LOCUS       DATLBZ010000001         3534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8945359, whole genome shotgun sequence.
ACCESSION   DATLBZ010000001 DATLBZ010000000
VERSION     DATLBZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8945359"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VMZ53_00005"
     CDS             <1..681
                     /locus_tag="VMZ53_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26847.1"
                     /translation="FADIVDQITAVFADATVIVGYNIAFDIDMLQAEYARIGRPMLDL
                     ANKQIVDAFKLWQACEPRSLQHAHQRFVGNGFASAHSASADVAATGRVLAGMLRHFSL
                     GPNWSQLATVCDPEGRLRDLLSRSTSWVGPSHHLRWDGDVIVVNFGKHANVPLHELAN
                     GPERSFLRWILDRPAGRDFPPHVNELCRAALELPPEELLAWARARFGIAAPTVEPPFA
                     ALARGTRG"
     gene            complement(703..1980)
                     /locus_tag="VMZ53_00010"
     CDS             complement(703..1980)
                     /locus_tag="VMZ53_00010"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HUS26848.1"
                     /translation="MRTALARALSVDDVNQLGRATGQSQRLRTVTPHRLFLSTIAALA
                     GGRVESLADLLREFNHQNDVTVAYKAFYNRLARPGFATFMRQMFARLVEQLCIQTLAP
                     EGHTAVARFKDIVIQDGSSFALKKQLRDVFPGRFTTIEPAAIELHATYSGFSDEVSAV
                     ELAPDKEAERQFLPAATTLKDRLLLADRGYPSVDYFEAVEKAGGSFIVRLSRSHDPWV
                     RAAWLDGKRVPLPEPIHLSRFIAQNSGRRMDLDVEYERGPGKQKRFVGFRVVVLPGNE
                     KTMTRLCTNLPRTPFSLDVVSRLYRFRWQVELLFKEWKSYANLHKFDTANEHIAAGLI
                     WASLCGAVLKRFLAHAAQLIGGKPISTRRVAMCSGHILDEIVAQLLACVSIATAFLDG
                     IAFLVANARRSNPKRDRKSGRLRTGLRVVGVRR"
     gene            2452..2775
                     /locus_tag="VMZ53_00015"
     CDS             2452..2775
                     /locus_tag="VMZ53_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUS26849.1"
                     /translation="MAFHAYDVTIELLHALRPILKRIEAREADLAKQLRRAAASTLQN
                     ICEANKRVNRDRANRFRVASGEAAEVKGSLEAAVAFEYIERREIAAAFERADRICAMT
                     YRLAK"
     gene            complement(3008..>3534)
                     /gene="lepB"
                     /locus_tag="VMZ53_00020"
     CDS             complement(3008..>3534)
                     /gene="lepB"
                     /locus_tag="VMZ53_00020"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HUS26850.1"
                     /translation="KRVIALPNDTVEIRCNVVYVNGKAIPNELVKAEDTYEDRSESDG
                     EMFSRHASRYRESHGGHTYDVYHDVERPQRKTGDPKDFPLDHVPNCANQMDYERQPAQ
                     NQSPGKVVETAPPSEGCGLYRHYQVPEGHVFGMGDNRNNSNDSRYWGSIPVENIKGRV
                     TGIWLPVGRFGGVD"
BASE COUNT          578 a         1172 c         1179 g          605 t
ORIGIN      
        1 ttcgccgaca tcgtcgatca gatcacggcc gtgttcgccg atgcgacggt gatcgttggc
       61 tacaacatcg cgttcgacat cgacatgctg caggccgagt acgcgcgtat cggtcgtccc
      121 atgctcgacc tcgcgaacaa gcagatcgtc gacgcgttca agctctggca ggcgtgcgag
      181 ccgcgcagcc tgcagcacgc gcatcagcgc ttcgtcggca atggcttcgc ctcggctcac
      241 tcggcgagcg cggacgtcgc cgccaccggt cgcgtgctcg ccggcatgtt gcgccacttc
      301 tcgctcggcc cgaactggtc gcagctcgcc accgtctgcg atcccgaggg ccgcctgcgc
      361 gacctactgt cgcgcagcac gagctgggtc ggcccatcgc atcatctacg ctgggacggc
      421 gatgtcatcg tcgtcaactt cggcaagcac gcgaatgtcc cgctgcacga gctggcgaac
      481 ggtcccgagc gcagcttctt gcgttggatc ctcgatcggc ccgccgggcg tgacttccct
      541 ccgcacgtca acgagctgtg ccgcgccgca ctcgagctgc cgcccgagga gctgctcgcc
      601 tgggcgcgcg cgcgcttcgg catcgcggca ccgacggtgg agccgccgtt tgccgcgttg
      661 gcgcggggca ccagaggtta ggggccgctc atacgtgatc acttaacggc ggactccgac
      721 gacgcgcagg ccggtccgga gtcgccccga ttttcggtcg cgcttcggat tcgagcggcg
      781 cgcgttggcg acgaggaacg cgattccgtc gagaaacgcc gtggcgatac tcacgcacgc
      841 gagcaactgc gcgacgatct cgtcgaggat gtgtcccgag cacatcgcga cgcggcgcgt
      901 ggaaatcggc ttgcccccga tgagttgggc ggcgtgggcg aggaatcgct tgaggacggc
      961 gccgcaaagg cttgcccaga tcagccccgc cgcgatgtgt tcgttggcgg tgtcgaactt
     1021 gtggaggttc gcgtaggact tccattcctt gaacagcagc tcgacctgcc agcgaaaacg
     1081 gtatagtcgc gagaccacgt cgagcgagaa cggcgtccgc ggcaggttcg tgcacagccg
     1141 cgtcatcgtc ttctcgttgc cgggtaggac aacgacgcgg aatccgacga accgcttctg
     1201 cttgcctgga ccgcgctcgt actcgacatc gagatccatg cgtcgtccgc tgttctgggc
     1261 gatgaaccgg gacaagtgaa tcggctcggg cagcggaacg cgcttcccgt cgagccacgc
     1321 ggcacggacc cacggatcgt gcgagcggga gagacgaacg atgaacgagc cgccggcttt
     1381 ctccacggcc tcgaagtagt caacgctcgg atagccgcgg tcggcgagaa gcaaccgatc
     1441 cttcaacgtc gtcgcggcgg gaaggaactg gcgctcggct tccttgtcgg gcgcgagctc
     1501 gacggcgctg acctcatcgg agaagccgct gtacgttgcg tgcaactcga tcgctgcggg
     1561 ttcgatggtc gtgaagcgtc cgggaaagac atcgcgcagc tgcttcttga gcgcgaacga
     1621 cgagccgtcc tggatcacga tgtccttgaa cctcgccacc gctgtgtgcc cctcgggtgc
     1681 gagcgtctgg atacagagct gctcgacgag ccgagcaaac atctgccgca tgaacgtggc
     1741 gaagcccggt cgggccagtc ggttgtagaa cgctttgtac gcgaccgtga cgtcgttctg
     1801 gtggttgaac tcgcgcagaa gatcggcaag cgattcgact cgtcctccgg cgagcgccgc
     1861 aatcgtcgag agaaagagcc ggtgcggcgt gaccgtacgc agtcgctggc tttggcccgt
     1921 agcgcgaccg agctggttga catcatcgac ggacagcgcg cgtgcgagcg cggtccgcat
     1981 cctgataagg tgcgaggtag acaacgcagt ctccgatctg cttggtgtgg taaccgcagc
     2041 ttggaggctg tcgttgtcgc ttgtcgatcc ccgggatctc gtcgcgcgat gccgacgtgc
     2101 agccacccgc tgcgagcgtc tcgacttcgc ccttgtagcg agcgattcac ctcggctgct
     2161 cgcgccgcag cggatctctc gtcgcgctgc ctcgcgtctg cacgtggtcg ccgccgccgt
     2221 gcgagcgtcg cgagctcggg cccgccgatg cgcacgctcg gccgtcgcga ggcggcgctt
     2281 cgatcgtgtc gtcggcgtgg tcgccacggt gccctgcctt cacgagccgc tccgtcgcgc
     2341 cgaggaccgc gccgcgcgcg cgaggtgtcg tggagaccgc gaacgctggt tcccagcgcg
     2401 cgtcacggtc ctggcgctcc aggagcggcc attcgtgaaa ggcaggtcac catggcattc
     2461 cacgcatacg acgtcaccat cgagcttctc cacgcactcc gtccgatctt gaagcgcatc
     2521 gaagctcgcg aagccgacct cgcgaagcag ctccgccggg cggcggcaag cacgctgcag
     2581 aacatctgcg aggccaacaa gcgcgtcaat cgcgatcgcg ccaaccgctt ccgcgtcgcg
     2641 agcggcgagg ccgccgaggt caagggctcg ctcgaagcgg cggtcgcatt tgagtacatc
     2701 gagcgccgcg agatcgccgc cgcgttcgag cgagcggatc ggatctgcgc gatgacctac
     2761 cgactggcga agtagctcgt cggcgaggcc gagcccgagg ccgaggccgg gccgaccccg
     2821 acgccgaggc cgaccccgac gccgaggccg accccgacgc cgagcccgag gccgagaccg
     2881 agccggagcc cgagccggag cccgagccgg agaccgatcc ggagaccgag ccggagcccg
     2941 agccggatgc gcccgcaccg gccgcaccgt cgagcgcagc cgttaagatc tcacctatga
     3001 ggggccgcta atcaacgccg ccaaagcggc cgaccggaag ccagatgcca gtgactcgac
     3061 ctttgatatt ctcgacggga atcgagcccc aataccgcga gtcattcgag ttgttgcggt
     3121 tgtcgcccat gccgaagacg tgaccctcgg gaacctggta gtgccggtag agcccacagc
     3181 cttcgctcgg cggtgccgtc tcgacgacct tgccgggact ctggttttgc gcgggttggc
     3241 gctcgtagtc catctggttc gcgcaattcg ggacgtggtc cagcgggaag tctttggggt
     3301 cgcctgtctt gcgctgcggt cgctcgacgt cgtgatagac gtcgtaggtg tgaccgccgt
     3361 gcgactcgcg atagcggctc gcgtgtcgcg agaacatctc gccatccgac tcggagcggt
     3421 cttcgtacgt atcctcggcc ttgacgagct cgttgggaat cgccttgcca ttcacgtaga
     3481 cgacgttgca acggatctcg accgtgtcgt tggggagggc gatcacgcgc ttga
//
LOCUS       DATLBZ010000002        13251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5220684, whole genome shotgun sequence.
ACCESSION   DATLBZ010000002 DATLBZ010000000
VERSION     DATLBZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13251
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5220684"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VMZ53_00025"
     CDS             complement(<1..382)
                     /locus_tag="VMZ53_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26851.1"
                     /translation="MRVTVFFMFVVGLAACGPNGRNNGSNCAGLCTALGYQACHEDGS
                     YDPPVSCGPDQMCDPNAGCVVCPPEGLYCGGATDNDVFRCNKQGTEGTLVESCAADEV
                     CSDGECKTPCEAAMDNPSNVGCDFW"
     gene            572..2041
                     /locus_tag="VMZ53_00030"
     CDS             572..2041
                     /locus_tag="VMZ53_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HUS26852.1"
                     /translation="MTSKLCLLLVIAAGCSASDESLSVRAVSPAIGSVTGGTRITLEG
                     TGLSSGTTVEIGGTPCESVNADGDSLSCVTGDAKFREGTVDVIVNRGGEQASLPAAFT
                     YECPWTTSSGRRSCGAAPARQTPEQTIAAWLSQQPMACATDGAATLSIQKQTGLPAVD
                     FRDSDFKIWVKVENVDHLASLDVWLGDASLANAFKFKLRSSQGQQWMTDGDWVSFTVP
                     WSPVGIVGAPNRAAITDVMIRAADDGASPVHVDVKGIAAVAQPMEHFPTGVVSFTFDD
                     NWADGMVAAPLLAAHRFAATEYAIIDTVGESDRASLADLRSLQESGWEIAVHSYTDEH
                     HAERFPNVTATELEDDLVEARAWLMQNGFTGYDHCAYPGGSFTGGKTDVLGLARRYFA
                     SCRTIYTTHHETFPPSDPQKLRTLYVTNGVALTTVKQAIDNARQTHEWIILVFHRFNP
                     MPTVTTEWSPADFAELVDYVAASGIKVQTVGDVLATESD"
     gene            complement(2025..3914)
                     /locus_tag="VMZ53_00035"
     CDS             complement(2025..3914)
                     /locus_tag="VMZ53_00035"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HUS26853.1"
                     /translation="MRALQIKVPFVAFALLLLAACGPNGRDSGGCPGICSALGYQACN
                     DGAYSEPVACGPEQTCDPNAGCVVCPPEGLYCGGPTNNDVFRCNKDGTDGTKVSSCDA
                     NSVCSNGECKTPCEAALDNASNVGCDFWAADLDNEAFNLNGISNDAAAQQFAIVAAND
                     NDYPVTVTVTKNAARVGQPLNEQAVASASVPPHGTTRIDLPQREVDGAMGQNGTYTKN
                     SGSGTFVSPHAFHVVSTGPVVVYQFNPIIQQFSNDASTLIPIQALGMDYVVIGYNTAN
                     PCGDPNFNIESIPDHTAITIAAVEDDTTVTVTPTHPIKASGGDSGVMIPATMAGQPVT
                     FKLSRYTVVNLESEQMVGNAFACAMSKQDGDFTGSQVHSDKPIVVFTSGERGIGFGGA
                     ENVQNPPGWDTSSGDDICCTDHLEEQLFPTTALGKEFAIARSPIRSTDPSWKEPDIFR
                     VVATVDNTLITTNLPAPYNSFTLNARQQKTFAATGGFALKASNAVEVASYLVSQHFVK
                     NGYTGDPSQLLHPAAEQHRKSYVFLVPATFQTNYIVLAKPKLASVVLDGVPLAQAETS
                     GCKYAPIGTVAGTEYEQVTCPASEGHHTVSADMPFGLSVYGYYNVGSYAFVGGSDVKI
                     INPIL"
     gene            complement(4228..4365)
                     /locus_tag="VMZ53_00040"
     CDS             complement(4228..4365)
                     /locus_tag="VMZ53_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26854.1"
                     /translation="MASLSVAAGEMRAAEGYLLELLAWCAFADRSIIEVAGDNEGPAQ
                     G"
     gene            complement(4551..7208)
                     /gene="treY"
                     /locus_tag="VMZ53_00045"
     CDS             complement(4551..7208)
                     /gene="treY"
                     /locus_tag="VMZ53_00045"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HUS26855.1"
                     /translation="MPKFEPRASYRLQLHAGFTFADALAAVPYFAELGISHLYLSPIL
                     TAAEGSTHGYDVVDPDRVSPVLGGDEGLKKLVDAAQAAGIGILLDIVPNHMSIAGHAN
                     QWWLDVLENGPSSYYAHHFDVDWASTEDRVLLPILTERYGRAIQNGAIKLVSAGNGFG
                     LEAGDVELPMAPHSIGHVVRRAADRIDRRALAALHGELAFIGDSLLALPRPHHGEVEA
                     RRRRHRDRKVLLGRLYALANELPASVALADELAAINSDPAELDTVLEMQAYRLAHWSV
                     STNQLSYRRFFDINTLVAIRNELPDVFEASHARILQLVEEGVLDGLRIDHVDGLRDPE
                     EYLQRLRERAPDAWIVVEKILTADETLPATWPIDGTTGYDFAEKLGGLFVDPESEAAM
                     TRLFEDYTGQPWNPHAGSRAARHEVMSDALHSELARLTELAVRACSESPTCRDYTRTE
                     IETALGEILAGYPVYRTYLTESRRSEIDRARIAAAAATAADERPDLDRDLLAFLEATL
                     AFEIASEPAKELARSAQQVTGAITAKGDEDTLLYRQVRLLARNDVGAELRSYAHSPSA
                     LQHFLASAKPRTLLATSTHDTKRSEDVRARIAVLSEIPDAWARVTARWRERTTRNWGD
                     VEPDRVIEYAIWQTLVGAWPVGTGSPADTFPRERLGDYVRKATREARLRTSWRKPDAA
                     YDAAIEKWLDGIYADPDVLTDIARFAQEIAPHGDRNSLAQLLIKLTAPGVPDFYQGTE
                     LRDDSLVDPDNRRAVDLAARQDRLRTIGDSSPGGLVGDLGAQKLWTIRRVLGLRRKVP
                     ARFLGAYKALDASGPHAHRVFAFMRGTELVAIVPRLTVRAEGWRDTALDLPTGTWTDV
                     LSDQQHSGGRRALADLWRSFPIALLTRTQ"
     gene            complement(7211..9343)
                     /gene="glgX"
                     /locus_tag="VMZ53_00050"
     CDS             complement(7211..9343)
                     /gene="glgX"
                     /locus_tag="VMZ53_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HUS26856.1"
                     /translation="MTTKRRPVWPGRTYPLGAHFDGYGTNFAVFTSVADKVELCLFDS
                     AGAEERIGLEQGIGQIWHAYLPEVGPGTRYGFRAHGPWEPQNGLRCNPHKLLVDPYAR
                     AISEGLQWGPALAGYKDGEPMGAMSEEDSAPYTLRSVVVQPFFDWGNDRRLEIPWNET
                     VIYEAHVKGLTYRHPEVPEKIRGTYAAVAHPAVIKHLKKLGVTAIELLPVHAFVHDGF
                     LLDRGQRNYWGYNTIGFFAPHPEYASVRDANGAVAEFKQMVRALHREGIEVILDVVYN
                     HTAEGNHMGPHLSMRGLDNGAYYRLVADNAGYYMDYTGTGNTLNARHPHTLQLVMDSL
                     RYWIEEMHVDGFRFDLAATLARGLHEVDRLGAFFDLIQQDPVVSRVKLIAEPWDVGEG
                     GYQVGNFPPGWSEWNGKYRDWIRDYWRGEMGTLPELGARFTGSSDLYQQGGRFPHASI
                     NFVTAHDGFTLNALVSYNGKHNDANGEESGESHNRSWNCGEEGETDNEEVKRLRARQQ
                     RNFLATLFLSQGIPMVLGGDEMGRTQQGNNNGYCQDSEISWFDWEHRDNDLVQFTRKL
                     SELRREHPVLRRRGWFQGRPIRPSQGKGPALPDIAWFSPEGVEMQEEHWDSAETRSLA
                     VFLNGAGILVPDDRGQPILDDTFLIIFHAHPEDRELTLPEKRWGATWQRVFDTDRGFA
                     KNGDGEKLDAGSKVQVLQRSLWVLRRIG"
     gene            complement(9362..10273)
                     /locus_tag="VMZ53_00055"
     CDS             complement(9362..10273)
                     /locus_tag="VMZ53_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HUS26857.1"
                     /translation="MLDWTLALAGGEGTRLQDYVQRRFGRRLPKQYCCLLGSRSMLQH
                     TLDRMNKLTPASRTLTVIGTHHGEIAAPQLVGRSEHVFRQPASRDTGLALYVAIAMIK
                     RWTPNAIITITPTDHYVAPSARYVDQVRQARGVAGRMRDKVVILGATPTEPDPELGYL
                     SLGAPLTELPQVKSVKGFIEKPPVDVCETLRKDGALWNTMVTCGTVDALWELGRATDP
                     RLIDILESFVPLVGTPDEDDAIEYIYRAYLPVSFSRDMLERAPERLAAMELEGVEWSD
                     WGRPERIETVLALRRSRALVPSRAVAP"
     gene            10553..11347
                     /locus_tag="VMZ53_00060"
     CDS             10553..11347
                     /locus_tag="VMZ53_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HUS26858.1"
                     /translation="MDERERTRELAAIIASGEAALFTGAGFSAEAFDRSGRSLPDSRE
                     MIAELWSQLFGDEPADDSSLADLYDVALIRAPERLRDYLRTRLQIGDSTLPPTFASWF
                     SAPWSRIYTLNVDDLEVAVARQFALPRPLVSVSALAKESPKSPENALEVVHLNGMASD
                     DPTQVTFSTMQYASRLCSEDHAYESLVEDLRSRSFVFVGTTLDEVVLWKHVQLERQRG
                     GGAPRKHSFLISPSLTRARQVLLESVKIHWIRGTAGEIAERVLARL"
     gene            complement(11598..13007)
                     /locus_tag="VMZ53_00065"
     CDS             complement(11598..13007)
                     /locus_tag="VMZ53_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS26859.1"
                     /translation="MAVDDLRSGTWVTYVGNRATSARLRRCRVEIVSGPDAGLIRDIE
                     ASVIRIGARRGNDVQLTDSKVSGLHCEIRLDDRGYRLRDLDSTNGTYVSSLRINDVYI
                     QPGTTISLGGTKFKFEPLGESVEIELADTDRFGSMIGRSVKMRELFARLEKLARTDTT
                     VLVTGETGAGKELVAEALHDHSPREKGPFVVLDCGSIPPNLIESELFGHERGAFTGAT
                     NSYAGAFERAHGGTVFLDEIGELPLGMQPKLLRVLERKEVRRVGGSKTIEVDVRICAA
                     TNRDLGVEVNRGRFREDLYYRLAVARVHVPPLRERKDDLPLLIEHILTTTPGGESASI
                     AQETIDLMMKHDWPGNVRELRNVIERAVLLAEAPDSEDSLRRAPAPAPKSEPSITVTP
                     SQTATSADASMTVPVDVGIPFKHAKQNVISEFERRYISRLLAQHDGNISAAARAAGID
                     RMSIHKMLHRLGLANPGRD"
BASE COUNT         2176 a         4322 c         4421 g         2332 t
ORIGIN      
        1 cccagaaatc acaaccgacg ttcgatggat tgtccatcgc ggcttcgcac ggcgtcttgc
       61 actcgccgtc cgagcagact tcgtcggccg cgcatgattc gacgagcgtg ccttcggtgc
      121 cttgcttgtt gcagcggaag acgtcgttgt cggtcgcgcc accgcagtag agaccttcgg
      181 gcgggcacac gacgcagccc gcattcggat cgcacatctg atcgggaccg cacgagaccg
      241 gtggatcgta actgccgtcc tcgtgacacg cttggtagcc gagcgccgtg cagagccccg
      301 cgcagttgct tccgttgttg cgcccgttcg ggccgcacgc cgctagaccg acgacgaaca
      361 tgaaaaagac ggtcaccctc atcgcgccga ccgtgcttca gctgcgtgtg cagctacaac
      421 gccaagtcgc gcatgcgcga gacgtgcagc acactgcgca tgggcgcacg accgagatat
      481 gcgcatggac gtcggcgcac gtcgatcctc gacgcatcgc gcagttaagc cgctccggcg
      541 tcggcactgc caatgcttcg tacgatggtc cgtgacctcc aagttatgcc tactcctcgt
      601 catcgcggcg ggctgttcgg cgagcgacga atcgctgtcc gttcgcgcag tctctccggc
      661 catcggttcc gttaccggcg gtacccgcat cacgctcgag gggaccggac tctcgtcggg
      721 cacgaccgtc gagatcggag gtacgccgtg cgaatcggtg aatgcggatg gtgactcgct
      781 gtcgtgcgtc accggcgacg ccaagttccg cgagggcacc gtcgacgtca tcgtgaatcg
      841 cggtggagag caggcgtcgc ttccggccgc gttcacgtac gagtgtccgt ggacgacgag
      901 ctcgggccgg cgcagctgtg gtgcggctcc cgcgcgccag acccccgagc agacgatcgc
      961 cgcatggctg agccagcagc cgatggcctg cgccaccgac ggggcggcga cgctctcgat
     1021 ccaaaagcag acaggcctgc cggccgtcga ctttcgcgac agcgacttca agatctgggt
     1081 caaggtcgaa aacgtcgacc acctcgcctc gctggacgta tggctcggcg atgcgagcct
     1141 cgcgaatgcg ttcaagttca agctgcggtc ctcgcagggc cagcagtgga tgaccgacgg
     1201 cgattgggtg tcgttcacgg tcccgtggtc gccggtcggc atcgtcgggg caccgaaccg
     1261 cgccgcgatc accgacgtca tgatccgggc cgccgacgac ggcgcgagcc ccgtgcacgt
     1321 cgatgtgaaa ggcattgccg cggtagcgca accgatggag cactttccga cgggcgtcgt
     1381 gtcgttcacg ttcgacgaca actgggcgga tggcatggtg gccgcgcccc tgctcgccgc
     1441 gcatcgcttc gccgccaccg agtacgccat catcgatacc gtcggtgaat cagaccgcgc
     1501 gtcgctcgcg gatctccgat ccttgcagga gtccggctgg gagatcgcgg tgcactcgta
     1561 taccgacgag caccacgccg agcgcttccc gaacgtgacc gctaccgagc tcgaggacga
     1621 cctcgtcgaa gcacgcgcgt ggctgatgca gaatggcttc accgggtacg accactgcgc
     1681 gtatcccggc ggcagcttca ccggcggcaa gacggacgtg ctcggcctcg cgcgccgcta
     1741 cttcgcgagc tgccgcacga tctacacgac gcaccacgag acgttcccgc cgtcggatcc
     1801 gcagaagctt cgcaccctct acgtgacgaa cggcgttgcg ctgacgaccg tgaagcaggc
     1861 gatcgacaac gcgcgccaga cgcacgagtg gatcatcctc gtgttccacc gcttcaaccc
     1921 aatgccgacg gtgacgaccg agtggtcgcc cgccgacttc gccgagctcg tcgattacgt
     1981 cgccgcgtcc ggcatcaagg tccagacggt cggcgacgtg ctcgctacag aatcggattg
     2041 atgatcttga cgtccgagcc gccgacgaac gcgtagctgc cgacgttgta gtagccatag
     2101 accgaaagcc cgaacggcat gtccgccgac accgtgtggt gaccttcgct cgcgggacac
     2161 gtgacctgct cgtattcggt gccggccacg gttccgatcg gggcgtactt acaaccgctc
     2221 gtctcggcct gcgcgagcgg cacgccgtcg aggacgacgg acgcgagctt gggcttcgcc
     2281 agcacgatgt agttggtctg gaacgtggcc ggaacgagga agacgtagct cttgcgatgc
     2341 tgctccgcgg ccgggtgcag gagctggctc ggatcacccg tgtagccgtt cttcacgaag
     2401 tgttgcgaca cgaggtagct cgcgacctcg acggcgttcg acgccttgag cgcaaacccg
     2461 cccgtcgccg cgaacgtctt ctgctggcgt gcgttgagcg tgaaggagtt gtacggcgcc
     2521 ggcaggttcg tcgtgatcag cgtgttgtcg acggtcgcga ccacgcggaa gatgtccggc
     2581 tccttccagc tcggatcggt cgagcgaatc gggctgcgcg cgatcgcgaa ctccttgccg
     2641 agggccgtcg tcgggaagag ctgctcctcg aggtggtcgg tgcaacagat gtcgtcgccg
     2701 ctgctcgtgt cccagcccgg cgggttctgc acgttctcgg cgccgccaaa gccaatgccg
     2761 cgctcgccgg acgtgaacac gacgatcggc ttgtccgaat ggacttggct gcccgtgaag
     2821 tcgccgtcct gcttgctcat cgcgcacgcg aacgcgttgc cgaccatctg ctccgactcg
     2881 aggttcacga ccgtatagcg cgacagcttg aacgtgacgg gttggccggc catcgtcgcc
     2941 ggaatcatga cgcccgaatc gccgcctgac gccttgatcg gatgcgtcgg cgtgacggtc
     3001 accgtcgtgt cgtcctcgac ggcagcgatc gtgatcgcgg tgtgatccgg aatcgattcg
     3061 atgttgaagt tcggatcgcc gcacgggttt gcggtgttgt agccaatcac gacgtagtcc
     3121 atgccgagcg cttggatcgg gatcagcgtc gatgcgtcgt tcgagaactg ctggatgatc
     3181 gggttgaact gatagacgac gacgggtccc gtcgacacca cgtggaacgc gtgcggcgac
     3241 acgaacgtgc ccgatccgct gttcttcgtg tacgtgccgt tctggcccat cgcgccgtcg
     3301 acttcgcgct gcggcagatc gatccgtgtc gtgccgtgcg gagggaccga tgcgctcgcg
     3361 acggcttgct cgttgagcgg ctgaccgacg cgcgctgcgt tcttcgtgac ggtgaccgtc
     3421 accggatagt cattgtcgtt cgccgcaacg atcgcgaact gctgcgcagc tgcgtcgttc
     3481 gagatgccgt tcaagttgaa cgcttcgtta tccagatcag ccgcccagaa gtcgcagccg
     3541 acgttgctcg cgttatcgag agccgcttca cacggcgtct tgcattcgcc gttgctgcag
     3601 acactgttcg catcgcagct cgagactttc gtcccgtcgg tgccgtcctt gttacagcgg
     3661 aagacgtcgt tgttcgtcgg tcctccacag tagagccctt cgggcggaca gacgacgcaa
     3721 cccgcattcg gatcgcaggt ctgctcgggc ccgcacgcaa cgggctcgga atacgcgccg
     3781 tcgttacatg cttggtagcc aagcgccgag cagatgcccg gacaaccgcc gctatcgcgt
     3841 ccattcggac cgcaggcagc gaggagaagt aacgcgaacg caacgaacgg aaccttgatc
     3901 tgtagcgccc tcatcgcggc ggaacgtcgt ccagacgccg agcccgcgtc aacgatgatc
     3961 tgcaaacctt cgcgcgtcgc gggcgatctg gtgcactacc tgtgcatgtc gatgcacaac
     4021 tctcgcggat tttctccgcg atagcggaga ggagaccgaa cgcactagac gagctcgatt
     4081 ctccgggtcg acacgcgtgg ccggcgcaca gccacggttc gcgacgaatt cgtcgtcaaa
     4141 ggtcgccttc ccctacccgg cctcgaacgg ctctccacgc aggagatccg cgatggctgc
     4201 ggcgcccacc ccgtctacga gccgtgatca gccctgcgca gggccttcgt tgtcgccggc
     4261 gacttcgatg atgctgcggt cggcgaacgc acaccacgcg aggagctcga ggaggtagcc
     4321 ctccgcggcc cgcatctcgc cggcagctac cgacagcgac gccatccaag aaagagtgcg
     4381 cgcgcgtgga cggagatcct gttcgagctc cgacgttggt gtggttgaca tcgacatgat
     4441 gtccacctgt tcttcaacga acgggccaaa acctcgctcg atcggccgat ctgcaagtgc
     4501 tcgattcggc accctcgagc acgtgcgccg gcgcgcaccc aacaagatcg ctactgagtg
     4561 cgagtaagaa gggcgatagg aaagcttcgc cagaggtcgg cgagtgcgcg ccttccgccc
     4621 gagtgttgct gatcagagag cacgtccgtc cacgttcccg tcggaagatc cagcgcagta
     4681 tcgcgccagc cctcggcgcg cacggtgagc ctgggcacga ttgcgacgag ctccgtgcct
     4741 ctcatgaacg caaacaccct gtgcgcgtgc gggcctgacg cgtcgagcgc cttgtatgca
     4801 ccgaggaatc tcgcgggcac cttccgacgg aggccaagga cgcggcggat cgtccacagc
     4861 ttctgcgcgc cgaggtcacc aacaagaccg cccggtgacg aatcgccgat cgtgcgcaga
     4921 cggtcctggc gagctgcgag atcgactgcg cggcgattgt ctggatcgac gaggctgtcg
     4981 tcgcgaagct cggtgccttg gtagaagtcg ggcacgcccg gcgcggtgag cttgatgagc
     5041 agctgtgcga gcgagttgcg atcaccgtgc ggtgcgatct cttgggcgaa gcgcgcgatg
     5101 tcggtgagca catcgggatc ggcgtagatc ccgtcgagcc acttctcgat cgcggcgtcg
     5161 tacgcggcgt caggtttgcg ccacgacgtg cgcagtcgcg cctcgcgcgt tgccttgcgc
     5221 acgtagtcgc cgaggcgctc gcgagggaac gtgtcagccg gcgaccccgt gccgaccggc
     5281 cacgcgccga cgagcgtctg ccagatcgca tactcgatca cgcgatcggg ctcgacgtcg
     5341 ccccagttgc gcgtcgtgcg ctcacgccag cgcgcagtga cccgtgccca cgcatcggga
     5401 atctcggaga gcacggcgat ccgcgcacga acatcttcgc tgcgcttcgt gtcatgcgtc
     5461 gacgtcgcga gcagcgtgcg aggttttgcg ctggcgagaa aatgctggag agccgacggc
     5521 gagtgcgcgt acgagcgcag ctctgcgccg acgtcgttgc gcgcgagcag gcggacttga
     5581 cgatagagga gcgtgtcctc gtcgcccttt gccgtgatcg cgccggtgac ctgctgcgcg
     5641 ctgcgcgcga gctccttggc aggctcgctg gcgatctcga atgcgagcgt cgcctcgaga
     5701 aacgcgagca agtcgcggtc gaggtcggga cgctcgtcgg cagcggtggc cgctgcggcg
     5761 gcgatgcgcg cgcgatcgat ttcgctgcgc cgcgactcgg tgaggtacgt gcggtagacg
     5821 ggatagcctg ccaggatctc gccgagcgcg gtctcgatct ccgtgcgcgt gtagtcgcgg
     5881 cacgtcggcg attcgctgca tgcccgaacg gccagctcgg tgagccgcgc cagctcggag
     5941 tgcagcgcgt cggacatcac ctcgtgacgc gcagcgcggc tgcccgcgtg cgggttccac
     6001 ggctgcccgg tgtagtcctc gaacaagcgc gtcatcgcag cttcgctctc gggatcgacg
     6061 aacaggccgc cgagcttttc cgcgaagtcg tagccggtcg tgccgtcgat cggccacgtt
     6121 gcgggcagcg tctcgtcggc ggtcaggatt ttctcgacga cgatccacgc atccggcgcg
     6181 cgctcgcgca agcgctgcag atactcctcg ggatcgcgca agccgtcgac gtgatcgatg
     6241 cgcagaccgt cgagcacgcc ctcttcgacg agctggagaa ttcgcgcgtg gctcgcttcg
     6301 aacacatccg gcagctcgtt gcggatcgcg accagcgtgt tgatgtcgaa gaagcggcga
     6361 tacgacagct gattcgtcga cacgctccag tgcgcgagcc ggtacgcctg catctcgagc
     6421 acggtgtcga gctccgcggg atccgagttg atcgcggcga gctcgtcggc gagcgcgacg
     6481 gatgcgggca gctcgttcgc gagcgcgtag aggcgaccga gcaagacctt gcgatcgcga
     6541 tggcgccggc gacgcgcctc gacctcgcca tgatgcggcc gcggcaacgc gagcagcgaa
     6601 tcgccgatga atgcgagctc gccgtgcagc gctgcgagag cgcgacgatc gatccgatcg
     6661 gcggcacgcc gcacgacatg accgatcgaa tgcggggcca tcgggagctc gacatcgccg
     6721 gcctcgagcc cgaacccatt gccggcggag acgagcttga tcgcgccgtt ctggatcgca
     6781 cggccgtatc tctcggtgag gatcggcagc agcacgcgat cttcggtgct cgcccagtcg
     6841 acgtcgaagt gatgcgcgta gtagctcgac ggtccgttct cgaggacatc gagccaccac
     6901 tggttcgcgt ggccggcaat cgacatgtga ttcggcacga tgtcgaggag gatgccgatg
     6961 cctgcggcct gcgccgcatc gacgagcttt ttcagtccct cgtctccgcc gagcacgggt
     7021 gagacccgat ctggatcgac gacgtcatag ccgtgcgtcg agccctcggc cgccgtgagg
     7081 atcggcgaca gatacagatg cgagatgccg agctccgcga agtacggcac tgcggcgagc
     7141 gcgtcggcga acgtgaagcc cgcatggagt tgcaagcgat acgacgcgcg cggctcgaat
     7201 ttcggcatgg tcaaccaatc cggcgcagca cccacagcga acgctggagg acctgcacct
     7261 tgctaccggc gtcgagcttt tcgccgtcgc cgtttttcgc aaacccgcgg tcggtgtcga
     7321 acacgcgctg ccacgtcgcg ccccagcgct tctcgggcag cgtcagctcg cgatcttcgg
     7381 ggtgcgcgtg gaagatgatc aggaacgtgt cgtcgaggat cggctggcct cggtcgtcgg
     7441 gcacgaggat gccggcgccg ttcaagaaca ccgcgagcga gcgcgtctcg gcgctgtccc
     7501 agtgctcttc ctgcatctcg acgccctcgg gcgagaacca cgcgatgtca ggcagcgccg
     7561 gacctttgcc ctgcgacggg cgaatcggcc ggccctggaa ccatccgcgt cggcgcagca
     7621 ctggatgctc gcggcggagc tcggacagct tgcgcgtgaa ctggacgaga tcgttatcgc
     7681 gatgctccca gtcgaaccac gaaatctcgg agtcctggca gtagccgttg ttgttgccct
     7741 gctgagtccg gcccatctcg tcgccgccga gcaccatcgg gatgccctgc gacaggaaca
     7801 gcgtcgcgag gaagttgcgc tgctggcgtg cgcgcagccg cttgacctct tcgttgtcgg
     7861 tctcgccttc ttcgccgcag ttccacgagc ggttgtggct ctcgccgctc tcttcgccgt
     7921 tcgcgtcgtt gtgcttgccg ttgtacgaga cgagggcgtt caacgtgaag ccgtcgtgcg
     7981 cggtcacgaa gttgatgctc gcgtgcggga agcggccgcc ttgctgatag aggtcgctgc
     8041 taccggtgaa gcgcgcgccg agctcgggga gcgtgcccat ctcgccgcgc cagtagtcgc
     8101 ggatccaatc gcggtacttg ccgttccact cggaccagcc gggcgggaag ttgccgactt
     8161 gatagccgcc ctcgccgacg tcccacggct cggcgatgag cttcacgcgc gagacgacgg
     8221 ggtcttgctg gatgagatcg aagaacgcac cgaggcgatc gacttcgtgc aggccgcgcg
     8281 cgagcgtcgc cgcgagatcg aagcggaagc catcgacgtg catctcctcg atccagtaac
     8341 ggagcgagtc catcacgagc tgcagggtgt gcgggtgacg cgcgttgagc gtgtttcccg
     8401 tgcccgtgta gtccatgtag tagccggcgt tgtcggcgac gagccggtag taggcgccgt
     8461 tgtcgaggcc gcgcatcgag agatgcggcc ccatgtggtt gccctcggcc gtgtggttgt
     8521 agacgacatc gaggatgacc tcgatgcctt cgcgatggag cgcgcgcacc atctgcttga
     8581 actcggcgac cgcaccgttt gcatcgcgaa cgctcgcgta ctcgggatgc ggcgcgaaga
     8641 aaccgatcgt gttgtagccc cagtaattgc gctggcctcg atcgaggagg aatccgtcgt
     8701 gcacgaatgc atgcacgggc agcagctcga tcgccgtgac gccgagcttc ttgaggtgct
     8761 tgatgacggc gggatgcgcg accgccgcat acgtgccgcg gatcttctcg gggacctccg
     8821 ggtggcgata cgtcaggcct ttgacgtgcg cctcgtagat caccgtctcg ttccacggaa
     8881 tctcgagccg gcggtcgttg ccccagtcga agaacggctg cacgacgaca gaccgtaacg
     8941 tgtacggggc ggagtcctct tcggacatcg cgcccatcgg ttcgccatct ttgtagccgg
     9001 ccagcgccgg tccccactgc aggccctcgc tgatcgcgcg cgcgtacgga tcgacgagca
     9061 gcttgtgcgg attgcagcgc aagccgttct gcggctccca cggcccgtgg gcgcgaaagc
     9121 cgtagcgcgt gcccggcccg acctcgggca gatacgcgtg ccagatctgg ccgatgccct
     9181 gctctagccc gatgcgctct tcagcgcccg cgctatcgaa caggcacagc tcgaccttgt
     9241 ccgcgacgct cgtgaacacc gcgaagttgg tgccgtaacc gtcgaagtga gcgccgagcg
     9301 ggtacgttcg ccccggccag acgggtcgac gcttagtcgt catgtcgacc cacgctccgc
     9361 gttacggagc gacggctcga ctcggcacga gcgcgcgcga gcggcgaagg gcaaggactg
     9421 tctcgatgcg ctcggggcga ccccagtcgc tccattcaac gccttcgagc tccatcgctg
     9481 cgaggcgctc gggggcgcgc tcgagcatgt cgcgagagaa gctgaccggc aggtatgcgc
     9541 ggtagatgta ctcgatcgcg tcgtcctcat cgggcgtgcc gacgagcggg acgaacgact
     9601 cgaggatgtc gatgaggcga ggatcggtcg cgcgaccgag ctcccacagc gcatcgacgg
     9661 ttccacacgt gaccatcgtg ttccagagcg cgccgtcttt gcgaagcgtc tcgcagacgt
     9721 cgacgggcgg cttctcgatg aagcccttca cgctcttcac ttgcggcagc tccgtcagcg
     9781 gcgcgccgag gctcaagtag ccgagctcgg gatcgggctc cgtcggtgtg gcgccgagga
     9841 taaccacctt gtcgcgcatg cgacccgcga caccgcgggc ttggcgaacc tgatcgacgt
     9901 agcgcgcgct cggcgcgaca tagtgatccg tcggcgtgat cgtgatgatc gcgttcggcg
     9961 tccagcgctt gatcatcgcg atcgcgacgt agagcgcgag gcccgtatcg cgtgacgcgg
    10021 gctggcggaa gacatgttcg gaccgaccaa caagctgcgg cgcggcaatc tcgccgtgat
    10081 gcgtcccgat gaccgtaagc gtgcgcgatg cgggggtcag cttgttcatg cgatccagcg
    10141 tgtgctggag catcgagcgg ctgccgagca ggcagcagta ctgcttgggc aggcgccggc
    10201 caaaccggcg ctgcacgtaa tcttgcaacc gagtcccctc accgcccgcc aacgccaacg
    10261 tccaatcgag cacggcgtca ctctcgccag ggcgagaagc ggacgtgcgc agcatacggt
    10321 cttcgagaag catcgttcgg cccttatgcg tttcccgtgc cgcacaccgt gctccgaatg
    10381 tccgctcggt agtcctcgga ctcagaggag ttgcgtaacg caacatggac cgggagcgtg
    10441 cgtcgaggcc ccagctgtgc gcggcagcgc gtgcgtgaaa tgttatgtgg tcaaaaggag
    10501 atccgacgtt gaagcgcgat ccccggcatg tctgctgcac caacgcacgg tagtggacga
    10561 gcgcgaacgc acacgcgagc tggcggcgat catcgcaagc ggcgaagctg cgctcttcac
    10621 aggcgccggc ttctcggccg aagcgttcga tcgctccggt cgctcgctgc ccgactcgcg
    10681 agagatgatc gccgagctgt ggtcgcagct ctttggcgac gagcccgccg acgacagctc
    10741 actcgccgac ctctacgacg tggcgctgat tcgcgcgccc gaacgcctgc gtgactactt
    10801 gcgcacgcgc ttacagatcg gcgattccac tctcccgccg acgttcgcga gctggttctc
    10861 cgcgccgtgg tcccggatct acacgctcaa cgtcgatgac ctcgaggtcg cggtcgcccg
    10921 ccaattcgcg ctgccgcgcc cattggtttc ggtctccgcc ttggccaagg aatcgccgaa
    10981 gtctcccgag aacgcgctcg aggtcgtgca cctcaacggc atggccagtg acgacccgac
    11041 gcaagtcacg ttctcgacca tgcagtacgc ctcgcgcctc tgctcggagg accacgcgta
    11101 cgagagcctg gtcgaggatc tgcgctcgcg ctcgttcgtg ttcgtcggaa cgacgctcga
    11161 cgaggtcgtc ctctggaagc acgtgcagct cgagcggcaa cgcggtggcg gtgcgccgcg
    11221 caagcactcg ttcttgatct cgccgtcgct gacccgcgcg cgccaggtgc tgctcgagag
    11281 tgtgaagatc cactggattc gcgggaccgc cggcgagatc gccgagcgcg ttctcgctcg
    11341 gctgtagctc cgcgggccgc atcggtcagt ctgagaaatg aacagggaga cacgaagcac
    11401 acggagcaaa gaggctctag ggaggctcac cggaacgcgt gcagcgagcc ctgcgggtcg
    11461 agccctgccc gtccaccttc gggctcgtcc accttcccgg tagccctgcc cgtccacctt
    11521 ccgggtctga ctccgtgtgc tccgtgtctc cctgtttcaa cctctgaggt tgctgagcgt
    11581 aggtacagag agagcggcta gtcgcggccg gggttcgcga ggccgaggcg atgcagcatc
    11641 ttgtggatcg acatgcggtc gatgcccgcg gcgcgcgcgg cggccgagat gttgccgtcg
    11701 tgctgcgcga gcaggcgcga gatatagcgg cgctcgaact cgctgatgac gttctgcttg
    11761 gcgtgcttga acgggatgcc gacgtcgacg ggcaccgtca ttgatgcgtc ggcgctggtc
    11821 gcggtctgcg acggcgtgac ggtgatcgaa ggctcggact tcggcgcggg ggccggcgcg
    11881 cggcgcagcg agtcttcgct atctggcgct tcggcgagca gcacggcgcg ctcgatcacg
    11941 ttgcgcagct cgcgcacgtt gcccggccag tcgtgcttca tcatgagatc gatcgtctcc
    12001 tgcgcgatcg acgcggactc gccgcccggc gtcgtcgtga ggatgtgctc gatgagcagc
    12061 ggcaggtcgt ccttgcgctc gcgcagcggc ggcacgtgca cacgcgcgac ggcgaggcgg
    12121 tagtagagat cctcgcggaa ccggccgcgg ttcacctcga cgccgagatc gcggttcgtc
    12181 gccgcgcaga tgcggacgtc gacctcgatc gtcttgctgc cgccgacccg gcggacttct
    12241 ttgcgctcga ggacgcgcag cagcttgggc tgcatcccga gcggcagctc gccgatctcg
    12301 tcgaggaaga cggtgccgcc gtgcgcgcgc tcgaaggcgc ctgcgtagct gttcgttgcg
    12361 ccggtgaatg cgccgcgctc gtggccgaac agctcgctct cgatcaggtt cggcgggatc
    12421 gagccgcagt cgaggacgac gaacgggccc ttctcgcgcg gtgagtgatc gtggagcgcc
    12481 tcggcgacga gctccttgcc tgcgccggtc tcgcccgtga cgaggacggt ggtgtccgta
    12541 cgcgcgagct tctcgaggcg cgcgaacagc tcgcgcatct tcacggagcg accgatcatc
    12601 gagccgaagc gatcggtatc ggcgagctcg atctcgacgg actcgccgag aggttcgaac
    12661 ttgaacttcg tgccaccgag cgagatcgtc gtgccgggct ggatatagac gtcgttgatg
    12721 cgaaggctcg agacgtacgt gccgttcgtc gagtcgaggt cgcgcagccg gtagccgcgg
    12781 tcgtcgaggc ggatctcgca gtggaggccc gagaccttgc tgtcggtcag ctggacgtcg
    12841 ttgccgcggc gcgcgccgat gcgaatgacc gatgcctcga tatcgcggat gagccccgcg
    12901 tccgggccgc tgacgatctc gacacggcag cgccggagtc gggcgctcgt cgcgcgattt
    12961 ccgacgtagg tgacccaggt tcccgatcgc aggtcatcga cggccatgcg ggcggcacgt
    13021 taccacaagg tccggagttc tccgtccagt atgtagactc tcgtagagcg gctcgaaagt
    13081 gcccttttaa ggcattacac cgacggagat cgacaagcag tcctcgcgag cgaccgccat
    13141 gtgccaccac cgcgagggga tgtagaggaa gtcgcctggc gccagcaccg ccgtctgtag
    13201 cggcgaggtc tcgcggtgga aattcgagaa gtcggccggc ggaaacggcg t
//
LOCUS       DATLBZ010000003        13470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7455031, whole genome shotgun sequence.
ACCESSION   DATLBZ010000003 DATLBZ010000000
VERSION     DATLBZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13470
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7455031"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VMZ53_00070"
     CDS             complement(<1..1333)
                     /locus_tag="VMZ53_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26860.1"
                     /translation="MSETPTPIGESSTIFLIYSYEAEGAGPDFDLDAFERQLCANDRW
                     NLKRLDDTALLPHLDAIREGQLLRRYYVGSQLGNLFSERDGPWTLHGEGSAKVQVRIS
                     EAPSVYAFETGYVFIAIGVQPVDADLATYQNTLNALVRRGVHGLREGSLPKQANAWTE
                     KHAASARTLRTWLAALVPALKLLIPEGGRENPVSVGALFCETPLDALDRHRLRLAQAH
                     SQDIPPSEHDARTQDHPAIWCPSANEMCLFSSLGVQWVIKPSVPFLRNNLLGMIGSSY
                     LYKWMLVAHQRIYLLRLTSSAVEVSRRGGVQGADRLRVALLRYTAVINCGHISDEERH
                     DQFYGAIRRALDIEGLYEEAKEEITEIHEHAGARQAERINHVLAFLTIVLTPIGMVVG
                     IFQGDTLPWPPGQFKLGYLFSWSGWSKLLDHGPFRLLLLCAVIGVALFLFFR"
     gene            complement(1330..2979)
                     /locus_tag="VMZ53_00075"
     CDS             complement(1330..2979)
                     /locus_tag="VMZ53_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HUS26861.1"
                     /translation="MGNAALPANWRVRWSLEPSSDGLGIFMRALVVDAKSVSMAALEH
                     GLAAWGRSEDKLGATTIDIPQIPVLDDIWCLYMLAARERGEAKHSGWDAMSHYVEDVR
                     RGYWPDRVKPEQAVQAVFSAIVHEHLAGATPTRDEFLADALELCRHVDAKLACGADLL
                     DSDVTDAPKLQRYRAALASDHKLYDEDVERSMRWFARIPAERADGRERKVPLLVLRSP
                     LARQFKMWARSDLRAPGGHGYPLLLVDAGKGEVVLSADPTSKLKVGWLAAGLDTLEGG
                     DAHWYDGARHGFTLVAAPKGGTRLSLDEVVAYLRKELSFGRVWNHRRIRMASIAAAAV
                     LVMGGSVAIVVHTLQKDPGKDIADANHQALYLAILPGTEPCTAFVIGTHELATAGHCL
                     ERGETLLIHNDDPKDQRKVIRSYRHKEYPLVDVGVFVVADPPLPKGVDLADEPGGQGA
                     SIYIFAFVQERLNRKHPRARMDHTTIAFEPTKTQFEHSAATSGGSSGGPIFNADGKVI
                     AIHTNVAADKFGKATPFAGGIPITRLHELRSHKDLQVEVMR"
     gene            3079..3645
                     /locus_tag="VMZ53_00080"
     CDS             3079..3645
                     /locus_tag="VMZ53_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26862.1"
                     /translation="MLLLACSGKRDKPRADIDDAGGGSTILQHGDPELSRKPEEAVKA
                     VDALVWSIGISQPLMLLEHTPTANQPPFCQDGKALLNKLFDDIKADTASQCTPSMSAQ
                     TDELAIQCRLNIAGECTYFYRATLWTEWDRAIDHKYRLLGTAWLQESFCGDARKRADA
                     DPNLLFRVQIGKSFRALVDQCRSSGTSP"
     gene            3642..4697
                     /locus_tag="VMZ53_00085"
     CDS             3642..4697
                     /locus_tag="VMZ53_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26863.1"
                     /translation="MKLITSSLLLVGAVSVGCGGGSKASTLVEVIAKRTDDFPQTHRV
                     VVPLSGRNSLGSSVDELPERRITPRYNDCFFVVEQQAKGVDKITQKFTGSAAVKSEFA
                     AVAASAGGELKSEDEVVVEFNGITVYGGYGVPNLIAPCGKLENGSKIQVITEQIRAAS
                     AQVQFSHSFVARIGAGGGASKAQAQVAAGWSESTKGQVLGKDIVLTARVDEVTLTVEN
                     ANGQPIPDDVETGTAFALPKSINELGVLTVQRNDVPGKSVTIRFQPKLGGNFLVDPKR
                     TSGVPANLCTINEDHALTANQGCVFWLTPGNAALSVSWAIKDGKKLLSATYYRTTMEP
                     ARTNVAADELREKLASH"
     gene            4774..5748
                     /locus_tag="VMZ53_00090"
     CDS             4774..5748
                     /locus_tag="VMZ53_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26864.1"
                     /translation="MSGLRACVLAVLVAACGEVNIIADATTQSDAPDLCTTMTCDDND
                     ACTMDTCANNACVFTPLPAPTGSQAFSYTGTITTFAVPECVKTITIEASGARGGNGSN
                     LGGLGAKMKGTFVVVPGTELSIIVGGTGGDALTTGGGGGGSFVWATQNATMPLIVAGG
                     GGGGSSHSAGAPGLKAPDGGNGVNLTTGGGIGGNGGTVPTALAWAAGGAGWLSNGNGA
                     GGNAGTCGIAVGGSRAVTGGAGGVFGGTAGNVGAGGFGGGGGGQGRCNAVGGGGGGGY
                     SGGGPGGEANGMSIMYHGGGGGGSYNGGTSQENADGVNNGAGSVAITW"
     gene            5760..7607
                     /locus_tag="VMZ53_00095"
     CDS             5760..7607
                     /locus_tag="VMZ53_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HUS26865.1"
                     /translation="MASEDPEAVWLRDVYQPNATNLTIRAVIVGMLIGGVMCISNLYV
                     FFKTGWSSGVTLTACILAFAVFEAGRAIGIVRKPLTVLENNALTTVASGAGYMTGGGN
                     MAAYGALVMLIATTPSLALTTPDPAPMIAWFAVIATLGVFVAIPIKRQLINRDGLAFP
                     TGTATATTIKAIHEAGTKEKGSGPSQAAWLGIGALAGAAIAWVRDAMAVVPTVLPAFG
                     ATAAKWTLSIKTELILFGFGALMSFRTGWSMLLGALATYAVIGPILLDRGLITLDKGV
                     YKGVVAWTLWPGAAILVGAGLTSLALDYKAVARSFTGLGAAFKRQRGEATGIGAVEAP
                     DWWFPAAVLLLSPIIVFLMHHLFDIPVWAGILAIPLAVVMGFIAARVTGETDVTPTKA
                     LGPVTQMIYGVITPGNMTGNIMSANVTGGIGLHAADLLTTLKTGWLLGAKPRHQLYAQ
                     LFGVLAGAAVVVPAFLLIIPQPTPDQAAHGITTVLGTQEWPAPSSVVWAGVSEAFSNG
                     IGKMHHTVKVAIGIGFAIGIGLTIFEKLAPKHVKPFIPSANGIGIAMVLPGFNSIAMF
                     TGAAIAEILRRGQRSSAVMPVASGIIAGESLMGVLIAVLTTIVGLDLKL"
     gene            complement(7597..7983)
                     /locus_tag="VMZ53_00100"
     CDS             complement(7597..7983)
                     /locus_tag="VMZ53_00100"
                     /inference="COORDINATES: protein motif:HMM:NF025607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HUS26866.1"
                     /translation="MPRGNLRTLQVIVGLAVVLVGGVLVFSPTAVADALGKPHDTPTR
                     MINLRASWGGTLAGLGAYIAWLPGWSPRWRAFAGLVMWAMAGIGAARLVGFALDGSPD
                     TRQYIWIAAEVALVIGCAVALLRPKA"
     gene            8059..9102
                     /locus_tag="VMZ53_00105"
     CDS             8059..9102
                     /locus_tag="VMZ53_00105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS26867.1"
                     /translation="MQPTGQLIDGKYELVAVAGKGGMATVYKAMQKGAAGFQRPVAIK
                     KIKPEYRALKNYVDMFIEEARVGSELAHPNIVQVHDFVAQDGSYYLVMEWVEGIDLGL
                     FLKLHRDAGIHVEWPLVVAIGIGTLRGLGAAHDRRGPNGEPSPVIHRDVTPHNVLLGV
                     NGVVKLTDFGLARARDRVASMTAPGTVKGKLSYLAPEVSFGKANTPQSDLFCVGSVLW
                     ETLSGERLFDGKTDVEIFKKIRACKVPPIQERRPDVPPSLAAVLDVALDIDPNNRFAS
                     ATAFAHALAQVMKQAVGIDPQSALGAAVRELRATGKAGTSSAATSDYDLSKADMDEAP
                     LELTNPKKTKPPI"
     gene            complement(9103..9963)
                     /locus_tag="VMZ53_00110"
     CDS             complement(9103..9963)
                     /locus_tag="VMZ53_00110"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HUS26868.1"
                     /translation="MALYVIGDIQGCMASLERLLSLFDFSPQSDRLWLVGDLVNRGPR
                     SLDVLRWAYEHDDVITCVLGNHDIHLLARYAGAAPEKKRDTLDDILNARDAGKLIDWL
                     RARPLLHVDAGSRRAPTSPGAPSVARVAMVHAGLHPEWTIDDARARAGEIETQLRAPT
                     WRAFLAQLKGSAPRWKPSLGGGDRWRAILAYLTRARMLKADGRADDDFNGAPKDAPSD
                     LTPWFKFPNAAWQTHTVVFGHWAALGLDLGPHHIALDTGCVWGKSLTAFRLDDRSAFQ
                     AKAVEPPRNE"
     gene            complement(9963..11672)
                     /locus_tag="VMZ53_00115"
     CDS             complement(9963..11672)
                     /locus_tag="VMZ53_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HUS26869.1"
                     /translation="MARALADLSRGFRQSYLDDAQLNGQLKAWADAYPSLVRLTSLAK
                     TPEGRDVWLVTIGPEPDRIRPAVWADGNVHAAELAGSSVALSIAEDALRAHLEPDSLD
                     LPQPIIDRMREVLFYVVPRVSPDGAECVLKTGRTVRSVPRDSRVHQGQPRWILKDMNG
                     DGLALAMRVKDPGGELVETPQFPGLLVERTIEDEGPFYKVYPEGVIEHFDGKTIPSPF
                     FLGDNPIDLNRNFPWFWAPHHEQIGAGPFPASEPEARGIIEFTSAHPEIFAWVDYHTF
                     GGVLIRPLGHGPDSKMNQEDLAIFRQIEQWMTEHTGYPTVSGHDEFLYEPDKPLRGDL
                     SDYGYNQRGALAYVVELWDLFKRLGMERPPKFVQYYERVSRADIIKFAWWDRDENEGR
                     CFPGWKPFEHPQLGPVEIGGIDPRIGMWNPPPHELPALCASQAHVFVRLAALAPRLRI
                     TRTDRSALPGGLVRVEVQVTNEGYLGTYGAPSAKPLDFNEPLYAVAKGPLVEGSKAHQ
                     TLGHLDGWGHGLHTGANMPAYPGTRGTTNAAWATYLVKGGGTLDVQIGSARTGFITAR
                     IEV"
     gene            11742..11963
                     /locus_tag="VMZ53_00120"
     CDS             11742..11963
                     /locus_tag="VMZ53_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26870.1"
                     /translation="MPMLSFDSLPQARVVRKGGQHFAPPPAPLTYEQSAMREKMHDAL
                     ALMIGLWPLTAIVLLCVGLIIMAGNNGAP"
     gene            complement(12025..12909)
                     /locus_tag="VMZ53_00125"
     CDS             complement(12025..12909)
                     /locus_tag="VMZ53_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26871.1"
                     /translation="MFATRVYHYRDPAAVILGLKELRKQGLTPRGLLFIALDPRGETY
                     IAVPEDLDAVASIKVGDKLSLVPPWEGRYFNFDAVHRLPGDTVLWNGDRRLAETGSAS
                     EVATAISEWLKGSSAKNVFLGCAPHVPGSWWTINERSRVVALHELGYLDCVVTTTGIL
                     ARKIDDARLFHLDWQSLREHAAPTEGWQEVFTSQMGNVLLVERRVLQYRLVLTCERGL
                     VEIDVSHLPDLVIETARVPMRSGFGVVGRIDGGAFAVTAGTIEPWGLTNMSPAMLVGS
                     PTESLLDLPKALRAMPLE"
     gene            complement(12920..>13470)
                     /locus_tag="VMZ53_00130"
     CDS             complement(12920..>13470)
                     /locus_tag="VMZ53_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HUS26872.1"
                     /translation="RDMLSKRGRWLVLLLVGEMLTTTAMGFFEDQLKAAIVLTLFIPL
                     IVSAGGNSGSQASTLVIRAMALGEIRSADWWLVMRRELAMGLALGAVLGMVALVRVLI
                     WGTFGVYGDHYFLVAAVVGGAVLGCVLSGTLAGSMLPFLLRKVGADPASASAPFVATL
                     VDVTGIVIYFTIAHILLKGVLL"
BASE COUNT         2370 a         4353 c         4496 g         2251 t
ORIGIN      
        1 tgcggaagaa caagaacagc gcgacgccga tcacggcgca cagcagcaat agccgaaacg
       61 gcccgtggtc gagcagcttg ctccagccgc tccacgagaa caggtaaccc agcttgaact
      121 ggcccggcgg ccacggcagc gtgtcgccct gaaagatgcc gacgaccatg ccgatcggcg
      181 tgagcacgat cgtcagaaac gcgagcacgt gattgatgcg ctccgcttgg cgcgcgccgg
      241 cgtgctcgtg gatctcggta atttcttcct tggcctcttc gtacaggccc tcgatgtcga
      301 gcgcgcggcg gatcgcaccg tagaactgat cgtggcgctc ctcgtcgctg atgtggccgc
      361 agttgatgac cgcggtgtag cgaagcagcg cgactcgcaa ccggtcggca ccctggacgc
      421 cgccgcgacg actgacctcg acggcggagc tcgtcagccg caacaagtag atccgctgat
      481 gtgccacgag catccacttg tagagatacg agctgccgat catgccgagc aggttgttgc
      541 gcagaaacgg cacgctcggc ttgatgaccc actgcacgcc gagcgacgag aacaaacaca
      601 tctcgttcgc cgacggacac cagatcgcgg ggtggtcctg cgttcgtgcg tcgtgctccg
      661 acggaggaat gtcctggctg tgcgcctgcg cgagccgcag acggtgacga tcgagcgcat
      721 cgagcggcgt ctcgcaaaac aaagcgccga ccgacaccgg gttttcgcgg ccaccctcgg
      781 ggatgagcag tttcaaggca ggcacgagcg cggcgagcca cgtgcgcaac gtcctcgcac
      841 ttgccgcgtg cttctccgtc caggcgttcg cctgtttcgg cagcgagcct tcgcgaaggc
      901 cgtgcacacc acgacgaacg agcgcgttga gcgtgttttg atacgtcgct aggtcagcgt
      961 cgacgggttg cacgccaatc gcgatgaaca cgtacccggt ctcgaacgcg tagaccgacg
     1021 gcgcctcgga gatacgcact tgcactttag cgctgccttc gccgtgcagc gtccacggtc
     1081 catcgcgctc cgagaacaaa ttgccgagct gcgaccccac gtagtagcga cgaagcagct
     1141 gcccctcgcg aatggcgtcg aggtgcggca gcagcgccgt gtcgtcgaga cgcttgaggt
     1201 tccagcgatc attcgcgcac agctgtcgct cgaacgcatc gaggtcgaaa tcgggtcctg
     1261 cgccctccgc ctcgtacgag tagatcaaga atatcgtcga cgactcgccg atcggcgtgg
     1321 gcgtctcgct caccgcatca cctctacttg caggtccttg tgcgaacgca gctcgtgcaa
     1381 acgagtgatc ggaattccgc ccgcgaacgg cgtcgccttt ccaaacttgt cggccgcgac
     1441 gttcgtatgg atcgcgatga ccttgccgtc cgcgttgaag atcggtccac ccgagcttcc
     1501 gccgctcgtc gcagcactgt gctcgaactg agtcttggtc ggctcgaagg caatggtggt
     1561 gtggtccatg cgcgccctcg gatgcttcct gttcagacgc tcctgaacaa acgcgaagat
     1621 gtagatgctc gctccttgcc cgcccggttc gtcggcgagg tcgacccctt tgggaagcgg
     1681 tggatccgca acgacgaaca cgccgacgtc gacgagggga tactccttat gtcgatagct
     1741 gcggatcacc tttcgttgat ctttcgggtc gtcgttgtgg atcagcagcg tttctccgcg
     1801 ctcgaggcag tggcccgcgg tcgcgagctc gtgtgttccg atgacgaagg ccgtacacgg
     1861 ctcggtgccc ggcaagatcg cgagatacag cgcctgatgg ttcgcatctg cgatgtcctt
     1921 tcccggatct ttctggaggg tgtggacgac gatcgcgacc gaaccaccca tcacgaggac
     1981 ggcagcagcg gcgatgctcg ccatgcgaat ccggcggtga ttccagaccc tgccgaacga
     2041 gagctccttg cgcaaatacg caacgacctc gtcgagggac agccgggtcc cgcccttcgg
     2101 tgcagcaacg agcgtgaagc cgtggcgagc gccgtcgtac caatgcgcat cgcctccctc
     2161 gagcgtgtcg agtcccgcgg cgagccagcc gactttgagc ttcgacgtcg ggtccgcgct
     2221 gaggacgacc tcgcccttgc cggcgtcgac gagcaacagt ggatacccgt gcccaccggg
     2281 cgcgcgcaga tcggagcgtg cccacatctt gaattggcgc gcgagaggcg agcgaagcac
     2341 gagcaacggc accttccgct cgcgcccatc ggcgcgttca gcgggaatcc gcgcgaacca
     2401 ccgcatgctc cgttcgacgt cttcgtcgta gagcttgtga tccgacgcga gcgccgctcg
     2461 ataccgctgg agcttcggcg catcggtcac gtcggaatcg agcaggtcgg cgccgcacgc
     2521 gagctttgca tcgacgtgac gacacagctc gagcgcatcg gcgaggaact cgtcgcgcgt
     2581 tggagtggct ccagcgaggt gctcgtgcac gatcgcgctg aagactgcct gcaccgcctg
     2641 ttcgggcttc acgcgatcgg gccagtagcc tcgacgcacg tcctcgacgt agtgagacat
     2701 cgcgtcccaa ccgctgtgct tggcttcgcc cctctcgcgc gccgccagca tgtacaagca
     2761 ccagatatcg tcgagaactg gaatctgcgg gatgtcgatc gtcgtcgcgc cgagcttgtc
     2821 ttcgctgcgt ccccacgcag caagtccatg ctcgagcgca gccatcgaca ccgacttcgc
     2881 gtcgacgacg agcgctcgca tgaagatgcc gagaccgtcg gagctgggtt cgagcgacca
     2941 acgcactcgc cagtttgctg gcagtgccgc attccccatc aacgaaccat aacactgaag
     3001 ctcgcgctct tgcgctcgca cgcggcgcgc gggcatcatg gccggcactc gatgcgcagg
     3061 ttgtcgttag tcgccgccat gctcttgctc gcgtgcagtg gaaagcgaga caaaccgcgg
     3121 gccgacatcg acgatgcggg cggcggcagc accatcctgc agcacggcga tcccgagctc
     3181 tcgcgcaagc ctgaagaggc cgtgaaagcg gtcgacgcgc ttgtgtggag catcggtatt
     3241 tcccagccac tcatgctgct cgagcacacg ccgaccgcga accaaccgcc gttctgtcag
     3301 gacggcaagg cgctgctgaa caagctcttc gacgacatca aggccgacac agcgagccag
     3361 tgcacgccga gcatgtccgc gcagaccgac gagctcgcga tccaatgccg gctcaatatc
     3421 gccggcgaat gcacgtactt ctatcgcgcg acgctgtgga ccgagtggga tcgcgcgatc
     3481 gaccacaagt accggttgct cggaaccgcg tggttgcaag agagcttctg cggcgatgcg
     3541 cgcaagcgcg ccgacgctga ccccaatctt ctcttccgcg ttcagatcgg gaagagcttt
     3601 cgcgcactgg tcgaccagtg ccggtcatca ggaacatcac catgaaactc atcacgtcta
     3661 gcttgttgct cgtaggtgcg gtgtccgtcg gttgcggcgg tggctcgaaa gccagcacgc
     3721 tggtcgaagt catcgcgaaa cgaaccgacg actttccgca aacccatcgt gtcgtcgtgc
     3781 cgttgtcagg gcgcaactcg ctcggttcgt ccgtcgatga gctgccagaa cgtcgcatca
     3841 cgccgcgtta caacgactgc ttcttcgtcg tcgagcaaca agccaagggc gtcgacaaga
     3901 tcacgcagaa attcacgggc agcgcggcgg tgaaatcgga atttgcggcc gtcgctgcct
     3961 ccgcaggtgg cgagctcaag tcagaagacg aagtcgtcgt ggaattcaac ggcatcacgg
     4021 tctacggcgg ctacggagtt ccgaacctga tcgcgccgtg cggcaagctc gagaacggct
     4081 cgaagatcca ggtcatcacc gagcagatcc gcgccgcgtc cgcgcaagtg cagttctccc
     4141 actcgttcgt cgctcggatc ggcgccggcg gcggtgccag caaagcacaa gcgcaggtcg
     4201 cggcgggctg gagcgaatcg acgaaggggc aggtgctcgg taaggacatc gtgctcactg
     4261 cgcgcgtcga cgaagtgacg cttaccgttg aaaacgcgaa cggccaaccg atccccgacg
     4321 acgtcgagac cggaaccgcg tttgcgctgc cgaaaagtat caacgagctc ggcgtcctca
     4381 ccgtgcagcg caatgatgtg cccggcaaga gtgtgacgat ccgctttcaa ccgaagctcg
     4441 gcggcaactt cctcgtcgat ccgaagcgca cctcgggtgt tccggcgaac ctgtgcacga
     4501 tcaacgaaga ccacgcgctc actgcgaacc aaggctgcgt gttctggctg acgcccggta
     4561 acgctgcgct ctcggtgagc tgggcgatca aagacggcaa gaagctcctg tcggcgacgt
     4621 actaccgcac gacaatggag ccggcccgca cgaacgttgc ggccgacgag cttcgcgaga
     4681 agctcgcgtc gcactagcgt cgggcgatcg cctacatgcc gtctggtgcg caggacgtgg
     4741 ccttccgcca gcgcgtcgcg ataagatggc gccatgtctg gtcttcgtgc gtgtgtccta
     4801 gccgtgctcg tcgctgcctg cggcgaggtg aacatcatcg cggatgcaac gacgcagagc
     4861 gatgcgccgg atctctgcac gacgatgacg tgcgacgaca acgacgcatg cacgatggac
     4921 acgtgcgcga acaacgcgtg cgtgttcacg ccgctcccgg cgccgacggg ctcgcaagcg
     4981 ttcagctaca cggggaccat cacgacgttc gcggttcccg aatgcgtcaa gacgatcacg
     5041 atcgaggcga gcggcgcgcg tggcgggaat gggagcaacc tcggcggtct gggcgcgaag
     5101 atgaagggaa cgtttgtcgt ggtgcccggc accgagctgt cgatcatcgt cggtggcacc
     5161 ggtggtgatg cactcacgac gggcggaggt ggtggcggtt cgttcgtttg ggccacccag
     5221 aacgcgacga tgccgctcat cgtagcgggc ggtggtggcg gaggcagctc gcactcggca
     5281 ggtgcgccgg gtctcaaggc gccggatggc ggcaacggtg tgaatctcac gacgggcggc
     5341 ggcatcggcg gtaacggtgg aaccgttccg acagcgcttg catgggccgc gggcggcgcg
     5401 ggctggctgt cgaatggcaa cggtgcgggt ggaaacgcgg gcacgtgcgg aatcgcggtg
     5461 ggtggctcgc gtgcggtgac cggcggcgcc ggcggcgtgt tcggcggcac ggctggcaat
     5521 gtcggcgccg gcggattcgg cggtggtggc ggcggtcagg gccgttgcaa cgccgtcggt
     5581 ggcggcggcg gcggcggcta cagcggtggc ggccccggcg gtgaggcgaa tggaatgtcc
     5641 atcatgtatc acggtggtgg tggcggcggt tcgtacaacg gcggtacctc gcaagagaac
     5701 gccgatggcg tgaacaacgg cgccggttcc gtcgcgatca cctggtagct tcgcgttcga
     5761 tggcatctga ggatcccgaa gccgtatggc ttcgcgacgt atatcagccg aacgcgacga
     5821 acctgacgat ccgcgccgtg atcgtcggca tgttgatcgg cggcgtcatg tgcatctcga
     5881 acctctacgt cttcttcaag acgggatgga gctcgggcgt gacgctgacg gcgtgcatcc
     5941 tcgcgttcgc cgtgttcgaa gcgggccgcg cgatcggcat cgtccgcaag ccgctcacgg
     6001 tgctcgagaa caacgcgctg acgaccgtcg catctggcgc aggctacatg acgggcggcg
     6061 gcaacatggc agcgtatggc gcgctcgtca tgctgatcgc gacgactccg tcgctcgcgc
     6121 tcacgacgcc cgatcccgcg ccgatgatcg cgtggtttgc agtgatcgcg acgctcggcg
     6181 tgtttgtcgc gatcccgatc aagcgccagc tcatcaatcg ggacggcctc gcattcccga
     6241 cgggaacggc gaccgcgact acgatcaaag cgatccacga agccggtacg aaagaaaaag
     6301 gcagcggccc atcgcaagcc gcgtggctcg gcatcggcgc gctcgcgggt gcagcaatcg
     6361 cgtgggtgcg cgatgcgatg gctgtcgtgc cgaccgtgct tcccgcgttt ggcgcgactg
     6421 ccgcgaagtg gacgctctcg atcaagaccg agctgatcct gttcggcttt ggcgcgctga
     6481 tgagcttccg caccggctgg tcgatgctcc tcggcgcgct tgcaacgtac gcggtgatcg
     6541 gcccgatcct cctcgaccgg ggcctcatca cgctcgacaa aggtgtctac aaaggtgtcg
     6601 tcgcgtggac gctgtggccc ggggcggcga tcctcgtcgg cgcggggctg acgtcgctcg
     6661 cgctcgacta caaggcggtc gcgcgatcgt ttaccggcct cggcgcggca ttcaagcgcc
     6721 agcgcggcga ggcgacgggc atcggtgccg tcgaagcgcc ggactggtgg ttccccgcag
     6781 cggtgctcct gctgtcgccg atcatcgtgt tcttgatgca ccacctgttc gacattccgg
     6841 tgtgggcggg catcctcgcg atcccgctcg ccgtcgtgat gggcttcatc gcggcccgcg
     6901 tgaccggcga gaccgatgtg acgccgacga aagcactcgg cccggtcacg cagatgatct
     6961 acggcgtgat cacgcccggc aacatgacgg gcaacatcat gtcggccaac gtcacgggcg
     7021 gcatcggcct gcacgccgcg gatctcctga cgacgctgaa gacgggctgg ctcctcggcg
     7081 cgaagccgcg ccaccagctc tacgcgcagc tgttcggcgt gctcgcaggc gccgctgtcg
     7141 tggtgcccgc gttcctgttg atcatcccgc agccaacgcc cgaccaagcc gcccacggca
     7201 tcacgacggt gctcggaacg caggagtggc cggcgccgtc gagcgtcgtg tgggccggcg
     7261 tgtcggaagc cttctccaat ggcatcggca agatgcatca caccgtcaag gtagcgatcg
     7321 ggatcggctt cgcgatcggc atcgggctca cgatcttcga gaagctcgcg cccaagcacg
     7381 tgaagccttt catcccgtcg gcgaacggca tcgggatcgc gatggtgctg cccggcttca
     7441 actcgatcgc gatgttcacc ggcgctgcga tcgcggagat cctgcggcgc ggccagcgct
     7501 cgagcgcggt catgcccgtc gcgtccggca tcatcgcggg cgagagcttg atgggcgtgc
     7561 tgatcgcagt gctgacgacg atcgtcggcc tcgacctcaa gctttaggcc gcaagagcgc
     7621 gaccgcacaa ccgatgacga gcgcgacctc ggctgcgatc cagatgtact gacgcgtgtc
     7681 tgggctgcca tcgagcgcga agccaacgag ccgcgccgcg ccgatacctg ccatcgccca
     7741 catcacaagc ccggcgaacg cgcgccagcg cggtgaccat cccgggagcc acgcgatgta
     7801 cgcgcccagc ccagcgagtg tgccgcccca actcgcacgc aggttgatca tccgcgtcgg
     7861 tgtgtcgtgt ggctttccca gcgcgtcggc aacggccgtc ggtgagaaca ccagtacacc
     7921 gccgaccagc acgaccgcga ggccgacgat cacctgcaag gtccgcaggt ttcccctagg
     7981 cacccgacga cgatagcaga cgggtcgcaa ccccatgcgt caccgtcgcg ggtcagcaac
     8041 tgggatatac tcgatcgggt gcagcccacc gggcagctga tcgacggcaa gtacgagctc
     8101 gtggcagtcg ctggcaaagg cggcatggcc acggtctaca aggccatgca aaagggcgcc
     8161 gctgggttcc agcgccccgt cgcgatcaag aagatcaagc ccgagtaccg cgcgctcaag
     8221 aactacgtcg acatgttcat cgaagaggcg cgcgtcggca gcgagctcgc gcatccgaac
     8281 atcgtccagg ttcacgactt cgtcgcgcag gacggcagct actacctcgt catggagtgg
     8341 gtcgaaggca tcgacctcgg cctcttcctc aagctgcatc gcgacgcggg cattcatgtc
     8401 gagtggccgc tggttgttgc catcggcatc ggcacgctgc gcggtctcgg cgcggcgcac
     8461 gatcgtcgcg ggccgaacgg cgagccatcg cccgtcattc atcgcgatgt cacgccgcac
     8521 aacgtgctgc tcggcgtgaa cggcgtcgtg aagctgaccg atttcggtct cgcgcgtgcg
     8581 cgcgatcgcg ttgccagcat gacggcgccc ggcaccgtga agggcaagct cagctacctc
     8641 gcgccggagg tgtcgttcgg caaggcgaac acgccgcagt cggatctgtt ctgcgtcggc
     8701 agcgtgttgt gggagacgct ctccggcgag cggttgttcg acggcaagac ggacgtcgag
     8761 atcttcaaga agatccgcgc gtgcaaggtg ccgccgattc aagagcgcag gccggatgtg
     8821 ccgccgtcgc ttgcggccgt cctcgacgtc gcgctcgata tcgatccgaa caatcggttt
     8881 gcgtcggcga ccgcgttcgc gcatgcgctc gcgcaggtca tgaagcaggc cgttggcatc
     8941 gatccgcaga gtgcgctcgg cgcggcggtt cgcgagctgc gtgcgacggg caaggccggc
     9001 acgtcgagcg cggcaacgag cgactacgat ctgtcgaagg cggacatgga cgaagcgccg
     9061 ctcgagctca cgaatccgaa gaagacgaag ccgccgatct gactattcgt tgcgcggggg
     9121 ctcgacggcc ttcgcttgaa acgcgctgcg atcatcgagg cggaacgcgg tgagcgactt
     9181 gccccagacg cagcccgtgt cgagcgcgat gtggtgcggc ccgagatcga ggccgagcgc
     9241 cgcccagtga ccgaatacga ccgtgtgcgt ctgccacgcg gcgttcggga acttgaacca
     9301 tggtgtgaga tccgacggcg cgtctttggg ggcaccgttg aagtcgtcgt cggcgcggcc
     9361 atcggctttg agcatgcgtg cgcgcgtgag gtacgcgagg atcgcacgcc agcgatcgcc
     9421 gccaccgagc gatggcttcc agcgaggagc cgagcccttg agctgcgcga ggaatgctcg
     9481 ccacgtcgga gcgcggagct gcgtttcgat ctcgccggcg cgcgcgcgtg catcgtcgat
     9541 cgtccactcg ggatgcagcc cggcgtggac catcgccacc ctcgcgacgc tcggtgcacc
     9601 tggtgaggtc ggcgcgcgac gcgatccggc gtcgacgtgg agaagcgggc gtgcgcgcag
     9661 ccaatcgatc agctttcccg catcgcgcgc gttcaggatg tcatcgagcg tgtcgcgctt
     9721 cttctcggga gccgcgcccg cgtaacgcgc gaggagatga atgtcgtgat tgccgagcac
     9781 gcacgtgatg acgtcgtcgt gctcgtacgc ccagcgcagg acatcgagcg agcgcggccc
     9841 gcgattgacg agatcgccga cgagccacag tcgatcggac tgcggcgaga agtcgaagag
     9901 tgacagcaac cgctcgaggc tggccatgca gccttggatg tcgccgatga cgtagagcgc
     9961 cactacacct cgatccgcgc ggtgataaag cccgtgcgcg ccgagccgat ctggacgtcg
    10021 agcgtgccgc cgcctttgac gaggtacgtc gcccacgctg cgttcgtcgt gcctcgcgtg
    10081 cccggatacg cgggcatgtt cgcgccggtg tgaaggccat ggccccaacc gtcgaggtgg
    10141 ccgagcgtct gatgcgcctt gctgccttcg acgagcggtc cctttgcgac cgcgtacagc
    10201 ggctcgttga agtcgagcgg cttcgcgctc ggcgcgccat acgtaccgag gtatccctcg
    10261 ttcgtgacct gcacctcgac gcggacgagc ccaccgggca acgcggagcg atcggtgcgc
    10321 gtgatgcgaa ggcgaggggc gagcgcggcg agccgcacga acacgtgagc ctgtgacgcg
    10381 cagagggcgg gcagctcgtg gggcggtgga ttccacattc cgatgcgcgg atcgatgccg
    10441 ccgatctcga cggggccgag ctgcggatgc tcgaacggct tccagccggg gaagcatcgg
    10501 ccttcgttct cgtcgcgatc ccaccacgcg aacttgatga tgtcggcgcg cgagacgcgc
    10561 tcgtagtact ggacgaactt cggcgggcgc tccatgccga gccgcttgaa caggtcccac
    10621 agctcgacga cgtacgcgag cgcgccgcgt tggttgtagc cgtagtcgga gagatcgccg
    10681 cgcagcggtt tgtcgggctc gtagaggaat tcgtcgtggc cagacaccgt cggataaccg
    10741 gtgtgctcgg tcatccactg ctcgatctgg cggaagatcg cgaggtcttc ctggttcatc
    10801 ttcgagtcag gtccgtgacc gagcggccgg atcagcacgc cgccgaacgt gtggtagtcg
    10861 acccacgcga agatctcggg atgcgcgctc gtgaactcga tgatgccgcg cgcctccggc
    10921 tcgcttgccg gaaacgggcc ggcgccgatc tgctcgtgat gcggcgccca gaaccatggg
    10981 aagttgcggt tgagatcgat cgggttgtca ccgaggaaga acggcgaggg aatcgtcttg
    11041 ccgtcgaagt gctcgatgac gccttcggga tagaccttgt agaacggtcc ttcgtcttcg
    11101 atcgtgcgct cgacgagcag gccgggaaac tgcggcgttt cgacgagctc gccgccagga
    11161 tctttgacgc gcatcgcgag cgcgaggccg tcgccgttca tgtccttcag gatccagcgc
    11221 ggttgcccct gatggacgcg gctgtcgcgc ggcacggagc gcacggtacg gccggtcttc
    11281 agcacgcatt cggcgccgtc gggcgagacg cgcggcacca cgtagaacag cacctcgcgc
    11341 atgcgatcga tgatcggctg cggaaggtcg aggctgtcgg gctcgaggtg cgcgcgcagt
    11401 gcatcctcgg cgatcgagag cgcgacgctc gaacctgcga gctccgcggc gtgcacgttg
    11461 ccgtccgccc acaccgcggg gcggatccgg tccggctcgg gaccgatcgt gacgagccag
    11521 acatcgcgac cctcgggagt cttggccagc gacgtgagcc gaacgagaga cgggtaggca
    11581 tctgcccacg cctttagctg gccgttcagc tgggcgtcat cgaggtagga ctggcgaaaa
    11641 ccacgggata gatcggcaag tgcgcgcgcc atgggtgatg aagcttacac cgtgaagaag
    11701 tcggcaatgc cagacgtcta ctcggtgttc atactagcga gatgcccatg ctgagcttcg
    11761 attcacttcc ccaagcgcgc gtggttcgca agggtggcca gcacttcgcc ccgccgcccg
    11821 ctccactgac ctacgagcag agcgcgatgc gcgagaagat gcacgacgcg ctcgcgctga
    11881 tgatcggcct gtggccgttg accgcgatcg ttctgctctg cgtcggcctc atcatcatgg
    11941 ccggcaacaa cggcgctccg tagcgtcgat cattctgcgc accgccttcg gcgctgctgc
    12001 gcggtccagc aaagcgggac cttgctactc gagaggcatc gcgcgcagag ccttcggcag
    12061 atcgagcagg ctctccgtag gcgagcccac caacatcgcc gggctcatgt tcgtgagtcc
    12121 ccacggctcg atggttccgg ccgtcacggc gaatgcgccg ccgtcgatgc ggccgacgac
    12181 accgaagcca gagcgcatcg gcacgcgcgc ggtttcgatg acgaggtcgg gcaggtggct
    12241 gacgtcgatc tcgacgaggc cgcgctcgca cgtgagcacg agccgatact ggagcacccg
    12301 ccgctcgacg agcaggacgt tgcccatctg gctcgtgaag acttcctgcc agccttccgt
    12361 cggcgcggcg tgctcgcgca gcgattgcca gtcgagatga aacaggcgcg catcgtcgat
    12421 cttgcgagcg aggattccgg tcgtcgtgac gacacaatcg agatagccga gctcgtgcag
    12481 cgcgacgacg cgcgagcgtt cgttgatcgt ccaccacgat ccgggcacgt gcggcgcgca
    12541 gccgaggaag acgttcttcg cggagctgcc cttgagccac tcgctaatcg cggtcgcgac
    12601 ctcggaggcg gagccggtct cggcgaggcg gcggtcgccg ttccacagca cggtgtcacc
    12661 ggggaggcgg tgcacggcgt cgaagttgaa gtagcggcct tcccacggcg ggacgagcga
    12721 gagcttgtcg ccgaccttga tgctcgcgac cgcgtcgagg tcttcgggca ccgcgatgta
    12781 cgtctcgcct cgcggatcga gcgcgatgaa cagcaggccg cgtggcgtca ggccctgctt
    12841 gcgcagctcc ttgagaccga ggatgaccgc cgccgggtcg cgataatgat agacgcgcgt
    12901 ggcgaacatc gcggcgactc tacaacagca cgcccttgag caggatgtgg gcgatcgtga
    12961 agtagatgac gatgcctgtc acgtcgacga gcgtcgcgac gaacggcgca gatgcgctgg
    13021 cagggtcggc gccgaccttc ctgaggagga atggcagcat cgagcctgcg agcgtccccg
    13081 acaagacgca cccgagcacg gcccctccga cgacggctgc gacgaggaag taatgatcgc
    13141 cgtagacgcc gaacgtgccc cagatgagca cacgaacgag cgcgaccatg ccgaggaccg
    13201 cgccgagcgc gaggcccatc gcgagctccc gtcgcatcac gagccaccag tcagcgctgc
    13261 ggatctcgcc gagcgccatc gcgcgaatga cgagcgtcga cgcttgcgag cccgagttgc
    13321 cacccgccga gacgatcagc gggatgaaca gcgtcagcac gatcgcggcc ttcagctgat
    13381 cttcgaagaa gcccatcgcc gtcgtcgtga gcatctcgcc gacgagcagc agcacgagcc
    13441 agcgaccgcg cttgctgagc atgtcgcgcg
//
LOCUS       DATLBZ010000004         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1494867, whole genome shotgun sequence.
ACCESSION   DATLBZ010000004 DATLBZ010000000
VERSION     DATLBZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494867"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..597)
                     /gene="moaB"
                     /locus_tag="VMZ53_00135"
     CDS             complement(73..597)
                     /gene="moaB"
                     /locus_tag="VMZ53_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HUS26873.1"
                     /translation="MEREFIPVNVAVLTVSDTRTVENDTSGAYIADRLAKEGHQIVGR
                     AIVKDSETLIRAQLAAWIADPSIEVIIATGGTGVTPRDVTPEALAPLITKHIPGFGEL
                     FRQISFAEIGTATIQSRADAALCGQTYVFLLPGSTNGVKTGVDKILVPQLDHRIKPCN
                     FVMLLPRIKNEKTS"
     gene            687..1067
                     /locus_tag="VMZ53_00140"
     CDS             687..1067
                     /locus_tag="VMZ53_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26874.1"
                     /translation="MKETTKTESLTRSSSDRAGFVPGVTHLALDVVDRGQSTVIAVLQ
                     DARIELRTLVDHGIEFAEKGTAGLFRFARKLTQRVDEGVGETLTSTERLIGGAVKSAR
                     ETAHAAQETANSAIAGVTGATAKA"
     gene            complement(1292..>2231)
                     /locus_tag="VMZ53_00145"
     CDS             complement(1292..>2231)
                     /locus_tag="VMZ53_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUS26875.1"
                     /translation="GGNEHAVLHLLYARFWHKVLFDAGLVSTKEPFQRLFHQGMIHKT
                     SFRDASGKYHHDHEVEQRDGTWFVKGTDTPVETKLDKMSKSRYNVVNPDDMCGEFGAD
                     AMRMYELFMGPLEDGVEWETAGVQGTRRFLDRVWRLLIDPETDTLTSKVSADAATDNK
                     DLERALHAAIKKVTGAVADLRFNTAISEMMVFVNEATKATTVPREWFESFVKILSPFA
                     PHLAEEIWQRMGHTTSITYAPWPAFDEAKLARDTMMIAIQVTGKLRGQIEVPTDATEA
                     TILAAAKADDKVQSFIAGKPIKREIYVKGRLVNIVV"
BASE COUNT          355 a          734 c          706 g          436 t
ORIGIN      
        1 cgaagccgcg cgcatggctc gcatggatcg gccgtacctg ctcacgctcc tgcgcaagca
       61 cggcctgcgc agctagctcg tcttctcgtt cttgatccgc ggcagcagca tcacgaagtt
      121 gcacggcttg atgcggtgat ccagctgcgg aacgaggatc ttgtcgacac cggtcttcac
      181 gccgttcgtc gatccgggca ggaggaacac gtacgtctgg ccgcacagcg ctgcatccgc
      241 gcgtgattgg atcgtcgccg tcccgatctc cgcgaacgag atctggcgga acagctcgcc
      301 gaagccgggg atgtgcttcg tgatgagcgg cgcgagcgct tcgggcgtga catcgcgcgg
      361 ggtgacgccg gtgccgccgg tcgcgatgat gacctcgatg ctcgggtcgg cgatccacgc
      421 ggcgagctgc gcgcggatga gcgtctcgct gtctttgacg atcgcgcggc ccacaatttg
      481 gtgaccctct ttggcgagcc ggtcggcgat gtatgcgccc gacgtatcgt tctcgaccgt
      541 ccgcgtatcg gagaccgtca gcaccgccac gttcaccgga atgaactcgc gttccatgta
      601 cttagcgtac tccgtgacgc gacgcgttag cagctgtcag caggcgaaac ctggtgaatg
      661 acgcattgcg tcttatggtg ctcggcatga aagagaccac caagaccgag tcgctgaccc
      721 gctcgagctc cgaccgcgcc ggcttcgttc cgggcgtcac ccatctcgcg ctcgatgttg
      781 tcgatcgcgg ccagagcacg gtgatcgcgg ttctccagga cgcgcgcatc gagctccgca
      841 ccctggtcga tcacggcatc gagttcgcgg agaagggcac cgcgggcctg ttccgcttcg
      901 cgcgcaagct cacgcagcgc gtggacgaag gcgtcggcga gacgctcacg agcaccgagc
      961 gcctcatcgg cggcgcggtg aagtcggcgc gcgagaccgc acacgcggcg caggaaactg
     1021 cgaacagcgc gatcgcgggc gtcaccggcg caaccgcgaa ggcgtaatca gcgattcttc
     1081 cgcgagctcg tcgaacaaca tccgagcccg caggaggaca tcgccttctc gcgagggaca
     1141 tggctcgcgc gcgagtcgca ctcgcgaatc ctcttctcgc cctttgcagg gcgcacatag
     1201 cttcgttcgc ttaacgactt tcggcgggcg caggtgacgg tagagattcc ccccatttcc
     1261 cgttgcctgc gttcgccccc ttttggggcc tctacacgac gatgttgacg aggcggccct
     1321 tgacgtagat ctcgcgcttg atcggcttgc ccgcgatgaa gctctgcacc ttgtcgtcgg
     1381 ctttcgccgc ggcgaggatc gtggcttcgg tcgcgtcggt cggcacttcg atctgaccgc
     1441 gcagctttcc cgtaacttgg atcgcgatca tcatcgtgtc gcgcgcgagc tttgcctcgt
     1501 cgaatgcagg ccacggcgcg tacgtgatcg acgtcgtatg gcccatgcgc tgccagatct
     1561 cttcggcgag atgcggcgcg aacggcgaca ggatcttcac gaagctctcg aaccactcgc
     1621 gcggcacggt ggtcgctttc gtcgcttcgt tgacgaagac catcatttcc gagatcgccg
     1681 tgttgaagcg caggtcggcg acggcgccgg tgaccttctt gatcgcggcg tgcagcgcgc
     1741 gctcgagatc tttattgtcc gtcgctgcat ccgccgagac cttcgacgtc agggtgtccg
     1801 tctcgggatc gatgagcagt cgccacacgc gatcgaggaa gcgacgcgtg ccctgcacgc
     1861 ccgccgtctc ccactcgacg ccgtcttcga gcggtcccat gaacagctcg tacatgcgca
     1921 tcgcgtcggc gccgaactcg ccgcacatgt cgtcgggatt gacgacgttg tagcggctct
     1981 tcgacatctt atcgagcttc gtctcgacgg gcgtgtccgt ccctttgacg aaccacgtgc
     2041 cgtcgcgctg ctcgacctcg tggtcgtggt gatatttgcc actcgcgtcg cggaacgacg
     2101 tcttgtggat cattccctgg tggaacagcc gctggaacgg ttccttcgtc gacacgagcc
     2161 cggcgtcgaa cagcaccttg tgccagaacc gcgcgtagag caggtgcagc accgcgtgct
     2221 cgttgccgcc g
//
LOCUS       DATLBZ010000005         6564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4476784, whole genome shotgun sequence.
ACCESSION   DATLBZ010000005 DATLBZ010000000
VERSION     DATLBZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4476784"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VMZ53_00150"
     CDS             <1..344
                     /locus_tag="VMZ53_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS26876.1"
                     /translation="VDDQPTLARAIKRMLNEHDVTTVGNARDALTRIEEGERFDVILS
                     DLMMPELSGMDLHAAITQIAPDQVDKMVFMTGGAFTGWARDFFEQVPNPTIEKPFDKA
                     ALLAIIETFLR"
     gene            complement(346..1440)
                     /locus_tag="VMZ53_00155"
     CDS             complement(346..1440)
                     /locus_tag="VMZ53_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26877.1"
                     /translation="MRSLMCVMGILGAARVADASPCSVNIARAPDGVRDVVAKWVLAE
                     HDCGPALEVRIVETPDGLYVLARDEAGRTHERVVPDAQTAGVLVASWAAASGGESSAV
                     RAEMSIELSWAAPATSAVIAPGTTTVLAPGAARVSARDSMPVPAPPPGPAPIVMPVRV
                     VAPSDTLLVDAAVVPAARTKVPAKWMSLGGTLGAGGAGAYGVRGEADLAARGAWRFGV
                     TGSIAHVDVNPDLGRYAGYLDLLDMQAAVYATHSVDVGNWQVRAGAGVGLVLTRGNGT
                     LFDRADVNEKAALWPQISDLSAIGEVAVSFSHGLGWQWPGWGFQLGPVVTVHDQSMKL
                     KMGEANLPYDSDYVRSGVELMFYGGIRHEL"
     gene            complement(1499..3283)
                     /locus_tag="VMZ53_00160"
     CDS             complement(1499..3283)
                     /locus_tag="VMZ53_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HUS26878.1"
                     /translation="MTAVVTVQDVEKHFGTRRVLNGVSFAVHDRDRIGLIGANGSGKS
                     TLLKIMVGDLTPDEGAVTWQRHLRLEYVSQEPALRVEHSIAQALAEKRDEVPEHQVHT
                     VAAALQLPPLTKIVGELSLGERRRVALAHALLGKPDVLALDEPTNHLDARTVEWLETR
                     LAAWQGSLIVVTHDRYFLDRVSTRIIEIDRGKAYTYEGNYEEFLLAQADRLSVEADQE
                     HERSMFVRREIEWVRRQPKARTTKAKARLDRFDDAVAAAPTIDDKRGPTLRLQLPAGP
                     RLGGNIVELKNVAKSLGGKKLFENLTLVMKPGDRIGIVGVNGAGKTTLIRTILGLEKP
                     DRGEVIAGINTKPAYFEQGRSELRDELTVLEEVGDGYDWVDLPDGRVHVRGFLRMMAF
                     PDSIADTKIGQLSGGERNRVQLARLLRRGGNLLVLDEPTNDLDLPTLGALEDGLISFP
                     GCALIVSHDRWFLDRVATGILAFEGNGEVTFYEGSYSFYAERRASRSATTDERDAKDK
                     PGKPRVKQVAPRKLTFKEKAELAAIEDTIAAAEKKVSDLEATLSDPTVFKDRPTEVQT
                     LVAELDGARAEVERLFARWQELDAIPKE"
     gene            3350..5596
                     /locus_tag="VMZ53_00165"
     CDS             3350..5596
                     /locus_tag="VMZ53_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUS26879.1"
                     /translation="MATATGALRVLFVDDDELQLRAARRSVRGRLNIELVTMTSALEA
                     LETLHLTLPDVVILDVFMPELDGVEACRRIKASPYTRDIEVLLASMDMTPELERVARS
                     AGAARGISKPFDLTNLVVAPKHPARTVDAPVVMTKMTAATAAQAAPRGADLLVSMLAQ
                     AGVDVVFGLPGGAISPVHDALFDQSIRVITTKHEANAMFAAAGYAHSTGKLGVVAVTS
                     GPGALNAMTGLASAWCDGLPLLLLVGDVPRAAQGRGVLQDSSAHGLQIVEMSRHITKL
                     AAEVSSSSSLPHMIRRAIATAMSGRKGPVVLTLPIDVMMQPCVAPRVAGSMTMGAHVA
                     GETLDEIAELLLGAERPLILAGSGIRGGAAPEHLRHVAERFSIPVATTPKGKGVFPES
                     HELSLGVLGLGGHRSARQYIDLGVDVVLAIGTSLGDVATDGFAPSLQASRALIHVDID
                     ARQLGKSYSPTHAVVASAAELLGGLADRRTSFKPLKRMPMGTTPPPFAGTATASEGAA
                     ASYVAPSPPPQPGHPAAGVVRHALPSSNKPDRIASHDAIRDIQRCLPKDTIYTVDSGE
                     HFLFAVQYLEIDHADSFVVMTGLGSMGQSIGAAIGAQLAHPNRTVAAICGDGCFAMNA
                     FEIATAVQEQIPLRIFVFNDEALGMVENGHEMVYGRRPRYSTGPLDILSIARGLGATT
                     ARVDQHGQLDADIFNTPGPVVIEMRIDAAVKIPKVDRVAVMSTKPTLKALPAAAQTQP
                     LPRLRIVN"
     gene            complement(5589..>6564)
                     /locus_tag="VMZ53_00170"
     CDS             complement(5589..>6564)
                     /locus_tag="VMZ53_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HUS26880.1"
                     /translation="MMNRDPPDHTWLRRLVNKALNPRRIAQLDATVARHAREIVESVR
                     ERGACDFASDVAEEMPLFMISEILGVPLEDRRALYSLTMRMFTTEVIDPAEALRDKMA
                     AVAEMRSYAAGLGQRKRANPADDLTSELVSVELDGRRLTEDEFQAFFMLLFNAGTDTT
                     RSLLCYGLDLLLDRPEVVERLADDRSLIPVAIEEMLRYEPPVIQIRRTTTRTVELGGR
                     QIDEGAKVVVFFPSANRDETVFPDPDRFDIDRQQNDHIAFGFGTHHCLGAPLARLESK
                     HIFSEVLAQLADIERAAPTVPIRTNFVRGIKHLQIRFRARANHSAATS"
BASE COUNT         1108 a         2276 c         2047 g         1133 t
ORIGIN      
        1 tcgtggacga tcagccgacg ctcgcgcgcg ccatcaagcg catgctgaac gagcacgacg
       61 tcaccaccgt cgggaatgcc cgcgacgcgc tcacgcggat cgaagagggc gagcgcttcg
      121 acgtgatctt gagcgacctg atgatgcccg agctctccgg catggatttg cacgcggcca
      181 tcacgcagat cgcgcccgac caagtcgaca agatggtgtt catgaccggc ggcgcattca
      241 ccggctgggc gcgcgacttc ttcgagcagg tgccaaaccc gacgatcgag aagccgttcg
      301 acaaagcagc gctgctcgcg atcatcgaga cgttcctgcg ctgactcaga gctcgtggcg
      361 aatgccgccg tagaacatga gctcgacgcc gctgcggacg tagtccgagt cgtagggcag
      421 gttggcttcg cccatcttca gtttcatcga ctggtcgtga acggtgacga cgggcccgag
      481 ctgaaatccc catcccggcc actgccagcc aaggccgtgc gagaagctaa ccgccacctc
      541 gccgatcgcc gacagatccg aaatctgcgg ccacagcgca gccttctcgt tcacgtccgc
      601 gcgatcgaag agcgtgccgt tgcccctcgt gagcacgaga cccacgccgg cacccgcacg
      661 cacctgccag ttgccgacgt cgacgctatg cgtggcgtac accgccgcct gcatatcgag
      721 gagatcgagg taccccgcat aacgcccgag gtcgggattg acgtcgacgt gcgcgatcga
      781 tccggtcacg ccaaaccgcc aagcgccgcg cgcggcaaga tccgcctcgc cgcgcacgcc
      841 atacgcaccc gcgccacctg caccgagcgt tccgccgagc gacatccact tcgccgggac
      901 cttcgtccga gccgcgggaa cgacggctgc gtcgacgagc aacgtgtcac tcggcgctac
      961 cacgcgcacc ggcatgacaa taggggctgg gccaggcggt ggcgcgggga cgggcatcga
     1021 atcgcgagct gagacgcgcg cagcaccagg cgcgagcacg gtagtcgttc cgggcgcgat
     1081 gaccgcactc gtcgccggcg ccgcccacga gagctcgata gacatctcgg cgcgaacagc
     1141 gctggactcg ccacccgatg ccgccgccca gctcgcaacg agcacgcccg ccgtctgcgc
     1201 atcaggaacg acgcgctcgt gagtacggcc cgcttcgtcg cgcgcgagca cgtagagccc
     1261 gtccggcgtc tcgacgatgc gcacttcgag cgccggcccg cagtcgtgct cggcgagcac
     1321 ccacttcgcg acgacatcgc gaacaccgtc gggcgcacgc gcgatgttga cgctgcacgg
     1381 gctcgcatcg gcaacgcgag cggcaccgag gatccccatc acgcacatga ggctacgcat
     1441 gccggtatgg ttgccgcacc agcaggtttt catcgctggc aacgaccgtt tttcgcgcct
     1501 attccttcgg aatcgcgtcg agctcttgcc agcgcgcgaa caggcgctcg acctcggcgc
     1561 gcgcgccgtc gagctcggcg acgagcgtct ggacctcggt cggtcgatct ttgaagacgg
     1621 tcgggtccga cagcgtcgct tcgaggtcgc tgactttttt ctcggcggca gcgatcgtgt
     1681 cttcgatcgc ggcgagctcc gccttctctt tgaacgtcag cttgcgcggc gcgacttgtt
     1741 tgacgcgcgg cttgccgggc ttgtccttcg cgtcgcgctc gtcggtagtc gcggacctcg
     1801 acgctcgacg ctcggcgtag aacgaatagc tgccttcgta gaacgtgacc tcgccgttgc
     1861 cctcgaatgc gaggatgccg gtcgcgacgc gatcgagaaa ccagcgatcg tgcgagacga
     1921 tcagcgcgca gcccgggaac gagatgaggc cgtcttcgag cgcgccgagc gtcggcaggt
     1981 cgaggtcgtt tgtcggctcg tcgagcacga ggaggttgcc gcctcggcgc aagagacgcg
     2041 cgagctgcac gcggtttcgt tcaccgcccg acagctggcc gatcttggtg tcggcgatcg
     2101 aatcggggaa cgccatcatg cgcaaaaagc cgcgcacatg gacgcgacca tcgggcaggt
     2161 cgacccagtc gtagccgtcg ccgacttctt cgaggacggt cagctcgtct cgcagctcgc
     2221 tgcgaccttg ctcgaagtac gccggcttcg tgttgatgcc cgcaatcacc tcgccgcggt
     2281 ccggcttctc gaggccgagg atcgtcctga tcagcgtggt cttgcctgcg ccgttgacgc
     2341 caacgatgcc gatgcgatcg ccaggcttca tcacgagcgt caggttctcg aacagcttct
     2401 tgccgccgag cgacttcgcg acgttcttca gctcgacgat gttgccgccg aggcgcggcc
     2461 ctgcgggcag ctggagacga agggtggggc cacgtttgtc gtcgatggtc ggcgccgcag
     2521 cgaccgcgtc gtcgaagcga tcgaggcgag ctttcgcttt cgtcgtgcgc gcctttggct
     2581 ggcgacgcac ccactcgatc tcgcggcgca cgaacatcga tcgctcgtgc tcctgatcgg
     2641 cttcgaccga gaggcggtct gcttgcgcga gcagaaactc ctcgtagttg ccttcgtacg
     2701 tgtacgcctt gccgcgatcg atctcgatga tgcgcgtcga cacgcgatcg agaaaatagc
     2761 gatcgtgcgt gacgacgatc agcgagcctt gccacgccgc cagccgcgtc tcgagccact
     2821 cgaccgtgcg tgcatcgaga tggttcgtcg gctcgtcgag cgcgagcacg tcgggcttgc
     2881 cgagcagcgc atgcgcgagc gccacgcgtc gtcgctcacc gagcgacagc tcgccgacga
     2941 tcttcgtcag cggcggcagc tggagggcgg ccgccacggt gtggacctgg tgctccggta
     3001 cttcgtcacg tttttccgcg agggcctgcg cgatcgagtg ctcgacccgg agcgccggct
     3061 cctgcgacac atactcgagt cgcagatggc gctgccaggt gacggcgccc tcgtcgggcg
     3121 taaggtcgcc caccatgatc ttgagtaacg tcgacttgcc ggagccattg gcgccgatga
     3181 gcccgattcg atcgcggtcg tgaacggcga acgagacccc gttcaagacg cggcgcgtcc
     3241 cgaagtgctt ctcgacatcc tggaccgtca cgactgccgt cacagccgga cgtatacgac
     3301 gaccgattgc cagttcccac tgctgtggcc agaggtataa ggcccctcaa tggccaccgc
     3361 gaccggcgct ctacgcgtgc tcttcgtcga cgatgatgag ctccagctcc gagcagctcg
     3421 ccggtccgtc cgcggtcgcc tcaacatcga gcttgtcacg atgacgagcg cgctcgaagc
     3481 gctcgagacg ctgcacctga cgctgcccga cgtcgtcatc ctcgacgtgt tcatgcccga
     3541 gctcgacggc gtcgaggcat gccgtcggat caaggcttcg ccgtacaccc gcgatattga
     3601 agtccttctc gcgagcatgg acatgacgcc ggagctcgag cgcgttgccc ggtcggccgg
     3661 cgccgctcgg gggatctcga agcccttcga cctcacaaac ctggtcgttg cccctaaaca
     3721 tcccgcgcgg acggtcgatg cgccggttgt gatgacaaag atgacagccg ccacagcagc
     3781 gcaagctgcc ccacgtggcg ctgacctcct cgtttcgatg ctcgcgcaag ccggtgtgga
     3841 tgtcgtgttc ggcctcccgg gtggcgccat ctcgccggtg cacgacgcgc tgttcgatca
     3901 gtcgattcgc gtcatcacga cgaagcacga agcgaacgcg atgttcgccg ccgccggtta
     3961 cgcccactcg acgggcaagc tcggcgtcgt cgccgtcacc tccggtcccg gcgcgctgaa
     4021 cgcgatgacc ggtctcgcct ccgcatggtg cgatggcctg ccgctgctcc tcctcgttgg
     4081 cgacgtgccg cgcgccgcgc aaggccgcgg tgtcctccaa gactcgtccg cgcacggtct
     4141 tcagatcgtc gagatgtcgc gacacatcac gaagctcgcc gccgaagtct cgagctcctc
     4201 gtcgctgccg cacatgattc gccgcgcgat tgccaccgcc atgagcggcc gcaaaggccc
     4261 cgtcgtcctc acgcttccga tcgacgtcat gatgcagccg tgcgtcgcac cgcgcgttgc
     4321 cggttcgatg acgatgggcg cgcatgtcgc gggcgaaacg ctcgacgaga tcgccgagct
     4381 cctcctcggt gccgagcgcc cgctcatcct cgcaggctcg ggcatccgcg gcggcgcggc
     4441 gccggaacat ctccgccacg tcgccgagcg cttcagcatc cctgtcgcga cgacacccaa
     4501 aggcaaaggc gtattcccgg agtcgcacga gctctcgctc ggcgtcctcg gcctcggcgg
     4561 ccatcgctct gcgcgccagt acatcgacct cggcgtcgac gtggtcctcg ccatcggcac
     4621 gtcgctcggc gatgtcgcca cggacggctt cgcgccatcc cttcaagcga gccgcgccct
     4681 catccacgtc gatatcgacg cgcgccagct cggcaagagc tactcgccga cgcacgcggt
     4741 tgtcgcctcc gccgcggagc tcctcggtgg tctcgccgat cgccggacga gcttcaagcc
     4801 gctcaagcgc atgccgatgg gcaccacgcc gccgccgttc gccggcacgg ccaccgcttc
     4861 cgaaggcgca gccgcctcgt acgtcgcccc ttcgccgccg ccgcaacccg gccatcccgc
     4921 cgccggcgtc gttcgccacg cgctgccctc cagcaacaag cccgatcgca tcgcctcgca
     4981 cgacgcgatc cgcgacatcc agcgctgcct gcccaaagac acgatctaca cggtcgactc
     5041 gggcgagcac ttcctattcg ccgtccaata cctcgagatc gatcacgcag actcgttcgt
     5101 cgtgatgacc ggcctcggct cgatgggcca atcgatcggc gccgcgatcg gcgcgcagct
     5161 cgcacatccg aaccgcaccg tcgccgccat ctgtggtgac ggctgcttcg cgatgaacgc
     5221 gttcgagatc gcgaccgcgg tccaagagca aatcccgctg cgcatcttcg tgttcaacga
     5281 cgaggcgctc ggcatggtcg agaacggcca cgagatggtg tacggccgtc gcccgcgcta
     5341 ctcgacgggc ccgctcgaca tcctctcgat cgctcgcggc ctcggcgcca cgaccgcacg
     5401 cgtcgatcaa cacggccaac tcgatgccga catcttcaac acgccgggcc ccgtcgtgat
     5461 cgagatgcgc atcgacgccg cggtgaagat cccgaaagtc gatcgcgtgg ccgtcatgtc
     5521 gacgaagccg acgctcaaag cgttgccggc cgccgcgcaa acgcagccgc tgccgcgcct
     5581 ccgcatcgtc aactagtcgc cgcggagtgg ttggctcggg cgcggaatcg gatctggagg
     5641 tgcttgatgc cacggacaaa gttcgtccgg atcggaaccg tgggcgcggc gcgttcgata
     5701 tcggcgagtt gggccagcac ctcggaaaag atgtgtttgc tttccagacg cgcaagaggc
     5761 gctccgaggc aatggtgagt gccgaatccg aacgcgatgt gatcgttttg ttggcgatcg
     5821 atatcgaagc ggtcgggatc tggaaacacc gtttcgtcgc gattcgccga agggaagaac
     5881 acaaccacct tcgctccctc atcgatttgc cgaccaccca gctcgaccgt gcgtgtggtc
     5941 gtgcggcgga tctggatcac cggtggctcg taacgaagca tttcttcgat cgcgacgggt
     6001 atgagggagc gatcatctgc cagtcgttcg acgacctcag gtcggtcaag caacaagtcc
     6061 aggccatagc agagcaagct tcgcgtcgta tccgttccgg cattgaacag gagcatgaag
     6121 aacgcctgaa actcatcttc agtaaggcgt cgtccgtcga gctcgacgga aacgagctcg
     6181 ctcgtgaggt cgtcggcagg attcgccctc ttcctttgcc cgaggcccgc cgcgtaactg
     6241 cgcatctcgg cgacggcagc cattttgtcc ctcagtgcct cggcaggatc gatgacctcc
     6301 gttgtgaaca ttcgcatcgt gagcgaataa agcgctcgcc gatcctccag ggggacgccg
     6361 aggatctcgc tgatcataaa gagcggcatc tcttccgcta cgtcactcgc gaaatcgcaa
     6421 gcgccacgct cgcgaacgct ctcgacaatc tcgcgggcat gacgcgcgac agtcgcgtcg
     6481 agttgcgcga ttcgtcgcgg attgagtgct ttgttgacga gccgtcgcag ccaggtgtga
     6541 tcgggtggat cgcgattcat catg
//
LOCUS       DATLBZ010000006         8086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2984389, whole genome shotgun sequence.
ACCESSION   DATLBZ010000006 DATLBZ010000000
VERSION     DATLBZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2984389"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VMZ53_00175"
     CDS             <1..672
                     /locus_tag="VMZ53_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26881.1"
                     /translation="WANDKDVWKLSVETLSTKNALDIQVSAIEGVALELEIADGVGNT
                     LATRKAPKGAPLIVKSLMPVIPEGAPPFHYVTVKGSASNPETSYTLHATARPVGTDEE
                     VEPNDLVDKPAEMNPERTKVYATWTPGDVDCFALQPAQVERTVDITVDTPAEIDLAVE
                     LLAEGKAIGSANAGKKGVVEKLSVKVPPNVKPVIRVKNPDATATAEAKYELSVIEATT
                     GDNVP"
     gene            669..1136
                     /locus_tag="VMZ53_00180"
     CDS             669..1136
                     /locus_tag="VMZ53_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26882.1"
                     /translation="MSWEPDTLQHYLQTQRGFGRVFCDWRERSRQGEPEPTELDEYVA
                     SLFGDVLDDQWARGGYRQVSAREARHQLAFWLEKNLEEGAAPAGSMLESAAIEPFFKL
                     FGAGTSYFTNASMQFPAHGTSSAFMTKTLHDYDAGVIAVDDVRIGLVWFADDE"
     gene            1141..1704
                     /locus_tag="VMZ53_00185"
     CDS             1141..1704
                     /locus_tag="VMZ53_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26883.1"
                     /translation="MSYDIIRLFALDWTATVEALRTQGDDFFWLTRTANGDDIDFSRI
                     PFVAEFEGALASMPGWRDPTAGEIYDNLRQHLAADARERCDACFSRLFWEGNSPLTLR
                     GLRLPEGAPPAHIWNAVSPSSVRDVLGWIQRIPWPSLHAVADRYTGWENRYLPTIDDF
                     ESQVGCHEELLREAMKRNAGVLSLISA"
     gene            1754..3130
                     /locus_tag="VMZ53_00190"
     CDS             1754..3130
                     /locus_tag="VMZ53_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS26884.1"
                     /translation="MGSGDTGRPHAAGAQAGLVGDVIAASGGEPSPRESLWMRRAGGP
                     RILVLLALTNLVAYAVRNALFGVYPDLRAEFHVNDQTLGFLATVFLIPHAVATLPFGW
                     AGDRYDRRRVIAFGIMLSAVASVAGAASRDMWTLGISRAAVGLGTAAVVPVANSILGQ
                     LYEGPRKASRIALFNLGILVGGLVGFGAGIFVGYPAVVVVLAAPMVVLALLVLAMPIP
                     APVAARGPMPRAQEHVGLLQYLFRLAKAFFVDGKMLLRIRTLRWLIVSATAMAFAAGG
                     FNAWLLDFLERDKQMTRASATTLISVAMIGAVAGIVVGGRLADRLRTRMMTGRLWTIA
                     LGMLFAIPCTAICIEIDPGVGLYVAGTLNFFFFSWYHAPIAATVDDLAPPVLAVSAQG
                     LVIFTMHLFGTASASYVVGIVSDHSSLYTAMWVPAGALVLAGLTALCAIPSFAADHKA
                     SRANPAAS"
     gene            complement(3111..3863)
                     /gene="map"
                     /locus_tag="VMZ53_00195"
     CDS             complement(3111..3863)
                     /gene="map"
                     /locus_tag="VMZ53_00195"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HUS26885.1"
                     /translation="MAITLLSRDERDRMRRAGRAAAATLAYVGARLVPGMTTAEIDRL
                     VRAHTDALGGRPSQLGYHGFPAAVCVSRNDIVCHGVPSDDVLLVDGDIVNIDVTTELD
                     GYHGDTSATFIVGDAPPHARALVELARGARDAGIAAAVLGARLGDIGTAISRFVGTAG
                     SVVETYGGHGIGRAMHMDPHVAHVGRPHFGHRLREGMAFTIEPMINAGTSATRVDADG
                     WTVRTVDGSLSAQFEHTILIGPDGPEITTLLD"
     gene            3949..4212
                     /locus_tag="VMZ53_00200"
     CDS             3949..4212
                     /locus_tag="VMZ53_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUS26886.1"
                     /translation="MSLQQLQYFVAVAEEQHVTRAAERLHISQPPLSRQIQALEDELG
                     QPLFERRGRGIVLTEFGRYFAAKAAAILDHVDSVLADTKRWSS"
     gene            complement(4209..5654)
                     /locus_tag="VMZ53_00205"
     CDS             complement(4209..5654)
                     /locus_tag="VMZ53_00205"
                     /inference="COORDINATES: protein motif:HMM:NF017396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070008 - serine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HUS26887.1"
                     /translation="MQRLLATLVLAAGCGAQIEDDGPDGPDQPEPPVAAVCDEAELPA
                     ELSALANVTSSREVACGQYVAAPARCFDVTFRQPISATNPATYDQHLLLTHRGCNRPM
                     VVADWGYSSFGFFDDELATLFHANTLWLEHRYQGESVPAQAAWDWSALTIENGANDTH
                     RVIEQFKTIYGANWVSTGASKGGITAAYHYYFFPHDLDGSIPYVAPASRARVDNEYQS
                     YITQHMTSSCAQKIRDTQVAALTTRHNAMITRLTASAGAGAEESYLQSMAGYLDWSFW
                     QYYGNTSCAAVPTSTTTDDNFFAFLRQASGYPERLPATDARSDGALSYEWLTEQGFAQ
                     QLSPTVASYVTDPSARMNMEDAFKSQYPDIALPAYNGQVTRDVRTWAKSADNLLLIYG
                     ELDPWSGGAMDPPTKPSSRRYFVPGANHGAQIAALPTNDRSNALTLASLYFGEQADVT
                     ALPRAIAANAHRAELLARAAQYEAVLVNGLR"
     gene            5772..6422
                     /locus_tag="VMZ53_00210"
     CDS             5772..6422
                     /locus_tag="VMZ53_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26888.1"
                     /translation="MRYALCLCLLVVAASCRPAKGPSQTLDKYGRALKNHDFSSAYDF
                     MSSSFRGKVSREDYVRMMRDNPREVDETADRLRGKHGSLEVSAEFEYGLGDQMRLVQE
                     GGRWKIASNPVAFYDQSSPKGALRSFIRAYRLERWDIMLRFVPNQYREKMDGAKLKTQ
                     FLGPSKEQVETLMNTLEANVDEPITERGNNARMSYGDRFEVKFLKEDGVWKLQDLD"
     gene            6822..6998
                     /locus_tag="VMZ53_00215"
     CDS             6822..6998
                     /locus_tag="VMZ53_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26889.1"
                     /translation="MLDPWIIEEILKKEEERRRESERSRVELPIERERDTGPRTVVTP
                     PREESERGVMIIDI"
     gene            7088..>8086
                     /locus_tag="VMZ53_00220"
     CDS             7088..>8086
                     /locus_tag="VMZ53_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26890.1"
                     /translation="MASRNGNTGGDQRRADSTPAATSRAKRASNPPPAQGARGSAADA
                     GKRELESGERPRAKRDTGGPSESSRMARFATGESSSGLRRAGTANPRPRSNTSGGGRA
                     GSESAIVVPNRRWIGHTFTIALGIIGWLIVLVLPLTGLVPVDEATPALPIMMFVLVIL
                     VARALAFRLTEGSVLSLDSAYYVAAALCVGTAEAGRLVALALTLDASARLSSKRRRKV
                     DLDGWWAELGYVLYFGGMSGGLLVVCGWVFGADSVVGQMPHEPVEITMRVVAIAGMLL
                     VTHYAVQGIRLWLLGHSWRQYLRQLALPGISSEASLMPIGVVLVLLYDPEQPLGFML"
BASE COUNT         1376 a         2549 c         2732 g         1429 t
ORIGIN      
        1 tgggccaacg acaaagacgt gtggaagctg tcggtcgaga cgctctcgac gaagaacgcg
       61 ctcgacattc aagtctccgc gatcgagggc gtcgcgctcg agctcgagat cgccgatggc
      121 gtcggcaaca cgctcgccac acgcaaggcc ccaaagggcg cgccgctgat cgtgaagagc
      181 ctcatgccgg tcattcccga aggcgcgccg ccgtttcact acgtgacggt caaaggctcg
      241 gcgtcgaatc ccgagacgtc gtacacgctg cacgcgacgg cgaggcccgt cggcaccgac
      301 gaggaagtcg agccgaacga tctcgtcgac aaaccagccg agatgaatcc cgagcggacg
      361 aaagtctacg cgacgtggac gccgggcgac gtcgactgct tcgcgctcca gcccgcgcag
      421 gtcgagcgca ccgtcgacat caccgtcgat acgcccgccg agatcgatct cgcggtcgag
      481 ctgctcgcag aaggcaaagc gatcggctcg gccaacgccg gcaagaaggg cgtcgtcgag
      541 aagctcagcg tgaaggtgcc gccgaacgtg aagccggtca ttcgggtgaa gaaccccgac
      601 gcgacggcga cggcagaagc gaagtacgag ctgtccgtca tcgaagcgac gacgggcgac
      661 aacgtgccgt gagctgggaa cccgacacgc tgcagcacta cttacagacg caacgcggct
      721 tcggtcgcgt gttctgcgat tggcgtgagc ggtcgcgcca gggtgagccc gagccgaccg
      781 agctcgacga gtatgtcgcg tcgctgttcg gcgacgttct cgacgaccaa tgggcccgcg
      841 gtggctaccg ccaggtcagc gctcgcgaag cgcgccacca actcgcattc tggctcgaga
      901 aaaacctcga ggagggggcg gcgccggctg gctcgatgct cgagtcggct gcgatcgagc
      961 cgttcttcaa gctgttcggc gcggggacga gctacttcac gaatgcgtcg atgcagttcc
     1021 cagcacacgg cacaagtagc gcattcatga cgaagacgct gcacgactac gacgccggcg
     1081 tgatcgccgt cgacgatgtt cgtattggcc tcgtctggtt tgccgacgac gaatagcgcc
     1141 gtgagctacg acatcatccg cttgttcgcg ctcgactgga cggcgaccgt cgaggctctc
     1201 cgcacgcaag gcgacgactt cttctggctc acgcgcaccg cgaacggaga cgacatcgac
     1261 ttcagtcgga ttccgttcgt ggccgagttc gagggtgccc ttgcgagcat gccggggtgg
     1321 cgcgacccga ccgcgggcga gatttacgac aaccttcgcc agcacctcgc tgcagatgct
     1381 cgcgagaggt gcgacgcgtg cttctcgcgc ctgttctggg agggcaactc gccgttgacg
     1441 cttcggggcc tccgcctccc cgagggggct ccaccggcgc atatctggaa cgcggtttca
     1501 ccgtcgtcgg tgcgcgacgt cctcggttgg atccaacgca tcccgtggcc ttcgctgcac
     1561 gcggtcgccg atcggtatac gggttgggag aaccggtacc tgccgaccat cgatgacttc
     1621 gaatcccagg tcggatgtca cgaagagctc ttgcgcgagg ccatgaagcg aaatgccggc
     1681 gtgctctcgc tcattagcgc atgatcgacg gggtagccat gcggccccgc atctgggatt
     1741 ctgcgcgtga gtcatgggta gcggtgacac aggacggcca catgccgccg gcgcgcaagc
     1801 cggactcgtc ggcgacgtta tagctgcctc cggcggcgag cccagcccac gcgagtcgct
     1861 gtggatgcgg cgcgccggcg gtccgcgcat cctcgttctg ctcgcgctga cgaacctcgt
     1921 cgcgtacgcc gtgcgcaacg cgctgttcgg cgtgtatccg gatctgcgcg ccgagtttca
     1981 cgtcaacgac cagacgctgg gtttcctggc gaccgtcttt ttgatcccgc atgcggtggc
     2041 gacactgccg ttcgggtggg cgggcgaccg gtatgaccgc cggcgcgtga tcgcctttgg
     2101 catcatgctg tccgccgtgg cgagtgtcgc gggcgctgca tcgcgcgata tgtggacact
     2161 cggcatttcg cgcgcggcag tcggtctcgg gaccgcggcg gttgtcccgg tcgcgaactc
     2221 gatcttgggg cagctgtatg aagggccgcg caaggcgagc cgcatcgcac tcttcaatct
     2281 cggcatcctc gtcggcgggc tcgtcggctt cggtgccggc atcttcgtcg ggtatccggc
     2341 cgtcgtggtt gtgctcgctg caccgatggt ggtgctcgcg ctgctggtgc tcgcgatgcc
     2401 gattccggcg cccgtggcgg cgcgtggccc aatgccgcgg gcacaagagc atgtcgggtt
     2461 gctgcagtat ttgtttcggc tcgcgaaggc gttcttcgtc gacggcaaga tgctgctgcg
     2521 gattcgtacg ctccgttggc tgatcgtgtc ggcgaccgcg atggcatttg cggcgggcgg
     2581 gttcaacgcg tggctcctcg attttctcga gcgcgacaag cagatgacgc gagcgtcggc
     2641 gactacgctc atctcggttg cgatgatcgg cgccgtcgcg gggatcgtcg tcggtggacg
     2701 cctcgcggat cggctgcgca cgagaatgat gacgggccgg ctctggacca tcgcgctcgg
     2761 catgttgttc gcgattccgt gcaccgcgat ctgcatcgag atcgatccgg gcgtcggcct
     2821 ctacgtcgcg ggcacgctca acttcttctt cttcagctgg tatcacgcgc cgatcgcggc
     2881 gaccgtcgat gatctcgcgc cgccggtgct cgcagtgtcc gcgcaaggcc tcgtgatctt
     2941 cacgatgcac ctgttcggga cggcgagcgc gagctacgtc gtcggcatcg tgagcgatca
     3001 ctcatcgctc tacacggcga tgtgggtccc agcgggagca ctcgtgctcg ccgggctgac
     3061 ggcgctgtgc gcgattccga gcttcgctgc cgaccacaaa gcgtcgcgcg ctaatccagc
     3121 agcgtcgtga tctcggggcc gtcggggccg atcaagatcg tgtgctcgaa ctgcgcggag
     3181 agcgatccgt ccacggtgcg cacggtccag ccgtcggcat cgacgcgagt cgcggacgtg
     3241 cccgcattga tcattggctc gatcgtgaac gccatgccct ctcgcaagcg atggccgaag
     3301 tgcgggcgtc ccacgtgcgc gacgtgcgga tccatgtgca tcgcacggcc gataccgtga
     3361 ccaccgtacg tctcgacgac gctgccggca gtgccgacga agcgcgaaat tgcggtcccg
     3421 atatcgccga ggcgtgcgcc gagaaccgct gcggcgatgc cagcgtcgcg cgcgccacga
     3481 gcgagctcga cgagagcacg tgcatgcggc ggtgcatcgc cgacgatgaa cgtcgccgac
     3541 gtatcgccgt gatagccgtc gagctccgtc gtgacgtcga tattcacgat gtcgccgtcg
     3601 acgagaagga cgtcgtcgct cgggacgccg tggcagacga tgtcgttgcg gctgacgcaa
     3661 acggcggcgg gaaagccgtg atagccgagc tgcgagggcc gcccgccgag ggcgtcggtg
     3721 tgcgcacgca cgaggcgatc gatttcggcc gttgtcatcc ctggcacgag ccgcgcgccg
     3781 acgtacgcga gcgtggccgc cgctgcccga cctgcacgtc gcatgcgatc gcgttcgtcg
     3841 cgcgagagca gcgtgatggc catgcgtgaa cgtgcgatgg aatcgcgtcg ccgtccaata
     3901 ccttttcgga ctcgatgcgg cgacgaacgt ggggcatgat catcggcgat gagcttgcag
     3961 cagttgcagt acttcgtcgc cgtggcagag gagcagcacg tcacgcgcgc cgcagagcgg
     4021 ctccacatct cgcagccacc gttgagccgg cagatccagg cgctcgagga cgagctcggt
     4081 cagccactct tcgaacgccg cggccgcggc atcgtgctca cggagttcgg ccgctacttc
     4141 gcggcgaagg ccgccgccat cctcgaccac gtcgacagcg tgctggccga caccaaacgc
     4201 tggtcgagct agcgcaagcc gttcacgagc accgcttcgt actgggcagc gcgcgcgaga
     4261 agctccgcgc ggtgtgcgtt ggcggcgatc gcgcggggca gggcggtgac gtcggcctgc
     4321 tcgccgaagt acagcgatgc gagcgtgagc gcgttgctgc gatcgttggt tggcagcgcg
     4381 gcgatctgcg ctccgtgatt cgcgccgggc acgaagtaac gcctactcga cggtttcgtc
     4441 ggtgggtcca tcgcgccgcc actccacgga tcgagctcgc cgtagatcag caacaggttg
     4501 tccgcggact tcgcccacgt gcgcacgtcg cgcgtgacct ggccgttgta cgcgggcaac
     4561 gcgatgtccg gatactggga cttgaatgcg tcttccatgt tcatacgcgc cgatggatcc
     4621 gtcacgtagg acgccaccgt tgggctgagc tgctgcgcga agccttgctc ggtgagccac
     4681 tcgtacgaga gcgcgccgtc ggaccgagca tcggttgccg ggagacgctc gggatagcca
     4741 ctcgcctggc ggaggaacgc gaagaagttg tcgtctgtcg tcgtcgacgt cggaaccgcc
     4801 gcgcacgatg tgttgccgta gtactgccag aagctccagt cgaggtagcc ggccatcgac
     4861 tgcagatatg actcttcggc gcctgcgccg gcgctggccg tgagcctcgt gatcatcgcg
     4921 ttgtgtcggg tcgtaagcgc tgcaacctgc gtgtcgcgga tcttctgcgc gcagctcgag
     4981 gtcatgtgct gcgtgatgta cgactggtac tcgttgtcga cccgagcgcg cgacgcgggc
     5041 gcgacgtacg gaatgcttcc gtcgagatca tgcggaaaga agtagtagtg gtaggcggcg
     5101 gtgatgccgc ctttgctcgc gcccgtcgag acccagtttg cgccgtagat ggtcttgaac
     5161 tgctcgatca cgcggtgcgt gtcgttcgcg ccgttttcga tcgtcagtgc gctccagtcc
     5221 cacgcggctt gcgccggaac agactcgcct tgatagcgat gctcgagcca cagcgtgttc
     5281 gcgtggaaga gcgtcgcgag ctcgtcatcg aagaagccga agctcgagta gccccaatcc
     5341 gcgacgacca tcgggcgatt gcagccgcga tgcgtgagca gcaaatgctg gtcgtacgtg
     5401 gcagggttcg tcgcgctaat cggctggcgg aacgtcacgt cgaagcagcg agccggcgcg
     5461 gcgacatact ggccgcacgc aacctctctc gagctcgtca cgttcgcgag agcgctgagc
     5521 tcggcgggca gctctgcttc gtcgcagacg gcggcgaccg gaggctccgg ttgatcgggg
     5581 ccgtcaggcc catcgtcttc gatctgcgcg ccgcaacctg cggcaaggac gagcgtcgcg
     5641 agcaaccgct gcatgggctc gatgctgtaa agcaagaccg agacccggcg ccttcgcggg
     5701 cgatgcactt ggtgcgcatc ggcgcgtgtc gaagcgtggc gcgagtccga tgtgtatggg
     5761 ataacgcggg cgtgcgttac gcactgtgcc tctgcctgct ggtcgttgcg gcttcgtgcc
     5821 ggcctgcgaa gggtccgtcg cagacgctcg acaagtacgg ccgcgcgctg aagaaccatg
     5881 acttcagctc ggcgtacgac ttcatgtcgt cgagctttcg cggaaaggtc tctcgcgagg
     5941 actacgtccg catgatgcgc gacaacccgc gcgaggtcga tgagaccgcc gaccgcttgc
     6001 gcggcaagca cggcagcctc gaagtgtctg ccgagttcga gtacgggctc ggcgatcaga
     6061 tgcgtctcgt ccaagagggc gggcgctgga agatcgcgag caacccggtc gcgttctacg
     6121 accagtcgtc gccgaagggc gcgctgcgct cgttcattcg ggcgtaccgc ctcgagcgct
     6181 gggacatcat gctgcgcttc gtgccgaatc aatatcgcga gaagatggac ggcgcgaagc
     6241 tgaagacgca gttcctcggc ccgagcaagg agcaggtcga gacgctcatg aacacgctcg
     6301 aagcgaacgt cgacgagccg atcacggagc gcggaaacaa cgcacgcatg agctacggcg
     6361 accgtttcga ggtgaagttc ctcaaagagg acggcgtctg gaagctccaa gatctcgact
     6421 gagactttca ggctcaatca ctggcggatc gaatagccac catgtgggca aacccccacc
     6481 tggtggatct gtcaggcgcc agattcgtca cttgtgctcc gcaaaaaggt aagggtgtgg
     6541 agaacaggca caacgtggcg cgcgcgttca gataacgtcg acggcgacgt gcgtcagtga
     6601 ggcttgtaga cccgcgcatc gccacacttg cgacgatctc gtggcctcca caaatcgtat
     6661 gtgtgaaatg gagcccggcc gaattccatg cgttagaatg aaggagtcgg gtgcgacgtc
     6721 caaggcccgt aggttgcagt ggtggaggtt aggagctacg cagagataga gcattagata
     6781 gttaggctcg accgcagtcc cccacgaaag gtggtcccgt catgctggat ccctggatta
     6841 tcgaagagat cctcaagaaa gaagaggaac ggcgccgtga gagtgaacgg agtcgagtcg
     6901 agctgcctat cgagcgcgaa cgcgataccg gtccacggac cgtggtgacg ccaccccgcg
     6961 aggagagcga acgcggtgtg atgatcatcg acatctgatt catcaaacct caaccgaaag
     7021 ccctcacacc caacggtggc gagggctttc gcgcttttcg ggcccgcaaa gatgataagt
     7081 acgcgcggtg gcgtcgcgta acggcaatac gggcggcgat cagcgccggg ccgacagcac
     7141 gccagcggcg acctcgcgcg cgaagcgagc aagtaatcca ccgccggcgc aaggcgctcg
     7201 aggctccgcc gccgatgcgg gaaagcgcga gctcgagagc ggtgagcgtc cgcgtgcgaa
     7261 gcgcgacacc ggtggaccga gcgagtcatc acgcatggcg cggttcgcaa cgggcgaatc
     7321 gtcgagcggt ctgcggcgcg ctggaacggc gaacccgcgc cctcgttcga atacgagtgg
     7381 tggtggccgc gcaggatccg agtcggcgat cgtcgttccg aatcggcgct ggatcggcca
     7441 cacgttcacg atcgcgctcg gcatcatcgg ctggctgatc gtcctcgtcc tcccgctgac
     7501 gggcctcgtc ccagtcgacg aagcgacgcc ggcgctgccg atcatgatgt tcgtgctcgt
     7561 catcctcgtg gcgcgcgcac tggcattccg gctgacagaa ggcagcgtgc tgtcgctcga
     7621 ttccgcgtat tacgtagcgg cggcgttatg cgtcgggacg gctgaagccg gacgcctcgt
     7681 tgcattggcg ctcacgctcg acgccagcgc acgcctctcg agcaaacgcc gtcgcaaggt
     7741 cgacctcgat ggctggtggg ccgagctcgg ctacgtcctc tacttcggcg gcatgagcgg
     7801 tggcttgctc gtggtgtgcg gctgggtgtt tggcgcagac agcgtcgtcg gccagatgcc
     7861 acacgagccc gtcgagatca cgatgcgcgt tgtcgcgatc gccggcatgt tgctcgtcac
     7921 ccactacgcg gtgcagggca tccgcctctg gctcctcggt cacagctggc ggcaatatct
     7981 gcggcagctc gcgttgccgg gcatctcgtc cgaggcgtcc ctgatgccga tcggcgtcgt
     8041 gctcgttctg ctctacgacc ccgagcaacc gctcggcttc atgctg
//
LOCUS       DATLBZ010000007        12138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4477586, whole genome shotgun sequence.
ACCESSION   DATLBZ010000007 DATLBZ010000000
VERSION     DATLBZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12138
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4477586"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VMZ53_00225"
     CDS             <1..1086
                     /locus_tag="VMZ53_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HUS26891.1"
                     /translation="QAGMRSRVYHAGLEDEVRVKAQDVFMAGQLDVIVATNAFGMGVD
                     KSDIRLVVHADIPRSPEAYYQEAGRGGRDGKPTRCVLLFNHGDIRLQEFLIDASFPST
                     ELLRGMWKLLQTQPALGKGTDDEVEARLKSKLPESPSGATISSAIRILERHGMLVRNA
                     DDSGIAAARPQPGLYPPLEVESLKRRAESERGKLRTMVEYAYYPRCRRQYVLEYFGDA
                     DWMARDRRCGACDNCEAVANGKATGLSESELESIRNLLLLVGSLHGRFGRTRIAALAN
                     GSDDDARFEEMPHRGCLRGWSTQHIMDLLRALEGAGLVEASRGEYPTLSTTRRGDQVG
                     IGKLDPNDLGLQMPTVTKRSRVRKRKR"
     gene            complement(1091..3208)
                     /locus_tag="VMZ53_00230"
     CDS             complement(1091..3208)
                     /locus_tag="VMZ53_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26892.1"
                     /translation="MRLSVVLLSVCVAGTAWAKGAAKPLVDPNKVADESAVFFEVGRS
                     GDGYWVHAIADVMGLEKDDTLRLDIRHGNQTGTQKCTLSTSNAADADTVECTGSSGGK
                     IFTGAGPIEADLILHSDKDDKEYVVRTFKGNVKNWDGKGEFQIDGDDMLGAAYYVPHD
                     VQGTGRPTLRFWITGDSFGTSSLRCKVDGKPLPGDINGDVESRYGFNADIIKGNSRTI
                     WKWTQVTFSAARWVVKKVPNAQDEYWSSEMYPGTWECQLRHNGVGVREFKFVVDNKGE
                     VAPNAMQTAKGSPKLPAHYAMIDMKIVPGNGIDQRIKPDAIKKSRGYGMPWPESDATK
                     ALVAALPKAYENATPVAVKPKRQIIDGAKANPPRFVDESSTYIATIRSGDGYSVYGRA
                     GVIGVEGRSSEEYRIAWRQKGKEVGSTKCEQAASWMGVNMNDERKTIGMFGTEKATAI
                     TCSDSAMIYKATGPIEAQLIQYDDMDGNEYILRTYNVNVVHPKAYGDPLWMIFPDDTL
                     GAGWIAGNHKDAGSIIFRFWSTSSKAPDKARCTVDGKKVPDFKISGGGGDVSIEYDYR
                     KTQQSDQVKYGWYFMNLDPANGVSPGMPDKSDKTSADYLTALGEYPGNWVCDLRTDDA
                     KVLRTFSFHVNDKGYIDPSPSRAGELMSPDVVPVDMKFGKDALDTRVRPDAMKKSAGF
                     GRPWPKGPPKELPAKASGLPDLK"
     gene            3544..5652
                     /locus_tag="VMZ53_00235"
     CDS             3544..5652
                     /locus_tag="VMZ53_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HUS26893.1"
                     /translation="MASTTHVPGQAHGNNAADQAPGASANDLAAAVGLDCDCAPRVCI
                     GSCTGAAAGTACDDNDRCTGDGTCAGDTCEPGAATCVAGTAVDACTVQSGTCDGATGE
                     CFTDPLAAGTTCGDNDVCNGAGDCVECISDAQCAAGFVCSGAVCVGVPHVTINEIESS
                     GGVPGDWIELTNTGTAAADVGGWRVLDNDNTHVAYVIPAGTIVPIGGYVVLNEAQFVF
                     GLGAADSARLFDTTNTLVDSYSWTAHAVTTYGRCPDGTGAFKTTTSVTKGAANDCSIA
                     VHINEIESSGGTPGDWVELFNAGPISVDMSGWIFRDNDNTHAYAIPAGTTLAPGAYYL
                     LEEAAFGFGLGAADSARLFESNGNVIDTYTWTAHAATTYGRCPNGSGSFATTASSTKG
                     AANTCGGGGPTGSPWPGRNAVAAIDATGVSGVNVSGLFYDGTTLWAVRNNPSELLTYT
                     AGGALTSTRTLVYPNGSGVPDAEDLTKAELSSPAMYVVTERDNANDTVSRMSILRYDT
                     SASGTTLVATNEWNLTADIPTAGPNVGLEAITWIPDTFLVSHSFFDSSKSHAYNPAEY
                     ANHGTGLFFVGVEATGQIYVYALDHATSTFTRIASFSSGDPVNKALYFDRDVGYLWTY
                     CGSSCGNQAGVMTLGASGVFSLIKQIARPSSMANLTNEGIAIAPESQCSGGQKTFWWT
                     DDGAADGHALRADTIPCGAF"
     gene            complement(5649..6350)
                     /locus_tag="VMZ53_00240"
     CDS             complement(5649..6350)
                     /locus_tag="VMZ53_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26894.1"
                     /translation="MADSDDVLKKAGGLLNKLGKAAKEAGEKVAAGVKENAPKAVGAM
                     NKAGNAIADGVKENAPKAVEALNKAGDVIVDKTKQVTGLGRGQIKLELDQTRAAPGGT
                     ITGRLVLDLKEPVEAKRLVVALTAHQRMVNVTKDSGHRSVGTTSANVYNFEVELGGAT
                     TYKSETLSFELTIPPDALDLKASPPTTPLGDVVRSVAAAVSPTAGPIQWQVAGRMEIP
                     WGRNLTHDVDIVVTR"
     gene            6381..8672
                     /locus_tag="VMZ53_00245"
     CDS             6381..8672
                     /locus_tag="VMZ53_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HUS26895.1"
                     /translation="MSADDDLDVAAPPRAAGGLGALKATLTHLQRDSGLARGIKGLLS
                     MNQEGGFDCPGCAWPEPKTGGRARFEFCENGAKALAEEATTAKADPELFSRATVDELR
                     ELSDFELGRLGRITHPLILGEDRKYHAISWEHAIALTAAELQKAGPKHSAFYTSGRTS
                     NEAAFLYQLLGRMFGTNNFPDCSNMCHESSGNALLEVVGVDKGTVTLADFDHSDLIFV
                     IGQNPGTNHPRMLSTLREAAKRGCTIISINPLKEVGLSRFSHPQHPLDLLAGGVALAS
                     EFVQVQIGGDQAFFLGIGKAVLELDAANATESETAVKTVKTDATVSTDMLFLADKTDN
                     FFTWREHAMATPWSAIVEGSGVDEATIRRIAEHYAKAKSVIACWAMGITQHKHAVRTI
                     HEIVNVMLLRGNIGRAGAGLCPVRGHSNVQGDRTMGIDHHTRHPFLDRLGAAFDFEPP
                     RVAGYDTVGTIAAMERGEVRAFVMLGGNWVSATPDTHRTARLLERCELTVSISTKVNR
                     THLYPGKHALILPCLARSEVDGGQFVTVEDSMSMVHRSAGNLRPASPHLRSEPAIVAA
                     LGEALLGNRVPWRDYAENYDRIRDKISQVVLGCSDYNARVREPDGFQLPNTARDGSFN
                     NVGGRARFTVSPLPNLALPPGRLRMMTMRSHDQYNTTIYGLDDRYRGVRGERRVVFVN
                     PTDMIELGLHERQLVDLISEWHDGERIVEAFIVVPYDLPRRNCATYFPEANPLVPLDS
                     YADRSNTPTSKSVTIRIVPRLEV"
     gene            complement(8661..9800)
                     /locus_tag="VMZ53_00250"
     CDS             complement(8661..9800)
                     /locus_tag="VMZ53_00250"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS26896.1"
                     /translation="MDPSGTPTAQDTQVAKDLTDGLRSRAKAGTSQYQLATASDKELI
                     DQKLLNNCDTEAFACMSGIGNQLGADVLMYGNLKKDKNQYVVTLKVLDVATKKMLKTT
                     TDPIPVSESTGAALQGWAKKLYAKLTGEATGGTIVIRVANADRGTILINGEEKGSISS
                     GTGTVSGLPEGKVRVAVESDGFHRWEKDVTVTSGTQNIPVEMEKGVTGEVVGPGPGPG
                     PGPGPGPSDQTSRGSAMWKGVFIGTVLVGGGAIALGLKGNQMRDEAARAECAGGAYAG
                     HSIDGMACPEKKDVPMSMQLDAAGFKKWEDHGSKGQLYGYTGLSVAIAAGGFAVLAFY
                     KGFIEKNPSSSREHASRGHRVRRDRFTVTPVIAPSGAGATFRLDF"
     gene            9904..11484
                     /locus_tag="VMZ53_00255"
     CDS             9904..11484
                     /locus_tag="VMZ53_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS26897.1"
                     /translation="MRLLRSGSIHRPGPENLACQNWPGYRLEARMRTLSARILLGFAA
                     LTITFGAITGNIVYNQHTLESYVELVNTGLVPVSQQIRELARRCDDLRQYVSEGFDKE
                     SNVDSARYRLENLRLRRDKGFADVQKFFATYDDMDKLDSKHQLRKAVTDLDVPIRSTK
                     PLYDKLALAFQHGEPLESNAVNEGVGPLRDAERQISNQANSLATTIERSVAAGLRDLQ
                     ERETQSRHITIFLGAVAVLLGLLMTTWVVIALRPLRRLQNAARRVAAGDYGNRIEVRG
                     PAEVADLARELNTMATAVEERERELVRSERLAAVGKMAAMITHEVRNPLSSIGLNTEL
                     LEDEVEGNEEARDLVRAIHREVDRLTAITEEYLTFARLPKPKLAPEAINPLVGALAAF
                     VRGDLAVKKVDLKVDLAPGDPIAIIDAGQVRQCLINLVRNASEALGAKGSGSVTLRTR
                     SEGDRIVIEVEDDGVGIPSDVLPRLFDPFFSTKEGGSGLGLALTQQIVKDHGGDLGVQ
                     STLGKGTTFTVSVPAKAG"
     gene            11507..>12138
                     /locus_tag="VMZ53_00260"
     CDS             11507..>12138
                     /locus_tag="VMZ53_00260"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS26898.1"
                     /translation="MSDNDDKRRSDREAVTLFVEYEGADDLLGDYTENLSSGGTFVAT
                     NRELPIGTQIQLVLSFPGLLEPISIVGIVRWTRGNRGAGDVVEGGEEAGAGIEFAPGP
                     ARDQLAAMIEKIRERDPKTVSRVYKVLVVEDNRHVAQLIQDGLRGSARREFGGGVSFD
                     VRNAEDGRQAIEILRKDTFDALIVDVYLPVVDGAKVISTARTDLALTNLP"
BASE COUNT         2068 a         4038 c         3889 g         2143 t
ORIGIN      
        1 caagcgggca tgcgctcgcg cgtctatcac gcaggtctcg aggacgaagt gcgcgtgaag
       61 gcgcaagacg tgttcatggc cggccagctg gatgtgatcg tcgcgacgaa cgcgttcggc
      121 atgggcgtcg acaagagcga catccgcctc gtcgtccacg cagacatccc gcgcagcccg
      181 gaggcgtact accaagaagc gggtcgcggc gggcgcgatg gcaagcccac gcgctgcgtc
      241 ctgctcttca atcatggcga catccgcctg caagagttcc tcatcgacgc gtcgttcccg
      301 tcgaccgagc tgctgcgcgg catgtggaag ctgttgcaga cgcaacccgc gctcggcaaa
      361 ggcaccgacg atgaagtcga agcgcggctg aagtccaagc ttcccgagtc accgtccggc
      421 gcgacgatct cgtcggcgat tcgcatcctc gagcgtcatg gcatgctcgt gcgcaacgcg
      481 gacgacagcg ggatcgcggc tgcccgcccg cagcccggtc tctatccgcc gctcgaggtc
      541 gagtcgctga agcgtcgcgc cgagagcgag cgcggcaagc tgcggacgat ggtcgagtac
      601 gcgtactacc cgcgctgccg ccgtcagtac gtgctcgaat acttcggcga cgccgactgg
      661 atggcgcgcg atcggcgctg cggcgcctgc gacaactgcg aagcagtggc caacggcaag
      721 gcgacgggcc tgtccgaatc ggagctcgaa tcgatccgca atctgctgtt gctcgtcggc
      781 tcgctgcacg gccggttcgg ccgcacccgg atcgcggcgc tcgcgaacgg cagcgacgac
      841 gatgcgcgct tcgaagagat gccgcaccga ggttgcctgc gcggttggtc cacgcagcac
      901 atcatggatc tgcttcgcgc gctcgaaggc gccgggctcg tcgaggcatc gcgaggcgaa
      961 tacccgacgc tgtcgacgac gcgccgtggc gatcaagtcg gcatcggcaa gctcgatcca
     1021 aacgatctgg gcctgcagat gccgacggtc acgaagcgct cgcgcgtgcg taagcgcaag
     1081 cgctgagctg ctacttcaga tcgggcagac cgctcgcctt cgccggtagc tctttcggcg
     1141 ggcctttcgg ccacggacga ccaaagcctg cgctcttctt catcgcgtcc ggccgcacgc
     1201 gcgtgtcgag cgcatccttg ccgaacttca tatcgacggg cacgacatcg ggcgacatga
     1261 gctcgccggc gcgtgacggc gacggatcga tgtagccctt gtcgttcacg tgaaacgaga
     1321 acgtgcgcag gacctttgcg tcgtccgtgc gaaggtcgca gacccagttg cccggatact
     1381 cgccgagggc cgtgaggtaa tccgcgctgg tcttgtcgct cttgtccggc atgcccggcg
     1441 acacaccatt cgcagggtcg aggttcatga agtaccagcc gtacttcacc tggtcgctct
     1501 gctgcgtctt gcggtagtcg tactcgatcg atacgtcgcc accgccgccg ctgatcttga
     1561 agtccggaac cttcttgccg tcgaccgtgc aacgcgcctt atccggagcc ttggaggagg
     1621 tgctccagaa tcggaagatg atcgatccgg catccttgtg gttacccgca atccaccctg
     1681 cgccgagcgt gtcgtccggg aagatcatcc acagcggatc gccgtatgcc ttcgggtgca
     1741 cgacgttcac gttgtacgtg cgaaggatgt actcgttgcc gtccatgtcg tcgtactgga
     1801 tcagctgcgc ttcgatcggt ccggtcgctt tgtagatcat cgcggagtca ctacatgtga
     1861 tggcggtcgc cttctccgtg ccgaacatgc cgatcgtctt gcgctcatcg ttcatgttga
     1921 cgcccatcca cgacgccgcc tgctcgcact tcgtcgagcc gacctccttc cccttctgcc
     1981 gccacgcgat cctgtactcc tccgagctgc ggccttcgac gccaatcacg ccggcgcggc
     2041 catacacgct gtagccatcg ccgctcctga tcgtcgcgat gtacgtcgac gactcgtcga
     2101 caaaacgcgg cgggttcgcc ttcgcgccgt cgatgatctg gcgcttcggc ttcactgcca
     2161 ccggcgtcgc attctcgtat gccttcggca gcgccgcgac gagcgccttc gtggcgtcgc
     2221 tctcgggcca cggcattccg tagccgcggc tcttcttgat cgcgtcgggc ttgatgcgct
     2281 gatcgatgcc gttgcccggc acgatcttca tgtcgatcat tgcgtagtgc gcgggcagct
     2341 tcggcgagcc cttcgcggtt tgcatcgcgt tcggcgcgac ctcgcccttg ttgtcgacga
     2401 cgaacttgaa ctcgcgcacg ccgacgccgt tgtgacgcag ctggcactcc cacgtgccgg
     2461 ggtacatctc cgacgaccag tactcgtcct gcgcgttcgg caccttcttc acgacccagc
     2521 gagcagccga gaacgtcacc tgcgtccact tccagatcgt gcggctgttg cccttgatga
     2581 tgtcggcgtt gaagccgtag cggctctcga catcgccgtt gatgtcaccg gggagcggct
     2641 tgccgtcgac cttgcagcgc aatgacgagg tgccaaacga gtcaccggtg atccagaagc
     2701 gcagcgtcgg acggccagtg ccctgaacat cgtgcggcac gtagtacgcg gcgccgagca
     2761 tgtcatcacc gtcgatctgg aactcgccct tgccgtccca gttcttcacg ttccctttga
     2821 acgtgcggac gacatactcc ttgtcgtcct tgtcggagtg caggatgagg tccgcctcga
     2881 tcgggcccgc gccggtgaag atcttgccgc cgctgctgcc agtgcactcg acggtgtcgg
     2941 catccgcagc gttgctggtg ctcaatgtgc acttctgcgt gccggtctga ttgccgtggc
     3001 gaatatcgag acgaagcgtg tcgtccttct cgagccccat cacatcggcg attgcgtgga
     3061 cccagtagcc gtcaccgctg cggcccacct cgaagaacac cgccgactcg tcggcgacct
     3121 tgttcggatc gaccagcggc ttcgccgcgc ccttcgccca cgcagttccg gcaacacaaa
     3181 cagataacag caccacactg agacgcatga atcctcccgc gcgggacgct actcgctgcg
     3241 cagcgaggtg cgcaagatcc tccgcgagat cgatctcgca cagcaccaat tgacaaccat
     3301 cgacggaacc ccgaaacact tccgctcatg ttcaatcgcg tggtggcgat gtgtcttgtc
     3361 gtttcggctg cgtgtggaac ggagcgagaa gcttcgacga cgcagcaagt cgcggcaggc
     3421 ttgtacgatt gcgcgagccc gacacagcgg ctcacctgcg atcctccggc cgatccggcg
     3481 ttgcgtttcg tttgccatgc gacgcactcg gcaaagaacc cattcgtcaa gatcagcgtt
     3541 gacgtggcgt cgacgacgca cgtccctggt caggcacacg gcaacaacgc cgccgaccaa
     3601 gcgcccggcg cgagcgcgaa cgatctcgct gccgcggtcg gcctcgattg cgactgtgcg
     3661 ccgcgcgtgt gcatcggcag ctgcacggga gcggcagccg gcaccgcatg cgacgacaac
     3721 gaccgctgca cgggcgacgg aacgtgtgcg ggcgatacgt gtgagccagg cgctgcgacc
     3781 tgcgtcgcgg gtacggccgt ggatgcgtgc accgtgcaga gcggaacgtg cgacggcgca
     3841 accggcgagt gcttcacaga tccactcgcg gccggcacga cgtgtggcga caacgacgtc
     3901 tgtaatggcg cgggcgactg cgtcgagtgc atcagtgatg cgcagtgcgc ggcggggttc
     3961 gtatgcagcg gcgcggtttg cgtcggtgtc ccgcacgtga cgatcaacga gatcgagtcc
     4021 agtggcggcg ttcccggaga ctggatcgag ctgacgaaca cgggcacggc cgcggcggac
     4081 gtcggcggtt ggcgcgtgct cgacaacgac aacacgcatg tcgcgtatgt gatccccgcc
     4141 ggcacgatcg ttccgatcgg cggctacgtg gtcctcaacg aagcacagtt cgtgttcggc
     4201 ctcggcgccg cagattccgc gcggctgttc gatacgacga atacgctcgt cgactcgtat
     4261 agctggacgg cgcacgccgt gacgacgtac ggccgttgtc ctgacgggac cggcgctttc
     4321 aagacgacga cgagcgtgac gaagggtgcg gcgaacgact gcagcatcgc ggtgcacatc
     4381 aacgagatcg agtcgagcgg cggcacgccc ggagactggg tcgagctctt caacgcgggt
     4441 cccatcagcg tcgacatgtc gggctggatc ttccgcgaca acgacaacac gcacgcgtat
     4501 gcgattcccg cgggaacgac gcttgcgccc ggcgcttact acctgctcga agaagcagca
     4561 tttggcttcg gcctcggcgc tgccgactcg gcgcgactgt tcgagtccaa cggcaacgtg
     4621 atcgacacgt acacgtggac cgcgcatgca gcgacaacgt acggccgctg cccgaatggc
     4681 tcgggctcgt tcgcgacgac agcaagctcg acgaagggcg cggccaacac gtgcggcggc
     4741 ggcggtccga caggctcgcc gtggccaggt cgcaacgccg tcgcggcgat cgacgctacc
     4801 ggggtgagcg gcgtcaacgt cagcggactc ttctatgacg gcacgacact gtgggcagtg
     4861 cgcaataatc ccagcgagct gctgacgtac acggccggcg gagcgctgac ctcgacgcgc
     4921 acgctcgtgt atccgaatgg cagtggtgtt ccggacgccg aggatctcac gaaggccgag
     4981 ctctcctcgc cggccatgta tgtcgtgacg gaacgcgaca acgcgaacga caccgtgagc
     5041 cgcatgtcga tcctgcgtta cgacacgagc gcctcgggca cgacgctcgt cgcgacgaac
     5101 gagtggaatc tgacggcgga catcccgacg gccggtccaa acgtcgggct cgaggcgatc
     5161 acctggatcc ccgacacgtt cctcgtttcg catagcttct tcgacagctc gaagagccac
     5221 gcctacaacc cggcggagta cgccaaccac ggcaccggct tgttcttcgt cggcgtcgag
     5281 gcgaccggac agatctatgt ctacgcgctc gatcacgcga cgagcacgtt cacgcgcatc
     5341 gcgtcgttct cgagcggcga tcccgtcaac aaggcgctct acttcgatcg cgacgtcggc
     5401 tatctctgga cctactgtgg ttcgagctgt ggcaaccagg ccggcgtgat gacgctcggc
     5461 gcaagcggcg tgttctcgct catcaaacag atcgcgcggc cgtcgtcgat ggcgaacctc
     5521 acgaacgaag gcatcgcgat cgcgccggag tcgcagtgca gcggcggcca gaagaccttc
     5581 tggtggaccg acgacggcgc ggcggacggt cacgcgttgc gcgcggacac gattccctgc
     5641 ggcgcgttct agcgcgtcac gacgatatcg acgtcatgcg tgaggttgcg gccccacggg
     5701 atctccatgc gacctgcgac ctgccactgg atcgggccgg cggtcggcga gaccgctgca
     5761 gcgaccgacc ggaccacgtc gccgagcggc gtcgtcggcg gtgacgcctt gagatcgagt
     5821 gcatcgggcg gaatcgtcag ctcgaacgat agcgtctcgg acttgtatgt cgttgcgccg
     5881 ccgagctcga cctcgaagtt gtagacgttc gcgctcgtcg tgccgacgct gcgatggccg
     5941 ctgtccttcg tgacgttgac catgcgctgg tgagcggtga gcgcgacgac gaggcgcttg
     6001 gcttcgacgg gctctttcag atcgaggacg aggcgaccgg tgatcgtacc gcccggtgcc
     6061 gcacgcgtct gatcgagctc gagcttgatc tggccgcggc cgaggccggt gacttgcttc
     6121 gtcttgtcga cgatcacgtc gccggccttg ttgagtgctt cgaccgcctt gggagcgttc
     6181 tctttgacgc cgtcagcgat cgcgttgccc gctttgttca tcgcgccaac tgctttcggc
     6241 gcgttctctt tcacaccggc tgcgactttc tcgcccgctt ctttcgccgc cttgccgagc
     6301 ttgttgagga gcccgcccgc tttcttgagg acgtcgtcgg agtccgccat ggctggagtt
     6361 tgccatgcta agcatggcgc gtgtccgccg acgacgacct cgatgtagct gccccgccac
     6421 gtgcggccgg cggcctcggg gcgctgaagg cgacgctcac gcatttgcag cgcgattcgg
     6481 gcctcgcacg cggcatcaag ggcctgctct cgatgaacca ggagggcggc ttcgattgcc
     6541 cggggtgcgc gtggcccgag ccgaagaccg gcggccgtgc gcggttcgag ttctgcgaga
     6601 acggtgcgaa ggcgctggcc gaggaagcaa cgacggcgaa ggcggatccc gagctgttct
     6661 cgcgtgcaac cgtcgacgaa ttgcgcgagc tgtcggactt cgagctcggg cgcctcggcc
     6721 ggatcacgca tccgctgatc ctcggcgagg atcgcaagta ccacgcgatc agctgggagc
     6781 atgcgatcgc gctcacggcc gcggagctgc agaaggcggg ccccaagcac agcgcgttct
     6841 acacgtcggg gcgcacgtcg aacgaagcag cgtttctcta tcagctgctc gggcgcatgt
     6901 tcggaaccaa caactttccg gactgctcga acatgtgtca cgagtcgagc ggcaacgcgc
     6961 tgctcgaggt cgtcggcgtc gacaagggca ccgtcacgct cgccgatttc gatcacagcg
     7021 acctgatctt tgtgatcggc cagaatccgg gcacgaatca tccgcgcatg ctgtcgacgc
     7081 tgcgcgaagc agcgaagcgc gggtgcacga tcatcagcat caatccgctc aaggaagtcg
     7141 ggctgtcgcg gttctcgcat ccgcagcatc cgctcgatct acttgccggt ggcgtcgcgc
     7201 tcgcgagcga gttcgtgcag gtgcagatcg gcggcgatca ggcgttcttc ctcggcatcg
     7261 gcaaggcggt gctcgagctc gacgccgcga atgcgaccga gagcgagacg gccgtgaaga
     7321 cggtcaagac cgacgcgacg gtctcgacgg acatgctgtt tctcgccgac aagaccgaca
     7381 acttcttcac ctggcgcgag cacgcgatgg cgacgccgtg gtcggcgatc gtagagggca
     7441 gcggcgtcga cgaagccacg atccgtcgca tcgccgagca ctacgcaaag gcgaagtcag
     7501 tcatcgcgtg ctgggcgatg ggcattacgc agcacaagca tgcggtgcgc acgatccacg
     7561 agatcgtcaa cgtgatgctg ctgcgcggca acatcggccg cgccggcgca ggcctctgcc
     7621 ccgtgcgtgg ccactcgaac gtgcaaggcg atcgcacgat gggcatcgac catcacacgc
     7681 gccatccgtt tctcgatcgg ctcggcgccg cgttcgactt cgagccaccg cgcgtcgccg
     7741 gctacgacac cgtcggcacg atcgcagcga tggagcgtgg tgaggtccgc gcgttcgtca
     7801 tgctcggcgg caattgggtg tcggcgacgc ccgatacgca ccgcacggcg cgcttgctcg
     7861 agcgttgcga gctgacggtc agcatctcga cgaaggtcaa ccgcacgcac ctgtatccgg
     7921 gcaagcacgc gctgatcctg ccgtgcctcg cgcgcagcga agtggatggc ggccagttcg
     7981 tcaccgtcga ggattcgatg tcgatggtgc atcgctcggc gggcaacctg cgtcctgcca
     8041 gtccgcatct gcgcagcgag ccggcgatcg ttgcggcact cggcgaagcg ctgctcggca
     8101 atcgcgtgcc gtggcgtgac tatgccgaga actacgaccg catccgcgac aaaatctcgc
     8161 aggtcgtgct cggctgctcg gactacaacg cgcgcgttcg cgagcccgat ggctttcagc
     8221 tgccgaacac ggcgcgcgac ggctcgttca acaacgtcgg tgggcgcgcg cgcttcacgg
     8281 tgtcacccct gccgaacctc gcgctgccgc caggccgcct gcgcatgatg acgatgcgca
     8341 gccacgatca atacaacact acgatctacg ggctcgacga tcgctatcgc ggcgtccgcg
     8401 gcgagcggcg cgtcgtgttc gtcaatccca ccgacatgat cgagctcggc ctgcacgagc
     8461 ggcagctcgt cgacctcatc tccgagtggc acgacggcga gcgcatcgtc gaggcattca
     8521 tcgtcgtgcc gtacgacctg ccgcgccgca attgcgcgac gtacttcccc gaggcgaacc
     8581 cgctcgtgcc gctcgatagc tacgcggatc gctcgaatac gccgacgtcg aagtcggtga
     8641 ctatccgaat cgtgccgcgc ctagaagtct aggcggaacg tggcaccggc gccgctcggc
     8701 gcgatgaccg gcgtcaccgt gaagcgatcg cgacgaacgc gatggccgcg cgacgcatgc
     8761 tctcggctgc tcgatgggtt cttctcgatg aagcccttgt agaacgcgag gacggcgaag
     8821 ccgccggccg caatcgcgac gctgagaccc gtgtagccgt acagctggcc cttcgagccg
     8881 tggtcttccc attttttgaa gccggccgcg tcgagctgca tggacatcgg gacgtccttc
     8941 ttctccgggc acgccatgcc gtcgatcgag tgacccgcgt acgcgccgcc agcgcattcc
     9001 gctctcgctg cttcatcgcg catctggttg cccttgagac cgagcgcgat cgcgccgccg
     9061 ccaacgagca ccgtgccgat gaacacgccc ttccacatcg cggagccacg cgacgtctgg
     9121 tcgctggggc ccggacctgg acctgggcct ggccccggac ctggaccgac cacctcgccg
     9181 gtcacgccct tctccatctc gacggggatg ttctgcgtgc ccgacgtgac ggtgacgtct
     9241 ttctcccagc gatggaagcc gtcgctctcg acggcaacgc gaaccttgcc ctcgggaagt
     9301 cccgagaccg tgcccgtgcc gctgctgatc gagcccttct cttcgccgtt gatgaggatc
     9361 gtgccgcggt ctgcgttcgc gacccgaatc acgatcgtgc cgccggtcgc ttcaccggtg
     9421 agctttgcgt agagcttctt cgcccagcct tggagcgcgg cgcctgtcga ctccgacacc
     9481 ggaatcggat cggtcgtcgt cttcagcatc ttcttcgtgg cgacgtcgag gaccttcagc
     9541 gtcacgacgt actggttctt gtccttcttg aggttgccgt acatcagcac gtcagcaccg
     9601 agctgattgc cgatgcccga catgcacgcg aacgcttccg tgtcgcagtt gttcagcagc
     9661 ttctggtcga tgagctcttt gtcgctcgcc gttgccagtt ggtactggct cgtgccggcc
     9721 ttcgcgcgac tgcgcaggcc atcggttaga tccttggcga cctgcgtgtc ttgggcggtc
     9781 ggtgtgccac tcgggtccac gacttccagg cccaggatcg cgatcgactg ccgaggtgcg
     9841 gcgaacgccg gggacgcaaa gagcgtcagc agcgctaagc aagccagtga aaaagcacgg
     9901 gtcatgaggc tcctcagaag cggtagtata caccggccag gccccgaaaa cctggcttgc
     9961 cagaattggc caggctaccg tctagaggcc cgtatgcgga ccctatcggc gcggatcctc
    10021 ctcggttttg ctgcgctgac catcacgttc ggggcgatca ccggcaacat cgtctacaac
    10081 cagcacacgc tcgagagcta cgtcgagctg gtcaacaccg ggctcgtccc tgtctcccag
    10141 cagatccggg agctggcccg ccggtgcgac gacctccgcc agtacgtgag cgaaggcttc
    10201 gacaaggagt ccaacgtcga tagcgcgcgc taccggctcg agaacctgcg gctgcgccgc
    10261 gacaaaggct tcgccgacgt ccagaagttc ttcgcgacgt acgacgacat ggacaagctg
    10321 gactccaagc accagctccg caaagccgtc accgacctcg acgttccgat ccgcagcacg
    10381 aagccgctgt acgacaagct cgcgctcgcg ttccaacatg gcgagccgct cgagagcaat
    10441 gcggtgaatg aaggcgtggg cccgctgcgc gatgccgagc ggcagatctc gaatcaagcc
    10501 aactcgctcg cgacgacgat cgagcgcagc gtcgccgccg gcctgcgcga cctccaagag
    10561 cgcgagacgc agtcacgcca catcacgatc ttcctcggcg cggtcgcggt gctgctcggt
    10621 ctcttgatga cgacgtgggt cgtgatcgcg ctgcgccctc tgcgtcgcct gcagaacgct
    10681 gcgcgccgcg tcgccgccgg tgattacggc aatcgcatcg aggtgcgcgg tcctgcggag
    10741 gtcgcggatc tcgcacgcga gctcaacacg atggcgaccg ccgtcgaaga acgcgagcgc
    10801 gagctggtgc gctccgaacg actcgctgcg gttggcaaga tggccgcgat gatcacgcat
    10861 gaggtgcgca atccgctgtc gtcgatcggt ctcaacacgg agctgctcga agacgaagtc
    10921 gaggggaacg aagaagcccg cgatctcgtg cgtgccattc accgcgaagt cgaccgcctc
    10981 accgcgatca ccgaggagta cctcacgttc gcgcggctgc cgaaaccgaa gctcgctccc
    11041 gaggcgatca acccgctcgt tggagcgctc gccgcgttcg tgcgcggtga cctcgcagtg
    11101 aagaaggtcg atctgaaagt ggacctcgct ccgggcgatc cgatcgcgat catcgatgcc
    11161 ggccaggtgc gccagtgcct catcaatctc gtccgcaacg cgagcgaggc cctcggcgcc
    11221 aagggaagcg gaagcgtcac actgcgcacg cgctccgagg gcgaccgcat cgtgatcgag
    11281 gtcgaagacg acggtgtagg cattccgtcc gacgtgctcc cgcgcctgtt cgatccgttt
    11341 ttctcgacga aagaaggcgg cagtggcctc ggtctcgcgc tgacgcagca gatcgtgaaa
    11401 gaccacggcg gcgacctggg tgtgcaatcg acgctcggca aggggacgac gtttaccgtc
    11461 agcgtgcccg ccaaagccgg ttgatcctgt atcatcgcga tcgggcatgt ccgacaacga
    11521 cgacaagcgc cggagcgacc gcgaagcggt aacgctcttt gtcgagtacg aaggcgccga
    11581 cgatctgctc ggcgattaca ccgagaacct ctcgtcgggc ggcacgttcg tcgcgacgaa
    11641 tcgcgagctg ccgatcggca cgcagatcca gctcgtgctg tcgttccccg gtctgctcga
    11701 gccgatctcg atcgtcggca tcgtgcggtg gacgcgcggc aatcgcggcg ctggcgacgt
    11761 cgtcgagggc ggtgaagagg caggtgccgg catcgagttc gcacccggcc cggcgcgcga
    11821 tcagctcgca gcgatgatcg agaagatccg cgagcgcgat cccaagacgg tgtcgcgcgt
    11881 ctacaaagtg ctcgtcgtcg aggacaaccg ccacgtcgcg cagctgatcc aagacggtct
    11941 gcgcggctcg gcgcgccgcg agttcggcgg cggcgtctcg ttcgacgtgc gcaacgccga
    12001 ggacggccgc caagcaatcg agatcctccg caaggacacg ttcgacgcgc tcatcgtcga
    12061 tgtctacctg cccgtcgtcg acggcgcgaa ggtcatctcg acggctcgca cggatctcgc
    12121 gctcacgaac ctgccgat
//
LOCUS       DATLBZ010000008         4655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_751764, whole genome shotgun sequence.
ACCESSION   DATLBZ010000008 DATLBZ010000000
VERSION     DATLBZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751764"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VMZ53_00265"
     CDS             complement(<1..204)
                     /locus_tag="VMZ53_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26899.1"
                     /translation="MKLAASIEPPRSAARHSSEFAANPIIAEAVSSAVRPGPFRDGAS
                     AAAPFIVDGGYTASDGRDHDSRRA"
     gene            529..999
                     /locus_tag="VMZ53_00270"
     CDS             529..999
                     /locus_tag="VMZ53_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUS26900.1"
                     /translation="MGGLVVINVPRLDSPPLLGAALMVIAGVAWGIYSLRGRGIVRPL
                     VATAGNFVRCVPVAGVLVVVAFAMRGYASPKGIALAAASGAVASGLGYSLWYAAVPSL
                     GATRAAAVQLTVPVITSIAAVTMLGEMMTTALVVGGCAIVGGLALALLARPRRS"
     gene            complement(1051..2352)
                     /locus_tag="VMZ53_00275"
     CDS             complement(1051..2352)
                     /locus_tag="VMZ53_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HUS26901.1"
                     /translation="MKRIVKARGALDAQECAALREAQKLVIWRAYGCASFVEYMTREM
                     GYTERAALERLRVAKAVEEVPALGEALNLGALSFSGARELSRVVTPETQEEWIAAAQD
                     KNVREIEELVSGHKPGDRPTDAPDPALKTKILRHEVKLSVAELESRARKALARLRGSS
                     VDDSDFMEACFKAVLDQCEESQLATVAPSSKPMTAPMTAPMTAPDAATLLLVGGRRLT
                     DAEVERLVAPRYQIAVTTCVGCKRGWQHGATTSLLSPPELERTLCDCVDIGDVDDEVP
                     ARAKRSIPKAVKRQVLHRDGYQCRVPGCRATANIDCHHIVYFMLGGDAVPMNLLALCE
                     GHHLAVHAGSLAIAGDAVNPTFTWSKQSKFKVEMRVVECTAALRARGVAKKLIKAVIA
                     STRAHVGNLDLTAQQWIDIALTKLAEEERSTRAQEERATAR"
     gene            2359..2727
                     /locus_tag="VMZ53_00280"
     CDS             2359..2727
                     /locus_tag="VMZ53_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26902.1"
                     /translation="MHTLARLFVITSDGTRVRYSAPRSNFIARSIAQHHTHLLARDPL
                     LDPSRNITRISSLAIEALSLLRSFDARSTIEKFEALVLAERAKIANPRRETGCEKWRA
                     ENAAQRRMRDNERAFLIASD"
     gene            3299..4458
                     /locus_tag="VMZ53_00285"
     CDS             join(3299..3560,3560..4458)
                     /locus_tag="VMZ53_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611749.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HUS26903.1"
                     /translation="MAKFVRKRYPAEFKKRLVELVRSGGRSYSSLQSEFGVHEQTIRV
                     WARQAKIDAGQADGLTTDERTELARLRRQNAELLEEREILKKFGGLVRPGVELEPAEA
                     FRFVEANQAMHKIRKMCRALGVSHSGYYAWCEREPSERAKADAQLSLKIHAIHEQSRS
                     TYGVPRVHAMLAADEVFVGKKRVARLMKAAGLEGISRRKKYRSTRDKDARPAPDLVER
                     AFVATAPNQLWVADITYVPTWAGFLYLAVVLDVYSRRIVGWAMDTHLRAELVIDALNM
                     AVGQRQPSTVIHHSDQGCQYTSVAFGLRCREVGVKPSMGSVGDAYDNAMCESFFATLE
                     CELLDRRRFKTQVEARLAVFDFIEGFYNTRRIHSSLDYRSPVEYERQQLKAA"
BASE COUNT          771 a         1550 c         1488 g          846 t
ORIGIN      
        1 ggcacgacgg ctatcgtgat ctcggccatc gctggcagtg taccctccgt cgacgatgaa
       61 tggggccgcc gctgacgcgc cgtcgcgaaa cgggcccggc cgcacggcgc tgctcaccgc
      121 ctcggcgatg atcgggttcg cggcgaactc gctgctgtgc cgcgccgcgc tgcgcggcgg
      181 ctcgatcgat gcggcgagct tcacggcgat ccggcttgcg acgggcgcag ccgtgctggc
      241 gctgatcgtc gtggcgcggc gccgggctgc ggcgatgcgc ggcgatggta cgaagatggt
      301 gcgcggcgat ggtgccgcgg cggcgatgca cggcgatggt gccgcggcgg cgatgcacgg
      361 cgatggtgcc gcggcggcga tgcgcggcga tggtgccgcg gcggcgatgc acggcgatgg
      421 tgccgcggcg acgcgtggtc atgctgcgga gggggtgcgt ggcgatcgtg tgggttgctg
      481 cgcggcgagc ggccgacgct gcgacagtgg ctcggttggg cgctggcggt gggtgggctc
      541 gtcgtcatca atgtgccgcg attggattcg ccgccgctgc tcggtgcggc gttgatggtg
      601 atcgcgggcg tcgcgtgggg gatctactcg ctgcgtggtc gtgggattgt gaggccgctc
      661 gtcgcgacgg ctggcaactt cgtgcgctgt gtgccggtcg ctggggtgtt ggtcgtcgtc
      721 gcgttcgcga tgcgcggcta tgcgtcgccg aagggcatcg cgttagcggc ggcctccggt
      781 gcagtcgcct cggggcttgg ctactcgctc tggtacgcgg cggtcccctc tctcggcgct
      841 acgcgcgccg cggccgtgca gctcacggtt cccgtgatca cgtcgatcgc cgcagtgacg
      901 atgctcggcg agatgatgac gacggcgctc gtcgttggcg gttgtgcgat cgtcggcggt
      961 ctggcgctgg ccctgctcgc cagaccgcga cgttcttgac gcacgtgacg ccctcgcctt
     1021 ttcgcgcagc cccgcagccc cgcagcctcg tcaccgcgct gtcgctcgtt cctcctgcgc
     1081 gcgcgtcgat cgttcctcct ccgccagctt cgtgagcgcg atgtcgatcc attgctgcgc
     1141 cgtcagatcc agattaccca cgtgggcacg agtcgacgcg atcacggcct tgatcaactt
     1201 cttagcaacg ccacgcgccc gtagtgccgc agtgcactcg accacacgca tctcgacttt
     1261 gaacttgctc tgcttgctcc acgtgaacgt cggattgacg gcatcgcccg caatcgcgag
     1321 cgaccccgcg tgcacggcga gatgatggcc ctcgcatagc gcaagcaagt tcatcggcac
     1381 cgcgtcaccg ccgagcatga agtaaacgat gtggtgacaa tcgatgttcg cggtcgcgcg
     1441 gcatcccggc acgcggcact gatagccatc gcggtgaagc acctgacgct tcaccgcctt
     1501 cggaatactg cgcttggcac gtgcaggcac ctcgtcatcg acatcgccga tatcgacgca
     1561 gtcgcacagc gtccgctcga gctcgggcgg cgacagcagc gatgtcgtcg cgccgtgctg
     1621 ccagccgcgc ttgcaaccga cgcacgtcgt aacggcgatc tgatatcgag gcgcgaccag
     1681 ccgctcgacc tccgcgtcag ttaggcgacg accaccgacg agaagcagcg tcgctgcgtc
     1741 gggcgcggtc atcggcgcgg tcatcggcgc ggtcatcggt ttcgatgaag gcgcaacggt
     1801 cgcgagttgt gactcctcgc attggtcgag gacggccttg aagcacgctt ccatgaagtc
     1861 actatcgtcg acgctacttc ctcgcagccg cgccagcgcc ttgcgcgcgc gtgactcgag
     1921 ctcggccaca gacagcttca cctcgtgacg aagaatcttc gtcttcagtg ccgggtccgg
     1981 cgcatccgtc ggccgatcgc cgggtttgtg ccccgacacc agctcctcga tctcgcgcac
     2041 gttcttgtcc tgcgccgccg cgatccactc ctcctgcgtc tccggcgtta cgacgcggct
     2101 gagctcacgc gcccccgaga acgacaaagc gcctagattc aaggcctcgc cgagtgcagg
     2161 cacttcttcg accgccttgg cgacgcgcaa ccgctcgagt gccgcgcgct cggtgtagcc
     2221 catctcgcgg gtcatgtact cgacgaagct cgcgcatcca tacgcacgcc agatcaccaa
     2281 cttctgggcc tcgcgcaacg ccgcgcattc ctgtgcatcg agcgcgccgc gcgccttcac
     2341 gatccgcttc aaggtgcgat gcacacgctc gcacgtctgt tcgtgatcac ttctgacggc
     2401 actcgcgtgc gctactccgc cccacgttcc aacttcattg ctcgatccat cgcgcaacat
     2461 cacacgcatc tccttgctcg tgatccgctg ctcgatccat cgcgcaacat cacacgcatc
     2521 tcctcgctcg caatcgaagc tctctctctt cttcgatcgt tcgatgctcg ttctaccatt
     2581 gagaaattcg aggctctggt tctcgctgag cgcgcgaaaa tcgcgaatcc tcgtcgagaa
     2641 accgggtgcg agaaatggcg cgcggagaac gcagcacaac gccgtatgcg cgacaacgaa
     2701 cgcgcgtttc tgatcgcgag cgattgacac gtcggaattc agtcaacaca aatacgacga
     2761 acgaggagat ctctgcgcga gatttgtgga acgtgagatg cgtcgtgcgg tcgacgagag
     2821 gacggttcat tcgtttgcgg cgaacggata tgtgacgcga tgagcccacg tacgtttcga
     2881 tccagctcac gaaccactcg ctctcaacgc cgacaagcgg gcagtcgagc ggagagatga
     2941 tgactgcaat gtgcgactcg cgcatgggtc ggcggggtgg cgcggcgcgc gctggcggcc
     3001 cgttgaaggg ccgcgcgcgc cgcttcccgc cggcccgcgc tctgggcgaa gtgattcgtt
     3061 cttcggcgtc ccgccgtacg ggctacagcc gttggcaccg agcaacgatc cggctcgtcg
     3121 atgagctcct ccctgcacga ccgacgtgtt tcgattacct cgcgacagac gtcggtttcg
     3181 aaacctcgcg acccacgtgg gtttcgatca acacgcgacc cacgtgggtt tcgatcacct
     3241 cgcgacctga agttgccccg ctttcgtgga cacccgacta ccctaggagg agaccacgat
     3301 ggcgaagttt gtgcggaaga gatatccggc tgagttcaag aagcgacttg tcgagctcgt
     3361 tcggagcgga ggccgttcgt acagcagcct tcagtccgag ttcggagtgc atgagcagac
     3421 gattcgcgtc tgggcacggc aggccaagat tgacgccgga caagcagacg gcctcacgac
     3481 cgacgagcgc accgagctgg cccgtctgcg ccggcagaac gcggagctgc tcgaggaacg
     3541 agagatccta aaaaaattcg cggcctggtc cgcccaggag tcgaactgga acccgcggaa
     3601 gcgttccggt tcgtagaggc gaaccaggcc atgcacaaga ttcggaagat gtgccgcgca
     3661 ctcggtgtct cgcacagcgg ctactacgcg tggtgcgagc gcgagccgtc cgagcgcgcg
     3721 aaagccgatg cacagctgtc gctgaagatt catgcgattc acgagcagtc tcgcagcacc
     3781 tacggtgtgc cgcgcgtgca cgcgatgctc gcggccgatg aagtcttcgt cggcaagaag
     3841 cgcgtcgcga ggctgatgaa ggcagccggc ctcgagggaa tcagtcgtcg gaagaagtac
     3901 cgcagcaccc gggacaagga cgcgcggcca gcaccggacc tcgtcgagcg cgcgttcgtc
     3961 gcgaccgcac ccaatcagct ctgggtcgcc gacatcacat acgtgcccac gtgggcgggc
     4021 ttcctgtacc ttgccgtcgt tctcgacgtc tacagccgcc gcatcgtcgg ctgggcaatg
     4081 gacacgcatc tcagggccga gctcgtcatc gatgcgctca acatggcagt cggccagcgc
     4141 cagccctcga ccgtcatcca ccactcggac cagggctgcc aatacacctc cgtcgccttc
     4201 ggtctgcgct gccgcgaggt cggtgtgaag ccatcgatgg gctcggtcgg tgacgcctac
     4261 gacaacgcga tgtgcgagtc cttcttcgcg acgctcgaat gcgagctact cgaccgccgg
     4321 cgtttcaaga cacaggtcga agctcggctg gccgtgttcg acttcatcga aggcttctac
     4381 aacacgcgtc gcatccactc gtcgctcgac taccgctcgc ccgtcgagta cgaaaggcag
     4441 cagctgaagg ccgcataggt gagtgatcca acccgtccat caaagcgggg caacttcaca
     4501 ccacgtgggt ttcgatcacg cccatcgcct cgcgaccata ggccgcccct cgatagctag
     4561 cgagcgccct tgatcacgaa ctgatccgga agcgcgacgt cgaccacgct cgcgtgcagg
     4621 ccacccgctt cgagctcgcg cttcacttgc tccgc
//
LOCUS       DATLBZ010000009         8328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2985227, whole genome shotgun sequence.
ACCESSION   DATLBZ010000009 DATLBZ010000000
VERSION     DATLBZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2985227"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..2660)
                     /locus_tag="VMZ53_00290"
     CDS             complement(141..2660)
                     /locus_tag="VMZ53_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS26904.1"
                     /translation="MTDVVTDDDLDGDVDDADSDAFLREVARVDDLPPPDGELRAGDR
                     LAHFVIGARLGAGSMGAVYEARDEKLGRTVAIKVMRRTTDEARARFLQEARAAAAVSH
                     PNLVTVHEIGEDAGRAFIVMERIRGKTLRGVRASSAREAVDLLTAIAAGLAAAHAAGV
                     VHRDLKPDNVMRTEDGAIKVLDFGIARLTDDSALAIGTPPMTGTGLIVGTPSYMSPEQ
                     AAGEPVDARSDIYSFGVVARELIFGARAPKVPPGEPGSLPAILDRCVRDARDERYANG
                     REVHAALSTLSAKLEARRTPRSRSLVWLVCALVGLVGIGIAWRVTRSSTPPAAPRVKP
                     RALAAAADLRTTNTQARDAYVGAFTALHDAQFFEAVRGFERAVELDPGFAAAHLRLGL
                     AKRWFHSKEEGRTSFAFAVKHRDELDARDRGLLEAAEPAVMREPFDYAESQKRFSALL
                     AIYPDDTELMYWLGWTYGELGEHEQLMPLLDRMAAVDPTAAVVMSSRALTLFYLGDLR
                     GAREAAIECRRIAPTQGTCAFELQKILDDAGACDELETVLRAWMIASPDEPRAPHALA
                     RLLLATRRPREAVDEMFEQARSRLPEAQRAAAKLDDDDAIAFATGDFTQAPPDHGPVR
                     NALDRIDLAFETGNAQLARRTAEQFLAKSSAFSQGHGSSDGDLFGDPTGAILATLART
                     GAITREELQHKRDAWVHELDASIGGNYHRMIWAHGYADTVATADEAAGALAALPRYEP
                     LPATYWFPFVYGRIGRTFVLGGRPADAMPYLERATKRCMERDPRDFLYLGLAHQALGQ
                     SAEACSAFGEVVTRWGNAKPRSVTAEKARQQMAALACAPRG"
     gene            2690..3286
                     /locus_tag="VMZ53_00295"
     CDS             2690..3286
                     /locus_tag="VMZ53_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS26905.1"
                     /translation="MPASETVEREVRALCDAGDHAGAATAALRGYGPELYSFLAAIHR
                     DDADDVFAEVSAKIWRGLPAFGWQSSLRTWAYVIARNTSLTARTRGGREVPLAESEAE
                     KLAVEVRTATKTYLRTDAKTKFAEIRAALPIEDQELLILRVDRGLDWKELAQTLAGAD
                     LDDAALKREAARLRKRFQVLKDKLLEAGRAAGLVGTRS"
     gene            complement(3289..3855)
                     /locus_tag="VMZ53_00300"
     CDS             complement(3289..3855)
                     /locus_tag="VMZ53_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26906.1"
                     /translation="MTNTLTAFIAISIATVHVAAADSKAKPAPTKPTAAKPAEPKREP
                     TDAKPAQLIGRWEGTSEFKIRGKTTTWKGTGACERAAISPAITCAMVGVSGDMRIEEL
                     WMLGYDDRSATYHLFSTNSWGEAYDHAAKWSDASTVSFVHSATRDGKPLVETYTLTFK
                     GDQMIWHGVQKVGDEVIGDGTTTFKRVP"
     gene            complement(3897..5999)
                     /locus_tag="VMZ53_00305"
     CDS             complement(3897..5999)
                     /locus_tag="VMZ53_00305"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS26907.1"
                     /translation="MGGDDRDLGLSDTAQAPPPAAAKPLDATLDASVHPLAAGTAKPR
                     ERLSDTALASPSSSLGASRIGTAREVAFARATNVVLALLSTVHIAYFVVRFLVADSPP
                     ALHKAYIGRVNGGPFQAMDIFNGPFLTLARITLVATVFVAVSVWVARYHPKNRIMQLV
                     ASAVSVAGQSSVAVAVLTSQETLLAPRIVAAVIYVVMPLSGFTIIALFPNGKPVPRWA
                     LGLIPIGTVPFAIQAGIMFFTRGFSVPVAAACFPFGLVALAMMSHRYRTHATLREQHQ
                     IKWLAYAGIAFLLLQAIVIALIPLLMHPESPAFPFIRVLFELVLASSYLVALACMLFS
                     AARYRLWDIDRVINRTIVYVLVTAMLGAACVLGYFGLRAALSTLPAVVSIAISIGLMA
                     ALFAPARRRIGRFIDRKFYGIGLDYERLAAKAVHAARASLPTISTELGAYDELVLLGR
                     GGMGAVYRAHHPDFGVPVALKVMSPKLADDPDAQARFRREAQLLEDLQHPNVIPFLAA
                     GHENGLAFIAMQYVDGQDLSAVVRARRRLSLAEVVPIIDGVAAALDVAHEAGIVHRDI
                     KPANIFIEAGGKPLVMDFGVALLAGDARAADGSLVGSLPYIAPEQIHNADAVDRRADI
                     YALGATVYEMVTGRPPFVETTALGLVLAHLHQPPVDPRDHVPTLSSAASLAILKALSK
                     AASERFGTAGEFAAALRA"
     gene            6053..8107
                     /locus_tag="VMZ53_00310"
     CDS             6053..8107
                     /locus_tag="VMZ53_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS26908.1"
                     /translation="MRYVCPECGDGDDAAGACPVDGTQRSPVGADALLGTTIGSWRVA
                     RLLGAGGMGRVYVAVQPQIGARVAIKVLKRESAADPDVVDRFFNEARAVNMIKHEAIV
                     DVIDLGRLPDGAPYIVMEYLDGASLGAIVRREKRLALGTLSRIVSEVLAALGAAHAKA
                     IIHRDLKPDNVFVSPSGRVTVLDFGVAKLAADAEGFGSTQTGSLLGTPAYMSPEQARS
                     QPLDARSDLYAVGVMLYEAATGKLPFVAQNLFDLLQMHVAQAPRPPRELAPEIPDTFE
                     AVILRALAKDPKDRFASAAEMRAALATAADGLPQLALSSVVIPAEPPGHEKKSDPFAE
                     TAASDSIATDKTQVASAPAKPAESKQPAQAKVEPNPLEQRQGEAPRSIQLTRGTLFAL
                     LAAAALLGLGGAALVMAFGKRDAPTGVVSPPANVAADVHDASVIVDSQPAVAATTIDK
                     PVSRSVPEDAGGRSAVLTPVHDAGVAAVKYRPDAAAVSATTPADAAEVAATTPDAVAS
                     QWTMSKPKAGETKEETLERLLRSTEAIAMANPKAIDPLAEYKRAVARVVPLAGKAAFL
                     TSMRIHAANAQGLVDVTSEDGSVEIYFASPSLAKVGEPCYFVFIYEEGVARFNRAEQG
                     ENDKCNNGVRPPACSLAEIIKRAEAKGMVIGKKGVTFNRFPRGWMVSSGDYGDHIDDD
                     CE"
     gene            complement(8108..>8328)
                     /locus_tag="VMZ53_00315"
     CDS             complement(8108..>8328)
                     /locus_tag="VMZ53_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-2-methylbutyryl-CoA dehydrogenase"
                     /protein_id="HUS26909.1"
                     /translation="IRVITIAPGIFDTPMLGALPEEKRAALASDVVFPKRLGDPSEYG
                     ALVRAIVENGYLNGETIRLDGSLRMPPK"
BASE COUNT         1429 a         2697 c         2853 g         1349 t
ORIGIN      
        1 tgcggttccg gttgcggcgc cggctgcgca cctgcaaccg aagcgcacgc cagccccagc
       61 gcgacaacga tccacctcat ctcctcatta gaaccggatc gccgacgcgt gtcgccggca
      121 aaacgcgttc cgatcgtgag ctacccgcga ggcgcgcacg cgagcgctgc catctgttgc
      181 cgcgccttct cggcggtcac cgagcggggc ttggcattgc cccaccgtgt gaccacttcg
      241 ccaaacgcgc tgcacgcttc tgcgctctga ccgagcgcct gatgcgcgag ccccagatag
      301 aggaagtcgc gcgggtcgcg ctccatgcac cgcttcgtcg cgcgctcgag atatggcatc
      361 gcatcggcgg gtcggccgcc gagcacgaac gtccggccga tccggccata gacaaacgga
      421 aaccagtacg tcgccggcag cggctcgtag cgagggagtg cagcgagcgc gccggcggct
      481 tcgtccgctg tcgcgacggt gtcggcgtat ccatgcgccc agatcatgcg gtggtagttg
      541 cccccgatgc tcgcgtcgag ctcgtgcacc catgcgtcgc gcttgtgctg cagctcctcg
      601 cgcgtgatcg ctcccgtgcg tgcgagcgtc gcgagaatgg cgcctgtggg atcgccgaag
      661 agatcgccat cgctagatcc gtgcccctgc gagaacgcgc tgctctttgc caagaactgc
      721 tcggcggtgc ggcgagcgag ctgcgcgttg cccgtctcga acgcgagatc gatccgatcg
      781 agcgcattgc gcaccgggcc atggtcggga ggcgcctgcg tgaagtctcc ggtcgcgaat
      841 gcgatcgcgt cgtcgtcgtc gagcttcgcg gccgctcgct gcgcctcggg aagtcgcgac
      901 ctcgcctgct cgaacatctc gtcgacggct tcacgcggac gtcgcgtcgc gagtagcagc
      961 ctcgcaagcg catgaggagc gcgcggctcg tcgggactcg cgatcatcca tgcccgcagc
     1021 acggtctcga gctcgtcgca tgcgccggca tcgtcgagga tcttttgcag ctcgaacgcg
     1081 cacgtccctt gggtcggtgc gatgcgcctg cattcgatgg cggcttcgcg cgcaccgcgc
     1141 agatcgccta gatagaacag cgtgagcgcg cgcgatgaca tgacgaccgc cgctgtcgga
     1201 tcgaccgccg ccattcgatc gagcagcggc atcagctgtt cgtgctcgcc gagctcgccg
     1261 tacgtccacc cgagccagta catgagctcg gtgtcgtcgg gatatatcgc taacagcgct
     1321 gagaatcgtt tctgcgactc ggcgtagtcg aacggctcgc gcatgacggc aggctcggcg
     1381 gcctcgagca gaccgcgatc acgcgcatcg agctcgtcgc gatgcttcac cgcgaacgca
     1441 aacgacgtgc ggccctcctc cttcgaatgg aaccatcgct tcgcgagacc taggcgtagg
     1501 tgcgccgcgg cgaaccctgg atcgagctcg actgcgcgct cgaatccgcg cactgcttcg
     1561 aagaactgcg catcgtgcag cgcggtaaaa gctccgacgt atgcatcgcg cgcttgcgta
     1621 ttcgtcgtgc ggaggtcggc tgcagcggcg agagcgcgcg gcttcacgcg tggggcggcc
     1681 gggggcgtgc tcgaccgcgt tactcgccag gcgatgccga tgccgacgag cccgacgagc
     1741 gcgcacacga gccagacaag actccggctc cgcggtgtgc ggcgagcttc gagcttggct
     1801 gaaagcgtcg agagcgcagc atgcacctcg cggccgttcg catagcgctc gtcacgcgcg
     1861 tcgcgtacgc atcgatcgag aatggccggc aacgagcctg gctctcccgg cggtaccttc
     1921 ggagctcgcg cgccgaagat cagctcgcgt gcgacgacgc caaacgagta gatatcgctg
     1981 cgagcgtcga caggctcgcc agctgcctgc tcgggagaca tgtagctcgg cgtcccgacg
     2041 atgagcccgg tgccggtcat cggaggtgtc cctatcgcca gcgcggagtc gtcggtgagg
     2101 cgcgcgatgc caaagtcgag cactttgatc gcgccgtcct cggttcgcat cacgttgtcg
     2161 ggcttgagat cgcgatgaac gacgccagcg gcgtgagctg ccgcgaggcc cgcggcgatc
     2221 gccgtcaata ggtcgactgc ctcgcgtgca gacgacgccc ggacgccgcg gagtgtcttg
     2281 ccgcggatcc gctccatgac gatgaacgcg cggcccgcgt cttcgccgat ctcgtgcacg
     2341 gtgacgagat tcgggtgcga aacggcggct gcggcacgag cctcctggag aaaccgcgcg
     2401 cgggcctcgt cggtcgtccg gcgcatgact ttgatggcga cggtgcgacc gagcttctcg
     2461 tcgcgcgctt cgtagacggc gcccatgctc cccgcaccca gccgcgcgcc gatcacgaaa
     2521 tgcgcgagcc ggtcacctgc gcgcagctcg ccatcgggcg gcggtagatc gtcgacgcgc
     2581 gcgacctcgc gcaagaacgc atcggaatcc gcatcgtcga catctccatc gagatcgtcg
     2641 tctgtcacca catcggtcac gcgagactcc ttgttatcat cgacgcgcga tgcctgcgag
     2701 cgagaccgtc gagcgcgaag tgcgtgcgct gtgcgatgcg ggcgaccacg cgggcgctgc
     2761 gaccgcggca cttcgcggct atgggcccga gctctacagc ttcctcgccg cgatccatcg
     2821 cgacgatgcc gacgacgtgt tcgcggaggt cagcgcgaag atctggcgcg ggctgcccgc
     2881 gttcggatgg caatcgtcgc tccgcacctg ggcgtacgtc atcgcgcgca acacgtcgct
     2941 gaccgcgagg acacgcggag gccgcgaggt gccgctcgca gagagcgagg ccgagaagct
     3001 cgcggtcgag gtgcgcaccg cgacgaagac atatctgcgc accgacgcga agaccaagtt
     3061 cgcggagatc cgcgcggccc tgcccattga agatcaagag ctgctcatcc tgcgcgttga
     3121 tcgcgggctc gattggaagg agctcgcaca gacgctcgca ggcgctgacc tcgacgacgc
     3181 ggcgctcaag cgcgaagccg cccgcctccg caagcggttc caggttctca aggacaagct
     3241 cctcgaggca ggacgtgcgg ccggtctcgt cggaacgcgc agctgagctc acggcacgcg
     3301 cttgaacgtg gtcgtaccgt cgccgattac ctcgtcgccg actttctgaa cgccatgcca
     3361 gatcatctgg tcgcccttga acgtgagcgt gtatgtctcg acgagcggct ttccatcacg
     3421 cgtcgcggag tgaacgaacg agaccgtcga tgcgtcggac cacttcgccg catggtcgta
     3481 cgcttcgccc cagctgttcg tcgagaacag gtgatacgtc gcggagcgat cgtcgtaacc
     3541 gagcatccag agctcttcga tacgcatgtc gccgctgaca ccgaccatcg cgcaggtgat
     3601 ggccggcgaa atcgcagcgc gctcgcacgc gccagtcccc ttccaagtcg tcgtcttgcc
     3661 gcggatcttg aattcgctcg tgccctccca gcgaccgatc agctgcgcgg gctttgcgtc
     3721 ggtgggctcg cgcttcggct cagccggctt ggcagcggtg ggcttggtag gcgctggctt
     3781 cgccttggaa tcggcagcag cgacgtggac ggttgcgatc gagatggcaa tgaaggcggt
     3841 tagcgtgttc gtcatgggga ggcagactgg cctcgtgacg gatgtgacgc agttttttac
     3901 gcgcggagtg cagcggcaaa ttcacctgcg gttccgaagc gttcgctcgc agccttcgag
     3961 agcgccttca ggatcgcgag cgatgcggcg gacgagagcg tggggacgtg gtctcgaggg
     4021 tcgacgggcg gctggtgcaa atgcgcgagc acgagcccga gcgcggtcgt ctcgacgaac
     4081 ggtggccggc ccgtgaccat ctcgtagacc gttgcgccga gcgcatagat atccgcgcgc
     4141 cgatcgaccg catcggcgtt gtgaatctgc tcgggcgcga tgtacggcag gcttccgacg
     4201 agcgagccat cggctgcacg tgcgtcaccg gcgagcaatg cgaccccgaa gtccatgacg
     4261 agcggcttgc cgcctgcttc gatgaagatg ttggccggct tgatgtcgcg gtggacgatg
     4321 cccgcttcgt gggcgacgtc gagcgccgca gcgacaccgt cgatgatcgg cacgacctcc
     4381 gcgagtgaga ggcggcgacg cgcgcgaaca acggcgctga ggtcttggcc atcgacgtac
     4441 tgcatcgcga tgaaggcgag gccgttctcg tggcccgccg ccagaaacgg gatcacgttc
     4501 gggtgctgca gatcttcgag gagctgcgct tcgcgacgga accgcgcttg cgcgtctgga
     4561 tcgtcggcga gcttcggcga catgaccttg agcgcgaccg gcacgccgaa gtcgggatgg
     4621 tgcgcgcgat acacggctcc catgccaccg cgaccgagca ggacgagctc gtcgtaggcg
     4681 cctagctccg tcgagatcgt tggcagtgat gcacgggcag catggacggc tttggcggcg
     4741 aggcgttcgt agtcgagccc gatgccgtag aacttgcgat cgatgaagcg tccgatgcga
     4801 cgacgcgctg gtgcaaagag cgccgccatg agcccgatcg agatcgcgat gctgacgaca
     4861 gcgggcagcg tcgacaaggc ggcgcgtaga ccgaagtagc cgagcacgca cgccgcaccg
     4921 agcatggccg tgacgagcac gtacacgatc gtgcggttga tgacgcggtc gatgtcccag
     4981 aggcgatagc gtgcggcgga gaacagcatg cacgcgagtg cgacgagata cgacgatgcg
     5041 agcacgagct cgaacagcac gcggatgaac gggaacgccg ggctctcggg atgcatcagc
     5101 agcgggatca gcgcgatcac gatcgcttgc agcagcaaga acgcgatgcc tgcgtaggcg
     5161 agccacttga tctggtgctg ctcgcggagc gtcgcgtgcg tgcggtagcg gtgcgacatc
     5221 atcgcgagcg cgacgagccc gaacgggaag catgctgccg cgaccggcac ggagaatccg
     5281 cgcgtgaaga acatgattcc ggcttggatc gcgaatggga ccgtcccgat cgggatgagg
     5341 ccgagcgccc accgcggcac tggcttgccg tttggaaaca gcgcgatgat cgtgaagccc
     5401 gacagcggca tcacgacgta gatcactgcg gccacgatgc gcggtgcgag caacgtctct
     5461 tgtgatgtca gcacggcgac cgcgaccgac gattgcccgg ctaccgacac cgccgacgcg
     5521 acgagctgca tgatgcgatt cttcgggtgg tatcgcgcga cccagacgga cactgcaacg
     5581 aagaccgtgg cgacgagcgt aattcgcgcg agcgtcagga atggcccgtt gaagatgtcc
     5641 atcgcttgga atgggccacc gttgacccgg ccgatgtacg ccttgtgcag cgccggtggc
     5701 gaatcggcga cgaggaaccg cacgacgaag tacgcgatgt ggaccgtcga caggagcgcg
     5761 aggacgacgt tggtggcgcg cgcaaacgcg acctcgcgcg cggtaccgat gcgcgacgcg
     5821 ccgagcgacg atgaaggcga tgcgagcgcg gtgtcggaga gtcgctcgcg cggcttcgcg
     5881 gtaccggccg cgagcggatg caccgaagcg tcgagggtcg cgtcgagcgg tttcgcggca
     5941 gcaggtggcg gcgcctgggc ggtatcgctt agacccagat cccgatcgtc accgcccatt
     6001 cgttcgtacg gtacaggtcg cactccccgc ggcgcaacgc tatgttcggg ccgtgcggta
     6061 cgtgtgcccg gagtgtggtg acggtgacga cgcggcgggc gcctgccctg tcgacggcac
     6121 ccagcgatca cccgtcggag ccgatgcact gctcggcacg acgatcgggt cgtggcgcgt
     6181 cgcgcgcttg ctcggcgcgg gcgggatggg gcgcgtctat gtggcggtgc agccgcagat
     6241 cggcgcacgc gtcgcgatca aagtgctgaa gcgcgagtcg gctgcggatc cggacgtcgt
     6301 cgatcggttc ttcaacgagg cgcgcgccgt caacatgatc aagcacgagg cgatcgtcga
     6361 tgtgatcgac ctcggccgct tgcccgacgg tgcgccatac atcgtcatgg agtatctcga
     6421 cggcgcatcg ctcggcgcga tcgtacgtcg cgagaagcgg ctcgcgctcg gcacgctttc
     6481 gcgcatcgtc agcgaagtgc tcgctgcgct cggagcggcg cacgccaagg cgatcattca
     6541 tcgcgatctc aagcccgata acgtgttcgt cagcccgagc ggccgcgtga cggtgctcga
     6601 tttcggcgtc gcgaagctcg cggcagatgc cgagggattt ggctcgacgc agaccggcag
     6661 cctgctgggc acgcccgcgt acatgtcgcc cgagcaagcc cgctcgcagc cgctcgacgc
     6721 acgcagcgat ctctatgcgg tcggcgtgat gctctacgag gctgcaaccg ggaagctgcc
     6781 gttcgtcgcg cagaacctgt tcgacttgct gcagatgcac gtcgcgcagg cgccacgtcc
     6841 tccgcgcgag ctcgcgccgg agattcccga cacgttcgag gccgtgatcc tgcgcgcgct
     6901 cgcgaaggat ccaaaggatc ggttcgcgtc cgccgcagag atgcgcgcag cgctcgcaac
     6961 agcggcagat gggttgccgc agctcgcact gtcgtcggtc gtgattccgg cggagccacc
     7021 cggtcacgag aagaagtcgg atccgttcgc tgagaccgcg gcgagtgact cgatcgcgac
     7081 ggacaagaca caggtcgcat cggcccctgc gaagccggcg gagtcgaagc agcctgctca
     7141 ggcgaaggtc gagccgaatc cactggagca gcggcagggc gaagcaccac gcagcattca
     7201 actgacgcgg ggcacgctgt tcgcgctgct cgctgcggcg gcgttgctcg ggcttggcgg
     7261 cgcggcactc gttatggcgt ttggcaagcg cgatgcgccc accggcgtcg tatcaccacc
     7321 ggcgaatgtc gccgccgacg tccacgatgc gtcggtcatc gtcgatagcc agccggcggt
     7381 tgccgccacg acgatcgaca agccggtgtc gcgctcggtt cctgaagacg cgggcggtcg
     7441 atctgccgtc ctgacaccgg tgcacgatgc cggcgtggcg gcggtcaagt accgacccga
     7501 tgccgccgct gtgtcggcga cgacgcccgc cgatgcagcg gaggtcgcag cgacgacgcc
     7561 cgacgcggtc gcatcccaat ggacgatgag caaacccaag gcgggcgaga cgaaggaaga
     7621 gacgctcgag cggctgttgc gaagtaccga agcgatcgcg atggccaatc ccaaagcgat
     7681 cgatccgctc gccgagtaca agcgcgccgt tgctcgagtc gtcccgctcg cgggaaaggc
     7741 tgcgttcctg acctcgatgc gaatccacgc tgcgaatgcg caaggtctcg tcgacgtcac
     7801 gagcgaagac ggctccgtcg agatttactt tgcgtccccg agcctcgcga aggtgggcga
     7861 gccctgttac ttcgtcttca tctacgaaga aggcgtcgcc aggttcaacc gcgcggagca
     7921 gggcgagaac gacaagtgca acaacggcgt ccggccacct gcgtgctcgc tcgctgaaat
     7981 catcaagcgc gccgaagcga agggcatggt gatcgggaag aagggcgtta ccttcaatcg
     8041 cttcccacgc ggctggatgg tgtcgtccgg tgattacggc gatcacatcg acgacgactg
     8101 cgagtgacta cttcggcggc atgcgcagcg agccgtcgag tcgaatcgtc tcgccgttga
     8161 gatagccgtt ctcgacgatc gcgcggacga gtgcgccgta ctccgatgga tcgccgaggc
     8221 gcttcgggaa caccacgtcg gaggccagcg ccgcgcgctt ctcttcgggc agcgcaccga
     8281 gcatcggcgt gtcgaagatg ccaggtgcga tcgtgatgac gcggatgc
//
LOCUS       DATLBZ010000010         6108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6712257, whole genome shotgun sequence.
ACCESSION   DATLBZ010000010 DATLBZ010000000
VERSION     DATLBZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6712257"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VMZ53_00320"
     CDS             <1..1333
                     /locus_tag="VMZ53_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26910.1"
                     /translation="PQEYAAADDEEVADGDVLAEADEGDADLLNADGEGDASHERPAY
                     PDAVADSLDASAPEAAADFPPPAQFAAPVAHVADSLANVALPDEYAGDAPLPEEEPLP
                     PPDEFDPEAAAAFDRESAAEFDRELRRESQGGIPAVDEQQDFEPVATDYVAGAPGDYV
                     EPAAHEYVKRAAGEYIEPAGDYVEPAPAAIGYADRPSVSDFAARLDLGDDDPPEEPIA
                     PPPVAYQRQRRASSAGDFPDPPSSSYTFAEQFPDEALPQSTLDRVLAEAGEGEDFDEP
                     HAYGTAPPQPQAYRQAPPEPMHYEPPTVQPRGFDPAPEPPKRPSSDDLEDALQALDVD
                     LGDAGVPRERVRRPTPSSQRPLPGLPIQRPTGPVPLAPKPAATTTGPVRTVAHTVKRA
                     GTKPPTMPPPPPAAAFRKTPTQQVPVTPAPQQQPVRATTDDGIEIDFDEDE"
     gene            complement(1341..2687)
                     /locus_tag="VMZ53_00325"
     CDS             complement(1341..2687)
                     /locus_tag="VMZ53_00325"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HUS26911.1"
                     /translation="MHLVDEQEAVPVRTKDDLLAYFATAGKPREAWRIGSEHELIGVI
                     RSTGEAPPYEGPHGIGALLQAFAAKSGDPFVENGHVIAVQRKDAQITIEPGGQFELAA
                     RPIVHDAELVSDMRSYVQELAEHSQKLGLAWLACGLRPFGTRANIPWMPKARYDVMRD
                     YMPTVGSRGLDMMQRTATVQVNLDFSDEADAAAKMRCLYSVTPILTALWAASPIVDEK
                     VSGFQSYRSWIWRDTDNARAGLVPFVFDRDDVFDAYTEWALDVPLYFIYRSGYVRVPA
                     GLTFRTFMRDGYEGVRATDSDWALHLSTLFPEGRLKKFIEVRGCDCGSLEMIEALAPM
                     MRGLLYDTTARAAATALTAGLSYEERQRVADDVPRSGLKTRAGKHTLGELAKQLVAIA
                     RDGLSRVAPASVPLIAAVEDIASSGRTQSDRIIELWQKHAGNRAALIGALAHPGLA"
     gene            2756..3310
                     /gene="pyrE"
                     /locus_tag="VMZ53_00330"
     CDS             2756..3310
                     /gene="pyrE"
                     /locus_tag="VMZ53_00330"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HUS26912.1"
                     /translation="MNPKRARLLELLRTRSFQEREVTLSSGLKSNFYIDCKQVSLDAE
                     GASLIGELFHAVIEEVAPSAVAVGGLTLGADPLATATSIYSFHAGKPRSAFIVRKEPK
                     GHGTGQWIESTKLPEGSPVVILEDVVTTGAATLKAIERARLAGYNILHAVAIVDRLEG
                     GREAVEAECKLTTLFTRRDFLPDK"
     gene            3307..3912
                     /locus_tag="VMZ53_00335"
     CDS             3307..3912
                     /locus_tag="VMZ53_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26913.1"
                     /translation="MMRALLLVVLCACGAAAPKVRLTDQWPTKAPQDYDDATETWTRK
                     SSLRGQYQEVMELAATFKSPEWRAARAVRDADNRDLQGPQRDAFMAQAQAEAAGPYEV
                     ELMVTTWDRRENDLDRGKKSIWGVVLIDQNGTQIEPLEIVKDKRPAFTVRTDFPALGD
                     FATAYVARFPRTTPVLGDNVKKVRLRLYSVRGSVEVAWESE"
     gene            complement(3949..5466)
                     /locus_tag="VMZ53_00340"
     CDS             complement(3949..5466)
                     /locus_tag="VMZ53_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26914.1"
                     /translation="MPESVERMRLERIAREEAEAKANAEAAAIAAGGGTAEEARAYAQ
                     RVKAAAEARRVDEERLARERGIAAAEGSPSRVDEPSAERSPSTVVVEAAADRSPSHAV
                     EPSAERSPSHVVEPSTERSPSTVVVEASGERSPSPAVEPPQEAVVDAGHAMSRERATQ
                     TDAKQLVKQLMADALPPSASSALTPSPSSSSPPSSSSPSSSSPSSSSSPRSSPSSSLS
                     SSASTSSSSSSSSDAVPFAAVPMSSAAVTAPVAAPGVPLPASAERTDELEAKTTQFQR
                     PELDNPFVAKPSPAAPAPSAPVVATPVTAATPSAPVVGTPNAATIITSPVAVAEGERT
                     SVSVADLLTVQHTTDTTARVTAVPRTLITETPSSISPSSAASAVIDSSTASGETVSGS
                     MRDRPVAPSPATSEPSILIADLQAVHTAASAAAQKAASFAKPPADAASSSRELEVSQT
                     RRDAVAFSDDEEEFFKRAESHSVMHPKFESFDDLDDGYEPPKFWDRVFGRKKPKK"
     gene            5896..>6108
                     /locus_tag="VMZ53_00345"
     CDS             5896..>6108
                     /locus_tag="VMZ53_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26915.1"
                     /translation="MSLYWLSLVGGGSLVRGALRVVGGRFRRRGGFGDGGSAFGGAAC
                     IGGGGSVGGGALGSGLCVGARHRVAGC"
BASE COUNT          954 a         2092 c         2068 g          994 t
ORIGIN      
        1 gccgcaagag tacgcagcag ctgacgacga agaagtcgcc gacggtgacg tgctcgccga
       61 agccgacgaa ggcgacgccg acttgctgaa tgcggacggc gagggcgacg cctcgcacga
      121 gcgtcctgcg tatcccgacg ccgtcgcaga ttcgctcgac gcgagcgcgc ccgaggcagc
      181 tgcagacttc ccgccgccgg cgcagtttgc cgcgcccgtc gcccatgtcg ctgactcgct
      241 cgcaaatgtc gcgctccccg acgagtacgc gggcgacgcg cccctgcccg aggaggaacc
      301 gcttccgccg ccggacgagt tcgatcccga agcagccgcc gcattcgatc gcgaatccgc
      361 ggccgagttc gatcgcgagc tgcgtcgcga atcgcagggc ggcatccccg cggtcgacga
      421 gcagcaagac ttcgagcctg tagcgacgga ttacgtcgct ggcgcaccgg gcgattatgt
      481 cgagcctgca gcgcacgagt acgtcaagcg tgcagcgggc gagtacatcg agcctgcggg
      541 cgattacgtc gagcctgcac cggcggccat cgggtacgcc gatcgtccat cggtgtccga
      601 ttttgctgcg cgcctcgatc tcggcgacga cgatccgccg gaagagccga tcgcgccgcc
      661 gccggtcgcg taccagcgtc agcggcgtgc gtcgagcgcg ggcgattttc cggacccacc
      721 gagcagcagc tacacgttcg ccgagcaatt ccccgacgaa gcgctgcccc agtcgacgct
      781 cgatcgcgtg ctcgccgaag caggcgaagg cgaagacttc gacgagccgc acgcgtacgg
      841 cactgcaccg ccacagccgc aggcgtatcg ccaggctccg ccggaaccga tgcattacga
      901 accgccgacg gtgcagccgc gcggcttcga tccagcgccc gagccgccaa agcggccgtc
      961 gagcgacgat ctcgaagatg cgctgcaagc gctcgacgtc gatctcggcg acgccggcgt
     1021 tccgcgcgag cgggttcgtc gtcccacgcc gagcagtcaa cgtccgcttc ccggactgcc
     1081 gattcaacgc ccgacggggc ctgtgcctct cgcgcccaag cctgcagcga ccacgacggg
     1141 ccccgtgcgc acggtcgctc atacggtgaa acgcgctggc acgaagccgc cgacgatgcc
     1201 accgccgcca cccgccgcag cgttccgcaa aacgccgacc cagcaagttc cggtgacgcc
     1261 tgctccgcag cagcagccgg tgcgcgcgac gaccgacgac ggcatcgaaa tcgacttcga
     1321 cgaagacgaa taagaccggt tcaggccaga ccgggatgcg ccagggcgcc gatgagcgct
     1381 gcgcgattcc ccgcgtgctt ctgccacagc tcgatgatgc gatccgactg cgtgcgtccc
     1441 gacgatgcga tgtcctcgac ggccgcgatc aacggaaccg acgcaggcgc gacgcgagac
     1501 aggccatcgc gcgcgatcgc gacgagctgc ttggcgagct cgccgagcgt gtgcttaccc
     1561 gcccgggtct tcaggccgga cctgggcacg tcgtcagcaa cacgctggcg ctcttcgtac
     1621 gagaggccgg cagttagcgc ggtcgcggct gcgcgcgcgg tggtgtcgta gagcaggccg
     1681 cgcatcatcg gcgcgagggc ctcgatcatc tccagcgagc cgcaatcgca gccgcgcact
     1741 tcgatgaact tcttgaggcg gccctcgggg aacagcgtcg agagatgcag cgcccagtcg
     1801 ctgtcggtcg cgcgcacgcc ctcgtagccg tcgcgcatga acgtgcggaa cgtcagcccc
     1861 gcgggcacgc gcacgtagcc gctgcggtag ataaagtaga gcggcacgtc gagggcccac
     1921 tcggtgtacg cgtcgaacac gtcgtcgcgg tcgaagacga atggaacgag gcccgcacgc
     1981 gcgttatcgg tgtcgcgcca gatccacgag cggtaggact gaaacccgct gactttctcg
     2041 tcgacgatcg gcgacgcggc ccacagcgca gtgaggatcg gcgtcaccga gtagaggcag
     2101 cgcatcttcg ctgcggcgtc ggcctcgtcg gagaagtcga ggttcacttg cacggtcgcc
     2161 gtgcgctgca tcatgtcgag gccgcgcgag ccgacggtcg gcatgtaatc gcgcatcacg
     2221 tcgtagcgcg ccttcggcat ccacggaatg ttcgcgcgcg tgccgaacgg gcgcaggcca
     2281 cacgcgagcc acgcgagccc gagcttctgc gaatgctcgg cgagctcctg cacgtacgac
     2341 cgcatgtcgc tgacgagctc tgcatcgtga acgatgggac gcgcggcgag ctcgaactgg
     2401 ccgcccggct cgatcgtgat ctgcgcatcc ttgcgctgca ccgcgatcac gtgaccgttc
     2461 tccacgaatg gatcgccgga cttcgcggcg aatgcctgca gcagcgcgcc gatgccgtgc
     2521 ggcccctcgt agggcggcgc ctcaccggtg ctgcggatca caccgatcag ctcgtgctcg
     2581 ctgccgatcc gccacgcttc gcggggctta ccggcggtcg caaagtacgc gagcagatcg
     2641 tccttcgtac ggacaggaac tgcctcttgc tcgtcgacga ggtgcacgca ggttcgggga
     2701 ctctaggggg tgatcccgtg gacgtcaatc gatccacctg tggtagcgtc agactgtgaa
     2761 tcccaagcgt gctcgcctgc tcgagctgct tcgtacgcgc agtttccaag aacgcgaggt
     2821 caccctctcg agcggcctga agtcgaactt ctacatcgac tgcaaacagg tctcactcga
     2881 cgccgagggc gcctctctga tcggcgagct gttccacgcc gtgatcgagg aggtcgctcc
     2941 gagcgcggtc gcggtcggtg gcctcaccct cggcgcggat ccgctcgcta cagcgacgag
     3001 catctacagc ttccatgcag gaaagccacg ttctgcattc atcgtccgca aggagccgaa
     3061 gggccacggc acggggcagt ggatcgagag cacgaagctc cccgaaggct cgccggtcgt
     3121 catcctcgaa gacgtcgtga cgacgggcgc cgcgaccctc aaagcgatcg aacgcgcgcg
     3181 gctcgcgggc tacaacatcc tgcacgcggt cgcgatcgtc gatcgtctcg aaggcggccg
     3241 cgaagccgtc gaggccgaat gcaagctcac gacgctgttc acacgccgcg atttcctccc
     3301 cgacaaatga tgcgcgctct gctcctcgtc gttctctgcg cctgcggtgc tgccgcgccc
     3361 aaggttcgct tgaccgatca gtggccgacg aaagctccac aggactacga cgacgcgacc
     3421 gaaacctgga ctcgcaagag ctcgcttcgc ggtcagtacc aagaggtgat ggagctcgcc
     3481 gccacgttca aatcgcccga atggcgcgcc gctcgtgcgg tccgggatgc ggacaatcgc
     3541 gatctgcaag gcccgcagcg cgacgcgttc atggcgcaag cgcaggccga ggccgccggg
     3601 ccgtacgagg tcgagctgat ggtcacgacg tgggaccgtc gcgaaaacga cctcgatcgc
     3661 ggcaagaagt cgatctgggg cgtcgtgctc atcgatcaga acggcaccca aatcgagccg
     3721 ctcgaaatcg tgaaagacaa gcggcccgcc ttcacggtgc gcaccgactt cccggccctc
     3781 ggcgacttcg cgaccgcata cgtcgcgcgc tttccgcgca cgacgccggt cctcggcgac
     3841 aatgtcaaga aggtgcggct gcgcctctat agcgtgcgcg gttccgtcga agtcgcctgg
     3901 gaatccgagt aggcggccga cgcccgctcc gaggcgccag ccgacgctct acttcttcgg
     3961 tttcttgcgg ccgaagacgc gatcccagaa tttgggcggc tcgtaaccgt cgtcgaggtc
     4021 gtcgaacgac tcgaacttcg gatgcatcac gctgtgcgac tcggcgcgct tgaagaattc
     4081 ctcttcgtcg tcggaaaacg ccactgcatc gcgacgggtc tgcgagactt cgagctcgcg
     4141 cgacgacgac gccgcatcgg cgggcggctt cgcaaagctc gctgccttct gcgccgcagc
     4201 cgacgcagcc gtgtggaccg cctgcaaatc ggcgatgagg atcgacggct ccgaggtagc
     4261 gggcgagggc gcaacgggcc tatcgcgcat cgagccgctg accgtctcgc cgctcgcagt
     4321 cgacgagtcg atgaccgccg aggccgcgct gctcggcgaa atagaagacg gcgtctcggt
     4381 gatgagcgta cgcggcacgg ccgtcacgcg cgcggtggta tcggtggtgt gctgaaccgt
     4441 cagcaagtcg gccaccgaga cgctcgtgcg ttcgccttcg gccactgcga caggcgatgt
     4501 gatgatcgtc gccgcgttcg gcgtgccgac aaccggcgcg ctcggcgtgg ccgcggtgac
     4561 gggcgtcgcg acaaccggcg cgctcggcgc gggcgcggcg ggcgagggct tcgcgacgaa
     4621 tggattgtcg agctcgggtc gctggaactg ggtcgtcttc gcttcgagct cgtcggtgcg
     4681 ctccgcggac gccggcaacg gcacaccggg tgccgcgacc ggcgcggtaa cggcggccga
     4741 gctcatcggg acggccgcga atggcaccgc atcgctcgat gatgacgacg acgacgacgt
     4801 cgatgcggac gacgacaacg atgacgacgg cgacgagcgc ggtgacgacg atgacgacgg
     4861 tgacgacgat gacggcgacg atgacgatgg cggcgacgat gacgacggcg acggcgtcaa
     4921 tgcggacgac gcagatggcg gcagcgcgtc ggccatcaat tgctttacga gctgctttgc
     4981 gtcggtctgt gtcgcgcgct cgcgtgacat cgcgtgtccc gcatcgacga ccgcttcctg
     5041 cggcggctcg acggccggtg atggcgagcg ttcgccagaa gcttcgacga cgaccgtcga
     5101 tggcgagcgt tccgtagacg gctcgacgac gtgcgatggc gagcgttccg cagacggctc
     5161 gacggcgtgc gatggcgagc ggtcggcagc agcttcgacg acgaccgttg atggcgagcg
     5221 ttccgcagac ggctcgtcga cgcgcgatgg cgaaccttcg gcagcggcga tcccgcgttc
     5281 gcgcgcgagt cgctcctcgt cgacgcggcg cgcttcagcc gcggccttca cgcgctgcgc
     5341 atacgcccgc gcttcttcgg cggtgccgcc gcccgccgcg atcgctgcgg cttcggcgtt
     5401 agctttcgcc tccgcctctt cgcgcgcaat gcgctcgagg cgcatgcgct cgacggactc
     5461 gggcacgcgc gcttgttgag ctgcaagctc gcgctcggct gcgtcgcgct ccgccgcttg
     5521 tcgctctgct gcttcgcgct tggccgcgtc gcgctccgcc gctcggcgct gtgctgcttc
     5581 gcgctcggct gcgtcgcgct ctgccgcttg tcgctgtgcc cccagtcgct cgtctgcggt
     5641 gttggcagct gctgcttgtc gctctgccgc gtgtcgatcc gccgcttgtg gctcgtctgc
     5701 ggtgttggca ccggctgctt gtcgctcgat cgcttgtcga ccgtctgcgg tgttggcacc
     5761 ggctgcttgt cgctgtgcag cgagtcgctc gtctgcgctg ttcgcacctg cagcttgtcg
     5821 atcggtcgct tgtcgatcgt ctgcggtgct ggcacctgca gcttgtcgat cgcctgcggt
     5881 gctggcacct gcagcttgtc gctctattgg ttgtcgctcg tcggcggcgg ttcgctcgtc
     5941 cgcggcgcgc ttcgcgtcgt cggcggtcgc tttcgtcgtc gcggtggctt tggcgacggc
     6001 ggcagcgcgt tcggcggcgc ggcttgcatc ggcggcggcg gcagcgtcgg tggcggcgcg
     6061 ctcggcagcg gcctttgcgt gggcgcccgc catcgagtcg ccggctgc
//
LOCUS       DATLBZ010000011        15733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8202711, whole genome shotgun sequence.
ACCESSION   DATLBZ010000011 DATLBZ010000000
VERSION     DATLBZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15733
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8202711"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /gene="tgt"
                     /locus_tag="VMZ53_00350"
     CDS             complement(<1..818)
                     /gene="tgt"
                     /locus_tag="VMZ53_00350"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HUS26916.1"
                     /translation="MPKLFDLPTPGFSFELIATLGHARAGILHTPRGDIETPVFMPVG
                     TAGTVKAMTADELRAPPLDAKIILGNTYHLYLRPGLEVIGAAGGLHKFAAWDRPILTD
                     SGGFQVFSLAGINEIDDDGVTFRSHIDGSKHRLTPEVSMEIQRVLGSDIAMVFDQCPP
                     GDAEASVHEVALARTTAWAKRCRVAPHAQGQAVFGIVQGGIDVPRRMRHMAEITALDF
                     DGHALGGLAVGESVADTYRILDEVAHQLPAEKPRYLMGVGTPADLEKGIAAGID"
     gene            complement(846..1277)
                     /locus_tag="VMZ53_00355"
     CDS             complement(846..1277)
                     /locus_tag="VMZ53_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26917.1"
                     /translation="MRNALVVFLVLAMAAPAARADSDQDKWRAAFAGSVMITATGTTM
                     MLWGRNRIDTAEHALCTGDYATGCGHPAPTTSAEVDEYNDKGARGQTIGRVGFGVLVT
                     GLVLTGITGYKGFAGGKKEQSVAVAPSIGPHGAGASLTLRW"
     gene            complement(1285..2328)
                     /gene="queA"
                     /locus_tag="VMZ53_00360"
     CDS             complement(1285..2328)
                     /gene="queA"
                     /locus_tag="VMZ53_00360"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HUS26918.1"
                     /translation="MFSPSDYNFELPPELIAQEPAAARDESRLLHVQTDGTLTDHRFP
                     EIVDMLPREAVLIANDTRVIPARVLGHKASGGRVELLFLEAEPEGHAHGEHVWRCLAR
                     ARRPIRAGQFVQIDGTDISVELVSERAVDGSVLVKVPSDAIELLERVGHIPLPRYIQR
                     DDRETDRERYQTMFAKVPGAVASPTAGLHMTPAIVEALKQRGVSLATVTLHVGYGTFE
                     PIRVDDIRSHHMHRERYMIPAETAELVASGRPIVALGTTALRALESAATAPRTLHAGP
                     GTTELFVYPGSSHEWRVVDHLITNFHLPESTLLMLVCAFAGTDRVLAAYRHAVAARYR
                     FFSYGDAMLLHRV"
     gene            complement(2340..3872)
                     /locus_tag="VMZ53_00365"
     CDS             complement(2340..3872)
                     /locus_tag="VMZ53_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26919.1"
                     /translation="MRIGVLLALAPVAACGRIAFDPQVECGPLGGMGHPYANSQQGAD
                     GIATPFLICSEAQLAAIAEHTEHWGSTFRLGADLDLTRPVAMIGTDTTPFTGALDGRD
                     HVINHLRIDAPLEDLAGLFRYADGARFANLTFVDAELHGHDNIGVLAAVAIDPTIENI
                     HVQGRVFGNRGVGGVVGTIDCPTCTHLVATDVTADIEVVATASNVGGVFGIVEATPSM
                     GGVARIEGLVALGSVFASNGVVGGAIGYGEGIDLSRSTSSASVSTDGGDAGGLIADVA
                     DTTSVVRDSSASGQVSCTRGLCGGLSGIQEGETIGCHATGRIAVCTNECAGLIADAYG
                     MVSRSYATGDVLATQDVVGGLVALTGVGVRDSYATGVVTGRDRVGGLFGATIIGLTIE
                     RSYATGQVTATSAVGGLGGAGGTMAGSTLVLVDSFATGAASGNTPAGTVSTLVGLNSF
                     TVTTNAYWSAPCFNAAASCTALGTMVADASYFHTITNDPLTRWDFTNVWREVPGALPQ
                     LR"
     gene            3932..6427
                     /locus_tag="VMZ53_00370"
     CDS             3932..6427
                     /locus_tag="VMZ53_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS26920.1"
                     /translation="MRSFLFLALMSSTALAAPIADLAKDIDGDGTPDKVVLDSSGELQ
                     VSTKRGTEKVQLGAMTKAKLAAAVVKNVPTIIVRVAKTGDVATDGVIVQLAGAGWKQV
                     VRTPIGPQGVDGDYWIDLDARPEGIYRYQARPGAVRCDGLPAYTFAEGLNGTKFQRLS
                     KIPTGIPENASVINAKPEASAPASNPSLFRARFASHQPGAMDAAALAIPTEIDDGNVD
                     TSWREDLTASSGEGQFFTYVSRVPTAKATQLRIVTTKVKGNNRPQRLGVIGSAGAWHV
                     DVPDGAPGSAFTAELTQPISGCVTIIIEATYGAPKGETSIAELAVFAEDERNGGGEKQ
                     LAHAVSEGKDGWIIAKQELARRGAAGAAAIEAELSATKDHHARNRLFGVLVTLKDPAA
                     APLLAKAAKENELQGQELVNAIAALAGTGMAQELADIATTKGMSLDARVAAVRALRPT
                     DAKDRDVLVSLAGKGVREVRHATIENLMTLDVPTLVAAAQAQQDAKAAGDLWRAVTRR
                     AQKQPAERPPALAAMTAALDSATDYERRYRLVDGVATIGDQAALKSLAALLARMPDGA
                     DAGAFKHIAARAIAINPRPEAFDLISKLVTDGDAGVRLAALSALAGSSGGAEGAWHGA
                     VGVDGIDRILQTSLLTDTWPDVRRFSAMVLGGRCSRPGPAAALADSLARDKHVGVRGD
                     SLAALVDCKAPGTDELVRKLWDDGKAPLELRRRAVDLTVGLGDAALAQKLVGKFTQWR
                     GAAIESEAALALAQNAAYAIGRLAPPGAAPALQSALDDSAFPEIVGAAATGLGLLGKA
                     CPASAKTKLAELAKSDEQQVSSAAARAVAVCGK"
     gene            6463..6948
                     /locus_tag="VMZ53_00375"
     CDS             6463..6948
                     /locus_tag="VMZ53_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26921.1"
                     /translation="MHALTPSFRTSWFLVAIVTSVLATVAATRPRPTPAPSFSDAPPF
                     EDQLVPAASLQCEHWSQSFDLTAAMQTCSVWADLDTNERWMLGVRASIVEHHVVDVII
                     TEIEHDCDYCFDREAKGREDRARYTLFAINACMRTELSAATFPDDTCTLDTRWQIKSD
                     W"
     gene            7003..7554
                     /locus_tag="VMZ53_00380"
     CDS             7003..7554
                     /locus_tag="VMZ53_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS26922.1"
                     /translation="MRLGIWLVVLAGCRFGFSQGDDSPSMMTNDAAIADTDATADARL
                     DSTTSATCPPSYVTVGGQTSKYRGLTNSIVWLSAEQTCETDGTHLVIVDNAAELTAMT
                     NLIPGQNLWIGVTDRKTIGAFLAVTGPSAAYLPWDASEPDGSAAECVFIDSLTLKMAD
                     QGCGSGRRAVCECDGVPADPTSY"
     gene            complement(7558..15645)
                     /locus_tag="VMZ53_00385"
     CDS             complement(7558..15645)
                     /locus_tag="VMZ53_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS26923.1"
                     /translation="MARIILNPTSASAIEPKDRLAAARQVLPFLEQTNQPAKQAAALE
                     VIRTSDDTGAGEKTDIDRTLMRLYAIDLGDDAAAWTAGLRVLKADPADQDVRGQLAAM
                     SGQLGRDGEWAGELAGALTTLKSKGGTSGQIRAVATELAKIAGERLGDRPTAERAWIT
                     VLEVEPDAPDAFEMLTGMYRHDERWQDLRAMLERRAEVTIDQETRLSTLIQLAQLEEE
                     TLGDAGRAGAAYRRVLEIDASHAPAYVALDRLFSSNGNWKELEELLARQTDHVDSERA
                     VEIAFRRAQLFAHKLGDPTRAVDLLEDVLAKSRAHAGARDLLEELMTAPNAQIVMMRI
                     VRLLEPLYEQDRLWSDLVALLRAQRPMVTGTEAVELLSRIATIEEAELNGARTAFECW
                     LEVLKLEPAHERARVELARLAQYLGRWPEATAALEAAVAATPDTDPATRAALLAELAT
                     YYDTQVGDTERAITAYKRLLEADPSSPQTIRRAGSALARLYEESQNWKELRAITRQQA
                     EWAEDPGERRALLSRVAQLDEEKLGDRDASIATWRDVLGEQANDPGALHALERLYEST
                     GNWRDLVDVLRRKVEMATTQPEAIETLARIAQLHEQKLQEVDEAIAAWLEIVDRDTED
                     TRALNELARLYAGAERWADLLDVYERQAMLYDGPTHTDLQISIARLLGGPLSRPVEAL
                     ERWAGVLRSDPQHPIALEAVNKALDDLDLRVAAADILRPVYAATGQEQRLAQLSLLQA
                     EWTDDPATKLRALLEVERLREYRLGDKPGAFEVQLLALRYAATEPELQSVIADTERLA
                     GELGREADLIDVYVDVAPDVLDAEIQRRLYLDIADLARAVRRDNELAREYYQKVLDAT
                     PEDRRSLAALESIYRETQDDTRLVDILLRQADIAPDVDDKVGELVEAASLYVQLKRPD
                     DAISTWEQVLQIAPERADAIYALEGLYSEQGRWHDVVDLYERRLGFVTSVEEAVALRV
                     QLGDIHDKQLHDVEAAIENYSAALSGHARQPLALAALEQYLNDPDARVMAAEVLEPIF
                     VAQQRWPDLVRIYSIKLDGAQDPAERLKLTRYVARLYEEQLEDFENALKWYAKLFRES
                     PGDPNVREQLHRLAGVVENWDFVAQTYQQYLDDESSEHEDVRDVAIALGVIYERRVNN
                     VDAAYSAYRRALAIEPEEETARQKASARDLVSRLENMLARSQKWPELLQIYDDVAART
                     DEDLRREALVKRARLLETGIGDAAKAIEGWREVVNVTESGESPILEQGYREAVGELER
                     LFRTRGQWRDLVDLFEAQQARASDASNFEEVAALRLKLAEVYEQHLNDITAAIDQYDE
                     VISGRLLWDRAVNSLERLVIHDEHRERIASLLEPVYREQDWWQKLVVILDAKLEYVND
                     PATQVLTLHEIAELHEQRGGAIDLALAALARAWRIDVADDVALTKLLSLAGKLGAWDE
                     AARTIEDGASSTDGELAASLWMKSAEIHEKQRADLKRAIEAWRKVDENRGDDPVALHA
                     LDRLLALEGRVEELVKVVERRSEVTDDAGVRLVLLHRVAALYEEVLEDKPRAIAAYKN
                     VLAVDDSDLAGLDALERLYRDTGDAREMVVTLERKIELTTDLPLRQELRLAAAQVYEH
                     HLDDVYQAIGQLSSVLDDDAGDLRALGELDRIYAKQKMWPELLEIVDKRALLATSQQD
                     RADYAYRSAHIVEVQLTDPDAAIPRYGAVLQVMPAHAEARTALENLMAKDDHVEAVTP
                     ILERVYRGERDAAGLVRVYERRLAVQGRDPSARLADWTALAEVREQIANQPTQAFVVW
                     GRALADDPANADLLEPLLRLAESQGLWREMAGLLEERLADDAPSLPPDVEQAYAMRLG
                     DICEDKLNDMDRASLAFGRASQGPEPKAALGALERVLSRASRWPDLAHVLRRQADVSD
                     DDLTKADYLYRLGELQETTLGSAKQAVAAYREVLELTPDHAQSRAALERLLSKHADLS
                     SEIVEILEPLLENDGDATRLVTIFEAKLNVVDDPLDKASILQRLVELAEGKLNDRARA
                     LDAALRWLAVDPASTQALSETERLSDRLGQWRETASRVDSIVHAKDAKERPDDVQVGL
                     LVFLGKVLRERLGQNEDAISTYRTALQLEPDSLAALDPLIALLRQRGEWVALADALRQ
                     RGRIAQELPEKRAAYAEVAQLSERAGDRDGAIEAWREIIDADESDRDALDQLARIYRT
                     AGHDPVALIEVLGKAARLAPNADDEKALRVEIAQLETEGPRGVAAWQAVVDLDPDDPN
                     ALQALEAAHARNSDWIAVSDIQMRRLDLAKTAQEKVLIQAEIARLAETRRNSVDDAIA
                     AWYGALDLDNSYVRAYDELERLLGSSARHHDLVELLDKRAELHAIQGNMSAELGALAR
                     AADVWEGKLDNPDAAGEILEKILAREPGSVAALTRLSKIYERAGDWDKCKATLEQALK
                     LNPQGRDAADLFFRLGEVARVGDTDDDTAIQHFQHALRYDAGHAGAITALEKLARARR
                     DNALLADMLGRRIAAITVPPERLALLVELAELERKAGRNDAALDALAKAQKEAPDDVR
                     VLSPLADMYFAAGRLDEAAPIYDKLATEAKAARRMKDVAKFRQRQGGILEARGDRSGA
                     LAAYEEALRVNPTDVGTMTGLGRMYFAGGDWEKARKIYQSLVLQNIDADAGVTKGEVY
                     WALGKIHIELGQAPKAKSMFQRGLELEPANQKLREALSQLQ"
BASE COUNT         2543 a         5357 c         5169 g         2664 t
ORIGIN      
        1 atgtcgatgc cggccgcgat gcccttctcg aggtccgccg gcgtgccgac gcccatcaag
       61 tagcgcggct tctcggccgg cagctggtgc gcgacctcgt cgaggatccg gtacgtgtcc
      121 gcgacgctct cgccgacggc aagtccgccg agcgcgtgac catcgaaatc gagcgccgtg
      181 atctccgcca tgtgacgcat gcgccgcggg acatcgatgc cgccttgcac gatgccgaac
      241 accgcctgcc cttgcgcgtg cggcgcgacc cggcagcgct tcgcccatgc cgtcgtgcgc
      301 gcgagcgcga cttcatgcac cgatgcctcg gcatcaccgg gtgggcactg atcgaagacc
      361 atcgcgatgt ccgatccgag gacgcgctgg atctccatcg agacctcggg cgtcagccgg
      421 tgcttgctgc catcgatgtg cgatcgaaac gtgacgccgt cgtcgtcgat ctcgttgatg
      481 cccgcgagcg agaacacctg aaagccgcca ctgtccgtca ggatcggccg gtcccacgcc
      541 gcaaacttgt ggaggccgcc cgctgcgccg atcacctcga ggccgggccg cagatagagg
      601 tgatacgtgt tgccgaggat gatcttcgcg tcgagcggcg gcgcgcgcag ctcgtcggcg
      661 gtcatcgcct tgacggtgcc cgcagtgccg accggcatga acacgggcgt ctcgatgtcg
      721 ccgcgcggcg tgtgcaggat gccagcgcgt gcgtgaccta gcgtcgcgat cagctcgaac
      781 gagaatccag gcgtcggtaa gtcgaacagc ttcggcacgc gccgacgagg tctatcagac
      841 ttcagctacc agcgcagcgt cagcgacgcg cccgcgccat gcggcccgat cgacggcgcg
      901 accgcgacgc tctgctcttt cttgccgccg gcaaagccct tgtagccggt gatgcccgtg
      961 agcacgagcc cggtgacgag cacgccaaag ccaacgcggc cgatcgtctg accgcgagcg
     1021 cccttgtcgt tgtactcgtc gacctcggcg gacgtcgtcg gtgcggggtg cccgcaaccc
     1081 gtcgcgtagt cgccggtgca gagcgcgtgc tcggcggtgt cgatgcgatt gcggccccac
     1141 aacatcatcg tcgtgcccgt cgccgtgatc atcaccgagc ccgcgaaggc tgctcgccac
     1201 ttgtcttgat cgctatcggc gcgcgcggcg ggagccgcca tggccaacac cagaaacacc
     1261 accagtgcgt ttctcatccg gcgatcatac gcgatgaagg agcatggcgt cgccataact
     1321 gaagaaacga tagcgggcgg cgaccgcatg gcgatatgcc gcgagcacgc ggtccgtccc
     1381 agcaaacgcg cagacgagca tcagcagcgt cgattccggc agatgaaagt tcgtgatcaa
     1441 atggtcgacg acgcgccact cgtgtgacga ccccggatac acgaacagct cggtcgtccc
     1501 gggtcccgca tgcagcgtgc gcggcgcggt cgcagcactc tcgagcgcgc gcaatgcggt
     1561 cgtcccgagc gcgacgatcg gccgcccact cgcgacgagc tccgcggtct ccgccggaat
     1621 catgtagcgc tcgcggtgca tgtgatggct gcgaatgtcg tcgacgcgga tcggctcgaa
     1681 cgtgccgtag ccgacgtgca gcgtgaccgt cgcgagcgac acgccgcgct gcttcagcgc
     1741 ctcgacgatc gccggcgtca tgtgcagccc cgccgtcggt gacgcgaccg cgccgggcac
     1801 cttcgcgaac atcgtctggt accgctcgcg atcggtctca cgatcatcgc gctggatgta
     1861 gcgcggcagc ggaatgtggc cgacgcgctc cagcagctcg atggcgtcgc tcggcacttt
     1921 cacgaggacc gagccatcga ccgcacgctc gctgacaagc tcgacggaga tgtcggtgcc
     1981 gtcgatctgg acgaactgcc ccgcgcgaat cggccggcgc gcccgcgcta aacaccgcca
     2041 cacgtgctcg ccgtgggcat gcccctccgg ctccgcctcc aagaacagca gctcgacgcg
     2101 accgccgctc gccttgtggc cgaggacgcg tgctggaatc acgcgcgtgt cgtttgcgat
     2161 gagcaccgct tcgcgcggca gcatgtcgac gatctccgga aaccgatgat cggtgagcgt
     2221 gccgtcggtc tgcacgtgga gaagccgcga ctcgtcgcgc gcggcagccg gctcttgcgc
     2281 gatgagctcg ggcggcagct cgaagttgta gtccgacggc gaaaacacgc gctcggagct
     2341 taccgaagct gcggcagtgc gcctggcact tcgcgccaca cattcgtgaa gtcccaacgc
     2401 gtgaggggat cgttcgtgat cgtgtggaag tagctcgcgt cggcaaccat cgtcccgagt
     2461 gccgtgcacg aggccgcggc attgaagcag ggcgccgacc agtaggcatt ggtcgtgacg
     2521 gtgaacgaat tcaggccgac gagcgtcgac acggttccgg ccggggtgtt tccactcgcc
     2581 gcgccggtcg cgaaggagtc tacgagcacg agcgtcgaac ccgccatagt accgcccgct
     2641 ccgccgagac ctcctaccgc cgacgttgca gtgacctgac ccgtcgcata cgagcgctcg
     2701 atcgtcaggc cgatgatcgt ggcgccgaac agcccaccta ctcgatcgcg accagtcacg
     2761 acgccggtcg cgtacgagtc gcgaaccccg acgccggtca acgcgacgag cccgccgacc
     2821 acgtcttgcg tggcgagcac gtcgccggtc gcgtagcttc gagacaccat cccgtatgca
     2881 tcggcgatga gtcctgcgca ctcattcgtg cagaccgcga tccggcccgt cgcgtgacac
     2941 ccgatcgtct cgccctcttg gatcccggac aggccgccgc acaaaccgcg tgtacacgac
     3001 acctgtcccg atgcgctcga gtcccggacg accgacgtgg tgtcggcgac gtcggcaatg
     3061 aggccgcccg cgtcaccgcc gtccgtgctg accgatgcac tcgacgtcga tcgcgatagg
     3121 tcgatgcctt cgccatagcc gatggcacct ccgacaacgc cgttactggc gaacaccgag
     3181 cccagcgcaa cgagtccctc gatgcgtgcc acgccgccca tgctgggcgt tgcctcgacg
     3241 ataccgaaca cgccaccgac gttcgacgcc gttgcgacca cctctatatc cgcggtcacg
     3301 tcggtcgcga ccagatgcgt gcaggtaggg cagtcgatcg ttcccaccac gccaccgaca
     3361 ccgcgattgc cgaacacgcg accttggaca tggatgttct cgatcgtcgg atcgatggcg
     3421 acggccgcga gcacgccgat gttgtcatgc ccgtgcagct cagcgtcgac gaacgtgaga
     3481 tttgcgaagc gcgcaccgtc ggcgtagcgg aacagccccg ccagatcctc gagcggagcg
     3541 tcgatgcgga ggtggttgat cacgtgatcg cggccgtcga gcgccccggt gaacggcgtg
     3601 gtgtccgtcc ctatcatcgc gacgggcctc gttagatcga gatccgcgcc gagccgaaac
     3661 gtgctgcccc agtgctcggt gtgctccgcg atcgccgcga gctgagcctc gctgcagatg
     3721 agaaacgggg tagcgatccc atcagcgcct tgctgcgagt ttgcataggg atggcccatg
     3781 cccccgagcg gcccgcactc gacctgcggg tcgaaggcaa tgcgaccgca cgccgcaacc
     3841 ggcgccagtg ccagcagtac gccgattcgc acgtcgcgag cttagctgca atccggctcg
     3901 ggccgacgtg tcctgcgcta tggtgaggtc cgtgcgcagc ttcctgttcc tcgcgctgat
     3961 gtcgtcgacg gcgctcgcgg cgcccatcgc ggacctcgcg aaggatatcg atggcgatgg
     4021 cacgccagac aaggtcgtgc tggacagcag cggagagctg caggtctcga cgaagcgggg
     4081 gacggagaag gttcagctcg gcgcgatgac gaaggcaaag ctcgccgcgg ctgttgtgaa
     4141 gaacgtgccg acgatcatcg tgcgtgttgc aaagaccggc gacgtcgcga ccgacggcgt
     4201 gatcgtgcag cttgccggcg ctggctggaa gcaggtcgtt cgcacgccga tcggaccgca
     4261 gggcgtcgac ggcgactatt ggatcgatct cgatgcgcgt cccgagggca tttaccgcta
     4321 tcaggcgcgt cccggcgcgg tgcgctgcga tggcttgccc gcgtatacgt tcgccgaagg
     4381 tctcaacgga acgaagtttc agcggctatc gaagatcccg acgggaattc cggagaacgc
     4441 ctcggtgatc aacgcgaagc cggaagcatc ggcgcccgcg tcgaacccga gcctgtttcg
     4501 cgcgcgcttc gcatcgcatc aacctggagc gatggatgcc gcggctctcg cgattcctac
     4561 cgaaatcgac gacggcaacg tcgatacgag ctggcgcgag gatctgactg cgagctccgg
     4621 cgaaggacag tttttcacgt acgtctctcg cgtcccgacg gcgaaggcta cgcagctgcg
     4681 gatcgtcacg acgaaggtga agggcaacaa tcgcccgcaa cgcctcggcg tcatcggatc
     4741 tgcgggcgcg tggcacgtcg atgttcccga cggcgctccg ggctctgcat tcaccgcaga
     4801 gctgacacag ccgatttcgg gctgcgtcac gatcatcatc gaagcgacgt acggcgcgcc
     4861 gaagggcgag acatcgattg ccgagctcgc ggtcttcgcc gaggacgagc gcaatggcgg
     4921 aggcgaaaag caactcgcac acgcggtgtc cgagggcaaa gacggctgga ttatcgcgaa
     4981 gcaggagctt gctcgccgag gtgccgcggg cgccgccgcg atcgaagcgg agctgagcgc
     5041 gacgaaggat caccacgcgc gcaaccggct attcggcgtg ctcgtgacat tgaaggatcc
     5101 cgcagcagcg ccgctgctcg cgaaggcggc gaaagagaac gagctgcaag gccaggagct
     5161 ggtcaacgcc atcgcggcac tcgcaggcac cgggatggcg caagagcttg ccgacatcgc
     5221 cacgacgaag ggcatgtccc tcgatgcgcg cgtcgcagcc gtgcgcgcgc tgcgtccgac
     5281 cgacgcgaaa gatcgcgatg tcctcgtctc gctcgccggt aaaggcgtgc gtgaagtccg
     5341 ccacgccacg atcgaaaatc tgatgacgct cgacgtgccc acgctcgtcg ccgccgcgca
     5401 agcacagcag gacgcgaagg ccgccggcga cctgtggcgt gccgtcacgc gtcgcgcgca
     5461 gaagcaaccc gcagagcggc cccctgccct cgccgctatg accgcggcac tcgattccgc
     5521 gacggattac gagcggcgat atcgcctcgt cgatggtgtc gcgacgattg gtgatcaagc
     5581 agcgctgaag tcgctcgcgg cgctactcgc gcgcatgccc gatggcgctg acgctggcgc
     5641 gttcaagcac attgctgcgc gtgcgatcgc gatcaacccg aggccggaag cattcgatct
     5701 gatcagcaag ctcgtcaccg acggtgatgc gggcgtgcga ctcgctgcac tctcggcact
     5761 cgctggatcg agcggcggcg cggaaggcgc gtggcacggc gcagtcggcg tcgatggcat
     5821 cgatcggatt ctgcagacgt cgctgcttac tgacacgtgg cccgacgttc gccggttctc
     5881 cgcgatggtg ctcggtggcc ggtgctcgcg tcccggaccg gcggccgcac tcgcggactc
     5941 gctcgcgcgc gacaagcatg tcggcgtgcg cggtgattcg ctcgcagcgc tcgtcgactg
     6001 caaggcgcct ggcaccgacg agctcgtgcg caagctgtgg gacgacggca aagcgccgct
     6061 cgagctgcgc aggcgcgcgg tcgatctcac ggtcgggctc ggcgatgcgg cgctcgcgca
     6121 gaagctcgtt ggcaaattca cgcagtggcg cggcgctgca atcgagagcg aggctgcact
     6181 cgcgctcgcg caaaacgcgg cgtatgcgat cggccggctc gctcctcccg gcgctgcccc
     6241 cgcgctgcaa tccgcacttg acgattcggc gttccccgag atcgtgggcg ctgccgcaac
     6301 gggcctcggg cttcttggca aggcgtgtcc cgcgtcggcg aaaacgaagc ttgccgaatt
     6361 ggcaaagagt gacgagcagc aagtgtcgag tgcagccgcg cgcgccgtcg cagtctgcgg
     6421 caagtagcgc aacccgcagc gcgccgctgt gtcgttccga cgatgcatgc gcttacgcca
     6481 tcgttccgca cgtcgtggtt cctcgtcgca atcgtcacga gcgtgctagc cacggtcgct
     6541 gccacgcggc cccgcccaac gcccgcgccg tcgttctcgg acgcgcctcc gttcgaggac
     6601 cagcttgttc ccgcggccag cctgcagtgc gaacactggt cgcagagctt cgacctgaca
     6661 gccgcgatgc agacctgcag cgtgtgggcg gatctcgata cgaacgagcg ttggatgctc
     6721 ggcgtccgcg cgtccatcgt cgaacatcac gtcgtcgatg tgatcatcac cgagatcgag
     6781 cacgactgcg actactgctt cgaccgcgaa gccaagggcc gcgaggaccg cgcgcgctac
     6841 acgctcttcg cgatcaacgc gtgcatgcgt accgagctct ccgcggcgac attccccgac
     6901 gacacctgca cgctcgacac gcggtggcag ataaagagcg actggtaggc ctccgaaacg
     6961 ctcgcatgcg tcgcgcggca acatcagtta cgatggtctg ccgtgcggtt gggcatttgg
     7021 ttggtcgttc tcgccggatg ccgctttggc ttcagccaag gtgatgactc gccgtcgatg
     7081 atgacgaacg acgccgcgat cgcagatacc gacgcgaccg ccgatgcacg actcgattcg
     7141 acgacgtccg ccacgtgtcc gccgagctac gtcaccgtcg gagggcaaac gtcgaagtac
     7201 cgcggcctga cgaacagcat cgtctggctc tcagccgagc agacctgcga gacggatggc
     7261 acccatctcg tcatcgtcga caacgcagcc gagctcaccg cgatgaccaa tttgattccg
     7321 gggcagaacc tgtggatcgg cgtcacggat cgcaagacga tcggcgcgtt cctcgcggtc
     7381 acggggccga gcgctgcgta tctgccatgg gacgcgagcg agcccgacgg aagtgcagcg
     7441 gagtgcgtct tcatcgattc gctcacgttg aagatggcag accagggctg cggctctgga
     7501 cgccgcgcgg tgtgcgagtg cgacggtgtg cctgcggatc cgacgagcta ttagcgccta
     7561 ctgcagctgc gacagcgctt cgcgcagctt ttgattcgcc ggctcgagct cgagaccgcg
     7621 ctggaacatg ctcttggcct tcggcgcctg acccagctcg atgtggatct tgccgagcgc
     7681 ccagtagacc tcgcccttcg tgacaccggc gtcagcgtcg atgttctgca acacgagcga
     7741 ctgatagatc ttgcgcgcct tctcccaatc gccgccggcg aagtacatcc ggccgaggcc
     7801 ggtcatcgtc ccgacatccg tcgggttcac gcgcaacgcc tcctcgtacg cagcgagcgc
     7861 gcctgaccga tcgccgcgtg cttcgagaat gccgccctgg cgttgccgga acttcgcgac
     7921 gtccttcatg cgacgcgcag ccttcgcttc ggtcgcgagc ttgtcgtaga tcggcgccgc
     7981 ttcgtcgagg cgaccggccg cgaagtacat gtctgcaagc gggctgagga cgcggacgtc
     8041 gtcgggcgct tccttctgcg ccttcgcaag agcgtcgagc gcggcgtcgt tgcgacctgc
     8101 cttgcgctct agctccgcca gctcgacgag tagcgcgagt cgctctgggg gcaccgtgat
     8161 cgcggcgatg cggcgtccga gcatgtcggc gagcagcgcg ttatcccggc gggcgcgcgc
     8221 gagcttctcg agcgcggtga tcgcgcccgc gtggccggcg tcgtagcgga gtgcatgctg
     8281 aaagtgctgg atcgccgtgt cgtcgtcggt atcaccgaca cgcgcgacct cgccgaggcg
     8341 gaaaaacaga tcagcggcgt cgcggccctg cggattgagc ttgagcgctt gctcgagcgt
     8401 tgccttgcac ttgtcccaat cgcccgcgcg ctcgtagatc ttcgacagcc gcgtgagcgc
     8461 agcgaccgag ccaggctcgc gcgcgaggat cttctcgagg atctcgcccg cagcgtcggg
     8521 gttgtcgagc ttgccttccc acacgtcggc agcacgcgcg agggcaccga gctccgcgga
     8581 catgttgccc tggatcgcgt ggagctcggc gcgcttgtcg agcagctcga cgagatcgtg
     8641 gtggcgcgcc gaagaaccga gcaaccgctc gagctcgtcg taggcacgga cataggagtt
     8701 atcgagatcg agcgcgccat accaagccgc gatcgcatcg tcgacggagt tgcgacgcgt
     8761 ctcggcgagt cgagcgatct ccgcctgaat gagcaccttc tcctgcgcag tctttgcgag
     8821 gtcgaggcga cgcatttgga tgtcgctcac cgcgatccag tcgctgttgc gcgcgtgggc
     8881 agcttcgagc gcctgcagtg cgttcgggtc gtccggatcg agatcgacga ccgcctgcca
     8941 tgcggccacg ccgcgtggac cctcggtctc cagctgcgcg atctcgacgc ggagcgcctt
     9001 ctcgtcatcg gcgttcggcg cgaggcgtgc ggctttgccg aggacttcga tgagcgcgac
     9061 cggatcatgg ccggcggtgc ggtagatgcg cgcgagctga tcgagcgcat cacgatccga
     9121 ctcgtcggca tcgatgatct cgcgccacgc ttcgatggca ccgtcacgat caccagcgcg
     9181 ctcggacagc tgcgcgacct cggcatacgc cgcccgcttc tcgggaagct cctgggcaat
     9241 gcggccacgt tggcgcaacg catcggcgag cgcgacccat tcaccacgct gacgcagcag
     9301 cgcgatcagc ggatcgagcg cggccaggga gtccggctcg agctgaagtg cggtccggta
     9361 cgtcgagatg gcgtcctcgt tctggccgag ccgctcgcgc aggaccttgc cgaggaacac
     9421 gagcagaccg acctgcacgt cgtcggggcg ctccttcgca tccttggcgt gcacgatcga
     9481 atcgacgcgc gacgcggtct cccgccactg gccaagccga tccgagaggc gctcggtttc
     9541 ggacagcgcc tgcgtcgacg ccggatcgac cgcgagccag cggagcgctg catcgagcgc
     9601 acgcgcgcga tcgttcagct tgccttcagc gagctcgacg agccgctgca ggatgctcgc
     9661 cttgtcgagc ggatcgtcga cgacattgag cttggcttca aagatcgtca cgaggcgcgt
     9721 cgcgtctccg tcgttctcga gcagcggctc gaggatctcg acgatctctg acgacaggtc
     9781 ggcatgcttc gacagcaacc gctcgagcgc agcgcgcgat tgcgcgtggt ccggcgtgag
     9841 ctcgagcacc tcgcgatacg ctgcgaccgc ctgcttcgca gagccgagcg tcgtctcctg
     9901 cagctcgccg aggcggtaca ggtagtccgc cttcgtcaga tcgtcgtcgg agacgtcggc
     9961 ctggcgacgc agcacgtgcg cgagatccgg ccaccgcgac gcacgcgaca gcacgcgttc
    10021 gagcgcaccg agcgctgcct tcggctcggg gccttgcgac gcacgaccaa acgcgagcga
    10081 cgcgcggtcc atgtcgttga gcttgtcctc gcagatgtca ccgaggcgca tcgcatacgc
    10141 ttgctcgaca tccggcggca gcgacggcgc gtcgtcggcg aggcgctctt cgagcaggcc
    10201 cgccatctcg cgccacagac cctgcgattc ggcgaggcga agtagcggct cgagcaaatc
    10261 ggcattcgcc ggatcgtcgg cgagcgcgcg tccccacacg acgaacgcct gcgtcggttg
    10321 gttcgcaatc tgctcgcgca cctccgcgag cgccgtccaa tcggcgagtc gcgcagatgg
    10381 gtctctgccc tgcaccgcca agcggcgctc gtacacgcga accaggcccg cggcgtcgcg
    10441 ctccccgcga taaaccctct cgaggatcgg cgtgacggcc tcgacatggt cgtctttcgc
    10501 catcaaattc tcgagcgcag tgcgcgcctc ggcatgcgcg ggcatcacct gcagcacggc
    10561 gccgtatcgc gggatcgcgg cgtctggatc tgtcagctgc acttcgacga tgtgtgcgga
    10621 gcgatatgcg tagtccgcgc gatcttgctg cgacgtcgcg agcagcgctc gcttgtcgac
    10681 gatctcgagc agctcgggcc acatcttctg cttcgcgtag atgcgatcga gctcgccgag
    10741 cgcgcgcaaa tctcccgcat cgtcgtcgag cacagagctg agctgcccga tcgcttgata
    10801 gacgtcgtcg aggtggtgct cgtagacttg cgccgccgcg aggcggagct cctgccgcag
    10861 cgggaggtcc gtggtcagct cgatcttgcg ttcgagggtg acgaccatct cgcgcgcgtc
    10921 gccggtgtcg cgatagagcc gctcgagggc gtcgaggccc gcgaggtcac tgtcgtcgac
    10981 agcgagcacg ttcttgtatg cggcgatcgc gcgcggcttg tcctcgagga cctcttcgta
    11041 cagcgcggcg acgcggtgga gaagcacgag gcgaacgcct gcgtcgtccg tcacctccga
    11101 gcgacgctcg acgactttga cgagctcctc gacgcgacct tcgagcgcga gcaaccgatc
    11161 gagcgcatgg agcgctaccg gatcgtcacc gcggttctcg tcgaccttgc gccaggcttc
    11221 gatcgcacgc ttgagatccg cgcgctgctt ctcgtgaatc tcggcagact tcatccacag
    11281 cgacgccgcg agctcaccat ccgtcgacga cgcaccatct tcgatcgtgc gcgccgcttc
    11341 gtcccatgcg ccgagcttgc cggcgagcga gagcagcttc gtgagcgcga cgtcgtcggc
    11401 gacatcgatg cgccacgccc gcgccaacgc cgcaagtgcg agatcgatcg cgccgccgcg
    11461 ttgttcgtgc agctccgcga tctcgtgcag cgtgagcacc tgtgtcgccg ggtcgttcac
    11521 gtactcgagc ttcgcgtcga ggatgacgac gagcttctgc caccaatcct gctcgcgata
    11581 cacgggctcg agcagcgatg cgatgcgctc gcgatgctcg tcgtgaatca cgagccgctc
    11641 gagtgaattg accgcacggt cccacagcaa ccggccgctg atgacctcgt cgtattgatc
    11701 gatcgcggcc gtgatgtcgt tcaggtgctg ctcgtagact tctgcgagct tcagccgcag
    11761 cgcggcgact tcttcgaagt tgctcgcatc gctcgcgcgc gcttgctgcg cctcgaacag
    11821 atcgacgagg tcgcgccatt gaccgcgtgt gcggaacaag cgctcgagct cgccaactgc
    11881 ctcgcggtag ccctgctcga gaatcggcga ctcgccgctc tcggtgacgt tgacgacctc
    11941 gcgccaacct tcgatcgctt ttgccgcgtc accgatgccc gtctcgagca accgcgcgcg
    12001 cttcacgagt gcttcccggc gcagatcctc gtcggtgcgt gccgcaacgt cgtcgtagat
    12061 ctggaggagc tcgggccact tctgcgagcg cgcgagcatg ttctcgagcc ggctgacgag
    12121 atcgcgagcc gacgccttct gccgggctgt ctcttcctcg ggctcgatcg cgagcgcacg
    12181 gcgatacgcc gagtacgcgg catcgacgtt attgacgcgg cgctcgtaga tcacgccgag
    12241 cgcgatcgcg acgtcgcgga catcctcgtg ctcgctgctc tcgtcgtcga ggtactgctg
    12301 gtacgtctgt gcgacgaagt cccagttctc gacgacgcct gcgagacgat gcagctgctc
    12361 gcgcacgttc ggatcgcccg gcgactcgcg gaacagcttc gcgtaccact tcagcgcatt
    12421 ctcgaagtct tcgagctgct cttcgtagag ccgcgcgacg tagcgcgtga gcttgaggcg
    12481 ctcggccgga tcctgtgcgc cgtcgagctt gatcgaatag atgcggacga gatcgggcca
    12541 gcgctgctgc gccacgaaga tcggttcgag cacctcggcg gccatgaccc gcgcgtctgg
    12601 atcattgagg tactgctcga gcgcagcgag cgctaatggc tgacgcgcgt ggcccgacag
    12661 cgccgccgag tagttctcga tcgcagcttc gacgtcgtgc agctgcttgt cgtggatatc
    12721 gccgagctgc acgcgcagcg cgacggcttc ctcgaccgat gtcacgaagc caagacgacg
    12781 ctcgtacaga tcgacaacgt cgtgccatcg accttgttcg ctgtagagac cctcgagcgc
    12841 gtagatcgca tcggcgcgct ccggcgcgat ctggagcact tgctcccacg tcgagatcgc
    12901 gtcgtctggc cgcttgagct ggacgtagag gcttgcagcc tcgacgagct cgccgacctt
    12961 gtcgtcgacg tcgggcgcga tatccgcctg gcgcagcagg atatcgacga ggcgcgtatc
    13021 gtcttgcgtc tcgcggtaga tgctctcgag cgctgcgagt gaacggcggt cctcgggtgt
    13081 cgcgtcgagc accttttggt agtactcgcg cgcgagctcg ttatcgcgac gcaccgcgcg
    13141 tgcgaggtcg gcgatatcga gatagaggcg acgctggatt tcggcgtcga gcacgtcggg
    13201 cgcgacgtcg acgtagacgt cgatcagatc agcttcgcgt ccgagctcgc cggcgaggcg
    13261 ctcggtgtct gcgatgacgc tctggagctc cggctccgtc gccgcgtagc gcagcgcgag
    13321 cagctgaacc tcgaaggccc cgggcttgtc gccgaggcgg tattcgcgga gccgctcgac
    13381 ctcgaggagc gcgcgaagct tcgtcgccgg atcgtcggtc cactcggcct gcaacagcga
    13441 gagctgcgcg agccgctgct cctgaccggt tgccgcatac acgggccgca ggatgtcggc
    13501 cgcagcgacg cgcagatcga gatcgtcgag cgccttgttc acggcctcga gcgcgatcgg
    13561 atgctgcggg tcagagcgca gcacgcccgc ccatcgctcg agcgcttcga cgggacgcga
    13621 gagcgggccg ccgagcagcc gcgcgatcga gatctgcaga tcggtatgcg tcggcccgtc
    13681 gtagagcatc gcctgacgct cgtaaacatc gagcaggtcg gcccaccgct cggctccggc
    13741 gtagagacgc gcgagctcgt tgagcgcacg cgtgtcctcg gtatcgcggt cgacgatctc
    13801 gagccacgcc gcgatcgcct cgtcgacttc ctgcagcttc tgctcgtgca gctgcgcgat
    13861 gcgcgcgagc gtctcgatcg cctcgggctg cgtggtggcc atctcgacct tgcggcgcag
    13921 aacgtcgacg aggtctcgcc agttgcccgt gctctcgtag agccgctcga gcgcatgcag
    13981 cgcccccgga tcgttggcct gttcgccgag cacgtcgcgc cacgtggcga tcgatgcgtc
    14041 gcggtcgccg agcttctcct cgtcgagctg agcgactcgc gagagcagcg cacgtcgctc
    14101 gcctggatcc tccgcccact cggcctgctg ccgcgtgatc gcgcgcagct ctttccagtt
    14161 ctggctctct tcgtagaggc gcgcgagcgc ggagccggcg cgacgaatcg tctgcgggct
    14221 cgacggatcg gcttcgagta gtcgcttgta cgcggtaatc gcgcgctcgg tgtcgccgac
    14281 ctgcgtgtcg tagtacgtgg cgagctccgc gagcagtgct gcgcgcgttg ctggatcggt
    14341 atcgggcgtc gcggcgacgg ccgcttcgag cgcggcggtt gcttcgggcc agcggccgag
    14401 atactgcgcg agccgcgcta gctcgacgcg agcacgctcg tgcgccggct cgagcttcag
    14461 cacttcgagc cagcactcga acgcggtgcg tgcaccgttg agctcggcct cttcgatcgt
    14521 cgcgatgcgc gacagcagct cgaccgcctc ggtgcccgtc accatcggac gttgtgcacg
    14581 caacagggcg acgaggtcgc tccacagccg atcctgctcg tacagcggct cgagcagacg
    14641 gacgatgcgc atcatcacga tctgcgcgtt cggcgccgtc atcaattctt cgaggagatc
    14701 acgcgcaccg gcatgcgcac gggacttcgc gagcacgtcc tcgagcaaat cgaccgcacg
    14761 cgtcggatcg ccgagcttgt gcgcgaacag ctgcgcgcgg cggaacgcga tctcgacagc
    14821 gcgctcgcta tcgacatgat ccgtctgccg cgcgagcagc tcttcgagct ccttccagtt
    14881 gccgttcgac gagaacagcc gatcgagcgc gacgtacgcc ggcgcatgcg aggcatcgat
    14941 ctcgagcacg cggcgatacg cagcccctgc cctgcccgcg tcaccgagcg tctcctcttc
    15001 gagctgcgcg agctggatca gcgtcgacag gcgcgtctcc tgatcgatcg tgacctcggc
    15061 tcgccgctcg agcatcgcgc gcaggtcctg ccagcgttcg tcgtgacggt acatgcccgt
    15121 cagcatctcg aacgcgtcgg gcgcatccgg ctcgacctcg agcaccgtaa tccacgcgcg
    15181 ctcggcggtc ggccgatcac cgagccgctc gcccgcaatc ttcgcgagct ccgtcgcgac
    15241 tgcacggatc tgtcccgacg tgccgccctt cgacttgagc gtggtcagcg cacccgcgag
    15301 ctcgcctgcc cattcgccgt cgcggccgag ctggcctgac atcgcagcga gctggccgcg
    15361 gacgtcctga tccgctggat ccgctttgag cacgcgcaga cctgcagtcc acgccgccgc
    15421 gtcgtcgccc agatcgatcg cgtagagacg catcagcgtg cgatcgatgt cggtcttctc
    15481 acccgcaccg gtgtcatccg acgtgcgaat cacctcgagc gcagctgcct gctttgcggg
    15541 ctgattcgtc tgctcgagga acggcagcac ctgacgcgcg gctgcgagcc gatccttcgg
    15601 ctcgatcgcc gacgcgctcg tcggattcaa aatgatccgc gccactgcct cgatcgcctc
    15661 gggccttgca ccgccgccgg gcgcaggaac gccgagcgca tcgcggtagt aatcgagcgc
    15721 gttcgcgggc gcg
//
LOCUS       DATLBZ010000012         4240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8202750, whole genome shotgun sequence.
ACCESSION   DATLBZ010000012 DATLBZ010000000
VERSION     DATLBZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8202750"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VMZ53_00390"
     CDS             <1..781
                     /locus_tag="VMZ53_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUS26924.1"
                     /translation="PGRARQSLEQSSFDAWIKLYKPDESNLNTTVSYYLKGGLVMFAL
                     DLHIRRRTEGARSLDDVLRLMWQRFGATGEPHPDELQPIFEEATGIGLGDVFARQIRG
                     TEDPDLREELAHVGLELRMSMDPAASALEGGQGNWLGVTASANKVTGVFDGSPAQKAA
                     ITPGDELIAIDGFRTTNDTDLRSLIANRRPGETVAISLFRRHRLMELPCELAPSPPTR
                     YEIATVADAGPAAARYQAWLGEPHPGASQILATITTTARWV"
     gene            801..1556
                     /locus_tag="VMZ53_00395"
     CDS             801..1556
                     /locus_tag="VMZ53_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26925.1"
                     /translation="MGRFSLVLVALASLAACDKGKGESKGLPPAKDWNADVNEMNPVQ
                     QQGTAGTHSGGNPHADMGGGANPHAGIDMNNPHAGVDMGGAGGDPHAGVDMGGADPHA
                     GLDMGGGGNPHGGGVDVAKMGLPAPDPNRKIDPTHTVTGVIRVHAKAKSRLAAGGAIF
                     LVVKRVDATGAPTGTPLAVDKLTWANDEIPFEVSEKNAMVGGTELTGNVLVTARYDQD
                     GDALSKEPGDVVGTARVAIPANKVVITLDDVIN"
     gene            complement(1587..2423)
                     /locus_tag="VMZ53_00400"
     CDS             complement(1587..2423)
                     /locus_tag="VMZ53_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26926.1"
                     /translation="MRALATFALCLVPGVALAQSPKDKQKAGELVKQAIAKSQAGEHQ
                     AAIDLYNDAYKIIPQPLLLSNIGSEYQQMEHKEVEALGYFCKYLGSEGPNGQNSAYVT
                     AQAKLLYIKLGGVTDVKDEDVCKPIVKPTPVTPPEVTSTNPTNTTPVDTTPAGPVDSG
                     PKTKTPAMRYVGMGVAVVGAGMFGFGIYEGMQAKSISDEITNHPMNDPWDSNIKDREA
                     EGEAAEKKQIIFMAVGGVAVAAGVAMVFVFKPKTESAEQHVMVTPVVTGDTLGFAAAG
                     RF"
     gene            complement(2503..3363)
                     /locus_tag="VMZ53_00405"
     CDS             complement(2503..3363)
                     /locus_tag="VMZ53_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HUS26927.1"
                     /translation="MAVEAIPGADGELGVELRAAMALARAAGEVVLKYRGGELGVELK
                     AGDEPVTLADKAASELIVEGLAKQFSDPIVSEELELAEGVLQSSQAGRLWLIDPIDGT
                     KDFIRGSEGFSVMIGLLRAGRPVLGVVHQPAIDPPYGRTFIATPDGGSHVITTVGIYD
                     IHVSTVANAADARLVASASHRTVDIDKVKTVLGIGDEQNVGSIGVKLCLIALGLRDLY
                     VNPVAKTKVWDTCAPEAILAGAGGRISDLNGAQIDYTELRQRRGLVASNGVIHDEVVA
                     KLGPLFKKKP"
     gene            complement(3367..4227)
                     /locus_tag="VMZ53_00410"
     CDS             complement(3367..4227)
                     /locus_tag="VMZ53_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HUS26928.1"
                     /translation="MSTDKVIISCALTGAVTTKKHCPAIPYTPVEIAEEAKRAYDAGA
                     AIVHIHARNDDGSPTWNNATFQKIMDETKARCPVLINWSTGGAGPMSERITHLSQRPA
                     IAALNMGSMNYAKWSAEKKAFAFNFVFTNSFDDIVAFAKAMKEHNVKPEMECFDIGHT
                     NSHRVLADLGVISAPYHFSFIMGVLGGIPASARHLAFQAEQVPAGSRWKVIGISRDQW
                     QLAMAALSLGGDVRVGLEDNFYLPDGTMAKSNGDLVAAAVQLAKLSGRTVATVEETKQ
                     LLWPSGGGNF"
BASE COUNT          728 a         1382 c         1366 g          764 t
ORIGIN      
        1 gccaggtcgc gcgcggcaga gcctcgaaca gtcctcgttc gatgcgtgga tcaagctcta
       61 caagcccgac gagtcgaacc tcaacacgac ggtcagctac tacctcaagg gtggcctcgt
      121 gatgttcgcg ctcgatctgc acatccgtcg ccgcaccgag ggcgcacgct cgctcgacga
      181 cgtgctgcgc ctcatgtggc agcgattcgg cgcgaccggt gagccacacc ccgacgagct
      241 ccagccgatc ttcgaagagg cgacaggcat cggcctcggc gacgtcttcg cgcgccagat
      301 tcggggcacc gaggatccgg atcttcgtga agagctcgcg cacgtcggtc tcgagctgcg
      361 catgtcgatg gacccggccg catctgcgct cgaaggcggg cagggcaact ggctcggcgt
      421 caccgcgtct gcaaacaaag ttacaggcgt gttcgacggc tcacctgcgc aaaaagcggc
      481 catcacgccg ggcgacgagc tgatcgcgat cgacggcttc cgcacgacga acgacaccga
      541 tctgcgcagc ttgatcgcga atcgccgccc cggtgaaacc gttgcgatct cgctgttccg
      601 ccgtcaccgc ttgatggagc tgccatgcga gctcgctccc tcgccgccga cgcgttacga
      661 gatcgcgacc gtcgcggatg cgggtcccgc ggctgcgcgc taccaggcgt ggctgggtga
      721 gccccaccca ggcgcatcgc agattcttgc aacaatcacg acaacggcgc gctgggtata
      781 gcgctgctag actcgtcgcc atgggtcggt tctctctcgt cctcgtcgcg ctcgcatctc
      841 tcgccgcctg tgacaaaggc aaaggcgaat cgaagggcct tccgcccgcc aaagattgga
      901 acgcggacgt caacgagatg aatccggtgc agcagcaagg caccgcggga acacacagcg
      961 gcggcaatcc gcatgcagac atgggcggcg gcgcgaaccc gcacgcgggc atcgacatga
     1021 acaacccgca cgctggtgtc gatatgggtg gcgcgggcgg cgatccgcat gccggcgtcg
     1081 atatgggcgg cgccgatccg catgccggtc tcgacatggg cggcggcggt aatccgcacg
     1141 gcggtggcgt cgatgtcgcg aagatgggac tacccgcgcc cgatccaaat cgcaagatcg
     1201 atccgacgca tacggtgacc ggcgtcattc gcgtgcacgc aaaagcgaag tcccgcctcg
     1261 ccgcgggtgg cgcgatcttc ctcgtcgtca aacgcgtcga cgcgacgggc gctccgacgg
     1321 gcacgccgct cgcggtcgac aagctcacgt gggcgaacga cgagattccg ttcgaggtca
     1381 gcgagaagaa cgcgatggtc ggcggtaccg agctgacggg caacgtgctc gtcaccgctc
     1441 gctacgatca agacggcgac gcgctctcga aagaacccgg cgatgtcgtc gggacggcgc
     1501 gcgtggcgat tcccgccaac aaagtcgtga tcacgctcga cgacgtgatc aattaacggc
     1561 tccaaaacgg agcggtgggt gcggacctaa aagcggccgg ccgctgcgaa cccgagcgtg
     1621 tcacccgtga cgaccggcgt gaccatcacg tgctgctcgg cggattccgt cttcggcttg
     1681 aatacgaaca ccatcgctac accggccgcg acggcgacgc cgccgaccgc catgaagatg
     1741 atctgcttct tctcggcagc ctcaccctcg gcttcgcgat ccttgatgtt cgagtcccac
     1801 ggatcattca tcgggtgatt cgtgatctcg tcgctgatgc tcttggcttg catgccttcg
     1861 tagatgccga agccgaacat gcccgcgccg accaccgcga cgcccatgcc gacgtagcgc
     1921 atcgccggcg tcttcgtctt gggaccgcta tcgacggggc ccgctggcgt ggtgtcgacc
     1981 ggcgtcgtgt tcgtcggatt cgtgctcgtg acctcgggcg gcgtgacggg cgtcggcttc
     2041 acgatcggct tgcagacgtc ttcgtctttg acgtcggtga caccaccgag cttgatgtag
     2101 aggagcttgg cctgcgcggt gacgtacgcg ctgttctggc cgttcgggcc ttccgacccg
     2161 aggtacttac agaagtagcc gagcgcctcg acttctttgt gctccatctg ctggtactcg
     2221 gacccgatgt tcgagaggag cagaggctgc gggatgatct tgtaggcgtc gttatagagg
     2281 tcgatcgcgg cttggtgctc accggcctgg ctcttcgcga tggcctgctt cacgagctcg
     2341 ccggcttttt gcttgtcctt ggggctctgc gcgagcgcga caccggggac aagacacaac
     2401 gcgaacgtcg caagcgccct catggatcca atcgtatcac gtgaagatcg tcggtgcgcg
     2461 aaacgttctc tcgacggaaa tcacggccaa atcgtgacga ccttacggct tcttcttgaa
     2521 cagcggaccg agcttcgcga cgacctcgtc atggatgacg ccattcgacg cgacgagacc
     2581 gcgacgctgc cgcagctccg tgtagtcgat ctgggcgccg ttgagatcag agatgcggcc
     2641 gcccgcgccg gcgaggatcg cctcgggtgc gcaggtgtcc cagactttcg tcttcgcgac
     2701 tggattcacg tagaggtcgc gcaggccgag cgcgatcagg cacagcttca cgccgatcga
     2761 gcctacgttc tgctcgtcgc cgataccgag gaccgtcttt accttgtcga tatcgaccgt
     2821 gcgatgcgag gcactcgcga cgaggcgcgc gtcggcggcg tttgcgaccg tcgagacgtg
     2881 aatgtcgtag atgcccaccg tcgtgatgac atgcgagccg ccatcgggcg tggcgatgaa
     2941 ggtgcgaccg tacggcggat cgatcgccgg ttgatgcacg acgccgagca cgggacgccc
     3001 tgcgcgcaga aggccgatca tcacggagaa gccttcgctg ccgcggatga agtccttcgt
     3061 gccgtcgatc ggatcgatca gccacaggcg tccagcttgc gacgattgca gcacgccctc
     3121 ggcgagctcg agctcctcgc tgacgatcgg atcggagaat tgcttcgcca ggccctcgac
     3181 gatcagctcg ctggctgcct tgtcggcgag cgttaccggc tcgtcacccg ctttgagctc
     3241 cacgccgagc tcgccgccgc gatacttcag caccacctcg ccggcggcgc gcgcgagagc
     3301 catggcagcg cgcagctcga cgccgagctc gccatccgcg ccgggaattg cttcgactgc
     3361 caagagctag aagttaccgc cgccgctcgg ccagagcagc tgctttgtct cttcgacggt
     3421 ggcgacggtg cggcccgaca gcttcgcgag ctggaccgct gcagcgacga gatcgccgtt
     3481 gctcttcgcc atcgtgccgt cgggaagata gaagttgtcc tcgaggccca cgcggacgtc
     3541 gccgccgagc gagagcgccg ccatcgcgag ctgccattga tcgcggctga tgccgatgac
     3601 tttccagcgc gagcccgcgg ggacttgctc cgcttggaac gcgagatggc gcgcgctcgc
     3661 cggaattccg ccgaggacgc ccatgatgaa cgagaagtga tacggcgcgc tgatcacgcc
     3721 gaggtccgcg agcacgcgat gcgagttggt gtgcccgata tcgaagcact ccatctcggg
     3781 tttgacgttg tgctccttca tcgccttcgc gaatgcgacg atgtcgtcga acgagttcgt
     3841 gaacacgaag ttgaacgcga acgctttctt ctcggcgctc cacttcgcgt agttcatcga
     3901 gcccatgttg agcgccgcga tcgccggacg ctgcgagaga tgcgtgatgc gctcgctcat
     3961 cggacccgcg ccgccggtcg accagttgat caagacgggg cagcgcgcct tcgtctcgtc
     4021 catgattttc tggaacgtcg cgttgttcca cgtcggcgag ccatcgtcgt tgcgcgcgtg
     4081 gatgtggacg atcgcggcac ctgcgtcgta cgcgcgcttc gcctcttcgg cgatctcgac
     4141 gggcgtgtac ggaatcgccg ggcagtgctt cttcgtcgtc acggcgccgg tcagcgcgca
     4201 ggagatgatg accttgtcgg tggacacgag cggagcctac
//
LOCUS       DATLBZ010000013         6869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2245979, whole genome shotgun sequence.
ACCESSION   DATLBZ010000013 DATLBZ010000000
VERSION     DATLBZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245979"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VMZ53_00415"
     CDS             complement(<1..264)
                     /locus_tag="VMZ53_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26929.1"
                     /translation="MRIALACLLASGCDALFGVTHISSTTDGSTDGVNADAMSCHASS
                     VTANAGHSCAVRDDHGVSCWGNNTQNEIGTITGSISCQASGTQF"
     gene            complement(278..2029)
                     /locus_tag="VMZ53_00420"
     CDS             complement(278..2029)
                     /locus_tag="VMZ53_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HUS26930.1"
                     /translation="MSNIASMDSGILRVSTDRGERTYLLGTRTQADASPPVIDWRMSP
                     LAEAFFRAGPGEPYELENGKEGRVIERWIVDGERQIGDEVIVEGDRKQARPRPAPTKI
                     PQPDRSALIILDPEQQRAVDLPADTSLVVDGEAGVGKTLVALYRIASLQRRAQEKSRR
                     FRALVLVPTEGLRRLVRLISDRLGIEKLEIAVVDDWLVARAHAAFPGLPKRLSEGASA
                     QVIALKRHPAVRVVLDDFINWRPPKDNDDKLPRSRARLLHLWGDRDRLQRVLDASGGV
                     LQERAIVAAQKHTRIQFETTTEKQNRHVDADRLVALDGRKLDAGTAMEDVHSFDAEDV
                     PVLFELMRRGALPEAQLTDYDHIVLDEAQLRSPLELAAIGDALARGGTVTLAGDHRQA
                     TDETAYFTSWGESKNELRVQRWEEITLAITYRSVPAIGDFARGISDGRASVPGEPPSD
                     PALWASACPGHLAQAAQLCWHLDALQTRDPWRQIAVIARTAEHARRLHTELSRGLDPS
                     LVLDGDFRFEPGVIVTTASAVSGLEFDCVVIPDLTPPFYPANNPELVRALYVAATRAR
                     DWLWLLTPDSWSPLVAA"
     gene            complement(2105..2656)
                     /locus_tag="VMZ53_00425"
     CDS             complement(2105..2656)
                     /locus_tag="VMZ53_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS26931.1"
                     /translation="METYDELIEEANLALFFEDYDDAVDLLTRAIDVADDAEPPSVWG
                     NLGYALDKLKLYDAAGEAHRQCVELYEETDGPDHPRTLHKLGNLAFSLIGAGKVDEAE
                     ALLRRAVAGFDAQLDREPAWHVQALSNLGYFLLRSRRDPAAALACFARAEALCDAHDV
                     TGRPRDTVRQNRAEAEAAQIAGR"
     gene            complement(2668..4170)
                     /locus_tag="VMZ53_00430"
     CDS             complement(2668..4170)
                     /locus_tag="VMZ53_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HUS26932.1"
                     /translation="MKRIAFARIMQESNSLSPVATTLQDFEGSHYLEGDALMRAATDG
                     PEVAGFFKRAELAGFIQAARARKSEIEPVPLLSAWASSGGRLSQECFEELEARLVEQL
                     RRANVDGLYLSLHGAMGVHGISDPETRLLKSVRSVIGGAPLVVSHDLHGSLTQARVDA
                     CDALVAYQTNPHRDHAKIGKKAGAILIGTALGELKPTMAWRSLPMILGGGKTIDFLQP
                     MRPVFRRMRQAEKLGDALGASTFMVHPWNDSPQLGWSTLVVTDNDPAGADRLAEELAE
                     MCWERRHEQPPKFPGASEAIATARAAKVRRKLGCVTMADASDVVTAGAPGDSTHLLRA
                     LIEEGTGLRSYAAVRDPQAIAQLWPRDIGDIVATTIGGTLDPASSPPLPVRGTIISKH
                     DRHGFGRTIVLAVDHVRIVITEGPSMVMRPSFYTEVGLHPWHADIVMVKNFFPFLMFF
                     LPYNRKTIFVRTRGATDFDAAFQLPFDGPMHPRDPVDDWRARDRLRRGLS"
     gene            complement(4167..5504)
                     /locus_tag="VMZ53_00435"
     CDS             complement(4167..5504)
                     /locus_tag="VMZ53_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HUS26933.1"
                     /translation="MNIPSKGRLPNDILAELRGKSEGDTKWKQGRVFSLVYKVPGPGG
                     DAHDELLQKAHALYASTNLLNPMAFSSLKKMEREIIEMAGGLFHHAGAVGTVTSGGTE
                     SILCAVAAYRDRARAKRPWILRPELVVPTTIHPAFDKAAHYFGVKLKKVPVGPDFRAD
                     VRAMAKAIGWQTIGICASAPQYAHGVVDPIEELGALAQKKGLPLHVDACVGGYILPWL
                     EKLGRAIPRWDFRVPGVTSISADLHKFAYAGKGASTLVWRSLDDMRYQIFVATDFPGG
                     IYASPTMIGTRPGGPIAAAWATLQSIGADGYLALAKQAADAADRFRTGLRAIPGITLL
                     GESHSTIVSYGCVGADAYALADRLEARGWTVDRQQRPASIHLTVTANHAAIVDEYLRD
                     VRESLAELRANPALAKSGSAPMYGMAAKMPVRRLVARNVRKMIADLYASGAAS"
     gene            complement(5501..6802)
                     /locus_tag="VMZ53_00440"
     CDS             complement(5501..6802)
                     /locus_tag="VMZ53_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HUS26934.1"
                     /translation="MATLRTALAVLIALATSAHAGGFGIPEIGVRRTAMGAEIGRPDE
                     LSALYHNPAGLVLEDRWRLYISTGVSLLGTEFELQPWDRSDEFLNTMPESNGYYAPVK
                     PSRAMGVIPMIAAGGRIRDRLYVGGALYVGNATGAAFDEGAVTHYHLIDGYVIAPQLV
                     ASVAYQVTPDLAVAGTAGVINMRVHGKRYVFPIIDGNDISMLAGTTPELVLDGEAWAP
                     TWSVSAYGQPHPRVTWGAMVVGKVTAELEGPVTITYSDDASSPGDKLEGQQTTSQLLP
                     WTFQAGVNVDVTPNIEVGTEFRYWLYRQYKRQHTDIVGIFLVRELNTEKNYTDSWEST
                     GGVRVHDLAAAKGLDLMLGGQYDKSPAPAKTVTLDQPSFSHWGLHSGARYTVGRYRLG
                     ASYIHYWYRIPTITDSITLPPSNVRGHGANNIFTLSLEATL"
BASE COUNT         1119 a         2276 c         2317 g         1157 t
ORIGIN      
        1 gaactgcgta ccgctcgcct gacaactgat cgaccccgta atggttccga tctcgttctg
       61 cgtgttgttg ccccagcacg agacgccgtg gtcatcgcgc accgcgcagc tgtggcctgc
      121 gttcgcggtg acgctcgacg cgtgacaaga catcgcatcc gcgttgacgc catccgtcga
      181 cccgtcggtc gtcgacgaaa tatgcgtgac gccgaacaac gcgtcgcagc ctgatgcgag
      241 gaggcaggcc agcgctattc gcatccgtgc gatcgtacta cgcggccacg agcggcgacc
      301 atgagtcggg cgtgagcagc catagccagt cacgtgcacg ggtcgccgca acgtagaggg
      361 cgcgcacgag ctccgggttg tttgccgggt aaaacggcgg cgttagatcc ggaatgacga
      421 cgcagtcgaa ctcgaggccg gagacggccg atgctgtggt gacgatcacg cccggctcga
      481 accgaaagtc tccgtcgagg acgagcgacg gatcgaggcc gcgcgacagc tcggtgtgca
      541 gccgcctcgc gtgctctgcg gtgcgcgcga tgaccgcgat ctgtcgccac ggatcgcgcg
      601 tctgcagcgc gtcgaggtgc cagcatagct gcgctgcctg cgcgagatgc ccgggacacg
      661 cgctcgccca cagcgcagga tcggacggcg gctcgccagg cacgctcgcc cttccgtcgg
      721 aaatgccgcg cgcgaaatcg ccgatcgcag gcacggagcg atacgtgatc gcgagcgtga
      781 tctcttccca gcgctgcacg cgcagctcgt tcttcgactc tccccagctc gtgaagtacg
      841 ccgtctcgtc ggtcgcctgg cggtgatcgc cggcaagcgt cacggtgcca ccacgcgcaa
      901 gcgcgtcacc gatcgcggcg agctcgagtg gagagcgcag ctgcgcctcg tcgaggacga
      961 tgtggtcgta gtccgtcagc tgcgcttcgg gcagcgcgcc gcggcgcatc agctcgaaca
     1021 gtacgggcac atcctcggca tcgaagctgt ggacgtcttc catcgcggtg cccgcgtcga
     1081 gcttccgccc gtcgagcgct acgagccgat cggcatcgac gtgccgattc tgcttctcgg
     1141 tcgtcgtctc gaattggatc cgcgtgtgct tctgcgccgc gacgatcgcg cgctcctgca
     1201 gcacgccgcc cgatgcatcg agcacgcgct gcaaccgatc gcgatcgccc cacaggtgta
     1261 gcaaccgcgc gcgcgaccgc ggcagcttgt cgtcgttgtc cttcggcggc ctccagttga
     1321 tgaagtcgtc gagcacgacc cgcacggcgg gatgccgctt gagcgcgatg acctgcgcac
     1381 tggcaccctc gctgagccgc ttcggcaagc ccggaaacgc cgcgtgagcg cgcgcgacga
     1441 gccagtcgtc gacgaccgca atctcgagct tctcgatgcc gagtcgatcc gaaatcagcc
     1501 gcacgagccg tcgcaagccc tccgtcggca cgagcacgag cgcgcgaaac cgcctcgact
     1561 tttcctgcgc acggcgctgc agcgaagcga tccgatacaa cgcgacgagc gtctttccga
     1621 cgcccgcctc gccgtcgacg accaaggacg tatccgcagg taagtcaacc gcgcgctgct
     1681 gctcggggtc gaggatgatg agggcgctgc gatccggctg cgggatcttc gtcggtgcgg
     1741 gccgcggtcg ggcctgcttg cgatctccct cgacgatgac ctcgtcgccg atctggcgct
     1801 cgccatcgac gatccagcgc tcgataaccc tgccctcctt gccgttctcc agctcgtagg
     1861 gctcgcccgg accggcgcga aaaaatgcct cggccagcgg cgacatgcgc caatcgatca
     1921 cgggcggcga agcgtccgct tgcgtccgcg ttccgagcag atacgtccgc tcgccgcgat
     1981 ccgtgctcac gcgcaggatg ccgctgtcca tgctcgcgat attcgacatt ccgcgcgagc
     2041 tgacaagcta gaaagtcact cgcgaacagc gctatcatct ataggcggga tgcgcgcggc
     2101 gccttcagcg tccggcgatt tgcgcggcct ctgcctcggc gcgattctgc cgcacggtgt
     2161 ctcgtggcct gccggtgacg tcgtgcgcat cgcacagcgc ctcggcgcga gcgaagcacg
     2221 cgagcgcagc tgccgggtca cgacgcgacc gcagcaggaa gtagccgagg ttcgagagcg
     2281 cctgcacgtg ccacgcaggc tcgcgatcaa gttgtgcgtc gaagccggcg accgcgcgcc
     2341 gtagtagcgc ttctgcttcg tccaccttgc cggcaccgat cagcgagaac gcgaggttgc
     2401 cgagcttgtg cagcgtgcgt ggatggtcgg ggccgtcggt ctcttcgtac aactcaacgc
     2461 attggcgatg cgcttctcct gctgcgtcgt agagcttgag cttgtcgagg gcgtagccga
     2521 ggtttcccca gacggacggc ggctcggcgt cgtcagccac gtcgatggcg cgcgtgagga
     2581 gatcgaccgc gtcgtcgtag tcttcgaaga agagcgcgag gttcgcttct tcgatgagct
     2641 cgtcgtacgt ctccatgaaa gcacgtctta cgagaggccg cggcgcagtc gatcgcgcgc
     2701 acgccagtca tcgaccgggt cgcgcgggtg catcggcccg tcgaatggca gctggaacgc
     2761 ggcatcgaaa tcggtcgcgc cgcgcgtgcg cacgaagatc gtcttgcggt tgtacggcag
     2821 gaagaacatc aggaacggga agaagttctt caccatcacg atgtcggcgt gccacgggtg
     2881 gaggccgacc tccgtgtaga acgacggtcg catcaccatc gacggtcctt cggtgatcac
     2941 gatgcgcacg tggtcgacgg cgaggacgat cgtgcggccg aagccgtggc ggtcgtgctt
     3001 gctgatgatc gtgccgcgta cggggagcgg tggcgaggag gccgggtcga gcgtgccgcc
     3061 gatcgtcgtc gcgacgatgt cgccgatgtc gcggggccag agctgcgcga tcgcttgcgg
     3121 gtcgcgaacg gcggcgtacg agcggaggcc cgtgccttct tcgatgagcg cgcggagcag
     3181 gtgcgtcgag tcacccggcg cgccggcggt gacgacgtca gaggcgtcgg ccatggtcac
     3241 gcagccgagc ttgcggcgga ctttcgccgc gcgggcggtc gcgatggctt cgctcgcgcc
     3301 tgggaatttc ggcggttgct cgtgccggcg ctcccagcac atctcggcga gctcctcggc
     3361 gaggcgatcg gcgcccgcgg ggtcgttgtc cgtgacgacg agcgtcgacc agccgagctg
     3421 cgggctgtcg ttccatgggt ggaccatgaa cgtcgaggcg ccgagagcgt cgccgagttt
     3481 ttcggcttgg cgcatccgcc ggaagacagg gcgcatcggc tggaggaaat cgatcgtctt
     3541 gccaccgccg aggatcatcg ggagggagcg ccacgccatc gtcggcttta gctcgccgag
     3601 cgcagtgccg atgaggatcg cgcctgcctt cttgccgatc ttggcgtggt cgcggtgcgg
     3661 gttcgtttga tacgcgacga gcgcgtcaca cgcgtcgaca cgggcttgcg tgaggctgcc
     3721 gtgcagatcg tgcgagacga cgagcggcgc accgccgatc accgaacgca cgctcttcaa
     3781 gaggcgggtt tctggatcgg agatcccgtg cacgcccatc gcgccgtgga ggctaagata
     3841 gaggccgtcg acatttgcgc gccgcagctg ttcgacgagc cgcgcttcga gctcttcgaa
     3901 gcattcctgc gagaggcggc cacccgacga cgcccatgcg gagagcagcg gcaccggctc
     3961 gatctcggac ttgcgagcgc gtgctgcttg gatgaagccc gcaagctcgg cgcgcttgaa
     4021 gaagccggcg acctcgggac cgtcggtggc ggcgcgcatg agcgcatcgc cttcgaggta
     4081 gtggctgcct tcgaagtctt gcagcgtcgt cgcgaccggg gacagcgagt tcgactcctg
     4141 catgatgcgc gcgaacgcga tgcgcttcac gacgcggctc ccgatgcgta gaggtccgcg
     4201 atcatcttgc ggacgttgcg cgcgacgagc cggcgcaccg gcatcttcgc ggccatgccg
     4261 tacatcggcg cactgccgct cttcgcgaga gcggggtttg cgcgcagctc ggcgagcgat
     4321 tcgcgcacgt cgcgcaggta ctcgtcgacg atcgcggcgt ggttcgcggt gaccgtgaga
     4381 tggatcgacg cggggcgctg ctggcgatcg accgtccagc cacgtgcttc gagccgatcg
     4441 gcgagcgcgt acgcgtcggc acctacacag ccgtacgaca cgatcgtcga gtggctctcg
     4501 ccgaggagcg tgatgcccgg aatggcgcga aggccggtgc gaaagcgatc cgcggcatcg
     4561 gccgcttgct ttgcgagcgc gaggtaaccg tcggcgccga tcgattgcag cgttgcccac
     4621 gctgcggcga tgggcccgcc cggccgcgtg ccgatcatcg tcggcgatgc gtagatgccg
     4681 cccggaaagt ccgtggcgac gaagatctgg tagcgcatgt catcgagcga gcgccacacg
     4741 agcgtcgacg cgcctttacc cgcgtacgcg aacttgtgga ggtcagcgct gatcgacgtc
     4801 acgccgggaa cgcggaagtc ccaacgcgga atcgcgcgac cgagcttctc gagccacggc
     4861 aagatgtagc caccgacgca cgcatcgacg tgcagcggca agcccttctt ctgcgcgagc
     4921 gcgccgagct cttcgatcgg atcgaccacg ccgtgcgcgt attgcggtgc cgacgcgcag
     4981 atgccgatcg tctgccaacc aatcgccttg gccatcgcgc gaacatccgc gcggaagtcg
     5041 ggaccgacgg gcaccttctt caacttcacg ccgaagtagt gagccgcctt gtcgaacgcg
     5101 ggatggatcg tcgtcgggac gacaagctcg ggccgcagaa tccacggccg cttggcacgc
     5161 gcgcgatcgc gatacgcagc gacggcacag aggatcgact cggtcccccc cgatgtcacc
     5221 gtcccgaccg cgccggcgtg gtgaaacagg ccgcccgcca tctcgatgat ctcgcgctcc
     5281 atcttcttga gcgacgaaaa cgccatcggg ttgagcagat tcgtcgacgc gtagagcgcg
     5341 tgcgccttct gcagcagctc gtcgtgcgcg tcgccacccg ggcccggcac cttgtagacg
     5401 agcgagaaca ccctgccctg cttccacttc gtgtcgcctt cggacttgcc gcgcagctcg
     5461 gcgaggatgt cgttcggcaa gcggcctttg ctcgggatgt tcatagtgtc gcctccagcg
     5521 agagcgtgaa gatgttgttc gcgccgtgac cgcgcacgtt ggacggcggc agcgtgatcg
     5581 aatcggtgat cgtcggaatc cgataccagt agtggatgta gctcgcgccg agccgatagc
     5641 ggccgaccgt gtagcgcgcg cccgagtgca ggccccagtg cgagaacgat ggctgatcga
     5701 gcgtcacggt cttcgcgggc gcgggcgact tgtcgtattg cccgccgagc atcagatcga
     5761 ggcccttcgc cgcggcgagg tcatgcacgc gcacgccgcc tgtcgactcc cacgagtcgg
     5821 tgtagttctt ctccgtgttg agctcgcgca cgaggaagat gccgacgatg tcggtgtgct
     5881 gccgcttgta ctggcgatag agccaatagc gaaactcggt gccgacctcg atgttcggcg
     5941 tcacgtcgac attcacgccc gcttgaaacg tccacggcaa cagctgcgac gtcgtctgct
     6001 gaccttcgag cttgtcgccc ggcgacgatg cgtcgtcgga gtacgtgatc gtgaccgggc
     6061 cttcgagctc ggctgtcacc ttgccgacga ccatcgcgcc ccacgtcacg cgcggatgcg
     6121 gttgaccgta cgcggacacg ctccatgtcg gcgcccacgc ctcaccgtcg agcacgagct
     6181 cgggcgtcgt cccggcgagc atgctgatgt cgttgccatc gatgatcggg aacacgtacc
     6241 gcttgccgtg cacgcgcatg ttgatcacac ccgcggttcc ggcgaccgcg aggtccggtg
     6301 tcacctgata cgcgaccgac gcgacgagct gcggcgcgat cacgtagccg tcgatcaagt
     6361 gatagtgcgt gaccgcgcct tcatcgaagg cagcgcccgt cgcgttgccg acgtagagcg
     6421 cgccgccgac atacagacga tcgcgaatgc ggccgcccgc tgcgatcatc gggatgacgc
     6481 ccatcgcgcg gctcggcttg accggcgcgt agtagccgtt cgactcgggc atcgtgttca
     6541 gaaactcgtc gctgcggtcc cacggctgca gctcgaactc ggtcccgagc agcgagacgc
     6601 cagtcgagat gtagaggcgc cagcgatcct cgagcacgag tcccgcgggg ttgtgataga
     6661 gcgccgacag ctcgtcgggg cggccgatct ctgcacccat cgcggtgcgc cgcacgccga
     6721 tctcggggat gccaaaaccg cccgcatgcg cgcttgtcgc gagcgcgatc agaacagcga
     6781 gcgcggtgcg aagcgtggcc acgaaccctc agtaacccgc cggcccacaa gggtccacac
     6841 ggatcgaacg cacgtcagat tctattcgc
//
LOCUS       DATLBZ010000014         8450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10438884, whole genome shotgun sequence.
ACCESSION   DATLBZ010000014 DATLBZ010000000
VERSION     DATLBZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10438884"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VMZ53_00445"
     CDS             complement(<1..525)
                     /locus_tag="VMZ53_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HUS26935.1"
                     /translation="MARREIAQRNEAADTGEAQADRGDTTRATDSGFTSKGAIPPVES
                     GFASAKPSASERAFGAGHDEDLWAKGDELDAKDDAERRRDQRFDNDAVLEEIAAGTKV
                     VKTGDKGRAVTKLQVALLDLGYLKGGQVDGDFGGETETALKKFQAAASVGPTGEFDAA
                     TLDGLRTRFGNRKPY"
     gene            complement(580..1326)
                     /locus_tag="VMZ53_00450"
     CDS             complement(580..1326)
                     /locus_tag="VMZ53_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26936.1"
                     /translation="MPKRWLLPIVVGACVGPPPREPYVVAPDTSVVERVVVGASVEMA
                     TTAYGGCRVTTHVVGVDLGTSHSSKDWTGLGLKRESEVVGCHDQGVEAKVFCSPRDMC
                     EMATDEDQGTGAASIPVRLAKLGSVKATLEVNNLETGNHTTTVRRFEVIPPTQIRVQC
                     MTPALAWGSCDAGMSAAKPLLRVMLLLDGKPVRSSLLRVNGHAATSSSSFTIGQSLEP
                     ILGSSPIAPGTYELDVAVGDLHEHVSVAIQ"
     gene            complement(1319..2404)
                     /locus_tag="VMZ53_00455"
     CDS             complement(1319..2404)
                     /locus_tag="VMZ53_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS26937.1"
                     /translation="MLAPTIKKELLLLRRDPRVIVRLLFLPLAFIAIFGMVFNAQDKR
                     GSHARPLALWADPAVKETPRITLALEGSKQFVIVSCASPQEVTQRVLSGKVDAGIVLP
                     ADFDPAHGHPGTLVFDRDASPQVTAPLEGMLVGIVGRAYEGLGEPPTVLATTRAGTQA
                     AEPTSSFQVVVPGNSVLFGFLIALTCAMSFSEERRTGTWRRLLASPAPMWTLLVGKLL
                     PYIILGSLQVGLLLVVGALGFGMKVTGSIPALALLTISVACCATTLGLAIAAFSTTEK
                     QISSLGSVLILTMGLLGGCMVPRMLMPPLMQTIGLAVPHGWALDGYNKLIVGGGGFAD
                     VAKNVAAVFGFALLFGVIGVKRYRFHA"
     gene            complement(2404..3309)
                     /locus_tag="VMZ53_00460"
     CDS             complement(2404..3309)
                     /locus_tag="VMZ53_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS26938.1"
                     /translation="MAAVLELKGLGRDYGTRAAVKALDLTIEKGEIFGLLGPNGAGKT
                     TSISMACGVVAPSRGSVRVAGHDLATDGTAARRAIGLVPQELAIYEDLTPRQNLTYFG
                     ELYGAGAAAVDKALGIAELRDRADDLVKDLSGGMKRRLNLAAGLVHTPALLVCDEPTV
                     GVDPQSRRHIFDSIKKLRHEGMTILYTTHYMEEVEVLCDRIAIMDAGEVIACGTLAEL
                     QARHASTGVELELEGDDATLDAAARAAGVTRDGAVLRLDMRPEWTSVISAIEATGARI
                     TEMRSRSANLETLFLALTGRSLRDA"
     gene            3367..3771
                     /locus_tag="VMZ53_00465"
     CDS             3367..3771
                     /locus_tag="VMZ53_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26939.1"
                     /translation="MSTYVRYFAETPVTLKALSAAIKAAAPDYKIDGGDLMRGSDVLA
                     EVGIDSAGSDLFVEELNARLGAIGQMGGQAAQWVGARLQGTQSIVSIRVEPQNWDALA
                     PLWTALPSLASGLTQVDGQGYYDGSNLVVSIA"
     gene            complement(3775..5298)
                     /locus_tag="VMZ53_00470"
     CDS             complement(3775..5298)
                     /locus_tag="VMZ53_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HUS26940.1"
                     /translation="MKYRFVVATLLGVACGSSQQARQQSARELVASAHGTSVFGPIDE
                     WMPSPQPGVGRTGMFDVRPPDQCEVAVLPGGEDSFATRMHLLSLAKKTIRIQALIFTG
                     DESGLRVSEVLKQKKAAGVDVKVIVDSINNTSLQTQWMYFDLKQHGIEVEGYEALGLQ
                     LLNELPIPFVSSHQDPNKRYHEKVWIIDAGTPNAQAVTGGLNIANEYFRIDPSNVRRY
                     WRDQDVVVRGAIIDDLTTMFDRNYDHFKQIKASRGGLSDSAWAATRAIMAKTGTVEIS
                     NTQRDDLRANVAAFEARPPITSFKPARCRFLQSRPRLEETFILQAYLKLLDMAKREVL
                     IANAYFVPTPAIKMALKAAAARCVRVVLLTNAPETNDTPGMSLLGRGHYADLLSANSQ
                     AKSCPGLEIWEWQGRRANEPKQTQGLIHAKFALVDRTISLVGSYNLDPRSEFLNSESA
                     IVYENAELTEELAHTYLEQDLATARRITAEEAASFAKPDTTIDRFKTELARLFEDQM"
     gene            complement(5303..6382)
                     /locus_tag="VMZ53_00475"
     CDS             complement(5303..6382)
                     /locus_tag="VMZ53_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUS26941.1"
                     /translation="MGLAELFEELYRLKGSDVHIAPGYPPTLRVRGELLPLGDERLTM
                     EDADAMLMPLLQEHHRRVFDATGDLDWAYQYDTRGRFRVSYFRKTTGVGGVFRSIPSK
                     IATLDDLAVPQGVRKLVEVRSGLVLVTGPTGSGKTTTLAAMIDQINRTRPVRILTIED
                     PIEFIHKSRKAIVTHKEIGHHVPSFVDAIRSASRENPDVILVGELRDSETMKAALQLA
                     SFGILVFASVHTNSASATIDRYVNAFSADQQPQIRGLLAESLAGIIAQQLVRKADGVG
                     RVAVHEILFSSIALSGLIREAKPQQIPSLMQSGLADGMQPMDWAIERAVLSGAITARD
                     GLEKALDKDTFAKVPSIASRLANES"
     gene            complement(6382..7821)
                     /locus_tag="VMZ53_00480"
     CDS             complement(6382..7821)
                     /locus_tag="VMZ53_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUS26942.1"
                     /translation="MPRLTTDVYKPEPLRGQSAPDPGFSLEDDGIVADVPDLDLAYAT
                     ALGGGGEIVDDAEDASGAAYGTPEVAAAEVYAPEWYGPQIVDEHTDITSLHALLEAAY
                     RRSASDLHIIANQPITIRRLGQLTPLEAKALTEPFDRAVYSALFGPLDPVLAERFLLP
                     LLGPSSRQRLGEVGYVDLGFQVPGRGRIRANISQQQDGLAGSFRLCRDTIPTLAGLGL
                     PRELEKVIGHHQGLVVIAGPSGHGKTTTMAALVDLLNTSNSDHIITIEDPVEIEHPAK
                     RAIVSHREVGRHTLSFVSALKASLREDPDVIVIGELRDRETVEIALAAAETGHLVLST
                     ISTPSAVKTIDRLIDMFPPDDQVQVRASISGTLRAVIAQRLLPNATQDGMVAAIEMVT
                     GVLPLATMIRDNKMFQLPSLMQRGRAFGMIRLDDSLVEHVRAKRITEEVALGAAENKR
                     DLAALLGGRTTPSPTSSRGLFGGKGGPTS"
     gene            7808..>8450
                     /locus_tag="VMZ53_00485"
     CDS             7808..>8450
                     /locus_tag="VMZ53_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26943.1"
                     /translation="MSRGIVAVLSGVTVSGGVVPPPPRPVGGISGLPIVDVSVRGGAV
                     VVAVVRGAAVVVVAVRGAIVVPALRASLRDVSSGRGEAVVLAARGEMGAPVGDIIVAL
                     RPDVDDGGAAVEEAVRTEPTGASSIGSVGSGIVVRGSDAVVAAALVGDITVALRPVVD
                     DGGAAVEEVMRTDTPALSSIGAVGSGVIVRGADVGEVVRTETMGASSIGADGGG"
BASE COUNT         1378 a         2710 c         2848 g         1514 t
ORIGIN      
        1 gtagggtttg cgattgccga atcgcgtgcg cagaccgtca agagtggcgg cgtcgaattc
       61 gccggtcggg ccgacactag cggccgcttg gaacttcttc agcgcggtct cggtttcgcc
      121 gccgaagtcg ccgtcgacct gcccgccttt gaggtaaccg agatcgagta acgcgacttg
      181 gagcttcgtg accgcgcggc ccttgtcgcc cgtctttacg actttcgtgc ccgccgcgat
      241 ttcttcgagg acagcatcgt tgtcgaagcg ctgatcgcgg cgccgctcgg cgtcgtcttt
      301 ggcatcgagc tcgtcgcctt tggcccagag atcttcgtca tggccggcgc cgaatgcgcg
      361 ctcggacgcc gatggtttcg cgctcgcgaa gcccgactcg accggcggaa tggcgccctt
      421 cgaggtgaag ccgctgtcgg tcgcgcgggt cgtgtcgccg cggtcggctt gcgcctcgcc
      481 cgtgtcggca gcctcattcc gctgagcaat ctcgcgacgc gccataacgc tcctcctgca
      541 ccgatctacg tactgtacac gttagaactt cctcgggatt tactggatcg caacgctgac
      601 gtgctcgtgc aggtcaccga cagcgacgtc gagctcgtag gtccccggcg cgatgggact
      661 gctgccgagg atcggctcga gcgattgacc gatcgtgaag gacgaggacg acgtggccgc
      721 atggccgttg acgcgcaaga gcgacgaacg caccggcttg ccgtcgagga gcagcatcac
      781 gcggagcaac ggtttcgcag ctgacatgcc ggcgtcgcac gagccccacg ccagcgcagg
      841 cgtcatgcat tggacgcgga tctgggtcgg tgggatgacc tcgaagcggc gtacggtcgt
      901 cgtgtggttg cctgtctcga ggttgttgac ctcgagcgtg gctttgacgc tgccgagctt
      961 cgcgagccgc accgggatgc tcgcagcgcc ggttccttga tcctcgtccg tcgccatttc
     1021 gcacatgtcg cgcgggctgc agaacacttt cgcctcgacg ccttggtcgt gacagccgac
     1081 gacctcgctc tcgcgcttta ggccgagacc tgtccagtcc ttcgagctat gcgacgtgcc
     1141 gagatcgacg ccgacgacgt gtgtcgtcac gcggcagccg ccatacgctg tcgtcgccat
     1201 ctcgacggat gcgccgacga ccacccgctc gacaacggac gtatcaggcg cgacgacgta
     1261 cggctctctc ggcggcgggc cgacacacgc tccgacgacg atcgggagaa gccaccgttt
     1321 aggcatggaa ccgatatcgt ttcacaccga tcacaccgaa cagaagcgca aagccgaaaa
     1381 ccgctgcaac gttctttgcg acgtctgcga aaccgccgcc tccgacgatc agcttgttgt
     1441 agccatcgag cgcccagccg tgcggcacgg cgagaccgat cgtctgcatc agcgggggca
     1501 tcaacatgcg cggcaccatg cagccaccga gcaggcccat ggtcagaatg aggacggagc
     1561 caagcgacga aatctgcttc tcggtcgtgc tgaacgcggc gatcgcgagg ccgagcgtgg
     1621 tcgcgcagca ggcaacgctg atcgtcaaca gcgcgagcgc ggggatgctg ccggtcacct
     1681 tcatgccgaa gccgagcgcg cccacgacga gcaagaggcc gacctgcaac gagccgagga
     1741 tgatgtacgg caacagcttg ccgacgagca gcgtccacat cggcgccggc gatgcgagca
     1801 aacgacgcca cgtgcccgtg cgacgttctt cggagaacga catcgcgcat gtcagcgcga
     1861 tcaagaagcc gaacagaacg gagttgccgg ggaccacgac ctgaaacgac gacgtcggct
     1921 cggcggcttg cgtgcccgcg cgcgtcgttg cgagcaccgt cggtggctcg ccgagtccct
     1981 cgtatgcgcg tccgacgatt ccgacgagca tgccctcgag tggcgccgtc acttgcggcg
     2041 acgcgtctcg atcgaagacg agcgttccgg gatgcccgtg cgctggatcg aagtcggccg
     2101 gcagcacgat tcctgcgtcg accttgccgg agagcacgcg ttgcgtgacc tcttgcgggc
     2161 tcgcgcacga gacgatcacg aattgcttcg agccttcgag cgcgagcgtg atgcgcggtg
     2221 tttccttcac agcggggtcg gcccacagcg ccagcggacg tgcatgcgag ccgcgcttgt
     2281 cctgcgcgtt gaacaccatc ccgaagatcg cgatgaacgc gagcggcaag aagagcagcc
     2341 tgacgatgac gcgcggatcg cggcgcagca gcaacagctc cttcttgatc gtcggtgcca
     2401 gcatcacgcg tctcgcagac tgcgcccggt gagcgcgagg aagagcgtct cgagattcgc
     2461 gctgcgcgat cgcatctccg tgattcgcgc gccggttgcc tcgatggccg agatgacgct
     2521 ggtccactcc ggccgcatat cgaggcgcag caccgcacca tcgcgggtga cacctgccgc
     2581 gcgtgcagcc gcgtcgagcg tcgcatcgtc accctcgagc tcgagctcga cgcccgtcga
     2641 tgcgtggcgc gcctgcagct cggcgagcgt gccgcatgcg atcacctcgc ccgcgtccat
     2701 gatcgcgatc cgatcgcaga ggacctcgac ttcttccatg tagtgcgtcg tgtagaggat
     2761 cgtcatgcct tcgtggcgca gcttcttgat cgaatcgaag atgtggcggc ggctctgcgg
     2821 atcgacgccg accgtcggct cgtcacacac gagcagcgcc ggcgtatgca cgaggcccgc
     2881 cgcgagattg aggcgacgct tcatgccgcc gctcaagtcc ttcacgagat cgtcggcgcg
     2941 atcgcgcagc tctgcgatgc cgagcgcctt gtccaccgcc gccgctcctg cgccgtagag
     3001 ctcgccgaag tacgtgaggt tctgccgcgg agtcaggtcc tcgtagatcg cgagctcttg
     3061 cgggacgaga ccgatcgcac gtcgggcggc cgttccgtcg gtcgcgaggt cgtggccggc
     3121 gacgcggacg gagccacgcg atggcgcgac gacgccacat gccatcgaga tcgacgtcgt
     3181 cttgccggcg ccattcggac cgagcaagcc gaagatctcg cccttctcga tggtgagatc
     3241 gagagctttc acggcggcac gcgtgccgta gtcgcgcccc aatcccttca gctcgaggac
     3301 cgctgccacg ggcgtactct actccacccc agccccacct tttcgtggca gagtctgctc
     3361 gctgtcatga gcacctacgt ccgctacttc gccgagaccc cggtcacgtt gaaggcgctc
     3421 agcgccgcga tcaaagcggc tgcgcccgac tacaagatcg atggcggcga cttgatgcgt
     3481 ggctcggacg tgctcgccga agtcggaatc gacagcgcgg gcagcgacct gttcgtcgag
     3541 gagctcaacg ctcggctcgg cgccatcggc cagatgggcg gtcaagcggc gcagtgggtc
     3601 ggtgcgcgcc tgcagggtac gcaatcgatc gtgtcgattc gcgtcgagcc gcagaattgg
     3661 gatgcgctcg cgccactgtg gaccgcgctg ccgagcctcg cgtcgggcct cacgcaggtc
     3721 gatggccagg gctactacga cggcagcaac ttggtcgtct cgatcgcctg acgctcacat
     3781 ctgatcttcg aacaggcgcg cgagctcggt cttgaaccga tcgatcgtcg tgtcgggctt
     3841 tgcaaagctc gcggcttctt cggcggtgat gcggcgagcg gtggcgaggt cctgctcgag
     3901 gtacgtgtga gcaagctcct cggttagctc cgcattctcg tagacgatcg ctgactcgct
     3961 gttgaggaat tcactgcgtg gatcgaggtt gtaggagccg acgaggctga tggtccgatc
     4021 gacgagcgcg aacttcgcgt ggatgaggcc ctgcgtctgc ttcggctcgt ttgcgcgccg
     4081 gccttgccac tcccagatct cgaggcccgg gcagctcttc gcctgcgagt tggcgctgag
     4141 cagatccgcg tagtggccgc gaccgagcag tgacatgccc ggcgtatcgt tcgtctcggg
     4201 cgcgttcgtg aggagtacga ctcgcacgca acgcgcggcg gcggccttga gtgccatctt
     4261 gatcgctggc gtcgggacga agtacgcgtt tgcgatcagc acttcacgct tcgccatgtc
     4321 gaggagcttg agatacgcct ggaggatgaa cgtctcctcg aggcgcggcc gactctggag
     4381 gaagcgacag cgtgcgggct tgaagctcgt gatcggcggg cgcgcctcga aggcagcgac
     4441 gttcgcgcga aggtcgtcgc gctgcgtgtt cgagatttcg acggtgcctg tcttcgccat
     4501 gatcgcgcgc gtcgcggccc acgccgagtc ggaaagcccg ccgcgtgacg ctttgatctg
     4561 cttgaagtga tcgtagttgc ggtcgaacat cgtcgtgagg tcgtcgatga tcgcgccgcg
     4621 gacgaccacg tcttgatcgc gccagtagcg acgcacgttg ctcggatcga tacgaaagta
     4681 ctcgttcgcg atgttgaggc cgcccgtcac cgcttgggcg ttcggcgtgc ctgcgtcgat
     4741 gatccagacc ttctcgtgat agcgcttgtt tggatcttgg tgcgacgaga cgaacgggat
     4801 cggtagctcg ttgagcaact gcagaccgag tgcctcgtag ccttcgacct cgatgccgtg
     4861 ttgcttgaga tcgaagtaca tccactgcgt ctgcagggat gtgttgttga tgctgtcgac
     4921 gatcaccttc acgtcgacgc ctgctgcctt cttctgcttc agcacttcgc tcacgcgcag
     4981 gccggattcg tctcccgtga agatcagcgc ctggatgcgg atggtcttct tcgcgaggga
     5041 gagcaaatgc atccgcgtcg cgaacgaatc ctcgccgcca ggcagcacgg cgacctcgca
     5101 ctggtcgggc gggcgcacgt cgaacatgcc ggtgcgtcca acgccgggct gtgggctcgg
     5161 catccactcg tcgatcggac cgaagacgga cgtgccgtga gcggaagcga ccagctcacg
     5221 agcggattgt tggcgtgcct gctgtgacga gccacacgcg acgccgagca gcgtggcgac
     5281 gacgaatctg tacttcatgg gatcaggact cgttggcgag gcgcgatgca atcgatggga
     5341 ccttggcgaa ggtatccttg tcgagcgcct tttcgaggcc gtcgcgcgca gtgatcgcgc
     5401 ccgagagcac ggcccgctcg atcgcccagt ccatgggctg catgccgtcg gcgaggcccg
     5461 attgcatgag gctcggaatc tgttgcggct tcgcttcgcg gatcaggccc gacagggcga
     5521 tgctgctgaa caggatctcg tgcacggcga cgcgaccgac gccgtcggcc ttgcgcacga
     5581 gctgttgcgc gatgatgccg gccagcgact cggcgagtag accgcgaatc tgcggctgct
     5641 gatctgcgga gaacgcgttg acgtagcgat cgatcgtggc cgatgcggag ttggtgtgca
     5701 cgctggcgaa caccaagatg ccgaagctcg cgagctggag cgcagccttc atggtttcgg
     5761 agtcgcgaag ctcgccgacg aggatcacgt cggggttctc gcgcgacgcg ctgcggatcg
     5821 catcgacgaa gctcggcacg tggtgcccga tctccttgtg cgtgacgatc gccttgcgcg
     5881 atttgtgaat gaactcgatc gggtcctcga tcgtgagaat gcgaaccggc ctcgtgcggt
     5941 tgatctgatc gatcatcgcg gcgagcgtgg tggtcttgcc gctacccgtc ggaccggtga
     6001 cgaggacgag acccgatcgc acttcgacga gcttgcgcac gccttgcggc acagcgagat
     6061 cgtcgagcgt tgcgatcttt gacgggatcg aacggaagac gccgcccacg ccggtcgtct
     6121 tgcggaagta gctcacgcgg aagcggccgc gcgtgtcgta ctgatacgcc caatccaaat
     6181 cgcccgtcgc atcgaacact cgccggtgat gctcctgcag aagcggcatc aacatcgcgt
     6241 cggcatcttc catcgtgagg cgttcgtcgc cgagaggaag gagctcgccg cgcacgcgca
     6301 gtgtcggtgg ataacccggc gcgatgtgaa cgtcagagcc cttgagccga tagagctcct
     6361 cgaacagctc ggcgagtccc atcacgaggt cggcccaccc ttcccgccga acaggccacg
     6421 cgagcttgtc ggtgacggtg tcgtgcggcc tccgaggagc gctgcgaggt cgcgcttgtt
     6481 ctcggccgca ccgagcgcga cttcctcggt gatgcgtttc gcgcggacgt gctcgacgag
     6541 cgaatcgtcg aggcgaatca tgccaaacgc gcggccgcgc tgcatgaggc tcgggagctg
     6601 gaacatcttg ttgtcgcgaa tcatcgtcgc gagcggcagc acgccggtca ccatctcgat
     6661 tgcggcgacc attccgtcct gcgtcgcgtt cggcaggagt cgctgcgcga tcacggcgcg
     6721 aagcgttcca gagatcgatg cgcgcacctg gacttggtcg tcgggcggga acatgtcgat
     6781 caagcgatcg atcgtcttca ccgcggacgg cgtactaatc gtcgacagca cgaggtggcc
     6841 cgtctcggcg gccgcgagcg caatctcgac ggtctcgcga tcgcgaagct caccgatgac
     6901 aatcacgtcc ggatcttcac gcagcgatgc cttcagcgca gacacgaagc tcagcgtgtg
     6961 acgaccgact tcgcgatgcg acacgatcgc gcgcttcgcc ggatgctcaa tctcgacggg
     7021 atcttcgatc gtgatgatgt gatcgctgtt cgacgtattc agcagatcga cgagcgcagc
     7081 catcgtcgtg gtcttgccgt gcccgctcgg gccggcgatg acgacgagtc cctggtgatg
     7141 gccgatcact ttctcgagct cgcgcggcag cccgagcccg gcgagcgtgg gaatcgtgtc
     7201 gcgacacaac cgaaacgacc ccgccagtcc atcctgctgc tgcgagatat tggcgcgaat
     7261 gcgaccgcgg cccggaacct gaaagccgag gtcgacgtaa ccaacctcgc cgaggcgttg
     7321 ccgcgacgac ggcccgagca gcggcagaag gaagcgctcg gcgagcacgg gatcgagcgg
     7381 cccgaacagc gcgctgtaca ccgcgcgatc gaacggctcg gtcagcgctt ttgcctcgag
     7441 cggcgtcagc tggccgagcc gccggatcgt gatgggctga ttcgcaatga tgtgaaggtc
     7501 ggacgcgctt cgccgatacg ccgcctcgag caacgcgtgc agcgacgtga tgtccgtgtg
     7561 ctcgtcgacg atctgcggcc cgtaccactc gggcgcatac acctcggcgg ccgcgacctc
     7621 tggcgtgcca tacgccgcgc ccgatgcgtc ctcggcgtcg tcgacgattt cgccaccgcc
     7681 gccgagcgcc gtcgcgtacg cgagatccaa gtccggtaca tcagcgacga tcccgtcatc
     7741 ctcgagcgaa aagcccggat ccggcgcgct ctgcccgcgc agcggctcgg gcttgtacac
     7801 gtcggtcgtg agccgcggca tcgtcgccgt gctgagcgga gtgacggtca gcggtggcgt
     7861 tgtgccgccg ccgccacggc ccgtcggtgg aatcagcggg ctgcccatcg tcgacgtcag
     7921 cgtgcgcggc ggtgcggtcg tcgtcgcggt ggtgcgcggc gctgcagtcg tcgtcgttgc
     7981 ggtgcgcggc gcgatcgtcg tgccggcgct gcgggcatcg ctgcgcgacg tctcgtcggg
     8041 cagaggcgaa gctgtcgtgc tcgccgcgcg cggcgaaatg ggcgcgcccg tcggcgacat
     8101 catcgtggcg ttgcgtccgg atgtcgacga cggtggcgct gcggtcgaag aggccgtgcg
     8161 cacggagccg acaggcgcgt cgtcgatcgg atcggtcggc agtggcatcg tcgtgcgcgg
     8221 tagcgatgcg gtggtcgcgg ccgcgctcgt cggcgacatc acggtcgcgt tgcgacccgt
     8281 tgtcgacgac ggtggtgctg cggtcgagga ggtcatgcgc actgacacgc cggcgctgtc
     8341 gtcgatcgga gcggtcggca gtggcgtcat cgtgcgcggc gcggatgtcg gcgaggtcgt
     8401 gcgcacggag acgatgggcg cgtcgtcgat cggagccgac ggcggtgggg
//
LOCUS       DATLBZ010000015        11579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1502038, whole genome shotgun sequence.
ACCESSION   DATLBZ010000015 DATLBZ010000000
VERSION     DATLBZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11579
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502038"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VMZ53_00490"
     CDS             complement(<1..489)
                     /locus_tag="VMZ53_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26944.1"
                     /translation="MTSPAARLALLVLVLPGCLDGDVGTDRNAGGKGDVWGTDDRVER
                     YEIQSMALQEVARSEAALVRSSSLVHDPMRDVWSATSPKTLGDAQHLCAGERFADQPV
                     VAYCSATLIADDIMVSAGHCFEAVDCADLLVAFDLAYDAAPTDPMQIAHDMPAANVYR
                     CAE"
     gene            complement(627..2759)
                     /locus_tag="VMZ53_00495"
     CDS             complement(627..2759)
                     /locus_tag="VMZ53_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26945.1"
                     /translation="MSTKWLLGLAVVVAALSRHQVDACGPGFPTNMIVRRADALATMW
                     DGSFTDEAKKLAAVSDADRAAFTAVPTKLDATKRELDLYRDAAAQFHAGDYTLAAKGF
                     EALLRLPAAQRKATSVSAAYSLGRARAELYDADRAIAAFRHVRVLVRAGFVDGDGLAR
                     ASLGEEARIERTQRGNMVAAVHRYAEQAALGDSTGWTSLLFLMQVVTPKARAELYRDD
                     VGTKLLALYYYTRTTWLSDEERPFWKKELLRNATTEARGAGYMAAAMYREGAWDTAAT
                     LASRCVHSPIATWVQAKLALRDGDRTKAEALLRVVERAGLVGNDGTGDQPYTLDGDPR
                     SLVRADLALLALSDGRFVEAARWFARGNRTAEAAYVADRVLTFDELEAEVQRTESARV
                     AGPKLAEADDPEWPCANEGIYADGTLLPDPSYCWSTRLQKLYARRAMREHRFQVALDA
                     VDNEQARALVSTLDRADTSSGIERAEQLFAASRILRAHGLELAGTETGPDWAMYDGQY
                     ARPLLCLPQRAHGYTRFAVPTDDDYGDDYQGEDCLAPSRDDAALVSPLEVSRVRASAP
                     EIDERFSYRYTASRLAEQAAELVPPRSQAYAAALCWAARFAHRDQTRVEELYAKYNRN
                     GAAGALTGLFAEDCDEPDFHAARDFDERQAERRMQRAVTEARSHAWTWPRIRRAAWRR
                     RRWVLVPLAFLVALLALLVSRRRLPRET"
     gene            complement(2749..3837)
                     /locus_tag="VMZ53_00500"
     CDS             complement(2749..3837)
                     /locus_tag="VMZ53_00500"
                     /inference="COORDINATES: protein motif:HMM:NF022784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HUS26946.1"
                     /translation="MSIGRALALLVIVAACDRNRAAAPKPAVPAHQDAYVWQRSWTPA
                     VRDAVEHAPAKIEGLRILAAEVAVGDPLHAISLDAPSLAASGRRVTLVLRINGSRPMD
                     DLSIAPLLATAQTLQQAGVDVAGIEVDHDCATARLAAYARWLALQDHASLRLSITALP
                     TWAESPALRDVATAVDEIVVQVHAVRAPAIFDPNVAWHDLVRFSREVRGTPLRVALPT
                     YGAVVRGMEVEADVRDVAAFERRLAAESIVDGVVWFRLPVKGDPQTWSPATFAHVVER
                     GTPDAPQAKLALVARGADQFDVVARNEGTELVDLPPVRVAGAIDAADMIGYRAVTQHE
                     WAAPHRSLAPSESITIGWIRGKDITLVD"
     gene            3953..4747
                     /locus_tag="VMZ53_00505"
     CDS             3953..4747
                     /locus_tag="VMZ53_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HUS26947.1"
                     /translation="MLRPLLLVFALACTMAAGCGAKSGTASVDYSVSAQKNYEKGLKE
                     LERQDWVAASKYFGFIKSRFPYSKYAVLAELRLADAEFGAEQYLEAIDSYRLFIKFHP
                     THEMVANGYASFRIGEAYYKQLPGDFWLFPPSYEKDQSATEDAVTELKSFLDKYPDSP
                     YRDKAKTILVKVGKRLADHEWYVARYYWDRGKPMGTVLRLRRLLESYRGVGYDEEALW
                     LLGRAYVAVQMPDRAKTAWTELVQKFPKSDRAGDARDALASLSPKR"
     gene            4763..5500
                     /locus_tag="VMZ53_00510"
     CDS             4763..5500
                     /locus_tag="VMZ53_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS26948.1"
                     /translation="MSADRVEQLIALGREHYNANEYDKAEPFLAEAAAARPSFPDLYN
                     MLGVIYHAQGRFSDAEEAFENALRLNPRYTEAALNLSVTYNDRGKYERARDVYTRAVS
                     ASVGQPNALDRFARGKLANMHADLGAAYAGLAMFDEAVREYSKALDLCSDFADLRVRL
                     GNVYRDMGMFHAAVAEFEHAKKLRPDFVPARIHLGVTMFSLGRRDAAISEWQEVLKID
                     PSNKSAQLYIRMCQDENGPQPGSALPR"
     gene            complement(5501..6520)
                     /locus_tag="VMZ53_00515"
     CDS             complement(5501..6520)
                     /locus_tag="VMZ53_00515"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HUS26949.1"
                     /translation="MRAGCVALAFLVACGGHYGGKPNAAKPADTKGGIERAALPYAIL
                     DARTGRQVDTEQFWQQLGTQRVVCVGEDHQNPHHHWVQLEVMSQLAAHHKGKKLALGM
                     EMFQRPFQGVLDDYAAKRIDEATLLSRSGWDDRWGYDYGFYGPTIRAAVAVGAALVAL
                     NAPRELTKAVSHKGIAGLAPDEKAQLPEVNLDDKSHRAWFDSLMEGMGGAGGHTKAPE
                     EAKPDDTKPDDKAADKLPEGHPAMPPREMPSADNIYAAQVVWDESMADGAAKWAQANA
                     TGEMVLLAGNGHCHDSAIVNRVKRRGVAQVVSVRPVLDTEGNVAEALAKPMNDYLVVL
                     QLPKQ"
     gene            6556..7578
                     /locus_tag="VMZ53_00520"
     CDS             6556..7578
                     /locus_tag="VMZ53_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HUS26950.1"
                     /translation="MDLPYLTAELPGIAGTLRTTPEDFFVDEEAAYEPSGTGDHVFVR
                     IEKRGLTTMQAVERIAKAVSVNPRDIGVAGMKDRHAVTRQWISLPPPVTPETTRALVL
                     DDIAICDVARHGHKLRTGHVRANRFILRVRGTADDAVTKAQAIFDVLAKPPGTPNWYG
                     EQRFGRDGDNAERGLAIVRGERPPRDKKLARLLVSSLQSKLFNEWLTARMRDGLYARV
                     LAGDILHKIPGGQFVCEAPDADQPRLLAGEVGITGPMFGDSMRAPPAGSEAAAREAQI
                     LDAAGLAADSFAGVSRIAEGTRRDAAVAVGNPSVRAVEEGIEVAFTLPGGAYATAVMR
                     EVMKSG"
     gene            7590..8231
                     /locus_tag="VMZ53_00525"
     CDS             7590..8231
                     /locus_tag="VMZ53_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HUS26951.1"
                     /translation="MLSELFVAFAQVGAEWVLWLLIGLSVLSVGIMIDRAIWFQKRDM
                     DPEAFTRELRGAFERNELERLETKYKNDPSVPVQVALRGLAERDRGPEAVAEAMQGER
                     VRWRNAGDKHLIILGTLGNNVPFLGLFGTVLGVINAFENLRAKTAAAEDRTLDLIAQA
                     LAATAFGLLVAIPAVIAFNYFTRKMRTVLGSADVAAHTVLAMVHGQAHTKDAK"
     gene            8231..8647
                     /locus_tag="VMZ53_00530"
     CDS             8231..8647
                     /locus_tag="VMZ53_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HUS26952.1"
                     /translation="MAGGSLYQDGDESAPITDINVTPFVDVVLVLLVVFIVTAKLIVA
                     RGVDIDKPKAATGGEVQSTLRVSVTADGQLFVNGDAFVDDAKAIERIKTIASTTSKPK
                     AIIAGDRKTAYGGVMRAIDLVQQAGVTAIALENERQ"
     gene            8687..9484
                     /locus_tag="VMZ53_00535"
     CDS             8687..9484
                     /locus_tag="VMZ53_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HUS26953.1"
                     /translation="MRDSVANATIGSIAIHVGLATLAYTLLVTRPVLPPPKVPKVDLV
                     EIEPPPLIKPLPPPVQPETPKPPEAAPEPVKQPVQQRATKAVTRPATTPPPTTEQPPP
                     TTTTSDDSGGAPVTTMEDIAPSATGVAVAKGPRTTGKVGRGGTGGGTGSGAGTGSSDA
                     PPTPVSVATIKTRAMPKGDFSYVDASKDYPAEAKALGIEGQIRVRLIVDDAGAVKSAV
                     LLNKLGHGLDELALERAKKIVFDPAKDTDDKPVSSVVIWTFNMTLPK"
     gene            9535..>11579
                     /locus_tag="VMZ53_00540"
     CDS             9535..>11579
                     /locus_tag="VMZ53_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HUS26954.1"
                     /translation="MHKLWAACLFAAALVLPPNLAHAQAMDPPADRAIDINNFDYSIG
                     PKSFLTVDNADTADKKQLALDAMFTYLTRPFTVYNTDGANDPTITNERTEVIKSYSAA
                     QLTAAYGINEKLQVGANLPLIFSLSGDGLDPNTGRGVMEGVQVTGLGDLLVEGKYRLW
                     QNRTGELRLAGIGGVTLPSSFGSDDSQFIGDNLPTLRGRAAATWSKGRIALGATAGFI
                     LRKPRTIYSSTIGQQLTFGVGANVQATNNFSVIGEFYGRGGLESGFSLDESPMEAVGG
                     IRVIAAKSVAVTIGGGAGLDRAIGSPQARFFLSLGYAPDVRDSDGDGIQNARDKCPLI
                     PEDRDGADDGDGCPDDDNDGDRRPDTEDKCPAAAEDLDGFDDDDGCPELDNDGDKMDD
                     LKDKCPNDAEDGKDPYPTDGCPGNKRDSDADNIPDAVDSCPMEEEDTDSFEDGDGCPE
                     ADNDADGVADANDKCPVCPEDKDTFEDEDGCPELDNDKDGVPDAKDACPAQAESFNGI
                     KDDDGCPDTGGVELVKLDGDRLEIGKVPTMDKKGLTKAGDQIVAQIAGVMIAHNEVTK
                     WLIALAQPKQEDAQRLAGYINDRLAKAGVTNAQVLGAQGPSKIGGVVQERSEGGDTTA
                     APVCPAGREVKQRPEMIEPKATMNRPTVQAPPTVEPKKKDEPKKDEPKKDGDEIEMEP
                     "
BASE COUNT         2054 a         3907 c         3827 g         1791 t
ORIGIN      
        1 ttcggcgcag cggtaaacgt ttgcagccgg catgtcgtgc gcgatctgca tcggatcagt
       61 tggcgctgcg tcgtaggcga gatcgaatgc gacgagaaga tcagcgcagt caacggcttc
      121 gaagcagtga ccggcggaga ccatgatgtc gtcggcgatc aaggtcgcgg agcaatacgc
      181 gacgaccggt tgatcggcga atcgttcgcc ggcgcagagg tgctgcgcat ccccgagcgt
      241 cttcggtgac gttgccgacc acacgtcgcg catcgggtcg tgaacgagcg agctgctgcg
      301 aacgagggcg gcttcgctgc gagctacctc ctgcagcgcc atcgactgga tctcgtaacg
      361 ctcgacgcga tcgtcggtgc cccatacgtc gcccttgcct cctgcgttgc gatccgttcc
      421 cacgtcgccg tcgaggcagc cggggagcac gaggacgagc aacgcgagac gagcggccgg
      481 tgacgtcatg cgagccagtt catcaacgtg cgtgccaccc gtgcctcgtg gcatcccgtg
      541 tggttagcgc gcgatcgtag cagccatggc aagcgagcta gtcgcaaggt ggcgcaagcg
      601 gtgacacgcg ctggcgcagc tcgcgctcag gtctcgcgcg gtaaacggcg tcgagatacg
      661 agcaacgcga gcaacgcgac gagaaatgcc agcgggacaa gcacccatcg gcgtcggcgc
      721 cacgccgctc gacggatgcg cggccatgtc cacgcgtggg agcgtgcctc cgtgactgcg
      781 cgctgcatgc ggcgctcggc ctgacgctcg tcgaaatcgc gcgccgcgtg gaagtcgggc
      841 tcgtcgcagt cttccgcgaa cagccccgtc agcgcgcccg cagctccgtt gcggttgtac
      901 ttcgcgtaca gctcttcgac gcgcgtttga tcgcgatgtg cgaagcgcgc cgcccagcac
      961 agcgccgctg cgtacgcctg actgcgcggc gggacgagct cggccgcttg ctcggcgagg
     1021 cgcgacgcgg tgtagcgata cgagaaccgc tcgtcgatct ctggagcgct cgcgcgaacg
     1081 cgtgacacct cgagcggcga cacgagcgct gcgtcgtcac gcgacggagc gagacagtct
     1141 tcgccttgat agtcgtcgcc atagtcgtcg tcggtcggaa cggcgaagcg cgtatagccg
     1201 tgcgcgcgtt gtggcaggca cagcagcggg cgcgcgtact ggccgtcgta catggcccag
     1261 tccggtcccg tctcggttcc cgcgagctcg aggccgtgcg cccgcaggat ccgcgacgcg
     1321 gcgaacagct gctcggcacg ttcgatgcca ctgctcgtgt cggcgcggtc gagcgtgctg
     1381 acgagggcgc gtgcttgctc gttgtcgact gcgtcgagcg cgacttggaa gcggtgctca
     1441 cgcatcgctc gccgcgcata gagtttctgg aggcgcgtgc tccagcaata ggacggatct
     1501 ggcagcagcg ttccgtcggc gtagatgcct tcgttggcgc acggccattc tggatcgtcg
     1561 gcctcggcga gcttaggacc ggcgacgcgc gcgctctcgg tacgctgaac ctcggcttct
     1621 agctcgtcga acgtgagcac gcggtcggcg acgtatgcgg cttcggcggt gcgattgccg
     1681 cgcgcgaacc atcgtgcggc ctcgacgaag cggccgtcgg agagcgcgag cagtgcgagg
     1741 tcggcgcgca cgagcgaacg cggatcgccg tcgagcgtgt acggctggtc accggtgcca
     1801 tcgttgccga cgagccccgc ccgctcgacg acacgcagca gagcctcggc cttcgtgcgg
     1861 tcgccgtcgc gcaacgcgag cttcgcctgg acccacgtcg cgattggcga atgcacgcag
     1921 cggctcgcga gcgtcgcagc agtatcccac gcgccctcgc gatacatcgc tgccgccatg
     1981 tagcccgcgc cgcgcgcctc ggtcgtcgcg ttgcgaagca gctccttctt ccagaacggc
     2041 cgttcctcgt cggagagcca cgtggtgcgc gtgtagtagt agagcgcgag caatttcgtt
     2101 ccgacgtcgt cgcgatacag ctctgcacgc gccttcggcg tcacgacctg catgaggaac
     2161 agcagcgacg tccagccggt gctgtctccg agagccgcct gctctgcata gcgatgaacg
     2221 gccgcgacca tgttgccccg ctgcgtgcgc tcgatgcgcg cctcttcgcc caggctcgcg
     2281 cgtgcgagtc catcgccgtc gacgaagcct gcgcgaacga ggacgcgtac gtgccggaat
     2341 gctgcgatcg cgcgatcggc gtcgtagagc tcagcgcgcg ctcgaccgag cgaatacgcc
     2401 gcggacaccg aagttgcctt tcgctgcgcc gcgggcaatc gcagcagcgc ctcgaagccc
     2461 ttcgccgcta gcgtgtagtc gccagcgtga aattgcgcgg ctgcgtcgcg atagaggtcg
     2521 agctcgcgct ttgttgcgtc gagctttgtg ggcaccgcgg tgaatgcggc gcgatcggcg
     2581 tcggacaccg cggcgagctt ctttgcttcg tccgtgaacg agccatccca catcgtcgcg
     2641 agcgcatcgg cacgccgcac gatcatgttc gtcggaaatc cggggccaca cgcgtcgacc
     2701 tggtgccgcg agagcgcagc gacgacgacg gcgagcccga ggagccactt agtcgacaag
     2761 cgtgatgtcc tttccgcgaa tccaaccgat ggtgatgctc tcgcttggcg cgagcgatcg
     2821 atgcggagct gcccactcgt gctgcgtgac cgcgcgatag ccgatcatgt ccgccgcatc
     2881 gatcgcaccg gcgacgcgca cgggcggcag atcgacgagc tcggtgccct cgttgcgcgc
     2941 gacgacatcg aactgatcgg caccgcgtgc aacgagcgcg agcttggcct gcggcgcgtc
     3001 cggcgtgcct cgttcgacga catgcgcgaa cgtcgccggc gaccacgtct gcggatcgcc
     3061 cttcacgggc aaccggaacc acacgacacc gtcgacgatc gactcggctg cgaggcgacg
     3121 ctcgaaggcc gcgacgtcgc gcacgtcggc ctcgacctcc atgccacgaa ccaccgcacc
     3181 gtacgtcggc aacgcgactc tcagcggcgt tccgcggact tcacgcgaga accgcacgag
     3241 gtcgtgccac gcgacgttcg ggtcgaagat cgcaggagca cggacggcgt ggacctggac
     3301 gacgatctcg tcgaccgccg tcgcgacatc acgcagcgcg ggcgactccg cccatgtcgg
     3361 cagcgccgtg atcgagagtc gcagactcgc gtggtcctgc agcgcgagcc accgtgcgta
     3421 ggcggcgagg cgcgcggtcg cgcaatcatg atctacttcg atacctgcaa cgtcgacacc
     3481 ggcctgctgc aacgtctgcg cggtggcgag gagaggcgcg atcgagagat cgtccatcgg
     3541 ccgcgagccg ttgatccgca ggacgagcgt gacgcgccgg ccgctagccg cgagtgacgg
     3601 ggcatcgagc gagatcgcgt gaagcggatc gccgaccgcg acctccgccg ccaaaatgcg
     3661 aagcccttcg atcttcgcgg gcgcatgttc gacggcatcg cgcactgctg gcgtccagct
     3721 gcgctgccag acgtacgcat cctgatgggc gggaacggcc ggtttcggcg ccgccgcccg
     3781 attccgatcg cacgccgcca cgatgacgag cagcgcgaga gccctcccaa tggacatcct
     3841 tttgcgcgaa cgcgcaacga cgcgaaccta ttgcgggcgc gacgtctaac tgcaagcgtt
     3901 tgcaggcaac ccttgtggcg gcggccatgt cacagtacac acggacgtcc tgatgctccg
     3961 cccccttctc ctcgtgtttg cgctggcttg caccatggcc gcgggctgcg gcgcgaagtc
     4021 tggcaccgcg tcggtcgact actccgtctc ggcgcaaaaa aactacgaga agggcctcaa
     4081 ggagctcgag cgccaggact gggtcgccgc ctcgaagtac ttcggcttca tcaagagccg
     4141 gttcccgtac tcgaagtacg cggtgctcgc cgagctccgc ctcgccgacg ccgagttcgg
     4201 cgccgagcag tatctcgaag cgatcgacag ctatcgcctg ttcatcaagt ttcacccgac
     4261 gcacgagatg gtcgcgaacg gctacgcgtc gttccgcatc ggcgaggcgt attacaagca
     4321 gctccccggc gacttctggc tgttcccgcc gtcgtatgaa aaagatcagt cggcgaccga
     4381 agacgcagta accgagctga agagcttcct cgacaagtat ccggactcgc cgtatcgcga
     4441 caaggcgaag acgatcctcg tgaaggttgg caagcgcctc gccgatcacg agtggtacgt
     4501 cgcgcgctac tactgggatc gcggcaagcc gatgggcacc gtgctgcggc tgcgtcgctt
     4561 gctcgagagt tatcgcggcg tcggttacga cgaagaagcg ctgtggctgc tcggccgtgc
     4621 gtacgtcgcg gtgcaaatgc cggatcgcgc gaagacggcg tggacagagc tcgtgcagaa
     4681 gttcccgaag agcgatcgcg caggcgatgc ccgcgatgcg ctcgcgagcc tttcgcccaa
     4741 gcgctgaggt acgctagcgc ggatgagcgc tgaccgcgtc gagcaactga tcgcgctggg
     4801 tcgcgagcat tacaacgcga acgagtacga caaggccgag ccgttcctcg ccgaagcagc
     4861 ggccgcgcga ccgagcttcc ccgacctcta caacatgctc ggcgtcatct atcacgccca
     4921 aggccggttc tccgacgccg aagaagcgtt cgagaacgcg ctgcggctca accctcgcta
     4981 caccgaggcg gcgctgaacc tcagcgtgac ctacaacgat cgcggcaagt acgagcgcgc
     5041 gcgtgatgtc tacacgcgag ccgtgtccgc gtcggtcggg cagcccaacg cgctcgatcg
     5101 gttcgcgcgc ggcaagctcg ccaacatgca cgcggacctc ggtgcggcgt acgccggcct
     5161 cgcgatgttc gacgaagcag tgcgcgagta ctccaaggcg ctggacctct gctccgactt
     5221 cgccgatctg cgcgtgcggc tcggcaacgt ctatcgcgac atgggcatgt tccacgcggc
     5281 ggtcgccgag ttcgagcacg cgaagaagct acggcccgac ttcgtaccgg cgcgcatcca
     5341 cctcggcgtg acgatgttct cgctcggccg tcgcgacgcc gcgatcagcg agtggcagga
     5401 agtcctcaag atcgatccgt cgaacaagag cgcgcagctc tacatccgca tgtgccagga
     5461 cgagaacggc ccgcagccgg gctccgccct accccgctga ttactgcttc ggcagctgca
     5521 gcacgacgag gtagtcgttc atcggcttcg cgagcgcctc ggcgacgttg ccttcggtgt
     5581 cgagcaccgg ccgcacggac acgacctgcg cgacgccgcg ccgcttcaca cgattgacga
     5641 tcgcgctgtc gtggcaatgc ccattgccgg cgagcagcac catctcgccc gtcgcgttcg
     5701 cttgcgccca cttcgcggca ccgtccgcca tcgactcgtc ccacacgacc tgcgctgcgt
     5761 agatgttgtc ggcggacggc atctcgcgcg gtggcatggc cggatgacct tccggcagct
     5821 tgtcggctgc cttgtcgtcg ggcttcgtgt cgtccggctt ggcctcttcg ggcgccttcg
     5881 tgtggccgcc ggcgccgccc atgccttcca tcagcgagtc gaaccacgcg cggtgtgatt
     5941 tgtcgtcgag gttcacctcg ggcaactgcg ctttttcatc gggcgcgagg cctgcgatgc
     6001 ctttgtgcga gacggctttg gtcagctcgc gaggtgcgtt gagcgcgacg agcgctgcac
     6061 cgacggcgac ggccgcgcga atcgtcgggc cgtagaagcc gtagtcgtac ccccagcgat
     6121 cgtcccaacc ggagcgcgag agcagcgttg cttcgtcgat gcgcttcgcg gcgtagtcgt
     6181 cgagcacgcc ttggaacggg cgctgaaaca tctccatgcc gagcgcgagc ttcttgccct
     6241 tgtgatgcgc ggcaagctga ctcatcacct cgagctgcac ccagtgatgg tgcgggttct
     6301 ggtgatcctc gccgacacat acgacgcgct gcgtgccgag ctgctgccag aattgctcgg
     6361 tatcgacttg gcgcccggtg cgcgcgtcga ggatcgcgta cggcaatgcg gcgcgctcga
     6421 tgccgccttt cgtatcggcg ggctttgcgg catttggctt accgccgtaa tgcccgccgc
     6481 aggcaaccaa gaaagcgagt gcaacgcagc ctgcacgcat gagagcgatg ctaacaccgg
     6541 cagcgagcgc gcgttatgga cctgccttac ctcaccgcag agctgccggg catcgctggc
     6601 acgctgcgca cgacccccga ggacttcttc gtcgacgagg aagctgcgta cgagccgtcg
     6661 ggcaccggcg accacgtctt cgtgcgcatc gagaagcgcg ggctgacgac gatgcaggcg
     6721 gtcgagcgga tcgcgaaggc cgtgagcgtg aacccgcgcg acatcggcgt cgccggcatg
     6781 aaagatcggc acgcggtcac ccggcagtgg atctcgctgc cgccgccggt cacgccggag
     6841 accacacgcg cgctcgtgct cgatgacatc gcgatctgcg acgtcgcgcg ccacggccac
     6901 aagctgcgca cgggtcacgt gcgagcgaat cgctttatcc tgcgcgtgcg cgggaccgcc
     6961 gacgatgcgg tcacaaaagc gcaggcgatc ttcgacgtgc tcgcgaagcc gcccggcacg
     7021 ccgaactggt acggcgagca gcgcttcggc cgcgacggtg acaatgccga gcgaggcctc
     7081 gcgatcgtgc gcggtgagag gccgccgcgt gacaagaagc tcgcacgcct gctcgtctcg
     7141 tcgctgcaga gcaagctgtt caacgagtgg ctcacggcgc gcatgcgcga tggcctctac
     7201 gcgcgcgtcc tcgcgggaga catcctccac aagattcccg gcggccagtt cgtgtgcgaa
     7261 gcgcccgatg ccgatcagcc gcgcctcctg gcgggagagg ttggcatcac cgggccgatg
     7321 ttcggcgaca gcatgcgtgc gccgccggcg ggcagcgaag ccgccgcgcg tgaagcccag
     7381 attctcgacg ctgcgggtct cgccgccgat tcgtttgcgg gcgtgtcgcg catcgcagag
     7441 ggcacgcgac gagatgccgc ggtcgccgtg ggcaaccctt ccgtgcgtgc agtcgaagaa
     7501 ggcatcgaag tggcattcac gctgccgggt ggcgcgtacg cgaccgccgt catgcgcgag
     7561 gtcatgaaat cgggctaagg tccggctcga tgctgtcgga gctattcgtc gcgtttgccc
     7621 aggtcggtgc cgaatgggtg ctgtggctcc ttatcgggct ctcggtgctc tcggtcggca
     7681 tcatgatcga ccgcgcgatc tggtttcaga agcgcgacat ggaccccgag gcgttcacgc
     7741 gcgagctgcg cggcgcattc gagcgcaacg agctcgagcg gctcgagacg aagtacaaga
     7801 acgatccgtc ggtgcccgtg caggtcgctc tgcgcgggct cgccgagcgc gatcgcggcc
     7861 ccgaggccgt cgccgaagcg atgcaaggtg agcgagtgcg gtggcgcaat gcgggcgaca
     7921 agcacctcat cattctcggc acgctcggca acaacgttcc gttcctcggc ctgttcggca
     7981 cggtgctcgg cgtgatcaac gcattcgaga acctccgcgc gaagaccgct gccgccgaag
     8041 accgcacgct cgacttgatc gcacaggcgc ttgccgcgac cgcgttcggt ctgctcgtcg
     8101 caattcccgc cgtcatcgcg ttcaactact tcactcgcaa gatgcgcacg gtcctcggca
     8161 gcgcggacgt cgcggcgcac accgtcctcg cgatggtcca cggccaggcg cacacgaagg
     8221 acgcgaagta gtggccggcg gaagcctcta ccaagacggc gacgagagcg cgccgatcac
     8281 ggacatcaac gtcacgccat tcgtcgacgt cgtgctcgtg ctgctcgtgg tgttcatcgt
     8341 gacggcgaag ctcatcgtcg cgcgcggcgt cgatatcgat aagcccaagg cggcgaccgg
     8401 tggcgaagtg cagagcacgc tgcgggtgtc ggtcaccgcg gacggccagc tgttcgtcaa
     8461 cggcgatgca ttcgtcgacg acgcgaaggc gatcgagcgg atcaagacca tcgcgtcgac
     8521 gacgagcaag cccaaggcga tcatcgccgg cgatcgcaag accgcgtatg gcggcgtcat
     8581 gcgcgcgata gacctcgtgc agcaggcggg tgtgaccgcc atcgcgctcg agaacgaacg
     8641 ccagtagccg accggcaatc gtccggcgat acgctaggct agaacggtgc gcgattcggt
     8701 ggccaacgcg acgatcggat cgatcgcgat ccacgtgggt ctcgcgaccc tcgcgtacac
     8761 gctgctggtg acgaggcccg tgctgcctcc gccgaaggtt ccgaaggtcg acctcgtcga
     8821 gatcgagccg ccgccgttga tcaagccgct gcctccgccg gtgcagcccg aaacaccgaa
     8881 gccgcctgaa gctgcgcccg agccggtgaa gcagcccgta cagcaacgcg cgaccaaagc
     8941 ggtcacgcgc cccgcgacca ccccgccgcc gacgaccgag cagccaccgc cgacgaccac
     9001 gacgagcgat gactcgggcg gtgctcccgt cacgacgatg gaagacatcg ccccgtcggc
     9061 caccggcgtc gcggtcgcga aaggccctcg caccacgggc aaggtcggtc gcggcggaac
     9121 cggcggcggg acgggtagcg gcgcgggtac aggatcttct gatgcgcctc ccacgccggt
     9181 gtcggtggcg acgatcaaga cccgggcgat gcccaagggc gacttcagct acgtcgacgc
     9241 gagcaaggac taccccgccg aagccaaagc cctgggaatc gaaggccaga tccgggtgcg
     9301 gctcatcgtc gacgacgccg gcgcggtcaa gagcgcggtg ttgctcaaca agctaggcca
     9361 cggcctcgac gagctcgcgc tcgaacgcgc caagaagatc gtcttcgatc cggcgaagga
     9421 caccgacgac aagccggtct cgtcggtagt gatctggacg ttcaacatga ccctcccgaa
     9481 atagctatcc cgggggtacc ccgtcgccgg gcgtgtcggt ttaagatgct gacaatgcac
     9541 aaactatggg ctgcgtgtct atttgcggcc gcgctcgtgc tgccgccgaa tctggcgcac
     9601 gcgcaagcca tggacccgcc cgccgaccgc gcgatcgaca tcaacaactt cgattactcg
     9661 atcggaccga agtcgttctt gacggtcgac aacgcggaca ccgctgacaa gaagcagctc
     9721 gcgctcgacg cgatgttcac gtacctgacg cgtccgttca cggtctacaa caccgacggc
     9781 gcgaacgacc cgacgatcac gaacgagcgc accgaggtca tcaagtcgta cagcgctgcg
     9841 cagctcaccg ccgcgtacgg catcaacgag aagctgcaag tcggcgcgaa cctcccgctg
     9901 atcttctcgc tctccggcga cggtctcgat ccgaacacgg gccgcggcgt gatggaaggc
     9961 gtgcaagtca ccgggctcgg cgatttgctc gtcgagggaa agtatcgcct ctggcagaac
    10021 cgcaccggcg agctgcgcct cgcgggcatc ggcggcgtca cgctgccgtc gagcttcggc
    10081 agcgacgact cgcagttcat cggcgacaac ctgccgacgc tgcgtggtcg cgccgcggcg
    10141 acatggtcca aaggcaggat cgccctcggc gccaccgcgg gcttcatcct gcgcaagccg
    10201 cgcacgatct actcgagcac gatcggtcag caactcacgt tcggcgtcgg cgccaacgtg
    10261 caagcgacga acaacttctc cgtcatcggc gagttctacg gccgcggcgg tctcgagtct
    10321 ggcttctctc tcgacgagag cccgatggaa gccgtcggcg gcatccgcgt gatcgcggcg
    10381 aagtccgtcg cagtaacgat cggcggcggc gccggtctcg atcgcgccat cggctctccg
    10441 caagcgcgct tcttcctctc gctcggctac gcccccgacg tccgcgacag cgacggtgac
    10501 ggcatccaaa acgcgcgcga caagtgcccg ctcattcccg aagaccgcga cggcgcggac
    10561 gacggtgacg gctgccccga cgacgacaac gacggcgatc gtcgccccga caccgaagac
    10621 aagtgtcccg ccgccgccga agacctcgac ggcttcgacg acgacgacgg ctgccccgag
    10681 ctcgacaacg acggcgacaa gatggacgac ttgaaagaca agtgtccgaa cgacgccgaa
    10741 gacggcaagg atccataccc gaccgacggc tgccccggga acaagcgcga cagcgacgct
    10801 gacaacatcc ccgacgcggt cgactcctgc ccgatggagg aagaggacac cgacagcttc
    10861 gaagacggtg acggctgccc cgaggccgac aacgatgctg acggcgttgc cgacgcaaac
    10921 gacaagtgtc cagtctgccc ggaagacaag gacacgttcg aagacgagga cggctgcccc
    10981 gagctcgaca acgacaagga cggcgtgccc gacgccaaag acgcgtgccc cgcccaagcc
    11041 gaaagcttca acggcatcaa ggacgatgac ggctgccccg acacgggtgg cgtcgagctc
    11101 gtgaagctcg acggcgatcg cctcgaaatc ggcaaagtgc cgacgatgga caagaaaggc
    11161 ctgaccaagg ccggcgacca gatcgtcgcg cagatcgcag gcgtgatgat cgcgcacaac
    11221 gaagtcacga agtggctcat cgcgctcgcg caacccaagc aagaggacgc gcagcgcctc
    11281 gccggctaca tcaacgatcg cctcgcaaag gcgggcgtga ccaacgccca agtcctcggt
    11341 gcccagggcc cgtcgaagat cggtggcgtc gtgcaagagc gctccgaggg tggcgacacg
    11401 acggctgcgc ccgtctgccc ggcaggtcgc gaggtcaagc agcgccccga gatgatcgag
    11461 cccaaggcga cgatgaatcg cccgacggtg caggcgcctc cgacggtcga gccgaagaag
    11521 aaggacgaac cgaagaagga cgagccgaag aaagacggcg acgagatcga gatggagcc
//
LOCUS       DATLBZ010000016        12954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_757879, whole genome shotgun sequence.
ACCESSION   DATLBZ010000016 DATLBZ010000000
VERSION     DATLBZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12954
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757879"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="VMZ53_00545"
     CDS             complement(<1..1478)
                     /locus_tag="VMZ53_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS26955.1"
                     /translation="MSEAANALVGTHIGRYRLVGVLGKGGMGCVYEAVNEASGTRVAV
                     KVIFEAYADDPEMAKRFFAEARAANAIRHEGIVNVIDVVTLPDRRPAIVMELIDGKTL
                     REAVADGAGLATIVQLIGEVLDALAAAHAAGIIHRDLKPENILVTRGGHAKILDFGIA
                     KMLTPIGGRSLPRTRTGAALGTPEYMSPEQISGGDIDGRTDVYAMGVVLFEAVTGRRP
                     FEGATDFELMRAHLEKPAPSAREWSPDLPGVFDDVIARALEKNPRARFQSAKEMASAL
                     RAAERGVPTPRLMEARTVAARRGARPSPSPEASTVGDRPPRSRSRLVVVAAFASVAVA
                     AALVVVSLQAGTKPAARPPDAAVVVEREREPSDAALREVPEDAYVVAVIDEPPDAAPP
                     DARKRDARDASITRSDATTRIDASEADSKQIGVLIWDDPKHRRAFAHESYNVHRLDPL
                     QVLPKARALAKQFAADAVLRGINAGGVTADGYVDLAPETIRYA"
     gene            complement(1502..2815)
                     /locus_tag="VMZ53_00550"
     CDS             complement(1502..2815)
                     /locus_tag="VMZ53_00550"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS26956.1"
                     /translation="MGKPPAVMTRPTERPRRAIQQFRVMVSAGREKGKVWKQLSERCA
                     IGSHPSNDLVIDDPTVSRFHCELAIAGSAVRVRDLGSRNGTLAGDIALTDAMVSGGTV
                     LVLGESAVRIDVASQATELAASERPAFGALIGESAVMREVFSQLEKIAATEATVLIEG
                     ETGTGKEGVADAIHEASARARGPFIVVDCSAIPANLLESELFGHEAGAFTGATQRRIG
                     AFEQASGGTLFLDEIGELPSELQPKFLRALESREIRRVGSSTAVRCDVRIVSATNRDL
                     RTEVNRGTFRADLYYRLAVVRVSLPPLRERIGDIPPLVRHLLAKIGASDSTVTELTQP
                     EFLSALAAAPWPGNVRELRNHIEQCAVFGERRLPNTPSNAHPAVKVDADASYEIARRQ
                     ALDEFERTYLAKLLERHGGNVASAARSAGVNRAYLYRMLGRHGLR"
     gene            2899..3468
                     /locus_tag="VMZ53_00555"
     CDS             2899..3468
                     /locus_tag="VMZ53_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26957.1"
                     /translation="MTTLSSAGWVVHEVGLATSIGGTLFGRAALEPSLSEITSSEERD
                     RVSASAWQRFSWLNLASHAAFAIPWFIGRSMLSGNEVTCEARRLTLVKDVLVGASLIT
                     GVSSILLGRWLGKRADAGQGPAEARDRMSPEGEAEGEAAKTQKLQRAVGLLGNANIAA
                     NIGVLGVTTLLAMQGNKSQRFSFFSRFLP"
     gene            complement(3469..4185)
                     /locus_tag="VMZ53_00560"
     CDS             complement(3469..4185)
                     /locus_tag="VMZ53_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26958.1"
                     /translation="MRIVLLLLLGACGRLSFDPSVADSGLAVPDAPAVVDCSTLPNGA
                     ACDDRNVCTTTSSCRAGACVPEMPQASCLLAASDADFVNDVQGANGWTYGFWAAETDP
                     SYEPATDFVLGVWDTNVFVPPNNVSPFLYLAWWGTHPDAGPSNVTVRRWTSNAQGPAK
                     VRVQVSKGDTSCGDGVGATLVVDGTSRWHRAIAFDNGTGFDDVVDVELAVGTFVELHV
                     AAGPDDSCDTTDSTVTISSP"
     gene            4186..4467
                     /locus_tag="VMZ53_00565"
     CDS             4186..4467
                     /locus_tag="VMZ53_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26959.1"
                     /translation="MRSRIITLLLVVVIGVVGWGVYSNVLSDDTAVRQLAEARARAVA
                     GCGAKCVVKKLEGSRGLLNEQLQFEFTEGSKILVECKRAYIAFGAYTCR"
     gene            4688..5671
                     /locus_tag="VMZ53_00570"
     CDS             4688..5671
                     /locus_tag="VMZ53_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HUS26960.1"
                     /translation="MMMKRAFALGLVAIPACTAGSSGTASVDQSIIGGTTTQTADWKS
                     VVALEDNPGDWFCTGTLITDQWVLTAAHCMEGETPAKLHVRFDTNDANNGTNGHAVAV
                     AEIHSNPAFNFDAWDNDVALIKLAAPVTDRTPTPINRDAIAFGSQVVDVGYGVSDNND
                     GGGGILRKVSKVTADCAGANDTGIVNDNLVCMDASDGRGSCFGDSGGPTFVTVGGALQ
                     VAGVTSGGTGDACGAGWDLYTSVHGELAFIDMIMSAPSDPGGGDGSGSGEGSGSGSGS
                     GMDTNDPSNPGNGEDGGCSAAGGGSSSALLVFALAAVIRRRRRATDAKPAE"
     gene            complement(5646..6239)
                     /locus_tag="VMZ53_00575"
     CDS             complement(5646..6239)
                     /locus_tag="VMZ53_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUS26961.1"
                     /translation="MTERWTTWANAVTLFRLVACVAIFGYARACADETANLVGLVAYW
                     ILDVADGALARWLDQETRIGAQLDILVDRVLVAFFYFNYATFYPQLLVPVCLFLVQFM
                     FIDHFLSNQFMRWRLKSPNYFYEIDREIWRWNWSPFAKAVNTAAVTGVLVVSKSATAG
                     TIVCGAMLVLKTWSTYRVTRLPAPEEGWPATPPASRP"
     gene            6507..7226
                     /locus_tag="VMZ53_00580"
     CDS             6507..7226
                     /locus_tag="VMZ53_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUS26962.1"
                     /translation="MKFSGQAPARFVDVCVQTCLASKDRGADSCCILLHCTEGPPILM
                     VDNDPNVSDADRVEHQLSDASWQHDELLRAMRERIAPVGIDGDGVHRFAFPIIGRDGW
                     FATILIGNGRPIVATVERDLSLIGTQLSVWCVAHGVAAVPAIEERLSPAEWRVAKHVA
                     RGKTNTEIAAACALSVNTVKKYLKQLFARFRIETREELTVLLQRLAPLEGVPLGVSAI
                     GDVKVTRAPDRLAWIPTWIGE"
     gene            complement(7168..7782)
                     /locus_tag="VMZ53_00585"
     CDS             complement(7168..7782)
                     /locus_tag="VMZ53_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUS26963.1"
                     /translation="MQADAIIEAATYVCDESRRRGAEHCSVQFHFGRTPTLLTIDNVA
                     ASVVDDRANRLDVPLIGPRSSIGSVLFTNPTPIAIDLERSLTMLVTELSVWCAEHGID
                     AISEHPIELPPRQYDIAQLAARGLTNVEIAQHLHISVNTVKVRLKQVFARLDASNRTE
                     LVHRLGTLSPLQNVPVGTTRARTVTITRRSTWESKPGGLVHESL"
     gene            complement(7845..8240)
                     /locus_tag="VMZ53_00590"
     CDS             complement(7845..8240)
                     /locus_tag="VMZ53_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HUS26964.1"
                     /translation="MKRVALLAALVACSSAAPPPPVPPKVADITGPRIEDSTVEGYVT
                     LVDFWSESCAACFVVADKIAPQIAGNDRVIVRKVDVGDGFTPIAQAYKISALPHWKVY
                     DTKRRLRFILVGPDCLRAPDLANQLLSER"
     gene            complement(8237..9808)
                     /locus_tag="VMZ53_00595"
     CDS             complement(8237..9808)
                     /locus_tag="VMZ53_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUS26965.1"
                     /translation="MSPKGRAVGIDLGTTNSVVAVIDRDGAPRILTTEEGSTTMPSLV
                     WFSPDGPVVGEAALEGLDQSPDLTIFGVKRLLGRRYDHPEVRKLSRTLPYELTEAPNG
                     DTWIQLAAGRAISPEEVSALILRELKRSAERFYREPVTRAVITVPAWYDAAQRQATKD
                     AAQIAGFQIMRLLSEPTAAALGHGAHRGTDRRYLVCDLGGGTFDVAAVDVDAGIFEVL
                     ATTGDAFLGGDDFDRALVEQLSRDVRQSRGIDISTDAVAIEKLRIGARRGKHELSESA
                     VATVEVAELAALPSGKPLDFKRAVRRDELELWTAPLVKRLEPPIKEAFRRIGRKREDV
                     EEVLLVGGSTRLPAVRKELARITGREPSIIPNPEEVVAIGAAIEVARLDGTIEGVLLI
                     DVAARGLMISRQGQDCEPVIAQSAVVPTREHRLIPTRYDEQVRVEFDLWEGESPDPSV
                     NRHLGRYAVVDLPEAPAGDVLVLVEVTIDTDGVIRLGATELVSGERVTLEQVYHAGLS
                     RADVSRLQKQLEQAS"
     gene            complement(9805..10938)
                     /locus_tag="VMZ53_00600"
     CDS             complement(9805..10938)
                     /locus_tag="VMZ53_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HUS26966.1"
                     /translation="MASRTLHVRCGTWDQVEAFTTRKLRRGRLLSMKVPFAAMVGNSV
                     TIGLELPNEVTIAIDGVIQKTAPVEGDASRTWIEIELIGFTDEVKARLRAQQDKRAED
                     EEPGTDPQIQSSPPARRSTSQVGDSDLPADERELFKHLTSELRRMRTAAVHDVLGVPR
                     DAGPEMVRLGWKDLIRRIHPDLIARRNAPAITHLAEELTILANRAYDRLRAALVAEGR
                     GTQVGPSLAAPAGWLVGFEDIQSDPANQPAPKKAPNRFERPPTPTPPPGPAPREPQTP
                     IGQGQDFEVRARAQLAQNDPNSAREVLAAALVVYPRSKPLRSLYYVATALAALQDGEL
                     MLATSQLETALAHHEQCIEASRLLEHLRKHGKADPAVVARLFR"
     gene            complement(10938..>12954)
                     /locus_tag="VMZ53_00605"
     CDS             complement(10938..>12954)
                     /locus_tag="VMZ53_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26967.1"
                     /translation="GGRYVTVQDLIAGAKARSKQGGKLSIPIAGLIGKSVIEALAAAH
                     KAGLIHGAVHPRSVLIDEDGGVRLTDFIAGRALTTAVAQGADSSLWRGIAGYIAPELV
                     VGEDPTPGVDVFAVGAMLFTMLSGEAPPGSLRVTPAVERLVQRALDTDSSRRYKTAND
                     MLENLLEAMEDDRWELADRGELIKEAGLSATDENIDDATEDLLASLSKGAVQVTTPRP
                     SGDIRAEMAKPKPTTGARLEALLSDLGDETGLTQIDEAPSKFRRDPISDIIRNDPRKK
                     ESIVSSARVPSLDDPDDDDAPSPAAPPQRRSPVPRAQSHPDMTQADSPSSVKRRSDVM
                     PAARPNSMDEAAALAAIGDLDEPARRMSTAAEQAESAAKKLEEAAARAERATKRAESE
                     PPLRPTPRPARPLVDPIPDMDVPTPKLRSPVKSAIGGLLLLGMLGGAAYVVYNKYNED
                     EARASQQQKDREKQQAEKDALEAKLRKAQPDTGSLEITSEGAGIWLRLGRTPLDTPIH
                     LSAGQPHDMVLLHAGFDPTEAQVNGTHWKGAKESLKAKIDVKLKPSKTKEAPEIPLQP
                     TTPVLGSTGVVGAGVVHVESTPADAEVWLFIGANHAQFNELWAGRDYEVAVVKPGFKT
                     KHVEFKAEEWRDGGDPNMPLDVAKKKALLSKSVELEPDPDAKVKKGK"
BASE COUNT         2083 a         4309 c         4317 g         2245 t
ORIGIN      
        1 gcgtatcgga tcgtctccgg cgctagatcg acgtagccgt cggccgtgac gccgcccgcg
       61 ttgatcccgc ggaggaccgc gtcggctgca aattgctttg cgagcgctcg cgccttcgga
      121 agcacctgga gcggatcgag gcgatggacg ttgtagctct cgtgcgcgaa cgcccggcgg
      181 tgcttcgggt catcccagat gagcacaccg atctgcttac tgtccgcttc gctcgcgtcg
      241 atgcgagtcg tggcatcgct gcgggtgatc gacgcgtcgc gtgcgtcgcg cttcctcgcg
      301 tcgggtggcg cggcgtctgg tggctcatcg atgaccgcaa cgacgtaggc gtcctcgggg
      361 acctcgcgga gcgcggcgtc tgaaggctcg cgctcgcgct cgaccacgac ggctgcatcg
      421 ggcgggcgcg cggcaggttt cgtacccgct tgcaacgaga ccacgacgag cgcagccgcg
      481 acggccacgc ttgcgaacgc cgcgacgacg acgagacgcg atcgcgagcg cggtggcctg
      541 tctccgacgg ttgaagcttc cggcgacggc gatggccgtg caccgcgccg ggcggccacc
      601 gttcgcgctt ccatgaggcg cggtgtcggc acgccacgct ccgccgcacg cagcgcactc
      661 gccatctcct tcgcgctctg gaaccgcgcg cgtggattct tctcgagcgc acgtgcaatg
      721 acatcgtcga acacgcccgg cagatccggc gaccactcgc gcgccgacgg tgccggcttc
      781 tcgagatgcg cgcgcatgag ctcgaagtcc gtcgctcctt cgaacggacg gcggcctgtc
      841 accgcttcga acagcacgac gcccatcgcg tacacatcgg tccgcccgtc gatgtcgccg
      901 ccgctgatct gttcgggtga catgtactcg ggcgtgccca gcgccgcgcc ggtgcgagtt
      961 cgcggcaacg agcgaccacc gatcggcgtc agcatcttcg cgattccgaa atcgaggatc
     1021 ttcgcgtgcc cgccgcgcgt gacgaggatg ttctccggct tcagatcgcg atggatgatg
     1081 ccggccgcgt gcgcggccgc gagcgcatcg agcacctcgc cgatcagctg gacgatcgtg
     1141 gcgagccctg caccatcggc aacggcctcg cgaagcgtct tgccgtcgat tagctccatg
     1201 acgatcgcag ggcgtcgatc gggcagcgtc accacgtcga tcacgttgac gatgccttcg
     1261 tgccggatcg cgttcgcggc gcgagcttcc gcgaagaacc gcttcgccat ctccggatcg
     1321 tcggcgtacg cctcgaagat caccttcacc gcgacgcgcg tgccgctcgc ctcgttcacc
     1381 gcctcgtaga cgcagcccat cccgcccttg ccgagcacgc caacgagccg gtaccgcccg
     1441 atgtgcgtcc cgacgagcgc gttcgccgcc tccgacattc gcacaagcgt agcgcgttcg
     1501 gttaccgcaa accgtggcgc ccgagcatgc gatagagata cgcgcgattg acgccggctg
     1561 agcgcgccgc ggacgcgacg ttgccgccgt ggcgctcgag cagcttcgcg agatacgtcc
     1621 gctcgaattc atcgagggct tggcgccgcg cgatctcgta cgaggcgtcg gcatcgacct
     1681 tcaccgcggg atgcgcgttg ctcggcgtat tcggcaggcg gcgctcgccg aacaccgcgc
     1741 actgctcgat gtggttgcgc agctcgcgga cgttgccagg ccacggcgcc gccgcgagcg
     1801 cgctcaagaa ctccggctgc gtcagctcgg tgaccgtgct atcgctcgcg ccgatcttcg
     1861 cgagcagatg gcgaacgagc gggggtatgt caccgatgcg ctcgcgcagc ggcggcagcg
     1921 agacgcgcac gacggcgagc cgatagtaga ggtccgcgcg aaacgtgccg cgattgacct
     1981 cggtgcgcag gtcgcgattc gtggctgaca cgatgcgcac atcgcagcgc accgccgtcg
     2041 acgagccgac gcggcggatc tcgcgcgact cgagcgcgcg caaaaacttt ggctgcagct
     2101 ccgatgggag ctcgccgatc tcgtcgagga acagcgttcc gccagaggct tgctcgaacg
     2161 caccgatgcg tcgctgcgtc gcgcccgtga acgcgcccgc ctcgtgaccg aacagctcgg
     2221 actcgagcag gttcgccgga atcgcgctgc aatcgacgac gatgaacgga ccacgcgctc
     2281 gagcgctcgc ttcgtggatt gcatccgcga cgccttcttt gccggtgccc gtctcgcctt
     2341 cgatgagcac cgtcgcttcg gtcgccgcga tcttctcgag ctgcgagaac acctcgcgca
     2401 tgaccgcgct ctcgccgatc agcgcgccga acgcaggtcg ctcggacgcg gcgagctcgg
     2461 tcgcttgcga cgcgacgtcg atgcgcaccg cggactcgcc gagcacgagc acggttcctc
     2521 cggagaccat ggcgtcggta agcgcgatat ctccggcgag cgtgccgttg cgactgccga
     2581 ggtcgcggac gcgcaccgcg ctgccggcaa tcgcgagctc gcaatggaat cgcgacaccg
     2641 tcggatcgtc gatcacgaga tcgttcgacg gatgggagcc gatcgcgcac cgctcgctga
     2701 gctgcttcca gactttcccc ttctcgcgcc cagcgctcac catcacgcga aactgctgaa
     2761 tcgcgcgtcg gggccgttcc gtcggccgcg tcatcacggc gggaggcttg cccatacccg
     2821 tcatcatagc caatccgctg cgcctccgcg gatctccgag gagcggaacc tgcatcgcaa
     2881 tgcgtcagga ggtagacgat gacaacgctt tcttctgcag gctgggtcgt tcatgaggtt
     2941 ggtctcgcaa cgtcgatcgg cggaacgtta ttcgggcgcg ctgcgctcga gccctcactc
     3001 tctgaaatca cgagctccga ggagcgcgac cgcgtgtcgg cgagcgcctg gcagcggttc
     3061 tcgtggctga acctcgcttc gcacgcagcg ttcgcgatcc cgtggttcat cggacgctcg
     3121 atgctcagcg gcaacgaggt cacatgcgag gcacgacgcc tcactctggt caaagatgtt
     3181 ctcgtcggtg cgagtctgat aaccggcgtg tcgagcattc ttctcgggcg ctggctcggc
     3241 aagcgtgccg acgctggcca gggtcccgcc gaggcgcgcg atcgcatgtc gccggaaggt
     3301 gaagccgaag gcgaagccgc caagacccag aagctgcagc gcgcggtggg attgctcggc
     3361 aacgcgaaca tcgcggccaa tatcggcgtc ctcggtgtga ctacgctgct cgcgatgcaa
     3421 ggcaacaagt cccagcggtt cagcttcttc agccgcttcc ttccgtgatc acggcgagct
     3481 gatcgtcacc gtcgagtcgg tcgtgtcgca gctgtcgtcc ggtcccgctg cgacatgaag
     3541 ctcgacgaac gtgccgacgg ccagctcgac gtcgacgacg tcatcgaacc cggtgccgtt
     3601 gtcgaacgcg atcgcgcggt gccagcgcga cgtgccgtcg accacgagcg tcgcgccgac
     3661 gccgtcaccg cagctggtgt cgcccttcga cacttggact ctcaccttgg ccgggccttg
     3721 cgcgttgctc gtccagcgcc ggacggtgac gttgctcggc cctgcgtcgg gatgagtgcc
     3781 ccaccacgcg agatacagaa acgggctgac gttattcggc gggacgaaca cgttcgtgtc
     3841 ccagacgccg aggacgaagt ccgtcgcagg ttcgtacgac ggatcggtct ccgcggccca
     3901 gaagccgtac gtccagccat tcgcaccctg cacgtcgttc acgaaatccg cgtcggaggc
     3961 cgcgagcaga cacgacgctt gcggcatctc gggcacgcac gcgccagcgc ggcagctcga
     4021 ggtcgtcgtg cagacgttgc ggtcatcgca cgccgcgccg ttcggcagcg tgctgcaatc
     4081 gacgaccgcc ggggcgtccg gcaccgccaa accgctgtcg gcgacgctcg gatcgaagct
     4141 gagacggccg cacgcgccga gcagcaggag gagtactatc cgcacgtgcg ttcccgcatc
     4201 ataacgctgc tgcttgtcgt cgtcatcggc gtggtcggtt ggggcgtcta ttcgaacgtc
     4261 ctctccgacg acaccgccgt ccgccagctc gcggaggcac gtgcgcgtgc ggtcgcgggc
     4321 tgcggcgcca agtgcgtcgt caagaagctg gaaggcagcc gcggcctctt gaacgagcag
     4381 ctccaattcg aattcaccga gggctcgaag atcctcgtcg agtgcaagcg cgcgtacatc
     4441 gctttcggcg catacacctg tcgctgacgc ggtttaacag cgctgcgtgg tggggctcgc
     4501 gatgagaacc acggtctcat ggcaactcga gctgtgcaca ccgtaggtat cgcgtgcgca
     4561 accgtccgct cgtcgagaac gcgcggttcg tgcaacgcgc tgcacgacgc gatcgaggct
     4621 ctgcacgcga gggaacgtga cgactcggtg agtagatcgg cgcgccgttt gctgatggtc
     4681 cgccggcatg atgatgaagc gcgcgttcgc attgggtttg gtagcgatcc ccgcctgcac
     4741 cgccggcagc tcgggcaccg ctagcgtcga ccagagcatc atcggcggaa ccaccacgca
     4801 gactgccgac tggaagtcgg tcgtcgccct cgaagacaat cccggcgact ggttctgcac
     4861 cggcacgctg atcaccgacc agtgggtcct caccgccgcc cactgcatgg aaggcgagac
     4921 tcccgcgaag ctccacgtcc gcttcgacac gaacgacgcg aacaacggca cgaacggtca
     4981 cgccgtcgcc gtcgccgaga ttcactcgaa ccccgcattc aacttcgacg cgtgggacaa
     5041 cgacgtcgcg ctcatcaagc tcgcagcccc cgtcacagat cgcacgccga cgccgatcaa
     5101 ccgcgatgcg atcgcgttcg gctcgcaagt cgtcgatgtc ggctacggcg tctcggacaa
     5161 caacgacggc ggcggcggaa tcctgcgcaa ggtcagcaag gtcacggcgg actgcgcggg
     5221 tgccaacgac accggcatcg tcaacgacaa cctcgtctgc atggatgcaa gcgatggtcg
     5281 cggctcgtgc ttcggcgaca gcggcggtcc gacgttcgtc acggtcggcg gcgcgctgca
     5341 agttgcgggc gtcacgtcgg gcggtaccgg tgacgcatgc ggcgcaggct gggacctcta
     5401 cacgtcagtc cacggcgagc tcgcgttcat cgacatgatc atgagcgcgc cgtccgatcc
     5461 gggcggcggt gatggctccg gctccggcga aggttccgga tcgggctccg gctccggcat
     5521 ggacacgaac gatccgagca atcccggcaa cggagaagat ggcggctgca gcgcggccgg
     5581 cggcggctcg tcgagcgcgc tcctcgtatt cgcactcgcc gcggtgattc gccgtcgtcg
     5641 acgcgctacg gacgcgaagc cggcggagta gccggccagc cctcctcggg cgcgggcagt
     5701 cgggtcacgc gatacgtcga ccacgtcttc agcacgagca tcgcaccgca cacgatggtg
     5761 ccggcagtcg ctgacttgct cacgacgagc acgccggtga cagccgccgt gttcaccgcc
     5821 tttgcgaacg gcgaccagtt ccaccgccag atttcccgat cgatctcgta gaagtagttc
     5881 gggctcttga ggcgccagcg catgaactga ttcgacagaa agtggtcgat gaacatgaac
     5941 tggacaagga acagacacac cggcacgagc agctgcggat agaacgtcgc gtagttgaaa
     6001 tagaagaacg cgacgagcac gcgatcgacg aggatgtcga gttgcgcgcc gatgcgcgtc
     6061 tcctgatcga gccagcgagc aagcgcgccg tcggcgacat cgagaatcca atacgcgacg
     6121 agcccgacga ggttcgcggt ttcatcggcg cacgcgcgcg cgtaaccgaa gatcgcgacg
     6181 caggcgacga ggcgaaatag cgtcacggca ttggcccacg tcgtccagcg ctcggtcact
     6241 ggcctccgtg catgctgtcg aacacgcgtt cgagcagtcg acgtacccac cacgcgccag
     6301 cgatcacgac gactccaccc acgatccaag gccacacgcg gccacactga agaggcaggc
     6361 gcgacccggc aagtacccac gtgggtatgg agatcgcggc tcgcgtagag ccgtacccat
     6421 gtgggtatgc attctgcagg cttacctcaa aatgtacccg ggcgggtacg ggcgcgcggc
     6481 gacgcgcttc tttatatccg aatgcagtga aattctccgg gcaggcccca gctcggttcg
     6541 tcgacgtctg cgtgcaaacg tgcctggcgt cgaaggaccg gggcgcggac tcgtgctgca
     6601 ttttgctgca ttgcacggag gggccgccga tcttgatggt cgataacgat cccaacgtct
     6661 cggacgccga tcgcgtcgag caccagctat cggatgcgag ctggcaacac gacgagctgt
     6721 tgcgcgcgat gcgagagcgc attgcacccg tcggcatcga cggcgatggc gtgcatcggt
     6781 tcgcatttcc gatcatcgga cgcgacggct ggttcgcgac gatcttgatc ggaaacggtc
     6841 gtccgatcgt agcaaccgtc gagcgcgacc tgtcgctcat cggcacccag ctgtctgttt
     6901 ggtgcgtcgc gcatggagtc gctgcggtgc ccgcgatcga agagcgcctg tcacccgcag
     6961 agtggcgagt cgcgaagcat gtcgcgcgcg gcaagaccaa cacggagatc gcggccgctt
     7021 gtgcgctttc ggtgaacacg gtgaagaagt acttgaagca gctgttcgca cggtttcgga
     7081 tcgagacgcg cgaggagctg accgtgctgc tgcagcggct cgctccgctc gagggcgtac
     7141 cgctcggtgt gtccgcgatt ggagacgtca aagtgactcg tgcaccagac cgcctggctt
     7201 ggattcccac gtggatcggc gagtgatcgt tacggtgcgc gcgcgcgtgg tgcctaccgg
     7261 aacgttctga agcggcgata acgttccgag ccggtgaacg agctcggtgc gattgctcgc
     7321 atcgaggcgc gcgaacacct gcttgagtcg cacctttacc gtgttgaccg agatgtgcag
     7381 gtgttgcgcg atctcgacgt tcgtgagccc acgagcagcg agctgagcga tgtcgtactg
     7441 gcgaggaggc agctcgatcg gatgctcgga gatcgcatcg atgccgtgct ccgcgcacca
     7501 cacggagagc tcggtgacga gcatcgtgag gctgcgttcg aggtcgatcg cgatcggagt
     7561 cggattcgtg aagaggacgc tgccgatcga gctgcgcggt ccgattagcg gcacgtcgag
     7621 gcgattcgcg cgatcgtcga cgacagatgc cgcgacgttg tcgatcgtca ggagcgtcgg
     7681 cgtgcgacca aaatggaatt ggacgctgca gtgctctgcg ccgcggcgac gcgattcatc
     7741 gcacacgtac gttgccgctt cgattatcgc gtcagcttgc atcgcttcgt tcaagctaga
     7801 cgacgtcacg cgtttatggc gccgcgttca cgcgttgttc acgcctagcg ttctgaaagc
     7861 agctggttcg cgaggtcggg tgcgcgcaag cagtcaggtc cgaccaagat gaagcgcaag
     7921 cgacgcttcg tgtcgtagac cttccaatgc ggcagcgcgc tgatcttgta cgcctgcgcg
     7981 atcggcgtga agccgtcgcc gacatcgacc ttgcgtacga tgacgcgatc gttgccggcg
     8041 atctgcggtg cgatcttatc ggcgacgacg aagcacgcgg cgcacgactc gctccagaaa
     8101 tcgacgagcg tgacgtaccc ctcgacggtc gagtcttcga tgcgcgggcc cgtgatgtca
     8161 gcgaccttcg gaggtaccgg cggtggcgga gctgcgctgc tgcatgcgac gagggcagcg
     8221 agcagcgcga cgcgcttcac gacgcctgct cgagctgctt ctgcagacgc gagacgtcgg
     8281 cgcgcgagag acctgcgtga tagacctgct cgagcgtcac acgttcgccc gagacgagct
     8341 cggttgcgcc gagccggatg acaccgtcgg tatcgatcgt cacttcgacg agcacgagca
     8401 cgtcgcccgc gggcgcttcc ggaagatcga cgacggcgta gcggccgagg tgacggttga
     8461 cggatggatc gggcgactcg ccttcccaca gatcgaactc gacgcgtact tgctcgtcgt
     8521 agcgcgtagg gatgaggcga tgctcgcgcg tcggcacgac cgctgactgc gcgatcacgg
     8581 gctcgcagtc ttggccttgg cgcgagatca tgaggccgcg cgcggcgacg tcgatgagca
     8641 ggacgccttc gatcgtccca tcgaggcgag cgacttcgat cgcggcaccg atcgccacga
     8701 cctcttcagg attcgggatg atgctcggct cgcgccccgt gatgcgggcg agctctttgc
     8761 gcacggccgg caggcgcgtg gagccgccga cgagaagaac ctcctcgacg tcttctcgct
     8821 tgcgaccgat gcggcggaac gcttctttga tcggcggctc gaggcgcttg acgagcggcg
     8881 cggtccacag ctctagctcg tcgcggcgaa ccgcgcgctt gaagtcgagg ggtttgcccg
     8941 atggaagcgc cgcgagctcg gcaacttcga ccgtcgcgac cgcgctctcg gagagctcgt
     9001 gctttccgcg acgtgctccg atgcgcagct tctcgatcgc gacagcatcg gtcgagatgt
     9061 cgatgccgcg cgactggcga acatctcgcg agagttgctc gacgagcgcg cgatcgaagt
     9121 cgtcaccgcc gaggaacgcg tcgcccgtcg tcgcgagcac ctcgaagatg ccggcgtcga
     9181 catcgaccgc cgcgacgtcg aacgtgccac cgccgagatc gcacacgaga tagcgacgat
     9241 ccgtgccgcg atgcgcgcca tggccgagcg ctgctgcggt cggctcgctc aagaggcgca
     9301 tgatctggaa gcccgcgatc tgtgcagcgt ccttcgtcgc ttggcgttgc gcggcgtcgt
     9361 accaagcagg caccgtgatc accgcgcgcg tgacgggctc gcgatagaag cgttccgcac
     9421 tgcgcttgag ctcgcgcagg atcaacgcgc tgacttcctc gggcgagatc gcgcgacccg
     9481 cggcgagctg gatccaggta tcgccgttcg gcgcttccgt cagctcgtac ggcagcgtgc
     9541 gcgacagctt gcggacctcg gggtgatcgt agcgccggcc gagcaagcgc ttcacgccga
     9601 agatcgtgag gtcggggctc tggtcgaggc cttcgagcgc cgcttcgccg acgacaggac
     9661 catcgggcga gaaccacacg agcgacggca tcgtcgtcga gccttcttcc gtcgtgagaa
     9721 tgcgcggcgc gccatcgcga tcgatcaccg cgacgacgct gttcgttgtt ccgagatcga
     9781 tgccgactgc gcggcccttc gggctcatcg gaacaacctc gcgacgacag cggggtcggc
     9841 cttgccgtgc ttgcgcaggt gctcgagcag gcgcgacgcc tcgatgcact gctcgtgatg
     9901 cgcgagtgcc gtctcgagct gcgaggtcgc cagcatgagc tcgccatcct gcagcgcggc
     9961 gagcgcggtg gcgacgtagt agagcgaccg cagcggcttg ctgcgcggat agacgacaag
    10021 cgcagcggca agaacctcgc gcgcgctatt cggatcgttc tgcgcgagct gcgcacgcgc
    10081 gcggacttcg aagtcctggc cctgaccgat cggcgtctgc ggctcgcgtg gcgctggacc
    10141 cggtggtggc gtcggcgtcg gcggccgctc gaaccggttc ggtgctttct ttggcgcggg
    10201 ttgattcgcc ggatcgctct ggatgtcctc gaagccgacg agccatcccg cgggtgccgc
    10261 gagcgacggc ccgacttgcg tgcccctgcc ctctgcgacg agcgccgcgc gcagacgatc
    10321 gtaggcgcgg ttcgcgagga ttgtcagctc ctcggcgagg tgcgtaatcg caggtgcatt
    10381 gcgacgcgcg atgagatcgg ggtggatgcg gcgaatcaga tctttccagc cgagtcgcac
    10441 catctcggga cctgcatcgc gagggacacc gaggacgtcg tgaacagccg cggtccgcat
    10501 gcggcgaagc tcgctcgtga ggtgcttgaa cagctcgcgc tcgtcggcgg gaaggtccga
    10561 gtcgccgacc tgcgaggtcg accggcgcgc cggtggcgac gactggatct gcggatccgt
    10621 accgggttct tcgtcttcgg cgcgcttgtc ctgctgcgcc cggaggcgcg ccttcacctc
    10681 gtcggtgaat ccgatcagct cgatctcgat ccacgtgcgg ctcgcgtcgc cttcgaccgg
    10741 cgcggtcttc tggatgacgc catcgatcgc gatcgtgacc tcgttaggaa gctcgagccc
    10801 gatcgtcacc gagttgccga ccatcgcagc aaacgggacc ttcatggaca gcaggcggcc
    10861 gcgacgcagc ttgcgcgtcg tgaaggcttc gacctggtcc cacgtcccgc aacgcacgtg
    10921 aagcgtgcgc gaagccatta cttgcccttc ttcaccttcg cgtcggggtc cggttcgagc
    10981 tcgaccgact tcgagagcag tgccttcttc ttcgcgacat cgagcggcat gttcggatca
    11041 ccgccgtcgc gccactcctc ggccttgaac tcgacgtgct tcgtcttgaa gccaggcttc
    11101 acgacggcga cttcgtaatc acggccggcc cacagctcgt tgaactgcgc gtggttcgca
    11161 ccgatgaaca gccacacttc ggcatcggca ggcgtcgact cgacgtgcac cacgcccgcg
    11221 ccgaccacgc ctgtcgaccc gagcactggc gtcgtcggtt gcagcgggat ctccggcgcc
    11281 tctttcgtct tcgacggctt gagcttcacg tcgatcttcg ccttgagcga ttccttcgcg
    11341 cccttccagt gcgtgccgtt gacctgcgcc tctgtcggat cgaagccggc gtgcagcagc
    11401 accatgtcgt gcggctgtcc ggcggagaga tggatcggcg tgtcgagcgg cgtgcggccg
    11461 agacgcagcc agatgccggc gccttccgac gtgatctcga gcgagccggt gtcgggttgc
    11521 gccttacgca gctttgcttc gagcgcgtcc ttctcggctt gctgcttctc gcggtccttc
    11581 tgttgctgcg acgcgcgcgc ttcgtcctcg ttgtacttgt tgtaaacgac gtacgcggcg
    11641 ccgccgagca tgccgaggag caacaggccg ccgatcgcgc tcttcaccgg agagcgaagc
    11701 ttcggagtgg ggacgtccat gtccggaatc ggatcgacga gcggtcgcgc aggacgcggc
    11761 gtcggtcgca gcggtggctc gctctcggcc cgcttcgtcg cacgctcggc acgcgcggca
    11821 gcttcctcga gcttctttgc ggccgactcg gcttgctccg ccgctgtcga catccggcgc
    11881 gcgggctcgt cgagatcgcc gatcgccgcg agcgctgccg cttcatccat gctgttcggt
    11941 cgcgcggccg gcatgacgtc ggagcggcgc ttcacgctgc tcgggctgtc cgcctgcgtc
    12001 atatcgggat ggctctgcgc acgcggcacc gggctgcggc gctgcggcgg cgcggcggga
    12061 ctcggcgcat cgtcgtcgtc gggatcatcg agcgacggca cacgcgccga agacacgatc
    12121 gattcctttt tgcgcggatc gttgcggatg atgtcgctga tcggatcgcg ccggaacttc
    12181 gacggtgcct cgtcgatctg ggtcaggccc gtctcgtcgc cgagatccga gagcagcgct
    12241 tcgaggcgcg cgccggtcgt cggcttgggc tttgccatct cggcgcggat gtcgccgctt
    12301 ggccgcggcg tcgtgacttg caccgcgcct ttcgagagcg atgcgagcag atcctcggtg
    12361 gcgtcgtcga tgttctcgtc ggtggccgac aggcccgctt ctttgatcag ctcgccgcgg
    12421 tctgcgagct cccagcgatc gtcttccatc gcttcgagca agttctcgag catgtcgttc
    12481 gcggtcttgt agcgacgtga cgaatcggta tcgagcgcgc gctggacgag cctctcgacg
    12541 gcaggcgtga cgcgcagcga tccaggcggc gcttcgcccg agagcatcgt gaacagcatc
    12601 gcgccgacgg cgaatacgtc gacgccgggc gtcgggtcct cgccgacgac gagctccggc
    12661 gcgatgtacc cggcgatgcc gcgccatagc gatgaatcgg cgccctgcgc gaccgcggtc
    12721 gtgagcgcgc ggccggcgat gaaatcggtc aggcgcacgc cgccgtcctc gtcgatcagc
    12781 acgctgcgcg ggtgcaccgc gccatggatg agacccgctt tgtgcgcggc cgcgagcgcc
    12841 tcgatgacgc tcttgccgat gaggccagcg atcggaatcg acagcttacc gccctgcttg
    12901 ctccgcgcct tcgcgcccgc gatgagatcc tgcaccgtga cgtagcgacc gccg
//
LOCUS       DATLBZ010000017         5889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5970052, whole genome shotgun sequence.
ACCESSION   DATLBZ010000017 DATLBZ010000000
VERSION     DATLBZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5970052"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1025)
                     /locus_tag="VMZ53_00610"
     CDS             complement(51..1025)
                     /locus_tag="VMZ53_00610"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HUS26968.1"
                     /translation="MPTMNIIEAVRSALQLQMRADQRVVVLGEDVGKFGGVFRATSGL
                     YDEFGSDRVIDTPLAEAGIIGTAIGMALYGLRPVPEIQFGDFIFPAFDQIVNELAKFR
                     YRSGGQYPCPVVIRTPVGGGIRGGHYHSQSPEALFIHTPGLKVVAPSNPYDAKGLLLA
                     CMRQNDPVLFMEPKRIYRASKGEVPEGEYTLELGKAAVTREGTQITLLAWSGMVSIAE
                     EAHKKAAEAGISVEVIDLRTLMPFDIDTILESVKKTGRCVIVHEAPRTCGFGAELIAS
                     IQERAMEHLEAPIARVTGFDTPFPYSLEHEYLPNPDRVVGAIRQTLEW"
     gene            complement(1029..2141)
                     /locus_tag="VMZ53_00615"
     CDS             complement(1029..2141)
                     /locus_tag="VMZ53_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HUS26969.1"
                     /translation="MVAVPAQDDLRRVLDDDGKLLPGVKAPTIPDATLQRIFDCMMLV
                     RIMDDRMMRLQRQGRLGFYMKSIGEEASHFAVSPLRDSDWIFPSYREQGAWFWRGYTV
                     QQFIDQMFGNVDDPIKGRQMPVHHSASWLNLVSISSPVGTQIPQAVGAAYAAKVMGKD
                     DVSMVYFGEGTSSTGEFHVGLNFAGVWKAPCVFICRNNGWAISVPREKQTAAKTFASK
                     AAGYGMPGVRVDGNDMLAMWQVASEAIERARAGEGPTLIEALTYRVQGHSSSDDPSVY
                     RDPKEPEIWEKKDPLNRLRGYMRTRGLWNEGWEKEISERYNQQITDALAAADTKHAPA
                     IETMFDDVYEDMPWNLREQREWLMQQARTKNPHHHG"
     gene            2229..2861
                     /locus_tag="VMZ53_00620"
     CDS             2229..2861
                     /locus_tag="VMZ53_00620"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HUS26970.1"
                     /translation="MIWILLVVLTIVGGVAAVGVASRERKSLGSGSGGDPKALPAGGG
                     DKLLERTVRDVRVDDVLTIDNKDFIVEGLVSYDEDGHRWSAARCADGTDVRWVFVGIE
                     RTGSSATRLMALDESTPMTGYPPEALVIGETRYALDKRGTATCTLRGDVGPLGGLTKD
                     RPEGHTERCRWWLYNAPGDDTVIVEQWGSDYRVLRGKKVSESTIELMPGS"
     gene            complement(2870..3646)
                     /locus_tag="VMZ53_00625"
     CDS             complement(2870..3646)
                     /locus_tag="VMZ53_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUS26971.1"
                     /translation="MATGSAAGSGAGSGSAALVIPPYTPAADTSEPIKAAIAATDRSP
                     EDRALDAGRKPGEVFTFFKIAPGQKIGELFSGGGYSTELMARIVGDTGVIYAQNTKEI
                     NDKFARKPFELRMAKAVMKVVKPVEAPADAPFPADAKDLDAVVCVLNYHDFVWQKVDR
                     AKLNKAVFDALKPGGIYAIVDHSAAATSGDRDAETLHRIDEELVTKEITAAGFKLDAE
                     SAVLRNPEDKRDWNSSPKAAAEKRGTSDRFTLRFVKPAKK"
     gene            complement(3818..4930)
                     /locus_tag="VMZ53_00630"
     CDS             complement(3818..4930)
                     /locus_tag="VMZ53_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS26972.1"
                     /translation="MLEFVIEAMADAVMVIDAEGRITLANAGAAHLSGYTRDQLRGMP
                     IAKLLSDDSSGLRTIVRRRIEGGAVLHREDSWLITTAGERIPVGVTASPVVDDKDRLH
                     GIVLVAHDMREVRAEIARRRAAEEELRRVNTSIEDKLHETRQQLLLAERRATLGTLAG
                     GVGHELRNIAQIQIAAVDELAGALRAHEDVTALARQILPELERVGDHITTHGNRLMQL
                     AHPGSDQSEQLDFGEIARDVAAMLRLAGKLGRVELNVDAGAAPVYVTANRTRVEQVLV
                     NLIINAIDAASPTGAVAVTAKKARGRIYCSVADTGPGIPAEHREKIFEPFFSTKGDKG
                     TGLGLPVVKEIITTYGGTLAFVTGPEGTTFTFDLPA"
     gene            4971..>5889
                     /locus_tag="VMZ53_00635"
     CDS             4971..>5889
                     /locus_tag="VMZ53_00635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS26973.1"
                     /translation="MARCSTCHRRLTQGRACVTHGGVVGDVAAVAAESFAWPTPVGAL
                     IGSGGFASVWELAPDRVIKIAHASHELSRARVVREAEALRAIGAPAVPRIHDSGVLAD
                     GRAWIVMQRVQGATLADVCAEGGLRTRENVAVGLSTLDALEAVHAARFVHRDLKPDNL
                     VRRADGSIVILDLGLARKLPTDPDDPTRANVQVGSLEYMPPEQIADSAAVDERSDIYA
                     FGCVLYELCAGRPPFVGDAQVLERSHTALRPPRLNAIVTVPAAVDALVNDCLAKDPAR
                     RPASVAEVRERLAVSRDERTPPQMSRSMSV"
BASE COUNT          934 a         1902 c         1970 g         1083 t
ORIGIN      
        1 ctcgacgacg ccctcgccga tatcgggcat gtagaagtcg aatgccatgg ctaccactcc
       61 agggtctgac ggatcgcgcc gacgacgcga tcggggttgg ggagatactc gtgctcgagg
      121 ctgtaaggga acggcgtgtc aaagccggtg acgcgtgcga tcggcgcctc gaggtgctcc
      181 atcgcgcgct cttggatcga agcgatcagc tccgctccaa agccgcaggt gcgcggcgcc
      241 tcgtggacga tcacgcagcg gcccgtcttc ttcacgctct cgaggatcgt gtcgatatcg
      301 aacggcatca gcgtgcggag gtcgatcacc tcgacggaga ttccggcctc ggccgccttc
      361 ttgtgcgcct cttctgcgat cgacaccatg ccgctccaag cgagcaacgt gatctgcgtg
      421 ccttcgcgcg tgacggcggc cttgccgagc tcgagcgtgt actcgccttc cggcacttcg
      481 cccttgctcg cgcgatagat gcgcttgggc tccatgaaca gcaccggatc gttctggcgc
      541 atgcacgcga ggagcaggcc cttcgcgtcg tacggattgc tcggcgcgac gaccttgagg
      601 cccggcgtat ggatgaagag cgcctcgggc gattgcgagt gatagtgacc gccgcggatg
      661 ccgccaccga cgggcgtgcg gatcacgacg gggcagggat actggccgcc cgagcgatac
      721 cggaacttcg ccagctcgtt cacgatctga tcgaacgccg ggaagatgaa gtcgccgaac
      781 tggatctcgg ggaccggtcg caggccgtag agcgccatgc cgatcgcggt gccgatgatg
      841 ccggcctcgg cgagcggcgt gtcgatcacg cggtcggagc cgaactcgtc gtagagaccg
      901 ctcgtcgcgc ggaacacgcc gccgaacttg ccgacgtcct caccgaggac aacgacgcgc
      961 tggtccgcgc gcatctgcaa ctgcagcgcg cttcgaaccg cttcgatgat gttcatggtg
     1021 ggcatgggct atccgtgatg gtgcgggttc ttggtgcgcg cctgctgcat cagccactcg
     1081 cgttgctcgc gcaggttcca gggcatgtct tcgtagacgt cgtcgaacat cgtctcgatc
     1141 gccggcgcgt gcttcgtgtc cgcagcggcg agcgcgtcgg tgatctgctg gttgtagcgt
     1201 tcgctgatct ctttctccca gccctcgttc cacaggccgc gcgtgcgcat gtagccgcgc
     1261 aggcgattca gcggatcctt cttctcccag atctctggct ctttcggatc gcgatagacg
     1321 ctcggatcat cggagctcga gtggccttgc acgcgatacg tcagcgcttc gatcagcgtg
     1381 gggccttcac ccgcgcgtgc gcgctcgatc gcttcgctcg cgacctgcca catggcgagc
     1441 atgtcgttgc cgtcgacgcg aacgccgggc atgccgtagc cagccgcttt gctcgcgaac
     1501 gtctttgccg ccgtctgctt ctcgcgcggc acgctgatcg cccagccgtt gttgcggcag
     1561 atgaacacgc acggtgcctt ccacacgccc gcgaagttga ggccgacgtg gaactcgccc
     1621 gtcgaggacg tgccctcgcc gaagtacacc atcgacacgt cgtccttgcc catgaccttc
     1681 gccgcgtacg ccgcgccgac cgcttgcgga atctgcgtac cgaccggcga gctgatcgag
     1741 acgaggttga gccagctcgc cgagtggtgc accggcatct gccgaccttt gatcggatcg
     1801 tcgacgttgc cgaacatctg atcgatgaac tgctgcacgg tgtagccgcg ccagaaccac
     1861 gcaccctgct cgcgatagct cggaaagatc cagtccgagt cgcgaagcgg cgagactgca
     1921 aagtgactcg cctcttcacc gatcgacttc atgtagaagc cgaggcgacc ctgccgctgg
     1981 agccgcatca tgcggtcatc catgatgcga accagcatca tgcagtcgaa gatgcgctgg
     2041 agcgtggcat ctggaatggt cggcgctttg acgccgggta gcagcttgcc gtcgtcgtcg
     2101 aggacgcggc gcagatcgtc ttgcgcgggg acagcgacca tgtcggcggt acgtttatct
     2161 gacccccgcc atgagtcaag gatcgctggc gtattgcgtt ggtgcactgc ggcgtagata
     2221 gtcagcgcgt gatctggatc ttgctcgtgg tgctcacgat cgtcggtggc gttgcggcgg
     2281 taggcgtggc ttctcgcgag cgcaagtcac tcggatcggg ctcgggcggc gaccccaaag
     2341 cgctgcctgc cggcggcggc gacaagctgt tggaacgcac cgtgcgcgat gtgcgggtgg
     2401 atgacgtcct cacgatcgac aacaaggatt tcatcgtcga aggcctcgtt tcgtatgacg
     2461 aggacggtca ccgatggtcc gcggcccgct gtgccgacgg cacggatgtc cgctgggtgt
     2521 tcgttggcat cgagcgaacg ggctcgagtg cgacgcgcct catggcgctc gacgaatcga
     2581 cgccgatgac agggtatccg cctgaagcgc tcgtgatcgg cgagacccgc tatgcgctcg
     2641 acaagcgcgg caccgcgacc tgtacgctgc gcggtgacgt cggcccactc ggtgggctca
     2701 cgaaagatcg gccggaaggc cacaccgagc gctgccgctg gtggctctac aacgcgcccg
     2761 gcgatgacac ggtgatcgtc gagcagtggg gctcggacta ccgcgtgctg cgcggcaaga
     2821 aagtcagcga gtcgacgatc gagctgatgc ccggcagctg accgagcagc tacttcttcg
     2881 ccggcttcac gaaccgcagc gtgaaacggt cgctcgtgcc gcgcttctcc gccgccgcct
     2941 tcggcgagct gttccaatcg cgcttgtcct cgggattgcg cagcaccgcg ctctccgcgt
     3001 cgagcttgaa cccggctgcg gtgatctcct tcgtcacgag ctcttcatcg atgcgatgga
     3061 gcgtctcggc gtcgcgatcg ccactggtgg ctgccgcgct gtgatcgacg atcgcgtaga
     3121 tgccgcccgg cttgagcgcg tcgaagactg ctttgttgag cttcgcgcgg tcgaccttct
     3181 gccacacgaa gtcgtgatag ttcagcacgc acacgacggc gtcgaggtcc ttcgcgtccg
     3241 cgggaaacgg cgcgtccgct ggcgcttcga cgggcttgac gaccttcatc accgccttcg
     3301 ccatgcgcag ctcgaacggc ttgcgcgcga acttgtcgtt aatctccttc gtgttctgcg
     3361 cgtagatcac gccggtgtcg ccgacgatgc gcgccatcag ctccgtcgag taaccaccgc
     3421 cggagaacag ctcgccgatc ttctggccgg gtgcgatctt gaagaacgtg aacacctcgc
     3481 cgggcttgcg acccgcatcg agcgcgcggt cttccggcga acgatccgtc gctgcgatcg
     3541 ccgctttgat cggctcgctc gtatcggctg ctggcgtgta cggcgggatg acgagcgcgg
     3601 ccgaaccact gcctgcgccg ctgcccgctg ccgaccccgt cgccaccgcg gagcccgttg
     3661 ccgaaccggt ggtcgtcgct ggcgcgtcct tcttcccaga acacgccgcg agcagaacga
     3721 aacaaagcaa gtgcgtcttt aacatgcggc ccgaaccgta ccgcgtgctt caaaaattcc
     3781 tacacgcgcg cgcgcgaaag catcggcgcc gtcgctgtta cgcggggaga tcgaacgtga
     3841 aggtcgtgcc ctcgggaccc gtgacgaacg cgagcgtccc gccataggtc gtgatgatct
     3901 ctttgacgac cggcaggccg aggccggtgc ccttgtcgcc tttcgtcgag aagaacggct
     3961 cgaagatctt ttcacggtgc tcggcgggaa tgcctgggcc ggtgtccgcg acggagcagt
     4021 agatgcggcc gcgcgctttc ttggcggtga cagcgacggc gcctgtggga gaggcggcat
     4081 cgatcgcgtt gatgatcaga ttgacgagca cttgctcgac gcgcgtgcga ttggcagtga
     4141 cgtagaccgg cgctgcgccg gcatcgacat tcagctcgac gcggccgagc ttgccggcga
     4201 gacgcagcat cgcggcgacg tcgcgcgcga tttcaccgaa gtctagttgc tccgattgat
     4261 cggagcccgg atgcgcgagc tgcatcaggc gattgccgtg cgtcgtgatg tgatcgccga
     4321 cgcgctccag ctcggggagg atctggcgag cgagcgcggt cacatcttcg tgtgcgcgga
     4381 gtgcgcctgc gagctcgtcg actgcggcga tctggatttg cgcgatgttg cgcagctcgt
     4441 ggccgactcc gccggcgagc gtgccgagcg tcgcgcggcg ttctgcgagg aggagttgct
     4501 gccgcgtctc gtgcagcttg tcttcgatcg acgtgttgac gcgacgcagc tcctcttcgg
     4561 cggcgcgacg acgtgcgatc tcggcgcgca cctcgcgcat gtcgtgggcg acgagcacga
     4621 tgccgtgcag gcggtctttg tcgtcgacga cgggcgatgc ggtgacgccg acgggaatgc
     4681 gctcgccggc cgtcgtgatg agccacgaat cttcgcgatg cagcacggca ccgccttcga
     4741 tgcgacgccg cacgatcgtg cgcaagccgg agctatcgtc gctcagcagc tttgcgatcg
     4801 gcataccgcg cagctgatcg cgcgtgtagc cggacaaatg cgcggcacca gcgttggcga
     4861 gtgtgatcct gccttctgca tcgatcacca tcaccgcgtc ggccatcgcc tcgatgacga
     4921 attcgagcac tgcgctgacg aaggtagcac gcgagcgtta ccatcgcgcc gtggctcgct
     4981 gctcgacgtg tcatcgtcgt ctcacgcaag gacgcgcgtg cgtgacgcac ggtggtgtcg
     5041 tcggagacgt cgcagccgtc gcagcagaat cattcgcgtg gccaacgcca gtgggggcgc
     5101 tgatcggttc aggcggcttt gcttcggtgt gggagcttgc accggatcgc gtgatcaaaa
     5161 tcgcgcacgc gagtcacgag ctgtcgcgag cgcgagtcgt gcgtgaggcc gaagcgctgc
     5221 gcgcgatcgg agcgcccgcg gtgccgcgca ttcacgacag tggcgtgctc gcagatggcc
     5281 gcgcgtggat cgtcatgcaa cgcgtgcagg gggcgacgct cgcggatgtc tgcgcggaag
     5341 gcgggctgcg cacgcgagag aatgtggcgg tcggcctgtc gaccctcgac gcgctggagg
     5401 ccgtgcacgc cgcacgattc gttcaccgcg atctcaagcc ggacaatctc gtgcggcgcg
     5461 cggacggcag catcgtgatc ttggacctcg gtctcgcgcg caagctgccg acggatccag
     5521 acgatccgac gcgcgcgaac gtgcaggtcg gctcgctcga gtacatgccg cccgagcaga
     5581 tcgccgattc ggcggccgtc gacgagcgca gcgatatcta cgcgtttggc tgcgtgctct
     5641 acgagctgtg cgctggaagg ccgccattcg tcggcgatgc gcaggtgctc gagcgatcgc
     5701 acaccgcgct ccggccaccc cggctcaacg ccatcgtcac ggtgcccgcc gctgtcgacg
     5761 cactcgtcaa cgattgtctc gcgaaggatc ctgcgcgcag gccggcgagc gttgccgagg
     5821 tgagagagcg ccttgcggtc tcgcgcgacg agcgaacgcc gccgcagatg tcgcgatcga
     5881 tgtcggtca
//
LOCUS       DATLBZ010000018        39993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5234173, whole genome shotgun sequence.
ACCESSION   DATLBZ010000018 DATLBZ010000000
VERSION     DATLBZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 39993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39993
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5234173"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VMZ53_00640"
     CDS             complement(<1..1603)
                     /locus_tag="VMZ53_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS26974.1"
                     /translation="MSVEPYTDVTAHVRDELRRVWLRLEYQIRLGWRKESAVAEAVGP
                     EDMGRLFAVARGAQVAGADSGAEQVLEQWLAAHEHTEARIRATIESRTRSPLVDVIRT
                     YNLSQRQWAALVFSLMPEVDPSLVQGYQYLARDNSLRGLDGRLLAQLVYDTPQSRSLM
                     ARDLSPSSPLLRYRLLELTTSGPNEGLMFRRIRAATRLVYLLDGNDLDLDPELAEVAE
                     LRGGSVSGSFPAVTVDRATSALKSNDVVLAVQGQRGLGKKTLLQLAAAHWSQRLLVVD
                     SKRLSQLHMSVQQMMVRSLVRELMLLDALPAFVDVDDASVTDQDRDAMPAFFDMFLDE
                     WEGPVAITINRERMPRIHQRPLVHLNIEVPPLPVRAAMWQTVVPSLTTQDADGLAGRF
                     AIPGGIIVAAAQAAEAGRMPGSSPPVATDLDHAVAAQLHQRISRLGKKLPTPYDLEDL
                     ILDDDTRAALVEIAAAARERRRIRDQFKLRGAQGISVLFSGHPGVGKTMSGTVLAKRL
                     GLDIYEIDLSQVVSKWLGETEKNLSD"
     gene            complement(1600..2229)
                     /locus_tag="VMZ53_00645"
     CDS             complement(1600..2229)
                     /locus_tag="VMZ53_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26975.1"
                     /translation="MRLALVVVALVACADPQKTQRKVEEPTAQKGSAMNASVTVTVVG
                     SKPGRPPVDRLLVDVQLKNDDATPRWVLIPTKLPPDSGGGVDKLEQLTVPAGGSTVAI
                     GRFLGRAGTYAVKLAPGARVTLRKLELSWWRENNEKEAAFDVRLASAVKLGDAAMETW
                     FDKDPAISGAADIDMDAAKHTRSQKSPTDDEVSLAAEQSTTAPVKLAAP"
     gene            complement(2246..3457)
                     /locus_tag="VMZ53_00650"
     CDS             complement(2246..3457)
                     /locus_tag="VMZ53_00650"
                     /inference="COORDINATES: protein motif:HMM:NF039793.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26976.1"
                     /translation="MSRETDTERRHQTPSTETTTSHAPAQESTSTQDTSSFTSHGQIP
                     GVSSGAQHHSGATASEMAFGDKKKGEPEIPATKEAMGGVVVNDMKKANVEPGSGGKND
                     LNHGIHYWYNYQRHCENAGQPELWQDKYRSGHTQASSFINPHEKGRWMDWELKKGHSA
                     SQAVKDWLAGATVAECLSAVIAMEIDALRAAIGDKKFDKMFGSADPKEDKAIPAQNRM
                     HIKAGMDGTPVANYMKSTEVAEEASQGGQFGTVPPAKLDEELIPGQWYYFYNHPKYLL
                     KHPGGAWQGENSIYMGKNEAGERLWSGLGATATEDAMVDEMVRAYNAPRNDYDHRMLK
                     EDGIESADGSFKDPLYDPKSGMFPETVTKQDILKSQPFAIGGTERKGGFLATAGMELD
                     EKKVQKTREHE"
     gene            complement(3537..5267)
                     /locus_tag="VMZ53_00655"
     CDS             complement(3537..5267)
                     /locus_tag="VMZ53_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUS26977.1"
                     /translation="MGLVTRLACMFVVVLATVAHAQPTPQQRVAVEAKPSVVRVWGAY
                     IATYELFGEKVQEAVGGTGTGWFMTPDGYVATNAHVVSDIHDGDEAAKKALLKMLYAD
                     INKAYEKEISRMSNADLQRLVDAIKLVDFEKRAYIVLPNGDKLNYEIKSFGKPGSGRD
                     AAIIKVKTENAPTLVIADSAKVTVADPIVVIGYPGVADMKVLLDEKSQLQASVTDGSI
                     SSLKRASSGEQILQISAPITHGNSGGPALDMKGNVVGLATFGNESEVQGFNFLVASST
                     LMEMVNDAKVEPKESETKKAWNKGLRHFWADEYTAAIEQFQEVETLFPQHDEAQSYII
                     QSNRAKKEGREKKKPESNGGLIAGLVVGGLVLAAIGGIVISRRGKQGPPRPPHGMPMH
                     GHPPMHSPHPPHPGMHAPPQMPPGPQMYGRVQPGHMASGMPQPGMPQPAMQPGHAPVA
                     KTMAIGGPGQSGGIAPTAFGSLSIGSLTCTRGQLMGQRFALTATGIIVGRQPGVAHVL
                     VNDSRASGKHVWIGIDNGKLVAIDQGTTNGTYVNDIMRGRITRHELRDGDVVIVSEPD
                     CLSLQVRLGS"
     gene            5315..6484
                     /locus_tag="VMZ53_00660"
     CDS             5315..6484
                     /locus_tag="VMZ53_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26978.1"
                     /translation="MGFPFAYRAKSRKGRGLFRKAPDPREIGDRFGRLLKRMQGSAVL
                     RAAWKGERYLAELEISPLVPAISLVVGDDAELTITGDATFGPAVIADVAARMAPIFEE
                     LDFVWVDEAPSIETAQAMVCAKLRETLQKSDGRVRIGVPARKFLIDAPVLTALGPRDA
                     AWRDGVSAEPSRARDAFPYWEPGPGRAALARALVAMWLEIPWREPLDREERDLFKQVD
                     EDLRAARKAKLDVPWRAWQELLRHMGVEDEDVDEKAKDATDPPIGYRRHDLEAELSGG
                     WSVRMSGAMIGHWEDDGARYWATDGDRVLEFTSLTAEEERDTDKLLAVAPEAHPVIAR
                     FAEGEQRGRAEAFEDDGVAVVVGLMARAPHVGILTCKGGERDWALATWRSLQHKD"
     gene            complement(6481..8385)
                     /locus_tag="VMZ53_00665"
     CDS             complement(6481..8385)
                     /locus_tag="VMZ53_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HUS26979.1"
                     /translation="MRWLAVLVLLAAWPSLARAFDEDPPPSRVTARLSGTTIELTARY
                     DLVADASIWSGVPISTPDDSIVTRAVATVEGTRHVLALVDTQTAQKELDGVWSENLPK
                     GNHRWFVHVSGTLGFVTLDVLSPKATTSITLELTVEVPGCFFRDRRYARVPETWLEKL
                     AGAPAIDDEQALRDVCAPDAVPNERKWISFASPTLARRPVGETRIGAFAGRLPLGHDD
                     LARVEIDLASELSRVPPDLHTAIVIDHSRSLSGDEVETERAVVASYLQHVPAQSRVQV
                     IAYAREAKPLLAAWMPAATARASVDREIRSLAPRNGSNVDVAIAEAGRWLARANGTRR
                     LIIISDDRFASAIEAKTGDDIKALLPEHTLVHVVVVSAGTLGMQMTDRTQLGGIAELT
                     EGIAVAAGVDEKEPLDATMLVRPLSIDKLTIKGRGWEDLGIGTCRDTVSEGQSCTWWG
                     QGTTIAGALTVEAWLWGHKLTKTLVPDATSARHLARELSVSSHFLDDDLRAKIDHAAF
                     AVNEAWSLFGQWGGPLGQSEGGFGFGRSGFGPGGCCSTSDFATGITGVLHPTVDVKAQ
                     VAPIVERCGAKTPLVIRLQTTREEIIYVDVNAQNESATVRSCIEEGIWDLALSIPNAP
                     WRASATLKLN"
     gene            8499..9107
                     /locus_tag="VMZ53_00670"
     CDS             8499..9107
                     /locus_tag="VMZ53_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26980.1"
                     /translation="MLWLIVIGPLVLYPMARWKANRDPHPDPQLGLKVALHYFRMIAF
                     QLLLVGALIVLWALMRKTSENRGDLYRAGFGLLLPAGLVFGAHVMLLMRTNDHVLVTV
                     RRLFFGYNLLVTGLFGVTALVLAFQALFAKGPSDNEGRFYYAALVVYVCAWAGCAFHY
                     IMMIDRSAAAGPRQNMAPPPPMAAAAGPGLPSLGGGFPPVGQ"
     gene            complement(9101..10102)
                     /locus_tag="VMZ53_00675"
     CDS             complement(9101..10102)
                     /locus_tag="VMZ53_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS26981.1"
                     /translation="MSTKVRLIAITVGVLVGLTLAHKKPWKTRNAAAAELQVGYLPVT
                     CHLTCPVTDYSTKTTTTGTRFLSQRFTEFPTVVEALKARKIDATFILAPLAMVLREQG
                     VPIHIVYLGHRDGSTVIVPKNSTAKSLRDLKGKTFARPSRFSNQYLVITKLMEDEGMK
                     PDEINFVDMAPPDMPSALAAGAIDAYFIGEPYAAKAELAGTGRVLYYAKDIWPHFVSC
                     VLVVRDELIAEHPDVVRDLVRGIAESGEWVEQHRLDAAKVAAPYYRQDQKLLEFVLTQ
                     PPDRVSYRQLTPSDEDLRSIMTYAIKAGILKRPIDLSDIVDRRFIPTTIQAARIDVH"
     gene            complement(10099..10794)
                     /locus_tag="VMZ53_00680"
     CDS             complement(10099..10794)
                     /locus_tag="VMZ53_00680"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS26982.1"
                     /translation="MSDLVLEVENVGFDYGSLSVIDKLDLRVARNELLVCVGPSGCGK
                     STLLSLLGGHLAPTRGKIKRSGQTRTIYQDGGLFPWLTVRQNIGLGTGGNMKKVDEWL
                     EITRLTEFAASFPHQLSGGMRQRVELARALAAETDILLMDEPFSALDYQTRRRMRKEL
                     VSTLAARPRSVVFVTHDIEEAVQIGDRVVVLGTRPASVRKELALPPGRSRAPAAPGVA
                     EAEQTIMEELGLS"
     gene            complement(10791..11531)
                     /locus_tag="VMZ53_00685"
     CDS             complement(10791..11531)
                     /locus_tag="VMZ53_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS26983.1"
                     /translation="MLPLLCGVVLLAIWAIARELGMFPTGTVPRPIDVLTAFGTEIRN
                     GRLLEDAISSLFRVAWGFISAVLAAVPLGLVIGRHTMTREALLPWINFFRSLSPIAWI
                     PFAIIWFGVGDPPAIFIIFIATFFQVVLATAAAAASVPKVYYRVSEELGMRGQQVLFQ
                     ITLPAIMPQLVTALRVAIGVAWMVVVVAEMIAVPSGLGYLIIDARNGLRMDLVVVGMI
                     TIGAIGISLDILFSRLARIPSLRWGFDR"
     gene            11632..12489
                     /locus_tag="VMZ53_00690"
     CDS             11632..12489
                     /locus_tag="VMZ53_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26984.1"
                     /translation="MQLAIPTMKPFSFAQTLAFLERFPPCRGEYLVEDGRLTAAVSVG
                     GHARAFTLFERAGHLYIDTDDVATVPHASHFIGAADNVAAFYEAARADKPIATLVELL
                     HGLHHVRFLTLAEIAVYSVMMQRAPITIAAALKRKFLAAFGKPIDVRGHTLRAMPDLD
                     ELATLPVDAIADAIRHRGKAERIVEVVAGVRAIGEATLREAPYGEARDALLAIRGVGP
                     FSAAAILLRGLGRMDELPWLPAFSELARKHYGRAVDHATISRRYGSHIGYWSFYLMTG
                     APRLAKLAA"
     gene            12486..13865
                     /locus_tag="VMZ53_00695"
     CDS             12486..13865
                     /locus_tag="VMZ53_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26985.1"
                     /translation="MRRVLLVVVVGACRFNFDNQSATGDAGDAPMVDVLPPADFVPNW
                     QNGSRIRAIVFRAVEGGDPHWFNWRDTQLDSDCYFGIGADGVERCLPYFASAETFYSD
                     AACTQPLIYVANNACDRHIKYADDIVGSRHHIYPINGIYVGQAYDIRTGCSPATTPGG
                     TLYNAGAEVNPTMFEPVIYHTEIVGDFERTFQGFSDGAAIEIGNLNFNIGSCYPYGGF
                     LGSSRCSQSVQFIAEPVYTDAACTQRAYLAQTSTTYFSVRAAEVCGASYSLYVTTADV
                     TQASYFRRTPSGCQMTTTPAGSVLLQGSPTTNPYPTGTNIVGPDRGRLGTLYWVGPDG
                     SAMILGTWDQQLDRPCRPFVAADGKMRCLPSATPRSLAWANASCSNTQDNLAVECFPG
                     IAPLDGPSYRSCEDTPWSVRTLAKVTDTPSIANEAACVSLSTPAYDPAVPVATLSPSM
                     FPELTFTVE"
     gene            13888..14538
                     /locus_tag="VMZ53_00700"
     CDS             13888..14538
                     /locus_tag="VMZ53_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUS26986.1"
                     /translation="MKKVAVLGSGAVGDVLSNGFLTHGYAVTRATREPAKLEAWKKAA
                     KGEAAIATYADAAKWADIIVLAVKGSVAEEVLDQCGAANLANKTIIDTTNPIGNEPPD
                     HGVIRYFTNANESLMERLQKKVPAAKFVKAFNSVGNVFMVNPKFETTPTMFYCGNDTA
                     AKAEVADVLTKFGHEPFDCGGVEAARPIESLCILWCIPGFIRNDWAHSFKMIKPAK"
     gene            14616..15986
                     /locus_tag="VMZ53_00705"
     CDS             14616..15986
                     /locus_tag="VMZ53_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26987.1"
                     /translation="MRTSISLAFLLATAAAAAPTAGCGTDSTVSGVFPAEGFTGRSLR
                     VEISGDATEWSGSPAVNFGQGVTVSSVSVASPTSLFADITIAADAAPGLRDVTITNDG
                     TFTLKQSFELVSPITVSFDGMVAQGGSPYFTVTNHDFDTPFDLTSGDTPSGFANLTLD
                     TPAGTQFVVQDSTPYQLTGFALIDADAAPGTFSLSSGVMGKETAFNLGANIDITARTP
                     TPLTDSATGTLASMGDSALYSVNVATAPSLMRIVADTSDADGQPVTAVLPNGSWADAT
                     GAALSVQATSGTLDVVVFDAGKAGGYSYTLKSKIEALASAAEATTANDTPAAALAASA
                     LPFAQTGGTLSGTNDKDVIKIVLSAPAVVHVSAHSSDDLTDTAIDILANGSGNPTVLT
                     NYVDPGPPAQPPVDLGGCFPLFGLGCGEDITSPMLAAGTYFVVISAGGSFDVADKNYT
                     ALIYLN"
     gene            17213..17389
                     /locus_tag="VMZ53_00710"
     CDS             17213..17389
                     /locus_tag="VMZ53_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26988.1"
                     /translation="MKAVIATGARRQSFRHAVSFGGAAGGRVVFGATGQEETADNEND
                     DQPNALTHPETITD"
     gene            17792..18493
                     /locus_tag="VMZ53_00715"
     CDS             17792..18493
                     /locus_tag="VMZ53_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26989.1"
                     /translation="MASADDTLGNPATGAPGVSPDNEKTGTPASDAPPTPTGQLPPAK
                     SDHDPAPPVPTPAIPGVNADVVEQAGVGGAVGYGRAGVLELGGSAGLTMAQDVRALNF
                     SPSVGWFLIDNFEISAILDVTNLKAGGESATLFSALVEPSFHLPINRSMFGFVGMGIG
                     AAYQHDLGSGMAVAPRVGMDFLVGRSGILRPSLAYEYTTHDSMAAIGPDGSANVTLVA
                     ISSALRFNIGYSSMW"
     gene            complement(18592..19185)
                     /locus_tag="VMZ53_00720"
     CDS             complement(18592..19185)
                     /locus_tag="VMZ53_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26990.1"
                     /translation="MVTRIFLTVFACTSLLGVANAYADDEVEDPGFNMLDMRFAMGAL
                     PVDGAPTLTVSLGLGVEHPVFKKTRMFGEYEWMWLFDRPTERTYMIAPPPPERHGTGH
                     RAMLGLRRELIGKGRHSFRAFIDGELGGGLAITNDNMHGMQTLPTGFIGLRAGYDVYS
                     RMDDSPSRTFEFELLFRMVAVPDGIGSLFGLGMAWGN"
     gene            complement(19179..19808)
                     /locus_tag="VMZ53_00725"
     CDS             complement(19179..19808)
                     /locus_tag="VMZ53_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26991.1"
                     /translation="MRAHLWLVAILLAVPAVAHADETIDDEDGAFVPFPKRSVGLGLV
                     IHGTRIGGESESGVGPNLELAIGRDRWQYIAEFSLSKTTRHSQSVDPSLDMGIEGKVI
                     RGDIGVRWLARQFRPDSGGGIELFLLSALGAQRFYFSDGGRLSRPELAAGFGMQVRTY
                     KRPRLAFRFDARILFTPNSNESSLVQCRGRCDMEAGASTGFITGFGFAW"
     gene            complement(19862..22309)
                     /locus_tag="VMZ53_00730"
     CDS             complement(19862..22309)
                     /locus_tag="VMZ53_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS26992.1"
                     /translation="MLRRSTSHAQAPLVCALLAACSTPTHDRPLDAGAGDALDAAIGD
                     GRDADATTADAPPDAAPDASVPDTTPPHLLSVTPANGTPTWLGAPVRFVYDEPLDAQS
                     VAALTASAQLDGSVVGASVSFEAPSTIRVTLDTSARGVGSLSLHLGGSVEDAAGNAAT
                     VDEDEAFVVAPWSNVPIDRGIARSSPKLAVGTDGSVYAAWTVGPLAARKLAVSVLDGT
                     TWRDLGMFGASNVTSSAIALDENGDVVVAYIDLGSVFVSRFSGGAWASLPSPGDARYV
                     ALAKPPSGAPMLALFGSTASVSTLVGNAWQPLGMDVAMPAPLASEPVLAASAADRAAI
                     GWIDDSGALRVYRFDGAWTAMSTLTVGTNAHLSLAARGSSIAIAWDQYAGSYGVLAAM
                     ATGSATTWTRLGRALDIDMEGDAVTPAVALDSSGAPIVAWTELVEENQRGVIARWSGS
                     SWAVVGGITWLASTTGVPLRTELALARGDVPVVATAASGTAVIARFNGPRTPSLGLAT
                     RASIDGCGFSVAAPPTYLSQTGCFSLATANHPVPHAGLVPYDIVSPLWSDGALKRRWI
                     GLPDNASMTLASNGSWSAPVGSIIVKEFDIETTPGHPATRRPVETRFLINDATSGWRG
                     FSYRWNTAGTDATLQPADTAQTVNWTLDDGTAHPHVYPSRQHCNSCHYPAMGPMLGLR
                     PEQLQRWNDYDGVIAPQLPTLAALGIGPNSSTQPLVSPHEPSETAEHRMRGYMAGNCQ
                     HCHNPQYISIKDLRYTTPLAQTKLCEVIVPGDPASSVVYQKVTSRPGMPALGTVVVDP
                     LAQQLLGSWISGMTSCP"
     gene            complement(22423..23547)
                     /locus_tag="VMZ53_00735"
     CDS             complement(22423..23547)
                     /locus_tag="VMZ53_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26993.1"
                     /translation="MRIIKWPWLSVSIVPTTLQRIDFTADQVTRERIGVTVTGIAVYR
                     IAAPLLAFRVLNFQEGGASEKLAATMREMFVGAARRLIANLALDDCLTRRKEAIAGYL
                     MDEIAPVVGGEGSPEDTTNKGWGVVIDTIEIQQVRVQSQQVFAHLQAPYRAEIATRAE
                     LAELQRERQVVEQRGESERKLAEQEADLARRKSEQEAELARRRSELERERALHQIAIA
                     EEHRRAQAAAEVASLEAERGRVEALHIQSTLQLEQERQLEAMRMELDRRRAEEKDYAA
                     QLSAAHQRRLAEIENLVAQGRVVTNLVTHGLPKIAEALAQTIGTVNYTQIGNGAGTGP
                     LEAVPAAIAQLLALAKSFGVELPGASRPVDAVSHVATERT"
     gene            23781..25358
                     /locus_tag="VMZ53_00740"
     CDS             23781..25358
                     /locus_tag="VMZ53_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS26994.1"
                     /translation="MSAPFGVEARCERASISSGKSTLWASIRIDPKGKALENDRAPLA
                     VALVLDISGSMAGDPIAHVIAASQIVSTLLDERDKLAIVTFSNHAGVRCGLTTCDADG
                     RKLIGDALRAIQTQGGTNMHAGMEAAAGILATAPAGLRKTMVVLSDGQPNVGLSSAEQ
                     LAQYTTSLRPIGVSTLGFGLHHDEHILTAISTAGSGRYAYVPDPMIARVDLARAALAH
                     GGIVAEGIQLEIRPAEGVEVLRIMPAAQLRVGGSGLKASIGDVFIDEFRVFALELALD
                     VATTFRGNLAEIRIEGRSPDGKLHQVPTALIVDVHGGPHTIIRDAQRDILFVRAMATR
                     VEARAHADRGATPAAASLLREMIDAIDKSEGFVRDDASPLAEMREQLQDEVDNYERKA
                     SQAEQGHRYKMMAAYTPTLMGASTPARKKQRNDYAPGFLVGLSHGAQNRKWQLFTETS
                     IGRSQDNEIPLSEVSLSRRHCRIMYMNGNFVLSDLGSTNGCSVNGQPVWSQAWTLKDG
                     DIVQIGDVEMRLELKKP"
     gene            25391..26050
                     /locus_tag="VMZ53_00745"
     CDS             25391..26050
                     /locus_tag="VMZ53_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26995.1"
                     /translation="MTRLYRPALVAIALSACAMGGPGPRRGTVDANDDEGELADAPRT
                     SSTPDAPPASSCAQAFTGVLATWTLTGQAGNQASTPASATAPGVTAAALTRSAGLTAV
                     SGADSINASGWPTANMADLTKYYSFAVTPPAGCSLAISSVSIDLARSGTGPALGALAT
                     SSDNYAQQILVSTNTASTPSLVASGGTMQLQIRIYGYMASSAAGTLRLENTLSLTGSL
                     H"
     gene            26050..26355
                     /locus_tag="VMZ53_00750"
     CDS             26050..26355
                     /locus_tag="VMZ53_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26996.1"
                     /translation="MTCKRCGAETSRAWCRDCEQLYDGWSRRHASDIVWQALAGGVVV
                     ATVGMGLPLLGIGWLVAATGAFAGFGTLFGLSRLTHRRRRRQFLQSTLPRAYLTAPK"
     gene            26361..27158
                     /locus_tag="VMZ53_00755"
     CDS             26361..27158
                     /locus_tag="VMZ53_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUS26997.1"
                     /translation="MSPSFLYGTAWKEDDTERCVRDALAAGFRAIDTANQRKHYYEEG
                     VGRGVAASGIARSALWLQTKFTFIDGQDQRLPYDPRAPIATQVAQSFASSCEHLRTDY
                     LDSLVLHGPSQREGLGADDHEAWRAMETIAESGRARALGVSNVTPRQIEDLVAFARVK
                     PSYVQNRCYARVGWDALARKVCRDHGITYQGFSLLTANRQVLANKDVAAIASRHGRTI
                     AQVVFRFCQQVGMLPLTGTTDPTHMREDLAMDFELSPADIATLDAAG"
     gene            complement(27155..27937)
                     /locus_tag="VMZ53_00760"
     CDS             complement(27155..27937)
                     /locus_tag="VMZ53_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HUS26998.1"
                     /translation="MRWALPVVLLSGCGRLSFDATGDAANDDVSSVDALSGHDEDGDG
                     VPDLIDVCPHYPDPTQIDTDLDRVGNACDPQPAVSQQRWLFFSPLAGPDSRIVTTGQA
                     WTRGAEEWSVVSPNSGSNLFVAGAFKDIDVFVAIDLTSLAPTNRQLAIIIQNDAAMPY
                     YYGELFDGTGSGAAAVITQFDGTSYTGLNNAVYGAFPVGRTTFMHLIARMASATFTLD
                     VDGTVASAPTPTYTGAQNLSVGMGGCAGSVRYIAVVETLAAN"
     gene            complement(27967..28710)
                     /locus_tag="VMZ53_00765"
     CDS             complement(27967..28710)
                     /locus_tag="VMZ53_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS26999.1"
                     /translation="MKIGLVLGSLVLCGLSSAAYAQAPGDYYEGDETLAPPGMAPEVA
                     PDPAPAPPPVRQRRWSVGLNFGGLNVAPHTQPENESEFGVGQLALRYRPWRHLEIELA
                     LGGGREQLEDGTEGEREVSTSVLALRYRFNPQKRWNWWLMAGMGSFAVTNQWASDDEK
                     EAAVQSTLQFGVGLERRWHRFALQLEIRAVGVAPNEDDMTVYDGGGAVPTAPGETMEP
                     TPPPKDPTYSPAADGWKGGQTTIGASYYF"
     gene            28832..29584
                     /locus_tag="VMZ53_00770"
     CDS             28832..29584
                     /locus_tag="VMZ53_00770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUS27000.1"
                     /translation="MPGRTFAIGDIHGDLVALATLFDRLPPLVAEDTVVFLGDYIDRG
                     PDSAGVVRWVRELGKRIPAKVVCLRGNHEDAWLQVADEGWPEFILPRGNGCLECYRSF
                     ANLAVPDEEEAPTPEEFEQMLGAKFFPPDVLEWMRSLPFFYEDEHAIYVHAGIKREGE
                     GFPHPSATEPKRALLWLRDRDFFENYRGKIVVFGHTTTRTLPNELSTYTPDDPTDLWA
                     GPACVGIDTAAGKGGFLTAFELPAQRVYESRD"
     gene            29577..31850
                     /locus_tag="VMZ53_00775"
     CDS             29577..31850
                     /locus_tag="VMZ53_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HUS27001.1"
                     /translation="MTKTRSTGAPGSDAVAFRIGRALASPAGVLITVPVLVIAVGIGI
                     MLLGRNATRTSTHSMARHQLVEQAQSVQSDVAYALDQADPLIKLVSVLAVRDRPLDDT
                     LVRLHDLLAARPGIAFLSISFPDGTFRGAQYNASRQIEVQESLVSPSEARWFSVVDGK
                     LQLLRTEPHQYDPRKRGFYQLAVAKRARAWTEPYTFFRSHETGITCTEPLFDAMGNLQ
                     AVLTVDFDVGALSSFVARPGLDQARTLVFTADGVILAYPAADRLGLPTSDKLLRAEDL
                     RDPAIDALFAAKPTSTSRSQRFLDLDARDGEYLASVAPIGGKRAGIEVPLDWYVATVV
                     PARTLLGPTRKLERASIIASGGAMGIAVGLALILAWNLVRMRRQVQSAREEARSAEAR
                     ARELGSYRLVAKLGSGGMGEVWRAEHRLLARSAAIKLIRPDVLDSDGISEIRERFRRE
                     AQVLASMKSRHTIAIFDYGVTNDSTFYYVMELLDGLDLESLIVRYGAQPAARVIEMLI
                     QSCASLAEAHDHGLYHRDIKPPNLYLCRAADEVDIIKLLDFGIVQTVNELPRPVTRLS
                     AAPALETPKLTQIGAMLGTPGFMAPEQILGMQIDGRADIYSLGCVAWWLLTGNEIFTR
                     EGGEAKVLHRHIYEELPPLRERVRGWIPPELEAVVHACLAKEADDRPRDARELAKRLR
                     AIPIPDDYVWTAERAQAWWRSYSPPAAVPNLPSGEVQVIVPGRPSTHLPIAATKKDAV
                     VTIAAPTASTKLDIGRK"
     gene            complement(31851..33338)
                     /locus_tag="VMZ53_00780"
     CDS             complement(31851..33338)
                     /locus_tag="VMZ53_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27002.1"
                     /translation="MPGSHSPYGGCITAALFGLAAVVTSSLLAGCVEPPGADDMPATD
                     DPNSGLLRDFLDGKFDSAGHPVNARVTEAESLCASSGVPHNGSIRLKDKACQGTLGGS
                     EQHGDLVASVRLSVKAHAASGTIVSAAVLGVDGEILASSSLTTTNLRSASWVDLPLSW
                     SSEGQPVTVKVTPAPGAVIDLDYIEVFNERFGLVASPGSGSFVDSDKLVFELPRSHKL
                     DRVTLDGKDITTRISDLIAAGKAKRTTTAYRTLIELTVGDLALERVDASELQLRAGAL
                     AARVQLRRTPPACKFEGDPNGVKVLVTGFQPFPADGWHENVSAVAVTALDPSRLRNAQ
                     VMRVVMPVEYDRAAAEVTDLITRCSPRVVISFGQGGGEIALEEVAYNLQDTGEVAGGV
                     PDNRGIIRAAAQIDPAAPSTRNTLLPLDRIEEALQALGETPQHSRDPGRYICNNVMFA
                     DVGAMSSRGVAGFIHLPYTTQFDGASRERFGKVVQAAIQASVDSL"
     gene            complement(33456..34301)
                     /locus_tag="VMZ53_00785"
     CDS             complement(33456..34301)
                     /locus_tag="VMZ53_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HUS27003.1"
                     /translation="MSLAFRIINVFTVDGDRLSGNPLCVFEDARGLSTEQMQALARQM
                     NLSETTFVLPTDRATAHVRIFTPGFEMPFAGHPTLGTAHVVRAMRGGDRVTLEMKAGI
                     VEVSASGDAWTLRAAKAPETRKVDASRAELAAMVGLPADAVSEEPLWVNTGVEQLVIP
                     IASPDHVRAARPAPELIAKHGFSATRDEAMAYLWTRDGDELLVRFFFTQHGAVIEDPA
                     TGSACANLGGWMLATGKSLPVRETLSQGAAVGRPSRLGLHVDVDRNIYVTGSAVELGR
                     GTLSL"
     gene            34393..35214
                     /locus_tag="VMZ53_00790"
     CDS             34393..35214
                     /locus_tag="VMZ53_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HUS27004.1"
                     /translation="MPATVVDAVKTPYPCACRCHELLSFEERVAGIAALYRIDDAMRG
                     WGQTVIWDLAAPTLWRVQQQLGEVRWVTVRDGPCIHSRLLGFCVHELIHAMCGDPNAP
                     NWGTPVGLPYGVPESVPPTEEAAFLHPFNQHEARAWVGLGVVAERLFGIEWTLLPARD
                     VGTYGFPGGNALVDVPEGYRRVPHFDHKLHTRRYLALARKLEEEALAWFTTEKLDEIA
                     QRFDAAEKLGRAQRPFEFPSPKEMARLKPKQPGRNDLCVCGSMRKWKQCCGANVA"
     gene            complement(35211..36719)
                     /locus_tag="VMZ53_00795"
     CDS             complement(35211..36719)
                     /locus_tag="VMZ53_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27005.1"
                     /translation="MRAVVGALLLLLGCNEPPGLTLEVVPLDPSIEYVEVYLADHCDD
                     CPDAMTPPMMRAHTANMFETDYAQVFSASKADAVNWKNGHAFFRITSQDTDDYFLPYL
                     LIIGFDAARTPIALNRFAGVTIPASNADYWQVLLDEHVSPLTDAPSDEERIAIWGANR
                     TGPRCVLVVNGDGRADGVVPVDDPDCDGVMSHECAPYVPNAMHAAPRLEQANCVIPIQ
                     TPVFNATGPLQVCLLGGPACTDGGLPSVNCDPLDVDYCMPSKACGCFDWNTCTTQIDP
                     RNMPTVPFVNCLMAVGANGTECPDSAKRDAEIDLGMFISGGGGTKCTSIKVHELMLPL
                     GPFDDHIIVGGATFELANFQNCKVDVKWFDDYMRTLQGHSALLDVALDNGKHMLVPLL
                     VRPSELACESQSTCRVMQFDPNDTMLLCAKPAMDSTTCIATAAQCPDSAAACGARCCG
                     RGERCVDGECRCGDGTHCADDTFCVSAAMTNPPSSTCGDVCCSSGPGTPACF"
     gene            36752..39532
                     /locus_tag="VMZ53_00800"
     CDS             36752..39532
                     /locus_tag="VMZ53_00800"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HUS27006.1"
                     /translation="MRFVAIAVLVACSAPAPRRVPVTPGLPQPAQPTQPAQPPADAPV
                     SGDLGPAKPTLRLPKNFVAKSYTASLSINPAQPGFAGAIQITGEVSARSSVIWLHGRN
                     LTIKKATASRDGAAPTELVVKQADQDLLELRSAAPIEPGAYVISIDYAGTFATVETAG
                     AFVQVSGKSRYVITQFEALFARLVFPCIDEPDSKVPWQLTLDVPAKQVAVSNTPIAKE
                     SALPDGRRHVEFERTLPLPAYLVAFAVGPFDVVDAGKTKRGIPIRILALQGRAKDAAY
                     AASVTARIADMAEAWFEIPYPYAKLDMVTIPISVGFSAMENAGMITVTESEMLFDPQK
                     ISWANKHAYVSTAAHEIAHQWFGDLVTMVWWDDIWLNEGFASWLENKLTAQFEPAWHE
                     ELSDLDTRARALGADSIVTARRVREPIVTEDDIANVFDWITYLKGATVLNMFEAYVGA
                     EKFQRGVREYIKTRAMGNATSTDFIAAISKAGGVDVSAGFSSFLDQGGAPEIEFAVTC
                     GKGKPRVELAQQRYVGPGSPAPEANKPWIVPVCIAFDNAGKRGEACTLMSTETTSLEL
                     DAKACPRWVAGNANGRGMYRTRYTLAQATALRDEAWPQLTWTERRAVFAAVKTGTEGV
                     PRTMKKSVSKKPPAKLPLALALSFVPRMLAGGDRFTIGDATDLAGGFDWDVPEGQRAK
                     YESWIRATFGAGAAKVGLVAKPDDDLDAETVRKKLLGLVAWTGRDPDLTKQATDLAAG
                     WRDMASATRGLVLKIGADSTPDLHAKLMRDIYTEQDRARRGEMYYALAGVRDAKRYEA
                     ALELTLDPKLDFRESDTIIVAFSTEPMRQVAERFTRVHADALMQRMPKESATGEGRIF
                     IRVATRSCDESKHDEIEKFLRGTIGKLPGGKRSIDQGMEELDQCIAYRKVIVPELEAW
                     LSGVKLPKPAKP"
     gene            39583..>39993
                     /locus_tag="VMZ53_00805"
     CDS             39583..>39993
                     /locus_tag="VMZ53_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27007.1"
                     /translation="MRGVLAFAVLGISSTASAQSIAIDEFRPAMDARGYLTLNGSQTL
                     GHTEMSFGLGSLEWGKNLMSEDVGPGAVDNMVSATLVAALGLHAGPVPLELGASLPFS
                     IVDGSVDGQGIGDFGFHAKARVARVGRVGVGAIAS"
BASE COUNT         6819 a        13175 c        13066 g         6933 t
ORIGIN      
        1 cgtccgacag attcttctcg gtctcgccga gccacttcga gacgacctgc gagagatcga
       61 tctcgtagat gtcgaggccc agccgcttcg cgagcaccgt gccggacatc gtcttgccga
      121 cgccgggatg gccggagaac aagacggaga tgccttgcgc gccgcgcagc ttgaactgat
      181 cgcgaatcct gcgccgctcg cgggcagccg ctgcgatctc gacgagtgct gcgcgcgtgt
      241 cgtcgtcgag gatgaggtcc tcgaggtcgt acggcgtcgg cagcttcttg ccgagtctcg
      301 agatgcgctg gtgcagctgc gccgcgacgg catgatcgag atccgtggca accggcggcg
      361 aggagccggg catgcgtccc gcttcggcag cctgcgccgc cgcgacgatg atgccgcctg
      421 gaattgcgaa gcggcctgcg aggccgtcgg cgtcctgcgt cgtgagcgac ggcacgacgg
      481 tctgccacat cgctgcgcgc accggcagcg gcggaacctc gatgttcaag tgcacgagtg
      541 ggcgctgatg aatgcgcggc atgcgctcgc gattgatcgt gatcgcgacc ggcccttccc
      601 actcgtcgag gaacatgtcg aagaacgcgg gcatcgcgtc gcgatcttgg tcggtgacac
      661 tcgcatcatc gacgtcgacg aacgcgggga gcgcgtcgag cagcatgagc tcgcgcacga
      721 gtgaccgcac catcatctgt tgcaccgaca tatgcagctg cgagagccgc ttgctgtcga
      781 cgacgagcag gcgctggctc caatgggcag cggcgagctg cagcagcgtc ttcttgccga
      841 ggcctcgctg cccctgcacg gcgaggacga catcgttgct cttgagcgcg ctcgtcgcgc
      901 gatcgaccgt caccgcggga aacgatccgc tgaccgagcc accgcgcagc tccgcaactt
      961 cggcaagctc gggatcgaga tcgagatcat tgccgtcgag caagtagacg agccgcgtcg
     1021 ccgcacgaat gcgccggaac atcagccctt cattgggtcc gctggtcgtc agctcgagca
     1081 gtcgataccg cagcagcggc gagctcggcg acagatcgcg cgccatgagc gaccgtgact
     1141 gcggcgtgtc gtagacgagc tgcgcgagca atcgcccgtc gagaccacgc agtgagttat
     1201 cgcgcgcgag gtactgatag ccctgcacga ggctcggatc gacctcgggc atcagcgaga
     1261 acacgagcgc cgcccactgc cgttgcgaga ggttgtacgt gcggatcacg tcgacgagcg
     1321 gcgagcgtgt gcgcgattcg atcgttgcgc gaatgcgtgc ttcggtgtgc tcgtgtgcag
     1381 cgagccattg ctcgagcact tgctccgcgc cgctgtccgc accggcgact tgcgcgcctc
     1441 gtgcgaccgc gaacaaccgc cccatgtctt cgggaccgac ggcctcggca accgcgctct
     1501 ccttccgcca gccgaggcgg atctgatatt cgaggcgcag ccacacgcgg cgcagctcgt
     1561 cgcgaacgtg cgcggtgacg tcggtgtaag gctccacgct cacggtgcag cgagctttac
     1621 cggcgcggtc gtcgactgct cggccgcgag agagacctca tcgtccgtcg gagacttctg
     1681 actgcgcgtg tgtttcgccg cgtccatatc gatatcggcc gctccgctga tcgcgggatc
     1741 tttgtcgaac cacgtctcca tcgctgcatc gccgagcttc accgcgctcg cgagccggac
     1801 gtcgaacgca gcttccttct cgttgttctc gcgccaccac gacagctcga gcttgcgcag
     1861 cgttacgcgc gcaccgggtg cgagcttcac ggcgtacgtt cctgcgcgac cgaggaagcg
     1921 tccgatcgcg accgtgctgc ctccggcggg aacggtgagc tgctcgagct tgtcgacgcc
     1981 accaccgctg tcgggcggga gcttcgtggg aatgagcacc cagcgtggcg tcgcgtcgtc
     2041 gttcttcagc tgaacatcga cgagcaatcg atcgacgggc ggacggccgg gcttacttcc
     2101 gacgaccgtc acggtcaccg aggcgttcat ggcgctgcct ttctgcgctg tcggttcctc
     2161 gaccttgcgc tgcgtcttct gaggatccgc gcaggcaacg agggcaacga cgacgagcgc
     2221 cagtctcacg ccacggacaa taccgctatt cgtgctcgcg cgtcttctgg accttcttct
     2281 cgtcgagctc catacccgcc gtcgcgagga agccgccctt gcgctccgtg ccgccgatcg
     2341 cgaacggttg cgacttgagg atgtcctgct tcgtgaccgt ttcggggaac atgcccgact
     2401 tcggatcgta gagcggatcc ttgaacgagc cgtctgccga ttcgatgccg tcttccttca
     2461 gcatgcggtg gtcgtagtcg ttgcgaggcg cgttgtacgc gcgaaccatc tcgtcgacca
     2521 tcgcgtcttc ggtcgccgtc gcgccaagac ccgaccacag gcgctcgccc gcctcgttct
     2581 tgcccatgta gatcgagttc tcgccctgcc acgcaccacc cggatgcttg agcagatact
     2641 tcgggtgatt gtagaagtag taccactgtc ccgggatgag ctcctcgtcg agctttgccg
     2701 gcggcacggt gccgaactga ccaccttgcg acgcttcttc ggcgacctcg gtgctcttca
     2761 tgtagttcgc gacgggcgtg ccatccatgc cggctttgat gtgcatgcga ttctgcgcgg
     2821 gaatcgcttt gtcctctttc ggatcggcag agccgaacat cttgtcgaac ttcttgtcgc
     2881 cgatcgccgc acgcagcgca tcgatctcca tcgcgatgac ggcggacaaa cactcggcga
     2941 ccgttgcacc cgcgagccaa tccttcacgg cctgcgacgc gctgtggccc ttcttgagct
     3001 cccagtccat ccagcgaccc ttctcgtgcg ggttgatgaa cgagctcgcc tgcgtgtggc
     3061 ccgagcgata cttgtcttgc cagagctcgg gctgtcccgc gttctcgcag tggcgctggt
     3121 agttgtacca gtagtgaatg ccgtgattga gatcgttctt gccgccggag ccgggctcga
     3181 cgttggcctt cttcatgtcg ttgacgacga cgccgcccat cgcttctttc gtcgcgggaa
     3241 tctcgggctc gcccttcttc ttgtcgccga acgccatctc cgacgcagtc gcgccgctgt
     3301 ggtgttgcgc gccgctggat acgccgggga tctggccgtg actcgtgaac gacgaggtgt
     3361 cctgtgtcga cgtcgactct tgcgccggag cgtgactcgt cgtcgtctcg gtgctcggcg
     3421 tttgatggcg gcgttcggtg tcggtctcac gagacatagg aatctccggc ggttagggct
     3481 tccatgttac gggcattcac agcggatcct tccgcctata aatcctagcg atatcatcag
     3541 cttccgagac gaacttggag gctcaaacag tctggttcgc tgacgatgac gacatcgccg
     3601 tcccgcagct cgtggcgagt gatgcggccg cgcatgatgt cgttgacgta cgtgccgttc
     3661 gtcgtgccct gatcgatcgc gacgagcttg ccgttgtcga tgccgatcca cacgtgcttg
     3721 cccgatgcgc gcgagtcgtt gacgagcacg tgcgcgacgc ccggctgacg accgacgatg
     3781 atgccggtcg cggtgagcgc gaagcgttgc cccatcagct gaccgcgcgt gcatgtcagc
     3841 gagccgatcg agagcgagcc aaaagcggtc ggcgcgatgc cgcctgattg tcccgggccg
     3901 ccgatcgcca tcgtctttgc aacgggtgcg tggcccggct gcattgcggg ctgcggcatt
     3961 cccggttgcg gcattccaga cgccatgtgt ccgggctgaa cgcgaccgta catctgcgga
     4021 cccggcggca tctgcggcgg cgcgtgcatg ccgggatgcg gcggatgcgg cgaatgcatc
     4081 gggggatggc cgtgcatcgg catgccgtgc ggaggccgcg gcggtccttg ctttcctcga
     4141 cgagagatca cgatgccgcc gatcgctgcg agcacgagac cgccaacgac cagcccggcg
     4201 atgaggccgc cgttgctctc gggcttcttc ttctcgcgac cttccttctt cgcgcggttg
     4261 ctctggatga tgtagctctg cgcttcgtcg tgctgcggga acagcgtctc gacttcttgg
     4321 aactgctcga tcgcggcggt gtactcgtcc gcccagaagt gccgcaggcc tttgttccac
     4381 gccttcttcg tctcgctctc cttcggctcg accttggcgt cgttgaccat ctccatcagc
     4441 gtcgaggacg cgacgaggaa gttgaagccc tggacttcgc tctcgttgcc gaacgtggcg
     4501 agcccgacga cgttaccctt catgtcgagt gcgggaccgc ccgagttgcc gtgcgtgatc
     4561 ggtgcggaga tctgcaggat ctgctcgccg ctcgatgcgc gcttcagtga cgagatcgag
     4621 ccgtcggtga ccgatgcttg gagctgcgat ttctcgtcga gcagcacctt catatcggca
     4681 acgccgggat agccgatgac gacgatcgga tcggcgaccg tcactttcgc ggagtctgcg
     4741 atcacgagcg tcggcgcgtt ctccgtcttg accttgatga tcgcggcgtc gcggccgctg
     4801 cccggtttgc cgaacgattt gatctcgtag ttcagcttgt cgccgttcgg cagcacgatg
     4861 tacgcgcgct tctcgaagtc gacgagcttg atcgcatcga cgaggcgctg cagatccgcg
     4921 ttgctcatgc gcgagatctc tttctcgtac gccttgttga tgtcggcgta gagcatcttg
     4981 agcagtgcct tcttcgccgc ttcgtcgccg tcgtgaatgt cggacacgac gtgcgcgttc
     5041 gtcgcgacgt acccgtcggg cgtcatgaac cagccggtgc cggtgccgcc gacggcttct
     5101 tggactttct cgccgaacag ctcgtacgtc gcgatgtacg cgccccagac gcgcacgacc
     5161 gagggcttcg cttcgaccgc gactcgctgc tgtggggtcg gttgggcgtg cgcgacggtc
     5221 gcgagcacga caacaaacat gcacgcaaga cgcgtgacca aacccatcgc gccgatggta
     5281 ccgcacgggc ccgcagcacg ggtatcctcg ggccgtgggg tttccgttcg cctatcgcgc
     5341 gaagagccgc aaaggtcgcg gactgtttcg gaaggctcct gatccacgcg agatcgggga
     5401 ccgcttcggc cggttgctca agcgcatgca aggcagcgcg gtgcttcgcg cggcgtggaa
     5461 aggcgagcgc tatctcgccg agctcgagat cagcccgctc gtaccggcga tctcgctcgt
     5521 cgtcggtgac gatgcagagc tgacgatcac cggcgacgcc acgtttgggc ccgcggtgat
     5581 cgccgacgtc gcggcacgca tggcgccgat cttcgaagag ctcgacttcg tgtgggtcga
     5641 cgaggcaccg tcgatcgaga ctgcgcaggc gatggtctgc gcgaagctgc gagagacgct
     5701 gcagaagagt gacggccgcg tgcggatcgg cgtgccggcc cgaaaattcc tgatcgatgc
     5761 gccggtgctg acggcactcg gtccgcgcga tgcagcgtgg cgcgatggcg tgagcgccga
     5821 accgtcgcgc gcgcgtgatg cgtttccgta ttgggagccg ggcccggggc gcgctgcact
     5881 tgcgcgggca ctcgtcgcga tgtggctcga gattccgtgg cgcgagccgc tcgatcgcga
     5941 ggagcgcgac ctgttcaagc aagtcgatga ggatctacgc gctgcgcgta aggcgaagct
     6001 cgatgtcccg tggcgcgcgt ggcaggagct cctccgtcac atgggcgtcg aggacgaaga
     6061 cgtcgacgag aaagcgaaag acgcgaccga tcctccgatc ggttatcgcc gccacgatct
     6121 ggaagcagag ctctccggcg gttggtccgt gcgcatgtcc ggcgcgatga tcggtcattg
     6181 ggaagatgat ggggcgcgct actgggcgac cgatggcgat cgcgttctag agttcacgag
     6241 cctcaccgcc gaggaagaac gcgatacgga caagttgctc gcagtcgcgc ccgaagcgca
     6301 tcccgtgatc gcgaggttcg cggaaggcga gcagcgcggt cgcgccgagg cgttcgagga
     6361 cgatggcgtc gcggtcgtgg tcgggctcat ggcacgcgca ccgcacgtcg gcattctcac
     6421 gtgcaaaggc ggagagcgag actgggcgct cgcgacgtgg cgcagcttgc agcacaagga
     6481 ttagttcagc ttcagcgtcg ccgacgcacg ccacggcgcg tttggaatcg agagggcgag
     6541 gtcccagatg ccttcttcga tgcagctgcg cacggtcgcg ctctcgttct gcgcgttgac
     6601 gtcgacgtag atgatctctt cgcgcgtcgt ctgcagacgg atgacgagcg gcgtcttggc
     6661 cccgcagcgc tcgacgatcg gcgccacctg cgctttgacg tcgacggttg gatgcagcac
     6721 gcccgtgatg ccggttgcga agtcggacgt gctgcagcaa ccgcccggtc caaagccgct
     6781 gcgcccgaaa ccaaagcccc cttcggactg accgagaggg ccgccccact gcccgaacag
     6841 cgaccacgcc tcgttcactg caaacgccgc atggtcgatc tttgcgcgga gatcgtcgtc
     6901 gaggaaatgg ctgctcacgg agagctcgcg cgcgagatgc cgggccgacg tcgcgtccgg
     6961 aacaagcgtc ttcgtgagct tgtgacccca cagccacgcc tccacggtga gcgcacccgc
     7021 aatcgtcgtt ccctgccccc accacgtgca cgactgccct tcgctcaccg tgtcgcggca
     7081 cgtgccgatg ccgaggtctt cccagccgcg tcctttgatc gtcagcttgt cgatgctgag
     7141 cggacggacg agcatcgtcg cgtcgagcgg ctccttctcg tcgacaccgg cggcgactgc
     7201 gatgccctcc gtgagctcgg cgatgcctcc gagttgcgtg cggtcggtca tctgcatgcc
     7261 gagcgtaccc gcggagacga caacgacatg gaccagcgtg tgctcgggaa gcaaggcctt
     7321 gatgtcgtcg ccggtcttcg cttcgatggc gctcgcaaat cgatcgtcgc tgatgatgat
     7381 gagccgccgc gtgccgttcg cgcgtgcgag ccagcgaccc gcttcggcga tcgcgacgtc
     7441 gacgttcgac ccgttgcgcg gagcgagtga gcggatctcg cgatccacgg aagcgcgtgc
     7501 cgtcgccgca ggcatccacg ctgcaagcaa tggcttcgct tcgcgcgcgt acgcgatgac
     7561 ctgcactcgc gactgcgcag gtacgtgctg caagtacgat gcgaccaccg cacgctccgt
     7621 ctccacttca tcgcccgata gggatcgcga atgatcgatc acgatcgcgg tgtgcaagtc
     7681 aggcggaacg cgactgagct cgctcgctag atcgatctcg acgcgcgcga ggtcatcgtg
     7741 cccgagcgga agccggcccg cgaatgcacc gatgcgtgtc tcgccgaccg gtcgccgcgc
     7801 gagcgtcggt gacgcaaacg agatccactt gcgttcgttc gggacagcgt cgggtgcaca
     7861 gacgtcgcga agcgcctgct cgtcgtcgat ggccggtgcg ccggcgagct tctcgagcca
     7921 tgtctcgggc acgcgcgcat agcggcggtc gcggaagaaa cagcccggca cctcgaccgt
     7981 gagctcgagc gtgatgctcg tcgtggcctt cggcgaaagc acatcgagcg tcacgaagcc
     8041 gagcgtgccg ctgacgtgga caaaccagcg gtggttaccc ttcggtaggt tctccgacca
     8101 aacaccgtcg agctccttct gcgcggtctg cgtgtcgacg agcgcgagca catggcgagt
     8161 gccttcgacg gtggcaacgg cgcgcgtcac gatcgagtca tctggcgtgg agatgggcac
     8221 cccgctccag atcgatgcgt cggcgacgag gtcgtagcgc gccgtgagct cgatcgtggt
     8281 tccggacagc cgagcggtca cgcgcgaagg cggcggatcc tcgtcgaatg cgcgggcgag
     8341 agaaggccac gccgcaagca ggacgagcac ggcgagccag cgcatggccc tgagacagca
     8401 ggaaggggtg ataggtttcc gcgaatgggc aaccagtatg ggtgtggccg atggcgctga
     8461 tgcaactgct cgaaccgata gtgatcatcg tcccggtcat gctctggctc atcgtgatcg
     8521 ggccgctggt cctctatccg atggcgcggt ggaaggcgaa ccgagaccca catccagacc
     8581 cgcagctcgg gctcaaggtg gcccttcatt acttccggat gatcgcgttt cagctgctgc
     8641 tggtcggcgc gctcatcgtc ctgtgggcgc tgatgcgaaa gacgagtgag aatcgcggcg
     8701 acctatatcg cgcgggcttc ggcctgctct tgccggcagg gctcgtgttc ggtgcgcacg
     8761 tgatgttgct gatgcgcacg aacgaccacg tgctcgtcac ggtgcggcgc ctgttctttg
     8821 gctacaacct gctcgtcacg ggcctgttcg gtgtcaccgc gctcgtgctc gcgtttcaag
     8881 cgctgtttgc gaaaggcccg tcggacaacg aaggccgctt ctactatgcg gcgctcgtcg
     8941 tctacgtctg cgcgtgggcc gggtgcgcgt tccactacat catgatgatc gatcgcagcg
     9001 cggccgctgg gcctcggcag aatatggcgc cgccaccacc gatggcagca gcggcaggtc
     9061 ccggattgcc gtcgctcggt ggcgggtttc ctcccgtggg tcagtgaacg tcgatgcggg
     9121 cggcctggat cgtcgtcggg atgaagcggc gatcgacgat gtccgataga tcgatcggcc
     9181 gcttcaagat gcccgctttg atcgcgtacg tcatgatcga gcgcagatcc tcgtcgctcg
     9241 gcgtgagctg ccgatagctg acgcgatcgg gcggctgcgt gagcacgaac tcgagcagct
     9301 tctgatcctg gcggtagtac ggcgctgcga ccttcgcggc atcgaggcgg tgctgctcga
     9361 cccactcgcc gctctcggcg ataccgcgca cgaggtcgcg cacgacatcg ggatgctcgg
     9421 cgatgagctc gtcgcgcacg acgaggacac acgagacgaa gtgcggccag atgtccttcg
     9481 cgtagtacag aacgcggccc gttcccgcga gctcggcctt cgcagcgtac ggctcgccga
     9541 tgaagtatgc gtcgatggcc ccggccgcga gcgccgatgg catgtcggga ggcgccatgt
     9601 cgacgaagtt gatctcgtcg ggcttcatgc cttcgtcttc catgagcttc gtgatgacga
     9661 ggtactgatt cgagaaccgg cttgggcgcg cgaacgtctt gcctttcaga tcgcgcagcg
     9721 acttcgccgt cgaattcttc gggacgatca ccgtcgagcc atcgcggtgg ccgagataga
     9781 cgatgtggat cggcacgccc tgttcgcgca gcaccatcgc gagcggcgcg aggatgaacg
     9841 tcgcgtcgat cttgcgcgcc ttgagtgcct cgacgaccgt cgggaactcg gtaaaccgct
     9901 gcgagagaaa gcgcgtgccg gtcgttgtcg tcttcgtcga ataatcggtc accgggcacg
     9961 tgaggtgaca cgtcacgggc aagtagccga cttggagctc ggccgccgca gcgttgcgag
    10021 tcttccacgg cttcttgtga gcgagcgtga gcccgacgag aaccccgacg gtgatggcga
    10081 tgaggcgaac tttggtgctc atgacagtcc cagctcctcc atgatcgtct gttctgcttc
    10141 ggcaacgccg ggtgcagcgg gtgcgcgtga ccgtccaggc ggaagcgcga gctctttgcg
    10201 cacgctggcg gggcgcgttc cgagcacgac gacgcgatca ccgatctgca cggcttcctc
    10261 gatatcatgt gtcacgaaca cgacgctgcg aggccgtgcg gcgagcgtcg acacgagctc
    10321 tttgcgcatg cggcggcgcg tctggtaatc gagcgcggag aacggctcgt ccatgagcaa
    10381 gatgtccgtc tcggcagcga gtgcacgcgc gagctcgaca cgctggcgca taccgccgga
    10441 gagctgatgc gggaagctcg ccgcgaactc ggtcaggcgc gtgatctcga gccactcgtc
    10501 gaccttcttc atgttgccgc ccgtgccaag gccgatgttt tggcgaaccg ttagccacgg
    10561 gaacaagccg ccatcttggt agatcgtgcg cgtctggccg ctgcgcttga tcttgccgcg
    10621 cgtgggagca aggtgaccgc cgagcagcga gaggagcgtc gacttgccgc agccagacgg
    10681 cccgacgcac acgagcagct cgttgcgagc gacgcgcaga tcgagcttgt cgatcaccga
    10741 caacgagccg tagtcgaagc cgacgttctc gacctctaga acgagatcgc tcatcggtcg
    10801 aatccccagc gcaggctcgg aatgcgcgcg aggcgcgaga acaggatatc gaggctgatg
    10861 ccgatcgcgc cgatcgtgat catgccgacc acgacgaggt ccatgcgcag accgttgcgg
    10921 gcgtcgatga tcaagtagcc gaggcccgac ggcaccgcga tcatttcggc gacgacgacg
    10981 accatccagg ccacaccgat cgcgacacgt agcgcggtca cgagctgcgg catgatcgca
    11041 ggaagcgtga tctggaagag cacttgctgg ccgcgcatgc cgagctcttc ggagacgcgg
    11101 tagtagacct tcggcaccga cgcagctgcg gcggcagtcg cgagcacgac ttggaagaac
    11161 gtcgcgatga agatgatgaa gatcgccggc ggatcgccga caccaaacca gatgatcgcg
    11221 aacgggatcc acgcgatcgg agacagcgag cggaagaagt tgatccatgg caggagcgct
    11281 tcgcgcgtca tcgtgtggcg accgatgacg agaccgagcg gcacggctgc gagcaccgcg
    11341 ctgataaagc cccacgcaac gcgaaacagc gacgagatcg cgtcttcgag caggcgcccg
    11401 ttgcggatct cggtgccaaa cgcggtcagc acgtcgatgg gcctcggcac cgtgcccgtc
    11461 gggaacatgc cgagctccct cgcgatcgcc cagatcgcta gcaagacgac gccgcacagg
    11521 agcggcaaca cgacagccct cattcgcatt ggccgcctat cctgacagaa atcaggccct
    11581 tcgcgaatag ttgacagtag gctgacaaac gagcggcgta tcacgagaac tatgcagctc
    11641 gcgataccca cgatgaagcc gttttcgttc gcgcagacgc tcgcctttct cgagcggttt
    11701 cctccctgcc gcggcgagta cctcgtcgag gacggccggt tgaccgcggc agtcagcgtc
    11761 ggcggccatg cccgcgcctt cacgctgttc gagcgtgccg gccaccttta tatagataca
    11821 gacgacgtgg cgacggtccc gcatgccagc cacttcattg gcgccgccga caacgtcgct
    11881 gcgttctatg aagccgcgcg cgctgacaag ccgatcgcaa cgctcgtcga gctcctgcac
    11941 ggattgcatc atgtgcggtt tctgacgctc gccgagatcg ccgtctactc ggtgatgatg
    12001 cagcgcgcac cgatcacgat cgcggctgcg ctcaagcgga agttcctcgc cgcgttcggc
    12061 aagccaatcg acgttcgagg tcacacgttg cgcgcgatgc ccgatctcga cgagctcgcg
    12121 acgctgcccg tcgatgcgat cgcagacgcg atcaggcacc gaggcaaggc cgagcgcatt
    12181 gtcgaggtcg tcgcgggcgt gcgcgccatc ggcgaagcga cgctgcgcga agctccatac
    12241 ggcgaagctc gcgacgcgtt gctcgcgatt cgcggagtcg ggccgttctc ggccgccgcc
    12301 atcctgctgc gtgggctcgg gcgcatggac gagctgccgt ggctgccggc attctcggag
    12361 ctcgctcgca agcactacgg ccgtgcagtc gaccacgcga cgatctcgcg gcgctacggc
    12421 tctcacatcg gctactggtc gttctatttg atgaccggcg caccgaggct cgctaagctc
    12481 gctgcgtgag gcgcgtgttg ctggttgttg tcgtcggcgc gtgccgcttc aacttcgaca
    12541 accagagcgc aacgggagat gccggcgacg cgccgatggt cgacgtgctg ccgcccgcag
    12601 acttcgtccc gaactggcag aacggctcgc gaatccgcgc gatcgtgttc cgcgcagtcg
    12661 aaggcggcga cccgcactgg ttcaactggc gagacacgca gctcgactcc gattgctact
    12721 tcggcatcgg cgcggatggc gtggagcgct gtcttcccta cttcgcgagc gccgaaacct
    12781 tctattcgga tgcggcgtgc acgcagcctc tcatctatgt cgccaacaac gcgtgcgacc
    12841 gccacatcaa gtacgccgac gacatcgttg gatcacgcca ccacatctat ccgatcaacg
    12901 gcatctacgt cggacaggcc tacgacatcc gcacgggctg ctcgcctgca accacgccgg
    12961 gcggcacgct ctacaacgcc ggtgccgagg tcaacccgac gatgttcgag ccggttatct
    13021 atcacaccga gatcgtcggc gacttcgagc gcacgtttca gggcttctcc gacggcgcgg
    13081 cgatcgaaat cggcaacctc aacttcaaca tcggatcctg ctacccgtac ggtggattcc
    13141 tcggctcgtc gcggtgctcg cagtccgttc agttcatcgc agagccggtc tacaccgacg
    13201 ctgcctgcac gcagcgcgcg tacctcgcac aaacctcgac gacgtacttc agcgtccgcg
    13261 ctgctgaggt ctgcggcgcg agctactcgc tgtacgtcac caccgccgac gtcacgcagg
    13321 cgagctactt ccgccgcacg ccgtccggct gtcagatgac cactacgccc gcgggatccg
    13381 ttctcctcca aggctcacca acgacgaatc cgtacccgac cggcaccaac atcgttggtc
    13441 cggatcgcgg acgactcggc accctctact gggtcggccc agatggctcg gcgatgatcc
    13501 tcggtacgtg ggaccaacag ctcgatcgtc cgtgccgccc gttcgtcgca gccgacggca
    13561 agatgcgctg cctcccgagc gcaacgccgc gctctctcgc ctgggcgaac gcgtcgtgca
    13621 gcaacacgca agacaacctc gccgtcgagt gtttcccggg aatcgcgccg ctcgacggtc
    13681 cttcgtaccg ctcgtgcgaa gacacgccct ggagcgtgcg aacgctcgca aaggtcaccg
    13741 acacgccgag catcgcgaac gaagcagcgt gcgtctcgct ctccactccc gcatacgacc
    13801 cagccgttcc ggtagctacc ctttcgccgt cgatgtttcc cgagctgacc ttcaccgtcg
    13861 aataaaccgc ggtgctacgg tcgcgcgatg aaaaaagtcg ccgtcctcgg atccggtgca
    13921 gtaggtgatg tgctctcgaa cggtttcctc acgcacggct acgccgtcac gcgcgcgacg
    13981 cgcgagcccg ccaagctcga agcctggaag aaggccgcca agggcgaagc agccatcgca
    14041 acatacgcgg acgcggcgaa atgggccgac atcatcgtcc tcgctgtcaa aggctcggtc
    14101 gccgaagaag tgctcgatca atgtggcgcc gcgaacctcg cgaacaagac gatcatcgac
    14161 acgacgaatc cgatcggcaa cgagccgccc gatcacggcg tcattcgcta cttcacgaac
    14221 gcaaacgaat cgctgatgga gcgcctccag aagaaggtgc ccgccgccaa gttcgtcaaa
    14281 gccttcaact cggtcggcaa cgtgttcatg gtcaacccca agttcgagac gacgccgacg
    14341 atgttctact gcggcaacga caccgccgcc aaagccgagg tcgccgacgt cctgaccaag
    14401 ttcggccacg agccattcga ttgcggcggc gtcgaagcag cgcgtccgat cgagtcgctc
    14461 tgcatcctct ggtgcatccc gggcttcatc cgcaacgact gggcccactc cttcaagatg
    14521 atcaagcccg cgaaatagca ggcccaaaat ctaaaggttt acaaaaccgc cctctttaat
    14581 agagtgcgcg gctcctacct cacggagcct ctcgaatgcg cacttccatt tcgctcgctt
    14641 ttctcctggc caccgcggcc gccgccgccc ccaccgccgg ctgtggcacc gattccaccg
    14701 tctccggcgt attccccgcc gaaggcttca ccggccgctc gcttcgcgtc gagatcagcg
    14761 gtgacgcaac ggagtggtcg ggctcgcccg ccgtcaactt cggccaaggc gtcaccgtca
    14821 gcagcgtgtc ggtcgcaagc ccgacgtcgc tgttcgccga catcacgatc gcggccgacg
    14881 cagcccccgg cctccgcgac gtgacgatca caaatgacgg caccttcaca ctgaaacaat
    14941 ccttcgagct cgtctcgccg atcacggtct cgttcgacgg catggtcgct caaggcggca
    15001 gcccgtactt caccgtcacg aaccacgact tcgacacgcc gttcgatctc acgtccggcg
    15061 atacgccgag cggcttcgcg aacctgacgc tcgatacgcc cgcgggcacg caattcgtcg
    15121 ttcaggactc gaccccatac cagctcaccg gcttcgcatt gatcgatgcg gacgctgcgc
    15181 ccggtacgtt ctcgctctcg agcggcgtga tgggcaaaga gaccgcgttc aacctcggtg
    15241 caaacatcga catcacagcc cgcacgccga cgccgctcac ggacagcgcg acgggcacgc
    15301 tcgcttcgat gggcgactcg gcgctctact ctgtcaacgt cgcgaccgca ccgtccctca
    15361 tgcgcatcgt cgcggacacg tcggatgccg acggccaacc cgtcacggcg gttctgccga
    15421 atggcagctg ggccgatgcg accggcgcag cgctgtccgt ccaagcaacc tccggcacgc
    15481 tcgacgtcgt cgtcttcgat gctggaaagg ccggcggcta cagctacacg ctgaagagca
    15541 agatcgaggc gctcgcgtcc gctgcggaag caacgacggc gaacgatacg ccggcggccg
    15601 cgctcgctgc gtccgcgctg ccattcgcgc aaacgggcgg gacattgtct ggaacaaacg
    15661 ataaagacgt catcaagatc gtcctctctg cgccggcagt cgttcacgtt tcggcgcatt
    15721 caagcgacga tctgacggac accgcaatcg acatcctcgc gaatggctcg ggtaacccga
    15781 ccgtcctcac gaactatgtt gatccaggac ctccagctca gccgcccgtt gacctgggcg
    15841 gctgcttccc gctgttcggc ctcggctgcg gtgaggacat cacgagcccg atgctggcgg
    15901 ccggtaccta tttcgtcgtt atcagcgccg gtggttcgtt tgacgtcgct gacaagaact
    15961 acaccgcgct catctacttg aactagtgac gctctaccgt ctcgttcgac tcgaacggcg
    16021 gtgaaatctc cgaaggggtc ccttgcggcg taacaccggc gggcgtggcg gttcgtatga
    16081 acccaacgct gccgccgccg ccgccgggcg ttggaggata cgttgttgct ggtccattca
    16141 cgcgtccacc tgagaccgga acggctgtag cgcaagcacc tgctccgcct gcgccacccg
    16201 cgccgcccgc gggacctcca ggcgcgcagc tcacggtgtc gatcgcatcg gatccatcgg
    16261 caccgtccga ggcagtaggg atggtgttag ctcctttata gccggcgcca ccgcctccac
    16321 cgtttgcaaa aagcctcccc gtaacacgta tgcggacgcc ctgaagaaga atgtcaccgc
    16381 cagcgccacc cccgacgccg ccaaacacgg tgttcgatcc ctggacgtaa tgtccacctt
    16441 ggccgccctg cccattcgcc gaaactgttc cctctacgtt gacctcacca ccgcacgaaa
    16501 tgatggtgat cgcacctcct ccggtcccgc cagcagtcgg tccagccaat aagacaggcg
    16561 agctcagcgc gttcacggcg gcaccaccgt tttgtgcacc gccgcttgct ccgagcgatc
    16621 cgcctgcacc accagacgtc ttgaatccgg cgccccctcc gccacttgtt gcggtaggcg
    16681 cgccaccgga tgacaaaggc ggcccagcgc cggcgccgta gctggcgaca cttccagaga
    16741 ttcgtatatc gccgtcgcta acgattgcca gcgcacgact gccgttcgtc gaaaccgtca
    16801 gcccactcgg gacaacaacc gaaatgaaat gcacaacgca gatatcaggc ccaccgggct
    16861 gcgcgacgat acggccgcat tgctgatcgt cgctggtcct cacaactaga tcagcatcga
    16921 gtgtcaaagc ctccgttgca ggcgaatcgc aaacgtccgg taagtacttc ggcgttagca
    16981 aacctggacg acattcgccc gcgctcacca agcagaagcc gctagcgcaa tcgctatcgg
    17041 cggcgcaggg ccgacacgca tggctttgcg agtcgcacac gtgcattccg cattcagcat
    17101 cgctcacgca ttcgacgcag atgccatcac tcgtaaggca cagcggtgta gcggcgctgc
    17161 agtccgagct cgcttggcag ctactcggcg gagtgcaggt gttctgcacg cattgaaagc
    17221 cgtcatcgca accggtgcca ggcggcagtc ctttcgccat gcagtcagct tcggtggtgc
    17281 agcaggcggc cgggttgtgt ttggtgcaac cggacaggaa gaaacagccg acaacgaaaa
    17341 cgacgaccaa cccaacgcgc tcactcatcc cgagacgata accgactgag cgcctacgtc
    17401 gctgtgacaa aaaaagccgc cggcgtctgt gcgccgacgg cccgactcac gcccatgcgg
    17461 caagctgcgt gagtcaaaga cgtaagacgt gcaggccgag cgccagcgcg tacgtcgcgt
    17521 gagcatgcga gctgatctga gtcattggcg tacaacgccg tgctgcgaga tcacgacgag
    17581 cgacgcggca cctgctcgca cacgcgtgaa acgcggctct gcgggtactt gggggctggt
    17641 acgtcgcttg ctgaactctg ggaacaagcg gcaagcggca acgctcctcc gccgcctgcg
    17701 cactcagaac ccgcagattt gcggagaggg aattgtatct atgcgtctcg tgaaagcact
    17761 tctgaccact cttgtgatga tcacccccgt cgtggcttcg gctgacgaca cccttggcaa
    17821 cccggcgacc ggcgctcctg gcgtctcgcc cgacaacgag aagactggta cgccggcgtc
    17881 cgatgcgccg ccgacgccga ccggtcagtt gccgcccgcg aagtcggatc atgacccggc
    17941 tccgcccgtt ccgaccccgg caatcccggg cgtgaacgcg gacgtcgtcg agcaagcagg
    18001 cgtcggtggc gcggtcggtt acggccgcgc tggcgtcctc gagctcggtg gctcggccgg
    18061 tctgacgatg gcgcaggacg ttcgcgccct caacttcagc ccgtcggttg gttggttcct
    18121 tatcgacaac ttcgagatct cggcgattct cgacgtgacg aacctgaagg cgggcggcga
    18181 gagcgcgacc ctcttctcgg cactcgtcga gccgagcttc cacctcccca tcaaccgttc
    18241 gatgttcggt ttcgtcggta tgggtatcgg tgcggcgtac cagcatgacc tcggctcggg
    18301 tatggcggtc gctcctcgcg tcggtatgga cttcctcgtc ggtcgctcgg gcatcctgcg
    18361 cccgtcgctg gcgtacgaat acaccacgca cgactcgatg gccgcgattg gcccggacgg
    18421 ttcggcgaac gtcacgctcg tggcgatctc gagcgcgctc cgcttcaaca tcggctactc
    18481 gtcgatgtgg tgagctgagc tagcagtctg aaccaagact gtggagtgaa catcgagccg
    18541 cctgggaaac cgggcggttc ttttttttcg gcgtttcgtt tcggtggcgg ttcagttgcc
    18601 ccacgccatg ccgagaccga agaggctgcc gatgccgtcg ggcaccgcga ccatgcggaa
    18661 gagcagctcg aactcgaacg tgcgcgatgg cgagtcgtcc atgcgcgagt agacgtcgta
    18721 gccggcgcgt aggccgatga agccggtggg gagcgtctgc atgccgtgca tgttgtcgtt
    18781 cgtgatggcg aggccgccac cgagctctcc atcgatgaac gcgcggaacg agtgacggcc
    18841 ttttccgatg agctcgcggc gaaggccgag catcgcgcga tgccctgtgc cgtggcgctc
    18901 gggcggcggc ggggcaatca tgtacgttct ttcggtcgga cgatcgaaca accacatcca
    18961 ctcgtactcg ccgaacatcc gcgtcttctt gaagaccggg tgctcgacgc cgagtccgag
    19021 agacacggtg agcgttggcg cgccgtcaac ggggagtgcg cccatcgcaa agcgcatgtc
    19081 gagcatgttg aagccggggt cctccacttc gtcgtccgca tacgcgttgg cgacgccgag
    19141 gaggctcgtg catgcgaaca ccgtgaggaa aatgcgagtc accatgcgaa accaaacccg
    19201 gtgatgaagc ccgtgctcgc gcctgcctcc atgtcgcaac gaccgcggca ctgcacgagg
    19261 gagctctcgt tgctgttcgg cgtgaagaga atgcgcgcgt cgaatcggaa cgcgaggcga
    19321 gggcgcttgt acgtccgcac ctgcatcccg aaaccggcag caagctcggg cctcgagagg
    19381 cgtccaccgt cggagaaata gaagcgctgc gcgccgagtg cggagagtag gaagagctcg
    19441 atgccgccgc cgctgtccgg acggaactga cgcgcgagcc aacgcacgcc gatgtcaccg
    19501 cggatgactt tgccctcgat gcccatgtcg agcgatggat cgaccgactg cgagtgacgc
    19561 gtcgtcttcg agagcgagaa ttcggcgatg tactgccagc gatcgcgacc gatcgcgagc
    19621 tcgaggtttg ggccgacacc gctctccgat tcgccgccga tgcgcgtgcc gtgaatgacg
    19681 agtccgaggc cgacgctgcg tttggggaat ggtacgaacg cgccatcttc gtcgtcgatc
    19741 gtctcgtcgg cgtgcgcgac ggcggggacg gcgagcagaa tggcgaccag ccacagatga
    19801 gccctcacgg tgagggctga aaacgcacga caccggggtg cgatctactc ttggctcgaa
    19861 tttatgggca ggaggtcatc ccgctgatcc agctgcccaa aagctgctgc gcgagcggat
    19921 cgacgacgac cgttccgagc gccggcatgc ccggacggct cgtgactttt tgatagacga
    19981 ccgagctcgc cgggtccccc ggcacgatca cctcgcagag ctttgtctgc gcgagcggcg
    20041 tcgtgtagcg caggtctttg atcgagatgt attgagggtt gtggcagtgc tggcagttgc
    20101 ccgccatgta gccacgcatg cggtgctccg ccgtttccga cggttcgtgc ggcgagacga
    20161 gcggctgggt cgacgaattc gggccgatgc cgagcgcggc gagcgtgggg agttgcggcg
    20221 cgatcacgcc gtcgtagtcg ttccagcgct ggagctgctc gggacgaagg ccgagcatcg
    20281 ggcccatcgc cgggtagtga cacgagttgc agtgctggcg gctcggatag acgtgcggat
    20341 gcgcggtgcc gtcgtcgagc gtccagttga cggtctgtgc ggtgtccgcg ggctgcagcg
    20401 tcgcgtcggt gcccgctgtg ttccagcgat agctgaagcc tcgccaaccg ctggtcgcat
    20461 cattgatgag gaagcgcgtc tcgacggggc ggcgcgtcgc tggatgtccc ggcgtcgtct
    20521 cgatgtcgaa ctctttgacg atgatcgagc cgaccggcgc gctccacgag ccgttgctgg
    20581 cgagcgtcat cgacgcgtta tcaggcaggc cgatccagcg acgcttgagc gcaccgtcgg
    20641 accacagcgg cgacacgatg tcgtacggaa cgaggcccgc gtgcggcacc ggatggttcg
    20701 cagtcgcgag cgagaagcag ccggtctgcg agaggtaggt cggcggcgcg gcgacggaga
    20761 atccgcagcc atcgatcgat gcgcgcgtgg cgaggccgag cgacggcgtg cgcggcccgt
    20821 tgaagcgagc gatcacggcg gttcccgacg cggcggttgc gacgacgggt acgtccccgc
    20881 gtgcgagcgc gagctcggtg cgcagcggaa cgccggtcgt gctcgcgagc cacgtgatgc
    20941 cgccgacgac ggcccacgat gatccggacc accgggcgat cacgccgcgc tggttctctt
    21001 cgacgagctc ggtccacgcg acgatcggtg cgcctgatga atcgagcgcg acggccggcg
    21061 tgaccgcgtc accttccata tcgatatcga gcgcgcgacc gagacgcgtc cacgtcgtcg
    21121 cactgccggt cgccatcgct gcgagcacgc cgtaggagcc agcgtattga tcccatgcga
    21181 tcgcgatcga ggagccgcgc gccgcgagcg acagatgcgc gttggttccg acggtgagcg
    21241 tcgacatcgc cgtccacgcg ccgtcgaagc ggtagacgcg aagagcgccg gaatcgtcga
    21301 tccaaccaat cgcagcgcgg tcggcggccg atgcagcgag caccggttcg ctcgcgagcg
    21361 gcgcgggcat cgcgacatcc ataccgagcg gctgccacgc attaccgacg agcgtcgaga
    21421 cgcttgccgt cgagccgaag agcgcgagca tcggcgcgcc actcggtggc ttcgcgagcg
    21481 cgacgtatcg agcatcgccg ggcgagggca acgacgccca cgcgccgccg ctgaagcgcg
    21541 agacgaagac gctcccgaga tcgatatacg cgacgacgac gtcaccgttc tcgtcgagcg
    21601 cgatcgcact cgaggtcacg ttgctggcgc cgaacatgcc gaggtcgcgc cacgtcgtac
    21661 cgtcgaggac ggagacggcg agcttgcgcg cggcgagcgg gccgacggtc caagccgcat
    21721 agacgctgcc gtcggtgccg accgccagtt tcggcgatga cctcgcgatg ccgcgatcga
    21781 tcgggacgtt gctccacggc gcgacgacga acgcttcgtc ttcgtccacg gtggccgcat
    21841 tgccggccgc gtcttcgacg gaaccgccga gatgcaacga cagggagcca acgccacgcg
    21901 cgctcgtgtc gagcgtcacg cgaatcgtcg acggagcttc gaacgagacg ctcgcgccga
    21961 cgaccgagcc gtcgagctgc gccgatgcgg tcagcgccgc aacgctctgc gcatcgagcg
    22021 gctcgtcata gacaaagcgg accggcgcgc cgagccacgt cggggtgccg ttcgccggtg
    22081 tgacagagag caagtgcggc ggcgtcgtgt cggggacgct ggcgtcgggc gccgcatcgg
    22141 gcggggcgtc cgccgtagtg gcgtctgcgt ctcggccgtc gccaattgcg gcatcgagcg
    22201 catcgccagc gccggcgtcg agcgggcggt cgtgagtcgg cgtcgaacag gccgcgagga
    22261 gcgcacagac gagtggcgcc tgcgcgtggg aggtggaacg gcgcagcacg gcgtggtggc
    22321 atcgcaagcg atgtgccgcg aacggaccgt tccgtcggcg cggttagatg cggccgatgt
    22381 agcagcgtcg cccggcgagc cgcctcgcgc ggcctctctc tgtcaggtgc gctcggttgc
    22441 gacgtggctc acagcgtcga cgggacgtga ggcgccgggt agctcgacac cgaagctctt
    22501 cgccagcgcg agcagctgcg cgatcgcagc cggcaccgcc tcgaggggcc ccgttcccgc
    22561 gccattgccg atctgcgtgt agttcaccgt gccgatcgtc tgcgcgagcg cctcggcgat
    22621 cttcggcagg ccatgtgtga cgaggttcgt cacgacgcgg ccctgggcga cgaggttctc
    22681 gatctcggcg aggcggcgct gatgcgcagc ggagagctgc gcggcgtagt ctttctcctc
    22741 ggcgcgacgc cggtcgagct ccatgcgcat cgcttcgagc tgccgctctt gctcgagctg
    22801 cagggtcgac tggatgtgca gcgcctcgac gcgccctcgc tccgcctcga gcgacgcgac
    22861 ctcggctgcg gcctgcgcgc gacgatgctc ctccgcgatc gcgatctggt gcagcgctcg
    22921 ctctcgctcg agctcggacc tgcgccgcgc gagctctgct tcctgctcgg acttacggcg
    22981 cgcgagatcg gcttcttgct cggcgagctt tcgctcggac tcgccacgct gttcgacaac
    23041 ctgtcgctcg cgttggagct cggcgagctc ggcgcgtgtc gcaatctccg cgcgatatgg
    23101 agcttgaaga tgcgcgaaca cttgctggct ctgcacgcgc acctgctgga tctcgatcgt
    23161 gtcgatcacg acgccccagc ctttgttcgt cgtgtcctcc ggcgatccct cgccgccgac
    23221 cacgggcgcg atttcgtcca tgaggtagcc cgcgatcgcc tctttccgcc gcgtcaggca
    23281 gtcgtcgagc gcgagattcg caatgagccg gcgcgccgcg ccgacgaaca tctcgcgcat
    23341 cgtcgcggcc agcttctcgc tcgcgccgcc ctcttggaag ttgaggaccc gaaacgccag
    23401 cagcggcgcg gcaattcggt acacagcgat gcccgtcacc gttacgccga tgcgctcgcg
    23461 cgtcacctga tcggctgtga agtcgatgcg ctgcagtgtg gtcggcacga tcgacaccga
    23521 cagccacggc cacttgatga tgcgcatccc ctgaccggaa cgcttcgggt caatgcgacc
    23581 ttgccgcgta cacacgagat actcactggg tctagcgccg acatatacag ccatctcccg
    23641 acgaggtttg cagtggacgt gccgcttaca agtgggcagt tctccgtcga ggattgctcc
    23701 gtcttgaagc agcctgctct gttctagagc agcgaaaaaa caggtagcgc gacgaggcgg
    23761 cggtgatcat gcttttcgtc atgagcgcac cgtttggggt ggaggcgcgt tgtgaacgcg
    23821 cgagcatttc atctggcaag tcgacgttgt gggcatcgat tcgcatcgat cccaaaggca
    23881 aagcactcga gaacgatcgc gcaccgctcg cggtcgcact cgtgctcgac atctctggat
    23941 cgatggccgg cgatccgatc gcgcacgtaa tcgcagcgag ccagatcgtc tcgacgttgc
    24001 tggacgagcg agacaagctc gcgatcgtca cgttctcgaa tcacgccggc gtgcgctgcg
    24061 gcctgacgac gtgtgatgcg gacggacgca agctcatcgg cgatgcgctg cgcgcgatcc
    24121 agacgcaagg cggcacgaac atgcacgccg gcatggaagc agccgccggc attcttgcga
    24181 cggcgcccgc cgggctgcgc aagacaatgg tggtcctcag cgacggccag ccgaacgtcg
    24241 gcctgtcgag cgcggagcag ctcgcgcagt acacgacgag cctgcggccg atcggtgtct
    24301 cgacactcgg cttcggcctg catcatgacg agcacatcct gaccgcgatc tcgacggcgg
    24361 gctccggccg ctacgcgtac gttcccgatc ccatgatcgc gcgcgtcgat ctcgcgcgtg
    24421 cagcgctcgc tcatggcggc atcgtcgccg aaggcattca gctcgagatt cgtcccgcgg
    24481 agggcgtcga ggtgttgcgc atcatgcccg cggcgcagct gcgcgtcggt ggcagcggcc
    24541 tcaaggcatc gatcggcgac gtgttcatcg acgagtttcg cgtgttcgca ctcgagctcg
    24601 cgctcgacgt cgccacgacg ttccgcggca acctggccga gattcgcatc gagggacgct
    24661 cgcccgacgg caagctgcat caagtgccga ccgcgctgat cgtcgacgtg catggcggtc
    24721 cgcacacgat cattcgcgac gcccaacgcg acatcctgtt cgtccgcgcg atggccacgc
    24781 gtgtggaagc tcgcgcgcac gccgaccgtg gtgccacgcc ggccgccgcg tcactgctgc
    24841 gcgagatgat cgacgcgatc gacaagagcg aaggcttcgt gcgcgatgac gcatcgccgc
    24901 tcgccgagat gcgcgagcag ctgcaggacg aggtcgacaa ctacgagcgc aaggcatcgc
    24961 aggccgagca aggccatcgc tacaagatga tggcggcgta cacgccgacg ctcatgggcg
    25021 cgagcacgcc cgctcgcaag aagcagcgca acgactacgc gccgggattc ctcgtcggtc
    25081 tcagccacgg cgcgcagaat cgcaagtggc agctgttcac cgaaacgtcg atcggtcgct
    25141 cgcaggacaa cgagattcct ctatcggaag tgtcattgag ccggcgccac tgccgcatca
    25201 tgtacatgaa cggcaacttc gtcctcagtg acctcggctc gacgaacggt tgctcggtca
    25261 acggtcagcc cgtgtggtcg caggcttgga cgctgaaaga tggtgacatc gtgcaaatcg
    25321 gcgatgtcga gatgcggctc gagctgaaga agccgtagcc agacgcgagc gggttgggta
    25381 cggtcgcggg gtgactcggc tgtaccgccc cgcgctcgtc gctatcgcgc tcagcgcgtg
    25441 cgcgatgggc ggtccgggac cacgccgggg aaccgtcgac gccaacgacg acgagggtga
    25501 gctcgccgat gcgccgcgca cgtcgtcgac gcccgatgcg ccgccggcat cgagctgcgc
    25561 gcaggcattc accggtgtgc tcgcaacgtg gacgttgacg ggacaagcgg gcaatcaagc
    25621 gagcacgcct gcatcggcga cggcgccggg cgttaccgcg gctgctctga cccgttctgc
    25681 cggcctcaca gccgtcagtg gtgccgattc gatcaacgcg tcgggctggc cgacggcgaa
    25741 catggccgat ctcacgaagt actactcgtt tgcggtcaca ccgcccgcgg gttgcagcct
    25801 cgctatctcg tcggtgtcga tcgatctcgc acgctcggga accggccccg cgctgggcgc
    25861 gctcgcgacg agctccgaca actacgcgca acagattctc gtctcgacga acacggcttc
    25921 gacgccgagc ctcgtcgcgt ccggcggaac gatgcagctg cagattcgca tctacgggta
    25981 catggcgagc agcgcggcag gaacgctgcg cttggaaaac acactctcac tcacgggctc
    26041 gcttcactga tgacctgcaa acgctgcgga gccgaaacgt cgcgcgcgtg gtgcagggac
    26101 tgcgagcagc tctatgacgg ctggagccgg cggcacgcga gcgacatcgt gtggcaagca
    26161 ctcgccggtg gcgtcgtcgt cgcaaccgtc ggcatgggcc tgccgctgct cggcatcggt
    26221 tggctcgtcg ctgcgacggg cgcgttcgcc ggattcggca cgttgttcgg cctgtcgcgg
    26281 ctcactcatc gtcgacggcg ccgtcagttc ttgcagtcga cgctaccgcg cgcgtatctg
    26341 actgcgccaa agtagcgcgc atgtcaccga gctttctgta cggcacggcc tggaaagagg
    26401 acgacaccga gcgctgcgtg cgcgacgcac tcgccgccgg ctttcgcgcg atcgatacgg
    26461 cgaaccagcg caagcactac tacgaggaag gcgtcgggcg gggcgtcgca gcaagcggca
    26521 tcgcgcgcag tgcgctctgg ctgcagacga agttcacgtt catcgacggc caagaccaac
    26581 gtctgcccta cgatccgcgc gcaccgatcg cgacgcaggt cgcgcagtca ttcgcatcgt
    26641 cgtgcgagca cctccgcacc gactatctcg acagtctcgt gctgcatgga ccgtcgcaac
    26701 gcgaaggcct cggcgccgac gaccacgaag cgtggcgcgc gatggagacg atcgccgagt
    26761 cgggccgcgc tcgcgcgctc ggagtgagca acgtaacgcc acgccagatc gaagacctcg
    26821 tcgcattcgc gcgcgtcaaa ccgagctacg tgcagaaccg ctgctatgcg cgcgtcggct
    26881 gggatgcgct cgcgcgcaaa gtctgccgcg atcacggcat cacgtatcaa ggcttctcgc
    26941 tgctcaccgc gaatcgccag gtgctcgcga acaaggacgt cgctgcaatc gcatcgcggc
    27001 acggcaggac gatcgcgcag gtcgtgtttc gcttctgcca gcaggtcggc atgctgccgc
    27061 tcacgggcac gaccgatccg acgcacatgc gcgaagatct cgcgatggac ttcgagctgt
    27121 cgcccgccga tatcgcgacg ctcgacgccg ccggctagtt cgccgccagc gtctcgacca
    27181 ccgcgatgta gcgaacgctg cccgcgcatc cgcccatccc gaccgatagg ttctgcgcgc
    27241 ctgtatacgt cggcgtcggc gccgaggcca cggttccgtc gacgtcgagc gtgaaggtgg
    27301 ccgacgccat gcgcgcgatg aggtgcatga acgtcgtgcg gccaacggga aacgcgccgt
    27361 agacggcgtt attcagaccc gtgtagctcg tgccgtcgaa ttgcgtgatc accgcggcgg
    27421 cgccgctgcc cgttccgtcg aacagctcgc cgtagtagta gggcattgcc gcgtcgttct
    27481 gaatgatgat cgcgagctgc cgattcgttg gcgcgaggct cgtgagatcg attgccacga
    27541 agacatcgat gtccttgaag gcgccggcaa cgaacagatt cgagccgctg tttggcgaca
    27601 cgacgctcca ctcctcggcg cctcgcgtcc acgcttggcc cgtcgtgacg atccggctgt
    27661 cggggccggc gagcggcgag aagaacagcc aacgctgctg gctgacggcg ggctgcggat
    27721 cacaagcgtt gcctacgcga tcgaggtcgg tgtcgatctg cgttgggtcc ggatagtgcg
    27781 ggcacacatc gatcaagtcg ggaacgccgt cgccgtcctc gtcgtgacca ctcagagcat
    27841 cgacagagct cacgtcgtcg tttgccgcgt cgccggtcgc gtcgaacgac agccgcccgc
    27901 accccgaaag caaaacgacc ggcagcgccc accgcatgcc ggtccagtat cgatcgtttg
    27961 tgcgcgctag aagtagtaac tcgcgccgat cgtcgtctgg ccgcccttcc aaccgtcggc
    28021 ggcgggcgag tacgtcggat ctttcggcgg gggcgtcggc tccatcgtct cgccaggcgc
    28081 agtcggcacg gcaccgccgc cgtcgtacac cgtcatgtcg tcctcgttcg gcgcgacgcc
    28141 gacagcgcga atctcgagct gcagcgcgaa ccggtgccag cgacgctcga gaccgacgcc
    28201 gaactgcagc gtcgattgca cggcggcttc cttctcgtca tcggacgccc attggttcgt
    28261 cactgcgaac gagcccatgc cggccatgag ccaccagttc cagcgcttct gcgggttgaa
    28321 gcgatagcga agcgcgagca cgctcgtcga gacttcgcgc tcaccttcgg tgccgtcttc
    28381 gagttgttcg cgaccgccgc cgagcgcgag ctcgatctcg aggtgacgcc acggccgata
    28441 gcgaagcgcg agctggccga cgccgaactc gctctcgttc tcgggctgcg tgtgcggcgc
    28501 gacgttgagc ccgccgaagt tgagaccgac ggaccagcga cgctggcgca ctggcggagg
    28561 tgcgggcgcg ggatccggcg cgacttccgg cgccatgccc ggaggcgcga gcgtttcgtc
    28621 accttcgtag tagtcgcctg gagcctgcgc gtacgcagcc gacgacaaac cgcagagcac
    28681 cagcgaaccg agaaccagcc cgatcttcat ggtgcttcta ttctgcgcgg gtcgtgccac
    28741 ggtaagccac tgaaatccgg cgtcttcgat cgtcgataga aacgacacct ggcggcgcga
    28801 tcgtcgcggc ttcctacact acgatgggca cgtgccgggt cggaccttcg ccataggcga
    28861 catccacggt gatctcgtgg ccttagccac tctcttcgat cgcctgccac ccctcgtggc
    28921 ggaggacacg gtcgtgttcc tcggcgacta catcgaccga ggtccggact cggcgggagt
    28981 tgtccgctgg gtgcgagagc tgggcaagcg gatccccgcg aaggtcgtct gcctgcgcgg
    29041 caatcacgag gacgcgtggc tgcaagtcgc cgatgaaggc tggccggagt tcatcctgcc
    29101 gcgcggcaat ggctgcctcg aatgctatcg ctcgttcgcg aatcttgccg ttcccgacga
    29161 ggaagaggcg ccgacgccag aagagttcga gcagatgctc ggcgcgaagt tcttcccgcc
    29221 cgacgtcctc gagtggatga ggtcgctgcc gttcttctac gaggacgagc acgcgatcta
    29281 cgtgcacgcg ggcatcaagc gcgaaggcga gggcttcccg catccgtcgg cgacggagcc
    29341 gaagcgcgcg ctgctgtggc ttcgcgatcg cgatttcttc gagaactacc gcggcaagat
    29401 cgtcgtgttc ggtcacacga ccacgcgcac gttgccgaac gagctctcga catacacgcc
    29461 cgacgatccg acggacttgt gggcaggccc tgcgtgcgtc ggcatcgaca cggccgcggg
    29521 caagggcggc ttcctcaccg ccttcgagct gcccgcgcag cgagtctacg aatcgcgtga
    29581 ctaagacgcg gagcaccggc gccccaggct cggatgcggt tgcgttccgc atcggtcgcg
    29641 cgctcgcatc tcccgcgggc gtgctgatca cggtgccggt tctcgtgatc gcggttggca
    29701 tcggcatcat gttgctcggg cgcaacgcga cgcgaacctc gacgcattcg atggcgcggc
    29761 atcagctcgt cgaacaggcg cagagcgtcc aatccgatgt tgcgtacgcg ctcgaccaag
    29821 ccgatccgct gatcaagctc gtcagcgtgc tcgcggtgcg cgaccgcccg ctcgacgaca
    29881 cgctcgttcg cttgcacgac ctgctcgcgg cgcgaccggg catcgcattt ctcagcatca
    29941 gctttcccga cggcacattt cgcggcgcgc agtacaacgc ctcacgccag atcgaagtcc
    30001 aggagagcct cgtgtctccg tcggaggcgc gctggttctc ggtcgtcgac ggcaagctgc
    30061 agctgctgcg aaccgagccg caccaatacg atccgcgcaa acgtggcttc taccagctcg
    30121 cggtcgcgaa gcgcgcacgc gcgtggaccg agccgtacac gttctttcgc agtcacgaga
    30181 ccggcatcac gtgcaccgag ccgctgttcg acgcgatggg caatctgcaa gcggtactca
    30241 ccgtcgattt cgacgtcggg gcgctgtcga gcttcgtcgc gcggccgggt ctcgatcagg
    30301 cgcgcacgct cgtgttcacc gccgacggtg tgatcctcgc gtatcccgcc gccgaccggc
    30361 tcggcctgcc gacaagcgac aagctgctgc gcgccgaaga tcttcgcgac ccggcgatcg
    30421 acgcgctgtt cgccgcaaag ccgacgtcca cgtcgcgctc gcagcgcttt ctcgatctcg
    30481 atgcgcgtga cggcgagtac ctcgcatcgg ttgcaccgat cggcggcaag cgcgcaggca
    30541 tcgaggtgcc gctcgattgg tatgtcgcga cagtcgtgcc cgctcgcacg ctgctcggtc
    30601 caacacgcaa gctcgagcgc gcatcgatca tcgcaagtgg tggcgcgatg ggcatcgcgg
    30661 tgggcctcgc gctgatcctc gcgtggaacc tcgtgcgcat gcgccggcag gtgcagagtg
    30721 cgcgcgaaga agctcgctcc gccgaagcgc gcgcccgtga gctcggtagc tatcggctcg
    30781 tcgcgaagct cggctcgggc ggcatgggcg aagtctggcg cgccgagcac cgcctgcttg
    30841 ctcgctccgc tgcgatcaag ctgattcgcc ccgacgtgct cgactccgac ggcatctcgg
    30901 agatccgcga gcgcttccgc cgcgaagccc aagtgctcgc atcgatgaag tcgcgccaca
    30961 cgatcgcgat cttcgactac ggcgtcacga acgacagcac gttctattac gtgatggagt
    31021 tgctcgacgg tctcgacctc gaatcgctca tcgttcgcta cggcgcgcag ccagccgctc
    31081 gcgtgatcga gatgctgatc cagtcctgcg cgtcgctcgc cgaagcgcac gaccacggcc
    31141 tctaccaccg cgacatcaag ccaccgaacc tctacctctg ccgcgcggcc gacgaggtcg
    31201 acatcatcaa gctgctcgac ttcggcatcg tgcaaacggt gaacgagctc ccgcgtccgg
    31261 tgacgcgcct ctccgctgca cccgctctcg agacgccgaa gctcacgcag atcggcgcga
    31321 tgctcgggac gccgggcttc atggcgcccg aacaaatcct cggcatgcag atcgacggtc
    31381 gcgccgacat ctactcgctc ggctgcgtcg catggtggct gctcaccggc aacgaaatct
    31441 tcacgcgcga aggcggcgag gcgaaggtcc tgcaccgtca catctacgag gagctcccgc
    31501 cgctgcgcga gcgcgtgcgt ggctggatcc cgccggagct cgaagcggtc gtccacgcgt
    31561 gcctcgcaaa agaagccgac gaccgcccgc gcgatgcgcg cgagctcgcg aaacgcctcc
    31621 gcgcgatccc gatccccgac gactacgtgt ggacggccga acgcgcgcaa gcgtggtggc
    31681 gcagctacag cccgccggcg gccgtgccaa acctcccgtc gggcgaggtc caggtcatcg
    31741 ttccgggtcg cccgtcgacg cacctaccga tcgcagcgac caagaaggac gcggtcgtga
    31801 ccatcgcggc gccgaccgcg agcacgaagc tcgacatcgg ccgcaagtaa tcagagcgag
    31861 tcgaccgacg cttggatcgc ggcttggacc accttgccga agcgctcgcg ggacgcgccg
    31921 tcgaactgcg tcgtgtacgg caggtggatg aagccggcca cgccgcgcga cgacatcgcg
    31981 ccgacgtcgg cgaacatgac gttgttgcag atgtagcgac cggggtcgcg gctgtgctgc
    32041 ggcgtttcgc cgagcgcttg caacgcttct tcgatgcgat cgagcggcag cagcgtgttg
    32101 cgcgtcgacg gtgcggcggg atcgatttgc gcggcagcgc ggatgatgcc gcggttgtcg
    32161 ggcacgccgc cggcgacttc gccggtgtcc tgcaggttgt acgcgacttc ttcgagcgcg
    32221 atttcgccgc caccttggcc gaacgagatc acgacgcggg gcgagcagcg agtgatgaga
    32281 tcggtgactt ccgcggcggc gcggtcgtac tcgaccggca tcacgacgcg catgacttga
    32341 gcattgcgca ggcgtgacgg atcgagcgcg gtgactgcga cggcagagac gttctcgtgc
    32401 cagccgtcgg ccgggaatgg ttggaagccg gtgacgagga ctttgacgcc gttcggatcg
    32461 ccttcgaact tgcaggcggg cggggtccgg cgaagttgga cgcgagcggc gagcgcgccg
    32521 gcgcggagct gaagctcgga ggcgtcgacg cgctcgagcg cgagatcgcc gacggtgagc
    32581 tcgatgagcg tgcggtatgc ggtggtcgtg cgcttcgctt tgccggccgc gatgaggtcg
    32641 gagatgcgcg tcgtgatgtc tttgccgtcg agcgtgacgc gatcgagctt gtgactgcgc
    32701 ggcagctcga acacgagctt gtcgctgtcg acgaacgagc cggagcctgg cgaggcgacg
    32761 aggccgaagc gctcgttgaa gacttcgatg taatcgagat cgatgaccgc gccgggtgcg
    32821 ggtgtgactt tgacggtgac cggctggcct tcggagctcc agctgagcgg gaggtcgacc
    32881 cacgatgcgg atcgtaagtt tgtcgtcgtc agcgacgagg acgcgaggat ctcgccatcg
    32941 acgccgagga ctgcggcgga gacgatcgtg ccggatgccg cgtgagcttt gaccgagagg
    33001 cgcaccgatg cgacgaggtc gccgtgctgc tcggagccgc cgagcgtgcc ttggcaggct
    33061 ttgtccttga ggcgaatgct accgttgtgc ggcacaccgg acgatgcgca cagcgactcg
    33121 gcttcggtaa cgcgcgcgtt gaccggatgg cccgcgctgt cgaacttgcc gtcgagaaag
    33181 tcgcgaagga ggccggaatt cgggtcatcc gttgccggca tgtcatccgc gcccggcggt
    33241 tccacgcaac cggcgagcag cgagctcgtt acgacggccg cgaggccgaa gagggcggcg
    33301 gtgatgcagc caccgtacgg agagtgcgag ccgggcatgg cctacggata gagcaccctg
    33361 cgtgccacga cgctgccgtg gcgggacggc ctcttgacgc acctgctgct ggaagctcga
    33421 gtagccatgg cagccgatcc gtacgcatcc tgtggttaca ggctgagcgt tcctcgaccg
    33481 agctcgaccg ccgaaccggt cacgtagatg ttgcgatcga cgtcgacgtg gaggccgagt
    33541 ctcgacggcc ggccgaccgc agcaccttgc gagagtgtct cccgcacggg cagcgatttt
    33601 ccagtggcga gcatccagcc gccgaggttc gcgcatgccg agcccgttgc cggatcctcg
    33661 atcaccgcgc cgtgctgcgt gaagaagaag cgaacgagca gctcgtcgcc gtcgcgcgtc
    33721 cagagatacg ccatcgcttc atcgcgcgtt gccgagaatc cgtgcttcgc gatcagctcc
    33781 ggcgcgggcc gcgcggcgcg aacgtgatcg ggcgacgcga tcggaatcac cagctgctcg
    33841 acgccggtgt tgacccacag cggctcctcg ctcacggcgt ctgcgggaag cccgaccatc
    33901 gcggcgagct cggcgcgtga cgcatcgacc ttgcgagtct ctggcgcttt cgccgcgcgc
    33961 agcgtccacg catcgccgct cgcgctgacc tcgacgatgc ccgccttcat ctcgagcgtg
    34021 acgcgatcac cgccgcgcat ggcgcgtacg acgtgcgcgg tcccgagcgt cgggtggccg
    34081 gcgaatggca tctcgaaacc gggcgtgaag atccggacgt gagcggtcgc gcggtcggtt
    34141 ggcagcacga acgtcgtctc cgacaggttc atttggcgcg cgagtgcctg catctgctcg
    34201 gtcgatagac cgcgcgcgtc ctcgaacacg cacaagggat ttccggacag ccgatcaccg
    34261 tcgacggtga acacgttgat gatgcggaac gcgagcgaca tgccttggtg tacgtgcgac
    34321 ggcgaccacg tcgcaactac acatcttggt tatcgaggcc gatacagctt cgccttccgc
    34381 tagactacgt tcgtgcccgc gactgtcgtc gacgcggtga agacgccgta cccgtgcgcc
    34441 tgtcgctgcc acgagctgct ctccttcgaa gagagagtcg ccggaatcgc agcgctctat
    34501 cggatcgatg atgcgatgcg tggctggggc cagaccgtga tctgggatct cgcggcaccg
    34561 acgttgtggc gcgtgcagca acagctcggc gaagtgcgct gggtgacggt gcgcgacggc
    34621 ccatgcatcc acagccgctt gctcggattc tgcgtgcacg agctcattca tgcgatgtgc
    34681 ggcgatccga acgcgccgaa ctggggcacg cccgtcggcc ttccatacgg tgttcccgag
    34741 agtgttcctc cgacggagga agctgcgttt ctgcatccgt tcaaccagca cgaagcgcgc
    34801 gcgtgggtcg gcctcggcgt cgtcgcggag cgcttgttcg ggatcgagtg gacgctgctc
    34861 cctgcgcgcg acgtcggcac ctacggcttt cccggcggca acgcgctcgt cgacgttccc
    34921 gaaggctacc gtcgtgtgcc gcacttcgat cacaagctcc acacgcgtcg ctacctcgcg
    34981 ctcgcgcgca agctcgaaga agaagcgctc gcgtggttca cgaccgagaa gctcgacgag
    35041 atcgcgcaac gcttcgacgc cgccgagaag ctcggtcgtg cgcaacgtcc gttcgagttt
    35101 ccgtcgccga aagagatggc gcggctcaag ccgaagcagc ccgggcgcaa cgacctgtgc
    35161 gtctgcgggt cgatgcgaaa atggaagcag tgctgcggcg cgaacgtcgc ctaaaagcag
    35221 gcgggcgttc cgggaccgct gctgcagcag acgtcgccgc acgtcgagct cggcggattc
    35281 gtcatcgccg ctgagacgca gaacgtgtcg tccgcgcagt gcgtgccgtc gccgcagcga
    35341 cattcaccgt cgacacaacg ctcgccacgt ccgcagcatc gcgcgccgca cgcggccgcc
    35401 gagtccggac actgcgctgc tgtcgcgata cacgtcgtcg agtccatggc cggcttcgcg
    35461 catagcagca tcgtgtcgtt gggatcaaac tgcatgactc tgcacgttga ctgcgactcg
    35521 cacgcgagct cggacggacg cacgagcaga ggaacgagca tgtgcttacc gttgtcgagc
    35581 gccacgtcca acagcgcgga gtgcccctgg agcgtccgca tgtagtcgtc gaaccacttc
    35641 acgtcgacct tgcagttttg gaagttggcg agctcgaacg tcgccccacc gacgatgatg
    35701 tgatcgtcaa acggcccgag cggcaacatc agctcgtgca ccttgatcga cgtgcacttc
    35761 gtcccgccgc cgccgctgat gaacatgccg agatcgatct ccgcatcgcg cttggccgaa
    35821 tcggggcatt cggtcccgtt ggctccgacc gccatcagac agttgacgaa cggaaccgtc
    35881 ggcatgttcc tcggatcgat ttgtgtcgtg cacgtgttcc agtcgaagca accacacgcc
    35941 ttgctcggca tgcagtagtc gacatcgagc gggtcacaat tcaccgacgg caagccgcca
    36001 tccgtgcacg cggggccgcc gaggaggcag acctgtagcg gtccggtcgc gttgaacacc
    36061 ggcgtctgaa tcgggatgac gcagttggcc tgctcgagcc gcggcgcagc gtgcatcgcg
    36121 ttcggtacgt acggagcgca ctcgtgggac atcaccccgt cgcagtccgg atcgtcgacg
    36181 ggcaccacac catcggcgcg gccgtctccg ttcacaacga gcacgcagcg aggcccggtg
    36241 cggttcgcac cccagatggc gatgcgctct tcatcggacg gagcatcggt gagtggagac
    36301 acgtgctcgt cgagcaacac ctgccagtaa tcagcattcg atgccggaat cgtcacgccg
    36361 gcgaagcggt tcaacgcgat cggcgttcgc gctgcgtcga agccgatgat caacagatac
    36421 ggcaggaagt agtcatcggt gtcctgcgaa gttatgcgaa agaacgcgtg gccgttcttc
    36481 cagttgaccg cgtcggcttt gctcgccgaa aagacctgcg cgtagtcggt ctcgaacatg
    36541 ttcgcggtgt gcgcgcgcat catcggcggc gtcatcgcat ccggacaatc gtcacagtgg
    36601 tcggcgagat agacctcgac gtactcgatg ctcgggtcga gcgggaccac ttcgagcgtt
    36661 agcccgggcg gctcgttgca ccccagcaac aagagaagcg ctccaaccac cgctcgcacg
    36721 cttcgatcat aagcgatatc gtccccggca aatgcgtttc gtcgccattg cggttctcgt
    36781 tgcttgctcc gcacccgcgc cgagacgggt tcccgtcacg ccggggctcc cacagccggc
    36841 ccagcccaca cagccggcgc agccaccggc cgacgcaccc gtcagcggcg acctcggtcc
    36901 tgcgaagcca acgctgcggc ttcccaagaa cttcgtcgcg aagtcctaca ccgcgtcgct
    36961 gtcgatcaat cccgcgcagc cggggttcgc aggcgcgatc cagatcacgg gcgaggtgag
    37021 cgcgaggtcg tcggtgatct ggctgcatgg ccgcaacttg acgatcaaga aggcgaccgc
    37081 gagccgcgat ggcgccgcac cgacggagct ggtcgtgaag caagccgatc aagatttgct
    37141 cgagctgcgc agcgcggccc cgatcgagcc tggcgcctac gtgatctcga tcgactacgc
    37201 cggcacgttc gcgaccgtcg agaccgctgg cgcattcgtg caggtgtccg gcaagtcgcg
    37261 ctacgtcatc acgcagttcg aagcgctgtt cgcgcggctc gtgtttccgt gcatcgacga
    37321 gccggactcc aaggtgccgt ggcagctcac gctcgacgtc ccggcgaagc aggtcgctgt
    37381 ttcgaataca ccgatcgcga aagagagcgc ccttcccgac ggccgccgcc atgtcgagtt
    37441 cgagcgcacg ctgccgctcc ccgcgtacct cgtcgcattt gcggtgggtc cgttcgacgt
    37501 cgtcgacgca gggaagacga agcgcggcat tccgattcgc attctcgcgc tgcagggccg
    37561 tgcgaaagat gccgcgtatg cggcctcggt aacggcgcgc atcgccgaca tggcagaggc
    37621 ctggttcgag atcccgtatc cgtacgcaaa gctcgacatg gtcacgatcc cgatcagcgt
    37681 cgggttctcg gcgatggaga acgcgggaat gatcacggtg accgagagcg agatgctgtt
    37741 cgatccgcag aagatttcgt gggcgaacaa gcatgcctac gtctcgacgg cggcacacga
    37801 gatcgcgcat cagtggttcg gcgacctcgt cacgatggtc tggtgggacg acatctggct
    37861 caacgaaggc ttcgcatcgt ggctcgagaa caagttgacc gcgcagttcg agccggcgtg
    37921 gcacgaagag ctctccgacc tcgacacgcg cgctcgagcg ctcggcgcgg acagcatcgt
    37981 gacggcgcgc cgcgtgcgcg agccgatcgt cactgaggac gacatcgcga acgtcttcga
    38041 ttggatcacg tatctgaagg gcgccaccgt cctcaacatg ttcgaggcct acgtcggtgc
    38101 ggagaaattc cagcgcggcg tgcgcgagta catcaagacg cgcgcgatgg gcaacgccac
    38161 gtcgacggac tttatcgcgg cgatcagcaa ggcaggcggc gtcgacgtgt cggcgggctt
    38221 ctcgtcgttc ctcgatcagg gcggcgcacc ggagatcgaa ttcgcggtga cctgtggcaa
    38281 gggcaagccg cgcgtggagc tcgcgcagca gcgctacgtc gggccaggct cgccggcgcc
    38341 agaagccaac aagccgtgga tcgtgccggt gtgcatcgcg ttcgacaacg cgggcaaacg
    38401 cggcgaagcg tgcacgctga tgtcgacgga gacgacgtcg ctcgagctcg atgcgaaggc
    38461 atgtccccgc tgggtggccg gcaatgcgaa cggccgcggc atgtaccgca cgcgctacac
    38521 gctcgcgcag gcgacggcac ttcgcgatga agcctggccg cagctgacgt ggacagagcg
    38581 gcgcgcagtg ttcgcggcgg tcaagaccgg gaccgaaggc gtgccgcgga cgatgaagaa
    38641 gtcggtgagc aaaaagccgc cggcgaagct gccgctcgcg ctcgcactgt cgttcgtgcc
    38701 gcgcatgctc gcgggcggtg atcgcttcac gatcggcgac gcaaccgacc tcgccggcgg
    38761 tttcgattgg gacgtccccg aggggcagcg cgcgaaatac gagagctgga ttcgcgcgac
    38821 gttcggcgcg ggcgccgcga aggtcgggct cgtcgcgaaa cccgacgacg atctcgatgc
    38881 ggagacggtt cgcaagaagc tgctcgggct cgtcgcgtgg accggacgcg atccggatct
    38941 gaccaagcag gcgacggatc tcgcggcggg ctggcgtgac atggcgtcgg cgacgcgcgg
    39001 cctcgtgctg aagatcggcg cagactcgac gccggacctg cacgcgaagc tgatgcgcga
    39061 catctatacc gagcaggacc gggcgcgccg cggcgagatg tattacgcgc tcgccggtgt
    39121 ccgcgatgcg aagcgatacg aagctgcgct cgagctgact ctcgatccga agctcgattt
    39181 ccgcgagtcc gacacgatca tcgtcgcgtt ctcgaccgag ccgatgcgcc aggtagcgga
    39241 gcgcttcaca cgcgtgcacg cggacgcgct catgcagagg atgccgaagg agtccgcgac
    39301 gggcgagggc cggatcttca ttcgcgtagc gacacgcagc tgcgacgaat ccaagcacga
    39361 cgagatcgag aagttcctcc gcggcacgat tggcaagctt cccggtggca agcgctcgat
    39421 cgaccaaggc atggaagagc tcgaccagtg catcgcctat cgcaaggtga tcgtgccgga
    39481 gctcgaggcc tggctatccg gcgtgaagct tcccaagccc gcgaaaccct agccggctga
    39541 gcgccatgta accgccgcgg gcgagagcgt cactgttctc gtatgcgtgg tgttctggct
    39601 tttgcggtcc tcggtatcag ctccacagcg tcggcgcagt cgatcgcgat cgacgagttt
    39661 cgtcccgcga tggacgcgcg cgggtatctg acgctcaacg gctcgcagac cctcggtcac
    39721 accgagatgt cgttcggtct cggctcgctc gagtggggaa agaatctgat gtccgaggac
    39781 gtcggacccg gcgccgtcga caacatggtg tccgcgacgc tcgttgccgc gctcggcctt
    39841 catgccggac cggttccgct cgagctcggc gcatcgttgc cgttctcgat cgtcgatggc
    39901 tcggtcgatg ggcaaggcat cggcgacttc ggcttccatg cgaaggcgcg cgtcgctcgc
    39961 gtggggcgcg tcggtgttgg cgcgatcgcg agc
//
LOCUS       DATLBZ010000019         9809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8958354, whole genome shotgun sequence.
ACCESSION   DATLBZ010000019 DATLBZ010000000
VERSION     DATLBZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8958354"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VMZ53_00810"
     CDS             complement(<1..1202)
                     /locus_tag="VMZ53_00810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27008.1"
                     /translation="MSDSFAAGLVGVVLDERYRLDALLGEGGMGAVYRAHHLQMDRRV
                     AIKLLKPHLTSDDAQVQRFMREARATMKVDSEHAVKVLDFGITPQRDYYMALEYLDGR
                     TVQRELDVDGPFAPARVIHIAKQSLHALGAAHAAGLIHRDIKPDNILLMRVGNDLDYA
                     KVLDFGVAKLLAGTARSDRSAMAITQAGMVFGTPEYMSPEQACGQELDGRSDLYSLAA
                     TMFAMLTGCGMYNAKTAIEWLTHHARTPPPHLADGSKELAAYPALDKVMQRCLAKRRE
                     ERPQTAAEMIALLDSVAPMLGAAKSAASSSARGLAMRAAQSSYFEAIDPNATLAPGAA
                     ASVGGIAATPESPYAKTMMPGSMQRPAISDAYAHTMMSGSMSRVSTTPGTSLPAMPST
                     TTGVIAEVR"
     gene            complement(1215..1409)
                     /locus_tag="VMZ53_00815"
     CDS             complement(1215..1409)
                     /locus_tag="VMZ53_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27009.1"
                     /translation="MSNALVPRNKAVAKRKGTFAAAAAAGAGVAAIAGAPVIAVIGVA
                     GAAYLAWDWFSFRVKNGMRF"
     gene            1521..2936
                     /locus_tag="VMZ53_00820"
     CDS             1521..2936
                     /locus_tag="VMZ53_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HUS27010.1"
                     /translation="MSNRLGQRGTGNPAILLGLAGTMLVAAGGLAGGYYVVSTRSEAR
                     AATRPEPRSSDKQQKPVATNAKPTPALEALLKKTVDVKTGSAIAKLTWADLGVEIDPG
                     ELGRSSVASDAELNALATKGSIPVRLDRDKAIKALLQLKGQYDRGAIDAYLDLEERVI
                     HDDSPGQGIDVWASLQRIEAGARQAAPAIELVAIGVPARVTKQGLGIDDISNVMGVYE
                     TKFAVADKDRNFNLKLAASHINGAVLKPGEEWSFNGTVGERSQATGYKVAHVITAGEM
                     VDGLAGGTCQISTTLFGASFFAGLEIVKTTNHSRPSAYTPLGFDATVVWPNTDLVLKN
                     PYDFPVAVLYRVANGIAHVEILGKKRPYTKVEFAREVLEETPYNTEERLDEEMPEGLT
                     MLDQQGFNGYKLKRYRRFYQGKKMVKENKWTVQYKPVTEYVRRGTNPDPATPVPKEKD
                     LHPLKAPKPEDFNKERVVLTQ"
     gene            complement(3032..3781)
                     /locus_tag="VMZ53_00825"
     CDS             complement(3032..3781)
                     /locus_tag="VMZ53_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HUS27011.1"
                     /translation="MRLLYDLPSVFAAQIAQAEYESGRVYEPMIAIVTDNKDPQKLGR
                     VKVKYPNLSDQDTSWWAPIIMLGASKNRGWFFIPEKDDEVLVLFEHGDLNRPLVVGAL
                     WNGKDKPADKNPGGNPRRIIKSRQGSKITFDDDKNQLIIEDGGGIGIITFDAGSNKIT
                     IEAKQGDVCFQAPKGEIKVVAKSAEIKGGSNLEIHSGKATAWGSDAGVKINGSGGVTM
                     SGSKVNLNCGVAQAPSAPSASPKDVEDPYGS"
     gene            complement(3885..6755)
                     /locus_tag="VMZ53_00830"
     CDS             complement(3885..6755)
                     /locus_tag="VMZ53_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUS27012.1"
                     /translation="MRADVGRIVIVAAGGALAFAPVEYALTLRAYAGHIEAMSKLRLV
                     ALTLTLALWLWLMLAVALVGATLVWRLWRTVFDPVDARREGWFVPAPLDEHGVRHGVP
                     RAWALFATLGVVGLIIQHRSVWLTGAYQEVQLRGIYIARDALVAVAIGVPLHRLFSIA
                     TRVAARALAPVLGVVNPFGRWRAAGVAWTGVVAAALIAIWYVLPQSRSVLPVRLVTAG
                     CVIALGMGMGAYFAPRFAARPRKRTTALAVAGATLALCVLSLWRWGADVETKYVALTA
                     SPPLANLIKVVRTANDLDRDGFGTVLGENDCNPLKPSIHPGAQDLPDDNIDQNCDGRD
                     FSLKPSAIATGPAMPVPDQFRRSDWNILFITIDTVRYDHTSFGGYKESTKARDTTPRL
                     AELAKRSTSFTFCNAPSAGTMASIPAIMTSKFFHSGIALDENRPAGTPPGIKPENTTL
                     PEIMKRAGYHTGVISSHHWWNDWGFEQGVDEYDNSIGKSTDPFIVAADRVTDHILAWV
                     SRQQGHRWFLWAHYIDPHGRYVAHPDVVDYGSSEPDLYDAELRWTDQQVGRLLDELKR
                     LPSDDHTIIIITSDHGESMGEHSVPVGTHGTALYRELLHVPLVFYIPNLPPKQIGGAV
                     TNLDVIPTIAQITGIDVKDLTFEGKSLVPQLFYGKEDHDRIVFAETNAPSPIRAAISD
                     SWKLVYYLQSNLYELYDLQADPLEKTNLAPKSPPAMQLMKSALDAWLERVVYARDATF
                     NQADKKMAELVLPAAPTPEVTTQGQTLDDAKLEVLGISVDQGGSLPAPNAHVDINVFF
                     HVKARTQVAYKFLLAAWPVNTATWKPTDPAPSDLAKSTMRPSGEGFFASDRWRAGEYI
                     RDKFSVTIPPGWTGDGLAVGLVASDPKSEKALATGAAPSNDPNLIVLGVLPFRGTAPT
                     PQPQPQPPPPPQPQLQPQPQPPQNPSPTIQPPGLPGPTQPRP"
     gene            6871..8397
                     /locus_tag="VMZ53_00835"
     CDS             6871..8397
                     /locus_tag="VMZ53_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27013.1"
                     /translation="MPDARPPQRQRLRQLGWLVAIAVAYLYIFPYFPRIQSANELPRA
                     YLVKAIADDHTFAIDKGVATWGGTADVSPSDGHMYSNKAPGSSLVVVPFYWAVSKIAG
                     PPSLATTVWMCRVFGGVIPALLFLWLLWGFLAQYAPDPNVRRLVLIAYALGSMAMTYA
                     LLYYSHQLSAVCIGSAWILGLDVANGRRRVAWMAAVGFLAGASVLVDYQAAFAGVPLL
                     VYLIVRMWQRDASQILPALGIAAATAAIPIALLLAYHDVCFGGPLRTGYDASTTFAHF
                     HKQGFLGISKLRGEAFWGSLVKADNGLLVLSPWLLIAIPGGIYLWRRGERGHVLAVIA
                     IVVIYVAFISSIAMWRGGWGVGPRYITVMLPFLLPLVATALEKWRTQDLLFGAVSGTI
                     VAAVAIYSLSSVTFPYWPDSLKNPLYEVTFRLLHDNAVAPNLGSALGIRGVAGIVPYL
                     LCVFGLLAYTLYRAGNVRVLAVAAAVGLAILVAFRFAPLTGPHADVAYKNTVFPAVTK
                     "
     gene            8415..9353
                     /locus_tag="VMZ53_00840"
     CDS             8415..9353
                     /locus_tag="VMZ53_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS27014.1"
                     /translation="MRAWVPLLLLAALIGGCKDKKRASTYEDAGTGSNAIDPSELAAR
                     YDKKCVAGDLEACRALGVMYTEGTGVSPDPRRAAALFGQACAGDNLSACNHLALALSE
                     GMGVTKDVARAVDVYQKACDRGFVLSCRNLGLMLRDGRGVDVDLARADKLLSTACKGK
                     IPFACTNAGDLDSLLAAKQPAGSTAQKQHAAAAIAHYRLGCDAGDPTACRQIGIAYLE
                     ARGLPRSTAAAAVWLERACVGDDAIACRVLGAMNMEGVGVAKDTKRGQELLQRACDRK
                     DDEACRLVKLGAAAGSNAAAAGSNAAAVGSGSAASQ"
BASE COUNT         1603 a         3188 c         3285 g         1733 t
ORIGIN      
        1 cggacctccg cgatcacgcc cgtcgtcgtc gacggcatcg ccggcaggct ggtgccaggg
       61 gtcgtcgaaa cgcgcgacat cgagccagac atcatcgtgt gcgcgtatgc gtccgagatc
      121 gccgggcgct gcatcgagcc gggcatcatg gtcttcgcgt atggcgactc gggcgtcgcg
      181 gcgatgccgc ccaccgaggc tgcggcgccg ggtgcgagcg tcgcgttcgg atcgatcgcc
      241 tcgaagtagc tcgactgcgc ggcacgcatc gcgagacctc gcgcgctcga gctcgctgca
      301 ctcttggccg caccgagcat tggcgcgacc gaatcgagca gcgcgatcat ctcggccgcg
      361 gtttgcgggc gctcttcgcg gcgcttcgcg aggcagcgct gcatcacttt atcgagcgcg
      421 ggataggcgg cgagctcttt agagccgtcg gcgagatgcg gcggcggcgt gcgcgcgtga
      481 tgcgtgagcc actcgatcgc cgtcttcgcg ttgtacatgc cgcagccggt cagcatcgcg
      541 aacatcgtgg cggcgagcga gtagaggtcg ctgcggccgt cgagctcttg cccgcacgct
      601 tgctctggcg acatgtactc gggcgttccg aacaccatgc ccgcttgcgt gatcgccatc
      661 gcgctgcgat ccgatcgcgc cgttcccgcg agcagcttcg cgacaccgaa gtcgagcacc
      721 ttcgcgtaat cgagatcgtt gccgacgcgc atgagtaaaa tattgtcggg cttgatgtcg
      781 cgatggatga gcccggctgc atgtgcggcg ccgagagcgt gcagcgattg cttcgcgatg
      841 tgaatgacgc gcgcgggtgc gaacgggcca tcgacatcga gctcgcgctg caccgtgcgg
      901 ccatcgagat actcgagcgc catgtagtag tcgcgctgcg gtgtgatgcc gaagtcgagg
      961 accttcacgg cgtgctcgga gtcgaccttc atcgtggcgc gcgcttcgcg catgaagcgc
     1021 tggacctgcg cgtcgtcgct tgtcaggtgc ggcttgagca gcttgatcgc gacgcggcga
     1081 tccatctgga ggtgatgcgc gcgatagacc gcgcccatgc cgccttcgcc gaggagcgca
     1141 tcgaggcgat acctctcgtc gaggacgacg cctaccagcc cggccgcaaa gctgtctgac
     1201 atgtcggcag cgagctagaa gcgcatgccg ttcttgacgc ggaacgagaa ccagtcccac
     1261 gccaagtacg cagcacccgc aacaccaatg acggcgatca caggcgcacc agctatcgcc
     1321 gcgacacctg cacccgcagc cgccgcagct gcgaaagttc ccttgcgctt tgcgacagcc
     1381 ttgttgcgcg gcacgagggc gttactcacc tccggaggct gccttatgga ataccccctg
     1441 tcaaaccggc gtttctctag acgctcccca catgggtggg aaatagggat gtcgtcggga
     1501 gcgtgataaa aaggagctag atgtcgaatc gtctcggaca gcgtggcacc ggaaatcctg
     1561 cgatcctcct cggcctcgcc ggcacgatgc tggtcgcagc gggtggtctc gccggcggct
     1621 actacgtcgt gtcgacgcgc agtgaagctc gcgccgcaac gcgtccggaa ccgcggtcgt
     1681 ccgacaagca gcagaagccg gtcgctacga acgcgaagcc gacgcctgcg ctcgaagcgc
     1741 tgctgaagaa gaccgtcgat gtgaagaccg gcagcgcgat cgccaagctc acgtgggccg
     1801 acctcggcgt cgagatcgat ccgggcgagc tcggccgttc atcggtcgcg agtgacgcgg
     1861 agctcaacgc gctcgccacc aaaggctcga tcccggtgcg cctcgatcgc gataaggcga
     1921 tcaaagcgct gctccagctc aaaggccaat acgatcgcgg tgcgatcgac gcgtacctcg
     1981 atctcgaaga gcgcgtgatc cacgacgact cgccgggtca aggcatcgac gtgtgggcgt
     2041 ctctccagcg catcgaagca ggtgcgcgcc aagccgcgcc ggccatcgag ctcgtcgcca
     2101 tcggcgtccc cgcgcgcgtc acgaagcaag gcctcggcat cgacgacatc tcgaacgtca
     2161 tgggcgtcta cgagacgaag ttcgcggtcg ccgacaagga tcgcaacttc aacctcaagc
     2221 tcgcggcgtc gcacatcaac ggcgctgtcc tcaagccggg cgaagagtgg tcgttcaacg
     2281 gcaccgtcgg tgagcgctcg caagccaccg gctacaaggt cgcgcacgtc atcaccgcag
     2341 gcgagatggt cgacggcctc gcgggtggca cctgccaaat ctcgacgacg ctgttcggcg
     2401 cgtcgttctt cgcgggcctc gagatcgtga agacgacgaa ccactcgcgg ccgtcggcat
     2461 acacgccgct cggcttcgat gcgacggtcg tgtggccgaa cacggacctc gtgctcaaga
     2521 acccgtacga cttcccggtc gcggtcctct accgcgtcgc gaacggcatc gcccacgtcg
     2581 agatcctcgg caagaagcgg ccgtacacga aggtcgagtt cgctcgtgag gtcctcgagg
     2641 agacgccgta caacaccgaa gaacgcctcg acgaggaaat gccagaaggc ttgacgatgc
     2701 tcgatcagca gggcttcaac ggctacaagc tcaagcgcta tcgccgcttc taccagggca
     2761 agaagatggt gaaggagaac aagtggacgg tgcagtacaa gcccgtcacc gagtacgtgc
     2821 gccgcggcac gaaccccgat ccagcgacgc cggtgccgaa ggagaaggat ctccatccgc
     2881 tgaaggcgcc gaagcccgaa gacttcaaca aagagcgcgt cgtcctcacg cagtagcgca
     2941 ctgggattgg gcgccgcccg agcgcaagtc tcgaggcgcc gagcatggcg gccgacagag
     3001 gctgagcgat cgaggcgggt gagctacgcg ctcacgagcc gtacgggtct tcgacgtctt
     3061 tggggctggc gctcggagcg ctcggcgcct gcgcgacgcc gcagttgaga ttcactttcg
     3121 agcccgacat cgtcacgccg ccgctgccgt tgatcttcac gcccgcatcg gagccccacg
     3181 cggtggcttt gccggagtga atctcgagat tcgagccgcc tttgatctcg gccgacttgg
     3241 cgacgacttt gatctcgccc ttcggcgcct ggaagcagac gtcgccttgc ttggcttcga
     3301 tcgtgatctt gttcgagcca gcgtcgaacg tgatgatgcc gatgccgccg ccgtcctcga
     3361 tgatgagctg gttcttgtcg tcgtcgaacg tgatcttcga gccctgacgc gacttgatga
     3421 tgcggcgcgg gttgccaccc gggttcttgt cggcaggctt gtccttgccg ttccacagcg
     3481 cgccgacgac gagcggccga ttgagatcgc cgtgctcgaa caggacgagc acttcgtcgt
     3541 ccttctcggg gatgaagaac cagccgcggt tcttgctcgc accgagcatg atgatcggcg
     3601 cccaccagct cgtgtcttgg tccgagaggt ttggatactt caccttcacg cggcccagct
     3661 tctgcgggtc cttgttgtcg gtgacgatcg cgatcatcgg ctcgtagacg cgaccgctct
     3721 cgtattcggc ttgcgcgatt tgcgccgcaa acacggacgg aaggtcatag agcaggcgca
     3781 tcggcttgat cttactacgg cttggcgaac cctgggcttc cctgtccgcc agccggccac
     3841 cccctgggta tttcgctaag ggacggacgc aacttccgca accatcaggg acggggctgc
     3901 gttgggccgg gcaatccggg tggttggatg gtcggcgaag gattttgggg cggttgcggt
     3961 tgcggttgca gttgcggttg cggcggcggg ggcggttgcg gttgcggttg cggagtcggg
     4021 gcggtgccgc gaaacggcag gacgccgagc acgatgaggt tcgggtcgtt ggacggggcc
     4081 gcgccggtgg cgagcgcctt ttcgctcttc ggatcgctcg cgacgaggcc gaccgcgagg
     4141 ccatcgcccg tccagcccgg cgggatcgtc acgctgaact tgtcgcggat gtattcgccg
     4201 gcgcgccacc ggtcgctcgc gaagaagcct tcgccgctcg ggcgcatcgt cgacttcgcg
     4261 agatcgctcg gcgcgggatc ggtcggcttc cacgtcgcgg tgttcacggg ccacgcggcg
     4321 agcaggaact tgtaagcgac ctgcgtgcgc gccttcacgt ggaagaagac gttgatgtcg
     4381 acgtgcgcgt tcggcgcggg aagcgagccg ccttggtcga cgctgatgcc gaggacctcg
     4441 agcttggcgt cgtcgagcgt ttggccttgt gtcgtgacct cgggcgttgg cgcggctggc
     4501 agcacgagct ccgccatctt cttgtcggct tggttgaacg tcgcgtcgcg cgcgtacacg
     4561 acacgttcga gccacgcgtc gagggcgctc ttcatgagct gcatcgccgg cggcgacttc
     4621 ggcgcgaggt ttgtcttctc gagcgggtcc gcttgcagat cgtagagctc gtagagattc
     4681 gactgcaggt aatagacgag cttccacgag tcggagatgg cggcgcgaat tggcgagggc
     4741 gcgttcgtct cggcgaacac gatccgatcg tgatcctctt tgccgtagaa gagctgcggc
     4801 acgaggctct tgccctcgaa cgtcaggtcc ttcacgtcga tgcccgtgat ctgcgcgatc
     4861 gtcgggatga cgtcgaggtt cgtcacggcg ccgccgattt gcttgggcgg aaggttcgga
     4921 atgtagaaga cgagcggcac gtgcagcagc tcgcgataga gcgccgtgcc gtgcgtgccg
     4981 acggggacgg aatgctcgcc catgctctcg ccgtgatcgc tcgtgatgat gatgatcgtg
     5041 tggtcgtcgc tcggcaggcg cttgagctcg tcgagcaggc ggccgacttg ctggtccgtc
     5101 cagcgcagct cggcgtcgta gaggtcgggc tcgctcgagc cgtagtcgac gacatcggga
     5161 tgcgcgacat agcggccgtg cggatcgatg tagtgcgccc acagaaacca gcgatgacct
     5221 tgctggcgcg agacccacgc aaggatgtga tcggtcacgc gatccgcagc gacgatgaat
     5281 gggtcggtgc tcttgccgat cgagttgtcg tactcgtcga cgccttgttc gaagccccaa
     5341 tcgttccacc agtgatggga cgagatgacg cccgtgtgat agcccgcccg cttcatgatc
     5401 tcggggagcg tcgtgttctc cggcttgatc ccaggcggag tgcccgcggg cctgttctca
     5461 tcgagcgcga tgcccgagtg gaagaacttc gacgtcatga tcgccggaat cgacgccatc
     5521 gtgccggcgc tcggcgcgtt gcagaacgta aagctcgtcg agcgcttggc gagctctgcg
     5581 agacgcggcg tggtgtcgcg cgccttcgtc gactccttgt agccgccgaa gctcgtgtgg
     5641 tcatagcgga cggtgtcgat cgtgatgaac aggatgttcc agtcgctgcg ccggaactga
     5701 tcggggacgg gcatcgcagg acccgtcgcg atcgcgctcg gcttcagcga gaagtcgcga
     5761 ccgtcgcagt tctgatcgat gttgtcgtcg ggtaagtcct gagcgccggg atggatcgat
     5821 ggcttcagcg ggttgcagtc gttctcgccg agcacggtgc caaagccatc gcgatcgaga
     5881 tcattcgcgg tgcgcacgac cttgatgaga ttcgcgaggg gaggcgaggc ggtcagcgcg
     5941 acatacttcg tctcgacgtc ggcgccccat cgccacagcg acaacacgca gagcgcgagc
     6001 gttgcgccgg caaccgcgag agccgtcgtg cgcttgcgcg ggcgcgcggc gaagcgcggt
     6061 gcgaagtagg cgcccatgcc catgccgagc gcgatcacgc agccagcggt gacgaggcgg
     6121 accggcagca ccgagcgact ctgcggcagc acgtaccaga tcgcgatcag cgccgctgcg
     6181 acgacgccgg tccacgccac gccggctgcg cgccagcgtc cgaacggatt cacgacgccg
     6241 agcaccggcg cgagcgctct tgctgcgacg cgcgttgcga tgctgaacag gcgatgcagc
     6301 ggcacgccga tcgcgacggc gacgagcgca tcgcgcgcga tgtagatgcc gcgcagctgc
     6361 acttcttgat acgcgccggt gagccagacg ctgcgatgct ggatgatgag accgacgaca
     6421 ccgagcgtcg cgaacagtgc ccacgcacgc ggtacgccgt ggcgcacgcc gtgctcgtcg
     6481 agcggcgcgg ggacgaacca cccctcgcgt cgcgcgtcga cgggatcgaa cacggtgcgc
     6541 cacagccgcc acacgagcgt cgcgccgacg agcgccaccg cgagcatgag ccagagccac
     6601 agcgccagcg tcagcgtcag cgcgacgagc ctcagcttgc tcatcgcctc gatgtggccg
     6661 gcatacgcgc gcagggtcag tgcgtactcc accggcgcaa acgcgagcgc accgcccgcc
     6721 gcgacgatga cgatgcggcc gacatccgca cgcatggccg caccgaccga ccggtagcgc
     6781 gggtgatcac ccacggtgcc ccatggcgtc gaacggcagc gttcccggca ggctcaaggc
     6841 tattccggtg gtatcacgtt cgaggtcaac gtgcccgacg cgcgacctcc gcagcgccag
     6901 cgtctacgcc agctcggctg gctcgtcgcg atcgccgtgg cgtacctcta catctttccg
     6961 tacttcccgc gcatccagag cgcgaacgag ctaccgcgcg cctacctcgt gaaggcgatc
     7021 gccgacgacc acacgttcgc gatcgacaag ggcgtcgcga cgtggggcgg taccgcggat
     7081 gtctcgccga gcgacggcca catgtactcg aacaaggcgc cgggcagctc gctcgtcgtc
     7141 gtgccgttct actgggctgt gagtaagatc gcgggaccgc cgagcctcgc gacgaccgtc
     7201 tggatgtgcc gcgtcttcgg cggcgtgatc cctgcgctgc tgtttctgtg gctgctctgg
     7261 ggatttctcg cgcagtacgc gcccgatccg aatgtgcgcc gcctcgtgct gatcgcgtat
     7321 gcgctcggct cgatggcgat gacgtacgcg ctgctctatt actcgcacca gctgagcgcg
     7381 gtctgcatcg gcagcgcgtg gatcctcggg ctcgacgtcg cgaacggacg ccgtcgcgtc
     7441 gcgtggatgg cagcggtcgg gtttctcgcg ggcgcttccg tgctcgtcga ttaccaagcg
     7501 gcgttcgctg gcgtgccgct gctggtctat ctcatcgtgc ggatgtggca gcgcgacgcg
     7561 tcgcagattc tgccagcgct cggcatcgcg gctgcgacgg cagccattcc gatcgcgttg
     7621 ctcctcgcgt atcacgacgt gtgtttcggc ggcccactcc gcacgggcta cgacgcgagc
     7681 acgacgttcg cgcactttca caagcagggt ttcctcggca tcagcaagct gcgaggtgaa
     7741 gcgttctggg gctcgctcgt caaagcggac aacggcctgc tcgtcctctc gccgtggctg
     7801 ctcatcgcga ttccgggcgg catctatctc tggcgacgcg gcgagcgcgg tcacgtcctg
     7861 gccgtcattg cgatcgtcgt catctatgtc gccttcatct cgtcgatcgc gatgtggcgc
     7921 ggcggctggg gcgtcggccc tcgctacatc acggtgatgc tgccgttcct gctgccgctc
     7981 gtcgcgacgg cgctcgaaaa gtggcgcacg caagacctcc tgttcggcgc ggtctccgga
     8041 acgatcgtcg cggcggtcgc gatctactcg ctgtcgagcg tcacgtttcc gtattggcct
     8101 gactcgctga agaacccgct gtacgaagtg acgttccgtc tgctccacga caacgccgtc
     8161 gctccaaacc ttggcagtgc gctcggcatc cgcggcgtcg ccggcatcgt gccgtacctc
     8221 ctctgcgtgt tcgggctact cgcatacacg ctctaccgcg ccgggaacgt gcgtgtgctc
     8281 gcagttgctg cggcggtcgg cctcgcgatc ctcgtcgcgt tccggttcgc gccgctcact
     8341 ggcccgcacg ccgacgtcgc ctacaagaac acggtgtttc cagcggtgac gaagtgatgc
     8401 tacgacatgc ctccatgcgc gcttgggtgc cgctgcttct gctcgccgct ctcatcggtg
     8461 gctgtaaaga caagaagcgc gcgtcgacgt acgaggatgc cggcacgggc tcgaacgcga
     8521 tcgatccgag tgagctcgcc gcacgctacg acaagaagtg cgtcgccggc gatctcgaag
     8581 cgtgtcgcgc actcggcgtg atgtacacgg aaggcacggg cgtctcgccg gatccgcgcc
     8641 gtgccgccgc gctgttcggc caagcctgcg cgggagacaa cctctccgca tgcaaccacc
     8701 tcgcgctcgc actctccgaa ggcatgggcg tcacgaaaga cgtcgcacgc gccgtcgacg
     8761 tgtaccagaa ggcgtgcgac cgcggcttcg tgctctcgtg ccgcaacctc ggcctcatgc
     8821 tgcgcgacgg ccgcggcgtc gacgtcgacc tcgctcgcgc ggacaagctg ctgagcaccg
     8881 catgcaaagg caagatccca ttcgcgtgca cgaacgccgg cgacctcgac tcgctgcttg
     8941 cagcgaagca gcctgcaggt agcaccgcgc agaagcagca tgcggccgca gcgatcgcgc
     9001 actaccgcct cggctgtgac gcgggcgacc cgactgcgtg tcgccagatc ggaatcgcgt
     9061 acctcgaagc gcgcggcctg ccacgttcga cggctgcagc cgcggtgtgg ctcgagcgtg
     9121 cgtgcgtcgg tgacgatgcg atcgcgtgtc gcgtgctcgg cgcgatgaac atggaaggcg
     9181 tcggagtcgc aaaagacaca aagcgtggcc aagagcttct gcagcgcgcg tgcgatcgca
     9241 aagacgatga agcgtgtcgg ctcgtgaagc tcggcgctgc agcgggcagc aacgcagcag
     9301 ctgcgggcag caacgctgcc gccgtcggca gtggctccgc ggcttctcaa tagtcgctcg
     9361 cgcttctcgg tagtcgctcg cagcttctcg gtagtcgctc gcggcgtgtc gtagcggctc
     9421 gcggcttctc ggtagtcgct cgcagcttct cggtagtcgc tcgcagcgtg tcgtagcggc
     9481 tcgcggcttc tcgatagtgg ctcgaggcgc ccgactcgac tggcgcttcc acaggcagcc
     9541 gctgaacgcg tggagccgtc acgtggtgga ggtgcgagct cgtcgacgcc tgattaaccc
     9601 agctccggat gcgctgaatg gggtggcgga gtggcgccga aatcggctca tgatccgggt
     9661 cgaggtcgcc tcactgaccg tcctttcgcg gcgcgctccg gaccgggagt acgtggccta
     9721 taccaccatg taggtatacg gccgactccg ccggacacac gagctaagtt gttgaggcgc
     9781 cgtcgctccc ggaaagtgcc gacacaccg
//
LOCUS       DATLBZ010000020         8648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6716660, whole genome shotgun sequence.
ACCESSION   DATLBZ010000020 DATLBZ010000000
VERSION     DATLBZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6716660"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1860
                     /locus_tag="VMZ53_00845"
     CDS             <1..1860
                     /locus_tag="VMZ53_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27015.1"
                     /translation="GRTGYLRMVSLADITQTKGITLAAAFDSGFPKLGVATNPTVKTS
                     PNQLGALNIAAGRLYVTSVSASPEGPPRFDNNVYPVVYVADLASAAEVRDASGTVNLA
                     RKIYDAIPSPSATNPRFLPGDLTDIDFVPGSTVGYAIGRAGDVMVRVTFAADVQIGST
                     QNKLIDLAGNDTIGKCQGPTGLIVDKNHMRAYVNCWMTRKLGVVDLASQSLTSAIEAA
                     PTPTSAAELSVHKGRRFYFTGRGRWSANVANGAKGGEGWSSCGSCHPDGLTDNMTWIF
                     GAGPRQTTSQDGSFSHGPGAQKQRIFNWTGIFDEHHDFERNTRDTSGGLGAITTAPSL
                     AECDQLDKETRVNIDAFGGLGKPLKEVMEDSSLALCAHKDWDDIDNFVKSVQPVKAKR
                     TTDTAAIARGRQLFTDGNCAKCHGGSGWTVSRRFFLPSGATNAALATAPFTIPSFFPT
                     TFMYNADQQSTPTKRMASSTQPPIPSDLTGPGEAAEVKVAQVACSLRNVGTFGVPGDT
                     AATDALELRPGALGRAEGRAGFNVPSLYGLSIGAPYLHHGQSPSLTDLFTNSKWQFHT
                     NAGNANFSLSLTSSSVADLVAFLTSIDAGATEFAVPSDANTGGSFDACPLQFP"
     gene            complement(1938..3056)
                     /locus_tag="VMZ53_00850"
     CDS             complement(1938..3056)
                     /locus_tag="VMZ53_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HUS27016.1"
                     /translation="MRLLAVLALVGACSFEHGTLGSAADGGVDTTDTGPDRWVYRKPV
                     TILGSHVRNGAHIRFVALIAWDVDDALAKHARADGHDIRFVTTDGVKMPYERVAYNGA
                     TGGLQAWVQVPSIQQSQDTPFFIEYGNPNASDQQDAAATWPPNVVGAWHLESGDDSTG
                     NNNDAQAVNGAMIGVKAKVGLGASLDGIDDRLLMPNSASLDAVATQGTISLWLKLTDA
                     TAVTRQFIMASSNDITPPKSSFEWAVSTSGDTYFYPRRGDGDVAFNSITNPFTDGRWH
                     HAAVRLQFSSKAVEFFIDGMQTTLTTNNAATMWMMLADPDDWLWGSHPLYAAPLLGAM
                     DEIRVVTALRGSGWLATEYLNQNDPPGFIRVDPEELVP"
     gene            3086..3379
                     /locus_tag="VMZ53_00855"
     CDS             3086..3379
                     /locus_tag="VMZ53_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HUS27017.1"
                     /translation="MRRLVLALALLTPVMFGCDDERPRSAPTEIQGPLDLNRATTKQL
                     EQLPGIGDHLARSIIASRNARGGHFNSVDDLLKIDGIGDKTLEKIRPLVYVGP"
     gene            complement(3386..5998)
                     /locus_tag="VMZ53_00860"
     CDS             complement(3386..5998)
                     /locus_tag="VMZ53_00860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27018.1"
                     /translation="MLLGFTARETYLRGQYPDVGMEVDSALGVEARDGRWVGVLQRDH
                     VVALDGEPVRSPADWTARALAHHGPMTVTFKRGDKTFEVTASPTPLEWMDEGAMWARL
                     ATATALMLMALIVFVLRPGVAMTWLLFAFAWDLGAFLLVRIAFWSDPHLLKIVQIYPW
                     AAASALGLHLLCHYPQRLSWLTRTRAVLMYLPLPAGAVMHAMGAEVKGSLLGTLYAVI
                     ITGVVVTVMVTQHRAVKRSNDARAQSQYRALIVGFAVGLAVPAVWNWLRITADIWNSP
                     WAAHYNALPLVVFIGVISYASVRHNALAIDRFTAAVVGYTMTTILLGGAFAAALVGIP
                     LLVGTDSPALLVGTTALTFASFSPAYRLVKGWVDRRFFREQAGAQVIADALRDLVLDM
                     QQATRDDAMTAAFKAAAILRGDRVEVWMLHTETKQLRAERREGSGQGPAVPLPLDGPL
                     GKALAAGVSAGIGELAPRSFESAAQEELWLRELAMIAPVMVRGVVAGFVGVGRKRSGA
                     GYTIEELSFLTIIGAQLGAVVERTESQSQIDRYHLERRLGTGGMAEVFLAWQVGPGGF
                     ERKVALKRPLPHVAEDPNAVASFLDEARLAAQLVHPHIAQVYDVGEKSGTYFIVMEYV
                     DGPSIRELLRDMHAQSRVMPVSVATTIMVDVLSALDYAHSARDERGRKLELVHRDVSP
                     RNILMNRAGQAKLVDFGIARAQFQLHITRTGIVKGTLPYMPPEQANDMPLDHRADLYS
                     AGVVLYELLTVTPAFPKGPVGRRPKPASSIVADVPPTLDVVLRKSTEQSPTDRYASAK
                     EMADAILEAIKPLRPAEPADVVKFLRSISPKAMRGHDVRVSQPIDVPPLPPDDEEEAG
                     DATVQMGKRPTDVD"
     gene            complement(6119..7414)
                     /locus_tag="VMZ53_00865"
     CDS             complement(6119..7414)
                     /locus_tag="VMZ53_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27019.1"
                     /translation="MRALAVVVLVAACGRFGFAPDETVDANADADVDALVGTAHVDIH
                     VAGVGAVNIGGTWCAGTCGVDLPLGPVALQIVPGDGQEVVADTSVCPLGCDVLRGDSV
                     IDVTFAPAITANVVFITSIGPALPFGGLSGADAFCQQRAVAGGLSGTFIAMLSTTTVN
                     LRDRLTGRGWVRRDGRTVFDLNSDVFLRQVNPIALDEFGNAKMGGIFPSATNDDGTVA
                     PGTTCGDWTAMTGLTEVRQPGFSGAKYETGNNGPCDQLQALFCAEVGKNVPTVAAKWP
                     FGRYAFLTSVAFDPALGIAAADALCGSQASAASLPGTYRALLADVGTTAASRFTSLAG
                     PWRRTDGALITLGDISGPIDTPLNRAANGGPAGIARAWLGGYDANTVGTAASTCNGWT
                     TNASGATGAFHFAYDTRAWAQNGNFGCPQGPVGLFCLQE"
     gene            complement(7487..8119)
                     /locus_tag="VMZ53_00870"
     CDS             complement(7487..8119)
                     /locus_tag="VMZ53_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27020.1"
                     /translation="MKPLKSLLVLAIMLAPGIAAAQGYYGRGGGYQSAPAAQIPGGFH
                     NRMGRLAYGFSLGLGGMHDRGSNITSCMNCDYNPIVVEVDGHIGGFLTPRFALMGEVQ
                     TNGRTVHSDFYNGDTVLFSNALMIAGQYWLTPQLWVKGGIGFAHLDAQDDYWQYDLGG
                     GTAIMGGVGYEVMSARNFSVDLQGRLIQANYHGVNDSITSATVGVGINWY"
     gene            complement(8232..>8648)
                     /locus_tag="VMZ53_00875"
     CDS             complement(8232..>8648)
                     /locus_tag="VMZ53_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27021.1"
                     /translation="EREAKLCGIAAGFGLAVIEMSEADRMRWLGFLARIERRAEKRVL
                     IGAEPSRLAELQAGLAGCGYAVVGGTDPGALVQLASGDTRPADAVLIDAGWLQNDASA
                     SLVESLFSARNVPCVTMNGEVRRARQAIDRLLEVVV"
BASE COUNT         1508 a         2919 c         2794 g         1427 t
ORIGIN      
        1 ggccgcactg gctacctgcg catggtgtcg ctcgccgaca tcacgcagac gaagggcatc
       61 acgctcgccg ccgcgttcga cagcggcttc cccaagctcg gcgtcgcgac gaacccgacg
      121 gtcaagacat ctccgaacca gctcggcgcg ctgaacatcg cagccggccg cctctacgtg
      181 acgagcgtgt ccgcttcccc cgagggcccg ccacgcttcg acaacaacgt ctatcccgtc
      241 gtctacgtcg ccgacctcgc gagcgctgca gaagtgcgtg acgcgagcgg caccgtgaac
      301 ctcgcgcgca agatctacga cgcgattccg agcccgtcgg cgacgaaccc gcgcttcttg
      361 cccggcgatt tgacggacat cgatttcgtc cccggctcga cggtcggcta cgcgatcggc
      421 cgcgcgggcg acgtgatggt gcgcgtgacg tttgccgccg acgtccagat tggctcgacg
      481 cagaacaagc tgatcgacct cgcgggcaac gacacgatcg gcaagtgcca aggcccgacc
      541 ggcctaatcg tcgacaagaa tcacatgcgc gcgtacgtga actgttggat gacgcgcaag
      601 ctcggcgtcg tcgacctcgc gtcgcaatcg ctgaccagcg cgatcgaagc tgcgccgact
      661 ccgaccagcg cggcggagct cagcgtgcac aaaggccgtc gcttctactt caccggccgc
      721 ggccggtgga gcgcgaacgt ggcaaacggt gcgaagggtg gcgaaggctg gtcgtcgtgc
      781 ggctcgtgcc atccggacgg gctgacggac aacatgacct ggatcttcgg cgcgggtcct
      841 cgccagacga cgtcgcaaga cggctcgttc tcgcacggtc ctggtgcgca gaagcagcgc
      901 atcttcaact ggaccggcat tttcgacgag catcacgact tcgagcgcaa cacgcgcgac
      961 acgtcgggtg ggctcggcgc gatcacgacg gctccgtcgc ttgccgagtg cgatcagctc
     1021 gacaaagaga cgcgcgtcaa catcgatgcg ttcggtggtc tcggcaagcc gctcaaggaa
     1081 gtcatggagg attcgtcgct cgcgctctgc gctcacaaag actgggatga catcgacaac
     1141 ttcgtgaaga gcgtgcaacc cgtgaaggcg aagcgcacga cggacaccgc tgcaatcgcg
     1201 cgaggtcgcc agctgttcac cgacggcaat tgcgcgaagt gccacggtgg aagtggctgg
     1261 acggtgtcgc gccgcttctt cctgccgtcg ggtgcgacga atgccgcgct cgccacggcg
     1321 ccgttcacga tcccgagctt cttcccgacg acgttcatgt acaacgccga tcagcagtcg
     1381 acgcccacga agcgcatggc atcgtcgacg cagccgccga ttccgtccga tctgacgggc
     1441 cccggcgaag ccgccgaggt gaaggtcgcg caggtcgcgt gctcgctgcg caacgtcggt
     1501 acgttcggcg ttcccggtga caccgccgca acggatgcgc tcgagcttcg cccgggtgcg
     1561 ctcggtcgcg ccgaaggtcg cgctggcttc aacgtgccgt cgctctacgg cctctcgatc
     1621 ggcgcgccgt atctgcatca cggtcaatcg ccgtcgctca cggacctgtt cacgaacagc
     1681 aagtggcagt tccacacgaa cgccggcaac gcgaacttca gcttgtcgct cacttcgtcg
     1741 tcggtcgcgg acctcgtcgc gttcctgacg tcgatcgacg cgggtgcaac ggaattcgcg
     1801 gttccgtcgg acgcgaatac cggcgggagc ttcgacgcat gcccgctgca gttcccctag
     1861 tttcccgagc cgtgcactga ggtcagtcag ctcgggggcc cgcgtgtcga atgttcggcg
     1921 cgcgggcttt atcttttcta aggcacgagc tcctcgggat cgacgcgaat gaagccgggt
     1981 gggtcgttct gattcaagta ttcggtcgcg agccagcccg agccgcgcag cgcagtgacg
     2041 acgcgaatct cgtccatcgc accgaggagc ggcgcggcat agagcggatg gctgccccac
     2101 agccagtcat ccgggtcggc gagcatcatc cacatcgtcg ctgcgttgtt cgtcgtcagc
     2161 gtcgtctgca tgccgtcgat gaagaactcg accgccttcg agctgaactg caggcgcaca
     2221 gcagcgtgat gccagcgccc gtcggtgaat ggattcgtga tcgagttgaa ggcgacgtcg
     2281 ccgtcgccgc ggcgcgggta gaaataggtg tcaccactcg tcgacacggc ccactcgaac
     2341 gacgacttcg gcggcgtgat gtcgttgctc gacgccatga tgaactgccg cgtcactgcc
     2401 gtcgcatcgg tcagcttgag ccacaggctg atcgttccct gcgttgcgac ggcatcgagt
     2461 gatgcactgt tcggcatcag caagcgatcg tcgatcccat cgaggctcgc gccgagcccg
     2521 accttggcct tcacgccgat catcgcgccg ttcacggcct gcgcgtcgtt gttgttcccg
     2581 gtcgagtcgt cgccgctctc gaggtgccac gcaccaacga cattgggagg ccaggtcgcg
     2641 gccgcgtctt gttgatcact cgcgttcggg ttgccgtact cgatgaagaa cggcgtgtcc
     2701 tggctctgtt ggatcgacgg cacctgcacc catgcctgca gaccgccggt cgcgccgtta
     2761 tacgccacgc gctcgtacgg cattttcacg ccgtccgtcg tgacgaagcg aatgtcatgg
     2821 ccatcggcgc gcgcgtgctt cgcgagtgca tcgtcgacgt cccacgcgat cagcgcgacg
     2881 aaccggatgt gcgcaccgtt gcgcacgtgt gagccaagga tcgtgaccgg cttgcggtag
     2941 acccagcggt ccggccccgt gtctgtcgtg tccacgccgc cgtcagccgc gctcccgagc
     3001 gtcccgtgct cgaagctaca ggctcccacg agcgcgagca ccgccagcaa tcgcacgagc
     3061 cgatcatatc cgatacgctg gaggcatgcg tcgccttgtc ctcgcgctcg cgctgctcac
     3121 acccgtcatg ttcggttgcg acgacgaacg gcctcgctcc gcaccgacgg agatccaagg
     3181 gccgctcgac ctcaatcgcg cgacgacgaa acagctcgag cagctcccgg gcatcggcga
     3241 tcacctcgcg cgcagcatca tcgcgtcgcg caacgcacga ggcgggcact tcaactccgt
     3301 cgacgatctg ctgaagatcg atggcatcgg cgacaagacg ctcgagaaga tccgaccgct
     3361 cgtgtacgtc gggccctagt cggccctagt cgacatcggt cgggcgcttg cccatctgca
     3421 cggtggcgtc gccggcttcc tcttcgtcgt cgggtggaag cggcggcaca tcgatcggct
     3481 gcgacacgcg cacgtcgtgg ccgcgcatcg ccttcggcga gatcgagcgc aagaacttca
     3541 cgacgtcggc aggctcggct gggcgcagcg gcttgatggc ttcgaggatc gcatcagcca
     3601 tctccttcgc cgacgcatag cgatcggtcg gggattgctc ggtcgacttg cgcagcacga
     3661 cgtcgagcgt aggcgggaca tcggctacga tcgacgatgc gggtttcggc cgccgcccga
     3721 ccggtccctt cggaaacgcc ggcgtcacgg tgagcagctc gtagagcacg acgccggcgg
     3781 agtagaggtc ggcgcggtga tcgagcggca tgtcgttcgc ttgctcggga ggcatgtacg
     3841 gtagcgtgcc tttgacgatg cccgtgcgcg tgatgtggag ctggaactgc gcgcgcgcga
     3901 tgccgaagtc gacgagcttt gcttggccgg cgcgattcat gaggatgttg cgcggcgaaa
     3961 cgtcgcgatg cacgagctcg agcttgcgac cgcgttcgtc gcgcgcgctg tgtgcgtagt
     4021 cgagcgccga caggacgtcg accatgattg tcgttgcgac cgagacgggc atcacgcgcg
     4081 attgcgcatg catgtcgcga agcagctcgc gaatcgatgg cccatcgacg tactccataa
     4141 cgatgaagta cgtgccgctc ttctcgccga cgtcgtagac ctgcgcgatg tgcggatgca
     4201 cgagctgcgc ggcgagtcgc gcctcgtcga gaaacgacgc gacggcgttc ggatcttccg
     4261 cgacgtgggg cagcggccgc ttgagtgcga ccttgcgctc gaagccgccg gggccgacct
     4321 gccaggcgag gaagacctcg gccatgccgc ccgtgccgag ccgccgctcg aggtgatagc
     4381 gatcgatctg cgactgactc tccgtgcgct cgacgacggc gccgagctgc gcgccgatga
     4441 tcgtcagaaa cgacagctct tcgatcgtgt agccggcgcc cgaacgcttg cgaccgacgc
     4501 cgacgaagcc agcgacgacg ccgcgcacca tcaccggcgc gatcatcgcg agctctcgca
     4561 gccacagctc ttcttgcgcc gctgattcga acgagcgcgg agcaagctcg ccgatgcctg
     4621 cgctcacccc tgccgcgagc gccttgccga gcgggccgtc gagcggaagc ggaaccgcgg
     4681 gtccctgtcc ggatccctcc cgacgctcgg cgcgcaactg cttcgtctcg gtgtgcagca
     4741 tccagacttc gacgcggtcg cctcggagaa tcgcggcggc tttgaatgcg gccgtcatcg
     4801 cgtcgtcgcg cgtggcctgc tgcatgtcga gcacgagatc gcgcaatgca tctgcgatca
     4861 cctgcgcacc tgcttgctcg cggaagaagc ggcgatcgac ccagcctttc accaggcgat
     4921 atgccggcga gaagctcgcg aacgtcagcg cggtcgtgcc gacgagcaac gcaggtgagt
     4981 ccgtaccgac gagcagcgga atgccgacga gcgcagcggc gaatgcgccg cccagcaaga
     5041 tcgtcgtcat cgtgtaaccg acgaccgcgg cggtgaagcg atcgatcgcg agcgcgttgt
     5101 gacgaacact ggcgtacgaa ataacgccga tgaagacgac gagcggcagc gcgttgtagt
     5161 gcgcggccca cggcgagttc cagatgtccg cggtgatgcg cagccaattc cagaccgcgg
     5221 gcaccgcgag cccgacggcg aagccgacga tcagcgcgcg atattgcgac tgcgcgcgtg
     5281 cgtcattgct gcgcttcacc gcgcgatgtt gcgtgaccat caccgtgacg acgacgccgg
     5341 tgatgatcac ggcgtagagc gtgccgagca gcgagccctt cacctccgcg cccatcgcgt
     5401 gcatcactgc gcctgctggc agcggcaagt acatcagcac cgcgcgcgtg cgggtgagcc
     5461 acgagagccg ctgcgggtaa tgacacagca agtgaagacc gagcgcgctc gccgcggccc
     5521 acggatagat ctggacgatc ttgagcagat gcggatcgga ccagaacgcg atgcgcacga
     5581 ggagaaacgc gccgaggtcc cacgcgaacg cgaacaagag ccacgtcatc gcgacgcccg
     5641 gccgcagcac gaacacgatc agcgccatga gcatcagcgc ggtcgccgtc gcgagcctcg
     5701 cccacatcgc cccctcgtcc atccactcga gcggcgtggg tgatgccgtc acctcgaacg
     5761 tcttgtcgcc gcgcttgaac gtgaccgtca tcggaccgtg atgcgcgagc gcgcgtgcag
     5821 tccagtccgc cggactgcgc acgggctctc cgtcgagcgc cacgacgtga tcgcgttgga
     5881 ggacgcctac ccacctgcca tcgcgcgcct cgacgccgag cgccgagtcg acctccatgc
     5941 cgacgtctgg atactgaccg cgaagatacg tctcgcgtgc ggtgaacccg agcagcacgg
     6001 gcgtgatcaa cgcgatgaag atgacgatcg aaagaccgcg cgtcgcagac agccgactga
     6061 gcagtgtgat cgcacctgcc gtttgggtct gagtggtcag gatcccagtg tacgccgtct
     6121 attcctgcag gcagaacaac ccgacggggc cctgcgggca gccgaagttc ccgttttgcg
     6181 cccacgcgcg cgtgtcatac gcaaagtgaa aggcgccggt cgcgccgctc gcgttcgttg
     6241 tccaaccgtt gcaggtcgat gccgccgtac caacggtgtt cgcgtcataa ccgccgagcc
     6301 aagcgcgcgc gattccggcg ggcccaccat tggcggcgcg attgagcggc gtgtcgatcg
     6361 gccccgagat atcgccgagc gtgatgagcg ctccgtcggt tcgccgccag ggtcctgcga
     6421 gcgacgtgaa gcgactcgct gcggtcgtgc cgacatcggc gagaagcgcg cgatacgtgc
     6481 cgggcaagct cgccgcactt gcttgcgaac cacacagcgc atcggcggcc gcgatgccca
     6541 gcgctggatc gaacgcgacc gacgtcagga atgcgtagcg accgaacggc cacttcgccg
     6601 caacggtcgg aacgttcttg ccgacctccg cgcagaacag cgcctgcagc tgatcgcacg
     6661 gaccgttgtt cccggtttcg tacttcgcgc ccgagaaccc gggttgccgc acctcggtga
     6721 gccccgtcat cgcggtccaa tcaccgcatg tcgttccggg cgccaccgtg ccgtcatcgt
     6781 tcgtcgcgct cggaaagatg ccgcccattt tcgcgtttcc gaattcgtcg agtgcgatcg
     6841 gattcacttg gcgcaggaag acgtcgctat tgagatcgaa caccgtgcgt ccgtcgcggc
     6901 gcacccaccc tcggcccgtt agtcgatcgc gcaggttcac cgtcgtggtc gacagcatcg
     6961 cgatgaacgt tccggaaagc ccaccagcga cggcgcgctg ctggcagaac gcgtctgcac
     7021 cgctgagccc gccaaaggga agtgcgggtc cgatcgacgt gatgaacacg acgttggccg
     7081 tgatcgccgg cgcgaacgtg acgtcgatga cggagtcgcc acgcagcacg tcacagccaa
     7141 gaggacacac gctcgtgtcc gcgacgacct cttgtccatc gccgggaacg atctggagcg
     7201 cgaccggtcc cagcgggaga tcgacgccgc acgtacccgc gcaccacgtt ccgccgatgt
     7261 tgaccgcgcc cacccctgcg acgtgaatgt cgacgtgcgc agtcccgacg agcgcatcga
     7321 cgtccgcatc cgcattcgcg tcgacggtct catcgggcgc aaagccaaat cggccacacg
     7381 ctgcgaccag cacgacaact gcgagcgcgc gcatgcctca tcatctcata gcccgaaaac
     7441 gcgaacgccc ccgagttggg ggcgcttgcg gcgagaagct ctctcgctag taccagttga
     7501 taccgacgcc aaccgtcgcc gacgtgatgc tgtcgttcac gccgtggtag ttcgcctgaa
     7561 tgaggcggcc ctggagatcg acggagaagt tacgcgccga catcacctcg tagccgacgc
     7621 cgcccatgat ggccgtgccg ccgccgaggt cgtactgcca atagtcgtcc tgcgcgtcga
     7681 gatgcgcgaa gccgatgccg cccttcaccc agagctgcgg ggtgagccag tactggccgg
     7741 cgatcatgag cgcgttgctg aacagcaccg tgtcgccgtt gtagaagtcg gagtgaacgg
     7801 tgcggccgtt cgtctggact tcgcccatca gcgcgaagcg cggcgtgaga aatccgccga
     7861 tgtggccgtc gacttcgacg acgatcgggt tgtagtcgca gttcatgcag ctcgtgatgt
     7921 tgctgccgcg gtcgtgcatg ccgccgagcc cgaggctgaa gccgtacgcg agacggccca
     7981 tgcggttgtg gaagccgccc ggaatctgtg cggccggcgc ggactgatag ccgccaccgc
     8041 ggccgtaata gccttgggca gccgcaatgc ccggagcgag catgatggcg agcacgagga
     8101 gtgacttgag tggcttcatg gcccctctat tgtcgcaagt ccggggccag tacgcgaccg
     8161 gtcaaagcca gcctgtttcc acggcttagc tggtcggacc ggtcaacgcc cgttcacagc
     8221 gaggcgtggt ttcacacgac cacttcgagc aggcgatcaa tggcttggcg cgcgcggcga
     8281 acctcgccgt tcatcgtcac gcacggcacg ttgcgcgcgg agaacaggct ctcgacgagc
     8341 gaggccgagg cgtcgttctg cagccagccc gcgtcgatga gcacggcgtc ggccggccgc
     8401 gtgtcgcccg atgcaagctg cacgagggcg cccggatccg tgccccccac aaccgcgtag
     8461 ccgcaaccgg cgagacccgc ctgcagctcg gcgaggcgtg acggctcagc gccgatgaga
     8521 acgcgcttct ccgcacggcg ttcgattcgc gcaaggaagc cgagccaccg catgcgatcc
     8581 gcttcgctca tctcgatcac cgcgagcccg aatccggcgg cgatcccaca cagctttgct
     8641 tcgcgctc
//
LOCUS       DATLBZ010000021         8238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1509902, whole genome shotgun sequence.
ACCESSION   DATLBZ010000021 DATLBZ010000000
VERSION     DATLBZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509902"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VMZ53_00880"
     CDS             complement(<1..792)
                     /locus_tag="VMZ53_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HUS27022.1"
                     /translation="MVVRSATKTDPKLNVEVFRQANSRTNTLFSPVAIASDGERLFVN
                     SRFMFYRSDDNGRLENVLTGEHTNSWTSGLIVTSEALYAFGQDGIIRSADAGASFKPV
                     LEPSSVRAMAAEPNGTLWAGDEDGVILVKLRGAKSFTAIDQRTAGVTAMCQSTLGVFI
                     GDEEGRLWIGRTGKLSATSLQTGEPIRAVLETDRGTILAISGGEHNQKPTLARSTNGG
                     KSFKTTPFKGSLLVLAQIPDGGPIIAGGWEGLLAVSRDDGSTFERV"
     gene            907..2949
                     /locus_tag="VMZ53_00885"
     CDS             907..2949
                     /locus_tag="VMZ53_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HUS27023.1"
                     /translation="MRGETADGSDASISGSLAVGTLHGPWRIEYELGRGAMGIVYAVT
                     HEHIGKRAALKVLHRTLGVGTTKYAERMLLEARVVNAIAHPNIVDIFDVGATEDGRPY
                     IVMERVDGCALGDAALDRADALEVLGQIADALGAAHAVGVVHRDIKPDNILLVCSRDG
                     AVCVKLVDWGIARMLNSDVRHTFEGQIVGTPLYLSPEQACGAPVTAQTDVYSLGVVAY
                     ELLLGEPPFRGATREVMAKHLWVEPRDPRTLWPEIPDALASLLLAMLAKAPEARPVMT
                     SVAATIAAARIRRGDETPAEALEPSSSESELREAVARRALRPNQMFAIVAGAALIGAI
                     ALIVAVTRASSEGRAVPKVAVPSVAERAPAEEPVAMPTAPQARRSPVVTSTRATTAPA
                     QPSGVVENCPDPSLLRDGAEWVMVCTGRHGDDIYPMHVSSDLRSWRDAGFIFTAATRP
                     TWADGNYWSPEVRRTSDGYAAYFSMRVSGARNAIGVATSQSARGPFVDRGEPLYAPRD
                     GASDPDVFLDGGRTFLFQRRNTPHATLWVQELAADATPVGDARQVLAPSEEWEQGNVQ
                     VPAVIRRGDYVYLFYSGATYCDRSYAIGVARSRSPLGPFEKMRTPLVQSNDEWLGPGH
                     ASVAMTGTGEYVIAYHGYRASEGEPSCVRGANGDNNARHARVERLTFENGWPRLRD"
     gene            complement(2946..3656)
                     /locus_tag="VMZ53_00890"
     CDS             complement(2946..3656)
                     /locus_tag="VMZ53_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HUS27024.1"
                     /translation="MPPKLLHGKTVVFTGKLTMTRAEAKAMAERSGASVVSTVTNACN
                     VIVTGSRTGSKLDKALAKGIEIWDEADFIRRMTVEEHQGRLSVNKGRIIKKTGKTQFQ
                     FSLAWDAHVDLDLHCHTPKGDVYFGNKKEAGVTLDVDRMPGVDRWNHRKKTWDVDPVE
                     NIVCNRAKPGNYKVTVVLYSNHYHGRGAAPFTVHYRLGDYEDVFRGKLTRRGSEAKIC
                     EFRVAPNGTFKIRHTKKN"
     gene            complement(3696..4601)
                     /locus_tag="VMZ53_00895"
     CDS             complement(3696..4601)
                     /locus_tag="VMZ53_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HUS27025.1"
                     /translation="MSKKHPPISVWDRKHGREIEEKVYGEGSVRFMYANPFGRGLGDA
                     LLSRRWFSQSYGWLQSTRWSGRKVEPFIARYGIPMEEYEPGPFPTFNDFFIRKFREGA
                     RPCVQDPHVMAAFAEARYLAWTSIEDDQTFPVKGEHLSAGSLMGSDDHARDFIGGPLL
                     LARLCPVDYHRFHYPDDGRVLDSYRAQGAFHSVNPVALKAKSNVLATNERHISILETA
                     NFGKLAYIEVGAMMVGKIVQSHVEPSFKRGDEKGYFLFGGSTVVVLGEPGRWRPDAEI
                     LQQTAQQRETLIRLGEPVATRTLSP"
     gene            4660..7284
                     /locus_tag="VMZ53_00900"
     CDS             4660..7284
                     /locus_tag="VMZ53_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUS27026.1"
                     /translation="MTIQAPSFELLLVGDLLPANAAFVTLSHDADSVTVMSGDRKIRA
                     SPLYAGRVTDGNGARSYAIVHPSDGRWPTGTLRVVARNRQGVVAECTVTVGPPIHGSQ
                     PTCTYVSGPTEVGTRAGFLYGGLADSHAPVVAIIVDVKSGDRSRTRVFIGDAVRGCGG
                     VALGAFDEVTRVRITNVLGNYIELFPPFPHGVAPAGPFECVSDDLNHLLCNAAYENDF
                     EAVRAALADGADVRARASRGGFAAWTPLAWAATPAMAELLLAAGAEIDAEDADGYTPM
                     FRALLYKRLDVADFLLQRGADPNYCASPEHTLPVDQALQVSPEVGLRVLRAGGRVGPK
                     TYASLWLKQDSRALLDKWPILGAPIADRDKELMLAARSGPLDAVTTLLRDGASVAARD
                     TAGASALDHALLKGNIEAARLLQRAELATLDNAKLFLMTLDDYDTSLRERASSFDLEA
                     RTPRGMTALMLAARYRRHRALGALLDLGANIEQRNGTSTPLVAAIESNNLYGMLMLLD
                     RGAVMNLQIDGANPVLTAAIKADSDLLKHLLARGLEVPPGQYTKYDETGRLLTNVLAG
                     IRPYVGPPTFSSWSDCKICRELPERMYWCRSAMGEVTGDGIPPITEQQFETFDRYLWK
                     CPYCATYYDFEHDHDNGLTDGWDRDDLTRISLDIARQRLDAIPKRSSEFPAMAEGRIE
                     REIARAAFASALATPGKKVQISLDEVKDGRRPLLIYELAPGAKRIAWHRVPDSQHDVL
                     VAELKARGLEPAETDSDSMNDFIWIRTATGLEVYDAHHKVLDVAEDCATLRDGSVIVR
                     GDVARVIAYASRNYVERGVKVVLRSGVEIDLVADYSISAEIYYQYNRNDLLFETLWCA
                     RIGHAVADWAGMKLENLI"
     gene            complement(7289..>8238)
                     /locus_tag="VMZ53_00905"
     CDS             complement(7289..>8238)
                     /locus_tag="VMZ53_00905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27027.1"
                     /translation="TENTALNAALLATGMPPPADSLALGATLTSLAPKGLTPSTRVAP
                     PTLQPGQLFDGRYRIEARIGRGGPATVYRAQHVALHEPRAIKLLHPESLETPGAVEQL
                     RREVDATSRIHHPNVVQLHDFGRSVEGHYYLSLELVDGESLAATLHRDGPLPVARALA
                     YARGILGALQAAHQLHLIHRDLKPGNVLIDRRDQPRVTDFGMAQSLHDYRSAHDTIAG
                     TPAYMSPEQCDGKPLDGRTDLYSFGVTLYHMLSGALPYRDTSGDPFKLALSTDRVPLE
                     QRVPALAPELVTTVARCLAIDPAARPPSALAVLRELETL"
BASE COUNT         1353 a         2562 c         2826 g         1497 t
ORIGIN      
        1 gacgcgctcg aacgtgctgc cgtcgtctcg cgatactgcc aggagcccct cccagccgcc
       61 ggcgatgatc gggccaccgt cggggatctg agccaggacg agcagcgatc ctttgaaagg
      121 cgtcgtcttg aaggatttcc ctccgttcgt cgagcgagcc aacgtcggct tctgattgtg
      181 ttcgccgccg ctgatcgcca agatcgtgcc tcgatcggtc tcgaggacgg cacgaatggg
      241 ctcgcctgtt tggagcgacg ttgccgagag cttgcctgtt ctcccgatcc acagtcggcc
      301 ctcctcatcg ccgatgaaca cgcccaacgt gctctggcac atcgctgtaa cgccggccgt
      361 gcgctgatcg atcgcggtga acgacttcgc gccgcgcagc ttcacgagga tgacgccgtc
      421 ttcatcacca gcccagagcg tgccgttggg ttcggccgcc atcgcccgga cggaagacgg
      481 ctccaagaca ggcttgaatg aagcgccggc atccgcggat cggatgatgc cgtcctgtcc
      541 gaacgcatat agcgcctcgc tcgtcacgat gagaccgctc gtccagctgt tggtgtgctc
      601 tcccgttaga acgttctcga ggcgaccgtt gtcatcgctg cggtagaaca tgaagcggct
      661 gtttacgaag agtcgctccc catcggaggc gatcgcgacc ggcgagaaca gcgtgttggt
      721 tctcgagttt gcctgtcgga acacctcgac gttgagcttt ggatcggtct tcgtcgcgct
      781 cctgacaacc atcccgcgac ggtctcatgc agctcaggtt cgctacaagc aacagccctt
      841 cgggacggtc gtcgcggaga gcgtgttggg cgaatgggca cacggtgtgc acattttgcg
      901 tttgtgatgc gtggggaaac tgccgacgga agcgacgcga gcatttctgg ctcgctcgcg
      961 gttgggacgc tgcatgggcc gtggcgcatc gagtacgagc tcggccgtgg ggccatgggt
     1021 atcgtctatg ccgtcacgca tgagcacatt ggcaagcgag ccgcgctgaa ggtcctgcat
     1081 cgcacgctcg gcgtcggtac tacaaagtat gccgagcgga tgcttctgga ggcgcgcgtc
     1141 gtgaacgcga tcgcgcatcc gaacatcgtg gacatcttcg atgttggcgc aacggaagac
     1201 ggccggccct acatcgtgat ggagcgcgtc gatggctgcg ctctcgggga cgctgcgctc
     1261 gatcgcgcgg atgcgctcga agtcctggga cagatcgccg atgcgctcgg ggctgcgcat
     1321 gctgttgggg tcgtccaccg cgatatcaag ccggacaata ttctgctcgt gtgcagccgc
     1381 gacggggcgg tctgcgtgaa gctggtcgac tggggaatcg cgcgcatgtt gaacagtgac
     1441 gtgcgccata cgttcgaagg gcagatcgtc gggacgccgc tctacttgag tcccgagcag
     1501 gcgtgcggcg cgccggtgac agcgcagacc gatgtgtact cgcttggcgt cgtcgcgtac
     1561 gagctactgc ttggcgagcc gccgttccgc ggtgcaacac gcgaggtgat ggcgaagcat
     1621 ttgtgggtcg aaccgcgcga tccgcgaacg ctgtggcccg agataccgga cgcgctggcg
     1681 tcgctgttgc tggcgatgct cgcgaaggcg cccgaggcgc ggcccgtgat gacgagcgtc
     1741 gcggcgacga tcgcggccgc gcgtatccgg cgaggcgatg agacgccggc ggaggcgctc
     1801 gaaccgtcgt cgtcggaatc ggagctgcgc gaggccgtgg ctcgtcgagc tctcaggccg
     1861 aatcagatgt tcgcgatcgt cgccggtgcg gcgctcatcg gcgccatcgc tctgatcgtt
     1921 gcggtcacac gtgcgtcgag cgagggccgt gcggtgccga aggtcgctgt gccgagtgtc
     1981 gcggagcggg cgccggcgga agaaccagtc gcgatgccga cggcgcctca ggcgcgaaga
     2041 tcgccggttg tcacgtcgac tcgcgcgacc accgcaccgg cgcagccaag cggtgtcgtc
     2101 gagaattgtc cggatccgag cctgctgcgc gacggtgccg agtgggtgat ggtctgtacg
     2161 ggacgccacg gcgatgacat ctacccgatg cacgtatcgt cggatctgcg ctcgtggcgc
     2221 gacgcggggt tcattttcac cgcggcgacg cggccgacgt gggccgatgg gaactactgg
     2281 tctcccgagg tgcgtcgcac gtccgatgga tatgccgcgt acttcagcat gcgcgtttcc
     2341 ggggcgcgca atgccattgg cgtcgcgacg agtcagtcgg cgcgcggccc tttcgtcgat
     2401 cgcggcgaac cgctgtatgc accgcgagac ggcgccagcg atcccgacgt gttcctcgac
     2461 ggcgggcgaa cattcctgtt tcagcgccgc aacacaccgc atgcgacgct atgggtccag
     2521 gagcttgccg ccgacgcaac gccggtcggc gacgcgcgac aggtgctcgc tccgagcgaa
     2581 gagtgggagc agggcaacgt gcaagttccg gcggtgatac gccgaggcga ctacgtctat
     2641 ttgttctata gcggcgccac gtactgcgat cgaagctatg caattggcgt cgcgcgatcg
     2701 cggtcgccgc tcggtccatt cgagaagatg cggactcccc tcgtgcagtc caacgacgaa
     2761 tggctcggcc cgggccacgc gtcggtcgcg atgacaggga ccggcgagta cgtgatcgcg
     2821 tatcacggct atcgcgcgag cgagggcgag ccgtcatgcg tgcggggagc gaacggcgac
     2881 aacaatgctc ggcacgcacg cgtcgagcgg ctgaccttcg agaatgggtg gccgcgacta
     2941 cgcgattagt tcttcttcgt gtgcctgatc ttgaacgtcc cgtttggcgc gacgcggaac
     3001 tcgcagatct tcgcttcgga tccgcggcgc gtgagctttc cgcgaaacac gtcctcgtag
     3061 tcgccgaggc gatagtgaac ggtgaatggc gcggcgcctc gcccgtgata gtggttgctg
     3121 tagagaacga ccgtgacctt gtagttgccg ggcttggccc ggttgcagac gatgttctcg
     3181 accggatcca cgtcccaggt cttcttgcgg tggttccagc gatcgacgcc aggcatgcga
     3241 tcgacgtcga gcgtaacccc ggcctccttt ttgttgccga agtacacgtc gcccttgggg
     3301 gtgtggcagt ggaggtcgag gtcgacgtga gcgtcccatg ccagcgagaa ctggaactgc
     3361 gtcttgcccg tcttcttgat gatgcggccc ttgttgacgc tcaggcgccc ttggtgctcc
     3421 tcgacggtca tgcggcgaat gaaatctgcc tcgtcccaga tctcgatgcc tttcgcgagc
     3481 gccttgtcga gcttcgagcc ggtcctcgag cccgtgacga tgacgttgca ggcgttggtg
     3541 acggtgctga ccacgctcgc gcccgagcgc tccgccatgg ccttcgcctc ggcgcgggtc
     3601 atggtcagct tgccagtgaa gacgacagtt tttccgtgca gcagcttggg aggcatcaag
     3661 ccgtcgtatc ggaggctcgt acgcgagtca attcgtcagg gtgacagagt tcgcgtggcg
     3721 acgggctcac cgaggcggat gagcgtctcg cgttgctgtg cggtctgctg caggatctcc
     3781 gcgtcggggc gccaccgacc gggctcgccg agcacgacca ccgtcgagcc gccgaacagg
     3841 aagtagccct tctcgtcacc gcgcttgaac gaaggttcga cgtgcgactg gacgatcttg
     3901 ccgaccatca tcgcgccgac ctcgatgtac gcgagcttcc cgaagttagc ggtctcgagg
     3961 atgctgatgt gacgctcgtt ggtggcgagc acgttgctct ttgcctttag cgcgacgggg
     4021 ttcacggagt ggaaggcgcc ttgcgcgcgg tagctgtcga gtacgcgacc gtcgtcgggg
     4081 taatggaagc ggtgatagtc gacgggacac aggcgtgcga gcagcagcgg gccgccgata
     4141 aaatcgcgcg cgtggtcgtc gctgcccatc agggagccgg ctgatagatg ctcgcccttc
     4201 accgggaacg tttggtcgtc ctcgatcgat gtccacgcga gatagcgagc ttccgcgaag
     4261 gccgccatga cgtgcggatc ctgaacgcat ggacgcgcgc cctcgcgaaa tttgcggatg
     4321 aagaagtcgt tgaacgtcgg gaacgggccc ggctcgtact cctccatcgg aatgccgtag
     4381 cgcgcgatga acggctcgac cttgcggcca ctccagcgcg tggattgcag ccagccgtac
     4441 gactgactga accagcgccg tgacaacagc gcgtcgccga ggccgcggcc gaacgggttc
     4501 gcgtacatga accgcacgct tccctcgccg tacaccttct cttcgatctc gcggccgtgc
     4561 ttgcgatccc agacagagat cggtggatgc ttcttgctca cgacctatgc tcgactatag
     4621 caacctgacg agctctgtga acaagaggtc ggaatagctg tgacgattca ggcgccctcg
     4681 ttcgagctgc tgctcgtcgg agatttgctc cccgcgaatg ctgcattcgt gacgctctcg
     4741 cacgatgccg actccgtgac cgtgatgtcc ggggaccgca agatccgggc gtcgccgctg
     4801 tacgccgggc gtgtgacgga cggcaacggc gcgcggtcat acgccatcgt gcacccctcg
     4861 gacgggcggt ggccgaccgg aactctgcgc gtcgtcgcgc gcaaccggca gggcgtcgtt
     4921 gcggaatgca cggtcaccgt ggggccgccg attcacggct cgcaaccgac gtgcacgtac
     4981 gtgagcggcc cgacggaggt cggtactcgc gccggattct tgtatggcgg gctcgcggat
     5041 tcgcacgcgc ccgtcgtcgc gatcatcgtc gacgtcaaga gcggcgaccg ttctcgcacg
     5101 cgagtgttca tcggtgatgc tgtgcgcggt tgcggcggcg tcgcgctcgg cgccttcgac
     5161 gaggtgacgc gcgtccgcat cacgaacgtg ctcggcaact acatcgagct gttcccgccg
     5221 tttcctcatg gcgtcgcgcc ggcggggcca ttcgagtgcg tgtccgacga tctgaatcac
     5281 ttgctctgca acgcggcgta cgagaacgac ttcgaggcgg tgcgtgcggc gctcgccgat
     5341 ggcgccgacg ttcgagcgcg agcttcacgc ggcggctttg ccgcctggac gccgcttgcg
     5401 tgggctgcga cacccgcgat ggccgagctc ctgctcgctg caggcgccga gatcgacgcg
     5461 gaggatgcgg acggatacac gccgatgttt cgcgcgctct tgtacaagcg gctcgacgtc
     5521 gccgacttct tactgcagcg aggcgccgat cccaattact gtgcgtcgcc cgagcacaca
     5581 ctgcccgtcg accaggcgct ccaggtgtcg cccgaagttg ggcttcgagt cctgagagcg
     5641 ggtgggcggg tcggcccgaa gacctacgcc agcctctggc tcaagcagga ctcgcgcgca
     5701 cttctcgaca agtggccgat cctcggtgct ccgatcgcag atcgcgacaa ggagctgatg
     5761 ctcgcagcgc gcagcggacc tctcgacgcg gtgacgacgc tgctgcgcga cggtgcaagc
     5821 gttgcagcgc gcgacacggc aggcgcctct gcactcgatc acgcactgct caaggggaac
     5881 atcgaggccg cgcgcctgct gcaacgcgcg gagctcgcga cgctcgacaa cgcgaagctg
     5941 ttcctcatga cgctggatga ctacgatacg agcctccggg agagagcgtc gtcgttcgat
     6001 ctcgaagcgc gcacgccgcg cggaatgacg gcactgatgc tcgctgcgcg ctatcgccgg
     6061 catcgcgcgc tcggcgcatt gctcgatctc ggggccaaca tcgagcagcg caacggcacg
     6121 tcgacgccgc ttgtcgctgc gatcgagtcc aacaacctct acggcatgct catgctgctc
     6181 gatcgcgggg cggtcatgaa cctccagatc gacggcgcga atcccgtgct gaccgcggcg
     6241 atcaaagcgg acagcgacct gctcaagcac ttgttggccc gcggcctcga ggttccgccc
     6301 gggcaataca ccaagtacga cgagacgggt cgcctgctga cgaatgtgct tgctgggatt
     6361 cggccgtacg tggggccgcc gacgttctcg tcgtggtcgg actgcaagat ctgccgcgag
     6421 ctcccggagc gaatgtactg gtgtaggagc gcgatgggtg aggtcaccgg cgacggcatc
     6481 cctcccatca ccgaacagca gttcgagacc ttcgatcgct atctctggaa atgcccctac
     6541 tgcgcgacct actacgactt cgagcacgat cacgataacg gcctcaccga cggctgggat
     6601 agggacgacc tgacgcggat ctcgctcgac atcgcacgcc agcgcttgga cgcaattcca
     6661 aagcgttcct ccgaattccc ggcgatggct gaggggcgca tcgaacgtga aatcgcgcgc
     6721 gcagcgtttg cgtccgccct cgcgacgccg ggcaaaaagg tccagatcag cctcgacgag
     6781 gtcaaggatg gcaggcgtcc gctgctgatc tacgagctcg cgcctggcgc caagcgcatc
     6841 gcgtggcatc gagtgccgga ctcacaacac gacgtgctcg tcgccgagct gaaggccaga
     6901 ggcctggagc cagcagagac cgactccgac agcatgaatg acttcatctg gatccggacg
     6961 gcgactgggc tcgaggtcta cgacgcgcac cacaaggttc tcgacgtggc tgaagactgc
     7021 gcgacattgc gcgacggaag cgtgatcgtc cgaggtgatg tcgctcgcgt gatcgcatac
     7081 gcgtcgagga actacgtcga gcgcggcgtc aaagtggtgc ttcggtctgg cgtcgagatc
     7141 gacctcgttg cggactactc catctccgcc gagatctact atcagtacaa ccgcaacgac
     7201 ctcctgttcg agaccctctg gtgcgcgcgg atcggccacg ccgtcgcgga ctgggctggc
     7261 atgaagctcg agaatctgat ctaggcgttt acagtgtttc gagctcgcgc agaaccgcga
     7321 gtgcggaggg cggccgcgcg gcgggatcga tcgcgaggca gcgcgcgacg gtcgtgacga
     7381 gctcgggcgc gagcgccggg acgcgttgct cgagcggtac gcgatcggtc gagagcgcga
     7441 gcttgaatgg atcgcccgac gtatcgcggt atgggagggc gcccgacagc atgtggtaga
     7501 gcgtcacgcc gaagctgtag aggtcggtgc ggccgtcgag cggtttgccg tcgcactgct
     7561 ctggtgacat gtacgcaggc gtgccggcga tcgtgtcgtg cgcgctgcgg tagtcgtgaa
     7621 gggattgcgc catgccgaaa tcggtgacgc gcggctgatc gcggcgatcg atgaggacgt
     7681 tgcccggctt gaggtcgcga tggatgaggt ggagctggtg cgctgcctgg agcgcgccga
     7741 gaattccgcg tgcgtaggcg agcgcgcgcg caacgggcag tggtccatcg cggtggagcg
     7801 ttgccgccag cgactcgccg tcgacgagct cgaggctgag gtagtagtgg ccctcgacgc
     7861 tgcggccgaa gtcgtggagc tggacgacgt tcgggtggtg gatccgcgaa gtcgcgtcca
     7921 cttcgcggcg cagctgctcg acggcgccgg gagtttcgag cgattcgggg tggagcagct
     7981 tgatcgctcg cggctcgtgg agcgcgacgt gctgtgcgcg gtagacggtg gccggtccgc
     8041 cgcgaccgat cctcgcctcg atgcgatagc ggccgtcgaa gagctgtccc ggctggagcg
     8101 tcggcggcgc cacgcgcgtc gatggcgtga ggcctttggg cgcaagtgac gtgagcgttg
     8161 cgccgagcgc gagggagtca gccggtggcg gcatgcccgt cgccagcagc gcggcattca
     8221 gcgcggtgtt ctcggtgc
//
LOCUS       DATLBZ010000022        40064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8959748, whole genome shotgun sequence.
ACCESSION   DATLBZ010000022 DATLBZ010000000
VERSION     DATLBZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 40064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40064
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8959748"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VMZ53_00910"
     CDS             <1..904
                     /locus_tag="VMZ53_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS27028.1"
                     /translation="QNAEGARLFEEGRALAKDGKYVEACATFEKSLQLDPAPGTKLNY
                     GDCHEHLGHLAQVYRLFLEVAEADKTANPDRAKFAKSRADALVAKLGTLVINIPDPAA
                     VSSVAIAGRIVPTAGEISEKVDPGDVIVEVKLRSGSPFKQTAAAKAGAAVTIDVPTPA
                     GGGSVIGGGGGGGGGEPSDASQRRHTRLIYAYAAGGVGLASMITGGALGLVARSNYHA
                     QLEGENPNCSNTSPPQCNPEGFSAQQSAITLANVGTGFFVGGIALVGVGAVLFLTAPK
                     ADGDLVVTPTASSESAGLAVVGTF"
     gene            950..2263
                     /locus_tag="VMZ53_00915"
     CDS             950..2263
                     /locus_tag="VMZ53_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HUS27029.1"
                     /translation="MAVKNYVLDTNVLLHDARSLYAFADNNVVIPIFVIEEIDTFKKD
                     QSELGRNARQIARLLDDHRSGTGGLATPQHMKETGGTVRVALSKKEIKNPSYDSRSMD
                     QRILEIALEVRDADPKTPTVLVTKDVNMRVRGDALGLLTADYEPERITIEELYPGNRE
                     LLVPPNVIDEFYANGAVVIDAPGLHANEFLTLKSDDGKSALTRWDKNVGKAVPVKKLR
                     EGVWGIKPRNREQHFALDLLLNDDIKLVTLVGKAGTGKTLVAIAAGLQKVTEEQVFSK
                     MLVSRPVFPLGRDIGYLPGTIEEKLNPWMQPIYDNLELLLGLNKSDKKDGRSYAELVD
                     LGFVEIEPLTYIRGRSLPQNYMIVDEAQNLTPHEVKTIVTRAGEGTKIILTGDPYQID
                     HPYLDSSNNGLTIVAERFKNEAIAGHVILTKGERSPLAELATQIL"
     gene            2260..2658
                     /locus_tag="VMZ53_00920"
     CDS             2260..2658
                     /locus_tag="VMZ53_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27030.1"
                     /translation="MRTLLAILLLSTPAYAETWELKIPERVEVTAGTSGTLPISLAVD
                     RGKTISKDAGIVLDLAPEGGLAVKKRRLARTDAVDPDADAPRFAVPVRADAAGDFTLR
                     VRLRFWVCGQKVCKPVDAKRSVVVAVTAAP"
     gene            2727..3875
                     /locus_tag="VMZ53_00925"
     CDS             2727..3875
                     /locus_tag="VMZ53_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HUS27031.1"
                     /translation="MISRERLVTTGITTAAAATVALVIWLKPWATENIASEQPEPTAT
                     QPAGKPGHMVDIVFAVDTTGSMGGLLDGAKRTVWSIANQVNALDKDADLHVGLVAYRD
                     LGDEYVTKDFALTADLDGMYQELTSYQASGGGDVPENVDAALYDAVHNMKWRKGAKKI
                     IFLVGDAPPASRGEVAKFEQTAKLAAKQQIVINTIRCGQDGETATAWQQIAMLGNGAF
                     STIEQNGGVQQIATPYDDKMAQLAAEIDSTTIIYGSADQRRRYAEKTAAVTSAAPAAA
                     KADRGAYYAKKGGVAAAPAEDVVGGVASGAMNLDDLDEGGLPEEMQNKSKEDLKKDLE
                     ARAAKRSQAQKEIEELAKKRDEYIKANQKDGEVGFDAVVKQTLETQLK"
     gene            3900..5141
                     /locus_tag="VMZ53_00930"
     CDS             3900..5141
                     /locus_tag="VMZ53_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS27032.1"
                     /translation="MQFVELGLIPPIAQAVAGQGYETPTPIQAQAIPHVLAGRDLLGL
                     AQTGTGKTAAFALPILQRLAHISPGRGHRHIRCLVLTPTRELASQITESFTTYGKHLP
                     LRTTVIFGGVGMESQKQALRNGIDILVATPGRLLDLAGQGLVDLKSLEVFVLDEADRM
                     LDMGFIHDVKRVIGLLPSRRQTLFFSATMPREAQELADRLLYKPETVAVTPPSTTAER
                     VEQEAFLVEKADKRALLVEVLRDRSMKRVLVFSRTKHGANRIAEHLEKAGIGAEAIHG
                     NKSQNARERALANFKAGKIRVLVATDIAARGIDIDDVTHVLNFDVPEVPETYVHRIGR
                     TARAGASGMAMTFIEQDEREDWRNIIKLTRQDIPIVEGHPYQSRIPMNAPLPKSNGAS
                     RPQGRSPHSGGHRNGQRRRRR"
     gene            5157..5900
                     /locus_tag="VMZ53_00935"
     CDS             5157..5900
                     /locus_tag="VMZ53_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27033.1"
                     /translation="MVARAAVLTALVLAGCDGVFGLQTIHGDDDDGPREINVGAIEHK
                     HSRETNQFTLPVSTPDGSDMVVLVFAAVGSYCNDSSVPTATLTFNGDTPMPRHVLTGT
                     PCNDALSHSYLWVITDPPTGSHDVAVMLTATAHSLHVGALLLSGVDAMSPVRTVNSAT
                     GVGLASSVMVPSEDGDLVVSFIGQGGNIADPGPGNTLRYLDNDSAATTLNNSGASTIA
                     GSSPQVEARWTFSVDDQWQAIAVSLQPAH"
     gene            complement(5897..6817)
                     /locus_tag="VMZ53_00940"
     CDS             complement(5897..6817)
                     /locus_tag="VMZ53_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27034.1"
                     /translation="MKWFAMCLVLCACRVHFDAPELDAAGDTCGATVPTSGTISGTLA
                     ASTAGDAGSCGGGDVAEAVYSIDVSNGAELLVTADEQGTTADTLIYIRSECMNASTEV
                     VCDTESGMGDNGSYRLTSPTPGRYQIFIDGETAGDFVGRIETLLPAGADCDPANARDR
                     CGAELTCTNQKCSSAACPLADMLTGAATYTRTAITSTAVREHAGSCGDGFDGGVRAPE
                     LIYQLILAAPASNVRVSTESPMTDYDSLIYMRQFTCTGTEVGCDDDTISGAGASQFDT
                     GALPAGDYYIFVDGFGTRSGTAILSVTITP"
     gene            complement(6830..7513)
                     /locus_tag="VMZ53_00945"
     CDS             complement(6830..7513)
                     /locus_tag="VMZ53_00945"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HUS27035.1"
                     /translation="MFSGYKQIVVLTGAGISQAAGLPTYRGPGGLWNDPSKIALSSIE
                     ALRANRAEACAMFWQLYTSAREVKPTAAHRALAAFEAALPPKTDYLLITQNVDGLHQA
                     AGSKRVCEFHGSLARWKCEVCGAQQTLPSEPTHCGEPMRPCAVLFGEEIPAEAEHTAK
                     RALRDCDLFVAIGTSGTVIPAASFVRWAEYAGARRVLLNLEIDNDARGLYSECHAGTA
                     DELVAKYFV"
     gene            complement(7539..8825)
                     /locus_tag="VMZ53_00950"
     CDS             complement(7539..8825)
                     /locus_tag="VMZ53_00950"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS27036.1"
                     /translation="MLDPAVETTTLDNGLSVTTVALPHLHTAVCALAVKVGSRFETPE
                     DNGLSHFVEHMLFRGTERYPTSLALNTAVERLGSTLHAETGRDLSLFQLALEPAQVPS
                     ALDLLGELFGRPRFGEIDLERELILEEINEDYDEHGIEINADDIARGILFENHPLGQR
                     IIGPRTNVERFSNEDIRRHFTTFYGAKNANLCIAGPVEHGRVVEQAARALAAMPVGSR
                     VEVPPAPPAIAGPLLEHVKDTGSQTTLSVVFRGLPELDPAYIALIALLRVLDDGMSTR
                     LHYTLADQKGLAYSISAYIEPLADTALFEITGATANAKVPSLVRELFGLLDGLRRGEV
                     SPDELAKAGVRYRYETLASIDDAAAMAAWFGSTALYYKPPSLSERLEQMSKITVDDVV
                     RVAEQVLVPEKLVVAAVGSLSRARLGELRQVVTDWR"
     gene            8865..9737
                     /locus_tag="VMZ53_00955"
     CDS             8865..9737
                     /locus_tag="VMZ53_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS27037.1"
                     /translation="MLLKKIVIAEDDDAIAHMVSMALGDAGYLCLRARDGDEALKLVK
                     AHDPDLLVLDVMMPRVDGLEVARRLKADVMWSRIPVLMLTALAGVDNEVQGIEAGADA
                     YMSKPFDLREFGAKSKALIRSARRERNRNPTTNLPGSRAIDEEIEGALKSAKPTTVIH
                     IDILNWDSYADAIGIARSEDTVRSLGAWLLEAARQASGGGAFVGHIGGSDFIAVLAPE
                     HADVFINQAESAFDGHRSTLPGDAKNLALTLAIATTEGLALNGGADELGRRLGKAMKS
                     AKAGGKTGHVRFAP"
     gene            9741..11693
                     /locus_tag="VMZ53_00960"
     CDS             9741..11693
                     /locus_tag="VMZ53_00960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27038.1"
                     /translation="MAGDDPRTNKSGARSHDTDPLADTQAPGDTQAEEAASSSSTSKR
                     RSVQAGDVLEDRYELIEDIGEGGMASVYRAKDRQLRREVAIKVLFPHLARREEVVRRF
                     HREARAAAGLEHANILRVYDVGGSDGNEGNDPPYIVMELIRGRSLLGELEQRGPILAE
                     VVACIGALLADALAVAHKAGIIHRDVKPANVMIAPGGRVLLTDFGVARLETEDSLVTK
                     TGALLGTPAYMSPEQAGGDTATAKSDVYSLGATLYQLATGSLPYSGSPAKVMAQIAQG
                     TLVSAVRRRAAVGPDLSRVIDRMMASDPANRPASAADVADELRKIAASGGFGDPKDEL
                     AAYFAEPETFVKERLPKVVASLVTTAERAIADDKLPRAMALADRASALAPDDPAVTQL
                     VHKVTEGSSSMRRRKLLAIIAGGAVLAGGATVGAIQLFGGTHATAPDAALDSRPLDAD
                     TSVADAPTDAVTDALIDSHVIANAPDDAGKPDARPARVKTDAGVVAMAKIDAAVAPPD
                     AAIAVDAAVVEPQFGSILVKNDTFCEVKIDGTSYGRQFPKTAKPIRAAVGRHSVTCEN
                     SGLSSWTKDADVATGKVTTVEGFMLLTVKVTLEVDATIDNTPYTKGSVAVVKPGSHII
                     VAGGSKAWVTIKEACRLRSTPDLGCF"
     gene            11806..12972
                     /locus_tag="VMZ53_00965"
     CDS             11806..12972
                     /locus_tag="VMZ53_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27039.1"
                     /translation="MRSFLAIAAFGLASGCIYVDPINQRPSLDIEREGGGEVFRGDEV
                     RMRALANDPENQLVFFQWRAYACSDATEDESGARPGCDAVPFYTEILQDAKLTVPSMR
                     ADVTEPPQQLLVLLEGQDDFGATAKPIQRLVLAVSDRSPDLALRKDSRYGYVVNTAVN
                     IYAKLGDGDDGPLAALPLEWKVYTPMTQPGYDLVDIDVKQDPMDTKHVQLGKAFTPKG
                     VGDWEIEVTAMDQLQRACVAEGRDDCTKTVERVMITVVPDHAPCLSQLSPLVAAAPNA
                     LPVSDPTLFQVNVVLDDLDPFPGVPSDAVLGTPAFHWSISTNDFATHQDLTTTGNALA
                     FDPASYSPGDIVEVRVEIQDRKNTAVTCADNLATCSTISDNSCIQRQTWRVEVR"
     gene            12972..14741
                     /locus_tag="VMZ53_00970"
     CDS             12972..14741
                     /locus_tag="VMZ53_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27040.1"
                     /translation="MKRFALIALVAGCAGQLDGQSNPDANTSSDAPQGCYATLSYDPP
                     FPVANTITPIRVAVVLSNVSGAGFTYSWTLTRNNNPVTYTQEASDNSAIGFFAPVVGK
                     YSVSVNISGGVPSECSFASAMINVTDPNANNGVVRLRTVPSPTLAPPQETVIQIQGGG
                     DYNRDIALDNGMALAATVTNGVGGPGTAAYVKLMPVSAPAAFTEVFASSTGSFSVRLL
                     PQAHSVLVIPTSTSLAPKLLTWMPVPPVNTFAVSSGTLVTGNVLGPSGTGLVGAKVQL
                     MAGVVPSTLGTTGANGLFSVRTDFPANTPITVRVTPPAGSGLPRLEATGTLNLQQSVQ
                     IAYASTLQTCNLGGNAVKRGGVNQANAAVTFVGALGTAGTATTGAMSVNLAGSVRVAT
                     TANGSGVLPTVVVPRSAALSAVVSLSATDWAVDTVDTSACAAQTIDAPAQITRTGVTK
                     KDATTVLPGVRIEATPVDALAAADAQPVVVTSDSAGAFSIDLASGGTYDVRFIDPLAR
                     AAPLPALGIAPASVPATAVLPKALTISGKVTVTGTSNPIVGASIQILCAGCSGIDASR
                     PIAQTATDAQSNYRIAVPDPGVM"
     gene            14751..15671
                     /locus_tag="VMZ53_00975"
     CDS             14751..15671
                     /locus_tag="VMZ53_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27041.1"
                     /translation="MLRLIVIAWLLAVAAPAHADSVALLPLDGEKRLEIYGQPVASEL
                     AKALNGAGVEVVVVGAKMAVPAQAKLIVDGTIKAKGQTVTLELRIRDARDGSTLESVP
                     PSSGALMNIDKVAAESAARVVPAVKNELEVLAKKAADAAANVAKQPDRDPVPDTTPAG
                     PQEPVVVVGISAQGNGGAAGQLLLSGLRDEISGWAQHHAHTVNPVEPALFDSKNSAPG
                     VVSSNGGEFGMSLHVITLQIWPGRVPMARARVRLRVVSRERIVFDRVIHTDTIVGDPE
                     ISEAQFAARVARDVLRIAHANLKRTTSNWK"
     gene            15668..16435
                     /locus_tag="VMZ53_00980"
     CDS             15668..16435
                     /locus_tag="VMZ53_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27042.1"
                     /translation="MIRAFVVLALVSGVAVAQPAPSADPSAALREANDAATQGDWTKV
                     DGLVQPLLAQQLDRADLAEAHRLAGISALFAQPPRRDLAESHFVAYLKIDLDGHLDPA
                     LYPPDVVNFFNDVRAKHSAELKAIRPKKRYAVLSVLPPFGQFQNGERTKGWVITGVIG
                     TLAIADVTAFLLRRSWCTHVTSPSGNSSYVCDGHYDGARATRTIEIATSLGLLITYVY
                     GVYDGVRGYRRESRERALMPYASPTNQGAVVGVQMNF"
     gene            16572..19619
                     /locus_tag="VMZ53_00985"
     CDS             16572..19619
                     /locus_tag="VMZ53_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27043.1"
                     /translation="MRQPVPFGKYLLLDRISVGGMAEVFKAKSYGVEGFEKIIAIKRI
                     LPTMGEDRDFIKMFIDEAKIAGQLAHANICQIFELGRIDGSHFIAMEYIWGKDLLQIQ
                     NRLRKIKQPMPIPMACFSIAKVLEGLDYAHRKRDPLGRPLEIVHRDCSPQNVLVSYEG
                     EVKVIDFGIAKATSRNSRTMAGVLKGKFGYMSPEQVRGLPLDRRSDIFAIGTMLYECM
                     TGERLFQGETDFSTLEKVRNVDIQAPRSINPLIPEAVEKIILKALAKDVDDRYQWCSE
                     MLADLQAFLMGQDVVFTAKSLSQWLKEVFAQEIERERQLLEQYKRVGRDGLIAGAPAA
                     ESKLNVVEELGEAGVPEGDSTMLGGPDFADMEHGVGATPDVNQSPGGRAGNVNAPLGN
                     KKAPAPQPAPVAAKPAPGYQEDEEGEGDFGEEAPTEIFGDDPAAATPPPAAAARPNVL
                     QPLGKKGPSNPPPAGRPSTQQPAQSPQGMAAAAGGAMQPAVGRVPTGDVAPMGGGYHP
                     PAATPYPSSQPYGTPPGSQPAQGGPGMAMGTPGGMAASGLAQQGDGGEGPQRTVLGVQ
                     APPGLPNYANFRPSQQQPAMNPNMQSYPGYQQPGQTPPSGYGYQQGYPQQGQQGQYPG
                     MPQGMYGSGPMNAMNQSGATPIDQLGGATPMPNEGKKSTLVRDIAIGVGIAALVLVGF
                     LVVKMFVLDKDTTAGNGSAAPVSKLANVKIKMPAGMTAEMEVDGSRVATVENGREIAL
                     SPGKKHIKLTGKDGLTCDDPKVDLEAGKTALLVCGFGQDQGSAAVPNAGSATTDTTGA
                     GSATTQVAQGGTTGTTTTDTKTDVKTTTDTKTDAKADTKTDTKTGTHDVTTTKTDTKT
                     DAKTDTKTGTGTGTGSAATSTTKTDAKTGTTGTTTKTDTKTGTGTTATKTDAKTGTGT
                     STTATKTGTGTTGTTGTDTKTDTKTEAKGDPNKGYLQVFSKPNAKILVDGVDTNLKTP
                     ISGHSLSLAPGKHKITFVIGDDRFTYPVMIKAGQTEQMSKDLQ"
     gene            complement(19713..20318)
                     /locus_tag="VMZ53_00990"
     CDS             complement(19713..20318)
                     /locus_tag="VMZ53_00990"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HUS27044.1"
                     /translation="MPETRIIKRYANRKLYDTEHSRYVTLDQISEMIRNGDDVKIVDN
                     KTKEDLTTVTLAQIIFEEEKKQRSFLPLGAMRNIIQSGGQWFAEAQRRVQSILPGKKA
                     DGESDGQEVAVEETGTPEGDEAAQKKKSVAALREWIEHSKGRLEEWQKQVDSKVRGAL
                     ENVQHAINPWSAVNKDVKALADRIAELEAKLHELEAHEQQK"
     gene            20445..21164
                     /locus_tag="VMZ53_00995"
     CDS             20445..21164
                     /locus_tag="VMZ53_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27045.1"
                     /translation="MRVLALVQALGQGDPVTWVEAMASIIARAHLVDDADAVATVECI
                     THAAADEALPYATRQRLYETATELELPAIARLFLAASPPAEIPHQLKKQLGPERALRP
                     TDRPLTLGERKSAARTHRRDRLLLMIRDPHPAVVSILLDNPHVTEQDVMRMASMRPAV
                     PESLAKIAAHSRWSVRHPVKRALVLNPSTPLADAIRIATTLRAPELEEIANDHSLPDA
                     LRTHAREVLRDVQTRQQRPRA"
     gene            complement(21335..21457)
                     /gene="tatA"
                     /locus_tag="VMZ53_01000"
                     /pseudo
     CDS             complement(21335..21457)
                     /gene="tatA"
                     /locus_tag="VMZ53_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            21545..23533
                     /locus_tag="VMZ53_01005"
     CDS             21545..23533
                     /locus_tag="VMZ53_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HUS27046.1"
                     /translation="MRALAIVALCTSAAHAQPAVSAGPQVIPPVATVGDPSLSQDPDG
                     RRAVRGCSIDEDCGRPRDVLHQFDVEAFPPAGGTPWIDERVTGSRVEPAQVRHVKKPS
                     ELRPDQAWLDKLETPDFPFTWSTQLIDYLLFYKSDPRGRSIMGAWIERQGRYQNLIVS
                     HLRKAHLPEDLQYDAMIESSYDVDDMSSAGALGLWQFMKAAGSIYGLRQDRWVDERMD
                     PLRSTVAQMDYYADLYQRFGNWEIALAAFNVGYGAMLRSIARYNTNDFYQLCAYENGL
                     PWETCLYTPKILAAAIVGRNKAAFGYDTIKQQPAEVWDEVTVPTSMTLSVIARASGAN
                     EADIKKLNPHLKRDRTPPGETGYVVRIPQGAKADFQRKLVELQTEWDGYDAYVVAHGE
                     RLEDVATTYGISLPALRKLNGIARESEIEGGTVIVVPRITEDAREKNKAKAKAKLVAS
                     GVDHKEGDETLIVPVPDKDFTIAGKQRVFYRVVSGDNLDGIAKAFGVKTAQLVEWNGL
                     DEGGKLHPRMVLQAFVAQDFDADKHKIALLDESLIEVVTRGSSEHLDLAEERTGRVRT
                     EYISKGNEKLEEVAKRYGMGSHDLARINRISYKTVLEKGQKIIVYQVADPTRSDRAEE
                     QWKKTPKARRGKTGAKTPPRTSEGPVTKPGQLDEADNQ"
     gene            23530..23868
                     /locus_tag="VMZ53_01010"
     CDS             23530..23868
                     /locus_tag="VMZ53_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27047.1"
                     /translation="MMTRRELIASLASGAALVACGGNSVTPDATASCTDRGTIPEIAQ
                     NHGHVLAITVEDIEAGVEKTYDIMGTSLHTHSVTITAEQFAMLRANQTITVTSTLVTA
                     HSHSVVVKCA"
     gene            23881..24258
                     /locus_tag="VMZ53_01015"
     CDS             23881..24258
                     /locus_tag="VMZ53_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HUS27048.1"
                     /translation="MKRFAVALVMLAACGGDDGSTPMPDAPNMTTNKVQTVTCPTTAD
                     ATVMTTAAVDAYMPATQTVPKNAVVKFVMASTHDVAPNTIGTSDPGLVVGFGATKCLK
                     FTETGTFGFFCSAHSFAGTITVQ"
     gene            complement(24503..25459)
                     /locus_tag="VMZ53_01020"
     CDS             complement(24503..25459)
                     /locus_tag="VMZ53_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27049.1"
                     /translation="MRRTLVVLVLAASATPASADDNDLVLSRLATPVTDGSGNVTGFV
                     GQNLELRSLSSQLGTVLAPHLLTPADTIGFGGFQLTVDYAMTSIDASQPYWRVRNGGA
                     QGGDTMRTVGLFMRKGMWFPLPSFELGGGAVHLVDSKIWTGQLYTKFALHEGYHQLPL
                     PSLSVRGAVSRMMTQRELDLTVASLDVAVSKHFGVGGTWRFDPFVGWNLLMIIPRSEV
                     IDPTPEVDPLVMGNEQDILNNFVFRDQDNIYRNRIFVGAKLQYYVFQLTLEAQFALKG
                     SSKDDRTGTNEACMPNSTTSNCDATDTAATQRTLSMSAGFDF"
     gene            complement(25475..26119)
                     /locus_tag="VMZ53_01025"
     CDS             complement(25475..26119)
                     /locus_tag="VMZ53_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27050.1"
                     /translation="MAGAGVSQNMKRLALVVALAAIACAVWYLRSSHTSVAAKGTPAA
                     KDTPARPSAAPPIDHVTKLSPAERKALAERIASAQATRQPHASPSGAAATHAPAAPRL
                     PDDLPDDDATPISKTQIRDAMREVIPHLADCYDAAIPTLPTPNIDITATITLSGDPDI
                     GTLIDADQVLFKDGTPLPAKFDDCLRTTFMSLALPPLAEGDKIEIHYPFRFAQQ"
     gene            26144..26902
                     /locus_tag="VMZ53_01030"
     CDS             26144..26902
                     /locus_tag="VMZ53_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HUS27051.1"
                     /translation="MYSTIRVDERGPVARITLDRPDKRNPIGPATCGELVAAFGALKA
                     AADVHVVILTGAGTVFSAGGDLSAMQQQTGPTATLVELFTTMHELGKPIIAMVNGHAL
                     AGGLGLMVACDLVIASDQAQFGTTEIAVGLWPMMITAEITRSVGRKKTLEMMLTGRKL
                     NAAEALDAGLINRAVPPDQLETVTMQLATEIAERSPAALKLGLHAFYRSQDMELETQL
                     RYLQAELGRVLALEDAAEGITAFLQKRKPVWKGK"
     gene            complement(26903..27805)
                     /locus_tag="VMZ53_01035"
     CDS             complement(26903..27805)
                     /locus_tag="VMZ53_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27052.1"
                     /translation="MRLAVVVVLATGCRFNFFESDPPRMDVGTSMTDADTNIELGPWG
                     TPVLMTDVSLQVEPDDDPTLTADELEIYFDSGRVVTGGAGGDVWMATRASTADPFNTP
                     TNVTELLSGSDDSTGDLSADGLRLYLSSDRRVTGDRDLYVSSRADRQSAWGTLVRIAE
                     LSATSQNDAGACESSDGLSLIFGSERSGNGDLFVTTRVSTGAPWSLPQLVPGINTAEE
                     ESQHWCNGELTLIYFSRRNATSGDLDIFYASRANPSVPFDPARRVDELTTSGDDADPW
                     VSSDLRTMYFSRGATGGDIFISKR"
     gene            27823..28911
                     /locus_tag="VMZ53_01040"
     CDS             27823..28911
                     /locus_tag="VMZ53_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27053.1"
                     /translation="MRGWIACGVLMLVACGREKQTAQPSAGSAVTAVAVADAHVSDGA
                     APKLAITCGPAMLAPRLDTGKASARKAIAKPPPIPDTRSWTPYAASVGLASDDVAQAA
                     GDELEKLMPLAKLERCFAGKDASVRTMVQVAFDGAVIGARSGGSGDAQVDACISTSLI
                     GLKVTAPPTVSEVACDIVRGDPQPWRVAPESYTVLDVGPTEIKEAGVALTKQTLVPAK
                     ADATHAFLIVVDPAAQPGLVAGAFDRTQWSAATLVALKTESGSPVFIAMGRDGRTREK
                     DYAPLLAIEREKDSVKSCVDGEARGTAPQAEIETLLQTLARSCDPKPCPATLSIALGG
                     TMQDLAAAADAARRAGLPRIGLSTTGCP"
     gene            28929..29771
                     /locus_tag="VMZ53_01045"
     CDS             28929..29771
                     /locus_tag="VMZ53_01045"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HUS27054.1"
                     /translation="MRWAFVVLAIAVATPASARTPTTQTVQKKKTAKAAKAKAAAKAR
                     AKAKKKVATKVAAKHRKAKVKRPRMPKKPLVAVASHGGDGRVRYGRDNMPPGFAWPAT
                     NAMKSATKVCEQTLSDAGIVFEAAPPEGYIVDPIRVPAMEVAGIQLVRAYGKGPVTFD
                     CQLVRVLAKVGPELHQLGVRQIRFGSVYRNTLVRVHGETKPILSRHALGLAMDIKSFV
                     DETGRVADVELDYLKGDSLLHSIEDTVNRSNDFRIVLTPGNDPLSHSDHFHIEASVDY
                     TAFR"
     gene            29781..30287
                     /locus_tag="VMZ53_01050"
     CDS             29781..30287
                     /locus_tag="VMZ53_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUS27055.1"
                     /translation="MADPVVEVATTHGNFVIQLDPAKAPKSVENFLAYVDAKHYDNTI
                     FHRVISTFMIQGGGYDPQMEKKPTRAPITNEADNGLKNKRGTVAMARTSDPHSATAQW
                     FVNVVDNAFLDHTAKDGGGWGYAVFGKVIEGMEVVDEIKGVKTGAAGPFSKDAPEDLV
                     IIKHVRRR"
     gene            30302..32728
                     /locus_tag="VMZ53_01055"
     CDS             30302..32728
                     /locus_tag="VMZ53_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HUS27056.1"
                     /translation="MQSRWLALAVVVAACSDPPPVDVEGGRLTAKIYPEPPRIVLFVD
                     GNEVWSTTGDAGAPYGFGAVGNRAVEIEMQFGSFRFKENKSQESWQGISELSAITLAD
                     TGATFDVRGSDRKLGTGRLTLDSSSLTARIELTVDHADRFSISGECATDEHLVGLGGQ
                     SFDVDHQGETVPLWVQEDGIGKFPDRDDQYLGVWFLTGRKHSTHTPMPMVLSSAGYAI
                     TLDTNARATFDLGGERPGTCRFETWEPHLDLRVFLGEGGEPRDAFSKMINWVGKPATP
                     PELIYAPWIDALFGSANVRRVAQKLRDNGISSSVIWSEDWRGGNYGATGYALEEDWKS
                     DATLYPDFSALASDLHGMGFAFHTYNNTFIDSTADIHNEAVDNGYAIKNGNGDAYSFT
                     GVKFNPSTMLDLSNPAAVAWGKRVMTDGLDAGADGWMADFAEWLPHDAVLASGEDPML
                     VHNRYTVDWARMNKEMFASRPNGIYFMRSAWLHSQPETMVMWAGDQQTDWSEGDGFPS
                     VIPIGLGLGLTGFPYFGSDVGGYMSQGTIPTSEELFYRWTTLGALSPVMRTHHGRSVM
                     ENVQWEHDAGTIAHFRRWSRFHMQLAPYFTGSARGYERDGMPLFRLIALDYPDEDWAW
                     SAVDEYLLGDRILVAPVLAEGTTSRTLKLPAGTWYPLMSGPPVAGGDVTAQASKTEIP
                     AYVPAGSILVLYPDGIDTVLASPALGTAKQPTGAREVWLYSGTAANPAFGTWSDADGP
                     GGADWTWSGRPSGALPSSATFNGSPVSLTMGAEWASVTVSGNGTLAYAGGGTLTIQRG
                     VSATTTVYLR"
     gene            32772..33518
                     /locus_tag="VMZ53_01060"
     CDS             32772..33518
                     /locus_tag="VMZ53_01060"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HUS27057.1"
                     /translation="MRSVLLLVLLAACATRGATPAPHHHVDAPRQAMPKALRAGDLLG
                     VPGESVEYRVAMRGFTLGSVVVAVGQIGEVEGHRAVVVRSRGSGSGAFALFSEISWEL
                     KTVLALDLGKALSEEETIDVELPVGKKEHEHETRDLTADPTHNLHSAAGALRAWRSKL
                     GDTATFDVRVVDAYLTVELTDAAREKHGDMPAVRYDGVAKATGTYKLSIWVSDDETRV
                     PLRFTSQTKWGEVVVDLVSYDVDRALLVNR"
     gene            complement(33508..33957)
                     /locus_tag="VMZ53_01065"
     CDS             complement(33508..33957)
                     /locus_tag="VMZ53_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27058.1"
                     /translation="MPSHPRIRARGTPIEVPIGELTRALCYIPDVFHHTWVEAELALL
                     HITVQTARSVDQLVSALVEDPPPRPQIVFANIGAMSPGELLHLHAIREQGWFGTVFAV
                     GDASLQLRSSLRIERVLQPPLSRSLLRSALLGTSHAQMTTPIRRVNG"
     gene            34022..34897
                     /locus_tag="VMZ53_01070"
     CDS             34022..34897
                     /locus_tag="VMZ53_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27059.1"
                     /translation="MPAEAPETTAEAPPQAPQIGFPKKTLYWIAGVTIVIWAFAISTG
                     NTIFIGIVGVLTAVLAFVMIRAFLFIRKQRGVVDILQGAQGSPEARKEALAKLEQGKD
                     ANSPTTLFARAQLMAADDPKSALQLLDKTDLKAFPPGMQDDVSLLRTQLFLGLGRTAD
                     ARKAADTINLDNPERKAARPLAASIVAEAWARTGKPKEALKLLDSMEVPAKDAEQIGI
                     QAKVARVFARFAANQRGLARNELNSLADDDVNYLGRFLDPRFKVHPELQKLARQVLEK
                     HPAARKVIKQQAQRR"
     gene            34899..35414
                     /locus_tag="VMZ53_01075"
     CDS             34899..35414
                     /locus_tag="VMZ53_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27060.1"
                     /translation="MGALSQAAFEELVNAPCPSCGERALEIHTFIDRSFVVMVGDPND
                     AGRWVHDGEKFVDGTYRIACTKCAHAAFSSDMCPRCNAPHALETALSSTSRVTIPKRC
                     PKCNELELLAIALIPAKAPATTAPKPKPTAEFGEPGYHIVAFACHACDNAVVSERCPL
                     CDAPGPLRPRP"
     gene            35418..35981
                     /locus_tag="VMZ53_01080"
     CDS             35418..35981
                     /locus_tag="VMZ53_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27061.1"
                     /translation="MLPLVFLLVAIAVWWWMSNQPVTVPRVEEVTARIAFPRPLRLDS
                     PDHAIAQLDRPDEIVIPHQYANLVISFPLSKPVTLAIESPIPYGFTRAELVRAICHEY
                     DTIYDIEEATAATKPLPPVLRDNLGRNRTDGLYGIWGHDLSELVCTAIHWTRAADSRV
                     TIRPHVEVQEKPKLDADASPDGDKPAP"
     gene            complement(35994..36845)
                     /locus_tag="VMZ53_01085"
     CDS             complement(35994..36845)
                     /locus_tag="VMZ53_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27062.1"
                     /translation="MALTRIAFASIVLAGCLATPARPRDDAAVADAQRDGAGDSAACA
                     GAWTTPTPLTELTNLVTGEPTITGDKLQLFWSVQNSATSWEIHWASRDAVTDIFSDRG
                     TLPIIDNPSGIDQDPSISDDGLVLMYRVGANEQGAELHEVTRSAATDAWGNPATLAGL
                     TTVTPASLDLSADGLTVYYVTSQTLRSATRGSRTAAFTANGVSLGTMFRFPAISGDEL
                     TLYYEVGTSGSIYRTVRGSTTSAFPAGSVLFNDTNLHDVDVTADETTLVVATRDGNTI
                     AISTRCP"
     gene            complement(36836..37687)
                     /locus_tag="VMZ53_01090"
     CDS             complement(36836..37687)
                     /locus_tag="VMZ53_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS27063.1"
                     /translation="MSEQTLALDIYRKGNDEFVQSRYSQALVLYREAVVHWDHPAIRY
                     NMAVCLIEMNEPLEAFEMLERALKFGEAPLGAEKLEKGKAYRKELLGQLAQLDITCTT
                     ERSGVTLDGQPLLACPGKVQRVVAPGRHVIVGRREGFIPYTQDFTAVAGATTTYDVTL
                     SPLAAEPSKQPPPLDVVAESQPRSRLGLYVIAGGAALGVVALTYDLLAVQPARKDLTQ
                     SAQAFDDGVSAFRTRRNIDVAMFAAAGVAVGVGVYLHVKRSGDRDALVVAPSVGSHSA
                     MVTVQWH"
     gene            complement(37789..38970)
                     /locus_tag="VMZ53_01095"
     CDS             complement(37789..38970)
                     /locus_tag="VMZ53_01095"
                     /inference="COORDINATES: protein motif:HMM:NF006102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HUS27064.1"
                     /translation="MPRKVNVIGVGMVPFQKPGKSEEYHVMASKAGKAALDDAKVAFT
                     EVQQAFAGYVYGDSTCGQRAIYTLGLTGIPVFNVNNNCSTGSSALMLGAQAVSGGNAE
                     CVIVVGFEQMEAGALQAKWTDRANPLDKFVNVMNEEQGFNQAPPAAQMFGGAGREYRW
                     KYGTKKETFGKIAEKARLHASKNPYALFKETYSLEQIMQSPEVFDPLTRYQCCPPTCG
                     AAAAVLCSDEFAKKHGISNPVYMAGQVMTTDYPSTFEEHSMIKMVGFDMAKNAAKKLY
                     EQTGIGPEDVDVVELHDCFTANELLTYEAIGLCKEGEAEKFIWDGNNTYGGKYVTNPS
                     GGLLSKGHPLGATGLAQCTELVWHLRGTAENRQVEGAKIALQHNLGLGGACVMTMYKR
                     D"
     gene            complement(38970..>40064)
                     /locus_tag="VMZ53_01100"
     CDS             complement(38970..>40064)
                     /locus_tag="VMZ53_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HUS27065.1"
                     /translation="SADIFRAIGGFVAGNPATAEKVKTTFLFKLNSPDSTWTIDLSTP
                     PGAVHDSAVGKAACTLEISDADFMAMATGQADAMKLFSTGKLKISGDVMASQKLGFLK
                     KITPEMVLAETKKRSGGASQAAAASVPAAYTPTVDDAFEVFGEVIKQNPEVVQKVGVV
                     YGFKVGSKSFIVDLKAGKVSTGEGAAECTLELAESDFLDLTQGKADPMKLFTTGKLKI
                     SGNVMASQKLQSLFKIDPSKAIETVMARRGGGGGAAAAPAAAAPAAAKASSDPNAPKF
                     FAALEKRLSENKALAQEVRAKVTFNVTGPDATKTFELGGDTAATLTIADSDLPALASG
                     NVKSLYQHGKLKVDGDVTVAHRLGFLKGAI"
BASE COUNT         7182 a        13022 c        13272 g         6588 t
ORIGIN      
        1 gcagaatgcc gagggtgcac gactgttcga agagggccgc gcactcgcga aagacggcaa
       61 gtacgtcgaa gcgtgcgcga cgttcgagaa gagcctgcag ctcgatcccg cgcccggcac
      121 gaagctgaac tacggcgact gtcacgagca cctcggtcac ctcgcgcagg tgtaccggct
      181 gttcctcgaa gtggccgagg ccgacaagac ggcgaatccc gatcgcgcga agttcgctaa
      241 gagccgcgct gatgcgctcg tcgcaaagct cggcaccctc gtcatcaaca ttcccgatcc
      301 ggctgccgtc tcgtccgtcg cgatcgcagg ccgcatcgtg ccgaccgccg gcgagatctc
      361 cgaaaaggtc gatccgggcg atgtcatcgt cgaggtgaag ctgcggagcg gctcgccgtt
      421 caagcagacc gccgcagcga aggccggtgc agccgtgacg atcgacgtcc ccacgcccgc
      481 gggcggtggc agcgtcatcg gcggtggcgg gggcgggggc ggcggagagc cgagcgacgc
      541 ctcgcagcgc cgccacacgc gcctcatcta tgcgtacgca gccggcggcg tcggcctggc
      601 gtcgatgatc accggtggcg cgctcggcct cgtggcccgc agcaactacc acgcgcagct
      661 cgagggcgag aatccgaact gctcgaacac ctcgccacca cagtgcaatc ccgagggctt
      721 ttccgcgcaa cagagcgcga tcacgctcgc aaacgtcggg acgggattct tcgtcggagg
      781 aatcgcgctc gtgggcgtcg gagccgtgct atttctgact gcgccaaagg cggacgggga
      841 tctcgtcgtc acgcccacgg cctcgtcgga gtcggccgga ctcgccgtcg tcggtacgtt
      901 ctgagccacg ctcgttgaca cggaagccgc gcaatcggat ggtcgtcagg tggcggtcaa
      961 gaattacgtc ctcgacacga acgtgctcct ccacgacgca cgctcgctgt acgcgttcgc
     1021 cgataacaac gtcgtcatcc cgatcttcgt gatcgaagag atcgacacct tcaagaagga
     1081 tcaatcggag ctgggccgca acgcgcgtca aatcgcacgt ctgctcgacg atcaccgcag
     1141 tggcaccggc ggtctcgcga cgccgcagca catgaaggag acgggcggca ccgttcgcgt
     1201 cgcgctgtcg aaaaaagaga tcaagaaccc gagctacgac tcgcggtcga tggaccagcg
     1261 catcctcgag atcgcactcg aggtccgcga cgcagatccc aagacgccga cggtcctcgt
     1321 cacgaaggac gtcaacatgc gcgtccgtgg cgacgcgctc ggcctgctca cggcggacta
     1381 cgagccggag cgcatcacga tcgaagagct gtatccgggc aaccgcgagc tgctcgttcc
     1441 gccgaacgtc atcgacgagt tctacgcaaa cggtgcggtc gtcatcgatg caccgggact
     1501 gcacgcgaac gagttcctca cgctcaagag cgacgacggc aagagcgcgc tgacccgctg
     1561 ggacaagaac gtcggcaaag cggtgccggt gaagaagctg cgcgaaggcg tgtggggcat
     1621 caagccgcgc aaccgcgagc agcacttcgc gctcgacctc ttgctcaacg acgacatcaa
     1681 gctcgtcacg ctcgtcggca aggccggcac cggcaagacg ctcgtcgcga tcgcggcggg
     1741 cctgcagaag gtcaccgagg agcaggtctt ctcgaagatg ctggtctcgc gcccggtgtt
     1801 cccgctcggt cgcgacatcg gctatctgcc gggcaccatc gaagagaagc tgaacccgtg
     1861 gatgcagccc atctacgaca acctcgagct gctcctcggc ctcaacaagt ccgacaagaa
     1921 ggacggtcgc agctacgcag agctcgtcga cctcggcttc gtcgagatcg agccgctcac
     1981 gtacatccgc ggccgctcat tgccgcagaa ctacatgatc gtcgacgagg cgcagaacct
     2041 cacgccgcac gaggtgaaga cgatcgtgac gcgcgcaggt gaaggcacga agatcatcct
     2101 gacgggcgac ccctatcaga tcgatcatcc gtacctcgac tcgtcgaaca acggcctcac
     2161 gatcgtggca gagcgcttca agaacgaagc gatcgcaggt cacgtgatcc tgacgaaggg
     2221 cgagcgctcg ccactcgcgg agctcgcgac ccagatcctg tgaggacgct gctcgcgatc
     2281 ttgctgctgt ccacgcctgc gtacgcagag acgtgggagc tcaagattcc cgagcgcgtc
     2341 gaggtgaccg caggcacgag cggcacgctg ccgatctcgc tcgccgtgga tcgcggcaag
     2401 accatctcga aggacgcggg catcgttctc gatctcgcgc ccgaaggcgg actcgccgtg
     2461 aagaagcgcc ggctcgcgcg caccgacgcg gtcgatccag atgccgatgc gccgcggttc
     2521 gccgttccag tgcgcgcgga tgcggccggc gatttcacgc tgcgcgtgcg gctgcgcttc
     2581 tgggtctgcg ggcaaaaggt ctgcaaaccc gtcgacgcca agcgcagtgt ggtcgtcgca
     2641 gtcaccgcag cgccgtaagt ccttgggtcg caaaaacacg tagttgcgat tagatcgggc
     2701 ggcaaggtcg tgcgtggaac acaggcatga tcagccgtga gcgcctcgta actactggaa
     2761 tcacgaccgc cgccgccgcc accgtggctc tcgtcatctg gctcaagccg tgggcgaccg
     2821 agaacatcgc gagcgagcag cccgaaccaa ccgcgacgca gccggcaggc aagcccggcc
     2881 acatggtcga catcgtgttc gcggtcgata cgaccggctc gatgggtggc ctgctcgatg
     2941 gcgcgaagcg caccgtgtgg tcgatcgcga accaggtcaa cgctctcgac aaggatgccg
     3001 acctccacgt cggtctcgtc gcgtatcgcg atctcggcga cgagtacgtc acgaaggact
     3061 tcgcgctcac cgcagatctc gacggcatgt atcaagagct cacgagctat caggcatcgg
     3121 gcggtggcga cgttcccgag aatgtcgacg cggcgctcta cgacgctgtt cacaacatga
     3181 agtggcgcaa aggcgcgaag aagatcatct tcctcgtcgg tgatgcgccg cctgcgagcc
     3241 gcggcgaggt cgcgaagttc gagcagactg cgaagctcgc cgcgaagcag cagatcgtga
     3301 tcaacacgat tcgctgtggc caggacggcg agaccgccac cgcgtggcag cagatcgcga
     3361 tgctcggcaa cggcgcgttc tcgacgatcg agcagaacgg cggcgtccag cagatcgcga
     3421 cgccgtatga cgacaagatg gcgcagctcg ccgcggagat cgattcgacg accatcatct
     3481 acggctcggc ggatcagcgc cgccgctacg cggagaagac cgcggcggtg acgtcagcgg
     3541 ctccggctgc ggcgaaggcg gatcgcggcg cgtactacgc gaagaagggc ggcgttgcgg
     3601 cggctccggc cgaggacgtc gtcggtggcg tggcgtcggg ggcgatgaat ctcgacgacc
     3661 tcgacgaggg cggtctcccc gaggagatgc agaacaagtc gaaggaagac ctcaagaagg
     3721 acctcgaggc ccgtgccgcg aagcgcagcc aggctcagaa ggaaatcgag gagcttgcga
     3781 agaagcgcga cgagtacatc aaggcaaatc agaaggatgg cgaggtcggt ttcgacgcgg
     3841 tggtcaagca gacgctagag acgcagctca agtagggatt ttgtgctacg cccccgcgaa
     3901 tgcagtttgt agagctgggg ttgatcccgc cgatcgcgca ggcggtcgcg gggcagggct
     3961 acgagacacc gacgccgatc caggcacagg cgattccaca cgtcttggcc gggcgcgatc
     4021 tgctcggtct cgcgcagacg ggaaccggca agaccgccgc gttcgcgctg ccgattctcc
     4081 aacgtctcgc gcacatttcg ccgggtcgcg gccaccgaca cattcgctgc ctcgtgctga
     4141 cgccgacgcg cgagctcgcg tcgcagatca cggagagctt caccacgtac ggcaagcacc
     4201 tgccgctgcg caccacggtc atcttcggtg gcgttggcat ggagtcgcag aagcaggcgc
     4261 ttcgcaatgg catcgatatc ctcgtcgcca cgccgggtcg cctcctcgat ctcgccggcc
     4321 aaggcctcgt cgatctgaag agtctcgaag tgttcgtgct cgacgaagcg gatcgcatgc
     4381 tcgacatggg gttcatccat gacgtgaagc gtgtgatcgg gctgctgccc tcgcggcgcc
     4441 aaacgctgtt cttctcggcg acgatgccgc gcgaagcgca ggagctcgcg gatcgcctgc
     4501 tttacaagcc ggagactgtc gcggtaacgc cgccgtcgac caccgccgag cgcgtcgagc
     4561 aagaagcatt tctcgtcgag aaagccgaca agcgcgcgct gctcgtcgag gtgttgcgcg
     4621 accgatcgat gaagcgcgtg ctcgtgttct cgcgcacgaa gcacggcgcg aaccgcatcg
     4681 ccgagcacct cgagaaggcc ggcatcggcg ccgaggcgat ccacggcaac aagtcacaga
     4741 atgctcgcga gcgcgcactc gcgaacttca aggcgggcaa gattcgcgtg ctcgtcgcga
     4801 cggacatcgc ggcgcgcggc atcgatatcg acgatgtcac ccacgtcctc aacttcgacg
     4861 tgcccgaggt ccccgagacg tacgtccatc gcatcggtcg caccgcacgc gcaggcgcat
     4921 cgggcatggc aatgacgttt atcgagcaag acgagcgcga ggactggcga aacatcatca
     4981 agctaacgcg ccaagacatc ccgatcgtcg agggccatcc gtaccagtcg cgcattccga
     5041 tgaacgcgcc gctgccgaaa tcgaatggcg catcgcgtcc gcaaggtcgc tcgccgcaca
     5101 gtggcggtca ccgcaacggc cagcgccgcc gccgccgctg agataccatc gcgtagatgg
     5161 tcgctcgcgc cgccgtgctg actgcgctcg tgctcgcggg ctgcgatggc gtgttcggct
     5221 tgcagacgat tcacggtgac gatgatgatg gtccgcgcga gatcaacgtc ggagccatcg
     5281 agcacaagca ctccagggag accaaccagt ttacgttgcc ggtgagcacc ccggacggca
     5341 gcgatatggt cgtgctcgtg tttgccgcgg tcggcagcta ctgcaacgac agctccgtgc
     5401 cgaccgcgac gttgacgttc aacggtgaca cgccgatgcc gcgtcacgtg ctgacgggta
     5461 cgccatgcaa cgatgcgctc tcgcattcgt atttgtgggt catcactgat ccgccgacgg
     5521 ggagccacga cgtcgcagtc atgttgaccg cgaccgcgca cagtctccac gttggcgcgc
     5581 tgctgctcag cggtgtagac gcgatgtcgc cggtgcggac cgtcaatagc gcgaccgggg
     5641 tggggctcgc atcgtccgtc atggttccga gtgaagacgg cgatctcgtc gtgagcttca
     5701 tcggtcaggg cggcaacatc gccgaccctg gtccgggaaa cacgctgcgc tacctggaca
     5761 acgacagtgc ggcgacgacg ctcaacaata gtggcgccag cacgattgcg ggtagcagcc
     5821 cgcaggtgga ggcacgctgg acattctcgg tggacgacca gtggcaggcc atcgcggtat
     5881 cgctccagcc ggcgcactag ggcgtgatcg tgacactcag aatcgctgtc cccgagcgtg
     5941 tgccaaaacc gtcgacgaag atgtagtaat cgcctgcggg cagcgcgccg gtatcgaact
     6001 gcgacgcgcc ggcgccggag atcgtgtcgt catcgcaacc gacttcggta cccgtgcacg
     6061 tgaattggcg catgtagatg agcgagtcgt aatccgtcat cgggctctcc gtggagacgc
     6121 gcacgttgct ggccggagcc gcgaggatca gctggtagat gagctcgggt gcgcgaaccc
     6181 cgccgtcaaa cccgtcgccg caagaaccag cgtgttcgcg caccgccgtc gacgtgatcg
     6241 ccgtacgcgt gtacgtggcc gctccggtca gcatatcggc gagcgggcat gcggcgctcg
     6301 agcacttctg gttcgtgcac gtcagttcgg ctccgcagcg gtcgcgcgca ttggctggat
     6361 cgcaatcagc gccggccggt agcaaggttt cgatccgacc aacgaagtca ccggcggtct
     6421 ctccgtcgat gaagatctga taacgacccg gcgttggtga tgtcagccga tacgagccgt
     6481 tgtcgcccat tccggactcc gtatcgcaga cgacctcggt cgaggcgttc atgcactcgg
     6541 agcggatgta gatcagcgtg tcggcagtgg tgccctgttc gtcagcggtg acgaggagct
     6601 ctgcaccatt gctcacgtcg atcgaataga cggcttccgc aacatcgcca cccccgcacg
     6661 agccggcgtc gccggcggtg ctggccgcga gcgtgccgct gatggttccg ctcgtcggta
     6721 cggtggcccc acatgtgtcg cccgccgcgt cgagctcggg cgcgtcgaag tgcacgcggc
     6781 acgcgcaaag cacgaggcac atcgcgaacc acttcacgcg cgatcgattc tagacgaagt
     6841 atttcgcgac aagctcgtcg gcggtgccgg cgtgacactc ggagtacaag ccgcgcgcgt
     6901 cgttgtcgat ctcgagattg agcagcacgc gtcgcgcgcc cgcgtactcg gcccagcgca
     6961 cgaagctcgc cgcggggatc acggtgcccg acgtgccgat cgcgacgaac agatcgcagt
     7021 cgcgcagcgc gcgcttcgcc gtgtgctccg cctcggcggg aatctcctcg ccgaataaaa
     7081 cggcgcaggg ccgcatcggc tcgccgcaat gcgtgggctc tgacggcagc gtctgctgcg
     7141 cgccgcacac ttcgcatttc cagcgcgcga ggctgccgtg gaattcgcag acgcgcttgc
     7201 tacccgcggc ttggtgcagg ccgtcgacat tctgcgtgat gagcagatag tcggtcttcg
     7261 gcggcagtgc ggcctcgaat gcggcgagcg cgcgatgcgc cgcggtgggc tttacctcgc
     7321 gtgcgctcgt gtagagctgc cagaacatcg cgcacgcctc ggcgcggttt gcgcgcagcg
     7381 cttcgatgct cgacagcgcg atcttcgacg ggtcgttcca gagcccgccg gggccgcgat
     7441 aggtgggaag ccccgccgcc tgcgagatgc cggcgcccgt gagcacgacg atctgcttgt
     7501 accccgagaa catccactca gtgtaacagc aacactcttt agcgccagtc ggtcacgact
     7561 tggcggagct cgccgaggcg ggcgcgcgac agcgagccga cggcagcgac gacgagcttc
     7621 tccgggacga gcacttgctc ggcgacgcgg acgacatcgt cgacggtgat cttcgacatc
     7681 tgctcgagcc gttccgagag agagggcggc ttgtagtaga gggcggtcga gccgaaccag
     7741 gcagccatcg ccgccgcgtc gtcgatcgag gcgagcgtct cgtaccgata acgaacgccg
     7801 gccttcgcga gctcgtcagg cgagacctcg ccgcgccgca ggccatcgag cagaccgaac
     7861 agctcgcgca cgagcgacgg gacctttgca ttcgccgtcg cgccggtgat ctcgaacagc
     7921 gccgtatcgg cgagtggctc gatgtacgcc gagatcgaat aggcgaggcc tttctgatcg
     7981 gcgagcgtgt agtgcaggcg cgtcgacatg ccgtcgtcga ggacgcggag cagcgcgatt
     8041 agcgcgatgt acgcggggtc gagctcgggg aggccgcgaa atacgaccga gagcgtcgtc
     8101 tgcgagccgg tatctttgac gtgctcgagg agtgggcccg cgatcgcggg cggtgcgggt
     8161 ggcacctcga cgcgcgagcc gaccggcatc gccgcgagag cgcgagcggc ttgctcgacg
     8221 acgcgaccgt gttcgacagg gccggcaatg cagaggttcg cgttcttcgc gccgtagaac
     8281 gtggtgaagt ggcggcggat gtcttcgttc gagaagcgct cgacgttcgt gcgcggtccg
     8341 atgatgcgct gaccgagcgg atggttctcg aacaggatgc cgcgcgcgat gtcgtcggcg
     8401 ttgatctcga tgccgtgctc gtcgtagtcc tcgttgatct cttcgaggat cagctcgcgt
     8461 tcgaggtcga tctcgccgaa ccgcgggcgg ccgaacagct cgccgagcag gtcgagtgcg
     8521 gacggaacct gggccggctc gagggccagc tggaacaggg ataggtcgcg cccggtctcg
     8581 gcgtgcaggg tcgacccgag ccgctcgacc gcggtgttca gtgccagcga cgtcgggtag
     8641 cgctcggtgc cccgaaacag catgtgctcg acgaagtgcg agagcccgtt gtcctcggga
     8701 gtctcgaacc gagaccccac tttgacggcc agcgcgcaaa cggccgtgtg gaggtggggc
     8761 agggcgaccg tcgtgaccga cagcccgtta tcgagcgtcg tcgtctctac ggccggatcg
     8821 agcacttatt caggcttacc atgtggtatc aaagggtcga tccgatgctc ctcaaaaaga
     8881 tcgtgatcgc ggaggacgac gacgcgattg ctcacatggt gagcatggct ctcggagacg
     8941 cgggctactt gtgcttgcgc gcccgcgatg gtgacgaggc gttgaagctg gtcaaagcgc
     9001 acgaccccga cttactcgta ctcgacgtca tgatgccgcg cgtggacggc ctcgaagtgg
     9061 cgcgccggct gaaggccgac gtcatgtggt cgcgcatccc ggtgctgatg ctcacggcgc
     9121 tcgcgggcgt cgacaacgag gtgcagggca tcgaagccgg cgccgacgcg tacatgtcca
     9181 agccgttcga cctgcgtgag ttcggcgcga agtcgaaggc gctgatccgg tccgcccgtc
     9241 gcgagcgcaa tcgcaacccc acgaccaacc tgcccgggtc gcgcgcgatc gatgaagaga
     9301 tcgaaggcgc gctcaagagc gccaagccga ccacggtcat ccacatcgac atcctcaact
     9361 gggattcgta cgccgacgcg atcggcatcg cccgctctga agacaccgtg cgcagccttg
     9421 gcgcgtggct cctcgaagcg gcgcggcagg cctccggtgg cggcgcattc gtgggtcaca
     9481 ttggtggctc ggacttcatc gcggtgctcg cgcccgagca cgccgacgtg tttatcaatc
     9541 aggcggagtc cgcattcgac ggtcatcgct cgacgttgcc gggcgatgcg aagaacctcg
     9601 cgctgaccct cgcgatcgcg acgaccgaag gcctcgcgct caacggcggt gccgacgagc
     9661 tcgggcggcg cctcggcaag gcgatgaaga gcgcgaaggc aggcggcaag accggccacg
     9721 tccggttcgc gccgtaaccc gtggctggcg acgacccccg aacgaacaag tcgggcgctc
     9781 gcagtcacga caccgatcca ctcgccgaca cgcaggcgcc aggcgacacg caagccgaag
     9841 aggctgcgtc gtcctcatcg accagcaagc ggcgttcggt tcaagctggc gacgtcctcg
     9901 aggatcgtta cgagctcatc gaagacatcg gcgaaggcgg catggcatcg gtgtatcgcg
     9961 cgaaggatcg gcagctccgg cgcgaagtcg cgatcaaagt gctgttcccg catctggcgc
    10021 gccgcgaaga ggtcgtgcgt cgctttcatc gcgaagcgcg cgccgccgca ggtctagagc
    10081 acgcgaacat cctgcgcgtt tacgacgtcg gtggctccga cggcaacgaa ggcaacgacc
    10141 cgccatacat cgtcatggag ctcatccgcg gccgctcgct gctcggtgag ctcgaacaac
    10201 gcggcccgat cctcgccgag gtcgtcgcgt gtatcggcgc gctgctcgcc gatgcgctcg
    10261 cggtcgcaca caaggccggc atcatccatc gcgacgtgaa gccggcaaac gtcatgattg
    10321 cgcccggtgg tcgcgtgctg ctcaccgatt tcggtgtcgc gcggctcgag acagaagact
    10381 cgctcgtgac gaagacaggc gcgctgctcg gcacgccggc gtacatgagc cccgagcaag
    10441 cgggcggcga caccgctacg gcgaagagcg atgtctactc gctcggcgcg acgctttatc
    10501 agctcgcgac cggctcgctt ccgtacagcg gctcgcccgc gaaagtcatg gcgcagatcg
    10561 cgcaaggcac gctcgtgtcg gcggttcgtc gacgcgctgc ggtcggtccg gatctgtcgc
    10621 gcgtgatcga tcgcatgatg gcgtcggatc cggcgaatcg cccagcgagt gcggccgacg
    10681 tcgcggacga gctgcgcaag atcgcggcga gcggtggctt cggcgatccc aaagacgagc
    10741 tagccgcgta cttcgcggag cccgagacgt tcgtcaaaga gagattgccg aaggtcgtcg
    10801 catcgctcgt gacgacggca gagcgcgcga tcgccgacga caagctgccg cgcgcgatgg
    10861 ctctcgccga tcgagcaagt gcgctcgcgc ccgacgaccc agctgtcacg cagctcgtgc
    10921 acaaggtcac cgagggctcg tcgtcgatgc gccgtcgcaa gctgctcgcg atcatcgcgg
    10981 gcggtgccgt gctcgcaggt ggcgcgacgg tcggcgcgat acagctgttc ggcggcacgc
    11041 atgccaccgc gcccgacgct gcgctcgact cgcgcccgct cgatgcggac acgtcggtcg
    11101 ccgacgcgcc gaccgacgcc gtgaccgatg cgctgatcga ctcgcacgtg atcgcgaacg
    11161 cgcctgacga tgccggcaag ccggacgcac gacccgcccg cgtgaagact gacgccggcg
    11221 tcgtcgcgat ggcgaagatc gatgccgccg tcgcgcctcc cgatgccgcg atcgccgtcg
    11281 atgccgccgt tgtcgagccg cagttcggtt cgatcctcgt gaagaacgac acgttctgcg
    11341 aggtgaagat cgacggcacg tcgtacggac gtcagttccc gaagactgca aagccgattc
    11401 gtgcggcggt cggccgtcac tcggtgacct gcgagaactc gggattgagc tcgtggacga
    11461 aggacgccga cgtcgccaca ggcaaagtca ccaccgtcga gggcttcatg ctcttgaccg
    11521 tgaaagtcac gttggaggtc gacgcgacga tcgacaacac gccgtacacg aaggggtccg
    11581 tcgccgtcgt gaagccgggg tcgcacatca tcgtcgcggg cggcagcaaa gcctgggtga
    11641 ccatcaagga ggcctgccgg cttcgaagta ccccggacct cggctgtttc taggatctcc
    11701 gcgatccgcg acacaggtgt cgtgatcgcc gccgccgctt cgtgagacgc tctacatatt
    11761 cggggactta cgcctggcag agccgttgca aaacgaggag ccgtcttgcg gtcgttcctc
    11821 gccatagctg cgttcgggct cgcttcgggc tgcatctatg tcgacccgat caaccagcgt
    11881 ccctcgctcg atatcgagcg ggagggcggt ggcgaggtct tccggggaga cgaagtgcgg
    11941 atgcgcgcgc tcgcgaacga tcccgagaac cagctcgtgt tcttccagtg gcgcgcgtac
    12001 gcgtgcagcg acgcgacgga agacgagagc ggtgcgcgcc cgggctgtga tgcggtgccg
    12061 ttctacacgg agatcctgca ggacgcgaag ctgacggtgc cgagcatgcg tgccgatgtg
    12121 acggagccgc cgcagcagct gctcgtgctg ctcgaaggtc aggatgattt tggcgcgacg
    12181 gccaagccga ttcagcggct cgtgctcgcg gtcagcgatc gctcgccgga tctcgcgctg
    12241 cgcaaggaca gccgctacgg ctacgtcgtc aacacggcgg tgaacatcta cgcgaagctc
    12301 ggcgacggcg acgatggtcc gctcgcggcg cttccactcg agtggaaggt ctatacgccg
    12361 atgacgcagc cggggtacga cctcgtcgac atcgacgtga agcaggatcc gatggacacc
    12421 aagcatgtcc agcttggcaa ggcgttcacg ccgaagggcg tcggcgattg ggagatcgaa
    12481 gtcaccgcga tggatcagct gcagcgcgcg tgcgtcgcgg aaggtcgcga tgactgcacg
    12541 aagaccgtcg agcgcgtgat gatcacggtc gttccggatc acgcgccgtg cctgtcgcaa
    12601 ctctcgccgc tcgtcgccgc cgcgccgaac gcgctgccgg tgtcggatcc gacgctgttc
    12661 caggtcaacg tcgtgctcga cgatctcgat ccgtttccgg gcgtgccgtc ggacgcggtg
    12721 ctcggaacgc cggcgttcca ctggtcgatt tcgacaaacg attttgcgac gcatcaagat
    12781 ctgacgacga ccggcaatgc tctcgcgttc gatcccgcga gctactcccc cggcgatatc
    12841 gtcgaggtgc gcgtcgagat ccaagatcgc aaaaacacgg cagtgacgtg cgccgataat
    12901 ctcgcgacgt gctcgaccat cagcgacaac agctgcatcc aacgccaaac gtggcgcgtg
    12961 gaggtgcggt gatgaagcgg tttgctctca tcgcgctggt cgcgggttgc gcaggccagc
    13021 tcgacggcca gagcaatccc gacgccaaca cgagtagcga cgcgcctcag ggttgctatg
    13081 cgacgctctc gtacgatccg ccgtttccgg tcgcgaacac gatcacgccg attcgcgtgg
    13141 cggtggtgtt gtcgaacgtg tcgggcgctg gcttcacgta ctcgtggacg ctgacgcgaa
    13201 acaacaaccc cgtcacgtac acgcaagagg cgagcgacaa ctcggcgatc ggcttcttcg
    13261 cgccggtcgt cggcaagtac agcgtctccg tcaacatcag cggcggtgtg ccatcggagt
    13321 gcagcttcgc ctcggcgatg atcaacgtca ccgatccgaa cgcgaacaac ggcgtcgttc
    13381 gtctgcgcac cgttccgtcg ccgacgctcg cgccgccgca ggagaccgtc atccagatcc
    13441 aaggcggcgg cgactacaac cgcgacatcg cgctcgacaa cgggatggcg ctcgccgcaa
    13501 cggtgaccaa tggcgtcggc gggcctggca ctgcggcgta cgtgaagctg atgcccgtct
    13561 cggcgccggc tgcattcacc gaagtgttcg cgtcgagcac gggctcgttc tcggtgcgct
    13621 tgctgccaca agctcacagc gtcctcgtga tcccgacgtc gacctcgctc gcaccgaagc
    13681 tgctgacgtg gatgcccgtg cccccggtca acacgtttgc ggtcagcagc ggcacgctcg
    13741 tcaccggcaa cgtgctcggc ccgtcgggca cggggctcgt cggcgcgaag gtgcagctca
    13801 tggccggcgt cgtaccctcg acgctcggga ccaccggcgc caacggcttg ttctccgtgc
    13861 gcaccgattt cccggcgaat acgccgatca ccgtgcgcgt gaccccgccg gccggcagcg
    13921 gccttccgcg tctcgaggcc acgggcacgc tcaatctcca gcagagcgtg cagatcgcgt
    13981 acgcgtcgac gctgcagacg tgcaacctcg gcggcaacgc cgtgaagcgc ggcggcgtga
    14041 atcaggcgaa tgcggccgtc acgttcgtcg gcgcgctcgg caccgcaggc accgcgacga
    14101 ccggcgccat gtccgtcaac ctcgcgggct ccgtgcgcgt tgcgacgacg gcgaatggca
    14161 gcggcgtgtt gccaacggtc gtcgtgcctc gctcggccgc gctctccgcg gttgtctcgc
    14221 tctcggcgac agattgggcc gtcgacaccg tcgacacgag cgcatgcgct gcgcagacga
    14281 tcgacgcgcc cgcgcagatc acgcgcaccg gcgtgacgaa gaaagatgcg accaccgtgc
    14341 tgccaggcgt gcgaatcgaa gcgacgccgg tcgacgcgct cgccgctgcg gatgcgcagc
    14401 ccgtcgtcgt cacgtccgat agcgccggcg cgttctcgat cgatctcgca tcgggcggca
    14461 cctacgacgt gcgcttcatc gatccactcg cacgcgcggc gccgcttccc gcgctgggca
    14521 tcgcaccggc gagcgtgcca gcaaccgcgg tcctgccgaa ggcactgacg atctccggca
    14581 aggtcaccgt gaccggcaca tcgaatccga tcgtcggcgc atcgatccag atcctgtgtg
    14641 cgggatgcag cggcatcgac gcctcgcggc cgatcgccca gaccgcgacc gacgcacaga
    14701 gcaactaccg catcgccgtt cccgaccctg gcgtgatgta ggatctcctc gtgctgcggc
    14761 tgatcgtcat cgcgtggttg ctcgctgttg ccgcgcccgc gcacgccgat tcggtcgcgc
    14821 tgcttccact cgacggcgag aaacggctcg aaatctacgg ccagccggtc gcgtcggagc
    14881 tcgcgaaggc gctcaacggc gcaggcgtcg aggtcgttgt cgtcggcgcg aagatggcgg
    14941 tgccggcgca ggcgaagctc atcgtcgacg gcacgatcaa agcgaagggc cagaccgtca
    15001 cgctcgagct gcgcatccgc gacgcgcgcg atggctcgac gctcgagagc gtgcctccgt
    15061 cgagcggtgc gctcatgaat atcgacaagg tcgcggcaga gtccgcagcg cgcgtcgtgc
    15121 ccgcagtgaa gaacgagctc gaggtcctcg cgaagaaggc cgcggacgcc gccgcaaatg
    15181 tcgcgaagca gccggatcgt gatccggttc ccgacacgac gcccgcgggg ccgcaggagc
    15241 ccgtcgtcgt cgtcggcatc tccgcgcaag gcaatggtgg agccgccggg cagctgctgc
    15301 tctcggggct gcgcgacgaa atttccggct gggcccagca ccacgcgcat accgtcaatc
    15361 ccgtcgagcc cgcactgttc gactcgaaga actcggcacc gggcgtcgtc tcgtcgaacg
    15421 gcggcgaatt cggcatgtcg ctgcacgtca tcacgttgca aatctggccg ggccgcgtgc
    15481 cgatggcgcg tgcgcgagtg cggcttcgcg tggtctcgcg cgagcgcatc gtgttcgacc
    15541 gcgtgatcca cacggacacg atcgtcggcg atccggagat cagcgaggcg cagtttgcgg
    15601 cacgtgtcgc gcgcgacgtc ctgcgtatcg cccacgcaaa tctcaagcgc acgacgagca
    15661 actggaaatg atccgcgcgt tcgtcgtgct ggcgctcgtg agcggcgtcg cggtggcgca
    15721 gccggcacca tcggccgatc cgtcggcggc gctccgcgaa gccaacgatg cggcgacgca
    15781 aggcgactgg acgaaggtcg acgggctcgt ccagccgctg ctcgcgcaac agctcgaccg
    15841 ggccgacctc gccgaagcgc atcgtctcgc cggcatctcc gcgctgttcg ctcagccgcc
    15901 gcgccgtgac ctcgccgagt cgcacttcgt cgcgtatctg aagatcgacc tcgatggcca
    15961 tctcgatccc gcgctgtacc cgcccgacgt cgtgaacttc ttcaatgacg ttcgcgcgaa
    16021 gcactccgcc gagctcaaag cgatccgccc gaagaagcgc tacgcggtgc tctccgtcct
    16081 gccgccgttc gggcaattcc aaaacggcga gcgcacgaag ggctgggtca tcaccggcgt
    16141 gatcggcacg ctcgcgatcg cagatgtaac ggcgttcctc ttgcgacgga gctggtgcac
    16201 gcacgtaacg tcgccgtcgg gaaactccag ctacgtgtgc gacggtcact acgacggtgc
    16261 acgagctaca cgcacgatcg agatcgcgac atcactcggc ctcctgatca cctacgtgta
    16321 tggcgtgtac gacggtgtcc gcggctatcg gcgcgaatca cgagagcgcg cactcatgcc
    16381 gtacgcaagt ccaacgaacc aaggcgccgt cgtcggcgtg cagatgaatt tctgatctcg
    16441 tcgggggctg caaaccagag tctggagtcc actgcacgag cgagcagacg atcgttgtcg
    16501 tcggtcggcc ggggtttata ggcctcttcg tcattgagat ccggcgttgg cgccggtacg
    16561 atttctttca ggtgaggcag cccgtcccgt ttgggaaata ccttctgctc gaccgcatca
    16621 gcgtcggcgg aatggccgag gtcttcaaag cgaagagcta cggcgtcgag ggcttcgaga
    16681 agatcatcgc gatcaagcga atcctgccga cgatgggcga ggaccgcgac ttcatcaaga
    16741 tgttcatcga tgaagcgaag attgcgggac agctcgcgca cgcgaacatc tgccagatct
    16801 tcgagctcgg tcgcatcgac ggctctcact tcatcgcgat ggagtacatc tggggcaagg
    16861 atctgctcca gatccaaaac cggctgcgca agatcaagca gccgatgccc atcccgatgg
    16921 cgtgcttctc catcgcgaag gtcctcgagg gtctcgacta cgcgcatcgc aagcgcgatc
    16981 cgctcggccg tccgctcgag atcgtgcacc gcgactgctc gccgcagaac gtgctcgtct
    17041 cgtatgaggg cgaggtcaag gtcatcgact tcggcatcgc gaaagcgacg agccgtaact
    17101 cgcgcacgat ggccggcgtg ctcaagggca agtttggcta tatgtcgccc gagcaggtcc
    17161 gcggcctgcc gctcgatcgc cgcagcgaca tcttcgcgat cggcacgatg ctctacgagt
    17221 gcatgaccgg cgagcgcctg ttccaaggcg agaccgattt ctcgacgctc gagaaggttc
    17281 gcaacgtcga catccaagcg ccgcgctcga tcaatccgct gattccggaa gcggtcgaga
    17341 agatcattct caaggcgctc gcgaaagacg tcgacgatcg ttaccagtgg tgcagcgaga
    17401 tgctcgccga cctgcaggcg ttcctgatgg gccaggacgt cgtgttcacg gcgaagtcgc
    17461 tctcgcagtg gctcaaagaa gtgttcgcgc aggagatcga gcgcgagcgc cagctgctcg
    17521 agcagtacaa gcgcgtcggt cgcgacggcc tcatcgcggg cgcgccggcc gcagaatcga
    17581 agctcaacgt cgtcgaggag ctcggggaag caggggttcc cgagggcgac tcgacgatgc
    17641 tcggaggtcc cgatttcgcg gacatggagc acggggttgg ggcgacgccc gacgtcaacc
    17701 agagtccggg gggacgcgct ggtaacgtca atgcgccgct tggcaacaag aaggcgcccg
    17761 cgccgcaacc ggcgcccgtc gcggcgaagc ccgcccccgg ctaccaagag gacgaagaag
    17821 gcgaaggcga tttcggcgag gaagcaccga ccgagatctt cggcgacgat cctgctgctg
    17881 ccacgccgcc gcccgccgct gccgcgcgcc cgaacgtgct gcagccgctc ggcaagaagg
    17941 gtccatccaa cccgccgcca gctggtcgcc cgagcacaca gcagcccgcg cagtctccgc
    18001 aaggaatggc cgcagctgcg ggtggtgcga tgcagcccgc ggtcggtcgc gtgccgacgg
    18061 gcgatgttgc gccgatgggc ggtggctatc acccgccagc ggcgacgccg tatccgagct
    18121 cgcagccgta tggaacgccg cccggctcac agccggcgca gggtggcccc ggcatggcga
    18181 tgggcacgcc cggcggaatg gccgcgagtg gcctcgctca gcagggcgat ggcggcgaag
    18241 gtccgcagcg caccgtcctc ggcgtgcaag cgcctccggg tctgccgaac tacgccaact
    18301 tccgtccgtc gcagcagcag cctgcgatga acccgaacat gcagtcgtac ccgggctatc
    18361 agcagccggg gcagacgccg ccgagtggct acggctatca gcaaggctat ccgcaacagg
    18421 gccagcaagg tcagtacccg ggcatgccgc agggcatgta cggctcgggc ccgatgaacg
    18481 cgatgaatca gtcgggcgcg acgccgatcg atcagctcgg cggcgcaacg ccgatgccga
    18541 acgagggcaa gaagtcgacg ctcgtccgcg atatcgcgat cggtgtcggc atcgctgcgc
    18601 tcgtgctcgt tggattcctc gtcgtgaaga tgttcgtgct cgacaaggac acgaccgccg
    18661 gcaacgggtc ggcagcgccg gtctcgaagc tcgcgaacgt gaagatcaag atgcccgcgg
    18721 gcatgacggc cgagatggaa gtcgacggct cgcgcgtcgc caccgtcgag aacggccgcg
    18781 agatcgcact gtcgcccggc aagaagcaca tcaagctgac gggcaaagac ggcctgacct
    18841 gcgacgatcc caaggtcgat ctcgaagccg gcaagaccgc gctgctcgtg tgcggctttg
    18901 gccaagacca gggcagcgcc gcggtgccga acgcagggtc ggcgacaacc gacacgacgg
    18961 gtgcgggtag cgcgacgacg caagtcgccc aaggcggcac gaccggtacg acgacgacgg
    19021 acacgaagac cgacgtcaag acgacgacgg atacgaagac cgacgcgaag gccgatacga
    19081 agacggatac gaagaccggc acgcatgacg tgacgacgac gaagaccgat acgaagaccg
    19141 acgcgaagac cgatacgaag acgggcacgg gcacgggcac cggctcggcc gcgacatcga
    19201 cgacgaagac ggatgcgaag accggcacga ccggcacgac gacgaagacg gatacgaaga
    19261 cgggcacggg cacgaccgcg acgaagaccg acgcgaagac gggcacgggc acgagcacga
    19321 ccgcgacgaa gaccggcacc ggcaccacgg gcacgaccgg cacggacacg aagacggata
    19381 cgaagaccga ggcgaagggc gacccgaaca agggctacct gcaggtcttc agcaagccga
    19441 acgcgaagat cctcgtcgac ggcgtcgaca cgaacttgaa gacgccgatc tcaggtcact
    19501 cgctctcgct cgcgcctggc aagcacaaga tcacgttcgt gatcggcgac gaccgcttca
    19561 cgtatccggt catgatcaag gctggtcaga ccgagcagat gtcgaaagac ctgcagtaag
    19621 tcgcctgcgc ctccatggcg ccgctgactc cggacgctaa ccgctagatt gtccgaatac
    19681 cggacaatcg cgcctcgcgc gcggagcgct gcttactttt gctgctcgtg cgcttcgagc
    19741 tcgtgcagct tggcttcgag ctcggcgatg cgatcggcga gagctttgac gtctttgttc
    19801 acggcggacc acggattgat cgcgtgctgg acgttctcga gcgcgccgcg gactttggag
    19861 tcgacttgct tctgccactc ttcgagccgg cccttcgagt gctcgatcca ttcgcggagc
    19921 gcggcgacgc tcttcttctt ttgcgcggcc tcgtcaccct cgggggtacc cgtctcctcg
    19981 acggcgacct cttgcccgtc cgactcgccg tcggccttct tgccggggag gatcgactgg
    20041 acgcggcgct gcgcttcggc gaaccactgg ccgccggact ggatgatgtt gcgcatcgcg
    20101 ccaagcggga ggaagctgcg ctgcttcttc tcttcttcga agatgatctg cgcgagcgtg
    20161 accgtcgtca gatcctcttt ggtcttgttg tcgacgattt ttacgtcgtc gccattccgg
    20221 atcatctcgc tgatctggtc gagcgtgacg taccggctgt gctccgtgtc gtagagcttc
    20281 cgatttgcgt agcgcttgat gatccgtgtt tcgggcatga cgcgctcctc aatgaattca
    20341 aagatatcac gcagcgcgcc atgacctcaa cgtgccgtgt cacactgaag ctcgtggggc
    20401 cagacctcat catcgggcgc ctcaaggcga tacgggagcc gcggatgcgg gtgctggcgc
    20461 tggtccaggc cctggggcag ggcgatcccg tcacctgggt cgaggcgatg gcgagcatca
    20521 tcgcgcgggc ccatctcgtc gacgatgcgg acgccgttgc gacggtcgag tgcatcaccc
    20581 atgcggcggc cgacgaggca cttccatatg cgacgcgcca gcggctctac gagaccgcga
    20641 ccgagctcga gctgccggcc atcgcgcggc tgtttctggc ggcctcacca ccggcagaga
    20701 tccctcacca gctgaagaag cagctcggac cggaacgcgc gctgaggccg acggatcggc
    20761 cgctgacgct gggcgagcgc aagtcggcgg cgcgcacgca tcggcgcgat cggctgctgc
    20821 tcatgattcg cgatccgcat ccggcggtcg tctcgatcct cctcgataat ccgcacgtca
    20881 ccgagcaaga cgtcatgagg atggcttcga tgcggccggc ggtgccggag tcgctcgcga
    20941 agatcgcggc gcactcgcgc tggtcggtgc ggcatcccgt gaagcgcgcg ctcgtcctca
    21001 atccgtcgac gccgttggcg gacgcgatcc gcatcgcgac gacgctgcgc gcaccggagc
    21061 tagaagagat cgcgaacgat cattcgctgc cggacgcgct gcgcactcat gcgcgcgaag
    21121 tcttgcgaga tgttcagacg aggcagcagc ggccacgcgc ctagggggcc gcgccgcgcc
    21181 tctacgcttc tttcttcttc ttgcggcgga cgacgacctc ttcccattcg ccgtcgtcgc
    21241 tctcgccagc cgacagctcc ttcttggagg tcgcggcctc gatctgaccc ttgccgcccg
    21301 ccgcctcgat gaccttgtca tcgccgtcga cgttggaggc tttcttgaaa ttcttgatac
    21361 ttcgccccaa cgcatcgccg atacccggca gcttattagc gccgaacacg acgaggacga
    21421 tgaggaggat gacgacgagc tcgcccattc cgggcatgga ggtctccttt ggcttcgccc
    21481 aatgtagcag aaaagccgcg cctaaaactg gttccgccga ggatgggcac ctatgttcag
    21541 gtccatgcgc gcgctcgcca tcgtggcact ttgcacgagt gcggctcatg cccagccggc
    21601 tgtgagcgca gggcctcaag tcattccgcc cgtcgcgact gttggcgatc cgtcgctctc
    21661 tcaagatcca gacggccggc gtgcggtgcg aggctgcagc atcgacgagg actgcggtcg
    21721 cccacgcgat gtgctgcacc aattcgacgt cgaagcgttt ccgccagcgg gcggcacgcc
    21781 gtggatcgac gagcgcgtga caggaagtcg cgtcgaaccc gcacaagtcc gtcacgttaa
    21841 aaagcccagc gagctgaggc ccgatcaggc gtggctcgac aagctcgaga cgcccgactt
    21901 cccattcacg tggtcgacgc agctcatcga ctacttgctc ttctataaga gcgatccgcg
    21961 cggccgctcg atcatgggcg cgtggatcga gcgccagggc cgctatcaga atctgatcgt
    22021 ctcgcacctg cgcaaggcgc acttgcccga ggatctccag tacgacgcga tgatcgagtc
    22081 gtcgtacgac gtcgacgata tgtcctcggc aggcgcgctc ggcctgtggc agttcatgaa
    22141 ggccgccggc tcgatctacg gccttcgcca agatcgctgg gtcgacgaac gcatggatcc
    22201 gctgcgctcg accgtcgcgc agatggacta ctacgcggat ctctatcagc gcttcggcaa
    22261 ctgggagatc gcactggcgg cgttcaacgt cggctacggc gcgatgctgc gatcgatcgc
    22321 tcgctacaac acgaacgact tctaccagct gtgcgcgtac gagaacggcc tgccctggga
    22381 gacgtgcctc tacacgccga agatcctcgc cgccgcgatc gtcggtcgca acaaggccgc
    22441 gtttggctac gacacgatca agcagcagcc agctgaagtc tgggacgagg tcaccgtgcc
    22501 gacgtcgatg acgctgtcgg tgatcgcgcg cgcatcaggc gcgaacgaag cggacatcaa
    22561 gaagctcaac ccgcatctca agcgcgatcg cacgccacct ggcgagactg ggtacgtcgt
    22621 gcgcatcccg caaggtgcga aggcggactt ccagcgaaag ctcgtcgagc tgcagaccga
    22681 atgggacggc tacgacgcgt atgtggtcgc gcatggcgag cgcctcgaag acgtcgcgac
    22741 gacgtacggc atctcgttgc ccgcgctgcg caagctcaac ggcatcgcac gcgagagcga
    22801 gatcgaaggc ggcaccgtca tcgtcgtgcc tcgcatcacc gaagatgcgc gcgagaagaa
    22861 caaggcaaag gcgaaagcca agctcgtcgc atccggtgtc gatcacaaag agggcgacga
    22921 gacgctgatc gtcccggtgc ccgacaagga cttcacgatc gccggcaagc agcgcgtgtt
    22981 ctatcgcgtc gtcagcggcg acaacctcga cggcatcgcg aaggcgttcg gcgtaaagac
    23041 cgcgcagctc gtcgagtgga atggcctcga cgagggcggc aagctgcacc cgcgcatggt
    23101 gctgcaagca ttcgttgcgc aggacttcga cgccgacaag cacaagatcg cgctgctcga
    23161 cgaatcgctc atcgaggtcg tcacacgcgg ctcgagcgag catctcgatc tcgccgagga
    23221 gcgaaccggc cgcgtgcgca ccgaatacat ctcgaagggc aacgagaagc tcgaggaagt
    23281 cgcgaagcgc tacggcatgg gctcgcacga tctcgcacgc atcaaccgca tctcgtacaa
    23341 gacggtcctc gagaagggcc agaagatcat cgtctaccag gtcgccgatc cgacgcgcag
    23401 cgatcgcgcc gaagagcagt ggaagaagac accgaaggca cgccgcggca agaccggcgc
    23461 gaagacgccg ccgcgcacgt ccgaaggtcc ggtcacgaag cccgggcagc tcgacgaggc
    23521 cgacaaccag tgatgactcg ccgagagctc atcgcgtcgc tcgctagcgg cgcggctcta
    23581 gtcgcgtgcg gcggtaacag cgtcacgccc gatgcgaccg cgagctgcac cgaccgcggc
    23641 accatccccg agatcgcgca gaaccatggc cacgtgctcg cgatcaccgt cgaggacatc
    23701 gaggccggcg tcgagaagac ctacgacatc atggggacgt cgctccacac gcactccgtc
    23761 acgatcacgg cagagcaatt cgcgatgctg cgtgcgaatc agacgatcac cgtcacatcg
    23821 acgctcgtga ccgcgcactc gcattccgtc gtcgtgaagt gcgcgtgata tggtcggccc
    23881 atgaagcgat tcgcagtagc gctggtgatg ctcgccgctt gcggcggcga cgacggttcg
    23941 accccgatgc ccgacgcacc gaacatgacc acgaacaagg tgcagaccgt cacctgcccg
    24001 acgacggctg acgccaccgt catgaccaca gccgccgtcg acgcgtacat gcccgcgacg
    24061 cagacggtcc cgaagaacgc cgtcgtgaag ttcgtgatgg cgtcgacaca cgacgtcgcc
    24121 ccgaacacga tcggcacgag cgatccgggc ctcgtcgtcg gcttcggcgc gacgaagtgc
    24181 ctgaagttca ccgagacggg tacgttcgga ttcttctgct cggcgcacag cttcgccggc
    24241 acgatcaccg ttcagtagtc gcgtaagcgt aagcgagcaa tcgccgcttg ctggtggcgt
    24301 tgtcaccgct gccgtcccgc ggcgtgtgct gcggcgtgtg ctcgaagcgg cgtgtgatcg
    24361 acgcggcatg tgctcgaaag cggcatgtgc tcgcgtgctc gaagcggcgt gtgcttgaaa
    24421 cggcgtgtgc tcgtgtgctc gaagcgggcg cgtgctcgag gcggcgtgtg cttcggctta
    24481 cgcttacgct tacgcctacg gctcagaagt cgaagccagc ggacatcgac agcgtgcgct
    24541 gcgtggcggc ggtgtcggtc gcgtcgcagt tcgacgtcgt cgagttcggc atgcacgctt
    24601 cgttcgtgcc ggtgcgatcg tccttcgagc tgcccttgag tgcgaactgc gcctcgaggg
    24661 tgagctggaa cacgtagtac tggagcttcg ctccgacgaa gatgcggttg cgatagatgt
    24721 tgtcctgatc gcggaacacg aagttgttca ggatgtcctg ctcgttgccc atgacgaggg
    24781 gatcgacctc gggtgtcgga tcgatgacct cggagcgagg gatgatcatg agcaagttcc
    24841 agccgacgaa cggatcgaac cgccacgtac cgccgacgcc gaagtgcttc gagaccgcga
    24901 cgtcgagcga tgcgaccgtc aagtcgagct cgcgctgcgt catcatgcgc gacaccgcgc
    24961 cgcgaacgga gaggcttgga agcggtagct ggtggtagcc ctcgtggagc gcgaacttcg
    25021 tgtagagctg gcccgtccag atcttcgagt cgacgaggtg gacggcgccg ccgccgagct
    25081 cgaacgaggg gagcgggaac cacatgccct tgcgcatgaa caggccgacg gtgcgcatcg
    25141 tgtcgccccc ctgcgcgccg ccgttgcgaa cgcgccagta cggctggctc gcatcgatcg
    25201 aggtcatcgc gtagtcgacg gtcagctgga agccgccgaa gccgatcgtg tcggcgggcg
    25261 tgagcaagtg cggcgctagc accgtgccga gctggctcga cagagagcgg agctcgaggt
    25321 tctggccgac gaagccggtg acgttgccgc taccgtcggt gacgggcgtc gcgagacgcg
    25381 agagcacgag atcgttgtcg tcggcagatg ccggcgttgc actggccgcg agcaccagga
    25441 cgacgagcgt gcgtcgcatg atgcgagcgt aacatcactg ctgcgcgaat cggaatgggt
    25501 agtggatttc gatcttgtcg ccttcggcaa gcgggggcag cgcgagcgac atgaaggtcg
    25561 tgcgcaggca gtcgtcgaac ttcgccggca ggggcgtgcc atccttgaac aggacctggt
    25621 cggcgtcgat gagggtcccg atgtcggggt cgccggacag ggtgatcgtg gcggtgatgt
    25681 cgatgttcgg ggtcggcaag gtcgggattg ctgcgtcgta gcagtccgcg agatggggaa
    25741 tgacctcgcg catcgcatcg cgaatctgcg tcttcgagat cggggttgcg tcgtcgtccg
    25801 gcaggtcgtc gggcaggcga ggtgccgcgg gcgcatgggt ggctgccgca ccggacggcg
    25861 aggcgtgggg ctgccgcgtg gcctgtgccg atgcgattcg ttcagccagt gctttgcgct
    25921 cggccggcga gagcttcgtg acgtgatcga tcggaggcgc cgcggagggc ctggccggcg
    25981 tgtccttcgc tgcgggcgtg cccttcgccg ccacgctcgt gtgggacgag cgcagatacc
    26041 aaaccgcaca tgcgattgcc gcgagggcga cgacgagggc caagcgcttc atgttctgcg
    26101 agacacccgc gcctgccatc cgttgcatgt tacacaaccc ggcatgtatt cgacgattcg
    26161 cgtcgacgag cgcgggccag tcgcccgtat cacgctcgat cgccccgaca agcgaaaccc
    26221 gatcggaccc gcgacgtgtg gcgagctcgt cgctgcattc ggcgcgctca aggccgctgc
    26281 tgatgttcac gtcgtgatcc tcaccggtgc gggcacggtg ttctccgcgg gcggcgacct
    26341 ctcggcgatg cagcagcaga ccggaccaac ggcgacgctc gtagagctgt tcacgacgat
    26401 gcacgagctc ggcaagccga tcatcgcaat ggtcaacggt catgcgctcg ccggcggcct
    26461 cggcctcatg gtcgcgtgcg acctcgtaat cgcgtcggac caagcgcagt tcggcacgac
    26521 ggagatcgcc gtgggcctgt ggccgatgat gatcaccgcc gagatcacgc gctcggtcgg
    26581 ccgcaagaag acgctcgaga tgatgctgac cggccgcaag ctcaacgccg cagaagctct
    26641 cgacgccgga cttatcaatc gcgcggtgcc acccgaccag ctcgagacgg tgacgatgca
    26701 gctcgcgacc gagattgcag agcgcagtcc cgccgcgctg aagctcggct tgcacgcgtt
    26761 ctatcgctcg caggacatgg agctcgagac gcagctgcgc tacctgcaag cggagcttgg
    26821 tcgcgtgctc gcactcgaag atgccgccga gggcatcacg gcgtttctgc agaagcgcaa
    26881 gcccgtgtgg aagggcaagt agtcagcgct tcgagatgaa gatgtcgccg ccggtcgcgc
    26941 cgcgggagaa gtacatcgtt cgcagatcgg agctgaccca cggatcggca tcgtcgccgc
    27001 ttgtcgttag ctcgtcgacg cgccgtgcgg gatcgaacgg cacgctcggg ttcgcgcgcg
    27061 acgcgtagaa gatgtcgagg tcaccgctcg tggcgttgcg gcgcgagaag tagatcaacg
    27121 tcagctcgcc gttgcaccag tgctggctct cctcctcggc cgtgttgatg cctggcacga
    27181 gttgcggaag cgaccacggt gcgccggtcg acacgcgcgt cgtgacgaat aagtcgccgt
    27241 tgccgctccg ctctgacccg aagatgagcg acaggccatc gctgctctcg catgcgccag
    27301 catcgttttg gctcgtggcc gagagctcgg cgatgcgcac gagggtgccc cacgcggact
    27361 ggcgatcggc gcgcgagctc acgtagaggt cgcggtctcc ggtaactctg cggtcgctgc
    27421 tgagatacag gcgaaggccg tcggccgaaa gatctccggt gctgtcgtcg gagcccgaca
    27481 agagctcggt gacgttggtc ggtgtgttga aaggatctgc ggtcgaagcg cgcgtcgcca
    27541 tccacacgtc gccaccggcg ccgcccgtca cgacgcgccc gctgtcgaaa tagatctcga
    27601 gctcgtcggc ggtgagcgtt ggatcatcgt cgggctctac ttgaaggctc acgtcggtca
    27661 tcaagaccgg tgttccccat ggaccgagct cgatgttcgt gtcggcgtcg gtcatcgacg
    27721 ttccgacgtc catgcgcggt ggatcgctct cgaagaaatt gaaccggcag ccggtcgcga
    27781 gcacaacaac tacggccagc cgcacccaca tatactaggc cgatgcgggg ctggatcgcg
    27841 tgtggagtgc tgatgctcgt ggcctgtggc cgcgagaagc agaccgcaca accgtcggcg
    27901 ggctcggctg tgacagctgt cgctgtggcg gacgcgcacg tctccgatgg tgcagccccg
    27961 aagctcgcga tcacctgtgg ccctgcgatg ctcgcgccgc ggctggacac cggcaaagcg
    28021 agcgcacgaa aggccatcgc gaagccgccg ccgattccgg atacgcgatc gtggacgccg
    28081 tacgccgcgt cggtcgggct cgcaagtgac gacgtcgcgc aagcggccgg tgacgagctc
    28141 gagaagctga tgccgctcgc gaagctcgaa cgatgtttcg cgggcaagga cgcgtccgtg
    28201 cgcacgatgg tgcaagtcgc gtttgacggc gcggtgatcg gcgcgcgcag cggtggcagt
    28261 ggcgacgcgc aggtcgatgc atgcatctcg acgtcgctga tcgggctgaa agtcaccgcg
    28321 ccgccgacgg tgagcgaagt cgcgtgtgac atcgtacgcg gcgatccgca gccgtggcgc
    28381 gtcgcgccgg agagctacac cgtgctcgat gtcgggccga cggagatcaa agaggccggt
    28441 gtcgcgctga cgaagcagac gctcgtgcct gcgaaggcgg atgccacgca tgcgtttctg
    28501 atcgtcgtcg atcctgcggc gcagcccggt cttgtcgcgg gcgcgttcga ccgcacgcaa
    28561 tggagcgcgg caacactcgt cgcattgaag acggagagcg gctcgccggt attcatcgcg
    28621 atgggccgcg acggccgaac acgcgagaaa gactacgcgc cgctgctcgc gatcgagcgc
    28681 gagaaggaca gcgtgaagtc gtgcgtcgac ggtgaggcgc gcgggacggc gccgcaggcc
    28741 gagatcgaga cgctgctaca gacgctcgcg agatcgtgtg atcccaaacc atgtccagcg
    28801 acgttgtcga tcgcacttgg cggaaccatg caagatctcg cggctgccgc cgacgcagcg
    28861 cgccgtgcgg gattgcctcg catcggtttg tccacgaccg gttgcccata gcctgctagc
    28921 ctcgaagcat gcgttgggcg tttgtggtgc tggcgatcgc ggtggcaacg cccgcgtctg
    28981 ctcgcacgcc gacgacgcaa accgtccaga aaaagaagac ggcgaaggcc gcgaaggcga
    29041 aggcggcggc gaaggcgagg gcgaaggcga agaagaaagt cgccacgaag gtcgccgcga
    29101 agcatcgcaa ggcgaaggtc aaacgaccgc gcatgccgaa gaagccgctc gtcgcggtcg
    29161 cgtcgcatgg tggcgacggt cgcgtgcgtt acggccggga caacatgccg ccgggcttcg
    29221 cgtggcccgc gacgaacgcg atgaagtcgg cgacgaaggt ctgcgagcag acgctctccg
    29281 acgcgggcat cgtgttcgaa gcggcgccgc ccgagggcta catcgtcgat ccgatccgcg
    29341 tgcccgcgat ggaggtcgcc ggcatccagc tcgttcgtgc ctatggcaaa ggcccggtca
    29401 cgttcgattg ccagctcgtt cgcgtgctcg cgaaggtcgg tccagagctg caccagctcg
    29461 gcgtgcgcca gatccgcttt ggcagcgtct atcgcaacac gcttgtccgg gtgcacggcg
    29521 agaccaagcc gatcttgtcg cgtcacgcgc taggtctggc catggacatc aagtcgttcg
    29581 tcgacgagac gggcagggtc gcggatgtcg agctcgatta cctgaagggc gactcgctgt
    29641 tgcactcgat cgaggacacc gtcaaccgca gcaacgactt ccgcatcgtc ctgacgccgg
    29701 gcaatgaccc gctatcgcac agcgatcatt tccacatcga agcctcggta gactacacgg
    29761 ccttccgcta gattccgggc atggccgacc cagtcgttga agtagcgacc acccacggaa
    29821 actttgtcat ccagctcgat ccggcgaagg cgccgaagtc ggtggagaac ttcctcgcgt
    29881 acgtcgacgc gaagcactac gacaacacga tcttccatcg cgtgatctcg acgttcatga
    29941 tccaaggcgg cggctacgac ccgcagatgg agaagaagcc gacgcgcgcg ccgatcacga
    30001 acgaggccga caacgggctc aagaacaagc gcggcacggt cgcgatggcg cgcacgagcg
    30061 atccgcattc ggcgaccgcg cagtggttcg tcaacgtcgt cgataacgcg ttcctcgatc
    30121 acaccgcgaa ggacggtggc ggctggggct acgcggtatt cggcaaggtg atcgagggca
    30181 tggaagtcgt cgacgagatc aagggtgtga agaccggcgc ggcgggcccg ttctcgaagg
    30241 acgcacccga ggacctcgtc atcatcaagc acgttcgccg ccgctagcga taagctaggg
    30301 catgcagtcg aggtggcttg cgctcgcggt tgtggtcgcc gcgtgcagtg atcctccgcc
    30361 cgtcgatgtc gagggtggcc ggctcaccgc aaagatctat cccgagccgc cgcgcatcgt
    30421 gctgttcgtc gacggcaacg aggtgtggtc gacgaccggc gacgcaggtg cgccgtatgg
    30481 ctttggcgcc gtcggcaatc gcgcagtcga gatcgagatg cagtttggct cgttccggtt
    30541 caaggagaac aagagccagg agtcgtggca ggggatcagc gagctgtctg cgatcacgct
    30601 tgcggacacc ggcgcgacat tcgacgtgcg aggcagcgat cgcaagctcg ggaccggcag
    30661 gctgacactc gactcgtcgt cgctgacagc gcgcatcgag ctcaccgtcg atcatgcgga
    30721 tcgattctcg atcagcggcg agtgtgcaac ggacgagcac ctcgtcggcc tcggtgggca
    30781 gtcgttcgac gtcgaccacc aaggcgagac cgtgccgctg tgggtgcagg aagacggcat
    30841 cggcaagttc ccggatcgcg acgaccagta tctcggcgtg tggtttctga cggggcgcaa
    30901 gcactcgacg cacacgccga tgccgatggt gctgtcatcg gctggctacg cgatcacgct
    30961 cgatacgaac gcgcgcgcga ccttcgacct cgggggcgag cgtcctggga cgtgccggtt
    31021 cgagacgtgg gagccgcatc tcgatctgcg cgtcttcctc ggcgagggcg gcgagccgcg
    31081 cgatgcgttc tcgaagatga tcaactgggt cggcaaaccg gcaacgccgc cggagctcat
    31141 ctatgcgccg tggatcgatg cgctgttcgg gtcggcgaac gtgcgacgcg tagcgcagaa
    31201 gcttcgcgat aatggaattt cgtcgtcggt catctggagc gaggactggc gtggcggcaa
    31261 ctatggcgcg accggctatg cgctcgagga agactggaag tcagatgcca cgctgtatcc
    31321 agacttctcg gcgctcgcgt cggacctgca cggcatgggc ttcgcgtttc acacgtacaa
    31381 caacacgttc atcgattcga ccgcggacat tcacaacgag gccgtcgaca acggctacgc
    31441 gatcaagaac ggcaacggcg atgcgtactc gttcaccggc gtgaagttca acccgtcgac
    31501 gatgctcgat ctctcgaacc ccgccgcggt cgcgtggggc aagcgcgtga tgaccgacgg
    31561 gctcgatgca ggcgccgacg ggtggatggc cgacttcgcc gagtggctgc cgcacgatgc
    31621 ggtgctcgca tccggcgaag atccgatgct cgtgcacaac cgctacacgg tcgattgggc
    31681 gcgcatgaac aaggagatgt tcgcgtcgcg gccgaacggc atctacttca tgcgctcggc
    31741 gtggttgcac agccaacccg agacgatggt gatgtgggca ggcgatcagc agacggattg
    31801 gagcgaaggc gacgggtttc cgtcggtgat tccgatcggg cttggcctcg gactcacggg
    31861 ctttccgtat ttcggcagcg atgtcggcgg ctacatgagc caaggcacga ttccgacgag
    31921 cgaggagctg ttctatcggt ggacgacgct cggcgcgctc tcgccggtca tgcgcacgca
    31981 tcatggacgc agcgtcatgg agaacgtgca gtgggagcac gacgcgggaa cgatcgcgca
    32041 cttccgtcgg tggtcgcgtt ttcacatgca gctcgcgccg tacttcaccg gctccgcgcg
    32101 cgggtacgaa cgagacggca tgccgctgtt ccgcttgatc gcgctcgatt atcccgacga
    32161 ggactgggcg tggagcgcgg tcgacgagta tctgctcggg gatcgaattc tcgtcgcgcc
    32221 ggtgctcgcc gagggcacga cgtcgcgcac gctgaagctg ccggcaggca cgtggtatcc
    32281 gctcatgagc gggccgcccg tcgcaggtgg agacgtcacg gcgcaggcga gcaagacgga
    32341 gattccggca tatgtgccgg ccggctcgat cctcgtgctc tatcccgatg gcatcgacac
    32401 cgtgctggcg tcacctgcgc tcgggaccgc gaagcagccg acgggcgcgc gtgaggtgtg
    32461 gctgtactcc gggacagcag cgaaccccgc gttcggtaca tggtccgatg cggatgggcc
    32521 aggcggcgcg gactggacct ggagtggccg gccatccggt gcgctgccca gctcggcgac
    32581 gttcaacggc agcccggtct cgctgacgat gggcgcggag tgggcgtcgg tgaccgtgag
    32641 cggaaacggg accttggcgt acgcgggtgg cggcacgctg acgatccaac gcggcgtctc
    32701 cgcgacgacg accgtctatc tgcgctgact ttttagggac cattttcggc tcccgggcgt
    32761 ctgttggatg gatgcggtcc gtcctcctgc tcgtcctgct cgcggcatgt gcgactcgcg
    32821 gcgccacgcc ggcaccgcac caccacgtcg atgcgcctcg tcaagcgatg ccgaaggcgc
    32881 tgcgcgctgg cgatctcctc ggtgtgcccg gcgagagtgt cgagtatcgc gtcgcgatgc
    32941 gcggcttcac gctcggtagc gtcgtcgtcg ccgttggtca gatcggcgag gtggaagggc
    33001 atcgcgcggt cgtcgtgcgc tcacgtggat ccggcagtgg tgcgttcgcg ctgttcagcg
    33061 agatcagctg ggagctgaag accgtgctcg cgctcgacct cggcaaggcg ctctccgaag
    33121 aagagacgat cgacgtcgag cttcccgtcg gcaagaagga gcacgagcac gagacgcgcg
    33181 atctgacggc cgatccgacg cacaacctgc acagcgccgc cggtgcgctc cgcgcgtggc
    33241 gctcgaagct cggtgacacc gcaacgttcg acgtgcgcgt cgtcgatgcg tacttaacgg
    33301 tagagctcac ggacgcggcg cgcgagaagc acggcgacat gccggctgtt cgctacgacg
    33361 gcgtcgcaaa ggcgacgggc acgtacaagc tctcgatctg ggtgtccgac gatgaaacgc
    33421 gcgtgccact gcgattcacg tcgcagacga agtggggcga ggtcgtcgtc gatctcgtga
    33481 gctacgacgt cgaccgcgcg ttgctcgtca accgttgacg cggcggatcg gcgtcgtcat
    33541 ctgcgcgtgg ctcgtgccga gcaacgccga gcgaagcagg ctgcgcgaca gcggcggctg
    33601 caggacgcgc tcgatgcgaa gcgagctgcg cagctggagc gacgcatcgc cgaccgcgaa
    33661 cacggtgccg aaccagccct gctcgcgaat cgcgtggagg tgaaggagct cgcccggcga
    33721 catcgcgccg atgttggcga acacgatctg cgggcgcggc ggcggatcct cgacgagcgc
    33781 cgagacgagc tggtcgacgc tgcgcgcggt ctgcacggtg atgtgcagaa gggccagctc
    33841 cgcttcgacc cacgtgtggt ggaagacgtc cgggatgtag caaagcgccc gcgtgagctc
    33901 gccgatcggg acctcgatgg gagtaccgcg cgcgcgaatc cgagggtggc tgggcatacc
    33961 tagtgactcg gccactcccc gaggatgtaa agggcccact ttgcgctaag gtccccccgt
    34021 cgtgccagcc gaagctcccg agaccactgc cgaagcccct cctcaggccc cccagatcgg
    34081 cttcccgaag aagacgctct actggatcgc cggcgtcacg atcgtcatct gggcgttcgc
    34141 gatcagcacg gggaacacga tcttcatcgg catcgtcggc gttctgacgg cggtgctcgc
    34201 gttcgtgatg attcgcgcgt ttctgttcat ccggaagcag cgcggcgtcg tggacattct
    34261 gcaaggcgca cagggctctc ccgaggcgcg caaagaagcg ctcgccaagc tcgagcaggg
    34321 caaggacgca aactcgccga cgacgctctt cgctcgtgcg cagctgatgg ccgccgacga
    34381 tccgaagtcc gccctgcagc tgctcgacaa gacggacctc aaagcattcc cgccgggcat
    34441 gcaggacgac gtctcgctcc tgcgcacgca gctgttcctc ggcctcggcc gcaccgccga
    34501 cgcgcgcaag gccgccgata cgatcaacct cgacaatccc gagcgcaaag ccgcgcgccc
    34561 gctcgcggca tcgatcgtcg ctgaagcgtg ggcgcgcacc ggcaagccca aagaagcgct
    34621 caagctgctc gactcgatgg aagtcccggc aaaggatgcc gagcaaatcg gaatccaggc
    34681 aaaggtcgct cgcgtgttcg cgcgattcgc cgccaaccag cgcggcctcg cacgcaacga
    34741 gctgaactcg ctcgccgacg acgatgtgaa ctacctcggc cgcttcctcg atccgcgctt
    34801 caaggtgcac cccgagctgc agaagctcgc gcgccaagtc ctcgagaagc atcccgcggc
    34861 gcgcaaagtc atcaagcagc aagcgcagcg acgctgaaat gggcgcactc tcgcaagccg
    34921 cgttcgagga gctggtcaac gcgccgtgcc cgtcgtgcgg cgagcgcgcg ctcgagattc
    34981 acacgttcat cgatcgcagc ttcgtggtga tggtcggcga tccgaacgat gcgggtcgct
    35041 gggtccacga cggcgagaag ttcgtcgacg gcacgtatcg aatcgcgtgc accaagtgcg
    35101 cgcacgccgc gttctccagc gacatgtgcc cgcgctgcaa cgcgccgcat gcgctcgaga
    35161 ccgcgctctc gtcgacgtcg cgcgtgacga ttccgaagcg ctgcccgaaa tgcaacgagc
    35221 tcgagctcct cgcgatcgcg ctcattccag caaaggcgcc tgcgacgacg gcgccgaagc
    35281 ccaagcccac tgccgaattc ggcgagcccg gctaccacat cgtcgcgttc gcctgtcacg
    35341 cttgcgacaa cgcggtcgtc agcgaacgct gcccgttgtg cgatgctcca ggtccgcttc
    35401 gccctcgccc ctaagtcatg ctgccgctcg tctttctcct cgtcgcgatc gccgtctggt
    35461 ggtggatgtc caaccagccg gtcacggtcc cacgcgtcga ggaagtcacc gcacgcatcg
    35521 cgttcccgcg gccgcttcgc ctcgacagtc ccgaccacgc gatcgcccag ctcgatcgcc
    35581 ccgacgagat cgtcatcccg catcagtacg cgaacctcgt gatcagcttc ccactgtcga
    35641 agcccgtcac gctcgcgatc gaatcgccga tcccgtacgg cttcacgcgc gccgagctcg
    35701 tccgcgcgat ctgccacgag tacgacacga tctacgacat cgaagaagcg accgccgcga
    35761 cgaagccgct gcctcccgtt ctccgcgaca acctcggacg caaccgcacc gacggcctct
    35821 acggcatctg gggccacgat ctctcggagc tcgtctgcac cgcgatccac tggacgcgtg
    35881 ccgcggactc gcgcgtgacg atccgcccgc acgtcgaagt gcaagagaag ccaaagctcg
    35941 acgccgacgc gtcgcccgac ggcgacaagc ctgcgcccta agcgggcggc cgactacggg
    36001 cagcgtgtcg agatcgcgat cgtgttgccg tcgcgcgtcg cgacgacgag cgtcgtctcg
    36061 tcggctgtga cgtcgacatc gtgcaggttg gtgtcgttga acagcacgct gccggcggga
    36121 aaggccgacg tcgtcgagcc gcgcacggtc ctgtagatcg agccagacgt gccgacctcg
    36181 tagtagagcg tgagctcgtc gccggagatc gcggggaacc gaaacatcgt gccgaggctc
    36241 acgccgttcg ccgtgaaggc ggcagtgcgc gagccgcgag tcgcagaacg cagcgtttgg
    36301 ctcgtgacgt agtagaccgt caagccgtcg gccgagaggt cgagcgacgc cggcgtcacc
    36361 gtcgtgaggc cggcgagggt cgcgggattc ccccacgcat cagtcgccgc gctgcgcgtg
    36421 acttcatgta gctcagcacc ctgctcgttc gcgcctactc ggtacatcag gacaagtccg
    36481 tcatcgctga tcgatggatc ctggtcgatg ccgcttgggt tgtcgatgat cggcagcgtg
    36541 cctcgatcgg agaagatatc ggtcaccgcg tcgcggcttg cccagtggat ctcccagctg
    36601 gtcgcgctgt tctggacgct ccagaacagc tgcagcttgt cgccggtgat cgtcggctcg
    36661 cccgtgacaa ggttcgtcag ctcggtgagt ggagtcggcg tcgtccaggc gcccgcgcac
    36721 gccgcgctat caccggcacc atcgcgctga gcatcagcga cagcggcgtc atcgcgcggt
    36781 cgtgcgggcg tcgccagaca acctgcgagg acaatcgacg cgaaggcaat gcgagtcagt
    36841 gccattgcac ggtgaccatg gcggagtgcg aaccgaccga cggtgcaacg acaagtgcat
    36901 ctcgatcgcc gctgcgcttc acgtgcaggt acacgccgac tccgacggca acgcccgccg
    36961 cggcgaacat ggcgacgtcg atgttgcggc gcgtgcgaaa cgccgacacg ccatcgtcga
    37021 acgcctgtgc cgactgcgtc aggtctttgc gcgcgggctg cacggcgagt aggtcgtagg
    37081 tcagcgcgac gacacccagc gcagcgccac ctgcgatcac atagagaccg aggcggctcc
    37141 gtggctgcga ctccgcgacg acgtcgagcg gcggcggctg cttgctcggc tcggccgcga
    37201 gcgggctcag cgtcacgtcg tacgtggtcg tggcacccgc gacggcggtg aagtcttgcg
    37261 tgtacgggat gaatccctcg cggcgaccga cgatcacgtg gcgccctggc gcgacgacgc
    37321 gctgcacctt gccgggacat gcaagcagag gctgaccgtc gagcgtcacg cccgaacgtt
    37381 ccgtcgtgca cgtgatgtcg agctgcgcga gctgaccgag cagctccttg cgatacgcct
    37441 tgcccttctc gagcttctcg gcaccgagcg gtgcctcgcc gaacttcagc gcgcgctcga
    37501 gcatctcgaa cgcctcgagc ggctcgttca tctcgatcag gcagaccgcc atgttgtagc
    37561 gaatcgcggg atggtcccaa tggacgaccg cctcgcgata cagaacgagt gcctgcgagt
    37621 aacgcgattg cacgaactcg tcgttaccct tgcgatagat gtccagcgcg agcgtttgct
    37681 cggacacagg gacacctttt gcccaaggag tgtcctcggc caatgcggtc gcggaggtcc
    37741 ccacgaccat gcatacgatg agcagccctc tcacagctgc taagggtatc agtcgcgctt
    37801 gtacatcgtc atcacgcacg caccgccgag gccgaggttg tgctgtagcg cgatcttcgc
    37861 gccctcgact tggcgatttt ccgccgtgcc gcgcaggtgc cacacgagct ctgtgcattg
    37921 cgcgaggccg gtggcgccca gtgggtggcc tttggagagc aggccgccgg acgggttcgt
    37981 gacgtacttg ccgccgtacg tgttgttgcc gtcccagatg aacttctcgg cttcgccttc
    38041 tttgcagagg ccgatcgctt catacgtgag cagctcgttc gccgtaaagc agtcgtgcag
    38101 ctcgacgacg tcgacatctt cggggccgat gcccgtttgc tcgtagagct tcttcgcggc
    38161 gttcttcgcc atgtcgaagc cgaccatctt gatcatcgag tgctcttcga acgtcgacgg
    38221 atagtcggtc gtcatcactt ggccggccat gtagaccggg ttcgagatgc cgtgcttctt
    38281 ggcgaactcg tcggagcaga ggacggccgc ggccgcgccg cacgtcggcg ggcagcactg
    38341 gtagcgggtg agtgggtcga agacttccgg gctctgcatg atctgctcga gcgagtacgt
    38401 ctccttgaac agcgcgtacg gattcttcga cgcgtggagg cgagctttct cggcgatctt
    38461 gccgaacgtc tctttcttcg tgccgtactt ccagcggtac tcgcggccgg cgccgccgaa
    38521 catctgcgcg gcgggcggag cttggttgaa gccctgctcc tcgttcatca cgttgacgaa
    38581 cttgtcgagc gggttcgcgc gatcggtcca cttcgcttgc agcgcgcctg cttccatctg
    38641 ctcgaagccg acgacgatca cgcactcggc gttgccgccg gagaccgctt gcgcgccgag
    38701 catgagggca gagctgcccg tcgagcagtt gttgttgacg ttgaagacgg ggatgcccgt
    38761 gaggccgagc gtgtagatcg cgcgttgacc gcacgtcgag tcgccgtaca cgtagccagc
    38821 aaatgcttgc tgcacttccg tgaacgcgac cttcgcgtcg tcgagcgcag ccttgccggc
    38881 cttgctcgcc atcacgtgat actcctcgct cttacccggc ttctggaacg gaaccattcc
    38941 gacgccgatg acatttacct tgcggggcat tagatggctc ctttcaaaaa gccgaggcga
    39001 tgcgcgacgg tgacgtcacc gtcgaccttg agcttgccgt gttgatagag agacttgacg
    39061 ttgccgctcg cgagagccgg cagatctgaa tccgcgatcg tgagcgtggc cgccgtatcg
    39121 ccgccgagct cgaacgtctt cgtcgcgtcc gggccggtga cgttgaacgt gaccttcgcg
    39181 cgaacttctt gcgcgagggc cttgttctcg gacagccgct tctcgagcgc cgcgaagaac
    39241 ttcggcgcgt tcggatccga cgaggccttc gccgccgccg gagcagccgc cgccggagca
    39301 gcagccgcac caccgccgcc accacgacgc gccatcaccg tctcgatcgc cttgctcggg
    39361 tcgatcttga acagcgactg cagcttctgc gacgccatca cgttgccgct gatcttcagc
    39421 ttgcccgtcg tgaacagctt catcggatcg gctttgcctt gcgtcagatc gaggaagtcc
    39481 gactcggcga gctcgagcgt gcactcggca gcgccttcac ccgtcgacac cttgccggcc
    39541 ttgagatcga cgatgaagct cttcgagccg accttgaagc cgtagacgac gccgaccttc
    39601 tggacgacct cggggttctg cttgatgact tcgccgaata cttcgaatgc gtcgtcgacg
    39661 gtcggcgtgt atgccgcagg cacagaggca gcagccgctt gcgatgcacc acccgaacgt
    39721 ttcttcgttt ccgcgagcac catctccggc gtgatcttct tcaggaagcc cagcttctgc
    39781 gacgccatca cgtcgccact gatcttcagc ttgcccgtcg agaacagctt catcgcatcg
    39841 gcttggccgg tcgccatcgc catgaaatca gcgtcgctga tctcgagcgt gcaggccgcc
    39901 ttgccgaccg cggagtcgtg aacagcgccc ggcggcgtcg acagatcgat cgtccacgtc
    39961 gagtccgggc tgttcagctt gaacaggaac gtggtcttca ccttctcggc cgtcgcgggg
    40021 ttgcccgcaa cgaagccgcc gatcgcacgg aagatgtccg cgct
//
LOCUS       DATLBZ010000023         7377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5972109, whole genome shotgun sequence.
ACCESSION   DATLBZ010000023 DATLBZ010000000
VERSION     DATLBZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5972109"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VMZ53_01105"
     CDS             <1..433
                     /locus_tag="VMZ53_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27066.1"
                     /translation="IDAAVGTSDGPTVDAPLRAATCADPQPFPATGGVTGGTTAGRMN
                     SVSAMCSASVMNGADAVYRIGATTGAQLLVSVSGYTNVAAYVIGACTGTPATPICLGS
                     TAAMPGSPINVTASSTGQYFIVVDSANAGASGAYTLTVDVQ"
     gene            577..1863
                     /locus_tag="VMZ53_01110"
     CDS             577..1863
                     /locus_tag="VMZ53_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27067.1"
                     /translation="MRWALLLLAGCTLGGTGRPGDGDDMGTHVDDTACGAAMAMAPLA
                     PAGYDFHDALPTATRNTWDGVALPQPGDASYPGGRYRTLAADGAGKPHPGCSVAGLTY
                     TPASIPGFACAAREFAPASEDTTKPIVILVHGNSESPTGWMKFVHADPASLMFPADTT
                     ARDQLAEILPAHGFRTIAIDMRSDKIDDPKSPEGKDIGNTPKNADHGWTVPLLQELVK
                     RLAIAYPDRKLALVGFSLGATTVRDALRRLWVEKQDGKWDVNILSRVETVVVASGANH
                     GVVSFAKQCGLNLTMRGTVTCEMGQRNQYTETAFHRPLNGPPIDGEMQFGGWWETPCA
                     DGDYAFGKRGACGGNKVAYTTITMQDLANGTQQDEFVSEHASRLYPKECAVNELDGLN
                     DFDTSGYFYNGFFRNHYGSVRSDAGIAKVLAALERK"
     gene            1866..3062
                     /locus_tag="VMZ53_01115"
     CDS             1866..3062
                     /locus_tag="VMZ53_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27068.1"
                     /translation="MRLAFCVLLISSVAYADEAVTEDIEVEPAKPKRWLEPYGAIAGG
                     MRVESLHQPADAERGTQNPTVAVSRLGLRGGYGEHITFASEFEAALGGPQGYGASVWE
                     GQAALAVRDQFVRYARSGASIAAGRIADPASFDFASMHIGDLLYTDLYTRDPLLYSGA
                     DRGNGIIGTYAINDHITTGLTFHSTNPTGITGTLIIGGKLQPFDRPFYLASAAVGNNQ
                     NNLPDQNLHIYFGSPSVRVHYDNFEAQSEVQLYTLDTQQAIADDQTIRGYNLRLGGRA
                     WTQTAIGKLTGFANVSRNRNEILDPVDSKYRLPDLFRSYTVSLGADVDYQRKNGVGFE
                     YAMADTREPDQHTRLHFINLATTYWIEDVLAIAVRGSVYAQQISGEMMTTGSRSLFVT
                     ARLVLD"
     gene            complement(3059..5563)
                     /locus_tag="VMZ53_01120"
     CDS             complement(3059..5563)
                     /locus_tag="VMZ53_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HUS27069.1"
                     /translation="MRCWLVALLLLGGCLESDLVRCGDKLCPVGAFCTPEGCVLQDQI
                     DVCRNKADGDECQVLNLPAGFCRNGTCVSAECGNALMEPGEACDDGNHDEGDGCSVDC
                     RSLESCGNDIIDLVKSEQCDSGVPQMSGDGCTSTCQAEFDIWRDVTPQQLIGRWGAKM
                     AFDPAHNEIVLFGGNNNGALGDTWILRGGAWEERSPASSPEARYGHAMAYLPGVGVVL
                     FGGTTGTVYPSGTWVWNGSQWTKLNIGAGAASPHVRTGAAMALNPQTGHLMLFGGGFG
                     GTVYADTWDFDGTTWTKLSAPDVAGMTWVSMFVDPNAAASNSGAVWIIGSDGSATGNK
                     TLYWNVSQWVAHAPSGAPAYVDPAIAIDHNRVVLFGGRDPNTNAPTNTTFFWDSGNRN
                     WVQQTVVGTPAARWKIAAANDGANMVMFGGLSATDSPLANTYQLTTVTGPPVTYRYTS
                     IPTTPRPISRYGATSVYDARTGEVFIFGGFTLDNSEEGDLWRWTFPLWRTGASGPDPR
                     MNASMTYDEAREETVLFGGVDDCGTGTGSGSGGLCGSGSGGGSGTGARVYFDDTSTLK
                     SNMWETRTLTLKPPPRADAAIAYDPNAQVVILFGGQNATTSFDDTWKWDGTAWTKLLP
                     ATSPPPMWGGRLVYDRARSLFVLYAGTGALATDLGRVWTFDGTTWHDATPAATLAQPP
                     ARRRCVFVYNPRRQRSLLFGGVLPNGQILNDTWEWDGVAWEQRHPVQAPSPRYDAAAT
                     FDASRGHVLVFGGAGQVNALQDDVWVYNFVSNSETVERCDGMDADGDQLVDCADPDCY
                     WRCDPYCPPGSIMCAPDRPRCGDGKPSPLETGFCAP"
     gene            5589..6530
                     /locus_tag="VMZ53_01125"
     CDS             5589..6530
                     /locus_tag="VMZ53_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27070.1"
                     /translation="MANALENALQKALSVRLNLQISLSQLPMIEEWMDIHLDRWLEHN
                     PTPPDMPDGEGVSYLAMLGSDLRRVWWGAWGDPRGFVPRMADYFKLCNIAKSDAQLLD
                     GIGEKFEPRLVGSWIGVWGGKVTTGWHFWDPQPWSKVEAMFGTHEAKFKLKKFVETHG
                     IDRIERFTQSIGDSPFSEIEIAMNGDTVDAQVDALSQAFVDFTGAPLSGPVLERMRGA
                     SVPQFGLSVRIRGGQIVRIGGLAPGISLDAITKLCTDAKIGVDEKLPKLVGALGDGIT
                     RVEYGRAGERAGVDVYLEPGEPASKPGAVKPASSDAN"
     gene            6530..7051
                     /locus_tag="VMZ53_01130"
     CDS             6530..7051
                     /locus_tag="VMZ53_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HUS27071.1"
                     /translation="MPRVDYLLASDDALIGQCEVDRYRASGPGGQHRNKTESAVRLRH
                     KLTGIAAIGEDSRSQAENKMHAVRRLRSALALAVREPVQLDGYVPSKRLAAFVAAGTA
                     PLGAKTKLTGEYWAAIGELLDLLVAGNLEIGTTAQRLGISTGALSKLLLHDDTVGRAV
                     NDLRRAKAMRPLR"
     gene            7061..>7377
                     /locus_tag="VMZ53_01135"
     CDS             7061..>7377
                     /locus_tag="VMZ53_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27072.1"
                     /translation="MRWALILVVLAACRFGFEARTGELDDADTDVTTGDIGPVPTCAG
                     HDEDGDHFPDACDSCPTVPNGAQVDGDGDGVGDACDPRPATGGDYIQLFDAHTSSTSG
                     TYYV"
BASE COUNT         1280 a         2422 c         2415 g         1260 t
ORIGIN      
        1 tatcgatgcc gcggtcggca ccagcgatgg gcccacggtc gatgccccgc tacgcgcggc
       61 gacgtgcgcc gatccgcagc catttccggc gaccggcggc gtgacgggcg gaacgaccgc
      121 gggccgcatg aacagcgtgt ctgccatgtg cagcgcgtcg gtcatgaacg gcgcggatgc
      181 cgtctatcga atcggtgcca ccacgggcgc gcagctgctc gtcagcgtga gcggctacac
      241 gaatgtcgcg gcctatgtga tcggcgcgtg caccggcaca cccgcgacgc cgatctgcct
      301 tggaagtacg gccgcgatgc caggcagtcc gatcaacgtg accgctagct cgacgggcca
      361 gtacttcatc gtcgtcgatt ccgcgaatgc gggcgcgagt ggcgcgtaca cgctaaccgt
      421 cgacgtgcag tagctgttgg gcaccgccaa caggggtcag gcacaagttc acaagttgcc
      481 aacaccacaa cttgtgaact tgtgcctgac cccgtccaca acttgtgaac ttgtgcctga
      541 ccccagtccg gaacgctgct tgctcctgcg cgcggcatgc gatgggcact tcttcttctg
      601 gcgggctgca cgctcggcgg caccgggcgt ccgggcgacg gcgacgatat gggcactcac
      661 gtcgacgaca ccgcgtgcgg cgcagcgatg gcgatggcac cactcgcccc cgcgggctac
      721 gactttcacg acgcgctgcc tacggcgacg cgcaacacgt gggatggcgt tgcgctgccg
      781 caaccgggcg atgcgagcta tccgggcggc cgctaccgca cgctcgctgc cgatggcgcg
      841 gggaagccgc atccgggttg cagcgtcgcc gggctgacgt atacgcccgc ttcgattccc
      901 ggctttgcgt gcgcggcccg tgaattcgca ccggccagcg aggacaccac gaagccgatc
      961 gtcatcctcg tgcacggcaa ctcggagtcg ccgacgggat ggatgaagtt cgtgcatgcg
     1021 gatcctgcgt cgctgatgtt tcccgccgat acgaccgcgc gcgatcagct cgccgagatt
     1081 ttgccggcgc acggatttcg cacgatcgcg atcgatatgc gctcggacaa gatcgacgac
     1141 ccgaagagcc ccgagggcaa ggacatcggc aacacgccga agaatgccga ccacggttgg
     1201 accgtgccgc tgttgcaaga gctcgtgaag cggctcgcga tcgcgtatcc ggatcgcaag
     1261 ctcgcgctcg tcggcttctc gctcggcgcg acgacggtgc gcgatgcgct gcggcgcttg
     1321 tgggtcgaga agcaagacgg caagtgggac gtcaacatct tgtcgcgcgt cgagaccgtc
     1381 gtcgtcgcgt cgggcgcgaa tcacggcgtc gtcagctttg cgaagcagtg cggcctcaac
     1441 ctgacgatgc gcggcaccgt cacgtgcgag atgggccagc gcaaccagta cacggagacc
     1501 gcgtttcatc ggccgctcaa cggaccgccg atcgacggcg agatgcagtt cggcggttgg
     1561 tgggagacgc cgtgtgccga cggagactac gcgttcggca agcgcggcgc gtgcggcggc
     1621 aacaaggtcg cctacacgac gatcacgatg caggacctcg cgaatggcac gcagcaagac
     1681 gagttcgtca gcgagcacgc gtcgcggctc tatccgaagg agtgcgcggt gaacgagctc
     1741 gacggcctca acgacttcga tacgagcggc tacttctaca acggcttctt tcgcaatcac
     1801 tacggcagcg ttcgcagcga cgcgggcatc gcgaaggtgc tcgccgcgct cgagaggaaa
     1861 tgatcatgcg tctcgcgttt tgtgtcttgt tgatctcgtc tgtcgcgtac gccgatgaag
     1921 cggtaaccga ggacatcgag gtcgagccgg cgaagccgaa gcggtggctc gagccatatg
     1981 gcgccatcgc gggcggcatg cgcgtggaga gcctgcatca acccgccgat gcggagcgcg
     2041 gcacgcaaaa tcccaccgtc gcggtctcgc gcctcggcct gcgcggcggc tacggagagc
     2101 acatcacgtt cgctagtgag ttcgaggccg cactcggcgg tccgcaaggc tatggcgcat
     2161 cggtgtggga agggcaggcg gccctcgcgg tgcgcgatca gtttgtccgc tacgcgcgga
     2221 gcggcgcgtc gatcgctgcg ggacgcatcg ccgatcctgc gagcttcgac ttcgcatcga
     2281 tgcacatcgg cgatctgctc tacaccgatc tctatacgcg cgacccgctt ctctactcgg
     2341 gcgccgatcg cggcaacggc atcatcggca cgtacgcgat caacgatcac atcacgacgg
     2401 gcctgacgtt ccactcgacg aatccgaccg gcatcaccgg cacgctcatc atcggcggca
     2461 agctgcagcc gttcgaccgg cccttctact tggcgtcggc ggccgttggt aacaaccaga
     2521 acaacctgcc ggatcaaaac cttcacatct acttcggctc tccgtcggtg cgcgtgcact
     2581 acgacaactt cgaagcacag agcgaggtgc agctctacac gctcgacacg cagcaggcga
     2641 tcgcggacga ccaaacgatc cgcggctaca accttcgcct cggcgggcgc gcgtggaccc
     2701 agaccgcgat cggcaagctc acggggttcg cgaacgtgtc gcgcaatcgc aacgagatcc
     2761 tcgaccccgt cgactcgaag tatcgcctcc ccgatctgtt tcgttcgtac accgtgtcgc
     2821 tcggcgccga cgtcgactat cagcgcaaga acggcgtcgg cttcgagtat gcgatggccg
     2881 acacgcgcga gcccgaccaa catacgcgcc tccacttcat caacctcgcg acgacgtact
     2941 ggatcgaaga cgtcctcgcg atcgcggtgc gcggctcggt gtacgcgcag cagatcagcg
     3001 gcgagatgat gacgaccggc agccgctcac tgttcgtcac cgcgcggctc gtcctcgatt
     3061 agggagcgca gaagccggtc tcgagcggcg acggtttgcc atcaccacag cgcgggcgat
     3121 cgggtgcgca catgatcgag ccaggtgggc agtacggatc gcagcgccag tagcagtcgg
     3181 ggtccgcgca atcgacgagc tggtcgccgt cggcgtccat tccatcgcag cgttcgacgg
     3241 tctcgctatt ggagacgaag ttgtagaccc agacgtcgtc ttgcagtgca ttcacctggc
     3301 cggcgccacc gaacacgagc acgtgaccgc ggctcgcatc gaacgttgcc gctgcgtcgt
     3361 agcgcgggct cggtgcctga accggatggc gctgctccca cgcgacgccg tcccactccc
     3421 acgtgtcatt gaggatctgc ccgttcggca gcacaccgcc gaacagcaag ctgcgctggc
     3481 gacgcgggtt gtagacgaac acgcagcgac ggcgcgctgg cggctgtgcg agcgtcgctg
     3541 cgggtgtcgc gtcgtgccac gtcgttccgt cgaacgtcca cacgcgtccg aggtccgtag
     3601 cgagcgcgcc ggttcctgcg tagagcacga acaaggagcg agcgcggtcg tatacgaggc
     3661 ggcctcccca catgggcggc ggtgatgtcg ccggcaacag cttcgtccac gccgttccgt
     3721 cccacttcca ggtgtcgtcg aacgacgtcg tagcgttctg acctccgaac aggatcacga
     3781 cctgcgcgtt cgggtcgtac gcgatggcgg catccgcgcg tggcggtggc ttgagcgtga
     3841 gcgttcttgt ctcccacatg ttgctcttca gtgtcgacgt gtcgtcgaag tagacgcgcg
     3901 cacccgttcc actgccgcca ccgctaccgc tgccgcagag tccgccgctg ccgctgcccg
     3961 tgccggtgcc gcagtcatcg accccgccga acagcaccgt ctcctcgcgt gcttcgtcat
     4021 acgtcatcga tgcgttcata cgagggtccg ggccggacgc cccggtacgc catagcggga
     4081 acgtccatcg ccacaggtca ccttcctcgg agttgtcgag cgtgaagccg ccgaagatga
     4141 agacctcgcc ggtacgtgcg tcgtatacgc tcgtcgcgcc gtagcgggag atcggccgtg
     4201 gcgtcgttgg gatcgacgtg tagcgatacg tgaccggtgg gccagtgact gtcgtcagct
     4261 ggtacgtgtt ggcgagcggg gaatcggtcg ccgataggcc gccgaacatc accatgttcg
     4321 cgccatcgtt tgcggccgcg attttccagc gcgccgctgg cgtaccgacg accgtttgct
     4381 gcacccagtt ccgattgccg ctgtcccaga agaacgtcgt gttcgtcggc gcgttggtgt
     4441 tcggatcccg accgccgaac agcacgacgc gattgtgatc gattgcgatc gccggatcga
     4501 cgtacgcggg cgcgcccgac ggcgcgtggg ccacccactg cgagacgttc cagtacagcg
     4561 tcttgttgcc cgtcgcactt ccatcgctgc cgatgatcca gacggcgccg gaattggaag
     4621 ccgccgcgtt cggatccaca aacatcgaga cccacgtcat gccggcgaca tcgggcgctg
     4681 aaagcttcgt ccacgtcgtt ccgtcgaagt cccaggtgtc ggcgtaaacc gttccgccga
     4741 agccaccgcc gaacaacatc aggtggccgg tctgcggatt gagcgccatc gctgcgccgg
     4801 tgcgaacgtg cggtgaagca gcgcccgcgc cgatgttcag cttcgtccac tggctgccat
     4861 tccagaccca cgtgccgctc gggtaaaccg ttccggtcgt gccgccgaac aggacgacgc
     4921 cgacgccggg cagatacgcc atcgcatgcc cgtagcgagc ctctggagag ctcgccggcg
     4981 atcgctcttc ccatgcgccg ccgcggagga tccacgtgtc gccgagtgca ccattgttgt
     5041 tgccaccgaa cagcacgatc tcgttgtgcg cggggtcgaa cgccatcttc gcgccccacc
     5101 gaccgatcag ttgctgcggc gtgacatcgc gccagatatc gaactccgcc tgacacgtcg
     5161 acgtgcagcc gtctccggac atctgcggaa cgccgctgtc gcattgctcg ctcttgacga
     5221 gatcgatgat gtcattgccg cacgactcga gcgaccggca gtccacggag cagccatcgc
     5281 cctcgtcgtg gttgccgtca tcgcacgctt cgcccggctc catgagcgcg ttgccgcact
     5341 cggccgaaac acacgtgccg ttgcgacaga agccggccgg taggttgagg acttggcatt
     5401 cgtcgccatc cgctttgttc cggcagacgt cgatctgatc ctggagcacg cagccctcgg
     5461 gagtgcagaa cgcgcccacc gggcacagct tgtcgccgca tcggacgagg tcactctcga
     5521 ggcagccgcc gaggaggagc agcgccacaa gccaacaccg catgcgtttc acgatacact
     5581 ggcgtcgcat ggcgaatgcg ctcgagaacg cattacagaa ggcgctgtcc gtcaggctga
     5641 acttgcagat cagcttgtcg cagctgccga tgatcgaaga gtggatggac atccatctcg
     5701 atcgctggct cgagcacaat ccgacaccgc cggacatgcc cgatggcgaa ggcgtgtcct
     5761 atctcgcaat gctcggcagc gatctgcgcc gcgtgtggtg gggcgcgtgg ggcgacccgc
     5821 gtgggttcgt gccgcgcatg gcggactact tcaagctctg caacatcgcg aagagcgacg
     5881 cgcagctcct cgacggaatc ggcgagaaat tcgagccgcg gctcgtcggc tcgtggatcg
     5941 gcgtgtgggg tggcaaggtc acgaccggct ggcacttctg ggatccgcag ccgtggtcga
     6001 aggtcgaagc gatgttcggc acgcacgagg cgaagttcaa gctcaagaag ttcgtcgaga
     6061 cgcacggcat cgatcgcatc gagcgattca cgcagtcgat cggcgattcg ccgttctccg
     6121 agatcgagat cgcgatgaac ggtgacaccg tcgacgcgca agtcgacgcc ctgtcgcaag
     6181 cgtttgtcga tttcacaggt gcgccgctgt ctggccctgt gctcgagcga atgcgtggag
     6241 ccagcgttcc gcagttcggt ctctccgtgc gaattcgcgg tggccagatc gttcgcatcg
     6301 gtggtctcgc gccgggcatc tcgctcgatg cgatcacgaa gctgtgcacc gacgcgaaga
     6361 tcggcgtcga cgagaagctg ccaaagctcg tcggtgcgct cggcgacggc atcacgcgcg
     6421 tggagtacgg gcgtgcgggc gagcgtgcag gtgtcgacgt gtatctcgag ccgggcgaac
     6481 ccgcgtcgaa gcccggtgcg gtgaagcctg cgtcgagcga cgcgaactag tgccgcgcgt
     6541 cgattacttg ctcgcgagcg atgatgcatt gatcgggcaa tgcgaggtcg atcgctatcg
     6601 cgcgtcgggg ccgggcggtc agcatcgcaa caagacggag agcgcggtgc ggttgcggca
     6661 caagctcacc ggcattgccg cgatcggtga agacagccgc tcgcaagccg aaaacaagat
     6721 gcacgcggtt cgccggctgc ggtcggcgct cgcgctcgcg gttcgcgagc cggtgcagct
     6781 cgacggctat gtgccttcga agaggctcgc cgcattcgtc gcggcaggca cggcgccgct
     6841 cggcgcgaag acgaagctga ccggcgagta ctgggcggcg atcggcgagc tgctcgattt
     6901 gctcgtcgcg ggcaacctcg agattggcac cacggcgcag cgcctcggaa tctcgacggg
     6961 ggcgctctcc aagctccttc ttcacgacga caccgtcggg cgagcggtca acgatctacg
     7021 tcgcgccaaa gcaatgcgcc cgttgcgata agctaccagt gtgaggtggg ccttgatcct
     7081 cgtcgtgctg gctgcgtgtc gcttcggctt cgaggcgcgc accggcgagc tcgacgacgc
     7141 ggacaccgat gtcacgacgg gtgacatcgg gccagtcccc acttgcgcgg gtcacgacga
     7201 ggacggcgat cattttcccg atgcgtgcga cagctgcccg acggttccga atggcgcgca
     7261 agtcgatggc gacggagacg gcgttggtga cgcgtgcgat ccgcggccgg cgacgggcgg
     7321 cgactacatc cagctcttcg acgcgcacac gagcagcacg tcgggcacgt actacgt
//
LOCUS       DATLBZ010000024        20833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9698472, whole genome shotgun sequence.
ACCESSION   DATLBZ010000024 DATLBZ010000000
VERSION     DATLBZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20833
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9698472"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VMZ53_01140"
     CDS             <1..870
                     /locus_tag="VMZ53_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HUS27073.1"
                     /translation="GRAIPGVEIVGDDHYRRVVHLGGHVGEIRVEPADRGTARGKSGQ
                     RETRITRGERGQSPKARAALLLTVSPALLPVLMPLVARVRRMFDLDAHPDRIASVLGR
                     DRALARHLAKRPGLRVPGAIDPFEASVRALLGQQVSVAAATTLAGRFATQLGVAYAGG
                     EGLAYRFPTPAEVVAAGAERIAKLGMPGKRGLAIHTFAKAIVDGTIRLDVATDLDAFV
                     DKLVALPGIGPWTAHYLAMRALHLPDAFPAADLGVQKALGRAGAKAAEKRAEAWRPYR
                     SYAVLHLWMGLGD"
     gene            873..1376
                     /locus_tag="VMZ53_01145"
     CDS             873..1376
                     /locus_tag="VMZ53_01145"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HUS27074.1"
                     /translation="MMLSMITMASPLGTLRLYADGDELTAVSLPCDAAAVANECSTPV
                     LDLARAQLTEYFAGTRRAFELPLAPRGTGFQQIVWRALTTIRYGETWSYGQLARVIGR
                     PAASRAVGAANGRNPLAIIVPCHRVIGANGTLTGYAGGLPAKKWLLQHEVAHGGHAAI
                     GTLFASC"
     gene            1386..2189
                     /locus_tag="VMZ53_01150"
     CDS             1386..2189
                     /locus_tag="VMZ53_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27075.1"
                     /translation="MNWFFLGGLAGLAGLLASRGLFRRANQSSAARASGLEPLGDLTH
                     IPSSLQKTALWALADGGFERRVVGGILARDGGDVTVTAFDLETLRERRGEWAWLPIEP
                     PFRIAPVVSIVVCEVERAFPHILLKRHGHGDQMADDTVLERVGSVSKLVRDGLGMARS
                     YAAELPAPLPATPASTTLPEGWRAYTQAPDLLETLLPAGLAAALERGGRRDLVVELLD
                     RLVVVYPAARDVVGADQFADLTTTALTIVDGVLAASPPVTPRGIDVTTS"
     gene            2243..3070
                     /locus_tag="VMZ53_01155"
     CDS             2243..3070
                     /locus_tag="VMZ53_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27076.1"
                     /translation="MRAGAILAVACAACSSGNGPDMAAPDASSSIDAVATTDALLSSD
                     SDGDGLADDLEDQLARDYLPFVSLDPGDGCTLSGFVARVRKHPADPTKILIVYSHLFQ
                     RDCGLGGHIGDNEAFGIAIDPAVPAPGGIQAIRTASHQNTPCQRTTECTTCSNDSRAR
                     CDFAIDAGAQWPVLYASKDKHGQYATKSGCPLIGTCFDQCTLNSARQVAPIINVGEPA
                     HHMVTNLTAQGFITPANGWTEAELMNVDPWNAMVDFGGAGNIAGDLEDSTFEPAPCQ"
     gene            complement(3205..4941)
                     /locus_tag="VMZ53_01160"
     CDS             complement(3205..4941)
                     /locus_tag="VMZ53_01160"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HUS27077.1"
                     /translation="MRAAFALLVLFVISACGDNIKPGVTPDAAPGVDVVQATCGDGMI
                     TGDEQCETGACCQNCKLAPFNTQCRQAANACDLAEVCDGLTNTCPSDLRAPDGTSCGT
                     NQFCSNGTCGTCNTATDADFDGSNQCVDCNDNNGLVKPQAAEHACEGLDDDCDGKIDE
                     DYDLDQDGYSVCSDDPLVKDCNDAAAAVHPGATELCGTAGTGNGVDENCNGYIDETCQ
                     PCDTTDNDGDGKSECQGDCDDAQPTVGPGKPEACDGFDTDCNKFTTQNCDVSGQCNWA
                     ANDVCKDDLECGCILDGTGVCTGNYRCASFCEGSFTGPIGAGCTSTQTCEYRWTESDN
                     QHACSETTATLGTKLGGATCNADNECRSGSCAILQVGQTQKYCIDFCDHDSTGGGGCA
                     TGTVCEILQQNTLADVFQYASCGLANGTQGQGQSCTGGCKWGTASCVSGTCSAPCGDN
                     SQCPSGTHHCSIMGNNVVTGTWTTGVPTDVTGKRSIEVVPVCRPNAGAGLHNRRGGAA
                     CTQNSDCTSEFCDANIHVCVELCTQDATCPVGLTCDPIYLQTSPATLTPSVTWGRACV
                     NASFGDLATRMN"
     gene            complement(4986..6461)
                     /locus_tag="VMZ53_01165"
     CDS             complement(4986..6461)
                     /locus_tag="VMZ53_01165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HUS27078.1"
                     /translation="MRLREFQWDDTPLGSPTTWPVSLRTAASICFASKQPMAIVWGIE
                     HTLLYNEACAKLIGLIDHTSAIARSGRSLQPWRSLVDAVDVVLAGQDPAAAEELAHDI
                     LNVTFTLTPIYSEGVRAEGVLVELHDGARVDVEQRAKDDFLSLFGHELRNPLSAITAS
                     LQVLVRQSPSVEVMVIDRSIRHLTRLVDDLLDASRLRRGKLELRRTTLEIAQVIDLSL
                     ERLSRTFEETKTQVFVRVPRSGLGVDCDPERMAQVFTNVLHNAAKYSDPGSVVNVEAE
                     RSGESVRIRIRDSGPGIESHALEQIFEPFNGARPTNAGLGLGLGISRKLVELHSGKLA
                     IRSDGGGSGTEVVIELPHTPAPDGALIPPINLERRKRVLLVEDNHDTAVALQRALETL
                     GYQVALAHNGPVALTVARTFQPDVALLDINLPVMDGWELSKRLRELKVPSRELHFVAV
                     TARDQEQDKQRSADAGFADHLVKPIDLNKLERVVENLPDPA"
     gene            6564..7040
                     /locus_tag="VMZ53_01170"
     CDS             6564..7040
                     /locus_tag="VMZ53_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HUS27079.1"
                     /translation="MLVIIHSGQTGVERGAHRAALASGLSIAGFMPLDRRDELGPIPP
                     DVAGSLTPCFDRGPRPAVRANISLASGVLLVVPNAAMPEKFTAMSAVLQGIRSAHCPS
                     LIADSAVNLDDVAKWATTLPETSGSTRIHVTGPRSTRWPEGESLARRLVAAIAMSQ"
     gene            7099..9525
                     /locus_tag="VMZ53_01175"
     CDS             7099..9525
                     /locus_tag="VMZ53_01175"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HUS27080.1"
                     /translation="MVRPLLALVLVLASASAATAASELPGKAGMYPQQGAPLAMLDSK
                     VVVRVHGPIAEAVVTQTFRNDTDRVTEATYIFPLPLDAAVTAMEIESGNRTIRASIET
                     RDQAQQRYERAVAEGVGASLLEQERPDIFTQTVSAIPARGKVTVTLRFDTVARYSAGT
                     WELALPLVIAPRYVPGTASGRPTTGSGRAPDTDRAPDASRVTPSGSPGAGGSTEVTLE
                     LAEVDDVTSPTHELVKQKGSYQLVDPKSDHDAIIRWRAKAPTAAWVEADDGGGYAAIV
                     VEAKPAAKPATAVKMLLVLDHAATTRGDADAVKKPLVRALLQTLTPKDRVRVAGSDSL
                     DTRAPADAWKALDEAWAKPHGAFDLAKVLARSDDPIVLVTDGLVADDRAVLAAAAKVG
                     APVHVIGVGPAPNRSLLAAIATTTGGTVRYAVVGDDAQAIARDVLADIATQPEPLTIN
                     WGTLAASDVVPATLPRLGANQAMLVLARVKKVQSANARVRGDVFGFTAVTSSKTPDGA
                     TTTHGALARRWAKLKLDDLVAAGNAKSITEHALRFGLVSPYTAMVAIGDEVVVQGGVK
                     HSVAVPVSVPAGMQWQHVKREITVDTTKTTQDFSSMKLDEDEKPAKKKPRRESEDVVG
                     GEASGAGSTKDFGDEESDGAPPENAPVSPSSVAMGMDYEEEADDAESIEVRGYRSRTR
                     YSLGLGGGLSIVNGTASPAFELSARVERLGRLRLGGEASLWLVDGLHGQGHFFATIGK
                     ALLPRFDLRGGPGLTLTGDGVGPAVNVTLRGRVSRNAAAYLRYDGALLIRSGAYDGQN
                     TGSFGIEASF"
     gene            9545..11698
                     /locus_tag="VMZ53_01180"
     CDS             9545..11698
                     /locus_tag="VMZ53_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27081.1"
                     /translation="MRVWLLVMLIGCGRIGFDARGDANSDSVGDGPVARCKFLDVATG
                     RDHTCAIDGDGSVFCWGRNDYGQVNGATSGPKTIPTQVTLPAAATRIAVGRDFSCANA
                     GGDVYCWGNNDLGQLGVGDVAERTGPVRINLGVAASDIVAGTFHGCAVGAADGQVFCW
                     GQNQHFAVGTATLDAVVPTPTALTGVTGVRAISLGHRHGCAVSTDGMTRCWGRDEFGQ
                     LATTPLNDHAPMIASNVSAVDVSAGGRHTCGVDAAGQLHCWGRNEAGELGIGSVDTGP
                     HATSSTMLLAGVTQIATGAYGTCAIHGGGTLSCWGDATRANGSFDVSPSPVDTPITDA
                     AKLSYGYFHACVIRASGELLCWGLDDTGELGRGKRSAAAAPVAATIATPTLIAAGGLH
                     VCAVVGADVLCWGHNPNGQLGNGTRNGTRTPTVIATGLTTIEGLSAGSNHTCAWGLGQ
                     VQCWGKVLGGIQLTPAFVAGFASVERVVSGDRHDCALGGGTVQCWGDNDRGQLGNNTM
                     TPSATPVTVNNISAATAMCAGTQHACAIDAGAVKCWGGNGAGELGDNTQTDRLQPVAP
                     GLTASSLACGRSHTCALDAGGDVYCWGRNLSGEIGLGTFTAPIKVPMKVTLPEKAVSI
                     SSGGYGTCARLMSGAVACWGASEQGEIGIGAFGTTAVPKLATGLANADLLAIGRSGGC
                     ARIGGVVKCFGDARMLGNGDNSDAVPMSPSFTCTQ"
     gene            11708..12469
                     /gene="truA"
                     /locus_tag="VMZ53_01185"
     CDS             11708..12469
                     /gene="truA"
                     /locus_tag="VMZ53_01185"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HUS27082.1"
                     /translation="MRHIRLVVEYDGSQLHGWQRQHGVPTVQEHLEIALAKLLTHEVQ
                     VTGASRTDAGVHARGQVASFRTERPIPVHGVRRGLNSLLPPDIAIRDASEVPEDFHPR
                     FSATGKHYRYTILARPERSPRWRDRAWHHSEPLSLPAMHDAARALIGEHDFAAFRAAG
                     CTAKTTLRRVDSIAFTRLHPHLLEVDIRGNAFLRQMVRIIVGTLTEVGAGRRPVSQVA
                     EILAGRDRTQAGVTAPAQGLELLEVRYDGTRIAPV"
     gene            12503..13126
                     /locus_tag="VMZ53_01190"
     CDS             12503..13126
                     /locus_tag="VMZ53_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS27083.1"
                     /translation="MASEPATAVELEEEQRQVEEAQAGNLDAMRPILERYAQPLYGTV
                     ILPRLGDAASAEDVLRDTLATAVEKIRRFTWQGKSIYPWLRQIAINKVYDVHRQAKRS
                     RRLADAMVHEVPVETDPDSHADAQLIADQERAAHRARIEEAMGLIADRYRLAIELRLV
                     QELTREECAKRMSVTIGTFDVLLFRAVRAFRKHFGERAAGQETTDGD"
     gene            13116..13715
                     /locus_tag="VMZ53_01195"
     CDS             13116..13715
                     /locus_tag="VMZ53_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27084.1"
                     /translation="MATKNDDDILTAPVPAPDDVASSSERMHAKTFGELVDKTIAGRT
                     PAAMSADDRALLEVATVIRAASGNAELPAGKRRSIVEDALRQAVGGVSASTSGAVTPI
                     SRGRARRWAPWAVAAGASLVAVAAVLLLWLRGPERVTVEAPVAHQVPTTWKSRPADPL
                     IGPISREAVGDASSRIDAIFADRLDGYRDRQFARGRKAP"
     gene            13712..14308
                     /locus_tag="VMZ53_01200"
     CDS             13712..14308
                     /locus_tag="VMZ53_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27085.1"
                     /translation="MKKYIPLVVLGLALAVTSVTACSCTGDRDRTQGDDRRPTVQTPD
                     PTDVIDQGLMIALSKAKNYHHKARVYMSDGKIAEAIAALREILSLKFPAGAPEADDVR
                     NDARALLAKLLVSQSQIDEAMRVVQEGIAQSQRESFFVANLYTVKGEIHEARAALLDA
                     NDPAQKAKIVDEKHAAIDAYDKSNSINTVLQDKLMETP"
     gene            14305..14769
                     /locus_tag="VMZ53_01205"
     CDS             14305..14769
                     /locus_tag="VMZ53_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27086.1"
                     /translation="MKTLLPVALVAMIVAFACGPKQPPEGPRKQNEIQTLWTQIRDWR
                     HEAGWDVDPRREDVVAVVGTPVKQIQQICPENHAVPQACSDVCSLADAICDNAEQICI
                     LADELGKQDTWAQDKCTSSKASCREAKKKCCGKCSETKVVPPPPAPAKDKAS"
     gene            14766..15239
                     /locus_tag="VMZ53_01210"
     CDS             14766..15239
                     /locus_tag="VMZ53_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27087.1"
                     /translation="MKQFIAASLCMVLVAACAAGGAQKSARVASPPQSADAQGSPVPT
                     NTMSAKDELDALFLAVERDRQVDQLPEAELDPASASTMPMAETNRSPKTDPTCKPAPS
                     ETCNTSCTLSGSICTNKDRICELAKDLGDADSIGKCNKATKTCKTAADKCCSCML"
     gene            15282..16667
                     /locus_tag="VMZ53_01215"
     CDS             15282..16667
                     /locus_tag="VMZ53_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27088.1"
                     /translation="MLRRCALVLVAVTATHARAESPVVGGRSARSIGRAGVGTASDDG
                     GGALLANPAAIARREAPRMQLAVAFLDDEMSWLHSASSPVNRDQSSSRLLPMLAIEGA
                     IGEWIVGAGVVTNTRTERLLRRPGINAPARDYGNTFEYRYDGFAGSFRRDMLVAGAAH
                     RIGDTVAIGLSVSASRVEVSELRRLWAGDTNRKPGGVNEVAGDPTQDVELGMTGSAYT
                     PGVVAGVLIAPESTSLEIAASASWMGPARATGEVTGDGAIPQSQYAVSFDSTAARLEI
                     EQPLTFRTGVRWLSERWIAELGGDLWVVPKRAAAPVWHFDGVRITDATTIGATREVQL
                     AALPSRMSTRTHGALRGALDVELISGFLWATAGYAFATSGTAGTRLSPTFGDLGGHTA
                     AIGLEATAGGFTVTFGAARTWSLRSPEPASAWRLDNPFGTGDAMVPVGTFDGSVDMIG
                     LSIDAELWAPE"
     gene            complement(16864..17316)
                     /locus_tag="VMZ53_01220"
     CDS             complement(16864..17316)
                     /locus_tag="VMZ53_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27089.1"
                     /translation="MIRLFVGLLKGAVIGGAVGYGAHALAEATGFSNAWLTYGVVGML
                     VGLFVGKPLWTLLRDPNTTNIVAIIKAVFGFGVGCGLYALFAKAWAPDPKALMIADYS
                     IYWPPILGGAIGAVYGGFVELDDSIGDDRSAKSAKSESGPVKKLPAKK"
     gene            complement(17409..18794)
                     /locus_tag="VMZ53_01225"
     CDS             complement(17409..18794)
                     /locus_tag="VMZ53_01225"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUS27090.1"
                     /translation="MVRRRTPKRKKRTTYHTLGPSLGAAPAKKNGAWVLLVLGALVVV
                     NLYVFVWDKKTSVAAIKQQAEAKGSGANPSMSMPAAPLVAGDARASAPVGPPGTIEGK
                     VGKSDTLGKLLKKSGLSAAEADEVIRSLADVLDFKSIKAGQPFRIERGPDGRVKLFEL
                     ELSKIQKVRAERQQSGELQGSADNKETRIELKSITGKIDSSLYASLKAAGETAALVDF
                     FADVFAYDIDFYNDTREGDTFHIVVEKKWSDIAGKQELVGYGRILAAEYTGKEGTYKT
                     FFWNGKYYDEAGQSSERGFLKTPLKFARISSGFDRARMHPILHVARAHLGVDYAAPVG
                     TPVWAAASGTVTHAGPAGGAGNLVMIRHDNGIETAYMHLSKFASIKVGQRIEAKTVIG
                     YVGTTGLSTGPHLHFGVKKNGVFIDPSKLAPWRAKSVTASERDAFKAEVAKLESQMTT
                     QAAPAPQVSPG"
     gene            18960..>20833
                     /locus_tag="VMZ53_01230"
     CDS             18960..>20833
                     /locus_tag="VMZ53_01230"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HUS27091.1"
                     /translation="MAKKSDKKAAKASKKIAVEAEAAEIAAPAEKKKRGKGNAGDGGG
                     GGGGGGGGGGGGGGALGPGMMDTSLEAEARRRYLNYALSVITSRALPDVRDGLKPVHR
                     RILYAMLHDQHLRPDAKYRKSATVVGAVLGRYHPHGDSSVYDAMVRMAQDWALRVPLV
                     DGSGNFGSLDGDEAAAYRYTECRLAPAAMELLRELDFETVEMRPNYDGTTEEPTVLPA
                     RFPNLLVNGSTGIAVGMATNIPPHNLREIVDALLALSKDRKIEHVTLMKYIDGPDFPT
                     GGLMLNNKVELRQIYKEGAGTIRIRGEYKVEEKKKGGSDIVITSIPYAMTKSDLVQKI
                     ADVIISRKMPFLLDVRDESTTDVRIVLELKKDADVDLVMGYLYKHTPLQSSFHVNLTC
                     LVPPELMDDGSVPTGAPPQPKKCGIKEMLEHFLDFRIITTTRRFEYELAQLKKRIHIL
                     EGYVTIFDALDELIKIIRASDGKEDAAQKIIKRFKLDDIQTDAILELKLYKLAKLEIN
                     VIREELEEKAKEAKRIQKILNDEDKLLDVVRGDLEEVAKAYGTPRRTKTGGASEEVEF
                     DADAFIIEEDANVVVTRDGWIKRVRELKDPTQTRTREGDAVTHVLPGSTREKVIFFTN
                     R"
BASE COUNT         3566 a         6815 c         6991 g         3461 t
ORIGIN      
        1 ggccgcgcga ttcccggcgt cgagatcgtc ggcgacgacc actaccggcg cgtcgtgcat
       61 ctcggtgggc acgtcggcga gattcgcgtc gagcccgcgg accgtggcac ggcacgcggc
      121 aagtcggggc aacgcgaaac gcggataacg cgaggcgagc gcggccaatc gccgaaggcg
      181 cgagctgcgt tgctgttgac cgtgtcgccg gcgttgctgc ccgtgctgat gccgctcgtg
      241 gcgcgcgtgc gacgcatgtt cgatctcgac gcgcatcctg atcgcattgc gagcgtgctc
      301 ggccgcgatc gcgcgctcgc gcgccatctc gcgaagcggc cggggttgcg cgtaccgggc
      361 gcgatcgatc cattcgaggc atcggtgcgt gcattgctcg gccagcaggt ctcggtcgct
      421 gctgcgacga cgctggcggg gcggttcgcg acgcagctcg gcgtggcgta tgcgggtggc
      481 gaggggctcg cgtatcgctt tccgactccg gcggaggtcg tcgcggcggg tgcggagcgc
      541 atcgcgaagc tcggcatgcc gggcaagcgc gggctcgcaa ttcatacgtt cgcgaaagcg
      601 atcgtcgacg gcacgattcg cctcgacgtc gcgaccgatc tcgacgcatt tgttgacaag
      661 ctcgtcgcgc tgcctgggat tggcccgtgg accgcgcatt atctcgcgat gcgtgcgctg
      721 cacttgccgg acgcatttcc tgcggcggat ctcggcgtgc agaaggcgct cgggcgcgcc
      781 ggcgcgaaag cggccgagaa gcgcgcagag gcgtggcggc cttatcgctc gtacgccgta
      841 cttcatctgt ggatgggact gggagactga ccatgatgct ctcgatgatc acgatggctt
      901 caccgctcgg gacgttgcgg ctctatgccg acggcgacga gctgaccgcg gtctcgttgc
      961 cctgtgacgc ggcagcggtc gcgaacgaat gcagcacgcc ggtgctcgac ctggctcgtg
     1021 cgcagctcac tgaatacttt gcggggacgc ggcgtgcctt cgagcttcca ctcgcgcctc
     1081 gcggcactgg ctttcagcag atcgtgtggc gcgcgttgac gacgatccgg tatggcgaga
     1141 cgtggtcgta tggccagctc gcgcgcgtga tcgggcggcc ggcggcgagc cgtgcagtcg
     1201 gcgcggcgaa tggacgcaat ccactcgcga tcatcgtgcc gtgtcatcgc gtgatcgggg
     1261 cgaacggcac gctgacggga tatgccggtg ggcttcccgc aaagaagtgg ctgctgcagc
     1321 acgaggtcgc gcacggcggg catgccgcga taggcacgtt gttcgccagc tgctagggtc
     1381 cgcatgtgaa ctggttcttc ctcggcgggc tcgccggcct tgcggggttg ctcgcgagcc
     1441 gcggcttgtt tcgccgcgcg aaccagtcgt cggcagcgcg tgcatcgggg ctcgagccac
     1501 ttggcgatct gacgcacatc ccgtcgtcgc tgcagaagac cgcgctgtgg gcgctcgcgg
     1561 acggcggctt cgagcgacgc gttgtcggcg gcatcctcgc gcgcgatgga ggcgacgtga
     1621 ccgtgactgc attcgatctc gagacgctgc gcgagcgccg cggcgagtgg gcgtggctgc
     1681 cgatcgagcc gccgtttcgc atcgcgccgg tggtcagcat cgtcgtgtgc gaggtcgagc
     1741 gcgcgttccc gcatatcttg ctgaagcgtc atggccacgg cgaccagatg gccgacgaca
     1801 ccgtgctcga gcgcgtcggc agcgtgtcga agctcgtccg cgatggcctc ggcatggcgc
     1861 gctcgtatgc cgccgagcta cccgcgccgc tgcccgcgac gcccgcgtcg acgacgctgc
     1921 ctgaaggctg gcgcgcgtac acgcaagcgc ccgacctcct cgagacgctg ctgccggcag
     1981 gcctcgccgc agcgctcgaa cgcgggggta ggcgggacct cgtcgtcgag ctgctcgacc
     2041 gtctcgtcgt cgtttatccc gcggctcgcg acgtcgtcgg cgcggaccag ttcgccgatc
     2101 tcacgacgac cgcgctcacg atcgtcgatg gggtgctcgc cgcctcaccg cctgtgacgc
     2161 cgcgcggcat cgacgtaaca acttcgtgac gccaacgatt gcgctcgcga gctgacctcg
     2221 cgggtcccta cgatgcgcag cgatgagagc aggggcgatt ctcgccgtgg cctgtgctgc
     2281 gtgcagcagc ggcaacggtc cggacatggc tgcacccgat gcgtcgtcga gcatcgacgc
     2341 ggttgcaacg actgatgcgc tgctgtcatc ggattccgac ggcgatgggc tcgcggacga
     2401 tctcgaagat cagctcgcgc gcgactacct gccattcgtc tcgctcgatc cgggcgacgg
     2461 ctgcacgctg tcaggcttcg tcgcgcgcgt gcgcaagcat cccgccgatc cgacgaagat
     2521 cctcatcgtc tactcgcatc tattccagcg cgactgcggc ctcggcggtc acattggcga
     2581 caacgaagcg ttcggcatcg cgatcgatcc cgcagtcccc gcaccgggcg gcatccaagc
     2641 gatccgcacc gcatcgcacc agaacacgcc gtgccagcgc acgaccgagt gcacgacgtg
     2701 ctcgaacgat tcgcgtgcgc gctgcgactt cgcaatcgac gccggcgcgc agtggccggt
     2761 gctctacgcg agcaaagaca agcacggcca atacgcgacg aagtcaggct gcccgctcat
     2821 cggcacgtgc ttcgatcaat gcacgctgaa ttctgcgcgc caggtcgcgc cgatcatcaa
     2881 tgtcggcgag cccgctcatc acatggtgac gaacctgacg gcgcagggct tcatcacacc
     2941 cgcgaacggt tggaccgagg cggagctgat gaacgtcgac ccgtggaatg cgatggtcga
     3001 cttcggcggc gccggcaaca tcgccggcga tctcgaggac tcgacgttcg agcccgcgcc
     3061 ctgccagtga tcggattgaa cacgaagaca cggagacacc gagcctgatt atcggatcga
     3121 tcttcctcga cccttgttct gtcttcgtgt cttcgtgtct ccgtgttgat tcgtccggcg
     3181 gtgccagccg gcgcggagag cgcgctagtt catgcgcgtg gcgaggtcgc cgaacgatgc
     3241 gttcacgcag gcgcggcccc acgtgacact cggcgtcagc gtcgcgggcg acgtctgcag
     3301 atagatcgga tcgcacgtca ggccgaccgg gcacgtcgcg tcttgcgtgc agagctcgac
     3361 gcagacatgg atgttcgcat cgcagaactc gctcgtgcag tcgctgttct gcgtgcacgc
     3421 cgcgccgccg cgacggttgt gcagacccgc gcccgcgttc ggacggcaca cgggcacgac
     3481 ttcgatcgag cgcttgcccg tgacgtccgt cggaacaccc gtggtccacg taccggtgac
     3541 gacgttgttg cccatgatcg agcagtggtg cgtacccgac gggcactgcg agttgtcgcc
     3601 gcatggcgcc gagcacgtac cgctcacgca cgacgccgtg ccccacttgc agccgcccgt
     3661 gcacgattga ccctggccct gcgtgccgtt cgccaggccg cacgacgcgt actggaacac
     3721 atcggcgagc gtgttctgct gcaggatctc gcagaccgtt ccagttgcgc agccgccgcc
     3781 gcccgtcgag tcgtgatcgc agaagtcgat gcagtacttc tgcgtctggc cgacttggag
     3841 gatcgcgcac gaaccgctac ggcattcgtt gtccgcgttg cacgtcgcgc cgccgagctt
     3901 cgtgccaagc gtcgccgtcg tctccgagca tgcgtgctga ttgtccgact cggtccagcg
     3961 gtactcgcac gtctgggtcg acgtacagcc agcgccgatc ggaccggtga agctgccctc
     4021 gcagaacgat gcgcagcggt agttgccggt gcagacgcca gtcccgtcga ggatgcagcc
     4081 gcactcgagg tcgtctttgc agacgtcgtt cgcggcccag ttgcattgcc cactcacgtc
     4141 gcagttctgc gtcgtgaact tgttgcagtc cgtgtcgaag ccatcgcacg cctcgggctt
     4201 gcccgggccc accgtcggct gcgcgtcatc gcaatcgcct tggcactcgg acttgccgtc
     4261 gccgtcgtta tcggtcgtgt cgcagggctg gcacgtctcg tcgatgtagc cgttgcagtt
     4321 ctcgtcgacg ccgttgccgg tgccggccgt gccacacagc tccgttgcgc ccggatggac
     4381 cgcggccgct gcatcgttgc agtctttgac gagcggatca tccgagcaca ccgagtagcc
     4441 gtcttgatcg agatcgtagt cttcgtcgat cttgccgtcg cagtcgtcgt cgagtccttc
     4501 acacgcgtgc tcggcggcct gcggcttcac gagtccgttg ttgtcgttgc agtcgacgca
     4561 ctggttcgag ccatcgaagt cggcgtcggt cgccgtgttg cacgtgccgc acgtgccgtt
     4621 cgagcagaac tgattcgtgc cgcacgaggt gccatccggc gcgcgcagat cgctggggca
     4681 ggtgttcgtc aggccgtcgc agacttcggc gaggtcacat gcgttcgccg cttgccggca
     4741 ctgcgtgttg aacggagcaa gcttgcagtt ctggcagcac gcgcccgtct cgcactgttc
     4801 gtcgccggtg atcatgccgt cgccacacgt ggcctgcacg acatcgacgc cgggcgcggc
     4861 atcaggcgtc acacctggct tgatgttgtc gccacaggcc gaaatcacga acaagacaag
     4921 caaggcaaac gctgcgcgca tggcgcgtcg tgtaccaccg tcggcgatcc gttgtcgatc
     4981 tccctctaag cgggatcggg cagattctcg acgacgcgct cgagcttgtt cagatcgatt
     5041 ggcttcacca ggtggtcggc aaacccggca tcggccgacc gctgcttgtc ttgctcctgg
     5101 tctcgcgcag ttacggccac aaagtgcagc tcgcgggacg ggaccttgag ctcgcgaagg
     5161 cgcttggaca gctcccagcc gtccatgacg ggcaggttga tgtcgagcag ggcgacgtca
     5221 ggctggaacg tgcgagccac ggtcagcgcc accggaccat tgtgggcaag ggcgacctgg
     5281 tagccgagcg tctcgagcgc gcgctggagc gcgaccgcgg tgtcgtggtt gtcctcgacc
     5341 agcaacactc gcttgcggcg ttcgaggttg atcggaggga tcagcgcgcc gtcgggtgcc
     5401 ggcgtatgcg gcagctcgat tacgacctcc gtaccgcttc cacctccgtc actgcggatc
     5461 gcgagcttgc cgctgtggag ctcgaccagc ttgcgcgaga tcccgagacc gagaccgagg
     5521 ccggcgttcg tcgggcgtgc accgttgaac ggttcgaaga tctgctcgag tgcgtgcgac
     5581 tcgatgccag ggccagagtc gcgaatgcga attcgcacgg actcgccgct tcgttccgct
     5641 tcgacgttca cgaccgatcc tggatcgctg tacttcgccg cgttgtgcag gacgttcgtg
     5701 aacacctgcg ccatccgctc tggatcgcaa tcgacgccga gaccgctgcg cggaacgcgc
     5761 acgaacacct gcgtcttggt ctcctcgaac gtgcgcgaga gacgctcgag agacagatcg
     5821 atcacctgcg cgatctcgag cgtagtccgc cgcagctcga gcttgccgcg gcgcaagcgc
     5881 gacgcatcga ggagatcatc gacgagccgt gtaaggtgtc gaatcgatcg gtcgatcacc
     5941 atcacttcaa ctgacggcga ttgccgcacg agaacctgca gcgatgcagt gatcgccgac
     6001 agcgggttgc gcagctcgtg gccgaacagc gacaggaaat catctttcgc ccgctgctcc
     6061 acatcgacgc gtgcgccatc gtgcagctcg acgagaactc cctcggcgcg aacaccctcg
     6121 ctgtagatcg gcgtcagcgt gaacgtgacg ttgagaatgt cgtgtgcgag ctcctctgcc
     6181 gcggccgggt cctggcccgc gagcacgaca tcgaccgcgt cgacgaggct tcgccacggc
     6241 tgcagcgatc ttccggagcg agcgattgcg ctcgtgtggt cgatcaaacc gatgagcttt
     6301 gcacacgctt cgttgtagag gagcgtgtgc tcgatgcccc aaacgatcgc catcggctgt
     6361 ttggatgcga agcaaatgct cgccgcggtg cgaaggctca ccggccacgt cgtcggagaa
     6421 ccgagcggcg tgtcgtccca ctggaattct cgcagtcgca ctgcgagcgc ggtatcgccg
     6481 gaaaaaacgc gacgaagatc atcgttcgac atacgcgagg acatcaccat ggactatggt
     6541 cctctcgcaa acggcgtaga gctatgttgg tcatcattca ctcagggcag actggcgttg
     6601 aacgcggcgc gcatcgcgcg gcactcgcgt caggtctctc catagcgggt ttcatgccgc
     6661 tcgaccggcg tgacgagctc gggccgattc caccggacgt cgcgggaagc ttgacgcctt
     6721 gtttcgatcg cgggccgcgg ccagcagtcc gggccaacat ctcgctcgca tccggcgtct
     6781 tgctcgtcgt tccgaatgcg gcgatgccgg agaagttcac tgcgatgagt gcagtactcc
     6841 agggcattcg ttctgcgcac tgtccgtcgc tcatcgcaga ctccgccgtg aacctcgatg
     6901 acgtcgcgaa atgggcgacg acgctgcccg agacgagcgg ctcgacgcgt atccacgtga
     6961 cgggcccccg cagcactcgc tggcccgagg gcgagagcct ggcgcgccgg ctcgttgcgg
     7021 cgatcgccat gtcgcagtaa tcacgagcga aactcgctca gatcgatgcg cgccggcgtg
     7081 cgtgtaatgt cgcaaggcat ggtgcgtccg ctgctcgccc tcgtcctcgt tctcgcatct
     7141 gcgagcgcgg cgactgcagc gagcgagctg cccggcaaag cgggcatgta tccgcagcaa
     7201 ggtgcgccgc tcgcgatgct cgacagcaag gtcgtcgtgc gcgtccatgg gccgatcgcg
     7261 gaagcggtcg tcacgcagac atttcgcaac gacaccgacc gcgtgaccga ggcgacgtac
     7321 atctttccgc tgccacttga tgccgccgtg acggccatgg agatcgagtc cggaaaccgg
     7381 accattcgcg cctcgatcga gacccgcgat caggcgcagc agcgctacga gcgcgcggtt
     7441 gcggaaggcg tcggcgcgag cctgctcgag caagagcgcc ccgacatctt cacgcagacc
     7501 gtctcggcga tccccgcgcg cggcaaggtg accgtcacat tgcggttcga cacggtcgct
     7561 cgctacagcg ccggcacgtg ggagctcgca ttgccgctcg tgatcgcgcc acgctacgtg
     7621 ccgggcaccg cgagcggacg cccgacgact ggcagcggcc gcgcgcctga taccgaccgc
     7681 gcacccgatg cgtcgcgcgt gacgccgagt ggctcgccgg gtgcgggcgg gtcgacagaa
     7741 gtcacgctcg agcttgccga agtcgatgac gtcacgagcc cgacgcacga gctcgtcaag
     7801 cagaagggca gctaccagct cgtcgatccc aagagcgatc acgatgcaat cattcgctgg
     7861 cgcgcgaaag cgccgacggc tgcgtgggtg gaagcggatg atggcggcgg ctacgcggcg
     7921 atcgtcgtcg aggcgaagcc ggcagcgaag ccggcgaccg cggtgaagat gctgctcgtc
     7981 ctcgatcatg cagcgacgac gcgcggcgat gcagacgccg tgaagaagcc gctcgtccgc
     8041 gcgctgctgc aaacactcac cccgaaggac cgcgtgcgcg tcgccggcag cgactcgctc
     8101 gacacgcgag cacccgccga tgcgtggaaa gcgctcgacg aagcgtgggc gaagccgcat
     8161 ggcgctttcg atctcgcgaa ggtgctggcg cgcagcgacg atccaatcgt tctcgtgacc
     8221 gacggtctcg tcgccgacga tcgcgcggtg ctcgcagcgg ccgcaaaggt cggcgcgccg
     8281 gtgcacgtaa tcggcgtggg tcctgcgccc aaccgcagct tgctggccgc gatcgcgacg
     8341 acgacgggcg ggacggttcg ctacgcggtc gtcggcgatg atgcgcaagc gatcgcacgc
     8401 gatgtgctcg ccgacatcgc gacgcagcca gagccgctga cgatcaactg gggcacgctc
     8461 gcggcaagcg atgtcgtacc tgcgacgctg cctcgcctcg gcgcgaatca agcgatgctc
     8521 gtgctcgcgc gcgtcaagaa agtccagtcg gcgaacgcgc gcgtgcgcgg cgacgtgttc
     8581 ggcttcaccg ccgtcacctc gagcaagacg ccggacggcg cgaccaccac gcacggcgca
     8641 ctcgctcgcc ggtgggcgaa gctcaagctc gacgatctcg tggcagcagg caacgcgaag
     8701 tcgatcaccg agcacgcact gcgcttcggc ctcgtctctc catacacggc gatggtcgcg
     8761 atcggcgacg aagtcgtcgt gcaaggcggc gtcaagcaca gcgttgccgt tcccgtctcc
     8821 gtgcccgccg gcatgcagtg gcaacacgtc aagcgcgaga tcacggtcga tacgacgaag
     8881 acgacgcagg acttcagcag catgaagctc gacgaggacg agaagcctgc gaagaagaag
     8941 ccgcgtcgcg agtccgaaga tgtcgttggc ggcgaagcgt ccggcgccgg ctcgacgaag
     9001 gacttcggcg acgaggaatc ggatggagcg ccgccggaga atgcgccggt ctcgccgtcc
     9061 tcggttgcga tgggcatgga ttacgaggaa gaagcggacg atgccgagag catcgaggtt
     9121 cgtggctacc gtagccgcac gcgctactcg ctcggcctcg gtggtgggct ctcgatcgtc
     9181 aatggcacgg cgtcgcctgc gttcgagctg tcggcgcgcg tcgagcgact cggcaggttg
     9241 cgcctcggcg gcgaagcgtc gctgtggctc gtcgatggtc tgcacggcca gggtcacttc
     9301 ttcgccacga tcggcaaggc cctcctgcct cgcttcgatc tgcgtggcgg tccaggcctg
     9361 acgctcaccg gtgacggcgt cggtcctgcc gtcaacgtga cgctgcgtgg tcgcgtttct
     9421 cgcaacgccg ccgcatacct ccgctacgac ggcgcactgc tcattcgcag cggcgcgtac
     9481 gacggccaga acaccggctc gttcggcatc gaagccagct tctaagcact gataagatcc
     9541 acgcgtgcgc gtgtggttgc tcgtgatgct gatcggctgc ggccggatcg gcttcgacgc
     9601 gcgcggcgat gcgaatagcg attctgtcgg cgatggccct gtcgcgcgct gcaagttcct
     9661 cgatgtcgcg accggtcgcg atcacacgtg cgcgatcgat ggcgacggca gcgtgttctg
     9721 ttggggccgc aacgactacg gtcaggtcaa cggagcgacc tctggaccga agacgatccc
     9781 gacacaggtg actcttcccg cggccgcgac acgcattgcg gtcggccgtg acttctcatg
     9841 cgcgaacgcc ggtggcgacg tgtactgctg gggcaataac gatctcggtc agctcggtgt
     9901 cggcgatgtc gctgagcgaa ccggccctgt tcgaataaat ctcggcgtcg cggcgagtga
     9961 catcgttgcg gggacctttc acggatgcgc ggtcggcgcg gcggatggcc aggttttctg
    10021 ctgggggcag aaccaacact ttgcagtcgg aacggcaacg ctagacgcgg tggtaccgac
    10081 gccgacagct ctgacgggcg tcaccggcgt ccgagcgatc tcacttggac atcgtcatgg
    10141 ctgcgcggtg tccaccgacg gcatgacacg ttgttggggg cgcgatgagt tcggtcagct
    10201 agcgacgacg ccgctgaacg atcacgcacc gatgatcgcg tcgaacgtca gcgcggttga
    10261 cgtatcggca ggtgggcgtc acacgtgcgg cgtggatgcg gccgggcagc tgcactgctg
    10321 gggacgcaat gaagctggcg agctcggcat cggtagcgtc gatacggggc cgcacgcgac
    10381 gtcgtcgacg atgttactgg cgggtgtcac ccagatcgcg accggtgcgt acgggacgtg
    10441 cgcgatccac ggaggcggca cgctctcctg ttggggcgac gcgactcgag cgaacggcag
    10501 tttcgacgtc tcgccatcgc cggtcgacac gccgatcaca gacgccgcga agctctcgta
    10561 cggctacttc cacgcgtgcg tcatccgcgc ttctggcgag ctcttgtgtt ggggactcga
    10621 tgacaccggc gagctcggtc gtggcaagcg aagcgctgcc gctgcgcccg ttgccgcaac
    10681 gatagcaacg ccgaccttga tcgcagcggg cggcttgcac gtctgcgccg tcgtcggtgc
    10741 cgacgtgttg tgctgggggc acaatccgaa cggccagctc ggcaacggaa cgcgcaacgg
    10801 aacgcgcacg ccgaccgtca tcgccacggg cctcacgacg atcgaaggcc tgtccgcggg
    10861 atcgaatcac acgtgcgcgt ggggactcgg tcaggttcaa tgttggggca aggtgctcgg
    10921 cggcatccag ttgacacccg cattcgtcgc gggattcgcg agcgtcgaac gcgtcgtgtc
    10981 gggcgatcgc cacgactgcg cgctaggcgg cggcaccgtg cagtgctggg gcgacaacga
    11041 tcgcggccag ctcggtaaca acacgatgac gccatcggca acgcccgtga cggtcaacaa
    11101 catttcggcc gccacggcga tgtgcgccgg tacacaacac gcgtgcgcca tcgacgctgg
    11161 tgcagtcaaa tgctggggcg gcaacggagc cggcgagctc ggcgacaaca cgcaaaccga
    11221 tcggctgcag cccgtcgcgc ccggcctgac cgctagctcg ctcgcgtgcg gccgcagcca
    11281 cacgtgcgcg ctcgacgcag gcggcgatgt gtattgctgg gggcgcaacc tgtccggcga
    11341 gatcggcctc ggcacgttca cagcgccgat caaggtgccg atgaaagtca cgttgcccga
    11401 gaaggccgtc agcatcagct cgggaggcta tggcacgtgc gcgcgattga tgtccggcgc
    11461 cgtcgcttgc tggggcgcga gcgagcaggg cgagatcgga atcggagcct tcggcacgac
    11521 cgccgttccc aagctagcga cgggtctcgc gaacgccgat ctgctcgcaa ttggccgcag
    11581 cggcggctgc gcacgcattg gcggcgtcgt gaagtgcttc ggcgacgcgc gcatgctcgg
    11641 caatggcgac aacagtgatg ccgtcccgat gtcgccgtcg ttcacctgca cgcagtagac
    11701 taagcgcgtg cgccacatcc gactcgtcgt cgagtacgac ggcagtcagc tccacggctg
    11761 gcagcgccag cacggtgtcc cgaccgtgca ggagcatctc gagatcgcac tcgcgaagct
    11821 gctgacccac gaagtccaag tcaccggcgc atcgcgcacg gatgccggcg ttcatgcgcg
    11881 cgggcaagtc gcgagctttc gcaccgagcg gccgattccc gtgcatggcg tgcggcgcgg
    11941 gctcaactca ctgctgcctc ccgacatcgc gattcgcgac gcgtccgagg tgcccgaaga
    12001 ttttcatccg cggttctcgg cgaccggcaa gcactaccgc tacacgatcc tcgcgcgtcc
    12061 cgagcgttcg ccgcgctggc gcgatcgcgc gtggcatcac tccgagccgc tctcgctgcc
    12121 cgcgatgcac gatgcggcgc gagcgctgat cggcgagcac gactttgcgg cgtttcgcgc
    12181 ggcgggctgc acggccaaga ccacgctgcg gcgcgtcgat tcgatcgcct tcactcgcct
    12241 gcatccgcac ctgctcgagg tcgatatccg gggcaacgcg ttcttgcggc agatggtccg
    12301 catcattgta gggacgctga ccgaggtcgg ggcaggccgg cgcccggtca gccaagtggc
    12361 cgagatcctt gcaggtagag accgaaccca ggccggcgtc accgctcccg cgcagggcct
    12421 ggagctgctc gaagtccgtt acgacgggac tcggatcgca cccgtgtaag atgccgccgg
    12481 gattagcgtt ggaagagtgt cggtggcctc cgaaccagcc acagcggtag agctcgaaga
    12541 agagcaacgc caggtcgagg aggcccaagc cggcaacctc gacgccatgc gacctatcct
    12601 cgaacgctac gcacagccgc tgtacggcac ggtgatcttg ccgcgtctcg gcgatgccgc
    12661 gtcggcggag gatgtcctgc gcgacacact cgcgaccgcg gtcgaaaaga tccggcgctt
    12721 cacgtggcag ggcaagtcga tctatccgtg gctccgccag atcgcgatca acaaggtcta
    12781 cgacgttcat cgccaagcga agcgctcgcg gcgtctcgcc gacgcgatgg tgcacgaggt
    12841 gccggtcgag accgatccgg actcgcacgc cgacgcgcag ctcatcgctg atcaagagcg
    12901 cgccgcgcat cgcgcacgca tcgaagaagc gatggggctc atcgccgatc gctaccgcct
    12961 cgcgatcgag cttcgcctcg tgcaggagct cacgcgcgaa gagtgcgcga agcggatgag
    13021 cgtcacgatc ggcacgttcg acgtccttct cttccgcgcg gtgcgcgcgt tccgcaaaca
    13081 tttcggcgag cgagccgcag gccaggagac caccgatggc gactaagaac gacgacgaca
    13141 tcctgacggc cccggttccc gcacccgacg acgtcgcctc gtcgagcgag cgcatgcacg
    13201 ccaagacgtt cggcgagctc gtcgacaaga cgatcgcggg tcgcacgccg gcggcgatgt
    13261 cggccgacga tcgcgcgcta ctcgaggtcg cgaccgtcat tcgcgcggca agcggcaacg
    13321 cagagctgcc cgctggcaag cgccgctcga tcgtcgagga tgcacttcgc caagcggtcg
    13381 gcggtgtctc cgcatcgacg agcggggcgg tcacaccgat ctcgcgtggg cgcgcgcgcc
    13441 gctgggctcc atgggcggtc gccgctggtg cgtcgctcgt cgcggtcgcc gcggtgctgc
    13501 tgctgtggct gcgcgggccg gagcgcgtca ccgtcgaggc acccgttgcc caccaagttc
    13561 cgacgacgtg gaagtcacgt cctgctgatc cgctgatcgg tccgatctcg cgcgaagccg
    13621 tgggtgatgc gagcagtcgc atcgacgcga tcttcgccga ccggctcgac ggctaccgcg
    13681 accggcaatt cgcgcgcgga aggaaggcgc catgaagaag tacatcccgc tcgtcgttct
    13741 cggtctcgcg ctcgcggtga cgtccgtcac ggcgtgctcg tgcacgggtg atcgcgatcg
    13801 cacgcaaggc gatgaccgcc gtccaaccgt gcaaacgccc gacccgacag acgtcatcga
    13861 ccaaggcctg atgatcgcgc tgtcgaaggc aaagaattat caccacaaag ctcgcgtcta
    13921 catgagcgat ggaaagatcg ccgaagccat cgccgcgctc cgcgagatcc tctcgctcaa
    13981 gttcccggca ggcgcccccg aagccgacga cgtccgcaac gacgctcgcg cgctgctcgc
    14041 gaagctcctc gtctcgcaaa gccagatcga cgaagccatg cgcgtagtcc aagaaggcat
    14101 cgcccagagc cagcgcgaga gcttcttcgt cgcgaacctc tacacggtca aaggcgagat
    14161 ccacgaagcg cgcgcggctc tcctcgatgc gaacgacccg gctcagaaag ctaagatcgt
    14221 cgacgagaag cacgccgcga tcgacgccta cgacaagtcg aactcgatca acacggtgct
    14281 ccaagacaag ctgatggaga cgccgtgaag acgctgctcc ccgtcgcgct ggtcgcgatg
    14341 atcgtcgcgt ttgcgtgcgg cccgaaacag ccgccggaag gcccgcgcaa gcagaacgag
    14401 atccagacgc tctggacgca gatccgcgac tggcgccacg aagcgggctg ggatgtcgat
    14461 ccgcgccgcg aggatgtcgt cgccgtcgtc ggtacgccgg tcaaacagat ccagcagatc
    14521 tgccccgaga accacgcggt gccgcaagca tgcagcgacg tctgctcgct cgccgacgcg
    14581 atctgcgaca acgccgaaca gatctgcatc ctcgccgacg agctcggcaa gcaggacacc
    14641 tgggcgcaag acaagtgcac gagctccaaa gcgagctgcc gcgaagcgaa gaagaagtgc
    14701 tgcggcaaat gcagcgagac gaaagtcgtg ccgccaccac cggcccccgc aaaggacaag
    14761 gcgtcatgaa gcagttcatc gccgcgtcgc tctgcatggt tctcgtcgcc gcgtgcgcgg
    14821 ccggcggcgc gcagaagtcc gcgcgcgtcg cctcgccgcc gcagtcggcc gacgcacaag
    14881 gctcgcccgt cccgacgaac acgatgtctg caaaagacga gctcgacgcc ctcttcctcg
    14941 cagtcgagcg cgaccgccag gtcgaccagc tgcccgaagc tgagctcgat cccgcatccg
    15001 cctcgacgat gccgatggcc gagacgaacc gttcgccgaa gacggacccg acgtgcaagc
    15061 cggctccgag cgagacgtgc aacacgagct gcacgctgtc gggctcgatc tgcacgaaca
    15121 aagatcgcat ctgcgagctg gcgaaagacc tcggcgacgc cgactcgatc ggcaagtgca
    15181 acaaggcgac gaagacgtgc aagaccgctg cagacaagtg ctgcagctgc atgctctagc
    15241 gtaggccaca cacgtaggcg gcgacgcgct atcgtcgcgc tgtgctccgc cgctgtgcgc
    15301 tcgtgcttgt tgccgtcacg gcaacgcatg cgcgcgccga gtcaccagtt gtgggcgggc
    15361 gctcggcacg ctcgatcgga cgcgccgggg tcggaactgc atcggacgat ggcggtggcg
    15421 cattgctcgc aaatcctgcg gcgatcgcgc ggcgcgaggc acctcgcatg cagctggcgg
    15481 tcgcgtttct cgacgacgag atgtcgtggc tgcattcggc gagctcgccc gtcaatcgcg
    15541 atcaatcgag ctcgcggctg ttgcccatgc tcgcgatcga gggcgcgatc ggcgagtgga
    15601 tcgtcggcgc tggggtcgtc acgaatacgc gcaccgagcg gctgcttcga cggcccggca
    15661 tcaacgcgcc cgcgcgcgat tacggcaaca cgttcgagta tcgctacgac gggttcgccg
    15721 gatcgtttcg ccgcgacatg ctcgtcgccg gagctgcgca tcgcatcggc gatacggtcg
    15781 cgatcgggct ctcggtctcg gcgtcgcgcg tcgaagtgag cgagctgcgt aggctgtggg
    15841 ctggagatac gaaccgcaag cccggcggcg tgaacgaagt ggcgggcgat ccgacgcagg
    15901 acgtcgagct cggcatgacg ggcagcgcgt atacgcccgg cgtcgtcgcc ggagtcctga
    15961 tcgcgcccga gtcgacgagc ctcgagatcg cggcatcggc atcgtggatg ggccctgcgc
    16021 gggcgaccgg cgaggtgacg ggggacggcg cgattccgca gagccagtac gcggtgtcgt
    16081 tcgactcgac ggcggcgcgc ctcgagatcg agcagcctct gacctttcgc accggcgtgc
    16141 gctggctgtc ggagcgatgg atcgcggagc tcggcggcga cttgtgggtc gtaccgaagc
    16201 gagcagctgc accggtctgg cacttcgacg gcgtgcggat caccgacgcg accacgatcg
    16261 gtgcgacgcg cgaggtacag ctcgccgcgc tgccgtcgcg gatgtcgacg cgaacgcatg
    16321 gcgcgcttcg cggtgcgctc gacgtcgagc tgatctctgg cttcttgtgg gcgacggccg
    16381 gctacgcgtt cgcgacgtcg gggaccgcgg gcacgcgact gtcgccgacg tttggcgatc
    16441 tcggcggtca caccgcggcg atcgggctcg aggcgacggc cggcggtttc acggtgacct
    16501 tcggcgcggc gcgcacgtgg tcgctgcgat cgcccgagcc cgcgagcgcg tggcggctgg
    16561 acaacccgtt tggcaccggc gacgcgatgg ttcccgtcgg aacgttcgac ggctcggtcg
    16621 acatgatcgg gctatcgatc gacgccgagc tgtgggcgcc ggagtagcga cgatcagacg
    16681 aggctgaaga cggcgaccgc gacgaggcag aggtggccga ggacgaagag ccggatgtcg
    16741 cgcatgcgcg gcattaaaag cagatggcgt gccgcactca accccgcgag acttgcagtg
    16801 tctcaccggc tgatgcggta gccaggcagc aggagtgcat ggcggcccga caggccggta
    16861 tcgctacttc ttggccggga gcttcttgac cgggccggac tcggacttcg cggacttagc
    16921 gctgcgatca tcgccaatcg agtcgtccag ctcgacaaag ccgccgtaga ccgcgccgat
    16981 cgcgccgccc aggatcgggg gccagtagat gctgtagtcg gcgatcatga gcgccttggg
    17041 atcgggggcc cacgctttcg cgaacagagc gtacaggccg cagccgacgc cgaagccgaa
    17101 caccgctttg atgatcgcca cgatgttggt cgtgttcgga tcgcggagca gcgtccacag
    17161 cggcttgccc acgaacaggc cgaccagcat gccgaccaca ccgtaggtca gccacgcgtt
    17221 cgagaagccg gtcgcttccg cgagggcgtg cgcgccataa ccaaccgcgc cgccaatcac
    17281 tgcgcccttc aagaggccga cgaagagcct gatcacttat caagcatgac gaggaagttg
    17341 ggcatcggca aggccgatcg acaccgacga caacgtggca ttttgtgatg gctgcgacgc
    17401 cgtgcgactc agcccggtga cacttgcgga gcgggcgcgg cctgcgtcgt catttgcgat
    17461 tcgagctttg ccacctcggc cttgaatgcg tctcgctcac tcgccgtcac agacttggcg
    17521 cgccatggcg cgagcttgga cgggtcgata aagacgccgt tctttttcac gccgaaatgg
    17581 aggtgaggac cggtcgacaa acccgtcgtc ccgacgtagc cgatcaccgt cttggcctcg
    17641 attcgctggc cgactttgat gctggcgaac ttggaaaggt gcatgtaggc cgtctcgatc
    17701 ccgttgtcgt ggcggatcat gacgaggttt ccggcgccgc ctgcgggccc ggcgtgcgtg
    17761 acggttcccg acgcggcggc ccacaccggc gtgccgacgg gtgctgcgta gtcgacgccg
    17821 agatgcgcgc gcgcgacgtg caggatggga tgcatgcgtg cgcgatcgaa accgctcgag
    17881 atgcgagcga acttgagtgg cgtcttgagg aagccgcgct cgctcgattg gccggcttcg
    17941 tcgtagtact tgccgttcca gaagaacgtc ttgtacgtgc cttctttgcc cgtgtactcg
    18001 gcagcgagga tgcggccata gccgacgagc tcctgcttgc ctgcgatgtc gctccacttc
    18061 ttctcgacca cgatgtggaa cgtgtcgcct tcgcgcgtgt cgttgtagaa gtcgatgtcg
    18121 tacgcgaaca cgtcggcgaa gaaatcgacg agcgcggcgg tctcgcccgc ggctttgagc
    18181 gacgcgtaga gcgacgagtc gatcttgccg gtgatcgact tcagctcgat gcgggtctct
    18241 ttgttgtcgg cgctgccttg gagctcgccg ctctgctggc gctcggcgcg gaccttttgg
    18301 atcttggaga gctccagctc gaacagcttc acgcggccgt cgggaccgcg ctcgatgcgg
    18361 aacggctgac ccgctttgat cgacttgaag tcgaggacgt cggcgagcga gcggatgact
    18421 tcgtcggctt cggcggccga cagaccgctc ttcttgagca gcttgccgag ggtgtcggac
    18481 ttgccgacct tgccttcgat cgtgccgggc gggccgaccg gagcagaggc acgtgcgtct
    18541 ccggcgacga gcggtgcggc gggcatcgac atgctcgggt ttgcgcccga gcccttcgct
    18601 tcggcctgct gcttgatcgc ggcgacgctg gtcttcttgt cccagacaaa tacgtacaga
    18661 ttcacgacga cgagcgcacc gaggacgagc agcacccacg ccccgttttt cttcgcgggc
    18721 gcggcgccga gtgacggtcc cagcgtgtgg tacgtggtcc ttttcttgcg cttgggcgtt
    18781 cgtctgcgga ccatggcggt gaagaggccc agcaatatca cgggtctcga tgtcggacca
    18841 acaatcgata ttcggagcgc cagaagaaca tggcacagag cccaggcgat ctgcccccgc
    18901 gcgagcagct ggaacttttc atccagtcgg ggcaaggtgc gacggaacta gagcgagata
    18961 tggccaagaa gtccgataag aaagccgcaa aagcctcgaa gaagatcgcg gtagaggccg
    19021 aggccgcgga gatcgcggca cctgccgaga agaagaagcg cgggaagggc aacgcggggg
    19081 acggtggtgg tggtggaggc ggcggtggag gcggcggcgg tggtggtgga ggcgccctcg
    19141 ggcccgggat gatggacacc tcgctcgagg ccgaagctcg tcggcgatat ctcaattacg
    19201 cgctgtccgt gatcacgtcg cgcgcgctgc ccgacgttcg cgacggcttg aagcccgtgc
    19261 accgccgcat cctctacgcg atgctgcacg atcagcatct gcgcccggac gccaagtatc
    19321 gcaagagcgc gaccgtcgtc ggtgcagtgc tcggtcgtta ccatccgcac ggcgatagct
    19381 cggtctacga cgcgatggtt cgcatggcgc aagactgggc gctgcgcgtg ccgctcgtcg
    19441 acggctccgg caacttcggc tcgctcgatg gcgacgaagc cgctgcgtat cgctacaccg
    19501 agtgtcgcct cgcgcccgcc gcgatggagc ttctgcgcga gctcgatttc gagaccgtcg
    19561 agatgcggcc gaactacgac ggcacgaccg aggagccgac ggttctgccg gcgcgctttc
    19621 cgaacctgct cgtcaacggc tcgacgggca tcgccgtcgg catggcgacg aacatcccgc
    19681 cgcacaactt gcgcgagatc gtcgatgcgc tgctcgcgct gtcgaaggac cgcaagatcg
    19741 agcacgtcac gttgatgaag tacatcgacg ggcccgactt cccgacgggc ggcctgatgc
    19801 tcaacaacaa ggtcgagctg cgccagatct acaaagaggg cgccggcacg atccgcattc
    19861 gcggcgagta caaggtcgag gagaagaaga agggcggctc ggacatcgtc atcacgtcga
    19921 ttccctacgc gatgacgaag tcggatctcg tgcagaagat cgccgacgtc atcatctcga
    19981 ggaagatgcc gttcctgctc gacgtgcgcg acgagtcgac caccgacgtc cgcatcgtgc
    20041 tcgagctgaa gaaggacgcc gacgtcgacc tcgtgatggg gtacctctac aagcacacgc
    20101 cgctgcaatc gagcttccac gtgaacctca cgtgtctcgt gccaccggag ctgatggatg
    20161 acggcagcgt cccgacgggc gcaccgccgc agccgaagaa gtgcggcatc aaggagatgc
    20221 tcgagcactt cctcgatttc cgcatcatca cgacgacgcg ccgcttcgag tacgagctcg
    20281 ctcagctcaa gaagcgcatc cacatcctcg agggctacgt cacgatcttc gatgcgctcg
    20341 acgagctcat caagatcatc cgcgcgagcg acggcaaaga agacgccgcg cagaagatca
    20401 tcaagcgctt caagctcgac gacatccaga ccgatgcgat cctcgagctg aagctctaca
    20461 agctcgccaa gctcgagatc aacgtcatcc gcgaagagct cgaggagaag gcgaaggaag
    20521 ccaagcgcat ccagaagatc ctcaacgacg aggacaagct gctcgacgtc gtgcgcggcg
    20581 atctcgaaga ggtcgccaag gcgtacggca cgccacgccg cacgaagacc ggtggcgcga
    20641 gcgaagaggt cgagttcgac gcggacgcgt tcatcatcga ggaagatgcg aacgtcgtcg
    20701 tcacgcgcga tggctggatc aagcgcgttc gcgagctgaa ggatccgaca cagacgcgta
    20761 cgcgcgaagg cgatgcggtc acgcacgtgc tgccgggctc gacccgcgag aaggtcatct
    20821 tcttcacgaa ccg
//
LOCUS       DATLBZ010000025         5434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3746269, whole genome shotgun sequence.
ACCESSION   DATLBZ010000025 DATLBZ010000000
VERSION     DATLBZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3746269"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="VMZ53_01235"
     CDS             <1..2066
                     /locus_tag="VMZ53_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HUS27092.1"
                     /translation="PQKLYLPPIIDPEYHYEAVNVEAQQGNPSSLLWWTKRLIAKRKE
                     HKLFGRGSIEFINGDNPRVLAFVREYEGDTVLVVANLSRFVQCARLNLDRFRGSSPVE
                     LFGRMRFPEIPETPYFMSLGPHDFYWLQLEKPQAADLAVDRPTLSARRGWTELLEPAR
                     RRDLANALIRYASQRRWFRSKSRQRKEATITDIIRLDGEPRFAIALLHVEYAHGRPET
                     YVVPLAFAEDAEPHESMRSPGLVIANVTLSDVPSRGHVSGVLYDALCADTFGAALLRA
                     MTQGTGGKGERGTFSGSALAALQDLPADASLVPRLSSAEQTNSSVIFGDKLMLKIFRV
                     IEEGPSLEYEMGHFLASREPPYKGVPRLAGALEYVGHGRDSSTVGTLAELVPNQGDAW
                     QFTLDALDRYFDGVLSSDKRAMQPPDQQGSLIQRARLAPSDSVLDLVGAYIDRVRLLG
                     LRTAELHLALASDTSDPLFRPEAYDIMHQQSMYGSASAQMARTFDVLRKKRGTLSPDA
                     HTMADELIAHESDIDRLLERVTKRRLDVARIRVHGDYHLGQVLWTGDDFLIIDFEGEP
                     GRPLSQRRFKRSPLRDVAGMLRSLEYASAAALRDGRHRPEDMPVLSVWARAWTEWVSA
                     SFLGGYLDRVAGTKLIPPNDADTEQLLRFFMFEKVIYEIGYELDNRPEWVEIPMRGLL
                     TLLKP"
     gene            2063..3937
                     /gene="glgB"
                     /locus_tag="VMZ53_01240"
     CDS             2063..3937
                     /gene="glgB"
                     /locus_tag="VMZ53_01240"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HUS27093.1"
                     /translation="MKPQFGELDAHLFREGKHPRLHEKLGSHPDAKGTYFAAWAPHAA
                     AVSVIGDWNAWKPNVNALEPIGDTGVWHGYVKGVAKGALYKLHVRSKVRGYEVAKADP
                     FALRHEEAPGTASIVWTMDYSWGDAAWMKSRDNVSATNAPVSIYEVHLGSWMRVPEDK
                     NRSLTYREITPKLVEDVTKLGFTHVELMPLTEHPFFGSWGYETTGYFAATSRYGTPED
                     LMALIDALHAAGVAVILDWVPAHFPTDEHGLVYFDGTALFEHPDRKLGFHPEWNTSVF
                     DFGRQEVRSFLLSSALFWLERFHEDGLRVDGVASMLYRDYARKPGEWIPNDKGGNEYF
                     EAVELLQRLNDAIHREQPGTVTIAEESTAWPKVTRNPSDGGLGFSYKWDMGWMADTLR
                     YFARDPIHRQHHHNELTFRGVYAFSERFVMPLSHDEVVHGKGSILAKMPGDRWQKLAN
                     LRLLYAYQWSQPGKKLLFMGDEFAQWNEWNHDSSLDWHLLSDAGHRQIQLLVGELNRL
                     YRTEPALHELDCEPAGFQWLIPDDAVNSVLAYERISKGDQRIVCALNLTPVPRFNYRV
                     GVTQPGQWSEILNTDATELGGSGHGNLGRVEAMPVQMNGRPFSLTLTLPPLSAVFLRR
                     "
     gene            complement(3902..>5434)
                     /locus_tag="VMZ53_01245"
     CDS             complement(3902..>5434)
                     /locus_tag="VMZ53_01245"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HUS27094.1"
                     /translation="DIGRGSPATRAAGRLFAFVRALGFTGVQLGPQGQTSRSNPSPYD
                     GTIFSRHIGNIAVATLRTLTGDAAVERVLVAGGPARHRHAYDAMHELVGLAYEQLDGR
                     EDLRTRVAAFRVANSAWLESDAAFAAAHDGAPVEHYIFGQFLAHEEHERVRRTCGMLL
                     YGDMQIGYSQADAWRFADAFLPGYLMGAPPSRTNPDGQPWGYPVLDPAQYGGAAAALV
                     VARAEKAFAEYDSIRIDHPHGLVCPWVYRSDSGPHSTAVREGARLFESPDLEDHRRLA
                     AFAIARPEQIDRTKPRYHDGWVTDLDQEQVGRYALLMDAILDAAHRAGRSRDDISCEV
                     LSTMPAPLGAVLRRHGLGRWRVTQKANMDDPNDVYRSESAQREDWVMLGNHDTAPILS
                     VIRGWAPAQREKWARHLAARLRLAQPQRLASDGFMATAMLAELFVSRAENVSIFFADL
                     FGYTERFNVPGLVDEANWSLRLPHDFEQLHAQRLAREEALDLPLAVELALAAQEDGAE
                     RR"
BASE COUNT          976 a         1837 c         1703 g          918 t
ORIGIN      
        1 atccgcagaa gctctatctg ccgccgatca tcgatcccga gtatcactac gaggccgtca
       61 acgtcgaagc gcagcagggc aacccgtcgt cgctgctgtg gtggacgaag cgccttatcg
      121 cgaagcgcaa ggagcacaag ctcttcggcc gcggctcgat cgagttcatc aatggtgaca
      181 acccgcgcgt gctcgcgttc gtgcgcgaat acgagggtga cacggtgctg gtcgtcgcga
      241 acctctcgcg cttcgtgcag tgcgcgcgcc tcaacctcga tcgcttccgc ggcagctcgc
      301 cggtcgagct atttggccgc atgcggttcc ccgaaattcc cgagacgccg tacttcatgt
      361 cgctcgggcc gcacgacttc tactggctac agctcgagaa gccgcaagcg gcagacctgg
      421 ctgtggaccg tccgacgctg tcggcacgcc gagggtggac ggagctgctc gagcctgcgc
      481 ggcgtcgcga cctcgcgaat gcgctgatcc ggtacgcatc gcagcggcgc tggttccgca
      541 gcaaatcgcg gcagcgcaaa gaagcgacga tcacggacat catccggctc gatggtgagc
      601 cgcgcttcgc gatcgcgctg ctgcacgtcg agtacgcgca cggccggccg gagacgtacg
      661 tcgttccgct tgcattcgcc gaggacgcgg aaccacacga gtcgatgcgt tcgcctgggc
      721 tcgtgatcgc caacgtgacg cttagcgatg tgccaagccg cggtcatgtc agcggcgtcc
      781 tctacgacgc actctgtgcc gacacgttcg gcgcggcact gctgcgcgcg atgacgcaag
      841 gcacgggcgg caagggcgag cgcggcacgt tctcgggtag tgcgctcgct gctctgcagg
      901 acctaccggc cgatgcgtcg ctcgttcctc gcctgtcgag cgccgagcag acgaacagca
      961 gcgtcatctt cggcgacaag ctgatgctga agatcttccg cgtgatcgaa gaaggcccga
     1021 gcctcgagta cgagatgggc cacttcctcg catcgcgcga gcctccgtac aaaggcgtgc
     1081 cgcggctcgc cggcgcgttg gaatacgtgg gccacggtcg cgacagcagc acggtcggca
     1141 cactcgccga gctggtcccg aaccaaggcg acgcgtggca gttcacgctc gatgcgctcg
     1201 atcgctactt cgacggcgtg ttgtcgtcgg acaagcgcgc gatgcagccg ccggaccagc
     1261 aaggctcgct catccagcgc gcgcgactgg ccccgagcga cagcgtgctc gatctcgtcg
     1321 gtgcctatat cgatcgcgtg cgcctgctcg gcctgcgcac cgcagagcta cacctcgcgc
     1381 tcgcgagcga tacaagcgac ccgctgttcc gccccgaggc gtacgacatc atgcatcagc
     1441 agtcgatgta cggctcggcc agcgcgcaga tggctcggac gttcgacgtg ctgcgcaaga
     1501 agcgcggaac gctgtctccg gacgctcaca cgatggcgga cgagctgatc gctcacgaga
     1561 gcgacatcga tcgcttgctc gagcgcgtga cgaaacgccg cctcgatgtc gcgcgcatcc
     1621 gcgtgcatgg cgattaccat ctcggccagg tgctgtggac cggcgacgat ttcctcatca
     1681 tcgacttcga aggtgagccg ggtcgtccgc tgtcgcagcg tcggttcaag cgcagtccgc
     1741 tgcgcgacgt cgcgggaatg ctgcgctctc tcgagtatgc gagtgcggca gcgcttcgcg
     1801 atggtcgtca ccgtccagag gacatgccgg ttctctcggt gtgggcgcgc gcgtggacgg
     1861 aatgggtatc ggcgtcgttc ctcggcggct acctcgatcg cgtcgcgggc accaagctca
     1921 tcccgccaaa cgatgcggac accgagcagc tgcttcggtt cttcatgttc gagaaggtga
     1981 tctacgaaat cggctacgag ctcgataacc ggcccgagtg ggtcgagatc ccgatgcgtg
     2041 gtctgctcac gctgctcaag ccatgaagcc ccaattcggc gagctcgacg cgcacctctt
     2101 tcgcgaaggc aagcaccctc gcttgcatga aaagctggga tcgcatcccg acgcgaaggg
     2161 cacgtacttc gccgcatggg caccacacgc cgcagcggtg agcgtgatcg gcgactggaa
     2221 tgcgtggaag ccgaacgtca acgcgctcga gccaatcggt gatacgggcg tgtggcacgg
     2281 ctacgtcaaa ggcgtcgcaa agggcgcgct ctacaagctg cacgttcgct cgaaggtccg
     2341 cggttacgaa gtcgcgaagg ccgatccgtt cgcgctccgt cacgaggaag cgcctggcac
     2401 cgcgtcgatc gtctggacga tggactactc gtggggcgac gccgcatgga tgaagtcgcg
     2461 cgacaacgtc tcggcgacga acgcgcctgt ctcgatctac gaggtgcacc tcggctcgtg
     2521 gatgcgagtc cccgaggaca agaatcgatc gctcacgtac cgcgaaatca cgccgaagct
     2581 cgtcgaggac gtcacgaagc tcggcttcac gcacgtcgag ctgatgccgc tcaccgagca
     2641 tccgttcttc ggctcgtggg gctacgagac gacgggctac ttcgcggcga cgtcgcgcta
     2701 cggaacgccc gaggacctga tggcgctgat cgatgcgctg cacgcggcgg gcgtcgccgt
     2761 gatcctcgac tgggtgcccg ctcacttccc gaccgacgag catggcctcg tttacttcga
     2821 cggcactgcc ctcttcgagc atcccgatcg caagctcggc ttccatcccg agtggaacac
     2881 gtcggtgttc gatttcggcc gccaggaagt ccgcagcttc ctcctgtcga gcgcgctgtt
     2941 ctggctcgag cgctttcacg aagacggcct gcgcgtcgac ggcgtggcat cgatgctcta
     3001 ccgcgactac gcccgcaagc ccggcgagtg gatcccgaac gacaagggcg gcaacgaata
     3061 cttcgaagcc gtcgagctgt tgcagcgtct caacgacgcg atccatcgcg agcaaccggg
     3121 cactgtcacg atcgcggaag agtcgacggc gtggccgaag gtcacacgca atccaagcga
     3181 tggtggcctc ggcttctcct acaaatggga catgggctgg atggccgaca cgctgcgcta
     3241 cttcgcgcgc gatccgatcc atcgccaaca ccatcacaac gagctcacgt tccgtggcgt
     3301 ctacgcattc agcgaacgct tcgtgatgcc gctctctcac gacgaagtcg tccacggcaa
     3361 aggctcgatc ctcgcgaaga tgccgggcga ccgctggcag aagctcgcga acctgcgcct
     3421 gctctacgcc taccaatggt cgcagccggg caagaagctc ctgttcatgg gcgatgaatt
     3481 cgcgcagtgg aacgagtgga accacgactc gagcctcgac tggcacctac tctccgacgc
     3541 agggcatcgc cagatccaac tcctcgtcgg cgagctaaac cgcctctacc gcaccgaacc
     3601 cgcgctgcac gagctcgact gcgagccagc cggcttccaa tggctcatcc ccgacgacgc
     3661 cgtcaacagc gtgctcgcat acgagcgcat ctcgaagggc gatcagcgca tcgtctgcgc
     3721 gctgaacctc acgccggtgc ctcgcttcaa ctatcgcgtg ggcgtcacgc agcccggtca
     3781 gtggagcgag attctcaaca ccgacgcaac ggagctcggt ggcagtggcc acggcaacct
     3841 cggccgtgtc gaagcgatgc cagtccagat gaacggccgc ccgttctcgc tgacgttgac
     3901 gctaccgccg ctcagcgccg tcttcttgcg ccgctaacgc aagctcgacc gcgagtggca
     3961 ggtcgagcgc ttcctcgcgc gcgagccgtt gcgcgtgcag ctgctcgaag tcatgtggaa
     4021 gccgcagtga ccagttcgcc tcgtcgacga gccccggcac attgaagcgc tcggtgtagc
     4081 cgaacaggtc ggcaaagaag atcgagacat tctcggcgcg cgacacgaac agctccgcga
     4141 gcatcgcagt cgccatgaag ccatcgctcg cgagtcgctg tggctgcgcg aggcggagcc
     4201 gcgccgcgag atgccgcgcc cacttctcgc gctgcgcggg tgcccagccg cggatcacgc
     4261 tgaggatcgg cgcggtgtcg tgattgccga gcatgaccca gtcttcgcgt tgcgcgctct
     4321 cgctgcgata gacgtcgttc ggatcgtcca tgttcgcctt ctgcgtgacg cgccaccggc
     4381 caaggccatg ccgtcgcaga acggcgccga gcggtgcggg catggtcgag aggacctcgc
     4441 agctgatgtc gtcgcgcgag cgccccgcgc ggtgagccgc gtcgaggatc gcatccatga
     4501 ggagcgcata ccgccccacc tgttcctgat cgaggtccgt gacccacccg tcgtgatagc
     4561 gaggcttcgt tcggtcgatt tgctcgggtc gcgcgatcgc aaatgccgcg agtcgccgat
     4621 ggtcctcgag gtcaggcgat tcgaacagtc gcgcaccctc gcgcaccgcc gtgctgtgcg
     4681 gcccgctatc gcttcgatac acccacgggc agacgagccc atgcggatga tcgatgcgaa
     4741 tcgagtcgta ctcggcaaac gccttctccg cgcgcgcgac cacgagcgcc gccgccgccc
     4801 ctccgtactg cgcaggatcc agaaccggat atccccacgg ttgcccgtct ggattcgtcc
     4861 tgctcggagg cgcgcccatc aagtatccgg gcaaaaacgc gtcagcaaac ctccacgcat
     4921 cggcctgcga atatccgatc tgcatgtcgc catacagcag catcccgcag gtgcgtcgta
     4981 cgcgctcgtg ctcctcgtgc gcgaggaact ggccaaagat gtaatgctcg accggagcgc
     5041 catcgtgcgc ggcagcgaac gccgcatcgc tttcgagcca cgccgaattc gcaacgcgaa
     5101 acgctgcaac tcgcgttcgc aaatcctcgc ggccatcgag ctgctcatac gcaagcccaa
     5161 caagctcgtg catcgcatcg tacgcgtgac gatgtcgtgc gggcccgcca gcaacgagca
     5221 cacgctccac cgcggcatcc ccagtaagcg tgcgaagggt agcgacagcg atgttgccga
     5281 tgtgccggct gaagatcgtg ccgtcgtatg gcgacggatt acttcgcgac gtctgcccct
     5341 gcgggccgag ctgcacgccc gtgaacccga gcgcacgcac aaacgcaaac aaccggcccg
     5401 ctgcgcgcgt agccggtgat cctctaccga tgtc
//
LOCUS       DATLBZ010000026        16199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6717334, whole genome shotgun sequence.
ACCESSION   DATLBZ010000026 DATLBZ010000000
VERSION     DATLBZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16199
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6717334"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..765)
                     /locus_tag="VMZ53_01250"
     CDS             complement(127..765)
                     /locus_tag="VMZ53_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27095.1"
                     /translation="MGANILTKTDRQMEDRMIAVQGDPLRADTLQKARAFKRTWLELA
                     EALTKVQHGRTWEGWGYPDFDAYCRKELHLRGSTVAKLLGSYSFLEKSAPKVIERARQ
                     DPFEAPIPSMPAVEFVQKARERGAADEETLETIQRVAFEEGADAPALAKQFKDVAFPQ
                     TASDRREKARAAITSTARKLSTLIAEEDSPVPKKLAIRVEELVGELLESLEN"
     gene            936..1676
                     /locus_tag="VMZ53_01255"
     CDS             936..1676
                     /locus_tag="VMZ53_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27096.1"
                     /translation="MRSSLLCLLVLLAGCPGSLKTDALEGGDDSDGNGAGAGNSPTEC
                     RVANDCVAAGPKCCDCPTHAVPKADPTQAACAAVDCAPTQCGSPMEAACNAGRCDLVC
                     SPVQCDANISCAYGLATDGNGCLTCACADAPQSAQCSLDTDCARVKDDCCGCAMGGED
                     TAIPAGQVAAHDAALSCSANPSCPGFNACAPDMQARCVQGECALVTGVIPPNACGRAD
                     LAACGAGESCTVNANDQATMYGVGVCQP"
     gene            1785..3704
                     /locus_tag="VMZ53_01260"
     CDS             1785..3704
                     /locus_tag="VMZ53_01260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27097.1"
                     /translation="MGEVLDGRYRVIKKLGEGGMGEVYAAEHIHIEKRFAVKLLKAEI
                     VSNAEAVKRFQQEARSSSSIKHKNIIGVEDFGRLPDGRIYMCMELLDGQPLNDMIQHP
                     MGADRLLNILIQSGHGLAAAHAKGIVHRDMKPENIYVTNEGPNDVPKLLDFGIAKVAG
                     NEGQNNLTRTGTIFGTPFYMAPEQALGNPVDQRTDIYAMGVIMYECFAGSLPFQGESF
                     MGILTQHITTPPEPVAQRAGKAGRALPMGVAEIITKCMEKDPGARYQTMDELVNALIQ
                     VYRGIAGPGMSTYMEAFPVGSAQHMSQPTPAPMTGAHMVGGNVSGQSRTVGVGTPPPY
                     VPGQSQPMVPQYGSGPSAALSGQSGLYDPSASSSVVVPKKSKTGLIVALVILLAAGGG
                     IAAVVLLGGKKDPGGAGSGSSEVIADNGGSGSAVTPDNGSAVVPNTGSDTTNNGGGTT
                     TNNGSAETPNNGSGAGSSDVANNGSGAMNGSDSGNQAGSDNPLPVVPGTKVRLVTQPY
                     VESFEVFEKGEKVGTEADEFLVPKGETRTIVIKAKGFKDSKPTKIDGKKKSVLIAMTL
                     VKTGGNTGGHTGGNTGGNTGGNTGGNTGGNTGGNTTPPPTGLDCSEKMVDIKNAKCVR
                     QFCAKHPENPGCDTE"
     gene            3774..4760
                     /locus_tag="VMZ53_01265"
     CDS             3774..4760
                     /locus_tag="VMZ53_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS27098.1"
                     /translation="MRVPAPRFLLLLVMALFVAGASARVAHADQRSDAKVHYQAGVKA
                     YSSQDYRTAIKEFSAAQAIMPADLNNYNLALCYDKLGEAEPAIQYYKEYLAKVPNTDK
                     RVEIEASVSRLEAALQSAAAKRAEEQRKADEAAKADAARKADEARKAEEARRAEEAKR
                     QPPIGDAANPDTQTATGVGSTGVPSTGQTVSTGDAQLDRVAGIDVNSIRDQRVGGAGS
                     GMPDNRVGPGGPNGNAGVGAAGGSGGAGMQANAGGAPSGPVGMGPDDKPIKKETPVYK
                     KWWFWAVVAVSAYVVYSIAVDGSSSNTRAREVLPPSSPGADPTSGAAPLFRF"
     gene            4782..5477
                     /locus_tag="VMZ53_01270"
     CDS             4782..5477
                     /locus_tag="VMZ53_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27099.1"
                     /translation="MRWLPLVIVAAGCGRVGFDPLAANADGSLARTCADISNVIACDD
                     FETGLDGWGVPPGIVVVSSPIHGGSGAIEVTIDAGGSANLYVTPAAMAAASQLAARAW
                     YYMPTNAALDHANVMTLQSVSNQLTAFVAGPAGNFNLYANMAGGILSPAGAAIPRDRW
                     FCMELDLVVGPSGSAAMRIDGAPAGTISNVDTRTPGGYTEWLVGVGFSLGTQPAFTFF
                     IDDVVLATTPPGC"
     gene            5595..7469
                     /locus_tag="VMZ53_01275"
     CDS             5595..7469
                     /locus_tag="VMZ53_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27100.1"
                     /translation="MKRAVVLWVSLVVAGCADEQDATSVSTSALTAQQCSYFSVNDKV
                     TICHHTSSSTKPFTVVRTNVASCGGHTAHAGDYVAYDDPTCNGQGCFPAGAPADGTVE
                     CCGNLTAQSGTCGCPPGSSVASDGRSCVQSDQCPADDSKTEPGACGCGVADADSDGDG
                     ALDCFDACPSDAFKVVAGACGCGVSDVDSDGDGAADCHDACPTDALKVVAGACGCGAS
                     DVDSDGDGAADCHDACPTDAAKTEAGTCGCGVADTDSDADGTADCFDACPTNAFKVEA
                     GLCGCEVADDDSDGDGTADCNDACPGDPAKTAPGDCGCDTPEGTCCAGAGAPIGGTAT
                     LVASSNQPQQVAVDASNVYWGSMASGSTPGALWKAPKGGGSAQALFTLGNTNDYVKAS
                     ASDGSNVYFGSSDGTIRKVAATGGAATVVATPGIGTQFFAIDSTNVYFTKSVSGVGGI
                     YRMAKSSGSVVAIEADGVQRCRAGLAIDASFVYWAEDGCFSCSTSTGRIMKAPLAGGA
                     PVVIASGQAHPNFVTVDNDNVYWSNLCDGTIVKVPKAGGASTKLATGQSSPQGIATSA
                     GYLYWTNSGTSPSVMRMSACGGTPTTLFSGGSNLQTLVTDGLRLFWNDFAAGRVMKLP
                     "
     gene            7488..8729
                     /locus_tag="VMZ53_01280"
     CDS             7488..8729
                     /locus_tag="VMZ53_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUS27101.1"
                     /translation="MSITAEDLGSATARLRAQPADFGAWLEVAAVLAALGAADDAEEA
                     FAVIGEGARVHGRVALAVACARHLAERGSVRGPELVDAIVDTYATGGPHFAFTQPPAP
                     DEPPAIGEPPAGEAVARARWTIDQLAPWLVGRQLSSVMPAPLLSALSKPGARALIGVM
                     TARAYATAQTIVEVGEPASALYWLAHGSVAISRPKPEPRAPGSSGPVAQLGELHSGAF
                     FGEIALVGGTTRTANVMATEDCWVLEIPAKAVETAAQKQPKLAEVLAFHARARLLANV
                     TRTSPLFKPLKEADRDELLAKFTSEIVPAGTTFIREGEANDSLWVIVAGWCEVKSGGG
                     AMADVGPGAVVGELSILRGDAASADVTALEPAILLRLAKQDFDAIAKRHPELRTEIGK
                     LLKLREQANRAMFQDATDLIV"
     gene            8726..10171
                     /locus_tag="VMZ53_01285"
     CDS             8726..10171
                     /locus_tag="VMZ53_01285"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HUS27102.1"
                     /translation="MKNAIIALLLVAACTDRGPGPAPKKIEPSYIRENTLKTIPDGIQ
                     RIDANLGGKVLYLGNKTAYLDSRSADAPLAPGGVIRVFHYWQVLVPPGPQFKIFTLVR
                     GAENTVDFMNLPPTDMEIGLPVGKWKSGQIIQDVQDITLRPDWHSPTATLYVGLVEEH
                     GNQIGDRMPIGATDPSMIKDRAIVARTIQVDLSKAPPPAGTVYVPRASSPITVDGVAN
                     EPAWSSAATSTEFATADGSPEPVGKAIGRMTWDDQNLYLFVTVTDTDVFSQYKNHDDP
                     LWKQDCVEMFVDADGNRQGYVELQVNPNNATFDSYFATTRAQPGDEKWDSNMVTAVKA
                     RGTPDKEGDADQGWDVEIAIPLAAVKGRSDTMQVKLPPTVGDRWRLNVVRVDSRSGGG
                     GPSASSWNRISYSDFHALDRMLTVVFADAGGSITATTPVQPVQPALQPAQPALQPAPA
                     ETAAPAIIPAQGSAVMRGSAVAPTPSVPKTP"
     gene            10185..11834
                     /locus_tag="VMZ53_01290"
     CDS             10185..11834
                     /locus_tag="VMZ53_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HUS27103.1"
                     /translation="MKAIALLLLVLAACGPKTTRVGTQPSWRGHSTPTQVIPMTFAPT
                     TDAAVRYNERLEAPPPSPLGDAVLQSVREVAVRLNLPAPAADARLFTACQELAQVVPE
                     EGIIGYSVVEFALQRHGIIEPSPHLLVVWGNIDEPQLIVDQLKPRLPEILGEGTMARV
                     GIGAAKRNADGTGAVVFAMQASGVATNPIPRTLPANGSFKLDAVVDSRYHEPELFITR
                     EDGSTERMGIELGKSGGFKAMVLCGKHIGKQQVEITASDQSGSTVLANFPVWCASEPP
                     QSITIEGTQEDNAVVGAEEAERRLLALMNRDRQAAGLPALLWDERVAQVSRAHSEDMR
                     RTKIVAHISPTTGSAADRVKVAGIKTAVVLENVARAYGVGEAHLGLMNSPGHRANILA
                     AAATHVGVGVILGDDISGRREMFLTQVFIRIPPKVDRAQAADLVRERINAVRPVSVNV
                     KLAGVAQDLAEGLAAGKTRESLWPGAKKKLDGLNTSYARVGSVISAVADLETVDGKQL
                     LGDYKADDIGVGIAQGNHPDIGEGAVWVVVLMAEKLPAPKK"
     gene            complement(11842..13056)
                     /locus_tag="VMZ53_01295"
     CDS             complement(11842..13056)
                     /locus_tag="VMZ53_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27104.1"
                     /translation="MSPTLRILLASTLLAHAAGCATDTSTPPSPDDKDEFRDSDGKTD
                     NGLDVCEAFDWYGDGECDAFCVQRDTDCATDARTPELPPADALASKITMARAAYQVEQ
                     SMGTLIEAKFEHDHSGNLALSLYPLGKSVSVDATNNVFQEAAGDPTATTFAPGLEVFH
                     DFEHLSRSTRDLTIVQLSKLTLRDAIARGSYFGFVYWAIPTMQAGRAGYGIYAVDDDG
                     DSQYVFIDGQGSRVRQVADLGTAPTNPTDDRHPELGNDPTIVRTSKITMLQAIDQLEA
                     QYGKMIEAKFELDHSNNLALSLYPVKDYRLDAERNELGELAGDPTAASFSPSYSKFDV
                     PDEEHVTKASRDLSLVQASGMNLRSAVAKVQAKFPGGFIYWAIPTRKGTRAGYGIYVL
                     DKNNRTHYLFVS"
     gene            complement(13136..14128)
                     /locus_tag="VMZ53_01300"
     CDS             complement(13136..14128)
                     /locus_tag="VMZ53_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27105.1"
                     /translation="MRSVIGLTLLLAACGNDLAPPGTCQVPTGAETPVVDHVQLAGRA
                     ASYDDLRYSPELGKVLAVPLGTARIYMIDPDSLAVTTHGAPGGSESVDASATTIFIID
                     RSNRRVVAIDAESGAMLGAQDTSGYPDYVRFVEKTSELWVTVPGRKRIQIMSAATLAP
                     LANVDVPGAPEGLTISSTGDTAFTQAGGRIIELDVSTRSVTGVWETGCGSSHGFPQID
                     EAFGLAIGGCSSNGGIGVTRMGGDKLAGFEAGGDSAILAYDSARHHLFVRGDPGATLD
                     ILGVCSDGGTSVLGSVELSMNGHGATVDDRGHVWVADADNGAVYRITDPFPATH"
     gene            14279..14878
                     /locus_tag="VMZ53_01305"
     CDS             14279..14878
                     /locus_tag="VMZ53_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27106.1"
                     /translation="MLLLALVSPLAAACTAESVDELELDEEASSKADGAAWYKLYTCN
                     DGQAVLDVNGNERRNLQLVIRDANIIKFFNDRQVVASRYGDTELVLSGWTGYVDFGRA
                     HGPVLRYQPYGGPGIFNRADFDRTELIANANYYYGGPFIRVFRDNAGIKVQAGRIEGR
                     GCARTETYCPGDGFDCQTSCVDEYTEFVESANWYFNNCY"
     gene            14899..15615
                     /locus_tag="VMZ53_01310"
     CDS             14899..15615
                     /locus_tag="VMZ53_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27107.1"
                     /translation="MRRSLLIAAVLLATTATASAGRSHFGWMYGTDIVPERGVEVESW
                     IVEENHKGDSNSGETAFWWGPVMALTQHVELAISVEAAEEDGSPHFTKWGGDIRYRPQ
                     SPDAVDAGPFATKFRLGAKRLILDRAGMRGEADVVASYSAGRVFAQIDAGTIFQHIPD
                     EDEVELRPAAGVSVRAIDDLRFGVEMYSELIVKGEGTSWLTVGPTVSLTSGRFWGAAT
                     YGIGLFGIRDAPRVSFGVAL"
     gene            15612..>16199
                     /locus_tag="VMZ53_01315"
     CDS             15612..>16199
                     /locus_tag="VMZ53_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27108.1"
                     /translation="MKSSALLVACMTASATAYADGSVHGVVEVARPGDVAPSAILVYI
                     VGFEEAAPNKPVEVKQAGKKFIPDLIAVTAGGTVSFPNGDPFLHNVFSPTSERAFDLG
                     SYKQKDTRTRTFPKRGVIDVYCNIHPEMSATLIVLPNTRYAFVDTSGSFRIDHVPPGT
                     WTLFAFSRRAVQPVSQKISVAADGVTEVTLKLDEVK"
BASE COUNT         2863 a         5169 c         5459 g         2708 t
ORIGIN      
        1 tacacgagcc agtcgatcac cgtgccgagc tcgttcaaga atcaatcgat gatggtgcac
       61 gccgacgcgc acgacgtgtc cggcctcgcg tcgaacatca tcgacttcaa ctgcggcttg
      121 cgctgatcag ttctcgagcg attcgagcaa ctcgccgacg agctcttcga cgcgaatcgc
      181 gagcttcttc ggcaccggcg agtcttcctc ggcgatcagc gtcgagagct tgcgggcggt
      241 cgacgtgatc gcggcgcgcg ctttctcgcg gcgatcgctc gccgtctgcg ggaacgcgac
      301 gtccttgaat tgcttcgcga gcgccggtgc gtcggcacct tcttcgaatg cgacgcgctg
      361 gatcgtctcg agcgtctctt cgtcggccgc gccgcgctcg cgcgctttct gcacgaactc
      421 gacagcgggc atcgagggga ttggcgcttc gaatggatct tggcgcgcgc gctcgatcac
      481 tttgggggcg ctcttctcga ggaagctata gctgccgagg agcttggcga ccgtcgagcc
      541 gcgcaggtgc agctctttgc ggcagtaggc gtcgaagtcg gggtagcccc agccttccca
      601 cgtccggccg tgctggacct tcgtcagcgc ttccgcgagc tcgagccacg tgcgtttgaa
      661 ggcacgtgct ttctggaggg tgtccgcgcg gagcggatct ccttggaccg cgatcatgcg
      721 gtcttccatc tgtcgatcag tcttcgtgag gatgttggcg cccatcgctg aagcacccta
      781 gaagatgggt ctgatcacct ctccgcatcg cctgcggcga tgctgcgcgg tccagcagag
      841 ctggaccttg cgatcacctc gaaaaactga ggcgcgacgc ttggatctcg acgggttaga
      901 ctgggcatag gtcctgctga tactcctgtc cgcccatgcg ctcgtccctc ctttgcctcc
      961 tcgtcttgct cgccggttgc cccggctcgc tcaagacaga tgcgctcgag ggcggcgacg
     1021 acagcgatgg caatggcgcc ggtgccggca attcgccgac ggagtgccgc gtcgcgaacg
     1081 actgcgtggc ggcaggcccc aagtgctgcg actgcccgac acacgcggtg cccaaggcgg
     1141 atcccacgca agctgcgtgt gcggcggtgg actgcgcgcc gacgcagtgt ggcagcccga
     1201 tggaagcggc gtgcaatgcg ggtcgctgcg atctcgtgtg ctcgcccgtg caatgcgacg
     1261 cgaacatcag ctgcgcgtac ggcctcgcca ccgacggaaa cggctgcctc acgtgcgcgt
     1321 gtgcagatgc accgcagtcg gcgcagtgct cgctcgatac cgattgcgcg cgcgtgaagg
     1381 acgactgctg cggctgcgcg atgggcggcg aggacaccgc gattcccgcc ggacaggtgg
     1441 cggcccatga cgcggcgctg agctgctcgg cgaatccctc atgcccgggc ttcaacgcct
     1501 gcgctcccga catgcaagct cgctgcgtcc aaggtgaatg cgccttggtg accggcgtga
     1561 tcccgccgaa tgcgtgtggc cgcgcggatc tggcggcttg cggcgcgggt gaatcctgta
     1621 ccgtgaacgc gaatgatcaa gcgacgatgt acggtgtcgg tgtgtgtcag ccttagggct
     1681 gcgcagacca tccgacgggc gtggcgcgtt atactcgtga cctcatgagt gttgaccctc
     1741 ccgcatcggg gtcttttcca caggctccgc ctgcgccgtc attggtggga gaagttctcg
     1801 atggtcgtta ccgcgttatc aagaagctcg gcgaaggtgg catgggcgag gtctacgcgg
     1861 ccgagcacat ccacatcgag aagcggttcg cggtcaaact gctgaaggct gagatcgtct
     1921 cgaacgctga agcggtgaag cgcttccaac aagaggcgcg cagctcgtcg tcgatcaagc
     1981 acaagaacat catcggcgtc gaagactttg gtcggctgcc ggatggtcgc atctacatgt
     2041 gcatggagct gctggatggc cagccgctca acgacatgat ccagcatccg atgggtgcgg
     2101 atcgtctact caacatcctc atccaatcag gtcacggtct cgcagcggcg cacgcgaagg
     2161 gcatcgtcca tcgcgacatg aagccggaga acatctacgt cacgaacgag gggcccaacg
     2221 atgtccccaa gctcctcgac ttcggcatcg ccaaggtcgc gggcaacgaa ggccagaaca
     2281 acctgacacg cacgggcacg atcttcggca cgccgttcta catggcgccc gagcaagcgc
     2341 tcggcaatcc cgtcgatcaa cgcaccgaca tctacgcgat gggcgtgatc atgtacgagt
     2401 gcttcgccgg ctcgctgccg tttcaaggcg agtcgttcat gggcattctc acgcagcaca
     2461 tcacgacgcc gcccgagccg gtcgctcaac gggcaggcaa ggcaggacgc gcgctgccga
     2521 tgggcgttgc cgagatcatc acgaagtgca tggagaagga tccgggcgct cgctaccaga
     2581 cgatggacga gctcgtgaac gcgctcattc aggtctatcg cggcatcgcg ggacccggca
     2641 tgagcacgta catggaagcg tttcccgtgg gaagcgcgca gcacatgtcg caaccgacgc
     2701 cagcgccgat gacgggcgcg cacatggtcg gcggcaatgt cagcgggcag tcgcgcaccg
     2761 tcggtgtcgg cacgccgcct ccgtacgttc ccggtcaatc gcagccgatg gtgccgcagt
     2821 acggctccgg gccgagcgcg gcgctctcgg ggcagtcggg tctctacgat ccgtcagcct
     2881 cgtcgtcggt cgtcgttccg aagaagagca agacgggcct gatcgtcgcc ctggtgatct
     2941 tgctcgcagc gggcggcggc atcgcagcgg tcgtgctcct cggcggtaag aaggaccccg
     3001 gcggcgcggg ctccggctcg agcgaagtca tcgcggacaa cggcggtagt ggcagcgccg
     3061 tgacgcccga caacggcagc gcggtcgtgc cgaacaccgg cagcgacacg acgaacaacg
     3121 gcggcggcac gacgacgaac aacggcagcg ccgaaacgcc gaacaacggc tccggggctg
     3181 gttcgagcga cgtggcaaac aacggcagcg gagcgatgaa tgggagcgac tccggcaacc
     3241 aagccggtag tgacaaccct ctgcccgtcg tccctggtac gaaagttcgt ctagtgacgc
     3301 agccgtacgt cgagagcttc gaggtcttcg agaagggcga gaaggtcggc accgaggccg
     3361 acgagttcct cgtcccgaag ggcgagacgc gcacgatcgt catcaaggcg aagggcttca
     3421 aagattccaa gccgacgaag atcgacggca agaagaagag cgtgctgatc gcgatgacgc
     3481 tcgtgaagac gggcggcaac accggcggtc acacgggcgg caacaccggc ggaaacaccg
     3541 gcggcaacac gggcggcaat accggtggca acaccggagg caacaccacg ccccctccga
     3601 cgggtctcga ctgctcggag aagatggtcg acatcaagaa cgcgaagtgc gttcgtcagt
     3661 tctgtgcgaa gcacccggag aatcccggct gcgataccga gtgactttca acaccacggg
     3721 tggcagccgt caccgcatgt ggcagacctt ctgagccaaa cttcgaggaa accatgcgcg
     3781 taccagctcc ccgcttcttg ctcctgctcg tgatggcgct gttcgtcgcg ggtgccagcg
     3841 cacgcgtcgc tcacgccgat caacgctccg acgcgaaggt tcactaccaa gccggcgtaa
     3901 aggcgtactc gtcgcaggac taccgcaccg cgatcaaaga gttcagcgca gcgcaggcga
     3961 tcatgccggc cgatctgaac aactacaatc tcgcgctctg ctacgacaag ctcggcgagg
     4021 cggagcccgc gatccagtac tacaaggagt acctcgcgaa ggttccgaac acggacaagc
     4081 gcgtcgagat cgaagcgagc gtctcgcgcc tcgaagccgc gctgcaatcc gccgccgcga
     4141 agcgcgccga agaacaacgc aaggcagacg aagccgcgaa ggccgatgct gcacgcaagg
     4201 cggacgaagc acgcaaagcc gaagaggcgc gccgcgcaga agaagcgaag cgccagcctc
     4261 cgatcggcga cgcagcgaat cccgatacgc agaccgcaac gggcgtcggc tcgacgggcg
     4321 tgccgagcac gggccagacc gtgtcgaccg gtgatgcgca gctcgatcgc gtcgcgggca
     4381 tcgatgtgaa ctcgatccgc gatcagcgcg tcggtggcgc cggcagcggc atgccggata
     4441 atcgcgtcgg tcccggcggg cccaacggca acgcgggcgt cggcgcggcc ggcggcagcg
     4501 gcggcgcggg catgcaggcg aacgccggcg gagccccgag tggcccggtc ggcatgggtc
     4561 ccgacgacaa gccgatcaag aaagagacgc cggtctacaa gaagtggtgg ttctgggcgg
     4621 tcgtcgcggt cagcgcatac gtcgtgtact cgatcgcggt cgacggctcg tcgagcaata
     4681 cgcgcgcacg cgaggtgctg ccgccgtcgt cgccgggtgc ggatccgacc tcaggcgctg
     4741 cgccgctgtt ccgcttctga gcgcacgcgt atactttcga cgtgcggtgg ttgccgctcg
     4801 tcatcgtcgc ggccgggtgt gggcgcgtcg gcttcgaccc gcttgcggcg aacgccgatg
     4861 gctcgttggc ccgaacgtgc gccgatattt cgaacgtcat cgcgtgcgac gacttcgaga
     4921 cgggcctcga cggctggggc gtgccgcccg gaatcgtcgt cgtgagctct ccgattcacg
     4981 gtggctccgg tgcgatcgag gtgacgatcg atgcgggcgg gtcggcgaac ctgtacgtaa
     5041 cgccggcggc gatggcggcg gcctcgcagc tcgcggcgcg cgcttggtac tacatgccga
     5101 ccaacgcagc cctcgatcac gcgaacgtga tgacgctgca gtccgtctcg aatcagctga
     5161 ccgccttcgt cgcggggcca gctggcaact tcaacctgta cgcaaatatg gccggtggga
     5221 tcctgtctcc tgcgggcgct gcgattccgc gcgatcgctg gttctgcatg gagctcgatc
     5281 ttgtcgtggg cccgtctggg tcggcggcga tgcgtatcga cggtgcgccc gccgggacga
     5341 tctcgaacgt cgatacgcga acgcccggtg gctacacgga gtggctcgtc ggtgttggct
     5401 tcagcctggg tacgcaaccg gcgttcacgt tcttcatcga cgacgtcgtg ctcgcgacga
     5461 cgccgcctgg ctgctagtcc tcgaatcgtt ggtgctccac gtatcgggta cgtgtcgggt
     5521 gtcatcgcga gtctcgcgtt gacggatcac ggcctggtcc cgtattcagg atgcttcctt
     5581 ctaggagcgt ggctatgaag cgggctgtgg tgttgtgggt gtctttggtg gtcgccggct
     5641 gcgccgacga gcaagacgcg acgtctgtct cgacgagcgc gttgaccgcg cagcagtgca
     5701 gctacttctc ggtgaacgac aaagtcacga tctgtcatca cacgagctcg tcgacaaagc
     5761 cgttcaccgt cgtacgcacg aatgtcgcat cgtgcggcgg ccacaccgcg cacgcgggcg
     5821 actatgtcgc ctacgacgat ccgacgtgca acggccaggg atgcttccct gcgggagcgc
     5881 cggccgacgg cacggtcgag tgttgcggca atctcacggc gcagtccggc acctgcggat
     5941 gcccgccggg ctcgagcgtc gcctcggacg gccgctcgtg cgtgcagagc gatcagtgcc
     6001 cagccgacga cagcaagacc gagcccggag catgtggctg cggtgtcgcc gacgccgaca
     6061 gcgacggtga tggcgcgctc gattgcttcg acgcgtgtcc aagcgatgcg ttcaaggtcg
     6121 ttgcaggcgc gtgcggctgc ggcgtctcgg atgtcgatag cgatggcgat ggcgccgccg
     6181 attgtcacga tgcgtgtccg accgatgcgc tcaaggtcgt tgcgggcgca tgcggctgcg
     6241 gcgcgtcgga tgtcgatagc gatggcgacg gcgccgccga ttgtcacgat gcgtgtccaa
     6301 ccgatgcggc gaaaacggaa gccgggacgt gcggctgcgg cgtcgcggac accgacagcg
     6361 atgccgatgg caccgctgac tgcttcgatg cgtgcccgac caatgcattc aaagtcgagg
     6421 ccggcctttg cggttgcgaa gtcgccgatg acgacagcga tggcgacggc acggccgact
     6481 gtaacgatgc gtgtccgggc gatcctgcga agacggcgcc cggcgactgc ggatgcgata
     6541 cgccggaggg cacctgctgc gccggcgcgg gtgcgccgat cggaggcacg gcaacgctcg
     6601 tcgcgtcgtc gaatcaaccg cagcaagtcg cggtcgatgc gagcaacgtg tattggggct
     6661 cgatggcttc tggatcgacg ccaggcgcgc tgtggaaggc gccgaagggc ggcggctctg
     6721 cgcaggcgct cttcacgctc ggcaatacga acgattacgt gaaggcgagc gcctcagacg
     6781 gctccaacgt gtacttcggc tcgagtgacg ggacgattcg caaggtggcg gcgacgggtg
     6841 gagccgcgac ggtcgtcgcg acgcccggca tcggcacgca gttcttcgcg atcgacagca
     6901 cgaacgttta cttcacgaag tcggtctcgg gcgtcggcgg catttaccgc atggcgaagt
     6961 cgtctggctc ggtggtcgcg atcgaagcgg atggtgtcca gcgctgtcga gcgggcctcg
     7021 ccatcgacgc gagctttgtg tactgggccg aggacggctg tttttcgtgc agcaccagta
     7081 cgggccgcat catgaaggcg ccgctcgctg gtggcgcgcc cgtcgtgatc gcgagcggtc
     7141 aggcgcatcc gaacttcgtc accgtcgaca acgacaatgt gtactggtcg aacttgtgcg
     7201 acggcacgat cgtcaaggtg ccgaaggcag gtggtgcgtc gacgaaactg gcgaccggac
     7261 agtcgtcgcc gcaggggatc gcgacgagcg cgggttacct gtactggacg aattcgggca
     7321 cgagtccgag cgtcatgcgg atgtctgcgt gcggcggaac gccgacgacg ctgttctccg
     7381 gcggcagcaa tctccagacg ctcgtcaccg acggactgcg tctgttctgg aacgacttcg
     7441 ccgcgggccg tgtgatgaag cttccctgat cgcggtatac agatgacgtg agcatcaccg
     7501 ccgaggatct cgggtcagcg acggcgcggc tgcgcgctca gcccgccgat ttcggcgcat
     7561 ggctcgaggt cgcggcagtg ctcgcggcac tcggtgcggc ggacgatgcg gaagaggcgt
     7621 tcgcggtcat cggcgaggga gcacgcgtgc atgggcgcgt cgcgcttgct gtcgcgtgtg
     7681 cgcggcatct cgccgagcga ggcagcgtgc gcgggccgga gctcgtcgat gcgatcgtcg
     7741 atacgtatgc gaccggcggc ccgcacttcg cgttcacgca gcctcctgcg cccgatgagc
     7801 cgcccgcgat tggtgagccg ccggcgggag aggcggtcgc gcgggcgcgg tggacgatcg
     7861 accagctcgc gccgtggctc gtcgggcggc agctgtcgag cgtgatgcct gcgccgcttt
     7921 tgtccgcgct gtcgaagccc ggcgcccgtg cgctgatcgg cgtgatgacc gcgcgcgcgt
     7981 acgcgacggc gcagactatc gtcgaggtcg gcgagccggc gagcgcgctg tattggctcg
     8041 cgcatggcag cgtcgcgatc tcgcggccga agcccgagcc gcgtgcgcca ggttcgtcgg
     8101 gcccggtcgc gcaactcggc gagctgcaca gcggtgcgtt cttcggcgag atcgcgctcg
     8161 tcggtggaac gacgcggacc gcgaacgtga tggcgaccga agactgctgg gtgctcgaga
     8221 taccggcgaa ggccgtcgag accgccgcgc agaagcagcc aaagctcgcc gaggtgctcg
     8281 catttcatgc gcgcgcgcga ctgctcgcga acgtcacgcg cacgtcgccg ctgttcaagc
     8341 cgctgaagga agcggatcgc gacgagctgc tcgcgaagtt cacgagcgag atcgtgccgg
     8401 cgggcaccac gttcattcgc gaaggcgagg cgaacgattc gctgtgggtg atcgttgcgg
     8461 ggtggtgcga ggtgaagagc ggcggtggcg cgatggccga tgtcgggccg ggcgccgtcg
     8521 tcggtgagct cagcatcttg cgcggtgatg cggcgagcgc ggacgtgacg gcgctcgagc
     8581 ccgcgatcct gttgcgtttg gcgaagcagg acttcgacgc gatcgcgaag cgtcatcccg
     8641 agctgcgcac tgaaatcggc aagctcttga agctgcgcga gcaggcaaat cgcgcgatgt
     8701 tccaggacgc cacggatctc atcgtatgaa gaacgcgatc attgctcttc tgctcgtcgc
     8761 cgcgtgcacc gatcgcggac cgggacccgc gccaaaaaag atcgagccgt cgtacatccg
     8821 cgagaacacg ctcaagacga ttcccgacgg gattcagcgc atcgacgcga acctcggcgg
     8881 caaggtcctg tacctcggca acaagaccgc atacctcgat agccgcagtg ccgatgcgcc
     8941 gctcgcgccc ggtggcgtga ttcgcgtgtt ccattactgg caggtgctcg tgccgcccgg
     9001 cccgcagttc aagatcttca cgctcgtgcg cggtgccgag aacacggtcg acttcatgaa
     9061 cctcccgccg acggacatgg agatcgggct gcccgtcgga aaatggaagt cgggccagat
     9121 catccaggac gttcaagaca tcacgctgcg cccggactgg cactcgccga ccgcgacgct
     9181 gtacgtcggc ctcgtcgagg aacacggcaa tcagatcggc gatcgcatgc cgatcggcgc
     9241 gaccgacccg tcaatgatca aagaccgcgc gatcgtcgcg cgcacgatcc aagtcgatct
     9301 atcgaaggcg ccgccgcctg caggcaccgt gtacgtcccg cgcgcgtcgt cgccgatcac
     9361 ggtcgatggt gtcgcgaacg agcccgcgtg gagcagtgca gcaacgtcga cggaattcgc
     9421 gaccgccgac ggcagccccg agcccgttgg caaagcgatc gggcgcatga cgtgggacga
     9481 ccagaacctc tatctgttcg tcacggtcac cgacaccgac gtgttcagcc agtacaagaa
     9541 ccacgacgat ccgttgtgga agcaggactg cgtcgagatg ttcgtggacg ccgacggcaa
     9601 tcggcagggc tacgtcgagc tgcaggtcaa tccgaacaac gcgacgttcg attcgtactt
     9661 cgcgaccacg cgcgcgcaac cgggcgacga gaagtgggac tcgaacatgg tcaccgccgt
     9721 caaagcgcgc ggcacaccag acaaggaagg cgacgccgac caaggctggg acgtcgagat
     9781 cgcgattccg ctcgcggctg tgaagggcag gagcgacacg atgcaggtga agctgccgcc
     9841 aacggtcggc gatcgctggc gcctcaacgt cgtgcgcgtc gattcgcgca gcggcggcgg
     9901 cggaccgagc gcgtcgagct ggaatcggat cagctactcg gactttcacg cactcgatcg
     9961 catgttgacc gtcgtgttcg ccgacgccgg cggatcgatc accgcaacga cgccggtaca
    10021 gcctgtgcag cccgcgctgc aacccgcgca gccagcgctt cagcctgcgc ctgcggagac
    10081 agcagcgcct gcgattattc cggcgcaggg ctccgctgtg atgcgcggct cagccgtcgc
    10141 gccgacgccg agcgttccga aaaccccgtg ataggctgag cggagtgaag gcgatcgcgc
    10201 tgctcctcct cgtgctcgcc gcgtgcgggc cgaagacgac gcgcgtaggc acgcagccga
    10261 gctggcgcgg ccacagcacg ccgacgcagg tcatcccgat gacgttcgcg ccgaccaccg
    10321 acgcggcggt tcgctacaac gagcgcctcg aagcgccgcc gccatcgccg ctcggtgatg
    10381 ccgtgctcca atccgttcgc gaagtcgcgg tgcgcctcaa ccttccggca cctgcggcgg
    10441 atgcgcgctt gttcaccgca tgccaagagc tcgcgcaggt cgtgcccgag gaaggcatca
    10501 tcggttacag cgtcgtcgag ttcgcgctac agcgtcacgg cattatcgag ccgtcgccgc
    10561 acctgctcgt cgtgtggggc aacatcgacg agccgcagct gatcgtcgat cagctcaagc
    10621 cgcggctccc cgagatcctc ggcgagggca cgatggcgcg cgtcggcatc ggcgcggcga
    10681 agcgcaacgc agatggcacc ggtgcggtcg tgttcgcgat gcaggcgagc ggcgtcgcga
    10741 ccaacccgat cccgcgcacg ctccccgcaa acggctcgtt caagctcgac gctgtcgtcg
    10801 acagtcgcta tcacgagccc gagctgttca tcacgcgcga ggacggctcg acggaacgca
    10861 tgggcatcga gctcggcaag tccggcggtt tcaaggcgat ggtcttgtgc ggcaagcaca
    10921 tcggcaaaca gcaggtcgag atcactgcga gcgatcaatc gggctcgacc gtgctcgcca
    10981 acttcccggt gtggtgcgcc agcgagccgc ctcagtcgat cacgatcgaa ggaacgcaag
    11041 aagacaatgc ggtcgtcggt gcggaggaag ccgagagacg cctgctcgca ctgatgaatc
    11101 gtgaccggca agccgcgggc ctccccgcgc tgctctggga cgaacgcgtc gcgcaggtct
    11161 cgcgcgctca ctccgaagac atgcgccgaa cgaagatcgt cgcgcacatc tcgccgacga
    11221 ccggctcggc cgccgatcgc gtgaaggtcg ccggcatcaa gaccgccgtc gtgctcgaga
    11281 acgttgcgcg cgcgtacggc gttggtgaag cgcatctcgg cctcatgaac agccccggcc
    11341 atcgcgcaaa catcctcgcc gcggcagcga cgcacgtcgg cgtgggcgtg atcctcggcg
    11401 acgacatctc gggccgccgc gaaatgtttc tgacgcaggt cttcatccgc atcccgccga
    11461 aagtcgatcg cgcgcaagcc gccgacctcg tacgcgagcg catcaacgcg gtgcgcccgg
    11521 tctccgtcaa cgtcaagctc gcgggcgtcg cgcaggatct cgccgaaggc ctcgccgcgg
    11581 gcaagacgcg cgaatcactg tggccgggcg cgaagaagaa gctcgacggt ctgaacacat
    11641 cgtatgcgcg cgtcggcagc gtgatcagcg cggtcgccga tctcgagacc gtcgacggca
    11701 agcagctcct cggcgactac aaagccgatg acatcggcgt tggcatcgcg cagggcaacc
    11761 atcccgacat cggcgaaggc gccgtgtggg tcgtcgttct gatggccgag aagctgcccg
    11821 ccccgaagaa gtagtgcgcg ctcagctcac gaacaggtag tgcgtgcggt tgttcttgtc
    11881 gagaacgtag atgccgtagc cggcacgtgt gccctttcgc gtcggaatcg cccagtagat
    11941 gaagccaccg gggaacttcg cctggacctt cgcgacggcg gaacgcaagt tcatcccgct
    12001 cgcctgcacg agagacagat cgcgcgaggc cttcgtcacg tgctcttcat ccggcacgtc
    12061 gaacttcgag tacgacggcg agaacgacgc agcggtcggg tcgcccgcca gctcgccgag
    12121 ctcgttgcgc tccgcatcga ggcggtagtc cttcaccgga tagagcgaga gcgcgaggtt
    12181 gttcgagtga tcgagctcga acttcgcctc gatcatcttg ccgtactgtg cttcgagctg
    12241 atcgatcgct tgcagcatcg tgatcttcga cgtgcgaacg atcgtcggat cgttgccgag
    12301 ctcgggatgg cgatcgtcgg tcggattcgt cggcgccgtg ccgaggtccg ccacttggcg
    12361 aacgcggctg ccctggccgt cgatgaagac gtactggctg tcaccgtcgt cgtcgaccgc
    12421 atagatgccg tagcccgcgc gaccggcctg catcgtcggg attgcccagt agacgaagcc
    12481 gaagtagctg ccgcgcgcga tcgcatcgcg aagcgtgagc ttcgagagct ggacgatcgt
    12541 caggtcgcgc gtcgagcggc tgaggtgctc gaagtcgtgg aacacttcga ggcccggagc
    12601 aaacgtcgtc gccgtcggat ctccggcggc ttcctggaag acgttgttcg tcgcgtcgac
    12661 ggagaccgac ttgccgagcg ggtagagcga gagcgcgagg ttgcccgagt gatcgtgctc
    12721 gaactttgct tcgatgagcg tgcccatcga ctgttcgact tgatacgcgg cgcgggccat
    12781 cgtgatcttc gacgcgagcg cgtcggccgg cggaagctct ggcgtgcgtg catcggtggc
    12841 gcagtcggtg tcgcgctgca cgcagaacgc gtcgcactcg ccatcgccgt accaatcgaa
    12901 cgcctcgcag acgtcgaggc cattgtcagt cttgccatcg ctgtcgcgga attcgtcttt
    12961 gtcgtccggc gacgggggcg tggaggtgtc cgtcgcgcag ccggcggcgt gtgcgagcag
    13021 agtcgaggca aggaggattc ggagagtcgg ggacattgca ggctccagtt tcttggttgg
    13081 tgcccgcagg aggaacgagg tcgatcgtgt cgcacaaaaa tcgacatcgg ccacatcagt
    13141 gcgtcgctgg gaacggatcg gtgatgcgat agaccgcgcc gttgtcggcg tccgcaaccc
    13201 acacgtgacc gcgatcgtcg acggtagcgc cgtgcccgtt catcgatagc tccacgctcc
    13261 ccagaacgct cgttcctccg tcgctgcaaa cgccgaggat gtcgagcgtc gcgcccgggt
    13321 cgccgcggac gaatagatga tggcgcgcgc tgtcatacgc gaggatcgcg gagtcaccgc
    13381 cagcctcgaa gcctgcgagc ttgtcgccgc ccatcctcgt gacgccaatg ccgccgttgc
    13441 tcgagcaacc accgatcgcg agaccgaatg cttcgtcgat ctgaggaaag ccgtgcgacg
    13501 acccgcagcc cgtctcccat acaccggtca cggagcgtgt cgacacgtcg agctcgatga
    13561 tgcgaccacc cgcttgcgtg aatgccgtgt cgccggtcga gctgatcgtc agaccttcgg
    13621 gcgcaccggg gacgtcaaca ttcgcgagcg gcgcgagcgt ggccgcactc atgatctgga
    13681 tgcgtttacg tccgggaaca gtgacccaca gctcggacgt tttctcgacg aaccgtacat
    13741 agtccggata gcccgacgtg tcttgcgcac cgagcatcgc gccggactcg gcatcgatcg
    13801 cgacaacgcg acggttcgag cggtcgatga tgaagattgt cgtcgcactc gcatccaccg
    13861 attcggagcc gcccggcgcg ccgtgcgtcg tgaccgccaa cgaatcggga tcgatcatgt
    13921 agatccgcgc agtgccgagc ggtacggcga gcaccttgcc gagctccggc gaatagcgaa
    13981 gatcgtcgta actcgcggcg cggccggcga gctgcacgtg atcgacgaca ggcgtctcgg
    14041 cgccggtagg cacctgacac gtgcccggcg gtgccaggtc gtttccacac gcggcgagga
    14101 ggagcgtcag accgataacg gaacgcatgc cgttggagga gcgagcggac gaaagtcgca
    14161 ccggaatcgc agctccttcg cgattgcacg tacttgcgca gagcaggtgc gaccgactcg
    14221 cgctcgctcc tcctgggagc atcaacacga accctggaga cgatcgatgc gtaccctgat
    14281 gctgcttctt gccctggttt ccccacttgc cgccgcgtgc accgccgaat ccgtcgacga
    14341 gctcgagctc gacgaggagg cgtcttcgaa ggccgacggc gccgcctggt acaagctgta
    14401 cacctgcaac gacggtcagg cggtgctcga cgtgaatggc aacgagcgcc gcaacctcca
    14461 gctcgtcatt cgcgacgcga acatcatcaa gttcttcaac gaccgccaag tcgtcgcgtc
    14521 acgctatggg gacaccgagc tcgtgctgtc cggctggact ggctacgtcg acttcggccg
    14581 cgctcacggt ccggtcctgc gctaccagcc gtatggcggg ccaggcatct tcaaccgtgc
    14641 tgacttcgat cgcaccgagc tcatcgcgaa cgcgaactac tactacggtg gtccgttcat
    14701 ccgcgtgttc cgcgacaacg caggcatcaa ggtgcaggcg ggacgcatcg agggtcgcgg
    14761 ctgtgcgcgc accgagacgt actgccctgg tgacggcttc gactgccaga cgagctgcgt
    14821 cgacgagtac accgagtttg tcgagagcgc gaactggtac ttcaacaact gctactagag
    14881 tgctgttata gacatttcat gcgtcgctcg ctcctcatcg cagctgttct cctcgcgacg
    14941 acggccaccg cgagcgcagg acgttcgcac ttcggctgga tgtatgggac cgacatcgtt
    15001 cccgagcgcg gtgtcgaagt ggagagctgg atcgtcgagg agaatcacaa gggcgattcg
    15061 aacagcggtg agaccgcatt ttggtgggga ccggtcatgg ccctcacgca gcacgtcgag
    15121 ctcgcgatct cggtcgaggc tgcggaggaa gatggatcac cacacttcac gaagtggggt
    15181 ggcgacattc gctatcgccc acagtcgccc gatgcggtcg acgcaggacc cttcgcgacg
    15241 aagttccggc tcggagcgaa gcggctgatt ctcgaccgcg ctggcatgcg tggcgaggca
    15301 gacgtcgtcg cgagctattc ggcgggccgc gtgttcgcgc agatcgacgc aggcacgatc
    15361 tttcaacaca tccccgacga ggacgaggtc gagctgcgcc ccgccgccgg cgtcagcgtg
    15421 cgtgcgatcg atgatcttcg gttcggcgtc gagatgtaca gcgagctgat cgtgaagggc
    15481 gagggcacga gctggctcac cgtgggccct acggtatcgc tcacgagcgg tcggttctgg
    15541 ggcgcggcga cctacggcat cggtctgttc gggattcgcg acgcgcctcg cgtgagcttc
    15601 ggagtcgctc tgtgaagagc agcgcgctgc tcgtcgcgtg catgacggcg agcgcgacgg
    15661 cgtacgccga tggcagcgtg cacggcgttg tcgaagtagc gcgtcctggg gacgtcgcgc
    15721 cgagcgccat cctcgtctac atcgtcggtt tcgaggaggc ggcgccaaac aagcccgtcg
    15781 aggtgaagca ggcgggcaag aagttcattc ccgacctcat cgccgtgact gcaggcggca
    15841 ctgtgtcatt tcctaatggc gatccgtttc tgcataacgt gttctcgccg acgagcgagc
    15901 gcgcattcga tctcggcagc tacaagcaga aggacacgcg cacgcgaacg tttcccaagc
    15961 gcggcgtcat cgacgtgtac tgcaacatcc atcccgagat gtcagcgacg ctgatcgtgt
    16021 taccgaacac gcggtatgcg tttgtcgata cgagcggaag cttccggatc gatcacgtac
    16081 cgccgggcac gtggacgctg ttcgcgttct cacgtcgtgc tgtgcagccg gtcagccaga
    16141 agatcagcgt cgcagccgac ggtgtcaccg aggtcacgct gaagctcgac gaggtgaag
//
LOCUS       DATLBZ010000027         2913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3747312, whole genome shotgun sequence.
ACCESSION   DATLBZ010000027 DATLBZ010000000
VERSION     DATLBZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3747312"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..976
                     /locus_tag="VMZ53_01320"
     CDS             86..976
                     /locus_tag="VMZ53_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27109.1"
                     /translation="MKRQLIIAFAVLSFTASSAFAQLSKDACVDAHSRGQDAREQGKI
                     SLARKLFLTCAQNGCPAAVQGDCSRFADDLSGMQPTVVFVARDGNGNDLPETTVYVDG
                     ALVLTTLDGKPHDIDPGNHVVKFSNGGKDEVVTVVIGSGEKGRTVSAKFGSATVVTPT
                     TTASTTTTAETKPMKPAAPKTTHPKAAMPIAIGGAVLTAVGAGLFVWGRSQVPDQCDI
                     GTHQCAAPPGDPVFKEASDAAGKSNMGMIVGGIGAAAVVGGVVWYFAGSKTEKETSTQ
                     QAIAPYVTGDGAGVSVMGRF"
     gene            complement(1036..1416)
                     /locus_tag="VMZ53_01325"
     CDS             complement(1036..1416)
                     /locus_tag="VMZ53_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HUS27110.1"
                     /translation="MQLHRDIGKIQGAGAELYVIGNGTPSFIEGFREQTKYDGPLYTD
                     PSLEVYKAAELKRGVTKVLNIKAALPTLKAFAKGGKQGLTQGDAWQQGGVVVVASDGS
                     VKWQHASGYPGDNASVEQIVRALR"
     gene            complement(1438..1563)
                     /locus_tag="VMZ53_01330"
     CDS             complement(1438..1563)
                     /locus_tag="VMZ53_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27111.1"
                     /translation="MLTRADDLAKMTVLDEKGGKVELGTLWRDKAAVLAFVRHFG"
     gene            1586..1990
                     /locus_tag="VMZ53_01335"
     CDS             1586..1990
                     /locus_tag="VMZ53_01335"
                     /inference="COORDINATES: protein motif:HMM:NF036804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HUS27112.1"
                     /translation="MAKDACPCGSREPSAACCALYHDGVPAPTALALMRSRYTAFVRG
                     EIDYLIETHDEATRAAVDKKALAQWSKDTLWMGLEIVATEAGGEEDETGIVEFIARGV
                     TNGQPFAQRERSRFRRAFGRWVYTDGNVRRSS"
     gene            2000..2215
                     /locus_tag="VMZ53_01340"
     CDS             2000..2215
                     /locus_tag="VMZ53_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27113.1"
                     /translation="MPNNVLSELSPYGWGASDCWKMWSIVLDILRRYEQTGIPQPELP
                     RSIQRPGALIGLSIGLAVRQAALPRSA"
     gene            complement(2202..>2913)
                     /locus_tag="VMZ53_01345"
     CDS             complement(2202..>2913)
                     /locus_tag="VMZ53_01345"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HUS27114.1"
                     /translation="HSSDPADYVAGFPQHPHRGFETVSYVLRGGFQHRDSVGNSGLIA
                     DGGAQWMTAGHGIVHEEMPRATTTSELWGLQLWVNLPAARKLIAPRYQDLGGAQVPEL
                     DVADARVRLVAGQVGSARGPVDGIDIRPTMLDATLLPRAEFRHSLPGGDTAFVYVLDG
                     VVQLGQLGTRVSAGELAVLGSGSTIVAASDTGGRFLLVSGTPLNEPIARRGPFVMNTD
                     AELQQAFDDYRSGRLTQI"
BASE COUNT          471 a          970 c         1003 g          469 t
ORIGIN      
        1 gagaagccgg cgaacgactg cagcccgccc tactactacg aaggccagaa gaaggtcttc
       61 aaaccgaact gcctctagga gacacatgaa gcgtcaactc atcatcgcgt tcgcggtcct
      121 cagcttcaca gcgtcgagcg cgttcgcgca gctctccaaa gacgcttgcg tcgacgcgca
      181 cagccgcggt caggatgcgc gcgagcaggg caagatctcg ctcgctcgca agctgttcct
      241 gacgtgtgcg cagaacggct gtcccgccgc cgtgcagggc gactgctcgc gcttcgccga
      301 cgacctctcg ggcatgcagc cgacggtcgt gttcgtcgcg cgcgacggca acggcaatga
      361 cctgcccgag accacggtct acgtcgacgg cgccctcgtg ctcaccacgc tcgacggcaa
      421 gccgcacgac atcgacccgg gcaaccacgt cgtgaagttc tcgaacggcg gcaaggacga
      481 agtcgtcacc gtcgtgatcg gctccggcga aaagggccgc acggtctcgg cgaagttcgg
      541 ctcggcgacg gtggtaacgc cgacgaccac cgcatcgacg acgacgaccg cggagacgaa
      601 gccgatgaag ccggcggcgc cgaagacgac gcatccgaaa gctgcgatgc cgatcgcgat
      661 tggtggcgcg gtgctgactg cggtcggcgc gggcctcttc gtgtggggcc gctcgcaggt
      721 ccccgaccaa tgcgacatcg gcacgcacca gtgcgccgct cctccaggag accccgtctt
      781 caaggaggcc tcggacgcgg cgggcaagtc gaacatgggc atgatcgtcg gtggcatcgg
      841 tgccgcggcg gtcgtcggtg gcgtcgtctg gtacttcgca ggcagcaaga cggagaagga
      901 aacctcgacg cagcaagcga tcgcgccgta cgtgacgggc gatggcgcag gcgtctcggt
      961 gatgggccgc ttctaagcgc tgctctgtga ttcgcgcgtg gggctccgag caatcggggc
     1021 ccttcgcatt ttcggctagc gcagcgcgcg cacgatctgc tcgacgctcg cgttgtcgcc
     1081 cgggtagccg ctcgcgtgtt gccacttcac gctgccgtcg ctcgcgacga cgacgacgcc
     1141 gccctgttgc cacgcgtcgc cctgcgtcag cccttgcttg ccgcccttgg cgaaggcctt
     1201 gagcgtcggc agcgcggcct tgatgttgag caccttggtg acgccgcgct tcagctcggc
     1261 cgccttgtag acctcgagcg atgggtccgt gtagagcggg ccgtcgtact tcgtctgctc
     1321 gcgaaagccc tcgatgaagc tcggcgtgcc attgccgatc acgtagagct cggcgccggc
     1381 gccctggatc ttgccgatgt cgcgatgcaa ctgcatcacg tgctcgcggc agtggattca
     1441 accgaagtgg cggacgaacg cgaggacagc ggccttgtcg cgccagagtg tcccgagctc
     1501 gaccttgccg cccttctcgt cgagcacggt catcttggcg aggtcatccg cgcgcgtcag
     1561 catgcggcta tcgtaacgta gacgcgtggc gaaggatgca tgtccctgcg gctcgcgcga
     1621 gccatccgcc gcgtgctgcg cgctctatca cgatggcgtg cccgcgccga cggcactcgc
     1681 gctcatgcgg tcgcgctaca cggcgttcgt gcgcggcgag atcgactatc tgatcgagac
     1741 gcacgacgag gcgacgcgcg ctgcggtcga caagaaggcg ctcgcgcagt ggtcgaagga
     1801 cacgctgtgg atgggcctcg agatcgtcgc gaccgaggca ggcggcgagg aggacgagac
     1861 cggcatcgtc gagttcatcg cgcgcggcgt gacgaacggc cagcccttcg cgcagcgcga
     1921 gcggtcgcgg tttcggcgcg cctttgggcg ctgggtctat acggacggga atgtcagacg
     1981 gtcgtcttag atagtggatg tgccgaataa cgtcctctct gagctgtcgc cgtacggttg
     2041 gggcgcgagc gactgctgga agatgtggag catcgtgctc gacatcctgc gccgctacga
     2101 gcagaccggg atcccgcagc ccgagctgcc tcgatcgatc cagcgtcccg gcgcgctaat
     2161 cggtctttcg atcgggctcg ccgttcggca agccgcgctg cctagatctg cgtgagccgg
     2221 ccgctgcggt agtcgtcgaa cgcttgctgc agctcggcgt cggtgttcat cacgaagggg
     2281 ccgcgccgcg cgatcggctc gttcagcggc gtgccggata cgaggaggaa gcggccgccg
     2341 gtatcgctcg ccgcgacgat cgtcgagccc gagccgagga ccgcgagctc gcccgccgag
     2401 acgcgcgtgc cgagctgccc gagctggacg acgccgtcga gcacgtagac gaacgcggta
     2461 tcgccacccg gaagtgagtg ccgaaactcc gcgcgaggca gcagcgtggc gtcgagcatc
     2521 gtcgggcgga tgtcgatgcc gtcgacgggg ccgcgcgcgc tgccgacctg tccagcgacc
     2581 agacgcacgc gagcatccgc gacgtcgagc tcggggacct gcgcaccgcc gagatcctgg
     2641 tagcgcggtg cgatcagctt gcgggcggcg ggcaggttca cccacagctg taggccccac
     2701 agctcggatg tggtcgtcgc acgcggcatc tcctcgtgca cgatgccgtg gccagccgtc
     2761 atccactgcg ccccgccatc ggcgatgagc ccgctgttgc cgacgctgtc gcggtgctgg
     2821 aagccgcccc tcagcacgta ggagaccgtc tcgaagccgc gatgcggatg ttgcggaaag
     2881 cccgccacat aatcggccgg gtcggagctg tgc
//
LOCUS       DATLBZ010000028        10849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7463758, whole genome shotgun sequence.
ACCESSION   DATLBZ010000028 DATLBZ010000000
VERSION     DATLBZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7463758"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VMZ53_01350"
     CDS             <1..1257
                     /locus_tag="VMZ53_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HUS27115.1"
                     /translation="QPDRQPEMGSLSDAAVLPRLIYGFAPSWQKVLEPVAGRYLDKAV
                     PNPLPEAIKAEADRVVAGAKDEAEKAQKLYAFVAHDIRSIDLPLGAAGYEPHAPEVVL
                     QNRYADDRDKVGLLLAMASSQGIRGRAVLVRTGKVPVISSVPTLAQFDRMIAKLDVGG
                     KDTWLDPSDENGQYAVAFAGQDNLVLPLDKGGVELGSRPALDPSTSVSSTKATYTLSA
                     NGDLSAKYTYELSGWYADRAQGELRPLKGELADKFFQQEAAGVAASALDKGHTVSDTQ
                     SVTGAVTVTQDVSVPGYSSAQANMRVFELPPVAFSVADDEPSASLSTRKTPLWVGVPR
                     TERGEITVQIPAGWKVAYVPPKLEGSAEGVKFSSECSAAGQTVTCKGEMVLDKLVVPA
                     DKYGAFREALTKLQAYERRVVLLQKG"
     gene            1254..1985
                     /locus_tag="VMZ53_01355"
     CDS             1254..1985
                     /locus_tag="VMZ53_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS27116.1"
                     /translation="MRRLALISIVLVGCGRISFEPHTAHDDGAIGDVVLDQPGDATVD
                     PDLLFYVSFDGGLADDARGHALSCMGTCDSIPGRVGPNAVHLNGASCIVLPDAGDLHP
                     ATYTVSVWARLPVPAANSDLVAHPLNGATSFIDTFELWVQPDSGVVMLANSVQVLTTG
                     LDVTTWHHYVAVYDGSAVLSYVDGGLAGGSSGTTTTMYGADPYLIGCDRDNGTEIAHL
                     TGDVDDVRFYGRALTANEIVALANL"
     gene            complement(1982..4552)
                     /locus_tag="VMZ53_01360"
     CDS             complement(1982..4552)
                     /locus_tag="VMZ53_01360"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HUS27117.1"
                     /translation="MNRVAFVSFIALLMHGCGDDAATCALPFADGDPTGHAEPLGAGP
                     GEARAGRIHGEQLAPPGVGVPQLLTWADNDFVIANNRVALVIEDVGDSDLYDPWGGRP
                     VGLARVENGHIAEPSNFGEFFILTGRSTVVTESVSVINDGSHGGTAIIRARGKLHPLP
                     FFEPLTAILFNDEYTDIEAAIDYELAPDADHVDIRVRYHSPRSMETKVPSTMHGLMYS
                     GRTSPTVFQPNVGFDSQLSSTPYVAFVEDNATSWAYMPGEGTLGSSIAASGFLGAFGP
                     GYTIPPCGSEFDRLAVKLIVGGPGLDPVQTAAAKLTGENREREITGTVQRGGQNLLGV
                     HVHAIDAAGHYLSRATTQGARGGFTLHVPAGADITLRAYTRETGLVDVPATSDSMTIT
                     LPPVGYVNVVATENGAAVPARIQVLSTAAPPPANYGEPDIAGGRMHVVYAEGGHADEL
                     PVPPGTYTVITSRGFEYETDEQTVTVAADQTKTVTATLDRVVDTTGIQCGDFHVHTWR
                     SNDSGDTGLDKVRQAVADGVELPVRSDHEWVADFAWEIAQLGVEKFAAGFGSIELTSM
                     ELWGHMGVFPLTPDPTQPNAGAPKWQTFPTAAKLDEKFETLAPPVVFDAVRARPEAPV
                     VIINHPRGKTNYFGYVGYDPATGLASSVADWDTKFTLVEVFNNAGWQQNKNGTVVDWF
                     GLLKAGRKVFATGSSDSHHSSSSPVGYPRTCIALGTDDPRQLTANLVRDQLAAGHSTI
                     SGGIYVTAKIGTAGPGDTVNGAGSPMNVDVTIQAATWVDVDSIDVVVDGTIVDTIPVM
                     PGDADPQNPVIRWRKQIPIQAQATGGFVVIAAYGAGDLSPVHPGYKPFGMTNPIFVAP
                     "
     gene            complement(4549..6462)
                     /locus_tag="VMZ53_01365"
     CDS             complement(4549..6462)
                     /locus_tag="VMZ53_01365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27118.1"
                     /translation="MTRASALDAAARSEPGIPRAASSSEGIEDRRLTDSNQAWLGKIV
                     DGRYKVLEVIGRGGMGVVYRVEHLRMGKIAAMKVLHRDLASEPDVIARFEREAAAISK
                     LHHPHTVQVFDFGTAGTALYLIMEYVRGVDLARMIARDGPVPWHRAAALLAQVCSALQ
                     EAHEMQIVHRDLKPENVLITRTAGGRDYAKVLDFGLAKLEQRQAPSTMTDRQQIVGTP
                     YFMSPEQIRGDDVDHRSDIYSFGALMFEVLTGQHLFTGSTAVGVLTKHLTAEPDAPSM
                     RAPKMGIDPRVDHICRKALAKEPRDRWQTAAELAEAIEEVYAETVEHGTGPGGGRAVA
                     SKSLGRRGFHIAEEDTGESDLRLRRSDIDAFERGLKRQKYFSLSILVVLILGSAGAGA
                     WFVMREKPPLTEEAEPNNDGNHANKIAVGDVTGFIGKRMSPQDGDRDVFVVPWQSGER
                     HVVSVKVSGLPNLDINLTLNDGDGMHGQTADDGRVGEGEVMHRRSVDGPLVVTISETV
                     PKGTLPVENISDAYTLTVIDETTAGEREPNATDADANALELTQEMRGYLDSRNDVDEL
                     RFMGPDGTYNVIIRADGLPLAWSLGDDKLRTPGSAKVKLAHGALLRLERTDRSSTGPL
                     PARDTMWSVVVMP"
     gene            complement(6626..7609)
                     /locus_tag="VMZ53_01370"
     CDS             complement(6626..7609)
                     /locus_tag="VMZ53_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27119.1"
                     /translation="MLRPSLALSLIAALAACGHNDKSPAPKKSGTILTALFKAADAEK
                     SPWRCAALDTPVVPDEDLGGWKAHAHSLTREGNELTIGVIADAGGDAGKTLAALGRLR
                     GQLDDAKPDLVISLGGMGSDASQIAATLGTISDHAEWPLVALPGDLESAAAQSEAIET
                     LRKRGARVLDGRLVRFIEAGGATLATIPGASARDRLVAGDDGCGWTADDIAKLVGELT
                     TRKGLRVLVTAEAPRAMTGVSKRGAAGEATGELALVAAQPVDLAVHAPSGPVSAPSAA
                     QSGTRDGAKVALTPGTADATTRLPDAHVASAGVLAIRGDRWSWKPLVDSSK"
     gene            7645..7806
                     /locus_tag="VMZ53_01375"
     CDS             7645..7806
                     /locus_tag="VMZ53_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27120.1"
                     /translation="MKPKKILFWAGVVASVGAAILAVVSLDDPKLNPVSAIALAAAAF
                     ALFKGSELG"
     gene            complement(7811..8713)
                     /locus_tag="VMZ53_01380"
     CDS             complement(7811..8713)
                     /locus_tag="VMZ53_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HUS27121.1"
                     /translation="MTRRSLAALLLCTLVASAHAQTATQTIRVKPKDSLDLIAAEYYG
                     DRQSAVFIIAENKLKDRRLPPYARLRIPVTRDIVTDRGDTFQTLAEKYLGDKARAQVL
                     AEYNELDISETPAIGTPLTIPFQVTHAAQGTESLASIASTYLGDAKQAEFIRKYNGLD
                     KTSIEKGESVLVPVLVRMRPGKGISVDADAKARNDQHRKAMASIEDVLPNAHAAWLMG
                     DFKSVKDLLVPFADKTELLDMKVAVDLNVLLGKAHMAFNETQPAIDAFKRVLARKSWH
                     RLSSFYESPKVLDAWKQAGGQLSE"
     gene            complement(8710..10035)
                     /locus_tag="VMZ53_01385"
     CDS             complement(8710..10035)
                     /locus_tag="VMZ53_01385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27122.1"
                     /translation="MEEVASGGMAVVYRAVQDPLGRTVAIKALKTSAAAEENVVTRFE
                     REAKSLANLQHENIIHVYDFHRERGAMFIVMEYVQGIDLYDLLEKSGRLPYDVAAIIC
                     MQVARALDYVHYRGIVHRDIKPANIMIARTGGVKVMDFGIARDTNLGDLTEAGTGIGT
                     PAYMSPEQVLGDKLDARSDIFSLGVVLYQMVTGKKPFVEDERKSAMHKIRLEKHESAR
                     AVNPEVPRELNNIIDKCLEKQPRDRWRSAQHMVMALERFLSRHVEMNHHARLVLFLKA
                     QGVITELEAEEYLNPAALGAGAGALQQPNLQARNAVRAGVIAHGVVFGVLLLFISLIH
                     VAPLDPTPRTQDVLRKEAKRGYARVDAHPWAHVLVDGKEVGTTPMADALPLTEGTHVV
                     RYEHDWYEPYDQEVDVVAGTKEAPGIFRIDWQQLERDGKVKLKSGKTKP"
     gene            10508..>10849
                     /locus_tag="VMZ53_01390"
     rRNA            10508..>10849
                     /locus_tag="VMZ53_01390"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1843 a         3529 c         3480 g         1997 t
ORIGIN      
        1 cagccggatc gccaacccga gatgggctcg ctatccgacg ccgccgtgct gccgcgcctg
       61 atctacgggt tcgcgccgag ctggcagaag gtgctcgagc ccgtcgccgg tcgttacctc
      121 gacaaggcgg tgccgaatcc gctgcccgag gcgatcaagg ccgaggccga tcgtgtcgtc
      181 gccggcgcga aggacgaagc ggagaaggcg cagaagctct acgcattcgt cgcgcacgac
      241 attcgctcga tcgatctgcc gctcggtgct gcgggctacg agccgcacgc gcccgaagtc
      301 gtgctgcaga accgctacgc cgatgaccgc gacaaagtcg gcctgctgct cgcgatggca
      361 tcgtcgcaag gcattcgcgg ccgcgcggtg ctcgtgcgca cgggcaaggt gcccgtcatc
      421 tcgagcgtgc cgacgctcgc gcaattcgat cgcatgatcg cgaagctcga tgtcggcggc
      481 aaagacacgt ggctcgatcc atccgacgag aacggccaat acgcggtcgc gttcgccggc
      541 caggacaacc tcgtgctgcc gctcgacaag ggcggcgtcg agctcggctc gcggcccgcg
      601 ctcgatccat cgacgtcggt ctcgtcgacg aaagcgacgt acacgctctc ggcgaacggc
      661 gacctcagcg cgaagtacac gtacgagctc tcgggctggt acgccgatcg cgcgcaggga
      721 gagcttcgtc cgctcaaggg cgagctcgcc gacaagttct tccagcagga agcggcgggt
      781 gtcgcggcat cggcgctcga caaaggccac accgtcagcg acacgcaatc cgtcacgggc
      841 gcggtcacgg tgacgcaaga cgtctcggtc cccggctact cgtcggcgca ggcgaacatg
      901 cgcgtgttcg agctgccacc ggtcgcgttc tccgtcgctg atgacgagcc atctgcctcg
      961 ctgtcgacgc gcaagacgcc gctatgggtc ggcgtgcctc gtacggaacg cggcgagatc
     1021 accgtgcaga ttcctgccgg ttggaaggtc gcgtacgtgc cgccgaagct cgagggctcg
     1081 gccgaaggcg tgaagttctc gtcggagtgc agcgcggccg gccagaccgt cacgtgcaaa
     1141 ggcgagatgg tgctcgacaa gctcgtcgtg ccggccgaca agtacggcgc gttccgcgag
     1201 gcgctcacga aactgcaggc gtacgagcgg cgcgtggtac tccttcagaa ggggtgagaa
     1261 gactcgcact gatctcgatc gtgctcgtcg ggtgcggccg catctcgttc gagccgcaca
     1321 cggcgcacga tgacggcgcg atcggcgacg tggtgctcga ccagcccggc gatgcgaccg
     1381 tcgatcccga cctgctgttc tacgtctcgt tcgacggagg tctcgccgac gatgcgcgtg
     1441 gccatgccct gtcgtgcatg ggcacatgcg actcgatccc cggccgggta ggccccaacg
     1501 cggtccactt gaatggcgcg tcgtgcattg ttcttcccga cgcaggcgat cttcatccgg
     1561 ccacgtacac cgtctcggtc tgggcgcgcc tgccggtacc ggccgcaaat tccgacctcg
     1621 ttgcgcatcc cttgaatggt gcaacctcgt ttatcgacac gttcgagctg tgggtgcagc
     1681 ccgacagcgg cgtcgtgatg ctcgcaaaca gcgtccaggt actcaccacc ggcctcgacg
     1741 tgacgacgtg gcaccattac gtcgccgtct acgacggcag cgcggtcctc tcgtacgtcg
     1801 acggcgggct cgccggagga agctccggta ccacgacgac gatgtacggc gccgatccgt
     1861 acctcatcgg ttgcgaccgc gataacggca cggagatcgc gcacctcacc ggtgatgtcg
     1921 acgacgtgcg cttctacggc cgcgcgctca ccgcgaacga gatcgtcgcc ctcgcgaatc
     1981 tctaaggcgc gacgaagatc ggattcgtca tgccgaacgg cttgtagccg ggatgcacgg
     2041 gcgagaggtc gccggcgccg tacgccgcaa tcacgacgaa tccgcctgtc gcctgcgctt
     2101 ggataggaat ctgcttgcgc cagcgaatca ccggattctg cggatcggcg tcaccgggca
     2161 tcaccggaat cgtgtcgacg atcgtgccat cgacgacaac atcgatcgaa tcgacatcga
     2221 cccaggtcgc ggcctgtatg gtcacgtcga cgttcatcgg cgagcccgcg ccattgacgg
     2281 tgtcgcccgg acccgccgtg ccgatcttgg cggtgacgta aatcccgccg gagatcgtcg
     2341 agtgacccgc cgcgagctga tcgcgcacga ggttcgccgt cagctggcgc ggatcatcgg
     2401 tgccgagcgc gatgcacgtt cgcggataac cgaccggtga gctcgacgaa tgatgcgagt
     2461 cactcgatcc cgttgcgaac actttgcgtc ccgctttgag gagcccgaac caatcgacga
     2521 cggtgccatt cttgttctgc tgccagccgg cgttgttgaa cacctcgaca agcgtgaact
     2581 tcgtgtccca atcagcgacc gagctcgcga gccccgtcgc ggggtcgtag ccgacgtagc
     2641 cgaagtagtt cgtcttgccg cgaggatgat tgatgatcac gaccggcgcc tcgggccgcg
     2701 cgcgcacggc atcgaacacg acaggtggcg cgagcgtctc gaacttctcg tcgagctttg
     2761 ctgccgtcgg gaacgtctgc cacttcggcg cgcccgcgtt cggctgcgta ggatcgggcg
     2821 tcagcgggaa cacgcccatg tgtccccaga gctccatcga tgtcagctcg atcgagccga
     2881 agcccgccgc gaacttctcg acaccgagct gcgcgatctc ccacgcgaag tcggcgaccc
     2941 attcgtgatc gcttcggacc ggaagctcga cgccatcggc aacggcctgg cgcactttgt
     3001 cgaggccggt atctcccgag tcattcgagc gccaggtgtg gacatggaag tcgccgcact
     3061 gaatgccggt tgtatcgacg acgcgatcga gcgtcgcggt caccgtcttc gtttgatcgg
     3121 cggcgaccgt caccgtctgc tcgtccgtct cgtactcgaa gccgcgcgac gtgatcacgg
     3181 tgtacgtgcc cggcggcacc ggtagctcat cggcgtgtcc gccctcggcg tagacgacgt
     3241 gcatgcggcc acccgcgatg tcgggctcac cgtaattcgc gggcggcggc gcagcagtcg
     3301 acaacacctg aatgcgcgcg ggaactgcgg caccgttctc ggtcgcgacg acgttgacgt
     3361 atccgacagg cggcagcgtg atcgtcatcg aatcgctcgt cgccggaaca tcgacaagcc
     3421 cggtctcgcg cgtgtacgcg cgtagcgtga tgtcggcgcc agccggcacg tgcaacgtga
     3481 agccaccacg agcgccctgc gtcgtcgcgc gcgagaggta gtggcccgcg gcgtcgatcg
     3541 cgtgaacgtg gacgccgagc aggttctggc cgccgcgttg cacggtcccc gtgatctcgc
     3601 gctcgcggtt ctcgcccgtc agcttcgcag cggcggtctg caccggatcg agtcctggtc
     3661 cgccgacgat gagcttcaca gcgaggcggt cgaattcgct gccgcacggc ggaatcgtgt
     3721 agcccggtcc gaacgcgccg agaaatccgc tcgccgcgat cgagctgccg agcgtgcctt
     3781 caccgggcat gtacgcccag ctcgtggcgt tgtcctcgac gaacgcgacg tagggtgtcg
     3841 agctcagctg cgagtcgaag ccgacgttcg gttgaaacac cgtcggtgac gtgcgccccg
     3901 agtacatgag gccgtgcatc gtcgacggca ctttggtttc catcgagcgc ggcgagtgat
     3961 agcggacgcg gatgtcgacg tggtccgcat cgggtgcgag ctcgtaatcg atcgcggctt
     4021 cgatgtcggt gtactcgtcg ttgaacaaga tcgccgttag cggttcgaag aacggcaacg
     4081 ggtgcagctt gccgcgcgca cgaatgatcg cggtgccgcc gtggctgcca tcgttgatca
     4141 cgctcacgga ctcggtcacg accgtcgagc gtcccgtgag gatgaagaac tcgccgaagt
     4201 tgctcggctc cgcgatgtgc ccgttctcga cgcgcgccag accgacgggg cgcccgcccc
     4261 acggatcgta gagatcgcta tcgccgacgt cttcgatcac gagtgcgacg cggttgttcg
     4321 cgatcacgaa gtcgttgtcg gcccacgtca gcaactgggg aacgccgacg ccgggaggcg
     4381 cgagctgctc gccgtgaatg cgaccggcgc gcgcttctcc tggtcctgca ccgagtggct
     4441 ctgcgtgacc ggttggatct ccgtcggcaa acggcagcgc gcacgtcgcc gcgtcatcac
     4501 cgcagccgtg catcagaagt gcaatgaatg aaacgaatgc gacgcgattc aaggcatcac
     4561 caccacggac cacatcgtat cgcgcgccgg tagcggaccg gtgctcgatc tgtcagtgcg
     4621 ttcgaggcgg agtagtgcac catgtgcgag cttcactttc gcagacccag gcgtgcggag
     4681 cttgtcgtcg ccgaggctcc acgcgagtgg caggccgtct gcgcgaatga tcacgttgta
     4741 ggtgccgtcg ggtcccatga agcgcagctc gtcgacgtcg ttgcggctgt cgagataacc
     4801 gcgcatctcc tgcgtgagct cgagcgcgtt cgcatcggca tctgtcgcat tcggctcgcg
     4861 ctcgccggca gtcgtctcgt cgatcaccgt cagcgtgtac gcgtcgctga tgttctcgac
     4921 cggaagtgtg cctttcggca cggtctcgct gatcgtgacg acgaggggac cgtcgacgga
     4981 gcggcgatgc atcacctcac cttcgccgac gcgtccgtca tcggcggtct ggccatgcat
     5041 gccgtcgcca tcgttgagcg tcaggttgat gtcgaggttc ggtaggccgc tgaccttcac
     5101 gctcacgacg tgtcgctcgc ccgactgcca cggcacgacg aacacatcgc gatcgccgtc
     5161 ttgcgggctc atgcgcttgc cgatgaatcc agtcacgtcg ccgaccgcga tcttgttcgc
     5221 gtgattgccg tcgttgttcg gctccgcttc ttccgtcagc ggcggcttct cgcgcatcac
     5281 gaaccacgcg cctgcacctg cactgccgag gatgagcacg acgaggatcg agagcgagaa
     5341 atacttctgg cgcttgagcc cgcgctcgaa cgcgtcgatg tcgctgcggc gcaggcgtag
     5401 gtcgctctcg ccggtgtcct cttcggcgat gtgaaagcca cgccggccga gtgacttcga
     5461 cgcgacggct cgtccgccgc ccggaccggt gccgtgctcg acggtctccg cgtacacctc
     5521 ttcgatcgct tccgcgagct cggccgcggt ctgccagcga tcgcgcggct ccttcgccag
     5581 cgccttgcga cagatgtgat cgacgcgcgg atcgatgccc atcttcggtg cccgcatcga
     5641 cggcgcatcg ggctccgccg tcaggtgctt cgtcagaacg ccgaccgcag tcgagcccgt
     5701 gaagagatgc tgaccagtca ggacttcgaa catcagcgcg ccgaacgaat aaatgtcgct
     5761 gcgatgatcg acgtcgtcgc cgcgaatttg ctcgggcgac atgaagtagg gcgtgccgac
     5821 gatctgctgg cgatccgtca tcgtcgacgg cgcttgccgc tgttcgagct tcgcgagccc
     5881 gaagtcgagc accttcgcgt aatcgcggcc gccggccgtg cgcgtgatca gtacgttctc
     5941 gggcttgaga tcgcgatgca cgatctgcat ctcgtgcgct tcttggagcg cgctgcacac
     6001 ctgcgcgagg agagcggcgg cgcgatgcca cggcacgggc ccgtcacgcg cgatcatccg
     6061 ggcgaggtcg acgcctcgca cgtactccat gatcaggtag agcgcggtgc ccgccgttcc
     6121 gaagtcgaag acctggacag tgtgcgggtg gtgcagcttc gagatcgccg ccgcttcgcg
     6181 ctcgaatcgc gcgatcacat cgggctcgct cgcgagatcg cgatgcaaga ccttcatcgc
     6241 cgcaatcttg cccatgcgca gatgctcgac gcgatacacg acgcccatgc cgccgcgtcc
     6301 gatcacctcg agaaccttgt aacggccatc gacgatcttg ccgagccacg cctgattcga
     6361 atcggtcagc cgccggtctt cgattccttc gctgctcgaa gcggcacgcg gaataccggg
     6421 ctcactgcgt gccgctgcat ccagcgcact cgcacgtgtc atgtccgcgc cgcactcgcg
     6481 gcagaagtga tggagctccc cgaaggaggt ccgacaccgc ggacagacga atttttgtgc
     6541 cggtgtcgac gacacgagat gtcgatctta ccgccccaaa gcgcgcgcgc ccattacttc
     6601 gcggcgacac gccgctgtcc gattgctact tgctgctatc gaccagcggc ttccagctcc
     6661 accgatcccc gcgaatcgcg agcacaccgg ccgacgcaac atgcgcatcg ggcaaccgcg
     6721 tcgttgcatc tgcggttccg ggagtcagcg cgaccttcgc gccatcacgc gtcccgcttt
     6781 gcgcagcgct cggagcactg accggcccgc tcggcgcatg cacagcgaga tccacgggtt
     6841 gcgccgcgac cagcgcgagc tcaccggtcg cttcgcccgc cgcacctcgc ttacttaccc
     6901 cggtcattgc gcgaggcgcc tccgccgtga cgagcactcg cagccccttc cgcgtggtca
     6961 gctcgccgac cagcttcgca atatcgtccg cggtccaacc gcagccatcg tcgccggcaa
     7021 cgaggcggtc gcgtgcgcta gcgcccggta tcgtcgcgag ggtcgcgcca ccggcctcga
     7081 taaagcgcac cagccggcca tcgaggacgc gtgccccgcg cttgcgcagc gtctcgatcg
     7141 cctcgctctg cgcggccgcc gactccagat caccgggcaa cgcgacgagt ggccactccg
     7201 catgatcgct aatcgtcccg agtgttgcgg caatctgcga cgcatcgctg cccatcccgc
     7261 cgagtgagat cacgagatcg ggtttcgcgt cgtcgagctg gccgcgcaag cgtccgagtg
     7321 cggcgagcgt ctttccggca tcgccacccg catccgcgat gacgccgatc gtgagctcgt
     7381 tcccctcgcg cgtgagtgag tgcgcgtgtg ccttccaacc gccgaggtcc tcatcgggca
     7441 ccacaggcgt atcgagtgca gcgcagcgcc acggtgactt ctccgcgtcc gccgctttga
     7501 acagcgcggt gagaatcgtc cccgatttct tcggcgccgg cgacttgtcg ttgtgcccgc
     7561 acgccgcgag cgcagcgatc agcgacagtg caagactcgg gcggagcacg acgtgactgc
     7621 ttataacgcg agtaccatca gctcatgaaa ccgaagaaga tcctgttctg ggctggcgtg
     7681 gtggcgagcg tgggtgcagc gattcttgcc gtcgtgtcgc tcgatgaccc gaagctgaac
     7741 ccggtgagtg cgatcgcgct cgcagcagct gcgttcgcgc tgttcaaagg ctccgagctc
     7801 ggctgacgcg ttactcggac agctggccgc cggcctgctt ccacgcgtcg agcactttcg
     7861 gcgactcgta gaacgagctg aggcgatgcc agctcttgcg cgcgagcacg cgcttgaacg
     7921 catcgatcgc gggctgcgtc tcgttgaacg ccatgtgcgc tttgccgagg agcacgttga
     7981 gatcgacggc gaccttcatg tccagcaact cggtcttgtc ggcgaacgga acgagcaggt
     8041 ctttgaccga cttgaagtcg cccatgagcc acgccgcatg cgcgttcggc agcacgtcct
     8101 cgatggatgc catcgccttg cgatgttgat cgttgcgcgc ctttgcgtcg gcatcgacgc
     8161 tgatcccttt gccgggccgc atccgaacga gcacggggac gagcacggac tcgcccttct
     8221 cgatgctcgt tttgtcgagg ccgttgtact tgcggatgaa ttcggcttgc ttcgcgtcgc
     8281 cgagatacgt cgatgcgatc gacgcgagcg actcggtccc ctgcgctgcg tgcgtgacct
     8341 gaaatggaat cgtcagcggc gtgccgatcg cgggcgtctc cgagatgtcg agctcgttgt
     8401 attcggcgag cacttgcgcg cgcgccttgt caccgaggta cttctccgcg agcgtctgga
     8461 aggtgtcccc cctgtcagtg acgatgtcac gcgtcaccgg aatgcgaagg cgcgcgtacg
     8521 gcggcaggcg gcgatccttc agcttgttct cggcgatgat gaagacggcc gactggcgat
     8581 cgccgtagta ctcggcggcg atgagatcga gcgagtcttt cggtttcacg cggatcgtct
     8641 gcgtcgcggt ttgcgcgtgc gccgaagcga cgagcgtgca gaggagcagg gctgcgagcg
     8701 agcgtcgcgt cacggcttcg tcttcccgga cttgagcttc accttgccgt ctcgctcgag
     8761 ctgctgccaa tcgatgcgga agatgccagg cgcttccttc gtgccggcga cgacgtcgac
     8821 ctcttgatcg taaggctcgt accaatcgtg ctcgtagcga acgacgtgcg tgccttccgt
     8881 gagtggcagc gcgtcggcca tcggggtcgt gcccacttct ttgccatcga cgagcacgtg
     8941 cgcccacgga tgagcatcaa cgcgtgcgta cccgcgcttc gcctccttgc ggagcacgtc
     9001 ctgcgtgcgc ggcgtcggat cgagcggcgc gacgtggatg agcgagatga agagcagcag
     9061 cacgccgaac acgacgccgt gtgcgatgac gcccgcgcgg actgcattgc gtgcttgcag
     9121 attcggttgc tgcagcgcgc cggcgccggc accgagcgcc gctggattca aatactcctc
     9181 ggcctcgagc tcggtgatca cgccttgtgc tttgaggaac agcacgaggc gcgcgtgatg
     9241 attcatctcg acgtgtcgcg agaggaatcg ctcgagcgcc atgaccatgt gctgcgcact
     9301 gcgccaccga tcgcgcggct gtttctcgag gcacttgtcg atgatgttgt tgagctcgcg
     9361 cggaacttcg gggttcaccg ctcgcgcact ctcgtgcttc tcgagccgga tcttgtgcat
     9421 cgccgatttg cgctcgtcct cgacgaaggg cttcttgccc gtgaccatct gatagagcac
     9481 gacaccgagc gaaaagatgt cgctgcgcgc gtcgagcttg tcgccgagaa cttgctcggg
     9541 agacatgtac gcaggcgtgc cgatgccggt tccagcctcg gtgagatcgc cgaggttcgt
     9601 gtcgcgcgcg atgccgaagt ccatcacctt cacgccaccg gtgcgcgcga tcatgatgtt
     9661 cgcgggcttg atgtcgcgat gcacgatgcc gcggtagtgc acgtagtcga gagctcgcgc
     9721 gacctgcatg cagataatcg cggcgacgtc gtacggcaga cgaccgctct tctcgaggag
     9781 gtcgtacagg tcgatgccct gcacgtactc catgacgatg aacatcgcgc cgcgttcgcg
     9841 atggaagtcg taaacgtgga tgatgttttc gtgctgcaga ttcgcgaggc ttttcgcttc
     9901 gcgctcgaag cgcgtgacga cattttcctc tgcagctgcg ctcgttttga gcgctttgat
     9961 cgccacggtg cggccgagcg gatcctgcac ggcgcgatag acgacagcca tgcctccgct
    10021 ggctacctcc tccacgatcc gacaatttcc gatgcgctcg agcatggaat ccggcctgag
    10081 gttacagtgg tttgcgccga aaacggtgca cgcgaacgac ccatttgcca ggacaccgtc
    10141 ccgtggtaac taaggacccg taggcggagc gggcgacgga gccatcatcg cgagatcttc
    10201 atcaggaaac tgatgaaaaa acggtgacgg ttcaatggga tgcgggtcag gtcgcaggtg
    10261 ggacggattt tttttcgatc ccagttgacg actaaaaact gatcccgtat aagcagcatc
    10321 cctcgacggc cccaagcctt cgagaccgac gagaagaacg cttccgtcgg gatcggtctc
    10381 tgaaaactgg atagcaagcc aatttaggaa gattgagttt ctgttcgaaa gaaaccattc
    10441 cacgttggac gactacctcc ccccgggggc ggtctatcag ccaacgtaga gcgggattca
    10501 aaacttttct atggagagtt tgatcctggc tcagagcgaa cgttagcggc gggcttaaca
    10561 catgcaagtc gagcgagaaa agggggcaac ccctgagtaa agcggcggac gggtgagtaa
    10621 cacgtaggta atcttccctt gagcggggga caacgaggcg aaagcctcgc taataccgca
    10681 tacgtttctt tggactctgg tctgaagaag gaaagctagg ccgcaaggtc cggcactcga
    10741 ggaggagcct gcgtcgcatc agctagttgg tggggtaacg gcctaccaag gcgaagacgc
    10801 gtagctggtc tgagaggatg atcagccaca ctgggactga gacacggcc
//
LOCUS       DATLBZ010000029         9448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4493336, whole genome shotgun sequence.
ACCESSION   DATLBZ010000029 DATLBZ010000000
VERSION     DATLBZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4493336"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VMZ53_01395"
     CDS             <1..901
                     /locus_tag="VMZ53_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HUS27123.1"
                     /translation="SKMDLANQVMADIKTFKETNNCSRLVAVWCGSTEVYRMPGDVHS
                     SLAKFEAGLERSDDDIAPSQIYAYACLKMGVPYANGAPNLSIDTPALLELARKQGLPV
                     TGKDFKTGQTLMKTILAPGFKARMLGLEGWYSTNILGNRDGEVLDDPESFKSKEVSKL
                     GVIDSILQPHLYPELYGNVSHVVRINYYPPRGDNKEGWDNIDIVGWLGYPMQIKVNFL
                     CRDSILAAPIVLDLALFMDFAARAGMSGIQEWLSFYWKSPMTPEGLYPEHDLFIQLMK
                     LKNTLRYTKGDELITHLGAEYYD"
     gene            911..1654
                     /locus_tag="VMZ53_01400"
     CDS             911..1654
                     /locus_tag="VMZ53_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27124.1"
                     /translation="MRLLAFLLVLATGCRYGGTFTCETSDQCGTNGTCEPTQFCSFPD
                     SMCALGQRYDETAGPYAGACVGEEPRPDGGIDAPDGAPFDLTSCPSGFGAVPNLPGSR
                     YAIHTPNGTFWAHHAYCNALLPGATHLLIPDTAQELDILASRLSTTVTYAYVGAVQDA
                     ATATLPNAAWFKLDGQPLDAALWGTGDPDDDDDIETDHYSQTALLSRASRRLDDAGGA
                     TARPALCECDGKSVAASVEAIIAADPNKN"
     gene            complement(1651..2175)
                     /locus_tag="VMZ53_01405"
     CDS             complement(1651..2175)
                     /locus_tag="VMZ53_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HUS27125.1"
                     /translation="MPQLADPNFSRAVVLMIEHGDQGSFGLVINHPSPITATELLESL
                     EMSWHGDDSAVVWAGGPVSPSTGWVLHEPVGVAHAGTGGTIEITSTISLSTSPDRLRA
                     IAKTPPRNVRLLLGYSGWGPGQLAQEMARGAWLHTAADPSIVFETPPDEIWDRAMRSL
                     GINPRDLFTGKGVN"
     gene            complement(2261..2446)
                     /locus_tag="VMZ53_01410"
     CDS             complement(2261..2446)
                     /locus_tag="VMZ53_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27126.1"
                     /translation="MQLFLRAVVTGFGLSLGSALYKKAAKRFGFDDDDKPKKDEETKR
                     TNQQDGGTDPGLAISAS"
     gene            complement(2464..3663)
                     /locus_tag="VMZ53_01415"
     CDS             complement(2464..3663)
                     /locus_tag="VMZ53_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27127.1"
                     /translation="MVALRKHLLITTAMLMTLALAGAIVVRAHQRPQRGRPASVVGVG
                     AYFHGGTPARDEELEQFLVEDLTQLLIETDADRMGSREGAPRARRVSELRNAKVIAAR
                     GPGLANAWRELIDALDRWADLPVRGPRVQRAIDELGKRAQAVSNEFARLGVGYYLQAD
                     AMLGKTAHAIVFVYRVEQVTFVHVGGQRRRVLDLRRLDQLNLDLAMLGRQNDESPDPV
                     VLLDRIDHFIDGKLAGVLASDTSAGLEIADELRAAGATDREAIREIVTATVRRHEARH
                     GLDLDRLDAGRPLRYPKALAALAGPRSMATLRAELELAAYLSQIGNEPIAPKLALWNL
                     TTVGSNPQRRKSAEAYAARVVLDGLSRKLDVRVGSLTAQSDENLRAAARSLWVDLYGE
                     PMLPITD"
     gene            3712..4260
                     /locus_tag="VMZ53_01420"
     CDS             3712..4260
                     /locus_tag="VMZ53_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27128.1"
                     /translation="MGRWFSLVVAALWLSATTPADACPAGSPCLKYRAQTVTQQPDHF
                     AREGGGTIPKFDRKKLARFLATSKWKPVFGDGNGPAAKTPTRYHVTYVKDSSKLRFVD
                     PSGKIPTAGKDERVILIRQIERDNEGNYFIDVDGIAYQLWYCVSGKRTSPCLTNSASA
                     FSSMFPLDKQDPAQPLLKPMPF"
     gene            complement(4257..5999)
                     /locus_tag="VMZ53_01425"
     CDS             complement(4257..5999)
                     /locus_tag="VMZ53_01425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27129.1"
                     /translation="MPEPTVSLEDPGAGTARTASDEHTEIDGTEIGTQAASPKALADA
                     AAVGSHAATELGEAKPSGWATASARSGANPPTAAEAFDKSDLARSQMFHIFGIFAPLG
                     ALVLSILIGGDPTARHLFWMGAGLISLGNIGLVYLTSKPERYNATGIGILWIFSTIGV
                     QPAIYYFGPFSAVVMVDMLGIVFIALGRVRWTARATAGICIGGHVLISVPIIAGWIDD
                     VGVLPSVVTRREQLIVAEILVVGFLYAGYALGRWARSTAATALAELQSAMRIIGDREQ
                     ALAEVVDEAARQKRVNEGRWTNQKLGRWRLGVVLGRGAMGEVYEAMGDDGRPAALKLL
                     NARSTSSSAIVERFHREMDVAARLESPHIVKVFEISAPDAPVPYIAMERLHGNDLATR
                     LRNENQNRMASDELVILIDQVARGLEVARLAGVVHRDLKPHNLFHHDGSVWKILDFGV
                     SKVLDSEGTLTGEGIVGTPQYMAPEQASGGHVTHLADVYALGAIAYRCLTGRSPFKGK
                     DLAELVYQVVHTPPLRPSMLGRVSSSIEDVLAVAMAKDPRKRFPSALSFAQAFIAARR
                     GRAIVVDIPDNPWT"
     gene            6024..6740
                     /locus_tag="VMZ53_01430"
     CDS             6024..6740
                     /locus_tag="VMZ53_01430"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HUS27130.1"
                     /translation="MGAQWKHAGRLASSTAKGRIFSKLAKELAIAAKSGADPTLNARL
                     RAVIEAAKKVSMPKDTLERAIKKGAGLLDERIEYETVTYEGFGPHRVPVIVECLTDNN
                     KRTSTNIRHHFRNGQLSPVAWDYAHVGLVDASPAEGASAPSAEQIEEAAIEAGAQDFE
                     AGAADVEGGVRFYTEPTDVDAVAKALSAHGWTVATMRIGWRPKNPVAVPDAAARTEVE
                     QFLHELDEDDDVQYIYPALA"
     gene            6967..7851
                     /locus_tag="VMZ53_01435"
     CDS             6967..7851
                     /locus_tag="VMZ53_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HUS27131.1"
                     /translation="MRDDGTGDDDGTGSGSGSGSGSGDMGTPRQCDKMDIVFVVDNSG
                     SMQEEQSNLASNFPMFASLLQGYQVNGGPLDFRIAVTTTGRTIDYQIDLGGGFGTFPQ
                     HEDGQNGAFQNNCNSTKRWLEPTDPNMAQTLACRANVGTTGPSIEMPLLMSKWSLKER
                     IADNTNAGFLRDDALLAIVVLTDEDDASTSQNNFTMDASGSSPTDWNPADEVMFLDTL
                     KGNRTRWAAGVIAGEGDCSSSFGNAANGARLKDFVMQANGQGSTQAVFSSICEGNLTN
                     GLKDALDTFQAACGQIIL"
     gene            7873..8556
                     /locus_tag="VMZ53_01440"
     CDS             7873..8556
                     /locus_tag="VMZ53_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS27132.1"
                     /translation="MDIVVTGGTGALGAGVVELFLSRGATVHLPMMEAALPAHLPWRE
                     HERVKATPGISLDDEAQVTSFYASLPALWASIHLVGGFSMAKLAETTLADFERQWRMN
                     TVTCFLTCREAVKAMRRTGSAGGRIVNVAARPAITPTAGMISYVASKAGVAALTQSLA
                     PELVSEGILINAVLPSTIDTPANRKSMPNADHASWPKPAQIAETIAFLASAENALTTG
                     TLIPVFGRA"
     gene            8612..9340
                     /locus_tag="VMZ53_01445"
     CDS             8612..9340
                     /locus_tag="VMZ53_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUS27133.1"
                     /translation="MSDKPDLDRYPELREKWDRRNFIRTAAAGTALMAVGGALARLAA
                     DDLTKQARAEKRPDGRPRLPPGQRVIENLRPMGGDEGDGDAKTFKLKVHGLVKKPFEV
                     GYEGLLKLPQVEKSADVHCVTGWTMLGGLYKGVQISKLAEIAEMKSEARYVILESWHG
                     FTANVPLKEATADNALITYRLNGKPFALQHGAPVRALVPDLYFWKSAKWITGIKFVKD
                     DQPGYWEVRGYHNHADPWKEERYG"
BASE COUNT         1734 a         3153 c         3005 g         1556 t
ORIGIN      
        1 gtcgaagatg gacctcgcca atcaggtgat ggccgacatc aagacgttca aggagacgaa
       61 caactgctct cgtctcgtcg cggtgtggtg cggctcgacc gaggtctatc gcatgccggg
      121 cgacgtccac agctcgctcg cgaagttcga agccggcctc gagcggtccg acgatgacat
      181 cgcaccgtca cagatctacg cgtacgcgtg cctgaagatg ggcgtgccct acgccaacgg
      241 cgcgccgaat ctctcgatcg atacgcccgc gctcctcgag ctcgcgcgca agcaaggcct
      301 gcccgtcacc ggcaaggact tcaagaccgg ccagacgctg atgaagacga tcctcgcgcc
      361 cggcttcaag gcgcgcatgc tcggcctcga gggctggtac tcgacgaaca tcctcggcaa
      421 ccgcgatggc gaagtgctcg acgatcccga gtcgttcaag agcaaggaag tgtcgaagct
      481 cggcgtgatc gattcgatcc tccagccgca cctctacccg gagctgtacg gcaacgtcag
      541 ccacgtcgtt cgcatcaact actacccgcc gcgcggcgac aacaaggaag gctgggacaa
      601 catcgacatc gtcggttggc tcggctaccc gatgcagatc aaggtgaact tcctctgccg
      661 cgactcgatc ctcgccgcgc cgatcgtcct cgatctcgcg ctgttcatgg acttcgcggc
      721 gcgcgcgggc atgtcgggta tccaggagtg gctgtcgttc tactggaagt cgccgatgac
      781 gcccgaaggc ctgtatcccg agcacgactt gttcatccag ctcatgaagc tgaagaacac
      841 gctgcgctac accaagggtg acgagctcat cacccacctc ggcgccgagt actacgatta
      901 gaatcggcga gtgaggctcc tcgccttcct cctcgttctc gcgaccggct gccgctacgg
      961 cggaacgttc acgtgcgaga cgagcgatca atgcggcacg aacggtacgt gcgagccgac
     1021 gcagttctgc agcttcccgg attcgatgtg cgcgctcggg cagcgctatg acgagacggc
     1081 tgggccgtat gcgggcgcgt gcgtcggaga agagccgcgg cccgacggtg gaatcgatgc
     1141 gccggatgga gcgcccttcg atctgacgtc atgcccttca gggtttggcg cggtgccgaa
     1201 cctcccgggc tcgcgctatg cgattcacac accgaatggc acgttctggg cgcatcacgc
     1261 gtactgcaat gctctgctgc ccggcgcgac acacctgttg atccccgaca ccgcgcaaga
     1321 gctcgacatt ctcgcttcgc gcctctcgac gacggtgacg tacgcgtacg tcggtgcggt
     1381 ccaggatgcg gccacagcga cgttgcccaa cgcagcgtgg ttcaagctcg acgggcaacc
     1441 actcgatgcg gcgctctggg gaaccggcga cccggatgat gacgacgata tcgagacgga
     1501 ccattactcg cagacggcgc tgctgtcgcg cgcttcgcga cggctcgacg atgcaggcgg
     1561 tgcgactgcg cgcccggcgc tctgcgagtg cgacggcaaa tcggtagcag cgagcgtcga
     1621 ggcgatcatc gccgccgatc cgaacaagaa ctagttgacg cccttgcccg taaacaggtc
     1681 gcgcgggttg ataccgagcg agcgcatcgc gcgatcccag atctcgtcgg gcggcgtctc
     1741 gaacacgatc gacggatcgg ccgcggtgtg cagccacgcg ccgcgcgcca tctcttgcgc
     1801 gagctgcccc ggcccccagc ccgagtagcc aagcagcaat cgaacattgc gcggcggcgt
     1861 cttggcgata gcgcgcaagc gatcgggcga ggtcgacagc gagatcgtcg acgtgatctc
     1921 gatcgtgccg ccggtacctg cgtgcgcgac gccgaccggc tcgtgcagca cccagccggt
     1981 gctcgggctg accggaccgc cagcccatac gacggcagaa tcgtcgccgt gccagctcat
     2041 ctcgaggctc tcgagaagct cggtggccgt gatcgggctc gggtggttga tgacgaggcc
     2101 gaacgaccct tggtcgccgt gctcgatcat cagaaccacg gcgcgcgaaa aattggggtc
     2161 ggccagctga ggcatcgcca gcagtagccc tggtgccagg ttcgccgact ccactggtct
     2221 ccagtgttcc gtaaaaccgc gcgagatgct tcaaaaaaag ctaggacgcc gaaatcgcta
     2281 gtccgggatc cgtgccgcca tcctgttgat tcgtgcgctt tgtctcttcg tcctttttcg
     2341 gcttgtcgtc atcgtcaaag ccgaagcgct tggctgcctt cttgtagagc gccgagccga
     2401 gcgagagacc aaaaccggtg acgactgcgc gcaggaaaag ctgcatgcct cgaatgctag
     2461 cagctagtcc gtgatcggca gcatcggctc gccatagagg tccacccaca ggctgcgcgc
     2521 ggcggcacgc agattctcgt cactctgcgc agtgagggaa ccgacgcgga cgtcgagctt
     2581 gcggctgagg ccatcgagca cgacgcgcgc tgcgtaggct tcggcactct tgcggcgttg
     2641 tgggttactg ccgaccgtcg tgaggttcca gagcgcgagc ttcggcgcga tgggttcatt
     2701 gccgatctgc gagagatacg cggcgagctc gagctctgcg cgtagcgtcg ccatgctgcg
     2761 tggaccggcg agcgctgcga gcgccttcgg ataacgcagc gggcgccctg cgtccaagcg
     2821 gtcgaggtcg aggccgtggc gcgcttcgtg ccggcgcacc gtcgccgtga cgatctcgcg
     2881 gatcgcttcg cgatcggttg cgcccgcagc gcgcagctcg tcggcgatct cgagaccggc
     2941 gctcgtatcg ctcgcgagca cacccgcgag ctttccgtcg atgaagtgat cgatccgatc
     3001 gagaaggacg acgggatcgg gcgactcgtc gttctgccgg ccgagcatcg cgagatcgag
     3061 gttgagctga tcgagccggc gcaagtcgag cacacgacga cgctgccctc cgacatgcac
     3121 gaacgtgact tgctcgacgc gatacacgaa cacgatcgcg tgcgccgtct tgccgagcat
     3181 cgcgtctgcc tgcaggtagt agccgacgcc gagccgcgcg aattcgttcg acaccgcctg
     3241 cgcacgtttg ccgagctcgt cgatcgcgcg ctgcacccgc ggtccacgca ccggcagatc
     3301 ggcccagcga tcgagcgcat cgatcagctc gcgccacgcg ttcgccagcc ccggaccacg
     3361 cgcggcgatc accttcgcat tgcgcagctc gctgactcgt ctcgcacgcg gtgcgccttc
     3421 gcgcgagccc atgcgatcgg cgtcggtctc gatgagcagc tgcgtgaggt cctcgacgag
     3481 aaactgctcg agctcttcgt cccgcgcggg cgtgccgccg tggaagtagg cgccaacgcc
     3541 gacgaccgac gcgggcctgc cgcgctgtgg gcgttggtgt gcgcgaacga cgatcgctcc
     3601 cgcaagcgcc agggtcatca gcatcgccgt cgtgatcagc agatgcttgc ggagggcgac
     3661 caccaaaaga tgacaactgc accgatggcc ggtttcacct aagatcgagg aatggggcga
     3721 tggttcagtc tggtcgtcgc ggcgctgtgg ctttctgcca cgacacccgc cgatgcatgc
     3781 ccggccggct cgccatgtct caagtaccgg gcgcagaccg tcacgcagca gcccgaccac
     3841 ttcgcgcgcg aaggcggcgg cacgatcccg aagttcgacc gcaagaagct cgcgcgcttc
     3901 ctcgcgacct cgaagtggaa gccggtcttc ggcgatggca acggtcccgc cgcgaagacg
     3961 ccgacgcgct atcacgtcac gtacgtcaaa gactcgtcga agctccggtt cgtcgatccg
     4021 agcggcaaga tcccgacagc gggcaaagac gagcgcgtga tcctgattcg ccagatcgag
     4081 cgcgacaacg agggcaacta cttcatcgac gtcgatggaa tcgcgtacca gctctggtac
     4141 tgcgtgtccg gaaagcggac gagtccgtgc ctgacgaaca gcgcgagcgc gttcagcagc
     4201 atgttcccgc tcgacaagca ggatcccgcg cagccgctgc taaagccgat gccgttctag
     4261 gtccacgggt tgtcggggat gtcgacgacg atcgcccgac cgcggcgcgc cgcgataaat
     4321 gcctgcgcga acgacagcgc cgacgggaat cgcttgcgcg ggtccttcgc catcgcgacc
     4381 gcgagcacat cttcgatcga cgacgacacg cgtccgagca tgctcgggcg aagcggcggc
     4441 gtgtgaacca cttgatagac aagctccgcg aggtccttgc ccttgaacgg tgagcggccg
     4501 gtcaggcagc gatacgcgat cgcgccgagt gcgtagacat cggcgaggtg cgtgacgtga
     4561 ccgccggacg cttgctccgg cgccatgtac tgcggcgtgc cgacgatgcc ttcgcccgtc
     4621 agcgtgcctt cactgtcgag gactttcgac acgccgaagt cgaggatctt ccagaccgag
     4681 ccatcgtgat gaaacaggtt gtgcggcttg agatcgcgat gcacgacgcc cgcgagccgt
     4741 gcgacttcga gcccgcgtgc gacttgatcg atcaagatga ccagctcgtc gctcgccatg
     4801 cggttctggt tctcgttgcg caggcgcgtc gcgaggtcgt tgccatgcag acgctccatc
     4861 gcgatgtaag gcaccggtgc gtcgggcgcg gaaatctcga acaccttgac gatgtgcggt
     4921 gactcgagcc gagcggcgac gtccatctcg cgatgaaatc tctcgacgat cgcactgctg
     4981 ctcgtcgagc gcgcgttgag gagcttgagc gcggcaggcc tgccgtcgtc gcccattgct
     5041 tcgtagacct cgcccatcgc gccgcgtccg agcacgacgc cgagtcgcca gcggccgagc
     5101 ttctgattcg tccagcggcc ttcgttgacg cgcttttggc gcgcggcctc gtcgacgact
     5161 tcagcgagcg cctgctcgcg atcgccgatg atacgcatcg cggactgcag ctcggcgagc
     5221 gcggtggcgg ccgtactgcg cgcccaccgg ccgagtgcat agcccgcgta cagaaagccg
     5281 acgacgagga tctcggcgac gatcaactgc tcgcgtcgcg tgacaacgct tggcagcacg
     5341 cccacgtcgt cgatccatcc cgcgatgatc ggcacggaga tgaggacgtg gccgccgatg
     5401 cagatgcccg ccgtcgctcg cgccgtccag cgcacgcggc cgagcgcgat gaagacgatg
     5461 ccgagcatgt cgaccatcac gacggcggag aacgggccga agtagtagat ggccggctgc
     5521 acgccgatcg tggagaagat ccagaggatc ccgattcccg tcgcgttgta tcgctcgggc
     5581 ttgctcgtca gataaacgag gccgatgttg ccgagcgaga tcaggccagc acccatccag
     5641 aagaggtgcc gcgccgtcgg atcgccgccg atgagaatcg acagcaccag cgcaccgagc
     5701 ggcgcgaaga tgccgaagat gtgaaacatc tggctgcgcg cgaggtcgct cttgtcgaac
     5761 gcttctgctg cggtcggcgg attcgcgccg ctgcgtgccg acgcggtggc ccaccccgag
     5821 ggcttcgctt cgccgagctc ggtagcagcg tgcgagccca cggcggcggc gtccgcgagc
     5881 gcctttgggg atgccgcctg cgtcccgatc tcggtgccgt cgatctcggt gtgctcgtcc
     5941 gatgcggtcc tcgcagtccc ggccccggga tcttcgaggc tcaccgtcgg ttcgggcacg
     6001 gatcgatgct agaagggcaa gccatgggcg cgcaatggaa acacgcggga cgcctcgcca
     6061 gctccacggc gaagggccga attttcagca agctcgcgaa ggagctcgcc atcgctgcaa
     6121 aaagcggagc cgacccgacg ctcaacgctc gccttcgcgc cgtgatcgag gcggccaaga
     6181 aggtctcgat gccgaaggac acgctcgagc gcgcgatcaa gaaaggcgcc ggcctcctcg
     6241 acgagcgcat cgagtacgag accgtcacct atgaaggctt cggcccgcat cgcgttcccg
     6301 tgatcgtcga gtgcctcacc gacaacaaca agcgaacgtc gacgaacatc cgccaccact
     6361 ttcgcaacgg ccaactgagt cccgtcgcgt gggactacgc gcacgtcggt ctcgtcgatg
     6421 cgtcgcctgc agaaggcgcg agcgcaccgt ccgccgagca gatcgaagaa gccgcgatcg
     6481 aagccggcgc gcaagacttc gaagcaggtg ccgccgatgt cgaaggtggc gtgcgctttt
     6541 atactgagcc gaccgatgtc gacgccgtcg cgaaggcgct ctcggcacac ggctggaccg
     6601 tcgcgaccat gcgaattggc tggcggccga agaaccccgt agccgtcccg gatgcagccg
     6661 cgcgaaccga ggtcgagcag ttcctgcacg agctcgatga ggacgacgac gtccagtaca
     6721 tctatcccgc gctcgcctga acacgccgga ccgcggaccg aacgcgccac cgaacctccc
     6781 gaaacgtgag gtcagatcca gcgttcttgc acaacttgcg caaagcgcag gccgccaccg
     6841 aatctgattc cgatcgcgtt ttgccgtgtg tatcgtcgcc gcacttttga gggggctcaa
     6901 atgatgtcga agactgctgc gtttgttttt gcaagcatct ctatggttgc cgcgtgcggt
     6961 cccgcgatgc gagacgacgg cacgggcgac gacgacggaa ccggctccgg ctcgggctct
     7021 ggctcgggct ccggtgacat gggcacaccg aggcagtgcg acaagatgga catcgtgttc
     7081 gtcgtcgaca actcgggctc gatgcaagag gagcaatcga acctcgcgtc gaacttcccg
     7141 atgttcgcca gcctcctcca gggctaccaa gtgaacggcg gcccgctcga cttccgcatc
     7201 gccgtcacga cgaccggccg cacgatcgat taccagatcg atctcggcgg cggtttcggc
     7261 acgttcccgc agcacgagga cggccagaac ggcgcgttcc agaacaactg caactcgacg
     7321 aagcgttggc tcgagccgac cgacccgaac atggcgcaga cgctcgcctg ccgcgcgaac
     7381 gtcggcacga cgggcccatc gatcgagatg ccgctcctca tgtcgaagtg gtcgctcaaa
     7441 gagcgcatcg ccgacaacac gaacgccggc ttcctccgcg acgacgcgct cctcgcaatc
     7501 gtcgttctca cggacgaaga cgacgcctcg acgtcgcaga acaacttcac gatggacgcg
     7561 tcgggcagct cgccgaccga ctggaacccg gccgacgagg tgatgttcct cgacacgctc
     7621 aagggcaacc gcacgcgttg ggccgctggc gtcatcgcgg gcgaaggcga ctgctcgtcg
     7681 agcttcggca acgccgcgaa cggcgcgcgc ctcaaagact tcgtcatgca ggcgaacggt
     7741 cagggctcga cgcaggccgt cttctcgtcg atctgcgaag gcaacctgac gaacggcctc
     7801 aaggacgcgc tcgacacgtt ccaagccgcc tgcggtcaga tcattctctg atgtgataca
     7861 gcactccgcc tgatggacat tgttgtcaca ggcggaacgg gcgccctcgg cgctggcgtt
     7921 gtcgagctgt tcctctcgcg tggtgccacg gtgcacctgc cgatgatgga ggccgcgctg
     7981 ccagcgcatc tcccgtggcg cgagcacgag cgcgtcaaag cgacgcccgg catctcgctc
     8041 gacgacgaag cgcaggtgac cagcttctac gcatcactgc ctgcgctgtg ggcgtcgatt
     8101 catctcgtcg gcggcttctc gatggccaag ctcgccgaga ccacactcgc cgacttcgag
     8161 aggcagtggc gcatgaacac cgtcacgtgt ttcctcacgt gtcgcgaagc ggtgaaggcg
     8221 atgcgtcgca cgggctctgc aggtggtcgc atcgtcaacg tcgcggcgcg ccctgcgatc
     8281 acgccgaccg ccgggatgat ctcgtacgtc gcgtcgaaag ccggcgtggc cgcgctcacc
     8341 caatcgctcg cgccggagct cgtctccgaa ggcatcttga tcaatgccgt gctcccgtcg
     8401 acgatcgaca cgccggccaa ccgcaaatcg atgcccaacg ccgaccacgc gagctggcca
     8461 aagccggccc agattgccga gaccattgcg tttttggcct ccgcagagaa cgcgctgacg
     8521 accgggacac tcattcccgt gttcggccga gcgtagtcct cattcgtaac cttcatgtgg
     8581 ccgcgccgta tgccacgatg aaggagtcac cgtgagcgac aagcccgatc tcgatcggta
     8641 tcccgagctt cgcgagaagt gggaccgccg caacttcatc aggacggccg ccgcaggcac
     8701 cgcactcatg gcagtcggcg gcgcactcgc acgcctcgcc gcggacgacc tgacgaaaca
     8761 agcgcgcgcc gaaaagcgcc ccgacggccg ccctcgcctg ccgcccggcc aacgcgtgat
     8821 cgaaaacctc cgcccgatgg gcggcgacga aggcgacggc gacgccaaga ccttcaagct
     8881 caaggtccac ggcctcgtga agaaaccatt cgaggtcggc tacgaaggcc tgctgaaatt
     8941 gccgcaggtc gagaagtccg ccgacgttca ctgcgtgacc ggctggacga tgctcggcgg
     9001 tctctacaaa ggcgtgcaga tttcgaagct cgccgagatc gccgagatga agtccgaagc
     9061 tcgctacgtg atcctcgagt cgtggcacgg cttcaccgcc aacgtcccgc tcaaggaagc
     9121 gaccgccgat aacgcgctga tcacctatcg cttgaacggc aagccattcg cgcttcagca
     9181 cggagccccc gttcgcgcgc tcgtgccgga tctgtatttc tggaagagcg cgaagtggat
     9241 cacgggcatc aagttcgtca aagacgatca gcccggctac tgggaagtcc gcggctatca
     9301 caaccacgcc gacccgtgga aggaagagcg gtatggctga tccggcgtcc ggcgccggtc
     9361 tcctgccact catcaagaaa cgttggcgcg catggctccg cgccgtacat cgcgacgtcg
     9421 gctacctcgc gatcggcttc acggtcat
//
LOCUS       DATLBZ010000030         8673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8962715, whole genome shotgun sequence.
ACCESSION   DATLBZ010000030 DATLBZ010000000
VERSION     DATLBZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8962715"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..875
                     /locus_tag="VMZ53_01450"
     CDS             126..875
                     /locus_tag="VMZ53_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS27134.1"
                     /translation="MAGSQLCAGRVAFVTGGSRGLGRAICLSLAREGAFVAFNYAKAD
                     ADADETLAQITAAGGRGSAHKVSVLDKAGLRELVRVLEQTHGKIDILVNNAGFGQVVP
                     LALMEESDWDDMLDTHVKGAFLTTQAVLRIMVRERYGRVINVGSLAGIKMMQAPVHYA
                     TAKAALKGFTESLAKEIGRYGITVNCVAPGILDEGVSDHLPPARKEEYVRHCALRRVG
                     RLEEAADMIAFIASERASYTNGATLVVDGAV"
     gene            917..2140
                     /locus_tag="VMZ53_01455"
     CDS             917..2140
                     /locus_tag="VMZ53_01455"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS27135.1"
                     /translation="MPEVRPQSPIARLVGARSLNTFGRSIISATVLWELYERTGSKYV
                     IAAVGAIQVLPVVLLFVPVGALVDRSDRRSLTTLCAMAAGVIGIGLATASYFAAPVAV
                     YLGLLLAFGCSTAVMAPASVSLIPLIIERPDLERTNRISSSLQEIAAISGPALSGLAL
                     KVVDAQWVYAAVAVTGIAAGTLYRSLPKPRDVSVTNASATRKDWRTGLRFIFRSPLLL
                     PALTLDMFAVLFAGVTALLPAVAHDILHVDAFGYGILRAAQSVGAVLSAVITGRMPPW
                     KRPGRVLLIVVALFGLATIGFGLSTSFPLSAALLVACGALDNISVVIRLTLEQMVVPD
                     AIRGRVAAVHFVFIGMSNELGAAESGVAAGLLGTVPAIVGGGALAMLVVAGVALKWRE
                     LAAMPPLSELKPADE"
     gene            complement(2141..2725)
                     /locus_tag="VMZ53_01460"
     CDS             complement(2141..2725)
                     /locus_tag="VMZ53_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27136.1"
                     /translation="MKTLTCGLALMLAVAAGCKGNSKSSGDGLAKLSGFKDEMCRCKD
                     AACAQDVSSKMTTWTQEQAKGDHAGKLSDSDTAKAQKISDELGACMAKAMTATAGGGS
                     AAVGSAGSAAPVDPAAFKDLPQDCLDYRATVDKLASCGDKLPADVRDTLKQRFDESVQ
                     GWAKLPPEAKKSLGPACKAGVQAVEANAKAQCGW"
     gene            complement(2867..3313)
                     /locus_tag="VMZ53_01465"
     CDS             complement(2867..3313)
                     /locus_tag="VMZ53_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27137.1"
                     /translation="MKKLTLAMMVAMAFVSFGCKKKAGSDSANAMAKMQEFADEMCKC
                     KDAKCAQDVSDKMTKWGQEQSKNQKEPPKMNEADTAKAQEIGKKMGECMTKAMGAGGA
                     MGGGTPEAGSATPTEGSAAPAAGSGDMGSAAAAGSAGSAAAGSAAP"
     gene            complement(3397..5307)
                     /locus_tag="VMZ53_01470"
     CDS             complement(3397..5307)
                     /locus_tag="VMZ53_01470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HUS27138.1"
                     /translation="MKKETKRFTEECLAGLHRAQELRPQIIASTSRTIDGTLAPYNEL
                     LMAASATNALAGLMSEVHPDEGIRDAARECEQEVSRFYSDLALDRGMYDALSAVDVSK
                     ADADTKRFVEHTLRDYRRAGVDKDEATRDRLKAIDEELTRLGQIFSKNISEDVRSVET
                     NDVSRLAGLPVDFIEAHPPDDKGIIRITTDYPDYNPFMTYAMDDELRRDLYIKFRSRG
                     DQQNEDVLHHILELRREKANLLGFKNWADYITADKMIQSGDRASEFIEKVWKLAAPRS
                     EEDYKELLRQLKSIDPAAKEVADWQKVWLENLVKRERYEVDASEVRQYFPYDRVLKGL
                     LEITSEIFDIAYVAIADKAWHASVLTYDVMRGSDKLGRIYLDMHPREGKYKHAAQFPL
                     KDGVRGKQLPEGVLVCNFPDPKSGGDALMEHDDVVTMFHEFGHLMHHVLGGHQRWITQ
                     SGVATEWDFVEAPSQMFEEWAWRHDTLSRFARHVKTGEVIPESLVENMRRADKFGLGT
                     ATVQQIFYAAISLGFHRADPDKLDQLGEVQKLQKQYTPFRYVPGTRFHASFGHLVGYS
                     AMYYTYQWSLVIAKDLLTPFEQKGLMAKDVTLAYRDKVLAPGGSRDAAELVRNFLGRD
                     YDFAAYERYLVS"
     gene            5335..5922
                     /gene="miaE"
                     /locus_tag="VMZ53_01475"
     CDS             5335..5922
                     /gene="miaE"
                     /locus_tag="VMZ53_01475"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HUS27139.1"
                     /translation="MIEALGDALPLVPTGPGWLEVALSDLDAVHADHLHCERKAAQSA
                     LSLIRSYPDHTDLVEAMARLAHDETRHVIQVAQLMQRREQPSSFDHGDEYASALREHV
                     RKREPDRLLDRLLVFAVIEGRSAERLGLLADALPDERDRALYGSLATAEVRHRDIFLS
                     LARDIAPDSWRARAAELAQIEAEILAVKPVMPRIH"
     gene            6049..>8673
                     /locus_tag="VMZ53_01480"
     CDS             6049..>8673
                     /locus_tag="VMZ53_01480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27140.1"
                     /translation="MIPNDQTIATPPSYPIEITANAPPNIPKAFAKRDPRSQTGRKKG
                     DSAQALDFSKNKQIGRFELIREIARGGMGQVFLGRDTKLGRKVAVKFLLRDDPHFVQR
                     FLVEARATARCAHENIVTIYEVGEHEGLPYMVLEFLEGKTLSDVLEGKPSLRQFVELM
                     VPVARALERAHEHGIVHRDLKPSNIFVTDRGSVKVLDFGVARLIDRAESDIVRATAAM
                     EAVSLDEMNSSSTFTGSNSLVGTLPYMSPEQWGAAEVDHLSDIWAFGIMFWRALTGVH
                     PAGTMHPDRLRERLCDLNTPLPSIVVRDPTLPRDLAAIIDKCLAKRKSDRYQSATQLL
                     ADLQAFLQPKADRTTGEVCPYRGLAAFGEDDAKYFFGRSNEIRTAIAQLEQWPMLAVI
                     GPSGVGKSSFVHAGLVPAIRAAGGNWQVRVLRPGRVPLHRLASVLEESLATGQPAADF
                     MQGLLEAPGMFGQQLRAAATRHNANILVVVDQLEELFTLCDSDDVRKLFLAALLAAAD
                     DATSPVRVVLSMRADFLDRLAHHKHFLSELSRGLFFLTAPDQDNLRETLERPAELAGY
                     TFENPGIVEDMMQAATSRGALPLLSFAATRLWDARDRQRRMLTVAAYNQMGGVGGAFA
                     RHADQVASSVPPQTQLLLRAIMTRLVTPEGTRAVVDHRELLGLSPDTQEVERILDMLV
                     RARLIHMQTDPGDQGATVEIVHEMLITEWPTLKRWLEDSQAMRGFMHELRQAAKQWAS
                     RGKPADLVWRGATAQEALGHVKRHVLDLSTGEREFLAEVKAQTARSRRRKVAVFTAIA
                     VTLALVLAGGSFALVRIKLAEKDAQDKAAQAIKAKKEAEGALGQAKQATEEAVKAKGD
                     LQGKLDIIEAEKKQRLAAE"
BASE COUNT         1423 a         2900 c         2750 g         1600 t
ORIGIN      
        1 cagatcgacg gtcgcatcat gccggcgtgt ggcttcaaca tgttccatcg cggcgccgcg
       61 aagggaccga acacgccgcg cgcgcaattc ggccgcgctc cgttctcgct gccggtgatc
      121 acctagtggc gggctcgcaa ctctgtgcag gtcgcgtcgc attcgtgacg ggcggctcgc
      181 gtggcctcgg tcgtgcgatc tgtctgtcgc tcgcgcgcga aggcgcgttc gtggcgttca
      241 actacgccaa ggcggacgcc gacgccgacg agacgctggc gcagatcaca gcggcgggcg
      301 gtcgtggctc cgcgcacaag gtctctgtcc tcgacaaggc gggcctacgc gagctcgttc
      361 gcgtcctcga gcagacgcac ggcaagatcg acatcctcgt caacaacgcc ggcttcggtc
      421 aggtcgtgcc gctcgccctc atggaagagt cggactggga cgacatgctg gacacgcatg
      481 tcaaaggcgc gttcctcacg acgcaggcag tccttcgcat catggtgcgc gagcgctatg
      541 gacgcgtcat caacgtgggc tctctcgcag gcatcaagat gatgcaggcg ccggtgcact
      601 acgcgactgc aaaggctgcg ctcaaaggct tcacggagtc gctcgcgaaa gagatcgggc
      661 gctacggcat caccgtcaat tgcgtcgcgc cgggcatcct cgacgagggc gtgagcgatc
      721 acttgccgcc cgcgcgcaaa gaagaatatg tgcggcactg cgcgctgcgc cgcgtcggcc
      781 gtctcgagga agccgcagac atgatcgcgt tcatcgcgtc agagcgcgcg agctacacga
      841 acggcgcgac gctcgtcgtc gacggcgcag tctagccatt cacgccagca gtcccgcgcg
      901 ctagcatccg agcgcagtgc ctgaagtccg accgcagagc ccgattgctc gactggtcgg
      961 cgctcgctcg ctcaacacgt tcggccgctc gatcatcagc gcgaccgtcc tctgggagct
     1021 ctacgagcgc actggcagca agtacgtcat cgcggcggtc ggcgcgatcc aagtgctgcc
     1081 cgtcgtgctg ctgttcgttc ctgtcggcgc gctcgtcgat cgcagcgatc gccggagcct
     1141 caccacgctc tgtgcgatgg ccgccggcgt gatcggcatc ggcctcgcaa cggcgtcgta
     1201 cttcgcagcg cccgtcgcgg tgtacctcgg cctcttgctc gcattcggtt gctcgaccgc
     1261 ggtgatggct ccggcgagcg tgtcgctgat tcctctcatc atcgagcggc cggatctcga
     1321 gcgtacgaat cgcatcagct cgagcctgca agagatcgcc gcgatctcgg ggcctgcact
     1381 ctccggcctc gcgctcaagg tcgtcgacgc gcagtgggtc tacgcagcgg tcgccgtcac
     1441 gggtatcgcc gcgggcacgc tctatcgctc gctaccgaaa ccgcgcgacg tcagcgtcac
     1501 gaacgcatcg gcgacgcgca aagactggcg caccggcctg cgcttcatct tccggtcgcc
     1561 gctgcttctg cccgcgctca cgctcgacat gtttgcggtc ttgttcgccg gcgtcaccgc
     1621 gctattgccc gctgtcgcgc acgacatcct gcacgtcgat gcgttcggct acggcatttt
     1681 gcgcgcagcg caatcggtcg gtgccgtgct cagtgccgtc atcaccggcc gcatgccgcc
     1741 gtggaagcga ccggggaggg tgctgctgat cgtcgtcgcg ctgtttggtc tcgcgacgat
     1801 cggctttggg ctctcgacgt cgtttccact ctctgctgcg ctgctcgttg cgtgcggcgc
     1861 gctcgacaac atcagcgtcg tgattcgact gacgctcgag cagatggtcg tccccgatgc
     1921 gattcgcgga cgcgtcgccg ccgttcactt cgtcttcatc gggatgtcga acgagctcgg
     1981 tgctgcagag tcgggcgtcg ctgcaggtct gctcggcacc gtgcccgcga tcgtcggcgg
     2041 tggtgcgctc gcgatgctcg tcgttgccgg cgtcgcgctg aagtggcgag agctcgcggc
     2101 gatgccgccg ctgtccgagc tcaagcccgc cgacgagtag ctaccagccg cactgcgctt
     2161 tcgcgttcgc ctcgacggct tgcacgcccg ctttgcacgc cggaccgagg ctcttcttcg
     2221 cttccggcgg aagcttcgcc caaccttgca ccgactcgtc gaagcgttgc ttcagcgtgt
     2281 cgcgcacatc ggcgggcagc ttgtcgccgc acgatgcgag cttgtcgacg gtcgcgcggt
     2341 agtcgagaca gtcctgcggg agatccttga acgccgccgg atcgacgggc gctgcactgc
     2401 ccgcgcttcc caccgctgcg ctgccaccac cggccgtcgc cgtcatcgcc ttggccatgc
     2461 acgcgccgag ctcgtcgctg atcttctgcg ccttcgcggt gtcggagtcg ctgagcttgc
     2521 cggcgtgatc gcccttggct tgctcttgcg tccacgtagt catcttcgac gagacgtcct
     2581 gcgcgcacgc ggcgtctttg cacctgcaca tctcgtcttt gaagccgctc agcttcgcga
     2641 gcccatcgcc ggagctcttg ctgttgccct tgcaacccgc tgccaccgcc aacatcaacg
     2701 cgagcccgca cgtgagcgtc ttcatcgtcg aagatctagc gcagaacgcg atccgcaggg
     2761 gaatgtaacg acttgtaatc ggactcgtaa cgaatgctcg caaaaaaaaa cgggccccaa
     2821 aggacccgct tatcaaagcg aactagatct gatcgctagc tgaagcttag ggagctgccg
     2881 agccagccgc cgccgaacca gccgagccag ccgccgccgc cgagcccatg tcgcccgagc
     2941 cagccgccgg agccgccgaa ccctcggtcg gggtcgccga gccagcctcg ggggtgccgc
     3001 cgcccatcgc gccgccggcg cccatggcct tggtcatgca ctcgcccatc ttcttgccga
     3061 tctcttgggc cttcgccgtg tccgcctcgt tcatcttcgg cggctctttc tggttcttgg
     3121 actgctcctg gccccacttc gtcatcttgt cagagacgtc ctgggcgcac ttggcgtcct
     3181 tgcacttgca catctcgtcg gcgaattcct gcatcttcgc catcgcattt gccgagtcgc
     3241 tgcctgcctt cttcttgcag ccgaacgaaa caaaggccat cgcaaccatc atcgcaagcg
     3301 tgagcttctt catctgtgag tcctctcctt agggaacgac agaatgaaaa atctgcggtc
     3361 gccgcgcaac gaatcaactg tattgctttg tcgcgactag ctgacgagat atcgctcgta
     3421 cgctgcaaag tcgtaatcgc ggcccaagaa gttgcgcacc agctcggccg catctcggct
     3481 gcctccaggc gccaggacct tgtcgcggta agcgagcgtc acgtccttgg ccatcaagcc
     3541 tttttgctcg aatggagtca gcaggtcttt cgcgatcacg agtgaccact gatacgtgta
     3601 atacatcgcc gaataaccca ctaaatgccc gaacgacgcg tggaaccgcg tacccgggac
     3661 atacctgaac ggcgtgtact gcttctgtaa cttctgaact tcgccgagct gatcgagctt
     3721 gtcgggatcg gcgcggtgga agccgagcga gatcgccgca tagaagatct gctgcacggt
     3781 cgcggtgccg aggccgaact tgtccgcgcg ccgcatgttc tcgacgagcg actcggggat
     3841 gacctcgccg gtcttcacgt gccgagcgaa gcgcgagagc gtgtcgtggc gccaggccca
     3901 ctcctcgaac atctgcgacg gtgcttcgac gaagtcccat tcggtcgcga caccgctctg
     3961 cgtgatccag cgctgatggc cgccgagcac gtgatgcatg aggtggccga actcgtggaa
     4021 catcgtgacg acgtcgtcgt gctccatgag cgcgtcgccg cctgacttcg gatcggggaa
     4081 gttgcagacg agcacgccct cggggagctg cttgcctcgc acgccgtctt tgagcgggaa
     4141 ctgcgcggcg tgcttgtact tgccttcgcg cggatgcatg tcgaggtaga tgcggccgag
     4201 cttgtcgctg ccgcgcatca cgtcgtacgt gagcacgctg gcgtgccacg ccttgtcggc
     4261 gatcgcgacg tacgcgatgt cgaagatctc gctcgtgatc tcgagcaggc ccttcagcac
     4321 gcggtcgtat gggaagtact gccgcacctc actcgcatcg acctcgtagc gctcgcgctt
     4381 gacgagattc tcgagccaca ccttctgcca atcagcgact tccttcgccg ctggatcgat
     4441 cgacttcagc tggcgcagca gctccttgta gtcttcctcg gagcgcggcg cggcgagctt
     4501 ccagaccttc tcgatgaact ccgacgcgcg atcgccggac tggatcatct tgtcggcggt
     4561 gatgtagtcc gcccagttct tgaagccgag gaggtttgcc ttctcgcgac gcagctcgag
     4621 gatgtgatgg aggacgtcct cgttctgttg atcgccgcgg ctgcggaact tgatgtagag
     4681 gtcgcgacgg agctcgtcat ccatcgcgta cgtcatgaac gggttgtagt cgggataatc
     4741 cgtcgtgatg cggatgatgc ccttgtcgtc gggcggatgc gcttcgatga agtcgacggg
     4801 caggcccgcg aggcgcgata cgtcgttcgt ctcgacggag cggacgtcct cggagatgtt
     4861 cttcgagaag atctggccga ggcgcgtgag ctcttcatcg atcgccttga gccgatcgcg
     4921 cgtcgcttcg tctttgtcga cgcccgcacg ccggtagtcg cgcagcgtgt gctcgacgaa
     4981 ccgcttcgta tcggcatcgg ccttcgagac gtcgacggcg gacagcgcgt cgtacatccc
     5041 gcggtcgagc gcgaggtccg aatagaagcg cgagacttcc tgctcgcact cgcgcgccgc
     5101 atcgcggatg ccttcgtcgg gatgtacctc cgacatgagg ccggccaacg cgtttgtcgc
     5161 gctcgccgcc atcagcagct cgttgtacgg agcgagcgtg ccgtcgatcg ttcgcgatgt
     5221 gctcgcgatg atctgtggac gtaattcctg cgcgcggtgt aagccggcga ggcactcttc
     5281 ggtgaaccgc ttcgtctcct tcttcaccga cgcagatgat acacatcgat cgacgtgatc
     5341 gaggcgcttg gtgacgcact tccgctcgta cccacggggc ccgggtggct cgaggtcgcg
     5401 ctgtcggacc tcgatgcggt gcacgcagac cacctgcatt gcgaacgcaa agcggcacag
     5461 tcggcgctgt cattgattcg gagctacccg gatcacacgg atctcgtcga ggcgatggcg
     5521 cgacttgcgc acgacgagac tcgccacgtc attcaggtcg cgcagctgat gcagcgccgc
     5581 gagcaaccgt cgtccttcga ccacggcgac gaatacgcgt ctgcattgcg tgagcacgtc
     5641 cgcaagcgcg aaccggaccg tctactcgat cggctcctcg tgtttgcggt catcgaaggc
     5701 cgctccgccg agcgactggg cctgcttgcc gacgcacttc ccgacgagcg cgaccgtgcg
     5761 ctgtacggaa gccttgcgac agcggaggtc cggcaccgcg atatcttctt atctcttgcg
     5821 cgcgatatcg cgccggattc gtggcgtgca cgtgcggcgg agctcgcgca gatcgaagcc
     5881 gaaatcctcg cggtaaagcc ggtcatgccg cgaattcatt gagatggtac cattcccgac
     5941 tgcgtggccg gaagggatga cgacgatccg cgagcgcagc gaccgcagcg cgctccgacg
     6001 gcaaggacga tcgctgccgt acctgcgcca cgtggtgatg cgacgtccgt gatcccgaat
     6061 gatcagacga tcgcgacgcc gccgtcgtat ccgatcgaga tcaccgcgaa cgcgccgccg
     6121 aatattccga aggcattcgc aaagcgcgat cctcgctcgc agacgggccg caagaagggc
     6181 gattcggcgc aggcgctcga cttctcgaag aacaagcaga tcggccgctt cgagctgatc
     6241 cgcgagatcg cacgcggcgg catgggccag gtcttcctcg gtcgcgacac gaagctcggc
     6301 cgcaaggtcg cggtgaagtt cttgctccgc gacgatccgc acttcgtgca gcgcttcctc
     6361 gtcgaggcgc gtgcgacggc gcgctgcgct cacgagaaca tcgtcacgat ctacgaggtc
     6421 ggcgagcacg aaggcttgcc gtacatggtc ctcgaattcc tcgagggcaa gacgctgtcc
     6481 gacgtgctcg agggcaagcc gtcgctgcgg cagttcgtcg agctgatggt gccggtggcg
     6541 cgagcactcg aacgcgcaca cgagcacggc atcgttcacc gcgacctcaa gccatcgaac
     6601 atcttcgtca ccgatcgcgg ctccgtgaag gtcctcgact tcggcgtcgc gcgcctcatc
     6661 gatcgcgccg agtcggacat cgttcgcgcg accgccgcga tggaagcggt ctcgctcgac
     6721 gagatgaatt cctcgtcgac gttcaccggg tcgaactcgc tggtcggcac gctgccgtac
     6781 atgtcaccgg agcagtgggg cgccgcggag gtcgatcacc tctcggacat ctgggcgttc
     6841 ggcatcatgt tctggcgcgc gctgaccggc gtccacccgg cgggcacgat gcatcccgat
     6901 cgtctgcgcg agcgcttgtg cgatctcaac acgccgctgc ccagcatcgt ggttcgcgac
     6961 ccgacgctgc cgcgagacct tgccgcgatc atcgacaagt gcctcgcgaa gcggaagagc
     7021 gaccgctatc agagcgcgac gcagctgctc gccgacctcc aggcgttcct gcagccgaag
     7081 gcggatcgca cgaccggcga agtctgcccg tatcgcggtc tcgccgcgtt cggcgaagac
     7141 gacgcgaagt acttcttcgg ccgctccaac gagattcgca cggcgatcgc gcagctcgaa
     7201 caatggccga tgctcgccgt gatcggtccg tccggcgtcg gcaagtcgtc gttcgtgcac
     7261 gccggtctcg tgcccgcgat ccgcgcggca ggcggcaact ggcaggtgcg cgtgctgcgg
     7321 cccggtcgcg tgccgctcca tcgcctcgcg agtgtgctcg aagagtcgct ggcgacgggg
     7381 caacctgccg ccgacttcat gcagggcctg ctcgaagcgc cgggcatgtt cggtcaacaa
     7441 ctgcgcgccg cggccacgcg tcacaacgcg aatatcctcg tcgtcgtcga tcagctcgaa
     7501 gagctgttca cgctctgcga cagcgacgac gttcgcaagc tgttcctcgc cgcgctgctc
     7561 gccgccgccg acgatgcgac ctcgcccgtt cgcgtcgtgc tgtcgatgcg cgctgacttc
     7621 ctcgatcgac tcgcgcatca caagcacttc ctcagcgagc tgtcgcgtgg gctgttcttc
     7681 ctgacggcgc ccgatcaaga caacctgcgc gagacgctcg agcgcccggc cgagctcgcc
     7741 ggctacacgt tcgagaatcc gggaatcgtc gaggacatga tgcaggccgc gacgtcacgc
     7801 ggtgcgctgc cgctgctgtc gttcgccgcg acgcgcctgt gggacgcacg cgatcgccaa
     7861 cggcggatgc tcacggtcgc tgcgtacaac caaatgggcg gcgtcggtgg tgcgttcgcg
     7921 cgacacgccg atcaggtcgc atcgtcggtg ccgccgcaga cccagctcct gctgcgcgcg
     7981 atcatgacgc ggctcgtgac gcccgaaggc acgcgcgctg tcgtcgatca ccgcgagctt
     8041 ctcgggctgt cgccagatac gcaggaagtc gagcgcatcc tcgacatgct cgtgcgcgcg
     8101 cgcctgatcc atatgcagac ggatccgggc gatcagggcg caaccgtcga gatcgtgcac
     8161 gagatgctga tcaccgagtg gccgacgctc aagcggtggc tcgaagacag ccaagcgatg
     8221 cgaggcttca tgcacgagct gcggcaagcg gccaagcagt gggcgtcgcg cggcaagccc
     8281 gcggacctcg tgtggcgtgg cgcaaccgcg caagaggccc tcggccacgt gaagcgccac
     8341 gtcttggatc tctcgaccgg ggagcgcgag ttcctggcgg aggtgaaggc gcaaacggct
     8401 cgcagccggc gtcgcaaggt cgctgtgttc accgccatcg cagtgacact cgccctcgtt
     8461 ctcgcaggcg gcagctttgc gctcgttcgt atcaagctcg ccgagaagga cgcgcaggat
     8521 aaggcggcac aagcgatcaa ggcgaagaag gaagccgaag gcgcgttggg gcaggccaag
     8581 caagcgaccg aagaagcggt caaggcgaag ggcgacctgc aaggcaagct cgacatcatc
     8641 gaagccgaga agaagcagcg cctcgccgcc gag
//
LOCUS       DATLBZ010000031         2512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2259901, whole genome shotgun sequence.
ACCESSION   DATLBZ010000031 DATLBZ010000000
VERSION     DATLBZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2259901"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VMZ53_01485"
     CDS             <1..712
                     /locus_tag="VMZ53_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27141.1"
                     /translation="HRASDDRLAQLAATPVGADTSVEAWEQVPEDWWFLGDDMRDQGK
                     QDLVARYFGLHRAMFRGGNAYKLLGRMDVKPTYHYEFEDPQCMDEIDKLALPELLGRD
                     IRALHHSFADAVVEIERFGKLKTLDLTVTFLGTEPPMPAKTLYIARWDHGLTEGYTAR
                     MKRKGRAREREIVLDPKLKQSPWQIYLTIVGQPPKLLGTSTDDKPLKYVPTLRGQYWT
                     LACKPDYCFILSVTVHSI"
     gene            complement(681..1997)
                     /locus_tag="VMZ53_01490"
     CDS             complement(681..1997)
                     /locus_tag="VMZ53_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27142.1"
                     /translation="MALRPRLILLAGWLGFMLYAFPGYMSYDSVYQLDEARWGIFSDG
                     HPPAMAAMWRHVDAMIAGPLGMLIIQSVVFLAGMYLIFRERMKPNAAAFAAVFLLWFP
                     PVAAFMAVIWKDSQMTAFILLGTGLILQKQRWVRVSGLVMLAVGTAMRHNALTITLPI
                     VFLCFVWNPDYRWWKRYAIALVAWFGLTMSARVVSNALTDQHRYLWTDSIAMCDMAAT
                     LRYTSPTIPDAELRETFEGLRLWPDSDFHEFARRGDEEAEYVPTLWNTAYGLFAVPRD
                     EAERNAMVRAWKKIVLTHREEYLTYRWKMFTRLLGFGGDDSSPVYNWFTDIQDPYGSA
                     TMADHDAHAGKLQDVLRRGMHAVGRTPLFRVYVYLALLFILLPFARDRLTLAVLGSAA
                     SSEVILFLIAPTTDWRYSSWMILAVVIAAMLIAAQRIRSSAPSHSG"
     gene            2045..>2512
                     /locus_tag="VMZ53_01495"
     CDS             2045..>2512
                     /locus_tag="VMZ53_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUS27143.1"
                     /translation="MRVLIIVPAFNEEEALGGLLAELRALPAPATDVTLETVVVDDGS
                     SDRTADVARSGGARVLRLCGNLGIGGAVQSGLRLAHRERFDMAIQLDGDGQHPPDQLA
                     PLIASARTASPPDLVIGTRYSDKNNFRSTLLRRLGSWWLRVILRVVTRTRLSDP"
BASE COUNT          467 a          828 c          815 g          402 t
ORIGIN      
        1 gcacagagca tccgatgacc gcctcgcgca gctcgcagcg acgcccgtcg gggccgacac
       61 gtcggtcgag gcatgggagc aggtccccga agactggtgg ttcctcggcg acgacatgcg
      121 cgatcagggc aagcaggatc tagtcgcgcg ctacttcggc cttcatcgcg cgatgtttcg
      181 cggcggcaat gcctacaagc tgctcggtcg catggacgtc aagccgacgt accactacga
      241 gttcgaagat ccgcaatgca tggacgagat cgacaagctc gcgcttcccg agctactcgg
      301 ccgcgacatc agggcgctgc accactcgtt tgccgacgcc gtcgtcgaga tcgagcggtt
      361 cggcaagcta aagacgctcg acctgacggt gacgttcctc ggcaccgagc caccgatgcc
      421 ggcgaagacg ctctacatcg cgcgctggga ccacggcctc accgagggct acacggcacg
      481 gatgaagcgc aaggggcgtg cgcgcgagcg cgagatcgtg ctcgatccaa agctgaagca
      541 atccccatgg cagatctatc tgacgatcgt cgggcagccg ccgaagttgc tcgggacctc
      601 gacggacgac aagccgctga agtacgtacc gacgctgcgc gggcagtact ggacgctcgc
      661 gtgcaagccc gactactgct tcatcctgag tgtgacggtg cactcgatct gatgcgctgc
      721 gcggcgatca gcatcgctgc gatcacgacc gcgagaatca tccacgacga gtaacgccag
      781 tcggtcgtcg gtgcgatcag aaacaggatc acttcgctcg atgcggccga gccgaggacg
      841 gcgagcgtca gccggtctcg cgcgaacggc agcaagatga acagcagcgc gaggtagacg
      901 tagacgcgaa acagcggcgt tcgcccgacg gcgtgcatgc ctcggcgcaa cacgtcctgc
      961 agcttgcccg cgtgcgcatc gtgatcggcc atcgtcgcgc tgccgtacgg atcttggatg
     1021 tcggtgaacc agttgtagac cggcgacgaa tcgtctccgc cgaagccgag caggcgcgtg
     1081 aacatcttcc agcgataggt caggtactcc tcgcgatgcg tcagcacgat cttcttccac
     1141 gcgcgcacca tcgcgttgcg ctcggcttcg tcgcgcggga ccgcaaagag gccgtacgcg
     1201 gtgttccaca gcgtcggtac gtactcggct tcctcgtcgc cgcggcgcgc gaactcgtga
     1261 aagtcgctgt cgggccagag acgcaggcct tcgaacgtct cgcgcagctc ggcgtcgggg
     1321 atcgtcggcg acgtgtagcg aagcgtcgcc gccatgtcac acatcgcgat gctgtccgtc
     1381 cacagatagc gatgctggtc ggtgagcgca ttcgacacga cgcgcgctga catcgtgagc
     1441 ccgaaccacg cgacgagcgc gatcgcgtag cgcttccacc accgatagtc cgggttccag
     1501 acaaagcaga ggaacacgat cggcagcgtg atcgtgagcg cgttgtggcg catcgccgtg
     1561 ccgaccgcga gcatgacgag gcccgagacg cggacccacc gctgcttctg gaggatgagc
     1621 ccggtgccaa gcaggatgaa cgcggtcatc tgcgagtcct tccagatcac cgccatgaag
     1681 gcagcgaccg gcggaaacca cagcaggaac acggctgcga acgccgccgc gttgggcttc
     1741 atgcgctcgc gaaagatcag atacatgccc gcgagaaaca cgacgctctg gatgatcagc
     1801 atcccgagcg gccccgcgat catcgcgtcg acatggcgcc acatcgctgc catcgccggc
     1861 gggtggccgt cgctgaaaat gccccagcgc gcttcgtcga gctggtagac ggagtcgtag
     1921 ctcatgtacc cggggaacgc gtacagcatg aacccgagcc aaccggcgag caggatgagc
     1981 cgcggcctga gcgccatcgg cggcgactat acttggatcg ctcgcgtgat atagctcgct
     2041 gagcgtgcgg gtgctgatca tcgtgccggc cttcaacgag gaagaggccc ttggtggact
     2101 tctggccgag ctgcgcgcgc tgccagctcc tgcaacggat gtcacgctcg agaccgtcgt
     2161 cgtcgatgac ggctcgtcgg atcgcacggc ggacgttgca cgctccggcg gcgctcgcgt
     2221 gctgcggctg tgcggcaacc tcggcatcgg tggcgcggtg caatcgggct tgcggctcgc
     2281 gcatcgcgag cgcttcgata tggcgatcca gctcgacggt gatggccagc acccgcctga
     2341 tcagctcgcg ccgctgatcg cctccgcacg caccgcctcg ccgcccgatc tcgtgatcgg
     2401 cacgcgctac tccgacaaga acaacttccg ctcgacgctg ctgcgacgcc tcggcagctg
     2461 gtggctgcgc gtgatcctgc gagtagtcac gcggacgagg ctctccgacc cg
//
LOCUS       DATLBZ010000032        25874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2993348, whole genome shotgun sequence.
ACCESSION   DATLBZ010000032 DATLBZ010000000
VERSION     DATLBZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 25874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25874
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2993348"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /gene="pheS"
                     /locus_tag="VMZ53_01500"
     CDS             <1..834
                     /gene="pheS"
                     /locus_tag="VMZ53_01500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HUS27144.1"
                     /translation="ANKVKEKIEQMTEASLKRLTNAAKQADLSRRVDVTLPARPVGGG
                     HLHLLTQVRHEAVAVFAELGFIVAEGPQIETDWHTFEALAIPKDHPARDMQDTFYVSE
                     EIVLRTHTSPVQVRTMLTQPPPIRIVAPGHVYRRDDDPTHSPMFTQIEGLAVDEGISF
                     ADLKGTLLHFVKRFFMKDLGIRLRPSYFPFVEPGAEVDMQCSFCGGSGCRICKQTGWV
                     EIGGAGMVDPDVFTNCHVDPEKYTGFAFGMGLERMAMLRHGVNDIKYYYEGDVRFLEQ
                     F"
     gene            837..3281
                     /gene="pheT"
                     /locus_tag="VMZ53_01505"
     CDS             837..3281
                     /gene="pheT"
                     /locus_tag="VMZ53_01505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HUS27145.1"
                     /translation="MKILWSWLLEMCDLDNVPSVEEGASALTRGGLEIEGMTNLGAAF
                     RGVVVAEVVGKKPHPQADKLTLVDVITERGGSATQVVCGAPNVPAPGRKVLWAQVGAE
                     LPGKDGKPITLSAKAVKGIESPGMLCAEDELGLSEDHAGIVILDEDDKTALGAPAQKA
                     LGIDDWLLELNAPANRGDLLGHIGVARELVAMLKGKLVWPDADLSAYSAGGKASIELA
                     IASGDECPRYVARLIEGLAVKASPRRLAQRLRAVGVRPISNLVDVTNYVMFELGQPLH
                     AFDAAKLSTGIVGVSPAGDGEKFTTLDGIDRTLVKTDLVIRDGDRGIALAGVMGGMDT
                     EVTDGTTRVLLESASFKPIMVRRTARRLGLHSEASSRFERGVDPELAAFASARASRLL
                     CMYGGGKVVGDAVDAYPGKRETPAIKVRLPRVQLLTGVPLRADVCCDALERLSFKVTG
                     TGDALQVTPPSVRADVTREVDVIEEVLRITGYEQVPSSLPIMRQAPPMRPADRADCAR
                     RALCAAGASEAITYGFQSADRCGSLNVAATDRRAQPIAIRNPMSAEQAVMRTSLLPNL
                     LAAVTRNQSYGRPDIALFEVGSVFLRKGEAVTERPLHELADEPTIAAGVLAGARADQI
                     GQGRAWDVFDAKSLALEAIRAVAGDVRVDARPTSSIPYLHPGVGGELIVDRDVVGCFG
                     EVHPDVREKLGVTGAVFAFEVELGKLRLAEPAQMKPIARFPGSQRDVSLLLSEDIPAS
                     RVVEVIEASHEPLLHRIRILEEYRDTSRLGDGMKSMLWSIAYRSPERTLTDAEVDRAH
                     ETIVARLVENLPAQRR"
     gene            3385..4593
                     /locus_tag="VMZ53_01510"
     CDS             3385..4593
                     /locus_tag="VMZ53_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27146.1"
                     /translation="MRAFIGLALSGLLLLTGCPGGGGDDGVGGACGTGFDPDTGTVGD
                     FGATAAAQKVEAMLHASADLYAAASTTEMETLAACTAMATDLGIPAGELQPASGELAV
                     TKACTRVKTEIDAIIKTLPVGVALGISVTPASCTVDLNVAASCAAECDASITGTAMVE
                     CKGELHGSCSASCSGKCSVMGNASCTGTCSGTCSGTCSGKCYGTCSVPCAVQDSMGNC
                     AGTCAGTCMGSCDVGCSGTCMGTCTSTVTGSCSGECYGSCDASWMAECNGEANVMANA
                     ECKAACDARASAKATCDPPTVTIVAMHVSDSTKDAKLQALITTLKTNYPKLIRVQQRV
                     QFSLAPAATSFAVSVQGASTSLASAGVQAAACMVAAVDAVVDASTRINASVMVTVSVS
                     ASVSASGTGG"
     gene            4842..5141
                     /locus_tag="VMZ53_01515"
     CDS             4842..5141
                     /locus_tag="VMZ53_01515"
                     /inference="COORDINATES: protein motif:HMM:NF001401.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HUS27147.1"
                     /translation="MARMTKADIIESVYEKVGGFSKKEAAEIVETVFNVVKETLERGE
                     KIKISGFGNFVVRDKKDRMGRDPQRAVPILIPARRVLTFKPSQVLKNILNGLPPV"
     gene            5201..5274
                     /locus_tag="VMZ53_01520"
     tRNA            5201..5274
                     /locus_tag="VMZ53_01520"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5235..5237,aa:Pro,seq:ggg)
     gene            5315..6079
                     /gene="surE"
                     /locus_tag="VMZ53_01525"
     CDS             5315..6079
                     /gene="surE"
                     /locus_tag="VMZ53_01525"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HUS27148.1"
                     /translation="MPRPLVLCSNDDGIHSPHLEALAAKIEAFADVLVVAPERQRSAA
                     SHAITLHKPLRLTEVRPGRYSLSGTPVDCVYLGVLKLCDRKPAVVVSGVNDGYNLGSD
                     VFYSGTVAAAVEGALRGAAGIACSLAPRAKDVDHAIDFAAKVVKSAVEEPIPAGTVLN
                     VNMPGKGMYEYQWTTLGRRLYEDDVAERRDPRGRPYYWVGGGPAGHDEVEGTDCVAIS
                     RGWNSITPMHLDLTDRGRLAKPPWEVEGFRVMVGPG"
     gene            6089..6457
                     /locus_tag="VMZ53_01530"
     CDS             6089..6457
                     /locus_tag="VMZ53_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27149.1"
                     /translation="MKRWLVFVAVLACACPAKKGTGPTTGSQGSGSDVAVVTPPTNAK
                     TCDEVKARVEGMYRAEAQAKEPKRVDEATADNTAMAMKDCATDPAKYVPCLAKAPTVA
                     ELEKSCLIPIDDEGTEGERK"
     gene            6484..6870
                     /locus_tag="VMZ53_01535"
     CDS             6484..6870
                     /locus_tag="VMZ53_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27150.1"
                     /translation="MKNLAFVLLVACGPKQPTTPSNTTHEIPPDNRTEIEKRRDGACD
                     TLGPRITECALEDAKAQYEAGKVSKSQFEQDTAPGVLKKNTEEFVKDCRKSDYSSRQI
                     RVLEVCQKEETECGPLLACLDNLNKQ"
     gene            6875..7945
                     /locus_tag="VMZ53_01540"
     CDS             6875..7945
                     /locus_tag="VMZ53_01540"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HUS27151.1"
                     /translation="MTKHWIVAAVLIAGCSKGKKTEETSTGSATPQAAKPQRMPLPAM
                     PALELPDDPKRKEKIELGHNLFFDKRLSGKGDLACYDCHKNEDGNGGHDPIAIGSGGV
                     KQTRHAPTLWNVGYWKNAGYWDGRAPTLEENTKGAWGGGNMGAGKDNLDKKAAEIAAI
                     PGYKKLFEDAFGKTEIKADQVAQALSEYMRTFVCNDTAYDKFAAGEKSALSDQQQRGL
                     DVFLGKGMCITCHSPPFFSTAMNLEGGAYFNVGVGTDVAEDQVDVGRMKVSNQPVDWA
                     AFKPPSLRNITKTPPYFHNGSAAKLEDAVKIMATGGIKNKNLTPIMADRALTPAEMGD
                     LVSFLGGLECQGKLDAPAALPK"
     gene            complement(7946..9205)
                     /locus_tag="VMZ53_01545"
     CDS             complement(7946..9205)
                     /locus_tag="VMZ53_01545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27152.1"
                     /translation="MQTVGGRYALDGPLGEGGMGQVYRARHLQLGKAFALKIIAPAFA
                     GDNAARQRFNQEAKLASEITHPNIVSVVDFGEDDQYGAYMVMELVEGDPLVSPGTMPM
                     SVKRAIDVLGQVADALDHIHKRGIIHGDVKADNIMLTAEQQGASGTRRRRVVRLLDFG
                     LARRPDHGEEETGVSGSPHYLAPERAAGGPPSVASDIYALGVLGYLMFTGTLPFDGSV
                     VEVLMAQIHNTPEPMNERRKEELDAALEALILRAMAKDPAQRHGSAAAFRYELNTVMD
                     MLDMGRRRPRGSGAMPMENAREVSIQQAFERSRIPQAIVSVEGFVVSCNRAFNKLVNQ
                     DDKGCEGLSLTDTTLHAFVPGLLRALRTVHVDGKPQERKARVYRSRDRPPLELTLWLS
                     PLPIPGHEIHMFVRVEEVDPRREDTSG"
     gene            9301..10263
                     /locus_tag="VMZ53_01550"
     CDS             9301..10263
                     /locus_tag="VMZ53_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27153.1"
                     /translation="MVRIWAVVLAIAIPTSAFAETEEQTEPAKFPDGLRVMASDLTIL
                     RLNPVGLETRARIGVQKKLYPSPKPITENNFFFAGFYPKLNPASAQLGIGGELQPASI
                     FNVRVLGEVQKYFGTFGYLQSFASANANYSDHTLADMKDQGAAATVKHFSVQPMLQLK
                     LGKIALRSLMMFDYWDVPGALAYEPTFDTLLPDKGWSVATDTDVLFVPGNGLAAGIRH
                     SWVKPMYSGAHFADAADQAAYDGKNSHHRVGFFGAYTFKDVGPSRFNKPTAILIVSFY
                     VKHKYRAGEPSTFDPGHDADDYRTRAFPYLIAGFAFESDFIDVR"
     gene            complement(10302..10376)
                     /locus_tag="VMZ53_01555"
     tRNA            complement(10302..10376)
                     /locus_tag="VMZ53_01555"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10339..10341),aa:Arg,seq:cct)
     gene            complement(10463..12205)
                     /locus_tag="VMZ53_01560"
     CDS             complement(10463..12205)
                     /locus_tag="VMZ53_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27154.1"
                     /translation="MAVAVDRAMGRQFLVVVGFFCACGGSSDTPPTPDAPVSPDIDAA
                     VDAPPPPPLMFGIDTSPAPSETYTQALATVHAAGATFVPQAIDWSMLEAGPDDTFGSA
                     IIDAKKWDVTGPFAADKPAGVVRYTSSAASGSDHLHARYALATGSISARAHAKLNTPG
                     NTQATVLLRFGTAPAAGNRCTDPADQFAAVVLLASGAAAAATCQNGVFATHGTQPATA
                     AATIQIRRTTTQIVFEVGSAQIAALNLSALPAAFAGTARLDVFAGNDAGAATVELSTL
                     HVTGAVTWDNPTDRFVGVDAAFDVPALLDLIHGPAHTKLVIILRTINTVTTELPSDLE
                     EVSSATGLVDMSRADIKQRYHAAIDYLIAHMPSVEIVGFSVGNEIDGYLGSNATAWAQ
                     VASFANDAIPYAKSKLPNAIVGITVTADGMIAHPTEAAAVNAEADAVFLTYYPLAPDF
                     TAKDPSVVPTDIARYLAATTKPVFFIESGYPSAGPSTACPTCTGSEAKQAAFFHAMFV
                     ATDTYPTTRLRGFTVIWMTDVPDATVQGWKDYYGISAPTFLAYLATLGVRTQSGAPKQ
                     AWTMIQSDAHARGL"
     gene            complement(12217..12609)
                     /locus_tag="VMZ53_01565"
     CDS             complement(12217..12609)
                     /locus_tag="VMZ53_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HUS27155.1"
                     /translation="MRIASCTCGQLKASCDGDPIRVSMCHCLACQRRTGCVFSSTSRW
                     PADRVTTAGTATEYVLVGDEGSAATFRFCPTCGATVYFTNDQMPGMIAVPVGAFADPT
                     FPPPQVSVYGVRRHPWTVMPGLVIEELD"
     gene            complement(12628..13497)
                     /locus_tag="VMZ53_01570"
     CDS             complement(12628..13497)
                     /locus_tag="VMZ53_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27156.1"
                     /translation="MGAAPASLLVAAFGIVAATAMPARADECTGNTPGVGKFALCFDP
                     GTRISITAGTDGFGAGIAVRHIVHFADEPDLVWRLEHVALDGVHAPNTEEFDALLYRG
                     RYTRHARDGHIVIPLGTPKKVFLPFDIGGVVEAGALTVRDERPARLRIVKVAGILDFA
                     RSRTFRKRLAFGPVARWDVDVATKPWDLGEHIVSPFSEAMANLHYETTNGRFVADLRV
                     EAGMAWRSTTGWEPTAEAEASIERIMLAINDRPISLVLGARYQTETEEAIAKVGARIV
                     LFDHRDPRVQRID"
     gene            complement(13470..14648)
                     /locus_tag="VMZ53_01575"
     CDS             complement(13470..14648)
                     /locus_tag="VMZ53_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27157.1"
                     /translation="MRPSIAALAVLGIGLPAIAVADDRYPMMDHEQPVVCARDTEGKI
                     WRIQCNAKTKVCLYAANEELSVDGDRVKTLERVRECDTSVIFDRPKLEAEGFTMLPGR
                     ADAPWGWMRDERGRVFQINFDLRRRLYFGVGWSPQKILDNLSDPTETRRSKMDFGLFV
                     FEHNDGRNRHRIRLLEGEVRVEPFSAEVVVAHYDMSRRFFDPLLRITTFVGEPQRHDL
                     HLNLGLWTEAGGLEIHPTPFGHSSIWKHATAEGTLDLWQSAHMESFARLRTGFGLEGQ
                     KTDGQEYRSALTAASAFELDWVVDPAGFHNIKLEIAHEMPRYFVPVTSKGQRLAQRSR
                     ARLSYEAIILAINDQPLTFQLAAGGEKRNDIMGVPDRWAFVMDAGLRFSLWAPPRPRS
                     "
     gene            complement(14814..15470)
                     /locus_tag="VMZ53_01580"
     CDS             complement(14814..15470)
                     /locus_tag="VMZ53_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27158.1"
                     /translation="MRAVWLFAIVIAGCTSESQLVLKPLPPLAPPVPPLIELVDGPLG
                     FSPGEHLIWEVQARGMAIGRLELDVADDAITSKFATNRVVSAFAKVDHELITLLDQGA
                     PVSSSERLDFDGELRQTTVNLSHSKLHSLHTALGLIRAWAHDGAHPGFASVAFMDQRF
                     KLQLEQPATDGDLLRVDAHITGDDTSIGITMWLDAEHRPVRIEIRANDERVTAELIAS
                     "
     gene            complement(15558..16379)
                     /locus_tag="VMZ53_01585"
     CDS             complement(15558..16379)
                     /locus_tag="VMZ53_01585"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HUS27159.1"
                     /translation="MRFSHKAIGAIAALMLATPFVGCAGDTASDGSALSGKNGGGGAT
                     DDDCTLTQGYWKNHADAWPVSSLKLGSVTYTKAELIAILKTPVKGNGLIALAHQLIAV
                     KLNIAAGASDVEIKASVTAADALIGSLVCPPKGNGELATNKTSSLVGKLDDYNNGKTG
                     PGHCGDNGGGGGSCEQGDDDHDDDHGDDDKGDDHDDDDGQCTTEPVCGNGIVEAGEQC
                     DDGNTLPGDGCCDLCEIEVPVCGNGIKELGEECDDGNLVNGDGCSTACVCECPNH"
     gene            16499..17380
                     /locus_tag="VMZ53_01590"
     CDS             16499..17380
                     /locus_tag="VMZ53_01590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUS27160.1"
                     /translation="MAISRTAEYVALLRALETTERRREPLFRDPYASRFLTGSRAAWH
                     TLLKVPGLRPMIERYSDTRGPGLRTSTIAQTAFIDDFVRKEVKRGIRQLVLLGAGFDV
                     RAHRIPELVETGSEVFEVDRPDMQEVKRKRLEGAPDLKKNVRYVHADLLSEHLPVKLA
                     DRGWEADHRSIFVWEGVTNYLDEPSVAAILDTVGRTKDGSVILFTYIHRGILDGSTKF
                     FGAEHLLSNVEKLGEPWKFGMLPEEVGSYVAQFGLKLEQDVGSDEYRKKYLGAADPGY
                     SFYRLAVARVVPSIARS"
     gene            complement(17377..17541)
                     /locus_tag="VMZ53_01595"
     CDS             complement(17377..17541)
                     /locus_tag="VMZ53_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27161.1"
                     /translation="MAEDAEGEASLGYRAIGRAAREARESGEGELQDDIRIVMNVLRS
                     HAKAPKRKKR"
     gene            17593..18309
                     /locus_tag="VMZ53_01600"
     CDS             17593..18309
                     /locus_tag="VMZ53_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27162.1"
                     /translation="MRLLLALVAMAGCGSVNDMPTKMDAAPQGDGHIDAVNTCTVHDQ
                     LASCGAQCAVCSTPSSDRMVATCDGTACGTACVGGAPRCTDMSCSRILFNFESDTQGT
                     VLVSPSGLAVNRRTHNGSMALAFDFTSNLANTQLEFHVPVCVTGTVNQSAKTLKADVY
                     FEGGADAGEQYYIQGSVPTPTAGNYLGTQGAAHNVTVAYSSAMSASAGSAAFNDITFR
                     IGSFGGAFTGTVWVDNIRIE"
     gene            complement(18310..19236)
                     /locus_tag="VMZ53_01605"
     CDS             complement(18310..19236)
                     /locus_tag="VMZ53_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HUS27163.1"
                     /translation="MNLEECLPPELLPAAFTRIAIGESGAGVYRVESAGTTYVLKIAP
                     DRQTTDIWRANVEIQRVAATAGVAPQIIHVDELRRAVLSEHIVDRGFPMRLMTPTTRV
                     AAITEMAGTLRRLHDAPVPANALAIRPREALREVRGRLDGFPLPAFATAVIERVLTET
                     PPTPGPAVLSHNDVNPSNIAFDGERLVLLDWDAAGANDAYYDLATVALFFRFDDATCL
                     SLISSHDGESVTALPARFVYLRRLVAAFCGTMMTHLARQAGHAGATDESLESAPALAD
                     IHARMRDGTLKLSTSEGRWQFGLALLKTATML"
     gene            complement(19251..20105)
                     /locus_tag="VMZ53_01610"
     CDS             complement(19251..20105)
                     /locus_tag="VMZ53_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HUS27164.1"
                     /translation="MRTVLAIIVVAAMAGSAIASPTRLPEVAQLESPSVVILDASTKR
                     ELFAKHADDPRAIASMTKIFAALVVRKHKLDLEAWTEITDEDAKTAEGGASTRLLRGE
                     SFRNVDLLHAMLLVSDNRVPTALARSVQLSPRELLDEMNAFAQDLGLEHTKFVDVTGI
                     NDNQSTAREMAIVLGLALKDPVLAKIMRTRYARITSKSETLTIDYKSTVLPLWTKGLK
                     VRGGKTGTTDAAGHCMIIGAKIAGRDVLMAFLGGESESSRFTDFAHAVHWLEHAEVEP
                     AHVATDAP"
     gene            20137..20883
                     /locus_tag="VMZ53_01615"
     CDS             20137..20883
                     /locus_tag="VMZ53_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27165.1"
                     /translation="MRWVLLALVFVVGCKKKEEAPAEEPLTGPPPTGHSVTKVGEEGS
                     AGGSATAPAGSGSTTATAGSSTTASPAGPVDAGTADASRFGGNGKPPYRDDTGHIHGP
                     GGPIFMGRGPDCTDKIDHCLRDGVWFAVGTIRPGSIYRATPAFEFEGKWWDFRETEVE
                     CEQLFKTRVAKPEDLIPGNELIWLVEEHQDFQWLNSEGDALVSSRWEAGVIEAGPAGS
                     DKFRVKGWQWAVRVDSARAIIEKKKCPGAS"
     gene            20914..21501
                     /locus_tag="VMZ53_01620"
     CDS             20914..21501
                     /locus_tag="VMZ53_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27166.1"
                     /translation="MEAEVLERGDVSFLFRPAVQAADALVTELAVQSFFMVLSSERGF
                     HRRVRIGRKRMPAAGQRLWARVERVGSMQRVLADIMEEEVYSTKTRGERYQPAARPIG
                     QGCYAFVRHGDHIHLAYRIENFELDAPDEVRPPDAASHVVLFEKVPRGRATWTTVGEP
                     TMLDEQGAELVLVGDEEEPERELGIAVLPAAVAVG"
     gene            21612..22727
                     /locus_tag="VMZ53_01625"
     CDS             21612..22727
                     /locus_tag="VMZ53_01625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS27167.1"
                     /translation="MSFEFEKALESVAAVAAQNAAAADRGEFPQATIDALRAAGLLGL
                     VSATSVGGQGQGLAQAAAVVERLARECASTAMIVCMHYAGTAVIEQHGPEDVRKAIAA
                     GKHLTTLAFSEPGSRSQFWASLNTATADGNDVRITGKKSFVTSAHKADSYVWSSKPVS
                     GSELSTLWLVPRTTQGLRVDGAFDGLGLRGNDSAPVTAEGVKLPQSARLGADGAGFGI
                     MMGAVLPWFNVMSANVSAGIMESLVERSVKHVAGTTFEHASSTLAELPTVRAFIARMR
                     IKTDQTKGLNFDTLAAIGGNRADATLRVLECKAAAGEAATEVGDLAMRVCGGAAFRKD
                     VGVERAFRDARAAVVMAPTTDALYDFIGKAVCGLPLF"
     gene            22736..23551
                     /locus_tag="VMZ53_01630"
     CDS             22736..23551
                     /locus_tag="VMZ53_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUS27168.1"
                     /translation="MPNTLVLGAVAYDAKVVPIWDGFIAWFAKRNFAFDYVLYSNYER
                     QVDAHLRGDFHVAWNSPLAWIEAERVAKKRGVAAEAIAMRDTDRDLKSVVLVREDSPV
                     RSLADLAGKRVGVGAADSPQATLIPLLSFADANVEVQVVRHDVLVGKHGDHIGGERDA
                     VKALLAGTVDAACVIDGNRVAFAREGLAPTRVIHATGAYDHCNMTVLGTPIPEVARFR
                     ELLLEMSYDDPEARPLLDLEGLKQWVPGRTSGYALLERACDRFGAIESWLAAR"
     gene            23548..24705
                     /locus_tag="VMZ53_01635"
     CDS             23548..24705
                     /locus_tag="VMZ53_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27169.1"
                     /translation="MSVVELADLGLDEGGHLLLKRALVANPRIIVRGTAAALAFDLPA
                     WCRALGHHCELTSEGFQITRSPHDRWRGAERAGGPDEVVESAPTRWGLAARGALVEAG
                     APELDLALSTKAEIWADEAARLYAQAAAAQWDPATAIPWDAPITHPDEVEDAVVQVMT
                     YLIENETAALLVPARFLGRIHPHFREVMQLLAIQTADEARHIEVFTRRAQLRRSALGL
                     STAGGQASLATLFDEPDFAIASFLLSVLGEGSFLSLLWFLRDHAPDPCTREVARLAAQ
                     DEARHVAFGLAHLQRHVSLDATLRGRLAAAIERRHGALQSTAGLNAEVFDALVLLAAG
                     DWSPASLRRGHAAVVELAADMDKGRRQRLARLGFDADQAASLSALHTRNFM"
     gene            complement(24698..25135)
                     /locus_tag="VMZ53_01640"
     CDS             complement(24698..25135)
                     /locus_tag="VMZ53_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HUS27170.1"
                     /translation="MSKAFTKEDEGTPPPPMRRLGAPVPEQNFVTPGGLRAAKAELDD
                     LARSGADPDRLRELTEHLATAQPLEPEDRKAVGLGAAVTIEDEDGKRTTYRLVGAIEA
                     DTKKGWLSWQTPIANALWGLRVGDSAALPKGEVTIVAIEYAST"
     gene            25322..25699
                     /gene="speD"
                     /locus_tag="VMZ53_01645"
     CDS             25322..25699
                     /gene="speD"
                     /locus_tag="VMZ53_01645"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HUS27171.1"
                     /translation="MTSLGRHLLCEYHGCDREILDTPDRIRGVMLAAVNRSGATPIHD
                     FVHSFTPHGVTGIVVIAESHFAIHTWPEYGFAAVDLFTCGDTVDPYAAFELLASSLKA
                     ARHSVVEMRRGLLPGPAPHKPGA"
BASE COUNT         4451 a         8357 c         8532 g         4534 t
ORIGIN      
        1 gcgaacaagg tcaaagagaa gatcgagcag atgaccgagg cgtcgctcaa gcgcctcacg
       61 aatgccgcga agcaggcaga cctgtcgcga cgcgtcgacg tcacgttgcc ggcgcgcccc
      121 gtcggtggcg ggcatctgca cttgctcacg caggtgcggc acgaagcggt ggccgtgttc
      181 gcggagctcg gcttcatcgt cgccgaaggc ccgcagatcg agaccgactg gcacacgttc
      241 gaggcgctcg cgatcccgaa ggatcatccc gcgcgcgaca tgcaggacac gttctacgtg
      301 tctgaagaga tcgtgctgcg tacgcatacg agccccgtgc aggtccgcac gatgctcacg
      361 caaccgccgc cgatccggat cgtcgcgccc ggtcacgtct atcgtcgcga cgacgatccg
      421 acgcactcgc cgatgttcac gcagatcgaa ggcctcgccg tcgacgaggg catctcgttc
      481 gcggacctca agggcacgct tctgcatttc gtgaagcgct tcttcatgaa ggatctcggc
      541 attcgtctgc ggccgagcta cttcccgttc gtcgagcccg gcgccgaggt cgacatgcag
      601 tgcagcttct gcggaggctc gggctgccgc atctgcaagc agaccggctg ggtcgagatc
      661 ggtggcgcgg gcatggtcga tcccgatgtc ttcacgaact gccacgtcga tcccgagaag
      721 tacacgggct tcgcgttcgg catgggcctc gagcgcatgg cgatgcttcg ccatggcgtg
      781 aacgacatca agtactacta cgaaggcgac gttcgcttcc tggagcagtt ctagccatga
      841 agatcctatg gagctggctg ctcgagatgt gcgacctcga caacgtgcct tccgtcgagg
      901 agggcgcgag cgcgctcacg cgcggtggtc tcgagatcga aggcatgacg aatctcggcg
      961 cagcgtttcg cggcgtcgtc gtcgcggaag ttgtcggcaa gaagccgcat ccgcaagccg
     1021 ataagctcac gctcgtcgac gtgatcaccg agcgcggtgg cagcgcgaca caagtcgtct
     1081 gcggcgcgcc gaacgttcct gcgccgggcc gcaaagtact gtgggcgcag gtcggcgcgg
     1141 agctgcccgg caaggacggc aagccgatca cgctgagcgc gaaagcggtg aagggcatcg
     1201 agtcgcctgg catgctgtgc gcggaggacg agcttggcct ctccgaagac cacgccggca
     1261 tcgtcatcct cgacgaggac gacaagaccg cgctcggcgc gcccgcgcag aaggcgctcg
     1321 gcatcgacga ctggttgctc gagctcaacg cgcctgcgaa tcgcggcgac ttgctcggcc
     1381 atatcggcgt cgcgcgcgag ctcgtcgcga tgttgaaggg caagctcgtg tggcccgatg
     1441 cggatctgtc cgcgtactcg gcgggcggaa aggcgagcat cgagctcgcg atcgcgagcg
     1501 gcgatgaatg cccgcggtac gtcgcgcggt tgatcgaagg cctcgccgtg aaggcgtcac
     1561 cgcggcggct cgcgcagcgg cttcgtgcgg tcggcgtgcg tccgatttcc aacctcgtcg
     1621 acgtgacgaa ctacgtcatg ttcgagctcg gccagccact tcacgcattc gatgcggcga
     1681 agctctcgac gggcatcgtg ggcgtgtcgc ccgcgggcga tggcgagaaa ttcacgacgc
     1741 tcgacggtat cgatcgcacg ctcgtcaaga cggacctcgt gatccgcgac ggtgaccgcg
     1801 gcatcgcgct cgccggcgtc atgggcggca tggataccga agtgaccgac ggtacgacgc
     1861 gcgtgctcct cgagagcgcg agcttcaagc cgatcatggt gcggcgcacg gcacgccgtc
     1921 tcggcctgca ctcggaagca tcgtctcggt tcgagcgcgg cgtcgatccc gagctcgcgg
     1981 cgttcgcatc ggcgcgcgca tcgcggctgc tctgcatgta cggcggcggc aaggtcgtcg
     2041 gcgacgcagt ggacgcatat cccggcaagc gcgagacgcc ggcaatcaag gtgcgcctcc
     2101 cgcgcgtgca gctgctcacc ggcgtgcctc tgcgcgccga cgtctgttgc gacgcgctcg
     2161 agcgcctcag cttcaaggtc accggcacgg gcgacgcgct gcaggtcacg ccaccgtcgg
     2221 tgcgcgccga cgtcacgcgc gaagtcgacg tcatcgagga agtcctgcgc atcaccggct
     2281 acgagcaggt gccgtcgtcg ctgccgatca tgcggcaagc gccgccgatg cggcccgcgg
     2341 atcgcgcgga ttgcgctcga cgcgcgctat gcgccgcggg tgcgtccgag gcgatcacgt
     2401 acggcttcca atcagcggac cgttgcggct cgctcaacgt cgccgccacc gatcgccgcg
     2461 cgcaaccgat cgcgattcgc aatccaatga gcgccgagca ggctgtgatg cgcacgtcgc
     2521 tgctgccgaa cctgctcgcc gcggtcacgc gcaatcagag ctacggccgg ccggacatcg
     2581 cgctgttcga agtcggcagc gtgttcttgc gcaaaggcga agcggtcacc gagcgtccgt
     2641 tgcacgagct cgccgacgag ccgacgatcg ccgcgggcgt gctcgcaggt gcgcgcgcag
     2701 accaaatcgg ccagggcagg gcgtgggacg tgttcgacgc caaatcgctc gcgctcgaag
     2761 cgatccgcgc cgttgccggt gacgtccgcg tcgatgcacg tccgacgtcg tcgatcccgt
     2821 atctgcatcc cggcgtcggc ggcgagctca tcgtcgaccg cgacgtcgtc ggctgcttcg
     2881 gcgaagttca tcccgacgtg cgcgagaagc tcggcgtcac cggcgccgtg ttcgcattcg
     2941 aagtcgagct cggcaagctg cgcctcgcgg agcccgcgca gatgaagccc atcgcccggt
     3001 tcccgggaag ccagcgcgac gtgtccctgc tgctctccga ggacattcca gcgtcgcgcg
     3061 tcgtcgaggt gatcgaagcc tcgcacgagc cgctgcttca ccggattcga atcctcgagg
     3121 agtatcgcga tacgtcgagg ctcggtgacg gcatgaagag catgctgtgg tcgatcgcct
     3181 accgctcgcc ggagcgtacg ctcacggacg ccgaagtcga tcgcgcacac gagacgatcg
     3241 tcgcgcgcct cgtcgagaac ctccccgcgc agcgccgctg accgatcact gcacaccgcc
     3301 gctcagcgca ctcgatctgg caaaccgctc gcaaggcgcc cgttagattg aattgcgggt
     3361 gcattgcctg cgtatgatgt ggccatgagg gcatttattg gtcttgcttt gagtggattg
     3421 ctgctgctca cgggctgtcc cggcggcggt ggtgacgatg gtgtcggcgg tgcatgcgga
     3481 accggatttg atccggacac cggcaccgtc ggtgatttcg gcgcgacagc cgctgcgcaa
     3541 aaggtcgagg cgatgctgca cgcatctgcc gacctctacg ccgccgcatc aacgaccgag
     3601 atggaaacgc tcgccgcgtg tacagcgatg gcgacggacc tcggcattcc ggcgggcgag
     3661 ctgcagccgg cgtcgggcga gctcgcagtg acgaaggcat gcacgcgcgt gaagacggag
     3721 atcgacgcga tcatcaagac gctgcccgtc ggtgttgcgc tcggtatcag cgttacgccg
     3781 gcatcgtgca cggtcgatct caacgtcgcc gcgagctgcg cggcggagtg cgacgcatcg
     3841 atcaccggca ctgcaatggt cgaatgcaaa ggcgagctcc acgggtcgtg cagcgcgagc
     3901 tgcagcggca agtgctcggt gatgggcaac gcgagctgca cgggaacgtg tagcggcacc
     3961 tgctcgggaa cgtgctcggg taagtgctac ggcacgtgct cggttccgtg tgccgtgcag
     4021 gacagcatgg gcaactgcgc cggaacatgc gcgggaacct gcatgggcag ctgcgacgtg
     4081 gggtgctcgg gcacctgcat gggcacgtgc acgagcacgg tcaccggttc gtgcagcggt
     4141 gagtgctacg gcagctgcga cgcgagctgg atggcggagt gcaacggcga ggcgaacgtc
     4201 atggcgaacg ccgaatgcaa agcggcgtgc gacgcgcgcg cgagcgcgaa ggcgacatgc
     4261 gatccgccga cggtcacgat cgtcgcgatg cacgtcagcg actcgacgaa ggacgcgaag
     4321 ctccaagcgc tgatcaccac gctgaagacg aactatccga agctgatccg ggtgcagcag
     4381 cgcgtgcaat tctcgctcgc tcccgcagcg acgagcttcg cggtctccgt gcagggcgcg
     4441 tcgacctcgc tcgcgagcgc cggcgtgcaa gcggcagcct gcatggttgc ggcggtcgat
     4501 gcggtcgtcg atgcttcgac gcgcatcaac gcgtcggtga tggtgaccgt cagcgtgtcg
     4561 gcatcggtca gcgcgtcggg cactggcggc tagcgcaccg ctgacatcgt tgtccgcacc
     4621 gcgtcggggc gtctcgaaat tctgagtcgc cccgacgtgc tttcggttgt ttggcgacgt
     4681 gtccgcgtgg aaagcggcgt tcgaatggaa ctcccgtatc ggcctcgcgt tctagtctaa
     4741 gtacctgaaa gcaggccgca gaaactggcg tgccagggat ctcgaaggct ttcagtcgct
     4801 tgcgttacgc atgttccgta aacccgtgtt acgtatgtct tatggcgaga atgaccaagg
     4861 cggacatcat cgagtcggtc tacgaaaaag taggcggttt ctcgaagaag gaagccgcgg
     4921 agatcgtcga gaccgtcttc aatgtcgtca aggagacgct cgagcgcggc gagaagatca
     4981 agatctcggg tttcggtaac ttcgtcgtac gcgacaagaa agaccgaatg ggccgcgatc
     5041 cgcagcgcgc ggtgccgatc ctgattccgg cgcgtcgcgt gctgacgttc aagccctcgc
     5101 aggttctgaa gaacatcctc aatggtttgc cgcccgtctg attgctcccg cgccgttcat
     5161 tgacggcggg agagcgatct agtagaccct ccttcccacc cgaggcgtag cgcagcctgg
     5221 tagcgcacca gactgggggt ctgggggtcg caggttcaaa tcctgtcgcc tcgaccggga
     5281 acggacggca accccagccg tccacctttt ttagatgccc cgtcctctcg tcctctgcag
     5341 taacgacgac ggcatccact cgcctcatct cgaggcgctc gcggcgaaga tcgaagcgtt
     5401 cgccgacgtg ctcgtggtcg cgccagagcg gcagcgttcc gcggcgtcgc atgcgatcac
     5461 gctgcacaag ccgctgcggc tgaccgaagt tcggcctggg cgctactcgc tctcgggaac
     5521 cccggtcgac tgcgtgtacc tcggcgtctt gaagctgtgc gatcgcaaac ccgcggtcgt
     5581 cgtgtcgggt gtcaacgatg gctacaacct cggcagcgat gtcttctact cgggcaccgt
     5641 cgcggcggcc gtcgagggag cgctgcgcgg agcggcgggc attgcgtgct cgcttgcgcc
     5701 tcgcgcgaaa gatgtcgacc acgcgatcga ctttgcagcg aaggtcgtca agtccgccgt
     5761 cgaggagccg atccccgcgg gcacggtgct caacgtgaac atgccgggca aaggcatgta
     5821 cgagtaccag tggacgacgc tcgggcgacg gctctacgaa gatgatgtcg ccgagcgccg
     5881 cgatccgcgc ggacggcctt actactgggt cggcggtgga ccggcgggcc acgatgaagt
     5941 cgaaggcacg gactgcgtcg cgatctcgcg cggctggaac agcatcacgc ccatgcatct
     6001 cgatctcacc gatcgcggcc ggctcgcgaa gccaccgtgg gaggtcgaag gctttcgcgt
     6061 gatggtcggc ccggggtaag gtccgcgcat gaagcgctgg ctggtcttcg tcgcggtgtt
     6121 ggcgtgtgca tgcccggcga agaaggggac gggacctacg accggctcgc aggggagtgg
     6181 gagcgacgtc gcggtggtga cgccgcccac gaacgcgaag acgtgcgacg aagtgaaggc
     6241 gcgcgtcgaa gggatgtatc gcgccgaagc gcaggcgaag gagccgaagc gcgtcgacga
     6301 agcgaccgcc gacaacacgg cgatggcaat gaaagactgc gcgacggatc cggcgaagta
     6361 cgtgccatgc cttgcgaagg cgccgaccgt cgccgagctc gagaaatcct gcctgattcc
     6421 aatcgatgac gaaggtaccg aaggcgagcg caaatgacgt ccgctgcgcg tgtctccatg
     6481 gtcatgaaga acctcgcatt tgtgctcttg gtcgcatgcg gccccaagca gccgacaacc
     6541 ccgagcaaca ccacgcacga gattccgccg gataaccgca ccgagatcga gaagcgtcgc
     6601 gatggcgcat gcgacacgct cggcccgcgg attaccgagt gcgcgctcga agacgcgaag
     6661 gctcagtacg aagccggcaa agtcagcaag tcgcagttcg aacaagacac cgcgccgggc
     6721 gtcttgaaga agaacaccga agagttcgtg aaggactgcc gcaaatccga ttactcgagc
     6781 cggcagatcc gcgttctcga ggtctgccag aaggaagaga cggagtgcgg cccgttgctc
     6841 gcgtgtctcg acaacctcaa caagcagtga gctcatgacc aagcactgga tcgtcgccgc
     6901 agttctgatc gccggttgca gcaaaggcaa gaagaccgaa gagacgagca cggggagcgc
     6961 gacgccgcag gcagcaaagc cgcagcgcat gccgcttccc gcgatgccgg cgctcgagct
     7021 ccccgacgat ccgaagcgca aagagaagat cgagctcggt cacaacctgt tcttcgacaa
     7081 gcggctctcc ggcaaaggcg acctcgcttg ctacgactgc cacaagaatg aagacggcaa
     7141 cggcggtcac gatccgatcg cgatcggctc gggcggcgtg aagcagaccc gtcacgcacc
     7201 gacgctgtgg aacgtcggct actggaagaa tgcgggctac tgggatggcc gcgcgccgac
     7261 gctcgaggag aacacgaagg gcgcgtgggg cggcggcaac atgggcgcgg gcaaagacaa
     7321 cctcgacaag aaggccgccg agattgcggc gatccctggc tacaagaagc tgttcgagga
     7381 cgcgttcggc aagaccgaga tcaaagctga tcaggtcgcg caggcgttga gcgagtacat
     7441 gcgaacgttc gtctgcaacg acaccgcata cgacaagttc gccgccggcg agaagtcagc
     7501 gctcagcgac cagcagcagc gcggcctcga cgtgttcctc ggcaaaggca tgtgcatcac
     7561 gtgtcactcg ccgccgttct tctcgacggc gatgaacctg gaaggtggcg cgtactttaa
     7621 cgtcggcgtc ggaacggacg tcgccgaaga tcaagtcgac gtcggccgca tgaaggtctc
     7681 gaaccaaccg gtcgattggg ctgcgttcaa gccgccgagc ctgcgcaaca tcacgaagac
     7741 tccgccgtac ttccacaacg gctccgccgc gaagctcgag gacgcggtca agatcatggc
     7801 gaccggcggc atcaagaaca agaacctgac gccgatcatg gcggaccgcg ctctcacgcc
     7861 ggcggagatg ggcgacctcg tctcgttcct cggtggcctc gagtgccaag gcaagctcga
     7921 cgcgcccgcg gcgctgccga agtagttacc cgctcgtatc ttcgcggcgc ggatcgactt
     7981 cttcgacgcg cacgaacatg tggatctcgt gccccggaat cggcagcgga gatagccaca
     8041 gcgtcagctc gagtggcggc cgatcacggc ttcgatagac gcgagccttg cgctcttgtg
     8101 gcttgccatc gacgtgcacc gtgcgcaacg cgcgcagcaa tcccggcacg aacgcgtgca
     8161 gcgtcgtatc ggtgagcgat aggccttcgc aacccttgtc gtcctgattg acgagcttgt
     8221 tgaacgcgcg attgcacgac acgacgaagc cctcgacaga cacgatcgcc tgcgggatgc
     8281 gagaccgctc gaacgcctgt tggatcgaaa cctcgcgcgc gttctccatc ggcatcgcgc
     8341 cgctgccgcg cgggcgcctg cgacccatgt cgagcatgtc catcacggtg ttcagctcgt
     8401 agcgaaacgc agccgcactg ccgtgacgct gtgccggatc cttcgccatc gcgcgcaaga
     8461 tgagcgcctc gagcgcggca tcgagctctt ctttgcgccg ctcgttcatc ggctcgggcg
     8521 tgttgtggat ctgcgccatc aacacttcga cgacgctgcc atcaaacgga agcgtgccgg
     8581 tgaacatcaa gtaacccagt acgccgagcg cataaatatc gctcgccacg ctcggcggcc
     8641 cacccgctgc gcgctccggc gccaagtaat gcgggctgcc gctgacgccc gtctcttcct
     8701 ctccatgatc cggccgccgc gcgagcccga aatcgaggag tctcacgacg cgccggcgcc
     8761 gcgtaccgct cgctccctgc tgctccgccg tcagcatgat gttgtcggct ttcacgtcgc
     8821 cgtgaatgat gccgcgcttg tgaatgtgat cgagtgcgtc ggcgacttgc ccgagcacat
     8881 cgatcgcacg cttcacgctc atcggcatcg tgccgggcga gacgagtgga tcgccctcga
     8941 cgagctccat caccatgtac gcgccgtatt gatcgtcttc accgaagtcg acgaccgaca
     9001 cgatgttcgg gtgcgtaatc tcgctcgcga gcttcgcctc ttgattgaag cgctgccgag
     9061 cagcgttatc gccggcaaat gcaggcgcga tgatcttcag cgcgaatgct ttaccgagct
     9121 ggaggtgccg agcccgatag acctgaccca tcccgccttc gcccagtggc ccatcgaggg
     9181 cataacggcc gcccaccgtt tgcatgagaa ataaatataa agccagctca cacctgggtg
     9241 ccacaacctg gtgcactcac tcgctggtac gacctctctc cacatgcgta tgctgccggg
     9301 atggtgcgta tctgggcggt ggtactggcg atcgcgattc caacctcggc attcgcggag
     9361 accgaagagc aaacggagcc ggcgaagttt cccgacggtc tgcgcgtcat ggcgtcggat
     9421 ctgacgatct tgcgcctcaa ccccgtcggc ctcgagacgc gcgcgcgcat cggcgtccag
     9481 aagaagctgt acccgtcgcc caaaccgatc acggagaaca acttcttctt cgcgggcttc
     9541 taccccaagc tcaatcccgc gagtgcgcag ctcggcatcg gcggcgagct ccagcccgca
     9601 tcgatcttca acgtgcgcgt gctcggcgag gtgcagaaat acttcggcac gttcggctat
     9661 ctccaatcgt ttgcgtccgc gaacgcgaac tactccgacc acacgctcgc cgacatgaaa
     9721 gaccagggcg cagccgcgac cgtcaaacac ttcagcgtgc agccaatgct gcagctcaag
     9781 ctcgggaaga tcgcgctgcg ctcgctgatg atgttcgact actgggacgt gccgggcgct
     9841 ctcgcgtacg agccaacgtt cgacacgctg cttccggaca aaggctggtc ggtggcgacc
     9901 gataccgatg ttctgttcgt tccgggcaac gggctcgcgg ccggaatccg gcacagctgg
     9961 gtcaagccga tgtactcggg cgcgcacttc gccgatgcgg ccgaccaagc ggcgtacgac
    10021 ggcaagaaca gtcaccatcg cgtcggcttc ttcggtgcgt acacgttcaa ggacgtcgga
    10081 ccgtcccgct tcaacaagcc aaccgcgatc ttgatcgtct cgttctacgt gaagcacaag
    10141 taccgcgccg gcgagccgtc gacgtttgac ccagggcacg acgccgacga ctaccgcact
    10201 cgcgcattcc cgtacttgat cgctggcttt gcgttcgagt cggacttcat cgacgttcgc
    10261 tagaacgcgg gcaatcaccc tgcaaagtgt ccatgtggtt gtgatcccag caggaatcga
    10321 acctgcggcc taatctttag gaaagatttg ctctaatcca gctgagctat gggaccgaaa
    10381 cgaacgcacg gtacttagca caggagcgcc tccagtgttt ggatctttgc ggtggcctcc
    10441 ttgtgggggc ctctgcgctg ctctacagcc cgcgagcgtg ggcgtcggat tggatcatcg
    10501 tccacgcttg cttcggcgca cctgattgag tccggacgcc gagcgtcgcg aggtacgcga
    10561 ggaacgtcgg cgcggagatg ccgtagtagt ccttccagcc ttggacggtc gcgtccggca
    10621 cgtccgtcat ccagatcacg gtgaagccgc gcagccgcgt cgtcggatac gtgtcggtcg
    10681 ccacgaacat cgcgtggaag aatgcagcct gctttgcttc ggagcccgtg cacgtcgggc
    10741 atgccgttga tggtccggca ctcggatagc cggactcgat gaagaagacc ggtttcgtcg
    10801 tcgcggcgag gtagcgcgcg atgtccgtgg ggacgacgct cggatctttc gcggtgaagt
    10861 cgggcgcgag cggatagtac gtcaggaaga ccgcatcggc ttctgcgttc accgcggccg
    10921 cttcggtcgg atgcgcgatc atgccgtcgg cggtcacggt gatgccgacg atcgcgttcg
    10981 ggagcttcga cttggcgtac gggatggcat cgttcgcgaa cgacgcgacc tgtgcccatg
    11041 ccgttgcgtt cgagccgagg tatccgtcga tctcgttgcc gacggagaaa ccgacgattt
    11101 ccacactcgg catgtgcgcg atcaggtagt cgattgccgc gtggtagcgc tgtttgatgt
    11161 ccgcgcgcga catgtcgacg agccccgttg ccgagctgac ctcttcgaga tccgatggca
    11221 gctcggtcgt caccgtgttg atcgtccgca ggatgatgac gagcttcgtg tgcgcgggtc
    11281 cgtggatcag atcgagcagt gccggaacgt cgaacgcggc atcgacgccg acaaagcgat
    11341 cggtcgggtt gtcccacgtg acggcgcccg tgacatgaag cgtcgacagc tcgacggttg
    11401 ctgcgcctgc gtcgttgcct gcgaatacgt cgaggcgtgc ggtgccggcg aatgctgctg
    11461 gcaaggctga gagattcagt gccgcgattt gtgcgctgcc tacttcgaat acgatctgcg
    11521 tcgtcgtgcg gcggatctga atcgtggcgg ccgcagtcgc cggttgcgtt ccgtgcgttg
    11581 cgaacacgcc gttctggcac gtcgccgctg cggccgcacc actggcgagc agcaccacgg
    11641 cagcaaattg atcggccgga tccgtgcagc gattgccagc ggcgggcgcg gtgccaaagc
    11701 ggagcaggac cgtcgcctgc gtgttgcctg gcgtgttgag cttcgcgtgt gcgcgtgccg
    11761 agatcgatcc ggtcgccagt gcgtaacgcg catgcaggtg gtcgctgccg ctcgcagcgg
    11821 acgaggtgta gcgcacgacg ccagcgggct tgtccgctgc gaacggtcca gtgacgtccc
    11881 acttcttcgc gtcgatgatc gcgctgccga acgtgtcgtc gggcccggct tcgagcatcg
    11941 accaatcgat tgcctgcggc acgaacgtgg cacctgccgc gtgaacggta gcgagtgcct
    12001 gcgtgtaggt ctcgctcggt gcgggcgacg tgtcgatacc gaacatcagc ggcggaggcg
    12061 gtggcgcgtc gaccgctgcg tcgatatccg gcgacacggg cgcatcgggt gtcggcggag
    12121 tgtcggaact tccaccgcat gcgcagaaaa acccgacaac aacgaggaac tgcctgccca
    12181 ttgctcgatc cacagcaacc gccatgcccg ctcgccctag tcgagctcct cgatcacgag
    12241 gccgggcatc acggtccatg ggtggcgtcg tacgccgtag accgagacct gcggcggcgg
    12301 gaatgtcgga tctgcgaacg cgccgaccgg caccgcgatc attcctggca tctgatcatt
    12361 cgtgaagtag accgtcgcgc cgcacgtcgg gcagaaccgg aatgtcgcgg cgctgccttc
    12421 gtcgccgacg agcacgtact ccgtcgcggt gcccgccgtc gtcacgcggt cagccggcca
    12481 gcgcgatgtc gacgagaaca cgcagcccgt gcggcgttgg cacgcgaggc agtggcacat
    12541 cgagacccgg atcggatctc cgtcgcagct ggctttcagt tgcccgcagg tgcatgaagc
    12601 gattcgcatc gagcgcgagc atagcgatta gtcgatgcgt tgtacgcgcg ggtcacgatg
    12661 atcgaagagg acgatccgtg cgccgacctt tgcgatcgct tcttcggttt cggtttgata
    12721 gcgcgcgccg agcacgagcg agatcgggcg atcgttgatc gcgagcatga tgcgttcgat
    12781 cgacgcttcg gcttcggccg tgggttccca gcctgtcgtc gagcgccacg ccatgccagc
    12841 ctcgacgcgg aggtcggcga cgaagcggcc gttggtcgtc tcgtagtgga ggttcgccat
    12901 cgcttcggag aacggcgaca cgatgtgctc gccgaggtcc cagggctttg tcgcgacgtc
    12961 gacgtcccac cgcgcgaccg ggccgaatgc gaggcgcttg cggaatgtgc gcgagcgcgc
    13021 gaagtcgagg atgccggcca ccttgacgat gcgaaggcgc gcgggacgtt cgtcgcgcac
    13081 cgtgagggcg cccgcttcga cgacgccgcc gatatcgaac gggaggaaca ccttcttcgg
    13141 cgtgccgagt gggatgacaa tgtgaccgtc gcgcgcgtga cgggtgtagc ggccgcggta
    13201 gagcagggcg tcgaactctt cggtgttggg agcgtgcacg ccgtcgagcg cgacgtgctc
    13261 gaggcgccat acgagatccg gctcgtctgc gaagtgaacg atgtgacgga cggcgatgcc
    13321 ggcgccgaag ccgtcggttc ccgcggtgat cgagatgcgc gtgcctggat cgaagcagag
    13381 cgcgaatttg ccgacacccg gcgtgttgcc ggtgcactcg tccgcgcgtg cgggcatcgc
    13441 cgttgccgcg acgatcccga acgcagcgac taggagcgag gccggggcgg cgcccacagg
    13501 ctgaacctca ggcccgcgtc catcacgaat gcccagcggt caggaacgcc catgatgtcg
    13561 tttcgcttct cgcctccggc agcgagctgg aacgtgagag gttggtcgtt gatcgcgagg
    13621 atgatcgcct cgtacgagag tcgtgcacgc gagcgttgag ccaggcgttg gcctttgctc
    13681 gtaacgggca cgaagtagcg cggcatctcg tgcgcgatct cgagcttgat gttgtggaag
    13741 ccggccgggt cgacgaccca atcgagctcg aacgcggatg cggccgtgag cgccgagcgg
    13801 tactcctgcc cgtcggtctt ctggccttcg aggccaaagc cggtgcgcag gcgtgcgaac
    13861 gactccatgt gcgccgactg ccagagatcg agcgtgcctt cggcggtcgc gtgcttccag
    13921 atcgacgagt ggccgaacgg cgtcgggtgg atttcgaggc cgcccgcttc cgtccagagg
    13981 ccgaggttca agtgcaggtc gtggcgctgc ggctcgccga cgaacgtcgt gatgcgcagc
    14041 agaggatcga agaagcgccg cgacatgtcg tagtgcgcga cgacgacctc ggcggaaaac
    14101 ggctcgacgc gaacctcgcc ttcgagcaag cggatgcgat ggcggttgcg cccgtcattg
    14161 tgctcgaaca cgaagaggcc gaagtccatc ttcgagcgcc tcgtctccgt cggatcgctc
    14221 aggttgtcga ggatcttttg cggtgaccag ccgacgccga agtaaagccg ccgtctcaga
    14281 tcgaagttga tctggaacac gcgcccgcgt tcgtcgcgca tccagcccca cggtgcgtcc
    14341 gcgcggccgg gcagcatcgt gaagccctct gcctcgagct tcggtcgatc gaagatcacc
    14401 gacgtgtcgc attcgcgaac acgctcgagc gtcttcactc gatcgccgtc gacggatagc
    14461 tcctcgtttg cggcgtacaa gcagactttc gtctttgcgt tgcactggat tcgccaaatt
    14521 ttgccttcgg tgtctctcgc gcagaccacg ggttgttcgt gatccatcat cggatagcga
    14581 tcgtcggcga cggcgatcgc gggcagacct atcccgagca ccgctagagc ggcgattgac
    14641 ggcctcattg ctcgcgtcgc ggctgcaaag gtcgaaccag gcaacttgca aacgcgccgt
    14701 gcgatcgctt cgtggcgcgt cagtgagttg tggtcgcgtc acttcttgcc acgtgtgccg
    14761 atcgagaaca cgtgttgtgg gcacgcaaca cttcgtaaca atcacgcgcg cgatcagctc
    14821 gcgatcaact ctgcggtcac gcgttcgtcg ttcgcgcgaa tctcgatacg caccggacga
    14881 tgctctgcat cgagccacat cgtgattccg atcgacgtgt cgtcaccggt gatgtgcgcg
    14941 tctacgcgca acagatcgcc atcggttgca ggctgttcga gctgcagctt gaagcgttga
    15001 tccatgaacg cgacgctcgc gaatcctgga tgtgcgccgt catgagccca cgctcgaatg
    15061 aggccgagcg cggtgtgcag cgagtgcagc ttcgagtgcg agaggttgac cgtcgtttgc
    15121 cgcaattctc cgtcgaagtc gaggcgttca ctcgagctca ctggcgcgcc ttgatcgagc
    15181 agcgtgatga gctcgtgatc gaccttcgcg aacgcgctga ccacgcgatt tgtcgcgaac
    15241 ttgctcgtga tcgcatcgtc tgcgacgtcg agctcgagcc ggccgattgc catgccgcgc
    15301 gcttggactt cccagatcag atgctcaccc ggagaaaacc ccagcgggcc gtcgacgagc
    15361 tcgatgagtg gcggcaccgg cggcgcgagc ggaggcagcg gcttgagcac gagctgcgac
    15421 tcgctagtac atccggcgat cacgatcgcg aacagccaga cggcgcgcat gaggccgcgt
    15481 gcgctgcaat tgtcgaaccg aaactgacaa caccgtgtca aacgacacgg cgccatcagc
    15541 tcctccgtta actgtgatca gtgattcggg cactcgcaga cgcaggccgt cgagcagccg
    15601 tcgccattca cgagattgcc gtcgtcacac tcttcgccga gctccttgat cccattgcca
    15661 cacaccggaa cttcgatctc gcagaggtcg cagcagccat cgcccggcag cgtgttgccg
    15721 tcgtcgcact gctcgccagc ttcaacgatg ccgttgccgc acaccggctc ggtggtgcac
    15781 tgaccgtcgt cgtcgtcatg gtcatcgccc ttgtcgtcgt cgccatgatc gtcgtcgtgg
    15841 tcgtcgtcgc cttgctcaca cgagccgcca ccgccgccgt tgtcaccgca gtgacccggg
    15901 ccggtcttgc cgttgttgta gtcgtcgagc ttgccaacga gtgacgacgt cttgttcgtc
    15961 gcgagctcgc cattgccctt cggcgggcag acaaggctgc cgatcagtgc atcggccgcg
    16021 gtgaccgagg ctttgatctc gacatccgac gcgccggccg cgatgttgag cttcaccgca
    16081 atcagctgat gcgctagcgc gatgaggcca ttgcccttca cgggcgtctt caagatcgcg
    16141 atcagctcgg ccttcgtgta cgtgaccgag ccgagcttca gagaggagac cggccacgca
    16201 tccgcgtggt tcttccagta tccctgcgtg agcgtgcagt cgtcgtcggt cgcaccgccg
    16261 ccgccattct tccccgagag cgccgaccca tccgaggccg tgtcgcccgc gcatccgacg
    16321 aatggcgtcg cgagcatgag tgccgcgatc gctccgattg ctttgtgact gaatcgcatt
    16381 gagaggctcc tgagttttca gaggcctcca ttgctagtcg gataccaaac acttcgcggc
    16441 gcatttgtat gcagttacac gtttccgtcc gtgcgcccat gagacagagt ggtagccgat
    16501 ggcgatcagc cgcacggccg agtacgtcgc gctgctccgc gcgctcgaga ccaccgagcg
    16561 ccgtcgcgaa ccgctgtttc gcgatccata cgcctcacgc ttcctcacgg gcagtcgcgc
    16621 ggcatggcac acgctgctca aagtcccggg gctgcgcccg atgatcgagc gctactccga
    16681 cacgcgcggc ccgggccttc gaacctcgac gatcgcccag acggcgttca tcgacgactt
    16741 cgtgcgcaag gaagtgaagc gcggcattcg ccaactcgtg ctgctcggcg cgggcttcga
    16801 cgtccgtgcg caccgcatcc ccgagctcgt cgaaacggga agtgaagtct tcgaggtcga
    16861 tcgccctgac atgcaggagg tcaaacggaa gcggctcgaa ggcgcaccgg atctcaagaa
    16921 gaacgtgcgc tacgtgcatg cggatctgct gagcgagcat ctcccggtca agctcgcgga
    16981 ccgcggctgg gaggccgacc accggtcgat cttcgtgtgg gaaggcgtca cgaattatct
    17041 cgacgagcca tccgtcgcgg cgatcctcga taccgtcggc cgcacgaagg atggcagcgt
    17101 catcctcttc acgtacatcc atcgcggcat cctcgacggc tcgacgaagt tcttcggcgc
    17161 cgaacatctc ctctcgaacg tcgagaagct cggcgagccc tggaagttcg gcatgctccc
    17221 cgaagaagtc ggcagctatg tcgctcagtt cggtctcaag ctcgagcaag acgtcggctc
    17281 ggacgagtac cgcaagaaat acctaggcgc ggcggatccc ggatacagct tctatcggct
    17341 agctgtcgcc cgcgtcgtgc cgtcgatcgc gcgcagctaa cgcttcttgc gttttggcgc
    17401 ctttgcgtga gagcgcagga cattcatcac gatgcgaatg tcgtcttgga gctcgccctc
    17461 gccggattcg cgggcctctc gtgccgctcg tccgatcgcg cgatagccga ggctcgcctc
    17521 gccttcggca tcttctgcca cttggtatcg gtcgccatag tgaagcctag cgccctgcga
    17581 tacgatcgcc agatgaggct tctgctcgca ctcgtcgcaa tggccggatg cggttccgtc
    17641 aacgacatgc cgacgaagat ggatgcggcc ccgcagggcg atggacacat cgatgcggtg
    17701 aatacgtgca ccgtgcacga tcagctggcg agctgcggcg cgcagtgcgc cgtatgcagc
    17761 acgccatcga gcgatcgcat ggtcgcaacg tgcgatggca cggcgtgtgg caccgcgtgc
    17821 gtcggcggcg ctccgcgctg caccgatatg agctgctcgc ggatcctgtt caacttcgag
    17881 agcgatacgc agggcacggt gctcgtgtcg ccgagcggcc tcgcggtcaa ccggcgcacg
    17941 cacaacggca gcatggcgct cgcattcgac ttcacgtcga atctcgcgaa cacccagctc
    18001 gagttccacg tcccggtgtg cgtcacgggg actgttaatc agtcggcaaa gacgctgaaa
    18061 gcggacgtct acttcgaggg cggcgcggac gcgggagagc agtactacat ccaaggttcg
    18121 gtaccgacgc cgacagccgg caattacctc ggtacgcaag gtgcggcaca caacgtcacc
    18181 gttgcgtact cgtcggcgat gagcgcgtcc gcgggaagcg cagccttcaa cgacatcacg
    18241 ttccgcattg gctcatttgg tggagcgttt acgggcacgg tgtgggtcga caatattcgg
    18301 atcgagtagt cagagcatcg tcgcggtctt gagcagcgcg agaccgaact gccagcgccc
    18361 ctcactcgtc gagagcttga gcgtgccatc gcgcatgcga gcgtggatgt cagcgagcgc
    18421 cggcgcggat tcgagcgact cgtcggtcgc gccggcgtga cctgcttggc gagcgaggtg
    18481 agtcatcatc gtgccgcaga acgccgcgac gagccggcgc agatacacga agcgcgcggg
    18541 caacgcggtg acgctctcgc catcgtggct ggagatcagc gataggcacg tcgcatcgtc
    18601 gaagcggaag aacagcgcga cggtcgctag atcgtagtag gcgtcgttcg ctccggcggc
    18661 gtcccagtcg agcaggacga gccgttcgcc gtcgaacgcg atgttcgatg gattcacgtc
    18721 attgtgactg aggaccgcgg ggcctggcgt gggcggcgtc tcggtgagca cgcgctcgat
    18781 caccgcagtc gcgaatgccg gaagcgggaa tccgtcgagg cgaccccgaa cttcgcgaag
    18841 cgcttcgcgc ggtcgtatcg cgagtgcgtt tgcgggcacc ggtgcgtcgt ggagacggcg
    18901 gagtgtgccg gccatctcgg tgatcgcagc gacacgtgtc gtgggcgtca tcaggcgcat
    18961 cgggaatccg cgatcgacga tgtgctcgct cagcacggcg cggcggagct cgtcgacgtg
    19021 aatgatctgc ggggcgacac cggcggtagc cgcgacgcgt tggatctcga cgttggcgcg
    19081 ccagatatcg gtggtctggc ggtccggcgc aatcttcagc acgtacgtcg tgcccgccga
    19141 ttccacgcgg tagacgcccg cgcctgattc accgatcgcg atgcgcgtga atgcggcggg
    19201 gagcagctcg ggcggcaggc actcttcgag attcacgtgc ggagcatacg tcagggcgcg
    19261 tcggtcgcga cgtgcgctgg ctcgacctcc gcatgctcga gccaatgcac ggcatgcgcg
    19321 aagtccgtga acctcgatga ctcgctctcg ccgccgagga acgccatgag cacgtcgcgg
    19381 ccggcgatct tggcgccaat gatcatgcaa tggccggcgg cgtcggtcgt tcccgtctta
    19441 ccgccgcgga ccttcagccc cttcgtccac agcggcaaca ccgtcgactt gtaatcgatc
    19501 gtcagcgtct cgctcttcga cgtaatgcgc gcgtagcgag tgcgcatgat cttggcgagg
    19561 accggatctt tcagcgcgag gccgaggacg atcgccatct cgcgcgcggt cgattggttg
    19621 tcattgatcc ccgtgacgtc gacgaatttc gtgtgctcga ggccaaggtc ctgcgcgaac
    19681 gcgttcatct cgtcgaggag ctcgcgcggc gacagctgca cggagcgggc gagggcggtc
    19741 ggcacgcggt tgtcggagac gagcagcatc gcgtgcagta gatccacatt gcggaacgat
    19801 tcgccgcgca gcaaacgcgt cgaggcgcct ccctctgcag tcttcgcgtc ttcgtcggtg
    19861 atctcggtcc acgcttcgag atcgagcttg tgcttgcgca cgacgagcgc cgcgaaaatc
    19921 ttcgtcatcg acgcgatcgc gcgcggatcg tcggcgtgct tggcgaacag ctcgcgcttc
    19981 gtcgacgcgt cgaggatgac gacgctcggc gattcgagct gcgcgacctc ggggagccgc
    20041 gtggggctcg cgatagcaga cccggccatc gctgccacca caatgatcgc gagcaccgtg
    20101 cgcacgttgg accagtatag ctagtaagat gcagggatgc gctgggtgct gcttgcgttg
    20161 gtattcgtcg tcgggtgcaa gaagaaggaa gaagctccgg cagaggagcc gttgaccggg
    20221 ccgccgccga cgggacactc cgtcacgaag gtcggcgaag aaggctccgc tggcggatcg
    20281 gcgacggcgc ctgcggggtc ggggtcgacg acggccaccg cgggatcgag cacgaccgcg
    20341 tctcccgctg ggccggtcga tgcggggacg gccgacgcgt ctcgcttcgg tggcaacggc
    20401 aagccgccat atcgcgacga caccggtcac atccatggtc cgggcggtcc gatcttcatg
    20461 ggccgcggcc ccgattgcac cgacaagatc gatcactgcc tgcgcgacgg cgtgtggttc
    20521 gcggtcggaa cgatccggcc gggctcgatc tatcgcgcca cgcccgcgtt cgagttcgag
    20581 ggcaagtggt gggacttccg cgagacggag gtcgaatgcg agcagctgtt caagacgcgg
    20641 gtcgcgaagc ccgaagatct cattcccggc aacgagctga tctggctcgt cgaggaacac
    20701 caggacttcc agtggctgaa cagcgagggc gacgcgctcg tgtcgtcgcg ctgggaggcg
    20761 ggcgtgatcg aagcgggacc cgcgggctcc gacaagttcc gagtgaaggg ctggcagtgg
    20821 gcggtccgcg tcgacagcgc gcgcgccatc attgaaaaga agaagtgccc gggcgcgagt
    20881 tgacgtagcg cggaggcgcg tcacggttct cctatggagg ctgaggttct cgaacgagga
    20941 gacgtcagct tcttgtttcg gccggccgtg caggcggcgg atgcgctcgt caccgagctc
    21001 gcggtgcaga gcttcttcat ggtgctctcg agcgagcgtg gctttcatcg gcgcgtccgc
    21061 attggtcgca agcgcatgcc cgccgcgggc cagcggctgt gggcgcgagt cgagcgggtc
    21121 ggctcgatgc agcgcgtgct cgcagacatc atggaagaag aggtctactc gacgaagacg
    21181 cgcggagagc gctatcagcc ggcggcgcga cctatcgggc agggctgcta cgcattcgta
    21241 cgccatggcg atcacattca cttggcgtat cgcatcgaga acttcgagct cgatgcaccc
    21301 gacgaggtga ggccacccga cgcggcgagc cacgtcgtcc tgttcgagaa ggtgccgcgc
    21361 gggcgtgcga cgtggacaac cgtcggcgag ccgaccatgc tcgacgagca aggggcagag
    21421 ctcgtcctcg taggcgatga ggaagagccg gagcgggagc tgggcatcgc cgtgttgcct
    21481 gctgcggtcg ccgtaggcta gggtccacac atgttccgtc gttgaatcca cctgcggccc
    21541 gacgtggtcg ggtcacacag ctgcgacggg tagccgcgac tctctagcgg tgctcgccgc
    21601 tggagaagtc catgtcgttc gaattcgaaa aagctctcga gtctgtcgca gccgttgccg
    21661 cgcagaatgc cgcagccgcg gatcgcggcg agtttccgca ggcaaccatc gatgcgctgc
    21721 gcgccgctgg cctgctcggt ctcgtcagcg cgacgtccgt cggtggtcaa gggcagggcc
    21781 ttgcccaagc cgccgctgtc gtcgaacgcc tcgcgcgcga gtgtgcgtcg accgcgatga
    21841 tcgtctgcat gcactacgcg ggtaccgcgg tcatcgagca gcacggtccc gaggatgtgc
    21901 gcaaggcgat cgcagcgggc aagcacctca cgacgctcgc cttctccgag ccgggctcgc
    21961 gcagccagtt ctgggcgtcg ctcaatacgg caactgccga cggcaacgac gtccgcatca
    22021 ccggcaagaa gagcttcgtc acgtcggcgc acaaggccga cagctacgtg tggtcatcga
    22081 agccggtctc gggcagcgag ctctcgacgc tgtggctcgt cccgcggacg acgcagggcc
    22141 ttcgcgtcga cggagccttc gacggtctcg gtctgcgcgg caatgactcg gcgccggtca
    22201 cggcggaggg cgtgaagctt ccgcagtccg cacgcctcgg cgccgatggc gctggcttcg
    22261 gaatcatgat gggagccgtg ctgccgtggt tcaacgtgat gagcgcgaac gtctcggcgg
    22321 gcatcatgga gagcttggtc gagcgctccg tcaagcacgt cgcgggcacg acgttcgagc
    22381 acgcatcctc gacgctcgcc gagctgccga cggtgcgagc gttcatcgcg cgtatgcgca
    22441 tcaagacgga tcaaacgaag gggctcaact tcgacacgct cgcggccatc ggtggcaacc
    22501 gcgccgacgc gacgctccgc gtgctcgagt gcaaggccgc cgccggcgaa gccgcgaccg
    22561 aagtcggcga cctcgcgatg cgcgtgtgtg gcggcgcagc gttccgcaag gacgtcggcg
    22621 tcgagcgcgc attccgtgat gcacgtgcag cggtcgtgat ggcgccgacg acggacgcgc
    22681 tctacgattt cattggcaag gccgtctgcg gactgccgct cttctagagg acacgatgcc
    22741 caacacactc gtactcggcg cagttgctta cgacgcaaag gtcgttccga tttgggacgg
    22801 cttcatcgcg tggttcgcga agcgcaactt cgcattcgac tacgttctct actcgaacta
    22861 cgagcgtcag gtcgacgcgc acctgcgcgg tgattttcac gtcgcctgga actcgccgct
    22921 cgcgtggatc gaggccgagc gggtcgccaa gaagcgcggc gtggcggcgg aagccatcgc
    22981 gatgcgcgat accgatcgcg acctcaagag cgtcgtgctc gttcgcgagg acagccccgt
    23041 gcgctcgctc gcggacctcg ccggcaagcg agtgggcgtc ggcgctgctg actcgccaca
    23101 ggcgacgctc atcccgttgc tctcgtttgc cgacgcaaac gtcgaggtcc aggtcgttcg
    23161 ccatgacgtc ctcgtcggca agcacggcga tcacatcggc ggcgagcgcg acgctgtgaa
    23221 ggcgctgctc gcgggcaccg tcgacgcggc gtgtgtcatc gacggcaacc gcgtcgcatt
    23281 tgcgcgcgag ggcctcgcgc ccacccgcgt gatccacgcg accggcgcgt acgatcactg
    23341 caacatgacg gtgctcggca cgccgattcc ggaagtcgca cgctttcgcg agctcctgct
    23401 cgagatgtcg tacgacgatc cggaggctcg cccgctgctc gacctcgaag gcctcaagca
    23461 gtgggtgccc ggtcgcacgt cgggctacgc gctcctcgag cgagcatgcg atcgcttcgg
    23521 cgcgatcgag agttggctgg ccgcgcggtg agcgtcgtcg agctcgcaga cctcggcctc
    23581 gacgaaggcg gccacctgct gctcaagcga gcgctcgtcg cgaaccctcg catcatcgtg
    23641 cgcgggaccg ccgccgctct ggcgttcgat ctgccggcgt ggtgtcgcgc gctgggccac
    23701 cactgcgagc tcacgtccga gggcttccag atcacgcgat cgccgcacga ccgctggcgc
    23761 ggtgccgagc gggcaggtgg ccccgatgag gtcgtcgagt ctgcgccgac gcgctggggc
    23821 ctcgcggcgc gcggtgcgct cgtcgaagcg ggcgcgcccg agctcgacct cgcgctctcc
    23881 acgaaagccg aaatctgggc ggacgaagcc gcacgcctct acgcccaagc tgccgcagct
    23941 cagtgggacc cggccaccgc aatcccatgg gatgcgccga tcacgcatcc cgacgaagtc
    24001 gaggacgccg tcgtccaagt gatgacgtac cttatcgaga acgagaccgc cgcgctcctc
    24061 gttcccgctc gcttcctcgg gcgcatccac ccgcacttcc gcgaggtgat gcagctgctc
    24121 gcgatccaga ccgccgacga agcgcggcac atcgaggtct tcacgcggcg cgcgcaactc
    24181 cgccgctccg cgctcggcct gtccaccgcg ggcggccaag cctcgctcgc gacgctcttc
    24241 gacgagcctg acttcgccat cgcgtcgttc ctcctctccg tcctcggcga aggcagcttc
    24301 ctgtcgctgc tgtggttcct gcgcgatcac gcgcccgacc catgcacgcg cgaggtcgcg
    24361 cggctcgccg cacaagatga agcgcgccac gtcgcattcg gcctcgcgca cctccagcgt
    24421 cacgtctccc tcgacgccac gctgcgcggc cgcctcgccg ccgcaatcga acgccgccac
    24481 ggtgcgcttc agtccactgc cggcctcaac gccgaggtct tcgacgcgct cgtcctgctc
    24541 gccgcaggtg actggtcgcc ggcttcactg cgtcgcggtc acgccgctgt cgtcgagctt
    24601 gcagccgaca tggacaaagg ccgccgccag cgcctcgctc gcctcggctt cgacgccgac
    24661 caagccgcgt ccctctcggc gctccacacg cgcaacttca tgtagacgcg tactcgatcg
    24721 cgacgatcgt gacctcgccc ttcggcaagg ccgctgagtc gccgacgcgc agtccccaca
    24781 gcgcgttcgc gatcggggtc tgccacgaga gccagccctt cttcgtgtct gcttcgatcg
    24841 cgccgacgag gcggtaggtc gtgcgcttgc cgtcttcatc ttcgatcgtg actgccgcgc
    24901 cgagcccgac cgccttgcga tcttcaggct cgagcggttg cgcggttgcg agatgctcgg
    24961 tcagctcgcg gaggcgatcg ggatcggcgc ccgacctcgc tagatcgtcg agctcggcct
    25021 tcgccgcgcg cagcccgccc ggcgtcacga agttctgctc cggcacgggt gcgccgaggc
    25081 gccgcatggg cggcggcgga gtgccttcat cttctttcgt aaaggccttg ctcacacggt
    25141 cactgtaccg cgggcatgtc gcttgctctc tactagaagc ggaggttcac acatgtcgac
    25201 tacagcttgg attgttgtgc tcgttctgct cgcgttgctc ctcggtggcg gtggcttctt
    25261 ctatcgccgc tgagctttgt agacacaacg tgccttcatc gccgacagta cggcgatggg
    25321 catgacctca ctgggcagac atctgctctg cgagtatcac gggtgtgatc gggagatcct
    25381 cgacacaccc gatcgtattc gcggcgtgat gctcgccgcg gtgaaccgct cgggcgcgac
    25441 acccattcat gacttcgtgc attcctttac gccgcacggc gtaacgggaa tcgtcgtcat
    25501 tgccgaatcg cacttcgcga tccacacgtg gcccgaatac ggcttcgccg cggttgatct
    25561 cttcacgtgc ggcgacacgg tcgatccgta cgcggcgttc gagctgctcg cctcgtcgct
    25621 caaggcggcg cggcactcgg tcgtcgaaat gcgccgcggc cttctgcctg ggccagcgcc
    25681 gcacaagccg ggcgcctgat caggcgtaga gcgcacgcca gtacacgcct tcaccggcgt
    25741 gctcgacgtg cacttcctcc cacgtgacgc cggagtcgcc gatcgcttct ttcgcggcgc
    25801 gcgtcacgag gatctcgttg ccttttgcgg tgtcctcgcc gagcttgctc gcgacgttga
    25861 cctcgtggcc gaac
//
LOCUS       DATLBZ010000033         7357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8965307, whole genome shotgun sequence.
ACCESSION   DATLBZ010000033 DATLBZ010000000
VERSION     DATLBZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8965307"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VMZ53_01650"
     CDS             <1..196
                     /locus_tag="VMZ53_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27172.1"
                     /translation="YTFAGKPQKRTPTLDQKFSIPFESDGTTSTRTIKVTFSDAEHTD
                     TQEIKVTAGQTSEVACKLTP"
     gene            193..1140
                     /locus_tag="VMZ53_01655"
     CDS             193..1140
                     /locus_tag="VMZ53_01655"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS27173.1"
                     /translation="MKPVSVSGSRTSRYAGQTTLAIARIVVACAALLVSSIADATPRD
                     DLKAANKAFREGNYPDARDKYNGLLYPEIQLADSNDLVEAYVNLGVCRLETGDEGGAK
                     REFEKALQIDPNRQLDPLVITNKKAVALFDDTKTDIRVREEREKNARREAGEKERIRK
                     LRAGLIGVQGNTKAYIAVPFGIGQFQNGDRKKGAFFLGAELVTVSTSVGIFAYLLNKY
                     GIKSNAVPLEDGPRVRRLQQIEIGSGIAFYGLWIWGAVDAYRHYKPQVRVKLDESLLP
                     PELRETPPPKKPKTPKTSYHLVPMLTPDGGAGIGIGWEN"
     gene            1142..2950
                     /locus_tag="VMZ53_01660"
     CDS             1142..2950
                     /locus_tag="VMZ53_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS27174.1"
                     /translation="MPSLRLTAPGQPPTIYHLHKKITSLGSGPDNDIVLPDPLVQDGY
                     AIHFDGQTYTVFAPKKTEFVVNGKKRSKHKLTHDERLTIGSVDLRFAMVDEHAPVESE
                     AANTIADLDAYTKLFEFSDRLMHVRDLTELLDALMDAVIQITNADKGFLILLEGETLD
                     VKVARNLNKENIADAVSQLSDSIIAKVVRSRKPVIVSDAMSDDEFAGAKSVMHLKVSS
                     VICVPLLDRGRLLGIIYVGNDSIRDLFQEQMLRVLTVFASQAALIVSNALHMNELKHD
                     NKRLNDRLEQYRFGEIVGTSPPMQQVFRKVEKIAPTDISVLITGETGTGKELIAREIH
                     NRSPRTGKPFVTINCGAIPENLLESELFGHIKGAFTGAVANKQGKFQAADGGTLFLDE
                     IGEMPIELQVKLLRAIQEKVVYRVGDTRPETCDIRILAATNRDLEKEIAGGRFREDLY
                     YRLNVVNVELPPLRVRGDDVLVIARYLLSRYSREYDAKVKGFSPNAAVAIRKHNWPGN
                     IRELENRIKKAIVLCESTVIGPDDLGLTGDVLPQILTLADAKEKFQREYINEVLALNN
                     GNRTKTARDLGVDPRTVFRHLEKESEDGSEVPPDPV"
     gene            3036..3572
                     /locus_tag="VMZ53_01665"
     CDS             3036..3572
                     /locus_tag="VMZ53_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27175.1"
                     /translation="MARPLHGAGVLRAVVALGFAMFAGCVFPPSLSVDTTDAGANSAP
                     AIVSVRADLVEFPQFSTLVFEKGNGAGQLNVTLHDTDLDDTLYVKIFVNYNRPDPTPA
                     RSQCETAGKTVERTCSAPMQAVCTTADIGANPLPVMQVFVSDADVEPNIQPPFQGLPP
                     GRLSTSLTFFLRCQDPSL"
     gene            3569..5332
                     /locus_tag="VMZ53_01670"
     CDS             3569..5332
                     /locus_tag="VMZ53_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27176.1"
                     /translation="MRLALLLPLVAGCLEVPKGAGQECNYDSDCNTASGEVCDEGLCY
                     GNPPTGNYAGTLSAPIEREDLISTEIPLLSFPVNGHLPDLELEAPVTFSGRVEAYCPA
                     SATCPTTSIAAEVRLTRPSRFPGGPALRLSAQSKAGVPRGTDSFSILLPRTHEGDEPY
                     TVTIDGDGGGEKPAANGGTDPAELVPPKRLVLYATDNLEHQTYSLGGANPVVITGVLK
                     DALGGYLTSYRVVALGRWDTDAGTTEVSTVDYVTNGQYSITLSEGLVGPIELVAKSFD
                     PNVTAPELHVVGVLPSSQMRNIGQPTGLGSRVEVTIPIKGVSAEGPVEPVSGARVIIT
                     GRQEAAFPGAATALFTQEVITGDDGLAKFALLDGMQLQQSYRLRVIPPASSDLGVVFG
                     AELDLQKPAMVQLPKRVKLRGRVLDVTGQPLADVSVTARRSLRFLWSLDSDNQEFLDE
                     IPAATAITQEAGDFVVWVDPALADAWAFYDLSFKTPTGSSAPSWMIPDFQIPRLATQT
                     MVEVNDVVIPDGAYVRGKVVDPAGNPVEGSALRVFLLPTTDQLCYEVTHAPDNCSVEA
                     TVMGDGESDMQGIVRLDLPRP"
     gene            5616..>7357
                     /locus_tag="VMZ53_01675"
     CDS             5616..>7357
                     /locus_tag="VMZ53_01675"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS27177.1"
                     /translation="MTNDTTPTENVSADAAALVAAMQTETTEATSDTPVEPKPPSFDD
                     LGLHPDVKQALDDMGYFAPTPVQTAVFTPVSEGKNLLVQSRTGTGKTTAFGLPLVNKM
                     VPTHRQPQALILAPTRELALQVARELTNIGKHRGIVIEAVYGGAPIGKQITALRNGVH
                     IVVGTPGRVLDHIGRRTLDCKTISTFVLDECDEMLSMGFLEDIEKITSNLPDKHQTLL
                     FSATMPDEVTRYARRHMQEREQISLSRDSVSVSDIHHAYYIVSGIARSRDLLKILFAE
                     NPESAIIFCNMRDETTMVAKMLQKQGLDAEPLSSDLSQNDRERVMARMKAKNLRFLCA
                     TDVAARGIDISNLSHVINFSVPESPEVYVHRTGRTGRAGKKGTALSLVGPRELGNFRY
                     VRLQFGIKPEERLLPKDDIFVGKLKVPLPPVGVPQPPDPVQILIRGVPGTPSDLERQI
                     FEKLLQKSEGKRVLAALVADKLNALSTKTSRPRRAERPEGDAGAGEAGGGGDEGRRTG
                     RFDRPDRPRFGADGERGERPRFGDGERGDRGDRGDRPRFDRDRGDRPRFGGDVGGERG
                     ERPDRGDRPRFGADG"
BASE COUNT         1387 a         2421 c         2323 g         1226 t
ORIGIN      
        1 gtacacgttc gccggtaagc cgcagaagcg cacgccaacg ctcgatcaaa agttctcgat
       61 cccgttcgag agcgacggca cgacatcgac gcgaacgatc aaggtcacct tcagcgacgc
      121 ggaacatacc gacacgcagg agatcaaagt cacggcgggc cagacgtcgg aggtggcgtg
      181 caagctcacc ccgtgaagcc cgtgagcgtg tccggttctc ggacatcgcg ctatgctggt
      241 cagaccaccc tagcgatcgc gcggatcgtc gtcgcatgcg cagcgctctt ggtctcgagt
      301 atcgcggacg cgacaccacg cgatgacctc aaggccgcga acaaggcgtt tcgcgagggc
      361 aactaccccg acgcgcgcga caagtacaac gggctcctct accccgaaat ccagctcgcc
      421 gactcgaacg acctcgtcga agcgtacgtg aacctcggcg tgtgccgcct cgagacgggc
      481 gacgaaggcg gcgcgaagcg cgagttcgag aaggcgctgc agatcgatcc gaaccggcag
      541 ctcgatccgc tcgtcatcac gaacaagaag gcagtcgccc tgtttgacga cacgaagacc
      601 gacatccgcg tacgcgagga acgcgagaaa aatgcgcgac gcgaggccgg ggagaaggag
      661 cgcattcgca agctgcgcgc tggcctcatc ggcgtgcagg gcaacacgaa ggcgtacatc
      721 gcggtgccgt tcggcatcgg ccagttccag aacggcgaca ggaagaaggg cgcgttcttc
      781 ctcggcgcgg agcttgtgac cgtctcgacg agcgtcggaa tcttcgccta cctgctcaac
      841 aagtacggca tcaagtccaa cgctgttccg ctcgaggacg gtccgcgtgt acgccgcctg
      901 cagcagatcg agattggcag cggcatcgca ttctatgggc tgtggatctg gggcgccgtc
      961 gacgcgtatc gccattacaa gccgcaggtc cgcgtgaagc tcgacgagtc actcttgccg
     1021 cccgagctcc gcgagacgcc gccgccgaag aagccgaaga ctccgaagac gtcctatcac
     1081 ctcgtcccga tgctcacgcc cgatgggggc gccggtattg gaatcggttg ggagaattag
     1141 catgccgtcg cttcgcctta ctgcaccggg tcagccgccg accatctatc acctgcacaa
     1201 gaagatcacg tcgctcggct cggggcctga caacgacatc gtgttgccgg atccactcgt
     1261 gcaagacggc tacgcgattc acttcgacgg ccagacgtac acggtgttcg cgccgaagaa
     1321 gacggagttc gtcgtcaacg gcaagaagcg cagcaagcac aagctgacgc acgacgagcg
     1381 gctcacgatc ggcagcgtcg atctgcggtt tgcgatggtc gacgagcacg cgcccgtcga
     1441 gtccgaggcc gcgaacacga tcgcggatct cgacgcgtac acgaagctgt tcgagttctc
     1501 tgaccggctc atgcacgtcc gcgatctgac ggagctgctc gacgcgctga tggatgccgt
     1561 cattcagatt acgaacgccg acaagggctt cttgatcttg ctcgagggcg agacgctcga
     1621 cgtcaaggtc gcgcgcaact tgaacaagga gaacatcgcc gacgccgtca gccagctgtc
     1681 tgactcgatc atcgcgaaag tggttcgctc gcgtaagccc gtgatcgtca gcgacgcgat
     1741 gtccgacgac gagttcgccg gcgcgaagag cgtcatgcat ctcaaggtgt cgagcgtgat
     1801 ctgcgtgccc ctgctggatc gaggccggtt gctcggcatc atctacgtcg gcaacgattc
     1861 gattcgcgac ctgtttcagg aacagatgct gcgcgtgctg accgtgttcg cgtcgcaggc
     1921 ggcgctgatc gtctcgaacg cgctgcacat gaacgagctc aagcacgaca acaagcgctt
     1981 gaacgacagg ctcgagcagt atcggttcgg cgagatcgtc ggcacgtcgc cgccgatgca
     2041 gcaggtgttt cgcaaggtcg agaagatcgc gccgaccgac atcagcgtgc tgatcaccgg
     2101 cgagaccggc accggcaagg agctgatcgc gcgcgagatc cacaatcgct cgccgcgcac
     2161 cggcaagccg ttcgtcacga tcaactgcgg agcgattccc gagaacctgc tcgagagcga
     2221 gctgttcggt cacatcaagg gcgcgtttac cggcgccgtc gcgaacaagc agggcaagtt
     2281 ccaagcggcc gatggcggca cgctgttcct cgacgagatc ggcgagatgc cgatcgagct
     2341 gcaggtaaaa ttgctgcgcg cgatccagga gaaggtcgtc tatcgcgtcg gcgatacgcg
     2401 gccggagacg tgcgacatcc gcatcctcgc cgcgacgaac cgcgacctcg agaaagagat
     2461 cgccggcggg cggtttcgag aggacctcta ttaccggctc aacgtggtca acgtcgagct
     2521 gccgccgctg cgcgtgcgcg gcgatgacgt gctcgtgatc gcgcgctatc tgctgtcgcg
     2581 gtactcgcgc gaatacgacg cgaaggtcaa aggcttctcg cccaacgccg cggtcgcgat
     2641 tcgcaagcac aactggccag gcaacattcg cgagctcgag aaccgcatca agaaggcgat
     2701 cgtgctgtgc gaatcgaccg tgatcggccc cgatgatctc ggcctaaccg gcgacgtgct
     2761 gccgcagatc ctcacgctcg ccgacgcgaa ggagaagttc cagcgcgagt acatcaacga
     2821 ggttctcgca ctgaacaatg gcaaccgaac gaagacggcg cgcgatctcg gcgtcgatcc
     2881 acgcacggtg tttcgccacc tcgaaaagga atcggaggac ggcagcgagg tgccgcccga
     2941 cccggtgtga tttccgtcgc gaattcctcg aagagcccat gacgcgaatg tccgaaccaa
     3001 tcaaaccaac cgcccgcact gtcgagcagt tagcgatggc gcggccgctg catggtgcag
     3061 gtgtgctgcg cgctgtggtt gcccttggat ttgcgatgtt tgccggttgt gtattcccgc
     3121 cgtcgttgtc cgtcgatacg acggacgctg gagccaattc cgcgcccgca atcgtgagcg
     3181 ttcgcgctga cctcgtcgag ttcccgcagt tctcgacgct cgtgttcgag aaaggtaacg
     3241 gagccggtca gctgaacgta acgctccacg acaccgacct cgacgacacg ctctacgtga
     3301 agatcttcgt gaactacaac cgtcccgatc cgacgcctgc gcgcagccaa tgcgagaccg
     3361 cgggaaagac cgtcgagaga acgtgcagcg cgccgatgca agcggtctgt acgacggcgg
     3421 acatcggcgc gaacccgctg cccgtcatgc aggtgttcgt ctccgatgcg gacgtcgagc
     3481 cgaacatcca gccgccgttt caagggttgc cgccgggccg cctgtccacg tcgctgacgt
     3541 tcttcttgcg ctgccaggat ccatcgctgt gaggctcgcg ctccttctcc cgctcgttgc
     3601 tggctgcttg gaagttccca agggcgcggg acaggagtgc aactacgact cggactgcaa
     3661 caccgcctct ggcgaggtct gcgacgaggg gctttgctac ggcaatccgc cgaccggcaa
     3721 ctatgcaggc acgctgtcgg cgccgatcga gcgcgaggat ctgatctcga ccgagattcc
     3781 gctgctgtcg ttcccggtca atggccacct gcccgacctc gagctcgaag caccggtgac
     3841 gttctccgga cgcgtcgaag cgtactgccc cgcgtcggcc acatgcccga cgacatcgat
     3901 tgccgcggaa gtccggctga cgcgcccgtc gcggtttccc ggcggccctg cgcttcggct
     3961 ctccgcgcag tcgaaggcgg gcgtgccgcg aggcaccgac tcgttctcga tcctgttgcc
     4021 gcgtacgcac gaaggcgacg agccgtacac cgtgacgatc gacggcgatg gcggcggcga
     4081 gaaaccagcg gcgaacggcg ggactgatcc agcagagctc gtgccgccga agcgcctcgt
     4141 cctctacgcg actgacaacc tcgagcacca gacgtactcg ctcggcggcg cgaaccctgt
     4201 cgtcatcacc ggcgtgttga aagacgcgct cggtggctac ctcacgagct atcgcgtcgt
     4261 cgcgctcggc cgctgggata cggatgccgg aacgaccgag gtctcgaccg tcgactacgt
     4321 cacgaatggc caatactcga tcacgttgtc cgaggggctc gtcggtccga tcgagctcgt
     4381 cgcaaagtcg ttcgatccga acgtgacggc gcccgagctg cacgtcgtcg gcgtgctgcc
     4441 gagctcgcag atgcgcaaca tcggccagcc gacgggcctt ggcagtcgcg tcgaagtgac
     4501 gattccaatc aagggcgttt cggcagaagg tcccgtcgag ccggtcagcg gcgcgcgcgt
     4561 catcatcacc ggccggcaag aggccgcctt cccaggcgct gcgaccgcgc tgttcacgca
     4621 agaggtcatc accggtgatg acggcctcgc gaagttcgcg ctgctcgacg gcatgcagct
     4681 ccagcagagc tatcgccttc gcgtgattcc gcccgcgagc tcggacctcg gcgtcgtgtt
     4741 cggcgcggag ctcgatctgc agaagccggc gatggtccag ctcccgaaac gcgtgaagct
     4801 gcgcggccgc gtcctcgatg tcacggggca gccactcgcc gacgtgtctg tcacagcgcg
     4861 ccgctcgctc cgcttcttgt ggagcctcga cagcgacaat caagagtttc tcgacgagat
     4921 tccggcggcc acggccatca cgcaggaggc cggcgacttc gtcgtgtggg tcgaccctgc
     4981 ccttgccgac gcgtgggcgt tctacgacct gtcattcaag acgccgacgg gctcgagcgc
     5041 accgagctgg atgatccccg acttccagat cccgcgcctc gcgacccaga ccatggtcga
     5101 ggtgaacgac gtcgtgatcc ccgacggagc gtatgtgcga ggcaaagtgg tcgatcctgc
     5161 aggcaatccc gtcgagggca gcgcgctgcg cgtgttcctg ctcccgacga ccgaccagct
     5221 ctgttacgag gtcacgcacg cgcccgacaa ctgctccgtc gaggcgaccg tgatgggcga
     5281 cggcgagtcg gatatgcagg ggatcgtccg gctcgacctg ccacgtccgt agctgtccta
     5341 agcaagaagc tggctcctcc cgagtcttgc cttgacctgc ccctcgacgg cgagtagggt
     5401 ctggccaact caggaagtaa tgccgaccgt taggacacag cagagcaacg tacgcgaaac
     5461 ggaagattcc gtgcagcgcg agctctgcct cgattccgct acgccgggca gcaaccagcg
     5521 acgtcgcttg gttcctgcga accctggctc cgcgattcga ccagcggccc gactggatga
     5581 tctcgggact gatcgatttt tcaaggtttc tagtgatgac aaacgatacg acgccgaccg
     5641 agaacgtgtc ggcggacgcc gccgccctag tcgcggcgat gcagaccgaa acgaccgaag
     5701 ccacctctga cacgccggtt gagccgaagc caccgagctt cgacgacctc ggactccatc
     5761 ccgacgtcaa gcaagcgctt gatgacatgg gctacttcgc accgacgccg gtgcagacgg
     5821 ccgtgttcac gccggtcagc gagggcaaga acctcctggt ccagtcgcgt accggtacgg
     5881 gcaagacgac cgcgttcggt ctcccgctcg tgaacaagat ggtgccgacg caccgccagc
     5941 cgcaagcgct gatcctcgcg ccgacgcgcg agctcgccct tcaggtcgcg cgcgagctaa
     6001 cgaacatcgg caagcatcgc ggcatcgtga tcgaggccgt gtacggcggc gctccgatcg
     6061 gcaagcagat caccgcgctc cgcaacggcg tgcacatcgt cgtcggtacg ccgggccgtg
     6121 ttctcgacca catcggtcgc cgcacgctcg actgcaagac gatctcgacg ttcgtgctcg
     6181 acgagtgcga cgagatgctg tcgatgggct tcctcgagga catcgagaag atcacgtcga
     6241 acctgcccga caagcatcag acgcttctgt tctcggcaac gatgcccgac gaggtgacgc
     6301 gttatgcgcg ccgccacatg caggagcgcg agcaaatctc gctctcgcgt gactcggtct
     6361 ccgtctcgga catccaccac gcgtactaca tcgtctcggg catcgcgcgt agccgcgacc
     6421 tcctgaagat cctgttcgcg gagaatcccg agagcgcgat catcttctgc aacatgcgcg
     6481 acgaaacgac gatggtcgcg aagatgctgc agaagcaggg gctcgatgcc gagccgctct
     6541 ccagcgatct gtcgcagaac gatcgcgagc gcgtgatggc ccgcatgaag gcgaagaacc
     6601 ttcgcttcct atgcgccacc gacgtcgccg cgcgcggcat cgacatctcg aacctctcgc
     6661 acgtgatcaa cttctcggtg ccggagagcc ccgaggtcta cgtgcaccgc acgggtcgta
     6721 cgggtcgcgc cggcaagaag ggcactgccc tctcgctcgt cggtccgcgc gagctcggca
     6781 acttccgtta tgtgcgcctc caattcggca tcaagccgga agagcgcctg ctgccgaagg
     6841 acgacatctt cgtcggcaag ctgaaagtcc cgctgccgcc ggtcggcgtt ccgcagccgc
     6901 ccgacccggt ccagattctc atccgtggcg tccctggtac gccgagcgac ctcgagcgcc
     6961 agatcttcga gaagctgctg cagaaatctg aaggcaagcg cgtcctcgcg gcgctcgttg
     7021 ccgacaagct gaacgcgctg tcgacgaaga cgtcgcggcc gcgccgcgcc gagcgccccg
     7081 aaggcgacgc cggtgcaggc gaagctggtg gcggcggcga cgaaggtcgt cgtaccggcc
     7141 gcttcgatcg cccggatcgc ccgcgcttcg gcgccgatgg tgaacgtggc gagcgcccgc
     7201 gcttcggcga tggcgagcgt ggtgaccgcg gtgatcgtgg tgaccgtccc cgtttcgatc
     7261 gcgatcgcgg tgatcgtccc cgcttcggcg gcgatgttgg tggcgagcgc ggtgagcgtc
     7321 ctgatcgtgg cgaccgtccg cgcttcggcg ccgatgg
//
LOCUS       DATLBZ010000034        22789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_771927, whole genome shotgun sequence.
ACCESSION   DATLBZ010000034 DATLBZ010000000
VERSION     DATLBZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22789
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771927"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VMZ53_01680"
     CDS             <1..705
                     /locus_tag="VMZ53_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27178.1"
                     /translation="PEVEFFEDFYIDIYENDTFALFNRNELYSIAAADRARKKILLNR
                     PFAEQSEINVRGRVVKSVGRPADIYSLGAVFYYLISGAYANPKNLFDAFRKFVEYERR
                     DENNSIAAYIEHEYRTIQNLRAPKADGTGAPELAPEDRFFSYKHYLDGNGELIDPAIM
                     MIIAKAMIRNKPDSYCLSYDLRTTGISAMVHDLLSLYIAFGIDPSARIAYSNEGYSAP
                     KKSGAMRRAFDKLFKK"
     gene            897..2894
                     /locus_tag="VMZ53_01685"
     CDS             897..2894
                     /locus_tag="VMZ53_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUS27179.1"
                     /translation="MSRIPHLLSATTLCLLASTASANPKLVRHPDVVPGEYIVVLANT
                     PAATANDRAKAIEATDRLVAVHGGAIQHRFTSALRGFSARLTAEQAEALADDPEVAYV
                     EENGRVYADTVQTMPAWGLDRIDQASLPLDQRYLQLGDGAGVTVYVIDTGLRTSHTEF
                     AGRVPDTLYVTSFANAEDCAGHGTHVAGTIAGKTWGVAKGAKIIPVRVLDCNGSGTEA
                     NVILGIDYVAQHHAPLAVANLSLGGGQSVAEDQAVRNLVASGVTTVISAGNSNADACN
                     VTPARVPEAITVAAIGNNDRRANFSNWGACVDIFAPGVMIASAGIVSDTAVNVLDGTS
                     MAAPHVAGVAAAFLGANPTATPAMVAAAIIDGATRDAISDPMGSPNRLLNARIVDRTP
                     PTLSITSPAAGDHVPANFKVLVEASDQNLVAVALKVDEKVIETRESGPFGFDVSALTP
                     GSHTLTVTSADAAGLTGQQMINVIVDGDDDMGTGPEELGGGCSAGGSAALPFALLLVG
                     ALALRRRRVRNILGLAGFSALFIACVVGDEAPPITTTTAWVNPPCANPIVDENQDGIP
                     DGLDLDCDGTVDIDLTGGTGTGGGGSGGGSGSGGGSGGGGSSSVTQCSATIDDQSISC
                     TRIDNNPARCECKIGGQLVSTCTTTSSNACSFPGPDNCCGF"
     gene            2951..5764
                     /locus_tag="VMZ53_01690"
     CDS             2951..5764
                     /locus_tag="VMZ53_01690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27180.1"
                     /translation="MTDTWQHTHVGRFRVEGVLGRGGMGVVLRAYDPHLQRPVAIKVL
                     TERKTTVRAGTTVNLRERVDQDGLLDEARALAQITDPNVLAVHEIGNELGQTFLVMEL
                     VEGVDLRQWLAAAPRSLGDIYQVFAQAGRGLAAAHRRDIIHRDFKPENVLISADGRVR
                     VCDFGIAAFARASELIHTGTAGTPRYMAPELWRDLGASVQSDVYAFAATLVEAIIGEL
                     PTDPAIVDRDLARRGVHARLRATILRALEPDVAKRPAQLDEVVRAFATRQPGRWRVPV
                     VVAAVVIISFIAMTLLLRHNDPSAGVCSDSAELVSARWNDHEREAVRRGVIATHAQPG
                     DADDIIARLDEYARSWQRLRDETCSAKVAQAQRPARLACLDRRLYDLTAVVRALYRTP
                     SRDLAFSRSASLPDLGSCIEATQVRLPAGTATRVAIEELTAKVATLYDQGLVHVGSQA
                     ELADLEDVRTQALALGDIELAVRIDRVRGQLLANATRLADAERVLDDGAQLAMEHHQD
                     VVAALALIEGASVSRIRHDLGAAESKLRIARSQLERSPDATPYSRMRLHRELSNLAAE
                     RGNMPEALEQLQAASAAVASMQPRDPLWTAQLVIARAQLLDAEGKLAEALLVAREAEQ
                     QLRALGSQGVVELSNALRIIGDLERQRGKLDVAQAVFAERVALLERTMPADNSARVYA
                     EGMVGNVLIDRGQFADARDAFKRTLDRIESTPALVDVHTDFLGNVARAERLLGNFERA
                     RVLEQAAIDEERHAGRPIAVAYHEISYTFLELEAGDFRQAALHARVAEELLASDPADT
                     ANRLDLADSQIAIALGENKPAVADQIATRVLALFEERKIDDARNERFWIAAAQARSLL
                     GRADETRELAQRALARRLARNADPFEISVAEVAVGIATYGESRDPAALASLRKHLVTL
                     ADPRTRIEHAQLARWFAANHLRP"
     gene            5788..7071
                     /locus_tag="VMZ53_01695"
     CDS             5788..7071
                     /locus_tag="VMZ53_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS27181.1"
                     /translation="MPPTGPETHGLREDSIFIRRFEVAVVDGPDRGARAVSSDDELTI
                     GTAAGCHLQVTDPAVSRHHCAVRVTARGLELRDHGSTNGTMLGDHEIVRGYIRSGARL
                     RLGATTVVVTILADEIAQPLASGSTFGDLVGASPAMRRLYPMLERGAQSDVTVLIEGE
                     TGTGKELVAEAIHLASARRGKPFVVVDCGALPRQLTESELFGHVRGAFTGAEADRVGA
                     FEQAQGGTIFLDEIGELALDLQPLLLRALENHTIRRVGTNDQREVDVRVVAASHRDLR
                     ALVNEKRFRADLYYRLNVLRVVVPPLRDREGDIRLLATHFWQMFRPDKAAPEDLLLEL
                     EAQTWPGNVRELRNMIERAALIGHTRTALDGEDLPYGEAKERMIADWERRWLTRLLQT
                     HDHNLSRAARAAQMGRSYLRQLVRRYDLSRGDDGE"
     gene            complement(7085..7495)
                     /locus_tag="VMZ53_01700"
     CDS             complement(7085..7495)
                     /locus_tag="VMZ53_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27182.1"
                     /translation="MKQTKFILIAAGVIGVLACFLPYIKEGSLSMSYWDFHKLPKGMA
                     QGLLNGPQQVYVAMVGFLITAVMGVLGMKQLLRWQAIVAIVGCLVALACEGVRKGLSG
                     DHGVSTAIGGKLLFVAAVLGIVGGIIGLAKPDKG"
     gene            complement(7557..7949)
                     /locus_tag="VMZ53_01705"
     CDS             complement(7557..7949)
                     /locus_tag="VMZ53_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27183.1"
                     /translation="MKNLKLGVLVCGVVGLVAVFLPQAELAGRSISLWDAHSQPTSHG
                     GGLHVYMIIAGYVAGIAMGGLALARPPMQRWQSFIALVGFVFVLAKIRHVLPFDIFKQ
                     ALGSKLIGAAAYAGAIISALSLAKRERS"
     gene            8093..8248
                     /locus_tag="VMZ53_01710"
     CDS             8093..8248
                     /locus_tag="VMZ53_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27184.1"
                     /translation="MTSDEAEEFLRGLPLVPAMAGSIPEMKALRDRCLAQDIPALVGC
                     PPGAGKG"
     gene            complement(8451..9308)
                     /locus_tag="VMZ53_01715"
     CDS             complement(8451..9308)
                     /locus_tag="VMZ53_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27185.1"
                     /translation="MKPVVIVLFVGALFLLVGSFTKSWITMSEGDKFSVHAGLRGIEM
                     CGRGECESRSYLDHIDKAKGKELGMALGGAIAAGWGLLAAIMCAIGALMIQTGKGAKG
                     VFAVLGIVFSALAFIGMVIYLGAADKPSEASYGYSMFLFILGLGGAVTGSIMSFSHKL
                     PVAGAMARAGAPGYQQPMGQPAGYGQTPMGYGPGQSGYGQQPPQGYNQQQMGSQPPQA
                     QHGYGQQPQMGGQPQGQQGYGQQPQQQYGGAPQGQPQGAPACNSCGRQTTYVAQYQRY
                     FCQNCNRYL"
     gene            9378..10994
                     /locus_tag="VMZ53_01720"
     CDS             9378..10994
                     /locus_tag="VMZ53_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HUS27186.1"
                     /translation="MSSPPELPAEIAKRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLA
                     GAVKARGDRRRATSDWMKVERERGISVTTSVMTFDYANCTFNLLDTPGHQDFSEDTYR
                     TLTAVDSAVMVIDAAKGIESQTRKLFEVCRMRNVPIATFINKMDREGRDPFELLDEIA
                     NDLQLEVTPASWPIGSGRTFLGCYDLFADKLILMERSKGEYMQEGIEISGLDDPKLDQ
                     LLPESAVAKLREDVAMVRELCPKFDHEMYLEGHQTPVFFGSAVNNFGVQELLQGVAKL
                     APPPRAQPARERAIAPTEPKVSGFVFKIQANMDPKHRDRIAFVRLASGHFTRGMRLTV
                     PRTGKVQSVHNAMVFQANQRELAEDAWAGDIVGIPNHGTLRIGDSITEGEEIHFTGIP
                     SFAPEFLRRVRANDPMKAKHLGRALEQIAEEGAAQVFKMFLGSDYIVGVVGALQFEVL
                     ADRIASEFDLPCHFESTSFEVVRWVDADDPKMIKRFADANRDNIAEDHSGATVFLARN
                     DWQMNRAKQDYPDLRFLQTREQAPLTAASA"
     gene            complement(10999..11664)
                     /locus_tag="VMZ53_01725"
     CDS             complement(10999..11664)
                     /locus_tag="VMZ53_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27187.1"
                     /translation="MPLAADVTIEVNLAYHAKASPLSLAPDLWWNDAHWTIGLTHSMA
                     SVDRFQPGGSLCVRTDELYCDSTYRGSGVDARYAFSLAGDTTVSPRARLLLRDIEPVK
                     PAITAGALVEHTHGRFSFAADPYVQLGLANRDQGNRAALFVPLHVALAVTSRVSPTLD
                     TGWNAELAVLGDGWHVPLALGAEVRIDAHFTVGADVGFAAMLGPQNTPKQRVLFVGVS
                     WRE"
     gene            11665..12399
                     /locus_tag="VMZ53_01730"
     CDS             11665..12399
                     /locus_tag="VMZ53_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUS27188.1"
                     /translation="MATVLLALAACHYDADEADGAFYTWNERQVHCAIDIDTFARNDI
                     ASVEAGLDRARDRGEVLELYMHDPGRTVSWDALEDVLAAAQDRGLAFYTYADLARGTA
                     TPGGGVLLSMDDASLASWIEGVPLYAQYGARMTFFVSYYPQLSMGERAALHDLAAAGH
                     AIEAHSVKHLRAPLYVEQKGLDAYLADEALPSIALLEADGFDVTTYAYPYGSRTGELD
                     RALLRYVDLVRSVAFTWSGPADPCPE"
     gene            12513..13121
                     /locus_tag="VMZ53_01735"
     CDS             12513..13121
                     /locus_tag="VMZ53_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS27189.1"
                     /translation="MGPAVSAFAARGADLALAQRCCDGDKAAQRELFQRLKLGIHHTL
                     FRILGSNRDLEDLIQDAFLEVFRALPSFRGDSTLSRWSQTIATRTAYLAIERRKPTAV
                     DLDLVEEEIVDKDPDQHRIALARHAAKRLYAALDKIDAKQRVAFALAVIDGLSLAEVA
                     ELTESSLVATKTRVWRCRRDLMRRAKKDPVLASYMADLGGVQ"
     gene            13184..13714
                     /locus_tag="VMZ53_01740"
     CDS             13184..13714
                     /locus_tag="VMZ53_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HUS27190.1"
                     /translation="MERNVLSRMEGTITSAVGAREIRGQTATRSNWVWLAVPAAAAAA
                     FALAFFSMNGPATTTQAASNEEPSRIVAGDAPSSVTFGDTHLTLDAHTALVMDQDAAK
                     PKAILENGAAQFTIPARGERQLTVLAGDALVRTGNADFRVSRDGEVVKVSVAAGSVHI
                     TFRGHDVTVGANQNWP"
     gene            13729..14640
                     /locus_tag="VMZ53_01745"
     CDS             13729..14640
                     /locus_tag="VMZ53_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27191.1"
                     /translation="MKTSYWLLSCFAAVALYACGHHSGDGSDANGSGDGDGGNGMIDA
                     GFGADAPFSGTCTPGSSQCSNCNDDDSDGKIDGFDPECTGPLDNDESSFKTGIPGDNI
                     DATSQDCFFDGNSGSGNDGCNQHTCCLLQSGTQPGTTQQDRNECHQEAPSANQNKYTI
                     ANCYAPWGTAPVPTKCSMVCGKLAPPGCDCFGCCTICNPALADTAHPGGCFDIDINPA
                     VSPNCDSSNVNDPAACKQCGKNPDCGNSQCGGATCILCPGQDPSSLPPECNNTTTCPT
                     GIDPCGTDNSCPTGTYCSIGCCIGTLQ"
     gene            complement(14710..16383)
                     /locus_tag="VMZ53_01750"
     CDS             complement(14710..16383)
                     /locus_tag="VMZ53_01750"
                     /inference="COORDINATES: protein motif:HMM:NF027993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HUS27192.1"
                     /translation="MKSLLAGLGLTLVASLNIVGCADSGEETECLPGDIDCAQDSGDG
                     KADGFNGPNDPSRFASQLQYKLSALPKKGKLETPVWKDKYPEAVGKAAVAWADTYWPT
                     SDGSHNARWLGATVKSPVEKYDAAFNNAPGCDTQPPMYGTGAKAKWDTYNNCAGPAAK
                     WQSSVFQGGGKMHDGIDNDGDGKIDDYGSDGIDGIQGWWGTCHAWTPAALMSPEPQHA
                     VTMNGVTFEVGDIKALTQNIFDRTSAVMLGGRCNNKEINHDVNGSANDECADVNPGSL
                     HVILTNFLGLRQAPLIEDRTANYEVWNQPVVAYEVTKQAKITNTAANTCVGSSGSTWK
                     YNTAAKELYEVRVTVTYITETSAGRMPVGFMNSTSQDDYHYILELNAEGKILGGRYCT
                     DSSNSHVDFLWSPTGSNSSSNPSVDVAKVKQLIKASVAPVGTGGGSTTTKEFSASPGS
                     AIPDNAPAGVSVDVPVTGVTGAKSLAVSVDISHTYRGDLTLKLSKDGRAIKTLVADQG
                     GGEDNIVETYNLTAAEIGTDVNGRWTLQVIDDAAQDTGKVNKVTLSFGL"
     gene            complement(16540..18234)
                     /locus_tag="VMZ53_01755"
     CDS             complement(16540..18234)
                     /locus_tag="VMZ53_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27193.1"
                     /translation="MRVQRISLALVLAFVGCGPSGGDHDDKDAVLTVDPPTAELLILD
                     GTPATADFTAVLTYPDGETKDVTDQAHFAVDNGYGMMSGHTGTFATAGKTQVFASLSD
                     KTAASQVIIRLKDIRIDPSLPPDAANWFTSPEDPSRAPTVVYPPANVVMPRNLGDFEV
                     HWTDTANNVFEVSLKTEFTDVRAIVPGGNGAIASGSWLAFLANEWLAAVGYETSVQFQ
                     VRGVDKNNPTVVGAGAPQLVKLSNEAMLGGVYYWASTAQNGTYGIFRHDMGKPGQAAE
                     QFMTTAQTGGRCVACHVLSRDGTRMSITYDGGNGAATLMDVGTKAVQASTRGWNFGAF
                     TPDGAQFLAVQNGSLNVLDSSNQAVLATMTASGTITHPDLSADGKRLVYVQRQGGGDW
                     SFTGGKVFTRTYDQASHTFGPEQMLVSDSNNNYYPSFSPDGKWVLFNRATGGDAYNNG
                     NATLWVIPSDASAAAVQLQSANAGLGLTNSWGRWAPFQQTVGTEPIYWVTVSSTRNFG
                     VRLVGANKPQIWMTPFYPNAASQQQDPSAAAFRLPFQNIDSNNHIAQWTEQIVAPQ"
     gene            18339..19202
                     /locus_tag="VMZ53_01760"
     CDS             18339..19202
                     /locus_tag="VMZ53_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS27194.1"
                     /translation="MRTLTSVLGNGQRLDGGAMFGNAPKAMWEKWIPPDDKNRIPLAC
                     RCLVVRDGTRTILLEAGIGAFFEPSLRERYGVVEDRHVLLESLAALGIRPSDVDVIVL
                     SHLHFDHAGGVLSTWQQGAQPSLVFPNAKYVVGAEAWQRAIQPHARDRASFIPGLTDM
                     MAATGRLEIAAGDRSDVLGDGYRFHRSSGHTPGLLLTEVEMADGPVVFAADLIPGKAW
                     VHLPITMGYDRYPEMLIDEKATLLGNLLERHGRLFFTHDPVVAMGTVAKDERGRFHVV
                     DERETIRDLAA"
     gene            complement(19204..19563)
                     /locus_tag="VMZ53_01765"
     CDS             complement(19204..19563)
                     /locus_tag="VMZ53_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27195.1"
                     /translation="MDAPPAQQGPLEDDLPRLAERAVKMYQDWQKAFAEAGTDCAAAT
                     TKMNALADANADLIAANQRLTKSGHEKIKAARAELEKHDAEIDPAAQAIMQGPTMAAC
                     HDDRAFNTALDRLAGEG"
     gene            complement(19658..19861)
                     /locus_tag="VMZ53_01770"
     CDS             complement(19658..19861)
                     /locus_tag="VMZ53_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27196.1"
                     /translation="MSEIDETQFEGTLVLEQLAAIEKVDEFFEAIDADDERRAVSLMR
                     RANVDAATIEVVLEKMRAADGTH"
     gene            complement(19858..20751)
                     /locus_tag="VMZ53_01775"
     CDS             complement(19858..20751)
                     /locus_tag="VMZ53_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HUS27197.1"
                     /translation="MPKPILFKPVENPYLVPFDGSFKLNKQATEPENKPAKKENVAAL
                     QEYVERLAKLQGKLYAQDRYSVILIFQALDAAGKDGTIKAVMSGINPQGCQVYAFKQP
                     SSLELDHDFLWRVQCALPERGRIGIFNRSHYEEVLVVRVHPEYLEGQRLPYMPKDLEE
                     LWEQRFDSINEAERHWARNGAVILKFFLNVSQKEQHERFLARITDPEHNWKFSASDMK
                     ESEHWDDYQKAYNAALNATSKPWAPWYCIPADSKSYMRRIVAETIVDTLAQLEIDYPT
                     VSPEQKAEMEKVRRKLEAEST"
     gene            20815..21804
                     /locus_tag="VMZ53_01780"
     CDS             20815..21804
                     /locus_tag="VMZ53_01780"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HUS27198.1"
                     /translation="MATTTTTDGSKTDVVPAFSEEDKLGPLGGGKRSLLDDEAELRRV
                     LVVEFQAAADAARDAAGSVDENVTTAVHDFRKALRRARAVLSLTAAALPKSERRAINR
                     ALREARRALGTARDHAVAPDTVDVLPLGDGERDSASSIIRAAADSMPPVAEIKQLLAE
                     GAARAAAQVEALEAALPQTVEWSTVEKGIRSTYRDARTARKSAKRNRRSFHAWRRRSK
                     ELGYQLELLAGYAGTRATELSREIESVTDTQGPAVDLVMLRDFVRTHAAGVESESVHR
                     LVSSIEAQIDDLVADSRRAGREAFRKKPRRFARRLTKAVRRDLAPVVVPDDVD"
     gene            21823..>22789
                     /locus_tag="VMZ53_01785"
     CDS             21823..>22789
                     /locus_tag="VMZ53_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HUS27199.1"
                     /translation="MSKVLIIGAGGVGGVVAHKAAMEKQHFSEVMLASRTLSKVEKIA
                     AEVEQMHGRKIQTRQVDADNVPELTKLLKEYKPAVVINVALPYQDLHIMDACLEAGVD
                     YLDTANYEPPDVAKFEYSWQWAYQDRFKKAGRMALLGSGFDPGVTNVFCAYVQKHLLD
                     EVHYVDIVDCNAGSHGKAFATNFNPEINIREITARGRYWENGEWKETDPLSVSKIFDY
                     PGVGEKKSFLLYHEELESLAKNLKGLKRIRFWMTFGEAYLKHLEVLQNVGMTRIDPVL
                     YNGVEIVPLQFLKAVLPEPSSLGPTTKGKTNIGDIATGEALDGSGE"
BASE COUNT         3909 a         7492 c         7483 g         3905 t
ORIGIN      
        1 cccgaagtcg agttcttcga ggacttctac atcgacatct acgagaacga cacgttcgcg
       61 ctcttcaacc gcaacgagct ctactcgatc gcggcggcgg atcgcgcgcg gaagaaaata
      121 ctgctcaatc gcccgttcgc agagcagagc gagatcaacg tgcgtggccg cgtcgtgaag
      181 agcgtcggcc gccccgccga catctactcg ctcggcgcgg tgttctacta cttgatctcc
      241 ggcgcgtacg cgaacccgaa gaacctgttc gacgcgttcc gcaagttcgt cgagtacgaa
      301 cgtcgcgacg agaacaactc gatcgccgcg tacatcgagc acgagtaccg gacgattcaa
      361 aacctccgtg cgccgaaggc cgatggcacc ggtgcacccg agctcgcgcc cgaggaccgg
      421 ttcttcagct acaagcatta cctcgacggt aacggcgagc tgatcgatcc ggcgatcatg
      481 atgatcattg ccaaggcgat gatccgcaac aagccggaca gctactgcct ctcgtacgat
      541 ctgcgtacga ccggcatctc ggcgatggtg cacgacctgt tgtcgctcta catcgcgttc
      601 ggcatcgacc cgtcggcacg catcgcgtac tcgaacgagg gctacagcgc gccgaagaag
      661 tccggcgcca tgcgtcgcgc gttcgacaag ctgttcaaga agtagcagct ggaccgagct
      721 gtttcgcgcc ggggcctggt tccccgcggt ccagctcctc caccgctcgc tcccacgcgg
      781 gctaagtagg cgcgccgccg cgcgtttgct ggctccaatc gcagcggggg catgtggggt
      841 ctgccggagc gcgcgttgct gactcacgag tcgccatcca acaaaggagt cgacccgtgt
      901 cgcgaatccc tcaccttctg tccgcaacga ccctctgcct gctcgcgagc accgcgagcg
      961 ccaaccccaa gcttgtgcgc cacccagacg tggtgcccgg tgaatacatc gtcgtgctgg
     1021 ccaacacccc ggcggctacc gcgaacgatc gtgcgaaggc gatcgaggcc accgatcgtc
     1081 tcgtcgcggt gcacggcggt gcgatccagc accggttcac gtcagcgctg cgcggcttct
     1141 cggcgcgctt gaccgctgag caggccgagg cgctcgccga cgatccggag gtcgcgtacg
     1201 tcgaggaaaa cggccgcgtc tacgcggaca ccgtgcagac gatgccggcg tggggcctcg
     1261 atcgcatcga tcaagcctcg ctgccgctcg accagcgata tctacagctc ggcgacggcg
     1321 ccggcgtcac ggtttacgtg atcgataccg gcctgcgtac gtcgcacacc gaattcgcgg
     1381 gtcgcgttcc agacacactg tacgtgacgt cctttgccaa tgccgaggac tgcgcgggtc
     1441 acggcacgca cgtcgccggc accattgccg gcaagacgtg gggcgtcgcg aagggcgcga
     1501 agatcattcc ggtgcgcgtg ctcgactgca acggcagtgg caccgaggcc aacgtcatcc
     1561 tcggcatcga ctacgtcgcg caacaccacg cgccgctcgc ggtcgcgaac ctcagcctcg
     1621 gcggaggcca gagcgtcgcc gaggatcagg cggtgagaaa cctcgtcgca tctggtgtca
     1681 cgaccgtgat ctcggcgggc aactcgaatg cggacgcgtg caatgtcacg ccggcgcgcg
     1741 tgcccgaggc gatcaccgtc gcggcgatcg gcaacaacga ccggcgcgca aacttctcga
     1801 actggggtgc gtgcgtcgac atcttcgcgc cgggcgtcat gatcgcctcc gccggaatcg
     1861 tttccgacac cgccgtgaac gtgctcgacg gaacctcgat ggctgcgccg cacgtcgctg
     1921 gtgttgcggc tgcgttcctc ggtgcaaatc cgaccgccac ccctgcgatg gtggcagcgg
     1981 ccatcatcga cggtgcgacg cgcgatgcga tcagcgatcc gatgggctcc ccgaatcgac
     2041 tcctcaatgc gaggatcgtc gaccgcacgc cgccgacact gtcgatcaca tcgcctgccg
     2101 ccggcgatca cgtaccggct aacttcaaag tgctcgtcga ggctagcgat cagaacctcg
     2161 tcgccgtggc tctaaaagtc gacgagaagg tgatcgagac acgcgagagt ggcccgttcg
     2221 gatttgacgt ctcggcgctc acgccgggct cgcacacgct gaccgtgacg agcgcggatg
     2281 cagcgggcct caccgggcaa cagatgatca acgtcatcgt cgacggtgat gacgacatgg
     2341 ggaccggtcc tgaagagctc ggcggaggct gctcggcggg aggcagtgcg gcactgccgt
     2401 tcgcgctgct gctcgtcggt gcgctcgcgc tgcgccggcg ccgcgttcgc aacatcctcg
     2461 gtctggccgg cttcagcgcg ctgttcatcg cgtgcgtcgt cggagacgaa gcgccgccga
     2521 tcacgacgac gacggcgtgg gtgaacccgc cctgcgcgaa tcccatcgtc gacgagaacc
     2581 aggacggcat ccccgacggc ctggacctcg attgcgacgg cacggtcgac atcgatctga
     2641 ccggtggcac cggcaccggc ggcggtggca gcggcggcgg cagtggcagc ggaggaggca
     2701 gcggcggtgg gggcagcagc tcggtcacgc agtgcagcgc gacgatcgat gaccaatcga
     2761 tctcgtgcac gcgcatcgac aacaaccctg cgcggtgcga gtgcaaaatc ggcggccagc
     2821 tcgtcagcac ctgcacgacg acgtcgtcga atgcgtgctc gttccccggt cccgacaact
     2881 gctgcggttt ctaggcagga gaacgacctt gttgccccgc cactgcttcg ttaccctgga
     2941 gctgagccgc gtgaccgaca cctggcaaca cacgcacgtc ggacggtttc gcgtcgaggg
     3001 tgtgctcgga cgcggcggca tgggcgtcgt gcttcgcgcc tacgaccctc acttgcagcg
     3061 gccggtcgcg atcaaggtgc tcacggagcg gaaaacaacg gtcagggctg gcaccacggt
     3121 gaatctgcgc gagcgtgtcg atcaggacgg cttgctcgac gaggcgcgcg cgctcgcgca
     3181 gatcaccgac ccgaatgtgc tcgccgtgca cgagatcggc aacgagctcg gacagacctt
     3241 tctcgtgatg gagctcgtcg agggcgtcga cctgcggcaa tggctcgccg ccgcgccgcg
     3301 tagcctcggc gacatttatc aggtgttcgc gcaagcggga cgcgggctcg cagcggcgca
     3361 ccggcgcgac atcattcacc gcgacttcaa gcccgagaat gtgctgatca gcgccgatgg
     3421 tcgcgtgcgt gtctgcgact tcggcatcgc ggccttcgcg cgcgccagcg agctgatcca
     3481 caccggcact gcggggacac cgcgttacat ggctcccgag ctatggcggg acctcggagc
     3541 gagcgtgcag tccgatgtct acgcgttcgc ggcgacgctc gtcgaggcga tcatcggtga
     3601 gctaccgacg gaccccgcca tcgtcgaccg cgacctcgcg cggcgcggcg tccacgcgcg
     3661 actacgggca acaatcttgc gagcgctcga acccgacgtt gcgaagcgac cggcgcaact
     3721 cgacgaggtc gtgcgtgcgt tcgcgacccg gcagcctggg cgctggcgcg ttccggtcgt
     3781 cgtcgcggcg gtcgtcatca tcagcttcat cgcgatgacg ctgctcttgc gacacaacga
     3841 tccttctgct ggcgtgtgca gcgactcggc agagctcgtc agtgcgcggt ggaacgacca
     3901 cgagcgtgaa gccgtacgcc gcggcgtcat cgcgacgcac gcacaacccg gtgacgccga
     3961 cgacatcatc gcgcggctcg acgagtacgc acggtcgtgg caacgcctac gggatgagac
     4021 gtgcagcgcg aaggtcgcgc aagcacagcg ccctgcccgt ctcgcgtgtc tcgatcgccg
     4081 cctctacgat ctgaccgccg tggtgcgcgc gctgtaccgg acgccgagcc gcgatctcgc
     4141 attcagccgc agcgcctcgt tgccggacct cggctcgtgc atcgaggcta cgcaggtgcg
     4201 tctgcccgca ggcacggcaa cgcgcgtcgc gatcgaagag ctgaccgcga aggtcgccac
     4261 gctctacgat caaggcctcg tgcatgtcgg gtcacaagcg gagctcgcgg atctcgaaga
     4321 tgtgcgtacg caagcgctgg cgctcggcga catcgagctc gcggtgcgca tcgaccgcgt
     4381 gcgtgggcag ttgctcgcga acgcgacccg tctggcagac gcggagcgcg ttctcgacga
     4441 tggggcacag ctcgcgatgg agcatcatca agatgtcgtt gccgcactcg cgctcatcga
     4501 aggcgcatcg gtgtcccgca tccgccacga tctcggtgca gcagaatcca agctacgtat
     4561 cgcgaggagc cagctggagc gctcgcccga cgcgacgccg tatagccgca tgcgactcca
     4621 ccgcgagctg tcgaaccttg cggccgagcg cggcaacatg ccggaggcgc tcgagcagct
     4681 tcaagccgca agtgccgcgg ttgcttcgat gcagccgcgc gatccgctgt ggaccgcgca
     4741 actcgtgatc gcgcgcgcac agctgctcga tgcagagggc aagctggctg aagcgcttct
     4801 ggtcgcccgc gaggcggagc aacagctgcg cgcactcggc agtcaaggtg tcgtcgagct
     4861 ctcgaacgcg ctgcgcatca tcggtgacct cgagcgccag cgaggcaagc tcgatgtcgc
     4921 gcaggcggta ttcgccgagc gcgtcgcgtt gctcgagcgc acgatgcccg cggataactc
     4981 ggcacgcgtc tacgccgagg gcatggtcgg caatgtcctc atcgatcgcg gccagttcgc
     5041 agacgcgcga gacgcgttca agcgcacgct cgatcgcatc gagagcacgc ccgcgctcgt
     5101 cgacgttcac accgatttcc tcggcaatgt cgcgcgtgcc gagcgcctgc tgggcaactt
     5161 cgagcgtgcg cgtgtgctcg agcaggctgc gatcgacgag gaacgtcatg cgggtcgccc
     5221 gatcgcggtc gcgtaccacg agatctcgta cacgttcctg gagctcgagg cgggtgactt
     5281 ccggcaagct gcgctccacg cgcgcgtcgc agaggagctg ctcgcgagcg acccggccga
     5341 caccgccaac cgcctcgatc ttgcagactc gcagatcgcg atcgcgctcg gcgagaacaa
     5401 gccggcagtc gccgatcaga tcgcgacccg cgtgctggcg ctgttcgagg aacgcaagat
     5461 tgatgacgcc cgcaacgagc ggttctggat cgctgccgcg caggcgcgca gtcttctcgg
     5521 ccgcgcggat gaaacgcgcg agctcgcgca acgagcgctt gcccgccggc tcgcgcgcaa
     5581 cgcagaccct ttcgagattt cggtcgccga ggtcgcggtt gggatcgcaa cgtacggcga
     5641 atcgcgcgac cccgcggcgc tggcgagctt gcgcaagcac ctcgtcaccc ttgcggatcc
     5701 tcggacgcga atcgagcacg cacagctggc gcggtggttt gccgcaaacc acctgcggcc
     5761 ttgatggctc tggactacca ttcacggatg cctcctaccg ggcccgagac gcacggcctg
     5821 cgcgaagact cgatcttcat ccgccggttc gaggtggccg tcgtcgacgg tccggaccgc
     5881 ggcgcgcgcg cggtgtcatc cgacgacgag ctgacgatcg gcaccgcggc aggctgtcat
     5941 ctccaggtca ccgatcccgc ggtgtcgcgc catcactgcg cggtccgggt gaccgcacgt
     6001 ggcctcgagt tgcgcgatca cggcagcacg aacggcacga tgttgggcga tcacgaaatc
     6061 gtacgcggct acattcgcag cggcgcacga cttcgcctcg gcgcgacgac cgtcgtcgtc
     6121 acgatcctcg ccgacgagat cgcccagccc ctcgcaagtg ggagcacttt cggcgacctc
     6181 gtcggtgcga gccccgccat gcggcgcctc tatccgatgc tcgagcgcgg tgcgcagagc
     6241 gacgtgacgg tgctgatcga aggcgagact ggaaccggca aagagctcgt cgccgaggcc
     6301 attcacctgg cgagcgcgcg ccgaggcaaa ccgttcgtcg tcgtagattg tggcgcgctg
     6361 ccgaggcagc tcacggaatc ggagctattt ggccacgtgc gcggtgcgtt caccggtgcg
     6421 gaggccgacc gcgtcggcgc gttcgagcag gcgcagggcg gcacgatctt cctcgacgag
     6481 atcggcgagc tcgcgctcga tctccagccg ctgctgcttc gcgctctcga gaaccacacg
     6541 atccgccgcg tcggcacgaa cgatcagcga gaggtcgacg tccgcgtcgt ggccgcaagc
     6601 catcgcgacc tgcgtgcgct cgtcaacgag aagcgctttc gcgccgacct ctactaccgg
     6661 ctcaacgtgc tccgcgtcgt cgtgccgccg ctccgtgatc gcgaaggaga cattcgcctg
     6721 cttgcaactc acttctggca gatgttccgt ccggacaagg ccgcacccga agatctgctc
     6781 ctcgagctcg aagcacagac gtggcccggc aacgttcgcg agctccgcaa catgatcgag
     6841 cgcgcggcgc tcatcggcca cactcgcacg gccctcgacg gtgaagacct gccgtacggc
     6901 gaagcgaagg aacgtatgat cgcggactgg gagcggcgtt ggctgacgcg cctcctccaa
     6961 acccacgatc acaacctgtc gcgcgccgct cgtgcagcgc agatggggcg cagctaccta
     7021 cgccagctcg tccgccgcta cgacttatcg cgaggcgacg acggcgagta gatcgccttc
     7081 gggcttagcc cttgtcgggc ttcgcgaggc cgatgatgcc gccgacgatg ccgagcaccg
     7141 ccgcgacgaa gagcagcttt ccgccgatcg cggtgctcac gccatgatcg ccgctgaggc
     7201 ccttgcgaac gccttcgcag gcgagtgcga ccaggcagcc gacgattgcg acgatcgcct
     7261 gccagcgcag tagttgcttc atgccgagca cgcccatcac ggccgtgatc aagaagccga
     7321 ccatcgcgac gtagacctgc tgcggtccat tcaagaggcc ctgggccatg cccttcggca
     7381 gcttgtggaa gtcccagtag ctcatcgaga gcgagccttc cttgatgtac ggaaggaagc
     7441 acgcgagcac tccgatgaca ccggcagcga tcagaatgaa tttggtctgc ttcatggcct
     7501 cacacgttat cagttgagtt gttgatggtc ctgattctga gtcgtagtgt aattttttag
     7561 gatctctctc gcttcgcgag cgagagcgcg gagatgatcg cgccggcgta cgccgcagcg
     7621 ccgatgagct tgctgccgag tgcttgcttg aagatgtcga acggaagcac gtggcggatt
     7681 tttgcgagga cgaacacgaa cccgacgagc gcgatgaacg actgccagcg ctgcatcggt
     7741 ggacgcgcga gcgccaagcc gcccatcgcg atacccgcca cgtagcccgc gatgatcatg
     7801 tagacgtgca gcccgccgcc gtgcgaggtc ggttgcgagt gtgcgtccca tagactgatc
     7861 gaacgaccag cgagctctgc ttgcggcaag aacaccgcaa cgagaccgac aacaccgcag
     7921 acgagcacac cgagcttgag attcttcacc gtccgtcttc ccctcagctg gggcgatgct
     7981 ccccttagag caaacgcggc gccagtatcc acgaaaacga gtccgcgcgc ttagagatca
     8041 tttctgggtc cctccggcgg caatgggcgc tatcttgggt tcgagcggac ccatgacgag
     8101 cgacgaagcc gaagagtttt tgcgcggcct gcccctggtt ccggcgatgg ccggatcgat
     8161 ccccgagatg aaagcgctgc gcgatcggtg cctcgcgcag gacatccccg cgctcgtcgg
     8221 ctgcccgccg ggcgccggca aaggttgagg tacgaagacg cacctcctgg tcgaggagga
     8281 cgatctcgag aagctcgcga acatcctgcg cggtgattgg cgcgaggcgc tcgcgcgcga
     8341 aggtgcggcc gaggtcgatc tcagcggcga cgcgtgtccc gcgtgtggct gcaaattgcc
     8401 gctcgtcgag ggcgcgtgcg ccgactgcgg ccttcagctc gagtagtccg ctagaggtag
     8461 cggttgcagt tctggcagaa gtagcgctgg tactgcgcga cgtacgtcgt ctggcgaccg
     8521 cacgagttgc acgcgggtgc gccctgcggt tggccctgcg gtgcgccacc atattgctgc
     8581 tgcggttgct ggccgtagcc ctgttggccc tgcggctggc cgcccatctg cggttgctgg
     8641 ccgtagccgt gctgcgcttg cggcggttgg ctgcccatct gttgctggtt gtagccctgc
     8701 gggggttgct ggccgtagcc gctctggccg gggccgtagc ccatcggcgt ttggccgtag
     8761 ccagcgggct ggcccatcgg ctgctggtag cccggagcgc ccgcacgagc catcgcgccg
     8821 gcgaccggca gcttgtggga gaacgacatg atcgagcccg tgacggcacc gccgaggccc
     8881 aggatgaaca ggaacatcga atagccgtag gaggcttcac tcggtttgtc ggcggctcct
     8941 aggtagatga ccatgccgat gaacgcgagc gcggagaaca cgatcccgag caccgcgaag
     9001 acgcccttcg cgcccttgcc cgtctggatc atgagcgcgc cgatggcgca catgatggcc
     9061 gcgaggaggc cccaacctgc cgcgatcgcg ccaccgagcg ccatcccgag ctctttgccc
     9121 ttcgccttgt cgatgtgatc gaggtagctg cgcgattcgc actcgccgcg accgcacatc
     9181 tcgatgccgc gcaggccagc gtggacgctg aacttgtcgc cttcgctcat cgtgatccac
     9241 gacttggtga acgacccgac caagaggaac agcgccccga cgaagaggac gatcaccacc
     9301 ggcttcatgc gggcatcata cgcgggtctt gccgtcgctg ttgccgcggc gccatcgaaa
     9361 ccgtagattc cggcgccatg tcgtccccgc ccgagctacc cgccgaaatc gcgaagcgcc
     9421 gtaccttcgc gatcatcgcg caccccgacg ccggtaagac gacgttgacg gagaaactct
     9481 tgctgttcgg cggcgcgatt cagctcgcgg gtgcggtcaa agcgcgaggc gatcgccgcc
     9541 gcgcgacgtc ggactggatg aaggtcgagc gcgagcgcgg catctccgta acgacgtccg
     9601 tgatgacgtt cgactacgcc aactgcacgt tcaacctgct cgacacgccg ggccaccaag
     9661 acttcagcga ggacacatat cgcacgctca ctgccgtcga ctcggccgtc atggtgatcg
     9721 acgcagcgaa gggcatcgag agtcagacgc gcaagctgtt cgaagtctgc cgcatgcgca
     9781 acgtgccgat cgcgacgttc atcaacaaga tggatcgcga aggccgtgac ccgttcgagc
     9841 tgctcgacga gatcgccaat gacctgcagc tcgaagtcac gcccgcgagc tggccgatcg
     9901 gcagtggtcg gacgttcctc ggctgctacg acctgttcgc cgacaagctg atcctcatgg
     9961 agcgcagcaa gggcgagtac atgcaggagg gcatcgagat ctccggcctc gacgatccga
    10021 agctcgacca gctcctcccc gagtccgcgg tcgcgaagct gcgcgaggac gtcgcgatgg
    10081 tgcgcgagct gtgccccaag ttcgatcacg agatgtatct cgagggccac cagacgcctg
    10141 tcttcttcgg cagcgccgtg aacaacttcg gcgtgcagga gctcctgcag ggtgtcgcga
    10201 agctcgcgcc gccgcctcgc gcgcagcccg cacgcgagcg tgcgatcgca ccgacggagc
    10261 cgaaggtctc gggcttcgtg ttcaagattc aagcgaacat ggatccgaag catcgcgatc
    10321 gcatcgcgtt cgtgcgcctc gcatctggcc acttcacgcg gggcatgcgc ctcaccgtcc
    10381 cgcggacggg caaagtccag agcgtgcaca acgcgatggt gttccaggcc aaccagcgcg
    10441 agctcgccga ggacgcgtgg gccggcgaca tcgtcggcat tccgaaccac ggcacgctgc
    10501 gcatcggcga ctcgatcacc gaaggcgaag agatccattt cacgggcatc ccgagcttcg
    10561 caccggagtt tctgcgtcgc gtgcgcgcga acgatccgat gaaggccaag cacctcggcc
    10621 gcgcgctcga gcagatcgct gaagaaggcg ccgcacaggt gttcaagatg ttcctcggca
    10681 gcgactacat cgtcggtgtc gtcggcgcgc tccagttcga ggtcctcgcc gatcgcatcg
    10741 cgagcgagtt cgacctgccc tgccacttcg agtcgacgtc gttcgaggtc gtgcgctggg
    10801 tcgacgcgga cgatccgaag atgatcaagc gctttgccga cgcgaaccgc gacaacatcg
    10861 ccgaggatca ctcgggcgcg acggtcttcc tcgcacgcaa cgactggcag atgaatcgcg
    10921 cgaagcagga ctatccagac ctgcgcttct tgcagacgcg cgagcaagcg ccgctcaccg
    10981 ccgcttccgc gtagccagtc actcgcgcca cgagacgccg acgaacaaca cgcgctgctt
    11041 gggcgtgttc tgcgggccga gcatcgccgc aaacccgacg tcagcgccga ccgtgaagtg
    11101 cgcatcgatg cgaacctctg cgccgagcgc gagcggcacg tgccagccgt cgccgagcac
    11161 cgcgagctcg gcattccagc cggtgtcgag cgtcggcgag acgcgcgatg tcacggcaag
    11221 cgcgacgtga agaggcacga acagcgcggc gcggttgccc tggtcgcgat tcgcgaggcc
    11281 gagctgcacg tatggatcgg cggcgaacga gaagcggcca tgcgtgtgct cgacgagcgc
    11341 accagcggtg atcgcgggct tcaccggctc gatatcgcgg agcagcagcc gcgcgcgcgg
    11401 cgagacggtc gtatcacccg cgagcgagaa cgcgtagcgt gcgtcgacgc cggagccgcg
    11461 atacgtcgag tcgcagtaca gctcgtcggt gcggacgcaa aggctgccgc ccggctgaaa
    11521 tcgatctacg ctcgccatcg agtgcgtgag gccgatcgtc cagtgcgcgt cgttccacca
    11581 gagatccggt gcgagcgaga gcgggctcgc cttggcgtgg tacgcgaggt tcacctcgat
    11641 cgtcacgtcc gccgccagtg gcacgtggcc acggtactcc tcgcgctcgc tgcatgccac
    11701 tacgacgccg atgaggccga cggcgcgttc tacacgtgga acgagcgcca ggttcactgc
    11761 gcgatcgata tcgacacctt cgcgcgcaac gacatcgcca gcgtcgaggc cgggctcgat
    11821 cgcgcacgcg accgcggcga ggtgctcgag ctctacatgc acgacccggg acgaacggtg
    11881 tcgtgggacg cgctcgagga cgtgctcgcc gccgcgcagg atcgcggtct cgcgttctac
    11941 acgtacgcgg atctcgcgcg cggcactgcg acgcccggcg gtggcgtgct gctctcgatg
    12001 gacgacgcat cgctcgcgag ctggatcgaa ggcgtgccgc tctacgcgca gtacggagcg
    12061 cgcatgacgt tcttcgtctc gtactacccg caactgtcga tgggcgagcg cgcggcgctc
    12121 cacgatctgg cggccgcggg ccacgcgatc gaagcgcaca gcgtgaagca cctgcgcgcg
    12181 ccgctgtacg tcgagcagaa gggcctcgat gcgtatctcg cggatgaggc gctgccatcg
    12241 atcgcgctgc tcgaagcgga cggcttcgac gtcacgacgt acgcgtatcc gtatggctcc
    12301 cgaaccggcg agctggaccg cgcgctcctc cgctatgtcg atctcgtgcg atcggtcgcg
    12361 ttcacgtggt cggggcctgc ggatccatgc ccggagtgag cacagccacc tacacgaatg
    12421 ccgaattgtt ccgtgattgc agcatcatct gcgctgtctt cgcagcctga ctttcttgcg
    12481 actttcgatc gtttgaggcc cactagatca acgtgggacc cgctgtatcc gcattcgcgg
    12541 cacgaggagc cgacctcgcg ttggcgcagc gttgctgcga cggcgacaag gctgcgcagc
    12601 gcgagctgtt ccagcggctg aagctcggca tccatcacac gctgttccgc atcctcggaa
    12661 gcaatcgcga cctcgaggat ctgatccaag acgcgttcct cgaggtgttc cgcgcgctgc
    12721 cgtcgttccg cggtgacagc acgctctcgc ggtggtcgca gacgatcgcg acgcgcacgg
    12781 cgtatctcgc gatcgagcgt cgcaagccga cggccgtcga cctcgatctc gtcgaagaag
    12841 agatcgtcga caaagatccc gatcagcatc gcatcgcgct cgcgcgccac gccgcgaagc
    12901 gtctctacgc cgcgctcgac aagatcgacg caaagcagcg cgtcgcgttt gcgctcgcgg
    12961 tgatcgatgg cttgtcactc gcggaagtgg ccgagctgac cgagagctcg ctcgtcgcga
    13021 ccaagacgcg cgtgtggcgc tgccgccgcg acctcatgcg tcgcgcaaag aaggatccgg
    13081 tcctcgccag ttacatggct gaccttggag gtgtgcaatg acggaacgtg acaggttggg
    13141 gccgccaccg gtggacgcga tgtccgacgt ggcgtgggcg cgcgtggaac gcaacgttct
    13201 ctcgcgcatg gaaggaacga tcacgagcgc cgtgggtgcg cgcgagattc gcggccagac
    13261 cgcgacgcgc agcaactggg tgtggcttgc agtgccggcc gcggcggccg ctgcattcgc
    13321 gctcgcgttc ttctcgatga acggcccggc gacgacgacg caggccgcga gcaacgaaga
    13381 gccatcgcgc atcgtcgcgg gggacgcgcc gtcgtcggtg acattcggcg atacgcacct
    13441 gacgctcgac gcgcacaccg cgctcgtcat ggatcaagac gcggcgaagc cgaaggcgat
    13501 cctcgagaac ggcgcggcgc agttcacgat tccggcacgc ggcgaacgcc agctgacggt
    13561 gcttgccggc gacgcgctcg tgcgcacggg caatgccgac tttcgcgtgt cgcgcgacgg
    13621 cgaggtcgta aaggtgtccg tcgcagcagg cagtgttcac atcaccttcc gaggtcacga
    13681 cgtcactgtc ggggccaacc aaaattggcc ctaggagcaa acaagaacat gaaaaccagt
    13741 tattggcttt tgagctgttt cgcggccgtc gcgctctacg cgtgcggtca ccacagcggt
    13801 gacggcagcg acgctaatgg cagtggcgac ggcgacggcg gcaacgggat gatcgacgca
    13861 ggctttggcg ccgacgcgcc gttcagcggc acctgcacgc cgggctcgtc gcagtgctcc
    13921 aactgcaacg acgacgacag cgacggcaag atcgacggct tcgatccgga atgtacgggc
    13981 ccgctcgata acgacgagag ctcgttcaag accggcatcc cgggcgacaa catcgacgcg
    14041 accagccagg actgcttctt cgacggcaac tccggctcgg gcaacgacgg ctgcaaccag
    14101 cacacgtgct gcctcttgca gtcgggcacg cagcccggca cgacgcagca ggatcgcaac
    14161 gagtgccacc aggaggcacc gtcggcgaac cagaacaagt acacgatcgc gaactgctac
    14221 gcgccgtggg gcacggcacc ggtgccgacg aagtgcagca tggtctgcgg caagctcgcc
    14281 cctccaggct gcgactgctt cggttgctgc acgatctgca acccggcgct cgccgacacg
    14341 gcgcaccccg gtggttgctt cgacatcgac atcaacccgg ccgtctcgcc gaactgcgat
    14401 tcgtcgaacg tgaatgatcc ggcagcgtgc aagcagtgcg ggaagaaccc cgactgcggc
    14461 aactcgcagt gcggcggcgc gacatgcatc ctgtgcccgg gccaggaccc gagctcgctg
    14521 ccgccggagt gcaacaacac gacgacgtgc ccgaccggca tcgacccgtg cggcacggat
    14581 aacagctgcc cgaccggcac gtactgctcg atcggttgct gcatcggcac gcttcagtaa
    14641 gtcgcgcgac cgaaacgcca aacgccccgt gaattccacg gggcgttttt cgttttggag
    14701 cgttacgact tagaggccga acgagagggt gaccttgttc accttgccgg tgtcctgcgc
    14761 tgcgtcgtcg atgacctgca gcgtccagcg accgttcacg tcggtgccga tttcggcggc
    14821 cgtcaggttg tacgtctcga cgatgttgtc ctcgccgccg ccctggtcag ccacgagcgt
    14881 cttgatcgcg cggccgtcct tcgagagctt cagcgtgagg tcgccacggt acgtgtgcga
    14941 gatgtcgacc gagaccgcga ggctcttcgc gccggtgacg ccggtgaccg ggacgtcgac
    15001 gctgacgcct gcgggcgcgt tgtcggggat tgccgagccg ggcgacgccg agaattcttt
    15061 cgtcgtcgtc gagccaccgc cggtgccgac cggagcgacc gaggccttga tgagctgctt
    15121 caccttcgcg acatcgacgc tcgggttcga cgacgagttc gagcccgtgg gcgaccagag
    15181 gaagtcgacg tgcgagttcg aggagtccgt gcagtagcga ccgccgagga tcttgccctc
    15241 tgcgttgagc tcgaggatgt agtggtagtc gtcctgcgac gtgctgttca tgaagccgac
    15301 cggcatgcgg cccgcgctcg tctccgtgat gtacgtgacg gtgacgcgaa cttcgtagag
    15361 ctccttcgcg gccgtgttgt acttccacgt cgagcccgac gagccgacgc acgtgttcgc
    15421 ggcggtgttc gtgatcttcg cctgcttcgt cacttcgtac gcgacgaccg gctggttcca
    15481 cacttcgtag ttcgccgtgc ggtcctcgat gagcggcgct tggcgcaggc cgaggaagtt
    15541 cgtcaggatg acgtgcagcg agcccgggtt gacgtccgcg cactcgtcgt tcgccgaacc
    15601 attgacgtcg tggttgatct ctttgttgtt gcagcgacca ccgagcatga cagcgctcgt
    15661 gcggtcgaag atgttctgcg tcagcgcctt gatgtcaccg acttcgaacg tcacgccgtt
    15721 catcgtgacc gcgtgctgcg gctcgggcga catgagcgcg gccggcgtcc acgcgtggca
    15781 ggtgccccac cagccttgga tgccgtcgat gccgtcgctg ccgtagtcgt cgatcttgcc
    15841 gtcgccatcg ttgtcgatgc catcgtgcat cttgccgccg ccttggaaca cggacgactg
    15901 ccacttggcc gcggggcccg cgcagttgtt gtacgtgtcc cacttggcct tcgcaccggt
    15961 gccgtacatc ggcggctgcg tgtcgcaacc aggcgcgttg ttgaatgctg cgtcgtactt
    16021 ctcgaccggc gacttcacgg tcgcaccgag ccagcgcgcg ttgtgcgagc cgtccgaggt
    16081 cggccagtac gtatcggccc acgcgacagc ggccttgccg actgcctcgg gatacttgtc
    16141 cttccacacc ggcgtctcga gcttgccctt cttggggagc gccgagagct tgtactgcag
    16201 ctgcgaagcg aagcggctcg ggtcgttcgg accattgaag ccgtccgcct tgccatcgcc
    16261 cgagtcctgc gcgcagtcga tgtcgcccgg caagcactcg gtctcctcgc cgctatctgc
    16321 acagccgacg atattgagag aggctacgag ggtgagtccg agtccggcga gtaggctttt
    16381 catggctgca gtctccaaaa cgagaggttg ttgatgcaca gcggcaaagc accccctgtg
    16441 ccagcgcgca cgccaaccct ttcgccctct tagatgtgag gacgatcggc tgcacctctc
    16501 gctgtagccg gcagatcgat cagccggtgc gtcagctcgt cactgcgggg cgacgatctg
    16561 ctcggtccac tgcgcgatgt gattattgct gtcgatgttc tggaacggca ggcggaacgc
    16621 cgcggcgctc ggatcctgct gttgcgacgc agcgtttgga tagaacggcg tcatccagat
    16681 ctgcggcttg ttggcaccga cgagacgcac gccgaagttg cgggtgctcg agaccgtgac
    16741 ccagtaaatg ggctcggtgc cgaccgtttg ctggaacggc gcccagcgtc cccacgagtt
    16801 cgtgaggcca aggcccgcgt tcgcggactg cagttgaacc gcggccgcgc tggcgtcgga
    16861 cggaatcacc cacagcgtcg cgttgccgtt gttgtacgca tcgccgccag tggcgcggtt
    16921 gaagagcacc cactttccgt cgggcgagaa cgatgggtag tagttgttgt tcgagtccga
    16981 cacgagcatc tgctcgggac cgaacgtgtg cgacgcctga tcgtacgtgc gcgtgaagac
    17041 cttgccgccg gtgaacgacc aatcgccgcc accttgacgc tggacgtaga cgagacgctt
    17101 accgtccgcc gaaagatcgg gatgcgtgat cgtgcccgac gccgtcatcg tcgcgagcac
    17161 cgcttggttc gacgagtcga ggacgttgag cgagccgttc tggacggcga ggaactgcgc
    17221 accatcgggc gtgaacgcgc cgaagttcca gccgcgcgtc gacgcctgca cggccttcgt
    17281 accgacgtcc atgagcgtgg cggcgccgtt gccaccgtca tacgtgatcg acatgcgcgt
    17341 gccgtcgcgc gagagaacgt ggcaagcgac gcagcggccg ccggtctgcg ccgtcgtcat
    17401 gaactgctcg gcggcttggc cgggcttgcc catgtcgtgg cggaagatgc cgtacgtgcc
    17461 gttctgcgcg gtcgacgccc agtagtagac gccaccgagc atcgcttcgt tgctgagctt
    17521 gacgagctgc ggagcgcccg caccgacgac cgtcgggttg ttcttgtcga cgccgcgcac
    17581 ttggaattgc accgaggtct cgtagccgac ggcggcgagc cactcgttcg cgaggaacgc
    17641 gagccacgag ccgctcgcga tggcgccgtt gccaccgggg acaatcgcgc ggacatcagt
    17701 gaactcggtc ttgagcgaaa cctcgaacac gttgttcgcg gtgtccgtcc agtgaacttc
    17761 gaagtcgccg aggttgcgcg gcatgacgac gttcgccggc ggatacacga ccgtcggcgc
    17821 gcgcgatgga tcttcggggc tcgtgaacca gttcgctgca tcgggcggca gcgacggatc
    17881 gatgcggatg tctttgaggc ggatgatgac ttgcgacgcg gccgtcttgt cgctgagcga
    17941 tgcgaagact tgcgtcttgc ccgccgtcgc gaacgtgccg gtgtggccgc tcatcattcc
    18001 gtagccgttg tcgaccgcga agtgcgcttg gtcggtgacg tccttcgttt caccgtcggg
    18061 atacgtcagc actgcagtga agtccgcagt cgctggtgtg ccgtcgagga tgaggagctc
    18121 cgccgtcggt ggatcgacgg tgagcaccgc atctttgtcg tcgtgatcac ctcccgacgg
    18181 accgcagcca acgaacgcca aaaccaacgc gagcgaaatg cgctgaaccc tcatcgaggc
    18241 gcacattacg aaggttttat gccgctgaga agatgccgaa atttggcatt cagctgggga
    18301 cagctgactc cagcacgcct tgtggtaagc acgctcgcgt gcggacactg acttccgtct
    18361 tgggaaatgg gcagcgcctc gacggcggcg ccatgttcgg taacgcgccc aaggccatgt
    18421 gggaaaagtg gattccgccg gatgacaaga accggatccc gctcgcttgc cgctgcctcg
    18481 tggtgcggga cggaacgcgc acgatcctgc tcgaagcggg catcggggcg ttcttcgagc
    18541 cctcgttacg tgagcgttac ggcgtggtcg aggaccgcca tgtcctgctc gagtcccttg
    18601 cggcgctggg tatccggcct tcggacgtcg atgtcatcgt gctctcgcat ctgcacttcg
    18661 accacgccgg cggcgtgctc tcgacgtggc agcaaggcgc gcagccgtcg ctcgtgtttc
    18721 cgaacgcgaa gtatgtcgtc ggtgccgagg catggcagcg cgcgatccag ccgcacgcac
    18781 gcgatcgcgc atcgttcatc ccgggcctca ccgacatgat ggccgcgacg ggtcgcctcg
    18841 agatcgcggc cggcgaccgc tccgacgtgc tcggcgacgg ctatcgcttt catcgcagct
    18901 cgggtcacac gcccgggttg ttattgaccg aggtcgagat ggccgacggc cccgtcgtgt
    18961 ttgccgcgga tctgatcccg ggcaaggcgt gggtgcacct gccgatcacg atgggctacg
    19021 accgctatcc cgagatgctg atcgatgaaa aggccacgtt gctcggcaac ctgctcgagc
    19081 gacatggccg gctgttcttc acgcacgatc cggtggtcgc gatgggcacg gtcgctaaag
    19141 acgagcgtgg acggtttcac gtcgtcgacg agcgcgaaac gatccgcgac ctcgctgcgt
    19201 agctcagccc tcgcccgcga gccgatcgag tgcggtgttg aaggcgcgat cgtcgtggca
    19261 tgccgccatc gtggggccct gcatgatcgc ctgcgcagct ggatcgatct cggcgtcgtg
    19321 cttctcgagc tccgcgcgtg cggctttgat cttctcgtgg ccggacttcg tgaggcgctg
    19381 attggcagcg atcagatcgg cattcgcatc ggcgagcgca ttcatcttcg tcgtcgcggc
    19441 cgcgcagtcg gtaccggctt cggcaaacgc cttctgccag tcttggtaca tcttcacggc
    19501 acgctcggcg agcctcggca gatcgtcttc gaggggacct tgctgcgcag gcggtgcgtc
    19561 caccgcgcta ccggaggccg gtgctgcgac aggcgttacc ggagacgcgc tcggatggcc
    19621 gcacccgatc gcgatagccg caagccacgc gagtctctca gtgcgtgcca tcggcggctc
    19681 gcatcttctc gaggactacc tcgatcgtcg ccgcgtcgac attcgccctc cgcatcagcg
    19741 acacggcacg gcgctcgtca tcggcatcga tcgcctcgaa gaactcgtcg accttctcga
    19801 tcgcggcgag ttgctcgagc acgagcgtgc cctcgaactg cgtctcgtcg atctcgctca
    19861 cgtgctctcc gcctcgagct tgcggcggac cttctccatt tcggctttct gctccggcga
    19921 caccgtcggg taatcgatct cgagctgcgc gagcgtgtcg acgatcgtct cggcgacgat
    19981 gcgccgcatg tagctcttgc tgtcggcagg gatgcagtac caaggcgccc acggcttgct
    20041 cgtcgcgttg agagccgcgt tgtacgcctt ctggtaatcg tcccagtgct cggactcctt
    20101 catgtccgac gccgagaact tccagttgtg ctcgggatcc gtgatgcgcg cgaggaagcg
    20161 ctcgtgctgc tccttctgcg acacgttgag gaagaacttc agaatcacgg cgccgttgcg
    20221 cgcccagtgc cgctctgcct cgttgatcga gtcgaagcgc tgttcccaca gctcctcgag
    20281 atccttcggc atgtacggca agcgctggcc ctcgagatat tcgggatgca cgcgcacgac
    20341 gaggacctcc tcgtagtgtg agcgattgaa gatgccgatg cgaccgcgct cgggcagcgc
    20401 gcactggacg cgccagagga agtcatgatc gagctcgagc gacgacggct gcttgaacgc
    20461 gtagacctga cagccttgcg ggttgatgcc cgacatgacg gccttgatcg ttccgtcttt
    20521 gccggcggcg tcgagcgctt ggaagatgag gatgaccgag tagcgatcct gtgcgtagag
    20581 cttgccttgc agcttcgcga gcctctcgac gtactcttgc agcgcggcga cgttttcttt
    20641 tttggccggc ttgttctccg gctcggtcgc ctgcttgttg agcttgaacg agccgtcgaa
    20701 cggaacgaga tacggattct cgaccggctt gaagaggatc ggtttcggca ttgcgctcgg
    20761 acgataaacc ggttgcgcgg caatccggag ccacgggtgt atggtccgcg aactatggcg
    20821 acgactacga cgacggacgg ttcgaaaacg gatgtggtgc cggcgttctc cgaggaggac
    20881 aagctcggcc cgctcggcgg tgggaagcgg tctttgctcg acgacgaagc cgagctgcgg
    20941 cgcgtcctcg tcgtggagtt tcaggcggcg gccgatgcgg cgcgcgatgc cgcgggcagc
    21001 gtcgacgaga acgtgacgac cgccgtccat gattttcgca aggcgttgcg ccgtgcgcgt
    21061 gcggtgctgt cgctgaccgc ggctgcgctg ccgaagagcg agcgtcgcgc gatcaatcgt
    21121 gcgttgcgcg aggcgcggcg tgcgctcgga accgcgcgcg atcacgcggt cgcgccggac
    21181 accgtcgatg tgctgccgct cggcgatggc gagcgcgact cggccagctc gatcattcgc
    21241 gccgctgctg actcgatgcc gccggtcgcc gagatcaagc aactgctcgc cgaaggcgcg
    21301 gcgcgtgctg cggcgcaagt cgaggcgctc gaggccgcac tgccgcagac cgtcgagtgg
    21361 tcgaccgtcg agaagggaat ccgcagcacg tatcgcgatg cgcgcacggc gcggaagagc
    21421 gcgaagcgca accggcgttc gttccatgcg tggcggcgcc gcagcaagga gcttggctac
    21481 cagctcgagc tgctcgcggg ctacgcgggc acgcgcgcga ccgagctgtc acgcgagatc
    21541 gaaagcgtga cggacacgca gggtcccgcg gtggacctcg tgatgctgcg cgacttcgtg
    21601 cgtacgcatg ctgcgggagt cgagtcggag tccgtgcatc ggctcgtcag ctcgatcgaa
    21661 gcgcagatcg acgatctcgt cgccgattcg cgccgcgctg gacgcgaagc gttccgcaag
    21721 aaaccgcgcc ggtttgcgcg ccgcctgacg aaggccgtgc gacgcgatct cgcgccggtg
    21781 gtcgttcccg acgacgtcga ctgacgctat aagagggcca atatgtcgaa ggtcctcatc
    21841 atcggagccg gtggcgtcgg cggcgtcgtc gcgcacaagg cggcgatgga gaagcagcat
    21901 ttctctgaag tcatgctcgc gagccgcacg ctctcgaagg tcgagaagat cgccgccgag
    21961 gtcgagcaga tgcacggccg caagatccag acgcggcagg tcgacgcgga caacgtcccc
    22021 gagctcacga agctcctgaa ggagtacaag ccggccgtcg tcatcaacgt cgcgctgccg
    22081 tatcaggacc tccacatcat ggatgcgtgc ctcgaagccg gcgtcgatta cctcgacacc
    22141 gcgaactacg agccgcccga tgtcgcgaag ttcgagtaca gctggcagtg ggcgtatcag
    22201 gaccggttca agaaggccgg ccggatggcg ctactcggct cgggcttcga tccgggcgtc
    22261 acgaacgtgt tctgcgcgta cgtgcagaag cacctgctcg acgaggtgca ctacgtcgac
    22321 atcgtcgact gcaacgcggg ctcgcacggc aaggcgttcg cgacgaactt caatcccgag
    22381 atcaacatcc gcgagatcac cgcgcggggc cgctactggg agaacggcga gtggaaggag
    22441 accgatccgc tcagcgtgtc gaaaatcttc gactacccgg gcgtcggcga gaagaagagc
    22501 ttcctcctct atcacgagga gctcgagtcc ctcgcgaaga acttgaaggg cctgaagcgc
    22561 atccgcttct ggatgacgtt tggcgaggcg tacctaaagc acctcgaggt gctgcagaac
    22621 gttggcatga cgcggatcga cccggtgctc tacaacggcg tggaaatcgt gcccttgcag
    22681 ttcctcaagg ccgtgttgcc cgagccttct tccctcggcc cgaccacgaa gggcaagacc
    22741 aacatcggcg acatcgccac cggcgaagcg ctcgacggtt cgggcgaga
//
LOCUS       DATLBZ010000035         2513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8968256, whole genome shotgun sequence.
ACCESSION   DATLBZ010000035 DATLBZ010000000
VERSION     DATLBZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8968256"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VMZ53_01790"
     CDS             <1..1141
                     /locus_tag="VMZ53_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS27200.1"
                     /translation="KDQAIKFINALHAGGGTDIALALKTGIKSQEAKSENPKVIMFMT
                     DGQSDAELAMDAAKADTKDVRVFTIGLGKEVNKPLLSRLAAVKRGRFTYIETAASIEP
                     EVRRLASSIAKPLLVNVEVEVEGATAMRMYPRTLPDLFAEDELLVTGRMRGKGSAKFI
                     IKGKLAGKDVTYTRTVDLSKAAARPWVGGLWAQSRIDHLLEEIALGNKDDETKTEVLE
                     LALAYNVVTPFTAFLAIPESELGNMQGTVDQARAQKQKILAQNPDIAGLKKEKNADPA
                     DLAANQVAQGAPPPKATTTQSFAMDGADDESSINGAPPSPDMAPREYASMDRGGDEES
                     EDDSEPVRANGSVKQHGCAGCASTGGDAGLFALVFVALVLRRRRR"
     gene            complement(1138..1932)
                     /locus_tag="VMZ53_01795"
     CDS             complement(1138..1932)
                     /locus_tag="VMZ53_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27201.1"
                     /translation="MRTGLILLSAAVAATPAAAETRLKHHTPGVQEDEINTKAEVEPT
                     VAAGTLLPWSIGARHERQGILLGGYGGADLAKRAPVMAGTLDATLIDRVTLHATMTNV
                     GMSNMLRPNVGLLVDVMRTEDTGVDLAVGGDYDIVGFNGVPAVVARTAIGANAGSLRL
                     QGNAGFGLGLEQNERFGQVGISGLTPVAKALYVGIDSRARMDLERDDMEPSGELDWDM
                     QAGPVATLALGRFAVSATGGVSAWKQRTHDTAKVGAVGAIGVGAAF"
     gene            complement(1992..>2513)
                     /locus_tag="VMZ53_01800"
     CDS             complement(1992..>2513)
                     /locus_tag="VMZ53_01800"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HUS27202.1"
                     /translation="HVPFKLEPQAPGKLDVEAAHRLLDRIINEEPMALECRDAPAGFG
                     HMVAEKLLAKDRSARYQTMKEVQGALAAFAGVAATPVVLASAPQSPEVQQLGSRWRVA
                     SGDLVLELFSDEMHELADVVTAAAAIADEMKHYPMIALELPRLRVTLSSAAAGTDLVY
                     AARLEQWLRDHGW"
BASE COUNT          461 a          825 c          844 g          383 t
ORIGIN      
        1 gaaggatcag gcgatcaagt tcatcaatgc gctgcacgcc ggcggcggta cagacatcgc
       61 gctcgcgctc aagaccggca tcaagtcgca agaagccaag agcgagaatc cgaaggtcat
      121 catgttcatg accgacggcc agtccgacgc cgagctcgcg atggacgccg cgaaggccga
      181 tacgaaagac gtacgcgtgt tcacgatcgg actcggcaaa gaggtcaaca agccactgct
      241 gtcgcggctc gccgcggtca agcgcggtcg ttttacctat atcgagaccg ccgcatcgat
      301 cgagcctgaa gttcgccggc tcgcgagcag catcgcgaag ccgctcctcg tgaacgtcga
      361 ggtcgaagtc gaaggcgcga ccgcgatgcg gatgtatcca cggacgctgc cggacctgtt
      421 cgccgaggac gagctcctcg tgaccggccg catgcgcggc aaaggcagcg cgaagttcat
      481 catcaagggc aagctcgcag gcaaagacgt cacgtacaca cgcacggtcg acctgtcgaa
      541 ggccgcggca cgtccgtggg tcggcggcct gtgggcccag tcgaggatcg atcacctgct
      601 cgaggagatc gcgctcggca acaaggacga cgagacgaag accgaagtcc tcgagctcgc
      661 gctcgcctac aacgtcgtga cgccgttcac cgcgttcctc gcgattcccg agagcgagct
      721 cggcaacatg cagggcacgg tcgaccaagc gcgcgcgcag aagcagaaga tcctcgcgca
      781 gaacccggac atcgccggcc tcaagaagga gaagaacgcc gaccccgccg acctcgcagc
      841 gaaccaggtc gcacaaggcg cgccgcctcc gaaagcgacg acgacgcaga gcttcgcgat
      901 ggatggcgcc gacgacgagt cctcgatcaa cggtgcaccg ccgtcacccg acatggcgcc
      961 gcgcgagtac gcgtcgatgg atcgcggtgg cgacgaagaa agcgaagacg atagcgagcc
     1021 ggtcagggcg aacggctcgg tgaagcagca tggctgcgcg ggctgcgcga gcaccggcgg
     1081 tgacgcgggg ctcttcgccc tcgtgttcgt cgcgctcgtg ctgcgccgcc gccgtcgcta
     1141 gaacgccgcg ccgacgccga tggcgccgac cgcgccgacc ttcgcggtgt cgtgcgtgcg
     1201 ctgcttccag gcagagacgc cgcctgttgc gctgaccgcg aagcgaccga gtgcaagcgt
     1261 cgcaacggga ccggcctgca tatcccagtc gagctcgccg ctcggctcca tgtcgtcgcg
     1321 ctcgaggtcc atgcgggcac gcgagtcgat gcccacgtag agagccttcg cgacgggcgt
     1381 caggccggag atgccgacct ggccgaagcg ctcgttctgc tcgagaccga ggccaaaacc
     1441 ggcgttgccc tgcaggcgca gcgagccggc gtttgcgccg atggcggtac gcgcgacgac
     1501 cgcggggacg ccgttgaaac cgacgatgtc gtaatcgccg ccgacggcga ggtcgacgcc
     1561 ggtgtcttcg gtgcgcatga cgtcgaccag gaggccaacg ttcgggcgca gcatgttcga
     1621 catcccgacg ttcgtcatcg tggcgtgaag cgtgacgcga tcgatcagcg ttgcgtcgag
     1681 tgtgcccgcc atcacggggg cgcgctttgc gaggtcagcg ccgccgtaac caccgagcaa
     1741 gatgccttgg cgttcgtggc gtgcaccgat cgaccagggc aggagtgtgc ccgccgcgac
     1801 ggtgggctcg acctctgcct tcgtgttgat ctcgtcttct tgcacgcccg gcgtgtggtg
     1861 cttgaggcgc gtttccgcag cagcaggcgt tgccgccaca gcggcgctca gcagaatcag
     1921 tccggtacgc atgcgtacga aagactgcga ggcacgtgcc aggcggtatc acgcgcagcg
     1981 agcgtaatcg ctcaccaacc gtggtcgcga agccactgct cgagccgcgc ggcatagacg
     2041 agatccgtcc ccgcggcggc cgaagagagc gtgacgcgca ggcgcggaag ctcgagcgca
     2101 atcatcgggt agtgcttcat ctcgtcggca atcgcggcag ctgccgtgac gacgtctgcg
     2161 agctcgtgca tctcgtcgct gaagagctcg agcactagat ctccgctggc gacgcgccag
     2221 cgactgccga gctgctgtac ttcaggcgat tgcggcgcag atgcgagcac gaccggcgtc
     2281 gcagcaacac ctgcgaacgc ggcgagcgcg ccttggacct ctttcatcgt ctggtaccgc
     2341 gcgctgcgat ccttcgcgag cagcttctcg gcgaccatgt ggccgaagcc tgcgggcgcg
     2401 tccctgcatt cgagcgccat cggctcctcg ttgatgattc gatcgagcag gcgatgcgcg
     2461 gcttctacgt cgagctttcc cggagcttgt ggctcgagct tgaacggcac gtg
//
LOCUS       DATLBZ010000036        14178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8968506, whole genome shotgun sequence.
ACCESSION   DATLBZ010000036 DATLBZ010000000
VERSION     DATLBZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14178
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8968506"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VMZ53_01805"
     CDS             <1..845
                     /locus_tag="VMZ53_01805"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HUS27203.1"
                     /translation="AMLETLLDYLREPLVVLSGTQISALTLLTAILIIVVAKIASVVI
                     GRSIERVLEVRDVDKGVRFAAGRIARYVVIVIGMFVAIGTLGIDTSAMMGAAAVLLVG
                     IGFGLQKLAENFISGLLVLIERPVRKGDFIDAGGVLGVVDDIGLRATRVTSRDGVQVI
                     VPNSHLMTNIVVNYSQPSHERRFQVDIGVAYGTDLELASKVILDVAKAEPKVLGTPAP
                     EVRHMGFGDSSIHLILLIWIQEAKEDLIVGSRLRFALDKAFREHSIKIPFPQRDVHMT
                     TRVA"
     gene            842..2140
                     /locus_tag="VMZ53_01810"
     CDS             842..2140
                     /locus_tag="VMZ53_01810"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HUS27204.1"
                     /translation="MKRRPAPPSRPSSLSAADARGIALVAQQFHGAARSLHAPANRVD
                     HKSIRSLISHLGVVQIDSVNVLVRSHYLPAFSRLGAYTHSDLDRLSHEHPRSLFEYWG
                     HEASLLPVALQPFFRWRMAQAEEKAWGRIRRMAKKNPTFIASVLDTIRERGPLTASQL
                     EPEMEQAGLLPKRTGRKKSGWWEWSDTKTAIEFLFWSGQVTSSGRRGFERIYDMPERV
                     IGKAILAQPTPTERDAHRYLLDRAGRGLGIGTEADLRDYYRLPVAGARAALADAVESG
                     TFERVSVEGWAKPAYLHKSAWIPSGDEIDPDRGALLSPFDSLIWFRDRTERLFGMRYR
                     IEIYTPAPKRVHGYYVLPLLLGDELVARVDLKADRAAGILRVQAAHAERTRKRGVTKA
                     RVTTALAGELSRMASWLGLAGIEVMPRGDLAASLARLKIR"
     gene            complement(2127..2477)
                     /locus_tag="VMZ53_01815"
     CDS             complement(2127..2477)
                     /locus_tag="VMZ53_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27205.1"
                     /translation="MGTLSKRRLEDFGVDVKGRVERFRSNVGRRLDDMHHDVELKGRR
                     EGALTRALEKISAALPSTTWLAFAGASAVTSVALQLTRKKHGALFVATWVPSFLLLGV
                     YNKLVAASDRINES"
     gene            2651..4426
                     /locus_tag="VMZ53_01820"
     CDS             2651..4426
                     /locus_tag="VMZ53_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27206.1"
                     /translation="MARWFAFVVALLFTSPAFADDPADVAFDPTIDECAAPDAHCLTD
                     SGEPTDAELEAQEMALAEDVVPSTWLAAPADVACPSSSSLQDGRCVPDGTATVDIEGG
                     CNASRTTGLVMVLALSGIIVWARRRRALLLVLAACALDSGSYDDAVDEGATGDSTFLD
                     VYSTDAGDGTVFLLAHQAPPAGMPEPVAQFSLMREHAALPLFRASTTCGDHLVTVAGD
                     DTELLGWARDTAGEGTADLVELENAAGCFAYEIDQEAIDALVADGFHVAGRVGYVWPP
                     GLSDPPPPPEPLTDDPIAANGPAACPNVNRTSALQLLYASPGKEETLRFLKGCPGEVI
                     VGEKRETGPVGSMKSIEAHAAGGRTAFVLDRHGDKLRELLLRDNGVERTVAYLKKKMQ
                     LGYDYIVIDEITASADFRDGTTLNRRLRKLLLRMPARTIIPYISIDLTQQTYGFAAMQ
                     GRKLLLRAFKLRARSIALEVYLHTAQVRAGAAPATFRRAADRLALAVRGLSKTAGINR
                     RAITVIGTSMHSSYPQYRYLDQASYDLASLSRQVNALRHGSKRLRQQKGVGWYFVNKS
                     DMAPPSTYSYDALIRRMRLLGLRFK"
     gene            4601..5911
                     /locus_tag="VMZ53_01825"
     CDS             4601..5911
                     /locus_tag="VMZ53_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUS27207.1"
                     /translation="MHDEKVQRIAAQLRSHDGSKPVSLRKKAPPHQVPKGGDLRRKDD
                     KIDVSDLTSIISVDPISRTAVAESGVMFCDLVEATLRHGLVPIVVPELKTITIGGAVS
                     GCSIESMSYRYGGFHDTCLEYEVVTAQGEVLTVTPSNEHALVFQMIHGSFGTLGVLTK
                     LTFKLVPAKPFVRVDYERYHNIKDYQAAIQRHYKAQDVDFMDGIIHRPNLMVLSVGKF
                     VDKAPYTNAYDWTKVYYASTARLDHDYMKTPDYFFRYDRGVTNVHPKSFIGRLFLGKI
                     LDSGTTLRLADKLNFMFKTDRPTVILDVFIPFSAAPAFMEWFEKEVGHFPLWCVPYKR
                     VHDYEWLDNSFYANMKDELFLDLAIYGMEQPEGKNIHRMIEEKLRELGGVKTLISHNY
                     YSKDEFWQIWNKDNYEKVKAVTDPKNVFRDLYTKTCRAAMGIAG"
     gene            5927..6778
                     /locus_tag="VMZ53_01830"
     CDS             5927..6778
                     /locus_tag="VMZ53_01830"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HUS27208.1"
                     /translation="MGRFLAAVFVLGLTSTAFAQGPRTGGPVPGTVQGAKASVDLSLT
                     GNLARGISDRDLVIGRGNLQYWTGPWGFFLQPYYLYGDVKLGAMPRVKTDDERYLRAS
                     VFRTLVKPMFTYGVVALDHSLRRRIDRRALAGGGVGVTLTNQADITFVTSVGLLYEHG
                     EYGEGKVQDQIFPAKNRNVVRTSLRLYGIYKLPHVNLIHDVYFIPNVRDLHDLRAMFS
                     GVLEVPVAKGFAGRVSVDATHEGVIVEGTQEDDIAITFGVSFKNDWTFDPPPPPPAPT
                     PGAPAMP"
     gene            complement(6750..7373)
                     /locus_tag="VMZ53_01835"
     CDS             complement(6750..7373)
                     /locus_tag="VMZ53_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27209.1"
                     /translation="MLPPLFANLPSVFVSHYDPETPWTLLGDALDTILAGLDLIGEGT
                     RVHPSAVIEGPVFIGKHVDVRPGAYIRGGSWIGDYCVVGTNTEIKRGILFPGAKASHL
                     NYVGDSILGANVNLAAGTVLSNYRHDAASIQIAGTNTQRNKLGAILGDGVLTGCNSVL
                     HPGCVVGRGTQIYPGVQLRPGVYPAQSIVKLKQELEIVARASPAHPA"
     gene            complement(7388..7549)
                     /locus_tag="VMZ53_01840"
     CDS             complement(7388..7549)
                     /locus_tag="VMZ53_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27210.1"
                     /translation="MNESADPQHRTTREMKAVSKKDTGRERQVMDERCVAARRWIDVD
                     DEPAIRGID"
     gene            7679..7912
                     /locus_tag="VMZ53_01845"
     CDS             7679..7912
                     /locus_tag="VMZ53_01845"
                     /inference="COORDINATES: protein motif:HMM:NF023238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HUS27211.1"
                     /translation="MKKLLAPLVLSAVLSGCYGSYSASHALHKANGHIGSPIANSAVH
                     LVLWIIPVYPIVYFGDFLIFNNIEFLTNKPVFN"
     gene            8017..8637
                     /locus_tag="VMZ53_01850"
     CDS             8017..8637
                     /locus_tag="VMZ53_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HUS27212.1"
                     /translation="MMKILSRVLVTALLTVSFAASADDTTTKTQPSDAPKTAETPAKP
                     AKPAKLKQAELQVMAHYHAVNKMEIDLGNAAAKKAMTDAVKEYGKMLVKDHGESDKAL
                     LALAKKTGQVIPAEKPADDIEKKEKADDKAQAAKLKTLKGADFEREYLRMMVQGHEKE
                     LGKIDANIAKVENAELADALKAKKPVLQHHADSARELQKNEPTASR"
     gene            8846..9712
                     /locus_tag="VMZ53_01855"
     CDS             8846..9712
                     /locus_tag="VMZ53_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27213.1"
                     /translation="MKPLAICLFVTATAACTADVEIITEDQEATPGASITTERSLSGC
                     NGHAATSIPADGRYVITTFGGPGDHQPMSCGGYADGTGWYAASRQRYGCGSHIKVQAN
                     GKCVVLKTDDYGPDVCVETAAHMPIIDVTPMASKALFGASGAGWSDHLVVTVEEVATS
                     VPLGPCTATPPGGGGGGTMNPPAGSASCASATLDRDVEDGVCVQSATDSLWYACNNGT
                     WAARSSSAGCAEAYGFCNSATLGKNVPARTCVQAASNSVWYQCNGQGWVTPVSTSAKS
                     GPLGDCATWNPL"
     gene            9789..11003
                     /locus_tag="VMZ53_01860"
     CDS             9789..11003
                     /locus_tag="VMZ53_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS27214.1"
                     /translation="MFRLDEQSQMVATMVRGWCTSQLAPKIPALEHGTEMPFDLMKKM
                     AKAFGFDAMLGASVQKRIAKLRAGEDTTASAEKTLAAGVGNPMMMHVVVKELSRVSPG
                     FAMGFGVSMGLVGGAIVAKGTADQIEKWGLPVATVQQIGSWCLTEPNAGSDAFGSMRT
                     TARKEGSDYILKGSKTFITNGPGADIFLVYAKTEDGGVATFIVERNMPGVSVGPPFKK
                     MGMRDSPTSEVFFDDVRLGVEHLLGGKERGKAGRGDTKDSLGSERSGVPAMAWGIIER
                     CYDASLAYVREREQFGRRIGEFQAVQLKIADLFLKLRTVENVVFRTSWMQQNGVRDMP
                     FVNASKAMCAQMAVDAALTAVQIHGGYGYMEELGIEKLARDAKLLELGAGTTDINLLA
                     CARELIGVHGNE"
     gene            10990..11361
                     /locus_tag="VMZ53_01865"
     CDS             10990..11361
                     /locus_tag="VMZ53_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27215.1"
                     /translation="MATSRLSLLVLTLAACTPNVPEKKPPKGALIETLAAGSNAVVVE
                     TIEPAASASPEALRQYLLSKAPIDHRAALADGFTATFGGETHVLRERHNRWYRCHAPV
                     TDDAMRDDVIAVCKSYKLPAQ"
     gene            11358..12974
                     /locus_tag="VMZ53_01870"
     CDS             11358..12974
                     /locus_tag="VMZ53_01870"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HUS27216.1"
                     /translation="MILAPLAIAAGLDVGRVVDNGQIHENRALVDATGIGWVRINLRL
                     DTKSAIEDEFPIYDHVIDDYLARGISVYILINDEAVSSTLAHGSDAWIEQAFVPAAVK
                     IVDHFKNRVRVYEIINEPNDFAGGSSARFSPRQFAKILQDTYLAVKHDGGHDADACWQ
                     VALNSGPLFSFDDTPADTYLDQTYAIGRSQLAWDYTHQTTGRYPLDGIGYHMYVAQGL
                     DSSNADVRTQMLANLSAINAVVTARDDATKPLWVSEYGWEASVVGNDVQAQRLTAGFS
                     AMAEAGTVATAFYFNFQDFPGATYGIYDDALAVRPSAAALSQIAPLPLRASIEQVIVP
                     PTIAPGADADIVVTLRNRGTETWTPQAGYRLAAAAGCPDASAANEIAWNPAATDGYAT
                     SPIDARIYLQHSVAAGDLVEIHVPIHAPETTGSYTFAARMVQEGVSLFGPTIRATITV
                     ANAPVDGDDTSTTPPPTSPGGCCSSTTSNSSTTLLSLVVSVGVLLLRKRRRGTSAAAS
                     RVRRFSYAVDSLSPRRLSRRARTRDRGMHR"
     gene            12901..13536
                     /locus_tag="VMZ53_01875"
     CDS             12901..13536
                     /locus_tag="VMZ53_01875"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HUS27217.1"
                     /translation="MLSTRFLLAASLAVLAPATAACTDSSTTGVVVDDPVGDTIDEGR
                     ATGTTIAAQAFGELSADDYQTMIGKTGELLATLHDGEIQQASFAIQVVQDDDVFDYAN
                     NMIIEHEDGLALLDDTMRFYGAPYIPSQAQAQLMADAQAALADLRATPPQDIDFRYME
                     VQVRMHASAQVILDQLSQLVGPGAMNDYIINARGMVDEHFDRATSILETYY"
     gene            complement(13530..13769)
                     /locus_tag="VMZ53_01880"
     CDS             complement(13530..13769)
                     /locus_tag="VMZ53_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27218.1"
                     /translation="MDLGAMTLAGFLALSQVAKAIGGCEGAGGVECGEAALRNAAIIA
                     GVGGVFFVSAIYGFAAKSSCHAKAKAAVSASGGTQ"
BASE COUNT         2601 a         4822 c         4374 g         2381 t
ORIGIN      
        1 aggcgatgct cgaaaccctc ctggactacc tgcgcgaacc gctcgtcgtg ttgtcgggca
       61 cgcagatcag tgcgctgacg ctccttacgg cgatcttgat catcgtcgtc gcgaagatcg
      121 cgtcggtcgt gatcggccgc agcatcgagc gagtcctcga ggttcgcgac gtcgacaagg
      181 gtgttcgctt cgccgcgggt cgcatcgcgc gctacgtcgt catcgtcatc ggcatgttcg
      241 tcgcgatcgg tacactcggc atcgacacga gcgcgatgat gggcgcggcc gctgtcctgc
      301 tcgtcggtat tggcttcggt ctccagaagc tcgccgagaa ctttatctcc ggcttgctcg
      361 ttctgatcga gcgtcccgtg cgcaagggcg acttcatcga tgcgggcggc gtgctcggcg
      421 tcgtcgacga tatcggcctc cgcgctactc gcgtcacctc gcgcgatggt gtgcaggtga
      481 tcgtgccgaa ctcgcacttg atgacgaaca tcgtcgtcaa ctactcgcag ccgtcgcacg
      541 agcgtcgctt ccaggtcgac atcggcgtcg cgtacggcac cgatctcgag ctcgcctcga
      601 aggtgatcct cgacgtcgcg aaggcggagc cgaaagtcct cggaacgccc gcccccgagg
      661 tccggcacat gggctttggc gacagctcga tccatctcat cttgctcatc tggatccagg
      721 aggccaaaga ggacctcatc gtcggcagcc gcctgcggtt cgcgctcgac aaggcgttcc
      781 gcgagcacag catcaagatc ccgttcccgc agcgcgacgt tcatatgacc acgcgcgtgg
      841 catgaagcgt cgcccggcgc cgccgagccg cccgagctcg ctctcggccg ccgatgcccg
      901 tggcatcgcg ctcgtcgcgc agcaattcca tggggccgcg cgcagccttc acgcgcccgc
      961 gaaccgcgtc gatcacaaga gcattcgatc gctcatctct caccttggcg tcgtgcagat
     1021 cgactcggtc aacgtgctcg ttcgatcgca ctacctcccc gcgttctcgc gactcggcgc
     1081 gtacacgcat agcgacctcg atcgattgag ccacgagcac ccgcgatcgc tgttcgaata
     1141 ctggggacac gaagcatcac tgctccccgt cgcactgcag cccttctttc gctggcggat
     1201 ggcgcaggcc gaggagaaag cgtggggacg cattcgccgg atggcgaaga agaacccgac
     1261 gtttatcgcg agcgtgctcg acacgatccg cgaacgcggc ccacttaccg cgagccagct
     1321 cgagcccgag atggaacaag ccggcctctt accgaagcgc acagggcgca agaagagcgg
     1381 ctggtgggag tggtcggata cgaagacagc gatcgagttc ttgttctgga gcggccaagt
     1441 cacgagctct ggacggcgtg gcttcgaacg catctatgac atgcccgagc gtgtcatcgg
     1501 caaggcgatc ctcgcgcagc cgacgccgac cgagcgcgac gcgcatcgct atctcttgga
     1561 tcgcgccggc cggggccttg gcatcggcac ggaggccgac ctgcgcgact actatcgact
     1621 gcccgtcgca ggcgcgcgtg ctgcgctcgc cgatgccgtc gagagcggca cattcgagcg
     1681 cgtctccgtc gagggttggg caaaaccggc gtacctccac aagagcgcct ggattccaag
     1741 cggcgacgag atcgatcccg atcgcggcgc cctgctctcg ccgttcgact cgctaatctg
     1801 gtttcgcgat cgcaccgagc ggctcttcgg catgaggtac cgcatcgaga tctacacgcc
     1861 ggcgcccaag cgcgtgcatg gctactacgt gttgccgctc ttactgggcg acgagctcgt
     1921 cgcgcgcgtc gacctgaaag cggatcgcgc cgccgggatc ctccgcgtgc aagctgctca
     1981 tgctgagcgg acacggaagc gcggcgtgac gaaggcgcgc gtgaccacgg cgctcgccgg
     2041 ggagctatcc cggatggcgt cgtggcttgg actcgcgggc atcgaggtca tgccgcgagg
     2101 agatctcgca gcatccctcg ctcggctcaa gattcgttaa tgcgatcgct cgccgcaact
     2161 agcttgttgt acacgccgag caacaggaag ctcgggaccc acgtcgcgac gaacagcgcg
     2221 ccgtgcttct tgcgtgtcag ctgcagcgcg acggacgtca ccgccgacgc acccgcaaat
     2281 gcgagccacg tcgtcgacgg cagagccgcc gatatcttct cgagcgcacg cgtcaacgcg
     2341 ccctcacgcc ggcccttcag ctcgacatca tgatgcatgt cgtcgagcct gcggcccacg
     2401 ttcgagcgga agcgttccac gcgccctttg acgtcgactc cgaaatcttc gagccggcgc
     2461 ttactcaagg tacccatgtt tcctccttcc ccattttggt gcagcgcatg tgccaccgac
     2521 ggacaagtgc ccacggccat gtgcacgagc aagtgcgcgg tttcgtaaag caaggcagct
     2581 tcagttcccg tacctcggtg aattgccttg ctgggcgctg tggcacgaac cgagcacttg
     2641 gcgcgccgcc atggctcgct ggttcgcctt cgtcgtcgct ctgctcttca cgtcgcccgc
     2701 cttcgcggac gatccagctg acgtcgcctt cgatcccacg atcgacgaat gcgccgcgcc
     2761 agacgcgcac tgcctgacgg actccgggga gcccaccgac gccgagctcg aagcgcagga
     2821 gatggcgctc gccgaagacg tcgtgccgtc gacgtggctc gcagctccag cggacgtcgc
     2881 ctgcccttcg agctcctcgc tgcaagatgg ccgctgcgtt cccgacggca ctgcgaccgt
     2941 cgacatcgaa ggcggctgca acgcttctcg cacgaccggc ctcgtgatgg tgctcgcgct
     3001 cagcggcatc atcgtgtggg cacgacgccg gcgtgcgctc ctcctcgtgc ttgctgcgtg
     3061 cgcgctcgac agcggcagct atgacgacgc cgtcgacgaa ggcgcaactg gcgactcgac
     3121 gttcctcgac gtctactcga ccgacgcggg cgatggcacc gtgttcttgc tcgcacacca
     3181 agcgccgcct gcgggcatgc cggaacccgt cgcgcagttc tcgctgatgc gagagcatgc
     3241 tgcgcttccg ctgttccgcg catcgacgac gtgcggcgat cacctcgtca ccgtcgcggg
     3301 cgacgacacc gagctcctcg gctgggcgcg cgacaccgca ggcgaaggca ccgccgacct
     3361 cgtcgagctc gagaatgcag ccggctgctt cgcgtacgag atcgatcaag aagcaatcga
     3421 tgcgctcgtt gcggacggct ttcacgtcgc cggacgcgtc ggctacgtgt ggccgccggg
     3481 gctctccgat cctcctccgc cgccggagcc gctcaccgac gatccgatcg cagccaacgg
     3541 tcccgccgcg tgcccgaacg tgaaccgcac gtccgcgctg cagctcctct acgcgagccc
     3601 cggcaaagag gagacgctgc gcttcctcaa gggctgcccc ggcgaggtta tcgtcggcga
     3661 gaagcgcgag acgggtcccg tcggctcgat gaaatccatc gaggctcacg ccgcgggcgg
     3721 ccgcaccgca ttcgtcctcg atcgccacgg cgacaagctc cgcgagttgc tcctgcgtga
     3781 caacggcgtc gagcgcacgg tcgcctatct gaagaagaag atgcagctcg gctacgacta
     3841 catcgtgatc gacgagatca cggcgtcggc ggactttcgc gacggcacga ctctaaatcg
     3901 ccgtctccgc aagctgctcc tccgcatgcc ggcgcgcacg atcattccgt acatctcgat
     3961 cgacctcacg cagcagacgt acggcttcgc ggcgatgcaa ggccgcaagc tgttgctccg
     4021 cgcgttcaag ctgcgcgcac ggtcgatcgc gctcgaggtc tacctccaca cggctcaggt
     4081 tcgcgccggc gcagcgcccg caacgtttcg ccgcgccgcc gatcggctcg cactcgcggt
     4141 gcgcggcctc tcgaagaccg ccggcatcaa ccggcgcgcg atcaccgtga tcggcacgtc
     4201 catgcattcg tcctacccgc agtatcgcta cctcgaccaa gcgagctacg acctcgcgtc
     4261 gctgtcacgg caggtgaacg cgctccgtca cggcagcaag cgactgcgcc aacagaaggg
     4321 cgtgggctgg tacttcgtga acaagtccga catggcaccg ccgagcacgt acagctatga
     4381 cgcgctcatt cgccggatgc gtttgctcgg ccttcgtttc aaatagggca cggtcgttgc
     4441 tgagtcctcg gcaatgcacc gaggtcgtgt tgtaactgct ctatctttgg tcggcgcgag
     4501 cgccctggcc ggacttgtcg gccgctcgat cgtgcggaag cgtcgcgctg agcggcaaac
     4561 gatcgtcagc gaggtgtttc cacccgaggt cgtggcgaaa atgcacgacg agaaggtgca
     4621 gcgcattgcg gcgcagcttc gctcccacga cggcagcaag ccggtatcgc tgcgcaagaa
     4681 ggcgccgccc caccaagtgc cgaaaggcgg cgatctgcgc cgcaaggacg acaagatcga
     4741 cgtgtccgac ctgacctcga tcatctcggt cgatccgatc tcgcgcaccg ccgttgccga
     4801 atccggcgtc atgttctgcg acctcgtcga ggcgacgctg cgccacggcc tcgtgccgat
     4861 cgtggtcccc gagctgaaga ccatcacgat cggcggcgcc gtctcgggct gctcgatcga
     4921 atcgatgtcg tatcgctacg gcggcttcca cgacacgtgc ctcgagtacg aagtcgtcac
     4981 ggcgcaaggc gaggtcctca cggtcacgcc gagcaacgag cacgcgctcg tgttccagat
     5041 gatccatggc tcgttcggca cgctcggcgt cctgacgaag ctgacgttca agctcgtccc
     5101 cgccaagccg ttcgtgcgcg tcgattacga gcgctatcac aacatcaagg actaccaggc
     5161 cgcgatccag cgccactaca aggcgcagga cgtcgacttc atggacggca tcatccaccg
     5221 cccgaatctg atggtcctgt cggtcggcaa gttcgtcgac aaggcgccct acacgaacgc
     5281 gtacgactgg acgaaggtct actacgcgtc gacggcgcgc ctcgatcacg actacatgaa
     5341 gacgcccgac tacttcttcc gctacgaccg cggcgtgaca aacgtgcatc cgaaatcgtt
     5401 catcggccgc ctcttcctcg gcaagatcct cgattcaggc acgacgctgc ggctcgccga
     5461 caagctgaac ttcatgttca agacggatcg cccgacggtg atcctcgacg tattcatccc
     5521 gttctcggcg gcgcccgcgt tcatggagtg gttcgaaaaa gaggtcggtc acttcccgtt
     5581 gtggtgcgtg ccctacaagc gcgtgcacga ctacgaatgg ctcgacaact cgttctacgc
     5641 gaacatgaag gacgagctgt tcctcgatct cgcgatctac ggaatggagc agcccgaggg
     5701 caagaacatc caccgcatga tcgaggagaa gctgcgcgag ctcggcggcg tgaagacgct
     5761 catctcgcac aactactact cgaaggacga gttctggcag atctggaaca aggacaacta
     5821 cgagaaggtg aaagcggtca ccgacccgaa gaacgtgttc cgcgacctgt atacgaagac
     5881 gtgccgcgcc gcgatgggca ttgccggctg agatacagtt gccccgatgg ggcgcttcct
     5941 cgcggcggta ttcgtactcg gtctgacttc gacggcgttc gcgcagggac cacgcaccgg
     6001 cggtcccgtg ccgggcaccg tgcagggcgc aaaggcgtcg gtcgatctgt cgctgacggg
     6061 caacctcgcg cgcggcatct ccgatcgcga tctcgtgatc gggcgcggca acctgcagta
     6121 ctggacgggc ccgtggggct tcttcctgca gccgtattac ctctacggcg acgtcaagct
     6181 cggcgcgatg ccgcgcgtga agaccgacga cgagcgctat ctccgcgcgt ccgtgttccg
     6241 gacactcgtg aagccgatgt tcacgtatgg cgtggtcgcg ctcgaccact cactgcggcg
     6301 ccgcatcgat cgtcgcgcgc tcgcgggcgg tggcgtcggc gtgacgctga cgaaccaggc
     6361 ggatatcacg ttcgtgacgt cggtcggcct gctctacgag cacggcgagt atggcgaagg
     6421 caaagtccag gaccagatct ttcctgcgaa gaaccgcaac gtggtgcgca cgagcctgcg
     6481 gctgtacggc atctacaagc tgccacacgt caatctgatc cacgacgtct acttcattcc
     6541 caacgtgcgt gacctccacg atctgcgcgc gatgttctcc ggcgtcctcg aggtgccggt
     6601 ggcgaagggc ttcgcaggac gcgtctcggt cgatgccacg cacgagggcg tcatcgtcga
     6661 gggcacgcag gaagacgaca tcgcgatcac gtttggcgtg tcgttcaaga acgactggac
     6721 gttcgacccg ccgcctccgc cgcccgcgcc tacgccgggt gcgccggcga tgccctagcc
     6781 acgatctcga gctcttgctt gagcttgacg atgctctgcg cgggatacac gcccggccgc
     6841 agctgcacgc ctggatagat ctgcgtccct cgcccgacga cgcagccggg atgcagcacc
     6901 gagttgcagc ccgtgagcac gccatcgccg aggatcgcgc cgagcttgtt gcgctgcgtg
     6961 ttcgttcccg cgatctgaat gctggccgcg tcgtggcggt agttcgagag gaccgtcccg
     7021 gcagcgaggt tcacgttcgc accgaggatc gagtcgccga cataattgag atgcgatgcc
     7081 ttcgcgccgg ggaacaggat gccgcgcttg atctcggtgt tcgtgccaac gacgcagtag
     7141 tcgccgatcc acgagccgcc gcgaatgtac gcgcccggcc gtacgtcgac gtgcttgccg
     7201 atgaagacgg ggccttcgat gacagcgctc ggatgaacgc gagtcccttc tccgatgagg
     7261 tctagtcctg cgaggatcgt atcgagcgca tcaccgagca gcgtccaggg cgtctctgga
     7321 tcgtaatgag agacgaagac cgacggcaga ttcgcgaaga gcggtggcag caccgccgaa
     7381 gactacgtca gtcgatgccg cgaattgcgg gctcgtcgtc gacatcgatc cagcggcgcg
     7441 ccgcgacgca gcgctcatcc atcacctggc gctcccgccc cgtgtccttt tttgacacag
     7501 ccttcatctc acgggtggtt cggtgttggg ggtcggcgct ctcgttcatg gggagttggg
     7561 tctgcaatgg gtgtgcttgc ctactgaccg cggttccgag cgataacgcg ctgcgattcg
     7621 tgcgtacacg cgcacggggc ttgggcgcgc gagaacgcga ccgtgatact gcacggcgat
     7681 gaagaagctc ctcgctcctc tcgttctgtc ggcggttctc tcgggttgct acggctcgta
     7741 cagcgcgagc catgcgcttc acaaagcgaa cggccacatc ggcagcccga tcgcgaactc
     7801 cgccgttcat ctcgtgctgt ggatcatccc ggtctacccg atcgtgtatt tcggcgactt
     7861 cttgatcttc aacaacatcg agttcctcac gaacaagccg gtcttcaact gaccgcgcgc
     7921 cggcgcgcag gcgtggcaat cagctgcgta ctgcttgcac taagtgacga gaacacatct
     7981 cgcgacgcgg tgcgacgttt gcaaaccggc cgcggcatga tgaagatcct atcgcgcgtt
     8041 ctcgttacgg cacttctgac ggtgtctttc gcggcttccg ctgatgacac cacgacgaaa
     8101 acgcaaccca gtgatgcgcc gaagacggcg gagacgcccg cgaagccggc gaagcccgcg
     8161 aagctgaagc aggccgagct ccaggtgatg gcgcactacc acgccgtcaa caagatggag
     8221 atcgatctcg gcaacgccgc cgcgaagaag gcgatgacgg atgcggtgaa agagtacggc
     8281 aagatgctcg tcaaggacca cggcgagtct gacaaggcgc tcctcgcgct cgcgaagaag
     8341 accggtcagg tcattccggc cgagaagccg gccgacgaca tcgagaagaa ggagaaggcg
     8401 gacgacaagg cgcaggctgc gaagctgaag acgctcaagg gcgctgactt cgagcgcgag
     8461 tacctccgca tgatggtgca gggtcacgag aaggagctcg gcaagatcga cgcgaacatc
     8521 gcgaaggtcg agaacgcgga gctcgcggat gcattgaagg cgaagaagcc ggtgctgcag
     8581 catcacgcgg actccgcgag ggagctgcag aagaacgagc cgacggcgag ccgctaacgc
     8641 tccgcagttg cgcacgttcg tacgcaacgc tggggaaact gcatgggact agtccccgca
     8701 gtgcaaacgg ccgtctgcgc gcaatcgtga ggtcgcgtac ggcgatcaca atcaaacgct
     8761 gcacgcgtcg cgcaacgcgt tgcacggagt gatcaagact cgcattttgc ggcagcgggc
     8821 atattggctg cagaacgccg cggccgtgaa accgctagcc atctgtctgt tcgtcacggc
     8881 aaccgctgcg tgtactgcgg acgtcgagat catcactgaa gatcaagaag ccacgcccgg
     8941 cgcgagcatc acgaccgagc gcagcctctc cggttgcaac ggccacgcgg cgacctcgat
     9001 ccccgcagat ggccgctacg tgatcacgac gttcggcggt cccggcgatc accaaccgat
     9061 gtcgtgcggt ggctacgcgg atggcacggg ttggtacgcg gcatcgcgcc agcgctacgg
     9121 ctgcggctct cacatcaagg tgcaagcgaa cggcaagtgc gtcgtgctca agacggacga
     9181 ctacggccct gacgtgtgcg tcgagacagc ggcacacatg ccgatcatcg acgtcacgcc
     9241 gatggcatcg aaggcgttgt tcggcgcgtc gggtgccggc tggagtgatc atctcgttgt
     9301 cacggtcgaa gaagtcgcga cgtccgttcc gctcggtcca tgtacggcaa cgcctcccgg
     9361 tggcggcggc ggcggtacga tgaatccgcc ggctggctcg gcctcgtgcg catccgcgac
     9421 gctcgatcgc gatgtcgaag acggcgtctg cgtgcaatcg gcgacggact cgctctggta
     9481 cgcctgtaac aacggcacgt gggcagctcg cagctcctcg gccggctgcg ccgaagccta
     9541 cgggttctgc aactcggcca cgctcggcaa gaatgtcccg gctcggacct gtgtgcaggc
     9601 cgcgtcgaat agcgtctggt atcagtgcaa tggccagggt tgggtcacgc ccgtcagcac
     9661 gtccgcgaag agcggcccgc tcggggactg cgcgacctgg aacccgctct aatccgagcg
     9721 ctgttacagt tttccgacga cgataaaccc gagcgcgggg tcccctctcc tcgtctatga
     9781 tgcgcggcat gttccgcctc gacgagcaga gtcaaatggt cgccacgatg gtgcgcgggt
     9841 ggtgtacgtc ccaactcgca ccgaagattc cagcgctcga gcacggcacg gagatgccgt
     9901 tcgacctgat gaagaagatg gcgaaggcct tcggcttcga cgcgatgctg ggcgccagcg
     9961 tccaaaagcg aatcgcaaag ctgcgcgcag gagaagacac gactgcatcc gcagagaaga
    10021 cgcttgccgc gggcgtcggc aatccgatga tgatgcacgt cgtcgtcaag gagctgtcgc
    10081 gcgtgtcgcc cggcttcgca atgggtttcg gcgtgtcgat gggcctcgtc ggcggcgcga
    10141 tcgtcgcgaa aggcacagcc gatcagatcg agaagtgggg cttgcccgtc gcgacggtgc
    10201 agcaaatcgg atcgtggtgc ctcaccgagc caaacgccgg cagcgacgcc ttcggctcga
    10261 tgcggacgac cgcgcgcaaa gaaggcagtg actacattct caaaggctcg aagacgttca
    10321 tcacgaacgg gcccggcgcc gacatcttcc tcgtctacgc gaagaccgaa gacggcggcg
    10381 tcgcgacgtt catcgtcgag cgcaacatgc ctggcgtctc cgtcggcccg ccgttcaaga
    10441 aaatgggcat gcgcgactca ccgacgagcg aagtcttctt cgacgacgtt cgcctcggcg
    10501 tcgagcatct cctcggcggc aaagagcgcg gcaaagccgg tcgcggcgac acgaaagact
    10561 cgctcggcag cgagcgctcc ggcgtgcccg caatggcctg gggcatcatc gagcgctgct
    10621 acgacgcgag cctcgcgtac gtgcgcgagc gcgagcagtt cggtcgccgc atcggcgagt
    10681 tccaagcggt gcagctcaag atcgccgact tgttcctcaa gctgcgcacc gtcgagaacg
    10741 tcgtgttccg gacttcgtgg atgcagcaaa acggcgtgcg tgacatgccg ttcgtcaacg
    10801 cgtcgaaggc gatgtgcgcg cagatggcgg tcgatgccgc gctcaccgcg gtccagatcc
    10861 acggcggcta cggctacatg gaagagctcg ggatcgagaa gctcgcgcgc gatgcgaagc
    10921 tgctcgagct cggtgctggc acgacggata tcaacttgct ggcgtgtgcc cgcgagctga
    10981 tcggcgtgca tggcaacgag taggctctcg ctgctggtcc tcacgctcgc cgcgtgcacg
    11041 ccgaacgtgc cggagaagaa gccgccgaag ggcgcgctga tcgagaccct cgccgcgggt
    11101 agcaatgcgg tcgtggtcga gacgatcgag cctgcggcga gcgcgagccc ggaggcactg
    11161 cggcagtacc tactctcgaa ggcgccgatc gatcaccgcg cagccctcgc cgacggcttc
    11221 acggccacat tcggcggcga gacgcacgtc ctgcgcgagc ggcacaaccg ctggtatcgc
    11281 tgccacgcgc cggtgacaga cgatgcgatg cgcgacgacg tgatcgcagt ctgcaaatcg
    11341 tacaagctcc ctgcgcaatg atcctcgcgc ctctcgcgat cgccgccggc ctcgatgtcg
    11401 gtcgcgtcgt cgataacggc cagatccacg agaaccgcgc gctcgtcgac gcaacaggca
    11461 tcggctgggt gcgcatcaac ctccgcctcg atacgaagag cgcgatcgag gacgaattcc
    11521 cgatctacga ccacgtcatc gacgactacc tcgcgcgcgg gattagtgtc tacatcctga
    11581 tcaacgacga ggcggtctcg tcaacgctcg cccacggcag tgacgcgtgg atcgagcaag
    11641 cgttcgtgcc ggcggcggtg aagatcgtcg atcacttcaa gaaccgcgtc cgcgtctacg
    11701 agatcatcaa cgagccgaac gacttcgcag gcggctcctc cgcccggttc tcgccccgcc
    11761 agttcgccaa gatcctccag gacacgtacc tcgccgtaaa gcacgacggg ggtcatgacg
    11821 ccgatgcgtg ctggcaggtc gctctcaact ccggcccgct gttctcgttc gatgacacgc
    11881 cggccgacac atacctcgat caaacgtacg cgatcggccg ctcgcagctc gcgtgggact
    11941 acacgcacca aaccacgggt cgctatccgc tcgacggcat cggctatcac atgtacgtcg
    12001 cgcaaggcct cgactcatcg aatgccgacg tccgcacgca gatgctcgcg aacctgtccg
    12061 cgatcaatgc agtcgtcacc gcccgcgacg acgcgaccaa gccgctatgg gtcagcgaat
    12121 acggctggga agcatccgtc gtcggcaacg acgtccaagc acagcgcctc accgcgggct
    12181 tcagcgcgat ggccgaagcc ggcaccgtcg cgacggcgtt ctacttcaac ttccaagact
    12241 tccccggcgc gacgtacggc atctacgatg acgcgctcgc cgttcgcccc tccgctgccg
    12301 cgctctcgca gatcgcaccg ctgccgctgc gcgcctcgat cgagcaagtg atcgtcccgc
    12361 cgacgatcgc tcctggcgcc gatgccgaca tcgtcgtcac gctgcgcaac cgcggaaccg
    12421 aaacgtggac gccgcaagcc ggctaccgcc tcgccgccgc ggccggctgc ccggacgcat
    12481 ccgccgccaa cgagatcgcg tggaatcccg cagccaccga cggctacgca acatctccga
    12541 tcgacgcacg catctacctg cagcactcgg tcgccgccgg agacctcgtc gaaattcacg
    12601 tacccattca cgcgcccgag actaccggct cgtacacgtt cgccgcgcgc atggtgcaag
    12661 aaggcgtcag tctgttcggc ccgacgattc gcgcgacgat cacggtcgcg aacgcccccg
    12721 tcgacggtga cgacacttcg acaaccccgc cgccaacttc tccaggcgga tgctgctctt
    12781 cgacgaccag caattcctcg acgacgctcc tttcgctcgt cgtctccgtg ggcgtcttgc
    12841 tcctccgaaa acgtcgccgt ggcacatccg ctgcagcctc gcgagtccgg aggttctcct
    12901 atgctgtcga ctcgctttct cctcgccgcc tctctcgccg tgctcgcacc cgcgaccgcg
    12961 gcatgcaccg atagctcgac cacgggtgtg gtcgtcgacg atcccgtcgg agacacgatc
    13021 gacgaaggcc gcgcgaccgg caccaccatc gccgcccaag ccttcggcga gctctccgcc
    13081 gacgactacc agacgatgat cggcaaaaca ggcgagctcc tcgcgacgct ccacgacggc
    13141 gaaatccaac aagcgtcgtt cgcgatccaa gtcgtccagg acgacgacgt cttcgattac
    13201 gccaacaaca tgatcatcga gcacgaagac ggcctcgccc tgctcgacga cacgatgcgc
    13261 ttctacggcg ccccgtacat cccgagccaa gcccaagctc agctcatggc cgacgcgcaa
    13321 gccgcgctcg ccgacctccg cgcaacaccg ccgcaagaca tcgacttccg ctacatggaa
    13381 gtccaagtcc gaatgcacgc atccgcacaa gtcatcctcg atcagctctc gcagctcgtc
    13441 ggccccggcg cgatgaatga ctacatcatc aacgctcgcg gaatggtcga cgagcacttc
    13501 gaccgagcga ccagcatcct cgagacgtac tactgagtgc cgcccgacgc ggacacagcc
    13561 gccttcgctt tcgcgtggca gctcgacttc gcagcaaagc catatatcgc cgacacgaag
    13621 aacacgccgc cgacacccgc aatgatggcg gcgtttctga gcgccgcttc gccgcattcc
    13681 acgccgccgg cgccctcgca tccgccaatc gccttggcca cctgcgaaag agctaggaag
    13741 ccggcaagcg tcatcgcacc caggtccacg cccgcgacca cagccgattc gcagtacggc
    13801 atcttcgtcc ccgcatcgac gcgcgggctc ggcctcatga tcgcgaacga gcagctgcaa
    13861 agcacacaac aggcgagcgc gatcctccac atccgccgat gatgcacgtc tgctctcgaa
    13921 cgcgcgatgc cccgaacggg taagcgcctt cgctgccacc ccgagagccg gaccaagaag
    13981 tgacgtacgc gccccgctga caagcccttg cgcggctgca cgcgagccgg gcgacgacct
    14041 cgccacgatc gccagcaacc ggcgacctac tccgcagcat cgccgcgccg cgcccagctc
    14101 gaagtgcaat cgcgaaacaa gaaactcacg agccgcgcct gggccggcgg cgatgtcgcg
    14161 gcgcgcgccc ctggccgt
//
LOCUS       DATLBZ010000037         5682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1519930, whole genome shotgun sequence.
ACCESSION   DATLBZ010000037 DATLBZ010000000
VERSION     DATLBZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519930"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..925
                     /locus_tag="VMZ53_01885"
     CDS             272..925
                     /locus_tag="VMZ53_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HUS27219.1"
                     /translation="MGKADRRREEKARALAKVEERKRVALRVLQDCDEAGARAIAKLA
                     VSGRGPSCVAGCTHCCRLEIPMSRAEGEVIVDWLSANRSPEQVEAVRERLRAWLAWYR
                     TELPRLVASGVFRVDAFARHSPACALLVDGQCSAYPVRPVTCRNHYVSSPPEVCDPAT
                     STREPDSLLEIPRATQPYVVELRRVVERQGGDYMASIHLIGEWLAHLLEVEREPWRG"
     gene            938..1750
                     /locus_tag="VMZ53_01890"
     CDS             938..1750
                     /locus_tag="VMZ53_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HUS27220.1"
                     /translation="MKVATFNINGINTRLANLVEWLEREEPDVVCLQELKANERQFPA
                     KALREVGYSAIWRGEPLWNGVAILSRGSAPIEIRRALPGQEHDEHARYLEAAVKGVIV
                     AGLYLPNGNPIRGPKFKYKLEWFEALIAHAKSLFEHEAPVMLIGDFNVVPTDNDIYNT
                     RSWKRDALLQPESRACWQRLLAQGWVDSLRAVHGDARIYTFWDYLREAWKRDAGLRID
                     HLLLDKKLAPRLVDAGVDRWVRDRPKASDHAPTWIVLDDKAAKPRRRRKGLS"
     gene            1755..3431
                     /locus_tag="VMZ53_01895"
     CDS             1755..3431
                     /locus_tag="VMZ53_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUS27221.1"
                     /translation="MTPDAAFRDARDVLLRHATDLAAARAAFRWPALEYFNWVDHWFQ
                     PYARGNRRDALRVVTDAGTTALTFDELELRSRSVARYLRDAGVERGDRMLLMLTNVAP
                     LWETVLAAIRLGVVLIPATAQLTPADLDDRIERGGVRHIVTDADGAAKLRDPERLRVR
                     LSTVDVDKFARFDDARAAEPKIDEVRTRADEALLLYFTSGTTAKPKLVLHTHASYPIG
                     HLSTMYWVGLRENDVHQNISSPGWAKHAWSSLFAPWNAGATIVAHDQPRFSARRALEV
                     LREQNVSTLCAPPTVWRMMILEDLGPRPPALREIVGAGEPLNPEVIDRVERAWGITMR
                     DGFGQTETTAVIGNAPGQQVKPGSMGTPLPGYDATIVDAAGNPAAEGEVAIRIAPSRP
                     LGLMQGYLDDPERTAAVMAGGLYRTGDEARIDDEGYVHFVGRGDDVFKSSDYRISPFE
                     LESVLVEHELVAEAAIVPSPDALRLAVPKAFVVLRPGTPETEATARSIFAFVRERLAP
                     YKRIRRLEFAALPKTISGKIRRVELRNMERDRRAREERGPLEFWSDELER"
     gene            3533..4987
                     /locus_tag="VMZ53_01900"
     CDS             3533..4987
                     /locus_tag="VMZ53_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HUS27222.1"
                     /translation="MTEHAVVIAGAGPTGLMLAGELALAGVDVAIVERRQSQVLEGQR
                     AGGLHARTIEVLDQRGIAERFLAEGKTAQVTGYALNMLSIEDFPTRHPYGLGLWQNHI
                     ERILAGWVEEQGVQFYRAREVTGFAQDETGVSVALGDGTSLRAQYLVGCDGGRSVVRK
                     AAGIEFPGWDPTVSCLIGEVDLAEEPAVGIRTDARGTHGLSKLEQGTVRVLITEHTVE
                     TGDPTLADFSAGLKSVYGTDFGLRGATWLSRFTDTTRQAAKYRERRVLLAGDAAHIHP
                     PHGGQGLNIGVQDAVNLGWKLAQVIAGTSPDSLLDTYHAERHPIGARVLKHTMAQVAL
                     RRIVDDRSTALNDVFADILKMDEPRKRFAGMMSGLDVHYELGEGHPLVGRRMPDLDLV
                     VDGGTRRVYSYLHRAWPLLLDLGDVASVDVSPWASRVQVVRARVEGEWQLPVLGVVPA
                     PRGVLVRPDGYVAWVGEVSDPRLLDALTTWFGPR"
     gene            4999..5457
                     /locus_tag="VMZ53_01905"
     CDS             4999..5457
                     /locus_tag="VMZ53_01905"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HUS27223.1"
                     /translation="MARVAGKSKMKKTSTTANKTKAKPAKAKPASDGASKLIDGRIAE
                     LADWRGPMLSKLRKLIRDADPNVVEEWKWEVPVWSHDGIICTGESYKTRVKLTFAKGA
                     QLKDPKKLFNSSLEGNVRRAIDFAEGAKIDEAAFKALIREAVALNESKAS"
     gene            5488..>5682
                     /locus_tag="VMZ53_01910"
     CDS             5488..>5682
                     /locus_tag="VMZ53_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27224.1"
                     /translation="MRALEQHLGRIRNAGSGLLHYSMWDDHGREHATEEYAREMEALR
                     AESMSAREAGLAGLRALVPVV"
BASE COUNT         1005 a         1769 c         1988 g          920 t
ORIGIN      
        1 cgctgtcggt gacaccggtg ccggcgtccg gaggtgggcc cgacgccgct ggtccacagg
       61 ccacgacaaa cgcgatgacg gaaggaagac ccctcgttgg cttcacgcac gtagcaaagc
      121 gacatccgcg cgatcgagtc aacgacaacg atccgtctcc gcgtgatttc gatcgcgcag
      181 atcgccgatc gcgagcaaca cgtgcacgtc attgcgcgat ccgtgcactc gcgagccata
      241 gatcctcgct cacgcagtaa gctcacaagc gatgggcaag gccgatcgtc gacgcgaaga
      301 gaaggctcgc gcgctcgcga aggtcgagga acgcaaacgc gtcgcgcttc gcgtgcttca
      361 ggattgcgac gaagcgggcg cgcgagcgat cgcgaagctc gcggtgtcag ggcgcggtcc
      421 gtcgtgtgtc gcgggctgca cgcattgctg ccggctcgag attccgatgt cgcgcgcaga
      481 gggtgaggtc atcgtcgatt ggctctcggc gaaccgctcg ccagaacaag tcgaggccgt
      541 gcgcgagcgg ctgcgcgctt ggctcgcgtg gtatcgcacg gagctgccgc gcctcgtcgc
      601 atcgggagtc ttccgcgtcg acgcgtttgc gcgccactcg ccggcgtgcg cgttgctcgt
      661 cgacggtcaa tgcagcgcat atccggttcg tccggtgacg tgccgcaatc actacgtgtc
      721 gtcgccgccg gaggtctgcg atcccgccac gtcgacgcgc gagcccgatt cgctgctcga
      781 gatcccgcgc gccacgcagc cgtacgtggt ggagctccgg cgcgtcgtcg aacgccaggg
      841 cggggactac atggcctcga ttcatctgat cggcgaatgg cttgcgcatc tgctcgaggt
      901 cgagcgcgag ccgtggcgcg gatagcgcag ttgcaccgtg aaagttgcga cgttcaacat
      961 caacggcatc aacacgcgcc tcgcgaacct cgtcgagtgg ctcgagcgcg aggagcccga
     1021 tgtcgtgtgc ttgcaagagc tcaaggccaa cgagcggcag ttccctgcga aggcacttcg
     1081 cgaggttggc tactccgcga tctggcgcgg cgagccgctg tggaatggcg tcgcgatcct
     1141 gtcgagagga agcgcgccga tcgagatccg gcgagcgttg ccggggcaag agcatgacga
     1201 gcatgcgcgc tatctagagg cggccgtgaa gggcgtgatc gtcgctgggt tgtacctgcc
     1261 caatggcaat cccattcgcg gcccgaagtt caaatacaag ctcgagtggt tcgaggcgtt
     1321 gatcgcgcac gcgaagagcc tgttcgagca cgaagcgccc gtgatgctga tcggggactt
     1381 caatgtcgtg ccgacggaca acgacatcta caacacgcgg tcatggaagc gcgacgcgct
     1441 gctccagccg gagagtcgcg cgtgttggca gcgcttgctc gcgcagggct gggtcgattc
     1501 gctgagagcc gtccatggtg acgcgcgcat ctatacgttc tgggactacc tgcgcgaggc
     1561 ctggaaacgc gacgccggcc tgcgcatcga tcacttgctc ctcgacaaga agctcgctcc
     1621 gcggctcgtg gatgccggcg tcgatcgatg ggtgcgcgat cggccgaagg cgagtgacca
     1681 cgcgccgacg tggattgtcc tcgatgacaa ggcggcgaag ccacgccgcc gccgaaaggg
     1741 tctatcgtga ggagatgacg ccagacgcgg ccttccgtga cgcacgcgat gtacttcttc
     1801 gccacgccac ggacctcgcc gcagcgcgag cagcgtttcg ctggcctgct ctggagtact
     1861 tcaactgggt cgatcactgg tttcagccgt acgcgcgcgg caatcgtcga gatgcgctgc
     1921 gggtcgtcac cgacgctggg acgacggccc tcacgttcga cgagctcgag ctgcgatcgc
     1981 gatcggttgc tcgctatctg cgcgatgccg gcgtggagcg cggcgaccgc atgctcttga
     2041 tgctcacgaa cgtcgcgccg ctgtgggaga ccgtgctggc tgcgatccgg ctcggcgtgg
     2101 tgctcattcc cgcgacggcg cagctcacgc ccgcggactt ggatgatcgg atcgagcgcg
     2161 gcggtgtgcg ccacatcgtg accgatgccg acggcgcagc gaagcttcgc gaccccgagc
     2221 ggctgcgcgt gcgcctgtcg accgtcgatg tcgacaagtt tgcgcgcttc gacgacgcgc
     2281 gagcagcgga gccgaagatc gacgaggtgc gaacgcgcgc cgatgaggcg ctgctgctgt
     2341 acttcacgtc ggggacaacg gcgaagccga agctcgtgct gcatacgcac gcgagctatc
     2401 cgatcgggca tctgtcgacg atgtattggg tcgggttacg cgagaacgat gtccaccaga
     2461 acatcagctc gccaggctgg gcaaagcacg cgtggtcgag cttgttcgca ccgtggaacg
     2521 caggcgcaac gatcgtcgcc catgatcaac cgcggttctc cgcgcgccgt gcgctcgagg
     2581 tgctgcgcga gcagaacgtg tcgacgctgt gtgcaccgcc cacggtgtgg cgaatgatga
     2641 tcctcgaaga tctgggcccg cggccgcctg cattgcggga gatcgtcggc gccggcgagc
     2701 cgctcaatcc agaagtgatc gatcgcgtgg agcgtgcgtg gggaatcacg atgcgcgacg
     2761 gctttgggca aaccgagacg accgccgtga tcggaaacgc gccggggcag caggtgaagc
     2821 ccggctcgat gggaacgcca ctgcctgggt acgacgcgac gatcgtcgat gcggccggaa
     2881 atcctgcagc ggagggcgag gtcgcgattc ggatcgcgcc gtcacggccg cttggtctca
     2941 tgcagggcta tctcgacgat ccagagcgca cggccgcggt catggcgggc ggtctctatc
     3001 ggacgggcga cgaggcgcgg atcgacgacg agggctacgt ccacttcgtc ggacgcggcg
     3061 acgacgtctt caagagcagc gactaccgaa tctcgccgtt cgagctggag tctgtgctcg
     3121 tcgagcacga gctcgtggcg gaagccgcga tcgtgccaag ccccgacgcg ttgaggctcg
     3181 cagtaccaaa agcgttcgtc gtgctgcgtc caggcacgcc ggagaccgag gcgaccgcgc
     3241 gatcgatctt tgcgttcgtc cgcgaacggc tcgcaccgta caagcggatc cgccgtctcg
     3301 agtttgccgc gctgcctaag acgatctccg gaaagatcag gcgcgtggag cttcgcaaca
     3361 tggagcgcga ccgccgggca cgtgaagagc gcgggccgct cgagttctgg agtgacgagc
     3421 tcgagcgcta accacaggcc gcgctgactc gctgatcgcc cagcatacac tctcgcattc
     3481 gggccgcact tacaagctag gaatctcgcg cgcgttcagc gatcacgaac gcatgaccga
     3541 acacgctgtc gtgatcgccg gagcggggcc caccggactg atgctcgcgg gtgagctcgc
     3601 attagcgggc gtcgatgtcg cgatcgtcga gcggcgacag tctcaggtgc tcgaggggca
     3661 gcgcgccggt ggcctgcacg cacgcacgat agaagtgctc gaccagcgag gcattgccga
     3721 gcggtttctc gcggagggaa agaccgcgca ggtcacgggc tacgcgctca acatgctgag
     3781 catcgaagat ttcccgacgc ggcacccata tggactcggc ctctggcaga accacatcga
     3841 gcgaattctc gccggctggg tcgaggagca aggcgtgcag ttctatcgcg cgcgcgaggt
     3901 gacgggattc gcgcaagacg agactggtgt cagtgtggcg ctcggcgacg ggacgtcctt
     3961 gcgcgcgcag tatctcgtgg gctgcgacgg cgggcggagc gtcgtccgca aggcggcggg
     4021 catcgaattc cccggatggg atccgacggt gagctgcctc atcggcgaag tcgacttggc
     4081 ggaggaaccc gccgtcggca ttcgtaccga cgcgcgcggc acgcacggcc tgagcaagct
     4141 cgagcagggc accgttcgcg tgctgatcac ggagcacacc gtcgagacgg gcgatccgac
     4201 gcttgccgat ttcagcgcgg ggctcaaatc ggtgtacggc accgacttcg ggttgcgcgg
     4261 cgcgacgtgg ctcagccgct tcacggatac gacgcggcag gcggcgaagt atcgcgaacg
     4321 tcgcgtgctg ctcgccggcg acgccgcgca catccatccg ccgcatggcg ggcaggggct
     4381 caacatcggc gtgcaggacg cggtgaacct cggctggaag ctcgcgcagg tcatcgccgg
     4441 cacttcgccc gacagcttgc tggacacgta tcacgccgag cgtcatccaa tcggcgcgcg
     4501 tgtgttgaag cacacgatgg cgcaggtcgc gctccgtcgc atcgtcgacg atcgaagtac
     4561 cgcgctgaac gacgtcttcg ccgacatctt gaagatggac gaaccgcgca agcgcttcgc
     4621 tgggatgatg tcgggtctgg atgtgcacta cgagctcggc gaaggtcatc cgctcgtcgg
     4681 gcggcgcatg cccgacctcg atctcgtcgt cgatggtgga acgcggcgcg tctacagcta
     4741 tctgcatcgg gcatggccac tgctgctgga tctcggtgac gtggcgagcg tcgatgtatc
     4801 gccatgggcg agtcgcgtgc aggtggttcg cgcgcgcgtc gagggtgaat ggcagttgcc
     4861 cgtgctcggc gtcgtgcctg cgccgcgtgg cgtgctcgtg cgaccagacg gctacgtcgc
     4921 ttgggtcggc gaggtcagcg atccgcgact gctcgatgcg ctgacgacgt ggttcggacc
     4981 gcggtaatgt tcgtcggcat ggcgcgcgta gcgggcaaga gcaagatgaa gaagacctcg
     5041 acgactgcga acaagaccaa ggcgaagccc gcgaaggcga agccagcgag cgacggcgcg
     5101 tcgaagctca tcgacggacg catcgcggag ctcgccgatt ggcgcgggcc gatgctgagc
     5161 aagctgcgca agctgattcg cgacgccgat ccgaacgtgg tcgaggaatg gaagtgggag
     5221 gtgcccgtgt ggtcgcacga cggcatcatc tgcacgggcg agagctacaa aacgcgcgtc
     5281 aagctgacgt tcgcgaaggg cgcgcagctg aaggatccca agaagctgtt caactccagc
     5341 ctcgagggca acgtccgccg cgcgatcgat ttcgccgagg gcgccaagat cgacgaggcc
     5401 gcgttcaagg ccctcatccg cgaggccgtc gcgctcaacg agtcgaaggc gagttagcga
     5461 gtggcgggta gcgcggcaac gcctcgcatg cgcgcattgg agcagcatct cggccgcatc
     5521 cgcaacgcgg gcagtggact gctccactac agcatgtggg atgaccacgg ccgcgagcac
     5581 gcgacagaag agtacgcacg ggagatggaa gcgctgcgcg ccgaatcgat gagcgcgcgc
     5641 gaagcgggcc tcgcgggact gagggcgctc gtgccggtcg tg
//
LOCUS       DATLBZ010000038         5886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3757527, whole genome shotgun sequence.
ACCESSION   DATLBZ010000038 DATLBZ010000000
VERSION     DATLBZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3757527"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VMZ53_01915"
     CDS             <1..209
                     /locus_tag="VMZ53_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27225.1"
                     /translation="HALRRQYQLQRLMKTMGQGGTAEAEHLDTLALEWLGVGPTEEAT
                     YMPLIDRFKECRKRALATPRRGRN"
     gene            complement(251..1729)
                     /locus_tag="VMZ53_01920"
     CDS             complement(251..1729)
                     /locus_tag="VMZ53_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27226.1"
                     /translation="MSKTIALVIAGAVLCVAAPTARAADRTFPAGSLIIPMDLSYQSH
                     GMFQAYGLIYQLTKQGVHVHWVIDPNKTYHQAPCNTANDLCAWDCGVEGASAKCAYPT
                     ASPDFTATTNVIWDDATSARPGTSLGTHKYRGGPFVVDAADHDHALQIIDVWNDKTKW
                     AANPWATRTIFHVVSVHEATASFTGNSAREMIAAPTIAVFADGTEDIATGYLRAAGIP
                     QSNGKEFPSAKCGTCGPGTANPDMLTEEAIMGDMGTCDAPNNDHHNGALFKADGTPAY
                     CQIMSMHWNVGDRETVKCGGGNCPATQQLCAAGTKITYNGHEVVAEVRAFLAYPTHFF
                     AECQAVNAYENTVPNPNWPFLDDAGRDGHFLTTTGTPPACTGTCTNGDYQCTQSDLYC
                     GTGLSCCLPKAGTWKNLPGYEVAQPAASNLTKVLRPDVPYNQLDGMWGVTGGSEPAYN
                     LSSYYGTTYKNNRQVTLLTGPNGPEPIILKTGLTLPVRNPTQ"
     gene            complement(1825..2904)
                     /locus_tag="VMZ53_01925"
     CDS             complement(1825..2904)
                     /locus_tag="VMZ53_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS27227.1"
                     /translation="MTTKRDPQERSSTDEGLRSERKKTDAELAARSKSLLETTAGAIS
                     DARGKADSVLNQARDREDVQTGASTQVSDARAHEDAIIRAERLGADADTRDEVELQRI
                     ALASLLAFERKDTDLRLNLERKRADQVLASREDFMAMVSHDLRSLLGGIALSADLLKG
                     VERSEQPFVKVTHYAERIQRFSARMTRLVGDLVDVVSIDAGKLSLVRARYDVEQLLRD
                     AHDAFEPAATAEGVELTCTPEGNLGSVELDHQRILQVLTNLVGNALKFTERGGTIIIR
                     AKRLDDAIGFSVSDTGSGIPTELLEKVFDRYFQSEGGDPRGLGLGLYISKSIIDAHGG
                     RIWATSTPGKGTSIFFTVPPLPVAS"
     gene            complement(3028..3762)
                     /locus_tag="VMZ53_01930"
     CDS             complement(3028..3762)
                     /locus_tag="VMZ53_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27228.1"
                     /translation="MRIAFTTRQTSAIAVACVVAVVVALGIWTFDRSEQQQAPSSAAL
                     DVHEPQLELDLQLDFQIEPSSSSVQRESSPPPEQPFYASLFVAGATWDLPCTFGVGIH
                     DELPASMQRCHVDSVEVTADETRARIACWFVKDPDVRDPAPAINTYVMTANGLYINDV
                     RGEPVFTPSPTSKPLPKDWGLHEPSNLEGDNAHAMVRHHGAWCRIDEFQGWDASGGTA
                     ECISRRGLVGSSYSHDMMTQRCGDVP"
     gene            complement(3796..>5886)
                     /locus_tag="VMZ53_01935"
     CDS             complement(3796..>5886)
                     /locus_tag="VMZ53_01935"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS27229.1"
                     /translation="ARVVSSQATMLILDNDREYARYGIDRNALVDILVVGKDGLEQQH
                     RSFVASKDRDGRGGKGHRNQEADGTLGYGRHGFGAGGGGGGWGTIGTGRYGTVGHGSG
                     SGTGSGFGVGSGRGGLRGRVASVPTVSIGQPMAMGSLDKSSVRRYLQRHLNSIRYCYE
                     KELLARPSLHGQIATSFTIAPDGKVTDASATGFDPAVAQCIVDVLRRVDFPASHDETV
                     RVNYPFTFRTAETPADDNAAPDDIQRLVQQSTGAAAPSPAPPPTSAPMPAPPATPTAT
                     QTIASSTAAAPTTTTTTTPTTTTTPTTSAPTSVAPAGTPTTTTPTTTTPTTTTPTTTT
                     PPTTTPTTSTIATPSTAPSTAIDSADDDSRVTLPAPAPVQPRVTVSRPDDTIPRFNSA
                     ASALRGNLATIMRLIAKHDPTALEQAHAWRDSQPTDVLAIVALGEALEAAHETSAAAR
                     VYGSLIDLYPTRADYRRFAGERLERLETAGRPLAVDSYRRAVADRPDQLTGHRLLAYA
                     LVRSNDLAGAFDAILKGVDQKHPDDRYRGADRVLRRDAQMIASAYLAGGGDRATIEKA
                     LAKRRLTAFTGRSTRVLLYWETDANDVDLHIRDARGGHSWYSHLPLESGGELYADVTT
                     GYGPECFEIVGTPTAGPYQVGLHYYRQGPMGYGMGLLQVVRFDGRAFTFEDRPYVIMQ
                     DEAYVSLGTFGARP"
BASE COUNT          925 a         1841 c         1990 g         1130 t
ORIGIN      
        1 accacgcatt acgccgccag taccaactgc agcggttgat gaaaaccatg ggccaaggtg
       61 gcacagcaga agccgagcac ctcgacacct tggcactcga atggcttggc gtaggaccca
      121 ccgaggaggc gacgtacatg ccattgatcg accgcttcaa ggagtgccgc aagcgggcgt
      181 tggccacgcc tcggcgtggc aggaactagc gacgacgacg ctcctcccag atagcgatcg
      241 ctggcgcaga ttattgcgtc ggattgcgta ccggaagtgt cagccccgtc ttcagaatga
      301 tcggctcagg gccattcggt ccggtcagca gcgtgacctg gcggttgttc ttgtacgtgg
      361 tgccgtagta gctgctgagg ttgtaggcgg gctcgctgcc accggtcacg ccccacatgc
      421 cgtcgagctg gttgtaagga acgtcgggcc gtagcacttt cgtcaggttc gacgcagctg
      481 gctgcgcgac ctcgtacccg ggcaggttct tccacgtgcc ggccttcgga agacagcagg
      541 acaggcccgt gccgcagtac agatcgctct gcgtgcattg gtagtcgccg ttcgtgcagg
      601 ttcccgtgca ggccggcggc gtgcccgtcg tcgtgaggaa gtggccgtcg cggccggcgt
      661 cgtcgagaaa cggccagttc gggtttggca ccgtgttctc gtacgcgttg acggcttggc
      721 actccgcgaa gaagtgcgtc gggtacgcga ggaacgcgcg cacctctgcg acgacctcat
      781 gaccgttgta cgtaatcttc gttccggcag cgcagagctg ctgcgtcgct ggacagttgc
      841 cgccgccgca cttcacggtt tcgcgatcgc cgacgttcca gtgcatcgac atgatctggc
      901 agtacgccgg tgtgccgtcc gccttgaaca gcgcgccgtt gtgatggtcg ttgttgggcg
      961 cgtcgcacgt gcccatgtca cccatgatgg cttcttcggt gagcatgtct gggttcgccg
     1021 tgcccgggcc acacgtgccg cacttcgcgg acggaaactc tttgccgttg ctctgcggaa
     1081 tgccggcggc gcggagatag cctgtcgcga tgtcctcggt gccgtcggcg aacaccgcga
     1141 ttgtcggcgc agcgatcatc tcgcgggcgg agttaccggt gaagctcgcg gtcgcttcgt
     1201 ggacgctcac gacatgaaag atcgtgcggg tcgcccacgg attcgcggcc cacttcgtct
     1261 tgtcgttcca gacgtcgatg atctgaagcg catgatcgtg atccgccgca tccaccacga
     1321 acggaccgcc gcggtacttg tgcgtgccga gcgaggtgcc ggggcgtgcg ctggtcgcgt
     1381 cgtcccagat cacgttcgtg gtcgccgtga agtcggggct cgcggtgggg taggcgcact
     1441 tcgcgctcgc gccctcgacg ccgcagtccc acgcgcagag atcgttcgcg gtgttgcagg
     1501 gcgcttggtg gtacgtcttg ttgggatcga tcacccagtg cacgtgcacg ccttgcttcg
     1561 tgagctggta gatcaggccg tacgcctgga acatcccgtg cgactggtag ctcagatcca
     1621 tcgggatgat caggctgccg gcgggaaacg tccgatcagc tgcgcgcgca gtaggcgcag
     1681 cgacgcacag cactgcgcca gcgatcacca aggcgatcgt tttgctcatg tctcattgtg
     1741 ccgccttccg tgggaaaggt tgcggcagcg ctcacaccca catcaaggcg tcacttcatc
     1801 gtcgtacatg agcgcactcg tgttctacga ggcgacggga agtggcggca cggtgaagaa
     1861 gatcgaagtg cccttgcccg gcgtgcttgt cgcccagatt cttccgccgt gcgcgtcgat
     1921 gatcgacttc gagatgtaca ggccgagacc gaggccacgc gggtcgccac cttccgattg
     1981 aaagtaccga tcgaagacct tctcgagcag ctccgtcgga attcctgacc ccgtatcgct
     2041 cacggagaac ccgatagcat cgtcgaggcg ctttgcccgg atgatgatgg taccgccgcg
     2101 ctccgtgaac ttgagagcgt tgccgacgag gttcgtgagt acctgcagga tgcgctggtg
     2161 atcgagctcg acgcttccga gattgccttc gggcgtgcat gtgagctcga ccccttcggc
     2221 ggttgcggcc ggttcgaacg cgtcatgcgc atcgcgcagc aactgctcca cgtcgtagcg
     2281 agcgcgaacg agcgagagct tccccgcatc gatactcacg acatccacga gatctccgac
     2341 gaggcgagtc attcgcgcgc tgaaccgttg gatgcgttcg gcgtagtgag taactttgac
     2401 aaagggctgc tccgacctct cgacgccctt gagcaagtcc gcgctcaatg cgatgccgcc
     2461 caatagactg cggagatcgt ggctgaccat cgccatgaaa tcttcacgcg acgcgagcac
     2521 ctgatcggcg cgtttgcgtt cgaggttgag ccggaggtcc gtatccttgc gctcgaatgc
     2581 gagcaagctc gcaagtgcaa tgcgctgcag ctcgacctcg tcgcgcgtgt ccgcgtcggc
     2641 gccgaggcgt tccgcgcgaa tgatcgcatc ctcgtgagct cgtgcatctg agacctgcgt
     2701 cgaagctccg gtttggacat cctcgcggtc gcgcgcttgg ttcaagacgg agtccgcctt
     2761 gccgcgcgcg tcgctgatcg cacctgcggt cgtctcgagc aatgacttcg atcgcgcagc
     2821 gagctcggcg tcggtcttct tacgctcgga ccgcaggccc tcgtcggttg agctgcgttc
     2881 ttgcggatct cgcttcgtcg tcatcgttgc gggcagtatg gatcccttat cgaaatggca
     2941 cgagccacgt ctccaacaca ttccgtggtg tgcgtcggcg ccacactctg aacgcgatca
     3001 gattgtgtcg attgtgtcgc ggttccgtta gggcacgtcg ccacagcgtt gcgtcatcat
     3061 gtcgtgcgag tagctcgagc cgacgagccc gcgtcgcgag atgcactccg cagtaccgcc
     3121 gctggcatcc caaccctgga attcatcgat ccggcaccat gcaccgtggt ggcgcaccat
     3181 cgcgtgagcg ttgtctccct cgaggttgct cggctcgtgg agaccccaat ccttcggcaa
     3241 tggcttcgac gtcggggacg gcgtgaagac cggttcgcca cggacgtcgt tgatgtaaag
     3301 cccgttggca gtcatcacgt aggtgttgat cgcgggggcc ggatcgcgga cgtcggggtc
     3361 cttcacgaac cagcacgcga tgcgcgctct cgtttcgtcg gccgtgacct cgaccgagtc
     3421 gacgtgacag cgctgcatgc tggcgggcag ctcgtcgtgg atgccgacac caaacgtgca
     3481 cggcaagtcc caggtcgccc cggcgacgaa cagggaggcg taaaatggtt gctcgggtgg
     3541 cggcgatgac tcgcgctgca ccgacgagct cgatggttcg atctggaagt cgagctggag
     3601 atcgagctcg agctgcggct cgtgaacatc caacgcagcg ctgctcggtg cctgttgctg
     3661 ttccgagcga tcgaacgtcc agattccgag ggcgacgacg actgcgacga cgcacgcgac
     3721 ggcgatggcc gaggtctgcc gcgtcgtgaa agcgatccgc acgatccgtc gacacgtctc
     3781 gagcgccgcg gtttcctacg gacgcgcgcc gaacgtaccg agcgagacgt atgcctcgtc
     3841 ttgcatgatc acgtaggggc gatcttcgaa cgtgaacgcc cgaccgtcga agcgcacgac
     3901 ctgcaggagg cccattccgt agcccattgg accttggcgg tagtagtgca gtccgacttg
     3961 gtagggaccc gcggttggcg taccgacgat ctcgaagcac tcggggccgt agcccgtggt
     4021 gacgtcggcg tagagctcgc cgcccgactc gagcgggaga tgcgagtacc aagagtggcc
     4081 gccgcgagcg tcgcggatgt gaaggtcgac gtcgttggcg tcggtctccc aatacagaag
     4141 gacgcgggtc gagcggccgg tgaatgcggt gagccgtcgc ttcgccagcg ccttctcgat
     4201 cgtggcgcgg tcgccgccgc cagcgagata cgcggatgcg atcatctgcg cgtcgcggcg
     4261 aaggacccgg tcagcgccgc gatagcgatc gtcggggtgc ttctgatcga cgcccttcaa
     4321 gatcgcgtcg aacgcacctg cgagatcgtt cgatcggacg agtgcgtagg caagcaatcg
     4381 atggcccgtg agctggtcag gccgatccgc gacggcgcgg cggtagctgt cgaccgcgag
     4441 cggccggccc gctgtctcga ggcgctcgag gcgctcgccg gcaaatcgcc ggtagtcggc
     4501 gcgcgtcgga tagagatcga tgagcgagcc gtagacgcgc gcggctgcgc tggtctcgtg
     4561 cgcagcttcg agcgcttcgc cgagcgcgac gattgcgaga acgtccgtcg gttgcgagtc
     4621 acgccacgca tgcgcttgct cgagcgcagt cggatcgtgc ttggcgatca ggcgcatgat
     4681 cgtggcgagg ttgccgcgca gcgcgctcgc ggcgctgttg aagcgcggga tcgtatcgtc
     4741 aggcctcgac accgtgacgc gtggttgcac gggggccggc gctggcaacg tgacgcgcga
     4801 atcgtcgtca gcagaatcga tcgccgtgct cggcgccgtg ctcggcgtcg cgatcgtcga
     4861 tgtcgtcggc gtggttgtcg gtggcgtggt tgtcgtcggc gtggttgtgg tcggcgtggt
     4921 tgtggtcggc gtggttgtcg tcggcgtgcc tgcgggcgcc acgctcgttg gcgcgctcgt
     4981 tgttggcgtc gtcgtcgtag tgggcgtcgt cgtcgttgtc gtcgtcggcg cagcagctgt
     5041 cgaagacgcg atcgtctgcg tcgcggttgg agtcgctggg ggcgctggca tcggcgcgga
     5101 cgtcggcggt ggggcaggtg acggagcggc ggcgcctgtc gattgctgca cgagacgctg
     5161 gatgtcgtcg ggcgcggcgt tgtcgtctgc aggcgtctcg gccgtgcgga acgtgaacgg
     5221 atagttcacg cgcacggtct cgtcgtgcga cgccggaaag tcgacgcggc gaagcacgtc
     5281 gacgatgcac tgcgcgactg ctgggtcgaa gcccgttgcg ctcgcatccg tcaccttgcc
     5341 gtccggtgcg atcgtgaagc tcgtcgcgat ctggccgtgc agcgacgggc gtgcgagcag
     5401 ctccttctcg tagcagtagc gaatgctgtt gaggtggcgc tgcagatagc ggcgcacaga
     5461 cgacttgtcg agcgagccca tcgccatcgg ctggccgatg ctcaccgtcg gcacacttgc
     5521 gacgcgaccg cgcaggccac cgcgcccgct gccgacgccg aagccggatc ccgtgccgct
     5581 gccgctgcca tgaccgacgg tgccgtagcg gcctgtcccg atcgtgcccc aaccgccgcc
     5641 gcccccaccc gcgccaaacc cgtgcctgcc gtagcccaac gtgccatcgg cctcttgatt
     5701 gcgatggccc ttacctccgc gaccgtcgcg atctttcgac gcgacgaagc ttcggtgctg
     5761 ttgctcgagc ccgtccttgc cgacgacgag gatgtcgacc agcgcattgc gatcgatgcc
     5821 gtagcgcgcg tactcgcgat cgttgtcgag gatgagcatc gtcgcttgcg acgacaccac
     5881 gcgcgc
//
LOCUS       DATLBZ010000039         7900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6722796, whole genome shotgun sequence.
ACCESSION   DATLBZ010000039 DATLBZ010000000
VERSION     DATLBZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6722796"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3029)
                     /locus_tag="VMZ53_01940"
     CDS             complement(<1..3029)
                     /locus_tag="VMZ53_01940"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HUS27230.1"
                     /translation="MLRAVHGNRVESLLDALLGALPPADPFAPTTIVVGSHLVSRWLT
                     RELAIARGIAAGLDLVTFDSFVERVWAGDEAAKAAKLVALDKAQLAASIASVLADDKL
                     VRSLSPVAAYLDAAPAPGDRAGPRRVQLAEHVAMLAWNYALTRPDWMPALLGGHVPEE
                     LRFDETTARTAAWQAALLGAAMQSAGCNEKLCSPVPTLPWARRRAKLAPPKLERPVFV
                     FGVSFLLRSQLEALTDLAQTTDVTIFLLDPCAELWDDVSGRAKAKAEASTSASASGQA
                     AGGATDPLPLTLWGRAIRDTLGALVERTGGDIDDAFTHAPPRTALELLLADVLRRQVA
                     ADMQLPPGTAAGLRVLACPDPRREIEIVAAEIRARLDADPKLHARDIGVWIASNSERY
                     LAQAPAAFEAVSVPCHLIDAPIDDRGRIAEAVLALLELPTGAMARRDLLRVMTHPALL
                     AGHPHVDAQDWVRWTEKLGIVHGADATAHRETYLAAHPGRFHWDQGVRRLALGAFMIG
                     GRDTSGLRERGAVRIGGMDVAPEEVRPEQHASAATYALMVRSLCSDSAWLGQHEATLT
                     QWATIFVGLVDAYIARPTDEASRDVERVRSMLAGLAHVDMDGRKVGFREAREHAAKRL
                     TEATSNRGEPLAAGVMLAPLRTMRALPFRHVFVVGLDEGAFPAGDQPSPLDVRKDARP
                     GDVSPRDRDRHAFFEALLGARDGLYLSYVAVEAKSGQALGPSSIVLELADALAPYLGA
                     GSSKAALDQLTTRFPLHRFAQDAAIPPAVQRERWAVRVRDALRAHLRGKGYPVPDEDG
                     MLALLADPHLADLRATLGIVEAPTVPPPAPPMRPLSLANVRKFLEAPVQAWAQAVLGL
                     DEVPDEETVEHSDEPFHVERPLRALLLREVLAAQLRDPGASAEERYKAVVRDLELRGQ
                     FPVGVFGDAARAADLRILQQWRDELGPMLLGTVGNDFLAQRIGAVGRLAFGRSSSPMA
                     ELMPALELSLASGRTVRLVGQTELLVRRGTGYASVIPL"
     gene            complement(3079..4017)
                     /locus_tag="VMZ53_01945"
     CDS             complement(3079..4017)
                     /locus_tag="VMZ53_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HUS27231.1"
                     /translation="MDPRTIVIVNPKSQGGRLGRQWIELSETLTRAFPYEHVETKGPG
                     DATKLAREALRSGADRVIAIGGDGTINEVVNGFFDEKGAAIKPEASFGLIPFGTGGDF
                     RRTIGIPLETVDAAAIIKANKTKKIDVGKLTLTTRAGESQLRMFINIASFGVSGVVDR
                     LVNESGKKLGRLSFMLATARATWSYTNQRVQLIFDGDPSTRVEATINTVAVANGKFFG
                     GKMKVAPHAEVDDGQFDVISLGDLTFADLLKSGRRLYEGTHLSMDKVSSRRARVVEAQ
                     PIEPGAIVELDVDGEAPGLLPAKFEIIAGGLTMVVP"
     gene            4113..5264
                     /locus_tag="VMZ53_01950"
     CDS             4113..5264
                     /locus_tag="VMZ53_01950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS27232.1"
                     /translation="MKLELVAPHSPRVEQIWRGLEDRAKPVYFLTWGWVENWLACLPA
                     LEAPQLAVINDDRGTPVAAAFLRRRWLRRHGVLPSRAVYLNTVGDHAYDEIYIEHNGL
                     LSTGDVTLARFVEQLEGAWDELFMPALEARAFAELADDFTTSAIGKRYRLRVDREVGD
                     HYVDLAKVRAKDYLSILGSSTRAQLRKAQRLAPDAVFEIAADETQAVAFYDELVELHG
                     RAWQAKGQSGAFADPWFDKFHRRLVAKRFAHGEIQLVRLRTPKETLGCLYNYVSHGRV
                     LVYQTGINPDVDKNLKPGFFTHLFAVEHNAKAGHATYDFLGGDAQYKKSLGTDETKLV
                     WARVQRKHLRFALEDRAREWVRARRARNETATEKTDAARPAAASARSSS"
     gene            complement(5227..6270)
                     /locus_tag="VMZ53_01955"
     CDS             complement(5227..6270)
                     /locus_tag="VMZ53_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS27233.1"
                     /translation="MTMDQARTTNSGAHAALVGVEVLVLDGDERVHAGIQTLLSEVRL
                     HVTSVRDPDEALDAAGRQFFSVAIIDIDTPSPRAGIETIKTLHKMSPTTMIVAMTPRR
                     SYDDAVDAVRAGAVDLILKAPESVAYLKERVLEAAGRSVGKREVDAILDEVRKVHDEF
                     LQRFMDAERKAMDLADKIAGRDESRLIQMDELSVLVVDEVDDFFTAMQATKTKGFRFV
                     HATNGGEALDRISTGEFQYAMIAQDITDLPAKTIAKTLRNQHPDTVVLIFLGPAENGH
                     VDLVEQTGMRSIVKPFTEAKQLIARLDELSEAWRIKAKERRYLQAFREKHYDFLRRFV
                     EIKTKIERSQRPA"
     gene            complement(6275..7486)
                     /locus_tag="VMZ53_01960"
     CDS             complement(6275..7486)
                     /locus_tag="VMZ53_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HUS27234.1"
                     /translation="MRCPSCGTENAQDSRFCGGCGAKLTPAAAAAPRLAPTAKIPDDA
                     PFPASTGQRPQVQAYQQGPASIPPPANPYGATGPASIPPAYGSAAPASIPPSRAPHVS
                     QPRAQSAVDSLSGRPTPMASELRAAAPRPGSEPAFGSIPPTQQRKPPRAVTPQPDGSM
                     PGTDDSFVAPPQRPLGLIAIVLVIDFALAGAGAVLLAKGLAKKPSEDASTPKAPELKM
                     QQRTEAAPMPPSSPTEAVAAAAPAPEPVVDVKADAKLPAAASALVETKSQARAKESTK
                     KSSSGAKTPTADKASSGPVTHPGGTAPEDPYKDQPDPSFDLKAESNRLASQSGDLFTR
                     CLNAAGAVHGTVKVAYRVDADGRVSHPFAVENSTGNEQLGICLSDVIATWRFSPHAGE
                     AQNAVRPFTYP"
     gene            7578..>7900
                     /locus_tag="VMZ53_01965"
     CDS             7578..>7900
                     /locus_tag="VMZ53_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27235.1"
                     /translation="MSQILDRVLTAARQLGASDVHLKAGLPPIFRIKGDLRTVRDVPA
                     LTREAIAQFAVHMMNDRQRADFETNLDIDLAYGTPDGVRYRVNLFQQRGSVGMVLRLI
                     PPEVPP"
BASE COUNT         1279 a         2671 c         2657 g         1293 t
ORIGIN      
        1 agcggaatca cggacgcata gccggtgcct cgacgtacga gcagctcggt ctgaccgacg
       61 agacgcacgg tgcgtccact cgcgagcgat agctcgagcg caggcatgag ctcggccatt
      121 ggcgacgacg aacggccgaa cgcgagccgg ccgacggcgc caatgcgctg cgcgaggaaa
      181 tcattgccga cggtgccgag cagcatcggg ccgagctcgt cgcgccattg ctggagaatg
      241 cgtaggtccg cggcgcgcgc ggcgtcgccg aacacgccga cgggaaattg cccgcgcagc
      301 tcgagatcgc ggacgaccgc tttgtagcgc tcttcggcgg acgcgcctgg atcgcgcagc
      361 tgcgcggcga gcacttcgcg gagcaacagc gcgcgcagcg gacgctcgac gtggaacggc
      421 tcgtcgctgt gctcgacggt ctcttcatcg ggaacttcat cgagaccgag caccgcttgt
      481 gcccatgctt gcaccggcgc ttcgaggaac ttgcgcacgt ttgcgagcga cagcggccgc
      541 atcggcggtg cgggcggcgg caccgtcggc gcctcgacga tgccgagcgt agcgcgcaga
      601 tctgcgagat gcggatcggc gaggagcgcg agcatgccgt cttcgtcggg aaccggatat
      661 cccttcccgc gcaggtgcgc acgcaacgca tcgcgcacgc gcaccgccca gcgctcgcgc
      721 tgcaccgccg gcggaatggc cgcgtcttgc gcgaaccgat gcagcggaaa ccgcgtcgtc
      781 agttgatcga gcgctgcctt ggacgatccc gcgccgagat acggcgcgag ggcatccgcg
      841 agctcgagca cgatcgacga cggcccgagc gcttggccgc tcttcgcctc gacggcgacg
      901 tacgagaggt agagcccgtc acgtgcgccg agcagcgcct cgaagaacgc gtgacgatcg
      961 cgatcacgcg gagagacatc gccgggacgc gcgtctttgc gaacatcgag cgggcttggc
     1021 tgatcaccgg cggggaatgc gccttcgtcg agaccgacga caaagacgtg gcgaaacggc
     1081 agcgcgcgca tcgtccgcag tggcgccaac atgacgcctg ctgcgagcgg ctcgccgcgg
     1141 ttagacgtgg cctcggtgag tcgcttcgcg gcgtgctcgc gcgcctcccg gaatccgacc
     1201 ttgcgcccat ccatatcgac gtgcgccaag ccagcgagca tcgaccgcac gcgctcgacg
     1261 tcgcgcgagg cttcgtcggt gggccgcgca atatacgcat caacgagccc gacgaagatc
     1321 gtcgcccact gcgtgagtgt cgcttcgtgc tggcccagcc acgcgctatc gctgcacagc
     1381 gagcgcacca ttagcgcgta ggtcgccgcg ctcgcgtgct gctccggtcg cacttcttcg
     1441 ggcgcgacat ccatgccgcc gattcgcacc gcgccgcgct cgcgcaaccc gctcgtgtca
     1501 cggcctccga tcatgaacgc gccgagcgct aaccgccgca cgccttgatc ccaatgaaac
     1561 cgacctggat gcgcggccaa gtacgtctcg cgatgcgccg tggcgtccgc gccgtgcacg
     1621 atgccgagct tctcggtcca ccgcacccag tcctgtgcgt cgacgtgcgg atgtccggcg
     1681 agcaacgcgg gatgggtcat cacgcgcaag aggtcgcgcc gtgccatggc accggtcggc
     1741 agctcgagca gcgcgaggac cgcctcggcg atgcggccac ggtcgtcgat cggcgcgtcg
     1801 atcaagtgac acggtacgct gaccgcctcg aaggcagccg gcgcctgcgc caagtaccgc
     1861 tcgctgttgc tcgcgatcca cacgccgata tcgcgggcgt gcagcttcgg atcggcgtcg
     1921 aggcgcgcgc gaatctcggc cgcaacgatc tcgatttctc gacgaggatc aggacacgca
     1981 agcacgcgta aaccggcagc cgtgccgggc ggtagctgca tgtcagctgc tacctggcgg
     2041 cgcagaacgt ctgcgagcaa cagctcgagc gcggttcgcg gcggagcgtg cgtgaacgcg
     2101 tcgtcgatat cgccgcccgt tcgctcgacg agcgcgccga gcgtgtcgcg aatcgcgcgg
     2161 ccccacagcg taagcggcag cggatcggtc gcgccgcccg cagcctggcc cgacgcgctc
     2221 gcgctcgtgc tcgcctcggc cttggctttg gcgcgccctg acacgtcgtc ccatagctcc
     2281 gcgcacggat cgagcaagaa gatcgtgacg tctgtcgtct gcgcgagatc cgtgagtgct
     2341 tcgagctggc tgcgcagcag aaacgacaca ccgaacacga acaccggtcg ctcgagcttc
     2401 ggcggcgcga gctttgcgcg gcggcgtgcc cacggcagcg tcggcacagg agagcagagc
     2461 ttctcgttac agcccgccga ttgcattgcc gccccgagca gcgccgcctg ccacgcagcg
     2521 gttctcgccg tggtttcgtc gaagcgcagc tcttcgggca cgtggccacc gagcagcgcg
     2581 ggcatccaat cagggcgcgt gagcgcgtag ttccacgcga gcatcgcgac atgctcggcg
     2641 agctgcacac ggcgcgggcc cgcgcgatcg ccgggcgcgg gtgcagcatc gaggtatgcg
     2701 gcgacgggcg acagcgatcg cacgagcttg tcgtcggcga gcaccgatgc gatcgatgcg
     2761 gcgagctgcg ccttgtcgag cgcgacgagc tttgcggcct tcgctgcctc atcgcccgcc
     2821 cacacgcgct cgacgaacga atcgaacgtg acgagatcga ggccggctgc gatgccgcgc
     2881 gcgatcgcga gctcgcgcgt gagccaccgc gagacgagat ggctaccgac gacgatcgtg
     2941 gtcggcgcga acggatcggc cggcggcaac gcgccgagca gggcgtcgag cagcgactcg
     3001 acgcgattgc catgcaccgc gcggagcact agacaccgcc tgctacgggc ggcacatgcc
     3061 gctgcggcgc gcgcgtgctc acggcaccac catcgtgaga ccgccggcaa tgatctcgaa
     3121 cttcgcgggg agcaggcccg gcgcttcgcc gtcgacgtcg agctcgacga tggcgccggg
     3181 ctcgatcggc tgcgcttcga ccacgcgtgc gcggcgcgag ctcaccttgt ccatcgatag
     3241 atgcgtgcct tcatagaggc ggcgcccgct cttcaataga tcggcgaacg tcaggtcgcc
     3301 cagcgagatg acgtcgaact ggccgtcatc gacttcggcg tgcggcgcga ccttcatctt
     3361 gccgccgaag aacttgccgt ttgcgaccgc caccgtgttg atcgtggctt cgactcgcgt
     3421 cgacggatcg ccgtcgaaga tgagctgcac gcgctggttc gtgtacgacc acgtcgcgcg
     3481 cgcggtggcg agcatgaatg acaggcggcc gagcttcttg ccgctctcgt tgacgagacg
     3541 atcgaccacg ccggagacgc cgaacgacgc gatgttgatg aacatccgca gctgcgattc
     3601 gcccgcgcgc gtggtgagcg tcagcttgcc gacatcgatc tttttcgtct tgttcgcctt
     3661 gatgatggca gcggcgtcga cggtctcgag cgggatgccg atcgtgcggc ggaaatcgcc
     3721 gccggtgccg aacggaatga ggccgaagct ggcttcgggc ttgatggcgg cgcccttctc
     3781 atcgaagaag ccgttgacca cctcattaat ggtgccgtcg ccgccgatcg cgatgacgcg
     3841 gtccgcgccc gatcgcagcg cctcacgcgc gagcttggtc gcgtcgcccg ggcccttcgt
     3901 ctcgacgtgc tcgtagggga acgcgcgcgt cagcgtctcc gacagctcga tccactgccg
     3961 gccgagtcgc ccgccttgcg acttgggatt gacgatgacg atggtgcgcg ggtccacgtc
     4021 tcgcagcata tcgtgtgagt ccgacagtct tcgtcggtga tggccgctcc cgggtttgat
     4081 cccagtagtt agatgctagg gtccgagcac gcgtgaagct cgagctggtt gccccgcatt
     4141 cgccgcgcgt cgagcagatc tggcgcggcc tcgaggatcg cgcgaagccc gtgtattttc
     4201 tgacgtgggg ctgggtcgag aactggctcg cgtgcttgcc tgcgctcgaa gcgccgcagc
     4261 tcgccgtgat caacgacgac cgcggaacac cggttgcggc ggcgtttctg cgtcgccggt
     4321 ggctgcgccg ccatggcgtg ctgccgagcc gcgcggtcta tctcaatacc gtcggtgacc
     4381 acgcctacga cgagatctac atcgagcaca atgggctgct ctcgacgggc gacgtaacgc
     4441 tcgcgcgctt tgtcgagcag ttggagggag cctgggacga gctgttcatg cccgcgctgg
     4501 aagctcgcgc gttcgcggag ctcgccgatg acttcacgac cagcgcgatc ggcaagcgct
     4561 accggctgcg cgtcgatcgc gaggtcggcg atcattacgt ggacctcgcg aaggtccgcg
     4621 cgaaggacta cctctccatc ctaggcagct cgacgcgcgc gcagctccgc aaggcgcagc
     4681 gcctcgcgcc cgacgccgtc ttcgagatcg ccgccgacga gacgcaggcc gtcgcgttct
     4741 acgacgagct cgtcgagctg cacggccgcg cgtggcaagc gaaaggccag tccggcgcat
     4801 tcgccgatcc atggttcgac aagttccatc gccgcctcgt cgcgaagcgg tttgcgcacg
     4861 gcgagatcca gctcgttcgc ctgcgcactc cgaaggaaac gctcggctgc ctctacaact
     4921 acgtctcgca cggacgcgtg ctcgtctatc agaccggcat caatcccgac gtcgacaaga
     4981 acctgaagcc cggcttcttc acgcatctct tcgcggtcga gcacaacgcg aaagcgggcc
     5041 acgcgacgta cgacttcctc ggtggcgatg cgcagtacaa gaaatcgctc ggcaccgacg
     5101 agaccaagct cgtgtgggcg cgcgtgcagc gcaaacacct gcggtttgct ctcgaggatc
     5161 gcgctcgcga gtgggtccgc gcacgcagag ctcgcaacga aaccgcgacc gagaagaccg
     5221 acgccgctag gccggccgct gcgagcgctc gatcttcgtc ttgatctcga cgaagcggcg
     5281 caagaagtcg tagtgcttct cgcggaacgc ctgcaggtag cggcgctcct tcgccttgat
     5341 ccgccacgct tccgacagct cgtcgagccg cgcgatcagc tgcttggcct cggtgaacgg
     5401 cttcacgatc gatcgcatgc ccgtctgctc gacgagatcg acgtgaccgt tctccgccgg
     5461 accgaggaag atcagcacga ccgtatcggg atgctggttg cgcagcgtct tcgcgatcgt
     5521 cttcgcggga aggtcggtga tgtcctgcgc gatcatcgcg tactggaatt cgccagtcga
     5581 gatccgatcg agcgcctcgc cgccgttcgt cgcgtggacg aagcggaacc ccttcgtctt
     5641 cgtcgcctgc atcgcggtga agaagtcgtc gacctcgtcg accacgagca ccgagagctc
     5701 gtccatctgg atcagccgag actcgtcgcg gcctgcgatc ttgtctgcga gatccatcgc
     5761 cttgcgctcg gcatccatga agcgctggag gaactcgtcg tggactttgc ggacctcgtc
     5821 gaggatcgca tcgacctcgc gcttgcccac ggagcgcccc gctgcttcga gcacgcgctc
     5881 cttgagatac gcgaccgact ccggcgcctt gagaatcaga tcgacagcac cggcgcgcac
     5941 cgcgtcgact gcgtcgtcgt acgagcggcg cggtgtcatc gcgacgatca tcgtcgtcgg
     6001 tgacatcttg tgcagcgtct tgatcgtctc gatgcccgca cgcggcgacg gcgtgtcgat
     6061 atcgatgatc gccaccgaga agaactggcg gcccgcggcg tcgagcgctt cgtccggatc
     6121 ccggacgctc gtaacatgga gccgaacctc cgagagcagc gtttggatac cagcatgcac
     6181 gcgctcgtcg ccatcgagca cgagcacctc gacgccgacg agcgccgcgt gagcgccgga
     6241 attggtagtg cgggcctggt ccatcgtcat gaggtcacgg gtaggtaaac ggtcgtactg
     6301 cattctgcgc ctcgcccgcg tgcggagaga agcgccaggt cgcgatcacg tcggagaggc
     6361 aaatgccgag ctgctcgtta ccggtcgagt tctcgacggc aaaaggatgg ctgacgcgtc
     6421 cgtcggcgtc gacgcggtag gccactttga cggtgccgtg aaccgcgccg gccgcattga
     6481 gacagcgcgt gaacaagtcg ccgctctgcg acgcgagccg gttggactcc gccttgagat
     6541 cgaagctcgg gtcgggctgg tctttgtaag gatcctcggg cgcggtgcca ccgggatgcg
     6601 tgacgggccc gctcgatgct ttgtcggccg tcggcgtctt cgcccccgac gagctcttct
     6661 tcgtcgactc tttcgcgcgc gcttgcgact tcgtttcgac gagcgcggat gcagccgcgg
     6721 gaagcttcgc gtcggccttc acgtcgacga ccggttccgg cgctggtgcc gctgcagcaa
     6781 cggcttccgt cggagacgac ggcggcatcg gcgcggcctc cgtgcgctgc tgcatcttca
     6841 gctcgggagc cttcggcgtg ctggcatcct cgctcggctt cttcgcgagc ccctttgcga
     6901 gcaacaccgc gccggcaccg gcgagcgcga aatcgatcac gagcacgatc gcgatgaggc
     6961 cgagcggacg ctgcggcggc gcgacgaacg aatcatctgt gcccggcatc gagccatcgg
     7021 gttgcggcgt gacggcgcga ggcggctttc gctgctgcgt gggcgggatg ctcccgaagg
     7081 ctggctccga acccggacgc ggcgctgcag cacggagctc ggaggccatc ggagtcggcc
     7141 gccccgagag cgagtcgacc gccgactgcg cgcgcggctg cgagacatgc ggcgccctcg
     7201 acggcggaat cgacgcgggc gcggccgagc cgtacgccgg cgggatcgag gcgggaccgg
     7261 tcgcgccgta tgggttggca ggcggcggaa tcgaagcggg gccctgttga taggcctgga
     7321 cctgcggacg ctgacccgtc gaggctggga acggtgcgtc atcggggatc ttggccgtcg
     7381 gcgccaggcg cggggccgcg gccgctgcgg gcgtcagctt ggcgccgcaa ccaccgcaga
     7441 accgggaatc ctgcgcattc tcggtgccgc agctagggca acgcatcccg ccatctaggg
     7501 taactcaggg ccgacgacgg cgcctcatct gcagcaggag atccagcggt gaccgtgtgt
     7561 taccctagat cctggcgatg agtcagatcc tcgatcgcgt cctgaccgcc gctcgccagc
     7621 ttggcgcttc tgacgtgcac ctgaaggcgg ggttaccccc gatcttccgt atcaagggtg
     7681 acctgcggac cgtccgcgac gttcccgcgc tcacgcgcga ggccatcgcc cagttcgccg
     7741 tgcacatgat gaacgaccgg caacgagcgg acttcgaaac caacctcgat atcgacctcg
     7801 cgtacggcac gccggacgga gttcgctatc gcgtcaacct gttccagcaa cgtggatcgg
     7861 ttggcatggt cctgcgtttg atccctcccg aagtgccgcc
//
LOCUS       DATLBZ010000040         4774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3757999, whole genome shotgun sequence.
ACCESSION   DATLBZ010000040 DATLBZ010000000
VERSION     DATLBZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3757999"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VMZ53_01970"
     CDS             <1..276
                     /locus_tag="VMZ53_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27236.1"
                     /translation="FTYDVYFTFPSPDMRAELWRRTLPQGSSSKIDYDSLAEKFELTG
                     GFIKVACERAAYVAGANGTNIDEELLRATVERMYRERGKLSTVGPLE"
     gene            273..905
                     /locus_tag="VMZ53_01975"
     CDS             273..905
                     /locus_tag="VMZ53_01975"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HUS27237.1"
                     /translation="MTASFDRRGMYEITWGTLRAIEPTRAELVQCSAALAEGYNDPVN
                     APLMGHTSVITPSEVVESYEESIANGMRAFLFFVGDEFAGDGDLRNPRGTPGESNALR
                     MSRGHSAEFAFMIATPSAQGKGLGTRFATMLHMFGFREICLARIYASVVPQNTASLRA
                     FEKLGYKRDDSPEARAYAEEPGDITLSVLREPFLDQHRALYDEIRIGVRK"
     gene            902..1255
                     /locus_tag="VMZ53_01980"
     CDS             902..1255
                     /locus_tag="VMZ53_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HUS27238.1"
                     /translation="MTAASNVLTGVVAFLHVYFLVLEMFLWAKPFGMKTFKRTREEQE
                     STKILAANQGLYNGFLAAGLVWSYWAGCDVRVFFLACIVVAGIYGAATAARSILFAQA
                     IPGAAALVVTLLACR"
     gene            complement(1257..1607)
                     /locus_tag="VMZ53_01985"
     CDS             complement(1257..1607)
                     /locus_tag="VMZ53_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27239.1"
                     /translation="MPRVSKAAMKPGTERYRDERRNDPKPKMHGKQWHDHAENPVEGT
                     QGSAYGSSVKGPVRDGGETTGLGKTKRGTKGAQRKRMTPVSKGRTGERAKAKAGAGRN
                     VKTGMKVKGRRTRA"
     gene            complement(1650..2402)
                     /locus_tag="VMZ53_01990"
     CDS             complement(1650..2402)
                     /locus_tag="VMZ53_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HUS27240.1"
                     /translation="MKIATWNVNGIRARSSQVVHWIDGEEPDVICLQEIKASPHQVPE
                     PLAQIAGYHAHWHGSGGYSGVALMLRIGRFPEPPKFVHPPFDLECRIVVAEVGSIVFG
                     SVYIPNGGKDFQAKLVFLREIADWAGALEKAGKHLVLCGDFNVARAEVDVHPVLRKDM
                     VGQSKIERGLIDELFSHGLVDVGRHVDPDNDRLFTWWAPWRNMRQRNMGWRLDYVAAH
                     HALVDGAAACKVHRDVGTSDHGPVIAHIKDAP"
     gene            complement(2421..3488)
                     /locus_tag="VMZ53_01995"
     CDS             complement(2421..3488)
                     /locus_tag="VMZ53_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27241.1"
                     /translation="MRALALVFLAACGGGITADAQPVVSVLKQDGGCFALMTDDTPVS
                     STLGVSGTCNYKAAPRLLAGVDLIELVIDYGEDVDFAGTATAPTPKVDVTVDGVQSEV
                     PVEVSREQRVGSRAYFIATFYAPTRISSDVRITVGVNSGFQTLVPVVFTTIAPPVSLS
                     LFECPQGTQCELAGAVGSAHIALAVPGTVPQSIVIHSLLNGVPQPDPIPPVRTAPAQG
                     NTEAVTAIPVPAAPDFTQWTIRAQLGESTPVQVFATIRAPELHALLTCPSGCSTLGTD
                     EPAGLTIIAPAQIRPLQARVDTLIDGVPQVIAAPVELVQNADGTAQGLLALRSPSTTG
                     EWQINVTVAGYQAPAIVTTIQ"
     gene            complement(3485..>4774)
                     /locus_tag="VMZ53_02000"
     CDS             complement(3485..>4774)
                     /locus_tag="VMZ53_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27242.1"
                     /translation="GGTTGIVEALDHMSDRFVDKTNVMTPVQSAVWAEVGSDPFAAAV
                     AIMLARSGALLPQVKAKASAPRGGASGGGGVALFTADGQLEVKAVQLLDDGRGLRVAS
                     SVAPKGEGQARVLASGTDSLMVSDGLTLEDLVQLGERHVRVDGEWVTLRSLGDRVSAI
                     GQDSWNADDAAYLAAATSQMKRLADFVDLTTTGATCARSTYTAELTSDAVRKHLGEWL
                     ACQKADSAALEAMLEQLELLANEHQAWKQSKEKLVAASRRIVTLTTTAPENLRVSFRS
                     RTWVFSYVTPIVGYAGVLRPDETFGLFYLGAQIHLDPNPVDDVQWRHGVTAKDLRRAV
                     ALELGVAPYRSNVGPDMRYGGPGALPPLFVGAAVHILPYTSLTFGAAFVDRRHSTLPQ
                     EQPHTVVTPYLGFTIQLNVPDLIRQVSGTTSDTETSR"
BASE COUNT          813 a         1606 c         1513 g          842 t
ORIGIN      
        1 ttcacgtacg acgtctactt cacgttcccg agccccgaca tgcgcgcgga gctgtggcgc
       61 cggacactgc cacaaggaag ctcgtcgaag atcgactacg actcacttgc cgagaagttc
      121 gagctgaccg gtggcttcat caaggtcgcg tgcgaacgcg cggcgtacgt ggcgggtgcg
      181 aacggcacga acatcgacga ggaattgctg cgcgcgactg tcgagcgcat gtaccgcgag
      241 cgcggcaagt tgagcaccgt aggcccgctc gaatgacagc atcgttcgat cgccgcggca
      301 tgtacgagat cacgtggggc acgctccgcg cgatcgagcc gacacgcgcc gagctcgtgc
      361 agtgctccgc cgcgctcgcc gagggctaca acgacccggt caacgcgccg ctcatgggcc
      421 atacgtcggt gatcacaccg agcgaggtcg tcgagtcgta cgaggaatcg atcgcgaacg
      481 gcatgcgcgc gtttctattc ttcgtcggtg acgagtttgc gggcgacggc gaccttcgca
      541 atccgcgcgg cacgccgggc gaatcgaatg cactgcgcat gtcacgcggt cactcggcgg
      601 agtttgcctt catgatcgcg acaccgtccg cacagggaaa aggcctcgga acgcggttcg
      661 cgacgatgct ccacatgttc ggctttcgcg agatctgcct cgctcggatc tacgcgtcgg
      721 tcgtgccgca gaacaccgcg tcgctgcgtg ccttcgaaaa gctcggctac aagcgcgacg
      781 actctcctga agcgcgcgca tatgccgagg agccgggcga catcacgttg tccgtcttgc
      841 gcgagccctt cctcgatcaa caccgagcgc tctacgacga gattcggatc ggagtccgca
      901 aatgactgca gcctcgaacg tgctcaccgg cgtcgtcgcg ttccttcacg tctacttcct
      961 cgtgctcgag atgttcctgt gggcgaagcc cttcggaatg aagacgttca agcgaacgcg
     1021 cgaagagcag gaatcgacga agatcctcgc ggcaaaccag ggcctctaca acggctttct
     1081 cgccgcgggc ctcgtctggt cgtactgggc aggctgcgac gtccgcgtgt tctttctcgc
     1141 gtgcatcgtc gttgccggca tctacggcgc agcgaccgcg gcgcgaagca tcctattcgc
     1201 gcaggcgatt cccggcgcgg ctgctctcgt cgtgacgctg ctcgcttgtc gctaggctag
     1261 gcgcgggtcc ggcgaccctt gaccttcatg ccggtcttca cgttgcgacc ggcacccgcc
     1321 ttcgctttcg cgcgctcgcc ggtgcggccc ttcgagaccg gcgtcatgcg cttgcgctgc
     1381 gcgcccttgg tgccgcgctt cgtcttgcca aggcctgttg tctccccgcc gtcgcgcacg
     1441 gggcccttca ccgagctccc atatgccgag ccttgcgtgc cctcgacggg attctcggcg
     1501 tggtcgtgcc actgcttgcc gtgcatcttc ggtttcggat cgttgcgacg ctcgtcgcga
     1561 tagcgttccg ttcccggctt catcgctgct ttgctgacac gaggcatacc tcatagcgaa
     1621 gcaagcgacg tgccctgaga attcctcgtc tagggtgcgt ccttgatatg cgcgatgaca
     1681 ggcccgtgat cactcgtgcc gacgtcgcga tgcaccttgc acgcggctgc accgtcgacg
     1741 agtgcgtggt gtgcagcgac gtagtcgagc cgccagccca tgttgcgctg gcgcatgttg
     1801 cgccagggcg cccaccacgt gaaaagccga tcgttatccg gatcgacgtg ccgtccgacg
     1861 tcgacgagcc cgtgcgaaaa cagctcgtcg atgaggccgc gctcgatctt cgattggccg
     1921 accatgtctt tgcgcagcac aggatgcacg tcgacctcgg cgcgcgcgac attgaagtcg
     1981 ccgcacaaga cgaggtgctt gcccgccttc tcgagcgcgc cagcccaatc cgcgatctcg
     2041 cgcaggaaga cgagcttcgc ctgaaaatct ttgccgccgt tcgggatgta gacggagccg
     2101 aacacgatgc tgccgacttc cgcgacgacg atccggcact cgaggtcgaa cggcggatgc
     2161 acgaacttcg gaggctcggg gaagcgaccg atgcgcaaca tcagcgcgac gcccgagtaa
     2221 ccgccgctgc cgtgccagtg cgcgtgatag ccagcgatct gcgcgagcgg ctcgggaact
     2281 tgatgcggcg acgctttgat ctcctgcagg cagatcacgt ccggctcctc gccgtcgatc
     2341 cagtgcacga cttgcgagga ccgcgcgcga atcccgttga cgttccaggt cgcgatcttc
     2401 acgaccgagc gtgtagcaga ctactggatg gtcgtcacga tggccggtgc ctgataaccg
     2461 gcgacggtca cgttgatctg ccactcgccc gtcgtcgatg gtgaccgcag tgcgagaaga
     2521 ccttgcgcgg tgccatcggc attctggacg agctctacgg gcgcggcgat cacctgcggc
     2581 acgccgtcga tgagcgtatc gacacgcgct tgcagcgggc ggatctgcgc gggcgcgatg
     2641 atcgtgagac ccgcgggctc gtcggtcccg agcgtcgagc atcccgacgg acacgtgagc
     2701 aacgcatgga gctcgggcgc gcgaatcgtc gcgaacactt gcaccggcgt gctctcaccg
     2761 agctgcgcgc gaatcgtcca ttgcgtgaag tcaggcgcgg caggcacggg aatcgcggtg
     2821 accgcttcgg tgttgccctg cgcgggcgcc gtcctgacgg gcggaatcgg atcgggctgc
     2881 ggcacgccgt tgagcaacga gtggatgacg atcgactgcg gcaccgtgcc gggaaccgcg
     2941 agcgcgatat gcgcgctacc gaccgctcca gcgagctcgc attgcgtgcc ctgtgggcac
     3001 tcgaacaacg acagcgacac aggcggcgcg atcgtcgtga acacgaccgg gacgagcgtt
     3061 tgaaatcccg agttcacgcc gaccgtgatg cgcacgtccg acgagatccg ggtcggcgca
     3121 tagaacgtgg cgataaaata cgcgcgactt ccgacgcgct gctcgcgaga cacctcgacg
     3181 gggacctcgc tctgcactcc atcgacggtg acgtcgacct tcggcgtcgg cgcggtcgcc
     3241 gttcccgcga agtcgacatc ctcgccgtaa tcgatgacta gctcgatcag atcgacgccc
     3301 gcgagtagac gcggtgctgc cttgtagttg cacgtgcccg agacgccgag cgtcgacgag
     3361 acgggcgtgt catcggtcat gagcgcgaag cagccgccgt cttgcttgag cacggagacg
     3421 acgggctgtg cgtccgcggt gatgccgccg ccgcacgcgg ccagaaacac aagtgccagg
     3481 gctctcatcg gctcgtctcc gtgtcgctcg tcgtgcccga gacctggcga atgaggtccg
     3541 gaacgttgag ctggatcgtg aatcccaagt aaggcgtgac gacggtatgc ggctgctcct
     3601 gtggcagcgt gctgtgtcgc cgatcgacga acgccgcgcc aaacgtcagc gacgtataag
     3661 gaaggatgtg caccgccgcg ccgacgaaca gcggaggtaa tgcaccgggc ccgccgtatc
     3721 gcatgtcagg cccgacgttc gagcgatagg gcgcgacgcc gagctccagc gcgaccgcgc
     3781 gtcgaagatc cttcgcggtg acgccgtgtc gccattgcac gtcgtcgacc ggattgggat
     3841 cgagatggat ctgcgcgccg agatagaaga gaccaaacgt ctcgtcggga cgcagcacgc
     3901 cggcataacc gacgatgggc gtcacgtagc tgaacaccca cgtgcgtgag cgaaacgaca
     3961 cgcgcagatt ctcgggtgcc gtcgtcgtca aggtaacgat ccgtcgcgac gcggcgacga
     4021 gcttttcctt cgattgcttc cacgcttgat gctcgttcgc gaggagctcg agctgctcga
     4081 gcatggcttc gagcgcggcc gagtcggcct tctgacacgc gagccattcg ccgagatgct
     4141 tgcgcaccgc atcgctcgtg agctccgccg tatacgtgct gcgcgcgcag gtcgcgccgg
     4201 tcgtcgtcaa atcgacgaag tcggcgagcc gcttcatctg cgacgtcgct gctgccagat
     4261 acgccgcgtc gtctgcattc cacgagtcct gcccgattgc cgacacgcga tcgccgaggc
     4321 tccgcagcgt cacccactcg ccatcgacgc gaacatgccg ctcgccgagc tgcaccaaat
     4381 cctcgagcgt caacccatcg ctgaccatga gcgagtcggt cccgctcgcg agcacgcgcg
     4441 cctgcccctc ccccttcggc gccaccgacg acgcaacgcg cagcccgcgt ccatcatcga
     4501 ggagctgcac cgctttcacc tcgagctggc cgtcggccgt gaacagcgcc acgccgccac
     4561 cgccgctcgc accgcctcga ggcgccgacg ccttcgcctt tacctgcggc aacagcgcgc
     4621 cgcttcgcgc caacatgatc gcgacggccg ctgcgaatgg atcgcttcca acttccgccc
     4681 acaccgcgct ctgcacgggc gtcatcacgt tcgtcttgtc gacgaaccgg tcgctcatgt
     4741 gatcgagcgc ttcgacgatt ccggtcgtac cgcc
//
LOCUS       DATLBZ010000041        21794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4502878, whole genome shotgun sequence.
ACCESSION   DATLBZ010000041 DATLBZ010000000
VERSION     DATLBZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21794
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4502878"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..878
                     /locus_tag="VMZ53_02005"
     CDS             54..878
                     /locus_tag="VMZ53_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUS27243.1"
                     /translation="MAEITIAVVPSATPGDDSGFVLDGLCVALTKLLDVPVSGINSRS
                     YQDLAAELEKGRVDYAWMSPTLMLLTAEHIQLRPLLSAVRNGSTEYCSALFVDDAMPI
                     DEIDDLHNSTVAWVDSTSASGYLFPRLMLAAQGKDPAHLFRKELFLRSHAEVVRAVLD
                     GKADVGATYAQRPVEGEPVTRAGWLHVAPTRSVRVLEYSQPIPNDMIVGHGLIEKAHH
                     TDFVNAILTLSNLENGKRLLYRAFHAEQFKATPRNALRPLEMLLQTARSHGLFSHL"
     gene            complement(875..1780)
                     /locus_tag="VMZ53_02010"
     CDS             complement(875..1780)
                     /locus_tag="VMZ53_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27244.1"
                     /translation="MFRLGLAAALAASACGRFGFDPGNTRDDAMVVSTDSSIDAPPPD
                     ALPPMGPFGTAMPVPGLNTAASEDDPSLTGDQLEIVFNSDRAGGVGAGDMWTASRPSL
                     TSGWTGVTNLSTLNTAGDDATPALSRDGLTLLWTAAGSKGMKDIFMATRPSRTAAWSA
                     AQELTAISTTGDDAGPFLSADGLELYFASDAPGNDNLYVARRTTTTAAFTTAAQITEL
                     NTTSPDHEPWVNGTKSFLVWTSTRVSGNDDLWMARRADASATWGTAAPITELNTASTE
                     SDPWLSPDETTIYFARGTDIFMASR"
     gene            1803..2498
                     /locus_tag="VMZ53_02015"
     CDS             1803..2498
                     /locus_tag="VMZ53_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HUS27245.1"
                     /translation="MARRHRERHRSGRTGWLRAAVLGSNDAIVSTAGLMMGVAATDAS
                     TQTILIAGIAGLCAGSMSMAAGEFVSVSSQRDAEQADIAKETRELEEEPEAELKELAG
                     IYHSRGLDKALAMQVAKQLSAHDRLGAHLRDELGIEPNNRARPLQAAWISAVSFALFA
                     LVPIIALLIAPKNFRMETIAIITLVSLAALGALGGKLGGAPLVRAALRVTFGGALALA
                     VTYGIGRLLGLTL"
     gene            2510..2794
                     /locus_tag="VMZ53_02020"
     CDS             2510..2794
                     /locus_tag="VMZ53_02020"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HUS27246.1"
                     /translation="MCRNIRVLHYFKPPTTHEEVHAAALQYVRKVSGMRDPSDEAAKA
                     FEKAVEQIAAATSKLLGVLPKKGEPRTREGEKLKAKARWERREKQIRSAE"
     gene            3055..4779
                     /locus_tag="VMZ53_02025"
     CDS             3055..4779
                     /locus_tag="VMZ53_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HUS27247.1"
                     /translation="MRYHALAADYDGTLAHHGLIDDLTWASLKQLRESGRKLIMVTGR
                     ELDELLALLPHPEMFDRIVAENGAVLYSPASKKVTLLAPPPPPEFGDELKKRGAERVA
                     VGRVIVATWEPHQDTVLHVIHDMGLELQVIFNTGAVMILPTGVNKATGLEAALHDLGL
                     SKHNVVAVGDAENDHALLTRAELGVAVANALPSLKQHADLVTPGDHGTGVSQVIDRLI
                     ANDLADISPPRHRIKVGQANGQHVTIDPYETNIMIAGTSGSGKSTLTTAILERLCERG
                     YQYVILDPEGDYTSIESAVVLGAPHRAPLVDEVIDVLRDSSRNAVVNMLGIAVDHRPE
                     FFAKLLPALAELRMRTGRPHWLVVDEAHHLLPSSWQPVAELALRPHGTLYVTVHPGSV
                     AQPVIDTINTVFVVGDHPDKTVKEFWNVARIKKPPHVASEKLGPGHALYWETKTNLAA
                     VIETEPSTTERTRHSRKYVEGNLGSHSFVFRGPDKKLNLKASNLMLFLLLAEGVDDDT
                     WLHHLRNGEYSQWFRTNVKDPDLAQAAEAAEKDASISAQASRELIRAEIEKRYTLPAD
                     EPSGVIDD"
     gene            complement(4734..5819)
                     /locus_tag="VMZ53_02030"
     CDS             complement(4734..5819)
                     /locus_tag="VMZ53_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HUS27248.1"
                     /translation="MKSDPVLELLPLGFQWQTFDPFLFCVHHDDAYPAGNDDLGPAAS
                     LDGRDIGQDFAGKDGWRMYHGDTVPGFPGHPHRGFETVTIVRRGLIDHSDSLGAAARF
                     GRGDVQWLTAGKGIVHSEMFPLLDKSGGNPVELFQIWLNLPRASKMVNPYFSMLWDHT
                     IPRHVARDSQGRTTEVVVVAGTLGETKPLPAPPDSWASRADADVAIWSIKMAPNAEWT
                     LPAAKAGTNRTLYFYAGDRLTLGEKSLVKHAGARLRPEAEVQLVAGKDGVEILVLQGK
                     PIGEPVVQHGPFVMNTAAEIQQAFLDFRRTQFGGWPWPKGDPTHGKTEGRFARHADGR
                     VEKVDEPPAKATPQSSMTPLGSSAGSV"
     gene            5995..7674
                     /locus_tag="VMZ53_02035"
     CDS             5995..7674
                     /locus_tag="VMZ53_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27249.1"
                     /translation="MRLLYLAPMFALVACGGDQLDDGYGYGAPDAGYSSDAGFVGCLS
                     SNECAPGYYCNDFGRCEKQATGSGDGGMPTPPPEKEYEFAPPTSSQRFVYVAMTAQDE
                     LARIDGATLAVKSTKVGKSPKVVATIPGTDGAVVLDSFNGTATVVRPVGDTDSTRVLG
                     TLQRLNRLDIDPTGRFAVIWFDLAKAIKEGNTFSVGSFQDVTVVRLAAGNEKAVNLTV
                     GFRPREVQFDAAGNRAYVITQDGVSVIDLGYATDHTATIIPPIAVADPSISPDDLEVD
                     VVSTGEWAAVRQTNSATLRVVNVGSSPGTAYSVTLASPATDIDLAPNGARLYAVQRAA
                     KKLSVVDIPGDAQNPSGVETIDLTNATVGSLTLSLDGKRGLMFTNATSDERVTLIKLD
                     QPGYPQSTWPIKKSVRAVAVSPSGDTALLINAKMAGDPATASTFDDFVDKSYGYTLLD
                     LASGFGKLQITPVDPGAFTYAPDGTKVYVALDGGDAPTATRAIQVLTTQTGVVQTKTL
                     GSPPSSVGILPGAAQAFVNQRHPLGRVSFVDLINDAQRTVTGFDLNSHIVN"
     gene            7684..9276
                     /locus_tag="VMZ53_02040"
     CDS             7684..9276
                     /locus_tag="VMZ53_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27250.1"
                     /translation="MKTMLLALAALAALPACWLGEDERPIVWDRERHVLGPIPMKTQI
                     AYVDSALDRVTLLDLANAQPEISTQKIGRRAVVAVPSPDRHLLFVITRGEEAIHEGEI
                     DQAPMFWVIDTSNRTPPTAYEIGSPFDRVAVAPDNSMVVCYFSAAGPDEAGFFRNPNE
                     LAIIHLTEAPSDSNPTLRTIRSFGSTPEGVTLSPPMIVPGAADGEPRTFAYVLSSNNL
                     TLVDISHPDRREMSIRLDLGGSAVLPKEVVFAPHTATAYVRSDNARDVLQVLLEAVPP
                     TEANANDFRPVLAELGAGGGPTDIAVYDDPSGRRYVLAATPNTKEVVVIDADTAQFRS
                     VPVPDAIDRILLFPVGVDTPPTKALFASVAAKSTKVNVLDLVHIEDPLTQASMRTITL
                     DKPVRDVVPVPGRDLAMLVHDDARTVLGLLDMDTESTSPLLGVGKLDSYDFSPSGSHL
                     IGTTSSVARVGFVQLDNLHPTDFRLDDPPARVLSTQNAKIFVDHGDPLGHATIIPSPT
                     ATRDDALVLTGFLTTNLLDQGP"
     gene            9279..9932
                     /locus_tag="VMZ53_02045"
     CDS             9279..9932
                     /locus_tag="VMZ53_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27251.1"
                     /translation="MLRTTLALLLFTALGSVAHAGNNEVSITETARFLHTNSANAVTE
                     DGMVCGALGYSRRVDLPALPNLTLWATGSFGWGTTSGEMFQTMTTDVGTLSLTAGGRA
                     RYELHRLVHATARLDVGTARASLTLEDEAGHSARDAGWGPISQAGVGLDLYAVNRRNF
                     TFGMRLELGYVAMGDIDMTAKPDSESNKTLQLQMTAASLGGLNLSGSTFSASVVSQF"
     gene            9956..10744
                     /locus_tag="VMZ53_02050"
     CDS             9956..10744
                     /locus_tag="VMZ53_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUS27252.1"
                     /translation="MRRATRSPMGDAIHRVISERDVMCVFQPVVDLKSGKIFAYEALA
                     RTKAKEFEGPLALFAAAVAENMTGELGRLLRGIALEGCQGHPLFLNIHPAELNEKWVV
                     QPDDPIFNHTEDVYLEITEAVPLSHFRLCKDMLAEVRGRGVHLVVDDLGAGYSNLKYI
                     ADLQPRVVKLDRDLIAGLTKDTRLFKLVSAIVRLCSELDSRVVAEGIETLSELEAVIA
                     AGARYGQGYFLAKPAFPPPDIKWPESIKVSDEPPRKKRHDSVVD"
     gene            10725..11948
                     /locus_tag="VMZ53_02055"
     CDS             10725..11948
                     /locus_tag="VMZ53_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS27253.1"
                     /translation="MTRSSTDRSVVLAFSVLAMAACQRAPRPTTPHPPPPRPPTEPTP
                     PTGELPPPTGEQPPTQAPPKITWKHVEHATDVPNAPPAEGTYRIHLIDVGTGLAILVQ
                     GHDFNLLYDGGTNDAGEKPLRVVSYLAAALGSSGDDLCVPKNSHLPKGRRTLDHVVLS
                     HPHADHGSALDLVVHCYDVRNVWDSGRVNDAVFYRDFIEAVGKSLTATYHTAASVPED
                     HAMTVKGTSIRIERWQMFRESDVVELGAHAQFQILHAEGKKLPDPNQNSVVISVALGN
                     ARLLLVGDAESGERLDPSYPPGDVEEFLVEQHAVEIRSDILQVGHHGSKTSSRLAFLE
                     AVKPHLALVSSGPKLYGKVRLPDVEVIDALKSVGATILRTDEHDENCPVTHRIGGDSG
                     PGGCDSYMITIEPAK"
     gene            11955..12089
                     /locus_tag="VMZ53_02060"
     CDS             11955..12089
                     /locus_tag="VMZ53_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27254.1"
                     /translation="MHLLVHVLVHVDVHVHVHVHVLVLVLVLVLVLVGGRMDAGSTRR
                     "
     gene            complement(12388..14643)
                     /locus_tag="VMZ53_02065"
     CDS             complement(12388..14643)
                     /locus_tag="VMZ53_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HUS27255.1"
                     /translation="MKRRPQYLIAISLLAGCEATVDGWTKEEEPGGNDDVHQALAALP
                     NAEVLEWTPDGLPTYIVGEMMKVGAMQSDDPIASDNALRTQLGPVLAPFRLHTGDLAL
                     RRMNVDENGDRHFRYAQTFNGLPVIGGDLVVHVDVKGAVYGVNGTARGDIPADMTVSV
                     SESAARSAIAGDGRFANLTASNPRIVYLQADDGMHKAYELTVEGMRGQDPARDRVYVD
                     TQSGAIVADYPQIYFAENRKVYSLGGGTSLPGTLKRSEGQAATTDVDVNAAYDGTGDA
                     YEAYKNFWNRDSYNNAGAALISSVHYSSNYCNAYWNGTQMVYGDGSASQGCAPLARSV
                     DVTAHELTHAVTENESGLVYSGESGGINESLSDIFGTFTEAWTDGGKNGTLALSSDTW
                     LLGEDVISGGLRYMCDPAKDGASKDYWTSTAGSVDVHYSSGIGNLNFCLLTKGGTHPR
                     GKSTVNVPGIGMEKAIRIYYKAQTDILTSSSKYANLRTAMEQAATSLGYDQATKDAVS
                     CSWAAVGVGTAPTTCGGGGGGGGGGGGGTTDGTLSNGVAVTNISDATGGQKFWKLDVP
                     AGQSTLTFTIAGGTGDADLYVQSGTKPTTTVYACRPYKNGNAETCSFTPPAAGTYWVM
                     LNAYAAYSGLSLTGTYSASGGGGGTGDPYLTNGVAVSNVSGPAASATYWRIAAPAGKT
                     LTIKISGGTGDADLYTKFGARPTTASYTCRPYLNGNAETCTQTNTQAGDYYVMVRGYT
                     AFSGLSLIASY"
     gene            15098..16387
                     /locus_tag="VMZ53_02070"
     CDS             15098..16387
                     /locus_tag="VMZ53_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27256.1"
                     /translation="MRLSIVLIATGLVACSNDVDPRVIAGGGVSDGEIDGEINVAVID
                     QSTDNVIPGATVDVGGTQKTTDTKGFATFSDVEGAQTVIVKMSGYRSAVWVDVNGANV
                     TIGLQPNSSTTPPQATLSGTITGWDAITVPQGHAKAAFVITSQTTDLGDPDNDVPTGA
                     GNVCIAGTECNWTLNSRTGSVTVIAVLIDRDLHGTLTNPDDDTNKIIGWAYKTGVTVE
                     TGVNQSGLVLSQVEAGNLQTVTVDLGTPPPSLTERLTLVGIELSKDEVIQMPLFIEAT
                     DATSILAPKPGTFGGTASYRLTAIAQSTSGEMGAQSAVIRTGLASTALAAGTWLLPPT
                     SVMATRSSASFEPVAGARAHSANWRDGAGVELLEITSFNVKIKQFDVPALVALPTSGT
                     LTARVNAIGADLDPNDFSLDDDSDQIWGFAAEPASIP"
     gene            16426..16686
                     /locus_tag="VMZ53_02075"
     CDS             16426..16686
                     /locus_tag="VMZ53_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27257.1"
                     /translation="MQTNNNIETSENSAPAQVARFTELQALVAGMAADFEKFYKDGNK
                     AAGTRVRLAMQELKTFAQTVRNEVQSIKNEGKPASGDDAAEA"
     gene            complement(16759..17115)
                     /locus_tag="VMZ53_02080"
     CDS             complement(16759..17115)
                     /locus_tag="VMZ53_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS27258.1"
                     /translation="MQLAQAILFVNDAVRMQAFYEQLGLKVVDGDAKTGFVRLADSTG
                     GAVLAIHHTPAAGPGGAPRIDTWTKLCFRVEDVAAERAQLVARGVTAREIHTFGSVSF
                     CDFVDPEGNVFQLTTR"
     gene            complement(17129..18196)
                     /gene="amrS"
                     /locus_tag="VMZ53_02085"
     CDS             complement(17129..18196)
                     /gene="amrS"
                     /locus_tag="VMZ53_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HUS27259.1"
                     /translation="MEDFPAKWWHATADGRIQCDLCPRDCKLNEGQRGMCFVRQRSGD
                     RMVLTTYGRSSGFCVDPIEKKPLNHFYPGSSVLSFGTAGCNLACKFCQNWDISKSREM
                     DRLVDSASPEAIARAAKQANAASVAFTYNDPTIFSEYAMDTADACHALGIKTVAVTAG
                     YIYPEPRSELYAKIDAANVDLKAFTDEFYRKVTASSIEPVKDTLRYLVRETQVWTEIT
                     TLIIPGLNDSDAELTALAEFVAGDLSVDVPVHFSAFHPDYKMMDIPATPIATLRRARD
                     IARRAGLRYVYTGNVHDADGDRTICPSCSAAVIERDWYEILSSNLRGGACGTCGTAIA
                     GRFGESIGNFGRRRMRVVLNA"
     gene            complement(18196..18726)
                     /gene="amrA"
                     /locus_tag="VMZ53_02090"
     CDS             complement(18196..18726)
                     /gene="amrA"
                     /locus_tag="VMZ53_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HUS27260.1"
                     /translation="MSNGMTLARYAREKLREHLGGPLAVKPSGAWCDVPGATFVTLRW
                     PNGQLQGCIGTLEADRSLVDDVASNAIAAGTRDPRTRPCVLGDVDKLDVELSILSALE
                     PIGGEADIRVGIDGIVMLAQGRRATFLPVMWERLRDIPTFMAELKQKAGLPRTYAGEM
                     QLMRYTVEKHTDPAPR"
     gene            complement(18723..19517)
                     /gene="amrB"
                     /locus_tag="VMZ53_02095"
     CDS             complement(18723..19517)
                     /gene="amrB"
                     /locus_tag="VMZ53_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HUS27261.1"
                     /translation="MGVADSVRPAAVAGTFYPGDRAELQATIDGLLGVVDAPKGPCPK
                     ALIVPHAGYVYSGPIAASAFARVAPYGDRIKRVVLVGPAHRVFVDGLATPGAKKLATP
                     LGEITVDETALGGLNIPANAAAHAREHSLEVELPFLQRVVPNATIVPLCGSRAAPEAV
                     GRILETLWGGPETLIVISSDLSHYHPYAQGRAIDQMTCKAIVAREPKLVGEQACGAIG
                     INGLLWLARRKNLRVELVDLRSSGDTAGSRDEVVGYGAFALYEEAA"
     gene            complement(19533..20702)
                     /locus_tag="VMZ53_02100"
     CDS             complement(19533..20702)
                     /locus_tag="VMZ53_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HUS27262.1"
                     /translation="MNFFDDTMRRDPYRVYEQLRASSPAFHFAPADLYFLFDYASVKR
                     ALHDSAVFSSAVGASRGNSFEWLMFMDPPRHTKLRAIINRAFTSRSIAALEPRIRELA
                     VALVDEVVARGEVDLEEKLAAPLPMMVIAELLGLPLGDWPKLIGWSRAIMNLAATIMG
                     PAHESAAASADFLRADGEMQAYLGELVTARRAKPVDDLLTRLVTAEIDGEHLSQLELV
                     RFFELLLAAGTETTTNLIDNAIICLVENPDQMARLQSNVTLVPSAIEEVLRYRSPAQA
                     IFRATKQSVELHGVTIPAGKMVLPMLGAANRDPAVFPEAARFDIGRDPNPHIAFGHGI
                     HFCLGAPLSRLEARVALEELLPRIRTIELASTQWTPRAPFHVHGPASLPVRIAAR"
     gene            20738..21721
                     /locus_tag="VMZ53_02105"
     CDS             20738..21721
                     /locus_tag="VMZ53_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS27263.1"
                     /translation="MARLNDSRMIGTVLAPHLAVRATTRTFSSPHAAHGIALFVGLEH
                     DVAITTPGGHARGRAVIVAPDVAHSVSSPGPTIGICFDPEALPRVTGFARAIGSARAA
                     GSARSDRFARAAASARADGFARADGSASADLVVGPYGGQARSQRETNEPRLVACPGDG
                     QARSRRETDDASLVACPGGGQANVLDGRLGRLVLGAALAHRSELGRGDVLAGVGGEIA
                     AAIGSNAGAAEARLDRRVARVVEALRDPAAELPRLSISDAHLQELFARDVGVPIRSYR
                     LWHRLMRALVLFAHGDATTAAHAAGFADLAHFSRTCRRFLGYSPTTLRNGV"
BASE COUNT         3778 a         7316 c         7018 g         3682 t
ORIGIN      
        1 cggcgcgtca gcggcggccc cattcatcgt cgacggaggg tacactgcca gcgatggccg
       61 agatcacgat agccgtcgtg ccgtcggcca cgcccggcga cgacagcggc ttcgtcctcg
      121 atgggctgtg cgtcgcgctc acgaagctgc tcgacgtgcc cgtatccggc atcaactcgc
      181 gcagctatca agatctcgcg gcggagctgg agaagggccg cgtcgactac gcgtggatgt
      241 cgccgacgct gatgctgctg actgcggagc acattcagct gcggccgctg ctgtcggcgg
      301 ttcgcaacgg cagcaccgaa tattgctcgg cgctgtttgt cgacgacgcg atgccgatcg
      361 acgagatcga cgacctccac aactccacgg tcgcgtgggt cgattcgacg tccgcgtcgg
      421 gctacctgtt cccgcgactg atgctcgccg cgcagggcaa agaccccgcg cacctctttc
      481 gcaaggagct ctttctccgc tcgcatgcgg aagtggtgcg cgctgtcctc gacggcaagg
      541 ccgacgtcgg cgcgacgtac gcgcaacgcc ccgtggaggg cgagcctgtc acgcgtgccg
      601 gctggctgca cgttgccccg actcgcagcg tgcgcgtcct cgagtattcg cagccgattc
      661 cgaacgacat gatcgtcggc cacggcctga tcgaaaaggc gcaccacacc gacttcgtca
      721 acgcgatctt gacgctctcg aacctcgaga atggcaagcg cctcctctat cgcgcctttc
      781 acgcagagca gttcaaggcc acaccgcgca acgcgctgag gccgctggag atgctgctgc
      841 agactgcacg gtcacacggc ctcttctcgc acctctagcg agaggccatg aagatatcgg
      901 tcccgcgcgc gaagtagatc gttgtctcgt cgggagacag ccacgggtca ctctccgtgc
      961 tggcggtgtt cagctcggtg atcggcgccg cggtgcccca ggtcgccgac gcatccgcac
     1021 gacgcgccat ccacaaatca tcgttgccgc tcacgcgcgt gctcgtccac acgaggaacg
     1081 acttcgtgcc gttcacccac ggctcgtgat ctggcgacgt cgtgttgagc tcggtgattt
     1141 gtgctgccgt cgtgaacgcg gctgtcgtcg ttgtccgccg tgcgacgtac aggttgtcat
     1201 tccccggcgc atcactcgcg aagtacagct cgagaccgtc ggcactgagg aacgggcccg
     1261 catcatcgcc ggtcgtcgag atcgcggtga gctcttgcgc agcgctccag gcagcggtgc
     1321 gcgacgggcg cgtcgccatg aagatgtcct tcattccctt ggagccggcc gcagtccaga
     1381 gcagcgtcag cccgtcacgc gacagcgcgg gcgtcgcgtc gtcaccggcg gtgttgagcg
     1441 tcgagaggtt cgtgacgccc gtccagcccg acgtgagcga gggacgcgac gccgtccaca
     1501 tatcacccgc gccgacgcca cccgcgcgat ccgaattgaa gacaatctcc agctgatcgc
     1561 ccgtcagcga cggatcatct tcgctcgcag cggtgttcaa accgggaacg ggcatcgccg
     1621 tcccaaacgg gcccatcggc ggcagcgcat cgggcggtgg cgcgtcgatc gatgaatcgg
     1681 tgctcacgac catggcgtcg tcgcgcgtgt tgcccgggtc gaacccgaat cgaccgcacg
     1741 cagatgctgc gagagcggcg gcaagcccta ggcggaacac cagtcgaatg tagactgcgg
     1801 gtatggcgcg ccgccatcgc gagagacacc gcagcggacg gaccgggtgg ctgcgagcag
     1861 ccgtcctcgg cagcaatgac gcgatcgtgt cgaccgccgg cctgatgatg ggcgttgcgg
     1921 cgaccgacgc ttcgacgcag accatcctca tcgccgggat cgcgggcctg tgcgcggggt
     1981 cgatgtcgat ggcagcaggc gagttcgtct ccgtgagctc gcagcgcgat gccgagcaag
     2041 cagacatcgc gaaggagacg cgagagctcg aggaggaacc ggaagctgag ctgaaggagc
     2101 tcgcgggcat ctatcacagc cgcggtctcg acaaggcgct cgccatgcag gtcgcgaagc
     2161 agctcagcgc gcatgatcgc ctcggcgctc atcttcgtga cgagctcggc atcgagccga
     2221 acaaccgcgc gcgtcctctg caggcggcgt ggatctcggc cgttagcttc gcgctgtttg
     2281 cgctcgttcc gatcatcgcg cttctgatcg ctcccaaaaa ctttcggatg gagacgatcg
     2341 ccatcatcac cttggtaagc ctcgccgcac tcggcgccct cggtggaaag ctcggtggag
     2401 cgccactcgt gcgagccgcc ctgcgcgtca ctttcggcgg tgcgctcgcc ctcgcagtga
     2461 cctacggaat cggtcgcctt ctcggcctga cgttgtagag ttgccggcca tgtgccggaa
     2521 cattcgcgtc cttcactact tcaagccgcc gaccacccac gaagaagtgc acgcagcagc
     2581 gctgcagtac gtgcgcaagg tcagcggcat gcgcgacccg agcgacgaag cggcgaaggc
     2641 cttcgagaaa gccgtggagc agatcgcggc cgcgacgtcg aagctgctcg gcgtgcttcc
     2701 gaagaagggc gagccacgca cgcgcgaggg cgagaagctc aaggcgaagg cacgctggga
     2761 acgtcgcgag aagcagatcc gctcagcgga gtagcagcac gacgacgacg attccaatcg
     2821 cgatgatcgc gagcgcgacg acaaacgatc ggctcgaaat atctccaccg gtttcggtcc
     2881 gcccgcgctc caccatctga cgcgtctcgg aagcttgtcc gggacgttct ggtccaaggt
     2941 ccggcagggt caccgcagga gtgtgcacga ggggcgcggc gcgcgcgaat gactctgaca
     3001 gcgccaagcg tttccaatcg ttaggctggc tcgggccctg caaccctctt taccgtgcgg
     3061 taccacgctc ttgcagcgga ctacgatgga acgctcgcgc atcacggcct catcgatgat
     3121 ctgacttggg cgtcgctcaa gcagcttcgc gaatcgggcc gcaagctgat catggtgacg
     3181 ggacgcgagc tcgacgagct gctcgcactg ttgccgcacc ccgagatgtt cgatcgcatc
     3241 gttgccgaga acggcgcggt gttgtacagc ccggcgtcta agaaggtcac gttgctcgcg
     3301 ccgccgcctc cgccggagtt tggcgacgag ctgaagaagc gcggggcgga gcgcgtcgcg
     3361 gtcggccgcg tgatcgttgc gacatgggag ccgcaccaag acacggtgct ccacgtcatc
     3421 cacgacatgg gcctcgagct ccaggtgatc ttcaacacgg gcgccgtcat gatcctgccg
     3481 accggggtca acaaggcgac gggcctcgag gctgcgctgc acgatctcgg gctctcgaag
     3541 cacaacgtcg tcgcagtggg cgatgcggag aacgaccacg cgctgctgac gcgtgcggag
     3601 ctcggcgtcg cggtcgcaaa cgcgctgccg tctctgaagc agcacgcgga tctcgtgacg
     3661 cctggcgatc acggtacggg cgtctcgcaa gtgatcgatc gcttgatcgc gaacgacctc
     3721 gcggatatct cgccgccgcg gcatcggatc aaagtagggc aggcgaacgg ccagcacgtc
     3781 acgatcgatc cgtacgagac gaacatcatg atcgccggca cgtcgggcag cggcaagtcg
     3841 acgctgacga ctgcgatcct cgagcgcctc tgcgaacgcg gctatcagta cgtaatcctc
     3901 gatcccgagg gtgactacac atcgatcgaa agcgcagtgg tcctcggtgc tccgcatcgc
     3961 gcgccgctcg tcgacgaagt catcgatgtc ctgcgcgatt cgtcgcgcaa cgcggtcgtc
     4021 aacatgctcg gcatcgccgt cgaccatcga ccggagttct tcgcgaagct gctccccgcg
     4081 ctggcggagc tgcgcatgcg aacgggcagg ccgcactggc ttgtcgtcga cgaagcgcac
     4141 catctgctcc cgtcgtcgtg gcagcccgtc gccgagctcg cgctacgccc gcacggcacg
     4201 ctctacgtca cggtgcatcc gggaagcgtc gcgcagccgg tgatcgatac gatcaatacg
     4261 gtgttcgtcg tcggtgatca tccagacaag accgtgaagg aattctggaa cgtcgcgcgt
     4321 atcaagaagc cgccacatgt cgcgagcgag aagctaggcc caggccacgc gctctattgg
     4381 gagacgaaga cgaacctcgc tgccgtgatc gagacagagc catcgacgac cgagcgcacg
     4441 cggcactcgc gcaagtacgt cgagggcaac ctcggctcgc atagcttcgt gttccgcggc
     4501 cccgacaaga agctgaacct caaagcgagc aacttgatgc tgttcttgct gctcgccgaa
     4561 ggtgtcgacg acgacacgtg gctccatcat ctccgcaacg gcgagtactc gcagtggttc
     4621 cgcacgaacg tgaaggaccc cgacctcgcg caagcggccg aagcggcaga gaaggacgca
     4681 tcgatctcgg cacaggcaag ccgcgagctc atccgcgccg agatcgagaa gcgctacacg
     4741 ctgccggccg acgaaccgag cggcgtcatc gatgactgag gcgtagcctt cgccggcggc
     4801 tcgtcgacct tctcgacgcg accatccgcg tgacgtgcga accggccttc ggtcttgccg
     4861 tgcgtcggat cgcctttggg ccacggccag ccaccgaact gcgtgcgccg gaagtccaag
     4921 aacgcctgct ggatctctgc ggccgtgttc atcacgaacg ggccgtgctg gacgacgggc
     4981 tcaccgatcg gcttgccttg cagcaccagg atctcgacgc catctttccc agcgacgagc
     5041 tgcacctccg cctcgggacg aagccgtgca cctgcgtgct tcacgagcga cttctcgccg
     5101 agcgtcagac gatcgccggc gtagaagtag agcgtgcggt tcgtgccggc cttggcggca
     5161 ggcagcgtcc actcggcgtt cggcgccatc ttgatcgacc agatcgcgac gtccgcatcg
     5221 gcgcgtgatg cccacgagtc cggcggagcc ggcagcggct tcgtctcgcc gagcgtgccc
     5281 gccacgacga cgacctcggt cgtacggcct tgcgagtcgc gcgcgacatg acgcgggatc
     5341 gtgtgatccc acagcatcga gaagtagggg ttcaccatct tcgaggcgcg cggcaggttc
     5401 agccagatct ggaacagctc gaccggattg ccgcccgact tgtcgagcag cgggaacatc
     5461 tccgagtgga cgatgccctt acccgccgtg agccattgca cgtcgccacg gccgaagcgc
     5521 gcggcagcac cgagcgaatc ggagtgatcg atgaggccac gacggacgat cgtcacggtc
     5581 tcgaaaccgc gatgcggatg acccgggaag cccggcaccg tgtcgccgtg atacatgcgc
     5641 cagccgtctt tgccggcgaa gtcctggccg atgtcacgac cgtcgagcga ggcagcggga
     5701 ccgaggtcgt cgttgcccgc ggggtacgcg tcgtcgtgat gcacacagaa caggaatgga
     5761 tcgaacgtct gccactgaaa gcccaacggc aagagctcga gaaccgggtc cgacttcata
     5821 gtcctccatc agtaagcctc cggtacgccc gcagcaatct cgccgattga gcagcactgt
     5881 tccgttaccg cgacattggt gtcagagctt tgctgccgac tggcaagttg cagcacgcaa
     5941 cgcaacgaga tcgctgaaga gcgcgcggca catccgttgc cctagccgcg ctgcatgcga
     6001 ttgctctatc tcgcgccgat gtttgcgctc gtggcctgtg ggggcgacca gctcgacgat
     6061 ggttacggct acggcgcgcc cgacgccggc tactcgtccg acgctggctt cgtcgggtgc
     6121 ctgtcgtcga acgaatgcgc acccggctat tactgcaacg acttcgggcg ttgcgagaag
     6181 caggcgaccg gttccggcga cggcggtatg ccgactccgc ctcccgagaa ggagtacgag
     6241 ttcgcaccgc cgacgtcgtc gcagcggttc gtctatgtcg cgatgactgc gcaggacgag
     6301 ctcgcacgga tcgacggtgc aacgctcgcc gtgaaatcga cgaaggtcgg caagtcgccg
     6361 aaggtggtcg cgacgattcc aggcaccgac ggtgctgtcg tcctcgattc gttcaacggc
     6421 accgcgacgg tcgttcgtcc cgtcggcgat accgactcga cgcgcgtgct cggcacgctg
     6481 cagcggctca atcgcctcga catcgatccc accggccggt tcgccgtgat ctggttcgat
     6541 ctcgcgaaag cgatcaagga gggcaacacg ttctccgtcg gcagcttcca ggacgtcacg
     6601 gtcgtgcgcc tggcggcggg caacgagaag gcggtgaacc tcaccgtcgg attccggcct
     6661 cgcgaagtgc agttcgacgc ggcaggcaac cgcgcctacg tgatcacgca agacggcgtc
     6721 tccgtgatcg atctcggata cgccacggat cacaccgcga cgatcatccc gccgatcgcg
     6781 gtcgccgatc cgagcatctc gccggatgac ctcgaggtcg acgtcgtatc gacgggcgag
     6841 tgggcggccg ttcgtcagac gaacagcgca acgctgcgcg tggtcaacgt cggctcgagc
     6901 cccggtacgg cgtactccgt cacgctcgcg tcgccggcga ccgatatcga cctcgcaccc
     6961 aatggcgcgc ggctctacgc ggttcagcgc gccgcgaaga agctctctgt cgtcgacatc
     7021 cccggcgatg cgcagaatcc gagcggcgtc gagacgatcg acctgacgaa cgcgacggtc
     7081 ggctcgctga cgctgtcgct cgacggcaag cgcggcctga tgttcacgaa tgcaacctcc
     7141 gacgagcggg tcacgctgat caagctcgac caacccggct atccgcagtc gacgtggccg
     7201 atcaagaaat cggtccgcgc ggtggcagtg tcgccatcgg gtgacacggc gctgctcatc
     7261 aacgcgaaga tggcgggcga tccggcgacg gcctcgacgt tcgatgactt cgtcgacaag
     7321 agctacggct acacgttgct cgatctcgcg agcggatttg gcaagctgca gatcacgccg
     7381 gtggatcccg gtgcgttcac ctacgcaccc gacggcacga aggtctacgt cgcgctcgac
     7441 ggcggcgatg caccgaccgc gacgcgcgcc atccaagtgc tcaccacgca gaccggtgtc
     7501 gtccagacga agacgctcgg ctccccgccg tcgtcggtcg gcatcctgcc gggcgcggcg
     7561 caggcgttcg tcaatcaacg tcatccactc ggtcgcgtct cgttcgttga tctcatcaac
     7621 gacgcgcagc gcaccgtcac cggcttcgat ctcaacagcc acatcgtgaa ctgaggaatt
     7681 gccatgaaga cgatgctcct cgcgctcgcc gccctcgcgg cactgccggc ctgctggctc
     7741 ggcgaagatg aacgtccgat cgtctgggat cgcgagcgtc acgtgctcgg ccccatcccg
     7801 atgaagacgc agatcgcgta cgtcgattcg gcgctcgatc gcgtcacgct gctcgacctc
     7861 gcgaatgcgc agcccgaaat ctcgacgcag aagatcggcc gccgagccgt cgtcgcggtg
     7921 ccgagcccgg atcgccatct cctgttcgtg atcacccgcg gtgaagaagc gattcacgaa
     7981 ggtgagatcg atcaggcgcc gatgttctgg gtcatcgaca cgtcgaatcg tacgccgccg
     8041 accgcctacg agatcggctc tccgttcgat cgcgtggcgg tcgcgcccga caacagcatg
     8101 gtcgtttgct acttctccgc ggccggcccg gacgaagcgg gcttcttccg aaatccgaac
     8161 gagctcgcga tcattcacct gaccgaggct ccgagcgact cgaacccgac gctgcggacg
     8221 atccgctcgt tcggctcgac gcccgaaggc gtgaccctct cgccgccgat gatcgttccc
     8281 ggtgctgccg atggcgagcc gcgaacgttc gcgtacgtgc tgtcgtcgaa caacctgacg
     8341 ctcgtcgata tctcgcatcc cgatcgtcgc gagatgtcga ttcgcctcga tctcggcggc
     8401 agtgccgtcc tcccgaagga ggtcgtgttc gcgccgcata ccgctaccgc ctacgtccga
     8461 agcgacaacg cgcgcgacgt cctgcaggtc ttgctcgagg ccgtgccgcc gacggaggcg
     8521 aacgccaatg acttccgccc cgtgctcgcc gagctcggcg ccggtggtgg acccacggac
     8581 atcgcggtct acgacgatcc gagtggccgg cgctacgtgc tcgccgcgac gccgaacacg
     8641 aaagaggtcg tcgtgatcga cgccgatact gcgcagttcc gttcggtgcc cgtccccgac
     8701 gcgatcgatc gaattctcct gttccccgtc ggagtcgaca cgccgccgac gaaggcgctg
     8761 ttcgcgtcgg tcgccgcgaa gagcacgaag gtcaatgtcc tcgacctcgt ccacatcgag
     8821 gatccgctca cgcaggcgag catgcgcacg atcactctcg acaagccggt gcgcgacgtc
     8881 gtccccgtgc ccggccgcga cctcgcgatg ctcgtccacg atgatgcgcg caccgtgctc
     8941 ggcctgctcg acatggatac cgagtcgacg tcgccgctgc tcggcgtcgg caagctcgat
     9001 tcgtacgact tctcgccgag cggctcgcac ttgatcggca cgacatcttc ggtggcgcgc
     9061 gtcggcttcg tgcagctcga caatctgcat ccgacggact tccgcctcga cgatccgccg
     9121 gcgcgcgtgc tctcgacgca gaacgcgaag atcttcgtcg accacggcga tccgctcggt
     9181 cacgcgacga tcattccgtc gcccaccgcg acgcgcgacg acgcgctcgt gctcaccggc
     9241 ttcctcacca ccaacctcct agaccagggg ccctgactat gttgcgcacc acgctcgctc
     9301 tcctcctgtt cacggcgctc ggttcggtcg cgcacgccgg caacaatgaa gtctcgatca
     9361 ccgagaccgc gcgcttcctc cacacgaatt cggcgaacgc cgtcaccgag gacggcatgg
     9421 tgtgcggtgc gctcggctac tcgcgtcgcg tcgacctgcc ggccctgccg aacctcacgc
     9481 tgtgggcgac cggcagcttc ggctggggca cgaccagcgg cgagatgttc cagacgatga
     9541 cgaccgacgt cggcacgctg tccctcaccg ccggcgggcg agcgcgctac gagcttcacc
     9601 ggctcgtcca cgcgacggcg cggctcgatg tcggcactgc acgcgcatct ctcaccctcg
     9661 aggatgaggc aggtcacagc gcgcgcgacg ccggctgggg accgatctcg caggcgggtg
     9721 tcggcctcga cctctacgcg gtcaatcggc gaaatttcac gttcgggatg cgcctcgagc
     9781 tcggctacgt cgcgatgggc gacatcgaca tgacggcgaa gcccgactcg gagagcaaca
     9841 aaacgttgca gctgcaaatg accgccgcca gcctgggcgg gctgaatctc agcgggagca
     9901 cgttctccgc ttcggttgtg agccagttct gaaggaagta tgatgtgctc ttcacgtgag
     9961 aagggctact cgatcgccga tgggcgacgc tatccaccgc gtcatctccg aacgcgatgt
    10021 gatgtgcgtg ttccaaccgg tcgttgatct gaaatccggc aagatcttcg cgtacgaggc
    10081 gctcgcgcgc acgaaggcga aggagttcga aggaccgctc gcattgttcg ctgcggccgt
    10141 cgccgagaac atgacgggcg agctggggcg cttgctccgc ggcatcgcgc tcgaaggctg
    10201 ccaaggccat ccgctgtttc tcaacattca tccggcggag ctcaacgaga agtgggtcgt
    10261 ccaacccgac gatccgatct tcaatcacac cgaggacgtc tacctcgaga tcaccgaggc
    10321 agtgccgctg tcgcacttcc gcctctgcaa agacatgctc gccgaggttc gcggccgcgg
    10381 tgtccacctc gtcgtcgacg atctcggcgc cggctactcg aacctcaagt acatcgccga
    10441 tctccagccg cgcgtcgtga agctcgaccg cgatctgatc gcaggcctca cgaaggacac
    10501 gcgcctgttc aagctcgtgt ccgcaatcgt gcgcctgtgc agcgagctcg acagccgcgt
    10561 cgtcgcggaa ggtatcgaga cgctcagcga gctcgaagcg gtgatcgccg cgggcgcgcg
    10621 ctacggccag ggatacttcc tggcgaagcc tgccttcccg ccgcccgaca tcaagtggcc
    10681 cgaatcgatc aaggtctccg acgagccgcc gcgcaagaag cgacatgact cggtcgtcga
    10741 ctgaccgctc cgtcgtcctc gcgttctcgg tgcttgccat ggccgcgtgt cagcgcgcgc
    10801 cgcgtccgac gacgccacac ccgccgccac cccgcccgcc gaccgagcca acgccaccga
    10861 cgggagagct ccctccgccg acgggcgagc agccgccgac gcaagcgccg ccgaagatca
    10921 cgtggaagca cgtcgagcac gcgacggacg ttcccaatgc cccgcccgcc gagggcacgt
    10981 atcgaatcca tctgatcgac gtcggcacgg gcctcgcgat cctcgtccaa ggccacgact
    11041 tcaacctcct ctacgacggc ggcacgaacg atgcgggcga aaagccgctg cgcgtcgtct
    11101 cgtatctcgc cgcggcgctc ggctcgtcgg gtgacgacct ctgcgtgcca aagaacagcc
    11161 acctgccgaa aggccggcgc actctcgatc atgtcgtgct ctcgcacccg catgccgacc
    11221 acggctccgc gctcgatctc gtcgtccact gctacgacgt ccgaaacgtc tgggactcgg
    11281 gccgcgtcaa cgatgcggtc ttctatcgcg acttcatcga ggccgtcggc aagtcactga
    11341 ccgcgaccta ccacacggcc gcaagcgtgc ccgaagacca cgcgatgacc gtcaaaggca
    11401 cgtcgattcg catcgagcgg tggcagatgt tccgcgagtc ggacgtcgtc gagctcggcg
    11461 cgcacgcgca gttccaaatc ctccacgccg aaggcaagaa gctccccgat ccgaaccaga
    11521 actccgtcgt catctcggtg gcactcggca acgcgcgcct tctcctcgtc ggtgatgctg
    11581 aatccggcga acgcctcgat ccctcatacc cgccgggcga cgtcgaagag ttcctcgtcg
    11641 agcaacacgc cgtcgaaatc cgctccgaca tcctgcaagt cggtcaccac ggcagcaaga
    11701 cctcgagtcg cctcgccttc ctcgaagcag tgaaaccgca cctcgcgctc gtctcgagtg
    11761 ggccgaagct ctacggcaag gtgcgcctgc ccgacgtcga agtgatcgac gcgctgaaga
    11821 gcgtgggcgc gacaatcttg cgcaccgacg agcacgacga aaactgcccg gtcacccatc
    11881 gcatcggtgg tgactccggc ccaggcggct gcgactcgta catgatcacg atcgaacccg
    11941 cgaagtaaac actcgtgcac ctcctcgtgc acgtcctcgt ccacgtcgac gtccacgtcc
    12001 acgtccacgt ccacgtgctc gtgctcgtgc tcgtgctcgt gctcgtgctc gtcggcggtc
    12061 gcatggacgc cggttcgact cggcggtgaa gttcgcgcga gcaggggaaa gcggcacacg
    12121 cagagaccga cagcgtggac gtgcacgtgc acgtgacacg tggacgtgga cgtggacgtg
    12181 acacgtggac gtggacgtgg acgtggacgt gcacgtggac gtgacacgtg gacgtggacg
    12241 tgacacgtgg acgtgggacg tgggacgtgg gccgtcggaa gtgcacgctc gcgacgccga
    12301 aaaaggcaac ggggctcggt ccatcggggg ttcctccttt gcgaacccga tcgaccgaac
    12361 cccgtcgggg ttcacgtatg cgcgcgctca gtaactcgcg atgagggaga gtcccgagaa
    12421 ggccgtgtag ccccggacca tcacgtagta atcaccagcc tgcgtgttgg tctgcgtaca
    12481 ggtttccgcg ttgccgttga ggtacggacg gcacgtgtag ctcgccgttg tcggtcgcgc
    12541 gccgaacttc gtgtagaggt ccgcgtcacc cgtgccgccg ctgatcttga tcgtcagcgt
    12601 cttgcctgcg ggtgctgcga tgcgccagta cgttgcgctc gctgcgggac ccgagacgtt
    12661 cgagaccgcg acgccgttcg tgaggtacgg gtcgcccgtg ccaccaccac cgccgctcgc
    12721 cgagtacgta cccgtgagcg agaggccaga gtaggcggcg tacgcgttga gcatcaccca
    12781 gtacgtgcca gcggccggcg gcgtgaacga gcacgtctcg gcgttgccgt tcttgtacgg
    12841 acggcaggcg tagacagtcg tcgtcggctt cgtgccggat tggacgtaga ggtcggcgtc
    12901 gcccgtgccg cccgcgatcg tgaacgtcag cgtcgattgg cccgcgggaa cgtcgagctt
    12961 ccagaacttc tggccgcccg tcgcatcgct gatgttcgtg actgcaacgc cgttggagag
    13021 cgtgccgtcg gtcgtgccgc cgccgccacc gccgccacca ccgccgccac cgccgcacgt
    13081 cgtcggagcg gtgccgacgc caaccgcggc ccacgagcag ctcacggcgt ccttcgtggc
    13141 ttggtcgtaa ccgagcgacg tcgcggcctg ctccatcgcc gtgcggaggt ttgcgtactt
    13201 cgacgacgac gtgaggatgt cggtctgcgc cttgtagtag atgcggatcg ccttctccat
    13261 gccgatgccc ggcacgttga cggtcgactt gccgcgcggg tgcgtgccgc ccttcgtgag
    13321 gaggcagaag ttgaggttac cgatgcccga cgagtagtgg acgtcgacgg agccggccgt
    13381 gctcgtccag tagtccttcg acgcgccgtc cttcgccgga tcgcacatgt agcgcaggcc
    13441 gcccgagatg acgtcctcgc cgaggagcca cgtgtcgctc gagagcgcga gcgtgccgtt
    13501 cttgccgccg tcggtccacg cttccgtgaa cgtgccgaag atgtcggaca gcgactcgtt
    13561 gatgccgccc gattcgccgg agtagacgag acccgactcg ttctcggtca ccgcgtgcgt
    13621 cagctcgtga gccgtgacgt cgacggagcg cgcgagcgga gcacaaccct gcgacgcgct
    13681 gccatcgccg tacaccatct gcgtgccgtt ccagtacgcg ttgcagtagt tcgacgagta
    13741 gtggacggag gagatgagcg ccgcgcccgc gttgttgtac gagtcgcgat tccagaagtt
    13801 cttgtacgct tcgtacgcgt cgccggtgcc gtcgtatgcg gcgttgacgt cgacgtcggt
    13861 cgtcgcggcc tgaccttcgc tgcgcttcag cgtgccgggc agcgacgtgc caccgccgag
    13921 cgagtacacc ttgcggttct cggcgaagta gatctgcgga tagtcggcga cgatcgcgcc
    13981 gctctgcgta tcgacataga cgcggtcgcg cgctgggtcc tgaccgcgca tgccttcgac
    14041 cgtcagctcg tatgccttgt gcataccgtc atcggcttgc agatagacga tgcgcgggtt
    14101 gctcgcggtg aggtttgcga accggccgtc gcctgcgatc gcggaccggg ccgcggattc
    14161 gctgaccgag acggtcatgt cggccgggat gtcgccgcgc gccgtgccgt tgacgccgta
    14221 gaccgcaccc ttcacatcga cgtgcacgac gaggtcgccg ccgatgacgg gcagaccgtt
    14281 gaacgtctgc gcgtagcgga agtggcggtc gccgttctca tcaacgttca tgcgccgcag
    14341 cgcgaggtcg cccgtgtgca ggcggaacgg cgcgagcacg ggaccgagct gcgtacggag
    14401 cgcgttgtcc gatgcgatcg gatcatccga ctgcatcgcg cccaccttca tcatctcgcc
    14461 gacgatgtat gtggggagtc cgtcgggagt ccactcgagg acttccgcgt tgggtagtgc
    14521 tgcgagtgct tgatgcacat cgtcgttccc gcccggctcc tcctccttcg tccaaccatc
    14581 gacagtggct tcgcagccag caagcagcga gatcgcgatc aggtattgag gacggcgctt
    14641 catgcggaag ctgatgtgct tgggtgatgc cagcaccgct tgcgcatgtt gtgcagaaca
    14701 ggtcggcagc gctgtcagtc agcgcgaggc agcgcatcta acgcagttgc gcggacactt
    14761 tgaggtcaaa gcttgtatcc caagcgacat tcgcagtcgg ccgatgtgcc cccttgtgat
    14821 cctgatgtgg gagaaaatcc gcgatgccag gtagtcgcgg cggatagcag gtgtcatccc
    14881 cgtttacgcg atgacagctc acttctgcaa tcgagttgca attagctgcg ccacgccgtc
    14941 acggcggata tggctcccgt cctgatctgc ctctgcggcg gtgtgtcctc gaggagtcgg
    15001 tcatcccaca tctgccgcga tgcgttgttg acgcgtcgtc aacaagccgt accgcagtga
    15061 tttcgtgtgc gcgccgcgtc agtcgtatga tcgcatcatg cgcttatcca ttgtcttgat
    15121 cgccacgggg ttggtcgcgt gcagcaacga cgtcgatccg cgcgtcatcg cgggcggcgg
    15181 cgtctccgac ggagaaatcg acggcgagat caacgtcgcg gtgatcgacc aatcgaccga
    15241 caacgtgatc cccggcgcga ccgtcgacgt cggcggtacg cagaagacga ccgacacgaa
    15301 aggcttcgcg acattcagcg atgtcgaagg cgcgcagacc gtcatcgtga agatgagcgg
    15361 ctaccgcagc gcggtatggg tcgacgtcaa tggagcgaac gtgacgatcg gtcttcagcc
    15421 gaacagctcg acgacgccgc cgcaagcaac gctgtcggga acgatcacgg gctgggacgc
    15481 gatcacggtt ccgcaaggcc acgcgaaggc cgcgttcgtg atcacgtcgc aaacgaccga
    15541 cctcggcgat ccagacaacg acgtcccgac aggcgcgggc aatgtctgta tcgcggggac
    15601 ggagtgcaac tggacgctga attcgcggac cggctcggtg actgtcatcg cggtcctcat
    15661 cgatcgcgat ctccacggca cgctgacgaa cccggacgac gacacgaaca agatcatcgg
    15721 ctgggcgtac aagaccggcg tcacggtcga aacaggcgtc aaccaatcgg gcctcgtgct
    15781 cagccaagtg gaagccggca acctccagac cgtgactgtc gatctgggca caccgccgcc
    15841 gagcctgacc gagaggctga cgttggtcgg catcgagctc agcaaggacg aggtcatcca
    15901 gatgccgctc ttcatcgagg cgaccgacgc gacctccatc ctcgcgccga agccgggcac
    15961 gttcggcggc accgccagct accggctgac cgccatcgcg cagtcgacct ccggcgagat
    16021 gggcgctcaa tccgccgtca tccgtaccgg cctcgccagc accgcgctcg cggccggaac
    16081 atggctcctt ccaccgacga gcgtgatggc cactcggtca tctgcgagct tcgaaccggt
    16141 cgcgggcgcc cgcgcgcact ccgcgaattg gcgcgatgga gcgggggtcg agctgctcga
    16201 gatcacctcg ttcaacgtca aaattaagca atttgacgtg ccggccctgg tcgcgctccc
    16261 gacttctggc acgctcactg cgcgggtgaa cgccattggg gctgacctag atcccaatga
    16321 tttcagcctt gacgacgatt cagaccaaat ttggggcttc gcggccgagc cggccagcat
    16381 cccgtagttg ccgttgtcag ggtggtgggg caaggtaggt cccccatgca gaccaacaac
    16441 aacatcgaaa ccagcgaaaa ttcggccccg gcgcaggtcg cccgcttcac ggagctccaa
    16501 gcgctcgttg ccggcatggc cgccgacttc gagaagttct acaaggacgg caacaaggcc
    16561 gccggcaccc gcgtccgcct cgcgatgcag gagctgaaga ccttcgcgca gaccgtccgc
    16621 aacgaagtgc agtcgatcaa gaacgagggc aagccggcct cgggtgacga cgccgccgag
    16681 gcctagtcaa aacaccggct ggccgcggct agccccttat cttcggccgc ctctcggcgg
    16741 tcgttgcatt ttcggcccct agcgcgtcgt cagctggaag acgttgccct cgggatccac
    16801 gaagtcgcag aaggagacgc tcccgaacgt gtggatctcg cgtgcagtca ccccgcgcgc
    16861 gaccagctgc gcccgttccg ccgccacgtc ctccacgcgg aagcagagct tcgtccacgt
    16921 gtcgatgcga ggcgctccgc ccggaccggc cgcgggagtg tggtgaatcg cgagcaccgc
    16981 gccgccggtc gaatcggcca gccgcacaaa tccggtcttg gcgtcgccat ctacgacctt
    17041 caggccgagc tgctcgtaga aggcctgcat ccggaccgcg tcgttcacga acaagatcgc
    17101 ctgcgccagc tgcatgcgca gagcatagct aggcgttgag cacgacgcgc atgcggcgac
    17161 ggccgaagtt gccgatgctc tcgccgaatc gaccggcgat cgcagtgccg cacgtcccgc
    17221 aggcgccgcc gcgcagattc gacgacagga tctcgtacca gtcgcgctcg atcacggccg
    17281 cgctgcacga ggggcagatc gtgcgatcgc cgtcggcgtc gtgcacgttg cccgtgtaca
    17341 cgtagcgcag gcccgcgcgg cgagcgatgt cgcgtgcgcg tcgcagcgtc gcgatcggcg
    17401 tcgccgggat gtccatcatc ttgtagtcgg gatggaacgc gctgaagtgc accggcacgt
    17461 cgacgctgag gtcgccagcg acgaactccg cgagcgcggt gagctcggcg tcgctgtcgt
    17521 tgagcccagg aatgatgagc gtcgtgatct cggtccagac ctgcgtctcg cgcacgaggt
    17581 agcgcagcgt gtctttgacg ggctcgatgc tcgacgcggt gaccttgcga tagaactcgt
    17641 cggtgaatgc tttgaggtcg acgttcgcag cgtcgatctt ggcgtacagc tcgctgcgcg
    17701 gctcgggata gatgtagccg gcagtcaccg cgaccgtctt gatcccgagt gcgtgacacg
    17761 catcggcggt gtccatcgcg tactcgctga agatcgtcgg atcgttgtac gtgaacgcga
    17821 cgctcgcggc gttcgcttgt ttcgctgcgc gtgcgatcgc ctcggggctc gctgaatcga
    17881 cgaggcgatc catctcgcgc gacttcgaga tgtcccagtt ctggcagaac ttacaggcga
    17941 ggttgcagcc ggccgtgccg aacgacagca cgctcgagcc tggataaaaa tgattgagcg
    18001 gcttcttctc gatcggatcg acgcaaaagc ccgacgagcg gccgtaggtc gtgagcacca
    18061 tgcgatcgcc cgaccgttgg cgcacgaagc acatgccgcg ttgcccttcg ttgagcttgc
    18121 agtcgcgtgg gcagaggtcg cactggatgc ggccgtccgc ggtggcgtgc caccacttgg
    18181 cgggaaagtc ttccattagc gaggcgctgg atcggtgtgc ttctcgacgg tgtaccgcat
    18241 gagctgcatc tcgccagcat acgtgcgagg cagcccggcc ttctgcttga gctcggccat
    18301 gaacgtcggg atatcgcgga ggcgttccca catgacgggc agaaacgtcg cgcgtcggcc
    18361 ctgtgcgagc atcacgatgc cgtcgatacc gacgcgaatg tccgcctcgc cgccgatcgg
    18421 ctcgagcgcc gataggatcg agagctcgac gtcgagcttg tcgacatcgc cgagaacgca
    18481 tggacgcgtg cgtggatcgc gcgtgcccgc agcgatcgca ttcgacgcga catcatcgac
    18541 gaggctgcga tcagcttcga gcgtgccgat gcagccttgc agctgtccat tgggccagcg
    18601 cagcgtcacg aacgtcgcgc cgggtacgtc acaccacgcg ccggacggct tcacggcgag
    18661 cggaccgccg aggtgctcgc gcagcttttc gcgcgcatag cgggcgagag tcataccgtt
    18721 gctcatgcgg cctcctcgta gagcgcgaag gccccgtagc cgacgacttc atcgcgcgag
    18781 ccggcggtgt ctcccgagct gcgcagatcg acgagctcga cgcgcagatt cttgcgacgt
    18841 gcgagccaca agagcccgtt gatcccgatc gcgccgcacg cctgctcgcc gacgagcttc
    18901 ggttcgcgcg cgacgatcgc cttgcacgtc atctgatcga tcgcgcggcc ctgcgcgtac
    18961 ggatgatagt gcgagagatc cgagctgatc acgatgagcg tctcgggtcc gccccacagc
    19021 gtctcgagga tgcggccgac agcttcgggc gccgcgcgcg atccacagag cggaacgatc
    19081 gtcgcgttcg gcaccacgcg ctgcagaaac ggcagctcga cctcgagcga gtgctcgcgc
    19141 gcgtgtgctg ctgcgttcgc cggaatgttc agcccaccga gcgcggtctc gtcgacggta
    19201 atctcgccga gcggcgtcgc gagcttcttc gcgccaggtg tcgctagccc atcgacaaac
    19261 acgcgatgcg cgggcccgac gagcacgacg cgcttgatgc gatcgccgta tggcgcgaca
    19321 cgcgcaaacg cggacgctgc gatcggacca gagtagacat aacccgcgtg cgggacgatc
    19381 aacgccttcg ggcagggccc cttcggcgcg tcgacgacgc cgagcaagcc gtcgatcgtc
    19441 gcctgcaatt cggcgcggtc gccgggatag aacgtgcccg ctaccgccgc tggtctaacg
    19501 ctatccgcca cgcccacgtg gctagcatag atctagcgag cagcgattcg cacgggaagc
    19561 gatgccggac catgcacgtg aaatggcgcg cgcggcgtcc actgcgtgct cgcgagctcg
    19621 atcgtgcgaa tccttggtag gagctcttcg agggcgaccc tcgcctcgag ccgcgagagc
    19681 ggcgccccta ggcagaagtg aatcccgtgc ccaaacgcga tgtgcggatt cggatcgcgc
    19741 ccgatatcga accgcgcagc ttcggggaag acggcgggat ctcgattcgc cgcgccgagc
    19801 atcggcagca ccatcttgcc agcaggaatc gtcacgccat gcagctcgac gctctgcttc
    19861 gtcgcgcgaa agatcgcctg cgcgggcgag cggtaccgca agacctcctc gatcgcgctc
    19921 ggcacgagcg tcacattgct ctgcagccgc gccatctggt ctggattctc gacgaggcag
    19981 atgatcgcgt tgtcgatcaa gttcgtcgtc gtctccgtcc cggcggcaag caacagctcg
    20041 aagaagcgaa cgagctcgag ctgactcaag tgctcgccgt cgatctctgc cgtcacgagt
    20101 cgggtcagca aatcgtccac gggctttgcg cgccgcgccg tgacgagctc accgagatac
    20161 gcctgcatct cgccatccgc gcgcagaaag tccgcgctcg ccgccgccga ctcgtgcgcg
    20221 ggccccatga tcgtcgcggc gagattcatg atcgcgcggc tccagccaat gagctttggc
    20281 caatcgccga gcggcaagcc aagcagctcc gcgatcacca tcatcggcag cggcgcggcg
    20341 agcttctcct cgaggtcaac ctcgccgcgt gcgacgactt catcgaccag cgccaccgcg
    20401 agctcccgaa tcctgggctc gagtgccgca atgctgcgcg acgtaaaggc ccgattgatg
    20461 atcgcgcgta gcttcgtatg ccgcggcgga tccatgaaca tcaaccactc gaagctgttg
    20521 ccgcgcgagg cacccaccgc cgagctgaag acggcggagt catgaagcgc gcgcttgacg
    20581 ctcgcgtagt cgaacaagaa gtagaggtcg gccggcgcga agtgaaaggc cggtgaggac
    20641 gcgcgtaact gctcgtagac gcgatacggg tcgcgccgca tggtgtcgtc gaagaagttc
    20701 atggcgggct cctagcgtta ctctagaacc ggcggccatg gcgcgcttga acgattcccg
    20761 gatgatcggc accgtgctgg ccccgcatct cgcggtgcgc gccacgacgc ggacattctc
    20821 gtcgccgcac gccgcgcacg gcatcgcgct gttcgtcggc ctcgagcacg acgtcgcgat
    20881 cacgacgccc ggcggccacg cccgcggccg cgctgtcatc gtggcgcccg acgtcgcgca
    20941 cagcgtctcg agccccggcc ccacgatcgg gatttgcttc gaccccgagg cgcttcctcg
    21001 tgtaacgggc ttcgcccgtg caatcggctc cgcccgcgct gccggctctg cccgctccga
    21061 tcggttcgct cgcgctgccg cctctgcgcg cgccgacggg ttcgctcgcg ccgacggctc
    21121 cgccagcgcg gaccttgtag tgggtccgta tggcggacaa gctaggtcac agcgcgagac
    21181 gaacgaacca cgccttgtcg cgtgtccggg tgacggacaa gctaggtctc ggcgcgagac
    21241 agacgacgcg agccttgtag cgtgtccggg tggcggacaa gccaacgtgt tggacggacg
    21301 gttggggcgt ttggtcttgg gcgcggcgtt agcacaccgg tcggagctcg gtagaggcga
    21361 tgtcctggcg ggcgtcggcg gcgagatcgc ggcggcgatc gggtcgaatg cgggtgcagc
    21421 ggaggcgcgg cttgatcgcc gggttgcgcg agtcgtcgaa gcgctgcgcg atccggcggc
    21481 ggagctgccg cggctgtcta tctcggacgc gcacctgcag gagctgttcg cgcgcgacgt
    21541 cggcgtgccg atccgcagct atcggctgtg gcaccggttg atgcgagcac tcgtgctgtt
    21601 cgcgcacggc gacgcgacga ccgccgcgca tgccgccggc ttcgcggacc tcgcgcactt
    21661 ctcgcgtacg tgccggcgat ttctcggcta ctcgccgacg acgttgcgca acggcgtttg
    21721 agtgatcggc gttgtctcga tcagccggcg cgacggcgtg aagcccatcg ggaattgcgg
    21781 acggcgcgag cgat
//
LOCUS       DATLBZ010000042         2777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_778253, whole genome shotgun sequence.
ACCESSION   DATLBZ010000042 DATLBZ010000000
VERSION     DATLBZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778253"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /gene="nuoD"
                     /locus_tag="VMZ53_02110"
     CDS             <1..1201
                     /gene="nuoD"
                     /locus_tag="VMZ53_02110"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HUS27264.1"
                     /translation="DLTDNETELPPDLMTVNMGPSHPAMHGTVRIVLTVDGERIVKGD
                     VQPGYLHRCFEKESEHATFTQVFPYTDRLNYVSPMINNCGWAMTIEKLMELTDRIPKR
                     VEYIRVIVSEMSRMTDHLTCVGASAMELGAFTPFLYFLKCREWLYELLEKVSGARLTH
                     TYVRIGGVAKDLPPDFHQDLTHTLGKIEKVMVEVEAMLNNNKIFRDRMAGIGAQTKEN
                     AIATGCVGPIARACGVDYDVRRDHPYSVYPELEFDVPLGTTGDCSDRYLVRVEELKQC
                     IRILRQCLAQIPEGPTMVDDHRVSLPPKRDTYNTIESMIRHFKHIMDGIRVPPGDTYS
                     FVEGGNGELGFFVVADGTGRPYKAYVRSPSFVHLSTASQLIHNHLIADVVPIFGMINM
                     IGGECDK"
     gene            1198..>2777
                     /locus_tag="VMZ53_02115"
     CDS             1198..>2777
                     /locus_tag="VMZ53_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HUS27265.1"
                     /translation="MTQSTSNAGPPAGTATPAPPTDPNNVTLTIDGTSVTVPKGTNVL
                     EAARKLGNDISAFCYHPGLKIVAQCRQCLVAVEKNPKLQPSCQQVVAEGMVVHTTDPQ
                     STLARKQQLEFTLLNHPIDCPICDKAGECTLQRLYFDHDNENSRVDVPKVHKAKVVDL
                     GPNIVLDQERCILCSRCIRTCDEVAGQHQLEFANRGDHEVLTTAPGEQLDNPYSLNTV
                     DVCPVGALTSKDFRFTMRAWELEATPSVCNGCATGCNVEIHHRNQRAWRLVPRHNPDV
                     NAYWMCDEGRFTYHDLRETRLAAPLVDGLPAGWDRSLQQAAKRLGVFLKDTSQIGVVL
                     SAVHTNEDNFALATLAKQWGLKTVYIAGKPPVPAREDGKLRVADINPNMNGVKTIAEA
                     LGLTTTWALELATNIPANLRALVILGDVLPGVDAGKLKELEVISIATHERGPVPHAKV
                     ALPAATWAETGATTTNVKGIVQRMHAAFGPPGQAIAGWEAVVRLAQATGVKLAWTHAR
                     EVFKDMTASVPAWKELTWA"
BASE COUNT          526 a          947 c          868 g          436 t
ORIGIN      
        1 ggacctcacc gacaacgaga ccgagctgcc gcccgatctc atgaccgtga acatgggccc
       61 ttcgcatcct gcgatgcacg gcaccgttcg catcgtgctg accgtcgacg gcgagcgcat
      121 cgtcaagggc gacgtccaac ccggctatct gcatcgctgc ttcgagaagg agagcgagca
      181 cgcgacgttt acgcaggtgt tcccgtacac ggaccgcctc aactacgtct cgccgatgat
      241 caacaactgc ggctgggcga tgacgatcga gaagctcatg gagctgaccg atcgcatccc
      301 gaagcgcgtc gagtacatcc gcgtgatcgt cagcgagatg agccgcatga cggatcacct
      361 gacgtgcgtc ggcgcgtcgg ccatggagct cggcgcgttc acgccgttcc tctacttcct
      421 caagtgccgc gagtggctgt acgagctgct cgagaaagtc tccggcgcgc gcctcacgca
      481 cacgtacgtg cgcatcggcg gtgtcgcgaa ggatctcccg cccgacttcc accaggacct
      541 cacgcacacg ctcggcaaga tcgagaaggt gatggtcgag gtcgaggcga tgctgaacaa
      601 caacaagatc tttcgcgatc gcatggccgg catcggcgcg cagacgaaag agaacgcgat
      661 cgcgacgggc tgcgtcggtc cgatcgctcg cgcatgtggc gtcgactacg acgtccgccg
      721 cgatcatccg tactccgtct atcccgagct cgagttcgac gtgccgctcg gcaccacggg
      781 cgactgctcc gaccggtacc tcgtccgcgt cgaggagctg aagcagtgca tccgcatctt
      841 gcggcagtgc ctcgcgcaga tccccgaagg cccgacgatg gtcgacgatc accgcgtgtc
      901 actgccgccg aagcgcgaca cgtacaacac gatcgagtcg atgatccgtc acttcaagca
      961 catcatggac ggcatccgcg ttccgcccgg cgatacatac tcgttcgtcg agggtggcaa
     1021 cggtgagctc ggcttcttcg tcgtcgccga cggcaccggc cgcccgtaca aggcgtacgt
     1081 ccgttcgccg tcgttcgtgc atctctcgac cgcgagccag ctgatccaca accacctcat
     1141 cgcggacgtc gtcccgatct tcggcatgat caacatgatc ggcggggagt gcgacaagtg
     1201 acgcaatcga cttcgaacgc gggcccgccc gcaggcaccg cgacgccggc accgccgacc
     1261 gatccgaaca acgtgacgct cacgatcgac ggcacgtcgg tcaccgttcc gaagggcacg
     1321 aacgtgctcg aggctgcgcg caagctcggc aacgacatca gcgcgttctg ctaccacccc
     1381 ggtctcaaga tcgtcgcgca gtgccggcag tgcctcgtcg cggtcgagaa gaacccgaag
     1441 ctgcagccga gctgccagca agtcgtcgcc gagggcatgg tcgttcacac gacggatccg
     1501 cagtcgacgc tcgcgcgcaa gcagcagctc gagttcacgc tgctgaacca tccgatcgat
     1561 tgtccgatct gcgacaaggc cggcgagtgc accctgcagc gcctgtactt cgatcacgat
     1621 aacgagaatt cgcgcgtcga cgttccgaag gtccacaagg cgaaggtcgt cgacctcggt
     1681 ccgaacatcg tgctcgatca agagcgctgc atcctctgct cgcgctgcat tcgcacgtgc
     1741 gacgaggtcg cgggccagca tcagctcgag ttcgccaatc gcggcgatca tgaagtactc
     1801 acgacggcac ccggcgagca gctcgacaac ccgtactcgc tcaacaccgt cgatgtctgc
     1861 ccggtcggcg cgctgacgtc gaaggacttc cgcttcacga tgcgcgcgtg ggagctcgag
     1921 gcaacgccga gcgtgtgcaa tggctgcgcg acgggctgca acgtcgagat ccatcaccgc
     1981 aaccaacggg cgtggcgcct cgttccgcgt cacaaccccg acgtcaacgc gtactggatg
     2041 tgcgacgagg gccgcttcac gtatcacgac cttcgcgaaa cgcgcctcgc tgcgccgctc
     2101 gtcgacggtc tgcccgccgg ttgggatcgg tcgctgcagc aggcagccaa gcgcctcggc
     2161 gtgttcctca aggacacgtc gcagatcggc gtcgtgctgt cggccgtcca cacgaacgag
     2221 gacaacttcg cgctcgcgac gctcgccaag cagtggggcc tcaagacggt gtacatcgcc
     2281 ggcaagccgc cggtgcctgc acgcgaggac ggcaagctgc gcgtcgccga catcaacccg
     2341 aacatgaacg gcgtgaagac gatcgcagaa gcgctcggtc tcaccacgac gtgggcactc
     2401 gagctcgcga cgaacattcc cgcgaacctc cgcgcgctcg tcattctcgg cgatgtgctg
     2461 ccaggcgtgg atgcaggaaa gctcaaggag ctcgaggtca tctcgatcgc gacgcacgag
     2521 cgcggtccgg tgccgcacgc gaaggtcgcc ctgcccgccg cgacgtgggc agagaccggc
     2581 gcgacgacga cgaacgtcaa gggtatcgtc cagcgcatgc acgcagcgtt cggcccgccc
     2641 ggccaggcga tcgccggctg ggaagcggtc gtcaggctcg cgcaggcgac cggcgtgaag
     2701 ctcgcctgga cgcacgcgcg cgaggtcttc aaggacatga cggccagtgt gccggcgtgg
     2761 aaggagctca cgtgggc
//
LOCUS       DATLBZ010000043         7374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3759992, whole genome shotgun sequence.
ACCESSION   DATLBZ010000043 DATLBZ010000000
VERSION     DATLBZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3759992"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VMZ53_02120"
     CDS             <1..909
                     /locus_tag="VMZ53_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27266.1"
                     /translation="RAASSGNPERLAVDEQVTIGISCESCHLGGRAHADGAKISSLPA
                     GAGVHVRADAPAMPATFELQRKDAHVVNTICAQCHSGPSPRLADGTALRNSSEALDLA
                     AGPCTTLKCTDCHDPHVADSRADEPRSIAACVRCHSQLAAPESATAHAGAGHSTSTCL
                     DCHMPKIVMGIDRFVRTHRISSPTNPQLLAEGSPNACNVCHLDRSIDWTLDELDRGWD
                     TKLAKRVSTKVYRDNAVGEVWLASKSPALRLVATQAYARSPKLAAYVMPQLRKGLADP
                     LAYVRAWTQLALDALRAAQPKLPTAN"
     gene            complement(916..1500)
                     /locus_tag="VMZ53_02125"
     CDS             complement(916..1500)
                     /locus_tag="VMZ53_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27267.1"
                     /translation="MDAPRDAAVDAPIDAPIDAPPAPLKCKWRNYYTAEDFGASVGYG
                     AEVPFGDLESPISASCCDVPNSQVLCTFVDKVWEEVPGPLGTSNIPVFLEHAELFERH
                     GKKPLRTAILSAFHPGPMNDPFGKSASPPLFRARVELDSAALHVVTKDDRLCHGRPLP
                     CSKAMIEERECGPYRRRFASLVQKFCDGLGASAW"
     gene            complement(1643..2593)
                     /locus_tag="VMZ53_02130"
     CDS             complement(1643..2593)
                     /locus_tag="VMZ53_02130"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUS27268.1"
                     /translation="MKRAFVTGGSGFVGGRLITALRARGVSVSALARSDKSAATVEAL
                     GASAARGDLDDVAALQAGMQGCDVVFHAAAHTDQFDPLAVHMRITTRGTENAINAAHA
                     AGVKRFIHVSTEAVLADGNPIIRADETRPLPAKPMGAYPLTKGLAEAIVLQANRAGFE
                     TVIVRPRFVWGKGDTNLLPEFVEAVRRGKFAWISGGHYLTSTTHVDNTVEGLLLAADK
                     GKPGEIYFVTDGTPVEFREFITRLLATQGIVAPVRSIPHWVGRMVVAMSFWMKRPPLT
                     ATAFSLMAHEVTLDDTKARRDLGYTSAKSLDAGFAEMKPL"
     gene            2622..4118
                     /locus_tag="VMZ53_02135"
                     /pseudo
     CDS             2622..4118
                     /locus_tag="VMZ53_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
     gene            4343..4600
                     /locus_tag="VMZ53_02140"
     CDS             4343..4600
                     /locus_tag="VMZ53_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27269.1"
                     /translation="MDRLAEVSCKPDQTLDAFKAAHGQDEAQPELGRAAFKHGMTVDF
                     YATNVDCAVSVRSETDAAAALALAKAVDPALTATTAPGAAK"
     gene            4722..5675
                     /locus_tag="VMZ53_02145"
     CDS             4722..5675
                     /locus_tag="VMZ53_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27270.1"
                     /translation="MGNTDRFSPGSRIGDYVVERESGDGYEAVHVLLPRRVRLEVMHP
                     TFVGLKPVAVRMMREACILEALHHPGVPRVFEVGMMNDQRTNRPWVASELVDGQLLVA
                     SAPLSPIETLELIRDLADIIAHAHDRGVVHRALRVDTVARCENRGFPLCVVDWSDARV
                     DASDRARADDVFALGVIAYQALTGSLPNMPTARRAPGAPARLCLMIDDLLVANPMSRP
                     SAAEVRARAKLILETLDDGEPVVEEQISEADLVELTGFESTAQQMPALRTRWTPPHGV
                     TTAPPPLPPTSGIAIARSTVLDRAASPEGMRSTIGVLKPRS"
     gene            complement(5672..6442)
                     /locus_tag="VMZ53_02150"
     CDS             complement(5672..6442)
                     /locus_tag="VMZ53_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27271.1"
                     /translation="MRLSSLLLLLLPSVALAQAPGETPPSPPPDPAQEPDVHPPAPAP
                     APEPAPTPEATAAPAPAPTPAPTPAPVASPMTQLTPDAVPEQCKALAKEATGPSINRA
                     LSARISLASCLADHKLKPLVLCDCEASVIDINAATQLSIALLDEVFTKGDAAMQILAR
                     QAKGDMLSALAQRMQATVPPPVTATPEAIALRDTRLDLLQPMIEPWQTQARAAYSEVD
                     KIAKANPQLSKNAAVVAAVRASRTKLGAQPPATQTATR"
     gene            complement(6541..6915)
                     /locus_tag="VMZ53_02155"
     CDS             complement(6541..6915)
                     /locus_tag="VMZ53_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27272.1"
                     /translation="MTKLEDLDDETLRGVSGGINMKLTQYGYPNDPYSDSNTRHGRGA
                     YHSLRNESMAITDSGLRALGLSRRDAMGGNHWVELRLRGGGVLQRRIDDRAPQGEMRA
                     DLYQPHGFDRRLPDRADVRLLR"
     gene            complement(7051..>7374)
                     /locus_tag="VMZ53_02160"
     CDS             complement(7051..>7374)
                     /locus_tag="VMZ53_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUS27273.1"
                     /translation="TRVATYADAIKMVNSNPYANGVAIFTNDGGAARKFQHEVEVGMV
                     GINVPIPVPMAYYSFGGWKASLFGDLHIYGPDGVHFYTRMKAVTSRWPDPRHRGVDLG
                     FPQNK"
BASE COUNT         1211 a         2429 c         2482 g         1252 t
ORIGIN      
        1 cgagcggcga gcagcggcaa tcccgagcga cttgcggtcg acgagcaagt gacgatcggc
       61 atcagctgcg agagctgtca cctcggcggg cgcgcgcatg ccgacggcgc gaagatctcg
      121 tcgctgcccg cgggcgctgg agtgcacgtt cgcgcggacg cacccgcgat gccggcgacg
      181 ttcgagctgc agcgcaagga tgcgcacgtc gtcaacacga tctgcgcgca gtgtcactcg
      241 ggtccatcgc cgcgactcgc ggatggcacc gcgcttcgca actcgagcga ggcgctcgat
      301 ctcgcagcgg gaccgtgcac gacgctgaag tgcactgact gccacgatcc gcatgtcgcc
      361 gactcgcgcg ccgacgagcc gcgatcgatc gccgcgtgcg ttcgctgtca ttcgcagctc
      421 gctgcacccg aatccgccac cgcccacgcg ggcgcaggtc actcgacgtc aacgtgcctc
      481 gattgccaca tgcccaagat cgtcatgggc atcgatcgct tcgtgcgcac gcatcgcatc
      541 agctcgccga cgaatccgca gctgctcgcc gagggctcgc cgaacgcgtg caacgtctgt
      601 cacctcgatc gctcgatcga ctggacgctc gacgagctcg atcgcggctg ggacacgaag
      661 ctcgcgaagc gcgtttcgac gaaggtctat cgcgacaatg cggtcggcga agtctggctc
      721 gcgtcgaaga gcccggcgct gcgcctcgtc gcgacgcagg cgtacgcgcg atcgccgaag
      781 ctcgcagcgt acgtcatgcc gcagctgcgc aaggggctgg ccgatccgct cgcctacgtc
      841 cgcgcgtgga cgcagctcgc cctcgacgcg ctacgcgctg cgcagccgaa gctgccgacc
      901 gcaaactgag acgcgctacc acgcgctcgc gcccaagccg tcgcagaact tctgcacgag
      961 gctcgcgaag cggcggcgat agggaccgca ctcgcgctct tcgatcatcg ctttgctgca
     1021 aggcagtgga cggccatggc acagccgatc gtccttcgtg acgacgtgca gcgcagcgct
     1081 atcgagctcc acacgcgcgc ggaacagcgg cggcgatgcg gatttgccga acggatcatt
     1141 catcggccca ggatggaagg cggacaggat cgcggtccgc agtggcttct tgccgtgacg
     1201 ctcgaacagc tccgcgtgct cgaggaagac cgggatgttg gaagtcccga gcggtcccgg
     1261 cacttcctcc cacactttgt cgacgaaggt gcagagcacc tgcgaattcg gcacgtcgca
     1321 gcacgacgcg ctgatcggtg actcgagatc gccgaacggc acctcggcgc cgtaaccgac
     1381 cgacgccccg aagtcttccg ccgtgtagta gttccgccat ttgcatttga gcggggctgg
     1441 gggcgcatcg atcggcgcgt cgatcggcgc atcgactgcc gcgtcgcggg gcgcgtccac
     1501 ccgtgcgtcg tcggcgataa gcagagcagg gggcgcgtgg tccgcgcgtt ggcacgcggt
     1561 cacgacgatc aggaaggcac gaagtgcgcg tgtcacgcga acgcgacacc gcggcgatat
     1621 cgaggttgcg agaacgcgcc gtttatagcg gcttcatttc agcgaacccg gcatcgagcg
     1681 attttgcgct cgtgtagcca aggtcgcgcc gcgccttggt gtcgtcgagc gtcacttcgt
     1741 gcgccatcag ggagaatgcg gtcgcggtga gcggcggtct cttcatccag aacgacatcg
     1801 cgacgaccat ccgcccgacc cagtgcggga tgctgcgcac gggcgcgacg attccctgtg
     1861 tcgcaagcag cctcgtgatg aactcgcgga actcgacggg cgtgccgtcc gtgacgaagt
     1921 agatctcgcc tggcttgccc ttgtcggcgg cgagtagcag gccttcgacg gtgttatcga
     1981 cgtgcgtcgt cgacgtcagg taatggccgc cggaaatcca cgcgaacttg ccacggcgca
     2041 cggcctcgac gaactccggc agcaagttcg tatcgccttt gccccagaca aagcgcgggc
     2101 gaacgatcac ggtctcgaac cctgcgcgat tcgcctgcag cacgatcgcc tcggcaagcc
     2161 ccttcgtcag cgggtacgca cccatcggct ttgcggggag agggcgtgtc tcgtcggcgc
     2221 gaatgatcgg attgccatcg gcaagcaccg cttcggtgct gacatggatg aagcgcttca
     2281 cgccggccgc atgcgccgcg ttgatcgcgt tctccgtccc cctcgtcgtg attcgcatgt
     2341 gcaccgcgag tggatcgaat tggtcggtgt gcgcggccgc atgaaacacg acgtcgcagc
     2401 cctgcatgcc ggcttgcagc gcggccacgt catcgagatc gccgcgcgcg gcgctcgcac
     2461 cgagagcctc gacggtcgcc gccgacttgt cggagcgcgc gagggcgctg acgctcacgc
     2521 cacgggcgcg aagcgcagtg atgagacgac cgccgacgaa acccgagcca ccggtgacga
     2581 atgctcgctt cacgcggcgg aatctactag gttcgcgaat catgagcgcg gttggcgcgg
     2641 agattcatca cgacgacggg cgagtcgagc tgcgtgaccc cgagcagctg aaggcatcgc
     2701 cgctctataa cgaggacctc gcgccggtcg cggtcgcgaa gcgcacctgg aacacgtggg
     2761 actacgccgc gctgtggatc tcgatggccc actgcattcc gacgtacacg ctcgcgtcgt
     2821 tcatgatcat gcaggggctt gcgtggtggc aggcgctcac cgcgatcttg atcggcaatg
     2881 tgatcgtgct cgcgccgatc ctgctcaact cgcaccccgg tacgaagtac ggcattccgt
     2941 ttccggtgtt cgcgcgtgcg gcgtacggca cgagcggcgc gaacttgccg gcgctcatgc
     3001 gcgcgctgat cgcgtgcggc tggttcggca tcaacgcgtg gatcggcggg caggcgctgc
     3061 aagtgttctt cgtgtcggtg tggcgcgact gggcgacgct gatgggcacc tccagctatc
     3121 cgttcgagca ttttcagaac acgcagtggg tctcgttcgc gctgttctgg ggcctcaaca
     3181 tcctgatcat cttccgcggc atggacgtgc ttcgccgcgt cgagcggttc gcggcgccgt
     3241 acgtgctcgt gatgaccgcg gcgctgctcg cgtgggcgat ctacaaagcg ggcggcctcg
     3301 gcccgatcct cgagaaccac tcgttccaca gcaagaacgg caagaccgtc tcgcaggtga
     3361 tgattcccgc gctgaccggg acgatcgcgt tctggtcgac gctttcgctc aacatgcccg
     3421 acttcacgcg gttcgggcgc agccagcgcg agcaagcgat cggtcaaacc gtcgcgctgc
     3481 cgtcgacgat gacggtgttt gccgcgatga gcatcatcat cacgagcgcg accgtgatca
     3541 tctatggcga cgcgatttca gatcccatca cgctcggcag caagttcggc agccgcatca
     3601 tcgtcgctgt cgccatgttc acggcggtga tcgcgacgct cgcagtgaac atcgcggcga
     3661 acgtcgtgtc gccggcgaac gacttcgcga acgcgttccc gaagaagatc gacttcaagc
     3721 tcggtggtct catcaccggc gtcatcggcg tctgcatgat gccgtgggag ctgctgagag
     3781 acggcgagcg ctacatcaac ggctgactcg gcggctacgg cgcagcgctc ggctcggtcg
     3841 gcggcgtgct catcgtcgac tactggatct tgcgcaagaa gaacctcgac ctgaagtcgc
     3901 tgtacgtcac cgacggtgcg tatcgctatc agggcggctg gaacatgaac gcggtgatcg
     3961 cgacgctcct cggtgcgggc ctcgcgctgg cgggcgcgtt ctggaaaccg ctgcacgtca
     4021 tctatgactg gtcgtggttt gtcggctttg gcgtcgcggg cgggctctac tggctactga
     4081 tgggatcgtc cgtgccccag cgaaaggcag aagtatgaag aagtcgatcg cgattgctct
     4141 cgttgtgctc gctggctgcg gttcgaagaa agagtcgaat caagacaaac ccgctacggg
     4201 tggacctgca gcgcccgctg cgcccacggc cggctcgcgg tgcgacgcgc tattgcccga
     4261 gaatgtgcga acgggacaca tggtccgcgg cgacagcgct tcggatcgcg agttgcggtg
     4321 ccggatctat gacggtaacc gaatggatcg cctcgccgaa gtgagctgca agcccgacca
     4381 aacgctcgac gcgttcaaag cggcccacgg gcaggacgag gcgcagcccg agcttggccg
     4441 cgcggcgttc aagcacggca tgaccgttga cttctacgcg acgaacgtgg actgcgcggt
     4501 gagcgtcagg agcgagacgg atgccgcggc agcgctggct ctcgcgaagg cggtcgatcc
     4561 ggctctcacg gcgacgacgg cgcccggcgc cgcgaagtaa cggcgatgag gtcagccgtg
     4621 ccctgacaaa acgcggctga ggtcagctgt cccggggggt ggctcctgca gcttcgcggc
     4681 gttaggcggg gcacagcggg tgcactgatg cccgagcgat gatggggaac accgaccgct
     4741 tctcgcccgg cagccgcatc ggagactacg tcgtcgaacg ggagtccggc gacggctacg
     4801 aggccgtcca cgtgttgctt ccgcggcgtg ttcgtctcga ggtcatgcac ccgacgttcg
     4861 ttggcctgaa gccggtcgcg gttcgcatga tgcgcgaggc gtgcatcctc gaagcgcttc
     4921 atcatcccgg cgtgccgcgc gtgttcgaag tcggcatgat gaacgaccag cgcacgaatc
     4981 gtccgtgggt cgctagcgag ctcgtcgatg gtcagctgct ggtcgcgagc gcgccgctct
     5041 ctccgatcga aacgctcgag ctcatccgcg atctcgcaga catcatcgcg cacgcgcacg
     5101 accgcggagt cgtccatcgc gcgcttcgcg tcgacacggt cgcgcggtgc gagaatcgcg
     5161 gcttcccgct gtgtgtcgtc gactggagtg acgcacgcgt cgatgccagt gaccgcgccc
     5221 gcgccgatga cgtgttcgcg ctcggcgtga tcgcgtatca agcactgacc ggcagcctgc
     5281 cgaacatgcc gacggctcgt cgcgctccgg gggcgccggc gcgcctctgt ctgatgatcg
     5341 acgatctgct cgtcgcgaat ccgatgagcc gtccgtcggc cgccgaggtt cgcgctcgcg
     5401 ccaagctcat cctagagacg ctcgacgacg gcgagcccgt tgtcgaagaa cagattagcg
     5461 aagccgacct cgtcgagctc accggcttcg agtccaccgc gcagcagatg ccagccctgc
     5521 gtacgcggtg gactcctccg catggcgtca cgaccgcacc acctccgctc ccgcccacct
     5581 ctggcattgc gatcgcaagg tccaccgtgt tggaccgcgc agcatccccg gaggggatgc
     5641 ggagtacgat cggcgtcctg aagccgcgct cctagcgagt cgcggtctgc gtcgcgggcg
     5701 gctgcgcgcc gagcttcgta cggctcgccc gcaccgcggc gaccacagcc gcgttcttcg
     5761 agagctgcgg gttggccttc gcgatcttat cgacctcgct gtacgcggcg cgagcctgcg
     5821 tctgccacgg ctcgatcatc ggctgcagca agtcgaggcg cgtgtcgcga agtgcgatgg
     5881 cttcgggcgt cgcggtaacc ggcggcggca ccgtcgcctg catgcgctgc gcgagcgccg
     5941 agagcatgtc gcccttggct tgccgcgcga ggatctgcat cgcggcatcg cccttcgtga
     6001 agacctcgtc gagcagcgcg atcgacagct gcgtcgccgc gttgatgtcg atgacagacg
     6061 cttcgcagtc gcacaggacg agcggcttga gcttgtgatc ggcgaggcag ctcgcgagcg
     6121 agatgcgagc agacagcgcg cgattgatgc tcggcccggt cgcctctttt gcgagcgcct
     6181 tgcactgctc cggcactgcg tcgggtgtca gctgcgtcat cggcgacgca accggagcag
     6241 gcgtcggagc aggcgtcgga gcaggcgccg gtgccgccgt cgcctcgggc gtcggtgcgg
     6301 gctcgggcgc gggcgcgggt gccggcggat ggacatcggg ctcctgcgcc ggatcgggcg
     6361 gcggagaagg tggcgtctca cccggagctt gcgcaagagc aacgctcgga agcagaagga
     6421 gcaacaacga cgacagacgc atgcctcgct tgaagtgcag cggtcgtgcc gcgcggatcg
     6481 tgccgccagg aaatcgtcgg cgtgcggacc tgctacaccg aggttctcgc agggactgcg
     6541 ctaacgcagc agtcgcacgt cggcgcggtc gggcaacctg cggtcgaagc cgtgcggctg
     6601 gtagaggtca gcacgcatct cgccttgtgg tgcgcgatcg tcaatgcgcc gctggagcac
     6661 gccgccgccg cgcagtcgca gctcgaccca gtggttgccg cccatcgcat cgcgtcgcga
     6721 cagaccaagc gcgcggagac ccgaatcggt gatcgccatc gactcgttgc ggagcgagtg
     6781 gtacgcgccg cgaccgtgcc gcgtgttcga gtccgagtac ggatcgttcg ggtacccgta
     6841 ctgcgtgagc ttcatgttga tgccgcctga gacaccgcga agcgtttcgt catcgaggtc
     6901 ttcgagcttc gtcatgctga cggctatcgc aatggcgatg ccacggctgc cgcgcgttcg
     6961 gcagcagccc gaccccgtca agtcgcgacg ttgtcacgcc gtcaaccgcg ggccgcaatg
     7021 tgcccagtca ccggctagtg acccagccag ctacttgttc tgcgggaagc cgagatcgac
     7081 gccgcgatga cgcggatcgg gccagcgcga cgtgacggcc ttcatgcgcg tgtagaagtg
     7141 cacgccgtcg gggccgtaga tgtggaggtc gccgaacagc gacgccttcc agccgccgaa
     7201 cgagtagtac gccatcggca ccggaatcgg cacgttgatg ccgaccatgc cgacctcgac
     7261 ctcgtgttgg aacttgcgcg ccgcgccgcc gtcgttcgtg aagatcgcga cgccgttcgc
     7321 gtacgggttc gagttgacca tcttgatcgc gtccgcgtac gtcgccacgc gcgt
//
LOCUS       DATLBZ010000044         2909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_779963, whole genome shotgun sequence.
ACCESSION   DATLBZ010000044 DATLBZ010000000
VERSION     DATLBZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779963"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VMZ53_02165"
     CDS             complement(<1..1090)
                     /locus_tag="VMZ53_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HUS27274.1"
                     /translation="MRSVVLLAGLAACDFVHGALNSSVDAGGDSDSEIDASIDAPPMT
                     TAVKFTTVTSTLGSVRPGAYGWQVTAVLRNELPDDITNIRPTLTFTIGTEDRASSFRW
                     RDIDMREAVATLPATTLASNSEVTYKFVVDALPWLMGPGTLQINGAATFDSTGASLSA
                     TPAMTPVSLPFVAMNAPIVVTTLTDEMTNNTTTSLREAITQARAVPGLDHITFDPAVF
                     TAGSTSTLSSTLGELPTLDGANDQIVIDGHGANVTLVVGTAWQSSSRWPFRVTGGQAV
                     IAGIRFKDFGYNYPAEDVSSDNCGSGNHNEGGAIRVDGGTLTLEGNTFDDGNVAERNC
                     YASLLRYEGGTNHRVINNRWTNQSQDAMY"
     gene            complement(1136..1711)
                     /locus_tag="VMZ53_02170"
     CDS             complement(1136..1711)
                     /locus_tag="VMZ53_02170"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HUS27275.1"
                     /translation="MKLAALALLALATSTASAEAYLPKKDRERALKSTKHGAVLAPGT
                     KPKKLVNLYNEHTKDWLAVEAESADPPSAATVSIFLRDHFTNMATKMEPKLISVAVSA
                     AKQFKSDLVNVVSGFRHPKYNLMLRKKGHEVARDSQHTHGNAIDFALPTCSTKQLEKW
                     AKDQKIGGVGVYMQSGFIHMDTGPIREWAGE"
     gene            complement(1708..2550)
                     /gene="larE"
                     /locus_tag="VMZ53_02175"
     CDS             complement(1708..2550)
                     /gene="larE"
                     /locus_tag="VMZ53_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HUS27276.1"
                     /translation="MSSVKLAALRDTLAGYGQVVVAFSGGVDSAFLLKVAIDALGEKC
                     HAVTAVSPTMARSEIRAASELAAEMGLGARHHVVDSHELEVPGFADNPTHRCALCKTE
                     LMGVATPIAEKLGGAPIVLGTNMDDLGDVRPGIAAAREKGARMPMVDVGLTKQEIREL
                     SRELALPTWDKPQLACLSSRFPYGTKITPDRLRRVDDFEDGLRELGFKQLRVRFHDSI
                     ARLEIDGDDLVRIVAPGVRESVVALGKRLGFAYVALDLAGFRSGSLNEVLPSDTLVKL
                     KRPS"
     gene            complement(2679..>2909)
                     /locus_tag="VMZ53_02180"
     CDS             complement(2679..>2909)
                     /locus_tag="VMZ53_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HUS27277.1"
                     /translation="TTARPALVIYHSHTNGRAYFSDTDRNQSATTDAPHYDCQHLVVG
                     VNAERVIEAAQFAWSWDDKGFVEIARWQIGDA"
BASE COUNT          474 a          957 c          941 g          537 t
ORIGIN      
        1 tgtacatcgc gtcctgcgac tgattcgtcc accggttgtt gatgacgcga tgattcgtgc
       61 cgccctcgta acgcagcagc gacgcgtagc agttgcgctc ggcaacgttg ccgtcgtcga
      121 acgtgttgcc ttcgagggtc agcgtgccgc cgtcgacgcg aatggcgccg ccttcgttgt
      181 ggttgccgct gccgcagttg tccgacgaga cgtcctcggc cggatagttg tagccaaagt
      241 ccttgaagcg gatgccggcg atcacggctt gcccgccggt cacgcgaaac ggccagcgag
      301 agctcgactg ccacgccgta ccgacgacca gcgtcacgtt cgcgccatgg ccgtcgatca
      361 cgatctggtc attggctccg tcgagtgtcg gcagctcgcc caatgtggag ctcagcgttg
      421 aggtgctgcc cgctgtgaag accgccgggt cgaacgtgat gtgatcgaga ccgggcacgg
      481 cgcgcgcctg cgtgatcgct tcgcgcaagc tggtcgtcgt gttgtttgtc atctcgtcgg
      541 taagtgtcgt cacgacgatc ggtgcgttca tcgcaacgaa cggaagcgac accggtgtca
      601 tcgctggagt cgcgctgagc gatgcgcccg tcgagtcgaa cgtcgcggca ccgttgatct
      661 gcagtgtgcc cggacccatg agccatggca gcgcgtcgac gacgaacttg taggtgacct
      721 cgctgttgga tgcgagcgtc gtcgcgggaa gtgtcgcgac cgcttcgcgc atatcgatgt
      781 cccgccaccg aaagctcgac gcgcgatcct cggtgccaat cgtgaaggtc agcgtcggac
      841 ggatgttcgt gatgtcgtcc ggaagctcgt tgcgcaggac cgcggtcacc tgccagccgt
      901 acgcgccagg tcgcacggag ccgagcgtcg acgtcacggt cgtgaacttc acggccgtgg
      961 tcatcggcgg cgcgtcgatg gatgcgtcga tctcgctgtc gctatcgccg cccgcgtcga
     1021 cggacgagtt cagcgcgccg tgaacgaagt cgcacgcagc caatcccgcg agcaaaacaa
     1081 cgctgcgcac gcttcgatgc tatcagtcgc ctggccgcgg acgtggcttt tgtagctact
     1141 cgccggccca ctcgcggatc gggcccgtat ccatgtggat gaagccgctc tgcatataca
     1201 cgcccacacc gccgatcttc tggtccttcg cccacttctc gagttgcttc gtcgagcagg
     1261 tggggagggc gaaatcgatg gcgttgccgt gtgtgtgctg gctgtcgcgc gcgacctcgt
     1321 ggcccttctt gcggagcatc aagttgtatt tcgggtgccg gaagcccgag acgacgttga
     1381 cgaggtcgct cttgaactgc ttcgccgccg agaccgcgac gctgatgagc ttcggctcca
     1441 tcttcgtcgc catattcgtg aagtgatcgc gcagaaagat gctcacggtc gctgcgctcg
     1501 gcggatccgc gctctcggcc tcgacggcga gccagtcctt cgtgtgctcg ttgtagaggt
     1561 tgacgagctt cttcggcttc gtgccaggcg cgaggacggc gccgtgcttc gtcgacttca
     1621 gcgcgcgctc gcgatccttc ttcggcaagt acgcttcggc tgacgcggtc gaggtcgcca
     1681 gcgcgaggag cgcgagcgcc gcgagcttca cgacggcctc ttgagcttca cgagtgtgtc
     1741 actcggcagg acttcattga gcgagcccga ccgaaagccg gcgagatcga gcgcgacgta
     1801 cgcaaagccc aggcgcttgc cgagcgcgac gacgctctcg cgaacccctg gcgcaacgat
     1861 tcgcacgaga tcatcgccgt cgatctcgag tcgcgcgatc gagtcgtgaa agcgcacgcg
     1921 cagctgcttg aagccgagct cgcgcaggcc atcctcgaag tcgtcgacgc ggcgcagccg
     1981 atccggcgtg atcttcgtgc cgtacggaaa ccgcgacgac agacacgcga gctgcggttt
     2041 gtcccaggtt gggagcgcga gctcgcgcga cagctcgcga atctcttgct tcgtcagccc
     2101 gacgtcgacc atcggcatgc gcgcgccctt ctcgcgagcc gccgcaatgc ctggccgcac
     2161 gtcgccgagg tcgtccatgt tcgtgccgag cacgatcggt gcgccgccga gcttctcggc
     2221 aatcggcgtc gcgacaccca tcagctccgt cttgcacagc gcgcagcgat gcgtcgggtt
     2281 gtccgcaaag cccggtacct cgagctcgtg cgagtcgacg acgtgatgcc gcgcgccgag
     2341 ccccatctcc gccgcgagct cactcgcagc gcgaatctcc gaccgcgcca tcgtgggcga
     2401 cacggcagtc accgcgtggc acttctcgcc cagcgcatcg atggcgacct tgagcagaaa
     2461 tgccgaatcg acgccgcccg agaacgcgac gacgacctgg ccgtagccag ccagcgtgtc
     2521 acgcaatgct gcgagcttca ccgaggacat cgaaatcagc ctagcacggg cgatgcggct
     2581 cgctcgacga caacctcgtc agcctcggtg acccgtgagc cctcggtgac ccgtgagccc
     2641 tcggagatcc gccaccgcgc gatttcgacg agacaccgtc aggcatcgcc gatctgccac
     2701 cgcgcgattt cgacgaagcc cttgtcgtcc cacgaccatg cgaactgcgc ggcctcgatc
     2761 acgcgttcgg cgttcactcc gacgacgagg tgctggcagt cgtagtgcgg ggcgtcggtc
     2821 gtcgccgact gattgcggtc cgtatcggag aagtacgcac ggccattggt gtgcgagtga
     2881 tagatgacga gggcagggcg cgccgtcgt
//
LOCUS       DATLBZ010000045        15221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5980734, whole genome shotgun sequence.
ACCESSION   DATLBZ010000045 DATLBZ010000000
VERSION     DATLBZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15221
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5980734"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VMZ53_02185"
     CDS             complement(<1..377)
                     /locus_tag="VMZ53_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27278.1"
                     /translation="MSDAFDDPDQVPAASDLAEVPQAPGAALASAATAATEEAEGRAP
                     DTVEGRRSEGLRRERVVASKQYRDGTFHNTSGVSESLRPEAADGQRRGTGWMLREWMF
                     GGQERRPKVVLPVERPHEAWTRP"
     gene            complement(473..2077)
                     /locus_tag="VMZ53_02190"
     CDS             complement(473..2077)
                     /locus_tag="VMZ53_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS27279.1"
                     /translation="MPSTTPSAMSSQTSSTTSSQMSATTPASHYDYVIVGAGMGGLTV
                     GALLANAGARVAIVEAHEYPGGCCHTFPMGDYRFCAAVHYIFSCGEGEPVHNLLRALG
                     LHETITFERLNPDGYDHFRCPSAGLTFRIPSGLDAWCTRLSEQFPAETKAITAFFSTI
                     KTLATELGRLPENLTGLRILKGLIAAPHVALHRASTLQNLFDKLRLSAPVQAILSTQL
                     GDAGLPPRDISLLLYTALISKYCAGAYYPTEHFASLIEGVANAVTSKKGCDIFYNTEV
                     ASVRMEGGRVAAVVATDGREFRGAKIICNADPKWFVRLLGEQHFPRDYMKRVNYDYSA
                     SSFSLYLGVRGIDLREHGFGNWNVWHYPSLDINAVYDAQHERDDLDKPWLFMSTPTLC
                     SPNAKKRHAPEGEQILELITTCAYKPFDERSARDRKEYTRRKVAIRDRMIATVQEHYV
                     PNLRKHLAMRVAGTPTTNRRYLWAPQGNIYGSALTPKNISATRLKARSPIENLFFTGA
                     SAGYPSIGGTVGAGIRLFTELTGTKV"
     gene            complement(2128..2496)
                     /locus_tag="VMZ53_02195"
     CDS             complement(2128..2496)
                     /locus_tag="VMZ53_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27280.1"
                     /translation="MVMRAALALAVALLAFVACGGSSKPAAQQPISNTDKTGGTDSST
                     PPGHPAEGRVIQRTGSGGVIELIGDRAGAMRQANDEMSSHCGPNNYTIVQEGEESIAA
                     EDGGPRTATAWRVHYQCNGS"
     gene            complement(2490..3710)
                     /locus_tag="VMZ53_02200"
     CDS             complement(2490..3710)
                     /locus_tag="VMZ53_02200"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HUS27281.1"
                     /translation="MAKIKVKNPVVEMDGDEMTRIIWKFIKDKLILPYLEIDIKYFDL
                     GIESRDKTNDQITIDSANATKEHGVAVKCATITPDEARVKEFNLKEMWKSPNGTIRNI
                     IGGTIFREPIICKNVPRLVPGWTRPIVVGRHAFGDQYKATDFVVPGKGTLTMTYTPAD
                     GGKPVEYKVFDFPGGGVALGMYNLDEQIIGFARSCFNYGLNRGWPVYLSTKNTILKKY
                     DGRFKDLFQKVFDEEFAGKFKEKGTTYEHRLIDDMVASAMKWDGGFVWACKNYDGDVQ
                     SDSVAQGFGSLGLMTSVLLTPDGKTVEAEAAHGTVTRHYREHQKGKPTSTNPIASIFA
                     WTQGLKYRGQFDGTPDVVKFADALEKVCVETVESGKMTKDLAVLVGPNQGHLTTEAFL
                     DALDANLKAKMASW"
     gene            complement(3760..4728)
                     /locus_tag="VMZ53_02205"
     CDS             complement(3760..4728)
                     /locus_tag="VMZ53_02205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HUS27282.1"
                     /translation="MQADAGTLDSPVVGGTVVKPGVWPDAVAVLTKDGGLCTGTLLAA
                     DLVLTAGHCAAAEAVQVIVGRVDLEWFGGDVRDVKWSKAYPSWQSSFDVGIVMLEHPV
                     AAKPRAIAQGCSTRKELVRGTMLDVVGFGLTTTAGDGNNTRLHQAKIGVTDAACTSEP
                     ACETAVAPGGEFVAGGHGTDACFGDSGGPVYITTASGPALIGVVSRGIASYGEPCGDG
                     GVYVRADQVVKWIETTSGRMVERVACDRPADSGGGSGECEGGDGEGGDGDDSNGDDSN
                     GDCGASGDDGAGQGGCSAGGEAVGGAAILFVLSVLWLLSWRRARRS"
     gene            complement(4956..5654)
                     /locus_tag="VMZ53_02210"
     CDS             complement(4956..5654)
                     /locus_tag="VMZ53_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27283.1"
                     /translation="MFASRALACFMLTAASTASAQAITTVDKPPSRLGLIVSNGIGIG
                     HIGDTGPGDPVFYDYGETSIVHALVVDFGIRINRRLLLGLHGGTVSATKFVDGFSDFL
                     GTHETHYGIRTVQYGMTVELALGEHAWLAPWLGVQDSMSRATCIVSDEHTPRPPDLPC
                     EASSSSSSWEYQWDHRLGGGLGAGYDVYEHGGHRLSVVGALVSSSIKPIPGSRATNVS
                     YTSVSIGLAYRFWQ"
     gene            complement(5769..6815)
                     /locus_tag="VMZ53_02215"
     CDS             complement(5769..6815)
                     /locus_tag="VMZ53_02215"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HUS27284.1"
                     /translation="MSLAVRSTVVVAAVIASARFANADANLSTETPPPPGPQHSAIVG
                     GEDVPLGRWPDAVAIFGTDGSCTGTLIAPDLVLTAGHCANIKPTKVVANTIDYAKAGG
                     IHVNVTKTTAYPNWQAMYDVAVIELATPIQGIEPRKMATAHTYANFAESTAVHLVGFG
                     ATSADGTGANSHLKEAMTEVTDALCMGGHGCKKILAPGGEFVAGGTGTADSCFGDSGG
                     PVYLDTPNGTVVIASVSRGVDGSSTPCGGGGIYVRTDKVVDWIEETTGRTIAKDECTG
                     DDCADETGEDYAGASNPDEVGCNAGGTSGLGSLLLLASGIVISGRRRTRSKSPRGLAW
                     GESLLQQLSTRAAQ"
     gene            complement(6959..8155)
                     /locus_tag="VMZ53_02220"
     CDS             complement(6959..8155)
                     /locus_tag="VMZ53_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27285.1"
                     /translation="MRLSVLFAAFVACTPPQAQPPKQSADASAAVSELRAARFPEAER
                     ASLAVLKQDRKNSTAAAVHAVAKYDQAIEKLVAELGTVIDQGEGLKFFDHERGRAAWQ
                     AFLDELEVIDLDLEIAAADPAFSLELCIACWKHDFNRNGRIDDRDEKFFELEFDGRGG
                     ELQDGDPRRRPTYHFDVGDIYWARAMVSFQRAAVELVLAYRWSELDKLLGRGDAGAIV
                     IKLVDPGRVRHARELLINGLGFSERERQAYMAETDDDREWVPNPKQKSYAMPLEVDAK
                     LYETWGAVLGDVHRMLASQEGLSLREAATALLGDREAAALPAIYVDVGAMFREPTDIV
                     IDIDDTGHDDTKTYDHLLRGLLGHGYAQSMRASPLVARLRHMKDQVDRGEDTLERKLR
                     YLIWMN"
     gene            8268..8855
                     /locus_tag="VMZ53_02225"
     CDS             8268..8855
                     /locus_tag="VMZ53_02225"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HUS27286.1"
                     /translation="MYTKARIAGHPIHPMLIAFPVALYVATVVALLAHVGTHDPFWYR
                     VALYSNIGGVVMAIVAAVPGTIDLFTAVPARTRARATGIRHAAFNVLSLVLFAISAYM
                     LYRNAGSTLVPEDGKYGLDTTAPLVLSLLGVLSTLVAGWLGWSMTQIHHVGVKPTRFD
                     RAGIAPEDLDDLDEIPAPEPTPYATSYEAHPTLRH"
     gene            8955..10319
                     /locus_tag="VMZ53_02230"
     CDS             8955..10319
                     /locus_tag="VMZ53_02230"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HUS27287.1"
                     /translation="MQLASTIRGVRYSFGSVKEVLAKANPPKSGDDLAGVSARDAVER
                     VAARAVLADLTLADLRMNPVVPYDVDEVTRIVEDNLDAQAYRAVSHLTVGQFREWLLD
                     IETTGPTLRAVAPGLTPEMAAAAAKLMSNFDLMTVGSKAQVVVRANNTLGLPQRLSSR
                     CQPNHPTDDVQGILASMREGLSYGCGDAVIGVNPATDTPESTAEILTAIDEVLKRNAV
                     PTQHCVLSHVTVQMKALEALHCPMDLMFQSLAGSEAGNRAFGISVKLLDEAWALMKEK
                     GSTKGPNRMYFETGQGSALSSGTNHGADQVTIEARAYGLARRYKPFLVNTVVGFIGPE
                     YLEDGPQITRAALEDHFMGKLLGLPMGCDACFTYHAKMSQDELESLKVLLGSAGCTYL
                     MSLPVGDDIMLNYQSTSYHDIPTVRRLLNKRPTPEFDAWLAEKDIFHGDKPGKYFGDV
                     AAIR"
     gene            10505..11425
                     /locus_tag="VMZ53_02235"
     CDS             10505..11425
                     /locus_tag="VMZ53_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27288.1"
                     /translation="MTGTLASTGPYFATPMFFNYDVSTLPKASNSATIISSLKAAGGW
                     GNGNRMQIDFTLSVNKATSTTPKRTFTKNSDFYSPDCDTSAVPIPTGGNVEGNSGYVC
                     SNGGDCHLIVYDTSTKKLYEMYKASVTSSSFSGGCLSVWDGAKTYTDLLRGDQCTSAD
                     AAGFPIAPLLFTADEVKAGAINHAIRFILPNNRIKKGFVRPATHGTNTTGGANAPYYG
                     VHMRLRADYPINSLPTPGARVVARAMQKYGMYHADGGNIALTAASDRNTTAKWSGLLG
                     ANDLSALKVEDFEVVSHGSAVPLTMNCVRK"
     gene            11425..12498
                     /locus_tag="VMZ53_02240"
     CDS             11425..12498
                     /locus_tag="VMZ53_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27289.1"
                     /translation="MRSILGASLLVAMLGCGTDDVPQEPGQPGEDYAGPADDNGGGGD
                     DGAGSGGGQTPVPSTGPYFSTPMFFNQHVAGVAKAANSDSIIASLKAAGGWGMANRFT
                     VDFEINVLQATAATASRTFTKNALFYSPDCDDTAIPVPAGGNVEGNPGYTCTTNGDCH
                     LLVFDAAANKLYELYKASITSAAFTGGCLAVWDTSKQWTDGLRGDQCSSADAAGFPVA
                     PLMFTADEVKSGAINHAIRLVLPNDRVKKGYVRPATHGTNTTGGANAPYYGVHLRLRA
                     DYPIASLPSEGARVVARAMQEYGAYHADGGQQALTALNDRDTTAKWAGLLGAYDLDAL
                     KVEDFEVIDHGSPTAVTYACARN"
     gene            complement(12577..13554)
                     /locus_tag="VMZ53_02245"
     CDS             complement(12577..13554)
                     /locus_tag="VMZ53_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27290.1"
                     /translation="MKFPVLGAALAVALLTFPASSNARSLTDVVLDPQPGDPQPESAP
                     VVDLPLELPAPAPLPVPADKRLDELVKAGREWRIETTRGPIHVWIPASYDAATAATVV
                     FVHGYHVDIDHAWDDYHLPQQFALSGINAMFIAPEAPLGKRDAISWPSLRALVASVAT
                     HLDGVHMPTKRLVAMGHSGAYRTLAVWLANEGLDTVVLLDAVYGEYSFAKWLRESKTR
                     RLINIAYETGRYSDYMHRMLPSTTKVDGLPLGGFPDARITYAKTDVGHWQLVTDGVAI
                     PLSLRAIKVPMLDDAPMDIPLGLPQRCKPAATQETVAASDPPVPVLPPE"
     gene            13722..14543
                     /gene="eutC"
                     /locus_tag="VMZ53_02250"
     CDS             13722..14543
                     /gene="eutC"
                     /locus_tag="VMZ53_02250"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HUS27291.1"
                     /translation="MPEIPAPPARVYTPRPEKRPFAAEAARHTTALVGIGHVGTRYAT
                     DVVLQFQAELAVAHAAVGTLLPAEWAAQNGFIGVKSRVTDHREFLLRPDLGRRLDAES
                     LAIVQTQCTKNPDVQIIVADGLSAVACMGSGKALHDAVARACQARGLSVGSPICARFA
                     RVWLEDEIGQEVGAKVAMILLGERPGLGTGDGLSAYVVHDPRCGKTDGDRNMISNIHA
                     RGIPIEDAAARLASFATAMIAQRTSGVTLDLSLLGEAGERAPRAPQVRQKLVEHG"
     gene            14543..15199
                     /gene="eutL"
                     /locus_tag="VMZ53_02255"
     CDS             14543..15199
                     /gene="eutL"
                     /locus_tag="VMZ53_02255"
                     /inference="COORDINATES: protein motif:HMM:NF011934.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HUS27292.1"
                     /translation="MAILEAITPTVLSVRRIPNADPAMVRAYGGDPDKHRALGLVTVD
                     QDDPTYVALDEATKHAPVDVIFARSFYAGAAHSSGRLSGEILGVLAASEPESIEHALT
                     ALLRCLEHDACFYDADGKRTVTVFPHVIASLGEYLSKEAGLAPGDPMAYLVAPPLEAS
                     IALDAALKAADVRAVKVMTPPTETNFAAAWLTGTLDECEAAAMAYAEAVVEVAASPRR
                     "
BASE COUNT         2546 a         5011 c         4966 g         2698 t
ORIGIN      
        1 atggggcgcg tccacgcttc gtgcgggcgc tcgaccggga gaacaacttt ggggcggcgc
       61 tcttggccgc cgaacatcca ctcgcggagc atccagccgg taccgcggcg ctgaccgtcg
      121 gccgcttcag ggcgcaggct ctcgctcacg ccgctcgtgt tgtggaaggt gccgtcgcga
      181 tattgcttcg atgcgaccac acgctcgcga cgcaggcctt cgctgcgccg gccttcgacg
      241 gtgtcgggcg cgcggccttc tgcctcttcg gtcgcggcgg ttgcggccga ggcgagcgcg
      301 gcacccggag cttggggtac ctccgcgaga tccgaggccg cgggaacttg atcggggtcg
      361 tcgaacgcgt cgctcatgcg tgcgctaaag cgttacgcct tgctcgacat gccgcaagtg
      421 tcgggtttct tggcaggcaa gcgcctacgc aggcgcaagg tccttcgcag gctcagactt
      481 ttgtgccggt tagctcggtg aacaggcgga tgccggcgcc gacggtgccg ccgatcgacg
      541 ggtagcccgc gctggctccg gtgaagaaca gattctcgat cggcgagcgt gccttgagcc
      601 gcgtcgccga gatgttcttc ggcgtcagcg cggagccgta gatgttgcct tgcggtgccc
      661 agaggtagcg gcggttcgtg gtcggcgtac ccgcgacgcg catcgcgagg tgcttgcgga
      721 ggttcggcac gtagtgctcc tgcaccgtcg cgatcatgcg atcgcgaatc gcgaccttgc
      781 ggcgtgtgta ctctttgcga tcgcgcgcgc tgcgctcgtc gaacggcttg tacgcacacg
      841 tcgtgatcag ctcgaggatc tgttcgcctt ctggtgcgtg tcgtttcttc gcgttcggcg
      901 agcacagggt cggcgtcgac atgaagagcc acggcttgtc gaggtcgtcg cgctcgtgtt
      961 gggcgtcgta gaccgcgttg atgtcgaggc tcgggtagtg ccacacgttc cagttgccga
     1021 agccgtgctc gcggaggtcg attccgcgca ctccgaggta gagcgagaac gacgacgccg
     1081 agtagtcgta gttgacgcgc ttcatgtagt cgcgcgggaa gtgctgctcg ccgaggagcc
     1141 gcacgaacca tttcggatcc gcgttacaga tgatcttcgc gccgcgaaac tcgcgtccgt
     1201 ccgtcgcgac gaccgctgca acgcggccgc cctccatgcg cacgctcgcg acttcggtgt
     1261 tgtagaagat gtcgcagccc ttcttgctcg tgacggcgtt ggcgacgcct tcgatcagag
     1321 atgcgaagtg ctccgtcggg tagtacgcgc cggcgcagta cttcgagatc agcgccgtgt
     1381 agaggagcag cgagatgtcg cgcggcggta ggccggcatc gccgagctgc gtcgacagga
     1441 tcgcctgcac gggcgcagac aggcgcagct tgtcgaacag gttctgcagc gtgctcgccc
     1501 gatggagcgc gacgtgcggc gcggcgatga ggcctttcaa gatgcgaagc ccggtgagat
     1561 tttcgggcag ccgcccgagc tcggtcgcga gcgttttgat cgtcgagaag aacgccgtga
     1621 tcgcctttgt ctcggcgggg aactgctcgg agaggcgcgt gcaccacgcg tcgaggccgc
     1681 tcgggatgcg aaacgtcagg cccgccgacg ggcagcgaaa gtgatcgtag ccgtcggggt
     1741 tgaggcgctc gaacgtgatc gtttcgtgga ggccgagcgc gcggagcaag ttgtgaacgg
     1801 gctcgccttc gccgcacgag aagatgtagt ggaccgcggc gcaaaaccga tagtcgccca
     1861 tcgggaacgt gtggcagcag ccgccgggat actcgtgcgc ctcgacgatc gcgacgcgtg
     1921 cgccagcgtt ggcgagcagc gcaccgacgg tgagcccgcc catccctgcg ccgacgatca
     1981 cgtagtcgta atgactcgct ggagttgtcg ccgacatctg cgacgacgtc gtcgaagaag
     2041 tctgcgatga catcgccgat ggcgtcgtgg atggcatgct cgatgtcgtc gattgcatgc
     2101 gtcggcgtgg gtaccggcgc agttaactta gctaccgttg cactggtagt ggacgcgcca
     2161 cgcagtcgcg gttcgcgggc cgccgtcctc cgccgcgatc gactcttcgc cttcttggac
     2221 gatcgtgtag ttgttcggcc cgcagtggga cgacatctcg tcgttcgcct gccgcatcgc
     2281 gcccgctcga tcgccgatga gctcgatgac gccaccgctg ccggttcgtt gaatcacgcg
     2341 accttcggcg gggtgacctg gcggcgtcga agaatcggtg ccgcctgtct tgtccgtatt
     2401 cgagatcggc tgctgcgctg ccggcttgct cgatccaccg cacgcgacga acgcgaggag
     2461 cgcaaccgca agcgcgagcg cagcgcgcat caccacgaag ccatcttggc tttgaggttt
     2521 gcgtcgagcg cgtcgaggaa cgcctcggtc gtgaggtggc cttggttcgg gccgacgagc
     2581 accgcgaggt ccttcgtcat cttccccgac tcgaccgtct cgacgcacac cttctcgagt
     2641 gcgtcagcga acttcacgac gtccggcgtg ccgtcgaatt ggccgcggta cttgaggccc
     2701 tgcgtccacg caaagatcga cgcgatcggg ttcgtcgacg tcggcttacc cttctgatgc
     2761 tcgcgatagt ggcgcgtgac cgtgccgtgc gcggcttccg cttcgacagt cttgccatcg
     2821 ggcgtcagca gcaccgacgt catgaggccg agcgagccga agccctgcgc gaccgagtcc
     2881 gactgcacgt cgccgtcgta gttcttgcag gcccacacga agccgccgtc ccacttcatg
     2941 gccgacgcga ccatgtcgtc gatgaggcgg tgctcgtacg tcgtgccctt ctccttgaac
     3001 ttgccggcga actcttcgtc gaagaccttc tggaagaggt ccttgaaccg accgtcgtac
     3061 ttcttgagga tggtgttctt cgtcgagagg tacaccggcc aaccgcggtt caagccgtag
     3121 ttgaagcagc tgcgcgcgaa gccgatgatc tgctcgtcga ggttgtacat gccgagcgcg
     3181 acgccgccgc ccgggaagtc gaagaccttg tactcgacgg gcttgccgcc atccgcaggc
     3241 gtgtacgtca tcgtcagcgt gcccttgccc ggcacgacga agtccgtcgc cttgtactga
     3301 tcgccaaacg catgacgacc gacgacgatc ggtcgcgtcc accccggcac gaggcgcggg
     3361 acgttcttgc agatgatcgg ctcgcggaag atggttccac cgatgatgtt gcggatggtg
     3421 ccgttcggcg acttccacat ctctttcagg ttgaactctt tgacgcgcgc ctcgtcgggc
     3481 gtgatcgtcg cgcacttgac cgcgacgccg tgctctttcg tcgcgttcgc cgaatcgatc
     3541 gtgatttgat cattcgtctt gtcgcggctc tcgatcccga gatcgaagta tttgatgtcg
     3601 atctcgaggt acgggaggat cagcttgtcc ttgatgaact tccagatgat gcgcgtcatc
     3661 tcgtcgccgt ccatctcgac gacgggattc ttcaccttga tcttggccat gcggggcgtg
     3721 ttgtaacacg cgtcgtcggc gacgcagcag cgctgcgggt cacgatcgtc gagcgcggcg
     3781 ccagctcaga agccacaaga cggatagcac gaacaggatt gccgctccgc cgacagcctc
     3841 gccacctgcg ctgcagccgc cctgccccgc gccgtcgtcg ccgcttgctc cgcaatcgcc
     3901 gttgctgtcg tcgccgttgc tgtcgtcgcc gtcgccgccc tcgccgtcac cgccctcgca
     3961 ttcgccgctg ccgccacccg agtccgccgg gcgatcgcac gcgacgcgct cgaccatccg
     4021 gccggacgtc gtctcgatcc acttcacgac ctggtccgcg cgcacgtaca caccgccgtc
     4081 gccacacggc tcgccgtagc tggcaattcc gcgcgacacg acgccgatca acgccggccc
     4141 actcgcggtt gtgatgtaga ccggcccacc cgaatcgccg aagcacgcat cggtgccgtg
     4201 gcccccagcg acgaactcgc caccaggagc cacggcggtc tcacacgcag gctcgctcgt
     4261 gcatgccgcg tccgtcacgc cgatcttggc ttgatgcaga cgcgtgttgt tgccgtcgcc
     4321 ggcggtcgtg gtgaggccga agccgacgac gtcgagcatg gttcccctga cgagctcctt
     4381 gcgtgtggaa catccctgcg caatcgcgcg cggtttcgcg gcgaccggat gttcgagcat
     4441 cacgatccca acgtcgaagc tgctctgcca gctcggatac gccttcgacc acttcacatc
     4501 ccgcacgtcg ccgccgaacc actcgaggtc gacgcgacct acgatgactt gcaccgcctc
     4561 cgccgccgcg cagtgcccgg ccgtaaggac gaggtccgcc gccaacaacg tgccggtaca
     4621 caagccgccg tccttcgtca gcaccgcgac cgcatcgggc cacacgcctg gcttgacgac
     4681 ggtgccaccg acaacgggcg agtcgagcgt tccggcgtcc gcctgcatcg gcggcccgag
     4741 cgtggcgaag gaaagagaaa cgagaaggga gctaacgaaa gtgatgttgc gaagcatggc
     4801 ggcgctgcat cagcaaccac cgcgccagca ctgctcgcac gcgattctca cgcgttacgc
     4861 gccacccgca cggcgattcg tccgcctacc ggacaaaccg tccgcccgtc gtcggacacc
     4921 ggacaaagcc tttcgctccg ccaacgagat ccacgctatt gccagaagcg atacgcgaga
     4981 ccgatcgaaa cgctcgtgta gctgacattg gtcgcgcgtg accccggaat cggcttgatc
     5041 gacgagctga ctaacgcgcc gacgacgctc agacgatggc caccgtgctc gtagacgtcg
     5101 tagcccgcgc cgagtccgcc acccaatcgg tgatcccact ggtactccca cgagctcgag
     5161 ctcgagctgg cttcgcacgg aagatcgggc ggacgtggag tgtgctcgtc gctcacgatg
     5221 catgttgcgc ggctcatcga gtcctgcacc ccgagccatg gcgcgagcca cgcgtgttcg
     5281 ccaagcgcga gctcgacggt catgccgtac tgcacggtgc gaatgccgta gtgcgtctcg
     5341 tgggttccca ggaagtcgga gaaaccgtcg acgaacttcg tcgctgaaac ggtgccgcca
     5401 tgcagaccga gcaagaggcg gcggttgatt cgaatgccga agtcgacgac cagcgcgtgc
     5461 acgatgctcg tctcgccgta atcgtagaag acgggatcgc cggggccggt gtcaccgatg
     5521 tgcccgatgc cgattccatt gctgacgatg aggccgagtc gcgatggcgg tttgtcgacg
     5581 gtcgttatcg cttgcgcaga agctgtactt gcggcggtca acatgaagca cgccagtgct
     5641 cggctcgcga acatgccgcg cgccttatca agtggcgtgc ccgacgcgat ttccgaacgt
     5701 tggcgacgaa accggagcga tcgtccgaca tcactgtcag tgtcgaacgc gagatcctca
     5761 gcttttcgct actgcgcggc gcgcgtcgag agctgttgga gcagcgactc gccccaggcc
     5821 aggccgcgcg gcgacttgga ccgcgtacgg cggcggcccg agatcacgat gccggatgcg
     5881 aggagcagga ggctgccgag gccggacgtg ccgcccgcgt tgcagccgac ttcgtcgggg
     5941 ttggacgcgc ccgcgtagtc ttcgccggtc tcgtcggcgc agtcgtcacc ggtgcactcg
     6001 tctttcgcga tcgtgcggcc ggtcgtttct tcgatccagt cgacgacctt gtcggtgcgc
     6061 acgtagatgc caccgccgcc gcaaggcgtg ctggagccgt cgacgccgcg cgacaccgat
     6121 gcgatgacga ccgtgccgtt cggcgtgtcg aggtagaccg ggccgcccga gtcgccgaag
     6181 caggagtcgg ccgtgccggt gccgccggcg acgaattcgc cgcctggagc caggatcttc
     6241 ttgcagccgt gaccgcccat gcagagggcg tcggtgactt ccgtcattgc ctctttgagg
     6301 tgcgagttgg cgccggtgcc gtcggccgac gtcgcgccga agccgacgag gtggactgcc
     6361 gtggactcgg cgaagttggc gtacgtgtga gcagtcgcca tcttgcgcgg ctcgatgcct
     6421 tggatcggcg tggcgagctc gatgacggcg acgtcgtaca tcgcttgcca gttgggatac
     6481 gcggtcgtct tcgtcacgtt gacgtggatg ccgcccgctt tggcgtagtc gatcgtgttc
     6541 gcgacgacct tcgtcggctt gatgttcgcg cagtgtcctg cggtgaggac gaggtcgggc
     6601 gcgatcagcg tgcccgtgca cgaaccatcg gtgccgaaga tcgcgacggc gtcgggccag
     6661 cgaccgagcg ggacatcttc gccgccgacg atcgcagagt gctgcgggcc cggcggtggc
     6721 ggcgtctccg tcgacaggtt cgcgtcggcg ttcgcaaacc tagcagacgc gatgaccgcg
     6781 gccaccacga ctgtcgagcg caccgcaagc gacatgcggg cgacaatgag caacgtatgt
     6841 gcccgtcgga gatcgtgcgc tcctgcgcat caggcagcgg cgcgaatgtc gcgtcggcgc
     6901 acgcacgcgt tgcggcatca cgaggttgcg cgtgtccgag ctgcgagctt ttgcgcggtc
     6961 agttcatcca gatcagatat cggagcttgc gctcgagggt gtcctctcct cgatctacct
     7021 ggtccttcat gtgacggagg cgcgcgacga gcggcgaggc gcgcatcgat tgtgcgtagc
     7081 cgtggccaag cagaccgcga agcaagtgat cgtacgtttt cgtatcgtcg tgcccggtgt
     7141 cgtcgatatc gatcacgatg tccgtgggct cgcggaacat cgcgccaacg tcgacgtaga
     7201 tggccggcag tgcggctgcc tcgcggtcgc ccaggagcgc cgtggcggct tctcggagcg
     7261 ataggccctc ttgcgacgcg agcatgcggt gtacgtcgcc gaggaccgcg ccccacgtct
     7321 cgtagagctt cgcatcgacc tcgagcggca tcgcgtagct cttctgtttc gggttcggca
     7381 cccattcgcg gtcgtcatcg gtttcggcca tgtacgcctg acgctctcgc tcggagaagc
     7441 cgagtccatt aatgaggagc tcgcgcgcgt ggcgaacgcg gcctggatcg accagcttga
     7501 tcacgatcgc tcctgcgtcg ccgcgaccga gcagcttgtc gagctccgac cagcgatatg
     7561 cgaggacgag ctcgaccgct gcacgctgaa acgacaccat cgcgcgcgcc caatagatgt
     7621 cgccgacgtc aaagtgatac gtcgggcggc gcctcggatc gccgtcttgc agctcgccac
     7681 ctcggccgtc gaattcgagc tcgaagaatt tctcgtcgcg gtcgtcgatg cgtccgttgc
     7741 gattgaagtc gtgcttccag cacgcgatgc acagctcgag gctgaacgcc gggtctgccg
     7801 ccgcgatctc gagatcgagg tcgatgactt cgagctcgtc gaggaacgcc tgccatgcgg
     7861 cgcgaccccg ttcgtgatcg aagaacttga ggccctctcc ttgatcgatc accgtgccga
     7921 gctcggcgac cagcttctcg atcgcctggt cgtattttgc gactgcgtgg accgcggcgg
     7981 ccgtcgaatt tttccgatct tgtttgagca ccgcgagcga agcgcgttcg gcctcgggaa
     8041 atcgcgctgc gcgcaattca ctcactgcgg cgctagcgtc ggcgctctgt ttcggcggct
     8101 gcgcctgtgg cggcgtgcac gcgacgaatg ctgcgaacaa gacggagagt cgcatggcgc
     8161 aatactgcga gagcttcgcg cgcattccac tcagatcacg gctttttcac gcgttggact
     8221 ttgccggcat acggactgca attgccagtc gtcgaggaga aggcgtcatg tacacgaaag
     8281 cgcgcattgc agggcatccc attcacccga tgctgattgc gttccctgtc gcactgtatg
     8341 tcgcgaccgt ggtcgctctc ttggcccacg tcggtacgca cgacccgttt tggtatcgcg
     8401 tcgcgctgta ctcgaacatc ggcggcgtcg tgatggcgat cgtcgcggcg gttccaggca
     8461 cgatcgacct cttcacggcg gtgcctgcac gcacccgcgc tcgagcgacc ggcatccgcc
     8521 atgcggcatt caacgtcctg tcgctcgtgc tgttcgcgat cagcgcgtac atgctgtatc
     8581 gcaacgcggg cagcacgctg gtccccgagg acggcaagta cgggctcgat acgaccgcac
     8641 cgttggtcct ctcgctgctc ggtgtccttt cgacgctcgt tgcgggctgg cttggttggt
     8701 ctatgacgca gatccatcac gtcggcgtga agcccacccg cttcgaccgc gccggcatcg
     8761 cgcccgagga cctcgatgac ctcgacgaga ttcccgcgcc ggaaccgacc ccgtacgcca
     8821 cctcgtacga ggcccatccg acgcttcgcc actagtttca gactgcgact tagttagctt
     8881 gttcccgccg ccgtcgtgtc tggtcattgg atccgaacga cgcgcggcgt tttcttttgt
     8941 acgatgcgcc ggcagtgcag ctcgcatcga cgattcgcgg cgttcgctac agcttcggct
     9001 ccgtgaagga agtcctcgcg aaggcgaacc cgccgaagag cggtgatgac ctcgccggcg
     9061 tgtcggcgcg cgatgcagtc gagcgtgtcg cggcgcgtgc ggtgctcgcg gatctgacgc
     9121 tcgccgatct gcggatgaat cccgtcgtcc cgtacgacgt cgacgaggtc acgcgcatcg
     9181 tcgaggacaa cctcgatgcc caggcgtatc gcgcggtcag tcacctcacc gtcgggcagt
     9241 tccgcgagtg gttgctcgat atcgagacga ccggaccgac gctgcgcgcg gtggcaccgg
     9301 gcctcacgcc ggagatggcg gccgctgcag cgaagctgat gtcgaacttc gatctgatga
     9361 ccgtcggctc gaaggctcag gtcgtcgtcc gcgcgaacaa caccctcggc ctgccgcagc
     9421 gcctcagctc gcggtgccag ccgaaccatc cgacagatga cgtgcaaggc atcctcgcgt
     9481 cgatgcgcga agggctgtct tatggctgcg gcgatgcagt catcggcgtg aatcccgcga
     9541 ccgacacgcc cgagtcgaca gcggagatcc tcacggcgat cgacgaggtg ttgaagcgca
     9601 acgcggtccc cacgcaacac tgcgtgcttt cacacgtgac cgtgcagatg aaggcgctcg
     9661 aagcgctgca ctgcccgatg gatctcatgt tccagtcgct cgccggctcc gaggcgggca
     9721 atcgcgcatt cggcatcagc gtcaagctgc tcgacgaagc gtgggcactg atgaaagaga
     9781 agggctcgac gaaaggcccg aaccgcatgt acttcgagac cggccaaggc tcggcgctct
     9841 cgagcggaac gaatcacgga gctgaccaag tgacgatcga agcgcgcgcg tacgggctcg
     9901 ctcgccgcta caagccgttc ctcgtcaaca ccgtcgtcgg tttcatcggt cccgagtatc
     9961 tcgaggatgg cccgcagatc acgcgcgctg cgctcgagga ccacttcatg ggcaagctgc
    10021 tcgggctgcc gatgggctgc gacgcgtgct tcacctatca cgcgaagatg agccaggacg
    10081 agctcgagag tctcaaggtc ctgctcggct ctgcgggctg cacctatctg atgtcactgc
    10141 cggtcggcga cgacatcatg ctgaactacc aatccacgag ttatcacgac atcccgaccg
    10201 tccgacgctt gctgaataag cggccgacgc cggaattcga cgcgtggctc gctgaaaaag
    10261 acatctttca cggcgacaag cccggcaagt atttcggcga cgttgcagcg atccgctgag
    10321 agctcttccc actcttagcg cactgcacaa gggtggactc cgattgcgcc cgacgcgcat
    10381 ggccgcacag tgtgcgcgaa tgaaaaattt cctgggggta gcgattcttc tgtctgcagt
    10441 ggcgacgggg tgcgtggacg gtgacgacgg attggaagaa aacctatcgg atacggatca
    10501 agcggtgacg ggtacgctcg cgtcgacggg cccgtacttc gcgacgccga tgttcttcaa
    10561 ctacgacgtc agcacgctgc caaaggcgtc gaacagcgcg acgatcattt cgtcactcaa
    10621 ggcagccggc ggctggggca acggcaaccg catgcagatc gacttcacgc tcagcgtgaa
    10681 caaggcgacg tcgacgacgc cgaagcgcac gttcacgaaa aacagcgact tctactcgcc
    10741 cgactgcgac acgtcggcgg tgccgattcc gacgggtggc aacgtcgaag gcaacagcgg
    10801 ctatgtctgc agcaacggcg gcgactgcca cctcatcgtc tacgacacca gcacgaagaa
    10861 gctgtacgag atgtacaagg cgtcggtgac gtcgtcgtcg ttcagcggcg gctgcctctc
    10921 ggtctgggac ggcgcgaaga cgtacacgga tctccttcgc ggcgatcagt gcacgagcgc
    10981 ggacgcggca ggcttcccga tcgctccgct cctcttcacc gccgacgaag tgaaggcagg
    11041 cgcgatcaat cacgcgattc gcttcatctt gccgaacaac cgcatcaaga agggcttcgt
    11101 tcgtcccgcg acgcacggca cgaatacaac aggcggcgcg aacgctccgt actacggcgt
    11161 ccacatgcgc ctgcgcgccg actatccgat caactcgctg ccgacgccgg gtgcacgcgt
    11221 ggtcgctcgc gcgatgcaga agtacggcat gtatcacgcg gacggcggca acatcgcgct
    11281 gaccgcggcg agtgatcgca acacgacggc gaagtggagc ggcctgctcg gcgcgaatga
    11341 tctgtcggcg ctcaaggtcg aagacttcga agtcgtgagc cacggctctg cagttcctct
    11401 cacgatgaac tgcgtgcgca agtaatgcga agcattcttg gcgcgtcgct cctcgtcgca
    11461 atgctcggtt gtggaaccga tgacgttccg caggaaccgg ggcagcccgg cgaggactac
    11521 gcgggtccag cagatgacaa cgggggtggt ggagacgacg gcgcgggctc tggtgggggc
    11581 cagacgcctg tgccgtcgac gggaccgtat ttctcgacgc cgatgttctt caatcaacac
    11641 gtcgccggcg tcgcgaaggc cgcgaacagc gactcgatca tcgcgtcgct caaagccgcg
    11701 ggtggttggg gcatggcgaa ccggttcacg gtcgacttcg agatcaacgt gctgcaagca
    11761 acagcggcga cggcgtcgcg cacgttcacg aagaacgcac tgttctactc gccggattgt
    11821 gatgacacgg cgattcccgt gcccgccggc ggcaacgtcg aaggcaatcc cggctacacg
    11881 tgcacgacga acggcgactg ccacctgctc gtgttcgacg ccgccgcgaa caagctctac
    11941 gagctctaca aggcgtccat cacgtcggcc gcgttcaccg gcggctgcct agcagtgtgg
    12001 gacacatcga agcagtggac ggacggccta cgcggcgacc agtgctcgag cgctgacgct
    12061 gcgggcttcc ccgtcgcgcc gctgatgttc accgccgacg aagtgaagtc cggcgcgatc
    12121 aaccatgcga tccgcctcgt gctgccaaac gatcgcgtga agaagggcta cgtccggccg
    12181 gcgacgcacg gcacgaacac gacgggcggc gcgaacgctc cgtattacgg cgtgcacctt
    12241 cgccttcgcg cggactatcc gatcgcatcg ctgccctccg agggcgcacg cgtcgtcgca
    12301 cgcgcgatgc aggagtacgg cgcgtatcac gccgacggcg gccagcaggc gctcacggcg
    12361 ctcaacgatc gcgacacgac tgcaaagtgg gcgggcctgc tcggcgcgta cgacctcgac
    12421 gcgctgaaag tcgaagactt cgaagtgatc gatcacggct cgccgaccgc ggtcacgtac
    12481 gcctgcgccc ggaactaggg tcaggcacaa gaaaacaaga aactcacgat cgtccctgcg
    12541 gagttacgcg cgtttcttgt gaacttgtgc ctgaccctac tcgggcggca gtacggggac
    12601 cggcgggtcg ctcgcggcga ccgtctcttg cgtcgcggcc ggcttgcagc gttgcggcag
    12661 gccgagcggg atatccatcg gcgcgtcgtc gagcatcggc actttgatcg cgcgcagcga
    12721 gagcgggatc gcgacgccgt cggtgacgag ctgccagtga ccgacgtctg tcttcgcgta
    12781 cgtgatgcgc gcgtcgggga agccgccgag cggcaggccg tcgaccttcg tcgtgctcgg
    12841 cagcatgcga tgcatgtagt ccgagtagcg gccagtctcg tacgcgatgt tgatgaggcg
    12901 gcgcgtcttg gattcacgca gccacttcgc aaagctgtac tcgccgtaga cagcgtcgag
    12961 gagcacgacc gtgtcgaggc cttcgtttgc gagccacacg gcgagcgtgc ggtaggcacc
    13021 ggagtggccc atcgcgacga gccgcttcgt cggcatgtga acgccgtcga gatgcgtggc
    13081 gacactcgcg acgagcgcgc gcaacgacgg ccacgagatg gcgtcgcgtt tgccgagcgg
    13141 cgcttccggc gcgatgaaca tcgcgttgat gccagagagc gcgaactgct gcgggagatg
    13201 gtagtcgtcc cacgcgtgat cgatatcgac gtgatagccg tgcacgaaca cgacggtcgc
    13261 tgcggtcgcc gcgtcgtagc tcgccgggat ccagacatgg atcgggccgc gcgtggtttc
    13321 gatgcgccac tcgcgtccgg ctttgacgag ctcgtcgagg cgcttgtctg cgggcaccgg
    13381 cagcggcgcg ggtgcgggca gctcgagcgg caggtcgacc accggtgccg actccggctg
    13441 cggatcgccg ggctgtggat cgaggaccac gtccgtcaat gaacgtgcgt tcgaggaggc
    13501 cgggaatgtc agcagcgcga cagcgagtgc tgcgccgagg acagggaact tcacacttca
    13561 agtttccctg gggagcgtcg ggaaggccag gaaaaatcgg ctggcttgcc gtcgggctcg
    13621 tgtctgtggt cacgtagggc tccgtgagtc gtacccggca actcgtcgcc gcattgcgcg
    13681 atgcgcttgg cagcgcagaa ccgctgcctc ccccgccgcc tatgccggag atccccgctc
    13741 cgcccgcacg ggtgtacacg ccgcgcccgg aaaagcggcc gttcgccgcc gaggcagcgc
    13801 gccacaccac ggctctcgtc ggtatcggcc atgtcggcac gcgctatgcc accgacgtcg
    13861 tcctccaatt ccaggccgag ctcgccgtcg cccacgccgc ggtcggcacg ctcctgccgg
    13921 ccgaatgggc agcccagaac gggttcatcg gcgtcaagtc ccgggtcacc gaccaccgcg
    13981 agttcctact ccgccccgac ctcggccgcc gcctcgacgc cgagtcgctc gcgatcgtcc
    14041 agacgcagtg caccaagaac ccggacgtcc agatcatcgt cgcggacggc ctctccgccg
    14101 tcgcctgcat gggctcgggc aaggcgctcc atgacgcggt cgctcgcgcg tgccaagccc
    14161 gcggcctctc ggtcggatcg cccatctgcg cccggttcgc gcgggtctgg ctcgaagacg
    14221 aaatcggcca agaggtcggc gccaaagtcg cgatgatcct gctcggcgaa cgccccggcc
    14281 tcggcaccgg cgacggcctc tcggcctacg tcgtccacga ccctcgctgc ggcaaaaccg
    14341 acggcgaccg caacatgatc tccaacatcc acgcccgcgg cattccgatc gaagacgccg
    14401 cggcccggct cgcgtcgttc gccaccgcga tgatcgcgca acgcacgagc ggcgtgacgc
    14461 tcgacctctc cctcctcggt gaagcaggcg aacgcgcccc tcgcgcaccg caagttcggc
    14521 agaaactggt ggagcacggc tgatggcgat cctcgaagca atcactccga ccgtgctctc
    14581 ggtgcgtcgc atcccgaacg ccgatccggc gatggtgcgc gcatacggcg gcgatcccga
    14641 caagcaccgc gcgctgggcc tcgtcacggt cgatcaagac gacccgacgt acgtagcgct
    14701 cgacgaagcc acgaagcacg cgcccgtcga cgtgatcttc gcgcgctcgt tctacgcagg
    14761 cgccgcgcac tccagtggcc gcctctccgg cgagatcctc ggcgtgctgg ccgcgtcgga
    14821 gcccgagtcg atcgagcatg cgctcaccgc gctcctccgc tgcctcgagc acgacgcctg
    14881 cttctacgac gccgacggca agcgcacggt cacggtgttc ccgcacgtga ttgcatcgct
    14941 cggcgaatat ctctcgaagg aagcgggcct cgcgcccggc gatccgatgg cgtacctcgt
    15001 cgcgccgccg ctcgaggcgt cgatagcgct ggatgccgcg ctgaaggccg ccgatgtgcg
    15061 cgcggtcaaa gtgatgacgc caccgacgga gacgaacttc gcagcggcgt ggctgaccgg
    15121 tacactcgac gagtgcgaag cagcagccat ggcgtacgcc gaggcggtcg tcgaagtggc
    15181 tgcgtcgccg cggcgctagt gctcctcgcc gcgtgcggcg a
//
LOCUS       DATLBZ010000046         5348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1527744, whole genome shotgun sequence.
ACCESSION   DATLBZ010000046 DATLBZ010000000
VERSION     DATLBZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527744"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VMZ53_02260"
     CDS             <1..265
                     /locus_tag="VMZ53_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUS27293.1"
                     /translation="EPTTGLHPADVEKLVGQLDALVEAGNTVVVVEHEMRVIAASDWV
                     IDIGPGAGDEGGHIVAEGPPAVVAKAKDSRTAPYLARFLDYAR"
     gene            269..1126
                     /locus_tag="VMZ53_02265"
     CDS             269..1126
                     /locus_tag="VMZ53_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02996 domain-containing protein"
                     /protein_id="HUS27294.1"
                     /translation="MSEELLAKVLAKPADDAARAAYAAALHKAGDPRGEFIDLQCDLA
                     KHKDHKTPAAKKLAKRIAELHKKHAAKWAKPLRALGDGTKWELKRGFVERLEVVGEPR
                     MAALNDVFAIEPVTDLITGGRTAWLKSLFASAGMWRVERLVLQIDFGQAKAVIAELTR
                     ATLPNLVELRMGVLKDQHLEGLGEVAPPKLVHLCVGGVGITEKGVGKLLDSPLGRKLE
                     TLELDRFKIDPRMANLICATQIKRFSASDGWVDEVEDQLKARFGKNFVVEDEESFDYL
                     LYGTKGISR"
     gene            1192..1515
                     /locus_tag="VMZ53_02270"
     CDS             1192..1515
                     /locus_tag="VMZ53_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27295.1"
                     /translation="MRIEREQAGVTEVSFGRGAQNAPFVVALWRRERFIFWPAAIATA
                     AALHFTALRWPLAYIEIPGAVGFTLGGFLSVVRAGRSAPERAITSAWWAATIACAAAL
                     AVLLR"
     gene            complement(1504..2010)
                     /locus_tag="VMZ53_02275"
     CDS             complement(1504..2010)
                     /locus_tag="VMZ53_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27296.1"
                     /translation="MKKLSLVLLAVLSLAAIGCKSKAGAGDCGAAVNHSMDLSKADMK
                     KMGTDDKTMAKMVEVGVTRCKEDKWSADAIKCMTNAATMSDAQGCYSKLTADQQKKMN
                     EAAMAVVMPSAPTGTGDGSAPAGDTGSATGDMAAGSAAAGSAAAGSAAAGSAAAGSAE
                     AGSAGSAK"
     gene            2099..2707
                     /locus_tag="VMZ53_02280"
     CDS             2099..2707
                     /locus_tag="VMZ53_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27297.1"
                     /translation="MFALLAAGGLIVGCFGPKWLVSVRGPRMAIGLRNFQVCAGGQCE
                     AISNFQMVDELDKQIAKLKETYADAPLGVQRQAIPENPWHGFPVVGWITLAMGLISAI
                     GLVWGAVLALQRKRPDLPIMPTTLAVIGLALGIVNGCLVLATKPDIIDDIAVGWSFWA
                     FGAGAVLGLTAVFPLNRQIRPIDEELGEASATMSWGASRDDE"
     gene            complement(2708..3007)
                     /locus_tag="VMZ53_02285"
     CDS             complement(2708..3007)
                     /locus_tag="VMZ53_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27298.1"
                     /translation="MQTEIIELSKLAAPKRAAFGEFLVMARVLDRFQLFRALQLQDRM
                     PGERLGQCAVALGYVARMQIEHMHAAFASVVRDAELETMETEAFEPVVEEIVLEP"
     gene            complement(3059..3502)
                     /locus_tag="VMZ53_02290"
     CDS             complement(3059..3502)
                     /locus_tag="VMZ53_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27299.1"
                     /translation="MGALRNSFVLLVLATATASADPPQRPTLVLSGFSFSDPTPQPPP
                     VSLRIEAARVAAERFEDNWRYPEPGAMVGVDGNGWFIGAGHYRPRTARSGALHGGSIA
                     ATMAGEILMGTGSPLAGVGALLAGATLDAAAADVDRDAESRHPSN"
     gene            3592..4116
                     /locus_tag="VMZ53_02295"
     CDS             3592..4116
                     /locus_tag="VMZ53_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS27300.1"
                     /translation="MTWTIRPVDRNADRRAVSAIDTSFETTSIYEVVATARSLELVER
                     PATVTKRYPMADAFAPWSTWDTGWVAHSGDEALGFAGVEFEAWHARLVLWHLYVTRSH
                     RRTGIGRALLAEVESYGRSLGARRVWLETTSVNVPGIAAYARLGYSLVGLDTSVYDTL
                     PYSNEAAIYLAKSL"
     gene            complement(4122..4301)
                     /locus_tag="VMZ53_02300"
     CDS             complement(4122..4301)
                     /locus_tag="VMZ53_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27301.1"
                     /translation="MRTPPGFDEMLIDQQVLHFFRALALKDKLRVRFAANGEQLDLET
                     LQVEAFVSEPERVAS"
     gene            complement(4385..>5348)
                     /locus_tag="VMZ53_02305"
     CDS             complement(4385..>5348)
                     /locus_tag="VMZ53_02305"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27302.1"
                     /translation="LQRGLVDWEPLALALGDQAGSGMRLCSLLVSRGLLDFDEASRLL
                     GEVHHSAAALRRHLEGRDESLADLLPDDIAKQLVALPIGRLANGTLIICVREPSAALQ
                     AKLTRIVREEVVLAIAPALYLERIVEAAYAPRELDSSELEEELQEVPQVVEDFDIPID
                     VEELPKPKKKQRALSVVIPTLAVTSERDALDATLASFREIDDAEWLFDVAMKYISTHW
                     TSSLLLVVREQRAIGIRGHGARISPNLVKTLAVDIADLGDAAAPGSEYEVTTKALGVM
                     HPVVTPLRGSHILLLGDPIGKDRDDALVDLGLLVESMNDALARM"
BASE COUNT          923 a         1726 c         1734 g          965 t
ORIGIN      
        1 cgagccgacg acaggcctgc accccgccga tgtcgagaag ctcgtcggtc agctcgatgc
       61 gctcgtcgag gctggcaata cggtcgtcgt cgtcgagcac gagatgcgcg tgatcgcagc
      121 gagcgattgg gtgatcgata tcggcccggg tgcaggtgat gaaggcggcc acatcgtcgc
      181 cgagggtccg cccgcggtcg tcgcgaaagc caaggacagc cgcactgcgc cctatctcgc
      241 tcgcttcctc gattatgctc gttgatcgat gagcgaagag ttactagcga aggtgttggc
      301 gaaaccggcc gatgacgcgg cgcgagccgc gtatgcagca gcgctccaca aagccggcga
      361 tccgcgcggg gaattcatcg atctgcagtg cgacctcgcg aagcacaagg atcacaagac
      421 gccggccgcc aagaaactcg cgaagcgcat agccgagctt cacaagaagc acgctgcgaa
      481 atgggcgaag ccgctgcgcg cgctcggtga cggtacgaag tgggagctca aacgggggtt
      541 cgtcgagcgt ctcgaggtcg tgggcgagcc gcgaatggca gcgttgaatg acgtgttcgc
      601 gatcgagccg gtgaccgatt tgatcaccgg tggtcgaaca gcgtggctca agtcgttgtt
      661 cgcgagcgcc gggatgtggc gcgtggagcg gctcgttctg cagatcgatt tcggtcaagc
      721 caaggccgtc atcgcggagc tcacgcgcgc aacgctcccc aacctcgtcg agcttcggat
      781 gggggtgctg aaggatcagc acctcgaggg tcttggggag gtcgcaccgc ccaagctcgt
      841 ccatctgtgc gttggcgggg tgggaatcac cgagaaaggc gtcggcaagc tgcttgactc
      901 gccgctcggc cgcaagctcg agacgctcga gttggatcga ttcaagatcg atccacggat
      961 ggctaatctc atctgcgcga cgcagatcaa gcggttctcc gcctccgacg gttgggtcga
     1021 cgaagtcgaa gaccagctca aggcgcggtt cggcaagaac ttcgtcgtcg aggacgagga
     1081 aagcttcgac tatttgctct acggcacgaa gggaatcagc cgctgagcgt cgcgcagatc
     1141 ctcgtcggtg ccctgctgtt tctcgtcgct gcgaaggcgc atggcaggca gttgcgcatc
     1201 gagcgcgagc aagcgggcgt caccgaagtc tcgttcgggc gaggcgcgca gaatgcgccg
     1261 tttgtcgtcg ccctttggag acgcgagcgc ttcatcttct ggccggctgc gatcgcaacg
     1321 gcagccgcgc tgcacttcac cgcgctgcgc tggcctctcg catacatcga gattcccggc
     1381 gcggttggct tcacgctcgg cgggtttctc agcgtcgtgc gcgcgggacg gtcggcaccg
     1441 gagcgcgcga taacgagcgc gtggtgggcc gcgacgatcg catgcgcggc agctctcgcg
     1501 gttctacttc gctgagccgg cggagcctgc ttcggcgcta cccgccgctg cgctaccggc
     1561 tgccgcgcta cccgccgccg cgctccccgc cgccgcgctt cccgccgcca tgtcgccggt
     1621 ggccgagccc gtgtcgccag ccggcgccga gccatcgccc gtacccgtcg gcgccgacgg
     1681 catcacaacg gccatcgccg cctcgttcat cttcttctgc tggtccgcag tgagcttgct
     1741 gtagcaacct tgcgcgtcgc tcatcgtcgc tgcgttcgtc atgcacttga tcgcgtcggc
     1801 cgaccacttg tcttctttgc accgcgtgac gccgacctcg accatcttcg ccatggtctt
     1861 gtcgtcggtg cccatcttct tcatgtcggc cttcgacagg tccatgctgt gattgacggc
     1921 cgcgccgcag tcgccggccc cggcctttga cttgcagcca attgcggcga gagagagcac
     1981 ggcgaggaga acgagcgaga gctttttcat ccccccgacg ttcgaaattt ttcgatcgct
     2041 cgtcaatact gtgcgcccga tgaatccgca gagagaacag aagagaacgc tcgcaatcgt
     2101 gttcgctctc cttgcggccg gaggtctcat cgttggctgc ttcggtccga agtggctcgt
     2161 cagcgtgcgc gggcctcgga tggcgatcgg gctgcgcaac ttccaggtct gcgccggcgg
     2221 gcagtgcgag gcgatctcga acttccagat ggtcgacgag ctcgacaagc agatcgcgaa
     2281 gctgaaagaa acctacgcgg atgcaccgct cggcgtacag aggcaggcga tccccgagaa
     2341 tccgtggcat ggcttccccg tcgtcggttg gatcacgctc gcgatggggc tcatctcggc
     2401 gatcgggctc gtctggggtg ccgttctcgc gcttcaacgc aagcgtcccg atctgcccat
     2461 catgccgacg acactcgcgg tgatcggtct cgcgctcggc atcgtcaacg gctgcctcgt
     2521 cctcgcgacg aagcccgaca tcatcgacga catcgcggtc ggctggtcgt tctgggcatt
     2581 cggcgcaggc gcagtgctcg ggctcaccgc ggtctttccg ctcaatcgcc agattcgtcc
     2641 gatcgatgaa gagctcggcg aggcgtccgc gacgatgtcg tggggcgcaa gccgcgacga
     2701 cgaatagcta cggctcgagg acgatctcct cgacgactgg ctcgaacgcc tcggtctcca
     2761 tcgtctcgag ctcggcatct cgtacaacgg atgcgaatgc cgcatgcatg tgctcgatct
     2821 gcatccgtgc gacgtagccc aacgcgaccg cgcattgccc gaggcgctcg cccggcatcc
     2881 gatcctgcag ctgcaacgcg cggaacaact gaaagcgatc gaggacacgc gccatgacca
     2941 agaactcgcc aaatgccgcc cgcttcggtg ccgcgagctt gctcagctcg atgatctccg
     3001 tctgcatctc tctgggaaga gccgactgca aacgaccggt cgcaggaaaa aacccgcttc
     3061 agttcgacgg atgccgcgac tccgcgtcgc gatcgacgtc ggccgccgct gcgtcgagcg
     3121 tcgcaccggc gagcagcgca ccgacacctg ccagcggtga gccggtgccc atgaggatct
     3181 cgcctgccat cgtcgccgcg atcgatccgc cgtggagcgc gccagagcgt gcggtgcgcg
     3241 gccgatagtg acccgcgccg atgaaccagc cgttgccgtc gacgccgacc atcgcgcccg
     3301 gctcgggata gcgccagttg tcctcgaacc gctccgctgc aacacgcgct gcttcgattc
     3361 gcagcgacac gggcgggggc tgcggcgtcg gatcggagaa cgagaaaccc gagagtacga
     3421 gggtgggacg ttgcggcgga tcggccgacg ccgtcgccgt cgcgaggacg aggaggacga
     3481 agctgttgcg caaggcaccc atgacacgat caaaccacga ttttccgacg acggatcggg
     3541 gaatcacgcg atgatcacgt tcgccacgca agtcctcgat acaattcgcg cgtgacgtgg
     3601 acgatccgcc ccgtggaccg caacgccgat cgacgggccg tgtcggcgat cgatacgagc
     3661 ttcgagacga catcgatcta cgaggtcgtc gcaaccgctc gttctctcga gctcgtcgag
     3721 cgaccagcga cggtcacgaa gcggtatccg atggcagatg cgttcgctcc gtggtcgacg
     3781 tgggacaccg gctgggtcgc ccattcgggt gacgaggcgc tcggatttgc cggcgtcgaa
     3841 ttcgaagcgt ggcacgcgcg tctcgtgctc tggcatctct acgtcacgcg ctcgcatcgc
     3901 cgcactggga tcggtcgcgc attgctcgcg gaggtcgaga gctacggacg ttcgctcggc
     3961 gcgcgtcgcg tgtggctcga aacgacgagc gtcaacgtgc cgggcatcgc tgcgtacgcg
     4021 cgtctcggct actcgctcgt cggtctcgac acgtcggtct acgacacgct gccgtattcc
     4081 aacgaggctg caatctatct cgcgaagtca ctctgatcgt cctagctcgc gacgcgctcg
     4141 ggctcgctga cgaacgcctc gacctggagt gtttcgaggt cgagctgttc gccgtttgcg
     4201 gcgaagcgga cgcgcagctt gtctttgagc gcaagcgcgc gaaagaagtg cagcacctgt
     4261 tggtcgatca acatctcgtc gaagccagga ggagtcctca tcgccagatc gacccggcgc
     4321 gcgcgccctg gtcgcaacca aatacaattc agtccgcaat tgccggaatt tgctgaggcc
     4381 gccgtcacat gcgggcgagc gcgtcgttca tcgactcgac gagcaggccg aggtcgacga
     4441 gcgcatcgtc gcgatctttg ccgatcggat cgccgagcag caggatatgg ctgcctcgca
     4501 gcggcgtgac gaccggatgc atcacgccga gcgcttttgt ggtgacctcg tattcggacc
     4561 caggcgcggc ggcatcgccg agatccgcga tatcgacggc caacgtcttc acgagattcg
     4621 gactgatgcg tgcgccgtga ccgcgaatgc cgatcgcacg ctgttcgcgc acgacgagca
     4681 atagcgagga ggtccagtgc gtcgagatgt acttcatcgc gacgtcgaac agccactctg
     4741 catcatcgat ctcgcggaac gacgcgagcg tcgcatcgag ggcgtcgcgt tcgcttgtga
     4801 ctgcgagcgt tgggatcacg acggagagag cccgttgctt cttcttcggc ttcggtagct
     4861 cttccacatc gatggggatg tcgaagtctt ccacaacttg cggcacctct tgcagctcct
     4921 cttctagctc gctcgagtcg agctcgcgcg gtgcgtacgc ggcctcgacg attcgctcga
     4981 ggtagagcgc gggcgcgatg gccaagacga cctcttcgcg cacgattcgc gtcagctttg
     5041 cttggagcgc ggcggatggt tcgcgcacgc agatgatcag cgtcccgttt gcgagccggc
     5101 cgatcggcaa tgcgacgagc tgctttgcga tgtcgtcggg caagagatcg gcgagcgact
     5161 cgtcgcgacc ctcgaggtga cgccgcagtg cggccgcgct gtggtggact tcaccgagca
     5221 gacgcgaggc ttcgtcgaaa tcgagcaggc cacgcgacac gaggagcgag cacagccgca
     5281 tgccgctacc cgcctggtca cccaatgcga gcgcgagtgg ctcccagtcc accaaaccgc
     5341 gttgaagc
//
LOCUS       DATLBZ010000047         3166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2272465, whole genome shotgun sequence.
ACCESSION   DATLBZ010000047 DATLBZ010000000
VERSION     DATLBZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272465"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1121)
                     /gene="holA"
                     /locus_tag="VMZ53_02310"
     CDS             complement(90..1121)
                     /gene="holA"
                     /locus_tag="VMZ53_02310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HUS27303.1"
                     /translation="MHELVADGLDPVYVLHSEHPIMIERAVTAIRDAAVPPATRGFNY
                     DVIEGKPKGNQIVSLAQTLPMMAKSRMVFVRDIGLLPADEAEPVIAYLAKPNPSTVLV
                     AVTNKLDKRLKLFAQLSKKGFLHVLEAPRQVGPWVREEAKTQGVKMDANAINRLIDTV
                     GNDLSRLALSIGQLGLYAGDRAVTSDDVDELVADTRERSVFELTDAIGAADRKRALAA
                     VASLCDQRESAVGVVVMLARHIRQLSLLHHLRSTNVPRPEWASRVGVPPFIVDKLIQQ
                     ARGYSPEALAQATQRLANADRALKGDITLTTQTAEFTGPQLKALGRELAERVILEQIV
                     DGIVGLAGA"
     gene            1166..2503
                     /locus_tag="VMZ53_02315"
     CDS             1166..2503
                     /locus_tag="VMZ53_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUS27304.1"
                     /translation="MADRVVVIADRALLPDGARGPTAILAEDGAIVAVDEPATIAADP
                     RAAGARAIDWSRRAVVPGTVNTHNHSFQSLLRGIGDDLPFLEWRDKALYRYSPRLGAE
                     DMATAALFAFGEMLLHGVTTVCDFYYINAQGNDHASATIEAARRLGIRMVMARCFYDW
                     DGAPASYRETIPQAIANYEALHKRYRDKARHMVTVQPAPHSQHGATPAMIEAGAGCAA
                     DAGEPWHIHLAEESYQVEQSLKRFGKRPLHAVSELAVDMSTMIAVHGCWFDETERALL
                     AERGGSLAYCPGSNMFLGDGVTDLVDLVARGVRVGLGTDGGCSNNRVSVFDEMRAAAL
                     LQKVHRTDGQAITAETCFALGTRTAGDVLRLPIGQIAPGYRCDLVALDLDDPSLWPVQ
                     ALEKNVVYALSSRAITDVVVDGQEVVSARRLRNVQLDEIQARVTELTRDWRRE"
     gene            2439..3026
                     /locus_tag="VMZ53_02320"
     CDS             2439..3026
                     /locus_tag="VMZ53_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27305.1"
                     /translation="MSSSTKSKPASPSSPATGGENKGDKSDSRGKPSKNDSSSGRRDI
                     VEIELEHSRQREIAWTIGGVIGGIILITKLGTVGVWGGYALIAYGLYKAYLLVMSFVN
                     PPGTVIVTDRKVVLPRGLHRGNPLEVTPTEVTAVYFLRRSVPWNKSSPVLVVELGEKA
                     MIFPRDWFATEADQRHIVHALLTQMRPAAPAAAAE"
BASE COUNT          539 a         1075 c         1027 g          525 t
ORIGIN      
        1 gatcgacgtc gacgaaacgt gaaccttggg tgcccacagg cgtgccgccc accccaccgg
       61 actcgccacc gagaagcagc acatgggtgc tacgcacccg ccagcccgac aataccgtcg
      121 acgatctgct cgaggatcac acgctccgcg agctcgcgtc cgagcgcctt gagctgaggc
      181 ccggtgaact cggcggtctg cgtggtgagc gtgatgtcgc ccttgagcgc gcgatcggcg
      241 ttcgcgagac gctgcgtcgc ttgcgcgagc gcctcgggcg agtagccgcg tgcttgctgg
      301 atgagcttgt cgacgatgaa tggcggcacg ccgacgcgac tcgcccactc cggccgcggc
      361 acgttggtcg agcgcaggtg atgcagcagc gagagctggc gaatgtgtcg cgcgagcatc
      421 acgacgacgc cgaccgcgct ctcgcgctga tcgcacagcg aagccactgc ggcgagcgcg
      481 cgcttgcggt ccgctgcgcc gatcgcatcg gtgagctcga agaccgagcg ttcgcgcgta
      541 tcggcgacga gctcgtcgac atcgtcggac gtcactgcac gatcgccggc gtacaggccg
      601 agctggccga tcgagagcgc gaggcgcgag aggtcgttgc cgacggtatc gatcagacga
      661 ttgatcgcgt tggcgtccat cttcacgccc tgcgtcttgg cttcttcgcg cacccagggc
      721 ccgacttggc gcggcgcctc gagcacgtgg aggaagccct tcttcgagag ctgcgcgaac
      781 agcttgagac gcttgtcgag cttgttcgtc actgcgacga gcaccgtcga cggattgggc
      841 ttcgcgagat acgcgatgac gggctccgct tcgtccgcgg gcagaagacc gatgtcgcgc
      901 acgaacacca tgcggctctt cgccatcatc ggcagcgtct gcgcgagcga gacgatctgg
      961 ttgcccttgg gcttgccctc gatgacgtcg taattgaagc cgcgcgtcgc cggcggcacg
     1021 gccgcatcgc ggatcgcggt gaccgcacgc tcgatcatga tcggatgctc tgaatggagg
     1081 acgtagaccg ggtccaatcc gtctgccacc agctcgtgca cgaggcgcaa gctaccacgg
     1141 gggtctgagt gtaggctccg caacaatggc agaccgagtt gtcgtcatcg ccgatcgcgc
     1201 gctcctgccc gacggtgcac ggggcccgac ggcgattctc gcggaagatg gcgccatcgt
     1261 tgcggtcgac gagccagcga cgatcgcagc cgatccccgg gcagccggcg cgcgtgcgat
     1321 cgactggtcc cgtcgggcgg tcgtgcccgg cacggtgaac acgcacaacc acagcttcca
     1381 gtcgctcctc cgcggaatcg gcgacgacct gccgtttctc gagtggcgcg acaaagcgct
     1441 ctatcgctac tcgccgcggc tcggcgccga ggacatggcg accgccgccc tgttcgcgtt
     1501 cggcgagatg ctgctccacg gcgtcacgac cgtttgcgac ttctactaca tcaacgcgca
     1561 gggcaacgac cacgcctccg cgacgatcga agcggcccgc cgccttggta ttcgcatggt
     1621 gatggcgcgc tgcttctacg actgggacgg cgcgccggcg tcgtatcgcg agaccatccc
     1681 gcaagcgatc gcgaactacg aagcgctgca caaacgctat cgcgacaagg cgcgtcacat
     1741 ggtcacggtg cagcccgcgc cacacagcca gcatggcgca acaccggcga tgatcgaagc
     1801 cggtgcaggc tgcgccgccg atgcaggcga gccgtggcac atccacctcg ccgaggagtc
     1861 ataccaagtc gagcaatcgc tgaagcggtt cggcaagcgc ccgctccacg cggtctccga
     1921 gctcgccgtc gacatgtcga ccatgatcgc cgtgcacggt tgctggttcg acgagaccga
     1981 gcgcgcattg ctcgccgagc gcggtggcag cctcgcgtac tgcccggggt cgaacatgtt
     2041 cctcggtgat ggcgttacgg acctcgtcga tctcgtcgcg cgcggcgttc gcgtgggcct
     2101 tggcaccgac ggcggctgct cgaacaaccg cgtctccgtc ttcgacgaaa tgcgcgcagc
     2161 cgcgttgctc cagaaggtgc accgcaccga cggtcaggcg atcaccgcgg agacgtgctt
     2221 cgcgctcggc acgcgcaccg caggcgacgt cctgcgcctc ccgatcgggc aaatcgcgcc
     2281 aggctaccgc tgcgacctcg tcgcgctcga tctggatgat ccgtcgctct ggccggttca
     2341 ggccctcgaa aagaacgtcg tctacgcgct ctcgtctcgc gcgatcaccg acgtcgtggt
     2401 agatggacag gaagtcgttt ccgcccgccg tctacgcaat gtccagctcg acgaaatcca
     2461 agcccgcgtc accgagctca cccgcgactg gcggcgagaa taaaggcgac aagtccgata
     2521 gccgcggcaa gccgtcgaag aacgactcct cgtcggggcg ccgcgacatc gtcgagatcg
     2581 agctcgagca ttcgcgccag cgtgaaatcg cgtggaccat cggcggagtg atcggcggca
     2641 tcatcctgat cacaaagctc ggcacggtcg gcgtgtgggg cggctacgcg ctgatcgcgt
     2701 acggcctcta caaggcgtac ctgctcgtga tgtcgttcgt gaatccgccg ggcacggtga
     2761 tcgtgaccga tcgcaaggtc gtgcttccgc gcggccttca ccgcggcaac ccgctcgagg
     2821 tcacgccgac tgaagtcacc gccgtctact tccttcgccg ctctgtgccg tggaacaagt
     2881 catccccggt tctcgtcgtc gagctcggtg agaaggcgat gatctttccg cgcgattggt
     2941 tcgcgaccga agccgaccaa cgccacatcg tgcacgcgct actcacgcag atgcgtcctg
     3001 ccgcacccgc cgccgccgcc gaatgaaacg cgcactgctc gctctgctgt tgctcacggg
     3061 cgcggcgaac gcgggcgatg tcgagcttcc gaagtcgaca gcgcatctga tcatttccga
     3121 ggactggaag gccgtgcgag cacgaggagt cgttgcggca tacaag
//
LOCUS       DATLBZ010000048         8608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8976420, whole genome shotgun sequence.
ACCESSION   DATLBZ010000048 DATLBZ010000000
VERSION     DATLBZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8976420"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1030)
                     /gene="hemC"
                     /locus_tag="VMZ53_02325"
     CDS             complement(95..1030)
                     /gene="hemC"
                     /locus_tag="VMZ53_02325"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HUS27306.1"
                     /translation="MSTPERLVIATRGSELALWQANHVKSQLERVAPGITVELNVIKT
                     SGDKILDVPLAKVGGKGLFVTEIEQALVDKQADLAVHSMKDVPAELAPGLTICAVSTR
                     EVPWDALCTRDGKTLDQLPQGAVVGTSSMRRQAQLLAVRPDLQIKMLRGNVPTRLRKL
                     DDGEFDAIVLAAAGLHRLGLANRISHELPLEISIPAVAQGVLAIEIRDGDGSVFQIVR
                     KAMHDEPTYKKMLAERAFLARMGGSCQTPLAAHAIELPDGNLRLIAMCGMPDGTRILK
                     AEREGAIAQAAQLGTELGDELLKQGAGEILAATKL"
     gene            complement(1027..2382)
                     /gene="hemA"
                     /locus_tag="VMZ53_02330"
     CDS             complement(1027..2382)
                     /gene="hemA"
                     /locus_tag="VMZ53_02330"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HUS27307.1"
                     /translation="MSELFVVGISWRTAPVAVREKLAFREEELTSTLQKITAQLPVAE
                     ALLISTCNRVEVYGVARGPAEPTAAVRQFLTEQRQVKPKDLGDAVYSHAGPAAVRHVF
                     RVASALDSLVVGEAQILGQLKAAYSVAGKAGTSGPVLSRALERAFGVAKRVRTETTIA
                     RGAANVSSVAVELAGRVFGNLTGKSVLVVGAGKMSSLAAKHLYANGAQRIVVTNRSPE
                     KADALAEEIEGVAKPWAELEQLLAEADVVISSTGAREPVLTKKLFKAVTKARRWRQMM
                     VIDIAVPRDAEPAIGELDGVYLFDIDDLDKVVQANIAERQKAAEHASKIVEHEAGQFE
                     HWLRTQNVVPTIRALREKFAQVADAEVQKALDQLARKDLTRDQQRELLQRSVQLVVNK
                     LLHQPTTVLRGADPAEAQELAGAVCELFGLDPNESPEDVADPDAAEQAATTSKQEKAQ
                     A"
     gene            complement(2379..3176)
                     /gene="ccsA"
                     /locus_tag="VMZ53_02335"
     CDS             complement(2379..3176)
                     /gene="ccsA"
                     /locus_tag="VMZ53_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HUS27308.1"
                     /translation="MLTVPPSPAFWVAVAMYAAAASALLFVLAGNSKARPLALGLVVC
                     AFVAHGFDIGWRGTQHVHPAQSVREALGFLAFIVTGGYLLASLRYRLTLGGVVVMPVA
                     LVLLLLARLTPAGQAQDLGSLGRIHITLATVGVGVFALAGALSAIYLAEDRALKRKKF
                     DGLAFKDRGAPLEALDRLAHRLIWVGFPIFTISLVLGAIWVSRLGESLARVEYALATV
                     TWLAYATLLVARGIYGWRGRRAAILTLEGFAAALSVLVIYLIRRAVG"
     gene            complement(3210..3512)
                     /locus_tag="VMZ53_02340"
     CDS             complement(3210..3512)
                     /locus_tag="VMZ53_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27309.1"
                     /translation="MSRLFISVDRLDAWTSEGRAALEGDHMTLIELGRRFSMTPAVCF
                     VKTMGGGEDDPYDLVGRVKSKPQLDEMGAEQLANSVIYQDMAYDVIDGFIGEPLPP"
     gene            3600..3935
                     /gene="trxA"
                     /locus_tag="VMZ53_02345"
     CDS             3600..3935
                     /gene="trxA"
                     /locus_tag="VMZ53_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HUS27310.1"
                     /translation="MASQNVKELTDANFESEVLKSDIPTLVDFWAVWCGPCKQIAPTV
                     DALADEYKGKLKVAKMDVDHHQIVPQQYRVTSIPTLLIFKGGRVVSQLVGAMPRSKLE
                     AEIQKHIAG"
     gene            3935..4279
                     /locus_tag="VMZ53_02350"
     CDS             3935..4279
                     /locus_tag="VMZ53_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27311.1"
                     /translation="MRFLALALAVAALLPGCGKSPSAELGECQAVAEKSRELRQRDLE
                     STDPGSPALRQLDKIARATAASCAEDHWPRELRECVMKADTALALAQCHAKLMPPEMQ
                     RRVSDRVLEATK"
     gene            complement(4291..5739)
                     /locus_tag="VMZ53_02355"
     CDS             complement(4291..5739)
                     /locus_tag="VMZ53_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HUS27312.1"
                     /translation="MATSRSTTQASKFLSLILRHEPEKYGIALDEAGWTDIDKLLDAL
                     HAHGAELTRAELDEIVRSSDKQRFAISSDGTKIRANQGHSVQVELGLPTETPPAVLYH
                     GTIEAVLAPIRKDGLVKGQRHHVHLSADMETAKKVGGRRGKPIILAIKAAEMVAKGHT
                     FQRSANGVWLVDHVPPEFIELDDTVAHHDLVPHGGGTVSRQQKIQIAKDSLAACEAGF
                     YENAKGVRVELAAAIEEAKAGTGLYERGVDRLEPPRKWSGPDSLGDAGKTRAQTKISV
                     TGETTIDAMVRLAKSGGHLGCLNFASAKRPGGGFLGGAQAQEESLARASALYPCLQTH
                     PEYYQRNKDFHSPLYLDIILYSPLVPFFRDDNGGWFDAPLLASVITAAAPNASALREQ
                     KKFDAEDVADVLRTRSDFVLAIAAHHKIDRLVLGAWGAGVFGNDPALVAKIFADLLRG
                     PYAGAFVEVVFAVLGTRETSVNHKAFADVFGG"
     gene            complement(5729..6868)
                     /gene="rodA"
                     /locus_tag="VMZ53_02360"
     CDS             complement(5729..6868)
                     /gene="rodA"
                     /locus_tag="VMZ53_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HUS27313.1"
                     /translation="MRGGLLLPRATIGVTRWRRFRAAVDWPLILTVIVLCAIGLLNLY
                     SATRSPRAVSKFDAQVQWMIVGAISFVIVTIVDYRTILRAAPILFALAIAALISARLF
                     GSMSDEHGVTFRWIYFAGVRFQPSEAAKIAVILMLAQACSNGEASTYTPRSIVMTFGA
                     FLVPIVLIAIQPDLGTAVLLTLIVLTVGYLAMPTVWPMVYMTAAGLLAIPILWEKMHE
                     YQKNRLLSFVDCGSVPNKMCWHTQQSIYAVGSGKIWGKGFLGGTQNQFNFVPEHWTDF
                     PYSVWAEEWGFAGSVFLLAVFGFLLLWTINVALSARDRMGSIICLGVAAMTFWHVVVN
                     VAMVLGIAPVVGVTLPFISYGGSSLVTFFISMGLVANVSLRKHGY"
     gene            complement(6865..>8608)
                     /gene="mrdA"
                     /locus_tag="VMZ53_02365"
     CDS             complement(6865..>8608)
                     /gene="mrdA"
                     /locus_tag="VMZ53_02365"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HUS27314.1"
                     /translation="RPAFNIYATPKQLTPDAKAELVRMLGLSDDEIGKLDERIAVGVK
                     RDAKQPILVLEDQGRDRAALVEQAKARLPGVEVHHEPYRYYPQGDLAAHLVGYMTQMT
                     AEEADRLTAQGYDASDLVGRYGLESAWENYLRGKRGVEHYAVDARGQRIDNKTVDSLI
                     HGDRVTEPVPGANLILTIDSDLQKLAEKAVSHVAASAVVVVEVKTGKVKAIVSKPSFD
                     PNVMTGHLTHAEYTLLMQDPRKPFIDKALRATYPPGSIFKFVTTIAALEDGQAAEDET
                     MFCPGMFSLSGTHFRCHGYHEKVDLLRAIQKSCDVYFWNLATRIGLDRIAEVAHEFGL
                     GTPTNLGINGDSGGRIPTKQWYEKNGRFMVGYAINAAIGQGDVEVTPIQMAMAYAALA
                     NGGNVFVPQVVERVESFEGRTIVSYDPKVAHVAKVPPDAIETWKRGMWLVNNEKGGSA
                     FDHGEPTGIVTVMGKTGTAEVKKHHKDADDKERDKWNPNASHAWFAGWAPVEDPELAI
                     IVLVEHGGGGGANAWPIAQRILDGYFGKLKPAAQAKAAQAAAPPIPPATNASMKVPPQ
                     SKKPPTNVKKKDLP"
BASE COUNT         1408 a         2830 c         2872 g         1498 t
ORIGIN      
        1 cgagcacgag cacgagcacg agcacgagca cgagcacgag cacgagcacg agcacgcaag
       61 tgctcgcacg atcacgacgc gggcgctacg agcgctacag cttcgtggcg gcgaggattt
      121 cgccggcgcc ctgctttagc agctcgtcgc ccagctccgt gccgagctgc gcggcctgtg
      181 cgatcgcgcc ttcacgctcg gccttgagga tgcgcgtgcc gtcgggcatg ccgcacatcg
      241 cgatcaggcg caggttgccg tccggcagct cgatcgcgtg cgcggcaagc ggcgtttggc
      301 agctaccgcc cattcgcgcg aggaacgcgc gctccgcgag catcttcttg taggtcggct
      361 cgtcgtgcat cgccttgcgc acgatctgga agacggagcc gtcgccatcg cggatctcga
      421 ttgcgagcac gccttgtgcg accgcgggga tgctgatttc gagcggtagc tcgtggctga
      481 tgcggttggc gaggccgagg cggtgaaggc cggcggccgc gagcacgatc gcgtcgaact
      541 cgccgtcgtc gagcttgcgg aggcgcgtgg ggacgttgcc gcgcagcatt ttgatctgga
      601 ggtcggggcg cacggcgagc agctgcgctt ggcggcgcat gctggatgtt ccgacgacag
      661 cgccttgagg gagctgatcg agcgtcttgc cgtcgcgcgt gcagagcgcg tcccatggga
      721 cttcgcgcgt cgagaccgcg cagatcgtga ggcctggtgc gagctcggcc ggcacgtcct
      781 tcatcgagtg cacggctagg tcggcttgtt tgtcgacgag cgcttgctcg atctctgtga
      841 cgaagaggcc tttgccgccg actttcgcga gcgggacgtc gaggatcttg tcgccgctcg
      901 tcttgatgac gttgagctcg acggtgatgc ccggcgcgac gcgttcgagc tgcgacttga
      961 cgtggttcgc ttgccacaag gcgagctcgc tgccgcgagt agcgatgacg agacgttcag
     1021 gagtgctcac gcttgtgcct tctcttgctt gctggtggtg gcagcttgct ccgcggcgtc
     1081 ggggtcggca acgtcttcgg ggctctcgtt cgggtcgaga ccgaacagct cgcacaccgc
     1141 gcccgcgagc tcctgcgcct cggccgggtc ggcgccgcgc agcactgtcg tcggttggtg
     1201 cagcagcttg ttgacgacga gctgcacgct gcgctggagg agctcgcgct gttggtcgcg
     1261 cgtgagatcc ttgcgcgcga gctggtcgag cgccttttgc acctcggcgt cggcgacttg
     1321 cgcgaacttc tcgcgaagcg cacgaatcgt cggcaccacg ttttgggtgc ggagccagtg
     1381 ctcgaattgg ccggcttcgt gctcgacgat cttggacgcg tgctcggcgg ccttttggcg
     1441 ctcggcgatg ttcgcttgca cgaccttgtc gagatcgtcg atgtcgaaga ggtagacacc
     1501 gtcgagctcg ccgattgcgg gctcggcgtc gcgcggcacc gcgatgtcga tcaccatcat
     1561 ctggcgccag cgccgcgctt tcgtcacggc cttgaacagc ttcttcgtca gcacgggctc
     1621 gcgtgcgccc gtcgacgaga tcacgacgtc ggcttcggcg agcagctgct cgagctcggc
     1681 ccacggcttt gcgacgccct cgatttcttc ggcgagcgca tcggcttttt cgggcgagcg
     1741 attcgtcacg acgatgcgtt gtgcaccgtt ggcgtagaga tgcttcgcgg cgagcgagct
     1801 catcttcccc gcgccgacga cgagcacgct cttgcccgtg aggtttccga acacgcgccc
     1861 cgcgagctcg accgcgacgg aggagacgtt tgcggcgcca cgggcgatcg tcgtctccgt
     1921 gcgcacgcgc ttcgcgacac cgaacgctcg ctcgagtgcg cgcgagagta cgggacctga
     1981 cgtgccggcc ttgcctgcga cgctgtaggc cgctttcagc tggccgagga tctgcgcttc
     2041 gcctaccacg agggagtcga gcgccgacgc gacgcggaat acgtggcgca cagcggcggg
     2101 gccggcgtgc gaatagacgg cgtcgccgag atccttcggc ttcacctggc gctgctcggt
     2161 gaggaactgg cgaactgcgg ccgtgggctc ggccgggcct ctcgcgacgc cgtagacctc
     2221 gacgcggttg cacgtcgaga tgagcagcgc ttcggcgacc ggcagctgcg ccgtgatctt
     2281 ctggagcgtg ctggtcagct cttcttcgcg aaacgcgagc ttctcgcgga ccgcgacggg
     2341 tgccgttcgc cagctgatgc cgacgacgaa cagctcgctc atcccaccgc ccggcgaatg
     2401 agatagatga cgagcaccga gagcgccgcg gcgaagcctt cgagcgtgag gatcgcagcg
     2461 cgtcgtccgc gccagccgta gatgccacgt gcgacgagca gcgtcgcgta tgccagccac
     2521 gtgacggtcg cgagcgcgta ctcgacacgc gcgagactct cgccgagacg cgacacccag
     2581 atcgcgccga gcacgagcga gatcgtgaag atcgggaagc cgacccaaat caggcgatgc
     2641 gccaagcgat cgagtgcttc gagcggtgcg ccacgatctt tgaacgcgag gccgtcgaac
     2701 ttcttgcgct tgagcgcgcg atcctcggcg agatagatcg cggacagcgc gcccgcgagc
     2761 gcgaacacgc cgacgcccac cgtcgcgagc gtgatgtgaa tccgcccgag cgatcctaga
     2821 tcctgcgcct gccccgccgg cgtgagccgc gcgagcaaga gcaacacgag cgcgacgggc
     2881 atcacgacga caccgccgag cgtgaggcgg tagcgcaagc tcgcgagcaa gtagccgcct
     2941 gtcacgatga acgcgagaaa cccgagggcc tcgcgcaccg actgcgcagg atgaacatgc
     3001 tgcgtgccgc gccagccgat gtcgaagccg tgcgcgacga acgcgcagac gacgagcccg
     3061 agcgcgagcg gccgcgcctt cgagtttccg gcgagcacga acagcagcgc ggacgctgct
     3121 gcggcgtaca tcgcgaccgc cacccagaac gcgggtgacg gagggaccgt gagcaaccgg
     3181 tcgctaaggt cgctgcccgg catggcgcct cacggcggga gcggctcgcc gataaacccg
     3241 tcgatcacgt cgtacgccat gtcttggtag atgacggagt tcgcgagctg ctctgcgccc
     3301 atctcgtcga gctgcggctt gctcttcacg cgaccgacga ggtcatacgg atcgtcctcg
     3361 ccgccaccca tcgtcttcac gaagcacacg gcgggagtca tcgaaaaccg ccgcccgagc
     3421 tcgatcagcg tcatgtggtc gccttcgagc gcggcccgac cttcggaggt ccaggcatcg
     3481 agacgatcca ccgagatgaa gaggcgcgac atccttgccg taattgtacc accgcccgtt
     3541 agacccggtt gcgctcttcg cggcggggag ttacataagc ccaaccaccg gaggtccacg
     3601 tggctagcca gaacgtcaaa gagctcaccg acgcgaactt cgagagcgaa gttctgaagt
     3661 ccgacatccc caccctcgtc gatttctggg ccgtctggtg tggcccctgt aagcagatcg
     3721 ccccgaccgt cgacgcgctc gccgacgagt acaagggcaa gctgaaggtc gccaagatgg
     3781 acgtcgacca tcaccagatc gttccgcagc agtaccgcgt gacgtcgatc ccgacgctgc
     3841 tcatcttcaa gggcggccgc gtggtcagcc agctcgtcgg cgcgatgccg cgcagcaagc
     3901 tcgaagccga gatccaaaag cacatcgccg gttagtgcgt ttcctcgccc tcgccctcgc
     3961 tgtcgctgca ctgcttcctg gctgcgggaa aagtccttcg gctgagctcg gcgagtgcca
     4021 ggcggtcgct gaaaagtcgc gcgagctcag gcagcgggac ctggaaagca ccgatcccgg
     4081 ctcgcctgcg ctacgccagc tcgacaagat cgctcgcgcg accgccgcat cgtgcgccga
     4141 agatcattgg ccgcgcgagc tccgggagtg cgtgatgaag gcagacaccg cgctcgcgct
     4201 cgcgcagtgc cacgcgaagc tcatgccgcc cgagatgcag cgtcgggtat ccgaccgcgt
     4261 cctcgaggcg acgaagtagc cagccgtccg ctagccgccg aagacgtcgg cgaacgcctt
     4321 gtggttcacg cttgtctcgc gcgtgccgag caccgcgaac accacttcga cgaatgcgcc
     4381 ggcatacggc ccgcgtagca ggtccgcgaa gatcttcgcg acgagcgcgg gatcgttgcc
     4441 gaacacaccc gcgccccatg cgccgaggac gaggcgatcg atcttgtgat gcgctgcgat
     4501 cgcgagcacg aagtcgctgc gcgtgcgcaa gacgtccgcg acatcctcgg cgtcgaactt
     4561 cttctgctcg cgcagcgcac tcgcgttcgg cgccgccgcg gtgatcaccg aggcgagcag
     4621 cggcgcgtcg aaccaaccgc cgttgtcgtc gcgaaagaac ggcaccagcg gtgagtagag
     4681 gatgatgtcg aggtagagcg gcgagtgaaa gtctttgttg cgctggtagt actcggggtg
     4741 cgtctgcagg cacggataca gcgccgacgc gcgagcgagc gattcttcct gcgcttgcgc
     4801 accgccgagg aagccgccgc ccgggcgctt cgccgacgcg aagttgagac agccgagatg
     4861 cccgcccgac ttcgcgaggc gaaccatcgc gtcgatcgtc gtctcgccgg tcacgctgat
     4921 cttcgtctgc gcgcgcgtct ttcccgcgtc cccgagtgaa tcggggccgc tccacttccg
     4981 gggtggctcg agacgatcca cgccgcgctc gtagagcccg gtgcctgcct tcgcctcttc
     5041 gatcgcagcg gcgagctcga cgcgcacgcc cttcgcgttt tcgtagaagc cggcctcgca
     5101 cgcggcgagc gagtctttcg cgatctggat cttctgctga cggctcaccg tgccgccgcc
     5161 gtgcggaacg aggtcgtgat gcgcgaccgt gtcgtccagc tcgatgaact cgggcggtac
     5221 gtgatcgacg agccacacgc cgttcgccga gcgctggaac gtgtggccct tcgcgaccat
     5281 ttcggcggct ttgatcgcga gaatgatcgg cttgccgcgc cggccgccga ccttcttcgc
     5341 ggtctccata tccgcggaga gatggacgtg gtggcgctgg cctttcacga ggccgtcttt
     5401 gcgaatcggc gcgagcaccg cttcgatcgt gccgtgatag aggacggctg ggggcgtctc
     5461 ggtcggaagg ccgagctcga cttgcaccga gtggccttgg ttcgcgcgga tcttcgtgcc
     5521 gtcggacgag atcgcgaacc gctgcttgtc gctcgaccgc acgatctcgt cgagctcggc
     5581 gcgcgtcagc tctgcaccgt gggcgtggag cgcgtcgagc agcttgtcga tgtccgtcca
     5641 gcccgcctcg tcgagagcga tgccgtactt ctcgggctcg tgccggagga tcagcgacag
     5701 gaacttgctc gcctgcgtgg tggagcgact agtagccatg cttgcgcagc gacacgtttg
     5761 cgacgagtcc catgctgatg aagaacgtca cgagcgagct gccaccatag gagatgaacg
     5821 gcagcgtaac gccgacgacg ggcgcgatgc cgagcaccat cgcgacgttg acgacaacgt
     5881 gccagaacgt catcgcggcg acgccgaggc agatgatcga gcccatgcga tcacgcgccg
     5941 agagcgcgac gttgatcgtc cataggagca ggaagccgaa cacggcgagc aagaacaccg
     6001 agcccgcgaa gccccactcc tctgcccaca ccgagtatgg aaagtcggtc cagtgctcgg
     6061 ggacgaagtt gaattggttc tgcgtgccgc cgaggaaacc cttgccccaa atctttccag
     6121 agcccaccgc gtagatcgat tgctgcgtgt gccagcacat cttgttcggc acgctgccgc
     6181 agtcgacgaa cgagagcagc cggttcttct ggtactcgtg catcttctcc cagaggatcg
     6241 ggatggcgag tagaccggcc gcggtcatgt agaccatcgg ccacaccgtc ggcatcgcga
     6301 ggtagcccac cgtgagcacg atcagcgtga gcagcaccgc ggtgccgagg tcgggctgga
     6361 tcgcgatcag gacaatcggg acgaggaacg cgccgaacgt catgacgatc gagcgcggcg
     6421 tgtacgtcga ggcctcgccg ttcgagcacg cctgcgcgag catcaggatc accgcgatct
     6481 tcgcggcctc cgacggctgg aaccgaacgc ctgcgaagta gatccagcgg aacgtcacac
     6541 cgtgctcgtc actcatcgag ccgaacaacc gcgcggatat cagcgctgcg atcgcgagcg
     6601 cgaacaggat cggagcggcg cgcaggatcg ttcggtagtc gacgatcgtg acgatcacga
     6661 acgagatcgc tccgacgatc atccactgga cctgcgcatc gaacttcgag actgcacgcg
     6721 ggctgcgcgt cgccgagtag agattgagca ggccgatcgc gcacagcacg atgaccgtga
     6781 ggatcagcgg ccaatcgacc gccgcgcgga atcgccgcca gcgcgtgacg ccgatcgtgg
     6841 cgcgaggaag gagaaggccg cctctcatgg caggtccttc ttcttcacgt tcgtcggcgg
     6901 cttcttgctc tgcggcggca ccttcatcga cgcgttcgtc gcaggcggaa tcggcggcgc
     6961 cgcagcctgt gcggccttcg cctgcgccgc gggcttgagc ttgccgaagt agccgtcgag
     7021 gatacgctgc gcgatcggcc atgcattcgc accgccgccg ccgccgtgct cgacgagcac
     7081 gatgatcgcg agctcgggat cctcgacagg cgcccagccc gcgaaccacg cgtgcgatgc
     7141 gttcgggttc cacttgtcgc gctctttgtc gtcggcgtcc ttgtggtgct tcttgacctc
     7201 ggcggtgccg gtcttgccca tcaccgtcac gatgccggtc ggctcgccgt gatcgaacgc
     7261 gctgccgccc ttctcgttgt tgacgagcca catgccgcgc ttccaggtct cgatcgcgtc
     7321 cggcgggacc ttcgcgacgt gcgcgacctt cgggtcgtag ctgacgatgg tgcggccctc
     7381 gaatgattcg acgcgctcga cgacctgcgg aacgaacacg ttgccgccgt ttgcgagcgc
     7441 cgcatacgcc atcgccatct ggatcggcgt gacttcgaca tcgccctgcc cgatcgcagc
     7501 gttgatcgcg tagccgacca tgaagcgacc gttcttctcg taccactgct tcgtcggaat
     7561 gcgaccgccg gagtcgccgt tgatgccgag gttcgtcggc gtcccgagtc cgaactcgtg
     7621 cgcgacctcg gcaatgcgat cgagaccgat gcgcgtcgcg aggttccaga agtagacgtc
     7681 gcacgacttt tggatcgcgc gcagcaggtc aaccttctcg tgatagccgt ggcagcggaa
     7741 gtgcgtgccc gacaggctga acatgccggg gcagaacatc gtctcgtcct cggcggcctg
     7801 gccgtcttcg agcgcggcga tcgtggtgac gaacttgaag atcgagcccg gcggatacgt
     7861 cgcgcgcagc gccttgtcga tgaacggctt gcgcgggtcc tgcatcagca gcgtgtactc
     7921 ggcatgcgtc aggtggcccg tcatcacgtt cggatcgaac gacggcttgc tgacgatcgc
     7981 tttgaccttg cccgtcttca cctcgacgac gacgaccgct gacgctgcga cgtgcgagac
     8041 cgccttctcg gcgagcttct gcagatcgct gtcgatcgtc aggatcaagt tcgcgcccgg
     8101 gacgggctcc gtcacgcgat caccatggat cagcgagtcg acggtcttgt tgtcgatgcg
     8161 ttggccacgt gcgtcgacgg cgtagtgctc gacaccgcgc ttcccgcgca ggtagttctc
     8221 ccacgcgctc tcgaggccat agcgtccgac gagatcgctc gcgtcgtagc cctgggcggt
     8281 gaggcgatcg gcctcctccg cggtcatctg cgtcatgtag ccgacgaggt gcgcggcgag
     8341 atcgccctgc ggatagtagc gatacggctc gtggtgaacc tcgacgccgg gcagtcgcgc
     8401 cttcgcttgc tcgacgagtg ctgcgcgatc gcggccctga tcttcgagca cgaggatcgg
     8461 ctgctttgcg tcgcgcttga cgcctaccgc gatgcgttcg tcgagcttgc cgatctcgtc
     8521 gtccgagagg ccgagcatgc gaacgagctc tgccttagcg tcgggcgtga gctgcttggg
     8581 cgtcgcgtag atgttgaacg ctggccgg
//
LOCUS       DATLBZ010000049        29411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4510477, whole genome shotgun sequence.
ACCESSION   DATLBZ010000049 DATLBZ010000000
VERSION     DATLBZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 29411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29411
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4510477"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..965
                     /locus_tag="VMZ53_02370"
     CDS             111..965
                     /locus_tag="VMZ53_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27315.1"
                     /translation="MRRAVASIFVIVAGCGRLSFDARGDTSGSATELHDAVLIASLST
                     NVDDDDPEISFDGLEIYFSRALVTNEDDIYVSMRASVLDEWPAATKVPGINTGAIDKG
                     PGLTSDALTLYFGSDRGGAPQKMYMTTRASRSDPWGAPVLVPELSLARNVGSPCVRGD
                     RIVFRQAGGTGTHDLLHAELVGTTWNTPQLITELNTPGEDSGPFLFGDTLYFDSNRSG
                     GVGSEDIYTAHWDAASGTFEPAVLDTTLSSTAQDTDVSLTADGNTIVFSRNIAGSGTG
                     HDIYTATR"
     gene            complement(968..1159)
                     /locus_tag="VMZ53_02375"
     CDS             complement(968..1159)
                     /locus_tag="VMZ53_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27316.1"
                     /translation="MQDLKQDDLEKVSGGTEAGDVCRDSYAATGGLLGGILTSESFGW
                     GAVPGAILGGMIGRRVCPK"
     gene            complement(1319..1813)
                     /locus_tag="VMZ53_02380"
     CDS             complement(1319..1813)
                     /locus_tag="VMZ53_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27317.1"
                     /translation="MTKYLASILFSIALLPACVADDTEDNAQADVATGKADSASTFTP
                     SGIYVGAGDVSRLELSSGAFRAVIADEISGTATHTGSFKLVHQSGHDYIDLLEGGVHH
                     RLEYSPDLTIPETLMWVRWSSSQPWAELWRFGDDCATAGCDAGETCTSCFGDNVCMPA
                     GTSC"
     gene            complement(1874..2752)
                     /locus_tag="VMZ53_02385"
     CDS             complement(1874..2752)
                     /locus_tag="VMZ53_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS27318.1"
                     /translation="MAETALRTDFLNAVSPAERSRWDTPQLEGELAQIVDEARVCYPE
                     VTVDSSTFMRYLAERAATAPAGWLAELPAGDLYLACACANSNVAAIAVVERRHFPRVE
                     SIVRGKLDATLADEAMQRVREHLFVGDRPHISDYAGRGELGKWLTITAVRAGLRVLRD
                     ARRETPLDEPDLDALVGSASDLELQHLRTRYHADFKAAFAEAFADLEPRERRLLRHNV
                     VDRLGVEAIAELHGAHKSTASRWLKSARDTLIAKTKKRLRTRLAISPTECESILRLLA
                     DHVDVTLEGVLRETTG"
     gene            2785..4659
                     /locus_tag="VMZ53_02390"
     CDS             2785..4659
                     /locus_tag="VMZ53_02390"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HUS27319.1"
                     /translation="MACIGTETLLDLASDKSPARLSRFEHHLDACDECRRLIAELAGL
                     SWMRVACAHDETATTEPQRGTLEVGRVIDRYILIDVIGSGGMGIVYAAYDPRLDRKIA
                     LKVLRPDRDGDERRARLMREAQALAQLRHPNVVAIHDAGETDGQVFVAMELVEGTTLR
                     EWLRAERRDWRGIIAMFLQALHGLAAAHAAGIVHRDFKPDNVLIGDGRAQVADFGLAI
                     TATTSGELAGTPAYMSPEQRRGDVLTSASDQYSFCVALKDALSGTRPPARITAALERG
                     MAPAPADRHVSMEALARALRWRSPIRIPAIITGAACIGLAGFAALSSTRSEAGPTCSV
                     ESAEAKLASVWDREQVKQLVLATNSANAPAILASIEQKVDAYGNAWIAERTDACAATY
                     VRGEQSPERLDQRNACLDTRLQELATRITALRDISAANLATGPAIFDDLVSPSACSSR
                     ASLAHESAPPSMGAVSTFDDGSLASRFGAGWAVSTDVIMGGHSTGKLSVVDGGANGTP
                     NALEITGTVSEGGAISWAGAMFSPGASPMMPVDLSAHHFVRFAARGDGGSYNVLLFSQ
                     TKGDLPAFRPFVAGTTWTQYRFPLAEFDGVNPADIKGLLFASSRQGPFALQIDEIEFE
                     "
     gene            4670..5383
                     /locus_tag="VMZ53_02395"
     CDS             4670..5383
                     /locus_tag="VMZ53_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27320.1"
                     /translation="MPQRIFALAFSSVLLVVTVAACKKADKPAPTADKQVTAAPAGGS
                     AAAAAPTPAEGAGDSDSSSPDYTKPALTEAKIQGYIKSMKDGHNPFDVIGAAAGMMNG
                     KTSIANAQAIVDEQEAVAKKYGFASSEDYMDTAGRIMLGEVTLGAAGSMEQVRQMMVQ
                     SIADYEKQLADPALPAETKASLSEALADSKKSLAEMDASAKSGDQLNAADLELIKKFK
                     TEIEAAEKANAEIQNAKHK"
     gene            5394..6359
                     /locus_tag="VMZ53_02400"
     CDS             5394..6359
                     /locus_tag="VMZ53_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HUS27321.1"
                     /translation="MAGFLFRFRMVPIISSMLAMRRYDARELVREAGLPDEALQGEII
                     APFVRVQKFLELAAAKLEAPLFGLDLAERIPTGAFGLTEFLMRSAPSVERSLQVLIEF
                     GSLVNPMLDFRFTTTEHEGVFRFAVPSQRDALGMHLNEYTLMLVARQFSLVHGERLTF
                     ERAWFAHGRRTHAEEVAARLGCSVAFQAADCGFALSRAWLSRSSPISDGALFEFLLNQ
                     ARTQLANLGSRDIVSQVASVIETRLSSGALDAGDIASAMATTLRSLQRHLADAGTSYR
                     EVLQHVRLRRRAELAHSGLTPAEIAQRLGFSNVTSMKRSLDESQD"
     gene            6450..7400
                     /locus_tag="VMZ53_02405"
     CDS             6450..7400
                     /locus_tag="VMZ53_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27322.1"
                     /translation="MRIWPLVLPIVLAATQAWALEPAKHRSLAEKSCADVGLPDAYCR
                     RMGKEVYQTDYREWTDLHAHAQRELGEDRCTAADAAQSRVDRLAREAVAKTRALDYEA
                     GAVALGRAIHTIQDECAHHGMTNQEHAYYSLTQTCEHADVSPDVQPEAIACAQSRTRD
                     AFVAVAAALQGQRWDYVDNICRDFGADSERDSCASATLPSPAMACDFLAEHDDWDGDD
                     SRWNDRVGPALLAAFQGGLRGDAAPAPLCGTNDKALDPASPRAYVSNREAGCTLIDVT
                     CLGKVDDPGTSDDTQSGGCSTGGTPSSLLLLALLGLRRRR"
     gene            7444..8007
                     /locus_tag="VMZ53_02410"
     CDS             7444..8007
                     /locus_tag="VMZ53_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27323.1"
                     /translation="MPCAICSQIPDQLTANTGREHYLPETTTPLQPLGWGSRDEDLWK
                     CPQCDNLYTYYSEQAFTGSGNNDEDVLTRLTPEQAGVVRAFAIAGTGAADAREEDAFF
                     ALPPLAHSVALSRAYQQNREVVRRLIPRIVAELARTSSFQYRDTLIGLGKARKDDARR
                     IIDEIRKLAVEKGQPLDWVRQQCEASL"
     gene            complement(8004..8699)
                     /locus_tag="VMZ53_02415"
     CDS             complement(8004..8699)
                     /locus_tag="VMZ53_02415"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS27324.1"
                     /translation="MAKRSFFDRRFTAPVRRLLDDWLPPVVREWRPLNTVLARMVHGK
                     NFDLDFKERAFHMSDREIADAYDELAQGGARFRDTDTTPSQLDAIVAAAQGRVLEVGS
                     GNGAVAERLRQAGHAVIAVDVTLGSAEATATRASCPVALAGLPDLPFRDGAFDTVVCA
                     HTLEHIPDLWAAAAELHRVARRVIVVVPRQRYYRYTVDYHLHFFPSAGPLERLLGGTA
                     REVDHDWVVVAER"
     gene            8756..9784
                     /locus_tag="VMZ53_02420"
     CDS             8756..9784
                     /locus_tag="VMZ53_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUS27325.1"
                     /translation="MSATLRIGVIGYGYWGPNLVRNFAAGPRTKVTVIAEQSAHRRQV
                     AASQYPYIRVVDDAAAVLVDSEVDAVAIATPIFTHYELAKRALEHGKHVLVEKPLTRA
                     VAEAEELTQLAAAKKLSLMVDHTFVYTGAVRKIRELVASGELGRILYFDSVRINLGLF
                     QPDFNVIWDLAPHDLTIMDYVLSQTVGANAKWVSAVGASHYGRHENLAYLTVGFEDNL
                     IAHVHVNWVAPVKTRRTIIAGDKKMVVWDDTSPVEPVKVYEAGVDVEHMDKESAYALN
                     VQYRSGDVNSPKLDGKEALSVMAQTFAATCLDGAPSPSDSTVGVRIVRMLEAAQQSLE
                     RQGARIAL"
     gene            9781..10887
                     /locus_tag="VMZ53_02425"
     CDS             9781..10887
                     /locus_tag="VMZ53_02425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HUS27326.1"
                     /translation="MKVPFVDLVAWAKPSREEYLAAFAQIIDTGAYVGGPQVHQFEAE
                     LAAYCGALHAAAVKTGTDALLLALRALGVKPGDEVVTAANSFFATGEAIALAGATPVL
                     ADVDDDTLLIDVDDAARRVTSKTKCIIPVHLFGQLADMDRVHQLGRSHGMRVLEDSAQ
                     AHGASRGNWLAGSCADAAAFSFYPTKNLGALGEGGAVTSSNASVIDEVKRLRDHGQAG
                     RHDHVEVAYNARLDSVQCACLSISLRRLEAANAARRKLASRYREQLTGKAGVRLVTEA
                     PGSKPVYHLMIVRVDAARRDAIRAKMGEAGVATAVHYPTPIHLQRAFAHLGQGPGSCP
                     VAERASTEMISLPMFPDMSDAQVDRVCEVLCASL"
     gene            10910..12295
                     /locus_tag="VMZ53_02430"
     CDS             10910..12295
                     /locus_tag="VMZ53_02430"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS27327.1"
                     /translation="MEKRPHVVVIGGGFGGLEAAKHLASAPVNVTLVDRRNHHLFQPL
                     LYQVATAALNPADIAYPIRAVFANNPNVRVLLAEVTAIDVVAHTVTLDGGELHFDYLV
                     LATGATHSYFGKDQWSTLAPGLKSVEDALEIRRRIFLAYEAAERESDPVAQREWLTFA
                     VVGGGPTGVELAGALGEIGLHTLAKDFRSIDPREVRVVLFEGKDRVLGAYPPKLSEAA
                     KKSLEERHVAVRVNTLVTAIDDRGVTVKGTEGEERIGARTVLWAAGVQASPLAKSLGA
                     PLDRSGRVIVEPDLSIPGHPEVFVVGDLAKMMSNGAEVPGVAQGALQGGRHVAKLIAA
                     DVRAKARNGSRSATTSMARPAFRYHDKGNMATIGRAEAVIATKRVALHGFFAWMLWWI
                     VHIFFLVGFRNRVFMMFHWGWSWLTYKRGSRLITGSVGELPPVRTIAADGTVALPPAG
                     ERISLVEESRN"
     gene            12351..12830
                     /locus_tag="VMZ53_02435"
     CDS             12351..12830
                     /locus_tag="VMZ53_02435"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUS27328.1"
                     /translation="MSDRIQKVTVLKAPRERVWKAISDAKQFGSWFGVEFEGDFTPGA
                     HVTGKMRPTTVDAAVAKMQEQYAGMKFEFWVDSIEPPNRITFRWHPFAIDPKVDYSAE
                     PKTTIVFELADAGDGTKLTITESGFDQIPLHRRAEAFKMNQGGWDAQATLIEKFVAR"
     gene            12827..13150
                     /locus_tag="VMZ53_02440"
     CDS             12827..13150
                     /locus_tag="VMZ53_02440"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUS27329.1"
                     /translation="MRLGNAAAVFAALGDDTRLQLVARLSAGGPQSISRLTDAVDVTR
                     QAVTKHLEALADAGLVRSNKDGRERIWELQPKRLEDAQRYLDEIGSQWDRAIDRLRAF
                     VETDD"
     gene            complement(13152..13661)
                     /locus_tag="VMZ53_02445"
     CDS             complement(13152..13661)
                     /locus_tag="VMZ53_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27330.1"
                     /translation="MGLAATIGGGLFGQFALEPSLDEIATPVERDRMSAKAWNKYSWM
                     KLAGHAAFAIPWFIGRGMLSGNEVSARARALTLAKDILVGASLVTGIASIVIGRTLSK
                     KSREGRGPDQARGQGADNAEAPVLEKTVAAVGIANQLATIGVLAMTSLLAMEGSESMR
                     FAAVTKKLP"
     gene            complement(13739..16168)
                     /locus_tag="VMZ53_02450"
     CDS             complement(13739..16168)
                     /locus_tag="VMZ53_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HUS27331.1"
                     /translation="MRADRYVRWLSRHLTAVLVASAALLGVSVYLAAFHLPLRSDFSN
                     LLPEDAPSVKAAEKLSQRVPARDTMLVLVVAPSPALREVAAKQAIDGIQQIDSDLVHD
                     IEVGNDSTRDFVLAHRHLYIPTKELQQVKDALDKQIADAKLHANPLFIELDDPQPAAD
                     SKQLDDLRAKQREAEAKLKKASHVSADGLTQVIVIHTAFRATDVERDKKLMRSLEAIS
                     NRIRITYPGIEIGYAGGTPVTMAEHSALTRGIVLSSLITLLLVSIVLWLHLRSLRMIA
                     LLALNILIATIIAFGAAALTVGHLNAATAFLGAIIAGNGINYGILLVARYLEERKSAT
                     VESALGTAISGTLLPTLVASLGAAIAYGALGATKFRGFAEFALIGGIGMLICWVCSFV
                     LLPVLVMKYARGLEREPSPLFGRIVVAVFGFRRPVLVTALAGLVTVGAIVISWHYLTH
                     DPYEYDMTQLRSESKDALAARHWLEVSDQNFGRGLAGLAGQTYVAVDDPSQVQPVVDK
                     LQALSDKNPIVGPHSSILDVVPQDQDAKLALLAEIRDQIDLVAEDLPDDQRADLLALR
                     PGDDLRAITVADLPPELASKVTERDGRTGLMIAVKPGAKFDERNGRDLIRFAGAIRSM
                     DGKGVTASGASLLFADVLVQIQKDGPLVTVIAAIGLIAMVLLVVGRSRRAVAVLVASA
                     SGSIAMIAVCALAGLKINFLDFVALPITLGLGIDYAINVADRATRSDPLSALRTTGGS
                     VLVCSLTTMIGYLSLMASENLAIRGFGLASLIGEITCMVAAFVIVPALVALPQWATRS
                     SFDANPASSRA"
     gene            complement(16318..17811)
                     /locus_tag="VMZ53_02455"
     CDS             complement(16318..17811)
                     /locus_tag="VMZ53_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HUS27332.1"
                     /translation="MKRGTVLLVAMAACADNDFVVRPYIDFPANEAATPYPVDQLTIS
                     VAHAGSEIDLVSATFPQGSNISLSDIPFGDDLVMHMTGRYKGSDVAYGRTCQFELRAD
                     ARVPVPHLWLSKTVKWGSLSTQPIARSDGVAITFSDGSGLVLGGHTPELPSEGIRQVE
                     RFDPSTGEYELLHDILPRVGQAVALLGDIADTRVVVIGGVDADTGLGAQFVEVLDADR
                     TTEHQYDMFPDTQMPLVGISATSLTDGRVIAIGGREPPSGAFKQEVIEISVEGGTPII
                     APLRAKLAFPRFLHTATRLGDQVGSPVLITGGLDAAGKPVAQAELFKPLAENFESSFT
                     PAMKKPRWHHQAVRLPDGSVLIIGGVDELGTPIREIELFSLDTGFRLLPDPSNPTLFA
                     KLPDSAGVVDFSATTLPDGRVLIAGGRSFIDGPALNTAFIASLDPFDGTVDIVPTDRL
                     PSDDRGTAVARAGHQATSLCDGTVLIAGGTASQTAFVRYNPPPVSRR"
     gene            complement(17808..18878)
                     /locus_tag="VMZ53_02460"
     CDS             complement(17808..18878)
                     /locus_tag="VMZ53_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27333.1"
                     /translation="MRALGLPLVVAPLAVLVLATASANAAPKADLVVVWAPGMKTAPI
                     EAASKKAGAAFVNTSPPPEASARTGDVVVEGIAAFDALELDRAWQLLEQARSEAVRTG
                     GAGLSRARLSDLFLYRGLVKLQQGDDSSAWEELVASATIDPARELDPGRFAPKVKSEF
                     DRARETVGAKPKVKLKIDAATGCSVYVDGVPSPGTVELPVGSHWVNGSCPDSRPDGYR
                     LELTGDITFPFHPPPYPLPNDSDVLAQARVAGHGLSSFISIEVRSGVATARLVGIDGR
                     ERDRKTVNVDNGDLAPVGDALLALLTPPPPKHWYKSKWAYAGAGAVAAAIVAIPVTLL
                     LTSNTGPTSASSTPDWHGMPPW"
     gene            18939..20711
                     /locus_tag="VMZ53_02465"
     CDS             18939..20711
                     /locus_tag="VMZ53_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27334.1"
                     /translation="MSRALGRYELLRPLARGGMGEVYLARRRAAGVEKWLVVKRMRPE
                     RAGDPRFMDLFKREAMLSMSLAHQNIVPVFDFGRIDDQVFLAMERVEGKDLGSTIQKA
                     GKPLPPLLAAFIAGECCQALHYAHHRKGPDGAALEIVHRDVTPRNVLLSWSGEVKLTD
                     FGIAALAGDDPAKSKMAGTPAYMAPEQARAEPNLDARADVYAVGMVLREALTGERPRR
                     GADREATIAAAKTGVIDPWPEGFESPLKKIVDRATSADKEDRYPDARSMLDDLDVFMV
                     GERAVAKKGDQPARLLAAWLAEVWPGERDESSLPPADAPALEAGELMSFMDDGALDAL
                     GSGTQRSLLATAAEQTPPAGVPIIADAATRSSKSSAGSSKSSAGSSKSSAGDADAIRA
                     PDETQRPRASRWPILVAALMLAAGAAAFAAWPSDKPEKLEPPIAQGSSVAPELPPPVD
                     AAREQAQGSGGGSAEESATQQGSATQQGSATQQGSAQIVITPKNPKIPKQPKVTPDAG
                     TSQTVTEPAGTKRKVLFQTSPNWSEFTVDGGATKYQTLEAIELAPGTHTIHFLGNKYK
                     PADKTITIEVGDADIRKAVKLDEE"
     gene            complement(20719..21813)
                     /locus_tag="VMZ53_02470"
     CDS             complement(20719..21813)
                     /locus_tag="VMZ53_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HUS27335.1"
                     /translation="MLLAQLANDPVATASAAALKYSTDTDEGIARHRRGKSFAYYTPS
                     GKPVRDRATLARIRALAIPPAWTHVWISSDADGHIQATGRDARGRKQYRYHARWSEVR
                     NAVKYHRLVEFCRALPKIRRAVDHDLECRCLCKRKVVATIVSLMERAQLRVGNEEYTR
                     QNGSYGATTLRNRHVKIRGPVLELSYRGKSGIERRIQLRDKKLANIVRQCHDLPGRRL
                     FEYIDEDGNVRPVTSQDVNDYLRDVSTGSFTAKDYRTWAATMAAALLLCALDHPGSDR
                     ACKRCVNAMLARVANQLGHTPSVCRKSYIHPQIIDDFMSNKLAKTLARQLRRQVREVG
                     EHDNLIDVSLLRAVEPAVARYLDLRRRLHA"
     gene            complement(21850..23808)
                     /locus_tag="VMZ53_02475"
     CDS             complement(21850..23808)
                     /locus_tag="VMZ53_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27336.1"
                     /translation="MKRVSAICVAIACALIFYLYWKNDDNAGQPDALRGGGEYLPILD
                     RGDGHMLFLMARSTAVDHDWDFNNDLGAFGDPWGQTRNARGHKQIPHPIGPALVWTPM
                     VWVAQAGAFVANIFDAEIPTHGYTAWHQRFVFLSSVICACLAILLGRRLAVQLLGTQW
                     APTYAAIAILLGTSITYYATNMPSYGHALDAGLCGAFLGTWALTIGQRSWRRWITLGL
                     LLGVAALVRQQDFTLGIVVAVEIVTSVVADVRRRLVDWRIRAITAMAGGALVLLVALI
                     VFIPQLYYWHVVYGDWLSMPQGARYTRFGSPMMLELLYSPRNGWFSTTPLAYAATLGL
                     FTLPKRARLVMIGFLAVVFTQVYLNSTIIDWWGMASFGQRRLCSVTLILVVGLASLIW
                     RCGRLLARLPRVPRAVWHVLAIVLLLPFIAWNLDRVTDLKAGKAAPTELEPTCCAKLP
                     TKRPYIARPIAYVYSWIGNPFEFPANAYFALKHDVEIQRWDVAVGYYALVPPAATLVD
                     DRMYFERGAWRIGYPKAEPYLIGGWSGPHMGSGKSFRWTTDRVVRVIVPNLMPYPQRL
                     RLWLHAGAGQHAIIRWDDRIVARADLQEPTWHAVQFDLYDMPVGEHELSIESDVIAPL
                     PNPPDGWPVPRKPVGVAVNLLELEFIPP"
     gene            complement(23805..24629)
                     /locus_tag="VMZ53_02480"
     CDS             complement(23805..24629)
                     /locus_tag="VMZ53_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUS27337.1"
                     /translation="MDRRGRRRRRSNRDPQCDDGGAQRPSLYDAAGMLGSLSIVVPAF
                     NEEESIEATVDDALRIGATVARELEVLVCNDGSRDDTSAILAAKAAVEPRLRVLDRTQ
                     NAGIEASMRALYANAQHEWVFLMSADRQWPMTALVELAGAAERGADFVVGVRPNKRAV
                     YTPYRRLVSWGYERIVKTFGAPAGDPGSIKLARRELLHTAVAAQGVFAEGERVIRAAR
                     SGARVVEVAVEFHRRAVGKATGARRDVVARALVDAVRVASSLTLGWPRPELPTPDV"
     gene            24561..27140
                     /locus_tag="VMZ53_02485"
     CDS             24561..27140
                     /locus_tag="VMZ53_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUS27338.1"
                     /translation="MRAAVVALGIAIAAPSASAATIHGLVYEDTNADGQPSSGELGIA
                     NAVVAVGASTFAVTDAAGQFDLPVSDTVTTGIVWVRVPDGFRPGPVWAKWDGKADIDL
                     GLRRYTPPRGPLTFVAAADTHIAVTQEYVTAADLALASTTATALEPSPAFFTILGDIT
                     QGNRAQEFVLVDQALSGLDVPWIPVPGNHDWYDGGEAWFAHYGPDNYSFDIGSTHFVV
                     WNMAMSEAEIRVYLGAELSRVPRTMTVVALTHAPPAERVVDALRELGVDYVLTGHAHS
                     NRVVDHEGVIELNTEPFLMGGLDFTPAGYRVMTLEGGRLQSTHHTTVDEPFVEIVSPA
                     AGACTSSSAELIVAAEIEAAPTTISARVDCATPMTLRPAGGWSYRVTLPALEAGPHGV
                     EVTATTTTGTRITRYLAFEVCTPPSPPAVGLDWPQVGGGPSHLGATPHELAPPLATRW
                     TATTGGHVLSAAPVIANNTVYVAATDLADGNTGGISAFDLTTGTLRWRVATPFAVRGG
                     LAVVENTVVAPQIDGIVLGIDATTGTLRWRHELSPEVGTIGSEARASFAPPTTDGDDV
                     LIGHQRSLAALSARTGSPRWTADPVPDGRDSQTASALAIGNGLVVGTFNRAIGGLIAF
                     DLVTGKRVWSYSDHETIAINAAPIIAGDSIFVVSGADQVTAFDLSGQPRWRAQLDEQG
                     FEWGNASLGTPAYAQDILVVPTLYRDLVALDASTGIELWRHAGAPSPLRTTHYRSGGE
                     SGFAASPVITGDIVWAADTSGDLTALDLHTGRLLWHTSLDVPVLAGLAVSGDWLVAAS
                     YDGSVRALVPTTQLRAPVTAPSCTEPPPQAGCCDARGRSAASSLLLIAIVAAALARKR
                     MRR"
     gene            complement(27076..29370)
                     /locus_tag="VMZ53_02490"
     CDS             complement(27076..29370)
                     /locus_tag="VMZ53_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27339.1"
                     /translation="MRRLREWWWVPLLYSLAVIWIYRDLWHQHGVATGLGWDTIDTHG
                     PDLDFFSTEIREGRFSLWNPYDKGGYPIFCDPVFDRYYPLNWPFAVWGAVIGTSWWLV
                     QIKVLAHHALAGIFMHIFLRSRALSHRAAMTGGLGLVACAPLLVHKASNILWPLIWVP
                     LVWLAIDYALARPNWRRGIAIGAALLPPMTAGSPPGLFYAGLLVLPYATWRLVGYLRT
                     RPSRAHLLQLAICIACAVAIAALVLAVTVLPTQELVALGSRDRFSPPGPRFALALSLP
                     FTQVLRGVGVRGAGLPEVYMGAAVMLLSLLALVVRPKFDRGAAIIFALTAALGLWLAA
                     GQTGGLLTLLVYKLPAFDMLRVPGRYKLIAAWAIAASAGYGVAALEAAREETRLRIRA
                     FVVAGVALVLTIVLVYGWANGPSPADRPPWWSIVATAFAGLLAVAGLRAPRKIADAAL
                     AAFALCALLEAPLFTFNKHAQPAAEPRQTHTADAKSLAQLDGARDRWRVYDEFLLGER
                     AGARLRIRDFRGYPAVDPISLHRYVDVLDFAARDASILEDFNVRWLFIKPHFRYVNTT
                     SFPRMPHADFEWKRNFTWEARYPAPLIQWLGQAVVVPKKSVLQTMRGRVGPNGWRYMA
                     VLEPEDQALLPSSASSWVTAGDPTQLGPAPTPVEGTLVSYEPDEIRFTVDAPQEGLVV
                     LNEIMFPGWQVEVDGRATTPVRANYLLRAVVVPQGHHTITWRFEPRRWRALVACYVLA
                     LLAIIGAFVSAQVRLRRSRSVAVS"
BASE COUNT         5178 a         9655 c         9796 g         4782 t
ORIGIN      
        1 aagcgaaagc tcgcggagct cgacttgcag acgcgtacac cgcgccttga cgttcgcgtg
       61 ctcgagccgt gcactccgcg taagtgaagt gcatagggta cgatggtgcg atgaggcgcg
      121 cagtggcctc cattttcgtc atcgtcgcgg gttgcggccg cctctcgttc gacgcacgcg
      181 gcgatacgag tggcagcgcc accgagctcc atgacgcggt gctcatcgcg tcgctctcga
      241 cgaatgtcga cgatgatgat ccagagatct cattcgacgg cctcgagatc tacttctcgc
      301 gcgcgctcgt gacgaacgag gacgacatct atgtatcgat gcgcgcgtcg gtgctcgatg
      361 aatggccagc cgcaacgaag gtgccgggca tcaacacggg cgccatcgat aagggcccgg
      421 gattgacgag tgacgcgttg acgctctact tcggcagcga tcgcggcggc gctccgcaga
      481 agatgtacat gacgacgcgc gcatcccggt cggatccctg gggagcgccc gtgctcgtgc
      541 ccgagctctc gctcgcgcgc aacgtcggca gtccatgcgt gcgtggtgac cgcatcgtgt
      601 tccgccaagc aggcggcacc ggcacgcatg atctgttgca cgcggagctc gtcggtacga
      661 cgtggaatac gccccagctc atcaccgagc tgaacacccc cggagaagac tccggaccgt
      721 tcctgttcgg cgatacgctg tactttgact cgaaccgatc gggcggcgtc ggaagcgagg
      781 acatctacac ggcgcactgg gatgcagcga gcggtacgtt cgagcctgct gtactcgaca
      841 cgacgctgtc gagcacggca caggacaccg acgtctcgct caccgccgac ggcaacacga
      901 tcgtgttctc gcgcaatatc gccggtagcg gcaccggtca cgacatctac accgccacgc
      961 gttagcgcta cttcgggcag acgcgacggc cgatcatgcc gccgaggatc gcgcccggga
     1021 cggcgcccca gccgaagctc tccgatgtca aaatgccgcc gaggaggcca cccgttgctg
     1081 cgtacgagtc gcggcagacg tcgcctgctt cggttccgcc actcactttt tcgagatcgt
     1141 cttgtttgag gtcttgcatg ctcgttcgct gttgcgacgg caatgccagc tacacgagtg
     1201 tcaccgcccg atgcgcaact cgtgaaaatc tgcgacgcgg gagacggtga cgttcactcg
     1261 cggctacggg gtgcggagcg cccgcaacga ggacgcgcgg cctcgctgtc acttcacctc
     1321 agcaggacgt gcccgcgggc atgcagacgt tgtcgccgaa gcacgacgtg cacgtctcac
     1381 ctgcgtcaca gcccgctgtc gcacagtcgt cgccgaagcg ccagagctcg gcccatggct
     1441 gtgaagacga ccagcgtacc cacatgagcg tttcggggat cgtgagatcg ggcgagtact
     1501 cgaggcgatg atgaacaccg ccttcgagta gatcgatgta gtcgtgcccg gattggtgca
     1561 cgagcttgaa tgagccggtg tgcgtcgcgg tgccggagat ttcgtccgcg atcacggcgc
     1621 ggaatgcacc gctcgagagc tcgaggcgcg agacgtcgcc ggcaccgacg tagatgccgc
     1681 tcggtgtgaa tgtgctggcg ctgtcggcct tgcccgtcgc gacatcggct tgcgcgttgt
     1741 cttcggtgtc gtcggcgacg catgcgggga ggagagcgat agagaagaga atggaagcga
     1801 gatacttggt catggtcagg agcctttcgt tgccccttgg agccagacca tcggcagttg
     1861 ttgcagaaaa agtttagcca gtggtttcgc gcagaacgcc ttcgagtgtg acgtcgacgt
     1921 gatcagcgag gagccgcagg atgctctcgc actcggtcgg gctgatcgcg aggcgtgtgc
     1981 ggaggcgctt cttggtcttc gcgatcagcg tgtcgcgcgc actcttgagc caacgcgatg
     2041 cagtggactt gtgagctccg tgcagctcgg cgattgcctc gacgcccaga cgatcgacga
     2101 cgttgtgccg gagcagacgg cgctcgcgcg gctcgagatc ggcgaatgcc tcggcgaacg
     2161 ctgctttgaa gtctgcgtga tagcgcgtgc gaaggtgttg cagctcgaga tccgacgcac
     2221 taccgacgag cgcgtcgagg tcaggctcgt cgagcggagt ctcgcgacgt gcgtcgcgca
     2281 gcacgcggag gcccgcgcgt acggcggtga tcgtgagcca cttgccgagc tcgccgcgac
     2341 cggcgtagtc ggagatgtgc gggcggtcgc cgacgaacag gtgctcgcgc actcgctgca
     2401 tggcttcgtc ggcgagagtc gcatcgagct tgccgcgcac gatgctctcg acgcgcggga
     2461 agtggcggcg ctcgacgacg gcgatggcgg ccacgttgct gttcgcgcac gcgcatgcga
     2521 gatagagatc gcccgcgggc aactctgcta gccacccggc cggagcagtc gccgcgcgtt
     2581 ctgccaagta gcgcatgaac gtgctcgaat cgactgtcac ctctggatag cagacgcgcg
     2641 cttcgtcgac gatttgcgcg agctcgcctt cgagctgcgg cgtatcccat cgcgatcgct
     2701 cggcgggaga cacagcgttg agaaaatccg tgcgcaatgc ggtttcggcc atgggtggtc
     2761 tcgcggctgc atgatgcaag gttcatggca tgcatcggca ccgagacgct tctcgacctc
     2821 gcgtcggata agtcgccagc tcggctctcg cgcttcgagc atcacctcga tgcgtgcgac
     2881 gagtgccggc gcttgatcgc agagctcgcg ggcctatcgt ggatgcgagt cgcgtgcgcg
     2941 cacgacgaga ccgcgacgac agagccgcag cgcggaacgc tcgaggtcgg ccgcgtcatc
     3001 gatcggtaca tcttgatcga tgtcatcggc agcggaggca tgggcatcgt ctacgcggca
     3061 tacgatccac ggctcgatcg caagatcgcc ctcaaggtgc tgcggccgga tcgcgatgga
     3121 gacgagcggc gcgcgaggct gatgcgggag gcacaagcac tcgcgcagct gcggcatccg
     3181 aacgtcgtcg cgatccacga tgcgggcgag acggacggcc aggtgttcgt cgcgatggag
     3241 ctcgtcgagg gcaccacgtt gcgcgagtgg ttgcgcgcgg agcgtcgcga ctggcggggc
     3301 atcatcgcga tgtttctgca agcgctgcac gggctcgcag ctgcacacgc cgccggcatc
     3361 gtgcatcgtg acttcaagcc tgacaacgtt ttgatcggcg acggtcgcgc gcaggtcgcg
     3421 gacttcgggc tcgcgatcac cgcgaccact tccggcgagc ttgccggaac gcccgcgtat
     3481 atgtcgccgg agcaacggcg cggagatgtg ctgacgtccg catccgatca gtacagcttc
     3541 tgcgtcgcgc tcaaagacgc gctctccggg acacgccctc ctgctcgaat caccgctgcg
     3601 ctcgaacgcg gcatggctcc ggcccccgcc gatcgtcatg tctcgatgga ggcgctcgca
     3661 cgcgcgctgc gctggcgctc tcctattcgc atcccagcga tcatcaccgg tgcagcgtgc
     3721 atcgggctcg ccggcttcgc cgcactgtcc tcgactcgca gcgaggcggg tcccacgtgc
     3781 agcgtcgaga gcgcggaagc caagctcgcg agtgtctggg atcgcgagca ggtgaagcag
     3841 ctcgtgctcg cgacgaacag cgcgaacgcg ccggcgatcc tcgcgtcgat cgagcagaag
     3901 gtcgatgcgt acggcaacgc gtggatcgcg gagcgcacgg atgcatgtgc cgccacctat
     3961 gtccgcggcg agcaatcgcc cgagcgcctc gatcagcgaa acgcctgcct cgatacacgc
     4021 cttcaagagc tggcgacgag gatcacggcg ctacgcgata tcagcgcggc caacctcgcc
     4081 accggtccgg cgatcttcga tgatctcgtc tcacccagtg cgtgcagcag tcgcgcttcg
     4141 ctcgcgcacg agtccgcacc accgtcgatg ggtgcagtca gcacgttcga cgacgggagc
     4201 ctggcgtctc gcttcggcgc cggctgggct gtctcgaccg acgtcatcat gggcggccat
     4261 tccacgggca agctctcggt cgtcgacggt ggcgcaaacg gcacgcccaa cgccctcgag
     4321 atcaccggca cggtgtccga aggaggtgcc atcagctggg cgggcgcgat gttctctccc
     4381 ggcgcgagcc cgatgatgcc cgtcgatctg tccgcgcatc acttcgtccg cttcgctgcg
     4441 cgcggtgacg gcggcagcta caacgtcctc ttgttctcgc agacgaaagg cgacctgccc
     4501 gcgttccgcc ccttcgttgc gggcacgacg tggacgcagt atcgctttcc gctcgcggaa
     4561 ttcgatggcg tgaacccagc cgacatcaag ggcctgctct tcgcgagctc gcgccaagga
     4621 ccgttcgcgt tgcagatcga cgagatcgaa ttcgagtaat gtagtgacaa tgcctcaacg
     4681 catctttgcg ctcgcgtttt catctgtact cctcgtcgtc acggtcgctg cttgcaagaa
     4741 ggccgacaag ccggcgccga ccgccgacaa gcaagtgacc gctgcaccag ctggcggcag
     4801 tgccgcggct gccgcgccca cgccggccga gggagctggc gactccgact cgagcagccc
     4861 ggactacacc aagccggcac tcaccgaggc caagatccag ggctacatca aatcgatgaa
     4921 ggacggacac aatccattcg acgtgatcgg cgctgcagcc gggatgatga atgggaaaac
     4981 ctccatcgca aacgcgcagg cgatcgtcga cgagcaggaa gccgtggcga agaaatatgg
     5041 cttcgcgagc tcggaggatt acatggacac ggctggccgc atcatgctcg gcgaggtgac
     5101 gctcggcgcc gcaggcagca tggagcaggt gcgccagatg atggtgcaga gcatcgcgga
     5161 ctacgagaag cagctcgccg acccggcgct gccggcggag acgaaggctt cgctcagcga
     5221 ggcgctcgcg gactccaaga agtcgctcgc ggagatggac gcaagcgcca agagcggcga
     5281 ccagctgaac gccgctgatc tggagctgat caagaagttc aagaccgaga tcgaggcggc
     5341 cgagaaggcg aacgcggaga tccagaacgc caagcacaag tagcgcgcca ccgatggccg
     5401 ggttcctgtt ccgattccgc atggtgccga tcatctcgag catgctcgcg atgcggcgct
     5461 acgatgcgcg cgagctcgtt cgcgaagcgg ggctgcccga cgaggcgttg cagggcgaga
     5521 tcatcgcgcc gttcgttcgt gtgcagaagt ttctcgagct cgcagccgca aagctcgaag
     5581 cgccgttgtt cgggctcgac ctcgcggagc gcattccgac aggcgcattc ggcttgaccg
     5641 agttcttgat gcgcagcgcg ccgagtgtcg agcgatcgct gcaggtgctc atcgagttcg
     5701 gatcgctcgt caacccgatg ctcgatttca gattcacgac gaccgagcac gagggcgtct
     5761 ttcgcttcgc tgtgccttcg caacgcgatg cactcggcat gcacttgaac gagtacacgc
     5821 tgatgctcgt ggcacggcag ttcagcctcg tgcatggcga gcggctgacg ttcgagcgcg
     5881 cgtggtttgc gcacgggcgc aggacgcatg cagaggaagt cgccgcgcga cttggctgca
     5941 gcgtcgcgtt tcaagcggcc gactgcggct tcgcgctctc gcgtgcatgg ctctcgcgca
     6001 gctcgccgat ctccgatggc gcgttgttcg agttcctgct gaaccaagcg cgcacgcagc
     6061 tcgcgaacct cggctcgcgc gacatcgttt cgcaggtcgc gagcgtgatc gagacccggc
     6121 tttcgagcgg tgctctcgac gccggagaca tcgcgtcggc gatggcgacc acgctgcgca
     6181 gcttgcagcg gcatctcgcc gacgccggta catcgtatcg cgaggtcttg cagcatgtgc
     6241 ggcttcggcg gcgcgccgag ctcgcgcaca gcgggctgac accggcggag atcgctcagc
     6301 ggcttggctt ctcgaacgtc acctcgatga agcgctccct cgacgaatcg caagattagt
     6361 caccggctac tcgacgagcc gccgtcgcca aggccgcgaa ttcacgtcca cgcagcgtgg
     6421 catggcgggt gctgttaccg actgcgtcca tgcgcatctg gccactcgtg cttcctatcg
     6481 tgctcgcggc cacgcaagcc tgggcgctcg agccggccaa gcatcgctcg ctcgccgaga
     6541 agtcctgtgc ggacgtcggc ctccccgacg cgtactgccg gcgcatgggc aaagaggtct
     6601 accagaccga ttatcgcgag tggaccgacc tgcacgcgca cgcacagcgt gagcttggcg
     6661 aggatcgctg caccgcggcc gatgctgcgc agtctcgcgt cgatcggctt gctcgcgaag
     6721 ccgtcgcgaa gacgcgcgcg cttgattacg aagccggtgc cgtcgcgctc ggtcgcgcga
     6781 tccacacgat tcaagacgag tgtgcgcatc acggaatgac gaaccaagag cacgcgtact
     6841 actcgttgac gcaaacgtgc gagcacgcag acgtcagccc cgatgtgcag cccgaagcga
     6901 tcgcatgcgc gcaatcacgc acgcgcgatg cgttcgtcgc ggttgccgct gcgttgcagg
     6961 gccagcgctg ggattacgtc gacaatatct gcagagattt tggcgctgac agcgagcgcg
     7021 actcgtgcgc gtccgcaacg ctgccttcgc ctgcgatggc ctgcgacttc ctcgccgagc
     7081 atgatgattg ggacggcgac gactcgcgct ggaacgatcg cgttggcccc gcgctgctcg
     7141 cggcattcca aggcggcctt cgcggcgacg ctgcaccggc cccactctgc ggcacgaacg
     7201 acaaagctct cgatcctgcg tcgccgcgcg cgtacgtgag caatcgcgaa gcaggctgca
     7261 cgctcatcga cgtcacgtgc ctcggcaagg tcgacgatcc aggcacgagc gacgacaccc
     7321 agagtggcgg ctgctcgaca ggcggcacgc cgagctcgct gcttctgctc gcgctgctcg
     7381 gattgcgccg tcgccgctag ctccgtcggc cacggactgg cttctcgtgc gatgctgaag
     7441 cggatgccct gcgcgatctg ctcgcagata ccggaccagc tgactgcgaa taccgggcgc
     7501 gagcactact tgccggagac gacaacgcca ctgcagccgc tcggttgggg cagcagagac
     7561 gaagatctct ggaagtgccc gcagtgcgac aacctctaca cgtactactc cgagcaggca
     7621 tttaccggct cgggcaacaa cgacgaggac gtactcacgc gactgacgcc cgagcaagcg
     7681 ggtgtggtgc gagcgtttgc gatcgccggc accggcgctg ccgatgcgcg tgaagaggac
     7741 gcgttcttcg cgctgccgcc gctcgcgcac tcggtcgcgc tgagtcgcgc ataccagcag
     7801 aatcgcgagg tcgtgcgacg cttgatcccg cgcatcgtcg cggagctcgc gcggacctcg
     7861 agctttcaat atcgcgacac gctgatcggc cttggcaaag cgcgcaagga cgacgcgcgg
     7921 cgcatcatcg acgagatccg caagctcgcc gtcgagaagg ggcagcctct cgattgggtg
     7981 aggcagcagt gcgaagcgtc gctctagcgt tccgcgacga cgacccaatc atgatcgact
     8041 tcgcgcgcag tgcctcctag cagtcgctcg agtgggcccg cgctcgggaa gaaatgcagg
     8101 tggtagtcga ccgtgtagcg gtaatagcgc tggcgcggca cgacgacgat cacgcggcgt
     8161 gcgacgcgat gcagctcggc agcggcggcc catagatcgg gaatgtgctc gagcgtgtgc
     8221 gcgcagacga cggtgtcgaa cgcgccgtct cgaaatggca gatcgggcag acccgcgaga
     8281 gcaactgggc agctcgcgcg cgtcgcagtc gcctctgccg agccgagcgt gacatcgacg
     8341 gcgatcacgg cgtgccccgc ttggcgcaag cgttcggcaa cggcgccatt accgctgccg
     8401 acttcgagca cgcgaccctg cgcggcggcg acgatcgcgt cgagctgact cggcgtggtg
     8461 tcggtatcgc ggaatcgcgc tccgccttgc gcgagctcgt catacgcatc ggcgatttcc
     8521 cgatccgaca tgtggaacgc gcgctctttg aagtcgagat cgaagttctt gccgtgcacc
     8581 atgcgcgcga gcacggtgtt gagcggtcgc cactcgcgga caacgggcgg cagccagtcg
     8641 tcgagaagcc gtctcaccgg cgcggtgaaa cgacgatcga agaagctgcg tttggccacg
     8701 tcggcgggac cgtacacctt gaccggggag gcgcgccatg tgacggtcgc cgacggtgag
     8761 cgccaccctt cgtatcggcg tgatcggtta cggctactgg ggcccgaatc tcgtccgcaa
     8821 cttcgcggcg ggcccacgca ccaaggtcac ggtgatcgcg gagcagtctg cgcatcgtcg
     8881 ccaggtcgct gcgtcgcagt atccgtacat ccgcgtcgtc gatgatgcgg cggcagtgct
     8941 cgtagattcc gaggtcgacg cggtcgcgat cgcgacgccg attttcacgc actacgagct
     9001 cgcgaagcgc gcactcgagc atgggaagca cgtgctcgtc gaaaagccgc tcacgcgcgc
     9061 ggtcgccgaa gccgaggagc tgacccagct cgcagccgcg aagaagctgt cgctgatggt
     9121 cgatcacacg ttcgtgtaca cgggcgctgt gcggaagatt cgcgagctcg tcgcgagcgg
     9181 agagcttggc cgcatcctct atttcgattc ggtgcgcatc aaccttggcc tgttccagcc
     9241 tgacttcaac gtgatatggg atctcgcgcc gcatgatctg acgatcatgg actacgtgct
     9301 gtcgcagacg gtcggcgcga atgcgaagtg ggtgtcggcg gtcggcgcgt cgcactacgg
     9361 gcgccacgag aacctcgcgt atctcacggt cgggttcgag gacaacctca tcgcgcacgt
     9421 ccacgtgaac tgggtcgcac ccgtgaagac gcggcgcacg atcatcgcgg gcgacaagaa
     9481 gatggtcgtg tgggacgaca cgtcgccggt cgagcccgtg aaggtctacg aggcaggcgt
     9541 cgacgtggag cacatggaca aggaatcggc gtacgcgctg aacgtgcagt accgctccgg
     9601 cgacgtgaac tcgcccaagc tcgacggcaa ggaagcgctg tctgtgatgg cacagacgtt
     9661 cgcggcgacg tgtctcgacg gcgcgccatc gccatcggac tcgacggtgg gcgtgcgcat
     9721 cgtccggatg ctcgaggccg cgcaacagtc actcgaacgt cagggagcga ggatcgcgct
     9781 gtgaaagtcc cgttcgtcga tctcgtcgcg tgggcgaaac cgtcgcgcga ggagtatctg
     9841 gccgcgttcg cgcagatcat cgataccggc gcatacgtcg gtggcccgca ggtgcaccag
     9901 ttcgaggcag agctcgcggc gtactgtggt gcgctccacg cagcggccgt gaagaccggc
     9961 accgatgcgt tgctgctcgc attgcgtgca ctcggcgtga agcccggtga cgaggtcgtc
    10021 accgctgcaa actcgttctt cgcgacgggc gaagcgatcg cgctcgcggg cgcaacgccg
    10081 gtgctcgccg atgtcgacga cgacacgctg ctcatcgatg tcgacgacgc cgcgcgccgg
    10141 gtgacgtcga agacgaagtg catcatcccc gtgcatctgt tcggccagct tgccgatatg
    10201 gatcgcgtgc accagctcgg ccgctcgcat ggcatgcgcg tcctcgagga ttcagcgcaa
    10261 gcgcacggtg catcgcgtgg caattggctc gccggctcgt gcgccgatgc cgccgcgttc
    10321 agcttctacc cgacgaagaa cctcggcgcg ctcggtgaag gcggcgctgt cacatcatcg
    10381 aatgcatcgg tgatcgacga ggtgaagcgc ctgcgcgatc atggccaagc gggccgtcac
    10441 gatcacgtcg aagtcgcgta caacgcgcgg ctcgattcgg tgcagtgcgc gtgtctttcg
    10501 atctcgctgc ggcggctcga ggcggcaaac gcggctcgtc gcaagctcgc gtcgcgctat
    10561 cgagagcagc tcacgggcaa ggcgggcgtg cggctcgtga cggaggcgcc cggcagcaag
    10621 cccgtctacc acctcatgat cgtgcgcgtg gacgcggcac gccgcgacgc gattcgcgcg
    10681 aagatgggtg aagcgggtgt cgcgaccgca gttcactatc cgacgccgat ccacctgcag
    10741 agagcgttcg cgcacttggg ccagggtcca ggcagttgcc cggtcgccga acgcgcgtcg
    10801 acggaaatga tctcgctccc gatgtttccg gatatgagcg acgcccaggt cgatcgcgtt
    10861 tgcgaagtgc tctgcgcatc gctctagccc gtgcgatcat ctaaccagca tggagaagcg
    10921 gccacatgtc gtggtcatcg gcggcggatt cggcggcctc gaagccgcga agcatcttgc
    10981 gtccgcgccg gtcaacgtca cgcttgtcga ccggcgtaac catcatctgt tccagcccct
    11041 gctctaccag gtggccacgg cggcgctgaa ccccgccgat atcgcgtatc cgatccgcgc
    11101 ggtgttcgcg aacaacccga acgtgcgcgt gctgctcgca gaggtgaccg cgatcgatgt
    11161 cgtggctcac accgtcacgc tcgacggcgg cgagctccac ttcgattacc tcgtgctcgc
    11221 aaccggcgcg acgcattcgt acttcggcaa agaccagtgg tcgacgctcg cgccggggct
    11281 gaagtccgtc gaagacgcgc tcgagattcg ccgccgcatc ttcctcgctt acgaagccgc
    11341 ggagcgcgag tccgatccgg tcgcgcagcg cgagtggctg acattcgccg tcgtcggcgg
    11401 aggcccgacg ggcgtcgagc tcgccggagc gctcggcgaa atcggcctcc acacgctcgc
    11461 gaaagacttc cgctccatcg acccgcgcga agtgcgcgtc gtgttgttcg aaggcaaaga
    11521 ccgcgtgctc ggcgcgtacc cgccgaagct ctccgaggcc gcgaagaagt cgctcgaaga
    11581 gcggcacgtc gctgtgcgcg tgaacacgct cgtcacggcg atcgacgatc gcggcgtcac
    11641 ggtcaagggc accgaaggag aagagcgcat cggcgcgcgg accgtgctgt gggctgcagg
    11701 cgtgcaggca tcgccgctcg cgaagtcgct cggcgctccg ctcgatcgca gcggtcgcgt
    11761 gatcgtcgag ccggacctgt cgattcccgg ccacccggaa gtgttcgtcg tcggtgacct
    11821 cgcgaagatg atgtcgaatg gcgccgaagt tccgggcgtc gcgcaaggcg cgcttcaagg
    11881 tggtcgtcac gttgcgaagc tgatcgccgc ggacgtccgt gcgaaggctc gcaacggctc
    11941 gcgcagcgcg acgacgtcga tggcccgtcc tgccttccgc tatcacgaca agggcaacat
    12001 ggcgacgatc gggcgcgccg aggcggtcat cgcgacgaag cgggtcgcac tccacggctt
    12061 cttcgcctgg atgctgtggt ggatcgtcca catcttcttc ctcgtcggat tccgcaaccg
    12121 cgtgttcatg atgttccact ggggctggtc gtggctgacg tacaagcgtg gctcgcgcct
    12181 gatcacgggc tcggtcggcg agctgcctcc agttcggacg atcgccgccg atggcaccgt
    12241 cgcgctcccg ccggcaggcg aacgaatctc gctggttgaa gaatcgcgca actaaacggt
    12301 tgcatatctt ctcgggcgcg catagacatt aggcaaccag gaggttgcgt atgtcggatc
    12361 gaatccagaa ggtgacggtg ctaaaagccc cgcgcgagcg cgtgtggaag gccatcagcg
    12421 acgcgaagca gttcgggtcg tggttcggcg tcgagttcga gggcgacttc acgcccggtg
    12481 cacacgtgac gggcaagatg cggccgacga ccgtcgacgc tgccgtcgcg aagatgcaag
    12541 agcagtacgc cggcatgaag ttcgaattct gggtcgactc gatcgagcca ccgaaccgga
    12601 tcacgttccg ctggcacccg ttcgcgatcg atcccaaggt cgactactcc gccgagccga
    12661 agacgacgat cgtgttcgag cttgcagatg ccggcgacgg tacgaagctc acgatcacgg
    12721 agagcggctt cgaccagatt ccgctgcacc gccgcgcgga ggcgttcaag atgaatcaag
    12781 gcgggtggga cgcgcaggcc accctcatcg agaagttcgt cgcgcgatga ggctaggcaa
    12841 tgccgccgcg gtgttcgctg cgctcggcga cgacacgcgg ctccagctcg tggcgcggct
    12901 ctcggcgggc gggccgcagt cgatctcgcg cctgaccgac gcagtcgacg tcacgcgaca
    12961 agccgtgacg aagcatctcg aagcactcgc cgacgcgggc ctcgtgcgca gcaacaaaga
    13021 cggccgcgag cgcatctggg agctgcagcc caagcgcctc gaggacgcgc agcgctacct
    13081 cgacgagata ggctcgcagt gggaccgcgc gatcgatcgc ctgcgcgcct ttgtcgagac
    13141 cgacgactga ctcagggcag cttcttcgtt accgccgcaa agcgcatcga ttcgcttcct
    13201 tccattgcga ggagcgaggt cattgcgagg acgccgatcg tcgcgagttg gttggctatg
    13261 cccaccgcgg ccacagtctt ctcgaggacg ggcgcctcgg cgttgtcagc gccttggcca
    13321 cgcgcttggt cgggcccgcg tccctcgcga gacttcttgc tcagcgtgcg gccgatgacg
    13381 atgctggcga tgcccgttac gagactggcg ccaacgagaa tgtctttggc gagcgtcaac
    13441 gcacgcgcgc gcgcagatac ctcgttcccg ctcagcatgc cgcggccgat gaaccacggg
    13501 atcgcaaacg ccgcatggcc tgcgagcttc atccacgagt acttgttcca ggcctttgct
    13561 gacattcggt cgcgctcgac gggcgtagca atctcgtcta acgaaggctc gagcgcgaac
    13621 tggccaaaca gcccaccgcc gatcgtagcg gcaaggccca cgtcgtgaac gatccaccca
    13681 gcagatgaca gagcagtcat ggaaatcctc ctgcctgccg gtcttgcaac acgcgctcct
    13741 aggcgcgcga cgacgccggg tttgcgtcga acgacgagcg cgtcgcccat tgcggcagcg
    13801 cgacgagcgc tgggacgatc acgaaggccg ccaccatgca cgtgatctcg ccgatgagcg
    13861 acgcgaggcc gaagccgcga atcgcgaggt tctcgctcgc catgagcgac aggtagccga
    13921 tcatcgtcgt cagtgaacag acgaggacgc taccgccggt ggtgcgcaga gcgctcagcg
    13981 gatcggagcg cgtcgcgcgg tcggcgacgt tgatcgcgta gtcgatcccg aggccgagcg
    14041 tgatcggcag tgcgacgaaa tcgaggaagt tgatcttgag gcccgcgagc gcgcagaccg
    14101 cgatcatcgc gatcgagccc gacgccgagg cgacgaggac cgcgaccgca cgacgcgagc
    14161 gaccgaccac gaggagcacc atcgcgatca acccgatggc ggcgatgacg gtgacgagtg
    14221 gcccgtcctt ctggatctgc acgaggacgt cggcaaacag caggctcgcg cccgaggcgg
    14281 tgacgccctt gccgtccatc gagcgaatcg cgcccgcgaa gcggatgagg tcgcggccgt
    14341 tgcgctcgtc gaactttgcg ccgggcttga cggcgatcat gagcccggtg cggccgtcgc
    14401 gctcggtgac cttcgaggcg agctcgggcg ggagatcggc gacggtaatc gctcgcaggt
    14461 cgtcgcccgg gcgaagcgcg aggaggtcgg cgcgctgatc gtcgggaaga tcctcggcga
    14521 cgagatcgat ctggtcgcgg atctcggcga gtagcgcgag cttggcgtct tgatcttgcg
    14581 gaacgacgtc gaggatcgag ctatgcggac cgacgatcgg attcttgtcg ctgagcgcct
    14641 gcagcttgtc gacgacgggc tgtacctgcg acggatcatc gaccgcgacg tacgtctgac
    14701 cggcgaggcc ggcgaggccg cgaccgaagt tctggtcgga cacctcgagc cagtggcgag
    14761 cggcgagcgc gtccttcgat tccgaccgca gctgcgtcat gtcgtactcg tacggatcgt
    14821 gcgtcaggta gtgccacgag atgacgatcg cgccgacggt cacgagaccc gcaagcgccg
    14881 tgacgagcac cgggcggcgg aacccgaaga cggcgacaac gatgcgaccg aacagcggcg
    14941 acggctcgcg ctcgaggcca cgtgcgtact tcatgacgag cacgggcagc agcacgaacg
    15001 agcagaccca gcagatgagc atgccgattc caccgatgag cgcgaactcg gcgaagccgc
    15061 gaaacttcgt cgcgccgagc gcgccgtagg cgatggctgc accgagcgat gcgacgaggg
    15121 taggcagcag cgtgccgctg atcgcggtac cgagtgcact ctcgaccgtg gcgctcttgc
    15181 gctcttccag atagcgcgcg acgagcagga tgccgtagtt gatcccgttg ccggcgatga
    15241 tggcaccgag gaacgcggtc gcagcgttga ggtggccgac ggtgagcgcc gcggcgccga
    15301 acgcgatgat ggtcgcgatc aggatgttca gagcgagcag cgcgatcatg cgaaggctcc
    15361 gcaggtgcag ccacagcacg atcgacacga gcaggagcgt gatgaggctc gagaggacga
    15421 tgccgcgggt cagcgcgctg tgctccgcca tcgtgacggg cgtgccgccc gcatagccga
    15481 tctcgatgcc gggatacgtg atgcggatcc gattgctgat cgcctcgagc gaacgcatga
    15541 gcttcttgtc gcgctcgaca tccgtcgctc gaaacgcggt gtggatgacg atgacctgcg
    15601 tgagcccatc cgcgctcacg tgcgatgcct tcttcagctt cgcctccgct tcgcgctgct
    15661 ttgcgcgcaa gtcgtcgagc tgcttcgaat cggcggccgg ctgcggatca tcgagctcga
    15721 tgaagagcgg gttcgcgtgc agcttcgcat cggcgatctg cttgtcgagc gcgtctttga
    15781 cttgctgcag ctccttcgtc gggatgtaga gatggcggtg cgcgagcacg aagtcgcgcg
    15841 tcgagtcgtt cccgacctcg atgtcgtgga cgagatcgga gtcgatctgc tggatgccgt
    15901 cgatcgcctg cttcgccgcg acttcgcgca acgcgggcga cggcgcaacg acgaggacga
    15961 gcatcgtgtc gcgcgcaggg acgcgctggc tgagcttctc ggcggccttc accgacggcg
    16021 catcttcagg aagcaggttc gagaagtcgg aacgaagtgg caaatgaaag gcagccagat
    16081 acacgctcac gccgagcagc gcggcgctcg cgacgagcac ggccgtcaaa tgccggctca
    16141 gccaacggac ataacgatca gctcgcatcg gccccgagta aggcaaggca catgccgcgg
    16201 tacgccacgt gagattcgcc ggtaagtact ggcagcgtgg tcggccgaag cgtcaaaccc
    16261 tcgacagatc gcggttaacg cctggcaagg actaccgcgt acccgtcgag gtacccgcta
    16321 tcgccgagat accggcggcg ggttgtagcg cacgaacgcc gtctgcgatg ccgtgccacc
    16381 tgcgatgaga acggtgccat cgcacaggct cgtcgcctga tgaccggcgc gcgcaacggc
    16441 cgttccacgg tcgtcggatg gcaatcgatc agtcggcacg atgtcgacgg tgccgtcgaa
    16501 cgggtcgaga gacgcgataa aggctgtgtt gagcgctggg ccatcgatga acgaacgtcc
    16561 gccggcgatg aggacacggc catcaggcag cgttgtcgcc gagaagtcga cgacacccgc
    16621 ggagtcgggg agcttcgcga acagcgtcgg gttcgatgga tcaggtagca gccggaagcc
    16681 cgtgtcgagc gagaacagct cgatctcgcg gatcggcgtg ccgagctcat cgacaccgcc
    16741 aatgatcagt acgctgccgt ccggcaaccg cactgcctgg tgatgccagc gcggcttttt
    16801 catcgccgga gtgaaagagc tttcaaaatt ctcggcgagc ggcttgaaca gctccgcctg
    16861 tgcgacgggc ttgccagcgg catcgagacc gccggtgatg agcaccggtg aaccaacctg
    16921 gtcgccgaga cgcgtggctg tgtggagaaa tcgcggaaac gcgagctttg cgcgcaacgg
    16981 cgcgatgatc ggcgtaccgc cttcgaccga aatttcgatc acttcctgct tgaatgcccc
    17041 gctcggtggc tcgcgtccgc cgatcgcgat tacgcggcca tcggtgagcg acgtggcgct
    17101 gataccgacg agcggcattt gcgtgtccgg gaacatgtcg tactggtgct cggtcgtgcg
    17161 atctgcgtcg aggacttcga cgaactgcgc accgagcccc gtatccgcgt caaccccgcc
    17221 aatcacgacg acgcgggtat ccgcgatatc ccctagcagc gcgacggcct gacccacgcg
    17281 aggcaggatg tcgtgcagca gctcgtactc acccgtgctc ggatcgaatc gctcgacctg
    17341 ccgaattccc tcggaaggga gctcaggcgt atgcccgccg agcacgagac cggatccatc
    17401 ggagaacgtg atggcgacac cgtcgctgcg cgcgatcggt tgcgtcgaca ggctgcccca
    17461 cttcaccgtc ttcgacagcc acagatgcgg caccggcacg cgcgcatcgg cacgcagctc
    17521 gaattggcac gtgcggccgt acgcgacgtc gctgcctttg tagcgaccgg tcatgtgcat
    17581 cacgaggtcg tcgccgaacg gaatgtcgga cagcgagatg ttcgagccct gcgggaacgt
    17641 cgccgatacg agatcgattt ccgatccggc gtgcgcgacg gagattgtca gctgatcgac
    17701 tggatacggc gttgccgctt cgttcgcggg gaagtcgatg tacggcctta cgacgaagtc
    17761 gttgtccgcg catgcggcca tcgcgacgag cagcactgtg ccgcgcttca ccatggcggc
    17821 attccgtgcc agtccggcgt cgacgatgcg ctcgtcggcc cggtgttgct cgtgagcagc
    17881 agcgtcacgg gaatcgcgac gatcgcagcg gccactgcgc ctgcaccggc gtaggcccac
    17941 ttcgacttgt accaatgctt cggcggtggc ggcgtgagca gcgcgagcag cgcatcaccg
    18001 acgggcgcga gatcgccgtt gtcgacgttg acggtcttgc gatcgcgctc gcgtccgtcg
    18061 atgccgacga gccgagccgt tgcaacgccg gaccgcacct cgatcgagat gaacgagctg
    18121 aggccgtggc ccgccacgcg tgcttgcgcg agcacgtcgc tgtcgttcgg cagcggatac
    18181 ggcggtgggt ggaatgggaa cgtgatgtcg ccggtgagct ccaggcgata gccgtcgggc
    18241 cggctgtccg gacacgagcc gttgacccag tgcgagccga ccggaagctc gaccgtaccg
    18301 ggtgatggca cgccgtcgac gtacacgctg cagcccgtgg cggcatcgat cttgagcttc
    18361 acctttggct tcgcgccaac cgtctctcgc gcacgatcga actcgctctt tacctttggt
    18421 gcgaagcgcc cgggatcgag ctcgcgcgct ggatcgatcg tcgccgatgc aacgagctct
    18481 tcccacgcgc tcgagtcgtc tccttgctgc agcttgacga ggccgcgata caagaagagg
    18541 tcgctcaggc gcgcgcgaga gagccctgct ccgccggttc gcacggcttc gcttcgcgct
    18601 tgctcgagca gctgccacgc gcgatcgagc tcgagcgcat cgaacgccgc aatgccttcg
    18661 accacgacgt cgccggttcg cgcactcgcc tccggcggag gtgacgtatt cacgaacgct
    18721 gcgcccgcct tcttcgatgc cgcttcgatc ggcgcggtct tcatgcccgg cgcccacacg
    18781 acgacaaggt ctgccttcgg cgccgcgttc gcagaggccg tcgcgagcac gagcactgcc
    18841 agcggcgcga cgacgagcgg aaggcccaaa gcgcgcacca aaccgtaatt ttcggctggt
    18901 tcggagaaac cgtcaaccgc gcgctatact tcacgcaaat gagccgtgcg ctaggtcgct
    18961 acgagctcct ccgaccgctc gcgcggggcg gcatgggcga ggtctatctg gcccgccggc
    19021 gtgctgcggg tgtcgagaag tggttagtgg tcaagaggat gcggcccgag cgcgctggcg
    19081 acccccgctt catggactta ttcaagcgcg aagcgatgct ctcgatgagc ctcgcccacc
    19141 agaacattgt cccggtcttc gatttcggcc ggatcgacga ccaagtcttc ctggcgatgg
    19201 agcgggtcga aggcaaggac ctcggatcga ccatccaaaa ggcgggcaag ccgctccctc
    19261 cgttgctcgc cgcgttcatc gcaggcgaat gctgccaagc gctgcattac gcgcaccacc
    19321 gcaaaggtcc ggacggcgca gcgctggaaa tcgtccatcg cgatgtcacg ccgcgcaacg
    19381 tgctgctctc gtggtcgggc gaagtgaagc tgaccgactt cggtatcgcg gcccttgcgg
    19441 gtgacgatcc agcgaaatcg aagatggcgg gcacgccggc gtacatggcg cccgagcaag
    19501 cgcgtgccga gccgaacctc gacgcgcgcg ccgatgtcta cgcggtcggc atggtgttgc
    19561 gtgaagcgct gacgggcgaa cgccctcgcc gtggagccga tcgagaagcg acgatcgctg
    19621 cggccaagac cggcgtgatc gatccgtggc ccgaggggtt cgagtcgccg ctcaagaaga
    19681 tcgtcgatcg cgcgacgtcc gccgacaaag aggatcgcta tcccgacgcg cgctcgatgc
    19741 tcgacgatct cgacgtgttc atggtcggcg aacgcgcagt cgccaagaag ggtgatcagc
    19801 ctgcgcgtct tcttgccgca tggctcgcgg aggtatggcc cggtgaacgc gacgagagct
    19861 cgttgcctcc cgccgacgca cccgcgctcg aagctggcga gctgatgagc ttcatggacg
    19921 acggagcgct cgacgcgttg ggctcgggca cacagcgctc gttgctcgcg accgccgcgg
    19981 agcaaacgcc gcccgctggc gtgccgatca tcgcggacgc cgcgacgcga tcatcgaagt
    20041 cgagtgccgg ttcgtcgaag tcgagtgcgg gttcgtcgaa gtcgagtgcc ggcgatgcgg
    20101 acgccatccg cgcacccgac gagacccagc gcccgcgcgc atcgcgatgg ccgatcctcg
    20161 ttgctgcgct gatgctcgcg gctggggcgg cggcgttcgc ggcatggccg agcgacaagc
    20221 cggagaagct ggagccgccg atcgcgcagg gctcgtcggt ggcgccggag cttccgcctc
    20281 cggtcgatgc agcacgagag caggcgcagg gatccggcgg aggctctgcg gaagagtccg
    20341 cgacgcagca gggctccgcc acgcagcagg gctccgccac gcagcaaggc tcggcgcaga
    20401 tcgtgatcac gccgaaaaat ccgaagatac cgaagcagcc gaaggtcacg cccgatgccg
    20461 gcacgtcaca aaccgtcacg gagccggccg gcacgaagcg caaggtcctg ttccagacat
    20521 cgccgaactg gtcggagttc acggtcgatg gcggcgcgac gaagtaccaa acgctcgagg
    20581 ccatcgagct cgcacccggc acgcacacga ttcacttcct cggcaacaag tacaagccgg
    20641 ccgacaagac gatcacgatc gaagtaggcg acgcggacat ccgcaaagcc gtcaagctcg
    20701 acgaggaata gcgacttgct acgcgtgcag gcgacggcgc aagtcgaggt agcgcgcgac
    20761 cgccggctcg acggcacgca gcagcgagac atcgatcagg ttgtcgtgct cgccgacctc
    20821 acgcacctgg cggcggagct gacgcgcgag cgtcttggcg agcttgttcg acatgaagtc
    20881 gtcgatgatt tgcggatgga tatacgactt gcggcagacg gacggcgtgt ggccgagttg
    20941 gttcgcgacg cgcgcgagca tcgcgtttac gcagcgcttg cacgcgcggt cactgcctgg
    21001 atgatcgagc gcgcacagca gtagcgcggc cgccatcgtc gcggcccacg tgcggtagtc
    21061 cttcgccgtg aacgagcccg tgctgacatc gcgcaggtag tcgttgacgt cttgtgacgt
    21121 caccgggcgg acgttgccgt cttcgtcgat gtactcgaac aagcggcgcc ccggcaggtc
    21181 gtgacattgg cggacgatgt tcgcgagctt cttgtcgcgc agctggatgc ggcgctcgat
    21241 gcccgactta ccgcggtatg aaagctcgag gaccgggccg cggatcttga cgtggcggtt
    21301 ccgcagcgtc gtggcgccgt agctgccgtt ctgccgcgtg tactcctcgt tgccgacgcg
    21361 tagctgcgcg cgttccatca gcgagacgat cgtcgcgacg actttgcgtt tgcacagaca
    21421 gcggcattcg agatcgtggt cgacggcgcg gcggatcttg gggagcgcgc ggcagaactc
    21481 gacgagccgg tggtacttca ccgcgttgcg aacttcgctc cagcgcgcgt gatagcggta
    21541 ctgcttgcgc ccgcgtgcgt cgcgacctgt ggcttgaatg tggccgtcgg catcagacga
    21601 aatccacacg tgcgtccatg cgggcggaat ggccagcgcg cggattcgcg cgagtgtggc
    21661 gcggtcgcgc acgggtttcc cgctcggcgt gtagtaagcg aacgacttgc ctcgacgatg
    21721 ccgtgcaatg ccttcatcgg tatcggtcga gtacttcagt gcggcggcgc tcgccgtggc
    21781 aactgggtcg tttgcaagtt gtgcgaggag cacgcggaat gcttcagcat gcgccgtgcc
    21841 atctcgatct cacggtggga tgaattcgag ctcgagcaga tttacagcga caccgacggg
    21901 ctttcgtggc actggccagc cgtccggcgg attcggcagc ggggcgatca cgtcggactc
    21961 gatcgacagc tcgtgctcgc ccaccggcat gtcgtagaga tcgaattgga ccgcgtgcca
    22021 cgtcggctct tggagatcag cgcgcgcgac gatgcgatcg tcccagcgaa tgatcgcgtg
    22081 ctggcccgcg cctgcgtgga gccacagacg caagcgctgt gggtacggca tcaggttcgg
    22141 cacgatgact cgcacgacgc gatcagtggt ccagcgaaac gacttcccgc ttcccatgtg
    22201 tggacctgac cagccgccga tcaagtacgg ctcggctttc ggatagccga tgcgccacgc
    22261 gcctcgttcg aaatacatgc gatcgtcgac gagcgtcgcc gcgggcggga cgagcgcgta
    22321 gtagccgacc gcaacgtccc agcgctggat ctcgacgtcg tgcttcagcg cgaagtacgc
    22381 gttcgccggg aactcgaacg gattgccgat ccaggaatag acgtacgcga tcggacgcgc
    22441 gatgtacgga cgctttgtcg gcagcttcgc gcagcacgtc ggctcgagct ccgtcggcgc
    22501 ggccttgcct gctttgaggt cggtcacgcg atcgaggttc cacgcgatga acggcagcag
    22561 gagcacgatc gcaagcacat gccacaccgc acgcggcact cgcggaagcc gcgcgaggag
    22621 gcgaccgcat cgccagatga gcgaagcgag accgacgacg agaatcagcg tgacgctgca
    22681 cagccggcgc tggccaaacg acgccatgcc ccaccaatcg atgatcgtcg agttgagata
    22741 cacctgcgtg aagacgaccg cgagaaagcc aatcatcacg agacgcgcgc gcttcggcag
    22801 cgtgaacaag ccaagcgttg ccgcgtatgc gagtggcgtc gtcgagaacc aaccgttgcg
    22861 cggcgagtag agcagctcga gcatcatggg cgagccgaag cgcgtgtagc gggcgccttg
    22921 cggcatcgac agccagtcgc catacacgac gtgccagtaa tagagctgcg ggatgaacac
    22981 gatcagcgcg accagcagga cgagcgcgcc acctgccatc gccgtgatcg cgcggatacg
    23041 ccaatcgacc aggcgacgcc gcacgtccgc gacgacgctc gtgacaatct cgaccgcgac
    23101 aacgatacct agcgtgaagt cttgctggcg aacgagcgcc gcaacgccga gcaagaggcc
    23161 gagcgtgatc caccgccgcc acgagcgctg accgatcgtc agcgcccacg tcccgaggaa
    23221 ggcgccgcag aggccggcat ctagcgcgtg gccgtagctc ggcatgttcg tcgcgtagta
    23281 ggtgatcgac gtgccgagca ggatcgcgat cgcggcatac gtcggggccc actgcgtccc
    23341 gagcagctgc accgcgaggc gccgaccgag gaggattgcg agacacgcgc agatcacgct
    23401 cgagagaaag acgaagcgct gatgccacgc ggtgtagccg tgcgtcggga tctcggcgtc
    23461 gaagatgttc gcgacgaacg cgcctgcctg cgcgacccac accatcggcg tccacacgag
    23521 cgcagggccg atcggatgtg gaatctgttt gtggcctcgt gcattgcgcg tctggcccca
    23581 cggatcgccg aatgcgccga ggtcgttatt gaaatcccag tcgtgatcga ccgccgtcga
    23641 gcgcgccatc aggaacaaca tgtggccgtc gccgcgatcg agaatcggca ggtattcgcc
    23701 acccccgcgc agcgcgtcgg gttgccccgc gttgtcgtcg ttcttccaat acagatagaa
    23761 gatcagcgcg cacgcgatcg cgacgcatat cgccgagact cgcttcatac gtcgggcgtc
    23821 ggcagctctg gccgcggcca gccgagcgtc agcgacgagg cgacacgtac cgcatcgacg
    23881 agcgcgcgcg cgaccacgtc acgccgggca ccggtcgcct tgccgacagc acgtcggtga
    23941 aactcgacag cgacctcgac gacacgcgcg cctgatcgcg ccgcacgaat tacgcgctca
    24001 ccttccgcga acacaccctg cgccgcgact gccgtgtgca acagctcgcg tctcgccagc
    24061 ttgatcgagc ccggatcgcc ggcaggcgcg ccgaacgtct tcacgatccg ctcgtagccc
    24121 cacgacacga gccggcgata cggcgtgtac accgcgcgct tgttcggtcg cacgccaacg
    24181 acgaagtcag cgccgcgctc cgctgcaccg gcgagctcga cgagcgccgt catcggccac
    24241 tggcgatccg ccgacatgag gaacacccac tcgtgctgcg cattcgcgta cagcgcccgc
    24301 atcgaggctt cgatacccgc gttctgcgtc cggtcgagca cccgcaggcg cggctccacc
    24361 gcggccttcg ctgcgagaat cgccgacgta tcgtcgcgag agccgtcgtt gcagaccaga
    24421 acttctagct cgcgagcgac ggttgcgccg attctcaacg catcgtcgac agttgcctcg
    24481 atcgattcct cctcgttaaa ggcgggcacg acgatcgaga ggctgccgag cataccggcg
    24541 gcatcatata atgacggccg ttgcgcgccg ccgtcgtcgc attggggatc gcgattgctg
    24601 cgccttcggc gtcggccgcg acgatccatg gcctcgtcta cgaggacacg aacgccgatg
    24661 gccagccgtc ctccggcgag ctcggcatcg cgaacgccgt cgtcgccgtc ggtgcctcga
    24721 cgtttgccgt gacggacgcc gccggccagt tcgacctgcc cgtctcggat accgtgacga
    24781 cgggcatcgt ttgggtccga gttccggacg gcttccggcc gggcccggtc tgggcaaagt
    24841 gggacggcaa agccgacatc gatctcgggc ttcggcgata cacgccacct cgcggcccgc
    24901 tgacatttgt cgccgccgca gacacgcata tcgccgtcac gcaggagtac gtcaccgcgg
    24961 ccgacctcgc gctcgcctcg acgactgcca ccgcgctcga gccgtcgccc gctttcttca
    25021 cgatcctcgg cgacatcacg cagggcaatc gcgcgcagga attcgtgctc gtcgaccagg
    25081 cgctctcggg cctcgacgtt ccgtggatcc ccgttcccgg taaccacgat tggtacgacg
    25141 gcggcgaggc atggttcgca cactacggcc ccgacaatta cagcttcgat atcggcagta
    25201 cgcacttcgt cgtctggaac atggcgatga gcgaagccga aatccgcgtg tatctcggtg
    25261 ccgagctctc tcgtgtgccg cgcacgatga ccgtcgtagc gctcacgcat gcgccaccgg
    25321 ccgaacgcgt cgtcgatgcg ctgcgagagc tcggcgtcga ctacgtgttg accggtcacg
    25381 cgcactcgaa tcgcgtcgtc gatcacgagg gcgtcatcga gctcaacacc gagccgtttc
    25441 tcatgggcgg cctcgacttc acgccggcgg gttatcgcgt catgacgctc gaaggtggcc
    25501 gcctccagtc gacgcaccac acgaccgtcg acgagccgtt cgtcgagatc gtgtcgcccg
    25561 cggccggcgc ctgcacgtcg tcgtccgcgg agctgatcgt cgccgccgag atcgaggccg
    25621 caccgacgac catctctgca cgcgtagatt gcgcgacccc gatgacgctg cgccccgccg
    25681 gcggctggag ctatcgcgtc acgctccccg cgctcgaggc tggtccgcat ggcgtcgagg
    25741 tcaccgcgac cacgaccacc ggcacgcgca tcacgcgcta cctcgccttc gaggtctgca
    25801 cgccaccctc ccctcccgcc gtcggtctcg actggccgca agtcggcggc ggtccgtcgc
    25861 acctcggcgc aactccgcac gagctcgcgc cgccactcgc gacgcgctgg acggcaacaa
    25921 cgggcggcca cgtgctcagc gccgcgccgg tcatcgcgaa caacaccgtc tacgtcgccg
    25981 cgacggatct cgccgacggc aacaccggtg gcatctccgc gttcgacctc accaccggca
    26041 cgctccgctg gcgcgtcgcc acaccgttcg cagtccgcgg cggtctcgct gtcgtcgaga
    26101 atacggtcgt cgcaccgcag atcgacggca tcgtcctcgg catcgacgcg accacaggca
    26161 cactgcgctg gcggcacgag ctcagccccg aagtcggcac gatcggctcc gaagcgcgcg
    26221 catcgttcgc gccccccacg accgacggcg acgacgtcct catcggccat cagcgttcgc
    26281 tcgccgcact ctctgcgcgc acgggctctc cgcgctggac cgccgaccca gtccccgacg
    26341 gccgcgacag ccagactgcg tcagcgctcg cgatcggcaa tggcctcgtc gtcggaacgt
    26401 tcaatcgcgc gatcggtggc ctcatcgcgt tcgacctcgt caccggcaag cgcgtgtgga
    26461 gctactccga tcacgagacg atcgcgatca acgctgcacc catcatcgca ggcgattcga
    26521 tcttcgtcgt cagcggtgct gatcaagtca cggcgttcga tctcagcggc cagcctcgct
    26581 ggcgtgcgca gctcgacgag caaggcttcg aatggggcaa cgcgtcgctc ggcacgcccg
    26641 cctacgcgca agacatcctc gtcgtgccga cgctgtaccg cgacctcgtc gcgctcgacg
    26701 catcgacggg catcgagctc tggcgacacg ctggcgcacc atcgccgctt cgcaccaccc
    26761 actatcgcag cggcggcgag tcgggcttcg cggcatcgcc cgtgatcacc ggcgacatcg
    26821 tctgggccgc cgacacctcc ggcgatctca ccgcgctcga tctccacacc ggccgccttc
    26881 tctggcacac gtcgctcgac gtgcccgtgc tcgccggcct cgccgtcagc ggagactggc
    26941 ttgtcgccgc gtcctacgac ggttcggtgc gcgcgctcgt cccgacgacg cagctccgcg
    27001 cacccgtcac ggcgccgtca tgcaccgagc caccaccgca ggctggctgc tgcgatgcgc
    27061 gcggtcgtag cgccgctagc tcactgctac tgatcgcgat cgtcgcagcc gcacttgcgc
    27121 ggaaacgaat gcgccgatga tagccaggag cgcgagcacg tagcaggcga ccagcgctcg
    27181 ccagcgccga ggctcgaagc gccacgtgat cgtgtgatgg ccctgtggca ccacgacggc
    27241 gcgcagtagg tagttcgctc gcacgggcgt cgtggctcgg ccgtcgacct cgacctgcca
    27301 gccgggaaac atgatctcgt tgagcacgac gaggccttct tgcggcgcat cgaccgtgaa
    27361 gcggatctcg tccggctcgt agctcacgag cgttccttcg acgggcgtgg gcgctggacc
    27421 gagctgcgtc ggatctcccg ccgtgaccca cgacgatgcc gacgacggta ggagcgcctg
    27481 gtcttcgggc tcgagcaccg ccatgtagcg ccacccgttg ggcccgaccc gcccgcgcat
    27541 cgtctgcagc acgctcttct tcggcacgac gacggcttgt ccgagccact ggatcagcgg
    27601 cgccggatag cgtgcctccc atgtgaagtt gcgcttccac tcgaagtcgg catgcggcat
    27661 gcgcggaaac gatgtcgtgt tgacgtagcg gaagtgcggc ttgatgaaga gccaccgcac
    27721 gttgaagtct tcgaggatgc tggcatcgcg cgctgcgaag tcgaggacgt cgacgtagcg
    27781 atgcaggctg atcggatcga cggccgggta gccgcggaag tcgcgaatgc gaagccgtgc
    27841 gccggcgcgt tctccgagga ggaattcgtc gtagacgcgc cagcggtcgc gcgcgccgtc
    27901 gagctgcgcg aggctcttcg catcggccgt gtgcgtctgg cgcggttcgg ccgccggctg
    27961 tgcgtgcttg ttgaacgtga acagcggcgc ttcgaggagc gcgcacagcg cgaacgcggc
    28021 gagggcagcg tcggcgatct tgcgcggtgc gcggagcccg gcgactgcaa gtagccctgc
    28081 gaacgcggtc gcgacgatcg accaccacgg cgggcggtcc gcaggtgatg ggccgttcgc
    28141 ccagccgtac acgagcacga tcgtcagcac gagcgccacg ccggcaacga cgaaggcgcg
    28201 gatgcgcagc cgcgtttcct cgcgtgcagc ttcgagcgca gcgacgccgt agcccgccga
    28261 ggccgcgatc gcccacgccg cgatcagctt gtagcgaccg ggaacgcgca gcatgtcgaa
    28321 cgccggcagc ttgtagacga ggagcgtgag cagcccgccc gtttgaccag ccgcgagcca
    28381 cagcccgagc gcagcggtga gcgcgaaaat gatcgcggcg ccacgatcga acttcgggcg
    28441 cacgacgagt gcgagaagcg acagcagcat gacggccgcg cccatgtaca cctcgggcag
    28501 gcccgcacca cgcacaccga cgccgcgcag cacctgggtg aatggcagcg agagcgcgag
    28561 cgcgaagcgt ggccccggcg gcgagaatcg atcgcgcgag ccgagcgcga cgagctcttg
    28621 cgtcggcagg acagtcacag cgaggacgag cgctgcgatc gcgaccgcgc atgcgatgca
    28681 gatcgcgagc tgcagcaggt gagcgcgcga aggccgcgta cgcagatagc cgacgagccg
    28741 ccacgtcgcg tacggcagca cgagcaggcc ggcgtagaac aggcccggcg gcgagcccgc
    28801 ggtcatcggt ggcaacagcg cggcgccgat cgcgatgcca cgtcgccaat tcggacgcgc
    28861 gagcgcgtag tcgatcgcga gccatacgag cggcacccag atgagcggcc acagaatgtt
    28921 cgacgccttg tgtacgagga gcggcgcgca tgcgacgagg ccgagaccgc cggtcatcgc
    28981 ggcgcgatgc gacagggcgc gcgaccgcaa gaagatgtgc atgaagatgc ccgcgagcgc
    29041 gtggtgcgcg agcactttga tctgcacgag ccaccacgac gtgccgatga cagcgcccca
    29101 gacggcgaac ggccagttca gggggtagta ccgatcgaag accggatcgc agaagatcgg
    29161 atagccgcct ttgtcgtagg ggttccacag cgagaaccgg ccttcgcgga tctccgtcga
    29221 gaaaaagtcg aggtcgggac cgtgcgtgtc gatcgtgtcc cagccaaggc ccgtcgcgac
    29281 accatgttgg tgccacaggt cgcggtagat ccagatcacc gcgagcgagt agagcagcgg
    29341 gacccaccac cattcacgga ggcggcgcac gccgcgagtc tacttgcgtc gtcggcgcag
    29401 cgcgagaagc g
//
LOCUS       DATLBZ010000050        16925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311 k141_40034,
            whole genome shotgun sequence.
ACCESSION   DATLBZ010000050 DATLBZ010000000
VERSION     DATLBZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16925
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40034"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VMZ53_02495"
     CDS             <1..370
                     /locus_tag="VMZ53_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27340.1"
                     /translation="HGTVLDNQKALTVRSAFLQNREYMDVFYREGYTVMEMEGGPYLS
                     ATYEIAEPRRHPSNEIVSLVDQVGFDLGVIHYASDTPYSRRQSLLSKSLSYFGMESTY
                     GCAIAIARRIFSNELARMGG"
     gene            370..1644
                     /locus_tag="VMZ53_02500"
     CDS             370..1644
                     /locus_tag="VMZ53_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HUS27341.1"
                     /translation="MDEVDSLVIGAGVSGLGFANYWRELHPDAKVLVCEADSEPGGYC
                     KTITRDGFVWDYSGHFFHFKHADIEQWIRARMPAQEVRTVERVAKIRFKDRDIDFPFQ
                     THIHQLPKEDFLECLVSLFFRPKDDAPVHSFGEMLYRRLGAGITDKFLRPYNEKLYAT
                     NLDTLDVDAMGRFFPHADIHDIVANMRPGAAKKHGYNATFTYPAGGAIQYIHALLRDL
                     PPGTVACGEPITKIDLAAKIATTPKRTIKYDRIVSSAPLRTLAKICGVEHDASVFTSN
                     QVLVFNLGFDKKGATGIHWMYFPDPAISFYRVGWYDNILDGDRMSLYVEIGAPDGASV
                     DVPGLRERVLDDLRKERIVTDHQLVAEHHVALDPAYVHITRASLAETGRLRQLLADQG
                     VHSVGRYGGWTYCSIEDNLVETRALAHTLSAT"
     gene            complement(1655..3142)
                     /locus_tag="VMZ53_02505"
     CDS             complement(1655..3142)
                     /locus_tag="VMZ53_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27342.1"
                     /translation="MAAALVYRHSLRAHAFDGAQSEVARWEERWEAARACLIGKVAHS
                     AKTREALAIHEMSPDPWEPAKCTKLVAALTRGPAPDSGIDAVEAAWRTLDKAATDAAR
                     AYVLHISPQNTFADDPLPAALDSLDEARTKLRAAASLPALTETAVSLRAVQVVPIELD
                     MQPMTELEVDSLPSARGIVLFGQAGTHDVQAILTTGATPVVAEVGDGALRSVPDLSWG
                     ALVEEQRVRAGMFDPKGLLRTDFDGRGVFETKGAALVPLVIGAHALGTLAVVDDDKLI
                     LAHVANESLERAAPIALAKASDTITPIVSAADVDGRAALVWTSPVGDVGARVMRPGDD
                     GAAIDVYDMPTGPFCLTAREAWAKTPNGVMRFGGDRFVDNTDSEIGDLVGCGVEAALF
                     RDERDPRRFFVCGPTCSNVDIPAGAPRAPALALVGHELVAIMSHSAVLGVWRAGAQPT
                     FYSLPARALPVTAFDMTAMAMSDGTVIDMVVRMRNEYVVLRVPVR"
     gene            3229..4650
                     /locus_tag="VMZ53_02510"
     CDS             3229..4650
                     /locus_tag="VMZ53_02510"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HUS27343.1"
                     /translation="MQIARDAFIDGAWVAGSKRFAVRDPYDDGVIAEVTDCDDATIDR
                     AIDAAARALPGWRRTLAPERGKLLRALADAMLANEKHLAELCTREMGKPLKESAAEVK
                     YAASFLSWFAGEAERIYGETIPAGREGERLAAVREPVGVCGLITPWNFPMAMLTRKLG
                     PALAAGCTIVAKPAAQTPLGSLAIAQLAEAAGFAKGVINIVTASSGGRVGDRLLASPH
                     IRKISFTGSTEVGKLIAKNAADDLKRVSLELGGNAPFVVLADADLDAAVAGALVAKFR
                     NIGQSCVAANRFIVEASVADAFTAKLVDAVKKLKVGRGTDADVDIGPLIDDKAVEKVT
                     RLIEDAVSHGAKKLVGDPPKGRIVPPVVLAGITPKMELWREEIFGPVVAIRIAKDEAD
                     AIAQANDTEYGLVAYVYTRDGARQVRVSEALETGMVGVNTGLVSTAQAPFGGVKHSGI
                     GREGSRHGLDDYTQLKYIATRIA"
     gene            4678..6000
                     /gene="coaBC"
                     /locus_tag="VMZ53_02515"
     CDS             4678..6000
                     /gene="coaBC"
                     /locus_tag="VMZ53_02515"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HUS27344.1"
                     /translation="MLEGPRTDRAPRDPAFALTGAHIVVCVGGGIAAYKAAELARMLD
                     KAGAQVHVAMTPSAQKFVTPMTFQALTRRPVFTDMWSLTEEAAIGHIQLADRADLVIV
                     APATANLISRLANGMANDAVTSLVLATRAPVLLAPSMNVNMWNHPLTRTNLQKLVDVA
                     KYKVVGPGDGFLACRWTGPGRLAEPEDIVEAASHLLSTQDLAGKRIVVSAGPTYEAID
                     PVRFVGNRSSGKMGIALAAAAKRRGADVTLILGPSAVPPPIGVTTNHVETAAQLQWAL
                     ANAGKGADAIIMTAAVADYRPAREAKEKLKRGALGAKTSIELVANPDLLAELGKSRKG
                     RAPLLVGFAAETEDLIENARKKLVAKKVDLIVANDVSEPNAGFAVDTNHVQLVDDEEV
                     IDVPPAPKTEVAHRILDKIAAMLSGQAPRPSRPMSPAERRTTKPSRRG"
     gene            complement(5997..6551)
                     /locus_tag="VMZ53_02520"
     CDS             complement(5997..6551)
                     /locus_tag="VMZ53_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27345.1"
                     /translation="MRLALTILVLLVPGLAHADRTTIVNIGAGIGALGDVDAIEPDGA
                     GGPRVVLAWENAPLPIPAEEGTFDVGLALVPELVGGALIEEDRAEAYVGVGLRGELKM
                     AQNAMGLLRWTTRGAAYVAARALVMGETRDVTYDFGIGQYFAGRTSFTRFGYEIEVLS
                     RPHYEQTDRHFMGFQLLLYVGWAP"
     gene            complement(6635..7204)
                     /locus_tag="VMZ53_02525"
     CDS             complement(6635..7204)
                     /locus_tag="VMZ53_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27346.1"
                     /translation="MRFACLVAVLLVSNVAYAEERSTVVSVGGAFGFLGGTDVMPEDA
                     GQPGGIGAIRATLAWERPSPEYGAIGKSNFSGSLVPELVVGSLFEEERAEGFVGLGLR
                     AELRAAHNHLSPFGWTYKVGGYLVGRALVLGKSQDATYEFGIGEYFPRRAGYTRAGFE
                     LTMAVRPHTMDTDEAQVIGLLSLFAGWAP"
     gene            complement(7323..8582)
                     /gene="glyA"
                     /locus_tag="VMZ53_02530"
     CDS             complement(7323..8582)
                     /gene="glyA"
                     /locus_tag="VMZ53_02530"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HUS27347.1"
                     /translation="MHPALAKTISDVDPEIAELISSEEKRQRDSIRLIASENYVSGAV
                     LAATGSVLTNKYSEGYPGKRYYEGQQFIDKVEQLCIDRAKSLFGVDHANAQPYSGSPA
                     NLAVYFAFLKPGDTVMGMGLPAGGHLTHGWNVSITGAYFKAVQYGVRKDTHRVDLDEV
                     RELARKERPKLIFCGGTAIPRTIEFEQYGSIAKEVGAILCADIAHIAGLVAGGAHPSP
                     VSVADVVSTTTHKTLRGPRGGMLMCKADHAKAIDRAVFPGLQGGPHNHTTAGIAVALK
                     EAAQADFKTYAHQIVANAKALAAAMVERGFSLVTGGTDNHLILADLTSKNVPGKIAAK
                     ALDAAGIELNYNSVPFDPRKPFDPSGIRIGTSAVTSRGMKEAEMRQIAVWMDQVVSNP
                     TDTALHAKVAGEVRELCAKFPAPGILV"
     gene            complement(8595..9179)
                     /locus_tag="VMZ53_02535"
     CDS             complement(8595..9179)
                     /locus_tag="VMZ53_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27348.1"
                     /translation="MMIVGIAALVRNALDGRDFERLLSPPSGRHWHAWQIIVGTWIAA
                     TAAGLLGMLVRDRGDRWRVVSYVVPAAAVALILPLTIHMPIVLYMGGTDAFDGWVQYA
                     GLFVLLAHITFAAMVGARAKAIVEGREPTPVHSIYGITVAMACVPGLIVIIPVIVTAA
                     TGVPILPLLYLMERWGARARLEGEMPMAVARIAS"
     gene            9296..10831
                     /gene="trpE"
                     /locus_tag="VMZ53_02540"
     CDS             9296..10831
                     /gene="trpE"
                     /locus_tag="VMZ53_02540"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HUS27349.1"
                     /translation="MYTPSREEFVAAAARGNLIPVYRELLADSDTPVSAYAALGSGEH
                     SFLLESVVGGSTWAAYSFVGVAPRAVVKWAAGKADVTWYDVDGGGPPKTAQWETADPT
                     SALAEVLGEMRPVEMPGLPRFWGGAVGWIAYDCVRAFEDLPARARPAPGLDDLPPLAM
                     VVTDTLLIFDNLRQTLKVVATPYVSRPERAEVAYDRACARIDAIIATLRAPRRPLPEL
                     EPPRLGERDTPVTPPSSFTHDDFVKAVDAVKEYILAGDAFQVVLSQRFTEPAAGARAL
                     NVYRALRVINPSPYMFHLQFPEACVTGASPETLVRLSDGRVEVRPIAGTRPRGKTAVH
                     DEELATELLADPKERAEHLMLIDLGRNDVGRVAQVGSVHVQEQMVIERYSHVMHMTSH
                     VVGTLATGKSWLDVLRAAFPAGTLSGAPKIRAMEIIDELEPHQRGIYGGAVGYVSYTG
                     NLDLAIAIRTLVSHGDTIYVQAGAGLVADSVPELEYQETVNKARAVLRAVAMARTAKE
                     SQQ"
     gene            10828..11403
                     /locus_tag="VMZ53_02545"
     CDS             10828..11403
                     /locus_tag="VMZ53_02545"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HUS27350.1"
                     /translation="MKVLLIDNYDSFTYNLAQYLGELGADVSVERNDAISLDQIRERK
                     PAAIVISPGPCTPNEAGISMDVIRTFAGLTPILGVCLGHQSIGQVFGGRVVRAPRIMH
                     GKTSKIFHDEKGLFSGIENPFVATRYHSLVIAPETLPDSLEITAKTWEDEIMGVRHKQ
                     HLVEGVQFHPESIMTTAGKKLLANFLAMARG"
     gene            11429..11845
                     /locus_tag="VMZ53_02550"
     CDS             11429..11845
                     /locus_tag="VMZ53_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27351.1"
                     /translation="MKKTFTLAIALSLALVAGCGKKDDTAKKTEATPADKAPAASPSP
                     TAPVAPAPPDKLMDVAETPAGSPKECADARAIYVKIHDCAKLDEKMRAQLVKTWNDMA
                     EQTLKRWGDANEHDRAFIIDTCKKLPETAGMLVQDC"
     gene            11856..12980
                     /gene="trpD"
                     /locus_tag="VMZ53_02555"
     CDS             11856..12980
                     /gene="trpD"
                     /locus_tag="VMZ53_02555"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HUS27352.1"
                     /translation="MTVAIVRGIQRALERQDLSRAEMAEIVGQIMDGQATAAQIGGLL
                     VALRAKGEAVDELVGAAAAMRSRALALQCPSLDRSIDTCGTGGDGSHTVNISTLAAIL
                     IAACGGVVAKHGNRALSSKCGSADVLEALGVGADVEPAVVERSMQVANIGFAFAPRFH
                     AATRHASGPRKELGTRTIFNLLGPLTNPANVRHQVVGVFDRKWCEHVASALGALGLRR
                     AAVVHGEGNIDEIAVRGETHVAIWDEPASVVRTLQLSPRSFGVRECDPSGLAGGDAAF
                     NANVLRKVIAGRDCADDQRYEAVLHAAAMTAALGLELLQESTLDLSRLPVHYERAVSV
                     VRSGEARLVLHKWREVSNATLGESLDNLAALKPSDYIGVE"
     gene            12977..13762
                     /gene="trpC"
                     /locus_tag="VMZ53_02560"
     CDS             12977..13762
                     /gene="trpC"
                     /locus_tag="VMZ53_02560"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HUS27353.1"
                     /translation="MTSGRADILEKILAVKRDEIAALKVMALADAPARGHRSLRAALH
                     RSPDQPMRVLAEVKRASPSAGPIRADADPVAIAREYADAGAAAISVLTDQQFFDGDIA
                     FLQPCHDAVDVPILRKDFLIDPLQVQEAAAHGADAVLLIVAALSSSELEEMMAAVHEH
                     KLEVLVEVHDMDELDTALSLSRPPTVIGVNHRDLRTFTIDMTLTEQVAKRVPSNIVLV
                     AESGIRTADDVRRMRDAGAHAVLVGEHLMRAPSPGAALRELLA"
     gene            13824..14462
                     /locus_tag="VMZ53_02565"
     CDS             13824..14462
                     /locus_tag="VMZ53_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HUS27354.1"
                     /translation="MTRIKVCGVTLPDDAARVAAAGADFIGFNFWPKSKRYVAPERAP
                     MLAAVARGSGAAKLVGVFVDPDIDEVLAITTRVDIDIIQLHGDETPDFVKRLSLSVYR
                     PVWKAIPVASSKDIQHLDIWSSEAILLDAPTPGRGGAGAKFDWNLAREARERFPRINI
                     ILAGGLNPENVTTAIQTVEPWAVDVASGIEAGPGIKDPAKLEAFVGAARLIG"
     gene            14511..15743
                     /gene="trpB"
                     /locus_tag="VMZ53_02570"
     CDS             14511..15743
                     /gene="trpB"
                     /locus_tag="VMZ53_02570"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HUS27355.1"
                     /translation="MSTADEIARLPEPVNGRFGPFGGQYVAETLMPAIAELEHAWRTA
                     RTDATFWTEVDALLADYVGRPSRLYHAQRLSAAVGAQVYLKREDLNHTGAHKINNCIG
                     QAVLARRMGKRRLIAETGAGQHGVATATVAALFGLPCEIYMGAEDVVRQSLNVERMKL
                     LGAQVHAVSSGTRTLKDAMNEALRDWVTNVGDTFYLIGSVAGPHPYPWLVRDLQSVIG
                     REAHAQILAQAGRLPDALIACVGGGSNAAGLFAPFVATPGIRLFGIEAAGEGVASGRH
                     AATLSAGRVGVLHGSKSYVLTHTDDAKLGQIAHAHSISAGLDYPGVGPEHAHWKESGA
                     VTYLSRTDDDALAALERLARTEGILCALETAHAVAAIDEVAKTIGKPDPIIIVGLSGR
                     GDKDMVTIAQRRHAGGAA"
     gene            16046..16840
                     /gene="trpA"
                     /locus_tag="VMZ53_02575"
     CDS             16046..16840
                     /gene="trpA"
                     /locus_tag="VMZ53_02575"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HUS27356.1"
                     /translation="MTRLRAAFDRAAAEKRAALVAYMTFADPDPSTSVAVVEAACRAG
                     ADVIELGVPFSDPSADGPSIQRAMERALDAGGSLPGAIAAVAELRKRGITTPIVLFGY
                     YNPIFVMGPALFAERAAKAGVDAVLTVDLPIDELAELAQPLAAHGVQVIPLVAPTSTP
                     ERIARLGAVNAPFVYYISLTGVTGGRAAAPVDPARLALIRTAASAPVAVGFGIRTPAD
                     AARFAAIADGVVVGTAIVDQVAAGDAAGAPDRVAKLVGDLARAMPR"
BASE COUNT         2914 a         5880 c         5441 g         2690 t
ORIGIN      
        1 gcacggcacc gtcctcgata atcagaaggc gctgaccgtg cgctcggcgt tcttgcagaa
       61 ccgcgagtac atggacgtct tctatcgcga gggctacacg gtgatggaga tggaaggtgg
      121 cccgtacctc tcggcgacgt acgagattgc cgagccacgg cgccacccga gcaacgagat
      181 cgtgtcgctc gtcgatcaag tcggcttcga tctcggcgtc atccactacg cgtcggatac
      241 gccgtactcg cgacgccaga gcttgctctc caagtcgctg tcgtacttcg gcatggagtc
      301 gacgtacggc tgcgcgatcg cgatcgctcg tcgcatcttc tcgaacgagc tggcgcgcat
      361 gggtggctag tggacgaggt tgattcgctc gtcatcggtg caggcgtcag cggcctcggg
      421 ttcgcgaact actggcgcga gctgcatccc gacgcaaaag tcctcgtctg cgaagcggac
      481 agcgagcccg gcggctactg caagacgatc acgcgcgacg gcttcgtctg ggactactcg
      541 gggcacttct tccacttcaa gcacgctgac atcgaacagt ggatccgcgc ccggatgccg
      601 gcgcaggaag tccgcaccgt cgagcgcgtc gcgaagatcc gcttcaagga tcgcgacatc
      661 gacttcccgt ttcagacgca catccatcag ctgccgaagg aagacttcct cgagtgcctc
      721 gtgtcgctgt tcttccgccc aaaggacgac gcgccggtgc acagcttcgg cgagatgctg
      781 taccgccggc tcggcgctgg catcaccgac aagttcctgc gcccgtacaa cgagaagctc
      841 tacgcgacga acctcgacac gctcgacgtc gacgcgatgg ggcgcttctt cccacacgcc
      901 gacatccacg acatcgtcgc gaacatgcgc cccggtgcgg cgaagaagca cggctacaac
      961 gcgacgttca cgtatccggc aggcggcgcg atccagtaca tccacgcgct gctgcgtgat
     1021 ttgccgccgg gaaccgtcgc gtgcggtgag cccatcacga agatcgatct cgcggcgaag
     1081 atcgcgacca cgccgaagcg cacgatcaag tacgaccgca tcgtcagctc cgccccgctg
     1141 cgcacgctcg cgaagatctg cggcgtcgag cacgacgcga gcgtgttcac gtcgaaccaa
     1201 gtcctcgtgt tcaacctcgg cttcgacaag aagggcgcga ccggcatcca ttggatgtac
     1261 ttcccggatc ccgcgatctc gttctatcgc gtcggctggt acgacaatat tctcgacggt
     1321 gaccgcatga gcctctacgt cgagatcggt gcgcccgatg gtgcatcggt cgacgtgccg
     1381 ggcttgcgcg agcgcgtgct cgacgaccta cgcaaagaac gcatcgtcac ggaccatcag
     1441 ctcgtcgccg agcatcacgt cgcgctcgat ccggcgtacg tccacatcac ccgcgcctcg
     1501 ctcgcggaga cggggcgcct gcgccagctg ctcgctgatc agggcgtgca ctccgttggt
     1561 cgctacggcg gctggaccta ctgctcgatc gaggacaacc tcgtcgagac gcgcgcgctg
     1621 gctcacacgc tgtccgctac gtgacggcat cccgctagcg gaccggcacg cgaagcacga
     1681 cgtattcgtt ccgcatgcgg acgaccatat cgatgacggt gccatcgctc atcgccattg
     1741 ctgtcatatc gaaagcggtg accggcaacg cacgcgcggg gaggctgtag aacgtcggtt
     1801 gggctcctgc gcgccaaacg ccgaggacag cgctatgcga catgatcgcg acgagctcgt
     1861 ggccaacgag ggcgagcgcc ggagcgcgcg gagcaccggc gggtatatcg acgttgctgc
     1921 acgtcgggcc gcagacgaag aaccggcgag ggtcccgctc gtcgcgaaag agtgctgctt
     1981 cgacgccgca accaacgaga tccccgatct cggaatccgt gttgtcgacg aaccggtcac
     2041 cgccgaatcg catcacgccg ttcggagtct ttgcccacgc ttcgcgagcg gttaggcaga
     2101 acggtcccgt gggcatgtcg tagacgtcga tggcagcgcc atcgtcgccc ggtcgcatga
     2161 cacgagcgcc gacatccccg accggcgacg tccacacgag cgctgcacgt ccgtcgacgt
     2221 cggccgccga cacgatcggt gtgatcgtat cggacgcttt cgcgagcgcg atgggcgcgg
     2281 ccctctcgag gctctcattc gcgacgtgcg caaggatgag cttgtcgtcg tcgacgaccg
     2341 ccagcgtgcc gagcgcgtgc gcgccgatca ccaagggcac gagcgctgcg cccttcgtct
     2401 cgaagacacc acgaccatcg aagtcggtgc gcaagaggcc cttcggatcg aacatacccg
     2461 cgcgtacgcg ctgctcttcg acgagcgcgc cccagctgag atccggaacg cttcgcagtg
     2521 cgccatcgcc aacctccgcg accaccggcg tcgcgcccgt cgtcaggatc gcttgaacgt
     2581 cgtgcgttcc cgcctgcccg aacagcacga tgccgcgtgc gcttggcagc gagtcgacct
     2641 cgagctcggt catcggctgc atgtcgagct cgatggggac tacctgcacg gcgcgcagcg
     2701 acaccgctgt ctcggtgagc gcgggcaggc tcgccgcggc cctgagtttc gtgcgcgctt
     2761 catcgagcga gtccagcgca gctggcagcg ggtcatccgc gaatgtgttc tgcggcgaaa
     2821 tgtggagcac gtaggcccgc gcggcgtcgg tcgcggcttt gtcgagcgtg cgccatgccg
     2881 cttcgacggc atcgatgccc gagtctggcg ccggtccgcg ggtgagggca gcgacgagct
     2941 tcgtgcactt cgcaggttcc cacgggtcgg gcgacatctc gtgaatcgcg agagcctcgc
     3001 gcgtcttcgc cgagtgggcg accttcccga tcaagcacgc gcgcgcggcc tcccagcgtt
     3061 cctcccagcg cgcgacctcg ctctgggctc cgtcgaacgc gtgcgcgcgg aggctgtgcc
     3121 ggtacacgag agccgcggcc acgaggacga ccgcaatcag agcggcgatc gctagtcgac
     3181 cgcgcgcgac catgcgcctc tataccattc ccgcgctatg aatcggccat gcagatcgct
     3241 cgtgatgcgt tcatcgatgg cgcgtgggtc gctggctcga agcggttcgc tgtccgcgat
     3301 ccgtacgacg atggcgtcat cgccgaggtg accgactgcg acgatgcgac gatcgatcgc
     3361 gcgatcgatg cagctgcgcg cgcgctgccc ggttggcgca ggacgctcgc gcccgagcgc
     3421 ggcaagctgc tgcgtgcgct cgccgatgcg atgctcgcga acgagaagca cctcgccgag
     3481 ctgtgcacgc gcgagatggg caagccgctg aaggaatccg ccgccgaggt gaagtacgcg
     3541 gcgagcttct tgtcgtggtt cgccggcgaa gccgagcgca tctatggcga aacgattccg
     3601 gccgggcgcg agggcgagcg acttgctgcg gtgcgcgagc ccgttggagt gtgcgggctc
     3661 atcacgccgt ggaacttccc gatggcgatg ctgacgcgca agctcgggcc cgcgctcgcg
     3721 gcgggttgca cgatcgtcgc gaagcctgcc gcgcagacgc cactcggctc actcgccatc
     3781 gcgcagcttg cagaagccgc tggcttcgcg aagggcgtca tcaacatcgt caccgcatcg
     3841 agcggcggtc gcgtcggtga tcgactgctc gcatcgccgc acatccgaaa gatcagcttc
     3901 accggctcga cggaagtcgg caagctcatc gcgaaaaacg cggcggacga tctcaagcgc
     3961 gtctcgctcg agctcggtgg caacgcgccg ttcgtcgtgc tcgcggacgc cgacctcgat
     4021 gcggccgtcg ccggtgcact cgtcgcgaag ttccgcaaca tcggccagtc gtgcgtcgct
     4081 gcgaaccgct tcatcgtcga agcgagcgtc gcggacgcat tcacggcgaa gctcgtcgac
     4141 gcagtgaaga agctgaaggt cgggcgcggc acggacgccg acgtcgatat cggcccgctc
     4201 atcgacgaca aggccgtcga gaaagtgacg cgtctcatcg aggacgccgt tagccacggt
     4261 gcaaagaagc tggtcggcga tccgcccaag ggccgcatcg tgccgccggt cgtcctcgcc
     4321 gggattaccc cgaagatgga gctgtggcgc gaagagatct ttgggcccgt cgtcgcgatt
     4381 cgcattgcga aagacgaagc cgatgcgatc gcgcaggcaa acgacaccga gtacgggctc
     4441 gtcgcgtacg tgtacacgcg cgatggtgcg cgtcaggtgc gcgtgagcga agcactcgag
     4501 accggcatgg tcggcgtgaa caccggcctc gtctcgacgg cgcaggcgcc gttcggcggc
     4561 gtgaagcact cgggcatcgg tcgcgaaggc tcacgtcacg gtctcgacga ctacacgcag
     4621 ctgaaataca tcgccacgcg catcgcgtga tcgaagggcg gggtagagtc cgccgtgatg
     4681 ttggaaggtc ctcgtactga tcgtgctcct cgtgatcccg cgttcgcgct gacgggtgcg
     4741 catatcgtcg tctgcgtcgg cggtggaatc gccgcctaca aggccgcgga gctcgcgcgc
     4801 atgctcgaca aggccggcgc gcaggtgcac gtcgcgatga cgccgagcgc acagaagttc
     4861 gtcacgccga tgacgttcca agcgctgacg cggcgcccgg tcttcacgga catgtggtcg
     4921 ctcaccgagg aagcggcgat cggacatatc cagctcgcgg accgcgcaga cctcgtgatc
     4981 gtcgcaccgg cgaccgcgaa tctcatttcg cgtctcgcga acggcatggc gaacgacgcc
     5041 gtcacctctc tcgtgctcgc cactcgcgcg cccgtgctgc tcgcgccgtc gatgaacgtc
     5101 aatatgtgga atcacccgct gacgcgcacg aacttgcaga agctcgtcga cgttgcgaag
     5161 tacaaggtcg tcggcccggg ggatggcttc ctcgcatgcc gctggactgg ccccgggcgt
     5221 ctcgcggagc ccgaggacat cgtcgaggcg gcgtcgcact tgctgtcgac ccaggacctc
     5281 gcgggcaagc gcatcgtggt ctcggccggc cccacgtacg aagcgatcga tccagtccgc
     5341 ttcgtgggta accgcagctc cggcaagatg ggcatcgccc tggcggccgc tgcgaagcgc
     5401 cgtggtgccg atgtcacgct catcctcggc ccgtcggcag tgcctcctcc gatcggcgtg
     5461 acgacaaacc acgtcgagac cgctgcacaa ctgcaatggg cgctcgcgaa tgcgggcaaa
     5521 ggcgctgacg cgatcatcat gacggccgcg gtcgccgact atcgtcccgc gcgcgaagcg
     5581 aaggagaagc tcaagcgcgg cgcgctcggc gcgaagacgt cgatcgagct cgtcgcgaac
     5641 cccgatctac tcgcggagct cggcaagtcg cgcaagggca gggcgccgtt gctcgtcggc
     5701 tttgctgcgg agaccgagga tctcatcgag aacgcacgca agaagctcgt cgccaagaag
     5761 gtcgacctca tcgtcgcaaa cgatgtgagc gagcccaatg caggcttcgc cgtcgacacg
     5821 aatcacgtgc agctcgtcga cgacgaagag gtgatcgacg tgccgccggc gccgaagaca
     5881 gaggtcgcgc atcgcatcct cgacaagatc gcggcgatgc tctccggcca ggcaccgagg
     5941 ccttcgcggc ccatgtcacc ggcagagcgc cgtacgacaa agcccagccg tcgcggctag
     6001 ggcgcccagc cgacgtacag caacagctga aagcccatga agtggcgatc ggtctgctcg
     6061 taatgcgggc gcgagagcac ttcgatctcg tagccgaagc gcgtgaagct cgtgcggccc
     6121 gcgaagtatt ggccgatgcc gaagtcgtac gtgacgtcgc gagtctcgcc catcacgagc
     6181 gcacgcgccg cgacgtaggc agcgccgcgc gtcgtccacc gcagaaggcc catcgcgttc
     6241 tgcgccatct tgagctcgcc gcgcagaccc acgccgacat acgcctccgc gcgatcctcc
     6301 tcgatcagcg cgccgccgac cagctcgggc acgagcgcga gtccgacgtc gaacgtgcct
     6361 tcctcggcag gaattggcag cggcgcgttt tcccacgcga gcactacgcg cgggccaccc
     6421 gcaccatcgg gctcgatcgc gtcgacatcg ccgagtgcgc cgattcctgc gccgatgttc
     6481 acgatcgtcg tgcgatccgc gtgcgccaac ccgggaacga gcaacacgag gatcgtgagg
     6541 gccagccgca tcgttcgatc atgcatgcgc cggagatcgc cgcgaagtcc gaccgctggc
     6601 aaacgctggc cgggcgagaa ctagatcaca tgtctcaggg agcccagccg gcgaagagcg
     6661 agagcaaccc gatgacctgg gcctcgtcgg tgtccatcgt gtgcgggcgc accgccatgg
     6721 tcagctcgaa ccccgcgcgt gtgtagccag ctcgcctcgg gaagtattcg ccgatgccga
     6781 attcataggt cgcatcttgt gacttgccga ggacgagcgc gcgaccgacg aggtagccgc
     6841 cgaccttgta cgtccagccg aatgggctca ggtggttgtg ggccgcgcga agctcggcgc
     6901 gcagaccgag gccgacaaag ccttccgccc gctcttcctc gaacagcgac ccgacgacga
     6961 gctcgggcac cagcgagccg ctgaagttcg attttccgat cgcgccgtat tcgggcgaag
     7021 gtctctccca cgcaagcgtt gcgcggatcg cgccgatgcc accgggttgc cccgcatctt
     7081 cgggcatgac gtccgtgccg ccgagaaagc cgaacgcgcc tccgacggac acgaccgtcg
     7141 agcgctcttc cgcatacgcg acattcgaga cgagcagcac ggcgacgaga caggcgaagc
     7201 gcatggcccg agcgtgcatc agctgcacgg gcggttcacg ccaatgtcgg cggaatttgc
     7261 gccacatccg cacaatgccg gacgccgtga cgcgcgacgc ccattgcgca cgacgcgcaa
     7321 cgctagacga ggatgccggg cgccgggaac ttcgcgcaga gctcacgcac ctcgccggcg
     7381 accttcgcgt ggagcgcggt gtcggtcggg ttcgacacga cctgatccat ccagacggcg
     7441 atctggcgca tctcggcttc cttcatgccg cggctcgtaa cggccgacgt gccgatgcga
     7501 atgcccgatg gatcgaacgg cttgcgcgga tcgaacggaa ccgagttgta gttgagctcg
     7561 atgcccgcag cgtcgagcgc cttcgcggcg atcttgccag ggacgttttt gctcgtgagg
     7621 tccgcgagga tgaggtgatt gtcggtgccg cccgtaacga gcgagaagcc gcgctcgacc
     7681 atcgccgctg cgagggcctt cgcgttcgcg acgatctggt gcgcgtacgt cttgaagtca
     7741 gcctgcgcgg cttccttcag cgcgaccgcg atgcccgctg tcgtgtggtt gtgcgggccg
     7801 ccctgcaggc cggggaacac cgcgcgatcg atcgccttcg cgtgatccgc tttgcacatc
     7861 agcatgccgc cgcgcggacc gcggagcgtc ttgtgcgtcg tcgtcgacac gacgtctgcg
     7921 accgagacgg gcgatggatg cgcgccaccc gcgacgagac cggcgatatg cgcgatgtcc
     7981 gcgcagagga tcgcgccgac ttctttcgcg atcgagccgt actgctcgaa ctcgatcgtg
     8041 cgtgggatcg ccgtgccgcc gcagaagatg agcttcgggc gctccttgcg ggcgagctcc
     8101 cgaacttcgt cgaggtcgac gcgatgcgtg tccttgcgga cgccgtattg cacggccttg
     8161 aagtacgcac cggtgatcga cacgttccag ccgtgcgtga ggtgaccgcc ggcgggcagg
     8221 cccatgccca tcacggtgtc gccgggcttc aagaacgcga agtacacggc gaggtttgcc
     8281 ggcgagcctg agtacggctg cgcgttcgcg tgatcgacgc cgaacagtga cttcgcgcga
     8341 tcgatgcaga gctgctcgac tttgtcgatg aactgctgac cttcgtagta gcgcttgccc
     8401 ggatagcctt cgctgtactt gttcgtgagc accgagccgg ttgccgcgag cacggcgccc
     8461 gagacgtagt tctccgacgc gatcaggcga atcgaatcgc gctggcgctt ctcttcggag
     8521 ctgatcagct cggcaatctc ggggtcgacg tcggagatcg tcttcgcgag ggcggggtgc
     8581 atgcggcagt catatcacga cgcgattcgg gcgaccgcca tcggcatctc gccctcgaga
     8641 cgcgcgcgtg cgccccaccg ttccatcaga taaaggagtg gaagaatcgg aactcccgtc
     8701 gctgcggtca cgatgaccgg aatgatgacg atgaggccgg gcacgcaagc catggccacg
     8761 gtgatgccgt aaatactgtg taccggtgtg ggctcgcggc cctcgacgat cgccttcgcg
     8821 cgtgcaccga ccatcgcggc gaacgtgatg tgcgcgagca gtacgaagag accagcgtat
     8881 tggacccatc cgtcgaacgc gtctgtcccg cccatgtaaa ggacgatcgg catatggatc
     8941 gtcagcggca ggatcaatgc gacagccgct gccggcacga cgtagctcac gacgcgccaa
     9001 cgatcgccgc gatctcgcac gagcattccc agcagtccgg ccgccgttgc cgcgatccat
     9061 gtcccaacga tgatctgcca cgcgtgccag tgacggccgc tcggaggaga gagcaagcgc
     9121 tcgaagtcgc gaccgtcgag cgcgttgcga accagcgccg cgataccgac gatcatcacg
     9181 atgccaaacg ctaagcgaga tacgcgggtc acagcgaacc tcctgtagat cgggcgggac
     9241 ttcacacccc ttcgatgccg ccttgctcca tcccgatgcg ctataaaggc gtcgcatgta
     9301 cacgccgagc cgcgaggagt tcgtcgcggc agccgcgcga gggaacctca tcccggtcta
     9361 tcgcgagctg ctcgccgata gcgacacgcc ggtctctgcg tacgcagctc tcgggtcggg
     9421 tgagcactcg ttcctcttgg agtcggtcgt cggtggcagc acgtgggccg cgtactcgtt
     9481 cgtcggtgtc gcgccgcgcg cggtcgtgaa gtgggcggca gggaaggcgg acgtcacttg
     9541 gtacgacgtt gacggcggcg gtccaccgaa gaccgcgcaa tgggaaaccg ccgatccgac
     9601 atcggcgctc gcagaagtgc tcggcgagat gcgccccgtc gagatgccgg gcttgccgcg
     9661 cttttggggc ggcgcggttg gctggatcgc gtacgactgc gtgcgcgcgt tcgaagacct
     9721 tcccgctcgc gcgcggcctg caccagggct cgacgatctg ccgccgctcg cgatggtcgt
     9781 caccgatacg ctgctgatct tcgacaacct ccgccagacg ctcaaggtcg tcgcgacgcc
     9841 gtacgtctct cgccccgagc gcgccgaagt cgcctacgac cgcgcgtgtg cccgcatcga
     9901 cgcgatcatc gcaacgcttc gcgcgccgcg tcgtccgttg cccgagctcg agccgccgcg
     9961 ccttggcgaa cgcgacacgc cggtgacgcc gccgtcgtcc ttcacgcacg atgacttcgt
    10021 caaggccgtc gacgccgtga aggagtacat cctcgccggc gatgcattcc aagtcgtact
    10081 ctcgcagcgg ttcacggagc ctgctgcagg cgcccgcgcg ttgaacgtct atcgcgcgct
    10141 acgcgtgatc aatccgtcgc cgtacatgtt ccatctgcaa tttcccgaag cgtgcgtcac
    10201 gggcgcgtcg ccagaaacgc ttgtccggct ttcggacggt cgggttgaag tgcgcccgat
    10261 cgccggcacg cggcctcgcg gcaagacggc tgtccatgat gaagagctcg cgacggagct
    10321 gctcgccgat ccgaaggagc gcgccgaaca cctcatgctg atcgacctcg gtcgcaacga
    10381 tgtcggccgc gtcgcgcagg tcggctccgt gcacgtgcaa gagcagatgg tgatcgagcg
    10441 ctactcgcac gtcatgcaca tgacgtcgca tgtcgtcggc acgctcgcga ccggcaagtc
    10501 ctggctcgat gtgctgcgcg cggcgttccc agcaggcacg ctgtcgggtg cgccgaagat
    10561 ccgcgcgatg gagatcatcg acgagctcga gccgcaccaa cgcggcatct acggcggcgc
    10621 ggtgggctat gtcagctaca cgggcaatct cgatctcgcg atcgcgatcc gcacgctcgt
    10681 cagtcacggt gacacgatct acgtgcaagc cggcgctggc ctcgtcgcag actcggttcc
    10741 cgagctcgag taccaagaga ccgtcaacaa agcgcgcgcc gtgctccgcg ctgtcgcaat
    10801 ggcgcgcacc gcgaaggaga gccagcagtg aaggtcctcc tcatcgataa ctacgactcg
    10861 ttcacgtaca acctcgccca atatctcggc gagctaggcg cggacgtctc cgtcgagcgc
    10921 aacgacgcca tctcgctcga tcaaatccgc gagcgcaagc cggccgcgat cgtgatctcg
    10981 ccaggcccgt gcacgccgaa cgaagccggc atctcgatgg acgtcatccg cacgttcgcc
    11041 ggtttgacgc cgatcctcgg cgtgtgcctc ggtcatcaat cgatcggtca ggtgttcggc
    11101 ggtcgcgtag tgcgcgcgcc gcggatcatg cacggcaaga cctcgaagat ctttcacgac
    11161 gaaaagggct tgttctccgg catcgagaat cccttcgtcg cgacgcgcta tcactcgctc
    11221 gtcatcgcac ccgaaacgct tccggactcg ctcgagatca ccgcgaagac gtgggaggac
    11281 gagatcatgg gcgtgcgcca caagcagcac ctcgtcgagg gtgtgcagtt ccacccggag
    11341 tcgatcatga cgacggccgg taagaagctt cttgcgaact tcctcgcaat ggcgcgcggc
    11401 taaccgttcg ggcaatatgc gcggccacat gaagaagacg ttcacactcg caatcgctct
    11461 ctcgctcgcg ctcgtcgccg gctgcggcaa aaaagacgac acggccaaga agacggaagc
    11521 cacgccggcc gacaaggcgc cagccgcgtc gccctcgcca accgcaccgg tcgcgcccgc
    11581 cccgccggac aagctcatgg acgtcgccga gacgcccgcc ggctctccga aagagtgcgc
    11641 cgacgcgcgc gcgatctacg tcaagatcca cgactgcgcg aagctcgacg agaagatgcg
    11701 cgcacagctc gtgaagacgt ggaacgacat ggccgagcaa accctgaagc gctggggcga
    11761 tgcgaacgag cacgaccgcg cgttcatcat cgacacgtgc aagaagctgc cggagaccgc
    11821 cggcatgctc gtgcaggact gctagagacg agcgcatgac ggtcgcgatc gtccgtggaa
    11881 tccagcgcgc tctcgagagg caagacctct cgcgcgccga gatggcggag atcgtcggcc
    11941 agatcatgga cgggcaggcg accgccgcac aaatcggtgg cctgctcgtc gccctgcgcg
    12001 cgaagggcga ggccgtcgac gagctcgtcg gtgccgccgc tgcgatgcgc tcgcgcgcgc
    12061 tcgcgctgca atgcccatcg ctcgatcgca gcatcgacac gtgcggcacc ggcggcgacg
    12121 gctctcacac cgtcaacatc tcgacgttgg ccgcaatcct gatcgcagcc tgcggtggcg
    12181 ttgtcgccaa acacggcaac cgcgccctct catcgaaatg cggctcggcc gacgttctcg
    12241 aagcgctcgg cgtaggcgcc gatgtcgagc ccgccgtcgt agagcgttcg atgcaagtcg
    12301 cgaacatcgg cttcgcgttc gcgccgcgtt ttcacgcagc cacgcgccac gcgtcgggcc
    12361 cgcgcaagga actcggcacg cgcacgatct tcaatctgct cggcccgctg acgaaccccg
    12421 ccaacgtccg ccaccaagtc gtcggcgtgt tcgatcgcaa gtggtgcgag cacgtcgcct
    12481 cggctctcgg cgctctcggc ctgcgccgcg cggccgtcgt gcacggtgaa ggcaacatcg
    12541 acgagatcgc ggtccgcggc gaaacccacg tcgcgatctg ggacgagccc gcctccgtcg
    12601 tgcgcacgct ccaactctcg ccgcggtcgt tcggcgtccg cgaatgcgac ccgtccggcc
    12661 tcgcgggtgg cgacgccgcg ttcaacgcaa acgtcctccg caaagtgatc gccggtcgcg
    12721 actgcgccga cgaccagcgc tacgaagccg tcctccacgc tgccgcgatg acagccgccc
    12781 tcggcctcga gctactgcag gagtcgacgc tcgatctctc gcgcctcccc gtgcactacg
    12841 aacgcgccgt ctccgtcgtg cgctccggcg aagcgcgcct cgtcctccac aagtggcgcg
    12901 aagtctcgaa cgcaaccctc ggcgagtcgc tcgacaacct cgctgcactg aagccgtccg
    12961 actacatcgg tgtcgaatga cctcgggtcg cgccgacatt ctcgagaaga tcctcgccgt
    13021 caaacgcgac gagatcgcag cgctcaaggt gatggcgctc gcggacgcgc cggcacgcgg
    13081 gcaccgttca ttacgagctg cgttgcatcg gagccctgac cagcccatgc gggtgttggc
    13141 cgaagtgaaa cgcgcatcgc cctcggcagg tccgatccgc gcggacgccg atccagtcgc
    13201 aatcgcgcgc gagtacgcgg acgccggcgc cgccgcgatc agcgtgctca ccgaccagca
    13261 attcttcgac ggcgatatcg cgtttctcca gccgtgccac gacgcggtcg acgtcccgat
    13321 cctccgcaaa gacttcttga tcgacccgct gcaagtgcaa gaagccgcgg cccacggtgc
    13381 cgacgcagtg ctcctgatcg tcgccgctct ctcgagctcc gagctcgaag agatgatggc
    13441 cgccgtacac gagcacaagc tcgaggtact cgtcgaggtc cacgacatgg acgagctcga
    13501 caccgcgctc tcgctgtcgc ggccgccgac cgtgatcggc gtgaaccacc gcgacctccg
    13561 cacgttcacg atcgacatga cgctgaccga gcaagtcgcc aagcgcgtgc catcgaacat
    13621 cgtgctcgtt gccgagagtg gtatccgcac cgccgacgac gtccgccgca tgcgcgacgc
    13681 gggcgctcac gccgtcctcg tcggcgagca cctcatgcgc gcgccttcgc cgggtgcagc
    13741 gctgcgcgag ctattggcct gagccgccgc tgctgctcgt tgcgcgacgc aaccgccttc
    13801 gcgcgtagta cgctgtccgg acgatgacgc gcatcaaggt ctgtggcgtg acgctgccgg
    13861 acgacgcagc gcgtgtcgcc gccgcaggcg cagatttcat cggcttcaac ttctggccga
    13921 agtcgaaacg ctacgtcgca cccgagcgcg cacccatgct cgcggccgtc gctcgcgggt
    13981 cgggcgccgc gaagctcgtc ggcgtattcg tcgaccccga catcgacgaa gtcctcgcga
    14041 tcacgacgcg tgtcgacatc gacatcatcc agctccacgg cgacgagaca ccagacttcg
    14101 tcaaacgcct ctcgctctcc gtctaccgcc cggtgtggaa ggcgattccg gtcgcgtctt
    14161 cgaaggacat ccagcatctc gacatctggt catccgaagc gattcttctc gatgccccca
    14221 cgccgggacg cggcggcgcc ggcgcgaagt tcgactggaa cctcgctcgc gaagcgcgcg
    14281 aacgctttcc acgcatcaac atcatcctcg cgggcggcct caatcccgaa aacgtcacga
    14341 cggcgatcca aaccgtcgag ccgtgggccg tcgatgtcgc gagcggaatc gaggcggggc
    14401 ccggcatcaa agatccggcg aagctcgagg cattcgtcgg cgccgcgcgc ctcatcggtt
    14461 agctcgtcga cgcacgcggc ctcgcacctc ggcgtggtat tcaagtgccg atgtcgactg
    14521 ccgacgagat cgcccggtta cccgagcccg tcaacggccg cttcggtccg ttcggcggac
    14581 agtacgtcgc cgagacgctc atgcccgcga tcgcagagct cgagcacgcg tggcgcacgg
    14641 ctcgcaccga cgcgacgttc tggacggaag tcgacgcact cctcgcagac tacgtcggcc
    14701 ggccctcgcg cctctatcac gcacagcgcc tctccgccgc cgtcggcgcg caggtctatc
    14761 tcaagcgcga agacctcaac cacaccggcg cgcacaagat caacaactgc atcggccaag
    14821 cggtcctcgc gcgccgcatg ggcaagcgcc gcctgatcgc cgaaaccggc gcgggccagc
    14881 acggcgtcgc caccgcgacg gtcgccgcgc tgttcgggct cccatgcgag atctacatgg
    14941 gcgccgaaga cgtcgtccgc cagtcgctca acgtcgagcg catgaagctg ctcggcgccc
    15001 aagtccacgc cgtctcgagc ggcacgcgca cgctcaaaga cgcgatgaac gaagcgctgc
    15061 gcgactgggt cacgaacgtc ggcgacactt tctatctcat cggctcggtc gcgggcccgc
    15121 acccctatcc atggctcgtg cgcgacctgc aatccgtcat cggtcgcgaa gcgcacgcgc
    15181 aaatcctcgc gcaagcaggt cgccttcccg acgcgctgat cgcgtgcgtc ggcggtggct
    15241 cgaacgccgc aggcctcttc gcgccgttcg tcgccacccc aggcatccgc ctgttcggca
    15301 tcgaagcggc aggcgagggc gtcgcctccg gccgtcacgc cgcaaccctc agcgccggcc
    15361 gcgtcggcgt gctccacggc agcaagagct acgtcctcac gcacaccgac gatgcgaagc
    15421 tcggccaaat cgcacacgcg cactcgatca gtgccggcct cgactaccca ggcgtcggcc
    15481 ccgagcacgc gcactggaaa gaatccggcg ccgtcacgta cctctcgcgg accgacgacg
    15541 acgccctcgc tgcgctcgag cgcctcgcgc gcaccgaagg catcctctgc gcgctcgaaa
    15601 ccgcccacgc cgtggccgcg atcgacgagg tcgcaaagac gatcggcaaa cccgacccca
    15661 tcatcatcgt cggcctctcc ggccgcggcg acaaggatat ggtcacgatc gcgcagcgtc
    15721 gccacgcagg aggtgcagcg tgacgcatcc gatcgagacg tcgatgacag tgtggcgtcg
    15781 cggaggcgcg tcgtgaaggc ggtgcgcacg gcgctggccg ccgccgcagc agctgctgcg
    15841 atcttcgcgc tactcgcacg ccgaggatcg cgatgaccca cgtgggtgcc gcgcgcgatc
    15901 acgtcgcgat gacccacgtg ggtgccgcac gcgatcacgt cgtgacgagc cacacgcgcg
    15961 tcgagctcgg gtccgccgcg ataacccacg tgggtttcgc accggttcca ggcgcgatgc
    16021 ccccgagcga tgtgcgaggt gcccgatgac ccgcctgcgc gccgcattcg atcgcgccgc
    16081 cgccgagaag cgcgccgcgc tcgtcgcgta catgacgttc gccgatccag atccgagcac
    16141 ctcggtcgcc gttgtcgagg cggcgtgccg cgccggcgcc gacgtcatcg agctgggcgt
    16201 gcccttcagc gatccatccg ccgacggtcc gtcgattcaa cgcgcgatgg agcgcgccct
    16261 cgatgcgggc ggcagcttgc ccggcgcgat cgcggcggtc gccgagctgc gcaagcgcgg
    16321 catcacaacg cccatcgtgc tgttcggcta ctacaacccg atcttcgtga tgggcccggc
    16381 gctgttcgca gagcgcgccg ccaaggccgg cgtcgacgcg gttctcaccg tcgatcttcc
    16441 aatcgacgag ctcgcggagc tcgcgcaacc gctggccgcg cacggcgtac aagtcattcc
    16501 gctcgtcgcg ccgacgtcga cgcccgaacg catcgcgcgc ctcggagccg tgaacgcgcc
    16561 gttcgtctat tacatctcgc tgaccggcgt taccggcggt cgcgcggcag cacccgtcga
    16621 tcccgcgcgc ctcgcgttga tccgcacggc cgcctccgcg ccggtcgcgg tcggcttcgg
    16681 catccgtacg cccgccgacg ctgcgcggtt cgccgcgatc gccgacggcg ttgtcgtcgg
    16741 taccgcgatc gtcgaccaag tcgcggcggg cgatgccgcc ggtgcgcccg atcgcgtcgc
    16801 gaagctcgtc ggcgatctcg ctcgcgcgat gccacgctga gcccaccgcg ccaacgactg
    16861 ctccacgcgg agagcggggg cggacgccac cgagtcggcg gcacgaacga ctggtccgaa
    16921 ccgga
//
LOCUS       DATLBZ010000051        15975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311 k141_40137,
            whole genome shotgun sequence.
ACCESSION   DATLBZ010000051 DATLBZ010000000
VERSION     DATLBZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15975
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40137"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1162)
                     /locus_tag="VMZ53_02580"
     CDS             complement(143..1162)
                     /locus_tag="VMZ53_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27357.1"
                     /translation="MTDSLRAEVVPPRRKSTWLLLVGLTAPLAALSMCERTEKMTVFT
                     PPTLPKVKAVVQPAPEPARVISSELKLVFRAGDHSYVKLSSTEALPRGKAKIVKDERA
                     VHALWAADASALPSWVGTKVFVDNDCTATITRLHYVSRMSIENLEYTEPGRTVSDAMA
                     ERGVFIAGELDGCDGTYARSAALPKVVVPETRPDDAELVKKAKRELLRSPAAKETERV
                     WVEDYQSTETWTSQAQYDIRTFRHPLTAQTWISIHAWNGSGCGDPSVNIWGLFRVDAD
                     GTLFTAQIRDLGDMAKIHQIIDIEGDGGFELIGEPWMPLDTVVTDSHGDVLETLELPY
                     DGCPC"
     gene            complement(1206..2426)
                     /locus_tag="VMZ53_02585"
     CDS             complement(1206..2426)
                     /locus_tag="VMZ53_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HUS27358.1"
                     /translation="MRSLVAIAALLAACEEPSALAPDAAVTLPDAPGGDADVRPRELR
                     GVWITRFAYNSRASLEAIIDRAAGAHFNAVFVQIRGEGDAYYRSAVEPWSARLSGVLG
                     RDPGWDPLQVAIDRAHMHGMELHAYFNVFTAWSASTTIPTAEGSVQHALIAHPEWLAV
                     DSTNTNRDTEYRWFAQGNPAVHAHIVAVAQDLLSHYDVDGLHLDRIRTPGPDYSRDAA
                     TTAAYNAAKAANASLAWADFMRGQVSTMVAEIHDVLVATRPHAKLSASVWGIYQPLPS
                     CSTSQGYGQFYQDSRGWLASGTMDALAPMIYWPIEPGACTDWATLLDGFVAADAGRQI
                     WAGMEAVDDPTWDFTKVQGRIERSRVAGAAGTMVFSSTALDGGAGRWDAFVGTSEVPG
                     PYAEVALPPAMAWK"
     gene            2524..3021
                     /locus_tag="VMZ53_02590"
     CDS             2524..3021
                     /locus_tag="VMZ53_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27359.1"
                     /translation="MRTRLGAAGLWGIAGVIAMLVEAIARLIPVAAAAFDHHFTALHW
                     ILLVVWVLFIGYSEGYRAFHMAFSPRCVVRAQYLSAHPRALHVVLAPLYCMGLIHATK
                     KRLIVSWCVILGITALVILVRQLDQPWRGIIDGGVVIGLSVGVLSILYYAFGKPSPVP
                     PDVPD"
     gene            complement(3018..3398)
                     /locus_tag="VMZ53_02595"
     CDS             complement(3018..3398)
                     /locus_tag="VMZ53_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27360.1"
                     /translation="MRWLLLCWLAGCGRIGFESTSAEVTGETSQAIAVFECTTLFCGD
                     VVCPAGRPCLVSCGPGSCTSIDCSAATECTLSCIGANSCSGDILCGGASCALSCLGAG
                     SCGGSIACDADDCAITCGVLASCR"
     gene            3477..3983
                     /locus_tag="VMZ53_02600"
     CDS             3477..3983
                     /locus_tag="VMZ53_02600"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HUS27361.1"
                     /translation="MSHHVYILEIKKLLSGVEGWLDKTVAYAATKKFDANTLLQARLA
                     PDMFPLVRQIQSTCDSAKYAASRPAGKEPPSHPDTETTIDELKKRVQAVVSYLDTFKE
                     SDFDGADDRRISLPRWEGKSLSGSEYLVHHAQPNFFFHLTTTYAILRHNGVDVGKRDF
                     LGTMPYRQ"
     gene            complement(3984..4226)
                     /locus_tag="VMZ53_02605"
     CDS             complement(3984..4226)
                     /locus_tag="VMZ53_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27362.1"
                     /translation="MRNSIKVAALGVLGAGLVGGAIAGVRYFRARRAEQPLDLETLDI
                     DAIDFGEPVIVAEEIVVVTEPFEADLEAVPAGGFQK"
     gene            4328..5380
                     /locus_tag="VMZ53_02610"
     CDS             4328..5380
                     /locus_tag="VMZ53_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HUS27363.1"
                     /translation="MFAKQTLLSASLVIWLFGATPALAEESTPEAPETGEDHQTGRFL
                     IGAGFSSVEGFIATAQVAQDDLFGTGTQLSLSTRISALRQSFDLRVGHDHLFGSDWGI
                     ATDVYSDSRVMPGFIRDAAGFKTDLSHPLGKHARAFIGYRLEQVTATDTLATSPRALD
                     TYLPPLGGGLNSAVRAGLEWSNVDRLQTRGARIGGTVEVSDPMLGSSYDFSRVHAYAE
                     GHIPLGGLVLHLSGGVESILGAAPRSERIYLGMNDVRGYDQFAIGPVNGLGQPVGGDL
                     ELRGRAELELPLSRRYGISLVGFYDAAAITADRRGQAGASVGFGILWRSPIGNLRFDW
                     AFPIDGDGKPHFVFGL"
     gene            complement(5377..5868)
                     /locus_tag="VMZ53_02615"
     CDS             complement(5377..5868)
                     /locus_tag="VMZ53_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27364.1"
                     /translation="MTTRTVLAALLLVAIPHTSFAGEPTDDECPEAFRGASISAGDLR
                     DGVTLTFTTEHTRILQLRSELRSVATALEQKGTEMQTSSYEEDVEFPPVDLEVFDVDG
                     GARVSVRASRPRDIPELRVLAKSFEKYWSTSTCHGDFIVTPEVPAKKPAAKARRSTRQ
                     ARR"
     gene            complement(5886..6104)
                     /locus_tag="VMZ53_02620"
     CDS             complement(5886..6104)
                     /locus_tag="VMZ53_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HUS27365.1"
                     /translation="MRTRLTDKVSELKRGRGDGHEIARLVEALDRIEKGQYELCAECG
                     EQIGEERLLESPTVTLCVTCASADSWRP"
     gene            complement(6168..6551)
                     /locus_tag="VMZ53_02625"
     CDS             complement(6168..6551)
                     /locus_tag="VMZ53_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27366.1"
                     /translation="MTAYNSGVAGFFDFDWRRLFRWCCLLSIVMAIWLLTPTVKCSYK
                     AFRDTPIGDVESNDVADADKDRVKQGTGFWSQWGTAIKGCYREKPLLTQEPWKRNLLF
                     VFLAVGALAWSLDRIEKRRKRTFDR"
     gene            6660..7853
                     /locus_tag="VMZ53_02630"
     CDS             6660..7853
                     /locus_tag="VMZ53_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27367.1"
                     /translation="MNQRTLVCLLMSSLLACGDDGGGHVHGIPDAKPADAQVCQAVTG
                     SALDYFGYRAASGENGAAIFWTGELGTIDGETLYYDLEFWDGIDTLTGTIDLGAGNQT
                     AYDTCAVCILGYTLDDNGDIARAFFQSGGTMNLAADPVATKNLNATVSGLTMVEISTA
                     DSTPIAGGPCLNYADATLTHDAVPNAWTCDHGKYNSGGNCTCKCGIVDPDCTDASTAA
                     TVEGCNGTTENACFRTADATNTQCVVRPANDTCETAGAALTIGTPVTGTTAGAKHDYD
                     KNLDEATCTNAVAYGYSVPGPDVVYQVTLTQGVPYTITLNGLGSKIDMGVAVVGPAPG
                     TNPASICKTGAPPITNCVAGKDDGGNGIAETFQYTPSATGLYYIIIDSWNYNVGGMFT
                     LNVSQ"
     gene            8082..9530
                     /locus_tag="VMZ53_02635"
     CDS             8082..9530
                     /locus_tag="VMZ53_02635"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HUS27368.1"
                     /translation="MHGSIRAAFLIVIIGACGPTVGNHNGGDDDGTDGGVASCSPGAT
                     QACYTGAPGTQGVGPCHGGTQTCDSSGNWGLCENEVAPTGEVCGNGADDNCNGTADED
                     ADADGDGFSTCAGDCCDATTDGCGDPKLVNPGAFEAPGNTVDDDCDGMVDNVAAAACD
                     MSLPSNSGNAMDFAKAIDLCQTASATDQKWGVISARFTLPSGSGNPNGNQRAIRNVFG
                     GTTVKGGSSFAVLSTGNAAAMGQANPNYAAFQQGNSVGSTSAMPADWLAAHNNTLPNA
                     PNCPAPNGGTTAHDPIMLELKIRTPSNAKSFSLSTNFFSAEFPEYVCSAYNDFFVVLL
                     DSQWAGSPANPADKNLATYTSPTNQIYPVGVNLAFGNTGLFKQCKNGPTGCASGSVAG
                     NITTCANTTELANTGFDIANPSGLPGYCGASNLSGGGTGWLVTSGNVKGGEIITLRIA
                     VWDTSDGILDSLSIIDNFKWSVDASTPGTVIF"
     gene            complement(9538..9972)
                     /locus_tag="VMZ53_02640"
     CDS             complement(9538..9972)
                     /locus_tag="VMZ53_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27369.1"
                     /translation="MTFDAQLRTTLQSIHAKPTPGEARAIIDVARLAAAADKKTDLSE
                     TVVLLALSKIVCDMAGVTEIPDPTASIDPKRLMEIGEQLVPQGARELAYAGAFFVMIS
                     DLELTKEESQLAAGLADALVLDAGRAKQLASQMEALARSARK"
     gene            complement(9989..11764)
                     /locus_tag="VMZ53_02645"
     CDS             complement(9989..11764)
                     /locus_tag="VMZ53_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27370.1"
                     /translation="MRHAWVCVAFAACGSPVAQHAADRPPPLAPAKHIPPPETFTSGS
                     RIATTIDGAYAADEDSGSVVALDGRVLSIGRGLGQLVYDPIADRLYVADRHGDRIVVV
                     DPKSFTIVASYRTPAEPFGIALTPDRSTLLATTIADRTLVAFAVKTGRETWRTRIAPG
                     TRGIAVAPDGERAVLSSVATGELQIVELTGEHRVAEVPFNLECASCGGGGAFPRGTGA
                     VRFLDNTHVVATFQRSIPEALIDLRTDTYGGGSNPPITQHVAFFTFSATSANETVAQL
                     ADNQPRSLAFDPKRDALYIAGHGSDTMIELPKITGASAEAIEGSATNAVLRTNGRCGP
                     DGMAIAGDGSVFVWCAFTRSVMRLSATDELGAAELTQSQQLVATAFTPEQQRGHILFD
                     GTTAMVNRDRALACVTCHVDGQADGLSWRIGKQSLQTPVLAGRIAADTMPYRWDGSAP
                     TLPDSLASTVHRLAGSGLPPAETAAMVAYLRSLPKPRTPTVTDAKAVARGKAIFENTA
                     GCATCHSGPSFTDGELHEFQSSLPSVDTPSLIGLAASAPYYHDGSAPDLYQLLHGRGT
                     VGMSAELAKLSETQLADLQAFLETL"
     gene            complement(11806..13440)
                     /locus_tag="VMZ53_02650"
     CDS             complement(11806..13440)
                     /locus_tag="VMZ53_02650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27371.1"
                     /translation="MSEQSETASASSADLEPGTNVGGYLVDAKIGEGGMGVVYGAHHP
                     RIGKRVAIKVLSRAYCGDPAVVERFEQEARFVNEIKHPNIVDVFQFGELADKRSFFVM
                     EWLEGEPLSALIDRGPVSAAETMEILDVVCDALQAAHEAKVVHRDLKSDNIFLVMKRG
                     KRIVKLLDFGLAKLAGRGVDGQPVGTTKSGILVGTPAYMSPEQARGKAVDGRTDVYAL
                     GCLAYKMLTGTLPFPGDNAMDIILQQLNAPTPNPLKLAPKTPPALAKLVMRMMAKSAD
                     DRPTLVEIRETFAELATTSSTSSSSAPTRKKPISTTFEDTRPVQDRGRKAKPVVAKDD
                     EAERPAPRKRATGVLVAIMLLLAGVIGVAVFMLVNKKDTAAPPAAKTDDVTMTVSAEP
                     IDTGSAATGSAARPTEQPVIEFEEDPVGKGSGAGSAHKPHRKATGSAAGSAEETDVAD
                     EPALPPPNKPGGIIVVLEHASTISIDGKVVGQGTQGGRYEVAPGHHDVKIEASDRSPI
                     TRSVEMDPGGTAFIKIVDDKNSAPEAPKPEAAGSAQ"
     gene            13598..15544
                     /locus_tag="VMZ53_02655"
     CDS             13598..15544
                     /locus_tag="VMZ53_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27372.1"
                     /translation="MRFAYANEDEFVKTYCYYVEQDALFAPTEKVIPPNSECAFSLEL
                     TDGSPMLRGLGVVLDCWTTDDNRFGRRGVYIGFSQLTTTSEAIHARMAALRRAEEEES
                     TEVTQVPVGPPGSSPRTRNAPSTQQQWGPPPPNMRGRSASMAAAEIEETDAGEMRKSI
                     TREMQAASASISTQLAAAVPQPIEERAHNTTARYEGPIRGPAIPAKRAQSPAAALAST
                     LPLPAATSSPATSSSMATSSAGGGRAVSESIPDRGSAPALPPIPVPAPRAIASNPIPP
                     VHIARGSSPAPLHGPAGNAPEQPGASRVAPPVVPANAMASESDISTEPIVVQGSWQAR
                     VDQIRERAHAASKNAWRRAQTTASSGWQTVRAEATQWRADPRTAAQRWKPTRRGVVIF
                     ASGILFGLVLALVVRSSPPAPRAQLAHTSSSSSTHSSKPSTMAALFQQPPAAQCKDSK
                     AETQVALVEAPAASGPAATKKSNVGKSNGAKATGAKPASAKPQAIIATTKPSTAIAKT
                     TQPATTATTKPSTAIAKTTQPATTTATKPSTAVAKTTQPATTATTKPSTGIAKTTQPA
                     TTATTKPSTGIAKTTGIAKTTQPTTSAATKPTSVAKTTQPASTATTTKAPTNKPQVQA
                     KSTAPTKATVAKKPAAKSCGLDCI"
     gene            complement(15645..>15975)
                     /locus_tag="VMZ53_02660"
     CDS             complement(15645..>15975)
                     /locus_tag="VMZ53_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27373.1"
                     /translation="LSWYGYAPTCTAGGASWTTLPMATAADANNQPHIVYAGPPWNNS
                     TTIEDHYRDASGWHVRSFALAKATPLDMKIDAQGTAHILATTPSSTAGSTRLVYLRIN
                     ATGWTAL"
BASE COUNT         2750 a         5332 c         5244 g         2649 t
ORIGIN      
        1 ggcgcatggt gtggccgctc tacgaggcga tgcacgccaa gagcgactgg tggcgaaacc
       61 tagcaaagca gacgttcgag cgcgcgaagg ctgtgtatca cccgatcacg cgcgatgccg
      121 tcgcagagct cctcgcgaag ccctagcagg gacagccgtc gtacggcagc tcgagcgtct
      181 cgagcacgtc gccatgcgag tccgtcacga cggtatcgag cggcatccac ggctcaccga
      241 tgagctcgaa gccgccgtcg ccctcgatgt cgatgatctg gtggatcttc gccatgtcgc
      301 cgaggtcgcg aatctgcgcg gtgaacagcg tgccgtcggc atcgacgcga aacaggcccc
      361 agatgttcac gctggggtca ccgcaaccac tgccgttcca cgcatgaatc gagatccacg
      421 tctgcgcggt tagcgggtgg cggaacgtgc ggatgtcgta ctgcgcctgc gacgtccacg
      481 tttccgtcga ctgatagtcc tcgacccaca ctcgttcggt ttccttcgct gcgggcgagc
      541 gcaatagctc gcgcttcgcc ttcttcacga gctcggcatc gtcgggacgc gtctcgggaa
      601 caacaacctt cggcagcgct gcgctgcgcg cataggttcc atcgcagccg tcgagctcgc
      661 cggcgatgaa cacgccgcgc tccgccatcg catcgctgac cgtgcggccg ggctcggtgt
      721 actcgaggtt ctcgatgctc atgcgcgaga cgtagtgaag cctcgtgatc gtcgcggtgc
      781 aatcgttgtc gacgaacacc ttcgtgccga cccacgacgg aagggcgctc gcgtctgccg
      841 cccagagcgc gtgcacggct cgctcgtcct tcacgatctt cgccttgcct cgcggtaggg
      901 cctcggtcga cgagagcttc acgtacgagt gatcacctgc gcggaacacg agcttcagct
      961 cgctcgagat gacgcgcgcg ggctcgggcg ccggctgcac gacggccttc accttcggaa
     1021 gcgttggcgg cgtgaagacc gtcatcttct cggttcgctc gcacatcgac agcgccgcga
     1081 gaggcgcggt aagcccgacg agcagcagcc atgtgctctt gcggcgagga ggaacgactt
     1141 ctgcgcgaag ggagtccgtc atgctggaga tagaacgcgc gacgagcgcc cgttcatccc
     1201 cgcgatcact tccacgccat cgccggtggc agcgcaactt ccgcatacgg tccaggcact
     1261 tcggaggtgc ctacgaatgc gtcccaccgg ccggctccac cgtcgagcgc cgtcgacgag
     1321 aacaccatcg taccggcggc acccgcgacg cgcgaacgct cgatgcggcc ttgcaccttc
     1381 gtgaagtccc acgtcgggtc atctaccgct tccatgccgg cccaaatctg gcgcccagca
     1441 tcggccgcga caaagccatc gagcagcgtc gcccaatcgg tgcatgcgcc tggctcgatc
     1501 ggccaataga tcatcggcgc gagcgcgtcc atcgtgcccg aggccagcca tccgcgcgag
     1561 tcctgataga actgcccata gccctggctc gtagagcagc tcggtagcgg ctgatagatg
     1621 ccccacaccg atgcggacag cttcgcgtgc ggacgcgtcg ccacgagcac gtcgtggatc
     1681 tccgcgacca tcgtgctgac ttggccgcgc atgaagtcgg cccacgcaag cgatgcgttc
     1741 gcggccttcg ccgcgttgta tgcagcggtc gttgctgcat cgcgcgagta gtcgggcccc
     1801 ggcgtgcgaa tgcgatcgag gtggaggccg tcgacgtcgt agtgcgacag caaatcctgc
     1861 gcgaccgcga cgatatgcgc gtgcacggcc ggattgccct gcgcgaacca gcggtactcg
     1921 gtgtcgcgat tcgtgttcgt cgagtcgacg gcaagccact cgggatgcgc gatcagcgcg
     1981 tgctgcacgg aaccctcggc cgtcggaatc gtcgtcgacg cactccacgc cgtgaacacg
     2041 ttgaagtacg cgtgcagctc catgccgtgc atgtgcgcgc gatcgatcgc gacttgcagc
     2101 ggatcccagc ccggatcgcg gccgagcacg ccggacagcc gcgcagacca cggctcgaca
     2161 gccgagcggt agtacgcatc gccttcgccg cggatctgca cgaacaccgc attgaagtgc
     2221 gcgcccgccg cgcgatcgat gatcgcttcg agcgaggccc tgctgttgta cgcaaaccgc
     2281 gtgatccaca cgccccgcag ctcgcgcggc cggacatcgg catcgccacc cggcgcatcg
     2341 ggcagcgtca ctgcagcgtc cggcgcgagc gcgctcggct cctcgcatgc ggcgagcaac
     2401 gcggcgatag cgacgagcga cctcacgcgc tgaccatagc gatttcatcg cgcgatgcga
     2461 tcgcgattgt cgcctttcag actgcaacat cgaggttgca cactccgcta agctctgcgc
     2521 gacatgcgca cgcgcctcgg agccgccggt ttgtggggca tcgctggcgt gatcgccatg
     2581 ctggtcgagg ccattgcgcg gctcatcccc gtcgctgcgg cggcgttcga tcatcacttc
     2641 acggcgctgc actggatcct tctcgtcgtg tgggtgctgt tcatcggcta cagcgaaggc
     2701 tatcgcgcgt ttcacatggc cttctctccg cgctgcgtcg tccgcgcgca gtacttgtcg
     2761 gcgcatccgc gcgcgttgca tgtggtgctc gcgccgctct actgcatggg cctcatccac
     2821 gcgacgaaga agcgcctgat cgtctcgtgg tgcgtcatcc tcggcatcac cgcgctcgtg
     2881 atcctcgtgc gccagctcga tcagccgtgg cgaggcatca tcgatggcgg cgtcgtgatc
     2941 ggcctgtctg tcggcgtgct gtcgatcctg tattatgcgt ttggcaagcc ttcaccggtg
     3001 ccgccggacg tccccgacta gcggcaggac gcgagcactc cgcacgtgat cgcgcagtcg
     3061 tcggcgtcgc atgcgatcga gccaccgcac gatccggcgc cgaggcacga cagcgcacac
     3121 gacgcgccgc cgcagaggat gtcgccgctg catgaattgg caccgatgca ggacagcgtg
     3181 cattccgtgg ccgcgctgca atcgatgctc gtgcacgagc cagggccgca gctcacgaga
     3241 cacggccgcc ccgccgggca tacgacatcg ccgcagaaca gtgtcgtgca ctcgaacacg
     3301 gcgatcgcct gcgaggtctc gcccgtcact tcagcagagg tcgactcgaa accgatccgt
     3361 ccacagcccg cgagccaaca gaggagcagc catcgcatgc ctccacgtta gggcaggtcg
     3421 gtggattgcc cgacctcacg ccgacctcag cacgatgggc gtgctacgtc agcgggatgt
     3481 cgcatcacgt ttacatcctc gagatcaaga agctgctctc cggcgtcgag ggctggctcg
     3541 acaagaccgt cgcgtacgca gcgacgaaga agttcgacgc gaacacgctg ctgcaagcgc
     3601 gtctcgcgcc ggacatgttc cctctcgtgc gtcagatcca atcgacgtgt gattcggcga
     3661 agtacgccgc gtctcgcccg gctggcaaag agccgccgtc tcaccccgat accgagacca
     3721 cgatcgacga gctgaagaag cgcgtgcagg cggtcgtctc gtatctcgac accttcaagg
     3781 agagcgactt cgacggcgcg gacgatcgcc gcatctcgtt gcctcgctgg gaaggcaagt
     3841 cgctgagcgg gtcggagtac ctcgtccacc acgcgcagcc gaacttcttc tttcacctga
     3901 cgacgacgta cgcgatcttg cgtcacaacg gcgtcgacgt cggcaagcgc gacttcctcg
     3961 gcacgatgcc gtaccgccag tagttacttc tggaagccac cggccggaac ggcttcgagg
     4021 tccgcttcga acggctcggt gacgacgacg atctcttcgg cgacgatcac gggctcgccg
     4081 aaatcgatgg cgtcgatgtc gagcgtttcg agatcgagcg gctgctcggc gcgacgcgct
     4141 cggaagtagc gaacgcccgc gatcgcgccg ccgaccagac ctgcgccgag aacgccgagc
     4201 gcggctacct tgatgctgtt cctcatggtg tgacgtagag caacggtcgt gccgcgaagc
     4261 cgcgctgcga ttggttctca cgcgctgagg caaccggcgg cgcggactgc cggtcgtagc
     4321 ctgcgaaatg ttcgcgaagc agacgttgct gtctgcctcg ctcgtgatct ggctgttcgg
     4381 tgcgacgccc gcgctcgcgg aggagtcgac gccggaggcg ccggaaacgg gtgaggacca
     4441 tcagacaggt aggttcttga tcggtgctgg gttctcgtcg gtggagggct tcatcgccac
     4501 cgcgcaggtc gcgcaagacg atctgttcgg gacaggcacg cagctgtcgc tgtcgacgcg
     4561 gatctccgcg ctccgtcagt cgttcgatct gcgcgtcggc cacgaccatc tgttcggcag
     4621 cgattgggga atcgcgacgg atgtctattc ggacagccgc gtgatgcctg gcttcattcg
     4681 cgacgcagcc ggattcaaaa cggatctttc gcatccgctc ggcaagcatg cgcgtgcgtt
     4741 catcggctac cgcctcgagc aggtgacggc gaccgatacg ctcgcgacgt caccgcgcgc
     4801 gctcgatacg tatctgccgc cactcggagg tgggctcaat tccgcggtgc gtgcgggact
     4861 cgagtggtcg aacgtcgatc gcctgcagac cagaggcgcg cgcatcggcg gcaccgtcga
     4921 ggtcagcgat ccgatgctcg gctcctcgta cgacttctcg cgcgtgcacg cgtacgccga
     4981 gggccacatc ccgctcggcg gtctcgtgct gcacctcagc ggcggcgtgg agtccatcct
     5041 tggtgcggcg cctcgcagcg aacgcatcta cctcggcatg aacgacgtgc gtggctacga
     5101 tcagttcgcc atcggtcccg tgaatgggct cggtcaaccg gtcggcggcg acctcgagct
     5161 aagagggcgc gcggagctcg agcttcccct atcgcgtcgc tacggcatct cgctcgtcgg
     5221 cttctacgac gctgcggcga tcaccgcgga ccggcgaggg caggcgggcg cctcggttgg
     5281 gttcggcatc ctctggcgct cgccgatcgg caacctgcgc ttcgattggg cattcccgat
     5341 cgacggcgac ggcaagccgc acttcgtgtt cggcctctag cgtcgagctt gccgcgtcga
     5401 gcggcgcgcc ttggcggcgg gcttcttcgc cggcacctcg ggcgtgacga tgaagtcgcc
     5461 gtggcacgtc gacgtcgacc agtacttctc gaaggacttc gcgaggacgc gcagctcggg
     5521 gatgtcacgc ggacgcgatg cccgaacgct gacgcgcgcg ccgccgtcga cgtcgaagac
     5581 ctcgaggtcg acaggcggga actcgacgtc ttcctcgtag ctcgaggtct gcatctcggt
     5641 gcccttctgc tcgagggcgg tcgcgacgct gcggagctcg ctgcgaagct ggaggatccg
     5701 cgtgtgctcc gtcgtgaacg tgagagtcac gccgtcgcgg agatcgcccg ccgagatcga
     5761 cgcgccgcga aatgcctcgg ggcactcgtc gtcggtgggc tcgccggcga acgatgtgtg
     5821 cgggattgcc acgaggagca gtgctgcgag cacagtgcgg gtcgtcatga ggttctccaa
     5881 tcgcgtcacg gtcgccagct gtctgcgctc gcgcacgtca cacacagcgt gacggtgggc
     5941 gactcgagca accgctcttc gccaatctgc tcgccacatt cggcgcagag ctcgtattgg
     6001 cctttctcga tcctgtcgag ggcctcgacg aggcgagcga tctcgtggcc gtcgccacga
     6061 ccgcgcttga gctcgcttac cttgtcggtc aggcgtgttc gcatctgctg ccggtccatg
     6121 ggacctgtcc ctgcatcgcg tgggcctggc gccgctgcga gggactctta gcgatcgaac
     6181 gtgcgcttac gtcgcttctc gatgcgatcg agcgaccacg ccagagcgcc gacggcgaga
     6241 aacacgaaca ggaggttccg cttccacggc tcctgagtaa gcagcggctt ctcgcggtag
     6301 cagcctttga tcgcggtacc ccactgcgac caaaacccgg ttccctgctt cacccgatct
     6361 ttgtccgcat cggccacatc gttggactcg acgtcgccga tcggcgtgtc cctaaacgcc
     6421 ttgtagctgc acttgaccgt cggcgtgagc agccagatcg ccatcacgat cgagagcagg
     6481 cagcaccagc gaaagagcct ccgccaatcg aaatcgaaga acccggccac acccgaattg
     6541 tacgccgtca tgcgcgggcg cctaaccgtg cgacacgact cacaacgccc ttcgaaaatc
     6601 aggcgatccg tgacgaaaga cattgcggat ctacccccgt ttttgacagg ttcggcgcca
     6661 tgaatcagag gactctcgtc tgtttgttga tgtcgtcact gctcgcgtgc ggcgacgacg
     6721 gtggtgggca cgttcacggc atccccgacg cgaagccggc cgatgcgcag gtttgccaag
     6781 cggtgacggg tagcgcgctc gattacttcg gctaccgcgc cgcgtccggc gagaacggcg
     6841 ccgcgatctt ctggactggc gagctcggca cgatcgacgg cgagaccctc tactacgatc
     6901 tcgagttctg ggacggaatc gacacgctca cgggcacgat cgatctcggt gcgggcaacc
     6961 agacggcata cgacacgtgt gcggtctgca tcctcggcta cacgctcgat gacaacggcg
     7021 acatcgctcg cgcgttcttc cagtcgggcg gcacgatgaa cctcgcggcc gatccggttg
     7081 cgacgaagaa cctgaacgcg acggtcagcg gtctcacgat ggtcgagatc agcacggcgg
     7141 actcgacgcc gatcgctggc ggcccctgcc tcaactacgc ggacgcgacg ctcacgcacg
     7201 acgccgtccc gaacgcgtgg acgtgtgacc acggcaagta caacagcggc ggtaactgca
     7261 cctgcaagtg cggcatcgtc gatccggact gcacggacgc gagcacggcc gcgaccgtcg
     7321 aaggttgcaa cggcacgacc gagaacgcgt gcttccgcac ggcggacgcg acgaacacgc
     7381 agtgcgtggt tcgcccggcg aatgacacgt gtgaaacggc aggcgctgcg ctcaccatcg
     7441 gcacgccggt gacgggaacg acggccggcg cgaagcacga ctacgacaag aacctcgacg
     7501 aagcgacctg cacgaacgcc gtcgcatacg gctacagcgt cccgggtccc gacgtcgtct
     7561 atcaagtcac gctgacgcag ggcgtgccgt acacgatcac gctcaacggc ctcggctcga
     7621 agatcgatat gggcgttgcc gtcgtcggtc cggcgccggg tacgaacccc gcgtcgatct
     7681 gcaagacggg cgcgccgccg atcaccaact gcgtcgccgg taaggacgat ggcggcaacg
     7741 gcatcgccga aacgttccag tacaccccga gcgcgacggg tctctactac atcatcatcg
     7801 actcgtggaa ctacaacgtc ggcggcatgt tcacgctgaa cgtctcgcag taagcagaca
     7861 gctcctcagc cagggccacg tcggaaacgg cgtggctttt gtcttttcgg gcgggtagcg
     7921 cgtcctacat ctacgggcgt cggcgcaggc gtctcctctg tgcgctaaca ccctctcgat
     7981 aggcacgtgg gtcgatctgc gcagaagtga tcaacaagcg cagaatggaa attgcgggcc
     8041 gtgtgtgaca cggaggacgc tgcgccgcga gggaggccgc catgcacggt tctattcgcg
     8101 ctgcgttctt gatcgtcatc attggtgcgt gtggtccgac ggtcggtaac cacaacggtg
     8161 gtgacgacga cggaaccgac ggtggtgtcg cgtcgtgctc tcccggcgca acgcaagctt
     8221 gctacaccgg tgcgccgggc acgcaaggcg tcggaccctg ccatggcgga acgcagacgt
     8281 gcgactcgag cggcaattgg ggcctctgcg agaacgaagt cgcgccgacg ggagaagtct
     8341 gcggcaacgg cgccgacgac aactgcaacg gcactgccga tgaagacgcc gacgcggatg
     8401 gcgacggatt ctcgacgtgc gccggcgact gctgcgacgc gacgacggac ggctgcggcg
     8461 atccaaagct cgtgaacccg ggcgcgttcg aagcgccagg caatacggtc gacgatgact
     8521 gcgacggcat ggtcgacaac gtcgcggcgg cggcgtgtga catgagcttg ccgagcaact
     8581 ccggcaacgc gatggacttc gcgaaggcga tcgacctgtg ccagacggca tcagcgacgg
     8641 atcagaagtg gggcgtgatc agcgcgcgct tcacgctgcc gagcggaagc ggcaacccga
     8701 acggcaacca gcgcgcgatc cgcaacgtgt tcggcggcac gaccgtgaaa ggcggcagct
     8761 cgtttgcggt gctgtcgacc ggcaacgccg cagcgatggg ccaagcgaat ccgaactacg
     8821 ccgcgtttca gcaaggcaac tccgtcggct cgacgagcgc gatgcccgct gactggctcg
     8881 ctgcgcacaa caacacgctg ccaaacgcgc cgaactgtcc ggcgccgaac ggcggcacga
     8941 cggcgcacga cccgatcatg ctcgagctga agatccgcac gccgtcgaat gcgaagtcgt
     9001 tcagcctctc gacgaacttc ttctcggccg agttcccgga gtacgtgtgc agcgcgtaca
     9061 acgatttctt cgtcgtgcta ctcgattcgc agtgggcggg ctcgccggca aacccagccg
     9121 acaagaacct cgcgacgtac acctcgccga caaaccagat ctatccggtc ggcgtgaacc
     9181 tcgcgttcgg caacacgggc ctcttcaagc aatgcaagaa cggcccgacg ggctgtgcgt
     9241 ccggctccgt cgccggaaac atcacgacat gcgcgaacac gacggagctc gcgaacacgg
     9301 gcttcgatat cgcgaatccg tcggggcttc ccggctactg cggcgcgagc aacctcagcg
     9361 gcggtggcac gggctggctc gtcacgtccg gcaacgtgaa gggcggcgag atcatcacgc
     9421 tgcgcatcgc ggtgtgggac accagtgacg gcatcctcga ttcgctgtcg atcatcgaca
     9481 acttcaagtg gtcggtcgac gcgtcgacgc ccggtacggt gatcttctag tagcgagcta
     9541 cttgcgagcg gacctggcga gcgcttccat ctgagaagcg agctgcttgg cgcggccagc
     9601 gtcgaggacg agcgcgtcag cgaggcctgc tgcgagctgg ctctcttcct ttgtcagctc
     9661 gaggtcggaa atcatcacga agaacgcgcc ggcgtacgcg agctcgcgcg cgccttgcgg
     9721 aacgagctgc tcgccgatct ccatgagccg cttcggatcg atcgaggcgg tcggatccgg
     9781 aatctcggtg acacctgcca tgtcgcagac gatcttcgag agcgcgagca agacgacggt
     9841 ctcgctgagg tcagtcttct tgtcggccgc ggcggcgagg cgcgcgacgt cgatgatcgc
     9901 gcgtgcttct cccggcgtcg gcttggcgtg aatcgattgg agtgtggtgc ggagctgggc
     9961 gtcgaaggtc acgcttcaac gctaccactc acaaggtctc gagaaacgcc tggaggtcgg
    10021 caagctgcgt ctcgctcagt ttcgcgagct ccgccgacat tccaacggta ccgcggccgt
    10081 gcagcagctg gtagagatcg ggtgcgctgc cgtcgtgata gtacggcgcg cttgcggcga
    10141 gcccgatcag gctcggtgtg tcgacactcg ggagcgagct ttggaattcg tggagctcac
    10201 cgtcggtgaa tgacggcccc gagtgacacg tcgcgcagcc ggctgtgttc tcgaagatgg
    10261 ctttgccgcg cgcgacggcc tttgcatctg tgaccgtggg cgtgcgcggc tttggcagcg
    10321 agcgcaggta cgcgaccatc gcggctgtct cggcgggcgg cagccccgag ccggcgaggc
    10381 gatgcacggt gctcgcgagg ctatctggca gcgtcggcgc ggagccatcc cagcgatacg
    10441 gcatcgtgtc ggcggcgatg cggccggcga gcaccggcgt ctgcagcgat tgcttgccga
    10501 tgcgccacga caggccgtcg gcttggccgt cgacgtggca cgtcacgcac gcgagcgcgc
    10561 gatcgcgatt gaccatggcg gtcgtaccgt cgaacagaat gtggccgcgc tgttgttccg
    10621 gcgtaaatgc ggtcgcgacg agctgctggc tctgcgtgag ctcggcggcg ccgagctcat
    10681 cggtggcaga gagccgcatc acgctgcgcg tgaacgcgca ccagacaaag acgctgccgt
    10741 cgccggcgat tgccatgccg tcgggcccgc aacggccgtt cgtgcgcagc accgcgttcg
    10801 tcgcgctgcc ttcgatggct tcggcgctgg cgcccgtgat cttcggcagc tcgatcatcg
    10861 tgtcggagcc gtggccggcg atgtagagcg catcgcgctt cggatcgaag gcaagcgagc
    10921 gcggctggtt gtcggcgagc tgtgccacgg tctcgttcgc gctcgtcgcg ctgaacgtga
    10981 agaaggcgac gtgttgcgtg atcggtggat tcgagccgcc gccgtacgta tccgttcgca
    11041 gatcgatgag cgcctcgggg atcgagcgct ggaacgtcgc aacgacgtgc gtgttgtcga
    11101 ggaagcggac ggcgcccgtg ccacgtggga atgcgccgcc accgccacaa ctcgcgcact
    11161 cgagattgaa cgggacttcc gcgacgcggt gctcgccggt gagctcgacg atctgcagct
    11221 cgccggtcgc aaccgacgag agcacggcgc gctcgccatc gggtgccacc gcgatgccgc
    11281 gggtgccggg cgcgattcgg gtccgccacg tctcgcgtcc cgtcttcacc gcgaacgcga
    11341 cgagcgtgcg gtcggcaatc gtcgtggcga gcagcgtcga acgatcgggc gtcagtgcga
    11401 tgccgaacgg ctctgcgggt gttcgatagc tggccacgat cgtgaagctc ttcggatcga
    11461 cgacgacgat tcgatcgccg tgccgatctg cgacgtagag ccggtctgcg atcgggtcgt
    11521 agacgagctg gccgagccca cggccgatcg agagcacacg gccgtccaga gcgacgaccg
    11581 agccgctgtc ctcgtcggcg gcatacgcgc cgtcgatcgt cgtcgcgatc cgcgagccgc
    11641 tcgtgaacgt ctcgggtgga gggatatgct tcgcgggcgc gagcggcggc gggcgatccg
    11701 ctgcgtgctg cgcgactggc gaaccacagg ccgcgaacgc aacgcagacc catgcgtgtc
    11761 tcatcgccgc gtgggacaac gacgcgcgag attggttgcc agaaactact gcgccgagcc
    11821 ggcagcttcg ggcttaggtg cttcgggtgc gctgttcttg tcgtcgacaa tcttgatgaa
    11881 ggccgtgccg cccggatcca tctcgacgct gcgcgtaatc ggcgaacgat ccgacgcctc
    11941 gatcttcaca tcgtggtggc cgggcgcgac ctcgtagcga ccgccttgtg ttccctgacc
    12001 gacgaccttc ccgtcgatcg agatcgtcga cgcgtgctcg agcacgacga tgatcccgcc
    12061 gggcttattc ggtggcggga gcgcaggctc gtcggcgacg tcggtctcct ccgccgagcc
    12121 ggcagcggag ccggtcgcct tgcgatgagg cttgtgcgcg cttcccgcgc ccgagccttt
    12181 gccgaccgga tcttcttcga actcgatcac gggctgctcg gtggggcgcg ccgccgaccc
    12241 ggtcgccgca gatccggtat cgatgggctc agcggacacc gtcatcgtga cgtcgtcggt
    12301 cttcgccgcg ggtggcgctg ccgtgtcctt cttgttgacg agcatgaaga ccgcgacacc
    12361 gatgacgccc gcgagcaaga gcatgatcgc cacgagcacg ccggtcgcgc gcttgcgcgg
    12421 agcagggcgc tccgcttcgt cgtccttggc aacaaccggc ttcgccttgc gcccgcgatc
    12481 ctgcacgggg cgggtgtctt cgaatgtcgt cgagattggc ttcttgcgcg tcggagctga
    12541 ggacgacgag gtcgacgatg tcgtcgcgag ctcggcgaac gtctcgcgaa tctcgacgag
    12601 cgtcgggcga tcatcggcgc tcttcgccat catgcgcatg acgagcttcg cgagcgcggg
    12661 cggcgtcttg ggcgcgagct tcagcggatt gggtgtcggc gcgttgagct gttggaggat
    12721 gatgtccatc gcgttgtcgc cggggaacgg caacgtgccg gtgagcatct tgtacgcgag
    12781 gcaaccgagc gcgtagacgt cggtgcggcc gtcgaccgcc ttgccgcgcg cttgctcggg
    12841 cgacatatac gcgggcgtac cgacgaggat tccagacttc gtcgtcccga cgggctgacc
    12901 atcgacgccg cggccggcga gctttgcgag cccgaagtcg agcaacttca cgatgcgctt
    12961 gccgcgcttc atcacgagaa agatgttgtc gctcttcaga tcgcgatgga cgaccttcgc
    13021 ttcgtgcgca gcctgcagtg cgtcgcacac gacgtcgagg atctccatcg tctccgcagc
    13081 ggacaccgga ccgcgatcga tcagcgcgct cagcggctcg ccttcgagcc actccatgac
    13141 gaagaagctg cgcttgtcgg caagctcgcc gaactgaaag acatcgacga tgttcgggtg
    13201 cttgatctcg ttgacgaacc gcgcctcttg ctcgaagcgc tccacgaccg cggggtcgcc
    13261 gcagtacgcg cgcgacagca ccttgatcgc gacgcgctta ccgatgcgcg gatgatgcgc
    13321 gccatagacg acgcccatgc cgccttcgcc gatcttcgcg tcgacgaggt acccgccgac
    13381 gttcgtgccc ggctcgagat ccgcgctcga cgcggaagct gtctccgact gctcggacat
    13441 aggcgattca ggcagcctaa ctgcgccgtg ggcacttcgc caggccgttt tgactgcatg
    13501 tgtgcaaagg ccgcaccctg cctccctacc atgtgcctcc acggcctcgt aggatcgaaa
    13561 ggtggtgggg acgaacactc gtccaagagg gatacgcgtg cgctttgcgt acgcgaatga
    13621 ggacgagttc gtcaaaacgt actgctacta cgtcgagcaa gacgctctct tcgcaccgac
    13681 ggaaaaagtc atcccgccaa acagcgagtg cgcgttctcg ctcgagctga ccgacggaag
    13741 cccgatgctc cgcggcctcg gcgtcgtgct cgactgctgg acgacggacg acaatcggtt
    13801 cggccgccgc ggcgtataca tcggcttctc gcagctgacg accacgagcg aagcgatcca
    13861 cgcgcgcatg gccgcgctgc gcagagccga agaagaagag agcacggagg tcacgcaagt
    13921 cccggtcggc ccgccgggct catcgccgcg cacgcgcaac gcgccgtcga cccagcagca
    13981 gtggggaccg ccgccgccga acatgcgcgg ccgcagcgcg tcgatggccg ctgccgaaat
    14041 cgaagagacc gacgcgggcg agatgcgcaa atcgattacg cgcgagatgc aggccgcgtc
    14101 ggcatcgatc tcgacacagc tcgccgccgc cgtgccacag ccaatcgagg agcgcgcgca
    14161 caacacgacc gcgcgctacg aaggtccgat tcgcggaccg gcaatccccg caaagcgcgc
    14221 gcagtcgccc gcggccgcgc tcgcctccac gctgccgcta ccagccgcga cgtcgtcgcc
    14281 ggccacttcg tcgtcgatgg ccacttcgtc agcaggcggc ggtagagcgg tcagcgaatc
    14341 gattcccgac cgcggctcgg caccggcgct gccgccgatc ccagtcccgg cgccgcgcgc
    14401 gatcgcaagc aatccgattc cgccggtcca catcgcgcgc ggttcgtcgc ccgcgccgct
    14461 acacggcccc gcgggcaacg cgcccgaaca gcctggcgca tcgcgtgtcg ccccgccggt
    14521 cgttccggcg aacgcgatgg cctccgagag cgacatctcg acggagccga tcgtcgtgca
    14581 gggctcgtgg caggctcgcg tcgatcagat tcgcgagcgc gcgcacgctg ccagcaaaaa
    14641 tgcgtggcgt cgcgcgcaga ccaccgcgtc gagtggctgg cagacggtgc gcgccgaagc
    14701 cacgcaatgg cgcgcggatc ccaggaccgc cgcgcagcgc tggaaaccaa cacgccgcgg
    14761 tgtcgtcatc ttcgcaagcg gcatcctgtt cggcctcgtg ctcgcgctcg tcgtccgctc
    14821 atcgccgccc gcgccgcgcg cgcagctcgc acacacgtcg tcgtcgtcgt cgacgcactc
    14881 gtcgaagccg tcgacgatgg ccgcgctgtt ccagcagcca ccggcggccc agtgcaagga
    14941 ctcgaaggcc gaaacgcagg tcgcgcttgt cgaggcgccc gccgccagcg gtcccgcagc
    15001 gacgaagaag tcgaacgtgg gcaagtcgaa tggtgcaaag gcgactggcg cgaagccagc
    15061 aagcgcgaag ccccaggcga tcatcgctac gacgaaaccc tcgacggcca tcgcaaagac
    15121 gacgcagccc gcgacaaccg cgacgacaaa accctcgacg gccatcgcaa agacgacgca
    15181 gcccgcaacg accacggcga cgaaaccctc gacggccgtc gcaaagacga cgcagcctgc
    15241 gacgaccgcg acgacgaaac cctcgacggg catcgcaaag acgacgcaac ctgcgacgac
    15301 cgcgacgacg aaaccctcga cgggcatcgc gaagacgacg ggcatcgcga agaccacgca
    15361 acctacgacg agcgcggcca cgaagccgac ctcggtcgcg aagacgacgc agcctgcgag
    15421 caccgcgacg acgacaaagg ccccgacgaa caagccgcag gttcaggcca agagcacggc
    15481 accgaccaag gcgacggtcg cgaagaaacc cgccgcaaag tcctgcggcc tcgactgcat
    15541 ctagcgcgtt ggcgttcgca gctcgtcgcg gtcgcaacat actgctgcat cgcatcgact
    15601 gcgtctaagc gcgtgaaagc gctgtccagc ccgtcgcgtc ggcgttacag cgcggtccag
    15661 cctgtcgcat tgatgcggag gtagacgagg cgcgtggagc ccgccgtcga gctcggcgta
    15721 gttgcgagaa tgtgcgcggt gccttgcgca tcgatcttca tgtcgagcgg cgtcgctttt
    15781 gcgagcgcga agctgcgcac gtgccagccg gatgcgtcgc ggtagtgatc ctcgatggtc
    15841 gtcgagttgt tccagggcgg gcccgcgtac acgatgtgcg gctggttgtt tgcgtcggcg
    15901 gccgtcgcca tcggcagcgt cgtccacgat gcgccacctg cggtgcacgt tggtgcgtag
    15961 ccgtaccagg agagg
//
LOCUS       DATLBZ010000052         6588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7473864, whole genome shotgun sequence.
ACCESSION   DATLBZ010000052 DATLBZ010000000
VERSION     DATLBZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7473864"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VMZ53_02665"
     CDS             <1..1228
                     /locus_tag="VMZ53_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27374.1"
                     /translation="TNIAGSEVTERLAGLRLRDEMVAVLLSRILGDAYNRWRDRSKSA
                     GVEALPLDLGILGEIDYADHFRDFDPRGLWTFLEHLVAQSMHVYTQVELIDLDTVRLL
                     GLFKEDSASGSEALGGAIDLVDLFSALGSPEANDVANFSLDLLPSVLETRRSTGAQTF
                     AVDGYASIERKGNIDSLVLSELAYDREIFEQKVLDNELLYYGRDKQREDERRLQYLLI
                     DCSASMRGQRQVFARGLALALIKKLTLEGDEIWVRFFDSRLHETIKIGRSGQVPVPYL
                     LSFRSERGRNYAKVFRQLLVEVTRLRREQKRRVVMYVITHGQCHIEPELISTLKQQAF
                     LYGVVCLPSSDQLPEFVPLLDRSQIVTSDALTSRAERQRRALDIVGDATRAPKGPKPQ
                     TTAPSASMRNMPVVKP"
     gene            1232..3043
                     /locus_tag="VMZ53_02670"
     CDS             1232..3043
                     /locus_tag="VMZ53_02670"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS27375.1"
                     /translation="MARRTFNSIESTADQAEAFVQQGRWLEAERHYRELIGQTHVINY
                     EYDDWLRRLGDIYRQLGRQREAAFVYLYLHYFDLARGQLQAEQHVSLRARLAELEKKW
                     SEAAALYAQAKLPVHAAVSYERAKQYPEAAQAWKGMTHMAELGHRAYEAALVHFNYGL
                     AANRLDANSSEARRALIESQRKLEQVADDFEQVGELERAFDCFQILLKLGKESAQFEN
                     LAEGYVNCIRVLRDDNLKFYVLQYYEDFIKLALERGELHAAATLYQEAAAFAARAGLP
                     YDRHYQHKSALTWIKCAERFVETGVPVQMIENALLAAASQHSAVGDYPAVRDCFDRLA
                     GLELPERAKKRFSTIAQRYKGLASPPVELPGLPDYLKQQHAYGDIWFVDLLEWEMDGD
                     PFAVATSIVGDLKYPNGIRRRALVVLLNLADAEQRNAVGETETLVDVAEKLGELQSYA
                     ALSPLERLYASQDAAVRYAAVRALRFLYFKRSFVIVRKALQDADAKVREAALIAIGGL
                     HFPHAFNPLARIYRESTDPRVRAAALQSIGKIQTVEAGEFLVMVLRQEGGDLRTAAVN
                     ALASMDNADVVPILRQHHEIETNPQVRETLGELLRRH"
     gene            3101..3721
                     /locus_tag="VMZ53_02675"
     CDS             3101..3721
                     /locus_tag="VMZ53_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27376.1"
                     /translation="MSPGAVDLPRRRVELQLGKRRHRRITGPSALLLFVCLFLPAVKG
                     CGEPVVPITMPELIHPYLFGAVIAFGASAFTLRGLRNTIRAMRALAWLTIGGACVLAV
                     VVPPLAFIELILGFVLLAAIGRHGYSEKRAAITTFVVGMLSLVWFGLWALSPDALIGV
                     YLSSIGSAGLMLGGLVWLSETAYDSSHGWIQPDGIPRAARVRSRGM"
     gene            3660..4589
                     /locus_tag="VMZ53_02680"
     CDS             3660..4589
                     /locus_tag="VMZ53_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27377.1"
                     /translation="MGGFSRMAFLALLAFAVAGCDSRAKASDPQVRSESKSKEYESCG
                     ASLHCQDDLRCFDNVCRRGARSNVGDYFAALGAQQLARGETEASIDSYNKALGHYDSE
                     KIGLPPDVDCAYGAALAAGKQKKEHAELAARVLHRCILAVPVGSVLRTQALAQLAGIT
                     DAGLDPLSLGRTQLADVYLTRAPQKPSSDKIGVTIAANPPIDKKTYHFIPDRLAEQDM
                     RNALVACWEQYNNATHKDTLVASVGVKVSYVASEYEDEPGSFSTKLEPAVAMPAGPEA
                     TADQCVRAAVEPAIKGLATLRDAFATKLTITIK"
     gene            complement(4609..4983)
                     /locus_tag="VMZ53_02685"
     CDS             complement(4609..4983)
                     /locus_tag="VMZ53_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27378.1"
                     /translation="MRNREVIEREIHDARQDLEASLAELRHVVQEKVDVKARARVAVA
                     KGKLRAEELYEEYGAKAKFFLQKGKFEAQQLANKGKDGARDLYVKGRETVEERPVLIG
                     SIAAGVIAVGAFIYIGRKNEWF"
     gene            complement(4993..5421)
                     /locus_tag="VMZ53_02690"
     CDS             complement(4993..5421)
                     /locus_tag="VMZ53_02690"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HUS27379.1"
                     /translation="MGSYVNPNRPAETSGHGVGELLHRITDDLKTIAKDEIDLAKNEV
                     ARHAKLAAGEAAIIVLGGIVALIGFAMLCTAAVVALEPVIHSLAVRLLLMSLIYLIGG
                     AVVAGVFAKRLGNDIKPDLAVPAYEAKRTIAGVKESLSHS"
     gene            complement(5432..5560)
                     /locus_tag="VMZ53_02695"
     CDS             complement(5432..5560)
                     /locus_tag="VMZ53_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27380.1"
                     /translation="MKKLAVNALLQSVSATAEQLGVRALLRMWQRATTRQLAGKSL"
     gene            5646..>6588
                     /gene="rlmN"
                     /locus_tag="VMZ53_02700"
     CDS             5646..>6588
                     /gene="rlmN"
                     /locus_tag="VMZ53_02700"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HUS27381.1"
                     /translation="MEKPDLCAMTLADAEAWAVAQGWARFRGEQVWRWVHDRNARSFE
                     EMTNLPAEVRTSLAERAQIGSLKIAEIQTSRDGTRKLRLETRDGQSIESVLIPDGEKT
                     TQCISSQVGCAVDCQFCATAKMGLKRNLDVGEIVDQVYLARRLLAEVEPGRKLTNLVY
                     MGMGEPLHNYDNMVKSLRILCNDKGAKLGQRRVTVSTSGLVPKLEKLGNEDVRPNLAV
                     SLNAPNDEIRDQIMPINRKWNIGKLLAALKAYPLEQRRRITFEYVLLANVNDSLRDAG
                     QLAKLLRGFRCKVNIIPWNPHPEAPYERPADAVIEAFQ"
BASE COUNT         1153 a         2133 c         2135 g         1167 t
ORIGIN      
        1 gacgaatatc gcggggtcgg aagtcaccga gcgcctcgct gggttgcggc tccgcgacga
       61 gatggtggcc gtgctgctgt cgcgcattct tggtgacgcg tacaaccgct ggcgcgatcg
      121 gagtaagtcc gccggcgtcg aagcgctgcc cctcgacctc ggcatcctcg gcgagatcga
      181 ctatgccgat cactttcgtg acttcgatcc gcgcgggctg tggacgttcc tcgagcacct
      241 cgtcgcgcag tcgatgcacg tctacacgca ggtcgagctc atcgatctcg atacggtgcg
      301 gttgctcggc ctgttcaaag aggacagcgc gtcgggaagc gaggcgctcg gcggcgcgat
      361 cgatctcgtc gacttgttca gcgcgcttgg ctcgcccgag gcgaacgacg tcgcgaactt
      421 ctcgctcgat ctgctgccga gcgtgctcga gacgcggcgc tcgaccggtg cgcagacgtt
      481 tgcggtggat ggctatgcgt cgatcgagcg caagggcaac atcgattcac tcgtgttgtc
      541 ggagctcgcg tacgaccgcg agatcttcga gcagaaggtg ctcgacaacg agctgctgta
      601 ttacggccgc gacaagcagc gcgaggacga gcggcgcctg cagtacttgc tcatcgattg
      661 ctcggcctcg atgcgcggcc agcgtcaggt gtttgcgcgc ggattggcgc tcgcgctcat
      721 caagaagctc acgctcgagg gtgacgagat ctgggtgcgc ttcttcgact cgcgcttgca
      781 cgagacgatc aagatcgggc gatccgggca ggtgccggtg ccatatctgt tgtcgttccg
      841 cagcgagcgt ggtcgcaact acgcgaaggt gtttcggcag ctgctggtcg aggtcacgcg
      901 gctgcggcgc gagcagaagc ggcgcgtcgt gatgtacgtg atcacgcacg gccaatgtca
      961 catcgagccc gagctcatct cgacgctgaa gcagcaggcg ttcctgtacg gcgtcgtgtg
     1021 tctgccgtcg tcggatcagc tgcccgagtt cgtgccgctc ctcgatcgct cgcagatcgt
     1081 gacgtcggat gcgctgacgt cgcgcgccga acggcagcgg cgagcactcg acatcgtcgg
     1141 tgatgcgacc cgcgcgccga agggaccgaa gccgcagacg accgcgccga gcgcgagcat
     1201 gcgcaacatg ccggtcgtga agccctgacc gatggcacgt cgtacgttca actcgatcga
     1261 gagcacggcg gatcaggccg aggcctttgt acaacagggc aggtggctcg aagcggagcg
     1321 ccattatcgc gagctgatcg gtcagacgca cgtcatcaac tacgagtacg acgattggtt
     1381 gcgccgcctc ggcgacatct atcgtcagct cggccgtcag cgcgaagcgg cgttcgtgta
     1441 cctgtatctg cactacttcg atctcgcgcg cgggcagctg caggccgagc agcacgtttc
     1501 gctgcgtgcg cgcctcgccg agctcgagaa gaagtggagc gaagccgctg cgctctatgc
     1561 gcaggcgaag ctgcccgtgc atgccgcggt gtcctacgag cgcgcgaagc agtatccaga
     1621 agcggcacag gcgtggaagg gcatgacgca catggcggag ctcgggcatc gcgcgtacga
     1681 agcggcgctc gtgcacttca actacgggct cgcggcgaac cgcctcgacg cgaattcgag
     1741 cgaagcgcgc cgcgctctga tcgagagcca gcgcaagctc gagcaagtcg ccgacgactt
     1801 cgagcaggtt ggcgagctcg agcgcgcatt cgactgtttc cagatcctgc tgaagctcgg
     1861 caaggaatcg gcgcagttcg agaacctcgc cgagggttac gtcaactgca tccgcgtgct
     1921 gcgcgatgac aacctcaagt tctacgtcct gcagtactac gaggacttca tcaagctcgc
     1981 gctcgagcgc ggcgagctgc acgcggcagc gacgctctat caagaagcgg cggcctttgc
     2041 ggcacgcgca ggcctaccgt acgaccggca ctaccagcac aagagcgcgc tgacatggat
     2101 caagtgcgcc gagcgcttcg tcgagaccgg cgtccccgtc cagatgatcg agaacgcgct
     2161 gctcgcggcg gcgtcgcagc attcggctgt cggcgactac ccagcggtgc gcgactgctt
     2221 cgatcggctc gcgggcctcg agctgccgga gcgcgccaag aagcggttct cgacgatcgc
     2281 gcagcgctac aaaggcctcg cgtcgccgcc cgtcgagctt cccggcctcc ccgattatct
     2341 caagcagcag cacgcgtacg gcgacatctg gttcgtcgac ctcctcgagt gggagatgga
     2401 tggcgatccc ttcgcggtcg cgacgtccat cgtcggtgac ctcaagtatc cgaacggcat
     2461 ccgccgccgc gcgctcgtcg tgctgctcaa cctcgcagac gccgagcagc gcaatgccgt
     2521 cggcgagacc gagacgctcg tcgatgtcgc ggagaagctc ggagagctgc agtcgtatgc
     2581 ggcgctgtca ccgctcgagc ggctgtatgc gtcgcaggat gcagcggttc gctacgccgc
     2641 cgtgcgcgcg ctgcgcttcc tttacttcaa gcggtcgttc gtcattgtcc gcaaggcgct
     2701 gcaggatgcg gacgcgaaag tccgcgaggc ggcgctgatc gcgatcggcg gtttgcactt
     2761 cccgcacgcg ttcaacccgc tcgcgcgcat ctatcgcgag tcgacggacc cgcgcgtgcg
     2821 tgcagcggcg ctccagtcga tcggtaagat ccagaccgtc gaggcaggcg agttcctcgt
     2881 gatggtcctg cgccaagaag gtggcgattt gcgcactgcg gcagtcaacg cgctcgcgtc
     2941 gatggataac gccgacgtcg tgccgatcct ccgccagcat cacgagatcg agacgaatcc
     3001 gcaggttcgc gagacactcg gtgagttact gagacgccat taattgccgc gtcgaagtgg
     3061 cggtctcgtg tcatcctcgc cggcagtgga acacgggtgt gtgtctccag gggcagtcga
     3121 tctgcctcgt cgacgcgtcg agctccagct cggcaagcgc cggcatcgcc gcatcacagg
     3181 gccatctgct ctgctgctct tcgtgtgcct gtttctcccc gccgtcaaag gctgcggcga
     3241 accggtcgtt ccgatcacga tgcccgagct gattcatccg tatctgttcg gcgcggtgat
     3301 cgcgttcggt gcgtccgcgt tcacgctgcg cggcctgcgc aacaccatcc gcgcaatgcg
     3361 cgctctcgcg tggctgacga tcggcggcgc gtgcgtgctc gcggtcgtcg tgccgccgct
     3421 cgcgttcatc gagctgatcc ttggcttcgt cctgctcgct gcgatcgggc gccacggcta
     3481 ctccgagaag cgcgccgcga tcacgacgtt tgtcgtcggc atgctgtcgc tggtttggtt
     3541 cggactgtgg gcactcagcc ccgacgcgct catcggcgtc tatctctcga gcatcggctc
     3601 ggcgggactc atgctgggtg gcctcgtatg gctgtcggag accgcgtatg attcgtccca
     3661 tgggtggatt cagccggatg gcattcctcg cgctgctcgc gttcgcagtc gcgggatgtg
     3721 actcgcgcgc gaaagcatct gatccgcagg tgcgctcgga gagcaagtcg aaggaatacg
     3781 agagctgcgg cgcgagcctt cactgccagg atgacctgcg gtgcttcgac aacgtgtgcc
     3841 gccgcggagc gcgcagcaac gtcggtgatt acttcgccgc gctcggcgcg cagcagctcg
     3901 cgagaggcga gaccgaagcg tcgatcgact cgtacaacaa ggcgctcggc cactacgaca
     3961 gcgagaagat cggcctgccg cccgatgtcg actgcgcata cggcgccgcg ctcgccgccg
     4021 gcaagcagaa gaaggagcac gccgagctcg ccgcccgcgt cctgcatcgc tgcatcctcg
     4081 cggttcccgt cggcagcgtg ttgcgcacgc aggcgctcgc gcagctggcc ggcatcaccg
     4141 acgcaggtct cgacccgctc tcgctcgggc gcacgcagct cgccgatgtc tacctgacgc
     4201 gcgcgccgca gaagccatcg agcgacaaga tcggcgtcac catcgcggcg aacccaccga
     4261 tcgacaagaa gacgtaccac ttcattccgg accgcctcgc cgagcaagac atgcgcaacg
     4321 cgcttgtcgc ttgctgggag caatacaaca acgcgacgca caaggacacg ctcgtggcgt
     4381 ccgtcggcgt gaaggtcagc tacgtcgcgt cggagtacga agacgagccc ggctcgttct
     4441 cgacaaagct cgagcccgcg gttgcgatgc ccgcaggccc tgaagcgaca gccgaccaat
     4501 gcgttcgcgc cgcggtagag ccggcgatca agggactcgc gactctacgc gacgcgttcg
     4561 cgaccaagct aacgattaca atcaagtgat cgcgggtact gccgcgacct aaaaccattc
     4621 gttcttgcgg ccgatgtaga tgaacgcgcc gaccgcgatg acgccggctg cgatcgatcc
     4681 gatcagcacg gggcgttcct cgacggtctc gcggcccttc acgtacaggt cgcgcgcgcc
     4741 gtccttgccc ttgttcgcga gctgctgggc ttcgaacttg cccttctgca ggaagaactt
     4801 cgccttcgcg ccgtactcct catagagctc ttcagcgcgc agcttgccct tcgcgacggc
     4861 gacgcgtgca cgcgccttga cgtcgacctt ctcctgcacg acatgccgca gctcggcgag
     4921 gctcgcctcg agatcctggc gggcgtcgtg gatttcacgt tcgatgactt cgcgattccg
     4981 catggtgtcc tttcagctgt gagagagcga ttccttgacg cctgcgatcg tgcgcttcgc
     5041 ttcgtatgcg ggcaccgcaa gatccggctt gatgtcgttg ccgagccgct tcgcaaacac
     5101 gcccgcgaca acggcgccgc cgatgagata gatgagcgac atgagaagca ggcgtaccgc
     5161 gagcgagtgg atcaccggct cgagtgcgac gactgcagcc gtgcacaaca tcgcgaagcc
     5221 gatgagcgca acgatgccgc cgaggacgat gatggccgcc tcgccagcag cgagcttggc
     5281 gtgacgcgcg acctcgttct tcgcgagatc gatctcgtcc tttgcgatcg tcttcagatc
     5341 atcggtgatc cgatgcagca gctcgccgac gccatgaccg ctggtttcag caggccgatt
     5401 tggatttaca tagcttccca tggcagtgct cctagagaga tttcccggcg agctgacgcg
     5461 tcgtggcgcg ctgccacata cgaagcaggg cgcgcacccc gagttgctcg gcggtcgccg
     5521 aaacgctttg cagcagggcg tttacagcga gcttcttcat gtcccggaca aagtgcagcc
     5581 aacgtgccgg tagggttgcg agattcccct ttggcgagag cgcgcggtcg tagtacctac
     5641 cgggggtgga aaagcccgat ttatgcgcga tgacgctcgc cgacgccgaa gcgtgggcgg
     5701 tagcgcaggg ctgggcgcgg tttcggggcg agcaagtttg gcgctgggtg cacgatcgca
     5761 acgcgcggtc gttcgaggag atgacgaatc tgcccgccga ggtgcgcacg tcgctcgcgg
     5821 aacgcgcaca gatcggcagc ctcaagatcg cggagattca gaccagccgc gatggcactc
     5881 gtaagctgcg tctcgaaacg cgcgacggcc aatcgatcga gtcggtgctg attccggacg
     5941 gcgaaaagac gacccagtgc atatcgtcgc aggtcggctg cgcggttgac tgccagttct
     6001 gcgcgaccgc gaagatgggc ctgaagcgca atctggacgt cggcgagatc gtcgatcagg
     6061 tgtacctcgc acgccgcctc ctcgcggaag tcgagccggg ccgcaagctc acgaacctcg
     6121 tctacatggg catgggcgag ccgctccaca actacgacaa catggtcaag tcgctgcgca
     6181 tcctctgcaa cgacaagggc gcgaagctcg gccagcgtcg cgtgacggtc tcgacatcgg
     6241 gcctcgtgcc gaagctcgag aagctcggca acgaagatgt tcgcccgaac cttgccgtgt
     6301 cgctcaacgc gccgaacgac gagatccgcg accagatcat gccgatcaat cgcaagtgga
     6361 acatcggcaa gctactcgcc gcgctcaagg cgtatccact cgagcaacgg cgtcgcatca
     6421 cgttcgagta cgtgttgctc gcgaacgtca acgattcgct gcgcgacgcg ggtcagctcg
     6481 ccaagctcct gcgcggcttc cgctgcaagg tgaacatcat cccgtggaac ccgcatcccg
     6541 aagcgccgta cgagcgcccc gcggacgcgg tgatcgaagc gttccaga
//
LOCUS       DATLBZ010000053         4301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3770249, whole genome shotgun sequence.
ACCESSION   DATLBZ010000053 DATLBZ010000000
VERSION     DATLBZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3770249"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VMZ53_02705"
     CDS             complement(<1..662)
                     /locus_tag="VMZ53_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUS27382.1"
                     /translation="MSLSCDEVRQLAADGKADGAFCDWFGAGCTPDEAIFTGPSCSTS
                     ASCAAGSTCIEKHCFAGERELSSIMDRWTHSVETTGNVTQLLDQNSETRAKRLQMMAS
                     AHESIHFTALLIEDDETGRETIAALADAARRGVEVRVIVDATTQYEFSSYDMLKPLVE
                     AGGEILPYNPVVEWALVRWSIGVNANQRLHEKLLIVDGREVVMGGRNVGDDYLLDGKW
                     HDT"
     gene            complement(961..1770)
                     /locus_tag="VMZ53_02710"
     CDS             complement(961..1770)
                     /locus_tag="VMZ53_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS27383.1"
                     /translation="MDPAVLPILTCPRCRAAAPFAHLRHPRCSACGLEPTERHGYLDL
                     IDTSVAGEPTAPTAEQRLMESDLVARVYDRVWRPTFVRMLAGKGAGAAVGGLAGELFI
                     HKHSLSLDDRPGPWLDLSCGPGAFVRALSAAAPGALIVGLDISRAMLDVAAQRTSGYS
                     NAVLVRADAHALPFIDGAFGGVNNAGALHAYDDPELVFREIRRVLRPGGIYVGSTFAE
                     APSLLGRLAARVAGIRRFETSELRAWLQRIGFADYEDVRLGDAFVFRVRRP"
     gene            1840..2670
                     /locus_tag="VMZ53_02715"
     CDS             1840..2670
                     /locus_tag="VMZ53_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27384.1"
                     /translation="MKTRTWLLVGLTCVLSSTGWAQPAKKGGGKKAEKAAKVDLAPVQ
                     AQLAGANQEQAVRAAEQLGQSTDPAAHEALLDALAFGMQPAVAIASIKALSAHPAPAD
                     VNALTRYATHHNPSVRSASFSVLALYPDPTAQKAIVRGLHDMLPAVRAAAAAAAAKGR
                     VRISVEPLMELLARGEQPASAALAAMADVDLARKIGDQLGKISDAMLAQTLGAILKRS
                     DFSSDPARVEIVRAIGKIQGPEAITALTDYIDATPKNPPRQSREEAQKMVDARLGGGK
                     "
     gene            2667..>4301
                     /locus_tag="VMZ53_02720"
     CDS             2667..>4301
                     /locus_tag="VMZ53_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUS27385.1"
                     /translation="MKRLLVAVVLLAACGPKTMFRLSSEDKDNDRAALAKALASRQLP
                     EQASPQNSARQPRVFVVQAGTPKTIVAYDLNAGAVMWQQQADVQSRIWIGGDFILDLE
                     GKSLVARDQKTGAARWKVGVSGAFIGASADKERAYLVWREGNDQKPTWFIAAYDASSG
                     KLIWSHDAEGALGAPVAQSGVVYSPFLTQWLAILDGKTGVQLARLRGIDEQISMVRAT
                     SREAYYGSKQGVFLLDGRSASGKKTEASYGQAKIPTQLDRTTYGPDVYDPVQQGYTAA
                     DRAHVLWAGSSTDSGPMKFTGDTYAVHYFRYIFGFNLDGELAWAYSQPRVELVASDHT
                     GSAIVGMSQNGDIVVLDPKTGAVHARKSLGTAQPVLGATFDADGWAPLASQGDKVETV
                     AALVQIARDRDARFDRVKELAVAALAKMPGAEVTKELLAVLTDKRASVKLKDTVVEML
                     AQRKDPASLPVLTAQLEGHADFIAKSDNDMLTPTAKAIASLGGTNLDGKAVEDALAAL
                     QAHLEDPATTPSDMALVIKAMAAIGGGKERPVLMSHL"
BASE COUNT          762 a         1363 c         1505 g          671 t
ORIGIN      
        1 gtgtcgtgcc atttgccgtc gaggagatag tcatcgccga cgttgcggcc gcccatcacg
       61 acctcgcgac cgtcgacgat caagagcttc tcgtgaaggc gctggttcgc gttgacgccg
      121 atgctccagc gcacgagcgc ccactcgacg acggggttat acggaaggat ctcgccgcct
      181 gcttcgacga gcggcttcag catgtcgtag ctcgagaact cgtattgcgt cgtcgcgtcg
      241 acgatcacgc ggacctcgac accgcggcga gcggcatcgg ccagcgctgc gatcgtttcg
      301 cggcctgtct cgtcatcttc gatgagaagc gcggtgaagt ggatcgactc atgcgcgctc
      361 gccatcatct gcaggcgctt cgcgcgcgtc tccgagttct gatcgagtag ctgcgtcacg
      421 ttgccagtcg tctctacgct gtgcgtccag cggtccatga tcgatgacag ctcgcgctcg
      481 ccggcaaagc agtgcttctc gatgcacgtc gagccagccg cgcacgaagc gctggtcgag
      541 cacgacgggc cggtaaagat tgcctcgtcg ggcgtgcaac cggcgccgaa ccaatcgcag
      601 aacgcgccgt cggccttgcc gtccgctgcg agctgacgaa cctcgtcgca cgagagcgac
      661 atcaaatact cagcggcttc ctgcgcgtcc gcatcgcagg tcggcggcgt gacgtactcg
      721 ccgacacact ctgcgcggtg ctccgctgcc tcgtcgcaaa cgtcgagggc gctaggttcg
      781 tcagcgcagc cgctcgcgat cgcggctagc aacaggagcc atggcgaaga tcgcaacatt
      841 ggcgcactgc gacagcaacg gccgtgccgc tctacatcgc gaaaacgaca aggcatggct
      901 actcgagttg ccggctgtcc cggtcgacag tggggtctta cggccggcgc agaggagcgc
      961 tcacggtctg cgaacgcgga acacgaacgc gtcgccgagg cggacgtctt cgtagtcggc
     1021 gaagccgatg cgttgcagcc aggcgcgaag ctcgctcgtc tcgaagcggc ggatgccggc
     1081 gacgcgagcg gcgaggcggc cgagcagcga gggggcttcg gcgaatgtcg agccgacgta
     1141 gatgccgcct gggcgaagga cgcggcgaat ctcgcggaat acgagctcgg gatcgtcgta
     1201 ggcgtgcagc gcgcctgcgt tgttgacgcc accgaatgcg ccgtcgatga acggaagcgc
     1261 gtgagcgtcg gcgcggacga ggacggcgtt ggagtaacca ctcgtgcgtt gcgccgcgac
     1321 gtcgagcatt gcgcgcgaga tatcgaggcc gacgatgagg gcgcccgggg ccgcggcgga
     1381 gagcgcgcgc acgaatgcgc cgggtccgca ggagagatcg agccaggggc ctggacgatc
     1441 gtcgagcgag agcgagtgct tgtggatgaa gagctcaccg gcgaggccgc cgacggcggc
     1501 gcctgcgcct ttgcctgcga gcatgcgcac gaacgtggga cgccagacgc gatcgtagac
     1561 gcgcgcgacg aggtcgctct ccatcaggcg ctgctccgcg gtgggagcgg tcggttcacc
     1621 cgcgacggag gtatcgatca gatcgaggta gccgtggcgc tccgtcggtt cgaggccgca
     1681 tgcgctgcac cgcggatggc gaagatgggc aaacggcgcg gcggcccggc accgcgggca
     1741 cgtcaggatc ggtagaacgg caggatccac ggtgttggtt ttaacgcacg cgcgatcgca
     1801 acagttgaat cccgacgcgc ttttgggtac gacgctgcga tgaagaccag gacgtggctc
     1861 cttgtagggc tgacgtgtgt cctttcgtcg accggttggg cgcaaccggc gaagaagggc
     1921 gggggcaaga aagcggagaa ggcggccaag gtcgatctcg cgccggtgca ggcgcagctc
     1981 gcgggtgcga accaagagca ggcggtgagg gccgccgagc agctcggtca gtcaacggat
     2041 ccggcggcgc atgaagcgct gctcgatgcg ctcgcattcg ggatgcagcc tgcggtcgcg
     2101 atcgcgagca tcaaggcgct gtctgcgcat cctgcgcccg ccgacgtcaa tgcactcacg
     2161 cgctatgcaa cgcatcacaa cccgtcggtg cgcagcgcct cgttcagcgt gctcgcgctc
     2221 tatcccgatc ccacggcgca gaaggcgatc gtccgcggtc tgcacgacat gcttccggcg
     2281 gtgcgcgctg ctgctgcggc ggcggctgcg aaggggcgcg tgcggatttc cgtcgagccg
     2341 ctgatggagc tgctcgcgcg cggtgagcaa ccggcgtccg cggcgctcgc tgcgatggcc
     2401 gacgtcgacc tcgctcgtaa gatcggcgat cagctcggca agatttccga tgcgatgctc
     2461 gcgcagacac tcggcgcgat cctcaagcga tccgatttct cgtcggatcc ggcgcgagtc
     2521 gagatcgtgc gagcgatcgg caagatccaa ggtcccgagg cgatcaccgc gctcaccgat
     2581 tacatcgatg cgacgccgaa gaatccaccg cggcaatcgc gcgaagaggc ccagaagatg
     2641 gtcgatgcgc ggcttggagg tggcaagtga agcgcctcct cgttgcagtg gtgctgctcg
     2701 cggcgtgtgg cccgaagacg atgttcaggt tgtcgtcgga ggacaaggac aacgatcgcg
     2761 ctgcgctcgc aaaggccctc gcttcgcggc agttgccgga gcaggcgtcg ccgcagaact
     2821 cggcgcgcca accgcgcgtg ttcgtcgtgc aggcgggaac gccgaagacg atcgtcgcgt
     2881 atgacctcaa tgccggcgcc gtgatgtggc agcagcaggc cgacgtgcaa tcgcgcatct
     2941 ggatcggcgg cgatttcatc ctcgatctcg agggtaagtc gctcgtggcg cgcgatcaga
     3001 agaccggcgc ggcgcgctgg aaagtcggcg tcagcggcgc attcatcggc gcgagcgcag
     3061 ataaagagcg cgcgtatctc gtctggcgtg aaggcaacga ccagaagccg acgtggttca
     3121 tcgccgcgta cgacgcgagc tcgggaaagc tgatctggtc gcacgacgca gagggagcgc
     3181 tcggcgcgcc cgtcgcacag tcgggcgtcg tgtattcgcc gtttctcacg cagtggctcg
     3241 cgatcctcga tggcaagacc ggcgtgcagc tcgcccgtct gcgcggcatc gacgagcaaa
     3301 tctcgatggt gcgcgcgacg agccgcgagg cgtactacgg cagcaagcag ggtgtgttcc
     3361 tgctcgacgg gcgctccgca tccggcaaga agacggaagc ctcgtacggc caagcgaaga
     3421 ttccgacgca gctcgatcgc acgacgtacg ggcccgacgt ctacgatccg gtgcagcagg
     3481 gctacacggc cgcggatcgc gcgcacgtgc tgtgggcggg aagctcgacc gacagcggcc
     3541 cgatgaagtt caccggcgac acgtacgcgg tgcactactt ccgctacatc ttcggcttca
     3601 atctcgatgg tgagctcgcg tgggcgtact cgcagccgcg cgtcgagctg gtcgcgagcg
     3661 atcatacggg ctcggcgatc gtcggcatgt cgcagaacgg cgacatcgtc gtgctcgatc
     3721 cgaaaaccgg cgcggtgcac gcacgcaaga gcctcggcac ggcgcagccg gtcctcggcg
     3781 caacgttcga cgctgatggc tgggcgccac tcgcctcgca gggcgacaag gtcgagaccg
     3841 tcgcggcgct cgtccagatc gcccgcgatc gcgatgcgcg cttcgatcgc gtaaaggagc
     3901 tcgccgtcgc agcgctcgcg aagatgcccg gcgccgaagt cacgaaggag ctgctcgcgg
     3961 tgctgaccga taagcgcgcg tccgtgaagc tgaaggacac cgtcgtcgag atgctcgcgc
     4021 agcgcaaaga tccggcgagc ctcccggtgc tcacggcgca gctcgaaggt cacgccgact
     4081 tcatcgcgaa gtccgacaac gacatgctga cgccgactgc aaaggcgatc gcgtcgctcg
     4141 gcggcacgaa cctcgatggg aaggcagtcg aagatgcact cgccgcgttg caagcgcatc
     4201 tcgaggatcc ggcgacgacg ccgtccgaca tggcgctcgt catcaaggcg atggcggcga
     4261 tcggcggcgg caaggaacgg cccgtgctga tgtcgcacct g
//
LOCUS       DATLBZ010000054         4198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311 k141_43309,
            whole genome shotgun sequence.
ACCESSION   DATLBZ010000054 DATLBZ010000000
VERSION     DATLBZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43309"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VMZ53_02725"
     CDS             complement(<1..513)
                     /locus_tag="VMZ53_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HUS27386.1"
                     /translation="MAKVRVGINGFGRIGRGFVRCLAEAKDTFDLVLINDLTDVKTLG
                     HLLKYDSVHGRYPGTVEVSDGGLVINGDMVKISATKDPAAIGWKDLGVDIVIESTGVF
                     VDKAGAGKHIQGGAKKVIISAPAKEPDVTLCMGINTEMYKPGEHHVISNASCTTNCLA
                     PVAKVLHETFG"
     gene            complement(665..850)
                     /locus_tag="VMZ53_02730"
     CDS             complement(665..850)
                     /locus_tag="VMZ53_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27387.1"
                     /translation="MNDREAKARAVKILAKSIYRDLEAQGFDEKQIVALATELISEVT
                     TKIARGSHHEIKQTQVA"
     gene            complement(1053..1457)
                     /locus_tag="VMZ53_02735"
     CDS             complement(1053..1457)
                     /locus_tag="VMZ53_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27388.1"
                     /translation="MLQAVEAPLLKMAAVAGAGLLVAAFAGQTRCHHHDGVVQRLSLV
                     APEEPNALYLTAWSNGDIYVTLHDGKMKSMEFQTRAFINDGCEWLATETLVPDGANRY
                     AYSYDETILSCEPGSTPARKTPRTGYAFVIAE"
     gene            complement(1508..3043)
                     /locus_tag="VMZ53_02740"
     CDS             complement(1508..3043)
                     /locus_tag="VMZ53_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HUS27389.1"
                     /translation="MSFWSVLWDHVWGSHTPFVVVAFFVVRALAARVPKLERYHLRAI
                     GFFLAGHVVTMTIAAAQDAYGYESNLLATTSFSFESLAIATLGVTGLFRVLLPLAGLR
                     LSRILIDLVTAVAVIVVFIAVGKRVGFSVAGLITTSAVLTAVIGFSLQDTLGNVMGGL
                     SVQLDKSIAVGDWVSLGPNQPTGRVTEIRWRYTSIETRDWDTVIVPNGMLVKSTVTII
                     GRRGGSPMETRRRIDFFVDFRTPPTEVISAVEAALRKDTVEGASPDPQPHVVFMGIRD
                     SYAHYQARYWLLDIERDDGTDSKVRIRIYFALRRADIAMAIPASALFVTHETPEREAR
                     KADRELDQRLRALSKVDLFSGLTTTLQRALADQLELAPYAAGESITREGERDDGLFII
                     VEGDAVVRIGIGSTGREVARLGAGQFFGEMSLMTGEARTASVLAATDLVCYRMSKTAF
                     ELVLRETPSIADQIAEVLAMRRNALSAARDERESDRRKRMETAKQDLLGRIRGFFGLD
                     AAR"
     gene            3225..3872
                     /locus_tag="VMZ53_02745"
     CDS             3225..3872
                     /locus_tag="VMZ53_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27390.1"
                     /translation="MRRGLIVIASLLGACDPLADGDYVGDPMFTLQGTLVTTTKQSST
                     AGGVLIAWQDPGGAGGPGVATTPVSVETELPATVKLSVPLPPPPVVRFAFDDSDVELA
                     EGFIVLVDETAGPRPVSQGIARDRVVVFATADVAEGTQAADYLGGPITAGYHLRRLVQ
                     AGPSAAAAPHVSAAQQLMIDRCVASGASLAACTARRSYQLAAVDDTDLVRIVVRP"
     gene            3869..>4198
                     /locus_tag="VMZ53_02750"
     CDS             3869..>4198
                     /locus_tag="VMZ53_02750"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS27391.1"
                     /translation="MMSAHALDALYREHGHAVLRRARRLLRDEDAARDVVQDVFLDVF
                     HKPEAFEARSSIATYLYAATTHACLNRLRNTKTRARIVAIETAARRSIAAPHAETRAI
                     ASELLAAL"
BASE COUNT          713 a         1378 c         1382 g          725 t
ORIGIN      
        1 gccgaacgtc tcgtgcagaa ctttggcgac gggcgcgagg cagttcgtcg tgcacgacgc
       61 gttcgagatc acgtggtgct cgcccggttt gtacatctcg gtgttgatgc ccatgcagag
      121 cgtgacgtcg ggctccttcg cgggcgcgct gatgatgact ttcttggcgc cgccttggat
      181 gtgcttgccc gcgccagcct tgtcgacgaa cacgccggtc gactcgatca cgatgtcgac
      241 gccgaggtcc ttccagccga tcgcggccgg atccttcgtg gcgctgatct tcaccatgtc
      301 gccgttgatg acgaggccgc cgtcgctgac ctcgaccgtg ccgggataac ggccgtgcac
      361 ggagtcgtac ttgagcaggt gcccgagcgt cttgacgtcc gtcaggtcgt tgatgagaac
      421 cagatcgaac gtgtccttcg cctcggcgag gcaacgaaca aatccgcggc cgatgcggcc
      481 aaatccattg atgccaacgc gaaccttagc catggcgcgc accgtagatc acggaagcac
      541 tcagtgccag ggccaggacg cgcgagccgg cgaaggaagc ggtgagacgc aagggagtca
      601 cgctcaccga aaacgaaaac agaccggcga gccggtccgt ctcatcgcag caactgcgat
      661 cgcgttacgc gacctgcgtc tgcttgattt cgtggtggct gccgcgcgcg atcttggtcg
      721 tcacttcgga gatgagctcg gtcgcgaggg cgacgatttg cttctcgtcg aagccttgtg
      781 cctcgaggtc gcggtagatc gatttcgcga ggatcttcac cgcgcgggct ttggcttcac
      841 ggtcattcat agggacacgg tgcgtttcag cacagcgcgt gccagacaca ctgcgatcca
      901 agtctatgaa ttcacaggtg gtactttctg ctgacgaccg attccagtgc gtaatcgcgc
      961 aggtaggtgc gtagggacct tcgcacacgg tcggaccacc ggacaaatcc agagaattca
     1021 ccgagatcgc ggagcgcgtc gcagcttacg agctactcgg cgatgacgaa cgcgtagccg
     1081 gtgcggggcg tcttgcgtgc gggcgtcgag ccgggctcgc acgagaggat tgtctcgtcg
     1141 tagctgtagg cgtagcggtt cgcgccgtcg ggcacgagcg tctcggtcgc gagccactcg
     1201 caaccgtcgt tgataaacgc gcgcgtctgg aactccatcg acttcatctt gccgtcgtgg
     1261 agcgtcacgt agatgtcgcc gttgctccac gccgtcaggt agagcgcatt tggctcttcc
     1321 ggtgcgacga gcgagagccg ttggacgact ccatcgtggt ggtggcatcg cgtttggccg
     1381 gcgaacgccg cgacgagaag gcccgcccct gcaaccgccg ccatcttgag aagaggagct
     1441 tcgacagctt gcagcatcac tcaccatcgt gacgcctccg ccgacgatgt gccagcgaat
     1501 ctttcgacta gcgcgctgcg tcgaggccga agaatccgcg gatgcgaccg aggaggtctt
     1561 gcttcgcggt ctccatgcgc ttgcggcgat cactctcgcg ctcgtcgcgc gcggccgata
     1621 gtgcgttgcg tcgcatcgcg aggacttcgg cgatctggtc ggcgatcgac ggcgtctcgc
     1681 gcaggacgag ctcgaacgcg gtcttgctca tgcggtagca gacgagatcg gtcgccgcga
     1741 gcaccgacgc ggtgcgtgct tctccggtca tgagcgacat ctcgccgaag aactggccag
     1801 caccgaggcg tgcgacctcg cggccggtcg agccgatgcc gatgcgaacg acggcatcgc
     1861 cttcgacgat gatgaacagg ccgtcgtcgc gttcgccctc gcgcgtgatc gattcgcccg
     1921 ccgcgtacgg tgcgagctcg agctggtcgg cgagcgcgcg ctgcaacgtc gtggtgagcc
     1981 cgctgaacag atcgactttg ctcagcgcac gcaggcgttg gtcgagctcg cgatcggctt
     2041 tgcgcgcctc gcgctccggt gtctcgtgcg tgacgaacag cgcgctcgcg ggaattgcca
     2101 tcgcgatgtc cgcgcgccgc agcgcgaagt agatgcgaat gcgcaccttg ctgtccgtgc
     2161 cgtcgtcgcg ctcgatatcg agcagccaat agcgcgcttg atagtgcgcg taactatcgc
     2221 ggatgcccat gaacacgaca tgaggctgcg gatcgggcga tgcgccctcg acggtgtcct
     2281 tgcgcagcgc cgcctcgacc gcgctgatca cctcggtcgg tggcgtgcgg aagtcgacga
     2341 agaaatcgat gcggcggcgc gtctccatgg gcgatccgcc tcgccgcccg atgatcgtga
     2401 cggtgctctt cacgagcatg ccgttcggta cgatgacggt gtcccagtca cgcgtctcga
     2461 tcgacgtgta gcgccagcgg atctcggtca cgcggccggt cggctgattc ggtccgagcg
     2521 agacccaatc tccgacggcg atcgacttgt cgagctggac ggacaggccg cccatcacgt
     2581 tgccgagcgt gtcctgcagc gaaaagccaa tgaccgcggt gagcacggcg ctcgtcgtga
     2641 tgaggcccgc gacggagaag ccgacgcgct tgccgaccgc gatgaacacg acgatcaccg
     2701 cgaccgcggt gacgagatcg atcagaatgc gcgacagccg caggcccgcg agcgggagca
     2761 gcacgcggaa caatcccgtg acgccgagcg tggcgatcgc gagcgactcg aacgagaagc
     2821 tcgtcgtggc gagcaggttc gattcatagc cgtacgcatc ctgcgcggcg gcgatcgtca
     2881 tcgtgacgac gtgaccagcg aggaagaaac cgatcgcgcg caggtgatag cgctcgagct
     2941 tgggcacgcg agcagcgagc gcgcgcacga caaagaacgc gaccacgacg aacggcgtgt
     3001 gcgatcccca aacgtgatcc cagagtacgg accagaagct cacgaaccct cattatgagg
     3061 ctgtccgcgt ccggactcac ggcccgtcga cctcaaaccc gctcgtgcga gtcctccgta
     3121 ggtgcgacag cccagaggat gtcgtagcgc gttcagtcgc agggcgtcag agcgggttat
     3181 cgcgtgctcg tgccggcgcg gcggcgttgc ggtcctatgc cgatatgcgc cggggactga
     3241 tcgtgatcgc gagcctactc ggcgcgtgcg atccgctcgc cgatggcgac tacgtgggcg
     3301 acccgatgtt cacgctgcaa ggcacgctcg tgacaacgac gaagcaatcg tcgacggcgg
     3361 gtggcgtgct catcgcctgg caagacccgg gcggggcagg tggccctggc gtcgcgacaa
     3421 cgcctgtctc ggtcgagacc gagctccccg cgaccgtgaa gctctccgtt ccgctgccgc
     3481 cgccgcccgt cgtccgcttc gcgttcgacg actcggacgt cgagctcgcc gagggcttca
     3541 tcgttctggt cgacgagacc gccggacctc gaccggtgtc gcagggcatc gcgcgcgatc
     3601 gcgtggtcgt gttcgcgacg gcggacgtgg ccgaaggcac gcaagcggcc gactacctcg
     3661 gcggtccgat caccgcgggc tatcacctgc ggcggctcgt ccaagctggc ccttccgccg
     3721 cggccgcgcc gcacgtatca gctgcgcagc agctcatgat cgatcgctgc gtcgcgagtg
     3781 gcgcgtcact cgcggcatgc accgcgcgtc gcagttatca gctcgcggct gtcgacgaca
     3841 cagacctcgt gcgaatcgtg gtgcgcccat gatgagcgcg cacgcgctcg acgcgctcta
     3901 ccgcgagcac ggccacgcgg tcctgcgccg cgctcgccgc ctgctccgcg acgaagatgc
     3961 cgcgcgcgac gtcgtccaag atgtcttcct cgacgtcttt cacaagcccg aagcattcga
     4021 agcgcgcagc tcgattgcga cctatctcta cgccgcaacg acacacgcgt gcctcaaccg
     4081 gcttcgcaac acgaagacgc gtgcgcggat cgtcgcgatc gagaccgccg cccgccgctc
     4141 catcgccgcg ccccacgccg aaacgcgcgc gatcgcctcg gagctgctcg ccgcgctc
//
LOCUS       DATLBZ010000055         4624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5261249, whole genome shotgun sequence.
ACCESSION   DATLBZ010000055 DATLBZ010000000
VERSION     DATLBZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5261249"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1464
                     /locus_tag="VMZ53_02755"
     CDS             61..1464
                     /locus_tag="VMZ53_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HUS27392.1"
                     /translation="MTAPKRQLGLGITAAIVVANMIGTGVFTSTGFQAKGLGDPKTIL
                     IAWVVGGIVALCGAACYAELGTLYPKAGGEYVYLREAYHPVLGFLSGWVSMTAGFSAA
                     IATSALLFSDYLTALFDQLAHRTPDQPHVLGVAPKFIAAALIVGVTCMHAFDTKIGGR
                     VQQVFTIMKVLLIIGFVAAGLGSGNGDWNHFATQHGGYSNIATTSFAMTLMYVSFAYS
                     GWNAAAYIAGEVDKPEKTIPRALLVGTGAVMVLYVLLNVVYFYAVPSDVLAGPPNKFA
                     PIKEVGNAAAIVLFGTTGGNLITSLIAIALVSAVSAMVMAGPRVYAAMAEDHALPKQL
                     AWYSKRGVPTVAVVVQGVLGIAFVMLGDLGSLIRFAAFTLAIFAALTVAALFIVRMDK
                     KLKGPYKTFGYPVTPVLFIATSIWIAYAQIKEHPKESLVVLGVLVFGAVVYVLAVKPP
                     PKPNAPANLPEARVVDE"
     gene            1477..2313
                     /locus_tag="VMZ53_02760"
     CDS             1477..2313
                     /locus_tag="VMZ53_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27393.1"
                     /translation="MRRAAFAVTLAVASGALAAPATQPSLTWADWVGDWNGKLTWSGC
                     AVDDEKRASIQVDATDGSIAVDLTSAGGALGKMTLLENGEGFAAKEGDISLRLTRKDA
                     ALELAVDLDSGCQLRGTLSRSSVGIAACDQLAAWARIEQQCTKLSRPALENPTRLARQ
                     RAEWKKATGDARTKLAEKCTSRAARVQSQLVDVGCAPNPDPNIGMRGAECQALRGVSA
                     RVQRCSNVPFDVRTSLEREVVVLLAAMQGADQASIPVVDAECKREREKVFAIAQQAGC
                     PP"
     gene            2368..3735
                     /locus_tag="VMZ53_02765"
     CDS             2368..3735
                     /locus_tag="VMZ53_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27394.1"
                     /translation="MTDDPAMMLRAAGPGSVATWVRWLVASACVVTVLALLIGGNLLV
                     RELAWFDEQSQGQFWMRRGIAYLAMFAIIGCLLPGSRLSRFLHAAIVLPILHAVLVVI
                     GWNLWQHELPMLSNMSDGRAFAAWFPFSKVALIAIPSTALVAGVIARRRKAEWAHAFT
                     MLSLSMLLLVGLWMPIALGLWGNAPGDTWVTDPVVEDYRVSYFKTLLPHPLEMTLLIV
                     IPPIVAGIAYTTLSLRRSAWVRAHRKGFIIATSILLGLAILFRMGAAASPMVLYSNFV
                     PLLLVGVLVAVSALVALAFSMLMRGYRLRRRFISQERMVGVVVADSKEPIFGFEIPSW
                     LRGPRLVQRTFAISTSQGTIPVTGAELVAPLPTGTTLLGRDETLGLIHPGDTVIIAGQ
                     TEDAGGPFRSTTAPLAGSLYVAPADVERAGLVSAALAMWRPCVAYLLIVTAVALPGLA
                     ALLSG"
     gene            3776..>4624
                     /locus_tag="VMZ53_02770"
     CDS             3776..>4624
                     /locus_tag="VMZ53_02770"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HUS27395.1"
                     /translation="MRSIWGVVACLALAAPSTAEAFCGFYVAGSDQQLVNDATQVVLV
                     RNGTRTVLSMQNNYKGPPDAFAMVIPVPSVLHEGDVKTLPKEVFKHVEQMGAPRLVEY
                     WEQDPCEQAVRDYLERNKVVAEPSAADMAVAGSAADLGVKIEAKFVVGEYQIVILSAK
                     DSTGLDTWLRQEHYNIPAGAEPLLRPYVESGMKFFVAKVDPKKVTFENGRAALSPLRF
                     HYDAEEFALPIRLGLANSSGTQDLIVNILAPGQRYEVANYPNVTIPTNIELKESARDK
                     FGAFYAA"
BASE COUNT          736 a         1494 c         1513 g          881 t
ORIGIN      
        1 cgccgctgct ggcgcgatgc gctcgcgttc ctcgatcgcc acgttcgata ggtctgttcc
       61 gtgacagcac cgaagcggca actcggcctc ggcatcacgg ccgcgatcgt cgtcgcaaac
      121 atgatcggca ccggtgtgtt cacgagcacc ggttttcaag cgaagggcct cggcgatccg
      181 aagaccatcc tgatcgcgtg ggtcgtcgga ggcatcgtcg cgctgtgtgg cgcagcttgc
      241 tacgcagagc tcggcacgct gtatccaaag gccggcggcg agtacgtcta cctgcgcgag
      301 gcatatcacc cggtgctggg cttcttgagc ggctgggtgt cgatgaccgc ggggttctcg
      361 gccgcgatcg cgacgtcagc tctgttgttc tcggactatc tgaccgcgct gttcgaccag
      421 ctagcgcacc gaacgccgga ccaaccgcac gtcctcggcg ttgcgccgaa gttcatagct
      481 gcggccttga tcgtcggtgt cacgtgcatg cacgcattcg acaccaagat cggcggccgt
      541 gtgcaacagg tcttcacgat catgaaggtc ctcctgatca tcggcttcgt tgctgctggc
      601 ctcggctctg ggaacggaga ctggaaccac ttcgcgacgc agcacggcgg ctactcgaac
      661 atcgcgacga cctcgttcgc gatgacgctg atgtacgttt cgttcgcgta ctccggctgg
      721 aatgcggcgg cgtacatcgc tggtgaggtc gacaagcccg agaaaacgat tccacgcgca
      781 ctcctcgtcg gcactggagc ggtgatggtg ctctacgtgc tgctgaacgt cgtctacttc
      841 tacgccgttc catcggacgt tctcgccggc ccgccaaaca agttcgcgcc gatcaaggaa
      901 gtcggtaatg cagccgcgat cgtgctgttc ggcacaaccg gcggaaatct gatcacgagc
      961 ctcatcgcga tcgcgcttgt ctccgccgtc agcgcgatgg tcatggccgg gccccgcgtg
     1021 tacgcggcga tggccgagga tcacgcgttg ccgaaacaac tcgcgtggta ctcgaagcga
     1081 ggcgtgccga cggtcgcggt cgtcgtgcaa ggcgtcctcg gcatcgcgtt cgtgatgctc
     1141 ggcgatctcg gctcgctcat ccgatttgca gcattcacgc tcgccatatt cgcagccctc
     1201 acggtcgctg ccttgttcat cgtccgtatg gacaagaaac tgaagggtcc ctacaagacc
     1261 ttcgggtacc ctgtgacgcc cgtcctgttc atcgcgacct cgatctggat cgcgtatgcg
     1321 caaatcaagg agcacccgaa ggagagcctc gtagttctcg gggtcttggt gttcggagcg
     1381 gtcgtgtatg tcctcgcggt gaagccgccg ccgaagccga acgcgcccgc gaatttgccg
     1441 gaagcgcgcg tcgtcgacga gtgatacgtt tcggtgatgc ggcgcgccgc tttcgccgtc
     1501 actctcgccg ttgccagtgg cgcgctcgct gcgcccgcga cgcagccgtc gctgacgtgg
     1561 gcggactggg tcggcgactg gaacggcaag ctcacgtggt cgggctgcgc ggtcgacgac
     1621 gagaagcgcg cgtcgatcca agtcgatgca accgatggct cgatcgcggt cgatctcacc
     1681 tcggcgggcg gcgcgctcgg caagatgaca ctgctcgaga acggcgaggg cttcgctgcg
     1741 aaagaaggcg acatcagcct gaggctcacg cgcaaagacg cggcgctcga gctcgctgtc
     1801 gatctcgatt cgggctgtca gctgcgcggc acgctatcgc gttcgagcgt cggcatcgct
     1861 gcgtgcgatc agctggcggc gtgggcccgc atcgagcagc agtgcacgaa gctctcgcgt
     1921 cccgcgctcg agaatccaac gcggctcgct cggcaacgcg ccgaatggaa gaaggcgacg
     1981 ggcgatgcgc gcaccaagct cgcggagaag tgcacctccc gtgcggcgcg cgtgcagtcg
     2041 cagctcgtcg acgtcggctg cgcgcccaat cccgatccga acatcggtat gcgtggggcg
     2101 gagtgccagg cattgcgcgg tgtgtcggcg agggtccagc ggtgctcgaa cgtgccattc
     2161 gacgtccgga ccagcctcga gcgcgaggtc gtcgtgctgc tcgccgcgat gcaaggtgcc
     2221 gatcaggctt cgattccggt cgtcgacgcc gagtgcaaac gcgagcgcga gaaagtgttc
     2281 gcgatcgcac agcaggccgg ctgtcctcct tgagatagcc gcaactggaa tagtcgtccg
     2341 gctgtctcat gttccggtat gacgcacatg acagatgacc cggcgatgat gctgcgcgcg
     2401 gccggccccg gttcggtcgc gacctgggtt cggtggctcg tcgcaagcgc atgcgtcgtc
     2461 accgtcctcg cgctgctcat cggcggcaac ttgctcgtcc gcgagctcgc ctggttcgac
     2521 gagcagtcgc agggccagtt ctggatgcgg cggggcatcg cgtatctcgc catgttcgcg
     2581 atcatcggct gcttgctgcc aggatcgcgg ctctcgcggt ttctgcacgc cgcgatcgtg
     2641 cttccgatcc tgcacgccgt gctcgtcgtg atcggctgga acctgtggca gcacgagctg
     2701 ccgatgctct cgaacatgag cgatggccgc gcgttcgccg cgtggtttcc gttctcgaag
     2761 gtcgcgctca tcgcgattcc gtcgaccgcg ctcgtcgcgg gtgtgatcgc gcgtcgtcgc
     2821 aaagcggagt gggcgcatgc gttcacgatg ctgtcgctct cgatgctgct gctcgtcggc
     2881 ctgtggatgc cgatcgcgct cggcctgtgg ggcaatgcgc ccggtgacac gtgggtcacg
     2941 gatccggtcg tcgaggacta tcgcgtctcg tacttcaaga cgctcttgcc acacccgctc
     3001 gagatgacgt tgctcatcgt catcccgccg atcgtcgccg gcatcgcgta cacgacgttg
     3061 tcgctgcgtc gctcggcgtg ggtccgcgcg catcgcaaag gcttcatcat cgcgacgtcg
     3121 atcttgctcg gcctcgcgat cctgtttcgg atgggtgcgg ctgcgagccc gatggtgctg
     3181 tattcgaact tcgtcccgct gctcctcgtc ggcgtgctcg tcgccgtcag cgcgctcgtc
     3241 gcgctcgcat tctcgatgct gatgcgcggc tatcgtctgc gccggcggtt catctcgcag
     3301 gagcggatgg tcggtgtcgt cgtcgccgat tccaaggagc cgatctttgg cttcgagatc
     3361 ccgagttggc tccgcggtcc tcggctcgtg cagcgcacgt tcgcgatctc gacctcgcag
     3421 ggcacgatcc cggtgaccgg tgcggagctc gttgcgcccc tgccgacggg cacgacgcta
     3481 ctcgggcgcg acgagacgct aggcctcatc catcctggtg acacggtgat catcgccggt
     3541 caaaccgaag atgcaggcgg accgttccgc tcgacgacag caccgctcgc gggctcgctc
     3601 tatgtcgcgc ccgccgatgt cgaacgcgcg gggctcgtga gcgcagcgct cgcgatgtgg
     3661 cggccttgtg tcgcgtatct attgatcgtc accgcggtcg cgctcccggg cctcgctgca
     3721 cttctcagcg gctagtccca aggacctgca ggggcagctg gtctaagctg ccacgatgag
     3781 atccatctgg ggggtggtgg cctgcctggc gctcgccgcg ccgagcacgg cagaagcgtt
     3841 ctgcggcttc tacgtggcgg gcagcgatca gcagctcgtc aacgacgcga cgcaggtcgt
     3901 gctcgttcga aatggcacgc gcaccgtgct gtcgatgcag aacaactaca aagggccacc
     3961 cgacgcattc gcgatggtca tccccgtgcc cagtgtcctg cacgaaggcg acgtcaagac
     4021 gctgcccaaa gaggtgttca agcacgtcga gcagatggga gcgccgcggc tcgtcgagta
     4081 ttgggagcag gatccctgcg agcaggcggt gcgcgattac ctcgagcgca acaaagtggt
     4141 ggcggagcct tcggccgcgg acatggcggt cgccggaagc gcagcggacc tcggcgtgaa
     4201 gatcgaggcg aagttcgtcg tcggcgagta ccagatcgtc attctctcgg cgaaggactc
     4261 gacgggcctc gacacgtggc tgcgccaaga gcactacaac attcccgcgg gcgccgagcc
     4321 gctgcttcgg ccgtatgtcg agagcggcat gaagttcttc gtcgcgaagg tcgatccgaa
     4381 gaaggtcacg ttcgagaacg gccgcgccgc actctcaccg ctgcggtttc actacgacgc
     4441 cgaggaattt gcgctgccga ttcgtctcgg ccttgcgaac tccagcggca cgcaggatct
     4501 gatcgtcaac atcctcgcac cgggccagcg ctacgaagtc gcgaactatc cgaacgtcac
     4561 gatcccgacg aacatcgagc tcaaggaatc cgcacgcgac aagttcggcg cgttctacgc
     4621 cgcg
//
LOCUS       DATLBZ010000056         8418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3771514, whole genome shotgun sequence.
ACCESSION   DATLBZ010000056 DATLBZ010000000
VERSION     DATLBZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771514"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VMZ53_02775"
     CDS             complement(<1..270)
                     /locus_tag="VMZ53_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27396.1"
                     /translation="MAKSLKTMRDAAVHALLHDAPKAGPAVDAFLATAAKVPYGGFSG
                     QRETYELEVLHYAMPKARRKTGPLARRMWTQLVTDWTASAPKAGQQ"
     gene            complement(302..1651)
                     /locus_tag="VMZ53_02780"
     CDS             complement(302..1651)
                     /locus_tag="VMZ53_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27397.1"
                     /translation="MRPSVCAWLLVAACGRIDFAPLPDAGAPPVTNGRRLHAVEAESD
                     HGSLFLGWYDPELGTECTFRTAEDGATRCLPPTDYGVLFADAGCTAPLAELVGPWANA
                     ACARPPYATRLATNTVYRMGMASTQTTTYAGNPTNCLVVMPAITPPTFLFEVGEVIPA
                     SAFVAATEALVPLGRLSAVELIGDDDSHQRTGEMFDTVRNERCRWLANGIDTAYCVGN
                     SVTVSPPGFSDAACTQPAVRASAPGSFVLIVDASACPTSQRMVATGPALGAFYRNQGN
                     TCVVDAGTAYSIVDVPPDGPVRASLVFDRNAGAGVLASWHTDELDVPASLFDTKRNSA
                     CTPFVDHASKRYVCAPPTTTAYGVYSDAACTQLTFEFTSPCNDANHAFSNYPQPPSGS
                     AEICEGAGMDLQVFDTPLPTPRYEDNGIGCVQITGWGILNPTPLDGTQLPRLRRVIR"
     gene            1767..2174
                     /locus_tag="VMZ53_02785"
     CDS             1767..2174
                     /locus_tag="VMZ53_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27398.1"
                     /translation="MKTTSSSVLRALAAFALVSACTADVAIRTQAEKNLPEACTANAP
                     DPRACDPADTKKTTICHVPPGNPANAHTICVGNAAVPAHLEHHADFIGTCSCGDGGDG
                     SGGGGGGGGGGGGDGSGGGGGSGTGDGGGGGLY"
     gene            complement(2211..4556)
                     /gene="bamA"
                     /locus_tag="VMZ53_02790"
     CDS             complement(2211..4556)
                     /gene="bamA"
                     /locus_tag="VMZ53_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HUS27399.1"
                     /translation="MKCLGFAFAGCLLAMGLAHAEPAIEQIGVRGNHRMEAEVIRLAV
                     ASKVGAPLVPEKLAADVRSIWKLGAFSDVQVVSETGQSGAIVTFEVVERPTIRKVLVA
                     GNDDVELDKINEVLDLHTDEVADFTAIHRNRDHIAELYEARGYMFATVDAQMVPAGTG
                     EIDVHYTIDEHAKVKVADVTFVGNRSISSKDLREHMATRTPGALSFLNGSGVFRRNDL
                     DRDLAVLTGYYLDRGFATVKISPPQLRLSRDHKRMTVTIEINEGPRFTYGAVEVKGDL
                     LGSTADNLALLHTRPGTTFARAALEADRKALEIHYQDLGYARASVALRPQFDLPNRRI
                     ALSFEVTRGKRVYVERIHIRGNSKTRDKVIRREMRIAEGELYSGTRIERSRAQIVALG
                     YFEDVTVSTQNGSSEDFIDVNVEVRERPTGTFQLGAGFSSQEKFIVQGQVAMQNLGGR
                     GESLQAQVAFSGLRRLFMLRFTEPYFLDTRWIATAEAYNQSRGFGWYSRTATGGALTW
                     GYPISDRINAFLTYRLENVSISGASGGIASLGATAAPLALDTANLFRGGWTSSVRGTM
                     SYDTRDNRLFPTQGYYATAFAEYAGRVTGSENQYVRWGGFARRYQHLGPFVLRLNAEL
                     GVTTSLDGRGVPLTERYLLGGITDIRGYAPRSLGPRLWSQRPGDVGQDLAPLAIGGNV
                     QVIGNAELEFPIVKKLGLSGVAFFDLGNAYNLESRFCNRPGTDACPSAIEMVSGLRKS
                     VGVGVRWLSPLGPLRFEWGLPLDLRPGEKPAGLEFTIGGSF"
     gene            4694..5998
                     /locus_tag="VMZ53_02795"
     CDS             4694..5998
                     /locus_tag="VMZ53_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HUS27400.1"
                     /translation="MRVLGIGDYNDLGSVYIQLAREGHEVRVHIAEPGSRDVLAGLVT
                     TTDDWRAELPWIREAGVDGLILFEEVGRGALQDELRRDGYHVLGGSAYGDRLEQDRAF
                     GQKTLAGIGLTTLKTHELDNLDEGIALIQREPRQYVLKFNGSWLTSDMNYVGMRADGA
                     DVISALRRYRLLDPEQHEPTSFVLMDHVRGVETGLGAFFDGEKFVGPVNLDWEHKRFF
                     PGDLGELTGEMGTVVTYRGGERLFEATLAKLAPALRQARHVGYVNLNTIINADGVWPL
                     ELTCRFGYPGFAILSALFAEPMGPMLSRVARGEAAEIKTHDGYAVGVVLTVPPFPYHH
                     GYDELSKGIPILLDDLNDDERANLHFGEVAMQSGELVTAGQIGYIMVVTGRGSTLEAA
                     QRAAYRLAAKIAIPNVRYRNDIGNALRTSGLAELTRLGWLAP"
     gene            complement(5970..6848)
                     /locus_tag="VMZ53_02800"
     CDS             complement(5970..6848)
                     /locus_tag="VMZ53_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUS27401.1"
                     /translation="MLEALHRQLAARNSDRLTARLPGPGWRDELAHEHAVRLLEGEIL
                     ELERAEIADRAATAQRDADAFIAWFEELERTGPGQYDPLFAWIEHDASLEQMRWFLHQ
                     EVAGEAGFDDLVALTQIKLPDRAKLELARNYWDEMGRGNADGMHGPMLSRLAEELSLA
                     ELAPKIPIVWESLALGNIMLGLAANRRYAYHSLGALGAIELTAPSRARCVNAALKRLA
                     VAPVARHYFALHSTLDVKHSRAWNAEALWPVVSAEPDAARAIAEGALLRLHAGARCFE
                     RYRRELAVTVPASQAA"
     gene            7027..7794
                     /locus_tag="VMZ53_02805"
     CDS             7027..7794
                     /locus_tag="VMZ53_02805"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HUS27402.1"
                     /translation="MNPRRALAQHSALIRVVRALGASVLTVPFVHGAFDSVFAKDNAL
                     YAQRADTTHALFAEPRYRERQAEQSVRRSDLQHAGISIEPATAAFEGGDVVVVPGRFA
                     FLGHGFRSSPAAANTLERFLGLPVIPLELVDPALYHLDTALGILGDGTALACDDAFSP
                     HSREVLRRVCPVMPVSREEALRFAVNFVDVGRAVVTGTDSEEVTEILSSLGRRTVYTP
                     LDEFHRAGGSAACLLAPIYDQPTLAIAATTAIRSTVA"
     gene            complement(7740..>8418)
                     /locus_tag="VMZ53_02810"
     CDS             complement(7740..>8418)
                     /locus_tag="VMZ53_02810"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUS27403.1"
                     /translation="GLMRSSVRFSTLGAGLYVHSAYPTLDENAVFFGPDTYRFCGLVE
                     GMAARRVVDVCCGSGVGGLSLGTRADTIVLADVNPAALRLARVNAHLNGLADRVELVA
                     GDLFAEVTGDVDLVIANPPYLLDPRHRVYRDGGGSVGEGLAVRIVREGLARLSSGGRL
                     VLYTGAPIIDGHDRVREAIAPILDTAAAQWSYRELDPDVFGEELELAHYATVERIAVV
                     AAIASVG"
BASE COUNT         1395 a         2667 c         2908 g         1448 t
ORIGIN      
        1 ttgctggccg gccttcggcg cggatgcggt ccagtcggtg acgagctgcg tccacatgcg
       61 gcgcgcgagc gggccggtct tccgacgagc tttcggcatt gcgtagtgca gcacctccag
      121 ctcgtacgtc tcgcgctgcc ccgagaatcc gccgtacggc accttcgcgg ccgtggcgag
      181 aaatgcgtcg acagcgggtc ccgctttggg cgcgtcgtgg aggagcgcgt gaacggcagc
      241 gtcccgcatc gtcttgagcg atttcgccat tggcggcaac gttatcaccg ccgcgcgaga
      301 gtcaccggat tacgcggcgc agtcgcggca gctgtgttcc gtcgagtggc gttggattca
      361 agatgcccca cccggtgatc tgcacgcagc cgatcccgtt gtcctcgtaa cgcggcgtcg
      421 gaagaggtgt gtcgaacacc tggagatcca tgccggcgcc ctcgcagatt tcggcggagc
      481 ccgaaggggg ctgcgggtag ttcgagaagg cgtggttggc gtcgttgcac ggcgacgtga
      541 actcgaatgt cagctgcgta cacgccgcgt ccgagtagac gccgtacgca gtcgttgtcg
      601 gcggtgcgca cacgtagcgc ttcgacgcat ggtcgacgaa cggtgtgcac gccgagttgc
      661 gcttcgtgtc gaacagcgac gcaggaacat cgagctcgtc cgtgtgccac gacgcgagca
      721 cgccagcgcc cgcgttgcga tcgaacacga gtgacgcgcg caccgggcca tctggaggaa
      781 cgtcaacgat ggagtacgcc gtcccggcat cgacgacgca cgtgtttcct tggtttcggt
      841 agaacgcgcc gagggcgggg ccggtcgcga ccatgcgctg tgacgtcgga caggcactcg
      901 cgtcgacgat caagacgaag cttccggggg cacttgctct cacggcgggt tgggtgcacg
      961 cggcgtcgga gaatccgggc gggctcaccg tgacgctgtt gccaacgcag tacgccgtgt
     1021 cgattccgtt cgcgagccag cggcagcgtt cgttgcgaac ggtatcgaac atctcgccgg
     1081 tgcgctggtg ggaatcgtcg tcgccgatga gctcgaccgc ggagaggcga ccgagcggaa
     1141 cgagcgcttc ggttgcagca acgaatgcac tggcggggat tacctcgccg acctcgaaca
     1201 agaacgtggg cggtgtgatt gccggcatga cgacgaggca gttcgtcggg tttcccgcgt
     1261 aggttgtcgt ctgcgtcgac gccatgccca tgcgatagac ggtgtttgtg gcgaggcgcg
     1321 ttgcgtaggg tggtcgagcg catgcggcat tcgcccaagg gccgacgagc tctgcgagcg
     1381 gagcggtgca accagcatcg gcgaacaaga cgccgtagtc cgtcggtggc aagcagcgcg
     1441 tcgctccgtc ctctgccgtg cgaaacgtgc attcggtccc gagctccggg tcgtaccaac
     1501 cgaggaagag actgccgtgg tcggattcag cctcgaccgc gtggagacgt cggccgttcg
     1561 tcacgggtgg cgcaccggca tcaggaagcg gagcgaagtc gatgcggcca cacgcggcga
     1621 caaggagcca cgcgcagacc gacgggcgca cgccctacga ttgcacgaat gcgcaggaac
     1681 gatcgcgtgc cgaagccaaa cacgctcgtt cagcggactt atgaaataac tgccaccttc
     1741 gcggcgggtg gccccactag ccgtacatga agaccacttc gagctccgta ctgcgtgcgt
     1801 tggctgcgtt cgcgctcgtg agcgcgtgta cggccgatgt tgcgattcgg acgcaggccg
     1861 aaaagaacct gccggaagcg tgtacggcga atgctccgga tccgcgtgcg tgcgatccgg
     1921 cggatacgaa gaagacgacc atctgccacg ttcctcccgg caatccggcg aacgctcaca
     1981 cgatctgcgt aggcaacgcg gctgtcccgg ctcacctcga gcatcacgcc gacttcatcg
     2041 ggacttgctc gtgtggcgac ggtggcgatg gcagcggtgg cggcggtggc ggcggtggcg
     2101 gcggcggcgg tgatggcagc ggcggtggcg gtggtagcgg caccggcgac ggtggcggcg
     2161 gcggattgta ctaacggccg gcagcgcgga cggcacacgc atcgcgcgcg tcagaacgag
     2221 ccgccaatcg tgaactcgag gcctgcgggc ttttcgccgg ggcgcaggtc cagcggcaag
     2281 ccccactcga accgcagtgg gccgagcggc gagagccagc ggacgccgac accgacggat
     2341 ttgcgcaggc cgctgaccat ctcgatggcg ctcgggcacg cgtcggtgcc ggggcgattg
     2401 cagaagcgcg actcgaggtt gtacgcattg ccgagatcga agaacgcgac gccggagagg
     2461 cctagctttt tgacgatcgg gaattcgagc tcggcgttgc cgatgacttg aacgttgccg
     2521 ccgatcgcga gcggcgcgag gtcttggccg acgtcgccgg gacgttgcga ccagaggcgc
     2581 gggcccagcg agcgcggtgc gtagccgcga atgtcggtga tgccgccgag caagtagcgc
     2641 tcggtgagcg ggacgccgcg gccatcgagc gatgtcgtga caccgagctc cgcgttgagg
     2701 cgcaggacga atgggccgag gtgttggtag cggcgcgcga agccacccca gcgcacgtat
     2761 tggttttcgg agcccgtgac gcggcctgcg tattcggcga atgcggttgc gtaatagcct
     2821 tgcgttggga acaggcggtt gtcgcgcgtg tcgtaggaca tcgtgccgcg caccgaggac
     2881 gtccagccgc cgcggaagag gttggcggtg tcgagcgcga gcggggcggc ggtcgcgccg
     2941 agactggcga tgccgccgga ggcaccggag atcgagacgt tctcgaggcg gtacgtgagg
     3001 aaggcattga tgcggtcgga gatcgggtac ccccacgtca gtgcgccgcc cgtagccgtg
     3061 cgcgagtacc agccaaagcc gcggctctgg ttgtacgcct cggcggttgc gatccatcgc
     3121 gtgtcgagga agtacggctc ggtgaagcga agcatgaaga gacggcgcag tccggagaat
     3181 gcgacctgcg cttggagcga ctcgccgcgg ccgccgaggt tttgcatcgc gacttggcct
     3241 tggacgatga acttctcttg ggatgagaag ccggcgccaa gttggaacgt gcctgtcggg
     3301 cgctcgcgga cttcgacatt gacgtcgatg aaatcttcgg agctgccgtt ctgcgtcgag
     3361 acggtgacgt cttcgaagta tccgagtgcg acgatctgcg cgcgcgaacg ctcgatgcgc
     3421 gtgccgctgt agagctcgcc ctcggcaatg cgcatctccc gacggatgac tttgtcgcgc
     3481 gtcttgctgt tgccgcgaat gtggatgcgc tcgacgtaga cgcgcttgcc acgcgtgacc
     3541 tcgaagctga gggcgatccg gcggttcggg agatcgaatt gcggccgcag cgcgacgctt
     3601 gcgcgcgcgt agccgaggtc ttgatagtgg atctcgagcg ccttgcgatc ggcttcgagc
     3661 gcggcgcgtg cgaacgtggt gccggggcgc gtgtgcagca gcgcgaggtt gtcggctgtc
     3721 gagccgagca ggtccccctt cacctcgacg gcgccgtacg tgaaacgcgg gccttcgttg
     3781 atctcgatcg tcacggtcat ccgcttgtga tcgcgagaga ggcggagttg cggcgggctg
     3841 atcttcaccg tcgcgaaccc gcgatcgagg tagtagccgg tgagaaccgc gagatcgcga
     3901 tcgagatcgt tgcggcgaaa gacgccggag ccattcaaga acgacagtgc gccgggcgtc
     3961 cgcgtcgcca tgtgctcgcg caggtccttg ctcgagatgg agcgattgcc aacgaacgtg
     4021 acgtcggcga ctttcacttt cgcgtgttcg tcgatggtgt agtggacgtc gatttcgccc
     4081 gtgccggccg gaaccatctg ggcgtcgacc gtcgcgaaca tgtaaccgcg cgcttcgtag
     4141 agctcggcga tgtgatcgcg gttgcgatgg atcgcggtga agtcagcgac ctcgtcggtg
     4201 tggaggtcga gcacctcgtt gatcttgtcg agctcgacat cgtcgttgcc ggcgacgaga
     4261 accttgcgga tcgtcggacg ctcgacgact tcgaacgtga cgatcgctcc gctctggccg
     4321 gtctcgctca cgacctgcac gtcggagaac gcaccgagct tccagatcga gcgtacatct
     4381 gccgccagct tctctggcac gagcggcgcg cccaccttcg aggcgaccgc aaggcggatc
     4441 acttcggctt ccattcgatg attgccgcgc acgccaatct gctcgatggc tggctctgca
     4501 tgcgccaagc ccatcgcgag cagacagccg gcgaaggcga agcccaagca cttcacactc
     4561 gctccttctg tctctggtct tgtgcattcg gaccaacgcg ccccggttgc ggttgtccgc
     4621 gctcagcatt tcactgctag cagggcctga cgcactcccc ttgtgactcg tacgtgacac
     4681 actgccgtcg acgatgcgcg ttctcggcat tggcgattac aacgacctcg gctcggtcta
     4741 tatccagctc gcgcgcgagg ggcatgaagt ccgcgtgcac atcgcggagc ccgggtcgcg
     4801 cgacgttctc gcggggctcg tcaccacgac cgatgactgg cgcgccgagc tgccgtggat
     4861 ccgcgaggct ggcgtcgacg ggctgatcct gttcgaggaa gtaggccgcg gtgcgctcca
     4921 agacgagctg cgtcgcgacg gctatcacgt gctcggcggc agtgcgtacg gcgatcggct
     4981 cgagcaagat cgggcgttcg gccagaagac actcgccggc atcgggctga cgacactgaa
     5041 gacgcacgag ctcgacaatc tcgacgaggg gattgcgctg atccagcgag agccgcgcca
     5101 gtacgtgctc aagttcaatg gctcgtggct gacgtcggac atgaactacg tcgggatgcg
     5161 cgccgacggt gccgatgtga tcagcgcgct gcggcggtat cgcttgctcg atccggagca
     5221 gcacgaaccg acgagcttcg tgctcatgga tcacgtgcgc ggcgtggaga cgggcctcgg
     5281 cgcgttcttc gacggcgaga agttcgtcgg tccggtgaac ctcgactggg agcacaagcg
     5341 cttctttccc ggcgatctcg gcgagctcac cggcgagatg ggcaccgtcg tcacgtatcg
     5401 cggcggcgag cgactcttcg aagccacact cgcgaagctc gctcccgcgc tgcgccaggc
     5461 gcgtcacgtc gggtacgtga acctcaacac gatcatcaat gccgatggcg tgtggcccct
     5521 cgagctgacg tgtcgcttcg gctacccggg ctttgcaatc ttgtccgcgc tgttcgccga
     5581 gccgatggga ccgatgctgt cgcgcgttgc ccgaggtgag gcagcggaga tcaagacgca
     5641 cgacggctat gcggttggcg tcgtgcttac cgtgccgccg tttccgtacc accacggtta
     5701 cgacgagcta tcgaagggca ttccgatcct gctcgacgat ctgaatgacg acgagcgcgc
     5761 gaacctccat ttcggcgagg tggcgatgca gagtggcgag ctcgtgaccg ctgggcagat
     5821 cggctacatc atggtcgtca ccggccgcgg ctcgacgctc gaggccgccc agcgggccgc
     5881 gtatcgcctc gccgcaaaga tcgcgatccc caacgtccgt tatcgcaacg acatcggcaa
     5941 tgcgctgcga acgagcgggc tcgccgagct cacgcggctt ggctggctgg caccgtgacg
     6001 gcgagctcgc ggcgatagcg ctcgaagcaa cgcgcacccg cgtgcagccg gaggagcgcg
     6061 ccctcggcga tcgcacgcgc agcgtcgggc tccgcggaga caacgggcca cagcgcttcg
     6121 gcgttccacg cgcgcgagtg cttcacgtcg agtgtggagt gcagcgcgaa gtaatggcgc
     6181 gcgacgggag ccacagcgag tcgcttcagg gccgcgttca cgcagcgcgc gcgacttggc
     6241 gccgtcagct cgatcgcacc gagagcgccg agcgagtgat atgcgtagcg gcggttcgcg
     6301 gcgagcccga gcatgatgtt gccgagcgcg agcgactccc acacgatcgg aatcttcggc
     6361 gcgagctccg cgagcgagag ctcttctgcg aggcgcgaca acatcggccc gtgcatgccg
     6421 tcggcattcc cgcgtcccat ctcgtcccag taattgcgcg cgagctcgag cttggcgcga
     6481 tcgggaagct tgatctgggt cagcgcgacg agatcgtcga agcctgcctc tccggcgacc
     6541 tcctgatgga ggaaccagcg catctgctcg agcgacgcgt cgtgctcgat ccacgcgaac
     6601 agcggatcgt attgtccggg tcccgtacgt tcgagctcct cgaaccatgc gatgaacgca
     6661 tcggcgtcgc gctgcgccgt tgcagcgcgg tcagcgattt cggcgcgctc cagctcgagg
     6721 atctcgcctt cgagcaatcg gactgcgtgc tcgtgcgcga gctcgtccct ccacccgggg
     6781 ccgggcaggc gggccgtgag gcggtcacta ttcctcgctg cgagctgtcg atgcagagct
     6841 tccagcatcc agccaatacc tttgcaaggc caggaccgtg gcgggcggaa cggcgcttgc
     6901 tcgacttccg catagatgcg ctttgttgcg agcacgcctg agtgccctgc gtccgagcac
     6961 gctcgaacgg atgaatgcat ctaccgagtt gcgtgggcaa tcaatccgca catggtcgtg
     7021 ggatcggtga acccccgacg cgcgctcgca caacactccg cgctcattcg cgtcgtgcgg
     7081 gcgctcggcg ccagcgtgct caccgtgccg tttgtccacg gagcgttcga ctccgtgttt
     7141 gcaaaggata atgcgctgta cgcgcaacgt gcggacacaa cacatgcgct gtttgccgaa
     7201 ccccggtatc gcgaacggca ggcggaacag tctgtccggc gatcggacct ccagcacgct
     7261 ggcatctcga tcgagccggc gacagcggcg ttcgagggtg gcgatgtcgt cgtcgtcccg
     7321 ggccgcttcg cgtttctcgg ccacggcttc cggtcgtcgc ccgcggctgc caatacgctc
     7381 gaacgcttcc tcggattgcc ggtgattccg ctcgagctcg tcgatcccgc gctctatcac
     7441 ctcgacaccg cgctcggcat tctcggtgat ggcaccgcgc tcgcgtgcga tgacgcgttc
     7501 tcgccgcact cgcgcgaagt cctgcgtcga gtgtgtcccg tcatgccggt gtcgcgcgaa
     7561 gaagcgcttc gcttcgccgt caacttcgtc gacgttggca gagcggttgt caccggcacg
     7621 gactccgaag aggtcaccga gatcctctcg agcctcggtc gccgcacggt gtacacgccg
     7681 ctcgacgagt tccatcgcgc aggtggcagc gctgcgtgcc tgctcgcgcc gatctacgat
     7741 cagccgacgc ttgcgattgc ggcaacgacc gcgattcgct cgacggtcgc gtagtgcgct
     7801 agctcgagct cttcgccgaa cacatcgggg tcgagctcgc gatagctcca ctgcgcggca
     7861 gctgtatcga ggatcggcgc gatcgcctca cgcacgcggt cgtggccgtc gatgatcggg
     7921 gcgccggtgt agaggacgag ccgcccgccg ctcgagagtc gcgcgaggcc ctcgcggacg
     7981 atgcgaactg caaggccttc tccgacggag ccgccgccgt cgcgatagac gcgatgccga
     8041 gggtcgagca agtacggcgg attcgcgatc accagatcca cgtctccggt gacctccgcg
     8101 aacagatcgc cagcgacgag ctcgacgcga tccgcgagac cattgaggtg cgcattgacg
     8161 cgcgcgagcc ggagcgcggc cgggttcaca tccgcgagca cgatcgtgtc ggcgcgcgtg
     8221 ccgagcgaca ggccacccac gccggagccg cagcacacgt cgactacccg tcgcgccgcc
     8281 atgccttcga cgaggccgca gaaccggtac gtgtcggggc cgaagaagac cgcgttctcg
     8341 tcgagcgtcg ggtaggcgga gtgaacgtag aggccggcgc cgagcgtcga gaaccggacc
     8401 gacgagcgca tcaggccc
//
LOCUS       DATLBZ010000057        25192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311 k141_44188,
            whole genome shotgun sequence.
ACCESSION   DATLBZ010000057 DATLBZ010000000
VERSION     DATLBZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 25192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25192
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44188"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..466
                     /locus_tag="VMZ53_02815"
     CDS             143..466
                     /locus_tag="VMZ53_02815"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUS27404.1"
                     /translation="MFHFRSLDVYRCAVAFLPLAYGMAKELDSEMAGQLRRAALSINL
                     NIAEGTGRFDRDQRRFLVTARGSALECAAVLDAMTALELADERIATGDAFLVRIVSML
                     TKMIG"
     gene            532..1932
                     /gene="asnS"
                     /locus_tag="VMZ53_02820"
     CDS             532..1932
                     /gene="asnS"
                     /locus_tag="VMZ53_02820"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HUS27405.1"
                     /translation="MHGTRIALILAGEVPVGTKVTIEGWVRTRRDSKAGLSFLAIHDG
                     SSHEPLQVIAPQELPNYQDDVLKITTGCAVRATGELVASQGKGQSVEVRADKIEVVGW
                     VDDPDTYPMQPKRHTMEYLREVAHLRPRTNVIGAVTRVRHALAQAVHRFFHEHGFFWI
                     HTPIITASDAEGAGQMFKVSTLDYANLPRTADGKIDFDKDFFGREARLTVSGQLNVET
                     YCMALSRVYTFGPTFRAENSNTRRHLAEFWMIEPEIAFADLNDDANLAEEFLKYIFRA
                     LLTERKDDMAFFEKFVDKECVTRLSKMIESKFARMDYTDAIKELEKSGQKFEFPVKWG
                     MDLQSEHEKYLTEVVIKGPVAVVNYPKDIKAFYMRMNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLEMLDKRITEMGLHVKDYWWYRDLRRYGTVPHAGFGLGFERAIIYATGIEN
                     IRDVIPFPRAPRQAEF"
     gene            1929..2408
                     /locus_tag="VMZ53_02825"
     CDS             1929..2408
                     /locus_tag="VMZ53_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27406.1"
                     /translation="MKDAVLAALADYAAGKTGATRAIAGAIASHFLAVTTARSLALVG
                     GDVSVQSLLAHRTWFDPKDIRCTSGSVAAAVGGRQVPLAEALAADIVCVHEPIAITGV
                     QLRRGTHINALAAVTLDSELRSVATLVDEAGLHKLAAGIIDGRQLDELTVFVYGAVP"
     gene            complement(2390..3199)
                     /locus_tag="VMZ53_02830"
     CDS             complement(2390..3199)
                     /locus_tag="VMZ53_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27407.1"
                     /translation="MSRWRLLAFAFALAGCDSVFSVDHVGPVREDGGGGDADARGPDA
                     MPDGPGACAPNEHDEDSDGFPDRCDGCPTVMDPDDADADDDGVWDNCDQNRGAGGRDA
                     ILFFAAFGGTDLDRFDIAGSVAEDPAGGGVATIANGSIMTKAMFLPTKIEILTAGLTG
                     VDINDEARIDAPNAVCHIRTRTCGTSPTTGSMCVQMDASGGVGGQLGAPSGLLAVNLY
                     RQGSNTFCYITANGMLSDAAPVVFTNGKLRISTTTGSTLSVKSVLVYGTAP"
     gene            complement(3199..4029)
                     /locus_tag="VMZ53_02835"
     CDS             complement(3199..4029)
                     /locus_tag="VMZ53_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27408.1"
                     /translation="MMRTCVLALLLLGGCDSLFNLDHVPPGGGGGSDGSMGDDDAAVR
                     DGDPLMADAADCGTFDEDADGRMDGCDLCPTVAGSEADADLDGLPDACDPDLGTGQKD
                     KILFAALFSDPLELGAKFDSTNTENMFPSSLVYVNGTGALAATMTTKVSLAPTRIVLN
                     ITGVEGGGYSAIVAVSQPSITAEVHAAGCDQTTLTQTCFLTQTGSGFGILQTKASNVT
                     RIELSRNAGGTSTVFAVTASSTTGIGSTAQLSQAPITVTATAISGVKILNMVVYGSGP
                     "
     gene            complement(4026..4364)
                     /locus_tag="VMZ53_02840"
     CDS             complement(4026..4364)
                     /locus_tag="VMZ53_02840"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HUS27409.1"
                     /translation="MADNLDALASNTVSRSVLSEEQLRKEVAALGARWSIDGENLKLR
                     LEGAPMAKYGAAAAHAATLADEMDHHPTIVLEYAAMTLTIHTHDKKAITLLDLVYAAR
                     LERWLRTAGW"
     gene            4388..4900
                     /locus_tag="VMZ53_02845"
     CDS             4388..4900
                     /locus_tag="VMZ53_02845"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HUS27410.1"
                     /translation="MEMYDLTVPQLGLALRNIDGWMIRAVKHADTKQVPHDQLLSARL
                     APDQYPFVRQVQTACDNAKLIAARLAGKQAPGHPDTETTFEQLRARIAVVVEYLTTFT
                     REDFAAAAERKIVLPWMTNGQHMAATDYVTQFGLPNFYFHVVTAYAILRNQGVPLGKN
                     DFLGQITIRT"
     gene            4925..6490
                     /locus_tag="VMZ53_02850"
     CDS             4925..6490
                     /locus_tag="VMZ53_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27411.1"
                     /translation="MTSGIDAGIRVGKYEVGRKLGQGGFGILYVARDHELGREIAIKF
                     LRPEHAFKPTVVQRFLQEARAAARINHPGIVTVFESGLVAGTNTRADGTVFIAMELLA
                     GDTLAQKIRGLGRLPTTLAINYARQLASALGAAHQAGIVHRDLKPPNVFVVPDAAVVG
                     GERLKVLDFGIAKLADDFGSGMQTHSMLMLGTPMYMSPEQCKSSAKVDARSDIYSLGC
                     ILFEMLSGKPPFDGDSGELIAKHQLVPPPTMRSVISDIPVPLDRLIASMLGKSPADRP
                     QTMGLVEDALAEIDSRAVERPSIAGEDLPAMEAYAPTTIEPSAMRKRLPLVAGAAGAA
                     ALGIMAGLLAMCGDKKPAAQANVARDASIIAEAAPDAVELTPDAMELTVTPDAAVAEE
                     PVPPVDHIDPSITPARVTTKSPTKPATKTPAKAKTAKTTDVKPPVNCDADGLKDKGME
                     NVNMGQHAAALAQFEASLRCKPDSYVLALAFMAACNAQNVAKAKSYYLKISPPERERY
                     KVMCVRNHINPGD"
     gene            complement(6491..7516)
                     /locus_tag="VMZ53_02855"
     CDS             complement(6491..7516)
                     /locus_tag="VMZ53_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUS27412.1"
                     /translation="MKYRNLGAAGVKVSSLCLGAMTFGEADEKSFMHKVGSDEATAHA
                     VLDRALAAGINFIDTADVYGQDGLSERVLGNWFEARGTRDKVVLATKFRFTMGEGANR
                     SGASRYRIVKCCEDSLRRLKTDRIDLYQIHMQDTTVPEEETLRALDDLVSAGKVLYTG
                     CSNYAAYRLMNSLWLSRTNRWASFVTLQAQYSLIVRDLEREHVPLCREEGLGILPWSP
                     LAGGFLTGKFERGKAADATTRLGAKPDRMARYDSDRNWKVLDAVKSVAAEVGSTPAAV
                     SLAWLLAKPQVTSVIFGARTIEQLEANLAGAELELPAKHVETLDKASAIDLGYPYTFI
                     AQTQGTW"
     gene            7587..8480
                     /locus_tag="VMZ53_02860"
     CDS             7587..8480
                     /locus_tag="VMZ53_02860"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HUS27413.1"
                     /translation="MSVNAVAAAQSKGWRHNYFNALSIPFWGVHILAIIGIAITGVSW
                     LGLALVAAMYWPRMFFVTGAYHRYFSHRSYKTSRWFQFVLALGATFTAQKGPLWWAAH
                     HRVHHKLSDMPGDLHSVKQSGFWWSHMGWILSEDFKDTDLSRIKDFAKYPELRWLNKY
                     WIVPVLAAAVIAFLIGGFFGLVWAFAVPQVLCWHGTFTINSLSHVFGGRRYETEDDSR
                     NNLALAIVTMGEGWHNNHHHYQVAARQGFYWWEIDCTFYVLKALSLAGLVWDLHKVPE
                     HIKAPATEDAEDGVAALGDVA"
     gene            8490..9437
                     /locus_tag="VMZ53_02865"
     CDS             8490..9437
                     /locus_tag="VMZ53_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS27414.1"
                     /translation="MRFPPANIQLRRAQLILMLAALVPTIMLTVVGIMMLFVAHESLP
                     TLIFGVLILTLCTSGITGYILGSIFLGKGASLVRVQNDFVSAVSHELRTPVTSIRLLL
                     ESLATGRLDDADKQQVLDLLARETTRLELLVTRVLELSRLESSHVFAREPVDVAELVD
                     EAIAAFQANTLERPTPIMKKLEPHLVLLGDRPTLVRALLNLLTNAWKYTGEQKHISVE
                     AQAVGRTIEIIVRDNGRGIDADEQREIYEQFKRGRAAHDTGASGVGLGLAFVRTIVRG
                     QRGKLTLESRPGDTAFKIRMPRAKDPTGERAQVPERAPS"
     gene            9446..10129
                     /locus_tag="VMZ53_02870"
     CDS             9446..10129
                     /locus_tag="VMZ53_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS27415.1"
                     /translation="MLIVEDDEAIATGLALNLKLAGRGTSIARDGDDALRQALDEDFS
                     LILLDINLPKRNGLEVLSTLRAADNVVPVIVLSARDGEFDKVAALRLGADDYVTKPFA
                     LAELLARIDAVLRRTQAPAPPVATQHDDNLAFSDVAIDLGQRVATRGGAEVKLTHLEF
                     ELLVFFLRHPNQVFSRNQLFSHVWGQSAGSVRTVDNFVGQLRKRFEGDPEQPKHFITV
                     RGSGYRFNP"
     gene            10233..11312
                     /locus_tag="VMZ53_02875"
     CDS             10233..11312
                     /locus_tag="VMZ53_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27416.1"
                     /translation="MKRLLLLASASILPLSVGCYGGERYNTGECPAGETCSDLTPKGL
                     QFVGNSLVDDILLSGPRATAVGGTQVVALQYSRGDGVTVALDLPYTADDDGGNGVKVS
                     AQSGSQVTMLGAGSRSNYLRILDSDGLLMDRKELTGAALERIELTPTDFESIPNGSEL
                     AFAPGKRKLGVALYGDVQHSSGPVSERIVDTSMQLDLAGAQKTAWDTLEVANAQIGRS
                     TLTVTAGDKPALNIDFVVVDHADAVAVTEPAPSTIMPSGSQTVCFKATMADVTGPRYI
                     VGLTWTFNVDGQIKTQGDGVLTRNCISVTTTKTTGSVSVQASAGGQSSTVSLAVGAAR
                     TIAPVGTGMHTAAEAVSTDGDRAAL"
     gene            11524..11931
                     /locus_tag="VMZ53_02880"
     CDS             11524..11931
                     /locus_tag="VMZ53_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HUS27417.1"
                     /translation="MADTIFGKIIRGEIPCHRIYEDDKVLAFLDVNPVSKGHALVIPK
                     EPAETLDQLSDDSAAAIGRVLPRIARAVLAATGATAFNVLQNNKPLAHQSVFHVHFHI
                     IPKFDDGSGLGIEWNATKIDGGAELAKAISSKL"
     gene            11928..12986
                     /locus_tag="VMZ53_02885"
     CDS             11928..12986
                     /locus_tag="VMZ53_02885"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HUS27418.1"
                     /translation="MRALAVAALAGAVASSGCLYTRYVAQAAHGQFELLGKAKPIEKV
                     IADPDTPTRVAMLLAEIPEIKQYGRSYGLKIRKNYSTYSSLGRPAAVWFTGAADPVAF
                     KPLRWCFPIVGCFAGLGWFDEDEAVKFKLDLEAKGYDAIVRPASAYSTGGWFPDPVLS
                     TMLGGGDDALPELANVILHESVHATVLVNDEPFFNESLASYVADSLTDAWIQQRFGPG
                     SPEEVAWTLGQALHLPRVARMLAAYKALKAVYDSKRTRAEKLAEKAKIIDELVADLDI
                     RRRPNNASLTEVRVYNGGAAPLLVAHRKCGDLRSLIEAAKTLKRRDFAKPLEDDLTGV
                     GKLLAERCVQKSARKLDH"
     gene            13012..13800
                     /locus_tag="VMZ53_02890"
     CDS             13012..13800
                     /locus_tag="VMZ53_02890"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HUS27419.1"
                     /translation="MRALAVAIGLLAFAATADAKPRKQPKVAKVAKVEKSTKRAKKSA
                     KKAGKRSAHAAREKRFEVKGPIHGQSVGAPWSGRLRSATELPEGDGYVLRRPWRAYGT
                     KSMVESIYHVVGRVHEQFPDMHDLAIGDLSAKDGGRISEHNSHQSGRDVDLGLIYYKQ
                     PAHYPDSFIPATEENLDCEATYALVEELAKTGRIQMMFLDFKVQGMLYDWAKANDVDE
                     DHLRQLFQFPHGRGSSEGVVRHEPNHQDHIHVRFRCPAGDTACR"
     gene            13827..14639
                     /locus_tag="VMZ53_02895"
     CDS             13827..14639
                     /locus_tag="VMZ53_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27420.1"
                     /translation="MRTLVWVVALAACGDDGSGVGGDGGTKDANVPLNTGAILISSFR
                     SKQVGTEVSNTIVNASFSPQQTSTPPCTSQTVGPCIVSQCSGPFPMQTLASGGTVTVM
                     GVGTTATLTPGFGNMYQPVNTGASFAGGETITMQGTGADTPAFSFTLLAPARAAITTP
                     AKPSGTDALVIDRSHDLSVAWTGGGTSEIDFVFGSPGLVNMNCAFPASAGSATIPTAA
                     LQMLSAGSGSFSASTEVIKSVDVGAWRFYGEAFFTVVWSIDESNGSVTTTLQ"
     gene            14658..15605
                     /locus_tag="VMZ53_02900"
     CDS             14658..15605
                     /locus_tag="VMZ53_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HUS27421.1"
                     /translation="MGDAYRTSAFMCPVCEGATLREYKERLVCDQCQGMMLSTDDFAD
                     SIREIDGSQDPIAVTDTDATKRGCPHCSVPMKECKVTLGSLSVAGAMHCATHGIWMPR
                     KQMTSAFARASRRGGFAGMGATGGARSRFGGGGSGADASSIIANMPSAHSGMSGAMAG
                     IASAFGGGGPASGGLAISHWQSRRPRAHTIYVSAHKDQQLGCPACKKPLAYWGDRWRC
                     AHCPGLFVETAALVGMVSEMVNGPWELPPLAGKPGERVCSICESPMVVEVLEGVTVDR
                     CEHAHGVWFDEHELEQVLHHASTPHGGLGTWIKRLFGRG"
     gene            complement(15602..16765)
                     /locus_tag="VMZ53_02905"
     CDS             complement(15602..16765)
                     /locus_tag="VMZ53_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27422.1"
                     /translation="MRRLLPASFLLFAACNFPHGSLGTGDGGSTGDDDASDAAIDAMI
                     DAPDPSCYGRGAFYVCFMPAPTGDVTLQGNIDTTACTAETTGSTLVGATKMVSGVSVC
                     VIAAASFTMQGATVGTLGDKPLLLLATGNITIPTGVELDGRSAAFPLVGSPGDGPNAN
                     PSDCGNRPSGASSTTGGGGGAGGTFGTKGGNGGNGGGAMGGMAAAVPTPFVKLRGGCP
                     GGNGGGGNGSTGGAGGSGGGAIALFSHGTIQIDGTITVSGAGGEGGNASKGGAGGGGS
                     GGMVVLDGAAVMMGVAGKIIANGGGGGAGAGNSTSGDDGKDAIVLDAAAPGGVLSASG
                     ATPGGAGAFGSTAAGSPQPGANGGGGGGGGAGVIRVLSGQALPSTKVSPPPTP"
     gene            complement(16743..19235)
                     /locus_tag="VMZ53_02910"
     CDS             complement(16743..19235)
                     /locus_tag="VMZ53_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HUS27423.1"
                     /translation="MSNEMPAQLARRYVAWLKRRVFVMIGVYVAVLGIALYLIAFRLP
                     LFADFSYLLPQDVPAVRDLRKLEARTKSVDTMLVIIRAPSPQAQAAATKQMIDGITAL
                     KSPLVARVMGDDKASRDFLHSRRALLVDVADLEKARDALQHRIEQAKLKANPLYIDLG
                     SEEDEAQQAADQKQLDELREKRREAEARLTRPTNVSPDGLVAKIEVATAFRTTDAGRA
                     EQLLVSLGAIRAQVVASNPGVDIGFTGGAVTGLAEHRAITKGMMLSSAVTALLVALVL
                     ALYFRSATFLVLLVGTISIATAAAFGAAAITVGHLNAATAFLGAIIAGNGINYGIFLI
                     ARYLEERKRYEVDDAIAAAIAGTLRPTAVASLGAAIAYGSLAATSFKGFADFAVIGAI
                     GMMLCWVATYTLLPALMLRFGRHTRIYHGNPIVGSMLVRLFGFKRSGMVVVAAIALFS
                     VAGVVVVRYVAADPFEYNIKNLRSEGEDAVESRHWMETSDKNFGRGISGRTYIAADRL
                     DQVPMIVEALQSIDANKPRAEKTIGVIDALPAYVPNDQAERMKLLADIRDRIDEAAEE
                     LEDKDKQELLELRPPDDLKPFTVDDLPPTLRERFTEKDGRVGYLIAVRPSNDLDEWNG
                     KDLIRFATAVRELHLPDGETVTTSGSSVIFADIVTSIERDGPVVTAVALGGLIIMVVL
                     LVGRNRRAAAVMIGTIGGAVLLIAVCALFDLKVNFLDFVALPITLGLGIDYAINVAHR
                     HDAEDVPDPVTTLRTSGSAVLICSLTTMIGYGSLLVSENLAIRGFGTASLIGEVTTVL
                     TALVLVPALLAIGNRRRRGSGTLASGASASPS"
     gene            complement(19395..20075)
                     /locus_tag="VMZ53_02915"
     CDS             complement(19395..20075)
                     /locus_tag="VMZ53_02915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUS27424.1"
                     /translation="MRLVLLSLTVVLLASSAASAGPFLDGTLVGRLATLAPAPPDLSV
                     LTTEPVERTESSGYGWREDPIRKNQRFHHGADYRADPGTPVLAAGNGVVAFAGRQGGY
                     GNVIYVDHGNGVVTRYGHLRRIETKKGDVLTAGTRIGQVGSTGRATGPHLHFEVRLDG
                     RDVDPNTALAVAELSRESPELGHIASFALAPELQKMSRDMQDKKNRRRAEARPERDNA
                     PKRSQALW"
     gene            20275..20670
                     /locus_tag="VMZ53_02920"
     CDS             20275..20670
                     /locus_tag="VMZ53_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27425.1"
                     /translation="MQSAWWTMSSVIVGPFGTRNCPGGADECGFAWLGTNHDLFMRSA
                     VATRAGGYIAMFVYIMVAGAIAAKRVPSLLSKAGIVAILTASAAAITFFVKFPGSFGE
                     PSLGAGPILFVVGVVAGVIASVRVLRAQR"
     gene            complement(20633..21436)
                     /locus_tag="VMZ53_02925"
     CDS             complement(20633..21436)
                     /locus_tag="VMZ53_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HUS27426.1"
                     /translation="MNAAWILAAGGNEYTAQIALSGAIPAIIAMALVDRLDRKRPEPP
                     GTRRLAAFLGMLSIIPAIILEMLLSKKLGANIEPQMTYQGSAFHAFVIAAAVEEGCKA
                     ALIFMFIWHKPEFDERMDGIVYFSRAGLGFALVENIGYLLMQQSLSGQLTTWILRAVL
                     AVPGHAIWTGIIGAMAARRRFDKKGLGLLGGYLLAVAMHGLYDLCAFLQAPLSFEGYD
                     TLAMLMMPALVLITVLGFFILRDQAKTALRLDDADVALNAAHAARAQMR"
     gene            21499..22377
                     /locus_tag="VMZ53_02930"
     CDS             21499..22377
                     /locus_tag="VMZ53_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27427.1"
                     /translation="MLRVLAVLVLLVSVAPVAHAGRGTNLVKYLPDDTNVIVIADVSR
                     ARRSPIFKKAFEAARSKSDTLDNLASTLAVDKLVDTIAVGGNGAAQRAVVVLEGRVDK
                     LLAEAKKQATKEDKHGGVAFWTVPDGEVAVVDKKLVIASAGEMEHVIDRAQDKKAKGP
                     AAVRTILASATANSSVFGGAVLDADQKKEMAKEIGAEPQWAAFSFAMAQKLTIDAKVK
                     LADDASAEKVAKSINEKLGAPGEDGTNRSRIEGFVGKDFADSIVVDQDHSFARISATM
                     SADEFDKVLALVKMFM"
     gene            22460..24028
                     /locus_tag="VMZ53_02935"
     CDS             22460..24028
                     /locus_tag="VMZ53_02935"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27428.1"
                     /translation="MPEPSAPGMPQRVGRYEVITHLATGGMAQIYLARQSGLGAFERH
                     VVLKTILRERATDQRFVTMFLDEAKLAATLNHQNIAQVYEVDQADGAYFMAMEYVHGE
                     NARAILETTLRRGWTIPLELAVMIVSGAAAGLHHAHERRGKNGQPLNIVHRDVSPANI
                     MVGYDGSVKVLDFGIAKAEERATKTVGGTIKGKYGYMSPEQCKGKPIDRRSDIFALGI
                     CLYELTTLRRAFKGNDDFETMKRIVAGDVLLPSVAVPGYPRELEAIVLTAMANDPNAR
                     FQSGQEMIEALDAFAVRAKLTGSNTAMGRFMVQLFGSKKEPWIEAPSGSDRTQVTEPS
                     GEMDPDNDEKTTLIESKQQPEYGRAATATAPGTGMGPQVGRQSGEVQSLSASAAAADW
                     NHDSRAGRHSSPRPGFDQPRTPPNGYQLPNAAMTSTLAGTGPVPAIPGRIPSRAETEP
                     GSKPLVDDRLAWQQPQNPAMRDHRLPPPGATRLPTQYQDPNFKITSNRGWIILAILML
                     AGLGAGVLIAMQSS"
     gene            24050..>25192
                     /locus_tag="VMZ53_02940"
     CDS             24050..>25192
                     /locus_tag="VMZ53_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUS27429.1"
                     /translation="MATATTQNSERLQSYNPATGESIGSVPIMTPAEVDAAVARARVA
                     ATTWGTRSFKARAEELTAFRIALAEAADEIAELLHRENGKPLLEGYVEVMMALSHVKH
                     ASIVAEEAMRPRKVASGVLANFRATISYFPLGVIGVIGPWNYPLFTPMGSIAYALAAG
                     NAVIWKPSELTPLIALEIEKIAKKTFALPDLLQVVTGAGTTGAALAKSAVDKIAFTGS
                     AATGKRVMAAAAERLTPVLMELGGKDPMIVDTDADLEKAAEAAVFGGLTNSGQACVSV
                     ERVYVSEPVYDRFVDEVVKQVRELKVGGDDGHLGAMTSPAQVAIVKDHLEDAVKKGAK
                     VLTGGPDAISGSYIQPTVLTNVDHKMKVMSEETFGPVIPIQKVKSVD"
BASE COUNT         4472 a         8321 c         8184 g         4215 t
ORIGIN      
        1 ggcaaggccg tgtcggttca gatcggtatg aacctgacgg tcgtcggcag caaagacgcc
       61 gagcgctagc cgaagcgtaa gcgtaagcgt aggcgtagcc ggcgatgcgc aaccggagct
      121 cggactggtc gatacaccga ccatgttcca cttccgcagc ctcgacgtgt atcgctgtgc
      181 agtcgcgttt ctgcccctcg cctacggaat ggcaaaggag ctcgacagcg agatggccgg
      241 gcaacttcga cgggcggcac tatcgatcaa cctcaacatc gcggaaggga cagggcggtt
      301 cgatcgggac caacgcaggt tcctggtcac ggcccgcggc tcggcactgg aatgcgcggc
      361 cgtgctcgat gcgatgactg ctctcgagct tgccgatgag cggatcgcca ctggcgatgc
      421 ctttctcgtg agaatagtgt ccatgctaac gaagatgatc ggctgacgct tacgcttacg
      481 cttacgccta cgtctcggat caacctctgg gtttagtgta aaacgctgcg catgcacggt
      541 acgcgcatcg cgctcatcct ggcgggtgaa gttcccgtcg gcacgaaggt cacgatcgag
      601 ggctgggtcc ggacgcgccg cgactcgaaa gccggcctgt cgtttctcgc gattcacgac
      661 ggcagcagcc acgagccgct gcaagtcatc gctccgcagg agctgcccaa ttatcaggac
      721 gacgtcctga agatcacgac gggctgtgcg gttcgcgcga ccggcgagct cgtcgcatcg
      781 cagggcaagg ggcagtccgt cgaagtgcgc gccgacaaga tcgaggtcgt cggctgggtc
      841 gacgatccgg atacgtatcc gatgcagccg aagcggcaca cgatggagta tctgcgcgag
      901 gtcgcgcatc tgcggccgcg cacgaacgtc atcggtgcgg tcacgcgcgt acggcatgcg
      961 ctcgcgcagg cggtgcatcg gttctttcac gagcacggct tcttctggat ccacacgccg
     1021 atcatcacgg cgtcggacgc cgagggcgcc gggcagatgt tcaaggtgtc gacgctcgac
     1081 tacgcgaatc tgccgcgcac ggccgacggc aagatcgatt tcgacaagga cttcttcggt
     1141 cgcgaggcgc ggctgacggt ttcgggccag ctcaacgtcg agacgtactg catggcgctc
     1201 tcgcgcgtgt acacgtttgg gccgacgttc cgcgccgaga actcgaacac gcggcgccac
     1261 ctcgccgagt tctggatgat cgagcccgag atcgcgtttg ctgatctcaa cgacgacgcg
     1321 aacctcgccg aggagttcct caagtacatc ttccgcgcgc tgctgacgga gcgcaaagac
     1381 gacatggcgt tcttcgagaa gttcgtcgac aaggagtgcg taacgcgcct gtcgaagatg
     1441 atcgagtcga agttcgcgcg catggactac accgacgcga tcaaggagct cgagaagtcg
     1501 gggcagaagt tcgagttccc cgtgaagtgg ggcatggacc tgcagagcga gcacgagaag
     1561 tatctgaccg aggtcgtgat caagggcccg gtcgccgtcg tgaactaccc gaaggacatc
     1621 aaagcgttct acatgcgcat gaacgaagac gggaagaccg tcgctgcgat ggacgtgctc
     1681 gcgcccggca tcggcgagat catcggcggc agccagcgcg aagagcgact cgagatgctc
     1741 gacaagcgca tcaccgagat gggcctgcac gtgaaggact actggtggta ccgcgatctc
     1801 cgtcggtacg gcaccgttcc gcacgcgggc ttcggcctcg gcttcgagcg cgcgatcatc
     1861 tatgcgacgg gcatcgagaa catccgcgat gtgatcccgt tcccgcgcgc tccgcgtcag
     1921 gcggaatttt gaaagacgct gtcctcgccg cgctcgccga ctatgcggct ggcaagacgg
     1981 gcgcgacgcg tgcgattgca ggcgcgatcg cgtcgcactt tctcgcggtg acgacagcac
     2041 gcagcctcgc gctcgtcggc ggcgatgtga gcgtgcagtc actgctggcg catcgcacgt
     2101 ggttcgatcc gaaggacatc cgttgcacgt cgggctccgt ggcagccgcc gtcggcggtc
     2161 gtcaggtccc gctcgccgaa gcgctcgccg ctgacattgt ttgcgtccac gagccgatcg
     2221 cgatcaccgg cgtgcagcta cgacggggaa cgcacatcaa cgcgctcgcc gccgtcacgc
     2281 tcgattcgga gcttcgctcc gtggcgacgc tcgtcgatga agcgggcctg cacaagctcg
     2341 ccgccggcat catcgacggt cgccagctcg acgagctgac cgtgttcgtt tacggcgccg
     2401 tgccgtagac gaggacgctc ttgaccgaga gcgtgcttcc cgtcgtcgtg ctgatcctga
     2461 gcttaccgtt cgtgaacacg acgggcgcgg cgtcgctcag catgccgttc gccgtgatgt
     2521 agcagaacgt attcgatccc tgccggtaga ggttcactgc gagcaggccc gacggtgctc
     2581 caagctggcc accgacgccg ccgcttgcat ccatctgcac gcacatcgag ccagtcgttg
     2641 gcgacgttcc acacgtgcga gtgcggatgt gacagaccgc gttcggcgcg tcgatccgcg
     2701 cttcgtcatt gatgtccaca ccggtgaggc ccgcggtgag gatctcgatc ttcgtcggca
     2761 ggaacatggc cttcgtcatg atcgaaccgt tcgcgatcgt cgcgacgcca ccgccggcag
     2821 gatcctctgc gacggagccc gcgatatcga atctgtcgag atccgtcccg ccgaatgcgg
     2881 cgaagaacag aatcgcgtcc cgcccgccgg cgccgcggtt ctggtcgcag ttgtcccaga
     2941 cgccgtcgtc gtcggcatcg gcatcgtcgg ggtccatgac cgtcgggcag ccgtcgcaac
     3001 gatcggggaa gccgtcgctg tcttcgtcgt gctcgttcgg cgcgcatgca cccggtccat
     3061 cgggcattgc atcgggaccg cgtgcgtcgg catcaccgcc gccaccgtcc tcacgcacgg
     3121 ggcctacgtg atccacgctg aagacgctat cgcagccggc gagcgcgaac gcgaacgcga
     3181 ggagccgcca gcgagacatc aaggacccga tccgtagacg accatgttga ggatcttcac
     3241 gccgctgatc gcggtcgcgg tcaccgtgat cggtgcttgc gagagttgcg cggtgctccc
     3301 gatgcctgtc gtgctcgacg ccgtgaccgc gaacaccgtc gacgtaccgc ccgcatttct
     3361 cgagagctcg atccgagtga cgttgctcgc cttcgtctgg agtatcccga aaccgctgcc
     3421 ggtctgcgtg agaaagcagg tctgcgtcag cgtggtctgg tcgcagcctg ccgcgtggac
     3481 ctcggcggtg atcgacggct ggctcacggc gacgattgcg ctgtagccgc cgccttcgac
     3541 acccgtgatg ttgagcacga tcctcgtcgg cgcgagcgag acctttgtcg tcatcgtcgc
     3601 ggcaagcgcc ccggttccgt tcacgtagac aaggctgctc gggaacatgt tctcggtgtt
     3661 cgtcgagtcg aacttggcgc cgagctcgag aggatcggag aacagcgcgg cgaacaggat
     3721 cttgtccttc tgaccggttc ccagatccgg atcgcatgcg tcgggtaggc cgtcgaggtc
     3781 tgcatctgcc tcgctgccgg cgaccgtcgg acacaggtcg cagccgtcca tccggccgtc
     3841 cgcatcctcg tcgaaggtgc cgcagtcggc cgcgtccgcc atcaatgggt cgccgtcgcg
     3901 gacagcggcg tcgtcgtctc ccatactgcc gtcgctgccg ccgccaccac ctggcggcac
     3961 gtggtcgagg ttgaacaagc tgtcgcaacc tccgagcaac aacagcgcga ggacgcaggt
     4021 acgcatcacc agcccgctgt gcggagccaa cgttcgagcc tcgcggcgta gacgagatcg
     4081 agcagcgtga tcgccttctt gtcgtgcgtg tggatcgtga gcgtcatggc cgcgtactcg
     4141 agcacgatcg tcggatgatg atccatctcg tccgcgagcg tcgcagcatg cgcggccgcc
     4201 gcgccgtact tcgccatcgg tgcaccctcg agcctcagct tgaggttctc gccatcgatc
     4261 gaccagcgcg cgccgagagc cgcgacttcc ttgcgcagct gttcctccga gagaacagag
     4321 cgactcacgg tgttactcgc gagcgcgtcg aggttgtcag ccatcgcctc aagctatcat
     4381 ctcgcgcatg gagatgtacg acctcacggt tccccagctg ggtctagcgc tgcggaacat
     4441 cgacggctgg atgatccgtg cggtgaagca cgccgacact aagcaagtcc cgcatgacca
     4501 gctgctgtcg gcgcggcttg ctcccgatca gtatccattc gttcgccagg ttcagaccgc
     4561 atgcgacaac gcgaagctca tcgccgcgcg actcgccggc aagcaggcgc ccgggcatcc
     4621 cgataccgag acgacgttcg agcagctgcg cgcacgcatt gcggtcgtcg tcgagtacct
     4681 gaccacgttc acccgcgagg atttcgcggc ggccgcggag cgaaagatcg tgctgccgtg
     4741 gatgacgaac ggccagcaca tggccgcgac cgactacgtc acgcagttcg ggctcccgaa
     4801 cttctacttt cacgtcgtga ccgcgtacgc gatcctgcgg aaccagggcg tgcccctcgg
     4861 gaaaaacgac ttcctcggcc agatcacgat tcgcacgtaa ggcgcggtat cctgcggcct
     4921 cgcgatgacg agtgggatcg acgccgggat ccgggtcggg aagtacgagg tcggccgcaa
     4981 gctcgggcaa ggcggcttcg gcatcctgta cgtcgcgcgc gatcacgagc tcggccgcga
     5041 aatcgcgatc aagttcctgc gccccgagca cgcgttcaag ccgacggtcg tgcagcgctt
     5101 cctccaagag gctcgcgccg ccgctcgcat caatcatccg ggaatcgtca ccgtcttcga
     5161 gagcggcctc gtcgccggca ccaacacgcg cgcggacggc accgtcttca tcgcgatgga
     5221 gctgctcgcc ggcgacacac tcgcgcagaa gatccgcggc ctcggcaggc taccgacgac
     5281 gctggcaatc aactacgcgc ggcagctcgc atcggcgctc ggcgccgcac atcaagcggg
     5341 catcgtgcat cgcgatctca aaccgccgaa cgtgttcgtg gtcccagatg cggcggtcgt
     5401 cggtggagaa cggctcaaag tcctcgactt cgggatcgcg aagctcgccg acgatttcgg
     5461 cagcgggatg cagacgcact cgatgctgat gctcggcaca ccgatgtaca tgtcgcccga
     5521 gcagtgcaag tcgagcgcga aggtcgacgc gcgcagcgac atctattcgc tcggctgcat
     5581 cttgttcgag atgctctcgg gcaagccgcc gttcgacggc gattcgggag agctcatcgc
     5641 gaagcatcag ctcgtgccgc cgccgacgat gcgctcggtc atcagtgaca taccggtgcc
     5701 gctcgatcga ctcatcgcat cgatgctcgg gaagtcgccc gccgatcgcc cgcagacgat
     5761 gggcctcgtc gaggacgcgc tcgcagagat cgactcacgc gcggtcgagc ggccgtcgat
     5821 tgcgggcgag gacctgcccg ccatggaggc atacgccccg acgacgatcg aaccgtcggc
     5881 gatgcgcaaa cgcctgccgc tcgtcgccgg agctgccggt gccgccgcgc tcggcatcat
     5941 ggcggggttg ctcgcgatgt gcggcgacaa gaagccggcg gcccaagcga acgtcgcgcg
     6001 cgatgcgagc atcatcgccg aagcggcacc cgacgcggtc gagctgacgc ctgacgcgat
     6061 ggagctgacc gtaacgcccg atgcagcggt cgccgaggaa ccagtgccgc ccgtggatca
     6121 catcgatccg agcatcacgc ccgcgcgagt aacgaccaaa tcgccgacga agccggcgac
     6181 gaagacaccg gcgaaggcga agaccgcgaa gaccactgac gtgaagccac cggtcaactg
     6241 cgatgccgat gggctcaaag acaagggcat ggagaacgtc aacatgggcc agcacgcggc
     6301 agcgctcgcg cagttcgaag cgtcgctgcg ctgcaagccc gacagctacg tgctcgcgct
     6361 cgcgttcatg gccgcgtgta acgcgcagaa cgtggcgaag gcgaagtcct actatctaaa
     6421 gatttcgccg ccagagcgcg aacgctacaa agtgatgtgc gtccgcaatc acatcaatcc
     6481 tggcgactga tcaccacgtt ccctgcgtct gcgcgatgaa cgtgtacgga tagccgagat
     6541 cgatcgcact cgccttgtcg agagtctcga cgtgcttcgc cggaagctcg agctcggcac
     6601 cggcgaggtt cgcctcgagc tgctcgatcg tgcgcgcgcc gaagatcacc gacgtgacct
     6661 gcggcttcgc gagcaaccac gcgaggctta cggcggccgg cgtcgagccg acctctgcag
     6721 cgacgctctt cactgcgtcg agcaccttcc agttgcggtc gctgtcgtaa cgcgccatgc
     6781 gatcgggctt ggcgcccagg cgtgtcgtcg catccgccgc tttgccacgc tcgaacttcc
     6841 cggtgagaaa tccgcccgcg agcggcgacc acggcaggat gccgaggcct tcttcgcggc
     6901 acagcggcac gtgctcgcgc tcgaggtcgc ggacgatcaa gctgtactgc gcctgcagcg
     6961 tcacgaagct cgcccagcga ttcgtgcgtg atagccagag gctgttcatc aagcgatacg
     7021 ccgcgtagtt cgagcagccg gtgtacagca ccttgccggc gctcacgaga tcatcgagtg
     7081 cgcgaagcgt ctcctcttcg ggcaccgtgg tgtcctgcat gtgaatctga tagaggtcga
     7141 tgcgatcggt cttgagtcgc cgcagcgagt cctcgcagca cttcacgatg cgataccgcg
     7201 atgcgccgct gcggtttgcg ccctcgccca tcgtgaagcg gaacttcgtc gcgagcacga
     7261 ccttgtcgcg cgtgccgcga gcttcgaacc agttgccgag cacgcgctct gagagaccgt
     7321 cttggccgta gacatccgcg gtgtcgatga agttaatgcc cgcggcaagt gcgcgatcga
     7381 gcacggcgtg cgcggttgcc tcgtcagacc cgaccttgtg catgaacgat ttctcgtcgg
     7441 cctcgccgaa cgtcatcgct ccgaggcaaa gactcgatac cttcacgccg gcggcgccta
     7501 ggttgcggta tttcatgagc gcgagcctac tctgacggaa tcatgaccgt cgatggcacg
     7561 cgcactgcct attctggcag tagccgatgt ctgtgaacgc agtcgcagcc gcgcagtcga
     7621 aaggctggcg ccacaattac ttcaacgcgc tctcgattcc gttttggggc gtgcacatcc
     7681 tcgcgatcat cggcatcgcg atcacgggag tgtcgtggct cggcctcgcg ctcgtcgccg
     7741 ccatgtactg gccgcgcatg ttcttcgtga ccggcgcgta tcaccgctac ttctcccatc
     7801 gcagctacaa gacgagccgg tggtttcagt tcgtgctcgc gctaggcgct acgttcaccg
     7861 cgcagaaagg tccgctgtgg tgggccgcgc atcatcgcgt gcatcacaag ctcagcgaca
     7921 tgcccggcga tctccactcg gtgaagcaga gcggcttctg gtggtcgcac atgggctgga
     7981 tcctgtcgga ggacttcaag gacacggacc tgtcgcgcat caaggacttc gcgaaatatc
     8041 ccgagctgcg ctggctcaac aagtactgga tcgttcccgt gctcgccgca gcggtgatcg
     8101 cattcttgat cggtggcttc ttcggtctcg tgtgggcgtt cgcggttcca caagttctct
     8161 gctggcacgg cacgttcacg atcaactcgc tctcgcacgt gtttggcggg cgtcgctacg
     8221 agaccgagga cgactcgcgt aacaacctcg cgctcgcgat cgtcacgatg ggcgaaggct
     8281 ggcacaacaa ccaccaccac taccaagtgg ccgcgcgcca aggcttctac tggtgggaga
     8341 tcgattgcac gttctacgtg ctcaaggcgc tctcgcttgc cggcctcgtc tgggacctcc
     8401 acaaggttcc cgagcacatc aaggcgccgg cgaccgagga cgccgaagac ggagtcgcag
     8461 cgctcggcga cgtggcatag tccactcccg tgcggtttcc acccgcaaat atccagctcc
     8521 ggcgcgctca gctgatcctg atgctcgctg cgctggtgcc gacgatcatg ctgaccgtcg
     8581 tcggcatcat gatgctgttc gtcgcgcacg agtcgctgcc gacgctgatc ttcggcgtac
     8641 tgatcctgac gctctgcacg tcgggcatca cgggctacat ccttggctcg atctttctcg
     8701 gcaaaggcgc gtcgctcgtt cgcgtgcaaa acgacttcgt ctcggcggtc tcgcacgagc
     8761 tccgcacgcc ggtgacgtcg atccggctct tgctcgaatc gctcgcgacg ggacgcctcg
     8821 acgacgctga taaacagcag gtgctcgatc tgctcgctcg cgagaccacg cgcctcgagc
     8881 tgctcgtcac gcgcgtcctc gagctgtcgc gtctcgagtc cagccacgtg ttcgcgcgcg
     8941 agcccgtcga cgtcgccgag ctcgtcgatg aagcgatcgc ggccttccag gcgaacacac
     9001 tcgagcggcc gacaccgatc atgaagaagc tcgagccgca cctcgtcctc ctcggtgatc
     9061 gtccaacgct cgtgcgcgcg ctgctcaatc tgttgacgaa tgcgtggaag tacacgggcg
     9121 agcagaagca catcagcgtc gaagcgcagg cggtaggccg caccatcgag atcatcgtgc
     9181 gcgacaacgg ccgcggcatc gatgccgacg agcagcgaga gatctacgag cagttcaagc
     9241 gcggtcgcgc cgcgcacgac accggcgcat ctggcgtggg cctcggcctc gcgttcgtgc
     9301 gcacgatcgt ccgcggccag cgcggcaagc tcacgctcga atcgcggccg ggtgacaccg
     9361 cattcaagat ccgcatgcca cgcgcgaagg acccgaccgg tgagcgcgca caggtgcccg
     9421 agcgagcgcc gtcatgactc accgcgtgct gatcgtcgag gatgacgaag ccatcgccac
     9481 cggcctcgcg ctcaacctca agctcgccgg gcgcggcaca tctatcgcgc gcgatggcga
     9541 cgacgcgctt cgccaggcac tggacgagga cttctcgctc atcctgctcg acatcaatct
     9601 gccgaagcgc aacggcctcg aggtgctctc gaccctccgc gcggccgaca acgtcgtgcc
     9661 tgtcatcgtg ctctccgcac gcgacggcga gttcgacaag gtcgctgcgc tgcgcctcgg
     9721 tgccgacgac tacgtcacca agccattcgc tctcgccgag ctgctcgcgc gcatcgacgc
     9781 ggtgctccgc cgcacgcagg ctcccgcacc tccggttgcg acccagcacg acgacaacct
     9841 cgcgttcagt gatgtcgcga tcgacctagg ccagcgtgtc gcaacgcgcg gcggcgccga
     9901 ggtgaagctg acgcatctcg agttcgagct gctcgtgttc ttcctgcgcc atccaaacca
     9961 ggtgttctcg cgcaatcagc tgttcagcca cgtgtggggg caatctgccg ggtccgtccg
    10021 cacggtcgac aacttcgtcg gtcagctgcg caagcgcttc gagggcgacc ccgagcagcc
    10081 caagcacttc atcaccgtcc gcggatccgg ctaccggttc aatccctgac attttcgtcc
    10141 ggctcgtctg gcaaaacgta ctctcgaggc cgccaacgtg gcgtaacctc atggtccaac
    10201 cactggcacg accggtgctg aaatccacat ccatgaagcg cctactcctc ctagcctctg
    10261 cttcgattct tcccctcagc gtcggctgct acggcggcga gcgatacaac accggcgaat
    10321 gtcccgcggg tgagacgtgc tcggatctga cgccgaaggg cctgcagttc gtcggcaact
    10381 cgctcgtcga cgacatcctc ctctcgggcc ctcgcgccac cgcggtcggc ggcacgcaag
    10441 tcgtcgcgct gcagtacagc cgcggcgacg gcgtcaccgt cgcgctcgac ctgccgtaca
    10501 ccgccgatga cgatggcggc aacggcgtaa aagtctcggc gcagagcggc tcgcaggtca
    10561 cgatgctcgg cgccggaagc cgcagcaact acctccgcat tctcgattcg gacggcctcc
    10621 tcatggaccg caaggagctg acgggcgccg cgctcgagcg catcgagctg acgccgacgg
    10681 acttcgagtc gatcccgaac ggctcggagc tcgcgtttgc accgggcaag cgcaagctcg
    10741 gcgtcgcgct ctacggcgac gtgcagcact cgagtggccc ggtctccgag cgcatcgtcg
    10801 acacgagcat gcagctcgat ctcgccggcg cgcagaagac ggcatgggac acgctcgagg
    10861 tcgcgaacgc gcagatcggt cgctcgacgc tgacggtgac cgcgggcgac aagccggcgc
    10921 tcaacatcga cttcgtcgtg gtcgatcatg cggacgcagt cgcggtcacg gagcctgcac
    10981 cgagcacgat catgccgagc ggctcgcaga cggtctgctt caaggcaacg atggcggacg
    11041 tcaccggtcc ccgctacatc gtgggcctca cgtggacgtt caacgtcgac ggccagatca
    11101 agacgcaggg cgacggcgtg ctgacgcgca actgcatcag cgtgacgacg acgaagacga
    11161 cgggctcggt ctccgtccag gcgtcagccg gcggccaatc gtcgacggtc tcgctcgcgg
    11221 tcggcgcggc gcgcaccatc gctcccgtcg gtacgggcat gcacacggcc gccgaggcgg
    11281 tgtcgaccga cggcgatcgc gcagcgctct aaccgcgtaa gcgtaagcgt aggcggaggg
    11341 cggcgcatcg agcgtcgcct tttcgctttc ggccgcgaag agcaacgtcg cgtatcgcct
    11401 gctgcgccgc ggttcgagcg atatcgctca tcgccgggga gcgtcgctcg catcgtccag
    11461 ccgcagccgc ttacgcttac gcagacgctt acgtgcacgt gaccactgcg ataccctcgc
    11521 cgggtggctg acaccatctt cggcaagatc atccgcggcg agattccctg ccaccggatc
    11581 tacgaggacg acaaagtcct cgcatttctc gacgtgaacc ccgtctcgaa gggtcatgcg
    11641 ctggtgattc ccaaggagcc ggccgagacc ctcgatcagc tctccgacga ttcggccgcc
    11701 gcgatcgggc gggtgctgcc gcggatcgcg cgcgcggtgc tcgcggcgac cggcgcgacg
    11761 gcgttcaacg tgctgcagaa caacaagccg ctcgcgcatc agtcggtctt tcacgtccac
    11821 ttccacatca tcccgaagtt cgatgacggg tcgggcctcg ggatcgagtg gaacgcgacg
    11881 aagatcgacg gtggcgcgga gctcgcgaag gcgatctcga gcaagctgtg agagcactcg
    11941 ctgttgccgc gctcgccggc gctgtcgcgt cgagcggctg cctctatacg aggtacgtcg
    12001 cgcaggcggc gcacggccag ttcgagctgc tcggcaaggc gaagcccatc gagaaggtga
    12061 tcgcggatcc cgatacgccg acgcgcgtcg cgatgctgct cgcggagatt cccgagatca
    12121 agcaatacgg ccgcagctac ggcctgaaga tccgcaagaa ctactcgacg tactcgtcgc
    12181 tcggccgccc cgcggcggtg tggttcaccg gcgccgctga tcccgtcgcg ttcaagccgc
    12241 tgcgctggtg tttcccgatc gtcggctgct tcgccgggct cgggtggttc gacgaggacg
    12301 aggcggtgaa gttcaagctc gacctcgagg cgaagggcta cgacgcgatc gtgcggccgg
    12361 cctccgcgta ctcgaccggc ggctggttcc cggacccggt gctctcgacg atgctcggcg
    12421 gcggcgacga cgcgctgccc gagctcgcga acgtcatcct ccacgagtcc gtccacgcga
    12481 cggtgctcgt gaatgacgag ccgttcttca acgagagcct cgcttcgtat gtggcggact
    12541 cgctcaccga cgcatggatc cagcaacggt tcgggccagg ctcgcccgag gaagttgcct
    12601 ggacactcgg tcaggccctc cacctgccac gcgtcgctcg catgcttgcg gcctacaagg
    12661 cgctcaaagc cgtctacgac agcaagcgca cacgagccga gaagctcgcg gagaaggcga
    12721 agatcatcga cgagctcgtc gccgacctcg acatccgacg ccggccgaac aacgcctcgc
    12781 ttactgaagt gcgcgtctac aacggcggcg ccgcgccact cttagttgca caccggaagt
    12841 gcggcgacct gcgctcgctc atcgaagcgg cgaagacact caagaggcgg gacttcgcga
    12901 agccgctcga agacgacctc accggagtcg gcaagctgct cgctgaacgc tgcgtccaaa
    12961 agtccgcgcg aaaactggat cactgattca agagtccgca ggattgcgag gatgagggcg
    13021 ctcgctgttg ccatcggttt gctcgcattc gccgctactg ccgatgcgaa gccgaggaaa
    13081 cagcccaagg tcgcaaaggt cgcaaaggtc gagaagtcga cgaagcgcgc gaagaagtcc
    13141 gccaagaaag cgggcaagcg cagcgcgcat gcggctcgcg agaaacgctt tgaggtcaaa
    13201 ggacccatcc acgggcaatc ggtcggcgcg ccttggtcgg gccggcttcg ctcggcaacc
    13261 gagctccccg aaggcgacgg ctacgtgctg cgccggccgt ggcgcgcgta cggcacgaag
    13321 tcgatggtcg agtcgatcta tcacgtggtc ggccgcgtcc acgagcagtt cccggacatg
    13381 cacgacctcg cgatcggcga cctctccgcc aaggacggcg gccgcatcag cgagcacaac
    13441 tcgcatcagt cgggccgcga cgtcgacctc ggtctcatct attacaagca gccggcgcac
    13501 taccccgata gcttcatccc ggcgaccgag gagaacctcg attgcgaagc gacgtatgcg
    13561 ctcgtcgagg agctcgcgaa gaccggccgc atccagatga tgttcctcga cttcaaggtg
    13621 caggggatgc tgtacgactg ggcgaaggcg aacgacgtcg acgaggatca cctgcgccag
    13681 ctgttccagt tcccgcacgg gcgcggctcg agcgaaggtg tcgtgcggca cgagccgaat
    13741 caccaggacc acatccacgt gcggttccgg tgcccggccg gcgacactgc ctgccgctga
    13801 gctcccggct gtgttacgag cgatagatgc gaacgctcgt gtgggtggtg gcgctcgccg
    13861 cgtgcggcga cgatggcagc ggcgtcggcg gtgacggcgg cacgaaggac gccaacgttc
    13921 cgctgaacac cggcgcgatc ctgatctcgt catttcgctc gaagcaggtc ggcaccgaag
    13981 tatcgaacac gatcgtgaat gcgtcgttca gcccgcagca aaccagcacg ccgccgtgca
    14041 cgtcgcagac cgtcggtccg tgcatcgtgt cgcagtgctc gggcccgttt ccgatgcaga
    14101 cgctcgcgag tggcgggacc gtcaccgtga tgggcgtcgg cacgaccgcg acgctgacgc
    14161 ctggctttgg caacatgtat caaccggtga ataccggcgc gagcttcgcg ggtggcgaga
    14221 ccatcaccat gcagggcacg ggtgcggaca cgccggcgtt ctcgtttacg ttgctggcgc
    14281 cggcgcgtgc tgcgatcacg acgcccgcaa agccgagcgg cacggacgcg ctcgtcatcg
    14341 atcgctcaca cgacctcagc gtcgcctgga ccggcggcgg aacgagcgag atcgatttcg
    14401 tcttcggttc gcccggcctg gtcaacatga actgcgcgtt tcccgcgagc gcgggcagcg
    14461 cgaccattcc aaccgccgcg ctgcagatgc tgtccgctgg cagcggctcg ttcagcgcgt
    14521 cgacggaagt catcaagagc gtcgacgtcg gcgcctggcg gttctacggc gaagcgttct
    14581 tcaccgtggt gtggagcatc gacgagtcga atggctccgt gacgacgacg ctgcagtaac
    14641 actgctacgc ttcacgtatg ggtgacgcgt atcgaacgtc ggcgttcatg tgtccggtgt
    14701 gtgagggcgc gacactgcgc gagtacaaag agcgcctcgt ctgcgatcag tgtcagggga
    14761 tgatgctctc gacggatgac ttcgcggatt cgatccgcga gatcgacggc tcacaggatc
    14821 cgatcgccgt caccgacacg gacgcgacga agcgaggctg cccgcactgc agcgtcccga
    14881 tgaaggagtg caaggtgacg cttggctcgc tctccgtcgc cggcgcgatg cattgcgcga
    14941 cccacggcat ctggatgccg cgcaagcaga tgacgtcggc gttcgctcgc gcgagccggc
    15001 gcggcgggtt cgcgggaatg ggcgcgacgg gcggcgcacg gagccgattc ggcggcggcg
    15061 gtagtggcgc cgacgcgtcg tcgatcatcg cgaacatgcc gtcggcacac agcggcatgt
    15121 caggcgcgat ggccgggatc gcgagtgcgt tcggcggcgg tggtcccgcg tcgggcgggc
    15181 tcgcgatcag tcactggcaa tctcgccggc cacgcgcaca cacgatctac gtcagcgcac
    15241 acaaagatca gcagctcggg tgccctgcgt gcaagaagcc gctcgcgtat tggggcgacc
    15301 gttggcgctg cgcgcactgc cctggcctct tcgtcgagac cgccgcgctc gtcggtatgg
    15361 tgagcgagat ggtcaacggt ccgtgggagc tgcctccgct cgcgggcaaa ccgggcgaac
    15421 gcgtgtgctc gatctgcgag tcaccgatgg tcgtcgaggt gctcgaaggc gtgacggtcg
    15481 atcgctgcga gcacgctcac ggcgtgtggt tcgacgagca cgagctcgaa caggtgctgc
    15541 atcacgcgag cacgccccac ggtggtctcg ggacgtggat caagcgcttg tttggccgcg
    15601 gctaaggcgt cggcggcggc gagactttcg tcgacggcag cgcctgacca gacagcacgc
    15661 gaatcacgcc ggcgccgccg ccaccaccac cgccgccgtt cgctcctggc tgaggcgacc
    15721 cagccgccgt cgagccgaac gctcctgcac cgccgggtgt cgcgccactc gcgctgagca
    15781 cgccacccgg tgcggccgcg tcgaggacga tcgcgtcctt gccgtcgtcg cccgacgtcg
    15841 agttcccagc gcccgcgccg ccaccaccgc cgttcgcgat gatcttgccg gcgacgccca
    15901 tcatgacagc cgctccgtcg aggacgacca tgccaccgga gccgccgccg cccgcgccac
    15961 ccttcgatgc gttgccgcct tcgccgccgg cgccggagac ggtgatcgtg ccgtcgatct
    16021 gaatcgtgcc gtgcgagaac aatgcgatcg caccgccgcc ggagccgccc gcgccgcccg
    16081 tcgagccgtt gccgcctccg ccgttgccac cggggcaacc gccgcgcagc ttcacgaacg
    16141 gagtcggcac cgccgctgcc atgcctccca tcgcgccgcc gccattgccg ccgttaccgc
    16201 ctttcgtgcc gaacgtaccg cctgccccgc cgccaccgcc ggtggtgctc gacgcaccgg
    16261 acgggcgatt gccgcagtcg ctcgggttcg cgttcggacc gtcgccggga gagccgacga
    16321 gcgggaacgc agcgctgcgt ccatcgagct cgacgcccgt cgggatcgtg atgttgccgg
    16381 tcgcgagcag cagcagcggc ttgtcgccga gcgtgccgac ggttgcacct tgcatcgtga
    16441 acgacgcggc agcgatcacg cagaccgata cgccgctgac catcttcgtc gcaccgacca
    16501 gcgtggagcc cgtcgtttcc gcggtgcacg cggtcgtatc gatgttgccc tgcagcgtga
    16561 cgtcaccggt cggcgcgggc atgaagcaga cgtagaacgc gccacggccg tagcacgacg
    16621 gatccggcgc atcgatcatg gcgtcgatgg cggcgtcgga tgcatcgtca tcgccagtgc
    16681 tgcctccatc tcctgtcccg agcgagccgt gcggaaagtt gcacgccgcg aacagcaaga
    16741 agctagctgg gagaagccga cgcaccggac gccagtgtac cggatcccct ccgtcggcga
    16801 ttaccgattg cgagtagtgc aggaactagc acaagcgcgg tgagcaccgt cgtgacttca
    16861 ccaatgaggg aggcggtgcc aaagccgcgg atcgcgaggt tttcgctcac gaggagggag
    16921 ccgtagccga tcatcgtcgt cagcgagcag atgagcaccg cgctgccgct cgtccgcaac
    16981 gtcgtcaccg ggtcgggaac gtcttcagca tcatgacgat gcgcgacgtt gatcgcgtag
    17041 tcgatgccga ggccgagcgt gatcggcagc gcaacgaaat cgaggaagtt caccttgaga
    17101 tcgaacaacg cgcacaccgc gatcagcagc acggcgccgc cgatcgttcc gatcatgacc
    17161 gccgccgcgc gccgatttcg accgacgagc aggacgacca tgatgatgag gccgccgagc
    17221 gccaccgcag tgacgacagg cccgtcgcgc tcgatcgagg tcacgatgtc cgcaaagatc
    17281 acgctcgagc ccgatgtcgt caccgtctcg ccatccggca ggtgcagctc gcgaacggcc
    17341 gttgcgaacc ggatcaggtc cttgccgttc cactcgtcta gatcgttcga cggccgcacc
    17401 gcgatcagat agccgacgcg cccgtccttc tcggtgaagc gctcgcgcag cgtcggcggc
    17461 aaatcgtcga cggtgaacgg cttcagatcg tcgggcggcc tcagctcgag cagctcctgc
    17521 ttgtctttgt cctcgagctc ttcagcggct tcatcgatcc ggtcgcggat gtcggcgagc
    17581 agcttcatgc gctcggcctg atcgttcggc acgtaggcgg gcaatgcatc gatgacgccg
    17641 atcgtcttct cggcgcgcgg cttgttcgca tcgatgctct gcagtgcctc gacgatcatc
    17701 ggcacttgat cgaggcgatc ggccgcgatg tacgtacgtc cggagatgcc gcgcccgaag
    17761 ttcttgtccg acgtctccat ccaatgccgc gactcgacag cgtcttcgcc ctcggagcgg
    17821 agattcttga tgttgtactc gaatggatcg gccgcgacgt agcgcacgac gacgacgccc
    17881 gcgaccgaga acagcgcgat cgccgcaacg acgaccatgc ccgagcgctt gaagccgaac
    17941 aggcggacga gcatgctgcc gacgatcgga ttgccgtgat agatgcgcgt gtgccggcca
    18001 aagcgcagca tcagcgccgg cagtagcgtg tacgtcgcga cccagcacag catcatgccg
    18061 atggcgccga tgaccgcgaa gtcggcaaag cctttgaagc tcgtcgctgc cagcgagccg
    18121 tatgcgatcg cagcgccgag cgaagcgacc gccgtcggcc gcagcgtgcc cgcaatggct
    18181 gcggcaatcg cgtcgtcgac ctcgtagcgt tttcgctcct cgagatatcg cgcgatcagg
    18241 aagatgccgt agttgatccc gttgccggcg atgatcgcgc cgaggaacgc cgtcgctgcg
    18301 ttgaggtgac cgacggtgat ggccgccgct ccgaatgctg ccgcggtcgc aatcgagatc
    18361 gtgccgacga ggaggaccaa gaaggtcgcg ctgcggaagt agagcgcgag cacgagcgcg
    18421 acgagcaacg ccgtcaccgc gctcgacagc atcatcccct tcgtgatcgc tcgatgctct
    18481 gcgagccccg tgaccgcacc gcccgtgaag ccgatgtcga cgccgggatt gctcgcgacc
    18541 acctgcgcgc ggatcgcgcc gagtgacacg agcagttgct ccgcgcgccc cgcgtcggtc
    18601 gtgcgaaacg cagttgcgac ctcgatcttc gcaaccaagc cgtcgggcga gacgttcgtc
    18661 ggtctcgtca acctggcttc cgcctctctc cgcttctcgc gaagctcgtc gagctgcttc
    18721 tgatccgctg cctgctgcgc ttcgtcctct tcgctgccga ggtcgatgta gagcgggttc
    18781 gccttcagct tcgcttgctc gatccggtgc tgcagcgcgt cgcgcgcctt ctccagatca
    18841 gcgacgtcga cgagcaacgc gcgtcgcgag tgcaggaagt cgcgactcgc cttgtcgtcg
    18901 cccatcacgc gcgcgacgag cggcgacttc agtgcggtga tgccatcgat catctgcttt
    18961 gtggcagccg cctgcgcttg cgggctcggc gcgcgaatga tcacgagcat cgtatcgacg
    19021 gacttcgtgc gcgcttcgag cttgcgcagg tcgcgcaccg cgggcacgtc ctgcggcaag
    19081 aggtacgaga agtccgcgaa cagcggcagc cggaacgcga tcaggtagag cgcgatcccg
    19141 aggaccgcga cgtagacgcc gatcatcacg aacacccgtc gcttcagcca cgccacgtac
    19201 cggcgcgcga gctgggcggg catctcgttg gacacgccgg cagcctagct cgtttccgcc
    19261 atagatctag acacgtggac gtgaacgtag gcgtgaactt tcgttacgcc gggcctaccg
    19321 cagtgcttgc tccacgtcgc cgtcgccgtc aacgttcacg atgacgatga cgacgatgac
    19381 gcgttacgcg gcgcctacca cagtgcttgc gaacgcttcg gtgcgttatc gcgttccgga
    19441 cgagcttcgg cgcggcggcg gttcttcttg tcctgcatat cgcgcgacat cttttgcagc
    19501 tcgggcgcca gcgcgaacga tgcgatgtgg ccgagctcgg gcgactcgcg cgagagctct
    19561 gcaacggcga gcgcggtgtt cggatcgaca tcgcggccat cgaggcgaac ttcgaagtgc
    19621 aagtgcgggc ctgtcgcgcg ccccgtcgag ccgacctgac cgatgcgcgt gccggcggtg
    19681 agcacgtcgc ccttcttcgt ctcgatgcga cggaggtggc catagcgcgt cacgacgcca
    19741 ttgccgtggt cgacatagat gacgttgccg tagccgcctt ggcgaccggc gaacgcgacg
    19801 acaccatttc ctgccgcgag caccggcgtg ccgggatcgg cgcggtagtc ggcgccgtgg
    19861 tggaagcgct gattcttgcg aatcggatcc tcgcgccagc cgtacccgga gctctccgtg
    19921 cgctcgacag gctccgtcgt caggaccgag agatcgggcg gcgcaggcgc cagcgtcgcg
    19981 agacgcccga cgagcgtgcc gtcgagaaat ggcccagcag acgcagcgct cgaagcgagc
    20041 agcacgaccg tcagcgacaa caacaccagg cgcatgtatc ggaccgaacg cgatcattgg
    20101 tgcgcctaca tgtcgacggt caatgtaggc atgcggcttt tggccgcttg tgagcacgtg
    20161 ccagatcggt tgcagcacaa acaaccgaaa tgtatacggc cggttatgga aacgaaaacg
    20221 cggtggataa cggtaggcat cgcactgctt gccgcattcg cgtttgcgct atccgtgcag
    20281 agtgcgtggt ggacgatgtc gagcgtgatc gtcgggccat tcggcacccg caattgtccc
    20341 ggcggcgcgg atgaatgcgg cttcgcgtgg ctcggtacga atcacgatct gttcatgcgc
    20401 tctgcggttg cgacgcgcgc aggcggctac atcgcgatgt tcgtctacat catggtcgcg
    20461 ggcgcgatcg cggcgaagcg cgttccgtcg ctgctctcga aagcaggcat cgttgcgatc
    20521 ctcaccgcga gcgccgcggc gatcacgttc ttcgtgaagt ttcccggctc gttcggtgag
    20581 ccgtcactcg gcgcgggccc gatcctcttc gtggtcggcg tggtcgccgg cgttatcgca
    20641 tctgtgcgcg tgctgcgtgc gcagcgttga gcgcgacgtc tgcatcatcg aggcgtagtg
    20701 cggtcttcgc ctggtcgcgc aggatgaaga agccgaggac cgtgatgagg accagcgccg
    20761 gcatcatcag catcgcaagc gtgtcgtagc cttcgaacga cagcggcgct tggaggaacg
    20821 cgcacagatc gtagaggccg tgcattgcga ccgcgagcaa gtagccgccg agcaggccga
    20881 gtcccttctt gtcgaagcgc ctgcgcgcgg ccatcgcgcc gatgatgcct gtccagatcg
    20941 cgtggccggg aaccgcgagc acggcgcgga ggatccacgt cgtgagctga ccactgagcg
    21001 actgctgcat cagcaagtag ccgatgttct cgacgagagc gaagccgagg ccggcgcggc
    21061 tgaagtagac gatgccgtcc atgcgctcgt cgaactcggg cttgtgccag atgaacatga
    21121 agatgagagc ggccttgcag ccctcttcga cggcggcggc gatcacgaac gcgtgaaacg
    21181 ccgagccctg gtacgtcatc tgcggctcga tgttcgcgcc gagcttcttc gatagaagca
    21241 tctcgagaat gatcgccggg atgatcgaga gcattccgag gaatgcggcg aggcggcgcg
    21301 tgccgggagg ctctggacgc ttccgatcga ggcggtcgac gagcgccatc gcaatgatcg
    21361 ccgggatggc gccggagagc gcgatctgcg ccgtgtactc gttaccaccc gctgccagta
    21421 tccacgcggc attcatggca gttggcagta tcgactgaat tggcggcggc gcgccaccgt
    21481 ctgagacaca ctaccctcat gctgcgcgtc ctcgccgtct tggttcttct ggtcagcgtc
    21541 gcgcccgttg cacacgcggg tcgcggcacg aacctggtga agtacctgcc cgacgacacg
    21601 aacgtcatcg tgatcgccga cgtgtctcgc gcgcgacgct caccgatttt caagaaagca
    21661 ttcgaggcgg cgcgctccaa gagcgacacg ctcgacaacc tcgcgtcgac actcgcggtc
    21721 gacaagctcg tcgatacgat cgccgtgggc ggcaatggcg ccgcgcagag agcggtcgtc
    21781 gtactcgaag gtcgcgtcga caagctgctc gccgaagcga agaagcaggc gacgaaagag
    21841 gacaagcacg gcggcgtcgc gttctggacg gtgcccgatg gcgaggtcgc agtcgtcgac
    21901 aagaagctcg tgatcgcgag cgccggcgaa atggagcacg tcatcgatcg cgcgcaggac
    21961 aagaaggcca agggccccgc cgcggtgcga acgatcttgg cgagcgcgac ggccaattcg
    22021 agcgtgttcg gcggcgccgt cctcgatgcc gatcagaaga aagaaatggc gaaggagatc
    22081 ggcgccgagc cgcaatgggc ggcgttctcg ttcgcgatgg cccagaagct gacgatcgac
    22141 gccaaggtca agctggccga cgacgcctcc gccgagaagg tggccaagtc catcaacgag
    22201 aagctcggcg cgcccggcga ggacgggacg aaccggagcc gcatcgaggg cttcgtcggt
    22261 aaggacttcg ctgactccat cgtcgtcgac caggaccaca gcttcgcgcg catctcggcg
    22321 acgatgtcgg cagacgaatt cgacaaggtc ttggctctcg tgaagatgtt tatgtaggtg
    22381 ttcccgacgg ccgacccgag ggggtaacag cgggggtggt cggcttgaca cacctagcta
    22441 accagccgat ccttctaggg tgccggagcc atccgcgccg gggatgcccc agcgagtcgg
    22501 ccgctacgag gtcattacgc acctcgcgac cggcggcatg gcgcagattt acctcgcgcg
    22561 gcagagcggg cttggtgcgt tcgaacgtca cgtcgtcctg aagacgattt tgcgcgagcg
    22621 cgcgaccgac cagcgcttcg tcacgatgtt cctcgatgaa gcgaagctcg cggcgacgct
    22681 gaaccaccag aacatcgcgc aggtctacga agtcgatcag gccgacggcg cctacttcat
    22741 ggcgatggag tacgtccacg gcgagaacgc gcgcgcgatt ctcgagacga cgctccgccg
    22801 tggttggacg attcctctcg agctcgccgt gatgatcgtc agtggcgcag ccgccggcct
    22861 tcaccacgcg cacgagcgtc gcggcaagaa cggccagccg ctcaacatcg tgcatcgcga
    22921 tgtgtcgccc gcgaacatca tggtcggcta cgacggcagc gtgaaggtgc tcgacttcgg
    22981 catcgcgaaa gccgaggagc gcgcgaccaa gaccgtcggc ggaacgatca aaggcaagta
    23041 cggctatatg tcgcccgagc agtgcaaggg caagccgatc gatcgacgca gcgacatctt
    23101 cgcgctcggc atctgtctct acgagctcac gacgctgcgc cgcgccttca agggcaacga
    23161 cgatttcgag acgatgaagc gcatcgtcgc gggtgacgtg ctgctgccga gcgtcgcggt
    23221 gcccggttat ccgcgcgagc tcgaagcgat cgtgctgacc gcgatggcaa acgatccgaa
    23281 cgcgcgcttt caatcgggcc aagagatgat cgaggcgctc gacgcgttcg cggtacgcgc
    23341 gaagctaacg ggctcgaaca cggcgatggg ccgcttcatg gtccagctgt tcgggagcaa
    23401 gaaagagcct tggatcgagg caccgagtgg atccgatcgc acgcaggtca ccgagccaag
    23461 cggcgagatg gatccggaca acgacgagaa gacgacgctt atcgagagca agcagcagcc
    23521 cgagtacgga cgcgccgcaa ccgcgacggc gccgggcacg ggcatgggac cgcaggtcgg
    23581 tcgccaatcc ggcgaggtgc agtcactttc ggcttccgcg gcggccgccg attggaatca
    23641 cgacagccgc gccggacgac acagctcgcc gcgtcctggt ttcgatcagc cgcgcacgcc
    23701 accgaatggc tatcagttac cgaacgcggc gatgacgagc acgctcgcgg gaacgggccc
    23761 ggtgccggcg attcctgggc gaattccatc gcgtgccgaa accgagccgg gctcgaagcc
    23821 gctcgtcgac gaccgtcttg cgtggcagca accgcaaaac ccggcgatgc gcgatcatcg
    23881 tctgccgccg cccggcgcga cacggctgcc gacgcagtat caggacccga acttcaagat
    23941 cacgtcgaat cgcggctgga tcatcttggc gattctgatg ctcgcgggcc tcggcgcggg
    24001 cgtgttgatc gcaatgcaaa gcagctgagg atcgtgtcat ggtcgccgca tggcgaccgc
    24061 gacgacccag aacagcgagc gactgcagtc ctacaacccg gcgacgggcg aatcgatcgg
    24121 cagcgtgccg atcatgacgc cggcggaagt cgatgcggct gtcgcgcgtg cccgcgtcgc
    24181 tgcgacgacg tgggggacgc gtagcttcaa agcgcgcgcc gaagaactga ccgcattccg
    24241 catcgcgctc gccgaagccg cggacgagat cgccgagctg ctccatcgtg agaacggcaa
    24301 gccgctgctc gagggctacg tcgaagtgat gatggcgctc tcgcacgtca agcacgcctc
    24361 gatcgtcgcg gaagaagcga tgcgcccgcg caaggtcgcc tcgggtgtcc tcgcgaactt
    24421 ccgcgcgacg atcagctact tcccgctcgg cgtgatcggc gtcatcggcc cgtggaacta
    24481 cccgctgttc acgccgatgg gctcgatcgc gtacgcgctc gcggccggca acgccgtcat
    24541 ctggaagccg agcgagctga cgccgctcat cgcgctcgag atcgagaaga tcgcgaagaa
    24601 gacgttcgcg ctgcccgacc tgctgcaagt cgttaccggt gccggcacca ccggcgcggc
    24661 gctcgcgaag tcggccgtcg acaagatcgc gttcacgggc tcggccgcga ccggcaagcg
    24721 cgtgatggcc gcagcggcgg agcgcctcac gccggtgctc atggagctcg gcggcaaaga
    24781 tccgatgatc gtcgacaccg acgcggatct cgagaaggcc gcggaggccg ctgtgttcgg
    24841 tggtctcacg aactccggcc aagcatgcgt ctcggtcgag cgcgtgtatg tctcggagcc
    24901 cgtctacgat cggttcgtcg acgaagtcgt caagcaagtg cgcgagctca aggtcggcgg
    24961 cgatgacgga cacctcggtg cgatgacgtc accggcgcag gtcgcgatcg tcaaagacca
    25021 tctagaggac gccgtgaaga agggcgccaa ggtcctgacc ggcggtcccg acgcgatcag
    25081 cggcagctat atccagccga ccgtgctcac gaacgtcgat cacaagatga aggtgatgag
    25141 cgaggagacg tttggaccgg tcatcccgat ccagaaggtg aagtcggtcg ac
//
LOCUS       DATLBZ010000058         8563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3004058, whole genome shotgun sequence.
ACCESSION   DATLBZ010000058 DATLBZ010000000
VERSION     DATLBZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3004058"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..583
                     /locus_tag="VMZ53_02945"
     CDS             251..583
                     /locus_tag="VMZ53_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27430.1"
                     /translation="MRLHLVMDKTTGETRVRGSDGAVHTTAEIDAARRRAERGDEAAR
                     AFLKTVELPKGTTAAQLIHDCPECRAAMERGEKPTFLDPPQVERANRHGLFRRRPRWR
                     DLKRTAGR"
     gene            966..1505
                     /locus_tag="VMZ53_02950"
     CDS             966..1505
                     /locus_tag="VMZ53_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HUS27431.1"
                     /translation="MARLVFGMMQSLDGYVDDAAGTLCMPVPGPALFRQFIDVVAGES
                     GAIYGRRIYELMRYWEVDRPEWDAAQREFAIAWRRLPKWVASRTLESVGPNATLIRGD
                     VVEFVRDLKSKMDGELEVAGPELAGVVAPLIDEYRLYLRPFVLGGGKAFFVGGRPALR
                     LVAMDRIDEDAVKLTYVPR"
     gene            complement(1840..2856)
                     /locus_tag="VMZ53_02955"
     CDS             complement(1840..2856)
                     /locus_tag="VMZ53_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27432.1"
                     /translation="MKRLLLILMLLFATVADAAPRLEHLPRKGRIDMAYEPGLESTAR
                     ELQASADATLRRIADDLVDLPAPAVVHIQLVNDASSLAAVAPEGRGAPAYAIGVAYPD
                     LGVLSVAVRKGSNVTDPAQTLRHELAHLALGAAFAGKPVPHWLHEGFAYQHSAEWSWD
                     TTETLAGMTWMNNVIPLEQLDASFPDQELPAHQAYAESYDFVGYLSRRGRYEDTDDDG
                     NRWPFRKFLSLIGNGNTVDEAAVKAYGRPLKDLFDEWQSDLSKRYFMGPIGLLGLAVW
                     ILCALLLTLAYIRKRRQRRRRFGEWEREDREREAEIARIEALLARHEEDAFDDPDGPP
                     KLVN"
     gene            complement(2853..4046)
                     /locus_tag="VMZ53_02960"
     CDS             complement(2853..4046)
                     /locus_tag="VMZ53_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27433.1"
                     /translation="MGPQQVLRAAACLAVTASIASAAPRTDRVAVIDLGPDTADASVR
                     GKLASAVVDAGLEPVYGDGIEDALAGIDVERDAVSLAAALADAREKFGALDCPGAVTD
                     AQTAISIGAGRQAAGLAVPELPRAWAYVLLCADRTSDTPLALVAASAIRTLGGSKDVD
                     AKILAKYPDVDALSNREVIEIEIKADAPGADIWIDFKRAGISPLKLPLAAGPHVIAAA
                     KGSQRGVLTGTVVRKQPVLTVPLADQAGKWTSIAKRVASWKGALPSPAELATLMTSVN
                     ARVAIVRKGSTIEAWGHAGLAEPLRRLGGADDGVRTLDDAAGLVALISDRIQTWSDRA
                     PDPDQPLLTESSLAARARVIKEEEKPAAWWVYATIGGAILAGALVIYAHDSADNTQHV
                     EIHYP"
     gene            complement(4001..4258)
                     /locus_tag="VMZ53_02965"
     CDS             complement(4001..4258)
                     /locus_tag="VMZ53_02965"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS27434.1"
                     /translation="MGGDPSPITISNISLGGALIAAGTRYPMGQRVTISFVIPTLEEA
                     IEVGATIRWSDDKATGLQFDGLRAREVWALNKYFEQLPALP"
     gene            complement(4433..5428)
                     /locus_tag="VMZ53_02970"
     CDS             complement(4433..5428)
                     /locus_tag="VMZ53_02970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUS27435.1"
                     /translation="MRTLALLLIALTAAPAFAQPAPPRDHRTSPNAGKKVQLERVVAV
                     VNDSIILSSELDARLVPVMAEAQQIQDPAERRRRIAKLASQVLDEMVNEELIVQAADA
                     AHVEVDSSEVQAALDEIKQQNGLDDAGLAQALSAQGYTMQNYRMDLRRQLLRLRAQNQ
                     LVAPKVQITEEDVRARYDQMQRRSDAVSAIYLSHILIKLPDHPTEQQIADAKAKAAKA
                     IERVKGGEPFLDVAKQVSDDQNVELGWFQRGSLTPETEQVVFSMEKGDVRGPYSEAGG
                     VRVFFVSDVKNSSLKPYAEMKDQLKNELRRRELDKQTAAWVEELRKKAYIDIKLQ"
     gene            complement(5447..6547)
                     /locus_tag="VMZ53_02975"
     CDS             complement(5447..6547)
                     /locus_tag="VMZ53_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HUS27436.1"
                     /translation="MQKVGVFLLVVLAVAGACRKTPGDRSKLDRVHPAGAADPPQSSE
                     ELQTPLAKIDDVTITLGEFQERINRQSPYIRARYTSLEQKKEFLDSLVRFEVLAKEAY
                     RRGLDKDPEVVRTMKQVMIQKLMRDEFDAKVTADTVPDADMQAYYNANLDEYVKPEEV
                     RVSAIILKNKAQAERVLVEAKGEAGKTNKGFRDLVMKYSQDEDTKLRGGDLRYLELTN
                     KEVPAPVVKGAFALANTGDVSPVVDGGNGQFYILKQTGRRKQMTKSFDDAKPQIRNKL
                     FREKRLKAQQDFIDGLRAKAKVEINEANLAKVRVDTSQNAIPDDGHGHDLVPSTGQGS
                     DLQPGPPPADLSPPAGVNPSGSTPAPPGQPNP"
     gene            complement(6550..>8563)
                     /locus_tag="VMZ53_02980"
     CDS             complement(6550..>8563)
                     /locus_tag="VMZ53_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS27437.1"
                     /translation="TVSILQTTAVGAPTPKATEIDALHHEYDGGTLYLPVYRLGEVQR
                     YVGAEGHAPKIDKLGGVTWEATQSKVKRHVRVLAEELLQIYAQRKSLPGHTFPPPDDS
                     YRELEATFPFDETPDQLDAINAVMRDMEAPRAMDRLVCGDVGYGKTEVALRAVFKAVQ
                     GGKQACMLAPTTVLVEQHFRSLNERFKGFGLNIGKLSRFQSKGEQVDTVKKITEGKVD
                     VVVGTHRLLSADVRFKDLGLLVIDEEQRFGVAHKERIKKTKTQVDVLTLTATPIPRTL
                     HLAMAGLRDLSIIATPPADRRAVRTFVSRVDDATIREAIERELSRGGQIFWITPTIGQ
                     MGQQEADHRLPNESRPQRRTKPRDDDRTIEEWADYIRSLVPKARVGIGHGSLSSEQLE
                     KVMVQFVQGELDVLVATTIVESGLDIPRANTMFVSRADAFGLAQLYQLRGRIGRSKER
                     AYCYLLVPEPEHITEEARRRLEALQRFTELGAGFQIASQDLEIRGGGELLGAKQSGSI
                     AAVGFDQYVKMLEAAVSELKGEPIHSEVDPELLIEIPGFIPDDYVPDPGQRLELYKRL
                     SAVVDDEELKSVMNEISDRYGPLPGDVVLLGELMGIKAIARKLGVLALEISSSRVAIA
                     FAPDDARLANAVSASGWRRLPDGRYATQPPARKALLDVQARATRSA"
BASE COUNT         1328 a         2681 c         2972 g         1582 t
ORIGIN      
        1 ggcgcgcgcc cctggccgtt tgaaaggccg gggcgcggcg ctccccgccg gcccggcgct
       61 ctcgccactc atgtgaggag ctgacgagac gcgcgacgta ctcaggggtt ggcgttcaaa
      121 cgcgaaacgg cttccgcgtg agcgccggct actcgcgtgg cctggcgtcc agcattcgga
      181 cactccgcga gcgccgccga ggaagaacgc gcgcttagag gcggcacagc ggctgctcta
      241 cgggctgtgc atgcgactac acctcgtgat ggacaagacc accggagaga cgcgcgttcg
      301 cggaagcgat ggcgccgtgc ataccaccgc cgagatcgat gcggcacgcc gacgagccga
      361 acgcggcgat gaagcggcgc gcgcgttttt gaagacggtc gagctgccga aagggacgac
      421 cgctgcgcag ctgatccacg actgcccgga gtgccgcgca gcgatggagc gcggcgagaa
      481 gccaacgttc ctcgatccgc cgcaggtcga gcgcgcgaat cgccacggct tgttccgccg
      541 ccggcctcga tggcgcgatc tgaagcgcac tgcgggtcgc tagatcgcgc ctcggcgatc
      601 gcgagctcga gctcgccatg cgccgctagc tcgcgcatcg gcgagcgcaa gttgaagcgc
      661 gccgtgcacg gctagatcgc gcatcggcgt ctcctcgaaa gagatgtcga agagcgagcg
      721 tttcctcgga agagacgctc gaccgcaagc ggcgatcggc aagcgcgagt tgaatcgcga
      781 cgtgcgccgc gaatcggcgt ctcctcgaaa gacatgtcaa cgagtgtttc ctcgaaagag
      841 acgctcgacc acaagctcgc gcatcggcac atcctcgaaa gacgtgccga cgagcgtttc
      901 ctcgaaagag acgctcgacc gcagctgcga tcggcgcatg cccctgcgcg aggtagagtg
      961 cgctcatggc caggctcgtc ttcggcatga tgcaatcact cgacggctac gtcgacgacg
     1021 cagcggggac gctttgcatg ccggtgccgg ggccggcgtt gtttcggcag ttcatcgacg
     1081 tcgtcgccgg ggagtcgggg gcgatttatg gccgccgcat ctatgaattg atgcggtatt
     1141 gggaggtcga ccgaccggag tgggacgcgg cgcagcgcga gttcgcgatt gcgtggcgac
     1201 ggctcccgaa gtgggttgct tcgcgcacgc tcgaatcggt cggaccgaac gcgacgctga
     1261 tccgcgggga tgtcgtcgag tttgtgcgcg acctgaagtc gaagatggac ggcgagctcg
     1321 aggtcgcggg gccggagctt gcgggtgtcg tcgcgccgct tatcgacgag taccgcctct
     1381 atttgaggcc gtttgtgctc ggcggcggta aggcgttctt cgtcggcggg aggccggcac
     1441 tgcgcctcgt tgcgatggat cgcatcgacg aggatgccgt gaagctgacg tacgtgccgc
     1501 ggtgacaact cgcgttgcct ggcgtccagc attcggacgt cgtggggcgc ggcgtgcgag
     1561 gggacgcggg gttagcgacg gcactgtcgt tgctgctgtc gcgacgcatg cgtcaccatc
     1621 atcttgcagc agcacccgaa ttccaaatca ctcccattgc tagcgtcgag gaggggcgtc
     1681 agcacatcga cgattgccct gactgccgcg ccgcgcgaga gcgtggcgag gagccgctga
     1741 ttgtttccgg agaggagctc atgcgcctga cgcggagcgt gcttcgacgc cggcggccgc
     1801 gttggcggga cttgaagcgc gcgctgcgcc gatagcggct cagttcacga gcttcggcgg
     1861 gccgtcggga tcgtcgaacg cgtcttcttc gtggcgcgcg agcagcgcct cgatgcgtgc
     1921 gatttcggcc tcgcgctcgc ggtcttcgcg ttcccactcg ccgaagcggc ggcggcgttg
     1981 gcggcgcttg cggatgtacg cgagcgtgag cagcagcgcg cagaggatcc agaccgcgag
     2041 gccgagcagg ccgatcggcc ccatgaagta gcgcttcgag aggtcggact gccactcgtc
     2101 gaagagatcc ttgagtgggc ggccgtacgc tttgactgcc gcttcgtcga cggtgttgcc
     2161 gttgccgatc agtgagagga atttgcggaa cggccagcgg ttgccgtcgt cgtcggtgtc
     2221 ttcgtaacga ccgcgccgcg agaggtagcc gacgaagtcg tagctctctg cgtacgcttg
     2281 gtgggcgggg agctcttgat cggggaagga tgcgtcgagc tgctcgagtg ggatgacgtt
     2341 gttcatccac gtcatgccgg cgagggtttc ggtcgtgtcc cagctccatt cggcggagtg
     2401 ttggtacgcg aagccttcgt gcagccaatg agggactggc ttgcccgcga atgcggcacc
     2461 gagcgcgagg tgcgcgagct cgtggcggag cgtttgcgcg gggtcggtga cgttggagcc
     2521 tttgcggact gcgacggaga gcacgccgag atcgggatag gcgacaccga tcgcgtatgc
     2581 cggtgctccg cgaccttccg gcgcgactgc cgcgagcgac gacgcatcgt tgacgagctg
     2641 gatgtggacg accgccggcg ccggcagatc gacgaggtcg tccgcgatcc ggcgcagcgt
     2701 cgcgtcggca ctggcttgga gctcgcgcgc ggtcgactcg aggcctggct cgtacgccat
     2761 gtcgatgcgg cctttgcgcg gcagatgctc gaggcgcggg gctgcgtccg cgaccgtcgc
     2821 gaagagcagc atgagaatga gcagcaggcg cttcacgggt agtgaatctc cacgtgctgc
     2881 gtgttgtcgg cgctgtcgtg cgcgtagatg acgagcgcgc cggcgaggat cgcgccaccg
     2941 atggtcgcgt agacccacca cgccgcgggc ttctcttctt ccttgatgac gcgcgcgcgt
     3001 gcagcgagcg agctctcggt gagcagcggc tgatcgggat ccggcgcgcg atcggaccac
     3061 gtctggatgc gatcggagat gagggcgacg aggccggcgg cgtcgtcgag agtgcggact
     3121 ccgtcatcgg cgccgccgag gcgacgcagc ggctccgcga gacccgcgtg gccccacgct
     3181 tcgatcgtcg agcctttgcg gacgatggcg acgcgcgcgt tgacggaggt catcagagtt
     3241 gcgagctcgg ccggcgaggg gagggcgccc ttccagctcg cgacgcgctt ggcgatcgac
     3301 gtccacttgc ccgcttggtc ggcgagcggc accgtgagga cgggctgctt gcgcacgacc
     3361 gtgccggtga gcactccgcg ctgcgagcct tttgcggcgg cgatgacgtg cgggcctgct
     3421 gcgagcggaa gcttcagcgg cgagatgccg gcgcgcttga aatcgatcca gatgtcggca
     3481 cccggggcgt cggccttgat ctcgatctcg atcacctcgc gattcgagag ggcgtcgacg
     3541 tcgggatact tcgcgaggat cttcgcatcg acgtccttcg agccgccgag cgtgcgaatt
     3601 gccgaggccg cgacgagtgc gagcggcgtg tcactcgtgc ggtcggcgca gagcagcacg
     3661 tacgcccatg cgcgcggcag ctccggcacg gcgaggcccg cggcctgacg accggcaccg
     3721 atcgagatcg cggtctgcgc gtccgtgact gcgcccgggc aatcgagcgc gccgaacttc
     3781 tcccgtgcat cggcgagcgc ggcggcgagc gagaccgcat cgcgttcgac atcgatgcca
     3841 gcgagcgcat cctcgatccc atcgccgtag acaggctcga gccccgcatc gacgacggcg
     3901 gatgcgagct tgccgcggac cgacgcgtcg gcggtgtccg ggcctagatc gataacagcg
     3961 acgcgatcag tgcgcggcgc ggcgctggcg atgcttgccg ttacggcaag gcaggcagct
     4021 gctcgaagta cttgttgagg gcccatactt cacgcgcccg gaggccgtcg aattgtaagc
     4081 ccgttgcttt gtcgtcggac cagcgaatgg tggcgccgac ctcgatcgct tcttcgagcg
     4141 tcggaatcac gaacgagatc gtgacccgct gccccatcgg gtaccgcgtg cccgctgcga
     4201 tcagcgcgcc accgagcgag atgttgctga tcgtgatcgg actggggtcg ccgcccattg
     4261 ccagtttccc ggccagagat acagcgtgac gagtggatgt acggcgattg tcggtcaagg
     4321 cagtttctcc cgagtccagc cattctatcg gccggctgaa gaaacaccat cgggtccacg
     4381 ctgaaagcat ggatccgaaa agccgtgatg cacctacctg aagaggtggc gcctactgca
     4441 gcttgatgtc gatgtaggcc ttcttacgga gctcttcgac ccatgcggcg gtctgcttat
     4501 cgagctcacg ccggcgcagc tcgttcttca gctgatcctt catttcggcg tacggcttca
     4561 gcgaggagtt cttgacatcg ctcacgaaga acacgcggac gccgcccgct tcgctgtacg
     4621 ggccacgcac gtcgcccttc tccatcgaga acacgacctg ctcggtttca ggcgtcaggc
     4681 tgccgcgctg gaaccagccg agctcgacgt tctgatcgtc ggagacctgc ttggcaacat
     4741 cgaggaacgg ctcgccgcct ttgacgcgct cgatcgcttt ggcggccttc gccttggcgt
     4801 cggcgatttg ctgctccgtc ggatgatcgg gcagcttgat gaggatgtgc gagaggtaga
     4861 tggcgctgac ggcgtccgag cggcgctgca tctgatcgta gcgagcgcgg acgtcttctt
     4921 ccgtaatctg cacttttggc gcgacgagct ggttctgcgc gcggaggcgg agcagctgac
     4981 gccggagatc catgcggtag ttctgcatcg tgtagccctg cgccgagagc gcttgcgcga
     5041 ggccggcatc gtcgaggccg ttctgctgct tgatctcgtc gagcgcggcc tgcacttcgg
     5101 aggagtcgac ctcgacgtgc gcggcgtcgg ccgcttgaac gatcagctct tcgttgacca
     5161 tctcgtcgag gacctggctc gcgagcttcg cgatgcggcg acgccgctcg gctggatctt
     5221 gaatctgctg cgcttcggcc atcaccggca cgaggcgggc atcgagctcg ctcgagagga
     5281 tgatcgaatc attgacgacc gcgacgacgc gctcgagctg caccttcttg cccgcgtttg
     5341 gcgacgtgcg gtgatcgcga gggggagccg gttgcgcgaa cgccggagcg gcggtcaggg
     5401 cgatgagcag aagtgcaagc gtgcgcacgg agataggacc ttctcgctag ggattgggtt
     5461 gcccgggagg ggccggagtg gagccggacg gatttactcc ggccggcggg gaaaggtcgg
     5521 cggggggtgg acccggttgt agatcactac cttggccggt gctggggacg aggtcgtgcc
     5581 catggccgtc gtcgggaatg gcgttctgcg atgtgtcgac gcgcactttt gccaggttcg
     5641 cctcgttgat ctcgaccttc gccttcgcgc gcagaccgtc gatgaaatcc tgctgcgcct
     5701 tgagccgctt ctcgcggaag agcttgttgc ggatctgcgg cttcgcatcg tcgaagctct
     5761 tcgtcatctg cttgcggcgg cccgtctgct tgaggatgta gaactggccg ttgccgccgt
     5821 cgacgaccgg cgagacatcg cccgtgttcg cgagcgcgaa cgcgcccttc acgacggggg
     5881 cgggcacttc cttgttcgtc agctcgaggt agcggaggtc gccgccgcgc agcttcgtgt
     5941 cctcgtcttg cgagtacttc atgaccaggt cgcgaaagcc cttgttcgtc ttgcccgctt
     6001 cacctttggc ttcgacgagg acgcgctctg cttgcgcctt gttcttgagg atgatcgccg
     6061 agacgcggac ttcctcgggt ttgacgtact cgtcgaggtt cgcgttgtag tacgcctgca
     6121 tgtcggcgtc ggggacggtg tccgcggtga cctttgcgtc gaactcgtcg cgcatcagct
     6181 tctggatcat cacctgcttc atcgtgcgga cgacttcggg atccttgtcg agaccgcggc
     6241 gatacgcttc tttcgcgagg acctcgaagc gaacgagcga gtcgaggaac tccttcttct
     6301 gctcgagcga cgtgtagcgc gcgcggatgt acggcgactg ccggttgatg cgctcttgga
     6361 actcgccgag cgtgatcgtg acgtcgtcga tcttggcgag cggagtctgc agctcttcgc
     6421 tgctctgcgg cgggtcggcc gcgcccgcgg gatggacgcg atcgagcttg ctgcgatcgc
     6481 ccggcgtctt tcgacacgca cccgccaccg cgagcacgac gagtagaaag accccaacct
     6541 tttgcatggt tacgctgatc gagtagcacg ggcctggacg tccaacaagg ctttcctggc
     6601 cggtggctgc gtcgcataac gcccgtccgg caacctgcgc caaccggagg cgctaacggc
     6661 atttgcgagg cgcgcatcgt cgggggcaaa cgcgatcgcg acgcggctcg acgagatttc
     6721 gagtgccagc acgccgagct ttcgcgcgat ggccttgatg cccatcagct cgccgagcag
     6781 gacgacgtcg ccgggcagcg ggccatagcg atcgctgatc tcgttcatca ccgacttcag
     6841 ctcctcgtcg tcgacgacgg ccgacagccg cttgtagagc tcgagccgct ggcccggatc
     6901 cgggacgtaa tcgtcgggga tgaagccggg gatctcgatg agcagctcgg ggtcgacctc
     6961 gctgtggatc ggctcgccct tgagctcgga caccgcggcc tcgagcatct tcacgtattg
     7021 atcgaagccg accgcggcga tcgagcccga ttgctttgcg ccgagtagct cgccgccgcc
     7081 gcggatctcg agatcctgcg acgcgatctg aaagcccgcg ccgagctccg tgaatcgctg
     7141 cagcgcttcg agccggcgcc gcgcttcctc ggtgatgtgc tcgggctccg gcacgagcag
     7201 gtagcaatac gcgcgctcct tgctgcgacc gatgcgaccg cgaagctgat agagctgcgc
     7261 gaggccgaac gcgtccgcgc gcgagacgaa catcgtgttg gcgcgcggga tgtcgaggcc
     7321 gctttcgacg atcgtcgtcg cgacgagcac gtcgagctcg ccttggacga actgcaccat
     7381 caccttctcg agctgctccg agcttagcga gccgtggccg atcccgacgc gcgcctttgg
     7441 cacgagcgag cggatgtagt cggcccactc ttcgatcgtc cgatcgtcat cgcgcggctt
     7501 ggttcgacgc tgcggccgcg actcgttggg caagcgatgg tcggcttcct gctggcccat
     7561 ctggccgatc gtcggcgtga tccagaagat ctggccaccg cgcgaaagct cgcgctcgat
     7621 cgcttcgcgg atcgttgcat cgtcgacgcg cgacacgaac gtgcgcacgg cccgtcgatc
     7681 cgcgggtggc gtcgcgatga tcgatagatc gcgcagtcct gccatcgcga ggtgcagcgt
     7741 gcgcggaatc ggcgtcgcgg tgagcgtcag cacgtcgacc tgcgtcttgg tcttcttgat
     7801 ccgctccttg tgcgcgacgc cgaatcgctg ctcctcgtcg atcacgagca agccgagatc
     7861 tttgaagcgg acatcggcgg agagcaggcg gtgcgtaccg acgacgacgt cgactttgcc
     7921 ctcggtgatc ttcttcaccg tgtcgacttg ctcgcccttg ctctggaagc gagagagctt
     7981 gccgatgttg aggccaaaac ccttgaagcg ctcgttgagc gagcggaagt gctgctcgac
     8041 gagcaccgtc gtcggcgcga gcatgcacgc ttgcttgccg ccttgcaccg ccttgaacac
     8101 ggcgcgcagc gcgacttctg tcttgccgta gccgacgtcg ccgcagacga ggcgatccat
     8161 cgcacgcggc gcttccatgt cgcgcatcac cgcgttgatc gcgtcgagct gatcgggcgt
     8221 ctcgtcgaac gggaacgtcg cttcgagctc gcgatatgaa tcgtcgggcg gcgggaacgt
     8281 gtgacccggc agcgactttc gctgcgcgta gatctgcagc agctcctcgg cgagcacgcg
     8341 gacgtgccgc ttgaccttgc tctgcgtcgc ttcccaggtg acgccgccga gcttgtcgat
     8401 cttcggcgcg tgaccttccg cgccgacgta gcgctgaact tcgccgaggc gatagacggg
     8461 cagatacagc gtgccgccat cgtactcgtg gtggagcgcg tcgatctcgg tcgcctttgg
     8521 tgtcggcgcg ccgacggctg tcgtctgcaa gatcgagacg gtg
//
LOCUS       DATLBZ010000059         4401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4515977, whole genome shotgun sequence.
ACCESSION   DATLBZ010000059 DATLBZ010000000
VERSION     DATLBZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4515977"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VMZ53_02985"
     CDS             <1..1366
                     /locus_tag="VMZ53_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HUS27438.1"
                     /translation="FCTQGCPLGNPIPDFAHAIWTDRWHDAHKQLAVTNEFPEFTGRL
                     CPAPCEGACVLAINSDPVTIEHLERSIIERAFAEGWVQPRPPLTRTNKRVAIVGSGPA
                     GLAAAAHLNRAGHTVTVYDAAPRPGGLLRYGIPDFKMEKHVIDRRLAILEAEGVQFRC
                     NVTIGADHTAVRYATSPRTGLHVTPPNGVPAPTSVTWKQLRAEHDAVIIAIGAQRARD
                     LDVPGRNLTGVVFAMDFLVEQNEVVATARQASTRDVRGKHVVILGGGDTGSDCLGTAL
                     RQGAASVRQIELMPAPPESRSESNPWPTWPYVFRTSSSQEEGGERTFAFRTTKLEGDA
                     TGQLVALLGMRVEVRDGKLVDVPSTEAPDGFTLGEGGQLRLACDALILALGFTGPDAT
                     PLVEQLGISLDARKAIVVDKQYATNVKGVFCAGDAHRGASLIVWAIAEGRDTARAVDA
                     FLRS"
     gene            1390..2379
                     /locus_tag="VMZ53_02990"
     CDS             1390..2379
                     /locus_tag="VMZ53_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27439.1"
                     /translation="MRSGWLLAAGLAGAIGAAGAAGCYQPTLHEGAPCGEGATCPSGQ
                     ECRAGTCFFTSTPADANLFGDAIELDSSMITVDAPPDGPPYIAWGTPVELTTLELAED
                     AEDDPSVTTNKLTIVLSAKIVAAPNDRDLYIGTRSAMTDSFVVTPLTVLNATPSGETS
                     GEISADGSTLYFVSDRVTAGDGDVYVSTQTGGVWAAPTLVPQLSVGNVNDIAISPDGL
                     TAVIVHGAFYVHTRASTTAAWSAGVAHSELTTGLASATAPTITNGAQTIYFHAGATRD
                     VYTSHRNANGTFPTATPVAELNTTASRDAAPFVSQTDNYMIFERDYDIFETTR"
     gene            complement(2405..2554)
                     /locus_tag="VMZ53_02995"
     CDS             complement(2405..2554)
                     /locus_tag="VMZ53_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27440.1"
                     /translation="MKRTGETTAGASITVGILAAILKLGLTGALVLGLIVGCTMALIV
                     VANRR"
     gene            complement(2695..3999)
                     /locus_tag="VMZ53_03000"
     CDS             complement(2695..3999)
                     /locus_tag="VMZ53_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HUS27441.1"
                     /translation="MSQPPPGSMGAPYLGEALKFLKDPFEFTLARTRQHGNVWKTRIL
                     GDTVVFFAGPKAFSVFMDPENFTRQNGSPKFLQEILHPDAVPFLDGDRHKTRKRLLLS
                     AFKDEALDSYLPGMTRIFKRFVSSWTSERAVIGDLGQLGFDIADMLFAAADPDASNKD
                     AAADFQLMNRGTFAPPVNLPFTAYGKAIKARDRLRAYIKNAVATKDGKGSALGVLKAA
                     RGPNGEQLSNTELEIELLHFYFAAQGGLTAAIAWLLVVLGEHPDLAARLRTEADSVLT
                     TDTPTLEQVKRLGQTRAVSREVLRSYPIVPTTFIGIAKKDLEVDGYAIRAGWKGAGAI
                     WATLQDGSTFKDPTSFDGARLGDESFNALPTGAFVPQGGGPADGHRCAGEILTQIAMA
                     AFLGWFVKHYDLSWPAQDAKPAGAGIGPLPKSGVRLKITPRT"
     gene            complement(4021..>4401)
                     /locus_tag="VMZ53_03005"
     CDS             complement(4021..>4401)
                     /locus_tag="VMZ53_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUS27442.1"
                     /translation="DYSTQDFATECRKLTDKRGVDAVIEHVGGDVFASSIKAVKNGGR
                     IVTCGATAGFHPNIDLRHIFFRQVEVLGSTMGSKADLLTVLGHIASGALKPVVHEVLP
                     LERAADAHRILEQRKAFGRVVLLP"
BASE COUNT          741 a         1472 c         1460 g          728 t
ORIGIN      
        1 gttctgcacg caaggctgcc cgctcggcaa cccgatcccg gacttcgcac acgcgatctg
       61 gaccgatcgc tggcacgacg cccacaagca gctcgctgtc acgaacgagt tccccgaatt
      121 caccggccgc ctctgccccg ctccatgcga aggcgcgtgc gtcctcgcga tcaactccga
      181 tccagtcacg atcgagcacc tcgaacgctc gatcatcgag cgcgcgttcg ccgaaggctg
      241 ggtgcaacct cggcccccac tgacacgcac gaacaagcgc gtggcgatcg tcggctccgg
      301 ccctgccggc ctcgccgcgg ccgctcacct caatcgcgca ggccacacgg tcactgtcta
      361 cgatgcggca ccgcgccccg gcggcctgct ccgctacggc atccccgact tcaagatgga
      421 gaagcacgtc atcgatcgtc gcctcgccat cctcgaagcc gaaggcgtgc aattccgctg
      481 caacgtcacg atcggcgccg atcacacggc cgtccgctac gcgacgtcgc cccgcacggg
      541 cctgcacgtc acaccaccga acggagtccc cgccccgacg agcgtcacct ggaagcagct
      601 ccgcgccgag cacgacgcgg tgatcatcgc gatcggcgcg caacgcgctc gcgacctcga
      661 cgttccaggc cgcaacctca cgggcgtagt cttcgcgatg gacttcctcg tcgagcaaaa
      721 cgaggtcgtc gcgaccgccc gccaagcatc aacgcgcgac gtgcgcggga agcacgttgt
      781 cattcttggt ggcggcgata cagggtcgga ctgcttgggg acggcgctgc ggcagggcgc
      841 ggccagcgtg cggcaaatcg agctgatgcc ggcaccgccc gagtcgcgga gcgagagcaa
      901 tccgtggccc acgtggccct acgtgtttcg gacgtcgagc tcgcaggaag agggcggcga
      961 gcgcaccttt gcatttcgca cgacgaagct cgagggtgat gccaccggcc agctcgtcgc
     1021 gctactgggc atgcgagtcg aggtgcgcga cggcaagctc gtcgatgtgc cgagcaccga
     1081 ggcgccggat ggcttcacgc tcggcgaggg cgggcagctg cgcttggcgt gcgatgcgct
     1141 gattcttgcg ctcgggttca cggggcccga cgcaacgccg ctggtcgagc agctcgggat
     1201 ttcgctcgat gcgcggaagg ccatcgtggt cgacaagcag tacgcgacga acgtaaaggg
     1261 cgtgttttgt gcgggagatg cccaccgagg ggcctctctg atcgtgtggg cgatcgcgga
     1321 aggtcgcgat accgcacgcg ccgtcgacgc ttttctgcgc agctgacgga atctgagtac
     1381 gatagaggga tgaggtctgg ctggctcctc gctgcgggtc tcgcaggcgc catcggcgcc
     1441 gcgggcgccg cgggctgcta ccagccgacg ctgcatgaag gcgcgccgtg tggtgagggc
     1501 gcgacatgcc cgagcggcca ggagtgccgc gcaggcacct gcttctttac gagcacgccg
     1561 gcggacgcga acctgtttgg cgacgcgatc gagctcgact cgtcgatgat cacggtcgac
     1621 gcaccacccg atggcccgcc gtacatcgcg tggggcacgc ccgtcgagct gacgaccctc
     1681 gagctggccg aggatgccga ggatgatccg tcggtcacga cgaacaagct cacgatcgtc
     1741 ctcagcgcga agatcgtcgc ggcgccaaat gaccgcgacc tctacatcgg cacgcgctcg
     1801 gcgatgacgg acagcttcgt ggtcacgccc ctcaccgtgc tcaacgcgac gccgtccggc
     1861 gaaacatcgg gcgagatcag cgcagatggc tcgacgctct acttcgtctc cgaccgcgtg
     1921 acggctggcg acggtgatgt gtacgtatcg acgcagaccg gcggcgtgtg ggccgcgccg
     1981 acgctggtcc cgcaactatc tgttggcaac gtgaatgaca tcgcgatcag ccccgacggg
     2041 ctcaccgcgg tcattgtcca cggcgccttc tacgtccaca cgcgtgcatc gacgacagcc
     2101 gcctggagcg caggcgtcgc ccacagcgag ctcaccacgg gtctggcgag tgcgacggct
     2161 cctacgatca cgaatggcgc acagaccatt tactttcatg cgggcgccac gcgcgatgtc
     2221 tacacatcac atcgcaatgc gaacggaacg ttcccgacgg ccacgcccgt tgcggagctg
     2281 aacacgacgg cctcgcgcga cgcggccccg ttcgtgtcgc agaccgacaa ctacatgatc
     2341 ttcgagcgcg actacgacat ctttgagacg acgcgctgac acccggctac cgcggtaggc
     2401 gcagtcagcg gcggttcgca acgacgatca gcgccatcgt gcatccgacg atcaagccga
     2461 gcacgagcgc gcccgtgagg ccgagcttca agatcgcggc gaggatgccc accgtgatgc
     2521 tcgcgcccgc tgtcgtttca ccggtccttt tcatgcgcat cgacgagtgc acggcacata
     2581 ccgggggatc ggcgctgcaa cgcgcgcaaa tgcctgaccg gaaagtcgat cagccgttgc
     2641 gagcagtgat cagaacgtcg ccccgcgttt actgacgccg cgagctgcca gtcgttacgt
     2701 gcgcggcgtg atcttcaggc gcacgccact cttcgggagc ggaccgatgc cggcgccggc
     2761 gggcttcgca tcttgcgcag gccacgagag gtcgtagtgc ttcacgaacc agccgaggaa
     2821 ggcggccatc gcgatctgcg tgaggatctc gcccgcgcag cggtggccgt cggctgggcc
     2881 tccgccttgc ggcacgaacg cgccggtcgg gagcgcgttg aacgactcat cgccgaggcg
     2941 agcgccgtcg aacgaggttg gatccttgaa ggtcgagccg tcttggagcg tcgcccagat
     3001 cgcgcccgcg cctttccagc cggcgcggat cgcgtagccg tcgacctcga ggtctttctt
     3061 cgcgatgccg atgaacgtgg ttggcacgat cggatacgag cgaagcacct cgcgcgagac
     3121 ggcgcgtgtc tgaccgagcc gcttcacttg ctcgagcgtc ggcgtgtcag tcgtgagcac
     3181 gctgtccgcc tcggtgcgca gccgcgcggc gagatccgga tgctcgccga gcacgacgag
     3241 tagccacgcg attgcggcgg tcagcccgcc ctgcgcggcg aagtagaagt gcagcagctc
     3301 gatctcgagc tcggtgttcg agagttgctc gccgtttggg ccgcgcgctg ctttgagcac
     3361 gccgagcgcc gagcctttgc cgtccttcgt cgcgacggcg ttcttgatgt atgcacgcag
     3421 ccggtcgcgc gctttgatcg ccttgccgta cgcggtgaat ggcaggttga ccggcggcgc
     3481 gaacgtgccg cgattcatca gctggaaatc ggcggccgcg tccttgttgc tcgcgtctgg
     3541 gtcggccgcg gcgaacagca tgtccgcgat gtcgaagccg agctggccga ggtccccgat
     3601 caccgcgcgt tcgctcgtcc agctcgagac gaaccgcttg aagatgcgcg tcatgccggg
     3661 caggtagctg tcgagcgctt cgtccttgaa tgccgagagc agcaggcgct tgcgcgtctt
     3721 gtgccgatcg ccgtcgagaa acgggacggc gtcgggatgc aggatctctt gcaggaactt
     3781 cggcgagccg ttctggcgcg tgaagttctc cgggtccatg aagaccgaga acgctttcgg
     3841 cccggcgaag aacacgacgg tgtcgccgag gattctcgtc ttccagacgt tgccgtgctg
     3901 cctggtgcgg gcgagcgtga attcgaaggg gtccttgagg aacttcaggg cttcgccgag
     3961 gtacggcgcg cccatcgacc cgggaggcgg ctgactcatg gtcggccata gtacgaccaa
     4021 ctacggcaag aggacgactc gaccgaaagc ttttcgttgc tcgaggatgc ggtgcgcatc
     4081 ggccgcgcgc tcgagcggca gcacctcgtg cacgaccggc ttcagcgctc ccgacgcgat
     4141 gtggcctagc accgtgagga ggtcggcctt gctgcccatc gtggagccga ggacttcgac
     4201 ttggcggaag aagatgtggc gcagatcgat attgggatga aagcccgcgg tcgcgccgca
     4261 cgtgacgatg cgcccgccgt tcttcaccgc cttgatgctc gatgcgaaca cgtcgccgcc
     4321 gacgtgctcg atcaccgcat cgacgccgcg cttgtcggtg agcttgcggc actcagtcgc
     4381 gaagtcctgc gtggagtaat c
//
LOCUS       DATLBZ010000060         5831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1536846, whole genome shotgun sequence.
ACCESSION   DATLBZ010000060 DATLBZ010000000
VERSION     DATLBZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536846"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VMZ53_03010"
     CDS             complement(<1..932)
                     /locus_tag="VMZ53_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27443.1"
                     /translation="MAALLVLLAICTALFGERIGINAGQGWDGETYIRWIAHFKIHLI
                     DRGTDAYHAQRVLPSGVLHLVMRALHLRGTVPQMIALYYWFDAVMLVVAAVLWSHLGE
                     VMRWRAVSVWVGFAGMFGSFALARHALYNPALTDAAAFTLGMAVAWAYLARKPLALWL
                     CAGLAMFTWPALVPVAAACLLLPRLDGPLATPRSIRRVRLASIAIAFTLSAAFVLVAR
                     HYLAHPDTALGVPKFATWVRRDWLWITAPLVAMMLVVGWFTLLYQPQLWNVRAYLRTR
                     TARSFVIPLAGLAALFVLRHLWMRVAVTQHDGPTL"
     gene            complement(956..2539)
                     /locus_tag="VMZ53_03015"
     CDS             complement(956..2539)
                     /locus_tag="VMZ53_03015"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HUS27444.1"
                     /translation="MTPRVQPRRRRPPPGGWRLWLVPMGILGGIALLAFAGVYLLDDK
                     VTGGAGGTAPTGLTRYVHFEPGLISDATAGLAAMTAAVLGIVITVVSLLVQLTSARYT
                     GVAQMFLRDRTNVVVMAFYVVTCVVGVALSLSLHSEFVPRAAVMVMMCACALGLVIML
                     PYFAYVFRFLEPTNLIARIESEAAADVRKASMTRDPDTIAHAQGPALSALEELTDITS
                     NSISGRDKIIASRAVDAIKDFVVGYLAIKNKADERWFNVGEGIRDNPDFVAMDPESLR
                     VLEREHTWLEWKALRQYLGIYAEAAQGMPDIQYLIAIDTRYVAEAALAHNDRNVVELA
                     FRFMNSYLRAALNARAVRTAYNVMNQYRLLVEAMIRGGADDAAIEGVRHMIYYGRTSY
                     DMQLGFVTETVAYDVSTLCELAHHQKRQVDARLLEMFLDLDQPIRLKKQETGLQGIRK
                     AQLKLACYYLSVGAEERAKKIATDMATEDRARLQSIREQLSKVESKEFWEVTDRGGNF
                     EYMPPKQKEQMDRFFGMLP"
     gene            2598..3167
                     /locus_tag="VMZ53_03020"
     CDS             2598..3167
                     /locus_tag="VMZ53_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27445.1"
                     /translation="MSDHSSLEERGKALEDQFFDKQDKEKLAAMKTKLDSQASREDLR
                     KASGMTDDAVLDKLVGLGLKANTIAALSLVPLIEVAWADGEIQDNERTAILQGAHGKG
                     LEQGTDGYELLQSWLKKKPSGDLLDAWEAYIKALASQLNDEQNRLLKNQIVGFAKMVA
                     AAAGGFLGIGRVSASEEKVLARIETAFAR"
     gene            3170..4156
                     /locus_tag="VMZ53_03025"
     CDS             3170..4156
                     /locus_tag="VMZ53_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUS27446.1"
                     /translation="MRFLGKTGVKVSRVALGTMSFGGDADEATAGEIWRAARAAGINL
                     IDSADVYNEGRSEEITGRLMKGERDELVLATKAYFPTGKDVNARGSSRYHLVRAVEGS
                     LRRLQTDRIDLFYLHRFDDVTDLGETLRAIEDLVRSGKILYPAVSNFAAWQVAHALGV
                     AAASGFAPLVAVQPMYNLVKRTAEIELLPMAHSLNVAVIPYSPTGGGLLTGKYGPDKK
                     PDKGRLLDTKMYTTRYANPEFYAIAGEFTKLAAELGHAPTTLAVAWVKSHPAVTSVLV
                     GGRNVEQLAPSFAALNVELDAETRARISALSPEPPPATDRNEEKTANNYGTR"
     gene            complement(4153..4686)
                     /locus_tag="VMZ53_03030"
     CDS             complement(4153..4686)
                     /locus_tag="VMZ53_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27447.1"
                     /translation="MSLIVVINALLAIVGGILATSAIIVAKRPDAKQMIDKLAPYQVL
                     IGVALVAVGIVDFVKNLKVFTDLMKVNYFSAGAMLALIASSVLLGLLFGAPTFLKLVS
                     GGPPPPPTNPYAQQYQQMMMQQGQMPPNRAEQRLLELTQKIAPFQVLIGIVALGSALI
                     VLLYQFKILKYAGGGPI"
     gene            complement(4721..>5831)
                     /locus_tag="VMZ53_03035"
     CDS             complement(4721..>5831)
                     /locus_tag="VMZ53_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HUS27448.1"
                     /translation="EAGLVLAPHTRWHTGVTFSGAMIVDLVHDIARRAETLEDAIKIA
                     RERPVSSTWGIAVGSAREKSALVLEIAGPHVEVVRPAPGASFLVCANRYRTPALQAGQ
                     VAASSAWALHSERRERRLRQLVETRESPLTAEMLVRFMGDRQDVDAPGIKRHLGGIPA
                     QPTNVHCAVVSPSKLRAYVGVDRAPTCEGTWAELSWTWDGPTGGWDSSELASPPAGAA
                     ASAAGFDVRVRDDLAAPQDEATRHIHDAARAYESRHDVRAARAAIERAVAVAPDDPSL
                     RLTAAWLALEEGIADRAVIHVHAGLAKERDTYRRGQLLLWGSRAARTIDPALSTRWTD
                     ELARLAGPDVEELKTASRKKYRGKPHVNFMMADAY"
BASE COUNT         1070 a         1898 c         1941 g          922 t
ORIGIN      
        1 agcgtcgggc catcgtgctg cgtgaccgcg acgcgcatcc agaggtggcg cagcacgaag
       61 agggccgcga gtccggcgag cgggatgacg aacgaccgcg cggtccgcgt ccggaggtag
      121 gcgcgcacgt tccagagctg cggttgatag agcagcgtga accagccgac cacgagcatc
      181 attgcgacga gcggcgccgt gatccagagc cagtcgcgcc gcacccatgt cgcaaacttc
      241 ggcacgccaa gcgccgtgtc cggatgcgcg aggtagtgac gagcgacgag cacgaacgcc
      301 gcgctcagcg tgaaggcgat cgcgatggat gcgagccgga cgcggcggat cgaacgcggc
      361 gtggcgagcg gtccgtcgag ccgaggcagc agcaggcacg cagctgcgac ggggacgagc
      421 gcgggccacg tgaacatcgc gaggccggca cagagccaga gcgctagcgg cttgcgagcg
      481 agatacgccc aggcgacggc catcccgagc gtgaacgcgg cggcatcggt cagcgccggg
      541 ttgtacagcg catgacgcgc gagcgcgaac gacccgaaca ttcctgcgaa gccgacccag
      601 acagagacgg cgcgccagcg catcacctcg cccaggtgcg accacagcac ggcagccacg
      661 acgagcatca ccgcgtcgaa ccagtagtac agcgcgatca tctgcggcac cgttccgcgg
      721 aggtgcagcg cgcgcatcac gaggtgcagc acgcccgatg ggaggacgcg ttgcgcgtga
      781 tacgcgtccg tgccgcgatc gatcaggtgg atcttgaagt gcgcgatcca gcggatgtac
      841 gtctcgccgt cccagccctg ccctgcattg atgccgatgc gctcgccgaa tagtgcggtg
      901 cagatcgcga ggaggacgag cagcgcggcc atcagcaccg cgcgcagtcg ccagctcagg
      961 gcagcatccc gaagaagcga tccatctgct ccttctgctt gggcggcatg tactcgaagt
     1021 tgccgccgcg gtccgtgact tcccagaatt ccttcgactc gaccttcgag agctgctcgc
     1081 gaatcgattg cagacgcgcg cggtcttcgg tggccatgtc tgtcgcgatc tttttggcgc
     1141 gctcttcggc gccgaccgag aggtagtagc aagcgagctt gagctgcgcc ttgcggatgc
     1201 cttgcaggcc ggtctcctgc ttcttgaggc gaatcggctg atcgagatcg aggaacatct
     1261 cgagcaggcg cgcgtcgact tgccgcttct ggtggtgcgc gagctcgcag agcgttgaca
     1321 cgtcgtacgc gacggtttcg gtgacgaagc cgagctgcat gtcgtagctc gtgcggccgt
     1381 aatagatcat gtgacgaacg ccttcgatcg ccgcgtcgtc ggcgccgccg cgaatcatcg
     1441 cttcgacgag gagccgatac tgattcatca cgttgtacgc ggtgcgcacg gcgcgtgcgt
     1501 tgagggcggc gcgcagatag ctattcatga agcgaaacgc gagctcgacg acgttgcggt
     1561 cgttgtgcgc gagcgcggct tcggcgacgt agcgcgtgtc gatcgcgatc aggtattgga
     1621 tgtccggcat gccttgcgcc gcttcggcgt agatgccgag gtattggcgc agcgccttcc
     1681 actcgagcca cgtgtgctca cgctcgagca cgcgcagtga ctcgggatcc atcgcgacga
     1741 agtcgggatt gtcgcgaatg ccttcaccga cgttgaacca acgttcgtcg gccttgttct
     1801 tgatcgcgag atagccgacg acgaaatctt tgatcgcgtc aaccgcgcgg ctcgcgatga
     1861 tcttgtcgcg accggagatc gagttcgacg tgatatcggt gagctcttcg agcgccgaga
     1921 gcgccggacc ctgcgcgtgc gcgatcgtat cgggatcgcg cgtcatcgat gctttgcgaa
     1981 cgtcggccgc ggcttcggac tcgatacgcg cgatcaggtt cgtcggctcg aggaaccgga
     2041 acacgtacgc gaagtacggc agcatgatga cgagaccgag cgcgcacgcg cacatcatca
     2101 ccatcaccgc cgcgcgcggc acgaactccg aatggagcga gagcgagagc gcgacgccga
     2161 cgacacacgt cacgacgtag aacgccatca cgacgacgtt cgtgcgatcg cgcaagaaca
     2221 tctgggcgac gcccgtgtag cgcgcggacg tgagctgcac gaggagcgag acgacggtga
     2281 tgacgatacc gagcaccgca gcggtcatcg cggcgagacc tgcggtcgca tcggagatga
     2341 ggccgggctc gaagtgcacg tatcgggtga ggccggtggg ggctgtgcca cccgcgccgc
     2401 cggtcacttt gtcgtcgagg agatacacgc ccgcgaacgc gagtagtgca attccgccga
     2461 gaatccccat gggcacgagc cagaggcgcc aaccacccgg cggtggcctg cgtctacgtg
     2521 gctggaccct tggcgtcaca ggcagacgat acccgaaagt tttgataggg tcgcagctcc
     2581 caacaaggag tcgagctgtg agtgatcatt cctctctcga agaacgcggc aaagccctcg
     2641 aggatcagtt cttcgacaaa caggacaaag agaagctcgc cgcgatgaag acgaagctcg
     2701 acagccaagc ttctcgcgaa gacctccgca aggcgtccgg catgaccgac gacgccgtgc
     2761 tcgacaagct cgtcggcctc ggcctcaagg cgaacacgat tgccgcgctg tcgctcgtcc
     2821 cgctcatcga agtggcgtgg gccgacggcg agatccagga caacgagcgc accgcgatcc
     2881 tgcaaggcgc gcacggcaag ggcctcgagc aaggcaccga cggctacgag ctgctccagt
     2941 cgtggctcaa gaagaagccg tcgggcgacc tgctcgacgc gtgggaggcc tacatcaagg
     3001 cactcgcgag ccagctcaac gacgagcaga accgcctgct gaagaaccag atcgtcggct
     3061 tcgcgaagat ggtcgcggcc gcagcgggtg gcttcctcgg tatcggtcgc gtgtcggcgt
     3121 ccgaggagaa agtcctcgcg cgcatcgaaa ccgcgttcgc gcgctagtca tgcgcttcct
     3181 cggcaagacc ggggtgaagg tgtcgcgcgt cgcgctcggc acgatgtcgt tcggcggtga
     3241 cgcagacgaa gcgaccgccg gcgaaatctg gcgagccgcg cgtgccgccg gcatcaacct
     3301 catcgatagc gccgacgtct acaacgaagg ccgctccgag gaaatcacgg gtcgcctcat
     3361 gaagggcgag cgtgacgagc tcgtgctcgc gacgaaggcg tatttcccga cgggaaaaga
     3421 cgtcaacgcg cgtggctcgt cgcgctatca cctcgtgcgc gccgtcgaag gctcgctccg
     3481 gcggctgcag accgatcgca tcgacctgtt ctatctgcat cgcttcgacg acgtgacgga
     3541 cctcggcgag acactgcgcg ccatcgagga tctcgttcgc agcggcaaga ttctctatcc
     3601 cgccgtgtcg aacttcgcgg catggcaagt cgcgcatgcg ctcggcgtcg cggcagcgtc
     3661 gggcttcgcg ccgctcgtcg cggtgcagcc gatgtacaac ctcgtgaagc gcacggccga
     3721 gatcgagctt ctgccgatgg cgcactcgct caacgtcgcg gtgattccgt attcaccgac
     3781 gggcggcggt ctgctcaccg gcaagtacgg ccccgacaaa aagcccgaca aagggcgcct
     3841 gctcgacacg aagatgtaca cgacgcgcta cgcgaacccg gagttctacg cgatcgcggg
     3901 tgaattcacg aagctcgcgg ccgagctcgg ccatgcaccg acgacgctgg ccgtcgcgtg
     3961 ggtcaagtcg catcccgcgg tcacgagcgt cctcgtcggt ggacgcaacg tcgaacagct
     4021 cgcgccatcg ttcgctgcac tgaacgtcga gctcgatgcc gagacacgtg cgcgaatcag
     4081 cgctctctct cccgagccgc ctcccgctac cgatcgcaac gaagagaaga cggcgaacaa
     4141 ctacggcacg cgctagatcg gtccgccgcc cgcgtacttc agaatcttga actgatagag
     4201 cagcacgatc agcgccgagc cgagcgcgac gatgccgatc aggacctgga acggcgcgat
     4261 cttttgcgtc agctcgagga ggcgctgctc cgcacggttc ggcggcatct ggccttgctg
     4321 catcatcatc tgttggtact gctgcgcgta cggattcgtc ggtggcggtg gaggaccgcc
     4381 cgagacgagc ttcaagaacg tcggtgcgcc gaacaacagg ccgaggagca ccgagctcgc
     4441 aatgagcgcg agcatcgcgc cggccgagaa gtagttcacc ttcatcaggt cggtgaagac
     4501 cttcaggttc ttcacgaagt cgacaatacc gaccgcgacg agtgccacgc cgatcagcac
     4561 ttgatagggc gcgagcttgt cgatcatttg cttcgcgtct gggcgcttgg cgacgatgat
     4621 ggcggacgtg gcgagaatgc cgccgacgat ggcgaggagc gcgttgatca cgacgatgag
     4681 gctcatgatg tttctccgca gaacatcgta cctcagctgc tcagtacgcg tcggccatca
     4741 tgaagttgac gtgcggcttg ccgcggtact tcttgcgcga agccgtcttg agctcctcga
     4801 cgtcggggcc cgccagccgc gcgagctcgt cggtccatcg cgtggagagc gccggatcga
     4861 tcgtgcgtgc cgcgcgcgag ccccacagca ggagctgacc gcgccggtac gtgtccctct
     4921 ccttcgcgag gcccgcgtgg acgtggatca ccgcgcgatc ggcgatgccc tcctcgagtg
     4981 cgagccacgc ggcggtgagg cgcagcgagg ggtcgtcggg cgcgacggcg acggcgcgtt
     5041 cgatcgcagc gcgtgcagcg cgcacgtcgt gccgcgactc gtacgcgcgt gccgcgtcat
     5101 gaatgtggcg agttgcttcg tcctgtggcg cggccaggtc gtcgcgaaca cgcacgtcga
     5161 accccgcagc cgacgccgct gcgcccgctg gaggcgacgc tagctccgag ctatcccagc
     5221 cgccggtcgg tccgtcccac gtccacgaga gctccgccca tgtgccttcg cacgtcggcg
     5281 cacgatccac accaacgtac gcacgcagct tgctaggcga gacgaccgca caatgcacgt
     5341 tcgtcggctg cgccggaatg ccgccgaggt gacgcttgat gcccggcgca tcgacgtcct
     5401 ggcgatcgcc catgaacctg acgagcatct ccgcggtcag tggcgactcg cgcgtctcga
     5461 cgagctggcg caacctgcgc tcgcgacgct ccgaatgcag cgcccacgcc gacgacgcgg
     5521 cgacctgccc tgcttgcagc gcgggcgtgc gatagcgatt cgcgcacacg aggaagctcg
     5581 cgcccggcgc tggccgaacc acttcgacgt gcggtcccgc gatctcgagc acgagtgcgc
     5641 tcttctcgcg agcggagccc accgcgatgc cccacgtgga cgacacggga cgctcacgcg
     5701 cgatcttgat cgcgtcctcg agcgtctcgg cgcggcgcgc gatatcatgc acgaggtcga
     5761 cgatcatcgc gccgctgaac gtcacgcccg tgtgccaccg cgtgtgtggc gcgagcacga
     5821 ggcccgcttc g
//
LOCUS       DATLBZ010000061         4465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1537446, whole genome shotgun sequence.
ACCESSION   DATLBZ010000061 DATLBZ010000000
VERSION     DATLBZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537446"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VMZ53_03040"
     CDS             complement(<1..458)
                     /locus_tag="VMZ53_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27449.1"
                     /translation="MAKTRTVTETERDRFGNVLASLDRADEVIASDDVYAEVRYSNDS
                     AHWILGRTTSIVVRAGSPSGPVLRSRSGDYDAQGELVAIHVDTGTGVATSTLGYDTFG
                     NLVHVETPPNETGQSQTYDVTFDPEIATYPIATRDGFGYTSTAQYDLRYGA"
     gene            1243..2124
                     /locus_tag="VMZ53_03045"
     CDS             1243..2124
                     /locus_tag="VMZ53_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HUS27450.1"
                     /translation="MEIDLHRLELRHRDLRIVDVDRRRRLIGSIAEIGQQVPAVVIAD
                     SDRFVLIDGYLRVEALTKLQRDTVLATTWSVSEVEALLHHRHLAITRRSALEDAWLLA
                     RLRDHGLTMDELARRLCRSKSWVSHRLGLLDALAVAVHDRVRAGTVPPHGAMRYLVPF
                     ARANRRQCEQLLARLGDARVSDRDLAALYTGWKRADREGKQRIVDAPLLFLQALASSK
                     TTAPVDDEASLLLKDLTMLAAIAGRANRRVREAGPWTAALTRAFGAASEAFAALGATI
                     EEMHARPDHPTSHPDPS"
     gene            2081..3562
                     /locus_tag="VMZ53_03050"
     CDS             2081..3562
                     /locus_tag="VMZ53_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27451.1"
                     /translation="MLDQTTRQAILTLHERGRGKRAIAKALGISRTTVRDVLASNAAT
                     PPPFERAERAESHHDEILTLHADCKGNLVRVHEELVARHADLALSYQALTAFCRKHGI
                     GHEAPKPAGRYEFEPGQEMQHDTSPHDAHIGGALRRVQTASLVLAYSRRLFFQFYPRF
                     TRFECKSFLDDGVEYDDGACKHCMIDNTHVVVLSGTGKQMVPVPEMEAFARRLGFTFI
                     AHEKGDANRSARVEGPFWYIERNFLAGRKFADFADANRQARAWCDQVNAKFRRSLSAS
                     SNQLYVVDRAAMRRLPAWRPEIYQLHQRVVDLEGYIHVEGHMYSVPYQLIGKRVEVRE
                     GKDTIRVFIGPREVALHDKIVSTLRNQRRTIATHRPPRGQSAAPVASIPEERELANAG
                     APFAEYAVTLKKRAGLRWPLALRRLAQMRRDYPTTPLSAAIQTAAHYGLFDLDRLERM
                     ILKNVATAYFVVPADRDEPPLDDEADDADGDDTDDAAEVSDEG"
     gene            3833..4102
                     /locus_tag="VMZ53_03055"
     CDS             3833..4102
                     /locus_tag="VMZ53_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS27452.1"
                     /translation="MPKAENIVFVGGTGVGKTGLASALLLKALQNGHRGVFMRAQDLF
                     DEMYASIADRSTRRLLNRLAKVDVLVIDEMGYLNLRTEQTNIFSS"
     gene            4102..4275
                     /locus_tag="VMZ53_03060"
     CDS             4102..4275
                     /locus_tag="VMZ53_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS27453.1"
                     /translation="MEERYRQRATIITTNLDYSEWANFLGNKALVDALLSRLRHQCHT
                     VKIDGPSLRDPSG"
BASE COUNT          791 a         1500 c         1434 g          740 t
ORIGIN      
        1 gcgccgtagc gaaggtcgta ctgtgcggtc gacgtgtagc cgaagccatc gcgcgtcgcg
       61 atcggatacg tcgcgatctc cggatcgaac gtgacgtcgt aggtctgcga ctgcccggtc
      121 tcgttcggcg gcgtctcgac gtgcacgagg ttaccgaacg tgtcgtagcc gagcgtgctc
      181 gtcgcgacgc cggtgccggt gtcgacgtgg atggccacga gctcgccctg cgcgtcgtag
      241 tcaccgctgc gactgcgcaa caccggcccg gacggggagc ccgcgcggac gacgatcgac
      301 gtcgtacgac cgaggatcca gtgcgccgag tcgttggagt agcgcacctc cgcgtagaca
      361 tcgtcggacg cgatcacctc gtctgcgcga tcgagcgagg cgagcacgtt gccgaatcga
      421 tcgcgctccg tttcggtcac cgttcgcgtc ttcgccacgg tgccgccctc ggcgcgcgtg
      481 tcttcatcct tcagatcaac tgcacgcgcg gcgcgagatc ttgcggatct cggagcaagg
      541 ctgcttcgag ctcttcctcg gagagcttcg agcgctccct cgcaacgggt cccaatcgat
      601 cggccccgcc attgccacag cgtgagcgcc gagcggacgg accgcaaggc tcgcggcccc
      661 gaccgggagt ttcgttgttc aagaatcgaa tccgtgacca actgtaccgc tcgtgaaaaa
      721 tgacccgggt gtgctcgcga aaattgaccc accgtcgggt tgatcgaggt cgagcgatcc
      781 tagcggcgct gtgcgaaaga agcgcccttg ctccgtgtgc cgccgttggt tcatgccaga
      841 tggccgcgta ggcgatcgac agaagacctg cggatcggtc gagtgcaagc gagcgcgcca
      901 cgagcgcgcg taccggtcgt ggcgagcggc gcatcgcgac tacgatcgag cgcgacgttg
      961 ggagcaggcg atcgagacgg cgaaggacga tccgaaaacg gccccgccag cgtcgaatgc
     1021 accgccgccg acgtccggat tgccctggga cgtcgtgcaa aacgagatgc gggtcgaagg
     1081 ccgcggtaat tcttgcggga gtagtccaag taatcggtgc gtacgtgcaa aaacgagatg
     1141 cgcaagcaag tcgttgaaat tgcagcggga gtagtccgac atgacccgga tcccgcgcaa
     1201 aacgagatgg gagcaatcgc atgacgccgg cagtgtgggg aggtggagat cgatctgcac
     1261 cggctcgagc tgcgccatcg cgatctgcgg atcgtggacg ttgatcgacg acgccgactg
     1321 atcggatcga tcgccgagat cggtcagcag gtgccggcag tagtgatcgc cgacagcgat
     1381 cggttcgtgc tgatcgacgg ctatctgcgc gtcgaggcgc tcaccaagtt gcagcgcgac
     1441 accgtgctcg cgacgacgtg gtcggtgtcc gaggtcgagg cgctgttgca ccataggcac
     1501 ctcgcgatca cgcggcgcag tgcactcgag gatgcgtggc tgctcgcgcg gctccgcgac
     1561 cacgggctga cgatggacga gctcgcgcgg cggctgtgtc gctccaagag ctgggtgtcg
     1621 catcggctcg ggctgctcga cgcgctcgcc gtcgccgtcc acgatcgcgt gcgcgcgggg
     1681 accgtgccgc cacacggcgc gatgcggtac ttggtgccgt ttgcgcgcgc aaacaggcgt
     1741 cagtgcgagc agctgctcgc acgcctgggt gacgcccgcg tctcggatcg tgatcttgca
     1801 gcgctctaca cagggtggaa gcgcgctgac cgcgagggca agcagcgcat cgtcgacgcg
     1861 ccgctgttgt tcttgcaggc gctcgcgtcg tcaaagacca cggcgcccgt cgacgacgag
     1921 gcgagcctgt tgctcaagga tctgacgatg ctcgccgcga ttgcggggcg agccaatcgt
     1981 cgcgttcgcg aggctggtcc atggaccgcc gcgctcaccc gcgctttcgg cgccgcgagc
     2041 gaggcgttcg cggcgctcgg cgccaccatc gaggagatgc atgctcgacc agaccacccg
     2101 acaagccatc ctgacccttc atgagcgagg acgcggcaag cgcgcgatcg ccaaggcgct
     2161 cggcatctcg cgcaccacgg tgcgcgatgt gctcgcgtcc aacgcggcga cgccgccgcc
     2221 atttgagcgc gccgagcgcg cggagtcgca ccacgacgag atcctgacgc tccacgccga
     2281 ctgcaagggc aatctggtgc gcgtgcacga ggagctggtc gcacgccacg cggatctcgc
     2341 gctcagttac caggcactga ccgcgttctg ccgcaagcac ggcatcggcc acgaggcgcc
     2401 gaagcccgca ggccgctacg agtttgagcc cgggcaggag atgcaacacg acacctcgcc
     2461 gcacgacgcc cacatcggcg gcgcgctacg ccgcgtgcag accgcgtcgc tcgtgctggc
     2521 gtactcgcgt cggctcttct tccagttcta cccgcggttc acgcggttcg agtgcaagtc
     2581 gttcctcgac gacggcgtcg aatatgacga cggcgcgtgc aagcactgca tgatcgacaa
     2641 tacccacgtc gtggtgctct cgggcaccgg caaacaaatg gtgccggtac ccgagatgga
     2701 ggcgttcgct cggaggctcg ggttcacgtt catcgcacac gagaaaggcg acgcgaatcg
     2761 ctcggcacgc gtcgaggggc cgttctggta catcgagcgc aattttcttg ccggccgcaa
     2821 gttcgccgac ttcgccgacg ccaatcggca agcgcgtgcc tggtgcgacc aggtcaatgc
     2881 caagttccgc cgcagcctgt ccgctagctc caatcagctc tacgtcgtcg accgcgccgc
     2941 gatgcgcagg ttgccggcgt ggcggcccga gatctatcag cttcaccagc gcgtcgtcga
     3001 tctcgagggc tacatccacg tcgaaggcca catgtactcg gtgccctacc agctgatcgg
     3061 caaacgcgtc gaggttcgcg agggcaagga cacgattcgc gtgttcatcg gtccgcgcga
     3121 ggtcgccttg cacgacaaga tcgtctcgac gctccgcaat cagcgccgca cgatcgccac
     3181 gcatcgtccg ccgcgcggac agtctgccgc ccccgtcgcg tcgatccccg aggagcgcga
     3241 gctcgctaac gctggcgcgc cgttcgccga gtatgccgtt acgctcaaga aacgcgccgg
     3301 gctccgctgg ccgctcgcgc tccgacggct cgcgcagatg cggcgcgact atcccacgac
     3361 accattgtcc gccgcgatcc agaccgccgc ccactacggc ctcttcgacc tcgatcgcct
     3421 cgagcggatg atcttgaaga atgtcgccac ggcgtacttc gtcgtccccg cggaccgcga
     3481 cgagccaccg cttgatgacg aagccgacga tgccgacggc gacgacaccg atgacgccgc
     3541 cgaggtgtcc gatgaaggat gacctcgagc agctgctcaa ggtcctccac ttgcgcaaga
     3601 tcgccgagct gttcgacgag gaggtcaagc gcgcggagaa gggcgaccca gctaccagga
     3661 gttcatagcg cgcctgctcc gcgctcagtg gcaggccaat caagaggctg cgctcacctg
     3721 gcgcatcaag cgcgccgggc tacccgaaca gtggacgctc gagaccttcc cgttcaagcg
     3781 gcagccaggc gtcaatcagc gccagattcg caccttcgcc gagctcgaat tcgtgcccaa
     3841 ggccgagaac atcgtcttcg tcgggggcac tggcgtcggc aagaccgggc tcgcgtccgc
     3901 gcttttgctc aaggccctcc aaaacggaca ccgcggcgtg ttcatgcgcg cgcaggatct
     3961 attcgacgag atgtacgcgt ccattgcgga ccgctcgacg cgacgcctgc tcaaccgact
     4021 cgccaaggtc gacgtcctcg tcattgacga aatgggctac ctcaaccttc gcaccgagca
     4081 gaccaacatc ttttcaagct gatggaggag cgctaccgcc agcgagccac catcatcacg
     4141 accaacctcg actacagcga gtgggccaac tttctcggca acaaggcgct cgtcgatgcg
     4201 ctgctcagcc gccttcgcca ccagtgccac accgtcaaga tcgacggacc ctcgttacgt
     4261 gacccctcgg gctgacctct cccgtcgcca tcacggactt gacgccctcg tcgctgcccc
     4321 gccgagggcg ttgtgctttc tgtgggctcg ctgctgcaac gaccaccggt cagtcacgat
     4381 ggaaatcagt tcgctgatcc agaatcgctc gatgggtcag ttctcacgag cacacctggg
     4441 tcagttctga cgagcgctga agcgc
//
LOCUS       DATLBZ010000062         2981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6731873, whole genome shotgun sequence.
ACCESSION   DATLBZ010000062 DATLBZ010000000
VERSION     DATLBZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6731873"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VMZ53_03065"
     CDS             <1..211
                     /locus_tag="VMZ53_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27454.1"
                     /translation="PVVDGAKVISTARTELALTSLPIIAVSAGGDSARKSALGAGANI
                     FLDKPMRLRQVIETIQKLITSAATP"
     gene            complement(212..1903)
                     /locus_tag="VMZ53_03070"
     CDS             complement(212..1903)
                     /locus_tag="VMZ53_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27455.1"
                     /translation="MRLVCAAALVAAVGCSKSPSDETLPPEPDPKGWTITVDMSGLDR
                     FVQPGDSTSWPVGGVATATEGLATVKVNDALVDVDASGAFVADVTVAPGLTRVPILAT
                     DDAGHERKADRTLLAAQFLADGDYNGSAAGLVLTDAILGAMSESIAGYASDVNIAAEI
                     LSRDVLSQDDRCVTWPVQARQGTVTAALVEDAGNLWLHIRVPNLYVYFEGSCQGLISQ
                     IPIAGQMGGTIDVWTRLTAKPPVNGDACLSAFDHTRPQVSITGWGFDVWGTSGPLQAW
                     IVDLFAGNKSTEARNQLTTEVGGRADEMLGTKLADVSVYEKTSELDLLGRPLALDLCV
                     SGIEKTAQSTLVARIAARATGAGMREAPGAPQLAGAVVRPAANELVLDGNLIGQLLFA
                     SWRDNGLTRTAPDIDAGILQVLMPGLAKEFPDATTAQVWIEAELPPLVRVTPEAAADL
                     SVELGDLMIDVSIDGKRVLRFGAVLTLSLDLTPMNGALVPAVVDTKATVALLDERHDG
                     PDAALEQAVQVQIGSAASKLLGDGAAIALPSLPGLGAPVAVTPDAGGRYLRIKLQ"
     gene            complement(1926..2423)
                     /locus_tag="VMZ53_03075"
     CDS             complement(1926..2423)
                     /locus_tag="VMZ53_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27456.1"
                     /translation="MERRDFLWMFGSGVVACTLGCERQEGVPGVDASADDAAATTPSA
                     DDASSAGSQDLDACIQNIIKLHDTYAQALYLDGTNGPLTGVITTAHVIAGSIVTMDFW
                     HGHNGMQHRFTLTPQHFADLKHGKRITVGTTTVDGHAHTLFVDPKDEEYRVAGAPDVD
                     VPIGC"
     gene            complement(2425..>2981)
                     /locus_tag="VMZ53_03080"
     CDS             complement(2425..>2981)
                     /locus_tag="VMZ53_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27457.1"
                     /translation="DALDGFQVASTHFTGAFAARNAGRDVLPELTASDAELVHELLMT
                     TPFVSARGSAWKQAGGMYAWAAAASGPGQIVPKNYNAAAIEVSEASCDSCHRDAGRPF
                     KTWYDNILAYGELWGNDEIFTWHPFTLDRFVDGNGAVVNFNYDNRQIRPDFVAAALIT
                     AYSATNHPATFYKRIVRDWTDFAY"
BASE COUNT          494 a         1020 c          964 g          503 t
ORIGIN      
        1 gccggtcgtc gacggcgcga aggtcatctc gactgcacgc acggagctcg cactcacgag
       61 cctgccgatc atcgcggtct ccgccggcgg ggactccgcg cgtaagtctg cgctcggagc
      121 gggcgcgaac atcttcctcg ataagccgat gcgcctgcgt caggtcatcg agacgatcca
      181 gaagctgatc acctccgccg cgacgcccta gctattggag cttgatccgc aggtagcgcc
      241 cgccggcatc cggtgtcacg gccacaggtg cgccgagccc gggcagtgac ggcagcgcga
      301 tcgccgcacc gtcgccgagg agcttcgaag cggcagagcc gatctggact tgcacggctt
      361 gctcgagcgc ggcgtccgga ccgtcgtgac gctcgtccag cagcgcgacc gttgccttcg
      421 tatcgacgac cgccggcacg agcgcaccgt tcatcggcgt cagatcgagt gagagcgtga
      481 gcaccgcgcc gaaccgcagc acccgcttgc catcgatcga gacgtcgatc atgagatcgc
      541 cgagctcgac gctgaggtcg gcagccgctt ccggcgtgac gcgcacgagc ggtggcagct
      601 cggcttcgat ccacacctgc gccgtcgtcg cgtcggggaa ttccttcgcg aggccgggca
      661 tgagcacctg gagaattccc gcgtcgatgt cgggcgccgt gcgagtcaga ccgttgtcgc
      721 gccacgatgc gaacaggagc tgaccgatga ggtttccgtc gagcacgagc tcgttcgctg
      781 caggcctcac gactgcaccg gcaagctgcg gcgcgcccgg agcctcccgc attcctgcgc
      841 ccgttgcgcg cgccgcgatg cgagcgacga gcgtgctctg tgccgtcttc tcgatgccgc
      901 tcacgcacaa gtcgagcgcg agaggacgcc ccagcagatc cagctcgctc gtcttctcgt
      961 agaccgagac atcggcgagc ttcgtgccga gcatctcgtc cgcgcgcccg ccgacttcgg
     1021 tcgttagctg gttgcgcgcc tcggtcgact tgttgccggc gaacagatcg acgatccacg
     1081 cttgcagcgg gccactcgtg ccccacacgt cgaagcccca accggtgatc gagacttgcg
     1141 gccgcgtgtg atcgaacgca gacaggcacg cgtcgccatt caccggcggc ttcgcggtca
     1201 accgcgtcca cacgtcgatc gttccgccca tctggccggc gatcggaatc tgcgagatga
     1261 ggccctgaca cgagccctcg aagtacacgt agagattcgg cacgcgaatg tgcaaccaca
     1321 ggttgccggc atcctcgacg agagcggccg tcaccgtgcc ctgccgcgcc tgcacgggcc
     1381 acgtcacgca gcggtcgtcc tgcgagagca cgtcccgcga gaggatctcg gcggcgatgt
     1441 tcacgtccga cgcgtagccc gcgatgctct cgctcatcgc gcctagaatc gcgtcggtca
     1501 gcacgagccc cgcagcgctt ccgttgtaat cgccgtcggc gaggaactgc gccgcgagca
     1561 gcgtgcgatc ggctttgcgc tcgtgcccgg cgtcgtcggt agcgaggatc ggcacacggg
     1621 tcagccctgg cgcgaccgtg acgtccgcga cgaacgcgcc actcgcgtcg acgtcgacga
     1681 gcgcgtcgtt gacctttacg gtggcaaggc cctcggtcgc ggtcgcaacg ccaccgacgg
     1741 gccagctcgt cgagtcgccg ggctgcacga agcgatcgag cccggacatg tcgacggtga
     1801 tcgtccagcc cttcggatca ggctcgggcg gcagcgtctc gtcgctcggc gacttcgaac
     1861 atccaacagc ggcgacgagc gccgcagcac aaacgagacg catgttttcc cctcctgcgc
     1921 gaacgtcaac agccgatcgg tacgtccaca tcgggcgcac cggctactcg gtattcctca
     1981 tccttcggat cgacgaacag cgtgtgcgca tggccgtcga cggtcgtcgt gccgacggtg
     2041 atgcgtttgc cgtgcttgag atccgcgaag tgttgcggcg tcagcgtgaa gcgatgctgc
     2101 atgccgttgt gcccatgcca gaagtccatc gtcacgatgc ttcccgcgat cacgtgcgcg
     2161 gtcgtgatga cgccggtcag cgggccgttc gtaccgtcga ggtagagcgc ctgcgcgtac
     2221 gtgtcgtgca gcttgatgat gttctggatg cacgcgtcga ggtcctgcga gcccgcgctg
     2281 ctcgcgtcgt ccgccgacgg cgtcgtcgcc gctgcatcat ccgcgctcgc gtcgacgccc
     2341 ggcacgccct cttggcgctc gcagccgagc gtgcaggcga ccacgccgct gccgaacatc
     2401 cagagaaagt cgcggcgctc catgtcagta cgcgaagtcc gtccagtcac ggacgatccg
     2461 cttgtaaaag gtggccggat gattcgtcgc tgaatacgct gtgatgaggg cagcggcgac
     2521 gaagtccggc cgaatttgcc gattgtcgta gttgaagttg acgacagcgc cattcccgtc
     2581 gacgaaccgg tcgagcgtga atggatgcca cgtaaaaatc tcgtcgtttc cccagagctc
     2641 gccgtacgcg agaatgttgt cgtaccaagt cttgaacggg cgcccggcgt cgcgatgaca
     2701 cgagtcgcat gacgcctcgc tgacttcgat cgcagcagcg ttgtaattct tcggaacgat
     2761 ctggccgggt cccgacgcag ccgcagccca cgcatacatc ccaccggctt gcttccacgc
     2821 ggaaccgcgc gcggacacaa acggcgtcgt catgagcagc tcgtgcacga gctcggcgtc
     2881 gctcgcggtc agctcgggca gcacatcgcg gccggcattg cgcgcggcaa acgcgccggt
     2941 gaagtgcgtc gaggcgacct gaaatccgtc gagcgcgtcg c
//
LOCUS       DATLBZ010000063         6067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4519704, whole genome shotgun sequence.
ACCESSION   DATLBZ010000063 DATLBZ010000000
VERSION     DATLBZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4519704"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VMZ53_03085"
     CDS             complement(<1..392)
                     /locus_tag="VMZ53_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27458.1"
                     /translation="MANALPLEAADHALLEVGLQLREHGYSFITVTPETHRRNNARPD
                     AREARTLRDVFGWSRPFKPDVLPREMLDTLQRAGALVEEQGLMRSSVRFSTLGAGLYV
                     HSAYPTLDENAVFFGPDTYRFCGLVEGMA"
     gene            complement(410..1615)
                     /locus_tag="VMZ53_03090"
     CDS             complement(410..1615)
                     /locus_tag="VMZ53_03090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUS27459.1"
                     /translation="MLRLLIHALATIVFVNRMFGGLLLRALRGRTWDETTDDFEPTVT
                     VVIPMFNEGRAVVETLRSVLASDYPRDKLDVICVDDQSIDDTFDRACEVARTDPRLVV
                     VRNATNVGKRCSINHAVRRSRSEVIVSVDSDVIIDPDAIRQLVRRFTSPKIAAVGGWV
                     DVRNKDENWLTRMQVLKYWYAYYVVKNVERAYKTVLAVSGCLAAYRRSVLLELMPILD
                     DRNILGVPIKYGEDRFLTRQIVKAGYHTTLTNQARCRTFVPSTLFGYFSQQLRWRRSN
                     IVDFVGGFSHVWRLHPLVALNYFSMAIVLFLYPVGVARAAMAGLIVPAVLAHVVALFV
                     YGLYYRWRTRTWDPADRVGPLSYVPHALVMPISSGLLVAVALFTLDSTRWETRGAPRP
                     SAAPKPNPS"
     gene            1688..2791
                     /locus_tag="VMZ53_03095"
     CDS             1688..2791
                     /locus_tag="VMZ53_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS27460.1"
                     /translation="MKVLLVEEGWHATMAIARGLHDAGLHVTVNTANGTSARCEHDGV
                     EWRSGPPVRSDRFVPELDALVTARAIDVVLPLTEAAMCRLWDAGVTWSERIFPHTQRW
                     QQALVRDKHALLEHLAGNGVAVPRYQRVDDTFDPAAVIASLGLPLVVKGATGSGGRHV
                     RIASSESELVHALERAAAIDDRWIVQEHVDGPTYLVGGLFDRGRPLRLYAAEKLELHP
                     PRIGGAIRVRSVSDRALVAAGVRAIELLGWTGFASVDLMRRGDEYVVLEINPRLWGSF
                     AGATSAGVDLLAPFAQLLAGQTPAPVLEFAADSTCMIFPRYLNAAAHRNVAGIVQAWR
                     DLRGEQGRDWRDPRFVLHVLRRLYYMKQDAVRL"
     gene            complement(2802..3287)
                     /locus_tag="VMZ53_03100"
     CDS             complement(2802..3287)
                     /locus_tag="VMZ53_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27461.1"
                     /translation="MMRRSATFVVLLTLGACSQKDSKPAPAPEDKPPTRTVGSAGSAA
                     ASETPPPAPPPPSETDAAAEVADLVVHDVGSKALGSQKIEASCVSVVVMPGGKWTIAA
                     ARLNGCGDKTARSILWLYKRPETGGKWNEDYLGQPPKCWKGVPADILEAVTKSTKIPS
                     C"
     gene            complement(3284..5506)
                     /locus_tag="VMZ53_03105"
     CDS             complement(3284..5506)
                     /locus_tag="VMZ53_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27462.1"
                     /translation="MIRCAALRTSNHRPTTTIAVVAFAVLVLELTSTRLFAYIGSAHA
                     TSTALSIALLGLGIGAFLRLRFPAWTESRRVAIGLALAIGVLASITTLGVSLPGLVAV
                     SLVPFVLAGMLLSDAYAARGHEAARATYAVDLFSAACGCLVAPRLIGPLSPTEIMLAL
                     GLLCSGLALVLAERQRRYVAVVALIAVAQTALLVASKTGALPDGPLDVLVRSDPHSEK
                     AIVDTATSSRNVLDSAWSPLGRLDVHQRSDERGERLGVYTDGMSPTYMVEEQRAKAGS
                     FESTWGLLMSLPYRAFRPERVLVLGAGAGASVWLAKKYGAARVDAVEVNPAIPGVLER
                     WKRFAGDVYHQPGVRLFVEDARRHLIDTSERYDEIELALALTGNVHTQAAGMEAHLYT
                     VEAVRLYLSRLTPNGSLVLIHSDPGNAYRQLLTVIEALGIPRDKALEGIVVLYDPRPG
                     TAYRYMVVVRTSPMPLDAVDELDVPPAELLWGPGDPPERAAELLDPKKLGATANDDLT
                     PVHDERPYFFQNTKGLVATALAEPGRLWVLAGAVVLVLLLMFERGHRSLRPAAAAAGL
                     GAAFLCVELALIQRFTLAAGGTLYAVSIVLFGLLCWSGFGALVLGGRWRRLERGVSWA
                     CVAAAVAVAATAWIMRDLSWLDGISSEAGRLVVIMLMLGIAGVALGGPFPVLLAHHGE
                     PSDRIASLWAINGAASVAGGVIAVLALRVAGSAYALYLAAGLYLVTALLAGPRGRVDA
                     "
     gene            5655..>6067
                     /locus_tag="VMZ53_03110"
     CDS             5655..>6067
                     /locus_tag="VMZ53_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27463.1"
                     /translation="MQSSLAIALACVTLTACAGVVDETGSGDDGAGGEEMSRPVDIEG
                     PDVLPHVQRFADLVCGSLDACHISTYAGHHPVASRAIDILVSDVYGRVPSDDNALGDA
                     VAAFALAHQDPAGIMYVIWRQRYNDGSGWDPMEDRG"
BASE COUNT         1102 a         1974 c         2041 g          950 t
ORIGIN      
        1 gccatgcctt cgacgaggcc gcagaaccgg tacgtgtcgg ggccgaagaa gaccgcgttc
       61 tcgtcgagcg tcgggtaggc ggagtgaacg tagaggccgg cgccgagcgt cgagaaccgg
      121 accgacgagc gcatcaggcc ctgttcctcg acgagagcgc ccgcgcgctg caaggtgtcg
      181 agcatctctc gcgggagcac gtcgggcttg aacggccggc tccagccgaa gacatcgcgc
      241 agcgtccgcg cctcgcgcgc gtcggggcga gcgttgttgc gccgatgggt ttcgggcgtc
      301 accgtgatga acgagtaccc gtgctcgcgg agctgcaggc caacctcgag gagcgcatgg
      361 tcggcagctt cgagcggcag cgcgttggcc atgcgcgact ctagatcgcc tacgagggat
      421 ttggtttcgg cgcggcactg gggcgtggcg caccacgcgt ttcccagcgc gtcgaatcga
      481 gcgtgaacag cgcgacggcg acgaggaggc cactgctgat cggcatcacg agagcgtgtg
      541 ggacgtacga gagtggccct acgcgatcgg cagggtccca cgtgcgcgtg cgccagcgat
      601 agtagaggcc gtagacgaac agcgcgacga cgtgcgcgag caccgcaggc acgatcagac
      661 ccgccatcgc cgcgcgcgcg acgccgaccg ggtagagaaa cagcacgatc gccatcgaga
      721 aatagttgag cgcgacgagc ggatgcagcc gccacacgtg actgaagccg cccacaaagt
      781 cgacgatgtt cgagcgacgc cagcgaagct gctgcgagaa atagccgaac agcgtcgaag
      841 gcacgaaggt gcggcagcgc gcctgattcg tgagcgtcgt gtggtagccc gctttgacga
      901 tctgcctcgt gaggaagcgg tcctcgccat acttgatcgg aacgccgaga atgttccgat
      961 cgtcgaggat cggcatgagc tcgagcaaca ccgaacgccg atacgcggcg agacaacctg
     1021 acaccgcgag cacggtcttg tacgcgcgct cgacgttctt cacgacgtag tacgcgtacc
     1081 agtacttcag cacctgcata cgcgtcagcc agttctcgtc cttgttgcgc acatcgaccc
     1141 aaccgccgac cgccgcgatc ttcgggctcg tgaacctgcg caccagttgg cggatcgcgt
     1201 ccgggtcgat gatgacatcc gagtcgacgg acacgatcac ctcgctgcgc gaccggcgta
     1261 ccgcgtgatt gatcgagcag cgcttgccga cgttcgtcgc attgcgcacg acgacgagtc
     1321 gcggatcggt gcgcgccact tcgcacgcgc gatcgaaggt gtcatcgatc gattgatcgt
     1381 cgacgcagat cacgtcgagc ttgtcgcgcg gatagtccga tgcaagcacg ctgcgaagtg
     1441 tctcgacgac ggcccgcccc tcgttgaaca tcgggatcac gaccgtcacc gtcggctcga
     1501 agtcgtcggt cgtctcgtcc cacgttcggc cgcggagcgc gcgcaagagc agaccgccga
     1561 acatgcgatt cacaaacacg atcgtcgcca atgcgtgaat gagaagccgc agcacccaag
     1621 ctaacgatcg ggtccgcggc ttccgccgtc aaccacggat ttctgatgcc gtgtatcgta
     1681 gcggcgagtg aaggtgttgc tcgtcgaaga gggctggcac gcgacgatgg cgatcgcgcg
     1741 cggccttcac gatgcgggcc tccatgtgac ggtgaacacc gcgaatggaa cgtcggcacg
     1801 ttgcgagcac gacggtgtcg agtggcgttc aggcccaccg gtgcgcagcg atcgcttcgt
     1861 gcccgagctc gatgccctcg tcacagctcg cgcgatcgat gtcgtgctgc cgctcacgga
     1921 ggccgcgatg tgccggctct gggatgccgg cgtcacgtgg agcgagcgaa tctttccgca
     1981 tacgcaacgc tggcaacagg cgctcgtgcg agacaagcac gcgctgctcg agcacctcgc
     2041 cgggaacggt gtcgcggtgc cgagatacca gcgcgtcgac gacaccttcg atccggcagc
     2101 cgtgatcgca tcgctgggct tgccgctcgt cgtgaagggc gcgaccggct cgggtggaag
     2161 acacgtgcgt atcgcatcga gcgagtccga gctcgttcac gcgctcgagc gtgccgcggc
     2221 gatcgacgat cgatggatcg tgcaagagca tgtcgacggc ccgacgtatc tcgtcggtgg
     2281 cctcttcgac cgcggtcgac cacttcgtct atacgccgcc gaaaagctag agctccaccc
     2341 gccgcgcata ggcggcgcga tccgtgtgcg gtcggtgagc gatcgcgcgc tcgtcgcggc
     2401 cggggttcgc gcaatcgagc tactcggttg gacgggattc gcgagtgtcg atctgatgcg
     2461 gcgaggcgac gagtatgtcg tgctcgagat caacccgcgt ctgtggggct cgttcgcagg
     2521 cgcgacgtct gcaggtgtcg acctgctcgc tccattcgcg cagttgctcg caggtcagac
     2581 gccagcgccc gtcctcgagt tcgcggctga ctcgacgtgc atgatcttcc cgcgctactt
     2641 gaacgccgcg gcgcatcgca acgttgcggg catcgtgcag gcgtggcgcg atctgcgcgg
     2701 cgagcaaggt cgcgactggc gcgatccgcg cttcgtgctc cacgtccttc gccgcctcta
     2761 ctatatgaag caggacgcgg tgcgcttgtg acgtgatctg gttagcagct cgggatcttc
     2821 gtcgacttcg tcactgcttc gaggatgtcg gcaggtacgc ccttccagca tttgggcggc
     2881 tgacctagat aatcttcgtt ccacttgccg ccggtctcgg gacgcttgta gagccacaag
     2941 atcgagcggg cggtcttatc gccacagcca ttgagtcgcg ccgcggcgat agtccatttc
     3001 ccgcccggca tgaccacgac cgagacgcag ctcgcttcga tcttctggct gccgagcgct
     3061 ttcgatccga catcgtggac gacgaggtct gctacctcgg cagctgcatc ggtctcggag
     3121 ggaggcggcg gagccggcgg tggtgtctcg ctcgccgcgg cggagcccgc cgaaccgaca
     3181 gtacgcgtcg gaggtttgtc ctctggtgcg ggtgcaggtt tcgaatcctt ctgcgagcac
     3241 gcacccaacg tgagcagcac cacgaacgtc gccgacctgc gcatcatgcg tcgactctac
     3301 cgcgaggacc cgcgagcaac gccgtcacga gatacaaacc ggcggcgaga tacagcgcgt
     3361 aggccgaacc ggcgacacgc agcgcgagca cagcaatgac gccgccggcg accgacgctg
     3421 cgccgttgat cgcccacaag ctcgcgatgc gatctgatgg ctcgccatga tgagcgagca
     3481 gcacgggaaa cgggccgccg agcgcaacgc ccgcgattcc gagcatcagc atgatgacga
     3541 cgagacgacc cgcttcgctc gaaattccgt cgagccacga gaggtcgcgc atgatccatg
     3601 cggttgccgc aactgcgacc gccgccgcga cacacgccca tgacacaccg cgctcgagcc
     3661 gacgccaccg accaccgagg acgagcgccc cgaacccgct ccagcagagg agcccgaaca
     3721 gcacgatcga cactgcgtag agggtgccgc ccgccgcgag cgtgaaacgc tggatcagcg
     3781 cgagctcgac gcataggaag gcggcgccga gaccagcggc agcggcagcg ggacgaagcg
     3841 agcgatggcc acgctcgaac atcaggagca gcacgagcac gaccgcaccg gcgagcaccc
     3901 agaggcgacc gggctccgcg agcgccgtcg cgacgaggcc cttcgtgttc tggaagaagt
     3961 atggacgctc gtcgtgcacc ggcgtcaggt cgtcattcgc ggtggcgccg agcttcttcg
     4021 gatcgaggag ctcggcggca cgctcgggcg gatcgccggg tccccacagc agctcggcag
     4081 gcggaacgtc gagctcgtca acggcatcga gcggcatcgg cgaggtgcga acgaccacca
     4141 tgtagcgata cgccgtgccc ggccgcggat cgtagagcac gacaattccc tcgagtgcct
     4201 tgtcgcgcgg gatgccgagc gcttcgatca ccgtgagcag ctgccggtat gcgttgcccg
     4261 ggtcgctgtg gatgagcacg agcgagccgt tcggcgtcaa ccggctgaga tagagacgca
     4321 ccgcttcgac cgtgtagaga tgcgcctcca taccagctgc ttgcgtgtgg acgtttcccg
     4381 tcagcgctag cgcgagctcg atctcgtcgt agcgctcgct cgtatcgatg agatgtcgac
     4441 gcgcatcctc gacgaagagg cgcacgccgg gctggtgata cacgtcgccg gcgaagcgct
     4501 tccaccgttc gagcacgccc gggatcgctg gattgacctc gaccgcatcg acgcgcgccg
     4561 cgccgtactt cttcgcgagc cacacgctgg cacccgcgcc cgcgccgagc acgagcacgc
     4621 gctctggacg aaacgcgcga tacggaagcg acatgagcaa gccccacgtg ctctcgaacg
     4681 agccagcctt cgcgcgttgc tcttcgacca tgtacgtcgg gctcatgccg tcggtgtaga
     4741 cgccgagtcg ttcgccgcgc tcgtcgctgc gttgatgaac gtcgaggcga ccgagtgggc
     4801 tccacgcaga atcgagaacg ttgcgcgatg acgtcgcggt atcgacgatc gctttctccg
     4861 agtgcgggtc ggaccgaacg agcacgtcga gtgggccatc aggcagcgcg ccggtcttcg
     4921 aggcgacgag caacgcggtt tgcgctacgg cgatcagcgc gacgaccgca acgtagcgac
     4981 gctggcgctc agcgaggacg agcgcgaggc cggagcaaag caggccgagc gcgagcatga
     5041 tttccgtcgg agagaggggg ccgatgagcc gcggcgcaac gagacagcca cacgcggcgg
     5101 agaacagatc gacggcgtac gtcgcgcgcg cggcttcgtg accgcgggcc gcgtacgcat
     5161 ccgagagcaa catgccagcg agcacgaatg gcacgaggga cactgcaacg agccctggaa
     5221 gcgaaacgcc cagcgttgtg atcgacgcca ggacaccaat cgcgagcgcc agcccgatcg
     5281 caacacgccg agactcggtc catgcgggga atcgcaagcg gaggaacgcg ccgatgccga
     5341 ggccgagaag cgcgatcgag agcgccgtcg acgttgcgtg cgcgctaccg atgtacgcga
     5401 acagccgcgt cgacgtgagc tcgaggacga ggaccgcgaa cgcgacaacc gcgatcgtcg
     5461 ttgtcggacg atgattgctc gtgcgcagtg ccgcgcagcg tatcatcgag cctcgcgcgt
     5521 ctcacgggct caccgagcga cggcagaccc ggcaactcga gtcgccatcg cggaacggcg
     5581 ctgtgtggaa tcgggtagat cgtgcggcga atcgcgcgga tcggcgggca cgtgagctgc
     5641 aacgccttgc cgcgatgcag tcgtccctgg ccatcgcgct tgcctgcgtg acgcttaccg
     5701 catgtgcggg cgtcgtcgac gagaccggct ccggcgacga tggcgctggc ggcgaggaga
     5761 tgtcacgtcc ggtcgatatc gaaggtcccg acgtcctgcc acacgtgcag cgcttcgcgg
     5821 atctcgtgtg tggctccctc gatgcctgcc acatctcgac gtacgccggc catcatccgg
     5881 tcgcgtcgcg cgcgatcgac atcctcgtca gcgacgtcta cggccgcgtg ccctcagacg
     5941 acaacgcgct cggtgatgcg gtcgcggcgt tcgcactcgc gcatcaggat cccgccggca
     6001 tcatgtacgt gatctggcgg cagcgttaca acgatggcag cggctgggat ccgatggagg
     6061 atcgcgg
//
LOCUS       DATLBZ010000064        10449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2285008, whole genome shotgun sequence.
ACCESSION   DATLBZ010000064 DATLBZ010000000
VERSION     DATLBZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285008"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VMZ53_03115"
     CDS             <1..750
                     /locus_tag="VMZ53_03115"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27464.1"
                     /translation="SPKNPVIKPFAMQVMKARRKTDYWDTHANFYSLLSLTTYAKAVA
                     GNAPSVSVKLGDKELITGTLSGKTKMRLVTAPLPQNATLEITPKGEVAYNVDIRYRAK
                     PETIKAEEHGIKLTREYLDEAGKPKTAFQVGDVVQVVLHVEVPSDSTNLMVSDGLPAG
                     FEALNTKLATVGGATVQQSRIWWGDYREMHDDRVDFASEYIWDGTFEYRYSIRAIASG
                     KFVRPPATAELMYDPKTNAQTALDFLEIKAK"
     gene            747..3023
                     /locus_tag="VMZ53_03120"
     CDS             747..3023
                     /locus_tag="VMZ53_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HUS27465.1"
                     /translation="MIALRLPERRRFRWPLLAVAGAFNALVLAWFIAVHVCTFPSELL
                     AKADATSLRVVDANGILLRQEATSAGTRERWMPLDRISPYLVSATLASEDNDFFEHDG
                     VDWTALARATWLNLRGGGIEFGGSTITMQLVRLTAGTSRSARGKLEQLVLAARLERTM
                     GKRDILEQYLNRVYYGHGAWGAEQAAQVYFGKHAADLAVGEAAFLSVLPRGPTYYDPF
                     RFQSRVMARRSHILALMEEKGRINAFDRALAERVPLDLDKHKPGFRAPHFVEMVKDRL
                     PPEFAQGAQVQTTLDWALQRQVQVALETHLDHVGSRHVGQAALVVMRNSDGAILALVG
                     SRDYHDDASNGAFNGATARLRPGSTLKPFVYGAAFERGDTPATIALDLVLSEDTHETY
                     STDVKSHGFARYRESLAGSYNLSAVHTLQRVGVETVLKKLRTAGLVTLDRADEDYDWG
                     LAIGHAETRLIDLTSAFSFVGRGGKPIAPRLVETATAVDGHVWRELEQPRPRVFSEEV
                     SYLLWDILSDPDARKPMFGDRVPMELPFKVALKTGTTKAYTDLWAIGVTREYTVGVWA
                     GNFDGSPTYHVMSVDGATPLVRATYAAIAARFGAPTAPDRPDTIVSADICPVSGMRPG
                     PHCEHRKREVFIRGQVPTETCNWHQIVCGAPALVYPPEIRPWASYYGRPEAPSCGEQG
                     IASGPLRITSPANGARFLLEPYRPATQQRPLLASSIPGARFTIDGSPAEQWVPVPGTH
                     HVVVTHGDEFDEVTITYE"
     gene            3020..3805
                     /locus_tag="VMZ53_03125"
     CDS             3020..3805
                     /locus_tag="VMZ53_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS27466.1"
                     /translation="MSNIAVITGAGRGLGRAIAERLAKRSYRVLVSDIDGDAAAKTAA
                     ELGNGAWSERQDVRDPDSHRAIAKAATQRGDVKVWINNAGVLTVGPTWEMADADVRRM
                     VEINVLGVMYGCHAAVPVMADDGHIINIASISGLTPTPGLAVYGATKHAVVGYSLSLA
                     GELRQAGRRIHVSALAPDAIQGDMTNAVAHDDASALLFSAGKLLTLDAVADAAVELLD
                     HPKHVKVLPAYRAAAIHALRPFPRLGLPLLDAMAKIGRWRTKR"
     gene            3823..4188
                     /locus_tag="VMZ53_03130"
     CDS             3823..4188
                     /locus_tag="VMZ53_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27467.1"
                     /translation="MRATIVLLLLVGCGRPAGSGMGAEEPQDPMPSSTLEHQNPAADT
                     SKNPTLHTYGSRWHWPSFFHFGGPPDPFASVGGAAPHYYTGCGGCSSHDPSNALIVFA
                     LMPLVVRRRRVRAARRPSP"
     gene            complement(4209..4439)
                     /locus_tag="VMZ53_03135"
     CDS             complement(4209..4439)
                     /locus_tag="VMZ53_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27468.1"
                     /translation="MKPVLTDALASVGATLAASVAALEHAGPGSVYVPIGVAGTFGAS
                     AVYGYMQVGRCRTEYRKRPTWTLEDAPAIMYR"
     gene            complement(4644..5387)
                     /locus_tag="VMZ53_03140"
     CDS             complement(4644..5387)
                     /locus_tag="VMZ53_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27469.1"
                     /translation="MESQLALEWLGGATERRLRTTRPDFDDLPWDSYDADAIAPDEAL
                     EARQVWTNGVFTEYASAAAFSAMNLAFLQCGAPVDLCAAAADFAVDELVHVSLVSRLV
                     MTMGGATPYVADLERVAPSPRPDLSARMRAAELAIKISCVGEALSLPALSRSHRGAKH
                     TLSRAVLEKLRHDEGPHASIGHWFLAWAEPWLTPGDREQLGRVARDAIAVYEPIWRDA
                     DDPAYRTYMEDAVEHRILRRLAKSHIDAR"
     gene            complement(5387..5806)
                     /locus_tag="VMZ53_03145"
     CDS             complement(5387..5806)
                     /locus_tag="VMZ53_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27470.1"
                     /translation="MTLDKETRRPRLRTASMLFGVVAASSTAGAATHYALCDREGAPA
                     CGNIGQLGREAPPPVEVHAEPAPAPITNTTVTHNAPLISITISTKLVAASAPAKPAVD
                     PLGPDFVIDPIPAKPVVDRAGDFAPGNVMSKGGPRRH"
     gene            complement(5904..6518)
                     /locus_tag="VMZ53_03150"
     CDS             complement(5904..6518)
                     /locus_tag="VMZ53_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27471.1"
                     /translation="MTKRDSPQAKLSSAMKYVVLVAGAVGMLGFFEPFFVFELGDRKV
                     DASPRALLLGFDDPKLEPLRYEEAPDCVKNVLQLDGGQVLGGYVCGQADKHKSYVPYY
                     FASTIIFVLLSLWAIVRRRMSGFAGVLTLPASFLAIGGWLREMKLDRLIESSHITTGA
                     HLLGVSGLIALAATIVLLVWREPPLLPKKKPVAKPTLPEARIVR"
     gene            complement(6515..7075)
                     /locus_tag="VMZ53_03155"
     CDS             complement(6515..7075)
                     /locus_tag="VMZ53_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27472.1"
                     /translation="MLRWMKVIVLVAGGLGVLAFFLPYYTFEYSDTHELVPASGYHLA
                     VGFPNPAHDHWRYQVRTRCMPDDQYCHEDLEGAELHHIRVPLYFWTALVFLVVGIVSI
                     WRKRMSIPAGFATLVASLFGVGLWLNELNHDWMTPESTPAIGCTLLGIAALLSLAPSI
                     AVLYWREPDPIPLFKIRVPQARVVRR"
     gene            complement(7115..8617)
                     /locus_tag="VMZ53_03160"
     CDS             complement(7115..8617)
                     /locus_tag="VMZ53_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HUS27473.1"
                     /translation="MSAAIRLIRATRVFAIIFLSYLWQMSWARLWPKRFGTSARWKKI
                     NRRNARRMYKGFVRLRGVYIKLGQILSIMGTFLPREYVEELEGLQDDVPPASYRKIAT
                     TFYKSFKKQPTEAFVSFNKEPIAAASLGQVHEARNTKGDRLAVKILYPNVATIIKVDL
                     RVLSWALRIYKNFVPLQQIERVHEQLADMLTRETDLVNEAAMIDRMTKNFAGDSDVVF
                     PVVYPEWSSHDVMTMTFMDGVKITKKSALSKLDLDPYAVATKLTKMFYKQLFIDRFFH
                     ADPHPGNFFVQKGPDGQPRIVVLDLGSATELTQNLADGMFDILQGLMTRKDDLVVKGI
                     HEMGFVAPDGNHALLERTVRKYFEKLLNLNITDFGKIDASVAQKLADPDMKREELREL
                     MKSVAYPEGWFYVERAAVIMFGLSATLAPKLNGIQVGFPYIMQFIMERNARRLAEART
                     SSPSIPVLPVPAPAPAPAPAPAPAAPSAPTSESTKKAEPSADGSDEALLN"
     gene            8673..9578
                     /gene="hemF"
                     /locus_tag="VMZ53_03165"
     CDS             8673..9578
                     /gene="hemF"
                     /locus_tag="VMZ53_03165"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HUS27474.1"
                     /translation="MSDLFDRAAKFFQSLQTDICRELATADGAKEFGGDAWQRPGGGG
                     GVARVLEGGALFEKAGVNWSNVDGELPAELAENMPGTGRAFRASGVSLVLHPHSPMVP
                     TTHANFRCIEKGDIVWFGGGADLTPYYVFREDAIHFHRTLADVCDQHAPVADYGRFKT
                     WCDEYFFLPHRGETRGVGGIFFDYLGSKGEHPAEDIFAFVQDLGRAFTRAYIPIAERR
                     QTEPFGEMERAWQLRRRGRYVEFNLIYDRGTLFGLKTNGRIESILMSLPPLVRWDYDV
                     MPQPGSPEAELIAHLRPTDWLGRTK"
     gene            9756..10148
                     /locus_tag="VMZ53_03170"
     CDS             9756..10148
                     /locus_tag="VMZ53_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27475.1"
                     /translation="MSRTTYERLKSFCETNNISMSQFVETRVGDFLGQNEGTTTVDII
                     RPTLPRPTTPSGPSPSPAKPALPVNKSETRPAAATSTTMSSPAQQVPMTSAAARFAVD
                     PPPPVIKPKQEQPAPKPAEKKADQIFTF"
BASE COUNT         1831 a         3389 c         3470 g         1759 t
ORIGIN      
        1 tcgccgaaga atccggtgat caagccgttc gcgatgcaag tcatgaaggc gcgccgcaag
       61 accgactact gggatacgca cgcgaacttc tactcgctgc tctcgctgac gacgtacgcg
      121 aaagcggtcg cgggcaacgc accgtcggtg agcgtgaagc tcggcgacaa ggagctgatc
      181 accggcacgc tctcgggcaa gacgaagatg cggctcgtga ccgcgccact gccgcagaac
      241 gcgacgctcg agatcacgcc gaaaggcgag gtcgcctaca acgtcgacat tcgctaccgc
      301 gcgaagcccg agaccatcaa agcagaggaa cacggcatca agctgacgcg cgagtatctc
      361 gacgaagcag gcaagccgaa gacggcgttc caggtcggcg acgtggtgca agtggtgctg
      421 cacgtggagg tgccgagcga ttctacgaac ctgatggtga gcgatggctt gcccgcgggc
      481 ttcgaagcgc tcaacacgaa gctcgccacc gtcggcggcg ccacggtgca gcagtcgcgc
      541 atctggtggg gcgactaccg ggagatgcac gacgatcgcg tcgacttcgc gtcggaatac
      601 atctgggatg gcacgttcga gtatcgctac tcgatccgcg cgatcgcgtc gggcaagttc
      661 gtgcggccac ccgcgacggc ggagctcatg tatgacccga agacgaacgc gcagaccgcg
      721 ctcgacttct tggagatcaa ggcgaagtga tcgcgctgcg gttgccagag cggaggcggt
      781 ttcgttggcc gctgctcgcg gtcgcgggtg cgttcaacgc gctcgtgctc gcgtggttca
      841 tcgcggtgca cgtctgcacg tttccgtcgg agctgctcgc caaagcagac gcgacctcgc
      901 tacgcgtcgt cgacgcgaac ggcatcttgc tgcgccaaga agcgacgagc gcgggcactc
      961 gcgagcgctg gatgccgctc gatcgcatct cgccgtatct cgtatctgcg acgctcgcgt
     1021 ccgaggacaa cgacttcttc gagcacgatg gcgtcgattg gacggcgctc gcgcgcgcga
     1081 cgtggctgaa cctgcgcggc ggcgggatcg agttcggcgg ctcgacgatc acgatgcagc
     1141 tcgtccggct gacggcgggg acatcgcgca gcgcgagggg caagctcgag cagctcgtgc
     1201 tcgcggcgcg cctcgagcgc acgatgggca agcgcgacat cctcgagcag tatctcaatc
     1261 gcgtgtacta cggccacggc gcgtggggcg cggagcaagc ggcgcaggtc tacttcggta
     1321 agcacgcggc cgacctcgcc gtcggagaag cggcatttct ctcggtgctg ccgcgcggcc
     1381 cgacgtacta cgatccattc cgtttccaat cgcgcgtgat ggcgcgacgc agtcacatcc
     1441 tcgcgctgat ggaggagaag gggcgcatca acgcattcga tcgcgcgctc gccgaacgcg
     1501 tgccgctcga tctcgacaag cacaagccgg gctttcgcgc gccacacttc gtcgagatgg
     1561 tgaaggaccg cctgccgccc gaattcgcgc agggcgcgca ggtgcaaacg acgctcgatt
     1621 gggcgctgca gcggcaggtg caagtcgcgc tcgagaccca cctcgatcac gttggctcgc
     1681 gtcatgtcgg gcaggcggcg ctcgtcgtga tgcgcaactc cgatggagcc atcctcgcgc
     1741 tcgtcggctc gcgcgattac cacgacgatg cgagcaacgg tgcgttcaac ggcgccaccg
     1801 cgcggctgcg gccgggctcg acgctgaagc cattcgtgta cggcgcggcc ttcgagcgtg
     1861 gcgacacgcc ggcgacgatc gcgctcgacc tcgtgctttc ggaggacacg cacgagacgt
     1921 actcgaccga cgtgaagagc cacggcttcg cgcgctatcg cgaatcgctc gcgggctcgt
     1981 acaacctctc ggcggtccac acgctccagc gcgtcggcgt cgagaccgtg ctcaagaagc
     2041 tgcgcaccgc gggcctcgtg acgctcgacc gcgccgacga ggactacgac tggggtctcg
     2101 cgatcggtca cgccgagacg cggctgatcg atctgacgag tgcgttctcg ttcgtggggc
     2161 ggggcggcaa accgatcgca ccgcggctcg tggaaacggc caccgccgtc gatggccacg
     2221 tgtggcgcga gctagagcag ccgcggccgc gcgtgttctc cgaggaggtt tcctatctgc
     2281 tgtgggacat cctcagcgat cccgacgcgc gcaagccgat gttcggtgac cgggtgccga
     2341 tggagttgcc gttcaaggtc gcgctcaaga cgggcacgac gaaggcgtac acggatctgt
     2401 gggcgatcgg cgtgacgcgc gagtacaccg tcggtgtgtg ggccggcaac ttcgatggct
     2461 cgccgaccta tcacgtgatg tccgtcgacg gcgcgacgcc gctcgtgcgt gcgacctacg
     2521 ctgcgatcgc tgcgcgcttt ggtgcgccga ccgcgccgga ccgtccagac acgatcgtct
     2581 cggcagacat ctgtcccgtc tcgggcatgc gtcccggccc gcactgcgag caccgcaagc
     2641 gcgaagtctt catccgaggc caagtgccga cggagacgtg caactggcat cagatcgtgt
     2701 gcggcgcgcc agcgctcgtg tacccgccgg agatccggcc atgggcgagc tactacggtc
     2761 ggccagaggc gccgtcctgc ggcgaacagg gcatcgcgtc cgggccgctc cgcatcacgt
     2821 cgcccgccaa cggcgctcgg tttctactcg agccctatcg ccccgcgacg cagcagcgcc
     2881 cgctgctcgc ctcgtcgatt cccggtgcgc gcttcacgat cgatggctcg cctgcagaac
     2941 aatgggtgcc ggttcccggg acgcaccacg tcgtcgtaac gcatggcgac gaattcgacg
     3001 aagtgacgat cacctacgaa tgagcaacat cgccgtcatc acgggcgcag gacgcgggct
     3061 cggtcgcgcc atcgccgagc gtctcgccaa acgcagctac cgcgtgctcg tcagcgacat
     3121 cgacggcgat gcggccgcga agaccgcagc cgagctcggc aacggcgcgt ggtccgagcg
     3181 tcaagatgtc cgcgatccgg acagtcatcg cgcgatcgca aaggcggcca cgcagcgcgg
     3241 tgacgtcaag gtctggatca acaacgcggg tgtgctcacc gtcggcccca cctgggagat
     3301 ggccgacgcc gacgtccgac ggatggtcga gatcaatgtg ctcggcgtga tgtacggctg
     3361 tcacgccgcg gtccccgtca tggccgacga cggtcacatc atcaacatcg cgtcgatctc
     3421 gggcctcaca ccgacgcccg gcctcgccgt ctacggcgcg acgaagcacg cggtcgtcgg
     3481 ctactcgctc tcgctcgcag gcgagctgcg gcaagccggc cgccgcattc acgtcagcgc
     3541 gctcgcgccc gacgccatcc aaggcgacat gacaaatgcg gtcgcgcacg acgacgcctc
     3601 ggcgctgctg ttctccgccg gcaagctgct cacgctcgac gccgttgccg acgccgcggt
     3661 cgaattgctg gaccacccca agcacgtgaa agttctgccc gcatatcgcg cggcggcgat
     3721 tcacgcgctg cgtccattcc cgcgcctcgg cctcccactg ctcgacgcga tggcaaagat
     3781 cggtcgctgg cgcacgaagc gctgaccagc aacaatagag cggtgcgggc aacgatcgtg
     3841 ctcctgctac tcgtcggatg cgggcgaccc gcaggctcgg gcatgggcgc ggaggagccg
     3901 caggatccga tgccgtcgtc gacgctcgag catcagaatc ccgccgccga cacgtcgaag
     3961 aacccgacgc tgcatacgta cggctctcgc tggcactggc cgagcttctt ccacttcggc
     4021 gggccgccgg atccattcgc aagcgtcggc ggtgcagcgc cgcattacta cacgggctgc
     4081 ggcgggtgct cgtcccacga tccgtcaaac gcgttgatcg tgttcgcact catgccgctc
     4141 gtcgtgcgtc gtcgtcgcgt tcgtgccgct cgtcgtccgt cgccgtagcc gtcgccatcg
     4201 cctcatagct agcggtacat gatggcgggc gcgtcctcga gcgtccatgt tggtcgcttg
     4261 cgatactccg tgcggcaccg gccgacctgc atgtagccgt acacggccga cgcgccaaac
     4321 gtccccgcaa cgccaatcgg cacgtagacg ctgccaggcc ccgcgtgctc gagcgctgcc
     4381 acacttgcgg cgagcgtggc gccgacactc gcgagcgcgt cggtgagcac cggtttcatc
     4441 tcgcggttgc acgcggtcgg atgcggcgcc ggtcgcgggc cctcggtcgc gaaaaacgaa
     4501 cacccggacg tggcggcgag caccgatatc gacgcgagga cagtccacgg gatggcacgc
     4561 atccgcgcaa gacacgcgct cgccgagcag gttgcgcctc agcgcgcgtc gatatgcgat
     4621 ttcgcgaggc gcgcagcgat cgatcaacgc gcgtcgatat gcgatttcgc gaggcggcgc
     4681 aagatgcggt gctcgacggc atcctccatg tacgtgcgat atgccgggtc gtccgcgtcg
     4741 cgccagatcg gctcgtaaac cgcgatcgca tcgcgcgcga cacggcccag ctgctcgcgg
     4801 tcgcccggtg taagccacgg ctccgcccac gcgaggaacc agtggccgat cgatgcgtgc
     4861 ggtccctcgt cgtgccggag cttctcgagc acggcgcgcg acagcgtgtg cttcgctcca
     4921 cggtgcgagc gcgacagcgc cgggagactc agcgcttcgc cgacgcacga gattttgatc
     4981 gcgagctccg cggctcgcat gcgcgcgctg agatccggcc gcggcgacgg agcaacgcgc
     5041 tcgagatctg cgacgtacgg tgtcgcgccg cccatcgtca tcacgagccg cgacacgagc
     5101 gagacgtgaa ccagctcgtc gacggcgaag tcggcggcgg cggcgcacag atcgacgggc
     5161 gcgccacatt gcaggaacgc gaggttcatc gccgagaacg ctgccgcgct cgcgtattca
     5221 gtgaacacgc cgttcgtcca gacttggcgc gcttcgagtg cctcgtcggg tgcgatggcg
     5281 tcggcgtcat agctgtccca cggcagatcg tcgaagtcgg gccgcgtcgt gcgaagccgt
     5341 cgctccgtcg cgccgccgag ccattcgagt gcgagttgcg actccatcag tgcctcctcg
     5401 ggccgccctt gctcatcacg ttgccaggcg cgaagtcacc ggcgcgatcg accacaggct
     5461 ttgccggaat tggatcgatg acgaagtccg ggccgagcgg atcgacggcc ggcttcgccg
     5521 gagccgacgc cgcgacgagc ttcgtcgaga tcgtgatcga gatgagcggt gcgttgtgcg
     5581 tcaccgtcgt gttcgtgatc ggggctggcg caggctcggc gtgcacctcc accggcggtg
     5641 gcgcctcgcg gccgagctga ccgatgttgc cgcaggccgg tgcaccttcg cgatcgcaca
     5701 gcgcgtagtg cgtggcagcg cccgcggtgc tcgaagcggc gacgacgccg aacagcatcg
     5761 aggctgtgcg caggcgagga cgacgtgtct ctttatcgag agtcatcgac acgacctcag
     5821 cgcttcacgg acaaggccga acaacacact cgctgtcttc atcagtgcct cctgtccatc
     5881 aagacgaggc agcctgcgaa gtgttaacga acgatgcgcg cctcgggcag agtgggcttg
     5941 gcgactggct ttttcttcgg gagcagcggt ggctcccgcc agacgagcag cacgatcgtg
     6001 gccgcaaggg cgatcaggcc ggacacaccc aggaggtgcg cgccggtcgt gatgtgcgag
     6061 ctctcgatca gccggtcgag cttcatctcg cgcagccacc cgccgatcgc gagaaagctc
     6121 gcgggaagcg tgagcacgcc ggcgaagccg ctcatgcggc gccgaacgat cgcccagagc
     6181 gagagcagca cgaagatgat cgtcgacgcg aagtagtacg gcacgtagct cttgtgcttg
     6241 tcggcttgcc cgcagacgta accgccgagc acttggccgc cgtcgagctg cagcacgttc
     6301 ttgacgcaat cgggcgcttc ttcgtagcgc agtggctcga gcttcggatc gtcgaagcca
     6361 aggagcaacg cgcgtgggct cgcgtcgacc ttgcgatcgc cgagctcgaa gacgaagaac
     6421 ggctcgaaga agccgagcat gcccactgcg ccggcaacga gcacgacgta cttcatcgcg
     6481 ctcgacagct ttgcttgcgg cgagtcgcgc ttcgtcaacg gcgcaccacg cgcgcctgcg
     6541 ggacgcggat cttgaataga gggatcggat cgggctcgcg ccaatacagc acggcgatcg
     6601 acggagcgag cgacagcagc gcggcgatgc cgagcagcgt gcagccgatt gcgggcgtgg
     6661 attcgggcgt catccaatcg tggttgagct cgttcagcca caggccgacc ccgaagaggc
     6721 tcgcgacgag tgttgcgaag cctgcgggaa tgctcatccg cttgcgccag atcgagacga
     6781 taccgacgac gaggaagacg agcgccgtcc agaagtagag cggaacgcga atgtggtgca
     6841 gctccgcgcc ttctagatct tcatggcaat actgatcgtc cggcatgcag cgcgtgcgga
     6901 cttggtagcg ccagtgatcg tgggcgggat tcgggaaccc gaccgcgagg tgatagccgc
     6961 tcgcgggcac gagctcgtgc gtgtcggagt actcgaacgt gtagtacggc aggaagaacg
     7021 cgagcacgcc gaggccgccg gccacgagca cgatcacctt catccagcgc aacacgcgcc
     7081 ggtcatacac cgaaacgaat cgcgcgagcc gcgattaatt cagcagcgct tcgtcggagc
     7141 cgtcggcgga aggctcggcc ttcttcgtcg attccgatgt cggggcgctt ggtgcagcgg
     7201 gtgcgggggc gggtgcaggt gcaggtgcag gtgcgggtac gggaagaacc ggaatcgagg
     7261 gcgacgacgt gcgggcctcg gcgaggcggc gcgcgttgcg ctccatgatg aactgcatga
     7321 tgtacgggaa gccgacctgg atgccgttga gctttggcgc gagcgtcgcg gacaggccga
     7381 acatgatgac cgcagcgcgc tcgacgtaga accagccctc cggatacgcg acggacttca
     7441 tgagctcgcg cagctcttcg cgcttcatgt ctggatcggc gagcttctgc gcgacgctcg
     7501 catcgatctt gccgaagtcg gtgatgttga ggttgaggag cttctcgaag tacttccgaa
     7561 ccgtgcgctc gagcagcgcg tggttgccgt cgggcgcgac gaagcccatc tcgtgaatgc
     7621 ctttgacgac gaggtcatcc ttgcgcgtca tcaggccctg aaggatgtcg aacatgccgt
     7681 ccgcgaggtt ctgcgtgagc tcggtcgcgc tgccgagatc gagcacgacg atgcgcggct
     7741 gaccgtcggg ccctttctgc acgaagaagt ttccagggtg cggatcggcg tggaagaagc
     7801 ggtcgatgaa gagctgcttg tagaacatct tcgtcagctt cgtcgcgacg gcgtatgggt
     7861 cgaggtcgag cttcgagagt gcagacttct tcgtgatctt cacgccgtcc atgaacgtca
     7921 tcgtcatcac gtcgtggctc gaccactcgg gatagacgac ggggaacacc acgtccgaat
     7981 cgcccgcgaa gttcttcgtc atgcgatcga tcatcgccgc ctcgttcacg aggtcggttt
     8041 cgcgcgtgag catatcggcg agctgctcgt gcacgcgctc gatctgctgg agcgggacaa
     8101 agttcttgta gatgcgcagt gcccagctaa ggacgcgtag gtcgaccttg atgatcgtcg
     8161 cgacgtttgg atagaggatc ttgacggcga ggcgatcgcc cttcgtgttg cgcgcttcgt
     8221 gaacctgccc gagcgacgca gctgcgatcg gttctttgtt gaaagagacg aaggcttcgg
     8281 tcggctgctt cttgaacgac ttgtaaaacg tggtcgcgat ctttcgatac gacgccggcg
     8341 gcacgtcgtc ctgcaagccc tcgagctcct cgacgtattc acgcggcaag aacgtgccca
     8401 tgatggagag gatctggccg agcttgatgt acacgccgcg gaggcggacg aagcccttgt
     8461 acatgcggcg cgcgttgcgc cggttgatct tcttccaccg agcgctcgtg ccgaaacgct
     8521 tcggccagag ccgcgcccac gacatctgcc acaggtaact gaggaagata atcgcgaaca
     8581 cgcgcgtcgc gcggatcaag cgaatcgcag ccgacatcga atcgagtctc tcgacgatga
     8641 tagcgcgcct ggctccgcgg tagcatcgcg gcgtgtcgga cttgttcgat cgcgcggcga
     8701 agttttttca gtcacttcag accgacatct gtcgcgagct cgctactgcg gacggggcca
     8761 aagagttcgg aggcgacgcc tggcagcgtc caggaggagg cggcggagtg gcacgcgtcc
     8821 tggagggcgg cgcgctgttc gaaaaagcgg gcgtgaactg gtcaaacgtc gacggcgagc
     8881 ttcctgcaga gctggccgaa aatatgccgg gaacgggacg cgcgttccgt gccagcggcg
     8941 tctcactggt tcttcacccg cactcaccga tggttccgac gacgcatgcg aatttccgct
     9001 gcatcgagaa aggcgacatc gtgtggttcg gcggaggcgc cgacctgacg ccgtactacg
     9061 tgttccgcga ggacgcgatt cacttccatc gcacgctcgc cgatgtgtgc gatcagcacg
     9121 cgccagtcgc ggattacggg cgcttcaaga cgtggtgtga cgaatatttc tttttgcccc
     9181 accgcggcga gacgcgcggc gtcggaggca tcttcttcga ttacctcggc agcaaaggcg
     9241 agcacccggc cgaggacatc ttcgcgttcg tccaagatct cggccgtgcg ttcacgcgcg
     9301 cctacattcc gatcgcggag cgccggcaga cggagccgtt cggcgagatg gaacgcgcgt
     9361 ggcagcttcg gcgccgcggc cgctacgtcg agttcaacct catctatgat cgcggcacgc
     9421 tgttcggtct caagaccaac ggccgcatcg agtcgatctt gatgtcgctg ccgccgctcg
     9481 tgcgttggga ctacgacgtc atgccgcaac cgggctcacc ggaggccgag ctcatcgcgc
     9541 accttcgtcc gaccgactgg ctcggccgca cgaagtagcg cgatccacgc gtcgcgcgcg
     9601 tgcgatcccg ttcgatcccg attcgatgtc aggggcatcg tgtagggtcc tttgcgttcg
     9661 cgggagtaca aggctagtag gttcgccttg acgaccccct acccgtagcg taaggattta
     9721 ttatggcgaa gaagcagact cgtcggagca tcagtgtgag ccgcacgacc tacgagcggt
     9781 tgaagtcgtt ctgcgagacc aacaacatct cgatgagcca gttcgtcgag acccgtgttg
     9841 gtgattttct gggtcagaac gaggggacga cgacggtcga cattattcgc cccacgcttc
     9901 cgcgcccgac cacgcccagt ggcccgagcc cgtcgcccgc caagccggcc ctgccggtga
     9961 acaagtcgga gactcgtccg gccgccgcga cctcgacgac gatgtcgtcg ccggcgcagc
    10021 aggttccgat gacgtcggca gccgcgcgct tcgcggtgga tccgcccccg ccggttatca
    10081 agccgaagca ggagcagcct gcgccgaagc cggccgagaa gaaggccgac cagatcttca
    10141 cgttctgagt cgaggatcgt cgtcgacgac gacgatcgtc cgatgcgcgc cgtctttcgc
    10201 cgcgcgtatc tctgtcgccc gaaaataaga acgggcccgg ctttggccag gcccgtttgc
    10261 tcaacgtacc agggccccac taggggagcc tgggtccggg aggattacga acgaacctcc
    10321 atcgagcggg tacagtgacg tttcatttgc gttcttcctc ccctggcctt agccagaagc
    10381 ccgacggcac ggcccgccag cagaattgca gcggctcccg tcatcgggcc ttcatgatgg
    10441 gcacttcgg
//
LOCUS       DATLBZ010000065         6958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311 k141_53085,
            whole genome shotgun sequence.
ACCESSION   DATLBZ010000065 DATLBZ010000000
VERSION     DATLBZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53085"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VMZ53_03175"
     CDS             <1..735
                     /locus_tag="VMZ53_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS27476.1"
                     /translation="RVWCAFSRSIARFDAKKNGALAKVEHGPELATSAMDETRHAGFV
                     LFHTANTHLSSFGGMSCGSCHIDGRADGVSWHIRGRELQTPVLAGRISGSGPFKWDGT
                     AKTLQRSLRETIDRLGGDGLSKKQLGQLATFVETLPAIRTPTRDVAAVARGKQLFQSP
                     AVGCTDCHDGAAFTDGARHRFGGDAFDTPGLAALAASAPYFHDGSAPTLDAVLRERGS
                     VHGMADASAELSERDIADLVAFLETL"
     gene            complement(732..1805)
                     /locus_tag="VMZ53_03180"
     CDS             complement(732..1805)
                     /locus_tag="VMZ53_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27477.1"
                     /translation="MMIKRSFAVLALACMVSSAAAAPKKKGGGKKLPPKQEEPAPPPE
                     PAPAPPPEPVSTEVKPWAEGVPQDQQDKARALYEEGNTLFGQQALTGAYEKYKEAIKL
                     WEHPLIRYNLAVTEIRLEKILDAAEDLEKALKWGDKPFKPELYQEAQNYQALIKGQVG
                     YVEVTCDQGGARLLFDGKPWFDCPGTKKLRVLAGVHAIVGEKKDFLTQSTKIVVTGDK
                     VEKANIKLLPLDTAVVLKYPYRRWIPWTMTAVGLVVGAGGAGTWFLGKQQMDKFHDAY
                     AVQCANGCEPGLTDPSHSSLAAQRDSAQLKGKIGIGMMGVGGAVAVTGIVLAIMNRPE
                     RVLPNVEVAPTSGGGVTSVGWSF"
     gene            complement(1802..5458)
                     /locus_tag="VMZ53_03185"
     CDS             complement(1802..5458)
                     /locus_tag="VMZ53_03185"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27478.1"
                     /translation="MAEPKGNDSGPAVRPSVLPPDDDDLDLALDRLGGDSPSPTPEAT
                     MPPQPALRPPAPRASQSIPRTTQPMAAVKPMVPTPANAPRPKSITAPRTSTPVTGAPS
                     VPLAAKPPTPPSAKPPATPPLRPPATPAAKPPLSPPRPTMTGAAPRPQTPVAGTAALN
                     GANGANGTNGAVADAVTAAPSGGNAIPEAIPHKMRSTPVPLVDDPPEAMPVAKRATGE
                     FAPRRPATDQEARGEIRTGGTPAPVERVSGEFPAAKPPSRQPKVSQATSLLPSDQQFP
                     SPEALSERSKPSADSKPKREPRKPIEIGDNTQIDRFGLIREIARGGMGQVFLARDTKL
                     GRKVAIKFLLHDDPNFAQRFLIEARATARVTHENIVAIYEVGEYQGLPYMVLEYLEGK
                     TLSQVLESKPGPKAFAEIMLSVARGLERAHEHGIVHRDLKPSNIFVTDRGQVKVLDFG
                     VARIFDAAGDVMEKAAAASGKDRNWESNTEGTGSRNTYVTFSGGGTLVGTLPYMSPEQ
                     WGADTVDHQSDIWACGIMFWRALTGVHPAGTMNADKLRARLGDLDTPLPSLGARDPSL
                     PHELVEIVDKSLRKRKSERYQNASEMIADLQAFLAPKADRFTEDVCPYRGLAAFGEQD
                     AKYFFGRSNEIRTALAQLDAWPLLAVIGPSGVGKSSFVHAGLVPAVRGTGGNWQVRVL
                     RPGRNPLQSLSATLDDTLDSGAVGADVLDGLSEAPGLYGERLRKAAHRKKQKVLIVVD
                     QLEELFTLSDDDEVRKLFLAALLAAADDPTSPVRVVLSMRADFLDRLAGHKHFLNELS
                     RGLFFLSAPDQDNLRETLVRPAELAGYTFGDPWIVEDMMQAATSKGALPLLQFAATRL
                     WDARDRQRKQLPISAYNQMGGVGGAFARHADEVAAAVPPQHQKLLRAIMTRLVTPEGT
                     RAVIDKQELLELSGDQGDVERIIDQLVRARLVLMHTDATQGTTVEIVHEVLITEWPTL
                     ARWLEEGQALRGFMAELRQATKQWVARKKSSDLVWRGATAQEALSTVKKHVVELSANE
                     KEFLDEARKGFARSRRRKVIVLSTIFITLVAILGAGAWFTIQLSQANAAAQEKAEEAE
                     KATKQVQAQLDEVKAAQAAQEKAQKEAAEKAAEALKANAAASQSQEDLAKANIELQKK
                     VEEALKANEVAQELARVAQENAKAAAAQKAAAEKATADAKAANARTQALLDKEKDRVK
                     QLEANQKKISTGGL"
     gene            complement(5536..6228)
                     /locus_tag="VMZ53_03190"
     CDS             complement(5536..6228)
                     /locus_tag="VMZ53_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS27479.1"
                     /translation="MEAAERARLVEAHLDLARKAASMIYPRVKDHVEYEELVGFANAG
                     LAEAAQRFDPARGASFKTFAWYRVQGSIIDGMRRGSNLPRRVWQQIVALRAASEYLEN
                     RAERDVGAAQQGTSPPEGADALAAMKSALSAIRTMYVTSLEAMREAGFDTADTTAPAG
                     DRLDTGRLSEKLRKALESLPERERALVTKHYWEGKNLLEAGEELGISKSWASRLHAQA
                     VEKLRTIVDAEL"
     gene            6377..>6958
                     /locus_tag="VMZ53_03195"
     CDS             6377..>6958
                     /locus_tag="VMZ53_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HUS27480.1"
                     /translation="MGTFTVLGEAELQHLANAFGLGTTLRSFRSIAAGTINSNFEVVT
                     ERGRFFIRVNEGKSEQDVAWEARLVGALAERGVVTPPPLPARDGRPYAPVAERDVPNA
                     TTRGDVLKWASAFAWRDGHHVHEPSEAQAEQFGAALAQLHVAGLELPVEWRRASIYDH
                     AHLLERFARIEATGDPQLAHAIDILRDELAIAGN"
BASE COUNT         1054 a         2371 c         2262 g         1271 t
ORIGIN      
        1 cgcgtgtggt gcgcgttctc gcgatcgatc gcgcgcttcg acgcgaagaa gaacggtgcg
       61 ctcgcgaagg tcgagcatgg gcccgagctc gcgacatccg cgatggacga gacgcggcac
      121 gcaggcttcg tgctgtttca caccgcgaac acgcatctgt cgtcgttcgg cggcatgtcg
      181 tgcggcagct gccacatcga cggtcgcgcc gatggcgtgt cgtggcacat ccgcggtcgc
      241 gagctgcaga cccccgtgct cgccggtcga atcagcggga gcggtccgtt caagtgggac
      301 ggcaccgcga agacgctgca gcggagcttg cgcgagacga tcgaccggct cggcggcgac
      361 ggactgtcga agaagcaact cggccagctc gcgacgttcg tcgagacgtt gcccgcgatt
      421 cggacgccga cgcgagacgt cgccgccgtc gcgcgcggta agcagctgtt tcaatcaccg
      481 gccgtcggct gcacggactg tcatgacggc gcagcgttca ccgacggtgc acgccatcgc
      541 ttcggcggcg acgcgttcga tacgcccggc ctcgcagcac tcgctgcgag cgcgccgtac
      601 tttcacgatg gcagcgcacc gacgctcgac gccgtgctcc gcgaacgagg cagcgtgcac
      661 ggaatggccg acgccagcgc cgagctctcc gagcgagaca tcgccgacct cgtcgcgttt
      721 ctcgagacgc tttagaacga ccagccgacc gacgtgacgc cgccaccact cgtcggcgcg
      781 acctcgacgt tcggcagcac gcgctcgggg cgattcatga tcgcgaggac gatgccggtc
      841 accgcgaccg caccgccgac gcccatcatg ccgatgccga tcttgccctt gagctgagcg
      901 ctatcccgct gcgctgcgag cgacgaatgc gacggatcgg tcagacccgg ctcgcagccg
      961 ttcgcgcact gcaccgcgta cgcgtcgtgg aacttgtcca tttgctgctt gccgagaaac
     1021 cacgtgcctg cgccgcccgc gccgacaacg aggccaacgg ccgtcatcgt ccacgggatc
     1081 cagcggcggt agggatactt cagcaccacc gccgtatcga gcggcagcag cttgatgttc
     1141 gccttctcga ccttgtcacc ggtgacgacg atcttcgtcg actgcgtcag gaagtctttc
     1201 ttctcaccga cgatcgcgtg aacaccggcg agcacgcgga gcttcttcgt gccgggacag
     1261 tcgaaccacg gcttaccgtc gaagagcagg cgtgcgccgc cttggtcgca cgtcacttcg
     1321 acgtagccga cctggccttt gatgagcgcc tgatagtttt gcgcttcttg gtagagctcg
     1381 ggcttgaacg gcttgtcacc ccacttgagg gccttctcga gatcctcggc agcatcgagg
     1441 atcttctcga gccggatctc ggtgaccgcc aagttgtagc gaatcagcgg gtgctcccag
     1501 agcttgatcg cttctttgta cttctcgtac gcgccggtca gcgcctgctg gccgaacagc
     1561 gtgttgcctt cttcgtagag cgcgcgcgcc ttgtcctgct gatcctgcgg cacaccttcg
     1621 gcccacggct tcacttcggt gctgaccggc tccggcggcg gagcaggggc gggctcgggc
     1681 ggaggtgccg gctcctcttg cttcggcggt agcttcttgc cgccgccctt cttcttcggc
     1741 gctgccgctg ccgacgagac catgcacgcg agcgcgagga cggcgaacga acgtttgatc
     1801 atcacagtcc cccggtcgag atcttcttct gattcgcttc gagctgcttc acgcggtcct
     1861 tctctttgtc gagcagcgcc tgcgtgcgcg cgttcgccgc cttggcatcg gcggtggcct
     1921 tctcagcggc agccttctgc gcagcggcgg cctttgcgtt ctcctgcgcg acgcgcgcga
     1981 gctcttgcgc gacctcgttc gcctttagcg cttcttcgac tttcttctgc agctcgatgt
     2041 tcgcctttgc gaggtcttct tgcgactgcg acgcggccgc gttcgccttc agcgcttcag
     2101 ccgctttctc cgccgcttcc ttctgcgcct tttcctgcgc agcttgcgcg gcctttacct
     2161 cgtcgagctg cgcctgcacc tgcttcgtcg ccttctcggc ttcttcggcc ttctcttgtg
     2221 cggcggcgtt agcctgcgag agctggatcg tgaaccacgc gcccgcgccg aggatcgcga
     2281 cgagcgtgat gaagatcgtc gagagcacga tcaccttgcg gcgacggctg cgcgcgaaac
     2341 ccttgcgagc ttcgtcgagg aattccttct cgttcgccga gagctcgacg acgtgcttct
     2401 tcaccgtcga tagcgcttct tgcgccgtcg cgccacgcca cacgaggtcg ctcgacttct
     2461 tgcgagcgac ccactgcttc gtcgcttggc ggagctcggc catgaagccg cgcagcgcct
     2521 gaccttcctc gagccagcgc gcgagcgtcg gccactcggt gatgagcacc tcgtgcacga
     2581 tctcgacggt cgtgccctgc gtcgcgtcgg tgtgcatgag cacgaggcgc gcgcgaacga
     2641 gctgatcgat gatgcgctcg acgtcgcctt gatcgcccga cagctcgagc agctcctgct
     2701 tgtcgatcac cgcgcgcgtg ccttccggcg tcacgagccg cgtcatgatc gcgcggagca
     2761 gcttctgatg ctgcggtggc acggcggcgg caacttcatc ggcgtgacgc gcgaacgcac
     2821 caccgacgcc acccatctgg ttgtacgcag aaatcggcag ctgcttgcgc tggcgatcgc
     2881 gtgcgtccca caagcgcgtc gcggcgaact gcagaagcgg tagcgcgcct ttgctcgtcg
     2941 cggcctgcat catgtcctca acgatccacg gatcgccgaa cgtgtagcca gcgagctcgg
     3001 cgggacgaac gagcgtctcg cgcaggttgt cttggtccgg cgccgagagg aagaacaagc
     3061 cgcgcgacag ctcgttcagg aagtgcttgt ggccggcaag acgatcgagg aagtcggcgc
     3121 gcatcgacag gacaacgcgc acgggcgacg tcggatcgtc cgccgccgcg agcagcgccg
     3181 cgaggaacag cttgcgcact tcgtcgtcat ccgagagcgt gaacagctct tcgagctggt
     3241 cgacgacgat cagcaccttc tgctttttgc gatgcgccgc tttgcgcagt cgctcgccgt
     3301 agaggcccgg cgcctcggag agaccgtcga ggacgtcagc acccacagcg ccgctatcga
     3361 gcgtgtcgtc gagcgtggcc gagaggctct gcagcgggtt gcgtcccggt cgcaggacgc
     3421 gcacttgcca attgccgccg gtgccgcgca ccgcgggaac gaggccagcg tgcacgaacg
     3481 acgacttgcc gacgcccgac ggaccgatga ctgcgagcag cggccacgcg tcgagctgcg
     3541 cgagcgcggt gcgaatctcg ttagagcgac cgaagaagta cttcgcgtct tgctcgccga
     3601 atgcagcgag tccgcgatac ggacagacat cctcggtgaa gcgatcggcc ttcggcgcga
     3661 ggaacgcctg caggtcggcg atcatctcgc tcgcgttctg atagcgctcg ctcttccgct
     3721 tgcggagcga cttgtcgacg atctcgacga gctcgtgcgg caagctcgga tcgcgtgcac
     3781 caaggctcgg cagcggcgta tcgagatcgc cgagtcgcgc gcggagcttg tctgcgttca
     3841 tcgtgccggc gggatgcacg cccgtcagcg cgcgccagaa catgatgccg cacgcccaga
     3901 tgtcgctctg atgatcgacg gtgtctgcgc cccactgctc ggggctcatg tacggcagcg
     3961 ttccgacgag agtgccgcca ccggagaacg tgacgtacgt gttgcggctg ccggtcccct
     4021 cggtgttgct ctcccagttg cgatcttttc cggaggcagc ggccgccttc tccatcacgt
     4081 cgccagccgc gtcgaagatg cgcgccacgc cgaagtcgag aaccttcacc tgaccgcggt
     4141 cggtcacgaa gatgttcgac ggcttgagat cgcggtgcac gatgccgtgc tcgtgtgcgc
     4201 gctcgaggcc gcgcgcgacc gacagcatga tctcggcgaa tgccttcggg cccggcttgc
     4261 tctcgaggac ctgcgagagc gtcttgccct cgagatactc gagcaccatg tacggcaggc
     4321 cctggtactc gccgacttcg tagatcgcga cgatgttctc gtgcgtgacg cgcgccgttg
     4381 cacgcgcttc gatgaggaag cgctgcgcga agttcgggtc gtcgtggagc aggaacttga
     4441 tcgcgacttt gcggccgagc ttcgtgtcgc gagcgaggaa cacttgcccc atgccgccgc
     4501 gcgcgatctc gcggatgaga ccgaagcgat cgatctgcgt gttgtcgcca atctcgatcg
     4561 gcttgcgcgg ctcgcgcttg ggcttcgagt cagcgcttgg cttgctgcgc tccgacagcg
     4621 cctccggcga cgggaactgc tgatcgctcg gcaacagcga cgtcgcttgg ctgaccttcg
     4681 gttgacggct cggcggcttc gcggcgggaa actcacccga cacgcgttcg acaggcgcag
     4741 gcgtaccacc ggtgcgaatc tcgccgcgcg cttcttgatc ggtggccggg cgacgcggcg
     4801 caaactcgcc ggtcgcacgc ttcgccacgg gcatcgcctc cggcggatcg tcgacgagcg
     4861 gaaccggcgt cgatcgcatc ttgtgcggaa tcgcttccgg aatcgcattg ccgccgctcg
     4921 gcgcagcggt caccgcgtct gcaacggcgc cgttcgtacc gttcgcaccg ttcgcgccgt
     4981 tcagtgccgc cgttccagca acgggagtct gcggccgcgg tgcggcaccc gtcatcgtgg
     5041 ggcgtggcgg cgagagcgga ggcttcgcag caggagtcgc gggtggacgc agcggcggcg
     5101 tcgctggagg ctttgcactc ggcggcgtag gcggcttcgc ggcaagcggc accgacggcg
     5161 ctccggttac gggcgtcgac gtgcgtggtg ccgtgatcga tttcggtcgc ggcgcattcg
     5221 ccggcgtggg caccatcggc ttcaccgccg ccatcggttg cgtcgtgcgc ggaatcgatt
     5281 gcgacgcgcg cggtgccggc ggacgcaacg caggttgcgg aggcatcgtc gcttctggag
     5341 ttggcgacgg cgaatcgccg cccaatcgat ccagtgcgag atctaaatcg tcatcgtccg
     5401 gcgggagcac agagggcctc accgccgggc cggaatcatt acccttgggt tccgccacgc
     5461 ctgaccatca ttgtagatcg acgttcccta cgctcgcgat gtaggtacgt ccaccacacg
     5521 cacgaccagc gatcgtcaca gctctgcgtc gacgatcgta cgaagtttct cgacggcctg
     5581 cgcgtgcagc cggctcgccc acgacttcga tatgccgagc tcttcacccg cttctagaag
     5641 gtttttcccc tcccagtaat gcttcgtgac gagggctcgc tcgcgttcgg gcaacgattc
     5701 gagcgctttg cgcagcttct ccgaaagtcg cccggtatcg aggcggtcgc cggcgggcgc
     5761 ggtcgtatcg gcggtgtcga aaccggcttc acgcatcgcc tcgagcgacg tcacgtacat
     5821 cgtccggatc gcagacagcg ccgacttcat cgcggccagc gcatcggcgc cttccggcgg
     5881 cgaggtcccc tgttgcgcgg caccaacatc gcgctcggcg cggttctcca agtactcgct
     5941 cgccgctcgc agcgcgacga tctgttgcca cacgcgccgc ggcaaattcg atccgcggcg
     6001 catgccgtcg atgatcgagc cctgcacgcg ataccacgcg aacgtcttga acgatgcgcc
     6061 gcgcgccgga tcgaaacgct gcgccgcctc cgcgaggccc gcgttcgcaa agccgacgag
     6121 ctcttcatac tcgacgtgat ccttcacgcg cggatagatc atggacgcag ccttgcgcgc
     6181 gagatcgagg tgcgcttcga ccagacgcgc gcgctcggca gcctccacgg atcgaacgta
     6241 ccaccgcccg cgctcctcgc gcttactgcg gtgatcgcat tcgccggcgc tgcccacgtc
     6301 acgagtcaga ggcgacgagc tcaattgctc gctgcctgcg tcgaggacga tagccgtctt
     6361 ctgctagctt ccgagcgtgg gcacgttcac cgtgctcggc gaggccgagc tgcaacacct
     6421 cgccaatgcg ttcggcctcg gtacgacgct gcgctcgttc cggtcgatcg ccgccgggac
     6481 gatcaactcc aacttcgagg tcgtaaccga gcgcggccgc ttcttcattc gcgtcaacga
     6541 gggcaagtcc gagcaggatg tcgcgtggga agcgcgcctc gtcggtgcgc tcgccgagcg
     6601 tggcgttgtc acgccgccgc cgctgcccgc gcgtgatggc cgcccgtacg cgcctgtcgc
     6661 cgagcgcgat gtgccgaacg caacgacgcg cggcgatgtg ctcaagtggg cgagtgcctt
     6721 tgcgtggcgc gatgggcacc acgttcacga accaagcgag gcgcaggccg agcagttcgg
     6781 cgcggcgctg gcgcagctgc atgtcgcggg gctcgagctg cccgtcgagt ggcgacgcgc
     6841 gagcatctac gatcacgcgc atctgctcga gcggttcgcg cgcatcgaag caacgggcga
     6901 tccgcagctc gcacacgcca tcgacattct gcgcgacgag ctcgcgatcg ccggcaac
//
LOCUS       DATLBZ010000066        27164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5270820, whole genome shotgun sequence.
ACCESSION   DATLBZ010000066 DATLBZ010000000
VERSION     DATLBZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 27164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27164
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5270820"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VMZ53_03200"
     CDS             <1..1377
                     /locus_tag="VMZ53_03200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS27481.1"
                     /translation="PAWDPAVKQAVLVTRVTNVVGARPAVRSAQLRVVDGPDRGLEIE
                     VPSVGVVIGSERSCDVVLTDAFVSRRHCSISPTAQGFTIVDLGSKNGTVIDGVAVQKV
                     VAPPGVAIRIGKTLVQLMPADEVVDIPPSTNDHFGALYGSSIAMRHVFAILERASKSN
                     APVLFLGESGTGKELMARGVHDASPRANGPFVVFDCGASTETLIESDLFGHTKGAFTG
                     AAGERVGAFAAAHGGTLFLDEIGDLPVSLQPKLLRMLEAGEVTPLGGRKSEKYDVRIV
                     AATHRDVFGEVARGGFRGDLYYRLAVVELHVPPLRQRTGDLKKLVEMFLVKAGAPKLA
                     SEVGGAALEKLERYHWPGNVRELRNVITRAVALAGPDDDFQSLPFVLRPTAAAPDEIN
                     FKADRPFHDAKDELVARFEREYLTDLVQRAGGNLSQAARIAGLERKFLYKLLERAGLR
                     TPEKDEAE"
     gene            1381..2733
                     /gene="argG"
                     /locus_tag="VMZ53_03205"
     CDS             1381..2733
                     /gene="argG"
                     /locus_tag="VMZ53_03205"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF003779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HUS27482.1"
                     /translation="MSRIFRQLPAKGTRLAIAYSGGLDTRCAVAWLAEQGMDVYAYTA
                     DLAQPDEKNPADIPPTALDHGARQARLIDCRDGLVREGFTAIQCGAFHLSVGGKRYFN
                     TTPLGRAVTTTAIVRAMVDDDVHVFGDGSTHKGNDIQRFYRYGILVDPTLRIYKPWLD
                     SAFVKAFGGRTEMAQYLAKIGKPYKTGVEKAYSTDANLLGATHEAKDLERLDVSMRIV
                     EPIMGVAHWKADVAVAAEEVTIGWEQGRPTSLNGQRFDSPFELFTEANKIGGRHGLGT
                     SDQIENRVIDAKSRGIYEAPGMALLHIAYERLLSAIHNENTLDLYATLGRRLGRLLYE
                     GKWFDPEACLLKDGLTRWVAPSVTGEVTLELRRGDDYSILATRAEYMSYGPDKLSMER
                     VTEPAFTPEDRIGALELQNLSVGDNRDLLLHHLSSLRQLAPTGGDPLGRLLGTPDPDE
                     "
     gene            2746..3723
                     /locus_tag="VMZ53_03210"
     CDS             2746..3723
                     /locus_tag="VMZ53_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27483.1"
                     /translation="MRWLALFALTGCQFVHGALTSARSDAAGEADADASIDAVDAPPD
                     TTGPNCYARWMSGNIAFSTPVLLSSLNSSGFDRDPFFEMNELTGYVSSARTGSTGGDI
                     WRATRSSLSGAFSTPALASEFNSVGNETKLSISENQRIAVVGSDRTGGEGGIDVWMST
                     RASATNPWPAMTQTGLSSVNSSIEEHDPTLSTDGLHLYLAPHDGSDQDLAMASRTSTS
                     QAFGSPARLTTLNSTSGDADPSPTPDELIIVFSSGRTLLGAANGNIWYSTRATKTAAW
                     AFATAVPSPVINTDAAEGDPHLSTDGCRLYFGRDSAANDNWEIYVATMQ"
     gene            complement(3724..6096)
                     /locus_tag="VMZ53_03215"
     CDS             complement(3724..6096)
                     /locus_tag="VMZ53_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27484.1"
                     /translation="MTGNDDTLPLERGPSVAERFAVGSVTAGRYRIDRFIASGGMGEV
                     YAARDLVLDTAVALKTLKPELEGSAQAIARLRREITLARAVTNAHVCRVYDVGEQEGR
                     VFLTMELLEGETLAERVKRGPLPVSDVERIAAQLVEGLSALHRVSVVHRDFKTSNVML
                     VADRAVITDFGLARSMETDSPRVTMSDEKGLLGTPAYMAPEQVEGRAATVASDIYALG
                     VVLFELLTGTLPFVEDNPIATATARLKRKAPAPSSVRDAIPARWDAIVVRCMEREPAA
                     RFSSVDDLLAYKPRSRRWFLAAGATAAASAALGAWRLAREEPRAPQRTSGVIALVPVL
                     GDGPWQLDAWRTAITVDIRDALATSGVPLLFMQQGGLGPALAGPASRVVEDPDPVAAA
                     LKIEGVATVITLALTRTATHVSLTMALAGAVHRAPRRFERPLDEVALLVEEVATASSV
                     ALGYGAIRPATDTRAIDAKLYEQYGAASSYAFRPRTDDDWTAIYEGRQERARDPLARL
                     AATSPALARAAAMVAEIEQLDGEQHEVASEVFEHLDLSDALTARALAIDPNQPLALAV
                     RGAVAMNRYNWKLAEQLTAYATALAPTHERVAYHRGMVLFLSGRFDEAIAHAEETQKR
                     SPKPRLGRAMLTWQYYYARRYDEVIRIAEPVWANFDRSHYAEAVWASFLALTYAEMAR
                     YDDALRIAEELRPVVDPYTLGGLVPVYVLAGRVDHARTIRDRIKDQVAMGIQVIMADA
                     LGETDVALDLLERVVASHNIHALFMRVERFSTRLRSEPRFQKLIATLGFP"
     gene            complement(6093..8477)
                     /locus_tag="VMZ53_03220"
     CDS             complement(6093..8477)
                     /locus_tag="VMZ53_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27485.1"
                     /translation="MGGHGGDETLRAGATDEHAAPERFAIGSIAAGRYRIVRYIAAGG
                     MGEVYAAHDLVLDTPVALKTLRPELEGSADAIARLRREIALARTVTDAHICRLHDVGE
                     HEGRVFLTMELLDGGTLAELVRGNGLPIAEVERIAAQLVAGLGALHRASIIHRDFKTA
                     NVIVVGDRAVITDFGLARSLDAKDARLTIESGLLGTPAYMAPEQVEARPATKASDIYS
                     LGVVLFELLTGKLPFDEDTAMATATARLTNDPPRPSSLRSGIPARWDAIVLRCLAREP
                     AQRFATVDDILGHRPVSRRWLLGAGAGAAAAAALGVWRMAARDGTTAAAVTRSPNEVI
                     AVLPVLGKGSWLTDAWRSAITFDLHDALATVGVPLLALLGHQMDLLAVGAAARAAQEQ
                     DPIAAARAFTNVATIVALELDRADVVTIRAAASGALEWNATFSRPANEIGLLVHEVAT
                     AIAVRLGYGLPHLPSDARALDAELYERYGSARASLYRPRTDFELIALREGKAEPPARA
                     IKTLARETPQLARAHVVAADVFAMAAEQSEDLPATQQQLDKAAELLNQAVAIDPANAF
                     AHAVLGHAAMLRWDWRRADEETRLASTLAPTHERVAYHRGNYLMLVGRFDEAIVAAER
                     DYERNPHPRLGRGILAWHYYYARRYDKVIGVLEPVIRNFDPTNALDRIIVSLLPPAYG
                     EMARFDDAVRVANELRPNLTPYDLAAFVSTYKLAGRNADAAALATSIREDVDLGAKAM
                     IADALGDTDETLRIVEQMVASHNIYAMFLRIERFSPTVRAHPRFQALLKTVGFP"
     gene            complement(8494..8961)
                     /locus_tag="VMZ53_03225"
     CDS             complement(8494..8961)
                     /locus_tag="VMZ53_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HUS27486.1"
                     /translation="MKKIIAALVFSSAACATTARTTTPDSPGTGMMPSLDMSSARGDN
                     ADAWFPALASEASLPSVANYQRELSTDRDRYDLAVRLCVAPNGSVASVELTQASGSER
                     LDRAVEHDIAAWQFAAFSAPSHIRVCKPLAFGYEPAAEASHLRIPLVRLSSRE"
     gene            complement(9084..10631)
                     /locus_tag="VMZ53_03230"
     CDS             complement(9084..10631)
                     /locus_tag="VMZ53_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS27487.1"
                     /translation="MVDDASTLMPAASRRAASADDVVMMTIAYHPDVSRVGERVVVTS
                     ELEVSRASPMFAPPGGGEARPLADRGLSRNPVVRIRPANGGVAFETVGNAMANLDGRP
                     LAGATARAWQTCDRAALDRGVVLEVADRIVLVLHLASASTERAARCGLVGDSDAIERV
                     RADISRVADLAVPVLVRGETGVGKELVAHALHQSSPRRDKAFLAINIAALPPTTAASE
                     LFGHGRGAFTGATHDHDGMFVRADGGTLFLDEVGETPLEIQPSLLRVLETSEVTPLGS
                     AKSRKVDVRLIAATDADLELQISESAFREALFHRLAGYQLIVPALRDRRDDIGRLLVH
                     FLRAELAATGDLDRLAKQVDAELWLPTSLVARLVRHAWPGNVRQLRNAARQLAIANRG
                     AAEAGSDAALDRLLATTAPPAASPVVAQVKRDPSAISDDELVAALQATGWSAVAAATR
                     LGVSRSSLYLLIDRCPKIRKAKDIAETELRRAFDDCAGELDAAAEILHVSRRALKLRW
                     TELGLVK"
     gene            complement(10647..12404)
                     /gene="coaE"
                     /locus_tag="VMZ53_03235"
     CDS             complement(10647..12404)
                     /gene="coaE"
                     /locus_tag="VMZ53_03235"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HUS27488.1"
                     /translation="MSAHVIGLTGGIASGKSAVARMLAERGAAVIDADQLARHIVEPG
                     QPALAELVARFGAAILTADGQLDRKRLGTIAFTDEDARRDLGRITHPRIAAASAQAIA
                     AWADAGANVVFYEAALLVENRAYAGLAGLVVVAAPPEVQEQRVMARDHLSLDEAKARI
                     AAQLPLAEKVKVATWVIENASDLAALEREVDRVVTEIEAKYGPIKAPSRGGAGTQAQA
                     RSREVALVTGFPAFTAKRMIAKLLASEPETKLYVLARDKFAPEADALLDQLGAGDRAE
                     VLVGDVCDMDLGLSSAEYRALSRELTWIHHLAGIYFMGVDDDTMRRVNVGGTRTVLEL
                     ARDAARLERLVHWSTAMVSGNRSGTVYEEDLDAGQKFHNGYERTKYEAEKLVRAAMRQ
                     LPITVLRPSIIVGDSRTGEIDRLDGPYYLMVLIATNASGLRLPILGRGDSPLHLVPID
                     YVVDAAWQVAHSPSAAGKTFHLADPNPMSARAVFEGVAEHAKTEKPRGSIPGPLARAV
                     LRTPGLSRLGRGPLSFLDLLDHTVHYDQTNTAQALAGKALVCPPLADYLAVLVQHVLD
                     VSRAPVAPTELDEIADPLD"
     gene            12443..12868
                     /locus_tag="VMZ53_03240"
     CDS             12443..12868
                     /locus_tag="VMZ53_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HUS27489.1"
                     /translation="MRSVVMMSLLLCGVANAGPRDWASSATCGKCHPAELASWQTTRH
                     AMTRDRFAEKPPAKCLGCHGTGEAPAGPAVVEVGCEACHGAGAAYATDDLMRNAFAAK
                     ALGLADLSTPKARTAVCMTCHSRMTKKLDLSAPVHPVAK"
     gene            12865..13440
                     /gene="yihA"
                     /locus_tag="VMZ53_03245"
     CDS             12865..13440
                     /gene="yihA"
                     /locus_tag="VMZ53_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HUS27490.1"
                     /translation="MKVKARFVISASKPSEFPAENLPEIAVVGRSNVGKSSLINSLVG
                     QDGLARTSRTPGRTRLVNWFEIDSKFHLVDLPGYGYAEVSRDMRESWRPLIESYLGER
                     KTLAGVLMLIDVRRGPEEEELDFVPWLEAKKIPIVVALTKADKLAKNKRMLEVSKAKK
                     ALGLKRDPFPVSAQTGDGIEPVFRALLQLLK"
     gene            13437..13889
                     /gene="rimI"
                     /locus_tag="VMZ53_03250"
     CDS             13437..13889
                     /gene="rimI"
                     /locus_tag="VMZ53_03250"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HUS27491.1"
                     /translation="MIDLDITPATEADLDAIDEIEQHSFKSPWTRATFEGELKREWAR
                     IDVVRTGSRIIGFCNYWLVTTELHILAIATHPDYRGKAIATRLLQHVLDTARAAGCSL
                     ATLEVRRSNKPAIALYERAGFKTVHVRARYYQDDGEDALVMLRGITAD"
     gene            complement(13876..14709)
                     /locus_tag="VMZ53_03255"
     CDS             complement(13876..14709)
                     /locus_tag="VMZ53_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS27492.1"
                     /translation="MRVALAMAAVAMLASVAHADDLDPRRKVVVLEYRASSSALPGIA
                     SVLVGEMTNQTSLQLLGPDQTRTIYGDGLDQAVVKCAGEAECVAKIGAKVGAKEVVLV
                     GVSEVGDVILTMQRIDVAKRTVTARLAELVTAEQTPTNEQISSYLQRLLPPGDFKRFG
                     VIEIRSSETGAVVSIGGEKRGVTPIEPLKLPAPAAYAIRVEKEGFVPFTTKVQLPPSS
                     ELTVDAELSRRGATAHWYTKWYVLAGIGVVSAGLVGGGIYFATREPATSVGVNGMVSP
                     P"
     gene            complement(14709..16331)
                     /locus_tag="VMZ53_03260"
     CDS             complement(14709..16331)
                     /locus_tag="VMZ53_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27493.1"
                     /translation="MREMRRVLVLSCVVAGCTGQGSLDLQLSLPTAPELRPTGMTTVE
                     VLATSPNIEPIANRSVLNGSFFSAGELPVADDVQLSVLLHDVSNRLVGLGEAPALVDI
                     VGDKATTVSIPVRRPFIYASSGTTLYTFDPTLDPRDGKFQGKLDGLTTPQVAVSVGGD
                     RLVVGGQSTLQVIDTATHKVTGGPIAMPGGGMIKDVAPVPGANIVAVAHTGGIAIVNL
                     ADGSVQNAEVGPVDRIAVGQAPDKRMVAYGLVGRVAPADSQLSACGGSSSIVAVFVDA
                     PAATAPKPLPMGASAIAAAPSQAAVFATIPCQGHVVKIEGDPTSEVATLTFTMMSMLP
                     NASTIAVLGDRVFAAGTRGSSPVCATASGNVTQCTTTTQVACPEPNATHLSYVTAGAR
                     LVVQSIPVGGGNPVELQLPEERETMISTDDPAEQHAQVLHPLSSVPLDLVALPGGQYV
                     SLVTKNSYYIESLSDGFTVLLPCLKVTTGNWYLIDMASSSVAQRVRPSCDLVYGMNAY
                     FRMWKCDNAPEGQRPTLGEFMPVGVGALFGAR"
     gene            16423..18273
                     /locus_tag="VMZ53_03265"
     CDS             16423..18273
                     /locus_tag="VMZ53_03265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27494.1"
                     /translation="MQTSVDKGQTGPLGRVDPSLPQVFGRYLLVQRLSRGGMGEIFLA
                     KHGLAGFEKLAVIKKVLPHLAADAQFISRFVDEAQVAIKLQHVNVAQVFEVGRVGDEY
                     FLALEYVEGRDLRRTLALLQQRAQRLPVDLALFIGRELANGLAYAHRRTTSDGSSLNL
                     VHCDISPPNVLVSFEGETKVIDFGIAKSAMRGTATDPKMGFGKFGYMAPEQLIRGGIV
                     DHRTDIYAAGVVLFELLTNKRLYEAGPEPDYRALAKKVAKGEHTMPSDVAKELAPYDD
                     LVATALRPKAEDRYQSAAELRDAIQQALVHVNPTISTDQLGSYMRDLFSIEMTAQREL
                     HERVAKAHLEDFQDELTKETGTTVSFAMANMPLQAPPDSPRPPRLPARVTGQQRPRPP
                     TLPTDILPQQPPQPPPIPTAAIPTQELSLADGTGAVPPEDLSLPSEKQRKSKAPLVIL
                     LVAAIAAVAVAGFVILGKSEKKPDRVAQQTPKKIVVEAISPEDQTPKPEEKPPEKKVE
                     QKVEQADKVETPTPPPLPKGEKTERKKHHSSTEKKVVAPPPAVKSEVTKEVVTAKFRQ
                     AKAAYDAYKQKNGGRFDAEWNDLAGYQQYHPNDLEGFVQRIESFRAKLRE"
     gene            18340..18678
                     /locus_tag="VMZ53_03270"
     CDS             18340..18678
                     /locus_tag="VMZ53_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HUS27495.1"
                     /translation="MKKVEAIIKPFKLDEVKERLAEIGVHGMTVTEVKGFGRTGGKKE
                     VYRGSAYVVDFVPKVKVEIIVPNDNVQQVVNVISEAARTGKIGDGKIFITPVDEVIRI
                     RTGETGEDAI"
     gene            18696..20108
                     /gene="glnA"
                     /locus_tag="VMZ53_03275"
     CDS             18696..20108
                     /gene="glnA"
                     /locus_tag="VMZ53_03275"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HUS27496.1"
                     /translation="MTPTDLKELVKKHNIKMIDAKFVDFLGQWKHLTYPVERLEEGIE
                     EGFGFDGSSIAGWRAINSSDMLMKPDPTTAVLDPFLKTPTLSILCDVIDPITHEPYSR
                     DPRSLAKRAVAYLKSTGIADTAYFGPEAEFFIFDSIQYSEDPNNSFFNIDSDEAGWNN
                     RRNEPQGNKGFKPPTKGGYCVAGPTDQLSDLRTQMVMTMQAVGIVIETHHHEVASAGQ
                     AEIDMKYDTLVKMADQILWYKHIVKNVARMNGKTATFMPKPLFGDNGSGMHTHQSLWK
                     SGKPLFAGDAYAGMSEMALFYIGGLLKHAAAICAFANPTTNSYRRLVPGYEAPVNLAY
                     SARNRSASIRIPMYSSSPKAKRLELRSPDPSCNPYLAFAAMMMAGLDGIENRIHPGEP
                     LDKDIYSLSPEELAEVPTVPGSLDAALDALRKDRAFLMKGDVFSADLLDAWIAYKSEA
                     EADHVRLRPVPAEFQLYYDC"
     gene            20253..21071
                     /gene="asd"
                     /locus_tag="VMZ53_03280"
     CDS             20253..21071
                     /gene="asd"
                     /locus_tag="VMZ53_03280"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HUS27497.1"
                     /translation="MPPVYSALVGATARRRLPVPLRSAAYRAFARVTGASLDEAELDL
                     RAYTSLGDLFARRLRPGARTVCEDYGAIIAPCDGVLAARGTAVDGALVQAKGRNYQLE
                     DLVVDAEYAEKLRGGDYATIYLSPKDYHRVHTPVDGTIVRYDYVPGALWPVNPVVATR
                     RDRLLVRNERVVIWIKAGRLGMVAIVMVAASGVGNMALSHAPESVVWRAAGEPRRIDV
                     GVEVQKGEELGAFRLGSTVVMVFEPGAVALDGEVGHVMRFGERMGTARGAASKS"
     gene            21068..22948
                     /locus_tag="VMZ53_03285"
     CDS             21068..22948
                     /locus_tag="VMZ53_03285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS27498.1"
                     /translation="MSKRDEDSQSDMEVGMTTAERRRRRRRGAGLRVPSDNVPRSRTP
                     TGAPPAPVPEDPALAMSIAYSFTNDASGPVPRAVNNESSAPVAPEAMATVIDPPSGPV
                     DADNFETQTREMSAVDLEALGLNSDGTSGLRMERMRTDPIAKTADLDVDVTLEEPVTA
                     QRPAVDVQKRVTAPMASAQPNRLQTVALSEDDLEELRPPTQPAGAKKSSRADVNKTIQ
                     LAAIDIEAMQKAAAQGEEPVSVSDIDIQDLAERAEQSGEIEVDVDEDKPSSSDNKPTK
                     QIAAPAAPATKPMRAQTEPPAPPRTQTGERKSGIIEAQHAPAAHPPAAPGKDHHPAPP
                     PPPPPAAAQHKPPPAPAKKPLPQAAVAAVVAKEPSKPTSRARPWFVDLFDEDYLRTLP
                     FLTPQATQAEAEFVINSMNLAAGAQVLDLGCGYGRHAMELAARGFHVVGLDLSTPLLV
                     RGGEEAHRRGLTINFVRGDMRELDFENQFDATYCLFSTFGYFDDETNKKTLQNVARAL
                     KPGGKVLVEILNRDYVIADLPTRVWWEGDGCVVLEEVELNYFSSRIQVNRSVVFDDGR
                     QLEQEISVRAYSLHEVGKLMHAAGFRVLEVSGGYQTRGRFFGNQSRHIIVLAERKETS
                     GT"
     gene            23047..24390
                     /locus_tag="VMZ53_03290"
     CDS             23047..24390
                     /locus_tag="VMZ53_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS27499.1"
                     /translation="MAATVSKVSPVVAPTARKNRVARVTAKRPKKEASDERERDPDSE
                     PELESEEEVVEKPARADGGTYLSMYFKDMALLDVLRPEEEFTAAREIEALEIMLWEAV
                     LAHPQAIERVLDAIQEVPDFVMPLEAKTLRRAAEGRDSKGFARAAARAARKLRSSDAD
                     HLFIDAALGAIDRMTLGIGVRGGDVATLGTRAKVGEWLRKVQSANRAANDARNDFVKA
                     NLRLVVSIARRFNHGRMALADLIQEGNLGLMKAVERYDYRRGFRFSTYASWWIRHAIS
                     RALADKGREVRLPVHMIDAQHRLTKARRQLTGKLGRQPTTEELAEATNMPPDKVEKMR
                     SWLLEQSVSIDKPVGDDEGRVLGEVLEDPDREVAGPTDDLEWVALTTEVQELLRELRP
                     IEADILRQRFGLGTEQELTLKEIGDKYNLSRERIRQLQEQALAKMRRALSRRDLL"
     gene            24436..24837
                     /locus_tag="VMZ53_03295"
     CDS             24436..24837
                     /locus_tag="VMZ53_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HUS27500.1"
                     /translation="MTRRLPLLPLLLLVLGLAACGSEDKKLKVTGIEPEKGDIEGGTY
                     VRIKGNRFIADGARNAKVYFGSRQGTVVRFASDSELIVEAPGGKPNEKVDVLIIFEPG
                     GELKIPNGFTFVEKNNEAPSVDQLDISKVKK"
     gene            complement(24858..26129)
                     /locus_tag="VMZ53_03300"
     CDS             complement(24858..26129)
                     /locus_tag="VMZ53_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS27501.1"
                     /translation="MTVCNRCGKENQDHYKFCLGCGAELIAAPKPGGGGDMGMMKTMM
                     AEPGAAGPGAPLGGGGPMGGGPMGGGPMGGGPMGGGPMRGPSGGMPNQGGPTGPGRPP
                     AGGMAGMPPSGPLGGGGPMGGGPMGGPPMAPPPMGPPPGVPAYAGFPPAPSAPVSASG
                     MRRCPSCGSDVPPTFRFCGTCGFRMDEQSAPNPMPGPPQPMGGGAPFAAPQPQRSRLS
                     MTLIRPDGSEGGTHDLRPGENKLGRSFGPVFENDGYLSPVHAMLDIRGVQAVVRDLDS
                     LNGVFVKMTQEEELQSGQIIRIGQELLRFEIIPTPEPTADGTELMGSPNPGYWGKLTV
                     IIGRDVTGAAFPLLGESVTLGRERGEINFPDDGYVSGLHARVTLRDGRVFLADLGSSN
                     GTFIKVNGERPVGHESFVLLGQQLFRLNFAG"
     gene            complement(26149..>27164)
                     /locus_tag="VMZ53_03305"
     CDS             complement(26149..>27164)
                     /locus_tag="VMZ53_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS27502.1"
                     /translation="LVCDACSALTPIGVPQCARCGQPVALDPRPRRTSKPPGANGGAV
                     ATASADAKAGVACTKCGTNVPPNFKFCQICGTRVVVAQDFNVETRVGPTSGAAAAAMP
                     QGSTGAQAKQGGRSTLFFGGPMQQARAKLTLIRGDGEDGVSFSLAGTDHLAGRGECPI
                     SFPDDPYLSPIHCNFRYANGALSVRDEGSLNGVYVRISGTVRIEPGTTVLVGEQVLSV
                     MQAKGFEDVPDTEGTYFSASMMRPATIEIVQQLRGGSSGWVFRVDTETVSIGRESNDI
                     NFPDDPFISGRHAQLQLTAGVLSVTDLGSRNGTFVRVTGERVLKHGDYVFLGQQLLRV
                     EIV"
BASE COUNT         4804 a         9019 c         8930 g         4411 t
ORIGIN      
        1 cctgcgtggg atcctgcggt aaagcaggcc gtgctcgtca cgcgcgtcac caacgtcgtc
       61 ggagcgcggc ctgccgtgcg gtccgcgcag ttgcgcgtgg tcgatggccc ggatcgcggg
      121 ctggagatcg aggtgccctc ggtcggtgtc gtcatcggca gcgagcgctc gtgcgatgtc
      181 gtgctgacgg atgcgttcgt gtcgcgccgg cattgctcga tctcgccgac cgcgcagggc
      241 ttcaccatcg tcgatctcgg cagcaagaac ggcacggtga tcgacggcgt cgcggtgcag
      301 aaggtcgtcg cgcctcccgg cgtcgcgatc cgcatcggca agacgctcgt gcagctgatg
      361 cccgccgacg aggtcgtcga catcccgccg tcgacgaacg accacttcgg cgcgctctac
      421 ggcagctcga tcgcgatgcg ccacgtgttc gccattctcg agcgcgccag caagtcgaac
      481 gcaccggtgc tcttcctcgg tgagagcggc accggcaagg agctgatggc acgcggtgtt
      541 cacgacgcat ctccgcgcgc gaatggcccg ttcgtcgtgt ttgattgcgg tgcgtcgacc
      601 gagacgctga tcgagagcga tctgttcggc cacacgaagg gcgcgttcac gggcgctgcc
      661 ggcgagcgcg tcggtgcatt tgcggcggcg catggcggca cgctgttcct cgacgagatc
      721 ggcgatctgc cggtctcgct gcagccaaag ctcttgcgca tgctcgaagc cggcgaggtc
      781 actccgctcg gtgggcgcaa atccgagaag tacgacgtgc gcatcgtcgc cgcaacgcac
      841 cgcgatgtgt tcggcgaggt cgcacgcggc ggctttcgcg gtgacttgta ctaccgcctc
      901 gcggtggtcg agctgcacgt tcctccgctg cgccaacgca caggcgatct gaagaagctc
      961 gtcgagatgt tcctcgtgaa agccggcgct ccgaagctcg cgagcgaagt cggcggcgcg
     1021 gctctcgaga agctcgagcg ctatcactgg ccgggcaacg tgcgcgagct gcgcaacgtg
     1081 atcacgcgcg cggtggcact cgccggtccc gacgacgatt ttcagtcgct gccgttcgtc
     1141 ttacgcccaa cggccgcagc gcccgacgag atcaacttca aggccgatcg cccgttccac
     1201 gacgcgaagg acgagctcgt cgcgagattc gagcgcgagt acctgacgga cctcgtgcag
     1261 cgcgccggcg gcaacctctc gcaggcagcg cgcatcgcgg gcctcgagcg caaattcctc
     1321 tacaagctcc tcgaacgtgc gggtctgcgc acccccgaaa aagacgaagc cgagtaatcc
     1381 atgtccagga tctttcgaca gctccccgcg aagggcacgc gcctcgcaat cgcatactcc
     1441 ggtggtctcg acacgcgctg cgccgtcgca tggcttgccg agcaaggcat ggacgtctac
     1501 gcgtacaccg ccgacctcgc gcagccggac gagaagaacc ccgctgacat cccgccgacc
     1561 gcgctcgacc acggtgctcg ccaggcgcgc ctgatcgatt gccgcgatgg cctcgtgcgc
     1621 gagggtttca ccgcgatcca atgcggcgcg tttcacctct ccgtcggtgg caagcgctac
     1681 ttcaacacca caccgctcgg gcgcgcggtc acgacgaccg cgatcgttcg cgcgatggtc
     1741 gacgacgacg tccacgtgtt cggcgatggc tcgacgcaca agggcaacga catccagcgc
     1801 ttctatcgct acggcatcct cgtcgatccg acgctgcgca tctacaagcc gtggctcgac
     1861 agtgcgttcg tgaaggcctt tggcggtcgc accgagatgg cgcagtacct cgcgaagatc
     1921 ggcaagccgt acaagacggg cgtcgaaaaa gcgtactcga ccgatgcaaa cctcctcggc
     1981 gccacgcacg aagccaaaga cctagagcgt ctcgatgtgt cgatgcggat cgtcgagccc
     2041 atcatgggcg tcgcgcattg gaaggcggac gtcgcggtcg cggcggaaga ggtgacgatc
     2101 ggttgggagc agggcaggcc gacgtcgctc aacggccagc gcttcgactc gccgttcgag
     2161 ctgttcaccg aggccaacaa gatcggtggc cggcacggtc tcggcacgag tgatcagatc
     2221 gagaatcgtg tgatcgacgc gaagagccgc ggcatctatg aagcgcccgg catggcgctg
     2281 ctccacatcg cgtacgagcg cctcctctct gcgattcaca acgagaacac gctcgatctc
     2341 tacgcgacgc tcggtcgccg gctcggccgc ctcctctacg aaggcaagtg gttcgacccc
     2401 gaggcatgcc tgctcaaaga tggcctcaca cgctgggtcg caccgagtgt cacgggcgag
     2461 gtcacactcg agctgcgccg cggggacgac tactccatcc tcgcgacacg tgcggagtac
     2521 atgtcgtacg gcccggacaa gctgtcgatg gagagggtca ccgagcccgc gttcacgccg
     2581 gaagaccgca tcggcgctct cgaattgcag aacctctcgg tcggcgacaa tcgcgatctg
     2641 cttctgcatc acctctcgtc gctgcgccag ctcgcgccga caggcggcga tccgctggga
     2701 cgattgctcg gcacgccaga tcctgacgag tgatacgatc gctgggtgcg ctggctcgct
     2761 ttgtttgcgt tgaccggctg ccaattcgtg cacggcgccc tgacgtccgc gagatccgat
     2821 gcggccggcg aggccgacgc cgatgcgagc atcgacgcag tcgatgcgcc tcccgatacg
     2881 acgggaccga actgttacgc gcgctggatg agcggaaaca tcgcgttcag cacgccggta
     2941 ctgctctcgt cgctcaactc gtcgggcttc gatcgcgatc cgttcttcga gatgaacgag
     3001 ctcaccggct acgtgagcag cgcgcgcacc ggcagcaccg gcggcgatat ctggcgagcg
     3061 acacgttcga gcttatcggg cgcgttctcg acgcccgcgc tcgccagcga gttcaacagt
     3121 gtgggcaacg agacgaagct ctcgatctcg gagaaccagc gcatcgccgt cgtcggtagc
     3181 gatcgcacag gcggcgaagg gggcatcgac gtctggatgt cgacccgcgc ctcggcgacc
     3241 aatccatggc cggcgatgac gcaaaccggc ctgtcgagcg tcaacagcag catcgaagag
     3301 cacgacccga cgctgagcac cgacggcctg cacctctacc tcgcgccgca cgacggctcc
     3361 gatcaggatc tggcgatggc gtcgcgcacg agcacgtcgc aggcattcgg cagcccagcg
     3421 aggctcacga cgctgaactc gacgtccggc gatgctgatc cgtcgccgac gcccgacgag
     3481 ctgatcatcg tgttctcgtc gggccgcacg ctgctcggcg ctgcgaacgg caacatctgg
     3541 tactccacgc gcgcgacgaa gaccgcagcc tgggcgttcg cgaccgcggt tccgtcgccg
     3601 gtgatcaaca cggacgcagc ggaaggcgat ccgcacctct cgacggatgg ctgccggctc
     3661 tacttcgggc gcgatagcgc cgcgaacgac aactgggaga tctacgtcgc gacgatgcag
     3721 tgatcacgga aagccgaggg tcgcgatgag cttctggaac cgcggctcgg agcgcaggcg
     3781 ggtcgagaat ctctcgactc gcatgaacag cgcgtggatg ttgtgagacg cgacgactcg
     3841 ctcgagtaga tccagggcaa catcggtctc gccgagcgca tccgccatga tcacctggat
     3901 gcccatcgcg acctgatctt tgatccgatc gcggatggtg cgcgcatggt cgacgcgtcc
     3961 cgcaaggaca tatacgggga ccaagccgcc gagcgtgtac ggatcgacga cggggcgcag
     4021 ctcctccgca atcctgagtg catcgtcgta gcgcgccatc tcggcgtatg tcagcgcgag
     4081 aaagctcgcc cagactgcct cggcgtaatg cgaccggtcg aaattcgccc acacgggctc
     4141 agcgatgcgg atcacctcgt cgtagcgtcg cgcgtagtag tactgccagg tgagcatcgc
     4201 gcggccgagg cgaggtttcg gcgatcgctt ttgggtctcc tccgcatggg cgatggcttc
     4261 gtcgaagcga ccggagagga acaagaccat gccgcggtgg taggcaacgc gctcgtgcgt
     4321 cggggcaagc gcggttgcgt acgccgtcag ctgctcggcg agcttccagt tgtagcgatt
     4381 catcgcgacg gcgccgcgta cggcaagcgc gagtggctga ttcggatcga tcgcgagcgc
     4441 gcgcgcggtg agcgcatcgg acagatcgag gtgctcgaag acttcgctcg ctacttcatg
     4501 ctgttcgcca tcgagttgct cgatctcggc gaccatcgcg gccgcacgtg cgagcgccgg
     4561 cgacgtcgcg gcgagccggg ccagtggatc gcgtgctcgt tcttgtcggc cctcgtagat
     4621 cgcggtccag tcgtcgtcgg tgcgaggccg gaacgcatag ctcgacgcgg caccgtactg
     4681 ctcgtacagc tttgcatcga ttgcgcgcgt gtcagtggcc gggcgaatgg cgccatagcc
     4741 gagcgcgacg gagcttgcag tggcgacttc ctcgacgagc agcgcgacct cgtcgagggg
     4801 ccgctcgaac cgacgaggtg cgcggtggac tgctccggcg agcgccatgg tcagtgatac
     4861 gtgcgtcgct gtcctcgtga gcgcgagcgt gatgacggtg gctacgcctt cgatcttcag
     4921 cgccgctgcg accggatcag gatcctccac cactctcgac gcgggacccg cgagcgcagg
     4981 gccgagcccg ccctgctgca tgaacagcag cggcacgccg gaggtggcga gagcatcgcg
     5041 aatgtcgacc gtgatcgcag tgcgccacgc atcgagctgc cacgggccgt ccccgaggac
     5101 aggcacgagc gcgatcacgc cactcgtgcg ctgcggcgcc cgtggctctt cacgcgcaag
     5161 ccgccatgcg ccgagcgcag ccgatgctgc ggccgtcgca cccgccgcga gaaaccaacg
     5221 ccgagagcga ggcttgtagg cgaggaggtc gtcgacgctc gagaaacgcg cagcaggttc
     5281 gcgctccatg cagcgcacga cgatcgcatc ccaccgcgct gggatcgcgt cgcgcacgga
     5341 ggagggggcc ggtgctttgc gcttgagccg cgcagtcgcc gtcgcgatgg gattgtcctc
     5401 gacaaacggc agcgtgccgg tgagcagctc gaacagcacg acaccgagcg cgtagatatc
     5461 gctcgcgacc gtcgccgcgc ggccttcgac ttgctctggc gccatatacg cgggtgtccc
     5521 gagcagccct ttctcgtcgc tcatcgtcac gcgcgggctg tctgtttcca tcgaccgcgc
     5581 gagcccgaaa tccgtgatca cagcgcggtc tgcgacgagc atcacgttcg acgtcttgaa
     5641 gtcgcgatgc accaccgaca cgcgatgcag cgcgctcaag ccttcgacga gctgcgccgc
     5701 gatccgctcg acgtcgctca ctgggagcgg gcctcgcttc acgcgctcgg cgagcgtctc
     5761 gccttcgagc agctccatcg tcaagaagac gcggccttct tgctcgccga catcataaac
     5821 gcggcagaca tgcgcgttcg tgaccgcgcg agcgagcgtg atttcgcggc gcagtcgggc
     5881 gatggcttgt gccgaacctt cgagctccgg cttcaacgtc ttcagcgcca ccgccgtatc
     5941 gaggacgaga tcgcgcgccg cgtagacctc gcccattccg ccgctcgcga tgaaccggtc
     6001 gatccgatag cggcccgccg tgacgctgcc gacggcgaat cgttccgcaa cgctgggccc
     6061 gcgctcgagc ggaagcgtgt cgtcgttgcc ggtcacggaa agccgactgt cttcagcagc
     6121 gcttggaacc gcggatgcgc tcgcacggtc ggcgagaacc gctcgatccg cagaaacatc
     6181 gcgtagatgt tatggctcgc aaccatctgt tcgacgatgc gcagcgtctc gtcggtatct
     6241 ccgagcgcgt cggcgatcat cgccttcgca ccgaggtcga cgtcttcgcg aatcgacgtt
     6301 gcgagcgcgg ctgcgtcggc attgcgaccg gcgagcttgt acgtggacac gaaggccgcg
     6361 aggtcgtacg gcgtgaggtt ggggcggagc tcgttcgcca cgcgaaccgc gtcgtcgaat
     6421 cgcgccattt cgccgtaggc cggcggcagt aggctaacga tgatccgatc gagagcgttc
     6481 gtcggatcga agttgcggat cacgggctcg aggacaccga tcaccttgtc gtagcgccgg
     6541 gcgtagtagt agtgccaggc gagaatccct ctgccgaggc gcggatgggg attgcgttcg
     6601 tagtcgcgct ccgcggctac gatggcttcg tcgaatctgc cgacgagcat caggtagttg
     6661 ccgcggtggt acgcgacgcg ttcgtgcgtg ggagcgagcg tgctcgcgag tcgcgtctcc
     6721 tcgtcggcgc gccgccaatc ccagcgaagc atcgcggcgt ggccgagtac ggcatgtgcg
     6781 aacgcattcg cggggtcgat agcgaccgcc tgattcaaga gctcggccgc cttgtcgagc
     6841 tgctgctgtg tggctgggag gtcctcgctt tgttccgcgg ccatcgcgaa cacatcggcg
     6901 gcgacgacgt gagcgcgcgc gagttgcggg gtctcacgtg cgagcgtctt gatcgcgcgt
     6961 gcgggcggtt cggctttgcc ttcgcgcagc gcgatgagct cgaaatccgt gcgcggccga
     7021 tagagactcg cgcgggcgct gccgtagcgt tcgtacagct cggcatcgag cgcacgagcg
     7081 tcgctgggca gatgcggcag accgtagcca agccgaaccg cgatcgcggt cgcgacttcg
     7141 tgaacgagga gcccaatctc gttggctgga cggctaaacg tggcgttcca ctcgagcgct
     7201 ccgctcgccg ccgcgcgaat ggtgacgacg tctgcgcgat cgagctcgag cgcgacgatg
     7261 gtcgccacgt tggtgaacgc gcgcgcggcc gcgatcggat cctgctcctg cgcggcgcga
     7321 gcggccgcac ctaccgccag gaggtccatc tgatggccca gtagcgctaa aagcggcaca
     7381 ccgacggtgg cgagcgcgtc gtggagatca aaggtgatcg cactacgcca cgcgtcggtg
     7441 agccacgaac ctttgccgag caccggcagc acggcgatta cttcgttggg ggaacgcgtc
     7501 accgcagcgg cggtcgtccc atcgcgcgcg gccatcctcc atacgccgag cgcggccgcc
     7561 gccgccgcac ccgcgcccgc tccgagaagc caccgccgcg agacgggacg atggccaagg
     7621 atgtcgtcga cggtcgcgaa ccgttgtgct ggctctcgcg cgaggcaacg cagcacgatc
     7681 gcgtcccagc gagcgggaat cccggagcgc agcgatgacg gccgcggcgg atcattcgtg
     7741 aggcgtgcgg tcgccgtcgc catggccgtg tcctcgtcga acggcagctt cccggtcagc
     7801 agctcgaaca ggacgacacc cagcgagtag atgtcgctcg ctttcgtggc gggacgcgcc
     7861 tcgacctgtt ccggcgccat atacgcgggc gtcccgagca aacccgattc gattgtcagt
     7921 cgtgcgtctt tggcatcgag cgaacgcgcg aggccgaagt cggtgatgac cgcccgatcg
     7981 ccgacgacga ttacgttggc cgtcttgaag tcgcgatgaa tgatcgatgc gcggtggagc
     8041 gctccgagac cggccactag ctgcgccgcg atgcgttcga cttcggcgat cggaagaccg
     8101 ttgccgcgaa cgagctcggc gagcgtccct ccgtcgagga gctccatcgt caggaatacg
     8161 cggccttcgt gctcgccgac atcgtgcaac cggcagatgt gcgcatcggt gacggtgcgc
     8221 gcgagcgcga tctctcgacg caggcgtgca atggcgtcgg ccgatccttc gagctccggc
     8281 cgcagcgtct tcagcgcgac gggcgtatcg agtacgagat cgtgcgcggc gtagacctct
     8341 cccattccac ccgcggcgat gtagcgcacg atgcggtagc ggccggctgc gatcgaacca
     8401 atcgcgaacc gctcgggcgc agcatgctca tccgttgccc ccgcacgcag ggtctcgtcc
     8461 ccaccgtgtc cacccatcgc gggccacgat atctcattcg cggctgctga ggcgcacgag
     8521 cggaatccgc aggtggctcg cctcggctgc gggctcgtaa ccaaacgcga gcggcttgca
     8581 gacgcgaatg tgcgacggcg cagaaaacgc ggcgaactgc cacgcagcga tgtcgtgctc
     8641 gaccgcgcga tcgagacgtt ccgagccgct ggcttgcgtc aactcgacgc ttgcgacgga
     8701 gccgttcggc gcgacgcaga ggcgcaccgc gagatcgtag cgatcgcgat ccgtgctgag
     8761 ctcgcgctgg tagttcgcga cgctcggaag cgaggcctcc gaggcgagcg cgggaaacca
     8821 cgcgtccgca ttgtctccac gagcgctgga catatcgagc gacggcatca tgccggtgcc
     8881 gggcgagtcc ggcgtggtcg tgcgagcagt ggtggcgcag gcggcagacg agaacacgag
     8941 agcggcgatg atcttcttca tggttgtgac ctccgacagg tccttagagc acctgcgatg
     9001 ccagctgctc cgcaagccat ctcgcgtact tggcgcgtcg gacgagccgc gtggacgtcc
     9061 gacgacgtct gcgtggacgt ccgctacttc acgaggccga gctcggtcca acgcagcttg
     9121 agcgcgcgcc gcgagacgtg aaggatctct gccgcggcat cgagctcgcc agcacagtcg
     9181 tcgaacgcac gccgcagctc ggtctccgcg atgtccttcg ccttgcggat cttcgggcag
     9241 cggtcgatca agagatagag gctcgagcgc gagacgccga gacgcgtagc cgccgcgacc
     9301 gcgctccacc ccgtggcttg cagggcagcg actagctcgt catcactgat cgccgatgga
     9361 tcgcgtttga cctgcgcgac gacaggcgac gcggccggcg gagcggttgt agcgagcaag
     9421 cgatcgagtg cagcgtcact tcccgcctcg gcagcgccgc gatttgcgat cgcgagctgg
     9481 cgtgccgcgt tgcggagttg gcgcacgttg ccgggccacg cgtgacgaac gagccgtgcg
     9541 acgaggctcg tgggcagcca cagctccgcg tcgacttgct tcgcgaggcg atcgagatcc
     9601 ccggtcgctg cgagctcggc ccgcagaaag tggacgagca accggccgat gtcgtcgcgc
     9661 cggtctcgca gcgcggggac gatcaactga taaccagcga gccgatgaaa cagtgcctca
     9721 cgaaatgccg actcgctgat ctgcagctcg aggtcagcat cggtggcggc gatcaagcgg
     9781 acgtccacct tgcgagactt cgcgctgccg agtggcgtga cctctgatgt ctcgagcacg
     9841 cgcagcagtg atggctggat ctcgagcggc gtctcgccga cttcgtcgag gaacagcgtg
     9901 ccgccatctg cgcgcacgaa catgccgtcg tgatcgtgcg tcgcacccgt gaatgcaccg
     9961 cggccatggc caaacagctc cgacgcggcg gtcgtcggcg ggagcgctgc gatgttgatc
    10021 gcgaggaatg ccttgtctcg ccgtgggctc gactgatgaa gcgcgtgcgc gacgagctcc
    10081 ttgccgacgc cggtttctcc acgcacgagc accggaaccg caaggtccgc gacacgcgag
    10141 atatcggcgc gcacacgctc gatcgcgtcg ctgtcgccga cgagcccgca gcgtgcggct
    10201 cgctccgtgg acgcgctcgc gagatgcagc acgagcacga tacgatcggc tacttccagc
    10261 acgacgccgc ggtcgagcgc tgcgcggtca cacgtctgcc acgcgcgtgc cgtcgcaccg
    10321 gcaagcggcc ggccgtcgag gttcgccatt gcgttgccga ccgtctcgaa tgcgacgccg
    10381 ccgttggcag gcctgatgcg cacgaccgga ttgcgcgaca gaccacggtc ggcgagtggt
    10441 cgcgcttcac cgccgccggg tggcgcgaac atcggcgagg cacgtgagac ctcgagctcg
    10501 ctcgtcacga cgacgcgctc gccgacgcgc gagacatcgg ggtggtacgc gatcgtcatc
    10561 atgacgacgt cgtctgcgga cgccgctcgc cgcgacgcgg ctggcatgag cgtgctcgcg
    10621 tcgtccacca ccggtttcat cgtacctcag tcgagagggt ccgcgatctc gtcgagctcg
    10681 gttggtgcga ccggcgcacg cgagacgtcc agcacgtgtt gcacgagcac cgccaggtag
    10741 tcggcgaggg gagggcacac gagcgctttc ccggcgagcg cttgcgcagt gttcgtctgg
    10801 tcgtagtgca cggtgtgatc gaggagatcg agaaacgaca gcgggccgcg gccgagccgc
    10861 gagagacccg gcgtgcgaag gaccgcgcgc gcgagcggac ctgggatgct gccgcgcggc
    10921 ttctcggtct tcgcgtgctc cgcgacaccc tcgaacactg cacgcgcgga catcggattt
    10981 ggatcggcga ggtgaaacgt cttgccggca gcgctgggcg aatgcgcgac ctgccacgcc
    11041 gcatcgacga cgtagtcgat cggcaccaga tgcagcggcg aatcgccgcg cccgaggatc
    11101 ggcaggcgca ggcccgacgc attcgtcgcg atcagcacca ttaaatagta cggaccgtcg
    11161 aggcggtcga tctcgccggt gcgcgagtcg ccgacgatga tcgacgggcg gagcacggtg
    11221 atcggcagct ggcgcatcgc cgcgcgcacg agcttctcgg cttcgtactt cgtgcgctcg
    11281 tagccgttgt ggaacttctg tccggcgtcg aggtcttctt cgtacacggt cccgctgcgg
    11341 ttgcccgaca ccatcgcggt cgaccaatgg acgaggcgct cgagacgcgc agcatcacgc
    11401 gcgagctcga ggacggtgcg tgttccgccg acgttcacgc gtcgcatcgt gtcgtcgtcg
    11461 acgcccatga agtagatgcc cgcgaggtgg tggatccacg tcagctcgcg cgaaagcgcg
    11521 cggtactccg cgctcgacag cccgaggtcc atgtcgcaga catcaccgac gagaacttcc
    11581 gcgcggtcgc ccgcgccgag ctgatcgagg agcgcatccg cctcgggcgc aaacttgtcg
    11641 cgggcgagca cgtagagctt cgtctcgggc tcggacgcga gcagcttcgc gatcatgcgc
    11701 ttcgcggtga acgccggaaa gccggtgacg agcgccacct cgcgcgagcg cgcttgggct
    11761 tgcgtgcctg caccgccgcg cgacggcgcc ttgatcggcc cgtacttcgc ttcgatctcc
    11821 gtgacgacgc gatcgacttc tcgctcgagc gcggcgaggt ccgaggcgtt ctcgatcacc
    11881 cacgtcgcga ccttcacctt ttcggcgagc ggcagctgcg cggcaatgcg cgccttcgcc
    11941 tcgtcgaggc tgaggtggtc gcgagccatc acgcgttgct cttgcacctc gggcggcgcg
    12001 gcgacgacga cgagccccgc gagacccgcg tacgcgcgat tctcgacgag caatgccgct
    12061 tcgtagaaga cgacgttcgc gccggcgtcg gcccacgctg cgatcgcctg cgcactggcg
    12121 gccgcgattc gcgggtgcgt gatccgaccg aggtcgcggc gcgcgtcttc gtcagtgaat
    12181 gcgatcgtgc ccaggcgttt gcgatcgagc tgcccgtcgg cggtgaggat cgcagcgccg
    12241 aatcgcgcga cgagctcggc gagtgcgggc tggccgggct cgacgatatg gcgcgcaagc
    12301 tggtcggcgt cgatgacggc cgcgccgcgc tcggcgagca tgcgggcgac ggcgctcttg
    12361 cccgacgcaa tgccgcccgt cagtccgatc acgtgcgccg acacggatgt cagttaccac
    12421 ggacgaggta cagttggcgc ccgtgcgctc ggttgtgatg atgtccctgc tgctttgcgg
    12481 cgttgcaaac gcgggccctc gcgattgggc gagtagcgcg acgtgcggta aatgccaccc
    12541 ggccgagctg gcctcgtggc agacaacgcg gcatgcgatg acccgtgacc gcttcgcaga
    12601 gaagccgcct gccaagtgct tgggctgtca cggcactggc gaagcgccag ctggccctgc
    12661 ggtcgtcgaa gtcggttgcg aggcatgtca tggtgcgggc gctgcgtatg caaccgacga
    12721 cctgatgcgc aacgcgtttg ccgcgaaggc gctcggccta gccgacctct cgacgccgaa
    12781 agcgcgcacc gccgtctgca tgacctgtca ctcgcgcatg acgaagaagc tcgatctcag
    12841 tgcacccgta caccccgtgg cgaaatgaaa gtcaaagcgc gcttcgtgat ctcggcaagc
    12901 aagccgagcg agttcccggc ggagaacctc ccggagatcg cggtcgttgg ccggtcgaac
    12961 gtcggcaagt cgagcctcat caactcgctc gtcggccagg atggtctcgc tcgcacgtcg
    13021 cgcacgcccg gccgcacgcg cctcgtgaac tggttcgaga tcgacagcaa gttccacctc
    13081 gtcgaccttc ccggctacgg ctacgcggaa gtcagccgcg acatgcgcga gtcatggcga
    13141 ccgttgatcg agtcgtacct cggtgagcgc aagacgctcg cgggtgtgct catgctcatc
    13201 gacgttcggc gcggccccga ggaggaggag ctcgacttcg ttccttggct cgaggcgaag
    13261 aagattccga tcgtcgtcgc gctgacgaag gcggacaagc tcgccaagaa caagcgcatg
    13321 ctcgaggtct ccaaagcgaa gaaggcgctt ggcctcaaga gagatccgtt tcccgtctcg
    13381 gcgcagaccg gcgacggcat cgaaccggtg tttcgcgcgc tgctccagct gctcaagtga
    13441 tcgacctcga catcacgccg gcaaccgaag ctgatctcga tgcgatcgac gagatcgagc
    13501 agcactcctt caagtcgccg tggacgcgcg cgacattcga aggcgagctg aagcgagagt
    13561 gggcgcgcat cgatgtcgtg cgtaccggct cgcgcatcat cgggttctgc aactactggc
    13621 tcgtgactac cgagcttcac atcctcgcga tcgcgacgca tcccgactat cgcggcaagg
    13681 cgatcgcgac acggctcctc cagcacgtgc tcgataccgc gcgcgcggcc ggctgctcgc
    13741 tcgcgacact cgaggttcgc cgcagcaaca agcctgcgat cgcgctctac gaacgcgcgg
    13801 gattcaagac ggtccacgtt cgcgcgcgct actaccagga cgacggcgaa gacgcgctcg
    13861 tgatgctgcg cggaatcacg gcggactgac catgccgttg acgccgacgc tcgtcgccgg
    13921 ctcgcgcgtt gcgaagtaga taccgccgcc gacgagaccc gcgctgacga cgccgatgcc
    13981 cgcgagcacg taccacttcg tgtaccaatg cgccgtcgcg ccgcgcctcg acagctccgc
    14041 atcgacggtg agctcgctgc tcggcggcag ctgcaccttc gtcgtgaacg gcacgaagcc
    14101 ctctttctcg acgcggatcg cgtacgccgc gggtgccggc agcttcagcg gctcgatcgg
    14161 cgtgacgccg cgcttctcgc cgccgatgct gacgaccgcg ccggtctcgc tcgagcgaat
    14221 ttcgatcacg ccgaatcgct tgaaatcacc cggcggcaac aggcgctgca ggtagctcga
    14281 gatctgctcg ttcgtcggcg tctgctcggc ggtgacgagc tcggcgagcc gcgccgtcac
    14341 cgtgcgcttc gcgacatcga tacgctgcat cgtcaaaatc acgtcgccga cctcgctcac
    14401 accgacgagc acgacctctt tcgcgccgac ctttgcgccg atcttcgcga cgcactcggc
    14461 ctcgccagcg cacttcacga cggcctgatc caacccgtcg ccgtagatcg tgcgcgtctg
    14521 atccggcccc agcagctgga gcgacgtctg gttcgtcatc tcaccgacga gcacgctcgc
    14581 gatgccgggc agtgccgacg agctcgcgcg gtactcgagc acgaccactt tgcggcgcgg
    14641 atcgaggtca tcggcatgcg cgacgctcgc gagcatcgcg accgcagcca tcgcaagcgc
    14701 aactctcatc aacgcgctcc gaacagcgcg ccgacaccga cgggcatgaa ctcaccgagc
    14761 gtgggccgct gaccttcggg cgcgttgtcg cacttccaca tgcggaagta cgcgttcatg
    14821 ccgtagacga gatcgcacga cgggcgcaca cgctgcgcga ccgacgagct cgccatgtcg
    14881 atcaggtacc agtttcccgt cgtgaccttg agacagggca acaggaccgt gaacccatcg
    14941 ctgaggctct cgatgtagta gctgttcttc gtcacgagcg agacgtactg accgccgggc
    15001 agtgcgacca gatcgagcgg cacgctcgag agaggatgca gcacctgcgc gtgttgctcg
    15061 gccggatcgt cggtcgagat catcgtctcg cgctcttcgg ggagctgcag ctcgacggga
    15121 tttccgccgc cgactggaat cgattgcacg acgaggcgcg cacccgccgt gacgtacgac
    15181 agatgcgttg cgttcggctc ggggcacgcg acctgcgtgg tggtcgtgca ctgcgtgacg
    15241 ttgccactcg cagtcgcgca gaccggcgag gatccgcgcg tgccggctgc gaacacccga
    15301 tcgccgagca ccgcgatcgt cgacgcgttc ggcagcatcg acatcatcgt gaacgtgagc
    15361 gtcgcgactt cgctcgtcgg gtcgccctcg atcttcacga cgtgtccctg gcagggaatc
    15421 gtggcgaaca ccgccgcctg cgacggagct gccgcgatcg ccgacgcacc catgggcagc
    15481 ggctttggcg cagtcgcggc cggcgcgtcg acgaacaccg cgacgatcga agacgatccg
    15541 ccgcacgcgc tcagctgcga gtccgccggc gcgactctgc cgacgagacc gtacgcgacc
    15601 atccgcttgt cgggcgcttg acccaccgcg atgcgatcga cagggccgac ctccgcgttc
    15661 tggacgctgc catcggcgag gttcacgatc gcgatgccgc cggtgtgcgc gaccgcgacg
    15721 atgttcgcgc ccggtaccgg agcgacgtct ttgatcatgc cgccgccggg catcgcgatc
    15781 ggaccgccgg tgactttgtg cgtcgccgta tcgatcacct gcagcgtcga ctgcccgccg
    15841 acgacgagtc gatcgccacc gaccgagacc gcgacctgcg gcgtcgtcag accgtcgagc
    15901 ttgccttgga atttcccatc gcgaggatcg agcgtcgggt cgaacgtgta gagcgtcgtg
    15961 ccgctcgatg cataaatgaa gggacgccgg accgggatcg acacggtcgt cgctttgtcg
    16021 ccgacgatgt cgaccagggc aggggcctcg ccgaggccga ccagccggtt cgacacgtca
    16081 tgcaacagga cactcagctg aacatcgtcc gcaaccggca gctcgccggc cgagaagaag
    16141 ctgccgttga gcacgctccg gttcgcaatt ggctcgatgt tcggcgacgt tgcgagcact
    16201 tcgaccgtcg tcattccggt cggccgcagc tcgggcgccg tcggcagcga cagctgcaaa
    16261 tccagcgagc cttgccctgt gcatcccgcg acgacgcagg agagcacgag cacgcggcgc
    16321 atctccctca tgggccagac gccaagctac caaataaccc tgcggatcac cacggcttct
    16381 atcctgttga agcgtacggc gtccgcgtta ggatggagcc acttgcagac gtccgtcgac
    16441 aagggccaaa ccggaccact cggtcgagtg gatccgagcc tgccgcaggt attcggccga
    16501 tacctgctgg ttcagcggct gtcgcgcggc ggcatgggcg aaatctttct cgcgaagcac
    16561 ggtctcgccg gcttcgagaa gctcgccgtc atcaaaaaag ttctgccgca cctcgcggcc
    16621 gacgcgcagt tcatctcgcg cttcgtcgac gaggcacagg tagcgatcaa gctgcagcac
    16681 gtcaatgtcg cgcaggtctt cgaggtcggt cgcgtcggcg acgaatactt cctcgcactc
    16741 gagtacgtcg aaggccgtga tcttcgccgc accctcgcgt tgctccagca gcgcgcgcag
    16801 cgtctgcccg tcgacctcgc gttgttcatc ggtcgcgagc tcgcgaacgg cctcgcatac
    16861 gcgcaccgcc gcaccacgtc ggacggctcg tcgctcaacc tcgttcactg cgatatctcg
    16921 ccgccgaacg tgctcgtctc cttcgagggc gagacgaagg tcatcgactt cggtatcgcg
    16981 aagagcgcga tgcgcggcac cgcaacggat ccgaagatgg gctttggcaa gttcggctac
    17041 atggcgccgg agcagctcat tcgcggcggc atcgtcgatc accgcaccga tatctacgcc
    17101 gcaggcgtcg tgctgttcga gctgctgacg aacaagcgcc tctacgaagc gggtcccgag
    17161 ccggactacc gcgctctcgc gaagaaggtc gccaaaggcg agcacacgat gccgagcgac
    17221 gtcgcgaagg agctcgcgcc gtacgacgat ctcgtcgcga ccgcgctgcg cccgaaagca
    17281 gaggatcggt accagagcgc tgccgagctg cgcgatgcga tccagcaggc gctcgtgcac
    17341 gtgaatccga cgatctcgac ggatcagctc ggctcgtaca tgcgcgacct gttctcgatc
    17401 gagatgaccg cgcagcgcga gctgcacgag cgcgtcgcga aagcgcacct cgaagatttc
    17461 caggacgagc tgacgaaaga gaccggcacg acggtgtcgt tcgcgatggc gaacatgccg
    17521 ctgcaagcgc cacccgactc gccgcgaccg ccgcggttgc ccgcgcgcgt gaccggtcaa
    17581 caacgcccgc gtccgccgac gctgccgacg gacatcctgc cgcagcagcc gccgcagcct
    17641 ccgccgatcc cgaccgccgc gatcccgacg caggagctct ctctcgccga cggtaccggc
    17701 gcagttccgc ccgaagatct gtcgctgcca tccgagaagc agcggaagag caaagcgccg
    17761 ctcgtcatcc tgctcgtcgc agcgatcgcc gcggtcgcgg tcgccgggtt cgtcatcctc
    17821 ggcaagagcg agaagaagcc ggaccgagtc gctcagcaga cgcccaagaa gatcgtcgtc
    17881 gaggccatct cgcccgaaga ccagacgccg aagccggaag agaagccgcc cgagaagaag
    17941 gtcgagcaga aggtcgaaca ggccgacaag gtcgaaacgc cgacgccgcc gccgcttccc
    18001 aaaggtgaga agaccgaaag gaagaagcac cactcgtcga ccgagaagaa ggtggtcgcg
    18061 ccgccgccgg cggtgaaatc cgaggtgacg aaagaggtcg ttaccgcgaa gtttcgccag
    18121 gcgaaggccg cgtacgacgc gtacaagcag aagaacggcg ggcgtttcga cgccgagtgg
    18181 aacgatctcg ccggctacca gcagtaccac ccgaacgacc tcgaaggctt tgtccagcgc
    18241 atcgagtcat ttcgcgcgaa gcttcgggag taaaaggcga tcaccttttt tccgggtcgg
    18301 aaacccacac gaaacaatgt gtgggctaac gatccggtca tgaagaaggt cgaggccatc
    18361 atcaagccgt tcaagctcga cgaggtcaaa gaacgcctcg ccgagatcgg tgtgcacggc
    18421 atgaccgtca ccgaagtgaa aggcttcggc cgcaccggcg gcaagaaaga ggtctatcgc
    18481 ggctcggcct acgtcgtcga cttcgtgccg aaggtgaagg tcgagatcat cgtgccgaac
    18541 gacaacgttc agcaggtggt caacgtgatc agcgaggccg cacgcacagg aaaaatcggc
    18601 gacggcaaga tcttcatcac gcccgtcgac gaagtcattc gcattcgcac cggtgaaacc
    18661 ggtgaagacg ccatctagag aggaagacaa cgaccatgac ccccacggat ctcaaagagc
    18721 tcgtcaagaa gcacaacatc aagatgatcg atgcgaagtt cgtcgacttc ctcggtcagt
    18781 ggaagcacct gacgtacccc gtcgagcgcc tcgaggaagg catcgaagag ggcttcggct
    18841 tcgacggctc gtcgatcgcc ggctggcgcg cgatcaactc gtccgacatg ctgatgaagc
    18901 ctgatccgac gacggccgtc ctcgatccat tcttgaagac gccgacgctg tcgatcctct
    18961 gcgacgtgat cgatccgatc acgcacgagc cgtactcgcg tgatccacgc agcctcgcga
    19021 agcgcgcggt cgcatatctc aagtcgacgg ggatcgcgga caccgcgtac ttcggtcccg
    19081 aagccgagtt cttcatcttc gattcgatcc agtactcgga ggatccgaac aactcgttct
    19141 tcaacatcga ttccgatgaa gccggctgga acaaccgccg caacgagccg cagggcaaca
    19201 agggcttcaa gccgccgacg aagggcggtt actgcgtcgc cggtccgacg gatcagctct
    19261 ccgatctgcg tacgcagatg gtgatgacga tgcaggccgt cggcatcgtg atcgagacgc
    19321 accatcacga ggtcgcctcg gcaggtcagg ccgagatcga catgaagtac gacacgctcg
    19381 tgaaaatggc ggaccagatc ctctggtaca agcacatcgt caagaacgtc gctcgtatga
    19441 acggcaagac ggcgacgttc atgccgaagc cgctcttcgg cgacaacggc tcgggcatgc
    19501 acacgcacca gtcgctgtgg aagagtggca agccgctctt cgccggcgat gcgtacgcgg
    19561 gcatgagcga gatggcgctc ttctacatcg gcggtctgct caagcacgcc gctgcgatct
    19621 gcgcgttcgc gaacccgacg acgaacagct atcgccgtct cgttccgggc tacgaagcgc
    19681 cggtcaatct ggcgtactcg gcgcgcaacc gctcggcgtc gatccgcatc ccgatgtact
    19741 cgagctcgcc gaaggccaag cgcctcgagc tgcggtcgcc cgatccgtcg tgcaacccgt
    19801 acctcgcgtt cgcggccatg atgatggccg gcctcgatgg catcgagaac cggatccacc
    19861 cgggcgagcc gctcgacaag gacatctact cgctgtcccc agaggagctt gctgaggtcc
    19921 cgacggtccc gggctcgctg gacgcggcgc tcgatgcact gcgtaaagac cgggcattcc
    19981 tcatgaaggg tgatgtgttc tcggccgatc tcctcgatgc gtggattgcc tacaagagcg
    20041 aggcagaggc ggatcacgtg aggctgcgcc cggttccggc cgagttccag ctgtactacg
    20101 actgctaagg ctgaccgaac gtgactttcc agtcacgtcg ttagcttgta actcggcgct
    20161 ctccctggca gaatctgtat atccgggggc gtgaagccac acgctctctt ggggaagcgc
    20221 cgagcgcccg agtggattat cggtcgataa aattgccgcc cgtctactcc gcgttggtcg
    20281 gagcgaccgc gcgtagacgg ctgccggttc cgctgcgaag tgcggcgtat cgcgcgtttg
    20341 cgcgtgtcac cggcgcgtcg ctcgacgaag cggagctcga tctccgcgcg tatacttcgc
    20401 tcggtgatct gttcgcgcgg cggctgcgtc cgggagcgcg cacggtctgt gaagactacg
    20461 gtgcgatcat cgcgccgtgt gatggcgtgc ttgcagcgcg tggcaccgcg gtggatggcg
    20521 cgctcgtcca agcaaaaggc cgcaactacc agctcgagga tctcgtcgtc gatgccgagt
    20581 acgccgagaa gcttcgcggc ggcgactacg cgacgatcta tctgtcgcca aaggactacc
    20641 accgcgttca cacgcccgtc gacggcacga tcgttcgcta cgactacgtg ccgggcgcgc
    20701 tgtggccggt gaatcccgtc gtcgcgacgc gccgcgatcg cctgctcgtt cgcaacgagc
    20761 gcgtcgtcat ctggatcaaa gctggccgcc tcggcatggt ggcgatcgtc atggtcgcgg
    20821 cgagcggcgt cggcaacatg gccctgagcc acgcaccgga atctgtcgtg tggcgcgcag
    20881 cgggtgagcc tcgccggatc gatgtcggcg tcgaagtgca aaagggcgag gagctcggcg
    20941 cattccgcct cggctcgacg gtcgtgatgg tattcgagcc tggcgcggtc gcgctcgacg
    21001 gtgaggtcgg gcacgtcatg aggttcggcg agcggatggg aacggcgcga ggagccgcca
    21061 gcaagtcatg agcaagcgcg acgaagactc gcagtccgac atggaagtcg gaatgacgac
    21121 ggcagaacgt cgtcgccggc ggcgtcgtgg cgcaggtctg cgcgtgccga gcgacaacgt
    21181 tccgcgttcg cgcaccccga cgggcgctcc gcctgcaccg gtccccgagg atccggcgct
    21241 cgcgatgtcg atcgcataca gcttcacgaa cgatgcatcg gggccggtgc ctcgcgcagt
    21301 gaacaacgag agctccgcgc cggtcgcgcc cgaggcgatg gcgacggtga tcgatccgcc
    21361 gagcggtccg gtcgatgccg acaacttcga gacgcagacg cgcgagatga gcgcggtcga
    21421 tctcgaagcg ctcggcctca actccgatgg cacgtcggga ctgcgcatgg aacgcatgcg
    21481 taccgatccg atcgcgaaga ccgccgacct cgatgtcgac gtgacgctcg aggagccggt
    21541 aaccgcgcag cgccccgccg tcgacgtgca gaagcgcgtc acggctccga tggcgtccgc
    21601 gcaaccaaat cgcttgcaga ccgtcgcgct ctccgaggac gatctcgaag agctccggcc
    21661 gccgacgcag cctgccggcg cgaagaagtc gtcgcgcgcc gacgtcaaca agacgatcca
    21721 gctcgctgcg atcgatatcg aggcgatgca gaaggccgcc gcgcaaggcg aggagcccgt
    21781 ctcggtctcg gacatcgaca ttcaggacct cgccgagcgc gccgagcaat cgggcgagat
    21841 cgaagtcgac gtcgacgagg acaagccgag ctcgagcgac aacaagccga cgaaacaaat
    21901 cgcggcaccc gcggcacccg caacgaagcc gatgcgcgcg cagaccgagc cgccggcgcc
    21961 gccgcgtaca cagaccggtg agcgcaagag cggaatcatc gaggcgcaac acgcgcccgc
    22021 tgcgcatccg ccggccgcac ccggcaagga tcatcatcca gcgccgccgc cgccgccacc
    22081 gccggccgcc gcgcagcaca agccgcctcc ggcgcccgcg aagaaaccgt tgccgcaggc
    22141 agctgtcgca gcggtggtgg cgaaagagcc gagcaagccc acgtcgcgtg cgcggccgtg
    22201 gttcgtcgat ctcttcgacg aagattatct gcgcacgctg ccgttcttga cgccgcaggc
    22261 gacgcaagcc gaagccgagt tcgtcatcaa cagcatgaac ctcgccgcgg gcgcacaggt
    22321 gctcgacctc ggttgcggct acggccgtca cgcgatggag ctcgcggcgc gcggcttcca
    22381 tgtcgtcggt ctcgatctct cgacgccgct gctcgtgcgc ggtggtgaag aagcgcatcg
    22441 ccgcggcctg acgatcaact tcgtgcgcgg cgacatgcgc gagctcgatt tcgagaatca
    22501 gttcgacgcg acgtactgtc tgttctcgac gtttggttac ttcgacgacg agacgaacaa
    22561 gaagaccctg caaaacgtgg cacgcgctct caagcccggc ggcaaagtgc tcgtcgagat
    22621 cttgaaccgc gactacgtga ttgccgacct gccgacgcga gtgtggtggg aaggtgacgg
    22681 atgtgtcgtt ctcgaggaag tcgagctcaa ctacttctcg tcgcgtattc aggtaaatcg
    22741 ttccgtcgtg ttcgacgacg gtcgccagct cgagcaagag atctcggtgc gggcatactc
    22801 actgcatgag gtcggtaagc tcatgcatgc ggcgggcttc cgagtgctcg aagtatcggg
    22861 cggttaccag acccgaggcc gattcttcgg caaccaatcg cgccatatca ttgtcctagc
    22921 ggaacggaag gaaacttcag ggacttaggg gggaataacc ccccgccgtc gcttgtcgtc
    22981 cttacgaacc gcgcggcagc caaaacattt ggcctccacg cgcgtctgta tctctggagg
    23041 acgctgatgg ctgcaactgt ttcgaaggtt tcgcccgttg tcgcgccaac cgctcgcaag
    23101 aatcgcgtcg cgcgcgttac ggccaagcgt ccgaagaagg aagcgtctga cgagcgcgag
    23161 cgcgatccag actccgagcc cgagctcgag agcgaagaag aagtcgtcga gaagccggcg
    23221 cgtgccgatg gcggcacgta cctctcgatg tacttcaaag acatggcgct gctcgacgtg
    23281 ctgcgccccg aggaagagtt caccgcggcg cgcgagatcg aggcgctcga gatcatgctg
    23341 tgggaagcgg tgctcgcgca tccgcaggcg atcgagcgcg tgctcgatgc gattcaagaa
    23401 gttcccgatt tcgtcatgcc gctcgaagcg aaaacgctgc gtcgcgcggc tgaaggccgc
    23461 gacagcaagg gattcgcgcg cgcagctgca cgcgccgcac gcaagctccg ctcgagcgat
    23521 gcagaccatc tcttcatcga tgccgcgctc ggcgcgatcg atcgcatgac gctgggcatc
    23581 ggcgtccgcg gcggtgacgt cgcgacactc ggcacgcgcg cgaaggtcgg cgagtggctg
    23641 cgcaaggtgc aatcggcgaa tcgcgccgcg aacgacgcgc gcaacgactt cgtcaaagcg
    23701 aaccttcgcc tcgtcgtcag catcgcgcgc cgcttcaacc acggccgcat ggcgctcgcc
    23761 gatctcatcc aagaaggcaa cctcggcctc atgaaggcgg tcgagcgcta cgactaccgc
    23821 cgcggcttcc gcttctcgac gtacgcgtcg tggtggattc gccacgcgat cagccgcgcg
    23881 ctcgccgaca agggccgcga agttcggctt cccgtgcaca tgatcgacgc gcagcatcgc
    23941 ctgacgaagg cgcgccgcca gctcaccggc aagctcggcc gtcagccgac gacggaagag
    24001 ctcgccgagg cgacgaacat gccgcccgac aaggtcgaaa agatgcgcag ctggctcctc
    24061 gagcaaagcg tgtcgatcga caagccggtc ggtgacgacg aaggccgcgt ccttggcgag
    24121 gtcctcgagg atcccgaccg cgaggtggcc ggtcccacgg acgacctcga gtgggtcgcg
    24181 ctcacgaccg aagttcagga gctgctgcgc gagctgcgcc cgatcgaggc cgatatcctt
    24241 cgccagcgct tcggcctcgg cacggagcag gagctgacgc tcaaagagat cggcgataag
    24301 tacaacctgt cccgcgagcg catccgccag ctccaggagc aggcgctcgc caagatgcgc
    24361 cgcgcgctat cccgacgaga tttgctgtaa ttccgggagt tgccgatgtg tcccgaagtt
    24421 aggtacgttc ggggcatgac ccgccgcctg ccgctactgc ccctgcttct cctcgtgctc
    24481 ggccttgctg cgtgcggcag cgaggacaag aagctcaagg tcaccggtat cgagccggag
    24541 aagggcgata tcgaaggcgg cacgtacgtc cgcatcaaag gcaatcgctt catcgccgat
    24601 ggtgcgcgca acgcgaaggt gtacttcggc tcgcgtcagg gcaccgtcgt tcgcttcgcg
    24661 agcgacagcg agctgatcgt cgaagcgccg ggtggcaagc cgaacgagaa ggtcgatgtc
    24721 ctcatcatct tcgagccggg cggcgagctg aagattccga acggcttcac gttcgtcgag
    24781 aagaacaacg aagcgccgtc cgtcgatcag ctcgatatca gcaaagtgaa gaagtagctc
    24841 cgagcggagc cgcttcgtta gcccgcgaag ttcaagcgga acagctgctg accgagcagc
    24901 acgaacgatt cgtgaccgac cggacgctcg ccgttcactt tgatgaacgt gccgttgctc
    24961 gaaccgaggt ctgcgaggaa cacgcgaccg tcgcgtagcg tgacgcgagc atgcagaccc
    25021 gagacgtagc cgtcgtcggg gaagttgatc tcgccgcgct cgcgaccgag cgtgacgctc
    25081 tcgccgagga gcgggaacgc agcgcccgtg acatcgcggc cgatgatgac ggtcagcttg
    25141 ccccagtagc cgggattcgg gctgcccatg agctcggtgc catcggccgt cggctcgggc
    25201 gtcggaatga tctcgaagcg aagcagctct tggccgatgc ggatgatctg gccgctctgc
    25261 agctcctctt cttgcgtcat cttcacgaac acgccgttga gcgaatcgag atcgcgaacg
    25321 accgcttgga cgccgcgaat gtcgagcatc gcgtggaccg gcgagagata gccgtcgttc
    25381 tcgaagacgg gaccgaagct gcgaccgagc ttgttctcgc ccgggcgaag atcgtgcgtg
    25441 ccgccttcgc tgccgtcggg acggatcagc gtcatcgaca ggcgcgagcg ctgcggttgc
    25501 ggcgccgcga acggtgcacc accgcccatc ggctgcggcg gacccggcat cggatttggt
    25561 gcggactgct cgtccatgcg gaaaccgcac gtgccgcaga agcggaacgt cggcggaaca
    25621 tcactgccgc acgacggaca gcgacgcatg ccgctcgcgg acaccggcgc gctcggcgcc
    25681 ggcggaaaac ctgcgtacgc gggaacgccg ggaggcggac ccatcggggg cggcgccatc
    25741 ggcgggccac ccatcgggcc accgcccatc ggtccgccac cgccgagcgg tcccgacgga
    25801 ggcatgccgg ccattccacc cgcgggcgga cgaccggggc ccgtcggacc accttggttc
    25861 ggcatgccgc cgctcggacc gcgcatcgga ccgccgccca ttggaccgcc gcccatcgga
    25921 ccgccgccca tcggacctcc gcccatcgga ccgccgccgc cgagtggcgc accgggaccc
    25981 gcggcgcctg gctccgccat catcgtcttc atcatgccca tgtcaccacc gccgcccggc
    26041 ttcggtgcag cgatgagctc agcaccgcat ccgaggcaga acttgtagtg gtcctggttc
    26101 tctttgccgc aacgattgca gacggtcatg gtggtccttt ggagtcgcct acacgatctc
    26161 gacgcgaagt agctgctgac cgaggaaaac gtaatcgccg tgcttgagca cgcgctcgcc
    26221 ggttacccgg acgaacgtgc cgttgcgcga ccccagatca gttacggata gcacgcctgc
    26281 agtcagctgg agctgggcat ggcgacccga gataaacggg tcgtcgggga agttgatgtc
    26341 gttgctctcg cgcccgatcg agaccgtttc ggtatcgacg cggaagaccc agccgctcga
    26401 gccgccgcgc agctgctgaa cgatttcgat cgttgcaggg cgcatcatgc tcgcggagaa
    26461 gtacgtgccc tcggtatcgg gaacgtcttc gaagcctttg gcctgcatca ccgagagcac
    26521 ttgttcgccg acgaggaccg tcgtgccggg ctcgatgcgc accgtgccgc tgatacgaac
    26581 gtagacgccg ttgagcgagc cttcgtcgcg cacgctcagc gcgccgttgg cgtaacggaa
    26641 gttgcagtga atcggcgaga gatacggatc atcgggaaac gagatcgggc attcgccgcg
    26701 acctgcgaga tgatcggtgc ccgcgaggct gaacgagacg ccgtcttcac cgtcgccgcg
    26761 gatcagcgtc agcttcgcgc gcgcttgctg catcggcccg ccgaagaaca gcgtgctgcg
    26821 tccgccttgc tttgcctgcg cgcccgtcga gccttgcggc attgctgctg ctgcggcgcc
    26881 gctcgtcggg ccaacgcgtg tctcgacgtt gaagtcttgc gcgacgacga cgcgcgttcc
    26941 gcagatctgg cagaacttga agttcggcgg gacgttcgtg ccgcacttgg tgcacgcgac
    27001 gccggctttt gcatcggcgc tcgccgttgc gaccgcacca ccattagctc ccggtggttt
    27061 actcgtacgt cgcggacgcg gatcgagtgc gaccggctgc ccgcaacgcg cacattgcgg
    27121 tactccgatt ggagtcaacg cgctacacgc gtcgcacacc aagc
//
LOCUS       DATLBZ010000067         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3780734, whole genome shotgun sequence.
ACCESSION   DATLBZ010000067 DATLBZ010000000
VERSION     DATLBZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3780734"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VMZ53_03310"
     CDS             complement(<1..828)
                     /locus_tag="VMZ53_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HUS27503.1"
                     /translation="MRGFVVVGLAISTGCGGADPCEGVSGTCIGLEEGASATDVQTAL
                     IEAQPNTTIAFGEGTFHFAVDLSLDVDNVTIAGAGMDATTLSFKGQQSGAQGFLVTGD
                     HFAIRDIAIEDTKGDALKIEGSDGVRIERVRVEWTGGPKETNGAYGLYPVQCKNVVID
                     GCVVKGASDAGVYVGQSDYIVVRNNRAEQNVAGIEIENSTRADVHDNTATNNTGGILV
                     FNLPGLQVENGSVTRVFHNQIFENNTVNFAPSGNIVGLVPTGTGMAILAAHQVEIFDN
                     "
     gene            914..1861
                     /gene="ligD"
                     /locus_tag="VMZ53_03315"
     CDS             914..1861
                     /gene="ligD"
                     /locus_tag="VMZ53_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HUS27504.1"
                     /translation="MKLKSLQLAQLVDEAPEGDDWLHEQKFDGYRILATLDHGAVELL
                     SRRFKDWTEQFPSVAEAVSHLKAKRAVLDGEVAVVMPDGRTNFQALQNAFGRRDANIV
                     YFAFDLLELDGEDIAKLPLDQRKERLKKLLGKKADGVIRYSDHVIGHGGKFFQLACKQ
                     GLEGIISKRRDKPYIPGRGSSWQKTKCILRQEFVIGGYTDPEGARTHVGALLVGYYED
                     GQLKYAGKVGTGFTMKTLVELKKALAPLETSKSPFTPEPPRAWTGPGRHWVTPTLVGE
                     VAFSEWTNDGRLRHPSFQGLRADKPPKDIIRERATHTNR"
     gene            complement(1880..>2528)
                     /locus_tag="VMZ53_03320"
     CDS             complement(1880..>2528)
                     /locus_tag="VMZ53_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HUS27505.1"
                     /translation="HTRVTDERGCELAKVTAFVTCEREGRPHLLLFEHPLAGVQLPAG
                     SIEDGEEPIAAALREVAEETGVSGLRDATELASARTELAPGSAVLLARLVPRTEPHPD
                     ALSCGAGLSRGLTVEVLDRWTDFARIRYDELDRRRVPPMLVARTVGWVQAHLLGGVVV
                     RHYVHLRHDGSGPARWTQDADGHRFAPFWAPLVPRPAICEAQEAWLDAVYEQLLL"
BASE COUNT          488 a          897 c          737 g          406 t
ORIGIN      
        1 gttgtcgaag atctcgactt ggtgcgcggc gaggatcgcc atgccggtgc ccgtcggcac
       61 gagcccgacg atgttgccgc tcggcgcgaa gttcacggtg ttgttctcga agatctgatt
      121 gtggaacacg cgcgtgaccg acccattctc gacctgcagg ccgggcaggt tgaagacgag
      181 gatgccgccg gtgttgttcg tcgcggtgtt gtcatgcaca tcggcgcgcg tcgagttctc
      241 gatctcgatg ccggcgacgt tctgctcggc gcgattgttc cgcacgacga tgtagtcgct
      301 ctggccgacg tacacgcccg catccgacgc gcccttcacg acgcagccat cgatcacgac
      361 gttcttgcac tgcacgggat agagcccgta cgcgccgttc gtctccttcg gcccgccggt
      421 ccactcgacg cggacgcgct cgatgcgcac gccatcactg ccttcgatct tcagcgcgtc
      481 gcccttcgtg tcttcgatcg cgatgtcgcg aatcgcgaag tggtcgccgg tcacgagaaa
      541 tccctgcgcg cccgactgct gccccttgaa cgacagcgtc gtcgcatcca tgcccgcgcc
      601 ggcgatcgtc acgttgtcga cgtcgagcga gaggtcgacc gcaaaatgaa acgtcccctc
      661 gccaaacgcg atcgtcgtgt tcggctgcgc ctcgatgagc gccgtctgga catccgtggc
      721 actcgcgccc tcctcgagcc cgatgcaggt tccactgacg ccttcgcatg gatccgcacc
      781 gccacatccc gtcgaaatcg cgaggcccac cactacgaat ccgcgcatgg gcggcaagtt
      841 cgccaaaatt ggtcagaccg tcaagacaat tggtctgtcc actaattgtc caagccttgc
      901 aagaagttca ggcgtgaagc tcaagtcgct ccaactcgcg cagctcgtag acgaggcccc
      961 cgaaggtgac gactggctgc acgaacagaa gttcgacggc taccggatcc tggccacgct
     1021 cgatcacggc gcagtcgagc tcctctcgcg ccgcttcaaa gactggacag agcaattccc
     1081 gtctgtcgcc gaagcggtct cgcacctcaa agccaagcgc gcggtcctcg atggcgaggt
     1141 cgcggtcgtg atgcccgacg gccgcacgaa cttccaagcc ctccaaaacg cgttcggccg
     1201 ccgcgacgcg aacatcgtct acttcgcgtt cgacctcctc gagctcgatg gcgaagacat
     1261 cgcaaagctg ccgctcgacc aacgtaaaga gcgcctcaaa aagctgctcg gcaaaaaagc
     1321 cgacggcgtc atccgctaca gcgatcacgt gatcggccac ggcggcaaat tcttccagct
     1381 cgcgtgcaag caaggcctcg aaggcatcat ctccaagcgt cgcgacaagc cgtacatccc
     1441 aggtcgcggc tcgtcgtggc agaaaacgaa atgcatcctg cgccaagagt tcgtgatcgg
     1501 cggctacacc gatcccgaag gtgcccgcac tcacgtgggc gcgttgctcg tcggctacta
     1561 cgaagacggc cagctcaagt acgcaggcaa agtcggcacc ggcttcacga tgaagacact
     1621 cgtcgagctc aagaaagcgc tcgcgccgct cgagacatcg aagtcgccat tcacgcccga
     1681 gccaccgcgc gcatggaccg gcccaggccg ccactgggtc accccaacgc tcgtcggcga
     1741 ggtcgcattc tccgagtgga cgaacgacgg ccgccttcgc cacccatcgt tccaaggcct
     1801 gcgcgccgac aagccgccga aagacatcat ccgcgagcgc gcgacccaca ccaaccgctg
     1861 actgccgccg gcttccaacc tagagaagta gctgctcgta gacggcgtcc agccacgcct
     1921 cctgcgcctc gcagatcgcg ggcctcggaa caagcggcgc ccaaaacgga gcgaagcgat
     1981 gcccatcggc gtcctgcgtc caacgcgccg gcccgctgcc atcatgacga aggtgcacgt
     2041 aatggcgaac gacgacgccg ccgagcaaat gcgcctgcac ccagcccacc gtccgcgcga
     2101 cgagcatcgg cggcacacgc ctgcgatcga gctcgtcgta tcgaatgcgc gcaaagtcgg
     2161 tccaacgatc cagaacctcg acggtgagcc cgcggctcaa cccagcgccg cacgaaagcg
     2221 catcagggtg aggctcggtg cgcgggacca agcgcgccag cagcacggct gatccaggcg
     2281 ccagctccgt gcgcgcgctc gcaagctcgg ttgcatcgcg aagtccagac acacccgtct
     2341 cctcggccac ctctcgcagc gcggcggcga tcggctcctc gccatcctcg atgctccccg
     2401 cagggagctg cacgcctgcg agtggatgct cgaacaagag cagatgaggg cgcccctcgc
     2461 gctcgcacgt aacaaacgcc gtcaccttcg cgagctcgca gccgcgctcg tcggtcacgc
     2521 gtgtatgt
//
LOCUS       DATLBZ010000068         3343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4525424, whole genome shotgun sequence.
ACCESSION   DATLBZ010000068 DATLBZ010000000
VERSION     DATLBZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4525424"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VMZ53_03325"
     CDS             complement(<1..514)
                     /locus_tag="VMZ53_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27506.1"
                     /translation="MAKCLLAVVLFLSGCDLLFQIDELHGGADAAPGVEVDSVDAAVQ
                     TTQPDAVSNCTVEEFASLGSQWTPFSNPPDTSVAINGALSITLHNASNEHGGLNTAVR
                     DFTGQSIEVDVIAVPDAASETYIDWWRGDDWYSIAVDHGQLSYGFSVNGTDSTVEIPY
                     VPQQHRGFRIA"
     gene            complement(617..1405)
                     /locus_tag="VMZ53_03330"
     CDS             complement(617..1405)
                     /locus_tag="VMZ53_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27507.1"
                     /translation="MATLPFDRLPDDKPGGVFSVVEGRDGWLLVIAHDSAFHRLLGEG
                     EAMRLSARGTALFFDCETSAMTSRLVAFESGKQAWAVDSSGPNDTKAPPEIEAIIAAG
                     REKQIAEDARHAEDSDELPVSYEWDAVLEAGEILTGYHAEQRSDLRTLCRLAPRITEA
                     ERLLSVTGRAQVIEPEAKAVIEALAALEVGTSVKLAVRRYGSRCQLIAAYEGGQLCVD
                     VLEGPRYRARRSDGTAIQREDLEAIFLAFLREGERSPSFAWRRV"
     gene            1484..2857
                     /locus_tag="VMZ53_03335"
     CDS             1484..2857
                     /locus_tag="VMZ53_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27508.1"
                     /translation="MRNGIVLVVAIVAACKGDRGKVGRGSSTAEPSGPKVHVSATPLP
                     VVGTDARLLPANAPVPAHELVIDATGRIAALGVEENWPEVLVQADTQDPLTGESIVTY
                     GANVHRKLMDDYDKKRMADADAAEEAEVKRRIEEGEQESAIRHEMATAHYEPEWGHLG
                     PYLFADKPYSMTPIIGDGVAVREGAPSRMPRLMADTTDTNPPEPFLFVVAANAPAHVL
                     VDAMIATSGGLIAVAAPHGVRALRLSFKHIEPNDAHFWEVPWAPWIEVRLGAGTIDVE
                     AVPGTPVRLTGAVDGETLQDTIAKLRAQRPGLAADHSVDVLVDDRADAQHLVTVLAAL
                     DLANANEISLGLAPRPADGDVMRGKPLSVIPVPAIEAERGEGALGTARDLIVPRMPAI
                     RKCFADAPAKTAVVIRTDRNDKTRAITSRIEGKPSPVATCVFAELEDSFNHQTAAFKV
                     RFARIES"
     gene            2867..>3343
                     /locus_tag="VMZ53_03340"
     CDS             2867..>3343
                     /locus_tag="VMZ53_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HUS27509.1"
                     /translation="MITLYGFKRIFPEGIGETKDLRAQWVLEETGLPYRVHGLDHGAG
                     DLDSESYSRISPFNQVPVIDDDGFVISESAAIVLYVAEKAGKLIPKDFQGRTRVMQWC
                     FAAVSTVAPTLTALDLLGIFDPDKTAPRLHTETDKLADRWFHGVERRLEGREWIATD"
BASE COUNT          598 a         1139 c         1075 g          531 t
ORIGIN      
        1 gcgcgatccg aaagccacga tgctgctgcg gtacgtacgg aatctcgacg gtgctgtccg
       61 ttccgttcac ggagaagccg tacgacagct ggccgtgatc gaccgcgatc gagtaccagt
      121 cgtcgccgcg ccaccagtcg atgtacgtct cgctcgccgc atccggcacg gcgatcacgt
      181 cgacttcgat cgactggccc gtgaagtcac gcacggcggt gttgagccca ccatgttcgt
      241 tcgatgcgtt gtgcagcgtg atcgacaacg cgccgttgat cgcaacggac gtatcgggcg
      301 gattggagaa cggcgtccac tggctaccga ggctcgcgaa ctcttcgacg gtgcaattcg
      361 aaaccgcatc cggctgggtc gtctgaacag cggcgtcgac ggagtcgacc tcgacgcctg
      421 gcgccgcgtc cgcaccaccg tgcagctcgt cgatctggaa caggaggtcg cacccgctca
      481 ggaacagaac aaccgcgagg aggcacttcg ccatcagcgc ggacgagtgc acgtcctgtg
      541 cagagccaag gcccgcgaaa ctacgggagt cgcattccgt cgctgcgtcc cggaggatgc
      601 gccggtgcaa ccccgtctag acgcggcgcc acgcaaagct cggcgagcgc tcaccctcgc
      661 gcaggaacgc caagaagatc gcctcgagat cctcgcgctg gatcgccgtg ccatccgagc
      721 ggcgcgcgcg ataacgcggc ccctcgagca cgtcgacgca gagctggccg ccttcgtacg
      781 ccgcgatcag ctggcatctc gatccgtagc gacgcacggc gagcttcacc gacgtcccga
      841 cctcgagcgc tgccagcgct tcgatcacgg ctttggcttc gggctcgatc acttgcgccc
      901 gacccgtgac cgagagcagc ctctctgctt cggtgatgcg aggcgccagg cggcagagcg
      961 tccgcaggtc gctcctctgc tcggcgtgat agccggtcaa gatctccccg gcctcgagca
     1021 cggcgtccca ctcgtacgac accggcagct cgtctgaatc ttcggcgtga cgcgcgtcct
     1081 cggcgatctg cttctcgcga ccggcagcga tgatcgcttc tatctccggt ggcgccttcg
     1141 tgtcgttcgg ccccgagctg tcgaccgccc acgcctgctt gccgctctcg aacgcaacga
     1201 gcctcgacgt catcgcgctg gtctcgcagt cgaagaacag cgcggtgccg cgcgcggaca
     1261 ggcgcatcgc ctcgccttcg ccgagcagcc gatgaaacgc ggaatcgtgt gcgatgacga
     1321 gcagccaccc atcgcggcct tcgacgacgg agaacacacc acccggcttg tcatcgggca
     1381 ggcgatcgaa gggcagcgtg gccaccagcc catccacccg taggccacgg actgcgagcc
     1441 agcgtgcgcg tgtccccatg ccgcgtgagt gtagagtgcg agcgtgcgta acggaatcgt
     1501 cctggtggta gcgatcgtcg cggcgtgcaa gggcgatcgc gggaaggtcg ggcgtggctc
     1561 gtcgacggcg gagccatccg ggccgaaggt ccacgtgtca gcgacgccgc tcccggtcgt
     1621 cggaaccgac gcgcgcctat tgccggcgaa cgcgcctgtg ccggcgcacg agctcgtcat
     1681 cgatgctact ggccgtatcg cggcgctcgg cgtcgaggag aactggcccg aggtgctcgt
     1741 gcaggccgac acccaggacc cgctcaccgg cgagagcatc gtgacgtacg gcgcgaacgt
     1801 ccaccgcaag ctgatggacg attacgacaa gaagcgaatg gccgacgcgg atgcggccga
     1861 ggaagcggag gtcaagcgcc gcatcgagga aggcgaacag gagtccgcga ttcgccacga
     1921 gatggcgacg gctcactacg aaccggagtg gggccacctc gggccgtacc tgttcgccga
     1981 caagccgtac tcgatgacgc cgatcatcgg cgatggcgtc gccgttcgcg aaggtgctcc
     2041 atcacggatg ccgcggctca tggcggatac gacggacacg aatccacctg aaccgttctt
     2101 gttcgtcgtc gcggcgaacg cgcctgccca cgtcctcgtc gatgcaatga tcgcaacgag
     2161 cggcggtctg atcgcagtcg ccgcgccgca cggcgttcgt gcactgcgcc tgagcttcaa
     2221 gcacatcgag ccgaacgacg cgcacttctg ggaggtgccg tgggcgccgt ggatcgaagt
     2281 ccggctcggt gcaggcacga tcgatgtcga agccgtaccc ggcacgccgg tcaggctcac
     2341 cggtgccgtc gatggagaga cgctgcagga tacgattgcg aagctgcgcg cgcagcggcc
     2401 tgggctcgcc gccgatcatt cggtcgatgt gctcgtcgat gatcgggcgg acgcgcaaca
     2461 cctggtcact gtcctcgcag cgctcgatct agcgaacgcg aacgagatca gcctcggact
     2521 cgcgccgcgt ccggccgacg gtgacgtcat gcgcggcaag ccgctatccg tgatcccggt
     2581 ccctgcgatc gaggccgaac gtggcgaagg agcacttggc accgctcgcg atctgatcgt
     2641 gccgcgcatg cccgcgatcc gaaagtgctt cgccgatgcg cccgcgaaga ctgcggtcgt
     2701 gatccgcacc gatcgcaatg acaagacgcg agcgatcaca tcgcgaatcg aaggcaagcc
     2761 ctcaccggtt gcgacgtgcg tgttcgccga gctcgaggat tccttcaatc atcaaaccgc
     2821 ggctttcaag gttcgcttcg cgcgcatcga gtcgtagact gcgcacgtga tcacgctgta
     2881 cggcttcaag cgaatcttcc cggagggcat cggcgagacg aaagacctgc gcgcgcagtg
     2941 ggtgctcgaa gagacgggcc ttccctaccg cgttcacggc ctcgaccacg gcgccggcga
     3001 cctcgacagc gagagctaca gccgcatcag cccgttcaac caagtgccgg tcatcgacga
     3061 cgatggcttc gtcatctccg agtcggcagc gatcgtcctc tacgtcgccg agaaagccgg
     3121 caagctgatc ccgaaggatt tccaagggcg cacgcgcgtc atgcagtggt gcttcgccgc
     3181 ggtcagcacg gtagcgccca cgctcaccgc gctcgatctc ctcggcattt tcgatcccga
     3241 caagactgca ccaaggctcc acaccgaaac cgacaagctc gccgaccgct ggttccacgg
     3301 cgtcgagcgt cgcctcgagg gccgcgaatg gatcgcgacc gac
//
LOCUS       DATLBZ010000069         8308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8994807, whole genome shotgun sequence.
ACCESSION   DATLBZ010000069 DATLBZ010000000
VERSION     DATLBZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8994807"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VMZ53_03345"
     CDS             <1..601
                     /locus_tag="VMZ53_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HUS27510.1"
                     /translation="ERMLEPRYQIAVTRCVTCKRGWQHGASTTPLSPAEVERALCDCV
                     DIGNVDDEPARAKRSIAKALKRHVLHRDGYRCRVPGCGATANVDCHHIIFREDGGANE
                     APNLIALCEGHHLALHEGSLVIEGDATNARFTRRAQNNFKIATRAVECAAGLRARGVA
                     KELVKAAVDATRTHVGQHDLTTRQWIDIALTKLPAARQP"
     gene            683..2008
                     /gene="eat"
                     /locus_tag="VMZ53_03350"
     CDS             683..2008
                     /gene="eat"
                     /locus_tag="VMZ53_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HUS27511.1"
                     /translation="MTQLQRTLGPVLIWGLGVGYVISGMYFGWNLGLPEGGPYGMLVA
                     TGIVTILYISFVLGYAELACALPRAGGAFVYTSRAFGPDVGFLGGIAQLVEYMLAPPA
                     VAFAIGSYINQAAPDVPIIGVAVIAYIVFTLVNIWGVKLSVGFELVLTVIAVIELCVF
                     GVVVLPKFSWAHFSADPLPHGWGKGVLAALPFAIWFYLAIEGIANVAEEARNPQRDLP
                     RGFLWAMATLVGLTAITLFGAVGADGWHSVVYPDPANTSVTSDSPLPLAVSHVVSRDS
                     PLFVVLTGIGLVGLVASFHGILIAGSRALLEFGRAGYAPKLLGIINPRTRTPVFALIG
                     NFVVGLITLATGKTGDIILIAVMGALTLYILSSAAVIRLRMREPALDRPYHAPLYPVT
                     PVIACGLSAICLGSMAWAHPKLALVYLAILGGTWGLFRAFVPPEKRTTF"
     gene            2021..3325
                     /locus_tag="VMZ53_03355"
     CDS             2021..3325
                     /locus_tag="VMZ53_03355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUS27512.1"
                     /translation="MRNVLLALLLLGAAVACEQNKSKLDGVANTSSSSKTGDAIDIDS
                     KDILGRKEAATNVWVKHVLIAWKDLDKVYQGRMDPRATNRSNEEAVKLARDIEKQLKA
                     SPDKIDELVKTHSEDPGSASGEPYQVKADTQFVPEFKQLALRLKEKEVGIVKSKYGYH
                     IMERVAAPPLDPLESADILARTAEPGMVHVQHVLIGWKDTPAAKAGRVDEKAKNRDKA
                     EADKMAQEVLGKAKAGEDFKALMKQYSEDPGSKDDGRVYPVAPDAPMVEPFKDMSLRL
                     KMGEVGMVKSPFGWHVIKRVEPPPPPPPPAPEKPDALDSADILKREPVTKTAEVKHIL
                     LGWKDQAGGGDDPRGQKRDRAALEKQVKELVARLKKGEKIEPLMKEFSEDPGSAQSGS
                     SYPVEEKAALVEPFKQLSLRLNVNEVGVVKSQFGIHIIQRVK"
     gene            complement(3306..3605)
                     /locus_tag="VMZ53_03360"
     CDS             complement(3306..3605)
                     /locus_tag="VMZ53_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27513.1"
                     /translation="MPTIATQRILRAIVDERRRALSRSLHGQHHRPPPIENRLAQLKS
                     QVEAYRQQAIVLNAKLAALGDDFQAMRETLSKSSAQIDLGVAGIVGDAPVTSRVE"
     gene            complement(3692..5467)
                     /locus_tag="VMZ53_03365"
     CDS             complement(3692..5467)
                     /locus_tag="VMZ53_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS27514.1"
                     /translation="MKRLAVVLLASCTGSVGELPSPTPPPTWGVPISGGNLLVTRDGS
                     RAVIADPDRDRVMLVQLANGTTTELPLPARSEPGRLVEDGAGRVHIALRGSGQLLTLT
                     DSGSEQRAVCPEPRGVAYDAASDLVHVACTTGELVSLPAAGGPAVRTLHLDRDLRDVV
                     VRGSDLVVTRFRSAELLTIDASGAITTRTTPPLVHRLDIVGGGELTPPNIPPTKSDAP
                     PTVAWRTIALPDGRIVMSHQRKRSKELQVTGGGYVDTECGDGMIESAITLQRPDGTLS
                     AVSPILHGALPVDVAANAAGTELAFVTAGPQRVAVVSASALDSHDDDECGDDQDDNVS
                     ARLLGDDLGTPTSVAYAPDGTLAIYYPELPALVIHTSPASADSARTIRLPGPLGYDSG
                     RALFHRQTNAALACASCHPEGRDDGQVWDFVAGPRRTQNLSGGILARAPFHWNGDLPD
                     LGTLMTTVFEQRMAAGATTHSERLSLGPWLDRIPAPRAAITDAAAVARGQALFVAPEQ
                     ACATCHMGESYTNNVLADVGTGGQFKVPSLIGIGARAPYMHDGCATTLRERFSAECGG
                     GDSHGRIAHLTESQISDLIAYLESL"
     gene            complement(5464..6405)
                     /locus_tag="VMZ53_03370"
     CDS             complement(5464..6405)
                     /locus_tag="VMZ53_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUS27515.1"
                     /translation="MLATLPAACVGSVEESWQDDCTIHRTKTFEVDLPADPPLDLRVE
                     SCRLDADACRAMCNMLMSRAGLSAGTCAVEFHDQAVKAVSAYTEYIDTGHCQSSEGRR
                     PAGLVIPRCIEANSAIGAWLAHAAWLEAASIPAFIYLARELDMHGAPRGLARMALASA
                     RDEIRHAHMMNALAARFGGKPPIADVDLPKPRSLEDMAIENAIEGCVRETWGAIVAMW
                     QAERASDRELRNAFRSISGDEARHSALAWAVDNWLQTRLDDAAKKRVIEARHAAVREL
                     FEGTDAEVNTVLGLPFGADAHRLVARTERALWQGGLS"
     gene            6590..7996
                     /locus_tag="VMZ53_03375"
     CDS             6590..7996
                     /locus_tag="VMZ53_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS27516.1"
                     /translation="MKRLAVALVAVVGMSTVAQAGVEIGGTAGLHMFSESNSLGTPNT
                     MIHQANSAMFGLRFGFYFSSMLGLELEGGLIPTESAGSNVVFDIYDATARANLVAQFR
                     TSDSANTIIPFVLVGGGETRVIKIGTSDDSLFKKDDMRGSVYLGAGAKYRAGGGWGVR
                     VDVRGIFVHDNTGAAFTNDVEILASLYREWGRKKTVKVEDKPKTGADGDSDGIEDATD
                     KCPTEAEDKDDFQDDDGCPDKDNDADGVADDTDKCKGEAEDKDNYQDDDGCPELDNDS
                     DGVPDASDKCATEAETKNGFDDEDGCPDEVPPELTKVIGPITGVSFKANSADLAPASN
                     KALDAVAATLAKFPTVKVEIGAHSDDQALKAGGKFADNDALTAAQAEAVKAYLVQKGA
                     AEGQITVKGYGSTKPMNDPAGLKGAKLAAARKINRRVELTLVVPEPASTPTETKPVET
                     PTAPPAEGGAKTEEPKLP"
BASE COUNT         1466 a         2644 c         2837 g         1361 t
ORIGIN      
        1 cgaacgcatg ctcgagccgc gttatcagat tgcggtgacc cggtgcgtga cgtgtaagcg
       61 aggctggcag cacggggcga gcacgacgcc tttgtcgccc gcggaggtcg aacgggcgct
      121 gtgcgactgc gtcgatatcg ggaatgtcga cgacgagccc gcgcgagcga agcgaagtat
      181 tgcgaaggcg ctgaagcgcc atgtgctgca tcgcgatgga tatcggtgtc gcgtgcccgg
      241 atgcggagcg acggcgaatg tcgattgcca ccacatcatc tttcgagaag acggcggggc
      301 gaatgaagcg ccgaacttga tcgcgctctg cgaggggcat cacctcgcgc tgcacgaagg
      361 ctcgctcgtg atcgagggcg atgcgacgaa tgcgcgtttc acgcgccgtg cgcagaacaa
      421 cttcaagatc gctacgcgtg ccgtggaatg tgcagctggc ctgcgtgcga ggggtgtcgc
      481 gaaggagctc gtcaaggcgg cggtcgacgc gactcgcacc cacgtgggtc aacacgatct
      541 gacgacgcgg cagtggatcg acatcgcgct caccaagctt ccggcggcaa ggcaaccgtg
      601 aagtcgcccg gcgagccgcg acttgccact ctccaccacc atcgccattc gcgcggcgtt
      661 tcagcggtga gagactagag ccgtgacgca actacaacgc accttgggac cggtcctgat
      721 ttggggcctc ggcgttggct acgtcatctc cggcatgtac ttcggctgga acctcggact
      781 gcccgagggc ggcccgtacg gaatgctggt cgcgacgggc atcgtcacga tcctctacat
      841 ctcgttcgtg ctcggctatg ccgagctcgc gtgcgcgtta ccccgagcgg gcggggcctt
      901 cgtctacacg agccgggcat ttgggcccga cgtcggattt ttgggcggca tcgcgcagct
      961 cgtcgagtac atgctcgcgc cgccggcggt tgcgtttgcc attgggagct acatcaacca
     1021 ggcggcgccg gatgtgccca tcatcggcgt cgcggtcatc gcgtacatcg tctttacgct
     1081 cgtcaacatc tggggcgtga agctcagcgt cgggttcgag ctcgtgctca ccgtgatcgc
     1141 ggtgatcgag ctgtgcgtgt ttggcgtcgt cgtgttgccg aagttttcgt gggcgcactt
     1201 ttctgcggat ccgttgccgc atgggtgggg caagggcgtg ctcgcggcgc tgccctttgc
     1261 gatttggttt tatctcgcga tcgagggcat cgcgaatgtg gcggaggagg cgcgcaatcc
     1321 gcagcgcgat ctgccgcgcg gcttcttgtg ggcgatggcc acgctcgtcg ggctgaccgc
     1381 catcacgctc ttcggcgcag tgggcgcgga cggctggcac tccgtcgtct atcccgatcc
     1441 ggcgaacacg agcgtgacca gtgactcacc tctgccactc gcggtgagcc acgtcgtgtc
     1501 acgcgatagc ccactgttcg tcgtcctcac gggcatcggg ctcgtcgggc tcgtcgcgag
     1561 ctttcacggc attctgattg cgggcagccg cgcgctgctg gagttcggcc gtgccggtta
     1621 tgcgccgaag ctcctcggca tcatcaatcc gcgcacgcgc acgccagttt tcgccctgat
     1681 cggcaacttc gtggtcggcc tcatcacgct cgccacgggc aagaccggcg acatcatctt
     1741 gatcgcggtg atgggcgcac tcacgctcta tattctgtcg tcggcggcgg tcatccggct
     1801 gcgcatgcgc gagcccgcgc tcgaccggcc gtatcacgca ccgctttatc cggtgacacc
     1861 cgtgattgcg tgcggcctca gcgcaatttg ccttggcagc atggcgtggg cgcacccgaa
     1921 gctcgcgctc gtgtatctgg cgatcttggg cggtacgtgg ggcttgtttc gggcgttcgt
     1981 gccacccgag aagcgcacaa cgttctagac tgcgcgcctt atgcgcaacg tcttattggc
     2041 gcttctgctg ctcggtgcgg cggtggcctg tgaacagaac aagagcaagc tcgatggcgt
     2101 cgccaacacg tcgtcgtcgt cgaagaccgg cgacgcgatc gacatcgaca gcaaggacat
     2161 ccttggccgc aaggaggctg cgacgaacgt gtgggtcaag catgtcctga tcgcctggaa
     2221 ggatctcgac aaggtctatc aaggccgcat ggacccgcgc gccacgaacc gctcgaacga
     2281 agaggcggtc aagctcgcgc gcgatatcga gaagcagctc aaggcgagtc ccgacaagat
     2341 cgacgagctc gtgaagacgc actccgagga tccggggtca gcgtcgggcg agccgtatca
     2401 ggtgaaggcc gacacgcagt tcgttcccga gttcaagcag ctcgcgctga ggctcaagga
     2461 gaaggaagtc ggcatcgtga agtcgaagta cggctatcac atcatggagc gcgtcgcggc
     2521 cccgccgctg gatccgctcg agtcggcgga catcctcgcg cggactgcgg agcccggcat
     2581 ggtgcacgtg cagcacgtgc tcatcggctg gaaggacacg cccgcggcga aagcgggtcg
     2641 cgtcgacgag aaggcgaaga accgcgacaa ggcggaagcc gacaagatgg cgcaagaggt
     2701 tctcggcaag gcgaaggccg gcgaggactt caaggcgctc atgaagcagt actcggagga
     2761 tccgggcagc aaggatgacg gccgcgtgta tccggtcgcg ccggatgcgc cgatggtcga
     2821 gccgttcaag gacatgtcgc tgcgtctgaa gatgggcgag gtcggcatgg tgaagtcgcc
     2881 gtttggctgg cacgtgatca agcgcgtcga gccgccgccg ccaccgccgc cacccgcgcc
     2941 ggagaagccc gatgcgctcg actccgcaga catcttgaag cgcgagccgg tgacgaagac
     3001 cgccgaggtg aagcacatcc ttctcggctg gaaggatcag gcgggcggcg gtgacgatcc
     3061 gcgcggccag aagcgcgatc gcgcggcgct cgagaagcag gtgaaggagc tcgtcgcgcg
     3121 cctcaagaag ggcgagaaga tcgagccgct gatgaaggag ttctccgagg atccgggctc
     3181 cgcgcagagc ggcagcagtt atcccgtcga ggagaaggcg gcgctcgtcg agccgttcaa
     3241 gcagctctcg ctacgcctca atgtgaacga agttggcgtc gtgaagagcc agttcggtat
     3301 tcacatcatt caacgcgtga agtaacgggc gcgtcgccga cgatcccggc cacgccgaga
     3361 tcgatctgcg cgctcgattt cgagagcgtc tcgcgcatcg cctggaagtc gtcgccgagc
     3421 gcagcgagct tggcgttgag cacgatcgct tgctggcgat acgcttcgac ttggctcttg
     3481 agctgagcga gccggttctc gatcggcggc ggccgatgat gttggccgtg caaggatcgc
     3541 gagagagcgc gacgtcgctc gtcgacgatc gcacgcagga tccgctgggt cgcgatggtc
     3601 ggcatgggac aaggctggtg ccgatccgcg ttcatcgcca ctttctgact gtcttcaaac
     3661 gtaatcgcgt gatgacatcg cgcgcagccg ctcacaatga ttcgagatac gcgatcagat
     3721 cgctaatctg cgattccgtg aggtgtgcga tgcgaccgtg cgagtcgccg ccgccacact
     3781 ctgcggagaa gcgctcccgc agcgtcgttg cgcaaccatc gtgcatgtag ggcgcgcgag
     3841 caccgatgcc gatgaggctc ggcactttga actgaccacc ggtgccgacg tcggcgagca
     3901 cgttgttcgt gtacgactcg cccatgtggc acgtcgcgca tgcttgctcg ggtgcgacga
     3961 acagtgcctg accacgtgcg acggcggctg catccgtgat cgcggcgcgc ggcgcgggaa
     4021 tgcgatcgag ccacgggcca agcgagagcc gctcgctatg cgtcgtggcg cctgcggcca
     4081 tgcgctgttc gaagacggtg gtcatcagcg tgccgaggtc ggggaggtca ccgttccagt
     4141 gaaatggtgc gcgcgcgagg atgccgcccg agagattctg cgtgcggcgc ggaccggcga
     4201 cgaagtccca aacctgtccg tcgtcgcgac cttcgggatg acacgacgcg cacgcgagcg
     4261 ccgcgttcgt ctggcgatga aacagcgcgc gtcccgagtc gtagccgagt ggaccgggca
     4321 ggcgaatggt gcgcgccgaa tccgcggatg ccggcgacgt gtggatgacg agtgcgggga
     4381 gctccgggta gtagatggcg agcgtgccgt cgggggcgta cgcgacggac gtcggcgtgc
     4441 cgaggtcatc gccgagcaag cgcgcgctga cgttgtcgtc ttggtcgtcg ccgcattcgt
     4501 cgtcgtcgtg cgagtcgagc gcggatgccg atacgacggc gacgcgctgc gggccagcgg
     4561 tgacaaatgc gagctcggtg ccggccgcat tcgcggcgac gtcgacgggc agcgcgccgt
     4621 gcaagatggg cgagaccgcc gacagcgtgc cgtccggacg ctgcagcgtg atcgcgctct
     4681 cgatcatgcc gtcaccacac tcggtgtcga catagccgcc gcccgtgact tgcagctcct
     4741 tcgagcgctt gcgttgatgc gacatcacga tgcggccgtc gggcagcgcg atcgtgcgcc
     4801 atgcaaccgt cggtggggcg tcgctcttcg tcgggggaat gttgggcggc gttagctcgc
     4861 caccgcccac gatgtcgagg cgatgcacga gcggaggcgt ggtgcgcgtc gtgattgcac
     4921 cactcgcatc gatcgtgagc agctccgcgc tgcggaagcg cgtgacgacg aggtccgaac
     4981 cgcggacgac gacatcgcgc aaatcgcgat cgagatgcag cgtgcgcacg gcggggccgc
     5041 ccgcggcagg aagactgacg agctcgcccg tcgtgcacgc gacgtgaacg agatcgctgg
     5101 cagcgtcgta cgcgacgccg cgaggctcgg gacacacggc gcgctgctcg ctacccgaat
     5161 cggtgagcgt cagcagctgg ccactgccgc gcagcgcgat gtgaacgcgg cccgcgccat
     5221 cttcgacgag gcggcctggc tcactgcgag ctggaagcgg aagctctgtc gtcgtgccgt
     5281 tcgcgagctg cacgagcatc acgcgatcgc ggtcggggtc tgcgatgacg gcccgcgagc
     5341 cgtcgcgcgt gacgagcaag ttgccgccgc tgatcggcac gccccacgtg ggaggcgggg
     5401 tcggagatgg cagctcaccg acgctgccgg tgcacgaagc gagaaggacc acggcgaggc
     5461 gtttcacgag agacctcctt gccagagcgc gcgttcggtt cgcgcaacca gccgatgcgc
     5521 atcggcgccg aacggcagac cgagcaccgt gttgacctcg gcgtccgtgc cttcaaatag
     5581 ctcgcgcacc gcagcgtggc gcgcttcgat cacgcgcttc ttcgcggcgt cgtcgagacg
     5641 cgtctgtagc cagttgtcca cggcccacgc gagcgccgag tggcgcgctt cgtcgccgct
     5701 gatcgagcgg aatgcattgc gaagctcgcg atcgctcgcg cgctcggctt gccacatcgc
     5761 gacgatcgcg ccccacgtct cgcgcacgca gccttcgatc gcgttctcga tcgccatgtc
     5821 ctcgagcgag cgtggcttcg gcagatcgac gtcggcgatc ggcggcttgc cgccaaatcg
     5881 tgcggccagc gcgttcatca tgtgggcgtg gcgaatctcg tcgcgtgcag aggcgagcgc
     5941 catacgcgcg aggccgcgag gggcgccgtg catgtcgagc tcgcgcgcga ggtagatgaa
     6001 tgccggaatc gatgcggctt cgagccacgc agcgtgcgcg agccacgcgc cgatcgcgct
     6061 gttggcctcg atgcagcgag gtatgacgag gcctgcgggg cggcggccct cggacgactg
     6121 gcagtggccc gtgtcgatgt actccgtgta agcggagacc gctttgaccg cttggtcatg
     6181 gaactcgacc gcgcaggtac cggccgagag cccggctcgc gacatcagca tgttgcacat
     6241 cgcccggcac gcgtcggcat cgagccggca gctctcgacc cgcaaatcga gcggcgggtc
     6301 ggccggcaga tcgacctcga acgtcttcgt tcgatggatc gtgcagtcgt cctgccacga
     6361 ctcctcgacg gagccgacgc aggcagcggg aagggtcgcc agcacgatcg attgcaacac
     6421 ccgtcggagc tcgcggaact gcatggccag ccgcaaagtg caacggccat tccgcgagat
     6481 aaccgcctgg aacgcctagc gtcagatcac agtattctga ggcgatccgc agagattttc
     6541 ggtgggtttt gcttgcgccc agggggcgta tcgaacgaaa acgcccgcca tgaagcgttt
     6601 agccgtagcc ctagtcgcgg tcgtggggat gtcgacagtt gcccaggcgg gtgtcgagat
     6661 cggtggcacc gcgggtctgc acatgttcag cgagtcgaac tcgctcggaa cgccgaacac
     6721 gatgattcac caggcgaact ccgcgatgtt cggcctgcgc ttcggcttct acttcagcag
     6781 catgctcggg ctggagctcg agggcggcct gattccgacc gagtcggccg gctcgaacgt
     6841 cgtgttcgac atctacgacg caacggcgcg cgccaacctc gtcgcgcagt tccgcacgtc
     6901 cgactccgcg aacacgatca tcccgttcgt cctggtcggt ggcggcgaga cgcgcgtcat
     6961 caagatcggc acgagcgacg actcgctctt caagaaagac gacatgcgcg gcagcgtcta
     7021 cctcggtgca ggcgccaaat accgcgccgg cggcggctgg ggtgttcgcg tcgatgtgcg
     7081 cggcatcttc gtgcatgaca acacgggcgc ggcgttcacg aacgacgtcg agatcctcgc
     7141 gtcgctctac cgcgagtggg gccgcaagaa gacggtcaaa gtcgaagaca agccgaagac
     7201 gggcgccgat ggcgacagcg acggcatcga ggacgcgacc gacaagtgcc cgacggaagc
     7261 cgaagacaaa gacgacttcc aggatgacga cggctgcccc gacaaggaca acgatgccga
     7321 cggcgtcgcc gacgacaccg acaagtgcaa aggcgaagcg gaagacaagg acaactacca
     7381 agacgacgac ggctgccccg agctcgacaa cgatagcgac ggcgtgcccg acgcgtctga
     7441 caagtgcgcg accgaagccg agaccaagaa cggcttcgat gacgaagacg gctgccccga
     7501 cgaggttccg cccgagctga cgaaagtcat cggcccgatc acgggcgtta gcttcaaggc
     7561 gaacagcgcg gatctcgcgc cggcgtcgaa caaggcgctc gatgcggtcg ccgcgacgct
     7621 cgccaagttc ccgacggtca aggtcgaaat cggcgcgcac tccgacgacc aagcgctcaa
     7681 ggccggcggc aagttcgccg acaacgacgc actcaccgca gcccaagccg aagcagtgaa
     7741 ggcgtacctc gtgcagaagg gcgccgccga aggccagatc acggtgaagg gctacggctc
     7801 gacgaagccg atgaatgacc cggctggcct caagggcgcg aagctcgcgg ctgctcgcaa
     7861 gatcaaccgc cgcgtcgagc tgaccctcgt cgtccccgag ccggcttcca cgccgacgga
     7921 gacgaagccc gtcgagacgc cgacggcgcc gccggccgaa ggcggagcga agaccgaaga
     7981 gccgaagctc ccgtaactcg cctctccctc gaccaccgaa gcccgtggaa gcgggcggaa
     8041 ggtcgcgagg cttcgtgatg gcggccacgc gtggcgcgcg cgccggatcc aaacgactgt
     8101 gctcgtgcac gtgagtcgtc cacgtccacg cccacgtcca cgcccacgtc cacgtccacg
     8161 tccacgtcca cgtccacgtc cacgtgctcg tgctcgtgct cgtgctcgtg ctcgtgctcg
     8221 tgctcgtgtg ctcgtgcacg tgagtcgtac acgtccacgt ccacgtccac gtccacgtcc
     8281 acgtccacgt ccacgtgctc gtgctcgt
//
LOCUS       DATLBZ010000070        14037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311 k141_56541,
            whole genome shotgun sequence.
ACCESSION   DATLBZ010000070 DATLBZ010000000
VERSION     DATLBZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14037
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56541"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VMZ53_03380"
     CDS             <1..733
                     /locus_tag="VMZ53_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS27517.1"
                     /translation="RQNAAIALGTLRAAEGFEALETALREGPADLRFQAATSLAEIDQ
                     QRAFDPLLAALEDRDGQVVGAAALSVGAIAKEDAAKKQRALAALLEKLEHSNSNTRFD
                     VAYALAELGNDSGRDVLAKYFADEDRAWDAVTALVEVSAKDELAKAAASKKTQPEARA
                     LAAGKLLALDASQEFARSVLIEALGHRKPNVRGLAIEQLASIGGSWAKPPLEKLARSG
                     KGEDFLEAIAGAMKAIEARRDASAQ"
     gene            775..1608
                     /locus_tag="VMZ53_03385"
     CDS             775..1608
                     /locus_tag="VMZ53_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HUS27518.1"
                     /translation="MTPELFDSHAHVDGPEFDADRDAVLARAREAGVRRIVVIGAVND
                     PSSAERAVALAERDPNIWATVATHPHDVEKMTPEWWATFERLAPHPRVVAIGETGLDY
                     YYDHSPRQQQQDAFRRFLELAHRVNKPVVCHIRSVKEDSSRTRVEGEQDAHEDARQIL
                     REGNAAELGCVIHCFTGTPDDAKAYAEMGCYVSFSGIVTYKTAQGLRDAVSQVPRDRL
                     MIETDCPYLAPIPMRGKRNEPAFVSHTAKVVAGCAGLSFEELARITTENACRVYRLSS
                     D"
     gene            1667..2404
                     /gene="rplC"
                     /locus_tag="VMZ53_03390"
     CDS             1667..2404
                     /gene="rplC"
                     /locus_tag="VMZ53_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HUS27519.1"
                     /translation="MALNIGLLGKKIGMTQVFADDGESVPVTVIKTGPCLVLGTRTQE
                     RDGYSAIVLGFDDKPAKAATKAELGAVKATGGKPQRFVREIRLSAEEVAKFQVGQAVT
                     PSDVFKDGMPVDVEGWSKGKGYQGVIKKHHMSGMTRAHGTHEYFRHGGSIGCRLTPQR
                     VHKGKRMAGQMGNERKTVQNLQLFRILAEENCILVRGAVPGAKNEYVVVTLASTRKVY
                     KRKGMGKEEVRSKNPLKASKKAAAGRG"
     gene            2462..3139
                     /locus_tag="VMZ53_03395"
     CDS             2462..3139
                     /locus_tag="VMZ53_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HUS27520.1"
                     /translation="MSKRLDDRSTRHDRFHQKAKKEGFLARAVFKLEELDDQFKLFGR
                     GARVEGSRRSESAPGLRVLDLGCAPGSWLQFVRSRAGERATLVGLDRAPLRGDVAGAR
                     IVVGDVMTIDVKELLGDLDAFDVVLSDMAPDTSGIRSMDQARSEALFERAFEIALLTL
                     APGGHFVGKLFQGPEFKKLVEQVRSKFEQAKTAKPESSRQISIEQYVVGLRRKAEPKT
                     AAKETTP"
     gene            3136..3813
                     /locus_tag="VMZ53_03400"
     CDS             3136..3813
                     /locus_tag="VMZ53_03400"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HUS27521.1"
                     /translation="MTVRLQIEQWGDPNPRKIQQAVEILHKGGVAAYPTDSIYALGCA
                     IDARDAIEKIFRAKRMNKNQRLALICPDLSTASEFGIFNQTGFKLAQRIFPGPYTLVV
                     PATRATPRTLTDHKRRTVGIRITSHPITQALCQGLGRPLLTTSAIDPETHEVCRDADD
                     IINSFEAFVDVILDAEQTPAEPSTVLEVEDDEVKLLRMGQGPIDEFMDLIEIPPDEQP
                     RKAAGRR"
     gene            complement(3880..4350)
                     /locus_tag="VMZ53_03405"
     CDS             complement(3880..4350)
                     /locus_tag="VMZ53_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HUS27522.1"
                     /translation="MSAMSSWPMSAMSSRAASVVVSIAMLFGCGACSKDKPAGLPPPP
                     PRGSAAVTVKQPKAPPKPAELQHVTIKALGMYCEESCPLKVRYALADNPSVYELGFDT
                     ANESIFVSYDKTLGTPKDVTKPMLKAIKDAGFDPWLAKETWPADAQANVQVVLR"
     gene            complement(4371..7145)
                     /locus_tag="VMZ53_03410"
     CDS             complement(4371..7145)
                     /locus_tag="VMZ53_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS27523.1"
                     /translation="MVDSDRRPSAQEIEELIDLVRRDPASPAFIDLAEAYLNLGRPKD
                     AISVGKVGLDAAPDSLEGRVVLARAYAAMHQWKEAQGELLRVVKVDRSNRLGFALLGE
                     VLLRRNDYERAVPVLQHAQNLDPTSPQILSMLKRARMNQPLDAPTPVPQPVPPRGETN
                     YGLSLDESARQPVPAPPPSARSVPAAGPAMPTMAIQPAAADMRGDATEHQPQPYGAWN
                     EPPRQKKLTAPPPMSVEGVRPRVVSAARPQNAAAASLRQSAAVGENYLNDLLTGGLLD
                     VAGVRVPDADFDLRPDRRWGRSTRRAFIFLFVVLVVAIGGGGTYYYWTEKQKSEAITR
                     LHKEAKDSIAGASFDDLEGCLKKLGEALEKDKANALTFTYIAECGGLESLLYGTDDNR
                     VTQAIKAVQKDVKANDPGARELVIGKAAVTLATLARGDAPASALADVRKMLDDYLAKH
                     DGDKWARWLKARAMLAAGERKEAIAVLKVAADGDDGIVPAMIDQADLLVDDGKLDEAL
                     AVYKRALEKSKDHPLAVLGKAMALAESTVDSNAAIDDLSVKLDKNIGPRVGAYRSLAF
                     ALAKQNIEDYIKAGEDLKNAESKPPAPEPRFWARVAWTHYIRGEYKQAADARGRIAWF
                     GKNNAEDDPEVMLVDAALTLASGLPEKTLSIASKIEGVRPRLLRVQAQLDLKNAKGAM
                     EDLNWILERAADNLEAQMLLEVAKMMLGTDKERAASADKLESLARKTKSKIGRHMLGM
                     GYHLSGNDKDAQTQLQQAITEVTEDEPNPFLYRTRTALAEIALAAGDIKVAGDQLDEA
                     LKTNSGYLPTRIMQARVVLRNNDPDRALNLLTAVMSETQSPEVSLLQVEAICASKATT
                     AKQKDQAKDVVGKLKEFNLPPEELSRVAAVCDPKLPEQLGLPAPGAAPAPDKPAKPAK
                     PAKPNRRRGR"
     gene            complement(7167..7559)
                     /locus_tag="VMZ53_03415"
     CDS             complement(7167..7559)
                     /locus_tag="VMZ53_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27524.1"
                     /translation="MQIRELLIGALLIAACGAPPLAREEHPAPPPPSPSEPATPTEQA
                     PPTPTMGGSGAQPDGAPCLAESECASGVCEGQGCGPDQPGACAPKARGCTRDRRQYCG
                     CDGITFFSSGSCPGHRYASKGACSGSAP"
     gene            7583..8659
                     /gene="aroC"
                     /locus_tag="VMZ53_03420"
     CDS             7583..8659
                     /gene="aroC"
                     /locus_tag="VMZ53_03420"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HUS27525.1"
                     /translation="MSSSLGVLFRITTFGESHGPGLGVVVDGCPSQMPLDIAQIQAEL
                     DRRRPGQSRLVSQRKEADQVEILSGVLDGVTLGTPIAMMIRNQDARSKDYEGIAQAYR
                     PSHADYTYDAKYGIRAIAGGGRASARETAGRVAAGAIAQQLLAHRGVSIVAWVDEVAG
                     ITASVDADAVTRGAVDVNDIRCPDAARAAEMIERVERARKDGDSVGGCIRVVARGVPP
                     GWGEPVFDKLEADLAKAMMSIPAVKGVENGSGFAGTLLTGSEHNDLFYRKDDGSIGTR
                     TNRSGGIQGGISNGEAITMRIAFKPTATIMRAQETVDREGNPTTLEPKGRHDPCVLPR
                     AVPIVEAMMALVLADHSLRQAARR"
     gene            complement(8656..8955)
                     /locus_tag="VMZ53_03425"
     CDS             complement(8656..8955)
                     /locus_tag="VMZ53_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUS27526.1"
                     /translation="MGPIHAQILDELGYDPDDGRPQLHELAFVPVGRVVAARDGARAV
                     AVARLESGALVVVDDECPHDGGKISDGYVEGELLVCARHNWELRVCNGRCERVKG"
     gene            9060..10025
                     /locus_tag="VMZ53_03430"
     CDS             9060..10025
                     /locus_tag="VMZ53_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27527.1"
                     /translation="MAAVLVHIDLDGDRPHASSLVALAAGRHVASSWGATLYAALIAR
                     DESAKASLDSTAQVVTTSQMPGLEGIETALAHGGADKIVVAMTDTPVAPLWAAVGGAW
                     QGILDHLRPRLVLFGADSPSAPELGPRTGARIGARVLMRARTLGGDDVELRDRDGGYV
                     RASDSGAAVALIGRADAVAVDEDDIDVVVLALPGGADPRVELAGTQPAELSQTAGALV
                     VLADDIAADPQALADAQRLAAQLGAQLAGGKAAASIVGAGGVVDRNTALAPDLCVAVG
                     NVALDLGGSTSYVRVGASGGKTVDAALAGLPAAGVAELATRLEEP"
     gene            10022..10675
                     /locus_tag="VMZ53_03435"
     CDS             10022..10675
                     /locus_tag="VMZ53_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27528.1"
                     /translation="MSVAVWIGAAPPHRSVRWLDGALAAAAKFDNATAIAAGDQTWLD
                     LAADRANRAGLPSVGVQTELKLDYLGWAQIVAAAMRHLKATTLLVDEVSRPERFPEVA
                     AIADQLDAAQLTRVVALAPDGSVIHASRIAGRELQTIRVRGPAVIGIRIAGPPIDEYP
                     TPSPSASMKRLDLAALGLDPLVLGHRALPPRTGSSQTKKSVDRIVEHLSVHRAARRS"
     gene            10675..12159
                     /locus_tag="VMZ53_03440"
     CDS             10675..12159
                     /locus_tag="VMZ53_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HUS27529.1"
                     /translation="MAQARSAHANATEPRVQVIAPAHLGETAIAALRARGIDAREAAR
                     HIPGDDVIAWALDAPPTAASAKELAETCAAAAESGRPVCLLAPPPTSKGGARATSERA
                     AALAYLRAHGAVLVHDVDIWLEAVVALVRFGMPRGPQTAIIAPAGSWLEAQALSLVAE
                     AEAAGTRPPIVAGAAGRTSKEEPTDVVLYDPALGAPSANLPGLHIPLIARPELSDGDT
                     ALYSARAACGAIDVLGRAAERIAVGLGPAAAKDSATLELNVELRERQLGKLSAGMRVG
                     DHETKTMLSAYGVAITRQGVATTPSAAVKKARAVGYPVELKPWGHDVPTEPAGCPIER
                     GVTSDALVRRAYSAVLAASGKLPAGAQSYDDDPAAATTAMQTGAVIVREPPPLGRDLS
                     VSFVKLSSLGWTVIVDAPGAPQIAAAPAPLRVIDAENLASTIVASRAGDNEPDRRGLA
                     NLLRRASHLVVDLDDKLARLELPRVVVGGRGARTVVADAWCQLL"
     gene            complement(12166..>14037)
                     /locus_tag="VMZ53_03445"
     CDS             complement(12166..>14037)
                     /locus_tag="VMZ53_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27530.1"
                     /translation="EAVSIACAVCDGLATAHAAQVIHRDIKPDNILIASDGRVVLADF
                     GVAAVGVEGNGELSGTPAYMAPEQARGEQATPSSDVYAVAVVLFELVTGRRAFSGEMM
                     TVLDAKQAVERVMPTDGEVPAELAQVIGRATAREPAQRIATAAALRAALQPWAKPGLR
                     TTQPHRVPSLGAAGELTTIVVIAPDATVEGDGLYLAEAVHEELLARLSHLPRVRVLPR
                     PQLIPDENVFGVCMDVVGMTLAVRVTHPTGAAVSLELPLAIERIQPDAEAIASVVGAA
                     VRGQPKAASSQNIEAEELMLKARHLIQLDASKVPIAIELLDRAYAIAPDDPQVIANVV
                     IARGRFAFFRPVSADDANAYAELARANVARHPELAIAQIAAGQTELGVGDPIKAARHF
                     RAAIKCAPYFAEAHEQLGRMLLEAGYVDAALARLDDAIAIAPKLRSARWEIARARALE
                     GNWDEYERIVAQLLAEQVDRPLSRARYAWWRQDWKQLAELRTLITAADRSLWPGLFEA
                     LMAAFLDGRWLERRAEILSAIRQPTASRRRICFISQLVAEAAVVAKDHEAAIDVITWA
                     VDQGLFDIYWMDRAELLAPLRAHPRFAVLRDLVKTRADAIHDALYGDYVALSETQIA"
BASE COUNT         2310 a         4780 c         4666 g         2281 t
ORIGIN      
        1 tcggcagaac gccgccatcg cgctcggcac gctgcgtgcg gcggagggct tcgaagcgct
       61 cgagacggcg ctgcgcgaag ggcccgcgga tctgcgcttt caggcagcga cgtcgctcgc
      121 cgaaatcgat cagcagcgcg cctttgatcc gttgttggcg gcactcgagg atcgggatgg
      181 acaggtcgtt ggcgcggccg cgctttcggt gggagcgatc gcgaaagagg atgccgcgaa
      241 gaaacagcgc gcgttggccg cgctactcga gaagctcgag cattcgaatt cgaacacgcg
      301 ctttgatgtc gcatacgcgt tggccgagct cggcaatgat tcaggccgcg acgtgctcgc
      361 gaagtatttc gccgacgagg atcgcgcgtg ggatgccgtg acggcactcg tcgaggtcag
      421 cgcgaaggat gagcttgcga aggcggctgc cagcaagaag acgcaacccg aagcgcgagc
      481 gttggcggcc gggaagctgc tcgcgctcga tgcttcgcag gagtttgcgc ggtcggtgct
      541 catcgaggcg ctcggtcacc gcaagccgaa cgtgcgcggc ctcgcgatcg agcagctcgc
      601 cagcatcggc ggctcgtggg cgaagccacc gctcgagaag ctcgcgcgct cgggcaaagg
      661 cgaagacttc ctagaggcga tcgcaggtgc gatgaaagcg atcgaagcgc ggcgcgatgc
      721 gagcgcgcaa taacgcgaac gagcacgacg aacgacgaac gacggagcag tgacatgacg
      781 cccgagcttt tcgattccca tgcgcacgtc gacggccccg agttcgacgc cgatcgcgac
      841 gccgtgttgg cgcgtgcgcg tgaagcaggc gtgcgccgca tcgtcgtgat cggcgctgtg
      901 aatgatccgt cgagcgcaga gcgtgcggtg gcgttggccg agcgcgatcc gaacatctgg
      961 gcgaccgtcg ccacgcaccc gcacgacgtc gagaagatga cgcccgagtg gtgggccacg
     1021 ttcgagcgtc tcgcgccgca tccgcgcgtt gtcgcgatcg gcgagaccgg cctcgactac
     1081 tactacgatc attcgccgcg ccagcagcag caggacgcgt tccgccgatt tctcgagctc
     1141 gcgcaccgcg tgaacaagcc ggtcgtctgt cacattcgat ccgtcaaaga ggactcgagc
     1201 cgcacgcgcg tcgaaggcga acaggacgcg cacgaagacg cccggcagat tctgcgtgaa
     1261 gggaacgccg cggagctcgg ctgcgtcatc cactgcttca ccggcacgcc ggacgacgcg
     1321 aaggcctatg ccgagatggg ttgttacgtc tcgttcagcg gcatcgtcac ctacaagacg
     1381 gcgcagggcc tgcgcgacgc ggtctcccag gttccccgag accggctgat gatcgagacc
     1441 gattgcccct acctcgcccc gatccccatg cgaggaaagc ggaacgaacc ggccttcgtc
     1501 agccacaccg cgaaggtcgt cgcgggctgc gcgggcttat ctttcgagga gttagctcga
     1561 attacgaccg agaacgcttg tcgggtctac cggctatcga gcgattgact ctcaagcact
     1621 tggccggtat agattcgctc ctcgtttcta aagagagaag tctgccatgg cgctcaatat
     1681 cggcctgctc ggcaagaaga tcggaatgac ccaagtgttc gcggacgacg gtgaatccgt
     1741 ccccgtgacc gtgatcaaga cgggcccgtg cctcgtcctt ggaacacgca cgcaagagcg
     1801 tgatggctac tcggcaatcg tgctcggctt cgatgacaag ccggcgaagg ccgcgaccaa
     1861 ggccgagctc ggcgccgtga aggcgacggg cggcaagccg cagcgcttcg ttcgcgagat
     1921 tcgcctctcg gcggaggaag tcgcgaagtt ccaggttggc caagcggtca ccccgtcgga
     1981 cgtcttcaag gacggcatgc ccgtcgacgt cgagggttgg agcaagggca agggctacca
     2041 gggcgtcatc aagaagcacc acatgagcgg catgacgcgc gcccacggta cgcacgagta
     2101 cttccgccac ggcggatcga tcggctgccg cctcaccccg cagcgcgtcc acaagggcaa
     2161 gcgcatggcg ggccagatgg gcaacgagcg caagacggtc cagaacctcc agctcttccg
     2221 catcctcgct gaagagaact gcatcctcgt tcgtggcgcg gttccgggcg cgaagaacga
     2281 atacgtcgtc gtgacgctcg cgtcgacccg caaggtttac aagcgcaagg gcatgggcaa
     2341 ggaagaggtt cgctcgaaga acccgctcaa ggcctcgaag aaggcggcag cgggtcgcgg
     2401 ataagcatcc gctccgttcc gagctcaacc gcccggcgct cccgcgtcgg gcttttgttt
     2461 tatgtccaag cgtctcgacg atcgctctac ccgccacgat cgctttcacc agaaagcgaa
     2521 gaaggagggc tttctcgcgc gcgctgtctt caagctcgaa gagctcgacg atcagttcaa
     2581 gctgttcggt cgcggtgcgc gcgtcgaagg cagccggcga agcgagtcgg cgcctggatt
     2641 gcgcgtgctc gatctcggct gcgcgccggg ctcgtggctg cagttcgtgc gctcgcgcgc
     2701 cggtgagcgc gcaacgctgg tcgggctcga ccgagctcct ctccgaggag acgttgcagg
     2761 tgcgcgaatt gtcgtgggtg acgtgatgac gatcgacgtc aaggagctgc ttggcgacct
     2821 tgatgcattt gacgtcgtgt tgtcagacat ggcgccggac accagcggaa ttcgcagcat
     2881 ggaccaagct cgctccgaag ctctcttcga gcgcgcgttc gagattgcgc tcctgacgct
     2941 cgcgccgggt ggccacttcg tcggcaagct gttccagggc ccggagttca agaagctcgt
     3001 cgagcaagtg cgctcgaagt tcgagcaggc gaagaccgcg aagcctgaat cgagccggca
     3061 gatttcgatc gagcagtacg tcgtcgggct gcgccgcaaa gcggagccga agaccgcagc
     3121 gaaggagacc acgccatgac cgtgcgtctc cagatcgagc agtggggcga tccgaacccg
     3181 cgcaagatcc agcaagcggt cgagatcctc cacaagggag gcgtcgccgc ctacccgacc
     3241 gattcgatct acgcgcttgg ctgcgcgatc gacgcgcgcg acgcgatcga gaagatcttt
     3301 cgcgcgaagc gcatgaacaa gaaccagcgc ctcgcgctga tctgccccga cctgtcgact
     3361 gcgtccgagt tcggcatctt caaccagacc gggttcaagc tcgcgcagag gatctttccg
     3421 ggcccctaca cgctcgtcgt ccccgccacg cgcgccacgc cgcgcacgct gacggaccac
     3481 aagcggcgca ccgtcggcat ccgcatcacg agccatccga tcacgcaggc gctctgtcag
     3541 gggcttggtc gcccgctcct caccacgtcc gcgatcgatc cggagaccca cgaggtctgc
     3601 cgcgacgccg acgacatcat caattcgttc gaagcattcg tcgacgtcat cctcgatgcg
     3661 gagcagacgc ccgccgagcc gtcgacggtg ctcgaagtcg aggacgatga agtgaagttg
     3721 ctccgaatgg gccaggggcc gatcgacgag ttcatggatc tcatcgagat cccgccggac
     3781 gagcaaccgc gcaaagcggc cggtcgccgc taacgacgag acgccggtcg tcgctaacgg
     3841 cgcgaccccg tcgctcgagc gaagaacgac tagcctagtt tagcgaagga ccacctgaac
     3901 gttcgcttgc gcgtccgcag gccacgtctc tttcgcgagc cacggatcga agcccgcgtc
     3961 tttgatcgcc ttcagcatcg gcttcgtaac gtccttcggc gtgccgagtg tcttgtcgta
     4021 cgacacgaag atcgactcgt tcgcggtgtc gaagcccagc tcgtagacgc tcgggttatc
     4081 ggcgagcgcg tagcgaactt tcagcgggca cgactcttcg cagtacatgc cgagcgcctt
     4141 gatcgtcacg tgctgcagct cggccggctt gggcggcgcc ttcggctgct tcaccgtgac
     4201 agcggccgaa ccacgcggtg gtggcggcgg aagccctgcc ggcttgtcct tgctgcatgc
     4261 accgcacccg aacagcatcg cgatcgaaac gacaaccgat gccgcccgcg acgacatcgc
     4321 gctcatcggc cacgacgaca tggcgctcat cggtctcgac gacatcgcgc tcatcggccg
     4381 cgacgacggt tcggcttggc gggcttggcg ggcttcgccg gcttgtcagg cgcgggcgct
     4441 gcgccaggtg caggcaagcc gagctgctcg ggcagcttcg ggtcgcacac cgcggcgacg
     4501 cgcgagagct cttcgggcgg caggttgaat tccttcagct tgccgacgac gtccttcgct
     4561 tgatccttct gcttcgcggt cgtggccttg ctcgcgcaga tcgcctcgac ttgtagcagg
     4621 ctcacttcgg gcgattgcgt ctcgctcatc acggccgtca gtaggttcaa cgcgcggtcg
     4681 gggtcgttat tccgcagcac cacgcgcgcc tgcatgatgc gcgtcggcag gtagccggag
     4741 ttggtcttca gcgcttcgtc gagctggtca ccggcgacct tgatatcgcc cgcggcgagc
     4801 gcgatttcgg cgagggcggt acgcgtgcgg tagaggaacg ggttcggctc gtcctcggtg
     4861 acctcggtga tcgcctgttg cagctgcgtc tgcgcgtcct tgtcgttccc ggacaggtgg
     4921 tagcccatgc cgagcatgtg gcggccgatc ttcgacttgg tcttgcgtgc gagcgactcg
     4981 agcttgtcag ccgacgccgc gcgttccttg tcggtgccga gcatcatctt ggcgacttcc
     5041 agcagcatct gcgcttcgag gttgtcggcg gctctctcga ggatccagtt gagatcctcc
     5101 atcgcgccct ttgcattctt caggtcgagc tgcgcttgta cgcggagcag ccgcgggcga
     5161 acgccttcga tcttcgacgc gatcgacagc gtcttctcgg gcaggcctga tgcgagcgtg
     5221 agagccgcgt cgacgagcat gacttccgga tcgtcttcgg cgttgttctt gccgaaccac
     5281 gcgatgcggc cgcgggcatc ggccgcttgc ttgtactcac cgcgaatgta gtgcgtccac
     5341 gcaacgcgag cccagaagcg cggctcgggc gcgggcggct tcgactcggc gttcttcaga
     5401 tcttcgccgg ccttgatgta gtcctcgatg ttttgcttcg cgagcgcgaa cgcgaggctg
     5461 cgatacgcgc cgacgcgcgg gccgatgttc ttgtcgagct tgacgctgag atcatcgatc
     5521 gcggcgttgg aatcgacggt cgactcggcg agcgccatcg ccttgccgag caccgcgagc
     5581 gggtgatcct tcgacttctc gagcgcgcgc ttgtagacgg cgagtgcttc gtcgagcttg
     5641 ccatcgtcga cgagcaggtc cgcctgatcg atcatcgcgg gcacgatgcc gtcatcgccg
     5701 tcggcggcga ccttgagcac cgcaatcgct tctttacgct cgccggccgc gagcatcgcg
     5761 cgcgccttga gccaacgggc ccacttatcg ccgtcgtgct tcgcgagata gtcgtcgagc
     5821 atcttgcgaa catcggcgag cgcagatgcc ggcgcgtcac cgcgtgcgag cgtcgcgaga
     5881 gtgactgcgg ccttgccgat gacgagctcg cgcgcgccgg ggtcgttcgc cttgacgtcc
     5941 ttctggacgg ccttgatcgc ctgcgtgacg cgattgtcgt cggtgccgta gagcagcgac
     6001 tcgagaccgc cgcactcggc gatatacgtg aacgtcagcg cgttcgcctt gtccttctcg
     6061 agcgcctcgc cgagcttctt gaggcagccc tcgagatcgt cgaacgacgc accggcgatc
     6121 gagtctttcg cttccttgtg caggcgcgtg atcgcttcgg acttctgctt ctcggtccag
     6181 tagtagtacg tgccgccgcc gccgatcgcg accacgagca cgacgaacaa gaagatgaac
     6241 gcgcgccgcg tcgagcgacc ccaacggcga tcaggacgca gatcgaagtc agcgtccgga
     6301 acgcggacac ccgcgacatc gagcagaccg cccgtgagca gatcgttcag atagttctca
     6361 ccgaccgcgg ccgactgccg cagcgatgcc gccgccgcgt tctgcgggcg cgcagccgac
     6421 acgacgcgcg ggcggacgcc ctcgacgctc atcggcggcg gagccgtcag cttcttctgg
     6481 cgaggtggct cgttccacgc gccatacggt tgcggctggt gctccgtcgc gtcgccgcgc
     6541 atgtcggcag cggccggctg gatcgccatc gtcggcatcg cgggacccgc tgcgggaacc
     6601 gagcgagcgg atggcggcgg cgccggaacg ggctggcgcg cggactcgtc gagcgagagc
     6661 ccgtagttcg tctcgccacg gggcggaacg ggctgcggga ccggcgtcgg tgcatcgagc
     6721 ggctgattca tgcgcgctcg cttgagcatc gacaagatct gcgggctcgt cggatcgaga
     6781 ttctgcgcgt gctgcagcac cggcacggcg cgctcgtagt cattgcggcg aagcagcacc
     6841 tcgccgagga gcgcgaagcc gagccgattg ctacggtcga ccttcacgac ccgaagtagc
     6901 tcgccctgcg cttccttcca ttgatgcatc gccgcgtacg cgcgagcgag cacgacgcga
     6961 ccttcgaggc tgtccggcgc cgcgtcgaga ccgaccttgc ccacggaaat cgcatccttg
     7021 ggccggccga gatttagata cgcctcggcg agatcgatga acgccggcga tgctggatcg
     7081 cggcgaacca gatcgatgag ctcctcgatc tcttgagccg aaggccgccg atcagagtcg
     7141 accacgctcg tagagactat cacagctcag ggagcggatc ccgagcaggc acccttactt
     7201 gcataacggt gccccgggca cgaacccgac gagaagaacg tgatcccatc gcagccgcag
     7261 tattggcggc gatcgcgcgt gcagcctcgc gcctttgggg cacaggcgcc gggctggtcg
     7321 gggccgcagc cctgcccttc gcacacaccc gatgcgcact cggattccgc cagacatggc
     7381 gcgccatcag gctgcgctcc gctgccaccc atcgttggcg tcggcggtgc ttgctcggtt
     7441 ggcgtcgcgg gctcgctcgg gctcggcggc ggtggcgctg gatgctcttc gcgggcaagc
     7501 ggcggagctc cacaagccgc gatcagaagc gcgccgatca gcaattcccg gatctgcatg
     7561 cgggcatcgt acactcaccg ccgtgagtag cagcctgggc gtgctgttcc ggatcacgac
     7621 gttcggcgag tcgcacggac cggggctcgg cgtcgtcgtc gatggttgtc catcgcagat
     7681 gccgctcgac atcgcgcaga tccaagccga gctcgatcgc cgtcgccccg ggcagagccg
     7741 cctcgtcagc caacgcaagg aagccgatca agtcgagatc ctctcgggcg tgctcgacgg
     7801 cgtcacgctc ggaacgccga tcgcgatgat gatccgcaac caagacgcgc gcagcaaaga
     7861 ctacgaaggc atcgcgcagg cgtaccgccc gagccacgcc gactacacgt atgacgcgaa
     7921 gtacggaatc cgcgcgatcg ccggtggggg tcgcgcgagt gcacgcgaga ccgcaggacg
     7981 cgtcgccgcc ggagcgatcg cgcaacagct gctcgcgcat cgcggcgtgt cgatcgtcgc
     8041 gtgggtcgac gaggtcgcgg gcatcaccgc gagcgtcgat gcagatgcgg tcacccgcgg
     8101 cgctgtcgac gtaaacgaca tccgctgtcc ggacgccgcg cgcgcggccg agatgatcga
     8161 gcgcgtcgag cgcgcacgca aagacggtga ctccgtcggt ggctgcatcc gcgtcgtcgc
     8221 acgtggcgtg ccgcccggct ggggcgagcc ggtattcgac aagctcgaag ctgacctcgc
     8281 gaaggcgatg atgagcatcc cggcggtcaa aggcgtcgag aacggctcgg gcttcgcggg
     8341 cacgctactc acgggcagcg agcacaacga cctcttctat cggaaggacg acggatcgat
     8401 cggcacgcgc acgaaccgct cgggcggcat tcaaggcggc atctcgaacg gcgaagcgat
     8461 cacgatgcgc atcgcgttca agccgaccgc gacgatcatg cgcgcgcaag agaccgtcga
     8521 tcgcgaaggc aatccgacga cgctcgagcc gaaaggccgt cacgatccgt gcgtgctccc
     8581 gcgcgccgtg ccgatcgtcg aagcgatgat ggcgctcgtg cttgccgatc actcgctgcg
     8641 gcaagccgcg cgtcgttagc ccttcacgcg ctcgcagcgg ccgttgcaga cgcgcagctc
     8701 ccagttgtgg cgcgcgcaca cgagcagctc gccctcgacg tagccatcgc tgatcttgcc
     8761 gccgtcgtgc gggcactcgt cgtcgacgac gaccagcgcg ccactctcga gccgagccac
     8821 tgcgaccgcg cgcgcaccat cgcgggccgc gacgacgcga ccgaccggta cgaacgcgag
     8881 ctcgtgcagc tgcgggcggc cgtcgtccgg gtcgtagccc agctcgtcga ggatctgcgc
     8941 gtggatgggg cccacagcgg gtcacgctac ggctcccctc tgacgctgca gcgcacccgc
     9001 cgggccaagt ccgcgcaatt cttgccatcg tcggagatct cacttacctt cgagcgaccg
     9061 tggcggctgt cctggtccac atcgatctcg acggcgaccg cccgcacgct tcgtcgctcg
     9121 tggcgctcgc ggcgggacgc catgtcgctt cgtcgtgggg cgcgacgctg tacgcggcgt
     9181 tgatcgcgcg tgacgagagc gcaaaggcct cgctcgattc gacggcgcag gtcgtgacga
     9241 cgtcgcagat gcccgggctc gaaggcatcg agaccgcgct cgcgcacggc ggtgccgaca
     9301 agattgtcgt cgcgatgacc gatacgccgg tcgcgccgct gtgggctgcg gtcggtggtg
     9361 cgtggcaagg cattctcgat cacctgcgcc ctcgcctcgt gctgtttggt gcggacagcc
     9421 cgtcggcgcc ggagctcggg ccgcggaccg gtgctcgcat cggcgcgcgt gtactgatgc
     9481 gcgcacgcac gctcggtggt gacgatgtcg agctgcgcga tcgcgatggc ggctacgtgc
     9541 gcgccagcga tagcggcgcc gcggtcgcgt tgatcggtcg cgcagatgca gtcgccgtcg
     9601 acgaggatga tatcgacgtg gtcgtgttag cgctgccggg cggcgcggat ccgcgcgtgg
     9661 agcttgccgg cacacagcct gccgagctct cgcagacggc tggcgcgctc gtcgtgctcg
     9721 ccgatgacat cgccgcagat ccgcaggcgc ttgccgatgc gcagcgactc gcggcgcagc
     9781 tcggcgcgca gctcgccggt ggtaaggcag ccgcgagcat cgtgggtgca ggcggtgtcg
     9841 tcgatcgcaa tacggcgctc gccccggatc tgtgcgtcgc cgtcggcaat gtggcgctcg
     9901 atctcggtgg ctcgacgagc tacgtccgcg tcggcgcgag tggcggcaag accgtcgacg
     9961 ccgcgctcgc aggccttccc gcagcgggcg ttgccgagct cgcgacgcgg ctggaggaac
    10021 cgtgagtgta gcggtgtgga tcggcgccgc accgccgcat cgctccgtgc gctggctcga
    10081 cggcgcgctc gctgccgcgg cgaagttcga caatgccacc gcgatcgcag cgggcgatca
    10141 gacgtggctc gatctcgcgg cggatcgcgc gaatcgcgcg ggcttgccgt cggtcggagt
    10201 gcagaccgag ctcaagctcg attatctcgg gtgggcgcag atcgtcgctg cggcgatgag
    10261 gcacctgaaa gcaacgacgc tgctcgtcga cgaagtgagc cggcccgaac gctttcccga
    10321 ggtcgccgcg atcgccgacc agctcgatgc cgcacagctc acgcgcgtcg tcgcgctcgc
    10381 gcccgatggt tcggtcattc acgcgagtcg catcgccggt cgcgagctgc aaacgatccg
    10441 cgtccgcggc ccggcggtga tcggcattcg catcgcaggc ccgccgatcg acgagtaccc
    10501 aacgccgtcg ccgagcgcgt cgatgaagcg cctcgatctg gcggctctcg gcctcgatcc
    10561 tctcgtgctc ggccaccgcg cgttgccgcc acggacggga agctcgcaga cgaagaagag
    10621 cgtcgatcgc atcgtcgagc acctgtccgt tcaccgcgct gcacggagat cctgatggcg
    10681 caggcgcgct cggcacacgc gaacgcgacg gagccgcgcg tgcaagtgat cgcgccggca
    10741 cacctcggcg agacggcgat cgccgcgcta cgcgcacgcg gcatcgatgc tcgcgaagct
    10801 gcgcgccaca ttcccggcga tgacgtgatc gcgtgggcgc tcgacgcgcc gccgactgca
    10861 gcatcggcga aagagctcgc cgagacgtgt gcggccgccg ctgaatccgg tcgccccgtg
    10921 tgccttctcg caccaccgcc gacgagcaaa ggcggcgcgc gcgccaccag cgagcgggcg
    10981 gcggctctcg cctatctgcg cgcacacggc gccgtgctcg ttcacgatgt cgacatctgg
    11041 ctcgaagcgg ttgtcgcgct cgtccggttc ggaatgccgc gcggaccgca gaccgcgatc
    11101 atcgcgccgg ctggctcgtg gctcgaagcg caagcgctct cgctcgtcgc cgaggctgaa
    11161 gctgcgggta cgcgaccgcc aatcgttgca ggcgccgccg gtagaacctc gaaggaggag
    11221 ccaacggatg tcgtgctcta cgatccagcg ctcggggcgc catccgcgaa tctccccggc
    11281 ttgcatatcc cgctgatcgc caggcccgag ctctccgacg gagacaccgc actctacagc
    11341 gctcgcgctg catgcggagc gatcgacgtc ctgggtcgcg cagctgaacg catcgcggtc
    11401 gggctcggtc cggccgcggc gaaagactcc gcaacgctcg agctcaacgt cgagcttcgg
    11461 gaacgccagc tcggcaagct cagcgcaggg atgcgcgtcg gcgatcacga gacgaagacg
    11521 atgctctcgg cctacggcgt ggcgatcaca cgccaaggcg ttgccacaac gccgtccgcc
    11581 gccgtcaaga aagcgcgcgc tgtgggctat cccgtcgagc tgaagccgtg gggccacgac
    11641 gtgccgacgg agccagcggg ttgtcccatc gagcgtggcg tgacgagtga tgcgctcgtc
    11701 cgtcgcgcgt actcggcggt gctcgccgcc tccggcaagc tgccggcggg cgcgcagtcc
    11761 tacgacgacg atcccgccgc cgcgacgacg gcgatgcaga ccggcgcagt gatcgtgcgc
    11821 gagccgccgc cgctcggtcg cgatctctcc gtgtcgttcg tgaagctgtc gtcactcggc
    11881 tggacggtca tcgtcgacgc accgggcgcg ccgcagatcg ctgcagcccc tgctccgctg
    11941 cgcgtgatcg atgccgagaa cctcgcgtcg acgatcgttg cgtcgcgtgc cggtgacaac
    12001 gagcccgatc gccgtggcct cgccaatctg ctgcgccgcg catcgcacct cgtcgtcgat
    12061 ctcgatgaca agctcgcgcg cctcgagctg ccgcgcgtcg tcgtcggcgg tcgcggcgct
    12121 cgcaccgtcg tcgccgacgc gtggtgtcag ctcctctgac gcgagctacg cgatctgcgt
    12181 ctcggagagc gcgacgtaat cgccgtacag cgcgtcgtga atcgcatctg cgcgcgtctt
    12241 gaccaggtcg cggagcacgg caaaccgtgg gtgcgcacgc agtggcgcga ggagctcggc
    12301 acgatccatc cagtagatgt cgaacagtcc ctggtcgacc gcccacgtga tgacgtcgat
    12361 ggcggcctcg tgatccttgg ccacaaccgc cgcctcggca accagctgcg agatgaagca
    12421 gatccggcgt cggctcgccg tcggctgccg aatcgcggag agaatctcgg cgcgccgctc
    12481 cagccaccgc ccgtcgagga atgccgccat cagcgcttcg aagaggccgg gccacaggct
    12541 gcgatccgcc gcggtgatca gcgtgcgcag ctcggcaagc tgcttccagt cctgtcgcca
    12601 ccacgcgtag cgcgcacgtg acagcggacg atcgacttgc tccgcgagca gctgcgcgac
    12661 gatccgctcg tactcgtccc agttgccctc gagtgcgcgg gcgcgcgcaa tctcccagcg
    12721 cgcagatcgc agcttggggg cgatcgcaat tgcatcatcg aggcgcgcga gtgccgcgtc
    12781 gacatagccg gcctcgagca gcatgcgtcc gagctgctcg tgagcctcgg cgaagtacgg
    12841 cgcacacttg atcgccgcgc gaaaatgccg cgctgccttg atcggatcgc cgacgccgag
    12901 ctccgtctgc ccagccgcaa tctgtgcgat cgcgagctcg ggatggcgtg cgacgttcgc
    12961 gcgcgcgagc tccgcgtacg cattcgcatc atcggcgctt accggccgaa agaatgcaaa
    13021 gcgcccgcgc gcgatcacga cgttcgcaat gacctgaggg tcatcaggcg cgatcgcata
    13081 cgcgcgatcc agcagctcga tcgcgatcgg aaccttcgac gcgtccagct ggatcaggtg
    13141 ccgagccttc agcatcaatt cctcagcctc gatgttctga ctcgacgccg ccttcggctg
    13201 gccacgcacc gccgcgccca ccaccgacgc gatcgcttcg gcatctggct ggatccgctc
    13261 gatcgcgagc ggaagctcga gcgagacggc ggcgccggtc ggatgcgtga cgcgcaccgc
    13321 aagcgtcatc ccgacgacgt ccatacacac accgaacacg ttctcgtcgg gtatcaactg
    13381 cggccgcggc aatacgcgca cgcgcggcag atgcgacagc ctggccaaga gctcttcgtg
    13441 aacggcctcg gcgaggtaga gcccatcgcc ctcgacggtc gcatccggtg cgatcacgac
    13501 gatcgtcgtc agctcgccgg ccgcgcccag cgacggcacg cgatgcggct gcgtcgtcct
    13561 gagccccggc ttcgcccacg gctgcagcgc cgcccgcagc gccgccgcgg tcgcaatgcg
    13621 ctgcgccggc tctcgcgcgg tcgcccgccc gatcacctgc gccagctccg ccggcacttc
    13681 gccatccgtc ggcatcacgc gctcgacggc ctgcttcgcg tcgagcaccg tcatcatctc
    13741 gcccgagaac gcgcgtcgcc cggtcacgag ctcgaacaag accaccgcca ccgcatacac
    13801 gtcgctcgac ggcgtcgctt gctctccgcg cgcctgctcg ggcgccatat acgccggcgt
    13861 ccccgacagc tcgccattcc cctcgacgcc gaccgccgcc acgccaaagt ccgccaacac
    13921 gacgcgccca tcgctcgcga tcaaaatatt gtccggcttg atgtcgcgat gaatgacctg
    13981 cgcagcgtgc gccgtcgcca gcccatcaca aaccgcgcac gcgatgctca cggcctc
//
LOCUS       DATLBZ010000071        15541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5991300, whole genome shotgun sequence.
ACCESSION   DATLBZ010000071 DATLBZ010000000
VERSION     DATLBZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15541
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5991300"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VMZ53_03450"
     CDS             <1..733
                     /locus_tag="VMZ53_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27531.1"
                     /translation="MSHLSSMQIDGLASGLVVEPAARAHIDECEQCRADLAATEVAMA
                     HFKSSILPRTVTRLTPRTSWKRWLPALVVPIVAAAAVILWLGREEPGRPDESDIAIKG
                     DVTFQVFAKRGNDVLQLRDGTKLRAGDSIRFVAGAQTARYLLVGSVDGAGKPTLYYPY
                     GGPRSGAIGKVPAELPGSIVLDAAPGPERVFAIFSADPIEGDAMTRALAELGGKGADA
                     IRAAHTIVVPGAKTAVTVVFEKETP"
     gene            730..909
                     /locus_tag="VMZ53_03455"
     CDS             730..909
                     /locus_tag="VMZ53_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27532.1"
                     /translation="MRLVVLVLVALTASAHAETKRVAVVVGNNAGNGELAPLRFAEID
                     AGKVSRVLIELGGVE"
     gene            949..1341
                     /locus_tag="VMZ53_03460"
                     /pseudo
     CDS             949..1341
                     /locus_tag="VMZ53_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
     gene            complement(2046..2609)
                     /locus_tag="VMZ53_03465"
     CDS             complement(2046..2609)
                     /locus_tag="VMZ53_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27533.1"
                     /translation="MRKLIALTLLSGCGSTLFAITPRSQPAIVEVSHVEHTYGGYPVV
                     HRELHRRSTKTIVPALVGMMVDAVVTTALFASASGNGDAEAIMVAGLVGTAAVALDVH
                     LISNRAIVDERLEAVAYQPGWPQGAIEMPSQAPGSGYSTVPPSFSIQVSGSCPATPSC
                     TVPATRIVMPPPAPTPNTPVELVPAND"
     gene            complement(2646..3005)
                     /locus_tag="VMZ53_03470"
     CDS             complement(2646..3005)
                     /locus_tag="VMZ53_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS27534.1"
                     /translation="MRSLLVACLLAAACGNKPAPTRPVDESKTADAGVASSGSGSAAG
                     PAEEKIGVALQVDPTEANVTIDDLVVGRASDLDPVFALAPGLHTLVITHPGYKTYRME
                     FSVTDKTESFTIHLDKK"
     gene            complement(3025..4992)
                     /locus_tag="VMZ53_03475"
     CDS             complement(3025..4992)
                     /locus_tag="VMZ53_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HUS27535.1"
                     /translation="MRSVGSLIIVAAIAACTTPGSGPALSSIDPPSGTSSAETPVTIT
                     GSNFRLPLVADLDHGDIDVAALSATIGGVPLSSVTQTTDATIEAVVPAGLPQGVHDVV
                     VSFGTRTATLEDGYTVVSGTLVSTLSVARWLGKGNTFSVSMTITNNGTETVDAVKPSM
                     IATSGTGAVTMVGGAVASVPLPPNSIIALAFAYTADDYGTVIFSGNATGVDATASPIA
                     SAVTMATADIVETAVVATDPFTDGSTFAYATAYNDQVYLGPNDTGTRAVRMRPSDPTS
                     EKLSFTFTKDTTGNSTSNGSATYTSIGATGCVADTPACGPDNENGRGTFASGMLDGSE
                     WLVIGGARSTGDLDYVYMTNDTDTTLDFRYVDLSPVMGAATRGFSAAYALNGRFYLGF
                     PDNGGARPYFIALLASPPAPGLDTTAATAINLDADKFPGWQAAAIVNIDSMTNLSGRL
                     FVSSVAGIMAATTTTPAPYGTTPTDWYVATPSSVVFTGKASKPPTKLADIFPSDRAFP
                     QMATFNNRVYAGRNTTTGPQLWMCDPTLGILPDRCEPQDWSIAAANVAGDPALTQFED
                     ASLTAISMVVATPQSLYVGFDSANGVKVFRSSAAAPFFQSDFQAVGTAGLGAATNTRI
                     LDGRALTFSTGTSVWITAGSATSALQLVVIP"
     gene            complement(4976..7117)
                     /locus_tag="VMZ53_03480"
     CDS             complement(4976..7117)
                     /locus_tag="VMZ53_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27536.1"
                     /translation="MERKALAAAIVVASMAQLAHADDKVDVKVTDSVVGDGRSFAPVT
                     VTGGPKVQGKALPVPDVATVTCANAAGLAAPPNGLPAVLAPVATAATTLECKAQSRGA
                     ETSFKLKVTPPPAGLYAGVAAMAKSTDAKIEVPVISWDGKKASAPGALKASASDGTVD
                     VAGAALTLSVSGKVPRLVAIAMLDGTRAGAAFVPVIGVTTLPVESDTNSNVEVWIAGT
                     WFGPVPTKGKTAEVPIEVPPGITHAVARSTSQRGYVTDAVTDLKIPVRLRIGAVAGST
                     KLAAGEKTLIAIAVAGADGRPAAASAKVVATASKGALGAIQSKGGGLWTVEFTAPTTP
                     GNERVSIRVEGDPNAGTGEVPVEIGGGAASKLELGQPTSPVVPGGELVVPIHMTDASG
                     NVLDGSTATATLGGTNVQITDGAVHGKIPEVLPPSGTLKLVVDAGGVQQSLDLKPSSP
                     AVSATVSIDVDGRDAELVIVVRDKFGNLVQDGTFDLAVTGGAVRGVTRTERGFRARVA
                     AAEHAADADIVVRAGGQSLAQTSVHFDPPSHAFVVGAWAAGGWMDNLGALKSPRAALG
                     LGLRRGGTVELGLSAGVEGIMTSESTTVDVMGMPDKTDRSIQGLGVPVLARAHVRITR
                     RLGVTLAGGVVPMRVKVELTPTLQPADRDAATVIGFRGQLTADVRLGPGRAFAGASYG
                     RAKLEAANAVGQLDGLGLVAGYEWWFGAFGW"
     gene            complement(7099..8340)
                     /locus_tag="VMZ53_03485"
     CDS             complement(7099..8340)
                     /locus_tag="VMZ53_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27537.1"
                     /translation="MHLRVVIACVAFTGACKGHDKDKAPPAASNGSAETAKPAEPSVP
                     TLATVAELGGNVKVKRKGATEWTPAEKDEALHAGDAVRTADESFVRLKLAGRGTLEIS
                     EPTTVIIDDALSLQDGTVMAVVDPGATLVVKAADGSEAHIAAPSGASQPSTFRISPAA
                     DNSLDIAAIQGDVAVKTQAGEQAVTAGKVTRVVKQSASEPVSLIAFPKSVAPGIDARF
                     HFVADKPIDLQWATVKEASGYRVQISKDTEFRQLVLNEDATSTAVQFAPKAPGVYAWR
                     VAARDKSDHLGEYGFGRRIYFEQEPPKDLLLTPPDGAKVGFSDSYPKIEFSWQSLGDA
                     SEYKLVVGRGAVPSVDPIVTIPSSGQKVEVGSLREGVYHWGVYAVVDGHDEPIFMTAR
                     EITIRKQRVKAHTENLWNEKR"
     gene            complement(8355..10394)
                     /locus_tag="VMZ53_03490"
     CDS             complement(8355..10394)
                     /locus_tag="VMZ53_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27538.1"
                     /translation="MTTPQKRALEALGWIVLTIPALYQLLLLATAIAGRISYPYDLEW
                     MEGGMLHHALRFQQGTGIYNAPSIEFIPYLYTPLYPALLAALGGIFGLTYTLGRVVSV
                     LSLVGIAGAGIVSICAPRFRHTSWWPALAGSVLAIGLFAATYPFVEGWFDLVRADTLF
                     LFLITFGLMGALFWSPEGIGINGHGRVIALAAIFAFAFFAKQTGIVYVALGGLVVLVM
                     AWRRVGTYAITAAFIGFGGTGLMNTGSGGWFWVYVRKIHNAHDFNYDRFWKSYGNILW
                     HFRAMTIIVAITLVVVLVTWLRRPAEGIRRELPRQTRPFLLWTATFGVSTIVGAIGWG
                     TEFAHFNAYMPAFLHGALAAGAAIPALAACTGILWGPRRFPRLVSHGIAAVAAIALAV
                     TCWHWQWNPQRFIPTQRDVAAGDALIKRIAALQGDVWMPSHPWYLRLAGKQPHVHRMG
                     IKDVTWRQNRMVLGLDEALQSHRFSAIIFDNRDLFLELPQVRTYYRPALKLPANEQPH
                     LYTGALIVPESIWVPALAAAPPKGAKVVFDFEGATWQGWTKSGGAWGNGPEAESQPGQ
                     ELVIGATGQRFATSMHGGDSATGRVTSPSFALDGAKLSLKLGGGTDATKLRVELWVDG
                     QIAKSGGPPSLGGDVLRELTWDIGELRGKWGTLVLVDDATMADGHLDVDDVWLWD"
     gene            complement(10391..13036)
                     /locus_tag="VMZ53_03495"
     CDS             complement(10391..13036)
                     /locus_tag="VMZ53_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUS27539.1"
                     /translation="MASGPRSVSHVLGTGALAGLFAGLAAGLIDALWSWGPAAQFVPS
                     FVSRVRFVVYSGLALAATGALAGFIGAAILGHLSRRTRLGDLVRFGWGEHRARQEKDP
                     RDAIIGMSLVIPGVPLVALAIYVVFRMLTPVVAKSHAMGLAVLSVIAATIAALLAAII
                     AMFVVAQPVELALRKIAKRIPIIAEPTAPLVVGGVLIAAILAWLAHAQWTTAKILPLR
                     GPLVFVFGALLAIPAYRPASTACDFIGMFRPAIRRTIWVVWPIALFILVLGAGGSAPV
                     IKASTAYTGLGAPIARGVRKAFDRDRDGYSRFLGGGDCDDGDRTVHPGAPEIPDDGID
                     QNCVGGDASMKATHEDVAFAPVPATVPPGMNILLVTIDTTRADHLGMYGYKRPTSPNM
                     DALAAQGTVFDHGWAHAPSTRYSMPAILSGRLPLDVFYDYAVGGWPGLAPKATTLAEY
                     LAPLGYITGAITNFWYFSRDRHMDQGFGEYDNENYRLHGDVGGPEHSKGSSSKEQTDK
                     AIAFVDRHAAEKWFLWVHYYDPHADYEGHPEVPSFGSDPVALYDNEIRFTDLHLGRLF
                     DDLRAKGLYDKTIVIITGDHGEGFGEHRIDHHGYHLYSAQTKVPLIIRVPGLPPRHAT
                     TPAGHIDIAPTLVNLAGGQPGPDMMGRSLVDVLGGADTKRTVFQQLSFENNNEKRAAV
                     DERCHVIYNVSPETSWEVYRVDRDPLEAHDLEGGEECTETRHVLERWYDVAQVPVGAA
                     EALLPSGAGLPAPLDATIGSGARLLSVEAPARAKPGDQITLTWTFEAKGRVSNDWKIF
                     AHLKGPANAFVNGDHKPVRPFEWWKPGQFIRYTTTLVLPRTLPVGPYTLWVGFFRGNE
                     RAPVKSVAAKLDNNAVAAATIEISP"
     gene            13088..14695
                     /locus_tag="VMZ53_03500"
     CDS             13088..14695
                     /locus_tag="VMZ53_03500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUS27540.1"
                     /translation="MTTDRLRWLLVGVAAIVLVLHAFAYNFITDDAYISFVYSRNFAE
                     HGELVFNLGDYVEGYTNFLWTLLIGLGMLVHIPPEWSAKILGALFAVLTLWVTFVVVE
                     RALGRKTLWATVPPLLLACSAGFACWTSGGLETQLFTMLCTIAIEGLVGAEQEDGASG
                     LYRAAVALALAAMTRPEGMLIAAALGVVRIICNVSSKRGLLGKTELVAAICFLVLWAP
                     WFAWRWNYYGYPFPNTYYVKATGDWASPEMARQMREAGMYYVWIWLKQTKLVFALPLV
                     FIGLVGARPRTPRFALGLSCALIAAFYIPYTISVGGDFMGLHRFIMPLFVVAAIAVVL
                     GLELLCSLIPESARKYVALALGLALVGMFAGTQLQLTQKSLAWGNFNPDHGIDTPAFL
                     ITYTEDRALIGQAMQPCFKPDDFSIVGGAGAQPYYGRMQAIDVFGLVSDRVAHREKRI
                     RPRAGHTKFASDALLAEYEPTFIFSCYAIHPTPARPTTLNCNTGFWLSRGYEMVTMHV
                     PGMKQSGEYYTFLAKKDRNFECPGRVP"
     gene            14746..>15541
                     /locus_tag="VMZ53_03505"
     CDS             14746..>15541
                     /locus_tag="VMZ53_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27541.1"
                     /translation="MVRALLHTAPLDNLSPAPAEASDPPGTRAYAGYVMISRGGPVII
                     GFQSDGPARLVVGTHRLIAGRGLIKDRAVLPAGPTPIRFVAPPGARLVWNPVGRRGDP
                     EYLPASSLSRTPDGFGHFVGAAVLDGVIALLLLLTLVGSLLYLARGRLRAVDSHTWLA
                     IGGVFAGALLVRVIGLNAFGQTWDEDVNWGAGRNYITNILSGDLSEGSWIWNFEHPPV
                     MKYLEGIGAQFADGFGPARFLSAIWTSLGCALLVPIGMRLYRLRVGI"
BASE COUNT         2551 a         5142 c         5217 g         2631 t
ORIGIN      
        1 catgagccac ttgtcgagca tgcagatcga tggcctggca tcggggctcg tcgtcgaacc
       61 ggcggcgcgc gcgcacatcg atgagtgcga gcagtgtcga gcggacctcg ctgcgaccga
      121 agtggcgatg gcgcatttca agagcagcat cctcccgcgg acggtcacga ggcttacgcc
      181 gcgcacatcg tggaagcgct ggttgcctgc gctcgtcgtg ccgatcgttg cagccgccgc
      241 cgtcattctc tggctcggcc gcgaggagcc cggacgcccg gacgagtcgg acatcgcgat
      301 caagggcgac gtcacgttcc aggtgttcgc gaagcgcggc aacgacgtgc tgcagctacg
      361 cgatgggacg aagctgcgcg ccggcgattc gatccgattc gtggccggtg cacagaccgc
      421 gcgctatctc ctcgtcggtt ccgtcgacgg cgcgggcaag ccgacgctgt actatccgta
      481 cggtggtcca cgcagcggcg cgatcgggaa ggtacctgcg gagcttccgg gcagcatcgt
      541 gcttgacgca gcgccagggc ccgagcgggt cttcgcgatt ttctcggcgg atccgatcga
      601 aggtgatgcg atgacacgtg ctctcgcgga gctcggtggc aaaggggcgg acgcgatccg
      661 tgcggcgcac acgatcgtcg tgcccggagc gaagacggca gtcaccgtcg tcttcgagaa
      721 ggagacgcca tgaggctggt cgtgttggtc ctcgtcgcgc tcactgcatc cgcacacgcc
      781 gagacgaagc gcgtcgcggt cgtcgtcggc aataacgcgg gcaacggcga gctcgcgccg
      841 ctgcggttcg ccgagatcga tgcgggcaag gtcagtcgcg tgctcatcga gctcggcggc
      901 gtcgagtaag acgacctctt cctgctgcag ggcaaagacc tcgccacgct gaaggccacg
      961 ctcgatcgcg cgaaggcgaa ggtcgccgcc tatcaaaagg gcgtcgatcg cgtcgtcttg
     1021 ctcttctact actcgggcca ctccgacggt atcgcgctcg agctcggcaa cgatcgctac
     1081 acgtttgcag cgctccgcca gtggctcaaa gacacgaacg ccgaggtccg cgtcggcttc
     1141 atcgactcgt gcaagagcgg cgcgctgctc ggcacgaagg gcggctcgcc gggcccagcg
     1201 tttcagatcc gcctgaccga tgatctatcg agcaccggcg aagcgttgct cacgtcgagt
     1261 gccgccgacg agaacgcgct cgagtcgaag gaaatcggcg ggtcgttctt cacacatcat
     1321 ctcgtctcgg gactacgcgg cgcggccgac agctccggtg actcgcgcgt gacactctcg
     1381 gaggcgtacc agtacgcgta caagcacacg atcgcgacgt cgggtgcgac gatcgcaggc
     1441 ccgcaacatc cggcgtacga ctaccagctg tcagggcagg gcgagctgat cctgaccgag
     1501 ctcgcgcaac ccacggcggc gctcgcgctt cccgacggct tcgaccgggc gctcgtgatc
     1561 gaggtcgcgc gcgaccaggt gatcgcagag cttggtcctg gtgcgcctgc acagctcgcg
     1621 gtgatgcccg gcaagtatgg cgtgcgcgcg tggaagcagg acaaggtctt cgagggtcgc
     1681 gtcaacgtcg gcgcgaacgc ggtgcgcgcg gtgcgctggg acgagctctc tgcgatcacg
     1741 ctgcccgcga ttcgctcgaa gggcgacgtc gtgtactacg gcgtgccgca ggagcgacac
     1801 gacctcgcgt tcgaggcggg gttcggcgtg cggcgccaga tcgccgacga cttgcaagga
     1861 acgctgccga gcttccgcgt cggcgcgcgc tatcgccact acgcactcga catcgatttc
     1921 atgacgaacc tcagcaagct cgtgcgcgag acgatcggca tcgcgacggt cggctaccgg
     1981 ttctcgacga atacgggccg gctccacgcg tggatcggac cggagatcac ggcgggtttc
     2041 gtcactcagt cattcgcggg aacgagctcg accggcgtgt tcggcgtcgg cgccggcggc
     2101 ggcatcacga tgcgcgtggc aggcaccgtg cagctcggcg tcgccggaca actgccgctc
     2161 acttggatcg agaaagatgg cgggaccgtg ctgtacccgc tacccggtgc ctggctcggc
     2221 atctcgatgg cgccctgagg ccagcccggc tggtacgcca ccgcctcgag acgctcgtcg
     2281 acgatcgcgc gattcgagat gaggtggacg tcgagtgcga cggcagccgt cccgaccagg
     2341 cctgcgacca tgatcgcctc cgcatcgcca ttgccgcttg ccgaggcgaa cagcgcggtc
     2401 gtgacgacgg cgtccaccat cattccgacg agcgcgggta cgatcgtctt cgtactgcgt
     2461 cgatgcagct cgcgatgcac gaccggataa ccgccgtacg tgtgctcgac gtgcgagacc
     2521 tccacgatcg cgggctggga ccgaggcgtg atcgcgaaca gcgtgctgcc gcaaccggag
     2581 aggagggtga gggcgatgag cttgcgcatg caagtaccac ccggcgcgcg ccgctcgtgc
     2641 gcagattatt tcttgtcgag gtgaatcgtg aaggactcgg tcttgtcggt caccgagaac
     2701 tccatccggt acgtcttgta gccggggtgc gtgatcacga gcgtgtgcag gcccggtgcg
     2761 agcgcgaaca caggatcgag atccgacgcg cgcccgacga cgagatcatc gatcgtgacg
     2821 ttggcttccg ttggatcgac ctgcagcgca acgccgatct tctcctcggc cggccccgcg
     2881 gcgctgccgc tccccgagct cgcgacgcct gcgtcggcag tcttcgactc gtcgaccggc
     2941 cgcgtcggcg cgggcttgtt gccacacgcg gcggcgagaa ggcaggcaac gagcaacgac
     3001 ctcatccagc cattctagcg ctgtttacgg gatcacgacg agttgcagtg cactggtcgc
     3061 gctacccgcg gtgatccaca ccgatgttcc cgtcgagaac gtcagcgctc gaccgtcgag
     3121 gatccgggtg ttcgtggccg ctccgagacc cgctgttccg acggcttgga agtcgctctg
     3181 gaagaacggc gccgcggcgg agcttctaaa gaccttcacg ccgttcgcgc tgtcgaagcc
     3241 gacgtagaga ctctgcggcg tcgcgaccac catcgagatc gcggtgaggc tcgcgtcctc
     3301 gaattgcgtg agcgccgggt cacccgcgac gttggctgca gcgatcgacc agtcctgcgg
     3361 ctcgcagcga tcaggcaaga tgccgagcgt cgggtcgcac atccagagct gggggccggt
     3421 cgtcgtattg cgtccggcat agacgcgatt gttgaacgtt gccatctgtg ggaacgcgcg
     3481 atcggacggg aagatgtcgg cgagcttcgt cggaggcttc gacgctttgc cggtgaacac
     3541 gacgctgctc ggagttgcta cgtaccagtc cgtgggagtg gtgccgtacg gcgccggcgt
     3601 cgtcgtcgtc gcggccatga ttccagcgac gctcgacacg aacaggcggc cgctgaggtt
     3661 cgtcatgctg tcgatgttca cgatcgcggc ggcctgccag ccgggaaact tgtccgcatc
     3721 gaggttgatc gcggtggccg ccgtcgtgtc gagtccgggc gccggcggcg atgcgaggag
     3781 ggcgatgaag taggggcggg cgccgccgtt atcggggaag ccgaggtaga agcggccgtt
     3841 gagcgcgtac gcagccgaga agccgcgcgt ggccgcgccc atcacgggag acagatcgac
     3901 gtagcggaag tcgagcgtcg tgtcggtatc gttcgtcatg tagacgtagt cgaggtcacc
     3961 cgtcgagcga gcaccaccga tgacgagcca ctcgctgccg tcgagcattc cagacgcgaa
     4021 cgtgccgcga ccattctcgt tgtcgggccc acatgccggc gtgtccgcga cgcagcccgt
     4081 cgcgccaatc gatgtgtagg tcgccgagcc gttcgacgtc gagttacccg tcgtgtcttt
     4141 cgtgaacgtg aacgagagct tctcgctcgt tggatcactc ggccgcatgc ggacggcgcg
     4201 cgttccggtg tcattcgggc cgaggtagac ctggtcgtta tatgcagtcg cgtacgcgaa
     4261 cgtactgccg tcagtgaacg ggtcggtcgc gacgacggcg gtctcgacga tatcggcagt
     4321 cgccatcgtg acggccgacg cgatggggct cgcggtcgca tccacgccgg tcgcgttgcc
     4381 ggagaagatc acggttccgt agtcgtcggc ggtgtacgcg aacgccagcg cgatgatcga
     4441 attcggcggc aatggcaccg aggcgacagc gccaccgacc atcgtgaccg caccggtacc
     4501 gctcgtcgcg atcatcgacg gcttcacggc gtcgacggtc tcggtgccgt tgttcgtgat
     4561 cgtcatgctg acggagaacg tgttgccttt gccgagccac cgcgcgaccg agagcgtcga
     4621 gacgagcgtg ccgctgacga ccgtgtagcc atcctcgagc gtcgcagtgc gcgtgccgaa
     4681 gctgacgacg acatcgtgca cgccctgggg caggcccgca ggcacgacgg cttcgatcgt
     4741 cgcgtcggtg gtctgcgtga cgctcgacag cgggacgccg ccgatggttg cggagagcgc
     4801 cgccacgtcg atgtctccat gatcgagatc ggcgacgagc ggcagccgga agttgctgcc
     4861 ggtgatcgtg accggcgtct ctgcggagct cgtccccgac ggaggatcga tcgacgacag
     4921 tgcagggccg ctgccgggcg tcgtacaggc agcaatcgct gcgacgatga tcaggctacc
     4981 aaccgaacgc accgaaccac cactcgtagc ccgcgacgag gccgagtccg tcgagctggc
     5041 caactgcatt cgcagcttcg agcttcgcgc ggccgtacga cgcgcctgcg aacgcgcgcc
     5101 ctgggccgag cctcacgtca gcggtgagct gaccgcggaa cccgatcacc gtcgcggcgt
     5161 cgcgatcggc gggctggagc gtcggcgtga gctcgacctt cacgcgcatt ggcacgacac
     5221 cgcccgcgag cgtgacgccg agtcgccgcg tgatgcgaac atgcgcgcgc gcgagcacgg
     5281 gaacgccgag accttggatc gagcgatcag tcttgtcggg catgcccatc acatcgacgg
     5341 tcgtcgactc gctggtcatg atgccttcga cgcccgcgct gaggccaagc tcgacggtgc
     5401 cgccgcgacg caggccgaga ccgagcgcag cgcgcgggct cttcagcgcg ccgaggttgt
     5461 ccatccagcc gccggctgcc cacgctccga cgacaaacgc gtgcgatgga ggatcgaagt
     5521 gcacgctcgt ctgcgcgagc gattggccac cggcgcgcac gacgatgtcg gcatccgccg
     5581 catgctcggc agccgcgacg cgcgcgcgaa atccgcgctc ggtgcgcgtg acaccgcgaa
     5641 cggcgccgcc ggtcaccgcg agatcgaacg tgccgtcttg cacgaggttg ccgaacttgt
     5701 cgcgcacgac gatcacgagc tcggcatcgc gaccatcgac atcgatgctc accgtggcgc
     5761 tcaccgcggg cgacgacggt ttgagatcca gcgactgctg cacgccaccc gcgtcgacga
     5821 cgagcttcag cgtgccgctc ggcggcagta cttctggaat cttgccgtgt acggcaccgt
     5881 cggtgatttg tacgttcgtt ccgccgagcg tcgctgtggc cgtggagcca tcgagcacgt
     5941 tgcccgatgc gtcggtcatg tgaatcggga cgacgagctc accgccggga acgacgggcg
     6001 acgtcggctg accgagctcg agcttcgacg ccgcgccgcc gccgatttca acggggactt
     6061 cgccggtgcc cgcattcgga tcaccctcga cgcggatcga aaccctctcg ttgccgggtg
     6121 tcgtcggtgc ggtgaattcg acggtccaca gaccgccgcc cttcgactgg attgcgccga
     6181 gcgcgccctt cgacgcagtt gcgacgacct tggcagatgc cgcagcaggt cgcccatccg
     6241 cacctgccac tgcgatcgcg atcaacgtct tctcgccggc agcgagcttc gtacttccgg
     6301 caacggcgcc gatgcgaagc cgcacgggaa tcttcaggtc ggtgacggca tccgtgacgt
     6361 agccgcgctg gctcgtcgat cgtgcgaccg catgcgtgat gccgggcggc acttcgatcg
     6421 gaacctcggc ggtcttgccc ttcgtcggca ccggaccgaa ccacgtgccg gcgatccaca
     6481 cctcgacgtt cgaattcgtg tccgactcga cgggcaacgt cgtcacgccg atcaccggaa
     6541 cgaacgccgc gccggcgcgt gtgccatcga gcatcgcgat cgcgacgagc cgcgggacct
     6601 tgccactgac ggagagcgtg agcgcagcgc cggcgacgtc gacggttcca tcactcgcgc
     6661 tggctttcag cgcgccaggc gcagacgcct tcttgccgtc ccacgagatg acgggcacct
     6721 cgatcttcgc atcggtgctc ttcgccatcg ccgcaacgcc cgcatacaag cccgcaggtg
     6781 gcggcgtgac cttgagcttg aagctcgtct ccgcaccgcg actttgtgcc ttgcactcga
     6841 gcgtcgtcgc cgcggtcgca accggcgcca gcactgccgg caatccattc ggcggcgctg
     6901 cgagacctgc ggcattcgcg cacgtcaccg tcgcgacatc gggaacgggc agtgccttgc
     6961 cttgcacttt cggcccaccg gtcacggtca ccggtgcgaa cgagcggccg tcgccgacga
     7021 cggagtccgt gaccttcacg tccactttgt cgtccgcatg cgcgagctgc gccatgctcg
     7081 cgacgacgat cgcagcagct agcgcttttc gttccacagg ttctccgtgt gagctttcac
     7141 gcgctgcttg cgaatcgtga tctcgcgcgc cgtcatgaag atcggctcgt catggccgtc
     7201 gacgaccgcg tacacgcccc agtgatagac gccttcgcgg agcgagccga cctcgacttt
     7261 ctggccactc gacggaatcg tcacgatcgg atccaccgac ggtaccgcgc cccgtccgac
     7321 gacgagcttg tactccgacg catcgccgag cgactgccac gagaattcga tcttcggata
     7381 gctgtcggag aagccgacct tcgcgccgtc gggcggtgtg agcaggagat ccttcggagg
     7441 ctcttgctcg aagtagatgc gacggccaaa gccgtactcg ccgaggtgat cgctcttgtc
     7501 gcgcgcggcg acgcgccacg cgtagacgcc tggggctttc ggtgcgaact gcaccgccgt
     7561 cgaggtggcg tcctcgttga gcacgagctg gcggaactcc gtgtccttcg agatctgcac
     7621 gcgatatccg cttgcctctt tcacggtcgc ccactgcaga tcgatcggct tgtcggcgac
     7681 gaagtgaaac cgcgcgtcga tgccaggcgc gacgctcttc ggaaacgcga tcagagacac
     7741 cggctcgctc gccgattgct tgacgacgcg cgtgaccttg ccggcggtca ccgcttgctc
     7801 gccggcctgc gtcttcaccg cgacgtcgcc ttggatcgct gcgatgtcga gcgagttgtc
     7861 ggccgcgggc gagatgcgga acgtagacgg ctggctcgcg cccgacggcg ccgcgatgtg
     7921 cgcttccgat ccgtcggccg ccttgacgac gagcgtcgcg cccggatcca cgacggccat
     7981 gaccgtgccg tcttgcaagc tcaacgcatc gtcgatgatg accgtcgtcg gctcgctgat
     8041 ctcgagcgtg ccgcggcctg cgagcttcaa tcgcacgaac gactcgtccg ccgtccggac
     8101 cgcgtcgcct gcgtgaagcg cctcgtcctt ttcggcgggc gtccactcgg tagcgccttt
     8161 gcgcttcacc ttgacgttgc cgccgagctc cgcgacggtc gcaagcgtgg gcacagacgg
     8221 ctccgcgggc ttcgcggttt ccgcggaacc attgctcgcc gccggcggcg ccttgtcctt
     8281 gtcgtggccc ttgcaagccc cggtgaacgc gacgcacgcg atcaccaccc gaaggtgcat
     8341 gcgccgatca taccctagtc ccacagccag acgtcgtcga cgtccaggtg tccgtccgcc
     8401 atggtcgcgt cgtcgacgag gaccagggtg ccccacttac cccgtagctc cccgatgtcc
     8461 cacgtcagct cgcgcagcac gtcgccgccg agcgagggcg gaccgccgct cttcgcgatc
     8521 tgaccgtcga cccacagctc gacgcgaagc tttgtcgcat cggtgccccc gccgagcttc
     8581 agcgacagct tcgcaccgtc gagcgcgaac gaaggcgacg tcacgcggcc cgtcgctgaa
     8641 tcgccgccgt gcatgctcgt cgcaaagcgc tgtccggtcg caccgatcac gagctcctgg
     8701 ccgggctgcg actcggcctc tgggccgttg ccccacgcac cacccgattt cgtccagccc
     8761 tgccaggtcg cgccctcgaa gtcgaacacg accttcgcgc ccttcggtgg cgccgcagcg
     8821 agcgcgggga cccagatcga ttcgggaacg atgagcgcgc ctgtatataa gtgtggctgt
     8881 tcgttggcgg gcagcttcag cgcgggccgg tagtacgtcc gcacctgcgg cagctcgagg
     8941 aagagatcgc ggttatcgaa gatgatcgcg ctgaagcgat gtgactgcag cgcctcgtcg
     9001 agaccgagca ccatccggtt ctgccgccag gtgacgtcct tgatgcccat gcgatgaacg
     9061 tgcggctgct tgcccgcgag cctgaggtac caagggtgcg atggcatcca cacgtcgcct
     9121 tgcagcgccg cgatgcgttt gatcagcgcg tcaccggcgg cgacgtctcg ttgcgtcggg
     9181 atgaatcgct gcgggttcca ctgccagtgc caacaagtga ccgcgagtgc gatcgcagcg
     9241 accgccgcaa tgccatgcga gacgaggcga gggaagcgac gtggtcccca caggatcccc
     9301 gtgcatgcgg cgagcgcggg aatcgcagca cctgcggcga gcgcaccgtg caggaacgcc
     9361 ggcatgtacg cgttgaagtg agcgaactcg gtgccccaac cgatcgcgcc gacgatcgtc
     9421 gagaccccga acgtcgcggt ccacagcaag aacggacgcg tctggcgtgg cagctcgcgc
     9481 cgaatgcctt cagccggcct gcgaagccag gtgacgagca cgaccacgag cgtgatcgcg
     9541 acgatgatcg tcatcgcgcg gaagtgccag aggatgttgc cgtacgactt ccagaagcgg
     9601 tcgtagttga aatcgtgcgc gttgtggatc ttgcggacgt agacccagaa ccaaccgccg
     9661 ctgccggtat tcatgaggcc ggtgccgccg aaaccgatga aggccgcggt aatggcgtac
     9721 gtgccaacgc ggcgccacgc catcacgagc acgacgagcc cgccgagcgc gacgtagacg
     9781 atgccggtct gcttcgcaaa gaacgcgaac gcaaagatcg ccgcaagcgc gatgacgcga
     9841 ccgtggccgt tgatgccgat gccctcgggg ctccagaaca gcgcgcccat gaggccgaac
     9901 gtgatcaaga acagaaacag cgtgtcggcg cgaacgagat cgaaccagcc ttcgacgaac
     9961 ggataggtcg cggcgaagag gccaatcgcg agcacactgc cggcgagcgc aggccaccag
    10021 ctcgtgtgac gaaaccgcgg cgcgcagatc gacacgatgc cggcacctgc gatgccgacg
    10081 agcgacagga ccgacaccac gcgtccgagc gtgtacgtca gcccgaagat gccgccgagc
    10141 gcggcaagca gagcggggta gagcggcgtg tagagatacg ggatgaactc gatcgacggc
    10201 gcgttgtaga tgcccgtgcc ctgctggaac cgcagcgcat ggtgcagcat cccgccttcc
    10261 atccactcga ggtcgtacgg atacgagatg cgtccggcga tcgcagtcgc gagcaatagc
    10321 agctgataca gcgctgggat cgtgaggacg atccagccca gtgcctcgag cgcgcgtttt
    10381 tgcggcgtcg tcacggactg atctcgattg tcgcagccgc gaccgcgttg ttgtcgagct
    10441 ttgctgccac ggacttcacc ggtgcgcgtt cgttaccccg gaagaacccc acccacagag
    10501 tgtatggccc gaccggtagc gttcggggga gcacgagtgt cgtcgtgtag cgaatgaact
    10561 gcccgggctt ccaccactcg aacggtcgaa ccggcttgtg atcgccattg acgaatgcat
    10621 tcgccggacc cttgaggtgc gcgaatatct tccagtcgtt cgacacgcgg cccttcgctt
    10681 cgaacgtcca tgtcagcgtg atctggtcgc cgggctttgc gcgcgcgggc gcctcgacgg
    10741 agagcaggcg tgcaccagag ccgatcgtcg cgtcgagcgg agcgggtagg cccgcgccgg
    10801 atggcagcag cgcctcggcg gcgccgacgg ggacctgcgc gacgtcgtac cagcgctcga
    10861 gcacgtggcg cgtctccgtg cattcctcgc caccttcgag atcgtgcgcc tcgagtggat
    10921 cgcgatcgac gcgatagacc tcccagctcg tctcggggct gacgttgtag atgacgtggc
    10981 agcgctcgtc gacggcggct cgtttctcgt tgttgttctc gaacgagagc tgttgaaaca
    11041 cggtgcgctt ggtgtcggca ccgccgagga catcgacgag cgagcgcccc atcatgtcgg
    11101 gcccgggctg accacctgcg aggttgacga gcgtcggtgc aatatcgatg tgaccggcgg
    11161 gcgtcgtcgc gtggcgcggc ggcaaccccg gcacgcggat gatcagcggc actttcgtct
    11221 gcgccgaata caggtggtag ccgtgatgat cgatgcggtg ctcgccgaag ccctcgccgt
    11281 gatcgcccgt gatgatcacg atcgtcttgt cgtagaggcc cttcgcgcgc aggtcgtcga
    11341 acaggcggcc gaggtgtaaa tcggtgaagc ggatctcgtt gtcgtagagc gcgaccggat
    11401 cgctgccgaa cgatggcacc tcgggatggc cctcgtagtc cgcgtgcgga tcgtagtagt
    11461 ggacccagag gaaccacttc tcggcggcgt gtcgatcgac gaatgcgatc gccttgtcgg
    11521 tttgctcctt cgacgagctg cccttggagt gctcgggacc gccgacgtcg ccgtggagcc
    11581 ggtagttctc gttgtcgtac tcgccgaaac cttggtccat gtggcgatcg cggctgaagt
    11641 accagaagtt cgtgatcgcc ccggtgatgt agccgagcgg cgcgaggtac tcggcgagcg
    11701 tcgtcgcttt cggtgcgaga ccgggccagc cgccgacggc gtagtcatag aacacgtcga
    11761 gaggcagccg gccggagagg atcgcgggca tcgagtagcg cgtcgatgga gcgtgcgccc
    11821 agccatgatc gaacacggtg ccctgcgcgg cgagcgcgtc catgttcggc gaagtgggtc
    11881 gcttgtagcc gtacatgccg aggtgatcgg cgcgcgtggt gtcgatcgtc acgagcagga
    11941 tgttcatgcc cggcggcacg gtcgcgggta cgggcgcgaa cgcgacatcc tcgtgtgtcg
    12001 ccttcatgct cgcgtcgccg ccgacgcagt tctgatcaat gccgtcgtcg gggatctcgg
    12061 gcgcgccggg atgcacggtg cgatcgccat cgtcgcaatc gccgccgccg agaaaccgcg
    12121 agtagccgtc gcgatcgcga tcgaatgcct tgcgaacgcc gcgcgcgatc ggcgcaccga
    12181 ggcccgtgta cgccgtcgac gctttgatga cgggcgccga gccaccggcc ccgagcacga
    12241 ggatgaacag cgcgatgggc cagacgaccc agatcgtgcg ccggatcgcg ggccggaaca
    12301 tgccgatgaa gtcgcaagcg gtcgaggcgg ggcgatacgc cggaatcgcg agcagcgcgc
    12361 cgaacacgaa cacgagtggg ccgcgcagcg gcaggatctt cgcggtcgtc cactgcgcgt
    12421 gcgcgagcca cgcgaggatc gcagcgatga gcacgccacc gacgacgagc ggcgcggtcg
    12481 gctcggcgat gatcgggatc cgcttcgcga tcttgcgcag cgcgagctcc accggttgcg
    12541 cgacgacgaa catcgcgatg atcgcggcga gcagtgcggc gatcgtcgcg gcgatcacgc
    12601 tgagcacggc gaggcccatc gcgtggctct tcgcgacgac cggcgtcagc atgcggaaca
    12661 cgacgtagat cgcgagcgcg acgagcggca cacccgggat cacgagcgac atgccgatga
    12721 tcgcgtcacg cgggtccttc tcctggcgtg cacgatgctc gccccagcca aaccggacga
    12781 gatcgccaag ccgcgtgcgt cgcgatagat gtcccaggat cgcggcgccg ataaatcccg
    12841 cgagcgcgcc ggtcgctgcg agcgcgaggc cggagtagac gacgaaccgc acgcgcgaca
    12901 cgaacgacgg cacgaattgc gccgccggtc cccacgacca cagcgcgtcg atcagcccgg
    12961 ccgcgagccc ggcgaacaac ccagcgagcg cgcccgtgcc gagcacgtgc gataccgagc
    13021 gggggcctga cgccatcgac gccgtgacga taccaccccg tgcatgccgt cgcggtatgg
    13081 ttggccagtg acgaccgatc gcctgcgctg gctcctagtg ggagttgccg ccatcgtgct
    13141 cgtccttcac gcgttcgcgt acaacttcat cacggacgac gcgtacatct cgttcgtcta
    13201 ctcgcgcaac ttcgccgagc acggcgagct cgtgttcaac ctcggtgatt acgtcgaggg
    13261 ctacacgaac tttctatgga cgctgctgat cggcctcggg atgctcgtcc acatcccgcc
    13321 cgagtggtcc gcgaagatcc tcggcgcgct attcgccgtg ctcacgctgt gggtgacgtt
    13381 cgtcgtcgtc gagcgcgcgc tcgggcgcaa gacgctgtgg gcgacggtgc cgccgctgtt
    13441 gctcgcgtgc tcggcgggct tcgcgtgctg gacgtcgggt gggctcgaga cgcaactgtt
    13501 cacgatgctg tgcacgatcg cgatcgaagg cctcgtcggt gcggaacaag aagatggagc
    13561 gagcgggctc tatcgtgccg cggtcgccct cgcgctggct gcaatgacaa gacccgaggg
    13621 catgctgatc gcggcggcgc tcggtgtggt gcgcatcatc tgcaacgtga gcagcaagcg
    13681 cggcctcctc ggcaagaccg agctcgtcgc tgcgatctgc tttctcgtct tgtgggcgcc
    13741 gtggttcgcg tggcgatgga actactacgg ctatccattc ccgaacacgt attacgtgaa
    13801 ggccaccggc gattgggcga gcccggagat ggcgcgccag atgcgcgaag ccggcatgta
    13861 ctacgtatgg atctggctca agcagacgaa gctcgtcttc gcgctaccgc tggtcttcat
    13921 cggcctcgtt ggcgctcggc ctcgcacgcc tcggttcgcg ctcgggctgt catgcgcgct
    13981 catcgccgcg ttctacatcc cgtacacgat ctccgtcggc ggcgacttca tggggctgca
    14041 ccgcttcatc atgccgctgt tcgtcgtcgc agcgatcgcg gtcgtcctcg ggctggagct
    14101 cctgtgctcg ctgattcccg agtcggcgcg caagtacgtc gcgctcgcgc tcggtctcgc
    14161 gctcgttggc atgttcgctg gaacgcagct gcagctcacg cagaagagcc tggcgtgggg
    14221 caacttcaat cccgatcacg gcatcgacac gccggcgttc ctgatcacgt acaccgagga
    14281 ccgcgcgctg atcggtcagg cgatgcagcc gtgcttcaag ccggacgact tcagcatcgt
    14341 cggcggtgcc ggcgcgcagc cgtactacgg gcgcatgcaa gcgatcgacg tgttcggtct
    14401 ggtgtccgat cgcgtcgcgc atcgcgagaa gcgcatccga ccgcgcgcgg gccacacgaa
    14461 gttcgcctcc gacgcgctgc tcgccgagta cgagcccacg ttcatcttca gctgctacgc
    14521 gattcatccg acgccggcgc ggccgacgac gctcaactgc aacactggat tctggctgag
    14581 ccgtggctac gagatggtga cgatgcacgt cccgggcatg aagcagagcg gtgagtacta
    14641 cacgttcctt gcgaagaaag accgcaactt cgaatgtccg gggcgcgtac cctaatcact
    14701 cgctattggg gagcgattcc cgtcgcgatc gcgatcgcgc tgttcgtggt gcgcgcgcta
    14761 ctgcacaccg cgccgctcga taacctgtcg ccagcgccgg ccgaggcatc ggatccaccg
    14821 ggcacgcgcg cgtatgcggg ctacgtgatg atctcgcgcg gcggccccgt catcatcggc
    14881 tttcagagcg atggcccggc gcggctcgtc gtcggcacac accgcctgat cgcaggccgc
    14941 ggcctcatca aagatcgcgc ggtgctgcct gcgggaccga cgccgatccg attcgtcgcc
    15001 ccaccgggcg cgcggctcgt ctggaatcca gttggccgcc gcggcgatcc ggaatatttg
    15061 ccggcgagct cgctgtcccg caccccggac gggtttggtc acttcgtcgg cgcggccgtg
    15121 ctcgacggag tgatcgcact gctcttgctg ctcacgctcg tcggctcgct gctctatctc
    15181 gcgcgcgggc gattgcgggc cgttgactcg cacacgtggc tcgcgatcgg cggcgtcttc
    15241 gccggtgcgc tgctcgtccg cgtgatcggg ctcaacgcct tcggtcagac gtgggacgaa
    15301 gacgtcaact ggggcgcggg gcgcaactac atcacgaaca ttctgtccgg cgatctcagc
    15361 gagggctcgt ggatctggaa cttcgagcac ccgccggtga tgaagtacct cgaaggtatc
    15421 ggcgcgcaat tcgccgacgg ctttggtccg gcgcggttcc tgtcggcgat ctggacgtcg
    15481 ctggggtgcg cgctgctcgt gccgatcggg atgcggctct atcggctgcg cgtcggcatc
    15541 g
//
LOCUS       DATLBZ010000072        56606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8998996, whole genome shotgun sequence.
ACCESSION   DATLBZ010000072 DATLBZ010000000
VERSION     DATLBZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 56606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56606
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8998996"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VMZ53_03510"
     CDS             <1..640
                     /locus_tag="VMZ53_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27542.1"
                     /translation="EDDPTLRDRLRNAPDALTRASILAAVNATLLANGITDPAGLVEL
                     PKHGAHSRTWTLPTGTGGTFAVAGTTVTFTPAVLPWPRKPYRSPVLVPFGQMQLFLSG
                     CANAANNGLHVITGLDGNAAVWTDASAVAGIDASCSWQLRTYNAEGFLQDGEGRAFSR
                     GGYRSARLVPLRIVIILPFGSTAGIEASVREALRTKKAAGIAVTVERRTIAP"
     gene            689..1897
                     /locus_tag="VMZ53_03515"
     CDS             689..1897
                     /locus_tag="VMZ53_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27543.1"
                     /translation="MQFNPDGEGLDFTDMQNMQTFAQARNWIALFTHALANAAGDPSA
                     PGDLDTPEAFAAFNELSMYGGAVIALHPGTAWVGAGGANQLAIFPGSIGLVMDEPFNT
                     GDDFGLFRVTGVLALATAVGAAQPRIDIVEVKLTHIDGDPQDRHFEDATTREPSTQST
                     SKEKQLQLTYQIKAGTPAANPTYPTPSAGFTAMAAVYVPALHNAVHSPTNIRDLRMPV
                     GVRCVDVDAKAMFFTGANPWAVVDGMIAGAAAASDPDADRVIAPCPISTKSARLLAVA
                     VHGFAFDPRCEIGRLDYPTAGGAATFTKYGDLVNVSGDMLQDNMVTVDGMSLADEIAD
                     VAVIRGVRAAGERVGTALWCNGYAGGMAHPGVPMNDGAEATVSKLAISIGGANGSRVG
                     LVRFWIAEGL"
     gene            1955..2290
                     /locus_tag="VMZ53_03520"
     CDS             1955..2290
                     /locus_tag="VMZ53_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27544.1"
                     /translation="MPVDWLVTLWEKGGVALVVIVLLFLLARFVGKRGVTAFDLMAKA
                     QTDSMAKIADAVQTSTAANVVALGGLGERVSRMEGTVELLGKLALQHGAEPQAQPQLR
                     TVQRPPSKP"
     gene            2349..2714
                     /locus_tag="VMZ53_03525"
     CDS             2349..2714
                     /locus_tag="VMZ53_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27545.1"
                     /translation="MSRIVTALIEPMQAKDLLPMRRHLQAITASHEPGTAPPDHDHPI
                     TKALVDRLPKMTYGELEAARERLQMSSGDDGGKKWISSSRDRDGWLRLVNAELQLRDH
                     ERERAGDSLIDFLTKGVRQ"
     gene            2711..3169
                     /locus_tag="VMZ53_03530"
     CDS             2711..3169
                     /locus_tag="VMZ53_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27546.1"
                     /translation="MTAIAGKTDSHENSLLLLIFNNTTYAKIGDATGIVGSTVPGSLY
                     VSLHTADPTEEGVQNTNECTYTSYLRVAKARSGAGWTVTGGSVVPASAITFPAGTGGA
                     GTATFFGVGCSTIAGGAADPLLYAGAISPSIVTGNGITPQLSTATAITES"
     gene            complement(3082..3606)
                     /locus_tag="VMZ53_03535"
     CDS             complement(3082..3606)
                     /locus_tag="VMZ53_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27547.1"
                     /translation="MAPLVLTVAFPVLPANSVSVTVFAVVAVPSVTVMTVPVCNAPTF
                     WLVVQIEPCTPAAAAPLSNTTAVLGATVHVLPIESVTFVMSFVAPPVELLSAAIAIAT
                     NAPIVQLVAGETVNVSALTLCVPVPRFVIEMVTRTFHPPRRLLDHDSVIAVAVLSCGV
                     MPLPVTIDGEIAPA"
     gene            complement(3700..3879)
                     /locus_tag="VMZ53_03540"
     CDS             complement(3700..3879)
                     /locus_tag="VMZ53_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27548.1"
                     /translation="MLVVMSYDCVLFAVVWTLACEMIALPLVVDPILLVCAVDHEPLV
                     ESLSPVSVIVVPAST"
     gene            complement(3892..5217)
                     /locus_tag="VMZ53_03545"
     CDS             complement(3892..5217)
                     /locus_tag="VMZ53_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27549.1"
                     /translation="MPENREIPSELLGCGRVAIAPVNCEIAIEPLGPGWAAIVPATPV
                     LRLGPGAGTGGSLSVAAAAVDVPPAVPIATAIEPPTADAAVCPAEDPIALAIAMPIAL
                     AVDAPAASPLASAMATPIALAVDVPAAIPTLYPAATASADDEPAAAPVDCATDDPVPE
                     AVDEPSIDPDAAAVEPPVALALEEPPVAPVDSADEDPVAAAVADPAAVPALVAVDDPT
                     ASAADVPSLDPVAMSSAPPAADAVDEPALDPEDCAVDAPIALADDEPAPAPAAAAVDA
                     PMAATVEVPDEDPEPIAVDEPAALAEDAPADAPAVITSDVPVALAVDAPAAVPEPTAD
                     EDPTADAVATPAEDPLDCAVDDPTPLAAPMPPDDPDATTIAEPEPDAVDEPALDPADA
                     AVADPTAAAVLPPDPDPLAAAVDEPTALAVDEPALDPTYGVSCTMIHDA"
     gene            5267..5641
                     /locus_tag="VMZ53_03550"
     CDS             5267..5641
                     /locus_tag="VMZ53_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27550.1"
                     /translation="MTPIELWKGESRTLTVTVVHPDTGFPVDLSAGTWQIDTLEFQIK
                     SDVEAADPALVSKSLVGGITLLTQSGDTLGQAEIEIDPADTQSITPGVYKYDVVAVFT
                     NAKRVFVIKPSDLQLYGVVNGL"
     gene            5650..6042
                     /locus_tag="VMZ53_03555"
     CDS             5650..6042
                     /locus_tag="VMZ53_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27551.1"
                     /translation="MIAVSLAVAAIAGAFVWFGVALRNAQQTAADARVDAANKAGRLA
                     IAAADVATQKNRADDEKERADALDQELETVAADGDAAGARERVLRRKARAASADSTAR
                     DDSGEVRGGRPDPAVARRPDDDLAKPGD"
     gene            6179..6697
                     /locus_tag="VMZ53_03560"
     CDS             6179..6697
                     /locus_tag="VMZ53_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27552.1"
                     /translation="MSRRAFVWIAFAVSLLFAPLAWSDTPSDAGVAAVSAGSALEGSG
                     SAAGVTQPDVVNPAQHPAQALDEVKLARKTSWPLAVWLALAMAGKALAYGRDKLKRVP
                     VVGGVAALLAKGKGAMIVAAVGAVGAAGYAVLLDGGTLTAALVASVVALAGVTRSTTK
                     TTGGDPAPSSPS"
     gene            6688..6763
                     /locus_tag="VMZ53_03565"
     tRNA            6688..6763
                     /locus_tag="VMZ53_03565"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6721..6723,aa:Thr,seq:cgt)
     gene            complement(6795..7367)
                     /locus_tag="VMZ53_03570"
     CDS             complement(6795..7367)
                     /locus_tag="VMZ53_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27553.1"
                     /translation="MLKPAEALARMRSMVGLDFPYILGTGNFSGEPPRGPWDCAGAIC
                     EAFKVKRHRPGFARGPLPPAWASLADVVDDINTNSMIKDAVVRGELFTFVPFGDPLEG
                     GDVLAYPTIRLVVDGERHEWIGHVQMIDDPKGALSGGPYSDAWVLHSHGPNGRHPAVT
                     RNKAYAMDVHNHSWSKLGHKAHALRAVTAP"
     gene            7492..7857
                     /locus_tag="VMZ53_03575"
     CDS             7492..7857
                     /locus_tag="VMZ53_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27554.1"
                     /translation="MANNTNPDHEKFDFAEFFAEFGKGATNRVASQRMQEIVKACSET
                     GRKGKIVITLEVGAVGGVAELRAKISVTKPEPQLPGGVYFATEDGGLVEEDPRQLKLP
                     VARVIDLQPVKNINKEQGQ"
     gene            7878..8750
                     /locus_tag="VMZ53_03580"
     CDS             7878..8750
                     /locus_tag="VMZ53_03580"
                     /inference="COORDINATES: protein motif:HMM:NF021574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HUS27555.1"
                     /translation="MTTAREGMDVNTRITEWGHPDAADDDSGPAVPVVLVTEVDNDGN
                     AATRVTLAGDILAELDRRMPGPRRREGRVQLQDVQSFCEYLSRYQRPESSVVYANVSA
                     MTFEAVIDEHPLGLPNDEIATAWRGHRAIYACPRSPEWLAWTAKDGAAMKQDEFADFL
                     ETRLEDMVTGEGFPKPVEVLAVARQLHIRTKGTFQREINPTNGDNILVHKTETETGST
                     QIPRAFAVAIPVFEGGERYQVEARVRFSIGDRGPVFSYTLHRRSEIERDAFGGVRKMI
                     AGTTKLPVLSGIPG"
     gene            8874..10079
                     /locus_tag="VMZ53_03585"
     CDS             8874..10079
                     /locus_tag="VMZ53_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HUS27556.1"
                     /translation="MVATAGQSNLSPEQLAERAQGITATDVAAIVGVHPYRSAVDVWS
                     DKLGLSAPFVGNDRTKWGNILEPVIRSDYEERKGVRVETRGTLTHHERTWMKATPDGL
                     VYVGSDAEPDRGLEIKCHTMHLAYLYGSPGSDEVPQYELCQVGWNMAVTELQRWDLVA
                     FIDGQPTDYVIDRDDELIDMLRERAERFRHDYVLAKTPPPPDGSEAYTAWLNAQHASD
                     NALAALVDVSGDVETMRNVERLRTLRDEIVALEAQEGLVVQSLKARIGDHAGIAWPNG
                     KPAPKKGKLAGQLPVDKITWKKSKDGVRTDYAAAFHALKARAQLVASANADAAHRAIT
                     ALDKLADQYFANTRATTTGKEIRATLEQLRDLAVEVAHAEPATAPAPGSRRFLVPKHW
                     KGTADSADE"
     gene            10120..11430
                     /locus_tag="VMZ53_03590"
     CDS             10120..11430
                     /locus_tag="VMZ53_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27557.1"
                     /translation="METHDDSTEHEYDSAPRGNQLARRDFSGSSLSVASAATDALVAK
                     ERANVEARWIMAMRRPRNMDDVRQLMLKECRRPGFADAAVYEIPRGGTKITGLSIRFA
                     EVAVRCMGNLQPEVVTIHDDDDTRIVKCSITDYESNITWSRDISIRKTVERKQLARGQ
                     RPLGERTNSYGDRVFIVPGTDDDVATKEAAIVSKTLRTLILRCIPGHLQDECLALCNK
                     IYADKAAKDPDAERVKVLDAFAGIGVLPSEIEELIGHTTERMSPAEVDRLRKLFSAIN
                     EGHASIAEAIDAARADRAKAPKAAPAQTATPGDEGGKQPTSAQAAPQATNAAPAQQPA
                     TAQPAAPASAGQAKASKSGKGTAGLKNALGGNPDSSFAQAAEQARAKDAAREAKADPE
                     AEPGWMSGKADDASEERPCAGCGVPIDAKKSDPPGGKCYACLNA"
     gene            11541..12194
                     /locus_tag="VMZ53_03595"
     CDS             11541..12194
                     /locus_tag="VMZ53_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27558.1"
                     /translation="MATQTLYAIRNNLTGTILTGADGKIATWADRSMASLRAREYGLG
                     FGSFAIETEPAPTDSDLVTVREAQPEEPGEGIPPAPWLEEGRGVLRTAVADRCASCKL
                     PAHLTDVPNTNEQHPALGLACFACRDTHDTGAKLLAMLSAAPSCQLSRPTMARKLGRT
                     ASSIDRALAYLQRERLIERAYKSPNIEKPSATTYKLTDAGSRAHFANTEHKEQHAHA"
     gene            12181..12621
                     /locus_tag="VMZ53_03600"
     CDS             12181..12621
                     /locus_tag="VMZ53_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27559.1"
                     /translation="MHTPESPFPFTTKSGAVIVDERCSCGALRSQHETTIAYGHGPRV
                     VAGAVTCSRYTFVRWVFTSRALPAGFEALDDKHAVRIERASKRDVGASGWRYEVRDMS
                     AKLGGAKLFTCKAPKARTSLDALRHAREELRIHLERQPELGGIS"
     gene            12636..13673
                     /locus_tag="VMZ53_03605"
     CDS             12636..13673
                     /locus_tag="VMZ53_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS27560.1"
                     /translation="METNGIANNARQRLGEVIRHYREDRDITKREVSRRCKVPEGLVD
                     RWEGGEAVPNSDEWKALCRGMNRALNAYQELYLRARKEADDDARSRAVQTMSNEKPKE
                     PTKMQTPTPNNPSARISTSLGDKLAAATAPKPAISIVRDPDPEPAPPSDTTSRTTRAA
                     MAKLPKGWKSHEMLAKRRAFALDILRQRPNIRDSGADSLEELLQRAFGVGLANYTVKE
                     LREQAKREADGRPMPVASPPPMQWHPPIVQPQAPVTHAEAVEAQRKATAAIASPEDIA
                     RMHGAKPAPVSTTPTSAGDIETAVQLVLEALPNLQTFTIHVDEHGEASVDYQIRKVVI
                     TTDGGSFKVKR"
     gene            13676..14005
                     /locus_tag="VMZ53_03610"
     CDS             13676..14005
                     /locus_tag="VMZ53_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27561.1"
                     /translation="MAQRLKAKAEAITVRKALGRSPLARPAFTEPAPPLELTIETTDA
                     QRLADAEVALEQPKIARHMLEDARTRLAAAGAGGPHPAAIAVARSLAETYKAIVGARL
                     VVRTVKR"
     gene            14072..14506
                     /locus_tag="VMZ53_03615"
     CDS             14072..14506
                     /locus_tag="VMZ53_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27562.1"
                     /translation="MTNNITIHGILAGAYRGKGLERTLLTHASADGGVTALCRTIKAD
                     SLCDEVLDEAPSCARCERKVADRRKLPRFNGPQSYTSHQRYWHCRVLEAHRDVYITPG
                     IGWCGPGWQIVDALMARIAAGESFGPESWATALPSLVAAVAS"
     gene            14539..14880
                     /locus_tag="VMZ53_03620"
     CDS             14539..14880
                     /locus_tag="VMZ53_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27563.1"
                     /translation="MAAAFALSYRGQFDRHRPGCRTWRLFHTGVHTEYAGTGRVRFVS
                     FFRGAGYVVVLRSGRGRDGRPLAEPGWRPNIEIPGFTILVRTAKSSRRASYGTPVRHV
                     RITRNPNQPAC"
     gene            14898..15413
                     /locus_tag="VMZ53_03625"
     CDS             14898..15413
                     /locus_tag="VMZ53_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27564.1"
                     /translation="MERITEILHEGLTNTLRVHVPAPSPIIDRIARDASNNIAMALAS
                     EIGETLDEAHLQQIEHESLRTKLHNAGGHGATLDVMLTAVLERARLSLAARDAAQQLV
                     DAASELLEAFDEGDVDVFHPDDDDPVLPNATIECPEDDTCKCRGPAALNELSRACATA
                     RRELPHVERVQ"
     gene            15410..15730
                     /locus_tag="VMZ53_03630"
     CDS             15410..15730
                     /locus_tag="VMZ53_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27565.1"
                     /translation="MIPPSKRIWIYPDRTFTRPRDLEIIVLGEPEVIAPFMVELRNTI
                     ESWPGFDQVHPITMGEQLLGFRASRSKLAEHDDPVAEAMLMLKRFPIEWTGNGYGLGI
                     VGLW"
     gene            15799..16080
                     /locus_tag="VMZ53_03635"
     CDS             15799..16080
                     /locus_tag="VMZ53_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUS27566.1"
                     /translation="MRALPLTNKSRPGGRPRQTRAPRNPFADWLASCGKTPEQLAKAL
                     GVSVSSVYNARNGYFKPGRDLAVKIAEVSEGAVPVEAWGSVRARKRKAA"
     gene            16110..16397
                     /locus_tag="VMZ53_03640"
     CDS             16110..16397
                     /locus_tag="VMZ53_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27567.1"
                     /translation="MAFGDTRGPVTEVPSYVVKAAGGMGSPSKAHRPVARVERRGAFV
                     RVRVWFASEGAAVWFSPGYCTLSLPRFALLKARSWWRRWREPVPRAHVVQR"
     gene            16394..16648
                     /locus_tag="VMZ53_03645"
     CDS             16394..16648
                     /locus_tag="VMZ53_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27568.1"
                     /translation="MTDPGDENDHPETDAEWIAWLKRELARAADRGSRGQTLTPAQLI
                     GAQRGVLAYPITGGENQDPGDESDAPIRVWLRKLTVTVEL"
     gene            16645..17088
                     /locus_tag="VMZ53_03650"
     CDS             16645..17088
                     /locus_tag="VMZ53_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27569.1"
                     /translation="MSRFRYNRIGVPSIARPPERYIGELLEDYLAKWQAVREARVVKC
                     LADMLGHAREHTAIVEVPPRHAMSRSLCAYYGIDRTAHPESLAGWRSSNQEPFSIATF
                     REAMVRLLAYEPPPPAKPWITRQRAAQLEAEGVRRSLIEAVFEIY"
     gene            17400..17585
                     /locus_tag="VMZ53_03655"
     CDS             17400..17585
                     /locus_tag="VMZ53_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS27570.1"
                     /translation="MTLAVTVEAVVPLEVFMIRYALAVLAAHDGNKTHAAKALGVSRF
                     MLQRLCKRAATLGMAAR"
     gene            17585..18655
                     /locus_tag="VMZ53_03660"
     CDS             17585..18655
                     /locus_tag="VMZ53_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27571.1"
                     /translation="MRVNVDEQAINEPRIKRMARRLSKRHDRGIHHTEVLGRLIGVWM
                     LCYSRRSPVLRADDVDIAADLDGFAEAMITDEMADTTHAEDMIHVRGVSDRIAFLERQ
                     TERGRKGGRVAGRGPEQQAQARLFAEDEALAKRVLERTQANAKRHQERTLSERLRSPQ
                     AYSPDQAPDQALALDQDHVPTRGAGDVQQRQAGDPEKGSGLPVASAKPSDPTETAPET
                     ENRATGKPPREVPVEAVTLANVLLGQICSNHPSGRAAKMPPKVAEVTALRWAETIDKL
                     HRIDKFSWGEIEGMITWSQRHSFWRTVILGADNLRDKWDQMAAQRNRGATPRDTQPAP
                     NVFDVLDDYAKEKDAKDKEPTE"
     gene            18652..19248
                     /locus_tag="VMZ53_03665"
     CDS             18652..19248
                     /locus_tag="VMZ53_03665"
                     /inference="COORDINATES: protein motif:HMM:NF022858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative helicase loader/inhibitor"
                     /protein_id="HUS27572.1"
                     /translation="MTNTEITQALKLIFAAYPQQRARMSADDVKAMHDVWSLAFADVS
                     LEPAKAAIARHVCTSKWMPSIAEIREQLGTLHHGLKRSGINAWGDLKALRTYREREAM
                     EQVDPVVLFVCRAFDWIEWRTLLKASGDIEQWHVVVPLENEASDRARFAELYDKTIAD
                     ERTTAQLSAGGKIPAQLQRSRDGRQLGDIVSGLLPEKT"
     gene            19245..19412
                     /locus_tag="VMZ53_03670"
     CDS             19245..19412
                     /locus_tag="VMZ53_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27573.1"
                     /translation="MITVALLEALIALHVRKRPPMRRVLAFWRWRTELMRLEDARAEA
                     RLAMALQVSNG"
     gene            19405..19689
                     /locus_tag="VMZ53_03675"
     CDS             19405..19689
                     /locus_tag="VMZ53_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27574.1"
                     /translation="MDDVISLVMSTLVLLSSMVTQPQRASCPRSWVIGTGVRGGVFMC
                     QPDRIGCDYLDRVCEQPPGFVIGRVYCGPGLEPALTRYADGVRCQRQPRT"
     gene            19949..20254
                     /locus_tag="VMZ53_03680"
     CDS             19949..20254
                     /locus_tag="VMZ53_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27575.1"
                     /translation="MLNLSDKKVTRAQLVTEIEELRTRESLAREARQHLQNAIDEADA
                     KIANLEHKCAVLDTDVHNMLGTIRVMADASRAVRIRAKSVQCADLAIALPMRNTDTL"
     gene            20260..21186
                     /locus_tag="VMZ53_03685"
     CDS             20260..21186
                     /locus_tag="VMZ53_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27576.1"
                     /translation="MAQLSLGGLGGIVLAPDVLEEQRGAWCSPPEYTAAATERGPFDV
                     DPFTNPRSTLEAQYKCMLERGDDGFGLDRRDVPGTFYVKPSGIVQADCLKCYGGGRVL
                     GNDLEPTACPACNNGYHVATERTRVWIQPPYDLVLEALAHYGHTRFVALLRLDTSTEW
                     FEQLWNLSEVIMVPKRDRLEFVPPPGVKASSNPFPHGLYYKRAEDVPNAIRALCYPWP
                     CPAYPWASDPLGLRGSAAHQPVRWQAYTHVPATAKERPTTALHVAAVHGGAVASTPAC
                     GNPVAVHTQSAGGLQGIALACTVCLRMTERAA"
     gene            21183..21626
                     /locus_tag="VMZ53_03690"
     CDS             21183..21626
                     /locus_tag="VMZ53_03690"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HUS27577.1"
                     /translation="MIEQNLATVAAHWYRRIAMADPVASFVFEVVPIAKERPRTFHGK
                     RGPRTVTPERTKAAEEHLAWRFRLAMRGHTLEGNLGMVCVFYRPDRRRIDGDNMLKLV
                     CDAGNIARAWGDDCQITTKLARIELDAARPRTEIAIGPMASTLTR"
     gene            21637..22074
                     /locus_tag="VMZ53_03695"
     CDS             21637..22074
                     /locus_tag="VMZ53_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27578.1"
                     /translation="MADVSVCSVCKEPDDCRACACERSRIKDQPPPIPRALATAVWDL
                     VLEDCAHKPGPTWERVRRDMHERDQVGRERYGTPLTTNNGRDHLVDAYQEALDLAVYL
                     RAQLEEWADSDQRRHVLTIYTLHLVNVHHLRSVIDDKEQAHGR"
     gene            22064..22312
                     /locus_tag="VMZ53_03700"
     CDS             22064..22312
                     /locus_tag="VMZ53_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27579.1"
                     /translation="MGDDLARKLYEAWNVGGQHDLAWGLLETEKREQWDAVAVVARKH
                     MREQIALQLEAEVRDLDEDGGAYLRDGVRHAAVIVRPF"
     gene            22350..23204
                     /locus_tag="VMZ53_03705"
     CDS             22350..23204
                     /locus_tag="VMZ53_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HUS27580.1"
                     /translation="MGQTTGISWTDATFNPWWGCWKIADECKNCYADATAHRYTSQFG
                     ELWGRTAGRRFFGDSHWAELAKWNRKAERDGVRMRVFVGSMCDWAEMHPDDAIALQMA
                     NARERLWQEIRECSSLDFLMLTKRPEDAAQLVPWARGESYARDPWPNVWIGVTAGTRD
                     TLRVNASILRSIPAVVRFISCEPILEGIAADEWDDAIGPGHGKIDWLIVGDESGPRAR
                     PAQVDWVRTAREAALRHGVAFHFKQWAGRDVAGIGGRLPSTGAGNARKIHLPTLDGKQ
                     WAQFPKEH"
     gene            23209..24078
                     /locus_tag="VMZ53_03710"
     CDS             23209..24078
                     /locus_tag="VMZ53_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27581.1"
                     /translation="MSNAERYARELLKSVQNMIASALQPFDEQERRTAGLLAQTRDEI
                     GRDRQANHETERDLRATMRAILDRVESIEKDIKARDNYHKHERVRFENLVHKLDRDLA
                     TTADAQASSVDALAKRVALLESGRTGHMALRHGPPPGVPPFPIGSGGSERAIDRVFPP
                     YGEAFKPDPTTDAIERLARERDGLRVAAEEMRSAAMKAERECEEKQHVIVRRNERING
                     LTAEVTAAEKRAKDAERVLADRVNDGLRIADELRAKLTESSARIRDLEQQAQRRTGID
                     ERRLAKLAELEGK"
     gene            24112..24330
                     /locus_tag="VMZ53_03715"
     CDS             24112..24330
                     /locus_tag="VMZ53_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27582.1"
                     /translation="MEAAEADTIQTYTSARPFVPHEWVVRAVMKAAGGEVADPEQPEP
                     DDCQAGTCDCPGDDTHYPTRDPGSSGAV"
     gene            24364..24714
                     /locus_tag="VMZ53_03720"
     CDS             24364..24714
                     /locus_tag="VMZ53_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27583.1"
                     /translation="MRYEDQQLIHAYAKLGLEIAAAKNPQAQHDVSEAETLRRGYQAL
                     KRRMEQLSPKKKAMFRTMMRNEINAVRALMAKAEATGETFTLADISVEMIDAETGQAV
                     PGTPNVQLETESAE"
     gene            24711..25601
                     /locus_tag="VMZ53_03725"
     CDS             24711..25601
                     /locus_tag="VMZ53_03725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HUS27584.1"
                     /translation="MNLGTALVEPPIDEIERSDAWALVRTDSLRWLATRDPDSVDGFV
                     MDPPYSSGGQFRGDRTASVVDKYQQSGHAQGDYPEFYGDTRDGRGMLAWLSLVFADAW
                     RVTKPSGVIVTFSDWRMLPTMTDAVQAGGWVWRGIVPWYKPNARRQLGRFTGACEFAI
                     WGSKGPMPVERDVGALPGFFRDANVSPELREPPAYIQSNSVPGDDRQHVTEKPESVMR
                     ELVKLVTPGGLVCDPFTGSGTTGVAALLEGRRFVGCELSPEYFRVACDRFRALANLEH
                     RKDAARGQVGLFAQKQEQDT"
     gene            25598..25795
                     /locus_tag="VMZ53_03730"
     CDS             25598..25795
                     /locus_tag="VMZ53_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27585.1"
                     /translation="MTATATATPAGKPKRERKPPAAMTPTKAIADIVKLRAKALEAED
                     TILAQLPEADRKRVIAFLEAE"
     gene            25797..26711
                     /locus_tag="VMZ53_03735"
     CDS             25797..26711
                     /locus_tag="VMZ53_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27586.1"
                     /translation="MAVAKGADIPAARLLDAVEATAHALTAFEAWAGIKASAIEPGTL
                     HAVDEMQIRLAAWQNDRFGHVPTSDVHIALGVIEELGEAFDEDAGPEESIDALGDVMV
                     YAAQLCTANRLAVRPVIGLAVHYVKTNACHAHAIALAGQLAHVCLKHAQGIRGLGPFE
                     AYRPRLVDALALMIAKAIEDCAIGHELTVDPDGVFVVVGGEVLARKPGDTMIPKAPAG
                     MVNVQVMKEAIDAATEPFRRLAEGVDKLAEAEKVEPGDFQAPTFLRLTSSEKTEACPV
                     FDSASGVWCESDAQLRERINKHAADLSA"
     gene            26855..27424
                     /locus_tag="VMZ53_03740"
     CDS             26855..27424
                     /locus_tag="VMZ53_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27587.1"
                     /translation="MQLVTPTAWIAARTGAVVPVRPPMIYVAHPVAAQAGEAIATCTE
                     CDASHLFAPGDPLDLRLVCQHDAPIRNSSDPAAIVAFNCARALRWWRWLHLGLPDVTW
                     VMPWYVNVIANGEADPVLIERGLRDDCEVARRCDALMACGPRISSGMRREAIAAAEVG
                     NELFQIVGVHREPPVSWGATSTPWQRWAT"
     gene            27403..27693
                     /locus_tag="VMZ53_03745"
     CDS             27403..27693
                     /locus_tag="VMZ53_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27588.1"
                     /translation="MAAVGDMNLEQLLVWARAAAVESQSPYRDKEAARAILTLLGVAW
                     PCGLEEPPIVSMGDGTCGVLVDNGGDPLTPDELRGYAARLLQLAEEAEEQSK"
     gene            27690..28103
                     /locus_tag="VMZ53_03750"
     CDS             27690..28103
                     /locus_tag="VMZ53_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27589.1"
                     /translation="MTTQETSRESYRILIESGKLRGKQAQALEAVMRHGPATSGEIIQ
                     TLGTNVNLWRARFTELQGKGLIVEVGQRKCTVTGRSAIVWQYSGRTKPLELQHRAGTK
                     QLRSLLQRVVKACDVNGMQFAPSKTLITDIRAALQ"
     gene            28235..28771
                     /locus_tag="VMZ53_03755"
     CDS             28235..28771
                     /locus_tag="VMZ53_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27590.1"
                     /translation="MADNAPKKAPVGGGIRPPDYIRELALARVEDLMLQAYSPREIFA
                     KLHDEGFTESEATAKEWRLAIQKRWALEDAEQRPARKDAWRRRIESQYQKLLEHAETT
                     NSELARASYFAEATKLAKLALIMDGLTAPVKVEHSGAMMDPLALQPHERETRIAELLA
                     KREAALAAAARAGGKVVH"
     gene            28798..29376
                     /locus_tag="VMZ53_03760"
     CDS             28798..29376
                     /locus_tag="VMZ53_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27591.1"
                     /translation="MPIGAAYGAIRMQLRVCIDCDTCDQLHGAQLQGVLFKAVADGNL
                     APYTAIARAMIDDAQLTGRIVHDVKEGRVVDITIPNPPPRWMVAVGRAMARGEQPDVQ
                     TLVDAVLARYIGGQNTPALMAEMRIELRDALQFVDPSIMDVEVSATMTALDPGNLRIE
                     ITAKTPTLGSTMAGADDADIPPDIMSRPRAEA"
     gene            29381..30949
                     /locus_tag="VMZ53_03765"
     CDS             29381..30949
                     /locus_tag="VMZ53_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HUS27592.1"
                     /translation="MVAAAVPAWDEENEDELAALLAAQHEGEPLDAFIRRVSPRHAPP
                     RHISPLLDLWEATRYERVFACVELPPRHAKTTTGLHGIAWRLKRDPAVTNAFATFGDD
                     YAASRSRIARVLTRAGGVSLSKDMANLHEWRTNYGGGTICRGYQGEWTGQGITGVGLI
                     DDPFKDRASAESEKIRENVWEWFTDVWWTRLEPGSSCIVQHTRWHDDDLIGRLLAGKF
                     SGYRFQRIRLPAIAESVDGLGPDILGRQDGEALWPERYPVEELRKIEESIGPYGWASL
                     YQQRPRPKGADLFGEPARFSLLSWVPDGHRILICADCAATPHTRADYSALFVLAAKGF
                     GSDMTIDVLYGWRGHVSVPAFARKLLEVSRMFWGAPVVVEAVGGFKAVPQILKEIEPQ
                     LRVRVARMGDPNEGEKGAARVSNVPVDKFIRAQPAAAAWNRSRIRVPMDAEAVWNPQR
                     GRFEMPAVIMRLRAGVAPGVTWADELVHEAGKFSGVGDAEDDQVDALAHGFNELHSSR
                     NARRGADENRNPFG"
     gene            31023..31754
                     /locus_tag="VMZ53_03770"
     CDS             31023..31754
                     /locus_tag="VMZ53_03770"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HUS27593.1"
                     /translation="MSATVDKIRKLLNLAQNASANENEADTARRLAERLMAAAGISEA
                     DIADSATPDADPLATFGAQHVRSPEAGWHGILAVAVARVVGCYVHYSWQYEKGRRERA
                     LTWVGTQAQRESAIELHAWLISQVERLAKGAARIAKDHPAGSRAYLGAYRRGLASSIA
                     SQAMALGKNREATTQPDTNALARRSAIQNAIAAYEKQHDLRSTGSSYRTRALGAFAAG
                     VNDGRSVRLQHDMAGGSVKRLGAGK"
     gene            31751..31969
                     /locus_tag="VMZ53_03775"
     CDS             31751..31969
                     /locus_tag="VMZ53_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27594.1"
                     /translation="MRRPALTKAQRQHAREQRALLRLRRAAVRRSLVGDGEIEMADGD
                     AVTRADLDAACDAYTNVLTTRERRKLAK"
     gene            32188..32490
                     /locus_tag="VMZ53_03780"
     CDS             32188..32490
                     /locus_tag="VMZ53_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27595.1"
                     /translation="MAKKSNRETVTVGQPIRTRVVTSPSGVNGGSALAELFEREAAAA
                     IAKADADDAKLAAAAASAPFYTGSADPGTVVGLDGLAPSSETPKDGDSKRPDTDPS"
     gene            32549..34351
                     /locus_tag="VMZ53_03785"
     CDS             32549..34351
                     /locus_tag="VMZ53_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27596.1"
                     /translation="MRDRAPIGGGTVTVALAAPSAMTWSDFQQRHPEWRGDYWAECRA
                     LYAGGDRLLGDKAVLERLFPMHLHEVQKIYEERKKRSHYYPYAGTIIDHLLAGLGTDP
                     LRVSFALEDDQGKRELPPAAKWWERWVQDVTDTAERPSDYGLEDDDDGEDDDEGGTTM
                     HSFIVDVLREALQTRTAWVLADLPPLDPDAPAPIIDSKLAAEQLGKADPYLCVVPAEQ
                     VIDWQTSDDGKRLEWVIVMTQEQIRPSPKERRGLVRHTYVIWDAEVSTKYQIDIDPAN
                     LPAAEHPVPVASGPTPHGFGRVPIERLVLPEGLFAMGKLHSLAREHFNKRCAMSWAEY
                     KSLFAVLYEFLGPEDGTGMPVPMAQMDGNRAANQIRGQGYTQVRGKDDDARFVGPDVA
                     PFKEARESCNDAMREMHRVMFSMALSANMDSAALQRSGDSKAQDSATTAVLLDAFGQI
                     VRRFFRRMLVLAALGRGEAPPKCRVEGLEQFDVSGVADAISEAVELLNGVPILSPLFR
                     ELFLARLYTKVLGDVSHEQTAKIREQIRDAISAEETAIAGGLIPGVAPGGPAPGPKNK
                     PQLPEDAEPDEDEDAKDAKEKQPRGYSSKRKPKR"
     gene            34406..36049
                     /locus_tag="VMZ53_03790"
     CDS             34406..36049
                     /locus_tag="VMZ53_03790"
                     /inference="COORDINATES: protein motif:HMM:NF016148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HUS27597.1"
                     /translation="MQRVDAIAPEALRSLLPALRQARDELRADLSRWLATAPEGSERF
                     TAYHRAQALRSLEATFERIEDLHPAMAGALGMGRAETGALAVGNLEHEVQRLSSIFGG
                     GMVTLPQIDTAAVLAQGNRLLWKRHENSARRYAGSLGDDVRHRLAVSLAKGDTFDQMA
                     RRLRGSSEFAAMVARHDPGAAAFGVADATFARWRHWADRLVRTETMHAYNVQHDASIE
                     YVNEQRHEDDEEYLRRWDATADKVSCERCKALDRTVTTIKGDFAGGVHSPPLHPYCRC
                     VVLAWLARWGNIKGEVPALHKGGDEEIIPQEHIENERRAKASKEARAAAAEDLAAFDK
                     HEADKREAAANLQAAKERQAKQLAQHKEKQADKSREQVAPAEAVPVEFKQKKNPKRVA
                     AAKKAAEASAERRREIHSTVRDALSPELQVAWDKEGHKFMREQGKRIKGVKDRINASA
                     KLSEAFAETYGSGEQTIHGNEGDRSHKRAELVAQHAEHWADEQEHKHYEAERARAMRD
                     GLIDEHGNPTAKGKAQHKHEDLDDWGTPITKVDDDDPPF"
     gene            36198..36359
                     /locus_tag="VMZ53_03795"
     CDS             36198..36359
                     /locus_tag="VMZ53_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27598.1"
                     /translation="MLAARMLRKAAAAIPREAKQMLEAATRIESTGDELKAQLVSMLE
                     ASADPGTVR"
     gene            36417..37247
                     /locus_tag="VMZ53_03800"
     CDS             36417..37247
                     /locus_tag="VMZ53_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27599.1"
                     /translation="MGAKKKPGQQGNQAPQDDEDDDAGSGGGLTDEARTEINTLVHAA
                     INAQFSRKLPAAISSAMETVIAPLREQLANSNRGRAAEGDDDEGDDDQDQQPAARKKG
                     KQPAARKDPEVENMRKQLAKLTEEREQERTQARNRERDTMLREQLEAAGVDKNRVRGA
                     IAVLRDSMKYDDKAGEWIYSSKRDGYEEPLDVSTGVSEWAATDEGKSYLAPPSNAGQP
                     RGGSGMRPGAPNGGAPRVQNGGRPAQDPKAAKAQAKVDAVKSLSTAIDSLAGGVIPLG
                     "
     gene            37435..38466
                     /locus_tag="VMZ53_03805"
     CDS             37435..38466
                     /locus_tag="VMZ53_03805"
                     /inference="COORDINATES: protein motif:HMM:NF033394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HUS27600.1"
                     /translation="MTLIANSLTTIFEEQITSQINRATVLLQVLPVGPGTTGKNISWN
                     ARFGSAVGGARAEGAAVVTLNNDTKVPATLEFGTYDDAFGVTGKAMAAARAAKNPEEL
                     ANLFVDELGDCVERLAKGIAVDLYTGTGATDHIFGLVATGGGIMNTGTYAGIDRAVRT
                     QWQGTVMENGGVNRALTFDLMREMRRRIYSASGLKPDLIVTTPELHEKYGKLFGQQRR
                     YVQEIRLRGQTITLDGGYQMLEFDGIPVLEDVDCPAENMLFLNTRYVRVLQLPDQVDS
                     VNNSMSMIGLAGTPEEQLGQPSSKLSARINPLGRDGDSYKFQLICYPQLQVRRPNSCG
                     AIIDLDATL"
     gene            38610..39170
                     /locus_tag="VMZ53_03810"
     CDS             38610..39170
                     /locus_tag="VMZ53_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27601.1"
                     /translation="MQSEARVINVSPHVARFQIATTPGSQPRRYKLGPGESIHIQIGY
                     TQSFQGAGRQPVRATIESLTEREVWNKGPTLPMVVHESRAAEMRSRWQEMIARGAEPP
                     KPMDVYLPSADGGEPVKMTVQPAAAVTPAQVAAASSRLPAFDDDEDQSAAALDEPPPD
                     HNEPLEAVTVPAAPVPTKAESKGKGR"
     gene            39174..39575
                     /locus_tag="VMZ53_03815"
     CDS             39174..39575
                     /locus_tag="VMZ53_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27602.1"
                     /translation="MAVLTDEEKASIRMYMGWSARFAQTDTALERAMSAIDGMPADLA
                     LVRVHLTELARIDAAIQACEARFKADQVGSITLSQSELPRLRSRGMERVGRLATLLGV
                     EVRDNAFSPDLPRWRGGHGGPSGGGNYQMQG"
     gene            39688..40401
                     /locus_tag="VMZ53_03820"
     CDS             39688..40401
                     /locus_tag="VMZ53_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27603.1"
                     /translation="MLPMIITKDEATPAQRTVVFYAVDATDGFTPETAIDFSQAGDVK
                     LAKAGGALANAAGVVTELASGLYKLVLAAADVDTLGDLTVLITNAGVRPIVGHLQVVA
                     FDLNTATVDPSAAGITSIQAGLATSVEVAAIEGGTSSAFVAMGTRTTDGNFSAFSMLG
                     AKQTVITVTGTWDGATVTGQVCEDPSAAVPVWTAAGTPITANGTITLTGPHKAFRAAV
                     SNDGAGTSLTATAAVVTAL"
     gene            40412..40921
                     /locus_tag="VMZ53_03825"
     CDS             40412..40921
                     /locus_tag="VMZ53_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27604.1"
                     /translation="MAVPVLTGAATLNTSVLVDSLVPDVIDGLREDLHPAFGVRAYRV
                     YRVIRSWAGKTVGEGRHTDDAAELRPQPRVSVWSGLKYVQATCGIRELGDVQLSEVSL
                     TYTAEQLDPRTLRSNQELFYALGEANGQATPLRVWAHSAPPFIDREKTMGWLLFLRRV
                     EAAPAWSPS"
     gene            40906..41502
                     /locus_tag="VMZ53_03830"
     CDS             40906..41502
                     /locus_tag="VMZ53_03830"
                     /inference="COORDINATES: protein motif:HMM:NF016754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HUS27605.1"
                     /translation="MEPVVSNTIRVGLDQLPASLDLGERTIRRAISRGALAGAHRGRA
                     VMVRATPTDQGQLRASWKVKAGAEEFDGVGANLAELINDAPHISIVELGARPHAVSRE
                     GWEAIYDWVRRHFRSEGGVHVLGGGGRMVARSKDGRRDPRIEAITWGIVRKIKKYGQK
                     PTLFVRNNVDTLREVMAYELQRELQRAIANLTPKGGKP"
     gene            41499..42146
                     /locus_tag="VMZ53_03835"
     CDS             41499..42146
                     /locus_tag="VMZ53_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27606.1"
                     /translation="MSGVVRIEALRAFEALIEARVPELAGHVCTGVAPSSEHETVPNL
                     SIQPTKWTYEPNQAGEHTTLPGNVVVWDVGQHTCAAVLSIVAASPAQRWEIEAKVLDL
                     FLSSVHPLTGMHRPGVIVLPVSACPQLGAWFAAFELESDEWSDTLALDRRYESRIVIN
                     LVIPALVTEAPIYTINELILGVTEDMTTVFTPATAIPPAVELVSINEAGAITRYE"
     gene            42156..43691
                     /locus_tag="VMZ53_03840"
     CDS             42156..43691
                     /locus_tag="VMZ53_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27607.1"
                     /translation="MGDVYFTTNPAEFSRLPGLYVSERLPAGFIRGVDLGTVGMATKC
                     VRGPLTPVLITSSGRFTEVFGGRDYGSGGAAIGQGWLALVNKPFGTLVIRRVAAADAV
                     KASFTDETAAGGAGTAVLRVDASSVGAWGSNVKYKIGAATDGDANKFNLTVSYLGEQV
                     LYENLDIHAANYDNLAAAVGSDDARFVDLVKVASGRPKNTIAGADGADADGWIALGTV
                     AAGFTSVAGSEGTLAVGDYNSGMDDLAVYPGVGICLVPEAVTGSAVTFHSNLVTLASE
                     VSDRIFLTWAQAHGQSVSAEAAQVAAQITTRSDRIVWCYNSAYTLDPETDSEVQTAPH
                     VWLASILSQIDVDVHAGSFETVAYLAGITRVTNTSLSRPDLITLQDAGICQLERTTDG
                     FQFRSVVTTDLTEGKTELARRRACDYLQLSAADRLRYYVKRKNTATNRAGAAAELVAF
                     SEDLRARERVVEDYEIDQESVNNATQRARGEERILWRVRLIGHILSLVLETDIGTGTV
                     IER"
     gene            43739..44182
                     /locus_tag="VMZ53_03845"
     CDS             43739..44182
                     /locus_tag="VMZ53_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27608.1"
                     /translation="MSQKMRGQELTMRIAVDGVVQPGSMFKLTDFTATPRTEIVEDDY
                     LGEKETDLDIQHHGWDGAFNVDYEDAQAILLCEDILGRESNNEQHPRITITVIYTFRD
                     PTVRGKIAVYRDVFLKQDEESAGGRKEKVKGKFSFKCKSRVIMNA"
     gene            44248..44796
                     /locus_tag="VMZ53_03850"
     CDS             44248..44796
                     /locus_tag="VMZ53_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27609.1"
                     /translation="MNRAAPPAAAKPDMGDELRPDDVTDSPLARFSRELAQRYPQRMI
                     RRYEMPTMIRECREVFMVEITSRDEIEAAIMADATMTSLEKSSIRLSADAERRECIRL
                     SIVGLGKGSPVAYHHVNSDGEPFAEINEWSGKAWTLLQAYFGTLNGVPAEEIIEGLKG
                     AQTVGAFAPPTSATRASAESGK"
     gene            45003..46934
                     /locus_tag="VMZ53_03855"
     CDS             45003..46934
                     /locus_tag="VMZ53_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27610.1"
                     /translation="MRYSVDNRAGRTGTQGLTQDVRTLGNEAKRTSGVFGRLGLAVAG
                     AFGARAAGKALIGFNSTVQDTKNQIAGMLALSKKTDLSAQVAVADRLYGRLQTRAKSL
                     PGTTQEYVQMLGMLTQPLSTAGATLKEMEDLTVNAVVGAKALGISWDVAARDIDQALR
                     GQYHSVDQFTGKILGSMGYGGEEGRAKFNGMSAGKRKEVLQAALMQKQLTQLAAAQGA
                     SFSGMLSTLQDTVEQFLGKVGAPLFRVITNELKQWNGWIEANGDKIDAFASKLGEGLV
                     TGFRFVKDALSFLVDHADLLLMVGKVWAGVKIGGMLTSGVGKLGGAFGAGGKGAGFLN
                     FFKGARDGVDENGGYQYTPAGRGRSKVGGLKGAMGNLELLGGSAALGYALGNAMGLDE
                     VGKSVGAKLAFLTGRADGTTEAMDKLKLETDRLAEATRKATAANPGATGSIGMSQGVS
                     LDFKQQAALVEGVIKAEQKYKSEGGMDNMQWLAQHQMDMQAAGIDSSDFKKYGGAQGF
                     VDAMRGKATEIDARNDLSGTGVLIAMNLGVQKLTEYQRKTLETAQAQEELSRYMTVQL
                     SQGKLISPETVMEILRHNTQDPTGSHKSIADKPKVSVNIARIEVQSDDPDRMAFGLIE
                     AFRDAAKNPSSALSSLREG"
     gene            46938..48035
                     /locus_tag="VMZ53_03860"
     CDS             46938..48035
                     /locus_tag="VMZ53_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27611.1"
                     /translation="MARHTFVITELRSGTDPSTADEGERFEWTADRDPSKPFDGSKGG
                     GARACPMKPWPFGGQQRSSRTDYPGARSPSEQVLGPVRKPFTLSGKWDDRYNGDGYAL
                     AEMERFEAMVRRGNLCRFQYGPLALEGLVVDWDLEVNRLDFIRYSFQVSTHHKPDEKN
                     LERSPKTPSEPIALLDEFDTAVQAMLDADTSAPRALVKGTLADDITEQLVEITTARER
                     LATTIENRDSEAPEQPIDAFTRIATQFRSGRGAAYDLLLTLAEVRSDLDMAGGTAIAV
                     LEFEDWIRSLRFAARIAMGKGYDGDKAATERAEPNAVRLYRPRAGENLYAIARKFYGT
                     AHAWRLIYDRNDLRSFELAGTETLIIPERGA"
     gene            48038..49720
                     /locus_tag="VMZ53_03865"
     CDS             48038..49720
                     /locus_tag="VMZ53_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27612.1"
                     /translation="MTAGRVQLSSDGPRVFYPQCRAILQVIFDGYGASVRDSEILVIP
                     VLPKSVTVHINSYKQADSYEMVFDAGDLPIDPRLIRAGAAEIYLFQAPSADDRTRVIS
                     RRQPLVDADPGGLRRRDPVQTLALEMGTEGSRDKFTLGERKPRIAGPFDDTDIELSSS
                     GKWVTIKGQDYTAFLTAIQYPPNPNGTARRIPTGRRLDLIVDSLLEEADPDRQLSVDV
                     RGFNPAEMPIVGAKETRGNKRGIPVEQGTSYWDVIYKLVERHGFICYVDGLDVVISRP
                     KTITDRDTPNIKRMAWGKNLEHLSMRRHLGKEQAPTIVVKSYDERTGKTLTAEYPDGS
                     IDRAVIIDSTKGGKPKVHRRVKESTRRSKKTGKVKTTIRERDEYQIVQVNGITDPAAL
                     KRIAENRYHLLGKAERSVTATTRDLHDLRFADILNVSAGDAFWIEWDEFNRELLANPK
                     VSEEQKVRHLELRGFNSEIAREVAKRYELLAGLDRPLRFKEGTIEFDVEDGIKIEMEL
                     QDFMVVEGIRSDSGATRTPLLQQKREQQTKAGGKPIGWSEQYRKAQQRRWQR"
     gene            49717..50322
                     /locus_tag="VMZ53_03870"
     CDS             49717..50322
                     /locus_tag="VMZ53_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27613.1"
                     /translation="MRITKPKRGRGPVKRIDLTDMRELFRDRRMWSAVGIVTAPEGEP
                     YWEVVTNEDGSSVDVVVEVVLQPSEQHVTCRLRSGMWEVPAEGDEVAVLIPEGQIDFM
                     PIVIARLSGNAVPTTQGPSPTRIAIVAPSGGEVVVHDGSGGADPLVRRSEFLDHAHPT
                     AAAGAPSAPQRITGDPDAVPPIAPLAVGAVGFPGTDVLKAK"
     gene            50361..52460
                     /locus_tag="VMZ53_03875"
     CDS             50361..52460
                     /locus_tag="VMZ53_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27614.1"
                     /translation="MKATAIYALALMLALTGNALAGGTVITQPVGQSRVSGTCPPGSS
                     IRAVNADGSVVCETDDGSSFSALDVIPKGNGTGLAPSLLTDDTTMVCYNGCAFEADAD
                     GTTWTADIHSSDAFINNLIANGTGSFNGAVDIGGNLFVGGGPTKFTVQATTGNITTLG
                     NSILGNSDSDTTSIFGAGYSFGLTGTTLTGARNLRLYDTTATAANVGAAISLGYDAGG
                     GTGYQDPVIIKAVKENGTAANRDTALVFATATEAGVGTPTERMRIRSDGGMTFNGTTG
                     SDGYVLTVDPTTHIPKWAAASGATYSAATPLTLTGSTFDLAFTSDFQDSAGSLDLSTA
                     VTAPGSLTVATTLGVTGLSTLTGGVTTPASLTTTGTGDLVSGDDITSVDDVTVGDALA
                     VVGASDLRGAISNSTGTLTLSDSAVDFGADSSSVVTASNGIVHALNWYGGKHYEWNDE
                     YMDSSYSSFGFGALGWVTNPTGTGAAMVSYTGTAVSGRPGIAQMSTGTTTTGYSNAYT
                     SLGAGAAAGSMYVTEGTWAFEATGQFVNLSTSSEEYWSEIGFLDNPNAAGNGDGCFLL
                     YDRANTFSGVGGVNTGNADKWQACTNNGGTRSCVLLNGTSQNGSGGAGSITTVDTTLT
                     AASWWRAKIVAQTGSCAFSIDTGSGMTLRATMTINIPATATAIAMKIAKTVGTTARML
                     LIDQTAVSLDLTNVRSP"
     gene            52457..53107
                     /locus_tag="VMZ53_03880"
     CDS             52457..53107
                     /locus_tag="VMZ53_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27615.1"
                     /translation="MMRLFLLLALVACRLPHVEPALRSSHDQRQALAAITVSCTSPDP
                     FAGSPDVPNDPTPEAFPIEWNVPHQATGVVISDRHVLTAAHAVMCGAIPQADVQLANG
                     AHHVVVERDDRMFPHRSEETDVARLELFHAGRFDLGIRPPVIRDARGGEPCCAETLRG
                     ETCGRIDPYHLGYALGLDIRPGDSGAPLYCDGALVGLVTTDAGRFEPLTPYWMEGT"
     gene            complement(53110..53286)
                     /locus_tag="VMZ53_03885"
     CDS             complement(53110..53286)
                     /locus_tag="VMZ53_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27616.1"
                     /translation="MSDGNDGWVRLKINATPLPAQLHTWETDADGETWLVVYELRDGK
                     SVELRRTNARDVTL"
     gene            53318..53725
                     /locus_tag="VMZ53_03890"
     CDS             53318..53725
                     /locus_tag="VMZ53_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27617.1"
                     /translation="MRTLILVFALIASACVHPQAPAAPKLAPENQAVTYATKKVTPDA
                     ACLPLSTAQSGRADSAFCDLGRVLAYCTSGLGADGLCVALADMRPKAPEAPKTEPKPA
                     EQPAPPVPAAPPQQDAKPSTPSTPINMRRSGKR"
     gene            53722..54633
                     /locus_tag="VMZ53_03895"
     CDS             53722..54633
                     /locus_tag="VMZ53_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27618.1"
                     /translation="MKLNEAVAIARRIGPIRCVDSDGIPVTSGFGTAGAVEISVNDGA
                     FIAALGSITVIGGGYVYYNATPADASVRGFVSVKLEGICQESTFREEVERSPQGIPAG
                     TADAALCRVGPLRIVDADGVELASLAGITCEISINGSAWAAPSGLLVLTEAGYADYVA
                     ALSETASVGWLAVKLTGACQEFAMRATVVDATTGGGSLSAPTLTVLTTFSASFAAARL
                     TPWQATLDDFPVGAQAIILVHFVERNEMYVARDAEGVWRWPFDLEPDNAVDLNVDPVT
                     VQLIPRGGWHPCDIEVQIAAAVMAEEA"
     gene            54634..55134
                     /locus_tag="VMZ53_03900"
     CDS             54634..55134
                     /locus_tag="VMZ53_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27619.1"
                     /translation="MPGPSFTIPEPPSGYQQTPASEQATQDARFYGADIWLNVAAPDV
                     QLGQARYVTTAAGDWVLVRGREALRQSLMRRLITNPGDWQTKPDYGVGARQYVKAKNT
                     RTVRAELESRIRAQFSRDVRVHSVDLVTLTRLDGDKPGIKISVTVTPRGRLRTDQPLE
                     VSLEVT"
     gene            55137..56216
                     /locus_tag="VMZ53_03905"
     CDS             55137..56216
                     /locus_tag="VMZ53_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HUS27620.1"
                     /translation="MPVAPSFDDLIAQFEAEALAQNSELAFNDGDVTEAQAHGAGAMA
                     DAAIRFNVQAFKETFIDGAKGDALTALVDDHLNIQRQPATASQCTVRFSRTSGGAGFT
                     LAAGFVVASEFDDAGNTILYTLDADVTFAGADNGPHSGSVTAEITGKDGNVAAAKITR
                     FVDAKPDATIVVTNLAAAAGGNNEESDSELRVRARLFWQTLRRGTLAALEFGALQVAS
                     VRTVRATEDISTGAVTVVVADSDGASTAQMVSDAEAEIENWRAAGVSVAVLGGTQLLV
                     NVTGVLVVNDGVDAAALVPLVQAAIESRLAKQRQGELLYLDSVKAAGIGVDPDAIDAL
                     TLSLPAANVTPTATQTVRAGTITIT"
     gene            56227..>56606
                     /locus_tag="VMZ53_03910"
     CDS             56227..>56606
                     /locus_tag="VMZ53_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27621.1"
                     /translation="MALTADEQKFYDFAKASLPDWIPDGDEFLMGAAKMFGSVKALLD
                     YLFGQTLIGSAIGGTATTPDWLDQHARDRGTARQDGEDDPTLRDRLRNAPDALTRASI
                     LAAVNATLLANGITDPAGLVELPK"
BASE COUNT        10458 a        18300 c        19435 g         8413 t
ORIGIN      
        1 cgaggacgat ccaacgctgc gcgatcgcct gcgcaacgca cctgacgcgc tcacacgcgc
       61 gagcattctc gcagccgtca acgcgaccct actggccaac gggatcaccg atcctgcggg
      121 gctcgtggag ctaccaaagc acggcgcgca tagccgcacg tggacgctgc caaccggcac
      181 cggcggcacg ttcgcggtgg caggtaccac ggtgacattc acgccggcag tgctgccgtg
      241 gccgcgcaag ccgtatcgat cgccggtgct cgtgccgttc ggtcagatgc agctgttcct
      301 atcgggatgc gcgaacgcgg cgaacaacgg gctgcacgtg atcaccgggc tcgatggaaa
      361 cgctgccgtg tggaccgacg cgagcgcggt ggccggcatt gacgctagct gctcgtggca
      421 gctgcgcacg tacaacgcag agggattttt gcaggacggc gagggccgcg cgttctcgcg
      481 tggtggatac cgatcggcgc ggctcgtgcc gctgcggatc gtgatcatcc tgccatttgg
      541 cagcactgca ggaatcgagg cgagcgtccg cgaggcgctg cgcacgaaga aagcggccgg
      601 catcgccgtg accgttgaac gcagaacgat cgcaccgtaa agaaggagca cgaccatggc
      661 cacgctaatc gacacaaacg cacgtcgcgt gcagttcaac ccggacggcg aagggctcga
      721 cttcaccgac atgcagaaca tgcagacctt tgcgcaggcg cgaaactgga tcgctctgtt
      781 cacgcacgca ctggccaatg ctgctggcga tccgagcgcg ccgggcgatc tggacacccc
      841 ggaggcgttc gcggcgttca acgagttgtc gatgtacggc ggcgcggtga tcgcgttgca
      901 ccccggcacc gcgtgggtcg gcgcaggtgg cgctaaccag ctggcgatct ttccggggtc
      961 gatcgggctc gtaatggacg agccgttcaa caccggcgac gacttcgggc tattccgcgt
     1021 cacaggagtg ctcgcgctgg caacggcggt aggcgcagca cagccgcgga tcgatatcgt
     1081 cgaggtaaaa ttaactcaca tcgacggcga cccacaggat cgccatttcg aggacgcaac
     1141 gacacgtgag ccgtcgacgc agagcacgag caaagagaag cagctccaac tgacgtacca
     1201 gatcaaggcc ggcacaccgg cggcgaatcc cacgtatccg actccgagcg cggggttcac
     1261 ggcgatggcc gcggtgtatg tgccggcgtt gcataacgca gtgcactcgc cgacgaatat
     1321 tcgtgacctg cgtatgcccg ttggcgtgcg gtgtgtcgac gtcgacgcca aggcgatgtt
     1381 ctttaccggc gcaaaccctt gggccgtcgt cgacggcatg attgcgggcg ctgccgcagc
     1441 aagcgatccg gacgctgacc gtgtaatcgc gccgtgtccg atatcaacaa aatcagcgcg
     1501 actgcttgcg gttgccgtgc atgggttcgc tttcgatcct cgttgcgaaa tcggccggct
     1561 cgactaccca actgccggcg gcgcggccac gttcaccaag tacggcgatc tggtgaacgt
     1621 gagcggcgac atgctgcagg acaatatggt caccgtggac ggcatgagcc tcgctgacga
     1681 gatcgcggac gttgcagtaa ttaggggcgt gcgagctgct ggcgagcgtg tcggcacggc
     1741 actgtggtgt aacgggtacg cgggcggcat ggcgcacccg ggcgtgccga tgaacgacgg
     1801 cgccgaggcc acggtatcca agctcgcgat ctcgatcggc ggtgccaatg ggagccgcgt
     1861 aggcctcgtg cgcttctgga tcgcagaggg tctctgaggg atctgctcgg tgtttgcggg
     1921 ttgtatcgcg agccgatcgg atagagattt aatcatgccc gtcgactggc tggtcacgct
     1981 ctgggagaaa ggcggcgtcg ccctcgtcgt gatcgttctt ctgtttctgc tcgcgcggtt
     2041 cgtcggcaaa cgcggggtta ccgccttcga tctcatggcg aaagctcaga ccgactcgat
     2101 ggcaaagatc gcagacgcgg tgcagacgtc gacggctgcg aatgtggtcg cgcttggcgg
     2161 gctcggcgag cgcgtctcgc gcatggaggg cacggtggag ctgctcggca agctcgcgct
     2221 gcagcacggc gccgagccac aagcgcagcc gcaactgcgc acagtgcaga gaccgccgag
     2281 caagccgtag agatccccgc tcgccagcgg atgccgcact cgagcggcgc gggtgtagcc
     2341 tatgcgtgat gtctcgaatc gtcacggcgc tgatcgagcc gatgcaggcc aaggatctcc
     2401 tgccaatgcg tcggcacctg caggcgatca cggcgtcgca cgagcccggc acggctccac
     2461 ccgaccacga tcacccgatc accaaggcac tcgtcgatcg gttgccgaaa atgacgtacg
     2521 gcgagttgga ggcggcgcgc gagcgcctgc aaatgtccag cggcgacgac ggcggcaaaa
     2581 aatggatcag cagctcgcga gatcgcgatg gctggctgcg cctcgtcaat gccgagctgc
     2641 agcttcgcga tcacgagcgc gagcgtgccg gtgattcgtt gatcgatttt ctcaccaagg
     2701 gagtgcgcca atgaccgcga tcgcaggaaa gaccgactca cacgaaaact ctctgttgtt
     2761 gctgatcttc aacaacacga cgtacgccaa gatcggcgac gccaccggca tcgtcggcag
     2821 cacagtcccg ggctctctgt acgtgtcgct gcacaccgcc gatccgaccg aggagggcgt
     2881 gcagaacacg aacgagtgca cgtacacgag ctacctgcgc gtagccaagg ctcggtcagg
     2941 cgcaggctgg accgttaccg gcggatccgt ggtacctgcg tccgcgatca cgttcccggc
     3001 cggcactggc ggcgcgggaa cggccacgtt cttcggtgtc ggctgctcga caatcgcggg
     3061 cggcgccgct gatccgctgc tctacgcggg agcgatctct ccgtcgatcg tcaccggcaa
     3121 cggcatcacg ccgcagctca gcacggcaac cgcgatcacg gagtcgtgat ctaaaagccg
     3181 ccgcggcggg tgaaaggtcc gcgtgaccat ttcgatcacg aaccgcggga ccggcacgca
     3241 caacgtcagc gcgctgacgt tcacggtctc gcctgcgacc aactgcacga tcggtgcgtt
     3301 ggtcgctatt gctattgctg ccgacaacaa ctcgaccggc ggcgcgacga acgacatcac
     3361 gaacgttacc gactcgatcg gcaacacgtg gacggtcgcc ccgagcacag cggttgtgtt
     3421 cgacaacggc gcagccgcgg cgggcgtgca gggctcaatc tggactacga gccaaaacgt
     3481 cggtgcgttg caaacaggga ccgtcattac ggtcacgctc ggcaccgcaa ccaccgcaaa
     3541 gacggtcacg ctcaccgaat tcgccgggag caccggaaag gccacggtca ggacgagcgg
     3601 cgccaagtcc gcaggcgcaa cgagcactac cgcggcaggc aacgcgacca ccggcgcgag
     3661 cgcgagcgtt agtgtcggcg aggcgattct ggccgcggtc tacgtcgagg ccggcacaac
     3721 gatcacgctc accggcgaca gcgactccac taacggctca tggtcgacgg cgcagaccaa
     3781 caagattgga tcgacgacga gcggcagcgc gatcatttcg caggccaagg tccagacgac
     3841 cgcgaacagc acgcagtcgt atgacatcac gacgagcatc gcgagcgatt atcacgcgtc
     3901 gtggatcatc gtacaggaga ctccgtatgt cggatccagt gccggttcgt caactgccag
     3961 cgctgttggt tcgtcgacgg cggcggcgag tggatccgga tccggcggca gcacggcggc
     4021 agcagtcgga tctgcgacgg cggcatcggc tggatctagc gctggctcgt ccacggcgtc
     4081 gggctcgggc tcggcgatcg ttgtggcgtc cggatcatcc ggcggcattg gcgccgcgag
     4141 cggcgtcgga tcgtcgacgg cgcagtcaag cggatcctcc gctggggtcg cgacggcatc
     4201 ggccgttgga tcctcgtcgg cggtcggctc gggcacggcg gccggcgcgt cgacggcgag
     4261 cgccacaggc acatcgcttg taatcaccgc tggcgcatcc gccggcgcgt cctcggcaag
     4321 cgctgcaggc tcatcgacgg cgatcggctc cggatcttcg tccgggactt cgactgtcgc
     4381 ggccatcggg gcatcgacgg ccgcagctgc gggcgctggc gctggctcgt cgtcggcgag
     4441 cgcgatcggt gcatccactg cgcaatcctc gggatcgagc gcgggctcgt cgacggcatc
     4501 ggctgccggc ggggcgctgc tcatcgcgac tggatcgagc gatgggacgt cggcggcgct
     4561 ggccgttgga tcatcgacgg caacgagcgc agggacggcg gcaggatcgg ccacggcggc
     4621 agcgaccgga tcctcgtcgg cggaatcgac cggcgcgacc ggcggctcct cgagcgcgag
     4681 cgcgactggc ggctccaccg cggcggcgtc cggatcgatc gacggctcgt caacagcctc
     4741 tggcactgga tcgtccgtcg cgcagtcgac gggcgcggca gctggctcgt cgtcggcgct
     4801 ggcggttgcg gctggataca gcgtcggaat agctgcggga acatcgacgg ccagcgcgat
     4861 cggcgtcgcc atcgccgagg ccagcgggct cgcggccggc gcatcgacgg ccagcgcgat
     4921 cggcattgcg atcgccaatg cgatcggatc ttccgctggg cacacggcgg catcggcggt
     4981 gggcggctcg atcgcggtag caatcggcac cgccggcggg acatcgacag cggcggcagc
     5041 aacggacaac gagccgccgg tgccggcccc cgggccgagc ctcagcactg gcgtcgcggg
     5101 cacgatcgcg gcccagccag gaccaagcgg ctcgatcgcg atctcgcaat tcaccggcgc
     5161 gatcgcgacc cgaccgcagc ccagcagctc gctcgggatc tcgcgattct cgggcacggt
     5221 gacaacccgg cccggtccca gcggttcgcg gtaacatcgt gacgcgatga cgccgatcga
     5281 gctttggaag ggcgagagcc gtacgctgac ggtcacggtc gtgcatcctg acaccgggtt
     5341 tccggtggat ctctcggcgg gcacctggca gatcgacacg ctcgaattcc agatcaagag
     5401 cgacgtcgag gcggccgatc cggctctcgt ttccaagtcg ctcgtcggcg gcatcacgct
     5461 gctgacgcag tcgggcgaca cgctcggcca ggccgagatc gagatcgatc cggcggacac
     5521 gcagtcgatc acgccgggcg tgtacaagta cgacgtggta gccgtgttca cgaacgcgaa
     5581 acgtgttttt gtgatcaagc cgtcggactt gcagctgtac ggcgtagtga acggcctatg
     5641 atcgcggcag tgatcgcggt cagcttggcc gtggcggcga tcgcgggcgc gttcgtgtgg
     5701 ttcggcgtgg cactgcgaaa cgcgcagcag acggcagcag acgcacgcgt ggatgctgcg
     5761 aacaaggcag gcagactcgc gatcgcggcg gccgacgtcg cgacgcagaa gaatcgcgcg
     5821 gacgacgaaa aggagcgggc agatgcactc gatcaagagc tggaaaccgt ggctgctgac
     5881 ggcgacgctg ctggggctcg cgagcgcgtg ctgcggcgga aggcccgcgc cgcaagcgcc
     5941 gactccaccg ctcgtgacga ctccggggaa gtgcgcggcg gcagaccgga tcccgccgtg
     6001 gcgcgtagac ccgacgacga tctcgccaag cctggtgatt agcgacgttg agcacctctg
     6061 gcagcggatc gacgatctcg agctgttcgc cgatcgggtt cgcgcgagct gcggccggcg
     6121 agatcccgcc cccgccgggc cagcgagcag tactcgaggg ccgtgatagc gtggcggcat
     6181 gtcgcgccgt gcattcgtct ggatagcctt tgccgtctcg ctcctgttcg ctcctctcgc
     6241 gtggtcggat accccgagcg atgcgggcgt cgctgccgtg tccgctggat ctgcgctcga
     6301 gggcagcggc agcgctgcag gtgtcacgca gcccgacgtc gtgaacccgg cgcagcatcc
     6361 tgcgcaggcg ctcgacgagg tcaagctggc tcgtaagacg agctggccgc ttgccgtgtg
     6421 gctcgcgctg gccatggcgg gcaaggcgct cgcgtatgga cgcgacaagc tcaagcgcgt
     6481 gcccgttgtc ggcggcgtag cagcgctgct cgcaaagggt aagggcgcga tgattgtggc
     6541 tgccgtgggc gccgtgggcg ccgctggata tgcagtgctg ctcgacggcg gcacgctgac
     6601 ggcggcgctc gtggcgagcg ttgtggcgct cgctggcgtt acgcgatcga cgacgaagac
     6661 aaccggcggc gaccccgccc cgtcttcgcc gagttagctc agctggtaga gcgacgtttt
     6721 cgtaaatcgt gggtcgccgg ttcgattccg gcactcggct ccaagcgccc cgcattgtcg
     6781 gggcgtttct cttttcacgg cgcagtgaca gcgcgcagag cgtgcgcctt gtgcccgagc
     6841 ttgctccacg agtggttatg cacatccatc gcgtatgcct tgttacgcgt tacggccggg
     6901 tgtctgccgt tggggccatg agagtgcaga acccacgcgt ccgagtaggg gccaccggaa
     6961 agcgccccct tcggatcgtc gatcatctgc acgtggccga tccactcgtg gcgctcaccg
     7021 tcgacgacga gccggattgt cggatacgcg agcacgtcgc caccctcgag tggatcgccg
     7081 aacggtacga acgtgaacag ctcgccgcgc accacggcgt ccttgatcat cgagttggtg
     7141 tttatatcgt cgacgacatc ggcgagcgat gcccacgcag gcggcagcgg gccgcgcgca
     7201 aacccgggcc ggtgccgctt aaccttgaac gcctcgcaga tcgcgccagc gcagtcccat
     7261 gggccgcgtg gtggctcgcc gctgaagtta cccgtcccga gaatgtatgg gaaatcgagc
     7321 ccgaccatgg agcgcatgcg cgcgagagcc tctgcaggtt tcagcatggc gtcgacggta
     7381 gcgcacagct ctgtcatagc gatgcggtaa ggctcgaggc gagatctcca cgccgcgcaa
     7441 cgtttaccgc agagctgtgg agcctgttac aaggagcaac cggaggatcc aatggccaac
     7501 aatacgaatc cagatcatga aaagttcgat tttgcggagt tcttcgccga gttcggcaag
     7561 ggcgccacga accgcgtagc gtcgcaacgc atgcaagaga tcgtcaaggc gtgcagcgag
     7621 acgggccgca agggcaagat cgtgattacg ctcgaggtgg gcgcggtcgg cggcgttgcc
     7681 gagctgcgcg ccaagatcag cgttaccaag cccgagccgc agctgccggg tggtgtctac
     7741 ttcgcgaccg aggacggcgg gctcgtcgag gaagatccgc ggcagctcaa gctgcccgtg
     7801 gcgcgtgtga tcgatctgca acccgtcaag aacatcaaca aggagcaagg acagtgatcg
     7861 aagacgtttc tcagatcgtg acaaccgccc gcgagggcat ggacgtaaac acccggatca
     7921 ccgagtgggg gcatcccgac gcggccgacg acgacagcgg gccggcggtg ccggtcgtgc
     7981 tcgtcaccga ggtggacaac gatggcaatg cggcaacgcg cgtgacgctc gcgggcgata
     8041 tcctggccga gctggatcgc cgcatgcccg gcccgcgtcg acgcgagggc cgcgtgcagc
     8101 tgcaggacgt gcaatcgttc tgcgagtacc tctcgcgcta tcagcgcccg gagagcagtg
     8161 tggtttacgc gaacgtctcg gcgatgacgt tcgaggccgt gatcgacgag cacccgcttg
     8221 ggctgccgaa tgacgagatc gcaaccgcct ggcgtggcca ccgcgcgatc tacgcgtgcc
     8281 cgcgctcgcc cgagtggctc gcgtggacgg cgaaggatgg cgccgcgatg aagcaggacg
     8341 aattcgcgga tttcctcgag acccgcctcg aggacatggt gaccggcgaa ggattcccca
     8401 agccggtaga ggtgctcgcg gtggcgcgac agctccacat ccgcaccaag ggcacgttcc
     8461 agcgcgagat caacccgacg aacggcgaca acatcctcgt gcacaagacc gagaccgaga
     8521 cgggatcgac gcagatcccg cgcgcgttcg ctgtagcgat cccggtgttc gagggaggcg
     8581 agcgctatca ggtcgaggcg cgcgtccggt tctcgatcgg cgatcgtggc ccggtgttct
     8641 cgtacacgct ccaccgtcga tccgagatcg agcgcgatgc gttcggcggc gtgcgtaaga
     8701 tgatcgcggg caccaccaag ctgccggtcc tctcgggcat cccgggctga ctgtcagacc
     8761 ctctcgttac cttgccgcta caaaccgatc gagggtgccg catggacgcc tcggtggctt
     8821 ggtagctccg ttgctcctcg tcaccctcga tctctcaaca ggagcggatc gacatggtgg
     8881 caacggcagg acaaagcaat ctcagccccg agcagctcgc cgagcgcgcg cagggcatca
     8941 cggcaaccga cgtggcggcg atcgtcggcg ttcacccgta ccgcagcgcg gtggacgtgt
     9001 ggagcgacaa gctcgggctc tcggctccgt tcgtcggcaa cgatcggacg aagtggggaa
     9061 acatcctcga gccggtgatc cgcagcgact acgaggagcg caagggcgtg cgcgtcgaga
     9121 cgcgcggcac gctcacgcac cacgagcgca cgtggatgaa agcgacgccc gacgggctcg
     9181 tgtacgtggg cagcgacgcc gagccggatc gcggtctcga gatcaagtgc catacgatgc
     9241 acctcgcgta tctctacggc tcgccgggca gtgacgaggt gccgcaatac gagctttgtc
     9301 aggtcggttg gaacatggcg gtcaccgagc tacagcggtg ggatctggtc gcgttcatcg
     9361 acgggcagcc caccgactac gtgatcgatc gcgatgacga gctgatcgac atgctgcgcg
     9421 agcgcgccga gcggttccgg cacgactacg tgctcgcgaa gacgcccccg ccccctgacg
     9481 gcagcgaggc ttatacggcg tggttgaacg cgcagcacgc cagcgacaac gcgttggcgg
     9541 cgctcgtcga cgtgagcggc gacgtcgaga ccatgcgcaa cgtggagcgg ctgcggacgc
     9601 tgcgcgacga gatcgtggcg ctcgaggcgc aggagggcct cgtggtgcag tctctcaagg
     9661 cgcggatcgg tgaccacgcc ggcatcgcgt ggccgaacgg caaaccagcg cccaagaagg
     9721 gcaagctcgc gggccagctg cccgtcgata aaatcacgtg gaagaagtcg aaagacggcg
     9781 tgcgcaccga ctacgcggcg gcattccatg cgctcaaggc tcgcgcgcag ctcgtcgcca
     9841 gcgcgaacgc ggacgcggcg catcgtgcga tcacggcgct cgacaagctc gccgatcagt
     9901 atttcgcgaa cacccgcgca acgacgacgg gcaaagagat ccgcgcgacc ctcgagcagc
     9961 tgcgcgatct ggccgttgaa gtggctcacg ccgagcccgc gacggcgccc gctccgggat
    10021 cgcggcggtt cctcgtgccg aagcactgga agggcaccgc agacagcgcg gacgagtaga
    10081 aacgtagtac aacagtaaac cccataaagg agcacagaca tggaaactca cgacgacagc
    10141 acggaacacg aatacgacag cgcaccgcgc ggcaatcagc tcgcccggcg cgatttctcg
    10201 ggcagctcgc tctcggtcgc cagtgcggca accgatgcgc tcgtggccaa ggagcgcgcg
    10261 aacgtcgagg cgcgctggat tatggcgatg cgtcgcccgc gcaacatgga tgacgtgcgt
    10321 cagctcatgc tcaaggagtg ccgccggccc ggattcgctg acgcagccgt ctacgagatc
    10381 ccgcgcggcg gcaccaagat caccgggctt tcgatccggt tcgccgaagt ggccgtccgt
    10441 tgcatgggca acctgcagcc cgaagtggtg acgatccacg acgacgacga cacgcggatc
    10501 gtgaagtgct cgatcacgga ctacgagagc aacatcacgt ggagccgcga catcagcatc
    10561 cgcaagacgg tggagcgcaa gcagctcgcg cgcggccagc gtccgctcgg cgagcgcacg
    10621 aacagctacg gcgatcgcgt gttcatcgta ccaggcacgg acgacgacgt ggccaccaag
    10681 gaggctgcga tcgtgagtaa gacgctgcgc acgctgatcc tgcggtgcat ccctggccac
    10741 ctacaggacg agtgcctggc gctctgcaac aagatctatg cagacaaggc cgcgaaggat
    10801 ccggacgccg agcgcgtcaa ggtgctcgat gcgttcgcgg gcattggcgt gctgccgtcc
    10861 gagatcgagg agctgatcgg ccacaccacg gagcgcatgt cgccggccga ggtcgatcgg
    10921 ctgcgcaaac tgttctcggc gatcaacgag ggccacgcat cgatcgccga ggcaatcgac
    10981 gcagcccgcg ccgatcgcgc gaaggctccc aaggcggcac cggcgcagac tgcaacccca
    11041 ggagacgaag gcggcaaaca gcctacgagc gcgcaggctg ccccacaagc gacgaacgca
    11101 gcaccggcgc agcaaccggc cacggcgcaa ccggcggcgc cagcgagcgc aggacaggcc
    11161 aaggcgagca agagcggcaa gggtaccgcc gggctcaaaa acgcgctggg aggcaacccg
    11221 gacagctcgt tcgcgcaggc tgccgagcag gctcgcgcca aggatgccgc gcgcgaggcc
    11281 aaggcggatc ccgaggccga gcccggatgg atgagcggca aggccgacga cgccagcgag
    11341 gagcgaccct gcgcaggctg cggcgtgccg attgacgcga agaaatcgga cccgcccggc
    11401 ggcaagtgct acgcgtgcct taacgcctag cctgtttact tccagaaggt aaccggctaa
    11461 acccgcggtc tcggatgggg atcgcgggtt tttacgtttc ggggttgcac gcggtttata
    11521 gcactgctat aaattcagac atggcaacgc aaacactcta cgcgattcgc aacaacctga
    11581 ccggcacgat cctgaccggc gcggacggca agatcgcgac gtgggcggat cgctcgatgg
    11641 cgtctctcag ggcaagggag tacgggctcg ggttcggctc tttcgcgatc gagacggagc
    11701 ccgcaccgac ggactcggat ctcgtgaccg tgcgcgaggc gcagcccgag gagccgggcg
    11761 agggtatccc gcctgcgccg tggctcgagg aagggcgcgg cgtgctacgc accgcggtgg
    11821 ccgatcgctg cgcgagctgc aagctgccgg cgcacctcac cgacgtgccg aacaccaacg
    11881 agcagcaccc ggcgctcggg ctcgcgtgct ttgcgtgccg cgacacgcac gacacgggcg
    11941 ccaagctgct cgcgatgctc tcggcggcgc cgagctgtca gctctcgcgc cccacgatgg
    12001 cccgcaagct cgggcgcacg gcgagcagca tcgatcgggc tctcgcctac ctgcagcgcg
    12061 agcggctgat cgagcgcgca tacaagagcc cgaacatcga gaagccgtca gcgaccacct
    12121 acaagctcac cgacgcgggc agccgcgcgc acttcgccaa caccgagcac aaggagcaac
    12181 atgcacacgc ctgagagccc gttcccgttc accaccaaga gcggcgccgt cattgttgac
    12241 gagcgctgca gctgcggcgc gttgcgcagc caacacgaga ccacgatcgc gtacggccac
    12301 gggccgcgcg ttgtggccgg tgccgtcacc tgcagccgct acacgttcgt gcggtgggtg
    12361 ttcacgtctc gggcgctgcc ggccgggttc gaggcgctcg acgacaagca cgccgtccgg
    12421 atcgagcggg cgagcaagcg cgacgtcggg gcgagcggct ggcgctacga ggtgcgcgac
    12481 atgagcgcca agctcggcgg cgccaagctg ttcacgtgca aggcgcccaa ggcgcgcacg
    12541 agcctcgatg cactgcgcca cgcccgcgag gagctgcgga tccacctcga gcggcagccc
    12601 gagcttggag gcatctcgtg atcactcgtg acagggtgga aacgaacggc atcgcgaaca
    12661 acgcacggca gcggctcgga gaggtgatcc ggcactaccg cgaggatcgc gacatcacca
    12721 agcgcgaggt gtcgaggcgc tgcaaggtgc ccgaggggct cgtcgatcgc tgggaaggcg
    12781 gcgaggcggt gcccaactcg gatgaatgga aagcgctctg ccgcggcatg aaccgcgcgc
    12841 tcaacgccta ccaggagctt tacctgcgcg ctcgcaagga agcagacgac gacgcccgct
    12901 cgagggcggt gcagaccatg agcaacgaga aaccaaagga gcccacgaag atgcagaccc
    12961 cgaccccgaa caatccgtcc gcgaggatct ccacgagcct cggcgacaag ctcgccgcgg
    13021 ccaccgcgcc caagccggcg atctcgatcg tccgcgatcc ggacccggag cccgcgcccc
    13081 cgagtgatac tacgtcgagg acaacgcgcg cagctatggc caagctcccc aagggatgga
    13141 agtcgcacga gatgctcgcc aagcggcgcg cgttcgcgct cgatatcctg cgccagcgtc
    13201 cgaatatccg cgacagcggc gcagactcgc tcgaggaatt gctgcagcgc gcgttcggtg
    13261 ttgggctcgc caactacacg gtcaaggagc tgcgcgaaca ggccaagcgc gaggccgatg
    13321 ggcgcccgat gccggtagca agcccccctc cgatgcagtg gcacccgccg atcgtccaac
    13381 cgcaggcgcc tgtgacccac gccgaggccg tcgaggcgca acgcaaggcg acggcagcga
    13441 tcgcgagccc cgaggatatc gcgcgcatgc acggcgccaa gcctgcgccg gtctccacga
    13501 cgccgacgag cgcgggcgac atcgaaacgg cggtgcagct cgtgctcgag gcgctgccga
    13561 acctgcagac gttcacgatc cacgtggacg agcacggcga ggcgtccgtc gactaccaga
    13621 tccgcaaggt cgtgatcacc accgacggcg gctcgttcaa ggtgaagcgc tagccatggc
    13681 gcagcggctc aaggcgaagg ccgaggcgat cacggtgcgc aaggcgctcg ggcgcagtcc
    13741 gcttgctcgc ccggcgttca ccgagcccgc gccacctctc gagctgacga tcgagacgac
    13801 ggacgcgcag cggctcgccg atgccgaggt ggctctcgag cagccgaaga tcgcacgcca
    13861 catgctcgag gatgctcgca cccggctggc cgcggccggt gcgggcgggc cgcacccggc
    13921 agccatcgcg gtagctcgct ctcttgccga gacgtacaag gcgatcgtgg gcgctcggct
    13981 cgtggtccga accgtcaagc gataggcgag tttacgcgat tgttcgaggt gccccattgc
    14041 gcatagttta tagcaccgct ataaacagga catgacgaac aacatcacga tccacggcat
    14101 cctcgctggc gcgtatcgcg gtaagggcct cgagcgtacg ctcctcacgc acgcatcggc
    14161 ggacggcggc gttacggcgc tctgccgtac catcaaggcg gattcgctct gcgacgaggt
    14221 gctcgacgag gccccgagct gcgcacgttg cgagcgcaag gtagctgacc gccgcaagct
    14281 ccctcggttc aacgggccgc agtcgtacac ctcgcatcaa cgttattggc actgccgcgt
    14341 gctcgaggcg caccgcgatg tctacatcac gcccggcatc gggtggtgtg gcccgggttg
    14401 gcagatcgtt gacgcgttga tggctcgcat cgcggccggt gagtcgttcg gcccggagtc
    14461 atgggcaacg gcactcccct cgcttgtcgc ggcggtggcg tcgtgatcgc gttcttcctt
    14521 gtagtcgcgc tggtcgccat ggctgccgcg ttcgcgttgt cgtatcgcgg ccagttcgat
    14581 cggcatcgcc ccgggtgccg cacgtggcgc ctgttccaca cgggcgtgca cacggagtac
    14641 gcgggcacag gccgggtgcg gttcgtgtcg ttctttcgcg gtgccggcta cgtcgtcgtg
    14701 ctgcgcagtg ggcgaggtcg cgacggtcgc ccgctcgccg agcctggctg gcgcccaaac
    14761 atcgagatcc caggcttcac cattcttgtg cgcaccgcga agtcatcgcg ccgagcgtcg
    14821 tacggcacac cggtgcgtca cgtccgcatc acacgcaacc cgaatcaacc cgcctgctaa
    14881 ggagccacat gatcaccatg gaacgaatca ccgagatcct gcacgaaggg ctcaccaaca
    14941 cgctgcgggt gcacgtgcct gcgccgagcc cgatcatcga tcgcatcgcg cgcgacgcgt
    15001 ccaacaatat cgcgatggcg ctcgcgtcag agatcggcga gacgctcgac gaagcgcacc
    15061 tgcagcagat cgaacacgag agcctccgca ccaagctgca caacgcgggc gggcacggcg
    15121 ccacgctcga cgtgatgctg acggcggtac tcgagcgcgc ccggctctcg ctcgctgcac
    15181 gcgacgcagc tcagcagctc gtcgacgcgg catcggagct gctcgaggcg ttcgacgagg
    15241 gcgacgtcga cgtcttccat cccgacgatg acgatccggt tctgccgaac gcaacgatcg
    15301 agtgtcccga agatgacacc tgcaagtgtc gcgggccggc tgcgctcaac gagctgtctc
    15361 gagcctgcgc gaccgcccgg cgcgagctgc cgcacgtgga gcgtgtgcag tgatcccgcc
    15421 gagcaaacgg atctggatct accccgatcg cacgttcacc cgtccgcgcg atctcgagat
    15481 catcgtgctc ggcgagcccg aggtgatcgc gccgttcatg gtggagctgc gcaacacgat
    15541 cgagagctgg ccggggttcg atcaggttca cccgatcacg atgggcgaac agctcctcgg
    15601 cttccgcgcc tcgcgctcca agctcgccga gcacgacgac ccggtggccg aggccatgct
    15661 catgctcaag cggttcccga tcgagtggac gggcaacggc tatggcctcg ggatcgtggg
    15721 gctctggtaa tcgtccgagc gatctcgcgc gttgaccgat atcataccgg ggctgtaaac
    15781 catcgaggac gtaccatcat gcgagcacta ccactcacca acaaaagccg gcccggcgga
    15841 cgcccgcgcc aaacgcgcgc accgcgcaac ccgttcgccg actggctcgc gagctgcggc
    15901 aagacacccg agcagctcgc caaggcgctc ggcgtcagcg tctctagcgt ctacaacgcg
    15961 cgcaacggct acttcaagcc cggccgcgat ctggccgtca agatcgccga ggtcagcgag
    16021 ggcgccgtcc ccgtcgaggc atggggctcg gtgcgcgctc gcaagcgcaa ggcggcgtag
    16081 tgttcgcacg gttcacggag gaaccgcgca tggcgtttgg tgacacgcga gggccggtca
    16141 ccgaggtgcc gtcgtacgtc gtgaaggctg ccggcgggat gggctcaccg tcgaaggcgc
    16201 accgcccggt ggcgcgcgtg gaacgccgcg gagcgttcgt gcgcgtgcgc gtgtggttcg
    16261 cgagcgaggg cgctgccgtg tggttctcgc ccggctactg caccctctcg ctgccgcggt
    16321 tcgcgctcct caaggcccga tcgtggtggc ggcgatggcg cgagcccgtg ccgcgcgctc
    16381 acgtggtgca gcgatgacag atccgggcga cgaaaacgac caccccgaga ccgatgcgga
    16441 gtggatcgcg tggctgaagc gagagctggc gcgcgcggcc gatcgtggat cgcgtggcca
    16501 gacgctcacc ccggcgcagc tcataggagc gcagcgcggc gtgctcgcat acccgatcac
    16561 cggcggcgag aaccaagatc cgggcgacga gagcgacgcg ccgatccgtg tgtggctacg
    16621 caagctgact gtcacggtag agctgtgagc cggttccgct acaaccggat cggcgtgccg
    16681 tcgatcgcgc gcccgcccga gcggtatatc ggcgagctgc tcgaggacta cctcgcgaaa
    16741 tggcaggccg tgcgggaggc tcgtgtagtg aaatgcctgg ccgacatgct cgggcacgcc
    16801 cgcgagcaca cggcgatcgt tgaggtgcca ccgcgccacg ccatgagccg ctcgctctgc
    16861 gcatactacg gcatcgatcg caccgcacac ccggagtcgc tcgccggctg gcgtagctcg
    16921 aaccaggagc cgttctcgat cgcgacgttt cgcgaggcca tggtccgctt gctggcctac
    16981 gagccgcccc cgcctgcgaa gccgtggatc acgcgccagc gggccgcgca gctcgaggcc
    17041 gagggcgtgc ggcgctctct gatcgaggct gtgttcgaga tctactgacg tccgggagcg
    17101 cgcctttgcg ctacaccgcg cgtgcgcgcg cacttttttg ggcctgtgaa gatctccggg
    17161 ttatccacaa ccgcggacgc gcgcatcgtc gacggtgacc gagaaagtta cccgcagtaa
    17221 ttcacagctg tggagtaatt gagggcgagc gctggcgcgc acttgtgccc ttgcgtgagg
    17281 ctctcgcgtt ttgctacgta ggcgttgacg cgcggcactg gatctcgtgg tgagggaccg
    17341 agccggcgcg attgggagag gtgcgtccga tgtctgaagt agctccgatc gccgggccga
    17401 tgaccctggc tgtaaccgtg gaggctgtag tgccgctcga ggtgttcatg atccgctacg
    17461 cgctggcggt gctcgccgcg cacgacggca acaagacgca cgcagccaag gcgctcggcg
    17521 tgtcgcggtt catgctgcag cgcctctgca agcgagcggc aaccctcggc atggcggcac
    17581 ggtaatgcgc gtcaacgtcg acgaacaggc gatcaacgag ccgcggatca agcgcatggc
    17641 tcggcgtctc tccaagcgac acgatcgggg gatccaccac accgaggttc taggccggct
    17701 gatcggcgtc tggatgctct gctactcgcg gcgctccccg gtgctgcgcg ctgacgacgt
    17761 cgacatcgcg gcggacctgg atggattcgc cgaggcgatg atcaccgacg agatggcgga
    17821 caccacgcac gccgaggaca tgatccacgt gcgcggcgtg tccgaccgga tcgcattcct
    17881 cgagcgacag accgagcgag gccgcaaggg tggacgcgtg gcagggcgag gcccggagca
    17941 gcaggcgcaa gcccgcttat ttgcagaaga tgaagcgttg gctaagcgtg tgcttgagcg
    18001 aacgcaagcg aacgctaagc ggcatcaaga gcgtacgcta agcgaacgct tgagatcgcc
    18061 acaagcctac tctccggatc aggctccgga tcaggctctg gctctggatc aggatcacgt
    18121 ccccactcgt ggggccggcg acgtgcagca acgccaagcc ggtgaccccg aaaagggctc
    18181 tggcttgccc gtggcgagcg cgaaaccatc cgacccgacc gagaccgcac ccgagaccga
    18241 gaatcgcgcc acgggcaaac caccgcgaga ggtgccggtg gaagccgtaa ccctggccaa
    18301 cgtgctcttg gggcagatct gctcgaacca tcccagcggc cgagcagcca agatgccgcc
    18361 caaggtcgcc gaggtcaccg cgctgcggtg ggccgagacg atcgacaagc tccaccggat
    18421 cgataaattc tcgtggggcg agatcgaggg catgatcacg tggagtcagc gtcactcgtt
    18481 ttggcgcacc gtgatccttg gcgccgacaa cctgcgcgac aagtgggatc agatggccgc
    18541 gcagcgcaac cgcggcgcca cgccgcgtga tacgcagccg gcccctaacg tgttcgacgt
    18601 tctcgacgac tacgccaaag aaaaagacgc caaggacaag gagccaaccg agtgacaaac
    18661 accgagatca cgcaggcgct gaaactgatt ttcgcggcat acccgcagca acgcgcgcgc
    18721 atgtctgccg acgacgtcaa ggcaatgcac gacgtgtgga gcctggcatt cgccgacgtc
    18781 tcactcgagc cggcgaaggc ggcgatcgcg cgccacgtgt gcacgagcaa gtggatgccg
    18841 tcgatcgccg agatccgcga gcagctcggc acgctgcacc acgggctcaa gcgcagcggg
    18901 atcaacgcgt ggggcgatct caaggcgctg cgcacgtacc gcgagcgcga ggcgatggag
    18961 caagtcgatc ccgtcgtgct gttcgtgtgc cgcgcattcg actggatcga atggcgtacg
    19021 ctcctgaagg ccagcggcga tatcgagcag tggcacgtcg tggtgccgct cgagaacgag
    19081 gccagcgacc gcgcgcggtt cgcggagctg tacgacaaga cgatcgcgga cgagcgcaca
    19141 acagcgcagc tctcggcggg cggcaagatc ccggcgcagc tacagcgatc ccgtgacggg
    19201 cgacagctcg gtgatatcgt cagcggtcta ctccccgaga aaacatgatc acggtggcac
    19261 tactcgaggc gctgatcgcg ctgcacgtgc gcaagcgccc accgatgcgg cgcgtgctcg
    19321 cgttctggcg ctggcgtacc gagcttatgc ggctcgagga cgcacgcgcc gaggcgcggc
    19381 tcgcgatggc gttgcaggtc tcgaatggat gacgtgatct cgctcgtcat gagcacgctc
    19441 gtgctgctct cgagcatggt cacgcagccg cagcgcgcga gctgcccacg ctcgtgggtg
    19501 atcggcactg gcgtgcgcgg tggcgtgttc atgtgtcagc cggatcgcat cgggtgcgac
    19561 tacctcgatc gcgtctgcga acagccgccc gggttcgtga tcggtcgtgt ctactgcggg
    19621 ccgggcctcg agcccgcgct cacgcgctac gccgatggcg tgcgctgcca acgccagccg
    19681 aggacgtaac gatggccgct agcactgttc ccgatgccgt tgatctgcac ttgctcgaga
    19741 gagcctgcgc agagcgagac gccgcgctcg agatgctcga tcgaatggaa cgagctttcc
    19801 cgggcggaca ctcgagccac gaggtgcaga gcatgctgcg cgaggcgcgc gcgatgctcg
    19861 tcgattgcgg cgttcgcaag agcgacgccc ctccggtctg gcgggatcgg cgttgattgt
    19921 tgtcatagca gcgcggtaaa cgtgggcgat gctgaatctc tccgataaga aagttacccg
    19981 cgcgcagctc gtcaccgaga tcgaggagct gcgcacccgc gagagcttgg cgcgcgaggc
    20041 gcgccagcat ctgcagaatg ctatcgacga ggcggacgcg aagatcgcca acctcgagca
    20101 caagtgcgcg gtgctcgaca cggacgtgca caacatgctc ggcacgatcc gcgtgatggc
    20161 cgatgcgtcg cgcgccgtcc gcatccgcgc caaatcggtg cagtgcgccg atctcgcgat
    20221 agcgctgccg atgcgcaaca cggacacgct ctaaccgcga tggcgcagct ctctctcggc
    20281 ggtctcggcg gcatcgtgct cgcgcccgac gtgctcgagg agcagcgcgg ggcgtggtgc
    20341 tcgccaccgg agtacacggc agcagcgacc gagcgcgggc cgttcgacgt cgatccgttc
    20401 acgaatccgc gcagcacgct cgaggcgcaa tacaagtgca tgcttgagcg cggggatgac
    20461 gggttcgggc tcgatcgccg agacgtgccg ggcacgttct acgtgaagcc gagcggcatc
    20521 gtgcaggccg attgcttgaa gtgctacggc ggtgggcgcg tgctcggcaa cgatctggag
    20581 ccgaccgcgt gcccggcgtg caacaacgga taccacgtcg ccaccgagcg gacgcgcgtc
    20641 tggattcagc cgccatatga cctcgtgctc gaggcgctcg cgcactacgg gcacacgcgc
    20701 tttgtggcgt tgctgcggct cgacacgtcg acggagtggt tcgagcagct ctggaatctc
    20761 tcggaggtga tcatggtgcc caagcgcgat cggctcgagt tcgtgccgcc gccgggtgtg
    20821 aaagcctcga gtaacccgtt cccgcatggg ctttactaca agcgcgccga ggatgtaccg
    20881 aacgcgatcc gggcgctctg ctacccgtgg ccgtgcccgg cgtacccatg ggcgagcgat
    20941 ccgctcgggc tgcgcggcag tgcggcgcat cagccggtgc ggtggcaggc atatacgcac
    21001 gtgccggcga cagcgaagga gcgcccgaca accgccctac acgttgcagc ggttcacggc
    21061 ggcgcggtgg cttcgacgcc agcgtgcggc aacccggtgg ccgtgcacac gcagagcgct
    21121 ggcgggctac agggcatagc gctcgcgtgc accgtgtgcc tgcgcatgac ggagcgcgcg
    21181 gcatgatcga gcagaacctc gcgacggtgg cggcgcactg gtatcggcgg atcgcgatgg
    21241 ccgatccggt tgcctcgttc gtgttcgagg tcgtgccgat cgcgaaggag cgcccgcgga
    21301 ccttccacgg caagcgtggg ccgcgcacgg tgacgcccga gcgcacgaag gcggcagagg
    21361 agcacctcgc gtggcggttt cgtctcgcga tgcgtgggca cacgctcgag ggcaacctcg
    21421 gcatggtgtg cgtgttctat cgccccgacc ggcgccgcat cgacggcgac aacatgctca
    21481 agctcgtgtg cgatgccggc aacatcgcgc gagcgtgggg cgacgactgc cagatcacta
    21541 ccaagctcgc acgcatcgag ctggatgcgg cgcggccacg taccgagatc gcgatcgggc
    21601 cgatggcgtc aactctcacg cgataggagc aaagcgatgg ccgatgtttc agtctgcagc
    21661 gtgtgcaagg agcccgacga ctgccgagcg tgcgcgtgcg agcgttcacg catcaaggat
    21721 cagccgccac cgataccgcg cgcattggcg acagccgtgt gggatctcgt gctcgaggac
    21781 tgcgcgcaca agccaggccc gacatgggag cgcgtccgcc gtgacatgca cgagcgtgat
    21841 caggtcggac gcgaacgcta cggcacccca ctgacgacga acaacgggcg cgatcacctc
    21901 gtcgacgcgt accaggaagc tctcgatctc gcggtctacc tgcgcgcgca gctcgaggaa
    21961 tgggccgaca gcgaccagcg gcggcacgtg ctgacgatct atacgctgca cctcgtgaac
    22021 gtgcaccacc tgcgctcggt gatcgacgac aaggaacagg cccatgggcg atgatctggc
    22081 gcgtaagcta tacgaggcgt ggaacgtcgg cgggcagcac gatcttgcgt ggggcttact
    22141 cgagaccgag aaacgcgagc agtgggatgc ggtggctgtg gtcgcccgca agcacatgcg
    22201 cgagcagatc gcgctgcagc tcgaggccga ggtgcgcgat ctcgacgagg acggcggcgc
    22261 gtacctgcgc gatggcgtgc gccacgccgc ggtgatcgtc cgaccgttct agcttgcccg
    22321 ctgtcacagc actgcggtaa acccgtggga tggggcaaac aacaggtatc agctggacag
    22381 acgccacgtt caatccgtgg tggggttgct ggaagatcgc ggacgagtgc aaaaactgct
    22441 acgccgacgc gacagcgcac cgctacacga gccagttcgg cgagctatgg ggccgcactg
    22501 ccgggcgccg tttcttcggt gattcgcact gggccgagct ggcaaagtgg aaccgcaagg
    22561 ccgagcgcga cggcgtgcgc atgcgcgtgt tcgtcggcag catgtgcgac tgggccgaga
    22621 tgcacccgga cgacgccatc gcgctgcaga tggcgaacgc tcgagagcgg ctctggcagg
    22681 agatccgcga gtgcagctcg ctggacttcc tcatgctcac caagcgcccc gaggatgcgg
    22741 cgcagctcgt gccgtgggct cgtggtgaga gctacgcgcg cgacccgtgg ccgaacgtgt
    22801 ggatcggcgt caccgcgggc acccgcgaca cgctgcgcgt gaacgcgtcg atcctgcgct
    22861 cgatcccggc ggtggtgcgg ttcatctcgt gcgagccaat cctcgagggc atcgccgcag
    22921 acgagtggga cgacgcgatc ggccccgggc atggaaagat cgactggctg atcgtgggcg
    22981 acgagagcgg gcctcgagcc cggccggctc aagtcgattg ggttcgcacg gcgcgcgagg
    23041 ctgcactgcg gcacggcgtg gcgtttcact tcaagcaatg ggcagggcgc gacgtggccg
    23101 gcatcggtgg acgactgccg agcaccggcg cgggtaacgc gcgcaaaatc catctcccca
    23161 cgctcgacgg caagcagtgg gctcaattcc cgaaggagca ctaagaacat gagcaacgca
    23221 gaacgatacg cgcgcgagct actcaagagc gtgcagaaca tgatcgcgag cgcgctgcag
    23281 ccattcgacg agcaagagcg gcgcaccgcg gggctgctcg cgcagacgcg cgacgagatc
    23341 gggcgtgacc gacaggcgaa ccacgagacg gagcgcgacc tacgcgcgac catgcgcgca
    23401 attctcgatc gagtcgaatc gatcgagaaa gacatcaaag cgcgcgacaa ttaccacaag
    23461 cacgagcgcg tgcggttcga gaacctcgtg cacaagctgg atcgcgatct ggcgaccacg
    23521 gcagacgcgc aagcctcgag tgtcgacgcg ctcgccaagc gggtagctct gctcgagagc
    23581 gggcgcactg gacacatggc gctgcgccac gggccaccac cgggcgtgcc gccattcccg
    23641 atcggatccg gcggaagtga gcgcgcgatc gatcgtgtgt ttcctccgta cggcgaggcg
    23701 ttcaaacccg acccgaccac ggacgcgatc gagcgactag cgcgcgagcg cgacggattg
    23761 cgcgtggccg cagaggagat gcgcagcgca gccatgaagg cagagcgcga atgcgaggaa
    23821 aaacaacacg tgatcgtgag gcggaacgag cggatcaacg gcctaaccgc cgaggtgacc
    23881 gcagcagaga agcgcgcgaa ggacgccgag cgcgtgctcg ccgatcgagt caacgacggg
    23941 ctgcgcatcg ccgacgagtt acgcgcgaag ctgaccgaga gcagcgcacg gattcgcgat
    24001 ctcgagcagc aggcgcagcg gcgcacgggc atcgacgagc gcaggctcgc gaagctcgcc
    24061 gagctggagg gcaagtaggg catcacgcgc gcgcgagctg tcagtatcgc ggtggaagcg
    24121 gccgaggccg acacgatcca gacgtacacg agtgctcgcc cgttcgtgcc gcacgagtgg
    24181 gttgtgcggg ccgtgatgaa agctgccggc ggcgaggttg ccgaccccga gcaacccgag
    24241 cccgacgact gccaggccgg cacgtgtgat tgccccggtg atgacacgca ttacccgacg
    24301 agagatcccg gcagcagcgg cgcggtatag acggttgtca caccgctgcg gtaaacgtgg
    24361 cgggtgcgat acgaagatca gcaactgatc catgcctacg ccaagctcgg cctcgagatc
    24421 gcggcggcaa agaacccgca agcccagcac gatgtcagcg aggccgagac gttgcgccgc
    24481 gggtatcagg cgctcaagcg ccgcatggag cagctctcgc cgaagaaaaa ggcgatgttc
    24541 cgcaccatga tgcgcaacga gatcaacgcc gtgcgcgcgc tcatggcgaa ggccgaggcc
    24601 accggcgaga cgttcacgct cgccgatatc tccgtcgaga tgatcgacgc cgagaccggc
    24661 caggccgtgc ccggtacgcc aaacgtgcag ctcgagaccg agagcgcgga atgaacctcg
    24721 gcaccgcact tgtggagccg ccgatcgacg agatcgagcg ctccgatgcg tgggcgctcg
    24781 tgcgcactga ttcgctccgg tggctcgcga cgcgcgaccc tgacagcgtc gacgggttcg
    24841 tgatggatcc gccatacagc tctggcgggc agttccgcgg cgatcgcacg gcgagcgtgg
    24901 tggacaagta ccagcagagc gggcacgcgc agggtgacta cccggaattc tacggcgaca
    24961 cacgcgacgg gcgcgggatg ctcgcgtggc tctcacttgt gttcgcggac gcgtggcgag
    25021 tcaccaaacc gagcggcgtg atcgtcacgt tctcggactg gcgcatgctg ccgacgatga
    25081 cagacgccgt gcaggccggc ggatgggtgt ggcgcggcat cgtgccctgg tacaagccaa
    25141 acgcgcggcg gcagctcggc cggttcactg gcgcctgcga gtttgcgatc tggggatcga
    25201 agggtccgat gcccgttgag cgcgacgtcg gagcgctgcc gggattcttt cgtgacgcga
    25261 acgtatcgcc cgagctacgc gagccgcccg cgtacatcca gagcaacagc gtgccgggcg
    25321 acgatcggca gcacgtcacc gagaagcccg agagcgtgat gcgcgagctg gtcaagctcg
    25381 tgacgcccgg cgggctcgtg tgcgatccgt tcaccggcag cggcaccacc ggcgtagctg
    25441 cactgctcga gggccggcgg ttcgtcggct gcgagctttc gccggaatac ttccgcgtag
    25501 cgtgcgatcg gtttcgcgcg ctcgcgaacc tcgaacaccg caaggatgcg gctcgaggac
    25561 aggtcgggct attcgcccaa aaacaggagc aagacacatg accgcaacag caacagcaac
    25621 ccctgcagga aaacccaagc gcgagcgcaa gccaccggcg gcaatgacgc cgacgaaggc
    25681 gatcgccgat atcgtcaagc tgcgcgccaa ggcgctcgag gccgaggaca cgatcctcgc
    25741 gcagctcccc gaggccgatc gcaagcgcgt gatcgcattt ctcgaggccg agtaacatgg
    25801 ccgtcgcaaa aggagcggat atcccggcag ctcggctgct cgatgccgtc gaggccaccg
    25861 cgcacgcgct aaccgcgttc gaggcgtggg ccggcatcaa ggcgagcgcg atcgagcccg
    25921 gcaccttgca cgccgtcgac gagatgcaga tcaggctcgc ggcgtggcag aacgatcggt
    25981 tcgggcacgt gccgacgtcg gatgtacaca tcgcgctcgg tgtgatcgag gagcttggcg
    26041 aggcgttcga cgaggacgca ggccccgagg agtcgatcga cgcgctcggc gacgtcatgg
    26101 tgtacgcggc gcagctctgc acagcgaacc gcctagcagt gcgcccggtg atcgggctcg
    26161 cggtgcacta cgtcaagacg aacgcgtgcc acgcgcacgc tatcgctctg gcggggcagc
    26221 tcgcccacgt gtgcctcaag catgcgcagg gtatccgcgg gctcgggccg ttcgaggcgt
    26281 accgcccgcg gctcgtggac gcgctcgcgc tcatgatcgc aaaggcgatc gaggactgcg
    26341 cgatcgggca cgagctgacc gttgatccgg acggcgtgtt cgtcgtggtc ggcggcgagg
    26401 tgctcgcgcg caagccgggc gacacgatga tccccaaggc cccggccggc atggtcaacg
    26461 tgcaggtgat gaaggaggcg atcgacgcgg caacggagcc gtttcggcgt ctcgccgagg
    26521 gcgtggacaa gctcgccgag gccgagaaag tggagccggg cgacttccag gcgcctacgt
    26581 tcctgcgcct cacctcgagt gagaagacag aagcgtgccc ggtgttcgac tcggcatccg
    26641 gcgtgtggtg cgagagcgac gcgcagctgc gcgagcgcat caataagcac gctgcagatc
    26701 tctcggcgta gctcgagtgt cacacccggg cggtaaaccc ggggcatgac gtgcgagcat
    26761 tgcaacacgg agcggccgat catgatcgag ctgcgaggcc ccgacggggc accgtggtgg
    26821 ctctgttcgc cctgttaccg cgaaggattc aaagatgcag ctcgtaaccc cgacagcatg
    26881 gatagcggca cgcacgggcg cagtggtgcc ggtgcggcca ccgatgatct acgtagcgca
    26941 cccggtcgcc gcgcaagccg gtgaggcgat cgcgacgtgc accgagtgcg atgcgtcgca
    27001 cctgttcgcg cccggcgacc cgctcgatct gcggctcgtg tgtcagcacg atgccccgat
    27061 ccgtaactcg agcgacccgg cggcgatcgt cgcgttcaac tgcgcccgcg cgctgcggtg
    27121 gtggcggtgg ctgcaccttg ggctgcccga tgtcacgtgg gtaatgccgt ggtacgtgaa
    27181 cgtgatcgcg aacggtgagg ccgatccggt gctgatcgag cgcggtctcc gtgacgattg
    27241 cgaggtagct cgccggtgcg acgcgctcat ggcgtgtggc ccgcggatct cgagcggcat
    27301 gcggcgcgag gcgatcgcgg cggccgaggt cgggaacgag ctgttccaga tcgtcggcgt
    27361 gcaccgcgag ccaccggtct cgtggggcgc gacgtcgacg ccgtggcagc ggtgggcgac
    27421 atgaacctcg agcagctgct cgtatgggcg cgcgccgctg ccgttgagag tcagtcgccg
    27481 tatcgcgaca aagaggccgc gcgcgcgatc ctgacgctgc tcggcgtggc gtggccgtgc
    27541 gggctcgagg agccgccgat cgtctcgatg ggtgacggca cgtgcggcgt gctggtcgac
    27601 aacggcggcg atccgttgac gcccgacgag ctgcgcggat atgccgcgcg gctcctgcaa
    27661 ctggcagagg aagcagagga gcagagcaaa tgacaacgca ggaaacatca cgagagagct
    27721 accgaatcct gatcgagagc ggcaagctgc gcggcaagca agcgcaggcg ctcgaggccg
    27781 tcatgcgtca tgggccggcg acgagcggag agatcattca gacgctcggc accaacgtga
    27841 acctctggcg cgcacggttc accgagttgc agggcaaggg tctgatcgtc gaggtcgggc
    27901 agcgcaagtg caccgtcacc gggcgcagcg cgatcgtgtg gcaatacagc gggcgcacca
    27961 agcctctcga gctgcagcac cgagcgggca caaagcagct acgctcgctc cttcagcgag
    28021 tggtgaaggc ttgcgacgtc aacgggatgc agtttgcgcc gagcaagacg ctgatcaccg
    28081 acatcagagc ggcgctccaa tgagactcgg acccgagccg ccgacgtggg gcgatctggc
    28141 cgtcgaggca ctgatctgga tcgcggcgat cgcgctcgtc gtcgggttcc tcgtgcgcgt
    28201 gctccccgtc gtgctgtagg ctgatcgtgg agccatggca gacaacgcac caaagaaagc
    28261 acccgtcggc ggcggcattc gcccgcccga ctacatccga gagttagcgc tcgcgcgcgt
    28321 ggaggatctt atgctgcagg cgtatagccc gcgcgagatc ttcgccaagc tgcacgacga
    28381 gggattcacc gagagcgagg ccaccgcgaa ggagtggcgc ctcgcgattc aaaagcggtg
    28441 ggctctcgag gacgcagagc agcggccggc acgtaaagac gcatggcggc gccgcatcga
    28501 gagccaatac cagaagctcc tcgagcatgc cgagaccacc aacagcgagc tggcgcgcgc
    28561 gagctacttc gccgaggcca ccaagctcgc gaagctcgcg ctgatcatgg acgggctcac
    28621 ggcacccgtg aaggttgaac actcgggcgc catgatggat ccgctcgcac tgcagccgca
    28681 cgagcgcgag acgcggatcg ccgagctgct cgccaagcgc gaggcggcgc ttgccgctgc
    28741 cgctcgtgcc ggcggcaagg tggtgcattg atggatccgc gctgccgatg ctgcggcatg
    28801 ccgatcggtg cggcgtacgg cgcgatccgg atgcagctgc gcgtgtgcat cgattgcgac
    28861 acgtgcgatc agctgcacgg cgcgcagctg cagggcgtgc tgttcaaggc tgtggccgac
    28921 ggcaatctcg cgccatacac ggcgatcgct cgcgccatga tcgacgatgc gcagctcacc
    28981 ggccggatcg tgcacgacgt gaaggaaggc cgagtcgtcg acatcacgat cccgaacccg
    29041 cccccacggt ggatggtagc cgtcgggcgt gcgatggctc gaggcgagca acccgacgtc
    29101 cagacgctcg tcgacgcggt gctcgcgcgc tacatcggcg gacagaacac gcccgcgctc
    29161 atggccgaaa tgcggatcga gctgcgcgac gcgctgcagt tcgtcgatcc gtcgatcatg
    29221 gacgtcgagg tctcggcgac gatgacagcg ctcgatcctg gcaacctgcg gatcgagatc
    29281 acggccaaga caccgacact cggcagcacg atggcaggcg cagacgacgc ggatatcccg
    29341 cccgacatca tgagccgccc gcgcgccgag gcgtagcacc gtggtggccg ctgccgtacc
    29401 ggcgtgggac gaggagaacg aggacgagtt agcagctctg ctcgccgcgc agcacgaggg
    29461 tgagccgctg gacgcgttca tccgtcgcgt gtcgccgcgg catgcgccac cgcggcatat
    29521 ctcgccgctg ctcgatctct gggaggcgac gcgttacgag cgcgtgttcg catgcgtgga
    29581 gctgccgcca cggcacgcga aaaccaccac gggcctgcac ggcatcgcgt ggcgcctcaa
    29641 gcgcgatccg gccgtcacca acgcgtttgc cacgttcggc gacgactacg cggcgagccg
    29701 ctcgcgtatc gcgcgcgtgc tgactcgagc cggcggcgtg tcgctatcaa aggacatggc
    29761 gaacctgcac gagtggcgca ctaactacgg cggcggcacg atctgcagag gctaccaagg
    29821 cgagtggacc ggccagggca tcacgggcgt gggtctgatc gacgacccgt tcaaggatcg
    29881 cgcatcggcc gagagcgaga agatccgcga gaacgtctgg gagtggttca ccgatgtttg
    29941 gtggacgcgc ctagagccgg gctcgtcgtg catcgtgcag cacacgcggt ggcatgatga
    30001 cgacctgatc ggccggctac tcgcgggcaa gttctcgggg tatcggttcc agcgcatccg
    30061 gctgccagcg atcgccgaga gtgtcgacgg gctcggcccc gatatcctcg ggcggcaaga
    30121 tggtgaggca ctctggcccg aacgctaccc ggtcgaggag ctgcgcaaga tcgaggagtc
    30181 gatcgggccg tacggctggg cctcgctgta tcagcagaga ccgcgaccga agggcgccga
    30241 tctgttcggc gagccggcgc ggttctcgct gctttcgtgg gtgcccgacg gccaccggat
    30301 cctgatctgt gctgactgcg cagcgacgcc gcacacacgc gctgattatt cggcgctgtt
    30361 cgtgctcgcg gccaaggggt tcggctcgga catgacgatc gacgtgctct atggctggcg
    30421 tggccacgtc agcgtgcccg cgttcgcgcg caagctgctc gaggtgtcgc gcatgttctg
    30481 gggcgctccg gtcgtggtcg aggccgtggg cgggttcaag gcggtgccgc agattctcaa
    30541 ggagatcgag ccacagctgc gcgtccgcgt ggctcgcatg ggcgatccga acgaaggcga
    30601 gaaaggagcc gcgcgcgtca gcaacgtgcc ggtggacaag ttcatccgcg cgcagccggc
    30661 ggcagcggcg tggaatcgga gccgtatccg cgtgccgatg gacgccgagg ccgtttggaa
    30721 cccgcagcgc ggacggttcg agatgcccgc tgtgatcatg cggctgcgcg ctggcgtggc
    30781 gcccggcgtg acatgggccg acgagctggt ccacgaggcg ggtaaattct caggcgtggg
    30841 cgacgccgag gatgatcagg tggatgctct ggcgcacgga ttcaacgaac tacactcgag
    30901 ccgcaacgct cgccgaggag cggacgagaa ccgcaacccg ttcgggtaga tgtgaaatag
    30961 ctctcgagag tagttgcata gagtttatag caccgctata aacatactca aaggagcaaa
    31021 gcatgagtgc aaccgtcgac aagatccgca agctgttgaa cctcgcgcag aacgcgagcg
    31081 cgaacgagaa cgaggcagac accgcgcgcc ggctcgccga gcgtctgatg gcagcagccg
    31141 gcatcagcga ggccgacatc gcagacagcg cgacgccgga cgctgacccg ctcgcgacct
    31201 tcggagcgca gcacgtgcgc tcgcccgagg cgggctggca cggcatcctc gcggtggccg
    31261 tcgctcgcgt cgtcggctgt tacgtgcact actcgtggca gtacgagaag ggcaggcgcg
    31321 agcgcgcgct cacgtgggtt ggtacgcagg cgcagcgcga gtcagcgatc gagctgcacg
    31381 cgtggctgat ctcgcaggtc gagcggctcg cgaagggtgc cgcgaggatc gcgaaggatc
    31441 acccggccgg atcgcgtgcc tacctcgggg cgtaccggcg cgggctggcg tcgtcgatcg
    31501 cgtcgcaagc gatggcgctc ggcaagaacc gcgaggccac cacgcagccc gacacgaacg
    31561 cgctggcgcg gcgcagcgcc atccagaatg cgatcgccgc atacgagaag cagcacgatc
    31621 tccgctcgac tggcagcagc taccgcacac gcgcgctcgg ggcgttcgcg gcgggcgtga
    31681 acgatgggcg ctcggtgcgc ctgcagcacg atatggcagg tggcagcgtc aagcggcttg
    31741 gggcgggcaa gtgaggcgcc cggcgctcac gaaggcgcag cggcagcacg cgcgcgagca
    31801 gcgggctctg ctccgtctgc ggcgtgcggc ggtgcggcgc tcgctcgttg gcgacggaga
    31861 gatcgagatg gcggacggcg acgcggttac ccgtgcggat ctcgatgcgg cgtgcgacgc
    31921 ctacacgaac gtgctcacga cgcgcgagcg caggaagctc gcgaagtagt cagcgggcgc
    31981 tgtgcccgcg ctcgtgctcg aggcgagccg cggcggtgtt cttcggagtg cagttgccgc
    32041 accacgcgag acggcagcgc gcgacgcgac agcgcatgaa cgtggaggca ggctcaccgc
    32101 aggtgcactt gatgttccgg ccgggtgcca taacgaggtg atcgatcacc cgcgcacggt
    32161 aaaccggcag cgtgctacgt gtcgagcatg gcgaagaaat cgaaccgcga gaccgtgacc
    32221 gtggggcagc ctatcaggac gcgcgtggtc acatcgccaa gcggcgtgaa cggcggatcg
    32281 gcgctggccg agctgttcga gcgcgaggcc gctgcggcga tcgcgaaggc tgacgcggac
    32341 gacgccaagc tcgcggcggc ggctgccagt gcgccgttct acaccggcag tgcagatccc
    32401 ggcaccgtcg tgggactcga cgggctcgca ccgagcagcg agactccgaa ggatggcgac
    32461 tccaagcggc ccgatactga cccaagctga tcgcgagctg gcgggggcgc cgcgcggccc
    32521 cgagcttgct gcaggcgtag cgcagcgcat gcgagaccgc gcgccgatcg gcggcggcac
    32581 ggtgacggtg gctctcgccg ctccatcggc aatgacgtgg agcgacttcc agcagcggca
    32641 cccggaatgg cgcggcgact actgggccga atgccgcgcg ctctacgctg gcggcgatcg
    32701 gctgctcggc gataaagccg tcctcgagcg gctgttcccg atgcacttgc acgaggtgca
    32761 gaagatctac gaggagcgca agaagcgctc gcattactac ccctatgcgg gaacgatcat
    32821 cgatcaccta ctcgccgggc tcggcaccga tccgctgcgc gtatcgttcg cgctcgagga
    32881 cgatcagggc aagcgcgagc tgccgcccgc ggcgaagtgg tgggagcgtt gggtgcagga
    32941 cgtcactgat acggccgagc gcccgagcga ctacgggctc gaggatgacg acgacggcga
    33001 ggacgacgac gaaggcggca cgacgatgca ctcgtttatc gtcgacgtgc tgcgcgaggc
    33061 gctgcagacg cgcacggcgt gggtgctcgc cgatctgcca ccgctcgatc ccgatgcgcc
    33121 agcgccgatc atcgacagca agctcgcggc cgagcagctc ggcaaggcgg atccgtacct
    33181 gtgcgtagtg ccggccgagc aggtgatcga ctggcagacg agcgacgacg gaaagcgcct
    33241 cgagtgggtg atcgtcatga cgcaggagca gatccggccg tcacccaagg agcggcgcgg
    33301 tctcgtgcgt cacacctacg tgatctggga cgccgaggtc tcgacgaagt atcagatcga
    33361 catcgatccg gcgaatctgc cggcggcaga gcacccggtg cccgtcgcgt caggtccaac
    33421 gccgcacggg tttggccgcg tgccgatcga gcggctcgtg ctgcccgagg gcctgttcgc
    33481 gatgggcaag ctccacagcc tcgcgcgcga gcacttcaac aagcgttgtg caatgtcatg
    33541 ggccgaatac aaatcgctgt tcgcggtgct ctacgagttc ctcggccccg aggacggtac
    33601 gggtatgccg gtgccaatgg cgcagatgga tggaaaccgc gcggcgaatc agatccgtgg
    33661 ccagggctac acgcaggtgc gcggcaagga tgacgacgcg cggttcgtcg ggcctgacgt
    33721 ggcgccgttc aaggaggcgc gcgagagctg caacgacgcc atgcgcgaga tgcaccgcgt
    33781 gatgttctcc atggcgctct cggcgaacat ggacagcgca gcgctacagc gctcgggcga
    33841 cagcaaggca caggacagcg ccaccaccgc agtgctactc gatgcgtttg gccagatcgt
    33901 gcggcggttc tttcgtcgca tgctcgtgct cgcggcgctc gggcgaggcg aggcgccgcc
    33961 gaagtgccgc gtcgagggcc tcgagcaatt cgacgtctcg ggcgtggcgg acgcgatcag
    34021 cgaggccgtg gagctgctca acggcgtgcc aatcctctcg ccgctgttcc gcgagctgtt
    34081 cctcgcgcgg ctctacacca aggtgctcgg cgacgtctcg cacgagcaga cggcgaagat
    34141 ccgcgagcag atccgcgacg cgatcagcgc cgaggagacc gcgatcgccg gtgggctgat
    34201 cccgggcgtg gcgcccggcg ggcctgcgcc cggtccaaag aacaagccgc agctgcccga
    34261 ggacgcagag cccgacgaag acgaagacgc gaaggacgcc aaggaaaagc agccacgggg
    34321 ctacagctca aagcgcaagc ccaagcggta gcgcgtgccg actgccgcgc aggttcaatc
    34381 cgtcgagcgg ctcgtgcacg aaagcgtgca acgggttgac gcgatcgcgc ccgaggcgct
    34441 gcgctcgcta ctgccggcgc tgcggcaagc tcgagacgag ctgcgcgcgg atctgtctcg
    34501 ctggctcgcg acagcccccg agggtagcga gcggttcacg gcgtaccacc gcgcgcaggc
    34561 gctccggtca ctcgaggcga cgttcgagcg gatcgaggat ctgcatcctg cgatggccgg
    34621 cgcgctcggc atgggccggg ctgagaccgg cgcgctcgcg gtcggcaacc tcgagcacga
    34681 ggtgcagcgg ctctcgtcga tcttcggtgg cggcatggtc acgctgccgc agatcgacac
    34741 ggcggcagtg ctcgcgcagg gtaacaggct gctctggaag cggcacgaga acagcgcccg
    34801 gcgctacgcc ggtagtctcg gcgatgacgt cagacaccgg ctcgcggtct cgctcgcgaa
    34861 gggcgacacg ttcgatcaga tggctcggcg actgcgcgga tcgtccgagt tcgcggcgat
    34921 ggtcgcgcga catgacccgg gcgccgcggc gttcggcgtg gcggacgcga cgttcgcgcg
    34981 gtggcggcac tgggccgatc gactcgtgcg caccgagacc atgcacgcat acaacgtgca
    35041 gcacgacgca tcgatcgaat acgtcaacga gcagcggcac gaggatgacg aggagtatct
    35101 gcggcgctgg gatgcgacgg ccgacaaggt ctcgtgcgag cggtgcaagg ctctcgatcg
    35161 caccgtcacg acgatcaagg gcgattttgc gggcggcgtt cactcgccac cgctgcaccc
    35221 gtactgccgt tgcgtggtac tcgcgtggct cgctcggtgg ggcaacatca agggcgaggt
    35281 gccggcgctg cacaagggcg gcgacgaaga aatcatcccg caagagcaca tcgagaacga
    35341 gcgacgcgcg aaggcgagca aggaagcgag agccgcagcg gccgaggatc tcgcggcgtt
    35401 cgacaaacac gaggccgaca agcgcgaggc cgcggcgaac ctgcaggcgg cgaaggagcg
    35461 gcaagcaaag cagctcgcgc agcacaagga gaagcaggcg gacaaatccc gcgagcaggt
    35521 ggcacccgcc gaggcggtgc ccgtcgagtt caagcagaaa aagaatccca agcgcgtggc
    35581 agccgcaaag aaagcggccg aggcgtcagc ggagcggcgg cgcgagatcc actcgacggt
    35641 gcgtgatgcc ttgtccccgg agctgcaagt ggcgtgggac aaggaggggc acaaattcat
    35701 gcgcgagcag ggaaagcgca tcaagggcgt gaaggatcgg atcaacgcat cggcaaagct
    35761 ctccgaggcg ttcgccgaga cctatggatc cggcgagcag acgatccacg gcaacgaggg
    35821 agatcgctcg cacaagcgcg ccgagcttgt cgcgcagcac gcagagcact gggccgacga
    35881 gcaagagcat aagcactacg aggccgagcg cgcgcgagcc atgcgcgacg gtctgatcga
    35941 cgagcacggc aacccgacgg cgaagggcaa ggcgcagcac aagcacgagg atctcgacga
    36001 ttggggcacg ccgatcacga aggtggacga cgatgatccg ccattctgat acagtaaact
    36061 cgatgctcac cgggcaggcg cacgccattg tcttgcgcag ggttgcgctc actacgttga
    36121 gcgatgcacg agcggcgtgg aagcgcgccc gcgagcggcc gaacgagaag cgaacagagg
    36181 cggcatatcg tgcgtgcgtg ctggcagctc ggatgctgcg caaggctgca gcggcgatcc
    36241 cgcgagaggc caagcagatg ctcgaggcgg cgacccggat cgaatcgacc ggcgacgagc
    36301 tgaaggcgca gctcgtgagc atgctcgagg ccagtgcaga tcccggcacc gttcgctagc
    36361 ggtgatctgg cctcgctcgt cgtgcaagat ggcgggcaga ggagcaaaga gaaaccatgg
    36421 gtgcgaagaa gaagccagga cagcagggta atcaggcgcc gcaagacgac gaggacgacg
    36481 acgcaggatc gggcggcggt ctcaccgacg aggcgcgcac cgagatcaac acgctcgtgc
    36541 acgccgcgat caacgcgcaa ttttcgcgaa agctgccggc ggcgatcagc tcggcgatgg
    36601 agaccgtgat cgccccactg cgcgagcagc tcgccaactc gaaccgcgga cgcgcggccg
    36661 agggcgacga cgacgagggc gacgacgatc aggatcagca gcccgcggcg cgcaagaagg
    36721 gcaagcagcc cgcggcgcgc aaggatcccg aggtcgagaa catgcgcaag cagctcgcca
    36781 agctcaccga ggagcgcgag caggagcgaa cgcaagcacg caaccgcgag cgcgacacga
    36841 tgctgcgcga gcagctcgag gcggctggcg tcgataagaa ccgcgtccgc ggcgcgatcg
    36901 cagtgctgcg cgattcgatg aagtacgacg acaaggccgg cgagtggatc tacagctcca
    36961 agcgcgacgg atacgaggag cctctcgacg tgtccacggg cgtgtccgag tgggccgcga
    37021 ccgacgaggg caagagctac cttgcgcccc cgagcaacgc tggacagcca cgcggcggca
    37081 gcggcatgcg acccggcgca ccgaacggcg gcgcacctcg agtgcagaac ggcggacgcc
    37141 cggcgcagga tccgaaggcg gcgaaggcgc aggcgaaagt cgacgcggtt aagagcctct
    37201 cgacggcgat cgattcgctg gccggcggcg tgatcccgct cgggtagatc cccggatctt
    37261 gacgcgcgct cccgcgcgtg tagtgtgtag gtctctactc gacgagggct tggagcagcc
    37321 taagacgagg aagctcgacg ggggaatacc tcggacgcac gcggcgcagc cggtcagcct
    37381 gcgcgtgaaa cgtccgatca accaccccgg agtgctccta tgtctcttgt cgcaatgacc
    37441 ctgatcgcca actcgctcac cacgattttc gaggagcaga tcacctcgca gatcaaccga
    37501 gcgacagtgc tcttgcaggt gctccccgtc gggccgggca ccactggcaa gaacatcagc
    37561 tggaacgccc gcttcggctc ggccgttggc ggtgcacgcg ccgagggcgc tgcagtcgtc
    37621 acgctcaaca acgacacgaa ggtgccggcc accctcgagt tcggcaccta cgatgacgcg
    37681 ttcggcgtca ccggcaaggc gatggcagcg gctcgcgcgg ccaagaaccc ggaggagctg
    37741 gccaacctgt tcgtcgacga gctgggcgat tgcgtggagc gtctggcgaa gggcatcgcg
    37801 gtggacctgt acacgggcac cggcgcgacg gaccatatct tcggtctcgt cgccacgggc
    37861 ggcggcatca tgaacaccgg cacatatgcc ggcatcgatc gcgccgtccg cacgcagtgg
    37921 cagggcacgg tcatggagaa cggcggcgta aaccgcgcgc tcacgttcga tctgatgcgc
    37981 gagatgcggc gccgcatcta ctcggcatcg ggcctcaagc ctgatctgat cgtgaccacg
    38041 cccgagctgc acgagaagta cggcaagctc tttggccagc agcgccgcta cgtgcaggag
    38101 atccgcctcc gcggccagac gatcacactc gacggcggtt accagatgct cgagttcgac
    38161 ggcatcccgg tgctcgagga cgtcgattgc ccggccgaga acatgctgtt tctcaacacg
    38221 cgatacgtgc gcgtgctgca gctgccggat caggtcgaca gcgtcaacaa cagcatgagc
    38281 atgatcggtc tcgcgggcac gcctgaggag cagctcggcc agccatcgag caagctctcg
    38341 gcgcgcatca acccgcttgg gcgcgatggt gattcgtaca agttccagct gatttgctac
    38401 ccgcagctgc aggtgcgacg cccgaacagc tgcggcgcga tcatcgatct cgacgcgacg
    38461 ctctaagcgt caacgcgcgc gccgtatagc gcgccgtgat cgctcggggc gcaccggccg
    38521 agcctcgcgc tctatgccgg tgcacctcgg agcaaatcac gatgcgttct ctctctcgat
    38581 acagtgacga ttttgatcgc gatgcagaaa tgcagagcga ggctcgcgtg atcaacgtgt
    38641 cgccacacgt ggcacgtttt cagatcgcga ccacgccggg ctcgcagccg cggcgctaca
    38701 agctcggccc cggtgagtcg atccacatcc agatcggcta cacgcaatcc ttccagggtg
    38761 cgggccgtca gccggtgcgc gcgacgatcg agtcgctgac ggagcgcgag gtctggaaca
    38821 aaggccccac gctgccgatg gtcgtgcacg agtctcgagc cgcggagatg cgctctcgct
    38881 ggcaggagat gatcgcgcgt ggcgcggaac cgcccaagcc catggacgtg tacctgccga
    38941 gcgctgacgg tggcgagccg gtcaagatga cggtgcagcc ggcggcagcg gtcacgcccg
    39001 cgcaggttgc cgccgcatcg tcgcggctgc ccgcgttcga tgacgacgag gatcagagcg
    39061 cagcggctct cgacgagccc ccgcccgatc acaacgagcc gctcgaggcc gtcacggttc
    39121 cggctgcgcc ggtgccgacg aaggccgaga gcaagggcaa ggggcgctaa gccatggcgg
    39181 tgctcaccga cgaggagaag gcatccatcc gcatgtacat gggatggagc gcccgattcg
    39241 cgcagaccga cacagctctc gagcgcgcca tgagcgcgat cgatgggatg ccggcggatc
    39301 tcgcgctcgt tcgtgtgcac ctcaccgagc tggcgcggat cgacgcggcg atccaggcat
    39361 gcgaggcgcg gttcaaggcc gatcaggtcg gttcgatcac gctctcgcag agcgagctgc
    39421 cgcggctacg gtctcgaggc atggagcgcg taggccggct ggccacgctg ctcggcgtcg
    39481 aggtgcgtga caacgctttt agccctgact tgccgcggtg gcgcggcgga catggtgggc
    39541 cgtcgggagg cggaaactac cagatgcagg gctgatgtct tttcggctgc ggtggtgggg
    39601 cgcgccttgc tccacgcaaa ccgccaccgt ggccggatct ttttctcgac gacaacaacg
    39661 ccaaccggag cccgatagga aaccaccatg ctgccgatga tcatcacgaa ggacgaagcc
    39721 acccccgcgc agcgcacggt ggttttctac gccgtcgacg cgaccgacgg gttcacgccc
    39781 gagacggcga tcgatttcag ccaggccggc gacgtcaagc tcgccaaggc gggcggcgcg
    39841 ctcgcgaatg ctgcaggcgt tgtgaccgag ctggcatcgg gcctctacaa gctcgtgctc
    39901 gctgcggccg acgtcgacac gctcggcgat ctcaccgtgc tgatcacgaa tgccggtgtg
    39961 cgtccgatcg ttggtcacct gcaggtggtc gcgttcgatc tcaacacggc gacggtggat
    40021 ccgagcgctg ccggcatcac gtcgatccag gcgggccttg ctacctccgt cgaggttgct
    40081 gcgatcgagg gcggcacgtc gtcggcgttc gtcgccatgg gtacccgcac cacggacggc
    40141 aatttctcgg cgttctcgat gctcggcgcc aagcagacgg tgatcacggt caccggcacc
    40201 tgggacggcg caacggttac cggccaggtg tgcgaggacc cgagcgcagc ggttcccgtg
    40261 tggaccgcgg ccggcacgcc gatcacggcg aacggcacga tcacgctcac cgggccgcac
    40321 aaggctttcc gtgctgccgt ctcgaacgat ggcgcgggca cctctctgac ggcgacggca
    40381 gccgtagtga cggcgctgta aggcaagccc gatggccgtc cctgtcctca ccggcgcagc
    40441 cacgctcaac acgagcgtgc tcgtcgactc gcttgtgccc gacgtaatcg acgggctgcg
    40501 cgaggatctg cacccggctt tcggcgtgcg cgcgtatcgc gtttatcgcg tgatccgctc
    40561 gtgggccggc aagacggtgg gggaggggcg ccacaccgac gacgcggccg agctgcgccc
    40621 gcaaccgcgc gtcagcgtgt ggagcgggct caagtacgtg caggcgacgt gcggcatccg
    40681 cgagcttggg gacgtgcagc tctccgaggt ctcgctcacg tacaccgcag agcagctcga
    40741 tccgcgcacg ttgcgcagca atcaagagct gttctatgcg ctcggcgagg cgaacggcca
    40801 ggccacgccg ctgcgcgtgt gggcgcattc cgcgcccccg ttcatcgatc gcgaaaagac
    40861 gatgggctgg ctcctgttcc tgcgccgcgt agaggctgcg ccagcgtgga gcccgtcgtg
    40921 agcaacacga tccgtgtggg gctcgatcag ctgccggcat ctctcgatct cggcgagcga
    40981 acgatccggc gagccatctc tcgaggtgcg cttgctggcg cgcatcgtgg ccgcgccgtc
    41041 atggtgcgcg cgacgccgac ggatcagggg cagctccgcg cctcgtggaa agtgaaggca
    41101 ggcgccgagg aattcgacgg cgtgggcgcc aaccttgccg agctgatcaa cgatgcgccg
    41161 catatctcga tcgtggagct tggcgctcgc ccgcacgcgg tgagccgcga aggatgggag
    41221 gcgatctacg attgggtacg gcgccacttc cgcagcgagg gcggcgtgca tgttctcggc
    41281 ggcggcgggc gcatggtcgc gcgctccaag gatggccggc gcgatccgcg catcgaggcg
    41341 atcacatggg gcatcgtccg caagatcaag aagtacggcc agaagcctac gctgttcgtg
    41401 cgtaacaacg tcgacacgct gcgcgaggtc atggcctacg agctacagcg cgagctgcag
    41461 cgcgcgatcg cgaacctcac gcccaagggc ggcaagccgt gagcggcgtc gtgcgtatcg
    41521 aggcgctgcg cgcgttcgag gcgctgatcg aggctcgcgt gcccgagctt gcgggccacg
    41581 tgtgcaccgg cgtggctccc tcgagcgagc acgagaccgt gccgaacctc tcgatccagc
    41641 cgacgaaatg gacctacgag ccgaaccagg ccggcgagca cacgacgctg cccggcaatg
    41701 tcgtggtctg ggacgtcggg cagcacacgt gcgcagcagt gctctcgatc gtcgcggcgt
    41761 caccggcgca gcgatgggag atcgaggcca aggtgctcga tctgttcctc tcgagcgtgc
    41821 acccgctcac cggcatgcac cggccgggcg tgatcgtgct gccggtcagt gcatgtccgc
    41881 agctcggcgc atggttcgcg gcgttcgagc tggagtcgga cgagtggagc gacacgctcg
    41941 cgctcgatcg ccgctacgag tctcggatcg tgatcaacct cgtgatcccg gcgctcgtga
    42001 ccgaggcgcc gatctacacg atcaacgagc tgatcctcgg cgtcaccgag gacatgacaa
    42061 cagtgtttac acctgccacc gcgatcccgc ctgcggtgga gttggtgtct atcaatgagg
    42121 ccggggcgat cactcgatac gagtaggaga ccaccatggg cgacgtttac ttcaccacca
    42181 atcccgccga gttctcgcgg ctgcccgggc tctacgtgtc cgagcggctg ccggccggct
    42241 tcattcgcgg cgttgatctc ggcacggtcg gcatggccac caagtgcgtg cgcgggccgc
    42301 tcacgccagt gctgatcacg tcgtcgggcc ggttcaccga ggtgttcggc ggacgcgact
    42361 acggcagcgg cggcgccgcg atcggccagg gctggctcgc gctcgtcaac aagccattcg
    42421 gcacgctcgt gatccgtcgc gtggccgcgg ccgatgccgt caaggcatcg ttcaccgacg
    42481 agaccgcggc cggcggtgct ggcaccgcgg tgctgcgcgt ggacgcctcg agcgttggcg
    42541 cgtggggtag caacgtgaag tacaagatcg gtgcagccac cgacggcgac gccaacaagt
    42601 tcaatttgac cgtgtcgtat ctcggcgagc aggtgctcta cgagaatctc gacattcacg
    42661 cggccaacta cgacaacctg gcggcggcag tcggcagcga cgacgcccgg ttcgtcgatc
    42721 tcgtgaaggt cgccagcggt cgcccgaaga acacgatcgc cggtgcggat ggcgcggacg
    42781 cggacggatg gatcgcgctc ggcacggtcg ccgcgggatt cacatcggta gccggctccg
    42841 agggcacgct cgcggtgggc gactacaaca gcggcatgga cgatctcgcg gtctacccgg
    42901 gcgtgggcat ttgccttgtg cccgaggccg tcacgggctc ggcggtcacg ttccacagca
    42961 accttgtgac gctcgcgagc gaggtgtcgg atcgcatctt cctgacgtgg gcgcaggctc
    43021 acggccagag cgtgagcgcc gaggccgcgc aggtggcggc gcagatcacg acgcgcagcg
    43081 atcggatcgt gtggtgctac aacagcgcat acacgctcga tccagagacg gacagcgagg
    43141 tgcagaccgc gccccacgtc tggctcgcgt cgatcctctc gcagatcgac gtcgacgtgc
    43201 acgcgggctc gttcgagacg gtcgcgtatc tcgcgggcat cacccgcgtg acgaacacga
    43261 gcctctctcg ccccgacctg atcacgctgc aggacgccgg catctgccaa ctcgagcgca
    43321 ccaccgacgg gttccagttc cgctcggtcg tgacgacgga tctcaccgag ggcaagacgg
    43381 agcttgctcg gcggcgtgcg tgcgactatc tgcagctctc tgcagctgat cgcctgcgct
    43441 actacgtgaa gcgcaagaac acggcgacga accgagccgg cgcagctgcc gagctggtcg
    43501 cgttctcgga ggatctgcgc gcgcgcgagc gcgtcgtcga ggactacgag atcgatcaag
    43561 agagtgtgaa caacgccacg cagcgcgcgc gcggcgagga gcgcatcctc tggcgcgtgc
    43621 gcctgatcgg ccacatcctc tctctcgttc tcgagaccga catcggcacg ggaacggtta
    43681 tcgagcgcta ggccgcgccg tcacctctca ccactaaccg caagcaaagg aaacgaccat
    43741 gtctcaaaaa atgcgcggac aagagctgac gatgcggatc gccgtcgacg gcgttgtgca
    43801 gccgggctcg atgttcaagc tcacggattt tacggcgacg cctcgcactg agatcgtcga
    43861 ggacgactac ctcggcgaga aggagaccga tctcgatatc caacaccacg gatgggacgg
    43921 cgcgttcaat gtcgattacg aggacgcgca agcgatcctg ctctgcgagg acattctcgg
    43981 gcgcgaatcg aacaacgagc agcacccgcg gatcacgatc accgtgatct atacgtttcg
    44041 cgatccgacg gtgcgcggca agatcgccgt ctatcgcgac gtgttcctca agcaagacga
    44101 ggaatccgcc ggtggccgca aggagaaggt caaaggcaaa ttttcgttca agtgcaagag
    44161 ccgcgtgatc atgaacgcgt gagcgcctga cgccgtgccg gcgcggtttc accggcagcg
    44221 aatggagcaa gcaacatggc aacgaagatg aacagagcgg cgccgccggc cgcagccaag
    44281 cccgatatgg gcgacgagct gcgccccgac gacgtcaccg acagcccgct cgcgcggttc
    44341 tctcgcgagc tggcacagcg gtatccgcag cgcatgatcc ggcgctacga gatgccgacg
    44401 atgatccgtg aatgccgcga ggtattcatg gtcgagatca cgagccgcga cgagatcgag
    44461 gccgcgatca tggctgacgc cacgatgacg agcctcgaga aatcgtcgat ccgtctctcg
    44521 gctgacgccg agcggcgcga gtgcatccgg ctctcgatcg tcgggctcgg caagggctcg
    44581 cccgtcgcgt atcaccacgt gaacagcgac ggcgaaccgt tcgccgagat caacgagtgg
    44641 agcggcaagg cgtggacgct gctacaggcg tacttcggta ccctcaacgg tgtgcccgcg
    44701 gaggagatca tcgaggggct aaagggggcg cagaccgtcg gcgcgttcgc tcccccgaca
    44761 agcgcgaccc gcgcaagcgc cgagagtggg aagtaaggcg gcagctgcac aaagagtggg
    44821 cgcgcctcta ttggcacgga tgcccgtgga cgttcgacga gttcctcgcg cggccggtca
    44881 cggatcgata cctggccaac gcggcgctct cggacctgat cgagatcagc aacgaacagg
    44941 gcaacggacg cccgaaggat gaccgataag ccgtgagcta ctccaccgtc tacgatgttc
    45001 gcgtgcgcta ctcggtcgac aaccgagcgg gccgcacggg cacgcagggg ctcacgcagg
    45061 acgtgcgcac gctcggcaac gaggccaagc gcacgagcgg tgtcttcggg cgtctgggcc
    45121 ttgccgtggc tggcgcgttc ggtgcgcgag ctgcgggcaa ggcgctgatc gggttcaact
    45181 ccaccgtaca agacacgaag aaccagatcg cgggcatgct cgcgctctcc aagaaaaccg
    45241 atctatcggc gcaggtggcc gtcgcggatc ggctctacgg gcgactgcaa acgcgcgcca
    45301 agtcgctgcc cggcaccacg caggaatacg tgcagatgct cggcatgctc acgcagccgc
    45361 tctccaccgc gggcgcgacg ctcaaggaga tggaagatct caccgtcaac gcggtggtgg
    45421 gcgccaaggc gctcggcatc tcgtgggacg ttgcagcgcg cgatatcgat caagcgctgc
    45481 gcggccagta tcacagcgtc gatcagttca ccggaaagat cctaggatcg atgggctacg
    45541 gcggcgagga aggccgcgcg aagttcaacg gcatgagcgc gggcaagcgc aaggaagtgc
    45601 tgcaggctgc gctcatgcaa aagcagctca cgcagctggc agcggcgcag ggcgcatcgt
    45661 tctcgggcat gctctcgacg ctgcaagaca cggtcgagca gtttctcggc aaggtgggcg
    45721 cgcctctgtt ccgtgtgatc acgaacgagc tgaagcagtg gaatggatgg atcgaggcga
    45781 acggcgacaa gatcgacgcg ttcgcgagca agctcggcga gggcctcgtg accgggttcc
    45841 ggttcgtcaa ggacgcgctc tcgttcctcg tggaccacgc ggatctcttg ctcatggtcg
    45901 gcaaggtctg ggccggtgtg aagatcggcg ggatgctcac gagcggcgtg ggcaagctcg
    45961 gcggcgcgtt cggcgcaggc ggcaaggggg ccgggttcct caatttcttc aagggcgcgc
    46021 gcgatggcgt cgacgagaac ggcggttatc agtacacgcc agcaggccgc ggacgctcaa
    46081 aggtcggcgg gctgaagggc gcgatgggca acctcgagct gctcggcggc tcggctgcgc
    46141 tcggctacgc gctcggcaac gcgatggggc tcgacgaggt gggcaagagc gtgggcgcca
    46201 agctggcgtt tctcacgggc cgcgcagacg gcaccaccga ggcaatggac aagctcaagc
    46261 tcgagaccga tcggctcgcc gaggcgacgc gcaaggctac ggcggcaaac ccgggcgcaa
    46321 ccggctcgat cggcatgtcc caaggcgtct cgcttgattt caagcagcag gcggcgctcg
    46381 tcgagggcgt gatcaaggcc gagcagaaat acaagagcga gggcggcatg gataacatgc
    46441 agtggctcgc gcagcatcag atggacatgc aggcggcggg catcgactcg tccgatttca
    46501 agaagtacgg cggcgcgcaa ggtttcgtcg acgcgatgcg cggcaaggcg accgagatcg
    46561 atgcgcgtaa cgatctctcg ggcacgggcg tgctgatcgc gatgaacctc ggcgtgcaga
    46621 agctcaccga gtatcagcgc aagacgctcg agaccgcgca ggcccaagag gagctgagcc
    46681 gctacatgac cgtgcagctc tctcaaggca agctgatcag ccccgagaca gtcatggaga
    46741 tcctgcgcca caacacgcag gatccgacgg gctcgcacaa gtcgatcgcg gacaagccga
    46801 aagtgagcgt gaacatcgcg cgcatcgagg tgcagagcga cgatccggat cggatggcgt
    46861 tcggtctgat cgaggcattc cgcgacgcgg ccaagaatcc gagcagcgca ctctcctcgc
    46921 tgcgagaggg ctagaccatg gcacggcata cattcgtgat cacggagctg cgcagcggta
    46981 ccgatccgag cacggccgac gagggcgagc ggttcgagtg gacggcggat cgcgatccgt
    47041 ccaaaccatt cgacggcagc aagggcggtg gcgctcgcgc gtgcccgatg aagccgtggc
    47101 cgttcggcgg acagcagcgc agctcgcgca cggactaccc gggcgctcgc tcgccgagcg
    47161 agcaggtgct cgggccggtg cgtaagccgt tcacactctc gggcaagtgg gatgatcgct
    47221 acaacggcga cggctacgcg ctcgccgaga tggagcggtt cgaggcaatg gtccgacgcg
    47281 gcaacctctg ccggttccag tacggcccgc tcgcgctcga gggccttgtt gtcgactggg
    47341 atctcgaggt caacagactc gattttatcc gctactcgtt ccaggtctcg acgcaccaca
    47401 agcccgacga gaagaacctc gagcgctcgc cgaagacgcc gagcgagccg atcgcgctgc
    47461 tcgacgagtt cgacaccgca gtgcaagcga tgctcgacgc tgacacgagc gcgccgcgcg
    47521 cgctggtcaa gggcacgctg gccgacgaca tcaccgagca gctcgtcgag atcacgacgg
    47581 cgcgcgagcg cctggccacg acgatcgaga accgcgacag cgaggcgccc gagcagccga
    47641 tcgacgcgtt cacgcggatc gctactcagt tccgcagcgg acgcggcgca gcctacgatc
    47701 tcctcctcac gctcgccgag gtgcgctccg atctcgacat ggccggcggc acggcgatcg
    47761 cggtgctcga gttcgaggac tggatccgct cgctgcggtt cgctgctcgc atcgccatgg
    47821 gcaagggcta cgacggcgat aaggcggcga cggagcgcgc agagcccaac gccgtgcggc
    47881 tgtaccgccc gcgcgcaggg gagaacctgt acgcgatcgc gcgcaagttc tacggcaccg
    47941 ctcacgcgtg gcgcctgatc tacgaccgca acgatctgcg ctcgttcgag ctggccggca
    48001 ccgagacgct gatcatcccg gagcgcgggg cgtagtcatg acggccgggc gagtgcagct
    48061 ctcgagcgat ggcccgcggg tgttctatcc gcagtgtcgc gcgatcctgc aggtgatctt
    48121 cgacggctac ggcgcgagcg ttcgagactc ggagatcctc gtgatcccgg tgctgcccaa
    48181 gagcgtgacc gtgcacatca actcgtataa gcaggccgac agttacgaga tggttttcga
    48241 cgctggcgat ctgccgatcg atccgcgtct cattcgcgcg ggcgcagcgg agatctatct
    48301 gttccaggcg ccgagcgcgg acgatcggac gcgtgtgatc tcgaggcgcc agccgctcgt
    48361 cgacgcagac ccgggcgggt tgcgacggcg cgacccggtg cagacgctcg cgctcgagat
    48421 gggcaccgag ggcagccgcg acaagttcac gctcggcgag cgcaagcctc ggatcgccgg
    48481 cccattcgat gacaccgata tcgagctttc gtcgtcgggc aagtgggtga cgatcaaggg
    48541 gcaggattac accgcgtttc tcaccgcgat ccagtatccg ccgaacccga acggcaccgc
    48601 gcgacgcatc ccgacgggcc ggcggctcga tctgatcgtc gactcgctgc tcgaggaagc
    48661 agatccagat cggcagctat ccgttgacgt gcgcgggttc aacccggccg agatgccgat
    48721 cgtgggtgcc aaggagacgc gcggcaacaa gcgcggcatc ccggtggaac agggcacgag
    48781 ctactgggac gtgatttaca agctcgtcga gaggcatggc tttatctgct acgtcgacgg
    48841 gctcgacgtc gtgatcagtc gcccgaagac gatcactgat cgcgacacgc cgaacatcaa
    48901 gcgcatggcg tggggaaaga acctcgagca cctctcgatg cggcggcatc tcggcaagga
    48961 gcaagcgccc acgatcgtgg tcaagagcta cgacgagcgc acgggcaaga cgctgacggc
    49021 cgagtatcca gacggctcga tcgatcgcgc cgtgatcatc gactccacca agggcggcaa
    49081 gcccaaggtt caccggcggg taaaggagtc gacgcggcga tcgaagaaaa ccggcaaggt
    49141 caagacaacg atccgcgagc gcgacgaata ccagatcgtg caagtgaacg gcatcaccga
    49201 tccggcggcg ctcaagcgga tcgccgagaa ccgctatcac ctactcggca aggccgagcg
    49261 cagcgtcacg gcgacaacgc gcgatcttca cgatctgcgg ttcgcggaca ttctcaacgt
    49321 gtccgcgggc gatgcgttct ggatcgagtg ggacgagttc aatcgcgagc tgctcgcaaa
    49381 cccgaaggtc agcgaggagc aaaaggtgcg acacctcgag ctgcgcgggt tcaactcgga
    49441 gatcgcgcgc gaggtggcca agcgttacga gctgctcgct gggctcgatc gtccgctgcg
    49501 attcaaggag ggcacgatcg agttcgacgt cgaggacggg atcaaaatcg agatggagct
    49561 gcaggacttc atggtggtgg aaggaatccg cagcgacagc ggagccacgc gcacgccatt
    49621 gctgcagcaa aagcgcgagc agcaaaccaa ggccggcggc aagccgatcg ggtggagcga
    49681 gcagtatcgc aaggcgcagc aacggcggtg gcagcgatga gaatcaccaa gccaaagcgc
    49741 ggacgcgggc cggtcaagcg gatcgatctc accgatatgc gcgagctgtt ccgtgatcgc
    49801 cgcatgtggt cggcggttgg aatcgtcacg gcgcccgagg gtgagcctta ctgggaagtg
    49861 gtcaccaacg aggatggcag ctcggtggac gtcgtcgtgg aggtcgtgct gcagccgagc
    49921 gagcagcacg taacgtgtcg gctgcgctct ggcatgtggg aggtgccggc cgagggcgac
    49981 gaggtggcgg tgctcattcc agagggccag atcgatttca tgccgatcgt aatcgctcga
    50041 ctctcaggca acgccgtgcc gacgacgcag gggccgagcc cgacacggat cgcgatcgtt
    50101 gcacctagcg gcggcgaggt ggttgtgcat gacggcagtg gtggcgccga tccgctcgtg
    50161 cgacgaagcg agtttctcga tcacgcgcac ccgacggctg ccgcgggcgc gccgagcgcg
    50221 ccgcagcgca taacgggcga ccccgacgcg gtgccgccga tcgccccact cgcggtgggc
    50281 gccgtcggat tcccgggcac cgacgtgctc aaagcgaagt agagcagatc ccccgatcca
    50341 gggtaccaag gagccagcac atgaaggcaa cagcaattta cgcgctcgct ctgatgctcg
    50401 cgctcaccgg caacgcgctg gccggcggga ccgtcatcac gcagcccgtc gggcagtcgc
    50461 gtgtatcggg cacatgtcca cccggctcgt cgatccgagc agtgaacgca gacggctcgg
    50521 tcgtatgcga gacggacgac ggctcctcgt tctcagccct cgacgtcatc cccaagggca
    50581 acgggacggg gctggcgccc tcgctgctga cggatgacac gacgatggtt tgctacaacg
    50641 gctgcgcctt cgaggcggac gccgacggga cgacatggac ggcggacatc cactcgtcgg
    50701 atgcgttcat caacaacctc atcgcgaacg gcaccggcag tttcaacggt gccgtcgaca
    50761 tcggcggcaa cctcttcgtc ggcggcggac cgacgaagtt cacggtccag gcaacgaccg
    50821 gaaacatcac gacgctcggc aattccatcc tcggcaactc ggactccgac acgacaagca
    50881 tcttcggtgc tggatacagc ttcggcctca cgggaacgac gctcactggt gcgcgcaacc
    50941 ttcggctcta cgacaccacg gcaacggctg cgaacgtcgg cgccgccatc tcgctcggct
    51001 acgacgcggg cggtggcact ggttaccagg accccgtaat catcaaggcc gtcaaggaga
    51061 acgggacggc ggcgaaccga gacactgcgc tcgtcttcgc cacggccacg gaggcgggcg
    51121 tcgggactcc caccgagcgc atgcgcatcc gcagcgacgg cgggatgacg ttcaacggca
    51181 cgacgggctc cgacggctac gtcctgacgg tcgatccgac gacacatatc ccgaagtggg
    51241 cggcagcgag tggcgccacg tattcggccg cgactccgct cacgctcacg ggctcgacgt
    51301 tcgacctcgc gtttacgtcg gactttcaag acagcgcagg gtctctcgac ctgtcgacgg
    51361 cggtcacagc gcccggctcg ctgacggtag cgacaacgct cggtgtgacc gggttgtcga
    51421 cgctcaccgg cggcgtcacg acgcctgcga gcctgaccac gacgggcacc ggcgacctcg
    51481 tctcaggtga cgacattacg tccgtcgacg acgtgaccgt gggcgacgcg ctcgcggtcg
    51541 tcggcgccag cgacctccgc ggcgccatct cgaacagcac cggcacgctg acgctcagcg
    51601 acagcgccgt cgacttcggc gccgattcct cgtcggtcgt caccgcgtca aacggaatcg
    51661 ttcacgcgct caactggtac ggcggcaaac actacgagtg gaacgacgaa tacatggatt
    51721 cgtcctactc ctcgtttggc ttcggggcgc tcggttgggt cacgaaccca acaggcacgg
    51781 gcgccgcgat ggtttcgtat accgggacgg ctgtctctgg tcgacccggc attgctcaga
    51841 tgtcaacggg gaccacgacg acaggctact cgaacgccta cacatcgctc ggcgccggag
    51901 cggcggctgg ctccatgtac gtcaccgaag gaacgtgggc attcgaggcc accggtcagt
    51961 tcgtcaacct ctcgacatca tctgaggagt actggtcaga gattgggttc ctcgacaacc
    52021 caaacgccgc cggcaacggc gacggctgct tccttctcta cgaccgtgcg aacacattct
    52081 ccggtgttgg tggcgtgaac accggcaacg ccgacaaatg gcaagcctgc acgaacaacg
    52141 gcgggacgcg ttcgtgcgtc ttgctcaacg gcacatctca gaacggcagc ggcggcgctg
    52201 gatcgatcac caccgtcgac acgacactga cggccgcatc gtggtggcgc gccaagatcg
    52261 tggcgcagac aggatcgtgc gcgttctcga tcgataccgg ctcgggcatg accctccgcg
    52321 cgacgatgac catcaacatc ccggcgacgg cgacggcgat cgcaatgaag atcgcaaaga
    52381 ccgtgggtac aacggcgcgg atgctgctga tcgatcagac ggcggtgtca ctcgacctga
    52441 ccaacgtcag gagcccgtga tgcgcctgtt cctcctcctg gcgctcgtcg cctgccgtct
    52501 gccccacgtc gagccggcgc tccgctcttc gcacgaccag cgccaggcgc tcgcggcgat
    52561 cacagtctcg tgcacctcac cggacccgtt cgccggcagc cctgacgtac cgaatgaccc
    52621 gacgcccgag gcgttcccca tcgagtggaa cgtcccgcat caggccaccg gcgtcgtcat
    52681 cagcgatcgc cacgtcctga ccgctgcaca cgccgtcatg tgcggcgcca tcccgcaagc
    52741 ggacgtgcag ctcgcgaacg gggcgcatca cgtcgtcgtc gagcgcgatg accgcatgtt
    52801 cccgcaccgc agcgaggaga ccgacgtcgc gcgcctcgag ctgttccacg cgggccgctt
    52861 cgacctcggc atccgtccgc ccgtcatccg cgacgcacgc ggtggcgagc cctgctgcgc
    52921 cgagacgctg cgcggcgaga cctgcgggcg catcgacccg tatcacctcg gctacgccct
    52981 cggcctcgac atccgcccag gtgactccgg tgcgccgctg tactgcgatg gcgcgctcgt
    53041 cgggctcgtc acgacggatg ccggccgctt cgaacctctg accccgtatt ggatggaagg
    53101 aacgtgagct tacagcgtca catctcgcgc gttagtacgg cgaagctcga cgctcttacc
    53161 gtcgcgcagc tcgtacacga cgagccacgt ctcgccatcg gcgtcggtct cccaggtgtg
    53221 caactgggcg ggcagtggcg tcgcgttgat cttcagccga acccaaccgt cgtttccgtc
    53281 gctcacgctg cgaccctacc acgcaactag gagacccatg cgcactctga ttctcgtctt
    53341 cgctctcatc gcctctgcct gcgtccaccc tcaagcgccc gcggcgccga agctggcgcc
    53401 cgagaatcaa gccgtcacct acgcgacgaa gaaggtcacg cccgatgccg cgtgcctgcc
    53461 gctgtcgacg gcgcagagcg gcagggccga ctcggcgttc tgcgaccttg gacgcgtcct
    53521 cgcgtactgc acgtctggac tcggcgccga tggactgtgc gttgccctgg ccgacatgcg
    53581 gccgaaggcg cccgaggcac cgaagacgga gccgaagcct gcggagcagc cggcaccgcc
    53641 cgtgcccgcg gcgcctcccc agcaagacgc gaagccgagc acgccgagca cgccgataaa
    53701 catgcgccga agcggtaagc gatgaagctg aacgaagctg tcgcgatcgc gcgccgcatc
    53761 ggtccgattc ggtgcgtcga cagcgacggc atcccggtga cgtccggttt tgggacagcc
    53821 ggcgccgtcg agatcagcgt caacgatggc gcgtttatag cggcgctcgg atcgatcacc
    53881 gtgatcggcg gcggctatgt ctattacaac gcgacaccgg cggatgcgtc tgttcgtgga
    53941 ttcgtttcag tcaaactcga gggcatctgc caggaatcga cattccgcga ggaagtggag
    54001 cggtccccac agggcatccc ggccggcacg gcagacgcag cgctctgccg ggttggtcca
    54061 ctgcgcatcg ttgatgccga cggagtggag ttagcatcgc tcgccggcat tacgtgcgag
    54121 atcagtatca acggatcggc gtgggcggcg ccaagcggcc tgctcgtgct gaccgaggcc
    54181 ggatatgcgg attacgtcgc ggcgctctct gagaccgcga gcgtagggtg gcttgccgta
    54241 aagctgaccg gcgcctgtca agagtttgcg atgcgcgcca cggtcgtgga tgcgaccacg
    54301 ggcggcggat cgttgtcagc cccgacgctc accgtgctga ccacattctc ggcgagcttc
    54361 gcggcagcgc gcctgacgcc atggcaggcc acgcttgatg attttccggt tggggcgcag
    54421 gcgatcatcc tcgtgcactt cgttgagcga aacgaaatgt acgtagctcg agacgccgag
    54481 ggcgtctggc gctggccgtt cgatctggaa cccgacaacg ccgtcgatct caatgtcgat
    54541 cccgtgaccg tgcagctcat accgcgcggt ggatggcatc cgtgcgacat cgaagtccag
    54601 atcgcggctg cggtcatggc cgaggaggct tagatgcccg gtccatcatt caccatcccc
    54661 gagccgccga gcgggtatca gcagacgcca gcgagcgagc aagctacgca ggacgcgcgg
    54721 ttctacggcg ccgatatctg gctcaatgtg gccgcacccg acgtgcagct cggccaggct
    54781 cgctacgtca ccacggcggc gggcgattgg gttcttgtgc gagggcgcga ggcgctgcgg
    54841 cagtcgctca tgcgacgact gatcacgaac ccgggcgact ggcagacgaa accagactac
    54901 ggcgttggcg cgcgtcagta cgtcaaggcg aagaacacgc gcaccgtgcg cgcagagcta
    54961 gagagccgca ttcgagcgca gttctcgcgc gatgtacgcg tgcatagcgt cgatctcgtg
    55021 acgctgacgc ggctcgacgg cgacaagccc gggatcaaga tctcggtgac cgtcacaccg
    55081 cgcggccgac tgcgcacgga tcagcccctc gaggtttctt tggaggtcac ttagccatgc
    55141 ccgttgctcc ctcattcgat gatctgatcg cgcagttcga ggccgaggca ctcgcacaaa
    55201 acagcgagct tgcgttcaac gacggcgatg tgaccgaggc gcaggctcac ggcgcgggcg
    55261 ccatggccga cgcggcgatc cgcttcaacg tgcaggcgtt caaggagacg tttatcgacg
    55321 gcgccaaggg cgacgctctg acggcgctcg tcgacgacca cctcaacatc cagcggcagc
    55381 cggcgacggc atcacagtgc accgttcggt tctctcgcac gagcggcggc gcaggattca
    55441 cgctcgccgc gggtttcgtg gtcgcgtcag aattcgacga cgccggcaat acgatcctgt
    55501 acacgctcga tgctgacgtc acgttcgcgg gcgcggacaa cgggccgcac tctggcagcg
    55561 tcacggccga gatcactggc aaggacggca acgtcgcggc agcgaagatc acgcggttcg
    55621 tggacgccaa gccagacgct acaatcgtag tcacgaacct ggcggcagct gccggcggca
    55681 acaacgagga atcggacagt gagctgcgcg tccgcgcgcg tctgttctgg cagacgttgc
    55741 gacgcggcac gctcgccgct ctagagttcg gcgcgctgca ggttgcaagc gttcgcaccg
    55801 tgcgcgcgac tgaggacatc agcacgggtg cggttaccgt cgtcgtggct gacagcgacg
    55861 gcgccagcac ggcgcaaatg gtgagcgatg cagaggccga gatcgaaaac tggcgcgctg
    55921 caggcgtgtc cgttgcggtt ctcggcggca cacagctgct cgtcaacgtg acgggcgtgc
    55981 tcgtcgtcaa cgatggcgtc gacgccgcgg cgctcgtgcc gctcgtgcag gccgcgatcg
    56041 agtcgcgtct cgccaagcag cggcagggag agctgctcta tctcgacagc gtcaaggctg
    56101 ccggcatcgg cgtggatccc gacgcgatcg acgcgctgac gctctcgctc ccagccgcga
    56161 acgtcacacc gacggccacg cagacggtgc gcgcaggcac gatcacgatc acataggagc
    56221 tgcgccatgg cactgacagc tgacgagcag aagttctacg atttcgcgaa ggcgtcgctg
    56281 ccggattgga tcccggacgg cgacgagttc ctcatgggcg cggccaaaat gttcggcagc
    56341 gtcaaggcgc tgctcgacta cctgttcggc cagacgctga tcggctcggc gatcggcggc
    56401 accgcaacca cgcccgactg gctcgatcag cacgcacgcg atcgcggcac tgcgcgccaa
    56461 gacggcgagg acgatccaac gctgcgcgat cgcctgcgca acgcacctga cgcgctcaca
    56521 cgcgcgagca ttctcgcagc cgtcaacgcg accctactgg ccaacgggat caccgatcct
    56581 gcggggctcg tggagctacc aaagca
//
LOCUS       DATLBZ010000073        18763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5277696, whole genome shotgun sequence.
ACCESSION   DATLBZ010000073 DATLBZ010000000
VERSION     DATLBZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18763
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5277696"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VMZ53_03915"
     CDS             <1..577
                     /locus_tag="VMZ53_03915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HUS27622.1"
                     /translation="DNLEINQVDAMKYDLRMLHRMLGEPLTVVGNLPYHLASQLIFKV
                     IDARDIVRFAVFMVQKEMADRILAKPATKEYGALGVMVRTYCDVKKVLDVGAGSFVPA
                     PKIDSTVIKLLPLANAEPRVPIANHPHYSKVVHAAFGQRRKTLRNALRAIWDEADVDA
                     ALAATKIDGIRRGETLDISEFGQLAAALPVR"
     gene            586..1410
                     /gene="mutM"
                     /locus_tag="VMZ53_03920"
     CDS             586..1410
                     /gene="mutM"
                     /locus_tag="VMZ53_03920"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HUS27623.1"
                     /translation="MPELPEVETVRRSLAPVVGARIVGVWDSAKGLHMRRKPPRARLK
                     KLVGATITEVRRHGKYLLVDTDGPLTLLIHLGMTGRVLIQQRSAPRPNHTHVVLDLGA
                     RELRFADARRFGQIDVLERSKETKHAALADLGPDPIVHGIDVDAFAARAKGKKATLKA
                     FLLDQTVLAGVGNIYASEALWRAQLKPTTRAHRLTAVTAAKLAAAIDETLRNALDNGG
                     TTFSDFIAADGAEGYNEDYLWVYGRAGEPCLRCKTLIKRAVHQGRVTYYCPTCQAR"
     gene            1491..2093
                     /locus_tag="VMZ53_03925"
     CDS             1491..2093
                     /locus_tag="VMZ53_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS27624.1"
                     /translation="MFNDTADEDLMVMYQKGEVRAFEVLLSRHRKPVFNFILRFVGDR
                     ETAEDLLQEAFMRVIKGAEAYKRQAKFTTWLYTIARNLCVDQTRRRKHRKHASLDAPM
                     DTSEESGTLMDVIPGNEMASDRKSVNKQLHEQMQRAIAGLSEEQREVFLMREFLDMPF
                     KQIADVVGVPENTVKSRMRYALEKLRLELDEYKDLAKAAP"
     gene            2090..2887
                     /locus_tag="VMZ53_03930"
     CDS             2090..2887
                     /locus_tag="VMZ53_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27625.1"
                     /translation="MMSHGDDDDGGDDAQLRSLRAVWLAMPDEDPPERGLDALMAAAH
                     TKAKQMADASSEMPAQAAAEPAIMTSWWERFVALFRRPPMLALATVIVLVAGAVVINN
                     RGMNATAPSTNSAPSPEDVPTERRERESLKDNAPPAMPEASGGAQEQDVRGPRRGTNE
                     GIVKEQATADKGEASNGRAGDSWVGVPLEEKTAEQASKDRLLDDAKTAAARGDCTSAR
                     NVARQLEKQDLGYYRSRVAPDPSIADCLQTRATGGTATDPATTTPSD"
     gene            complement(2893..3609)
                     /locus_tag="VMZ53_03935"
     CDS             complement(2893..3609)
                     /locus_tag="VMZ53_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27626.1"
                     /translation="MAKQKKPLFPNRLTLGIDEAGRGPAIGPMVMAAVALDSKAAAAL
                     TRKGLCDSKTYGAGEDAHQIRSELAAEVRTRATFVMSVEIEHTEIDLRVMKNELNALE
                     REHAAKLIEQAPICDKIIADGKRMFSQLTLRYPAFISCDGAESVHASVAAASVIAKVI
                     RDNRFNQIKQRYEADFGPITGGGYANAATRKWVRAYVEKHGKLPDEARLSWPYPFVAD
                     LLGTVRPLTSQIDMFAPPAV"
     gene            complement(3641..5833)
                     /locus_tag="VMZ53_03940"
     CDS             complement(3641..5833)
                     /locus_tag="VMZ53_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HUS27627.1"
                     /translation="MAKARAIAEAAPTALGRALRLDIVVAQGDASLAESQAQRAHDFY
                     LEALGLEPRNAELHGKVAAAHRSLGNLDAALTSYDRAVALGGDGGIEILRAAIDTAIA
                     ANDSTRAMQWANDLLGKDPTDVRALVARALAMMSAQPDAARALLELAAARDDVDAHVL
                     LARLSLPGDPSGAAKCALAALRTAPKNERARDILSQARAAELPVPASTEIADVAAFLE
                     KIVASKRELGHFVGEVARAAANLDQPLLVTVMGEFSSGKSSFVNAFIGADVAPTGITP
                     TTATINVVRYGRERGGRIIHHDGTTVELGWDPLMQSLRSLTPDDARSIDRVEILVPLP
                     HLEKINIVDTPGLNSIQPEHEATARAFIAKADAVVWVFTAAQGGKQSEKKALKSISDE
                     GKRVLGVLNKADQLSSSEITEVVDFIGGTLQDLVETIVPFSARHALTWKREGSGDDGN
                     WNALANALEERFFQQARQLKRDACARTLRAVVGEAQTVLARSRHAQVEAADAASAARD
                     ELIASARQFSGDGVIGERKALSEQMATLYRRAAREVLDLVRPRRLPFSSHTATAADRD
                     YLIALLSSGFETAIETGRRRVANELTSRSKTAEVAARSLEAALNYDVVGDLERVASDR
                     IGLAMSRVFDRARAYLRGYLEGGYVETFFRNDVPRLELAEDAIYHALYRSAPDLDREV
                     GDPLAHAATDALTALAKRLDHWRAVVDVRMFDLEVGVVRALEIVASRL"
     gene            5912..6481
                     /locus_tag="VMZ53_03945"
     CDS             5912..6481
                     /locus_tag="VMZ53_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27628.1"
                     /translation="MSGRCSFEVTERFVVRAAVGDERFAERGARIHIIAIFDDGLAQL
                     ANRFVEIAAMPPRETETAICVRQDRSVAASFRNRARQRHDRELERADLDERESMTDKR
                     CWIAWTELERALERRHRSLRLLNGQPGFAEDDQCLPVVRLHREQRFERRYRRARVALR
                     EQLARLVQLVPVFIEDQLTGEVAEPALEP"
     gene            6546..7301
                     /locus_tag="VMZ53_03950"
     CDS             6546..7301
                     /locus_tag="VMZ53_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27629.1"
                     /translation="MRRGWFAFVLLVGCGDVNKAAPDAPAPDAYEPDAPMACTGGQML
                     CMGGCEDVMTSTAHCGSCDKQCGALERCAAGTCKPFDSCADYKAVNAMLANGIYQSTA
                     KVDSFYCDFTNGQQYDFTLHDFTMMPEADYALMRATDFANTTTAAAFIALYNQRGGMR
                     PYKAWTSGNCCWSTTGGVRLAWNGSIMFSFQNGSSACGAYNPASVWQFGQGVSTAAPL
                     PLPANFFTTNTITETAGCGDANNPGIWMRKKAI"
     gene            complement(7314..8348)
                     /locus_tag="VMZ53_03955"
     CDS             complement(7314..8348)
                     /locus_tag="VMZ53_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27630.1"
                     /translation="MQRFLAISLLAATTTATAACGASDPADDGYGYGEGLGTPENPVP
                     QAEDKGPYAVTSRVDFTVEAVLPAQAELVVVTLREFSKNPAGGLIAAADAAGVPAVKA
                     LYDAIPGVIKDKLEGWINGEISKIKINGKTLPEYAGEMAALAETALTQFDLNSTLTMK
                     PGEATHTLTGIDFGAAGIDVTIPINGLAADILTQHPTITVAEGGSLAFGDQRFGLNFG
                     DYAWDSINQVSTTAFGGDIRSVLGKAMNCPALAHTISSKCVLNVCVGHETELKQICEG
                     GLDQIVDAVHAKFVAYDIDAFRFKTGAARLVDDDQDGVADRIVDGVWDGELNIGMGLR
                     HAPATFTATR"
     gene            complement(8480..10312)
                     /locus_tag="VMZ53_03960"
     CDS             complement(8480..10312)
                     /locus_tag="VMZ53_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HUS27631.1"
                     /translation="MILLEHHKQAKREIITRYKQLADVAESVGMVTLANDIRTTRIPK
                     IESEKFHLVVLGEFNHGKSTFVNALLGQDVLPTGITPTTASINHVLYADQANARVVLT
                     TGESKPLDPSQLKEWVTVAGGHADEVAYVELGYPSDMLRNSVVLVDTPGVNDLNEQRA
                     EVTYGYVPRADAVVFLLDAGQALKDSELEFLRSRVLESARDRLIFVLGKMDMLSPDER
                     TAVVEYVKQGLSKITHDPVLFPLSARDWSKSKDPASGMPELMAYFDRFLAHDRAQVIL
                     DNAAADAARTASYLENNLGVRMRAYDLDIGELEQRILAVREQLDASKRKLDELHVRIA
                     ADANSIKNQIELDLEAFAKAFVQALPFQIDAVDADDVKAYLPAFIEDKFKEWAEVEGA
                     KLAAMLEHLAEEVIQITNENVAAAAATLAERLGPNDTQVDITVDSFKYDVGIYAVGAL
                     GTTVMLFVNALAGGLLTLAAPILAIVLKSKIAGDIRIQAKEKVPAAVLKAAEAMKPHF
                     DRCIDDFAKRLSEFVFNAGNTLYKGISEILDRTMAERRERAGEIGDLRVATTTQIAQV
                     KATSSALRQLREGIWAGDDDAPAGGGPKPGSAAHSRETEDNGGN"
     gene            complement(10325..11215)
                     /locus_tag="VMZ53_03965"
     CDS             complement(10325..11215)
                     /locus_tag="VMZ53_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HUS27632.1"
                     /translation="MSVGVDFDFQRWIAQRRGQMEQSAREGAAYAYSGERKFRRTLMM
                     ARPVTMALEATTRLWRDVARTELLGTAVKVTDQQYPRVYEAAKAAATSLHVRMPAVFA
                     SPTSAFKVKILGTDDAPHLIVNLELAEKLDDTELLAAIAHELAHVQNGHILYTTALHY
                     LNTSAAFFVRWVIQPAIMTLQAWARRADVTCDRASLLVVRDVDKTLQALVRMELGLEK
                     GSAFNAEEYLRALPDAKKGGIGRFAEMFRSHPYVPKRVQALRLFSESALYALVTGQDT
                     AGKPSLVDIDKKVAELISVF"
     gene            complement(11232..12170)
                     /locus_tag="VMZ53_03970"
     CDS             complement(11232..12170)
                     /locus_tag="VMZ53_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HUS27633.1"
                     /translation="MGRRWIWLIVAAFIGLAAFSLVGDLDHLGDQLEGFGWPAFGAAL
                     ALALTNYLLRFVRWQLYLRRQDVRVPVASSFYVFMAGLSLSITPAKLGELLKSFLLRE
                     MHDVPAPRTAPIVVAERVSDLIALLVLAVIGVAAYGVDPTFVIAAGALIGIGLLLLAW
                     QAPTRALIDLATRPARVRRLREPLHETLRGLGTLCRPVPLLVSTAIAVPAWGCECLGF
                     KLICDAFPGAHTSLGSATLIYSVTTIAGALSFLPGGLGVTEGAMTVALVHEHIDKATA
                     VAATLLTRLATLWFAVLLGVVFLAIARRRIARRAAT"
     gene            12225..12599
                     /locus_tag="VMZ53_03975"
     CDS             12225..12599
                     /locus_tag="VMZ53_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS27634.1"
                     /translation="MTKSASILVVDDDPEIVTMLSTRLTKRGYKVSTASDGHKALELA
                     KRELPDVVLLDVMMPGKSGWEVARALKQDPVTQNVKIVMVSAIGEKTNEITAPIYGAD
                     AHVDKPFEFEALERVIANLLSA"
     gene            complement(12596..14821)
                     /locus_tag="VMZ53_03980"
     CDS             complement(12596..14821)
                     /locus_tag="VMZ53_03980"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS27635.1"
                     /translation="MPKAKPYPNGDPGTDTGDEEEKTTIESQWEDEASTTVEQGEVAE
                     KVRALKSNGNTNVTHTGAVEEPTVDDQHAPVLSAITPLRSDQNARLAITQGNDTGQTF
                     EIRPGKSYSIGRAIDNDIVLTDIAVSRRHFELRHDDGAWVIVDHRSGNGTVVNGNVED
                     NPFMLANGDVIEIGNTVFRFEQEKGPARPQQMPRMDEDEEELSTVAGKPLRAEIVEEP
                     MPVPVAPPRPQPQSRPKTLPPPAPLRAIGASQPPPLGLPPLAPQPASTLPLPQMANRP
                     PANAAPTMLGDSMGMPMNGMMMPGQLPMNAMGQPMPPTTIPGQGAPPRQSYPYPTTND
                     IPPHSVHAQMLLIQTQNRRGDASTAHVPPMSYDPMFGAPPMQQQPQRYAQPAISSRAK
                     YFVAAAAIAVIAAIATIAITKSGSKPKTVENRGVTGSASTKTGEPKKPVTVQSIEAPK
                     GTVAVTPTPTPTPTPTPTPAPPPKQEPPKQEPPKQVAITPPPKQEPPKQELPKQEPPK
                     QEPPKQVATTPPKKAEKAEKKVAARPPKKEEPKKEPVVAPKKVAVAVDTSSARSKADE
                     LYKKRKFSDAASTLTAAAKNADDNEAKELRATADYYTKLGKALNKGEAPAEDAIVAFE
                     NLRRAANYDSNVGNFYKAEIDSKLGSAASKAAFKYLANKDWQNARTAVISAEGHGAGD
                     SVGTVKTKLESVAREMYQQAQSDNFASQNSKDILNTLMKIVDSKSTWYQKAAKALAAN
                     R"
     gene            14945..15559
                     /locus_tag="VMZ53_03985"
     CDS             14945..15559
                     /locus_tag="VMZ53_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUS27636.1"
                     /translation="MQRPSKIIGIGQNYRAHAAEMGKGIPEEPLMFMKPPSAIIADGM
                     PIERPAGYERVDYEGELGVVIGKRAHRVPREQALEFVEGFTIVNDVTVRDLQKKDGQW
                     TRAKGFDTFCPIGPRVVPGLDPSKLHVTTRVNGVVKQDSPTSDLIFDVPTLIAFVTQH
                     MTLEPGDVISTGTPSGVGNLVPGDVVEVEISGIGVLRNPVIARP"
     gene            15556..16074
                     /gene="folK"
                     /locus_tag="VMZ53_03990"
     CDS             15556..16074
                     /gene="folK"
                     /locus_tag="VMZ53_03990"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HUS27637.1"
                     /translation="MKETTLVIGLGGNIGDEDAITGRFDHARAALLELGLTSKSAKLY
                     RTAAIGQPGAASQSDYLNTAVRIHVDEGVTGRQLYMTVREIEILLGRDRRSETRWGPR
                     TIDLDVILWGTRTFQTPELEVPHPRWSERLFVVRPLIDLFGEDAVIHGHRLGALEQQL
                     AAQRVQLVSARW"
     gene            complement(16058..16621)
                     /gene="plsY"
                     /locus_tag="VMZ53_03995"
     CDS             complement(16058..16621)
                     /gene="plsY"
                     /locus_tag="VMZ53_03995"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HUS27638.1"
                     /translation="MNLLVLVAGFFAGAIPFGVIVAKRRGVDIRKQGSGNIGATNVTR
                     VMGLSAGLLVLVLDALKGAAAVMLATRYSGMDPIIAMAGFAAILGHCFSPFLNGKGGK
                     GVATALGVFAVVAPALVFVAVGVFLVVAGRWRIPALGSLAGVTSAAVYSLATRQPMDV
                     TLLVLATLVLLVYTHRGNLQRLITSAR"
     gene            complement(16635..18584)
                     /locus_tag="VMZ53_04000"
     CDS             complement(16635..18584)
                     /locus_tag="VMZ53_04000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HUS27639.1"
                     /translation="MTELLAPGGGLERAIPYYEDRVEQRTMSAAVARALEDQRPLIVE
                     AGTGTGKTLAYLVPALQSGKRVVVSTGTRALQDQIARYDIPLLRMILGRPFNAVVLKG
                     VANYVCRRRLGEALSRGASEELVALREWSELSETGDRSEVEWLAESAPVWTDVTTTPD
                     ARIGPRCPYFERCFVTQARRLAEKAELILVNHHLYFADRALRAAHPGARVLPEHDAVI
                     FDEAHQLEDVATEHFGARVSTHKLSQLVRDAHLSLSRMPLWTGRAADDTIAAVERAGL
                     ALFSHIRGAIAGEADRVLLPEGLFEHPERQTAWFKLDTALEELARTAEAESEPPPDEE
                     PNEDAGARATLTGLARRARELRDDLATIAEQRARSHVYWGETRPTGTALTASPIEVGE
                     LVRRHIVMSGPTPIFTSATLAAAGDFSYQRARLGLGADADELLVPSPFDYATQAMLYV
                     PRDLPVPADDGFSAAAVQRTLELLALTSGRAFLLFTSHRALRDAATRLKDAPYARFVQ
                     GDAPRTTLIERFRSTPNAVLLGTASFWEGVDVPGDALSLVVIDKLPFAPHTDPLVAAR
                     MQQCVEKNGDPFNTIQLPAAAIALKQGFGRLIRRRDDRGIVAILDARIVTKTYGRVFL
                     DTLPSGLPRTSAIEQVRRWWAAPQS"
     gene            complement(18581..>18763)
                     /locus_tag="VMZ53_04005"
     CDS             complement(18581..>18763)
                     /locus_tag="VMZ53_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27640.1"
                     /translation="ELPSGPDDKVIYETADLVFHVMVGLTARRIPIQRVLDELARRFG
                     TSGHTEKASRPPKTPA"
BASE COUNT         3141 a         6040 c         6310 g         3272 t
ORIGIN      
        1 cgacaacctc gagatcaacc aagtcgacgc gatgaagtac gacctgcgca tgctccaccg
       61 catgctcggc gagccgctca ccgtcgtcgg gaatctgccg tatcacctcg catcgcagct
      121 catcttcaaa gtgatcgacg cgcgcgacat cgtgcgattc gccgtgttca tggtgcagaa
      181 ggagatggcc gaccgcatcc tcgccaagcc cgcgacgaaa gaatacggcg cccttggcgt
      241 gatggtgcgg acctactgcg acgtgaagaa ggtgctcgac gtcggtgcag gctcgttcgt
      301 gcccgcgccg aagatcgact cgacggtgat caagctgcta ccgctggcga acgccgagcc
      361 tcgcgtcccg atcgcgaatc acccgcatta ctcgaaggtc gtgcacgcgg cgttcggcca
      421 gcgtcgcaag acactccgca acgcgctccg cgcaatctgg gatgaagcag acgtcgacgc
      481 tgcgctcgcc gctacgaaga tcgatggcat ccgccgcggc gagacactcg acatctcgga
      541 gttcgggcag ctagcggccg cgttgccagt gcggtaagcg aagacgtgcc ggagctgccc
      601 gaggtggaga ccgttcgtcg atcgctggcg cctgtcgtcg gcgcgaggat cgtcggcgtg
      661 tgggactccg cgaaggggct gcacatgcgg cgcaagccgc cgcgcgcgag gctgaagaag
      721 ctcgtcggcg cgacgatcac cgaagtgcga cgccacggga agtatctgct cgtcgatacc
      781 gacggcccgc tcacgctgtt gatccatctc ggcatgaccg gccgcgtgct gatccagcag
      841 cgctctgcgc cgcgtccgaa ccacacgcat gtcgtcctcg atctcggcgc acgcgagctg
      901 cggtttgcgg acgcgcgccg gttcggccag atcgacgtgc tcgaacggag caaggagacc
      961 aagcatgcgg cgctcgccga cctcggcccc gatcccatcg tgcacggcat cgatgtcgac
     1021 gcattcgcag cgcgcgcgaa gggaaagaaa gcgacgctca aagcgttcct tctcgatcag
     1081 acggtgcttg cgggcgtcgg gaacatctac gcatccgaag cgctgtggcg tgcgcagcta
     1141 aagccgacca cgcgtgcgca tcggttgacc gccgtgaccg ccgccaagct cgctgccgcg
     1201 atcgacgaga cgctccgcaa tgcactcgat aacggcggca ccacgttctc cgacttcatc
     1261 gccgccgacg gcgctgaggg ctacaacgag gactatttgt gggtctacgg gcgtgccggt
     1321 gagccgtgcc tgcggtgcaa gacgttgatc aagcgtgccg tccaccaagg acgggtcacg
     1381 tattactgtc caacgtgcca ggcccgctga gggtctaatt caggcccttc cccgggctcg
     1441 tacgttcgtt gtgttagtgt gacccgggcc gggcgttcta cctgggcgca atgttcaacg
     1501 acactgccga cgaagacctg atggtcatgt accagaaggg cgaggttcgc gccttcgagg
     1561 tcttgctgtc gcggcatcgc aagcctgtct tcaacttcat cctgcgattc gtcggagatc
     1621 gcgagaccgc tgaggatctc ctgcaggaag ccttcatgcg cgtcatcaaa ggcgcggagg
     1681 cttacaagcg gcaggccaag ttcacgacgt ggctttacac catcgcgagg aacttgtgcg
     1741 ttgaccagac taggagacgc aaacatcgca agcacgcgtc gctggatgcc ccgatggaca
     1801 ccagtgaaga gagcggcacg ttgatggatg tgatccccgg caacgagatg gccagcgatc
     1861 gcaagagtgt gaacaagcag ctgcacgaac agatgcagcg cgcgatcgcc ggcctttcgg
     1921 aagagcagcg cgaagtgttc ctgatgcgcg agttcctcga catgccgttc aagcagatcg
     1981 cggatgtcgt cggcgtgccc gagaacacgg tgaagagccg catgcgctac gcgctcgaga
     2041 agctgcggct cgagctcgac gagtacaagg atctcgcgaa ggccgcgccg tgatgagcca
     2101 cggagacgac gacgacggcg gcgacgacgc gcagctccgg tcgttgcgcg cggtatggct
     2161 cgcgatgccc gacgaggatc cgccggagcg cggcctcgat gcactgatgg ctgctgcgca
     2221 taccaaggca aagcagatgg ccgacgcgtc gagcgagatg ccggcgcaag ctgcggccga
     2281 gcccgcgatc atgacgagct ggtgggagcg attcgtcgcc ctgttccgcc gcccaccgat
     2341 gctcgcgctc gcgacggtga tcgtgctcgt ggcgggggcc gtggtgatca acaaccgcgg
     2401 catgaacgcg accgcgccgt cgacgaattc agcgccgtct cccgaggacg tgccgaccga
     2461 aaggcgcgag cgcgagagcc tcaaggacaa cgcgccgccc gccatgccag aggcctccgg
     2521 tggtgcccag gagcaggacg tgcgcggccc gcgacgcggc acgaacgaag gcatcgtcaa
     2581 ggagcaggcg accgccgata agggcgaagc ctcgaacggg cgcgcagggg attcgtgggt
     2641 cggcgtaccg ctcgaggaga agacggcgga acaagcgtcg aaggatcggc tgctcgatga
     2701 cgcgaagacc gcggcagcac gcggtgactg cacgagtgct cgcaacgtcg cgcggcagct
     2761 cgagaagcag gacctcggct actaccgctc gcgcgtcgcg cccgacccga gcattgcgga
     2821 ttgtctgcag actcgcgcca cgggcggaac ggcgaccgat cccgcgacga cgactccgag
     2881 cgactagagc acctagacgg caggcggcgc gaacatgtcg atctgcgacg tgagcgggcg
     2941 aaccgtcccg agcaaatcgg cgacgaacgg ataaggccac gacaggcgcg cttcgtccgg
     3001 cagcttgccg tgcttctcga catacgcgcg gacccacttg cgcgtggccg cattcgcgta
     3061 gccgccgccg gtgatcggac cgaagtccgc ttcgtagcgc tgcttgatct gattgaaccg
     3121 gttatcgcgg atcacctttg cgatcaccga cgctgcggcg accgatgcat gcaccgactc
     3181 ggcgccgtcg cacgagatga atgcagggta gcggagcgtc agctgcgaga acatgcgctt
     3241 gccgtcggcg atgatcttgt cgcagatcgg cgcttgctcg atgagctttg cagcgtgctc
     3301 gcgctcgagc gcgttgagct cgttcttcat gacgcggaga tcgatctccg tatgctcgat
     3361 ctcgacggac atcacgaacg tcgcgcgcgt gcgtacctcg gcggcgagct cgctgcgaat
     3421 ctgatgagca tcctcaccag cgccgtacgt tttcgaatcg cagaggcctt tgcgcgtgag
     3481 cgccgccgcc gccttcgagt ccagggccac cgccgccatc accatcggac cgattgccgg
     3541 cccgcgacct gcctcgtcga tcccgagcgt cagccggttc gggaagagag gcttcttctg
     3601 tttggccatg atgcgcggag cctacgtggg aggtgcgaca tcataagcga gacgcgacga
     3661 tctcgagagc cctaacgaca ccgacctcga gatcgaacat ccggacgtcg accacggcgc
     3721 gccagtgatc gaggcgcttt gcgagggcgg tgagggcatc ggtggcggcg tgtgcgagag
     3781 gatcaccgac ctcacgatcg agatcgggag cggaacggta gagcgcgtga tagatcgcgt
     3841 cctcggcgag ctcgaggcga ggcacgtcgt tgcggaagaa cgtctcgaca tagccgcctt
     3901 cgaggtagcc acgcaggtag gcgcgcgcgc gatcgaagac gcgagacatc gcgaggccga
     3961 tgcgatcgga agcgacgcgc tcgagatccc cgacgacgtc gtagttgagc gcggcctcca
     4021 gcgagcgagc cgcaacctct gcagtcttgc tgcgcgacgt gagctcgttc gcgacacgac
     4081 gccggccagt ctcgatcgcg gtctcgaatc ccgacgacaa gagcgcgatg agatagtcac
     4141 ggtcggcggc ggtggcggta tgcgaggaga acggcagtcg acgcgggcgc acgagatcga
     4201 ggacctcgcg agcggcacgc cgatagagtg tagccatttg ctcggagaga gctttgcgct
     4261 caccgatgac accatcgccg gagaattggc gcgctgacgc gatgagctcg tcgcgtgcgg
     4321 cggatgcagc atcggcggcc tcgacttgcg catgccgtga acgcgcgagg acggtctgag
     4381 cttctccgac gacagcgcgc aacgtgcgag cacacgcatc gcgcttgagc tggcgcgctt
     4441 gctggaagaa gcgttcctcg agtgcattgg cgagcgcgtt ccagttgccg tcgtcgccgg
     4501 agccttcgcg cttccacgtc agcgcatggc gcgcggagaa cgggacgatt gtctcgacga
     4561 gatcctgcag cgtgccgccg atgaagtcga cgacctcggt gatctccgac gaagacagct
     4621 ggtcggcctt gttgagcacg ccgagcacgc gcttgccctc gtcgctgatc gacttcagcg
     4681 ccttcttctc gctctgcttg ccgccttgtg cggcggtgaa cacccatacg acggcatcgg
     4741 ctttcgcgat gaacgcgcgc gcggtcgcct cgtgctcggg ctggatcgag ttgaggccgg
     4801 gcgtgtcgac gatgttgatc ttttcgaggt gcggcagcgg aacgaggatc tcgacgcgat
     4861 cgatcgagcg tgcgtcgtcc ggcgtgaggc tgcgcaagct ctgcatcagc ggatcccagc
     4921 cgagctcgac ggtggtgccg tcatgatgaa tgatgcggcc gccgcgctcg cgtccatagc
     4981 gaacgacgtt gatcgtcgcg gtcgttggcg tgatgcccgt cggtgcgacg tcggcgccga
     5041 tgaatgcatt gacgaacgac gacttgccgc tcgagaactc gcccatcacg gtcacgagca
     5101 gtggctgatc gagattggcg gccgcacgcg caacctcgcc gacgaagtgg ccgagctcgc
     5161 gcttgctcgc gacgatcttc tcgaggaacg cggcgacatc cgcgatctcc gtcgaggctg
     5221 ggacgggaag ctcggccgcg cgtgcctgcg agaggatgtc ccgggctcgc tcgttcttcg
     5281 gggcggtgcg gagcgcagcg agtgcgcact tggcggcacc gctcggatcg ccgggcagcg
     5341 agaggcgggc gaggaggacg tgtgcgtcga cgtcgtcgcg agcggccgca agctcgagga
     5401 gagcacgcgc tgcgtccggc tgcgccgaca tcatggcaag tgcccgggca acgagtgcgc
     5461 ggacgtccgt agggtccttg ccgaggagat cgttcgccca ctgcatcgcg cgggtcgagt
     5521 cattcgcagc aatcgccgtg tcgatcgccg ctcgcaggat ctcgatgcca ccgtcgccgc
     5581 cgagcgcgac tgcgcggtcg tagctcgtca atgctgcgtc gaggttgccg agcgagcgat
     5641 gcgccgctgc gacctttccg tgcagctccg cattgcgggg ttcgaggccg agcgcttcga
     5701 ggtagaaatc gtgtgcacgc tgcgcctggc tctccgcgag tgatgcatcg ccttgcgcga
     5761 cgacgatatc gagccgcaga gcacgaccaa gtgcggtcgg cgccgcttcg gcaatggcgc
     5821 gggccttggc caaacgctca cccgccgtcg ccggggcgtc gtcgacgagt gccagtcgac
     5881 cgagagcgta aagtgcgagt gccggtggct catgagcggc cgctgctcgt tcgaggtgac
     5941 ggagcgcttc gtcgtacggg ccgctgtcgg cgacgagcgc ttcgccgagc gcggcgcgcg
     6001 catccatatc atcgccatct tcgacgacgg ccttgcgcag ctcgcgaatc gctttgtcga
     6061 gatcgccgcg atgcctccac gcgagaccga gaccgcgata tgcgtccgcc aagatcgctc
     6121 ggtcgccgcc agcttccgca accgcgcgcg acagcgccac gatcgcgagc tcgaacgcgc
     6181 cgatctcgac gagcgagagt ccatgaccga caagcgctgc tggatcgcct ggacggagct
     6241 tgagcgcgcg ctcgaacgcc gtcaccgcag cctgcggctc ctgaatggcc agccgggctt
     6301 cgccgaggac gatcaatgct tgcccgtggt caggcttcac cgagagcagc gcttcgagag
     6361 acgctatcgc cgcgctcgcg tcgccttgcg cgagcaactg gcgcgcctgg tgcaactggt
     6421 cccggtattc atcgaggacc agctcactgg cgaggtcgcc gagccggcgc tcgagccgtg
     6481 accaaagcga catgaacgca gcctaccatt cgatttgggc ggtggtttgc ccgtatgatg
     6541 gaggcatgcg aagagggtgg ttcgcgttcg tgctcctcgt cggctgcggc gatgtaaata
     6601 aggccgcccc ggatgcaccg gcgcctgacg cgtatgaacc ggatgcgcca atggcctgca
     6661 ccggcggcca aatgctctgc atgggcggct gcgaagacgt gatgacgagt acggcgcact
     6721 gcggaagctg cgacaagcag tgcggggcgc tcgagcgttg cgcggccggc acctgcaagc
     6781 cgttcgattc gtgcgccgat tacaaggcgg tcaacgcgat gctggcgaac ggcatctacc
     6841 agagcacggc aaaggtcgac agcttctact gcgacttcac gaacggccag cagtacgact
     6901 tcacgctgca cgacttcacg atgatgccgg aagccgatta cgcgctcatg cgagcgacgg
     6961 acttcgccaa cacgacaacc gcagcggcgt tcatcgcgct ctacaaccaa cgcggcggca
     7021 tgcggcccta caaggcgtgg acatcaggta actgctgctg gtccaccaca ggtggtgtgc
     7081 ggctcgcatg gaacggctcg atcatgttct cgttccagaa cggcagctcg gcctgcggcg
     7141 cgtacaatcc cgcgagtgtc tggcagttcg gtcaaggcgt gagcaccgct gcgccgttgc
     7201 cgcttcccgc gaacttcttc acgacgaaca cgatcaccga gactgctggg tgcggtgacg
     7261 ccaacaatcc cggcatctgg atgcgcaaga aggcgatctg atcgcctcac cgatcagcgc
     7321 gtcgccgtga acgtcgcggg cgcgtggcgc agacccatgc cgatgttcag ctcgccgtcc
     7381 cacacgccgt cgacgatgcg gtccgcgacg ccgtcctgat cgtcgtcgac gaggcgcgcc
     7441 gcgcccgtct tgaagcggaa cgcgtcgata tcgtacgcga cgaactttgc gtgcaccgcg
     7501 tcgacgatct gatcgagacc gccctcgcag atctgcttca gctcggtctc gtgcccgaca
     7561 cacacgttga ggacgcactt gctcgagatc gtgtgcgcga gcgcagggca gttcatcgcc
     7621 ttgccgagca cgctgcgaat atcgccgcca aacgcggtcg tgctgacctg attgatgctg
     7681 tcccacgcgt aatcgccgaa gttcagaccg aagcgctgat cgccaaacgc gagcgagccg
     7741 ccctccgcga cggtgatggt cgggtgctgc gtaaggatgt ccgccgccag tccgttgatc
     7801 ggaatcgtca cgtcgatgcc agcggcgccg aagtcgatgc cggtcaacgt gtgcgtcgct
     7861 tcgcccggct tcatcgtcag cgtgctgttc aagtcgaatt gcgtcagcgc ggtctcggcg
     7921 agcgccgcca tctcacccgc gtattcgggc agcgtcttgc cgttgatctt gatcttgctg
     7981 atctcgccgt tgatccaacc ttcgagcttg tctttgataa cccccggaat cgcgtcgtac
     8041 agagccttca ccgcgggcac gcccgccgca tccgccgccg cgatcaaacc gccggccggg
     8101 ttcttcgaga actcgcgcag cgtcacgacg acgagctccg cctgtgcagg cagcaccgct
     8161 tcgaccgtga agtccacgcg gctcgtcacc gcatacggtc ccttgtcctc ggcctgcggc
     8221 accggattct ccggcgtgcc gagcccttcg ccgtacccgt agccgtcgtc ggccgggtcg
     8281 gaagcaccgc acgctgcggt tgcggttgtg gtcgcggcga gaagcgagat ggcgagaaat
     8341 cgctgcatgg ccggctacaa cagcaagcgc gttgccagct ctaagtgcgc acgatcgctg
     8401 atgacgcacc gctcatcccg atgggagcgg actccacgtg cccacgcgcg atggcggcta
     8461 ctcgagtagc cgtggggcgc taattgccgc cgttgtcttc ggtctcgcga gagtgggcag
     8521 cgctgccggg cttcggtccg ccgcccgctg gcgcgtcatc gtcgccggcc cagattcctt
     8581 cgcggagctg gcgaagcgcc gacgacgtgg ctttgacttg ggcgatctgc gtcgtggtgg
     8641 cgacgcggag gtcaccgatc tcgccggcgc gttcgcggcg ttccgccatc gtccggtcga
     8701 ggatctcgct gatgcccttg tagagcgtgt tgcctgcgtt gaacacgaac tccgagagcc
     8761 gcttcgcgaa gtcgtcgatg cagcgatcga agtgcggctt catcgcttcg gcggctttga
     8821 ggacggccgc ggggaccttc tctttcgctt gaatgcggat gtcgccggcg atcttggact
     8881 tgagaacgat cgccaggatg ggcgcggcga gcgtgagcag gccacccgcg agcgcgttga
     8941 cgaacagcat caccgtcgtg ccgagcgcac ccaccgcgta gatgccgacg tcgtacttga
     9001 acgagtcgac cgtgatgtcg acctgcgtgt cgttcgggcc gagccgttcg gcgagcgtgg
     9061 ctgcggctgc cgcgacgttc tcgttcgtga tctggatgac ttcttcggcg aggtgctcga
     9121 gcatcgcggc gagcttggcg ccctcgactt ccgcccactc cttgaacttg tcttcgatga
     9181 aagcgggcag gtacgccttc acgtcgtcgg cgtcgaccgc atcgatctgg aaggggagcg
     9241 cttggacgaa cgcttttgcg aacgcttcga ggtcgagctc gatttggttc ttgatcgagt
     9301 tggcgtcggc tgcgatgcgg acgtgaagct cgtcgagctt gcgcttggat gcgtcgagct
     9361 gctcgcgtac agcgaggatg cgctgctcga gctcgccgat gtcgagatcg tacgcacgca
     9421 tgcgaacgcc gaggttgttc tcgaggtagc tcgcggtgcg cgcggcgtcg gcggctgcgt
     9481 tgtcgaggat gacttgcgcg cggtcgtgtg cgaggaagcg gtcgaagtac gccatcagct
     9541 ccggcatgcc cgaggccggg tctttcgact tcgaccaatc gcgcgcggag agcgggaaca
     9601 ggaccggatc gtgcgtgatc ttcgagaggc cttgtttgac gtactcgacg acggccgtgc
     9661 gctcgtccgg cgagagcatg tccatcttgc caagcacgaa gatcaggcga tcgcgcgcgg
     9721 actcgagcac gcggctgcga aggaactcga gctcgctgtc tttgagcgct tggcctgcgt
     9781 cgaggaggaa cacgactgca tcggcgcgcg gcacgtagcc gtacgtgact tcggcgcgtt
     9841 gctcgttgag atcgttgacg cctggggtgt cgacgaggac gaccgagttg cggagcatgt
     9901 cgctcgggta accgagctcg acgtacgcca cttcgtctgc gtggccaccg gcgaccgtca
     9961 cccactcttt gagctgcgat ggatcgagcg gcttggattc tcccgtcgtc agcacgacgc
    10021 gcgcgttggc ctgatcggcg tagagcacgt gattgatcga cgcggtcgtc ggcgtgatgc
    10081 ccgtcggcaa cacgtcttgg ccgagcagcg cattcacgaa cgtcgacttg ccgtggttga
    10141 actcgccgag cacgacgaga tggaacttct cgctctcgat cttcgggatg cgcgtcgttc
    10201 ggatgtcgtt cgcgagcgtg accatgccga ccgattcggc gacgtccgcg agctgcttgt
    10261 agcgagtgat gatctcgcgc ttggcttgct tgtgatgctc gagcaggatc atggcggcgc
    10321 ctccctagaa cacgctgatg agctcagcga cctttttgtc gatgtcgacg aggctgggtt
    10381 tgccggccgt gtcttgtccg gtgacgagtg cgtagagcgc agactcggag aataggcgga
    10441 gcgcctgcac gcgcttcggc acgtaggggt gcgagcggaa catctcggcg aaccgtccga
    10501 tgccgccctt cttcgcgtcg ggcaacgcgc ggagatactc ctcggcgttg aacgcggagc
    10561 ccttctcgag accgagctcc atgcggacga gcgcctgcag cgtcttgtcg acatcgcgca
    10621 cgacgaggag cgacgcgcga tcgcacgtga catccgcccg gcgcgcccac gcttgcagcg
    10681 tcatgatcgc gggctggatc acccagcgca cgaagaatgc ggccgacgtg ttcaggtagt
    10741 gcagcgccgt cgtgtagagg atgtggccgt tttggacgtg agcgagctcg tgcgcgatcg
    10801 cggcgagcag ctccgtgtcg tcgagcttct ctgccagctc gaggttgacg atcaggtgcg
    10861 gcgcgtcgtc ggtgccgagg atcttcactt tgaatgcgga cgtcggcgac gcgaacacgg
    10921 cgggcatccg cacgtgcagt gacgtcgctg ccgccttcgc cgcttcgtac acacgcgggt
    10981 actgctgatc ggtgaccttg acggccgtcc cgagcaactc ggtgcgcgcg acatcacgcc
    11041 agagacgcgt cgttgcttcg agcgccatcg tcacgggacg cgccatcatc agcgtgcggc
    11101 ggaacttgcg ctcgccgctg tacgcgtacg cagcgccttc cctcgccgac tgctccatct
    11161 gaccgcgccg ctgtgcgatc cagcgttgga aatcgaaatc gacgcctacc gacatggcga
    11221 tcttatagca ctcaggtcgc ggcccggcgg gcgatgcggc ggcgtgcaat cgccagaaag
    11281 accacaccga ggaggacggc aaaccagagc gtcgcgagcc gggtcagcaa cgtggccgca
    11341 actgctgtgg ctttgtcgat gtgctcgtgc acgagtgcga ccgtcatcgc gccctcggtc
    11401 acacctagcc caccgggcag gaacgacagg gcaccggcga ttgtcgtcac ggaataaatc
    11461 agcgtcgcgc tccccagcga tgtgtgagcg cccggaaacg cgtcgcagat gagcttgaag
    11521 ccgagacact cgcagcccca cgcgggtacc gcgatcgcgg tcgacacgag cagcggcact
    11581 gggcgacaca acgtgccgag ccctcgcagc gtttcgtgca gcggctcgcg aagccgacgc
    11641 acacgcgcgg gccgcgtagc gagatcgatg agcgcgcgcg tcggtgcctg ccatgcgagg
    11701 agcagcagcc cgatgccgat cagggctccc gccgcgatga cgaatgtcgg gtcgacgccg
    11761 tacgccgcga cgccgattac cgcgagcacg agcaatgcga tcagatcgct cactcgctcg
    11821 gccacgacga tcggcgcggt ccgtggagcg ggcacatcat gcatctcgcg caacaggaag
    11881 ctcttgagca gctcgccgag cttcgcggga gtgatcgata gcgagagccc ggccatgaac
    11941 acgtagaagc tcgatgcgac cggaacgcgg acgtcttgcc tgcggaggta gagctgccag
    12001 cgcacgaacc gcagcaggta gttcgtcaaa gcgagcgcga gcgccgcacc aaacgcgggc
    12061 cacccgaacc cttctagctg gtcgcccaga tggtcgaggt cgccgaccag tgagaacgca
    12121 gctaggccga tgaaggctgc aacgatcagc cagatccagc ggcgtcccac gtcgcttcct
    12181 atcacgcctt gacctcgcag agcgcaggcg cgtagcctcg gttcatgacg aagtcggcga
    12241 gcattctggt ggtggacgac gatcccgaga tcgtgaccat gctgagcacc cgactgacga
    12301 agcgcggcta caaagtctcg acggcgagcg atggccacaa ggcgctggag ctcgccaagc
    12361 gcgagctacc cgatgtcgtg ctcctcgacg tcatgatgcc gggcaagtcg ggctgggaag
    12421 tggcgcgcgc gctcaagcag gatcccgtca cgcagaacgt gaagatcgtg atggtgtcgg
    12481 cgatcggcga gaagacgaac gagatcacgg cgccgatcta cggagccgat gcgcacgtcg
    12541 acaagccgtt cgagttcgaa gcgctcgagc gggtgatcgc gaatctgctg agcgcctaac
    12601 ggttcgcggc gagcgccttc gccgccttct gataccaggt gctcttcgag tcgacgatct
    12661 tcatgagcgt gttcaggatg tccttgctgt tctggctcgc gaagttgtcc gactgcgcct
    12721 gctgatacat ctcgcgcgcg acggactcga gcttcgtctt cacggtgccg acgctgtccc
    12781 cagcgccgtg gccctcggct gagatcactg cggttcgcgc gttctgccag tccttgttcg
    12841 cgaggtactt gaacgcagcc ttcgacgcgg cggagccgag cttgctgtcg atctcggcct
    12901 tgtagaagtt gccgacgttg ctgtcgtagt tcgcggcgcg gcggaggttc tcgaacgcga
    12961 cgatcgcgtc ctctgcagga gcctcgccct tgttgagtgc cttgccgagc ttcgtgtagt
    13021 agtccgccgt cgcgcgcagc tctttcgctt cgttgtcatc cgcgttcttc gcggcagcgg
    13081 tcagcgtgct cgccgcgtcg ctgaacttcc gcttcttgta gagctcgtcc gccttcgagc
    13141 gcgccgacga cgtatccacc gcaaccgcga ccttcttcgg cgcgaccacg ggctccttct
    13201 tcggctcttc tttcttcggc gggcgcgcag cgaccttctt ctccgccttc tccgccttct
    13261 tcggcggcgt cgtcgcgacc tgcttgggcg gctcctgctt gggcggctcc tgcttgggca
    13321 gctcttgctt gggcggctct tgcttgggcg gcggcgtgat cgcgacctgc ttgggcggct
    13381 cttgcttggg cggctcctgc ttgggcggcg gcgccggcgt tggtgtcggc gtcggcgtcg
    13441 gcgtcggcgt gggcgtgacg gcgaccgtgc ccttcggagc ctcgatcgac tgcaccgtga
    13501 cgggcttctt gggctcgccc gtcttcgtgc tcgcgcttcc ggtcacgcca cggttctcga
    13561 cggtctttgg cttactgccc gacttcgtga tcgcgatcgt tgcgatcgcc gcgatgaccg
    13621 cgatggccgc cgcagcgacg aagtatttcg cgcgcgagct aatcgcaggc tgcgcgtagc
    13681 gctgcggctg ctgctgcatc ggcggcgcgc cgaacatcgg gtcgtacgac atcggcggca
    13741 cgtgcgccgt cgacgcgtcg cctcggcgat tctgcgtttg gatcagcagc atctgcgcgt
    13801 gcacggagtg cggcgggatg tcgttcgtcg tcggatacgg atacgactgc cgcggcggcg
    13861 cgccttggcc gggaatcgtc gtcggcggca tcggctgccc catcgcattc atggggagct
    13921 ggccgggcat catcatcccg ttcatcggca tacccatgga atcgccgagc atcgtcggtg
    13981 cggcgtttgc cggcgggcga ttcgccatct gcggcagcgg cagcgtcgat gcgggctgcg
    14041 gcgcgagtgg cggcaagccc agcggtggcg gttggctcgc cccgatcgcg cgcaagggcg
    14101 ccggtggcgg cagcgtcttc ggacgcgact gcggttgcgg acgcggcggt gcgaccggca
    14161 ccggcatcgg ctcttcgacg atctctgcgc gcagtggttt gccagcgacg gtcgagagct
    14221 cctcttcgtc ctcgtccatc cgcggcatct gctgcggacg tgcaggcccc ttctcctgct
    14281 cgaagcggaa gaccgtgttg ccgatctcga tgacatcgcc gttcgcgagc atgaacgggt
    14341 tgtcctcgac gttgccgttg acgaccgtgc cgttgccgct tcggtgatcg acgatgaccc
    14401 acgcgccgtc atcgtgtcgc agctcgaagt gtcgacgaga aaccgcgata tcggtgagca
    14461 cgatgtcgtt gtcgatcgcg cggccgatcg aataggactt gcccggccga atctcgaacg
    14521 tctgcccggt atcgttgccc tgcgtgatcg cgagtcgcgc attctgatcc gaacgcagcg
    14581 gcgtgatcgc ggagagaacc ggtgcgtgct gatcgtcgac cgtcggctct tcgaccgcgc
    14641 cggtatgcgt gacgttcgtg ttgccgttgc tcttgagcgc gcgaaccttt tcggcgactt
    14701 caccttgttc gacggtcgtg ctcgcttcgt cttcccactg cgattcgatc gtcgtctttt
    14761 cttcttcgtc gcctgtatcg gtgcccgggt cgccatttgg atatggcttc gccttcggca
    14821 caacgtgcgc acttacacgt tgtggccctc cagggttccc ggcgcggccc atcgaatgaa
    14881 attgtacggg gcgcaacgct atcgatcaat gacaccaccg acgaggtgtc ctacctttcc
    14941 cgccatgcag cgccccagca agatcatcgg catcggccag aactaccgag cgcacgcggc
    15001 ggagatgggc aaggggattc ccgaggagcc cctgatgttc atgaaaccgc cctccgcgat
    15061 catcgccgac ggcatgccga tcgagcgtcc tgcgggctac gaacgagtcg attacgaggg
    15121 cgagctgggc gtcgtgatcg gcaagcgcgc gcatcgcgtg ccccgcgaac aggccctcga
    15181 gttcgtcgaa ggcttcacga tcgtcaacga tgtgaccgtt cgcgacctcc agaaaaaaga
    15241 cggccagtgg acgcgcgcga aaggcttcga cacgttctgt ccgatcggcc cacgcgtcgt
    15301 gcccgggctc gatccatcca agctgcacgt caccacgcga gtgaatggcg tcgtgaagca
    15361 ggactcgccg acgagcgatc tgatcttcga cgtcccgacg ctgatcgcat tcgtcacaca
    15421 gcacatgacg ctcgaacccg gcgacgtgat ctcgaccggc acaccatcgg gcgttggcaa
    15481 cctcgttccg ggcgatgtcg tcgaagtcga gatcagcggc atcggcgtgc tgcgcaatcc
    15541 ggtgatcgca cggccgtgaa agagaccacg ctggtcatcg gcctcggtgg caacattggc
    15601 gacgaggacg cgatcaccgg tcgcttcgat cacgcgcgcg ctgcgttgct cgagctcggg
    15661 ctgacgtcga agtcggcgaa gctctatcga acggcggcga tcggtcagcc gggtgcagcg
    15721 tcgcagtcgg actatctcaa cactgcggtc cgcatccacg tcgacgaggg cgtgacaggg
    15781 cgacagctct acatgacggt gcgcgagatc gaaatcctgc tcggtcgcga ccggcgtagc
    15841 gaaacgcgat ggggcccgcg gacgatcgat ctcgacgtga tcctgtgggg cacgcgcacg
    15901 ttccagaccc cggagctcga ggtgccgcac ccgcggtgga gcgagcggct gttcgtcgtg
    15961 cggccgctga tcgacctgtt cggcgaggat gccgtcatcc acggccaccg gctcggcgcg
    16021 ctcgagcagc agctcgccgc tcagcgtgtg cagctcgtca gcgcgcgctg gtgatcagtc
    16081 gctgcaggtt gccgcgatgc gtgtacacga gcagcacgag tgtcgcgagc acgagtagcg
    16141 tgacgtccat cggctgccgc gttgccagcg agtagacggc ggcggacgtg acgccggcga
    16201 gcgagccgag ggctggaatg cgccagcgtc ccgcgacgac gaggaacacg ccgacggcga
    16261 cgaacacgag cgccggtgcg acgaccgcga acacgccgag tgcggttgcg acgcccttgc
    16321 cgcctttgcc attgaggaac ggcgagaagc agtggccgag gatcgcggcg aagccggcca
    16381 tcgcgatgat cgggtccatc cccgagtagc gcgtcgcgag catgacggct gctgcgccct
    16441 tcagcgcgtc gagcacgagc acgaggagac cggcactgag tcccatcacg cgcgtcacat
    16501 ttgtcgcgcc gatgttgccg ctgccttgct tgcggatgtc gacgcctcgg cgcttcgcga
    16561 cgatgactcc aaacgggatc gcacccgcaa agaatccggc gacgagcacg aggaggttca
    16621 cgctctcagt gtagctagct ctgcggagct gcccaccacc gacgaacttg ctcgatcgca
    16681 gatgtgcgcg gcagcccact cggcaacgta tcgaggaaca cgcgcccgta cgtttttgtc
    16741 acgatgcgcg catccaggat cgccacgatg ccgcggtcgt ctcgtcgacg aatcagccgg
    16801 ccgaagccct gcttgagcgc gatggcggcc gccggtagct gaatcgtgtt gaacggatcg
    16861 ccgttcttct cgacgcactg ctgcatgcgg gcagcgacga gcggatcggt gtgcggcgcg
    16921 aacgggagct tgtcgatcac gacgaggctg agcgcatcgc cgggaacgtc gacgccttcc
    16981 cagaacgagg cagtgcccag gagcacggcg ttcggcgtcg agcgaaagcg ctcgatcaac
    17041 gtcgtgcgcg gtgcgtcacc ctggacgaag cgcgcgtacg gagcatcctt caggcgggtc
    17101 gcggcatcgc gaagcgcgcg atgagacgtg aacaagagga atgcgcgccc ggatgtgagc
    17161 gccagcagct cgagcgtgcg ctgcaccgcc gctgcactga agccatcgtc ggcggggact
    17221 ggcagatcgc gtgggacgta cagcatcgcc tgcgtcgcgt agtcgaacgg cgacggcacg
    17281 agcagctcgt ccgcgtccgc gcccagcccg agccgcgcgc gctgatacga gaagtcaccc
    17341 gctgctgcga gcgtcgcgga tgtgaagatc ggcgtcgggc ccgacatcac gatgtggcgg
    17401 cgcacgagct cgccgacttc gatcggagat gcggtcagtg cggtccctgt cggccgcgtc
    17461 tcgccccagt acacgtgact gcgcgcgcgc tgttcggcga tcgttgcgag atcgtcgcgc
    17521 agctctcgcg cccgccttgc caatcccgtg agcgtcgcgc gtgcgcctgc gtcttcgttg
    17581 ggctcttcgt cgggcggcgg ctcgctctcg gcttcggccg tgcgcgcgag ctcctcgaga
    17641 gcggtgtcga gcttgaacca tgcggtctgt cgctcgggat gctcgaacaa cccttcgggc
    17701 aggagcacgc ggtcggcttc cccggcaatc gcgccgcgta tatgcgagaa cagcgcgagg
    17761 cccgcgcgtt cgaccgcggc gatcgtgtcg tcggccgcgc gcccggtcca cagcggcatg
    17821 cgcgacagcg agagatgcgc atcgcgaacg agctgcgaga gcttgtgcgt cgagacgcgc
    17881 gcgccgaagt gctcggtggc gacgtcctcg agctgatgcg cctcgtcgaa gatgaccgcg
    17941 tcgtgctcgg gcaatacgcg tgcaccggga tgtgccgctc gcaacgcgcg gtcggcgaag
    18001 tagaggtggt gattgacgag gatcagctcc gccttctcgg cgagccggcg cgcttgcgtg
    18061 acgaagcagc gctcgaagta cggacagcgc ggaccgatgc gtgcgtcagg cgttgtcgtg
    18121 acgtcggtcc agaccggcgc ggactcggcg agccactcga cctcggagcg atcgccggtc
    18181 tccgacagct cgctccactc gcggagcgcg acgagctctt cactggcacc gcgcgacagc
    18241 gcttcgccaa ggcgtcgacg acacacgtag tttgcgacgc ccttgagcac gaccgcgttg
    18301 aacggccggc cgaggatcat ccgcagcagc gggatgtcgt agcgcgcgat ctgatcctgc
    18361 agcgcgcggg ttcccgtcga cacgacgacg cgctttcctg actgcagcgc aggcacgaga
    18421 tacgcgagcg tcttgccggt gcccgtgccg gcctcgacga tgagaggacg ttggtcctcg
    18481 agcgcccgcg cgactgccgc cgacatcgtg cgctgctcga cgcgatcctc gtagtaggga
    18541 atcgcgcgct cgaggcctcc gcccggtgcg agcagctcgg tcatgccggt gtcttcggcg
    18601 gccgcgacgc cttttcggta tggccactcg tgccgaagcg ccgcgcgagc tcgtcgagaa
    18661 cccgctggat cggaatgcgc cgcgccgtga ggccgaccat gacgtggaag accaagtccg
    18721 cggtctcgta gatgactttg tcgtcggggc cgctcggcag ctc
//
LOCUS       DATLBZ010000074        11475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5278854, whole genome shotgun sequence.
ACCESSION   DATLBZ010000074 DATLBZ010000000
VERSION     DATLBZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11475
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5278854"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..898
                     /locus_tag="VMZ53_04010"
     CDS             209..898
                     /locus_tag="VMZ53_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HUS27641.1"
                     /translation="MSEAREQITTARKLVAEFLGTALLVAAVVGSGVMAQMLTRTPAI
                     QLLCNSIATGGALVAILLVFGPVSGAHLNPAVTVSMAATRDISWRLAAGYILVQAAGA
                     VGGAALANAMFDHAPLELATNVREGFPLLLGEAVATFGLVGVVIAVGRTRPSIAPLAV
                     AAYITAAYWFTSSTSFANPAVTLGRAFSDTFAGIRPSDVPAFIVAQAVGAACGALLFT
                     WLVPLRRPATA"
     gene            complement(895..2964)
                     /locus_tag="VMZ53_04015"
     CDS             complement(895..2964)
                     /locus_tag="VMZ53_04015"
                     /inference="COORDINATES: protein motif:HMM:NF044447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase"
                     /protein_id="HUS27642.1"
                     /translation="MRWIALAVLAAACAQQGATPSGDDAPPTVPDSVPGLGAHWTPDG
                     TAVDFRVASSRATRIELSIFGEADGEPLSRIDLARGDGDVWTTRIASSELPPVIYYGY
                     RVWGPNGTAPVDSDGNRMNPDKLLFDPYARELSHDPASGAPAKGIVLPELEPITDRPT
                     RALADAIIYEVHLRGFTKGADMPCAGTYAGAAEKADYLAALGVTAVEFLPIQEASNDR
                     NDTDPSSSSGDNYWGYSTLAYFAPDRRYACDQTPGGPTRELRDMVHAFHARGIEVYVD
                     VVYNHTAEGSGGILSLRGFDNAGYYQLDRAGTGFTNSNGVGADLATFDKPLARDLLFD
                     SLRYWRDTLGVDGFRFDLAPVLGNACGPGCFKFDPALHERLAIEFADIDLIAEPWGVV
                     AGSYQLGNHPKGWSEWNDKFRDAIRQDQNKTPVTPGWLADRITGSSDLYRPDGRAPSA
                     SINYLVSHDGFTLHDLYACNAPNNGQAWPYGPSDGGSQSNLSWDHGADATAQRQAVRT
                     GLALLLVSSGVPMITGGDELGRSLRCNNNPYNLDSPATWLDWSKQDTALWTFASRLFH
                     FRQAHRALHPATWIEPSQISWRDASGAVLTGAAMDDASKPVLAWRLDGASLGDDTLYV
                     AYNHSAQQQRITLPAPPAGLAWYRVADTAAWMEAEANFSAPGQEHKMQQASYDLVARS
                     LAIFVAR"
     gene            complement(3066..3953)
                     /locus_tag="VMZ53_04020"
     CDS             complement(3066..3953)
                     /locus_tag="VMZ53_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27643.1"
                     /translation="MRLSCMLVALVGCGRLAFDPLSTGGDGGDGQSGDAQGACAFGPW
                     STPIALPINSASDEFGATPSPDGLELTFHSNRIGGNHDLYVATRATHDDDFGNVQPLT
                     VVNSTSVDENATFAPDGLTLFFTSDRAGGSRLYKSTRASVGAAWSAPVMVPELASVAL
                     SGPVLSKQGTELYVTIGSSGVFHVERATYDGSTFTQPAIIPELGTTLQRGYPGISPDG
                     LTLAYEGQRTSYEISFVTRPTIDAPWGAESPFVEVNDASANEDVEWSYDGTELFFTSN
                     RMNVAGNLFDLFVVKRSCL"
     gene            complement(3966..6224)
                     /locus_tag="VMZ53_04025"
     CDS             complement(3966..6224)
                     /locus_tag="VMZ53_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS27644.1"
                     /translation="MEARALIKKHGVPRVVYAAALLGALLAALLGAWFVSGWHDVRLR
                     QDQARSAPLKAAETKADELGRELRSELEALIAREVKRPYFHYQNLMHDPRASIGVSVS
                     PSPLANGPEDRLVAGYFQIDSTGRTTTPTINDDVPELSEPENLAGNRAFRDQVTRDLV
                     AVLTPPRLPTTSAVVAIADPKPKHTRPIPPIATQSTDTGSTPSGPGSATTTNATNATN
                     ATNPTTAIQAQQAQVYEIDPNSYIQNNYSNSVYWQQHDNNNNAGVFVPPGNNAHRDND
                     PHRENTSPQIGNTPPRGSDKPTPENIDSPRGSDKPTPVNIDSPRGTDKPTPENVDSSG
                     GAKPDASPSVDSPVAAPAAAVETPRPKPAVRAAIKSVPPPQPQSVTITVSALAWQTMP
                     FGGSPGLIAVRQVQTPDGNLTQGFVIDRSTLTSQLAGKAGDMVAELRLDHRDAAMSAN
                     GASDETSSSMAPASLASRSVEIAPGWQLTVAPNPRTILQAARDANDIARAFLLRFILV
                     GAIGLIAACAVLWLVMRAEALARERSQFAAAAAHELRTPLAGLQLYGDMLADGLGDQA
                     KVRDYARRMSEEASRLGRVVSNVLGFSQLERGNLSVDAQVGPLGEALCELADRALPAL
                     DRAGAVLDLDVAPDVRARFDRDALARIVGNLLDNAEKYSREAEDRTIRLAVVDKGDVI
                     DVIVSDKGPGVAKGATLFKAFSRGVPADGPAGLGLGLALSRSLAKAMGGELLHADVDG
                     GGAAFTLRLPRA"
     gene            complement(6206..6886)
                     /locus_tag="VMZ53_04030"
     CDS             complement(6206..6886)
                     /locus_tag="VMZ53_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS27645.1"
                     /translation="MRILVVEDEASLREGIVDLLEGDGHQVTAVGDGAAGAEIGMRDP
                     FELVLLDLMLPKLDGMEVCRRLRAARPGMPILMLTARGSEDDKVRGLVEGADDYVTKP
                     FSARELLARIRVLGRRVQPGESLDEVQIDGAVIDLARMTVVRGDKRVALTPREVGIVR
                     WLHRHQDRVVTRAELLEQVFGQRGDLQTRAVDMAIAVLRKKLEADPQNPTLIVSVKGA
                     GYAWKLAR"
     gene            6951..8192
                     /locus_tag="VMZ53_04035"
     CDS             6951..8192
                     /locus_tag="VMZ53_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS27646.1"
                     /translation="MRRGLLALVLAGVVAGPTVASAEPTQIGGFFGPKIFSGDSALGY
                     LPDAPAHPTLHNSVQFGGRFGKPFGYIWLVPELEVSFVPTETTDAGGATPASVYWFTG
                     RVHLRLDLLPKRKLNAFLVVGGGADISASTARKTFNSGVIGEGYVGAGVLFDTQKGFL
                     VRFDTRFAGIPSATKTIAFEGDFMVGIEFLIGAPKKETKSEVVVQGPPPDKDDDGIAD
                     ADDKCIDRAEDSDNVEDGDGCPDIDNDGDHVLDIADKCAGEQETFNGFNDDDGCADSV
                     PAEVDALRGTVEGLIYAEGETVVRDSAQKNMAAIAKVMAANPSLKIVLVGHTDDREAK
                     AFAETPKEGEPPPDIDSIATDLARARAEAVRQALVAQGVPAGRVVVDGVGAEEPVADN
                     NSAKGRLANRRVELKLFVPKY"
     gene            complement(8196..8618)
                     /locus_tag="VMZ53_04040"
     CDS             complement(8196..8618)
                     /locus_tag="VMZ53_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27647.1"
                     /translation="MPLVAIAEVTPAAIITTGLSLLLTALMFLNPLSIAGQVVIGLLL
                     GVFGWALYMSLEKANAVRDAPVERVVAVIVKERVDLRRRLGDGRDTQRTYALVQTRDG
                     ERREYFVSKHAERRYAVDDIGIAYVKADVLIDFLRIDV"
     gene            complement(8706..9812)
                     /gene="dnaJ"
                     /locus_tag="VMZ53_04045"
     CDS             complement(8706..9812)
                     /gene="dnaJ"
                     /locus_tag="VMZ53_04045"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HUS27648.1"
                     /translation="MRDLYEVLGVERGASQADLKKAYRRLAQQFHPDKCGGDKEAEEK
                     FKEAANAYQILSDEEQRAAYDRYGFDGIRRGGGGPGPGFSNVEDIFSAFGDLFGDFFG
                     GRSSGKRQPRGADLRVDLELSFAEAVWGCSKEVKVTRAIACTTCSGSGAAAGSKADIC
                     RTCQGKGQVVHSQGFFMVQTTCPHCRGAGKTITNPCEDCRGRGLKPETSELNVTVPAG
                     VDDGQTLRLAGKGESVANGVTGHLYVVLHVQGDERFKRDGDDILTEIPISFVKAALGG
                     EVEVYTLEDNCNGVATVELKPGTQPGDVIVRRGQGIQHVGEHGRGDQVIQFKVEIPKK
                     LSSRQEELLRELATELNEDGVRPEKRGLFGRSKK"
     gene            10015..>11475
                     /locus_tag="VMZ53_04050"
     CDS             10015..>11475
                     /locus_tag="VMZ53_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27649.1"
                     /translation="MLACQRAPYLATLLTRDPLRLGRVAEDPYLRREKPLDHLMGQVR
                     AALSGVTTGAELRTALRRIRADELVRLGVRELELGLDTEVGRELSRLADVCFDAAIEF
                     HDRELRARYGAPRYHDDDGTEKDAKLAVIGMGKLGGEELNFASDVDVIYVYSSDQGEA
                     GSMSLHEYFAKLCTLVTSALSEVTEDDVVFRVDLRLRPEGAKGAIANSLAQAERYYET
                     FGRPWERQAWIKARACAGDHALGHETLEMLKPFVFPRYTSHAIVDEVKALNRRIKREL
                     VRTREGFDLKNGEGGIREIEFFTQALQLIHGGKRPVLRCRSTLAALDALLFAGLVSDD
                     EHLSLWRAYRWLRHAEHLLQLEGGLQTQTIPADPALQDIFARRLGYVDRDALQRMLVA
                     HTGSVARMFATLGVEVDLDRPDIDAILRGELSDEAEAATLARLGFRDVTAATAELARA
                     RRRPGSPLSPAASERAARIGSSLLSEIAASADPDQAL"
BASE COUNT         1825 a         3822 c         3878 g         1950 t
ORIGIN      
        1 gcgccgcgac ggcacgtggg tgtactaccg cctcgccgag caagagcacg cagccgtcca
       61 gacgacgctg gccgcgttga ccgaagcgtt tggtgccgag cgcgcgattc gcgcggacca
      121 cgcaaagctc aagaagagct gtggcccgga tgcgtgcaaa tgaaccgatg agatgtcgcg
      181 cgccaaatcc gatgagcgag gcgttgccat gagcgaggcg cgcgagcaga tcacgaccgc
      241 gcggaagctc gtcgccgagt ttctcggcac ggcgttgctc gttgcggcgg tcgtcgggtc
      301 gggcgtgatg gcgcagatgc tgacgcgcac gcccgcgatc cagttgctct gcaattcgat
      361 cgcaacgggc ggcgcgctcg tcgcaatcct cctcgtcttc ggccccgtct cgggcgcgca
      421 cctcaatccc gcggtcacgg tcagcatggc agcgacgcgc gacatctcgt ggcggctcgc
      481 cgctggctac attctcgtgc aagcggcagg tgccgtcggc ggcgccgcgc tcgcgaacgc
      541 gatgttcgat cacgctccgc tggagctcgc gaccaacgtg cgcgaaggct tcccgctgct
      601 tctcggtgag gccgtcgcga cgttcgggct tgtcggcgtc gtgatcgcgg tcgggcgcac
      661 gcgtccgtcg atcgcgccgc tggcggtcgc cgcgtacatc acggcagcgt actggttcac
      721 ctcgtcgacc tcgttcgcga atcccgccgt cacgctcggc cgcgcattca gcgacacgtt
      781 cgcaggaatt cgcccctccg atgttccagc gttcatcgtc gcgcaagccg taggcgcagc
      841 gtgtggcgcg ctgctgttca cgtggctcgt gccgcttcgt cggcctgcaa cggcttagcg
      901 cgcgacgaag atcgcgagcg accgggcgac gaggtcgtag ctcgcctgct gcatcttgtg
      961 ctcttggccc ggcgcgctga agttcgcctc ggcttccatc cacgcggccg tgtcggcgac
     1021 gcgataccac gcgagccccg cgggtggcgc cggcagcgtg atgcgttgct gctgcgcgct
     1081 gtggttgtac gcgacgtaca gcgtgtcatc gccgagagac gcaccgtcga gtcgccacgc
     1141 gagcacaggc ttggacgcgt cgtccatcgc ggcgccggtg agcacagcgc cggacgcgtc
     1201 gcgccacgag atttgcgacg gctcgatcca cgtcgcgggg tgcagagcgc ggtgcgcttg
     1261 gcggaagtgg aacaggcgcg acgcaaacgt ccagagcgcg gtgtcttgct tgctccagtc
     1321 gagccacgtc gcgggcgagt cgaggttgta tggattgttg ttgcagcgaa gcgaacggcc
     1381 gagctcgtcg ccgccggtga tcatcggcac gccggacgag acgagcagca gcgcgagccc
     1441 cgtgcgcacc gcttggcgtt gtgcggttgc gtcggcgccg tgatcccagg agaggttgga
     1501 ttgcgagccg ccatcgctcg gaccgtaggg ccacgcttgg ccgttgttcg gtgcgttaca
     1561 cgcgtagaga tcgtggagcg tgaagccgtc atgcgagacg aggtaattga tcgacgcgga
     1621 cggtgcgcgg ccgtcggggc gatacagatc gctcgagccg gtgatgcggt ccgcgagcca
     1681 gccgggcgtg acgggcgtct tgttctggtc ttggcggatc gcgtcgcgga acttgtcatt
     1741 ccactcgctc cagcctttgg ggtgattgcc gagctggtag ctccccgcga cgacgcccca
     1801 cggctcggcg atgagatcga tatcggcgaa ttcgatcgcg agccgctcgt gcagtgcggg
     1861 atcgaacttg aagcagccgg gcccgcacgc attgccgagc accggcgcga ggtcgaagcg
     1921 aaacccgtcg acgccgagcg tgtcgcgcca gtagcgcagc gaatcgaaga gcagatcgcg
     1981 cgcgagcggc ttgtcgaacg tcgcgagatc ggcccccacg ccgttgctgt tcgtgaagcc
     2041 ggtgcctgcg cgatcgagct ggtagtagcc cgcgttgtca aagccgcgga gcgagaggat
     2101 gccgccgctg ccttccgcgg tgtggttgta aacgacgtcg acgtagacct cgatgcctct
     2161 cgcgtggaac gcgtgcacca tgtcgcgcag ctcgcgcgtc ggcccgccgg gcgtttgatc
     2221 gcacgcgtag cggcgatcgg gcgcgaagta cgcgagcgtc gagtagcccc agtagttgtc
     2281 gcccgacgac gagctcggat cggtgtcgtt gcgatcgttg gacgcttctt gaatcggcag
     2341 aaattcgacg gcggtcacgc cgagcgcggc gaggtagtcc gctttctccg ccgcgcctgc
     2401 gtaggtgcca gcgcatggca tatcggcgcc cttcgtaaag ccgcgcaggt gcacctcata
     2461 gatgatcgcg tccgccagcg cgcgcgttgg ccgatcggtg atcggctcca gctccggtag
     2521 cacgatgccc ttcgccggcg cgcccgacgc tggatcgtgt gacagctcgc gcgcgtatgg
     2581 atcgaacagc agcttgtcgg gattcatgcg gttgccgtcg ctgtcgacgg gcgccgtgcc
     2641 gttcggtccc cacacgcgat agccgtaata gatgacgggc ggcagctcgc tcgaagcgat
     2701 gcgcgtcgtc cacacatcgc cgtcgccgcg cgcgaggtcg atgcgcgaga gcggctcgcc
     2761 gtctgcctcg ccgaaaatcg acagctcgat gcgcgtcgct cgcgacgacg ccacgcgaaa
     2821 atcgaccgcg gtgccatccg gcgtccaatg cgcgccgagc cccggtacgc tgtcgggcac
     2881 cgtcggcggc gcgtcgtctc ccgacggcgt tgcgccttgc tgcgcgcacg cagcagcgag
     2941 gacagccaag gcaatccacc gcatcacgcg cgagtgctgc aacgcgcgcg ccgctgcagt
     3001 ctcgcgattg cgcgttgccc gcgggccgcg atgttgccga acgcaacgcg cgctggcccg
     3061 gattgctaca ggcagctgcg cttcacgacg aacaggtcga aaaggttgcc ggcgacattc
     3121 atccggtttg acgtgaagaa gagctcggta ccgtcatacg accactcgac gtcctcgttg
     3181 gcactcgcgt cgttcacctc gacaaacggg gactcggcgc cccacggcgc gtcgatcgtc
     3241 ggacgcgtca cgaagctgat ctcgtacgac gtgcgttggc cctcgtacgc gagcgtcagc
     3301 ccatcgggcg agataccggg atagccacgc tgcaacgtcg tgccgagctc gggaatgatc
     3361 gctggctgcg tgaatgtcga gccgtcgtac gtcgcacgtt cgacatggaa caccccagac
     3421 gaaccgatcg tgacgtatag ctcggtgccc tgcttcgaca gcactggacc actcagcgcg
     3481 acgctcgcaa gctcgggcac catcaccggt gcgctccacg ccgcgccgac tgacgcgcgc
     3541 gtcgacttgt acagtcgcga cccacccgcg cggtcgctcg tgaaaaacag cgtcagcccg
     3601 tcgggcgcaa acgtcgcgtt ctcatcgacc gacgtcgaat tcaccacggt gagcggttgg
     3661 acattgccga agtcatcgtc atgcgtcgcg cgagtcgcga cgtacaggtc gtggttgccg
     3721 ccgatgcggt tgctatgaaa tgtgagctcg agaccgtcgg ggctcggcgt cgcgccgaac
     3781 tcgtcggatg cggaattgat cggcaacgcg atcggcgtgc tccatggtcc gaacgcgcat
     3841 gccccttgcg cgtcgccgct ctgtccgtcg ccgccgtcgc cgcccgtgct cagcggatcg
     3901 aacgcaagcc gcccgcatcc tacgagcgcg acgagcatgc acgacaagcg cacgccgcca
     3961 ttgtctcacg ctcttggcag acggagcgtg aacgccgccc cgccaccatc cacgtcggcg
     4021 tgcagcagct cgccgcccat cgccttcgcg agcgagcgcg acagcgcgag cccgaggccg
     4081 aggcccgcgg gaccatccgc cggcacgccg cgcgagaacg ccttgaacag cgtcgcgccc
     4141 ttcgccacgc ctgggccctt gtcgctgacg atcacgtcga tgacgtcgcc tttgtcgacg
     4201 acagcgagcc gaatggttcg gtcttccgcc tcgcgactgt acttctcggc gttgtcgagc
     4261 aagttgccga cgatgcgcgc gagcgcgtcg cgatcaaagc gggcacgcac gtccggcgcg
     4321 acgtcgaggt ccaacaccgc gcccgcgcga tccaatgcag gcaacgcgcg atcggcgagc
     4381 tcgcacaacg cctcaccaag cggccccacc tgcgcatcga cgctcaagtt gccacgctcg
     4441 agctgcgaga acccgagcac gttcgagacg acgcgcccca accgcgacgc ctcttcgctc
     4501 atgcggcgtg cgtagtcgcg aaccttcgcc tggtcgccga gcccatcggc cagcatgtcg
     4561 ccgtagagct gcagccccgc gagcggcgtg cgcagctcgt gagccgcagc cgctgcaaac
     4621 tggctgcgct ctcgcgcgag cgcctcggcg cgcatgacga gccacagcac cgcgcacgcg
     4681 gcgatgagcc cgatcgcacc gacgagaatg aagcgaagca gaaacgccct cgcgatgtcg
     4741 ttcgcgtcgc gtgccgcctg caagatcgtc cgcggattcg gcgcgacggt gagctgccaa
     4801 cccggcgcga tctcgacgct gcgactcgcg agcgaagccg gcgccatcga cgagctcgtc
     4861 tcatcgctcg cgccgttcgc gctcatcgcc gcatcacgat gatcgaggcg gagctccgcg
     4921 accatatcgc ctgctttgcc ggcgagctgg ctggtcagcg tcgagcggtc gatcacgaaa
     4981 ccttgcgtca ggttgccgtc gggcgtctgc acctggcgca ctgcgatgag cccgggcgag
     5041 ccgccgaatg gcatcgtctg ccacgccagc gccgacacgg tgatcgtcac ggactgcggc
     5101 tgtggcggtg gtacggactt gatggccgcg cgcacggccg gcttcggcct cggcgtctcg
     5161 acagccgcag ctggagcagc aaccggcgag tcgacagaag gcgacgcatc cggtttcgcg
     5221 ccacccgacg aatcgacgtt ctctggcgtc ggcttgtcag taccacgcgg tgaatcgatg
     5281 ttcaccggcg tcggcttgtc gctaccgcgc ggtgaatcga tgttctccgg cgttggcttg
     5341 tcgcttccgc gcggcggcgt gttcccgatc tgcgggctgg tgttctcgcg atgagggtcg
     5401 ttatcgcgat gggcgttgtt gcccggcggc acgaagacgc ccgcgttgtt gttgttgtcg
     5461 tgctgctgcc agtagaccga gttcgagtag ttgttctgga tgtagctgtt cggatcgatc
     5521 tcgtagacct gcgcctgttg cgcttggatc gcggtggtcg ggttcgttgc gttggtcgcg
     5581 ttcgtcgcgt tcgtggtcgt cgcggagccg gggccggacg gcgtcgagcc cgtgtccgtc
     5641 gactgggttg cgatcggcgg gatcgggcgc gtgtgcttcg gctttggatc ggcgatcgcg
     5701 acgacggcgc ttgtcgtcgg caggcgcggc ggcgtgagca ccgcgacgag gtcgcgcgtc
     5761 acttgatcgc ggaatgcgcg attgccggcg aggttttccg gctcggagag ctcggggacg
     5821 tcgtcattga tcgttggcgt cgtcgtgcgg cccgtcgagt cgatctggaa gtagcccgcg
     5881 acgagccgat cctcgggacc attcgcgagc ggcgatggcg agacactgac gccgatggac
     5941 gctcgcggat cgtgcatcag gttctggtag tggaagtacg gccgcttcac ctcgcgcgcg
     6001 atcagcgctt cgagctcgct gcgcagctcg cggcctagct cgtccgcttt cgtctcggcc
     6061 gctttgagcg gcgccgaacg cgcttggtct tgccgcaggc ggacgtcgtg ccagcccgag
     6121 acgaaccacg cgccgagcag cgcggcgagc agtgcgccga ggagtgcggc ggcgtagacg
     6181 acgcgcggaa cgccgtgctt cttgatcagc gcgcgagctt ccatgcgtag cctgcgcctt
     6241 tgacggacac gatcagcgtc gggttctgcg gatcggcttc cagcttcttg cgcagcaccg
     6301 cgatcgccat gtcgacggcg cgcgtttgca ggtcgccgcg ttggccgaag acttgttcga
     6361 gcagctccgc gcgcgtgacg acgcgatctt gatggcggtg cagccagcgc acgatgccga
     6421 cctcgcgagg cgtcagtgcg acgcgcttgt cgccgcggac gaccgtcatg cgcgcgagat
     6481 cgatcaccgc gccgtcgatc tggacctcgt cgagtgactc gccgggctgg acgcggcggc
     6541 cgagcacgcg aatgcgcgcg agcagctcgc gggcggagaa cggcttggtc acgtagtcgt
     6601 cggcgccctc gaccaggccg cggactttgt cgtcttccga gccgcgggcg gtgagcatca
     6661 ggatcggcat gcccgggcga gcggcgcgca ggcggcggca gacctccatg ccgtcgagct
     6721 tggggagcat caggtcgagg aggacgagct cgaagggatc gcgcatgccg atttcggcgc
     6781 ctgcggcacc gtcgccgacc gccgtgacct ggtggccgtc gccctcgagc agatcgacga
     6841 tgccctcgcg caggctggct tcgtcttcga ccacgagaat gcgcacgaag tcattgtgtc
     6901 acgcggccca ggcgcgcgtg taaggcgcgt gtaaggctat actccgcacg atgcgtaggg
     6961 ggctcctcgc gctcgtgctt gctggcgttg ttgccggccc aacagtcgcg tccgcagagc
     7021 cgacgcaaat cggcggattc ttcggaccga agatcttcag cggcgattcc gcgctcggct
     7081 atctgccgga cgcgcccgcc cacccgacgc tccataactc ggtgcagttc ggcggccgct
     7141 tcggcaagcc gtttggctac atctggctcg ttccggagct cgaagtctcg ttcgttccga
     7201 cggagaccac ggacgccggt ggcgcgacgc ccgcgagcgt ctactggttc accggccgtg
     7261 tgcacttgcg cctcgacctc ttgccgaagc gcaagctcaa cgcattcctc gtcgtcggtg
     7321 gcggcgcaga catctctgcc tcgaccgcgc gcaagacatt caactccggc gtgatcggcg
     7381 aaggttacgt cggtgcgggc gtgctgttcg atacgcagaa gggcttcctc gttcgcttcg
     7441 atacgcggtt cgccggcatt ccgtcggcca cgaagacgat cgcattcgag ggcgacttca
     7501 tggtcggcat cgagttcctg atcggcgcgc cgaagaaaga gacgaagtcc gaagtcgtcg
     7561 tccaagggcc gccgcccgac aaagacgacg acggcatcgc cgacgccgat gacaagtgca
     7621 tcgatcgcgc ggaagactcc gacaacgtcg aggacggcga cggctgcccc gacatcgaca
     7681 acgacggcga tcacgttctc gacatcgcgg acaaatgtgc cggcgagcag gagacgttca
     7741 acggcttcaa cgacgacgac ggctgcgccg acagcgtgcc cgccgaggtc gacgcactcc
     7801 gcggcaccgt cgaaggtctc atctatgccg agggcgagac ggtcgtgcgc gactccgcgc
     7861 agaagaacat ggcggcgatc gcgaaggtga tggcggcgaa tccgtcgctg aagatcgtgc
     7921 tcgtcggtca caccgacgat cgcgaggcaa aagcatttgc ggagacgccc aaagaaggcg
     7981 agccgccgcc cgacatcgat tcgatcgcga cagacctcgc acgcgctcgt gcggaagcgg
     8041 ttcggcaagc gctcgtcgcg caaggcgtgc ccgcaggccg cgtggtcgtc gacggtgtcg
     8101 gcgccgaaga gcccgtcgct gacaacaact cggcgaaggg ccgcctcgca aaccgccgcg
     8161 tcgagctgaa gctgttcgtc ccgaagtatt gagcgtcaga cgtcgatgcg caggaagtcg
     8221 atgagcacgt cagctttgac atacgcgatg ccgatgtcat cgacggcgta tcgccgctcg
     8281 gcgtgcttcg acacgaagta ctcgcgccgt tcgccatcgc gcgtctgaac gagcgcgtac
     8341 gtgcgctggg tatcgcgtcc atcgccgagg cgacgtcgaa gatcgacgcg ctccttgacg
     8401 atgactgcga ccacgcgctc gaccggcgcg tcgcggacgg cgttcgcttt ctcgaggctc
     8461 atgtagagcg cccagccgaa cacgccgagc aaaagaccga tgactacctg acccgcgatc
     8521 gagagcgggt tcaagaacat cagcgcggtg agcaggagcg agagacccgt cgtgatgatc
     8581 gcggccggcg tgacctctgc gattgcaacg agcggcacac cgactggtac ctccggcgca
     8641 gacgagtacc tcgggtgctc gaggacacga tcgaaacgct ccggttcggt gcccatagcg
     8701 gagagctact tcttgctgcg accgaagagg ccgcgcttct cgggccgcac gccatcttcg
     8761 ttcagctcgg tcgcgagctc gcgcagcagc tcttcctgcc gcgacgacag cttcttcgga
     8821 atctcgacct tgaactggat cacctgatcg ccgcggccgt gctcgccgac gtgctggatg
     8881 ccctgcccgc ggcgcacgat cacgtcgccg ggttgcgtgc cgggcttgag ctcgacggtc
     8941 gcgacaccgt tgcagttgtc ctcgagcgtg tagacctcga cctcaccgcc gagcgccgct
     9001 ttgacgaagc tgatcggaat ctcggtgagg atgtcgtcgc catcgcgctt gaaccgctcg
     9061 tcaccttgga cgtgaagcac gacgtagagg tggcccgtca cgccatttgc gacggactcg
     9121 cctttgcccg cgaggcggag cgtctgaccg tcgtcaacgc cggcgggcac cgtcacgttg
     9181 agctcggagg tctccggctt gagaccgcga ccgcggcagt cctcgcacgg attcgtgatc
     9241 gtcttgcccg cgccgcggca gtgcggacac gtggtctgca ccatgaagaa gccctgcgag
     9301 tgcacgacct ggcccttgcc ctgacacgtg cggcagatgt ccgccttgct gccagcggcg
     9361 gcgccggacc cgctgcatgt cgtgcacgcg atcgcgcgcg tgaccttgac ctctttgctg
     9421 cagccccaca cggcctcggc gaacgacagc tcgagatcga cgcgaagatc cgcgccgcgc
     9481 ggctgacgct tgccgctcga gcgaccgccg aagaaatcac cgaacagatc gccgaatgcc
     9541 gagaagatgt cttcgacatt cgagaagccg ggaccgggac cgccaccgcc gcggcggatg
     9601 ccatcgaagc cgtagcgatc gtaggccgcg cgttgctctt cgtcggacag gatctggtac
     9661 gcgttcgccg cttccttgaa tttttcctcg gcctctttgt caccgccgca cttgtcggga
     9721 tgaaattgct gcgcgagccg gcggtaggct ttcttcaagt cggcttggct tgcgccacgc
     9781 tcgactccga gtacttcata gagatcacgc atgaccgagg agaatcaacg ccttgggtaa
     9841 tgcgggaccg cgtgggtgtc aaggtatggt gaccgcatgg tcaggcctgc tgatctcgcg
     9901 atcggcgccc cggatgaggg ggatgcgctc cggcgcgccg accggttgtt cgacgccgcg
     9961 tcccaaagcg gcctggatct caccgacttg gacgagaccg ctgcccaggt cgcgatgttg
    10021 gcctgccagc gtgcaccgta tctcgccacg ctcctgaccc gagaccctct ccgattgggg
    10081 cgggtggcag aggacccgta cctgaggcgc gagaaaccac ttgaccacct gatgggccag
    10141 gtccgtgctg ccctctcggg cgtcacgacc ggcgcggagc tgcgcaccgc tctcaggcga
    10201 attcgcgcag acgagctcgt ccggctgggc gtgcgcgagc tcgagctcgg cctcgatacg
    10261 gaggtcggcc gcgagctgtc gcgcctggcg gacgtctgct tcgatgccgc gatcgagttt
    10321 cacgatcgcg agctccgcgc gagatacgga gcgccgcggt accacgacga cgacggcacg
    10381 gagaaagacg cgaagctcgc cgtgatcggc atgggcaagc tcggcggcga ggagctcaac
    10441 ttcgcgtccg acgtcgacgt catctacgtc tactcgagtg atcagggcga agccggcagc
    10501 atgtcgctgc acgagtactt cgcgaagctg tgcacgctcg tgacgagtgc gctctccgag
    10561 gtcaccgagg acgacgtcgt gtttcgcgtc gacctgcgcc tgcgccccga gggcgcgaag
    10621 ggcgcgatcg cgaactcgct cgcgcaggcg gaacgctact acgagacgtt tggccggcca
    10681 tgggagcgtc aggcgtggat caaggcgcgc gcgtgcgcag gcgatcacgc cctcggccac
    10741 gagacgctcg agatgctcaa gccgttcgtg ttcccgcgct acacgtcgca cgcgatcgtc
    10801 gacgaggtga aggcgctgaa tcgccgcatc aagcgcgagc tcgtgcggac gcgcgaaggg
    10861 ttcgatctca agaatggcga gggcggcatt cgcgagatcg agttcttcac gcaggcgctg
    10921 cagctcatcc acggcggtaa gcgccccgtg cttcgctgtc gcagcacgct cgccgcactc
    10981 gacgcgctgc tgttcgcggg cctcgtctcc gatgacgagc atctctcgct gtggcgcgcg
    11041 tatcgctggc tgcgccacgc cgagcacttg ctgcagctcg aaggtggtct gcagacgcag
    11101 acgattcccg ccgatcccgc gctgcaagac atcttcgcgc ggcggctggg ctacgtcgat
    11161 cgcgacgcac tccagcgcat gctcgtcgcc cacactggct cggttgcacg catgttcgcg
    11221 acgcttggcg tcgaggtcga cttggatcga ccggacatcg atgcgatctt gcgcggcgag
    11281 ctgtccgacg aggccgaagc cgccaccctc gctcgcctcg gctttcgcga tgtcaccgcg
    11341 gcgacggccg agctcgcgcg agcgcgcagg cgtccggggt caccgctgtc gccggcggca
    11401 agcgaacgcg ccgcacgcat cggcagctct ctgctgtcgg agatcgcggc gagcgccgat
    11461 ccggatcaag cgctg
//
LOCUS       DATLBZ010000075         9982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5279750, whole genome shotgun sequence.
ACCESSION   DATLBZ010000075 DATLBZ010000000
VERSION     DATLBZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5279750"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="VMZ53_04055"
     CDS             <1..1900
                     /locus_tag="VMZ53_04055"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HUS27650.1"
                     /translation="MRVTVFFMFVVGLAACGPNGRNNGSNCAGLCTALGYQACHEDGR
                     YEPPVSCGPDQRCDPNAGCVVCPPEGLYCGGATDNDVFRCNKQGTEGTLVESCAANEV
                     CSDGECKTPCEAAMDNPSNVGCDFWAVDLDNEISSIAGITTDSAANQFALVVANNNPY
                     PVTVTITKNGANIGQPLNEQAVASAVVSPGVAKRIDLPQREVDGCMAQNGPYMPRTGS
                     GTFVSPHAYHVTTTGPVVVYQFNPIIQAYSNDASTLIPIQALGMDYVVLGYQTANPCG
                     ISGFNLPDIPDHTAVTIVPIEDNTTVTVTATHPIVASAGPSGIAIPATPKGTPLTLKL
                     SRYMVANLESDQPKNVSATQCGSMVNGGGQNGDFTGTQIHSDKPIIVFTSGERGEGFG
                     GAQNIEYPPDFDSMSGDDTCCTDHLEEQLFPVTALGKEFAIARSPIRSTDQSGWVEPD
                     IMRVVATVDNTHITTNLPAPYNSITLNRNQQKTFGATTGFALKSDQPIEVGSFLVSQH
                     FIKHGYIGDPSQITLPAAEQFRKDYVFLVPATFTDNYMVVAKPVDAAVVLDGTPLMGA
                     EFSNCYAGSIGMVNGVMYDQLTCKLAEGQHTVSADKPFGLSVYGYYNVGSYAFVGGSD
                     VKIINPIL"
     gene            complement(1884..3377)
                     /locus_tag="VMZ53_04060"
     CDS             complement(1884..3377)
                     /locus_tag="VMZ53_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS27651.1"
                     /translation="MARLRFRHRIGLLLALAAVALIAVTVVTLALGRSSERQLVGIET
                     RYVPLIELNGDLKRMASELSKAFEDAADAGEESKLAEAEQLRAAFRARIDAGRSVIAR
                     NGADPDALLAALDRFAEAARPLSVELIRGRPASELTTEIGVMVQTRRDFLALINAATT
                     PDRARMTDAFARARAVQRTALVVNIVVALATLAIMALLSWHLIRRTVRSLQAVSEGVE
                     RLARGDFATPIDVPPGDEIGDLAREANRTADRLREYREQAAREAQAAATANKELEAFS
                     YSVSHDLRAPLRGIAGFSLALVEDESDKLSPKGHDYLNRIRAGAQRMGELIDDLLRLS
                     RVSRADFKKERVDVSSLATLVVEDLRKSAPERSVAVDIQPNVSASADPRLLRIVLENL
                     IGNAWKFTSKTESAKIEVGARDDDSEGGKQRVYFVRDNGAGFDMKYAERLFAAFQRLH
                     SDKEFPGTGIGLATVQRIVIRHGGRIWVEAAPSAGATFQFTLPRGSD"
     gene            complement(3377..3808)
                     /locus_tag="VMZ53_04065"
     CDS             complement(3377..3808)
                     /locus_tag="VMZ53_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27652.1"
                     /translation="MRALRLAIVVMLVLGLGSASIAAEEEFKVIVHPDISVDAVSRDF
                     LRDAYLKKATEWSSGTTVHPIDLSTKFGVRSAFIEKVLRKTPTQLRRYWNQQIFSGKG
                     VPPPEASSTDAVISYVLSHKGAVGYLPASADSGKAKVIKVQ"
     gene            complement(3810..5117)
                     /locus_tag="VMZ53_04070"
     CDS             complement(3810..5117)
                     /locus_tag="VMZ53_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27653.1"
                     /translation="MNRLRGAHHIITLVVVGVLAEPTSEAQPQPEGEPVETQAASPAV
                     LVEPPPPVTEEVDGQALAIHGFVSEGAFISTANDYLGVSSRGSVELFEAGLNVSKDIA
                     DRLHAGIQLYARDVGTFRDLPPRLDWAYLDYHWKAWLGLRAGVIKMPFGLYNEYADVD
                     AARTAILMPQSVYPLRDRSALLAQTGFSIYGERSLGACAGSLEYQAWLGTLNIPENAL
                     ELTGARLDDIDTKYVTGAQLFWHTPLDGLRIGGTFLRTSIDFKLTLDPSTVSALIMAG
                     LVPADFTGKLVISQRPDQLWIASAEYIHDDWSFAAEYSRAYKRQTSTLRTLLPTFEDE
                     AERFYALVTRRLSPAVELGAYYSVLYADVDDRHGRDMMKYAKPFAAWQRDASATLRFD
                     VNDRWLWKLEGHVIDGTADLFASKNPDPERWWGLFLVKTTVTF"
     gene            complement(5179..5622)
                     /locus_tag="VMZ53_04075"
     CDS             complement(5179..5622)
                     /locus_tag="VMZ53_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS27654.1"
                     /translation="MPAAKKILLVEDNPDDVELTLRAFERTKIANEVIVATDGEEALD
                     YMFASGKFAGRDPDAAPTVVLLDLNLPKLTGLDVLKRMRADARTRRLPVVMLTSSTEE
                     RDIIASYDLGANSFVRKPVDFAEFLDAARQLGMYWLVLNEPPPTR"
     gene            complement(5656..6333)
                     /locus_tag="VMZ53_04080"
     CDS             complement(5656..6333)
                     /locus_tag="VMZ53_04080"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HUS27655.1"
                     /translation="MNIGMLLFPRVTQLDLTGPYEVLTRIPGAIVHVVAKTRDPVAVE
                     VGGLQLVPTTTFSEAPPIDLLFVPGGGGQVDATDDNETLDWVHATGEKARWVTSACTG
                     SLLLGAAGLLTGYRAATHWAFMDLLSLVGAIPTAKRIVVDRNRMTSGGVTAGIDIAFT
                     IASEVAGRDVAELIQLQLEYDPEPPFRAGHPNVASPDLVARVRERVAQRYGQRLEQLR
                     RIAKLER"
     gene            6477..7877
                     /locus_tag="VMZ53_04085"
     CDS             6477..7877
                     /locus_tag="VMZ53_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HUS27656.1"
                     /translation="MSSVPSAIADAYSPKRLGAEGHRLIDAIAEQLASWQRRDGVALP
                     WRAPAEARAHWRLQDGGADLVEDLRRIMQSSTALYHPRVMAHQVPPPLPGAALAELVS
                     ALLNNGMAVYEMGPASVPIELETVDWMCKRLGYGDGAGGVFTSGDSLGNLTALLAMRQ
                     AKAGFDVWKQGAHAGPPLAVIVSSDAHYSVARTLRIMGWGDGGAISAQVDERHRLTAA
                     SVEAALATARDRRVIGIIAAAGSTATGAFDPLPELADLAEREGLWLHVDAAHGGGAAL
                     SAAHRHRLAGIERADSIVWDAHKMMMMPALVTAVLFKRGSHAYEAFAQQASYLFQAAA
                     PEDTWWDLGTRTLECTKRMMAIEVWTALRVHGEKLFGDIIDRLCALTRAFAERIAATD
                     DFELALEPELNIVCYRHKTADNRALRQRVVEDGRFYIVGTQLPRGYCLRSTIMNPLIE
                     PSDFDELLAHLRTLCR"
     gene            7868..8170
                     /locus_tag="VMZ53_04090"
     CDS             7868..8170
                     /locus_tag="VMZ53_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HUS27657.1"
                     /translation="MPVVTIIGENGPATVEVPANTTLFDAGAKLAAGIDTSCVGKGTC
                     GLCRVKILAGAEHLNPFTDEERKHLGNVYHITKVRLSCRSHVSGDVTIEVVRRKKK"
     gene            complement(8172..8792)
                     /locus_tag="VMZ53_04095"
     CDS             complement(8172..8792)
                     /locus_tag="VMZ53_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27658.1"
                     /translation="MNKFALVLLSLVPAVALAQPKAGAPATPPAPKADPKAPAAQPAP
                     PPAPVAAPAEVKATVEAFKGNWKFDASMSATGMPGMDKPMAGKMSFNCKEVAGKTGVS
                     CESKMKTPMGPMDALFVVAFNPYDKKVHFIGITNMNEVHDHLCTWKTATELTCEPLKA
                     GMGPGGDEVTEDVTITWTSKKEVAFKSVSKMTKGGATMTFEGKGKR"
     gene            complement(8833..>9982)
                     /locus_tag="VMZ53_04100"
     CDS             complement(8833..>9982)
                     /locus_tag="VMZ53_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS27659.1"
                     /translation="VATETGESKTSTGLFRKVGNDGLSRLEEVDNPTAVAVPRAKRAT
                     GQVDALQDDPNNRPSSPDSAMHQLLDARYDRDRRTDEAAPPAPTAPVPKADPTRPLRK
                     EPQPEPPPSRRGLLLLVLLVILGGGGFAIWKLTQKPAATTVVTANDGGTEIPVGFFVI
                     DPDPDGASVTLTDENGKVYELGKTSHDPKKLIRNKVSANAKFKIHIELPGYNPYDTES
                     TVLPNETLRISPRLEKASAKLHVETNPSGAQVAMGGKLLGETPLNRDGLEPKSGDLVI
                     TKQGYETVKIKITLALGKPVDIKRDLKELDKMGTVMIEVTGSASYAEAFFKGKNLGRT
                     RTMQGPQAFHLPVGSQQIKLVHPTDKKKTKTITVNVTESPQTIKASFD"
BASE COUNT         1719 a         3250 c         3242 g         1771 t
ORIGIN      
        1 gatgagggtg accgtctttt tcatgttcgt cgtcggtcta gcggcgtgcg gcccgaacgg
       61 gcgcaacaac ggaagcaact gcgcggggct ctgcacggcg ctcggctacc aagcgtgtca
      121 cgaggacggc agatacgaac caccggtctc gtgcggtccc gatcagaggt gcgatccgaa
      181 tgcgggctgc gtcgtgtgcc cgcccgaagg tctctactgc ggtggcgcga ccgacaacga
      241 cgtcttccgc tgcaacaagc aaggcaccga aggcacgctc gtcgaatcat gcgcggcgaa
      301 cgaagtctgc tcggatggcg agtgcaagac gccatgcgaa gccgcgatgg acaatccatc
      361 gaacgtcggt tgtgatttct gggcggtcga tctcgacaac gaaatctcct cgatcgccgg
      421 catcacgacg gactccgcgg cgaaccaatt cgcgctcgtc gtcgcaaaca acaatccata
      481 tccggtcacc gtcacgatta cgaagaacgg cgcaaacatc ggccagcctc tcaacgagca
      541 agccgtcgct tcggccgtcg tgtcaccggg cgtcgccaag cgcatcgatc tgccgcagcg
      601 cgaagtcgat ggctgcatgg cgcaaaacgg cccgtacatg ccacgcacgg gctcgggcac
      661 gttcgtctcg ccgcacgcgt accacgtcac gacgaccggc cccgtcgtgg tctaccagtt
      721 caacccgatc atccaggcgt actccaacga cgcgtcgacg ctcatcccga tccaagcgct
      781 cggcatggac tacgtcgtgc tcggctacca gaccgcgaac ccatgcggca tcagcggctt
      841 caacctcccc gatatcccgg atcacaccgc ggtcacgatc gtcccgatcg aagacaacac
      901 gacggtcacg gtgacggcaa cgcatccgat cgtcgcatcg gccggtccct ccggcatcgc
      961 gatcccggca acgccgaagg gcacgccgct gacgctgaag ctctcgcgct acatggtcgc
     1021 gaacctcgag tcggaccaac ccaagaacgt gtcggcgacg cagtgcggct cgatggtcaa
     1081 cggcggtggc caaaacggcg acttcaccgg cacgcagatc cactccgaca aaccgatcat
     1141 cgtgttcacg tccggcgagc gcggcgaagg cttcggcggc gcgcagaaca tcgaataccc
     1201 gcccgacttc gactcgatga gcggcgacga tacgtgctgc accgaccacc tcgaagagca
     1261 gctgttcccc gtcaccgctc tcggcaaaga attcgcgatc gcgcgcagcc cgattcgctc
     1321 caccgatcag tccggctggg tcgagcccga catcatgcgc gtggtcgcta ccgtcgataa
     1381 cacgcacatc acgacgaacc tcccagcgcc ctacaactcg atcacgctca accgcaacca
     1441 gcaaaagacg ttcggcgcga cgacgggctt cgcgctcaag agcgatcaac cgatcgaagt
     1501 cggctcgttc ctcgtgtcac agcacttcat caagcacggc tacatcggcg acccctcgca
     1561 gatcacgctt cctgccgccg agcagttccg caaagactac gtgttcctcg ttcccgcgac
     1621 gttcacggac aactacatgg tcgtggctaa accagtcgac gcagccgtcg tcctcgacgg
     1681 cacgccgctg atgggcgccg agttctcgaa ctgctacgca ggatcgatcg gcatggtcaa
     1741 cggcgtgatg tacgaccagc tcacgtgcaa gctcgccgaa ggccagcata cggtctcggc
     1801 tgacaagccg ttcggcctct cggtgtacgg ctactacaac gtcggcagct acgcgttcgt
     1861 cggcggctcg gacgtgaaga tcatcaatcc gatcctctag gcagcgtgaa ctgaaacgtc
     1921 gcgccggcgc tgggtgctgc ctcgacccag attcggccgc cgtggcgaat gacaatgcgc
     1981 tgcacggtcg cgaggccgat gcccgtgccg ggaaactcct tgtcggagtg gaggcgctgg
     2041 aacgccgcga acaaccgctc ggcgtacttc atgtcgaagc cggcgccgtt gtcgcgcacg
     2101 aagtagacgc gttgcttgcc gccttcgctg tcgtcgtctc gcgcgccgac ctcgatcttc
     2161 gccgactcgg tcttcgacgt gaacttccac gcgttgccga tgaggttctc gagcacgatg
     2221 cggagcaggc gtgggtcggc ggacgcactc acgttcggct ggatgtcgac ggcgacgctg
     2281 cgctcgggtg ctgatttgcg tagatcctcg acgacgagcg tcgcaagcga ggacacatcg
     2341 acgcgctctt tcttgaagtc ggcgcggctg acgcgcgaga gccgcaacag atcgtcgatg
     2401 agctcgccca tgcgctgagc gccagcgcga atgcggttga ggtagtcgtg gcccttcggc
     2461 gagagcttgt cgctctcgtc ctcgacgagc gcgagggaga agcccgcgat cccgcgcagc
     2521 ggcgcgcgca ggtcgtgtga tacggagtag ctgaacgcct cgagctcttt gttcgccgtt
     2581 gccgcggctt gcgcttcacg agcggcttgc tcgcggtatt cgcgcagccg gtctgcggtg
     2641 cgattcgctt cgcgcgcgag atcgccaatc tcgtcgccgg gcggcacatc gatcggggtg
     2701 gcgaagtcgc cgcgtgcgag ccgttctaca ccttcggata ctgcctggag cgagcgcacg
     2761 gtgcggcgga taaggtgcca ggacaggagc gccatgatcg caagcgtcgc gagcgcgacg
     2821 acgatgttga cgaccaatgc agtgcgctgc accgcccgcg ctctcgcgaa tgcgtcggtc
     2881 atgcgcgccc gatcgggagt cgtcgcggcg ttgatcagcg ccaggaagtc gcggcgcgtc
     2941 tgcaccatca cgccgatctc cgtcgtcagc tcggatgccg gccgcccgcg gatcagctcg
     3001 acggatagcg gccgcgccgc ctcggcgaac cggtcgagcg ccgcaagcag cgcatcgggg
     3061 tctgcgccat tacgggcgat cacggagcgg cccgcgtcga tgcgcgcgcg gaaagcagct
     3121 cgcagctgct cggcctctgc aagcttcgat tcttcgccgg catcggccgc atcctcgaac
     3181 gccttcgaga gctccgatgc catccgcttg agatcgccat tgagctctat cagcggcacg
     3241 tagcgcgtct cgataccgac gagctggcgt tcgctgctac ggccgagcgc gagcgtcacg
     3301 acggtgactg cgatcaacgc aaccgcggcg agcgcgagca agagaccgat gcgatggcga
     3361 aagcgaagac gcgccactac tgaaccttga tgactttggc cttgcctgaa tctgcgcttg
     3421 ccggcaggta gccgaccgca cctttgtgcg agagcacgta gctgatcacc gcgtcggtcg
     3481 agctcgcttc gggaggcggc acacctttgc cagagaagat ctgctggttc caatacctcc
     3541 tgagctgcgt cggcgtcttg cgcaggacct tctcgataaa cgcggagcgc acgccgaact
     3601 tcgtcgacag atcgatcgga tgcaccgtcg tgccgctact ccactcggtc gcctttttga
     3661 gataggcatc gcgcaagaag tcgcggctca ctgcgtcgac ggagatgtcg ggatggacga
     3721 tgactttgaa ttcctcttcg gctgcgatcg acgcgctgcc cagcccgagc acgagcatca
     3781 cgacgatcgc caaccggagc gcgcgcatgt cagaacgtca ccgtcgtctt gacgaggaac
     3841 agcccccacc accgctcggg atcgggattc ttcgacgcga acaaatcggc ggtgccatcg
     3901 atgacgtggc cttcgagctt ccacagccag cggtcgttga cgtcgaagcg cagtgtggct
     3961 gacgcgtcgc gctgccaagc ggcgaacggc ttggcgtact tcatcatgtc ccggccgtgg
     4021 cggtcgtcga catcggcgta gagcaccgag tagtacgcgc cgagctcgac cgccggcgac
     4081 aaccgacgcg taacgagcgc gtagaaccgt tcggcctcgt cctcgaacgt cggcaagaga
     4141 gtgcgcagcg tgctggtctg gcgcttgtat gcgcgcgagt actcggcggc gaacgaccag
     4201 tcatcgtgga tgtactctgc ggatgcgatc cacagctggt ctggacgctg ggagatcacg
     4261 agctttccgg tgaagtcagc gggcacgagg ccggccatga tgagcgcact caccgtcgat
     4321 ggatcgagcg tgagcttgaa gtcgatcgag gtcctgagga acgtcccgcc gatgcgtagt
     4381 ccgtcgagcg gcgtatgcca gaagagctgc gcgccagtga cgtacttcgt gtcgatgtcg
     4441 tcgagccgtg cgcccgttag ctcgagcgcg ttctccggaa tattcagcgt gccgagccat
     4501 gcctggtact cgagcgagcc ggcgcaggca ccgagcgagc gttcgccgta gatggagaag
     4561 ccggtctgcg cgaggagggc gctgcgatcg cgcagcggat acacactctg cggcatcagg
     4621 atcgcagtgc gcgccgcgtc gacatccgcg tactcgttat agaggccgaa tggcatcttg
     4681 atgacgccgg cccgcaggcc gagccacgcc ttccagtggt aatcgagata tgcccagtcc
     4741 aggcgcggtg gcaggtcgcg aaatgtcccg acgtcgcgcg cgtagagctg aatgccggcg
     4801 tgcaaacgat cggcgatgtc cttcgagaca ttgaggcccg cctcgaagag ctcgacgctg
     4861 ccgcgcgatg acacgccgag atagtcgttg gcggtcgaga taaacgcgcc ctcgctcacg
     4921 aagccatgaa tcgcgagcgc ctgaccgtcg acttcctcgg tcacgggcgg gggtggctcg
     4981 acgaggaccg ccggggaggc ggcttgcgtt tcgaccggct cgccttccgg ttggggctgc
     5041 gcttcggacg tcggctcggc caacacgccc acgaccacga gcgtgatgat gtgatgagct
     5101 ccccgcaggc ggttcaacgc tagcagcgag cgacgcagcc ggtcctgtcg tgaggtgcag
     5161 ctttttgttg caccgagatc agcgcgtcgg aggcggctcg ttgagcacga gccaatacat
     5221 ccccagctgt cgcgcggcat cgagaaattc cgcgaagtcg accggcttgc ggacgaagct
     5281 gttcgcgccg agatcgtagc tggcgatgat gtcgcgctcc tcggtgctcg atgtgagcat
     5341 cacgacggga agccgccgcg ttcgcgcgtc tgcgcgcatg cgcttcaata catctaggcc
     5401 ggtgagcttc ggcagattga ggtcgagcag cacgaccgtg ggcgcggcat ccgggtcgcg
     5461 acccgcgaac ttaccgctcg cgaacatgta atcgagcgct tcttccccgt ccgtcgcgac
     5521 gatgacctcg ttcgcgatct tcgtgcgctc gaacgcgcgc agcgtgagct cgacatcgtc
     5581 ggggttgtcc tcgacgagga gaatcttctt ggccgcgggc atctcgagca caatagccga
     5641 aatggcgtgc gcaggtcagc gctcgagttt cgcgatccgt cgcagttgct cgagtcgctg
     5701 cccataccgc tgagcgacac gttcgcgcac gcgcgccacg aggtcgggtg acgcgacgtt
     5761 tgggtggccc gcgcgaaacg gcggctcggg gtcgtactcg agctggagct ggatgagctc
     5821 ggcgacgtca cgcccggcga cctcgctcgc gatcgtgaac gcgatgtcga tacccgcagt
     5881 cacgccaccg ctcgtcatgc gattgcgatc gacgacaatg cgcttggcgg tcggaatcgc
     5941 gccgaccagc gagagcagat ccatgaacgc ccaatgggtc gcggcgcggt acccggtgag
     6001 caagcccgcc gcgccgagca gcaacgatcc cgtgcacgcg gatgtgaccc agcgtgcttt
     6061 ctcgccggtc gcatggaccc agtcgagcgt ctcgttgtcg tcggtcgcgt cgacctggcc
     6121 gccgccgccg gggacgaaca agagatcgat cggcggagcc tccgagaacg tggtcgtcgg
     6181 gacgagctgc agcccgccga cctcgacggc gaccgggtcg cgcgtcttcg cgacaacgtg
     6241 cacgattgca ccagggatgc gcgtgagcac ctcgtagggg cccgtgagat cgagctgcgt
     6301 gacccgtgga aacaggagca tgccgatgtt catcgcgtcc tcatcatagg gctctatatt
     6361 gcgaagggca tcatcgaggg ccacggcggc aagatctggg tcaaaaccga gctccacaat
     6421 gggactctgt tcgctttcac gattccgcgc ggtccggcgt agtagcttcg ctggcgatgt
     6481 cgtccgtgcc gtctgcgatc gccgacgcct attcgccaaa gcgcctcggc gccgaaggcc
     6541 atcgcttgat cgatgcgatc gcggagcagc tcgcgagttg gcagcgtcgc gatggggtgg
     6601 cgctgccgtg gcgtgcaccg gcagaagcga gggcgcactg gcgtctccaa gatggtggcg
     6661 ctgacctcgt cgaggatctg cgtcgcatca tgcagagctc gacggcgctc tatcaccctc
     6721 gcgtgatggc gcaccaggtc ccgccgccac tgccgggcgc agcgctcgcg gagcttgtca
     6781 gcgcgcttct caacaacggc atggccgtct acgagatggg gcccgcgtcc gtgccgatcg
     6841 agctcgagac cgtcgactgg atgtgcaagc gactcggcta cggcgacggc gccggcggcg
     6901 tcttcacgtc gggcgactcg ctcggaaatc tcacagcgct gctcgcgatg cgccaggcga
     6961 aagccggctt cgacgtgtgg aagcaaggcg cgcatgctgg ccctccactc gccgtgattg
     7021 tctcgagcga cgcgcattac tcggtcgcac gcacgctccg catcatggga tggggcgacg
     7081 gcggtgcgat cagcgcacag gtcgacgagc gtcatcgcct aacggcggcc agcgtcgaag
     7141 cggcgctcgc aacggcgcgc gaccggcgcg tgatcggcat catcgccgcg gccggctcga
     7201 cggcgaccgg cgcattcgat ccacttcccg agctcgcgga tctcgcggag cgcgaaggct
     7261 tgtggctcca cgtcgatgcg gcacacggcg gcggcgccgc gctatcggcg gctcatcgtc
     7321 accggctcgc gggcatcgag cgcgcagact cgatcgtgtg ggacgcgcac aagatgatga
     7381 tgatgccggc gctcgtcacg gcggtgctgt tcaagcgcgg cagccacgcg tacgaggcat
     7441 tcgcgcagca agcgtcatac ttgttccaag cggcagcgcc ggaggacacg tggtgggatc
     7501 tcggcacgcg cacgctcgag tgcacgaagc gaatgatggc gatcgaggtc tggaccgcgc
     7561 tgcgagtgca tggcgagaag ctgttcggtg acatcatcga tcgcctgtgc gcgctcactc
     7621 gcgcattcgc cgagcgcatc gccgcgaccg atgacttcga gctcgcgctc gagcccgagc
     7681 tgaacatcgt ctgctatcgc cacaagacgg ccgacaaccg cgcgctccgg caacgcgtcg
     7741 tcgaggacgg ccgcttctac atcgtcggca cgcagctgcc gcgcggctac tgtttgcgct
     7801 cgacgatcat gaacccgctc atcgagccga gcgacttcga cgagctgctc gcgcatctgc
     7861 gaacgctatg ccggtagtca cgatcatcgg cgagaacggt cccgcgaccg tcgaggttcc
     7921 cgcgaacacg acgctgttcg atgcgggcgc gaagctcgcg gcgggcatcg acacgtcatg
     7981 cgtcggcaaa ggcacgtgcg gcctgtgtcg cgtgaagatc ctcgccggcg ccgagcatct
     8041 gaacccgttc accgacgagg agcgcaagca cctcggcaac gtctatcaca tcaccaaagt
     8101 gcggctgtcc tgtcgctcgc acgtgagcgg cgacgtgacg atcgaagtcg tgcgtcgcaa
     8161 gaagaagtga gctagcgctt tcccttgccc tcgaacgtca tcgtcgcgcc acccttggtc
     8221 atcttcgaca ccgacttgaa cgcgacttcc ttcttgctcg tccacgtgat cgtgacgtct
     8281 tcggtgactt cgtcgccacc ggggcccatg ccggccttca gcggttcgca ggtgagctcg
     8341 gtcgcggtct tccacgtgca gaggtggtcg tgcacctcgt tcatgttcgt gatgccgatg
     8401 aagtgaacct tcttgtcgta cggattgaag gcgacgacga acagcgcatc catcggaccc
     8461 atcggcgtct tcatcttcga ctcgcacgac acgcccgtct tgccggcgac ttctttgcag
     8521 ttgaacgaca tcttgccggc catgggcttg tccatgccgg gcatgcccgt cgcgctcatc
     8581 gacgcgtcga acttccagtt gcccttgaac gcctcgacgg tcgctttgac ctcggcgggc
     8641 gccgcgacgg gcgccggcgg cggtgcgggc tgcgccgcag gggccttcgg atcggccttc
     8701 ggggccggag gtgtcgccgg agcaccggcc ttcggttgag caagcgcgac ggcgggcacg
     8761 agggagagca gaacgagagc gaatttgttc attagccgcg gagcatcgtc acagctcgct
     8821 cggggaagca agctagtcga acgacgcttt gatcgtctgc ggcgattcgg tgacgttgac
     8881 ggtgatcgtc ttagtcttct tcttgtccgt cggatgcacg agcttgatct gctgcgagcc
     8941 aaccggcaga tgaaacgcct gcggcccttg catcgtgcgc gtacggccga ggttcttgcc
     9001 cttgaagaac gcttcggcat acgacgcgct gccggtgacc tcgatcatca ccgtgcccat
     9061 cttgtcgagc tccttgaggt cgcgcttgat atcgacgggc ttgccgagcg cgagcgtgat
     9121 cttgatcttc accgtctcgt agccctgttt cgtgatcacg aggtcgcccg atttcggctc
     9181 gaggccatcg cggttgagcg gcgtctcgcc gaggagcttg ccgcccatcg cgacctgcgc
     9241 gccgctcggg ttcgtctcga cgtgcagctt cgcgctcgcc ttctcgagcc gcgggctgat
     9301 gcgcagcgtc tcgttcggca gcaccgtgct ctcggtgtcg tacgggttgt agccggggag
     9361 ctcgatgtgg atcttgaact tcgcgttcgc gctgaccttg ttgcggatca gcttcttcgg
     9421 atcgtgcgac gtcttgccga gctcgtagac cttgccgttc tcgtcggtga gagtgacgct
     9481 cgcgccgtcg ggatccggat cgatgacgaa gaagccgacg ggaatctcgg tgccgccgtc
     9541 attagccgtc acgaccgtcg tcgcggctgg tttctgcgtc agcttccaga tcgcaaaccc
     9601 gccgccgccg aggatgacga gcaggacgag cagcaagagg ccgcgtcgcg acggtggcgg
     9661 ctccggctgc ggctctttgc gaagcgggcg cgttggatcg gctttcggca ccggtgccgt
     9721 cggtgcgggc ggcgcggctt cgtccgtgcg ccgatcgcgg tcgtagcgcg catcgaggag
     9781 ctgatgcatc gccgaatccg gcgacgaggg gcgattgttc ggatcgtcct gcagcgcatc
     9841 gacttggccc gtcgcgcgct ttgcacgtgg caccgcaaca gctgtggggt tgtcgacttc
     9901 ttcgagccga gagaggccgt cgttgccgac tttgcggaac aggcccgtcg acgtcttgct
     9961 ctcgccggtc tccgtcgcga cg
//
LOCUS       DATLBZ010000076         4233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311 k141_63570,
            whole genome shotgun sequence.
ACCESSION   DATLBZ010000076 DATLBZ010000000
VERSION     DATLBZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63570"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /gene="ugpC"
                     /locus_tag="VMZ53_04105"
     CDS             complement(<1..962)
                     /gene="ugpC"
                     /locus_tag="VMZ53_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HUS27660.1"
                     /translation="MSDIALQGIRKVYEGQSRAVIPGLDLEIPSRKMVVLVGPSGCGK
                     STTLRMVAGLEDPTAGKIFIDGRDVTNLPPAERDIAMVFQSYALYPHMTVFENMAFGL
                     RIKHVAESEIKKRVHEVAASLGLDTYLERRPKALSGGQRQRVAIGRAVVRQPKVFLFD
                     EPLSNLDAKLRGEMRREIARIHQMSNATGMYVTHDQIEAMTLADMIVVLKDGVIQQIG
                     TPVDIYEKPANRFVAGFFGTPSMNFLSADLAQTGERQAARGPGFEIPLALTPSLRPAQ
                     SAVDRIVVGIRPERLSLERYDNNVGLSGEVAMREVLGAEVVLHL"
     gene            1200..1808
                     /locus_tag="VMZ53_04110"
     CDS             1200..1808
                     /locus_tag="VMZ53_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27661.1"
                     /translation="MKRELAAKGKPATPEKHEMPIPERFRVARGDGDVRVSWRWLEAK
                     HFYSLFFVIAWDAFLIFWYTQAVPTGELLAIVFPIAHVAVGVGMTYSTLSGFLNRTTL
                     RAHDGTLRIEHGPLPWRGNATLNRDEIRQLYVFTHTTTDSDNDTSTTYQLCALLANGR
                     ERKLVKGLREQNEAAYLEDMLENALGIANASVPGETRISRAS"
     gene            1925..3463
                     /locus_tag="VMZ53_04115"
     CDS             1925..3463
                     /locus_tag="VMZ53_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27662.1"
                     /translation="MSSVGAQIGAYKILDQLGEGGMGTVWRAEHMGIGRKAAVKMLHG
                     EYSHRAEIVQRFFNEARAATQISDPGIVQIFDFGTTPEGYAYIVMELLVGEALDRRLQ
                     RLGKLTVHDALRIMRQVSSTLGAAHAQGIVHRDLKPENVYMVRDPEVPGGERAKILDF
                     GIAKLSDNAGIKTQTNAIMGTPMYMSPEQCRGAGGIDARSDVYALGCLMFALITGLPP
                     FVAEGHGELIVMHLTQPAPLMSSRAQHVAPAVDAIAARCLAKNPAERYASGTELAHAI
                     GMLITSLSQPGPGVMAPNTATHQAPTIALSSEQQTTLSGAAAQTPGTMTTQVVTRSKV
                     PLIAGAIAVLGIAGGIGFLAVNGGGDKKTTVASESPTTPTPPAPKPEAPKPEAPKPEA
                     PQPEAPKPEAPKPEAPKPEAPKPEAPVAVEKVSIPITSTPAAQLFVGDEKTARGTTPY
                     TLELEKGGKPVAIKLVAAGYKAQKRTVAPTETNVAVKLVKAVAATTTSGDSDDTMNPF
                     AKKK"
     gene            3466..>4233
                     /locus_tag="VMZ53_04120"
     CDS             3466..>4233
                     /locus_tag="VMZ53_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS27663.1"
                     /translation="MMRKLGIAVVTSLLFSSAGVALAQNADQQKVAEQEFIQGDKAYN
                     LGRFDEAVDHFTKAYEAWPQPEFLYNIAQSYRLGGNCKQAVYFYKRFRSLKEKELSAK
                     KKEEVDKFIKQLADCAAKADSGGNVQPQSLDRPTMPTTTTATTTTTTPTTTTTPKPNT
                     TTPTTTSTTPKPPTTTPTTTSTTPTTTTPTTTTPTTTTPTTTTTPTTTSTTPTTTTTT
                     TTAQLENPDEEPEEQEPSVRAQTGTGATMISARAEGGV"
BASE COUNT          860 a         1345 c         1394 g          634 t
ORIGIN      
        1 tcgagatgga ggaccacttc ggcgccgagg acttcgcgca tcgcgacttc gccggagaga
       61 ccgacgttgt tgtcgtaccg ctcgagcgag aggcgctcgg ggcgaatgcc gacgacgatg
      121 cgatcgacgg cggattgtgc ggggcgcagc gagggagtga gtgcgagcgg gatttcgaag
      181 cccggaccgc gcgcggcctg acgctcgccg gtttgcgcga gatccgcgct caggaagttc
      241 atcgacggcg tgccgaagaa tccggcgacg aagcggttgg ccggcttctc gtagatgtcg
      301 accggcgtgc cgatttgctg gatgacaccg tctttgagca cgacgatcat gtcggccagc
      361 gtcatcgctt cgatttgatc gtgcgtgacg tacatccccg tcgcgttcga catctggtga
      421 atgcgcgcga tctctcgccg catctcgccg cgcagctttg cgtcgaggtt cgagagaggc
      481 tcgtcgaaca ggaagacttt cggttggcga acgactgcgc gaccgatcgc gacgcgttgg
      541 cgttggccgc ccgagagcgc tttcggacga cgctcgagat acgtgtcgag accgagcgac
      601 gcggcgactt catgcacgcg ctttttgatc tccgattcgg cgacgtgctt gatgcgcagg
      661 ccgaacgcca tgttctcgaa caccgtcatg tgcgggtaga gcgcgtagct ctggaacacc
      721 atcgcgatgt cgcgctcggc gggcggtagg ttcgttacgt cgcggccgtc gatgaagatc
      781 ttgccagcgg tcggatcttc gagacccgcg accatgcgca gcgtcgtcga cttgccgcag
      841 ccgctcggcc cgacgaggac gaccatcttg cggctgggaa tctcgaggtc gagacctgga
      901 atcaccgcgc gcgactgacc ctcgtagacc ttgcggatcc cttgcagcgc gatgtcggac
      961 acggcccgcg cgaggagcaa gcgtcacgcc aggtgcaagt gcccgtcgag aaaggcagga
     1021 gcggcgctca tgttgaggtt ggcaacaagg gccaaacggc cggcgacggc ttgtgcaacg
     1081 ccttccgcgc cttaccgtgg aggcgaacaa cgaggagcac cgaaaacagc ttgagttacc
     1141 gaaacgatct ggaggcggca cacgagcggg tggcggcatc ggagcgcgaa gtggcgcgct
     1201 tgaagaggga gctcgcggcg aaggggaagc cggcgacgcc cgagaagcac gagatgccga
     1261 ttccggaacg gtttcgcgtc gcgcgcggcg atggcgatgt ccgggtcagc tggcgctggc
     1321 tcgaggcaaa gcacttctac tcgctgttct tcgtgatcgc gtgggatgcg tttctgatct
     1381 tttggtacac gcaggcggtg ccgacgggcg agctgctagc gatcgtgttt ccgatcgcgc
     1441 acgtcgcagt tggcgtcggc atgacgtaca gcacactctc cggttttctc aatcgcacga
     1501 cgctgcgcgc tcacgatggc acgttgcgga tcgagcacgg cccgctgccc tggcgcggga
     1561 acgcaacgct gaatcgggac gagattcgtc agctgtacgt gttcacgcac acgacgaccg
     1621 acagtgacaa cgacacgtcg acgacgtacc agctctgcgc gctgctcgcc aacggtcgag
     1681 agcggaagct cgtgaagggt ctacgcgagc aaaacgaggc cgcgtatctc gaggatatgc
     1741 tcgagaacgc gctcggaatt gcgaacgcga gcgtgcccgg cgagacgcga atttctcgcg
     1801 cgagctgacc aaccgtaagt gggccgacgt cccatacggc ggatcggccc gaaagatcgc
     1861 ggaaatcgtt gatacgacgg aggtttcatt tgaattccga ccgggggccg cttacaacaa
     1921 aatgatgtcg tctgtgggtg cgcagatcgg ggcgtataag attctcgatc agctcggcga
     1981 aggcggaatg ggaaccgtct ggcgcgccga gcacatgggc atcggccgca aggccgccgt
     2041 gaagatgctg cacggcgagt actcgcatcg cgcggagatc gtgcagcgct tcttcaacga
     2101 ggcacgcgcg gccacgcaga tcagcgaccc gggcatcgtg cagatcttcg acttcggcac
     2161 gacacccgag ggctacgcat acatcgtgat ggagctcctc gttggcgagg cgctcgatcg
     2221 tcgtctgcaa cggctcggca agctcaccgt gcacgacgcg ctgcgcatca tgcgccaggt
     2281 ctcgagcacg ctcggcgcag cgcacgcgca aggcatcgtc catcgcgatc tcaagcccga
     2341 gaacgtgtac atggtgcgcg atccggaagt gcccggcgga gaacgcgcaa agatcctcga
     2401 cttcggtatc gcgaagctca gcgacaacgc cggcatcaag acgcagacga acgcgatcat
     2461 gggcacgccg atgtacatgt cgccggagca gtgccgcggc gctggcggca tcgacgcgcg
     2521 ctccgacgtc tacgcactcg gctgcttgat gttcgcgctg atcaccggat tgccgccgtt
     2581 cgtcgcggaa ggtcacggcg agctcatcgt catgcacctg acgcagccgg cgccgctcat
     2641 gtcgtcgcgc gcgcagcacg tcgcgccggc ggtggacgcg atcgctgcga ggtgcctcgc
     2701 gaagaatcct gccgagcgtt acgcgtcggg cacggagctg gcgcacgcga tcggcatgct
     2761 gatcacgagc ctgtcgcaac caggtcctgg cgtcatggcg ccgaacacgg cgacccatca
     2821 ggcaccgacg atcgcactga gcagcgagca gcagacaacg ctgtcgggcg cggccgcgca
     2881 aacgcccggc acgatgacca cccaagtggt gacccgcagc aaagtgccgc tcatcgcagg
     2941 cgcgatcgcc gtgctcggca ttgcgggcgg cattgggttt ctcgcagtga acggcggagg
     3001 cgacaagaag acgacggttg cgagcgagag cccgaccacg ccgacgcccc cagcaccgaa
     3061 gccagaagcg cccaagcccg aggcacccaa gcccgaagcg ccgcagccgg aggcgccgaa
     3121 gcccgaagcg cccaagccgg aagcgcccaa gcccgaagcg ccgaagcccg aagcgccggt
     3181 cgccgtcgag aaggtctcga ttccgatcac gtcgacgccg gcggctcaac tgttcgtcgg
     3241 cgacgagaag accgctcgcg gcacgacgcc gtacacgctc gagctcgaga agggcggcaa
     3301 gcccgtcgcg atcaagctcg tggctgctgg ctacaaagct cagaagcgca ccgttgctcc
     3361 gaccgagacg aacgtcgcgg tgaagctggt gaaggccgtg gcggccacga ccacgtcggg
     3421 cgacagcgac gacacgatga acccattcgc gaagaagaaa tgatgatgat gcgcaagctc
     3481 ggcattgcag tcgtaacgtc gttgttgttc tcgagcgcgg gcgtcgcgct tgctcaaaat
     3541 gccgaccagc aaaaggtcgc cgagcaggag ttcatccagg gcgacaaggc ctacaacctc
     3601 ggccgattcg acgaagccgt cgaccacttc acgaaggcgt acgaagcctg gccgcagccg
     3661 gagttcctct acaacatcgc gcagtcgtat cggcttggcg gcaactgcaa gcaggccgtc
     3721 tacttctaca agcggttccg ctcgctgaag gagaaggagc tctccgcgaa gaagaaggaa
     3781 gaagtcgaca agttcatcaa gcaacttgcc gattgcgccg cgaaggccga cagcggcggc
     3841 aacgtgcagc cgcaatcgct cgatcgtccg accatgccga cgaccacgac ggcaacgacg
     3901 accacgacca cgccgacgac gaccacgacg ccgaagccga acacgacgac tccgacgacg
     3961 acgtcgacga ctccaaaacc gccgacgacg actccgacca cgacgtcgac gactccgacc
     4021 acgacgactc cgaccacgac gactccgacc acgacgactc cgaccacgac gacgactccg
     4081 acgacgacgt cgacgactcc gaccacgacc acgacgacga cgacggcaca gctcgagaac
     4141 cccgacgagg agcccgagga gcaagagcct tcggttcgcg cgcagacggg aacgggcgcg
     4201 acgatgatct ctgcgcgtgc agaaggcggc gtt
//
LOCUS       DATLBZ010000077        19549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5282592, whole genome shotgun sequence.
ACCESSION   DATLBZ010000077 DATLBZ010000000
VERSION     DATLBZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19549
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5282592"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VMZ53_04125"
     CDS             complement(<1..367)
                     /locus_tag="VMZ53_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27664.1"
                     /translation="MRIDTVLTDVRVFPLLEITREQLEQAAGAPIVQVERVDGGLTNT
                     IHKVTLEDGDVLGIKHYAGGRDWFETELTTLTLLHGTVPVPDVVQANEERLVIAYRWI
                     DGITLHELRKQGQFDAFQSL"
     gene            complement(397..1974)
                     /gene="rny"
                     /locus_tag="VMZ53_04130"
     CDS             complement(397..1974)
                     /gene="rny"
                     /locus_tag="VMZ53_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HUS27665.1"
                     /translation="MEPIYILAAVALGLVAGGVLGTRLGRARGPNDAEKVAEEEAAKI
                     RSAAQAEVDAIKKAAEVEGKEAARKRKSELDEELKSRKTELQKREEALSAKERDLDKQ
                     RKDAERRAGEVEKKERSVDGKIKQADAAVEKAEAAQAEARKKLEQISGLTEEAARKKL
                     EEEVRGQALQSAALEIKRIEDEASKEATARAKTIVATAIQRFASEFVHERTVAVVPLP
                     SDDLKGRLIGREGRNIRALEAATGIDMIIDDTAEAITISCFNPVRREVARLAIGALVA
                     DGRIHPTRIEECVRKAEKEVDKLCKEAGEQAVFDLGLHRVHPELVRHLGRLKFRQSYA
                     QNVLLHSIEVGYLAGLIAAELGANVKLARRAGLLHDVGKAVDHEQEGPHADVGAQLCK
                     KHGESPKVCQAIAAHHGDEGAGARPQALLDHIVAAANSLSGARPGARREQLASYIKRL
                     DDLEAICRRFNGVERAFALQAGREIRVMVMNDQVDDAQAIVMSKEIARAIETEATYPG
                     QVRVVVVRETRASDYAR"
     gene            complement(1974..2561)
                     /locus_tag="VMZ53_04135"
     CDS             complement(1974..2561)
                     /locus_tag="VMZ53_04135"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HUS27666.1"
                     /translation="MLGSLAMQKPITRRTVLLRRDTMPELARIAAGDLIADSVMQLVA
                     RFPKGEVIGLYAPKGTEVDTARIDAFARARGLRIAYPRVADNTKLLAFHETTIEALEE
                     GHFSLREPRADAPSLELSAIAAFIIPGLAFDRHGGRVGWGRGYYDVTLAAANPQALRI
                     GLAFECQLVEQVPRDPHDMHLHYVVTEAAVYRAAD"
     gene            complement(2619..3647)
                     /locus_tag="VMZ53_04140"
     CDS             complement(2619..3647)
                     /locus_tag="VMZ53_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27667.1"
                     /translation="MLVLMLLVASAHADPARCAVVGSSVTLQEITVAVPHEEPFQLGL
                     QAVPAIAHIPARCGAPLELDVRGAIAFTASRDLVRLRLTRKITTPDGLVTLERGAQVI
                     DACMRGDVVVASAILEEDDVLEGEHKPADQFVRDVEVPCDALSLDVIESTTDDALPVD
                     DEEVVHRWELRGEASKIALYKQPNMKASARLLESPSCSGCIKLEEVKRTKHWLLVAAY
                     AYGGSVEARGWVRRSLVKQIPGDVGIYGGLMCTGDHGGGMFGEGAFPGAVEREGTVRM
                     GTAVYLGDGTGMWGTFSRDEHVKVRFVPGSWWAELRVVPGLDGDPMGSPWLHGNVRLD
                     TVTLDPTP"
     gene            complement(3715..4500)
                     /locus_tag="VMZ53_04145"
     CDS             complement(3715..4500)
                     /locus_tag="VMZ53_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS27668.1"
                     /translation="MIRAARPDDYDACVRLHAELGVPDPVPERAEWVATNMQRIAVVT
                     DEEDQAIGYMSWRGYGPLAHVMHLAVDPAHRGRRLGEQLLVHARDEAKAAGCTRWYLN
                     VKRDNAPALKLYERVGLRIELESFAMKLPWANVPRVAARHRLADTSADDATIERLFNV
                     PADRVASFRARGSFRLVTLRDDADALLGFAAFSPGFPGAATFCTRSPNLAIPLLEAMR
                     HYADPQFDYVRVTIEGDRPLAEAVLALGAELTFEILRLSATIP"
     gene            4621..5382
                     /locus_tag="VMZ53_04150"
     CDS             4621..5382
                     /locus_tag="VMZ53_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27669.1"
                     /translation="MRSFKALLFAAVVAGCAGSGAVRYSATAEVSTPEMVEVEPGVMV
                     VADYNEPVFYSEGVYWRYESGTWYRSPYYNRSWVRISTPPLAVRRIERPEIYVHYRAG
                     ARDRREARAYQPQQNPEVRDHRTYPQPAPAPMPAPAPAPDVRDHRTYTPAPTPLPPSP
                     PDVRDHRDDHREDHRDDRRDDHRDDRQDRREEKRDDRQDRREEKLERKEEHREDKAER
                     REDKQERREDKAERREDKHEHRDDKHDDHNKHGHH"
     gene            5529..5768
                     /locus_tag="VMZ53_04155"
     CDS             5529..5768
                     /locus_tag="VMZ53_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27670.1"
                     /translation="MVDTKSIWDDIKRMAQELEVQMHLAEMEARERWTALKPRIGELQ
                     KDMSDTGKKVNEVVEKELSSVGAALRKLRDDIVKR"
     gene            5816..7744
                     /locus_tag="VMZ53_04160"
     CDS             5816..7744
                     /locus_tag="VMZ53_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUS27671.1"
                     /translation="MRLLLITPPMTQLNTPYPATAYLAGFLHQHASRLDLEVHQSDAA
                     LELFLRVFSRKTLQEILDELTARAAEVDEDDRAEMPPSIASFLQHGPRYVATVDAVVR
                     FLQGRDPGLALRIVTRELLPEGPRFAAIAQGPGASEADPLAWAFGGLGTADRAKHLAS
                     LYIDDLADMIRDGVDPQFELSRYGERLAASAASFDPLHESLEGDPSLIDELLDEIARE
                     LYERYKPDVVGLTAPFPGNVYGAFRIARSIKASAPRTTLVLGGGYVNTELRELSEPRV
                     FDYFDFITVDDGEAPTLALVEHLRDTGKPLLRTFVRRDGKVTFQTDDALHDIPLRDAG
                     TPTYRDLPLDRYLSLFEMLNPMHRLWSDGRWNKLTIAHGCYWKQCTFCDITLDYIARY
                     DRAPADLLVDRIEQLVAETGQTGFHFVDEAAPPAALRALAETIIARKLTITWWGNVRF
                     EKAFTPELCKLLAESGCVAISGGLEVASDRLLELMRKGVTVEQVARVTRAFTDAGIMV
                     HAYLMFGFPTETAQETLDSLERVRQLFAEGCIQSAFWHRFAATAHSPIGQKPELYGIK
                     PRKPPTITFAKNDVEFDDPTGTDHDFFAPGLRKAIYNYMHGLGLDADPRTWFGKRTKL
                     AKSTVPPDLVRRALRSAI"
     gene            complement(7951..8583)
                     /locus_tag="VMZ53_04165"
     CDS             complement(7951..8583)
                     /locus_tag="VMZ53_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27672.1"
                     /translation="MVPPLDVSFGVEPDEPEVPDEPLVPVPDEPLVPVPDELDVGFFF
                     FVVVGLVVDVPVEPLVELSVEPLVELSVEPPLDEPLMSVPVPDDPLVPEEPAAPELPV
                     VEFPEEPPVPVVEPVPFIEPLVPVEPPVPFIEPPVPFIEPPVPSIEPPVPLTPEGSSY
                     WPVPDVPPMPVPRVRMSPERAAAPPPLVALISGDVVGVVAAGVVSRRVSW"
     gene            complement(8727..9185)
                     /locus_tag="VMZ53_04170"
     CDS             complement(8727..9185)
                     /locus_tag="VMZ53_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS27673.1"
                     /translation="MQIKPGDLTDERVVDLLREHLAGMHASSPPGSVYALDLSGLQHP
                     SIAFFTVWDGDDLLGCGALKHVDAQTGEIKSMRTTARHLRRGAGAFMLKHLLSLARSR
                     GYTRVCLETGSGPAFEAALAMYRKHGFVDGPAFGDYVATEFNQFLHLDLR"
     gene            complement(9193..10008)
                     /gene="rluF"
                     /locus_tag="VMZ53_04175"
     CDS             complement(9193..10008)
                     /gene="rluF"
                     /locus_tag="VMZ53_04175"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: protein motif:HMM:NF007784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HUS27674.1"
                     /translation="MRLNKYLSESGACSRREADTFISEGRVTVNGAPAALGTQVNEGD
                     DVRLDGDRVGTARKKVKPVYIALNKPVGITCTTERHVAGNIVDFVDHPERVFPIGRLD
                     KDSEGLILLTNDGDIVNEVLRAEHNHEKEYVVAVDKPFDADFVTKMSSGVRLSDATTK
                     PCKVEKLGAKVFRIVLTQGLNRQIRRMTEAFGYTVEALQRVRIMHIKLGQLPLGRWRN
                     LSDQEVAALFPKKPAQQPQSPGPSRPHQQHGRPQQQHQQPRKPGHNPRHQRRR"
     gene            10045..10710
                     /locus_tag="VMZ53_04180"
     CDS             10045..10710
                     /locus_tag="VMZ53_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS27675.1"
                     /translation="MAKTDYAAHDARYRQLREEGAPGWDTADGYREREAEYAWAIDAI
                     DAIGRPGNRLLELGCGAGNATPWFVERGFEVTGIDISPAAIAWANERGIAGARFVVGD
                     LVASIPGQYDIAVDGHCLHCIIGDDRARFLANVRNALAPGGSFFVATMCGEITLPKLR
                     ASFDPATRCQVVDGIAYRYIGDASEILDEVRAAGFEVVASTINARKDDNDQDNLWAIA
                     RKR"
     gene            complement(10717..11664)
                     /locus_tag="VMZ53_04185"
     CDS             complement(10717..11664)
                     /locus_tag="VMZ53_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HUS27676.1"
                     /translation="MIPEPHREAVTHALHAAFGVAEPAAIEPLTEGMSQSLVYRLEVR
                     GRSYVLRIHLNTLGSDPTRHFIYMQHAADAGIAPRVYFASTTDKIVITDFVERKPFPP
                     DMVAHLAPTLRRLHALASFHPSIDQFTVIEKFVGQFDAFDVGDLFDRFADVAAVYPRK
                     LDHVSSHHDLKPENIAFDGERLLLLDWEAAFLDDRNFDLVIPANFFVRDEATLERYLT
                     LYYGSMPSPGQRARFELIRIAEHVFYATFLAAIAKRGGVVDDGAPAEDFDAFHDYLLG
                     GAIVREPLQQLRYAKAHAAKGLQRVHSPRFREWLSRALD"
     gene            complement(11762..12493)
                     /locus_tag="VMZ53_04190"
     CDS             complement(11762..12493)
                     /locus_tag="VMZ53_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27677.1"
                     /translation="MRRALVLVLVAACGTSDMNDVGDDTGSGSGEGSGSGSGSGSEST
                     SCTNYTPPDLPDSVFVNRDVEAIAKRDALTHFSVSGSTSTNSAGKITRFSHDETGSEN
                     DFTVDFSYNSANNLTRVSYDGYGSMNDTTDDFSYNSSQKLSRWSHDAYGSMNDFTDDF
                     SYNSNNKLSRFSHDAYGSMADITDDYSYNSRKDISRYSEDLYGSAEDITENFSYDSYG
                     RLTRWTLDGGGQHLTSDFSYGACQH"
     gene            complement(12596..13549)
                     /locus_tag="VMZ53_04195"
     CDS             complement(12596..13549)
                     /locus_tag="VMZ53_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27678.1"
                     /translation="MSQNDRLSKARADKRELAWVRRRLAETREERDRLAAQLAIAEQA
                     RDYEQADVDALTKGIGTFFRRVMADHADLSKEQQELAAAQLLCDELTDELRSIDADIA
                     QLTTRENAVRDADARYAQVLAEVEAEVREQGELNEELDALVAAESELTSSRREVREAM
                     TIGVDVQKSFIAVVNYARELEPRPQDEVEREGLVHAMMGWTRESYTGLCGALAAAQQG
                     LRRFEQACHTLQTTLPPGIDFDLRLEPLPETNRFILRDVMWTNRRLIEGILPEVSRVS
                     GSLSTAIGELKKRDSQLAQALAECTRVRAHLLDPVLPSDLN"
     gene            complement(13546..14136)
                     /locus_tag="VMZ53_04200"
     CDS             complement(13546..14136)
                     /locus_tag="VMZ53_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27679.1"
                     /translation="MISTPLVRLLRSVRDRTEMVDQQLDALMSSASAEGYVRYLCDVY
                     GFTAGIAPTVGASLYASARIHARAATLVFELHAWGASPDDIAEIGRLAQRAFDQTPRT
                     EIEALGALFVVERAWLHAVRVRSRLTAEASRNPDAERKTWLDLGRHVSRVVTNDIEMR
                     TLVAATSEALDSLGDWALVKSHVRSDEVRERYRLSP"
     gene            14300..14977
                     /locus_tag="VMZ53_04205"
     CDS             14300..14977
                     /locus_tag="VMZ53_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27680.1"
                     /translation="MIPWKVLATVDTSEGPLQLRQRGAREFLITIGGRVLMTSSERTS
                     ELAVATLACKELAGHKAPRILIGGLGMAYTVRAALDALPKRAQVMVAELTPEVETWCR
                     GPLAPLTNGAANDPRVRIVIRDVARVIEEAPPGRFDAIVLDLYEGPHAASRRENDPFY
                     GRVALERSYAALSPGGVLAIWSEESNTVFKRRMIDAGFDTTLHHPGGSRSYVVYLGRR
                     ESPRERR"
     gene            complement(15359..19117)
                     /locus_tag="VMZ53_04210"
     CDS             complement(15359..19117)
                     /locus_tag="VMZ53_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS27681.1"
                     /translation="MLDVPQHDGELIARIVPSGGEMGRRMRELDWSNVAVGRPSTWSP
                     SFRSALSICLGSRFPIALYWGDDLTLIYNDAWSPILGTKHPQALGNAARVVWPEIWDA
                     IGPLFSTVLATGESSYQEDGLLLMNRHGYTEECYFNFTFTPVRGDDGRIEGVFNAVNE
                     TTFRVISERRASVLRNLAEHAISDRSVDDVAERVVKTLGAARFDATFSVIYLADESGG
                     LRRVGWTGLDDAEAIAPAYIAAEHVAESRWRFGDVRRSGKVEIVDRLDELPVPLSGGA
                     WPEPTRSAMVAPIRSTAAPDSWGFLIIGASPRRAVDREYEEFAERAAATVTRVVANAL
                     TLEAERRRADELAELDRAKTAFFSNISHEFRTPLTLMLGPQQDALESPANSLGGADLQ
                     AVHRNTLRLLKLVNSLLDFARVQAGRATAAYEPVDLAALTSDLASAFRSAIERGGIRF
                     EVDCPSLQEPIFVDREMWEKIVLNLLSNALKFTFEGSIRISQRIVGDHVEVEVEDSGV
                     GIPEHEMPRLFERFHRIEGTKARTHEGSGIGLALVHDLVQLHGGTIVASSVPDKGTTF
                     RITLPRGSAHLPASHIKANVRAHDMESTAKPFVVEALRWVDPSASAPRPVAANSTTHV
                     LIADDNADMREYLARLLGQHWSVDAVADGALALAAVRRRRPDLVITDLMMPNMDGVSL
                     LHALRADQATRDLPVIALSARAGEEARVEGLRAGFDDYVVKPFSARELVVRVETQLAK
                     MQVRAVTELHNRRLANVFANTPVGIAILRGPEHRFEFVNASYAALVHDRSVLGKTIRE
                     AFPDLAGQGSYELLDAVYTSGEPQSVRSREIVLVRQTDGVAERRFFDTVYQPLRDEAG
                     AVDGIVVVVFEVTELARARRLAESANRAKDEFMAILGHELRNPLAPILTAVHLMQLRG
                     DTSLEKERSVIERQANHLVRLVDDLLDVSRITTGKVELKKQPIEVGEVIAKALEMASS
                     MLEQRKQQLTLDVPSRGLVVNGDPTRLAQVFSNLLTNAAKYTEPGGVITVTAARQDAR
                     IVIRIRDTGIGISAEMLPRVFELFTQERQALDRANGGLGLGLSIVKNLVAMHGGLVEA
                     SSAGRGCGSEFVVTLPAVNQVSAAVTNASSTNRDAEAQSIRMRVLVVDDNVDAAELLA
                     ESLEMMGYTTRTAHDGPSALVVADDFKPEIAVLDIGLPAMDGYELARLLRGQPALAGL
                     RLVAVTGYGQETDRRAAQESGFNAHLVKPVDMDRLRTVLRQVAPTGHA"
     gene            complement(19255..>19549)
                     /locus_tag="VMZ53_04215"
     CDS             complement(19255..>19549)
                     /locus_tag="VMZ53_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27682.1"
                     /translation="RARGFAFVTMSTPDEAAKAMQSMDGQTLDGRPLRVNEAEQRQQR
                     GGGGGGGGGYRGGGGGGGGGGGDWGGGGGGGDDRGGGRGGRGGRGRGGGGDRW"
BASE COUNT         3389 a         6365 c         6381 g         3414 t
ORIGIN      
        1 cgagcgactg gaacgcgtcg aattggcctt gcttgcgaag ctcgtgcagc gtgatgccgt
       61 cgatccaacg atacgcgatc acgaggcgct cttcgtttgc ttgcacgacg tccggcaccg
      121 ggaccgtgcc gtgaaggagc gtgagcgtcg tcagctccgt ctcgaaccaa tcgcggccac
      181 cggcgtagtg cttgataccg agcacgtcgc cgtcttcgag cgtgactttg tggatcgtgt
      241 tcgtgagccc gccatcgacg cgctcgactt gcacgatcgg tgcgccggct gcttgttcca
      301 gttgctctcg agtgatctcg agaagcggga acacgcgcac atcggtgagg acggtatcga
      361 tgcgcaccgg gcgtccagaa tatcaggtcg ggcgttctat cgagcgtagt cggaggcgcg
      421 ggtttcgcgg acgaccacga cgcgcacctg accgggatag gtagcttcgg tctcgatcgc
      481 gcgcgcgatt tccttcgaca tcacgatcgc ttgcgcgtcg tcgacctggt cgttcatcac
      541 catcacgcgg atctctcggc cggcttggag cgcgaacgcc cgctcgacac cgttgaaacg
      601 cctgcagatc gcttcgagat catcgaggcg cttgatgtag gaggccagct gctcccggcg
      661 tgcaccgggg cgcgcaccgg agagcgagtt cgcggcggcg acgatgtggt cgagcagcgc
      721 ctgtggacgc gcgccggcgc cttcgtcacc gtgatgggcg gcgatcgctt ggcagacctt
      781 cggcgactcg ccgtgcttct tgcagagctg cgcaccgacg tcggcgtgcg gaccttcttg
      841 ttcgtgatcg acggccttgc cgacgtcgtg caggagaccc gcgcggcgcg cgagcttgac
      901 gttcgctccg agctcggcag cgatgaggcc tgcgaggtag ccgacctcga tcgagtggag
      961 cagcacgttc tgcgcgtacg actggcggaa cttgaggcgt ccgagatggc gcacgagctc
     1021 gggatgcacg cggtgcaagc cgaggtcgaa cacggcctgt tcgccagctt ctttgcagag
     1081 cttgtcgact tccttctccg ccttgcgcac gcactcttcg atgcgcgtcg gatggatgcg
     1141 accatccgcg acgagagcgc cgatcgcgag ccgggcgact tcgcggcgca ccgggttgaa
     1201 gcaggagatc gtgatcgctt cggcggtgtc atcgatgatc atgtcgatgc ccgtcgcggc
     1261 ttcgagcgcg cggatgttgc gaccttcgcg gccgatgagg cggcccttta gatcgtcaga
     1321 cggcagcggc accacagcga cggtgcgctc gtgcacgaac tcgctcgcga agcgctggat
     1381 cgcagtggcg acgatcgtct tggcgcgcgc ggtcgcttcc ttcgacgcct cgtcttcgat
     1441 gcgcttgatc tcgagcgcgg cggattgcag cgcctggccg cggacttcct cttcgagctt
     1501 cttgcgcgcg gcttcttcgg tgagaccgct gatctgctcg agcttcttgc gagcctctgc
     1561 ctgtgcggcc tccgcctttt cgaccgcggc gtccgcttgc ttgatcttgc cgtcgacgct
     1621 gcgctctttc ttctcgactt cgccggcacg gcgctcggca tcttttcgct gcttgtcgag
     1681 gtcgcgctct ttggcagaga gtgcttcctc acgcttttgc agctcggtct tgcgcgactt
     1741 gagctcctcg tcgagctcgg acttgcgctt gcgcgcggcc tccttgcctt cgacttcggc
     1801 cgccttcttg atcgcgtcga cctcggcttg tgccgcggag cgaatcttcg cggcttcttc
     1861 ttccgcgacc ttttcggcgt cgttggggcc gcgcgcgcga ccgaggcgcg tgccgagcac
     1921 gccaccggcg acaaggccga gcgcgaccgc tgcgagaatg taaatgggtt ccatcagtcc
     1981 gccgctcggt aaaccgcggc ctcggtgacc acgtagtgaa ggtgcatgtc atgcggatcg
     2041 cgcgggacct gctcgacgag ctggcactcg aacgcgagac cgatgcgaag cgcttgcgga
     2101 ttggcagccg cgagcgtgac gtcgtaatag ccgcgacccc agccgacgcg accgccatgg
     2161 cgatcgaagg cgaggcccgg aatgatgaac gcggcgatgg ccgacagctc gagcgacggt
     2221 gcgtccgcgc gcggctcgcg cagcgagaag tggccttctt cgagcgcttc gatcgtcgtc
     2281 tcgtgaaacg cgagcagctt cgtgttgtcg gcaacgcgcg gatacgcgat gcgaagacca
     2341 cgcgcgcgcg cgaatgcatc gatgcgtgcg gtgtcgacct cggtgccctt cggcgcgtag
     2401 aggccaatga cctcgccttt ggggaaccgt gcgacgagct gcatgaccga atcggcgatc
     2461 agatcgccgg ccgcaatgcg cgcgagctcc ggcatcgtgt cgcggcgcaa cagcaccgtt
     2521 cgccgcgtga tcggcttctg catggctagg ctaccgagca aggggtatac gataccgagc
     2581 gactcccctg gatcaagctc ctaagccatc ggattcgctc aaggagtcgg atctagtgtc
     2641 acggtatcca gccgcacgtt gccgtggagc catggcgagc ccatcggatc gccgtcgagg
     2701 cccgggacga cacggagctc tgcccaccag gatcccggca cgaatcgcac tttcacgtgc
     2761 tcgtcgcgcg aaaacgtccc ccacatgccg gtgccgtcac cgaggtagac cgccgtcccc
     2821 atgcgaacgg tgccttctcg ctcgacggcg ccggggaaag ccccctcgcc aaacatgccg
     2881 ccaccgtgat cgccggtgca catcaggccg ccgtagatac cgacgtcacc gggaatctgt
     2941 ttgacgaggc tgcgccgaac ccagccgcgc gcttcgacgc tgccgccata cgcgtacgca
     3001 gcaacgagga gccagtgctt cgtgcgcttc acttcttcga gcttgatgca gccgctgcac
     3061 gacggtgact cgaggaggcg cgctgaagcc ttcatgttcg gctgcttgta gagcgcgatc
     3121 ttcgaggctt caccgcgaag ctcccaacgg tgcacgacct cctcatcgtc gacgggtagc
     3181 gcgtcgtccg tggtggattc gatcacatcg agggagagcg cgtcgcaggg aacctcgaca
     3241 tctctgacga attgatcggc gggcttgtgc tcgccttcga ggacatcgtc ctcctcgagg
     3301 atcgccgacg cgacgacgac atcgccgcgc atgcacgcat cgatcacctg tgcgcctcgc
     3361 tcgagtgtga cgagaccgtc gggcgtcgtg atcttccgcg tcagccggag gcgcacgaga
     3421 tcgcgcgagg ccgtgaacgc gatcgcgccg cgaacatcga gctcgagtgg agcgccacag
     3481 cgcgcgggaa tgtgcgcgat cgccggcacc gcctgaaggc cgagctgaaa cggttcctca
     3541 tgcggcacag ccaccgtgat ctcttggagc gtgacggagc tgcccacgac cgcgcagcgc
     3601 gccggatctg cgtgcgcaga tgcgacgagc aacatcagca cgagcaccgg gcgcacgttc
     3661 gatagacgcc gccggctgca aaaatgtccc gaaaagatcg cgtttattac gccctcacgg
     3721 aatcgtcgca gagagccgca ggatctcgaa ggtcagctcg gctcccagtg cgaggacggc
     3781 ttctgcgagc ggccggtcac cttcgatggt gacgcgcacg taatcaaact gcggatcggc
     3841 gtaatgccgc atcgcctcga gcagaggaat cgcgaggttt ggtgagcgcg tgcaaaacgt
     3901 cgcggcgccc gggaagcctg ggctgaaggc ggcgaagccg aggagcgcgt ccgcatcatc
     3961 gcgaagcgtg acgagccgaa agctgccgcg cgcgcgaaag ctcgcgacgc gatccgcggg
     4021 cacgttgaag aggcgctcga tcgtcgcgtc gtcggcgctg gtgtcggcca agcggtggcg
     4081 ggcagcgacg cgtggaacgt ttgcccatgg cagcttcatc gcgaacgact cgagctcgat
     4141 gcgaagtccg acgcgctcgt agagcttcag cgcgggcgcg ttgtcgcgct tgacgttgag
     4201 ataccagcgc gtgcagcccg ccgctttcgc ctcgtcgcgc gcatggacga gcagctgctc
     4261 gccaaggcgg cgtccgcggt gtgcgggatc gacggcgagg tgcatgacat gcgcgagcgg
     4321 gccgtagccg cgccacgaca tgtagccgat cgcctggtct tcctcgtcgg tgacgaccgc
     4381 gatgcgctgc atgttggtgg cgacccactc ggcgcgctcc ggcaccggat cgggaacgcc
     4441 gagctctgcg tggaggcgaa cacacgcgtc gtaatcgtcg gggcgtgcgg cgcggatcac
     4501 tccgatatcg tactgcgtgc gttatcgcta cgcgcgggca tccgcgcacg ttgctcgtgg
     4561 ctaagtcagg cgcggctccg tcggcgcgtg gcacggccga tgctcatttc tttgggcatt
     4621 atgcgttcat tcaaggctct attgtttgca gccgttgttg ctggttgtgc gggttcgggg
     4681 gcggttcgct atagcgcgac cgcagaggtc tcgacgcccg agatggtcga ggtcgagccc
     4741 ggcgtgatgg tcgtcgccga ctacaacgag ccggtgttct acagcgaagg tgtgtactgg
     4801 cgttacgaga gcggcacttg gtaccgctcg ccgtactaca accgatcgtg ggttcgcatc
     4861 tcgacgccgc cgctcgcggt tcgtcgcatc gagcggccgg agatttacgt ccattatcgc
     4921 gcgggtgctc gcgaccgtcg tgaagcgcgc gcgtatcagc cgcagcagaa ccccgaggtt
     4981 cgcgaccacc gcacgtaccc gcagcccgcg cctgcaccga tgcccgcgcc ggcgcctgca
     5041 ccagacgttc gagatcaccg cacgtacacg ccagcgccga cgccgcttcc gccgtcgccg
     5101 ccggatgtcc gtgatcaccg tgacgaccat cgcgaggatc atcgcgacga tcgccgggac
     5161 gatcatcgcg acgaccgcca agaccgtcgt gaagagaagc gcgacgatcg acaagaccgt
     5221 cgcgaagaga agctcgagcg caaagaagag caccgcgagg acaaggcgga gcgccgtgaa
     5281 gacaagcaag agcgccgcga ggacaaggcg gagcgccgcg aggacaagca cgagcaccgc
     5341 gacgacaagc acgacgacca caacaagcac ggccaccact gaaagcagcc cgccacggag
     5401 ctgggtgcct gcgggtccgt cattcggaca gcgttcggca cccgctcttt gagttgcgcg
     5461 gagatgggcg acactcgtga agggtgctcg cccttgccgt cgtcgtcagg agacttagct
     5521 ttcctgtcat ggtggacacg aaatcgatct gggacgacat caagcggatg gcgcaggagc
     5581 tcgaggtgca aatgcatctg gccgagatgg aggcgcgcga gcggtggaca gctctgaagc
     5641 cgcgcatcgg cgagctccaa aaagacatga gcgacaccgg caagaaggtg aacgaggtcg
     5701 tcgagaagga gctgtcgtcg gtgggcgcgg ctctgcgcaa gctgcgcgac gacatcgtca
     5761 agcgctagcg attcggcgca tcggcgagga gtgagctacc ctagcggttg ctccattgcg
     5821 tctgctcctc atcacgccgc cgatgacgca gctgaacacg ccgtatccag cgacggcgta
     5881 tctcgccggc ttcctgcacc aacacgcgag tcggctcgac ctcgaggtgc atcagtccga
     5941 tgccgcgctc gagctgttcc tgcgcgtgtt ctcgcgcaag acgctgcagg agatcctcga
     6001 cgagctgacc gcgcgtgccg cggaggtcga cgaggacgac cgtgccgaga tgccgccgtc
     6061 gatcgcgagc ttcttgcagc atggcccgcg gtacgtcgcg acggtcgacg ccgtcgtgcg
     6121 gtttctgcag ggcagggacc cgggcctggc gctgcgcatc gtcacgcgcg agctgctgcc
     6181 cgaaggcccg cggtttgccg cgatcgcgca gggcccgggc gcgtcggaag ccgatccgct
     6241 ggcatgggcg ttcggcggcc tcggcaccgc ggatcgcgcg aagcatctcg cgagcctcta
     6301 catcgatgac ctcgccgaca tgattcgcga cggcgtcgat ccgcagttcg agctgtctcg
     6361 gtatggcgag agattggcgg cgagtgcggc gtcgttcgat ccgctgcatg aatcgctcga
     6421 aggtgatccg tcgctgatcg acgagctgct cgacgagatc gcgcgcgagc tgtacgagcg
     6481 gtacaagccg gatgtcgtcg gcctgacggc accatttccc ggcaacgtgt acggcgcgtt
     6541 tcgcatcgcg cggtcgatca aagcgagcgc accacgcacg acgctggtcc tcggcggcgg
     6601 ctacgtcaat accgagctcc gcgagctgtc cgagccgcgc gtgttcgact atttcgactt
     6661 catcaccgtc gacgatggtg aggcgccgac gctcgcgctc gtcgagcacc tgcgcgatac
     6721 gggcaagccg ctcctgcgca cgttcgtccg ccgcgatggc aaggtcacgt ttcagaccga
     6781 cgacgcgttg cacgacatcc cactgcgcga tgcaggcacg ccgacgtatc gcgaccttcc
     6841 actcgatcgc tacctgtcgc tgttcgagat gctcaacccc atgcatcggc tgtggtcgga
     6901 cgggcgctgg aacaagctga cgatcgcgca cggctgctac tggaagcagt gcacgttctg
     6961 cgacatcacg ctcgactaca tcgcgcgcta cgaccgcgca ccggcggact tgctcgtcga
     7021 tcgcatcgaa cagctcgtcg ccgagaccgg gcagacgggg tttcacttcg tcgacgaggc
     7081 cgcgccgcca gccgctcttc gcgcgctcgc cgagacgatc atcgcgcgca agctcacgat
     7141 cacgtggtgg ggcaacgtcc ggttcgagaa agcgttcacg cccgagctgt gcaagctgct
     7201 cgcagaatca ggctgcgtcg cgatcagcgg cgggctcgag gtggcatccg atcgcctgct
     7261 cgagctcatg cgcaagggcg tcaccgtcga gcaagtcgcg cgcgtgaccc gtgcgttcac
     7321 ggacgcggga atcatggtgc acgcatatct catgttcggt tttccgacgg agaccgcgca
     7381 agaaacgctc gattcgctcg agcgcgtgcg tcagctgttc gctgaagggt gcatccagtc
     7441 ggcgttctgg catcggtttg ccgcgacggc gcacagcccg atcggccaga agccggagct
     7501 ctacggcatc aagccgcgca agccgccgac gatcacgttc gcgaagaatg acgtcgagtt
     7561 cgacgacccg acgggcaccg accacgactt cttcgcgccg ggccttcgca aggcaattta
     7621 caattacatg cacggcctcg gtctcgacgc agaccctcgc acgtggttcg gcaagcgtac
     7681 gaagctcgcg aaatctaccg tgccgcctga tctcgttcga cgagcgcttc gatcagcaat
     7741 ttgagtgtga cgcgctgacc gcggccgccg cgtcgacgcg cgagaaggcc acgttctgtg
     7801 tgccttgaat tcgctttctc gcacattcga cgtcacttgc agcgcgcacg gatgttgctc
     7861 gatgcggcgg taacaacgga gggaagccat gaagacgaaa tttgcgctta ttacctgcgt
     7921 gctcggcgca gcactcgcgg gatgttcgga tcaccaggac acgcgtcgtg acacgacgcc
     7981 tgccgcgacg acgcccacga cgtcgcccga tatcaacgcc accaatggcg gcggtgcagc
     8041 agcccgctcc ggcgacatgc ggacgcgtgg caccggcatg ggcggtacgt ccggcacagg
     8101 ccagtacgac gacccctcgg gcgtcaacgg caccggcggc tcgatggacg gcaccggcgg
     8161 ttcgatgaac ggcaccggcg gttcgatgaa cggtaccggc ggttcgacgg gtaccagcgg
     8221 ttcgatgaac ggcaccggct ccacgaccgg cacgggcggt tcctcgggaa attccacaac
     8281 ggggagctcg ggtgccgcgg gttcctcggg caccagcgga tcgtccggaa ccggcaccga
     8341 catcaatggc tcatctaacg gcggttcgac ggacagctcg acgagcggct cgacggacag
     8401 ctcgacgagc ggttcgacgg gcacgtcgac gaccaagccg acgaccacga agaagaagaa
     8461 gccgacgtcg agctcatcgg gcaccggcac gagcggctca tcgggcaccg gcacgagcgg
     8521 ctcgtcgggc acctcgggct cgtcgggctc gacgccgaac gatacgtcga gcggcggaac
     8581 caaccgctag gaggctccga tgttcgtccc ggtcctgatg atcttgctat tgctcatcgc
     8641 aaccgccgtc ggcgtttcga tcaatgccaa gaagaagatc gacggcgttc gcgacgagac
     8701 atagccagtc gtatggatgt tggcgctcag cgcaggtcga ggtgcaggaa ctggttgaac
     8761 tcggtggcta cgtagtcacc gaacgccggt ccgtcgacga agccgtgctt gcgatacatc
     8821 gcgagtgcag cttcgaaggc gggcccactg ccggtttcca gacacacgcg tgtgtagccg
     8881 cgcgaccgag cgagcgagag gagatgcttc agcatgaacg cgcccgcgcc tcggcggagg
     8941 tgtcgggccg tcgtgcgcat cgacttgatc tcgccggtct gcgcgtcgac gtgcttgagc
     9001 gccccgcagc cgagtagatc gtcgccgtcc cagacggtga aaaatgcgat cgacgggtgt
     9061 tgcaggcccg agagatcgag cgcgtagacg ctgcccggcg gcgagctcgc gtgcatgccc
     9121 gccagatgct cgcgcaggag gtcgacgacg cgctcgtcgg tcaggtcacc gggtttgatc
     9181 tgcatgacaa cgtcagcggc ggcgctgatg acgcgggttg tgaccgggct tgcgtggttg
     9241 ctggtgctgt tgctgcggcc ggccgtgctg ctggtgcggc cgcgatggac cgggcgactg
     9301 cggttgttgc gcgggcttct tcggaaacag cgcggcgacc tcttggtctg agagattgcg
     9361 ccagcgaccg agcggcagct ggccgagctt gatgtgcatg atgcgaacgc gctgcagcgc
     9421 ttcgacggtg taaccgaacg cttcggtcat gcggcgaatc tgccggttga gaccttgcgt
     9481 gagcacgatg cggaagactt ttgcgccgag cttctcgacc ttgcacggct tcgtcgtcgc
     9541 atcggagagc cgcacgccgc tcgacatctt cgtcacgaag tcggcatcga acggtttgtc
     9601 gacggcgacg acgtactcct tctcgtggtt gtgctcggcg cgcagcactt cgttgacgat
     9661 gtctccatcg ttcgtcagta gaatcagccc ctcggaatct ttgtcgagac ggccgatcgg
     9721 aaacacgcgc tctggatggt cgacgaagtc gacgatgttg ccggcgacat ggcgctccgt
     9781 cgtgcacgtg atgccgactg gcttgttcag cgcgatgtag accggcttca ccttcttgcg
     9841 cgcggtgcca acgcgatcgc cgtcgagccg gacgtcatcg ccttcattga cttgcgtgcc
     9901 gagcgccgcc ggcgcgccgt ttaccgtgac gcgaccttcg gagatgaacg tgtcagcctc
     9961 gcgtcgcgag cacgcaccgc tctcgctcag atacttgttc aaccgcacgc ctcggcagag
    10021 tagctcgtct tacagtgatc ggggatggcg aagacggact acgcggcgca cgacgctagg
    10081 tatcggcagt tgcgcgagga aggcgcaccc ggttgggaca ccgccgatgg ctatcgcgaa
    10141 cgcgaagcgg aatacgcgtg ggcgatcgac gcgatcgacg cgatcggcag gccgggcaac
    10201 cgcctcctcg agctcggctg cggtgcgggc aacgcgacac catggttcgt cgagcgcggc
    10261 ttcgaggtca ccggcatcga catctcgccc gctgcgatcg cctgggcgaa cgaacgcggc
    10321 atcgcgggtg cacgattcgt cgtcggcgac ctcgtggcca gcattccagg tcagtacgac
    10381 atcgccgttg acggccactg cctgcactgc atcatcggcg acgatcgcgc gcgcttcctc
    10441 gcgaacgtgc gcaacgcgct cgcgccaggc ggaagcttct tcgtcgcgac gatgtgcggc
    10501 gagatcacgc taccgaagct gcgcgcatcg tttgatccag cgacacgctg tcaggtcgtc
    10561 gacggaatcg cgtatcggta catcggcgat gcctccgaga tcctcgacga ggttcgcgca
    10621 gcgggcttcg aggtcgtcgc gtcgacaata aatgcccgca aggacgataa cgatcaggac
    10681 aatctctggg cgatcgcgcg caagcggtag atagacctag tcgagcgcac gggacagcca
    10741 ttcacgaaac cgcggtgagt ggacgcgctg gagtcccttc gcggcatgcg ccttcgcata
    10801 gcgcagctgc tgcagcggct cgcgcacgat cgcaccgcca agcaggtaat cgtggaatgc
    10861 gtcgaagtcc tcggccggcg cgccgtcatc aacgacgcca ccacgcttcg cgatcgctgc
    10921 aagaaacgtc gcgtagaaca cgtgctccgc gatgcggatc agctcgaacc gcgcgcgctg
    10981 ccctggtgac ggcatcgaac cgtaatagag cgtcagataa cgctcgagcg tcgcttcatc
    11041 gcgcacgaag aagttcgccg ggatgacgag gtcgaagttt cgatcgtcga ggaacgccgc
    11101 ctcccaatcg agcagcagca agcgctcgcc gtcgaacgcg atgttctcgg gcttgagatc
    11161 gtgatgcgac gacacgtggt cgagcttgcg cggatacacc gccgcgacat ccgcaaaccg
    11221 atcgaacaag tcaccgacgt cgaaggcgtc gaactgcccg acgaatttct cgatcaccgt
    11281 gaattgatcg atgctcgggt gaaacgaggc cagcgcatgc aaccggcgca gtgtcggcgc
    11341 gaggtgcgcg accatgtcgg gcggaaacgg ctttcgttcg acgaaatcgg taatgacgat
    11401 cttgtcggtc gtgcttgcga agtacacgcg cggcgcgatg ccggcgtcgg cagcatgctg
    11461 catgtagatg aagtggcgcg tcggatcgga cccgagcgta ttcaggtgaa tgcgcagcac
    11521 gtacgagcgc ccgcgaacct ccagccgata cacgagcgac tggctcatgc cctcggtgag
    11581 cggctcgatc gcggcgggtt cggcgactcc aaaggcggcg tggagcgcat gcgtcacggc
    11641 ttcgcgatgt ggttctggaa tcatcgccac gtgatcgcgg attgcggccg catttcctag
    11701 cttgttagct cgcgcggttt ttgcgatata tgtagcggga cgcaggcgtg gcgcccggcc
    11761 ctcagtgctg gcaggcgccg tagctgaagt cagacgtcag gtgctgaccg ccgccgtcga
    11821 gggtccagcg cgtcaggcgt ccgtacgagt cgtacgaaaa gttttcggtg atgtcttctg
    11881 ccgagccgta gagatcctcg ctgtagcggc tgatgtcctt gcgcgagttg tacgagtagt
    11941 cgtcggtgat gtccgccatc gagccgtacg catcgtgcga gaaccgcgac agcttgttgt
    12001 tcgagttgta cgagaagtcg tccgtgaagt cgttcatcga gccgtaggcg tcgtggctcc
    12061 aacgcgacag cttctgcgac gagttgtacg agaagtcgtc ggtcgtgtcg ttcatcgagc
    12121 cgtagccgtc gtacgagacg cgcgtcaggt tgttggcgct gttgtacgag aagtcgacgg
    12181 tgaagtcgtt ctcgctgccc gtctcatcgt gcgagaagcg cgtgatcttg ccggcgctgt
    12241 tcgtcgatgt gctgccgctg acgctgaagt gcgtgagcgc gtcgcgcttc gcaatcgctt
    12301 cgacatcgcg attgacgaac accgagtccg ggagatccgg cggcgtgtag ttcgtgcagc
    12361 tcgtcgactc ggagccagag ccggatcccg aaccggagcc ttcgcccgag cccgagcccg
    12421 tgtcgtcacc gacatcgttc atatcgctcg tcccgcaagc cgcaaccaag accaacacga
    12481 gggctctacg catgggcgcc ctcgtagcag cggcgcgccg cgtgacgcaa cgccaaaaat
    12541 ggccgcggaa cgcgccagtg gcccggtttt gcgatctagc tgccgaaact aaccgctagt
    12601 tgagatcgga cggcaagacg ggatcgagca ggtgcgcccg gacgcgcgtg cattcggcga
    12661 gcgcctgcgc gagctgagaa tcgcgctttt tcagctcgcc gatggcagtc gagagcgagc
    12721 cggacacgcg tgagacctcg ggcaagattc cctcgatcag acgacggttc gtccacatga
    12781 cgtcgcgcaa gatgaaccgg ttcgtctccg gcagcggctc gagacgcaga tcaaaatcga
    12841 tccccggcgg cagtgtcgtc tgcagcgtgt gacacgcttg ctcgaagcgg cgcaggcctt
    12901 gctgagctgc cgcgagcgcg ccgcacaaac ccgtgtagct ctcccgcgtc caacccatca
    12961 tcgcgtggac gagtccttcg cgctcgactt catcttgagg ccgcggctcg agctcgcgcg
    13021 cgtaattgac gaccgcgatg aacgacttct ggacgtcgac gccgatcgtc atggcctcgc
    13081 gcacttcacg ccggctcgac gtcagctcgc tctcggcagc gacgagcgca tcgagctctt
    13141 cgttcagctc gccctgctcg cgcacttctg cctcgacctc ggcgagcacc tgcgcgtagc
    13201 gagcgtctgc atcgcggacg gcgttctcgc gcgtcgtcag ctgcgcaata tcggcatcga
    13261 tcgaacgcag ctcgtcggtg agctcgtcac agagcagctg cgccgccgcg agctcctgct
    13321 gctcctttga cagatctgcg tgatctgcca tcacgcgccg gaagaacgtg ccgatgccct
    13381 tcgtgagcgc atcgacgtct gcttgctcgt agtcgcgtgc ctgctcggcg atcgcgagtt
    13441 gcgcagcgag ccggtcgcgc tcctctcggg tttccgcgag acgccgccgt acccatgcaa
    13501 gctcgcgctt gtcggcccgc gctttggaga ggcgatcgtt ttgactcacg gcgagagtct
    13561 atatcgctcc cgcacctcgt cgcttcggac gtgtgacttc acgagggccc agtcgccgag
    13621 cgagtcgagc gcctcactcg tcgcggctac aagcgtgcgc atctcgatgt cgttcgtcac
    13681 gacgcgggac acatggcgac cgaggtcgag ccacgtcttg cgttcggcgt cgggattcct
    13741 gctggcctcc gccgtcagac gcgaacgcac gcgcaccgca tggagccacg ctcgctcgac
    13801 cacgaacagc gcccccaacg cctcgatctc ggtccgcggc gtttgatcga acgcgcgctg
    13861 cgcgagccgc ccgatctccg cgatgtcgtc tggggacgca ccccacgcgt ggagctcgaa
    13921 gacgagcgtt gcggcacgcg catggatccg cgccgacgca tagagggagg cacccaccgt
    13981 cggtgcaata cccgcggtaa agccgtagac gtcgcaaagg taacggacgt acccctcggc
    14041 ggacgcgctg gacatcaggg cgtcgagctg ctggtccacc atctcggtgc ggtcgcgaac
    14101 gctgcgcagt aaccgaacga gaggagtgga gatcatcgtg cgcgcatctg tgcaagaacc
    14161 gtagcgaggc gaacctcgcg ttagccgtgg ctgcggacag cgccgcaccc gaagagcgcg
    14221 tgcgactgcg cggcagtgcg atcctggcgc tcgatccgtc cggtgacgtt cgctgttacg
    14281 ctagcgcgcg agtagctcct tgattccttg gaaggttctt gcaacggtcg acacgtccga
    14341 ggggcccctc cagctccgcc agcgcggcgc gcgcgagttc ttgatcacga tcggcggccg
    14401 ggtcctgatg accagcagcg agcggacctc cgagctcgcg gtggccacgc tcgcttgcaa
    14461 ggagcttgcg ggacacaagg cgccgcgcat cctgatcggc ggcctcggca tggcctacac
    14521 ggtgcgcgct gcgctcgacg cacttccgaa gcgtgcgcag gtgatggtcg cggagctgac
    14581 gcccgaggtc gagacatggt gccgcggccc gctcgcgccg ctcaccaacg gtgccgccaa
    14641 tgatccgcgc gtccggatcg tcattcgcga tgtcgcgcgc gtgatcgaag aagctccacc
    14701 gggccgcttc gacgcgatcg tgctcgacct ctacgagggt ccgcacgcag catcgaggcg
    14761 cgagaacgat ccgttctacg gccgcgttgc actcgagcga agctacgccg cgctttcgcc
    14821 cggaggcgtg cttgcgatct ggtccgaaga atccaacacg gtgttcaagc gccgcatgat
    14881 cgacgccggc ttcgacacca cgctgcacca tccaggcgga agccggtcgt acgtcgtcta
    14941 cctcggtcgc cgagaatcac cacgcgagcg acgataatgc ggatcaagcg gagcgccgat
    15001 tgcggcaact caccgaagaa caagttcgcc gaggatgtgg tggtggcgct atggaaagga
    15061 aaggccgccg agcttgcacg actcgttgca gaagattgcc gctgcgacgt tgctggcgat
    15121 gcgaccgttg tcgcgccgcg agccctcgtc ggtcaggcct ttgggacaca gcaattcgac
    15181 gacgtctctc tgaggacggt catcacgcac ggtcgcgtcg gcgcggtcga aggcgttgtc
    15241 gacatctccg gcaccgcttg gccgttttgc atcgtgatgc gatttacgag tgcgaaagca
    15301 gagagggtcg gagagcttcg cctctacgcg cccatgcgac acaagtgaat ggcgcccgct
    15361 aggcgtgccc ggtaggcgcc acctgtcgca gcacagttcg tagccggtcc atatctaccg
    15421 gtttgacgag atgcgcgttg aagcccgact cctgcgccgc gcggcgatcc gtctcctgac
    15481 cgtaaccggt gaccgcgaca agacgcagcc ccgcgagagc cggctgcccg cgaagcaagc
    15541 gtgcgagctc gtatccatcc atcgctggca gtccgatgtc gaggaccgcg atctccggct
    15601 tgaaatcgtc cgcaacaacg agcgcagacg gaccgtcatg cgcggtgcgc gtcgtgtagc
    15661 ccatcatctc gagactctcg gccaggagct cggcagcgtc gacgttgtca tccacgacga
    15721 gaacgcgcat ccgtatcgac tgcgcctcgg cgtcgcgatt cgtcgagctc gcgttcgtaa
    15781 ccgccgcgct gacctggttt accgcaggca gcgtgacaac gaactcgctg ccgcacccac
    15841 gaccggcgct cgacgcctcc acgagtcctc cgtgcatcgc cacgaggttc tttacgatcg
    15901 ataggccgag gccaaggccg ccgttcgcgc gatcgagcgc ctgacgctcc tgcgtgaaga
    15961 gctcgaacac acgcggcagc atctctgccg aaattccgat gcccgtgtct cggatccgga
    16021 tcacgatccg agcgtcctgg cgagccgccg tgacggtgat gacaccgccg ggctccgtgt
    16081 acttcgcggc gttcgtcagg aggttcgaga acacctgggc gagacgagtc ggatcaccgt
    16141 taaccacgag cccacgagag ggcacatcga gcgtcaactg ctgtttccgc tgctcgagca
    16201 tcgagctggc catttcgagc gccttggcga tgacttcacc cacttcgatc ggctgcttct
    16261 tgagctcgac cttcccggtg gtgattcgcg aaacatcgag cagatcatcg acgagacgaa
    16321 ccaggtgatt cgcctggcgc tcgatcacgc tccgctcttt ttccagtgag gtgtccccgc
    16381 ggagctgcat gaggtgcaca gcggtcaaga tcggcgcgag ggggtttctc agctcgtggc
    16441 cgaggatcgc catgaactcg tctttggcgc ggttcgctga ttccgccagg cgccgcgctc
    16501 tcgccagctc cgtgacctcg aacacgacga cgacgatgcc gtccaccgcg cctgcctcgt
    16561 cgcggagcgg ctggtagacc gtgtcgaaga accgccgctc cgcaacgccg tcggtttggc
    16621 gaacgagcac gatctcgcgc gaccgcacgg actgcggctc gccgctcgta tacaccgcgt
    16681 cgaggagctc gtatgagcct tgtccggcga gatccgggaa tgcctcgcga atcgtcttac
    16741 cgaggacact tcggtcgtgt acgagcgcgg cgtagctcgc attcacgaac tcgaagcgat
    16801 gctccggccc acgcaggatc gcgattccga cgggtgtatt cgcaaagacg ttcgcgaggc
    16861 ggcggttgtg cagctccgtg accgcgcgca cctgcatttt cgcgagctgc gtttccactc
    16921 ggaccaccag ctcgcgcgca gagaatggct tgaccacata gtcgtcaaag cccgcgcgca
    16981 atccctcgac gcgcgcctct tcgccggcac gcgcggacaa cgcgatcacg gggaggtccc
    17041 gtgtcgcctg atcggcgcgg agtgcgtgaa gcaagctgac gccatccatg ttcggcatca
    17101 tgagatcggt gatcacgaga tccggtcgcc ggcgtcgcac ggcggccaga gcgagcgcac
    17161 catcagcgac cgcgtcgact gaccagtgct gcccgagaag tcgggcgagg tattcgcgca
    17221 tgtccgcgtt gtcgtccgcg atcaggacat gcgtcgtcga attggcagcc accggtctgg
    17281 gcgcggatgc acttggatcg acccagcgca gtgcttcgac cacaaacggc ttggccgtgc
    17341 tctccatgtc gtgcgcacgg acgttcgctt tgatgtgcga tgccggaaga tgcgcgcttc
    17401 cgcggggcag ggtgatccgg aacgttgtgc ccttgtcggg aacgctgctc gcaacgatgg
    17461 tgccgccgtg cagctgcacg aggtcgtgca cgagcgcgag cccgatgccg gatccctcgt
    17521 gcgtgcgtgc cttggttccc tcgatccgat ggaaccgttc gaacagcctc ggcatctcgt
    17581 gctccgggat gcccacgccg ctgtcttcga cctcgacctc gacgtggtcg cccacgatgc
    17641 gctgcgagat ccggatcgag ccttcgaacg tgaacttcag cgcattcgag agaagattga
    17701 gaacaatctt ctcccacatc tcgcggtcga caaagatcgg ctcctgcaac gaaggacagt
    17761 cgacttcgaa gcggatgccg ccgcgctcga tcgccgaccg aaacgcgctc gcgagatcgg
    17821 aggtcagtgc cgccagatcg acgggctcat acgctgcggt cgctcgccct gcttgaacgc
    17881 gagcgaagtc gaggagcgag ttgacgagct tgagcaagcg caaggtgttg cggtgaacag
    17941 cttgtagatc cgcgccaccc agcgaattag ccggcgactc cagcgcgtct tgctgcgggc
    18001 cgagcatgag tgtcaacggc gtgcggaact cgtggctgat gttcgagaag aacgcagtct
    18061 tggcgcggtc gagctcggcg agctcgtcgg ctcgtcgacg ctccgcttcg agcgttagag
    18121 cattggcgac gacgcgagtc accgtggctg ccgctcgctc ggcgaactct tcgtactcgc
    18181 gatcgacggc gcgacgggga ctcgcgccga tgatcagaaa tccccagctg tcgggtgcag
    18241 ccgtcgaacg aatcggagcg accatcgcgc tgcgcgtcgg ttccggccac gccccacctg
    18301 agagcggcac cggcaactcg tcgaggcggt ccacgatctc gaccttgccc gaccgacgaa
    18361 cgtcgccaaa tcgccaacgc gactcggcaa cgtgctcggc cgcgatgtac gcgggcgcga
    18421 tcgcttcggc gtcgtcgagc ccagtccacc ccacccgccg cagaccgccg ctctcgtccg
    18481 ccagatagat gacggagaac gtagcgtcga agcgtgcggc gccgagagtc ttgacgacgc
    18541 gctccgccac atcatcgacc gagcggtcgc tgatcgcgtg ctctgcgagg tttcgcaaaa
    18601 cgctcgcacg gcgttcgctg atcacgcgga aggtcgtctc gttgacggcg ttgaagactc
    18661 cttcgatccg accgtcgtcg ccgcgaacgg gcgtgaacgt gaagttgaag tagcactcct
    18721 ccgtgtaacc gtgccggttc atcaagagga gcccgtcctc ttggtaggac gactcaccgg
    18781 ttgcgagcac ggtggagaac aacggaccaa tcgcgtccca gatctcgggc cacacgaccc
    18841 gcgccgcatt gccgagagcc tgcggatgct tcgttccgag gatcgggctc cacgcgtcgt
    18901 tgtagatgag cgtcaggtcg tcgccccaat agagcgcgat cggaaatcgc gagccgaggc
    18961 agatgctcag cgccgaacga aagctcggcg accacgtgga tggccgccca accgcgacgt
    19021 tcgaccaatc gagctcgcgc atccggcgac ccatctcgcc cccactcgga acaatgcggg
    19081 cgatgagctc tccgtcatgc tgaggtacgt cgagcatcgc aggcagtctg gcgcaagtct
    19141 gcgagcgccg gtaggcgcgg tcgcgacaag ccgatgctgt gcgcctacgt cacgaaatag
    19201 cggggacgcg gtgcgcgaat cactgtcgcg acacgcgcac cgaaagagct cgccctacca
    19261 gcgatctccg ccgccgccgc ggccacggcc gccgcgacca ccacggccac cgccgcgatc
    19321 gtcgccacca ccgccgccac caccccagtc gccaccgccg ccaccaccac cgccgccacc
    19381 accacggtag ccaccgccgc cgccgccgcc gccgccgcgc tgctggcgct gctcggcttc
    19441 gttcacacga agcggccgtc cgtcgagggt ctggccgtcc atgctttgca tggccttcgc
    19501 cgcttcatcc ggcgtgctca tggtgacgaa cgcgaagccg cgcgcgcgg
//
LOCUS       DATLBZ010000078        13424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3014580, whole genome shotgun sequence.
ACCESSION   DATLBZ010000078 DATLBZ010000000
VERSION     DATLBZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13424
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014580"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VMZ53_04220"
     CDS             complement(<1..353)
                     /locus_tag="VMZ53_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27683.1"
                     /translation="MRIALSLLALLAACDYGNGQMSDDDSEPDASMPDSPTGCAPGKA
                     GAPCVLALYDQAAAGCDAKAVTDLQTELDARASLGPLWAEGRALFRTQAPTHVAGTFN
                     DWSTSALETKPFCGSS"
     gene            501..689
                     /locus_tag="VMZ53_04225"
     CDS             501..689
                     /locus_tag="VMZ53_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27684.1"
                     /translation="MSVLCVGGLVYALIVERDTPAELRSTTGPVSSGGNSGFALGVLV
                     GVGAGIVIGSLIALRRRS"
     gene            complement(686..1261)
                     /locus_tag="VMZ53_04230"
     CDS             complement(686..1261)
                     /locus_tag="VMZ53_04230"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HUS27685.1"
                     /translation="MIALADIGPATPVLHTERLALRELRIEDAHSIAMRAGDKNVAQY
                     LIAVPSPYPVALAARWVAARIAWWPQARGLTLAITKHGAPNQLIGTVSLRRNIRDRRA
                     ELGYWLGMDAWGQGYATEASDALIDLGFGQLGLQRIYAQVLDGNDASCRVLEKLGMLS
                     EGIRRAHVRKGKRLCDVHMFGMLVDEWRDRR"
     gene            complement(1269..1739)
                     /locus_tag="VMZ53_04235"
     CDS             complement(1269..1739)
                     /locus_tag="VMZ53_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27686.1"
                     /translation="MKRLLLVVAMAACGGDPVDVEGTYSVGLTNRENGCNFQNWTVGE
                     MTSGVTVVITQNDVNAIAEVQGIAGGYLNFAFGSNTFSGSVDGDELDLSLSGTRPQTS
                     GNCTYTYDGRIQATLTGNALNGTLSYASNGNDNTDCAAIVCTSVQEFSGSRPPK"
     gene            1790..4534
                     /locus_tag="VMZ53_04240"
     CDS             1790..4534
                     /locus_tag="VMZ53_04240"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS27687.1"
                     /translation="MPGSSTRAYHHAAAALAITLGAGPASAAVHDVETHRLDNGLTLH
                     VAGGHPAPVVAVQAWVGVGSADEQPPQAGVAHVVEHMLFKGSSSYGVGELTRAIEGSG
                     GEINAWTSFDHTVYHAVLGRDHLDGALDAIGDTLMAPRVDPDELARERRVILEEIRQG
                     TDDPARSVAQSLFATAYVAHPYRRPVIGTAESVQRLGERDLVEFFRSYYVADNVTLVV
                     AGDVDPARVIRSVERRFRGMPSGRPTRRIPHEPAQTAPRAVAAHRDVSEAYLAVGFHV
                     PAARHPDVAALDVAAMLLGESESARLPRTLRDRDQLVTGAYAHVHALRDPGLFVLSAT
                     ARARDAEKSVGALVDQALGLVNDLSQDELDKARIAAEAGLVRQLETAQGRARVLGWNA
                     SMNGDPRFAHVYLDRIRAVRRHDVAAAVHRYFRSENATVAAILPKPTRASTKTHATFA
                     RGAEKRVRSALRIATAPTTSAEKRVVLQNGMVLLVRRDASVPIVAMRAVWRGGQRIED
                     ADHAGASTLLARLITRGCGQLDANQVADRVDRLGGSLGGVSGRNSFGLASEWLAKSWR
                     NGFDLIADCLLEPQLAAEELTREKRALYDDQTAQQDNPTQVAFRLFSETLYGSHPYAR
                     DVLGTADAIGDLDRTALVAFYNDQYPISALTLSIVGDVDVDDVIARVTKRFGDLSSKK
                     PPSPSVALPSFDNRPAAKREVYRFLDRAQAHLVIGYPGATVDAKDRFALEVLIAILGG
                     QSGRLFSELRDKQALVYRVSAHSVEGVDPGFVAVYLSCAPANLEAAITSVRGELTTVT
                     KDGAITADELERAKSYLVGSHQIAMQRRSAVANAIAYHEAYGLGWKTWASYDDAIKAV
                     TLDDVAAAARTYLRDDRAITATVRPPLETPAAAKKSKLKPQPPKLKAPEPAKKPRKKR
                     "
     gene            4596..5276
                     /locus_tag="VMZ53_04245"
     CDS             4596..5276
                     /locus_tag="VMZ53_04245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HUS27688.1"
                     /translation="MSRQRSNTAGWAGHGDLEASLVLIFPVLLAYEVGVLFGGRVNGA
                     DVITRLLYTLLGSKTLYLLVHAVVALWFLLWARARGKWGTLRMEIVAPVVLEAAIYAL
                     TLGAVVNLVLEKLLGLGVGRESIISALGAGVHEEIVFRLGLFAGLTSLLESRQQRFAM
                     PVALIVSSIAFAAAHHLGAHGEPFTMHAFAFRALAGAAFGAIFWYRSLAHAVYAHVLY
                     DLVVVATS"
     gene            complement(5277..6212)
                     /locus_tag="VMZ53_04250"
     CDS             complement(5277..6212)
                     /locus_tag="VMZ53_04250"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HUS27689.1"
                     /translation="MGIRDLLTKDSYTAGHFELIIDGAPTTAFLKSIDGGWAKHSVID
                     EAIGPNNMRIKHASLLEIDPVTFDFGLSGANSVLKWIQESWNKNPTRRSGQITHGDFN
                     NTSKLEHHFSDALILETTFPALDGASKDAAYIKVKFQPEGVMTKKGDGKSLRGIDKGK
                     QKMWLCSGFRLNIDGIDEMKYCNKIESFTIKQGVKKMQTGAQRLAEIIPTKIEFPNIS
                     GTMSMAYADKLLEWHNDYVLKGKADPGAQRTGSLEFLSPDKSRTLFRINLSEVGLIGT
                     NVVASTANQDQIKRAKFDLYVGKMELDGGGSLGFE"
     gene            6360..8057
                     /locus_tag="VMZ53_04255"
     CDS             6360..8057
                     /locus_tag="VMZ53_04255"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HUS27690.1"
                     /translation="MRVVALILLAFGLLVGIGGSADAEKVKTNQSTKVYARPGEQAKV
                     IVTVKSGQNMTLLAKDGRWLKVRVNGRTGYVPRSKVDMADDDEIARNTRRRPFVDGRG
                     RRRGFGGDAPDDRVAADATGDGADSGDDEEEDEEEDEPAKKPVKTAAKSNAKKGGDED
                     EEEEEDEEDEKPVKKAPVKVAVKATEKKTPAKKGGDEDEEDEEDEEPAKTAAKAGGDD
                     EEEDEETPTKGGDEEEKADTRPVARVGKKVAVYEKPNLDSNEAFVVRPSDLLYPSDEK
                     NGWTFVENSEGDSGWIESTELSMEGGGGGGGMKKTIDVRARFGLMFLQQGVRTQGSTN
                     LNVPDNYNIGTSSIAMAVGAGILIPKGKKLLLGGEFTYDYAKTLLGGVFYDADGKGGS
                     PGVNIGVTIHNINARGMVGIDLKKKSGMAVFGRLGYRYQGFLITDVASMTANPAKLPS
                     EVVKAPTIGAALAIPKLSEKLGLRLSLDAIAFGASVKQTVGLEDGSAPSVKAVDVGAG
                     LTYKFKKAFDIQAAYDLQYMGIDFGPPLMSSTRGHMGTRTTRTDFFHVVTVGITKPF"
     gene            complement(8267..9394)
                     /locus_tag="VMZ53_04260"
     CDS             complement(8267..9394)
                     /locus_tag="VMZ53_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS27691.1"
                     /translation="MSHDTRDKPADDRIAELELELAAAQRTIEVLIARLERAGTTPPA
                     QAELFDAAVRLGHVLEERTREVEAARAEFRALRANLDQIVKQRTRALAESELQLRTKN
                     VELERQGRIKQEFISIAAHELRTPLTSIVGYLDLFSEGRFGELPPLLARPMSSVRRNA
                     HRLKRLVDDMLSVSRLEAGTLVLRREPVDLGDVVRAVVTELLPLAQARKQEIDVQLEP
                     LGPLDGDRDKIHQIAVNLVSNAIRYTPESGKISVNVDEAPRERYVGGWARLRVRDNGV
                     GISEEDRQRIFEGFLHAQPAKHHTSAGPDSAGLGLYIARGLIELHGGLITVDSEPDVF
                     TEFTVLLPFKQQRTRPASSDPPLPTSETDTAKASTSSSTSS"
     gene            complement(9420..10574)
                     /locus_tag="VMZ53_04265"
     CDS             complement(9420..10574)
                     /locus_tag="VMZ53_04265"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HUS27692.1"
                     /translation="MDGIARSVTARGFEAERVASELVSGLRPTNGDDLRLVVAFVDWR
                     IDTAAFARSMQQALPAPVVGCTTIGVIGGNGEAPSAAAIGFYGDWVRVGIGIATELPK
                     SALARSRDAVHAAATALGTTAHALDSARHVGFTLVDGSSGHEEAFCIASAATAPQIRV
                     VGGSAATEVGAKDRRAYVFANGEALADAGVVVLLETKRPFEAITSQHMVATAVKTVVT
                     AAAGRAIVELDGRPAAERLRELVAELGGTIDPTTPSEFAFARFVDGVPYLRSIVGIQD
                     RRVLLACAVEEGHVLRLMRPGDLIGQTQKDLAATAERIGGKISALLAFSCIGRHWEAA
                     ARGVDKKLAEAYAAYPTIGFQSFGEQTGMLLVNHTLTGLAIGAAPPAADE"
     gene            10645..11601
                     /locus_tag="VMZ53_04270"
     CDS             10645..11601
                     /locus_tag="VMZ53_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HUS27693.1"
                     /translation="MEKTCSACGQVFRPAFVFQIAVTADGQKSHYCSLECRKTGLGEE
                     GFRAKRARRIAILNQKGGTGKTTTAVNLAAGLAEKEQQALLIDTDAQGNVGVSLGVAG
                     EKSLYHVLVESAEPTEVAVPVRSHLDVITSDATLAAAEIWLARQNNDSRSRVLTKRLN
                     MLQISRRYDYIIIDCGPSLNLLNQNALSYVDEVIIPVTCDYLALVGVKQVLRTIKDVE
                     RHLGHAVRISAVLPTFYDGRTRLAREVLETLQGHFKTKCLEPIRLNTRLAEAPSHKKT
                     IFEYAPVSHGAEDYNRVVDWVLSSNAVASTATTLHSEPQVAV"
     gene            11598..11936
                     /locus_tag="VMZ53_04275"
     CDS             11598..11936
                     /locus_tag="VMZ53_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27694.1"
                     /translation="MKEDKLMAHDPLADADAILSEEYYGRRRVGDAPASADGRKTRHA
                     GSAAAANGKARPTHYKVICISMYTRDLEDLDAKVAELKKRGWTKANKSQLIRLALSQI
                     DIDKLPTPRQ"
     gene            11933..12229
                     /locus_tag="VMZ53_04280"
     CDS             11933..12229
                     /locus_tag="VMZ53_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27695.1"
                     /translation="MIEDLADRYHEYLERWKRNAGDVPVGAFTKFQGKLIKKLSFEEF
                     SPILLEYVEMVTRYEESVERGDTINDVVLKVLRDQAAQLMLTPPSADVKSTPEI"
     gene            12264..12938
                     /locus_tag="VMZ53_04285"
     CDS             12264..12938
                     /locus_tag="VMZ53_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27696.1"
                     /translation="MKQFVQGFLIASLVATPLFIAGCKKKTDEASTGSATTTTTTTTT
                     TTTTAGSGGSAATATGSGSATAAAGSGAGSAAAAAPTSTDPAGAAKVTAKRGGKDEGT
                     FDKALQANRGSAGYRVYIASKCDKFNCGHIDAKGYTDDTILQKDCPDAKVMTIGFNFD
                     ADPKAGDVEVSVEVNGFSSGGSITSNWTKGGKISALSDKEVKGSLDVKDGDGNAKGDF
                     TATVCK"
     gene            complement(12987..>13424)
                     /locus_tag="VMZ53_04290"
     CDS             complement(12987..>13424)
                     /locus_tag="VMZ53_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27697.1"
                     /translation="RARRSQPRRRRSNGSPRAERRETEASQPGTAVEETRRRLSFRRA
                     TAVRDPRRRESTSAGVVVSAPRLAALAALTTALVAPTAVETASRRRLSFRRRAPRPRR
                     PTAEETRWRLSFRLDRDLSLMPGTEEVRLYVRSVVTLYGIGVQ"
BASE COUNT         2321 a         4372 c         4494 g         2237 t
ORIGIN      
        1 gagctgccac aaaacggctt cgtctcgagc gcgctcgtcg accaatcgtt gaatgtaccg
       61 gcgacgtgcg tcggcgcttg cgtacgaaac agcgctcgac cttccgccca gagcggcccc
      121 aggctcgcgc gagcatcgag ctcggtctgc agatcggtga ccgccttcgc atcgcagccc
      181 gcagccgcct gatcgtagag cgcgagcacg cagggagcgc cggccttgcc gggagcgcag
      241 cccgtcgggc tgtcgggcat gctggcatca ggttccgaat cgtcgtcgga catctggccg
      301 ttgccgtaat cgcaggcggc tagtagggcg agaagggaca gggcaatgcg catggcgttg
      361 tgttcggcaa cgcggcgaga cgtaacgtcg cggactcgcg tggctggcgg cggatacagc
      421 aaggggtggg cctgcaaatg ggctagaacg gacgggtgtt gcagacgccc tcgcgcaggc
      481 tctggatcct tgcgatcgtc gtgagcgtcc tctgcgtcgg cggtctcgtc tatgcgctga
      541 tcgtcgagcg cgacacaccc gcggagctgc gaagcacgac aggccccgtc tcgtccggcg
      601 gcaacagcgg cttcgcgctc ggcgtcctcg tcggtgtcgg cgcgggcatc gtgatcggct
      661 ctctgatcgc gctgcgcagg cgcagctagc ggcgatcgcg ccactcatcg acgagcatgc
      721 cgaacatgtg cacgtcgcac agccgcttgc ctttgcggac gtgcgcgcgg cgaatgcctt
      781 cgctcagcat gccgagcttc tcgagcacgc gacacgacgc atcgttgccg tcgagaacct
      841 gcgcgtagat ccgctgcagg ccgagctggc cgaagccgag gtcgatgagc gcgtccgacg
      901 cttcggtcgc atagccttgg ccccacgcat ccatgccgag ccagtagccg agctcggcgc
      961 gccgatcgcg gatgttgcgg cgaagcgaga ccgtgccgat gagctggttc ggagcgccgt
     1021 gcttcgtgat cgcgagcgtg agcccacgcg cctgcggcca ccacgcgatg cgtgctgcga
     1081 cccagcgagc ggcgagcgcg acggggtagg gactcggcac cgcgatcaga tactgggcga
     1141 cgttcttgtc tccagcgcgc atcgcgatcg agtgcgcgtc ctcgatgcgg agctcgcgca
     1201 gggcgaggcg ctcggtgtgg agcaccggcg ttgcgggacc tatgtccgcg agcgcgatca
     1261 cgtcgtcgtc attttggtgg gcgcgagccg ctgaactctt gcacgctcgt gcacacgatc
     1321 gcggcgcagt cggtgttgtc gttaccgttc gaggcgtagc tcaacgtgcc gttgagagca
     1381 ttgcccgtca gcgtcgcttg gatgcggccg tcgtaggtgt acgtgcagtt gccgctcgtc
     1441 tgcggccggg tgcccgaaag cgacagatcg agctcgtcgc cgtcgaccga tccggagaac
     1501 gtgttcgagc caaacgcgaa gttgaggtac ccgcccgcga tgccttgaac ctcggcgatc
     1561 gcgttcacgt cgttttgcgt gatgaccacc gtgacgccgg acgtcatctc cccgacggtc
     1621 cagttttgga agttgcatcc attttcgcgg ttcgtcaggc cgaccgagta ggtgccctcg
     1681 acgtcgaccg gatcgccacc gcacgcagcc atagccacaa ctaggagtag gcgcttcatg
     1741 ggctccagga taaccaaaat cccgggcact tggcctatgt tctagaaacg tgcccggttc
     1801 ttccacccgt gcctatcatc acgctgctgc cgccctagcg atcacgctcg gcgcgggacc
     1861 tgcgagcgcg gccgtgcacg acgtcgagac tcatcgcctg gacaacgggc tgactttgca
     1921 tgtggcgggt ggtcacccag cgccggtcgt cgccgtgcag gcatgggtcg gcgttggctc
     1981 ggccgacgag cagccgccgc aagccggtgt cgcgcatgtc gtcgagcaca tgctgttcaa
     2041 gggctcgtcg agctatggcg tcggcgagct gacgcgcgcg atcgaaggct cggggggcga
     2101 gatcaacgcg tggacgtcgt tcgaccacac cgtctatcac gcggtgctcg ggcgcgatca
     2161 cctcgacggt gcgctcgacg cgatcggcga cacgctcatg gcgccgcgcg tggatcccga
     2221 cgagctcgca cgcgaacgcc gcgtgatcct cgaggagatc cggcaaggca ccgacgatcc
     2281 ggcgcgctcg gtcgcgcaga gcctgttcgc gacagcgtac gtggcgcatc cctaccggcg
     2341 tccggtgatc ggcacggcgg agagcgtgca gcgcctcggt gagcgcgacc tcgtggagtt
     2401 tttccgcagc tactacgtcg ccgacaacgt cacgctcgtc gtcgccggtg atgtcgaccc
     2461 ggcgcgcgtg attcgctccg tcgagcgacg cttccgcggg atgccatcgg gccggccgac
     2521 gcggcgcatt ccacacgagc ctgcgcagac ggcacctcgc gcggtcgctg cgcatcgcga
     2581 cgtcagcgag gcgtacctcg ccgtcggctt tcacgtgccc gctgcccggc atccggacgt
     2641 cgctgcgctc gatgtcgcgg cgatgctcct tggcgagagc gaatcggcac gcttgcctcg
     2701 aacgcttcgc gatcgcgacc agctcgtcac cggcgcgtac gcccacgtgc acgcgctgcg
     2761 cgaccccggc ctgttcgtgc tctcggcaac ggcgcgcgca cgcgatgccg agaaatccgt
     2821 cggcgcgctc gtcgatcaag cgctcggcct cgtcaacgat ctctcgcaag acgagctcga
     2881 taaagcacgc atcgccgcgg aggccggcct cgttcgccag ctcgagaccg cgcagggccg
     2941 cgcgcgcgtg ctcggctgga acgcgtcgat gaatggcgat ccgcgcttcg cgcacgtcta
     3001 cctcgatcgc atccgcgcgg tgcgtcgcca cgacgtcgct gctgccgtcc accgctactt
     3061 ccgctccgag aacgcgacgg tcgccgcgat cctgccgaaa ccgacgcgcg cctcgacgaa
     3121 gacccacgcg acgttcgccc gcggcgccga gaaacgcgtc cgtagcgcac tgcgcatcgc
     3181 caccgcaccg acgacgtccg cagaaaagcg cgttgtcctg caaaacggca tggtgctgct
     3241 cgttcgccgc gatgcttcgg tgccgatcgt cgcgatgcgc gcggtgtggc gcggtggcca
     3301 gcgcatcgaa gacgcggatc acgcgggcgc ctcgacgttg ctcgctcgcc tcatcacgcg
     3361 cggctgcggc cagctcgacg ccaaccaagt cgccgatcgc gtcgatcgcc tcggcggctc
     3421 gctcggcggc gtgtccggac gcaacagctt cggcctcgcg tcggagtggc tcgcgaagtc
     3481 gtggcgcaac ggcttcgatc tcattgccga ctgcctgctc gagccgcagc tcgcggccga
     3541 ggagctcacg cgcgagaagc gcgctctcta cgacgatcag accgcgcagc aagacaaccc
     3601 gacccaggtc gcgttccgct tgttcagcga gacgctctac ggctctcacc cgtacgcgcg
     3661 tgacgtgctc ggcaccgccg acgcgatcgg cgatctcgat cgcacagcgc tcgtcgcgtt
     3721 ttacaatgac caatatccga tctcggcgct cacgctctcg atcgtgggtg acgtcgacgt
     3781 cgacgatgtg atagcgcgcg tgacgaagcg cttcggcgat ctgtcgtcga agaaaccgcc
     3841 gtcgccgtcg gtggcgctgc cgtcgttcga caaccggccc gccgcgaagc gcgaggtcta
     3901 tcgctttctc gatcgcgcac aagcgcacct cgtgatcggc tatccgggcg cgaccgtcga
     3961 cgcaaaagat cggttcgcgc tcgaggtcct catcgcgatc ctcggcggcc agagcggtcg
     4021 cctcttcagc gagctgcgcg acaagcaggc gctcgtctat cgcgtgagtg cgcactccgt
     4081 cgaaggcgtc gacccgggct tcgtcgccgt ctacctgtcg tgcgcgcccg cgaacctcga
     4141 agccgcgatc acctccgtgc gcggcgagct caccaccgtc acgaaggacg gcgcgatcac
     4201 cgcggacgag ctcgagcgcg cgaagtcata cctcgtcggt agccatcaga tcgcgatgca
     4261 gcgccgctcc gctgtcgcaa acgcgatcgc ctatcacgag gcatacggcc tcggctggaa
     4321 gacgtgggcg agctacgacg acgcgatcaa ggcagtcacg ctcgacgatg tcgcggcggc
     4381 cgctcgcacg tacctgcgcg acgatcgcgc gatcaccgcc acggtgcgcc cgccgctcga
     4441 gacgccggcc gcggcgaaga agtcaaagct caagccgcag ccgccgaagc tgaaggcgcc
     4501 cgagcccgcg aagaaaccgc gcaagaagcg gtaaccgttc ccgcgcgacc tgaaatgtgg
     4561 cgcctccgcg acgacgcggc gatcctttgc acgtcgtgag tcggcagcgt tcgaacaccg
     4621 cgggttgggc ggggcatggc gatctcgagg ccagcctcgt cctgatcttt cccgtgctcc
     4681 tcgcgtacga ggtcggcgtg ctgttcggcg ggcgcgtgaa tggcgcggac gtcatcacgc
     4741 ggctgctcta cacgctgctc ggcagcaaga cgctgtacct gctcgtgcat gcggtcgtcg
     4801 cgctgtggtt tctgctgtgg gcgcgtgcgc ggggcaagtg gggcacgctg cgcatggaga
     4861 tcgtcgcgcc ggtcgtcctc gaagctgcga tctacgcgct cacgctgggc gctgtggtga
     4921 acctcgtcct cgagaagctg ctcggtctcg gcgtcggtcg cgagtcgatc atctccgcgc
     4981 tcggtgccgg cgtgcacgaa gagatcgtgt ttcggctcgg cctgttcgcc ggcctcacat
     5041 cgctgctcga gagccggcag cagcggttcg cgatgcccgt cgcgctcatc gtgtcgtcga
     5101 tcgcattcgc agcggcgcat cacctcggcg cgcatggcga gccgttcacg atgcacgcgt
     5161 tcgcgttccg tgcgctggcc ggtgccgcgt tcggcgcgat cttctggtac cggtcactcg
     5221 cgcatgcggt ttacgcgcac gtgctgtatg acctcgtggt cgtcgcgacg agctagctac
     5281 tcgaagccga gcgagccgcc gccgtcgagc tccatcttgc cgacgtagag atcgaacttc
     5341 gcgcgcttga tctgatcttg gttcgccgtg ctcgcgacga cgttcgtgcc gatgaggccg
     5401 acttccgaca ggttgatgcg gaacagcgtc cgcgacttat cgggcgagag gaactcgagc
     5461 gagccggtgc gttgcgcgcc gggatccgcc ttgcccttga gcacgtagtc gttgtgccac
     5521 tcgaggagct tgtcggcgta cgccatcgac atcgtgcccg agatgttcgg gaactcgatc
     5581 ttcgtcggga tgatctcggc gaggcgctgc gcgccggtct gcatcttctt gacgccctgc
     5641 ttgatcgtga acgactcgat cttgttgcag tacttcatct cgtcgatgcc gtcgatgttc
     5701 aggcggaagc ccgagcacaa ccacatcttc tgcttgcctt tgtcgatgcc gcggagcgac
     5761 ttgccgtcgc ccttcttcgt catcacgcct tcgggctgga acttgacctt gatgtacgcg
     5821 gcgtccttcg acgcgccgtc gagcgcgggg aacgtggtct cgaggatcaa cgcgtcggag
     5881 aagtgatgct cgagcttcga ggtgttgttg aagtcgccgt gcgtgatctg accgctgcgg
     5941 cgggtcgggt tcttgttcca cgactcctgg atccacttga gcacgctgtt cgcgcccgag
     6001 aggccgaagt cgaacgtgac cggatcgatc tccaacaacg aggcatgctt gattcgcatg
     6061 ttgttgggac caatggcctc gtcgatgacg gagtgcttgg cccaaccgcc gtcgatcgac
     6121 tttagaaacg ccgtggtcgg cgccccatcg atgatcagct cgaagtgacc tgccgtgtac
     6181 gaatctttgg ttagcaagtc tcgaatgccc atagagcgac cctcagctta gcgctttcct
     6241 tctacgatta ctggcgcctg gatccccccg cgggcactgg agtccccaga aggcacctgc
     6301 gaaatcgacg taatttcgag cgattggatc cggcacgtcg cttgtacaat aatgacgtca
     6361 tgagggtcgt tgccctgatc ttgctcgcgt tcggcttgct cgtcggaatc ggcggctctg
     6421 ccgacgccga gaaggtgaag accaaccaat cgacgaaggt ctacgcgaga cccggcgagc
     6481 aggcgaaggt catcgtcacc gtcaagtccg gtcagaacat gacgctgctc gcgaaggacg
     6541 gacgctggct caaagtccgc gtcaatggcc gcaccggcta cgtgcctcgc agcaaagtcg
     6601 atatggccga cgacgacgag atcgcgcgca acacgcgccg ccgtccgttc gtcgatggtc
     6661 gcggccgccg tcgcggcttc ggtggcgatg cccccgatga tcgcgtggcc gcagacgcca
     6721 caggcgacgg cgccgacagt ggcgacgatg aagaagaaga cgaagaagag gacgagccgg
     6781 ccaagaagcc ggtgaagacc gccgccaagt cgaatgccaa gaaaggcggc gacgaggacg
     6841 aagaagaaga ggaagacgaa gaagacgaga agccggtgaa gaaggctccc gtgaaggtcg
     6901 ccgtgaaagc caccgagaag aagacgcctg cgaagaaggg cggcgacgag gacgaagaag
     6961 acgaagagga cgaagagccg gcaaagaccg cggcgaaggc cggtggtgac gacgaagaag
     7021 aggacgaaga gacaccgacg aagggcggcg acgaagaaga gaaagcagac acgcgcccgg
     7081 tcgctcgcgt cggcaagaag gtcgcggtct acgagaagcc gaacctcgat tcgaacgagg
     7141 cgttcgtcgt acgtccgagc gatctgctgt atccgtcgga cgagaagaac ggctggacgt
     7201 tcgtcgagaa cagcgaaggc gatagcggct ggatcgaatc gaccgagctc tcgatggagg
     7261 gtggcggcgg tggcggcggc atgaagaaga cgatcgatgt gcgcgcgcgc ttcggcctga
     7321 tgttcctgca acaaggcgta cgcacgcagg gctcgacgaa cctgaacgtt ccggacaact
     7381 acaacatcgg cacgtcgtcg atcgcgatgg cggtcggcgc gggcatcttg atcccgaagg
     7441 gcaagaagct gctgctcggc ggcgaattca cgtacgacta cgcgaagacg ctgctcggcg
     7501 gtgtgttcta cgacgcggac ggcaagggtg gcagcccggg cgtgaacatc ggcgtcacga
     7561 tccacaacat caatgcgcgc ggcatggtcg gcatcgatct caagaagaag agcggcatgg
     7621 cagtgttcgg ccgcctcggc tatcgctacc aaggcttctt gatcaccgac gtcgcgagca
     7681 tgacggcaaa ccctgcaaag ctgccgtcgg aagtcgtgaa ggcgccgacg atcggtgcag
     7741 cgctcgcgat cccgaagctc agcgagaagc tcggcttgcg tctctcgctc gacgcgatcg
     7801 cgttcggcgc gagcgtgaag caaaccgtcg gcctcgagga cggctcggcg ccgagcgtga
     7861 aggccgtcga cgtcggcgcc gggctgacct acaagttcaa gaaggcgttc gacatccaag
     7921 ccgcgtacga cttgcagtac atgggcatcg acttcggccc gccgctcatg tcgtcgacgc
     7981 gtgggcacat gggtacgcgt acgacgcgca cggacttctt ccacgtcgtg acggtcggta
     8041 ttacgaagcc gttctgaccc ttcgtagtcg tggtcgtggt cgtggactga agcagtcgca
     8101 ggcgctagct acgtgggcag ggcgtgttgc cccgcatggt ttaacacgga gacacggaga
     8161 cacggaggcg ccgaggaaag gtccggcagg acggttcggg tgccggccat ggtgtcgacg
     8221 gagccgcaga gtcgggtgcc gtccacgtcg ccgtcgccgt caaggttcac gacgacgtcg
     8281 acgatgacgt cgaagcttta gccgtatcgg tctcgctcgt cggcagcggc ggatccgacg
     8341 atgcgggacg cgtgcgctgc tgcttgaacg gcaggagcac ggtgaactcg gtgaacacgt
     8401 cgggctcgga gtcgacggtg atgaggccgc cgtgcagctc gatgaggccg cgtgcgatgt
     8461 agaggccaag gccggcggag tcgggaccgg cgctcgtgtg gtgcttcgcc ggttgcgcgt
     8521 ggaggaagcc ctcgaagatg cgctggcgat cttcttcgga gatgccgacg ccgttgtcgc
     8581 gcacgcggag gcgcgcccag ccgccgacgt agcgctcgcg cggcgcctcg tcgacgttga
     8641 cggaaatttt gcccgactcg ggcgtgtagc gaatcgcgtt ggagacgagg ttgaccgcga
     8701 tctgatggat cttgtcgcga tcgccgtcga gcgggccgag cggctcgagc tggacgtcga
     8761 tctcttgctt gcgcgcttgg gcgagcggca gcagctcggt gacgaccgcg cgcacgacgt
     8821 cgccgagatc gacgggctcg cggcgcagca cgagcgtgcc cgcttcgagg cgcgacacgc
     8881 tcagcatgtc gtcgacgagg cgcttgaggc ggtgcgcgtt gcggcgcacg gagctcatcg
     8941 gtcgcgcgag cagcggtggt agctcgccga agcggccctc gctgaagagg tcgaggtaac
     9001 cgacgatgct cgtgagcggc gtgcgcagct cgtgcgcggc gatcgagatg aactcctgct
     9061 tgatgcggcc ttggcgctcg agctcgacgt tcttcgtgcg cagctgcagc tcggattcgg
     9121 cgagcgcgcg tgtgcgctgc ttgacgattt gatcgaggtt ggcgcgcagc gcgcggaact
     9181 cggcgcgtgc agcttcgact tcgcgcgtgc gctcttcgag cacgtgaccg aggcgaacgg
     9241 cggcgtcgaa cagctcggct tgcgcgggtg gcgtcgtgcc ggcgcgctcg agacgcgcga
     9301 ttagcacttc gatcgtgcgt tgcgccgccg cgagctcgag ctccagctcg gcgatccggt
     9361 cgtcggcggg tttgtcgcgc gtgtcgtggc tcatcgtggc tgctcgttgc tactcgtcgc
     9421 tactcgtcgg ccgccggcgg cgcggcaccg atcgcgagcc ccgtgagcgt gtgattgacg
     9481 agcagcatgc cggtttgctc gccgaagctc tgaaagccga tcgtcgggta cgcggcgtac
     9541 gcctcggcga gcttcttgtc gacgccacgc gcggcggctt cccagtggcg accgatgcag
     9601 gagaatgcga gcagcgcgga gattttgccg ccgatgcgtt cggccgtcgc cgcgagatct
     9661 ttttgcgtct ggccgatcag gtcgccgggc cgcatgaggc gcaggacgtg gccctcttcg
     9721 accgcgcagg cgagcaggac gcggcggtct tggatgccga cgatcgagcg caggtacggc
     9781 actccgtcga cgaagcgcgc gaatgcgaac tccgacggcg tcgtcggatc gatcgtgccg
     9841 ccgagctctg ccacgagctc gcgaaggcgc tctgcggccg gcctgccatc gagctcgacg
     9901 atcgcgcgtc ccgcggccgc ggtgacgact gtctttaccg cagtcgcgac catgtgctgc
     9961 gacgtgatcg cttcgaacgg ccgcttcgtc tcgagcagca ccacgacgcc ggcatcggcg
    10021 agcgcttcgc cgttcgcgaa cacgtatgcg cgacgatctt tcgcgccgac ctcggtcgcg
    10081 gccgagccac cgacgacgcg aatctgcggc gcggtcgcag cggatgcgat gcagaacgct
    10141 tcttcgtggc ccgagctgcc atcgacgagc gtgaacccga cgtggcgtgc cgagtcgagc
    10201 gcatgcgccg tggtccccag cgcagtcgcc gcggcgtgca ccgcatcgcg gctccgcgcg
    10261 agcgcggatt tcggcagctc cgtcgcgatc ccgatgccga cgcgcaccca atcgccgtag
    10321 aagccgatcg ctgcagcact cggtgcctcg ccgttgccgc cgatcactcc aattgtcgtg
    10381 cagccgacca ccggcgcagg cagtgcctgc tgcatggacc gcgcgaaggc cgcggtgtcg
    10441 atgcgccagt cgacgaatgc gaccaccaat cgcaggtcgt caccgttcgt ggggcgcaaa
    10501 ccactcacga gctcgcttgc gacgcgctct gcctcgaacc ctcgtgccgt cacactccgc
    10561 gcaatgccgt ccatgcggcg caaactctag cagcgcgcgg ctgaaaagtt gcccggatta
    10621 cgcgcatgcc gcttcgataa gggcatggag aagacgtgta gcgcgtgcgg gcaggtgttc
    10681 cggccagcgt tcgtgtttca aattgcagtt acagcggacg gccagaagag tcactactgc
    10741 tcgctcgagt gccgcaagac cgggctcggc gaagaaggct ttcgcgccaa acgcgcgcgc
    10801 cgcatcgcga tcctgaatca gaagggcggc accggaaaaa ccacgacggc cgtgaacctc
    10861 gccgcaggtc tcgccgagaa agagcagcaa gctcttctca tcgacaccga cgcgcagggc
    10921 aacgtcggcg tttcgctcgg tgtcgccggc gagaagtcgc tctatcacgt gctcgtcgaa
    10981 tcagcggagc caaccgaagt cgccgtgccg gttcgctcgc acctcgacgt catcacgtca
    11041 gacgcaacgc tcgccgcagc ggagatctgg ctcgctcgcc agaacaacga ctcgcgctct
    11101 cgcgtcctga cgaagcgcct caacatgctg cagatctcgc ggcgctacga ctacatcatc
    11161 atcgactgcg gcccgtcgct gaacctcctc aaccagaacg cgctgtcata cgtcgacgag
    11221 gtcatcatcc ccgtcacgtg cgactacctc gcgctcgtcg gcgtgaagca ggtgctgcgc
    11281 acgatcaagg acgtcgagcg ccacctcggt cacgccgttc gcatcagcgc ggtccttccg
    11341 acgttctacg acggccgcac gcgcctcgcg cgcgaagtcc tcgagacgct gcagggtcac
    11401 ttcaagacga agtgcctcga gccgattcgt ctcaatacgc gtctcgctga ggcgccgtcc
    11461 cacaagaaga ccatcttcga gtacgcgccg gtctctcacg gcgccgaaga ctacaaccgt
    11521 gttgtcgatt gggtgctctc gtcgaatgcc gtcgcgagca ccgcaacgac gcttcattcc
    11581 gaacctcagg tggccgtatg aaagaagaca agctcatggc gcatgatccg ctcgccgatg
    11641 cggacgcgat cctctccgag gaatattacg gtcgtcgccg cgtaggcgat gcaccggcgt
    11701 ctgccgacgg acgcaagacg cgtcacgctg gctccgctgc tgccgccaac ggcaaggcga
    11761 ggccgacgca ctacaaggtg atctgcatct ccatgtacac gcgcgatctc gaagacctcg
    11821 atgcaaaggt cgccgagctc aagaagcgtg gctggacgaa ggcgaacaag tcgcagctca
    11881 tccgcctggc gctgtcgcag atcgacatcg acaagctgcc aacgccgcgt cagtgatcga
    11941 agatctcgcc gaccgctatc acgagtatct cgagcggtgg aaacggaacg caggggacgt
    12001 tccggtcggg gccttcacga agttccaagg caagctgatc aagaagctca gcttcgaaga
    12061 gttcagcccg atcttgctcg agtacgtcga gatggtcacg cgctacgagg agagcgtcga
    12121 acgtggagac acgatcaacg acgttgtcct caaagtgctc cgtgaccagg ccgcgcagct
    12181 catgttgacc ccgccgtcag ccgacgtgaa atcgacgccc gaaatctgac atcgagcccc
    12241 gggcgtgatt tgcttcgcgc cctatgaagc aatttgtgca ggggttcctc atcgcatcgc
    12301 ttgttgccac gccgctgttc atcgcgggct gcaagaagaa gacggacgag gcgtcgacgg
    12361 gctcggccac gacgacgacg acgaccacga cgacgacgac gacaacggcg ggctcgggag
    12421 gttcggccgc gacagcaacg ggctcgggtt ccgcgacagc tgcggccggc tcgggcgcag
    12481 gctcggctgc tgctgctgct ccgacgtcga cggatcccgc cggcgccgcg aaggtcaccg
    12541 caaagcgcgg cggcaaggac gagggcacgt tcgacaaggc gctccaagcc aaccgcggca
    12601 gcgcgggcta ccgcgtctac atcgcgagca agtgcgacaa gttcaactgc ggccacatcg
    12661 acgcgaaggg ctacaccgac gacacgatcc tccagaagga ctgccccgat gcgaaggtga
    12721 tgacgatcgg cttcaacttc gacgcggacc cgaaggctgg cgacgtcgag gtcagcgtcg
    12781 aggtcaacgg cttctcgagc ggtggcagca tcacgagcaa ctggacgaag ggcggcaaga
    12841 tcagcgcgct ctcggacaaa gaagtgaagg gctcgctcga cgtcaaggac ggcgacggca
    12901 acgcaaaggg cgatttcacc gcgacggttt gcaagtaacg ccgcgcctgc cgacgtgaag
    12961 tccacgccgg cccgccagac ccacagtcac tgcacgccta tgccgtagag cgtgaccacg
    13021 ctgcgaacgt acaggcggac ctcctcggtc cccggcatca gcgagagatc gcggtcgagc
    13081 cgaaacgaca aacgccaccg agtctcctcg gcggtggggc ggcgagggcg gggagcgcgt
    13141 cgccgaaacg acaggcgccg ccgggaggct gtttcgacgg cggtgggggc gacgagcgcg
    13201 gtagtgaggg cggcgagcgc cgcaaggcgc ggcgccgaaa cgacaacgcc cgccgaggtg
    13261 ctctcacgcc gccgggggtc tcgaacggcg gtggcccgcc gaaacgacaa acgccgccga
    13321 gtttcctcga cggcggtgcc gggctggctg gcctcggtct cgcgcctttc ggcgcggggt
    13381 gacccgttac ttcttcttct tcttggctgg cttcgccgcg ccct
//
LOCUS       DATLBZ010000079         3399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9722019, whole genome shotgun sequence.
ACCESSION   DATLBZ010000079 DATLBZ010000000
VERSION     DATLBZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9722019"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..695)
                     /locus_tag="VMZ53_04295"
     CDS             complement(108..695)
                     /locus_tag="VMZ53_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS27698.1"
                     /translation="MRVLALMLCALAACDNAFGLDRLHTSPLVDSASSQIDVVASNCP
                     ADYDQTLPESQSHYRIVGTLAKWIPAADDCADDLAGQTHLIVVSNQAELDALRGLASI
                     FLPDDTWAGASAIKLGTNTMVWVTDEDTHGFIVPTVKGVLPWESDQPDEGAGAAEMRG
                     INDPAPGNLHDEKKDSTSRYICECDGRANNEATYR"
     gene            727..1503
                     /locus_tag="VMZ53_04300"
     CDS             727..1503
                     /locus_tag="VMZ53_04300"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HUS27699.1"
                     /translation="MDVERAREVIAAEKPRRLRLDEMLRDMGARLPERKKKATRRSKP
                     GIEPRASQQGIDPRASDAGISDDGMPSPDTTLDERRPITMGELVDRAAEAGFGFLIGV
                     LALIAIPFFGLSTPFGLAIALVGGQLAIGRKRPWLPKRTRRRVLAMRMLDRVLGLLDR
                     RLRWLAQSTRRRWEVLITPRLVGVGITFLALGLALPLPIPGSNLIFIVPILIYAIGLL
                     ERDGKWVVVGHICTLVDLALLIAFGATVVAVLERAWQWVF"
     gene            complement(1500..2132)
                     /locus_tag="VMZ53_04305"
     CDS             complement(1500..2132)
                     /locus_tag="VMZ53_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS27700.1"
                     /translation="MRIVVVLSALLVATGCDGVFGLTRLHEHASDDDASVDALGDGAP
                     SDTPGDAKMIDAYVQKTCAELGYTLQVSSGSSTRYRNGGTTKKTWLNAELDCADDASA
                     ANNVHTHLVVLSNDQEAGGIHIDVLGSADAGGYWTGLTDRAAPGTYRWVTNEGPGYPP
                     ASGSPWGTGQPNGSGGCVVFDNFSMFDVSCIETHFFACECDAFAEDTTHF"
     gene            complement(2186..2689)
                     /locus_tag="VMZ53_04310"
     CDS             complement(2186..2689)
                     /locus_tag="VMZ53_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HUS27701.1"
                     /translation="MALSVLPLDLARWDEAARDCLVLPVFRDDRPLRGAAGLADWRLC
                     GRLSRLLKSEKATADAGETMLLPPGRRLRFKRVLWFGLGDAKGYSDDRCRKDLTWILG
                     VVKNAGVTDWALQAPGRASGLIGARRAVEIILEDEQLSAQPITLLEDPAGQKDIAELL
                     RSQRQAG"
     gene            complement(2689..3123)
                     /gene="nusB"
                     /locus_tag="VMZ53_04315"
     CDS             complement(2689..3123)
                     /gene="nusB"
                     /locus_tag="VMZ53_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HUS27702.1"
                     /translation="MTAGVRRTGRAYALQLLYARDGDASTDVTSAAFAWAGDFELEIE
                     PPAQQFAKDLVSAATDRAPEIDELITSSSKNWRIDRMSRVDRNILRLGACELLAFKDV
                     PVKVVINEAVELAKRFGTAESSAFVNGVLDRIATAVGRTAED"
     gene            complement(3120..>3399)
                     /gene="ribH"
                     /locus_tag="VMZ53_04320"
     CDS             complement(3120..>3399)
                     /gene="ribH"
                     /locus_tag="VMZ53_04320"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HUS27703.1"
                     /translation="ERGGIDAVVALGAVIRGSTPHFDYVAGEASRGIASAASADIPIS
                     FGVLTTDTIEQALERAGTKAGNKGFDAAMAAIELAQLFRALSGRKGKK"
BASE COUNT          532 a         1140 c         1106 g          621 t
ORIGIN      
        1 cgaacatcgc ccgccgaaca tcgcccgccg aacatcgccc gccgaacatc gaacatcgtc
       61 cggcgaacat cgtcggcccg tcagcgcgcg ccgagcatcg cgggccctta gcggtaggtc
      121 gcctcgttgt ttgcacgacc gtcgcattcg cagatgtagc gcgaggtcga gtccttcttt
      181 tcgtcgtgca ggttgcccgg cgctggatcg ttgatgccgc gcatctcggc tgcgccggcg
      241 ccttcgtcgg gctggtcgct ctcccacggc aggacgcctt tgaccgtcgg cacgatgaag
      301 ccgtgggtgt cctcgtcggt gacccacacc atcgtgttgg tgccgagctt gatcgcggag
      361 gcgccggccc acgtgtcgtc ggggaggaag atcgacgcga gtccgcggag cgcgtcgagc
      421 tcggcttggt tggagacgac gatgagatgc gtttggcccg ctagatcgtc ggcacaatcg
      481 tcggcggccg ggatccactt tgcgagggtt ccgacgatgc gatagtggct ctggctttct
      541 ggcagcgtct ggtcgtagtc cgctgggcaa ttgctcgcga cgacatcgat ttgcgacgac
      601 gcgctgtcga cgagtgggct cgtgtgcagg cgatcgaggc cgaacgcgtt gtcacacgcc
      661 gcaagcgcgc agagcatcag cgcgagcacc ctcaccgctc cgattctaca gtgctagggt
      721 tgcgacgtgg acgtcgagcg tgcccgcgag gtaatcgcgg ccgagaaacc gcgcagattg
      781 cggctcgacg agatgttgcg cgacatgggt gcgcgcctcc ccgagcggaa gaaaaaggcc
      841 acgcgccgat ccaagccggg catcgagcca cgcgcatcgc agcaaggcat cgatccacgc
      901 gcctctgacg ccggcatctc cgacgacggc atgccgagcc ccgacaccac actcgacgag
      961 cgccgcccga tcacgatggg agagctggtc gatcgtgcgg ctgaggcggg cttcggcttt
     1021 ctcatcggcg tgctcgcgct catcgcgatt cccttttttg gtctctcgac gccgttcggt
     1081 ctcgcgatcg cgctcgtggg tgggcagcta gcgatcgggc gcaagcggcc ctggctgccg
     1141 aagcgcactc ggcgtcgtgt gcttgcgatg cgcatgctcg atcgcgtgct cgggctgctc
     1201 gatcggcggc tgcgttggct cgcgcaatcg acgcggcgac ggtgggaagt gctcatcacg
     1261 ccgcgcctcg ttggcgtggg cattacgttc ctcgcactcg gcctcgccct gccgctgccg
     1321 attcccggct cgaacctgat cttcatcgtg ccgatcctca tctatgcgat cggattgctg
     1381 gagcgcgatg gcaagtgggt tgtcgtcggc cacatctgca cgctcgtcga tctggcgctg
     1441 ctgatcgcgt tcggcgcgac ggtggtcgcg gtgctcgagc gcgcgtggca atgggtgttt
     1501 taaaagtgcg tcgtgtcctc cgcaaacgcg tcgcactcgc atgcaaagaa gtgcgtctcg
     1561 atgcatgaga cgtcgaacat cgagaagtta tcgaacacga cacacccgcc actgccgttc
     1621 ggctggcccg ttccccatgg cgagcccgac gcgggcggat agccaggccc ttcgttcgtg
     1681 acccagcgat aggttcctgg cgccgcgcga tcggtcagcc ccgtccagta gccgccggcg
     1741 tcggcgctgc cgagaacatc gatgtggatg ccgccggcct cttggtcatt cgaaagcacg
     1801 acgagatgcg tgtgcacatt gttcgcggct gacgcgtcgt ctgcgcaatc gagctctgcg
     1861 ttcaaccacg tcttcttcgt cgtcccaccg ttgcggtagc gcgtcgagct tccgctgctg
     1921 acttggagtg tgtagccgag ctccgcgcag gtcttttgca cgtacgcgtc gatcatcttc
     1981 gcgtcaccgg gggtatccga cggagcgccg tcgccgagcg cgtccaccga tgcatcatcg
     2041 tccgaggcgt gctcgtgcag gcgcgtcagg ccgaagacgc cgtcgcatcc ggtcgcgacg
     2101 agcagcgcgc tgagcacgac gacgatccgc acgcgttgat cgtactctca tccggcgctg
     2161 tcgcgccgtg tcgcaccggc tgcgatcaac cagcctgacg ctggctgcgc agaagctcgg
     2221 cgatgtcttt ctgacctgcc gggtcttcga gcagcgtgat cggctgcgca gatagttgct
     2281 catcctcgag aataatctcg acggcgcggc gcgctccgat gaggcccgat gcgcggcccg
     2341 gcgcctgcag tgcccagtcg gtcacaccgg cgttcttcac gacaccgagg atccacgtga
     2401 ggtctttgcg acagcgatcg tcggagtaac ccttcgcgtc gccgagcccg aaccacagca
     2461 cccgcttgaa gcgcagacgg cgaccgggcg gaagcagcat cgtctcacca gcgtcggcgg
     2521 tcgccttctc actcttgagc agccgcgaga gccggccgca cagccgccag tcggccagac
     2581 ctgccgcacc gcgcagcggg cgatcgtcgc gaaagaccgg cagcaccaga cagtcgcgcg
     2641 ccgcttcgtc ccaacgtgcg agatcgagcg gcaggaccga gagagccatc agtcctcggc
     2701 cgtgcggccc acagcggtcg cgattcgatc gaggacacca ttcacgaatg ccgacgactc
     2761 ggcggtgccg aaccgcttcg cgagctcgac tgcctcattg atgacgacct tcaccggcac
     2821 atctttgaac gcgagcagct cgcaggcgcc gaggcgcaag atattgcggt cgacgcgcga
     2881 cattcgatcg atgcgccaat tcttcgacga cgacgtgatc agctcgtcga tctcgggcgc
     2941 gcgatccgtg gcggcgctca cgaggtcttt cgcgaactgc tgcgcgggcg gctcgatctc
     3001 gagctcgaag tcgcctgccc acgcgaatgc agcgctcgtg acgtccgtcg acgcgtctcc
     3061 gtcgcgcgcg tagaggagct ggagcgcgta agcgcgtcct gtgcggcgta cgcctgcggt
     3121 cacttcttgc ccttccgacc gctgagcgcg cggaacagct gtgcgagctc gatcgctgcc
     3181 atcgctgcgt cgaagccctt gttgcctgcc ttcgtcccgg cgcgctcgag tgcttgctcg
     3241 atcgtatcgg tcgtcagcac gccaaagctg atcgggatgt ctgcgctcgc cgcgctcgcg
     3301 atgccacgcg aggcttcacc ggcgacgtaa tcgaagtgcg gcgtcgagcc gcgaatgact
     3361 gcgccgagcg caaccaccgc gtcgatgcca ccacgctcg
//
LOCUS       DATLBZ010000080        11760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9006427, whole genome shotgun sequence.
ACCESSION   DATLBZ010000080 DATLBZ010000000
VERSION     DATLBZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9006427"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VMZ53_04325"
     CDS             complement(<1..816)
                     /locus_tag="VMZ53_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS27704.1"
                     /translation="MDRTLFSEEHLAFRKAFRQFADREIVPNQQKWREQGQVDREIWR
                     KAGAQGFLCTWMEEKYGGSGGDFLHATIIMEELARVYESGFAMGLHSDIIVPYIYSFG
                     SEEQKQKWLPGCASGEIVTALGMTEPGTGSDLAALATTAVKDGNEYVINGSKTFISNG
                     QLCDICVVAAKTDPNPENAHRGISLFVVEANRKGFQKGKRLHKMGMASQDTSELFFED
                     CRVPATNLLGVEGGGFMMMMQKLQQERLAVAIGAVASMEQVLQDTIAYTKERKA"
     gene            927..1166
                     /locus_tag="VMZ53_04330"
     CDS             927..1166
                     /locus_tag="VMZ53_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27705.1"
                     /translation="MGNGDHRKPESATPDLTVGRTTTLDDPLTTGLLAEVARSAQTKE
                     FDGDAIQQLIEQMQDDDTAVIIDKPVAHPNVRKRG"
     gene            complement(1163..2728)
                     /locus_tag="VMZ53_04335"
     CDS             complement(1163..2728)
                     /locus_tag="VMZ53_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HUS27706.1"
                     /translation="MFDFVIVGAGSAGCVLANRLSVGGRKVALIEAGGAQHKSLKVRA
                     PRLYQLLWSTPLDWAFKTVPQEHCAKRSHFWPRGKVLGGTSCLNAMVYIRGNRQNYDD
                     WAVPGWSYADVLPYFKKSEDNVRGASEYHGAGGPLHVSDNREPSGFAQAFVEAIAARC
                     KVAVNDDFNGADQEGAGHYQSTIKKGHRASTAIAFLEPALPRENLTVLSGAHALGLVL
                     DRDRVTGVRIRTTKGEQTIEGKEIILAGGAIGSPHLLLLSGIGPASELKAAGVEPKHE
                     LAGVGKNLQDHLLHAVNFEAASSPKLSKAAMLWWIARFAATSGGPLAGAALDAGAFVR
                     SSTSAPLPDIQFHVAPFGVKLPSDHGLVSPSFGRFACLLPGLIYPKSVGELRLASADP
                     AAPPAIDPKYFSDAADLELLVTGVKQAREIASTGALKQILGKEIYPGPGVASDDELRD
                     NIRAGCNTIFHPVGTCKMGTGPDAVVGPDLRVHGLRNLRVADASVMPKIIGGNTNAPT
                     IMIAEKCADLALA"
     gene            2755..3651
                     /locus_tag="VMZ53_04340"
     CDS             2755..3651
                     /locus_tag="VMZ53_04340"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUS27707.1"
                     /translation="MADTAWTVEQAGVTVAAFVKAKLGVPWTVAKRHVSTGKVSVDGA
                     VVTEIDHRLAAGQRVALQMSAPKPRDPEREGDLVYDDAHIVVIDKPAGVNSVPYEERE
                     SGTAMDLIRGAWRRMGKPATSVALHVVHRIDRATSGLLAFAKTKRAEIGMAAQLRAHT
                     MERMYICVTHGVMPAGRIESQLVADRGDGLRGSTSDPTRGKRAITHVTVRESLRKATL
                     CEVRLETGKTHQIRIHLAESGHPLVGETVYIRDYRGTPIDSPRLLLHAATLGFIHPIT
                     NEHVSLSSPLPPDFTAVVDRLR"
     gene            3657..4166
                     /locus_tag="VMZ53_04345"
     CDS             3657..4166
                     /locus_tag="VMZ53_04345"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS27708.1"
                     /translation="MVEDLIIRFAVPNDTGSILSFIRDLAEYERLLHEVVADEETLRA
                     TLFGVKPAAEVLIAERAGTPVGFALFFPSYSTFLAKPGLYLEDLFVRPAERGKGVGGA
                     LMAALARIAVQRNYGRFEWSVLDWNEPALKFYASLGAKPQSEWTVQRLTGDALYALAD
                     SWPTPVGKT"
     gene            complement(4121..4723)
                     /locus_tag="VMZ53_04350"
     CDS             complement(4121..4723)
                     /locus_tag="VMZ53_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HUS27709.1"
                     /translation="MLVRLGLETTHHHAPADEDRLAALTISSIHEYRSFLVRVLGFEA
                     PVEEAVAKVHQIELRFVRERARVPLLRRDLLALGMSERDIGECPRATVTIRTAPEALG
                     WMFVLERQTLLAGLLRRQIQQVLGTNTPTMYLNAYGGAPGARMRDFGTRLGELAHTQS
                     PSAIIDAANIAFRSQRQWYQQKDAADQVLPTGVGQLSASA"
     gene            complement(4735..5418)
                     /locus_tag="VMZ53_04355"
     CDS             complement(4735..5418)
                     /locus_tag="VMZ53_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27710.1"
                     /translation="MSEVHDEAIARGTGGHPARRMKSSWALTRVEKRYGARAETVASI
                     VAAPLIQPTRASYRRYLCDLYGFIVAFEARLAYAYTLDLRFIQERIKSGRMASDLLSL
                     GLSPYERVRLSQRCVVPRFDTAYKALGWLFVVERMMRELPAIRRRLCLALPSELEVAG
                     QFLGTPEIEIETSWDELGRVIDRHVHAQPELDATLLAVHAALQCLEEWVLLPATSSAS
                     VDTEQLRFP"
     gene            complement(5578..6183)
                     /locus_tag="VMZ53_04360"
     CDS             complement(5578..6183)
                     /locus_tag="VMZ53_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HUS27711.1"
                     /translation="MKDVSTALVRLNAATRQWHSAVDDSWLELLRPTVTVTDYLAQLV
                     RTYGLVAPFESACKYTPGLARVVVDLRRVGRAGLIAQDLLELDLTPSQVASIATCPEI
                     TMFATTREALGWLYVVERTTLLADGLLRHVRSSLPQVVNATRYLEAFSNDMSYWSRFG
                     RVIDDATVEPGAAAEIERAACEAFEAAHSWFRMSADARRTG"
     gene            complement(6307..6834)
                     /locus_tag="VMZ53_04365"
     CDS             complement(6307..6834)
                     /locus_tag="VMZ53_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27712.1"
                     /translation="MTRLASLLCVITAACGGGTATGEASLSNTMPPVKSAAAKPFTGA
                     DGAGAMVLGWKIELYSDDAGASCTEASKVGTIGIYSNQAAGSGPQALLSTGFITIVTA
                     SPPTVLATAAANLSVEGVSIMSGGVELTEFHLTADAKHADRLAGTINAGGTDAGTGAD
                     VTLTGMFTAPICEEE"
     gene            6909..8153
                     /locus_tag="VMZ53_04370"
     CDS             6909..8153
                     /locus_tag="VMZ53_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HUS27713.1"
                     /translation="MRLLHTSDWHLGHTLKEVTREYEHQAFLAWLLETCVRETPDVLV
                     ITGDVFDSATPPASAERMWFELLAAARRASPAMDIIAIAGNHDSPARLAAASSVLREL
                     GVHVLGSLPHCADGSIDMDRVLIPVAGGRGLVAAVPFLRPLDLGSAEDPLAAVYADVI
                     GAARARLAADQAFIVLGHLYAAGAEPSASERRVSIGGQESASLRMFPREIDYVALGHI
                     HRSQRIGRETIRYAGAPIALSIDEASYRHQVVVVDFDGAKPAGQRAAQIRGIEIPQVI
                     EIVRVPKRGALPLTEVLAALAELPERRKGDDPECPYLEVDVLLERPEPRLRATLENAI
                     EGKRARLISIQTKRTGDGAALGDRKIATRLAELDPRDVFGQLWARDHAEPPSAAVVGA
                     FERLLAEVTGGLDDPERQRHAS"
     gene            8150..>11760
                     /locus_tag="VMZ53_04375"
     CDS             8150..>11760
                     /locus_tag="VMZ53_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS27714.1"
                     /translation="MRILAIRGCNLASLAGEFEVDLSKGALAEAGVFAIVGPTGAGKS
                     TLLDAMCVALFDRTPRLANRSTVVVGRGDDDPTALGAQDVRSLLRRGASAGWAEVDFE
                     SGDLRRYRARWSVRRARGSTEGRFQNQQVTLTAIDPEGRSESALHLGGGDQLGGTKTE
                     TLAAIQHRLGLSFDQFRRSALLAQGEFAAFLRADGKDRSELLERMTGTEIYSRLSVAA
                     HQKATLAVQQLRDRQGVMAAIPVLSDEARMQAEADLVLAKQAQEASRVRLAAAQAAAR
                     WLTEADRRQRAVAEAETEHAARDLAVKSAEVLRAEHAVRRRAESMRPAWDAAAAIDRQ
                     VAIANGELATAATTLASAETAQRDSAARHTRLSELHAPIREARVAAGLVEKKVAKKTP
                     RTRDAAADAKADANAGARTDARADAARAARDPQLEARALAAASDAEWLASRHELASAI
                     AAWRELDGKLGAVATLATEIAACDRALDDHARKRDVLAAQHGAATTEHQQAIARLADA
                     RREAASYQQKRGLSLEAARRQEDEARRRSAELDKLVAITGEARGAATARAELERQLAQ
                     LAATANADGEHRRTAEADRDAAALLRDERARHVEELRKAAGYAHARAELVDGDPCPLC
                     GAEEHPWADRGSFDALIVDAQTRLAEAIARGDAARSILATLAAREQHRATERTRLTSC
                     LNTADATAQAAHARWRTQLASLGELLLVDDPSTPAAQQLADERIEGARTKLEAARAAR
                     SHAEAAANAANEAQARVQARHADVEHHALRLRELEASLGQLDAASERVRGERTGKLER
                     DAELRAQIAASLALWTSSGLAQSETTLIAGTLTALKRTFADAAPDADARADARANVLD
                     LQPCRQLLSTIAAEWAKRADAIIEIDALLAAALIASEELVHEHERDVVAAQARVTACT
                     TRREALRIELAEAADRLEAARVTAGFAREELQRLLSADAARVDELAAQLAALDRAADR
                     ARTLVIERRRLVEDHEASRPNFADPAVQARALEDAQDPASLKTLAAAVDAADGHAASL
                     AASLAADADARARRAAAAAELAAAEQAAEVDRVLGEVIGSHDGKAFRSFAQSLTLDAL
                     LAVANSHLEELAPRYQLERVPKHDLELQVIDRDLGDEIRSVQSLSGGESFLVSLALAL
                     GLSSMSAHDVRVRTLLIDEGFGTLDPATLDTALAVLDELQSTGRQVGVISHVPALVER
                     "
BASE COUNT         1952 a         4045 c         3919 g         1844 t
ORIGIN      
        1 cgctttgcgc tctttcgtgt acgcgatcgt gtcttgcagc acctgctcca tcgacgcgac
       61 tgcaccgatc gcgaccgcga gccgctcttg ctgcagcttc tgcatcatca tcatgaagcc
      121 gccgccctcc acaccgagga gatttgtcgc cggcacgcgg cagtcttcga agaacagctc
      181 gctcgtgtcc tgcgacgcca tgcccatctt gtggaggcgc ttgcccttct ggaagccctt
      241 gcggttcgcc tcgacgacga atagcgagat gccgcgatgc gcgttctcgg gattcggatc
      301 ggtcttcgcg gcgacgacgc agatatcgca gagctggccg ttcgagatga acgtcttgct
      361 gccgttgatg acgtactcgt tgccgtcttt gaccgcggtc gtcgcgagcg cggcgaggtc
      421 cgagccggtg ccgggctctg tcatgccgag cgcagtgacg atctcgccag acgcgcagcc
      481 gggcaaccac ttctgctttt gctcttcgct gccgaacgaa tagatgtacg gcacgatgat
      541 gtccgagtgc aggcccatcg cgaagccgct ctcgtagacg cgcgcgagct cctccatgat
      601 gatcgtcgcg tggaggaaat cgccgccgga gccgccgtac ttctcctcca tccacgtgca
      661 gaggaagcct tgcgctcccg ccttgcgcca gatctcgcga tcgacctggc cttgctcgcg
      721 ccacttctgt tggttcggaa cgatctctcg gtccgcgaat tggcggaacg ctttgcggaa
      781 ggctaggtgc tcttcggaga acagagtgcg gtccattcag cgttcgtaac acgctgccga
      841 tgtgtgcgtg cacgtctcca aggctccgac accgagatgc tgcttttcgg tttgcgcgag
      901 cgatcgcagt cttctaccct caggggatgg gtaacggtga tcaccgaaag ccggagagcg
      961 ctaccccgga cctaacggtc gggcggacga cgacgcttga cgacccgctg acgaccggcc
     1021 tcctcgccga agtcgcgcgc agcgcgcaga cgaaggaatt cgacggcgac gcgattcaac
     1081 agctgatcga acagatgcag gacgacgaca ccgccgtcat catcgacaag cccgtcgcgc
     1141 atccgaacgt gcgcaagcgc ggctaagcga gcgcgaggtc agcgcacttc tcggcgatca
     1201 tgatcgtcgg cgcgttcgta ttgccgccga tgattttcgg catcacggac gcatcggcga
     1261 cgcgcagatt gcgcagacca tgcacgcgca gatcgggacc gacgacggcg tccggccccg
     1321 tgcccatctt gcaggtgccg accgggtgga agatcgtgtt gcagcccgcg cggatgttgt
     1381 cgcgcagctc gtcgtcgctg gcgacgcccg ggccgggata gatctccttg ccgaggatct
     1441 gtttgagcgc gccggtcgac gcgatctcgc gcgcctgttt cacgccggtg acgagcagct
     1501 cgaggtcagc tgcgtcgctg aagtacttcg gatcgatcgc gggcggagcg gcaggatcgg
     1561 cgctcgcgag acgcagctcg ccgacgctct ttggatagat gaggccgggc aacaggcacg
     1621 cgaaccggcc gaacgacggg ctcacaaggc cgtggtcgct cggcagcttc acgccgaacg
     1681 gagcgacgtg aaactggatg tcgggcagcg gcgcgctcgt gctcgaccgt acgaacgcac
     1741 cggcgtcgag cgcagcgccg gccagcggcc caccgctagt cgccgcgaac cgcgcgatcc
     1801 accacagcat ggctgccttc gacagctttg gcgacgacgc cgcttcgaag ttcaccgcat
     1861 gcagcaggtg atcctgcagg ttcttgccga cgcccgcgag ctcgtgcttc ggctcgacgc
     1921 ccgctgcctt cagctcgctc gcagggccga tgcccgacaa cagcagcaag tgcggcgacc
     1981 caatcgcgcc gccggcgagg atgatctcct tgccttcgat cgtctgctcg cctttggtcg
     2041 tgcggatgcg aacgccggta acgcgatcgc ggtcgagcac gaggccgagc gcatgcgcac
     2101 cgctcagcac cgtcaggttc tcgcgcggca gcgccggctc gagaaacgcg atcgccgtgc
     2161 tcgcgcgatg tcccttcttg atcgtcgact gatagtgacc cgcgccttct tgatcggcgc
     2221 cgttgaagtc gtcgttgacg gcaaccttgc agcgcgccgc gatcgcttcg acgaaggcct
     2281 gcgcgaagcc cgatggctcg cggttgtcgc tgacgtgcaa cgggccgcct gcaccgtggt
     2341 actcggacgc gccgcgcacg ttgtcctcgg atttcttgaa gtacggcagc acgtccgcgt
     2401 acgaccagcc cggtaccgcc cagtcgtcgt agttctggcg attgccgcgg atgtagacca
     2461 tcgcgttgag gcagctcgtc ccgccgagca ccttgccgcg cggccagaag tgcgaccgct
     2521 tcgcgcagtg ttcttgcggc acggtcttga acgcccaatc gagcggcgta ctccacagca
     2581 gctgatacag ccgcggcgct cggaccttga gcgacttgtg ctgcgcgccg cccgcctcga
     2641 tgagcgcgac cttgcgcccg ccgacgctga gccgattcgc gagcacgcaa cccgccgacc
     2701 ccgcacccac gatcacgaaa tcgaacatgc ggaccatgct atgccagccg ggtcgtggcc
     2761 gataccgcat ggacggtcga acaagcaggc gtcaccgtgg ctgccttcgt caaggcgaag
     2821 ctcggcgtgc cgtggaccgt tgcgaagcgt cacgtctcga cgggcaaggt ctccgtcgac
     2881 ggcgccgtcg tcaccgagat cgatcatcgg ctcgcggccg gccagcgcgt cgcgctccag
     2941 atgtcggcgc ccaaaccgcg cgatcccgag cgcgaaggtg acctcgtcta cgacgacgcg
     3001 cacatcgtcg tgatcgacaa gcccgccggc gtgaacagcg tgccgtacga agagcgcgag
     3061 agcggcaccg cgatggactt gatccgcggc gcgtggcgac gcatgggcaa gccagcgacg
     3121 agcgtcgcgc ttcatgtcgt gcatcgcatc gatcgcgcga cgtcggggct gctcgcattt
     3181 gccaagacga agcgcgcgga aatcggcatg gcagcacagc tgcgcgcgca cacgatggag
     3241 cgcatgtaca tctgcgtgac gcacggcgtg atgccggcgg gtcgcatcga atcgcagctc
     3301 gtcgccgatc gcggtgatgg cctgcgcggc tcgacgtcgg atccgacgcg tggcaagcgc
     3361 gcgatcacgc atgtcaccgt ccgcgaatcg ctgcgcaaag cgacgctctg cgaagtgcgg
     3421 ctcgagaccg gcaagacgca tcagatccgc atccacctcg cggagtcggg gcatccgctc
     3481 gtcggcgaaa cggtctacat ccgcgactat cgcggcacgc cgatcgactc gccccgcctg
     3541 ttgctccacg ctgcgacgct cggcttcatc catccgatca cgaacgagca cgtatcgctc
     3601 agctcgccgt tgccgcctga cttcaccgcg gtcgttgatc ggttacgctg aacagcatgg
     3661 tcgaggacct catcattcgc ttcgccgtgc cgaacgacac cggctcgatc ctgtcgttca
     3721 ttcgcgacct cgcggagtac gagcgcctac tgcatgaagt cgtcgccgac gaggaaacgc
     3781 tgcgcgcgac gctgtttggc gtgaagcccg cggccgaggt cttgatcgcc gagcgcgccg
     3841 gaacgccggt cgggttcgcg ttgttcttcc cgagctactc gacgtttctc gcgaagcccg
     3901 gcctgtatct cgaggatctg ttcgtgcgcc ccgccgagcg cggcaaaggc gtcggtggtg
     3961 cactgatggc ggcgctcgct cgcatcgccg tgcagcgcaa ctacggccgc ttcgagtgga
     4021 gcgtcctcga ttggaacgag cccgcgctga agttctatgc ctcgctcggc gcgaagccgc
     4081 agtccgagtg gacggtccag cgcctcacgg gagacgcgct ctacgcgctc gcggacagct
     4141 ggccaacgcc cgtcggcaag acctgatccg cggcatcctt ctgctgatac cactgtcgct
     4201 gcgagcgaaa cgcgatgttc gctgcgtcga tgatcgcgga tggtgactgc gtgtgcgcga
     4261 gctcgccgag gcgcgtaccg aagtcacgca tacgcgcgcc aggcgctccg ccgtacgcgt
     4321 tgaggtacat cgtcggcgtg ttcgtgccga gcacctgctg aatctgtcgg cgcagcagac
     4381 cggcgagcag agtctggcgc tcgaggacaa acatccagcc gagcgcttcg ggcgccgtac
     4441 gaatcgtcac ggtcgcgcgc gggcactcgc cgatgtcacg ttccgacatg ccaagcgcga
     4501 gcagatcgcg acgcaagagc ggcacgcggg ctcgctcacg cacgaatcgc agctcgatct
     4561 ggtgcacttt cgcgacggct tcttcgacag gcgcttcgaa gccgagcacg cgaacgagaa
     4621 acgatcggta ctcgtggatc gacgagatgg tcagtgctgc gaggcgatct tcgtcggcag
     4681 gcgcgtgatg atgcgtcgtc tcgaggccga gtcggacgag catccatgga gggctcaagg
     4741 gaacctcagt tgttcggtgt ctacggaggc gctagaggtc gcgggcaaga gcacccactc
     4801 ctcgaggcat tgcagcgcag cgtgaaccgc gaggagcgtg gcgtcgagct cgggttgcgc
     4861 gtgaacgtgg cgatcgatga cacgtccgag ctcgtcccag ctcgtttcga tctcgatctc
     4921 cggtgtgccg agaaattgtc cggcgacctc gagctcgctt ggcaacgcga ggcatagccg
     4981 tcggcgaatc gctggcagct cgcgcatcat tctctcgacg acgaaaagcc agccgagcgc
     5041 tttgtaagcc gtatcgaacc gcggcacgac gcaacgctgc gagaggcgca cgcgttcata
     5101 cggcgacagg ccgagcgaca gtagatcgct cgccatgcgg cccgacttga tgcgctcttg
     5161 aatgaagcgc aggtcgagcg tgtacgcgta cgcgagccgc gcttcgaatg cgacgatgaa
     5221 gccgtagagg tcgcacaagt accggcgata cgaagcgcgc gtcggctgaa tcagtggtgc
     5281 cgccacgatc gaggcaacgg tctccgcgcg agcgccatac cgcttctcga cgcgagtcag
     5341 tgcccagctc gacttcatcc gccgtgctgg gtgaccgccg gtgccgcgcg cgatcgcttc
     5401 gtcgtggact tcgctcatgt catcgccacc ggacgggcac gcttggagtg agtgcttcgt
     5461 gctcgccgtc gtgacccttg tgacgcgcac agcgtagggg gccacgctcg accgcgcaca
     5521 gctccgtggt ttgacgctcg tcgagccgag gcttggtcgt ctgtggttgg ttcgagctca
     5581 tcccgttctc ctggcgtcag cagacatgcg gaaccagcta tgcgcggctt cgaaggcctc
     5641 gcacgcagcg cgttcgatct cggcagcagc gcccggctcg accgtggcgt cgtcgatgac
     5701 gcggccgaac cgggaccaat aggacatgtc gttcgagaac gcctcgaggt agcgcgtcgc
     5761 gttcacgacc tggggcaacg agctgcgcac gtgccgcaaa aggccatcgg cgaggagcgt
     5821 cgttcgctcg accacgtaga gccaaccgag tgcctcgcgt gtcgtcgcga acatcgtgat
     5881 ctccgggcat gtcgcgatgg acgcgacttg cgacggcgtc aggtcgagct cgagcaggtc
     5941 ctgcgcgatg aggccggcgc gaccaacgcg ccgcagatcg acgacaacgc gcgcgaggcc
     6001 cggtgtgtac ttacatgcgc tctcgaacgg tgcgaccagg ccgtaggtac ggacgagctg
     6061 ggcgaggtaa tccgtgacgg tgaccgtcgg ccggaggagc tccagccacg aatcatcgac
     6121 ggccgaatgc cactgccttg tcgccgcgtt cagcctgacc aatgcggtgc tgacgtcctt
     6181 caccttgagc gggacgcgta agaagcaaaa cgcgttcaca acccggcgcg tgccgagtcg
     6241 ggcacatagg tcgggccttc cgtgcggcgg cgtcgcgtag agcctggccg cacgtagcgg
     6301 agtggctcac tcctcttcgc agatcggagc cgtgaacatg cctgtaagtg tgacgtctgc
     6361 accggtgcca gcgtcggtcc caccggcgtt gatcgtgccg gcgaggcgat cggcgtgctt
     6421 cgcgtcggcc gtgaggtgga actcggtcag ctccacgccg ccgctcatga tcgacacgcc
     6481 ctcgacactg aggtttgccg cggcagtcgc gagcacggtt ggtggcgatg ccgtcacgat
     6541 cgtgatgaag cccgtcgaca gcagcgcctg tggaccggag ccggcggctt gattggagta
     6601 gatcccaatc gtgccgacct tactcgcctc ggtgcatgac gcgcctgcgt catccgagta
     6661 gagctcgatc ttccaaccga gcaccatcgc gcctgcgcca tcggcgcccg tgaatggttt
     6721 ggccgcggcc gatttcacgg gtggcatcgt gttggacaac gacgcttcgc ccgtggcggt
     6781 tccgcctccg caggcggcgg tgatcacgca tagaagggac gcgagcctgg tcatggcgcc
     6841 gaacctatcc ctgccaaggg ggcaggcgca aatgaggatc agacccctgt gcaaatgtcc
     6901 catgctcgat gaggctgctc cacacgtccg attggcacct cggacacacg ttgaaggagg
     6961 tcactcgtga atacgagcac caggccttcc tcgcgtggct gctggagacc tgcgtccgcg
     7021 aaacgcccga tgtcctggtg atcacgggcg acgtcttcga tagcgcgacc cctccggcga
     7081 gtgccgagcg gatgtggttc gagctcctcg cggcggctcg acgcgcctcc ccggcgatgg
     7141 acatcatcgc gatcgcgggc aatcacgatt caccggcgcg cctggcggcg gcgtcgtcgg
     7201 tgctgcgcga gcttggcgtc cacgtgctcg gcagcctgcc ccactgcgcc gacggctcga
     7261 tcgatatgga tcgcgtgctg atcccggtcg cgggcggccg cggcttggtc gcggcagtgc
     7321 cgtttctgcg gccactggac ctcggcagtg ccgaagatcc gctcgccgca gtctatgcag
     7381 acgtgatcgg cgccgcgcgc gcaaggctcg cagcggatca ggcgttcatc gtgctcggtc
     7441 atctatatgc agcgggcgcc gagccatccg cgagtgagcg acgcgtgtcg atcggcgggc
     7501 aggaatctgc gtcgctgcga atgttcccgc gcgagatcga ttacgtcgcg ctcggccaca
     7561 ttcatcgctc gcagcggatc ggacgcgaga cgattcgcta cgcaggtgcg ccgatcgcgc
     7621 tgtcgatcga tgaggcgagc tatcggcatc aggtcgtcgt cgtcgacttc gacggagcaa
     7681 agccggctgg gcagcgcgcg gcgcagattc gaggcatcga gatcccgcag gtgatcgaga
     7741 tcgtgcgcgt gccgaaacga ggcgcgctgc cgctcaccga ggtgctcgct gccctcgcgg
     7801 agctgcccga gcgccgcaag ggcgatgatc ccgagtgccc ctatctcgag gtcgacgtgt
     7861 tgctcgagag gccagagccg cgattgcgcg cgacgctcga aaacgcgatc gagggcaagc
     7921 gcgcgcgtct catcagcatc cagacaaaac gaacgggcga tggagcggcg ctcggtgatc
     7981 gcaagatcgc cacgcggctc gccgagctcg atccccgcga tgtgttcgga cagctgtggg
     8041 cgcgcgatca cgcggagccg ccgagcgctg ccgtcgtcgg tgcgttcgag cggctgctcg
     8101 ccgaggtcac gggcggcctc gacgatcccg agcggcagag gcacgcgtca tgaggatcct
     8161 cgcgatccgc ggctgcaacc tcgcgtcgct cgcaggcgag ttcgaagtcg atctgtcgaa
     8221 aggcgcgctc gccgaagcgg gtgtgttcgc gatcgtcggc ccgacgggcg caggcaagag
     8281 cacgctgctc gatgcgatgt gcgtcgcgct gttcgatcgc acgccgcgcc tcgccaatcg
     8341 cagcacggtc gtcgtcggtc gcggcgatga tgatccgacg gcgctcggtg cgcaggacgt
     8401 gcgcagcctg ctgcggcgcg gtgcgagcgc gggctgggcc gaagtcgatt tcgaatccgg
     8461 cgatctgcgc cgctatcgcg cgcggtggtc ggtgcggcgt gcgcgcggct cgacggaagg
     8521 ccgattccaa aaccagcagg tcacgctgac cgcgatcgat cccgagggtc ggagtgaatc
     8581 cgctctccac ctcgggggcg gcgaccagct cggcggcacg aagacggaga cgctcgcagc
     8641 gatccagcac cggctgggct tgtcgttcga tcagttccgg cgctctgcgc tcctcgcgca
     8701 aggcgagttc gccgcgttcc tgcgcgccga cggcaaagat cgcagcgagc tgctcgagcg
     8761 catgacaggc accgagatct actcgcggct gtcggttgcc gcgcatcaga aggcgacgct
     8821 ggccgtgcag cagctccgcg atcggcaagg cgtgatggcc gcgatccccg tgctgtccga
     8881 cgaagcgcgc atgcaagcgg aagccgatct cgtgctcgcg aagcaggcgc aagaagcgtc
     8941 gcgcgtgagg ctcgccgctg cacaagccgc ggctcgctgg ctgaccgagg cggaccgcag
     9001 gcagcgcgcc gttgcggagg cggagacgga gcatgcggcc cgcgacctcg cggtgaagag
     9061 cgccgaggtg ttgcgtgcag agcacgccgt gcggcgtcga gcggagtcga tgcggcccgc
     9121 gtgggacgcc gcagcggcga tcgatcgcca ggtcgcgatc gcgaatgggg agctcgcaac
     9181 ggcagccacg acgctcgcgt ccgcagagac ggcgcaacgc gacagcgccg cccggcacac
     9241 gcgcctctcc gagctgcacg cgccgattcg cgaagcgcgc gtcgcggcgg gcctcgtcga
     9301 gaagaaggtc gcgaagaaaa cgccgcgcac gcgcgatgcg gcagccgatg cgaaggccga
     9361 cgcgaacgcc ggcgcgagga ccgatgcgag ggccgatgcc gcgcgtgccg cgcgcgaccc
     9421 gcagctcgaa gcacgtgcac tcgctgcagc gagcgatgcg gagtggctcg cgtcgcgtca
     9481 cgagctcgct tccgcgatcg cggcgtggcg cgagctcgat ggcaagctcg gcgcagtcgc
     9541 cacactcgcg accgagatcg cggcgtgcga tcgtgcgctc gacgatcatg cgcgcaagcg
     9601 cgacgtgctc gccgcgcaac acggtgccgc gacgacggag caccagcagg cgatcgcccg
     9661 cctcgccgac gcgcgccgcg aggccgcgag ctatcagcag aagcggggcc tctcgctcga
     9721 agccgctcgc cgccaagaag acgaggcgcg ccgccgaagc gcggagctcg acaagctcgt
     9781 cgcgatcacg ggcgaagcac gcggcgcggc caccgcgcgc gcagagctcg aacgccagct
     9841 cgcacagctc gccgcgacgg cgaacgccga tggcgagcac cgtcgcaccg ccgaagccga
     9901 tcgcgatgcc gccgcgctcc tgcgcgacga gcgcgcgcgc cacgtcgaag agctccgcaa
     9961 ggccgccggg tacgcacacg cacgcgccga gctcgtcgac ggcgatccgt gcccgctgtg
    10021 cggcgccgaa gagcatccat gggccgatcg tggctcgttc gacgcgctca tcgtcgacgc
    10081 gcaaacgcgc ctcgccgaag cgatcgcgcg cggcgacgca gctcggtcga tcctcgccac
    10141 actcgccgcg cgcgagcagc accgcgcgac cgaacgcacg cgcctcacct cgtgcctcaa
    10201 cacggccgac gcgacggcgc aggccgcgca cgctcgctgg cgcacgcagc tcgcatcgct
    10261 gggcgagctc ctgctcgtcg acgatccgtc cacgcccgcc gcccaacagc tcgccgacga
    10321 acgcatcgaa ggcgcgcgga ccaagctcga agccgcacgc gccgcccgca gccatgccga
    10381 agcggccgcc aacgccgcga acgaagcgca agctcgcgtg caagcgcggc acgccgacgt
    10441 cgagcatcac gcgctgcgcc tgcgcgagct cgaagcgtcg ctcggccaac tcgatgcagc
    10501 gagcgaacgc gtccgcggcg aacgcacggg caagctcgag cgcgatgccg agctgcgcgc
    10561 acagatcgcc gcgtcgctcg cactctggac tagcagtggc ctcgcgcaat ccgagacgac
    10621 gctcatcgcc ggaacgctca ccgcgctgaa gcgaacgttc gccgacgctg cccccgacgc
    10681 cgatgcacgc gccgacgccc gcgcgaacgt gctcgatctc cagccgtgcc gccaactgct
    10741 ctcgacgatc gccgccgagt gggcaaagcg cgccgacgcg atcatcgaga tcgatgcgct
    10801 gctcgccgcg gccctcatcg cgagcgaaga gctcgtgcac gagcacgaac gcgatgtcgt
    10861 agcagcgcag gcgcgcgtca cggcgtgcac gacgcgtcgc gaggcgctgc gcatcgagct
    10921 ggcggaagcc gccgatcgtc tcgaagccgc acgcgtcacc gcgggcttcg cacgcgaaga
    10981 gctgcagcgc ttgttgtccg ccgatgccgc gcgtgtcgac gagctcgccg cccagctcgc
    11041 cgctctcgac cgcgccgcag atcgcgcacg cacgctcgtc atcgaacgcc gccgcctcgt
    11101 cgaagatcac gaggcctcgc gcccgaactt tgctgacccc gcggtgcaag cgcgcgccct
    11161 ggaagacgcc caggatccag cctcgctgaa gacgctggcc gccgccgtcg acgcagccga
    11221 tggtcacgct gcgtcgctcg ccgcgtcgct cgccgccgac gccgatgctc gcgcccgccg
    11281 cgccgcggcc gcagccgagc tcgccgcggc cgagcaagcc gccgaagtcg accgcgtcct
    11341 cggcgaagtg atcggctcgc acgacggcaa ggcgttccgc agcttcgcgc aatcgctcac
    11401 gctcgacgcc ctgctcgccg tcgcgaactc gcacctcgaa gaactcgcgc cgcgctacca
    11461 actcgagcgc gtcccgaagc acgacctcga gctgcaagtc atcgaccgcg acctcggcga
    11521 cgaaatccgt tcggtccaat cgctgtccgg cggcgaatca ttcctcgtct cgctcgccct
    11581 cgccctcggc ctctcctcga tgtcggcgca tgacgttcgc gtccgcacgc tcctcatcga
    11641 cgaaggcttc ggcacgctcg accccgcaac gctcgatacc gcgctcgccg tcctcgacga
    11701 gctgcagtcc actggccgcc aggtcggagt gatctcgcac gtccccgcgc tcgtcgaacg
//
LOCUS       DATLBZ010000081         2880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3016149, whole genome shotgun sequence.
ACCESSION   DATLBZ010000081 DATLBZ010000000
VERSION     DATLBZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3016149"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..424
                     /locus_tag="VMZ53_04380"
     CDS             101..424
                     /locus_tag="VMZ53_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27715.1"
                     /translation="MYTLGALATLDRPRKLGSFVLSPGIGVGYTYLHVTTTHRDAMMN
                     PFDIPTTDHQLRGGAHAALLKSLSDHIGAFADLWVDAAALRSDSQFGPSAYLSFALGL
                     RVEER"
     gene            421..1011
                     /locus_tag="VMZ53_04385"
     CDS             421..1011
                     /locus_tag="VMZ53_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS27716.1"
                     /translation="MSEGKLLPFRRVEGRTEELSDRALVSAIAEGDQAALGALYDRFQ
                     RDIYRFVARLASASDADDFVQMAFLEAYRSAPRFRGDSAVKTWLFGIAANLVRNHHRG
                     TQRKQRAHDSLQTMPRRPPTDPGEAITREQQRQWLARAIESLSPALREAYVLCVIEEV
                     PGKEAAQALGVREASLYRRLTDARNAIRAAIEEMQS"
     gene            1008..2426
                     /locus_tag="VMZ53_04390"
     CDS             1008..2426
                     /locus_tag="VMZ53_04390"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HUS27717.1"
                     /translation="MTAKWIPADDELDAIARSMPVAERGADRIEQERTTLLAQAAGAS
                     QQRRGSRTPIIASAAVAFAAAAAVMLWLVVRPSGQTHARESITALGPAQYERVATWPD
                     FKARLDEGRIEVHVSKLGGGDRFRIVTADAEVEVRGTQFVVGADHGSIASVAVTEGRV
                     EVRWMHEQPVFLSAGQTWRPTRTAQRDEVQLTPPVQQDGSSQQEAATTHIDTKAPVGT
                     EVSSTIEKATSATTNQRVATNTGSKTTKSAASDPNSAETKTIDTKVAGASPNETKPTT
                     DAKPQTIATKQDTPKPQDTSKPQGTSKPQDTSKPQDTSKPQDTSKPQDTSKPQATSKP
                     QNTPKPQPLAPGEAEFRAGIASLRAGDASAAVTSFASACKSAAKEALGEDACFWLGAA
                     AKRAGQTATARQALTRFLETYPSSARAGEASALLGWLLYDAGELDAAQHRFELAARDR
                     VPKVKESAERGLEAIKRKRGAP"
     gene            complement(2472..>2880)
                     /locus_tag="VMZ53_04395"
     CDS             complement(2472..>2880)
                     /locus_tag="VMZ53_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27718.1"
                     /translation="FSRMPVGGSFVANVLVPSLLGAIGMSLAYIPAMITSTMGARPED
                     GGLASGLMNTTYQVGSALGLAAMVAVASAKTTALATSGAERLSSLNGGFSSAFIGAAV
                     IAGAAALLSAVAIRMPAKQASAAPQDGAAGSHA"
BASE COUNT          542 a          965 c          964 g          409 t
ORIGIN      
        1 ctccgcgtgc atgcggatcg gttcgctgtg cgcgggggcg accttgcgct cgcaactgga
       61 cacgcgggcg agcggcgaca ccacggcgat caatcaaagc ttgtacacgc tcggagcgct
      121 cgcgacgctc gatcgtcctc gcaagctcgg cagctttgtc ctgagcccgg gcatcggagt
      181 cggctacacg tacctgcacg tcacgacgac gcatcgcgat gcgatgatga atccgttcga
      241 cattccgacg acggatcatc agctgcgcgg tggcgcgcat gcggcgctgc tgaagtcgtt
      301 gagcgaccac atcggcgcgt tcgcggatct gtgggtggat gcggcggcgc tgcggagcga
      361 ttcgcagttc ggtccgtcgg cgtacctctc gttcgcgctg ggactgcgcg tcgaggagcg
      421 gtgagcgaag gcaagctgct tccattccgt cgcgtcgagg gacgcacgga ggagctgtcc
      481 gatcgcgcgc tcgtgtcggc gatcgccgag ggcgatcagg cggcgcttgg cgcgctctac
      541 gatcgctttc agcgcgacat ctatcggttc gtcgcgcggc tggcgtcggc gtccgatgcg
      601 gatgacttcg tccagatggc gtttctcgag gcataccgat ccgcgccgcg cttccgtggc
      661 gactccgcgg tgaagacatg gctgttcggt atcgcagcga acctcgtgcg caatcaccat
      721 cgcggcacgc agcgcaaaca gcgtgcgcat gactcgcttc agaccatgcc gcgccgtccg
      781 ccgacggatc ctggcgaggc gatcacgcgc gagcagcagc gccagtggct cgcgcgcgcg
      841 atcgaatcgc tgagcccagc gctccgcgaa gcgtacgtgc tctgcgtgat cgaggaagta
      901 cccggcaagg aagccgcgca ggcgctcggc gtgcgcgaag cgagcctcta tcgtcgtctc
      961 accgacgcgc gtaacgcgat ccgcgctgcg atcgaggaga tgcagtcgtg accgcgaagt
     1021 ggattcccgc ggacgacgag ctcgacgcaa tcgcgcgttc gatgcctgtt gccgagcgcg
     1081 gcgccgatcg catcgagcag gagcgcacga ctctgctcgc gcaagcggct ggcgcgtcac
     1141 agcagcgtcg aggatcgcgc acgccgatca tcgcgagtgc ggcggtggcg ttcgcggcgg
     1201 cggctgccgt gatgctgtgg ctcgtcgttc ggccgagcgg ccaaacgcat gcgcgtgagt
     1261 cgattaccgc actcggtcct gcgcaatacg aacgcgtcgc aacgtggcct gacttcaaag
     1321 ctcgactcga tgagggtcgt atcgaggtcc acgtgtcgaa gcttgggggc ggcgaccgct
     1381 tccgcatcgt cacggccgat gccgaagtcg aagttcgtgg cacgcagttc gtcgtcggcg
     1441 ccgaccacgg ctcgatcgcg tcggtggctg tcaccgaagg tcgcgtcgag gttcgctgga
     1501 tgcacgagca gcccgtcttc ctgtcggcag gtcagacgtg gaggccgacg cgaaccgcgc
     1561 aacgcgacga ggtgcagctc acgccgccgg tacagcaaga cggctcgagc cagcaagagg
     1621 cggcaacgac ccacatcgac acaaaggctc ctgtcggaac cgaggtgtcg tcgacgatcg
     1681 agaaggcgac gagcgcaacg acgaaccagc gcgtcgcgac gaataccggc agcaagacga
     1741 ccaaatcggc ggccagcgat ccgaactcgg ccgagacgaa aacgatcgac acgaaggtgg
     1801 ccggcgcctc gccgaacgag acgaaaccaa cgacggacgc gaagccgcag accatcgcca
     1861 cgaagcagga cacgccgaag ccgcaagaca cgtcgaagcc gcaaggcacc tcgaaaccgc
     1921 aagacacgtc gaagccgcaa gacacgtcga aaccgcaaga cacgtcgaaa ccgcaagaca
     1981 cgtcgaaacc gcaagccacg tcgaaaccgc aaaacacgcc gaagccgcag ccgctcgcac
     2041 caggcgaagc cgagttccgc gcgggcatcg cttcgctgcg cgcaggcgat gcgagcgccg
     2101 cggttacgtc gttcgcgtcg gcgtgcaagt ccgcagcgaa ggaagcgctg ggcgaggacg
     2161 catgcttctg gctcggcgct gccgccaagc gcgcgggaca gaccgcgacg gctcgtcaag
     2221 cactcacgcg gtttctcgag acgtacccga gctcggcgcg cgccggcgaa gcctctgccc
     2281 tcctcggctg gttgctctac gacgcaggcg agctcgacgc tgcacaacat cggttcgagc
     2341 tcgccgcgcg cgatcgcgtg cccaaggtaa aggagagcgc cgagcgcggc ctcgaagcca
     2401 tcaagcgcaa gcgcggtgcg ccctgagttt ggctagagcg acggcctcga ctacgaaggc
     2461 cgaaccacga tctacgcgtg cgaacccgcg gcaccgtcct gcggcgctgc gctcgcctgc
     2521 ttggccggca tgcggatcgc gaccgccgac agcagcgcag ccgcgcctgc aatgacggca
     2581 gcgccgatga acgctgacga gaatccgccg ttcagtgacg acaagcgctc ggctcccgat
     2641 gtcgcgaggg ctgtcgtctt cgcactcgcc accgcgacca ttgccgcgag gccgagcgcc
     2701 gaccccactt ggtacgtcgt gttcatcaga cccgacgcca ggccaccgtc ctcgggacgc
     2761 gcgcccatcg tcgacgtgat catcgccggg atgtaggcaa gcgacatgcc gatggcaccg
     2821 agcagcgacg gcacgaggac gttcgcaaca aaggaacccc cgaccggcat gcgcgagaac
//
LOCUS       DATLBZ010000082         5900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6742337, whole genome shotgun sequence.
ACCESSION   DATLBZ010000082 DATLBZ010000000
VERSION     DATLBZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6742337"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1387
                     /locus_tag="VMZ53_04400"
     CDS             110..1387
                     /locus_tag="VMZ53_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HUS27719.1"
                     /translation="MTALFTAGALAKAWLATLLMMAAQGTLLALLAFVLARAGKLRPA
                     WQAAIWLVVTVKLALPWGPSMPYSLADAIAMFRDHPAPAPMLHIAPSAPVVATTPDAW
                     PAVGWLLLAALWLLGATWVVVRAVLAHRSTTAAARRAPLAPRDALALIAQLSAGLRVR
                     TPKLSVGDADVGPHVVGLVRTIIVVPPSLLGDEPLLRAALLHELAHVRRRDAVARVIQ
                     IIAASIMWWWPVARIVQRRLEAAREAACDAWALETVDVPRAAYARLLVRMASLRHAAA
                     PGLAAHHTPSSLNKRVAAVLGPPARARLSWLHRIALAAWVILALGGARSATAHTHTEA
                     CHYTPQLGMALYNSYPQADLDGDGMLSRTEACDLQAELMRDRRDVSSRLTPEAEREME
                     TLLAEPLCCNLPTTEVYSSQDSDTCRNVIGVEP"
     gene            1384..1653
                     /locus_tag="VMZ53_04405"
     CDS             1384..1653
                     /locus_tag="VMZ53_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27720.1"
                     /translation="MKVLSVLLAVLALAACQNDTKNLNDKIDKMDKKLDAILAQRGGG
                     GGAAPQRPSRPEPDRSKTYAVSVDGDPFEGPADAKVTIVEAFDYA"
     gene            1666..2205
                     /locus_tag="VMZ53_04410"
                     /pseudo
     CDS             1666..2205
                     /locus_tag="VMZ53_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            2320..3144
                     /locus_tag="VMZ53_04415"
     CDS             2320..3144
                     /locus_tag="VMZ53_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUS27721.1"
                     /translation="MKTVPILLAALALAACQNDTKNLNDKIDKLDKKLDAVLAQRGGG
                     NPGAAQQRPQRPEPDRSKTYSVSVAGDNFDGPADAKITVVKAYDYACPYCERVRSTMD
                     ELHKKYGEDIRVVYKQLVVHPQTAMTGALAFCAAGKQGKATQMDALIWDKGFKARKFD
                     ADQCWTGGTCEIVNAFAQELQLDLNKFKADMKGCQEIVQADMRDMQKLGVGATPAFFV
                     NGRYISGAVPIDNFVTVIDEELKKANERIQQGTPKAQYYQQWVVDKGLKTLEAPKQ"
     gene            3187..4539
                     /locus_tag="VMZ53_04420"
     CDS             3187..4539
                     /locus_tag="VMZ53_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS27722.1"
                     /translation="MARPTPIPERNWVNIQWLARLRWAQIAGQATTVLVAQFLLSGQL
                     PIAWLLGVISLGLLSNLAIEMYFFGDRRRAIITPRPVREWHIALLMTVDIGILTGLLY
                     LTGGPENPFAFLYLVQIALATVLVGGRWTWILVGLSFVGFGILLLTHEPLTIPKDSHA
                     YGEWAALGVASAFVVHFLQRITAALAQRERELTEARGLADRQQRLASLATMAAGAAHE
                     LSTPLGTVALAAKELERALTNKHADVELAADARLIREQVGRCRGILDQMAQGAGTVGE
                     SVALATVDELIDETLVGIREQPRVLREIPEEIARASLRLPPRAVSQALRSLVTNAQDA
                     SPPNTAVVITARLDDSQLSVIIRDRGPGISQDVMQRLGEPFFTTKAPGRGMGLGLFLA
                     RAVIEGVGGTLQIDSLEAGGTEVRARLPIDVGARSSVAGAQMVRTSESTPAQVLSKPA
                     "
     gene            4536..5090
                     /locus_tag="VMZ53_04425"
     CDS             4536..5090
                     /locus_tag="VMZ53_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS27723.1"
                     /translation="MSSADQKPSILIVDDDEVYRTRLARAFVDRGYETRTAQDYDSAV
                     VVAQQESPELAVVDLKMPGKSGLELVKALHEIDPATKTIVLTGYGSIATAIDAVRLGA
                     TYYLSKPADADDIIAAFARGEAPPLEPPADHDYKAPSLARAEWEHINRVLSDAGGNIS
                     EAARRLGIHRRSLQRKLQKYPPNK"
     gene            complement(5091..5435)
                     /locus_tag="VMZ53_04430"
     CDS             complement(5091..5435)
                     /locus_tag="VMZ53_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27724.1"
                     /translation="MNKYLAIAIVCAGCADPQPTVRMCNDTGFDINAVAWDTIYSVDS
                     LANGACSDYETPTHTVYAYTRVGFRVQTDEFTIQPIDFVGEDPLGDGAWSYHLTITDY
                     GSRSANVQARRD"
     gene            complement(5432..>5900)
                     /locus_tag="VMZ53_04435"
     CDS             complement(5432..>5900)
                     /locus_tag="VMZ53_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27725.1"
                     /translation="MLREMGEIRVVAALAFVAACDKGKVEPTTGSAQPPPAPLPVAID
                     AMSIDEQILNGYPLTVDVRLCNDTGKTMDAVEWHQSFKATALKTGACTAYQETTGAYG
                     YTYAKFNLGKDEFIIQPIDYMGETPLAPGKWSYHVTIVDYGHRTADIRAKEDK"
BASE COUNT         1040 a         1958 c         1865 g         1037 t
ORIGIN      
        1 agagcctgtc gcgcgaggac ctcctcgaga tcgagcgcaa gatcgccgac gcggagaagc
       61 cggcggccga gaagcccgag aagcccgaga aaccgaagaa ggagcgcaaa tgaccgcgct
      121 cttcaccgcc ggtgcactcg ccaaggcgtg gctcgcgacg ctgctcatga tggcggcgca
      181 aggcacgctg ctcgcgctgc ttgcattcgt gctcgcgcgc gcgggcaagc tgcggcccgc
      241 atggcaggcg gcgatctggc tcgtcgtcac ggtcaagctc gcgctgccgt ggggaccgtc
      301 gatgccgtac tcgctcgccg acgccatcgc gatgttccgc gatcatccgg cgcccgcacc
      361 gatgctgcat atcgcgccat ccgcgccggt cgtcgcgacg acgccagacg cctggcccgc
      421 cgtcggctgg ctgctcctcg ctgcgctctg gctgctcggt gccacgtggg tcgtagtccg
      481 cgcggtactc gcccatcgct cgacgaccgc cgccgcacgc cgcgcgccgc tcgcacctcg
      541 tgatgcactc gcgttgatcg cacagctgtc ggccggcctt cgcgtacgga cgccgaagct
      601 ctcagtcggt gacgcggatg tcggcccgca cgtcgtcggt ctcgttcgta cgattattgt
      661 cgtcccaccc tcgctgctcg gcgacgagcc gctcctccgc gctgcgctgc tgcacgagct
      721 cgcgcatgtg cgtcgtcggg acgcggtcgc tcgcgtcatc cagatcatcg ccgcctctat
      781 catgtggtgg tggccggtcg cgcgcatcgt ccagcgccgt ctcgaagctg cgcgggaagc
      841 cgcgtgcgat gcgtgggcgc tcgagaccgt cgacgttccg cgtgccgcct atgctcgcct
      901 gctcgtccgc atggcgtcgc ttcgtcacgc ggccgcaccg ggcctcgctg cgcatcacac
      961 accgtcgtcg ttgaacaagc gagtcgctgc ggtgctcggt cctccggcgc gtgcgcgtct
     1021 atcgtggctg catcgcatcg cgctcgcggc gtgggtgatc ctcgcgctcg gcggtgctcg
     1081 tagcgcgacg gcgcacacgc acaccgaagc ctgtcactac acgccgcagc tcggcatggc
     1141 tctctacaac tcgtatccgc aagccgacct cgatggagac ggcatgctgt cgcgtaccga
     1201 agcctgcgat ctacaagcag agctgatgcg agatcgtcgt gatgtctcgt cgcgactcac
     1261 gcccgaagcc gaacgcgaaa tggagacgct cctcgccgag cccctctgtt gcaatctgcc
     1321 aaccacagag gtctactcct ctcaggattc cgacacttgc cgaaacgtaa taggagtgga
     1381 gccatgaaag ttctcagtgt gttactcgcg gtgctcgcgc tggctgcgtg ccagaacgac
     1441 accaagaatc tgaacgacaa gatcgacaag atggacaaga agctcgacgc catcctcgcc
     1501 cagcgcggcg gtggtggcgg tgcagctccg cagcgtccat cgcgtcccga gccggatcgc
     1561 tcgaagacgt acgcagtctc cgtcgatggt gatccgttcg aaggcccggc tgatgcgaag
     1621 gtgacgatcg tcgaagcctt cgactatgct tgaccatact gcgaacgcgt ccgtgcgacg
     1681 ctacaagagc tcgagaagaa gtacggcaag gatcttcggg tagtgaagaa gcatttggtc
     1741 gtccatccac agacggccat gagtggagcg ctcgcctact gcgctgctgc gaagcagggc
     1801 aaggcgatgc caatggataa cttgatctgg gacaagggct tcaaggcccg caagttcgac
     1861 gccgatcagt gctggaccgc tggcacctgc gagtcgctca acagctttgc gcaggagctg
     1921 cagctcgacg tcaacaagtt caaagaagac atgaagggct gccagcagct cgttcagaac
     1981 gacatgcgtg acctgcagaa gttcggcgtg ggtgccaccc cgtcgttctt catcaacggt
     2041 cgtttcatct ctggcgcggt gccgatcgat aacttcgtga cggtcatcga cgaggagctg
     2101 aagaaggcaa acgagcgtat ccagcaaggt acgcccgcgg cgcagtacta ccagcagtgg
     2161 gtgatcgaca agggcctcaa ggccctcgag gcaccgaagc agtagccact tacgtcgacg
     2221 aagagagccg ggtcacgccc ggcttttttt gtgctcgttg gttactacac ctgtagtatt
     2281 ctaacgagcg ttgtcgtaag aatgacccag ggagctctca tgaaaaccgt acctatcttg
     2341 ctagccgcgc tggcgctcgc cgcctgccag aacgacacga agaacctcaa cgacaagatc
     2401 gacaagctcg acaagaagct cgacgcggtc ttggcgcaac gcggtggtgg taaccccggt
     2461 gccgcgcagc agcgtccgca gcgccccgag ccggatcgca gcaagacgta ctcggtgtcg
     2521 gtcgccggcg ataacttcga cggccccgcc gacgcgaaga tcaccgtcgt caaggcgtac
     2581 gactacgcgt gcccttactg cgagcgcgtt cgttcgacga tggacgagct ccacaagaag
     2641 tacggcgagg acatccgcgt cgtctacaag cagctcgtcg tgcatccgca gacggcgatg
     2701 accggtgcgc tcgcgttctg cgctgctggc aagcagggca aggcgacgca gatggatgcg
     2761 ctcatctggg acaagggctt caaggcccgc aagttcgacg ccgaccagtg ctggaccggc
     2821 ggcacgtgcg agatcgtgaa tgcgttcgca caagagctgc agctcgactt gaacaagttc
     2881 aaggccgaca tgaagggctg ccaagaaatc gtccaagccg acatgcgcga catgcagaag
     2941 ctcggtgtcg gcgcgacgcc ggcgttcttc gtcaacggtc gctacatctc gggcgccgtg
     3001 ccgatcgata acttcgtcac cgtcatcgac gaagagctga agaaggcgaa cgagcgcatc
     3061 cagcagggca cgccgaaggc gcagtactac cagcagtggg tcgtcgataa gggtctcaag
     3121 accctcgaag cgcccaagca gtaagctgct tcccgcggcg ttgcgcgaag gtgctagggt
     3181 cgcaccgtgg cgcggcccac tccgattccc gagcgcaact gggtcaacat ccagtggctt
     3241 gctcggctgc gctgggcgca gatcgcaggg caggcgacga cggtgctcgt cgcgcagttc
     3301 ttgctctccg ggcagctgcc gatcgcgtgg ctgctcggtg tgatcagcct cggcctgctc
     3361 tcgaatctcg cgatcgagat gtacttcttc ggcgatcgcc ggcgagcgat catcacgccg
     3421 cgtcctgtcc gcgagtggca catcgcgctg ctcatgacgg tcgacatcgg catcctcacg
     3481 ggccttctgt atttgacggg tggccccgag aatccgttcg cgtttctcta tctcgtgcag
     3541 atcgcgctcg cgaccgtgct cgtcggtggc cgctggacgt ggatcctcgt cggactgtcg
     3601 ttcgtcggct ttggcatcct gctcctcacg cacgagccgc tcacgatccc gaaggacagc
     3661 cacgcatacg gcgagtgggc cgcgctcggt gtggcgtcgg cgttcgtcgt gcactttctc
     3721 cagcgcatca ccgccgctct cgcccagcgc gaacgcgagc taacggaagc gcgtggccta
     3781 gcggatcgcc aacagcgact cgcatcgctc gcgacgatgg ccgccggtgc tgcgcacgag
     3841 ctgtcgacgc cgctcggcac cgtcgcactt gcggcgaagg agctcgagcg cgcgctgacg
     3901 aacaagcacg ccgacgtcga gctcgccgcc gacgcgcgcc tcatccgcga acaagtcggg
     3961 cgctgccgcg gcatcctcga tcagatggcg cagggcgccg gcaccgtcgg agagagcgtc
     4021 gcactggcca ccgtcgacga gctgatcgac gagacgctcg tcggcatccg cgagcagccg
     4081 cgcgtgctgc gcgaaatccc cgaggagatc gcacgcgctt cgcttcgcct cccgccgcgc
     4141 gccgtcagcc aagcactgcg ctcgctcgtg accaacgcgc aggacgcctc gcctccgaac
     4201 accgccgtcg tgatcaccgc gcggctcgac gactcgcagc tctccgtcat catccgcgat
     4261 cgcggtccgg gcatctcgca ggacgtcatg cagcggctcg gcgagccctt cttcacgacg
     4321 aaggcacctg gtcgcggcat gggcctcggc ctatttctgg cgcgcgccgt catcgagggc
     4381 gtcggcggaa ctctgcagat cgactcgctc gaggcggggg gcaccgaggt gcgcgcacgc
     4441 ctaccgatcg atgtcggggc gcggtcttct gtcgcaggcg cccaaatggt acgaacgtcc
     4501 gaatcgactc cggcccaagt gctatccaaa cccgcatgag ctccgccgat caaaagccgt
     4561 ccatcttgat cgtcgacgac gacgaggtct accgcacgcg ccttgcccgc gcgttcgtcg
     4621 atcgcggcta cgagacgcgc accgctcagg attacgacag cgccgttgtg gtcgcgcagc
     4681 aagagtcgcc cgagctcgct gtcgtcgatc tcaagatgcc aggcaagtcg ggcctcgagc
     4741 tcgtcaaggc gctgcacgag atcgatcccg cgacgaagac gatcgtgctc accggctacg
     4801 gcagcatcgc gaccgcgatc gacgcagttc gcctcggcgc gacgtactac ctgtccaagc
     4861 ccgccgacgc cgacgacatc atcgcggcat ttgcgcgcgg cgaagcaccg ccgctcgagc
     4921 cgccggccga tcatgattac aaggcgccgt cgctcgcgcg cgcggagtgg gagcacatca
     4981 atcgcgtgct ctccgacgca ggtggcaaca tctccgaggc ggcgcgccgg ctcggcattc
     5041 atcgccgcag cctgcagcgc aagctgcaga agtacccgcc gaacaagtag ctaatcgcga
     5101 cgcgcttgga cgttcgcgct gcggcttccg tagtcggtga tcgtcaggtg atacgaccac
     5161 gcgccgtcgc cgagcgggtc ttcgcccacg aagtcgattg gctggatcgt gaactcgtct
     5221 gtctgtacgc ggaagccgac gcgcgtgtag gcgtagaccg tgtgcgtcgg cgtttcgtag
     5281 tcactacatg cgccgttggc gagcgagtcg acggagtaga tcgtgtccca tgcgacggcg
     5341 ttgatgtcga agcccgtgtc gttacacatg cgcacggtcg gctgtggatc ggcgcagccc
     5401 gcacatacga tcgcaatcgc gaggtacttg ttcacttgtc ctccttcgct cggatgtcag
     5461 ccgtgcgatg tccgtaatcg acgatggtga cgtgatagga ccacttgccc ggagcaagcg
     5521 gcgtctcgcc catgtagtcg atcggctgga tgatgaactc gtccttgccg aggttgaatt
     5581 tcgcgtaggt gtagccgtac gcgccggtcg tttcttgata ggcggtgcac gcaccagtct
     5641 tcagggcggt tgccttgaag ctctgatgcc actcgacggc atccatcgtc ttgcccgtgt
     5701 cgttgcacag gcgtacgtcg accgtcagcg gatagccgtt gaggatctgc tcgtcgatcg
     5761 acatcgcgtc gatggcgaca ggcagcggcg cgggtggcgg ctgcgcagag cccgtcgtcg
     5821 gctcgacctt gcctttgtcg cacgcagcga cgaacgcgag agctgccacg actcgaatct
     5881 cgcccatctc cctcagcata
//
LOCUS       DATLBZ010000083         2708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3796546, whole genome shotgun sequence.
ACCESSION   DATLBZ010000083 DATLBZ010000000
VERSION     DATLBZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3796546"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VMZ53_04440"
     CDS             <1..465
                     /locus_tag="VMZ53_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS27726.1"
                     /translation="FPHLSALENITLAPRVVRGLAPEAAGKRARDLLARVGLGDRGGA
                     YPHELSGGQQQRVAIARALAQDPDVLLFDEPTSALDPEMRDEVMGVIKDLAAGGMTML
                     VVTHEMQFAQDIATRVWVIDKGTIAEDGPPSQVVDAPTSTVAKEFFGRLRRA"
     gene            complement(544..1899)
                     /locus_tag="VMZ53_04445"
     CDS             complement(544..1899)
                     /locus_tag="VMZ53_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27727.1"
                     /translation="MKARGALDAQECAVLREAQKLVIWRAFGCASLVEYMTREMGYTE
                     RAAVERLRVAKVIEEVPALGEALIQGGLSFSGARELSRVVTPETQEEWIAAAQDKNVR
                     QIEDLVSGHKPGDRPTDAPDPALRTKVLRHEVKMAVAELESRARKALQRMRGSSVDDS
                     DFMEACFRAVLDQCAAASSRGAAESRQASREAEPGRDTAMMRCTPAVGDHRELVADEC
                     PDGTRADTHVGAFVVARRVLTEADLERLLAPRYQIAVTTCASCKSGWQHGASSGAANS
                     ASTLLSPPELERVHCDCVDIGDLDADQPDRAKRSIPKALKRHVLHRDGFKCRVPGCNA
                     TANIDCHHVKFVANGGENVISNLVCLCEGHHLAVHAGTLIIEGDATSARFTCSKQSNY
                     KIETRVVECTAALRDRGVPKAAIKAAVAATRTHVGKQDLTAAQWLAIALTKVPTEAST
                     S"
     gene            complement(2507..2656)
                     /locus_tag="VMZ53_04450"
     CDS             complement(2507..2656)
                     /locus_tag="VMZ53_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27728.1"
                     /translation="MPTWVVDRIGARVFYLSLGEERRPAGSGARGPTKGRQRAPRDPA
                     EPGAA"
BASE COUNT          464 a          933 c          838 g          473 t
ORIGIN      
        1 ttcccgcacc tctccgcgct cgagaacatc acgctggcgc cgcgcgtagt ccgaggtctc
       61 gcgccggagg cagcgggcaa gcgcgcgcgt gatttgctcg cgcgagtcgg cctcggcgat
      121 cgcggtggcg catatccgca cgagctctca ggcggccagc aacaacgcgt cgcgatcgct
      181 cgcgcgctcg cgcaggatcc cgacgtgctg ctgttcgacg agccgacgag cgcgctcgat
      241 cccgaaatgc gcgacgaggt gatgggcgtg atcaaggatc tcgcggcggg tggcatgacg
      301 atgctcgtcg tgacgcacga gatgcagttc gcgcaagaca tcgcgacgcg cgtgtgggtg
      361 atcgacaagg ggacgatcgc cgaggatggg ccgccgtcgc aggtggtcga cgcgccgacg
      421 agcacggtcg ccaaagaatt cttcggtcgg ttgcgacgcg catgaacggc aagcgagcgt
      481 cgatcctcac cccgatgtcg ggacgtcgcg cgaccgcttt cggctccggc gaaaccgact
      541 gactcacgac gtactcgcct ccgtcggcac cttcgtgagc gcgatcgcca accactgcgc
      601 agccgtcagg tcttgtttac ccacgtgggt acgagtcgcc gcgaccgccg ccttgatcgc
      661 ggccttcggg acgccgcgat cccgcagcgc cgcggtgcac tcgacgaccc gcgtctcgat
      721 cttgtagttg ctctgcttgc tgcacgtgaa ccgtgcgctg gtcgcatccc cttctatgat
      781 caacgtgccg gcgtggaccg cgaggtgatg gccttcgcag aggcacacga ggttcgagat
      841 cacgttctca ccgccgttcg cgacgaactt cacgtggtgg caatcgatgt tcgcggtcgc
      901 gttgcagccc ggcacgcggc acttgaagcc gtcgcgatgc aacacatgac gcttcagcgc
      961 cttgggaatg ctgcgcttcg cacggtccgg ctgatccgcg tcgaggtcgc cgatgtcgac
     1021 gcagtcgcag tgcacgcgct cgagctccgg tggcgacagc aaggtgctcg cactgttcgc
     1081 ggcgccgctg ctggctccat gctgccagcc gctcttgcag ctcgcgcatg tcgtcacggc
     1141 gatttgatag cggggagcaa gcaaccgttc gaggtcggct tccgtgagca cgcgtcgcgc
     1201 gacgacgaat gcgcccacgt gggtgtcggc gcgcgtcccg tccgggcatt cgtcggcgac
     1261 gagctcacgg tggtcgccga cggctggcgt gcagcgcatc atcgcagtat cccggcctgg
     1321 ctcggcttct cgagaagctt ggcgcgattc ggcagccccg cgcgacgacg cagccgcaca
     1381 ctgatcgagc accgcgcgaa agcaggcttc catgaagtcg ctatcgtcga cgctgctgcc
     1441 gcgcatccgc tgcaacgcct tacgggcgcg cgactcgagc tcggccaccg ccattttcac
     1501 ttcatgccgc aataccttcg tccgcagtgc cggatcgggc gcatccgtcg gccgatcgcc
     1561 cggcttgtgc cccgaaacca ggtcctcgat ctgccgaacg ttcttgtcct gcgcggcggc
     1621 gatccactcc tcctgcgtct cgggcgtgac aacgcgactc agctcgcgcg cacccgagaa
     1681 ggataagccg ccctgaatca gggcctcacc cagcgccggt acctcctcga tgaccttcgc
     1741 cacgcgcagt cgctccacgg ccgcgcgctc ggtgtagccc atctccctcg tcatgtactc
     1801 gacgaggctc gcgcatccga atgcgcgcca aatcacgagc ttctgcgcct cacgcaagac
     1861 cgcgcactcc tgcgcatcga gcgcgccgcg cgctttcaca atgcgtttca gtgttcggtg
     1921 gactcgatcg cacttcgcat cgtgctcgct tctactcgca ctcgcactcg cactcgcact
     1981 cgcactcgca ccaacccaag cagccacatt ttcgctcgat ccttcacgca acatcggtcg
     2041 cctctcctgc ttcgcttcgt gtgaagcact tctaccattg cgaaatccga cgctatgcgc
     2101 ggcgctacgc gctgcgaatt cgcatttgtt cgtcgagaaa cggactcatc gaaaggattg
     2161 cgctgcatgc agccccaacg ctgctcgtgc gcagttggat cgcatttgca aagagggggc
     2221 gcgtgggcga agaaatgccg tcaacggaaa tcgtcagcga gaggagatct cgccgcgcga
     2281 aacatggacc gaacattgcg agcgtccggt tctcggacat ctattgttcg cgctcgtggt
     2341 gaagccgctc acgaaaacta gaaagcgccg aaggaaagca gatgaggaac acgacccacg
     2401 tgggcatcgg ctcgcatgcc cacgacgacg accgcgtcga gctcacgagg cagcgatcgc
     2461 acatcgcgac ccacgtgggc atcgattcga tatccgaaca cgctgactaa gccgcgcctg
     2521 gctcggcggg atcgcgcggc gcgcgctggc ggcccttggt ggggccgcgc gcgccgcttc
     2581 ccgccggccg gcgctcttcg ccaagtgaca aatagaacac gcgagcaccg atgcgatcta
     2641 cgacccacgt gggcatcgca tcgcacgccc gatgacctcg agctgacaat caggcaccga
     2701 ttgcggag
//
LOCUS       DATLBZ010000084        20658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3016806, whole genome shotgun sequence.
ACCESSION   DATLBZ010000084 DATLBZ010000000
VERSION     DATLBZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20658
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3016806"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VMZ53_04455"
     CDS             complement(<1..1179)
                     /locus_tag="VMZ53_04455"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HUS27729.1"
                     /translation="MKVHLIGIGGTGMGAVAGLLAAAGHEVRGSDAAVYPPMSEQLAA
                     LGVPVMMPYGAQNLDWTPDLVVVGNVHGKDHVEVKAAQERGLQLTSFPAVIGERLLDG
                     KHSIVVAGTHGKTTTTSLLAHILLEAGRDPSLFVGGVPVALGQGWRLGKGGPFVIEGD
                     EYDTAFFDKGPKFAHYRPKTAILTSVELDHVDIFPSFDAVRDTFKKLISLMPSEADGG
                     LLVVCAESRDALAVAKNAHCKVEQYCVLAGDNDNDKSGSFEVDTDAGLPKDVTWFAKG
                     LEVGKSGRIAFEVFHKGERYERFETLLVGRHNVGNCVAAIAVAHSLGVPIKDIQRGIA
                     SFAGVRRRQELRGIAGGVTVLDDYAHHPTAVRETLKALRKRFPKRRLIAVYEPRSATS
                     R"
     gene            1262..1612
                     /locus_tag="VMZ53_04460"
     CDS             1262..1612
                     /locus_tag="VMZ53_04460"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS27730.1"
                     /translation="MDRERYERRQYFRGKARPGRVLPLRFKTTLHPAWITAETRNIGV
                     GGAYIATGNVQEVGTPLTVELSLPTSDRVFTLPSIVRWSSAADPSGMGIQFVGVDVDI
                     LLELNDYFATLTSV"
     gene            complement(2398..3363)
                     /locus_tag="VMZ53_04465"
     CDS             complement(2398..3363)
                     /locus_tag="VMZ53_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27731.1"
                     /translation="MRAALFVAAFVAVVPSARADDTFESKAAGAQRVRRVEDLVWALT
                     ATCDQNDDTQNRQCRKLRDAKAAELANATLLVDADKDAFSVGAWNAQKKSVPLALDAC
                     IRCKGIDVAGKTWFVVGTKDGIAAPAWKSAKLVTGSLHDNARPFGDDASAKTWAKTAT
                     ASNVQLLVKVGAKPKQSIENKNVLALEVLGYRVFSPCDGSVVAASPKSSAVELEKADK
                     QACAPAPATPTGEAIESLTPAMISQTMKPVAEQAAQCGQKHGVHGAGKVKLTVSADGS
                     VAAVELTGAFASSKAGECIEALAKSAAFPKTKKAKTVFSYPIKLP"
     gene            complement(3363..3899)
                     /locus_tag="VMZ53_04470"
     CDS             complement(3363..3899)
                     /locus_tag="VMZ53_04470"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HUS27732.1"
                     /translation="MAHIDVFKSWAETIRQDIDAYKALLESGKADEKARRLAGGALSY
                     LVSKMDLIPDWNEGIGVIDDVMVLRVCAALSQDYDRGGLPGAAEASLERMANQSEKIS
                     EFLGGPIYDKLKSYCSKLNELKIRGATPATLIEDEAARKALYTALDEEIEKTVPIVIN
                     DPKDAELRLKAYLTHKLQ"
     gene            complement(3966..4268)
                     /locus_tag="VMZ53_04475"
     CDS             complement(3966..4268)
                     /locus_tag="VMZ53_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27733.1"
                     /translation="MSKFALAFVLAAVSACGGDDSQATPDAPSGPMCTKALYDNCATN
                     ADCTSGNCHFFMQSNFSICTQACSTTTPCPVDSTGVAGQCNNMGICKPAAANNCHL"
     gene            4337..4627
                     /locus_tag="VMZ53_04480"
     CDS             4337..4627
                     /locus_tag="VMZ53_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27734.1"
                     /translation="MRSLLILTLLAACSGDQPADVDANPNGPMCTKAAYDLCTTEHDC
                     TSGLCHYFNNEFMVCTQSCDTANPCPNDRNGTPGTCNGMSICKPAVANMCHL"
     gene            complement(4634..6385)
                     /locus_tag="VMZ53_04485"
     CDS             complement(4634..6385)
                     /locus_tag="VMZ53_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27735.1"
                     /translation="MAKTESTAVNELISLVQGGAPAPADPSADLFSEPKAAKAVQPPR
                     MTTTIPAMRGAGEVAPLPRGRAPQSTSTHSIADQPPPAVRMTTASPARGTTIPPIGRA
                     STPPPTPDAKVTNSSPTLPSPRITRPSLPPPRTSYPTVPAPKQSLSGMPELRNTPRAT
                     LPPDAMPALQGALPDVISSDNLFEIDVETPKPRKSLHDETWVGSRQNSRKRELARMLV
                     VPAIIAASVGVAIGVYISYRGGHTKPAPHASALPSATTPAIESPAKEIQLPVTSTAPD
                     PNAESPNAATASAGAGEPQPPTKEEDAAKQQAAAGLAAANANDPAAEPSLTRDAPVNG
                     AAQSAVATTTPTAAQAIDSPPPAVQEIRTTKGIIKLVDVRINSKPAGATVMLVDDGKT
                     SFLGTTPLATSVDASKSYDVIFTLEGRPTQMAHLDPATSARIDMQLPRASHHEAKPAV
                     VAAPLADVVEKKAVVEKKAVEKKAEPKKAAKTTASFDEAAELASLGEAVPAKKAVAKT
                     DAGGEGTLMLSSKPPCEIYIDGKATGLTTPQRSIALSSGSHKVTLVNTAENIKKTVSV
                     QITADKPTKLIQNLMTE"
     gene            6699..8096
                     /locus_tag="VMZ53_04490"
     CDS             6699..8096
                     /locus_tag="VMZ53_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27736.1"
                     /translation="MERVVRRLGSRCIPLLGRELLSPQAARRDAARDVLALLAKESDS
                     RERVLAELRRIANDAKREDGASDEAKVAALGLLAEHGERTAARFADPGAIQRRSALAL
                     ASSLDNEADVAAAADMMIHQLGPLEIAHLIEVMVDASRGAAYHLAVELCARLDLDAAT
                     RECISELALGGSAPLPVVRGAQKPAQVAVLVDPAHKVVVVVSRKISARRWRRWAVLIG
                     AHGAIDDCVHDERTASSGNVDADHAPLIARLVADGYQVASTELERARGIVAAAARQTT
                     STSDEAAAKLPSAYYLGRDLLDLGEAHLGGRVHAHPTSTSLGRAVELIADGDHTRAQV
                     LLARCADSADVAAATGASLLAQQRIADALPHLLRAAELEPEFPLHHWNLASVLHHLGD
                     PGSMAACYAALRRFLRASEQPTGLFADPDQPARVGLAIRLIADLERTARLTGLKLDTG
                     TRRKRRAPKREAAKR"
     gene            complement(8097..9731)
                     /locus_tag="VMZ53_04495"
     CDS             complement(8097..9731)
                     /locus_tag="VMZ53_04495"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS27737.1"
                     /translation="MRNSLAFVLTAGLVACGGSTKGPENPSGGGGGGGGGGGAKPSGP
                     GDVSFEVPTIQVNGVLFEPEALGSPGMPLAYPKKKTTTDKQRQTYDKAAKGKDVVQRQ
                     AQAAVLATLIYDDAKKAKDEAEKQKMWSEARGVLRDSVTAAGEGKGDPTDLRMLGSFE
                     LLLEDYAGAEKAWSALVASDPKDKELPTFKAWWAYALLRQYKSADALNVIANEQITDK
                     TPELAYVMAWAKFRTGDDKGAWQAISLAAKGGTSLFPHDPLLRDMLLFAGRTGASIDE
                     AVASLQPVFGKAQQYELLAKLGLESYQYAGRWQDGVMVLEKAVTASGGKVPVNDMPVL
                     RFEQADYTVRLDDPVASAKFGKLAVDALPACGAKCPPQDMATIVYSVYLMGQLFHVLY
                     ATAHDDRYYQPAHDLYQATVSKLTDDKQRGDAQKNADALERSFKAMKAGVGTHSKDAI
                     AALLNRHNQEIQACFERGLAANPKLSGTVTLNLESDQTGAIKGASTEPKAGTEGMSMV
                     AGCVETHAKAWKLPKRAQAGSTRIKMSYSMSVAAKK"
     gene            9788..10003
                     /locus_tag="VMZ53_04500"
     CDS             9788..10003
                     /locus_tag="VMZ53_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HUS27738.1"
                     /translation="MRRLSFVVVVLTALASNGCAVVKAHQRGNLAKRSMTNDRDPGEA
                     RFGQHERGSREGADGGTGQPGGGCGCN"
     gene            9988..11127
                     /locus_tag="VMZ53_04505"
     CDS             9988..11127
                     /locus_tag="VMZ53_04505"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HUS27739.1"
                     /translation="MRLQLIAVVLVVAAGTAYADGTLSMRGVYYKERSTRVVQPMLDA
                     MFEVGAHGLASGHLAVDAITSASPSSGAEAEAFTEQRYEGGAGYAHEVEGPDDSWIDV
                     VRVGGEAKVSKEPDYRSIYAGARLEAELAQKNATLGLGGGVSLDEINNTGAQSPMGGP
                     KLLCDNKLTMATETTCPLNTYSVFTSASQLVDKNALIGVSYDLAKAHGFTSNAYRQVI
                     TTSGYVPEKHPNDRLRQAVAISGRYYWPRAKTAFIAAYRFYWDDWKIHAHTPELRIIQ
                     EVGTTVDASFRYRYYKQNAAFFYEKPYPDQSVQMSQYLTDDPKMTAFDGHLMEAKLGI
                     LGETFGLNGRWAGARIEGILEYIVQNNRFGNAVVAHASLTVPFDY"
     gene            11096..11644
                     /locus_tag="VMZ53_04510"
     CDS             11096..11644
                     /locus_tag="VMZ53_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27740.1"
                     /translation="MHRLLFLSTISVAALVGCEGCGDDSCGPGDAPATGLIVGDQDQK
                     LTFGNLSSSANNDCPDPSAPAGVVSLTVSGSQTDAPTGGSGLLTLCIPRPDQLAGTVQ
                     LGTGVKIIDLHGQANGCMYSIEATRPITGTAYGSGVCNNGQDGAGYALTFDGHISLKR
                     ECPTATDTIAVNVSGEVAVAGH"
     gene            complement(11641..13353)
                     /locus_tag="VMZ53_04515"
     CDS             complement(11641..13353)
                     /locus_tag="VMZ53_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HUS27741.1"
                     /translation="MKRLVFVMALAFPTAAHAETRCASIEMTPSEKLQIVAWIETASG
                     EYVDTTYITQTVGRFGLGNRPGRWDFNSGPMWPYGRRLATFPVWAHRHGHTFPSVVFQ
                     NCCGHMAGSTGGSVPVTDDPNYCFSVINDSTKSSPNDREFADCGENNLSHAFDESSRE
                     LHYCQPFMASDPKWAKADAMTCATVAHTDKGKFSTTSTSLYPPRLDLIYNPQTDAPSV
                     EQFKTMAPFDAVSQATPPGGVAQAIAWPVPDALPAGSYVMFVEVAKEFDHNATFSEAA
                     HPAPPAVGPMGIQWSSFGLPYLGQPSVVYKVPFEITTGQTTASTSDFIGYSAIDGSDG
                     NIRPPDGSITTDTPGSGASRLQLVSDGSAMYRVRVSVSPNDSSHVPSAPANIKAAELG
                     TQTITLAFTAPGDGTSTVAGYDVRIKANGVITPETFDDAMKVGVAATLVRPGQTQTLE
                     LMGLLPETDYSVAIRAFDACRNNGDIAIVNVRTADRIAGEVDACFIATAAYGSVMAND
                     VELLRHFRDSLLESTVLGELAVEAYYTFGPPVAGMIGESDLLRTMVRAMLAPIVDAVR
                     KLSV"
     gene            complement(13350..15122)
                     /locus_tag="VMZ53_04520"
     CDS             complement(13350..15122)
                     /locus_tag="VMZ53_04520"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HUS27742.1"
                     /translation="MPAARWTAIGAFTALVGTAHAEVHECHVIDVDFTPAENTSSESS
                     RHEPSQMVVWLETADGHYVSTLYITAQTGRFGLGNRPGRYDFKSGPMWPYGRRITTFP
                     VWAHRKPERYPEVVFQNMEGFDDCCYDDCINGAHQTPEFCTDSCTFEDTCDGLMGMDF
                     MFCGDNNLSHYSCDSSLETHYCRPFNLADPNDAVKWQAADAMSCSSPAFTDKGKLSTL
                     RTSLYPPRLDVVKTNVDSPSVDMYKQLTGFDAVSRATPMPGTRETVTWPLPANTPAGD
                     YVVWAEVSQAFDHNTTYNPTTQPPPANTGSQAITWAEYGLPYRGQPSIVYKVPFTLGT
                     EQLTVTTDAYVGYGAPDGTDGNVRAPDSTITTDTPGSGASRLQLISEGGALYRLRVVA
                     RPEFDYAAPGAPEAGEVVAVTPTTASLRFAAPGDDGQVGKVSAYEVRYLVNQELTAEN
                     FETATRATTSIAIGNPGELQSIELVGLLPETEYSIGVRALDDCFNSGPLTVISVRTAD
                     RPVGTVDACFIATAAYGSMMANDVELLRDFRDATLRSSVLGELAIETYYTFGPPVAGV
                     VGQSDVLRQLARGMLQPIVEWVKR"
     gene            complement(15133..16686)
                     /locus_tag="VMZ53_04525"
     CDS             complement(15133..16686)
                     /locus_tag="VMZ53_04525"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HUS27743.1"
                     /translation="MTVAVDFTPTDNLQIVAWLEKTDGTYVDTAYITNKTGRFGLGNR
                     PGIPNFNTGSASNDTWPYGRRVQTFPVWAHRHGHQFPVVIFQNLDHRNLSHPFAQSSP
                     ELPPPYGRPIQPNENGFDAGTTASASFTDKGRFAIGSSETDDGSTFSLYPPRSDIERR
                     PSSDSPAVDMFRANNPFDAVSQATPFGGLSATAVWSAPESVDYGDYVLYVEASKTYDF
                     NATYNETTYPPLTDIPWKEYGKAWRGQPSVVYSVPISIASTPTRATTTDYVGYGDPDG
                     KNGTLNPPDATITTTTPGSGASRFQLVSDGAEMYRVRVRATPEFDSTPPAEVGSIEAV
                     GITSTSGTLQFLAPGDDGSIGAVTGYEVRVRVGSPVTDENFKESMPVTATLVPTSPGE
                     MQMVTISGLLPETEYFVGVKAYDNCFNRGPLATTTFKTNYREAPEVPWCFIATAAYGS
                     VMANDVAMLRRFRDGVLGRSVLGELAIETYYTFGPAVAGVIGESDLLRASVRGFLVPI
                     VDTVRKLSY"
     gene            16808..17653
                     /locus_tag="VMZ53_04530"
     CDS             16808..17653
                     /locus_tag="VMZ53_04530"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HUS27744.1"
                     /translation="MARTPSAEYLILDIETIPDVERWKRPDLPSVTADTQAPADAAAP
                     DVVRPAEPPRAGRGDRGGFPPSWAHRIIVIGCLWLDHSYRLKRLGVVGDPTGGEGSPD
                     HRERVLLEDFSRFVGRARPVLVTYNGRSFDLPVIALRALCHGVSIGWYYRERDVRYRY
                     SEQGHLDLCDWLADHGATRSGSLDAVARLIGLPGKVGVDGSQVEGLYQSGQLASIQRY
                     CLADVAQTALLFLRFRLLQGVLRSDQYQDATNALLDALAADGRVNDVLEGTDRAHLLG
                     PTPTA"
     gene            17653..18498
                     /locus_tag="VMZ53_04535"
     CDS             17653..18498
                     /locus_tag="VMZ53_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HUS27745.1"
                     /translation="MPAKSAKSEIAELAKRRLPPAWFQRDVVIVAKELVGCALVHRNR
                     AGLIVETEAYMGPDDLASHARFGPTARTSVMFGPGGVSYVYLCYGIHQMFNIVTGTEG
                     QGQAVLIRAVAPLVGLPDDPSVGRGPGKVTTALKLDRRHDRKDLASHSRFGPTARTSV
                     MFGPGGVSYVYLCYGIHQMFNIVTGAAGDGQAVLIRAVAPLVGLPDDPSIGRGPGKVT
                     KALALDRSHDRKDLADGTLYVARYLAPPRVAKGPRVGVAYAGPWAERPYRFWWRDHPA
                     VSRVR"
     gene            complement(18509..19345)
                     /locus_tag="VMZ53_04540"
     CDS             complement(18509..19345)
                     /locus_tag="VMZ53_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27746.1"
                     /translation="MTKAVTRTWYRIALARAGAPTQLVAASGEHMGQAIAEARDHVAG
                     SWPVAVEHAQGQDIPLGESVGKGHVLMLGDAPADTPTFEWPTGVLPSLAAPGSAFAGA
                     APGWVMRPESKLLVIEAQTTDDKLTDLFLGMVERLPAGDNLEVRVLDHFEDAGTTDVW
                     LTSRVTAKKIIRFIDDHDTELIGNGHLELSIYVRAQNATLRLSEHKTVLWLAEGRALE
                     VEACGWLKDLGVPPAESLVTVREGAHFHYRPAKSRDRKKLGEELYRQRMRKVDSVATH
                     RA"
     gene            19407..19733
                     /locus_tag="VMZ53_04545"
     CDS             19407..19733
                     /locus_tag="VMZ53_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27747.1"
                     /translation="MNKLSMFACGIVLAIGCGSKDPVDKAIAGLEDWKAKMCACKDKA
                     CADKVHEDYKKWENDVMEKSLKDVDKKSVSKDKMEKFDKLDDARKDCRRKFNEPEAPP
                     ATPPTP"
     gene            complement(19734..>20658)
                     /locus_tag="VMZ53_04550"
     CDS             complement(19734..>20658)
                     /locus_tag="VMZ53_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27748.1"
                     /translation="AVFRAMDIQPDPVTGEPVCPRVQFAANGLPMPVLPICDDPDPFA
                     CARVPAVGGRTYYHPGDDETVVDLGCTNLEQLTDAAVCIGENRVTVNATVNDFDDAAS
                     VDKTVADRLNVDIGEPKPFADTYVLGPEESDPLERSTASTPSWGALIERKFMNIACIA
                     VREDGAQTTTALTCNAKAVTENDYDSLLGYYLRKATLAQILSAIGMTEFPAQGGLVVG
                     MVVDENFNPRAGEPVSCPAGCTLQYLNNGRTGVITGGVTSASGIFVSTDAPFGTQFSL
                     PTSPQRADTIGGVVEGKVTIVVLQYRELEPL"
BASE COUNT         3356 a         6862 c         6794 g         3646 t
ORIGIN      
        1 gcggctcgtc gcggagcgcg gctcgtagac ggcgatcagg cggcgcttcg ggaatcgctt
       61 gcgcagagcc ttgagcgtct cgcgcacggc cgtcgggtga tgcgcgtaat cgtcgagcac
      121 ggtgacgcca ccggcaatgc cacgcaactc ctgccggcgc cgcacacccg cgaagctcgc
      181 gatgccgcgc tggatgtctt tgatcggcac gccgagcgag tgcgcgaccg cgatggcagc
      241 gacgcagttg ccgacgttgt ggcgaccgac gagcagcgtc tcgaatcgtt cgtagcgctc
      301 gcccttgtgg aacacctcga acgcgatgcg gccgctcttg ccgacctcga ggcccttcgc
      361 gaaccacgtg acgtctttgg gcaggccggc gtcggtatcg acctcgaagc tgccggactt
      421 gtcgttgtca ttgtcgccgg cgagcacgca atattgctcg accttgcagt gcgcgttctt
      481 cgcgacggcc agcgcgtcac gtgactcggc gcagacgacg agcaagccgc catctgcctc
      541 cgacggcatc agcgagatga gcttcttgaa cgtgtcgcga accgcgtcga acgacggaaa
      601 gatgtcgacg tgatccagct cgacgctcgt caggattgcg gtcttgggcc ggtagtgcgc
      661 gaacttgggg cccttgtcga agaatgcggt gtcgtactcg tcgccttcga tgacgaacgg
      721 gccgcccttg ccgagccgcc agccctgccc gagcgcgacc gggacgccgc cgacgaacag
      781 cgacggatcg cggccggctt cgagcaggat gtgcgcgagc agcgacgtgg tggtggtctt
      841 gccgtgcgta ccggcgacga cgatcgagtg cttaccgtcg agcagccgct cgccgatcac
      901 cgcggggaac gacgtcagct gaaggccgcg ctcctgcgcc gctttgactt cgacgtggtc
      961 cttgccgtga acgttgccga cgaccacgag gtccggcgtc caatcgaggt tctgcgcgcc
     1021 gtacggcatc atgaccggca cgcccagcgc ggcgagttgc tcgctcatcg gcgggtagac
     1081 ggcggcgtcg gatccgcgaa cttcgtggcc cgcggccgcg agcaagcccg cgacggcgcc
     1141 catgcccgtc cccccgatgc cgatcagatg caccttcacg atcgggcgcg accctacaag
     1201 cgtcccggcg gcgggtaaag cgttttgccg cccggaaaaa cagatatgat ctcgtaggga
     1261 aatggaccgg gaacggtacg agcggcggca gtactttcgg ggcaaagcac ggcccggccg
     1321 cgtgctgccg ctgcgcttca agacgacgct tcacccggcg tggattacgg ccgaaacgcg
     1381 caacatcgga gtcggcggcg cgtacatcgc gaccggcaac gtccaagagg tcggcacgcc
     1441 gctgacggtc gagctgtcgc tgccgacgtc cgatcgcgtg ttcacgctgc cgtcgatcgt
     1501 gcgctggtcg agcgccgcgg atccatcggg gatgggcatc cagttcgtcg gcgtcgacgt
     1561 cgacatcctg ctcgagctca acgactactt cgcgacgctg actagcgttt gacgtcggtc
     1621 gactcgaaga tcttgtcggc gctcttgggc acgaatccct ggaacagctc gccggtcggc
     1681 tgcggataac gccgcgcgaa ctcgtagtag caggatggga tgccaccgtc gcgtccgcaa
     1741 acgcgacctc catctcgtcg gcgcgcgtcg acgactgctc gagccggtct gccggcgtgc
     1801 ctttgatcgc gccaccgctc tcgttgagcg tgaagccgtg atcgacgagg aacgcattga
     1861 gcgagcggag atccggaaac gtggtgagtg cgttcacgtt gaccgtgaag tgattcacgc
     1921 ggaagccgaa cgctgcgacc cacgccgcgt actcgctctc acgcaagagc gagtcgtact
     1981 cggctttgcg caccggccac ggccgtcctg catacggaag atccatgcgc tgcccgaagt
     2041 cgctcggcgc ctcggacacg aggttctcga tgatctgcga cgacgatgcc gacagctcgt
     2101 agatgcacag ctcgctgatg aagaccttcg gcagcgtctc gtcggcatgc ttccagtagc
     2161 gtgcgatgag cttcttgtcc tcgaatcggt agtagtccgg ctgcacgacc cagccgagcg
     2221 cttcgaacgg ccgcgcgagc gcttggatgc cgatgcccgg catgccgaac gtacgcagcg
     2281 cgatgtgatc gttctgcacg gtctcgccgc gctccgcgag cagcttgtga atgcgctcgg
     2341 cctgcggtgt ggtcgcgacg taatcggccc acagcgccgt taagagatca gcctcggtca
     2401 cggcaacttg atcggatagc tgaagaccgt cttggctttc ttggtcttcg ggaacgccgc
     2461 cgacttcgcg agcgcctcga tgcactcacc cgccttactg ctcgcaaacg cgccggtcag
     2521 ctcgacggca gcgacgctgc catcggccga cacggtcagc ttcactttgc ccgcgccatg
     2581 cacgccgtgc ttctggccgc actgcgcggc ttgctcggcg accggcttca tcgtctgcga
     2641 gatcatcgcc ggcgtgagag attcgatcgc ttcgccggtc ggcgtcgcag gcgcgggagc
     2701 gcacgcttgc ttgtccgcct tctcgagctc gacggcactc gacttcgggc ttgcggccac
     2761 gacgctgccg tcgcacggcg agaacacgcg atagccgagc acctcgagcg cgagcacgtt
     2821 cttgttctcg atcgactgct tcggcttcgc gccgactttc acgagcagct gcacgttcga
     2881 cgcggtcgcg gtctttgccc acgtcttcgc gctcgcgtcg tcgccgaacg gccgcgcgtt
     2941 gtcgtgcaac gagccggtca cgagcttcgc gctcttccat gccggcgccg cgatcccatc
     3001 cttcgtaccg acgacgaacc atgtcttgcc ggcgacatcg atgcccttgc agcggatgca
     3061 cgcatcgagc gcgagcggca cggacttctt ctgcgcgttc cacgcaccga cgctgaacgc
     3121 gtctttgtcg gcgtcgacga gcagcgtcgc gttcgcgagc tccgcggcct tggcatcgcg
     3181 cagcttgcgg cactggcggt tctgcgtgtc gtcgttctga tcgcacgtcg cggtcagcgc
     3241 ccacacgaga tcctcgacgc gtcgcacgcg ctgcgcacca gcggccttcg actcgaatgt
     3301 gtcgtcggca cgcgcgctcg gaacgaccgc cacaaaagcg gcaacgaaga gtgccgcgcg
     3361 cattactgga gcttgtgcgt gagatacgcc ttgaggcgca gctccgcatc tttggggtcg
     3421 ttgatgacga tcggcacggt cttctcgatc tcttcgtcga gcgccgtgta cagcgccttg
     3481 cgcgccgctt catcctcgat gagcgtcgca ggcgtcgcgc cgcgaatctt cagctcgttg
     3541 agcttggagc agtacgactt gagcttgtcg tagatcggac cacccaggaa ctcgctgatc
     3601 ttctccgact gattcgccat gcgctcgagg ctcgcctcgg cggcgccggg caggccaccg
     3661 cggtcgtagt cctgcgagag cgctgcgcac acgcgaagca ccatcacgtc gtcgatgacg
     3721 ccaatcccct cgttccaatc ggggatcaga tccatcttgc tgacgagata gctcagcgca
     3781 ccgcccgcga gcctgcgcgc tttctcgtcg gccttgcccg actcgagcag cgccttgtac
     3841 gcgtcgatgt cctggcgaat cgtctccgcc cagctcttga agacgtcaat gtgtgccacc
     3901 agcggcctcc ttgcgggcaa cgtacccccg acgacggagg cgttcaatgg ccccaggcca
     3961 cgtcgtcaga ggtggcagtt gttggcggcg gcgggtttgc agatgcccat gttgttgcac
     4021 tgccctgcca cgccggtcga atccaccgga cacggcgtcg tcgtgctgca ggcctgcgtg
     4081 cagatgctga agttcgactg catgaagaag tggcagttgc cgctcgtgca atccgcgttc
     4141 gtggcgcagt tgtcgtagag cgccttcgtg cacatcggtc cagatggcgc atccggcgta
     4201 gcttggctgt cgtcgccgcc gcaggcagat acagcagcga gcacaaaggc gagcgcgaat
     4261 ttcgacatgc ggcgatcgta cgcggagcgc gcacaaaatc gccaatgaca cgtcgatgac
     4321 aggctacgat gatcacatgc gtagcttgct gatcctgaca ctcctcgccg cttgcagtgg
     4381 tgaccagcca gccgatgtag acgcgaaccc gaacggcccg atgtgcacga aggccgccta
     4441 cgacctctgc acgaccgagc acgactgcac gagcggcctc tgccactact tcaacaacga
     4501 gttcatggtc tgcacgcagt cgtgcgacac ggcgaacccg tgcccgaatg accgcaatgg
     4561 cacgcctggc acctgcaacg gcatgagcat ctgcaagccc gccgtcgcga acatgtgcca
     4621 cctctgagga gcgctactcg gtcatcaagt tctggatcag cttcgtcggc ttgtccgcgg
     4681 tgatctggac cgagacggtc ttcttgatgt tctcggccgt gttgacgagc gtcaccttgt
     4741 gcgagccgga cgacagcgcg atcgagcgct gtggcgtggt gaggcccgtc gccttgccgt
     4801 cgatgtagat ctcgcacggc ggcttgctcg acagcatcag cgtgccttcg ccgcctgcat
     4861 cggtcttcgc gactgccttc ttcgcgggca ccgcttcgcc gaggctcgcg agctccgcgg
     4921 cttcgtcgaa cgacgccgtg gtcttcgcgg ctttcttcgg ctccgccttc ttctcgacgg
     4981 ctttcttctc tacgacggct ttcttctcga cgacgtcggc gagcggcgcg gctacgacgg
     5041 ccggcttcgc ttcgtggtgc gaggcgcgcg gcagctgcat gtcgatgcgc gcggaggtcg
     5101 cgggatcgag gtgcgccatc tgcgtcgggc ggccttcgag cgtgaagatc acgtcgtagc
     5161 tcttgctcgc gtcgacactc gtcgcgagcg gcgtggtgcc gaggaacgac gtcttgccat
     5221 cgtcgacgag catgacggtc gcgcccgcgg gcttgctgtt gatgcggacg tcgacgagct
     5281 tgatgatgcc cttcgtcgtc ctgatctctt ggacggcggg cggcggcgaa tcgatcgctt
     5341 gcgccgcggt cggcgttgtc gtcgcgacgg cagactgcgc ggcgccgttg accggcgcgt
     5401 cgcgcgtgag cgacggctcg gcggctggat cgtttgcgtt cgcggcggcg agccccgcag
     5461 ctgcttgctg cttcgcggcg tcttcttctt tcgtcggcgg ctgcggctcg ccagcgccgg
     5521 cggaagcggt tgccgcattc ggcgattcgg cgttcggatc cggcgccgtg ctcgtcaccg
     5581 gcagctggat ttccttcgcc ggcgattcga tcgcgggcgt cgtggccgac ggcagcgcgg
     5641 acgcgtgcgg ggcgggcttc gtatggccac cgcgatacga gatgtagaca ccgatcgcga
     5701 cgccgaccga tgcggcgatg atcgcgggca cgacgagcat gcgcgcgagc tcgcgcttgc
     5761 gagagttttg gcgcgagccg acccacgtct cgtcgtggag cgacttgcgc ggcttcggcg
     5821 tctcgacgtc gatctcgaac aggttgtcgc tcgagatcac gtcggggagc gcaccctgga
     5881 gggcgggcat cgcgtcgggt ggcagtgtgg cgcgcggcgt gttgcgcagc tccggcatgc
     5941 ccgacagcga ctgcttcggc gccggcaccg tcgggtacga ggtgcggggc ggcggcagcg
     6001 acggccgcgt gatgcgcggc gacggcagcg tcggcgacga attggtgact ttcgcgtccg
     6061 gcgtcggcgg cggcgtcgac gcgcgaccga tcggcgggat ggtcgtgccg cgcgcgggcg
     6121 atgcggtcgt catgcggacg gccggcggcg gctgatcggc gatcgagtgc gtcgacgtgc
     6181 tctgcggcgc gcgaccacgc ggcagcggcg cgacctcgcc cgctccacgc atcgcgggga
     6241 tcgtcgtcgt catccgcggt ggctggacgg cctttgccgc tttgggctcc gagaagagat
     6301 cggcgctcgg gtccgcaggc gcaggtgcgc caccttggac cagactgatc agctcgttca
     6361 ccgcagtcga ctcggtcttc gccatgtgag ctcccgggaa actgaaaacg ggttcgctat
     6421 cgcgggcgac cggagaattg cgaatccgct tcggagtcac aagcgaaact ggatgacaga
     6481 gatcgatctg agtgcgaatc gggagtgatg tgcttacgtt tcgatcactt cgacgatttc
     6541 gacctaagat cgcgaaattt cattgacagt tatatgacca tatacgtagt gtatggccag
     6601 gaatggggct agagagcgca ttcgtcgacc ttcgcaggct cgccgattgg ttcgcatctc
     6661 tccacggcca gaaccctgtc gaccgacccc gagcgctcgt ggaacgagtg gtccggcggc
     6721 tgggcagtcg atgcatcccg ctgctcggtc gtgagctcct ctcgccgcag gccgcgcgtc
     6781 gtgatgccgc tcgcgatgtg ctcgcgctgc tcgcgaaaga aagcgattcg cgcgagcgcg
     6841 tgctcgccga gctgcgccgg atcgcgaacg atgcgaagcg cgaagacggc gcgagcgacg
     6901 aagcgaaggt cgcagcactc ggcctgctcg ccgaacatgg cgagcgcacc gccgcacgct
     6961 tcgcagaccc gggcgcgatc caacgccggt ctgcactcgc gctcgcttcg tcgctcgaca
     7021 acgaagccga tgtcgccgcc gcggccgaca tgatgatcca ccagctcggg ccgctcgaga
     7081 tcgcgcacct catcgaggtg atggtcgatg cgtcgcgcgg cgctgcgtat cacctcgccg
     7141 tcgagctctg tgcgcgcctc gatctcgatg ccgccacgcg cgagtgcatc agcgagctcg
     7201 cactcggcgg ctctgcccca ctgcccgtcg ttcgcggcgc gcagaagccg gcgcaagtcg
     7261 cggtgctcgt cgatccagct cacaaggtcg tcgtcgtcgt gagccgcaag atttccgcgc
     7321 ggcgttggcg tcggtgggcc gttctcatcg gcgcgcacgg cgcgatcgac gactgcgtgc
     7381 acgacgaacg caccgcctcc tcgggcaacg tcgatgcgga tcacgcgccg ctgatcgcgc
     7441 gcctcgtcgc cgacggctat caagtcgcca gcaccgagct cgagcgtgca cgcggcatcg
     7501 tcgcggcagc cgcgaggcag acgacgtcga cctccgacga ggccgcagcc aagctgccga
     7561 gcgcgtacta cctcggtcgc gatctgctcg acctcggtga agcgcacctc ggtggccgcg
     7621 tccacgcgca cccgacgtcg acgagcctcg gtcgcgcagt cgagctcatc gccgatggcg
     7681 accacacccg cgcgcaggtg ctgctcgcgc gctgcgccga ctccgcggac gtcgccgcgg
     7741 caaccggcgc atcgctgctc gcgcaacaac gcattgccga cgccctgccc cacttgctgc
     7801 gcgccgccga gctcgagccc gagttcccgc tgcatcactg gaacctcgcc tcggtgctgc
     7861 atcacctcgg cgaccccggc agcatggccg cgtgctacgc cgcgctgcgc cgctttctgc
     7921 gcgcgagcga gcagccgacg ggcctgttcg ccgatccgga tcagcctgcg cgcgtcgggc
     7981 tcgcgattcg cctcatcgcc gatctcgagc gcacagcgcg cctcaccggc ctgaagctcg
     8041 acaccggcac acgccgcaag cgtcgcgcac cgaagcgtga agccgcgaag cgttgattac
     8101 ttcttcgccg cgacggacat cgagtacgac atcttgatgc gcgtcgagcc tgcctgcgcg
     8161 cgcttcggaa gcttccacgc cttcgcgtgc gtctcgacgc aaccggcgac catcgacatg
     8221 ccttcggtgc cggccttcgg ctccgtcgac gcgcccttga tcgcgcccgt ttgatcggac
     8281 tcgaggttga gcgtcacggt gcccgacagc ttcgggtttg ccgcgaggcc gcgctcgaag
     8341 cacgcctgga tctcttggtt gtggcggttg agcagcgccg cgatcgcgtc cttgctgtgc
     8401 gtgccgacgc cggccttcat cgccttgaac gaacgctcga gcgcgtcggc gttcttctgc
     8461 gcatcgccgc gttgcttgtc gtcggtgagc ttgctgaccg tcgcctgata gagatcgtgc
     8521 gcgggctggt agtagcgatc gtcatgcgca gtcgcgtaca gcacgtggaa cagctggccc
     8581 atcaagtaga cggagtagac gatcgtcgcc atgtcctgcg gcgggcactt cgcaccacac
     8641 gcggggagcg cgtcgaccgc tagcttgccg aacttcgccg acgcgaccgg atcgtcgagg
     8701 cgaaccgtgt agtcggcctg ttcgaaacgc agcaccggca tgtcattgac cggcaccttg
     8761 ccgcccgatg cagtgaccgc cttctcgagc accatcacgc cgtcctgcca acgacccgcg
     8821 tactgatacg actcgaggcc gagcttcgcg agcagctcgt actgctgcgc cttgccgaac
     8881 acaggctgca gcgatgcaac cgcttcgtcg atcgacgcgc cggtacgacc tgcgaacagc
     8941 agcatgtcgc gcagcagcgg atcgtgcggg aacagcgagg tgccgccctt cgcggcgagc
     9001 gagatcgcct gccacgcgcc cttgtcgtcg ccggtgcgga acttcgccca cgccatcacg
     9061 taggcgagct cgggagtctt gtcggtgatc tgctcgttcg cgatcacgtt gagcgcgtcg
     9121 gcgctcttgt actgacgcag gagcgcgtac gcccaccacg ccttgaacgt cggcagctct
     9181 ttgtccttcg gatcggacgc gacgagcgcg gaccacgcct tctcggcgcc tgcgtaatcc
     9241 tcgaggagaa gctcgaagct gccgagcatg cgcaggtccg tcggatctcc tttgccttcg
     9301 cctgcagccg tgacggaatc acgcagcacg ccgcgcgcct ccgaccacat cttctgcttc
     9361 tcggcttcgt ccttggcttt cttcgcgtcg tcgtagatca gcgtcgcgag gaccgcggct
     9421 tgcgcttggc gctgcacgac gtccttgccc ttcgccgcct tgtcgtacgt ctggcgctgc
     9481 ttgtccgtcg tcgtcttctt cttcgggtac gcgagcggca tgcccgggct gccgagtgct
     9541 tcgggctcga acaggacgcc gttgacctgg atcgtcggca cttcgaacga gacatcaccg
     9601 gggcccgacg gcttcgcgcc accgccacca ccaccgccgc cgccaccacc cgacggattc
     9661 tcgggtccct tggtcgatcc gccacaagcg acgagaccgg cagtcagcac gaacgcaagc
     9721 gagttacgca tgcgcgaggt gtacaccaaa gccacgcgcg cttgccatgg tgctatggtc
     9781 gcgtgcagtg cgaaggctga gctttgtcgt ggtcgtactc acggcccttg ctagtaatgg
     9841 ctgcgcggtc gtgaaggcac accagcgagg gaacctcgcg aagcgatcga tgacgaacga
     9901 tcgcgatccc ggtgaggcgc ggtttggcca gcacgagcgc ggatcgcgcg aaggcgccga
     9961 cggcggcacc ggccaaccgg gtggcggatg cggctgcaac tgatcgcagt tgtcctcgtc
    10021 gtcgcggcgg gaaccgcgta cgccgatggc acgctgtcga tgcgcggcgt ctactacaaa
    10081 gagcgctcga cgcgcgtcgt ccagccgatg ctcgacgcga tgttcgaggt cggtgcacac
    10141 ggcttggcgt ccggccatct cgccgtcgac gcgatcacgt ctgcttcgcc gagctcgggc
    10201 gccgaagccg aggcattcac cgagcagcgc tacgaaggcg gcgccggcta cgcgcacgag
    10261 gtcgagggcc ccgacgattc gtggatcgac gtggtgcgcg tcggcggcga agcgaaggtc
    10321 agcaaggagc ccgactaccg atcgatttac gcgggcgcgc gcctcgaagc agagctcgcg
    10381 caaaagaacg cgacgctcgg cctcggcggt ggcgtctcgc tcgacgagat caacaacacc
    10441 ggcgcgcaga gcccgatggg tggccccaag ctgctctgcg acaacaagct cacgatggcg
    10501 accgagacga cgtgcccgct gaacacgtac tcggtgttca cgtccgcgag ccagctcgtc
    10561 gacaagaacg cgctcatcgg cgtctcgtac gatctagcga aggcgcacgg cttcacgtcg
    10621 aacgcgtatc gccaggtcat cacgacgagc ggctacgtcc cggagaagca cccaaacgat
    10681 cggcttcgcc aagcggtcgc gatctccggg cgctactact ggcctcgcgc gaagaccgcg
    10741 ttcatcgccg cgtatcggtt ctactgggac gactggaaga tccacgccca cacacccgag
    10801 ctgcggatca tccaggaggt cggcacgacc gtcgatgcgt cgttccgcta tcgctattac
    10861 aaacagaatg cggcgttctt ctacgagaag ccgtacccgg atcagagcgt gcagatgtca
    10921 cagtatctga ccgacgatcc gaagatgacc gcgttcgatg gtcacctgat ggaggcaaag
    10981 ctcggcatcc tcggcgagac gttcggcctc aatggacgct gggcaggcgc ccgaatcgag
    11041 ggcatcctcg aatacatcgt tcagaacaac cgattcggaa atgcggtggt tgctcatgca
    11101 tcgcttactg ttcctttcga ctattagtgt cgccgctctc gtcggctgcg aaggttgcgg
    11161 cgacgacagc tgtggtcccg gtgacgcgcc cgcgacgggc ctcatcgtcg gcgatcaaga
    11221 tcagaagctg acgttcggca atttgtcgtc gagcgccaac aacgactgcc ccgatccgag
    11281 cgcgcccgcg ggcgttgtca gcctcacggt cagcgggtcg cagacagatg cgccgaccgg
    11341 tgggtccggg ctgctcacgc tctgcatccc gcgcccggat cagctcgcgg gcaccgtgca
    11401 gctcggtacc ggcgtgaaga tcatcgatct gcacggccaa gcgaacggct gcatgtactc
    11461 gatcgaggcg acgcgcccga tcaccggcac ggcgtacggc agcggcgtct gcaacaacgg
    11521 tcaggacggc gccggttacg cgctcacgtt cgacggccac atctcactga agcgtgagtg
    11581 cccgacggcg accgatacga tcgcggtgaa cgtcagcggc gaagtcgccg tcgccgggca
    11641 ctaaacgctg agcttgcgaa ccgcgtcgac gatcggcgcg agcatcgcgc gcaccatcgt
    11701 gcgcagcaga tcagactcgc cgatcatccc ggcgaccggc gggccgaacg tgtaatacgc
    11761 ctcgacggcg agctcgccga gcaccgtgct ctcgagcagc gaatcgcgga agtgacgcag
    11821 cagctcgacg tcgttcgcca tcaccgagcc gtacgccgca gtcgcgatga agcacgcatc
    11881 gacttcgcct gcgatgcggt ccgcggtccg gacattcacg atcgcgatgt cgccgttgtt
    11941 gcggcacgcg tcgaacgcgc gaatcgcgac ggagtagtcg gtctcgggca gaaggcccat
    12001 gagctcgagc gtctgcgtct gcccaggccg gacgagcgtc gcagcgacgc cgaccttcat
    12061 cgcgtcgtcg aacgtctccg gcgtgatgac gccattggct ttgatgcgga cgtcgtagcc
    12121 agccacggtg ctcgtgccat caccgggcgc cgtgaatgcc agcgtgatcg tctgcgtgcc
    12181 gagctcggcc gccttgatgt tcgccggcgc gctcggaaca tgcgagctgt cgttcgggct
    12241 gaccgagacg cgcacgcggt acatcgcgct gccgtcactg acgagctgca agcgggacgc
    12301 gcccgagccc ggcgtgtcgg tcgtgatcga gccgtccggt gggcgaatgt tgccgtcgct
    12361 gccgtcgatc gcgctgtagc cgatgaagtc actcgtcgac gctgtcgtct gccccgtcgt
    12421 gatctcgaac ggcactttgt agacgacgga cggctggccc aggtacggca acccgaacga
    12481 cgaccactgg atccccatcg gcccgaccgc gggcggcgcc gggtgcgcag cttcggagaa
    12541 cgtcgcgttg tgatcgaact ccttcgcgac ctcgacgaac atcacgtacg agcctgccgg
    12601 caacgcgtcg ggaaccggcc atgcgatcgc ctgcgcaacg ccacccggcg gcgtggcctg
    12661 cgagaccgcg tcgaacggcg ccatcgtctt gaactgttcg accgacggcg cgtcggtctg
    12721 cgggttgtag atgagatcga gtcgcggcgg atagaggctc gtcgacgtcg tcgagaattt
    12781 gcccttgtcc gtatgcgcga ccgtcgcgca cgtcatcgcg tcggccttcg cccacttcgg
    12841 atccgacgcc atgaacggct ggcagtagtg cagctctcgc gagctctcgt cgaacgcatg
    12901 gctgaggttg ttctcgccgc aatcggcgaa ctctcgatcg tttggcgagg acttcgtcga
    12961 atcgttgatg acgctgaagc agtagttcgg atcgtcggtc accggaacgc tgccgcccgt
    13021 cgagcccgcc atgtggccgc agcagttctg gaacaccacc gacggaaacg tgtggccgtg
    13081 gcgatgcgcc cacaccggaa acgtcgcgag ccggcggcca tacggccaca tcggcccgga
    13141 gttgaagtcc cagcgccccg ggcgattgcc aaggccgaag cgaccgaccg tctgggtgat
    13201 gtacgtcgtg tcgacgtact cgccgctcgc ggtctcgatc cacgcgacga tctgcagctt
    13261 ctcgctcggc gtcatctcga tgctcgcgca gcgagtctcc gcgtgcgccg cagtgggaaa
    13321 cgcgagagcc atcacgaaca ccagccgctt catcgcttca cccactcgac gatcggctgc
    13381 agcatgccgc gcgccagctg gcggagcaca tcggactgac cgacgacacc cgcgaccggc
    13441 gggccgaacg tgtaatacgt ctcgatcgcg agctcgccga gcacactcga gcgcaacgtc
    13501 gcgtcgcgga agtcgcgcag cagctcgacg tcgttcgcca tcatcgagcc ataggcagcg
    13561 gtcgcaatga agcacgcgtc gacggtcccg acgggacgat cggccgtgcg cacgctgatg
    13621 accgtcagcg gaccgctgtt gaagcaatcg tcgagcgcgc gaacaccgat ggagtactcg
    13681 gtctccggca agaggcccac gagctcgatc gactggagct cgcccggatt accgatggcg
    13741 atcgacgtcg tcgcgcgtgt cgccgtctcg aagttctcag ccgtgagctc ctggttgacg
    13801 aggtagcgca cttcgtacgc ggagaccttg ccgacttgcc cgtcgtcacc gggcgcggcg
    13861 aaccgcagcg atgcagtcgt cggcgtgaca gccacgacct cgccagcttc cggcgcaccc
    13921 ggcgccgcgt aatcgaactc gggacgcgcg accacgcgca gccgatacag cgcgccaccc
    13981 tcggagatga gctgcaagcg cgaggcaccc gagccgggcg tgtcggtcgt gatcgtcgag
    14041 tcgggtgcgc gcacattgcc atcggtgccg tcgggcgcgc cgtaaccgac gtacgcgtcc
    14101 gtcgtcacgg tcagctgctc ggtaccgagc gtgaacggaa ccttgtagac gatcgacggt
    14161 tggcctcgat acggcaagcc gtactccgcc cacgtgatcg cctgggagcc cgtgttcgcc
    14221 ggcggcggct gcgtcgtcgg gttgtacgtc gtgttgtgat cgaacgcctg cgagacttcc
    14281 gcccacacga cgtagtcgcc ggccggcgtg ttcgcgggca gcggccacgt caccgtctct
    14341 cgcgtgcccg gcatcggcgt cgcgcgtgat accgcgtcga atccggtgag ctgcttgtac
    14401 atatccaccg acggagaatc gacgttcgtc ttcacgacat cgagccgcgg cggatagagg
    14461 ctcgtgcgca atgtcgacag cttgcccttg tcggtgaacg cgggcgacga gcagctcatc
    14521 gcgtcggcgg cctgccactt caccgcgtcg ttcggatcgg cgaggttgaa cggccggcag
    14581 tagtgcgtct cgagcgagct atcgcagctg tagtggctca ggttgttgtc gccgcagaac
    14641 atgaagtcca tgcccatgag gccgtcgcac gtatcctcga acgtgcacga gtcggtgcag
    14701 aactccggcg tctgatgtgc gccgttgatg cagtcgtcgt agcaacagtc gtcgaagcct
    14761 tccatgttct ggaacacgac ctcgggatag cgctcgggct tgcgatgcgc ccataccgga
    14821 aacgtcgtga tgcgccggcc gtacggccac atcggtccgg acttgaagtc gtagcgaccg
    14881 gggcgattgc caagcccgaa gcgaccggtc tgcgcggtga tgtacagcgt gctgacgtag
    14941 tggccatcgg cggtctcgag ccagacgacc atctgcgacg gctcgtgcct gctcgactcg
    15001 ctgctcgtgt tctccgccgg cgtgaagtcg acgtcgatga cgtggcactc gtggacttcg
    15061 gcgtgcgccg tgccgaccag cgccgtgaac gcgccgatcg ccgtccatcg cgccgccggc
    15121 atcgctctca tcttagtacg acagcttgcg gaccgtatcg acgatcggca cgaggaagcc
    15181 gcgcacgctc gcgcgcagca ggtccgactc tccgatgacg cccgcgaccg cagggccgaa
    15241 cgtgtaatac gtctcgatcg ctagctcgcc gagcacgctg cgaccgagga cgccatctcg
    15301 gaatcggcga agcatcgcga cgtcgttcgc cattaccgag ccatacgccg cagtcgcgat
    15361 aaagcaccac ggcacttcgg gcgcctcgcg atagttcgtc ttgaacgtcg tcgtcgcgag
    15421 cgggccgcgg ttgaagcagt tgtcgtaggc cttcacgccg acgaagtact cggtctccgg
    15481 aagcaggcca gagatcgtga ccatctgcat ctcgccggga gatgtcggca cgagagtggc
    15541 cgtgaccggc atgctctcct tgaagttctc gtcggtgacg ggcgagccga cgcggacgcg
    15601 cacttcgtag ccggtgactg cgccgatgct gccgtcgtcg ccgggtgcga ggaactgcag
    15661 cgtaccggac gtgctcgtga tgccgacggc ttcgatcgat ccgacttcgg cgggtggcgt
    15721 cgagtcgaat tcgggcgtcg cacgcacgcg cacgcgatac atctcggcgc cgtcggacac
    15781 gagctggaat cgcgatgcac ccgagcccgg cgtcgtcgtc gtgatcgtcg cgtccggagg
    15841 attgagcgtg ccgttcttgc cgtcgggatc gccgtagccg acgtagtccg tcgtcgtcgc
    15901 gcgcgtgggc gtcgacgcga tcgagatcgg aacggagtag acgaccgacg gctggcctcg
    15961 ccacgccttg ccgtactcct tccacgggat gtccgtaagc ggcggatacg tggtctcgtt
    16021 gtacgtcgcg ttgaagtcgt acgtcttcga ggcctcgacg tagagcacgt agtcgccgta
    16081 gtcgaccgac tcgggcgctg accacaccgc ggtcgccgag agcccgccaa acggcgtcgc
    16141 ctgcgagacc gcgtcgaatg gattgttcgc gcgaaacata tcgacggccg gtgagtcgct
    16201 actcgggcgt cgctcgatgt cggagcgcgg tggataaagc gagaacgtcg agccgtcatc
    16261 agtctcgctc gagccgatcg cgaacctgcc cttgtcggtg aacgacgcgg atgcggtggt
    16321 ccctgcatcg aagccgttct cgttcggctg gatcggccga ccgtacggcg gcggcagctc
    16381 gggcgagctc tgcgcaaacg gatgcgagag gttccgatga tcgaggttct ggaagatgac
    16441 gaccgggaac tggtgcccgt ggcggtgcgc ccagaccggg aacgtctgca cgcgacggcc
    16501 atacggccac gtgtcgttcg acgcggatcc ggtgttgaag ttcgggatgc ccgggcgatt
    16561 gccgaggccg aacctgccgg tcttgttcgt gatgtacgcc gtgtcgacgt acgtgccgtc
    16621 ggttttttcg agccacgcga cgatctggag gttgtcggtc ggcgtgaaat cgaccgccac
    16681 cgtcacgcag acaccttgcg gtcggtcggc cagcgcagtt gccgggatca gcccgagcag
    16741 caacgggagc caacaccgaa gcgacatcac tcgcgagttt gtcacacctt cggcctagcg
    16801 tcgtgtcgtg gcacgcacgc cgagcgccga atatctgatc ctcgacatcg agaccattcc
    16861 cgatgtggag cgctggaagc ggccggacct tccgagcgtg acggcggaca cgcaggctcc
    16921 cgcagacgct gcggcacccg acgtcgtgcg gcccgcggaa ccaccacgcg ctggtcgcgg
    16981 cgatcgcggc ggatttccgc cgtcgtgggc gcatcgcatc atcgtcattg gatgtctctg
    17041 gctagatcac agttacaggc tcaagcgcct cggcgtcgtc ggtgatccta cgggtgggga
    17101 ggggtcaccg gatcaccgcg aacgcgtgct gctcgaagat ttttcgagat ttgtcggccg
    17161 cgcccggccg gtgctcgtca cctacaacgg acgcagcttc gatctacctg tgatcgcatt
    17221 gcgcgcgttg tgtcacggcg tgtcgatcgg ctggtactac cgcgagcgcg acgtccgcta
    17281 ccgctacagc gagcaggggc atctcgatct gtgcgactgg ctcgcggatc acggcgcgac
    17341 gcggtcgggc agcctcgatg ccgtcgcgcg gttgatcggc ctccccggca aggtcggcgt
    17401 cgatggctcg caggtcgagg ggctctatca atcggggcag ctcgcatcca tccagcgcta
    17461 ctgcctcgcc gatgtcgcgc agaccgcgct gctgtttctg cgatttcgtc tgctccaagg
    17521 cgtgctgcgc agcgatcagt atcaagacgc gacgaacgca ttgctcgacg cactcgccgc
    17581 ggatggtagg gtcaacgacg tgctcgaggg taccgatcgc gcgcacctgc tcggcccgac
    17641 acccaccgcc tgatgccagc gaagtcagcg aagtccgaga tcgcggagct cgcgaagcgc
    17701 cgcttgccgc cagcgtggtt tcaacgtgac gtcgtcatcg tcgcgaagga gctcgtcggt
    17761 tgcgcgctcg tgcatcgcaa tcgcgcgggc ctcatcgtcg agaccgaggc gtacatgggc
    17821 cccgacgatc tcgcgagtca cgcgcgcttc gggccgaccg ctcgcacgtc ggtcatgttc
    17881 ggaccgggcg gcgtgagcta cgtgtacctc tgctacggca tccatcagat gttcaacatc
    17941 gtgacgggca ctgagggtca gggacaggca gtgctcatcc gcgcggtcgc tccgctcgtc
    18001 ggtctaccgg acgatccatc cgtcggccgc ggccccggca aggtgaccac cgcgctgaag
    18061 ctcgaccgcc ggcacgatcg caaagacctc gcgagtcatt cgcgcttcgg gccgaccgca
    18121 cgcacgagcg tgatgtttgg accgggcggc gtgagctacg tgtacctctg ctacggcatc
    18181 catcagatgt tcaacatcgt gaccggcgcc gccggtgacg gtcaggcggt gctgatcagg
    18241 gcggtcgccc cgctcgtcgg attgccggac gatccatcga tcggtcgcgg tccaggcaag
    18301 gtgaccaagg cgctcgcgct cgatcgcagc cacgaccgca aggaccttgc cgacggcacg
    18361 ctctacgtcg cgcgctatct ggcgccgccg cgagtcgcca agggaccacg cgtcggcgtc
    18421 gcgtatgccg gtccgtgggc ggagcgcccc tatcgattct ggtggcgcga tcaccccgcc
    18481 gtgtcgcgcg tgcgatgaac cgtggcccct aggcgcgatg ggtcgcgacc gaatcgacct
    18541 tgcgcatccg ctggcgatag agctcctcgc cgagcttttt gcgatcgcgg ctcttcgccg
    18601 gacggtagtg aaaatgcgcg ccctcgcgca cggtgaccag cgactccgcg ggcggcacac
    18661 cgaggtcctt gagccagccg cacgcttcga cctcgagcgc gcgaccctcg gcgagccaga
    18721 gcaccgtctt gtgctccgac agccgcagcg ttgcgttctg cgcgcgcacg tagatcgaca
    18781 gctcgaggtg gccattgccg atcagctcgg tgtcgtgatc gtcgatgaac cggatgatct
    18841 tcttcgcggt cacccgtgag gtcagccaaa cgtcggtggt gcccgcatcc tcgaagtgat
    18901 cgagcacgcg gacctcgagg ttgtcacccg cgggcaggcg ctcgaccatg ccgagaaaca
    18961 ggtcggtcag tttgtcgtcg gtggtctgcg cttcgatcac gaggagcttg gactcggggc
    19021 gcatcaccca gccgggagca gcgccggcga aggccgatcc tggtgcggcg agggatggca
    19081 gaactcccgt cggccactcg aacgtcggcg tgtcggcggg cgcatcgccc agcatcagca
    19141 cgtgaccttt gccgacggac tcgccgagcg gaatgtcctg gccctgtgca tgctcgacgg
    19201 caaccggcca gcttccggca acgtgatcgc gcgcttccgc gatcgcttgc cccatgtgct
    19261 cgccgctcgc ggcaaccagc tgggtcggcg ctcccgcacg agcgagggcg atgcggtacc
    19321 aggtgcgagt gaccgctttt gtcacgtgtg ggttctaccg cggatcggtt gcgcgagcgc
    19381 cgcacgtggg cgcacgatgc cgcccaatga acaagctctc aatgtttgcg tgcggcatcg
    19441 tgctcgccat cggttgtggt tcgaaggatc cggtcgacaa ggcgatcgcc ggtctcgagg
    19501 actggaaggc aaagatgtgc gcgtgcaagg acaaggcatg cgccgacaag gtccacgagg
    19561 actacaagaa gtgggagaac gacgtcatgg agaagtcgct caaggacgtc gacaagaaga
    19621 gcgtctccaa ggacaagatg gagaagttcg ataagctcga tgacgcgcgc aaggattgcc
    19681 gccgcaagtt caacgagccc gaggccccgc cggccactcc gcccacgccg taatcagagc
    19741 ggttcgagct ccctgtactg gagcaccacg atcgtgactt ttccttcgac gacgcctccg
    19801 atggtgtcgg cacgctgagg tgacgtcggc agcgagaact gcgtcccgaa cggtgcatcc
    19861 gtcgagacga agattccgct ggccgacgtg acgccgccgg tgatcacgcc ggtccttccg
    19921 ttattgagat actgcagcgt gcagccagcg gggcacgaca ccggctcgcc ggcgcgcgga
    19981 ttgaaattct cgtcgacgac catgccgacg accagtccgc cctgggcggg aaactcggtc
    20041 atgccgatcg cgctgaggat ctgggcaagc gtcgccttgc gcaggtagta gccgagcaga
    20101 ctgtcgtagt cgttctcggt caccgccttg gcgttgcacg tgagcgcggt cgtggtctgc
    20161 gccccatcct cgcgtaccgc gatgcacgcg atgttcatga acttgcgttc gatcagcgcg
    20221 ccccacgacg gcgtcgatgc ggtgctgcgc tcgagtgggt cactctcttc gggaccgagc
    20281 acgtaggtgt cggcgaacgg tttcggctcg ccgatgtcga cattgagccg gtcggcgacg
    20341 gtcttgtcga ccgacgccgc atcgtcgaag tcattgacgg tcgcgttgac cgtgacgcga
    20401 ttttcgccga tgcagaccgc cgcgtcggtc aattgctcga ggtttgtaca accgaggtcg
    20461 acgaccgtct cgtcatcgcc ggggtggtag tacgtgcggc caccgacggc aggcacgcgc
    20521 gcacatgcga acggatccgg atcgtcacag atcggcaaca cgggcatcgg caggccgttc
    20581 gcagcgaact gcacacgcgg gcacaccggc tccccggtca cgggatcggg ttggatatcc
    20641 attgctcgaa acaccgcc
//
LOCUS       DATLBZ010000085        11098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5290460, whole genome shotgun sequence.
ACCESSION   DATLBZ010000085 DATLBZ010000000
VERSION     DATLBZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5290460"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3702)
                     /locus_tag="VMZ53_04555"
     CDS             complement(<1..3702)
                     /locus_tag="VMZ53_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27749.1"
                     /translation="MLEGIAATKRLPFTFHAHGGVGRLVLADLNVPPLHVERLELEVT
                     DLGTDPGATAAERFQRRRTRLRALTARVTSHAIDARVETVRKPLAGLGITNVSARLND
                     GYVSFRARAADGLAAADLSFRVHLVTSGTQLRALASHIRVHGHLPTPGPIVADRILTA
                     LLGASDAQGVVERPHVRGLCDVEIDLVGALLWHLMPPAGWRLPAIGEIDLVHVRISRA
                     AIDVAYGPSGSRTGDLGVRPAPLALAAAHDLMHSADQQLRTGHVEEAMRGYRALLAAG
                     GPEQPQLLERILALAAARPAWFFDGLELARQALGRWPSFPAAHATLTSIMLAQGDARE
                     AASHLAQLAKQASADGDDDQAALAALAGARLLRVLEPKAATQLYELALEHDPGSAEAA
                     DALADRLADEQRWPELVRLLRARAVAAEPARAVELRLRLADVFVHQLGDPSSAQHELA
                     AAREVAPDEPAVHEMTATILASSNPAAALDAWRNVARLAQGRGDTRSAAKAYAIVGEL
                     LAKRYAAADAEAWEEAERAWQKALELDPLQTDAIAGLATAAAARGAHGAAADLYERLR
                     GLGLPQHTSARYELMLARSLVHLGRIDEARASLRRATLAGGETAAEAHAVLAEIAQAA
                     SDREHAAAELDTAITSFVGLAGEGVTTDERMLTRAAELAIARASLLDSAGHANQAMAD
                     YERAHQLAADHAPQLARDAARTMLAKAADAQSERRWIDAVLATRPPPMERANLLVHRA
                     DVRRRERVPDIAAAVADLHEALSVIENSDESSAANELRRRAYQLEADLLAASGDQRAR
                     AKALTALARLAERSPERSADRVEVETAAAAAWLAANEPATALPHGARAHAELVADISP
                     NLRREVLTTLGEAAWRQRAWPDVIRAYRGLVDDTSVDPERVGVYRYRLAVAGDRSGDV
                     KLAMEALRPLVDYLSDPTTPGADAARATPPEVRGQALRLFADLAERAGDLGGAAAALE
                     GFAGVALESTPSARADAMYRAGELFRRADRGDDAVRCLEAALRISDTHLPALDALELA
                     WRERGDIERVSVILGRKVAATARHPHRQKPLLSRLGDLQDQLGRPDVALATHQRALEI
                     DPAWRPSLRYVTSRLSADGAVVAAAGGFAQLAGELTSESGVDAAIVTRDRQLAAQALA
                     ELVTKLDDAQVEAVRSVAESAVQRAARDGIANIEPALARLRGDAPVPLASRTFSAEEN
                     TPSGRMKDAPQGALSLRDAAKRSRAAGKLA"
     gene            complement(3767..4849)
                     /locus_tag="VMZ53_04560"
     CDS             complement(3767..4849)
                     /locus_tag="VMZ53_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS27750.1"
                     /translation="MTIDAYEHAACGLMQTADDGLVRRVNGTFCAWLGYTADELVGKR
                     RFQDLLGVGSRIFHHTHWLPLLAMQGSVSEVKLDFVHRDGAMIPMVVNVVRRDDEGTI
                     VLDVAVFIGRDRDRYEQELVLAKQRLESLVTEQRDRADFAEQLIGIVSHDLRNPLSTI
                     GMASQMLANKDPNAQDRLVAMIDRSVDRATHLLADLLDFTAARVGNGISISVTNVDLH
                     STVAEAVDALHVAYPKSTLTHVTQGSGTCRADPQRISQLLGNLVSNAVSYGNPTRPIE
                     IASRIDDVACALSVTNAGPAIPEDQLASIFLPMTRGTSKGTASRSVGLGLFIVQQIAK
                     AHGGEARVASTHEATTFTIELPRKTA"
     gene            complement(4846..5658)
                     /locus_tag="VMZ53_04565"
     CDS             complement(4846..5658)
                     /locus_tag="VMZ53_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS27751.1"
                     /translation="MDLHKKLNVHVKGDGPATMFFAHGFGCDQNMWRFIAPHYAKSYR
                     VVLFDLVGSGGSDLSAYDRKKYASLDGYAADVVDLVVELSKGPALFVGHSVSAMIGLL
                     ANLAAPERFGAQIMIGPSPCYINDGDYVGGFSRGDIEGLLATLEGNYLGWASSMAPAI
                     MGAPDKPELGVELTNSFCRTDPEIAKQFARATFLSDNRADLARLTTPTLLVQCDDDLI
                     APKSVGDYLHKHIPNSTLTIIKNLGHCPHLSAPSATMEAIDAFLAKERFGAS"
     gene            complement(5728..7881)
                     /locus_tag="VMZ53_04570"
     CDS             complement(5728..7881)
                     /locus_tag="VMZ53_04570"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HUS27752.1"
                     /translation="MLENARKQGASIFVYLIFCLLIAIFVINFGPQGGGREGGCTGSD
                     NTVVRVDGKDITPTAYHVAYALSSAPGKRKTHGAIEMLIRREILAGEAEKHGISVNED
                     LVQEEIKKGHFFYAGREETIPQIFDEDGLWRLQAFKNWIGQLNVTRKAYVSEQQRALQ
                     AALMAKILVDSVQVSRDEALQHFLFEGNTVLYDVVAFRPENYKTPMKLTDADVDRFIS
                     THGAEVEARFKADERTYKAVKPQLKLRQIFIAKLEEPAAPTPAPDQGSAAGSAAGSAA
                     GSAAGSAAPPAAAPPAPKPEAKAVGMKIEEAKAKLEAARAAIAAGKQKFADAAKQLST
                     DENFKANGGELGWRTADNASLGDKAASDAVKDLKAGEMTPVITTDRGAWLVIAEEKRE
                     GDLSYDQVKHEIAKELARDVWAKEAAKRAALLALDKAHAGVGLNLDQMYEKEKAPEQP
                     GIDIQQILNDPNMSDEQKQKVLQMLLQQQKSGSYVVEGKDIPAAWYAQADGSSAGSAK
                     PAAATGSAGSAATKPATPPAAGSAATKPATPPAAGSAAGSGAGSAASAPTATPAAGAP
                     VPPPADLTPSTDKLPEFGTVEPPHVARFGPAPRTPTMSGIGSSKEAITALFDELTPGM
                     LAKRVYEGDGAYYVMQLITRADPKVEEFDKEADRLVEELRQQRAQAFLEGWMKERCET
                     LAKDGRISVNPALIRETDDTGKVLPTSYKVCMSFR"
     gene            8080..9114
                     /locus_tag="VMZ53_04575"
     CDS             8080..9114
                     /locus_tag="VMZ53_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HUS27753.1"
                     /translation="MLFDWVYGMFSNDLAIDLGTATTLTFVKGRGIVANEPSVVAVQA
                     VSQGPKKVLAVGKEAKEMLGRTPGNIVAIRPMKDGVIADFEVTEAMLRYFIQKAHSRR
                     TLVRPRVIVSVPSGITEVEKRAVRDSALAAAAREVYLIEEPMAAAIGGGLPITEPTGN
                     MIVDIGGGTTEVAVIALAGVVLSKSIRVAGDKMDEAIVQYIKRKYNLLIGERTAEIIK
                     KEIGSAYPLEEPMTVEVKGRDLVAGVPKTLLVNSDEIREALTEPVNAIVDAVRSVLER
                     TPPELSADIVDKGIVLSGGGAQLKNIDVLLREETGLPVMVCENPQLAVVLGTGKVLDE
                     LSLLREIALR"
     gene            9129..10046
                     /gene="mreC"
                     /locus_tag="VMZ53_04580"
     CDS             9129..10046
                     /gene="mreC"
                     /locus_tag="VMZ53_04580"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HUS27754.1"
                     /translation="MQTTLKRRLIDWGATALLVLIPVLVLRASLHRSEPSGLDKALLR
                     ITAPLQAGVSWVVEGIGGLWGRYVAVVDVEDENRELRAENERLRRELSTMTRRAYDIQ
                     ALEDLAVVKRSTPADTLGARVIGAPMSPQFRVLRLRIDRGDKDVQIGMPVITGTGPVG
                     RINKVFGDYADVTLISDPSSSVEVVIPRTGGRGILRGLGRNDSYTCAIEWLERSKALQ
                     TPAESRVQLGDEVVTSGLGASFPPGLVIGKVSSVGKDDGMFQKVEVDPAVDVSRVRAV
                     EVLLAPPPPPDPDAKTKRKSEPAFGGRPL"
     gene            10043..10171
                     /locus_tag="VMZ53_04585"
     CDS             10043..10171
                     /locus_tag="VMZ53_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27755.1"
                     /translation="MITLLIMAMVLLAMLAVEAPLWMVVAILIAWVLVAGFGRKKR"
     gene            10168..10710
                     /locus_tag="VMZ53_04590"
     CDS             10168..10710
                     /locus_tag="VMZ53_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27756.1"
                     /translation="MRTATIVLVAYVVCVIVAAIWRYMPGFTRDAVPAIGALTAAYLG
                     HTTRPYRSSSIAGGVVLGYLIDVISGTPPGLSSIALAMTAMVARGTQQRIFVRGAAMT
                     IAYSAFIALFASLARLVTMVLFGVPRAPSFALELKFMALTAFATALVGPIVWRLFRRI
                     DAAYARTHRERDAALEGLAP"
     gene            10707..>11098
                     /locus_tag="VMZ53_04595"
     CDS             10707..>11098
                     /locus_tag="VMZ53_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27757.1"
                     /translation="MMYRHQIQSSNGGVGLPELHKRVKWLAIIVIAAFALLIGRLWQL
                     QVIRGDSYYERTVSNVVKERYLPSVRGKILDSKGIPLADNRPAFNIYATPKQLTPDAK
                     AELVRMLGLSDDEIGKLDERIAVGVKRDA"
BASE COUNT         1730 a         3661 c         3771 g         1936 t
ORIGIN      
        1 ggcgagcttg ccagctgcac gtgagcgctt cgctgcatcg cgcagtgaca gcgcgccttg
       61 tggtgcgtcc ttcatgcgcc cgctcggcgt gttctcctcg gcggagaacg tgcgagatgc
      121 aagcggcacc ggcgcgtcac cgcgcaggcg agcgagagcg ggctcgatgt tggcgatccc
      181 atcgcgcgcc gcgcgctgca ccgcggactc ggcaacactg cggacggcct cgacctgcgc
      241 atcgtcgagc tttgtgacga gctcggccag cgcttgtgcc gcgagctggc gatcgcgtgt
      301 gacgatcgcg gcatcgacgc cgctctcgct cgtgagctcg cctgcgagct gcgcgaagcc
      361 acccgcagcg gcgacgaccg cgccgtcggc cgacagcctc gacgtcacgt accgcagcga
      421 tggtcgccat gcgggatcga tctcgagggc gcgttgatgc gttgcgagtg caacgtcagg
      481 acggccgagc tgatcctgca gatcgccgag gcgcgagagc agcggctttt ggcgatgtgg
      541 atgacgcgcc gtcgccgcga ccttgcggcc gaggatgacc gacacgcgct cgatatcacc
      601 gcgttcgcgc catgcgagct cgagggcatc gagggcggga aggtgcgtat ccgagatgcg
      661 cagcgctgct tcgagacagc gcacggcgtc gtcaccgcga tcggcgcgac gaaacaattc
      721 gcccgcgcgg tacatcgcat cggctcgtgc gctcggcgtc gattcgagcg cgacgcccgc
      781 gaaaccttcg agcgccgccg cggctcctcc gaggtcgcct gcgcgctcgg cgagatccgc
      841 gaacaatcgc agcgcctgac cgcgcacctc gggtggcgtc gcacgtgctg catccgcacc
      901 gggcgtcgtc gggtcggaga ggtagtcgac gagaggacgc agcgcctcca tcgccagctt
      961 cacatcgccg ctgcgatctc ccgccacggc aaggcgatag cggtacacac cgacgcgttc
     1021 tggatcgacg ctcgtgtcgt cgacgagacc gcgatatgcg cgaatcacat ccggccatgc
     1081 gcgctgccgc cacgccgctt cgccgagcgt cgtgagcacc tcgcggcgca ggttcggcga
     1141 aatatcggcg acgagctcgg cgtgtgcgcg cgcaccgtgg ggcagggcag tcgccggctc
     1201 gttcgcggcg agccacgccg cggctgcggc cgtctcgacc tcgacgcggt cggcagagcg
     1261 ctcgggcgat cgttcggcga gccgcgccag tgcggtcagt gccttcgcgc gtgcgcgttg
     1321 gtcgccactc gcggcgagca ggtctgcctc gagttgatac gcgcggcggc gcagctcgtt
     1381 ggcagcgcta ctctcgtcgc tgttctcgat caccgagagc gcttcgtgca gatccgcaac
     1441 cgccgcggcg atatcgggca cgcgttcgcg gcggcgcaca tcggcgcggt ggacaagcaa
     1501 attcgcgcgc tccatcggcg gtggccgggt ggcgagcacc gcgtcgatcc agcgtcgctc
     1561 gctctgcgcg tctgcagcct tcgcgagcat cgtccgcgcg gcatctcgtg cgagctgcgg
     1621 cgcgtggtcg gctgcgagct gatgcgcgcg ctcgtagtcg gccatggcct gattcgcgtg
     1681 accggcgctg tcgagcaagc tcgccctggc aatcgcaagc tccgcggcgc gcgtcagcat
     1741 gcgctcgtcg gtcgtgacgc cttcgccggc gaggccgacg aacgaggtga tcgcggtatc
     1801 gagctcggcg gccgcatgct cgcgatcgga cgccgcctgg gcgatttctg cgagcaccgc
     1861 gtgtgcttcg gcggccgtct caccgccggc gagcgtcgcg cgccgcagcg aggcacgcgc
     1921 ttcgtcgatg cgcccgagat gaacgagcga gcgcgcgagc atcagctcgt agcgcgccga
     1981 cgtgtgttgc ggtagcccga ggccgcgcag ccgttcgtac agatccgctg ccgccccatg
     2041 ggcaccccgt gctgctgccg cggtcgcgag cccggcgatc gcatcggtct gcaggggatc
     2101 gagctcgagc gccttctgcc acgcgcgctc tgcttcctcc cacgcctctg catccgcggc
     2161 cgcgtagcgc ttcgcgagca gctcgccgac gatcgcatac gccttcgcag cgctgcgcgt
     2221 gtcaccgcgg ccttgtgcga ggcgagcgac gttgcgccac gcgtcgagcg cagcagccgg
     2281 attgctgctc gcgaggatcg ttgcggtcat ctcgtgaacg gcaggctcgt cgggcgcgac
     2341 ctcgcgcgca gcggcaagct cgtgctgcgc actgctcgga tcgccgagct gatgaacgaa
     2401 cacgtccgca agccgaagcc tcagctcgac cgcgcgtgcc ggttctgccg cgaccgctcg
     2461 cgcgcggagg aggcgcacga gctcgggcca gcgctgctcg tcggcgaggc gatcggcgag
     2521 cgcatccgcc gcctccgccg agcccggatc gtgctcgagg gcgagctcgt agagctgcgt
     2581 ggcagccttg ggctcgagca ctcgcagaag tcgcgcgccg gcgagcgcgg cgagggccgc
     2641 ctgatcgtca tcgccgtcgg cgcttgcttg cttcgccagc tgcgcgagat gacttgcggc
     2701 ttcgcgtgca tcgccttgcg cgagcatgat cgacgtcagc gttgcgtgcg cagcggggaa
     2761 gctcggccat cgcccgagcg cttgacgtgc gagctcgagg ccatcgaaga accacgccgg
     2821 tcgtgccgcc gcgagagcga ggatgcgttc gagcaactgc ggctgctcgg gaccaccagc
     2881 cgcgaggagt gcgcgatagc cgcgcatggc ctcttcgaca tgaccggttc gcagctgctg
     2941 atccgccgaa tgcatcaaat catgcgcagc ggcgagcgcg agtggcgcag gcctcacgcc
     3001 caaatcgccg gtccggcttc cggacggtcc atacgcgaca tcgatcgctg cacgcgagat
     3061 gcggacgtgc acgagatcga tctcgccgat ggcaggcaac cgccacccgg ccggcggcat
     3121 gagatgccag agcagcgcgc cgacgagatc gatctcgacg tcgcacagac cgcgcacgtg
     3181 cggcctctcg acaacgcctt gtgcatcgct cgcgcctagc agcgccgtca ggatccgatc
     3241 cgcgacgatc ggtcccggtg tcggcaggtg gccatgcacg cggatgtgac tcgcgagggc
     3301 gcgcagctgc gtgcccgacg tcacgaggtg aacgcggaac gacaggtccg ccgcggcgag
     3361 gccatcggcc gcgcgtgcac ggaacgatac gtacccgtcg ttcaggcgcg cggagacgtt
     3421 cgtgatgccg aggccggcga gcggcttgcg caccgtttcg acgcgcgcgt cgatcgcgtg
     3481 actcgtgacc cgcgcggtga gcgcgcgcag ccgggtgcgc cggcgctgaa atcgctcggc
     3541 cgcggtggcg cccggatcgg tgcccaaatc cgtgacctcg agctcgaggc gttcgacgtg
     3601 cagcggcggg acgttcaagt ccgcgagcac gagccggccc acgccaccat gggcgtgaaa
     3661 cgtgaacggc agccgcttcg ttgcggctat tccctcgagc acggtcagaa gttaccagat
     3721 cgcgagtggt ttcatgtgcc tcgcgcccgt cgaagatcgt cgctggctag gccgttttgc
     3781 gaggcagctc gatcgtgaac gtcgtcgcct cgtgcgtcga cgcgactcgc gcctcaccgc
     3841 cgtgcgcttt cgcgatctgc tggacgatga agaggccgag gccgacactg cgcgatgcgg
     3901 tgcccttcga ggtcccgcgc gtcatgggga ggaaaatcga tgcgagctgg tcctcgggaa
     3961 tcgctggacc tgcattcgtg accgacagcg cgcatgccac gtcgtcgatg cgagatgcga
     4021 tctcgattgg cctcgtcgga ttgccgtagc tgacagcgtt cgaaacgaga ttcccgagca
     4081 gctggctgat ccgctgcgga tccgcgcgac acgttccgga gccctgggtc acgtgcgtga
     4141 gcgtgctctt tggatacgcg acgtgcagcg cgtcgactgc ctcggccacg gtcgaatgga
     4201 ggtcgacgtt cgtgacggaa atcgagatgc cattgccgac gcgtgcggcg gtgaagtcga
     4261 gcaggtcggc gagtagatgc gtcgcgcgat cgacggagcg atcgatcatc gcgacgagcc
     4321 ggtcctgcgc gttcggatcc ttgttggcga gcatctgcga cgccatcccg atcgtcgaca
     4381 ggggattgcg caggtcgtgg ctgacgatgc cgatcagctg ctccgcgaag tccgcccgat
     4441 cgcgctgttc cgtgacgagc gactcgagcc gctgcttcgc aagcacgagc tcttgctcgt
     4501 agcgatcgcg gtcgcgtccg atgaacacag caacgtcgag gacgatcgtc ccttcgtcat
     4561 cgcggcggac gacgttcacc accatcggga tcatggcgcc gtctcgatgg acgaagtcga
     4621 gcttcacttc ggacacggag ccctgcatcg cgagcagcgg cagccaatgc gtgtgatgaa
     4681 agatcctgct gcctaccccg agcaagtctt ggaagcgccg tttgccgacg agctcgtcgg
     4741 cggtgtagcc aagccacgcg cagaaggtgc cgttgacgcg acgaaccagg ccgtcgtcgg
     4801 cggtctgcat caagccgcag gccgcgtgct cgtacgcgtc gatcgtcacg aggctccgaa
     4861 acgctccttc gcgaggaacg catcgattgc ttccatcgtc gcgctcggcg cactcaggtg
     4921 cgggcagtgt ccgaggttct tgatgatcgt gagcgtgctg ttcggaatgt gcttgtgtag
     4981 gtagtcgccg acgctcttgg gcgcgatcag atcgtcgtcg cactgcacga gcagcgtggg
     5041 cgtcgtcagg cgagcgagat ccgcgcggtt gtcggagagg aacgtggcgc gcgcgaactg
     5101 cttcgcgatc tccggatccg tgcggcagaa gctgttcgtc agctcgacgc cgagctcggg
     5161 cttgtccggc gctcccatga tcgcgggggc catgctgctc gcccacccga ggtaattgcc
     5221 ttcgagtgtc gcgagcaggc cttcgatatc gccgcgcgag aacccgccga catagtcgcc
     5281 gtcgttgatg tagcagggcg aaggtccgat catgatctgc gcaccgaagc gctccggcgc
     5341 cgcgagattc gcgagcaacc cgatcatcgc gctcaccgaa tgaccgacga acagggcggg
     5401 gcctttgctc agctcgacaa cgaggtcgac cacgtcggcc gcgtaaccgt ccagcgacgc
     5461 gtacttcttg cggtcgtatg cactgaggtc cgagccgccg ctgccgacga ggtcgaacaa
     5521 cacaacgcgg tagctcttgg cgtagtgcgg cgcgatgaag cgccacatgt tctggtcgca
     5581 gccgaaccca tgcgcgaaga acatcgtcgc aggcccatcg cctttgacat gcacgttgag
     5641 cttcttgtgc aaatccatcc gtcccccacc ttacccagcc gctccgcaaa ggctacggcg
     5701 atcggttggc taaacgtttc gcaacgttca gcggaagctc atgcagactt tgtatgaggt
     5761 cgggagaacc ttcccggtgt cgtccgtctc gcgaatgagc gctggattga cgctgatgcg
     5821 gccatccttc gcgagtgtct cgcagcgctc cttcatccag ccttcgagga atgcttgcgc
     5881 gcgctgctgg cgcagctctt cgacgagacg atcagcttcc ttgtcgaact cttcgacctt
     5941 cggatcggcg cgcgtaatga gctgcatgac gtagtacgcg ccgtcacctt cgtagacgcg
     6001 cttcgcgagc atgcccggcg tgagctcgtc gaacagggcg gtgatcgctt ccttcgagct
     6061 gccgatgccg gacatcgtcg gcgtgcgagg cgcggggcca aagcgagcga cgtgcggcgg
     6121 ctcgacggtg ccgaactcgg ggagcttgtc ggtggacggc gtgagatccg caggcggtgg
     6181 aaccggtgcg ccggcggcgg gcgtcgcagt cggtgccgag gcagccgagc cagcgcccga
     6241 gcctgcagca gagccagccg cgggcggcgt cgccggcttc gttgcagcgg agcctgctgc
     6301 gggcggcgtt gcgggcttcg ttgcggcaga gccggccgag ccagttgcag cggcgggctt
     6361 cgcggaaccg gccgacgagc cgtcggcttg cgcgtaccac gcggccggga tgtccttgcc
     6421 ctcgacgaca tacgagcccg acttctgctg ttgcaggagc atctgcagga ccttctgctt
     6481 ttgctcgtcg ctcatgttcg ggtcgttgag gatctgctgg atgtcgatgc caggctgctc
     6541 cggcgctttc tctttctcgt acatctgatc gagattgagg ccgacgccgg catgcgcctt
     6601 gtcgagcgcg agcagcgctg cgcgcttcgc cgcttccttc gcccacacat cacgcgcgag
     6661 ctccttcgcg atctcgtgct tgacctggtc atagctgagg tcgccttcgc gcttctcttc
     6721 cgcgatcacg agccacgcgc cccggtccgt cgtgatcacc ggcgtcatct cgccggcctt
     6781 tagatccttg actgcatcgg acgcggcctt gtcgccgagc gatgcgttgt cggcggtgcg
     6841 ccaaccgagc tcgccgccat tagccttgaa gttctcgtcg gtgctgagct gcttcgctgc
     6901 gtcggcgaac ttctgcttgc cggcggcgat cgccgcgcgt gcggcctcga gcttggcctt
     6961 cgcctcttcg atcttcatgc cgaccgcctt ggcctccggc ttgggggccg gcggggcggc
     7021 tgcggggggc gctgcgctac cggctgcact gcctgctgcg ctaccggccg cactgccggc
     7081 ggcgctcccc tgatcggggg cgggcgtcgg ggcggcgggc tcctcgagct tcgcgatgaa
     7141 gatctggcgc agcttgagct gcggcttcac cgccttgtac gtgcgctcgt cggccttgaa
     7201 gcgcgcctcg acctcagcgc cgtgggtcga gatgaagcga tcgacgtcgg cgtcggtgag
     7261 cttcatcggc gtcttgtagt tctcggggcg gaacgcgacg acgtcataga ggaccgtgtt
     7321 gccctcgaac aaaaagtgct gcagcgcttc gtcgcgcgag acctgcacgc tatcgacgag
     7381 gatcttcgcc atgagtgccg cttggagcgc gcgctgttgc tccgagacgt acgccttgcg
     7441 cgtcacgttg agctggccga tccagttctt gaaggcctgg aggcgccaga ggccatcctc
     7501 gtcgaagatc tgcgggatcg tctcctcgcg acctgcatag aagaagtgac ccttcttgat
     7561 ctcttcctgg accaggtcct cgttgacgct gatgccgtgc ttctcggctt cccccgcgag
     7621 gatctcgcgg cggatcagca tctcgatcgc gccgtgcgtc ttgcgcttgc ccggtgccga
     7681 gctgagcgca tacgcgacgt gatacgcggt cggcgtgatg tctttaccgt cgacgcggac
     7741 gacggtgttg tccgaaccag tgcatccacc ttcgcgtccg ccaccctgcg ggccgaagtt
     7801 gatcacgaag atcgcgatca acagacagaa gatcaaatag acgaagatcg atgcaccctg
     7861 cttacgggcg ttttccagca tgggactgca gggttctaga acggggggct cttcggcaca
     7921 aggcgtctga agacccttcg ggtagctcaa gattgtcttg cgtgcggcgg tttttgcgtg
     7981 ataaccccgc caggcttttc ggattggtac gggccgaaag gcttcccttc ttctgcactt
     8041 ttccgctgcg cgtcacaggg cggcgttacc aggatcaaca tgctcttcga ttgggtctac
     8101 ggaatgtttt ccaacgacct ggccatcgac ctcgggacgg ccacgacgct cacgttcgtc
     8161 aaaggacgcg gcatcgttgc gaacgagccg agcgttgtcg ccgtacaggc cgtctcacaa
     8221 ggaccgaaga aggtcctcgc cgttggcaag gaagcgaaag agatgctcgg gcgaaccccg
     8281 ggcaacatcg tcgcgatccg gccgatgaag gacggcgtca tcgcagactt cgaagtgacc
     8341 gaagcgatgc tgcgctactt catccaaaag gcgcattcac gtcgcacgct cgtgcgtcca
     8401 cgcgtgatcg tgagcgtgcc gtcgggaatc accgaagtcg agaagcgcgc ggtgcgcgat
     8461 tcggcactcg cagcggctgc gcgcgaggtc tacctgatcg aggagccgat ggcggcggcg
     8521 atcggcggag gcctgccgat caccgagccc accgggaaca tgatcgtcga catcggcggc
     8581 ggtaccaccg aggtcgcggt gatcgcgctc gcgggcgtcg tgctctcgaa gtcgatccgc
     8641 gtcgccggcg acaagatgga cgaagcgatc gttcagtaca tcaagcgcaa gtacaacctc
     8701 ctcatcggcg agcgcaccgc cgagatcatc aagaaagaaa tcggcagcgc gtatccgctc
     8761 gaggagccga tgaccgtcga ggtgaagggc cgcgatctcg tcgccggcgt gccgaagacg
     8821 ctgctcgtca actccgacga gatccgcgaa gcgctcaccg agcccgtcaa cgcgatcgtc
     8881 gatgcggttc gctcggtgct cgagcgcacg cctcccgagc tgtcggccga catcgtcgac
     8941 aagggcatcg tgctctccgg cggtggcgcg cagctgaaga acatcgacgt gctgttgcgc
     9001 gaagagaccg gcctgcccgt gatggtctgc gagaacccgc agctcgccgt cgtgctcggc
     9061 accggcaaag tcctcgacga gctctcgctc ctccgagaaa tcgcgctgcg ctaggcgggc
     9121 gacccgccat gcagacgacg ctcaagcgca ggctgatcga ttggggcgcc acggcgttgc
     9181 tcgtgctgat ccccgtcctc gtcttgcgcg cgagccttca ccgcagcgag ccgtcgggtc
     9241 tcgacaaggc gctgcttcgc atcaccgcgc cgctgcaagc cggtgtctcg tgggtcgtcg
     9301 agggaatcgg cggcctgtgg ggacgctacg tcgcggtcgt cgatgtcgaa gacgagaacc
     9361 gcgagctgcg cgcagagaac gagcggctgc ggcgcgagct ctcgacgatg acgcgccggg
     9421 cgtacgacat ccaggcgctc gaggatctgg cggtcgtgaa gcgctcgacg ccagcggaca
     9481 cgctcggtgc gcgcgtgatc ggtgcgccga tgtctccaca gtttcgtgtg ctgcgcctgc
     9541 ggatcgatcg cggcgacaag gacgtgcaga tcggcatgcc cgtcatcacc ggtacgggcc
     9601 cagtcggtcg catcaacaaa gtctttggcg actacgcgga cgtcacgctc atcagcgatc
     9661 cgtcgtcgtc cgtcgaggtc gtcatcccgc gaacgggtgg acgcggcatc ctgcgcggcc
     9721 tcggtcgcaa cgattcgtac acgtgtgcga tcgagtggct cgagcgcagc aaggcgctgc
     9781 aaacgcccgc ggagtcgcgc gtgcagctcg gcgacgaggt cgtgacaagc ggtctcggcg
     9841 cgagcttccc gcccggcctc gtgatcggca aggtcagctc ggtcggcaag gacgacggca
     9901 tgttccagaa ggtagaggtc gatcccgccg tcgacgtttc gcgcgtgcgt gcagtcgaag
     9961 tgctgctcgc gccgccgccg ccgcccgacc ccgacgcgaa gaccaagcgc aagagcgagc
    10021 cggcattcgg cgggaggcct ctgtgatcac gctcctcatc atggcgatgg tcctgctcgc
    10081 gatgctcgcg gtggaagcgc cgctctggat ggtcgtcgcg atcctgattg cgtgggttct
    10141 cgtcgctggc ttcggaagga agaagcggtg agaacagcga cgatcgtcct cgtcgcatac
    10201 gtcgtgtgcg tgatcgttgc ggcgatctgg cgctacatgc cggggttcac gcgcgatgcg
    10261 gtgccggcga tcggtgcgct cacggctgca tatctcggcc acacgacgcg gccgtatcgc
    10321 tcgtcgtcga tcgcgggcgg cgtcgtgctc gggtacctga tcgacgtcat ctcgggcacg
    10381 cctccggggc tgtcctcgat cgcgctcgcg atgacggcga tggtcgctcg cggcacgcag
    10441 cagcggatct tcgtgcgtgg tgcagcgatg acgatcgcgt actccgcatt catcgcgctg
    10501 ttcgcatcgc tcgcgcgcct cgtgacgatg gtcttgttcg gcgtcccgcg cgcaccgagc
    10561 ttcgcgctcg agctgaagtt catggcgctg accgcgttcg cgacggcgct cgtcggtccg
    10621 atcgtgtggc ggctgttccg ccggatcgac gccgcgtacg cgcggacgca tcgcgagcgt
    10681 gacgcagcgc tcgagggtct cgcgccatga tgtatcggca ccagatccaa tcgagcaacg
    10741 gcggcgtcgg gctgcccgag ctgcacaagc gagtgaagtg gctcgcgatc atcgtcatcg
    10801 cagcgttcgc cctgctgatc ggccggctgt ggcaactcca agtcattcgc ggcgacagct
    10861 actacgagcg gacggtcagc aacgtcgtga aggagcggta cctgccgagc gttcgcggca
    10921 agatcctcga cagcaagggc atcccgctcg ccgacaaccg gccagcgttc aacatctacg
    10981 cgacgcccaa gcagctcacg cccgacgcta aggcagagct cgttcgcatg ctcggcctct
    11041 cggacgacga gatcggcaag ctcgacgaac gcatcgcggt aggcgtcaag cgcgacgc
//
LOCUS       DATLBZ010000086        10036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5290912, whole genome shotgun sequence.
ACCESSION   DATLBZ010000086 DATLBZ010000000
VERSION     DATLBZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5290912"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VMZ53_04600"
     CDS             <1..423
                     /locus_tag="VMZ53_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS27758.1"
                     /translation="VRSLPMPAVAVVSDQAHVAPALAAGARGVVLRDQVGPGIHAALA
                     AVRSGLTVMDAQLAESLVPAAPRATTNGKGRGELTEREKQVVQLLSEGLSNKLIADRL
                     GISDHTAKFHVNGVMMKLGSSTRTEAVVEAMRRGLIRL"
     gene            792..1463
                     /gene="moaD"
                     /locus_tag="VMZ53_04605"
     CDS             792..1463
                     /gene="moaD"
                     /locus_tag="VMZ53_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HUS27759.1"
                     /translation="MRIHVLYFAVFRERLRRDEEDLEIADGATVGHAIDALAARHADI
                     GKLRGRFRVAVNQDFVDDAHTLRDGDELALIPPVAGGSEGRARHVVLSDQPLSLDRCV
                     AAVAGPGMGGLVTFTGMVRLHSRGTTIDHLEYEAYAPMAVKVMTQLCEEIEAEVAGVR
                     LAVEHRVGNLAIGDIAVVIAAAAPHRAEAFTACRAMIDRLKDRVPIWKKEIGTGGEEW
                     VGLGP"
     gene            complement(1460..1726)
                     /locus_tag="VMZ53_04610"
     CDS             complement(1460..1726)
                     /locus_tag="VMZ53_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27760.1"
                     /translation="MQLASMVDVVARCIAKKDAPRTRLVAKSIDGVIAAFPTVGSSLV
                     IYRRGRSHRLVTSSVVRVLRDVDGATVYVETRNSVYRVTLRAAL"
     gene            complement(1800..3161)
                     /gene="gspN"
                     /locus_tag="VMZ53_04615"
     CDS             complement(1800..3161)
                     /gene="gspN"
                     /locus_tag="VMZ53_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HUS27761.1"
                     /translation="MAKLPFNPPQLGPRTRKILKYTAYVLFGIFTFVIALQLSFPYSR
                     VKDKLIDAASDKYTITVLKVERGLVPGRAYFKGVTITPVKTKPDEVVTPFYISELQVD
                     MGIMPLIGRKIVLDLDATIGDKKEGFGHLTVHVAVGKFGKGDIDAEIHGNALPSGALP
                     MRGLIGLPMSGKLNFLVDVSLPMEKSKMGKTSINWQRVTGEIALACPKDCVFGDGKTK
                     LKPLLKNTRNQVMVGEGIDFGKINIGSLNAHVSIKRGLLKLDKFDAKSGDGELKVDYE
                     MKLEKSFDESQVTGCLRFKGSDDLLRREPKTHAALSTTGAELRGDGLFHIRLTDRFKD
                     MKRLNQECGPNGQVGPTGGDSGGGGRDVRPNLTVQPDEAAKPVGSASITAPPAIPDVP
                     PATPLGQDAGAGSANPTGAAPPPTPAPGAHEQPPGTNFEPSTPNANHAGSGTGSAPTP
                     PPE"
     gene            complement(3164..3757)
                     /gene="gspM"
                     /locus_tag="VMZ53_04620"
     CDS             complement(3164..3757)
                     /gene="gspM"
                     /locus_tag="VMZ53_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HUS27762.1"
                     /translation="MSAVSDKARDFWDRISPRERRLVLIAAIAAPITIAIWLGLSIRD
                     GLVDMETRNEKTRKALRVLDGMRADGLPQQKGDDVVAMMGTEPLSLPTYLDTAAKAAG
                     FTLKTTSPHPSQTRNGFVTNSVSVTLEDLSIDQLKTFLSTVETNSKVVMVTHIDLRRD
                     FRDKAKLDVNLEVSTYSKEPPPKGDGAGSGSAAKKGS"
     gene            complement(3754..5412)
                     /gene="pilM"
                     /locus_tag="VMZ53_04625"
     CDS             complement(3754..5412)
                     /gene="pilM"
                     /locus_tag="VMZ53_04625"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HUS27763.1"
                     /translation="MATRMFAVDLGAWSVKLAIASPGIRGAALLDVREKMVPAGDEPT
                     EQRMLAGLKALIDELHLREDPGYIGVYGDQVFTQILEFGFKNLRRAELDKAVGGELEG
                     ELPVELDDMVYTFEQMPALPATGPVPAETHGRVALPADGMRVLTYAMRKDRAEHIIGI
                     GQQSGFQPRGLLACGGAATKLVAHVPSLMKARTDGAVAVIDIGHERTDVVVVYQNKAV
                     FSRSIPRAGKQVTEAIARNWKLPFDRAEQAKHADGFVASNQEPPQSDAWAKISNVLIQ
                     ELAPFARDLRQTIAACRAKTGFTPLGAILVGGGARLRGIGSFLSEQLAIPAWRLTRDD
                     AVALAGPRIDPNVATQLPIDTGAMTVGMAYDAAGGRPQFDLQSGALAAKMDLSFLRAK
                     AVQLGVAALAMAAFAAFSAYADLYRLRKSEKALTTRLANESTEVFGAPRTASEVLSSS
                     GANGSSQASPLPKMSAWDVMLEVSAHVPPKDKITLDIDKLDIDENKVDITGIAKTPKE
                     IDDFVVELKKIECFKEPQRGQTDTTEQGTKFRITIPSTCMKVNP"
     gene            complement(5415..6728)
                     /locus_tag="VMZ53_04630"
     CDS             complement(5415..6728)
                     /locus_tag="VMZ53_04630"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27764.1"
                     /translation="MSEDNQDKKPVVRKQQRGIALITVLIAIAITLVISNEFGTSTNV
                     DIGAAANYRDQMRAHFLARSAQNLAELVVRIQQRLDNIKELRGQVQITDYADQLILPF
                     CGDAEQVHDMIGMSTDQLKGMGAEIGTCGFDGPFTTEDNKINLNCANAPGATPAVIKS
                     VLDALVFFQAYDPVFEEADAEGWRRDRAQQVDAIMDYVDSNSMRGSNRGTAEDYGYES
                     LKDHYMPKNNYMDTIGEVKLVRGVDDRFWSLFGTAFTVYGGCKINLAAVTNVQLIASV
                     LYLSAKNPNDPVLQDQRKLFLLASIVAKAKMMGETFTKADDFVQFVKDPTASVSALAS
                     SGGMAGSAANDALTAGLGLAPGEKIGMELDPKNLNAIASFGPRRTYRIEAYGEVERKQ
                     KRADGQPVFPAIRSTINGVWDTKVFPQNVRKQPVPKGAWVYLRED"
     gene            complement(6725..7402)
                     /locus_tag="VMZ53_04635"
     CDS             complement(6725..7402)
                     /locus_tag="VMZ53_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HUS27765.1"
                     /translation="MRRSQAGMTLLEVMIASAIMVVMMTLAWKTISNTNDARRSTGVY
                     AERNHELRMALGRISADFEHAYLSKNEDINATHPRTLLVGKSGSKVPDIHVSTLGHRV
                     LWADANESEQTIVSYMAHNSKETPGQTDLIRSERRRPSNLNPDEEPAEYDVLVRDIDK
                     LELQYWNWKNLEWQDTWDTTQSDGQRGFLPTRVKITITVKAVDGRDIKLTTQARISMQ
                     EALIATP"
     gene            complement(7399..8184)
                     /locus_tag="VMZ53_04640"
     CDS             complement(7399..8184)
                     /locus_tag="VMZ53_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUS27766.1"
                     /translation="MSPRRTAGFTLLEVMLALALMGLALVVLIKSAAGNIASTEEAHM
                     MGIASDLARGKMYDIEEVLLKDGFTDTDQSQLGDQCFEDEGWPTVCYSYKVEEPKMPS
                     FDELQAMAQGQAQAMGSGMGSGFGSSFGSGFGSDMAQGGLENSALGGMLGMMGMFGGG
                     KQDIDAAQGGALIQSQYAMFQEILKVSVRKVTLTVKWKVLGKDRDMKVVAFFTDAAAM
                     DKVLSGMGSQELPDNSSTGTGTGSGGTKTPTPPRPGRGGSKTP"
     gene            complement(8181..8978)
                     /locus_tag="VMZ53_04645"
     CDS             complement(8181..8978)
                     /locus_tag="VMZ53_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HUS27767.1"
                     /translation="MKRPRAHRSQSGMTILEIMVVLAIMVVAIYMLRTGFRMVTKADL
                     VENSTELSAIMRRTQQLSVETAALHRVTFDMDKGLYVVEQCSGTSTIQQNEALRPDDE
                     KVKRAEEKGKDRLRNVPQQALTSDPEQATKQALALAGHHIADRVCVPVEDGLTGDVEK
                     DTHSERAWIRTLRAGKGIKFKEIWVQHREDSATKGQVAVYFFPNGSAEKAIIELTDGS
                     DTFSVLVFGLTGRVVLKDGELRDVEDHMLKNPLGDKDKERDAEGATE"
     gene            complement(8982..9392)
                     /locus_tag="VMZ53_04650"
     CDS             complement(8982..9392)
                     /locus_tag="VMZ53_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUS27768.1"
                     /translation="MKPLKHVARNSQRGMTLLEIMIVLAILALVMGLIVGPRVMKMFG
                     ESKVDIAKLTVQKFANEAFPQWSRSHPDKGCPDKIEELSEYMDKKDTKDPWGSEYKLL
                     CPPNLPPGVRTGIAIISAGEDKKEGTGDDIKSWE"
     gene            complement(9413..>10036)
                     /locus_tag="VMZ53_04655"
     CDS             complement(9413..>10036)
                     /locus_tag="VMZ53_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUS27769.1"
                     /translation="TSQGKTLPLNTRFLIWTSQFLGNWWFFILLGMILTGVLFSKWKA
                     SKEGKPIWHRFVLRLPVLGELVRTINVGRFARTLGTMLQSGVPMLRALDTAKQIVGNV
                     LIKQAIEDAKKAVTEGESLANTLRKSGQFQPTMIHMVSVGEKAGQLEQMLERVAMTFE
                     SEVDTKLSRFTALLEPLMLVVMGGAVAFIVFSILQPIMDLGQISGPK"
BASE COUNT         1600 a         3251 c         3219 g         1966 t
ORIGIN      
        1 gttcgctcgc ttccaatgcc cgccgtcgcg gtcgtttcgg atcaagcgca tgtcgcccct
       61 gccctcgcgg ccggtgcacg tggtgtcgtg cttcgcgacc aggtcggtcc cgggatccac
      121 gcggcgcttg ctgccgttcg cagcggcctg accgtgatgg atgcgcagct cgccgagtcg
      181 ctcgttccgg cggcgccgcg tgcgaccacg aacggcaaag gtcgtggcga gctgacggaa
      241 cgcgagaagc aagtcgtgca gctgctctcc gagggcctgt cgaacaagct catcgccgac
      301 cgcctcggca tcagcgatca caccgcgaag ttccacgtca acggcgtgat gatgaagctc
      361 ggctcttcga cccggaccga agcggtcgtc gaagcgatgc gccgcggcct gatccgactc
      421 tagacagtga ccccgctggt ggcagccagc cgtatgcatc gagggcgacg aggcccgttt
      481 cccccacgtt gatgccctcg tcctcgagca actgccgttg gacggctgcg ccgactggat
      541 cgtgaatcgc gatccggccg cgcagtcgtc cagcttttcc gatcacgcgt tgccacggca
      601 gccggtccgc gggcttgctc accttgagcg cagcacccgc aacgcgcgca ccgcccggaa
      661 ggcccgcgag ctccgcgacc tgcccgtacg tcgcgacgcg gccgcgcgga atcttgcgga
      721 ccaccttgta gatgcgtccg tacagctcgc gtacgtgatc cgtctcgttc acggtctgcg
      781 atactaccgc cgtgcgtatc cacgttctct acttcgccgt gtttcgcgaa cggcttcgtc
      841 gcgacgaaga agatctggag atcgcagatg gcgcaacggt cggccacgcg atcgatgcac
      901 ttgcagcgcg gcacgccgac atcggcaagc tgcgcgggcg atttcgcgtc gccgtcaatc
      961 aggactttgt cgacgacgca cacacgctcc gcgatggcga cgagctcgcg ttgatcccgc
     1021 cggtagctgg cggctcggag ggacgcgccc ggcacgtcgt gctgagcgat cagccgctct
     1081 cactcgatcg ctgcgtcgct gcggtcgcgg gtccgggcat gggcgggctc gtcacgttca
     1141 ccggcatggt gcggcttcac agccgaggca cgacgatcga tcacctcgag tacgaggcgt
     1201 atgcaccgat ggccgtgaag gtgatgacgc agctgtgcga agagatcgaa gccgaggtcg
     1261 ctggcgtgcg gctcgcggtc gagcatcgcg ttggcaatct cgcgatcggc gacatcgcgg
     1321 tcgtcatcgc ggcagcggca ccgcaccgcg ccgaggcgtt caccgcgtgt cgcgcgatga
     1381 tcgaccgctt gaaagaccgc gtgccgatct ggaagaaaga gatcgggacc ggcggcgaag
     1441 agtgggtcgg cctcggacct tagagcgccg cgcgaagcgt tacgcgataa acgctattgc
     1501 gcgtctcgac gtacacggtc gcgccatcga catcgcgcag cacgcgcacg accgagctcg
     1561 tcacgagccg atgactgcgg cctcggcgat agatcacgag tgagctgccg acggtcggaa
     1621 atgcagcgat gacaccgtcg atcgacttcg cgacgaggcg cgtgcgcggt gcgtctttct
     1681 tggcgatgca ccgagcgacc acatccacca tgcttgcgag ctgcacccac tgatcgtgag
     1741 gcctgcgggg tacgcgagac agtgatcgac cgggtactcg tcggtcagcc gtgcggtagc
     1801 tactcgggtg gaggtgtagg cgcagagccg gtgccggagc ctgcgtgatt ggcgtttggc
     1861 gtgctcggct cgaagttcgt acccggcggc tgctcgtggg cgcctggtgc cggcgtcggt
     1921 ggaggtgcgg cgcccgtcgg attggccgac ccagcgccag cgtcttggcc gaggggcgtc
     1981 gccggaggca cgtccgggat cgccggcggc gcggtgatcg acgcgctgcc cacgggtttg
     2041 gcagcttcgt cgggctggac ggtcagattc ggacgtacgt cgcgtccacc gccgcccgag
     2101 tcgccgccgg tcggacccac ttggccgttt ggaccacact cctgattgag gcgcttcatg
     2161 tctttgaagc gatcggtcag gcgaatgtgg aacaggccgt cgccgcgaag ctcggcgccc
     2221 gtcgtcgaga gtgcagcgtg cgtcttgggc tcgcggcgga gcagatcgtc gctgcctttg
     2281 aagcggaggc aacccgtgac ctgcgattcg tcgaagctct tctcgagctt catctcgtag
     2341 tcgaccttga gctcgccgtc gcccgacttc gcgtcgaact tgtcgagctt gagcaggccg
     2401 cgcttgatcg agacgtgcgc gttcagcgag ccgatgttga ttttgccgaa gtcgatgccc
     2461 tcgccgacca tcacttggtt gcgcgtgttc ttgagcaggg gcttgagctt ggtcttgccg
     2521 tcgccgaaca cgcagtcttt cgggcacgcg agcgcgattt cgccagtcac gcgctgccag
     2581 ttgatggacg tcttgcccat cttgctcttc tccatcggca acgacacgtc gacgaggaag
     2641 ttgagcttgc cggacatcgg caggccgatg agacctcgca tcggaagtgc accgctcggc
     2701 agcgcgttgc catggatctc ggcgtcgatg tcgcctttgc cgaatttgcc gacggcaaca
     2761 tggacggtga gatggccaaa gccttccttc ttgtcaccaa tggtcgcgtc gagatcgagg
     2821 acgatcttgc gaccgatgag cggcatgatg cccatgtcga cttggagctc ggagatatag
     2881 aacggcgtga cgacctcgtc gggcttggtc ttcaccggcg tgatggtgac gcccttgaag
     2941 tacgcgcgcc ccggcacgag cccgcgctcc actttgagca cggtgatcgt gtacttgtcg
     3001 gaagcggcgt cgatcagctt gtccttcaca cgcgagtacg ggaacgacag ctgcagcgcg
     3061 atcacgaacg tgaagatccc gaacaggacg tacgccgtgt acttgaggat cttgcgcgtg
     3121 cgcggtccga gctgcggcgg attgaacgga agcttcgcca tcgctagctc cccttctttg
     3181 cggcagagcc agagccggcg ccatcgccct tcggcggtgg ttccttcgag tacgtcgaca
     3241 cctcgaggtt gacgtcgagc ttcgccttgt cgcggaagtc gcggcgcaga tcgatgtgcg
     3301 tgaccatgac cactttgctg ttcgtctcga cggtcgagag gaacgtcttc agctgatcga
     3361 tcgacagatc ctcgagcgtg acggataccg agttcgtcac aaagccgttg cgcgtctgcg
     3421 acggatgcgg cgacgtggtc ttcagcgtga agcccgcggc tttcgcagcg gtgtcgaggt
     3481 acgtgggcaa gctgagcggc tcggtgccca tcatcgcgac gacgtcgtcg cccttctgct
     3541 gcggcagacc gtcggcacgc atgccgtcga gaacgcgaag cgccttgcgc gtcttctcgt
     3601 tgcgcgtctc catatcgacg agaccatcgc gaatcgagag gccgagccag atcgcgatcg
     3661 tgatcggcgc agcgatcgcg gcgatcagca cgaggcggcg ctcgcgcggc gagatgcggt
     3721 cccaaaaatc ccgtgccttg tcgctgaccg cgctcatggg ttcaccttca tgcaggtcga
     3781 cggaatcgtg atgcggaact tcgtgccctg ctccgtcgta tcggtctggc cgcgctgcgg
     3841 ctctttgaag cactcgatct tcttgagctc gaccacgaag tcgtcgatct ctttcggcgt
     3901 cttcgcgatg cccgtgatgt cgaccttgtt ctcgtcgatg tcgagcttgt cgatatcgag
     3961 cgtgatcttg tccttgggcg gcacgtgcgc actgacctcg agcatcacgt cccacgccga
     4021 catcttcggc agcggcgagg cttggctcga gccgttcgct cccgaggagc tcaggacctc
     4081 gcttgcggtg cgcggcgcac caaacacttc ggtcgactcg ttcgcgagac gcgtggtgag
     4141 cgccttttcc gatttgcgca ggcgatagag atcggcgtac gcgctgaacg cggcgaatgc
     4201 ggccatcgcg agcgcggcga cgcccaactg cacggccttc gctcgcaaga acgacagatc
     4261 catcttcgcg gcgagcgcgc cggattggag gtcgaactgc ggccggccgc ccgcggcgtc
     4321 gtaggccatg cccactgtca tcgctccggt atcgatcggc agctgcgtgg caacgttcgg
     4381 atcgatgcga gggcccgcga gagcgaccgc gtcgtcgcgt gtgagtcgcc atgcagggat
     4441 cgcgagctgc tcgctcagga agctgccgat accgcgcagg cgcgcaccac caccgacgag
     4501 gatcgcgccg agcggcgtga agccggtctt cgcgcgacac gctgcgatcg tctgccgcag
     4561 gtcacgcgcg aatggcgcga gctcctggat gaggacgttc gagatcttcg cccacgcgtc
     4621 ggattgcggc ggctcctgat tcgacgcgac gaagccatcg gcgtgcttcg cttgctcggc
     4681 gcgatcgaac ggcagcttcc agttgcgcgc gattgcctcg gtgacttgct tgccagcgcg
     4741 agggatcgag cggctgaaca ccgccttgtt ctgatagacg acgacgacgt cggtgcgctc
     4801 gtggccgata tcgatcaccg cgaccgcgcc atcggtgcgc gccttcatca gcgacggcac
     4861 atgcgcgacg agcttcgtcg cagcaccacc acacgcgagc aggccgcgcg gctggaagcc
     4921 ggactgttgg ccgatgccga tgatgtgctc cgcgcgatct ttccgcatcg cgtacgtgag
     4981 cacgcgcatg ccgtccgccg gcagcgcgac gcggccatgc gtctctgcgg gcaccgggcc
     5041 agtcgccggc agcgcgggca tctgctcgaa cgtgtagacc atgtcgtcga gctcgacggg
     5101 cagctcacct tcgagctcac cgccgaccgc cttgtcgagc tccgcgcggc gcaggttctt
     5161 gaagccgaac tcgaggatct gcgtgaacac ctggtcgccg tacacaccga tgtagccggg
     5221 gtcctcgcgc aggtgcagct cgtcgatcag cgccttgagc ccggcgagca tccgctgctc
     5281 cgtcggttcg tcaccggcgg gcaccatctt ctcgcgcacg tcgagcagcg ccgcgccgcg
     5341 aatgccaggg ctggcaatcg cgagcttcac gctccacgca cctagatcga ccgcaaacat
     5401 ccgagtagcc atgatcagtc ttccctcagg tacacccacg cgcctttggg cacgggctgc
     5461 ttcctgacgt tttgcggaaa cactttcgtg tcccagacac cattgatggt cgagcgaatc
     5521 gccgggaaca ccggctgccc gtccgcgcgc ttctgcttgc gctcgacctc gccgtacgcc
     5581 tcgatgcgat acgtgcgacg aggaccaaac gacgcgatcg cgttcagatt ctttggatcg
     5641 agctccatgc cgatcttctc gcctggcgcg agaccaagac cggcggtgag tgcgtcgttc
     5701 gccgcgctgc ccgccatgcc gccgctgctc gcgagtgccg acaccgacgc cgtcggatcc
     5761 ttcacgaact ggacgaagtc gtcggccttc gtgaacgttt cgcccatcat cttcgccttc
     5821 gcgacgatgc tcgcgagcag gaacagcttg cgctggtctt ggaggaccgg atcgttcgga
     5881 ttcttcgccg agagatagag caccgacgcg atcagctgca cgttcgtgac cgcagcgagg
     5941 ttgatcttgc agccgccgta gacggtgaag gccgtgccga acagcgacca gaaccgatcg
     6001 tcgacgccgc gcacgagctt cacctcgccg atcgtgtcca tgtagttgtt cttcggcatg
     6061 tagtgatctt tgaggctctc gtagccgtag tcctcggcgg tgccgcggtt ggagccgcgc
     6121 atcgagttcg aatcgacgta gtccatgatt gcgtcgacct gctgcgcgcg atcacggcgc
     6181 caaccttcgg catcggcttc ctcgaacacc gggtcgtacg cttggaagaa cacgagcgcg
     6241 tcgagcacgc tcttgatcac tgcgggcgtc gcgcccggag cgttcgcgca gttgagattg
     6301 atcttgttgt cctcggtcgt gaacggaccg tcgaagccac aggttccgat ctcggcgccc
     6361 atgcccttca gctgatccgt cgacatgccg atcatgtcgt gcacctgctc ggcgtcgccg
     6421 cagaacggta ggatcagctg atcggcgtag tccgtgatct ggacctgacc gcggagctcc
     6481 ttgatgttgt cgaggcgctg ctggattcgg acgacgagct cggcgaggtt ctgcgcagac
     6541 cgcgcgagga agtgcgcgcg catctgatcg cggtagttgg cggccgcacc gatgtcgacg
     6601 ttcgtcgacg tgccgaactc gttcgagatg acgagcgtga tcgcgatcgc gatgagcacg
     6661 gtaatgagcg cgatgccgcg ctgctgcttg cgcacgaccg gcttcttgtc ttggttgtct
     6721 tcgctcatgg cgttgcgatc agcgcttcct gcatcgagat gcgggcctgc gtcgtgagct
     6781 tgatgtcgcg gccatccacg gccttgacgg tgatcgtgat cttcacgcgc gtcggcagaa
     6841 agcctcgctg gccgtccgac tgcgtcgtgt cccacgtgtc ctgccactcg aggttcttcc
     6901 agttccagta ctggagctcg agcttgtcga tgtcgcgcac gagcacgtcg tactcggctg
     6961 gttcttcgtc tggattgagg ttcgacggcc ggcgtcgctc gctgcgaatg agatcggttt
     7021 gccccggcgt ctccttcgag ttgtgcgcca tgtacgagac gatcgtctgc tcgctctcgt
     7081 tcgcgtccgc ccacagcacg cggtggccca gcgtcgacac gtggatgtcg gggaccttcg
     7141 agccggactt gccgacgagc agcgtgcgtg gatgcgtcgc gttgatgtcc tcgttcttcg
     7201 acagatatgc gtgctcgaag tcggcgctga tgcgcccgag cgccatgcga agctcgtgat
     7261 tgcgctccgc gtacacgccc gtcgagcgcc gcgcgtcgtt cgtgttcgag atcgtcttcc
     7321 atgcgagcgt catcatcacg accatgatcg cgctcgcgat catgacctcg agcaacgtca
     7381 tgccggcctg cgagcgcctc atggcgtctt gctcccaccg cgacccgggc gcggcggcgt
     7441 tggcgtcttc gtgccaccgg atcccgtacc cgtgcccgtg ctgctgttgt ccggaagctc
     7501 ttgcgagccc atgccggaga gcactttgtc catcgcggcc gcgtcggtga agaacgcgac
     7561 gaccttcatg tcgcgatcct tgccgagcac tttccacttc acggtcagcg tgaccttgcg
     7621 aacggagacc ttcaggatct cctggaacat cgcgtactgg ctctggatga gcgcgccgcc
     7681 ctgcgctgcg tcgatgtcct gcttgccacc gccgaacatg cccatcatgc cgagcatgcc
     7741 gccgagcgcg ctgttctcga gaccgccctg cgccatgtcc gagccgaagc ccgagccaaa
     7801 gctcgagcca aaaccagagc ccatgcccga tcccatggcc tgcgcctggc cctgcgccat
     7861 cgcctgcagc tcgtcgaagc tcggcatctt cggctcttcg accttgtacg agtagcagac
     7921 ggtcggccag ccctcgtcct cgaagcactg gtcgccgagc tgcgactgat ccgtatccgt
     7981 gaagccgtct ttgaggagga cctcctcgat gtcgtacatc ttgccgcgcg cgagatcgct
     8041 cgcgatgccc atcatgtgcg cttcttccgt cgaagcgatg ttgccggccg cgctcttgat
     8101 gagcacgacg agcgcgaggc ccatcagcgc gagcgcgagc atcacctcga gcagcgtgaa
     8161 tcccgccgtg cgtctcgggc tcattcggtc gctccctcgg cgtcgcgctc tttgtccttg
     8221 tcgccgagcg ggttcttcag catgtgatct tcgacgtcgc gcagctcgcc gtctttgagc
     8281 acgacgcggc cggtaagacc gaacacgagc acgctgaacg tgtcgctgcc gtcggtgagc
     8341 tcgatgatcg ccttctcggc cgagccgtta ggaaagaagt agacggccac ctgccccttc
     8401 gtcgcgctgt cctcgcgatg ctggacccag atctctttga acttgatccc cttgcccgcg
     8461 cgcagcgtgc gaatccacgc gcgttcggag tgcgtgtcct tctcgacgtc gccggtcagg
     8521 ccgtcttcga cgggcacaca cacgcgatcg gcgatgtggt gaccggcgag cgcgagcgcc
     8581 tgcttcgtcg cttgctcggg gtcgctcgtc agcgcctgct gcggaacgtt gcgcaggcga
     8641 tccttgccct tctcttcggc gcgcttgacc ttctcgtcat cgggacgcaa cgcctcgttt
     8701 tgctggatcg tcgacgtgcc ggagcactgc tcgacgacat agaggccttt gtccatgtcg
     8761 aacgtgacgc gatgaagcgc cgcggtctcg acggagagct gttgcgtccg gcgcatgatc
     8821 gccgagagct cggtcgagtt ctcgacgagg tccgccttgg tgaccatgcg gaagcccgtg
     8881 cgcagcatgt agatcgccac gaccatgatg gcgaggacca ccatgatctc gaggatggtc
     8941 atccccgatt gcgatctatg tgcgcgcggg cgtttcatgg atcattccca ggacttgatg
     9001 tcgtcgccgg tgccctcttt cttgtcttcg ccggccgaga tgatcgcgat gccggtgcgg
     9061 acgccgggcg gaaggttcgg cgggcacagc agcttgtact cgctgcccca cggatccttc
     9121 gtgtccttct tgtccatgta ctcggacagc tcctcgatct tgtcgggaca gcccttgtcg
     9181 ggatgcgagc gcgaccactg cgggaacgcc tcgttcgcga acttctgaac ggtgagcttg
     9241 gcgatgtcga ccttcgactc gccgaacatc ttcatgacgc gaggaccgac gatgaggccc
     9301 atcacgagag ccaagatcgc gaggacgatc atgatctcga gcagggtcat accgcgctgg
     9361 ctgttacgag cgacgtgctt caggggcttc atacggttct ccatttgtct tctcacttcg
     9421 ggccggaaat ctgaccgagg tccatgatgg gttgcaggat cgaaaacacg atgaacgcga
     9481 cggcgccgcc catgacgacg agcatcaggg gctcgagcag cgcggtgaat cgcgacagct
     9541 tggtgtcgac ttcggactcg aacgtcatcg cgacgcgctc gagcatttgt tcgagctgac
     9601 cggctttctc gccgacggac accatgtgga tcatcgtcgg ctggaactgg ccgctcttgc
     9661 gcagcgtgtt cgcgagcgat tcaccctcgg tgaccgcctt ctttgcatct tcgatcgcct
     9721 gcttgatgag cacgttgccg acgatctgct ttgcagtatc gagcgcacgc agcatcggga
     9781 cgcccgactg cagcatcgtg ccgagcgtgc gagcgaagcg accgacgttg atcgtgcgca
     9841 cgagctcgcc gaggaccggc agcctgagca cgaagcggtg ccagatcggc ttgccctcct
     9901 tgctggcctt ccacttcgag aacaggacgc ctgtgaggat catgccgagg aggatgaaga
     9961 accaccagtt gccgaggaac tgcgaggtcc agatgaggaa ccgcgtgttc agcggcagcg
    10021 tcttgccctg cgacgt
//
LOCUS       DATLBZ010000087         4005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2309055, whole genome shotgun sequence.
ACCESSION   DATLBZ010000087 DATLBZ010000000
VERSION     DATLBZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309055"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VMZ53_04660"
     CDS             complement(<1..203)
                     /locus_tag="VMZ53_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS27770.1"
                     /translation="MVRALAAFVGLALVGSSPALAQNAEGARLFEEGRALAKDGKYVE
                     ACATFEKSLQLDPAPGTKLNYGD"
     gene            complement(242..2329)
                     /locus_tag="VMZ53_04665"
     CDS             complement(242..2329)
                     /locus_tag="VMZ53_04665"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27771.1"
                     /translation="MTRAPAEVGSAANSYEILAKLAMGGMAEIFLARGASSAGVERYV
                     VLKRVLRHRASDPHFVRMFLDEARLAAQLQHPNIAQVYDIGKLGDSFFFTMEYVHGET
                     VRTLLQRSHALRRTIPIGSVLSIIAGSAAGLHHAHERKSMDGRPLGIVHRDVSPSNLM
                     VSYEGTVKVVDFGVAKAEHRSTETQSGTVKGKITYMSPEQCKGGDMDRRSDLFSLGIV
                     MWEMLTLERLFRRNSDFENMQAIVAEDVQPPSRFRPDIPPEVDHIVMRLLAKSPSDRY
                     QNADELHEAIEAAAVRTGSALSAASLGRYMRELFGQRPEPWIELQSNDQHPEAFTVTS
                     EPIPAQLAVSPADDLDRKLQAIPQLSARMSAQMGAQPAPLAMPTVPLRVQADPDLAMT
                     IQQPPRNVGPVPVPQPMPQMQSSSAQVPGFIATTPGSGLPMRPHSPSVTPGSNPQYPM
                     LSSASHAAYVIGRQSQSSMQPLPQPPRRRLGLYSLVPAVILGVVFGLALGFGGEKKPV
                     ETAKTEPPAEEEIVIEKSMPVVPTPTPPTPTTIEAGSAANKKCDPFSDMHNCEPADAK
                     PEPETKPETKPEADTKPETETKTVPKTTTPKSKTEHKTDEPAVSDLYKDKKYADVFDA
                     CASSSKVVALNATNCTLAACKLKETSKAKKWFGQVGAAKKPAVQKECGAVLPADKASA
                     DEQCKRDPMACQR"
     gene            complement(2456..>4005)
                     /locus_tag="VMZ53_04670"
     CDS             complement(2456..>4005)
                     /locus_tag="VMZ53_04670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HUS27772.1"
                     /translation="GSRAPRPRDEEERLLAVGGVGMIGDAMSWVLSTLKRSLGARTVA
                     LLWVEDSDGDRVKLMEVASDADDITESPKLPSAGVLGAVLRDRQPLLVGATKPGQLPY
                     YDSGRAGVALVAVPILEGGHLRGILAADRDAMFTEADRDLLVDAGSQVLRVVQAEQVF
                     RAVERAKYEHERFYLATAMLGRALTPEQVMETAFDACAQIVDYDAAAIALYDKDRAKH
                     RIAAVRIGKDGEGIIDPKLPGFEFKDNAGLASMVIKNRHYLPAGGEPREVTAPIYTRK
                     IKIDEAKSLIILPLLSADEAIGTFTLVARAEKRFGNDVREMLAVIANQVAISLQNGYL
                     YKQMETMATTDGLTGLTNHRTFQARFEDLLQRAARHGHKVALLLCDVDHFKKVNDGYG
                     HPIGDEVLRRVAKVLQEVPRKIDIPARYGGEEFAVLLDNVDVAQAKAVAERIRIEISK
                     VVVETDKGPLSVTESVGVAAFPDDGRDRATLIERADLALYHAKHTGRNRVVTWTEAQA
                     AKAKQAS"
BASE COUNT          613 a         1340 c         1301 g          751 t
ORIGIN      
        1 cagtcgccgt agttcagctt cgtgccgggc gcgggatcga gctgcaggct cttctcgaac
       61 gtcgcgcacg cttcgacgta cttgccgtct ttcgcgagtg cgcggccctc ttcgaacagt
      121 cgtgcaccct cggcattctg cgcgagcgcc gggctcgaac cgacgagagc gagccccacg
      181 aacgcagcca acgcgcgcac catggctcta tcgtcacgca actcctcggc agccgcaaca
      241 gctagcgctg gcaggccatc gggtcgcgct tgcactgctc gtcggcgctc gccttgtcgg
      301 ccggcaggac ggcgccgcac tctttctgca cggcgggctt cttcgcagcg ccgacttggc
      361 caaaccactt cttcgccttc gacgtttctt tgagcttgca ggccgcgagc gtgcagttcg
      421 ttgcgttgag cgcgacgacc ttcgacgacg acgcgcacgc atcgaacacg tcggcgtact
      481 tcttgtcctt gtacagatcc gagacggcgg gctcgtcggt cttgtgctcg gtcttcgact
      541 ttggcgtcgt cgtcttcggg acggtcttcg tctcggtctc cggcttcgtg tcggcctcgg
      601 gcttcgtctc cggcttcgtc tcgggctccg gtttcgcgtc agccggctcg cagttgtgca
      661 tgtccgagaa cggatcgcac ttcttgttcg cggcggagcc cgcttcgatc gtcgtcggcg
      721 ttggtggcgt gggcgtcggc acgacgggca tcgacttctc gatgacgatt tcctcttcgg
      781 cgggcggctc cgttttcgcg gtctctaccg gcttcttctc gccgccaaag ccaagcgcga
      841 ggccgaacac gacgccgagg atcacggcgg gcacgagact gtagaggccg agccgtcggc
      901 gtggcggttg cggcagcggc tgcatgctcg actgcgactg ccgcccgatc acgtacgcag
      961 cgtgtgacgc cgacgacagc atcggatact gcgggttgct gcccggcgtg accgacggcg
     1021 agtgcggccg catcggcagt ccggagccag gcgtcgtcgc gatgaagccc ggcacctgcg
     1081 cactcgagct ctgcatctgc ggcatcggct gcggcacagg gaccggcccg acgttgcgag
     1141 gcggctgctg gatcgtcatc gccagatccg gatccgcttg cacgcgcagc ggcacggtgg
     1201 gcatcgcgag cggcgccggt tgcgcgccca tctgcgcaga catccgcgcc gacagctgcg
     1261 gaattgcctg gagcttgcga tcgagatcgt cggccggcga caccgcgagt tgcgcgggaa
     1321 tcggctcgct cgtgacggtg aatgcctcgg gatgctgatc gttgctctgc agctcgatcc
     1381 acggctcggg ccgctggccg aacagctcgc gcatgtaacg gccgaggctc gccgccgaga
     1441 gtgcggagcc cgtgcgtact gcagccgctt cgatcgcttc gtgcagctcg tcggcgttct
     1501 gatagcgatc gctcggcgac ttcgcgagca gccgcatgac gatgtgatcg acctcgggcg
     1561 ggatgtcggg ccggaacctc gacggcggct gcacgtcctc ggcgacgatc gcctgcatgt
     1621 tctcgaagtc gctgttgcga cggaacaacc gctcgagcgt cagcatctcc cacatgacga
     1681 tgccgagcga gaacaggtcg ctgcgtcgat ccatatcgcc gcccttgcac tgctcgggcg
     1741 acatatacgt gatcttgccc ttcacggtgc ctgactgcgt ttcggtgctg cggtgctccg
     1801 ctttcgcgac gccgaagtcg acaaccttga cggtgccctc gtagctgacc attaggttcg
     1861 acggcgagac gtcgcgatgg acgatgccga gcggcctgcc gtccatgctc ttgcgctcgt
     1921 gcgcgtgatg caggcccgcg gcggagccgg caatgatcga gagcacgctg ccgatcggga
     1981 tcgttcggcg cagcgcatgc gatcgctgga gcagcgtgcg cacggtctcg ccatgcacgt
     2041 actccatcgt gaagaagaac gaatcgccga gctttccgat gtcgtagacc tgcgcgatgt
     2101 tgggatgctg cagctgcgca gccaaccgcg cttcgtcgag gaacatgcga acgaagtgcg
     2161 gatcgctcgc gcggtgacga agcacgcgct tgagcacgac atatcgctcg acgcccgcgc
     2221 tgctcgcacc acgcgcaagg aagatctcag ccatgcctcc catcgcgagc ttcgcgagga
     2281 tttcatagct gttcgcggca gagccgacct ctgcaggcgc tcgggtcacc gtctctaatt
     2341 gtacgccacg atcacgagcg ctcgcgccag cgcacgacca cgcacacgaa cacctacata
     2401 cgtattctcg cgattgcgca cgatcgtgcg cagatctgcg aaccgatcca gcgcgctagc
     2461 tcgcctgctt cgccttcgca gcttgcgctt cggtccacgt gaccacgcga ttacgacccg
     2521 tgtgcttcgc gtgatagagc gcgaggtccg cgcgttcgat gagcgtcgcg cgatcgcggc
     2581 cgtcgtcggg gaacgcggca acgccgacgg actcggtgac ggacagcggg cccttgtcgg
     2641 tctcgaccac cactttggag atctcgatgc gaatgcgctc ggcgaccgcc ttcgcttgcg
     2701 cgacgtcgac gttgtcgagc agcacggcga actcctcacc gccgtagcgc gcagggatat
     2761 cgatcttgcg cggcacttcc tgcagcacct tggcgacgcg acgcaggacc tcatcgccga
     2821 tcggatgacc gtagccgtcg ttgaccttct tgaagtggtc gacgtcgcac aggagcagcg
     2881 cgactttgtg gccgtggcgt gcggcgcgct gcagtagatc ttcgaagcga gcttggaacg
     2941 tgcggtgatt cgtcaggccg gtgaggccgt ccgtcgtcgc catcgtctcc atctgcttgt
     3001 acaggtatcc gttctgcagc gagatggcga cttggttcgc gatcaccgcg agcatctcgc
     3061 gcacgtcgtt gccgaagcgc ttctcggcgc gcgcgacgag cgtgaacgtg ccgatcgctt
     3121 cgtcggccga cagcagcggc aagatgatga gcgacttcgc ctcgtcgatc ttgatcttgc
     3181 gcgtgtagat cggcgcggtg acttcgcgtg gctcgccgcc cgcaggcagg taatggcgat
     3241 tcttgatcac catcgaggcg aggcctgcat tgtccttgaa ctcgaagccg gggagcttcg
     3301 gatcgatgat gccctcgccg tccttgccga tgcgcacggc tgcgatgcgg tgcttcgcgc
     3361 gatctttgtc gtagagcgcg atggcagcgg cgtcgtagtc gacgatctgc gcgcacgcat
     3421 cgaacgcagt ctccatgacc tgctccggcg tcagcgcacg gccgagcatc gcggtcgcca
     3481 gatagaaccg ctcgtgctcg tacttcgcgc gctcgacggc gcggaacacc tgctccgcct
     3541 gcacgacgcg caacacctgg ctgcctgcgt cgacgagcag atcgcgatcc gcttcggtga
     3601 acatcgcatc gcgatccgcc gcgaggatgc cgcggaggtg cccgccctcg aggatcggca
     3661 cggcgacgag cgcgacgccc gcacggccgc tgtcgtaata aggcagctgg ccgggcttcg
     3721 tcgcaccgac gagcaacggc tggcgatcgc gcagcaccgc gcccaggacg cccgcgctcg
     3781 gcaacttcgg cgattctgta atgtcgtcgg catcgctcgc gacttccatg agctttacgc
     3841 gatcaccgtc ggagtcttcg acccagagca gcgcgaccgt gcgtgcaccg agcgagcgct
     3901 tgagcgtcga cagcacccag ctcatcgcgt cgccgatcat gccgacgccg ccgaccgcga
     3961 gcaggcgctc ttcttcatcg cgcgggcgtg gtgcacggct gcccg
//
LOCUS       DATLBZ010000088         5945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2309229, whole genome shotgun sequence.
ACCESSION   DATLBZ010000088 DATLBZ010000000
VERSION     DATLBZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309229"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..375)
                     /locus_tag="VMZ53_04675"
     CDS             complement(40..375)
                     /locus_tag="VMZ53_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27773.1"
                     /translation="MSRQSRIKRLYSRRLARKLAATTGAARTELEALAEYESRHEIAR
                     QRDKGWKLVTKLETGEGVFVRDDDVVGLHIQLPTALYKRLDTEANQRETTKRQMVIAA
                     LERYLDQTH"
     gene            complement(481..1362)
                     /locus_tag="VMZ53_04680"
     CDS             complement(481..1362)
                     /locus_tag="VMZ53_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27774.1"
                     /translation="MTRALAMSLLLLGACDNVFRIIRVDERVDGNTGDGITADGPVDA
                     AKQGCALNFISPGDFDTDGIANDVDLCPALTSPQSDYDGDGLGDMCDPHMMTPGDCIL
                     LMDTFDNLDCWHSYEPWNGCNAEPMRPCSTVAVSTLDLLTPLGMTSAELRGTVVNVQG
                     TMPNVGLMMDDSQLGAGITGHRCAIARDPNGYIESLSTLYSNGTPVSNMPQTNSPTQM
                     MDMVPFSAVIVWQPQQAVTPYVDACETYTASTTVPISMGRIYSTSNPAGPNAVIRSAD
                     MVFRPTAFIAYGACSDN"
     gene            complement(1373..2104)
                     /locus_tag="VMZ53_04685"
     CDS             complement(1373..2104)
                     /locus_tag="VMZ53_04685"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HUS27775.1"
                     /translation="MTFLESVGSAVRSQRERKGWSRRELASASGVSERFLAQLETGDG
                     NISLRRFAEVAHALGTTPASLLAPAEHPRTTKPIALLGVRGAGKSTIGAALARKLDMR
                     FVEVDQEIEEATGLPLGDVFTLHGEAYYRRVEREVLTRLLADHTPMVLATGGSIVNDP
                     TNFALLKSRAHTIWLRAKPEDHWNRVVAQGDQRPMAENPHAFEELKALLAARQKLYGR
                     ADLAIDTSERTIKQVVAAIAADMPQ"
     gene            2169..3791
                     /gene="boxC"
                     /locus_tag="VMZ53_04690"
     CDS             2169..3791
                     /gene="boxC"
                     /locus_tag="VMZ53_04690"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HUS27776.1"
                     /translation="MDPTSFETHPSRYKHWRLTTNGDIATLVMAVDGDHPHRPGYELK
                     LNSYDLSVDIELADAIQRLRFEHPEVKCVVITADLDRVFCSGANIYMLGQSTHSFKVN
                     FCKFTNETRLYLEDASAQSGLTSLAACKGTTAGGGYELALACDETMLVDDGSSAVSFP
                     ETPLLAVLPGTGGLTRLVDKRKVRRDRADVFCTVAEGIKGKRAKDWGLVDHLVSRTKW
                     DETVAQRAKELAAKQQVTRGPAFVLAPLEAKVTATKLTYRFVEFTVDAAARTGNLLIK
                     GPDAVDTFDGNAEPWSLRAFRELDDALLHLRFDYPEINVVTVRTSGKQDIVRDCDEAL
                     AKASSGFAREIRLLQRRVLKRYDNTARSLYAVADNTDSCFAGSLLELALGADRFYMLI
                     DGDEKIGVTTSVANSGFMTTSTGLSRLDARFYGDPSQVQKILARGAEGFISSEDAEKI
                     GIATVAADDIDFEDELRIAIEERASLSPDALTGMEASLRFVGPETLETKIFGRLSAWQ
                     NWIFTRPNSTGQHGALTLYGQPERPQFQWKRT"
     gene            3795..5207
                     /gene="boxB"
                     /locus_tag="VMZ53_04695"
     CDS             3795..5207
                     /gene="boxB"
                     /locus_tag="VMZ53_04695"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HUS27777.1"
                     /translation="MSAILNDDKIPNNVNLSSDKRLQRALEAWQPKFIDWWKDMGPVG
                     WQERDIYLRTATSVEANGWAHFDYVKMPDYRWGIFLEPKDPNRVHGFGDMEGQPVWDE
                     VPGEFRNMLRRLVVTQGDTEPASVEQQRNLGATAPSLYDLRNLFQVNVEEGRHLWAMV
                     YLLHTYFGRDGREEAEELLQRRSGDADKPRILGAFNEPTSHWLSFFMFTMFTDRDGKY
                     QLAALAESGFDPLARTCRFMLTEEAHHMFVGETGVQRVVKRACELMKQEPNGDVRKLG
                     GVDLPTIQKYLHLWYTLSLDLFGGEVSSNAADAFAAGLKGRYKEDRYPEHKLIGSTFA
                     MDVLEDGKVVHKDIAMRNALNEVLRTEYIKDCQRGVDRWNKTIKDEGIDFELKLPSNK
                     FHRQIGMWSNHRFSPSGDMLDQATWDAKHGEWLPNAADETYIKSLMKANYERGKMANW
                     IAPPLKGINGLPEDFEYVKL"
     gene            complement(5727..>5945)
                     /locus_tag="VMZ53_04700"
     CDS             complement(5727..>5945)
                     /locus_tag="VMZ53_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27778.1"
                     /translation="YLLPRDPTTKRCACMDAEGACTIYDQRPATCRTYDCREDARVWI
                     DYEKRIPAPLPASIRPAGYIDPNDKKSE"
BASE COUNT         1100 a         1934 c         1902 g         1009 t
ORIGIN      
        1 tcgccggtaa cgccgtagag aattctcatc gagagcctcc tagtgagttt gatcgaggta
       61 ccgctcgagc gcggcgatca ccatctgccg cttcgttgtc tcgcgctggt tggcctcggt
      121 atcgagtcgc ttgtagagcg ccgttgggag ctggatgtgg agaccgacaa catcgtcatc
      181 gcgcacgaac acgccttcgc cggtctcgag cttggtgacg agcttccagc ccttgtctcg
      241 ctggcgggcg atctcgtgcc gcgactcgta ctcggcgagg gcctcgagct cggtgcgggc
      301 ggcgccggtc gtggcggcga gcttgcgggc gagtcgacgt gagtacaacc gcttgatgcg
      361 actttggcgt gacatggcgt gtcttgttct ttgtacaatg tacaagacac ggaagcacag
      421 tcaagtggca acaaccggca agccgccggc tgcagcccga caagcgcgtt tggacggcgc
      481 ttagttatcc gagcaggcgc cgtacgcgat gaacgcggtc ggacggaaga ccatgtctgc
      541 ggagcgaatc acggcattcg ggccagcggg attggacgtc gagtagatgc ggcccatcga
      601 gatcgggacc gttgtggagg ctgtgtacgt ctcgcacgca tcgacatacg gcgtcaccgc
      661 ttgctgcggc tgccacacga tgaccgcgga gaacgggacc atatccatca tctgggtggg
      721 cgagttcgtc tgcggcatgt tcgaaacggg cgttccgttc gagtacagcg tcgataacga
      781 ctcgatatag ccgttcgggt ctcgcgcgat cgcgcagcga tgtccggtga tccccgcgcc
      841 tagttggctg tcgtccatca tgaggccgac gttcggcatc gtgccttgca cgttcacgac
      901 ggtcccgcgc agctcggccg acgtcatccc gagcggggtg agcaaatcga gcgtgctcac
      961 ggcgacggta gagcacgggc gcatcggctc ggcgttgcag ccgttccacg gctcgtagga
     1021 gtgccagcag tcgaggttgt cgaacgtatc catcagcagg atgcagtcgc ctggcgtcat
     1081 catgtgcgga tcgcacatgt cgccaagccc gtcgccgtcg tagtcgctct gcgggctcgt
     1141 gagagcgggg cacaggtcga cgtcgttggc gatgccgtcg gtatcgaagt cgcccggcga
     1201 gatgaagttc agcgcgcagc cttgcttcgc tgcgtcgacg gggccgtcgg ccgtgatgcc
     1261 atcgcccgtg ttgccatcga cgcgctcgtc gacgcgaatg atccgaaaca cgttgtcaca
     1321 cgcaccgagc aacagcagcg acatggcgag agcgcgtgtc acgggggagg actcattgtg
     1381 gcatgtcagc ggcaatcgct gcgacgacct gtttgatcgt gcgctcgctg gtgtcgatgg
     1441 ccaggtcggc gcgaccgtag agcttttggc gggcggcgag taaggccttc agctcctcga
     1501 acgcgtgtgg gttctcggcc atcgggcgtt ggtcaccctg cgcgacgacg cgattccagt
     1561 gatcctccgg cttcgcgcgc agccagatgg tgtgggcgcg gctcttgagc agcgcgaagt
     1621 tcgtcgggtc gttgacgatc gagccgccgg tcgcgagcac catcggcgtg tggtcggcga
     1681 gcagccgagt caggacctcg cgctcgacgc gccggtagta cgcctcgccg tggagcgtga
     1741 atacatcgcc gagcgggagg ccggtcgctt cctcgatctc ctgatcgact tcgacgaagc
     1801 gcatgtcgag cttgcgtgcg agcgcggcgc cgatcgtcga tttgccggca ccgcgaacgc
     1861 cgagcagcgc aatgggcttg gtcgtgcgcg gatgctccgc cggcgcgagg agcgatgccg
     1921 gcgtcgtacc gagcgcgtgc gcgacctcgg cgaaccggcg cagcgagatg ttgccgtcgc
     1981 ccgtctcgag ctgggcgagg aaccgctccg agacgccgct cgcggacgcg agctcccgtc
     2041 gagaccagcc cttgcgctcg cgctgcgaac ggacggccga tccgacggat tcgaggaagg
     2101 tcacgcctgc actatagtgc atgcagaacg gacgtgacaa gcagtaaatt gcttgataga
     2161 cgatcgtcat ggatccgacg agcttcgaga cccacccgag ccgctacaag cactggcgtc
     2221 tcaccacgaa cggcgacatc gcgacgctgg tgatggcggt cgatggcgat catccgcacc
     2281 ggcccggcta cgagctgaag ctgaactcgt acgacctctc ggtcgacatc gagctcgctg
     2341 atgcgatcca gcgcctgcgc ttcgagcatc ccgaagtgaa gtgcgtcgtc atcacggcgg
     2401 atctcgaccg cgtgttctgc tcgggcgcga acatctacat gctcgggcag tcgacgcact
     2461 cgttcaaagt gaacttctgc aagttcacga acgagacgcg gctctacctc gaggacgcct
     2521 ccgcccaatc cggcctcaca tcgctcgcag cctgcaaggg cacgaccgcc ggcggcgggt
     2581 acgagctcgc gctcgcgtgt gacgagacga tgctcgtcga cgatggcagc tcggccgtca
     2641 gcttcccaga gacgccactc ctcgcggtgc tgcctggcac cggcggcctc acgcgcctcg
     2701 tcgacaagcg caaagtccgt cgcgatcgcg cagacgtctt ctgcaccgtc gcggaaggca
     2761 tcaagggcaa gcgtgcgaag gactggggcc tcgtcgatca tctcgtgtcg cgcacgaagt
     2821 gggacgagac cgtcgcgcaa cgcgcgaagg agctcgccgc gaagcagcaa gtgacgcgcg
     2881 gtcccgcatt cgtgctcgcg ccgctcgagg cgaaggtcac cgcgaccaag ctgacgtatc
     2941 gcttcgtcga attcaccgtc gacgcggccg ctcgcaccgg caacctcctc atcaaaggcc
     3001 ccgacgccgt cgacaccttc gacgggaacg ccgagccgtg gtcgctccgc gcattccgcg
     3061 agctcgacga cgcgctgctt cacctgcgct tcgactatcc cgagatcaac gtcgtcaccg
     3121 ttcgcacgag cggtaagcaa gacatcgtcc gcgactgcga cgaggccctc gcaaaggcga
     3181 gctcgggctt cgcgcgcgag atccggctgc tacagcgccg cgtgctcaag cgctacgaca
     3241 acacggcgcg cagcctctac gccgtcgccg acaacaccga ctcgtgcttc gccggctcgc
     3301 ttctcgagct cgcgctcggc gcagaccgct tctacatgct gatcgacggc gacgagaaga
     3361 tcggcgtcac cacgagcgtc gcgaacagcg gcttcatgac gacgtcgact ggcctctcgc
     3421 gcctcgatgc ccgcttctac ggcgatccat cgcaggtgca gaagatcctc gcgcgcggcg
     3481 cggaaggctt catctcgagc gaggacgccg agaagatcgg catcgcgacc gtcgctgcag
     3541 acgatatcga cttcgaagac gagctgcgca tcgcgatcga ggagcgcgca tcgctctcgc
     3601 cagacgcgct caccggcatg gaagcgtcgt tgcgcttcgt cgggcccgag acgctagaga
     3661 caaaaatctt tggccgtctc agcgcgtggc agaactggat cttcacgcgc ccgaactcca
     3721 ccggccagca cggcgcgctc acgctctacg gccaacccga acgtccgcag ttccagtgga
     3781 agaggaccta acccatgtct gccatcctca acgacgacaa gatcccgaac aacgtcaacc
     3841 tctcgagcga caagcgcctg cagcgcgcgc tcgaagcgtg gcagccgaag ttcatcgact
     3901 ggtggaagga catgggcccg gtcggctggc aggagcgcga catctatctg cgcaccgcga
     3961 cctccgtcga ggcaaacggc tgggctcact tcgactacgt gaagatgcct gactatcgct
     4021 ggggcatctt cctcgagccg aaagacccca atcgcgtgca cggcttcggc gacatggaag
     4081 gccagcccgt ctgggacgaa gtgcccggtg agttccgcaa catgctgcgc cgtcttgtcg
     4141 tcacgcaagg cgacaccgag ccggcctccg tcgagcagca gcgcaacctc ggcgcaaccg
     4201 caccgtcgct ctacgatctt cgcaacctct tccaggtcaa cgtagaggaa ggccgccacc
     4261 tctgggcgat ggtctatctc ctccacacgt acttcggccg cgacggtcgc gaggaagcgg
     4321 aagagctcct tcagcgtcgc tcgggcgatg ccgacaagcc gcgcatcctc ggcgcgttca
     4381 acgagccgac gtcgcattgg ctctcgttct tcatgttcac gatgttcacg gatcgcgacg
     4441 gcaagtatca gctcgccgcg ctcgccgaat ccggcttcga tccgctcgca cgcacatgcc
     4501 gcttcatgct caccgaagaa gcgcatcaca tgttcgtcgg agagacgggc gtgcagcgcg
     4561 tcgtgaagcg cgcatgcgag ctgatgaagc aagaaccgaa cggcgacgtc cgcaagctcg
     4621 gcggtgttga cctgccaacg atccagaaat acctccacct ctggtacacg ctgtcgctcg
     4681 acctgttcgg cggcgaggtc tcctcgaacg cagccgatgc attcgccgcg ggcctgaagg
     4741 gccgctacaa ggaagaccgc taccccgagc acaagctaat cggctcgaca ttcgcgatgg
     4801 acgtcctcga agacggcaag gtcgtgcaca aagacatcgc aatgcgcaac gcgctcaacg
     4861 aggtcctgcg caccgagtac atcaaggact gccagcgcgg cgtcgatcgc tggaacaaga
     4921 cgatcaaaga cgaaggcatc gacttcgagc tcaagctccc gtcgaacaag ttccatcgtc
     4981 agatcggtat gtggagcaac catcgcttct cgccgagcgg cgacatgctc gaccaagcaa
     5041 cgtgggacgc caagcacggc gagtggctgc caaacgccgc cgacgagacg tacatcaaga
     5101 gcctcatgaa ggcgaactac gagcgaggca agatggcgaa ctggatcgcg ccgccgctca
     5161 aaggcatcaa cggccttccc gaagacttcg agtacgtgaa gctctagttc gcgtcagcgt
     5221 cttcggcgca ggtccaagcc gggcgccgtg gacggctcac atgccgtgaa atcaacggtg
     5281 catcgaaagc cggcgccgca cgtccacgca tcgcggttcg gctgaagtcg cacgcaggct
     5341 cgctcgcagt gatcgagtcg ccagacgtgg ccgatgtcgc actcagccgg gaccaagggt
     5401 cgtgccgcca cgacgggctc gggatcgggc gccgttgcga gtccgacagc gagagtgatg
     5461 ccacccgcca gcacgagcag aagtccgatc ccgaggaccg tgggtcccga acggtctccg
     5521 cgatcgatcg acgacgcgct tccgaggatc atgacggagc cgatgaccac gcctccagtt
     5581 cccgtcgcga ttctcggcgt tcggtgcgtg catgccgacg agaccacgca cgctatcgcc
     5641 atgatcgacg ctccccgcat ggatcgagca tgacagtgcg acccacggat tgatatgacc
     5701 cgttggggtg cagcgcgagg cgccgattac tctgatttct tgtcgttcgg atcgatgtag
     5761 ccggcggggc gaatcgacgc gggcagcggc gcggggatgc gcttctcata gtcgatccac
     5821 acgcgcgcgt cttcgcggca gtcgtacgtg cggcatgttg ccgggcgttg gtcgtagatc
     5881 gtgcacgcgc cttcggcgtc catgcacgcg cagcgcttcg tcgtcggatc gcgcggcagc
     5941 aggta
//
LOCUS       DATLBZ010000089         3495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10470118, whole genome shotgun sequence.
ACCESSION   DATLBZ010000089 DATLBZ010000000
VERSION     DATLBZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10470118"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VMZ53_04705"
     CDS             <1..309
                     /locus_tag="VMZ53_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27779.1"
                     /translation="VRWRGGYLEVRAELSGEDVELRFDPADHIALPRVFIDGKFFCDT
                     VPLDRLANMHRQRRRVAGDPDPRVSATGLDPLALIEDEHYRRTRLVTDDDHDDHDKEI
                     "
     gene            312..1133
                     /locus_tag="VMZ53_04710"
     CDS             312..1133
                     /locus_tag="VMZ53_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS27780.1"
                     /translation="MSSDKTRFDLSAYPLPKNAQGKTFFDKSPQYGRVKRAFRQLVED
                     PGLGVLTSEPGVGKTAALRNLCAELPRPDHLVLYFCDTSVSPLELYRSLASELGVRPS
                     HRRGQLWADIKKALVHMVDERGSHPLVVLDEAQHLSDRFLLDLAGFLNFAFDSRDLLT
                     LWLVGQPALSRRLHMHQHAALRTRIAIELRLEALDREAFAAAVEHGLKAAGASQKILA
                     DAAMEMLFRGSRGVLRTASKILRIAFRMAADRGQSFLDEPVVQAALDELGVGVAA"
     gene            1130..2104
                     /locus_tag="VMZ53_04715"
     CDS             1130..2104
                     /locus_tag="VMZ53_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS27781.1"
                     /translation="MTVADEDALPIVHAAGLDDGDALPHWLVDTLWARAGVGVLGGAP
                     KSCKSWLALDLAVSVASGTPCLGVFAVDDPGPVLLYMAEDAAPIVKARLAGICHARQL
                     ELATVPIHVITAPVLRLDRGRDQARLRAAIRGLAPRLLVLDPFVRLHRIDENDAGQVS
                     ALLGYLRTLQRQHDVAVLVVHHARKNGGSGGLSLRGSGDFYAWGDCYLYLRRHKAALV
                     LVLSIEHRAAAAPDPIRIELVTDDVIDTHLALVPDGAVPQDHDALDDFDRRVLDALRR
                     AGHPVTRSQLRQDLRVRNERLGDTLARLAAAGSIVRDGDRWAVPVPSL"
     gene            complement(2234..2644)
                     /locus_tag="VMZ53_04720"
     CDS             complement(2234..2644)
                     /locus_tag="VMZ53_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27782.1"
                     /translation="MAENVPEDIVSLSSGLDRMSVRMSEPADPTTAPTPQRTEPYERT
                     PPPPQEHHYRKAWVGSTTAYDAEGKELRTWRYAVEADADPTKLADRVAKDVAWLLKAN
                     PLVPVHCVQDAAPELQRLPEALARVLLDRLANDT"
     gene            2672..>3495
                     /locus_tag="VMZ53_04725"
     CDS             2672..>3495
                     /locus_tag="VMZ53_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27783.1"
                     /translation="MVRDLDAHRRRAFRDESSTWRDQAHGAERSFDATATSIGAQLAH
                     DARHVWRHVLNDPDAQLDRFDRRTVVHANLAVKVLVDFVAAPSAVVSRRRCLLHPAVV
                     PSLDHHFVRVRDRFAEISILAVPQRVAAQRRRHLLAFLEGRVFPEAFANRAIDHFREL
                     ERDFIHHPRRRRCHGQPPSLDDFAAASRIANSAITSASMVAAAASRSSKVEVGPSLMA
                     ISRSIMFAISTACGLTVLCLRRISPTLILLAPSFKPVAPNHRSSSSHDQRSGLDPP"
BASE COUNT          512 a         1272 c         1031 g          680 t
ORIGIN      
        1 gtgcggtggc gcggtggtta cctcgaggtg cgcgccgagc tcagtggcga ggacgtcgag
       61 ctgcgcttcg accccgccga ccacatcgcg ctgccgcgcg tgttcatcga cggcaagttt
      121 ttctgcgaca ccgtgccgct cgaccgcctg gccaacatgc atcgccagcg tcgtcgcgtc
      181 gcgggtgatc ccgatccgcg cgtgagcgcc accggactcg atccgctcgc gctcatcgag
      241 gacgagcact accggcgcac acgcctcgtc accgatgacg accacgacga tcacgacaag
      301 gagatctgac catgagcagc gacaagactc gattcgatct atccgcgtac ccgttgccca
      361 agaatgccca gggcaagacg ttcttcgaca agagcccgca gtacggccgc gtcaagcgcg
      421 cgttccgcca gctcgtcgag gatcccggtc ttggcgtcct gacgtcggag ccaggcgtcg
      481 gcaagaccgc cgcacttcgt aacctgtgcg ctgagcttcc tcgccccgat cacctcgtcc
      541 tctacttctg cgatacctcg gtatcgccgc tcgagctcta ccgttcgctc gccagcgagc
      601 tcggcgtccg gccctcgcat cgccgtggcc agttgtgggc tgacatcaag aaggcactcg
      661 tgcacatggt cgacgagcgc ggcagtcatc cgctcgtcgt cctcgacgaa gcgcagcacc
      721 tatccgatcg gttcctgctc gatctcgccg gctttctcaa cttcgccttc gacagccgcg
      781 acctgttgac gctctggctt gtcggtcagc ccgcgctctc gcggcggctt cacatgcacc
      841 agcacgctgc gctccgtact cgcatcgcca tcgagcttcg cctcgaggcg ctcgatcgcg
      901 aggcgttcgc tgccgctgtc gagcacggcc tcaaagccgc cggcgcgtcg caaaagatcc
      961 tcgccgatgc tgcgatggag atgctgttcc gcggcagccg cggcgtgctt cgtactgcct
     1021 ccaagatcct gcgcatcgcc ttccgcatgg ctgcggatcg tggtcagagc tttctcgacg
     1081 agcccgtcgt ccaggcagcg ctcgacgagc tcggtgtcgg agtcgccgcg tgaccgtcgc
     1141 cgacgaggac gcgttgccga tcgtgcatgc cgccggtctc gacgacggcg acgcgcttcc
     1201 acactggctt gtcgacacgc tgtgggcgcg cgctggtgtc ggcgtcctgg gtggcgcgcc
     1261 caagtcctgc aaatcgtggc tcgcactcga tctcgcggtc tcggtcgcct ccggcacgcc
     1321 gtgcctcggt gtgttcgccg tcgacgatcc gggcccggtc ctgctctaca tggccgagga
     1381 tgccgcgccg atcgtcaagg ctcggctcgc cggcatctgc catgcgcgcc agctcgagct
     1441 tgccacggtt ccgatccacg tcatcaccgc acccgtcctc cggctcgatc gcggacgcga
     1501 tcaggctcgg ctacgcgccg ccatccgcgg cctcgcgccg cgcttgctcg tgctcgatcc
     1561 gttcgtgcgt cttcaccgca tcgacgagaa cgacgcaggt caagtctcgg cgctgctcgg
     1621 ctacctccgc accctgcagc gccaacatga cgtcgccgtc ctcgtcgtcc atcacgcgcg
     1681 caagaacggc ggcagcggtg ggctttcgct ccgtggcagc ggcgatttct acgcgtgggg
     1741 ggattgctat ctctacctcc gccgccacaa ggccgccctc gtgctcgtgc tctcgatcga
     1801 gcatcgcgct gccgcagcgc ccgatccaat ccgcatcgag ctcgtcaccg acgacgtcat
     1861 cgacacccat ctcgcgctcg tgcctgacgg cgcagtcccc caagaccacg acgctctcga
     1921 cgacttcgat cgccgcgtgc tcgatgcact ccgccgtgcc ggtcatccgg tcacacgttc
     1981 gcagcttcgc caagaccttc gcgtacgcaa cgaacgcctc ggtgacacgc tcgctcggct
     2041 cgccgccgcc ggctccatcg ttcgcgacgg cgatcgctgg gccgttcccg ttccctctct
     2101 ctagccacac gccggaacgg aacgaccgcg acctatcccg cggcgacgtg gcggctggcc
     2161 tgcaagatcc ctaagcgtcc gccacgccgc cgcacttcgt tccatcaaac cccgacgaga
     2221 acgtgggacg ctctcacgtg tcgttcgcga gccggtccag taggaccctg gcgagagctt
     2281 caggcagccg ctgcagctcg ggcgctgcat cctggacaca gtgaaccggg acgagcgggt
     2341 tcgccttcaa gagccacgct acgtctttgg caacacggtc ggcgagcttg gtcggatcgg
     2401 catcggcctc taccgcgtac cgccacgtgc gcagttcttt gccctcggcg tcgtatgccg
     2461 tcgtgctgcc aacccatgcc ttgcgatagt ggtgctcctg cggcggtggt ggcgttcgct
     2521 catacggctc ggttcgctgt ggtgttggag ccgtggttgg atctgcgggc tcactcattc
     2581 tcaccgacat tcggtcgagg ccgctgctga gcgacacgat gtcctcgggc acgttctcag
     2641 ccattcgcgc aacatcttcg agcgccgctg cttggtccgc gatctcgatg cccatcgccg
     2701 tcgtgcgttt cgcgacgaaa gctcgacttg gcgggaccag gcccacggcg cggagcgttc
     2761 gttcgacgcc acggctactt cgatcggcgc tcagctcgcc cacgacgcgc gccatgtctg
     2821 gcgtcatgtg ctcaatgatc ccgacgcgca gctcgatcgg tttgatcgtc ggaccgttgt
     2881 gcacgccaac ctcgcggtaa aggtcctcgt cgatttcgtg gctgccccat cggccgtggt
     2941 aagtcgccga cgatgcttgc tccatccggc ggtagtgccg tccctcgacc accacttcgt
     3001 ccgcgtccgt gatcgcttcg cggagatctc gatcctcgcg gtgccacaac gagtcgctgc
     3061 tcaacgccgc cgccatctct tggcgttcct cgaaggtcga gtttttcccg aggcgttcgc
     3121 gaatcgcgcg atcgatcact tccgagagtt ggagcgcgac ttcatccacc acccgcgtag
     3181 acgtcggtgc cacggtcagc ccccatcctt ggacgatttc gcagccgctt cacgcattgc
     3241 gaattccgcc atcacctcgg cttcgatggt cgcggcagca gcctcgcgct cctcaaaggt
     3301 cgaggtcggt ccgagcctta tggcgatctc acggtcgatc atgttcgcga tctcaaccgc
     3361 gtgcgggctg acggttcttt gcttgcgtcg catctcaccg acgctgatcc tcctcgcgcc
     3421 atctttcaag cccgtcgctc caaaccatcg aagttcaagc agtcacgacc agcgctcagg
     3481 actggatccg ccttg
//
LOCUS       DATLBZ010000090        32248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6744647, whole genome shotgun sequence.
ACCESSION   DATLBZ010000090 DATLBZ010000000
VERSION     DATLBZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 32248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32248
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6744647"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VMZ53_04730"
     CDS             <1..556
                     /locus_tag="VMZ53_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HUS27784.1"
                     /translation="SAAPREKPADFVDVPTLIPDAVLDIRYATDDNFTGKAVYPVAHC
                     KLRRSVAARLVKAAALLRKSDRRLLIWDCYRPKSIQEVFWKLVPDERYVADPKKGSRH
                     SRGAAVDVGLVDKDGKTVTLPTKFDDFTEAAHRKNALKGEAGAEARTLEAAMKNAGFI
                     GMPTEWWHFDAPDAAKFPLSDEAL"
     gene            complement(553..1038)
                     /locus_tag="VMZ53_04735"
     CDS             complement(553..1038)
                     /locus_tag="VMZ53_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27785.1"
                     /translation="MKLALALVALAACGGSSKPTDSPSSHSPTASEVTATEVTNASTG
                     PTTSPTSMSSSTAQPAAAKKQPYSCFAYSQRGSTVKRSACMRTEECGPYLEQARQVGG
                     IVADAGCASVDVLFCFHQAPTKADPQGLDVCQPTVDACKSERALLVKNGTSVDSDCAL
                     R"
     gene            complement(1048..1968)
                     /locus_tag="VMZ53_04740"
     CDS             complement(1048..1968)
                     /locus_tag="VMZ53_04740"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HUS27786.1"
                     /translation="MDRPSIRQLECLVAVADQHSFRKAAAALGISQPALSAQVQCVEK
                     LFGVQVFERDRRSVLVTPAGEDIVGRSRLALEAIDAVSDAARRRAEPLVGPLRLGVIP
                     TVAPYWLPALLPTVRKRFPKLELILREDQTARLLGQLAAGLIDAALLAIPVPGDVTTA
                     AIARESFLLAAPRDAAIVHKRGKLTEKDLEDQTVLLLEDGHCLRDQALAVCERSGAVE
                     SMEVRGTSLPTLVQMVAGGMGVTLIPEAAADALVQPRGPVDLAEFTKPPGRTLGLAWR
                     TSSARLREFRLLAEVMSETADKFLAKLRTK"
     gene            2042..2581
                     /locus_tag="VMZ53_04745"
     CDS             2042..2581
                     /locus_tag="VMZ53_04745"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HUS27787.1"
                     /translation="MLTIGDTFPQFKATAVTGLEPGKQFTDVTDASYAGKWKVVFFWP
                     KDFTFVCPTEIAAFGKLERDFRDREAQLLGVSIDSEYVHLAWRNNHPDLKDLPFPMIA
                     DIKRELSSSLGVLHKEDGVAMRATFIVDPNNVIRFVEVTDMNVGRNPQETLRVLDALQ
                     TDELCPCNWQKGQNTLSAA"
     gene            2592..3152
                     /locus_tag="VMZ53_04750"
     CDS             2592..3152
                     /locus_tag="VMZ53_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUS27788.1"
                     /translation="MTTNLDQLKERLPDYARDLRINLGVLTSSTALTPAQAWTIALTS
                     AVRTGQRDLIAAIEGDGQLAPEADFRARAAAAIMGMNNVYYRFLHFMGEASEYGHMPA
                     RLRMQVIGNPGPKTEGAGFTPATEADKLDFELACLAASAITGCESCVRSHEKAVREKG
                     GSKEMVQDAVRIAAVVNAIAIALPSS"
     gene            3279..3773
                     /locus_tag="VMZ53_04755"
     CDS             3279..3773
                     /locus_tag="VMZ53_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27789.1"
                     /translation="MDRTLSHGEPSKPLLKVLPIKRWIPQDIHSMMDYADGLTAASGA
                     MMADEEDEVAFWASIALGASSIGVSAMTDYRLSVAKIIPIKAHEAIDYMWGMSCIAAP
                     FALGYWKSSPRTALAHVMVGAGTILASMFTDYRSYTEERAKRRASSGGGNAGVKVRRR
                     SQAQ"
     gene            complement(3777..4718)
                     /locus_tag="VMZ53_04760"
     CDS             complement(3777..4718)
                     /locus_tag="VMZ53_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27790.1"
                     /translation="MTWPFSQGGGLLALYLATLALHAVFIGYVVAGTAFVLIRRDDPI
                     AATVRDRLPFMLGAGITAGVAPLLFVQLLYQHRFYTANLLLGPRWMAVVPVLIVGFYG
                     LYIQKVSEKWRRPASIVSLACFVFVGWSWSELHELMKADPVWHDFYAAGQRVFFAGTI
                     APRSAVLFPAMATSFAMIAAWSADRDGRQRLALLAMGSRVVSIGGAVWLWRAGFSLDG
                     AALAWFGVLCAAVVVELGAWAVIWRRPSDGALAVATAAGTSALVAATVVREAPRIPLL
                     EPARDIADQAGGSLVFAAALVIGVVAIAWVVKTARLA"
     gene            complement(4715..6130)
                     /locus_tag="VMZ53_04765"
     CDS             complement(4715..6130)
                     /locus_tag="VMZ53_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS27791.1"
                     /translation="MDSIPFYPVNDFGPLMKGMVIGGVGILHVFLAQFAIGGGMLLLY
                     FERLAQQGKEKDARAFVDGYFQILVLISFVIGALTGVAMWFTTIQVGARTIGLMVDQF
                     HWFWATEWVAFSVEVAAGYAFVRVGRRLNDRARIRLLALYSAASWFSLFWINGILSWQ
                     LTPGRYTDGHSVWAGFFNPSFWPSLIFRTAVAMALAALAACIIINTLDLDRERRAALI
                     RRAARFTAPMALMPVIALWYLAVIPADSRSWLMGGSMAMTMFVAIAAGASLLIGGYVV
                     VGLLYKRLYINGATATLLLALAFGATAAGEFVREGARKPYTVRGILYSNAITPDEVAH
                     LRVTGAATNDPYPLRDASRFPTEQLRRGARVHRVLCDACHTLHGANGLVELTRTWTDD
                     QMRVNIAKLQRTKGFMPPFAGNAEDVEAIVQLLRWERADAPRAWPESTDPAMLTQINA
                     WLDEAGTAQAPPPTSNAEATR"
     gene            6226..8178
                     /locus_tag="VMZ53_04770"
     CDS             6226..8178
                     /locus_tag="VMZ53_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27792.1"
                     /translation="MRSVLIASLAVFAAACGDKDLGTPDACNPLGGQACITPWPSSVY
                     ESEDTTTETKMKVDIPAGALPKNFDNIELSPALYNKQDGFSYAAPMIAAFPTGVDPAN
                     LVDNRNIGMSITDASPTVLIDMSTGELVPHFAEVDTPAAATPESQALFIRPAKLLKPG
                     TRYAVAIKSTLKAKGGGALPISEGFQAILDDEETSHPLLEKVRPRYKDIFAALEAKGI
                     HKTDLVLAWDFTTRSHADVQSDLLNARTAALAMIGDASALHFTIDTDTTPGDTRIARQ
                     ITGTFDAPLFLDTEVTTASQINLLRDGSGKPMASKLYRVPFTAIIPQCALQGGETVPI
                     MLYGHGLLGASNQVASGGTRAAAAEICAVAVGTDLRGMSEVDVPNVALALNDGNDGHL
                     IFDTLIQGMMNHIALVQIAKGPMAAAGSGLFRKNGDQPLIDPDKFYYYGISQGGIMGT
                     TICGIDPVIKKCVVQVNGINYSMLLERSADWPQYAVILQGGYGNPLVTALMINLMQNE
                     WDRTEPVAVADVLTTTGFPNTPAKQVFMQFGIADDQVPNVGSELQMRTMGIPLITPTP
                     FHIYGVEEKTSAPSGAVMYDFGLSGTIPLTNTPPPRNDVHSNIRNKKATTDMMKTFYE
                     TGVITNVCTAPNGCVCTVAGACGPGV"
     gene            8194..8670
                     /locus_tag="VMZ53_04775"
     CDS             8194..8670
                     /locus_tag="VMZ53_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27793.1"
                     /translation="MRAALPFALVIVAACLPPDAPPQAPVAPGQGKLVTFPCESEFTR
                     ADAQITDHLRRARLWNFAEPTQSKVSIEVVQLAIECTDPTLDCYAAVGKSVHADAILF
                     ATIDRASEDGALVVTVSVFDVRANAWVRRSAKKFPTEDDALYELKDIVADATKRGP"
     gene            complement(8674..9702)
                     /locus_tag="VMZ53_04780"
     CDS             complement(8674..9702)
                     /locus_tag="VMZ53_04780"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HUS27794.1"
                     /translation="MSTLRKVLFVSLPLAAAGALWFGHQTQPASAADRQTIVRPAILV
                     APGRVETVRDPVALAFETGGRIVSIDVDEGQAVKAGQVVARLDDRMAAARVASARAAV
                     AGAQASYLMSKRGARTEDLDAAKAELEAAQAAADHSASERSRSEKLGEKGAISSSIVD
                     ADSAAARVSSAQAAAAQARYNALVKGSRSEQVAAAAASLDATKAELAAAEVALDQTVL
                     RAPHDGVVLRRLAEVGALVTTVNPSTIITVADLRTLEIRAELDEADVAAISVGMPAYA
                     TADAFGERRFPVRISRVTNELGRKTVRDDDPRARVDTRVLEVIAQFDGTPSESLPLGL
                     RMYVHVER"
     gene            complement(9686..10396)
                     /locus_tag="VMZ53_04785"
     CDS             complement(9686..10396)
                     /locus_tag="VMZ53_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS27795.1"
                     /translation="MQDSLAKLVRTGKTYGAGETAVAALSDATLDIRAGEVTLIEGPS
                     GSGKTTLISILGLLLKPTTGEVWLEGRNVAALSERELPGLRARNFGFVFQGFNLFPAL
                     TALDNVAIAIQMKDPKAKSPRSEAARLLEMVGLGSRAHHLPGDLSGGQKQRVAIARAL
                     GGNPPIIIGDEPTAALDTKTALSVMELLRELATQRGRAVVVVTHDPRLERFADRVVRV
                     EDGHIASIDQGAHHVHAA"
     gene            complement(10399..11532)
                     /locus_tag="VMZ53_04790"
     CDS             complement(10399..11532)
                     /locus_tag="VMZ53_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUS27796.1"
                     /translation="MWSLARKLLFHDRLRFAVAIAGVSVSVMLVLVQVGLYFGFMNTA
                     SSIIDSSHADLWVGKEGSESFEFASPFDERTFYKVASVPGVERTERVLMNFAQFKLAN
                     GGDLGVQIVGIETSPGHTPMLAPWNVIAGDANHLRDAGAIVVDRSEYAKLKIDHVGHT
                     TEVSGVHAEVVAMTTGIRSFTTSPIVFADIRTARSFMPGLGENAVTYVLVKVKPGENV
                     IDVKARINALPHLAAYTTSEMSKRTRTYWSSRTGVGAGFFTTAVLGIIVGFVVVGQIL
                     YSGTLQYIREYGTLKAMGAKNSMVVRVILSQAMISAAIGFVFGGVLAFGMQAAMAKAN
                     LTVALFPGLYVATLIVTIAMCSFASLLSIIKVLRLDPASVFKG"
     gene            complement(11642..12793)
                     /locus_tag="VMZ53_04795"
     CDS             complement(11642..12793)
                     /locus_tag="VMZ53_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27797.1"
                     /translation="MAPRIAALVFALATGCRAVFGLEPPAHDDDAFDDAAIIDDAPLD
                     ACPGMSTVFSTCMLAFETDVDLTGIIIYNTDTRTASGPGGTGSVQLASVEMPTATGAV
                     VVLPFRSFTLASGALLRIVGSKPIAIVAEDHVDILGVLDVGAAGASHRDDCDSIDGVS
                     GVGGGGGGGGGGFASIGGNGADGNNDATPRAGGNGGTAQPRPPGPIGGCDGGAGGAGS
                     GNAGTTGKGGGALMLVSQTVTISTDGRVDATGGGGGGGKVADGGGGGGGSGGFILIEA
                     STLQIAGTLVANGGGGGEGGDVLTAGDPGVRGTSTTAAAAGGAGNSAEGGDGGAGGAA
                     LTLAGRSPNQLKNGGGGGGGGGVGFISVRAPQLTVTSSGVISPALQVWP"
     gene            complement(12814..14706)
                     /locus_tag="VMZ53_04800"
     CDS             complement(12814..14706)
                     /locus_tag="VMZ53_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS27798.1"
                     /translation="MLIAGLLLILIAAVGAAYGGIVIRAAPKRRDNVMFGLLAMTDAG
                     MILWRAITVLTGGSITSESVTLPCGLGTMAMAVITMDFLHSFPRRAPMKWRWRLLLIA
                     WAISGALVTALVDAGKPWNVLRVSEFTFYLPATLVVFFIGARAWKRTKDRDARTVIAM
                     LWFRWGFGFCAYTFGSLLGFFEEALWAETTFATLTSFVVIGTAVLRTELFSIRSAAAE
                     VVVATTIAMIVAISGGAAVFAVLRWTDAGSALQTAMLVGATLVPLALAAVGRAMYPRL
                     EKNVLAGLDERRARRLGVQGEPLPAAPEAAIAEACKRITSMGDGATVTWQAAAQMSPE
                     LSQTLKSGDLQRKDETSELPACLIVPALGADSTLVGAFMIEHGTLDRDTYVVARDLAA
                     RIALVVERAQAVSELDDARRLAALGQFAAAIAHDIRTPLTSISLNVQILRQKLQLSDD
                     DREHLDIALEELARLDKSVAEILDFAKPLKLAPQSIDVGELIETTARGLTPVLSEKGI
                     ALSCDAAPDELPAVQGDPQRLRQVLVNLVGNAAEASTRGAQVTVRAKPADATHVAIEV
                     EDRGRGIKADDLPRIFEPFFTTRPDGTGLGLAICHKVVRAHGGELQVRSIVGEGSTFT
                     ILLPAA"
     gene            complement(14777..16168)
                     /locus_tag="VMZ53_04805"
     CDS             complement(14777..16168)
                     /locus_tag="VMZ53_04805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS27799.1"
                     /translation="MARILIVDDDRSIRRTLEKFLAGEGYEVATAQDAPGAIAAVQRG
                     EPVAGQRGPFDLMLLDLGLPGGSGFDVLSAIAGRDDAPTIVVVTARDDMQSTVKAIQL
                     GAYEYLVKPVDIDRLRTVVKTALDSREAREQLVELTARGPEAGDILGKSAAIRDVWKQ
                     IGAVSTTRAPVLIRGESGTGKELVARGIHMASEAGREGRPFVAVNCTALAPGVLESEL
                     FGHVKGAFTGAVADRPGRFELAGKGTIFLDEIAEIPLELQAKLLRVLQERTYERVGDA
                     KTMQHEARVIAATHRDLSQMVAKGTFREDLYYRLRVVEIALPSLRERASDIPILVEGL
                     LAKINKDLGKEVRYVTPAALAKLSAYAWPGNVRELENTLTRAVVLAKSDVLDETLLPL
                     GSSAQPLAADVDAEGSLMTLREIERRHIARVLAHTEWNKRRACAVLDISRPTLDRKIE
                     EYGLVKPTGGDES"
     gene            16317..17027
                     /locus_tag="VMZ53_04810"
     CDS             16317..17027
                     /locus_tag="VMZ53_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUS27800.1"
                     /translation="MQTDSLEELWPLGDLVAEAANRLEALPAPKNGQVRAVPDERTVR
                     YYAAQGLLDRPAAMRGRTALYNRRHLAQIVAIKRMQQAGHSLADIQKMWATVEDSTLT
                     RISGVLMTSTVNPAGGPMRTNPKSPRKDFWKQLPEILPTPTVVTPLPRTRGTTPLPPT
                     PGTTPLPPLTRGTQAAELRVEVAPGVAVVVALPTERSISISPADVHAVRVAAAAVVTE
                     LANRGLLARGTGTLEEEP"
     gene            17042..19651
                     /locus_tag="VMZ53_04815"
     CDS             17042..19651
                     /locus_tag="VMZ53_04815"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HUS27801.1"
                     /translation="MSEEEVAALGRNRDENAGFGCLRTPQGCVPMSAMDVDARVVGVM
                     AAVELAQTYVNTTATPIEATYIFPMPDRMAVHRFRMEVAGRVIEGVVDERGAAREHYE
                     QAIAAGHRAAITEEERAGVFTLRVGNLMPGEVATIRLSLVGPLPIDDGEVTFRFPLVV
                     APRYMPGAWLGGEQAGLGVAGDTSLVPDASRISPPVRLPHIPNPVRLGIRVALADGHV
                     RDVASSLHAVTSAMRDAQVIELGPSERLDRDFVLRWRLDGDELRSGLVCADDADGKGG
                     TFMLTFVPPSTLAMSQKPRDVVFVIDRSGSMEGWKMQAARRATARMIDTLTSRDRFFV
                     IAFDNAIEVLPDVQLGYATDRMRFKAVEELAKVNARGGTELEQPLSLAVRTLAGGHDD
                     RERVIVLVTDGQVGNEDHILRQVAPNLRNVRVFTLGIDQAVNGAFLRRLAGAGGGLCE
                     LVESEDRLDVVMSKVHRRIGTPVATEMRLDAHGMDIVWNQVAPAKLPDVYAGAPVVVL
                     GRYRGAAALDAAVTVDGTSFGEPMRLRIARESTTGTSALGASWARAYIRDLEDKYATG
                     SRELEGEIVRVSKQWNVLSKFTAFLAVDRSEVVNPGGRLAQAVQPVESPAGWEGAASG
                     AVGGMPSLPGGMPSPMSGGGSLRSRAVMPARAMAAGRPMAAGGKMAPPMDGAFASPPP
                     PPMAPRPTESAPMPAFADLSIDPASPSPAEKSIAAPRPAPRTPGPASNSMSAPPSYRP
                     PMQQAPAKGKREAEPSDALGRSASGDASAYLAKLQSFARELEAEARGAVSVGKLKLLR
                     QRLREWVEDLRSVGGFDSLADVVEQLAHKLGDAIAAADVATQVASLATQLANVAAGGA
                     VPPRSTGRPAFWK"
     gene            19678..20427
                     /locus_tag="VMZ53_04820"
     CDS             19678..20427
                     /locus_tag="VMZ53_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HUS27802.1"
                     /translation="MKLAVFVVVLAGCSRPATPRHDETKLAPLDVAPAVVLEPIDART
                     ADATADAASVSIVDAPIKWSDERERLTLEYRRRHSDPDASDLTIAPKVIVLHYTAGGS
                     AKATRGYFDNTKIEAARKDLARGGAVNVSAHFLVDRDGTIYRLQPETRFARHCIGLNH
                     IAIGIENVGDEAKYPLTDAQVEADAALVRDLTARFPITHLLGHYEVMKFKQHAYFIER
                     EAGYANSKPDPGEKFMQQVRERVGDLKLSGL"
     gene            complement(20438..23269)
                     /locus_tag="VMZ53_04825"
     CDS             complement(20438..23269)
                     /locus_tag="VMZ53_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS27803.1"
                     /translation="MIEGEAEILQSSNKPTVLYGRPGMTIKFVEPDEPSKVIIGELEK
                     ARLAMKVPAPSVPPRPANVPPEPRPTPPAPGGRIDATNALAECVVIGDPSTLKETANA
                     PRAIGGEGSGKAKFIVPSIPSAPPGRAKTPSIPGEPTVNTAPPQPPARELPKPTPIAK
                     EASKPSAAPIAPIAREPSKPGPVAAIAREPSKPTANRDPSLKMTSIGFPAIDKAPAPV
                     KPANALLNTMLGTEAPPNPTKEIPAQAPPENPLLQTQHGVGAPKSAPVAKPVVSDDES
                     TSIGEMPAKKSTDPMPAASPPPSTEAAPSLLPEPPRASKAHKATSIGFPAIRTPFETQ
                     PVGVVPPPSNAPRGEGEPAPPIVAKPRQGMPGPRGKNPTTPPLTPRHPTPAAPLPLVR
                     PPAKPMVADEEEKTDLSAVPPAPKPAALPDEQPMPQVDPFAKTDLGGLVSSRSGGMRA
                     SEIMAAIPTEDWTMTPDASGPTVLPSKEEDGEAGDAGSGPVQVEAKKAPKGPPTGNWT
                     ISLDPEEGWSEPQKLPATAAPPPVVEKPAPPPRQTPRSGNPVTSVASSKALEAVEWEE
                     KPTGIGEAKIEIDPTLMEPLKPMPALDDDDPNDPVAPPPAALAPPPSRQTGPVRAEMI
                     PPPLAPMPPVGTGNLPAAAMMSPTGNMPIPGGGFSGFGTSSGFPAAPGSMHDLGASGS
                     GASPEPSAATARKRMIIVIAAAAVAVVAGFLLVLLIAGGSKKKPGASGKGSAALITKG
                     VGSQGSADRAITMGVPDAAVVEDEGSANDEGSDAGSGAGSQVAIETPEQGSAVAPPPP
                     HDGPCKVDVSSVPTGADIYLAKAKVGTTPFSLELPCGTAATLTLKKARFLATDRTFTP
                     SAVKANKITIKLGKTMFSLKVTSNPSGATITIGGKSAGVTPTTVKVAAFESSAITLSK
                     PGFATDTQRVTPKQNGQAHHVVLKKGGRR"
     gene            complement(23532..23759)
                     /locus_tag="VMZ53_04830"
     CDS             complement(23532..23759)
                     /locus_tag="VMZ53_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27804.1"
                     /translation="MAKAKKGKSGGGGGPKEMLLVSSKVKQMIKDAGCNTAGDALEGL
                     NGYVGWLVHQAARRASENGRKTVRAHDFMIF"
     gene            complement(23865..25394)
                     /locus_tag="VMZ53_04835"
     CDS             complement(23865..25394)
                     /locus_tag="VMZ53_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27805.1"
                     /translation="MRPTTLALVTASLVACGKTAPKKEETKAVENSTVNVAPKKQVPT
                     KELPALAADPGGATGKAVWQAAFGGLGTDVPKGIAVNSAGEAFVTGYFEGETDFGGEG
                     PKLGKRKSAGKSDAFLAKVGADGKLAWAQTFGAAREDVGNAVATRGDKVVVAGSFLDE
                     ITLAGQTKKSAGSDDLYIAAFDKDGNAEWMWTAGGIDSDGVNAIAAMPDGGWVIGGSF
                     TDGLDISVGAKHVAVKSKGGTDALLIKLAADGNTEWVKTFGGRYNDSIRHIGVDANGN
                     IFIQGIFKDVANWGGAELKAGGGSDDDVVLAKYDTNGDHQWSQRFGNAFDEAANGIAV
                     DPSGHVTIVGSFENKGGISFGQGDDHQSLGEADAFVARFDGAGKLEWAKTMGGSKVDV
                     ASGVAADAAGNVVVSGWFEGSVDFGKGAVTTKTPNKDVFVEKLNAKGELVWIQTFGDK
                     DHDQARVLAVDDKGAAYISGLYRFKLSAMEPAIDQIIDAKDPVLSKAPKSDVFVVKLD
                     R"
     gene            25443..25787
                     /locus_tag="VMZ53_04840"
     CDS             25443..25787
                     /locus_tag="VMZ53_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS27806.1"
                     /translation="MLRFLMIVGTVLLAGCPSHTPVAPKSPDDTALRIRVAQAEVKRA
                     DGVAELLALAGDKDVHARELALRGLGRSGDAKAYAALELALRDPEPRVIAAAAAAIGV
                     AASLDDKPLSSS"
     gene            26211..27356
                     /locus_tag="VMZ53_04845"
     CDS             26211..27356
                     /locus_tag="VMZ53_04845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUS27807.1"
                     /translation="MKVEAARALGETDDRAIIAPAGSPWQLCVAAAKPSPATATTVDA
                     IVRCPLPDHLKLPLLADLFDEKRSDATFRRAALRQLLASDDPRVRGAGLTALPKTWKD
                     GDEAAQATIVGTVVSALAAKNQIVAGSAVDAAEQLFDAMGDSHRLRATLEAALVQRAT
                     TESDAELVQSMYALIGKKKIGTGAEACRMGLDLPAAAAKAASECLTALGEPKDATPRI
                     VPPPPVDVASVIGKRVIWHVTTTRGGIDIELRPDVAPWAVAVIVSLTQRGYYDKKEFH
                     RVVPNFVVQGGDPTESGWGGPGFTLPAEPASSLDGPGYVEGGVGMADAGRDTAGSQWF
                     IMHSRAPHLDARYTWVGSVLSGQKVANALQIGDRVESATVELTPLSR"
     gene            27488..29284
                     /locus_tag="VMZ53_04850"
     CDS             27488..29284
                     /locus_tag="VMZ53_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS27808.1"
                     /translation="MSDFEFGGSTVAFQMQGRPIGGSKAASPTAAYLAGSRIAAIDDG
                     ALVIDADSGQLIRTDKAGKQLASLAIGKNAGLLTYDPVGKLAYVADRVGNRVVSVKVG
                     DKKLEVAKEIKTPAEPYGIALSPDRSTVMVTTIADRTLVAFDAAGKEKWRTPLSREPR
                     NIAVSPDGTRALVTYLTTGTVDQVDLLETHRTEHIALSTQQGTRRLSRFNQQQPDSFA
                     RGSFTALFMGEHQAIVPFQRETPVQQIGGGERTGSYGGGFDPPITHQLAFLATNGERT
                     AQITAQIGQHQPRALAWDSAHDALYVAGLGSDSIMQIKNASQGTIAQGTEVNLAQKER
                     CGPDGIAIGADGNLMVWCAFTRSIKRVEIVDAKGELMAMAKTTDGPELVASAMSEKEH
                     QGYVLFHAAQPAVSARGAMACASCHPDNRTDGLSWRIDKQELQTPLLAGRIVGTHPYK
                     WDGGDKDLTASLTSTMKRLGGRGLDKVQTEALAAYLEQLPKPIAPSRDSTQVARGEKL
                     FDSAELGCRSCHDGKAFTDQDVHKFTSVTLPDADTPSLLGLAASAPYYHDGSAATLES
                     LLRDRARVHGMAETAKLNDTQVADLVAFLESL"
     gene            29306..30181
                     /locus_tag="VMZ53_04855"
     CDS             29306..30181
                     /locus_tag="VMZ53_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27809.1"
                     /translation="MWRLLVVLVVAVVSSLVGCGRVSFDALALDSAIDARPDAPIDAA
                     FGPPQLVAVSSSVDEEDPSLTDDMLEMYFDANPGLVRVTRTAIDQPWSSRVLVMELGT
                     GSTVNNAKISGDGLALYFTSRRVPSDAADIWYATRPDRTSLFSAPQLVGGVQTSSDEY
                     EPMAVGDGHVIYFSRAILTESLWRASRASTMDPFADTTNIPELDSPAYEGGIWVSADE
                     RVAYFHSDRVSSAERMIFRASRADASDPFGTPVAVSEIDLPGKLEDPWLSPDGHTLYF
                     ASDTSGQYDIYMATR"
     gene            complement(30178..31305)
                     /locus_tag="VMZ53_04860"
     CDS             complement(30178..31305)
                     /locus_tag="VMZ53_04860"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HUS27810.1"
                     /translation="MTWVVPVELEDAVYAATVDVIGDEPLQTAALTRAIVDRSKRYTS
                     DRGHLAKPADKTADLAARAAFFTVADAMKIAIPLGELAGRNALPARRPLRIVDVGAGC
                     GAMSLGIVAMLATDATGEAAFEMTLIDRDVDALRIAAAALRELATKRGVIAGVTTRVA
                     DVETTSLPHGDLVVMGTVLNELAMPARVKLVEGALAATAADGAVIIIEPALRDTSRAL
                     HELRDAIIGIGKANVFAPCTRKLAPCPMLANADDWCHEDRLVQLPPRTAELARLTHLR
                     DSGLKFSYLVLRHDAAPLVDAPGAWRVVSSAFSAKGKHEMFGCSDAGRVPLRLLKRNR
                     TEANKAFERARRGDVVVTDVAVADDRVELAGTVERREPAGR"
     gene            complement(31302..32183)
                     /locus_tag="VMZ53_04865"
     CDS             complement(31302..32183)
                     /locus_tag="VMZ53_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27811.1"
                     /translation="MNVRSSNVLIVLALLCACSGKTKGVEDARKHPQDSAPVTDPTAP
                     PPSGKGDVQLRVEWKDVPLAARASSGRTPCNTPRAASVAPTTTWGIPDVFVMIDAPPA
                     TTAAALSKSRGSNATGSATTAPVMTAAAMTREARVVLDHCALTPRVALASDKLIVASA
                     AESPAKVVITKAGAAFSDSAGTATSAGALTAYLPIAGHSIEVGVDAKAAYHVEIVGED
                     GKSRDAEDAWVVVADSPFVAITEASGQVLLRDVPAGTYTATAWLPPRAGQPAKIAHAQ
                     VVVTDGGLADVTLDISK"
BASE COUNT         5494 a        10623 c        10817 g         5314 t
ORIGIN      
        1 ctcggcggcg ccgcgcgaga agccggcgga cttcgtcgac gtcccgacgc tcattccaga
       61 tgcggtgctc gacatccgct acgccaccga cgacaacttc accggcaagg ccgtctatcc
      121 ggtcgcgcat tgcaagctac ggcgctcggt cgctgcgcgt ctcgtgaaag cggcggcgct
      181 cctacgcaag agcgatcggc ggctgctgat ctgggactgc tatcgcccga aatcgatcca
      241 ggaagtgttc tggaagctcg tgccggacga gcgctacgta gcggatccga agaagggctc
      301 gcggcacagc cgtggtgctg ccgtcgatgt cgggctcgtc gataaagacg gcaagaccgt
      361 gacgctgccg acgaagttcg acgacttcac ggaggccgcg catcgcaaga acgccctgaa
      421 gggcgaggcc ggcgcagaag cgcgcacgct cgaggccgcg atgaagaatg ccggcttcat
      481 cgggatgccg acggagtggt ggcacttcga cgcaccggat gccgcgaagt ttccgctctc
      541 cgacgaggcg ctctagcgca gcgcgcagtc gctgtcgacc gacgtgccgt tcttcaccag
      601 tagcgcacgc tcggacttgc aggcgtcgac cgtcggctgg cagacgtcga gtccttgcgg
      661 atccgccttc gtcggcgcct ggtgaaagca gaacagaacg tcgacggatg cacagcccgc
      721 gtccgcgacg atgcctccga cttgccgtgc ttgctcgagg tagggcccgc actcctcggt
      781 gcgcatgcac gcgcttcgct tcacggtgct gccgcgctgc gaatacgcaa agcagctgta
      841 cggttgcttc ttcgcggcgg ccggctgcgc ggtgctcgag gacatcgacg taggcgacgt
      901 ggtcgggccc gtgctcgcgt tggtgacctc ggtcgcggtg acctcgctcg cggtcggcga
      961 gtgcgacgac ggcgagtccg tgggcttcga ggagccacca catgcagcga gggcaacgag
     1021 cgcgagcgcg agcttcatgc gggcatctta cttcgtgcgc agcttcgcga ggaacttgtc
     1081 ggccgtctcg ctcatcacct cggcgagcag gcggaattcg cgcagccttg ccgacgatgt
     1141 tcgccatgcg aggccgagcg tccgccccgg cggtttcgtg aactcggcga ggtccacagg
     1201 accgcgtggc tggacgagcg catccgcggc agcctcgggg atgagcgtca cgcccatgcc
     1261 gccggcgacc atctgcacga gtgtcggcag gctcgtcccg cgaacctcca tcgattcgac
     1321 ggcgcccgat cgttcgcaca cggcgagagc ttgatcgcgc aggcagtggc cgtcttcgag
     1381 gagcaggacg gtttgatcct cgaggtcctt ttcggtgagc ttgccgcgct tgtgcacgat
     1441 cgccgcgtcg cgtggtgcgg cgagcaagaa gctctcgcgg gcgattgccg cagtcgtcac
     1501 gtcgccggga acgggaatcg ctaggagcgc agcgtcgatg aggcctgcgg cgagctggcc
     1561 gaggagccgc gctgtttgat cctcgcgcag gatcagctcg agcttcggga accgcttgcg
     1621 aaccgtcggc aagagagcag ggagccaata cggcgcgacg gtcggaatca caccgaggcg
     1681 caacggaccg acgagtggct cggcacgacg acgagcggcg tcgctcaccg cgtcgattgc
     1741 ttcgagcgcg aggcgcgaac gtccgacgat gtcctcgccg gccggcgtca cgagcaccga
     1801 gcgccgatcg cgttcgaata cctgcacgcc gaatagcttc tccacgcact gaacctgagc
     1861 ggagagtgcg ggttgcgaga tgccgagtgc cgctgccgct ttgcgaaagc tatgttgatc
     1921 cgcgacggcg acgaggcatt cgagctggcg aatcgacggc cggtccatga taggtacagt
     1981 ctatcacggc catcgacgta atcgattgga cctattaggc aggcagccgc atggtgtctg
     2041 catgctgacc attggagata cgttcccgca gttcaaagcc accgctgtga ccggcctcga
     2101 gccgggcaag cagttcacgg acgtcaccga cgcgagctac gccggcaagt ggaaggtcgt
     2161 cttcttctgg ccgaaggact tcacgttcgt ctgcccgacg gagatcgcgg cgttcggcaa
     2221 gctcgagcgc gacttccgcg accgcgaagc gcagctgctc ggcgtatcga tcgatagcga
     2281 gtacgtccac ctcgcgtggc gcaacaacca tcccgatctc aaggatctcc cgttcccgat
     2341 gatcgcggac atcaagcgcg agctgtcgag ctctctcggc gtgcttcaca aggaggacgg
     2401 cgtggcgatg cgcgcgacgt tcatcgtcga cccgaacaac gtcattcggt tcgtcgaagt
     2461 gaccgacatg aacgtcggcc gcaatccgca agagacgttg cgcgtgctcg acgctctcca
     2521 gaccgacgag ctctgcccgt gcaactggca aaagggccag aacaccctga gcgccgcgta
     2581 gggagaccac catgacgacg aacctcgacc agctcaaaga gcgacttccc gactacgcgc
     2641 gcgatctgcg gatcaacctc ggcgtgctga cctcgtcgac cgcgctgact cccgcacagg
     2701 cctggacgat cgcgctcacg agtgcagtca ggacgggtca gcgcgacctc atcgctgcga
     2761 tcgaaggcga cgggcagctc gcacctgagg cggacttccg cgcgcgagcc gccgcagcga
     2821 ttatgggcat gaacaacgtc tattaccgct tccttcactt catgggcgaa gcctcggagt
     2881 acggccacat gcccgcgcgc ctgcgcatgc aggtcatcgg caaccccgga cccaagacgg
     2941 agggagccgg atttactccg gcgaccgagg cagacaagct cgacttcgag ctcgcgtgcc
     3001 tggcggcgtc ggcgatcacc ggatgcgaga gctgcgttcg ctcgcacgag aaagcggtgc
     3061 gcgagaaagg cggcagcaaa gaaatggtgc aagacgccgt acggattgct gccgtcgtga
     3121 acgcgatcgc catcgcgctg ccgtcgtcgt aggcccgtta ccgagggcac gcgtgatgca
     3181 ccgcgccact cctagcaacc aaggagtgac cgatgaatac gcattcttcg acgggaaacg
     3241 gccatagcaa ggcaaacggt aaggcagggc gctcacatat ggatcgcacg ctgagccatg
     3301 gtgaaccgtc caagccgctg ctcaaagtcc ttccgatcaa gcggtggatc cctcaggaca
     3361 tccactcgat gatggactac gccgacggtc tcacggccgc ttccggagcc atgatggccg
     3421 acgaggaaga cgaagtcgca ttctgggcaa gcatcgcact cggcgcgtcc tcgatcggcg
     3481 tttctgcgat gaccgactac cgcctcagcg tcgccaagat catcccgatc aaggcgcacg
     3541 aggcgatcga ttacatgtgg ggcatgtcct gcatcgcagc gccgttcgcg ctgggctact
     3601 ggaagtcatc gccgcgcacc gcgctcgcgc acgtgatggt cggcgcagga acgatcctcg
     3661 cgtcgatgtt caccgactac cgctcgtaca ccgaagagcg cgcaaagcgc agagcatcgt
     3721 cgggcggcgg taacgcgggc gtgaaagtcc gccgccgttc gcaggctcag tagaggctac
     3781 gccaggcgcg cggtcttgac gacccacgcg atcgcaacaa cgccgatcac cagcgccgcc
     3841 gcaaacacga gcgagccgcc ggcctgatcg gcgatgtcgc gtgcgggctc cagtagcggt
     3901 atgcgcggtg cttcgcgcac gacggtcgcc gcaacaagcg cgctcgtccc tgccgcggtc
     3961 gcgacggcca acgctccatc gctcggtcgt cgccagatta ccgcccacgc gccgagctcg
     4021 acgacgaccg ccgcgcagag gacgccaaac cacgcgagcg ctgcaccgtc gagcgaaaac
     4081 ccagcgcgcc acagccacac cgctccgccg atcgacacga cgcgtgagcc catcgcgagc
     4141 aacgcgagtc gttgtcgccc gtcacggtcg gcggaccacg cggcgatcat cgcaaacgat
     4201 gtcgccatcg caggaaacag cacggccgac cgcggcgcga tagtgccggc gaagaagacg
     4261 cgctgaccag cggcgtagaa gtcatgccat accgggtccg ccttcatcag ctcgtgcagc
     4321 tcgctccacg accagccaac aaacacgaag cacgcaagcg atacgatcga cgcaggtcgt
     4381 cgccacttct cgctgacctt ctgaatgtag aggccataaa agccgacgat gagcacagga
     4441 acgacggcca tccatcgcgg cccgagcagc aagttcgccg tatagaagcg atgctgatac
     4501 agcagctgca cgaacagcag cggcgcaacg cccgcggtaa tcccggcgcc gagcatgaac
     4561 ggcaggcgat cgcgcaccgt cgcagcgatc ggatcgtcgc ggcgaatgag cacgaacgcc
     4621 gtccctgcga cgacgtagcc gatgaagacg gcgtgcagcg caagcgtcgc gaggtagagc
     4681 gcgagcaagc cgccgccttg cgaaaagggc caggtcatcg cgtcgcctcc gcgttcgacg
     4741 tcggcggtgg agcttgcgcc gtgccggctt cgtcgagcca cgcgttgatc tgcgtgagca
     4801 ttgcgggatc tgtcgactcg ggccatgcgc gcggcgcgtc ggcacgttcc cagcggagca
     4861 gctgcacgat cgcctcgacg tcttcggcgt taccagcgaa cggcggcatg aagcccttcg
     4921 tgcgctggag cttggcgatg ttcacgcgca tctgatcgtc ggtccatgtc cgtgtcagct
     4981 cgacgaggcc gttcgcgccg tgcagcgtgt ggcacgcgtc gcagagcacg cggtggacgc
     5041 gagcgccgcg gcggagctgt tccgtcggga agcgcgatgc gtcgcgcagc gggtatgggt
     5101 cgttggttgc ggcgcccgtg acgcggaggt gcgcgacttc atcgggcgtg atcgcgttcg
     5161 agtagaggat gccgcggacc gtgtacggtt tgcgcgcgcc ttcgcgcacg aactcgccgg
     5221 ccgccgtggc gccgaatgcg agcgcgagta ggagcgtggc cgtggcgccg ttgatgtaga
     5281 ggcgcttgta gagcaggccg acgacgacgt agccaccgat gaggagcgag gcgcctgcgg
     5341 caatcgcgac gaacatcgtc atcgccatgc tgccgcccat gagccaggag cggctgtcgg
     5401 ctgggatgac cgcgaggtac caaagtgcga tcaccggcat cagcgccatt ggtgccgtga
     5461 agcgtgcggc gcggcgaatg agcgcggcgc ggcgttctcg atcgaggtcg agcgtgttga
     5521 tgatgatgca cgcggcgagc gcggcgagcg ccatcgcgac agccgtgcgg aagatgagtg
     5581 acggccagaa cgacgggttg aagaagccgg cccagacgga gtggccgtcg gtgtagcggc
     5641 caggcgtgag ctgccagctg agaatgccgt tgatccagaa cagcgagaac caagaggccg
     5701 cggagtacag ggcgaggagg cggatgcgag cgcggtcgtt caggcggcgg ccgacgcgga
     5761 cgaaggcgta gcccgcggcg acctcgacgg agaacgcgac ccattcggtg gcccagaacc
     5821 agtggaattg gtcgaccatc aggccgatcg tgcgggcgcc gacctggatc gtcgtgaacc
     5881 acatcgccac gcccgtgagc gcgccgatca cgaacgagat caggacgagg atctggaagt
     5941 agccgtcgac gaaggcgcgg gcgtctttct ccttgccctg ttgcgcgagg cgctcgaagt
     6001 agaggagcag catgccgccg ccgatcgcga attgcgcgag gaagacgtgc aggatcccga
     6061 ccccgccgat gaccatgcct ttcatcagcg gaccgaagtc gttgaccgga tagaacggga
     6121 tcgaatccac gatcggttgg atgctatcgc gggcagcgag aacgtgcgtc gaaatgccac
     6181 cacttcgtcg cgatctggcg ctagtgtccg ctcggggtaa cgactatgcg cagtgttctg
     6241 atcgcatcat tggctgtgtt tgccgccgcg tgcggcgata aggatctcgg gactcccgac
     6301 gcgtgcaatc cgctcggcgg gcaagcgtgc atcacgccgt ggccgtcgtc ggtctacgag
     6361 agcgaggaca cgaccaccga gacgaagatg aaggtcgaca ttccggcagg cgcgctgccg
     6421 aagaacttcg acaacatcga gctgtcgccc gcgctctaca acaagcaaga cggcttctcg
     6481 tacgcggcgc cgatgatcgc ggcgttcccg accggcgtcg acccggcgaa cctcgtcgat
     6541 aaccgcaaca tcgggatgag catcacggat gcgagcccga cggtcttgat cgacatgtcg
     6601 acgggcgagc tcgtgcctca cttcgcggaa gtcgacacgc ccgccgctgc tacgccggaa
     6661 tcgcaagcgc tgtttatccg gccggcgaag ctgctcaagc cgggcacgcg ctacgccgtc
     6721 gcgatcaagt cgacgctcaa ggccaagggc ggcggcgcgc tgccgatctc cgagggcttc
     6781 caggcgatcc tcgacgacga agagacgtcg cacccgctcc tcgagaaggt gcgccctcgc
     6841 tacaaggaca tcttcgcggc gctcgaggcg aagggcatcc acaagacgga cctcgtgctc
     6901 gcgtgggact tcacgacgcg ctcgcatgcc gacgtgcaat cggacttgct gaatgcgcgc
     6961 actgcagcgc tcgcgatgat cggcgacgca tctgcactgc acttcacgat cgatacggac
     7021 acgacgcccg gtgacacgcg tatcgcgcgc cagatcaccg gcacgttcga tgcgccgctg
     7081 ttcctggata ccgaggtcac gaccgcatcg cagatcaact tgctgcgcga cggttctggc
     7141 aagccgatgg cgtcgaagct gtatcgcgtg ccgttcacgg cgatcatccc gcagtgcgcc
     7201 ctgcagggcg gcgagaccgt gccgatcatg ctctacggtc acggcctcct cggcgcatcg
     7261 aaccaagtcg cgagcggcgg cacgcgtgcg gcggctgctg aaatttgtgc ggtcgccgtc
     7321 ggtacggacc tgcgcggcat gagcgaagtc gatgttccga acgtcgcgct cgcgctcaac
     7381 gacggcaacg acggccatct gatcttcgac acgctgatcc aaggcatgat gaatcacatc
     7441 gcgctcgtgc agatcgcgaa gggaccgatg gcagcggcgg gctcgggcct gttccgcaag
     7501 aacggcgatc agccgctcat cgaccccgac aagttctact actacggcat ttcgcagggc
     7561 ggcatcatgg gcacgaccat ctgcggcatc gatcccgtga tcaagaagtg cgtcgtgcag
     7621 gtcaacggca tcaactactc gatgctgctc gagcgctcgg ccgattggcc gcagtacgcg
     7681 gtgatcctcc agggcggcta cggcaatccg ctcgtgacgg cgctcatgat caacctcatg
     7741 cagaacgaat gggatcgcac ggagcccgtg gcggtcgccg acgtcctcac cacgaccggc
     7801 ttcccgaaca cgcccgccaa gcaggtgttc atgcagttcg gcatcgccga tgaccaggtc
     7861 ccgaacgtgg gaagcgagct gcagatgcgc acgatgggca tcccgctcat cacgccgacg
     7921 ccgttccaca tctacggtgt cgaggagaag acgtccgcgc cgagcggtgc cgtgatgtac
     7981 gacttcggcc tgagcggcac gattccgctg acgaacacac cgccaccccg caacgatgtt
     8041 cactcgaaca tccgcaacaa gaaggcgacg acggacatga tgaagacctt ctacgagaca
     8101 ggcgtcatca cgaatgtgtg caccgcaccg aacggttgcg tctgcacggt cgccggcgcg
     8161 tgcggtccgg gcgtttagca ctaagctaga cccgtgagag ccgcgcttcc gttcgcgctg
     8221 gtcatcgtgg ctgcatgctt gccacccgat gcaccgccgc aggcgcctgt cgcgccgggg
     8281 cagggcaagc tcgttacgtt tccctgcgag agcgagttca cgcgcgcgga cgcgcagatc
     8341 acggaccacc tccggcgtgc gcgcctgtgg aacttcgccg agccgacgca gtccaaggtc
     8401 tcgatcgagg tcgttcagct cgcgatcgag tgcaccgacc caacgctcga ttgctacgca
     8461 gcggtcggca agagcgtgca tgccgatgca atcctcttcg cgacgatcga tcgcgcgtct
     8521 gaggatggcg cacttgtcgt aaccgtatcg gtgttcgacg tgcgtgcgaa cgcgtgggtc
     8581 cggcggtcag cgaagaaatt cccgaccgag gacgacgcgc tctacgagct caaagacatc
     8641 gtcgccgatg ccacgaaaag gggaccgtag ccgctagcgc tcgacgtgga cgtacatccg
     8701 cagtccgagt ggcagcgatt ccgacggcgt gccatcgaac tgcgcgatca cttcgaggac
     8761 gcgagtgtcg acacgcgcgc gggggtcgtc gtcgcggacg gtcttgcggc cgagctcgtt
     8821 cgtgacgcgc gaaatgcgaa ccggaaaccg gcgctcgccg aacgcatcgg cagtggcgta
     8881 tgcgggcatg ccgacggaga tggcagcgac atcggcctcg tcgagctccg cacgaatctc
     8941 gagcgtgcgc aggtcggcga cggtgatgat cgtcgatgga ttgaccgtcg tgacgagcgc
     9001 accgacttcc gcaagccggc gcaggacgac gccatcatgc ggcgcgcgca gcaccgtttg
     9061 gtcgagcgcc acttccgcgg cggcgagctc ggcctttgtg gcgtcgagcg atgcggcggc
     9121 agcggcgact tgctccgagc gcgagccctt cacgagcgcg ttgtagcggg cctgtgcggc
     9181 ggcggcctgg gcggacgaga cgcgagcggc ggcgctgtcg gcgtcgacga tgctgctcga
     9241 gatcgcacct ttctcgccga gcttctcgct gcgcgagcgc tccgatgcgg agtgatcggc
     9301 ggcggcttgc gcggcctcga gctctgcctt cgcggcgtcg agatcctcgg tgcgcgcacc
     9361 gcgcttcgac atgagatagc tcgcctgtgc accggcgacc gctgcacggg ccgacgcgac
     9421 gcgagccgct gccatgcgat cgtcgagacg cgcgacgact tgaccggctt tgacggcctg
     9481 gccttcatcg acatcgatcg acacgatgcg accgcccgtc tcgaacgcga gcgcgaccgg
     9541 atcgcggacg gtctcgaccc gacctggagc gacgaggatc gcgggccgca cgatcgtctg
     9601 gcgatccgcg gcagacgccg gttgcgtttg gtgaccgaac cagagtgcac cggcagcggc
     9661 gagaggaagc gagacgaaca ggactttacg cagcgtggac atggtgtgct ccttgatcga
     9721 tcgacgcgat atggccgtcc tcgacgcgga cgacgcggtc agcaaagcgc tcgaggcgcg
     9781 gatcgtgagt gacgacaaca acggcgcggc cgcgctgggt ggcgagctcg cgcaatagct
     9841 ccatgacgga gagcgcggtc ttggtgtcga gcgcagcggt cggctcgtcg ccgatgatga
     9901 tgggcgggtt gccgccgagc gcgcgagcga tcgcgacgcg ttgcttctgg ccaccggaca
     9961 gatcgcccgg caagtgatgc gcacgcgagc caagcccgac catttcgagt agccgggccg
    10021 cttcgctacg cgggctcttc gctttcggat ccttcatctg gatcgcgatc gcaacgttgt
    10081 cgagagccgt gagcgcggga aacaagttga agccctggaa cacgaaaccg aagttgcgtg
    10141 cgcgcaggcc ggggagctcg cgttccgaca gggccgcgac gttgcgacct tcgagccaca
    10201 cttcgcccgt cgtcggcttg agcagcaggc cgaggatcga gataagcgtc gtcttcccag
    10261 agcccgacgg gccttcgatc agcgtgactt ctcctgcgcg gatatcgagc gtcgcgtcgg
    10321 agagcgcggc cacagccgtt tcgccggcgc cgtaggtctt gcccgtgcga acgagcttag
    10381 cgagtgagtc ttgcatggtt aacctttgaa gaccgacgcg gggtcgaggc ggaggacttt
    10441 gatgatcgag agcagcgatg cgaacgagca catcgcgatg gtcacgatca gcgtcgcgac
    10501 gtacagaccg gggaacagcg cgaccgtcag gttcgctttc gccatcgcgg cctgcatgcc
    10561 gaacgcgagc acgccgccga agacgaagcc gatcgcggcc gagatcatcg cttgcgagag
    10621 gatgacgcgc acgaccatgg agttcttcgc gcccatcgcc ttcagcgttc cgtactcgcg
    10681 gatgtactgc agcgtgccgc tgtagaggat ctgaccgacg acgacgaagc cgacgatgat
    10741 gccgagcacg gccgtcgtga agaagcctgc gccgacgccg gtgcggctgg accagtacgt
    10801 gcgcgtgcgc ttgctcatct cgctcgtcgt gtacgccgcg aggtggggca gggcattgat
    10861 gcgcgccttg acgtcaatga cgttctcgcc cggtttcact ttgacgagca cgtacgtcac
    10921 ggcgttctcg ccgaggcccg gcatgaagct gcgtgcggtg cggatgtcgg cgaagacgat
    10981 gggagaggtc gtgaacgagc ggatgccggt tgtcatcgcg accacttcgg catgcacacc
    11041 ggagacttcc gtcgtgtggc cgacgtgatc gatcttgagc ttcgcgtatt cgctgcggtc
    11101 gacgacgatc gcgcccgcgt cgcgcagatg attcgcatcg cccgcgatca cgttccatgg
    11161 cgcgagcatc ggcgtgtgac cgggcgatgt ctcgatgccg acgatctgca cgccgaggtc
    11221 gccgccgttc gcgagcttga actgcgcgaa gttcatgagc acacgctcgg tgcgttcgac
    11281 gccgggcacc gacgcgactt tgtagaacgt gcgctcgtcg aacggcgacg cgaactcgaa
    11341 cgactcgctg ccttctttgc ccacccacag gtccgcgtgg ctcgagtcga tgatcgacga
    11401 cgccgtgttc atgaagccga agtagaggcc gacctgaacg agcaccagca tcacggagac
    11461 cgagacgccc gcgatcgcga ccgcgaaacg cagccgatcg tggaacagga gcttgcgagc
    11521 cagcgaccac atgctccgtc ggatgagcac gcgccgtgcc ccgattctgc gctgtagtta
    11581 cgccatgttg cgagcgcttc gacggtaacg aggcgttacc atgaccatgg agcgttaccg
    11641 cttacggcca cacttggagc gcgggcgaga tgacgccact gctcgtgact gtgagctgag
    11701 gagcgcggac ggagatgaag ccgacgccgc caccaccgcc accgccgccg ccgttcttga
    11761 gctgattcgg gctgcgcccc gcgagcgtca acgctgcgcc gcccgcgccg ccgtcgccgc
    11821 cctccgcgga gtttccggcg ccgcccgccg cggctgcagt tgtgcttgtt ccgcgaacgc
    11881 cgggatcgcc agcagtgagc acatcgccgc cctcgccgcc gccgccaccg ttcgcgacga
    11941 gtgtgccagc gatctgcagc gtgctcgctt cgatcaagat gaagccaccg gagccaccgc
    12001 cgccgccgcc gccatcggcg actttgccgc cgccgccgcc gccgccagtc gcatcgacgc
    12061 gtccgtcggt cgagatcgtg accgtctgcg acacgagcat gagcgcgccg cctcccttgc
    12121 ccgtggtgcc tgcgttcccg gatccagcgc cacctgcacc accatcgcag ccgccgatgg
    12181 gcccgggtgg acgcggttgt gcagtgccgc cgttgccgcc cgcgcgcggc gtagcgtcgt
    12241 tgttgccgtc ggcaccattg cctccgatgc ttgcaaagcc gccgccgcca ccgccgcctc
    12301 cgccaccgac gccgctcact ccgtcgatag aatcgcagtc gtcgcgatgg ctcgcacccg
    12361 cggcgccgac atcgagcaca ccgaggatgt cgacgtgatc ttcggcgacg atcgcgatcg
    12421 gcttgctacc gacgatccgt agcagcgcgc ccgaggcgag cgtgaacgag cggaacggaa
    12481 ggacgacgac tgcgccggtt gccgtcggca tttcgaccga cgcgagctgc acgctgcccg
    12541 tgccgccggg acccgaggcg gtccgcgtgt cggtgttgta gatgatgatg cccgtgagat
    12601 cgacgtcggt ctcgaatgcc agcatgcacg tcgaaaacac cgtcgacatg cccgggcagg
    12661 cgtcgagcgg cgcgtcgtcg atgatcgctg catcgtcgaa cgcatcgtcg tcgtgcgcgg
    12721 gcggctcgag accgaacacc gcacggcacc ctgtcgccag ggcgaacacg agcgcggcga
    12781 tgcgcggtgc catctgccta tcgtaactgc aacctacgcc gcagggagaa ggatcgtgaa
    12841 cgtcgagccc tcaccgacga tggagcggac ttgcagctcg ccgccgtgcg ctcgcaccac
    12901 cttgtggcag atcgccaaac caagcccggt gccgtcgggc cgcgtcgtga aaaacggctc
    12961 gaagatgcga gggagatcat ccgccttgat gccgcggccg cgatcctcga cctcgatcgc
    13021 gacgtgggtg gcgtccgcgg gcttcgcgcg aaccgtgact tgcgcgccgc gcgtgctcgc
    13081 ctctgctgca ttgccgacga ggttgacgag cacctggcgc agccgctgcg gatcgccttg
    13141 caccgccggc agctcgtcgg gtgctgcgtc gcacgaaagc gcaatgccct tctccgacag
    13201 caccggcgtc agcccacgcg ccgtcgtttc gatcagctcg ccaacatcga tcgattgcgg
    13261 cgcgagcttc aacggcttcg caaaatccag aatctcggcg accgacttat cgaggcgcgc
    13321 aagctcctcg agcgcgatgt ccaagtgctc gcgatcgtcg tcggagagct gcaacttttg
    13381 ccgcaggatc tgcacgttca acgagatgct cgtgagcggc gtacgaatgt cgtgtgcgat
    13441 cgctgcggca aactgaccga gcgccgcgag acgtcgcgca tcgtcgagct cgctgactgc
    13501 ctgcgcacgc tcgacgacga gcgcgatgcg tgctgccaaa tcgcgcgcga cgacgtaggt
    13561 atcgcgatcg agcgtgccgt gctcgatcat gaacgcaccg acgagcgtgc tatcggcgcc
    13621 gagcgccggc acgatcaagc aggcaggaag ctcggacgtt tcgtctttgc gctgcaggtc
    13681 tcccgacttc agcgtctgcg acagctccgg cgacatctgc gcagcggctt gccacgtgac
    13741 tgtcgcgccg tcgcccatcg acgtgatgcg cttgcacgcc tcggcgatcg cggcctccgg
    13801 cgccgccggc agtggctcgc cttgcacgcc aagtcgccgc gcacgccgct cgtcgagacc
    13861 cgcgaggaca ttcttctcga gccgcgggta catcgcgcgt cccactgcgg ccaacgcgag
    13921 cggaacgagc gtcgcgccga cgagcatcgc ggtctgcagc gcgctccccg catcggtcca
    13981 gcgcagcacc gcgaacacag ccgcacctcc gctgatcgcc acgatcatcg cgatcgtcgt
    14041 cgcgacaacg acctccgccg ccgccgagcg aatcgagaac agctcggtgc gcaacaccgc
    14101 cgtaccgatc acgacgaagc tcgtcagcgt cgcgaacgtc gtctccgccc acagcgcttc
    14161 ctcgaagaat ccgaggagtg atccgaacgt gtacgcgcag aagccaaagc cccagcggaa
    14221 ccacagcatc gcgatcacgg tgcgcgcgtc gcgatctttc gtgcgcttcc acgcacgcgc
    14281 gccaatgaag aagacaacga gcgtggccgg cagatagaac gtgaactcgc tgacgcgcag
    14341 cacgttccac ggcttccccg cgtcgacgag cgcggtcacg agtgcacccg aaatcgccca
    14401 cgcgatgagc agcagtcgcc agcgccactt catcggcgcg cgccgaggaa agctatggag
    14461 gaagtccatc gtgatgacgg ccatcgccat cgtgccgagg ccacagggca gcgtgaccga
    14521 ctcgctcgtg atcgagccgc cggtcagcac cgtgatagcg cgccacaaga tcatgccggc
    14581 gtcggtcatc gcgaggagac cgaacatcac gttgtcgcgc ctcttcggtg cagcgcgaat
    14641 gacgatgccg ccgtacgccg cgccaaccgc cgcgatgagg atcagcagca agccagcgat
    14701 caacacgcgc ccaccgccag gacgatcgcc gccgccgccg ccgcgacgcg ttggtgcacg
    14761 tggtcgctgt gtgggatcat gactcgtcac ctcccgtcgg tttgacgagg ccgtactcct
    14821 cgatcttgcg atcgagcgtg ggccggctga tgtcgaggac ggcgcacgcg cgtcgcttgt
    14881 tccactcggt gtgcgcgagc acgcgcgcga tgtgcctccg ctcgatctcg cgcaaggtca
    14941 tgagcgagcc ctccgcatcg acgtccgctg caagcggctg agctgacgag ccgagcggca
    15001 acagcgtctc gtcgagcaca tcggattttg cgagcacgac cgcgcgtgtg agcgtgttct
    15061 cgagctcgcg aacattgccg ggccacgcat atgccgacag ctttgcgagc gccgcgggcg
    15121 tgacgtagcg gacttctttg ccgaggtcct tgttgatctt ggcgagcaat ccctcgacga
    15181 ggatcgggat atcgctcgcg cgttcacgca acgaaggcag cgcgatctcg acgacgcgaa
    15241 gccggtagta caggtcctcg cgaaacgtgc cctttgcgac catttgcgac agatcgcgat
    15301 gcgtcgcggc gatcacgcgc gcttcgtgct gcatcgtctt cgcgtcgccg acgcgctcgt
    15361 acgtgcgctc ctgcaacacg cgcaacagct tcgcctgcag ctcgagcgga atctctgcga
    15421 tctcgtcgag gaagatcgtg cccttgcccg cgagctcgaa ccgtccaggc cgatcggcca
    15481 cggcgccggt gaacgcgccc ttcacgtggc caaacagctc gctctcgagc acgcctggcg
    15541 ccaatgccgt gcagttcacc gcgacaaacg gcctgccttc tcgcccagct tccgacgcca
    15601 tgtggatccc gcgcgccacg agctccttgc ccgttcctga ctcaccgcga atcaacaccg
    15661 gcgcacgcgt cgtcgagacc gcaccgattt gcttccagac atcgcgaatc gcggcgctct
    15721 tgccgaggat gtcgccggct tcaggtccac gcgcggtcag ctcgacgagt tgctctcgcg
    15781 cctcgcgact atcgagggcc gtcttcacga ccgtgcgcag ccgatcgatg tcgaccggct
    15841 tcacgaggta ctcgtacgcg ccgagctgga ttgccttgac cgtcgactgc atgtcgtcgc
    15901 gcgcggtcac gaccacgatc gtcggtgcat catcgcgacc cgcgatcgca ctcagcacat
    15961 cgaagcccga gccgcccggc agcccgagat cgagcagcat tagatcgaac ggaccgcgtt
    16021 gcccggcgac gggctccccg cgttgcaccg ccgcgatcgc gccgggtgcg tcctgcgccg
    16081 tcgcgacctc atagccctcg cccgcgagaa acttctcgag cgttcggcgg atcgagcggt
    16141 cgtcgtcgac gatgaggatg cgagccacgg caggtaggtc cagtccacgg gccatgccat
    16201 cgcaactcag cgaaaccgcg gagacgcccg tggatcgaga gtaacgaaac gttgctcccg
    16261 atggcattta tacgatcggt atttgacagt gaatgaaaca gtgttacata caaggcgtgc
    16321 agacggactc cctcgaagag ctctggccgc ttggcgacct agtcgcggaa gcggcgaacc
    16381 ggctcgaggc gttgccagcg ccgaagaacg ggcaggttcg cgcggtgccc gacgagcgca
    16441 ccgttcgcta ttacgcggcg cagggcctgc tcgatcgccc ggcagcgatg cgcgggcgta
    16501 cggcgctgta caaccggcgg cacctcgcac agatcgtcgc gatcaagcgg atgcagcaag
    16561 cggggcacag cctcgccgac attcagaaga tgtgggcgac cgtcgaggac tcgacgctca
    16621 cgcgcatcag cggcgtcttg atgacgagca cggtgaatcc tgcgggaggc ccgatgcgca
    16681 cgaacccgaa gtcgccgcgc aaggacttct ggaagcagct gccggagatt ctgccgacgc
    16741 cgaccgtcgt gacgccgcta ccgcggactc gtgggacgac gccactacca ccgacgcctg
    16801 ggacgacgcc actaccgccg ctgacgcggg ggactcaagc ggcggagctg cgcgttgaag
    16861 tagcgcccgg cgttgccgtg gttgtcgcgc tcccgacgga gcgcagcatt tcgatttcac
    16921 ccgccgacgt gcacgcagtt cgcgtagccg cggccgctgt cgttaccgag ctcgcaaatc
    16981 gcgggctcct tgcccggggc acgggcacac tggaggaaga gccatgatcg cagtcgtgtt
    17041 gatgtctgag gaggaggtgg cggcgcttgg ccggaaccgc gacgagaacg cgggattcgg
    17101 ctgcttgcgc acgccgcagg ggtgcgtgcc gatgagcgcg atggacgtcg acgcgcgcgt
    17161 cgtcggcgtg atggctgcgg tcgagctcgc gcagacctac gtgaacacga cagcgacgcc
    17221 gatcgaggcg acgtacatct ttccgatgcc ggatcgcatg gcggtccatc gatttcggat
    17281 ggaagtggcg gggcgagtga tcgagggcgt ggtggacgag cgcggcgccg cgcgcgagca
    17341 ctacgagcag gcgatcgcgg caggtcatcg cgccgcgatt accgaggagg aacgcgctgg
    17401 ggtgttcacg ctgcgggtcg gcaacttgat gccgggcgag gtcgcaacga ttcgcttgag
    17461 cttggtggga ccgctgccga tcgatgacgg cgaagtgacg tttcggtttc cgctcgtcgt
    17521 cgcaccgagg tacatgccgg gcgcgtggct cggcggcgaa caagcggggc tcggtgtggc
    17581 gggagatacg agcttggtgc cggatgcgtc gcggatttcg ccgcccgtgc ggctgccgca
    17641 cattccaaat ccagtgcggc tcggaattcg tgtggcgctc gcggacggtc acgtgcgtga
    17701 tgtcgcgtcg agcttgcatg ccgtgacgtc ggcgatgcgc gacgcgcagg tgatcgagct
    17761 cgggccgtcg gagaggctcg atcgcgactt cgtgctgcgg tggcgcctcg acggtgacga
    17821 gctgcgcagt ggactggttt gtgcggacga cgcggacggg aagggcggca ccttcatgct
    17881 gacgttcgtg cctccgagca cgctcgcgat gtcgcagaag ccgcgtgatg tcgtgttcgt
    17941 gatcgatcgc tcgggaagca tggaaggctg gaagatgcag gccgcgcgtc gtgcgacggc
    18001 gcgcatgatc gacacgctga cttcgcgcga tcgcttcttc gtgatcgcgt tcgacaatgc
    18061 gatcgaagtg ctgcccgacg tgcagctcgg ttacgcgacc gatcgcatgc gcttcaaggc
    18121 ggtcgaggag ctcgcgaagg tgaacgcgcg tggcggcacg gagctcgagc agccgctgtc
    18181 gctcgccgtg cgcacgctcg cgggcggcca cgatgatcga gagcgtgtca tcgtgctcgt
    18241 caccgacggc caggtcggca acgaggatca catcctgcgg caagtcgcgc ccaatctgcg
    18301 caacgtccgc gtgttcacgc tcggcatcga tcaagcggtc aacggtgcat tcttgcgccg
    18361 tctcgcgggc gcaggcggtg gcctgtgcga gctggtcgag tcggaagatc gcctcgacgt
    18421 cgtgatgagc aaagtccatc gtcgcattgg tacgcccgtc gcgacggaga tgcggctcga
    18481 tgcgcacggg atggacatcg tgtggaatca ggtcgcgccg gccaagttgc ccgacgtgta
    18541 cgcgggtgcg cccgtcgtcg tgctcgggcg ctatcgcggt gcggcagcgc tcgatgcggc
    18601 ggtcaccgtc gacggtacga gctttggcga gccgatgcgg cttcgcattg cgcgcgagtc
    18661 gacgactgga accagcgcgc tcggtgcgag ctgggcgcgt gcgtacattc gcgatctcga
    18721 ggacaagtac gcgacgggtt cgcgcgagct cgaaggcgaa atcgttcgcg tgtcgaagca
    18781 gtggaacgtg ctgtcgaagt tcaccgcgtt cctcgcagtg gatcgcagtg aagtcgtgaa
    18841 tccaggcgga cggctcgcgc aagcggtgca gcctgtcgaa tcgcccgcgg gttgggaagg
    18901 tgctgcgtcg ggagccgtcg gcgggatgcc gagcctgccg ggaggcatgc cgagcccaat
    18961 gagcggtgga ggttcgttgc gcagtcgcgc ggtgatgccg gctcgcgcga tggctgcggg
    19021 tcgccccatg gcagcaggcg gcaagatggc gcctccgatg gacggagcgt tcgcctcgcc
    19081 gccaccaccg ccgatggcgc cgcgtccgac ggagtcggct ccgatgcccg ccttcgccga
    19141 cttgtcgatc gatccggcga gcccgagtcc cgcggagaag tcgatcgccg cgccgcggcc
    19201 ggctccgcgc acaccgggac cggcgagcaa cagcatgtcg gcgccgccct cgtatcggcc
    19261 gccgatgcag caagcaccgg cgaagggaaa gcgcgaggcg gaaccgagcg acgcgcttgg
    19321 acgttcggcg tcgggcgatg cgtctgcgta tctcgcgaag ctgcaatcgt tcgcgcgcga
    19381 gctcgaagcg gaggcgcgtg gtgcggtcag cgtcggcaag ctgaaattgc tgcggcagcg
    19441 tctccgcgaa tgggtcgaag atctgcgttc ggtcggtggc ttcgactcgc ttgccgacgt
    19501 cgtcgaacag ctcgcgcaca agctcggcga cgcgatcgcc gcggccgatg tggcgacgca
    19561 ggtggcgtcg ctcgcgaccc agctcgcgaa tgttgccgcc ggtggcgctg tgccaccacg
    19621 atcgacgggc aggcccgcgt tctggaagta acgcgctgac ggtgctacga ccggaacgtg
    19681 aagctcgcgg tctttgttgt ggtgctcgct ggttgttcgc gaccagcgac gccgcgccat
    19741 gacgagacga agcttgcgcc gttggatgta gcgccggccg tcgtcctgga gccgatcgat
    19801 gcgcgcacgg ccgacgctac tgccgatgcc gcgagcgttt cgatcgtcga tgcgcccatc
    19861 aagtggagcg acgagcgcga gcggctgacg ctggagtacc ggcgacgtca cagcgacccc
    19921 gatgcgagcg acctcacgat cgcgccgaag gtgatcgtcc tgcattacac ggcgggcggc
    19981 tcggcaaagg cgacacgcgg ttacttcgac aacacgaaga tcgaagcggc gcgcaaggac
    20041 ctcgcacgcg gcggcgcggt gaacgtgtcc gcgcattttc tcgtcgaccg cgatggcacg
    20101 atctacaggc tgcagcccga gacgcgattc gcgcgtcact gcatcgggct caaccacatc
    20161 gcgatcggca tcgagaacgt cggcgacgag gcgaagtatc cgctcaccga tgcgcaggtc
    20221 gaagccgatg ccgcgctcgt gcgcgacctc accgcgcgat ttccgatcac gcatctgctc
    20281 ggtcactacg aggtgatgaa gttcaagcag cacgcatatt tcatcgagcg cgaggcgggc
    20341 tatgcgaact cgaagccgga tccgggcgag aagttcatgc agcaggtgcg tgaacgcgtc
    20401 ggcgatctaa agctgtcggg cttgtgagcg acgcgaacta gcgccgcccg ccttttttca
    20461 ggacgacgtg atgcgcttgg ccgttctgct tcggcgtcac tcgctgcgtg tcagtcgcga
    20521 agcccggctt gctgagcgtg atggcggacg attcgaaggc cgcaactttc acggtcgtcg
    20581 gcgtcacgcc cgcggacttg ccaccgatcg tgatcgtcgc gcccgacgga ttggacgtca
    20641 ccttgagaga gaacatcgtc ttgccgagct tgatcgtgat cttgttcgcc ttcaccgcgg
    20701 acggcgtgaa cgtgcggtcc gtcgcgagga agcgcgcctt tttcagcgtg agcgttgcag
    20761 cggttccgca cggcagctcg agcgagaacg gcgtcgtgcc gacttttgct tttgcgagat
    20821 agatatcagc gcccgtcgga acgctcgaga cgtcgacctt gcacgggcca tcgtgtggcg
    20881 gcggcggcgc aaccgcgctg ccttgctccg gcgtttcgat cgcgacttgc gagcccgcgc
    20941 ccgaccccgc gtccgagcct tcgtcgttcg cggacccctc atcctcgacg accgcagcgt
    21001 cggggacgcc catcgtgatc gctcggtcgg cgctgccttg cgagccgacg cctttggtga
    21061 tcagcgccgc ggagcctttg ccggatgcgc ctggcttctt cttcgagcca ccggcgatga
    21121 ggagcacgag caaaaatcca gcgaccacgg cgaccgccgc agcggcgatc acgatgatca
    21181 tgcgcttgcg cgcggtcgca gcggacggct cgggcgaagc gccactgcca ctcgccccga
    21241 ggtcgtgcat gctgcccgga gcggcgggga atccactcga cgttccaaag cccgagaagc
    21301 cgccgcctgg aatcggcatg tttcccgtcg gcgacatcat cgccgcggcc ggtaggttcc
    21361 ccgttccgac gggcggcatg ggcgcgagcg gcggcggaat catttcggcg cgaacaggtc
    21421 ccgtctgcct cgagggcggc ggcgcgagcg cggcaggcgg cggcgcgacc ggatcgttcg
    21481 gatcgtcgtc gtcaagagcc ggcatcggtt tgagcggctc catgagcgtc ggatcgatct
    21541 cgatcttcgc ttcaccgatg ccggtcggtt tctcttccca ctcgacagcc tcgagcgctt
    21601 tgctgctcgc gacggacgtc actgggttgc cgctccgtgg cgtctgccgc ggcgggggag
    21661 cgggcttctc gacgaccggc ggcggcgcgg cggtcgcggg aagcttctgc ggctccgacc
    21721 agccctcttc gggatcgagc gagatggtcc agttgcccgt cggcggtccc ttcggcgcct
    21781 tcttcgcttc gacctggact ggaccgctgc cggcgtcacc ggcttctccg tcttcttctt
    21841 tcgacggcag gaccgtcgga cccgacgcgt cgggcgtcat cgtccagtct tccgtcggaa
    21901 tcgcggccat gatctccgac gcgcgcatgc cgccggagcg cgacgagacg agaccgccga
    21961 gatccgtctt cgcgaatgga tcgacttgcg gcatcggctg ctcgtcggga agcgccgcgg
    22021 gcttcggggc gggcggaacg gcgctgaggt ctgtcttctc ttcctcgtcc gcgaccatcg
    22081 gcttcgccgg cgggcgaacg agcggcagcg gcgcggcagg cgtcggatga cgcggcgtga
    22141 gcggtggtgt cgtcggattc ttgccgcgcg gacccggcat gccctggcgc ggcttcgcga
    22201 cgatcggcgg cgctggctcg ccttcgcctc gcggcgcgtt cgacggcggc ggcacgacac
    22261 cgacgggctg cgtctcgaac ggcgtgcgga tcgcgggaaa tccgatcgac gtcgctttgt
    22321 gcgccttcga tgcgcgcggt ggctcgggga gaaggctcgg cgcggcctct gtgctcggag
    22381 gcggcgacgc ggccggcatc ggatcggtgc tcttcttcgc cggcatctca ccgatggacg
    22441 tcgattcgtc atcgctcacc acgggcttgg cgacgggcgc ggacttcgga gcgccaacac
    22501 catgctgcgt ttgcagcagc ggattctccg gcggtgcttg cgctggaatt tctttcgtcg
    22561 ggttcggcgg cgcttcagtg cccagcatcg tgttgagcag cgcgttggcg ggcttcaccg
    22621 gtgcgggcgc tttgtcgatc gcgggaaacc cgatcgacgt catcttgagg ctggggtcgc
    22681 gattggccgt cggcttcgat ggctcacgcg cgatggcagc gaccggcccg ggcttcgatg
    22741 gctcacgcgc gatcggcgcg attggcgcgg cgctcggctt cgaggcttcc ttcgcgatcg
    22801 gcgtcggctt gggcagctct cgcgcaggtg gttgcggcgg cgcggtgtta acggtgggct
    22861 cgccgggaat cgacggcgtc ttcgcgcggc ccggcggtgc cgacggaatc gagggcacga
    22921 tgaactttgc tttgccgctg ccttcgccgc cgatggcgcg cggcgcgttt gcggtctcct
    22981 tcagcgtcga tggatcgcca atgacgacgc attcggccag cgcgttcgtc gcatcgatgc
    23041 gaccgccggg agcgggtggc gtggggcgag gctcgggcgg cacgttcgcg ggacgcggcg
    23101 gcacactcgg cgccgggacc ttcatcgcga ggcgggcctt ctcgagctcg ccgatgatga
    23161 ctttgctcgg ctcgtccggc tcgacgaact tgatcgtcat gccaggtcgg ccatagagca
    23221 cggtcggctt gttggacgat tggaggatct ccgcctcgcc ctcgatcatc acgccgccat
    23281 ttttgagcgt gactgcaatc cgaccttttc gcccagccgg gagcggtgcc tgcgacggca
    23341 cgaagagtcc ggtgcgatca gcgtaacgcc tgaacgcagc aacgaactcc tcggcagtac
    23401 cgcatgccga gaccaggtag aggggggccc ccaacgtcac cttccggaag tgtaggcgaa
    23461 aagcgagccc accgaaacgc acgacgggca cccctggggg cgcccgccat acaagattcc
    23521 cggtgtggga ctcagaagat catgaagtcg tgcgcgcgga cggtcttgcg gccgttctcc
    23581 gacgcgcggc gggctgcctg atggacgagc cagccgacgt agccgttcag accctcgagt
    23641 gcatcaccag cggtgttgca gccggcgtcc ttgatcatct gcttcacctt cgacgagacg
    23701 agcagcatct ccttcggacc accgccgccg ccagacttac cctttttcgc tttggccatt
    23761 tctcttctcc tgcttgattg aaagcgcacc tcttttacac cggctcgacg cagagaaaag
    23821 ggtgaacgcc ctaaaaagcg ctctacgtgc gatttggtgc ggatctaccg atcgagcttg
    23881 acgacgaaca catccgactt gggcgctttc gacagcactg gatctttcgc gtcgatgatc
    23941 tggtcgatgg ccggctccat cgccgacagc ttgaagcgat agaggccgct gatatacgcc
    24001 gcgcccttat cgtcgacggc gaggacgcga gcctgatcgt ggtccttgtc gccgaacgtc
    24061 tgaatccaga cgagctcgcc ctttgcgttg agcttctcga cgaagacgtc cttgttcggc
    24121 gttttcgtcg tcacggcgcc tttgccgaaa tcgacgctgc cttcgaacca gcccgacacg
    24181 acgacgttgc cagccgcatc cgccgcgacg cccgaggcga cgtcgacttt cgagccgccc
    24241 atcgtcttgg cccactcgag cttgccggcg ccgtcgaagc gcgcgacgaa ggcgtctgct
    24301 tcgccgagcg attggtgatc gtcgccttgg ccgaacgaga tgccgccttt gttctcgaac
    24361 gacccgacga tcgtcacgtg acccgacgga tcgacggcta tgccgttggc cgcttcatcg
    24421 aatgcgttgc cgaagcgctg cgaccactgg tgatcgccgt tggtgtcgta cttcgcgagg
    24481 acgacatcgt cgtcggagcc gccacctgcc ttgagctccg cgccgcccca gttcgcgaca
    24541 tctttgaaga tgccctggat gaagatgttg ccgttggcgt cgacgccgat gtgacgaatc
    24601 gaatcgttgt agcgaccgcc gaacgtcttc acccactcgg tgttgccgtc ggccgcgagc
    24661 ttgatgagca gcgcatccgt gccgcccttc gacttcaccg cgacgtgctt cgcaccgacg
    24721 gagatgtcga gaccatcggt gaacgagccg ccgatgaccc agccgccgtc gggcatcgct
    24781 gcgatcgcat tgacgccatc ggaatcgatg ccgcccgcgg tccacatcca ctcggcgttg
    24841 ccgtctttat cgaatgcggc gatgtagagg tcgtcggagc cggccgactt cttcgtctgt
    24901 cccgcgagcg tgatctcgtc gaggaatgac cccgcgacga cgactttgtc gcctctcgtc
    24961 gcgactgcgt tgccgacgtc ttcgcgcgct gcgccgaacg tctgcgccca cgcgagcttg
    25021 ccatccgcgc ctaccttcgc gaggaacgca tcgctcttgc ccgcggactt gcgcttgccg
    25081 agcttcgggc cttcgccgcc gaaatccgtt tcgccctcga agtagcccgt cacgaatgcc
    25141 tcgccagccg aattcactgc gatgcccttc ggtacgtcgg taccgaggcc gccaaacgcc
    25201 gcttgccaga ccgccttgcc cgtcgcgccg cccggatccg ccgcgagcgc gggcagctcc
    25261 ttcgtcggca cctgcttctt cggcgcgacg ttcaccgtcg agttctcgac ggccttggtc
    25321 tcctctttct tcggcgcggt cttgccgcag gcgacgaggg acgcagtgac gagggcgagc
    25381 gtagtcggac gcatggcgag acgatgtcac gcacgccgtg cgaggtcgag taggatgcgc
    25441 gcgtgttgcg cttcctgatg atcgtgggaa ccgtgctcct cgccggttgt ccaagccata
    25501 cgccggtggc gccgaagtcg cccgacgaca cggcacttcg cattcgggtc gcgcaagccg
    25561 aggtgaaacg cgccgacggt gtcgcggagc tgctcgcgct tgccggcgac aaggatgtcc
    25621 acgcgcgcga gctcgcactt cgcggtctcg gtcgcagcgg agatgcgaag gcctacgccg
    25681 cgctcgagct ggcgctgcgc gatccagagc cgcgcgtgat cgctgcggcc gctgcggcaa
    25741 tcggcgtggc ggcatcgctc gacgacaagc ctctgtcgtc gtcgtagctc gtcaccgcgc
    25801 tctcgacggc gaaggatccg cgcacgcgtg ccgcgatcat cgaagcgatc ggccgcgccg
    25861 gcgacgaagc cgcgcaagac tcgctcgtcg cgctcccaga gctcgagcat atcgcgctcg
    25921 cgtttggccg gttcggtcgt cgcaagatca agctgcacga cgcagctcgc gccttcctcg
    25981 tcgctgcgag cagtcacgcc gacccgcgcg ttcgctacgc cgcgacgtac gcgctcagcc
    26041 gcgaattcga gccacctgct aatgaggcag cgaccgcagc actcgtcgcg cgcattagcg
    26101 ataacgaagc ggaaattcgc gcacaggcga tcgcagcgct cgcgcgacgc aagtcagtcg
    26161 cttcggcgca aacgcagatc gagagcgcgc ggcaggatgg cgattggcgc gtgaaggtcg
    26221 aagcagcgcg agcgctcggc gaaacggatg atcgcgccat catcgctcca gctggttcgc
    26281 cctggcagct gtgtgtagcg gccgcaaagc cctcgccggc gactgcgacc acggtcgatg
    26341 caatcgtgcg ctgtccgctc cccgatcatc tcaagcttcc gctgctcgcc gatctcttcg
    26401 acgagaagcg cagcgacgcc acgttccggc gcgcggcgct gcggcagctc ctcgcaagcg
    26461 atgatccgcg cgtgcgcggc gcggggctca cggcattgcc gaagacgtgg aaggacggcg
    26521 acgaagcagc ccaggcgacg atcgtcggca ccgtggtgag cgccttggcc gcgaagaatc
    26581 aaatcgtcgc cggttcagca gtcgatgcag cggagcagct gttcgacgcg atgggcgact
    26641 cgcatcggct tcgcgccacg ctcgaggcgg cgctcgtgca gcgcgcgacg acggagtccg
    26701 atgcggagct cgtgcagtcg atgtacgcgc tgatcggcaa gaagaagatc gggaccggcg
    26761 cggaggcgtg tcgcatgggc ctcgacttgc cggcggcagc cgcgaaggca gcgagcgaat
    26821 gtttgaccgc actcggtgaa ccgaaagacg cgacgccgcg catcgtgcct ccaccacccg
    26881 tcgatgtcgc gagcgtgatc ggaaagcgcg ttatctggca tgtgacgacg acgcgcggtg
    26941 ggatcgatat cgagctccgt cccgacgtag caccgtgggc ggtcgccgtg atcgtctcgc
    27001 tcacgcagcg cggctactac gacaagaaag agtttcatcg cgtcgtcccg aacttcgtcg
    27061 tgcagggcgg cgacccgacg gagtccggct ggggagggcc tggcttcacg ctgcccgcgg
    27121 agcccgcgag tagcctcgac ggaccgggct atgtggaggg cggtgtaggc atggcggacg
    27181 ccgggaggga caccgcggga tcgcagtggt tcatcatgca ttcgcgtgcg ccgcacctcg
    27241 acgcgcggta cacgtgggtc gggagcgtgc tcagcgggca gaaggtcgcg aatgcattgc
    27301 aaatcggcga tcgcgtggaa tcggcaaccg tggagctaac tccgttgtct cgctaggcat
    27361 gagaaataga cctcggcgcc gtgtcgttcg ttccagacat cacatgcgcc gatccacccg
    27421 gaaggtcgtt ctcgccattg gaatcgccag cctgaccgtc agcgccaccg ccgctgcaaa
    27481 gcggatgatg agcgacttcg aattcggggg cagcacggtc gcgttccaga tgcaggggcg
    27541 cccgatcggc ggcagcaagg cagcgtcgcc gacggcggcg tatctcgcgg gctcgcgcat
    27601 cgccgcgatc gacgacggcg cgctcgtgat cgacgcggac tcgggtcagc tcattcgcac
    27661 ggacaaggcg ggcaagcagc tcgcgtcgct cgcgatcggc aagaacgctg gccttctcac
    27721 gtacgacccg gtcggcaagc tggcgtacgt tgcggaccgc gtcggcaatc gtgtcgtcag
    27781 cgtgaaggtc ggcgacaaga aattggaagt cgcgaaagag atcaagacgc cggccgagcc
    27841 gtacggcatc gcgctgtcgc ccgatcgctc gacggtgatg gtcacgacga tcgccgatcg
    27901 cacgctcgtt gcgttcgacg cggccggcaa agagaagtgg cgcacgccgc tatcgcgcga
    27961 gccgcgcaac atcgcggtgt cgcccgacgg cacgcgcgcg ctcgtcacgt atctgacgac
    28021 gggcacggtc gatcaagtcg acctcctcga gacgcatcgc accgagcaca tcgcactctc
    28081 gacgcagcag ggtacgcgtc gcctgagccg cttcaatcag cagcagccgg acagcttcgc
    28141 gcgtggctcg ttcaccgcgc tgttcatggg cgagcaccaa gccatcgttc cgttccaacg
    28201 cgagacgccg gtccaacaga tcggcggagg cgagcgcacg ggtagctacg gcggcggctt
    28261 cgatccgccg atcacgcacc agctggcgtt cctggcgacg aacggcgagc gcaccgcgca
    28321 gatcacggcg cagatcggcc agcatcagcc gcgcgcactc gcgtgggatt cggcgcacga
    28381 cgcgctctac gtcgcgggcc tcggcagcga ctcgatcatg cagatcaaga acgcttcgca
    28441 aggcacgatc gcacagggca ccgaggtcaa cctcgcgcag aaagaacgct gcggtcccga
    28501 cggcatcgca atcggcgctg acggcaacct catggtgtgg tgcgcattca cgcgcagcat
    28561 caagcgcgtc gagatcgtcg acgccaaggg cgagctgatg gcgatggcga agacgacgga
    28621 cggccccgag ctcgtcgcgt cggcgatgtc ggagaaagaa catcagggct acgtcctgtt
    28681 ccacgccgcg cagccggcgg tctccgcgcg cggagcgatg gcatgcgcga gctgtcaccc
    28741 ggacaaccgc acggacggcc tgtcgtggcg catcgacaag caagagctgc agacgccgct
    28801 cctcgcgggt cgcatcgtcg gcacgcaccc atataagtgg gacggcggcg acaaggatct
    28861 gactgcgagc ctcacgtcga cgatgaagcg cctcggtggc cgcggcctcg acaaggtgca
    28921 gaccgaagcg ctcgccgcgt atctcgagca gctaccgaaa ccgatcgcac cgtcgcgcga
    28981 ttcgacgcag gtcgctcgtg gcgagaagct cttcgactcc gccgagctcg gttgccggag
    29041 ctgccacgac ggcaaggcgt tcaccgatca ggacgtgcac aagttcacga gcgtgacgct
    29101 gcccgacgcg gacacgccga gcctcctcgg tctcgcggcg agcgcgccgt actatcacga
    29161 cggcagcgcc gcaacgctcg agtcgctgct tcgcgaccgc gctcgcgtgc acggcatggc
    29221 cgagaccgca aagctcaacg atacgcaggt cgccgacctc gtcgcgttcc tcgagtcgct
    29281 gtaagcggcg ttataatggg cgccggtgtg gcgtctgctc gtcgtgctcg tcgtggccgt
    29341 cgtgagctcg ctcgtcgggt gcggtcgcgt cagcttcgac gcactcgcgc tcgatagcgc
    29401 gatcgatgca cgtcctgacg cacccatcga cgcggcgttt ggaccaccgc agctagtcgc
    29461 cgtgtcctcg tcggtcgacg aggaagatcc gtcgctgacc gacgacatgc tcgagatgta
    29521 tttcgacgcc aacccgggcc tggtgcgggt cacgcgcacc gccatcgatc agccgtggtc
    29581 gagccgtgta ctggtgatgg agctcggcac gggcagcacc gtgaataacg cgaagatctc
    29641 gggagacgga ctcgcgcttt acttcacgag caggcgcgtt ccgtcggatg cggccgacat
    29701 ctggtatgcg actcgtccgg atcgcacatc gctgttctct gcgccgcagc tggtaggcgg
    29761 cgtccagacc agcagcgacg agtacgagcc gatggcggtc ggcgacggac acgtcatcta
    29821 cttttctcgc gccatcctga ccgagtcgct gtggcgtgcg agccgtgcgt caacgatgga
    29881 tcccttcgcg gataccacga acatcccgga gctcgactca cccgcctacg aaggcgggat
    29941 ctgggtcagc gccgacgagc gcgttgcgta ctttcacagc gatcgcgtga gctcggcgga
    30001 gcgaatgatt tttcgcgcat cgcgcgcaga cgctagcgat ccgttcggta cgcccgttgc
    30061 tgtcagcgag atcgacttgc ccggcaaact cgaagacccg tggctatcgc ccgatggtca
    30121 cacgctgtac ttcgcatcgg acacgagcgg ccagtacgac atctacatgg cgacgcgcta
    30181 gcggccggcg ggctcgcgac gctcgacggt ccccgcgagc tcgacacggt catcggcgac
    30241 ggcgacgtct gtcaccacca catcgccgcg cctcgcgcgc tcgaaggcct tgttcgcctc
    30301 cgtgcgattg cgcttgagca atcggagcgg cacgcgcccc gcatcgctgc agccgaacat
    30361 ctcgtgcttg cccttcgcgg aaaacgccga gctgacgacg cgccacgcgc ccggcgcgtc
    30421 gacgagcggc gcggcatcgt ggcggagcac gaggtagctg aatttcaggc cgctgtcgcg
    30481 taggtgcgtc aggcgcgcga gctctgctgt gcgcggtgga agctggacga ggcgatcttc
    30541 gtggcaccag tcgtcggcgt tcgcgagcat cgggcagggc gcgagcttgc gcgtgcacgg
    30601 tgcgaacacg ttcgccttgc cgatgccgat gatcgcgtcg cggagctcgt ggagcgcgcg
    30661 cgacgtgtcg cggagtgcgg gctcgatgat gatgaccgca ccgtcggcgg cggtcgcagc
    30721 aagcgcgccc tcgacgagct tcacgcgtgc gggcatcgca agctcgttga ggacggtgcc
    30781 catcacgacg aggtcgccgt gtggcagcga cgttgtctct acatcggcca cgcgggtcgt
    30841 cacgccggcg atcacgccgc gcttggttgc tagctcgcgc agtgcagcgg cggcgatgcg
    30901 cagagcgtcg acatcgcgat cgatcagcgt catctcgaat gccgcctcgc cggttgcgtc
    30961 ggttgcgagc atggcgacga tgccgaggct catcgcgcca catcccgcgc caacgtcgac
    31021 gatgcggagc ggccgtcgcg cgggcagcgc gttgcgacca gcgagctcgc ccagcgggat
    31081 cgcgatcttc atcgcgtcag cgaccgtgaa gaaggcggcg cgtgccgcga ggtctgcagt
    31141 cttgtcggca ggcttcgcga gatggccgcg gtcactcgtg tagcgcttgc tacgatcgac
    31201 gatcgcgcgc gtcaacgcag cggtctgcag cggttcgtcg ccgatcacgt cgaccgtcgc
    31261 ggcatacacc gcgtcttcga gctcgacggg aacgacccac gtcatttcga aatgtctagc
    31321 gtgacatcgg cgagcccgcc gtccgtaacg acgacctgcg cgtgcgcgat cttcgcaggc
    31381 tgacccgcgc gcggcggcaa ccacgcggtc gccgtatacg tgccggctgg cacatcgcgc
    31441 aacagcactt ggccgctcgc ctcggtgatc gcgacaaacg gcgaatcggc gacgacgacc
    31501 cacgcgtcct cggcatcgcg gctcttgccg tcctcgccga cgatctcgac gtgataggcg
    31561 gccttcgcgt ccacgccgac ctcgatgctg tgacccgcaa tgggcaaata cgccgtcagc
    31621 gcacccgcgc ttgtcgcagt tcctgcgctg tcgctaaatg cggcgcctgc tttcgtgatg
    31681 acgaccttcg caggcgattc cgcagcactc gcgacgatga gcttgtcgct tgcgagggcg
    31741 acacgcggcg tgagcgcgca gtgatcgagg acgacgcgcg cttcgcgagt cattgcggcc
    31801 gcagtcatca ccggggcggt cgtcgcggag cctgtcgcgt tcgatccgcg cgacttcgac
    31861 agcgccgcgg cggtcgtcgc gggcggcgca tcgatcatca cgaacacgtc ggggatgccc
    31921 cacgtggtcg tcggtgcgac cgacgcggcg cgcggggtgt tgcacggcgt gcggcccgac
    31981 gaggcccgcg cggcgagcgg cacgtccttc cactcgacgc ggagctgcac gtcgcctttg
    32041 ccggacggtg gcggcgcggt cggatcggtg acgggggcgg agtcttgcgg atgctttcgc
    32101 gcgtcctcga cgcctttcgt cttgcccgag cacgcgcaga gcagcgcgag cacgatcaga
    32161 acattcgatg aacgaacgtt cattcactca gctccacgtg cgcagctccg agacgatgac
    32221 gtcgcggtcg gcgcagagat cgttgacg
//
LOCUS       DATLBZ010000091        12693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3018759, whole genome shotgun sequence.
ACCESSION   DATLBZ010000091 DATLBZ010000000
VERSION     DATLBZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12693
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3018759"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VMZ53_04870"
     CDS             complement(<1..300)
                     /locus_tag="VMZ53_04870"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HUS27812.1"
                     /translation="MGLALGIAFVFGGAYWAYTKHEWAQHAGHADGTVIATPHGGAHP
                     EVAYNTPEGGYQNKPMSDITNSSYDGEHVHVLYDRKQPSSAELDSWPNLYLFATIF"
     gene            complement(328..3090)
                     /locus_tag="VMZ53_04875"
     CDS             complement(328..3090)
                     /locus_tag="VMZ53_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27813.1"
                     /translation="MTPGMMVAAKYELIRELGRGGMGVVYAARDLKLGRRVAMKFLRH
                     VDREVLDRFLIEARATAQCNHDNIVIIYEVDEYAGMPYMVLEYLEGRSLRDFMGPFGQ
                     GDPMPASRVVELIIPVARALARASELGIVHRDLKPENVIVTHNGQVKVLDFGIAKALG
                     TPEVKPRLSSQSGNSDMTLTREGAMVGTLPYMSPEQMGMDYIDGRSDLFSLGMIMYEM
                     LSGRHPVEPLTADALTQNLINAAPMPSVRNAAPEIAEPLAKVVDDLLKKRKHERLPSA
                     TELVRRLEQLLPGRGGRELAEGESPFPGLTPFQEADADRFFGRGRDVQRMVARVRELP
                     LTGIVGPSGVGKSSFVRAGVGPALKASGEHWDVVTLRPGRQPLAALASIVERFTTRAM
                     GRGAGDSLTGADHTHLMTRLRTEPGYLGSLLRTRASASRTNLLLFVDQFEELYTLVTD
                     IDERRAFTAALAAVADDTAAPLRVIVSMRSDFLDRVGEDPRFLEELSRGLVFLSAPDR
                     SGLREAIIAPLEMVGCELESENMLDHMLDELEGTPGALPLLQFTAAKLWDLRDRERKL
                     LTVASYEAIGGMTGALATHADEVVRNMNANAQRLTQKVFRQLVTPDRTRAIVELSELS
                     QLSADRAEISRVIDLLVAARLLVVQTRGDTGGGSVEIVHESLLDRWPMLRRWLDEDQE
                     DAAYLQDIGAAAKQWDAKGRPAGLLWTGDAMLEARRWYGSRPRQLPPREQAFIDAVLA
                     HARSAQRTRRILLAAAFIVLAVFGAVAGVGYLRVKSAEERATDALATAQTDRDKAQAA
                     LAARDAADAERAKALGEKQAALSDAERAKQLAEEEERKKLEAQAGEMMSREELVKKNA
                     ELQQSLADQKAARDRAEAATKEAKTAAAEAQRLQKELQVKLDEEKARVKQLQEEMKKI
                     STKLKD"
     gene            3255..5657
                     /locus_tag="VMZ53_04880"
     CDS             3255..5657
                     /locus_tag="VMZ53_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27814.1"
                     /translation="MGRAALLVSVLFAGCAESSSITCDDGTTCPGGFQCDIEHHRCLL
                     PAQVIACEGKADGDDCTYNNMPGACQFGACETFYCGDGRIVAPEQCEPGFEDAPDDLG
                     MNENGEQMSCIDLGYYEPAGLACDRQTCRYKQDACGGGRCGDAELNGPELCDGPTNET
                     CVSIGFDAGSVSCNALCGFNVLDCSRFGWNPESIADVQALAVAGSGPGDQWAVGEGGK
                     AMHYEGAFWNTYPTGVINPLVGAWSNGPDDTWAVGLGFNSANPSVILHWTGKLNGWSK
                     VTGQPAGEYVDVSGAGTNVYVATSGSGVLHYNGATWAELGNLTVGVVRISAVSTSNVY
                     AATAPGPLAQWNGSTWTTLTDAALAGVKFEFLDANDTNDVWAAGHLSATPGTGVIAHY
                     DGAWRVWKNAGESYNNIAASAPNDAWVAGVDGIMRHWDGAAWSRSTNIGASPSGLTAI
                     SGFISLSGTEVLAVSTLRLAYRYRGQAYGTYKPLGSNPFDATPNNAMYGTAADNQYVV
                     NAKGEVWRFDGNTWTNVHSISGDPNSPIPAKAIHGSGANDIWVGGDNGTMHHWNGATW
                     SNETAATTPIRKVWVAAAGGDVWAFTDSLAFHREGTPVMWKSYALGGATILSVSGTAA
                     NDIWVVDGGSPHKLWHWTGGTAWSEVLVTTADTALLAVVAITATDVHVTGVNGRMNHF
                     NGATWSQQTVTPLAELTMLAATGPHDVIAASQRDLFHFNGLEWSNVRPPVDFVPNTSD
                     YLPIVGLQAFPGHIDMLLDRYRIRTLIRTRALRCEIHEVCGDSVDNNCNGDIDTSDAE
                     CQ"
     gene            complement(5658..6002)
                     /locus_tag="VMZ53_04885"
     CDS             complement(5658..6002)
                     /locus_tag="VMZ53_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27815.1"
                     /translation="MRAWIALLLIAGCGAAPNQPVLANAPRPNPAAVAGVAAGAAAAI
                     TLADPDAATRGPEKKKESTDMKGQKVKENVPAAVLDRADHQNDAKQPKRDPANPAASP
                     EQPKGALDFGSP"
     gene            6027..7004
                     /locus_tag="VMZ53_04890"
     CDS             6027..7004
                     /locus_tag="VMZ53_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HUS27816.1"
                     /translation="MRAIAISKPGGPEVLALVERASPEPSRGEVLVRVRGTAVNRADL
                     LQRMGAYPAPADSPPDIPGLEIAGEVAALGRGVERLKVGDRVFGLVGGGGYAEEVVSH
                     ERALARIPDGMSFEDAAAIPEAYCTAHDAIVTQAQIKSGETLLVHAVGSGVGTAAVQL
                     ARALGATVIGTARTADKLDRARELGMDHGIVAEGGKFADAVRGVASDGVSVILELVGG
                     NYVAEDLRCVATLGRIVLVGLMAGTKGDLDLGLVLRKRARIFGTVLRARPLEEKIAAM
                     RAFEAQVVPLVARGACKPIIDCVMPLADAARAHERMQSNAGFGKIVLTC"
     gene            complement(7013..7654)
                     /locus_tag="VMZ53_04895"
     CDS             complement(7013..7654)
                     /locus_tag="VMZ53_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27817.1"
                     /translation="MRSALAFLLFASAAAACTSAAPTDTDSFDSVRDRLATKPTRLYI
                     GSAGSSGTITAKRWTADGWVEGQTPVDISSGELRASVDHTGALKLDAFEVGVAPIDIP
                     EEVFKKPAQLKDVRVKLTNSVAGQTTWTSDDDATVALTLELDLDWSIAVNGAQTPLGT
                     QHLPPVPVDVTLTGAGDHVDASIGLHASGELWDWAGLLKVTGLELALSAETTD"
     gene            complement(7949..9571)
                     /locus_tag="VMZ53_04900"
     CDS             complement(7949..9571)
                     /locus_tag="VMZ53_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS27818.1"
                     /translation="MKNGRTIANDSGSTTSTWMSEKVPSFDEEISDGARPDVCVIGAG
                     IAGLSVALSLVQEGLDVLVIDQGPIGGGQTARTSAHLASALDDHFYVLEKHFGRSGAK
                     MAAESHSQAIDTIEANTRTFAIDCQFRRVDGYLWAPDAEPNGHAKSLPHELKKELEAA
                     GRAGLSVEEVAKAPLPFDTGPALRFAMQAEFHPLQYIRGIAEAIVKGGGRIATHCHVA
                     NVKDGSPVEIELKSGRKIQATAVVDATNMSITSRINIPTREASYRTYCIGVEVPLGYV
                     PHGLYWDTLDPYHYIRVAKGDMDREILIVGGEDHRVGQGDPEVHFPRLEAWLRKHFPE
                     AGPAVATWSGQVQEPHDGMAYIGRLPHHEHVFVVTGDSGNGLTHGVVAGLMLPALIQG
                     REHAWSKIYSPSRTRLNGITTMAHEAAKSNAPYVDWMRGGDVESIDDIQPGHGATIRR
                     GLHVIAAYKDEHGQCHLKNARCTHLSGVVRWNEVEKSWDCPCHGSRFDAYGRVINGPA
                     IADLDAAPADIESPSEIPSPVLDDNLIPLRTT"
     gene            complement(9619..9837)
                     /locus_tag="VMZ53_04905"
     CDS             complement(9619..9837)
                     /locus_tag="VMZ53_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27819.1"
                     /translation="MRNTDSFPTIGFDHLADVTGGCGKKKCAPPPPPPQQMAAPQPQS
                     DRGVDVTVATGAAAAQLIGGGAPQIQRA"
     gene            complement(9953..10360)
                     /locus_tag="VMZ53_04910"
     CDS             complement(9953..10360)
                     /locus_tag="VMZ53_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27820.1"
                     /translation="MDRLSIEQTGTFEFALWCKLLRDRGYTGMQVARVLGKSEGYVNN
                     LVRILERASLKILERWCAEQRPNAATLHVCATDWLMHVCILPHAEQDVLLAERVAKLT
                     RAPTNTNAAAVAPDPDTPAVTPAPNGVSADNAE"
     gene            10532..10702
                     /locus_tag="VMZ53_04915"
     CDS             10532..10702
                     /locus_tag="VMZ53_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27821.1"
                     /translation="MPMLIGAVVADVVVTSVIASQIEDFTVGATIGTTLAVTAVDVTI
                     GCFLGACAPLRL"
     gene            10746..11513
                     /locus_tag="VMZ53_04920"
     CDS             10746..11513
                     /locus_tag="VMZ53_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27822.1"
                     /translation="MYRDSANALDERDRAVGAILVGEALVDEIKRAHEQIAASDSPLS
                     HRERWRTITSVHDTYPHVWQHLDRAREVLAKRGANTLRYDELRPQAKRAPTNAEGSID
                     IAALDDVRRAIEDLKLAVPGADWKAINSRTQGLIHAKLSRPRGQRAIVTAALAMFLFG
                     VVAWAIAIMPEHKVNRREAMRRELSQIQQIRKVKIDVTRVELGARCDVDRARELTKLL
                     AMDGRTEEARTFGATYMLRCGDDSVVDNWAHAPHPNH"
     gene            11560..>12693
                     /locus_tag="VMZ53_04925"
     CDS             11560..>12693
                     /locus_tag="VMZ53_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HUS27823.1"
                     /translation="MRLALALLLIGCHSSARPKLPLVTVGEASQFTKTGHYDEAITLC
                     HDFARAYEGVRCDEIGRTLEDRPIVALHVSRGANRPTIYLQGGIHAGEIDGKDAGFWF
                     LRDLLDGKVAQGALDHVDIEFVPVINPDGHERFSPNNRPNQRGPAEMGFRTNAARMNL
                     NRDYVKADSLEIRAVLGVFRKYVPIVSVDMHVTDGAKFEHDVAVLVGTFAKRDDGLDE
                     LSRTLSDQLDARLTALGHLPLAFYPSFEKDDDPASGFSVGEAPLRFSQSYASARSRIG
                     ILLETHSWRPYKHRVETTYHVLQALFEQAAKDAATWAKVEADAVAADQKLLGGQLPVA
                     YENSTHVTQLQFRGYAYEQRASEISGGTWTVYDETKPQIWNIPL"
BASE COUNT         2072 a         4091 c         4277 g         2253 t
ORIGIN      
        1 gaagatcgtt gcgaacagat acaagttcgg ccagctatcg agctcggcac tgctcggttg
       61 cttgcggtcg tagaggacgt gcacgtgctc gccgtcgtag ctcgagttgg tgatgtcgct
      121 catcggctta ttctggtacc cgccttcagg cgtgttgtac gcgacctcgg gatgcgcacc
      181 accgtgcggc gtcgcgatga cggttccatc ggcgtggccg gcgtgctgtg cccactcgtg
      241 ctttgtatac gcccagtacg cgccgccgaa cacgaacgcg ataccaagcg cgaggcccac
      301 cagcggctcg ttttgcgcca tcgcggacta gtccttcagc ttcgtcgaga tcttcttcat
      361 ctcttcttgc agctgcttca cgcgcgcctt ttcctcgtcg agctttactt gcagctcctt
      421 ctggaggcgc tgcgcttcgg cggccgcggt ctttgcttct ttggtcgcgg cttcggcgcg
      481 gtcgcgcgcg gctttttgat cggcgagcga ttgctgcagc tcggcgttct tcttgacgag
      541 ctcttcgcgc gacatcatct cgcccgcttg tgcctcgagc ttcttgcgct cttcttcttc
      601 ggcgagctgc ttggcgcgtt cggcatcgga gagcgcggcc tgtttttcgc cgagagcttt
      661 ggcgcgctcg gcatcggcgg catcgcgagc tgcgagtgcg gcttgcgctt tgtcgcggtc
      721 ggtttgggcg gtggcgagcg catcggttgc gcgctcttcg gcggatttga cgcggaggta
      781 gccgacaccc gcgacggcgc cgaacaccgc gaggacgatg aacgcggcgg cgagcaggat
      841 gcggcgcgtg cgctgcgcgc tgcgggcgtg agcgaggacc gcgtcgatga atgcttgctc
      901 gcgcggtggg agctggcgcg ggcgtgagcc ataccagcgg cgcgcttcga gcatcgcgtc
      961 gcccgtccag agcaggcctg ccgggcggcc tttcgcgtcc cactgttttg cggccgcgcc
     1021 gatgtcttgc aggtaggccg cgtcttcttg gtcctcgtcg agccagcgcc ggagcatcgg
     1081 ccagcgatcg agcagcgact cgtggacgat ctcgacggag ccgccgcccg tgtcaccgcg
     1141 cgtttgaacg acgaggaggc gtgcggcgac gagcagatcg atcacgcgag agatttcggc
     1201 gcggtcggcg ctgagctgag agagctcgga cagctcgacg atcgcgcgcg tgcggtctgg
     1261 cgtgacgagc tggcggaaga ctttctgcgt gaggcgctgc gcgttcgcat tcatgttgcg
     1321 tacgacttcg tcggcgtggg tcgcgagagc gccggtcatg ccaccgattg cttcgtagct
     1381 cgcgacggtg agcagcttcc gctcgcgatc gcgcaggtcc cacagcttgg cggcggtgaa
     1441 ctgcaggagc ggcagcgcgc ccggcgtgcc ttccagctcg tcgagcatgt ggtcgagcat
     1501 gttctcgctc tcgagctcgc agccgaccat ctcgagcggc gcgatgatcg cctcgcgaag
     1561 gccgctgcga tcaggggcag acaagaacac gaggccgcgg gacagctcct cgaggaagcg
     1621 cgggtcttcg ccgacgcgat cgaggaagtc cgaccgcatc gagacgatca cgcgcagcgg
     1681 tgctgcagtg tcgtccgcga ccgcggcaag cgcggcggtg aatgcgcggc gttcgtcgat
     1741 gtccgtgacg agcgtgtaaa gctcttcgaa ttgatcgacg aacaacagca agttcgtgcg
     1801 cgacgccgat gctcgcgtgc gcaagagcga gccgagatag cccggctccg tccggaggcg
     1861 cgtcatcaag tgcgtgtgat cggcgccggt gagggaatcg cccgcgcctc ggcccatcgc
     1921 gcgcgtcgtg aaccgctcga cgatgctcgc gagtgcggcg agtggctggc gacctggcct
     1981 gagcgtgacg acgtcccagt gctcgccact cgctttgagc gcgggaccga cgccggcgcg
     2041 cacgaacgat gatttgccaa cgcccgacgg gccgacgatg ccggtgagcg gaagctcgcg
     2101 cacgcgcgcg accatgcgtt gaacatcgcg accgcggccg aagaagcgat cggcgtcggc
     2161 ttcctggaat ggcgtgagac ccgggaacgg gctttcacct tcggccagct ctcggccacc
     2221 gcggccgggg agcagttgct cgaggcgacg cacgagctcg gtcgcgctcg gcaagcgctc
     2281 gtgcttgcgc ttcttgagga gatcgtcgac gaccttcgcg agcggttcgg caatctccgg
     2341 cgcggcgttg cgtaccgacg gcatcggcgc cgcgttgatc agattttgcg tcagcgcgtc
     2401 ggcggtgagc ggctcgacgg gatgccggcc cgacagcatc tcgtacatga tcatgccgag
     2461 cgaaaacaga tccgaccggc cgtcgatgta gtccatgccc atttgctcgg gcgacatata
     2521 gggcagcgtg ccgaccatcg cgccttcgcg cgtcagcgtc atgtcgctgt tgcccgactg
     2581 cgacgagagg cgcggcttca cctcgggcgt gccgagcgcc ttcgcgatgc cgaagtcgag
     2641 gactttgacc tgaccgttgt gcgtgacgat cacgttctct ggcttgagat cgcgatgcac
     2701 gatgccgagc tccgacgcgc gagcgagcgc gcgggcaacc ggaatgatca gctcgacgac
     2761 gcgcgacgcg ggcatcggat cgccttgccc gaacggcccc atgaaatcgc gcagcgaccg
     2821 gccctcgaga tactcgagca ccatgtacgg catgcccgcg tactcgtcga cctcgtagat
     2881 gatgacgatg ttgtcgtgat tgcactgcgc ggtcgcgcgg gcctcgatga ggaagcggtc
     2941 gagaacctcg cgatcgacgt gccgcaagaa cttcatcgcg acgcgtcgac cgagcttgag
     3001 atcgcgcgcg gcgtaaacga cgcccatgcc gccgcggccg agctcgcgaa tgagctcgta
     3061 cttcgcggcc accatcattc ccggcgtcaa gtccgtcccg ggtagcgacg aggatggctc
     3121 tgagagaaat ttcgctcttg gagtggtacc gctgcgggga gatgtaggcg ttacggggcc
     3181 cacaccgaac tgcgtacctg tcacgcgttc gtcgctcggc acacgtcgag ggtaacacgc
     3241 ggtagcatgg tttcatgggt cgagctgcgc tcctcgtgag cgtgttgttc gcaggctgcg
     3301 ccgagtcgtc gtcgatcaca tgcgatgacg gcacgacgtg ccccggcggc tttcagtgcg
     3361 atatcgagca tcaccgctgc ttgctgcccg cgcaggtcat tgcgtgcgaa ggcaaagcgg
     3421 acggcgatga ctgcacgtac aacaacatgc ccggcgcatg tcagttcggc gcgtgcgaga
     3481 cgttctactg cggcgatggt cgcatcgtcg cgccggagca gtgcgagccc ggcttcgaag
     3541 acgcgcccga cgatctcggg atgaacgaga acggcgagca gatgagctgc atcgacctcg
     3601 gctattacga gcccgcaggt ctcgcgtgcg atcgccagac gtgtcgctac aagcaagacg
     3661 catgcggcgg cggtcgttgc ggcgacgccg agctcaacgg tcccgagctg tgcgacggcc
     3721 cgacgaacga gacgtgtgta tcgatcggct tcgacgccgg ctctgtcagc tgcaacgcgt
     3781 tgtgcggctt caacgtcctc gactgcagcc gcttcggttg gaatcccgaa tcgatcgcgg
     3841 acgtgcaggc gctcgcggtg gccggcagcg gtcccggcga tcagtgggcc gtcggcgagg
     3901 gcggcaaggc gatgcactac gagggcgcgt tctggaacac gtatccgacg ggcgtgatca
     3961 atccgctcgt cggcgcatgg tcgaacggcc ccgatgatac gtgggcggtc ggcctcggct
     4021 tcaacagtgc gaacccgtcg gtgattctgc attggacggg caagctcaat ggctggtcga
     4081 aggtgacggg ccagcccgcc ggcgaatacg tcgatgtgtc gggtgcgggc acgaacgtct
     4141 acgtcgcgac gagcggcagc ggcgtgttgc actacaacgg ggcgacgtgg gcggagctcg
     4201 gtaacctcac cgtcggcgtc gtgcggatct ccgccgtgtc gacgtcgaac gtgtacgccg
     4261 cgacggcacc gggcccgctc gcccagtgga acggctcgac gtggacaacg ctcaccgatg
     4321 cagcgctcgc cggcgtgaag ttcgaattcc tcgacgcgaa tgacacgaac gacgtgtggg
     4381 ccgccggtca tctgagcgcg acgcccggca cgggtgtgat cgcgcactac gacggcgcgt
     4441 ggcgcgtctg gaaaaatgcg ggcgagagct acaacaacat cgcagcgtcc gcaccgaacg
     4501 acgcatgggt cgcgggcgtc gacggcatca tgcggcactg ggatggcgcg gcgtggtcgc
     4561 gttcgacgaa catcggtgcg agcccgagcg gcctcaccgc gatctccggc ttcatcagcc
     4621 tgtcgggcac cgaggtcctc gccgtctcga cgctgcgcct cgcgtatcgt tatcgcggtc
     4681 aggcgtacgg cacgtacaag ccgctcggca gcaatccatt cgacgcgacg ccgaacaacg
     4741 cgatgtacgg caccgccgct gacaaccaat acgtcgtcaa cgccaagggc gaagtctggc
     4801 gcttcgacgg caacacctgg acgaacgtcc actcgatcag cggcgacccg aacagtccga
     4861 tccccgcaaa ggcgatccac ggctctggcg cgaacgacat ttgggtcggc ggcgacaacg
     4921 gcacgatgca tcactggaac ggcgcgacgt ggtcgaacga gaccgcagcg acgacgccga
     4981 tccgcaaggt gtgggtcgca gccgcgggcg gtgacgtgtg ggcattcacg gattcgctcg
     5041 cgtttcatcg cgaaggcacc ccggtgatgt ggaagtcgta cgcgctcggt ggcgcgacga
     5101 ttctctccgt atcgggcacc gcggccaatg acatctgggt cgtcgacggc ggctcgccgc
     5161 acaagctgtg gcattggacg ggcggcacgg cgtggagcga agtgctcgtg acaacggccg
     5221 acaccgcgct gctcgcggtc gtcgctatca cggcgacgga cgtgcacgtc accggcgtga
     5281 acgggcgcat gaaccacttc aacggcgcga cgtggtcgca gcaaaccgtg acgccgctcg
     5341 ccgagctgac gatgctcgcc gcgacgggtc cgcacgacgt gatcgccgcg agccagcgcg
     5401 acttgttcca cttcaacggc ctcgagtggt cgaacgtgcg gccgcccgtc gacttcgtgc
     5461 cgaacacctc cgactacctg ccgatcgtcg ggctgcaggc gttcccaggt cacatcgaca
     5521 tgctgctcga tcgctatcgc atccgcacgc tgatccggac gcgcgccctg cgctgcgaga
     5581 tccacgaagt gtgcggcgac agcgtcgaca acaactgcaa cggcgacatc gatacgtccg
     5641 acgccgagtg tcagtagcta cggcgatccg aagtcgagcg cgcctttcgg ctgctccggc
     5701 gacgcggctg gattcgcagg gtcacgcttc ggctgcttcg cgtcgttctg atggtcggcg
     5761 cgatcgagga cagcggccgg cacgttctcc ttcaccttct ggcccttcat gtcggtgctc
     5821 tctttcttct tctcgggccc tcgcgttgcg gcatctggat cggcgagcgt gatcgcagcg
     5881 gccgctccgg ccgcgacacc cgcgaccgcc gccggattcg ggcgaggtgc gttcgcgagc
     5941 acgggttgat tcggcgccgc gccgcaaccc gcgatcagga gcagtgcgat ccacgcacgc
     6001 atgatcgaat ggtacacccg tgcgcgatgc gagccatcgc gatttcaaag cccggcggac
     6061 ccgaagtgtt ggcgctcgtg gagcgcgcgt cgcccgagcc atcacgcggc gaggtactcg
     6121 tgcgggtgag aggcaccgcg gtcaatcgcg cggatctgct ccagcgcatg ggcgcctacc
     6181 ctgctcccgc cgattcgccg cccgacattc cgggcctcga gatcgcgggc gaggtcgcgg
     6241 cgctcgggcg gggcgtggag cggctgaaag tcggcgaccg cgtgttcggt ctcgtcggcg
     6301 gcggtggcta cgccgaggaa gtcgtcagtc acgaacgcgc gctcgcgcga attccggacg
     6361 gcatgagctt cgaagacgcg gccgcgattc ccgaggcgta ttgcaccgcg cacgacgcga
     6421 tcgtcacgca agcgcagatc aagagcggcg agacgctgct cgtgcacgcg gtcggcagcg
     6481 gcgtcggtac ggcggccgtt cagctggcac gcgctctcgg cgcaacggtg atcggcaccg
     6541 cgcgcaccgc cgacaagctc gatcgtgcga gagagctcgg catggatcac ggcatcgttg
     6601 ccgagggcgg caagttcgca gacgcggttc gcggcgtcgc gtccgacggc gtgtcggtca
     6661 tcctcgagct cgtcggcggc aactacgtcg ccgaagatct gcgctgcgtc gcgacgctcg
     6721 gtcgcatcgt gctcgtcggt ctgatggcgg gcacgaaggg cgacctcgat ctcggcctcg
     6781 tgctgcgcaa gcgcgctcgc atcttcggca ccgtgctgcg cgcgcgaccg ctcgaagaga
     6841 agatcgccgc gatgcgcgcg ttcgaagcgc aggtcgtacc gctcgtcgca cgcggcgcgt
     6901 gcaagccgat catcgactgc gtgatgcccc tcgccgacgc tgctcgcgcg cacgagcgca
     6961 tgcagagcaa cgcgggcttc ggaaaaatcg ttctgacttg ctagcccgcg gcctagtctg
     7021 tggtctctgc cgagagcgcg agctcgaggc ccgtgacctt cagcagtccg gcccagtccc
     7081 acagctcgcc cgacgcgtgc aggccgatcg atgcatcgac gtgatcgcct gcgcccgtta
     7141 gcgtgacgtc caccggcacc ggcggaaggt gttgcgtgcc gagcggcgtc tgcgcgccgt
     7201 tgaccgcgat cgaccagtcg agatcgagct cgagcgtgag cgcgaccgtc gcgtcgtcat
     7261 cgctcgtcca cgtcgtctga cccgcgaccg aattcgtcag cttcacgcgg acgtccttca
     7321 gctgcgccgg cttcttgaag acttcttctg ggatgtcgat tggcgcgacg ccgacctcga
     7381 atgcatcgag cttcaatgcg ccggtgtgat cgacggatgc gcggagctcg ccgctagaga
     7441 tgtcgacggg cgtttggcct tcgacccaac cgtccgcggt ccagcgcttc gcggtgatgg
     7501 tgccgctgct gccggcggag ccgatgtaga ggcgcgtcgg cttggtcgcg aggcgatcgc
     7561 gaaccgagtc gaacgagtcc gtgtccgtgg gcgcagcaga cgtacaggcc gccgctgccg
     7621 aagcgaagag aagaaaagct aaagcggaac gcataataac gcggtgcgta gcaattgtta
     7681 cgccacgggt acgacatgta ggaatcgccg gcctcacgcg gtattgaaca acgtacaata
     7741 gctgccgcac tgcgccaaac cgtcgcgatc gcgcgccgag tggcagctcg actggccgct
     7801 gactcaacgc gtcaggttgg tcagaacctg ttgtccggca ggcggatacc gacctaaccc
     7861 gctattgaac cgttcccaac aggtcacgga ccgcgaacac gttaccgcgc ggtaattcgg
     7921 tccgacctat tttctgcgag ccgggtgatc aggtcgtccg cagcgggatg agattgtcgt
     7981 cgagcaccgg cgacggaatc tcgctcggcg attcgatatc ggcgggcgct gcatcgagat
     8041 cggcgatcgc cggcccgttg atcacgcggc cgtaggcgtc gaaccgcgac ccgtggcacg
     8101 ggcagtccca gctcttctcg acttcgttcc agcgcacgac gcccgagaga tgcgtgcagc
     8161 gcgcgttctt caggtggcac tggccgtgct cgtctttgta cgcagcgatc acgtggaggc
     8221 ctcgacggat cgtcgcgccg tgccccggct ggatgtcgtc gatcgattcg acatcgccgc
     8281 cgcgcatcca atcgacgtac ggcgcgttcg acttcgccgc ttcgtgcgcc atcgtcgtga
     8341 tgccgttcag gcgcgtgcgg ctcggcgagt agatcttcga ccacgcgtgc tcgcgtcctt
     8401 ggatcagcgc aggcagcatg agacccgcga cgacgccgtg cgtgagcccg ttgccggagt
     8461 cgccggttac gacgaagacg tgctcgtgat gcggcagcct gccgatgtac gccatgccgt
     8521 cgtgtggctc ctgcacctgt cccgaccatg tcgcgaccgc cggtcccgct tcgggaaaat
     8581 gcttccgcag ccacgcttcg aggcgcggga agtgaacttc tggatcgcct tggccgacgc
     8641 gatgatcttc gccgccgacg atcaggatct cgcgatccat gtcgcccttc gcgacgcgaa
     8701 tgtagtgata cggatcgagc gtgtcccagt agaggccgtg cggaacgtag ccgagcggca
     8761 cttcgacgcc gatgcagtac gtgcgatacg acgcttcgcg cgtcgggatg ttgatgcgac
     8821 tcgtgatgct catgttcgtc gcatcgacga ccgccgtcgc ttgaatcttg cggccgctct
     8881 tcagctcgat ctcgacgggc gagccgtcct tcacgttcgc gacatggcag tgcgtcgcga
     8941 tgcgcccgcc acctttgacg atcgcctcgg cgatgccacg gatgtactgg agcggatgaa
     9001 actcggcctg catcgcgaac cgcagcgccg gaccggtgtc gaacggaagc ggcgccttcg
     9061 cgacttcttc gacgctgagc cccgcgcggc cagcagcctc gagctccttc ttcagctcgt
     9121 gcggcaggct cttagcatgg ccattcggct cggcatcggg agcccagaga tagccatcga
     9181 cgcgacggaa ctggcagtcg atcgcgaacg tgcgcgtgtt cgcttcgatc gtatcgatcg
     9241 cttgcgagtg actctcggcg gccatcttcg cgcccgagcg accgaagtgt ttctcgagca
     9301 cgtagaagtg gtcgtccaac gccgatgcga ggtgcgcgct cgtccgagcg gtctgtccac
     9361 cgccgatcgg gccctgatcg atcacgagca cgtcgaggcc ttcctgaacg agcgaaagcg
     9421 cgacgctgag ccctgcgatc cctgcgccga ttacgcagac atcggggcga gctccgtcgg
     9481 aaatctcctc atcgaagctc ggaactttct cggacatcca ggtcgacgtc gtcgatccag
     9541 aatcattcgc gatggtacga ccgttcttca tgcgtcctaa cgcagcaagc gataggccac
     9601 cgtcgcgatc tcagacgttc acgcgcgttg gatctgcggc gcgccaccgc cgatcaactg
     9661 cgccgccgca gcgccggtcg cgacggtcac gtcgacgccg cgatcgctct gcggttgggg
     9721 cgcggccatc tgctgcggcg gtggcggcgg cggcgcgcat ttcttcttcc cgcagccgcc
     9781 ggtgacgtcg gcgaggtggt cgaatccgat cgtggggaac gagtccgtgt tgcgcatgct
     9841 cgcttgctac agcagccgat gtgccagcgg ctgcgccaat cgtgtcgcgc ggttagcaag
     9901 cggcgcgtga gtccgcgagt aggcagcctg tcgccgcgcg ttcgctgccc cgctattccg
     9961 cgttgtccgc actcacgccg tttggcgcag gcgtgaccgc tggcgtgtcc ggatctggag
    10021 cgacggcggc ggcgttcgtg ttcgtcggcg cgcgcgtgag ctttgcgacg cgttcggcga
    10081 gcagcacgtc ttgctcggcg tgcggcagga tgcacacgtg catcagccag tcggtcgcgc
    10141 acacgtgaag cgtcgcggcg ttgggccgtt gttcggcgca ccaccgctcg aggattttca
    10201 acgacgctcg ttccaggatg cgaacgagat tgttgacgta gccctcgctc ttgccgagta
    10261 ctcgcgcgac ctgcattccc gtgtagccgc ggtcgcgcag cagcttgcac cagagagcga
    10321 attcgaaggt tccagtttgt tcgatcgaga gtctgtccac gacgaccctc ctgccgaaca
    10381 agacgcgcga aacatgcgac ttcttcccgc gttatgatgg cgtatgccac gccgcgctcc
    10441 cgcgcctgcc atcgtctgct ccctcgcgtt agcgctggca atgggcgggt gtgtgacagg
    10501 agtaggcgtc tggaagcgtc aggagacgcg gatgccgatg ctgatcgggg cggtcgtggc
    10561 ggacgttgtg gttacgagcg tgatcgcgtc gcagatcgag gacttcacgg tgggggcgac
    10621 gattgggacc acgcttgccg tgaccgcggt cgatgtcacg attggctgtt ttttgggcgc
    10681 ctgcgcgcca ttgcgcctgt gatttcgcct tgccaggttc gggcgaatcc gaaacactgg
    10741 aagggatgta tcgcgattcg gctaacgccc tcgacgaacg cgacagagcc gtcggcgcga
    10801 tcctcgtcgg cgaagcgctg gtcgacgaaa taaagcgggc gcacgagcaa atcgctgcat
    10861 ccgactcgcc gctctcgcat cgagagcgtt ggcggacgat cacgagcgtt cacgatacgt
    10921 atccgcatgt gtggcagcac ctcgatcgcg cacgtgaggt gctcgcgaaa cgcggcgcga
    10981 acacgctgcg ctatgacgag ctccgtccgc aggcgaagcg ggcgccgacg aatgcggaag
    11041 gcagcatcga tatcgccgca ctcgatgacg tgcgccgcgc gatcgaagac ctcaagctcg
    11101 cggttccggg cgccgactgg aaggcgatca actcgcgcac gcagggcctg attcacgcga
    11161 agctctcgag gccgcgtggc cagcgcgcga tcgtgactgc cgcgctggcg atgtttctgt
    11221 tcggcgtcgt cgcgtgggcg atcgcgatca tgccggagca caaagtgaac cgccgcgaag
    11281 cgatgcgtcg cgagctgtcg cagatccagc agatccgcaa ggtgaagatc gacgtcactc
    11341 gcgtcgagct cggagctcgc tgcgatgtcg atcgcgcgcg cgagctgacg aagctcctcg
    11401 cgatggacgg ccggaccgaa gaagcgcgaa cgttcggcgc gacgtacatg ctgcgctgtg
    11461 gcgacgattc ggtcgtcgac aactgggcgc acgctccgca tccgaatcac tagtcgctga
    11521 atctgctgca ccggcggtgc gcgtgtagat tcgggcgcga tgcgtctggc tctcgcgttg
    11581 ctcctgatcg gatgccactc gtccgcacga ccgaagctgc cgctcgtgac ggtcggcgag
    11641 gcgtcgcagt tcacgaagac gggccactac gacgaagcca tcacgctgtg ccacgacttc
    11701 gcgcgcgcct acgaaggtgt tcgctgcgac gagatcggcc gcacgctcga ggatcggccg
    11761 atcgtggcgc tgcatgtctc gcgcggagcg aaccggccaa cgatctatct acagggcggg
    11821 attcatgccg gcgagatcga tggcaaggac gcaggctttt ggtttctgcg cgatctcctc
    11881 gacggcaaag tcgcacaagg cgcgctcgac cacgtcgaca tcgagttcgt gccggtcatc
    11941 aatcccgatg gtcacgagcg gttcagcccg aacaaccggc cgaatcagcg cggccccgcc
    12001 gagatgggct ttcgcacgaa tgccgcgcgg atgaatctca accgcgacta cgtcaaggcc
    12061 gactcgctcg agatccgggc cgtgctcggc gtgttccgca agtacgtgcc catcgtgtcg
    12121 gtcgacatgc atgtcaccga cggcgcgaag ttcgagcacg acgtggcggt tctcgtcggc
    12181 acgtttgcca agcgcgacga tggtctcgac gagctttcgc gcacgctctc cgatcagctc
    12241 gacgcgcgcc tcaccgcgct cggccacttg ccgctcgctt tctatccgtc gttcgagaaa
    12301 gacgacgacc cggcgtcggg cttctccgtc ggcgaagcgc cgctgcggtt ctcgcagtcg
    12361 tatgcgagcg cgcgctcgcg catcggcatt ctgctcgaga cgcacagctg gcgtccgtac
    12421 aagcatcgcg tcgaaacgac gtatcacgtg ttgcaagcac tattcgagca agcggcgaag
    12481 gacgcggcca cgtgggcgaa ggtcgaggcg gatgctgtcg ctgcggatca gaagcttctc
    12541 ggcgggcagc tcccggtggc ctacgagaac agcacgcacg tcacgcagct gcagtttcgc
    12601 ggctacgcgt acgagcagcg cgcgtcggag atttcgggcg gtacgtggac tgtgtacgac
    12661 gagacgaagc cgcagatctg gaacatcccg ctc
//
LOCUS       DATLBZ010000092         6667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6000223, whole genome shotgun sequence.
ACCESSION   DATLBZ010000092 DATLBZ010000000
VERSION     DATLBZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6000223"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1128)
                     /locus_tag="VMZ53_04930"
     CDS             complement(169..1128)
                     /locus_tag="VMZ53_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS27824.1"
                     /translation="MDPAIDVRDLRKTYRTPLRRKKVEALRGVTFSVERGQIFGFVGP
                     NGAGKTTTIRTLMGLIRPTGGSAKILGHEIPSRAARNRLGFLPESPYFYDYLTVGELL
                     DLAGRLFGVPRDVRKKRANELIEKVGLGRAREQNLKKFSKGMLQRAGLAQALMNDPDL
                     VVLDEPMSGLDPIGRKEIRDLIVELREQGKTVFFSSHILTDVEAIADHVAIIARGSLQ
                     ATGKPSELLTKTVLGIDVTVRLPAGTELRELADRATRLRRTDDELSLTLPADTDIDAW
                     LAGARELGAKLVSVTPRHETLEEMFLREIANADKSSAATEGRV"
     gene            complement(1175..1957)
                     /locus_tag="VMZ53_04935"
     CDS             complement(1175..1957)
                     /locus_tag="VMZ53_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27825.1"
                     /translation="MRTSGRTVLIVTSIGLHAVVGFAFFVSSVWKIERIEAGNRPFDL
                     AMQPDPQPEPGSPEGSPAPEFKKKKKKEQQEKKVVVDTVQPEEIKKPDPSEASKAAFT
                     GANAGTNGNGLGSGAGSGSGNGLGSGTGSGSGSGEGSGSGGCPPGVDCTPTKFIPPTM
                     FGGLRKSGETQIHPPDVTKTEIIRSGKTEISARFSVCVGVDGGVTSLKLVKSSGFAAY
                     DATLTSGLRSWQYRPYMLDNHDGKPPHPVPVCGIVTFNYGLR"
     gene            2057..3316
                     /locus_tag="VMZ53_04940"
     CDS             2057..3316
                     /locus_tag="VMZ53_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HUS27826.1"
                     /translation="MTGALVVFCITYLVIASRQLHFLKLDRPAGAVVGAVAMVIVGGL
                     PMQLALDAIDLHVLLLLFGVLVIAAYLQDAQFFRLCAYLVLTRAKSARSLLFGLVLVS
                     GALSAFLVNDTVCVVLTPLVVAVVVEARLPPLPYLLALASAANVGGVVSFSGNPQNMI
                     IGAHAHETISFAQYFVLVLPVGALCLVVNAALIAWMFRRELPAGPLAERSPPRPAVDR
                     VLATKGVGALALFAILALAGVSLAGAAMCAAAVLMLVARTGARQVFTRVDWTLLAFFG
                     GLFVVVAGVAHAGVLNKIFDAAAPVIARGDATGDLLFVLFVVIASNVVSNVPLVLVAV
                     SWIPHMPDPTWGYIMLAVASTLAGNLTLFGSVANIIVMESAGPRGEVSFWRFLRYGSV
                     LTIVDLVLAFGLLFLERRAGIGDLLGL"
     gene            3397..3981
                     /locus_tag="VMZ53_04945"
     CDS             3397..3981
                     /locus_tag="VMZ53_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27827.1"
                     /translation="MRVWVAMIVLVLCNVAAAEPRASVGFRATWNTDASPPPWLMTGG
                     QWGLRLRGTTWLMLDGDGMRGEKLEHVDGSASQRTFLVLGLGLTTDVIAQGRNALRVL
                     GGGGVVVELAHERMLGTWDTADVATRPLGYVGAAFEHRFPQFWVEKRKKWMGGGEMGV
                     EVRAVRLFGDGAMTPAPGIAATSMQASLQMSAYF"
     gene            complement(3978..5057)
                     /locus_tag="VMZ53_04950"
     CDS             complement(3978..5057)
                     /locus_tag="VMZ53_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27828.1"
                     /translation="MKKFFLVVVGVVALGLALRFTLNKKPKQKADATKPPTVVVNTPP
                     PDEQPPATPDQPPPTPTTTPTPAPDEPPPPPRIAGSVQISDVQTQWFASAPGVLYYCE
                     RGTMYAQSKSGGAAVNVGDCGSAFDMRADAQGVVFCDGDGQLMRITAGADNGSQVIAG
                     GLEGCITSAIDGKWAYFVVPGFEGTENPGLFRVLRSGGTPEKIHATRPKEQFMVAVDD
                     DAVWIGAWAAGTISKLGKTPGSKARTVVTGQKGIVDLAIDGNSLYWYAEGTGEVRRRK
                     KTGGAIEVVGHDVDQEPVVVTSDGHAYWFEGKAGEDKRLVHLAPGAAKSETIASGLKA
                     PSLRADTEGVYVTELDRDGIFMFKR"
     gene            complement(5128..5592)
                     /gene="sixA"
                     /locus_tag="VMZ53_04955"
     CDS             complement(5128..5592)
                     /gene="sixA"
                     /locus_tag="VMZ53_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HUS27829.1"
                     /translation="MDVFLIRHAEAIDETLELRDPMRHLTAHGREQAVALGDRLRWHD
                     CTPTHIWTSPLVRAVQTAELVAAGLASKISVEVVPALAPDGELRSVVAMVKALAADAN
                     VVLVGHEPGLSGIGALLVGAPDFAALGKAEAARIHDGAVRWRCAWNAEAPTR"
     gene            5616..6221
                     /locus_tag="VMZ53_04960"
     CDS             5616..6221
                     /locus_tag="VMZ53_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUS27830.1"
                     /translation="MKLYGTTTSPFVRRVRVVAAEVAEPVELINTAPDEGQAELRRIS
                     PIRKVPVAVVDATVAGGLTLFDSRVIIEWLTTTHGWADLTPTRGRWHEQNIINAIDAA
                     LDAVIQLFYLRRDGVAVDGSPYAERQLARADAIFAWLGTKLVDGRSFSGGFGLPELAL
                     ISALDWMDFRKTYETSTIGAVEAVREAWRDRPSLASTMPHA"
     gene            6218..>6667
                     /locus_tag="VMZ53_04965"
     CDS             6218..>6667
                     /locus_tag="VMZ53_04965"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS27831.1"
                     /translation="MSYFTKDIMPATLHVIFGPCGAGKTTYAHALARREGAVPFVLDD
                     WGARLFGPDVEGPIDFRWMLERLGRCEAQIWLTAADVLAVGTPVVLDIGAMRRAQRER
                     IRQIAKEKGLSVQWHFIDAPQEVRRARVVDRNTAKGETFVREVPPEMF"
BASE COUNT          986 a         2307 c         2172 g         1202 t
ORIGIN      
        1 gacatcgcgc cgagcacgag cgcgaacacg ttcgcaccga cgacgagcac gaggatgccg
       61 tacagcacgc gaatgcgcgc ggccacgcga aacgtgttga gcgcgatcgc ccagatgcgc
      121 tttgccgcgc ccgtcatcgc gtgtggctcc tttgcgcaac ggcgacgctc acacgcggcc
      181 ttccgtcgcc gccgacgact tgtcggcatt ggcgatctcg cgcaggaaca tttcctcgag
      241 cgtctcgtgg cgcggcgtca ccgagaccag cttcgcgccg agctcgcgcg cgccggcgag
      301 ccacgcatcg atatcggtat cggcaggtag cgtgagcgac agctcgtcat cggtgcgacg
      361 caagcgcgtt gcgcgatcgg caagctcgcg cagctccgtg cctgcgggca gccgcaccgt
      421 gacatcgatg ccgagcacgg tcttcgtcag cagctcgctc ggcttgcccg tcgcttgcag
      481 cgatccgcgc gcgatgatcg cgacgtggtc tgcgatcgct tcgacgtcgg tgaggatgtg
      541 cgacgagaag aagacggtct tgccctgctc gcggagctcg acgatcaagt cgcgaatttc
      601 cttgcgaccg atcggatcga ggccactcat cggctcgtcg agcacgacga gatctggatc
      661 gttcatgagc gcttgcgcga gaccggcgcg ctgcagcatg cccttcgaga atttcttgag
      721 gttctgctcg cgcgcgcgac cgagcccgac cttctcgatc agctcgttcg cgcgcttctt
      781 gcggacgtcg cgtggcacgc cgaacaagcg acccgcgaga tcgagcagct cgccgacggt
      841 gaggtagtcg tagaagtacg gcgactccgg cagaaagccg aggcggttgc gtgcggcgcg
      901 gctcggaatc tcgtgaccga ggatcttcgc gctgccgccg gtcggccgga tgagacccat
      961 cagcgtgcgg atcgtcgtcg tcttgcctgc gccgttcggc ccgacgaagc caaagatctg
     1021 cccacgctcg acgctgaacg tgacgccgcg cagcgcttcg accttcttgc gacgcagcgg
     1081 cgtgcggtac gtcttgcgca ggtcgcgtac gtcgatcgcg ggatccaccg gacccgacat
     1141 taacgccaga ttcggccgcg cgcgaatcga gcggctagcg caggccgtag ttgaacgtca
     1201 cgatgccgca gaccgggacg gggtggggcg gcttgccgtc gtggttgtcg agcatgtacg
     1261 gccggtattg ccacgaccgc agcccgctcg tgagcgtggc gtcgtacgcc gcgaagcccg
     1321 acgacttgac gagcttcagc gacgtcaccc cgccgtcgac ccccacgcac acgctgaacc
     1381 gcgcgctgat ctcggtcttc ccgctgcgaa tgatctccgt cttcgtgacg tccggcggat
     1441 ggatctgcgt ttcgcccgat ttgcggaggc cgccgaacat cgtcggcggg atgaacttgg
     1501 tcggcgtgca gtcgactccg ggagggcagc ctccagagcc cgagccttcg ccggagccgc
     1561 tgcccgagcc agtccccgag ccgaggccgt tgcccgatcc gctgcctgca ccagagccca
     1621 ggccattgcc gtttgtcccc gcgttcgcgc ccgtgaatgc agccttcgag gcctcgctcg
     1681 ggtcgggctt cttgatctct tcgggctgca cggtgtccac gacgaccttc ttctcctgct
     1741 gttctttctt cttcttcttc ttgaattcgg gagcaggaga gccctcggga ctgccgggtt
     1801 cgggctgcgg gtcgggctgc atcgcgagat cgaatgggcg attgcctgcc tcgatgcgtt
     1861 cgatcttcca gaccgacgag acgaagaacg caaagccaac gacggcatgc agcccgatag
     1921 atgtgacgat caggacggtt cggccagatg tgcgcatctt ggggccaacg cagcagcttt
     1981 gctgtcgatc catgtctcgt gctgaccgcg cgcgactggt cacctcatgg gtgtcgcagg
     2041 tgtatcgtcc gatcgcgtga ctggagcgct cgtcgtcttt tgcatcacct atctggtgat
     2101 cgcgagccgc cagcttcact tcctgaagct cgaccgtccc gcgggcgcag tcgtcggcgc
     2161 ggtcgcgatg gtgatcgtcg gtgggctgcc gatgcagctc gcactcgacg cgatcgatct
     2221 ccacgtcctg ctcctgctgt tcggcgtgct cgtgatcgcc gcctacctgc aagacgcgca
     2281 gttcttccgg ctctgcgcgt acctcgtgct gacgcgtgcg aagtcggcgc gctcgctgct
     2341 cttcggcctc gtgctcgtgt cgggcgcgct atcggccttt ctcgtcaacg acacggtgtg
     2401 cgtcgtgctc acgccgctcg tcgtggcagt cgtcgtcgaa gcgcggcttc caccgctgcc
     2461 gtatctgctc gcgcttgcat cggctgccaa cgtcggcggt gtcgtcagct tctcgggcaa
     2521 tccgcagaac atgatcatcg gcgcacacgc gcacgagacg atcagcttcg cgcagtactt
     2581 cgtgctcgtg cttcccgtcg gcgcgctctg tctcgtcgtc aacgctgcgc tcatcgcgtg
     2641 gatgttccgc cgcgagctgc ccgcaggccc actcgccgaa cgctcgccgc cgcgcccagc
     2701 cgtcgatcgc gtgctcgcca ccaaaggcgt cggcgcgctc gcgctcttcg cgatcctggc
     2761 gctcgcaggc gtcagcctcg ccggtgcagc catgtgcgcc gcggccgtct tgatgctcgt
     2821 cgcgcgaacc ggtgcacgtc aggtgttcac gcgtgtcgat tggacgctgc tcgcattctt
     2881 cggcggtcta ttcgtcgtcg tcgcgggcgt cgcgcacgca ggcgtgctga acaagatctt
     2941 cgacgcggcg gcgcccgtga tcgcgcgcgg tgatgccacc ggcgatctgc tgttcgtgct
     3001 tttcgtcgtg atcgcgtcca acgtcgtttc gaacgtgccg ctcgtgctcg tcgcggtgag
     3061 ctggatcccg cacatgcccg atccgacgtg gggctacatc atgctcgccg tcgcatcgac
     3121 gctcgcaggt aacctcacgc tgttcggctc cgtcgctaac atcatcgtca tggagtccgc
     3181 cggcccgcgc ggcgaagtaa gcttctggcg cttcctgcgc tacggctcgg tgctcacgat
     3241 cgtcgacctg gtgctcgcgt tcggcctgct attcctcgag cgacgcgctg gaatcggcga
     3301 tttactcggc ctgtgaacgg ccggttacgc ggcgacgtga atgcgcggat tttcgccgct
     3361 cgcgcacggc acgcccgttg ctgaggagcg cggcgcatgc gcgtttgggt cgccatgatc
     3421 gtgctcgtgt tgtgcaacgt agcggccgcg gagccgcgag ccagcgtcgg ctttcgcgcg
     3481 acgtggaaca ccgacgcctc gccaccgccg tggctcatga cgggcgggca atggggcttg
     3541 cgccttcgcg ggacgacgtg gctcatgctc gacggcgatg gcatgcgcgg cgagaagctc
     3601 gagcacgtcg atggctcggc gagtcagcgc acattcttgg tgctgggtct cgggctgacg
     3661 accgacgtca tcgcgcaggg ccgcaacgcg ctgcgcgtgc tcggcggtgg aggcgtcgtc
     3721 gtcgagctcg cgcacgagcg catgctcgga acgtgggaca ccgccgatgt cgcgacgcgc
     3781 ccgcttggtt acgtcggcgc cgcgttcgag catcgcttcc cgcaattctg ggtggagaag
     3841 cgcaagaaat ggatgggcgg tggcgagatg ggcgtcgaag tccgcgcggt gcggctcttc
     3901 ggcgacggcg cgatgacgcc tgcgccgggc atcgccgcca catcgatgca ggcatcgctg
     3961 cagatgagcg cgtacttcta gcgcttgaac atgaagatgc cgtcgcggtc gagctccgtg
     4021 acgtacacgc cttcggtatc cgcgcgcagt gacggcgcct tgagcccgct cgcgatcgtc
     4081 tcgctcttcg cggcgcccgg cgcgagatgc acgagccgct tgtcctcgcc cgccttgccc
     4141 tcgaaccagt acgcgtggcc atcactcgtg acgacgaccg gctcctgatc gacatcgtga
     4201 ccgaccacct cgatcgcgcc gccggtcttc ttgcgacgac gaacttcgcc cgtgccctcc
     4261 gcgtaccaat agagcgagtt gccatcgatc gcgagatcga cgatgccctt ctgaccggtc
     4321 acgaccgtgc gcgctttgct gcctggcgtc ttgccgagct tgctgatcgt gcccgctgcc
     4381 cacgcgccga tccacacggc gtcgtcgtcg acggcgacca tgaactgctc cttcggacgc
     4441 gtcgcatgaa tcttctcggg cgtaccgcca ctgcgtagaa cgcgaaagag ccccggattc
     4501 tcggtgccct cgaagcccgg cacgacgaag taggcccact tgccatcgat cgcgctggtg
     4561 atgcagccct cgagaccgcc ggcgatcacc tgactgccgt tgtcggcacc ggcggtgatg
     4621 cgcatgagct gaccgtcgcc atcgcagaac acgacgccct gcgcatccgc gcgcatgtcg
     4681 aatgcactgc cgcaatcgcc gacattcacg gccgcgccgc cgctcttcga ctgcgcgtac
     4741 atcgtgccgc gctcgcagta atagaggacg ccgggcgcgc tcgcgaacca ctgcgtctgc
     4801 acatcgctga tctgcacgct gcccgcgatg cgcggtggcg gaggcggctc gtcaggcgcc
     4861 ggcgttggcg tcgtcgttgg cgtcggcggc ggttgatccg gcgtcgcggg cggctgctcg
     4921 tcaggcggtg gcgtgttcac cacgacggtc ggcggcttcg tcgcgtccgc tttctgcttc
     4981 ggcttcttgt tcagcgtgaa gcgaagtgcg agcccgagcg cgaccacacc gacgacgacc
     5041 aagaagaatt tcttcacgcc atgtgatgac accggccacg cgccgctgcc acgcaaaatc
     5101 cggcgcaacc gcgaatacct gcgcgtgtca gcgcgttgga gcttcggcgt tccaggcaca
     5161 ccgccagcgc acggcgccgt cgtgaatgcg cgcggcctcc gccttcccga gtgcggcaaa
     5221 atccggcgcc ccgacgagca gcgcgccgat tcccgacagc ccaggctcgt gcccgacgag
     5281 cacgacattc gcatccgccg ccagcgcctt caccatcgcg acgacgctgc gcagctcccc
     5341 atccggcgcc agcgccggca caacctcgac gctgatcttc gacgccaacc ccgccgctac
     5401 cagctccgcc gtctgcaccg cgcgcacgag cggcgatgtc cagatgtgcg tcggcgtgca
     5461 gtcgtgccag cgcagccgat cgccgagcgc gaccgcctgc tcgcgcccat gcgccgtcaa
     5521 atgccgcatc ggatcgcgca gctcgagcgt ctcgtcgatc gcctccgcgt ggcggatcaa
     5581 gaacacgtcc acggttcgag cgtaccatcg gacgcatgaa gctctacggc acgacgacat
     5641 ccccattcgt tcgccgcgtg cgcgtggtcg ccgccgaagt cgccgagccg gtcgagctga
     5701 tcaacaccgc acccgacgag ggccaagccg agctccgtcg catctcgccg atccgcaaag
     5761 tcccggtcgc cgtcgtcgat gcgacggtcg cgggtggcct cacgctcttc gactcgcgcg
     5821 tgatcatcga gtggctcacg acgacgcacg gctgggccga cctgacgccg acgcgcggcc
     5881 gctggcacga gcagaacatc atcaatgcga tcgatgccgc gctcgatgcg gtaatccagc
     5941 tgttctactt gcggcgcgat ggggtggcgg tggatggctc gccctacgcg gagaggcagc
     6001 tcgcacgcgc cgacgctatc ttcgcgtggc tcggcacgaa gctcgtcgac ggtcgcagct
     6061 tcagtggtgg cttcggctta ccggagctcg cgctgatctc ggcgctcgat tggatggact
     6121 tccgcaagac gtacgagacg tcgacgatcg gcgcggtcga ggcggtgcgc gaagcgtggc
     6181 gcgataggcc gtcgctcgcg tcgacgatgc cgcacgcatg agttacttca cgaaggacat
     6241 catgccagcg accctccacg tgatcttcgg gccctgcggc gccggaaaaa ccacctacgc
     6301 gcacgcgctc gcccgccgcg aaggtgcggt gcccttcgtc ctcgacgact ggggcgcgcg
     6361 cctcttcggc cccgacgtcg aggggccgat cgatttccga tggatgctgg agcgcctcgg
     6421 gcgctgcgag gcgcagatct ggttgaccgc cgccgacgtg ctggcggtcg ggactcccgt
     6481 ggtcctcgac atcggcgcga tgcgccgcgc ccagcgggaa cgcatccgcc agatcgcgaa
     6541 ggagaagggc ctgtcggtgc agtggcactt catcgacgcc ccgcaggagg tccgccgcgc
     6601 ccgcgtcgtc gaccgcaaca ccgccaaggg cgaaaccttc gtccgggaag tgccgccgga
     6661 gatgttc
//
LOCUS       DATLBZ010000093         8315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3804588, whole genome shotgun sequence.
ACCESSION   DATLBZ010000093 DATLBZ010000000
VERSION     DATLBZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804588"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..818
                     /locus_tag="VMZ53_04970"
     CDS             168..818
                     /locus_tag="VMZ53_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS27832.1"
                     /translation="MLAAKGKRTEFERTALVHLDAMYGAAYRLTRNPRDAEDLVQDSL
                     VRAYRFWDSFQQDSNCKAWLLRIVTNTFINEYQRRKRSREVLDAATSEQAATDGVLVH
                     ADANDKVSPEKAMLASSVSDDVQRALEQLPDDFRTAVILCDMQGLSYKEIADIMECPV
                     GTVMSRLFRGRKLLAQSLRSFAEQEGYVKKPQSVGTDKPLDLEKYRAEKKAREGSQ"
     gene            815..1672
                     /locus_tag="VMZ53_04975"
     CDS             815..1672
                     /locus_tag="VMZ53_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HUS27833.1"
                     /translation="MSLCKSIDTLSMAYLDDELAAEERHELEAHLTECSQCRSHVDQE
                     RADRSIVRKALAVGPAPDLLRARIGKALDAEDRAEQKAQRRRWYQYMLPGSAMVAAAA
                     AIALFVGVQNDTAPTGRSKAPLAASARPLTGGKVTSSVTNVTRRSLPLEVQGASTGPW
                     LRENFAPQIEPPKFANTDNDVIGARLLPGGIDGHDAALVAYNVTLHGKPFVLTVLFVR
                     DIGPSEWNEGDSVKVNNRMMHVLRAEDGNQMVSYVDNRGIGYIFNAPEISTEDLVSLV
                     GRTELVGDQ"
     gene            1738..5259
                     /locus_tag="VMZ53_04980"
     CDS             1738..5259
                     /locus_tag="VMZ53_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS27834.1"
                     /translation="MNRRVLLVDADPDFQGKLTSQLARYRVVLMTEPETDRALALAQA
                     DTPDLVIIGVEEPEKLGFKAFQKLRKALPQKMPIMLVTSSVPPEAFAKHKTMKVRANE
                     YMDKRGLGNEEMLAKIDNLIGLGDLQEDDVSVPVDDDIPMEIAEGDVVLDEQMGDDDD
                     AVSSFEHEMATVGPGGGVKMADLMEAETDAAFAALLGGDDEPAPKKRGGLPGVAPVPE
                     PVPEPVPEPVPEPLAEVDSGIPEPVPHKIPDDEVEEAAPSKAQWDMDSVPAPVHDGGR
                     PSDMYSPVDEHDDDFGNEEHVHVGAPPPSPNANADADADAAPEPILHAESDRRPVREP
                     SEPMERARETDEPEREPSERVAAAEAGASEHQASDVESDVAESDVSEEEVSSGLGNDS
                     LSAIPVMDDELVSLDDDMVEVEDEPVAPVEVEEPAAAAPVDMESTVARAPIITDAARR
                     PDKKTNAPVVDLGLDQIADTADAERDQSGVYDRKALRKIGELERQIAQLKTEVERARA
                     QADAAASGESKQSQFLKLREDKLAKEKELKDAKKTVEQQAKDLAEALEKLRQATHAKA
                     QLEQKNTELEERLLDDSGKAKDFGAKVQSLTAQVNQLETDLDAKTRAATAAETALAQA
                     EKDLATERATRAASASEAERTLRMEQERTIARHKGELAALRAELETVQDQALTRQREE
                     LEAAHAARLEESIEELRRANATEHSDAVEEIEKKHNQELVNLKAEQAGELSRTKNELN
                     SKIQELEVALSEAKSGGAVAIAAAQQEHQTTIEQMRQTYEAQLDSLRDEHAEALERDA
                     ESHGQALAQLKAQLDRATAEHGVKLAAARKEAEDVSAQAAADKATLEEQHDSAVTALR
                     QQHDSALAALRQQHDSTLADVRQQHESALAALRQQLDAERAQAADAAKQAADAHNAAL
                     AELQQTLEDERAQAAEAARQAAEAHRNALGEQQQKLEAQIAELHDTAQREVAEAKNQT
                     HAARKAAEEAAARATAEREELQQSHASQIGELEAKHERAIAVANGEVVKAKAVAEAER
                     QKEVAALKSEHERALQQMHSKHSAEISEMSAERDELKKGLSGARDSLKRSEGELASAV
                     QTIADRNADLRAHQAAIAERDQRIADLRKEIESLEQENASYQDQVLRAYQKIKSDEAM
                     VARARKAMAIALTVLDDQGNPTDKPSS"
     gene            5991..6833
                     /locus_tag="VMZ53_04985"
     CDS             5991..6833
                     /locus_tag="VMZ53_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUS27835.1"
                     /translation="MLRLVLAILCVSTGAPWARWAAPAPPLAIGAMRTGFASVLLFAV
                     GWRDIGKLWSLPARDKRLVIASGVLLGVHFGSWIASLSFTSTAASVALVATNPMFAAM
                     FGTLLGDRVTRREVMGIAIAAVGCAVLAGGDWQSGGDALIGDVLALIGAASAAAYLVV
                     GRRLRGSVPLFPYLGAVNAIAAVGLGAALVVSGADIAAISGHSVIACAGAAVVASLVG
                     HSLLNAAVRVTPTHLVALAILGEPVAASLTTWAAFGEQPPIHAALGAAIVIAGIAVGF
                     VRKR"
     gene            6843..8204
                     /locus_tag="VMZ53_04990"
     CDS             6843..8204
                     /locus_tag="VMZ53_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27836.1"
                     /translation="MRRLLVVMACLAGCGRFGFGTSADDVGGDDARDAAPDGFVPTGP
                     VGPRWVVAFTNDNSSSVVGRNGEVQILSRFVTTFQVDQGVSLTGNGFISTAFLRFDAA
                     GALTSSTVLDSDGFCDMRSAVLDGDATYLAGFTQGVTSMPAYGACSIVTNRQDPIAIK
                     IDQQGNQSLVAHWIAAGGNAQGWNVEVMPDHTLTIDGIYSVSLQIGPYTMPTGITDPS
                     VFVARTTSDVNTATWAKGFTAGVAIYPGPIASSGDDICIIGSHAGNVSVFGRALPYVG
                     GEDSWIARVDGGGTERFVRAIGSTGNEASYGGGAIIHALSDGGCLASIVAQGDVALDT
                     GMSLLADGAGLVVQLAADGKVTWARRFPSQPYLANVNGRWIAAYTTTNDVAVVEIDVQ
                     GGADVPLGVIEGAGVQDAVDIAAVGPDAVAVTVNTRGVLTFGATSFDSGATTLRAVAV
                     LGI"
BASE COUNT         1558 a         2717 c         2842 g         1198 t
ORIGIN      
        1 cacggacaag ggcacggtcc cgcatcctgc ggtggcggaa gctcttggcg tgcagtttgc
       61 gccgttcaag gcgtagcggc ccgacgattt ccctgccaga acggggactt gccttcctcg
      121 cacgagccgg gaacaatgtg cgtggggagg caggtagaag tccctgaatg ctggcggcga
      181 aaggcaaacg caccgagttc gagcggaccg cgctcgtcca cctcgatgcg atgtatggcg
      241 cggcctatcg gctgacgcgc aacccgcgcg acgccgagga tctcgtgcag gactcgctcg
      301 tccgcgcgta tcggttctgg gactcgttcc agcaggattc gaactgcaag gcgtggctgc
      361 tccgcatcgt cacgaacacg ttcatcaacg agtatcagcg gcgcaagcgg tcacgcgaag
      421 tgctcgacgc ggcaacgtcg gagcaggcgg cgaccgatgg cgtgctcgtg catgccgacg
      481 cgaacgacaa ggtgtcgccc gagaaggcga tgcttgcgtc gagcgtgtcg gacgacgtgc
      541 aacgcgcact cgagcagctg cccgacgatt tcaggactgc cgtgatcctg tgcgacatgc
      601 agggcctctc ctacaaagag atcgccgaca tcatggagtg tcccgtggga acggtgatga
      661 gccggctctt ccgcgggcgt aagctattgg cgcagtcgct gcgatcgttt gccgagcaag
      721 aaggttatgt gaagaaaccg cagtcggtcg gaacagacaa accgctcgat ctcgagaagt
      781 accgcgccga gaagaaggcg cgtgagggtt cgcaatgagt ctttgtaaga gcatcgacac
      841 tctatcgatg gcgtatctcg acgacgagct cgctgccgag gagcgccatg agctcgaggc
      901 gcacctgacg gagtgctcgc agtgtcgtag tcacgtcgac caagaacgcg ctgatcgctc
      961 gatcgtgcgc aaagcgctcg ccgtcgggcc cgcgccggat ttgctgcgcg cgcggatcgg
     1021 caaggcactc gacgccgaag atcgcgcgga gcaaaaggcg cagcgtcgcc gctggtatca
     1081 gtacatgctg ccgggctcgg cgatggtggc ggctgcggcc gcgatcgcgc tgttcgtcgg
     1141 cgtgcagaac gacaccgctc caacgggccg ctcgaaggcc ccgctcgctg caagcgcgcg
     1201 ccctctcacc ggcggcaagg tcacctcgtc cgtcacgaac gtcacgcggc gctcgctgcc
     1261 tctcgaggtg cagggcgcga gcacgggccc gtggctgcgc gagaacttcg ctccgcagat
     1321 cgaaccgccg aagttcgcca acaccgataa cgatgtcatc ggcgcgcgcc tcttgccggg
     1381 cggcatcgat ggccacgacg ccgcgctcgt cgcctacaac gtcacgctgc acggcaagcc
     1441 gttcgtgctg accgtcctgt tcgttcgcga catcggtccg agcgagtgga acgaaggcga
     1501 ctcggtcaag gtcaacaacc gcatgatgca cgtgctgcgc gccgaagacg gcaaccagat
     1561 ggtgtcgtac gtcgataacc gcggcatcgg gtacatcttc aacgcgccgg aaatctcgac
     1621 cgaggacctc gtgagcctcg ttggacgcac cgagctcgtt ggcgaccagt aggtccgctc
     1681 cgaaaacccc ccagcagcgc attaccgact caactcggat atgatcgcgc caggggcatg
     1741 aaccggcgcg ttcttcttgt cgatgctgat cccgactttc agggcaagct gaccagccag
     1801 ctcgctcggt atcgcgtcgt gctgatgacc gagcccgaga cggaccgcgc acttgcgctg
     1861 gctcaggccg acacgccgga cctcgtcatc atcggcgtcg aggagcccga gaagctcggc
     1921 ttcaaggcgt tccagaagct gcgcaaagcg ctgccgcaga agatgccgat catgctcgtg
     1981 acgtcgagcg tgccacccga ggcgttcgcg aagcacaaga cgatgaaggt gcgcgcgaac
     2041 gagtacatgg acaagcgcgg cctcggcaac gaggagatgc tcgcgaagat cgacaacttg
     2101 atcgggcttg gcgatctgca agaagacgac gtctcggtgc cggtcgacga cgacattccg
     2161 atggagatcg ccgaaggcga cgtcgtgctc gatgaacaga tgggcgacga cgacgacgcc
     2221 gtctcgagct tcgagcacga gatggcgacg gtcggccctg gaggcggcgt gaagatggcc
     2281 gacctcatgg aggccgagac ggatgctgca ttcgcggcgc tgctcggtgg tgacgatgag
     2341 cctgcgccga agaagcgcgg aggcttgccc ggtgtcgccc cggtgcccga gccggtgccc
     2401 gaaccggtgc ccgagcccgt gccggagccc ttggcggaag tcgattccgg catcccggag
     2461 ccggtcccgc acaagattcc cgacgacgag gtcgaggaag ccgcgccctc gaaggcgcag
     2521 tgggacatgg acagcgtccc cgcacccgtg cacgacggtg gccgcccgtc cgatatgtat
     2581 tcgcccgtcg acgagcacga cgatgacttc ggcaacgaag agcacgtgca tgtcggcgca
     2641 ccgccgccgt cgcccaacgc caacgccgat gccgatgccg atgcggcgcc cgagccgatc
     2701 ttgcacgccg agtccgaccg gaggcccgtg cgcgagccga gcgagccgat ggagcgtgcg
     2761 cgcgagacgg acgagcccga gcgtgagccg agcgagcgcg tcgcggctgc ggaagccggt
     2821 gcctccgagc atcaggcgtc ggatgtcgag tcggatgtcg ccgagtcgga tgtctccgaa
     2881 gaagaagtct cgtcgggcct cggcaacgat tcgctgtcgg cgattccagt catggacgac
     2941 gagctagtct ccctcgacga cgacatggtc gaagtcgaag acgagcccgt cgcgccggtc
     3001 gaggtagagg agcccgctgc cgcggcgccc gtcgacatgg aatcgaccgt cgcccgtgcg
     3061 ccgatcatca ccgacgccgc gcgccgtccc gacaagaaga cgaatgcgcc cgtcgtcgat
     3121 ctcggcctcg atcagatcgc cgacaccgcg gatgccgagc gcgatcaatc cggcgtctac
     3181 gatcgcaagg ctctgcgcaa gatcggcgag ctcgagcgcc agatcgcgca gctgaagacc
     3241 gaagtcgagc gcgctcgcgc acaagcggac gctgcggcgt cgggcgagag caaacagtcg
     3301 cagttcttga agctccgcga ggacaagctc gcgaaggaaa aagagctcaa ggacgccaag
     3361 aagaccgtcg agcagcaagc gaaggacctc gccgaagcgc tcgagaagct gcgccaagcg
     3421 acgcacgcaa aggcccagct cgagcagaag aacaccgagc tcgaggaacg cctgctcgac
     3481 gacagcggca aggcgaaaga cttcggcgcg aaggtccagt cgctgaccgc gcaggtcaac
     3541 cagctcgaga ccgacctcga cgcgaagacg cgcgccgcga cggccgccga gactgcgctc
     3601 gcacaagccg agaaagatct cgcgaccgag cgcgcaactc gcgccgcatc cgcatcggaa
     3661 gccgaacgca cgttgcgcat ggagcaagag cggacgatcg cgcggcacaa gggcgagcta
     3721 gccgcgttgc gtgcggagct cgagacggtg caagaccagg cgctcacgcg ccagcgcgaa
     3781 gagctcgagg ctgcgcacgc cgcgcggctc gaagaatcga tcgaggagct gcgtcgcgcg
     3841 aacgcgaccg agcactccga cgcggtcgag gagatcgaga agaagcacaa ccaagagctc
     3901 gtcaatctca aggccgagca ggccggcgag ctgtcgcgca ccaagaacga gctcaacagc
     3961 aagatccaag agctcgaggt cgcgctctcg gaagccaagt ccggcggcgc cgtcgcgatc
     4021 gcagccgcgc agcaggagca ccagaccacg atcgagcaga tgcgccaaac ctacgaggcg
     4081 cagctcgact cgctccgcga cgagcatgcc gaagcactcg agcgcgacgc cgaatcccac
     4141 ggccaagcgc tcgcgcagct caaggctcag ctcgatcgcg caaccgccga gcacggcgtg
     4201 aagctcgcgg ccgcgcgcaa agaagccgag gacgtctccg cacaagcggc cgccgacaaa
     4261 gccaccctcg aagagcagca cgactccgcg gtcacggccc tccgccagca gcacgactcc
     4321 gcgctcgccg ctcttcgcca gcagcacgac tccacgctcg ccgacgtccg ccagcagcac
     4381 gagtccgcgc tcgccgctct ccgccagcag ctcgacgcag aacgcgcgca agccgcggat
     4441 gcggcgaagc aagccgccga cgcacacaac gccgcgctcg ccgagctgca gcaaacgctc
     4501 gaagacgaac gcgcccaagc cgccgaggcc gcacgccaag ccgccgaagc ccaccgcaac
     4561 gcgctcggcg agcagcagca aaagctcgaa gcgcaaatcg cggagctgca cgacaccgcc
     4621 cagcgcgaag tcgcggaagc caagaaccaa acgcacgccg cacgcaaggc cgccgaagaa
     4681 gccgcagccc gtgcaacggc cgagcgtgaa gagctgcagc aatcacacgc ctcacagatc
     4741 ggcgagctag aagccaagca cgagcgcgcg atcgcggtcg caaacggcga ggtcgtcaaa
     4801 gcaaaggcgg tcgccgaagc cgaacgccaa aaggaggtcg ccgcgctcaa atccgagcac
     4861 gagcgggcac tccagcagat gcactccaag cacagcgcgg aaatcagcga gatgtccgcc
     4921 gagcgcgacg agctcaagaa gggcctctcc ggcgcgcgcg attcactcaa gcgctccgaa
     4981 ggcgagctcg cgagtgcagt ccaaacgatc gccgaccgca acgccgacct ccgcgcgcac
     5041 caagcggcga ttgccgagcg cgaccaacgc atcgccgacc tccgcaagga gatcgagtcg
     5101 ctcgagcagg aaaacgcgag ctaccaggac caagtgctgc gcgcctacca gaagatcaaa
     5161 tccgacgagg cgatggtcgc gcgcgcgcgc aaagcgatgg caatcgcgct cacggtgctc
     5221 gacgaccagg gcaacccgac cgacaagcca tcgtcgtaac gttcccgccg ccagttcgca
     5281 tcgccctccg cgacagcgtg catcgcgcta accgtccgcc acggcagttc gcatcgcgcg
     5341 cgcgcgcggc gggcgtcttc gcggcagggc cgtcgtcgcc gcgtcagcgc ggtggggcgg
     5401 ccagtgcgct ctgccgctag tcgcgggcga gggtgcgggg caagcgcacg tgcgagggga
     5461 tacgcgtcgc cgagaccctg ccgctacgcc gcccggggac gcgcgcgcgc gcgatgcgac
     5521 ctgccgatct aaccgcggag aagaaccgtg gccacaagcg cgatgcgatc gccaacggac
     5581 gatgaatgcg tgagatcttg ttgtctcgca cggcgcgtct ccgagggccc agcccggaga
     5641 gcgagcgtag gcgcgcgacg agacgcaggg ccccgccggc agaaccgcac aaggcctgcg
     5701 aagcaggccg ccgatcgtgc aacgatgaga acccgcgacg ggagcgccgt cacgcgagcg
     5761 ggtccgggcg gtcctcggag gtgcgccgtg cgcagcgctt tcaagccgcg agccggcaaa
     5821 ctcgacaacg acgccgcgcg cgagcgcgat gcgagctgcc taccgcggat gcgagtggcg
     5881 gactacggga cgctcacggc gatgcgaacg tccaacggcc gcaaagccaa acgcgcaacg
     5941 gcgcagaggc gggcgcaata gcggcgcagc gagcgtagag tcgaggcgca gtgctgcggc
     6001 ttgtcctcgc catcttgtgc gtctcgacgg gtgcaccgtg ggcgaggtgg gcggcccctg
     6061 ccccgccgtt agcgatcggc gcgatgcgca ccggctttgc gagcgtgttg ctgtttgcgg
     6121 tgggatggcg cgatatcggc aagctgtggt cgctgccggc gcgtgataag cggcttgtga
     6181 tcgcgtccgg cgtcctactc ggcgtgcact tcggcagctg gatcgcgtcg ctctcgttta
     6241 cgtcgacggc ggcgagcgtc gcgctcgtcg cgacgaaccc gatgtttgcg gccatgttcg
     6301 gcacgctgct cggcgatcgc gtcacgcgtc gcgaagtgat gggcatcgca attgccgccg
     6361 tggggtgcgc cgtcttggcg ggcggcgact ggcagtcggg tggcgatgcg ttgatcggcg
     6421 atgtgctcgc gctgatcggt gcggcaagcg cggcggcgta tctggtcgtc gggcggcgat
     6481 tgcgcggaag cgtgccgctc tttccgtatt tgggtgcggt gaatgcgatc gcggcggttg
     6541 ggttgggcgc ggcgctcgtc gtgagtgggg ccgacatcgc ggcgatatct gggcattcgg
     6601 tgatcgcgtg cgccggtgcg gcggtggtgg cgtcgttggt cgggcacagc ttgctcaatg
     6661 ccgcggtgcg ggtgacgccc acccatctcg tcgcgcttgc catcttgggc gagcccgttg
     6721 cggcatcgct cacgacgtgg gcggcgttcg gcgagcagcc accgattcat gcggcgcttg
     6781 gcgcggccat cgtcatcgcg ggaattgccg tcggtttcgt gagaaagcgc taagatcgaa
     6841 tcgtgcgacg gctgctggtt gtaatggcat gccttgccgg ttgcggacgg tttggattcg
     6901 gcacgtccgc cgacgatgtc ggcggcgacg atgcacggga tgcagcgccg gatggcttcg
     6961 tgccgacggg gcccgtggga ccgcgctggg tcgtcgcctt cacgaatgac aacagcagct
     7021 cggttgtcgg ccgcaatggc gaggttcaga tcctcagccg gttcgtcacg acgtttcagg
     7081 tggatcaagg cgtgagcctc accggcaacg gtttcatcag cacggcattc ctgcgcttcg
     7141 atgcggcggg cgcgctgacg tcgtcgacgg tcctcgactc cgacgggttc tgcgacatgc
     7201 gatcggccgt gcttgatggc gacgcgacgt acctcgctgg cttcacgcaa ggcgtgacgt
     7261 cgatgcctgc ttatggcgcg tgcagcatcg tgaccaatcg gcaggatcca atcgcgatca
     7321 agatcgatca gcagggcaat cagtcgctcg tcgcgcattg gatcgcggcc ggcgggaatg
     7381 cgcagggctg gaacgtcgag gtcatgcccg atcacacgct gacgatcgac ggcatctatt
     7441 cggtgtcgct ccagatcggt ccgtacacca tgccgaccgg catcaccgat ccgagcgttt
     7501 tcgtggcgcg cactacgagc gacgtcaaca cggcgacgtg ggcgaaggga ttcacggccg
     7561 gcgtcgcgat ctatccgggc ccgatcgcga gtagcggcga tgacatttgc atcatcggct
     7621 cgcatgccgg caacgtgagc gtgttcggtc gcgcgctgcc gtatgtcggt ggcgaggaca
     7681 gctggatcgc gcgagtggat ggcggcggca ccgagcgctt cgtgcgcgcg atcgggtcca
     7741 ccggcaacga ggcgtcgtac ggcggcggag cgatcatcca tgcactctcc gacggcggtt
     7801 gcctcgcgtc gatcgtcgcg cagggtgatg tcgcgctcga taccggcatg tcgctcctcg
     7861 ccgatggcgc tgggctcgtc gtgcagctgg ctgccgacgg caaggtcacg tgggcgcgtc
     7921 ggttcccgag ccagccgtat ctcgcgaacg tcaacggtcg ctggatcgct gcgtacacga
     7981 cgacgaatga cgtcgcggtc gtcgagatcg acgttcaggg cggtgccgac gttcccctcg
     8041 gcgtcatcga aggcgcgggt gtgcaagacg ccgtcgacat cgctgcggtc ggccccgacg
     8101 cggttgcagt cacggtgaac acgcggggag tgctgacatt cggggcgacg agcttcgatt
     8161 cgggcgcgac tacgctgcgt gccgtcgccg tgctcggcat ctagcggcta ctcgacaagc
     8221 cgcccgatga ccgattgtat ttgtgggtga ccgcggctgc cgctgattac ggtccgcggc
     8281 atgaagcgcc tcgcctacgt catactcagt gcctg
//
LOCUS       DATLBZ010000094         5346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3804824, whole genome shotgun sequence.
ACCESSION   DATLBZ010000094 DATLBZ010000000
VERSION     DATLBZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804824"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /gene="speA"
                     /locus_tag="VMZ53_04995"
     CDS             <1..1179
                     /gene="speA"
                     /locus_tag="VMZ53_04995"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HUS27837.1"
                     /translation="LHFHLGSQISAIKSVKNALREAGRLFCEMYKLGAKGLCYMDVGG
                     GLGVDYDGSQTNFASSMNYTVQEYANDVVYAIKEICDADQVPHPNIVSESGRAVAAHH
                     SVLVVNTLGVTEFNTHVPAQLSKPAPPLVTNMWDTYQAVSQKNLLESYHDAVEYKDQV
                     LQLFNLGHLSLEHRVLCENLFWAICEKVLMISRNQAHVPEELEGLEKALSDTYFCNFS
                     MFQSVPDAWAVDQLFPICPIHRLTEEPTRRATLADITCDSDGKIDSFIDLRDVKHVLE
                     LHPKHEGQDYYLGIFLVGAYQEILGDLHNLFGDTNTVHVQLSPDGDYDVKEVVAGDTI
                     SEVLQFVDYSPSDLLGRMRNNVEQALRKKLMTIEESRTLINRFRQGMIGYTYLDREPE
                     "
     gene            1194..1601
                     /locus_tag="VMZ53_05000"
     CDS             1194..1601
                     /locus_tag="VMZ53_05000"
                     /inference="COORDINATES: protein motif:HMM:NF020544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HUS27838.1"
                     /translation="MGACCYVRRVSTRQLDALVADRSKLERVLFPSTTETAVDDDVFI
                     DLDTVWPSLVEAVVALNPALALLDRGGADLGKLNGHPAVGFATADARRIAAALSLLTP
                     DNVPDPVREKFEMLKDFVTETAKAGAGMIVFWR"
     gene            1626..2174
                     /locus_tag="VMZ53_05005"
     CDS             1626..2174
                     /locus_tag="VMZ53_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27839.1"
                     /translation="MANRSYLYTCDQVPTDESTDAPRGLSELNYDIPLIHMLMAANNP
                     RRVQSAIWSEHDIAILADREHAIERALAFADKVGAGTLDDRAAWDSDLAAMREFFAKV
                     PPSKYVLLECGEIWEMQCDSLDAIKAAADKLVTDCQATGARVDRALAGKEDTWLDEVR
                     TEWQRHFGASWSEHLYFSFGDQ"
     gene            2171..2629
                     /locus_tag="VMZ53_05010"
     CDS             2171..2629
                     /locus_tag="VMZ53_05010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HUS27840.1"
                     /translation="MIALRAATATDLDDILPRTRALNDHEGITIATPQLRAALEKLLG
                     DRSLGGVWVIEDAAQPATTATTIGYAIVTFGFDLEFGGRDAWLTELWVDESARGRGAG
                     TTALALLEPELRALDVKAYHLQVREQNPALRLYERAGFARVPRLILTRRL"
     gene            complement(2686..3366)
                     /locus_tag="VMZ53_05015"
     CDS             complement(2686..3366)
                     /locus_tag="VMZ53_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27841.1"
                     /translation="MTTPIAPDIWHVELEPLKFPGGVRMPLSSTVIKLPTGGVVLYSP
                     GKLDDTQIGAINALGEVTHIIAPSLFHHLYAGKAHELWPKATLHGAPGLAAKRTDLKF
                     GAELGRSFDDALDFVHIGGAPSFNEVVMFHRPSGTLACADFLFNVTKPANLRTRFALS
                     ITGVHGRTLKQSRLWKWSTKDKAAARASLDKILEWPIQHIAPVHGDPVDIDRDHLKPL
                     LRGPFKIA"
     gene            complement(3363..3878)
                     /locus_tag="VMZ53_05020"
     CDS             complement(3363..3878)
                     /locus_tag="VMZ53_05020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS27842.1"
                     /translation="MPIRAARADDFATLAAITNHYITTSSIHFAYEPVTDADLRAMWE
                     RSRDRHPWLVTQDGEAGEVLGYAKSGVWRERAAYNWTCEVGLYIADAARGRGLGRALY
                     EALLAECARRGFRSAVAGITLPNDPSIALHEKCGFARVGTFADAGWKHGAWHAVEFWQ
                     KRFATTLEGPP"
     gene            3903..4274
                     /locus_tag="VMZ53_05025"
     CDS             3903..4274
                     /locus_tag="VMZ53_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27843.1"
                     /translation="MLKRICLLVLVVAGCEKSPGPSCEQISDHMLDVTKQAMPGHDPA
                     YLGDRKAMVAQCEKRNPTREMRNCLMKAKTLAELGECQAKPGDKKPDPQPSRPLPTTA
                     PEAPAPAQPVAPAAVPSGSGG"
     gene            4329..>5346
                     /locus_tag="VMZ53_05030"
     CDS             4329..>5346
                     /locus_tag="VMZ53_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUS27844.1"
                     /translation="MHDAVIHVPDPINEPIYPYAPGSPEKARLKAALADIEREVVEIP
                     CVVGGQYVKTGKIREVVMPHRHKHVVARFHAAEPDTAERAIRAALEAKKEWAAMRWEA
                     RAACLLRAAELIAGPWRMKINASTMHGQSKTCHQAEIDAACEMIDFFRFNAAFAQQLY
                     QMQPKSAPGMWNMSELRPLEGFVFALTPFNFTAIGGNLPTAPAVMGNTVVWKPAESQL
                     LSAWRTFQVFEEAGFPPGVINFIPGLGHQHTNILLDHPDFAGLHFTGSTDVFNMMWKR
                     VGDNLGKYKSYPRLVGETGGKDFILAHPSADPKALATAIIRGGYEYQGQKCSAASRVY
                     VAQSV"
BASE COUNT          962 a         1746 c         1744 g          894 t
ORIGIN      
        1 ctccacttcc acctcggcag ccagatcagc gcgatcaaga gcgtgaagaa cgcgctgcgc
       61 gaagcaggcc gcctgttctg cgagatgtac aagctcggcg cgaagggcct ctgctacatg
      121 gacgtcggcg gcggtctcgg cgtcgactac gacggctcgc agacgaactt cgcgtcgtcg
      181 atgaactaca cggtgcagga gtacgcgaac gacgtcgtct acgcgatcaa agagatttgc
      241 gacgccgacc aagttccgca tccgaacatc gtcagcgagt caggtcgcgc agtcgccgcg
      301 caccactccg tcctcgtcgt gaacacgctc ggcgtgaccg agttcaacac gcacgttccc
      361 gcgcagctgt cgaagccggc accgccgctc gtcacgaaca tgtgggacac gtaccaagcg
      421 gtcagccaga agaatttgct cgagagctat cacgacgccg tcgagtacaa ggaccaagtc
      481 ctccagctct tcaacctcgg ccacctctcg ctcgagcacc gcgtgctctg tgaaaacctc
      541 ttctgggcga tttgcgagaa ggtgctgatg atctcgcgca accaggcaca cgtccccgag
      601 gagctcgaag gtctcgagaa ggcgctgtcg gacacgtact tctgcaactt ctcgatgttc
      661 cagtcggtgc ccgatgcgtg ggccgtcgac caactgttcc cgatctgccc gatccatcgc
      721 ctcaccgaag agccgacgcg ccgcgcgacg ctcgccgaca tcacgtgtga ctccgacggc
      781 aagatcgatt cattcatcga tctgcgcgac gtcaagcacg tgctcgagct gcatcccaag
      841 cacgagggcc aggactacta cctcggcatc ttcctcgtcg gcgcgtacca agaaatcctc
      901 ggcgacctcc acaacctgtt cggcgacacg aacacggtgc acgtccaact ctcgcccgat
      961 ggcgattacg acgtgaagga agtcgtcgcg ggcgacacga tcagcgaagt gctccaattc
     1021 gtcgactact cgccgagcga cctcctcggc cgcatgcgca acaacgtcga acaggcgctg
     1081 cgcaagaagc tgatgacgat cgaagagagc cgcacgctca tcaatcgctt ccgccagggc
     1141 atgatcgggt acacgtacct cgatcgcgaa cccgagtaag ttaagctcac gccatggggg
     1201 cgtgttgtta cgtgcggagg gtgtcgacga ggcagctcga tgcgctcgtc gccgatcgct
     1261 cgaagctcga gcgcgtgctg tttccgagca cgacggagac cgccgtcgac gacgatgtct
     1321 tcatcgactt ggacacggtg tggccgtccc tcgtggaggc tgtcgtcgcg ctcaatccgg
     1381 cgctcgcgct gctcgaccgc ggcggcgcgg acctcggtaa gctcaacggc catcccgccg
     1441 tcggcttcgc gacggcagac gcgcgtcgta tcgcggcggc gctctcgctg ttgacgccgg
     1501 acaacgtgcc cgacccggtc cgcgagaagt tcgagatgct gaaagacttc gtgaccgaga
     1561 cggcgaaggc tggcgccggc atgatcgtgt tctggcgatg acgcccttgc ggtagcttcg
     1621 cgagcatggc gaacagatcg tatctgtaca cctgcgatca ggtgccgacg gacgagagca
     1681 ccgatgcgcc gcgaggactg tcggagctca actacgacat cccgctcatt cacatgctca
     1741 tggcggcgaa taacccgcgt cgcgtgcagt ccgcgatctg gagcgagcac gacattgcga
     1801 tcctggcgga tcgagagcac gcgatcgagc gcgcgctcgc gttcgccgac aaagtgggcg
     1861 cgggcaccct cgatgatcgc gccgcttggg atagcgacct cgcagcgatg cgcgaattct
     1921 tcgcgaaggt gccgccgagc aaatacgtgc tcctcgagtg cggagagatc tgggagatgc
     1981 agtgcgatag cctcgacgcc atcaaggctg ctgcggacaa gctcgtgacg gactgccaag
     2041 cgacgggtgc ccgtgtcgac cgcgcgctcg ccggcaagga agacacgtgg ctcgatgagg
     2101 ttcgcaccga gtggcagcgg cacttcggcg cgtcgtggag cgagcacctc tacttcagct
     2161 tcggggacca atgattgctc tgcgcgctgc tacggctacc gacctcgacg acatcctgcc
     2221 gcgcacgcgc gcgctcaacg accacgaggg catcacgatc gcaacgccgc aactgcgcgc
     2281 agcgctcgag aagctgctcg gagatcgatc gctcggcggg gtgtgggtca tcgaggatgc
     2341 ggcgcagccc gccacgaccg cgacgacgat tggctacgcg atcgtcacgt tcggcttcga
     2401 tctcgagttc ggcgggcgcg acgcctggct gacggagcta tgggtcgacg agagtgcgcg
     2461 cggtcgtgga gcaggcacga cggcgttggc gctgctcgag ccggagctgc gtgcgctcga
     2521 tgtaaaggcg tatcacctgc aggtccgcga gcagaatcct gcgctgcgcc tctacgagcg
     2581 cgccggcttt gcgcgtgtgc cccgattgat tctcacgcgc cggctctgat acgcgccggt
     2641 ctcgagcgcg tgccgcgcac ctcgtgcccg cgtcggctat gaccgctacg cgatcttgaa
     2701 cgggccgcgc agcagcggct ttaggtgatc gcgatcgata tcgaccgggt cgccgtgcac
     2761 cggcgcgatg tgctggatgg gccactcgag aatcttgtcg agcgatgccc gcgcagcggc
     2821 cttgtccttt gtcgaccact tccagagccg gctctgcttg agcgtgcggc cgtgcacgcc
     2881 ggtgatcgag agggcgaagc gcgtgcgaag gttcgcaggc ttcgtgacgt tgaacaggaa
     2941 gtccgcgcag gcgagcgtgc cgctcggccg gtggaacatc accacctcgt tgaacgacgg
     3001 cgcgccgccg atgtgcacga agtcgagcgc gtcatcgaac gagcggccga gctcggcacc
     3061 gaacttcagg tccgtccgct tcgcggcgag acccggtgca ccgtgcagcg tcgccttggg
     3121 ccagagctcg tgcgctttgc ccgcgtacag gtgatggaat aggcttggcg caatgatgtg
     3181 cgtgacctcg ccgagcgcgt tgatcgcgcc gatctgcgtg tcatcgagct tgcccggcga
     3241 gtagaggaca acgccgccgg tcggcagctt gatcaccgtc gacgagagcg gcatccgtac
     3301 accgcccgga aatttcagcg gctcgagctc tacatgccag atatcgggcg cgatcggcgt
     3361 tgtcatggcg gtccctccag tgtggtcgcg aaacgtttct gccaaaactc gacggcgtgc
     3421 cacgcgccgt gcttccagcc tgcatcggcg aacgtgccga cgcgtgcgaa gccgcacttc
     3481 tcgtggagcg cgatgctcgg atcgttcggc agcgtgatgc cggcgaccgc cgagcgaaag
     3541 ccgcgacgcg cgcactctgc gagcagtgcc tcgtacagcg cgcgaccgag gccgcgaccg
     3601 cgcgcagcgt ccgcgatgta gaggccgacc tcgcacgtcc agttgtacgc ggcgcgctcg
     3661 cgccacacgc ccgacttcgc gtagccgagg acctcgccgg cttcgccgtc ttgcgtgacg
     3721 agccacggat ggcgatcgcg agagcgctcc cacatcgcgc ggaggtcggc gtcggtgacc
     3781 ggctcgtacg cgaagtggat cgaggatgtc gtgatgtagt ggttcgtgat cgccgcgagc
     3841 gtcgcgaagt cgtcagcgcg agctgcgcga atcggcatgg ctggcatgct accgtcgaag
     3901 gcgtgctgaa gcgcatctgc ttgctcgtcc tcgttgtcgc aggttgcgag aagagccctg
     3961 gcccgagctg tgaacagatt tcggaccaca tgctcgacgt cacgaagcag gcgatgcccg
     4021 gccacgaccc ggcgtacctc ggcgatcgca aggcgatggt cgcgcagtgt gagaagcgca
     4081 atccgacgcg cgaaatgcgc aattgcctga tgaaggcgaa aacgctggcc gagctcggcg
     4141 agtgccaggc gaaacccggc gacaagaagc cggatcctca gccgagtcgc ccgcttccga
     4201 cgacggcgcc tgaggctcca gcgcccgcgc agccggtcgc gccagccgcc gttccgtcgg
     4261 gttccggcgg ttagccgctg tccatagcgt cggctgatcg tttgggcagg gcgtggtaca
     4321 cgtgcgccat gcacgacgcg gtcattcacg ttcctgaccc gatcaacgag ccgatctatc
     4381 cgtacgcgcc gggcagcccc gagaaggcgc gcctcaaggc agcactcgcg gacatcgagc
     4441 gcgaggtcgt cgagattccg tgcgtcgtcg gcggtcagta cgtgaagacc ggcaagatcc
     4501 gcgaggtcgt gatgccgcac cgccacaagc acgtggtcgc gcggtttcac gcagcggagc
     4561 ccgacaccgc ggagcgtgcg attcgcgctg cgctcgaggc gaagaaagaa tgggcggcga
     4621 tgcggtggga agcgcgcgcg gcgtgcctgc ttcgtgcggc cgagctcatc gccggtccgt
     4681 ggcgcatgaa gatcaacgcg tcgacgatgc acgggcagag caagacctgc catcaggccg
     4741 agatcgacgc cgcgtgcgag atgatcgact tcttccggtt caacgccgcg ttcgcgcagc
     4801 agctctatca gatgcagcca aagagcgcgc ccggcatgtg gaacatgagc gagctgcgtc
     4861 cgctcgaagg cttcgtcttc gcgctcacgc cgttcaactt caccgcgatt ggcggcaacc
     4921 tcccgacggc gcctgcggtg atgggtaaca ccgtcgtgtg gaagccggcc gagagccagc
     4981 tgctctctgc gtggcgcacg ttccaggtct tcgaggaagc gggcttcccg ccgggcgtca
     5041 tcaacttcat cccgggcctc ggtcatcagc acacgaatat cctgctcgac cacccggact
     5101 tcgcgggcct tcacttcacc ggctcgaccg acgtcttcaa catgatgtgg aagcgcgtcg
     5161 gcgataacct cgggaagtac aagagctacc cgcgtctcgt cggtgagacg ggcggcaaag
     5221 acttcatcct cgcgcatccg tcggcggatc cgaaggccct cgcgaccgcg atcattcgcg
     5281 gcggctacga gtaccaaggc cagaagtgct cggccgcgtc gcgcgtctac gtggcgcaga
     5341 gcgtgt
//
LOCUS       DATLBZ010000095         3643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8237271, whole genome shotgun sequence.
ACCESSION   DATLBZ010000095 DATLBZ010000000
VERSION     DATLBZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8237271"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VMZ53_05035"
     CDS             complement(<1..1409)
                     /locus_tag="VMZ53_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27845.1"
                     /translation="MGNPENSPPVRPTTVAEDARWDPKDPGFEWVSGVLDSDGRRHGL
                     YRSWTRDGVLHGECTYDHGKVHGKNINFHPDGTVASEAEWQHGVIMNSAFYRSEAPSP
                     EPFAQAAPNVWSAKYYTRDGKTNYTIRYFLRDGTECGPDGNALPARPKSVSSDARWFP
                     DMDRWVDGAIERGTNKQVGRWRWWSRDGILRHEEVRDASGEATMIAEYEADGSLQKKT
                     TRTADGEERDYYFDDGSLSTRYREDAKGRQTYKGSWLRDGTLHEERVRTYDGDAIASV
                     TERARGGVLELEARREGPALACVLYHGDGKAIAATGGHNGEKLTGTWKIFDASGALRR
                     EIDVTPLELRFGVTGEGLEWKLGEALFKIDEPALPLPDQLIGVDAEPWADTHGCYDDC
                     VDEFPAYLRGLASPDPLVRRYCLGSIGTEIEHQGSVYPATARVVPYLARLLSHANVDR
                     AGLLTTIQSAAENAAPYVD"
     gene            complement(1472..2185)
                     /locus_tag="VMZ53_05040"
     CDS             complement(1472..2185)
                     /locus_tag="VMZ53_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HUS27846.1"
                     /translation="MIGLVLSCEHASWTLPPGVDLGVPVEALQSQAGWDHGAFDIASR
                     LSEAVGLPVHAGAFSRMFVDLNRGPDHADVIPAICYGEPVPGNVHLSAGDKAARIALF
                     HAPYWDAIRRDVSARLIDCGAVLHFSSHSFAPELDPSNRTYDCGVLYDPAHEFEAELA
                     ERLMFMLRGAGLSVRANQPYSGVGPAVCTSLRQELAGKKYAGIELETSHAVTRSHGGC
                     ARVAAAVVPFLESLQLPPT"
     gene            complement(2254..3099)
                     /locus_tag="VMZ53_05045"
     CDS             complement(2254..3099)
                     /locus_tag="VMZ53_05045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUS27847.1"
                     /translation="MRTAVLLLAIAACSSGSSTPPPSTTTTTQATGQNTPAMTGPLSE
                     AEFKALHEPPATPPQHLKGEVIDLAGTKAYLSLPQGKGPFPAIVVIHEWWGLNGNIKH
                     WADRLAATGWAALAVDLYGGTVATTPDEAMAAMKAVDQEKAKGTINAAFEFLAKDSRI
                     AAKKKAVIGWCFGGGWSLQTALAHPELDGAIVYYGQLETDPAKLGAIKGQLLGVFGNK
                     DKGIPPAKVDELEAALKTAGVTAQIYRYDAEHGFANPSNPKYDETSSADAWTHVLAFL
                     EGLKK"
     gene            complement(3119..>3643)
                     /locus_tag="VMZ53_05050"
     CDS             complement(3119..>3643)
                     /locus_tag="VMZ53_05050"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HUS27848.1"
                     /translation="AALSMRRDMTELPTAWIVAAAVAWFCGFVVPSYLALVPKSGSMM
                     PRWQLGAAAAIVASIAFIVLGLVVHPSGQSSLDYSDRFAHGHWCLEIGLATAFVPVIV
                     GALFLRGALPVGSRWIAAALGAGGGCLGGLVLHLHCRVTDSLHVGVIHGGVVLVAAVL
                     SALLVPRATDRPLR"
BASE COUNT          606 a         1225 c         1176 g          636 t
ORIGIN      
        1 tggtcgacgt acggcgcagc gttttccgcc gcggattgga tcgtcgtcag caggccggcg
       61 cgatcgacgt tggcgtgcga caggaggcgc gcgagatatg ggaccacgcg cgcggtcgcc
      121 ggatagaccg atccttgatg ctcgatttcg gtcccgatcg agccgaggca atacctgcga
      181 acgagcgggt ctggagacgc cagcccgcgc aagtacgccg ggaattcgtc gacgcagtcg
      241 tcgtagcagc cgtgtgtgtc ggcccacggc tcggcgtcga cgccgatgag ctgatcggga
      301 agcggcagcg ccggctcgtc gatcttgaag agggcttctc cgagcttcca ctcgagccct
      361 tcgccggtga cgccgaagcg cagctcgagc ggcgtcacgt cgatctcgcg gcggagcgcg
      421 ccgctcgcgt cgaagatctt ccatgtgcca gtgagcttct cgccgttgtg accgccggtc
      481 gccgcgatcg ccttgccgtc gccgtgatag agcacgcatg cgagagcggg gccttctcgg
      541 cgcgcttcga gctcgagcac gccaccacga gcgcgctccg tcacgctcgc gatcgcatcg
      601 ccgtcgtacg tgcgaacgcg ctcctcgtgc agcgtgccgt cgcgcagcca gctgcctttg
      661 tacgtttgac ggcctttcgc gtcctcgcgg tatcgcgtcg agagcgagcc gtcgtcgaag
      721 tagtagtcgc gctcttcacc gtcggcggtg cgcgtcgtct tcttctgcag cgagccgtcc
      781 gcttcgtact ccgcgatcat cgtcgcttcg ccgctcgcgt cgcgcacttc ttcgtggcgg
      841 aggatgccgt cgcgtgacca ccatcgccag cgaccgactt gcttgttcgt gccgcgctcg
      901 atcgcgccgt cgacccagcg gtccatatcg ggaaaccagc gcgcgtcgct gctgacgctc
      961 ttggggcgcg cgggcagtgc gttcccgtcg gggccgcact cggtgccgtc gcgcaggaag
     1021 tagcggatcg tgtagttcgt cttgccgtcg cgcgtgtagt acttcgcgct ccacacgttt
     1081 ggcgctgctt gcgcgaacgg ctcgggtgat ggcgcctcgc ttcgataaaa cgcgctgttc
     1141 atgatcacgc cgtgctgcca ctcggcttcg gacgcgaccg tgccatctgg atggaagttg
     1201 atgttcttgc cgtggacctt gccgtggtcg taggtgcact cgccgtgcaa cacgccatcg
     1261 cgagtccagc tgcgatagag gccgtggcgt cgaccatcgc tgtcgagcac gccggagacc
     1321 cactcgaatc cgggatcttt gggatcccaa cgcgcgtcct ccgcgaccgt ggtggggcgc
     1381 acgggaggtg agttctccgg gttccccacg acgatgatgt tcgtcgcgca gcggcgcgag
     1441 atcaacccgt gaagggttag gagtggggcg gctacgtcgg cggcagttgc agcgactcga
     1501 gaaacggcac gacagccgct gcgacgcgtg cgcatccgcc gtgagagcgc gtgacagcgt
     1561 gcgatgtctc gagctcgatg cccgcgtact tctttccggc gagctcttgt cgcagcgacg
     1621 tacacacggc cggaccgacg ccgctgtaag gttggttcgc tcgcacactc aatccggcgc
     1681 cacgcagcat gaacatcagc cgttcggcga gctccgcttc gaattcgtgc gcagggtcgt
     1741 acagcacgcc gcagtcgtag gtgcgattgc tgggatcgag ctcgggcgcg aagctgtgcg
     1801 aagagaaatg cagcacagcg ccgcagtcga tgagacgagc actgacatcg cgccggatcg
     1861 catcccaata cggcgcatga aacagcgcaa tgcgcgcagc cttgtcaccc gccgataggt
     1921 gcacgttgcc aggcaccggc tcgccgtagc agatggcagg aatgacatcg gcgtggtctg
     1981 ggccgcggtt gaggtcgaca aacatccgcg agaacgcacc tgcgtggacg ggcaatccca
     2041 ccgcctccga cagccgcgac gcgatgtcga acgctccgtg atcccaaccc gcttgcgact
     2101 gcagcgcttc gacgggaacg ccgagatcga cgcccggcgg cagtgtccac gaggcatgct
     2161 cgcaggacag aacgaggccg atcacgttct caaggctagg tgtaggcatc gcggttgtcg
     2221 agctatgact cggttagcga cggatggccg aatctatttc ttcaaacctt cgaggaaggc
     2281 caggacgtgc gtccatgcgt cggccgagga ggtctcgtcg tacttcgggt tgctcgggtt
     2341 cgcgaatccg tgctcggcgt catagcggta gatctgcgcc gtcacgcctg ccgttttcag
     2401 tgcagcctcg agctcgtcga ccttcgcggg cggaatgccc ttgtccttgt tgccgaacac
     2461 gccgagcagc tggcccttga tcgcgccgag cttcgcggga tcggtctcga gctgaccgta
     2521 atagacaatc gcgccgtcga gctcgggatg cgcgagcgcg gtctgcagtg accaacctcc
     2581 gccgaagcac caaccgatca ccgccttctt cttcgcggcg atgcgcgagt ccttcgctag
     2641 aaattcgaac gccgcattga tcgtgccttt cgctttctct tgatcgacgg ccttcatcgc
     2701 agccatcgct tcatcaggcg tcgtcgcgac ggtgccgccg tacagatcga cggcgagcgc
     2761 agcccacccc gtcgccgcga gtcggtccgc ccagtgcttg atgttgccgt tgaggcccca
     2821 ccactcgtgg atcacgacga ttgcgggaaa cggtcccttg ccctgcggca agctcaagta
     2881 cgccttcgtc cccgcgaggt cgatcacctc gcccttcaga tgttgcggcg gcgtcgccgg
     2941 tggctcgtgc agcgccttga actccgcctc cgacagcgga cccgtcatcg ccggcgtgtt
     3001 ctgccccgtt gcctgcgtcg tcgtcgtcgt cgatggcggt ggcgtggacg agcccgaaga
     3061 acacgcagcg atcgcgagga gcaatacggc agttcgcatg ccgggcagtt accacggatc
     3121 agcgcagcgg acgatcggtc gcgcgcggaa cgagcaatgc gctgagcacc gcggccacga
     3181 gcacgacgcc accatgaatg acgccaacgt ggaggctgtc cgtcacgcga caatgcagat
     3241 gcagcacgag cccgccgaga catccgccgc ccgcaccgag cgcggctgcg atccaccgcg
     3301 acccgaccgg caacgcacct cgcaagaaca gcgcgccgac gatcaccggc acgaacgcag
     3361 tcgcgagccc gatctccagg caccagtgcc catgcgcaaa gcgatcgctg tagtcgaggc
     3421 tcgactgccc ggacggatgc acgaccaacc cgagcacgat gaatgcgatc gacgcgacga
     3481 tcgcggccgc tgcgccgagc tgccaacgcg gcatcatcga gccgctcttc ggcacgagcg
     3541 ccaaatagct cggcacgacg aagccgcaga accacgcgac cgccgccgca acgatccacg
     3601 ccgtcggcag ctccgtcatg tcgcgccgca tgctgagcgc cgc
//
LOCUS       DATLBZ010000096        13369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3021982, whole genome shotgun sequence.
ACCESSION   DATLBZ010000096 DATLBZ010000000
VERSION     DATLBZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3021982"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(481..1212)
                     /locus_tag="VMZ53_05055"
     CDS             complement(481..1212)
                     /locus_tag="VMZ53_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HUS27849.1"
                     /translation="MAIGLAAAIVFIIDAWMPFGSTILYPLTLFTTWVHEMGHGLAAL
                     LQGGEFTEIVINKNAGGYAMAFASRGWGQAIVCAGGMLAPPLLGSAIIATVHGPRRAR
                     IVLAALAIAIVVSLLIYVRSATGVIALPFVAALLGWSATLGFRKNPHRRVVMAQVLGV
                     LLAVDTVTRMVGYALSTEARKGEASDVQLMANEVGGPYWLYGLAIIAIALSALALSLW
                     WAWRRPAAPAAVDRSPARRASKAAR"
     gene            complement(1253..1519)
                     /locus_tag="VMZ53_05060"
     CDS             complement(1253..1519)
                     /locus_tag="VMZ53_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27850.1"
                     /translation="MAAAMDPAYEELFLGIAHALFVNRLHVLRLTEVVRLGIKPDPHD
                     QNMEVPDTIDRELIQQAFAYVQRHFPPSFSGKIESAKARWIRLA"
     gene            complement(1557..3119)
                     /locus_tag="VMZ53_05065"
     CDS             complement(1557..3119)
                     /locus_tag="VMZ53_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS27851.1"
                     /translation="MSPLRNAITLLVIAATVVGCSANRRRTPDDTLVLLFDGPITTAD
                     PRFAKNNYDSKLSRLVAPGLITVDTPDAAPQLELADKIERIDDLTVDVLVRADAKFSD
                     GKPVRAEDVARTFQSVMDDKCKSLYQGNLLERFTSVEAVDDKRVRFRFKKKLATFMTD
                     IEFGVVSFHDTPPGECRGRKLIGAGPFALRSVSANSAFLDRNAYYRTPPSIAHVDIRV
                     VRDPAARILMLVGGSADLVQNGVRPDLVDEVIKRPRVQKQSGASVILTYMLINNTDEV
                     LKDHRVREALALAIDREEIIRAEFGGRAVLATGLLPPNHWAYNPDVTRYPHDIARANQ
                     LLDEAGYKRDARGIRRDAKGKPFELVYKTSADPFRVTVARIIGAQLREVGIVVEIRSF
                     EFSTFFADVKAGQYQIATMQSTEITEPDFYMTFFHSTRWPTKKDPDGYNRWRYKNPAI
                     DKLVEEGRAELDPARRKAIYNEVQKIIAADLPIIPLWHEDNVVLSNTTVQGYEIVPDA
                     RFRGLVSASKSL"
     gene            3110..4729
                     /gene="dacB"
                     /locus_tag="VMZ53_05070"
     CDS             3110..4729
                     /gene="dacB"
                     /locus_tag="VMZ53_05070"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HUS27852.1"
                     /translation="MVTSRVGRGLMILLALVSIDTGRAQSQGSGASSGSAGGSATATP
                     TTDVDDEEGGAGSGSALVAPKDPAQRGSWLVGQLDAAINARPLLSSRAKIGVSMVDLS
                     TGAELYAHDADTPMSLASNAKLLTSVAALGTLGGGFRWRTAVLIDDKALDETTGIVAG
                     DLYIKGRGDPTLGSADLRALAIEVAARGVREIQGKLVIDDTYFDADVEAPHFAEQPKE
                     RAAFRAPVASFGVSRSAFLVNVIGEPGGGAKVWLEPDAGDSIKLTKTEVTSISVGRTR
                     IKVDIKPRKGSKTGELDVEVTGQIRFADGHWYAKRRIDNPTQYAGQVFKAALAEQGIK
                     LTKKAITLGAIPVNAKLLTAHDSAPLSQVIREMNKSSDNYFAETVLKTIGAETRATPG
                     PATWADGHAAVQTFLATLGLPPGTYRADNGSGLFNATEVSPKQMVTLLKGAHADYRIG
                     GDLVASLPVGGQDGTLAKRWRNRAGAGRVRAKTGTLEKVATLAGYVAVDSNHVVAFSI
                     LVNDIPTGQKGPARAMADDMVDALVAYLDAK"
     gene            4765..5187
                     /locus_tag="VMZ53_05075"
     CDS             4765..5187
                     /locus_tag="VMZ53_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HUS27853.1"
                     /translation="MAELKGSKTHSNLKDAFAGESQANRRYLYFAKVADVEGHPDVAG
                     LFRDTAEGETGHAHGHLDYLKRVGDPATGEPIGDTALNLKASIAGETYEYTQMYPGFA
                     KTAREEGFTEIAEWFETLARAERSHAGRFQKGLDTLNK"
     gene            5233..5733
                     /locus_tag="VMZ53_05080"
     CDS             5233..5733
                     /locus_tag="VMZ53_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUS27854.1"
                     /translation="MRKLDPLALDDFETQPFRLVVTAQLDADPLSVFAELRDPSLFFP
                     LLYKSVWKTGATSGVGAEREVRVRTFGTFRERMLAWDEGERVAFAMVETTSPLIDRMA
                     EDWQLSRDGIYTRLTWSVFATPSRIGRFATPALKVILRTLFMRAATNLQKRAGTLKTE
                     RAKNVS"
     gene            5730..6323
                     /locus_tag="VMZ53_05085"
     CDS             5730..6323
                     /locus_tag="VMZ53_05085"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HUS27855.1"
                     /translation="MTDLVRPITRRDIKGPAMYAHIRDDYRKRVIEMKQARRVFIGDI
                     VELVFDNRLTLTMQIEEICRLENLTRDEQIEEEIRITNELMPTPSSLAATIFVALPHD
                     DPKTIERMRELVGLDENVILHIGPHAIRADFEKGRSTDEKISAVQYTRYPLSADAKQA
                     LLTPGTRIEVQIDHPAYRHRVQCSEDLRASLAADYGD"
     gene            6324..6860
                     /locus_tag="VMZ53_05090"
     CDS             6324..6860
                     /locus_tag="VMZ53_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27856.1"
                     /translation="MRQGWVLFAVVTTGGAACGGAQSRANREQENFNCRDRYASYVAT
                     GHISGDELGVQMDCAEAGPRVKRWRTDRQGKREEDGHAITPGDFERAWKEINGTGWEN
                     LRDCGNGSLEKRDPVYQFDIKDDQNTASFSCQTREVPYPYNDITDALDLMAQKGSGQL
                     GDDEPDDAKALDKKDKQK"
     gene            6857..7558
                     /locus_tag="VMZ53_05095"
     CDS             6857..7558
                     /locus_tag="VMZ53_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS27857.1"
                     /translation="MTGPLSGSTAVVTGASRGIGRAIAMRLAEAGAEVALWARDSTAL
                     RMVASEIAANRGKARSIVVDVSDADAVNRAADLVRATMPMLRTVVNNAGVVLRKSTEA
                     VTDDEWRKVMAVNLDGTFYVTRAFIPDLERNQGRVINISSISGQEGTSMLAAYCAAKH
                     GVIGLTRALAEELRTAKVSVNAICPGSVDTAMLREGMPGARPDMTPDDIAKTALFLAH
                     AAPQALTGACIDVYG"
     gene            7576..8256
                     /locus_tag="VMZ53_05100"
     CDS             7576..8256
                     /locus_tag="VMZ53_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HUS27858.1"
                     /translation="MPTSSHDTLSPPDSKALEALPIFPLPNCVLLPGGLLPLHVFEPR
                     YRELTRDCLGSHQLMAVARLRPGYETTYYGRPPVYERCGVGRIICSEELPDGRFALLL
                     RGVARVEIARELPQERAYRRVEARVLSDAEHNRAEALETHRKLVKLCDRLAEVIEKGG
                     PQLRDLVRSFDDPGVCADAIAAALVMDADARQELIETCNPMVRLQRTLGHVSHMLCEL
                     APCDGTVN"
     gene            complement(8257..9345)
                     /locus_tag="VMZ53_05105"
     CDS             complement(8257..9345)
                     /locus_tag="VMZ53_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27859.1"
                     /translation="MGRILAAIVILAACGGGAADDDTSPPGPDPQLGPPAGNPDGHCT
                     VPGEAMPASVASPRTVVGTGTPESCTSAAFVAAVAAGGVITFDCGAEPVTIELDQTAK
                     VVNDTGPEIVIDGGTKVTLSGGGRHRILYMNTCDQAQKWTTSHCQNQDEPHLTVQNLT
                     FVDGNSKGEDTDGGGGGAIFVRGGRFKIVHSRFFNNVCDDTGPDVGGAAVRVLSQYET
                     RPVYVVDSTFGGADGYGNTCSNGGGLSSIGVSYSVINTLFSHNKAIGNGANPAKSGTP
                     GGGSGGGIYNDGNTFTLSLCGVAMHDNTANEGGGAIFFVSNDRSGNLVISDSVLSHNA
                     SAGFETQGFPGIFVLASGPPTVTNSTLE"
     gene            9376..9834
                     /locus_tag="VMZ53_05110"
     CDS             9376..9834
                     /locus_tag="VMZ53_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27860.1"
                     /translation="MWRVLAVGLAVIGCGDPAPATTDAAADAETDAFVIRRTSPFVMQ
                     LCTSGNPGDVTCPLNSVALDELGGPAVGARLTFTAKPMGQDMVLDDIEIAGVAHLHIA
                     GLQFEQWTSGTPTRVGVYIESLDAGSSVGPVTIEMFGASSQVGIRVEAID"
     gene            complement(9862..11235)
                     /locus_tag="VMZ53_05115"
     CDS             complement(9862..11235)
                     /locus_tag="VMZ53_05115"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HUS27861.1"
                     /translation="MAKTLADGEKVEVQGSSSTYELHRQGTVYSCSCPAWRNQGAQIG
                     FRTCKHLRAYLGDENETRRIGSPAPQSSSRSSSRSSGSSSAAAGAASGSSTGDGKAPP
                     VLLAHKWETDHDPTGWWMSEKLDGIRAYWDGEGFISRLGNKFYAPDWFIADLPADTLD
                     GELWVGRKMFQKTTSIVRSGAQSDDWKQVTYVVFDAPNAKGGFEQRLAHAQKVLKKAA
                     APHARFLDHITCEGVDHLREELQRVEALGGEGLMLRQPKSPYVAGRSTTLLKVKTFHD
                     AEARVIGHAPGTGKHKGRLGALIVELSDGTKFNVGTGFSDAEREDPPAVGAVITFRYQ
                     ELSNDGVPRFPSYVGERLDVKTPAKIGASTAKPPAAAKIAAVPPKTSKAAPAQTKNVA
                     GFSVSLVHQAEQKWWKIEVKGTAHYTSFGKIGSPGQTRVSDLGSATAAESDADKRAQQ
                     KRKEGYA"
     gene            complement(11305..11922)
                     /locus_tag="VMZ53_05120"
     CDS             complement(11305..11922)
                     /locus_tag="VMZ53_05120"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HUS27862.1"
                     /translation="MSNWTAHLEFEEGTSSKFWRARVEGATLYVNYGKIGTAGQTQVK
                     DFGSADLATKEYDKLVREKRKKGYVDAGSGGAAAATVDDDEVNDDRQDDDEDAGDDAG
                     GDDDDDAPKPKRPQVPNPASAARGPAAPAGTAARPAAAAPAARPPGSIRLLLETGNRK
                     VETWISLVGGEVRMESTEKYATPDAAKKAHERLKKMLLAEGYKES"
     gene            complement(11919..13223)
                     /locus_tag="VMZ53_05125"
     CDS             complement(11919..13223)
                     /locus_tag="VMZ53_05125"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HUS27863.1"
                     /translation="MAEQAQPQWADVAAAGGIREWVDAQVAKQGLSDPGASQSMSDAE
                     KKLYKARREEERRVRRELFKQAWAAYKRSHIVHLGPGVWWHDTADVDRFDPEDPDKKR
                     QDNALPAINDVDAFAKALGMTVAKLRWLAFHRDVDSGTHYHRWLVPKRDGTPRLISAP
                     KPDLKAAQRWITRQVTEHLPVHGAAHGFLTGRSILTNAVVHAGAKIVVKMDIRGFYPT
                     VTWRRVKGLLRRAGLGEQVATLMALLATESPREEVATHGKTYFVATGPRSLPQGAPTS
                     PSITNALCLRLDARLSGLAKKLECRYTRYADDMTFSWHGDAKPDIGNLIHAVSAIVKA
                     EGFAIHPKKTRVMRSGARQSVTGLIVNHAPPDRPIARVPRKRIRDLESAIYNREKGRP
                     GKGETLEQLKGLAAFVMMTDRAKGQALMERLDKLIAARNPTP"
BASE COUNT         2259 a         4315 c         4433 g         2362 t
ORIGIN      
        1 ctgcatgact ggtctcctcg gtttgcgcct cgagcgcgtt ataggttgag gcacggtaac
       61 tcgtgacctc gtagccgacg agaccagtca ctctcgctcg cgcaagcgtc gccacaacat
      121 cctctctctc cccgccggcc cggcgctctc ggccaagtaa gagaccgcgc ctcgctgcga
      181 gccgtagctc gcgcgacgag ccgcgtagat cgcatcgcac gcggcagcgc tccacgacac
      241 cgcgacgtgt tgcgtgcatc gaacgcgcac ttcacaagat cagcgacgcg gtctcttcgt
      301 gcccacgtgg gcacgaacgt cggtcacagc ggcacgcgcg tgcccacatg ggcacgaacg
      361 tcggccacag ccgcaagcgc ctctatcgat cgttggcagc tgcgtaggcg agcgtcacaa
      421 caccgcgctc cgcgacgcgg accgcagcgc aggagccgct cgacgcccgc gcggacggct
      481 ttatcgagca gctttactcg cgcggcgcgc cggcgatcga tcgacggctg cgggcgccgc
      541 cggccgccgc cacgcccacc acaggcttag cgcgagcgcg gacagcgcga tcgcgatgat
      601 cgcgaggccg tagagccagt acggaccgcc gacctcgttc gccatcagct gcacgtcgga
      661 ggcttcgcct ttgcgcgctt cggtcgacag tgcgtagccg accatgcgcg tcaccgtatc
      721 cacggcgagc agcacgccga gcacttgcgc catcacgacg cggcgatgcg gattcttgcg
      781 gaagccgagc gtcgccgacc acccgagcag cgcggcgacg aacggcagtg cgatcacgcc
      841 cgtggcggag cgcacgtaga tgagcaggct gacgacgatc gcgatcgcga gtgccgcgag
      901 cacgatgcgc gcgcgacgcg ggccgtggac cgtcgcgatg atggcggagc cgagcagcgg
      961 cggtgcgagc attccgcccg cgcagacgat cgcttggccc cagccgcgcg acgcgaacgc
     1021 catcgcgtag ccgcctgcat tcttgttgat cacgatctcc gtgaactcgc cgccttgcag
     1081 cagcgccgcg aggccgtggc ccatctcgtg gacccacgtc gtgaagagcg tcagcggata
     1141 gaggatcgtg gagccgaacg gcatccacgc atcgatgatg aacacgatcg ccgccgcgag
     1201 gccgattgcc atccgcggtt gcatcgacct ggccacagcc agctatgcca cgctaggcga
     1261 ggcggatcca gcgcgccttc gccgactcga tcttgccgga gaaggagggc gggaagtgac
     1321 gctggacgta cgcgaacgct tgctggatca gctcgcgatc gatcgtgtcc gggacttcca
     1381 tgttctggtc gtgcgggtcc ggcttgatgc cgagtcggac gacctcggtc agccggagaa
     1441 cgtgcagtcg attaacgaac agcgcgtggg cgataccgag aaacagctct tcgtacgcag
     1501 ggtccatcgc tgcagccatc gagggtgtgt aactaccacg gtcgggctcg cgggagctag
     1561 agggacttcg aggcagaaac caggccgcgg aagcgcgcat ctggaacgat ttcataccct
     1621 tgcacggtcg tgttcgagag gacgacgttg tcctcgtgcc acagcggaat gatcggcagg
     1681 tcggccgcga tgatcttctg gacctcgttg tagatggcct tgcggcgggc tggatcgagc
     1741 tcggccctgc cctcctcgac gagcttgtcg atcgccgggt tcttgtagcg ccaacggttg
     1801 tagccgtcgg ggtccttctt cgtcggccag cgcgtcgagt ggaagaacgt catgtagaag
     1861 tcgggctcgg tgatctcggt gctctgcatc gtcgcgatct ggtactggcc cgctttgacg
     1921 tcggcgaaga atgtcgagaa ctcgaacgag cgaatctcga ccacgatgcc gacctcgcgc
     1981 agctgcgctc caatgatgcg cgcgaccgtc acgcgaaatg gatcggcgct cgtcttgtag
     2041 accagctcga atggcttgcc cttcgcgtcg cgccggatgc cgcgagcgtc acgcttgtag
     2101 ccggcttcgt cgagcagctg attcgcgcgc gcgatgtcgt gcgggtagcg cgtgacgtcg
     2161 gggttgtacg cccagtgatt cggcggcaag aggcccgtcg cgagcacggc ccgcccgccg
     2221 aactccgcgc gaatgatctc ctcgcgatcg atcgcgagtg ccaacgcctc acgcacgcga
     2281 tgatctttga gcacctcgtc ggtgttgttg atcagcatgt acgtgagaat cacgctcgcg
     2341 ccggactgtt tctggacgcg cgggcgcttg atcacctcgt cgacgagatc gggacgcacg
     2401 ccattctgca cgaggtccgc cgagccgccg acgagcatca ggatgcgcgc cgctggatca
     2461 cgaacgacgc ggatatcgac gtgcgcgatg ctcggtggcg tccggtagta cgcgttgcga
     2521 tcgaggaacg cgctgttggc cgacacgctg cgtagcgcga acggtcccgc gccgatgagc
     2581 tttctgccgc ggcactcacc cggcggcgta tcgtgaaacg acaccacgcc gaactcgatg
     2641 tccgtcatga acgtcgcgag cttcttcttg aagcggaagc gcacgcgctt gtcatcgacc
     2701 gcttcgacgc tcgtgaaccg ctcgagcagg ttgccttgat agaggctctt gcacttgtcg
     2761 tccatcaccg actggaacgt gcgcgcgaca tcctcggcgc gaaccggttt gccgtcggaa
     2821 aatttggcgt cggcgcgaac gagcacgtcg accgtcaggt cgtcgatgcg ctcgatcttg
     2881 tcggcgagct cgagctgcgg tgctgcgtcg ggtgtgtcga ccgtaatgag cccgggtgca
     2941 acgaggcgcg agagcttgct gtcgtagttg ttcttcgcga agcgcggatc ggccgtcgtg
     3001 atcggcccgt cgaacagcaa gacaagcgtg tcatcgggcg tacggcgacg gttcgcgctg
     3061 cagccgacta cggtcgcggc aataaccagc agcgtgatag cgtttctgag tggtgacatc
     3121 gcgcgtcggt cgcgggctga tgatcctgct cgcgctcgtc agtatcgaca cgggccgggc
     3181 acagtcgcaa ggaagcggcg caagctcggg atccgccggc ggttccgcaa cagcgacacc
     3241 gacgaccgac gtcgacgatg aagaaggcgg tgccggctct ggcagcgcgc tcgtcgctcc
     3301 gaaagatcct gcgcagcgcg gctcgtggct cgtcggccag ctcgacgccg cgatcaatgc
     3361 acggccactc ttgagctcgc gcgcgaagat cggcgtctcg atggtcgatc tctcgacggg
     3421 cgcggagctc tacgcccacg acgccgacac accgatgagc ctcgcctcga acgcgaagct
     3481 gctcacgtcg gtcgctgcgc tcggcacact cggcggcggc tttcgctggc gcaccgcggt
     3541 gctcatcgac gacaaggcgc tcgacgagac gaccggtatc gtcgcgggcg acctctacat
     3601 caagggccgc ggcgatccga cgctcggctc ggcggatctg cgtgcgctcg cgatcgaagt
     3661 ggctgcgcgt ggcgtgcgcg agatccaagg caagctcgtc atcgacgaca cctacttcga
     3721 cgccgacgtc gaggcaccgc acttcgccga gcagcccaag gagcgcgccg catttcgtgc
     3781 accggtcgcg agctttggcg tgtcgcgcag cgcgtttctc gtcaacgtga tcggcgagcc
     3841 aggcggcggc gcgaaagtat ggctcgagcc cgacgcgggc gactcgatca agctcacgaa
     3901 gacggaggtc acgagcatct ccgtcggtcg cacgcgcatc aaggtggaca tcaagccgcg
     3961 caaaggcagc aagaccggcg agctcgacgt cgaggtcacc ggccagatcc gcttcgccga
     4021 cggccattgg tacgccaagc gccgcatcga caacccgacg cagtacgccg gccaagtctt
     4081 caaagcggcg ctcgccgagc aaggcatcaa gctgacgaag aaggcgatca cgctcggcgc
     4141 gattccggtc aacgcgaagc tgctcaccgc gcacgacagc gcgccgctct cgcaagtgat
     4201 ccgcgagatg aacaagtcga gcgacaacta cttcgccgag accgtgctga agacgatcgg
     4261 cgcagagacg cgcgcgacgc caggcccggc gacatgggcg gatggtcacg cggcggtgca
     4321 gacgtttctg gcgacgctcg gcctgccgcc tggcacgtat cgcgccgaca acggctcggg
     4381 cctgttcaac gcgaccgagg tctcgccgaa gcagatggtc acgctgctga agggcgcgca
     4441 cgcggactac cggatcggtg gtgacctcgt cgcgtcgctg ccggtcggcg ggcaagacgg
     4501 cacgctcgcg aagcgctggc gcaatcgtgc gggagctggc cgcgttcgcg cgaagacggg
     4561 cacgctagag aaagtcgcga cgctcgccgg ctacgtggcg gtcgacagca atcacgtcgt
     4621 cgcattttcg atcctcgtca acgacatccc gacgggacag aaaggtcctg cgcgcgcgat
     4681 ggcggacgac atggtcgatg ccctcgtggc ctacctcgac gcgaagtagt gtggtacgac
     4741 ggctccacca aaaggagcct tacgatggcc gagctcaagg gcagcaagac ccattcgaat
     4801 ctcaaggatg cgttcgcggg tgagtcgcaa gcgaaccgcc gttatctcta tttcgcaaag
     4861 gtcgccgacg tcgagggtca ccccgatgtg gcgggcctgt tccgcgacac cgccgagggc
     4921 gagaccggcc acgcgcatgg tcacctcgac tatctgaagc gcgttggtga tccggcgacc
     4981 ggcgagccga tcggcgacac cgcgctcaac ctcaaggcgt cgatcgcggg cgagacgtac
     5041 gagtacacgc agatgtaccc gggcttcgcg aagaccgcac gcgaagaggg cttcaccgag
     5101 atcgccgagt ggttcgagac cctggcgcgc gccgagcgtt cacacgctgg ccgcttccag
     5161 aagggcctcg acaccctcaa caagtagtcg tcgtcgctgg ccctcgcggg ccacacagct
     5221 aggatgcggg tcatgcgaaa gctcgacccg ctcgctctcg acgatttcga gactcaaccg
     5281 tttcgactcg tcgtgacggc gcagctcgat gcagacccgc tctcggtgtt tgcagagctg
     5341 cgcgatccgt cgctgttctt cccgctgctc tacaaatcgg tgtggaagac cggcgcgacg
     5401 agtggcgtgg gcgccgagcg cgaagttcgc gtgcgcacgt tcgggacctt ccgcgagcgc
     5461 atgctggcgt gggacgaagg cgagcgcgtc gcatttgcga tggtcgagac gacgtcgccg
     5521 ctgatcgatc gcatggcgga agactggcag ctctcgcgcg atggcatcta cacgcggctg
     5581 acgtggagcg tgttcgcgac gccgtcgagg atcggccgct ttgcgacgcc cgcgctcaag
     5641 gtcatcctgc gcacgctgtt catgcgcgct gcgacaaacc tccagaagcg cgccggaaca
     5701 ctgaagaccg agcgtgctaa gaacgtttca tgactgacct cgtgcgaccc attactcgtc
     5761 gggacatcaa aggtcccgcg atgtacgcgc acattcgcga cgactaccgc aagcgcgtga
     5821 tcgagatgaa gcaggcgcgg cgtgtgttca tcggcgacat cgtcgagctc gtgttcgaca
     5881 accggctcac gctcacgatg cagatcgaag aaatctgccg gctcgagaat ctgacgcgcg
     5941 acgagcagat cgaagaagag attcgcatca cgaacgagct gatgccgacc ccgagctcgc
     6001 tcgcggcaac aatctttgtc gcgcttccgc atgacgatcc gaaaacgatc gagcgcatgc
     6061 gcgagctcgt tggcctcgac gagaacgtca tcctccacat cggcccgcac gcgattcgcg
     6121 ccgacttcga gaagggccgc tcgacggacg agaaaatctc ggccgtgcag tatacgcgct
     6181 atcccctctc agccgacgcg aaacaagcgc tgctgacccc gggcactcgg atcgaggtgc
     6241 agatcgacca ccctgcgtat cgccaccgcg tacaatgttc cgaggatctg cgggcgtcgc
     6301 tcgcagccga ctacggagac tgaatgcgtc agggctgggt gcttttcgcg gtcgtcacca
     6361 ccggtggcgc ggcatgtggt ggtgcgcagt cgcgcgccaa ccgagagcag gagaacttca
     6421 actgccgcga tcggtatgcg agttacgtcg cgaccggtca catcagcggc gacgagctcg
     6481 gtgtgcagat ggactgcgcc gaagctggac cgcgcgtcaa acgctggcgc acggatcgtc
     6541 agggcaagcg cgaagaggac ggccacgcga tcaccccggg cgatttcgag cgcgcctgga
     6601 aagagatcaa cgggacgggc tgggagaacc tgcgcgactg cggcaacggc agcctcgaga
     6661 agcgcgaccc ggtctatcag ttcgacatca aggacgatca aaacacggcg tcattctcgt
     6721 gccagacgcg cgaggtgccc tacccgtaca acgacatcac cgacgcgctc gacctgatgg
     6781 cgcaaaaagg cagcggccag ctcggcgacg acgaaccgga cgacgcgaag gcgctcgaca
     6841 agaaggacaa gcagaaatga ccggaccgct ctccggctcg acagccgtcg tcaccggtgc
     6901 gagtcgcggc atcggtcgag cgatcgcgat gcggctcgcg gaagcaggtg cggaggtcgc
     6961 gctttgggcc cgcgactcga cagcgctgcg catggtcgcc tcggagatcg cggcgaatcg
     7021 cggcaaagcg cgctcgatcg tggtcgacgt cagtgatgcg gacgccgtca accgcgccgc
     7081 agatctcgtt cgcgcaacga tgccgatgct gcgtacggtc gtgaacaacg caggcgtcgt
     7141 gctgcgaaaa tccaccgaag cagtcaccga tgacgagtgg cgcaaggtga tggcagtcaa
     7201 cctggacgga actttctacg taacccgtgc attcatcccg gacctcgagc gcaatcaggg
     7261 acgcgtcatc aacatctcgt cgatctcggg acaagaggga acctcgatgt tggccgccta
     7321 ctgcgcggca aagcacgggg ttatcggact cacgcgtgcc cttgccgagg agttgcgtac
     7381 tgctaaggtc tcggtgaatg cgatctgtcc gggctctgtc gacacggcga tgttgcgtga
     7441 agggatgccc ggagcgaggc ccgacatgac gccagacgac atcgcgaaga ctgcgttgtt
     7501 tcttgctcac gccgcgccgc aagcactgac tggtgcttgt atcgacgtgt acgggtaaaa
     7561 cgagactcgg agaccatgcc gaccagctcg catgacacgc tgtctcctcc cgactcgaag
     7621 gcgctcgaag cgttaccgat ttttccactt ccgaactgcg tgctgttgcc gggtggtttg
     7681 ctgccgctgc acgtgttcga gccgcgctac cgcgagctga cgcgcgactg tctcggcagt
     7741 caccagctga tggcggttgc ccgccttcgc cccggttacg agacgacgta ctacggtcgc
     7801 ccgccggtct acgagcgctg cggcgttggc cgcatcatct gctctgaaga gctgcccgat
     7861 ggccgcttcg ccctgctcct tcgcggtgtc gcccgcgtcg aaattgcgcg cgagcttccg
     7921 caggaacgtg cgtatcgccg agtcgaagca cgcgtgctca gcgatgccga gcacaaccgc
     7981 gccgaagcgc tcgagacaca tcgcaagctc gtgaagctct gcgaccgcct cgccgaggtg
     8041 atcgaaaagg gcggcccgca gctgcgcgac ctcgttcgct cgtttgacga ccccggtgtt
     8101 tgcgccgacg cgatcgccgc tgcgctcgtg atggatgccg atgcccgcca agagctgatc
     8161 gaaacgtgca atccgatggt gcgcctgcag cgtacgctcg gccatgtcag ccacatgctg
     8221 tgtgagctgg ctccgtgcga cggcaccgtc aactgatcac tcgagcgtcg agttggtcac
     8281 ggtcggcggc cccgacgcga gcacgaagat gccgggaaag ccctgcgtct cgaagcccgc
     8341 gctcgcattg tgcgacagca ccgaatcaga gatgacgagg ttgccgctgc gatcgttgct
     8401 cacgaagaag atcgcgccgc ctccctcgtt cgccgtgttg tcgtgcatcg cgacgccgca
     8461 cagcgagagc gtgaacgtgt tgccgtcgtt gtagatgccg ccgccgctgc caccgccggg
     8521 tgtccccgac ttcgctggat tcgcgccgtt gccgatcgcc ttgttgtggc tgaacagcgt
     8581 gttgatgacg gagtacgaga cgccgatgct cgagagcccg ccgccgttgc tgcacgtatt
     8641 gccgtaaccg tctgcgccgc cgaacgtcga gtcgacgacg tacacggggc gcgtctcgta
     8701 ctggctgagg acgcgcacgg cggcgcctcc cacgtcgggt cccgtatcgt cacaaacgtt
     8761 gttgaagaag cgcgagtgca cgatcttgaa gcgaccgccg cgcacgaaga tcgcgccgcc
     8821 gccaccgccg tcggtgtcct cgcccttcga gttgccgtcg acaaacgtca ggttctgcac
     8881 ggtcaggtgc ggctcgtctt ggttctggca gtgcgaggtc gtccacttct gcgcctgatc
     8941 gcacgtgttc atgtagagga tgcggtgccg cccgccgccc gacagcgtga ccttggtgcc
     9001 gccatcgatc acgatctctg ggccggtgtc gttcacgacc ttcgcggtct gatcgagctc
     9061 gatcgtgacc ggctccgcgc cgcaatcgaa ggtgatgacg ccgcccgccg cgaccgccgc
     9121 aacaaatgca gcgctggtgc acgactctgg cgtgccggtg ccgacgacgg tgcgtgggct
     9181 cgcaacgctc gcgggcatcg cctcgccggg gaccgtgcag tgcccgtccg ggtttcccgc
     9241 cggtggccca agctgtggat cgggcccggg tggtgatgtg tcgtcgtcgg cggcgccgcc
     9301 accgcacgca gcaaggatca cgattgccgc gagaatgcgc cccatgtgtc catcatagtc
     9361 ggtacgctcg atcgcatgtg gcgcgttctc gcggtgggcc tggcggtgat cggttgcggt
     9421 gatccggcgc ctgccacgac ggatgcggcg gcggatgccg agacggatgc gttcgtcatt
     9481 cgccgaacgt cgccgttcgt gatgcagctg tgcacgagcg gcaatccagg cgacgtgacc
     9541 tgcccgctca acagcgtcgc gctcgacgag ctcggcggcc cagcagttgg cgcgcggctg
     9601 acgttcactg cgaagccgat gggccaagac atggtgctcg atgacatcga aatcgcgggc
     9661 gtcgcgcacc tgcacatcgc cggcttgcag ttcgagcaat ggactagcgg gacgccaacg
     9721 cgcgtaggtg tctacatcga gtcgctcgac gcggggtcgt cggtaggtcc cgtcacgatc
     9781 gagatgttcg gcgcgtcatc tcaggttggg atccgcgtcg aggcgatcga ctgagtagag
     9841 gtcgtcagcg gtaggcgcgg gctaggcgta gccctctttg cgtttctgct gcgctcgctt
     9901 gtcggcgtcg gactcggcgg cggttgcact acctagatcg ctcacgcgcg tttggccggg
     9961 gctgccgatc ttgccgaagc tcgtgtagtg cgcggtgccc ttcacttcga tcttccacca
    10021 cttctgttcg gcctgatgga cgaggctgac gctgaagccc gcgacgttct tcgtttgagc
    10081 gggtgccgct ttgctcgtct tcggtggaac cgctgcgatc ttggcggccg ccggtggctt
    10141 cgcagttgat gcgccgatct tggctggcgt cttcacgtcg aggcgttcgc cgacgtagct
    10201 cgggaagcgc ggcacgccgt cgttcgacag ctcttggtag cggaacgtga tcacagcgcc
    10261 gacggcgggc gggtcttcgc gctccgcgtc ggagaagccg gtacccacgt tgaacttcgt
    10321 gccgtccgag agctctacga tgagcgcgcc gaggcggcct ttgtgcttgc ccgtgccggg
    10381 cgcgtggccg atgacgcgag cttctgcgtc gtggaaggtc ttcaccttga gcagcgtcgt
    10441 cgagcgaccc gcgacgtatg gcgatttcgg ctggcgcagc atgaggcctt cgccgccgag
    10501 cgcctcgacg cgctgcaact cttcgcgcag gtggtcgaca ccttcgcacg tgatgtgatc
    10561 gaggaagcgc gcgtgcggcg ctgccgcctt cttgagaacc ttttgcgcgt gcgcgagccg
    10621 ctgttcgaag ccgcctttcg cattcggcgc gtcgaacacg acgtacgtga cttgtttcca
    10681 atcgtcggac tgcgcgccgg agcgcacgat gctcgtcgtc ttctggaaca tcttgcggcc
    10741 gacccacagc tcgccgtcga gcgtgtcggc gggaagatcg gcgatgaacc agtcgggcgc
    10801 gtagaacttg ttgccgaggc gcgagatgaa accctcgcca tcccagtatg cgcggatgcc
    10861 gtcgagcttc tcgctcatcc accagcccgt cgggtcgtgg tcggtttccc acttgtgggc
    10921 gagcagaacg ggcggcgctt tcccgtcgcc tgtcgacgat ccggacgcag cgcccgctgc
    10981 cgccgacgac gagcccgatg accgcgacga cgagcgcgac gaagattgcg gcgctggcga
    11041 accgatccgg cgcgtctcgt tctcgtcacc taggtacgca cgcaggtgct tgcacgtgcg
    11101 aaagccgatc tgcgcgcctt ggttgcgcca cgcagggcag ctgcacgagt agaccgtgcc
    11161 ctggcgatgc agctcgtacg tgctgctcga gccctgaacc tcgaccttct ctccgtcggc
    11221 tagcgtcttg gccatcgcga cgattctacc tgcgtgcgaa atggggccgg cgccctttcc
    11281 tgcacgaagc gccggatctt cttttcagct ctctttgtag ccttcggcga ggagcatctt
    11341 cttcaatcgc tcgtgcgctt ttttcgcagc atcgggagtc gcgtacttct ccgtggattc
    11401 cattcgaacc tcgccaccga cgagcgagat ccaggtctcg actttgcgat tccccgtctc
    11461 gagcagtagg cggatggatc cgggtggacg ggcggcgggc gcagcggcgg ccgggcgagc
    11521 ggcggtgcca gcgggggcgg cggggcctcg ggcagcggag gccgggttcg gaacctgcgg
    11581 acgcttcggc tttggcgcgt cgtcgtcatc gtcaccgccc gcgtcatcgc ccgcgtcttc
    11641 gtcatcgtcc tggcggtcgt cgttgacctc gtcgtcatcg acggtcgccg ctgccgcgcc
    11701 gcccgaaccg gcatcgacgt agcccttctt gcgcttttcg cggacgagct tgtcgtactc
    11761 cttggtcgcg agatccgcgg agccgaagtc tttgacctgc gtctgaccgg ccgtgccgat
    11821 cttgccgtag ttgacgtaga gcgtcgcgcc ctcgacgcga gcgcgccaga acttgctcga
    11881 cgtgccttct tcgaactcga gatgtgcagt ccagttgctc atggagtcgg gttccttgca
    11941 gcgatgagct tgtcgaggcg ttccatcagt gcctggcctt tcgcgcggtc cgtcatcatg
    12001 acgaatgccg cgaggccttt gagctgctcg agagtttcgc cttttccggg gcgacccttc
    12061 tcgcggttgt agatcgcgga ctcgagatcg cgaatgcgct tgcgaggcac gcgcgcgatc
    12121 gggcgatcgg gcggcgcgtg gttgacgatg aggccggtga cggactggcg tgcaccggag
    12181 cgcatgacgc gcgtcttctt gggatggatg gcgaagccct cggctttgac gatcgcgctc
    12241 accgcgtgga tgagattgcc gatgtcgggc ttggcgtcgc cgtgccacga gaacgtcatg
    12301 tcgtcggcgt aacgcgtgta gcggcactcg agcttcttcg cgagacccga gagccgcgcg
    12361 tcgagacgga ggcacagcgc gttggtgatc gacggcgagg tcggcgcacc ttgcggcagc
    12421 gagcgcggac ccgtggcgac gaagtacgtc ttgccgtgcg ttgcgacctc ttcgcgcggt
    12481 gactccgtcg cgagcagtgc catgagtgtt gcgacttgct cgccgaggcc tgcgcggcga
    12541 agcagacctt tgacgcggcg ccacgtcacc gtcggataga agccgcggat gtccatcttg
    12601 acgacgatct tcgcgcccgc gtggacgacc gcattcgtca ggatcgagcg accggtgagg
    12661 aagccgtgcg ctgcaccatg aacgggcaag tgctcggtga cttgccgcgt gatccagcgt
    12721 tgcgccgctt tcagatccgg cttgggtgcg gagatgagac gcggtgtgcc gtcgcgcttg
    12781 ggcacgagcc accggtgata gtgcgtgccc gagtcgacgt cgcgatggaa tgccagccag
    12841 cggagcttcg cgaccgtcat gccgagcgct ttcgcaaacg cgtcgacatc gttgatcgcg
    12901 ggcagcgcgt tgtcctgtcg cttcttgtcc ggatcttcgg gatcgaagcg atcgacatcg
    12961 gcggtgtcgt gccaccagac gccgggaccg agatgaacga tgtgcgagcg cttgtatgcg
    13021 gcccacgcct gcttgaacag ctcgcgacgc acgcggcgct cttcttcgcg acgcgccttg
    13081 tagagcttct tctccgcgtc ggacatcgac tgcgacgcgc ccggatcgga gaggccctgc
    13141 ttcgcgactt gggcgtcgac ccattcgcga atgccgccgg cagcggcgac gtcggcccac
    13201 tgtggctgtg cttgctcagc catcgagagc ctctacagaa aaagtggagg aggagtcgaa
    13261 cccgttacca ccctcatggg acacggtagg ctcaccggtc tgcgcggccg gaggggtatt
    13321 ccccgacagc tcaaacctcg gtggcgtggt tggtccagct gagctgtcg
//
LOCUS       DATLBZ010000097        14395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5302580, whole genome shotgun sequence.
ACCESSION   DATLBZ010000097 DATLBZ010000000
VERSION     DATLBZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14395
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5302580"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VMZ53_05130"
     CDS             <1..1088
                     /locus_tag="VMZ53_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS27864.1"
                     /translation="GPGDVYYGDQVRRLASTRWPLQLAPGTSTAVRSKHLQIHLSYDK
                     NVTAAWMSDELSWRIMLCNRTAVIPLRITALYAHDGDRWVQVFEHLSFGRMPQPTFDG
                     SLRGSVMVSPEEDCHRPEFCPVVDRGLSDELSRQLSALLSAQSTRVALTVAGGTRDAT
                     ASSVWDNDPTKPAPTFLLGPDPDGEWDGATSAVETIRLIDGKIVIEGDRRIGTIGIGR
                     TDKATIAYWVGNLTADLTHHPGTPGSKVRLRATFVFEKRKDTWVVASGHISQPIDDID
                     LAQIVFGTALIAEKPLQITCDDGSRSTAQVEPVVPQPQQQPETTPQSKPTPRVKTIKR
                     PAANDPTLVPVQQGSAAPDPAPAPQKK"
     gene            complement(1089..1262)
                     /locus_tag="VMZ53_05135"
     CDS             complement(1089..1262)
                     /locus_tag="VMZ53_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27865.1"
                     /translation="MRKPEPHDLRSLVEKAEAEHHLLARECEARWCDELDARRAALAA
                     ECKRVFERIRTAS"
     gene            complement(1397..2293)
                     /locus_tag="VMZ53_05140"
     CDS             complement(1397..2293)
                     /locus_tag="VMZ53_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUS27866.1"
                     /translation="MLLTREEMTFDFGGAKLDPVRDKELLEWMFNQFLYGEITGIQVG
                     HWLYDAPDLEAAKFLARQSLEEMQHVDNFLRIMTMLGCQPKPAHPAVRFLSTGMMGGS
                     WAEHVALEMAQGEGFVLQAFYAVIDTLDHKPTVDILRRAVKQEERHVEFGEDQTRKSI
                     EAKPWLRRRLLGLSLVSMWGVRKLAGYMEKKLPADHVVLKHLPAFLAHANHCAETRLR
                     RIGVLDRPLAEVSTAKRAALVAEAYGGKVLGSLGSLVTLPLRLLPWFRKKRVTDTYLS
                     DPRVTGYQLPAGNPDAEPQEQL"
     gene            complement(2395..2667)
                     /locus_tag="VMZ53_05145"
     CDS             complement(2395..2667)
                     /locus_tag="VMZ53_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27867.1"
                     /translation="MPRLAGRPDRVFVSVDESTAVSTLSFQPADVGGPVQFQDDKTGA
                     KSMQEARSIIEKYPGCTIHGPHFHTARPPGRMKMRRKPAANTETDE"
     gene            complement(2673..3074)
                     /locus_tag="VMZ53_05150"
     CDS             complement(2673..3074)
                     /locus_tag="VMZ53_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUS27868.1"
                     /translation="MSNRLYVGNLPFHATEDLIQQKFAACGEVREVALMLDRMTGRSR
                     GFCFVEMATSEGAQKALSDLNGQDFEGRPLRVDMAQERPARGGGGGGGGGRGGGGGGW
                     GGGGGGDRGGGYGGGGDRGGGRGGRGGGDRW"
     gene            complement(3190..4500)
                     /locus_tag="VMZ53_05155"
     CDS             complement(3190..4500)
                     /locus_tag="VMZ53_05155"
                     /inference="COORDINATES: protein motif:HMM:NF005557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxyadenosine deaminase"
                     /protein_id="HUS27869.1"
                     /translation="MIFRNGTILTIDPQHRVLAGDVATRDGAIVHIGGSYTPQTSDYS
                     ILDCSGCVIMPGLVQAHVHTCQTLARGRADDLELLDWLRKIIWPYEASLDEAAASASG
                     ELACAELLLGGTTSILDMGTVHHTDAIFAAAERSGIRATIGKAMMDASDPLIPPGLRE
                     STNASLDESARLIDRWHGSAGGRLRYAYAPRFVLSCTDELLREVGVQAKRRGVGIHTH
                     ASENRGEIALVRERFGKDNIVVLDELGLLGPHTCIAHAIHLSDSERALLAARGAHVCT
                     CPSSNLKLASGIAQLPELIAAGVNVALGADGAPCNNNLDGFMELRFVALLHKPRSGPR
                     TLPAPEAVRLATLGGAKALGLGNEVGSLELGKRGDVIAVDVTPLHTIPTASPWSQIAY
                     AAQSCDVRHVAVDGNVVVRDRELLTLEISKVRANGTQAAARLFR"
     gene            4561..5286
                     /locus_tag="VMZ53_05160"
     CDS             4561..5286
                     /locus_tag="VMZ53_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27870.1"
                     /translation="MRRAVVLVVVVGCSNQGQKEGASQPSGAPPQAAPAPTPVKDEWA
                     TPSQPPAAADPWGAEGAQPSQPSPSQPSQPTQPAGSRHAQPAQPAQPAQPEQAAQPET
                     APPAATGDVSALVGHWQINDGYTLQSLRVAADGGVSFEDFTGDGVSQHGCQNKHGTET
                     RGTASVDANILTIRASATTKIYMGCNAPVERTPLAKHDVQFAFAIKNDTLVLTGIDCA
                     NKYGNDVAEIQANCSHKLAHFSD"
     gene            complement(5283..5675)
                     /gene="cdd"
                     /locus_tag="VMZ53_05165"
     CDS             complement(5283..5675)
                     /gene="cdd"
                     /locus_tag="VMZ53_05165"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HUS27871.1"
                     /translation="MSLEDRARAAQKRAYAPYSKFKVGAAIQMKGGEIFEGVNVENAS
                     YPLSVCAERNAIAAAVMGGALELEAVAVCTDASPPSSPCGGCRQVLREFVKDPTKVVV
                     TAVNNKGERRTWTIAELLPDSFSGSELP"
     gene            complement(5672..6532)
                     /locus_tag="VMZ53_05170"
     CDS             complement(5672..6532)
                     /locus_tag="VMZ53_05170"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HUS27872.1"
                     /translation="MTTKRVVETLYDRVQTAAAAVRSRINIVPQVGLILGSGLGAYGD
                     KLDGKASIPYSDVPYFPQSHVVGHKGTLVAGERSGVRCVAMQGRVHMYEGHTAQTVAF
                     PARVLVALGCKVLIITNAAGGMNPRWNPGTLMLIRDHIDMLRDHALRGPNDERLGPRF
                     PDMTKAYAEELRTIVKEVAAAEQIALEEGVYVAMPGPTYETPAEVQMLRRLGADATGM
                     STVPEVVVARHMGARVIGISCITNQAAGISGHELSHAEVTETATRVRSTFERLLDSIL
                     AGLLARGELA"
     gene            complement(6529..7857)
                     /locus_tag="VMZ53_05175"
     CDS             complement(6529..7857)
                     /locus_tag="VMZ53_05175"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HUS27873.1"
                     /translation="MLPVQLIRKKRDGGALDDAELRAFIGWVTDGSIPDYQVAAMLMA
                     IFWRGMTEAELATWADAMTRSGDVIDLSSIKRAKVDKHSTGGVGDKISIPLAPAVAAC
                     GVAVPMVSGRGLGHTGGTLDKLESIPGFKVDLPVEKFAELVDKLGVCLIGQTARIAPA
                     DKRLYALRDVTGTVESLPLIASSIMSKKLAEGIDGLVIDCKVGRGAFMKTVENARALC
                     ALMRAIGQRAGKRVTCVLTDMDAPIGRTIGNSLEIRESIEVLRGGGPVDTRELTSVLG
                     GEMLVLGGVAKDAAEGRAKIDKALADGSALEVFRKVVEAQGGDPRVCDSPGSVLPKPK
                     QREELMLKPGRVVAIDSEALGIAALVLGAGRRTKEDSIDPSAGLVVDAYLGEVIEAGG
                     PPSVMLHHSLSPEDGRVAEARAMIEAAFVIEPADRPASTPRTTRVLEVIR"
     gene            complement(7917..8708)
                     /locus_tag="VMZ53_05180"
     CDS             complement(7917..8708)
                     /locus_tag="VMZ53_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27874.1"
                     /translation="MMRVSALQRGVVGLLACAACGRVNFDLLGGGADDTNPGDDANGG
                     TGDGAMGDGAMGDGSTGGVMVGGSASAFGSGTINTPGTTTLNAGDTIIIATVRGISDT
                     TTVRFAGMDLVENAPIPSFGTGPVFASIWSYRATGQTIGNASSIGNGGSPTAMITVIV
                     HGLPANAFDRSALNAGTGTTATVGPTAITSTATEVVVALAATDVSPCAGSWATGFTEE
                     GPASSTNVCLSVGVKLVTTAATQSATYGAFSSRNWGAAIATYRGP"
     gene            complement(8719..9636)
                     /gene="deoC"
                     /locus_tag="VMZ53_05185"
     CDS             complement(8719..9636)
                     /gene="deoC"
                     /locus_tag="VMZ53_05185"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HUS27875.1"
                     /translation="MSDVDRLVELITQRVKERLSTAPSQVKLNVLPRDRGECNDDSAP
                     GEDCVECGSCVVRRPWSVRAMEGAGAGRVGAAAGVGEVPADLGKLIDHTLLKPEATRD
                     EVVKLCEEARKYRFASVCVNTTWVPLCKSMLAGTDVMVCAVVGFPLGAMTPTAKAYEA
                     REAVRQGAREIDMVINIGALKSRDYETVFEDICRVVKSSAPAGVKVILETSALTQEEK
                     IIAISLSKLAGAAFVKTSTGFGKGGATVEDVQLMRQLVGSELGVKASGGVRTVEDAVK
                     MAQAGANRIGASASVAIVTGKADGKSKGY"
     gene            complement(9679..10011)
                     /locus_tag="VMZ53_05190"
     CDS             complement(9679..10011)
                     /locus_tag="VMZ53_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27876.1"
                     /translation="MMRTIAFALLLTGCSVTFVRDPPNVTPPKGTFVDCTESKALPVL
                     DFVMTGASIGGGIAAVRLLEEYDAPPSHMKAAAVTALFAVPWLLSGLAGMYWTSKCHR
                     MHVEQNRS"
     gene            complement(10080..11624)
                     /locus_tag="VMZ53_05195"
     CDS             complement(10080..11624)
                     /locus_tag="VMZ53_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27877.1"
                     /translation="MPNPTPGTILGAYRVEGQLGAGSMGEVFKGVDTGLNRKVAIKIL
                     SEKHRDSPELRQRFVREGRAVAAISHPNVVQVFATGSFDERPYIAMELLDGTDLGSIV
                     EKQGPLTSVDAAHAILDAAQGLAAAAKAGLIHRDVKPSNLVRLSSSEVKVTDFGLAKP
                     VDPGSEPALTAMGVVVGTPDYIAPEQARGETIDERVDIYALGGTLYFLLTGIPPFRTG
                     KPSEDKYLKVVARHLRNPPPEAASTNPAVDKELSDIAKQMMAKKPLERPSYQELIQRL
                     TGLLARLDPGSVPELIAASRDRSRPNIMPTGVGASLEGGATLSDGELPVAKAGLPAWL
                     IVFSVASVALFVAGLVVYLQRGSDASAEPSKGSAAGSGSAIGTGSGSAQITPIAPAGM
                     ILVKKPDGAPWFFVDAKPVTVSSFKAFFADHKQTTGGPDDSVTDIKYSEAQAYLSGKQ
                     GRLLTAEEYEAAAKSPGFVPTKDVGELLRSDDPSKRLVRTPGGKIETRADTSQKDVTF
                     RMAKDL"
     gene            complement(11678..13582)
                     /locus_tag="VMZ53_05200"
     CDS             complement(11678..13582)
                     /locus_tag="VMZ53_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27878.1"
                     /translation="MRLSVCVFVALVGVAAGRGPAAAQLARYPRQQPPPAPPAASDQL
                     APLVAKGIADLPATTAPSIHADDVMSIDGLRGGIRTEQEQILVELIANTPDSEVEEKS
                     DYYFRLGELYAKQQRFWHDKTIELTAAGNQKEAALAASKAKEFLLKTVKTYKGLTGNE
                     AFRNYPKMDVALFYYAYTLQSGKYMKEARAVYDLLVKTYPSSKYIPEAHLAFADYYAE
                     AGQLADAEERYRKVLEFPKSGVYAYALYRIGWIQLQLQRPQEALTAFANVVRVTKNDR
                     KQEALYRAAKTDFVRASIATGKRERALPAIETLARENTDVLDIYADMAWEQGASEQII
                     AAYRALSTREPADERACVWQYRITRATLSLPKSTTAMKEMTALGRFVSDKVEADPDCR
                     ADTLEMASQLAATYVVDWSKTQNAAKLEDAEQLYAMLVTAAPDDVDARAQYAEVLWAR
                     ADHETNAKLRREKWQRAADVFAAIGTAEAAEAAALGWMNALDIVVPADAKVTLAKSTR
                     KAPRPLPLPAAETKLLTAIASYASQASADDDQLAQMRLAAAITWRKYRHFDEAVAMLD
                     AFLEHHQAHPRAELAANLLLDSMVQRGKPDETFDVVEAIAGDLAFLNGKPELVRNIGL
                     LRARSLHAAR"
     gene            complement(13677..>14395)
                     /locus_tag="VMZ53_05205"
     CDS             complement(13677..>14395)
                     /locus_tag="VMZ53_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27879.1"
                     /translation="VAPGFSGCSYDLMSAASTVDIGVAVTRGTNAFVIRGELLRDAAL
                     DCFRKPAGGTGPLATDDHGVVTVTYPDGRIRVVTFVDDHTLVMEDTKAPAKRDAKELA
                     RHLQEALQAGAPLAKNASYLAAMERMKKQAGVVAVSVPGSEAMAKSMQQMGVKVAFFT
                     GWLDLTDRLQLQYAMELNSADDATQMANMMKGQLSSPSVKQMFDQIDTRAQDKTVTLD
                     LSLNDAKLASLAGMMRGMLL"
BASE COUNT         2393 a         4898 c         4632 g         2472 t
ORIGIN      
        1 tcggtcccgg cgacgtgtac tacggcgatc aagtgcggcg gcttgcgtcg acgcggtggc
       61 cgctgcagct ggcgcccggc acgtcgacgg cggtgcgcag caaacatctt cagattcacc
      121 tgtcgtacga caagaacgtc accgcggcgt ggatgtccga cgagctgtcg tggcggatca
      181 tgctgtgcaa tcgcacggcg gtcatcccgc tgaggatcac ggcgctctac gcgcatgatg
      241 gcgatcgctg ggtgcaggtc ttcgagcacc tgtcgtttgg acggatgcca cagccgacct
      301 tcgatggctc gctgcgtggc tcggtcatgg tgtcgcccga agaagattgc catcggcccg
      361 agttctgtcc cgtcgtcgat cgcggcctct cagacgagct gtcgcgccag ctgtccgcac
      421 tgttgtctgc gcaatcgacg cgcgtcgcgc tcaccgtcgc gggtggaacg cgcgatgcca
      481 cggcttcgtc cgtctgggac aacgatccga cgaagcccgc acccaccttc ttgcttggcc
      541 cggatcccga tggcgaatgg gatggcgcga caagcgcggt cgagacgatc cggctcatcg
      601 acggcaagat cgtcatcgaa ggtgatcgac gcatcggaac gatcggcatc ggtcgaaccg
      661 acaaggcgac gatcgcgtac tgggtcggca acctcacggc ggatctgacg catcacccgg
      721 ggacgccagg cagcaaggtc cggctccgcg cgacattcgt gttcgagaag cgcaaggaca
      781 cgtgggtcgt cgcgtcggga cacatctcgc agccgatcga cgacatcgat ctcgcgcaga
      841 tcgtgttcgg caccgcgctc atcgccgaga agccgctgca gatcacgtgc gacgacggta
      901 gtcgcagcac ggcgcaggtg gagcctgtcg tgccgcagcc tcagcagcag cccgagacaa
      961 cgccacaatc gaagccgacg ccgcgggtga agacgatcaa gcggccggcc gcgaacgatc
     1021 cgacgctcgt gccggtgcaa caaggctccg ctgcgccaga cccagctcca gcgcctcaga
     1081 agaagtagct acgacgcggt gcggatgcgt tcgaacacgc gcttgcattc tgcggcgagc
     1141 gcagcacggc gggcgtccag ttcgtcgcac caacgcgcct cacactcccg ggcgagcagg
     1201 tgatgctcgg cctcagcctt ttccacaagg ctgcggaggt cgtggggctc gggtttacgc
     1261 atgtccttca gaagtgcacc ggtcgtacca cggccaagtc cgcaatgcga cttgtgtcgc
     1321 accggcaact cggtcagccg tggccccaga ttgggcctgt tcgcccgtgt cccgggcccc
     1381 gaaggtgagc cttgcgttag agctgttctt gaggttcagc gtcgggattg ccggcgggga
     1441 gctggtagcc ggtgacgcgg ggatcgctca ggtacgtgtc cgtcacccgc ttcttgcgga
     1501 accacggcag cagccgcagc ggcagcgtga caagtgaacc gaggctgccg aggaccttgc
     1561 cgccgtaggc ttcggcgacg agcgcggcgc gcttcgcggt cgagacttct gcgagcggac
     1621 gatcgagcac gccgatgcgg cgcagccggg tctcggcgca gtggttcgcg tgcgcgagga
     1681 atgcgggcag gtgcttcagc acgacgtggt ctgcgggcag cttcttctcc atgtagcccg
     1741 cgagctttcg cacgccccac atcgagacga gcgagaggcc gagcagacgg cgccgcagcc
     1801 acggctttgc ctcgatcgat ttcctggtct ggtcctcgcc gaactcgacg tggcgctctt
     1861 cttgtttgac cgcgcgacga aggatatcga ccgtgggttt gtgatcgagc gtgtcgatca
     1921 cggcgtagaa cgcctggagc acgaagcctt cgccctgcgc catctcgagc gcgacgtgct
     1981 cggcccacga gccgcccatc atgccggtcg acaggaagcg gaccgcagga tgcgcgggct
     2041 tgggctggca gccgagcatc gtcatgatgc gcaggaagtt gtcgacgtgc tgcatctcct
     2101 cgagcgactg gcgcgcgagg aacttcgccg cttcgagatc cggcgcgtcg tacagccagt
     2161 ggccgacttg aatgcccgtg atctcgccgt agaggaattg gttgaacatc cactcgagga
     2221 gctctttgtc tctgacggga tcgagcttcg cgccgccaaa gtcgaacgtc atctcctcgc
     2281 gagtcagcag catcgggtac ctgggcgcag gatagcgcgc gtcgccaggc cccgaaacga
     2341 caaaacccgc gtccctcgga agagaaacgc gggcttgtgc ttacttggac gggtttactc
     2401 gtcggtttcg gtattcgctg ctggcttgcg gcgcatcttc atccgtcccg gaggccgggc
     2461 cgtgtggaaa tgcggaccgt gaatcgtgca gccgggatac ttctcgatga tggagcgtgc
     2521 ttcctgcatg ctcttcgcac cggtcttgtc atcctgaaac tgtacgggcc ctccgacgtc
     2581 tgccggttga aacgacaggg tcgagaccgc cgtgctctcg tctacagaga cgaacacgcg
     2641 atccggtcgg cccgcgagcc gaggcatcga gctcaccagc gatcaccgcc gccgcgacca
     2701 ccacgaccac caccgcggtc gccaccacca ccgtagccac cgccgcggtc gccaccacca
     2761 ccaccgcccc aaccaccgcc accgccgccg cgaccaccgc cgccgccgcc gccaccgccg
     2821 cgagccggac gctcttgcgc catatcgacg cggagaggac gaccctcgaa gtcttggcca
     2881 ttgagatcgg agagcgcctt ctgagcgcct tcggacgtcg ccatctcgac gaagcagaag
     2941 ccgcgcgaac gaccggtcat ccgatcgagc atcagagcga cttcgcgaac ttcaccgcaa
     3001 gcggcaaact tctgctgaat gagatcttcg gtggcgtgaa acggaaggtt accaacgtac
     3061 aaacgattcg acatttcgga aatttctctc tcgcggctcg aacattcgag acaccgcctt
     3121 cagccgctcg gaaggcgcgt ctttggatcg ccctcaacga gaacgcgggg cctgtgtagc
     3181 acgggcaaat tatcggaaca accgcgcagc ggcctgggtg ccgttagccc gcactttgct
     3241 gatttctagg gttaataact cccgatcgcg caccacgacg ttgccgtcca cagcgacgtg
     3301 gcggacgtcg caggactgcg ctgcataggc aatttgcgac cacgggctcg ccgtcgggat
     3361 cgtatggagc ggcgtcacat cgactgcaat gacatcgccg cgcttgccga gctcgagcga
     3421 gccaacttcg ttgccaaggc cgagcgcttt tgcaccaccg agtgtggcaa ggcggaccgc
     3481 ctcgggcgcg ggcagcgtcc gtggcccgct tcgcggcttg tgcaggagcg cgacaaaccg
     3541 cagctccatg aagccatcga ggttgttgtt acacggcgcg ccatcggcgc cgagcgcgac
     3601 attgacgccc gcggcgatga gctcgggaag ctgcgcgatg cccgacgcga gcttgaggtt
     3661 gctcgatggg cacgtgcaga cgtgggcacc tcgcgcggcg agcagcgcgc gctcggaatc
     3721 ggagagatgg atcgcgtgcg cgatgcacgt atgcgggccg agcaggccga gctcgtcgag
     3781 gacgacgatg ttgtccttcc caaacctctc gcgaacgagg gcgatctcgc cgcggttctc
     3841 gctcgcgtgc gtgtgaatgc cgacgccgcg gcgcttcgcc tgcacgccga cctcgcgcag
     3901 caactcgtcc gtgcacgaca ggacgaaccg cggcgcatag gcatagcgca gccggccgcc
     3961 agcagacccg tgccagcgat cgatcaagcg cgcggattca tcgagcgatg cgttcgtcga
     4021 ctcgcgcagc ccgggcggga tgagcggatc gctcgcgtcc atcatcgcct tgccgatcgt
     4081 cgcgcgaatg ccgctgcgct ccgccgccgc aaagatcgca tcggtgtggt gcaccgtgcc
     4141 catgtcgagg atcgacgtcg tgccgccgag caagagctcc gcacacgcga gctcgccgga
     4201 tgcgctcgca gcggcttcgt cgagcgacgc ctcgtacggc cagatgatct tgcgcagcca
     4261 atcgagcagc tcgagatcat cggcgcggcc gcgcgcgagc gtctggcacg tgtgcacgtg
     4321 cgcctgcacg aggccgggca tgatcacgca gccgctgcag tcgaggatcg aatagtcgct
     4381 ggtctgcggc gtgtagctgc cgccgatatg aacgatggca ccgtcgcgcg tcgcgacatc
     4441 accggcgagc acgcggtgct gcggatcgat cgtgaggatc gtcccgttgc ggaaaatcac
     4501 ctcgctcaac gggagcaatt atcgcccatc cgggtcgcgg gttggggcat cgtacgcacc
     4561 atgcgtaggg ctgtcgtgct ggtcgtcgtc gtcggttgct cgaatcaggg acagaaggaa
     4621 ggcgcgtcgc aaccgtcggg cgcgccgccg caagccgccc ccgcaccgac gccggtgaaa
     4681 gacgaatggg cgacgccatc gcaaccgcca gcggctgcgg atccgtgggg cgcagagggt
     4741 gcccagcctt cgcagccgtc gccgtcacag ccctcgcaac caacgcaacc ggcgggctcg
     4801 cggcacgcgc aaccggcgca accggcgcaa ccggcgcaac cggagcaggc cgcacagcca
     4861 gagaccgctc caccggccgc gaccggcgat gtgtcggccc tcgtcggtca ttggcagatc
     4921 aacgatggct acacgctgca gagcctgcgc gtcgccgcgg atggtggcgt gtcattcgaa
     4981 gacttcaccg gcgatggggt gagccagcac ggctgccaga acaagcatgg caccgagacg
     5041 cgtggcacgg cgtccgtcga cgcgaacatc ctgacgatcc gcgcgagcgc gacgacgaag
     5101 atctacatgg gctgcaacgc acctgtcgag cgcacgccgc tagccaagca cgacgtgcag
     5161 ttcgcattcg cgatcaagaa cgacacgctc gtgctgacgg gcatcgactg cgcgaacaag
     5221 tacggcaacg acgtcgccga gatccaagcg aactgctcgc acaagctcgc gcacttctcg
     5281 gactaaggca gctcggagcc ggagaaggaa tcgggcaaca gctcggcgat cgtccaggtg
     5341 cggcgctcac ctttgttgtt caccgccgtg acgacgacct tcgtcggatc tttcacgaac
     5401 tcgcgcagca cttggcggca cccgccgcag ggcgacgacg gcggcgaagc atcggtgcag
     5461 accgcgaccg cttcgagctc gagcgcgccg cccatcactg ctgcagcgat cgcgttgcgc
     5521 tccgcgcaga ccgacagtgg gtacgaagcg ttctccacgt tgacgccctc gaagatctcg
     5581 ccgcccttca tctggatcgc ggcgccgacc ttgaacttcg agtacggcgc atacgcgcgc
     5641 ttctgggctg cgcgcgctcg gtcttcgagg ctcaagcgag ctctccgcga gcgagcagac
     5701 ccgcgaggat cgaatcgagc aagcgctcga acgtcgagcg cacgcgcgtc gcggtctcgg
     5761 tgacctcggc gtgcgagagc tcgtgaccgc tgatgcccgc cgcttggttc gtgatgcacg
     5821 agatgccgat cacgcgtgcg cccatgtgac gcgcgacgac gacttcgggc accgtcgaca
     5881 tgccggtcgc gtccgcgccg agccggcgga gcatctggac ttcggcgggc gtttcgtacg
     5941 tcggtcctgg catcgcgacg tagacgcctt cttcgagcgc gatctgttcg gcggccgcga
     6001 cctccttgac gatcgtgcgc agctcttcgg cgtacgcctt cgtcatgtcg gggaagcgag
     6061 ggccgagtcg ctcgtcgttg gggccacgca gcgcgtggtc gcgcagcatg tcgatgtgat
     6121 cgcggatcag catcagcgtg ccgggattcc agcgcgggtt catgccgccg gcggcgttcg
     6181 tgatgatgag gaccttgcag ccgagcgcga cgagcacgcg cgccggaaac gcgacggtct
     6241 gcgcggtgtg gccttcgtac atgtgcacgc ggccctgcat cgcgacgcag cgcacgcccg
     6301 agcgttcacc ggcgacgagc gtgcccttgt ggccgacgac gtgcgactgc gggaagtacg
     6361 gtacgtcgga gtaggggatc gaggccttgc cgtcgagctt gtcgccgtag gcgccgaggc
     6421 ccgagccgag gatgaggccg acttgcggca cgatgttgat gcgactgcgc accgcggcgg
     6481 ccgccgtttg cacgcggtcg tagagcgtct cgacgacgcg cttcgtcgtc atcggatcac
     6541 ctcgaggacg cgcgtcgtgc gcggcgtgga ggcgggtctg tcggcaggct cgatcacgaa
     6601 ggcggcttcg atcatcgcac gcgcctcggc gaccctgcca tcttccggcg acaagctgtg
     6661 gtgcagcatg acgctaggcg gcccacccgc ctcgatgacc tcgccgagat aggcgtcgac
     6721 gacgaggcct gccgacggat cgatcgagtc ttctttcgta cggcgtcccg cgccgagcac
     6781 gagggccgcg atgccgagcg cctcgctgtc gatcgcgacg acgcggccgg gcttcagcat
     6841 caactcttcg cgctgcttgg gcttcggcag cacgctgccc ggtgagtcgc acacgcgcgg
     6901 atcgccgccc tgcgcttcga cgaccttgcg gaagacttcg agcgcgctgc cgtctgcgag
     6961 cgccttgtcg atcttcgcgc ggccttccgc ggcatccttc gcgacgccgc cgagcacgag
     7021 catctcgccg ccgagcacgc tcgtcagctc ccgcgtatcg accgggccgc cgccgcgcag
     7081 cacttcgatc gattcgcgaa tctcgagcga gttgccgatc gtgcggccga tcggagcatc
     7141 catatccgtg agcacgcacg tcacgcgttt gccggcgcgc tggccgatcg cacgcatgag
     7201 cgcgcacagt gcacgcgcat tctcgaccgt cttcatgaac gcgccgcggc cgaccttgca
     7261 gtcgatcacg aggccgtcga taccctccgc gagcttcttg ctcatgatcg acgacgcgat
     7321 cagcggcagt gattcgacgg tgcccgtgac gtcgcggagc gcatagaggc gcttgtcggc
     7381 aggtgcgatg cgcgcggtct ggccgatgag gcagacaccg agcttgtcga cgagctccgc
     7441 gaacttctcg acgggcaagt cgaccttgaa gccggggatg ctctcgagct tgtcgagcgt
     7501 cccgccggtg tggccaagcc cgcggcccga taccatcggc acggcgacgc cacacgccgc
     7561 cacggcagga gcgagcggga ttgaaatctt gtcgccgaca ccgccggtcg agtgcttgtc
     7621 caccttcgcc cgcttgatcg acgacagatc gatcacgtcg cccgagcgag tcatcgcatc
     7681 ggcccacgtc gcgagctccg cctcggtcat gccgcgccag aagatcgcca tcagcatcgc
     7741 cgcgacttgg tagtccggga tgctgccgtc cgtgacccac ccgatgaacg cgcggagctc
     7801 ggcgtcgtcg agcgcgccgc catcgcgctt cttgcggatc aactgaacgg gaagcacgtc
     7861 gctgattctg gcaacgcgct gagatcattg gaagctcccg cggcaagttc cgcgtttcag
     7921 gggccgcgat acgtcgcgat cgctgcgccc caattgcgcg aggaaaacgc cccgtacgtc
     7981 gccgactggg tggctgccgt cgtcacgagc ttcacgccga ccgacagaca gacatttgtc
     8041 gagctcgccg gaccttcctc cgtgaatccg gtcgcccacg accccgcgca cggcgacaca
     8101 tcggtggcgg ccagggcgac gacgacctcc gtggcggtgg aggtgatcgc cgtcgggccc
     8161 accgtcgcgg tcgtgccggt gcctgcattg agcgccgagc gatcgaatgc attcgccggc
     8221 agcccgtgca cgatgaccgt gatcatcgca gtgggagagc cgccgttgcc gatcgagctc
     8281 gcattgccga tcgtctgccc ggtcgcgcga taggaccaaa tggaggcgaa caccggtccc
     8341 gtcccgaacg atggaatggg cgcgttctcg acgagatcca tgcccgcgaa tcgcaccgtc
     8401 gtcgtgtcgg agatgccgcg cacggtcgcg atgatgatcg tgtcgccggc attcagcgtc
     8461 gtcgtgccgg gcgtgttgat cgtcccgctt ccgaacgcag atgcgcttcc gccgaccatc
     8521 acgcctccgg tcgatccgtc gcccatcgcg ccgtcgccca tcgcgccgtc accggtgccg
     8581 ccgttcgcgt cgtcgcccgg gttcgtatcg tcggcaccgc cgccgagcag atcgaaattc
     8641 acgcggccgc acgccgcaca cgccaggaga ccgaccacgc cgcgttgaag ggcggaaacg
     8701 cgcatcaccg cgccgaggct agtagccctt cgacttgccg tccgctttac cagtcacgat
     8761 cgcgaccgac gcgctcgcgc cgatgcgatt cgcgccggcc tgcgccatct tcacggcgtc
     8821 ctcgacggtg cgcacgccgc ccgatgcttt gacgccgagc tccgagccga cgagctgccg
     8881 catgagctgc acgtcctcga cggtggcgcc gcctttgcca aatccagtcg acgtcttcac
     8941 gaatgccgcg cccgcgagct tcgagaggga gatcgcgatg atcttttcct cctgcgtgag
     9001 cgcgctcgtc tcgaggatta ccttcacgcc ggcgggcgcc gacgacttga cgacgcgaca
     9061 gatgtcctcg aacaccgtct cgtaatcgcg cgacttcagc gcgccgatgt tgatgaccat
     9121 gtcgatctct cgcgcgcctt ggcggacggc ttcgcgcgct tcgtaggcct tcgccgtcgg
     9181 cgtcatcgcg ccgagtggaa agccgacgac cgcacacacc atcacgtcgg tgcccgcgag
     9241 catcgacttg cagagcggca cccacgtcgt gttcacgcac accgatgcga accgatactt
     9301 gcgagcttcc tcgcagagct tgacgacttc gtcgcgcgtg gcttcgggct tcagaagtgt
     9361 gtgatcgatc agcttgccga gatccgcggg cacttcgccg acgcccgctg ctgcaccgac
     9421 gcgacccgcg ccggcacctt ccatcgcgcg caccgaccac gggcgccgca cgacgcacga
     9481 gccgcactcg acgcagtctt cgccgggcgc gctgtcgtca ttgcactcgc cgcgatcgcg
     9541 cggaagcacg ttgagcttca cctgcgaagg cgcagtcgac aggcgttctt tcacccgctg
     9601 ggtgatcagc tcgacgaggc ggtcgacatc cgacatcgcc cgaaccatac ctcggttcgc
     9661 ttcagcgcgc tttcgcggtc aggagcgatt ctgctcgacg tgcatgcggt ggcacttcga
     9721 cgtccagtac atcccggcga gccccgacag cagccacggc actgcgaaca acgcggtcac
     9781 ggcagcagcc ttcatgtgac tcggcggcgc gtcgtactcc tcgagtaagc gaacggctgc
     9841 gatgccaccg ccgatgctgg cgcctgtcat cacgaagtcc agcacgggca gcgccttcga
     9901 ttcggtgcaa tcgacgaacg ttcccttcgg cggcgtgacg ttcggcggat ctctcacgaa
     9961 cgtgaccgag catcccgtca acagcaacgc aaacgcgatc gtgcgcatca tcgagtcccc
    10021 gcggacgacg ccatctcacc agcctacgcg accccgcgtg ccagacgcga cgattcaggt
    10081 cacaggtctt tggccatgcg gaacgtgacg tctttttgcg acgtgtcggc gcgcgtctcg
    10141 atcttgccac ctggcgtgcg cacgaggcgc ttcgatggat cgtcggagcg aagcagctcg
    10201 ccgacgtctt tcgtcggcac gaagcccggc gacttcgcgg ctgcttcgta ttcctcggcg
    10261 gtcagcaggc gtccttgctt gccagagagg taggcctgcg cctcgctgta cttgatgtcg
    10321 gtgacagaat cgtcggggcc gccggtcgtc tgcttgtgat cggcgaagaa ggccttgaac
    10381 gacgagacgg taacgggctt cgcgtcgacg aagaaccacg gtgcgccgtc gggcttcttc
    10441 acgagaatca tgccggcagg tgcgatcggc gtgatctgcg cggagccgct ccccgtaccg
    10501 atcgccgagc cgctgcctgc ggccgagccc ttcgacggct ccgcggacgc atcactgccg
    10561 cgctgcagat acacgacgag gcccgcgacg aacagcgcga cgctcgcgac ggagaacacg
    10621 atcagccacg cgggcaggcc tgccttcgcg acaggcaact caccatcgga gagcgttgcg
    10681 ccgccttcga gcgatgcgcc cacgcccgtc ggcatgatgt tcggccggct gcgatcgcgc
    10741 gatgccgcga tgagctcggg cacgctgccc ggatcgaggc gcgcgagcag gccggtcagg
    10801 cgctggatca gctcttgata gctcggccgc tccagcggct tcttcgccat catctgcttc
    10861 gcgatatccg acagctcttt gtcgacggcg ggattcgtgc tcgccgcttc gggcggcggg
    10921 ttgcgcaggt gacgtgcgac gaccttgaga tacttgtctt cgctcggctt gcccgtgcgg
    10981 aacggcggga tgcccgtgag caagaagtag agcgtgccgc cgagcgcgta gatatcgact
    11041 cgctcgtcga tcgtctcgcc acgcgcttgc tcgggcgcga tgtaatccgg cgtgccgacg
    11101 acaacgccca tcgcggtcag cgcgggctcg cttcccggat cgacgggctt tgcgaggccg
    11161 aagtcggtga ccttcacctc gctgctcgag aggcgcacga ggttcgacgg cttgacgtcg
    11221 cgatggatga ggccggcctt cgccgcagcg gccaagccct gcgcagcgtc gaggatcgcg
    11281 tgcgccgcat cgaccgatgt cagcggacct tgcttctcga cgatggagcc caagtcggtg
    11341 ccgtcgagga gctccatcgc gatgtacggc cgctcgtcga agctgccggt cgcgaacacc
    11401 tgcacgacat tcggatggct gatcgccgca acggcgcgtc cctcgcgcac gaaccgctgg
    11461 cgcagctcgg ggctgtcgcg atgcttctcg ctcaggatct tgatcgcgac tttgcgattg
    11521 aggcccgtgt cgacgccctt gaagacctcg cccatcgagc cggcaccgag ctggccctca
    11581 acgcgatacg cgcccaggat ggtccccggc gtagggttcg gcacggcgcc atcttacgat
    11641 gacctttggg gctagaggca aggccgcgtc agcgaactca ccgggcggcg tggagcgagc
    11701 gcgcccgaag aagccctatg ttgcgcacga gctccggctt gccgttcaga aacgcgagat
    11761 cgcccgcgat cgcttcgacg acgtcgaacg tctcgtcggg cttgccgcgc tggaccatcg
    11821 aatcgagcaa cagattcgca gcgagctctg cgcggggatg cgcctggtga tgctcgagga
    11881 acgcgtccag catcgcgacg gcttcatcga agtggcgata cttccgccac gtgatcgcag
    11941 cggcgaggcg catctgcgcg agctggtcgt cgtcggccga agcctggctc gcgtagctcg
    12001 cgatcgcggt gagcagcttc gtttcggctg cgggcaacgg cagcggccgc ggtgccttgc
    12061 gcgtcgactt cgcgagcgtg accttcgcat ccgccgggac cacgatgtcg agtgcgttca
    12121 tccagccgag cgccgcagcc tccgctgctt cggccgttcc gatcgcagca aacacgtcgg
    12181 cagcgcgctg ccatttctcg cgccggagct tcgcattcgt ctcgtgatcg gcgcgcgccc
    12241 acagcacctc cgcgtattgc gcgcgggcat cgacatcgtc gggcgccgcg gtgacaagca
    12301 tcgcgtagag ctgctccgcg tcctcgagct tcgcggcatt ctgcgtcttg gaccagtcga
    12361 ccacgtacgt cgcagcgagc tgactcgcca tctcgagcgt gtccgcgcgg cagtcggggt
    12421 cggcctccac cttgtcggag acaaaccgcc cgagtgccgt catctctttc atcgccgtcg
    12481 tgctcttcgg aagtgacaac gtcgcgcgcg tgatgcggta ctgccagacg catgctcgtt
    12541 catcggccgg ctcacgcgtc gagagcgcgc gatacgccgc gatgatctgc tcggacgcgc
    12601 cttgctccca cgccatgtcg gcgtagatgt cgagcacgtc cgtgttctct cgcgccagcg
    12661 tctcgatcgc aggcaatgcg cgctcgcgct tccccgtcgc gatcgatgcg cgcacgaagt
    12721 cggtcttcgc tgcgcgataa agcgcctctt gcttgcggtc attcttcgtc acgcggacga
    12781 cattcgcgaa cgcggtgagt gcttcctgtg gccgctgtag ctgcagctga atccagccga
    12841 tgcggtacag cgcgtacgcg tacacacccg acttcggaaa ctcgagcacc ttcctatagc
    12901 gttcctcggc atcggcgagc tgcccggcct cggcgtagta gtccgcgaac gcgaggtgcg
    12961 cttcgggaat gtacttagag ctcggatacg tcttgacgag caggtcgtag acggcgcgcg
    13021 cctccttcat gtacttgccg ctctgcagcg tgtacgcgta ataaaacagc gcgacatcca
    13081 tcttcggata attgcgaaag gcctcgttgc cggtgagccc cttgtaggtc ttcaccgtct
    13141 tcagcaaaaa ctccttcgcc ttgctcgcag cgagggccgc ttccttctgg ttgcccgccg
    13201 ccgtcagctc gatcgtcttg tcgtgccaga accgctgctg cttcgcgtag agctcgccaa
    13261 gccgaaagta gtaatcggac ttctcttcga cctcgctatc gggcgtgttg gcgatgagct
    13321 cgacgaggat ctgttcctgc tcggttcgaa tgccgccacg caacccatcg atcgacatca
    13381 cgtcatcggc atggatcgac ggcgccgtcg tcgccggcag atcggcgatt cccttcgcaa
    13441 caagcggtgc tagctggtcc gacgcagcgg gcggcgccgg cggtggctgc tgccgcgggt
    13501 accgcgcgag ctgcgcggct gcaggcccac gtccagcggc aactcccacg agcgcgacga
    13561 agacgcaaac cgagagcctc atggtctcgc tacatacgca cgagcccgcc gttcgatcca
    13621 atggcggtca ctggcccggt gtcgacgcgc cgcctcgtcg ctgtcgcgat ctgcgattac
    13681 agcaacatgc cgcgcatcat ccccgccaga ctcgcgagct tcgcatcgtt caacgacagg
    13741 tcgagcgtca cggtcttgtc ctgcgcacgc gtgtcgatct gatcgaacat ctgcttcacg
    13801 gacggcgacg acagctgccc cttcatcatg ttcgccatct gcgtcgcgtc gtcggcgcta
    13861 ttgagctcca tcgcatattg cagctgcagg cgatcggtca gatcgagcca gcccgtgaag
    13921 aacgccacct tcacgcccat ctgctgcatc gacttcgcca tcgcttcgct gccggggacc
    13981 gacacggcga cgacaccagc ctgcttcttc attcgctcca tcgcggcgag gtacgacgcg
    14041 ttcttcgcga gtggcgcgcc cgcttgcagc gcctcctgca ggtgcctcgc gagctccttc
    14101 gcgtcgcgct tcgccggcgc cttcgtgtcc tccatgacga gcgtgtgatc gtcgacaaat
    14161 gtgaccactc gaatccggcc gtccggatac gtcaccgtca cgacgccatg gtcgtccgtc
    14221 gccaacggcc cggtaccacc agcgggcttc cgaaagcagt cgagcgccgc atcgcgcagc
    14281 agctcgccgc gaatgacgaa ggcattcgtg ccgcgcgtga ccgcaacgcc gatgtcgacg
    14341 gtcgacgctg cgctcatcag atcgtacgaa cagccggaaa atccaggcgc gacga
//
LOCUS       DATLBZ010000098         3561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9733727, whole genome shotgun sequence.
ACCESSION   DATLBZ010000098 DATLBZ010000000
VERSION     DATLBZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9733727"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VMZ53_05210"
     CDS             <1..359
                     /locus_tag="VMZ53_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27880.1"
                     /translation="HHDGTVSIAGQAPDALVFKRNGHVIGLPQPVAKEPFDVRPATPD
                     NELRARTPESQRGGTAPTPSKFAQTETVVLAKAALTQAGFRAAIAKRAVELACAHVGT
                     NADLTQLIKEALRHCG"
     gene            complement(884..1516)
                     /locus_tag="VMZ53_05215"
     CDS             complement(884..1516)
                     /locus_tag="VMZ53_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27881.1"
                     /translation="MRWAFPLLLLAGCAGVRSDFPTIQLDCATGTNCEGKLGVGGKVY
                     ASREVLSHNEDVLHAEASDLHGLVGVAPGTAEISLIDRDMVVDSAEIEVAPLTMLRLA
                     PIASTVIDGPLLDATYNASYGVTASTPINVKLTAEINGARSIGSHFYIVFLDGERYDC
                     YGGYSNVPRICTGPVWYDTLPIDPLTPGDHVLELQSIDGGRDFSFRLAAR"
     gene            1643..>3561
                     /gene="mutS"
                     /locus_tag="VMZ53_05220"
     CDS             1643..>3561
                     /gene="mutS"
                     /locus_tag="VMZ53_05220"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HUS27882.1"
                     /translation="MGRAPADTPLMRQYLDLKEKHPDALLFFRCGDFYELFFEDAVIT
                     ARALDLVLTSRDKGKEDSVPMCGVPYHAVRGYLARLTEQGHKIAICEQMEDPKLAKGL
                     VKRDVVRVITPGVVLDDDILDPKLPRYLAAVVPGGKDASVGLAYLDATTGELYATELQ
                     ASAVLDELVRVAPREVLADGGLLAKETGIVAQLRMRYRVPWNPVVVPGIEAAEKVVAA
                     IGGAGSGAAASRPASSSEGQLAATRASSSSTRDGASVGASLSPTLDDRPLAIRAAASV
                     LAYARATQPTGTLPITQVKAYEPGDAVVLDETAIANLELVETLIGRRREGSLLDVIDQ
                     TVTAPGARLLRRWMLYPLVDVAAIRRRQDGVGWLVERPALLEQIRRALGKIADLERLA
                     GKATLGVATPRDLGRLRDALDQLPELVSLVRSGATKPSATIDPLPELLDLTAATDKAL
                     PALATRLGKALVDEPPYLLKDGGVIRAKYDGTVDECRRLADGGKDEILAIEVREQKES
                     GIANLKVRYNRVFGYYIEITKTHLAKVPSHYIRKQTIATGERYVTTELAELEKKVLTA
                     EETLLAREAELFRQEVLAVAEAAPKISAAGAALAVLDACASLAEVAAKRSYCRPVVDD
                     GLALELGDARHPVIEA"
BASE COUNT          599 a         1130 c         1277 g          555 t
ORIGIN      
        1 tgcaccacga cgggacggtc tcgatcgcag gtcaggcacc ggacgccctc gtgttcaaac
       61 gtaacggtca cgtcatcgga ctgccgcagc cagtcgcgaa agagccgttc gacgttcgac
      121 cagcgacgcc ggacaacgag ctgcgcgcgc gcacccccga gtcgcagcgc ggcggcaccg
      181 caccgacgcc ctcgaagttc gcgcaaaccg aaacggtggt gctcgcgaag gccgcgctga
      241 cccaggcggg cttccgagcc gcgatcgcca agcgggcagt cgagcttgcc tgtgcccacg
      301 tgggcacgaa cgccgaccta actcagctca ttaaagaggc tctgcgccac tgcggctaat
      361 gcggagtccg aaacccgcgg ttcgtgccgc gcgttcgggt aaccgcggtt gtcgtgccca
      421 cgtgggcacg agtcgcacgc gtgggccgag tgctgatcat cgagcccccg acatcgtcgc
      481 gcggcagcgc acgcgatgcc cgaacggggc gacctacgac ctcgcgatga aggccttcgt
      541 caacgacgtg caggtcacga tgcagaggat cagaagcgat cagcaatgtg cgagccgcgc
      601 gggccggcgg cgacgtcgcg gcgcgcgccc ctggccgttt gaaaggccgg ggcgcgccgc
      661 tcccccgccg gcccggcgct ccctgccgag tgagggacag agacctcgcc gagcgcaagc
      721 gtggttcgcc gtacccacgt gcgcacgaat cttgatccca ccgcgagctg cgcgagagcc
      781 gtcggcgcgc ctgcgcacga cctgccgcta cagcccgcgg atcgcccccg acgcccacga
      841 ggttatcgtg cccacgtggg cacgaacgcc ggtcatcgag acattagcgc gcggcgaggc
      901 ggaagctgaa gtcgcggcca ccgtcgatcg attgcagctc gagcacgtgg tcaccgggcg
      961 tcagcggatc gatcggcagc gtgtcgtacc agacgggacc ggtgcagatg cgcggcacgt
     1021 tgctgtagcc gccgtagcag tcgtagcgct cgccatcgag gaagacgatg tagaagtgcg
     1081 agccgatcga gcgtgcgccg ttgatctcgg cggtgagctt gacgttgatg ggtgtgctcg
     1141 ccgtgacgcc gtacgacgcg ttgtatgtag cgtcgaggag tgggccatcg atgaccgtgg
     1201 atgcgatcgg cgctaggcgc agcatcgtga gcggcgcgac ctcgatctcg gcgctgtcga
     1261 cgaccatgtc gcgatcgatc agcgagatct cggcggttcc tggcgcgacg ccgacgagac
     1321 cgtggaggtc gctggcctcg gcgtggagga cgtcctcgtt gtggctgagg acctcgcgag
     1381 acgcgtagac cttgccgccg acgccgagct tgccttcgca gttcgtgccg gtggcgcagt
     1441 cgagctgaat cgtcgggaaa tcggaccgca cgccagcaca gccggccagc agcagaagag
     1501 ggaatgccca gcgcatcgtc gtcacagggg acataacccg cgagcgtcgc taggtgtgca
     1561 gaatatttcg ggcagcgacg gtaggtgcgc ggaatgagcg tggttaggca gcgatgtcgg
     1621 aggggcgtgc tacctgcgtt cggtgggtcg cgcgcctgcc gatacgccgc tgatgcggca
     1681 atacctcgat ctaaaggaga agcatcctga tgcgttgctc ttctttcggt gcggcgattt
     1741 ctacgagctg tttttcgaag acgcggtgat cacggcgcgc gcgctcgacc tcgtgctcac
     1801 gtctcgcgac aagggcaaag aggacagcgt cccgatgtgc ggcgtgccgt atcacgcggt
     1861 ccgcggctat ctcgcgcggt tgaccgagca ggggcacaag atcgccatct gcgagcagat
     1921 ggaggatccg aagctcgcga agggcttggt caagcgcgac gtcgttcgcg tgatcacgcc
     1981 gggcgtcgtg ctcgacgacg acatcttgga tcccaagctg ccgcggtatt tggcggcggt
     2041 ggtgcctggc ggaaaagatg cgagcgtcgg gctcgcgtat ctggacgcga cgacgggcga
     2101 gttgtacgcg acggagctgc aggcaagcgc cgtcctcgac gagcttgttc gtgtcgcgcc
     2161 gcgcgaggtg ttggcggatg gcgggttgct cgcgaaggag acgggcatcg tcgcgcagct
     2221 gcgaatgcgt tatcgcgtgc cgtggaatcc ggtcgtcgtg ccggggatcg aagcggcgga
     2281 gaaggttgtc gcggcgatcg gcggggcagg gagcggtgcg gcagcgtcgc ggcccgcgtc
     2341 gtcgagcgag gggcagttag cggcgacgcg tgcttcgtcg agtagcacgc gtgatggcgc
     2401 gagcgtcggg gcgagcttgt cgccgacact cgatgatcgg ccgttggcaa ttcgtgccgc
     2461 ggcgagtgtg cttgcgtatg cgcgcgcgac gcagccgacc ggcacgttgc cgatcacgca
     2521 ggtgaaggcg tacgagcccg gcgatgcagt cgtcctcgac gagacggcga tcgcgaatct
     2581 ggagctcgtc gagacgctca tcgggcggcg gcgcgaaggc tcgctgctcg acgtgatcga
     2641 tcagacggtc acggcgcctg gcgcgcggct gttgcggcgc tggatgctgt atccgctcgt
     2701 cgatgtcgcg gcgatccggc ggcggcaaga cggcgtcggc tggctcgtcg agcggccggc
     2761 gttgctcgaa cagattcgca gagcgctcgg caagatcgcc gacctcgagc ggctcgcggg
     2821 caaggcgacg ctcggcgttg cgacaccgcg cgatctaggc cggctgcgcg acgcgctcga
     2881 tcagctgccc gagctcgtct cgctcgtgcg ctcgggcgcg acgaagccaa gcgcgacgat
     2941 cgacccgttg ccggagctcc tcgatctcac ggccgcgacc gacaaggcgc tgcccgcgct
     3001 cgccacgcgc cttggcaagg cgctcgtcga cgagccgcca tatttgctca aagacggcgg
     3061 cgtcatccgt gcgaagtacg acggcaccgt cgacgaatgc cggcgactcg ccgatggcgg
     3121 caaggacgag atcctcgcga tcgaagtgcg cgagcagaag gagagcggca tcgcgaatct
     3181 caaggttcgc tacaaccgcg tctttggtta ctacatcgag atcacgaaga cgcacctcgc
     3241 caaagttccg tcgcactaca ttcgcaagca gacgatcgcg acgggcgagc gctacgtgac
     3301 gaccgagctc gccgagctcg agaagaaagt gctcaccgcg gaggagactc tgctcgcgcg
     3361 cgaggccgag ctgttccgcc aagaggtgct cgccgtcgcc gaagcggcgc cgaagatttc
     3421 agcggcaggt gcggcgctcg ccgtcctcga tgcgtgcgcc tcgctcgcgg aggtcgcggc
     3481 caagcgcagc tactgccggc cggtcgtcga cgatggcctg gcgctcgagc tcggcgacgc
     3541 gcggcatccc gtgatcgaag c
//
LOCUS       DATLBZ010000099         3784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4558299, whole genome shotgun sequence.
ACCESSION   DATLBZ010000099 DATLBZ010000000
VERSION     DATLBZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4558299"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..421
                     /locus_tag="VMZ53_05225"
     CDS             107..421
                     /locus_tag="VMZ53_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27883.1"
                     /translation="MAGPTETVLKARLLPSVAHPRAVPTLLEALALWQGSTVRAALVV
                     DDVDGSSGTRLGLDSWVGLAGPPLYEVEFVPRRKPHHRDALDGMGAFHDLRQLTMFEV
                     AR"
     gene            418..1926
                     /gene="istA"
                     /locus_tag="VMZ53_05230"
     CDS             418..1926
                     /gene="istA"
                     /locus_tag="VMZ53_05230"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HUS27884.1"
                     /translation="MIDAETRARIRRLFFAEHWRIGTIATELGVHHDTVRLAVEADRF
                     ANTGTQVRPTLLDPYKGLVGKILDDHPKLRATRIYEMVKARGYVGSVVQVRRYVHGVR
                     PSPREAFLRLETMPGEQAQVDWGHFGKIQVGSATRALSCFVIVLSWSRAIYARFALDQ
                     TLESFLRGHILGFAALGGVPREILYDNLKSVVLERVGDHIRFHPRILDLAGHYHFAPK
                     PCAPYRGNEKGKVERQIQYLRHSFFAARRFSTVEDLNRQLERWIADIAHVRRAPGHAD
                     GRAVGTLLGEERPRLLTLPEHAMECDRIVAVTSGKTPYVRFDLNDYSIPHTLVRKPLT
                     LVASEVQVRIVDGQEVVARHIRSYDRGKTVEDPAHIAALARDKRHAHDLRGRDLLRSV
                     CKHADELLDALALRGEHIGGHTARLTQLLRQVGAVELDRAMAEALARGATGAGSVAHL
                     LEQRARARKQQPTLEAVLPDDPRVRGLRVTPHVLARYDDLYAKSKPDEEPES"
     gene            1923..2702
                     /gene="istB"
                     /locus_tag="VMZ53_05235"
     CDS             1923..2702
                     /gene="istB"
                     /locus_tag="VMZ53_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HUS27885.1"
                     /translation="MSDESALVEALTLIGLRHTAANLDDLVALATKRRWGPTQLLEHV
                     AEAEQADRAKRSLERRVDRSHLGRFRAMADFDWAWPKHIDRDIVDSVLQLDFLADARN
                     VVLVAPQGLGKTMIAQNIVHAALLAGHSALFITASQLLLDLGAQESTRALDRRLRYYS
                     SRALLCIDEIGYLSYDTRNADLLFQVVSRRYERRSLVLTTNLAFSEWASIFPNAACTT
                     ALIDRVVHHAEIIAIEGDSYRKREAQAAINKPKPSRRSKAT"
     gene            complement(2737..3126)
                     /locus_tag="VMZ53_05240"
     CDS             complement(2737..3126)
                     /locus_tag="VMZ53_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27886.1"
                     /translation="MMLVEVSEIDGAPPHIPAPVDLMTQLEIEVLVSQRAADHHALAV
                     PLDEALLINTCDLSKRLAEGVLLDELARVRALRWRTNRGRPSLAECFMRSYMVVVAAP
                     CVEDSLLDDVGGGGRSGFFLERSVKCW"
BASE COUNT          660 a         1300 c         1180 g          644 t
ORIGIN      
        1 ccggtctcga cgcatgacgc gccgaccgtg cgccgcgagg aggacatcga tgcgttcact
       61 ctgggttgga atcgattcga ggagtcacga gacgaggatt ctggcgatgg ccggtccgac
      121 ggagacggtc ttgaaagcgc ggctgttgcc gtcggtggcg cacccacgcg cggtgccgac
      181 gctgctcgag gcactggcgc tgtggcaggg ctcgacggtc cgtgctgcgc tcgttgtgga
      241 cgacgtggac ggttcgtccg gcacgagact cgggctcgac tcgtgggtag gactcgccgg
      301 tccgccgctg tacgaggtcg agttcgtccc ccgtcgcaag ccccaccatc gtgacgcgct
      361 cgacgggatg ggtgcgttcc acgaccttcg tcagctgacg atgttcgagg tcgcgcgatg
      421 attgacgccg agacgcgcgc tcgcatccgt cgtctgttct tcgccgagca ctggcgcatt
      481 ggcaccatcg ccaccgagct cggcgtgcac cacgacaccg tccggctcgc cgtcgaggcg
      541 gaccgattcg caaacaccgg gacgcaggtg cgcccgacct tgctcgaccc gtacaagggc
      601 ctcgtcggca agatcctcga tgaccacccc aagcttcgcg cgacgcgcat ctacgagatg
      661 gtcaaggcgc gcggctacgt cggctcggtc gtgcaggtgc gccgctacgt gcacggcgtg
      721 cggccgtcgc cgcgcgaggc gttcctgcgg ctcgagacga tgcccggcga gcaagcgcag
      781 gtcgactggg ggcacttcgg aaagatccag gtcggttcag cgacgcgtgc gctgtcgtgc
      841 ttcgtcatcg tgctgtcgtg gtcgcgcgcc atctacgcac ggttcgcgct cgaccagacg
      901 ctcgagagct tcctgcgtgg ccacatcctc ggcttcgccg cgctcggggg cgtgccgcgg
      961 gagatcctct acgacaatct caagagcgtc gtgctggagc gcgtcggcga ccacatcagg
     1021 tttcatccgc gcatcctcga cctcgccggc cactatcact ttgcgccgaa gccctgcgca
     1081 ccatatcgcg gcaatgaaaa gggcaaggtc gagcgccaga ttcagtacct gcgccactcg
     1141 ttcttcgccg cgcgtcggtt ctccaccgtc gaggatttga atcggcagct cgagcgctgg
     1201 attgcggaca tcgcgcacgt gcggcgcgcg ccgggtcatg ccgacggccg cgcggtcggc
     1261 acgctgctcg gtgaagagcg gccgcggctc ctcacattgc ccgagcacgc gatggagtgc
     1321 gaccgcatcg tcgccgtgac ttcgggcaag acgccgtacg tgaggttcga cctcaacgac
     1381 tattcgatcc cgcacacgct cgtgcgcaag ccgctgaccc tcgtcgcctc ggaggtccag
     1441 gtgcgcatcg tcgacggaca ggaagtcgtc gcgcggcaca tccgcagcta cgaccgcggc
     1501 aagaccgtcg aggacccagc gcatatcgcg gcgctcgcgc gcgacaagcg acacgcgcac
     1561 gaccttcgcg gtcgtgacct gcttcgctcc gtttgcaagc acgcggacga gctcctcgac
     1621 gccctcgcac ttcgcggcga gcacatcggc ggtcacaccg cacggctgac ccagctgctg
     1681 cgccaggtcg gcgccgtcga gctcgaccgc gcgatggccg aggcactcgc acgcggcgcg
     1741 accggcgccg gttccgtcgc gcatctcctc gaacagcgcg ctcgtgcacg caagcagcag
     1801 ccgacgctcg aggccgtgct tcccgacgac ccgcgcgttc gcggcctgcg cgtcacgccg
     1861 catgtgctcg cacgctacga cgacctctac gccaagtcga agcccgacga ggagccggag
     1921 tcatgagcga cgaatccgca ctcgtcgaag cgctgaccct cattggcttg cgccacacgg
     1981 ccgccaatct cgacgacctc gtcgcgctcg cgaccaagcg ccgctgggga ccaacacagc
     2041 tcctcgagca tgtcgccgaa gctgagcaag ccgaccgagc gaagcgcagc ctcgagcgcc
     2101 gcgtcgaccg cagccacctc ggtcgctttc gcgcgatggc tgacttcgac tgggcctggc
     2161 ccaagcacat tgaccgcgac atcgtcgact ccgtgctcca actcgatttc ctcgccgacg
     2221 cgcgcaacgt cgtgctcgtc gccccgcagg gcttgggcaa gacgatgatc gcgcagaaca
     2281 tcgtgcacgc cgcgctactc gcgggccaca gcgcgctgtt catcaccgca tcgcagctcc
     2341 tgcttgacct cggagcgcag gaatcgacgc gcgcgctcga ccgacggctt cgttactact
     2401 cgtcccgcgc actgctctgc atcgacgaga ttgggtatct gtcctacgac acccgaaacg
     2461 ccgacctctt gttccaggtc gtcagccgtc gctacgaacg ccgcagcctc gtgctgacca
     2521 ccaacctcgc gttcagcgag tgggcatcga tcttcccgaa cgctgcctgc accacggcgc
     2581 tcatcgaccg cgtcgtccac cacgccgaga tcatcgccat cgagggcgac agctaccgca
     2641 agcgcgaggc tcaggcggcg atcaacaaac cgaagccgtc acgtcgctcg aaggcaacat
     2701 gatcaggccg gtccaaattt ccgcgcttcg aggcgactac caacacttca ccgaacgctc
     2761 aaggaagaaa ccgcttcgcc cgccgccgcc gacatcgtcg agcagcgagt cttcgacgca
     2821 tggcgcggcg actacaacca tgtacgacct catgaagcac tcggccagcg acggccgacc
     2881 acggtttgtg cgccatcgaa gcgcgcgtac ccgtgcaagc tcgtcgagaa ggacaccttc
     2941 tgcgagccgt ttggagagat cgcacgtgtt gataagcagg gcttcatcaa gtggcaccgc
     3001 caacgcgtga tgatcagcag cgcgctgcga tacgagtacc tcgatctcga gctgagtcat
     3061 gaggtcgacg ggcgctggga tgtgaggtgg ggcgccatct atctcggata cctcgacgag
     3121 catcatcgcg atcgaggctt cattcctgtt cgtcggaaac gtggcgtggg tcaccacgtt
     3181 gtctttcgtc gaggtcgacg agtagctgtt cagcttcgat acgaaccaca tgccgccgcg
     3241 gccgagctcg cggatcacca cgcagcctgc gaacctcgga tagtccgagt aaccagaacc
     3301 gacaacgaga tagtccaatc tcggacagat gaagcaggct gaatggagca tgcagcagca
     3361 cgctctgtcc gaacttcgga cagttgttcg cagctgcaga cagcgcgact gtccgaatct
     3421 cggacagctt cgcggagcta agtttgcact ccgatttcgc ggagtgagcc ctacttcttc
     3481 ctctcgcgca tcgcgttctc gagcgcggtt cggatctcga cggtgtatgg gatctgcgag
     3541 ggctcggtgt aagggatgtc cgtgggcaaa agtgtcaagg atgtctgtgc ttgatcagga
     3601 cccgaatccc aagggcagcg agcgacgttc gctctccacc accacctgtt cgagcactct
     3661 acgtccgcga gtccggccga tggcggtgat tccgccgatg tcaggtcgac gtcgacacct
     3721 cgctcgtgcg tcgcgcgagc gcgcccgtgg ttcggtctcc cgcgagctac cgaggccgcg
     3781 agat
//
LOCUS       DATLBZ010000100        22178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5308573, whole genome shotgun sequence.
ACCESSION   DATLBZ010000100 DATLBZ010000000
VERSION     DATLBZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22178
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5308573"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VMZ53_05245"
     CDS             complement(<1..539)
                     /locus_tag="VMZ53_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27887.1"
                     /translation="MAAIESGWTRFYGMPVPQEVAAAATEHMIDTSVADLVEAHLGIN
                     YSYFGAIDKTLSGFVIIDDEGDNYTLIDLRDTGQTWWQDHETREIELRGAKTAKPARK
                     VVATPDLLARYQWLVWQLARPMEQNGKPIQTIDYLVRNGIGRFRHLFPRKEAYEKTFA
                     AELPALSRDPHLAIYWLLHT"
     gene            582..3557
                     /locus_tag="VMZ53_05250"
     CDS             582..3557
                     /locus_tag="VMZ53_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27888.1"
                     /translation="MRVIDQARLWFREGTSDKVYEVDLVEVATGQYVVNFRFGRRGGA
                     LKDGTKTSLPVSQEKARGIFTKLVDEKLAGGYAHAGQAAPPRPPPATTPLTPTVSVVH
                     TSEAQTLIASLRQGARSSTPLGPVVWRVTDLDLYDADPVLRELLGSTFVPKDFVATEW
                     QNLLLSALARCGRLDSLPVLARFDAPNQPHHLRYLAQLAMAKIDPPRADTLARPVLTP
                     ALLAAYVASDPAALARAAEELLAVDPIKARDCAVALYLLNPAVARRAVLAIARVARLS
                     NAEATVVRTLFRVAEVRRDGELYALLARRIDAYTAPRRPFSPKTREYIRRRVARVLRR
                     LGRAQSPDYVKMAAAILLGYDDEDAVPAKRGSFGHQYDTFAPFHAFNQILYANSPRYE
                     KSHHKKSAWRVTRAWRANAPAPVQREEAFPELWDRAPDVLWAVILKSGATPVIEFATR
                     ALRANRTYCDALRDEQVASVLAMGHPLAQEFAFDIARTRPLNTTLARAALSSDVAAAH
                     RWVTSWIEQHADEAAADADLVAMLVTGKTQQIREAALDLVRKKALPDAVARSAAARAL
                     AILLGLANTPANAERASGAVTAMMRVLEPALRELGPEVLRDMVKHPLAGLGELAGEII
                     LRHAQRDSLPGDLLEAMLASPHATVRTLGGRLLAETPPEIAKDDLEALVLFATSGNAE
                     LREATRTLLGKIAVQYPDVGRALADQLVEALLRTQPPGAPAHIISLLRAELAGVLPRK
                     SAQTILRLISALSPHARDAGGLLLPQLTADDIGLDDISRLASHENKAVREGAWALARG
                     STDRYRIAPVALAKLVDSQWDDTRAFAIDFIDQHIGQLSAEAIITICDSVRPDVQDLG
                     KQLLHRQFREADAGKYLVRLAEHPSVNLQLLVSGLLDRHAGTVDKLRTLVPYFATVLS
                     QVNRGRVAKERVMALLRREAARSAEHAAVLAPLLERQSATAAVTQKAPIIATMVDVHH
                     QFPEVPLPIKVTAPKGAES"
     gene            3554..6082
                     /locus_tag="VMZ53_05255"
     CDS             3554..6082
                     /locus_tag="VMZ53_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27889.1"
                     /translation="MIFEHRYAGSSAVQQTEGATAMAFAPDTLRQPTYFRGSITKQVE
                     FREAISALHDVVISDLRFIPKDRTDYLAWRERQSYVDLAEISTQQRKLSDDIKVATDE
                     LTRLEQAASFRRSGFYKARQKYFDYIYKRDIDLWYKLDPVITVHPDQVFFECFSRDES
                     SYGRLAASYDVFSELGERACGTTNVDYSDKLYAEFQKIRSYKTTTLDVDPSGFNVKTS
                     LEQAHKEVKIDLPDSWVRGFLQVSSAATLNANVVELHPMDVHNLCLILRRNKELFGPR
                     SLRFKLTPGAPVEIVVEPWNHVVKCPRSRQTDGTGGEIRVWGRRRLHILERLLPRAKR
                     VRVFLLGTGLPTFWVVDLGDLSFTLGLSGWTQNNWSESGNFDLMAAREDVDDITKQRV
                     FAELGKSWLATPEQLAASTGLATPIVASALAGWVQAGRAIYDLDKGVYRKRELTREPL
                     PMDKLRFANPREDAAAQLLHRGKIAIDRTDTAEGLVLEGRVLDTLHGKQKLYAPKITF
                     DGDRRLVAGECSCDYFIRNRLHRGPCEHMLALRAAHRRGISDKIDIQPTSAAPLRPGQ
                     VPPQASQNFQQAYRQGIMRASQLRQSGRTQDALAELERTARMAPPDSDEMGRLQEVIA
                     EARFDAGDMTPARQAAERALSRFPTSVLALRIKRDALVELDDVTTAIPVARALAHVDN
                     KVERWDELCVLCDSIADHRAMYQFAEEGLRLHANNAKLTAHRDRARANLPANTPLPTA
                     AQADAAKPSWWRRAVGAITGRPPTTPPPTLDSRLDAILGELAKGSVIRDRTTVLQILR
                     IAHGSADRDEARVFALAAALKDCPAVTNLPSDQDLLALLRRALQ"
     gene            6336..7757
                     /locus_tag="VMZ53_05260"
     CDS             6336..7757
                     /locus_tag="VMZ53_05260"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HUS27890.1"
                     /translation="MSDLSPPKNRQELYARIARGGKDEVILDEMVRLGFWPNTLPIPS
                     DPPDEVRRIAELRSRLTQLRNQAKGLRNLAQLEIDLKKKRMAESKRRREQNKQKLLGE
                     RAAARAETQQKKTRDLTYLGPQVSAGLGPKPDRRTSDTAKLTAAKIPVAATAAELAAL
                     MKLPLGQLRFFAFSREVSTTTHYHRFTIPKKTGGERVISAPHKRLKAAQNWILKHVLE
                     PLALADAAHGFVPGRSTVSNALPHVGAAIVVNVDLKDFFPTVTYKRTKGLFKKLGYSE
                     EVATVLGLVCSEPDITQTELDGTTFYVARGPRRLPQGAPTSPAITNALCRRLDRRIMG
                     WAAKHGFTYTRYADDLTVSTKDPKARVQSMLAFLRHVAQEEGFVVHPDKVRVVRRARR
                     QEVTGVVVNDRPGIPRDDLRRFRALLHHIDKDGPAGKTWGHSTAPVLDVALGFASYVA
                     MVDAAKGEQLRAKVLALRAKYPR"
     gene            complement(7754..8380)
                     /locus_tag="VMZ53_05265"
     CDS             complement(7754..8380)
                     /locus_tag="VMZ53_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27891.1"
                     /translation="MSGCSFIGARVPKNPTPDHHCPTGVVIADGVGTAFFLIEPTACV
                     IDGALGGFEGENDGLACVMAGVPLAIVGSVYLLSTIYGSRAHGRCERRKGAYVADVER
                     QEKRGGDERVRAAVVGDAAMVCAVSEPDVGPCFADDATCAAEVKRAGGACEAKQETWC
                     FDMQSIVGGPIETTCAVSRVDCDARRIGYTRDASLVVTTCGSYTARAP"
     gene            complement(8419..8688)
                     /locus_tag="VMZ53_05270"
     CDS             complement(8419..8688)
                     /locus_tag="VMZ53_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27892.1"
                     /translation="MTPFVKELLLAELTRRGYHEGKSPNGTIVLDVAELIELDIAEFL
                     DLMVTRREKISRSIEALGKDKARAGFEDADHAVEAIKTVIKKLTD"
     gene            complement(8685..10502)
                     /locus_tag="VMZ53_05275"
     CDS             complement(8685..10502)
                     /locus_tag="VMZ53_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HUS27893.1"
                     /translation="MSSTALHYRPNLRDIFFNLFEVLEIQKTVLGKAPFESMDEDSAR
                     AALEGFLEVLQTTWSPAFVPGDREGATFDGKGNVKLPAAYHKALDAYFNGGWNNIELP
                     EKLGGFGAPPSVRWSAFELMSGANPSVTFYVLGNVMSTIIDNFGTPSQKARFIKPMID
                     QHWGATMVLTEPAAGSDVGAGTTKARHIEGDVYELEGVKRFITNGDFDHSKNIIHMVL
                     ARPEGAGPGTKGLSMFIVPKFWVNEDGSLGERNGAVVTNVEHKMGIRGSATCELTLGA
                     DAKCRGLLLGEVHDGIAQMFHVIEYARMGVGTKSMATLSTAYLNALDYTRVRKQGADL
                     TKAADKSAPRVEIIKHPDVRRMLMLQKTFAEGLRAIVLWTANVQDQVALRGGHGNQEA
                     RPLDALNDFMLPMIKGYGSEKVYELLAVALQCFGGSGFCQDYPIEQYIRDQKIDSLYE
                     GTTHIQALDLFFRKIGRDRGATLQALVGQITATLDGLPAELAPEKAALQQAVADCQGI
                     MMTMMGKLGQSVYHVGLQGNRILFALAELVIGWRLAVNAKVALGKLSTATGDDVAFYK
                     GKVAVARFYAKNVLPGLSLAKKLIEQSDLDLMELSDDCW"
     gene            complement(10520..11323)
                     /locus_tag="VMZ53_05280"
     CDS             complement(10520..11323)
                     /locus_tag="VMZ53_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27894.1"
                     /translation="MRSTLRFVCAGLLAACGGGGGFPDAKELDAAPTGTFSATWSVID
                     QNNQPLSCERIGGNTMTVLAHNKAFDGGSTQIFTCGTGMGTSQAVIAGNYDFQFELSS
                     AMFGLLATATAQTNVAVPPGGPTPLAPLTFQVQATGAMELKLATGKPGGNCGAATNGA
                     GITGVTITMNHTSDTSCAPITLTISNGATQTGGTYTIDCTTPVTFGCIESDQTITASG
                     VTSDGYTMHITGFVGSTACWLNNDSVQVPPLDKTLQRTLNLAQQTTTGC"
     gene            complement(11340..11858)
                     /locus_tag="VMZ53_05285"
     CDS             complement(11340..11858)
                     /locus_tag="VMZ53_05285"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS27895.1"
                     /translation="MQTDLAISVRRATAADASALAALVNRAYSIEEFFVEGDRTNATE
                     IAGLIEKGTFIVLEYAGGLAAAVWVETSRERGYFGMLSVLPEMQKHGLGKRLVRIAEA
                     MCEAAGCDEMTLQIINLREELARWYRSLGYVEIGTKPYEHRAVKQPCHFVQMRKSLRS
                     VTGLGAVKAAAA"
     gene            12317..13336
                     /locus_tag="VMZ53_05290"
     CDS             12317..13336
                     /locus_tag="VMZ53_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27896.1"
                     /translation="MIQTHAPIAAPQRVGDATAKGSVTGVQILASVDGGAASDRPTYA
                     RKDQKVTLYALVRVGAAFYTDAPSPHLAGKAISAQPLAKAPSIDLAWQRIEPTTANMS
                     NTQSGSFKFEAIEYTTSAIAAANQPSITADVRPTLTTDHGDGIGTMRYQLVATQGDRV
                     LASPGIDAKRGRGSGGLGDAVTRVTIRRDDSFLGYLTEMYGQPYIWASAGLSDGSHES
                     EHLEGSDCADLMVYGARRMGKKVAYTWTGGLPNVTKLVAGSGTRGDDGIYRDKAGKPL
                     PFTAVGDLVLFPRHVGALAEDRGTIGVLDDQDIMLHTLFDSPKEQAIADSGYADTAVE
                     LRRWK"
     gene            complement(13337..14500)
                     /locus_tag="VMZ53_05295"
     CDS             complement(13337..14500)
                     /locus_tag="VMZ53_05295"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HUS27897.1"
                     /translation="MPQIAGFRGALWDASKVDLKKVSAEPMTHVKDRLAKGELVRDPT
                     RAMYRYDQSFDAGARTLTRTTLLCAIRLSPWSDKLICPHEETNPTARDAAIAAITEAG
                     AHCDPVFAGYRDAAREVDRLLRNIDRDRPVLEATTPDKTVHRLWRISSAELIGKLRPL
                     FAPKKLHVLDGHARYEGMLAYAAKLGADKQPQYSSANYGLGCLVNLEDPALAVAPRHR
                     LVRNDGLKREAVLEAAKRYFVVDKLEGAARDLGKQQASLADHIAHQPAFVVTFASHAD
                     AYKLTLKPDVSLAGEGIDIHRAIQRYDPVVIEHLFMRRVLQTVSTTSTDAEGVLDAVK
                     GGAAVGVIARPVPLSEMLHADEVGALLPFGSTAFHPPLANLCAFIVDPDEDLV"
     gene            complement(14515..15771)
                     /gene="purD"
                     /locus_tag="VMZ53_05300"
     CDS             complement(14515..15771)
                     /gene="purD"
                     /locus_tag="VMZ53_05300"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HUS27898.1"
                     /translation="MKILVLGTGGREHALVWRLAQGGNTVIAAPGNPGIEQIAACFPV
                     PLTDHAGMVRLAKEQAVDLVVVGPEAPLVAGIADELRAHGILTFGPGASGARLEGSKA
                     FSKQFFARHSIPTARFFVVNSVTDADRAIQELGGQVVVKADGLAAGKGVVVAADATEA
                     RAAARDMLEAKRFGDAGGTVIIEERIVGREVSVLAITDGKTLEVLPAVEDHKTIFDGD
                     TGPNTGGMGTVSPAWVSTDVCERITREILKPTVKGLASDGIDYRGVLFAGVMVDPMGA
                     PYLLEYNCRFGDPETQPIMARMVGDFASVLAGAARGELPHGVLAWDARVAVCVVVAAA
                     GYPGQVRTGDPIGGLDAVPDDVTVFHAGTARRPEDGRIVTSGGRVLGITARGVSVQQA
                     RERAYAVVDAIELPGKQARRDIGARS"
     gene            15858..18299
                     /locus_tag="VMZ53_05305"
     CDS             15858..18299
                     /locus_tag="VMZ53_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS27899.1"
                     /translation="MATLGSAQALAQPAGDWGVKRDPFDLTVVARYRAILAKNPYDVG
                     ALDQLHQMYSRYRTAYELEKSYSSSTDWATAVVYARIAKDDIDRWKKVVELKADDARG
                     LNALADAERGQGATNEAFGHYQKAAQLASDNATKKNALEWTARLAEDFQNITRLEWAY
                     AQLIALEPKEPKHWTNRALSMQKLNRYTDAIESFAKAEALTTTDPQGKLDLILLRARA
                     LVQIGDTHAALEQYNRVLQSAPRGYYLADEAITALIDLHLAAGTLPEFRLALEKRWPP
                     GMRAYPEWKTLANVYEQLGDTEGAIFALKKAVTAAPSERDTHTKLVELYDGNGETDLA
                     LAQLEAATKALPADQDFHVQLANRYHDAKETKKAFATLQRLSQTIRTDARVHEDLADL
                     YTKWKRPGLALHELEEIARIEPEPENIAKLGEAYWQGGSEDKALATWKKLGSDPEGLA
                     RLAQVYVDHEFWPDAIVVYTQALKLAPKNAELWRARADVHETLEHWEDAFADATKAAT
                     LVKGRTLDEGHQVRYQLVRLLSRWAEHETDGENTHEAAAVRAWTRDFVAEPPDLEAGY
                     MLAEYEGRSPSADSLAALERLHKLVPTDLGVEQTLARTYKILHRYNEAIAQLESLTLA
                     DPNKALDYQDQIADLKKRIDLDGREGWRDDGIADPDAWVRAHRKPDPIEPFRLGLHMG
                     VGNAMRGPAPHSVLVGMYTSIRIGRRVAFVGRFDWSERGGDMTSVKSAALATGVALPV
                     LNLSRMVVTLGAAHRTELRIGDHMGWDRLGLAGDFTLELIPKKMPGSLAIRFEQGLSD
                     HGQSTALLVELGVGN"
     gene            18317..21157
                     /locus_tag="VMZ53_05310"
     CDS             18317..21157
                     /locus_tag="VMZ53_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HUS27900.1"
                     /translation="MKKLVAALVVVAAVVIAFVMWPRERAHPKSTAPANAAGSATTAV
                     TFRGTDGVERTLPAWMLSPGAPAKRIAGRVIFRGEPARDAIVELHSILSSTNLSAAVV
                     VRTDANGHFDFGVRPAAAYDVTATRPDTTAAIQHVELANPTLTPASDRLELVLGECTN
                     SIEGTVVDASNDPLPKARIRQNGFVGPETDAKGHYKLCVAFGDAELHYSADGYGSVVL
                     TIDARGQMRRDVVLVPEATLDVTVVREDDAKPVEGALVVAGPMQWGPDRAGPSRGLTG
                     ADGRVRLGGLVPGRYHIFALGDDAQSSAPAEALAQVGVATDVVLKVAATARVTGHVVS
                     GTKPVAGAEVTAVRKAPMGRSPVVFSQLDGSFTLDRVPAGDISFVAAPYEVESPASIA
                     AAAGKSVDVTIKVHALGAIRGRVLRLGKPVPDVRVCCVQTIAGLRPDAITRADGTFEY
                     RGVSAGEYDLQGGSDELGAFNMPVHVKLAAGEEKSVDLDLDMAGTITGTVVDKSGAPV
                     PSVFVRWVHQTSGDLGRCMTNAKGEYRCGTMTGGGTYRAGVFPSSELQGVPYPTATGA
                     PYPSVDVADAKTLVEGVTIAIDHAQQTISGRVIDDTGAPVVDVIVKAMPTGEAAPQFN
                     SWLRLPMTSTDADGSFTLNELAPGQYALEAHAADGGEGMVTGVAAGAKSVAIKIERAG
                     SITGTLVGFTKPPVVYATPQGPFKLFTGTVDGQSFRVTGLRPGRYTVNAQTAIEGDIQ
                     QVDVKAGQPAKVTLTSHGKGTIEGTVFEFRTKKPLGGATCRVVMQMNGEQSQTSWDPA
                     LAPKSDGGGRIVIDPAPAGPVSVICQPMGFKWSLATTDVTLTAGARATPQLMAVELTQ
                     DNPTSTGAQLDWRVTPPRIARVMPNTPAAAAGLLAGDLITAVDGVSVAGLCGSAVSVL
                     ISNTAANSDVKLTVQRGTATKTVSVKALHMPD"
     gene            21308..21688
                     /locus_tag="VMZ53_05315"
     CDS             21308..21688
                     /locus_tag="VMZ53_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27901.1"
                     /translation="MKILNVVAPCVCLLSFAGCAADSTDMGSVTISTYSLHVAREGDG
                     AVAGAMPRFVIKPTSGGNPTSIKAWVGLEAIDATGKFLAAYDSADGDFDADVTVPTPL
                     PTGAQLWLDVETTGTTTTGSIALK"
     gene            21703..21906
                     /locus_tag="VMZ53_05320"
     CDS             21703..21906
                     /locus_tag="VMZ53_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27902.1"
                     /translation="MITIKITNVEDIVEKNKGWFVANVVGAFVDLESRVEDVIITKLR
                     ESLAREGVEAVIERAPSQPPRAP"
     gene            21940..>22178
                     /locus_tag="VMZ53_05325"
     CDS             21940..>22178
                     /locus_tag="VMZ53_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27903.1"
                     /translation="MRAVLFAVLFTFGCSSNKENANPSPSASAPTSDRRADDRATEAE
                     ESRHVAKVEAATAKEAADVKLTAAHDETAARLQKE"
BASE COUNT         3903 a         7576 c         7073 g         3626 t
ORIGIN      
        1 gtgtgcagca gccaatagat cgcgagatgc ggatcgcgcg acagcgccgg cagctccgcg
       61 gcgaacgtct tctcgtacgc ttccttgcgt ggaaacaagt ggcggaatcg gccgatgccg
      121 ttgcgcacga ggtagtcgat cgtctggatc ggcttgccgt tctgctccat cgggcgggcg
      181 agctgccaga cgagccactg atagcgggcg agcaagtccg gtgtcgcgac gaccttgcgg
      241 gcgggcttcg cggtcttcgc tccgcggagc tcgatctcgc gcgtctcgtg gtcctgccac
      301 cacgtctggc cggtgtcgcg cagatcgatc agcgtgtagt tgtcaccttc gtcgtcgatg
      361 atgacgaacc ccgatagcgt cttgtcgatc gcgccgaagt acgagtagtt gattccgagg
      421 tgcgcctcga cgagatcggc gacggacgtg tcgatcatgt gctccgtcgc cgccgcagcg
      481 acctcctgcg gaaccggcat gccgtagaag cgcgtccacc cgctctcgat cgctgccatc
      541 gtggcgagtt aatcacacga ccgtttgata ggctgcgcga cgtgcgggtg atcgatcagg
      601 cacgtctgtg gtttcgcgaa gggacgtcag acaaggtcta cgaggtcgat ctggtcgagg
      661 tcgcgacggg ccagtatgtc gtcaacttcc gcttcggccg ccgcggtggc gcgctcaagg
      721 acggcacgaa gacgtcgctg ccggtctcgc aggagaaagc gcgcgggatc ttcaccaagc
      781 tcgtcgacga gaagctcgcc ggcggctacg cacacgcagg tcaggccgcg cctcctcgcc
      841 caccgcccgc gacgacgccg ctgacgccga cggtctcagt cgtccacacg agcgaagcgc
      901 agacgctgat cgcgtcactc cgtcagggcg cacggtcgag cacgccgctc gggccggtcg
      961 tctggcgcgt gacagacctc gatctgtacg acgccgaccc cgtgctgcgc gagcttctcg
     1021 gctcgacgtt cgtgcccaaa gatttcgtgg cgacggagtg gcagaacctg ctcctcagcg
     1081 cgctcgcccg ctgcggaagg ctcgactccc ttcctgtgct cgcgcgcttc gatgcaccga
     1141 accagccgca tcacctgcgc tacctggcgc agctcgcaat ggcgaagata gacccgccgc
     1201 gcgccgacac cctcgcgcga ccggtgctca cacccgcgct cctcgccgcg tatgtcgcga
     1261 gtgatccggc agcgctcgca cgagccgccg aggagctgct cgcggtcgat ccgatcaagg
     1321 cccgcgattg tgcggtcgcg ctgtatctac tgaatcctgc ggtcgcgcgg cgcgccgtac
     1381 tcgcgatcgc gagggtcgcg cggttgtcga atgccgaggc cactgtcgtg cgcacgctat
     1441 tccgggtcgc cgaggtgcgc cgcgatggcg agctctatgc cttgctcgct cgccgcatcg
     1501 acgcatacac cgcgccgcgt cggccgttct cgccgaagac gcgcgagtac atccgtcgcc
     1561 gcgtcgcgcg cgtcttgcgc cgcctcggtc gagcgcagtc gccggactac gtgaagatgg
     1621 ccgctgcgat cctgctcggc tacgacgacg aggatgccgt gccggcgaag cgtggctcgt
     1681 tcggccatca gtacgacacg ttcgcgccgt tccacgcgtt caatcagatc ctctacgcga
     1741 acagcccccg ctacgagaaa tcgcatcaca agaagtccgc gtggcgtgtg acccgcgcgt
     1801 ggcgtgcgaa tgcgccggca ccggtccagc gcgaggaggc attccccgag ctgtgggacc
     1861 gcgcacccga cgtgctgtgg gcagtgatcc tcaagagcgg cgcaacgccg gtgatcgaat
     1921 tcgcgacgcg cgctcttcgc gccaaccgca cgtactgcga tgcgctccgc gacgagcaag
     1981 tcgcatcggt gctcgcgatg ggacacccgc tcgcgcagga attcgcgttc gacatcgcgc
     2041 gcacgcggcc gctcaacacc acgctcgccc gtgccgcgct ctcgagcgat gtcgccgcgg
     2101 cgcaccgctg ggtcacgagc tggatcgagc agcacgccga cgaggcagcc gccgacgccg
     2161 acctcgtcgc gatgctcgtc accggcaaga cgcaacagat tcgcgaggcc gcgctcgacc
     2221 tcgtccgcaa gaaggcgctg cccgacgccg tcgcgcgaag tgccgccgcg cgcgcgcttg
     2281 cgatcctcct cggtctcgcg aatacaccgg caaacgccga gcgcgcgtcc ggcgccgtca
     2341 ccgcgatgat gcgcgtcctc gagccagcgc tgcgtgagct tggccccgag gtgctgcgcg
     2401 acatggtgaa gcatccgctc gcgggtctcg gcgagctcgc cggagagatc atcctgcgcc
     2461 atgcacagcg cgactcgctg cccggcgacc tcctcgaagc catgctcgcg tcgccgcacg
     2521 cgacggtccg cacgctcggc gggcgcctcc tcgccgagac gccaccggag atcgcgaagg
     2581 acgacctcga agctctcgtg ctgttcgcga cgagcggcaa cgcggagctg cgcgaagcaa
     2641 cgcgcacgtt gctcggcaag atcgctgtgc agtatcccga tgtcggtcgc gcgctcgccg
     2701 accagctcgt cgaggcgctg ctgcgcacgc aacctccggg cgcgccggcg cacatcatct
     2761 cgctgctccg cgcggagctc gccggtgtgc tgccgcgcaa gtccgcgcag acgatcctgc
     2821 ggctgatttc ggcactctcg ccgcatgcac gcgacgcggg cggcctgctc ctgccgcagc
     2881 tcaccgccga cgacatcggc ctcgacgaca tcagccgcct cgcgagccac gagaacaagg
     2941 cggttcgcga aggcgcgtgg gcgctcgcgc ggggctccac cgaccgctat cgaatcgcgc
     3001 cggtcgcgct cgcgaagctc gtcgactcgc agtgggacga cacacgcgca ttcgcgatcg
     3061 acttcatcga tcagcacatc ggccagctgt ccgccgaggc gatcatcacg atctgcgact
     3121 ccgttcgccc cgacgtgcag gaccttggca agcagctcct gcatcgccag ttccgcgaag
     3181 ccgacgcggg caagtacctc gttcgactcg ccgagcatcc gtccgtgaat ctgcagctgt
     3241 tggtgtcggg cctgctcgat cgtcacgccg gtaccgtcga caagctgcgc acgctcgtgc
     3301 cgtacttcgc gacggtgctc tcgcaggtca atcgcggacg cgtcgcgaaa gagcgcgtga
     3361 tggcactgct ccgtcgagaa gcggcgcgca gcgccgagca tgccgcggtc ctagctccat
     3421 tgctcgagcg ccaatcggcc acggccgcgg tcacgcagaa ggcgccgatc atcgcgacga
     3481 tggtcgacgt ccatcaccag tttcccgagg tgccgttgcc gatcaaagtc acggcgccca
     3541 aaggagcgga gtcgtgatct tcgagcatcg ctacgcgggc tcgtccgccg tgcagcaaac
     3601 cgaaggcgcc actgcgatgg cgttcgcgcc cgacacgctg cgccaaccga cgtacttccg
     3661 cggcagcatc acgaagcagg tcgagttccg cgaagcgatc agcgcgctgc acgacgtggt
     3721 catcagcgac ctccgcttca tcccgaagga tcgcaccgac tacctggcgt ggcgcgagcg
     3781 tcagagctac gtcgacctcg ccgaaatcag cacgcaacaa cgcaagctct ccgacgacat
     3841 caaggtcgcc accgacgagc tcacgcgcct cgagcaggct gcgtcgttcc gccgctcggg
     3901 cttctacaaa gcgcggcaga agtacttcga ctacatctac aagcgagaca tcgacctttg
     3961 gtacaagctc gacccggtca tcacggtcca cccggaccag gtgttcttcg agtgcttttc
     4021 gcgcgacgag tcgtcgtacg gccggcttgc cgcgagctac gacgtgttca gcgagctcgg
     4081 cgagcgcgcg tgcggcacga cgaacgtcga ctactcggac aagctgtacg ccgagtttca
     4141 aaagatccgc agctacaaga cgacgacgct cgatgtcgat ccatccggct tcaacgtgaa
     4201 gacgtcgctc gagcaagcgc acaaagaggt caagatcgac ctgccggact cctgggtgcg
     4261 cggcttcctc caagttagct ccgccgcgac gctcaacgcg aacgtcgtcg agctgcaccc
     4321 gatggacgtc cacaatctgt gcctcatcct gcgccgcaac aaggagctgt tcggcccccg
     4381 ttcgctgcgc ttcaagctga cacctggtgc gcccgtcgag atcgtcgtcg agccgtggaa
     4441 ccacgtcgtg aagtgcccac gctcgcgcca gaccgatggc accggcggcg agatacgcgt
     4501 gtggggccgg cgtcgcctgc acatcctcga gcgcctgttg ccgcgtgcga agcgcgtgcg
     4561 cgtcttcctg ctcggtacgg ggcttccgac gttctgggtc gtcgacctcg gcgacctgtc
     4621 gttcactctc ggcctctctg gttggaccca gaacaactgg tccgagtccg gcaacttcga
     4681 tctgatggcg gcacgcgagg acgtcgacga catcacgaag cagcgcgtgt ttgccgagct
     4741 cggcaagtcg tggctcgcga cgcccgagca gctcgcggcc tcgacgggac tcgcaacgcc
     4801 gatcgtcgcg tcggcgctcg ccggttgggt ccaagctggc cgcgcgatct acgacctcga
     4861 caaaggcgtg tatcgcaagc gcgagctgac gcgcgagcca ctgccgatgg acaagctgcg
     4921 gttcgcgaac ccgcgggagg atgcggcggc gcagctgctc caccgcggca agatcgcgat
     4981 cgatcgcacc gacacggccg agggcctcgt cctcgaagga cgcgtgctcg atacgctgca
     5041 cggcaagcag aagctgtacg cgccgaagat cacgttcgac ggcgatcgcc gcctcgtcgc
     5101 gggcgagtgc agctgcgact acttcattcg caatcgcctg caccgtggcc cgtgcgagca
     5161 catgctcgcg ctgcgtgccg ctcaccgccg cggtatcagc gacaagatcg atatccaacc
     5221 gacctcagcc gcaccgctcc gccccggcca agtcccaccg caggcgtcgc agaatttcca
     5281 gcaggcatat cgccagggaa tcatgcgcgc gtcacagctc cgccaatcgg gtcgaacgca
     5341 agatgcactc gccgagctcg agcgcaccgc acggatggcg ccgcccgaca gcgacgagat
     5401 gggtcgcctc caagaagtga tcgcggaggc acggttcgac gcgggcgaca tgacgccagc
     5461 gcgccaggcc gccgaacgtg cgctctcgcg tttcccgact agcgtgctcg cgctgcgcat
     5521 caaacgcgat gcactcgtcg agctcgacga cgtcacgacc gcgatcccgg tcgcgcgcgc
     5581 gctcgctcac gtcgacaaca aggtcgagcg ctgggatgag ctctgcgtgc tgtgcgattc
     5641 gatcgccgat caccgtgcga tgtaccagtt cgctgaagaa ggcctgcgcc tgcacgcgaa
     5701 caacgccaag ctcaccgcac accgcgatcg cgcacgcgcg aaccttcccg ccaacacgcc
     5761 gctgccgacc gccgcgcaag ccgatgctgc aaagccatcg tggtggcgtc gcgccgtcgg
     5821 tgcgatcacg ggccgcccgc cgacgacgcc accgcccacg ctcgactcgc gcctcgacgc
     5881 gatcctcggc gagctcgcaa agggctccgt gatccgcgac cgcacgaccg tgctccagat
     5941 cctgcgcatc gcgcacggct cggcggaccg cgatgaagct cgcgtgttcg cgctcgccgc
     6001 ggcgctcaaa gactgtcccg cggtgacgaa ccttccgtcc gatcaagacc tgctcgcgct
     6061 cttgcggcgc gcgctgcagt gaccgatatt tcgcgacgcg tggtgggcgg tcctagacct
     6121 tgcaatcggg cggcggatcg ccgtccttgc ggtaaacctg cccatacatc actggctcac
     6181 tcaatactgt gtgtccgcag gcaccatcag gagattccga gggggtttcg tgaaagcgaa
     6241 tgcacccgat ggtacccgcg gccactcgag ccgagcgatc cagtcccagg cgtagacgaa
     6301 ggtaaccgtc caccacgcac ttttttaccg acgccatgag cgacctctcg ccgccgaaga
     6361 atcgccagga gctctacgcg cgcatcgcgc gtggcggcaa agacgaggtc attctcgacg
     6421 agatggtgcg gctgggcttc tggccgaaca ccctgccgat tccgagcgat ccgcccgacg
     6481 aagttcgccg catcgccgag ctgcgcagcc gcctcacgca gcttcgcaat caagcgaagg
     6541 gcctgcgcaa cctcgcgcag ctcgagatcg acctcaagaa gaagcggatg gcggaatcca
     6601 agcgccgccg cgagcagaac aagcaaaagc tcctcggtga acgcgctgcc gcgcgcgccg
     6661 aaacgcagca gaagaagacg cgcgacctca cgtacctcgg cccgcaggtc tctgcaggcc
     6721 ttggccccaa gccggaccgc cgcacttccg ataccgcgaa gctgaccgcc gccaagatcc
     6781 cggtcgccgc aaccgccgcc gagctcgccg cgctcatgaa gctgccgctc ggccagctgc
     6841 ggttcttcgc attctcgcgc gaagtctcga cgacgacgca ctaccaccgc ttcacgatcc
     6901 cgaagaagac cggcggcgag cgcgtgatct cggcgccgca caagcgcctc aaggccgcac
     6961 agaactggat cctgaagcac gtcctcgagc cgctcgcgct cgccgatgcc gcacacggct
     7021 tcgtcccggg ccgctcgacc gtgagcaacg ccctgccaca cgtcggcgcc gcgatcgtcg
     7081 tcaacgtcga cctcaaagac ttcttcccga cggtcacgta caagcgcacc aaaggcctct
     7141 tcaagaagct cgggtacagc gaagaagtcg cgacggtcct cggcctcgtc tgcagcgagc
     7201 ccgacatcac gcagaccgag ctcgacggca cgacgttcta cgtcgcgcgt ggtccacgcc
     7261 gccttccgca aggcgccccc acctcgcccg ctatcacgaa cgcgctctgc cgccggctcg
     7321 accgccgcat catgggctgg gccgcgaagc atggcttcac gtacacgcgc tacgccgacg
     7381 acctcaccgt ctcgacgaag gatccgaagg cgcgcgtcca atcaatgctc gcattcctgc
     7441 gtcacgtcgc gcaggaagaa ggcttcgtcg tccatcccga caaagtgcgc gtcgtgcgcc
     7501 gtgcgcgccg ccaagaagtc acgggcgtcg tcgtcaacga tcgccccgga atcccgcgcg
     7561 acgatctgcg ccgcttccgc gcgctgctcc accacatcga caaggacggc cccgcgggca
     7621 agacgtgggg ccactcgaca gcgccggtgc tcgacgtcgc cctcggcttc gcgagctacg
     7681 tcgcgatggt cgacgccgcg aagggcgagc agctccgcgc gaaggtgctc gcgctgcgcg
     7741 caaagtatcc gcgctaaggc gcgcgagctg tgtacgaccc gcacgtcgtc acgacgagcg
     7801 atgcatcgcg cgtgtacccg atacgccgcg catcacaatc gacgcgcgac acggcgcatg
     7861 tcgtttcgat cggtccaccg acgatcgact gcatgtcgaa gcaccacgtc tcctgcttcg
     7921 cctcacaggc gccaccagcg cgtttgacct cggctgcaca cgtcgcgtca tcggcaaagc
     7981 acggcccgac gtccggctcg ctgacagcac acaccatcgc tgcatcgcca acgaccgctg
     8041 ctcgcacgcg ctcgtcgccc ccgcgcttct cctggcgctc gacgtccgcg acgtacgcgc
     8101 ccttgcgccg ttcgcatcgg ccgtgcgcac gggagccata gatcgtcgac aggagatata
     8161 ccgagcccac gatcgccagt ggcacaccgg ccataacgca cgcgagtccg tcgttctccc
     8221 cctcgaatcc ccccaacgcc ccatcgatca cgcacgcggt cggctcgatc agaaagaacg
     8281 ccgtgccgac gccatccgcg atcacgacgc ccgtcgggca atgatgatcc ggcgtcggat
     8341 tcttcgggac ccgtgcgccg atgaacgaac agccgctcat gagccacaac accatcacgc
     8401 atgcgcgcat ctcgggccct agtccgtcag cttcttgatc acggtcttga tggcctcgac
     8461 ggcatggtcc gcatcttcga agccagcgcg cgctttgtcc ttgccgagcg cctcgatcga
     8521 gcgactaatt ttttcgcgac gcgtgaccat cagatccaga aactcggcga tgtccagctc
     8581 gatgagctcg gcgacgtcga gcacgatcgt gccgttcggc gacttgcctt cgtgatagcc
     8641 gcgccgcgtg agctcggcga gcagcagttc cttgacgaac ggggtcacca gcagtcgtcc
     8701 gagagctcca tgagatcgag atctgactgc tcgatcagct ttttcgcaag cgaaaggcca
     8761 ggcagcacgt tcttcgcgta gaagcgcgcg accgcgacct tgcctttgta gaacgcgacg
     8821 tcgtcccccg tggcggtcga tagcttgccc agcgcgacct tcgcgttgac cgcgagacgc
     8881 cagccaatga cgagctcggc cagcgcgaac aggatccgat tgccttgcag gccgacgtga
     8941 tagacggact ggcccagctt gcccatcatc gtcatcatga tgccttggca gtctgcgacc
     9001 gcttgctgga gcgcggcctt ctccggcgcg agctcggccg gcagtccatc gagcgtcgcc
     9061 gtgatctgac cgacgagcgc ttgcagcgtc gcgccgcgat cgcgaccgat cttgcggaag
     9121 aacagatcga gcgcttggat gtgcgtcgtg ccttcgtaga gcgaatcgat cttctggtcg
     9181 cggatgtatt gctcgatcgg atagtcctgg cagaagcccg agccaccgaa gcattgcagc
     9241 gcgacggcga gcagctcgta gaccttctcc gagccgtagc ctttgatcat cggcagcatg
     9301 aagtcgttga gggcgtcgag cggcctcgct tcttgattgc cgtgaccacc gcgcagcgcg
     9361 acttggtctt gcacgttcgc ggtccacagc acgattgcgc gcaggccttc ggcgaacgtc
     9421 ttctgcagca tgagcatgcg gcggacgtcg ggatgcttga tgatctcgac gcgcggcgcc
     9481 gacttgtctg ctgccttggt caggtcggcg ccttgcttgc ggacgcgcgt gtagtcgagc
     9541 gcgttgaggt acgccgtcga cagcgttgcc atcgacttcg tgccgacgcc catacgcgcg
     9601 tactcgatga cgtggaacat ctgcgcgatg ccgtcgtgga cttcgccgag cagcaggccg
     9661 cggcacttcg cgtcggcgcc gagcgtcagc tcgcaggtgg cggagccgcg gatgcccatc
     9721 ttgtgctcga cgttcgtgac gacagcgccg ttgcgctcgc cgagcgaccc gtcctcgttg
     9781 acccagaact tgggcacgat gaacatcgac aggcctttgg tgcccggacc cgcgccttcg
     9841 ggacgcgcga gcaccatgtg gatgatgttc ttcgaatggt cgaagtcgcc gttcgtgatg
     9901 aatcgcttca cgccctcgag ctcgtacacg tcgccttcga tgtggcgagc cttcgtcgtg
     9961 cctgcgccga cgtcggagcc cgcggccggt tcggtcagca ccatcgtcgc gccccagtgc
    10021 tggtcgatca tcggcttgat gaagcgcgcc ttctgcgacg gcgtgccgaa gttatcgatg
    10081 atcgtcgaca tcacgttgcc gagcacatag aacgtgaccg acgggttcgc cccgctcatc
    10141 agctcgaacg cggaccagcg cacgctcgga ggcgcgccga atccgccgag cttctcgggc
    10201 agctcgatgt tgttccaacc gccgttgaag tacgcgtcga gcgccttgtg ataggcggcc
    10261 ggcagcttga cattaccctt gccgtcgaac gtcgcgcctt cgcgatcgcc gggaacgaac
    10321 gccggtgacc aggtcgtctg cagcacctcg aggaagccct cgagcgccgc gcgcgctgag
    10381 tcttcgtcca tcgactcgaa cggcgccttg ccgaggaccg tcttctggat ctcgaggacc
    10441 tcgaagaggt tgaagaagat gtcgcggagg ttcgggcggt aatgcagcgc ggtcgagctc
    10501 atgaccgccc cactatatgt cagcagccgg tcgtcgtctg ctgcgccagg ttcagcgtgc
    10561 gctgcagggt cttatcgagc ggcggcacct gcacgctgtc gttgttgagc cagcacgccg
    10621 tcgagccgac gaagcccgtg atgtgcatcg tgtagccgtc cgaggtgacg ccgctggcgg
    10681 taattgtctg atcggattcg atgcagccga atgtgaccgg cgtcgtgcag tcgatcgtgt
    10741 acgtgccgcc ggtctgcgtc gcgccgttgc tgatcgtcag cgtgatcggc gcgcagctcg
    10801 tgtcgctcgt gtggttcatc gtgatcgtga cgccggtgat gcccgcgccg ttggtcgccg
    10861 cgccgcagtt gccgcccggt ttgccggttg cgagcttgag ctccatggcg cccgtcgctt
    10921 ggacctggaa cgtcagcggc gcgagcggcg tcggaccgcc cggcggaacc gcgacattgg
    10981 tctgcgccgt ggccgtcgcg aggagaccga acatcgcgct gctcagctcg aactgaaaat
    11041 cgtagttgcc ggcgatcacg gcctggctcg tgcccatgcc ggtgccgcac gtgaagatct
    11101 gcgtcgatcc gccgtcgaac gccttgttgt gggcgagcac ggtcatcgtg ttcccgccga
    11161 tgcgctcaca cgacagcggc tggttgttct gatcgatgac ggaccaggtg gcggagaacg
    11221 tccccgtggg tgccgcgtcg agctccttcg cgtccgggaa cccaccaccg ccgccgcacg
    11281 cagcgagcag gcctgcacag acaaagcgaa gcgtcgatct cattcagcgc atcgtaacac
    11341 tacgcggccg cagccttcac ggccccgagc cctgtgacgg agcgcaaact cttgcgcatc
    11401 tgcacgaagt ggcagggctg tttgacggcc cgatgctcgt acggcttggt cccgatctcg
    11461 acgtagccga gcgagcggta ccagcgtgcg agctcttcgc gcaggttgat gatttgaagt
    11521 gtcatctcgt cgcagcccgc cgcctcgcac atcgcctcgg caatacgaac gagtcgcttg
    11581 cccaagccgt gcttctgcat ctcgggcagg accgacagca tgccgaagta gccgcgctcg
    11641 cgcgacgtct cgacccagac cgccgcggcg agaccgcccg cgtactcgag cacgatgaac
    11701 gtgcccttct cgatcaggcc cgcaatctcg gtcgcgttcg tacggtcgcc ttcgacgaag
    11761 aactcttcga tcgaataggc gcgattgacg agcgccgcga gcgctgaggc atcggcagcg
    11821 gtagcacggc ggacactgat agctagatcg gtctgcacgt ttgatcgcgt accagaagcc
    11881 tccgacatcc agcgcgcgat cacaactctt cgaccgctct tcgaccgctc tggcgacagg
    11941 cgggatcgga tcgtcagtgg tgatacggcg cggtcggtgc gggcgcgagc tgctgcgact
    12001 gcgaaggatg caaacgacgc gtgcgagcgc gtgtcctatc ggcaagcgcg acatgaacca
    12061 gaacgatcac cgttccgacc gtcacgtccg acaccgcctt caccgttcta tcgaccaagc
    12121 tccacatgtt cgtcccaagc caatctgcct catcaacatc ccaacgacaa cacgtatttc
    12181 cggctctgga acgatgtgta gaaatccgtt ggccagctcg cgcgttcata tcgctgtgag
    12241 gtgcctcctt ctattggcag cagcgtgcac gcaagcagcg agcgcgccat ctcctccgca
    12301 aagcgcgcca cggagtgtga tccaaacgca cgctccgatc gctgcgccgc agcgcgttgg
    12361 tgacgcgacc gcaaagggat ccgtcaccgg tgtgcaaatt ctcgcgtcgg tggacggcgg
    12421 cgctgcgagc gatcgcccga cgtacgcacg caaggatcag aaggtgacgc tgtacgcgct
    12481 cgttcgcgtg ggagcggcct tttacaccga tgcaccatca ccgcacctcg ccggcaaagc
    12541 gatctccgct cagccgctcg cgaaggcgcc ttcgatcgat ctcgcgtggc agcgcatcga
    12601 gccgacgact gcgaacatgt cgaacacgca gagcggctcg ttcaagttcg aagcgatcga
    12661 gtacacgacg agcgcgatcg ccgccgcaaa tcagccgtcg atcaccgcgg acgtgcggcc
    12721 gacgctcacg accgatcatg gggacggcat cggcacgatg cgctaccagc tcgtcgcgac
    12781 gcagggcgac cgcgtgctcg cgagcccagg catcgatgcc aagcgcggcc gcggctcggg
    12841 tggtctcggc gacgcagtca cgcgcgtcac gattcgccgc gacgactcgt tcctcggtta
    12901 cctcaccgag atgtacggcc agccatatat ctgggcctcg gccggtctct ccgatggcag
    12961 tcacgagagc gagcacctcg aaggctcgga ctgcgcggac ctcatggtct acggcgcgcg
    13021 tcgcatgggc aagaaggtcg cgtacacgtg gaccggcggg ctcccaaacg tgacgaagct
    13081 cgtcgcggga agcggcacgc gcggagacga cggcatctat cgcgacaagg ccggcaagcc
    13141 gttgcccttc accgccgtcg gcgacctcgt gctgttcccg cgccacgtcg gtgcgctcgc
    13201 cgaagaccgc ggcacgatcg gcgtgctcga cgatcaagac atcatgctgc acacgctgtt
    13261 cgactcgccg aaggaacagg cgatcgcgga cagtggctac gccgatacgg ccgtcgagct
    13321 ccgtcgctgg aagtagctac acgaggtctt cgtcgggatc gacgatgaac gcgcagaggt
    13381 tcgcgagcgg tgggtgaaac gccgtcgagc caaacgggag aagcgcgccc acttcatcgg
    13441 cgtgcagcat ctcggagagc ggcacggggc gcgcgatgac gccgaccgcc gcaccgccct
    13501 tgacggcatc gagcacgcct tcagcgtcgg tgctcgtcgt cgataccgtc tgcagcacgc
    13561 gccgcatgaa caggtgctcg atgacgaccg ggtcgtagcg ctggatcgcg cgatggatgt
    13621 cgatgccctc gccggcgagg ctgacgtcgg gcttgagcgt gagcttgtag gcgtccgcat
    13681 ggcttgcgaa cgtcacgaca aacgcaggtt gatgcgcgat gtggtcggcg agcgaggcct
    13741 gctgcttgcc gagatcgcga gctgcgcctt cgagcttgtc gacgacgaag tagcgcttcg
    13801 ccgcttcgag cacagcctcg cgcttgagac cgtcgttgcg aacgaggcga tggcgcggcg
    13861 cgaccgcgag cgccggatcc tcgaggttca cgaggcagcc aaggccgtag ttcgccgacg
    13921 agtactgcgg ctgcttgtca gccccgagct tggcggcata cgcgagcatg ccttcgtagc
    13981 gcgcatggcc gtcgagcacg tgcagcttct tcggtgcgaa tagcggtcgc agcttgccga
    14041 tcagctcggc gctcgagatt cgccacaggc gatgcaccgt cttgtcgggc gtcgtcgctt
    14101 cgagcaccgg gcgatcgcga tcgatgttgc gaaggaggcg atcgacctcg cgcgccgcat
    14161 cgcgatagcc ggcgaacacc ggatcgcagt gcgcgccggc ctcggtgatc gcagcaatcg
    14221 ccgcgtcgcg cgccgtcggg ttcgtctcct cgtgcgggca gatcagcttg tcgctccacg
    14281 gcgagagccg gatcgcacag aggagcgtcg tccgcgtcag cgtgcgcgcg ccggcgtcga
    14341 acgattggtc gtagcgatac atcgcgcgcg tcggatcgcg aacgagctca ccctttgcga
    14401 ggcgatcttt gacatgcgtc atcggctcgg cagagacctt cttgaggtcc accttcgacg
    14461 catcccagag ggctccccgg aaccctgcaa tctgcggcat gactcgaatg tagcctagct
    14521 gcgagcgccg atgtcgcgac gtgcctgctt gccgggcagc tcgatcgcgt cgacgacagc
    14581 gtatgcacgc tcacgtgcct gctgcacgct gacgccgcgc gcggttatgc cgagcacgcg
    14641 cccgccggag gtcacgatgc ggccatcttc aggccgccgc gccgtgcccg cgtgaaagac
    14701 cgtgacgtcg tcgggaaccg cgtcgagacc gccgatcgga tcgccggtgc ggacttgccc
    14761 gggatacccc gcggccgcga cgaccacaca caccgcgacg cgcgcatccc acgcgagcac
    14821 gccatgcggc agctctccac gagcagcacc ggcaagcacc gacgcgaagt cgccgaccat
    14881 gcgcgccatg atcggctgcg tctccggatc gccgaaccgg cagttgtact cgagcaggta
    14941 cggcgcgccc attggatcga ccatgacgcc ggcgaacagc acaccgcggt aatcgatgcc
    15001 gtcgcttgcc agacctttca cggtcggctt caagatctcg cgcgtgatgc gctcgcacac
    15061 atcggtgctc acccacgcgg gcgagaccgt tcccatgcca ccggtgttcg gccccgtgtc
    15121 tccgtcgaag atcgttttgt gatcctcgac ggcgggcagc acttccaagg tcttgccatc
    15181 ggtgatcgca aggacgctca cctcgcgacc gacgatgcgc tcctcgatga tcacggtgcc
    15241 gccggcatcg ccgaagcgct tcgcctcgag catgtcgcgc gcggcagccc gtgcctctgt
    15301 cgcatctgct gcgacgacga cgcctttgcc ggcggcgagg ccatcggctt ttactacgac
    15361 ttgcccaccg agctcctgga tggcgcggtc tgcgtctgtc acgctgttga cgacgaagaa
    15421 gcgcgccgtc ggaatgctgt ggcgcgcgaa gaactgcttg gagaatgcct tgctgccctc
    15481 gagtcttgcc cccgatgcgc ccggaccgaa cgtcagaatg ccgtgcgccc tgagctcgtc
    15541 ggcgatgccc gcgacgagcg gcgcctccgg accgacgaca acgagatcga cggcctgctc
    15601 tttcgcaagg cgcaccatgc cggcgtggtc ggtgagcgga acgggaaaac acgcagcgat
    15661 ctgctcgatt ccgggattcc ccggtgccgc gatcaccgtg ttgcccccct gcgcgagacg
    15721 ccagacgagt gcgtgctcgc gaccacccgt gccgagaacg aggatcttca tgcggggcgg
    15781 aatctagcag cgcaaccatg gggcggtcgc gttgtcagaa gcacatgcga cgggtcgcgc
    15841 tggccttggc gacgtccttg gctacgctcg ggagtgcgca ggcgctggcg caaccggccg
    15901 gcgactgggg cgtgaagcgc gacccgttcg acctcacggt tgtcgcacgc taccgcgcga
    15961 tcctcgcgaa gaatccgtac gacgtcggcg cgctcgacca gctgcatcag atgtacagcc
    16021 gctatcgcac ggcgtacgag ctcgagaaga gctactcgag cagcaccgac tgggcgacgg
    16081 cggtcgtcta cgcgcggatc gcgaaagacg atatcgaccg ctggaagaag gtcgtcgagc
    16141 tcaaagcaga tgatgcgcgg ggtctcaacg ctctcgccga tgccgagcgc ggccagggcg
    16201 cgaccaacga agcgttcggt cactaccaaa aagccgcgca actcgcgtcc gacaacgcga
    16261 cgaagaagaa cgcgctcgag tggaccgcgc ggctcgccga ggacttccag aacatcacga
    16321 ggctcgagtg ggcgtacgcg cagctcatcg cgctcgagcc gaaggagccg aagcactgga
    16381 ccaatcgcgc gctctcgatg cagaagctca atcgctacac cgacgcgatc gagtcgttcg
    16441 ccaaggcgga agcgctgacg acgacggatc cacaaggcaa gctcgacctg atcctgctcc
    16501 gcgcccgcgc gctcgtgcag atcggcgata cgcacgccgc gctcgagcaa tacaaccgcg
    16561 tgctccagtc cgcgccgcgc ggctactacc tcgccgatga agcgatcact gcgctcatcg
    16621 atctccacct cgctgccggc acgctgcctg aattccgcct cgcgctcgag aagcgatggc
    16681 cgcccggcat gcgcgcgtat cccgagtgga agacgctcgc aaacgtctac gagcagctcg
    16741 gcgatacgga aggcgcgatc ttcgcgctca agaaagcggt caccgctgct ccgtccgagc
    16801 gcgacacgca cacgaagctc gtcgagctct acgacggcaa tggcgaaacc gacctcgcgc
    16861 tcgcgcagct cgaagccgcg acaaaggcgc tgccagccga ccaagacttt cacgtccaac
    16921 tcgccaaccg ctaccacgac gcgaaagaga cgaagaaagc gttcgcgacg ctgcagcgtc
    16981 tctcgcagac gattcgcacc gatgctcgcg tgcacgaaga cctcgcggac ctctacacga
    17041 agtggaagcg accgggcctc gcgctccacg agctcgaaga aatcgcccgc atcgagccgg
    17101 agcccgagaa catcgccaag ctcggcgagg cgtattggca aggcggcagc gaggacaaag
    17161 cgctcgcgac gtggaagaag ctgggcagcg atccggaggg tctcgcccgg ctcgcgcagg
    17221 tctacgtcga tcacgagttc tggcccgacg cgatcgtcgt ctacacccaa gcgctcaagc
    17281 tcgcgccgaa gaacgccgag ctctggcgcg cacgcgccga cgtccacgag acgctcgagc
    17341 attgggagga cgcattcgcc gacgcgacca aggccgccac gctcgtcaaa ggccgcacgc
    17401 tcgacgaagg tcaccaagtt cgctaccagc tcgtccggct actctcgcgg tgggcggagc
    17461 acgagaccga cggcgagaac acgcacgagg ccgccgcggt ccgtgcgtgg acgcgcgact
    17521 tcgtcgccga accgcccgat ctcgaagcgg gctacatgct cgccgaatac gaaggccgct
    17581 cgccttccgc cgactcgctc gccgcgctcg aacgcctgca caagctcgtg cccaccgacc
    17641 tcggcgtcga gcaaacgctc gcgcgcacgt acaagatcct tcaccgctac aacgaagcga
    17701 tcgcgcagct cgaatcgctg acgctcgccg atccgaacaa ggccctcgac taccaagacc
    17761 aaatcgccga tctcaaaaag cgcatcgacc tcgacggccg cgaaggctgg cgcgacgacg
    17821 gcatcgccga tcccgacgcg tgggtgcgcg cgcatcgcaa gcccgatccg atcgagccat
    17881 tccgcctcgg cctgcacatg ggtgtcggca atgcgatgcg cggtcccgct ccgcacagcg
    17941 tcctcgtcgg catgtacacg tcgattcgca tcggccgtcg cgtcgcattc gtcggacgct
    18001 tcgattggtc cgagcgcggt ggcgacatga cgtccgtcaa atcagccgcg cttgccacgg
    18061 gcgtcgccct ccccgtcctg aaccttagcc gcatggtcgt cacgctcggc gcggcgcacc
    18121 gcaccgagct tcgcatcggc gatcacatgg gctgggaccg cctcggcctc gccggcgact
    18181 tcacgctcga gctgatcccc aagaagatgc cgggctcgct cgcgattcgc ttcgagcagg
    18241 gcctgtcgga tcacggtcag tcgaccgcgc tactcgtaga gctcggcgtc ggaaactgat
    18301 acgccgtagc tcgggtatga agaagcttgt cgctgcgctg gttgtggtgg ccgcggtcgt
    18361 gatcgcgttc gtgatgtggc cgcgcgagcg agcgcatccg aagtcgacag cgccggcgaa
    18421 tgcagcgggt agcgcgacca cggctgtcac gtttcgcgga accgacggag tcgagcgcac
    18481 gcttccggcg tggatgttgt cgccgggcgc accggcgaag cggattgcag ggcgcgtcat
    18541 atttcgcggc gaaccggcgc gcgacgccat cgtcgagctg cacagcatcc tcagctccac
    18601 gaacctcagc gccgcagtcg ttgttcgcac ggacgcgaat ggccacttcg atttcggcgt
    18661 gcggccggcc gctgcttatg acgtgactgc aacgcggccc gatacgacgg ccgccatcca
    18721 gcatgtcgag ctcgcgaacc caacgctgac tccggcgtcc gatcgactcg agcttgtcct
    18781 cggcgagtgt acgaattcca tcgaaggcac cgtcgtcgac gcgtcgaacg atccgctgcc
    18841 caaggcacgt atccgccaga acggttttgt cggaccggag accgacgcaa agggccacta
    18901 caagctgtgt gtcgcgttcg gcgatgcaga gctgcactac tccgccgatg gctacggctc
    18961 tgtggttctc acgatcgacg cgcgcggcca gatgcgacgt gatgtcgtgc tcgttcccga
    19021 agcgaccctc gatgtgacgg tggtccgcga ggatgatgcg aagccggtcg aaggagcgct
    19081 cgtcgtcgcc ggcccgatgc aatggggacc tgaccgagcg ggtccgtcgc gagggctcac
    19141 cggtgccgat ggccgcgtac ggctcggcgg gctcgtgccg ggtcgttatc acatcttcgc
    19201 gctcggcgat gatgcgcaga gctcggctcc agctgaagcg ctcgcacagg tcggtgttgc
    19261 gacggacgtc gtattgaagg tcgccgctac ggcaagggtg actggccacg tcgttagcgg
    19321 aacgaagccg gtcgcgggcg ccgaagtcac cgcggttcgc aaggcgccga tgggacggtc
    19381 gccggtcgtg ttctcgcagc tcgacggttc gttcacgctc gaccgtgtcc cagccggcga
    19441 catcagtttt gtcgcggctc cgtacgaggt cgagtcgcct gcgtcgatcg cggcagcagc
    19501 cggcaaatcg gtcgacgtca cgatcaaggt gcatgcgctc ggcgcgattc gcggccgtgt
    19561 gctgcgcctg ggcaaaccgg tgcctgacgt gcgggtgtgc tgcgtgcaaa cgatcgccgg
    19621 tcttcggccc gatgcaatca cgcgcgccga cgggacgttc gagtatcgcg gagtgtcggc
    19681 gggcgagtat gacttgcaag gcggcagcga cgagctcggc gcattcaaca tgccggtgca
    19741 cgtgaagctc gcagcgggtg aagaaaagtc cgtcgatctc gatctcgata tggcgggcac
    19801 gatcaccggt accgtcgtcg ataaaagcgg tgcaccggtt ccgagcgtgt tcgtccgctg
    19861 ggtccaccag acgtccggcg acctcggccg ctgcatgaca aacgcaaagg gcgagtatcg
    19921 ctgcggcacg atgaccggcg gcggcacgta tcgcgcgggg gtgtttccgt cgtccgagct
    19981 ccagggagtc ccctacccga cggcgacggg cgcgccctat ccgagcgtcg atgttgcgga
    20041 tgcgaagacg ctcgtcgagg gcgtcacgat cgcgatcgat cacgcgcaac aaacaatctc
    20101 cggacgcgtc atcgatgaca caggcgcacc ggtcgtggat gtcatcgtca aggcgatgcc
    20161 gaccggtgaa gccgcgccgc agttcaacag ctggctgcgc ttgccgatga cctcgacgga
    20221 tgccgacggc tcgttcacgc tgaacgagct cgcacccggt cagtacgcgc tcgaggcgca
    20281 cgcggctgat ggcggtgaag gaatggtcac gggcgttgcc gccggcgcga agagcgtcgc
    20341 gatcaagatc gagcgcgcag gcagtatcac gggcacgctt gtcggattca cgaagccacc
    20401 ggtcgtctac gcgacgccgc agggtccatt caagctgttc accgggaccg tcgacgggca
    20461 gagcttccgc gtcaccggct tgcggcccgg ccgctatacc gtgaacgcgc agaccgcgat
    20521 cgaaggcgac atccagcagg tagatgtcaa agctggccag ccggcgaagg tcacgttgac
    20581 ctcgcacggc aagggaacga tcgaaggcac ggtgttcgag ttcaggacga agaagccgct
    20641 cggcggcgca acgtgtcgcg tcgtgatgca gatgaatggc gagcagtcgc aaacaagctg
    20701 ggatccagca ttggcgccga agagcgatgg cggcggtcgc atcgtcatcg acccggcgcc
    20761 ggccgggccc gtgtcggtga tctgccagcc gatgggcttc aagtggtcgc tcgcgacaac
    20821 cgacgtgacg ctgaccgcgg gtgcgcgcgc gactcctcag ctcatggcgg tcgagctcac
    20881 gcaagacaac ccgacgtcga ccggcgcgca gctcgattgg cgtgttacgc caccacggat
    20941 cgctcgcgtg atgcccaaca ccccggccgc agcagcgggg cttcttgcgg gcgacctcat
    21001 caccgcggtc gatggcgtct ccgtcgcagg tctctgcggc tccgccgtct cggtgctgat
    21061 ctcgaacacg gccgcaaaca gcgacgtgaa gctcaccgtt cagcgcggca cggcgacgaa
    21121 gaccgtctcc gtcaaagcgc tgcacatgcc cgactgaatg gtagcggggg tgggcgggat
    21181 agcccgcgga actgggcgac ttcgcccatc cgcgagcggg tgcgttggaa cggcgattgg
    21241 accgcgatta ttgcggtccg agattcgctc gtggttcatc ggcgaggttc ctgcaaaagg
    21301 gcgcgccatg aagatcctca atgtcgttgc tccgtgtgtc tgcttgctgt cgtttgccgg
    21361 ttgtgcggct gattcgaccg acatggggag tgtgacgatt tctacgtact cgctgcacgt
    21421 cgcacgcgag ggtgatggcg cggtcgctgg tgccatgcct cgcttcgtga tcaagccgac
    21481 ttccggcgga aacccgacat cgatcaaggc ctgggtgggt ctggaagcca tcgacgcgac
    21541 cgggaagttc ctcgcggcgt acgacagtgc agatggcgac ttcgatgcgg acgtaactgt
    21601 tccgacgcca cttcccacgg gggcgcagct gtggctcgat gtggagacga ccgggaccac
    21661 aacgaccggc tcgatcgctc tcaagtgaga gaataggggc agatgatcac gatcaagatc
    21721 accaacgtcg aggacatcgt cgagaagaac aagggttggt tcgtcgctaa cgtcgtcggt
    21781 gcgttcgtcg atctcgagtc gcgcgtcgaa gacgtcatca tcacgaagct tcgggagtcg
    21841 ctcgctcgcg agggtgtcga agccgtgatc gagcgcgcgc ccagtcaacc gccgcgcgcc
    21901 ccctgaagtg gcgcgccgat cgcatacggc ctggcgaaca tgcgtgctgt gctattcgcc
    21961 gttctcttca cattcggatg cagctccaac aaggagaatg ccaacccgtc accttcggca
    22021 tctgctccga cgtcggaccg acgtgcagac gacagggcaa ccgaggcgga agagagtcgc
    22081 cacgtcgcaa aggtagaagc tgcgacggcc aaggaagcgg cagacgtgaa gctcacggcc
    22141 gcgcatgatg aaaccgccgc tcgactccag aaggaatt
//
LOCUS       DATLBZ010000101         2333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9028460, whole genome shotgun sequence.
ACCESSION   DATLBZ010000101 DATLBZ010000000
VERSION     DATLBZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9028460"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VMZ53_05330"
     CDS             complement(<1..498)
                     /locus_tag="VMZ53_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HUS27904.1"
                     /translation="MTTNSFSSKAQIPVGSNKIELYRLDALVKQKVGDVETLPYSLRI
                     LLENVLRYEDGKTVQAADVQAVASWNPKQRVEHEVQLRPARVLMQDFTGVPAVCDLAA
                     MRDAFVALGLPGNSINPLNAADLVIDHSVQIDEYGTNLAFRKNVELEYQRNRERYLFL
                     RWGQQA"
     gene            473..1783
                     /locus_tag="VMZ53_05335"
     CDS             473..1783
                     /locus_tag="VMZ53_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HUS27905.1"
                     /translation="MLEKLLVVMCLDASRGESNRSLALRWGVALVTEPADVAAIAAEL
                     SRAPLVAFDLEFLSQDRLVPTLCLLQVSWLPANIGLDAPAGQIVAVEPPIRLIDPLAV
                     DVRPVIEALAAHPLVVAHAPRQDLGLLATRFKVSMPGIVDTQLMAAFTGIGDQVGLAA
                     LANELLGTSLGKDQQWTAWERRPLSDAQITYAAADVRYLPALYALLAEKLGPRIAWVR
                     EESRAVLDDALAASAVTPETAWENVGGARYLDAASHAALVAMAAWRQQTAIDMDRPLG
                     QVLNEKTLVELAKSRPDTANEIRGIKGISPIAKTRAQEIVDALAVARTAPAKPAAPRP
                     PSRAASPRAQRWSELLLAIVQLVSDETGIAARLLGTRSDAEAFARTVDEHGLDAAKSL
                     PALSTWRREVLGQVWEGWLAGTVALRGDTETAQGMRLVWKAPPA"
     gene            complement(1780..2244)
                     /locus_tag="VMZ53_05340"
     CDS             complement(1780..2244)
                     /locus_tag="VMZ53_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27906.1"
                     /translation="MRNTLLHRNDDLETAGPSHKVSAATRRSELATDAGTRSGPAIWE
                     AASLDRDEPSTSSVMHAGQRQLSPDVEQSLISIVMRPLRESESHHTGNENREQEVRAL
                     FAELSPLDALLLRRRLDANRTEDPVVIAFRRLVVDRRQRLYGYLADPRRGAI"
BASE COUNT          403 a          831 c          702 g          397 t
ORIGIN      
        1 cgcctgctgg ccccagcgga ggaacaggta gcgttcgcgg ttgcgctggt actcgagctc
       61 gacgttcttg cggaacgcca ggttcgtgcc gtactcgtcg atctgcaccg agtgatcgat
      121 cacgaggtcg gcggcgttga gcggattgat cgagttaccg ggcaaaccga gcgccacgaa
      181 tgcgtcgcgc atcgccgcca aatcgcacac tgccggcacg ccggtaaaat cctgcatcag
      241 tacgcgcgcc ggacgaagct gcacctcgtg ctcgacgcgc tgcttgggat tccagctcgc
      301 gactgcctga acgtccgccg cctgaacggt cttgccgtcc tcataacgca acacgttctc
      361 cagaagaatg cgcagcgagt acggcagcgt ctcgacgtcg ccaaccttct gcttcacgag
      421 cgcatcgagc cggtacagct cgatcttgtt actaccgacg ggaatctgcg ctttgctgga
      481 gaaactgttg gtcgtcatgt gccttgatgc ctcgcggggc gaatctaaca ggagtcttgc
      541 gttaaggtgg ggcgtggcgc tggtcaccga gcccgccgac gtcgccgcca tcgcggccga
      601 gctctcacgt gctccgctcg ttgcgttcga cctcgaattc ttgtcacagg accggcttgt
      661 accgacgctg tgcctgcttc aagtctcgtg gctaccagcg aacatcggct tggatgcgcc
      721 cgccggacaa atcgtcgcag tcgagccgcc aatccggctg atcgaccctc tcgccgtcga
      781 cgtgcgcccg gtcatcgaag ccctcgcagc gcacccgctc gtcgtcgcac acgctccgcg
      841 ccaagacctg ggcctactcg ccacccgctt caaagtctcg atgccaggca tcgtcgacac
      901 ccaactgatg gccgccttca ccggcatcgg cgatcaagtc ggcctcgccg cgctcgccaa
      961 cgagctcctc ggcacgtcac tcggcaaaga ccagcagtgg actgcctggg aacgccgccc
     1021 actctccgac gcccaaatca cctacgcggc cgccgacgtc cgctaccttc ccgcgctcta
     1081 cgcgctgctc gccgaaaagc tcggtcctcg catcgcatgg gtccgcgaag aatcgcgcgc
     1141 agtcctcgac gacgcactcg cggcctccgc agtcacgccc gaaaccgcct gggaaaacgt
     1201 cggtggcgcc cgctacctcg acgccgcatc gcatgctgcg ctcgtcgcca tggctgcctg
     1261 gcgtcagcag accgcgatcg acatggaccg accgctcggt caggtcctca acgaaaagac
     1321 tctcgtcgag ctcgcgaaat cgcgccccga caccgcgaac gaaatccgcg gcatcaaagg
     1381 aatctcgccg atcgcgaaaa cgcgcgcgca agagatcgtc gacgcgctcg ccgtggctcg
     1441 caccgctcca gccaagcccg ccgcgccacg cccaccatct cgtgcagcaa gcccgcgcgc
     1501 gcagcgctgg tccgagctcc tcctcgccat cgtgcaactc gtctccgacg agacaggcat
     1561 cgccgcacgc ctcctcggca cgcgctccga cgccgaagcc ttcgcccgca ccgtcgacga
     1621 acacggcctc gacgcggcga aatcgctccc cgcgctgtcg acatggcgcc gcgaagtcct
     1681 cggccaagtc tgggaaggct ggctcgcagg caccgtcgcg ctgcgcggcg ataccgagac
     1741 ggcgcaagga atgcgtctcg tctggaaagc gccgccggcc tagatcgcgc cgcgtcgcgg
     1801 gtcggcgagg tacccgtaca agcgttgccg gcgatcgaca acgagccggc gaaatgcgat
     1861 gacgaccgga tcttcggtgc ggtttgcgtc gagtctgcgc cgcagaagga gcgcgtcgag
     1921 cggtgaaagc tcagcgaaca acgctcggac ctcttgctcg cgattctcat tgccagtgtg
     1981 gtgactctcg ctctcccgca gcggccgcat cacgatcgag atgagtgact gctcaacatc
     2041 gggcgatagc tggcgttggc cagcatgcat gactgagctc gtggagggtt cgtcgcggtc
     2101 gagcgatgca gcttcccaga tcgcgggccc ggaacgcgtg cccgcatccg tggcgagctc
     2161 gctacggcgc gtcgccgcag agaccttgtg cgatggacct gccgtttcga gatcgtcgtt
     2221 gcgatgcagt agcgtgttcc tcatggtcgg tgctatcggc atcacccggc gtcggttgcg
     2281 tgacgcctac tgtcgcgtaa cgactcatct cgccggcacg caaccctggc ggc
//
LOCUS       DATLBZ010000102        23226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6008887, whole genome shotgun sequence.
ACCESSION   DATLBZ010000102 DATLBZ010000000
VERSION     DATLBZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 23226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23226
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6008887"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..5375)
                     /locus_tag="VMZ53_05345"
     CDS             complement(3..5375)
                     /locus_tag="VMZ53_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS27907.1"
                     /translation="MSVATADVLDAFHPLVGGWFRDRFGGPTAPQREGWPRISAGKDV
                     LIAAPTGSGKTLAAFLACLDELVRRGMTQPLPNHTSILYISPLKALSNDVHRNLEAPL
                     AELRERAAIEGTAFPDIRVGVRTGDTPMGERAKMAKKPPHILVTTPESLFILLTTQSG
                     RAALGQLKTIIIDEIHAIAGDKRGAHLALSLERLDRLVTQVSGRAPVRVGLSATQRPI
                     ERIGRLLVGTHRPMPHIVDAGHRREIDLAIEITDDELGAVASNEQFDRVYDRIAELVT
                     QHRSTIVFVNTRRLVERAAAALEQRLGEEHVVAHHGSMSRELRHNAEQKLKYGQVKCA
                     VATASLELGIDVGAVDLVVQLGSPRSIATLLQRVGRSGHTLGGTPKGRMFALTRDQLL
                     ECAALIRGVRSGNLDQIELRDAPLDILAQQIVATCAAEDIEEAELMALVRGATPYAAI
                     EDARLEQLMVMLSEGVSDRRGRTGAYLHRDRVAGMVRARRSARLAAITSGGAIPDNNN
                     YTVVQWPEETQVGTVDEDFAIDSSAGDIFQLGNTSWKIRRIEAGRVIVEDARGQPPTI
                     PFWFGEAPARTRELSDEVSALRGEIDQRLATESMDALAAWLAESCSMPLRAAQQAVAY
                     LAAARAALGALPRKDLIVAERFFDEAGGMQLVLHAPLGGRINRAWGLALRKKFCVTFD
                     FELQAIATDDGIVISLGQPHSFPLDTVFGYVPSHQARETLIQALLDRPMFEIRWRWNV
                     TRSLTVLRRRAGKKIPPHLIKMRAADTLSVVFPQAQACAENIVGPREIPDHPLVFETI
                     RDCLVEAMDLEGFRTLLEQLERGEIRVMARDTVEPSPLSHELINANPYAFLDDAPLEE
                     RRTRAVQLRRGLPASITTDGVDNVGALDEAAIELASHEVAPTVRDADELHDALLALWL
                     VPEHLGRTWGDSAGTVDDWFAALADTGRACRLRWQAPATAAAAADRTCVAWCATERLG
                     AARALLGADVECTPMIATPTWAQTPEREVALNKLVSAHLDHRGPVNTRALADELGLPL
                     PEILGALLALEGDGAILRGTFTSASTAARKTVREALTAADDSDLLADTEWCNRRVLAR
                     IHRLTLARLRKEIEPVSAAALMRFLLRWQRVAKNTQLIGLDGLARVVEQLQGFETAAG
                     AWEREILPSRIYGYDPSWLDQMCLAGHLVWCRLSPRVTLASEAEPLTTDEATKLLAAL
                     TQVASHRSELDAFPPILREPMQIGERAATRAVAQQAKSRKPANANRKLWGEPTWVARA
                     REQRSALDHAPASARKSSTAAHDGDLFDGDARAASTIGRDPFDRDDLDARAIDRDARP
                     GDRDARDGRAFDRDARTGDRDDRDARNGDRDDRDARNGDRDARAFDRDARTVDGGDAG
                     GARDARAHRERVVPEWIAPAVSDGVPAAVIQAAAEATLAAALDAGATRQQAAAGIAGM
                     LARKFPVLQALSIARVALETAFPEAPTRRNAPSRSAPLALMLRRDATWLRAAVAVANA
                     EPPVLSPTAARVRDHLVGVGASFLPDLVGGMDISPDAVEDALWELVGAGLATADGFAS
                     LRVLVDRKRGEVKSHFDRAAASAAAAAAAAATPVRKWQEAIKKARNRDRERPAHALRA
                     LPTAAGRWSLLADAKPEQLDAEASARQLLHRYGVVFRDLVARESSLPPWRDLLTALRR
                     LEARGEIRGGRFVTGFVGEQFALPEALDELRGVRNPGPAAEVARVAATDPLNLVGVLS
                     AGPRVPAIVGNAVLYVDGHPVASVEGGELVMRGPLPPGAHVDDDLTYHPPPRPASPAP
                     QAALPL"
     gene            complement(5437..6270)
                     /locus_tag="VMZ53_05350"
     CDS             complement(5437..6270)
                     /locus_tag="VMZ53_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS27908.1"
                     /translation="MRFLVSTTLWVAAIGSGCGFEHGQLENGDDSVDPQPDAGGTIVQ
                     PPGTGARTCAYPDQQLKLCLEFNDGVINPIVRDGSSMQLDAQAVGLKATMRTTTPPDP
                     AITMDASASLSIMETPTLDITNAITIEAWIRPTRYLASTIIGNQGQYRLTLNDAGFVG
                     CEMAGKDVHSFSNATSIPPGEWHHVACTFDGSQVRVFVDGSSRDCARDANPMISKTGT
                     NGTKIAPDFTGDIDGVRIYARNLGGTNMLGSTNELCAHANKTNCQRSCDSFGPGPGGD
                     W"
     gene            complement(6387..7022)
                     /locus_tag="VMZ53_05355"
     CDS             complement(6387..7022)
                     /locus_tag="VMZ53_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27909.1"
                     /translation="MLAALAADLAAAGFDLLHPFDARACAAETGARLDGGRLGLFVGN
                     TRALWPVFSAARANDPELASASHPLELYTERVIDHVIARVATSLPNARVYYGHRQYDG
                     AFLPFQRLAVAAGIGSLSPTQLVIHPVYGPWFALRAVITCDGEPPPRVAAPRACSCDR
                     DCLDAFSRAMSSTGPDVWRAWLEVREACTVGREHRYSDEQIAYHYSFLRIG"
     gene            7048..7857
                     /locus_tag="VMZ53_05360"
     CDS             7048..7857
                     /locus_tag="VMZ53_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS27910.1"
                     /translation="MPARLHHELIGSAPRRMLLTHGIYGAGSNWRGIARKLVAARPDW
                     SVVLVDLRNHGRSESGEPPHTLEACAEDLRALVAELGSIEVLAGHSFGGKVVLQARPL
                     VGDVAQIWMLDSSPSTRRDLANDPTSALAVLELLERLPRTWPSRDAFVQAIVDAGHTK
                     DLGAWLGMNVVADGDVYTLRLALPALREMLADYCARDLWHLVEDASLPGTVEIVIADR
                     SSTLSAADRERLSAPLAHIHAHHIDAGHWLHIDAPTAVVQLFAAALPTTLS"
     gene            8016..9044
                     /locus_tag="VMZ53_05365"
     CDS             8016..9044
                     /locus_tag="VMZ53_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27911.1"
                     /translation="MRIALCLVMLLTATATADDGDHTEKVRWRAPKGCPDARGLRQRV
                     EKRLERSLDDVSVGVDVDVEKRAGRYTATIDLRAVTVANDVRHLTSRRCGELADAVAV
                     IVARVASDALAHQDAARPPGDVIVVTPDAADAPTVADALPAARKTTAFAEEPTVPELR
                     QQVDSLSDGRYVPKRWAIGGRLSTVSAIGVLPRIGTGAELALTIRRQDHFAELAGTRW
                     IESSAQIYTGRPAKLDVGLDVAAVRFGWRPRNMPLRAWLGVESGSMRGTGVSLPTPQF
                     SSERWLAAGAGFGIAWQMRPWIRLFGANETMLAIERARFSNGQGMVLYAPNPMSFRTI
                     VGLELGWQ"
     gene            9041..9634
                     /locus_tag="VMZ53_05370"
     CDS             9041..9634
                     /locus_tag="VMZ53_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS27912.1"
                     /translation="MKFADGLVALAPPTSEMTALAHAIDAPAVPSFDEVYTAHFAFVW
                     RVLRTLGVPERGLEDAAQDVFVVVHRRLAEFEGRAAITTWLFAIARRVASSHRRATGR
                     DASRTEPLADEPAGGADTFAALSRAQASATVMGILETMDEDKRIVFALVELEQLSVPE
                     VARTLDINLNTAYSRLRLARQAFETAVRARAGRGVIP"
     gene            9631..10251
                     /locus_tag="VMZ53_05375"
     CDS             9631..10251
                     /locus_tag="VMZ53_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27913.1"
                     /translation="MTELSRSLLAAARDGLAPDPDVAARVRARVAAAVGAPLAKPVTA
                     KPVPSGGSGALLKLGALVVVAGVVTGGLALRRDARIESPRVSASTTDVDLEPAQSAST
                     QEHAAPPAAAKPARSVAPASLAREVELIDQAMSALRREEPAAALAFMRTFDRETLGHA
                     QMAEEAAAITIEARCGLGEDVAARIAAFDEAWPSSAERSRITDACR"
     gene            complement(10254..10886)
                     /locus_tag="VMZ53_05380"
     CDS             complement(10254..10886)
                     /locus_tag="VMZ53_05380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HUS27914.1"
                     /translation="MAKQRRTKTAGKTAAKKGPAGPTINVRHMMKRDIGAVWEFLKLS
                     FREVNAETVEYQRPRSRRRFEEVFDEEGTEQLLFEVDDEIVAYAEASYEVTGSDNWIN
                     PRYFEKRDMKPLFVDELAVHPDWHGKGVGSFVLEQLHHLARVHGLDHLVLEVAENNET
                     ALGWYRKREFKKLDAAIFMACEVVTEPELLPPRKLPTGSKPEPETTPDDE"
     gene            complement(10900..11472)
                     /gene="def"
                     /locus_tag="VMZ53_05385"
     CDS             complement(10900..11472)
                     /gene="def"
                     /locus_tag="VMZ53_05385"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HUS27915.1"
                     /translation="MAIRKIAQIGHPVLRQTAREVTREELASPAMQQFIDDLIETMRD
                     ADGAGLAAIQVYEPVRICAIEVRNNPRYPYKPQIPLTVLVNPVLTPVDDELFDNYEGC
                     LSVPNLRGKVKRNVHVRVQAWDRNGNAIDEVVHGLKAGTYQHEVDHLHGKIFVDRADP
                     STFTTWTEFERHHKSQFVEQVQQLVARVGS"
     gene            complement(11485..12612)
                     /locus_tag="VMZ53_05390"
     CDS             complement(11485..12612)
                     /locus_tag="VMZ53_05390"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HUS27916.1"
                     /translation="MYRLLYWLVLSRLPVEGTHRVSFALLRAFVAVPGMRALMQRVLG
                     PRDAALRVNVFGREFAGPLGLAAGFDKDAKGVDALLALGFGFVEIGTVTAEAQPGNPK
                     PRMFRLTKDRALINRMGFNNDGADAAAKRLSVPTRHGVVGVNIGKTKRVSEEQALDDF
                     ARSAKTLAPLAEYLVVNVSSPNTPGLRDLQAVDKLRPLLVAVRAACDMASPMRRVPLL
                     VKIAPDLADADIDAIADLALELGLDGIIATNTTIGRTGLATSEAAVTKIGAGGLSGAP
                     LKTRSLDVLRRLRARVGTRLVLVAVGGIESADDAWGRIRAGATLVQAYTGFIYEGPLW
                     PRRVQRELAAMVKAAGFATIEQAVGTDGAENPGAVASAAMR"
     gene            complement(12690..13430)
                     /locus_tag="VMZ53_05395"
     CDS             complement(12690..13430)
                     /locus_tag="VMZ53_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27917.1"
                     /translation="MFGSSLIAVAALVGCGGDDGKPVVPDAKKFMDAAPDAPHLCGVM
                     DSLGTLTFGSMAMPVVRQNFDVPTTGADMGKLEFFIAARLSMGTTPPIDALILDVIKP
                     TAGFMTNTPYAFETDATATGSIVADAMVFGDFDGSQTIQNLYWAQQGALTFTAFTGAG
                     APTAKDSKINGTVTMVPFKEIDQETGELMPGGCSTSIGGLTFFLQQSEVAPTPTVNRP
                     VEGSDPMLEAAIERYKGLKAARKLQQLQ"
     gene            13942..14589
                     /locus_tag="VMZ53_05400"
     CDS             13942..14589
                     /locus_tag="VMZ53_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUS27918.1"
                     /translation="MPSGPAEEGDLVGNVIVAAGEEKAWLGVHRHSDATHVKWPGGAI
                     PVEVPVDAPSRAFAKIEEAIAWAKLPIQAGHVALEIGAAPGGAVMALARRGVEVWGVD
                     TGELAPNVKAMPGVHHLAMKVGALRWEQLPPRVDWLLVDVNLAPQVAVHEVARLMPRL
                     SKTLRGCVFTLKLNELSFVPELPVLAERIAAFGFTDVRMRHLPSNRREVCCVALR"
     gene            complement(14586..18323)
                     /locus_tag="VMZ53_05405"
     CDS             complement(14586..18323)
                     /locus_tag="VMZ53_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27919.1"
                     /translation="MPLPYPHRATFLASTLVVPLAESASTPYVARMLALVVFDHFLRH
                     PVVTLGDDDERFGDEAGRLLDANHPQIEDTIDWLFRIARRHEVLWFEISVDRGRPQAT
                     LLRSRQPNGLNDEWTSSPDLSLSQQISQCLTQWLAARRLPLLASLPEFTHEDIRAAGD
                     RLVKAAAMLSLRNDYTTIPPKLLEPLPKMTVPYLRVLAEVSGAAARTLDPKVLELDPT
                     HPVARRNAYVSGLMRGAADRRDILPLVSECPMYGKPHLSIWGEPFDQDRPIENMGVRH
                     QGIAASLMPGNPYACHNYSLQLAEQARREESYRWADRATVAAPDFGAAHLDCVRRLRQ
                     VGRPGQAFAEAQYRCREILDRAQSGKLSANDWQAPHHAALLIAFAHLDVGRIQEAIEL
                     ADGALSQLPDDPATKDAFAWAHKRISHWKTDAGLLARAYAWEGYHRGDPGRAVLGLTR
                     GRITDDEDSAIMIESLNTLGREDQAEIAFHHCSGLDGNGLLADGKARLAAARVLILNG
                     KLDDALDQIQIAQLRRSQTRLEAEINRILRIAAIRPASEWERVIERRLDRGATRLAQM
                     AARDLADFVPGMNTALVKRALGDRKPLSPDARWVKDLLATLPQIQKTAGAILNRLARP
                     EQDTLAAADTLAQEWWAALVPPAKDRDAHAAGAMLALGVALMQYFAEASVDPTPIAGA
                     YRHIATEALHLVRRSRYQIEHNAIHTLLRIFEHLSASPEWLLDTWLLRVERALDLEAE
                     HGAYLDGITGGLPTVQRLLRGDERIGWELRFAHDLAAEPTMYEPAAALFERCSRAVES
                     GGTELAWSLAAATAKPPPAHLDVHWLAAMANPHVAAPWLAVAEGLMLSGRPREGFLAA
                     CKGMFAATAKERPAALEKLRGAWRASGITTSIDGDQAFEAGLAAASDDKLEIAVQHLR
                     WASACEPGNPKRAQSLAVALGRMGNAAEAIRVLAAHERNDAPRLIARVLVESGRYVDA
                     VPILRYASRRFRNAEDWALLATTAAKADNDAVAVDAGRWAMRLGSMDADVMMALATGL
                     YRLGEFVECEKIASQLITARTPQREIRIVGLHAMARALAGQGRHVDAHPYAKEAARLG
                     PNGDLAADLIETMDRIIAQQTPSVRTSTELSMERQAWADLEAGKFESLVNATTSPSWG
                     ITRAALAATEFRTLDESGIPVAPRALDTAIAILKRTVGTTNPDAVQARVRALRIRDNA
                     FIQIDAPPPLGMRFSWEEFERAYNERDKRPHRPSAILSYAR"
     gene            complement(18338..19552)
                     /locus_tag="VMZ53_05410"
     CDS             complement(18338..19552)
                     /locus_tag="VMZ53_05410"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS27920.1"
                     /translation="MKQLVVAFALLAACRGDNKDAGPSHGSALSVSGSGSTAGSLPKL
                     PLTEDGKAMLRALDAGIEGTRERPAEHIGLLLQRAGIRGRVEDYTRALAAAGAFAKAS
                     PTDEAALRARVQALVAVHRFGDARAAIAELAKVIHPSFLPELEVGIDQATGNLDRALA
                     TREQIMKDAPSPQTITLYAMTLAEAGRFDEALALLPKGTAAIRLNTPDFVSWLLFQWG
                     RIYEQKGDTALARDFYEEAYRRLPGSLEAAEHLVGVLVATGDTERAKRIVATAVAENY
                     HPTLRALAVKLGVSKESAADVAAEWERYVAALPEAFADHAARFYLDAGANPKRALELA
                     RLNFANRPTFAARALVVEAALAAGDPAGACAEVDALVEAPTRAHRFIAWKALSACGRK
                     EEAQRLGAALGI"
     gene            complement(19549..20346)
                     /locus_tag="VMZ53_05415"
     CDS             complement(19549..20346)
                     /locus_tag="VMZ53_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27921.1"
                     /translation="MKTTTTLLALLIALAACGDNKGRPDARQPPDGPPADAQCSNCPA
                     APALGTQIDRMGRPAINTLLNHGFDPGTGAGPAKNAYNADTMPSTWPATYLAEFKKNL
                     GVLDALDTGFCGNGRCELGEFGTAGATGVCAADCATATQVGTGDGCGNQVLYNGGMGT
                     TPALTSYDTLSSILAADELYVDTSKSTCAFYLAVEFGVATGLGNSTCGGRAPQYDVID
                     FSLSAIAVGINGFSTDGLFTPKMKDNAPAHTDYTSTFPFLGEPNDLP"
     gene            complement(20357..21151)
                     /locus_tag="VMZ53_05420"
     CDS             complement(20357..21151)
                     /locus_tag="VMZ53_05420"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HUS27922.1"
                     /translation="MKKLAFLGGAAGVGIVAILGIHHGQAADHADAPALATQPMADLN
                     DVYTWMTTDGTKVNLALTVSPFDNLAEAANMTRHFGPTVTYAFHITSRPGFQMAGTES
                     KIICKFADDTHGECWVTDSANKVVDYVTGDLSGANGRISATGKFRVFAGRRSDPFFFN
                     LGGVRSAVAAAEGVCAGACPPSTVGATDASGCMKVSKGQGDALRGAIGTKPATDLTST
                     PCDDTANIDCFIDANVMAIVIQIDKDELLHDTDKLLSVWASTHTGS"
     gene            21362..21979
                     /locus_tag="VMZ53_05425"
     CDS             21362..21979
                     /locus_tag="VMZ53_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUS27923.1"
                     /translation="MGTKLYVGNLNYNTTEASLREAFGANGREVASVSVIMDRETGRS
                     RGFAFVEMATAEAAQQALQELDGQELDGRQLRINEARERESRGPGGGGGGYRPGPRPG
                     GGGYGGPPGGGGGGYGGPPGGGGGYRPPGGGGFGGPPGGGGGYRPPGGGGGFGRPAGG
                     PPAGGGDNRRRGKGGDRDRDKERGGGGPDADRKKRRGRGGDDDGW"
     gene            22016..22945
                     /locus_tag="VMZ53_05430"
     CDS             22016..22945
                     /locus_tag="VMZ53_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS27924.1"
                     /translation="MTVSLPAWPAWIESQLPQGARRSMIDVGDGQRMHVSEWGPSDGR
                     PVLLVHGNPTWSFLWRKVIASVRRREGGEKLRLVAPDLIGLGLSTKPGAAEHSLDRHA
                     GWLGRMIDEVIRGPLVLAAQDWGGPMSLCAMATRRDRLRGIVVGNTAVGPPREGFKAT
                     LFHRLSQMPVVSHLLFRELGFPIGMLHLSQGDRSSIRGDVARAYRWPLRRWSDRAAPL
                     ALARMVPDSLGHPSVPSLRVSDELFRTAEVPVSIVWGTKDPVLGRVINHLERMRPDAK
                     VVRNDGAGHFLQEEVPDLLADAIMDVSSRASWS"
     gene            22942..>23226
                     /locus_tag="VMZ53_05435"
     CDS             22942..>23226
                     /locus_tag="VMZ53_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27925.1"
                     /translation="MKTKFEVAADDEGLRLDQVIPKHIAGLSRRKTRAVIDLGGVFVD
                     RTRVRVAGRLVKRGQVIEVNVGGAIERANEAPLAPRIVHVDDHIIVIDKPA"
BASE COUNT         3656 a         7637 c         8057 g         3876 t
ORIGIN      
        1 agctacagcg gcagcgcggc ttgcggcgcg ggcgaggcgg ggcgaggcgg cgggtggtac
       61 gtcaggtcgt cgtcgacgtg agctcccggt gggagcgggc cgcgcatgac gagctcgccg
      121 ccttcgacgg acgcgacggg atgcccgtcg acgtagagga cggcgttgcc gacgatcgcg
      181 gggacgcgcg ggccggcgga cagcacaccg acgaggttga gcgggtccgt cgccgcaaca
      241 cgcgcgactt cagccgcggg accggggttg cgcacgccgc gcagctcgtc gagagcttcg
      301 ggcagcgcga actgttcgcc gacgaagccc gtgacgaagc ggccgccgcg aatttcgccg
      361 cgcgcttcga ggcggcgcaa tgcggtaagt agatcgcgcc acggcggcag gctggactcg
      421 cgcgcgacga ggtcgcggaa tacgacgccg tagcgatgga ggagctggcg cgcagaggct
      481 tcggcgtcga gctgctcggg ctttgcatcg gcgagcaacg accagcggcc cgcggcggtg
      541 ggcagcgcgc gaagtgcgtg cgccggccgc tcgcgatcgc ggttgcgcgc cttcttgatc
      601 gcttcttgcc acttgcgcac gggcgtcgcc gcggcggcag cggcggctgc ggcggacgcg
      661 gccgcacgat cgaagtggct cttcacctca ccgcgcttgc gatcgacgag gacgcgcaag
      721 ctcgcaaacc catcggccgt cgcgaggccc gcgccaacga gctcccacaa cgcgtcttcg
      781 accgcatccg gcgagatgtc catgcccccg acgagatccg gcaagaacga tgcgccgacg
      841 ccgacgaggt gatcgcgcac gcgtgcagcg gtgggagaca gcacaggcgg ctcggcattc
      901 gcgacggcga cagcggcgcg taaccacgta gcgtcgcgac gaagcatgag cgcgagtggc
      961 gcggagcgcg acggcgcgtt gcgccgcgtc ggcgcctcgg gaaaggccgt ctcgagcgcg
     1021 acgcgtgcga tcgacagcgc ctgcagcacg gggaacttgc gtgcgagcat cccggcaatg
     1081 ccggcagccg cctgctgccg cgtcgcgcca gcgtcgagcg ctgctgcgag tgtcgcttcg
     1141 gccgcggcct gaatgacggc agcaggcacg ccatcgctca cggctggcgc aatccactcg
     1201 ggcaccacgc gctcacggtg agctcgagca tcccgcgcgc cgcctgcgtc accgccatcg
     1261 acggtgcggg cgtcacgatc gaaggcgcgg gcatcacgat cgccgttgcg ggcatcgcgg
     1321 tcatcacgat cgccgttgcg ggcatcgcgg tcatcgcgat cgccggtgcg ggcatcacga
     1381 tcgaaggcgc ggccatcgcg ggcatcgcga tcgccggggc gggcatcgcg atcgatggcg
     1441 cgggcatcga ggtcatcgcg gtcgaaggga tcgcgaccga tggtgctggc ggcgcgtgca
     1501 tcgccatcga atagatcgcc gtcgtgagcg gcggtggatg acttgcgagc acttgcgggg
     1561 gcgtggtcga gagcgctccg ctgttcgcgc gcgcgcgcga cccacgtggg ttcgccccac
     1621 agcttgcgat ttgcgttggc gggctttcgc gacttggctt gttgggcgac agcgcgcgtc
     1681 gcggcgcgtt ctccgatttg catcggctcg cgcaggatcg gcgggaatgc gtcgagctcg
     1741 gagcggtgcg acgcgacttg cgtcagcgcg gcgagcagct ttgtggcttc gtcggtggtt
     1801 aggggctcgg cttcggatgc gagcgtgacg cgcggcgaca ggcgacacca cacgaggtgg
     1861 ccggcgaggc acatctggtc gagccatgac gggtcgtagc cgtagatgcg cgacggcagg
     1921 atttcgcgct cccatgcgcc ggcggcggtc tcgaagcctt gcagctgctc gacgacgcgc
     1981 gcgaggccgt cgaggccgat cagctgcgtg ttttttgcga cgcgttgcca gcgcaggagg
     2041 aagcgcatca gcgcggcggc gctgaccggc tcgatctcct tgcggaggcg ggcgagggtg
     2101 aggcggtgga tccgcgcgag cacgcggcgg ttgcaccact cggtgtcggc taggagatcg
     2161 ctgtcgtcgg ctgcggtgag tgcttcgcgg actgtcttgc gtgcggccgt ggaggcggac
     2221 gtgaacgtgc cgcgcaaaat cgcgccgtca ccttcgagcg cgaggagcgc gccgaggatc
     2281 tcggggaggg gcaggccgag ctcgtcggcg agcgcgcgcg tgttgacggg accgcggtga
     2341 tcgaggtgcg cgctgacgag cttgttgagt gcgacctcgc gctccggtgt ttgtgcccac
     2401 gtgggcgtcg cgatcatcgg cgtgcattcg acgtcggcgc cgaggagtgc gcgggctgcg
     2461 ccgagccgtt cggtcgcgca ccacgcgacg cacgtgcgat cggcggcggc ggcggcggtc
     2521 gcaggtgcct gccaacgcag gcgacatgcg cggcctgtat cggcgagtgc ggcgaaccag
     2581 tcgtcgacgg ttccggcgct gtcgccccac gtgcggccga gatgttcggg cacgagccag
     2641 agcgcgagga gcgcgtcgtg cagctcgtcc gcgtcgcgga cggtgggcgc gacttcgtgg
     2701 gaggcgagct cgatcgcggc ttcgtcgagc gcgcccacgt tgtcgacgcc gtcggtggtg
     2761 atggaagcgg gcaggccgcg gcggagctgc acggcgcgcg tgcggcgttc ttcgagcggt
     2821 gcgtcgtcga ggaatgcgta tgggtttgcg ttgatcagct cgtgcgagag cggtgagggc
     2881 tcgacggtgt cgcgcgccat cacgcgaatc tcgccgcgct ctagctgctc gagcagggtg
     2941 cggaagccct cgaggtccat cgcctcgacg aggcagtcgc ggatggtctc gaacacgagc
     3001 gggtggtcgg gaatctcgcg cgggccgacg atgttttcgg cgcaggcctg cgcttgcggg
     3061 aacacgacgg agagcgtgtc ggcggcgcgc atcttgatga ggtgcggcgg gattttcttg
     3121 ccggcacgac gccgcagcac cgtgagcgag cgcgtgacgt tccagcgcca gcggatctcg
     3181 aacatcggcc gatcgagtag ggcttggatc agcgtctcgc gcgcttgatg cgacggcacg
     3241 tagccgaaga ccgtgtcgag cgggaagctg tgcggttggc cgagcgagat cacgatgccg
     3301 tcgtcggtcg cgatcgcttg gagctcgaaa tcgaacgtga cgcagaactt cttgcgcagc
     3361 gcgaggcccc acgcgcgatt gatgcggcca ccgagcggcg cgtgcaggac gagctgcatg
     3421 ccgccggcct cgtcgaagaa gcgttcggcg acgatcaagt ctttgcgggg cagggcaccg
     3481 agcgcagcgc gggccgcggc gagatatgcg actgcttgct gtgcggcgcg cagcggcatc
     3541 gagcaggact cggccagcca tgcggcgagc gcgtccatgc tctcggtggc gagacgctga
     3601 tcgatctcgc cgcgcagcgc gctgacttcg tcagacagct cgcgtgtgcg agcgggagcc
     3661 tcgccgaacc agaatggaat cgttggcggc tggccgcgtg cgtcctcgac gatcacgcgt
     3721 cccgcttcga tgcggcggat cttccacgac gtgttgccga gctggaagat gtcgccggcg
     3781 gacgagtcga tcgcgaaatc ttcgtcgacg gtgccgacct gcgtctcttc gggccactgc
     3841 acgacggtgt agttgttgtt gtcgggaatc gcgccgccgc tcgtgatcgc ggcaagccgc
     3901 gcggatcgcc gagcgcgcac catgcccgcg acgcgatcgc gatgcagata cgcgcccgtc
     3961 cggccgcggc gatcggacac gccctccgag agcatcacca tcagctgctc gagacgcgcg
     4021 tcttcgatcg cagcgtacgg cgtggctcca cgcacgagcg ccatcagctc ggcttcttcg
     4081 atgtcctccg ccgcgcacgt cgcgacgatc tgctgcgcga ggatgtcgag cggagcgtcg
     4141 cgcagctcga tctgatcgag gttgccggag cgcacaccgc gaatcagcgc cgcgcattcg
     4201 agcagctgat cgcgcgtgag cgcgaacatg cggcccttcg gcgtgccgcc gagcgtgtga
     4261 cccgagcgac cgacgcgttg gagcagcgtc gcgatcgagc gcggcgagcc aagttggacg
     4321 acgagatcca cggcgccgac atcgatgccg agctcgagtg atgccgtcgc caccgcgcac
     4381 ttcacctggc cgtacttcag cttctgctcg gcgttgtgcc gcagctcgcg cgacatcgat
     4441 ccgtgatgcg ctacgacgtg ctcttcgccg aggcgttgct cgagcgcagc cgctgcacgc
     4501 tcgacgagcc gccgcgtgtt gacgaacacg atcgtcgagc gatgctgggt aacgagctcc
     4561 gcgatccgat cgtagacgcg atcgaattgc tcgttcgatg cgacggctcc gagctcgtca
     4621 tcggtgatct cgatcgcgag atcgatctcg cggcggtggc ccgcgtcgac gatgtgcggc
     4681 atcggccgat gcgtgccgac gagcagccgg ccgatgcgct cgatcggacg ctgcgttgcg
     4741 gacaggccca cgcgcacggg cgcgcgaccg gagacctgcg tgacgagccg atcgagacgc
     4801 tcgagcgaga gcgcgaggtg cgcaccgcgc ttgtcgccgg cgatcgcgtg aatctcgtcg
     4861 atgatgatcg tcttcagctg accgagcgcc gcgcgaccgc tctgcgtcgt gagcaggatg
     4921 aacagcgact ccggcgtcgt gacgaggatg tgcggcggct ttttcgccat cttcgcgcgc
     4981 tcgcccatcg gcgtatcgcc ggtgcgaact cccacgcgaa tgtccgggaa cgcggtgcct
     5041 tcgatcgcgg cgcgctcacg cagctcggcg agtggcgctt ccaagttgcg atggacgtcg
     5101 ttcgagagcg ctttgagcgg cgagatgtag aggatcgagg tgtgattcgg cagcggctga
     5161 gtcatgccgc ggcgaacgag ctcgtccagg cacgcgagga acgcggcgag cgtcttgccc
     5221 gagccggtcg gcgcggcgat gaggacgtct ttgcccgcgg agatgcgagg ccagccttcg
     5281 cgctgcggcg cggtcggccc gccaaagcga tcgcgaaacc aaccgccgac cagcgggtgg
     5341 aatgcgtcga gaacgtcggc ggtagcgacg gacaccgggc ccgtcagagt actaaagctc
     5401 gacgcagaac ggaacggatg tacagggtgg agatctctac cagtcgccgc cgggaccagg
     5461 cccaaacgag tcgcaagacc gttgacagtt tgtcttgttc gcgtgcgcgc agagctcgtt
     5521 ggtgctgccg agcatgttcg tcccaccgag gttgcgggcg taaatacgga cgccgtcgat
     5581 atcgcccgtg aagtcgggag cgatcttcgt cccattcgtc ccggtcttcg agatcatcgg
     5641 attcgcgtcc ctcgcgcagt cgcgactcga gccatcgacg aacacgcgga cctgcgagcc
     5701 gtcgaaggtg caggcgacat ggtgccattc gccgggcggt atgctcgtcg cgttgctgaa
     5761 ggagtgaacg tctttgcctg ccatctcgca gccgacaaag cccgcatcat tgagcgtcaa
     5821 acggtactgg ccctggttgc cgatgatcgt cgacgcgaga tagcgcgttg gccggatcca
     5881 cgcttcgatc gtgatcgcgt tggtgatgtc gagcgtcggt gtctccatga tcgaaaggct
     5941 cgcggaggca tccatcgtga tcgccgggtc cggcggcgtc gttgtacgca tcgtcgcctt
     6001 gaggccgacc gcctgcgcat cgagctgcat cgacgacccg tcgcggacga tcgggttgat
     6061 cacgccgtcg ttgaattcta agcagagctt cagctgctga tcgggatacg cgcaggttcg
     6121 cgccccggta ccgggcggct gaacgatggt gccaccggcg tcgggctgcg ggtcgacgga
     6181 gtcgtcgccg ttctcgagct gaccgtgctc gaagccgcag ccgctgccga tggccgcgac
     6241 ccacagcgtc gtcgagacga ggaacctcac gagcggctat gctgcaacca ccgcgcccac
     6301 gctaaccgct cgaacgcgca cgcgtcggac ggcccgcact gaagacgccc ccacccggct
     6361 gtccggccgc ggccccgtgg gtgaaatcac ccaatgcgca ggaagctgta gtgataggcg
     6421 atctgctcgt cggaatagcg gtgctcgcga ccgactgtgc aagcttcgcg cacctcgagc
     6481 cacgcacgcc aaacgtcggg ccccgtcgac gacatggctc gcgagaatgc atcgaggcag
     6541 tcgcgatcgc agctgcaggc gcgcggcgca gcgacgcgcg gcggaggttc gccatcgcac
     6601 gtgatcacgg cgcgtaacgc gaaccacggc ccgtacacgg gatggatcac gagctgcgtt
     6661 ggcgagagcg agccaatgcc cgctgcgacc gcgagacgtt ggaacggcaa gaacgcgccg
     6721 tcgtactggc ggtgaccgta atagacccgc gcgttcggca gcgacgtggc gacgcgagcg
     6781 atcacgtgat cgatcacgcg ctcggtgtag agctcgagtg gatgactcgc gcttgcgagc
     6841 tcggggtcat tcgcgcgcgc agcggagaac accggccaca gcgcacgggt attgccgacg
     6901 aacagcccga gccgaccacc gtcgagccgc gcgcccgtct cggcggcaca tgcgcgtgca
     6961 tcgaacggat gcaggagatc aaatcccgct gctgcgaggt ctgccgcgag cgccgccagc
     7021 acgtcgagat ggtggcatca tctgcgcgtg cctgcgaggc tgcaccacga attgatcggc
     7081 tccgcgccgc gccgcatgtt gctcacgcat ggcatctacg gcgcaggcag caactggcgc
     7141 ggcatcgcgc gaaagctcgt cgcagcgcgg cccgattggt ccgtcgtgct cgtcgacctg
     7201 cgcaaccacg gtcgatctga atccggcgag ccaccgcata cgctcgaagc atgcgcggag
     7261 gatctgcgcg cgctcgtcgc ggagctcggc tcgatcgagg tgctcgccgg ccacagcttc
     7321 ggcggcaagg tcgtgctgca agcgcgcccg ctcgtcggtg acgtcgcgca aatctggatg
     7381 ctcgactcga gcccgagcac gcggcgcgat ctcgcaaacg atccgacaag cgcgctcgcg
     7441 gtgctcgagc tcctcgaacg cctgccgcgc acgtggccat cgcgcgatgc gttcgtgcaa
     7501 gcgatcgtcg acgccggcca cacgaaggat ctcggcgcgt ggctcgggat gaacgtcgtg
     7561 gcggatggcg acgtgtacac gttacgtctc gctctgccgg cgctgcgcga gatgctcgcc
     7621 gattactgcg cgcgagacct gtggcacctc gtcgaggacg cgtcccttcc gggaaccgtc
     7681 gagatcgtga ttgccgaccg atcgagcacg ctctcggcgg cggaccgcga gcgtctgtcg
     7741 gctccgcttg cgcacatcca tgcgcatcac atcgacgcgg gacattggct ccacatcgac
     7801 gcacccaccg ccgtggtcca gctgttcgca gccgcgctgc caacaacgtt gtcctgagac
     7861 ttccgtaacg tcgcgacact tcacgtgacg gatctggccc ggttccggca ctaaccccta
     7921 tcgtggccgg agctggagtc gattgtcccc ggaccgcccg gactgcagcg agggactgcc
     7981 aggcaacgcg cgtgcattgg acgtgtcgcg cctccatgcg catcgccctc tgcctcgtca
     8041 tgctcctgac ggcgactgcc accgcggacg atggcgatca cacggagaag gttcgctggc
     8101 gcgcaccgaa gggctgcccc gacgcccgcg ggttgcgcca gcgcgtcgag aagcgactcg
     8161 agcgttcgct cgatgatgtc tccgtcggcg tcgacgtgga tgtcgagaag cgtgccggtc
     8221 gctacaccgc gacgatcgat cttcgcgcgg tcaccgttgc gaacgacgtc cgtcacctga
     8281 cgagtcgccg ctgcggagag ctcgccgatg cagttgccgt gatcgtcgcg cgcgtcgcga
     8341 gcgatgcact cgcgcaccag gatgcggcgc ggcctcccgg cgatgtcatc gttgtcacgc
     8401 ctgacgccgc agacgcaccg accgtggccg atgcattacc cgccgcgcgc aagacgaccg
     8461 cgttcgccga ggagccgacg gtgcccgagc tgcgccagca ggtcgattcg ctctccgacg
     8521 gacggtacgt gccgaagcga tgggcgatcg gcggccggct ctccacggtg agcgcgatcg
     8581 gcgtcctgcc gcggatcggt accggcgccg agctcgcgct gacgatccga cgtcaggatc
     8641 actttgccga gcttgcgggc acgcgatgga tcgagagctc tgcgcagatc tacacgggcc
     8701 gccctgcgaa gctcgatgtc ggcctcgacg tcgctgccgt tcgctttggc tggcggccgc
     8761 gaaacatgcc gctgcgcgcg tggctcggcg tcgagagcgg ctcgatgcgc ggcaccggcg
     8821 tctcgctgcc aacgccccag ttctcgtcgg aacgctggct cgctgcgggt gcgggattcg
     8881 gcatcgcgtg gcagatgcgc ccgtggattc gtctgttcgg cgcgaacgag acgatgctcg
     8941 cgatcgaacg cgcgcgcttc agcaacggcc aaggcatggt gctctacgcg ccgaacccga
     9001 tgtcgtttcg cacgatcgtc ggattggagc tcggatggca gtgaaattcg ctgacggatt
     9061 ggtcgcgctc gcgccaccaa cctccgagat gaccgcgctt gcccacgcga tcgacgcacc
     9121 cgccgtgccc agcttcgacg aggtttacac ggcgcacttt gcattcgtgt ggcgcgtgct
     9181 gcggacgctc ggagtgcccg agcgaggcct cgaagatgcg gcacaagacg tgttcgtcgt
     9241 cgtccatcgc aggctcgccg agttcgaagg ccgagcagcg atcacgacgt ggctgttcgc
     9301 gatcgcacgc cgcgtcgcct cctcgcatcg ccgcgcgacg gggcgtgatg ccagccgcac
     9361 ggagcctctc gccgacgagc ccgccggcgg cgccgacaca ttcgcggcgc tgtctcgcgc
     9421 acaggcctcc gccaccgtca tgggaattct cgaaacgatg gatgaagaca aacgcatcgt
     9481 gtttgcgctc gtcgagctcg agcagttgtc ggtgcccgaa gtcgcacgca cgctcgacat
     9541 caacctcaat accgcttact cgcgcctgcg cctcgctcgc caggcattcg aaaccgccgt
     9601 tcgcgcacgc gccggccgag gagtgatccc atgaccgagc tgtcgcgctc tctactcgca
     9661 gcggcccgcg acgggctcgc acccgacccc gatgttgccg ctcgcgtgcg tgcgcgcgtt
     9721 gctgctgccg tcggtgcgcc gctcgccaag cccgtcaccg caaagccggt cccgagtggc
     9781 ggctccggcg cgttactgaa gctcggcgcg ctggtcgtcg tcgcgggtgt cgtcactgga
     9841 ggcctcgcgc ttcgccgtga cgcgcgcatc gaatcgccgc gtgtcagcgc gtcgacgacc
     9901 gacgtcgacc tcgagcccgc ccagtccgcc tcgacgcagg agcacgccgc tccgcccgcc
     9961 gctgcaaagc ccgcgcgcag cgtggcgccc gcatcgctcg cacgcgaggt cgagctgatc
    10021 gatcaagcga tgtcggcact gcgacgcgag gagccggccg ctgcgctcgc attcatgcgc
    10081 acgttcgatc gcgagacgct cggccatgca cagatggcag aagaagccgc cgcgatcacg
    10141 atcgaagctc ggtgcggcct cggcgaagat gtcgccgctc gtatcgcagc gttcgacgag
    10201 gcgtggccga gctccgccga gcgcagccgc atcaccgacg cgtgccgcta gcgtcactcg
    10261 tcgtcgggcg tcgtctcggg ctcgggcttc gagccggtcg gcagcttgcg gggcgggaga
    10321 agctcgggct ccgtgacgac ctcgcacgcc atgaagatcg cggcatcgag cttcttgaac
    10381 tcgcgtttgc gataccagcc gagcgcggtc tcgttgtttt cggcgacctc gagcacgagg
    10441 tgatcgaggc cgtggacgcg cgcgaggtga tgcagctgct cgagcacgaa tgagccaacg
    10501 cccttgccat gccaatcggg atgcaccgcg agctcgtcga cgaacagcgg cttcatgtca
    10561 cgcttctcga agtagcgcgg gttgatccag ttgtcggatc cagtcacttc gtagctcgct
    10621 tcggcatacg cgacgatctc gtcgtcgacc tcgaacagga gctgctcggt gccttcctcg
    10681 tcgaacactt cttcgaagcg gcggcgcgag cgcggccgtt gatactcgac tgtctccgcg
    10741 ttcacctcgc gaaacgagag cttcaagaac tcccagaccg cgccgatgtc gcgcttcatc
    10801 atgtggcgca cattgatcgt cggaccggcg gggcccttct tggctgccgt cttgcccgcg
    10861 gtcttggtgc ggcgctgctt cgccatccgc ggcagggtat tacgagccga cgcgcgctac
    10921 cagttgctgg acctgctcga cgaactgcga cttgtgatgc cgctcgaatt cggtccaggt
    10981 cgtgaacgtg ctcggatcgg cgcggtcgac gaagatcttg ccgtgcaggt gatcgacctc
    11041 gtgctggtac gtgcccgcct tgagcccgtg gacgacctcg tcgatcgcgt tgccgttgcg
    11101 gtcccacgcc tgcacgcgga cgtgcacgtt gcgcttcacc ttgccgcgca ggttcgggac
    11161 gctgaggcag ccctcgtagt tatcgaacag ctcgtcgtcg accggcgtga gcacgggatt
    11221 gacgagcacg gtcagcggaa tctgcggctt gtacggatag cgcgggttat tgcgaacctc
    11281 gatcgcgcag atgcgaacgg gctcgtacac ctgaatcgcg gcgagccccg cgccatcggc
    11341 atcgcgcatc gtctcgatca gatcgtcgat gaactgctgc atcgccggcg acgccagctc
    11401 ctcgcgcgtc acctcgcgcg ctgtctgccg aagcaccggg tgaccgatct gcgcaatctt
    11461 gcgaatcgcc atagccccac cactctagcg catcgcggcc gacgcgacgg cacccggatt
    11521 ttccgcacca tcggtcccaa ccgcttgttc gatcgtcgca aacccagccg ctttcaccat
    11581 cgccgctagc tcgcgctgta cgcgccgtgg ccacagcggg ccttcataga tgaagccggt
    11641 gtacgcctgc acgagcgtcg cgcccgcgcg aatgcgtccc cacgcgtcgt cggcgctctc
    11701 gatcccgccg acggcaacga gcacgagccg cgtgccgacc ctcgcgcgca gccggcgcaa
    11761 tacgtcgagg cttcgcgtct tcaacggcgc acccgacaag ccgcccgcac cgatcttcgt
    11821 aacggccgcc tcgctcgtcg ccagccccgt gcgcccgatc gtcgtgttcg tcgcgatgat
    11881 cccgtcgagc ccgagctcga gcgcgaggtc agcgatcgca tcgatgtcgg catccgccaa
    11941 gtcaggcgcg atcttcacga gcagcggaac gcgccgcatc ggcgacgcca tatcgcatgc
    12001 cgctctcacc gcgacgagca acggccgcag cttgtcgacg gcctgcaggt cgcgaagccc
    12061 gggcgtattc ggcgagctga cattcacgac gaggtattcg gcgagcggcg ccagcgtctt
    12121 cgcgcttcgc gcaaaatcgt cgagggcctg ctcctcgctc acgcgcttcg tcttaccgat
    12181 gttcacgccg acgacgccgt gccgcgtagg cacagacagc cgcttcgccg ccgcgtcggc
    12241 gccgtcattg ttgaagccca tgcgattgat gagcgcgcga tccttcgtga gccgaaacat
    12301 ccgcggcttg ggattccccg gctgcgcctc cgccgtgacg gtcccgatct cgacgaaccc
    12361 aaagccgagc gccaacagcg cgtcgacgcc cttcgcgtct ttgtcgaacc ccgcggcgag
    12421 cccgagcggt cccgcgaatt cacgcccgaa cacgttcacg cgcaacgctg catcacgcgg
    12481 cccgagcacg cgctgcatca gagcacgcat tcctggcacc gcgacgaacg cgcgcaacag
    12541 cgcgaacgac acgcgatgcg ttccctcgac gggcaatcgc gagagcacga gccagtagag
    12601 gaggcgatac atctgcgcgg accttagcat cgccgaaacg caaaacgccg acccgaaggc
    12661 cggcgcattg cgattcaaga gaccgaggac tactgcagct gctgcagttt gcgcgcggcc
    12721 ttgaggccct tgtagcgctc gattgctgcc tcgagcatcg gatccgagcc ctcgacggga
    12781 cggttgaccg tcggcgtcgg agcaacttcg ctctgctgga ggaagaacgt caggccgccg
    12841 atcgacgtcg agcatccgcc cggcatcagc tcgcccgtct cctgatcgat ctccttgaac
    12901 ggaaccatcg tgacggtgcc gttgatcttc gagtccttcg ccgtcggggc gccggcgccc
    12961 gtgaacgccg tgaacgtcag agcgccctgc tgcgcccagt agaggttctg gatcgtctgc
    13021 gagccgtcga agtcaccgaa caccatggcg tcggcaacga tcgagccggt cgcggtcgcg
    13081 tcggtctcga acgcgtacgg cgtgttggtc atgaagccgg cggtcggctt gatgacgtcg
    13141 aggatgagtg cgtcgatcgg cggcgtggtg cccatcgaga ggcgcgctgc gataaagaac
    13201 tcgagcttgc ccatgtcggc gcccgtcgtc gggacgtcga agttctggcg aacgacgggc
    13261 atcgccatcg agccgaacgt gagggtgccg agcgagtcca tcacaccgca gaggtgcggg
    13321 gcgtcgggcg ctgcatccat gaacttcttc gcatcgggaa cgacgggctt gccatcgtcg
    13381 ccaccgcaac caacgagcgc ggcaaccgca ataagagaag aaccaaacat ctgaatacgc
    13441 ataaagcctc ctgagattcg caaacgattc gctgcggcgc cttggccggg gtgattgagg
    13501 acggacctta tttagatttc ggccgggagg gaatgagcga ctgctgtcaa attgctgcgt
    13561 cgagtccgct atgtttcggt agcgcttgtg acgaacttcg ccttcgcgac ctgcttagcc
    13621 ggcctggagc ccgccctgaa actcgacgtc ggccgggtca gaccgggctt gcgcttcgct
    13681 tattcaaggc ctgggttggt gacgttcaag agcgatgtgg cagttcgccc cgatgacccg
    13741 ccaggctccg tgttcgcgcg ggtttggggg cggtcgattg gcccggccac tgtcgaggat
    13801 gtggcccgcc agctggccga tttgggcacg actcgtgttc acgtttttgt gactgcacac
    13861 gatgagcagc acagggggcg ggacacccgc gacaccgcgg aagaggaggc gctcagaccg
    13921 tggcgcaccg cactcggtga gatgccgagc ggacccgcgg aggagggcga tctcgtcggc
    13981 aatgtgatcg ttgccgcagg cgaggagaag gcgtggctcg gcgtgcatcg gcactcggac
    14041 gcgacgcacg tgaagtggcc cggcggtgcg atccccgtcg aggttcctgt cgatgcgcca
    14101 tcgcgcgcgt tcgcaaagat cgaagaagcg atcgcgtggg cgaagctccc gatccaagcg
    14161 gggcacgtcg cgctcgagat cggtgcggca cccggtggtg cggtgatggc gctcgcgcga
    14221 cgcggtgtcg aggtgtgggg tgtcgacacg ggagagctcg cgccgaacgt gaaggcgatg
    14281 ccgggcgtgc atcacctcgc gatgaaggtc ggtgcgctgc ggtgggagca gctgccgccg
    14341 cgcgtcgatt ggctactcgt cgacgtgaac ctcgcgccgc aggtcgcggt gcacgaagtc
    14401 gcgcgcctca tgccgaggct cagcaagacg ctgcgcgggt gcgtgttcac gttgaagctc
    14461 aacgagctgt cgttcgttcc cgagctgccc gtgttggccg agcggatcgc ggcgttcgga
    14521 tttaccgacg tgcgcatgcg gcacctgccg tcgaaccgtc gcgaagtctg ctgcgtcgcg
    14581 ctgcgctagc gcgcgtacga gaggatcgcg cttggccgat gcgggcgctt gtctcgctcg
    14641 ttgtatgcgc gctcgaattc ttcccaggag aagcgcatgc cgagcggcgg cggtgcgtcg
    14701 atctggatga atgcgttgtc gcgaatgcgc agcgcgcgca cgcgggcttg gaccgcgtcg
    14761 ggattcgtcg tgccgaccgt gcgcttgagg atcgcgatag ccgtatcgag tgcgcgaggg
    14821 gcaaccggaa tgcccgactc gtctagcgtg cggaactcgg tcgcagcgag cgcagcgcgc
    14881 gtgatgcccc acgacggtga ggtcgtcgcg ttgacgagcg actcgaactt gcccgcttcg
    14941 agatccgccc acgcttggcg ctccatcgac agctccgtcg acgtgcggac tgacggcgtc
    15001 tgttgcgcga tgatgcgatc catcgtctcg atcagatcgg ccgcgaggtc gccattgggt
    15061 ccgagccgtg ccgcttcctt cgcgtaggga tgggcgtcga cgtggcgacc ttgcccggca
    15121 agcgcgcgcg ccatcgcgtg gagaccgacg atgcgaatct cgcgctgcgg cgtccgcgcg
    15181 gtgatgagct gcgacgcgat cttctcgcac tcgacgaact cgccgaggcg atagagcccc
    15241 gtcgcgagcg ccatcatgac atcggcgtcc atcgagccga ggcgcatcgc ccagcgaccc
    15301 gcgtcgacgg caactgcgtc gttgtcggct ttcgcggcgg tggtcgcgag gagcgcccag
    15361 tcttcggcgt tgcggaaccg gcgcgaggcg tagcgcaaga tgggcaccgc atcgacgtag
    15421 cggccagact cgacgaggac gcgagcgatc aggcgcggcg catcgttgcg ctcgtgcgcg
    15481 gcgagcacgc gaatcgcttc ggcggcgttg cccatacggc cgagcgcgac ggcgagtgac
    15541 tgcgcgcgct ttgggttgcc gggctcgcac gccgatgccc agcgcagatg ctgcaccgcg
    15601 atctcgagct tgtcgtcgga cgctgcggcg aggcccgcct cgaacgcttg gtcgccgtcg
    15661 atggaggtcg tgatgcccga cgcgcgccac gcacctctga gcttctcgag cgcagcgggc
    15721 cgctcttttg cggtcgccgc gaacatgcct ttgcacgcag cgaggaagcc ttcgcgcggc
    15781 cgtcccgaca gcatgaggcc ctcggccacc gcgagccacg gcgccgctac gtggggattg
    15841 gccatggcgg cgagccagtg cacgtcgaga tgtgcgggcg gcggcttcgc cgttgcggcg
    15901 gcgagagacc acgcgagctc ggtgcctccc gactcgacgg cgcgcgagca ccgctcgaac
    15961 agtgcggccg cgggctcgta catcgttggc tcggcggcga gatcgtgcgc gaagcgcagc
    16021 tcccagccga tgcgctcgtc gccacgcagc aggcgctgca ccgtcggcaa gccgcccgtg
    16081 atgccgtcga ggtacgcgcc atgctcggct tcgagatcga gcgcgcgctc gacgcgcaac
    16141 agccaggtgt cgagcagcca ctcgggcgat gcgctgagat gctcgaagat gcgaagcagc
    16201 gtgtggatcg cgttgtgctc gatctgatag cgcgagcgac gcacgaggtg cagtgcctcg
    16261 gtcgcgatgt ggcgatacgc gccggcgatc ggcgtgggat cgaccgacgc ttcggcgaag
    16321 tactgcatca gcgcgacgcc gagcgcgagc attgcgccag cagcatgggc atcgcgatcc
    16381 tttgcgggcg gcacgagcgc tgcccaccat tcttgcgcga gcgtgtcggc ggcagcgagc
    16441 gtgtcctgct cgggacgtgc gaggcggttg aggatcgcgc cggcggtctt ctggatctgc
    16501 ggcagcgtcg cgaggagatc cttcacccag cgcgcgtcgg gcgagagcgg cttccggtca
    16561 ccgagggcgc gcttcacgag cgccgtattc atgccgggca cgaagtccgc gaggtcgcgc
    16621 gccgccatct gcgcgagccg cgtcgctccg cgatcgagac gccgctcgat cacgcgctcc
    16681 cattcgctcg caggtcgaat cgcggcgatg cgcaggatgc gattgatctc cgcttcgagc
    16741 cgcgtctggc tccgacgcag ctgcgcgatc tggatctgat cgagcgcgtc gtcgagcttg
    16801 ccgttcaaga tcagcacgcg cgcggcggcg aggcgcgctt tgccgtcggc gaggaggcca
    16861 ttgccgtcga ggccggagca atgatgaaac gcgatctccg cctggtcctc gcgaccgagc
    16921 gtgttgagcg actcgatcat gatcgccgag tcttcgtcgt cggtgatccg cccgcgcgtg
    16981 agcccgagga ccgcgcgacc cggatcgccg cggtggtagc cctcccacgc gtatgcgcgc
    17041 gcgagcagac cggcatcggt cttccagtgc gagatccgtt tgtgtgccca cgcgaacgcg
    17101 tctttcgtcg cgggatcatc cggcagttgc gacagcgcgc cgtctgcgag ctcgatcgct
    17161 tcttggatgc ggccgacgtc gaggtgcgcg aatgcgatca agagcgctgc gtgatgcggc
    17221 gcttgccagt cgttcgccga gagcttgccg ctctgcgcgc ggtcgagaat ctcgcgacac
    17281 cgatactgcg cttctgcgaa cgcctggccc gggcgtccga cttgccgcag cctgcgcacg
    17341 caatcgagat gcgcggcacc aaaatccggc gccgcgacgg tcgcgcggtc cgcccagcgg
    17401 tagctctctt cacgccgcgc ttgctcggcg agctgcagcg agtagttgtg acacgcgtac
    17461 ggattccccg gcatcagcga ggcggcaatg ccttgatgcc gcacgcccat gttctcgatc
    17521 ggccgatcct gatcgaacgg ctcgccccag atcgacagat gcggcttgcc gtacatcggg
    17581 cactcgctga cgagcggcaa gatgtcgcgt cgatccgcag cgccgcgcat gaggcccgag
    17641 acgtacgcgt tgcgccgcgc cacgggatgc gtgggatcga gctcgagcac ttttggatcg
    17701 agcgtgcgcg cagcggcgcc cgacacttcg gcgagcacgc gcagatacgg aaccgtcatc
    17761 ttcggcagcg gctcgaggag ctttggcgga atcgtcgtgt agtcgttgcg cagcgacagc
    17821 atcgccgccg ctttcaccag gcgatcgccg gcggcgcgaa tgtcttcgtg cgtgaactcc
    17881 ggcagcgagg cgaggagcgg cagcctgcgc gccgcgagcc actgcgtcag gcactgtgaa
    17941 atctgctgcg acagcgagag gtcagggctc gacgtccact cgtcgttgag gccgttcggt
    18001 tggcgcgacc ggagcagtgt tgcctgcggc cgcccacgat cgacgctgat ctcgaaccac
    18061 agcacttcgt gccgccgcgc gatccgaaac agccaatcga tcgtgtcctc gatctgcggg
    18121 tgattcgcgt cgaggaggcg gcccgcttcg tcgccgaatc gctcgtcgtc atcaccgagc
    18181 gtgacaacgg gatgccgcaa gaagtggtcg aacaccacga gcgcgagcat gcgcgccacg
    18241 tacggcgtcg acgccgactc ggccagcggc accacgagcg tgctggcgag aaacgtcgcg
    18301 cgatgcggat acggcagcgg caagcgagcc gagaatacta gattcccaac gctgccccga
    18361 gtcgctgcgc ttcttcctta cgcccgcacg ccgacagcgc tttccacgca atgaagcggt
    18421 gcgcgcgggt gggcgcctcg acgagggcat cgacttccgc gcacgcgccc gctgggtcac
    18481 ccgcggccaa tgctgcctcg acgacgagcg cccgcgctgc gaacgtcggt cgattcgcaa
    18541 aattcaatcg ggccagctcg agcgcgcgtt tggggttcgc gccggcatcg agatagaagc
    18601 gcgccgcgtg gtcggcaaac gcctcgggca acgcggccac gtagcgctcc cattccgcgg
    18661 cgacatcggc tgcgctctcc ttggacaccc ccagcttcac ggccagtgcg cgcagcgtcg
    18721 gatgataatt ttctgcgacg gccgtcgcga cgatgcgctt ggcgcgctcg gtatcgcccg
    18781 tcgcgacgag aacaccgacg agatgctctg ccgcctcgag ggagccaggc aggcggcgat
    18841 acgcctcttc gtagaagtcg cgcgcgagcg cggtgtcgcc cttctgctcg tagatacgtc
    18901 cccactgaaa cagcagccag ctcacgaagt ccggcgtgtt taggcgaatc gccgcggtcc
    18961 ccttcggcag cagcgcgagc gcctcgtcga agcggcccgc ttccgcgagc gtcatcgcgt
    19021 agagcgtgat cgtctgcggg ctcggcgcgt ctttcatgat ctgctcgcgc gtcgcgagtg
    19081 cgcggtcgag gttgccggtt gcttgatcga ttcccacctc gagctcgggc agaaagcttg
    19141 gatggatcac cttcgcgagc tctgcgatcg cagcgcgcgc atcgccaaat cgatgcacgg
    19201 cgacgagcgc ctgcacgcgt gcgcgcagcg cggcctcgtc ggtcgggctc gctttcgcga
    19261 atgcgccggc ggccgcgagc gcacgcgtgt agtcctcgac gcgaccacgg atccccgctc
    19321 gctgcagcag tagaccgatg tgctcggcgg gacgctcgcg cgttccctcg atgccggcat
    19381 cgagcgcacg cagcatcgcc ttgccgtcct cggtgagcgg cagcttcggc aggctgccgg
    19441 cagtcgagcc ggagcccgaa acggacaacg ccgacccgtg tgaggggccg gcgtctttgt
    19501 tgtcgccgcg gcacgctgcg agcagcgcaa acgcgacgac cagttgcttc acggcaagtc
    19561 gtttggttcg ccgaggaacg ggaacgtcga cgtgtagtcc gtgtgagcgg gcgcgttgtc
    19621 cttcatcttc ggcgtgaaca gaccatcggt cgagaagccg ttgatgccaa cggcgatcgc
    19681 gctgaggctg aagtcgatca cgtcgtactg aggcgcacgg ccaccgcacg tcgagttgcc
    19741 gagacccgtc gcaacgccga actcgaccgc aaggtagaac gcgcaggtcg acttgctcgt
    19801 gtcgacgtac agctcgtcgg cggcgaggat cgaagagagc gtgtcgtacg aggtcagcgc
    19861 aggcgtcgtg cccatgccgc cgttgtagag gacctggttg ccgcaaccgt ctcctgtgcc
    19921 gacctgagtc gccgtcgcgc agtcagccgc gcaaacaccg gtcgcaccgg cggttccgaa
    19981 ctcgccgagc tcgcagcggc cgttgccgca gaagccggta tcgagtgcgt cgagcacgcc
    20041 gaggttcttc ttgaactcgg cgaggtacgt cgcgggccag gtcgacggca tcgtgtcggc
    20101 gttgtatgcg ttcttcgccg gtcctgcgcc tgtgcccgga tcgaagccgt gattcaacag
    20161 cgtgttgatc gcgggacgac ccatgcgatc gatctgcgtg ccgagcgcgg gcgcggcggg
    20221 gcagttcgag cactgagcat cggcgggcgg accgtcgggc ggctggcgcg cgtccggacg
    20281 acctttgtta tcgccgcagg cggcgagtgc aatgaggagg gcgagtagcg tggtcgtggt
    20341 tttcatggtg atcacctcac gaccccgtat gggtgctggc ccacacgctc aagagtttgt
    20401 cggtgtcgtg gaggagctcg tccttgtcga tctggatgac gatcgccatg acgttcgcgt
    20461 cgatgaagca gtcgatattg gccgtgtcgt cgcacggcgt gctggtgagg tccgtcgccg
    20521 gtttcgtgcc aatggcgccg cggagggcgt cgccctggcc cttcgagacc ttcatgcagc
    20581 ccgatgcgtc ggtcgcgccg acggtcgatg gcggacaggc accagcacag acgccttctg
    20641 cagcggcgac tgcggagcga acgccgccga ggttgaagaa gaacgggtcc gagcgacgtc
    20701 ccgcgaacac gcggaacttg cccgtcgcgc tgatgcgacc gttcgcaccg ctgagatcac
    20761 cggtcacgta gtcgacgact ttgttcgccg aatccgtcac ccaacattca ccgtgcgtat
    20821 cgtcggcgaa cttgcagatg atcttgctct cggtccccgc catctggaaa cctggacggc
    20881 tcgtgatgtg gaacgcgtac gtcaccgtgg gaccgaagtg ccgcgtcatg ttcgcggcct
    20941 ccgcgaggtt gtcgaacggc gacaccgtca acgcgaggtt gaccttggtg ccatccgtgg
    21001 tcatccacgt atagacgtca ttgagatcgg ccatgggttg cgtggcgagc gctggcgcgt
    21061 ctgcgtgatc ggcagcctgg ccatggtgga tgccgaggat ggcgacgatg ccaactcctg
    21121 cggctcctcc gaggaaagcg agtttcttca agcgggtccc ccttgtgaag gtgcgaccta
    21181 ctctatgcct gaaactgtcg cagggggcaa ttccggcacg gtttcggata cctcacctgg
    21241 tgatctagtc acacatcgcc tgtgatacgc tcgatcctga gcgtggggtg gacactaatg
    21301 atccatctgt cgctcgagcc agaacctctc gcagtccgct gaatcgccag gagacacgtc
    21361 cgtgggaacg aagctctacg tgggcaatct gaactacaac acgaccgagg catccctaag
    21421 agaagcattc ggcgcaaacg gccgcgaagt agcaagcgtg tcggtgatca tggatcgcga
    21481 gacaggacgc tcgcgcggct ttgccttcgt cgaaatggcg acagcagaag cggcgcaaca
    21541 agcgttgcaa gagctcgatg gccaggagct cgacggtcgc cagctgagga tcaatgaagc
    21601 tcgcgagcgc gagtctcgtg gtcccggtgg tggcggcggt ggttatcgcc cgggcccgcg
    21661 tccgggtggt ggcggctacg gcggtcctcc cggcggtggc ggcggcggct atggcggtcc
    21721 tcctggcggc ggtggtggct atcgtcctcc cggtggtggc ggcttcggcg gtcctcccgg
    21781 cggcggtggt ggctatcgtc ctcccggtgg tggcggcggc ttcggtcggc cggccggtgg
    21841 tcctcccgct ggcggcggcg acaaccgtcg tcgcggcaag ggcggcgatc gcgatcgcga
    21901 caaagagcgc ggtggcggcg gacccgacgc agatcgcaag aagcgtcgcg ggcgcggcgg
    21961 cgacgacgac ggctggtaga gagcagtcct tccgcgcgtc gagtatgttc cgcgcgtgac
    22021 ggtgtcgtta cccgcgtggc ccgcatggat cgagtcgcag ctgccgcaag gcgcgcggcg
    22081 ttcgatgatc gatgtcggcg atggtcagcg catgcacgtg agcgagtggg gcccgagcga
    22141 cggacgcccg gtgctcctcg tgcatggcaa cccgacgtgg agctttctgt ggcgcaaggt
    22201 catcgcgtcg gttcgccggc gtgagggcgg cgagaagctg cggctcgtcg cgcccgatct
    22261 gatcggcctc gggctctcga cgaagccggg cgcagcggag cattcgctcg atcgtcacgc
    22321 cggttggctc ggccgcatga tcgatgaggt cattcgcggc ccgctcgtgc tcgcggcgca
    22381 agactgggga ggcccgatga gcctctgcgc gatggcgact cgtcgcgatc ggctgcgcgg
    22441 catcgtcgtc ggcaacacgg ctgtcggccc accgcgcgaa ggcttcaaag cgacgctgtt
    22501 ccatcggctc tcgcagatgc cggttgtgag tcatctcctg tttcgcgagc tcggctttcc
    22561 gatcggcatg ctgcatctct cgcagggcga tcgctcgagc attcgcggcg acgttgcgcg
    22621 cgcatatcgc tggccacttc gccggtggtc cgatcgcgcg gcgccgctcg cgctcgctcg
    22681 catggtgccc gactcgctag gtcatccttc ggtgccttcg ctgcgcgtat cggacgagct
    22741 ctttcgcacc gccgaggtac ccgtctcgat cgtctggggc accaaggatc cggtgctcgg
    22801 ccgcgtgatc aatcacctcg agcgcatgcg ccccgatgcg aaggtcgtgc gcaacgacgg
    22861 agccggccac ttcctccaag aagaagttcc ggacctcctc gccgacgcga tcatggatgt
    22921 ctcttcgcgc gcgagctggt cgtgaagacg aagttcgaag ttgccgccga cgacgaaggc
    22981 ctgcgcctgg atcaagtcat tcccaagcac atcgctggtc tgtcgcgccg caagacgcgc
    23041 gcagtgatcg atctcggtgg cgtgttcgtc gatcgaacgc gcgtgagggt tgcgggacgc
    23101 ctcgtcaagc gcgggcaagt catcgaggtc aacgtcggcg gcgcgatcga acgcgcgaac
    23161 gaggcgccac tcgcgccacg catcgtccac gtcgacgacc acatcatcgt catcgacaag
    23221 ccggcg
//
LOCUS       DATLBZ010000103         7556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8245654, whole genome shotgun sequence.
ACCESSION   DATLBZ010000103 DATLBZ010000000
VERSION     DATLBZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8245654"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1028)
                     /locus_tag="VMZ53_05440"
     CDS             complement(135..1028)
                     /locus_tag="VMZ53_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUS27926.1"
                     /translation="MGLGTAELFKKAKKIEIASRRLVDEQLAGQYHAVFKGRGLVFSD
                     VRPYYAGDDVRAIDWNITARMNAPHIKQFVEERDRTVNLVIDMSASGHFGSVGSTKRE
                     LAAELAAVVAFSAIKNNDRVGLFIVTDKIERYVPPKKGRRHVLRVIGEILAFEPTSRK
                     TDLAAGLDFLGKVAKRRSVVFFVSDFLGSDPMKWERSMRITAQRHELVPVVVADPLEQ
                     ALPAVGLLVLEDLESGEVIEVDTSSSVRADYARRAKAAAEARDTVLRRINLDVVAVNT
                     NEPYVDALVAFFRARAKRMGH"
     gene            complement(1037..2137)
                     /locus_tag="VMZ53_05445"
     CDS             complement(1037..2137)
                     /locus_tag="VMZ53_05445"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS27927.1"
                     /translation="MDTLRDPDVPGGLTLAEPAAASTSPFAPPPPPPPSAAPSVDITQ
                     LTEQVRKESAFLDDVFHEVGKVVVGQRDMVERVLIGLLTGGHCLIEGVPGLAKTLTVK
                     TLSQTLNATFSRIQFTPDLLPADITGTIIYNMHTGQFTQKRGPLFANLVLADEVNRAP
                     AKVQSALLEAMQERQVTIGDSTHPLPDPFLVLATQNPIEQEGTFPLPEAQLDRFMLLI
                     KVSYPTAGEERGMLDRMSSLDVPSPKEVATIDQLRQARRVVSSVYIDDRVRDYIVHIV
                     QATRKPRDYGLAELAPLIEYGASPRATLCLAAAARAHAFIRHRAYVTPDDIKAIALDV
                     MRHRVVVTYEAEAEEVSSEDVVRRIFEYLPVP"
     gene            2260..2538
                     /locus_tag="VMZ53_05450"
     CDS             2260..2538
                     /locus_tag="VMZ53_05450"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HUS27928.1"
                     /translation="MADQSMAPAPVDFSTHVMSLASTALIALGKMPAPDGESHPIDLE
                     TARHLIDVLAMLEAKTKGNLDEAETKLLASLVYDLRVAFVDAQKQGKG"
     gene            2535..3635
                     /locus_tag="VMZ53_05455"
     CDS             2535..3635
                     /locus_tag="VMZ53_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUS27929.1"
                     /translation="MRLVLAALLLLGCRERDLPQDASTSASSPLPGVATPAPSSTRIL
                     YPGGAPTGFVDLVARAKHGVVGIRAATPVKSGPAAMFPGAPETTADVALGTGFLIEAK
                     GVYVLTNDHIASASTDLEVVLPDRTTVKAKVLGRDTALDLALLSIDVPRLQPLPLGDS
                     NDIQVGEWLVVLGNPFGDDVTASAGIVSATGREGAGSLVAGRQHGFRTFIQTDARIHR
                     GNSGGPVLDTAGQVIGVAVATGDRPGEISFAIPIERVREVVGALRDVGAVTRSWLGAT
                     VLPVSNELAQQLSMQKATGALVTSVEPSSPAIRAALRAGDVILAWDDKPVDHRSLPWV
                     VSQTPVGKTVKLTIWRNKTQTDIAIVTEKMPQ"
     gene            complement(3641..4732)
                     /locus_tag="VMZ53_05460"
     CDS             complement(3641..4732)
                     /locus_tag="VMZ53_05460"
                     /inference="COORDINATES: protein motif:HMM:NF023645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HUS27930.1"
                     /translation="MIGWTIGCEIELLAPRGSSRRTLAEHYAKAADGAVRTFFHPQSE
                     VSLVPDLPVFENLTLGFEALDGRGDVIACCVDDLTLIDDLDREHPPQPGWFRVVSDDR
                     RLLELVVRTGRADAGPMEAVAPIAKLFGTEPEELTDGIIRVTDRMRSPIVIASRLPGE
                     RERPCEIVTPPIASDHEARLDALLGPARELGFTLPVEAATHIHFDGAPLRDARAFRNL
                     VRLVATWSPALKKLVGTNPRCRRLGGWPDALYEVVEAPDFAQLSWDEAKARLAEVGLS
                     KYCDVNVKNIVHDVPNKPTIEFRVLPGLRDTQPILAAAALFEGVLRLACEKRDFERER
                     VATNAKRLLDVLPLDEAVRKHWIAVARGS"
     gene            complement(4877..5722)
                     /locus_tag="VMZ53_05465"
     CDS             complement(4877..5722)
                     /locus_tag="VMZ53_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27931.1"
                     /translation="MVASAQDPTTTFLKQEKYTYCDVKILAEHWKTSAKEAKATIGTK
                     IEAGSDEYLRKEIYAGWQATKQKCTYVDGGFTFKDVQKLAKLWHITVAESKTTIENKL
                     KSGSSIYLKEEIRGSAESEKDPYTAFSSQTKYTYCDARMIGSLWGKSLAEGKKAIGQK
                     LVAKNTKGLTKLVADARKNAKKNVDARCSYDDAGLSYDDLVVLAKLWKKPEGDMKALV
                     AEKFALGQEATVLAQLKKEKAKQAKLKPVTPPPPAPMPPSNPAPKPPATKQATPPATA
                     PTKAD"
     gene            5799..6344
                     /locus_tag="VMZ53_05470"
     CDS             5799..6344
                     /locus_tag="VMZ53_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27932.1"
                     /translation="MRVRARSTALCVLPLAVMACTHERPITELHQVAGKRVTVETYDH
                     VSMEGVAIQAADGITFENSAGVMPPTAIAKVTEVRRGRGAAEGAGIGLAVGAVVGAAL
                     GFADGDDECDENSGMWCIFVFTAGEKAVMGGVVLGGIGAIVGLVAGAATGSKFVYSYG
                     DQVRITPTGPPGSYGGVTITY"
     gene            complement(6588..>7556)
                     /locus_tag="VMZ53_05475"
     CDS             complement(6588..>7556)
                     /locus_tag="VMZ53_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HUS27933.1"
                     /translation="GIGNILDAAKLAARGEVAGIGAAPRKKLQGFVELLAGLTDVIAQ
                     GASVAETIIQVVDRSGLRAKLEADESSESRDRLDNLAELVTIASDFDEESDEPQSVDA
                     FLERIALSAPSDQSAPQESVVLMTIHIAKGLEWPLVFITGMEDGLVPSLREREGVNED
                     TALEEERRLAYVAITRARERLVLTHARTRRVWGEIRMQGPSRFLDDLPASCLAGPVRP
                     KAMTPRAPMIVDGNWAGVRRRRPRASADEFDQRNHDDDVPTYHVDDDLAEPEVFRTGD
                     QVSHSMLGVGRVVAITDGKVIVEFPGAGRKTVLPKFLQHADDGSLN"
BASE COUNT         1202 a         2557 c         2465 g         1332 t
ORIGIN      
        1 accgccagag cccatcgcac cgcccgagcc catcgcactg ccggcaccgc ccgagcccat
       61 cgggtccgcg ccttggcccc acgcggtggc agccgcgaca agcgaaacga gcgcgatggc
      121 ggcggcgcgc ttcatcagtg ccccatcctc ttcgcgcgcg cgcgaaagaa cgcgacgagc
      181 gcatcgacgt acggctcgtt ggtgttcacc gccacgacgt cgaggttgat ccgccgcagg
      241 accgtgtcgc gcgcttcggc ggccgccttt gctcggcgag cgtagtcggc tcgcacgctc
      301 gacgacgtgt cgacctcgat cacctcgcca ctctcgagat cctcgaggac cagcaagccc
      361 accgcgggca gcgcctgctc gagcggatcc gcgacgacga cgggcaccag ctcgtgccgc
      421 tgcgcggtga tgcgcatcga gcgctcccac ttcatcgggt cggagccgag gaagtcgctg
      481 acaaagaaca cgacgctgcg gcgcttggcg accttgccga ggaagtcgag gccggcagcg
      541 agatcggttt tgcgcgacgt cggctcgaac gcgaggatct cgccgatgac gcgcagcacg
      601 tggcggcggc ccttcttcgg cgggacgtag cgctcgatct tgtcggtgac gatgaagagg
      661 cccacgcgat cgttgttctt gatcgccgag aacgcgacga ccgcggcgag ctcggctgcg
      721 agctcgcgct tcgtcgagcc caccgagccg aagtggcccg acgcgctcat gtcgatcacg
      781 aggttgaccg tgcggtcgcg ttcctcgacg aactgcttga tgtgcggcgc gttcatgcgc
      841 gcggtgatgt tccagtcgat cgcgcgaacg tcatcgcccg cgtagtacgg ccgcacgtcg
      901 gagaacacga ggccacggcc tttgaagacc gcgtggtact gacccgcgag ctgctcgtcg
      961 acgaggcgac gcgaggcgat ctcgatcttc ttcgccttct tgaagagctc tgcggtgccg
     1021 aggcccatgg ctcgccttac ggcaccggca ggtactcgaa gatacgccgc acgacgtcct
     1081 ccgagctgac ttcctcggct tccgcttcgt aggtcacgac gacgcgatgc cgcatgacat
     1141 cgagcgcgat cgccttgatg tcgtcgggcg tgacgtacgc gcgatggcga atgaacgcgt
     1201 gcgcgcgcgc ggctgcagcg aggcacagcg tcgcgcgcgg cgaggcaccg tactcgatga
     1261 gcggtgcgag ctcggcgagg ccgtagtcgc gcggtttgcg cgtcgcctgc acgatgtgaa
     1321 cgatgtaatc gcggacgcga tcgtcaatgt agaccgagct gacgacgcgg cgtgcttggc
     1381 gcagctgatc gatcgtcgcg acttccttcg gcgaaggcac gtcgagcgag ctcatgcgat
     1441 cgagcatgcc gcgctcttcg ccggccgtcg gatagctgac cttgatgagc agcatgaagc
     1501 gatcgagctg cgcttcgggc agcgggaacg tgccttcttg ctcgatcggg ttctgcgtcg
     1561 cgagcacgag gaacggatcg ggaagcggat gcgtcgagtc gccgatcgtg acctgacgct
     1621 cctgcatcgc ctcgagcagc gcagactgaa ccttggccgg cgcgcggttc acttcgtcgg
     1681 cgagcacgag gttcgcaaac agcggtccac gcttctgcgt gaactgcccg gtgtgcatgt
     1741 tgtagatgat cgtgcccgtg atatcggcgg gcagcagatc cggcgtgaac tggatgcgcg
     1801 agaacgtcgc gttcagcgtc tgcgacagcg tcttgaccgt cagcgtcttc gcgaggccag
     1861 gcacgccttc gatgaggcag tggccgccgg tgagcaaccc gatgagcaca cgctcgacca
     1921 tgtcgcgctg tccgacgacg accttgccga cctcgtgaaa gacgtcgtcg agaaacgcgc
     1981 tctctttgcg aacctgctcg gtgagctgcg tgatgtcgac cgacggcgcg gcacttggag
     2041 gcggcggtgg gggcggagca aacggtgagg tgctcgctgc agccggctcc gccagcgtca
     2101 gccccccagg cacatcggga tcccgcaacg tatccataga acctccctac ggtgtcacgg
     2161 attcccgccc cgtgcacccc gcccgcgaag cctcgactcc ggggacaaat cgcatcaact
     2221 acagcggttt cccggcggcc accttgttaa tgtccgcgca tggccgatca gtccatggcg
     2281 cccgcgccag tcgatttctc gacgcacgtt atgtcgctcg cgtcgacggc ccttatcgcg
     2341 ctcgggaaga tgcccgcgcc cgacggagag agccacccga tcgatctcga gaccgcgcgt
     2401 catctcatcg atgtcctcgc gatgctcgag gccaagacga agggcaacct cgacgaagcc
     2461 gagaccaagc tcctcgcgag cctcgtctac gatctgcgcg tcgcgttcgt cgacgcccaa
     2521 aagcaaggca agggatgaga ctcgtcctcg cagctctcct gctgctgggt tgtcgagagc
     2581 gcgatcttcc tcaggatgcg tcgacgtcag cgagctcgcc actgccggga gtcgcaacgc
     2641 cggcaccgag ctcgacgcgg atcctgtatc cgggtggcgc gccgactggc ttcgtcgatc
     2701 tcgtggcgcg cgcgaagcac ggcgtcgtcg gaattcgcgc tgcgacgccc gtgaagtcgg
     2761 gcccggccgc gatgtttcca ggcgcaccgg agacaaccgc cgatgtcgcc ctcggcaccg
     2821 gcttcctcat cgaagcgaag ggcgtctacg tgctgacgaa cgatcacatc gcgtcggcgt
     2881 cgacggatct cgaggtcgtg ctccccgatc gcaccacggt gaaggcgaaa gtcctcggcc
     2941 gcgacacggc gctcgacctc gcgctgttgt cgatcgatgt gccgcgcttg cagcccctcc
     3001 cgctcggcga ttccaacgac atccaggtcg gcgagtggct cgtcgtgctc ggcaacccat
     3061 tcggcgacga cgtgacggca tccgcgggca tcgtctcggc gacgggccgc gaaggcgcgg
     3121 gctcactcgt cgcgggtcgc cagcatggct tccgcacgtt catccagacc gatgcgcgca
     3181 ttcatcgcgg caactcgggc ggccccgtgc tcgacaccgc gggtcaagtc atcggcgtcg
     3241 cggtcgcgac cggcgatcgc cccggcgaga tctcgtttgc gattccgatc gagcgcgtgc
     3301 gcgaggtcgt cggcgcgcta cgcgatgtcg gcgcggtgac gcgcagctgg ctcggcgcta
     3361 cggtgctccc cgtctcgaac gagctcgcgc aacaactctc gatgcagaag gccaccggcg
     3421 cgctcgtcac cagcgtcgag ccgagctcgc cagcgattcg cgcggcgctg cgcgcaggtg
     3481 acgtcatcct cgcgtgggac gacaagcccg tcgatcatcg ctcgctgcca tgggtggtct
     3541 cgcagacgcc ggtcggcaag accgtgaagc tgacgatctg gcgcaacaag acgcagaccg
     3601 atatcgcgat cgtcaccgag aagatgccgc agtagccggc ttacgaaccg cgcgcgaccg
     3661 cgatccagtg tttgcgaacc gcttcgtcga gcggaagcac gtcgagcagc cgcttcgcat
     3721 tcgtcgcgac gcgctcgcgc tcgaagtcgc gcttctcgca cgcgagccgc agcacgcctt
     3781 cgaacagcgc ggcggcggcg aggattggct gcgtatcgcg caggccaggc agcacgcgaa
     3841 actcgatcgt cggcttgttg ggcacatcgt ggacgatgtt cttgacgttg acgtcgcaat
     3901 acttcgacag cccgacctcg gcgagtctcg ccttcgcttc gtcccatgac agctgggcaa
     3961 agtcgggcgc ctcgacaact tcatagagcg cgtcgggcca accgccgagc cgccggcagc
     4021 gcgggttcgt gccgacgagc ttcttcagcg ccggcgacca tgtcgccacg agccgtacga
     4081 ggttgcgaaa tgcgcgcgca tcgcgcagtg gcgcgccgtc gaagtggatg tgcgtggcgg
     4141 cttcgacggg aagcgtgaag cccagctcgc gcgcaggtcc gagcagcgcg tccagcctcg
     4201 cttcgtgatc gctcgcgatc ggcggcgtca cgatctcgca tggccgctcg cgctctcccg
     4261 gcaaccggct cgcgatcacg atcggagatc gcatgcgatc ggtgacgcgg atgatcccat
     4321 cggtgagctc ctcgggctcc gtgccgaaca gcttcgcgat cggcgcgacc gcctccatcg
     4381 gcccggcgtc cgccctcccc gttcggacga cgagctcgag cagccgccga tcgtcgctca
     4441 cgacgcgaaa ccagcctggc tgcggcggat gctcgcgatc caaatcgtcg atcagcgtca
     4501 gatcgtcgac gcaacacgcg atcacgtcac cgcgcccgtc gagtgcctcg aagccgagcg
     4561 tcaggttctc gaacaccggc agatcgggga cgaggctcac ttccgactgc gggtgaaaga
     4621 acgtccgcac ggcgccatcc gcggccttcg cgtagtgctc cgcgagcgtg cgacgcgagc
     4681 tgccacgcgg cgcgagcagc tcgatctcgc agccgatggt ccacccgatc accgacgcat
     4741 catcgctggt ccgggcgcat ttacacgcac tcgacccgca ctattcgcgc tgcgcgaaag
     4801 cgttagcagc ccacggcggc gattcaggct gggcgaacgc gcgcattttc atgggcgccc
     4861 atgattcgcg cgcggatcaa tcggctttgg tcggcgccgt cgctggcggc gtcgcttgct
     4921 tcgtcgcggg cggcttcggc gcaggattcg acggcggcat cggcgcaggt ggaggcggcg
     4981 tcacaggctt tagcttggcc tgcttcgcct tctccttctt gagttgcgcg agcaccgtcg
     5041 cttcctgacc gagcgcgaac ttctcggcga cgagcgcctt catatcgcct tcgggcttct
     5101 tccagagctt cgcgaggacc acgaggtcgt cgtacgacaa acccgcgtcg tcgtacgagc
     5161 agcgcgcatc gacgttcttc ttcgcgttct tgcgtgcatc ggcgaccagc ttcgtcaggc
     5221 ccttcgtgtt cttcgcgacc agcttctggc cgatcgcctt cttgccttcg gcgagcgact
     5281 tgccccacag cgagccgatc atgcgcgcgt cgcagtacgt gtacttcgtc tggctcgaga
     5341 acgccgtata cggatccttc tccgattcgg cggaaccgcg gatctcttcc ttgagataga
     5401 tcgagctgcc gctcttgagc ttgttctcga tcgtcgtctt cgattcagcg acggtgatgt
     5461 gccagagctt cgcgagcttc tgcacgtcct tgaacgtgaa gcccccgtcg acgtacgtgc
     5521 atttttgctt cgtcgcctgc cagcccgcgt agatctcttt gcgcaggtac tcgtcgctac
     5581 ccgcctcgat cttcgtgccg attgtcgcct tcgcttcctt cgcggaggtc ttccagtgct
     5641 cggccaggat cttgacgtcg cagtacgtgt acttctcctg cttcaggaat gtggttgttg
     5701 ggtcctgcgc actggcaacc acaggcagac ccaataccag tacagcaacc aacgacctca
     5761 cccggttcag catgtccttc ctatactacg attgaaaaat gcgcgtgcga gcccgcagta
     5821 ctgctctatg cgttttgccg cttgccgtga tggcgtgcac gcacgagcga ccgatcacgg
     5881 agctccacca ggtcgccggc aagcgcgtca ccgtcgagac gtacgatcac gtgtcgatgg
     5941 aaggtgtcgc gattcaagcg gccgacggca tcacgttcga gaacagcgcc ggcgtcatgc
     6001 cgccgaccgc gatcgcgaag gtcaccgagg tccggcgggg ccgcggtgcc gcggaaggtg
     6061 caggcatcgg gctcgccgtc ggcgcggtcg tcggcgctgc gctcgggttt gcagacggcg
     6121 acgacgagtg cgacgagaac agcggcatgt ggtgcatctt cgtgttcacc gccggcgaga
     6181 aggccgtcat gggcggcgtc gtgctcggcg gcatcggcgc catcgtcggc ctcgtcgcag
     6241 gcgccgcgac tggcagcaag ttcgtctact cgtacggcga ccaagtccgc atcacgccga
     6301 ccggaccgcc cggctcgtac ggcggcgtga cgatcaccta ctagctcgag ctccggatca
     6361 ttgaacaggg agacacggag cacacggagc tcggaaaagg aggggggctc gatagcgacc
     6421 gatcgatcga atgcacacgc ccgcccgcga accctcagca tcgaccttct gcttcggccc
     6481 tgcttccggc cttcgtgtgc tccgtgtctc cctgtttcat cgatcgggca cacgccctgc
     6541 cggtgcgcgt tctcgtcgaa tcgtcgtcgg tgcgcgggac tcggccgcta gttcagcgag
     6601 ccgtcgtcgg cgtgctgcag gaacttcggc agcaccgtct tgcgaccggc gccggggaac
     6661 tcgacgatga ctttgccgtc ggtgatcgcg acgacgcggc cgacaccgag catcgagtgg
     6721 ctgacttggt cgccggtgcg gaagacttcg ggctccgcga gatcgtcatc gacgtgatac
     6781 gtcggcacgt cgtcatcgtg gttgcgctgg tcgaattcgt cggcgctcgc gcgcggccgg
     6841 cggcggcgga cgcctgccca gttgccgtcg acgatcatcg gggcgcgcgg cgtcatcgcc
     6901 ttgggacgta cggggcccgc gagacagctc gccggaagat cgtcgaggaa gcgcgacggg
     6961 ccctgcatgc ggatctcgcc ccacacacga cgcgtccgtg cgtgcgtgag cacgagccgc
     7021 tcgcgcgcgc gcgtgatcgc gacgtacgcc agccggcgct cttcttcgag tgcggtgtct
     7081 tcattgacgc cttctcgctc ccgcagcgac gggaccaggc cgtcttccat tcccgtgatg
     7141 aacacgagcg gccactcgag gcccttcgcg atgtggatgg tcatgaggac gaccgactct
     7201 tgcggcgccg actgatccga cggcgcggac agcgcgatgc gctcgaggaa tgcgtcgacg
     7261 ctctgcggct cgtcggactc ctcgtcgaaa tcgctcgcga tcgtgacgag ctccgcgagg
     7321 ttgtcgaggc ggtcgcgcga ctcgctgctc tcgtcggcct cgagcttggc gcgcaggcca
     7381 ctgcgatcga cgacctggat gatggtctcg gcgaccgacg cgccttgcgc gatgacatcc
     7441 gtgagcccgg cgagcagctc gacgaagccc tgcagcttct tgcgcggcgc agccccgatg
     7501 cccgcgactt cgccacgtgc cgcgagcttg gccgcgtcga ggatgttgcc gattcc
//
LOCUS       DATLBZ010000104         2809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6756496, whole genome shotgun sequence.
ACCESSION   DATLBZ010000104 DATLBZ010000000
VERSION     DATLBZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6756496"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VMZ53_05480"
     CDS             <1..342
                     /locus_tag="VMZ53_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HUS27934.1"
                     /translation="IIHEHFEFSLAVGGKHSFFDVCKLGAKGNGVDDDRPFIQYAFNL
                     AAAAGRGEVYFAPGRTYRVSKYVDVAHASGLHIVGRGAVIRYPSDRDFGLRGMRRLCE
                     AGAARTIRSGA"
     gene            484..2766
                     /locus_tag="VMZ53_05485"
     CDS             484..2766
                     /locus_tag="VMZ53_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27935.1"
                     /translation="MKRVLAEVTKGRSLNEAIAKVLPARRRSWALRRIPNYREQGFEA
                     LIDARLPREANVAQACRQAVQAAREANPRITVDETLEILRKQAITPLPSESTIKREFA
                     RVKERRKYARKKAAAQGRTEVVELPFAGGELLAAAETETGGIAALTSEVVRIAEHALE
                     ASKGKTPAKDLANRNGDGHFTGRYNRARRRKRGEQIASYLRTAEEKAEGRVPSWPRFV
                     HEQPETIDAKLRMLVFAWMVVGSKGWDALRAPDVAGLASLTGFAYMPATLAKFVSALA
                     ISAADQPLIEATARHWHEVAQTRWGEPGAMAALYVDNHAKEVWSSLFTQSGKVSHLNR
                     VMPCITTTYAHTGAGTPVVLSVQSGGAPLAPRLVELVDQAETALETEIERAVVIDAEG
                     CTFDLLESFAKAKRVLITPLKPSRVPSLELTYTRGSYYRPYREHDELRIATATLVHKA
                     SGRTLEVGALLVRRAHRDADTVLLTTGLALGMEGQDLADLYYRRWPVQENAFKDGNAV
                     GLSEHRGNCGRIVANVAVVTELERLEARANRDRDALRTLADETASLEAVAAQQARDQA
                     RTESALATRRQRLDDAIAQGKTGGKTFARIALDHQQALVHTEAATQLAAKARTALDKN
                     RDRRAKLEQRAAEIADRRQLIEPRRTIRELDTTQDTILTATKLTALQLISFALREYLP
                     TMPMTPQTFLQRVLSVRGRKETSPDNELVVFYENFRDPLVNDALRDACQRLNRRKLER
                     GDRRLRFAVEPPPAIRERFD"
BASE COUNT          522 a          912 c          947 g          428 t
ORIGIN      
        1 atcatccacg agcacttcga gttcagtctt gcggtcggcg gcaagcactc cttcttcgac
       61 gtgtgcaagc taggtgcgaa ggggaatggt gtcgatgacg accgcccctt cattcagtac
      121 gcattcaacc tggccgcagc agccggccga ggagaggtct acttcgcgcc gggcaggacg
      181 tatcgggtgt caaagtatgt cgatgttgca cacgcatctg gtctccacat cgtcggacgg
      241 ggtgccgtga ttcgctatcc ctccgacagg gacttcggcc tgaggggaat gcgtcgattg
      301 tgcgaagccg gcgcggcaag gacgatccga tccggcgcgt agcccgttga ctcgccgatc
      361 gcggcgtgat caacccttgg tgtgtacgag ccgacgagcc tgacgctgat ccgtgtgcga
      421 tcgcgtgtga cggacgcgac gtggtcgcag cgggtcgagc aggcaaccgc cgatgaagtt
      481 gtggtgaagc gagtcctcgc cgaggtaacg aaggggcgct cgttgaacga agccatcgcg
      541 aaggtgctgc ctgcccgtcg ccgaagctgg gcgctgcgcc ggatcccgaa ctaccgtgaa
      601 cagggcttcg aagccctgat cgacgcgcga ctgccgcgcg aagcgaacgt cgcgcaggct
      661 tgtcggcaag ccgtgcaagc ggcccgcgaa gcgaatccgc ggatcaccgt ggatgagacg
      721 ttggagatcc tacgaaagca ggcgatcacg ccgctgccgt cggagtcgac cattaaacgc
      781 gagttcgcgc gggtcaagga gcggcggaag tacgcgcgca agaaagcggc ggcacaaggc
      841 cggaccgaag tcgtcgagct gccgttcgcc ggcggtgaac tgcttgcggc cgccgagacg
      901 gagaccggcg ggatcgcggc gctgacgagc gaggtcgtgc gcatcgccga gcacgcgctc
      961 gaagcctcga aggggaaaac gccggcgaag gatctcgcca accggaacgg cgatggccac
     1021 ttcacgggcc ggtacaaccg cgcgcgacgc cgcaagcgtg gcgagcagat cgcgtcgtac
     1081 ctgcgcacgg ccgaagagaa ggcggaaggg cgggtgcctt cttggccacg cttcgttcac
     1141 gagcaacccg agacgatcga cgcgaagctc aggatgttgg tgttcgcttg gatggttgtg
     1201 gggagcaagg gctgggacgc gcttcgcgcg cccgacgtgg cggggctcgc gtcgctcacg
     1261 ggcttcgcgt acatgccggc gacactggcc aagtttgttt cggcgctggc gatctccgcc
     1321 gctgatcagc cgctgatcga ggcgaccgct cgccactggc acgaggtcgc gcagacacgc
     1381 tggggcgagc cgggcgcgat ggcggcactg tacgtcgaca accacgcgaa ggaggtctgg
     1441 agctcgctgt tcacgcagag cggcaaggtg tctcacctca accgcgtgat gccgtgcatc
     1501 accacgacgt acgcacacac cggtgcgggc acaccagtcg tgctgtccgt gcagagcggt
     1561 ggggcgccgt tggcgccgcg cctggtggag ctggtcgatc aagcggagac cgcgctcgag
     1621 accgagatcg aacgcgccgt cgtgatcgat gccgaaggct gcacgtttga tctgctcgag
     1681 tcgttcgcga aggccaagcg cgtactgatc acgccgctca agccatcgcg cgtgccgtcg
     1741 ctggagctga cctacacgcg aggctcgtac tatcgcccgt accgcgagca cgacgagctg
     1801 cgaatcgcaa ctgccacgct cgtgcacaag gccagtggcc gtacgctcga ggtcggagcg
     1861 ttgctggttc gccgtgcgca tcgcgacgcc gacaccgtgc tcctcacgac cgggctcgcg
     1921 ctcggcatgg agggacagga tctcgcagat ctctactacc gccgatggcc ggtgcaggag
     1981 aacgcattca aggatggcaa tgcggtcggc ctcagcgaac atcgcggcaa ctgtggccgg
     2041 atcgtggcca acgtcgccgt ggtcaccgag ttggagcgac tcgaggcgcg cgccaaccgt
     2101 gaccgtgacg cactccgcac gctcgccgac gagacagcaa gcctcgaagc cgtcgcggct
     2161 cagcaggcac gtgatcaagc acgtaccgag tcggcgctcg cgactcggcg ccagcggttg
     2221 gacgacgcga tcgcgcaagg caagacgggc ggcaagacct tcgcgaggat cgcgctcgat
     2281 catcagcagg cgctcgttca caccgaggcc gcgacgcagc tcgccgccaa ggcacgcacc
     2341 gcgctcgaca agaaccgcga ccgccgtgcc aagcttgagc agcgcgcggc cgagatcgca
     2401 gaccgcaggc agctcatcga accccgacgc acaatccggg agctcgacac cacgcaagac
     2461 acgatcctga ccgcgaccaa gctcaccgcg ctccagctca tctccttcgc gttgcgcgag
     2521 tacctgccaa cgatgccgat gacaccccag acgttcctgc agcgcgttct gtccgtgcgc
     2581 ggccgcaagg agacgtcgcc cgacaacgag ctcgtcgtgt tctacgagaa ctttcgcgac
     2641 ccgctcgtca acgacgcgct tcgcgatgcc tgccagcgac tcaatcggcg aaagctcgag
     2701 cgaggcgacc gccggctgcg gttcgccgtc gagccaccgc ccgcgattcg cgagcggttc
     2761 gattgatctg atcctgcact gccgtgctgg ccgaagtccc tgtctgaag
//
LOCUS       DATLBZ010000105        13328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6756780, whole genome shotgun sequence.
ACCESSION   DATLBZ010000105 DATLBZ010000000
VERSION     DATLBZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13328
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6756780"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VMZ53_05490"
     CDS             <1..807
                     /locus_tag="VMZ53_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS27936.1"
                     /translation="SGTQDLIVNILAPGQRYEVANYPNVTIPTNIELKESARDKFGAF
                     YAALFDRTIEAHKGAVVTEYAWSAGSCDPCPGPTLDMSEIHTLGADVLEGPVDKPTPS
                     QSYDFVLTRLHARYGKDITNDLVFKQAKPILGGREDMGPEGVLQQGAYESGWNNFQAR
                     YIIRHPWTGPIRCITPHRGVWGGPPAEIAMKAEAGGVKPALDLAYAPRDLKLASVVAA
                     DVPAVGVTVAVEPGRTVAPGKAKSGCGCDTGGSSGGIVLLAFLALVRRRR"
     gene            804..2351
                     /locus_tag="VMZ53_05495"
     CDS             804..2351
                     /locus_tag="VMZ53_05495"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HUS27937.1"
                     /translation="MRKLLVLAALVAAPTAAHAFCGFYVAGGDQQMFNDATQVVLMRS
                     GTRTVLSMQNNYKGPPDAFAMVIPVPTVLHEGDVKTLPLDVFKHVESMGAPRLVEYWE
                     QDPCRPDFDRDDMVMKSGRAMGASVETKSAEKDFGVTIEAKFVVGEYQIVILSAKDST
                     GLDGWLRAEKYNIPPGAEPLLRPYVEGGMKFFVAKVDPKKVKFVDGRAALSPLRFHYD
                     TDQFNLPIRLGLANSSGTQDLIVNILAPHQRYEVANYKNVTIPTNINVKESVKDKFGA
                     FYTALFDATVEKNPGAVITEYAWQATTCDPCPGPALDYNDFTLLGSDVLKNDRSDRDG
                     DGFDFVLTRLHARYGKDITQDLEFKAADPIVGGREHVIDEKTGKLEEGAKKDSVNNFQ
                     GRYAIRHEWTGPIACQNPVRGRWGGQNGGNPTPQPAMDIAFAPRGQVKVAQVVKQDVP
                     ELGLKMGLEAALGEGKANPLPTPTTAPTEPKKAKTGCGCASTQGGDASGGLVLLALMG
                     LVRRRKR"
     gene            2348..3952
                     /locus_tag="VMZ53_05500"
     CDS             2348..3952
                     /locus_tag="VMZ53_05500"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HUS27938.1"
                     /translation="MRALLLAVALLAAPTTAHAFCGFYVAGSDKPMFNDATQVVLMRS
                     GTRTVLSMQNNYKGPPEAFAMVIPVPTVLHDGDVKILDPEVFEVVERMGSPRLVEYWE
                     QDPCRLEYPEEESGGTGTAMALDEGKMGKKDSETGVTVEAQFTVGEYNVVILSATDST
                     GLDTWLKREKYQIPKGAEPLLRPYVEGGMKFFVAKVDPKKVKFVDGRAALSPIRFHYD
                     SDTFTLPIRLGLANSSGTQDLIVNILARNQRYEVSNYKNVTIPTNITVKEGVKDKFGQ
                     FYVALFDATVEKNPGAVVTEYSWDASSCDPCPGPTLDYTDFETLGADVLKPDDGNMDE
                     GRMGGKGRRYYGGGFTLTRLHARYGKDIKDDLVFKEADPIVGGREFVVDPKTGKLEEG
                     ARKDDYNNFQARYAIRHPWTGPIACQNPVRGRWGAKDGGDAWSSPPPRRRQQGQYKME
                     KPDGPKPATDLAFAPRGMKLETAVTQDVPEIGLKAVAGAIVEGVPPKVDGKPGKKTGC
                     GCQSADASGGLVFFLGLWFATRRRRR"
     gene            3961..5550
                     /locus_tag="VMZ53_05505"
     CDS             3961..5550
                     /locus_tag="VMZ53_05505"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HUS27939.1"
                     /translation="MRALLVAGALLAVPATAHAFCGFYVAPKDGPLLADATQVVLMRA
                     GTRTVLSMQNNYKGPPEAFAMVVPVPVVLHEGDVKTLPKQVFENVERMGAPRLVEYWE
                     RDPCDPSGEGEEIAVRMYAPSAPRKKMAAKSEDLGVTIEAQFVVGEYQIVILSAKDST
                     GLDTWLKQEKYKIPDGAEPLLRPYVESGMKFFVAKVDPTKVKFVDGRAELSPLRFHYD
                     TPDFNLPIRLGLANSSGTQDLIVNILAPNQRYEVANYKNVTIPTNIDVKDSVKDRFGA
                     FYAALFDRTIEKNPGAVVTEYAWQATTCDPCPGPALNYHDFVTLGSDVITMGGDDDDM
                     PRKRGGGGRGSNFVLTRLHARYGKDIKDDLVFKAAEPIVGGREFVVDPETHKLEEGAK
                     PSGTNNFQGRYAIRHEWTGEIKCEKPQRGLWGGPHGSTRPETQPGLDLAFAPRGEVKL
                     ASAIVPKDVPEIGMTGGVLEVYTQPQAPAPGPAPIAPPPVEKGERGCGCQSSDPAGLL
                     GLLGSLLIFRPRTIRRRRKGR"
     gene            5547..7037
                     /locus_tag="VMZ53_05510"
     CDS             5547..7037
                     /locus_tag="VMZ53_05510"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HUS27940.1"
                     /translation="MKLSSAIAFGCALVAGSTAAHAFCGFYVSPSGEKMFADATEVVL
                     MRSGTRTVLAMQNDFKGPLEDFAMVVPVPVVLKEDDVRVLKKDIFERVDAMGSPRLVQ
                     YWEQDPCPMEWGTIGIGYGTVGYGSGMGASYGSIGIRSSSVKVEAKFDVAEYNIVILS
                     ATEAAGLQKWLEENKYKIPKGAEPLLRPYIEAGSKFFVAKVDPKKVEIGEDGHASLSP
                     LRFHYDSDEFSLPIRLGMANSSGKQDLIVNIISPDKRYEVSNYKNVFIPTNIDVKTTV
                     KARFAEFYTALFDATIAANPRAVVTEYSWTATPGAKCDPCPDNEMRANDVTILGGDVI
                     DGDVENKFVLTRLYARYGAKDMKDDLRFKEAKAVTGGREEWTKKGLEYGATPSEENFF
                     QARYAIRHWWTGPIKCKNPQRGVWGGPTGDEVDDGTISAQNLAFTPRGKLALATVIQR
                     DIWEIGYKKQAAPAKSKATVKTMGIGLGSLLLLVMGGVWIARRRKR"
     gene            7034..9124
                     /locus_tag="VMZ53_05515"
     CDS             7034..9124
                     /locus_tag="VMZ53_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HUS27941.1"
                     /translation="MRAALALVIVLAACGSQEGVARETKPAPRIDARLPRAPGLFGPS
                     LVALQGNREGDTSDGNMLSDVDSCSTCHPDAAAQWGTSPHSFASFGNPIYRVNVELAR
                     TELSKQASQHCGGCHDMPLMVDGLMAEQHVPASDLRAHSGVTCRLCHGIKSVSKDGNG
                     SYVWSRAPLDAPTLGDAASIETHRQQVSVKAVGTELCVGCHRGFLSPDMGQGMPVHLS
                     GLDEPGMWRNSAWAGNGMGRVDKVDKKTCIDCHMEREAASKDEYAAKAGTIASHRFLG
                     GHTWMASMRGDSEHLARMQAKLEGVASIDVQPSPTGHGFDVVIRNLLVGHRFPGGVLD
                     IQDTWIEVEARDASGKLVATSGLDHDKNAHDEDTHVLRTLVVDESGHILEKHEMPRFR
                     TQIAPQSLAAREAQAIRYALPADAKVSAPLTVTARLRHRSRTLQMQDEVCSASKTEEG
                     EAFLSGAKGAREVTLDPCRAQPITLIAQTTVTLGQPTTWEKQYEHGMALTAVISERLE
                     EARTVLDRALAAAPEGRPKAMVIVQLAQVASKQGRADDALALIARARKLLPEPGPAVL
                     DAVEADALARVWRWEEATMPAKRAAEKVKGNSQAWMVAARCFGSVADNEAALAAATAG
                     LELAPRDPDLLRSQATALSALGRPEAVDALAAYDRFRSPDASAELRISCAVDSLRCAR
                     EREQGHTHVLHLVR"
     gene            9132..10160
                     /locus_tag="VMZ53_05520"
     CDS             9132..10160
                     /locus_tag="VMZ53_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HUS27942.1"
                     /translation="MLFEQIRSGGCLSYLIGCDDSCAAMIVDPHLDQLDRYIALTAER
                     GMRVRYVAETHTHADHFSAARELGKRLDAKVVAHRLSAAPYVDVRVDDGETLVVGKLR
                     VRVLATPGHTNDSICLVFEDRVLTGDALLIGGTGRTDLPSGDPDTLYDSLFGKLLKLP
                     DTMKVYPSHDYKDRNSSTIGAEKASNPRLQKTERGEFVALMRGLDIRMPDHLTEALRT
                     NRSGGKTVAQLLAEASREIAFMSIEEVRDRIERHDPELVLLDVREKDSFVAGHLPGAK
                     HLPRGIIELSVDKALPDPSARILCYCNFGKISTLAAATLRSMGYTRAVALDGGYTDWV
                     KAGYPTES"
     gene            complement(10157..10927)
                     /locus_tag="VMZ53_05525"
     CDS             complement(10157..10927)
                     /locus_tag="VMZ53_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27943.1"
                     /translation="MVTLTACDGLFGLTHIRNTDAGQAIDAVDASRDAGDAVTPVQAV
                     QASSLAGNGTAPPLSIQFLSSTLTGNAVVLACAIGTSNTHLSGITDDSAGGANVWMRV
                     VDSSNWTSGPGVRLEVWYTTTGRPISSATVQYAITTDQRPLVCELIEWPRFAGLEGVN
                     VQPPSMNKQVIVSSGSITTTRPSVLVGIVGTADPVANAANVDAPFTSFGLGMTVNGTT
                     MITSDVVWAVRPPGPIEARWMLQFGQNWDAGLVAFGLR"
     gene            complement(10938..11324)
                     /gene="gcvH"
                     /locus_tag="VMZ53_05530"
     CDS             complement(10938..11324)
                     /gene="gcvH"
                     /locus_tag="VMZ53_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HUS27944.1"
                     /translation="MSNFPSDLKYTPDHEWLKAQGSSWRVGITQFAVDQLGDITLVDL
                     PKEGDQVTKGQRFGTVESVKSVSDLYAPVSGRVVSINAALKDSPEDVNGDPYGKGWMI
                     ELEANDKAEVDELMDATAYTAHVAAQ"
     gene            complement(11331..12449)
                     /gene="gcvT"
                     /locus_tag="VMZ53_05535"
     CDS             complement(11331..12449)
                     /gene="gcvT"
                     /locus_tag="VMZ53_05535"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HUS27945.1"
                     /translation="MAETGKKTPLWNAHKAAGAKLIDFGGWNMPVSYPAGTIKEHKAV
                     REAVGLFDVSHMGEAFLRGPRAREVVGKLTTNTCESAPAGKAVYTLLCRTNGGVVDDC
                     IFYKRADDDFMVIFNAGNIAKDLAWFREHAGTTCDVVDVSDETALIAVQGPKAVGMLD
                     KLTNADLAAMPSFTFKEATIAGVDCTAARTGYTGEDGFELACRNEDGPRLWAALMEAA
                     TPLGGLPIGLGARDSLRLESKLPLYGNDIDDDHTPLEAGLGWAVKLDKGDFLGAAALR
                     AQKAEGIKRQLVGFKIDEGARAIARHGYAVVDRSQGGDPVIGTVTSGGPGIVVGGAIG
                     LAYVPVAYAAAGSKLTIDCRGKDATATVVSGKFYKRPQ"
     gene            complement(12466..>13328)
                     /locus_tag="VMZ53_05540"
     CDS             complement(12466..>13328)
                     /locus_tag="VMZ53_05540"
                     /inference="COORDINATES: protein motif:HMM:NF025608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HUS27946.1"
                     /translation="MWFARFDPRDAARTFGVHKRFAVLQDQPLRIGDCIFDGGEETND
                     GECAAMGTLEAGDHTISWGLRWTRASSPITVYPDLAYRYSIGPTTFVTPNPSVPMSGE
                     LEIDGQRVTFDNVPMGQSHVWGKQHGYKWTWAHCSDFKDAPGAAFELIGARLHRRGVT
                     LPPLVMARLDLDGEKHELNQFRHVARNRATWGGTRLDFTAKNATLKIQGSFTCEPHEM
                     VVAPYLDPDGTEVFCSNTEIGTVRMTIFKRSGLSWREHRTLIGERRGHFEVGGRERDP
                     AITREHILVG"
BASE COUNT         2447 a         4207 c         4484 g         2190 t
ORIGIN      
        1 agcggcacgc aggatctgat cgtcaacatc ctcgcaccgg gccagcgcta cgaagtcgcg
       61 aactatccga acgtcacgat cccgacgaac atcgagctca aggaatccgc acgcgacaag
      121 ttcggcgcgt tctacgccgc gctgttcgat cgcacgatcg aagcgcacaa aggcgccgtc
      181 gtcaccgagt acgcgtggtc ggcgggcagc tgcgatccgt gcccgggccc gacgctcgat
      241 atgtccgaga tccatacgct cggcgcggac gtcctcgagg gtcccgtcga caaaccgacg
      301 ccgtcgcaga gctacgactt cgtgctcaca cgcctgcacg cgcgctacgg caaggacatc
      361 acgaacgacc tcgtgttcaa gcaagccaag ccgatcctcg gtggacgcga ggacatggga
      421 cccgaaggcg tactgcaaca aggcgcgtac gagtcgggct ggaacaactt ccaggcgcgc
      481 tacatcatcc ggcatccatg gacgggcccg attcgctgca tcacgccgca tcgtggtgtg
      541 tggggcgggc cgcccgcgga gatcgcgatg aaggcagagg cgggcggcgt gaagcccgcg
      601 ctcgatctcg cgtacgcgcc gcgtgatctg aagctcgcat cggtcgtcgc tgcagatgtt
      661 ccggccgtcg gcgtgacggt ggcggtagag cctggtcgca cggtggcacc gggcaaagca
      721 aagtctggct gtggttgcga caccggtggc tcgtcgggcg gtatcgtgct gctcgcgttt
      781 cttgcgttag tgaggaggag acgatgagga aactgctcgt tctggccgcg ctcgtggccg
      841 caccaacggc cgcgcatgcg ttctgcggct tctatgtcgc cggtggcgat caacagatgt
      901 tcaacgacgc gacgcaggtc gtgctcatgc gcagcggcac gcgaaccgtg ctgtcgatgc
      961 agaacaacta caagggccca ccggacgcgt ttgcgatggt gattccggtg cccaccgtgc
     1021 tgcacgaggg cgacgtgaag acgttgccgc tcgatgtgtt caagcacgtc gagtcgatgg
     1081 gtgcgccgcg cctcgtcgag tactgggagc aggacccgtg tcggcccgac ttcgaccgcg
     1141 acgacatggt catgaagtcg ggcagggcga tgggcgcgag cgtcgaaacg aagagcgcgg
     1201 agaaggactt cggcgtcacg atcgaggcga aattcgtcgt cggcgagtac cagatcgtca
     1261 tcctgtccgc gaaggattcc acgggactag acggctggct gcgcgcggag aagtacaaca
     1321 tcccgcctgg cgcggaacct ttgctgcggc cgtatgtcga aggaggcatg aagttcttcg
     1381 tcgcgaaggt cgaccccaag aaggtcaagt tcgtcgacgg ccgcgccgcg ctctcgccgc
     1441 tgcggtttca ctacgacacc gaccagttca atttgccgat ccgcctcggc ctcgcgaact
     1501 cgagcggcac gcaggatctg atcgtcaaca tcctcgcgcc gcatcagcgc tacgaggtcg
     1561 cgaactacaa gaacgtcacg attccgacga acatcaacgt caaggaatcg gtgaaagaca
     1621 agttcggcgc gttctacacc gcgctgttcg acgcgacggt cgagaagaat ccgggcgccg
     1681 tgatcacgga gtacgcgtgg caagcgacga cgtgtgaccc gtgtccggga ccggcgctcg
     1741 actacaacga cttcacgctg ctcggctccg acgttctcaa gaacgatcgg tcggatcgcg
     1801 atggcgatgg ctttgacttc gtgctgacgc gcctgcacgc gcgctacggc aaggacatca
     1861 cgcaggacct cgaattcaag gcggcggatc cgatcgtcgg cgggcgcgag cacgtcatcg
     1921 acgagaagac cggcaagctc gaagagggcg cgaagaagga cagcgtcaac aacttccagg
     1981 gtcgctacgc gatccggcac gaatggaccg gaccgatcgc gtgtcagaac cccgtgcgtg
     2041 gacgctgggg cgggcagaac ggcggtaatc cgacgccaca gcccgcgatg gacatcgcgt
     2101 tcgcaccgcg cggccaggtg aaagtggccc aggtcgtcaa gcaggacgtg cccgagctcg
     2161 ggctgaagat ggggctcgag gccgcgctcg gtgaaggcaa ggcaaacccg cttccgacgc
     2221 cgacgactgc gccgaccgaa ccgaagaaag caaagacggg ctgcggctgc gcgtcgacgc
     2281 agggtggtga tgcatcgggc ggcctcgtgc tgctcgctct gatgggactc gtgaggagga
     2341 ggaagcgatg agggcattac tactcgcggt ggcactgctc gctgcaccga cgacagcaca
     2401 cgcgttctgc ggcttttacg tcgcaggcag tgacaagccg atgttcaacg acgcgacaca
     2461 agtcgtgctc atgcgcagcg gcacgcgcac cgtgctgtcg atgcagaaca actacaaggg
     2521 gccaccggaa gcgttcgcga tggtgattcc ggtgccgacg gtgctgcacg acggcgacgt
     2581 gaagatcctc gatccggaag tgttcgaggt cgtcgagcgg atgggctcgc ctcgactcgt
     2641 cgagtactgg gagcaggatc cgtgtcgtct cgagtatccc gaagaagagt cgggcggcac
     2701 cgggacggcg atggcgctcg atgaaggcaa gatgggcaag aaggactccg agaccggagt
     2761 caccgtcgag gcgcagttca ccgtcggtga gtacaacgtc gtgatcctgt cggcgacgga
     2821 ctcgacgggt ctcgacacgt ggctcaagcg cgagaagtat caaatcccga agggcgccga
     2881 gccgctgctg cgtccatacg tcgaaggcgg gatgaagttc ttcgtcgcga aggtcgatcc
     2941 gaagaaggtg aagttcgtcg acggtcgcgc ggcgctctcg ccgatccggt ttcactacga
     3001 cagcgacacg ttcacgctgc cgatccgcct cggcctcgcg aactcgagcg gcacgcagga
     3061 cttgatcgtc aacatcctcg cgcgcaacca acgctacgag gtttcgaact acaagaacgt
     3121 cacgatcccg acgaacatca cggtcaaaga gggcgtcaaa gacaagttcg ggcagttcta
     3181 cgtcgcgctg ttcgatgcga ccgtcgagaa gaatcctggc gccgtcgtga ccgagtactc
     3241 gtgggatgcg agctcgtgcg atccgtgtcc gggcccgacg ctggactaca ccgacttcga
     3301 gaccctcggc gccgatgtcc tcaagcccga cgacggcaac atggacgaag gccgcatggg
     3361 cggaaagggc cgacgctact acggcggtgg cttcacgctg acgcggctgc acgcgcgcta
     3421 cggcaaggac atcaaggacg accttgtctt caaggaggcc gacccgatcg tcggcggtcg
     3481 cgagtttgtc gtcgatccga agacgggaaa gctcgaagag ggcgcgcgca aggacgacta
     3541 caacaacttc caggcgcgct acgcgatccg ccacccgtgg actggcccca tcgcctgtca
     3601 gaacccggtc cgcggacggt ggggcgccaa ggatggcggc gacgcatgga gctcaccgcc
     3661 gccgcgccgt cgacagcagg gccagtacaa gatggagaag cccgacgggc cgaagcccgc
     3721 gacggatctc gcgttcgcgc cgcgtggcat gaagctcgag acggcggtga cgcaagacgt
     3781 tccggagatc ggtctcaagg cggtcgccgg cgcgatcgtg gaaggcgttc caccgaaggt
     3841 cgacggcaag ccaggcaaga agaccggctg tggttgccag agcgccgacg catccggcgg
     3901 gttggtgttc ttcctcgggc tttggttcgc gacacgccgg aggcgacgat gagcgccgcg
     3961 atgagagcgc tgctagtcgc gggcgcgttg ctcgctgtgc ctgccactgc gcacgcgttc
     4021 tgcggcttct acgtcgcgcc gaaggacggg cctctgctcg ccgacgcgac gcaggtcgtg
     4081 ctcatgcgcg caggcacgcg caccgtgctg tcgatgcaga acaactacaa ggggccgccc
     4141 gaggcattcg cgatggtcgt cccggtgccg gtcgtcctgc acgaaggcga cgtgaagacg
     4201 ttgccgaagc aagtgttcga gaacgtcgag cgcatgggtg cgccgcggct cgtcgagtac
     4261 tgggagcgcg acccgtgcga tccatcgggc gaaggcgagg agatcgcggt gcggatgtat
     4321 gcgccgagcg cgccgcgcaa gaaaatggct gcgaagtccg aagacctcgg cgtcacgatc
     4381 gaggcgcagt tcgtcgtcgg tgagtatcaa atcgtcattc tcagcgcgaa ggactcgacg
     4441 ggcctcgaca cgtggctcaa gcaagagaag tacaagatcc ccgacggcgc cgagccgctg
     4501 ctgcggccgt acgtcgagag cggcatgaag ttcttcgtcg caaaggtcga tccgacgaag
     4561 gtcaagttcg tcgatggccg cgcggagctc tctccgctgc gcttccacta cgacacgccg
     4621 gacttcaact tgccgatccg tctcgggctc gccaatagct cgggcacgca ggacttgatc
     4681 gtcaacatcc tcgcgccgaa tcagcgctac gaagtggcga actacaagaa cgtcacgatc
     4741 ccgacgaaca tcgacgtgaa ggacagcgtg aaggatcgct tcggtgcgtt ctacgcggcg
     4801 ctcttcgatc gcacgatcga gaagaacccc ggcgcggtcg tcacggagta cgcgtggcag
     4861 gccacgacat gtgatccatg cccgggaccg gcgctcaact atcacgactt cgtcacgctc
     4921 ggctctgacg tgatcacgat gggcggtgac gacgacgaca tgcctcgcaa gcgcggcggc
     4981 ggcgggcgcg gaagcaactt cgtgctgaca cggttgcatg cgcgctacgg caaggacatc
     5041 aaggacgacc tcgtgttcaa ggcggccgag ccgatcgtcg gcggtcgcga gtttgtcgtc
     5101 gatcctgaga cccacaagct cgaagagggc gcgaagccgt ctgggacgaa caacttccag
     5161 ggccgctacg cgatccgtca cgagtggacc ggcgagatca agtgcgagaa accgcagcgc
     5221 ggtttgtggg gcggaccgca tggcagtacc aggcccgaga cgcagcccgg cctcgacctc
     5281 gcgttcgcgc cgcgcggtga ggtgaagctc gcctctgcga tcgttcccaa ggacgtcccc
     5341 gagatcggga tgacgggcgg cgtgctcgag gtctacaccc agccgcaggc accggcgccc
     5401 ggcccggcgc cgattgcacc gccaccggtg gagaagggcg agcgcggctg cggttgtcag
     5461 tcgagcgatc ccgcgggctt gctcggtttg ctcggctcgc tgctgatttt ccgacccaga
     5521 acgattcggc ggcgtcgtaa gggacgatga agctcagctc cgccatcgcg tttggctgtg
     5581 cgctcgttgc cggcagtacg gcagcgcacg cattctgtgg gttctacgtc agtccgtctg
     5641 gcgagaagat gttcgccgac gcgaccgagg tcgtgctgat gcgaagcggc acgcgcaccg
     5701 tgctcgcgat gcagaacgac ttcaagggtc cgctcgagga cttcgcgatg gtcgtgccgg
     5761 tgcccgtcgt gctcaaagaa gacgacgttc gcgtgctgaa gaaggacatc ttcgagcgcg
     5821 tcgacgcgat gggctcgccg cggctcgtgc agtactggga gcaggatccg tgtccgatgg
     5881 agtggggcac gatcggcatc ggctacggga cggtcggcta cggcagcgga atgggcgcgt
     5941 cgtacggctc gatcgggata cgcagctcct cggtgaaggt cgaggcgaag ttcgacgtcg
     6001 ccgagtacaa catcgtcatt ctctcggcga cggaagcggc gggcctccag aagtggctcg
     6061 aggagaacaa gtacaagatt ccgaagggcg ccgagccgct gctgcgtccg tacatcgagg
     6121 cgggctcgaa gttcttcgtc gcgaaggtcg acccgaagaa ggtcgagatc ggcgaggacg
     6181 gtcacgcctc gctgtcgcca ctgcgcttcc actacgacag cgacgagttc tcgctgccga
     6241 tccggctcgg gatggcgaac tcgtcgggca agcaggacct gatcgtcaac atcatctcgc
     6301 ccgacaaacg ctacgaggtc tcgaactaca agaacgtgtt cataccgacg aacatcgacg
     6361 tcaagaccac ggtcaaggca cggttcgccg agttctacac ggccctgttc gacgcgacga
     6421 ttgccgcgaa tccgcgcgcg gtcgtcaccg agtattcgtg gaccgcgacg ccgggcgcga
     6481 agtgcgatcc gtgtccggac aacgagatgc gcgcgaacga cgtcacgatc ctcggtggcg
     6541 atgtgatcga cggcgacgtc gagaacaagt tcgtgctgac gcgcctgtat gcgagatacg
     6601 gcgcgaagga catgaaggac gacctgcgct tcaaagaggc gaaggcggtc accggtggtc
     6661 gcgaggagtg gacgaagaaa gggctcgagt acggcgcgac tccgagcgag gagaacttct
     6721 tccaagcgcg ctacgcgatc cgccattggt ggacgggccc gatcaagtgc aagaacccgc
     6781 agcgcggcgt gtggggcgga ccaacgggcg acgaggtcga cgacggaacg atctcggcgc
     6841 agaacctcgc gttcacgccg cgcggcaagc tcgcactcgc aacggtgatc cagcgcgaca
     6901 tctgggagat tgggtacaag aagcaggcgg cgcccgcgaa gtcgaaggcg accgtcaaga
     6961 cgatgggtat cgggctcggc tcgctgctgc tgctcgtgat gggcggcgtg tggatcgcga
     7021 ggagacgcaa acggtgagag cagcgctcgc gctcgtgatc gtgctggctg catgtggcag
     7081 ccaagagggt gtcgcgcgcg agacgaaacc tgcgccgagg atcgacgcgc gactgccgcg
     7141 agcgccaggc ctctttggtc cgagcctcgt cgcgctgcag ggcaatcgcg aaggcgacac
     7201 tagcgatggc aacatgctgt cggatgtcga cagctgctcg acgtgtcatc ccgatgcagc
     7261 ggcgcagtgg ggcacgagcc cgcactcgtt cgcgtcgttc gggaacccga tctatcgcgt
     7321 caacgtcgag ctcgcgcgca cggagctcag caagcaggcg agccagcact gcggcgggtg
     7381 tcacgacatg ccgctcatgg tcgatggcct catggcggag cagcacgtgc cggcgagcga
     7441 tctgcgcgcg cattcgggcg tgacgtgccg gctgtgtcac ggcatcaaga gcgtcagcaa
     7501 agacggcaac ggtagctacg tgtggtcgcg cgcgccgctc gatgcaccga cgctcggaga
     7561 tgcggcgtcg atcgagacgc atcgccagca ggtcagcgtg aaggcggtcg gcacggagct
     7621 gtgcgtcggt tgccatcgcg gctttctctc gcctgacatg gggcagggca tgcccgtgca
     7681 tctctctggt ctcgacgagc ccggcatgtg gcgcaacagc gcgtgggccg gcaacgggat
     7741 gggacgcgtc gacaaggtcg acaagaagac gtgcatcgat tgccacatgg agcgcgaggc
     7801 agccagcaag gacgagtacg ccgccaaagc cggcacgatc gcatcgcatc ggttcctcgg
     7861 cggtcacacg tggatggcgt cgatgcgcgg cgacagcgag cacctcgcgc ggatgcaggc
     7921 caagctcgag ggcgtcgcgt cgatcgacgt gcagccatcg ccgacggggc acggctttga
     7981 cgtggtgatt cgcaacctgc tcgtcggtca tcgctttccc ggcggcgtgc tcgatatcca
     8041 agacacgtgg atcgaggtcg aagcgcgcga tgcgagtggc aagctcgtcg cgacatcggg
     8101 tctcgatcac gacaagaacg cgcacgacga agacacgcac gtgctgcgca cgcttgtcgt
     8161 cgacgagagc ggccacatcc tcgagaagca cgagatgccg cgctttcgca cgcagatcgc
     8221 gccgcagtcg ctcgccgctc gcgaggcgca agcgattcgc tacgcactgc ccgcagacgc
     8281 gaaggtcagc gccccgctca ccgtgaccgc gcgccttcgt caccgcagcc gcacgctgca
     8341 gatgcaggac gaagtctgct cggcctcgaa gaccgaagag ggcgaggcat tcttgtcggg
     8401 cgcgaagggc gcgcgtgaag tgacgctcga cccgtgcagg gcgcagccga tcacgctgat
     8461 cgcgcagacg accgtcacgc tcgggcagcc gacgacgtgg gagaagcagt acgagcacgg
     8521 catggcactg accgcagtga tcagcgaacg actcgaggaa gcgcgcacgg tgctcgatcg
     8581 tgcgctcgcc gctgcgcccg aaggcagacc aaaggcgatg gtgatcgtgc agctcgcgca
     8641 ggtagcctcg aagcaaggtc gcgcggacga cgcgctcgcg ttgatcgctc gagcgcgcaa
     8701 gctcttgccc gagcccgggc ctgccgtgct cgatgccgtc gaggccgatg cgctcgctcg
     8761 ggtctggcgg tgggaagaag cgacgatgcc ggcaaagcgc gcggccgaga aggtgaaggg
     8821 gaactcgcag gcgtggatgg tcgcggcgcg ctgcttcggt tcagtcgccg ataacgaagc
     8881 cgccctcgcg gcagcgaccg ccgggctcga gctcgcgcct cgcgatcccg acctgttgcg
     8941 aagccaagcg acggcgctgt ccgcgctggg gcgccccgag gctgtcgatg cattggctgc
     9001 gtacgaccgc ttccgctcgc ccgatgcgtc cgcggagctg cggatttctt gtgccgttga
     9061 ctcgctgcgc tgcgcgcgcg aacgcgaaca aggccacacg cacgtgctcc acctcgtgcg
     9121 ataacggctc catgctgttc gagcagatcc gttctggcgg atgtctgtct tacctcatcg
     9181 gttgcgacga ttcgtgcgcg gcgatgatcg tggatccgca tctcgatcag ctcgatcgat
     9241 acatcgcgct cacggccgag cgaggcatgc gcgttcgcta cgtcgcagag acgcacacgc
     9301 atgccgatca cttctccgct gcgcgcgagc tcgggaagcg tctcgatgcg aaggtcgtgg
     9361 cgcaccggct gtccgcggcg ccctacgtcg atgttcgcgt cgatgatggc gagacgctcg
     9421 tcgtcggcaa gctgcgcgtg cgcgtgctcg cgacgcctgg ccacacgaac gattcgatct
     9481 gcctcgtgtt cgaggatcgc gtgctcaccg gcgacgcgct gctcatcggc ggcaccggcc
     9541 gcaccgattt gccatcgggt gatcccgaca cgctgtacga cagcctgttc ggcaagctgc
     9601 tcaagctgcc cgacacgatg aaggtctatc catcgcacga ctacaaggat cgcaattcgt
     9661 cgacgatcgg cgccgagaag gccagcaatc cgcgtctgca gaagacggag cgtggcgagt
     9721 tcgtcgcgct gatgcgcggt ctcgacatcc gcatgccgga tcacctgacc gaggcgctgc
     9781 gcacgaatcg ctcgggcggc aagaccgtcg cgcagctgct cgccgaagcg tcgcgcgaga
     9841 tcgcattcat gagcatcgag gaagttcgcg accgcatcga gcggcatgac cccgagctcg
     9901 ttctcctcga cgtccgcgag aaagactcgt tcgtcgcggg ccacctgccc ggtgcgaagc
     9961 acctgccgcg cggcatcatc gagctctcgg tcgacaaggc gctccctgat ccgagcgcgc
    10021 gcatcctctg ctactgcaac ttcggcaaga tctcgacgct ggcggcagcg acgctgcgct
    10081 cgatgggcta cacgcgcgcg gtcgcgctcg acggtggcta taccgattgg gtcaaagccg
    10141 gctatccgac cgagagctag cgaagaccaa acgccacgag cccggcatcc cagttctggc
    10201 cgaactggag catccatctc gcctcgatcg gtccaggcgg tcggacggcc cacaccacat
    10261 cggaggtgat catggtggtc ccgttcacag tcatgccgag gccaaagctc gtaaatggcg
    10321 cgtccacgtt cgccgcgttc gcgacggggt cggcggtgcc gacgattccg acgaggacag
    10381 atggtcgcgt cgtcgtgatc gagccgctcg acacgatcac ctgtttgttc atcgacggcg
    10441 gttgcacgtt cacgccctcg agaccggcaa atcgcggcca ctcgatgagc tcgcagacga
    10501 gcggccgctg atcggtggtg atcgcatact ggaccgtcgc gctgctgatc ggacgaccgg
    10561 tcgtcgtgta ccagacttcg aggcggacac ccggcccact ggtccagttg gaggagtcga
    10621 cgacgcgcat ccagacgttc gcaccgcctg cgctgtcgtc ggtgatgccc gacaaatgcg
    10681 tgttgctcgt cccgatcgcg cacgcgagca cgaccgcgtt gccggtcaac gtcgagctca
    10741 ggaattggat gctcagcggc ggcgcggttc cgttgccagc gaggctgcta gcctgcacgg
    10801 cttgcactgg agtgaccgcg tcgccggcat cgcggctcgc gtcgaccgca tcgatcgcct
    10861 gacctgcgtc ggtgttgcga atatgcgtga gtccgaacag cccatcgcag gccgtcagcg
    10921 tgaccaatag aagaacgcta ctgcgccgcg acatgcgccg tgtacgccgt cgcgtccatc
    10981 agctcatcga cttcggcttt gtcgttcgcc tcgagctcga tcatccagcc cttgccgtat
    11041 ggatcgccgt tgacgtcctc gggcgagtct ttgagcgcgg cattgatcga cacgacgcgg
    11101 cccgagacag gcgcgtagag atcgctgacg ctcttcacgc tctcgacggt gccgaagcgt
    11161 tggcctttgg tgacctggtc gccttctttc ggaagatcga cgagcgtgat gtcgccgagc
    11221 tgatcgaccg cgaactgcgt gatgccgacg cgccaggacg agccctgcgc cttcagccat
    11281 tcgtggtcgg gcgtgtactt cagatcggag ggaaagttgc tcatgactac ctactgaggc
    11341 ctcttgtaga acttgcccga aaccacggtc gccgtcgcgt ccttgccacg gcaatcgatc
    11401 gtgagcttgc tgcccgccgc ggcgtacgcg acagggacgt aggcgagacc gatcgcgccg
    11461 ccgacgacga tgccgggacc gccgctcgtc acagtgccga tgacgggatc gccaccttgc
    11521 gaacgatcga cgaccgcgta gccgtgacgc gcgatcgcgc gagcgccttc gtcgatcttg
    11581 aagccaacga gctggcgctt gatgccttcg gctttttgcg cgcgcagcgc ggcggcgccg
    11641 aggaagtcgc ccttgtccag cttgaccgcc cagccaaggc cagcttcgag cggtgtgtga
    11701 tcatcgtcga tgtcgttgcc gtagagcggc agcttcgact cgagccgcag tgaatcgcgc
    11761 gcgccgaggc cgatcggcag gccgccgagt ggcgtcgcgg cttccatgag cgcagcccac
    11821 agtcgcgggc catcctcgtt gcggcatgcg agctcgaagc catcttcgcc ggtgtaaccc
    11881 gtgcgcgcgg ccgtgcagtc gacgccggcg atcgtcgctt ccttgaacgt gaagctcggc
    11941 atcgccgcga ggtctgcgtt cgtcagcttg tcgagcatgc cgacggcttt cggtccctgc
    12001 accgcgatga gcgcggtctc gtcgctgacg tcgacgacat cgcacgtggt cccggcgtgc
    12061 tcgcgaaacc aagcgaggtc cttcgcgatg ttgcccgcgt tgaagatgac catgaagtca
    12121 tcgtcggcgc gcttgtagaa gatgcagtcg tcgacgacgc cgccgttggt gcggcacagc
    12181 agcgtgtaga ccgccttgcc agcgggcgcc gactcgcacg tgttcgtcgt gagcttgccg
    12241 acgacctcgc gagcgcgcgg gccgcggaga aacgcttcac ccatgtgcga gacgtcgaat
    12301 aggcccacgg cttcgcgcac ggccttgtgc tctttgatcg tgccggctgg atacgagacg
    12361 ggcatgttcc agccgccgaa gtcgatcagc ttcgcgcctg ctgctttgtg cgcgttccag
    12421 agcggtgtct ttttgcccgt ctcagccatc gcggcgaaca ctacctcagc cgacgaggat
    12481 gtgctcgcgc gtgattgcag gatcgcgctc gcggccgccg acctcgaagt gcccgcgtcg
    12541 ctcgccgatg agcgtgcggt gctcccgcca gctgaggccc gagcgcttga agatcgtcat
    12601 gcgaacggtt ccgatttcgg tgttcgagca gaagacttcg gtgccgtcgg gatcgagata
    12661 cggcgcgacc accatctcgt gcggctcgca ggtgaacgag ccctggatct tgagggtcgc
    12721 gttcttcgcc gtgaagtcga ggcgggtgcc gccccacgtg gcgcggttgc gtgcgacgtg
    12781 acggaactgg ttgagctcgt gcttctcgcc gtcgagatcg agccgcgcca tgacgagcgg
    12841 cggcagcgtc acaccacggc gatgcaggcg cgcgccgatc agctcgaaag cagcaccggg
    12901 tgcgtccttg aagtcgctgc agtgcgccca ggtccacttg tagccgtgct gcttgcccca
    12961 cacatgcgac tggcccatcg gcacgttgtc gaacgtgacg cgctggccgt cgatctcgag
    13021 ctcgccggac atcggcacgc tcgggttcgg tgtgacaaac gtcgtcggtc cgatcgaata
    13081 gcgatacgcg agatccggat acacggtgat cggggagctc gcgcgcgtcc atcgcaggcc
    13141 ccacgagatc gtgtgatcgc cagcttcgag cgttcccatc gcggcgcact cgccatcgtt
    13201 cgtttcctcg ccaccgtcga agatgcagtc gccgatgcgc agcggctgat cctggagaac
    13261 ggcgaagcgc ttgtgcacgc cgaacgtgcg cgcagcgtcg cgcggatcga aacgcgcgaa
    13321 ccacatct
//
LOCUS       DATLBZ010000106        15669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10484513, whole genome shotgun sequence.
ACCESSION   DATLBZ010000106 DATLBZ010000000
VERSION     DATLBZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15669
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10484513"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VMZ53_05545"
     CDS             <1..372
                     /locus_tag="VMZ53_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27947.1"
                     /translation="FGDKADATDELWELRASSDHWALVCGPTQKGCAWEPRSEASLAY
                     LAATDSTFAIGGAGGNPRHEFKGAWILDDNEFIHSHDDPPARDFASMSYDPERKRIVV
                     YGGNGVGCAGDCDGTWILESD"
     gene            complement(369..830)
                     /locus_tag="VMZ53_05550"
     CDS             complement(369..830)
                     /locus_tag="VMZ53_05550"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HUS27948.1"
                     /translation="MYQLAQANVAYALCDADDPRIAEYIARLDEMNQLADASPGFVWR
                     YLTDSRDPAQREFPDPRVLFNLSVWESIEALHTYTYRTAHAEVYAARKRWFVDEKAVV
                     GGKALAMWWIPRGELPTPAEAKARLEAIEANGPTEHAFTFKRRFPPPSLAR"
     gene            complement(893..1834)
                     /locus_tag="VMZ53_05555"
     CDS             complement(893..1834)
                     /locus_tag="VMZ53_05555"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HUS27949.1"
                     /translation="MASSRITQFLDGFPVVLAPMEDVTDAAFRRVARSLGASLCMTEF
                     IGVEQLIAGSKLAQKRARLAPDDRPTAIQIYGADPDLLVAAARIAEAAEPSFIDINCG
                     CWIPKIARRGAGAGWLRDPAAMVEMVRRITATVSLPVTVKTRIGWGPESHMPIVDLAR
                     RLEDAGIEALTIHCRTAAMGHNGPADWSWAARAQAVVGIPVIVNGDVRTADDVVTALA
                     ETGCAAVMVGRAAIDRPWIFREARARLAGEPIEPPTDDERRAVYRALLDASIATRGER
                     GAVHSTKRHIRVLAPSATMQQRVLRAQDLSEAYAAIA"
     gene            complement(1839..2708)
                     /locus_tag="VMZ53_05560"
     CDS             complement(1839..2708)
                     /locus_tag="VMZ53_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27950.1"
                     /translation="MRALLVLSVAAACGGESTDPTPDAAIGSSFQTLITSSWSLQPGT
                     ELYQCATVTVPRDMYITELRPMIPIGTHHTVMSIGTVRGPDNAGYECSNPFEFGGRFI
                     YGTGVGSKPFQYPEGVALKLSAGQQVHINLHLFNTGDAVLSGTSGVEVRETEASKVQN
                     QARMELNGPTDTLNIPTGVSTQTGTCTALSEINVVSFQPHMHQLGTHLTYTVTPTVGE
                     AYKLYDQDYTFDGQEHVMFDPIRTLHKGDVLKIECSYNNTTGSAVGFGESTKDEMCFA
                     GMTIYPSTTTFCQ"
     gene            complement(2734..3447)
                     /locus_tag="VMZ53_05565"
     CDS             complement(2734..3447)
                     /locus_tag="VMZ53_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27951.1"
                     /translation="MKSRLALLGGGIALGAVLFYALHGDDSNAQTTSSSTERTADRSR
                     AQARSAGPNETGQLAAPGVVKHADTVEHANVDGLPEELEPGYLAIEHAAKGCYRGRPA
                     PTPIRPAGPDETIESIRLSYRQVANHGVGRVENLKVNDGELTDKALQACIVKAAAGAT
                     WSSTEPDGVIGDLEQNIMLGDLLRPDHGLPPVDKRSPTPTAPPPPELAGPLGQQAPEP
                     EDDHADVIVADPSVAKQRK"
     gene            complement(3454..5022)
                     /locus_tag="VMZ53_05570"
     CDS             complement(3454..5022)
                     /locus_tag="VMZ53_05570"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HUS27952.1"
                     /translation="MVFLALALPSAAGAAGYMNAGYMNAGYMNAGYMNAGYMNAGYMN
                     AGYMNAGYMNGGSIGGFNLGPMMTAQGQPIDNVVVTKDYLVGSANVWEFDHFGPNWAP
                     VNAWHYRTIEKDEFLNATFEATINDADTDQLAGYVTVKITAIKTIAGADGTTHYQHVV
                     KWKDAQTGALTPLCGCENANPNCTAPVPATFTQGMWNYTQGTIDGGKRISGSASLLTV
                     ACAGGAISKCTSDQATTSPLTPSYSGSVSVPAYSWSHKGSWIVYPGEKITVKLSNVTG
                     DPNLYVRWNNQATASTYSCRPNLGEAVTETCTLTVPSSAYLAYISVYGEHSTTSSATL
                     TVSASNAAPRGLGYYGYGSTMLCTYNYMTGTSSCNTSPTTRIQSCTRMVTADYCGDGT
                     PHTVTGRPIEVYDNFTPVVNDPDPDSTAYYFESEWTTTGANSIGTCRVDDMDLSAADP
                     AACPVYNGTQHQIAHYTMVYNNYGQPIGCAANQQNSGVYTNRAGYLYNYNDGTFYPRQ
                     LRVSGYTMMPIVLR"
     gene            complement(5179..9156)
                     /locus_tag="VMZ53_05575"
     CDS             complement(5179..9156)
                     /locus_tag="VMZ53_05575"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27953.1"
                     /translation="MARRWTPPQQEFDEYKLVKHLGAGAMGDVFLAHDRLLDRPVAVK
                     FIAADLEADVGARERFFVEARAAARLQHPNVVAVYRVGELEQRPYLISEFVRGERLDR
                     VPRPMPWQRALELAIGLARGLAAAHRRGVLHRDIKPANTVLTENGDIKLLDFGLAKLI
                     DAAVPEEPPPEPAKRARSPVVIVDAGGGHDDTVLPAHATPPLGVATPIPTTAGNGRSV
                     SHEDTVPPVGAKMPPPAAKPAAAFDPDDTRQIATPVPTTARDSSPSLTDSNPSLTRAG
                     AVMGTPYYMAPEVWRGEVATRRSDVYSLGALIYELCAGQPPSKDIPYPQLMVEVQKRE
                     TPPLLSKAPTVDSRLAAVVDRCLRRDPAERYESAEQLRDALEQLAPSQVAKAVPEGNP
                     YRGLAAFESEHRAFFFGRDAAIRGVIDRLRVEPFILVAGDSGVGKSSLCRAGVLPLVE
                     EGGFGPDRTWTTLKLMPGKHPVATITAALAPMLGKGEGALAELLVRDPASLARELRAL
                     QGADRGLLIFIDQLEELVTLAEPAQAAAVAELIVRLNIPSPGVRLLCTVRGDFLTRLA
                     RLPALGDEVTRALYLLGPLTPEGVREAIVGPAQIKGVRFENEETIEQLIDEAADTHLP
                     LLQFVLAELWDARDTKANVITAESLEARGGLAGALTREADGVLERLLPAHRAAARRVL
                     LQLVTTEGTRARVAETELVGDDSNARAAVDALVRGRLLVAREDADGTAYEVAHEALIQ
                     EWRTLRGWIHDDADRRVIKERLARAAAEWQRLGRPREGLWSTRQLAEAEKLDTLSARD
                     QEFVDQSRRTIARSRIVRWSLAVGVVLALASVYLIVRLSEQNRRARRVDEELVIARDA
                     LAKARRLATDLGNVRVQAFASFDANHYEDGEKQWAEARKLATKTDRAFGDASRAGETA
                     LMIDPSRADARGVLADVIYDRALLAERDQRPEQLVELLARLDLYDVSGTRRAAWSAPG
                     HVRLDSQPAGAQIAVERWLDGKWAPLGKPQPAPYDAGLPHGSYRFTFALAGRPPVHYP
                     VLLGRGVTVTEVVSLPDKVPDGFAYVPPARVLIGSTAEEGHRRDFQHAPPRHEMRVGG
                     FFISRTELTFDEWIRWLDTLPDGEREKRSPRVQTFGVQGYVRLERISGRWRLTLQPSA
                     TARLEAWDGEPITYPGRSRNAVVNWRRLPVSGISQQDAIEYAAWLDRSGYVPGARLCS
                     EDEWEAAARGADGRMYAIGDDVRPENGNWQFTYGGAVDSGPDEINLENPEVARTVYGL
                     DGLVGNVFDMTTANLQGVPGVTASGRGGAFGFDLGAVKIEQHNQVEASYRDIGLGVRL
                     CASLR"
     gene            complement(9159..11075)
                     /locus_tag="VMZ53_05580"
     CDS             complement(9159..11075)
                     /locus_tag="VMZ53_05580"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS27954.1"
                     /translation="MSTSSGDSTDRGRKTPADWDESTIAPSGASIGQPHGDAGQPLGD
                     QAYLLVFEGASSSMFSLPAAGDVTIGRGEGATLRLSDTSVSRMHAQVTMAAGEARICD
                     LESQNGTLVNGERITGTRRLFTGDTISICSISIVFHASVRMPAGVHRPILKFEEMRRR
                     TEEEIERALHYHRPLTTLVAILQIEPSHRPRVAHALGASLRILDVVGSGASDQLIILM
                     PECDVDLAPQAAARVLKSLVPIAPGAKVGFATCPHDGCDVETLVSSAREAALAADAGN
                     VGAAARTYETKRIADHTVIVADPAMSRLYALIERLAAAELPVLICGETGTGKELAARA
                     VHQWSARKAKSLVAFNCAALSENLVESELFGHEKGAFSGAVAAKSGLLEVASGSTVFL
                     DEIGEMSPTTQAKLLRVLETKRVIRVGDVREREIDIRLVAATNRNLADEVKAGRFRQD
                     LFFRLSAATLWLPPLRDRRRELAILAQTFLAEACKQANRATMEISDDAMQRLATYEWP
                     GNVRELKNVMDYVAAAFTEPVLQAWHLSRSLSGPSEGDEDEELQAAAPKRGRTNTPLS
                     IPIVGSDSVSPTKFRPIEDEIKDLERRRMIEALAAAGGNQTRAADLIAMPLRTFQAKL
                     RFYDIPRPGQKSRG"
     gene            complement(11221..11490)
                     /locus_tag="VMZ53_05585"
     CDS             complement(11221..11490)
                     /locus_tag="VMZ53_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27955.1"
                     /translation="MGLMGDGYRGTYQGHRIELVRDNLAKKLKLVIDGVEVASEPLVM
                     QHDITLTATLVHEAVSHRVVARSIVGRTTSGTVTIDGAPLTIAKT"
     gene            complement(11501..12877)
                     /locus_tag="VMZ53_05590"
     CDS             complement(11501..12877)
                     /locus_tag="VMZ53_05590"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HUS27956.1"
                     /translation="MLKTYAAQLCVVVVVAFVFGCGQVTGQKTDAGGSGGDGQMGCTT
                     TAQCSGATPVCNTSSMVCVACTASEIGACAGTTPVCATDNTCRACEAHSDCASNACLP
                     DGSCGTDTNVAFVDASAVGSTQCTRAMPCPQISAALATNRPYIKVHGTIDESVSLTNR
                     NVTFLADPGAILKNSGNNGLTLGGSSHVEIYDLTISNSVTGINVPTGTTVSLFLSRAK
                     VQNNANGGIVMESGTLTVTRSTIANNTGAGGIFSHNSVVSVTASTLSGNSGGEAIIYY
                     AGSLSVSRSTFVNNINGGISVSPTGTFSIVGNVFFGNGQTSGTTGGVTINTAQSAGNR
                     LELNTFVGNRTSTSNAAAIQCTAGTFTARSNIMSGNMGGANQFSGTCLHAYSISRPGA
                     VPTGTGNIATDPMFVDDAAGNLHLKAGSPARGAGDPASDLTGIAALDIDGDMRVSPPD
                     IGADQFKP"
     gene            13034..13696
                     /locus_tag="VMZ53_05595"
     CDS             13034..13696
                     /locus_tag="VMZ53_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27957.1"
                     /translation="MKRRLLISLIAAGAALGSGVASANASYWAQEGLLLEVSADPRDG
                     SDFVQTNPVHAQSLELVALNDNVRLRGMTVRFTDGRTFSQDMRTIRAGEHVRIDLPEN
                     CGTIQGVQLDYGQQLVDRTTARLQIIPREDRQIAALPSRPVIRDHRWSRHEYRYIAPQ
                     PAYVAPQPTYVAPQPTYSYQQPTYTYQPSSYHTTPTYVVPAPRPQSSIGISGYIQGSF
                     RF"
     gene            complement(13778..14101)
                     /locus_tag="VMZ53_05600"
     CDS             complement(13778..14101)
                     /locus_tag="VMZ53_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27958.1"
                     /translation="MHKDSFETISAGELERVGGGFDIGALMQGIGSFLGPKGQAITQG
                     ISGIWQSIQGMIGAFQGGGQQQQPGGGEQQQAPQQQMQQMGGGMCCGQMGGGQMGGGQ
                     MGGGQ"
     gene            14221..15273
                     /locus_tag="VMZ53_05605"
     CDS             14221..15273
                     /locus_tag="VMZ53_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27959.1"
                     /translation="MKRALVSVGLVLTGCGNDPCDPAAESCTYEADVSTLTIGGGVED
                     EDTCQSWTLNNPTELWVTSIAQSNDGAYHHANWFFVPDDQYKLPDGTWACGANQFEEL
                     VAALQGGYLFALSTQSHNEQQTLPTGSAIRIPPYSRIIGSSHLLNTSDEPVTTTMHLA
                     ITTIPPSQVVAKLAPARISYHDLHITPSAHSAFTTECDLASAYDGVWHRPLAYKLYYV
                     LSHYHSLGIYQQLEILGGPRDGEVIMRNDGFGDNAGHAITPPLDLAAAGAKGLRLTCG
                     YNNPRNVEVGWGIGDQEMCVMALQAVTDIGYDADVTRGTGASTGTSATGEVQNAGPCS
                     LTAFPWDFDKPGGKGH"
     gene            15277..>15669
                     /locus_tag="VMZ53_05610"
     CDS             15277..>15669
                     /locus_tag="VMZ53_05610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS27960.1"
                     /translation="MRAHAIVVGGGIFGVTAARSLRARGHEVTLIDPAPIGTPHPLAE
                     STDISKVVRMDYGADADYTELGERSLAAWRTEPLFHETGVMFISKVPLSGFERDSYDL
                     LTSRGHALQRLDANAIADRFPAWKRGTYV"
BASE COUNT         2606 a         5226 c         5076 g         2761 t
ORIGIN      
        1 ttcggcgaca aggccgacgc gaccgatgag ctgtgggagc ttcgcgcgag ctccgatcat
       61 tgggcgctgg tgtgcgggcc gacgcaaaaa ggctgtgcct gggaaccccg ctccgaagcc
      121 tcgcttgcct acctcgccgc gaccgactcc acgttcgcga tcggcggagc cggcgggaac
      181 ccgcgccacg aattcaaagg cgcgtggatc ctcgacgata acgagttcat tcattcgcac
      241 gacgatccgc ccgcgcgaga tttcgcgagc atgagctacg acccggagcg caagcgcatt
      301 gtcgtgtacg gcggcaatgg cgtcggctgc gccggcgatt gcgacggcac ctggatcctc
      361 gagtccgatt aacgcgccag gctcggcggc ggaaaccggc gcttgaacgt gaatgcgtgc
      421 tcggtcggtc cgtttgcctc gatcgcctcg agccgagctt tcgcctccgc cggcgtcggt
      481 agctcaccgc gagggatcca ccacatcgcg agcgccttcc cgccgacaac ggctttctcg
      541 tcgacgaacc agcgcttgcg ggccgcatag acctcggcgt gcgccgttcg atacgtgtac
      601 gtatgcagcg cctcgatcga ttcccacacg ctcagattga agagcacgcg cggatctgga
      661 aactcgcgct gcgcaggatc ccgagagtcg gtcagatatc gccacacgaa tccaggactc
      721 gcatccgcga gctgattcat ctcgtcgagc cgagcgatgt actccgcaat ccgcggatcg
      781 tcggcgtcac agagtgcgta ggcaacgttg gcctgcgcga gctgatacat ggcgccccag
      841 tgataactcg gctcgaggca cagacgcgat cgaaatgtga tgctcggacg ccctaggcga
      901 tcgcggcgta ggcctcggat agatcctgag cgcgaagtac gcgctgttgc atcgtcgcag
      961 acggggcgag cacgcggatg tgccgcttcg tcgagtgcac ggcgcctcgc tcaccgcggg
     1021 tcgcgatgct cgcatcgagg agtgcgcgat acaccgcgcg acgttcgtcg tcggtcggcg
     1081 gctcgatcgg ctcgccggcc agccgcgcgc gcgcctcgcg gaagatccac ggccggtcga
     1141 tcgccgcacg tcccaccatc acggccgcgc agcccgtctc ggcgagagcg gtaaccacgt
     1201 cgtccgcggt ccggacatcg ccgttgacga tcacgggaat gccgacgaca gcttgcgctc
     1261 gagccgccca gctccaatcc gcagggccgt tgtgccccat cgccgccgtg cggcaatgga
     1321 tcgtcagtgc ttcgatgccg gcatcttcga ggcgacgcgc gagatcgacg atcggcatat
     1381 gcgactcggg tccccagccg atgcgcgtct tcaccgtgac gggcagcgag acggtggccg
     1441 tgattcggcg aaccatctcc accattgcag caggatcgcg aagccagccg gcacccgcac
     1501 cgcgtcgcgc gatcttcggg atccaacatc cgcagttgat atcgatgaac gacggctccg
     1561 cagcttcggc gatccgcgcc gcagcgacga gcagatcggg atctgcgcca tagatctgga
     1621 tcgcggtcgg ccgatcatcg ggggccaacc gtgcgcgctt ttgcgcgagc ttagagccgg
     1681 cgatcagctg ctcgacgccg atgaactccg tcatgcagag cgaggctccg agcgagcgcg
     1741 cgacccgccg gaacgcggcg tccgtgacgt cctccatcgg cgcgagcacg acgggaaacc
     1801 cgtcgaggaa ttgcgtgatg cgcgagctcg ccacgacgct actgacagaa cgtcgtcgtc
     1861 gacggataga tcgtcatgcc cgcgaagcac atctcatcct tcgtgctctc gccaaacccg
     1921 actgcgctgc ccgtcgtgtt gttgtagctg cactcgatct tcagcacgtc gcctttatgc
     1981 agcgtgcgga tcggatcgaa catcacatgc tcctggccat cgaacgtgta gtcctggtcg
     2041 tagagcttgt acgcctcgcc cacggtcggt gtgaccgtat acgtgaggtg cgtaccgagc
     2101 tgatgcatgt gcggttgaaa cgagacgacg ttgatctcgg agagcgccgt gcacgtgccg
     2161 gtctgcgttg acacgcctgt cgggatattc agcgtgtcgg tcgggccgtt cagctccatg
     2221 cgcgcctggt tttgcacctt cgacgcttcg gtctctcgca cctcgacgcc ggacgtgccg
     2281 ctcagcacgg cgtctccggt gttgaagaga tgcaggttga tatggacctg ctgccccgcg
     2341 ctcaacttca gcgcgacacc ttccggatat tggaacggct tactccccac gccggtgccg
     2401 tagatgaagc gaccgccgaa ctcgaacggg ttgctgcact cgtacccggc gttatccggg
     2461 ccgcggaccg tgccgatcga catcacggtg tgatgcgttc cgatcgggat catcggccgc
     2521 agctcggtga tgtacatgtc gcgggggacc gtcacggtcg cgcactgata gagctcggta
     2581 cccggctgca gcgaccagct cgacgtgatc agcgtctgga acgagctacc gatcgctgcg
     2641 tcgggcgtcg gatccgttga ctcgccgcca cacgctgccg ccacgctcaa gacgagaagt
     2701 gctcgcatgc cgcgacgcta gcacgcgcga gcgctacttc cgctgcttcg ctacgctcgg
     2761 gtcggcaacg atcacatccg cgtggtcgtc ctcgggctcc ggcgcctgct gaccgagggg
     2821 gccagcaagc tcgggcggcg gtggagcggt aggggttggc gagcgcttgt cgacgggcgg
     2881 gagcccgtga tcgggtcgca gcaggtcgcc gagcatgatg ttctgctcga ggtcgccgat
     2941 cacgccatcg ggctccgtcg agctccaggt cgcgccggca gccgccttga cgatgcaggc
     3001 ctgcaacgct ttatcggtga gctcgccgtc gttcaccttc aggttctcga cgcggccaac
     3061 gccgtggttc gcgacctgtc ggtacgagag gcggatcgat tcgatcgtct cgtcgggacc
     3121 ggcgggccgg atcggcgtgg gcgccggacg accgcggtag cagcctttgg ccgcgtgctc
     3181 gatcgcgagg taacccggct cgagctcttc gggcagaccg tcgacgttcg cgtgctcgac
     3241 ggtgtcggcg tgctttacga cgccaggtgc tgcgagctga ccggtctcgt tcgggccggc
     3301 gctccgtgct tgcgcgcgac tgcggtcagc cgtgcgctcg gtgctgctgc tcgtcgtctg
     3361 cgcgttcgaa tcatccccat ggagcgcata gaacagcacc gcaccgagcg cgatcccacc
     3421 accgagtaat gcaagacggg acttcacggc agttcaccga aggacgatcg gcatcatcgt
     3481 gtagcccgac acgcgaagct ggcgcgggta gaacgtgccg tcgttgtagt tgtagaggta
     3541 gcccgcgcga ttcgtgtaca cgccgctgtt ctgctggttc gccgcgcaac caatcggttg
     3601 accgtagttg ttgtatacca tcgtgtagtg cgcgatctgg tgctgcgtac cgttgtagac
     3661 ggggcaggct gcgggatccg cggcgctcag gtccatgtcg tcgacgcggc acgtgccgat
     3721 cgagttcgcg ccggtcgtgg tccactccga ctcgaagtag tacgcggtcg agtccgggtc
     3781 cgggtcgttc acgacgggcg tgaagttgtc gtagacctcg atcgggcgcc ccgtgacggt
     3841 gtgaggcgtt ccgtcgccgc agtagtcagc cgtgaccatg cgcgtgcacg actggatgcg
     3901 cgtcgtcggg ctcgtgttac acgacgaggt gcccgtcatg tagttgtacg tgcagagcat
     3961 ggtgctgccg tagccgtagt agccgaggcc gcgcggcgct gcgttgctcg ccgagacggt
     4021 caacgtcgcc gacgacgtcg tcgaatgctc gccgtagacg gagatgtatg cgagataggc
     4081 ggagctcggc acggtcagcg tgcacgtctc ggtcaccgct tcgccgaggt tcgggcggca
     4141 gctgtacgtc gaggccgtgg cctggttgtt ccagcgaacg tacaggtttg gatcgccggt
     4201 gacgttcgac agcttcaccg tgatcttctc gccgggataa acgatccacg agcctttgtg
     4261 cgaccacgaa tacgccggca cggagaccga gccgctgtag ctcggcgtca gagggctcgt
     4321 cgtcgcttgg tcggaagtgc acttcgagat cgcgccgcct gcgcacgcga cggtgagcag
     4381 acttgccgag ccgctgatcc gcttgccacc gtcgatggtg ccttgcgtgt agttccacat
     4441 gccctgggtg aacgtcgccg gaaccggcgc cgtgcagttc gggttcgcgt tctcgcatcc
     4501 gcacagtggc gtcagcgctc cggtctgcgc gtccttccac ttcacgacgt gctggtagtg
     4561 cgtcgtccca tccgcgccgg cgatcgtctt gatcgccgtg atcttcaccg tcacgtatcc
     4621 ggcaagctga tcggtgtcgg cgtcgttgat cgtcgcttcg aacgttgcgt tgaggaattc
     4681 gtccttctcg atggtccggt agtgccacgc attcacgggc gcccagttcg gtccgaaatg
     4741 atcgaactcc cagacgttcg ccgagcccac gaggtagtcc ttcgtgacga cgacgttgtc
     4801 gatcggttgg ccctgcgcgg tcatcatcgg tccgaggttg aagcctccga tggagccgcc
     4861 gttcatgtag ccggcgttca tgtaaccagc gttcatgtag ccggcgttca tgtagccagc
     4921 gttcatgtag cccgcgttca tgtagccagc gttcatgtag ccagcgttca tgtagccagc
     4981 ggcccctgct gcggacggga gtgcgagggc cagaaagacc accgaagctc gacagacttt
     5041 ggagcgcatt ggccggccga tagagcaacg aatatgcctc gtcgcgacag gcgatcttca
     5101 gcgcgattcc agtcacttgc gatcgcggca acgcagtttt agcgtagctg ccttacgcgg
     5161 attctgcgta ctgcagtttc agcgcagcga ggcgcaaagt cgtacaccta atccgatgtc
     5221 gcgatagctc gcctcgacct ggttgtgctg ctcgattttt acggcgccga ggtcgaatcc
     5281 gaacgcaccg ccacggccac tcgcggtcac gccgggaact ccctggaggt tcgcggtcgt
     5341 catgtcgaag acgttgccga ccaatccgtc gagcccatac acggtgcgcg cgacttccgg
     5401 attctcgaga ttgatctcgt ccggaccgga gtcgaccgcg ccgccatacg tgaactgcca
     5461 attgccgttc tccggccgca cgtcatcgcc gatcgcatac atgcgaccgt ccgctccgcg
     5521 cgcggctgcc tcccactcgt cctcgctgca cagtcgcgcg ccgggaacgt atccggaacg
     5581 atcgagccat gctgcgtact cgatcgcgtc ttgctgcgag atgcccgaga ccggaagacg
     5641 tcgccagttc acgaccgcat tccgcgagcg acccgggtac gtgatgggct cgccatccca
     5701 cgcttcgagg cgagccgtcg cggatggctg caacgtcaaa cgccagcgac cgctgatgcg
     5761 ctcgagtcgt acgtaacctt ggacgccgaa cgtttgcaca cggggcgatc gcttctcgcg
     5821 ttcaccatcg ggcaacgtat cgagccatcg aatccactcg tcgaacgtga gctcggttct
     5881 cgagatgaag aatccgccaa cacgcatctc gtgacgcggc ggcgcatgct ggaaatcgcg
     5941 gcgatggcct tcctctgcgg tgctaccgat cagcacgcgc gccggtggga cgtatgcgaa
     6001 tccgtcggga actttgtccg gtagcgagac gacctcggtg acggtaacgc cgcgcccgag
     6061 gagcacggga tagtgcaccg gaggcctgcc agcgagcgcg aacgtgaacc ggtacgaacc
     6121 gtgcggaagg cccgcgtcat acggagcagg ttgcggctta ccaagtggtg cccactttcc
     6181 gtcgagccac cgctcgaccg cgatctgcgc gccggcgggc tggctatcga ggcgaacgtg
     6241 gcccggcgct gaccatgcgg cacgacgggt gccactgaca tcgtacaggt cgaggcgtgc
     6301 gagcaattcg acgagttgtt cgggccgctg atcgcgctcg gcgagcaagg cgcgatcgta
     6361 gatcacgtcg gcgagcacgc cgcgagcgtc tgcacggctt ggatcgatca tcagcgccgt
     6421 ctcgcccgct cggctcgcat caccgaacgc tcggtcggtc tttgtcgcga gcttgcgagc
     6481 ttctgcccac tgcttctcgc catcctcgta gtggtttgca tcgaagctcg cgaacgcttg
     6541 cacgcgcaca ttgccgaggt cggtcgcgag gcgacgcgct ttggcgagtg catcgcgcgc
     6601 aatgacgagc tcttcatcga ctcgacgagc acgccggttt tgttcactga gtcgtacgat
     6661 caggtagacg ctggcgagcg cgagcaccac gccgaccgct agcgaccagc gcacgatgcg
     6721 actgcgcgcg atcgtacggc gtgactggtc gacgaactcc tggtcgcgcg cactcaacgt
     6781 gtcgagcttc tcggcctcgg cgagctggcg tgtgctccac aagccttcgc gcgggcgacc
     6841 gagccgctgc cactctgcgg ctgcacgggc gaggcgttct ttgatcacgc ggcgatccgc
     6901 gtcgtcgtgg atccagccgc gtagcgtgcg ccactcctga atcagcgcct cgtgcgcgac
     6961 ttcgtatgcg gtgccatctg catcttctcg cgcgacgagc aggcgaccac gcacgagcgc
     7021 atcgacggcg gcgcgcgcgt tcgagtcatc accgacgagc tcggtctcgg cgacgcgtgc
     7081 gcgtgttcct tcggtcgtga cgagctgcag cagcacgcgt cgagcggccg cacggtgagc
     7141 ggggagcaag cgctcgagca caccgtcggc ttcacgcgtc agcgcacccg cgagaccacc
     7201 gcgtgcctcg agagactcag ccgtgatgac gtttgccttc gtatcgcgcg cgtcccagag
     7261 ctcggccagc acgaactgga gcagcggcag gtgcgtatcg gcggcttcgt cgatgagttg
     7321 ctcgatggtc tcttcgttct cgaagcgcac gcctttaatc tgggcgggac cgacgatcgc
     7381 ttcgcgcaca ccttcgggcg tcagcggacc gagcaagtag agggcgcgag tcacttcatc
     7441 gccgagcgcc ggtaggcgag caagccgcgt caggaagtcg ccgcgcacgg tgcagagtaa
     7501 gcgcacgccc ggcgacggaa tgttgaggcg cacgatcagc tcggcgacgg cagcggcttg
     7561 cgccggctcg gcgagcgtga cgagctcttc gagctgatcg atgaagatca agaggccgcg
     7621 gtcggccccc tggagcgcac gcagctcgcg cgcgagcgac gctggatcgc gcacgaggag
     7681 ctcggccagc gcaccctccc ctttgccgag catcggtgcg agcgcggcgg tgatcgtcgc
     7741 gaccggatgc ttcccgggca tgagcttgag cgttgtccac gtgcgatcgg gcccgaagcc
     7801 gccctcttcg acgagcggca acacgcccgc acggcacagc gacgacttgc cgacgccgga
     7861 gtcaccggcg acgaggatga acggctcgac gcgcaggcga tcgatgacgc cgcggatcgc
     7921 agcatcgcgt ccgaagaaga atgcgcgatg ctcggactcg aacgcggcga ggcctcgata
     7981 cggatttccc tcggggaccg ccttcgcgac ttggctcggc gcgagctgtt cgagcgcatc
     8041 gcgaagttgc tcggcagact cgtagcgctc ggctggatcg cggcgcaggc agcgatcgac
     8101 cacggctgcg aggcgcgagt cgacggtcgg cgccttgctg agcagcggcg gcgtctcgcg
     8161 cttttgcacc tcgaccatga gctgcggata cgggatgtcc ttgctcggcg gctggcccgc
     8221 gcacagctcg tagatcagcg cgccgagcga gtagacgtcg gagcgacgcg ttgcgacttc
     8281 gccacgccac acctcgggag ccatgtaata cggcgtgccc atcacggcgc cggcgcgcgt
     8341 gagcgacgga ttggaatcgg tcagcgacgg cgagctatcc ctcgcggtcg tcggcaccgg
     8401 cgtcgcgatc tgccgcgtgt cgtcggggtc gaacgccgct gccggtttcg ctgcgggcgg
     8461 aggcatcttc gcgccgaccg gcggaacggt gtcctcgtgc gagaccgagc gaccgttgcc
     8521 cgctgtcgtc ggaatcggcg tcgcgacacc gagtgggggc gtcgcatgtg cgggcagcac
     8581 ggtgtcgtcg tgaccaccgc ccgcgtcgac gatcacgaca ggcgagcgag cgcgctttgc
     8641 gggctccggc ggcggctcct cggggacggc agcgtcgatg agctttgcga gcccgaaatc
     8701 gaggagcttg atgtcgccgt tctcggtgag cacggtgttc gcaggcttga tgtcgcgatg
     8761 caggacgccg cgccggtgag ccgccgcgag cccacgtgcg agcccgatcg cgagctcgag
     8821 tgcgcgctgc cagggcatcg gtcgcggtac tcgatcgagg cgctcgccgc gcacgaattc
     8881 cgagatgagg taaggccgct gttcgagctc gccgacgcga tagaccgcga cgacgtttgg
     8941 gtgctggagt cgcgcggcgg cacgcgcctc gacgaagaac cgctcgcggg cgccaacgtc
     9001 ggcctcgaga tcggcggcga tgaacttcac cgcgacaggt cgatcgagca gccgatcgtg
     9061 cgcgaggaag acatcgccca tcgcgccggc gccgaggtgc ttgacgagct tgtactcgtc
     9121 gaactcctgc tgcggtggcg tccagcggcg cgccatcgct agccccgact cttctggccc
     9181 gggcgaggga tgtcgtagaa gcgaagcttg gcctggaacg ttcgcagcgg catcgcgatc
     9241 aagtcggccg cgcgcgtctg attgccaccg gcagcggcga gcgcctcgat catgcgccgg
     9301 cgttcgagat ccttgatctc gtcttcgatc ggccggaact tcgtcggcga cacgctgtcg
     9361 gaaccaacga tgggaatcga cagcggcgtg ttcgtgcgac cgcgcttcgg cgctgctgct
     9421 tggagctctt cgtcctcgtc gccttcggac ggtcccgaca gcgaccgcga caagtgccac
     9481 gcctgcagca cgggctcggt gaacgctgcg gcgacgtagt ccatcacgtt cttgagctcg
     9541 cgtacgttgc cgggccactc gtacgtcgcg agccgttgca tcgcgtcgtc ggagatttcc
     9601 atcgtcgcgc ggttcgcctg cttgcacgcc tcggcgagga acgtctgcgc caggatcgcg
     9661 agctcgcggc ggcgatcgcg cagcggcggc agccacagcg tcgcggcgct gagccggaag
     9721 aacagatcct ggcgaaagcg gccggccttg acctcatcgg cgaggttgcg attcgtcgcc
     9781 gcgacgagcc ggatatcgat ctcgcgctcg cgcacatctc cgacgcggat cacgcgcttc
     9841 gtctcgagca cgcgcaggag ctttgcctgc gtcgtcggcg acatctcgcc aatctcgtcg
     9901 aggaacaccg tgctgcccga agccacctcg agcaagccgg acttcgcagc taccgcgccg
     9961 gagaacgcgc ccttctcgtg accgaacagc tcgctctcga cgaggttctc ggacagcgcg
    10021 gcgcagttga acgcaacgag cgacttcgcc ttgcgcgccg accactggtg caccgcgcgc
    10081 gccgcgagct ccttgcccgt cccggtctcg ccgcagatca gcacgggcag ctccgcggcc
    10141 gccaagcgct cgatcaatgc atacagccgc gacatcgccg gatccgcgac gatcacggtg
    10201 tgatcggcga tgcgcttcgt ctcgtacgtg cgcgccgctg caccaacatt gcccgcgtcc
    10261 gccgcgagcg ccgcttcacg cgcgctcgaa acgagcgtct cgacatcgca gccgtcgtgc
    10321 gggcacgtcg caaagccgac cttcgcgcca ggcgcaatcg gcaccagcga cttcagcacg
    10381 cgtgccgccg cctgcggcgc tagatcgacg tcgcactccg gcatcaagat gatcagctga
    10441 tccgacgcgc cgctgccgac gacatcgaga atgcgcagtg acgcgcccag cgcatgcgcg
    10501 acgcgagggc ggtgactcgg ctcgatctgc agaatcgcga cgagtgtcgt gagtggccgg
    10561 tgatagtgca acgcgcgctc gatctcctcc tcggtgcgcc gccgcatctc ctcgaacttc
    10621 aggatcggcc ggtgcacgcc cgccggcatg cggacgctcg cgtgaaagac gatcgagatc
    10681 gagcaaatcg agatcgtgtc accggtaaag agccgccgcg ttccggtgat gcgctcgcca
    10741 ttcacgagcg tgccgttctg gctctcgagg tcgcagatgc gcgcttcgcc cgccgccatg
    10801 gtgacctgcg cgtgcatgcg cgagacggac gtatcgctca accgcagcgt cgcgccctcg
    10861 ccgcggccga tcgtgacgtc acccgcggcc ggcaggctga acatcgaaga cgacgcgccc
    10921 tcgaagacaa gcaagtacgc ctgatcgccc agcggctgcc ccgcatcgcc gtgcggctgg
    10981 ccaatgctcg cgcccgatgg agcgatcgtt gactcgtccc aatcagccgg agtcttccgg
    11041 ccgcggtcgg tgctgtcccc cgaggacgta ctcattgcgg acccgaccaa gatctcaccg
    11101 caaacccgac gggacaagcc ggttaggcgt cgacaaaccg catccggaga cgccgagagg
    11161 cggtggctcg tcgaggttga cagcacacgg cgattcgggt cgcgatcagg cttcctagcc
    11221 tcacgtcttc gcgatggtca gcggcgcgcc gtctatcgtg accgtgccag atgtcgtccg
    11281 gccgacgatc gagcgcgcga cgacgcgatg cgagaccgct tcgtggacca gcgtcgcagt
    11341 cagcgtgatg tcgtgctgca tcacgagtgg ctcgctcgcg acctcgacgc catcgatcac
    11401 gagcttgagc ttcttggcga ggttgtcccg aacgagctcg atcctgtgcc cctgatacgt
    11461 gccgcggtat ccgtcgccca tcagtcccat gcgtgcaccg ctacggtttg aactggtcgg
    11521 caccgatatc gggcgggctg acacgcatgt cgccatcaat atcgagggcg gcgatgccgg
    11581 tgagatcgga ggcggggtca ccagctccgc gcgccgggct gcccgccttc aaatggaggt
    11641 tgccggcggc atcgtcaacg aacatcggat ccgtggcgat gttgccagtg cccgtcggaa
    11701 ccgcacccgg ccgtgagatc gagtaggcgt gcaggcaggt gccagagaac tggttcgcac
    11761 cgcccatgtt gccactcatg atgttgctgc gcgccgtgaa ggtaccggcc gtgcactgga
    11821 tcgcagcggc gttcgacgtg ctggtccggt tccccacgaa cgtgttgagc tccagccggt
    11881 ttccggcgct ctgagcggtg ttgatcgtga cgccgcccgt cgtgccggag gtctgcccgt
    11941 taccgaaaaa cacgtttccg acgatcgaga acgtaccggt cggtgacacc gagatgccgc
    12001 cgttgatgtt gttcacgaat gtgctgcggc tgaccgacag cgagccggcg tagtagatga
    12061 tggcttcgcc gccgctgttg ccggacagtg tgctcgcggt gacggagacg acgctgttgt
    12121 ggctgaagat gccgccggcg ccggtgttgt tcgcgatcgt cgagcgcgtg actgtgagcg
    12181 tgccgctctc catcacgatc ccaccgttcg cgttgttctg gaccttcgcg cgcgacagga
    12241 acaacgacac ggtcgtgccg gtcggcacgt tgatgcccgt gaccgaattc gagatcgtca
    12301 gatcatagat ctcgacgtgg ctcgagcctc cgagcgtgag gccgttgttg ccgctgttct
    12361 tcaggatcgc gcccggatcg gcaaggaacg tcacgttacg gttcgtgagc gagacgctct
    12421 cgtcgatcgt gccatgcacc ttgatgtacg ggcggttcgt cgcgagcgcc gccgagatct
    12481 gcgggcacgg catcgcgcgc gtgcactgcg tgctgcccac ggcagatgca tcgacgaatg
    12541 cgacgttggt gtccgtaccg cacgagccgt cgggaaggca tgcgttcgag gcgcagtcgg
    12601 agtgagcctc gcacgcacga caggtgttgt cggtcgcgca gacgggcgtc gtaccggcgc
    12661 aagccccgat ctcggacgcc gtgcacgcga cgcagaccat gctcgacgtg ttgcatacgg
    12721 gcgtcgcgcc actgcactgc gcggtcgtcg tgcagcccat ctgaccatct ccacccgagc
    12781 ccccggcgtc tgtcttctgt cctgtcacct gaccgcatcc gaagacgaac gcgacgacga
    12841 cgacgacgca caactgcgca gcataggtct tcaacatcgg gccgaatcat atgaccaaac
    12901 caagagcagc tggctgacga gcagagcgtt tcgcagggga ttcgcggaca cgatttcggt
    12961 gaagcatcgg cgcacatgtg cgtctcttca ctcgaacacg gagcgtgttg cgctcccaac
    13021 gaagggtgaa tgtatgaaac gccgtctact gatttctcta atcgctgctg gtgccgcact
    13081 gggctccggc gttgcttctg ccaatgcgtc ttactgggcc caagaaggcc ttctcctcga
    13141 ggtcagcgcc gatccgcgcg acgggtcgga cttcgtgcag acgaatccgg tccacgcgca
    13201 gagcctcgag ctcgtcgcgc tcaatgacaa cgttcgcctg cgtggcatga ctgtccggtt
    13261 tacggacggc cgcacgttct cgcaggacat gcgaacgatt cgcgcgggcg agcacgtccg
    13321 catcgatctg ccggagaact gcggcacgat ccagggagtc cagctcgact acggtcaaca
    13381 gctcgtcgac cgcacgaccg ctcgcctcca gatcatcccg cgcgaagatc gtcagatcgc
    13441 ggcactgccg tcgcggcccg tgatccgcga ccaccgctgg agccggcacg agtaccgcta
    13501 cattgcgccg cagccggcgt atgtcgcgcc gcagccgacg tacgtcgcgc cgcagccgac
    13561 gtattcgtac caacagccga cgtacacgta tcagccgagc tcgtatcaca cgacgccgac
    13621 gtacgtggtg ccggcgccgc gtccgcagag cagcatcggc atctcgggct acatccaagg
    13681 gagcttccgc ttctaagcaa acgctgacgt agtcctcctc caaaccagct ccggtttcgc
    13741 gcgcgacgcg gagccggagt ttttcgtttc ggttcgacta ctggccgccg cccatttgcc
    13801 cgccgcccat ctggccgccg cccatctggc cgcagcacat gccgccgccc atctgctgca
    13861 tttgctgctg cggtgcctgc tgctgctcgc cgccgcccgg ctgctgctgt tggccgccgc
    13921 cttggaacgc gccgatcatt ccttgaatgc tctgccagat gccgctgatg ccttgcgtga
    13981 tcgcctgacc cttgggcccg aggaagctac cgatgccttg catgagcgcg ccaatatcga
    14041 agccgccgcc aacgcgctcg agctctcccg cgctgatcgt ttcgaacgag tccttgtgca
    14101 tgcgcccgca gaagtgcagc caccgtgccg cgattcggag ctgtagattc cgctagttgc
    14161 cacgcgcacg ctgtctaccg cgagtggcgg gatgagccct cgccgtggta gcccacgtcg
    14221 atgaagcgcg cgctggtctc ggtcgggttg gtgctcacgg gctgcggcaa cgatccatgc
    14281 gatcccgcgg cggagtcgtg cacctacgaa gcggacgtca gcacgctgac gatcggcggg
    14341 ggcgtcgaag atgaagacac ctgccagagc tggacgctga acaatccaac ggagctctgg
    14401 gtgacgtcga tcgcgcaatc gaatgacggc gcgtatcacc acgcgaactg gttcttcgtt
    14461 cccgacgatc agtacaagct gcccgacggc acttgggcgt gcggcgcgaa ccaattcgaa
    14521 gagctcgtcg cggcactgca aggcggttac ctcttcgcgc tcagcacgca atcgcacaac
    14581 gagcagcaga cgctccccac aggcagcgcg atccgcatcc cgccctacag ccgcatcatc
    14641 ggctcgagtc acttgctcaa cacgagcgac gagcccgtca caacgacgat gcacctcgcg
    14701 atcacgacga tcccgccatc gcaggtcgtc gcgaagctcg cgcccgcgcg catctcgtat
    14761 cacgaccttc acatcacccc gtcggcgcac agcgcattca cgaccgaatg cgatctcgcc
    14821 agcgcgtacg acggcgtctg gcatcgcccg ctcgcgtaca agctctacta cgtgctctcg
    14881 cactaccact cgctcggcat ctaccagcag ctcgagattt tgggcggccc gcgcgacggc
    14941 gaggtcatca tgcgcaacga cggtttcggc gacaacgcag gtcacgcgat cacgccgccg
    15001 ctcgatctcg ctgccgcggg cgccaaaggc cttcgcctca cgtgcggcta caacaacccg
    15061 cgcaacgtcg aagtcggttg gggcatcggc gatcaagaga tgtgcgtcat ggcactgcaa
    15121 gcggtcaccg acatcggcta cgacgccgac gtcacccgcg gcaccggcgc atcgacgggc
    15181 acgtcggcaa ccggcgaagt ccaaaacgct ggcccctgct cgctcaccgc gttcccgtgg
    15241 gacttcgaca agccgggcgg caaaggccac tgacacatgc gcgcgcacgc gatcgtcgtc
    15301 ggtggcggca tcttcggcgt caccgccgct cgctctctcc gcgcgcgtgg ccacgaagtc
    15361 acgctgatcg atccagcgcc gatcggcacg ccacatccgc ttgccgaatc gacagacatc
    15421 tcgaaagtcg tccgcatgga ctacggcgcc gacgccgact acacggagct cggcgaacgc
    15481 tcgctcgctg catggcgcac ggaaccgctc tttcacgaga cgggcgtcat gttcatctcg
    15541 aaggttccgc tctcaggctt cgagcgcgac agctacgacc tcctcacatc acgcggtcac
    15601 gccctgcaac gcctcgacgc aaacgcgatc gccgatcgct tcccggcgtg gaagcgtggc
    15661 acgtacgtc
//
LOCUS       DATLBZ010000107         6641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4570160, whole genome shotgun sequence.
ACCESSION   DATLBZ010000107 DATLBZ010000000
VERSION     DATLBZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4570160"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VMZ53_05615"
     CDS             complement(<1..797)
                     /locus_tag="VMZ53_05615"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27961.1"
                     /translation="MSTASSAQPKQFDLPSGTMLGKYEILKKLATGGMAEIYLARVRG
                     NAGFEKLVVIKRILPTVADDPTFVRMFLDEARLAATLHHPNIADVYEVGEHEGAPFFA
                     MEFVHGQDVRAIRQGARKTKERVPLAIALAIIHGGASALDYAHDRTGSDGKPLNLVHR
                     DVSSSNILVSYEGAIKLIDFGIARATSRTHQTQVGTLKGKIPYMSPEQCRGLPLDRRS
                     DLFSLGVVMYELTVGRRPFRGETDFGIMEQIVHGEAPAPSTLVDDYPP"
     gene            828..1556
                     /locus_tag="VMZ53_05620"
     CDS             828..1556
                     /locus_tag="VMZ53_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27962.1"
                     /translation="MTKTHTAAPLALCALLFACNDKPATPTAKGPHVEIFTDENARTV
                     AVGEPVALASLVAPPPGSWLEVRADTADARFLELAPRQNDEIRLYIDHDKVAIGVFPP
                     VTPDMSPEVRARANQPFSSLVGVTTVRVATHLPSLPNLTVAVGGKDIVLSDALRGLAA
                     TGKRRAQGWPLEDVLTLAGAANATSARIVGAQEITLDRSEIVKALIKPNQRGEYVIRV
                     WDDGAKAPTREVRSVTKIVIGDPD"
     gene            1586..3619
                     /locus_tag="VMZ53_05625"
     CDS             1586..3619
                     /locus_tag="VMZ53_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HUS27963.1"
                     /translation="MLRGLLVISTLLLLLDGCSGGECGDGTFRYGDTCVAVDPFDKTP
                     PKVAVDPPLYTRAVGIVRLTADEPSTIYYTIDGTSPTTDSSHEDDVVVVANVPDNAQL
                     RYFAVDRNGNRSAEELRIWIIDRNGPGAPIDFRLALSGSSRSLTWVTPPDPRLGGVMI
                     ARVDGPLAAGPESGTAYAVGDTISPGVQVVSLNGTDTAGAFNESVATSPGLVRYIAWA
                     FDDLHNYGPPAGDYAVVPMAAQSAAVSVTANTNATFSITTPPSLLNLTGDAQYTSGNQ
                     TLTVHLTLRNDTARVLYAPKLVLKTALPGTVAFNKDGDYNAKPYRAYGAALLPAQTTQ
                     QTWTFTNVTSGTTLNLGLDFVDGQIAGTSAYRSDSAGQIVDFNTGVQLLQLAALPMGN
                     GGDGMMIRGGFTPDGKLICGSRTAGAVSRYDLVSGSRELTAELKPQKAHVPAILLDKS
                     GSAVYALLAEGHPYNINNNSATAGATTELVMLDAGSLQIGARLPIGPSRNRGIEISPD
                     GRLMLIATGLQAQGVIVVDLTQLTIKTRIIPPFRPQVALFAPDGSIVIVGEQVAVYTQ
                     EGNRTALWETPGTGGKVVRAAFGSPTLLWIGRRDQLEKLDIRDGVGSTVTLPPGNPTP
                     PTLGGRIVEVFDGKLYAMAGSDIVRLDANATTIEKTLDGFDDVDAHWFGRSPF"
     gene            3619..5667
                     /locus_tag="VMZ53_05630"
     CDS             3619..5667
                     /locus_tag="VMZ53_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HUS27964.1"
                     /translation="MRRLLALALLASASSACSDVECGPGTFSNGNGCIGYDPNDKDPP
                     VVLLSPAGGRSREALPSLVEMSTNEEATIYVTTDGSDPDPMTTMGETTPVKIVGITQG
                     ETLKYLAVDRAGNRSTIGSTTFESDTTAPAPVSSISITMNGTTPHVTWTPPTDADYAG
                     TVVARVVDLIDVSPPVGEMVTNGAALSASLKVVSVGTGTSFDDTALPPGPTRYVAWTY
                     DDLGNYSTGKAASVTIPIGALTAQLTFNTANNTLAVVTPPANFSLAGTTATNNAGTVT
                     VSLQIKNNTNQYFLNPKIEIVSTTNGTFTQSDGNADGRPFKSLGPDVFAPGATKTASL
                     IYTGATGTVTMNLTFATHPTLTVHSSRRQGMTTFDLGSGNALPEFTTSARGPQDRQGG
                     AVRFPLLTGGRFLDFPTSHGTIERYDLVNHTKAGQINLGTGDRTNIQWMISTRGEVLA
                     MVKNGGKRRIGTIELVRLDEGMHELGRVKLPYSEDRGFVKPAISPDGNTLAFPVVGGI
                     LLLDVATLKPIDPIPATSAIDLITTGFTNTRVKALTFFNGTDGIVAIARNNGQAVIIK
                     RANGDYTKTTYQDANATVRGFGVALAPDGKVWFSFDEPNGIRVYDPATDGISNLAYAT
                     QPQGIANVEGQMWIIRTDKLNLDQVNNAGAVQRTITIPNPSGLTYTAGAMGHTLDSIR
                     "
     gene            5708..>6641
                     /locus_tag="VMZ53_05635"
     CDS             5708..>6641
                     /locus_tag="VMZ53_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUS27965.1"
                     /translation="MTIALGIDLGTTNSCAAVATPTGVEFILGQRAERVHPSVVAFPN
                     TGGVVVGADAKRFRLTEPQNVIHSAKRFIGQNIRAPLVQLALTGIRYKVIEGPNQQPI
                     VVVRDRRMTMPEISSHVLLHLKRCAERQLGESVTDAVVTVPANFTDAQRQATKEAGRL
                     AGLNVMRLINEPTAAALAYGFGRNQDQLVCVFDFGGGTFDVSILRIHGEVFEVLASDG
                     DYFLGGDDLDRALAEVLAAECNRSLGFDPRQHPVLMMKLMMGAEAIKCHLSDNDAAEG
                     EIDGIDLPNGATGSLPFALTRAQFEHLIAGYVNRT"
BASE COUNT         1235 a         2259 c         2057 g         1090 t
ORIGIN      
        1 ggcggatagt cgtcgacgag cgtcgacggc gccggtgcct cgccgtggac gatctgctcc
       61 atgatcccga agtcggtctc gccgcgaaac ggccgccgcc cgacggtgag ctcgtacatc
      121 acgacgccga gcgagaacag atcgcttcgt cgatcgagcg gcaacccgcg gcactgctcg
      181 ggcgacatgt acgggatctt tcccttcagc gtgccgacct gcgtttgatg cgtccggctc
      241 gtcgcgcgtg cgatgccgaa gtcgatcagc ttgatcgcgc cttcgtagct gacgaggatg
      301 ttgctcgacg acacgtcgcg atggacgagg ttgagcggct tgccatccga gcccgtgcgg
      361 tcgtgtgcat agtcgagcgc cgatgcgccg ccatgaatga tcgcgagagc gatcgcgagc
      421 gggacgcgct ccttcgtctt gcgcgcacct tgccggatcg cacgcacgtc ttggccgtgc
      481 acgaactcca tcgcgaagaa tggcgcgccc tcgtgctcgc cgacctcgta cacatcggcg
      541 atgttcgggt ggtgcagcgt cgcagcgagc cgcgcctcgt cgaggaacat gcgcacgaac
      601 gtcggatcgt cggcgaccgt cggcagaatg cgtttgatga cgacgagctt ctcgaagccc
      661 gcgttaccgc ggacacgcgc gagatagatc tcggccatgc cgcccgttgc gagcttcttg
      721 agaatttcgt acttgccgag catcgttccg ctcggcaggt cgaactgctt cggctgcgcg
      781 gacgaagcgg tagacacgcg aagacaacgg taccatcgac gggccgcgtg acgaaaaccc
      841 acaccgcagc acctctggct ctctgcgcgt tgctcttcgc gtgcaacgac aaacctgcga
      901 cgcctacggc gaaggggccg cacgtcgaga tatttaccga cgagaacgcc cgcaccgtcg
      961 ctgttgggga acctgtcgca ctggcatcgc tggtcgctcc tccgccgggg agctggctcg
     1021 aagtccgcgc cgatacggca gatgctcgct tcctcgagct cgcgccgcgc cagaacgacg
     1081 agatccggct ctatatcgac cacgataagg tcgcgatcgg cgtgttcccg cccgtgacgc
     1141 ccgacatgtc acccgaggtt cgcgcgcgcg cgaatcagcc gttctcctca ctcgtgggcg
     1201 tgacgaccgt gcgcgtcgcg acgcatctgc cgtcgctgcc gaacctgacc gtcgccgtcg
     1261 gtggcaagga catcgtgctc agcgatgcgc ttcgcgggct cgcagcgaca ggcaaacgac
     1321 gcgcgcaagg ctggccgctc gaagatgtgc tgacgctcgc aggagcagcg aacgcgacga
     1381 gcgctcgcat cgtcggtgcg caagagatca cgctcgaccg cagcgagatc gtgaaagctc
     1441 tcatcaagcc gaatcagcga ggcgagtacg tgatccgcgt gtgggacgat ggagccaaag
     1501 caccgacgcg cgaggtacgc agcgtgacca agatcgtcat cggcgatcct gactaatcga
     1561 aacaggcgtg agagcctgta aatgcatgtt gcggggcttg ctcgtcattt cgacgctgct
     1621 tctgcttttg gacggatgca gtggcggcga gtgcggcgat ggaacgtttc gctacggcga
     1681 tacgtgcgtt gccgtggatc cgttcgacaa gacgccgcca aaggtcgccg tcgatccgcc
     1741 gctctacacg cgcgctgtcg gcatcgttcg cctcacagcc gacgagccat cgacgatcta
     1801 ctacacgatc gacgggacat cgccgaccac ggatagcagc cacgaagatg acgttgtcgt
     1861 cgtcgcaaac gttccggaca acgcgcagct gcgttacttc gccgtcgatc gcaacggcaa
     1921 tcgcagcgcg gaagagctac gcatctggat catcgatcgc aatgggcccg gagcgccgat
     1981 cgatttccgg ctcgcactgt ccggctcgtc gcgcagcctc acgtgggtga cgccgcccga
     2041 tccgcgcctc ggcggcgtga tgatcgcgcg cgtcgacgga ccgctcgctg cagggcccga
     2101 gagcggtact gcatatgccg tgggcgacac gatctcgccg ggcgttcagg tcgtctcgct
     2161 gaacggcacc gatacggcgg gcgcattcaa cgaatcggtt gcgacatcgc ccgggctcgt
     2221 gcgctacatc gcgtgggcgt tcgacgatct gcacaactac ggtccgccgg cgggtgacta
     2281 cgccgtggtg ccgatggcag cgcagagcgc ggcggtatcg gtgacggcga acacgaacgc
     2341 gacgttctcg atcacgacgc caccctcgct gctcaacctc accggcgatg cgcagtacac
     2401 gagtggcaac caaacgctca ccgtgcacct cacgcttcgc aacgacaccg cgcgcgtgct
     2461 ctacgcaccg aagctggtgc tgaagaccgc cctgcccggc accgtcgcgt tcaacaaaga
     2521 tggcgattac aacgccaagc cgtatcgcgc ctatggcgcg gcgctacttc cagcgcagac
     2581 gacgcagcaa acgtggacgt tcacgaacgt gacgagcggc acgacgctga acctcggcct
     2641 cgacttcgtc gacggacaaa tcgccggcac gtccgcgtat cgctccgaca gcgccggcca
     2701 gatcgtcgac ttcaacacag gcgtgcagct ccttcagctc gcagcgctgc cgatgggcaa
     2761 cggcggcgac ggcatgatga ttcgcggcgg cttcacgccc gatggcaagc tcatctgcgg
     2821 ctctcgtacc gcaggtgcgg tgtctcgcta cgatctcgtg agcggcagcc gcgagctcac
     2881 tgccgagctc aagccgcaga aagcgcacgt gcccgcgatc ctcctcgaca agagcggatc
     2941 ggctgtctac gcgctgctcg ccgaaggcca cccgtacaac atcaacaaca acagcgcgac
     3001 cgcgggcgca acgaccgagc tcgtcatgct cgacgctggc tcgctgcaaa tcggcgctcg
     3061 gcttccgatc gggccgagcc gcaatcgcgg gatcgaaatt tctcccgacg gccgcttgat
     3121 gctgatcgcg acgggcctgc aagcgcaggg tgtcatcgtc gtcgatctca cgcagctgac
     3181 gatcaagacc cgcatcatcc cgcccttccg gccgcaggtc gcactgttcg cgcccgacgg
     3241 ctcgatcgtg atcgtcggcg agcaagtcgc ggtctacacg caagaaggca atcgcactgc
     3301 gctgtgggag acgcccggca ccggcggcaa ggtcgtgcgc gctgcgttcg gttcaccgac
     3361 gctcttgtgg atcggccgtc gcgaccagct cgagaagctc gacatccgcg acggggtcgg
     3421 ctcgaccgtg acgctgccgc ctggcaatcc gacgccgcct accctcggcg gccgcatcgt
     3481 cgaagtgttc gacggcaagc tctacgcgat ggccggcagc gacatcgtgc gcctcgatgc
     3541 gaatgcgacc acgatcgaga agacgctcga tggtttcgat gacgtcgacg cgcactggtt
     3601 tggaaggagc ccgttctgat gcgtcgcttg ctcgcgctag cgctgctcgc atccgcgtcg
     3661 tctgcctgct ccgacgtcga gtgcgggccg ggaacgttct cgaatggcaa cggctgcatc
     3721 ggctacgacc cgaacgacaa agacccgccc gtcgttttgc tttcacccgc cggtggccgc
     3781 agccgtgagg cgctgccttc gcttgtcgag atgtcgacga acgaggaagc gacgatctac
     3841 gtcacgaccg atggcagcga ccccgatccg atgacgacga tgggcgagac gacgccggtg
     3901 aagatcgtcg gcattaccca aggcgagacg ctcaagtatc tcgcggtcga tcgtgcaggc
     3961 aatcgctcga cgatcggcag cacgacgttc gagtcggaca cgacggcgcc ggcgcctgtc
     4021 tcgtccatct cgatcacgat gaacgggacg acgcctcacg tcacgtggac gccgccgacc
     4081 gacgcagact acgcgggcac ggtcgtcgca cgcgtggtcg acctcatcga cgtctctccg
     4141 ccggtgggcg aaatggtgac gaacggcgcg gcgctctcgg cgtcgctgaa ggtcgtcagc
     4201 gtcggtaccg gcacgtcgtt cgacgacacg gcgctgccgc ctggaccgac tcgctacgtc
     4261 gcgtggacgt acgacgacct cggcaactac tcgacgggca aagccgcgag cgtgacgatc
     4321 ccgattggcg cgctgaccgc gcagctcacg ttcaacacgg ccaacaacac gctcgcggtc
     4381 gtgacaccgc cggcgaactt ctcgctcgca ggtaccaccg cgacgaacaa cgcgggcacc
     4441 gtcacggtaa gcctgcagat caagaacaac acgaaccagt acttcttgaa tccaaagatc
     4501 gagattgtct cgacgacgaa cggcaccttc acgcagtccg acggcaacgc cgatggccga
     4561 ccattcaagt cactcggccc cgatgtgttc gcgcccggcg ccacgaagac cgcttcgctc
     4621 atctacaccg gcgcgaccgg caccgtgacg atgaacctga cgttcgcaac gcacccgacg
     4681 ctgaccgtgc actcgtcgcg tcgccagggc atgacgacgt tcgacctcgg ctctggcaat
     4741 gcgctccccg agttcacgac ctccgcgcgt ggcccacaag atcgccaggg cggcgcggtg
     4801 cggtttccgc tgctcaccgg cggtcgattc ctcgacttcc cgacatcgca cggcaccatc
     4861 gagcgctacg acctcgtgaa tcacacgaag gcggggcaga tcaacctcgg caccggcgac
     4921 cgcacgaaca tccagtggat gatttcgacg cgtggtgagg tgctcgcgat ggtcaagaac
     4981 ggcggcaagc ggcgcatcgg taccatcgag ctcgttcgcc tcgacgaagg catgcacgag
     5041 ctcggccgcg tgaagttgcc ctacagcgag gaccgcggct tcgtgaagcc cgccatctcg
     5101 cccgacggaa acacgctggc gttccccgtc gtcggcggca tcctgctcct cgacgtcgcg
     5161 acgctaaagc cgatcgaccc gattccggcg acgtccgcga tcgacctcat cacgaccggg
     5221 ttcacgaaca cgcgagtcaa ggcactgacg ttcttcaacg gcaccgacgg catcgtcgcg
     5281 atcgcacgca acaacggcca ggccgtcatc atcaagcgcg cgaacggcga ctacacgaag
     5341 acgacctatc aggacgcaaa tgccaccgtc cgcggcttcg gcgtcgccct ggcccccgac
     5401 ggcaaggtct ggttctcgtt cgacgagccc aatggtatcc gcgtctacga tcccgcgacg
     5461 gacggcatct cgaacctcgc ctacgcaaca cagccgcagg gcatcgccaa cgtcgagggc
     5521 cagatgtgga tcatccgcac cgacaagctc aacctcgacc aggtcaacaa tgcaggcgcg
     5581 gtccagcgga ccatcacgat tccgaacccg agcggcctga cctatacggc cggcgcgatg
     5641 ggccacacgc tcgatagcat ccggtaaatg gcaacccgcc ggaacctgcg ataatttcga
     5701 gagctggatg acgatcgcgt taggtatcga cctggggacc acgaactcct gcgccgccgt
     5761 tgcgactcca acgggcgtcg agttcatcct gggccagcgc gccgagcgcg ttcatccctc
     5821 ggtcgtcgcg ttcccgaata cgggcggcgt cgtcgtaggt gctgatgcga agcgcttccg
     5881 cctgacggag ccgcagaacg tcatccactc cgcgaagcgg ttcatcggtc agaacatccg
     5941 cgcaccgctg gtgcagctcg cgctcaccgg cattcgctac aaggtgatcg aaggcccgaa
     6001 ccagcagccg atcgtcgtcg tgcgcgatcg ccgcatgacc atgcccgaga tcagctcgca
     6061 cgtgctgctg catctcaaac gctgcgccga acgccagctc ggtgaatcgg tcaccgacgc
     6121 cgtcgtcacg gtgccggcga acttcaccga cgcgcaacgc caagcgacga aagaagccgg
     6181 tcgcctcgcc ggcctcaacg tgatgcgcct gatcaacgag ccgacggcgg ccgcgctcgc
     6241 gtacggtttc ggccgcaatc aagatcagct cgtctgcgtg ttcgacttcg gtggcggcac
     6301 gttcgacgtc tcgatcctgc gcatccacgg cgaggtcttc gaagtactcg cctccgatgg
     6361 tgattacttc ctcggtggcg acgatctaga tcgcgcgctc gccgaagtgc tcgccgccga
     6421 gtgcaatcgc tcgctcggct tcgatccgcg tcagcacccg gtgctgatga tgaagctcat
     6481 gatgggcgcg gaggcgatca agtgtcacct ctctgataac gacgctgccg aaggcgagat
     6541 cgatggcatc gatctgccga atggcgccac cggctcgctg ccgttcgcgc tgacgcgcgc
     6601 gcagttcgag cacctgatcg cgggctacgt gaaccgcacg a
//
LOCUS       DATLBZ010000108        12220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_848558, whole genome shotgun sequence.
ACCESSION   DATLBZ010000108 DATLBZ010000000
VERSION     DATLBZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848558"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VMZ53_05640"
     CDS             complement(<1..1153)
                     /locus_tag="VMZ53_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS27966.1"
                     /translation="MGTAAGHHDEEARSYLQARLCVLSVVLAWGFGGFMLLVALIYSR
                     YPEIEPPAAGSLNRAAFAVVAVLAAGRVFLRSPRSMRVLHSVDAAYAACFGVWLACSA
                     IYSWQLHDAGYTALLLGIFIVFTRAIILPSSARRTAILSSLLLGCLVYSSIVLALTVE
                     QDLPPLPFVIGAVVLSSVATVLAALGSSILHGLRRNFDKAVKLGRYTLDAKIGEGGMG
                     VVYRAHHVLLRRPTAIKLLQKGRIGADALRRFEREVQHTSQLTHPNTVAVFDYGHSPD
                     GIFYYAMEYLDGVDLEKLVRTSGPQRPGRVIRILEQVCGALQEAHDRGVIHRDIKPAN
                     IILCERGAVPDVAKVVDFGLAKEIAADGDTTQVILGTPAFLAPEAITAPS"
     gene            complement(1159..2388)
                     /locus_tag="VMZ53_05645"
     CDS             complement(1159..2388)
                     /locus_tag="VMZ53_05645"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HUS27967.1"
                     /translation="MRWLVLVSLGALGCSAFHSGTGDDDDDADAMPMPDAVPDSPVIT
                     ISTNPDWPADSALPAEFDFPPYLNMLDASTVVVSWRTVNGSTGTVHFGKTPAYGTDLT
                     DTTSANLHHVTLASLEPATAYFYQVAIDGTSATRDGVFVTPGATSWRFMHSGEFHAPS
                     SSTNVAKYAAQIRAFRPHVLLESGDMVDVGSDLTHWRSYMRTSAPWISNVILLPAHSN
                     HVNGTGGNALLKDLFVLPNNERWYTTRYSQVEWVSIDSTYDDNADVATDEVPWIGTRA
                     AAAHDGVDDPTFLIAAWHYPACSSQYQSRSGERLWVYNNLVKAFTDNGGVDMILAAHD
                     KYYERSTITGGIVHVITNIGNISPAIAGSNNPACTPEKTIRTTRSTMFVNVDDKLLSA
                     KMFNETGTEIDAVSIMK"
     gene            complement(2403..4364)
                     /locus_tag="VMZ53_05650"
     CDS             complement(2403..4364)
                     /locus_tag="VMZ53_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine protein kinase/MFS
                     transporter"
                     /protein_id="HUS27968.1"
                     /translation="MAGDPERSDDEEQPVEQAEEQPDVVRNAPSSPRVTRLYGAVDLE
                     EAYPATLLDGGSVANDPAGTRAMTAPSPVVGSYLERYRIGDVLGQGGMGEVLSARDEQ
                     IGRPVAIKRLRVENPSPEVLARFLREARIQGRLEHPAVVPVHELITEEGEQPFFVMKQ
                     LAGTTLADVIPKLALRDKRAMEDFTLQRLLRAFADVSLAIEFAHTRHVVHRDLKPANV
                     VLGDFGEVYVLDWGIARITEDTSDDHRDSFSDIGTVAGTETVAGAILGTPGYIAPEQI
                     RGDFFLDGRADVYSLGCILFEILALQPLHPRGQGGLASALAGIDARPSIRAPDRETPP
                     ELDAICVKATALDPNQRYQTARELGAAVQHFLDGNRDVALRRDLAKSELATARAALKL
                     GNGPDQRRDALRAAARALALDPTNREPADLVGHLMLEPPVEMPEEVERELAGIDLEAL
                     KTSARFGTLAAMAYLAFFPILYLIGFHELWYMIAGPAVCLAIILIEILVAPRSPYLSG
                     YLAIVGNLMMFALFGWMVSPIVIGPGPAIVMVTLMATHRRLVRPWQLALLTCIATLTP
                     WLLEIAGVLESRVSVSGNTLQLHTAAGVLDPNATLAGLMIYIIALIHMAALLSRLQDD
                     DRRKTRRAMQLQSWTLRQLVPRATSKPPP"
     gene            4574..6241
                     /locus_tag="VMZ53_05655"
     CDS             4574..6241
                     /locus_tag="VMZ53_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HUS27969.1"
                     /translation="MGTYVHTQHDDFDDVVDRGAIPIIHDQHLAVTEARLSLDAGLTA
                     RFGASVMIPLRLISTSIVYRDGMGSEVQLANPNIHHRNETLTGLGDPMLLGAFASKYV
                     TARAGLTLPLGKTEEDPFAAGDMGLSHEHIQMGTGTVNPVVSLEGSYGWAPWKFGAYV
                     FTQQILYENSKGYQAGDRYAGGIFARRGFGAWGARVGVDVQGETSERWHGVRHTDEGN
                     QGRIDAMLSFGASWAATDRLALDAMVKVPFITHVVGGQLDMPALVEVGVAYRFGKTPV
                     RVHHHDDDEHDHDHGDEHDHGDEHDHGDEHAHGDGHDHGDDHDHGDDHGERPKLDTTG
                     ADVVDLEPPGAAVELVPVRGKLTIFEFGASWCEPCKTLEPALVELAKKHPDTVAVRRI
                     DVVDWDSAVAMKYLAPKAYDLPHVKIYDATGKLVFERSSAPGKLGKLIDDIRAIAEPQ
                     PPPQQSQTTPPSTKPAAVKPYRVAIEVTEKGFTPAEVKVPHDRPVVLVFTRKTEKTCA
                     NDVVIPGGIVKDLPLDKPVEIQVTFTKPGPVKYACGMNMIKGTIVVQ"
     gene            6251..7252
                     /locus_tag="VMZ53_05660"
     CDS             6251..7252
                     /locus_tag="VMZ53_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27970.1"
                     /translation="MGTVRVVVVIAVVALSTTADAGPTQCRVFNNTDHELVYYGAVRP
                     GEWFVISAPKEQVVDGSTANLEIPCSKKTRAIKLTGGKGSVSIEALPSWFDVVTTDGA
                     VRVTAPKTATVTIDGATVKVAKGVAVQKLDLRTRALQLDLADVFAGASGAWTYPINVA
                     ATLGKEQVSLSLLIDTASAGYALVDELRTVAKQPVTWAAEPDHLGPALIMGCPGVPHP
                     ITAKTRASEITKGYAGVRGVTNLTSVSMVAICEAKDVKLDECPKSKDPFAKNEKPATR
                     YRRDEIIHLYEAKSGKSVASTTLQGGAPPACRDTSPAMLRGEDVTKSAILAWMNGVK"
     gene            7302..7550
                     /locus_tag="VMZ53_05665"
     CDS             7302..7550
                     /locus_tag="VMZ53_05665"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HUS27971.1"
                     /translation="MNLTLAVDEALVERAREAARQQGTSLNALIRDFIEQLAGQTSGD
                     QLLADFEALWAEPGSSDGKYKFDREELYAGRLARYKRK"
     gene            complement(8017..10701)
                     /locus_tag="VMZ53_05670"
     CDS             complement(8017..10701)
                     /locus_tag="VMZ53_05670"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS27972.1"
                     /translation="MTRRASPWIVLPAALALAYAIAVVAVSWSAPEKGFKAFTGQRVV
                     DVDAAGVADVAGLREGDVIVSVDGTPIKSTLDYAFRVLRRNPGETVQVGVRRGSEQQE
                     IAIELEQSSPPWSAIVATLLSLAMIILGISARISRPDDDVDARRFYRTSIVYALVYVG
                     ALSWPRLIVHPVLAVVFLAALFVSPANSLGLSIDFPHHAKLNPSARLWTRLAWAFAIL
                     VGALCTVGLGLAIADFPDGGDRGLQLMVASVALQCGSVPVLAAVGLASQLRSHRIAHG
                     ELRAQLRWIIAGHILGIIPVLVAIPFAVTDIDRFLITRYQPFVVSIAVLWSLGYGFAV
                     LRIRLADVNVLLRSGIAYAATTTAAVGVYVGIVLAAGWITGSLVGDTGPLPHLIAGIC
                     AAVVFGPIRSATTRWLDRRFFRDRTHYLEALRRAGESLALLREPADLARESCEQIALA
                     LRAEYAALYTKAGVLYATPGAPAIDAAPLAAAPRDGIAVPVTDARTGSEPAWLVLGPR
                     KSGDLYSTDDRDLLGAVASQLAIALANAGAFGKIKALSRTLESQNEQIRDLRDKLEDE
                     NRFLRQRVEAATDGARLVGTSKAMRELQNTLERAAGSDATVLLLGESGTGKGLAARTL
                     HARSPRADKPFMQVDCGAIAASVFESELFGHERGAFTGATRTRRGPIELADGGTLFLD
                     EIGELPLDLQPKLLRMLEDRTVLRVGATAPVSVDVRIIAATNRDLAAMVKQGLFREDL
                     FFRLNVVSITVPSLRARRADLRELCEQLLPRVARRSGRDVRPLADDALTRMMAYAWPG
                     NVRELENVLGRALVLGDGAQITADDLDLPDRPVEDEAPDEIAAEASKPHDAVMDDIER
                     RRLSAALAAAEGNQSHAAKSLRMPRTTFINKLRRHGLL"
     gene            10742..>12220
                     /locus_tag="VMZ53_05675"
     CDS             10742..>12220
                     /locus_tag="VMZ53_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HUS27973.1"
                     /translation="MNRRTFLISAGATAVLLAFRSKLSFAKGDAAMPVKKLKPPTEPL
                     LAPWKGPYGGVPPFDKIKVKDFKPALMKGMDLSRADIKAIAENTEAATFENTIAAFED
                     AGRHYGRAGSMFGTFTSTMNDKAMQKVQKEMAPIMAAFSDEIIQNEALFARIKTVYEA
                     RNKSNLTPEQIRLVETHYTNFARQGAALNKDQKAKLAEINKKLAELFTAFQQNQLHDE
                     ESGTVVLEKEDELAGLPESLRAAMKNEAEGKKLKGKWVISNTRSSAEPFLTYSSRRDL
                     REKVFKMWNSRGDAAGEHDNKPVMQQILALRGERAKLLGFKSHAHWIIDDNMAKTPDN
                     AMSLMMRVWKAATARVKEEVADMQKVADAEKAGHKIEAWDYRFYAEKVRKAKYDLDQD
                     QIKEYLQLDKMREAMFWAAGQLYGLEFKQIKKGVPVYQKDMTVYEVVRDGKHVGLWYF
                     DPYARAGKNSGAWMSEYRTQEGFKDTVTPIVSNNCNYVKTKAG"
BASE COUNT         2272 a         3748 c         4199 g         2001 t
ORIGIN      
        1 tcgacggagc ggtgattgct tcgggcgcga gaaaggcggg cgtgccgagg atgacttgcg
       61 tcgtatcgcc gtcggcggcg atctctttcg cgaggccgaa gtcgacgacc ttcgcgacgt
      121 cggggaccgc gccgcgctcg cacaggatga tgttcgcggg cttgatatcg cgatgaatga
      181 cgcctcgatc gtgtgcttcc tgcagcgcgc cgcagacttg ctcgaggatg cgaatcacgc
      241 ggccgggacg ctgcggtcca gacgtgcgca cgagcttctc gaggtcgaca ccgtcgaggt
      301 actccatcgc gtaatagaag atgccgtcgg ggctgtggcc gtagtcgaac accgcgacgg
      361 tgtttggatg cgtgagctgc gacgtgtgct gcacttcgcg ctcgaaccgg cggagcgcgt
      421 cggcgccgat ccggcctttc tgcagaagct tgattgccgt tggccggcgc aacagcacgt
      481 gatgcgcgcg gtacacgacg cccattccgc cttcgccgat ctttgcgtcg agcgtgtagc
      541 gcccgagctt gacggctttg tcgaagttgc ggcggaggcc atggaggatg ctcgagccga
      601 gtgcggcgag cacggtcgcg acgctgctca gcacgaccgc gccgatgacg aacggtaggg
      661 gcggcagatc ttgttctacg gtgagcgcga ggacgatcga cgagtacacg aggcagccga
      721 gcagcagcga cgacaggatc gccgtgcgcc gcgcgctcga cggcaagatg atcgcgcgcg
      781 tgaacacgat gaatatgccg agcaggagcg ccgtgtaccc cgcgtcgtgc agctgccatg
      841 aatagatcgc gctacacgcg agccatacgc cgaagcacgc cgcgtaggcc gcgtcgacgg
      901 agtgcagcac tcgcatcgat cgaggagagc gcagaaacac gcgccctgcg gcaagcacgg
      961 ccacaaccgc gaatgcagca cggttgaggg aacccgcggc tggcggttcg atctctgggt
     1021 atctcgaata gatgagcgca acgagcagca tgaagccgcc gaagccccac gcgagcacga
     1081 ccgacagaac gcacagccgc gcctgcagat agctgcgcgc ctcttcgtcg tggtgccccg
     1141 ccgccgttcc cattctcgtt acttcataat ggatacagcg tcgatctcgg ttccggtctc
     1201 gttgaacatc ttcgccgaca gcagtttgtc gtcgacgttg acgaacatcg tcgagcgcgt
     1261 cgtgcggatc gtcttctccg gcgtgcacgc gggattgttg ctgcctgcga tggccggcga
     1321 gatgttgccg atgttcgtga tgacgtggac gatgccgccg gtgatcgtcg agcgctcgta
     1381 atacttgtcg tgcgcggcga ggatcatgtc cacgccaccg ttgtcggtga atgctttgac
     1441 gagattgttg tagacccaca gacgctcgcc ggagcgcgat tggtattgcg acgagcaagc
     1501 ggggtagtgc caggcggcga tgaggaacgt tggatcgtcg acgccgtcgt gcgcggcagc
     1561 ggctcgagtg ccgatccagg ggacctcgtc ggtcgcgacg tcggcattgt cgtcgtacgt
     1621 cgagtcgatc gagacccatt cgacttggct gtagcgcgtc gtgtaccagc gctcgttgtt
     1681 cggcaggacg aagaggtctt tgagcagcgc gttgccgccc gtgccgttga cgtggttcga
     1741 gtgcgctggg agaaggatga cgttggagat ccacggggcg gacgtccgca tgtagctgcg
     1801 ccagtgcgtg aggtcgctgc cgacgtcgac catgtcgcca ctctcgagga gcacgtgcgg
     1861 gcggaaggcg cggatctgcg ctgcgtactt ggccacgttc gtcgacgagg agggcgcgtg
     1921 gaattcgccc gagtgcatga agcgccagga cgttgcgccc ggcgtgacga agacgccatc
     1981 gcgggtggcg gacgtgccgt cgatcgcgac ttggtagaag taggcggtcg cgggctcgag
     2041 gcttgcgagc gtgacgtggt ggaggtttgc cgacgtcgtg tcggtgagat cagtgccgta
     2101 cgccggcgtc ttgccgaagt ggacggtgcc cgtcgagccg ttgacggttc gccagctcac
     2161 gacgaccgtc gatgcgtcga gcatgttgag gtacggcggg aagtcgaatt cggccggaag
     2221 ggcgctatcc gctggccagt cgggattggt cgaaattgtg atgaccgggg agtcgggcac
     2281 agcgtcgggc attggcatcg cgtcggcgtc gtcgtcgtcg tcgccggttc cagaatgaaa
     2341 cgccgagcaa ccgagcgcac ctagggacac caggacgagc caccgcaccc ctccagcgta
     2401 catcaaggcg gcggcttgct cgtggctcgt ggaacgagct ggcggagcgt ccatgattgc
     2461 agctgcatgg cgcgtcgcgt cttgcggcgg tcgtcgtctt ggaggcgcga gagcagggcg
     2521 gccatgtgga tcagcgcgat gatgtagatc atcaggcccg cgagcgtcgc gtttggatcg
     2581 aggacgcctg cggccgtgtg cagctgcaac gtgttgccgc tcaccgatac gcggctctcg
     2641 agcacgcccg cgatctcgag gagccatggc gtgagcgttg cgatgcacgt gaggagcgcg
     2701 agctgccatg gccgcacgag gcggcggtgc gtggccatga gcgtaaccat cacgatcgcg
     2761 gggccggggc cgatcacgat cgggctgacc atccagccga acaacgcgaa catcatgagg
     2821 ttgccgacga tcgcgaggta gccggagaga tagggactcc gtggcgcgac gaggatctcg
     2881 atgaggatga tcgcgaggca aactgccgga ccggcgatca tgtaccagag ctcgtgaaag
     2941 ccgatcagat agaggatcgg gaagaacgcg aggtaggcca tcgctgcgag cgtgccgaac
     3001 cgcgcgctcg tcttgagcgc ttcgaggtcg atgcctgcga gctcgcgctc gacttcttcg
     3061 ggcatctcga cgggaggctc gagcatgaga tgaccgacga ggtccgcggg ctcgcgattc
     3121 gtcggatcga gtgcgagtgc gcgtgccgcg gcacgcaggg catcgcgtcg ctggtcgggg
     3181 ccgttgccga gcttgagcgc ggcgcgtgct gtggcgagct ccgacttggc gaggtctcgg
     3241 cgaagcgcga cgtcgcgatt gccgtcgagg aagtgctgga ccgcagcgcc aagctcgcgc
     3301 gcggtttgat agcgctggtt cggatcgagc gcggttgcct tgacgcagat cgcgtcgagc
     3361 tcgggcggcg tttcgcgatc cggtgcgcga atgctcggtc tcgcgtcgat ccctgcgagc
     3421 gccgaggcga ggccaccttg cccgcgcgga tgcagcggtt gcagcgcgag gatctcgaac
     3481 aggatgcagc cgagcgagta gacgtcggcg cgcccgtcga ggaagaagtc gccgcggatc
     3541 tgttcgggcg cgatgtagcc gggcgtcccg aggattgcgc cggcgaccgt ttcggtgcca
     3601 gcgaccgtac cgatgtccga gaagctgtcg cggtgatcgt cgctcgtgtc ctcggtgatg
     3661 cgcgcgatgc cccaatcgag cacgtagact tcaccgaaat cgccgagcac gacgtttgct
     3721 ggcttcagat cgcgatgcac gacatggcgc gtgtgcgcga actcgatcgc gagggagacg
     3781 tcggcgaatg cgcgcaagag tcgctgcagc gtgaagtctt ccatcgcgcg cttgtcgcgc
     3841 agggcgagct tcgggatcac gtcggcgagc gtcgtgcccg cgagctgctt catgacgaag
     3901 aacggctgct cgccttcttc ggtgatgagc tcgtgcacgg gcacgacagc cggatgttcg
     3961 agacggcctt ggatgcgcgc ttcgcgcaga aagcgtgcga gaacttccgg gctcggattc
     4021 tcgacgcgca gtcgtttgat cgcgacgggc cggccgatct gttcgtcgcg cgccgacaga
     4081 acctcgccca tgccgccttg gccgagcaca tcaccgatgc gataccgctc gagatacgag
     4141 ccgacgaccg gactcggcgc tgtcattgct cgcgtgcccg ccggatcgtt cgcgacactg
     4201 ccgccgtcga ggagagtcgc gggatacgcc tcttcgagat cgacggcgcc gtatagacgc
     4261 gtcacgcgtg gcgatgaagg cgcgttgcgc acgacgtccg gctgctcttc cgcctgctct
     4321 accggctgct cttcgtcgtc ggaccgctcc gggtcgccgg ccatgacata tcgtaaccgg
     4381 acacgcgtgc cagttgcgct atggtgcccg tatgcatttt cctagggctg cactgctgct
     4441 tgcgttggtc ctcgcaccac gcatcacata cggcggcggt gcttgagcgg gttgaagtaa
     4501 cccaaacctc ccggtcggga aggtagacag cgacgcgctc gccccagggc aggtagtcct
     4561 gcgcctaggg tggatgggta cctacgtcca cacgcagcat gacgacttcg atgacgtcgt
     4621 cgaccgcggc gcgatcccga tcatccacga ccagcatctt gcggtcacag aagcgcggct
     4681 ctcgctcgac gccggtctga ccgcgcggtt cggcgcgagc gtgatgattc cgctccgcct
     4741 gatctcgacg tcgatcgtct atcgcgacgg catgggctcc gaggtccagc tcgcgaatcc
     4801 caacattcac catcgcaacg agacgctcac cggcctcggc gatccgatgc tgctcggcgc
     4861 gttcgcgtcg aagtacgtga cagcgcgcgc gggcctcacg cttccgctcg gcaagaccga
     4921 agaggatccg ttcgccgcgg gcgacatggg cctctcgcac gagcacatcc aaatgggcac
     4981 cggaaccgta aatccggtcg tgtcgctgga gggctcctac ggctgggcgc catggaagtt
     5041 tggtgcgtac gtattcacgc agcagatcct ctacgagaac agcaagggct accaagcagg
     5101 cgatcgctac gcgggtggca tcttcgcgcg acgaggattt ggcgcgtggg gagcgcgcgt
     5161 cggcgtcgac gtgcaaggcg agacgagcga acggtggcac ggcgtgcgcc acaccgatga
     5221 aggaaaccaa ggccgcatcg acgcgatgct ctcgtttggg gcgtcgtggg cggcaaccga
     5281 tcggctcgcg ctcgacgcga tggtgaaggt cccgttcatc acgcacgtcg tcggtgggca
     5341 gctcgacatg cccgcgctcg tcgaggttgg cgttgcatat cgattcggca agacgcccgt
     5401 gcgcgtccat caccacgacg acgacgagca tgaccacgat catggcgacg agcacgatca
     5461 tggcgacgag cacgatcatg gcgacgagca cgctcatggc gacggccacg atcacggcga
     5521 cgaccacgat cacggcgacg accatggcga acggccgaag ctcgacacga ccggcgcgga
     5581 tgtcgtcgat ctcgagccgc ccggcgccgc cgtcgagctc gtgcccgtgc gcggcaagct
     5641 gacgatcttc gagttcggcg cgtcgtggtg cgagccgtgc aagacgctag agcccgcgct
     5701 cgtcgagctc gcgaagaagc accccgacac cgtcgcggtg cgccggatcg atgtcgtcga
     5761 ttgggacagc gcggtcgcca tgaagtacct cgcgccgaag gcgtacgacc tgccgcacgt
     5821 gaagatctac gacgctaccg gcaagctcgt gttcgagcgt tcgagcgcac cggggaagct
     5881 cggcaagctc atcgacgata ttcgagcgat tgccgagccg cagccgccgc cgcagcaatc
     5941 gcagaccacg ccgccatcga cgaagcctgc cgccgtcaaa ccgtatcgcg tcgcgatcga
     6001 ggtgacggag aagggcttca cgcccgctga agtgaaagtc ccgcacgacc gtccggtggt
     6061 gctggtgttc acgcgcaaga ccgagaagac gtgcgcgaac gacgtcgtca ttccgggcgg
     6121 catcgtgaag gacctgccgc tcgacaagcc cgtcgaaatt caggtgacgt tcacgaagcc
     6181 gggtcccgtg aagtacgcgt gcggcatgaa catgatcaag ggcaccatcg tcgtgcagta
     6241 agctctccag atggggacgg tacgagtcgt cgttgtaatc gccgtcgtgg cgctttccac
     6301 caccgcggat gcgggcccca cgcagtgccg cgtgttcaac aacacggatc acgagctcgt
     6361 ctattacggt gcggtgcgtc ctggagagtg gttcgtcatc agcgcaccga aggagcaggt
     6421 tgtcgacggc tccacagcga acctcgagat tccgtgctcg aagaagacgc gcgcgatcaa
     6481 gctcaccggt ggaaagggct cggtcagcat cgaagcgctt ccgtcgtggt tcgatgtcgt
     6541 cacgacggac ggcgccgttc gcgtcacggc accgaagacc gcgacggtga ccatcgatgg
     6601 cgccacggtg aaggtggcga agggcgtcgc cgtgcagaag ctcgatctca gaacgcgcgc
     6661 gctccagctg gatcttgccg atgtcttcgc cggcgcaagc ggagcgtgga cctatccgat
     6721 caacgtagcc gcgacgctcg gcaaggagca ggtctcgctg tcgctgctca tcgacacggc
     6781 atctgctggt tacgcgctcg tcgacgagct tcgaaccgtc gcgaagcagc ctgtcacctg
     6841 ggcggcagag cccgaccatc taggacccgc gctgatcatg ggctgtcccg gcgttccgca
     6901 cccgatcacg gcgaagacgc gcgcatcgga gatcacgaaa ggctatgccg gcgttcgcgg
     6961 cgtgacgaac ctgacgtcgg tctcgatggt cgcgatctgc gaggccaagg acgtaaagct
     7021 cgacgagtgt ccgaagtcga aagatccgtt cgcgaagaac gagaagccgg cgactcgcta
     7081 tcgccgcgac gagatcattc atctctacga agcgaaatcg ggcaagagcg tcgcttcgac
     7141 gacgctgcag ggcggagcgc cccctgcatg ccgcgatacg tcgccggcga tgctgcgagg
     7201 agaagacgtt acgaagtcgg cgatcctcgc ctggatgaac ggcgtgaaat gacgtctaga
     7261 gaccggttgc cacggaataa ggtcacacgt attatgtgtg aatgaacttg acgctcgcag
     7321 tggacgaagc gctggtcgag cgcgcgcggg aagcggcgcg gcaacagggg acgagcctga
     7381 atgccttgat tcgcgacttc atcgaacagc ttgccgggca gacgagcggt gaccagctgc
     7441 tcgccgactt cgaagccctt tgggccgagc ccggaagctc ggacggtaag tacaagttcg
     7501 accgagaaga gctctacgcc ggacgcctcg ctaggtataa gcgcaagtga cggatcgagt
     7561 gttcatcgac acgaacttgt tcgtgtatgc cgatgacgcg aaggcggcga cgaagcgaga
     7621 ccgagcgcgc gctgtcttgt cgccgctgat tcgctcgcgt cgcgcggtcg tttcgacgca
     7681 gatcctgcag gagtacttca gcgctgcaac gaagaagcta gggcttcctg cggagcgcgc
     7741 ccgttggcga gtcgagacga tggcgcgctt ggaggtcgta gtcattcggc cggagctcgt
     7801 actcgcggcg atcgacctgc atcgtctcca ctcgttttca ttgtgggacg cgctcgtcat
     7861 cacatgcgcc gcggcaggca actgctcgcg tgttctcacg gaggatctcc agcacgggca
     7921 gaccatctcg ggcgtgcaga tcgaaaatcc attcgccact acgagtcggg cctcagagcc
     7981 gcgagcgcga taccgaagtc gggcgcgagc gcgagtctag agcaggccgt gccggcgaag
     8041 cttgttgatg aacgtcgtgc gcggcatgcg gagcgatttc gcggcgtgtg actgattgcc
     8101 ttcggctgca gcgagcgcgg cggagagccg gcgacgttcg atgtcatcca tgacggcatc
     8161 gtgcggcttc gacgcttcgg ccgcgatctc gtcgggggct tcgtcttcga cggggcgatc
     8221 gggcagatcg agatcgtctg cggtgatttg tgcaccgtcg ccgaggacga gcgcgcgacc
     8281 gaggacgttt tcgagctcgc gcacgttgcc gggccacgcg tacgccatca tgcgagttag
     8341 cgcgtcgtcg gcgagcgggc ggacatcgcg accggagcgg cgtgcgacgc gaggcagtag
     8401 ttgctcgcag agctcgcgca ggtcggcgcg acgcgcgcgc agcgacggca cggtgatcga
     8461 gacaacgttg aggcggaaga ataggtcttc gcggaacagg ccctgcttca ccatcgccgc
     8521 gaggtcgcgg ttcgttgcag cgatgatgcg aacgtcgacc gagaccggag ctgtcgcgcc
     8581 gacgcggaga accgtgcggt cttcgagcat gcgcaggagc ttcggctgca gatcgagcgg
     8641 cagctcgccg atctcgtcga gaaacagcgt gccgccatcg gcgagctcga tcgggccgcg
     8701 acgcgtgcga gtcgcgccgg tgaatgcgcc acgctcgtgt ccgaacagct cgctctcgaa
     8761 gacggaggct gcgatggcgc cgcagtcgac ttgcatgaac ggcttgtcgg cgcgtgggga
     8821 cctggcgtgg agcgtgcgcg cggcgagacc cttgcccgtc cccgactcgc cgagcagcaa
     8881 cacggtcgcg tcgctgcccg cggcacgctc gagcgtgttc tgtagctcgc gcatcgcttt
     8941 cgacgtgccg acgagcctcg cgccatcggt ggcggcctcg acgcgctgac gcagaaagcg
     9001 attctcgtct tcgagcttgt cccgcaggtc gcggatctgc tcgttctgac tctcgagcgt
     9061 gcgcgacagc gctttgatct tgccgaacgc gccggcgttc gcgagggcga tggccagctg
     9121 gctcgcaacg gcgccgagga gatcgcgatc atcggtcgag tagaggtcgc cgctcttccg
     9181 cggaccgagg acgagccatg ccggctcgct tcccgtgcgt gcgtcggtga ccggaacggc
     9241 gatgccatct cgaggagctg cggcgagcgg agcggcgtcg atcgcgggcg cgccgggtgt
     9301 cgcgtagagc acgccggctt tcgtgtagag cgccgcatac tcggcgcgta gcgcgagcgc
     9361 gatctgttcg caggattcac gcgcaagatc cgccggctcg cgaagcaatg cgagtgattc
     9421 gccagcgcgg cgcagcgctt cgaggtagtg cgtgcgatcg cggaagaacc ggcgatcgag
     9481 ccagcgcgtc gtcgccgagc gaatggggcc gaagacgacc gccgcgcaga tgccggcgat
     9541 gaggtgcggc agtgggcccg tgtcgccgac gagcgagccg gtgatccagc cggccgcgag
     9601 cacgatgccg acgtagacgc caaccgccgc ggtggtcgtc gccgcgtatg cgatgccact
     9661 tcgcagcagc acgttgacgt ccgcgagccg gatgcgcagg acggcgaagc cgtagccgag
     9721 cgaccacagt acggcgatcg aaacgacgaa cggctgatag cgcgtgatca gaaaccgatc
     9781 gatgtcggtg accgcgaacg ggatcgcgac gagcaccgga atgatgccga ggatgtggcc
     9841 ggcgatgatc cagcgcagct gcgcgcgcag ctcgccgtgt gcgattcgat gagagcggag
     9901 ctgcgatgcg agaccgaccg ctgcgagcac tggcacactt ccgcattgca gtgcgaccga
     9961 tgcgaccatg agctgcagtc cgcgatcgcc gccgtcgggg aaatcggcga tcgcgagacc
    10021 caagccgacc gtacacagcg cgccgacgag aatcgcgaac gcccacgcca ggcgcgtcca
    10081 cagtcgcgcg ctgggattga gcttcgcgtg atgcggaaaa tcgatcgaca gcccgagcga
    10141 gtttgcgggc gacacgaaca gcgccgcgag aaacacgacg gcgagcacgg gatggacgat
    10201 gagccgcggc cacgacagcg cgccgacgta gacgagcgca tacacgatgc tcgttcgata
    10261 gaagcgccgc gcgtcgacat cgtcatctgg ccgcgagatg cgcgcggaga tgccgaggat
    10321 aatcatcgcg agcgagagca gcgtcgcgac gatcgcggac cagggcggcg acgattgctc
    10381 gagctcgatc gcgatctctt gctgctcgct tcctcggcga accccgacct ggacggtctc
    10441 gcctgggttt ctgcgcagca cgcgaaatgc gtagtcgagc gtgctcttga tcggcgtgcc
    10501 gtcgaccgaa acgatcacgt caccctcgcg cagccccgcg acatctgcga cacctgcggc
    10561 atcgacgtcg acgacgcgtt gacccgtgaa agccttgaaa cctttctcgg gtgcgctcca
    10621 cgagaccgcc acgaccgcga tcgcgtacgc cagggccaat gcggctggca gcacgatcca
    10681 gggcgaagcc cgtctggtca cggtggagga ataaccgatc agcggctata tatgcgggcg
    10741 aatgaatcga cgcacgttcc tcatctccgc gggcgccacc gcggtcctcc tcgctttccg
    10801 cagcaagctg tcattcgcaa agggagacgc ggcgatgccc gtcaagaagc tcaagcctcc
    10861 gaccgagcca ctgcttgccc cgtggaaggg gccctacggc ggagtgccgc cgttcgacaa
    10921 gatcaaggtg aaggacttca agcccgcgct gatgaagggc atggatctct cgcgtgcgga
    10981 catcaaggcg atcgccgaga acaccgaagc cgcgacgttc gagaacacga tcgccgcatt
    11041 cgaagacgca ggtcgccact acggccgcgc cggctcgatg ttcggcacgt tcacctcgac
    11101 gatgaacgac aaggcgatgc agaaggtcca aaaggagatg gcgccgatca tggcggcgtt
    11161 ctccgacgag atcattcaga acgaggcgct gttcgctcgc atcaagaccg tctacgaggc
    11221 gcgcaacaag tcgaacctga cgccggagca gattcgcctc gtcgagacgc actacacgaa
    11281 cttcgcgcgg cagggcgcag cgctcaacaa agaccagaag gcgaagctcg ccgagatcaa
    11341 caagaagctc gccgagctgt tcaccgcgtt ccagcagaac cagctgcacg acgaagagag
    11401 cggcacggtt gtcctcgaga aagaagacga gctcgccggt ctgcccgagt cgctgcgcgc
    11461 agcgatgaag aacgaagccg agggcaagaa gctcaagggc aagtgggtga tctcgaacac
    11521 gcgctcgtcg gccgagccgt tcctgaccta ctcgtcgcgt cgcgacctgc gcgagaaggt
    11581 cttcaagatg tggaacagcc gcggtgatgc cgcgggcgag cacgacaaca agccggtcat
    11641 gcagcagatc ctcgcgctgc gcggtgagcg cgcgaagctg ctcggcttca agtcgcacgc
    11701 tcactggatc atcgacgaca acatggcgaa gacgcccgac aacgcaatgt cgctgatgat
    11761 gcgcgtgtgg aaggccgcga cggcgcgcgt gaaggaagaa gtcgccgaca tgcagaaggt
    11821 cgccgacgcc gagaaggccg gccacaagat cgaagcgtgg gactaccgct tctacgcaga
    11881 gaaggtccgc aaggcgaagt acgacctcga tcaggatcag atcaaggagt acctgcagct
    11941 cgacaagatg cgcgaggcga tgttctgggc cgcgggccag ctctacggcc tcgagttcaa
    12001 gcagatcaag aagggcgtcc ccgtctacca gaaggacatg accgtctacg aggtcgtgcg
    12061 cgacggcaag cacgtcgggc tttggtactt cgatccatac gcgcgcgcgg gcaagaactc
    12121 gggcgcgtgg atgagcgagt accgcacgca agaaggcttc aaagacacgg tgacgccgat
    12181 cgtgtcgaac aactgcaatt acgtgaagac gaaagcgggc
//
LOCUS       DATLBZ010000109        19437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_848584, whole genome shotgun sequence.
ACCESSION   DATLBZ010000109 DATLBZ010000000
VERSION     DATLBZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19437
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848584"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VMZ53_05680"
     CDS             complement(<1..627)
                     /locus_tag="VMZ53_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27974.1"
                     /translation="MTSLATASGTSAPPLTSQATQAFAVSVLDRHESELVQQAMEAVA
                     SRSPNDAVILQGLISELRATSELLDRQRPLRRPTQLGGEQRDEQTLIDHLCDLDGLSG
                     DLVLPLKATLSRTHLLTKINFLRGFVKATSALRDVPTAVKMTHELREEVAQSIYTLLA
                     EELFLALLRKPDVSRRTKQRAADQLITVWDDAALEIDDFAPLLESAWHA"
     gene            complement(710..1342)
                     /locus_tag="VMZ53_05685"
     CDS             complement(710..1342)
                     /locus_tag="VMZ53_05685"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HUS27975.1"
                     /translation="MHAFAEFLQDIVARMGYPGVFLLVMLESTLVPVPSELVFPFAGY
                     MASKGVFNIYVLLVINSVAALAGSSIGYWIGAAGGKPLLLKYGKFLLIRKHDIEKTEK
                     WFANHGKATIFIARLVPVIRHIISLPAGVARMPFRSFLLQTFLGSTLWGSFLILLGYY
                     LGKNWESVANKLKRFDLVIGVVVVVAVMAIAVRWYLRRRRERGQAENNAD"
     gene            complement(1361..1822)
                     /locus_tag="VMZ53_05690"
     CDS             complement(1361..1822)
                     /locus_tag="VMZ53_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27976.1"
                     /translation="MHVQESADALLLTGAIDEHAELMALLGRARGGKLVLDLGGITFI
                     NSLGVRDWIRMQASATQQGIAIEMRRVAEVLIHQLNMIIATRGTAKVTSFFAPFACDA
                     CGREESLLIDAVANAAGLAQLQPPTMTCPECGAAMAFNDFPERYFSFLTST"
     gene            complement(1846..2775)
                     /locus_tag="VMZ53_05695"
     CDS             complement(1846..2775)
                     /locus_tag="VMZ53_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27977.1"
                     /translation="MHTILIVHPDRKTQRTVQRILGITGHRIEIADTLEQATRLMQHS
                     PPLVVVVAGNIAMNADGEAFLAQARAAGAEACMTLLGESAPDHVPHILGLGAVTNLLV
                     HPMPVLGEELTVTVQKLVRGDLFGAEKYLLWGTELHSQKITKASERSRIVEELANDVR
                     ARGQSARVASMARLVADELISNAVHNAPVDVQGHHPRKDTPRNVEIELDERHAVQLRW
                     GCDARYLALEVNDQFGSLDRDTILRSLAKNAVTESGAGAGMGISLSYRSCDHLVFNLS
                     PGKRTEIIALIDVRYPPSERAPVSSYNVFVERP"
     gene            complement(2778..4052)
                     /locus_tag="VMZ53_05700"
     CDS             complement(2778..4052)
                     /locus_tag="VMZ53_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS27978.1"
                     /translation="MATALTADHRVWRARVFVATWLSYVGFYFCRKPFSVAKKAMETQ
                     NHWHKAVTVGNIMAAYLIAYAIGQFLSSRIGTKLGPRNNLLIGMALSVLVTLIMGIPL
                     SPWILAGLIAVNGVAQATGWSSNVGTMANWFHKHERGHVMGLWGTNFVVGSISATFAL
                     GYLLGDVSPAEQDWQATFFVGAVVLAAIWVLFYYFQRNQPEDVGLPAIDDPVTAVDES
                     REQPAENLTRDQWTNILIVGGFYFFSKLIRYAVWSWSAYFLSSNYKLSGKDAAIYSVL
                     FDIAGIPGIWVTGFLSDRFFKSKRGGIALIMMLCMSVVTLMLVMFGNTSVVVFCLLLG
                     LVGFFLYGPDALLSGAGAMDIGSRKTAMFATATIAMLGALGPIVQEVIIPRFYKEDDL
                     SVVFVILFISALMGSVFCALLVWRNRRGGKGI"
     gene            complement(4053..5207)
                     /locus_tag="VMZ53_05705"
     CDS             complement(4053..5207)
                     /locus_tag="VMZ53_05705"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27979.1"
                     /translation="MRIGELLVEQRKLRQSELTRALADKPSDKRLVSYLIAKGLVDFD
                     DAARALGEQRRVPCALAKHLAGRDPELAKLIPAELGRSAWALPIGRASNGHIIVCVRD
                     PAPALLAQLQRALKTDVTMVITPASRLENLVRESYGDAPNDEFDIDFSSQVEDNPITP
                     AKPSPTPAPPPPLPDMAALDPESIRLSLTDLDDDRVEKDPTQSGQIPIVGLAAASTPP
                     RGHPRARTDPAATRKMSVGMMQVGLEHATSREAATDLVLAYVGTRWQTAVVFAIRDRT
                     AVGYRGHNVIAPESITMPLGAPSTLQHAIDSRFVSIQAPAGPTQDQLTRALEGAAHPA
                     AAPVMVKGQAAAVLVVGDPIDTSEDFQVAAADLAMLAEALGTAYQRILGR"
     gene            5255..5791
                     /locus_tag="VMZ53_05710"
     CDS             5255..5791
                     /locus_tag="VMZ53_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27980.1"
                     /translation="MLELAVLAMLVGVAGTAALAGLAGALPDERKVKREIATQRVRPI
                     RTLVDGANVAIRGEVAPAREGLTKAPLTGRACVYWVLQDGRRRKVGGHPFVLRDSTGE
                     TRVVPIDARLAVPPVHQQVDDGLVEYAVLPGATVTVVGVVTYEPDPDAVAAVTGYRKE
                     LPTRPVVSGTRRQRLLIG"
     gene            complement(5788..7794)
                     /locus_tag="VMZ53_05715"
     CDS             complement(5788..7794)
                     /locus_tag="VMZ53_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HUS27981.1"
                     /translation="MRKILIALLATAACSIPDKQPLGDDNGSGEEGTGELQTEITDAP
                     SDFSSSSVATFTFKANVPSATFQCTLDNGAPEPCTSPFSRSLPDGSHMFSVRATDTSG
                     NTDDSPAEHLWSIDTVAPSTMITEAPAVADNSTMVTFRFTSGEMNVSFECSIDNGAFA
                     PCRSGDQFGPISDGAHAFAVRAKDRAGNVDSSPAIHAWSVDTSTPDTTLIDGPVGASN
                     STSATFTFVSPDAGTGATFECSLDGGAFATCVSPHGYSNLSMGQHTFQVRVRDAVGNY
                     DPSPATRTWIIDATPPETMITAAPSGSISMTSAAISFTANENDVTFECSLDGTAMANC
                     TSPYNAMNLGQGAHTFSVRATDAAGNTDSSAATATWTVDTVAPNIMITGGPGNGDTVG
                     PRVVYTFSASEGTVTCSVGGAAARPCTSPFGFNSAAGNQSFTINATDAAGNMTTISRS
                     FTITCSAPDPAGSLGLLHLDDNGQTLANATGGASATLGNTDQAEAIDPMFTSGRFSGG
                     LAFDANQGDLVAWPLAAGSTSNFTIELWASPASLSGTRDILLSGDGRIAVRVTGAGGN
                     VTFSAAVIDNASATHTVTSAPVAAGAWHHVIASLSDATLRLWVDGVRTEIGDARPGTA
                     PALDSVRLGGAYGGSLDEVFLSATPIGDDESARGRFCPASGVVY"
     gene            complement(7794..8771)
                     /locus_tag="VMZ53_05720"
     CDS             complement(7794..8771)
                     /locus_tag="VMZ53_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27982.1"
                     /translation="MLPSAAHAQSAGAEALFRDGRTLIKQGKLDAGCDKLEASEKLES
                     SVGTLLNLGNCREKQGRVATAWAAFRKAESMAKLAGNDKKRQLEAKKRADKLEEALAT
                     ITIQVGPKSKLDGLVIKRDGETLDPAVYGTGIVVDPGQHTIVAEAPNAKPWKTDVAVG
                     KGGKRWVVVPSLEPVAEAKPVPPPVTPPPRVVTAPVSEPSTVVVTRPKTVVVEKTWST
                     TRGVAVGFAVLGAGALAGGVYFGKHAQTLEAKSDAICPETLCGDDEGLRLNREANTSA
                     RNANILLIGGGAAVAAATVMWFVGKPDTETVVAPSLGSDHVGATLSRRF"
     gene            complement(8806..10263)
                     /locus_tag="VMZ53_05725"
     CDS             complement(8806..10263)
                     /locus_tag="VMZ53_05725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS27983.1"
                     /translation="MNVESHIGQYKILRTLGAGGMGTVYLGEHILLGRRAAIKTLMPA
                     LSSHHEIVDRFFNEAKAISAISDPGVVQIFDFGYHVDGTAYIVMEMLEGESLSTRLER
                     LHQLTIGDALRIARQITASLASAHALGIVHRDLKPGNVFIIRDAEAQSGERTKILDFG
                     ICKLGDGASEDGSVTQTGQMLGTPVYMSPEQCRGAGKVDHRTDIYSVGCALYHMLTGQ
                     PPFNCESVGEFIAAHLKEDAATPSEIDPTIPPSVDALVMRCLAKDPANRFQSMKELHG
                     AIERALAEISDHGARLTAAAMNQTPLATGFHSQYDVNEGRQVAAEVTPSAASWFVDSM
                     SVPASSISDEWEAAPPPPRRRLGRTFMFAAALFIGIVGGLNATSYALDDKRPASEPPM
                     PLALTTEEPRVAAGGIVAHETAPPELEDLVASLPDEEEAPAPKQAPAPKEVTVQRPAK
                     VTRPPRPYVQRPPQPRRHVPASTTQQDDEDLYDAR"
     gene            complement(10362..11255)
                     /locus_tag="VMZ53_05730"
     CDS             complement(10362..11255)
                     /locus_tag="VMZ53_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS27984.1"
                     /translation="MRLHHINCISSCPLGGALMDRRSTGLRGKLACHCIVIETDRGLV
                     LLDTGFGLLDCARPTQRLSRFFLSLLDPDFREELTAIRQIEAMGYHAEDVRHIVLTHL
                     DFDHAGGLDDFPEATVHMLHDEASDAVRQRSWLDRQRYRPQQWGSYDRWQTYDSTAGE
                     RWYGFSCVRDIPGLPPEVLMVPLVGHTMGHAGIAVREDSGRWLLLAGDAYFDQDEMHP
                     SQPRCAPGLRAYQWMMEKDREQRLANQERLRQLVRDHAVEVQVVCSHDPRELERDTGH
                     TLDTPILGAVQSGISAAYPAR"
     gene            complement(11306..11746)
                     /locus_tag="VMZ53_05735"
     CDS             complement(11306..11746)
                     /locus_tag="VMZ53_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27985.1"
                     /translation="MRTVLLALLATAACRGDISRDIDNRAPAPAPKPVETTPADRDTA
                     IVPDDDHARVRDTDHEYVRVRSSFETSSRERLAKLDARINELEQSASESAHQTAAELR
                     KDREELAVRLESAREQAKPAWDEFQSNVEDSFQRLEKRLDDAVH"
     gene            11902..13476
                     /locus_tag="VMZ53_05740"
     CDS             11902..13476
                     /locus_tag="VMZ53_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27986.1"
                     /translation="MDPRALVRAAVAGGLLIISISASGCGSPQTADETDANPGPNFST
                     DGGGVGSDPGTPENPPAPKTFDVALVPDGASGTQRVNFAVPVAPGVIADASLIKITAG
                     GFELPAARRALAKYADGSVRSVQLQVDVDVTATHALTVELGAAGMTGPSLAAVSTTTT
                     GTGNNVHPKVWALLPTSVLIGSGLGGTLVARADQTIDAWDAICDYDKWDTAAFLVNAS
                     ASRDVWLYDRVTAMYRGYFVTGTISPLRSAYREAEIYRAGMVITNGVATAIAPPTAND
                     DLKYYYAQNMAMHYLLTGDDRFREAAEAVSARIASMWNPQYNGADRFWTERHAGFTLL
                     AHEWALMVTDDQAQTIAARSEAAVTAYLATQMTYPTGYTDQSARCFGHSATAHGEGYG
                     YNGCSPWMSAILADALDQYARRIGGQRAVEVRASLAMLGRSLARDGRDSTGKPFYWMG
                     EGTTQDEVDDYDEHWGESAYVIALAWNATGRTEASLKTAADQLVAGLESDGVVGQVRS
                     FNWQCRSAIATPALLK"
     gene            complement(13477..14652)
                     /locus_tag="VMZ53_05745"
     CDS             complement(13477..14652)
                     /locus_tag="VMZ53_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUS27987.1"
                     /translation="MGAAPADPESEGPDDDGSEVDADALADASPAPAVGTPFSIGGIP
                     GWVHFTNPAAHAGHDTNIIRETIRLIDATPKGETIHAAIHSLVLNNVVTALVAAKQRG
                     VTVLVAEDGSDEFDADDSPRQLAAALGANHVFCGDRRANGNFGCITTDSSGIMHTKLM
                     TFSKTAAPDGTLRTNVTWFGSANMTHATGENTFNNTITIYGDTELYTKLDKYFMQLFQ
                     QKHFANNDYYNADADRGFVVSPTTRIYLSPEQDSDLIKNRLNDIVPGASCEVRAAQAM
                     IFDSRPDLIDQLIRVKKGGCLVRVATGHIQSQSLARLKAANISVKHAKLHDKYVLVDA
                     KFADSTARRTIVFTGSHNWTKSANYANDELFSRLEDQAVYDAFLAHFRAAFESGTAP"
     gene            complement(14821..14997)
                     /locus_tag="VMZ53_05750"
     CDS             complement(14821..14997)
                     /locus_tag="VMZ53_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27988.1"
                     /translation="MLRKHASLIIAMSLVALVATACIVTTRRPPAQRGQPVYVEKHKE
                     PKPEKHKKPKKHKD"
     gene            15042..15677
                     /locus_tag="VMZ53_05755"
     CDS             15042..15677
                     /locus_tag="VMZ53_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27989.1"
                     /translation="MDWRALRRWLLAGVAVVAVLGLAAELAHAYAPTDLGEALLPKLS
                     LSYEQNLPTWFSSSLLLLCAIAAGAIASRPPPMRLHWWGMAVVAGWMSLDEAAEIHEH
                     LAGHFNTHGVLYFDWVIPATAIVIALALVYVPFVRRLESATRTRLIIAATIYVGGALM
                     MELPLGWITESRGIENLAYALVDWVEETMEMIGAALAFVALVAHRNSGVQR"
     gene            15674..16690
                     /locus_tag="VMZ53_05760"
     CDS             15674..16690
                     /locus_tag="VMZ53_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27990.1"
                     /translation="MKPRSPFIVSFTYDWIFFLAAPMLALGLGILVSDRPALSVSEYS
                     FGGERDNLVGFFTGTIVHAHLVAVLFRSHGNASIRRLYPIRFLVVPVVLWLAIVVSPW
                     IAVASTVVATFWDVWHSGAQTFGFARIYERNAGTPPELGRRLDFVLQQVLYAGPVLAG
                     ATLVDHLDSFSNFDHIGSTLLSSVPTSIEAHHRELAYAVIGVATVVIAVYVLVYWQLA
                     KRGAWSPPWLKIWLVASTGFVSIYTWGFNSWGQAFLIMNLFHAVQYLALVWAMEGTRI
                     AGMMRLPKRAAFCVYFGGVVVYGLGVQALDPDITTLWAITMVVSLMHFWYDAFVWSVR
                     RAQV"
     gene            complement(16607..17827)
                     /gene="ubiG"
                     /locus_tag="VMZ53_05765"
     CDS             complement(16607..17827)
                     /gene="ubiG"
                     /locus_tag="VMZ53_05765"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: protein motif:HMM:TIGR01983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HUS27991.1"
                     /translation="MLAACLVPIGLQALAMIVDEGVFHRRREMPRWERIGHPLDTLTI
                     VACLGWLLAGGGLTGYIVLAIASTLFVTKDEGVHAKLCGAGEQWLHAVLFALHPIVLA
                     AFGYLAHLGERGILTGQLAVTIAFMTYQIVYWNLPRRTRTINNDWYADLGERWYHAED
                     TPIALLRAESRQRNPWIADEIVRALGPASQRVLDLGCGGGFLANFLAARGHVVTGIDT
                     TAENLAVARAHGTATYDVADACALPYAAASFDVVCAMDLIEHVPEPDRLIAEVARVLR
                     PGGVFFFHTFNRTKIAHLIVIKGVEWFVKNTPNDLHVIDLFRSPAEVSTMCQAHGLEI
                     ATLHGSRPRFRWPLFRMLLTGRVGNDFAFTFTKSTAIGYTGFARRLDLSATDAPHERV
                     VPEVHERHDHGDRP"
     gene            complement(17821..18894)
                     /locus_tag="VMZ53_05770"
     CDS             complement(17821..18894)
                     /locus_tag="VMZ53_05770"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HUS27992.1"
                     /translation="MRIADFVTLPARYELSQQKALEWMAAAHAESEATLGGLDLLAKR
                     AFETKMLRVLSKVACTPDKIAQRGHHVDHVARGAFDPMPRGAGTFARMKTYATIVDDA
                     FMRLYSETAPPDDIIHVTCTGYVSPSGAQKLVAAKKWPTRVTHAYHMGCYAAIPALRI
                     AAGAGGKRVDLVHTELCSLHMDAADHSPEQLVVQSLFADGLIRYSLVNEGPGLELLTT
                     HERVLPDSAHAMSWRVGDHGMAMTLARDVPARIGVVLRDFVSELFARAKLPLSDLKRC
                     AWAVHPGGPKILDGVRDTLELGESQIATSRAVLREHGNMSSATLPYIWMRLLRDVPNG
                     TLIPSLAFGPGLTICGALLEKRC"
     gene            18994..>19437
                     /locus_tag="VMZ53_05775"
     CDS             18994..>19437
                     /locus_tag="VMZ53_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUS27993.1"
                     /translation="MSSRLAAMTIAILGTGLLGSGFARALRKKGEPVRVWNRTIERAR
                     PLAADGIEVADTAEAAVRGASRVHVVVSDDAAVDSVLEAASPGLARGTFVIDHSTTST
                     AGTIRRTRDWNGKGIRYQHAPVFMGPQNALESTGLMLISGEPAVID"
BASE COUNT         3282 a         6105 c         6566 g         3484 t
ORIGIN      
        1 ggcgtgccac gcggactcga gcagcggggc gaaatcgtcg atctcgagtg cggcgtcgtc
       61 ccacacggtg atgagctggt cggcggcgcg ctgcttcgtg cggcgcgaga cgtcgggctt
      121 gcggagcagc gcgaggaaca gctcttcggc gaggagcgtg taaatcgatt gggcgacttc
      181 ttcgcgcagc tcgtgcgtca tcttcacggc ggtcggcacg tcgcgcagcg cgcttgtcgc
      241 tttgacgaag ccgcggagga agttgatctt cgtgagcagg tgcgtgcgag agagcgtcgc
      301 cttgagtgga aggacgaggt cgcccgagag gccgtcgaga tcgcagaggt gatcgatgag
      361 cgtttgctcg tcgcgctgct caccaccgag ctgggtcggg cggcgcagcg ggcgctggcg
      421 atcgagaagc tcggacgtgg cgcgcagctc ggagatcaag ccttgcagga tcaccgcgtc
      481 gtttggcgag cgcgaggcga ccgcttccat tgcctgctgg acgagctcgg actcgtggcg
      541 atcgagcacc gacaccgcga atgcctgcgt cgcttgcgag gtgaggggcg gcgcggacgt
      601 ccccgatgcc gtcgccaacg acgtcatcgg tgggttcgtg ggggtacgtc cggggaacgg
      661 cacgggtaga aagcctgcga aggtagtagc tcagcttgac gcaaaatgat caatcggcat
      721 tgttctcagc ctgccctcgc tcacgcctcc gccgcagata ccacctgacc gcgatcgcca
      781 tcacggctac cacgactacg acgccgatga cgagatcgaa tcgttttaac ttgttggcga
      841 cgctttccca gttcttaccc aggtagtacc caagcaagat caaaaaggat ccccagaggg
      901 tcgagcccaa gaacgtttgg agcaggaacg accgaaacgg catcctggcc actccggcag
      961 gaagcgagat gatgtggcgg atcacaggca cgaggcgggc gatgaagatc gtcgccttcc
     1021 cgtggttcgc gaaccacttt tcggtcttct cgatgtcgtg ctttcggatc aggaggaact
     1081 tgccgtactt gagcagcagc ggcttaccgc ccgcggcgcc gatccagtag ccgatgctcg
     1141 agccggcgag cgccgccacg ctgttgatca cgagcagcac gtaaatgttg aagacgcctt
     1201 tggaagccat gtacccggcg aacgggaaca ccagctcgga aggcacgggc acgagcgtgc
     1261 tctcgagcat gacgagcaag aagacgcccg ggtagcccat gcgggccacg atgtcctgaa
     1321 gaaactcggc gaacgcgtgc atcgggtacc gctgcttccg ctaggtcgac gtgaggaacg
     1381 agaagtaacg ctcggggaag tcgttgaagg ccatcgccgc accgcactcg gggcacgtca
     1441 tcgtgggggg ctggagctgc gcgagcccgg cggcattcgc gactgcatcg atgagcagtg
     1501 actcctcgcg gccgcatgcg tcgcacgcga acggcgcgaa gaacgaggtg accttcgccg
     1561 tgccgcgcgt cgcgatgatc atgttgagct gatggatcag cacctccgcc acgcgccgca
     1621 tctcgatcgc gatgccttgc tgcgtggcag acgcctgcat gcggatccag tcgcgcacgc
     1681 cgagcgagtt gatgaacgtg atgccgccga ggtcgagcac gagcttgccg ccgcgcgcgc
     1741 gtccgagcag cgccatgagc tcggcatgct cgtcgatcgc gccggtgaga aggagcgcgt
     1801 cggcggactc ttgcacgtgc atgcgaggcg taggcggcgt atcggtcacg ggcgctccac
     1861 gaacacgttg tacgacgaaa cgggcgcgcg ttcgctcggc ggatagcgaa catcgatgag
     1921 ggcgatgatt tcggtccgct tgccggggct caaattgaac acgaggtggt cgcacgaccg
     1981 gtagctgagc gagatgccca tgccggcgcc ggcgccggac tcggtgaccg cattcttcgc
     2041 cagcgacctc aagatcgtgt cgcgatcgag actgccgaac tggtcgttca cctcgagcgc
     2101 gagatagcgc gcgtcacaac cccagcgcaa ctgcacggca tgccgttcgt cgagctcgat
     2161 ctcgacattg cgcggcgtgt ccttgcgtgg gtggtggcct tgcacgtcga ccggcgcgtt
     2221 gtgcaccgca ttcgagatca gctcgtcggc aacgagccgc gccatcgatg caacgcgcgc
     2281 gctctgcccg cgagcacgga cgtcgttcgc gagctcctcg acgattcgcg accgctcgct
     2341 cgccttcgtg atcttctgcg agtgcagctc ggtgccccac aatagatatt tctcggcgcc
     2401 gaacaggtcg ccgcgcacga gcttctgcac ggtgacggtg agctcctcgc cgagcaccgg
     2461 catcggatgc acgagcaggt tcgtcacggc gccgaggcca agaatgtgcg gcacgtgatc
     2521 gggcgcgctc tcgccgagca gcgtcatgca tgcctctgcg cccgccgcac gcgcttgcgc
     2581 caagaacgct tcgccgtcgg cattcatcgc gatattgccc gcgacgacaa cgacgagcgg
     2641 cggcgagtgc tgcatgagcc gcgttgcttg ctcgagcgtg tccgcgatct cgatgcggtg
     2701 gccggtaatc cccaggatgc gttgcaccgt ccgctgcgtc ttgcggtcgg ggtgaacgat
     2761 gaggatcgtg tgcacgatca gattccttta ccaccgcggc ggttgcgcca cacgagaagt
     2821 gcgcagaaga ctgagcccat cagcgcactg atgaacagga tgacgaacac cacgctgagg
     2881 tcgtcctcct tgtagaagcg cgggatgatc acttcctgga cgatcgggcc gagcgcgccg
     2941 agcatcgcga tcgtcgcggt ggcgaacatg gccgtcttgc ggctcccgat gtccatcgca
     3001 cctgcgccag agagcaacgc gtcggggccg tagaggaaga agccgacgag accgagaagc
     3061 agacagaaga cgacgacgct ggtgttgccg aacatgacga gcatcagcgt gacgaccgac
     3121 atgcagagca tcatgatgag cgcgatgccg ccgcgtttgc tcttgaagaa tcgatcagag
     3181 agaaaaccgg tcacccagat gcccggaatg cccgctatgt cgaacagcac cgaatagatc
     3241 gcagcatcct ttccggacag cttgtagttg ctggacagga agtacgcaga ccacgaccag
     3301 accgcgtagc ggatcagctt cgagaagaag tagaagccgc caacgatgag gatgttcgtc
     3361 cactgatcgc gcgtgaggtt ctcggcgggc tgctccctcg attcgtcgac agccgtgacg
     3421 ggatcgtcga ttgccggcag cccgacatct tccggctggt tgcgctgaaa gtaatagaac
     3481 aggacccaga tcgcggcgag cacgacggca cccacgaaga acgttgcctg ccagtcctgt
     3541 tcggcgggac tgacgtcgcc gagcaagtag ccgagcgcaa acgtcgcgct gatcgaaccc
     3601 acgacgaagt tcgtacccca caggcccatg acgtgaccgc gctcgtgctt gtggaaccaa
     3661 ttcgccatcg tgccgacatt gctcgaccaa cccgttgctt gcgcgacgcc attgaccgcg
     3721 atcagaccag cgagaatcca cggcgagagg ggtatgccca tgatcaacgt gacgagcacc
     3781 gacagcgcca tgccgatgag caggttgttc cgcggcccga gcttcgtgcc gatccgcgac
     3841 gagagaaact ggccgatcgc gtacgcgatg aggtacgcgg ccatgatgtt tccgacggtc
     3901 actgccttgt gccagtggtt ctgcgtctcc atcgccttct ttgcgacgga gaatggcttg
     3961 cggcagaagt aaaagccgac gtacgagagc cacgtcgcga cgaacacgcg agctcgccag
     4021 acgcgatgat cggccgtcag cgccgttgcc attcagcgac cgaggatacg ctgatacgcc
     4081 gtgccgagtg cttcggccag catggcaaga tccgccgctg cgacttggaa atcttcggac
     4141 gtgtcgatcg gatcgccgac gacgagaacg gccgccgcct ggcctttgac catcacgggt
     4201 gcggcggctg gatgcgcggc accttcgagc gcgcgcgtca gctgatcttg cgtgggaccc
     4261 gcgggcgctt ggatcgagac gaagcgggaa tcgatcgcgt gctgcagcgt cgatggagct
     4321 ccgagcggca tcgtgatcga ttccggtgcg atgacattgt ggccgcgata gccgacggca
     4381 gtgcggtcgc gaatcgcgaa gacgacggcg gtctgccacc gcgtgccgac atacgcgagc
     4441 acgagatcgg tggcggcttc gcgcgacgtc gcgtgctcga gaccgacctg catcatgccg
     4501 actgacatct tgcgcgtcgc agctggatcc gtgcgtgcgc gcggatgacc gcgaggcggc
     4561 gttgacgcag cggcgaggcc gacgattgga atctggcccg actgcgttgg atctttttcg
     4621 acgcgatcgt cgtcgaggtc tgtcagcgac aggcgaatgc tctctggatc gagggcggcc
     4681 atgtcgggca gcggcggcgg cggggcgggc gtgggcgagg gcttcgccgg cgtgatcggg
     4741 ttgtcttcca cctgcgacga gaaatcgatg tcgaactcgt cgttcggcgc gtcgccgtag
     4801 gattcgcgga cgaggttctc gagccgcgat gccggcgtga tgaccatcgt gacgtcggtc
     4861 ttgagcgcgc gttgcagctg ggcgagcagg gccggtgccg ggtcgcgcac gcatacaatg
     4921 atgtggccgt tgctcgcgcg gccgatcggc aacgcccacg cactgcggcc gagctcggcg
     4981 gggatgagct tcgcgagctc ggggtcgcgg ccggcgaggt gcttcgcgag cgcgcatgga
     5041 acgcggcgtt gttcgccgag tgcgcgagct gcatcgtcga aatcgacgag gcccttcgcg
     5101 atcagatacg agacgaggcg cttgtcgctg ggcttgtccg ccaaggcccg ggtcagctcg
     5161 ctctggcgca acttgcgttg ctcgacaagc agctcgccga ttcgcatcga gtcgccatct
     5221 tgccataaga cgcatgtagt cgtaggaacg ccacgtgctc gagcttgcgg tcctcgcgat
     5281 gttggtcggc gttgccggca cggcagcgct tgcgggactc gccggtgcgt tgcccgacga
     5341 gcgcaaggta aagcgcgaga tcgcgacgca gcgggtgcgg ccaatccgaa cgctcgtcga
     5401 tggcgccaat gtcgcaatcc gcggcgaggt cgctcccgcg cgcgaaggcc tgacgaaggc
     5461 gccactcacg ggacgcgcgt gtgtgtattg ggtgctgcag gacggccggc gcaggaaggt
     5521 tggcggccat ccgttcgtgt tgcgcgattc gacgggcgag acgcgcgtgg tgccgatcga
     5581 tgcgcggctc gcggtgccgc cggtgcatca acaagtcgac gacggcctcg tcgaatatgc
     5641 cgtgttgccc ggcgcgactg tcaccgtcgt cggcgtcgtc acgtacgaac cagatccgga
     5701 cgccgtcgcc gcggtcaccg gttaccgaaa agagctaccg acgcgcccgg tggtcagcgg
     5761 aacgcgccgg cagcgactac tgattggcta gtaaacgacg ccactagcgg ggcagaaccg
     5821 accgcgtgca ctctcgtcat cgccgatcgg ggtcgccgag aggaagacct cgtcgaggct
     5881 tccgccgtag gcgccaccga ggcggaccga gtcgagagct ggagcggtgc ccggtcgcgc
     5941 gtcgccgatt tcggtgcgaa cgccgtcgac ccagaggcga agcgtcgcgt cgctcagcga
     6001 tgcgatcacg tggtgccacg cgcctgcggc aacgggtgcc gaggtgacgg tatgcgtcgc
     6061 gctggcgttg tcgatgaccg ctgcgctgaa cgtgacgttg cctcccgcgc cggtgacgcg
     6121 caccgcgatg cggccatcac cgctgagcag gatgtcgcgc gtgccggaca gcgaagccgg
     6181 cgaagcccac agctcgatcg tgaagttgct cgtcgagcct gcggccagcg gccacgcgac
     6241 caggtcacct tgattcgcgt cgaacgcgag gccaccagaa aagcggccgg acgtgaacat
     6301 cgggtcgatc gcctctgctt gatcggtgtt gccgagcgtc gccgacgcac cgccggtcgc
     6361 gttcgcgagc gtctggccgt tgtcgtcgag atggagcagg ccgagcgagc ccgccggatc
     6421 cggcgcagag cacgtgatgg tgaacgaccg cgagatcgtc gtcatgttgc ccgcggcatc
     6481 cgtcgcgttg atcgtgaacg attggttgcc ggcggcagag ttgaagccga acgggctcgt
     6541 gcacggccgc gcggcggcac cgccgacgga gcacgtcacg gtgccttccg acgcgctgaa
     6601 cgtgtagacg acgcgcggtc cgacggtgtc accgttgccg gggccacccg tgatcatgat
     6661 gttcggcgcg acggtatcga cggtccacgt cgcggtcgca gccgaactgt ccgtgttacc
     6721 ggcggcgtcg gtcgcgcgaa ccgagaacgt gtgcgcgcct tggccgagat tcatcgcgtt
     6781 gtacggcgac gtgcagttgg ccatcgcggt gccatcgaga ctgcactcga acgtgacgtc
     6841 attctcgttc gccgtgaacg agatggccgc gctcgtcatc gagatcgagc cgctcggtgc
     6901 cgctgtgatc atcgtctccg gcggagtggc gtcgatgatc cacgtgcggg tcgccggcga
     6961 aggatcgtaa ttgccgacgg cgtcgcgcac gcgcacttgg aacgtgtgct gccccatcga
     7021 caggttcgag tagccgtgcg gcgaaacgca ggttgcgaac gcgccgccgt cgagcgagca
     7081 ctcgaacgtc gcgcctgtgc cggcatcggg cgaaacaaac gtgaacgtcg cgctcgtcga
     7141 attggacgcg ccgacgggac cgtcgatgag cgttgtatcc ggcgtgctgg tatcgaccga
     7201 ccacgcgtgg atcgcgggcg acgaatcgac gttgcctgcg cgatcctttg cgcggactgc
     7261 aaacgcatgc gcgccgtcgc tgatcgggcc gaactggtcg ccgctgcggc acggcgcgaa
     7321 cgcgccatta tcgatcgagc actcgaacga gacgttcatc tcgcccgagg tgaagcggaa
     7381 cgtgaccatc gtgctgttgt cggctaccgc cggcgcctcg gtgatcatcg tcgacggtgc
     7441 gacggtatcg atcgaccaca ggtgttcggc gggactgtcg tccgtgttgc cgctcgtgtc
     7501 agtcgcgcga acgctgaaca tgtgcgaccc gtccggcagc gagcgggaga agggcgacgt
     7561 gcacggctcc ggcgcgccgt tatcgagcgt gcactggaac gtcgcgctcg gcacgttcgc
     7621 tttgaacgtg aacgtcgcta cagacgacga cgagaagtcg gagggtgcgt cggtgatctc
     7681 ggtctggagc tcaccggtgc cttcttcgcc tgatccgttg tcatcgccga gaggttgctt
     7741 gtccggaatg ctgcacgccg cggtcgcgag cagtgcaatg aggatcttcc gcattagaac
     7801 ctcctcgaga gcgtcgcgcc cacgtggtca gagcccagcg acggtgcaac gaccgtctcg
     7861 gtgtccggct tgccgacgaa ccacatcacc gtggccgcag cgaccgctgc accgccaccg
     7921 atcaagagga tgttggcgtt gcgcgcggac gtgttcgcct cgcgattgag gcggaggcct
     7981 tcgtcatcgc cgcagagcgt ctcggggcag atcgcgtcgg atttcgcctc gagcgtctga
     8041 gcatgcttgc cgaagtagac gccgccggcg agcgcacctg caccgagaac cgcgaagcca
     8101 acggcaactc cgcgcgtcgt gctccacgtc ttctcgacga caaccgtctt cggacgcgtg
     8161 accacgaccg tcgacggctc gctgaccggc gcggtgacca cgcggggagg cggagtgaca
     8221 ggcggcggga cgggcttggc ttcggcgacg ggctcgagcg acggcacgac gacccagcgc
     8281 ttgccgccct tgccgacggc gacatcggtc ttccacggct tcgcgttcgg cgcttccgct
     8341 acgatcgtgt gctggcccgg atcgacaacg atgccggttc cgtacacggc gggatcgagc
     8401 gtctcgccat cgcgcttgat gaccagaccg tcgagcttgc tcttcggtcc aacctgaatc
     8461 gtgatcgtcg cgagcgcttc ctcgagcttg tcggcgcgct tcttcgcttc gagctgacgc
     8521 ttcttgtcgt tgccggcgag cttggccatc gactccgcct tgcggaaggc ggcccacgcc
     8581 gtcgcgacgc ggccttgctt ctcgcggcag ttgccgagat tgagcaacgt gccgacgctc
     8641 gactcgagct tctcactcgc ttcgagcttg tcgcagcctg cgtccaactt gccttgcttg
     8701 atgagagtgc ggccgtcccg gaacagcgct tcggcacctg ccgattgcgc gtgcgcggcg
     8761 gacggcagca tcaatccggc cgcgaaggcc agagctactg ctctttcaac gtgcgtcata
     8821 gaggtcctcg tcgtcctgtt gcgtcgtgct cgccggcacg tgacgccgcg gttgaggcgg
     8881 cctttgcacg tacgggcgcg gaggtcgtgt cactttcgcg gggcgctgaa ccgttacttc
     8941 tttcggcgct ggagcttgtt tcggcgctgg agcttcttct tcgtcgggca agctcgccac
     9001 gagatcttcg agctcgggcg gagcggtttc gtgagcaacg atgccgcccg ccgcaacgcg
     9061 aggctcttcg gtcgtcagtg cgagaggcat cggcggctcg gacgccggcc gcttgtcgtc
     9121 gagcgcgtag ctcgtcgcgt tcagaccgcc gacgatgccg atgaacagcg ccgccgcgaa
     9181 catgaaggtg cgaccgaggc ggcgacgcgg aggaggtggc gcagcttccc actcgtcgct
     9241 gatcgacgac gcaggcaccg acatcgaatc gacaaaccag ctcgcggcag acggcgtgac
     9301 ctcggcggcg acttggcggc cttcattgac gtcgtactgc gagtggaatc ccgtcgcgag
     9361 cggcgtttgg ttcatcgcgg ccgccgtcag tcgcgcaccg tgatcgctga tctcggcgag
     9421 cgcgcgttcg atggcgccgt gcagctcctt catcgactgg aaccgattcg ccggatcctt
     9481 cgcgaggcag cgcatgacga gcgcgtcgac ggaaggcgga atcgtcgggt cgatctcgct
     9541 cggcgttgcc gcgtcctctt tgaggtgtgc agcgatgaac tcgccgacgc tttcgcagtt
     9601 gaacggcggc tgcccggtca gcatgtgata gagcgcgcag ccgaccgaat agatgtccgt
     9661 gcggtgatcg accttgcctg cgccgcgaca ctgctccggc gacatgtaca cgggcgtgcc
     9721 gagcatctga cccgtctgcg tgacgctgcc gtcttcagac gcgccatcgc cgagcttgca
     9781 gataccgaag tcgagaatct tcgtgcgctc gccgctctgc gcttctgcat cgcggatgat
     9841 gaagacgttg ccgggcttga gatcgcgatg aacgatgccg agcgcgtgcg ccgaagcgag
     9901 cgacgccgtg atctgtcgcg cgatgcggag cgcgtcgccg atcgtgagct gatgcaggcg
     9961 ctctagacgc gtcgacaacg actcgccttc gagcatctcc atgacgatgt atgcggtgcc
    10021 atcgacgtgg tagccgaagt cgaagatctg gactacgccc ggatcgctga tcgccgagat
    10081 cgcttttgct tcgttgaaga agcgatcgac gatctcgtga tggctcgata gtgcgggcat
    10141 cagcgttttg atcgccgcgc gacggccgag caggatgtgc tcgccgaggt acacggttcc
    10201 cattccgccc gcgccaagcg tccggaggat cttgtactga ccgatgtggc tctcgacgtt
    10261 cacggttgaa caaggtctag cgtgcctccg tgttttgtct aataactccg cgcagttgag
    10321 gcacattggc acggtgtgct cctactgctg tggcggagct gtcagcgcgc tgggtaagcc
    10381 gccgaaattc cgctctgcac ggcccccaga atcggggtgt cgagcgtgtg tcccgtgtcc
    10441 cgttcgagct cacgcggatc gtgagaacag acaacctgga cctcgactgc gtgatcccga
    10501 acgagctggc gcagccgctc ttggttcgcg agtctctgct ctcggtcctt ctccatcatc
    10561 cactggtagg cgcgcagtcc cggcgcacat cgcggttgcg aaggatgcat ctcgtcctga
    10621 tcgaagtacg cgtcacctgc cagcaagagc caacgcccgc tgtcttcccg cactgcgatg
    10681 cccgcgtggc ccatcgtgtg accgacgagc ggcaccatca ggacttcggg cggcaatccc
    10741 ggaatgtctc gcacgcatga aaatccgtac cagcgctcgc ccgccgtcga gtcgtatgtc
    10801 tgccagcgat cgtacgagcc ccactgctga gggcgatagc gttgacgatc gagccagctt
    10861 cgctggcgga ccgcatcgct cgcctcgtcg tgaagcatgt gcaccgtcgc ttccggaaag
    10921 tcgtcgagcc ctcccgcgtg atcgaaatcg aggtgcgtga gcacgatgtg ccgcacgtcc
    10981 tcggcgtgat agcccattgc ttcgatctga cgaatcgccg tgagctcctc gcgaaagtcc
    11041 ggatcgagca agctcagaaa gaatcggctg agccgctggg tcgggcgcgc gcaatccagc
    11101 aatccgaagc cggtgtcgag cagaacgagg cctcgatcgg tctcgatcac gatgcagtga
    11161 cacgcgagct ttccgcgcag ccccgtcgag cgccggtcca tgagagcgcc accgagcggg
    11221 cacgacgaga tgcagttgat gtgatgcagg cgcatgctgg ctcatcgagc aacatgcatg
    11281 cgcgtaggtc tacgaggagc gccgatcagt ggaccgcatc atcgaggcgc ttctcgagcc
    11341 gctgaaatga atcttcgacg ttcgattgga actcgtccca cgccggcttt gcttgttcgc
    11401 gcgcgctctc gagccgcacc gcaagctcct cgcgatcttt gcgcagctct gcggcggtct
    11461 ggtgcgccga ttcactcgcg gactgctcga gctcgttgat tcgtgcatcg agcttcgcga
    11521 gccgttcacg cgatgaggtc tcgaagctgg agcgaactcg aacgtactcg tgatcggtgt
    11581 cccgcacgcg cgcgtgatcg tcgtcgggga cgatggcggt gtcgcgatcg gcgggtgtgg
    11641 tctcgacggg ttttggcgcc ggtgcaggcg cgcggttgtc gatgtcccga ctgatgtcgc
    11701 cgcggcaggc agcggtggca agcaaggcga gcaatacggt gcgcatagca attcctcctc
    11761 ctcggagaaa tgcattggtg gtgccttgcg aaagattcgc agcgcacgcc taacgaaacc
    11821 gcgtttacag tttcctcgac gagaaacgat ttcgtacgag aaaccgcgcg agcgctggcg
    11881 tcacacgttt agcatcctcg catggaccca cgcgcgctcg ttcgcgctgc ggtcgctggg
    11941 ggtttgctga tcatttcgat cagtgccagc ggctgtggct caccgcaaac cgccgacgaa
    12001 accgatgcca atccaggccc gaacttctcg actgacggag gcggggtcgg ctcggatccg
    12061 ggaacgcccg agaacccacc agcgccaaag acgttcgacg tcgcgctcgt tcccgacggt
    12121 gcgagtggca cgcagcgcgt gaactttgca gtgccggttg caccgggcgt gatcgccgac
    12181 gcgtcgctga tcaagatcac cgcaggcggc ttcgagctgc cggccgcgcg gcgcgcgctc
    12241 gcgaaatacg ccgatggcag tgtgcgcagc gtgcagcttc aagtcgacgt cgacgtgaca
    12301 gcgacgcatg cgctgacggt ggagctcggc gctgctggca tgacggggcc gtctctcgcc
    12361 gccgtctcga cgacaacgac aggtaccggc aacaacgtgc atcccaaagt ctgggcgctg
    12421 ttgccgacgt cggtgctgat cggcagtggc ctcggcggga ctctcgtcgc gcgcgctgat
    12481 cagacgatcg atgcgtggga cgcgatctgc gactacgaca aatgggacac ggctgcattc
    12541 ctcgtcaatg cgtcggcgag ccgcgatgtc tggctctacg accgcgtgac ggcgatgtat
    12601 cgcggctact tcgttaccgg cacgatctcg ccgctgcgtt ccgcgtatcg cgaggccgag
    12661 atctatcgcg ctggcatggt gatcacgaat ggcgtcgcga cggcgattgc accgccgact
    12721 gccaacgacg acctcaagta ctactacgcg cagaacatgg cgatgcacta cttgctcacc
    12781 ggcgacgatc gcttccgcga agcggccgaa gccgtgagcg cgcgcatcgc ctcgatgtgg
    12841 aacccgcagt acaacggcgc tgatcggttc tggacggagc ggcacgcggg cttcacactc
    12901 ctcgcacatg aatgggcgct gatggtcacc gacgatcagg cacaaacgat cgcggcgcgc
    12961 tcggaggccg ccgtcaccgc gtacctcgcg acgcagatga cctatccgac gggctacacg
    13021 gatcagagtg cgcgttgctt cggccactcc gccaccgcgc acggcgaggg ctacggctac
    13081 aacggctgca gcccgtggat gagcgcgatc ctcgctgacg cgctcgatca gtacgcacgt
    13141 cgaatcggtg gccaacgcgc cgtcgaagtc cgcgcgtcgc tggcgatgct cggtcgctcg
    13201 ctggctcgcg atggccgcga ttcgacaggc aagccgttct actggatggg cgaagggacg
    13261 acgcaggacg aagtcgacga ctacgacgag cactggggcg aatccgcgta cgtcatcgcg
    13321 ctcgcgtgga acgcgactgg gcgcaccgag gcgtcgctca agaccgccgc agatcagctc
    13381 gtcgctgggc tcgaatccga cggcgtcgtc ggtcaggtgc gcagcttcaa ctggcagtgc
    13441 cgcagcgcga tcgcgacgcc cgcgctcctg aagtaatcac ggcgcggtgc cgctctcgaa
    13501 cgcggcgcgg aagtgcgcga ggaacgcgtc gtacacggcc tgatcctcga ggcgcgagaa
    13561 cagctcgtcg ttcgcgtagt tcgccgactt cgtccagttg tgcgagccgg tgaacacgat
    13621 cgtgcggcgc gcggtcgagt ccgcgaactt cgcgtcgacg aggacgtact tgtcgtggag
    13681 cttggcgtgc ttgacgctga tgttcgcggc tttgaggcgc gcgagcgatt gcgactggat
    13741 gtggccggtc gcgacgcgca cgaggcagcc gcctttcttg acgcggatga gctggtcgat
    13801 gaggtcaggg cgcgagtcga agatcattgc ctgcgcggcg cggacctcgc agctcgcgcc
    13861 ggggacgatg tcgttgaggc gattcttgat gaggtcgctg tcctgctcgg gcgacaggta
    13921 gatgcgcgtc gtcggcgaga cgacgaagcc gcgatcggca tcggcgttgt agtagtcgtt
    13981 gttcgcgaag tgcttctgct ggaacagctg catgaagtac ttgtcgagct tcgtgtagag
    14041 ctcggtatcg ccgtagatcg tgatcgtgtt gttgaacgtg ttctcgcccg tggcgtgcgt
    14101 catgttcgct gagccgaacc acgtgacatt cgtgcgcagc gtgccgtcgg gcgccgcggt
    14161 cttcgagaac gtcatcagct tcgtgtgcat gatgccgctg ctgtccgtcg tgatgcagcc
    14221 gaagttgccg ttcgcgcgac ggtcgccgca gaagacgtgg ttcgcgccga gcgcggcagc
    14281 gagctgacga ggcgaatcgt cggcgtcgaa ttcgtcggag ccgtcttccg cgacgagcac
    14341 cgtcactccg cgctgttttg ctgccacgag cgcggtgacg acgttgttga ggacgaggct
    14401 gtggatcgcc gcatggatcg tctcgccttt cggcgtcgcg tcgatcaggc ggatcgtctc
    14461 gcggatgatg ttcgtgtcgt ggccggcgtg ggcggcgggg ttcgtgaagt ggacccaacc
    14521 cgggatgcca ccgatcgaga acggcgtgcc gaccgcgggg gcggggcttg cgtccgcgag
    14581 cgcatcggca tcgacttccg agccgtcgtc gtctggacct tccgattccg ggtcggcagg
    14641 cgcggctccc acacaagcgg caaaaccaag cacgaggaca gcgaagtggc gcatcgatgc
    14701 ggggcggcat gtgcagccgt cgtgcccgca gagcgccgag caaagtcgct ggcttcggcg
    14761 cgcggaaccg gtggacatgg cgccgcgaga ctgcaaacag ccgtccgcgc gcgagcttcc
    14821 tcagtctttg tgcttcttcg gcttcttgtg cttctcgggc tttggctctt tgtgcttctc
    14881 gacgtagacc ggctggcctc gctgggccgg cgggcgccgc gtcgtcacga tgcaggccgt
    14941 agccacgagc gcgaccaatg acatcgcgat gatgagtgaa gcgtgcttgc gcagcatggc
    15001 tgcgaacata tcggatgctg gtcgcggcta caatcgaacc cgtggattgg agggctctcc
    15061 gtcgctggct tctcgccggc gtcgcggtcg tcgctgtgct cgggctcgcg gcggagctcg
    15121 cgcatgcgta tgcgccgacg gatctcggcg aagccctgct gccgaagctc tcgctctcct
    15181 acgagcagaa tttgccaact tggttctcgt cgtcgctctt gctgctctgc gcaattgcag
    15241 ccggcgcaat tgcttcgcgg ccaccgccga tgcgcctgca ctggtggggc atggccgtgg
    15301 tcgcaggctg gatgtccctc gatgaagcgg ccgagatcca cgagcaccta gcggggcact
    15361 tcaacacgca cggcgtcctg tacttcgact gggtgatccc cgcgaccgcg atcgtcatcg
    15421 ccctcgcgct tgtctacgtg ccgttcgtgc ggcgactcga atccgcgacg cgcacgcggc
    15481 tcatcatcgc agcgaccatc tacgtgggcg gcgcgctgat gatggagctg ccacttggct
    15541 ggatcaccga gagccgcggc atcgagaatc tcgcgtacgc gctcgtcgac tgggtcgaag
    15601 agacgatgga gatgatcggc gcggcgctcg cctttgtcgc gctggtcgcg catcgcaaca
    15661 gcggcgtcca gcgatgaagc cgcgcagccc attcatcgtc agcttcacgt acgactggat
    15721 cttcttcctc gccgcaccga tgctcgcgct tggtctcggc atcctcgtgt cggatcgtcc
    15781 ggcgctgtcg gtcagcgagt attcgttcgg cggcgagcgc gacaacctcg tcggcttctt
    15841 caccggcacg atcgttcacg cgcatctcgt cgcggtcttg ttccgcagcc acggcaacgc
    15901 ctcgatccgc cggctctatc cgattcgctt cctcgtcgtg cctgtcgtgc tgtggctcgc
    15961 gatcgtcgtg tcgccctgga tcgcggtcgc gagcaccgtg gtggcgacgt tctgggatgt
    16021 ctggcactcg ggcgcgcaga cgttcggctt cgcgcggatc tacgagcgca atgcgggtac
    16081 gccgcccgag ctcggccgcc gcctcgactt cgtgctgcag caggtcctgt atgccgggcc
    16141 ggtgctcgcg ggcgcgacgc tcgtcgatca cctcgactcg ttctcgaact tcgatcacat
    16201 cggctcgaca ctcttgtcgt cggtgccgac ttcgatcgag gcgcatcatc gcgagctcgc
    16261 gtacgcggtg atcggagtcg caacggttgt catcgcggtg tacgtgctcg tgtattggca
    16321 gctcgcgaag cgaggcgcgt ggtcaccgcc gtggctcaag atctggctcg tcgcgtcgac
    16381 ggggttcgtc tcgatctaca cgtggggctt caactcctgg ggccaggcat ttctgatcat
    16441 gaacctgttc cacgcggtgc agtacctcgc gctcgtgtgg gcgatggagg gcacgcgcat
    16501 cgccgggatg atgcgactgc cgaagcgcgc agcgttctgc gtctacttcg gcggcgtcgt
    16561 cgtgtatggc ctcggcgtgc aggcactcga tccagacatc acgacgctat gggcgatcac
    16621 catggtcgtg tcgctcatgc acttctggta cgacgcgttc gtgtggagcg tccgtcgcgc
    16681 tcaggtctag gcggcgcgca aaaccggtgt aaccgatcgc ggtcgacttc gtgaacgtga
    16741 acgcgaagtc attgccgacg cggccggtga gcagcatgcg gaagagcggc cagcgaaagc
    16801 gcggacgcga gccgtggagc gtcgcgatct cgaggccgtg tgcctggcac atcgtggaga
    16861 cctcggcggg cgagcgaaat agatcgatca cgtggaggtc attcggcgtg ttcttcacga
    16921 accactcgac acctttgatg acgatcaggt gcgcgatctt cgtccggttg aacgtgtgaa
    16981 agaagaacac gccgccgggc ctcagcacgc gagcgacttc cgcgatcagc cgatccggtt
    17041 ccggcacgtg ctcgatgagg tccatcgcgc agacgacatc gaagctcgcg gcagcgtagg
    17101 gcagggcgca ggcatcagcg acgtcgtatg tcgcggttcc atgtgctcgg gcgacggcga
    17161 gattctcggc ggtcgtgtcg atgccggtga cgacgtggcc gcgcgcggcg aggaaattcg
    17221 cgaggaagcc gccaccgcag ccgaggtcga gcacgcgctg cgacgccggc ccgagcgcgc
    17281 gcacgatctc gtcggcgatc cacggattgc gctggcgcga ctccgcacgc agtagcgcga
    17341 ttggcgtgtc ctccgcgtga taccagcgct cgccgagatc ggcgtaccaa tcgttgttga
    17401 tcgtccgcgt gcggcgcggc aggttccagt agacgatctg atacgtcatg aacgcgatcg
    17461 tgacggcgag ctgcccagtg aggatgccgc gctcgccgag gtgcgcgagg tagccgaatg
    17521 ccgcgagcac gatcgggtgc agcgcgaaca gcactgcgtg cagccattgc tcaccggcgc
    17581 cgcagagctt ggcgtgcacg ccttcgtctt tggtgacgaa caacgtggat gcgatcgcga
    17641 gcacgatgta gccagtgagg ccgccgccag cgagcagcca cccgaggcac gcgacaattg
    17701 tcagcgtgtc gagcggatgg ccgatgcgct cccagcgcgg catctcgcga cggcgatgaa
    17761 acacgccttc gtcgacgatc atcgcgaggg cctgcaggcc gatcggaacg aggcaggcgg
    17821 ctagcatcgc ttctcgagga gagcgccgca gatggtgagg cccggaccga acgcgaggct
    17881 cggaatgagc gtgccgttcg ggacatcgcg cagcaggcgc atccagatat acggcagcgt
    17941 cgccgatgac atattgccgt gctcgcgcag caccgcgcga ctcgtcgcga tctgcgattc
    18001 gccgagctcg agcgtgtcgc gcacgccatc gaggatcttg ggtccgccgg gatgcacggc
    18061 ccacgcgcag cgcttgagat cactgagcgg cagcttcgcg cgcgcgaaca gctcgctgac
    18121 gaagtcgcgc aggacaacgc cgatgcgcgc aggaacatca cgcgcgagcg tcatcgccat
    18181 gccgtgatcg ccgacgcgcc aactcatcgc atgcgccgaa tcaggcagca cgcgctcgtg
    18241 cgttgtcagc agctcgaggc cgggaccctc gttgacgagc gagtagcgga tgagaccgtc
    18301 tgcaaacaag ctctgcacga cgagctgttc gggcgagtgg tccgcggcat ccatgtgcag
    18361 cgagcacagc tcggtgtgga cgagatcgac gcgcttacca cctgcgcccg cggcaatgcg
    18421 cagcgcgggg atcgccgcgt agcagcccat gtgatacgcg tgcgtcacgc gcgtcggcca
    18481 cttcttcgcg gcgacgagct tctgcgcgcc actgggcgag acgtagccgg tacacgtgac
    18541 atgaatgatg tcgtcgggcg gcgctgtttc ggaatacagg cgcatgaatg cgtcgtcgac
    18601 aatcgtcgcg tatgtcttca tgcgcgcgaa ggtgccggca ccgcgaggca tgggatcgaa
    18661 cgcgcctctc gcgacgtgat cgacgtggtg accgcgctgc gcgatcttgt cgggcgtgca
    18721 tgcgaccttg ctgaggacgc gcaacatctt cgtctcgaat gcgcgtttcg cgagcaagtc
    18781 gaggccaccg agtgtcgcct ctgactccgc atgcgcggcg gccatccact ccaatgcttt
    18841 ttgctgggac agctcgtagc gagcaggaag cgtcacgaaa tcggcgattc gcacgcgcgc
    18901 gcgatctgca gtattcacgc ccgcgaatca cggccgctcc cgtcgggagt cgacgcgtag
    18961 tgtggcgccc gcgcactggc gagcgaatgg gctatatcga gtaggctcgc ggcgatgacg
    19021 atcgccatcc tcggtaccgg attgctcggc tctggctttg cccgcgcatt gcgcaagaag
    19081 ggcgaacccg ttcgcgtctg gaaccggacg atcgaacgcg caaggcctct cgctgccgac
    19141 ggaatcgagg tcgcggatac cgccgaggcg gccgttcgcg gtgcgtcacg cgtgcacgtc
    19201 gtcgtcagcg acgatgcagc ggtcgactcg gtgctcgaag ctgcttcgcc tggcctggca
    19261 cgaggcacgt tcgtgatcga tcattcgacg acatcgacgg cgggcaccat tcggcggacg
    19321 cgcgactgga atggcaaagg catccgctat cagcacgctc cggtcttcat ggggccgcaa
    19381 aacgcgctcg agtcgacggg cctgatgctg atctcgggtg agcccgccgt gatcgac
//
LOCUS       DATLBZ010000110        18561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9043657, whole genome shotgun sequence.
ACCESSION   DATLBZ010000110 DATLBZ010000000
VERSION     DATLBZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18561
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9043657"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VMZ53_05780"
     CDS             <1..296
                     /locus_tag="VMZ53_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS27994.1"
                     /translation="IEVDGKQVQLTTTQFELMALMIRRMAAEAHQPELVRGFVRSSEL
                     IADLSWDTREPSENHVKQLVRRVRRALVKSEIGDLIESRHRFGYRLRAIPKFG"
     gene            316..1251
                     /locus_tag="VMZ53_05785"
     CDS             316..1251
                     /locus_tag="VMZ53_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HUS27995.1"
                     /translation="MRRALGLSIVLAFPALAHAGDPKFEYGKAEEVKDVKDVEWVATA
                     EAGVVFTTGNSETTTATGGVKASRKTGKNKLALEASAAYAKSGARVLLDQNGNGMIDN
                     ASEIQDVQTITAETLASRLRYDRFLTELNSLFVAALASRDTPAGKELVLGAQAGYSRS
                     LRKTATSLTLGEFGYDYSHEDLVTVDVPSVSIHSARAFIGHHATMTEGADLDASLEVL
                     TNLNSENITTEDDNGNPYDGGPFQDTRVNAKISISAKIGQNLAFQSAIEVKYDRRPGP
                     LPVKNLAMGFTPAADTLDTIMKASFIYTFVAPTKK"
     gene            complement(1257..1532)
                     /locus_tag="VMZ53_05790"
     CDS             complement(1257..1532)
                     /locus_tag="VMZ53_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS27996.1"
                     /translation="MGDLIDDLETMRMVERFPDPDHGVIKRVRLGAKGRVWAAEVRRV
                     AAATEARWATQLGSKKMKSLRALLEELAATVEEQGTRTNGVHRSKRG"
     gene            complement(1878..5057)
                     /locus_tag="VMZ53_05795"
     CDS             complement(1878..5057)
                     /locus_tag="VMZ53_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUS27997.1"
                     /translation="MQWLANVCVRRPVFACVLILVLVVIGAVGYSRLGVDKFPKVDFP
                     LVTVVTPYQGASPGAVETDVTQKIEEAVNTVNGLETLTSVSTEGVSLVIAQFALEVSP
                     DKAAQDIQEHLNTVLRDLPPGVRPEVRKADPDAAPVVVLSVKGPAGMSTRELTRFADK
                     KVKQRIERLKGVGQIVILGGQERQINVELDPIRLAATGVSALEVQRAIAIANVNVPGG
                     QFQTGPKNMTLRIEGRAIEAKEIGEIVVRQQGEHAVRVNDVATLIDSEEDAETAAVRN
                     GTPAIALSVRKQTGTNTVAVVDAVKAAVEELNLPPGYSVDIVRDNSIQIRTSADQVLE
                     HLFVGALLAALVVLLFLGSLRSTIIAAIAIPVSIISTFGLMMIAGLTLNMMTLLALAL
                     AVGIVIDDAIVVLENIYRFIDEKRMKPFPAAIHATKEIGLAVLATTLSLMAVFLPVAF
                     MSGIVGRFLYSFGITMAFAIAVSMIVAFSLTPMMAARILPPPPPEGQERRRSALEKLS
                     DAIYQPIARVYSGILAFCLRHRWVVAIAIVGSCVGTVPMCKKVGGDFLPPNDEAQFEI
                     YVQMPEGSSLEATTLVAERLARKTRQIPEVDATLVTVAGSDQRQSNVANVYAHMTNPE
                     LRSRNQAEVMEEVRRKVLPDVPEGTRVAVQQVNDFSIGGQNAIVSYVISGPDLDKLEH
                     YGKTVLEKMKKVPGVVDLDSSLVDPIDETTLKPDLNRAAMLGVDPQDVTMTLSVLVGG
                     TEASTFEDRGDQYPVFLRAAERYRNDPNALALIGVPSRTLGTVPLVDVIQPSAGKAIS
                     KITRQSRERAVTITMNVSPGFNESAIVAELQKVIKELDLPPGYTAEPFGRSKEMAKMG
                     KAFLFAILLAFVFMYLVLAAQFESWVYPIIIMMALPLTVPFAVASLLITGGSLNIFSM
                     LGIIVLFAMVKKNGILQVDHANGLRRLGLPRTEAVLAASRDRLRPILMTTFAFVAGMV
                     PLITSQGIGSGFSKAMASVVVGGQTLSLLLTLVAVPVIYTWFDDLTRFFRWVWRKVFP
                     EDTDRGKAEAGIVDIHAVSQADRQQVNRH"
     gene            complement(5061..6122)
                     /locus_tag="VMZ53_05800"
     CDS             complement(5061..6122)
                     /locus_tag="VMZ53_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUS27998.1"
                     /translation="MRGLAFVLIAAAVGCKKPSDSGSGSAKPPPAPPVKIASVVASEA
                     PTPDVIVVTGLVAADQRSDVTADTQGKVINVLCDRGQRVKIGQPCVQLDVRSAALGAR
                     EAAANLAAARSSKQLADQECERTKSLLEKGAITKSEYDRQMAQCTSAQEQVAAVQART
                     EMMSKSVSDGMVRAPFDGIVSEKMVSPGEWVAPGKPLFTLVDDDPLKIEISVSEIAIR
                     ALKKDQRVEVVPTATPDKKYGATVTRLGAEIGRTRSLIVEATIDKGAELVPGMFAEAH
                     IQVGQTNRIVLPADAVVKRGKTWHAFVVKNGELEERLVQLGTAPAANQVSVVQGIVKG
                     EKVATKVDDKIVDGLRVVE"
     gene            complement(6218..7609)
                     /locus_tag="VMZ53_05805"
     CDS             complement(6218..7609)
                     /locus_tag="VMZ53_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUS27999.1"
                     /translation="MNVRLSLALVLAATTPALAQQAPRPAPVTAQAADDSFDKDLDAL
                     FTASGLTADQAASRAAKASPSVRRKAAEVEASLAQVQAAELARVPHLEAKAGYTRLSP
                     LDPLSFGPGGPAFEFPVNVYATNATIGIPLSEYVLRFPKLIDAAKLGVEAAKTGERAS
                     EVNASQEARLSYYELVRARLQVFVAERQLAQVQTVLKQVRALAEAQRVSKADLMRVES
                     NEAQAEQVLNQLRNVAALREEQLQLLMGADQPITLGEDPRTDVNAPGAGELDDLAKKA
                     TSQRLEFRAIDLGIQAREKQRESEKASTYPHLSAFASADYANPNQRIFPQTAEWNFTW
                     SAGVQLTWTLNDALQARATDRRITAETNELRADRENLARGTRIELLAAQQAVQLAQMS
                     LQTSQKGLAAAEEGYRVRRELLNAERATAVELVDAETDLTRARIAALNARVDLHVAMA
                     QLAHALGEDVAKK"
     gene            7798..9192
                     /locus_tag="VMZ53_05810"
     CDS             7798..9192
                     /locus_tag="VMZ53_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS28000.1"
                     /translation="MATATRTVTTAGTSLAPDASVPEAPRPAPGDVWRGRRNLYFIEE
                     TRTISRWAMMVGTLIHAAVIAILLHAHYPMWRIVSVGVLYVVFAIAQRFIIQRGQKIE
                     CVEASFVGANVTAQLFVTVLASLTGGLHSPLLPGLALPSLVSLLFFGPIAASRWIALC
                     NGLLVVAMLALPSSYVGPALPDFNYMLAVLLILGWSIFMLHMMAGKLANAAARAGETY
                     ECLREERVSEAEAQLRRLQSVGAKVAHELKNPLASIKGLCQLVARAPESERTQERLAV
                     VASEISRMETILNEYLSFSRPLEDLRPESLDLGAVARDVLDVLAGRADQAGVTLTIDG
                     HAAPVQGDPRRLKEALINLVANAIEATPHGGTVQVRVRSTSAGITLEVKDTGRGIAPE
                     DLERLGTSFFTTRPNGTGLGVVLAQGVIAQHGGALTYASAPGKGTTATIALPGKASAS
                     PEPAPRAAMVEARA"
     gene            9189..10574
                     /locus_tag="VMZ53_05815"
     CDS             9189..10574
                     /locus_tag="VMZ53_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS28001.1"
                     /translation="MTTVLLVDDEPGVLFTLSEVLTERGHRVLTAKSGAEALTKLDDA
                     ETVVTDLSMPGMDGLELMSQIVARDPALPILLLTAHGSERVAVAAMKNGAYDYLAKPF
                     DIDEIAVVIERALEARRLRVDNRRLAAEQTLGRRIIGASKPMRRLLEATSRVASKDVT
                     VLVRGETGTGKEFVAELLHAQSTRADKPLIRFNCAALPADLADAELFGHARGAFTGAN
                     ASRPGFFAQADGGTLILDEVGELPPQVQAKLLRALQEGEIQPVGSGRIDKVNVRVVAS
                     TNRDLAADVKAGTFREDLYYRLAVVELVVPPLRDRKDDIPALAEEFARRYGERFGLST
                     ITLEPSLIDALVRTDWPGNVRQLENTIARLAALSAGGVIGLADFRAADTSHSPSAPPS
                     SSISDTDEPAPDARNGPSLKEQVEAFERGLVARALDATGGNQSEAARRLGVSRVTLID
                     KMKKYGLGPKR"
     gene            complement(10571..10945)
                     /locus_tag="VMZ53_05820"
     CDS             complement(10571..10945)
                     /locus_tag="VMZ53_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28002.1"
                     /translation="MRYSLLIAMVLAACGDDGGSSTPHPDAAQMVDARMIDAAIDAPP
                     DAPPADNAAITMTCTDLCDAIGVCFMEPPDASCVTDCSADLADCTAQQVTDLDACKTE
                     ACGDETASPIFDCITAVSCVDG"
     gene            11288..12733
                     /locus_tag="VMZ53_05825"
     CDS             11288..12733
                     /locus_tag="VMZ53_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28003.1"
                     /translation="MSENATVTLTRTGYAGAVTLNVTGLPAGVTADPLTIGAGADSGT
                     LVLRSTAAAVLGSSAMATVTGDGSGAMKSAMVQVYVQGPHGSLDTTFGTGGKAIVPLG
                     TATNGDSVHGVVVMQNGDIVAVGGGSNATGAFGGAIALTPAGQVDTSMFNQGKHVGVY
                     TGGSNMADHFFAATLQPDGKILAAGFAGIGGQSHFYLLARFTTLGVLDTSFGLNSSGF
                     NVTDFDPAAGDDAKAYNLAVKPDGSIVAGGFYATSAATFGTTARYTSAGTLVGRLPAV
                     PPTFQAAVGARADNSVIAVGVQGTGLGIARYTAADGIDTTFGNGTGATTAVLRNAGSD
                     IPGSIIVLPDNKFYVGAYTKNGTNYEAALARFTDAGTLDTTFAGGTGFVTTPTNSPTN
                     WDKIAPTALLPDGSVVLAYTLAGTTNDFAWVHVKADGTIDGASNVVSTDFASGSDFVG
                     AVTVQPDGRIVLAGTAAANGRNNFAVARYWP"
     gene            complement(12734..13330)
                     /locus_tag="VMZ53_05830"
     CDS             complement(12734..13330)
                     /locus_tag="VMZ53_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28004.1"
                     /translation="MRATLRPFVVVPASPDFARVFDRDIARIRSTERARELGVIVLMP
                     RDVQPSTLMFAAYAWPQLLRGTGVTRIAVVLGIAAYAVAQPLLGGCVRRMADGGIHVE
                     FMHEAHVESGALLAWSERRKPRRRVTTDQLVRVCERYADRGDLRSAMLALKLAEDSAE
                     LEDEADEPAEPPKPAQRTVRMRGSSEDIGGRITADLVA"
     gene            complement(13369..13959)
                     /locus_tag="VMZ53_05835"
     CDS             complement(13369..13959)
                     /locus_tag="VMZ53_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28005.1"
                     /translation="MFKHPALRERSIDHDHARDRAHDASRKAPHPDHHVANAIGKATL
                     AERCTPRHLAPPDTRTHAIETARREIGAIRAVLMPAFIKALRTKNRIELSTATTQLRY
                     MLMRANQQVFSLEKAATYTDPLIVFLRKEVDTLAARATRLGAYKGVEHLVPRGYEDPI
                     KPNLPAIKRKPRPTDHKHEPAPPSAHVSRWFPKKAA"
     gene            14078..15055
                     /locus_tag="VMZ53_05840"
     CDS             14078..15055
                     /locus_tag="VMZ53_05840"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HUS28006.1"
                     /translation="MEGIVHICNGDSTADGLSLADLPGEIRVWADALDRGPVRPLPDD
                     EHYRARGEFWASRGEGAASDSAKKLADWDKGFDEAAGAEELILWYEHDLFDQLALARL
                     LARLHKRGLPATLTIVSIDRHPEIPNFLGFGQLEPTQLAELWPRRTPLSRDAIDEAVT
                     AWIAFTAGDPRALPFLTKRVKAMPFLGGALERLLEEYPDPTSGMSRTERQVLAAVARG
                     EGSAAQLMQQSQAGDPRYPITDAYLLDVLRELQRCGFVEGVPASGAASPDVFKGMTAS
                     VTATGRQALAGAIDRVHEQGIDEWRGGVHLSGRGPVWRWSSSERKLIEK"
     gene            complement(15067..15435)
                     /locus_tag="VMZ53_05845"
     CDS             complement(15067..15435)
                     /locus_tag="VMZ53_05845"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HUS28007.1"
                     /translation="MQRPESFEEFWPYYVSQHRNKTCRQLHFVGTSLAMGCLAVSPFY
                     PPAALAAPVVGYGFAWVGHFAFEKNKPASWGGVKAFAWSLRGDLRMWRKMAMGQMDAE
                     LARVEQSGEFDDVPAEVAAV"
     gene            complement(15446..16384)
                     /locus_tag="VMZ53_05850"
     CDS             complement(15446..16384)
                     /locus_tag="VMZ53_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28008.1"
                     /translation="MALAACKGAAHAPQGSGTSASGSGSAVGSASAVGSAGSGANVAS
                     IDAATAAATAAACVPGGKLVASVDDKRLRICSDAKKTCVDIDPTTGALTSTAYATVAA
                     PSLAAARVEARDKSLFACFNDKCREIGKNARAIIAKDPDVHTDGLFATTDLGALVIPK
                     VSPQLWDIGADKKLELRPPSGERDIGAMVPVGNHVFVSWLPAAEAMYRRSGVLAAPLF
                     SGGSQLAMLDNARSVALTPDHRVFIFGEGAPSKIELEPSVGNYDDWVQEPGALAVLAD
                     QTIAVVIERKAEAQLVLIDPQRATVFRRWPLPRCPG"
     gene            complement(16407..17840)
                     /locus_tag="VMZ53_05855"
     CDS             complement(16407..17840)
                     /locus_tag="VMZ53_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28009.1"
                     /translation="MSVARANGVVLESYTGERPKDAARLLGPVLEELARKKYDAGDTV
                     ARAYESQISRSSMTAGGMPADFAAQVDVGFKAWVGGRFDEAIKVLVPLVETAHGNSGE
                     FARNPALREPLLKGLIALALAYQRIGDLGAMQATFREIIRAYPDAQIARAKYGPDAAT
                     AFEGVAREVQAGGTGKLTVKLADDTGVVFIDEAYRAAGSTTVELVPGEYRVVAMLNKQ
                     PSRSHLVNVRANANVTVEIDVKLDQAVRTVGYAGLQFASESDREEHEAQYAAAFATAV
                     GANAVAVVGIDDVKGRPAVVGSLVSLQTGREIRRASIPVDPDPSTDRLRALARFLGGE
                     EPAPGLEVQFARAVEKGEGAGAGGGGGGGGGNHADTGAGGDGGGARWGGWRFVTGGLA
                     VVGIGVGATLVALDGKCSKEPPAGMQCNDLYATKTPGFVSLGAGAVFAGLSIYLFATH
                     GSSDHKSGVSFVAPTRGGAVAGYSLVW"
     gene            complement(17894..18445)
                     /locus_tag="VMZ53_05860"
     CDS             complement(17894..18445)
                     /locus_tag="VMZ53_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28010.1"
                     /translation="MGRPAALRFGLAILATGAACGGSKHDSAIGNSSRAGGGPDLTAA
                     YLCSIEDGGYHYPQMPCVVKNVGGKYVLAKLAGSQRFRGEVRAAANGLSFDGEYYCPW
                     GDCTQPMHGEFVRRAGGELVGKFDSVVVTMVPAPANVAWGGTSYGGDAYGGFGYGGWG
                     YGGSEYGMQLAAPAPSPRSNRRH"
BASE COUNT         3188 a         6286 c         5919 g         3168 t
ORIGIN      
        1 tgatcgaggt cgacggcaag caggtgcagc tgaccaccac gcagttcgag ctgatggcgc
       61 tgatgatccg ccgcatggca gccgaagctc atcagcccga gctcgtgcgt ggcttcgttc
      121 gcagctccga gctgattgca gacttgtcgt gggacacgcg cgaaccgagc gagaaccacg
      181 tgaagcagct ggtccgtcgc gttagacgcg cgttagtgaa atccgagatc ggggacctga
      241 tcgaatcgcg tcatcgcttc gggtatcggc tgcgggcaat tccgaagttc ggctgattgt
      301 gtttaacgtc gcaccatgcg acgtgccctt ggcctttcca tcgttctcgc cttccccgct
      361 ctcgctcacg ccggcgatcc gaagttcgaa tacggcaagg ccgaagaagt aaaggacgtc
      421 aaagacgtcg agtgggtggc aaccgccgaa gcaggtgtcg tcttcacgac gggcaactcc
      481 gagacgacga cggcaacggg cggcgtcaag gcgtcgcgca agaccggcaa gaacaagctc
      541 gcactcgaag cgagcgccgc gtatgcgaag tctggagcgc gcgtgctgct cgatcaaaac
      601 ggcaacggga tgatcgacaa cgcctccgag atccaggacg tccagacgat cacggcagag
      661 acgctcgcga gcaggctccg ctacgatcgc tttctcacgg agctcaactc gctgttcgtc
      721 gccgcgctcg caagccgcga cacgccggcc ggtaaagagc tcgtcctcgg tgcacaggcc
      781 ggttacagcc gctcgctgcg caagacggcg acgtcgctga cactcggcga gttcggctac
      841 gactactcgc acgaagatct cgtcaccgtc gatgttccgt cggtgtcgat tcactccgcg
      901 cgcgcattca tcggccacca cgcgacgatg acggagggcg cggacctcga tgcatcgttg
      961 gaggtgctga cgaacctcaa cagcgagaac atcacgaccg aggacgacaa cggcaatccg
     1021 tacgacggtg gcccgttcca ggatacgcgc gtgaacgcga agatctcgat ctcggcaaag
     1081 atcggccaga acctcgcgtt tcaatcggcc atcgaggtga agtacgaccg ccgcccgggt
     1141 ccgctgccgg tgaagaacct cgcaatgggc ttcacgccgg ctgccgacac gctggatacc
     1201 atcatgaagg cgtccttcat ctacacgttc gtcgccccga cgaagaagta atccgcttag
     1261 ccgcgcttgc tgcggtgcac gccgtttgtg cgcgtgcctt gctcttcgac cgtcgcggca
     1321 agctcctcga ggagcgcgcg cagcgacttc atcttcttgc tgccgagctg cgtagcccag
     1381 cgggcttcgg tggctgcggc aacgcgccgc acttcggcgg cccacacccg gcccttggcg
     1441 ccgagccgga cgcgcttgat cacgccgtgg tcaggatcgg ggaaccgctc gaccatccgc
     1501 atggtctcga ggtcgtcgat caggtcaccc acggtctgct tgggcagcga gactgccgtt
     1561 acaaggtccg caagtcggac acccgtattg atgtcagggc caatggcact gagcagctgt
     1621 acgtgggccg gacgaagcgc gggaaagcca agtcgctcca agcgcgtcgc aacctgcgcc
     1681 tggaggtcgt gctgcggcac atggagcagc gagcgaaggt cccggcttga acgagtttgt
     1741 cggttcgtct tcgtcatgat cacgagcacg atcgtgaggg agtcgcgtaa taccagagcc
     1801 cgatcgggat cggaaccctg atcgttcgta aatctcagct ccggagcatg atgcgacttc
     1861 cgcccgttcg cgcttgatca atgacgattc acttgctgcc ggtccgcttg tgacactgcg
     1921 tgaatgtcga cgattccggc ttctgctttg ccgcggtcgg tatcctcggg gaagactttg
     1981 cgccaaaccc agcggaagaa ccgcgtcaga tcgtcgaacc acgtgtagat gacgggcact
     2041 gcgacgagcg tcaggagcag ggacaatgtc tgcccaccga cgacgacact cgccatcgcc
     2101 ttcgagaatc ccgagccgat accctggctc gtgatgagcg ggaccatgcc tgcgacgaac
     2161 gcaaacgtgg tcatcagaat cgggcgaagt cgatcgcggc tggccgccag gacagcttcg
     2221 gtccgaggca gaccgagccg gcgcagaccg ttggcgtggt cgacctggag gatgccgttc
     2281 ttcttcacca tcgcgaacaa cacgatgatg ccgagcatcg agaagatgtt gagcgacccg
     2341 cccgtgatga gcagcgacgc gaccgcaaac ggtacggtca acggcagcgc catcatgatg
     2401 atgatcggat agacccacga ttcgaactgc gccgcgagca cgaggtacat gaagacgaag
     2461 gcgagcagga tggcgaacaa gaatgccttg cccatcttcg ccatctcctt gctgcggccg
     2521 aacggctccg cggtgtaccc gggcggcagg tcgagctctt tgatgacctt ttgcagctca
     2581 gcgacgatcg ccgactcgtt gaagccaggc gacacgttca tcgtgatggt gacggcgcgt
     2641 tcgcggctct ggcgcgtgat cttcgagatc gctttgccgg cactcggctg gatcacgtcg
     2701 accagcggca ccgtgccgag cgtgcgcgac gggacgccga tgagcgcaag tgcgttcggg
     2761 tcattgcggt agcgctcggc cgcacggagg aacaccgggt actggtcgcc gcgatcctcg
     2821 aacgtcgagg cctcggtgcc accgacgagg acggacagcg tcatcgtgac gtcctgcgga
     2881 tcgacgccga gcatggcggc gcgattgagg tccggcttga gcgtggtctc atcgatcggg
     2941 tcgacgaggc tcgagtcgag atcgacgacg cccggcacct tcttcatctt ctcgagcacg
     3001 gttttgccgt agtgctcgag cttgtcgaga tcggggcccg agatgacgta cgagacgatg
     3061 gcgttctgac caccgatcga gaagtcgttg acctgttgca cggcgacgcg cgtgccctcg
     3121 ggaacatcgg gcaggacctt cctgcggacc tcttccatga cctcggcctg gttgcgcgag
     3181 cgcagctcgg ggttggtcat gtgcgcgtag acgttcgcga cgttcgactg gcgttggtca
     3241 gagccggcga ccgtgacgag tgtggcgtcg acttcgggaa tctggcgcgt cttgcgagct
     3301 aggcgctcgg cgacgagcgt ggtggcctcg agcgagctgc cctcgggcat ctgcacgtag
     3361 atctcgaact gcgcttcgtc gttcggcggg aggaagtcgc cgccgacctt cttgcacatc
     3421 ggcaccgtgc cgacgcacga gcccacgatt gcgatcgcca cgacccaccg gtggcgcaag
     3481 cagaacgcga ggatgccgga atacacgcgc gcgatcggct gatagatggc gtcgctaagc
     3541 ttctcgagtg ccgagcggcg acgctcctga ccttcgggtg gcggcggcgg caggatgcgt
     3601 gcggccatca tcggtgtcag cgagaacgcg acgatcatcg agaccgcgat cgcgaacgcc
     3661 atcgtgatgc cgaagctgta gaggaagcga ccgacgatgc cgctcatgaa cgcgacgggg
     3721 aggaacaccg ccatcagcga cagcgtcgtc gcgagcacgg ccaggccgat ttccttcgtc
     3781 gcgtgaatcg ccgcggggaa cggcttcatc cgcttctcgt cgatgaaccg atagatgttt
     3841 tcgagcacga cgatcgcgtc gtcgatcacg atgcccacgg cgagcgcgag cgcgagcagc
     3901 gtcatcatgt tgagcgtcag gcccgcgatc atcatcaggc cgaacgtcga gatgatcgaa
     3961 acggggatcg cgatcgcggc gatgatcgtc gagcgaaggc tgccgaggaa cagcagcacg
     4021 actagcgcgg cgagcagcgc gcccacgaag aggtgctcga gcacctgatc cgcagaggtg
     4081 cgaatctgga tcgagttgtc gcgcacgatg tccacggagt agccgggcgg gaggttcagc
     4141 tcctcgaccg ccgccttcac cgcgtcgacg acagcaacgg tgttcgtgcc cgtttgcttg
     4201 cggaccgaca gcgcgatggc cggcgtgccg ttacggacgg cagcggtctc ggcatcctct
     4261 tcggagtcga tgagcgtggc gacgtcgttg acacgaaccg cgtgctcgcc ctgctggcgc
     4321 acgacgatct caccgatctc ctttgcttcg atcgcgcggc cctcgatgcg aagcgtcatg
     4381 ttcttcggac cggtctggaa ctgaccaccc ggaacgttga cgtttgcgat cgcgatggcg
     4441 cgctggacct cgagtgcgga gacgcccgtc gcagcgaggc ggatcggatc gagctcgacg
     4501 ttgatctgcc gttcttgacc gccgaggatg acgatctgac cgacgccttt gaggcgttcg
     4561 atgcgttgct tgaccttctt gtcggcgaac cgcgtgagct cgcgcgtcga catgccggct
     4621 ggccccttga cggacagcac gaccaccggt gcggcatcag ggtccgcttt ccggacctcg
     4681 ggccgaacac ctggaggaag atcgcgaagc accgtattga gatgctcctg aatgtcctgc
     4741 gcggctttgt ccgggctcac ctcgagcgcg aactgcgcga tgacgaggga cacgccttcg
     4801 gtcgacaccg aggtcagcgt ctcgagaccg ttcaccgtgt tgacggcttc ttcgatcttt
     4861 tgggtgacgt ccgtctcgac agcgcctggc gaagcacctt ggtacggcgt cacgacggtg
     4921 acgagcggga aatcgacctt cgggaactta tcaacgccga ggcgcgaata gccgaccgcg
     4981 ccgatgacga cgagcacgag gatcaggacg cacgcgaaga ccgggcgtct gacgcagacg
     5041 ttggctaacc actgcacggt ttactccacc acgcggaggc catcgacaat cttgtcgtcg
     5101 accttggttg cgaccttctc gcccttcacg atgccctgca cgaccgagac ttgattcgcg
     5161 gccggcgcgg tgccgagctg cacgaggcgc tcttcgagct cgccgttctt gacgacgaat
     5221 gcgtgccacg tcttgccgcg cttcacgaca gcatcagcgg ggagcacgat gcgattcgtc
     5281 tggccgacct gaatgtgtgc ctcggcaaac atgccgggaa ccagctcggc gcccttgtcg
     5341 atcgtcgctt cgacgatgag cgagcgcgtg cggccgattt ctgcgccgag ccgcgtgacc
     5401 gtggcgccgt acttcttgtc gggcgttgcg gtcgggacga cctcgacgcg ctgatctttc
     5461 ttgagcgcgc ggatcgcgat ttcggagacg gagatctcga ttttcagggg atcgtcgtcg
     5521 acgagcgtga acagcggttt gccgggcgcg acccactcac cgggtgacac catcttctcg
     5581 gacacgatgc cgtcgaacgg cgcgcggacc atgccgtcgg agaccgactt cgacatcatc
     5641 tcggtgcgcg cctgcaccgc ggctacttgc tcctgcgccg atgtgcactg cgccatctgc
     5701 cggtcgtact cggacttcgt gatcgcgccc ttctcgagca gcgacttcgt acgttcgcat
     5761 tcctgatcag cgagctgctt cgacgagcga gctgccgcga ggttcgctgc agcctctcgc
     5821 gcgccgagcg ccgcgctgcg gacatcgagc tgaacgcacg gttggccgat cttcacgcgc
     5881 tggccgcggt cgcacaggac gttgatgacc ttgccctgcg tatccgccgt gacgtcggag
     5941 cgttggtcgg cagcgacgag gccggtcacg acgatgacgt ctggcgtcgg ggcctcgctc
     6001 gcgaccacgc tcgcgatttt caccggcggt gccggcggcg gcttggccga cccagacccc
     6061 gaatccgatg gcttcttgca gccaactgcc gccgcgataa gaacaaacgc tagaccacgc
     6121 atcgtggcgg ctacgatgca cgccctttgc cattcggtac agcggactta ccaagggtta
     6181 gccgcgccgg gctgtaagcc ttcccgacac tgccgggtta cttctttgcg acgtcctcgc
     6241 caagggcgtg agcgagctgc gccatcgcga cgtggagatc gacccgcgcg ttgagggcgg
     6301 cgatgcgggc gcgggtcaga tccgtctcgg cgtcgacgag ctcgacagcg gttgcgcgct
     6361 cggcgttgag cagctcgcga cgaacgcggt agccctcctc cgctgcggcg aggcccttct
     6421 gcgaggtctg cagcgacatc tgcgcgagct ggaccgcctg ctgcgccgcg aggagctcga
     6481 tgcgcgtgcc ccgcgcaagg ttctcgcgat cagctcgcag ctcgttcgtc tcggcagtga
     6541 tgcggcgatc ggtggcacgc gcctgcagtg catcattgag cgtccacgtc agctgcacac
     6601 cggcggacca ggtgaagttc cactctgcgg tctgcgggaa gatgcgctgg ttcgggttcg
     6661 cgtagtccgc gctcgcgaat gcggagagat gaggatacgt gctcgccttc tcgctctcgc
     6721 gctgcttctc cctggcctgg atgccgaggt cgatcgcgcg gaactcgagc cgctgcgacg
     6781 tcgccttctt cgcgagatca tcgagctcgc ctgcgccggg cgcattgacg tcggtccgcg
     6841 gatcctcgcc gagcgtgatc ggctggtccg cgcccatgag gagctggagc tgttcctcgc
     6901 gcagcgctgc gacgttgcgc agctgattca acacctgctc cgcctgcgct tcgttcgact
     6961 cgacgcgcat caggtccgcc ttcgacacgc gctgcgcctc ggcgagcgca cgcacctgct
     7021 tcagcacggt ctgcacttgt gcgagctggc gctcggcgac gaagacttgg agacgcgcgc
     7081 ggacgagctc gtagtacgag agcctcgcct cttgcgacgc attcacttcg ctcgcgcgct
     7141 cgcccgtctt cgctgcctcg acgccaagct tcgcggcatc gatcagcttc gggaaccgca
     7201 gcacgtactc cgagagagga atgccaatcg tcgcgttcgt cgcgtagacg ttcaccggga
     7261 actcgaacgc cgggcccccc gggccgaacg agagcggatc gagaggagac agtcgcgtgt
     7321 agccggcctt cgcttcgaga tgcggaacgc gcgcgagctc ggcggcctgc acctgcgcga
     7381 gcgatgcctc gacctcagcc gccttccgcc gcaccgaagg agacgctttc gccgcgcgag
     7441 atgcagcttg atccgccgtg agcccgctcg ccgtgaacag cgcgtcgagg tccttgtcga
     7501 agctgtcgtc agctgcctgc gcggttaccg gagcgggacg gggcgcctgt tgcgccagtg
     7561 caggagtcgt cgcggccaaa accaaagcca agctcaggcg aacgttcatg cggcccacca
     7621 aagcaccagc cgtgccacgg gtccagccgc ctagtgctcg gagttccggt gcagcgggct
     7681 gggatctgtc gggcagcctt acagcctgca gtcacgtttc gtgacatcag ccagtcacgt
     7741 ttcgatcgcc gaggattgct tccttcgcaa cacattgttc gggctagagt tggcctcatg
     7801 gcgaccgcga cgcgaactgt caccacggct ggcacgagcc tggcaccaga cgcgagcgtg
     7861 cccgaagccc cgagacccgc gccaggtgat gtttggcgcg gccgccgcaa cctctacttc
     7921 atcgaggaaa cgcggacgat ttcacgttgg gcgatgatgg tcggcacgct gatccacgcc
     7981 gcggtgatcg cgatcttgct gcacgcgcac tatccgatgt ggcgcatcgt cagcgtgggc
     8041 gtcctctacg tcgtcttcgc gatcgcgcag cgcttcatca tccagcgcgg ccaaaagatc
     8101 gagtgcgtgg aggcgtcgtt tgtcggcgcg aacgtcaccg cgcagctgtt cgtcacggtg
     8161 ctcgcgtcgc taacgggcgg cctacactca ccgctactgc ccggcctcgc gctcccttcg
     8221 cttgtctctc tactcttctt cggtccgatc gcagcgtcgc gctggatcgc gctctgtaac
     8281 ggcctgctcg tcgtcgcgat gctcgcgctg ccctcgtcat acgtgggccc tgcgctgccc
     8341 gacttcaatt acatgctcgc cgtcctgctg atcctcggct ggtcgatctt catgctgcac
     8401 atgatggccg gcaagctcgc gaacgccgcc gctcgcgcag gcgagacgta cgagtgcctc
     8461 cgcgaagagc gagtctccga ggctgaagct cagctgcgcc gcttgcagtc cgtcggagcg
     8521 aaggtcgcgc acgagctgaa gaacccgctc gcatcgatca aaggtctctg ccagctcgtt
     8581 gcacgcgccc ccgaaagcga acgtacgcaa gaacgcctcg cggtcgtcgc ctccgagatc
     8641 agccgcatgg agacgatcct caacgagtac ctctcgttct cacgtccgct cgaagatctg
     8701 cgacccgagt cgctcgatct cggtgctgtc gcgcgcgacg tcctcgacgt cctcgcaggc
     8761 cgcgccgatc aagcaggcgt cacgctcacg atcgacggcc acgcagcgcc ggtgcaaggc
     8821 gatcctcgcc gcctcaaaga ggcgctcatc aacctcgtcg caaacgcgat cgaagccacc
     8881 ccccacggcg gcacggtcca agttcgcgtg cgctccacgt ccgcgggcat cacgctggaa
     8941 gtgaaagaca ccggccgcgg catcgcgccc gaagatctcg agcgcctcgg cacctcgttc
     9001 ttcacgacgc gcccgaacgg tacaggcctc ggcgtcgtgc tcgcgcaagg cgtcatcgct
     9061 caacacggtg gcgctctcac ctacgcgagc gctccaggca aaggcaccac cgccacgatc
     9121 gcgctccccg gcaaggcgtc ggcatctccg gaacccgcgc cacgcgccgc gatggtggaa
     9181 gcacgcgcat gacgacggtc ctcctcgtcg acgacgagcc cggcgttctg ttcacgctca
     9241 gcgaagtcct caccgaacgc ggccaccgcg tgctcactgc aaagtcgggc gccgaagcac
     9301 tcacgaagct cgacgacgcc gaaaccgtcg tcaccgatct ctcgatgccc ggcatggatg
     9361 gcctcgagct gatgtcgcag atcgtcgcgc gagacccggc gctgcccatc ctgctgctca
     9421 ccgcgcacgg ctccgaacgc gtggcagtcg ccgcgatgaa aaacggcgcg tacgactacc
     9481 tcgccaagcc attcgacatc gacgaaatcg ccgtcgtgat cgagcgcgcg ctcgaagctc
     9541 gccgccttcg cgtcgacaac cggcgcctcg ccgccgaaca aacactcggc cgccgcatca
     9601 tcggtgcttc caagccgatg cgccgcctgc tcgaagcaac gtcgcgcgtc gcctccaaag
     9661 acgtcaccgt cctcgtgcgc ggcgaaaccg gaacgggcaa agagttcgtc gccgagctcc
     9721 tccacgccca atcgacgcgc gccgacaagc cgctcatccg cttcaactgc gccgcccttc
     9781 ccgccgacct cgccgatgcg gagctcttcg gccacgcgcg cggtgcattc accggcgcca
     9841 acgcctctcg ccccggcttc ttcgcgcaag ccgacggcgg caccctcatc ctcgacgaag
     9901 tcggcgagct ccctccgcaa gtccaagcca agctcctccg cgcgctccaa gaaggcgaaa
     9961 tccagccagt cggctcgggc cgcatcgaca aggtcaacgt gcgcgtcgtc gcctccacaa
    10021 atcgcgacct cgccgccgac gtcaaagccg gcaccttccg cgaagacctc tattaccgcc
    10081 tcgccgtcgt cgagctcgtc gtccctcccc tccgcgatcg caaagacgac atccccgccc
    10141 tcgccgaaga attcgcgcgc cgctacggcg aacgcttcgg cctgagcacg atcacgctcg
    10201 aaccctctct catcgacgcg ctcgtcagaa ccgactggcc cggcaacgtg cgccaactcg
    10261 aaaacacgat cgcgcgcctc gccgctctct ccgcaggcgg cgtcatcggc ctcgccgact
    10321 tccgcgcagc cgacacctcg cattccccat ccgccccacc ctcctcctcg atcagcgaca
    10381 ccgacgagcc cgcgcccgac gcgcgcaacg gcccctcgct caaagagcaa gtcgaggcct
    10441 tcgaacgcgg cctcgtcgca cgcgccctcg acgcgacagg cggcaaccaa tcagaagccg
    10501 cacgccgcct cggcgtctca cgcgtcacgc tgatcgacaa gatgaaaaaa tacggcctcg
    10561 gcccgaaacg ctaaccgtcg acgcaactca ctgcggtgat gcagtcgaag atcgggctcg
    10621 ccgtctcgtc gccgcatgcc tcggtcttac acgcgtcgag atccgtcacc tgctgcgcgg
    10681 tgcaatccgc cagatccgcc gagcagtccg tcacgcagct cgcatccggc ggctccatga
    10741 agcagacgcc gatcgcgtcg cacagatccg tgcacgtcat cgtgatggcc gcattgtcgg
    10801 ccggcggtgc atccggcggc gcatcgatcg cggcatcgat catccgcgcg tcgaccatct
    10861 gcgcagcatc aggatgcggc gtcgaagatc cgccgtcgtc gccacacgcc gccaacacca
    10921 tcgcgatcaa gagcgagtaa cgcatggcgc atcagatagc ccacggcgcc ggagcgttgt
    10981 ctggcaatct gcatgcagct gcgctttgcg cagattgcgc cacgcgcgtc gctccacgtg
    11041 tcaatgtgag ccggctctgc caaggccgcg gttgtgattg gccgcaccta tccgggggct
    11101 cgacggccgc gacgcaggac gcggactttg ggatactgct ccgatgaagc agggtgtcct
    11161 tggtttggct atttggctgg tagcagcatg cggcagtgtc tccgaaaaca ggcccgacgc
    11221 cgccatcgac gctggaggtg acttcagtct cgcagtgact ccggcgccac tgcgcatcgt
    11281 gcaaagcatg tccgagaacg cgacggtgac gctaacccga accggctacg ccggggccgt
    11341 caccttgaac gtcaccggcc ttcctgcagg cgtgaccgcg gatccactca cgatcggcgc
    11401 tggcgctgat tccggtacgc tggttctgcg gagtacggcc gccgcagtgc tgggcagctc
    11461 tgcgatggct acggtcacag gcgacggctc cggcgcgatg aagagcgcga tggttcaggt
    11521 ctacgtgcaa gggccgcacg gctcgctcga cacgacgttc ggcaccggtg gcaaggcgat
    11581 cgtgccgctc ggcacggcca ctaacggtga cagcgtccat ggcgtcgtcg tgatgcaaaa
    11641 cggcgacatt gtcgcggttg gcggtggatc gaatgcgacc ggtgcattcg gtggtgcgat
    11701 cgcacttacg cctgctgggc aggtcgacac cagcatgttc aaccaaggca agcacgtggg
    11761 cgtgtacacc ggcggtagca acatggccga tcacttcttc gccgcgaccc tgcaacccga
    11821 cggaaaaatc ctggcagcag gcttcgccgg tatcggaggg cagtcgcact tctatctgct
    11881 cgcgcgcttc accacgctcg gcgtcctcga cacgagcttt ggtctgaaca gctcgggctt
    11941 caacgtgacg gatttcgatc ctgctgcggg cgacgacgcg aaggcctaca acctcgccgt
    12001 caagccggac ggctcgatcg tcgcgggcgg tttctacgcg accagcgccg cgaccttcgg
    12061 aacgaccgcg cgctacacca gcgccggcac gctcgtcggt cgcttacccg ccgtacctcc
    12121 cacgttccaa gcagcagtcg gtgctcgcgc cgacaacagt gtgatcgcag tcggcgttca
    12181 gggaacggga ctcggaatcg cgcgctacac cgcagctgat ggcatcgata cgacgttcgg
    12241 caacggcacc ggagctacga ctgctgtctt gcgcaacgcc ggcagtgaca tccccggcag
    12301 cattatcgtg cttccggaca acaagtttta cgtcggcgcg tacacgaaga acggcacgaa
    12361 ctacgaagcc gcgctcgcgc gcttcacgga cgcgggaaca ctcgacacga cgttcgctgg
    12421 tggaaccggt ttcgtgacga cgccaacgaa cagcccgacg aactgggaca agattgcgcc
    12481 gacggcgctc ctccccgacg gctccgtcgt cctcgcctat acgctcgcag gcacgacgaa
    12541 cgacttcgcg tgggtacatg tgaaggcgga cggtacgatc gacggagcgt cgaacgtcgt
    12601 ctctacggac ttcgcatccg gatctgactt cgtcggtgcg gtgaccgtgc aaccggacgg
    12661 tcgtatcgtc ctcgcgggga ccgctgctgc gaacggtcgc aacaacttcg ccgtcgctcg
    12721 ttactggccg tagtcaggcg acgaggtccg cggtgattcg tccgccgatg tcctcgctgc
    12781 ttccgcgcat gcgcacggtg cgctgcgcag gcttcggcgg ttccgccggt tcgtccgcct
    12841 cgtcctcgag ctccgcgctg tcttctgcga gcttgagcgc gagcattgcc gagcgcaggt
    12901 cgccgcgatc ggcgtagcgc tcgcagacgc gcacgagttg atccgtcgtg actcggcgac
    12961 gcggcttgcg tcgttcgctc cacgcgagca gcgcgcccga ttcgacgtgc gcctcgtgca
    13021 tgaactcgac gtgaatgccg ccgtctgcca tgcggcgtac gcaacctccg aggagcggtt
    13081 gcgcgacggc gtacgcggcg atgccgagca cgacggcgat gcgcgtgacg ccggtgccgc
    13141 ggagaagctg aggccacgcg tacgcggcga acatcagcgt gctcggttgg acgtcgcgcg
    13201 gcatcagcac gatgacgcct agctcgcgtg cgcgctccgt cgagcggatg cgagcgatgt
    13261 cgcgatcgaa gacgcgcgcg aagtcaggag aggccgggac gacgacgaag gggcgcagcg
    13321 ttgctctcat gtcgctgcta tcgaccgcac catttcgtgg ttgcgcgatc atgctgcctt
    13381 cttcgggaac cagcgcgaga cgtgggcact gggcggcgcg ggctcgtgtt tgtgatcggt
    13441 gggacgcggc ttgcgtttga tcgcggggag gtttggcttg atcggatctt cgtagccacg
    13501 cgggacgagg tgctcgacgc ctttgtaagc gccgaggcgc gttgcgcgtg cggcgagcgt
    13561 gtcgacttcc ttgcgcagga agacgatgag cggatcggtg tacgtcgcgg ctttctcgag
    13621 gctgaagact tgctggttgg cgcgcatcag catgtagcgg agctgcgtcg tcgctgtgga
    13681 gagctcgatg cgattcttgg tgcggagggc tttgatgaag gcgggcatga ggaccgcgcg
    13741 gatcgcgccg atttcgcggc gagcggtttc gatcgcgtgc gtgcgcgtgt ccggcggcgc
    13801 gaggtggcgc ggcgtgcagc gttcggcgag ggtcgctttg ccgatcgcgt tcgcgacgtg
    13861 gtggtccggg tgcggcgcct tgcgtgatgc gtcgtgcgcg cgatcgcggg cgtggtcgtg
    13921 gtcgatggag cgctcgcgga gggccggatg tttgaacatg gcgcttgtat cggcgcggcc
    13981 ggcgagcggt tgcgtcggtg ggagaagggc tggcggcgcg gccacttagg ggcgcgctcc
    14041 cggggtcagc ggcggcgggc tgtgctacgc ccggcgcatg gaaggcatcg tccacatctg
    14101 caatggcgac tcgacggcgg atggcctctc gctcgctgat ttgccgggcg agattcgcgt
    14161 ctgggcggac gccttggatc gcgggccggt gcggccgctg ccagacgacg agcactatcg
    14221 ggcgcgcggt gagttttggg cgtcgcgcgg tgagggagcg gccagcgaca gcgcgaagaa
    14281 gctcgccgat tgggacaagg gcttcgacga agcagcgggc gctgaagagc tcattctctg
    14341 gtacgagcac gatctctttg atcagctcgc actcgcgcga ctgctggcgc gactgcacaa
    14401 gcgcggcttg cccgcgacgc tcacgatcgt gtcgatcgat cggcatcccg agattccgaa
    14461 ctttctcggg ttcgggcagc tcgagccgac gcagctcgcg gagctgtggc cgcggcgtac
    14521 gccactctcg cgcgatgcga tcgatgaagc cgtaacggcg tggatcgcgt ttacggcggg
    14581 cgacccgcgc gcgctgccgt ttctaacgaa gcgcgtgaag gcgatgccgt tcttgggcgg
    14641 cgcgctcgag cgcttgctcg aggagtatcc cgatccgacg agcgggatgt cgcgcacaga
    14701 gcggcaggtg ctggcggcgg tggcgcgcgg cgaaggaagc gcggcgcagc tcatgcagca
    14761 gtcgcaagcg ggagatccgc gctatccgat cacggatgcg tacctgctcg acgtgctgcg
    14821 cgagctgcag cgctgcggct tcgtcgaggg agtgccggcg agcggcgcgg cgagccccga
    14881 cgtcttcaaa ggcatgaccg cgagcgtcac cgcgaccggc cgtcaggcac tcgccggcgc
    14941 gatcgatcgt gtgcacgagc aaggcatcga cgagtggcgc ggtggcgtcc acctcagcgg
    15001 gcgtggtccc gtctggcgat ggagctcgag cgagcgcaag ttgatcgaga agtagcgacg
    15061 cgccgcttag acggcagcga cttcggccgg cacgtcatcg aattcgccgc tctgctcgac
    15121 gcgcgcgagc tcggcgtcca tctggcccat cgccatcttg cgccacatgc ggaggtcacc
    15181 gcgcagcgac cacgcgaacg ccttcacgcc gccccacgat gcgggcttgt tcttctcgaa
    15241 cgcgaagtgg ccgacccacg cgaagccgta gccgacgacg ggcgcggcga gcgcggctgg
    15301 cggataaaac ggcgacaccg cgaggcagcc catcgcgagc gacgtgccga cgaagtgcag
    15361 ctgccggcag gtcttgttgc ggtgctgcga gacgtaatag ggccaaaatt cttcgaagct
    15421 ttcggggcgc tgcatgcgga cgagactagc ccggacaccg cggcaggggc cagcgccgaa
    15481 atacggtggc gcgctgcgga tctatgagca cgagctgcgc ttcggccttg cgctcgatga
    15541 ccaccgcgat ggtttggtcc gcgagcaccg cgagtgcgcc aggttcctgc acccagtcgt
    15601 cgtagttgcc gacgctcggc tcgagctcga ttttcgacgg cgcaccttcg ccgaagatga
    15661 acacgcgatg gtcaggtgtc agcgcgaccg accgcgcgtt atcgagcatc gcgagctgcg
    15721 agccgccgga gaacagcggc gcagcgagca caccgcttcg gcgatacatc gcttcggcgg
    15781 ctggcagcca gctaacgaat acgtgattgc cgacgggcac catcgcgccg atgtcacgct
    15841 cgccgctcgg cggtcgaagc tcgagcttct tgtcagcgcc gatgtcccac agctgaggag
    15901 agactttcgg aatgacgaga gcgccgaggt ccgtcgtggc gaagagacca tcggtgtgga
    15961 cgtccggatc cttcgcgatg atcgcgcgcg cgtttttgcc aatctcgcgg cacttgtcgt
    16021 tgaagcacgc gaacaagctc ttgtcgcgag cctcgactct cgcggcggcg agcgatggcg
    16081 ctgccactgt ggcgtacgcg gtcgacgtca gcgcgccggt cgtcggatcg atatcgacgc
    16141 acgtcttctt cgcgtcgctg cagatgcgca ggcgtttgtc gtcgacgctc gcgacgagct
    16201 tgccgcccgg cacgcacgca gcggcggtcg cagcggcggt cgctgcatcg atgctagcga
    16261 cgttcgcccc cgagccggcg ctaccgaccg cgctcgcgct acctaccgcg ctgccgctac
    16321 cactcgcact agtgccgctg ccttgtggtg catgcgctgc gcctttgcac gcggcgagcg
    16381 ccatcactgc agcgagcgcg agcgacctac cagacgagcg aatatccagc aacggctcca
    16441 cctcgcgtcg gcgcgacgaa cgacacgccg ctcttgtgat ccgagctgcc gtgcgtcgcg
    16501 aataaataga tcgagagtcc tgcgaacacg gcgcccgctc cgagcgacac gaagccaggc
    16561 gtcttcgtcg cataaagatc attgcactgc atcccggccg gcggctcctt cgagcacttg
    16621 ccgtcgagcg cgacgagcgt cgcacccacg ccgatgccga cgactgcgag cccaccagtc
    16681 acgaagcgcc aaccgcccca acgcgcgcca ccaccgtcgc cgcccgcgcc cgtgtcggcg
    16741 tgattgccgc cgccgccacc gccgccgccg ccggccccag cgccctcgcc tttctcgacc
    16801 gcgcgcgcga actgaacttc gagccccggc gcgggctctt caccgccgag aaaccgcgcc
    16861 agtgccctca accgatctgt cgacggatcc ggatctacag gaatgctcgc gcgccgaatc
    16921 tcgcgtccgg tctgcagtga cacgagcgaa ccgacgacag cgggcctgcc cttgacatca
    16981 tcaatgccga caacggccac ggcattcgca cctaccgccg tcgcaaacgc cgccgcgtac
    17041 tgcgcctcgt gctcttcgcg atcgctttcg ctcgcgaatt gcagccctgc gtagccgacg
    17101 gtgcgcacag cttggtcgag cttcacgtcg atctcgaccg tcacgttcgc attcgctctc
    17161 acgttcacga gatgactgcg cgatggctgc ttgttcagca tcgcgacaac gcgatactcg
    17221 ccgggcacca gctcgaccgt cgtcgatcca gccgcgcggt atgcctcgtc gataaacacg
    17281 accccggtgt catcggccag cttcacggtc agcttgcccg ttccacccgc ctggacctcg
    17341 cgcgcaacgc cttcaaaggc cgtcgccgca tccggcccat acttcgcgcg cgcaatctgc
    17401 gcgtcgggat acgcacgaat gatctcgcga aacgtcgcct gcatcgcgcc caggtcgccg
    17461 atgcgctgat acgcgagcgc caacgcaatc agccccttca acaacggttc gcgcagcgcc
    17521 ggattgcgcg cgaactcgcc gctattccca tgcgccgtct cgacgagcgg caccaacacc
    17581 ttgatcgcct catcaaagcg cccaccgacc cacgccttga acccgacatc cacctgcgca
    17641 gcaaaatccg cgggcatgcc gcccgccgtc atcgacgatc gcgagatctg cgactcgtac
    17701 gcacgcgcga ccgtatcgcc agcgtcgtac ttcttgcgcg ccagctcctc gagcaccggc
    17761 ccgagcaacc gcgccgcatc cttcggccgc tcgcccgtat acgactcgag caccacgccg
    17821 ttcgcacgcg cgaccgacac aaacgccacc aaaaccacga atacgcgaaa caccattgcc
    17881 cctctatcgg ccctcaatgc cgccggttgc tccgcggcga cggcgccggc gccgctaact
    17941 gcatcccata ctcgctgccg ccataacccc agccgccata tccaaagccg ccatacgcat
    18001 cgccgccgta gctggtgccg ccccacgcaa cattcgccgg cgcaggcacc atcgtcacca
    18061 cgacgctgtc gaacttccca acgagctcgc cgcccgcccg ccgcacaaac tcgccatgca
    18121 tcggctgcgt gcaatcgccc cacggacagt agtactcgcc gtcgaagctc agcccgttgg
    18181 ccgccgcgcg cacctcgccg cgaaaccgct gcgacccagc aagcttcgcg agcacgtact
    18241 tgccgccgac attcttcacg acgcacggca tctgcggata gtgatagccg ccgtcctcga
    18301 tggagcacaa atacgccgcc gtcaggtcag gcccgccgcc agcgcgcgat gaattgccga
    18361 tcgccgaatc gtgcttgctg ccgccgcacg ccgctccagt cgcgagaatc gcgagcccga
    18421 aacgaagtgc ggcaggccta cccatggggc gagcctgccg cttgcgcgac caagtttcaa
    18481 cgcgcttagg gcaccgtctc gagatactgg tagttggccg agatgccttg cttcatgagc
    18541 gcggggtcgg cgcccggatt g
//
LOCUS       DATLBZ010000111        27257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2343227, whole genome shotgun sequence.
ACCESSION   DATLBZ010000111 DATLBZ010000000
VERSION     DATLBZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 27257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27257
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343227"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VMZ53_05865"
     CDS             complement(<1..538)
                     /locus_tag="VMZ53_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28011.1"
                     /translation="MNVRSSNALLVLALLGGCSGKTKGVEDARKHPQDSAPVTDPTAP
                     PPSGKGDVQLRVEWKDVPLAARASSGRTPCNTPRAASVAPTTTWGIPEVFVMIDAPPA
                     PQAASSSGSNGTRATTGSAPTGSATAPAMTARAMTREARVVLDHCALTPRVALASDKL
                     IVASAAESPAKVVITKAGA"
     gene            complement(543..1919)
                     /gene="hemG"
                     /locus_tag="VMZ53_05870"
     CDS             complement(543..1919)
                     /gene="hemG"
                     /locus_tag="VMZ53_05870"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HUS28012.1"
                     /translation="MRVAVVGGGLGGLVAAHGLVSAGFDAHVLEAAPAPGGVIGTSIV
                     DGFVREHAASSFLGGPSRGALALCQELGIEVEAASPRAKTRWIYIDGKLRALPRNPID
                     FLRSDLLTWRGKLALLREPLAPRKGTEDESIHAFAARRFGAEAARAIVAPFVTGIFAA
                     DSHAISLEAGFPRLAQFDAEGGVVRSAAKQVGKSLLVKLSGKRTAKTPSGMWAPAGGL
                     GSLVEALTTKLGPRIRTHRRIRTIAPAPATAGVLVDNERWDAVVLATPAHEGASLVDD
                     AMPELASRLRVFERAPVALVYLGLPADSVPRASDGFGFLTAQGEDLRVLGVVFESTVW
                     SNRAPEGQALLRCIFGGGRDPDATKLTDAELIEHALRDVAKAFASTKKSRGAMTTLPA
                     PTHASVIRWSHGIAQYPVGHRDYVRAAVAAGRVHRIALAGADYRGAGVNDLCADRDVI
                     VSELRTWS"
     gene            complement(1916..2950)
                     /gene="hemE"
                     /locus_tag="VMZ53_05875"
     CDS             complement(1916..2950)
                     /gene="hemE"
                     /locus_tag="VMZ53_05875"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HUS28013.1"
                     /translation="MSEHLFLRACRRQPVPRTPIWMMRQAGRYLPAYRAVRAKASFLD
                     LCRTPELACEVTLQPIDEFGFDAAILFSDILITLPPMGVDVAFPDAGPKIGAPVRNRA
                     AIDALHVPDPVAELPYTLEAIKQIKKGLNGRVPLIGFAGAPLTMLTYAVEGGGSKDYA
                     HTKKLLYGAPADAHALLDKLAQTCAVFLEAQVAAGVDCVQIFDSWGGIVSPGDFREYP
                     LRYAKQVIAHLRTKCPNTPIIYFVNGCAPYLDDYASSGADVLGVDWRVGIDEVRRRVG
                     DGVALQGNLDPGALFASPADIRARVADILAKAGPVGHIFNLGHGVLPETNPEHVRAMV
                     EAVKELSAQA"
     gene            2980..4026
                     /locus_tag="VMZ53_05880"
     CDS             2980..4026
                     /locus_tag="VMZ53_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS28014.1"
                     /translation="MTSDPSAIVLAPDGVMGSLVQLVERLRAANIQVEVADEVGLAAR
                     LASRTPFAPPAVLVDLRGLVQGAELEDIQVAAERLRKVVHAMPLVQPIAVTGNADARI
                     VVACIRAGAADVLDINLEGTAAARGVVARVYLKQLAWASHAHTAAALRGMIDDMLKDI
                     IRTERRSIDLEEKLAQFESTTGEVRELGDQRPPSILLVEPAVDIAKQLADRLQPAGVA
                     TYAFISGEDATRELDGLLGAGAGFDLALVAAQLPGIDGLETVRRLRDRLPGLPAFLMT
                     SIHDADLAARAADLGVVGFVQKPLPDLDEVVARLAQLAHDALERTREQLYLQRIKVRH
                     ERVLARYRSLPREQ"
     gene            4054..5412
                     /locus_tag="VMZ53_05885"
     CDS             4054..5412
                     /locus_tag="VMZ53_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HUS28015.1"
                     /translation="MSGAALSIKLRCASWQQLATIYKRDLSRGSMFLKASTQPAIGTI
                     VKIDLELPSATVIELTGTIVKHVTDPQRGTGVDLQLEPLPTKSVWMIENALAAETKVR
                     ATSQSDTNVAAQQAPHRAPTLTDGEDISAAEVELIRALEAEAESLKKLNPFLVLGVGY
                     ETSDADVRHAFGELTKRYHPDRFARYQSPKLRQIAAEIFILIRDAYRRLADDASRQQV
                     IATLGRVPTRAQTQPRGSGPQPPRITPPRIAVPAAIPPIPPKSSMVPDIPTTPGQPIT
                     QQHHDAPTNPSGKSAELAHTELARAEQNLAIPVEAQKAESGKFRKPVQTPPGGFAATD
                     KLPAKRPTASPVVEPVDTTAVDNLIDEGKLDEALNAYRTLAKKHPQDRSCRAGIELVE
                     GLKALAARDRLEAAQRFEAALEIDPSNERAARELAEMRRQATNERKGLLSRLMGKKEP
                     LP"
     gene            5409..7073
                     /locus_tag="VMZ53_05890"
     CDS             5409..7073
                     /locus_tag="VMZ53_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUS28016.1"
                     /translation="MTRIIGIDLGTTNSCVAILDEKGTPKTLVAADQERTTPSWVAWA
                     PDGTITVGTRARRQAVTNASATVYGAKRLIGRKVNAEDVAWFAKLAPFRIVAAPNGDA
                     WVRIQGQPISPQEVASHVLRRLRAVAEEALGEPVTRCVVTVPAYFDDAQRQATRDAGQ
                     IAGLDVVRILNEPTAAALAYGAHRVREGRRLIAVFDLGGGTFDISIMSVENGIFEVLA
                     TGGDSALGGEDWDRRILERIVDEVFDQYRVDLTQVPMALSRLRDAAETAKKALSASSE
                     TVIQLPFLANDQHGAPINYERPITRDEIEQLTQDLLDKVEAPCRRALKDAGLDAADVE
                     EVLLVGGMTRWPAVQRTVEKLFNRKPSKGANPDEVVALGAAAYAGILSGDADDAALLD
                     VTPHDIGIKVGDSGFSVMIPRNSMLPVRARKLFATASENQKFVSIELYQGESADLRGN
                     RKLGQVVLDDLPPGPPGSVRVELVMTVDVESMLGVTARELKSGKEASVTIRPSGGLSQ
                     REIVEIINRRRHESSFMGKLPSEGGTIARVATREQPAVMPPDDEKK"
     gene            7070..7597
                     /locus_tag="VMZ53_05895"
     CDS             7070..7597
                     /locus_tag="VMZ53_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUS28017.1"
                     /translation="MSKERVDPAVLKVADAVGALIEGWGFKRNMGRMWAVLYLEDHPL
                     TAAELGERLGLSTGAVSMLLTELLQWGIVKKAWVVGDRKDHYEPETSIWKMVSRVFRE
                     RELNWIKTAHEGFESAGKELSKGNDARRKLIAERIASLTQLAGVGATLLEAMLEGESI
                     DALPLKTIGDLAKKT"
     gene            7602..8609
                     /locus_tag="VMZ53_05900"
     CDS             7602..8609
                     /locus_tag="VMZ53_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS28018.1"
                     /translation="MIRAKDLCKHFKVHRRPPGFAAAMKSLVRRKYDTVKAVDGVSFT
                     IESGERVGFLGPNGAGKTTTLKVLSGLLHPTTGEVEVAGHVPFKRQPELLSKITLVMG
                     QKSQLIWDLPPAETYAMNRAIFDVDKAQFDKTLGELTELLELGPLVDKPTRQLSLGER
                     MKCELAAALLHRPTTLFLDEPTIGLDVAMQVAIRDFVRSYNQRHEATVLLTSHYMDDV
                     VALCPRIIVIDQGKLIHDGDLRKLIKTMDPDKRVSFALAQPVDQAELEKLGTILSREG
                     NRISLRVSERELPAVVNHVLTNLKAGDLAIEDPPLEDILRSMFGKSKEAHAPAAKPEA
                     S"
     gene            8606..9421
                     /locus_tag="VMZ53_05905"
     CDS             8606..9421
                     /locus_tag="VMZ53_05905"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HUS28019.1"
                     /translation="MKSTTSRTIRALPTLLRIGVAETVAYRAEFLVWMLTSTMPLINL
                     AFWTAVARDGQFQGYDAPRFTAYFLGVLIVRNLTGNWVAWQMSEEIRTGVLSMRLLRP
                     LHPFIAYASSQSAALPFRSLIAVPVAVILLVTAGAAITTDPVQLLLLLPSILLAWLIT
                     FGLMFALGCISFWITQTFGIVTFYFGMWTLFSGYMMPMDIMSLKFPLIAQIAKWLPFY
                     SMLGAPVEIMTKQLSTGEMLQIIGVQLGWAIVCIVVALRAWAAGIRKFEAVGG"
     gene            9418..10206
                     /locus_tag="VMZ53_05910"
     CDS             9418..10206
                     /locus_tag="VMZ53_05910"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HUS28020.1"
                     /translation="MRQYLRLVSVQLRTSVAQAMAYRADFLIQGVMSLAWLAVALIPL
                     LIAFDKRPTVAGWDRNSALVMMAFFMAVKAVMEGVISPSITNLVENIRSGAFDYILLK
                     PVDAQLMISASRYEPWRIFDLLGAIALATYAFIQIGHGPEPQHLAVGLALFVAGVFAV
                     YAVWICCAAASFWVVRLDNLVYLLQAIFDIARWPVQVFSPVWRLVFTFVIPVAVMTTF
                     PAMALLGRLSARTALATVGGSLLMLVISRLIWRSAIRNYTSASS"
     gene            10203..10787
                     /locus_tag="VMZ53_05915"
     CDS             10203..10787
                     /locus_tag="VMZ53_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28021.1"
                     /translation="MSALDSLQVLLVGAEPDADVIARPLAKGLFAKIVQAGEVDVTHD
                     DLAEWGLPEGELWERALGNLETDDAVIDSFVVEDTLTFFHVHGEGPFIASLALRLADL
                     GDNVPKHGWLFMVPHRRSLWFVPIGDHAHCMAGALIVERVADELEAADHVPIAGQIYW
                     TNGNRITAVPLAEVETGEDLTNAIIATVRAGNAS"
     gene            complement(10784..11920)
                     /locus_tag="VMZ53_05920"
     CDS             complement(10784..11920)
                     /locus_tag="VMZ53_05920"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HUS28022.1"
                     /translation="MTAEASDELTRVTLEAWLVTDVGVVRDHNEDSAHMEPSEGFFIV
                     ADGMGGHAAGEVASAMAVETVKQTLTAAKTEVEAFKKAPSDAGRRSIVQLLQNAVLQA
                     HQAVFQRGQTEQDKQGMGTTLDVVLVAGAEAFVAHVGDSRTYLVRDGKSSQITTDHTV
                     AEVLVIEGKLTIEEAQVSPLRTILVNAIGVSADVGVEMAHVTLKKGDRLLLCSDGLHD
                     YFPVEEEIAQRMSAEKPGEALKEMVELAKTRGGHDNITGVAVHVTEILEAAVPERVTG
                     DDSTQPVDVTGNPFAADEPTQTAAANALLGTAPSMPNLKQTQPMRIVDPTGPTVPPPL
                     QKPADETDGKPDGDAKADAKPADGVPAEEAATADTGQVKKPDAT"
     gene            complement(11996..12886)
                     /locus_tag="VMZ53_05925"
     CDS             complement(11996..12886)
                     /locus_tag="VMZ53_05925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HUS28023.1"
                     /translation="MERIEVSAPRRVEFGRVAPPDRLVHGDALQVASAEPTGSIDAIY
                     IDPPFGTGVIRQGRGLRYADRADDPTTFVQWLAPILAESHRTLAPHGSLFVHLDYRAV
                     HYVKVALDELFGRDLFVNEIIWCYSVGGKSRRGFGRKHDTILWYARSEDWAFFPDAVR
                     VPRRGGSHMRLIDGVQEKTDRKTGRVYRYPVHAGKVPEDWWTDVETLNHSDKERTGYP
                     SQKPLRLVERILHAVTQPGDLVADWFAGSGTTPTAAQRLGRRFLAVDREADSIAVCTK
                     RLVAQARTLSSPPPPLEIVG"
     gene            12909..13244
                     /locus_tag="VMZ53_05930"
     CDS             12909..13244
                     /locus_tag="VMZ53_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28024.1"
                     /translation="MRFSRWYPLAEAAAHAPAGEGVLQLRQATGLIEYPRGKSAMVHY
                     EHAADVRALATVLAAAHAGEDLWCRHLEIEEGEAVDAAAFHAKVREEFVRRFGSPPAF
                     PCISSKPKL"
     gene            13307..13762
                     /locus_tag="VMZ53_05935"
     CDS             13307..13762
                     /locus_tag="VMZ53_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28025.1"
                     /translation="MSFLFTMSILIGTVVAIYMLLPARDGLLITEAISKHRDSGPWEI
                     ENPSNETLRGWAIAVVGKEAPLPPAGSHVIGARQLTILKRRAALIRLQLGDDQVTYLV
                     QHARGIMPDHSSRTDGELRAEQRKRGKFTIVGVGPAATDKTWTRLLFGP"
     gene            complement(13748..14389)
                     /locus_tag="VMZ53_05940"
     CDS             complement(13748..14389)
                     /locus_tag="VMZ53_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28026.1"
                     /translation="MSATSCITPAQPISWLRLETFAIDRRDAAVKDHVAACPACKACL
                     DEIERDVVALPPLVVPVAREKKPRWWRFAIPVGVALAAAAALLLVIRPRDESLRSNET
                     RVKGVGIVVVDVVRERSGTIRDDVRTFRDGDRFKVVVTCPPDRSAHFEVRVREPGAQT
                     IDRPLQPVDLVCGNRVVMPGAFTLTGTNANEVCVDVTGPQGETKTACVTLRPE"
     gene            complement(14386..14874)
                     /locus_tag="VMZ53_05945"
     CDS             complement(14386..14874)
                     /locus_tag="VMZ53_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS28027.1"
                     /translation="MEPASDPQAAYRAFGPALVRKAERVLRSREDAVDVVHGLFADLI
                     PRWNKDVDLPYLYRAVTNRCLNMVRDRNTRARLLEREGTAAAPVARVKLEDQVVGVGL
                     IAALAEKLDESHMEVLVARFVDDMTQEEIAEHLGLSRKTIGKRLDRIRDEVLALRQEA
                     AG"
     gene            complement(14858..15958)
                     /locus_tag="VMZ53_05950"
     CDS             complement(14858..15958)
                     /locus_tag="VMZ53_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28028.1"
                     /translation="MRKHSLLFTIVIASQLGATKCGQVLRDPGFDLWCGEQLCAWKLE
                     RGEVKRVPTWHEGDGGVELVGTDVAIEQLTPVNSGDDTCIKFDLVANVDENAEVYLDV
                     DVQGDGSIEMHERIPTSHWEPLSYNIRVAPPYDGIRFELTKKGTGAAVLANIGANTAH
                     DQCAGLTALDPGPRRDGAQCDPTMASQCASGICRGSATEVPASAFGLACAGCNPAAPS
                     CGGTDVCGLGDAFSPVFAVPTQCVGTETKELGEKCITSAECASGGCFKLASDGMGVCS
                     TCFADNQCGGTACAPAWERGPLVCGAGQHVGAAGAPCGADEDCASGHCNGTVRRECDD
                     GRTCIDASQCPFESGLKNGPCTTVGVQGGTCQ"
     gene            complement(15967..17598)
                     /locus_tag="VMZ53_05955"
     CDS             complement(15967..17598)
                     /locus_tag="VMZ53_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HUS28029.1"
                     /translation="MRLVCFLLATLAFGAVASADDGIATYAIVVGSNAGGPGQTDLRY
                     AEDDARRVGKLLTELGGYQVDQVDIVVHPTPDELRARINKLAEKLAADEKAGRPTRVL
                     FYYSGHAKASAVNLGKEELPLPELRQKLFELPSKLTVVVLDACQSGAFSRVKGAAPAA
                     DFSFNSRQQLDATGVAVLASSSGSELSQESEQLKSSYFTHHLLVGLRGAGDNNGDGQV
                     TVDEAYRYAYHQTLLATAETAVGGQHVSLEVDLKGHGEVPLSYPKAATAHIELPAALE
                     GKTLIEDKRAHTVVAETYKAKGTAVRVAVAPGEYRILVRDGSTMSRCDVSAGGVVDLS
                     KCTRESLVEGTAKGGTHERQSRTDYQFLVGAERHDAFTDRLEEFGYNEQLGPAWGMQL
                     SDVYRIGRYGWVGGALITGRSSEWRRDTDGLYPLRFQWDTLTLGPTGRLETRLGSGGY
                     SSHFKAFAQLTLGLGLARTTFRDQTDDTTHEIYVGPAGAFGTGLLIGGDENRWRGFGF
                     SLGYQYAYARVVDNLIGDTHASGGGRLTLGVSLSH"
     gene            complement(17641..18840)
                     /gene="dinB"
                     /locus_tag="VMZ53_05960"
     CDS             complement(17641..18840)
                     /gene="dinB"
                     /locus_tag="VMZ53_05960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HUS28030.1"
                     /translation="MRTILHVDLDAFYAAVEQRDDPKLRGKPVLVGGTVRRGVVASCS
                     YEARKFGIRSAMPMAEAMRRCPHAIVVRHRMERYAEASELFFKILGDYSPEVEGLSLD
                     EAFLDVTGSERLLGDGKTIGETIKRRVRAELSLVASVGVAPIKLAAKIASDIDKPDGL
                     RVITPENLLPFLHGLPVTRLWGVGEATRTVLASMGLSTIGDVARYPEAALVARLGAVT
                     GHHLAALARGEDHRPVISEHEQVSIGHQETFDDDVDDKGELSVLLLDQADRVAARLRD
                     SHLRARAVVLIIKYDDFRQITRRTTLDAATSDGGLLARTAIELLAKVPIEDKKGSRVR
                     LCGIAASSLEPRDAPRQLLFDEATRQKGERLGDTIDKVAAKFGKSAIKRAIHVSKSSA
                     DGGDDDS"
     gene            complement(18840..19283)
                     /locus_tag="VMZ53_05965"
     CDS             complement(18840..19283)
                     /locus_tag="VMZ53_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HUS28031.1"
                     /translation="MSAQVTAWLLAAHLIGVFLWIGGLMTVYWILRIHTQTPPAIHEK
                     LVLMERALALTMDLAAALAIGCGLAMAIGNKLFSDKHYGWLHIKLAVVVLGILSVHGI
                     VRARVGRFSRGEMKPVPAWAWNVLIAALVAILILVTRVKLAYMMS"
     gene            19400..20362
                     /locus_tag="VMZ53_05970"
     CDS             19400..20362
                     /locus_tag="VMZ53_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28032.1"
                     /translation="MARVLKIFFHDACFDGTTSAALFSAFYRDAIAKDVQIQPIGMIH
                     RDGNPFEGIALDADDHACVDFRFCGDRRMKWWFDHHPTAFQPESLREIFDREHLPTWF
                     FDPSSPSCAGLIARTLEEKYKWTPPPHLVDAVSWADTIDAAQFASAEAAIALRHPAQR
                     LAAWLAHGRSMHDTANYVEWLSRASLSEVAQRPELAPQLAAVEAERTKELDDVRKLGV
                     WHDDVVVFDRFDDVGARSPGFLGYLLFPECLYAISGTRTPSSIKISVGVNPWTNKTRR
                     HDIGELCQRHGGGGHAVVGGITLRGDELSRARDTLKLLVRELTT"
     gene            complement(20366..21346)
                     /locus_tag="VMZ53_05975"
     CDS             complement(20366..21346)
                     /locus_tag="VMZ53_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28033.1"
                     /translation="MRWTSVLLVLGSAAIAHATPQQADVLKGEAEAAVKQNDFVTAAA
                     KFKAAYAADPRPDLICNVGVAYFKAKDLPRAQMFLSRCLERGSALDAKFVDNVRAVLG
                     SVETTLRAGDFTPVDIVVEPGGASVTIDSFGADESFVGARVVWLPKGTHQLKAAAEGY
                     VERAQTIEATGHDRMTLQIVLAKQPAVVEPPPVGSGAEQGSGAGAGSGSAIVVAPPPA
                     IVQPPKRSLVAPIVATAGTAAALAVAIVARGKAFDASDRAKLALNNSYYDADVSKIHT
                     WNAVFVTGVAVSVAGAGLSAYLWYRAFDTPSHVEVQPASGGGVVSLSGRW"
     gene            21378..22466
                     /locus_tag="VMZ53_05980"
     CDS             21378..22466
                     /locus_tag="VMZ53_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28034.1"
                     /translation="MRWPVVLSVLGACAPVPNPPSFALDVAQVDNNGESINLRFGELG
                     MQLAPMTSIQLPLVLTPDGGATNLLEPPPATNPCGEGYAGINLNPIARASASTMIGGS
                     TVRQLLSGPAVARVAVEYTVPYMCNGNRELKGKTTFTLLPSGRIERLDENVVASMTNV
                     PDTASCGCATAPDNAIYYSSFWSFKPGTDKTPTDTTRGSETGPGCTIEASGDTIAVDY
                     HNSEQRGLNGTHLVYFTTSVPPLTKIEPQTAQSSLVIGKNLRCADALARLEDPPIMVD
                     DKMVTAKQGIYVDTVNHASRAEITTQFPVLHGFALEIPLNGATHARVLKNGDPFPFAV
                     ETDGDNPVFWFPDPLVLGDTIVIDQFSE"
     gene            complement(22467..23432)
                     /locus_tag="VMZ53_05985"
     CDS             complement(22467..23432)
                     /locus_tag="VMZ53_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28035.1"
                     /translation="MRGAAAVMLVAMCVSRGDADPADDAVAEAQRKAAANDFPGAAAK
                     YREAFAAAPRPDLLCNVGVAYYKARDLPRAQRYLAHCLEIGTSLDRPFIDSVKKVLDA
                     VNDMLTRGDYTPVDVLVQPTTASFTANAVYDEAFVGSRRVWFPFGEYKLTVHAEGHVD
                     TIVEVNAETRTPVAARVTLERAPVVEKPPAGSGSGSAIASEGSPVEAPPPAILINPIR
                     KNRVVPIVVTTASFVAVGAALACYSLAKAKALTAPDAQSMEEWQDIADGVHLRENIAW
                     GFAGLAVAGAAVSGYLWYRVTRTETSHLEVTSNGDGATFVLTGRW"
     gene            23463..24755
                     /locus_tag="VMZ53_05990"
     CDS             23463..24755
                     /locus_tag="VMZ53_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28036.1"
                     /translation="MKRRLVWGALFGAVLGACISIPPYAQKDAGGDGAPTDGSGSGTP
                     ATVTYMDDGSGGGVVVGPFFNMHFAGGTKPHYPDAISIDNLPVLGHSTSAACAAEDEH
                     GVAFYPSGRVSPNGPANVSEGTMLDVVWRGPAIVKVIAETNVRIDGSDSSANAVTRHP
                     TITSRYTIHPDGKVFRYDTIEGGTGAAITPTSMSCFGGTPGQFIATSFSTWQREAGSH
                     LYVHSGTTAIDTPVPEFNMGATDVQNRTSACLDYGQRQVAFGWYDLNSRIRAPTADTL
                     AFVYDYALTAESEMGAADYWTRSMFMLGRGNSCTTDLPGIYSQVRAWEGRREMLNING
                     LGVEIANDGIYGGEADPGSTPGIWYGGDPVTLTGTISIPFAVWLIMSPAADDIVVEKA
                     DAQGPFYQKQRDASGTNWVIWFRDPLSSGQSITVRRAP"
     gene            24752..26302
                     /locus_tag="VMZ53_05995"
     CDS             24752..26302
                     /locus_tag="VMZ53_05995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28037.1"
                     /translation="MTKPMDPTMPSASDLAETQVHAEEAYCSACNQSYPAGTLTCPKD
                     GARLIKLAAETDTLIGRVLDQRYEIRSQLGKGGMGSVYRGWQLSVDREVAIKVIHPKL
                     SNDRSAVKRFLREARLASRLSQPNIVNVYDFGQSEGVLYLVMELLRGHTLASELGKGR
                     RLNPKRTVTIASQLCDALEAAHAQGIVHRDLKPSNIVILDDPPGRDLIKVLDFGLAKS
                     LKQDSGSLVTNTDALLGTPLYMAPEQIDGRESDQRADLYSLGCMLYEMLSGRPPFVDN
                     AVSAVLARHMHDTHVELPPHVPVRLRSMIDQLLVKKPEDRLQSAGDVRKILEDVRDSA
                     PVVAIPMSPGMSMDIPDTIPDVSPALAETFAATPVPESQVLDSAPKSRKSTPRIDTST
                     DQVKRPQPRRVAIFMAIAIVPIAVVALLVVKSMNNRASTGETGGAGNGSVGSSSGSGA
                     PQRLAVPQDAALAHPPPVDATEIDARGASTTPDAGRHRPRVVPPTSKVDAGATEPNID
                     FYEEKPKN"
     gene            complement(26299..>27257)
                     /locus_tag="VMZ53_06000"
     CDS             complement(26299..>27257)
                     /locus_tag="VMZ53_06000"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS28038.1"
                     /translation="KPEPVWIAELAGATLAIDRGAAAIELAKRGGPAAEAALVTALEK
                     DAFWAVRGTAALSLGKLRTPAARDRLIKRLKAEVNPRARRSIARALGDFVHDGTAAAA
                     LAAIVDKGDASYFVEAEACLALGRTRTGRAGELLRHAATRESFTDVIRQHAYRGLAEA
                     RDDSALGLLVDGLKWGRPTQGRRAAAGALAILMRGRRDREARDVRERIELLLVDKDFR
                     VQAAAMEALFVIGDPGAIPALRRMIDRELDGRLRRRGKEIVRDLGEGAPLAEDVRRLR
                     DEVGELRNQVGTLRERIEMMEARNAPAVKKPASKRAPKKKRR"
BASE COUNT         4544 a         8992 c         9117 g         4604 t
ORIGIN      
        1 cggcgcctgc tttcgtgatg acgaccttcg caggcgattc cgcagcactc gcgacgatga
       61 gcttgtcgct tgcgagggcg acacgcggcg tgagcgcgca gtgatcgagg acgacgcgcg
      121 cttcgcgcgt cattgcgcgc gcagtcatcg cgggcgcggt cgcggagccg gtcggcgcgg
      181 agccggtcgt cgcgcgggtc ccgttcgatc cgctcgagct cgcggcttgt ggcgcgggcg
      241 gcgcatcgat catcacgaac acctcgggga tgccccacgt tgtggttggt gcgacggacg
      301 cggcgcgcgg ggtgttgcaa ggcgtgcggc ccgacgaggc ccgcgcggcg agcggcacgt
      361 ccttccactc gacgcggagc tgcacatcgc ctttgcccga cggtggcggc gcagtcggat
      421 cggtgacggg ggcggagtct tgcggatgct tgcgcgcgtc ctcgacgcct ttcgtcttgc
      481 ccgagcaccc gccgagcagc gcgagcacga ggagagcatt cgatgaacga acgttcattc
      541 actcagctcc acgtgcgcag ctccgagacg atgacgtcgc ggtcggcgca gagatcgttg
      601 acgccggcgc cgcggtagtc cgcgcctgcg agcgcgatgc ggtggacgcg cccagcagcg
      661 accgcggcgc ggacgtaatc gcggtggccg accggatatt gcgcgatacc gtgcgaccag
      721 cggatgacac tcgcgtgcgt gggggcgggc agcgttgtca tcgcgccgcg cgatttcttc
      781 gtcgacgcga acgccttggc gacgtcgcgg agcgcgtgct cgatcagctc ggcgtcggtg
      841 agcttcgtcg catcgggatc ccgaccgccg ccgaagatgc agcgcaggag tgcttggcct
      901 tctggcgcgc ggttgctcca gaccgtcgac tcgaaaacga cgccgagcac gcgcagatcc
      961 tcgccttgag cggtgaggaa gccaaagccg tcggaggcac gcggcaccga gtcggcgggg
     1021 aggccgagat agacgagggc gaccggcgcg cgctcgaaca cgcgcagccg cgacgcgagc
     1081 tcgggcattg cgtcgtcgac gaggctcgca ccttcgtgtg cgggtgttgc gagcacgacc
     1141 gcgtcccagc gttcgttgtc gaccagaacg cccgcggtcg ctggggccgg agcgatcgtc
     1201 cggatccgac ggtgcgtgcg gatgcgcggg ccgagcttcg tcgtgagcgc ttcgacgagc
     1261 gagccgaggc cgcccgccgg tgcccacatt cccgatggtg ttttggcggt ccgctttccg
     1321 gacagcttga cgagaaggct cttgcctacc tgcttggcgg cgctgcgcac gacgccgcct
     1381 tctgcgtcga attgggcgag gcgagggaag ccggcttcga ggctgatggc gtgcgagtcg
     1441 gcggcgaaga tgccggtcac gaacggtgcc acgatcgcgc gtgcggcctc cgcgccgaag
     1501 cgtcgagccg cgaacgcatg gatcgattcg tcctcggtgc ccttgcgtgg agcgagcggc
     1561 tcgcggagca gcgcgagctt gccgcgccac gtgagtagat cgctgcggag gaagtcgatc
     1621 gggttgcgcg gaagagcgcg cagcttgccg tcgatgtaga tccagcgcgt cttcgcgcgt
     1681 ggcgacgcgg cttcgacttc gatgccgagc tcttggcata gcgcgagcgc gccgcgcgat
     1741 gggccgccga gaaacgagct cgctgcgtgc tcgcggacga agccgtcgac gatgctcgtg
     1801 ccgatcacgc cgcccggcgc tggcgcggct tctagcacgt gcgcatcgaa gcccgcgctg
     1861 acgagaccgt gtgcggccac gagaccgccg agaccgccgc cgacaacagc gactctcatg
     1921 cctgtgcgct cagctccttc acagcctcga ccatcgcgcg cacgtgctcg ggattcgtct
     1981 ccgggaggac gccgtgaccg agattgaaga tgtggccgac ggggcctgcc ttcgcgagga
     2041 tgtcggcgac gcgcgcgcgg atatcggcgg gcgacgcgaa cagcgcgcct ggatcgaggt
     2101 tgccttgcag cgccacacca tctccaacac gccggcgaac ctcatcgatt cccacgcgcc
     2161 agtcgacgcc gagaacgtcg gcgccactcg atgcgtaatc gtcgaggtac ggcgcgcagc
     2221 cgttgacgaa atagatgatc ggcgtgttcg ggcacttcgt gcgcaggtgc gcgatgactt
     2281 gcttggcgta gcgcagcggg tattcgcgga agtcacccgg cgagacgatg ccgccccagc
     2341 tatcgaagat ctgcacgcag tcgacgcccg ctgcgacctg cgcctcgagg aagaccgcac
     2401 acgtctgcgc gagcttgtcg aggagagcgt gcgcatcggc cggcgctccg tagagcagct
     2461 tcttcgtgtg cgcgtagtcc ttcgagccac cgccttcgac ggcgtacgtg agcatcgtga
     2521 gcggcgcgcc ggcaaagccg atgagcggta cgcgaccgtt gaggcccttc ttgatctgct
     2581 tgatcgcttc gagcgtgtac ggcagctccg cgactggatc ggggacgtgc agcgcatcga
     2641 tcgcagcgcg gttgcgaact ggcgcgccga tcttcggacc ggcgtcgggg aacgcgacgt
     2701 cgacgcccat cggcggcagc gtgatgagaa tgtcggagaa caagatcgcc gcgtcgaagc
     2761 caaactcgtc gatcggctgc agcgtgacct cgcatgcgag ctcgggcgtg cggcacaggt
     2821 ccaggaacga cgccttggcg cgcacggccc gataggcggg caagtaacga cccgcttgcc
     2881 gcatcatcca gatcggcgtg cgagggacag gctgccggcg gcacgcacgc aggaacagat
     2941 gctcggacat cgcgcttatc ctaggttatc gtccgttgcg tgacgagcga cccgagcgcg
     3001 atcgtgctcg caccggatgg cgtgatgggg tcgctggtgc agctcgtcga gcgcctgcgg
     3061 gccgcaaata tccaggtcga ggtcgccgat gaagtgggct tggccgccag gctcgctagc
     3121 cgcacaccgt tcgcgccgcc tgcggtcctc gtcgacttgc gcgggctcgt gcaaggcgca
     3181 gagctcgagg acatccaagt cgcggccgag cgcctgcgca aggtcgtcca tgccatgccg
     3241 ctcgtgcagc cgattgcggt gaccggcaac gcagatgcgc gcatcgtggt cgcctgcatc
     3301 cgcgccggtg ctgcggacgt gctcgatatc aatctggagg gcaccgctgc tgcgcgcggc
     3361 gtcgtcgcac gcgtctatct aaagcagctg gcgtgggcga gccacgctca tactgcagcg
     3421 gcactgcgcg ggatgatcga cgacatgctc aaggacatca ttcgcaccga gcgccgctcg
     3481 atcgatctcg aggaaaagct cgcccagttc gagagcacga ccggcgaggt gcgcgagctc
     3541 ggcgatcagc gcccgccctc gatcttgctc gtcgagcccg cggtcgatat cgcgaagcag
     3601 cttgccgacc ggctgcagcc ggcgggagtt gcgacgtacg cgttcatctc gggcgaagac
     3661 gcgacgcgcg agctcgatgg cctcctcggg gcgggcgcgg gcttcgactt ggcattggtg
     3721 gccgcgcagc tgcctggaat tgatggcctg gagacggtcc gccgccttcg cgatcggttg
     3781 cctggcctac cggcatttct catgacctcc atccacgatg ccgatctcgc tgcgcgcgcc
     3841 gcagacctcg gtgtcgttgg tttcgtgcaa aagccgctgc cagacctcga cgaggtcgtc
     3901 gcgcgcctcg cccagctggc acatgacgcg ctcgaacgca cgcgtgagca gctgtatttg
     3961 cagcgaatca aagtccgtca cgagagagtg cttgcacggt accgatcgtt gcctcgcgaa
     4021 cagtgaatcg cctatgattt cgcggattcg tcgatgtcgg gggctgcact cagcatcaag
     4081 cttcgttgcg cgtcctggca gcagctagcg accatctaca agcgcgacct cagtcgcggg
     4141 tcgatgttcc taaaggcgtc cacgcagccg gcgatcggta cgatcgtaaa gatcgatctc
     4201 gagctgccga gcgcgacggt catcgagctc acagggacga tcgtcaagca cgtcacggat
     4261 ccgcagcgcg gcaccggcgt ggacctccag ctcgagccgc tgccgacgaa gtcggtctgg
     4321 atgatcgaga acgcgctcgc cgccgagacg aaagtccgcg cgacaagcca gagcgatacc
     4381 aacgtcgcgg ctcaacaagc gccgcatcgc gcaccgacgc tcaccgatgg cgaggacatc
     4441 tcggccgccg aggtggagct gatccgagcg ctcgaagccg aggccgagtc gctgaagaag
     4501 ctgaatccgt tcctcgtgct gggcgttggc tacgagacct cggacgccga tgtccgccat
     4561 gcatttggcg agctgacgaa gcgctatcac ccggatcggt ttgcgcgcta tcagtcgccc
     4621 aagctgcggc agatcgcggc ggaaatcttc attctgattc gcgatgcgta tcgccgtctc
     4681 gcggacgacg cgagccgcca gcaagtgatc gcaacgctcg gccgcgttcc gacgcgcgcg
     4741 cagacgcagc cgcgtggttc gggaccgcag ccgccgcgga ttactccgcc gcgcattgcc
     4801 gtgcccgccg cgattccgcc gattccaccg aagtcgtcga tggtgccgga catcccgacg
     4861 acaccgggcc agccgatcac gcagcagcac cacgacgcgc cgacgaatcc gagcggcaag
     4921 agcgcagagc tcgcgcacac cgagctcgcc cgtgcggagc agaacctcgc gatcccggtc
     4981 gaggcgcaga aggcggagag cggcaagttc cgcaagcccg tgcagacgcc gccgggcggc
     5041 ttcgctgcga cggacaagct tcccgcgaag cggccgaccg catcgcccgt cgtcgagccc
     5101 gtcgacacga ccgccgtcga caatttgatc gacgagggca agctcgacga agcgctcaac
     5161 gcctatcgca cgctcgcgaa gaagcatcca caagatcgct cgtgccgcgc gggcatcgag
     5221 cttgtcgaag gcttgaaggc gctcgccgcg cgcgatcgac tcgaagcagc acagcgcttc
     5281 gaagcagcac tcgagatcga tccgtcgaac gagcgcgccg cgcgagagct cgccgagatg
     5341 cggcgccaag caaccaacga acgcaagggg ctgctgtcgc gcctcatggg caagaaggag
     5401 ccgctgccat gactcggatc atcggcatcg atctcggtac gaccaactcg tgcgtcgcaa
     5461 ttctcgacga gaagggcacg ccgaagacgc tcgtcgccgc ggaccaagag cgcacgaccc
     5521 catcgtgggt ggcctgggcg ccggacggca cgatcaccgt cggcacgcgc gcgcgtcgtc
     5581 aggcggtcac gaatgcatcg gcgaccgtct acggcgcgaa gcgattgatc ggccgcaagg
     5641 tgaacgccga ggacgtggcg tggttcgcga agctcgcgcc attcaggatc gttgcagcgc
     5701 cgaacggcga cgcatgggtg cgcattcaag gccagccgat cagcccgcag gaagttgcat
     5761 cgcacgtgct gcggcggctg cgcgcggtgg ccgaagaagc gctcggcgag ccggtcacgc
     5821 gctgcgtggt gacagtgccc gcgtacttcg acgatgcgca gcggcaagcg acacgggacg
     5881 ccggccagat cgcgggcctc gatgtcgttc gcatcctcaa cgagccgacc gcagcggcgc
     5941 tcgcgtatgg ggcgcatcgc gtgcgagagg gcaggcgcct gatcgcggtg ttcgatctcg
     6001 gcggcggcac gttcgacatc tcgatcatgt ctgtcgagaa cggcatcttc gaggtgctcg
     6061 caaccggtgg cgacagcgcg ctcggcggcg aagactggga ccgccgcatc ctcgagcgaa
     6121 tcgtcgacga agtcttcgat caataccgcg tcgatctcac gcaggtgccg atggctctct
     6181 ctcgcctgcg cgatgccgca gagactgcga aaaaagcact ctcggcatcg agcgagacgg
     6241 tgatccagct gccgttcctc gcgaacgatc agcacggtgc tccgatcaac tacgagcggc
     6301 cgatcacgcg cgacgagatc gagcagctca cacaagactt gctcgacaag gtcgaagcgc
     6361 catgtcgtcg cgcgctgaaa gacgccgggc tcgacgccgc ggatgtcgaa gaggttctgc
     6421 tcgtcggcgg tatgacgcgg tggccggccg ttcagcgcac cgtcgagaag ctgttcaatc
     6481 gcaagccgag caagggagcg aaccccgacg aggtggtcgc gctcggtgcg gctgcgtacg
     6541 ccggcattct gtccggtgat gccgacgacg ccgcgctgct cgacgtcacg ccgcacgaca
     6601 tcggcatcaa agttggcgat agcggattct ccgtgatgat cccgcgcaac tcgatgctgc
     6661 ccgtgcgcgc gcgcaagcta ttcgcgacag cgtccgagaa tcagaagttc gtctcgatcg
     6721 agctgtatca aggcgagtcc gcggatctgc gcggcaaccg caagctcggc caagtcgtgc
     6781 tcgacgatct gccgccgggc ccgccgggca gcgtgcgtgt cgagctggtg atgacggtcg
     6841 atgtcgagtc gatgctcggc gtcaccgcgc gcgagctcaa gagtggcaag gaagcatcgg
     6901 tcacgattcg tccgtcgggc ggactctcgc agcgcgagat cgtcgagatc atcaatcggc
     6961 ggcggcacga gagctcgttc atgggcaagt tgccgagcga gggtgggacg attgcccgcg
     7021 tcgcgacgcg cgagcagccg gcggtcatgc cgcccgatga cgagaagaaa tgagcaagga
     7081 gcgcgtcgac ccggcggtcc tcaaggttgc cgatgcggtc ggtgccctga tcgagggctg
     7141 gggcttcaag cgcaacatgg gccgcatgtg ggcggtgctc tatctcgagg atcatccact
     7201 gaccgccgcg gagctcggcg agcgcttggg gctctcgacc ggcgcggtgt cgatgctgct
     7261 caccgagctg ctgcagtggg gcatcgtgaa gaaggcgtgg gtcgtcggcg atcgcaagga
     7321 tcactacgag cccgagacgt cgatctggaa gatggtgtcg cgcgtgttcc gcgagcgcga
     7381 gctcaactgg atcaagaccg cgcacgaagg cttcgagtcg gcaggcaaag agctgagcaa
     7441 aggcaacgac gctcgccgca agctgatcgc cgagcgcatc gcgagcctca cccagctcgc
     7501 cggcgtcggt gccacgttgc tcgaggccat gctagaaggc gagtcgatcg acgcgttgcc
     7561 gctcaagacg atcggcgacc tcgccaagaa aacctgagcc gatgattcgc gcgaaggatc
     7621 tatgcaagca cttcaaggtc caccgccggc cccccggctt tgcggccgcg atgaagtcgc
     7681 tcgtgcgtcg caagtacgac acggtgaaag ccgtcgacgg ggtgagcttc acgatcgaga
     7741 gcggtgagcg cgtcggcttc ctcggcccga acggtgccgg caagacgaca acgctgaagg
     7801 tcctctctgg gctgctccat ccgacgacgg gcgaggtcga agtcgccggt cacgtgccat
     7861 tcaagcgcca gcccgagctc ctctcgaaga tcacgctcgt gatggggcag aagtcgcagc
     7921 tcatctggga cctgccgccc gccgagacgt acgcgatgaa ccgcgcgatc ttcgacgtcg
     7981 acaaagcgca attcgacaag acgctcggcg agctgacgga gctgctcgag ctcggcccac
     8041 tcgtcgacaa gcctacgcgc cagctctcgc tcggcgaacg catgaagtgc gagctggccg
     8101 cggcgctgct gcaccgcccg acgacgctgt tcctcgacga gccgacgatc ggtctcgatg
     8161 tcgcgatgca ggtcgcgatt cgcgacttcg tgcgcagcta caaccagcgc cacgaagcga
     8221 cggtgctcct cacgagccac tacatggatg acgtcgtcgc gctgtgccca cgcatcatcg
     8281 tgatcgacca gggcaagctg atccacgacg gcgatctacg caagctgatc aagacgatgg
     8341 atcccgacaa gcgcgtgtcg ttcgctctcg cgcagcctgt cgatcaagcg gagctcgaga
     8401 agctcgggac gatcttgtcg cgcgagggca accggatctc tctccgtgtg agcgagcgcg
     8461 agctgcccgc cgtcgtcaat cacgtgctca cgaacctaaa ggcgggcgat ctcgcgatcg
     8521 aagatccgcc gctcgaggac atcctgcgct cgatgttcgg caagtcgaaa gaagcgcacg
     8581 cgcccgcggc caagcccgag gcgtcgtgaa gtcgacgacg agccggacga ttcgcgcgtt
     8641 gccgacgcta ctgcgcatcg gcgtcgccga gaccgtggcc tatcgcgctg agtttctcgt
     8701 ctggatgctc acgtcgacga tgccgctgat caacctcgcg ttctggacgg cggtcgcgcg
     8761 cgatggccag ttccagggct acgacgcgcc gcgcttcacc gcgtacttcc tcggcgtgct
     8821 gatcgtccgc aacctgacgg gcaactgggt cgcgtggcag atgtccgagg agattcgcac
     8881 cggcgtgctc tcgatgcgcc tgttgcgccc gctgcatccg ttcatcgcgt acgcgtcgag
     8941 ccaatctgcc gcactgccat tccgctcgct gatcgcagtg ccggtcgcgg tcattttgct
     9001 cgtcaccgcc ggcgcggcca tcacgaccga tccggtccag ctcctgctgc tcctgccgtc
     9061 gatcctgctc gcatggctga tcacgttcgg cctcatgttc gcgctcggct gcatctcgtt
     9121 ctggatcacg cagacgttcg gcatcgtcac gttctacttc gggatgtgga cactcttcag
     9181 cggctacatg atgccgatgg acatcatgtc gttgaagttc ccgctcatcg cgcagatcgc
     9241 gaagtggctg ccgttctact cgatgctggg ggcaccggtc gaaatcatga cgaagcagct
     9301 ttcgacggga gagatgctcc agatcatcgg cgtgcagctc gggtgggcga tcgtctgcat
     9361 cgtcgtcgcg ctccgcgcgt gggccgctgg catccgcaag ttcgaggctg tcggcggatg
     9421 aggcagtacc tccgactcgt gtccgtccag ctgcgcacgt cggtcgcgca agcgatggcg
     9481 tatcgcgcgg acttcttgat ccaaggcgtg atgtccctcg cgtggctcgc cgtcgcgctc
     9541 atcccgctgc tcatcgcgtt cgacaagcgg cctaccgtcg caggttggga tcgcaactcc
     9601 gcgctcgtca tgatggcgtt cttcatggcg gtgaaagcgg tgatggaggg cgtgatctcg
     9661 ccgtcgatca cgaacctcgt cgagaacatc cgctcgggcg cgttcgacta catcttgctc
     9721 aagcccgtcg acgcgcagct gatgatctcg gcgtcgcgct acgagccgtg gcgcatcttc
     9781 gatctgctcg gcgcgatcgc gctcgcaacc tacgcgttca tccagatcgg ccacgggccg
     9841 gagccgcaac acctcgccgt cggcctcgcg ctcttcgtgg caggcgtgtt cgcggtctac
     9901 gccgtctgga tctgctgcgc ggccgcgtcg ttttgggtcg ttcgcctcga taacctcgtc
     9961 tacctgctgc aagccatctt cgatatcgcg cgctggcccg tccaggtgtt cagccccgtg
    10021 tggcggctcg tgttcacgtt cgtcattccg gtcgccgtca tgacaacatt tcctgcgatg
    10081 gcgctcctcg gcaggctcag cgcgcgcacc gcactcgcta cggtcggcgg ctcgctgctc
    10141 atgctcgtca tcagccggct catctggcgc agcgcgatcc gcaactacac gagtgcctcg
    10201 tcgtgagcgc gctcgattcg ctgcaagtcc tcctcgtcgg tgcagagccc gacgccgatg
    10261 tgatcgcgcg cccgctcgca aaggggctat tcgcgaagat cgtgcaagca ggcgaagtcg
    10321 acgtcacgca cgacgacctc gcggagtggg gccttcccga gggagagctc tgggagcgtg
    10381 cgctcggaaa tctcgagacc gacgacgccg tcatcgactc gtttgtcgtc gaggacacgc
    10441 tcacgttctt ccatgtgcac ggcgaagggc cgttcatcgc gtcgctcgcg ctgcgcctcg
    10501 ctgacctcgg cgacaacgtg ccgaagcacg gctggctgtt catggtgccg caccgtcgct
    10561 cgctgtggtt cgtgccgatc ggcgatcacg cgcactgcat ggccggcgcc ctcatcgtcg
    10621 aacgcgtcgc cgacgagctc gaggctgccg accacgtgcc gatcgccggc caaatctatt
    10681 ggacgaacgg caaccgcatc acggccgtgc cgctcgcgga ggtcgagacc ggcgaggacc
    10741 tgacgaacgc gatcatcgcg acggtccgtg caggcaacgc cagctaggtc gcgtcgggct
    10801 tcttgacctg accggtgtcg gcggtcgcgg cttcttcagc cggcacgcca tcggctggct
    10861 tcgcgtcggc cttcgcgtcg ccgtccggct tcccgtcggt ttcgtctgca ggcttctgca
    10921 gcggtggcgg cacggtgggc cccgtcggat cgacgatgcg catcggctgc gtctgcttca
    10981 agttcggcat gctcggcgcg gtgccgagga gggcgttcgc ggcggcggtc tgcgtcggct
    11041 cgtcagcagc gaacggattg ccggtgacat cgacgggctg cgtggagtcg tcgccggtga
    11101 cgcgctcggg gacggcagct tcgaggatct cggtaacgtg gacggcgacg cccgtgatgt
    11161 tgtcgtggcc gccgcgcgtc ttcgcgagct cgaccatctc cttgagcgct tcgcccggtt
    11221 tctcggcgga catgcgctgc gcgatttctt cctcgaccgg gaagtagtcg tgcaggccgt
    11281 cgctgcagag caggagacga tcgcctttct tgagcgtgac gtgcgccatc tcgacgccga
    11341 cgtccgcgga cacaccgatc gcgttgacca gtatcgtccg tagcggcgag acctgggctt
    11401 cttcgatcgt cagcttgccc tcgatgacga gaacttcggc caccgtgtgg tccgtcgtga
    11461 tctgcgacga cttgccgtcg cggacgagat acgtgcggct gtcaccgacg tgcgcgacga
    11521 atgcttcggc gccggcgacg aggacgacgt cgagcgtcgt gcccatgcct tgtttgtctt
    11581 gctcggtctg cccgcgctgg aacacagctt ggtgagcttg gaggaccgcg ttctggagga
    11641 gctggacgat cgagcggcgg cccgcgtcgg agggcgcttt cttgaacgcc tcgacctcgg
    11701 tctttgcagc ggtcagcgtc tgtttcaccg tctcgacggc catcgcggac gcgacctcac
    11761 cggcggcgtg accacccatg ccgtcggcga cgatgaagaa accctcgctc ggctccatgt
    11821 gtgccgagtc ctcgttgtga tcgcggacga cgccgacgtc cgtgacgagc cacgcttcga
    11881 gcgtcacgcg ggtgagctcg tctgatgctt cggccgtcat cgtgtagctc cagaatcttc
    11941 acccgtggtg tcgcaggttc gcaagcgttc tggatggtta gaagctggat agacgtcagc
    12001 cgacaatctc gagcggcggt ggcggcgagc tgagcgtgcg ggcttgcgcg acgagacgct
    12061 tcgtgcacac ggcgatggaa tcggcttcgc gatcgacggc gaggaagcgg cggcccaggc
    12121 gctgcgcggc ggtgggcgtc gtgccggagc ccgcgaacca atcggccacg aggtcgccgg
    12181 gctgggtgac cgcgtggagg atgcgctcga cgagccgcag cggcttttgc gacgggtagc
    12241 cggtgcgctc cttgtcgctg tggttgagcg tctcgacgtc ggtccaccaa tcttcgggga
    12301 ccttgccggc atggactgga tagcgataga cgcgccccgt cttgcggtcg gtcttctcct
    12361 ggacgccatc gatgaggcgc atgtgcgagc cgcctcggcg aggtacgcga acggcatccg
    12421 ggaaaaacgc ccagtcctcg ctgcgcgcgt accagaggat cgtgtcgtgc ttgcgcccga
    12481 agccgcggcg cgatttgccg ccgaccgagt agcaccagat gatctcgttg acgaacagat
    12541 cgcggccgaa cagctcgtcg agcgcgacct tcacgtagtg aaccgcgcgg taatcgagat
    12601 gcacgaacag cgagccatgt ggcgcgagcg tgcgatgcga ctcggcgagg atcggcgcga
    12661 gccactgcac gaacgtggtg ggatcatcgg cgcggtccgc gtaccggagg ccacggcctt
    12721 gccggatcac gccggtgcca aagggcgggt cgatgtaaat cgcgtcgatc gatccggtcg
    12781 gctcggcgga ggcgacctgc aacgcgtctc cgtggacgag gcgatcggga ggcgcgacgc
    12841 ggccgaattc gacccgccgg ggcgccgaga cctcgattcg ttccacgccc ggaccttaca
    12901 ttagtggtgt gagattcagc cgctggtacc cgctggccga ggctgccgcg catgcgccgg
    12961 ccggcgaggg cgtgctccaa ctgcggcagg ccacgggcct catcgaatac ccgcggggca
    13021 agagcgcgat ggtccactac gagcacgccg cggatgtgcg cgctcttgcc acggtgctgg
    13081 cggcggccca tgccggcgag gacctgtggt gccgccactt ggagatcgaa gagggcgaag
    13141 ctgtcgacgc ggcggcgttc catgccaagg tacgtgaaga gttcgtacgg cgctttggtt
    13201 cgccgcccgc attcccatgc atctcgtcga agccgaagct gtagggccga tagaaggaga
    13261 ggatcccgtt gtcctcccac cgcgcccgcc acaccgccgc gtgtcggtga gcttcctgtt
    13321 cacgatgtcg atcctgatcg gcacggttgt cgcgatctac atgctcttgc cggcgcgcga
    13381 cggcctgctg atcacggaag cgatctcgaa gcatcgcgac agcggcccgt gggagatcga
    13441 gaatccgagc aacgagacgc tgcgtggttg ggcgatcgcg gtcgtcggca aagaggcgcc
    13501 gctgcctccc gccggctcgc acgtgatcgg cgcccgccag ctcacgatcc tcaagcgacg
    13561 cgccgcgctg attcgcctgc agctcggcga cgatcaggtc acgtatctgg tgcagcacgc
    13621 gcgcggcatc atgcccgatc actcgagccg gaccgacggc gagctccgcg ccgagcagcg
    13681 caagcgcggc aagttcacga tcgtcggcgt tggtcctgcc gcaacggaca agacgtggac
    13741 gcgcctgcta ttcgggccgt agcgtcacgc acgcggtttt cgtctcgccc tgcggtcccg
    13801 tgacgtcgac gcagacctcg ttcgcgttcg tgcccgtcag cgtgaacgcg cctggcatca
    13861 cgacgcggtt gccgcacacg agatcgacgg gctgcagcgg ccggtcgatc gtctgcgcac
    13921 cgggctctcg gacgcgcact tcgaagtgag cgctgcgatc cggcgggcag gtgacgacga
    13981 ccttgaagcg atcgccgtcg cggaacgtgc ggacatcgtc gcggatggtc ccgctccgct
    14041 cacgcacgac atcgacgacg acgatgccga cgcctttcac gcgggtttcg ttcgagcgga
    14101 gactttcgtc gcgtggcctg atgacgagca gcagcgcagc agccgcagcg agcgcaactc
    14161 cgacgggaat tgcgaaccgc caccaccgcg gcttcttctc gcgcgctact ggcaccacga
    14221 gcggcggcag cgcgacgaca tcgcgctcga tctcgtcgag gcacgccttg cacgcgggac
    14281 acgcggcaac gtggtctttc accgccgcat cgcgacgatc gatcgcgaac gtctcgagcc
    14341 gcagccagct gatcggctgc gctggagtga tgcacgacgt cgcgctcatc ccgcagcctc
    14401 ctggcgcagc gcgaggactt catcgcgaat gcgatcgagc cgcttgccga tcgtcttgcg
    14461 agagaggccg aggtgctcgg cgatctcttc ttgcgtcata tcgtcgacga atctcgcgac
    14521 gagcacttcc atgtggctct cgtcgagctt ctcggcaagt gcggcgatga ggcctacccc
    14581 gaccacctgg tcctcgagct tgacgcgtgc caccggtgcg gcggccgtgc cttctcgctc
    14641 gagcaggcgt gcgcgcgtgt tccgatcgcg aaccatgttg aggcagcgat tggtgacggc
    14701 gcgatagagg tacgggaggt ccacgtcttt gttccagcga gggatgagat ccgcgaacag
    14761 accgtgcacg acatcgacag cgtcctcgcg gctgcgcagc acgcgttcag ccttgcgcac
    14821 gagcgcgggg ccgaaggcgc gatacgccgc ctgaggatca ctggcaggtt ccaccttgga
    14881 cgccgactgt agtgcagggc ccgttcttca ggccgctctc gaacggacat tgcgatgcat
    14941 cgatgcacgt gcgtccgtcg tcgcactctc ggcgaactgt gccattgcag tgaccgctcg
    15001 cgcagtcctc gtcggcgccg cacggagcgc cggctgcccc gacatgctga cctgcgccac
    15061 acacgagcgg gccacgctcc cacgccgggg cgcatgccgt gccgccgcac tggttatcgg
    15121 cgaagcacgt cgagcacacg cccattccgt cgctcgcgag cttgaagcac ccgcccgatg
    15181 cgcactccgc gctcgtgatg catttctcgc cgagctcttt cgtttctgtg cccacgcatt
    15241 gcgtcggcac ggcaaacacc ggagagaacg catcgccgag cccacacacg tcggtgcccc
    15301 cgcagctcgg agccgcggga ttgcagccgg cgcacgccaa tccgaatgcc gaagcaggaa
    15361 cctcggtcgc actgccgcgg cagatgccgg atgcgcattg cgaggccatt gttggatcgc
    15421 attgcgcgcc atcgcgacgg gggccaggat cgagggcagt gagacccgcg cactgatcgt
    15481 gggccgtgtt cgcgccgatg ttcgcgagga ccgcagcgcc ggtacccttc ttcgtcaact
    15541 cgaagcgaat gccgtcgtac ggcggtgcga cacgaatgtt gtacgacagg ggctcccagt
    15601 gcgacgtcgg gatgcgctcg tgcatctcga tcgagccgtc gccttgtacg tccacatcga
    15661 gatagacctc ggcgttttca tcgacgtttg caacgagatc gaatttgatg caggtgtcgt
    15721 cgccggaatt gacgggcgta agctgctcga tcgcgacatc cgtgccgacg agctccacac
    15781 cgccatcacc ttcgtgccac gtcggcacgc gcttgacctc gccgcgctct agcttccacg
    15841 cgcagagctg ttcgccgcac cagagatcga accccggatc tcggaggacc tggccgcact
    15901 tcgtcgcgcc gagctgcgac gcgatgacga tcgtgaaaag gagggaatgc ttgcgcatga
    15961 catgcctcag tggctgagcg aaacaccaag cgtcaggcgt cctccgcccg atgcatgggt
    16021 gtcgccgatc aagttgtcga ccacgcgtgc gtatgcgtac tgatagccga gcgagaagcc
    16081 gaacccgcgc caacgattct cgtcgccgcc gatcagaagg cccgtgccga atgcgcctgc
    16141 gggaccgaca tagatctcgt gcgtcgtgtc gtcggtctga tcgcgaaacg tggtgcgagc
    16201 gagcccgagg ccgagcgtga gctgcgcgaa tgctttgaag tgcgacgagt agccgccaga
    16261 gccaagccgt gtctcgagac ggcccgtcgg accgagcgtg agcgtgtccc actggaagcg
    16321 cagcggatac aggccgtcgg tgtcacgccg ccactccgat gagcgccccg tgatcagcgc
    16381 accgccgacc catccgtagc gcccgatccg atagacgtcg gagagctgca tgccccatgc
    16441 gggaccgagc tgctcgttgt aaccgaattc ttccagccga tcggtgaacg catcgtggcg
    16501 ctctgcgccg acgaggaatt ggtaatcggt ccgagactgc cgctcgtgcg tgccgccctt
    16561 tgcggtgccc tcgacgagtg attcgcgcgt gcacttgctc aagtcgacga cgccgccggc
    16621 gctcacgtca cagcgcgaca tcgtcgagcc atcgcgcacg aggatccgat attcgccggg
    16681 cgccaccgcg acgcggaccg cagttccctt tgctttgtac gtctcggcga cgaccgtgtg
    16741 tgcgcgcttg tcctcgatga gcgtcttgcc ctcgagcgca gcgggcagct cgatgtgggc
    16801 ggtcgcagct ttgggatacg agagcggcac ctcgccgtgg cccttgagat cgacctcgag
    16861 cgagacgtgc tggccgccga ccgcggtctc cgccgttgcg aggagcgtct ggtgatacgc
    16921 gtagcgatac gcctcgtcga cggtgacttg gccatcgcca ttgttgtcac cggcgccgcg
    16981 caggccgacg agcaggtgat gcgtgaagta gctcgacttc agctgctcgc tctcttgcga
    17041 cagctcgctg cccgacgatg atgcgagcac cgcgacgcct gttgcatcga gttgctggcg
    17101 cgagttgaac gagaagtctg cagcgggagc cgcgcctttg acgcgcgaga aggcgccgct
    17161 ctgacacgcg tcgaggacga cgaccgtgag cttggatggc agctcgaaca gcttctgccg
    17221 cagctcgggc agcggcagct cttccttgcc gaggttgacg gcgctcgcct tcgcgtgacc
    17281 gctgtaatag aagagcacgc gcgtggggcg gccggccttt tcgtcggcgg cgagcttctc
    17341 ggcgagcttg ttgatccgag cgcgcagctc gtcgggcgtc gggtggacga cgatgtcgac
    17401 ctgatcgacc tgatagccgc cgagctcggt caggagcttg ccaacgcgac gggcgtcgtc
    17461 ttcggcatag cgcaagtcgg tctggccagg gccgccggca ttactgccaa cgacgatcgc
    17521 gtaggtcgca atgccgtcat ccgccgacgc gacggcgccg aaggcgaggg tagcgagcaa
    17581 gaagcaaacg agtctcatgg ggtcctgtgc ctatgacacg ccacttcgaa cctttgggaa
    17641 ctaggaatcg tcgtcgccac cgtctgccga agacttcgag acgtgaatcg cccgtttgat
    17701 cgcggatttg ccgaacttcg ccgccacctt gtcgatcgtg tcgccgagcc gttcgccttt
    17761 ttgccgggtc gcttcgtcga acaggagctg gcgtggcgcg tcgcgcggct cgaggctcga
    17821 tgcagcgatc ccgcagagcc gaacgcgtga gcccttcttg tcctcgatcg ggaccttcgc
    17881 gaggagctcg atcgcggtgc gcgctagaag gccgccatca gacgtcgcag catcgagcgt
    17941 agtgcgtcgc gtgatctgac gaaagtcgtc gtacttgatg atgagcacga ccgcgcgcgc
    18001 acgcaggtgg ctgtcgcgca ggcgcgcggc gacgcgatcg gcttggtcga gcagcagaac
    18061 cgagagctcg cctttgtcgt cgacatcgtc gtcgaacgtc tcttgatggc cgatcgatac
    18121 ctgctcgtgc tccgagatga cgggccgatg atcttcgccg cgtgcgagcg cagccagatg
    18181 atggcccgtg accgcgccca gccgcgcgac gagcgctgct tcggggtagc gcgcgacatc
    18241 gccgatcgtc gagagaccca tgctcgcgag caccgtgcgc gttgcttcgc cgacgcccca
    18301 cagacgcgtc accggcaggc cgtggagaaa cggcagcaga ttctccggcg tgatcacgcg
    18361 caggccatct ggcttgtcga tgtcgctcgc gatctttgca gcgagcttga tcggcgcgac
    18421 gccgacggat gccacgaggg acagctccgc gcgcacgcgg cgcttgatcg tctcgccgat
    18481 cgtcttgccg tcgccgagca ggcgctcgct tccggtcacg tcgaggaacg cttcgtcgag
    18541 cgagaggccc tcgacctcgg gcgagtagtc gccaaggatc ttgaagaaca gctcgctcgc
    18601 ttcggcgtag cgctccatcc gatggcgcac gacgatcgcg tgtgggcagc gtcgcatcgc
    18661 ttccgccatc ggcatcgccg accgaatgcc gaacttgcgc gcttcgtacg agcatgacgc
    18721 gacgacgccg cgcctgaccg tgccgccgac gagcacgggc ttgccgcgca gctttggatc
    18781 gtcccgctgc tcgaccgccg cgtagaacgc gtcgaggtcg acgtgcagga tcgtacgcac
    18841 tacgacatca tgtacgcgag cttcactcgc gtgacgagaa tcaggatcgc aacgagcgcc
    18901 gcgatcagga cgttccacgc ccacgcaggc actggcttca tctcaccgcg actgaagcgg
    18961 ccaacgcgcg cgcgcacgat cccgtgcacc gacaggatgc cgagcacgac gacggcgagc
    19021 ttgatgtgca gccagccgta gtgcttgtcg ctgaagagct tgttgccgat cgccatcgcg
    19081 aggccgcaac cgatcgcgag cgccgccgcg agatccatcg tcaacgcgag cgcgcgctcc
    19141 atgagaacga gcttttcgtg gatggccgga ggcgtctgcg tgtgaatgcg cagaatccag
    19201 taaacggtca tcagcccgcc gatccagaga aacaccccga tcaaatgggc agctaatagc
    19261 cacgcggtaa cctgggccga catgcgagga aggtaccacc tggcccgaca cgcgcggggc
    19321 gcctcgcggg tcggagtaaa tccgccccgc tccacgatcg ggcggatccc ccggtccggg
    19381 gtatgatcgc acggcccact tggcgcgcgt cctcaagatc tttttccacg acgcttgctt
    19441 cgacggcacc acttccgccg cgttgttctc ggcgttctat cgcgatgcga tcgcgaagga
    19501 tgtccagatc cagccgatcg ggatgatcca ccgcgacggc aaccccttcg agggaattgc
    19561 cctcgacgcg gatgatcacg cgtgcgtcga ctttcggttt tgcggcgatc gccggatgaa
    19621 gtggtggttc gatcaccatc cgacggcgtt ccagcccgag tcactgcgcg agatcttcga
    19681 tcgcgagcac ctgccaacgt ggttcttcga tccgagctcg ccgtcatgcg ctggcctcat
    19741 cgcgcgcacg ctcgaggaga agtacaagtg gaccccgccg ccgcacctcg tcgatgcggt
    19801 gagctgggcc gatacgatcg atgccgcgca gttcgcatcc gcagaagccg cgatcgcgct
    19861 ccggcatccg gcgcagcggc tcgccgcgtg gctcgcgcac gggcgctcga tgcacgacac
    19921 cgcgaactac gtcgagtggc tgtcgcgtgc gtcgctgtcc gaagtcgcgc agaggccgga
    19981 gcttgcaccg cagctcgcgg cggtagaagc cgaacgcacg aaggagctcg acgacgttcg
    20041 caagctcggc gtttggcacg acgacgttgt cgtcttcgat cgcttcgacg atgtcggcgc
    20101 gcgcagcccc ggcttcctcg gctacctgct gttccccgag tgtctgtacg cgatctcggg
    20161 gacgcgcacg ccgtcgagca tcaagatctc cgtcggcgtg aacccgtgga cgaacaagac
    20221 gcgccgtcac gacatcggcg agctctgcca gcgtcacggc ggcggcggcc acgcggtcgt
    20281 cggtgggatc acgctgcgcg gcgacgagct ttcgcgcgcg cgagacacgc tgaagctgct
    20341 cgttcgcgag ctgacgacgt aacggtcacc agcgtccgct gagcgacacg acgcctccgc
    20401 ccgatgcggg ctgcacctcg acgtgcgacg gcgtgtcgaa cgcgcggtac cagagatacg
    20461 cgctgaggcc tgcgcctgcg acggacacgg caacgccggt gacgaacacc gcattccacg
    20521 tgtggatctt cgaaacgtct gcgtcgtagt agctgttgtt gagcgcgagc ttcgcgcgat
    20581 cagacgcgtc gaacgccttc ccgcgagcga cgatggcgac ggcgagggca gccgcggtcc
    20641 ctgcggtcgc gacgatcggc gccaccagcg agcgcttcgg aggctggacg atcgccggcg
    20701 gtggcgcgac gacgatcgca gagccgctgc ccgcgcccgc gccgctgcct tgttcagcgc
    20761 cggagcccac gggtggtggc tcgacgacgg cgggctgttt cgcgagcacg atctgcagcg
    20821 tcatgcggtc gtgccccgtc gcttcgatcg tttgcgcgcg ctcgacgtag ccttccgcgg
    20881 ccgccttcag ctgatgcgtg cctttcggta gccacacgac gcgcgctccg acgaagctct
    20941 cgtcggcgcc gaacgaatcg atcgtcacgg acgcgccgcc gggctcgacg acgatgtcga
    21001 cgggcgtgaa gtcaccggcg cgcagcgtcg tctcgacgga gcccaggacc gcgcgcacgt
    21061 tgtcgacgaa cttcgcgtcg agcgcgctgc ctcgttcgag acatcggctg aggaacatct
    21121 gcgcgcgcgg caggtccttc gctttgaagt acgcgacccc gacgttgcag atgaggtccg
    21181 ggcgagggtc cgcggcgtag gcggctttga acttcgcggc ggccgtgacg aagtcgttct
    21241 gcttgaccgc cgcttcggcc tcacccttga gcacgtcggc ttgttgcggt gtggcatgcg
    21301 cgatcgccgc gcttcccagt accaacagga ccgacgtcca cctcacatcg cgagcgtaac
    21361 agatatactt cgacctcatg aggtggccgg tggtcctgag cgtgctcggc gcgtgtgcac
    21421 cggttccgaa tccgccgagc ttcgcgctcg acgtcgcgca ggtcgacaac aacggcgagt
    21481 ccatcaacct tcgattcggt gagctcggca tgcagctcgc gccgatgacg agcatccagc
    21541 tgccgctcgt tctgacgccc gatggtgggg cgacgaacct gctcgaaccg ccgcccgcta
    21601 cgaatccgtg cggcgaaggc tatgccggga tcaacctcaa cccgatcgct cgcgcgagcg
    21661 cgtcgacgat gatcggtggg tcgacggtga ggcagctgct ctcgggcccg gccgtcgccc
    21721 gtgttgcggt cgagtacacg gtgccgtaca tgtgcaacgg caaccgcgag ctcaagggaa
    21781 agacgacgtt cacgctgttg ccatcgggac gcatcgagcg gctcgacgag aacgtcgtgg
    21841 cgtcgatgac gaacgttccc gataccgcga gctgcggctg tgcgaccgcg cccgacaacg
    21901 cgatctatta ctcgtcgttc tggtcgttca agcccggcac ggacaagacg ccgaccgata
    21961 cgacgcgtgg cagtgagacc gggccgggct gcacgatcga ggcgtccggc gacacgatcg
    22021 ccgtcgacta ccacaacagc gagcagcgcg gactcaacgg aacgcacctc gtctacttca
    22081 cgacgtctgt gccgccgctc acgaagatcg agccacaaac ggcgcagtcg tcgctcgtca
    22141 tcggcaagaa cctccggtgt gcggatgcac tcgcgcgtct cgaggatccg ccgatcatgg
    22201 tcgacgacaa gatggtgaca gcgaaacagg gcatctacgt cgacacggtc aatcatgcgt
    22261 cgcgcgcgga gatcacgacg cagttcccgg tcctgcacgg ctttgcgctc gagattccgc
    22321 tcaacggcgc aacgcacgca cgcgtcctga agaacggcga cccatttccg tttgccgtcg
    22381 agaccgatgg cgacaatccc gtgttctggt tccccgaccc gctcgtcctc ggcgacacga
    22441 tcgtcatcga tcagttcagc gagtgatcac caacggccgg tcagtacgaa cgtcgcgcca
    22501 tcgccgttgc tcgtgacctc gaggtgcgac gtctccgtgc gcgtgacgcg ataccagaga
    22561 tagccgctca cggccgcacc ggcgacggcg agccctgcaa agccccacgc gatgttttcg
    22621 cgcaggtgaa cgccatcggc gatgtcctgc cactcttcca tcgactgcgc gtcgggtgcc
    22681 gtgagcgcct tcgctttcgc gagcgagtag cacgcgagcg cggctccgac ggcgacgaat
    22741 gacgcggtcg tcacgacgat tggaacgaca cggttcttgc gaatcggatt gatcaggatc
    22801 gccggtggcg gcgcttcgac cggcgagccc tcgctggcga tcgcgctgcc actgccacta
    22861 ccggccggtg gtttctcgac gaccggcgca cgctcgagcg tcacgcgcgc ggcaaccggc
    22921 gttcgggtct ccgcgttgac ctcgacgatc gtgtcgacgt gtccttcggc gtgaacggtc
    22981 agcttgtact cgccgaacgg aaaccagacg cgcctcgatc cgacgaatgc ctcgtcatag
    23041 accgcgttcg ccgtaaacga tgcggtcgtt ggctggacga gcacgtcgac cggcgtgtag
    23101 tcgccgcgcg taagcatgtc gttgacggcg tcgagcacct tcttgacgct gtcgatgaac
    23161 gggcgatcga gcgacgtccc gatctcgagg cagtgcgcca ggtagcgctg cgctcgaggg
    23221 aggtcacggg ccttgtaata cgccacacca acgttgcaga ggagatccgg gcgcggcgcg
    23281 gcagcgaatg cttcgcgata ctttgcggca gcgccgggga agtcgttagc ggccgctttg
    23341 cgctgggctt cggcgacggc gtcgtcggcg ggatccgcgt caccgcgcga cacgcacata
    23401 gcgacgagca tcacagcagc ggcaccgcgc acggatcgag cctacccgga tatactcacg
    23461 cggtgaagcg gcggttggtc tggggcgcgc tattcggagc ggtgctcggt gcgtgcatct
    23521 cgatcccgcc gtacgcgcag aaggatgctg gcggtgatgg cgccccaacc gacggctcgg
    23581 gcagcggcac gccggcgacg gtcacgtaca tggacgatgg ctcgggcggc ggcgtcgtcg
    23641 tcggcccgtt cttcaacatg catttcgcgg gcggaacgaa accgcactac cccgacgcga
    23701 tctcgatcga caacctgcca gttctcggac attcgacgtc ggcggcgtgc gctgccgagg
    23761 acgaacatgg cgtcgccttc tatccgtctg gtcgcgtgtc gccaaacggt ccggccaacg
    23821 tcagcgaagg gacgatgctc gatgtcgtgt ggcgcgggcc cgcgatcgtg aaggtcatcg
    23881 ccgagacgaa cgtccggatc gatggctccg actcgagcgc caacgcggtg acgcgccatc
    23941 caaccatcac gtcgcgctac acgattcatc cagacggcaa agtgttccgc tacgacacga
    24001 tcgaaggcgg gaccggcgcg gcgatcacac cgacgagcat gagctgtttc ggtggaacgc
    24061 cggggcagtt catcgcgacg tcgttctcga cgtggcagcg cgaggccggc tcgcacctgt
    24121 acgtgcacag cgggacgacg gcgatcgata cgccggtgcc tgaattcaac atgggtgcga
    24181 ccgacgtcca gaatcggacg agcgcgtgtc tcgactacgg ccagcgccag gtcgccttcg
    24241 gttggtacga cctcaattcg cgcattcgcg cgccgaccgc cgacacgctc gcgttcgtct
    24301 acgactatgc cttgaccgcc gagtccgaga tgggcgccgc cgattactgg acgcgttcga
    24361 tgttcatgct cgggcgcggg aacagctgca cgaccgatct tccgggcatc tacagtcagg
    24421 tccgcgcctg ggagggcagg cgggagatgc tgaacatcaa cgggctcggc gtcgagatcg
    24481 cgaatgacgg catctacggt ggcgaggccg atccgggcag tacgcccggc atctggtatg
    24541 gcggcgatcc agtcacgctg acaggcacga tctcgattcc gtttgcggtg tggctgatca
    24601 tgtcgcccgc ggcggatgac atcgtcgtcg agaaagccga cgcgcagggc cccttctatc
    24661 agaagcagcg cgacgcctcg ggaacgaact gggtgatctg gtttcgcgat ccgctctctt
    24721 ctggtcagtc gattacggtc aggcgcgctc cgtgaccaag ccgatggatc cgacgatgcc
    24781 gagcgcgagc gatcttgcgg agacgcaggt ccacgccgag gaggcgtact gctccgcatg
    24841 caatcagtcg tatccggcgg gcacgttgac gtgtcccaaa gacggcgcgc gtctgatcaa
    24901 gctcgcggcc gagacggaca cgctgatcgg tcgcgtcctc gaccagcgct acgagatccg
    24961 ttcgcagctc ggtaagggcg gcatgggctc ggtctatcgc gggtggcagc tctcggtgga
    25021 tcgagaggtc gcgatcaaag tcattcaccc gaagctgtcg aatgaccgct cggcggtgaa
    25081 gcggttcctg cgcgaggcgc ggctcgcgag ccggctcagc cagccgaaca tcgtcaacgt
    25141 ctacgacttt ggtcagtcgg aaggcgtgct gtatctcgtg atggagctgt tgcgcggtca
    25201 cacgctcgcg agcgagctcg gcaagggcag gcggctcaat ccgaagcgca cggtgacaat
    25261 cgccagccag ctgtgcgacg cactcgaagc tgcacacgcg cagggcatcg ttcaccgcga
    25321 tctcaagccc agcaacatcg tcatcctcga cgatccgccg ggccgcgatc tgatcaaggt
    25381 cctcgacttc ggtctcgcga agtcgctcaa gcaagactcg ggctcgctcg tcacgaacac
    25441 cgatgcgctc ctcggcacgc cgctctacat ggctcccgag cagatcgacg gtcgcgagag
    25501 cgatcagcgc gccgatctgt actcgctcgg ctgcatgctc tacgagatgc tgtcgggccg
    25561 gccgccgttc gtcgacaacg cggtctcggc ggtgctcgcg cgtcacatgc acgatacgca
    25621 tgtcgagctg ccgcctcacg tgccggttcg gttgcggtcg atgatcgacc agctgctcgt
    25681 gaagaagccc gaggatcggc tgcagtcggc gggcgacgtg cgcaagattc tcgaggacgt
    25741 tcgcgattcg gcgccggtgg tagccatccc gatgtcgccg gggatgtcga tggatatccc
    25801 cgatacgatt ccggacgtct cgcccgcact cgccgaaacg ttcgcggcga cgcccgtgcc
    25861 ggagagccag gtgctcgaca gcgcgccgaa gagcaggaag tcgacgccgc ggatcgatac
    25921 gtcgacggat caggtgaagc ggccgcaacc acgtcgcgtt gcgatcttca tggcgatcgc
    25981 gatcgtgccg attgctgtcg tcgcgctgct ggtggtcaag tcgatgaaca accgtgcgag
    26041 caccggcgag accggcggcg cgggtaacgg gtccgtcggg agcagcagcg gcagcggcgc
    26101 tccgcagaga ctcgccgtgc cgcaagacgc ggccctcgcg catccgcccc ccgtcgatgc
    26161 aacggagatc gacgcgcgag gtgcgtcgac gacgcccgac gcgggacggc atcgcccgcg
    26221 cgtagtcccg ccgacctcga aggttgatgc cggcgcgacc gagccgaaca ttgatttcta
    26281 cgaagagaag cccaagaact agcggcgctt cttcttcggc gcgcgcttgg acgcgggctt
    26341 cttcacggct ggcgcattgc gcgcttccat catctcgatg cgctcgcgca gcgtgccgac
    26401 ctgattgcgg agctcgccga cttcgtcgcg caagcgtcgg acgtcctcgg cgagcggcgc
    26461 gccttcaccg agatcgcgga caatctcttt gcctcggcgg cgcaggcggc catcgagctc
    26521 gcgatcgatc atccggcgca gcgcgggaat cgctcccgga tcgccgatga cgaacagtgc
    26581 ttccatcgcg gcggcctgca cgcggaagtc tttgtcgacg agcaggagct cgatccgctc
    26641 gcgcacatcg cgcgcttcgc gatcgcggcg gccgcgcatg agaatcgcga gtgcacctgc
    26701 agcggcgcgg cgaccctgcg tcggacgtcc ccacttgagc ccgtcgacga gcaggccgag
    26761 cgcactgtca tcgcgtgcct ccgcgagacc gcgatacgca tgctggcgga tcacgtcggt
    26821 gaacgactcg cgcgtcgccg catgacggag cagctcgccg gcgcggccgg tgcgcgtgcg
    26881 cccgagcgcg aggcacgctt cggcctcgac gaagtagctc gcgtcgccct tgtcgacaat
    26941 cgcggcgagc gcggcggctg cagtgccgtc gtgtacgaag tcgccgagcg cacgagcgat
    27001 cgagcggcgc gcgcgagggt tgacctcggc cttgagccgc ttgatcagac gatcgcgcgc
    27061 ggccggtgtg cgcagcttgc cgagcgagag cgccgccgtt ccgcggacgg cccagaacgc
    27121 gtccttctcg agcgcggtca ccagcgctgc ctcggccgcc ggtccgccgc gcttcgccag
    27181 ctcgatcgct gcagcaccgc gatcgatcgc gagcgtcgcg ccggcaagct cggcgatcca
    27241 caccggttcg ggcttgt
//
LOCUS       DATLBZ010000112         3719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6761375, whole genome shotgun sequence.
ACCESSION   DATLBZ010000112 DATLBZ010000000
VERSION     DATLBZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6761375"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..880)
                     /locus_tag="VMZ53_06005"
     CDS             complement(35..880)
                     /locus_tag="VMZ53_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS28039.1"
                     /translation="MSNPALASEIIGLTPPDQRDERWHIRVRQLTKSFGPNLVLDKVD
                     LDLERSKVNVILGASGAGKSVLIKHFMCLLRPDSGHLYVDGTDVFGLDTKKLSEFRRR
                     FGLVFQFSALFDSLTVEENCMFPLKEHTKKTVAEMRDIAGDRLAALGLAGTQKKYPGE
                     LSGGMRKRVGLARALVLEPEILMYDEPTTGLDPLATRNVDEMILEAGERYKVTSVVIS
                     HDMASVFRIADRIAILHNKRILEAGPAEVIAASRDPYVYEFLRASGVSAIGKLAGSAS
                     NGGQL"
     gene            complement(887..1804)
                     /locus_tag="VMZ53_06010"
     CDS             complement(887..1804)
                     /locus_tag="VMZ53_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS28040.1"
                     /translation="MTGDEETTNVAPVAAPPAPPAAQPPAVVPAEPKITVGKAVLTGV
                     KDAGAPVYNFVDVIGAHIIMVGRAFAWLPRRPFRARNYFEAMEYIGFGSLPIVLLVGV
                     FTGMVTSLQSVNAFRIFGFESFAGGATGKGLAVELGPVLTSLMLSGRVAAGIATELGT
                     MRITEQIDALESMAVNPHQYLILPRVIAAMIVTPILTLIFFVVGMGGAYFVAVIVEQV
                     PQGPWIANLREIVEARDVLQGCLKAIFFGFMVALIGCYQGYHAQGGGRGVGIGTTRAV
                     VIASVSTLVLDYFLTDILLHVMPSSRGQW"
     gene            complement(1801..2943)
                     /gene="alr"
                     /locus_tag="VMZ53_06015"
     CDS             complement(1801..2943)
                     /gene="alr"
                     /locus_tag="VMZ53_06015"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HUS28041.1"
                     /translation="MIRRTRVEIDLQAIVGNAEAVRKVTGTPLYAVVKADAYGHGAVA
                     VARALADAKAAQGFCVSLVEEGVALRDAGITAPILVMGPSQVGGEEEMVEAKLVPVIS
                     SDEELAALAHVANGRKEPVEAHLKVDTGMGRLGVAVDRAAELAATAGKNGIRVVGLMT
                     HFANADTDEPDDSSSLTRQQLARFAEVDRAVVAAGAPVRMRHAANSSGALVFPEARFD
                     LVRCGIALYGNGRWAGPAERGVAPHQVSQAMRLVTAVAQIRRVPAGQSVGYGATWRAP
                     RDSRVAVLPCGYADGLPRRASGHAQTAIRGKRVPLVGRISMDIALADVTDIPDVAVGD
                     AAVLLGRASNGATITTAEYGSWSELSEYEVTCGMSKRVPRTYVEAA"
     gene            complement(3161..>3719)
                     /locus_tag="VMZ53_06020"
     CDS             complement(3161..>3719)
                     /locus_tag="VMZ53_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HUS28042.1"
                     /translation="PGDAMFVIMSGRVKVVIFGESGREVTLSLLRAGDSFGEMSLFDQ
                     APRSAHCLAIEPTTLLVLSRDDLMKHMQAHPRTAINLVSEMARRLRRADETIAQLALC
                     DVNERLIHRLVALGREEGASGPDGLVGRRRPTQQELANMIGSCRETISRAFNQLARDG
                     LIIPRGRSLVVTPALIERAEKKKAA"
BASE COUNT          650 a         1248 c         1234 g          587 t
ORIGIN      
        1 aggatgccga cgcgcacgcc tgcagagagg cctctcaaag ttggcctccg ttcgacgcac
       61 ttccggccag cttgccgatc gcgctcacgc ccgaggcgcg caggaactcg tagacgtacg
      121 gatcgcggct cgcggcgatc acttcggcgg gccccgcttc gaggatccgc ttgttgtgca
      181 gaatggcgat gcggtccgcg atccggaaca cgctcgccat gtcatgcgag atgaccaccg
      241 acgtaacctt ataacgctcg cccgcctcga ggatcatttc gtcgacgttg cgggtcgcta
      301 ggggatcgag gcccgtcgtc ggctcgtcgt acatcaggat ctcgggctcg aggacgaggg
      361 cccgggcgag gccgacgcgc ttgcgcatac cgcctgacag ctcacctgga tacttcttct
      421 gcgtacctgc gaggccgagc gccgccagcc ggtccccggc gatgtcgcgc atttcggcga
      481 cggtcttctt cgtgtgttcc ttcagcggga acatgcagtt ctcctcgacg gtgagcgagt
      541 cgaacagcgc gctgaactgg aacacgaggc cgaacctgcg acggaactcg gacagcttct
      601 tggtgtcgag gccgaacacg tcggtgccgt cgacgtagag gtgaccactg tcggggcgca
      661 gcaggcacat gaagtgcttg atgagcacgc tcttgccggc gcccgacgca ccgaggatga
      721 cgttgacctt gctgcgctcg agatcgagat cgaccttgtc gaggacgagg ttcgggccga
      781 acgacttcgt cagctggcga acgcgaatat gccaacgttc gtcgcgctga tcgggaggcg
      841 tgagcccgat gatctccgat gcgagcgcgg gattactcaa ggcgcctcac cactgacctc
      901 ggctcgacgg catcacgtgc agcaggatgt ccgtgaggaa gtagtcgagc acgagcgtcg
      961 agaccgacgc gatgacgacc gcgcgcgtgg tgccgatgcc gacaccgcga ccaccgcctt
     1021 gcgcgtgata gccctggtag cagccgatga gcgcgaccat gaagccgaag aagatcgcct
     1081 tgaggcagcc ttgcagcacg tcgcgcgcct cgacgatttc acgcaggttc gcgatccacg
     1141 gtccctgcgg cacttgctcg acgatgacgg cgacgaagta cgcgccgccc atgccaacga
     1201 cgaagaagat cagcgtcagg atcggcgtca cgatcatcgc cgcgatcacg cggggcagga
     1261 tcaggtactg gtgtgggttc acggccatcg actcgagcgc atcgatctgc tcggtgatgc
     1321 gcatcgtgcc gagctccgtc gcgatgccag cagccacgcg acccgacagc atcagcgagg
     1381 tcaacacagg tccgagctcg accgcgaggc ccttacccgt cgcgccaccg gcgaacgact
     1441 cgaagccgaa gatgcggaat gcgttgacgg actgcagcga cgtcaccatg cctgtgaaca
     1501 cgccgacgag cagcacgatc ggcaagctgc caaagccgat gtactccatc gcctcgaagt
     1561 agttgcgcgc gcgaaacggc cggcgaggca gccacgcgaa ggcgcgaccg accatgatga
     1621 tgtgcgcgcc gatgacgtcg acgaagttgt aaaccggcgc gcccgcgtct ttgacgccgg
     1681 tcaacacggc cttcccgacg gtgatcttcg gctccgccgg gacgacagcg ggcggctgcg
     1741 cagcgggtgg cgccggcggc gcagcgacgg gcgcgacgtt cgtggtttcc tcgtcgccgg
     1801 tcacgcagcc tccacgtagg tgcgaggtac gcgcttgctc atcccgcagg tcacttcgta
     1861 ttcggagagc tcggaccagc tgccgtattc agcggtcgtg atcgtcgcgc cgttgctcgc
     1921 gcgtcctagc aacaccgcgg catcaccgac ggcgacgtct ggaatatccg tgacgtcggc
     1981 gagcgcgatg tccatcgaga tccgcccgac gagcgggacg cgcttgccgc gaatcgccgt
     2041 ctgcgcatgg ccactcgcgc gccgcggaag cccgtccgcg tagccgcatg gcagcaccgc
     2101 aacgcggctg tcgcggggcg cgcgccacgt ggcgccgtag ccgaccgact ggccggcagg
     2161 gacccggcgg atctgcgcga ccgcggtgac gaggcgcatt gcctggctca cctgatgcgg
     2221 cgcaacgccg cgttctgcgg gaccggccca gcggccattg ccgtagagcg cgataccgca
     2281 gcgaacgaga tcgaagcgcg cctcgggaaa cacgagagcc ccgctcgagt ttgctgcatg
     2341 ccgcatgcgc acgggcgccc cggcggcaac gaccgcgcgg tcgacctcgg caaagcgggc
     2401 gagctgctgg cgcgtcagcg aggacgagtc gtccggctcg tcggtatcgg cgtttgcgaa
     2461 gtgggtcatc aggccgacga cgcggatgcc atttttgcct gcggttgcag caagctcggc
     2521 tgcgcgatcg acggccacgc cgagccggcc catccccgtg tccaccttga ggtgggcttc
     2581 gacgggctcc ttgcgaccgt ttgcaacgtg ggcgagcgct gccagctctt cgtcgctcga
     2641 gatgaccggc acgagcttgg cctcgaccat ctcctcctca ccgccgacct ggctcgggcc
     2701 catgacgagg atcggcgcgg tgatgccggc gtcgcgcagt gctacgccct cctcgaccag
     2761 gctcacgcaa aacccttgtg cggccttcgc atcggcgagc gcgcgcgcca ccgccacggc
     2821 gccgtggcca tacgcgtccg ctttgacgac cgcatagagc ggcgtcccgg tgaccttgcg
     2881 caccgcttcc gcgttgccga cgatcgcctg caagtcgatc tcgacccgcg tacgccggat
     2941 catcgtcccc tcaaaggaca taagtgatac gaggatttgg cgccccgtcc gaggacaagt
     3001 caaggttacg ggatccgagt cacggcatgc cgatcgcacg tgtggcgcat gcgccacgcc
     3061 gcatgacaac gcggtcgcga ccgccgaaaa cgaaaaacgc cgcctttgcg gggcggcgct
     3121 tctagctagc gtcgacggag cagtgacgct gctagcgacc tcaggcggcc ttcttcttct
     3181 cggcgcgctc gatcagggcg ggcgtcacga cgagcgagcg gccgcgcggg atgatgagac
     3241 cgtcgcgggc gagctggttg aacgcgcggg aaatcgtctc gcggcagctg ccgatcatgt
     3301 tggcgagctc ttgctgcgtc ggccggcggc gaccgacgag gccgtccgga cccgaggcac
     3361 cctcttcgcg gccgagcgcg acgaggcgat ggatcaggcg ctcgttgacg tcacacagcg
     3421 cgagctgcgc aatcgtctcg tcggcgcggc gtaggcggcg agccatctcc gagacgaggt
     3481 tgatggcagt acgcgggtgc gcctgcatgt gcttcatgag gtcgtcgcgc gagagcacga
     3541 gcaacgtggt cggctcgatc gccaggcagt gagccgaacg cggcgcttga tcgaacagcg
     3601 acatctcgcc gaacgaatca cccgcacgca acagcgacag cgtcacttcg cgaccgctct
     3661 cgccgaagat caccactttc acccgcccag acatgatgac gaacatcgca tcacccggc
//
LOCUS       DATLBZ010000113        10550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2344245, whole genome shotgun sequence.
ACCESSION   DATLBZ010000113 DATLBZ010000000
VERSION     DATLBZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2344245"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..735)
                     /locus_tag="VMZ53_06025"
     CDS             complement(43..735)
                     /locus_tag="VMZ53_06025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS28043.1"
                     /translation="MRRLLLLLAACGSSPHAASSVSEPMHQHHHHEHDDGHGPLGHRF
                     EHADEWAPKFDDPARDAWQQPDRVVAALELTPGMIVADIGAGTGYFEARLSKAVGDGG
                     KVIAVDIEADMVRYLRERATREGTANVEARQATPEGPPLAAASIDRILIVDTWHHIPD
                     REAYAKKLAVALKPGGAVFVVDFTQETDKGPPREHRIPAEQVKRELEAGGLHASVVDV
                     ALPDQFVIKGAR"
     gene            complement(751..2709)
                     /locus_tag="VMZ53_06030"
     CDS             complement(751..2709)
                     /locus_tag="VMZ53_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HUS28044.1"
                     /translation="MVMALALGALVGAACKKSNDTSAKDTPKTQESKTTSATEKATTQ
                     GGTQTDIGVQAGGIAHSDSEGAAAWLAAVSGTVEVRRVGETQWTAAKQDMKLYEGDMI
                     RTADQGSATIAMADQSTVEMAETSSMAIASRDASADPASGAAMLGGMARFTVTPRAPG
                     EGAFRVYTSSGVILTKGTTYGVGVSASGEARVGVESGTVDVIGLAQMDATPVAVSTTN
                     QVVIAADGTVGAATAWPSDDWGTWRDETDAKMQLTAAMDAHGAAMTDLNKQLMEGYAQ
                     LQTNADAAATFEATAATAAEKQDTAAYTAALPEGSATIDASFSLAGRIEALTWAYCSH
                     AELATELWMRHPKDVEASWTVMAPSVDAAVLWPKRWEVTATAYLEPLRMQYYVHHPRG
                     RMHAKLVGVTVPEWYASVQAPAVDPATVRAKAKLAFWMTPDVKWQASSPRPVWITAPD
                     ASWHASVKATPATARGKVAWYVRPPTMKATALVGANVQSNWESKLKMQPAMPMADLHA
                     MWKVPVGTKIKIAAPDMKAAANARASWKADAKWNADVAGQAQGKVHAPNVATDVKGKM
                     EVKAPDVHVQVPDVNVKGKLDVAAGAGAKATADAKAGAKAAANAGADAAGKVDGAVKA
                     SVKAPEVKVKAPSVKVEGKAKGSFKLGN"
     gene            complement(2822..4252)
                     /locus_tag="VMZ53_06035"
     CDS             complement(2822..4252)
                     /locus_tag="VMZ53_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28045.1"
                     /translation="MAVVVAACAEGGDSIVDEAEVSADSKVDAASELTVRAGDTTLWV
                     NKTVARRGDLYVLRGRTSRNLTDGRGFIFDDVYGDFAAKTARTFELTWPVSTARGLVD
                     GVEQFIGMTFVHSSSRPDSLTARVVVRPRLAAFTGSTKIYLVSELTPVVYGGTVVYRV
                     QGHTYGANTGVRAVVDGVELSDVRRIDNEKFVVDLQPEQAFALTAGGDLQIVADFATG
                     GVEKRATLGLGIKKLGMTSGDVEAVWPPMQCTSSVRSCLTGLADGSLDLGGCGVATEV
                     NACAGQVGVFVDDVSFQATLHTADVRLSTAAAKADAAALVGSDKANEWLFGAKETVEG
                     ELQQQFGRWYLSANARTQMLSAALERGLDHAYARPLDVVEAHAPVPGNAAAMRQVAAD
                     AVLAELDSMDFVHTEFARTLESLTHEFRTQHIASLKEFRESIDPEPYPGHPEWDVYVG
                     QWLGLHTEITVVRTTGDVVQRLVEID"
     gene            4329..4586
                     /locus_tag="VMZ53_06040"
     CDS             4329..4586
                     /locus_tag="VMZ53_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HUS28046.1"
                     /translation="MSDHPTDFQGSIPDAITTSIQGKLPDAKVEVSGGGGHWSIVVVS
                     AAFEGKSILEKQRMVLSSIKHLLDGAAPPVHAVDSLVTKTP"
     gene            complement(4583..5890)
                     /locus_tag="VMZ53_06045"
     CDS             complement(4583..5890)
                     /locus_tag="VMZ53_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28047.1"
                     /translation="MVVLAACGPQNNATGDDDDVGGNGQVDAATGSGSGSGSGSGSGA
                     NGPGSDCSCDADCADDAGHDGVCIYGVCMTKASTNCSAAGSQAECPTGSRCWNLDGHT
                     GSLCWPDCAAHMCAGACASDGSCEPTAQTDCDPTCGEACSCTATSCGTGQSCVSGECV
                     PDVGAGPGAGPGPTCSSLPTRDCTTGCSTLTPFSPRTTTAYDDYAINGETSTNQYRSY
                     PRKDLNMLVAWATAKTACKTAAWTAGNGGPLGLGDMSEANGAIPGTSIGSPGHPAGTH
                     VDGYDIDLGYYQIGTADNRLRPICNTVVNGQDQQHCVADPVYLDVWRHAFFLGSVFES
                     SRTRVIGVDGKAGPKILSAMNQLCNSGWLSAAACSNAVLAYETTDGGAGWYYFHHHHS
                     HISLKHTALVNDKSTTLECDSPLGCNPTVRSIAKRPAGLHRLR"
     gene            complement(5945..7303)
                     /locus_tag="VMZ53_06050"
     CDS             complement(5945..7303)
                     /locus_tag="VMZ53_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28048.1"
                     /translation="MRMRLTLLVLGFAGSAYAAPSAEDELAVSQLELGRRRDVTDELP
                     ILVLGMSRDIPEEAKLDPYVRSAAFAQLAGGGEGGGGAAAGAAMAIGGIGCDIAHGSA
                     QGRFRPFAHDEHVTGQVDYGACLSRVAFTIAFDGTRGIGLAPSLSDRRSLWSRRYATA
                     YDQIEFGAGELYNRRNPNARHTILPMIFGHGETTQVDGSESRTIVTLDLDLAAYRYRR
                     VEGLQIDAAVLESNALKAGIDNRGGVASAFYPARVSYDDGVVFVQAAAGWGMSGGSVT
                     QSSETKVNGEVVDSWTETIDSEGLPNMTIFVGRLQGGVRGDRLTTSAAVSRSFYPTFD
                     GNIAREARVSGDVTYVAGRTRRTTLSLAPFATRTHTWTREDGSIVSFPPRDVSAGASL
                     HVGRELTKQLRVDAIGEAGVSPYARLDRERLPSGSVGGQVLVALTGKVTDFTGQLTKV
                     QH"
     gene            7423..8421
                     /locus_tag="VMZ53_06055"
     CDS             7423..8421
                     /locus_tag="VMZ53_06055"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HUS28049.1"
                     /translation="MKKLGVAIVLLVAALAGCNKKAADKAPETNAGSGATTTPQTGSA
                     GSAGMAGSAGSAAEVAWDGKTPLSLEGFQTPESVLYDADGDGYLVSNIGGQPLDLDDN
                     GFISKISPDGKITELKWIDGAKDNIKLNAPKGMAITGGLLYVADIDTVHKFDAKTGEL
                     KGDIKIDGATFLNDVAVAPDGGVYVTDSGLDKEFKGTGSDAVYMIGKDDKVKPVMKDK
                     KLAAPNGITAGDKGAVWVVTFGSGEIYEVDAKGKQGKNQNLPKGQLDGVVVLDGGEVL
                     VSSWEGSAIYRGKPGAEWKPVVENVKSPADIGYDTKRHRILIPVFMGNTVIIQPTE"
     gene            8418..9083
                     /locus_tag="VMZ53_06060"
     CDS             8418..9083
                     /locus_tag="VMZ53_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28050.1"
                     /translation="MIPWKELGRARVGKRDLVLARRGDEFALRVGGAELMNSRQHSSE
                     EALAKLGCATLGSVPHARVMIGGLGMGFTARAALDVLPSDARVTIVELVPEVVAWNRE
                     HLGDLAGRPLDDPRVTVLECDVTQVIRESAETWNAILLDVDNGPDAFTSPDNAKLYGL
                     KGLMACRKALVRGGMLGVWSVENDNAFTGHLTAAGFDVDKQRVPSRPGSSVKHVVWIA
                     RKK"
     gene            complement(9084..>10550)
                     /locus_tag="VMZ53_06065"
     CDS             complement(9084..>10550)
                     /locus_tag="VMZ53_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HUS28051.1"
                     /translation="INALSFRHMASGEAPPSGHPFLVMNSNSEVHNAGEIWASALWQV
                     YAALQKSSTDFLETRAKMARYVVAGLALTPTEATPMEARDAILSAVEGASSYDHGLML
                     DAFASRGFGSCAIAPPPASTDFTGLKESFLVAGRADVATLELDDACDHDGVLDSGETA
                     HIRIQVANRGHAALTNVSVRVTSSDRTIDVDNTPRAIGRLEPSESVELPVDVAIEAGH
                     TEAIEGTLSLEVSGDGACETVTAFPIVARLNVDDVPAASTTDSFDALESVWVPWTAAW
                     THRRSSPLNGYWHASDLELDSDTRLTSPYLHGSATEPVTLTFKHRHSLEVTDGIAYDG
                     GVIEYSLGGDEIWQDLSTLTNVPYTDVLQADTANALTGKPAFTGKNASYPAMDTITLD
                     LGTALADQTFRIRFRLGTDEATAAQGWDIDDVALTGIDGAPFPAQLPDDGLCGEEPPP
                     EEDPIFAGGGGCCDGGSAGGSGLVSLGVLALVLRRRRR"
BASE COUNT         1730 a         3521 c         3479 g         1820 t
ORIGIN      
        1 atcacgccca tcgcctcgcg accataggcc gcccctcgat agctagcgag cgcccttgat
       61 cacgaactga tccggaagcg cgacgtcgac cacgctcgcg tgcaggccac ccgcttcgag
      121 ctcgcgcttc acttgctccg ccgggatgcg gtgctcgcgt ggcggcccct tgtccgtctc
      181 ttgcgtgaaa tcgacgacga acaccgcgcc accgggcttc agcgcgaccg cgagcttctt
      241 cgcgtatgcc tcgcgatccg gaatgtgatg ccacgtatcg acgatcagga tgcgatcgat
      301 ggacgcggcg gcgagcggcg gcccctctgg cgtggcctgt cgcgcctcga cgttcgccgt
      361 accctcgcgc gtcgcgcgct cgcggaggta gcggaccatg tccgcctcga tgtcgacagc
      421 gatcaccttg ccgccatcgc cgactgcctt cgacagccgc gcttcgaagt agccggtgcc
      481 agcgccgatg tcggcgacga tcattcctgg cgtgagctcg agtgcggcga ccacgcgatc
      541 gggctgctgc cacgcatcgc gcgctggatc gtcgaacttc ggcgcccact cgtcggcgtg
      601 ctcgaagcgg tgaccgagcg ggccatggcc gtcgtcgtgc tcgtgatggt gatgctgatg
      661 catgggctcc gaaacggacg acgccgcgtg cggcgacgag ccacacgcgg cgaggagaag
      721 tagcaggcgg cgcattcgcc gcgagtctac tcagttaccg agcttgaagc tgcctttggc
      781 tttgccttcg actttgaccg acggcgcctt caccttcact tcgggcgcct tcaccgacgc
      841 cttcacggca ccgtcgactt tgcccgcagc gtcggcaccg gcattcgcgg cggccttcgc
      901 gccggccttc gcatcggccg tggccttcgc acccgcacct gcggccacgt cgagcttgcc
      961 cttcacgttg acgtcgggca cctgcacgtg gacgtcgggc gccttcactt ccatcttgcc
     1021 cttgacgtca gtcgcgacgt tcggcgcgtg gaccttgcct tgcgcttggc ccgccacgtc
     1081 tgcgttccac ttcgcgtcgg ccttccagct cgcacgcgcg ttcgcggcgg ccttcatgtc
     1141 aggcgcggcg atcttgatct tcgtgccgac cggcaccttc cacattgcgt ggaggtcggc
     1201 catcggcatc gccggctgca tcttcagctt gctctcccag ttcgactgca cgttcgcacc
     1261 gacgagggcg gtcgccttca tcgtcggagg gcgcacgtac cacgcgacct tgccgcgcgc
     1321 ggtggcgggc gtcgccttga cgcttgcgtg ccacgacgcg tcgggcgccg tgatccacac
     1381 cgggcgcggc gacgacgctt gccacttcac gtcgggcgtc atccagaacg cgagcttggc
     1441 cttcgcgcga acggtggccg gatcgacggc gggcgcctga accgacgcgt accactcggg
     1501 caccgtcacg ccgacgagct tggcgtgcat gcgaccgcgc gggtgatgca cgtagtactg
     1561 catgcgcagc ggctcgaggt atgccgtcgc ggtgacttcc cagcgcttcg gccagagcac
     1621 ggccgcgtcg acgctcggcg ccatcacggt ccagctggcc tcgacgtcct tgggatgacg
     1681 catccagagc tcggtcgcga gctccgcgtg cgagcagtac gcccacgtca gcgcttcgat
     1741 gcggccggcg agcgagaacg atgcgtcgat cgtcgcggag ccttccggca gcgcggcggt
     1801 gtacgcggca gtgtcctgct tctcggcagc ggtggctgca gtcgcttcga acgtcgcggc
     1861 ggcatccgcg ttcgtctgga gctgcgcgta gccttccatg agctgcttgt tgaggtcggt
     1921 catggcggcg ccgtgcgcgt ccatcgcagc ggtgagctgc atcttcgcgt cggtctcgtc
     1981 gcgccacgtg ccccagtcat cgctcggcca cgccgtcgcg gcaccgaccg tgccgtccgc
     2041 agcgatcacg acttgattcg tcgtcgagac ggcgacgggc gtcgcgtcca tctgcgcgag
     2101 gccgatgaca tcgacggtgc cactctcgac gccgacgcgt gcttcgcccg aggcggagac
     2161 gccaacgccg tacgtggtgc ccttcgtgag gatcacgccc gacgacgtgt agacgcggaa
     2221 cgcgccttcg cccggtgcgc gcggcgtgac ggtgaagcga gccataccgc cgagcatcgc
     2281 tgcgcccgat gcggggtctg cgctcgcgtc gcggctggcg attgccatcg agctcgtctc
     2341 ggccatctcg accgttgact gatcggccat ggcgatcgtg gcgctgccct gatctgccgt
     2401 gcggatcatg tcgccctcgt agagcttcat gtcctgcttc gcggcggtcc attgcgtttc
     2461 accaacgcga cgcacttcga ccgtgccgct gacggcagcg agccatgcag ctgcgccttc
     2521 cgaatccgag tgcgcgatgc cgccggcttg cacgccgata tcggtctgcg ttccgccctg
     2581 cgtcgtcgcc ttctccgtcg cggaagtcgt cttcgactcc tgcgtcttcg gggtgtcttt
     2641 cgcggacgta tcgttcgact tcttgcatgc cgcaccgacg agagcgccga gcgccaacgc
     2701 cataaccata gaacgtttca tcgttcctcc ttttgaggac gacgatgctg cgcgcgtaaa
     2761 attggcacca caaccctcat tcacgtgagg gcctcgttag tgtgcgcgtg cgcccgtttt
     2821 gtcagtcgat ctctacaagt ctttgaacaa cgtcaccagt tgtacggacc acggtgatct
     2881 cggtgtgtaa gccgagccac tgaccgacgt agacatccca ctctggatga cctggatacg
     2941 gctctggatc gatcgactcg cgaaactctt tgagcgacgc gatgtgctgc gtccgaaact
     3001 cgtgcgtgag tgattcgagc gtgcgcgcga attcggtgtg cacgaagtcc atcgagtcga
     3061 gctcggcgag cacggcgtcc gcggcgacct ggcgcatcgc ggctgcgtta ccgggcaccg
     3121 gtgcatgcgc ctcgacgaca tcgagtggac gcgcgtatgc gtgatcgaga ccgcgctcga
     3181 gcgctgcgct cagcatctgc gtgcgcgcgt tcgccgagag ataccagcga ccgaactgct
     3241 gctgcagctc gccttcgacc gtctccttcg cgccgaacag ccactcgttc gccttgtcgc
     3301 taccgacgag cgcagcggca tcggccttcg ccgccgcagt cgacagccga acatccgcgg
     3361 tgtgcagcgt cgcttgaaac gaaacgtcgt cgacgaatac gccgacttgg cccgcgcatg
     3421 cgttgacctc ggtcgcgacg ccgcagccgc ccagatcgag cgagccatcg gcgaggccgg
     3481 tcaagcacga gcgcacgctc gacgtgcact gcatcggcgg ccacaccgct tcgacgtcgc
     3541 cgctcgtcat gccgagcttc ttgatgccga ggccgagcgt cgcgcgcttc tcgacgccac
     3601 ccgtcgcaaa gtccgcaacg atctggaggt cgccaccggc ggtcagcgcg aacgcctgct
     3661 cgggctgcag atcgacgacg aacttctcgt tatcgatgcg gcgcacgtcg gacagctcca
     3721 cgccatcgac gaccgcacgc acgccggtgt tcgcaccgta ggtgtgtcct tgcacccgat
     3781 acacgacggt gccgccgtac acgacgggcg tcagctccga cacgagatag atcttcgtcg
     3841 agcccgtgaa cgccgcgagg cgaggacgca cgacgacacg cgcggtcagc gaatcgggcc
     3901 gcgagctcga gtgcacgaac gtcatgccga tgaactgctc gacgccatcg acgaggccgc
     3961 gcgcggtcga caccggccac gtgagctcga acgtgcgcgc cgtcttcgca gcgaagtcgc
     4021 cgtagacgtc atcgaagatg aagccgcggc catcggtgag gttgcggctc gtgcggccgc
     4081 gcaatacgta gaggtcaccg cgacgtgcga cggtcttgtt cacccacagg gtggtgtcgc
     4141 cggcgcgaac ggtcagctcg ctcgccgcgt cgactttgga gtcggcggag acctcggcct
     4201 cgtcgacgat cgaatcgcca ccttccgcac acgccgcaac cacaactgcc aaagctacgt
     4261 accgaaacat aggcgcgggg ttgtccgcca tttgtgccga ccgcgcaatg tgttatttcg
     4321 gatgccccgt gtccgaccat cccactgatt ttcaaggctc gattccggat gcgatcacca
     4381 cctcgatcca aggcaagttg cccgacgcga aggtggaggt ctccggcggc ggtggtcact
     4441 ggtccatcgt cgtcgtgtcc gccgcgttcg aaggcaagag catcctcgag aagcagcgca
     4501 tggtgctgtc ctcgatcaag cacttgctcg acggcgcagc cccgccggtc cacgcggtcg
     4561 actcgctcgt tacgaaaacg ccttaacgca ggcggtgcag gccagcgggc cgcttcgcga
     4621 tcgagcgcac cgtcgggttg cagccgagcg ggctgtcgca ctcgagtgtc gtcgacttgt
     4681 cgttcacgag tgccgtgtgc ttcaggctga tgtgcgagtg gtgatggtgg aagtagtacc
     4741 aacctgcgcc accgtcggtc gtctcgtacg cgagcaccgc gttgctgcat gcggccgcgg
     4801 agagccagcc gctgttgcag agctggttca tcgccgagag gatcttcggg ccggctttgc
     4861 cgtcgacgcc gatcacgcgc gtgcgcgagc tctcgaacac gctgccgagg aagaacgcgt
     4921 ggcgccacac gtcgagatac acgggatcgg cgacgcagtg ctgctggtcc tggccgttga
     4981 cgacggtgtt gcagatcggg cgcaggcgat tgtctgccgt gccgatctgg tagtagccga
     5041 gatcgatgtc gtagccgtcg acgtgcgtgc ctgcgggatg accaggcgag ccgatcgacg
     5101 tgccgggaat cgcgccgttg gcttcgctca tgtcgccgag gccgagcggg ccaccgttgc
     5161 ctgcggtcca cgccgcggtc ttgcatgccg tctttgcggt cgcccacgcg acgagcatgt
     5221 tgagatcttt gcgcggatag ctgcggtact ggttcgtcga tgtctcgccg ttgatcgcgt
     5281 agtcgtcgta cgcggtcgtc gtgcgcggcg aaaacggcgt gagcgtgctg catcccgtcg
     5341 tgcaatcgcg cgtcggcaac gagctgcacg tcggtcctgg gcccgcacca gggcctgcgc
     5401 cgacgtccgg tacgcactct ccgctgacgc acgactggcc ggtgccgcac gacgtcgcgg
     5461 tgcacgagca cgcctcgccg cacgtcggat cgcaatcggt ctgcgcggtc ggctcgcacg
     5521 agccatcgct cgcacacgcg ccggcgcaca tatgtgcagc gcaatcgggc cagcagagcg
     5581 agcccgtgtg accgtcgagg ttccagcagc gtgagcccgt cgggcactcg gcctgcgagc
     5641 cggcggcgga gcaattcgtc gacgctttcg tcatgcacac gccgtagatg cagacgccgt
     5701 catggcctgc gtcgtcggca caatccgcgt cgcacgagca atcgctgccg gggccatttg
     5761 cacccgaccc cgagccggag ccagagccag agccagagcc ggtcgcggcg tctacctgcc
     5821 cgttgccgcc gacgtcatcg tcgtcgcccg ttgcattgtt ttgcggacca cacgccgcga
     5881 gcaccaccat caacaagacc gttatccgca cgagcgagca cgtacaacgc ggcgcggcga
     5941 gacgtcaatg ctgaactttg gtcagctgtc cggtgaagtc cgtgaccttg ccggtgagcg
     6001 cgacgagcac ctgcccaccg acggagccgg acggcagccg ctcgcgatcg agacgcgcgt
     6061 acggcgacac gcctgcctcg ccgatcgcat cgacgcgcag ctgcttcgtc agctcgcgac
     6121 cgacgtgcag tgatgcgccg gccgagacgt cgcggggtgg gaacgagaca atagacccat
     6181 cttcgcgggt ccacgtatgc gtgcgcgttg cgaacggtgc gagcgagagc gtggtgcggc
     6241 gcgtgcgccc cgctacgtac gtgacgtcac ccgacacacg agcttcacgc gcgatgttgc
     6301 cgtcgaacgt cggatagaac gatcgcgaca ccgctgcgct cgtcgtcagt cgatcgccgc
     6361 gcacaccacc ttggagcctg ccgacgaaga tcgtcatgtt cggcagaccc tcgctgtcga
     6421 tcgtttcggt ccacgagtcg acgacctcgc cgttgacctt cgtctcgctg ctctgcgtga
     6481 cgctgccgcc cgacatgccc cagccagcgg ccgcttgcac gaacacgacg ccgtcgtcgt
     6541 agctcacgcg tgctggatag aacgcgctcg cgacgccgcc gcgattgtcg atacctgctt
     6601 tgagcgcgtt ggattcgagc acggcggcat cgatctgaag tccttcgacg cggcgatagc
     6661 gatatgcggc aaggtcgaga tccagcgtga cgatcgtgcg cgactcgctg ccgtcgacct
     6721 gcgtcgtctc gccgtggccg aagatcatcg gcagaatcgt atggcgcgcg ttcggattgc
     6781 gccggttgta gagctcaccg gctccgaatt cgatctgatc gtacgcagtt gcatagcgtc
     6841 ggctccacag cgagcgacga tccgaaagac tgggcgcaag gccgatgccg cgcgtgccgt
     6901 cgaacgcgat cgtgaacgcg acccgcgaca gacaagcgcc gtaatcgacc tgtcccgtca
     6961 catgctcgtc gtgcgcgaac gggcgaaatc gtccctgcgc cgagccgtgc gcgatatcgc
     7021 aaccgatgcc tccgatcgcc atcgctgcac ccgccgctgc accaccacca ccttcgccgc
     7081 cacctgcgag ctgcgcgaat gccgccgagc gtacgtaggg atcgagcttc gcctcctcgg
     7141 ggatgtcacg gctcatgccg agcacgagga tcggcagctc atcggtgacg tcacgtcgtc
     7201 gaccgagctc gagctgggag accgcgagct cgtcctcggc actcggggcg gcataggcag
     7261 agccggcgaa tcccagcacg aggagcgtta ggcgcatgcg catcggtcgc gcgaattgca
     7321 acgcctgcgc cgcgtgcaag tgcgcgaaac acagcgttgc gatgtcgaga ttccaccgcc
     7381 tgcctgtcgg gagtcagcga gttctggtct aggctccgcc gcatgaagaa gcttggagtt
     7441 gcaatcgttc tcctcgttgc cgcgctcgcg ggatgcaaca agaaggcagc cgacaaggct
     7501 ccggagacca acgctggctc gggcgcgacg acgacgccgc aaacgggctc ggcaggctcg
     7561 gcaggcatgg cgggctcggc aggctcggcg gcggaagtgg cgtgggacgg caagacgccg
     7621 ctctcgctcg aaggcttcca gacgcccgag tcggtcctct acgacgccga tggcgacggc
     7681 tacctcgtgt cgaacatcgg gggccagccg ctcgacctcg acgacaacgg cttcatctcg
     7741 aagatctcgc ccgacggcaa gatcaccgag ctcaagtgga tcgacggcgc gaaggacaac
     7801 atcaagctga acgcgcccaa gggcatggcg atcaccggcg gcctgctcta cgtcgccgac
     7861 atcgacaccg tgcacaagtt cgacgcgaag accggcgagc tcaagggcga catcaagatc
     7921 gacggcgcga cattcctcaa cgacgttgca gttgcgcccg atggcggcgt gtacgtgacg
     7981 gacagcggcc tcgacaaaga gttcaagggc accggctccg acgcggtcta catgatcggc
     8041 aaagacgaca aggtcaagcc ggtcatgaaa gacaagaagc tcgcggcgcc gaacggcatc
     8101 accgccggcg acaagggcgc ggtctgggtc gtgacgtttg gctcgggcga gatctacgaa
     8161 gtcgacgcga agggcaagca gggcaagaac cagaatctgc cgaagggtca gctcgatggc
     8221 gtcgtcgtcc tcgacggtgg cgaggtactg gtgtcgagct gggaaggctc ggcgatctat
     8281 cgcggcaagc ccggtgcgga gtggaagccc gtcgtcgaga acgtgaagtc gccggccgat
     8341 atcggctacg acacgaagcg gcaccgcatt ctgattccgg tgttcatggg caacaccgtc
     8401 atcattcagc cgactgaatg attccgtgga aagagctcgg ccgcgcacgc gtgggcaaac
     8461 gagatctcgt gctcgcgcgt cgcggagatg aatttgcgct gcgcgtcggc ggcgccgagc
     8521 tgatgaacag tcgccagcac tcgagcgaag aagcgctcgc gaagctcggc tgtgcgacgc
     8581 tcggcagcgt cccgcatgca cgcgtgatga tcggcgggct cggcatgggc ttcaccgcac
     8641 gtgccgcgct cgacgtgttg ccaagcgacg cgcgcgtgac gattgtcgag ctcgtgcccg
     8701 aggtcgtcgc gtggaatcgc gagcatctgg gcgacctcgc cggtcgaccg ctcgacgatc
     8761 cacgtgtgac ggtcctcgag tgcgacgtca cgcaagtgat tcgcgagagt gccgagacgt
     8821 ggaacgcgat cctgctcgac gtcgacaacg gtcccgatgc gttcacgtcg ccggacaacg
     8881 cgaagctcta cggcctcaag ggcctcatgg catgtcgcaa ggcgctcgtc cgcggcggca
     8941 tgctcggcgt ctggtctgtc gagaacgaca atgcgttcac cggtcacctg accgcagccg
     9001 gcttcgacgt ggacaagcag cgagtgccgt cgcgaccggg ctcgagcgta aagcacgtcg
     9061 tctggatcgc gcgcaagaag tagctagcga cgacgacggc gaagcacgag cgcgagcacg
     9121 ccgagcgaga cgagaccgct gccaccggcg gaaccgccat cgcagcagcc gccgccacca
     9181 gcgaagatcg gatcttcctc gggaggaggc tcttcgccgc acaagccatc gtctggcagc
     9241 tgcgcgggaa acggcgcgcc gtcgataccg gtgagcgcga cgtcgtcgat gtcccagccc
     9301 tgcgcggcgg tcgcctcgtc agtgccgagc cggaaccgaa tgcggaatgt ctgatccgca
     9361 agcgccgttc cgagatcgag cgtgatcgtg tccatcgcgg ggtacgacgc gttcttgccg
     9421 gtgaaggcag gcttgccggt gagcgcgttc gcggtatcgg cttgcagcac gtcggtgtac
     9481 ggcacgttcg tgagcgtcga gaggtcttgc cagatctcgt cgccgccgag cgagtactcg
     9541 atgacgccgc cgtcgtacgc gatgccgtcg gtgacctcga gcgagtggcg gtgcttgaac
     9601 gtcagcgtga cgggctcggt cgcagagcca tggagatacg gcgacgtcag gcgcgtgtcg
     9661 gaatcgagct cgaggtcgct ggcgtgccag tagccgttga gcggcgacga ccggcgatgc
     9721 gtccacgcgg ccgtccaggg aacccagacg gactccagcg cgtcgaagct gtcggtcgtc
     9781 gaggcggcag ggacgtcatc gacgttgagg cgtgccacga tcgggaatgc ggtgacggtc
     9841 tcgcatgcac cgtcgccgct gacttcgaga gacagcgtgc cttcgatcgc ttcggtgtga
     9901 ccggcttcga tcgcgacatc gacgggcagc tcgaccgact cgctcggttc gaggcgtccg
     9961 atcgcgcgtg gcgtgttgtc gacatcgatc gttcggtcgc tcgacgtcac gcgcacggag
    10021 acgttcgtca acgccgcgtg gccgcggttc gcgacttgga tgcggatgtg cgcggtctcg
    10081 ccgctgtcga gcacgccgtc gtgatcacac gcatcgtcga gctcgagcgt ggcgacatct
    10141 gcgcgaccgg cgacgaggaa gctctccttg aggccggtga agtcggtgct cgccggaggc
    10201 ggcgcaatcg cacagctgcc gaagccgcgt gatgcaaatg cgtcgagcat caggccgtgg
    10261 tcgtacgagc tcgcgccttc gacggccgag aggatcgcat cgcgcgcttc catgggcgtt
    10321 gcctccgtcg gtgtcagcgc aaggcccgcg acgacgtagc gcgccatctt cgcgcgagtc
    10381 tcgaggaagt cagtgctcga tttctgcagc gccgcgtaaa cctgccagag cgccgacgcc
    10441 cagatctcgc cggcgttgtg cacttcggag ttgctgttca tgacgaggaa cggatggccc
    10501 gacggtggcg cctcgccgct cgccatgtgg cggaacgaca gcgcgttgat
//
LOCUS       DATLBZ010000114         8691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5328714, whole genome shotgun sequence.
ACCESSION   DATLBZ010000114 DATLBZ010000000
VERSION     DATLBZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5328714"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            869..2017
                     /locus_tag="VMZ53_06070"
     CDS             869..2017
                     /locus_tag="VMZ53_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28052.1"
                     /translation="MRTLAVGALALLAGGCLEAHATRCANGAICPDGFACTENAETLC
                     GPELLVEACMGRDEFAPCEVHVSGEPAVGPGACHAGVCDACTEDFAGCHFAQWTAMRS
                     PTTENLYTVFAASDSDVDAAGDNGTFLHYDGKAWSRSESYKQLVGAEDAETIWRDATS
                     ELVLVTKGDIYRKRIDGSWELAVSTGSTLSAMWAASPTDVVAAGLAGVIVEYNGTTAT
                     PVTPAPIPSFVYRGVWVSSTGQAWAVGRQGGVVRRVNGVWSVARAPMSSQPDLRAVWG
                     RADNDVWMVGDVPAVGATEPTILHFDGTTIAPAALPTGTLGPIQLLGLTGTATDVWAA
                     SAGSTIEHYDGTTWTQMETSAGASLDAISAAHDIFAVGVGGRIYRFTP"
     gene            complement(2018..3607)
                     /locus_tag="VMZ53_06075"
     CDS             complement(2018..3607)
                     /locus_tag="VMZ53_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28053.1"
                     /translation="MSLTRAVPLALLVSACALGGDDGSSISQAISGTASINVNSSSVA
                     VTLTSDAQWSLTKTGSVSGNTVTWNVTATKTATVSGQLVVQGHLNVTNSGSGPATLGN
                     IVVNLQTRVSNTWKSASADVANGTLGDAATTAKIHAAASSENKSSFTENAASGKLEFM
                     DATNNTVFSLIPEVTINAGQTRSLLFQATFDNNVLHLATGTPIRAEVIVSFGNATATG
                     NSTPNVDINGNGLIDWDEKRVRSVPTRLSVTVPPTTNGNTTPTLKDTASDIIGEGVTV
                     GSTWFNLGPTGGTVVAQVSGVGTVTNCAHLTSPGQTVTSGGFTFQQVGAINLQACNTQ
                     TVSAPPACTHGTPNCAWASNDFRIFTQTSWEGNGKSTLEASYDNVYASTFGTLTIGTT
                     GGPTASWTSVQAIENYLPQAGSPAPLDSTSVDATSTSSHAFGGEAVALSLNIAFSDAN
                     VTPHYQTLAFGDVRVCGTSTAADGQTVRGVLALTNSLLSFGPQPSIDALYPVLLQLNA
                     SFDNGTVSTWAQEHLVNGACP"
     gene            3843..4313
                     /locus_tag="VMZ53_06080"
     CDS             3843..4313
                     /locus_tag="VMZ53_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HUS28054.1"
                     /translation="MHLDPTSTLASGHPLIDIDLTVLVQFGLFLVLFFVSNKLLFQPY
                     LQLRERRKAGIDGARAEADRMTASADAKLADYEKQLAAARSRANEEGRKVRAEASAHE
                     KDVTEKARGEAQKAIDEATAKMRQQTEAARLQLLPQANTLARQIASKLLGREVA"
     gene            4314..5159
                     /locus_tag="VMZ53_06085"
     CDS             4314..5159
                     /locus_tag="VMZ53_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HUS28055.1"
                     /translation="MMKKLLIIASLLFAPTAFAQDAVEQHSDEHLGGVDDTTGGENAA
                     GTQGGEAHGHDPTEHFNFFNFGYRGHDQWGDKFGDGVMRDPKTGAIATETHTKVIVDP
                     ETGKKKTVHEAEPEEEEAMSPPFVLMLLNFAILLFILAKFGGPVARKAAEERHDQIKT
                     ALDEAAKLRDQAAKKLAEYETRIKDVDSEVKKLVEGIRADAEADKKRILEAAAVQAAT
                     MKRDAELRIAAEIELARAQLTKEVTAAAAGATEKLLREKVTNDDQNKLVTTFITNVAA
                     QKEAR"
     gene            5159..5686
                     /gene="atpH"
                     /locus_tag="VMZ53_06090"
     CDS             5159..5686
                     /gene="atpH"
                     /locus_tag="VMZ53_06090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HUS28056.1"
                     /translation="MLARRYAKAIFDIGGEQGGLDKMGADLRTLANAMKTSPELVSTL
                     SNPAIRRGDRRKIIDALLQRIGVVTTTRNTVYLLLEKERLGALPDISRQLDAMIEEKA
                     GRVAAEVVSAKPLDRSQLSQITATLEKLSGKKVSVQTREDPDLLGGVVAKVGDTVYDG
                     SLRSQLRNLRDELSK"
     gene            5699..7234
                     /gene="atpA"
                     /locus_tag="VMZ53_06095"
     CDS             5699..7234
                     /gene="atpA"
                     /locus_tag="VMZ53_06095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HUS28057.1"
                     /translation="MDIRAAEISDIIRKQIENYDKKVSVTETGTVLTAGDGIARVYGL
                     SGALAGELLEFEGAGGTTVQGMVLNLEEDNVGVALLGSFESVREGDTVKRTGRIVEVP
                     VGEELLGRVVNAMGFPIDGGKALTGKTKRQVEIKAPGIIARKSVHEPMQTGMKAIDSM
                     IPIGRGQRELIIGDRGTGKTAIAIDTIINQKGQNMFCFYVAIGQKQSTVATVVDRLAK
                     AGAMEYTTVVVAGASEPAPLQFIAPYTGVTMAEYFRDSGRHALIVYDDLSKQAVAYRQ
                     LSLLLRRPPGREAYPGDVFYIHSRLLERAAKMSDAKGAGSLTALPIIETQAGDVSAYI
                     PTNVISITDGQIFLEADLFFSGVRPAVNVGISVSRVGGAAQTKAMKKIAGRLRLELAS
                     YREKAAFAQFGGDLDKATLQQLARGERMVELLKQGQFVPMSMDEQVIVIYAGTNGFID
                     DYPVSVLGRYEKELLSFMKSRKQSLLDELKSTGKLEGDTEKQLRDALTEFAKQFSTDE
                     KKA"
     gene            7241..8143
                     /gene="atpG"
                     /locus_tag="VMZ53_06100"
     CDS             7241..8143
                     /gene="atpG"
                     /locus_tag="VMZ53_06100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HUS28058.1"
                     /translation="MASLKAIRKRIVSVKNTRKITKAMKLVAAAKLRRAQDTIIAARP
                     YSSALSSVVSDLSGVAGKDSHPLFEERDVKRAAIVVITADRGLAGAFNTNVIKAVERY
                     SQNELSGATEITLRIIGKKANQHFSRRNATIASYDPAPTSANALQVAREAANRAIADF
                     QDNKVDRVFLAYNEFKNAGSQVVRVVQILPVSPGSPDGSGTALVKGDKAPAQTTTDYV
                     YEPNKQVLLDRLVPLYVQIQIYRATLESIAAFFGAQMMAMENATKNAGDMINRYTLQY
                     NRARQAAITKELAEIVGGAEALKG"
     gene            8176..>8691
                     /locus_tag="VMZ53_06105"
     CDS             8176..>8691
                     /locus_tag="VMZ53_06105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831129.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HUS28059.1"
                     /translation="MGRVVQVIGPVVDVAFDSAELPEINTALLLTNKAIDDRVDNLTV
                     EVAQHLGEKMVRCVAMDTTDGLVRGQPVKNTNAAISMPVGPEVLGRILNVVGNPVDEA
                     GPINAKQRLPIHRLAPKFTDQAVNVEMFETGIKVIDLLAPYRKGGKIGLFGGAGVGKT
                     VLIMELINNTAK"
BASE COUNT         1584 a         2821 c         2842 g         1444 t
ORIGIN      
        1 tgcacagagc gccgggccgg cggggagcgg cgcgcccggc ctttcaacgg ccagggcgcg
       61 cgccgcgacc tcgccgccgg cccaggcgcg gctcgctcgt tcgcgcttcc cattgcgaac
      121 gggtcgcggc attcgtgctg ccgaatcgcc gcacgcttca tcgtcgcgat ccgccgaagg
      181 cggatgctcg tctgcgcgat gcccacgtgg gcacggcact cgtcgacgtg tccatcgcga
      241 tgtgcccacg tggcacggaa gctggtcggc ggagatcgta gcgatgtgcc cacgtggggc
      301 acggaagctg gtcggcggat gtcgtcgcga tgtgcccacg tgggcatgga agctggtcgg
      361 cggggatcgt agcgatgtgc ccacgtgggc acggcactcg tcgacgctgc cgagcgcaac
      421 ggtcatcgcg atgtcgaggc agcatgcttc ttctccggcg tgctgcacag agcgccgggc
      481 cggcggggag cggcgcgccc ggcctttcaa cggccagggc gcgcgccgcg acctcgccgc
      541 cggcccaggc gcggctcgcg cattcgcgct tcacatcgcg aacgggtcgc ggcattcgtg
      601 ggatcgccgc acgcttggtc gtcgcgatcc gccgaacgcg gatgctcgtc tgcgcgatgc
      661 ccacgtgggc acggcactcg tcgacgctgc cgagcgcaac ggtcatcgcg atgtcgaggc
      721 accacgcttc ttctccgacg tgcccaggcg cggctcgcgc attcgcgctt gccattgcga
      781 acggtcgccg aatcgccgca cgcttcgtcg tcgcgatccg ccgaacgtgg gcacgcatcg
      841 ctccgccagt ccctctataa tgatgcacat gcggacgctc gccgttggtg cgctcgcctt
      901 gctggcaggt ggctgcctcg aggctcacgc gacacgctgc gcgaacggcg ctatttgccc
      961 tgacggcttc gcgtgcaccg agaacgcgga gacgctctgc ggccccgagc tcctcgtcga
     1021 ggcttgcatg ggtcgcgacg aattcgcgcc ctgcgaggtc cacgtttcgg gagagcccgc
     1081 ggtcgggccg ggcgcgtgcc atgcgggcgt ttgcgatgcg tgcaccgaag atttcgccgg
     1141 ttgccatttc gcgcagtgga ccgcgatgcg gtcaccgacg accgagaatc tctacaccgt
     1201 ctttgctgca tccgattccg atgtcgatgc ggccggcgac aacggcacgt tcctgcacta
     1261 cgacggcaaa gcgtggagcc ggtccgagtc gtacaaacag ctcgtcggcg cagaggacgc
     1321 agagacgatc tggcgagacg cgacgagcga gctcgtgctc gtcacgaagg gcgacatcta
     1381 tcgcaagcgc atcgacggca gctgggagct cgcagtctcg acgggctcga cgctgtcggc
     1441 gatgtgggcg gcatcgccga cggatgttgt tgcggccggg cttgccggcg tcatcgtcga
     1501 gtacaacggc acgaccgcaa cgcccgtgac acccgcaccg attccgagct tcgtgtatcg
     1561 cggcgtctgg gtgagctcga ccggacaagc gtgggccgtc gggcgtcaag gcggagttgt
     1621 gcgtcgcgtg aatggtgtgt ggagcgtcgc gcgtgcgccg atgtcttcgc agcccgatct
     1681 gcgtgctgtg tggggccgcg ccgataacga tgtgtggatg gtcggcgatg tgccggcggt
     1741 tggcgcgacc gagccgacca tcctgcactt cgacgggacg acaatcgcgc ctgcagcatt
     1801 accgaccgga acgctcgggc cgatccagct gctcggcctc acgggtacgg ccacggatgt
     1861 gtgggccgca tcggcgggct cgaccatcga gcattacgac ggcacgacgt ggacacagat
     1921 ggagacgtcg gcgggggcat cgctcgacgc gatctccgcg gcgcacgaca tctttgccgt
     1981 cggcgtgggc ggtcgaatct atcgcttcac gccgtgatca gggacaggca ccgttgacga
     2041 ggtgctcttg cgcccacgtg ctcacggtgc cgttatcgaa cgacgcgttg agctggagga
     2101 ggaccggata gagcgcatcg atcgacggtt gcgggccaaa gctcagcagc gaattcgtca
     2161 gcgccagcac tccacgcacg gtctggccat ccgcggccgt cgacgtaccg cacacgcgga
     2221 catcgccgaa cgcgagcgtc tgatagtgcg gcgtcacgtt ggcgtcggag aaggcgatgt
     2281 tcagcgagag cgcgactgct tcgccgccga acgcgtgcga tgacgtactc gtcgcgtcga
     2341 ccgacgtcga gtcgagcgga gcaggagagc cggcctgcgg aagataattc tcgatcgcct
     2401 gcacgctcgt ccatgacgcc gtggggccac ccgtcgtgcc gatcgtcagc gtgccgaacg
     2461 tcgatgcgta aacgttgtcg tagctcgcct cgagcgtcga cttgccgttt ccttcccacg
     2521 acgtctgcgt gaagatccgg aagtcgttgc tggcccacgc gcagttcggc gttccgtgcg
     2581 tgcacgccgg cggtgcgctg accgtctgcg tgttgcacgc ttgcagattg atcgcgccga
     2641 cctgctggaa cgtgaaaccg ccgctcgtga ctgtttgacc gggcgacgtc aggtgcgcgc
     2701 agttcgtcac cgtgccgaca cccgagactt gcgcgacgac cgtgccgccc gtcgggccga
     2761 ggttgaacca cgtgctaccg acggtgacgc cctcaccgat gatgtcggag gccgtgtcct
     2821 tgagcgttgg cgtggtgttg ccattcgtcg tcggcgggac cgtcacggag aggcgcgtcg
     2881 gcacgctgcg cacgcgcttc tcgtcccaat cgatcaagcc attgccattg atgtcgacgt
     2941 tcggcgtgct gttaccggtc gccgtcgcgt tgccgaacga cacgatcact tcggcgcgga
     3001 tcggcgtgcc cgttgcgagg tgcagaacgt tgttgtcgaa cgtcgcttgg aagaggagcg
     3061 agcgcgtctg gcccgcattg atcgtcactt cggggatcag cgagaacacg gtgttgttcg
     3121 tcgcgtccat gaactcgagc ttgccggacg cggcgttctc ggtgaacgag ctcttgttct
     3181 ccgagctcgc cgccgcgtgg atcttcgccg tcgtcgcggc gtcaccgagc gtgccgtttg
     3241 cgacgtccgc cgatgccgac ttccacgtgt tgctcacgcg cgtctggaga ttgacgacga
     3301 tgttgccgag cgttgcggga cctgaaccgg agttcgtgac gttcagatgg ccttgcacga
     3361 cgagctgacc actgacggtt gcagtcttcg tcgccgtgac attccacgtt acggtatttc
     3421 cgctgaccga acccgtcttc gtcagcgacc actgcgcgtc actcgttaat gtgaccgcga
     3481 ccgatgacga gttgacgttg atgctcgcgg tcccggagat cgcttgcgag atcgaactgc
     3541 catcatcgcc accgagcgcg catgccgaga cgagaagcgc gaggggtacg gcccgggtaa
     3601 gcgacatggc gggaccataa aacgagcttt gggatacgtg gcgccaggtt ctgcccaaaa
     3661 cgcgcaacat tcgtacaccc ggaactgagg acgcgtcagc gggagtcgcg agcggcgcga
     3721 gatcgcggcg caatgtcgca gcacgcgagg gcaaagctgc ggcatcccag cgttgacacc
     3781 gcatagtgcc gagactataa gcaccgcggt cgcaccaagc gccgagattt cgcaaggttg
     3841 ccatgcatct ggaccccact tcgaccctcg cttccggtca cccgctgatc gatatcgacc
     3901 tgacggtcct cgttcagttc gggctgttcc tggtgctctt tttcgtctct aacaagctgc
     3961 tgtttcagcc gtatctgcag ctgcgcgagc ggcgcaaggc ggggatcgac ggggcgaggg
     4021 cagaggccga tcgcatgacg gccagcgcgg acgcgaagct cgcggactac gagaagcagc
     4081 tggcggcggc acgcagtcgc gccaatgaag agggccgcaa ggtccgcgcg gaagcgtcgg
     4141 cgcacgagaa agacgtcacc gagaaggccc gcggcgaagc gcaaaaagcc atcgacgaag
     4201 ccaccgcgaa gatgcggcaa cagaccgaag cggcgcgtct gcagctgctt ccgcaggcga
     4261 acacgctcgc gcgtcagatc gccagcaagc tcctcggccg ggaggtcgcc tagatgatga
     4321 agaagctcct catcattgcg tcgctgctgt ttgcgccgac ggcgttcgcg caggacgcag
     4381 tcgagcaaca ttccgacgag cacctcggcg gtgtcgacga cacgactggc ggcgagaacg
     4441 ctgcgggtac gcaaggcggc gaagcgcacg gccacgatcc gaccgagcac ttcaacttct
     4501 tcaacttcgg ctatcgcggc cacgatcagt ggggcgacaa gttcggcgac ggcgtgatgc
     4561 gcgatccgaa gacgggcgcc atcgcgacgg agactcacac gaaggtcatc gtcgatcccg
     4621 aaaccggtaa gaagaagacg gtccacgagg ccgagcccga ggaagaagag gcgatgtcgc
     4681 ctccgttcgt cctcatgctg ctgaactttg cgatcctgct cttcatcctc gcgaagttcg
     4741 gcggaccggt cgcgcgcaag gcggccgaag agcgccacga tcaaatcaag accgcgctcg
     4801 acgaagccgc caagctccgc gatcaagcgg cgaagaagct cgccgagtac gagacgcgca
     4861 tcaaggacgt cgactccgaa gtgaagaagc tcgtcgaggg cattcgcgcg gacgccgaag
     4921 ccgacaagaa gcgcatcctg gaagcagccg ccgtgcaggc agccacgatg aagcgcgacg
     4981 ccgagctgcg catcgcagcc gagatcgagc tcgctcgtgc gcagctcacg aaggaagtca
     5041 ccgcggccgc cgcgggcgcg accgagaagc tgctccgcga gaaggtcacc aacgacgatc
     5101 agaacaagct cgtcacgacg ttcatcacga acgtcgccgc gcagaaagag gctcgctgat
     5161 gctcgcccgt cgttacgcga aagcgatttt cgatattggc ggcgagcagg gcggcctcga
     5221 caagatgggc gctgacctcc gcacgctcgc gaacgcgatg aagacgtcgc cggagctcgt
     5281 gtcgacgctc tcgaacccgg cgatccgccg cggcgaccgt cgcaagatca tcgacgcgct
     5341 gctgcagcgc atcggcgttg tcacgacgac gcgcaacacc gtctatctcc tcctcgagaa
     5401 ggagcgcctc ggtgcgctgc ccgacatctc gcgtcagctc gacgcgatga tcgaggagaa
     5461 ggccggtcgc gtcgccgccg aggtcgtatc ggccaagccc ctcgatcgct cgcagctgtc
     5521 gcagatcacg gcgacgctcg agaagctgtc tggcaagaag gtcagcgtgc agacgcgcga
     5581 ggatcccgat ttgctcggtg gcgtcgtcgc gaaagtcggc gacacggtct acgacggttc
     5641 acttcgttcc cagctccgca acctccgcga cgagctctct aagtaaggac acggcaccat
     5701 ggatattcgc gccgcagaga tttccgacat catccgcaag cagatcgaga actacgacaa
     5761 gaaggtctcg gtcaccgaga cgggcaccgt gctcacggcc ggcgatggca tcgcgcgcgt
     5821 gtacggcctg tcgggtgcac tcgccggtga gctcctcgag ttcgagggcg ccggcggcac
     5881 gacggtgcag ggcatggtgc tcaacctcga ggaagacaac gtcggtgttg ctctcctcgg
     5941 ctcgttcgag tcggtccgcg aaggcgacac cgtcaagcgc accggccgca tcgtcgaagt
     6001 tccggtcggc gaagagctgc tcggccgcgt cgtcaacgcg atgggcttcc ccatcgacgg
     6061 tggcaaggcg ctcacgggca agacgaagcg ccaggtcgag atcaaggcgc cgggcatcat
     6121 cgctcgcaag agtgtccacg agccgatgca gacgggcatg aaggcgatcg actcgatgat
     6181 tccgatcggt cgcggtcagc gcgagctcat catcggcgac cgcggtaccg gcaagacggc
     6241 catcgcgatc gatacgatca tcaatcagaa ggggcagaac atgttctgct tctacgtcgc
     6301 catcggccag aagcagtcga cggtcgcgac ggtcgtcgat cgcctcgcga aggcgggcgc
     6361 gatggagtac acgacggtcg tcgtcgctgg cgcgtcggag cccgctccgc tgcagttcat
     6421 cgcgccgtac accggcgtca cgatggccga gtacttccgc gactcgggcc gccacgcgct
     6481 catcgtctat gatgatctca gcaagcaggc tgtcgcgtac cgccagctgt cgctgctcct
     6541 ccgccgcccg ccgggccgcg aagcgtatcc gggcgacgtc ttctatattc acagccgcct
     6601 cctcgagcgc gccgccaaga tgtcggacgc caagggcgcg ggctcgctga cggcactgcc
     6661 gatcatcgag acgcaagcag gcgacgtctc ggcgtacatc ccgacgaacg tcatctcgat
     6721 caccgacggt cagatcttcc tcgaagctga cctcttcttc tcgggcgttc gcccggccgt
     6781 caacgtcggc atcagcgtct cgcgcgtcgg tggcgccgcg cagaccaagg cgatgaagaa
     6841 gatcgccggt cgcctccgcc tcgagctcgc gtcgtatcgc gagaaagcgg cgttcgcgca
     6901 gttcggtggt gacctcgaca aggcaacgct gcagcagctc gctcgcggtg agcgcatggt
     6961 cgagctcctc aagcagggcc agttcgtccc gatgtcgatg gacgagcaag tcatcgtgat
     7021 ctacgccgga acgaacggct tcatcgacga ctacccggtc tccgtgctcg gccgttacga
     7081 gaaggagctg ctctccttca tgaagtcgcg caagcagagc ctcctcgacg agctgaagtc
     7141 gacgggcaag ctcgaaggcg acaccgagaa gcaactccgc gacgcgctga ccgagttcgc
     7201 caagcagttc tcgaccgacg aaaagaaggc gtaaccagcc atggccagcc tgaaggcaat
     7261 ccgcaagcgc atcgtgagcg tgaagaacac gcgcaagatc acgaaagcga tgaagctcgt
     7321 cgcggctgcc aagctgcgtc gcgcgcagga cacgatcatc gcggcgcgtc cgtactcgtc
     7381 cgcactgtcg tcggtcgtgt ccgacctgtc gggtgttgct ggaaaagact cgcatcctct
     7441 gttcgaggag cgtgacgtca agcgcgcggc gatcgtcgtg attaccgccg accgtggtct
     7501 cgccggtgcg ttcaacacga acgtcatcaa ggcggtcgag cgctactcgc agaacgagct
     7561 gtctggtgcg accgagatca cgcttcgcat catcggcaag aaggcgaacc agcacttctc
     7621 gcgtcgcaac gcgacgatcg cgagctacga ccccgcaccg acgagcgcga acgcgctgca
     7681 agtcgcgcgc gaggccgcga accgcgcgat cgcggacttc caggacaaca aggtcgaccg
     7741 cgtcttcctc gcctacaacg agttcaagaa cgcgggcagc caagtcgttc gcgtcgtcca
     7801 gatcctcccg gtgtctccgg gctcgccgga tgggagcggc acggctctcg tcaaaggtga
     7861 caaggcgccg gcgcagacga cgaccgacta cgtctacgag cccaacaagc aggttctgct
     7921 cgaccggctc gtcccgctgt acgtccaaat ccagatctat cgcgcgacgc tcgagtcgat
     7981 cgctgcgttc ttcggcgccc agatgatggc gatggagaac gcgacgaaga acgccggcga
     8041 catgatcaat cgctacacgc tgcaatacaa ccgcgctcgt caggcggcga ttacgaagga
     8101 gcttgccgag atcgtcggcg gcgccgaggc tctcaagggg taacgaacat ggctaccgat
     8161 ttctctccca attccatggg ccgcgttgtt caggtcatcg gacccgtcgt cgacgttgcg
     8221 ttcgactcgg ccgagctgcc cgagatcaac acggcgctcc tcctcacgaa caaggcgatc
     8281 gacgatcgcg tcgacaacct caccgtcgag gtcgctcagc acctcggcga gaagatggtt
     8341 cgctgcgtcg cgatggacac gacggacggt ctcgtccgcg gccagccggt gaagaacacc
     8401 aacgccgcga tctcgatgcc ggtcggcccg gaagttctcg gccgcatcct gaacgtcgtc
     8461 ggtaaccccg tcgacgaagc gggtccgatc aacgccaagc agcgtttgcc gatccaccgc
     8521 ctcgcgccga agttcacgga tcaagccgtc aacgtcgaga tgttcgagac gggcatcaag
     8581 gtcatcgacc ttctcgctcc ctatcgtaag ggcggcaaga tcggtctgtt cggtggtgcc
     8641 ggcgtcggca agaccgttct gatcatggag ctcatcaaca acaccgcgaa g
//
LOCUS       DATLBZ010000115         9099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8253732, whole genome shotgun sequence.
ACCESSION   DATLBZ010000115 DATLBZ010000000
VERSION     DATLBZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8253732"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VMZ53_06110"
     CDS             complement(<1..1158)
                     /locus_tag="VMZ53_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28060.1"
                     /translation="MADGDAAGGTLPGPGAPAAPAATPAAAPASPAPPAPLAPPAGMP
                     AAIPGVTPAAAPIGTDETIAPVAKPAANIATDGTLPGVAAGPSPAAHPAAAAAHPAAA
                     PAHPAGAPTHPAAAPTRPAAAPSPAFAATLAGSTVAAVGANAAAIGAKAAAAVGAKVG
                     ANAAAFAATAAGSTAGADAAHSETLPAQGGDTDRDGGRGGGGAHAATLPGGYTVADLP
                     SLPRVDDQTYAIGVEIARGGMGKILSARDRRLRRDVVIKVMKRDGGMIDPRFEREALI
                     TARLQHPSIVRVYDAGVLGDGRAFYAMERVRGRSLEAIIAESKTLAERMALLPHAIAV
                     CDAIAYAHSESVLHRDLKPSNVLIGPFGETVVIDWGLAKEIRDEDGQLASDS"
     gene            complement(1219..1812)
                     /locus_tag="VMZ53_06115"
     CDS             complement(1219..1812)
                     /locus_tag="VMZ53_06115"
                     /inference="COORDINATES: protein motif:HMM:NF042873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease superfamily protein"
                     /protein_id="HUS28061.1"
                     /translation="MATPTKPPSRSKLIASRMLAQPKGAIAALKAGAAPNLSKQTLTA
                     MTGNGYRDLIASYIHENYAPHGLDVYLEISLGKTIIGKDRFIDIFVVRPEDRKAIAIE
                     CKYQDSQGTVDEKIPYALADLEALWMPGLLVYAGSGWSKGVMHSLEASRLAAYCLPDA
                     TTLGRSRETRELDHILASVFGFWDLVLPPSKKLRRAP"
     gene            complement(1939..2676)
                     /locus_tag="VMZ53_06120"
     CDS             complement(1939..2676)
                     /locus_tag="VMZ53_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HUS28062.1"
                     /translation="MDQPRFQLTQGDAVAWLRTLPASSVDLVITDPPYESLEKHRAIG
                     TTTRLKHSKASSNDWFSIFPNARFPELFAEVFRVLKKDTHFYLFCDPETMFVAKPLAE
                     EAGFRFWKPLIWDKVRIGMGYHYRARYECVLFFEKGKRKLLDLGIADIIEAPRINNGY
                     PAEKPPEVSEILIKQSTDAGDLVIDPFMGSGSVGLASIRNKRNFRGNDLCAEALEITQ
                     QRLLEAGGAEGELPLRVTSQPQLGLTI"
     gene            2783..3592
                     /locus_tag="VMZ53_06125"
     CDS             2783..3592
                     /locus_tag="VMZ53_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28063.1"
                     /translation="MASDADGDDDFGGLARDLETMSRRRVLELVTKAAAGITLAPIIG
                     ACGDNGADVMRDANAVGDSASDQCARIPGETPGPYPGDGTNGPNALTIAGINRSDIRT
                     SIGSASGTATGIELAVTLTVVNAATCEPLVGYFVYLWHCTQAGDYSMYTGAAQSENYL
                     RGVQATDSAGKVTFTTIFPGCYPGRWPHIHVEILSGSMTKVAVSQLALPDATCDAVYA
                     TAGYEASATTFAQTSLANDSVFRDGSSRQVVSVTGAVGSNLAGALTIAIAV"
     gene            complement(3589..4353)
                     /locus_tag="VMZ53_06130"
     CDS             complement(3589..4353)
                     /locus_tag="VMZ53_06130"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HUS28064.1"
                     /translation="MLKKTLAALALATSLTTVAACNDRPQVIAVQAPDPKAPSKQPGG
                     MTVNGQATLEVSPDCADLTITITADSMRPGTATKDLETKKQLLIAALLKAGVETNDVK
                     LSTLSLDPVYEQSKEGWATLKIHTYRAQITVTATTRDFSKIADILDSAGQAGASSMST
                     QFRRSDMASLKKQVREDALKAAKEKAEQTAKTLGIKLGRITAVAENQGGYMWSATYFP
                     QVANSVDVRNSGVALGGTLQPLTLDVTISYELATET"
     gene            complement(4440..5066)
                     /locus_tag="VMZ53_06135"
     CDS             complement(4440..5066)
                     /locus_tag="VMZ53_06135"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUS28065.1"
                     /translation="MSFGGRDRLYGMQLYYHPRSPYSHKVMIALAEKRVAYEPIMIRP
                     DDAQFAKLTPIKKVPLLVLADGWKIPESTIIIEYLDTHFSTGTRLIPDDRDLARQTRF
                     YDRIADLYVTEPLMTLLFKRGDADAAHTRLDAMLAGLDEHISKRPWIMGDELTMADCS
                     FIPALRYARELHPFDRHKHVVAYMSRALERPSVRSVFEQVAPHLEKSA"
     gene            complement(5063..5983)
                     /locus_tag="VMZ53_06140"
     CDS             complement(5063..5983)
                     /locus_tag="VMZ53_06140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HUS28066.1"
                     /translation="MTPETFEDLVRPYRRELAAHCYRMLGSLADAEDQVQETLLRAWR
                     GIDGFAQRSSVKSWLYRIATNACLDVLRARPRRAMPMETTGPHDPAQPLPAANPEVLW
                     LEPAPASLVGPEATLAIRESVQLAFVTALQQLPAMQRAAVLLADVLEWQVAEISELLE
                     SSPAAINSALQRARATLGGRKRPGKPVDRALLERYMQAWERGDAAAFAALLHEDVVVS
                     MPPTPAWFQGRDAVVAFLGPRMEPGAQRMVLVDGADDVTFGFYRAEPTGTLTARAIEV
                     AVWEDGLVREIHAFFDRALVARWGVPAIVR"
     gene            complement(6027..6545)
                     /locus_tag="VMZ53_06145"
     CDS             complement(6027..6545)
                     /locus_tag="VMZ53_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28067.1"
                     /translation="MTSRSRRIAAGAVALIAAAVAIALVLNRGDSDSREVATTARSVA
                     DLNDPVVVEFRDTERSCITAFNGALARQRRNEIDELGLASAIEQEVLPPWQALRTKLD
                     AATPPPRNVALYSALRRYVSARQTGWQAYVAGLRSPEGEARRHYDAYHARDAEADAIA
                     REIGAIFRAAAP"
     gene            6635..7990
                     /locus_tag="VMZ53_06150"
     CDS             6635..7990
                     /locus_tag="VMZ53_06150"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HUS28068.1"
                     /translation="MAYRPVGEVPEAVHAIARPLRGDGGAELDQIAQSVADRRIVMIG
                     EASHGTQDFYAMRAALTRRLVEEHGFTAVCVEGDWPDCLRVDRFVRGLGDDDTADEAL
                     ASFERFPRWMWRNEDVAEFATWLRNYNLEREGRERCGFYGIDLYSLHASMQALIHYLE
                     ENDDKAAARAKERYACFDHVGVVDPQQYGLQAHIGAGRTCEGEVVAQLVEMQMRKLAR
                     SGRSPTGDAWFHAVQQAHVVRNAEAYYRTMFAGRSTSWNLRDTHMMDTLDFIADHLGD
                     GAPAKVIVWAHNSHVGDARATELGDDGQVTIGQLARQRHPKEVALIGFTTYTGSVIAA
                     HDWEDPAQRMRVRAGLPGSWEELFHEAQLPRFYVTAPDLKTHIGEGVERIQRAIGVVY
                     RPDNERRSHYLHARLADEFDVVIHLDATRGLTPLDPLVESDQAAPHFATEVPETFPTG
                     V"
     gene            7993..8349
                     /locus_tag="VMZ53_06155"
     CDS             7993..8349
                     /locus_tag="VMZ53_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28069.1"
                     /translation="MAHKSHKKHIKHLHQHEPATPKAKSPVAKAEAAQARVAKSRTAN
                     GPAKAEKAGKKAGLVRKLARTATKTVAAKPKKIISRARARVENVVKARVEGAKARVEG
                     AAGKVSARVKSLIGVA"
     gene            8771..>9099
                     /locus_tag="VMZ53_06160"
     CDS             8771..>9099
                     /locus_tag="VMZ53_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28070.1"
                     /translation="MVQLQLLGRVDTLESYRRHLTKLYGFHRPLQTVFAADSELGAMG
                     LATPTRLVPLRDDLLALGATATDLASLRGCSVVLEANNSPRLLGWFFVAERMALLSTL
                     ILRKLKRR"
BASE COUNT         1488 a         2893 c         3114 g         1604 t
ORIGIN      
        1 cgaatcgctc gcgagctgcc catcctcgtc gcgaatctct ttcgccaagc cccagtcgat
       61 cacgaccgtc tcgccgaacg gaccgatcag cacattcgac ggtttcaagt cgcgatgcag
      121 cacgctctcg ctatgcgcat acgcgatcgc atcgcacacc gcgatcgcat gcggcaacag
      181 cgccatgcgc tcggccaggg tcttgctctc cgcgatgatc gcttcgagcg atcgcccacg
      241 gacgcgctcc atcgcataga acgcgcgccc gtcgccgagc acgccagcgt catacacgcg
      301 gacgatcgac ggatgctgca gccgcgcggt gatcagcgcc tcgcgctcga agcgcggatc
      361 gatcatgccg ccgtcgcgct tcatcacttt gatgacgacg tcgcgccgca accgccggtc
      421 gcgcgcggac aagatcttgc ccatgccgcc gcgcgcgatc tcgacgccga tcgcatacgt
      481 ctggtcgtcg acgcgcggca gcgacggcaa atccgcaacc gtgtagccgc cgggcagcgt
      541 cgctgcgtgc gcgccaccgc cgccacgacc gccatcgcgg tcggtgtccc cgccctgcgc
      601 gggcagcgtc tctgaatgtg ccgcgtcggc gcccgccgtc gagcctgcag cggttgccgc
      661 aaatgccgcg gcgttcgcgc cgactttcgc gccgactgcc gccgcggcct tcgcgccgat
      721 tgccgcagcg ttcgcgccga ctgccgccac cgtcgagcca gcgagcgtcg ctgcgaatgc
      781 gggcgatggc gctgccgcgg gacgtgtcgg agcagcggcg ggatgtgtcg gtgcgccggc
      841 gggatgtgcc ggtgctgcgg cgggatgtgc cgctgcagcg gctggatgtg cggctggcga
      901 tggaccggct gctacgccgg gtagcgtgcc gtcggtcgca atattggcgg caggtttcgc
      961 aaccggagca atggtttcgt cggttccgat cggcgctgct gcaggcgtca ctccgggtat
     1021 cgctgcgggc atcccagcgg gcggtgcgag cggtgcgggc ggtgcgggcg acgcgggcgc
     1081 tgctgccggc gtcgctgcgg gcgctgcggg cgcacctggc cctggcagcg ttccaccagc
     1141 tgcgtcccca tcagccattg cgacgagcgt atcaccttgt cgcttccgta gatctcgtga
     1201 gacctggagc tgattgcttc agggcgcgcg acggagcttc ttgctcggcg gcagcaccaa
     1261 gtcccagaag ccgaagaccg acgcgagaat atggtcgagc tcgcgcgtct cgcggctgcg
     1321 gccgagcgtc gtcgcatcgg gcaagcagta cgccgcgagc ctcgacgctt cgagcgaatg
     1381 catcacgccc ttcgaccagc cgctgcccgc gtacacgagc aagcccggca tccaaagcgc
     1441 ctcgaggtcg gcgagcgcgt acggaatctt ttcgtcgacg gtgccctgcg agtcttggta
     1501 cttgcactcg atcgcgatcg ccttacggtc ttcgggccgc acaacgaaga tatcgatgaa
     1561 gcgatccttg ccgatgatcg tcttcccgag cgaaatctcg agatagacgt cgaggccgtg
     1621 cggcgcgtag ttctcgtgga tgtagctcgc gatgagatcg cgatagccgt tcccggtcat
     1681 cgcggtgagc gtctgcttgc tgaggttcgg cgcggcgccc gccttcagcg ctgcaatcgc
     1741 gcccttcggc tgcgcgagca tccggctcgc gatgagcttc gaccgagacg gcggtttcgt
     1801 cggcgtcgcc atcgcgctag ctcctcgcct cgacgaacga atcacgggac ccacgtgggc
     1861 tcgtcatcgc gtcggtgttc ggctcggcgt acccacgtgg gtttgtcagc gcgtcggtgt
     1921 ttggctcggc gcgcgtcatt agatcgtcag cccgagctgg ggttgcgacg tcacgcgaag
     1981 tggcagctcg ccttcggcgc caccggcttc gaggaggcgc tgttgcgtga tctcgagtgc
     2041 ttcggcgcag agatcgttgc cgcggaagtt gcgcttgttg cggatcgagg cgaggccgac
     2101 ggagcccgag cccatgaacg gatcgatgac gagatcgccc gcgtccgtgc tctgcttgat
     2161 caggatctcg ctgacctcgg gcggcttctc ggcgggatag ccgttgttga tgcgcggcgc
     2221 ctcgatgatg tcggcgatgc cgaggtcgag cagcttgcgc ttgcccttct cgaagaagag
     2281 cacgcactcg tagcgagcac ggtagtgata tcccataccg atccggactt tgtcccagat
     2341 gagcggcttc cagaacctga agcccgcttc ttccgcgagc ggcttcgcga cgaacatcgt
     2401 ctcgggatcg cagaagagat agaagtgcgt gtccttcttc agcacgcgga acacttccgc
     2461 gaacagctcg gggaagcggg cgtttggaaa gatcgagaac caatcgttgc tcgatgcttt
     2521 gctgtgcttg aggcgcgtcg ttgtaccgat cgcgcggtgt ttttcgagcg actcgtacgg
     2581 cggatccgtg atgactagat cgacggagct cgccggtagc gtccgcagcc aggcgacggc
     2641 gtcgccttgc gtgagctgga acctcggctg atccatgcgc tgaccctcgc atggaggtct
     2701 gacaatctgt tcagtagcgc gtgttcagtg cggtgatttc gtgcgtgttc tcgacggaag
     2761 atctcggcga tgctgcgggc gcatggcgag cgacgcagac ggcgacgacg acttcggcgg
     2821 gctcgctcgc gatctcgaga cgatgtcgcg gcggcgcgtg ctcgagctcg tcacgaaggc
     2881 ggcggccggg atcacgctcg caccgatcat cggagcgtgc ggcgacaacg gagcggacgt
     2941 catgcgcgac gcgaacgcgg taggcgatag cgcgagcgat cagtgcgcgc gcattccggg
     3001 agagacgccg gggccttatc cgggcgatgg cacgaacggc cccaatgcgc tgacgatcgc
     3061 gggcataaat cgcagtgaca ttcgcacgag catcggatcg gcgagcggga cggcgaccgg
     3121 catcgagctc gccgttacgc tcacggtcgt caatgccgcg acctgcgagc cgctcgtcgg
     3181 ctacttcgtc tatctgtggc attgcacgca ggccggcgac tactcgatgt acacgggcgc
     3241 ggcgcagagc gagaactacc tgcgcggcgt gcaggcgacc gacagcgccg gcaaggtcac
     3301 cttcacgacg atctttcccg gctgctatcc gggacggtgg ccgcacattc acgtcgagat
     3361 cctcagcggg tccatgacga aggttgcggt ctcgcagctc gcgctgcccg acgcgacgtg
     3421 cgacgcggtg tacgcgacgg cgggctacga agcgagcgcg acgacctttg cgcagacctc
     3481 gctcgcgaac gacagcgtct ttcgcgacgg ctcgtcgcgg caagtggtca gcgttaccgg
     3541 cgcggtcggc agcaacctgg cgggcgcgct cacgatcgcg atcgcggtct aggtctcggt
     3601 cgcgagctcg taggagatcg tcacgtcgag cgtgagcggc tggagcgtgc cgccgagcgc
     3661 gacgcccgaa ttgcggacgt cgacggagtt cgcgacttgc gggaagtacg tggcgctcca
     3721 catgtagcca ccttggttct cggcgactgc cgtgatgcgc ccgagcttga tcccgagggt
     3781 cttcgcggtt tgctcggcct tctccttcgc ggctttgagc gcatcttcgc gaacctgctt
     3841 cttgagcgag gccatgtcgc tgcgccggaa ctgcgtcgac atcgagctcg cacccgcttg
     3901 gccggcggag tcgagaatgt cggcgatctt ggagaagtcg cgcgtcgtcg cggtcacggt
     3961 gatctgcgcg cggtacgtat ggatcttgag tgttgcccag ccttctttgc tttgctcgta
     4021 gaccggatcg agcgacagcg tagagagctt gacgtcgttc gtctcgacgc cggctttcag
     4081 gagcgcggcg atgaggagct gcttcttcgt ctcgagatct ttcgtcgccg tgcctgggcg
     4141 catcgagtcg gcggtgatcg tgatcgtgag gtctgcgcag tccgggctga cctccagcgt
     4201 ggcttgcccg ttgacggtca tcccgcccgg ctgtttcgat ggcgccttcg gatcgggcgc
     4261 ctggacggcg atgacttgcg ggcgatcgtt gcaggcggcg acggtcgtaa gcgaggtggc
     4321 gagggcgagt gcggcgaggg tcttcttcag catgtgcggc tccttcgctt ctggagacgc
     4381 atctcgcggg cgttggtatg cctcccgagc gagggcctta cacacctctt acaagccgct
     4441 tacgccgact tttcgaggtg cggcgccacc tgctcgaaca cgctccggac gctcggcctc
     4501 tcgagcgcgc gagacatgta ggcaacgacg tgcttatggc gatcgaacgg atggagctcg
     4561 cgcgcgtagc gcagggccgg gatgaacgag cagtcggcca tcgtgagctc gtcgcccatg
     4621 atccagggcc gcttgctgat gtgctcgtcg aggcccgcca gcatcgcgtc gaggcgggtg
     4681 tgcgctgcat cggcgtcacc gcgcttgaag agcagcgtca tcagcggctc tgtgacgtag
     4741 aggtccgcga tgcgatcgta aaatcgcgtc tggcgcgcga ggtctcgatc gtcggggatg
     4801 agccgcgtcc cggtcgagaa gtgcgtgtcg agatactcga tgatgatcgt cgactctgga
     4861 atcttccagc cgtcggcgag cacgaggagc ggcaccttct tgatcggcgt cagcttcgcg
     4921 aactgcgcgt catcggggcg gatcatgatc ggctcgtacg cgacgcgctt ctcggcaagc
     4981 gcgatcatga ctttgtgaga gtagggagaa cgggggtggt agtagagctg catgccgtaa
     5041 agacgatcac ggccgccgaa actcatcgga cgattgcagg gactccccag cgcgccacga
     5101 gagcgcggtc gaagaatgca tgaatctcgc ggaccaagcc gtcctcccac accgcgactt
     5161 cgatcgcccg cgccgtaagg gttcccgtcg gttccgcacg ataaaagccg aacgtgacgt
     5221 cgtctgcgcc gtcgacgagc accatccgct gcgcgccggg ctccatgcgc gggccgagaa
     5281 atgcgacgac ggcgtcgcgt ccctgaaacc acgctggggt cggcggcatg ctgaccacga
     5341 catcttcgtg cagcagcgcc gcgaaggccg ccgcatcgcc gcgttcccac gcctgcatgt
     5401 agcgctcgag caatgcgcgg tcgacgggct tgcccggccg cttgcgtccg ccgagggtgg
     5461 cgcgtgcgcg ttgtagcgcg ctgttgatcg cagcgggcga cgactccaac agctcggaga
     5521 tctcggcgac ctgccattcg agaacgtcgg cgagcagaac ggccgcgcgc tgcatcgcgg
     5581 gcaactgctg cagcgccgtc acgaacgcga gttgcacgct ttcgcggatc gcgagcgtgg
     5641 cctccggacc cacgagcgac gcgggcgcag gctcgagcca cagcacctcg ggattggccg
     5701 ccggaagtgg ttgcgccggg tcgtgtgggc cggtcgtctc catcggcatc gcacgccgcg
     5761 gacgcgcgcg caggacgtcg aggcacgcgt tcgtcgcgat ccgatagagc cacgacttca
     5821 cggacgaacg ttgcgcgaag ccgtcgatcc cacgccacgc gcgcagcagc gtctcttgca
     5881 cctggtcctc ggcgtcggcg agcgagccga gcatgcggta gcaatgagcc gcgagctcac
     5941 ggcggtacgg gcggacgagg tcttcgaagg tttcgggagt catagctgcc taagagacga
     6001 gtgggcggtc gccgaatcat cggtctttat ggcgccgccg cgcgaaagat cgcgccgatc
     6061 tcgcgagcga tggcgtcggc ctcggcatca cgtgcgtgat aggcatcgta gtgacggcga
     6121 gcttctccct cgggactgcg caagcccgcg acatacgcct gccagccagt ctggcgcgcg
     6181 gagacataac gccgcagcgc ggagtacagc gcgacgttgc gcggcggcgg cgtggccgca
     6241 tcgagcttgg tgcgcagcgc ttgccacggc ggcagcacct cttgctcgat cgcacttgcg
     6301 aggccgagct cgtcgatctc gttgcggcgc tgacgcgcga gggcgccatt gaaggcggtg
     6361 atgcaactgc gctcggtgtc gcgaaactcg acgacgacgg gatcgtttag atcggcaacg
     6421 ctgcgcgctg ttgtcgccac ctcgcgcgaa tccgaatcgc cccgattcag cacgagcgcg
     6481 atcgcgacag ccgcggcgat gagcgcgacg gcaccagctg cgatgcgacg agagcgcgag
     6541 gtcacgatga cgttctatca ccgcgcaacg gcgcgcagcg tgcgatgcaa ggcgcacatt
     6601 tgacaggcgt tttgcgcgtt gcagggtagg actaatggcg tatcgccccg ttggagaggt
     6661 tccggaagca gtgcacgcga tcgcgcgtcc gctgcgaggg gacggtggcg ccgagctcga
     6721 tcagatcgcg cagagtgttg ccgaccgtcg catcgtgatg atcggcgagg catcgcacgg
     6781 cacgcaggac ttctatgcga tgcgcgcggc gctgacgcgt cggctcgtcg aggagcatgg
     6841 cttcaccgcc gtgtgtgtcg aaggcgattg gccggattgc ttgcgcgtcg atcgcttcgt
     6901 gcgcgggctc ggtgacgatg acaccgccga cgaggcactc gcatcgttcg agcggtttcc
     6961 gcggtggatg tggcgcaacg aagatgtcgc ggagtttgcg acgtggctgc gcaactacaa
     7021 cctcgagcgc gagggtcgcg agcgctgcgg cttctatggg atcgacctgt actcgctgca
     7081 cgcctcgatg caggcgctga ttcactatct cgaggagaac gacgacaagg ccgctgcgcg
     7141 cgccaaagag cgctacgcct gcttcgatca tgtcggcgtc gtcgatccgc agcagtacgg
     7201 attgcaggcg catatcggcg cgggccgcac gtgcgaaggc gaggtcgtcg cgcagctcgt
     7261 cgagatgcag atgcgaaagc tcgcgcgctc ggggcgttcg ccgacgggcg atgcgtggtt
     7321 ccacgccgtt cagcaggcgc atgtcgtgcg caacgccgag gcgtactacc gaacgatgtt
     7381 cgcgggccgc agcacgtcgt ggaatctgcg cgacacgcac atgatggata ccctcgactt
     7441 catcgcggat catctcggcg acggcgcgcc ggcgaaggtc atcgtgtggg cgcacaactc
     7501 gcacgtcggc gacgcccgcg cgacggagct cggcgacgac ggtcaggtga cgatcggtca
     7561 gttggccaga cagcggcatc ccaaggaggt cgcgctgatc ggcttcacga cgtacacggg
     7621 atctgtgatc gctgcgcacg actgggaaga tcccgcgcag cgaatgcgcg tgcgcgctgg
     7681 cctgccggga agctgggaag agctgtttca cgaagcgcag ctcccccggt tctacgtcac
     7741 ggcgccggat ctgaagacgc acatcggcga gggtgtcgaa cgcatccagc gcgcgattgg
     7801 cgtcgtgtat cggccggata acgaacgtcg cagccactat ctgcacgccc ggctcgccga
     7861 cgagttcgat gtggtgattc acctcgatgc gacacgaggg ctcacgccac tcgatcctct
     7921 cgtcgagagc gatcaggctg ccccgcattt tgcaactgaa gtcccggaaa cgttccctac
     7981 cggagtttga cgatggccca caagtctcac aagaaacaca tcaagcacct gcaccagcac
     8041 gagccggcga cgccgaaggc gaagtcgccc gtcgcgaagg cggaagcggc acaagcgcgc
     8101 gtcgcgaaga gccgcacggc gaacggcccg gcgaaggccg agaaggccgg caagaaggcg
     8161 gggctcgtcc gcaagctcgc tcgcacggcg acgaagacgg tcgcggcgaa gccgaagaag
     8221 atcatctcgc gtgcgagggc tcgcgttgaa aacgtcgtga aggcccgcgt cgaaggcgcc
     8281 aaggctcgcg tcgaaggcgc ggccggcaag gtcagcgcgc gcgtgaagtc gctgatcggc
     8341 gtggcgtagc cggctcctag cccgcccgcg agagcgatgt gctcgaccta cttcaatggc
     8401 cggcgatggc cattaccgtc atcgcggcgt ggctcgtcgc atcgcgaagt gcgcgcaagc
     8461 gctcggtcgg gttttggttc ttcatcgcga gcaacgttgc ttgggttgca tggggcatcc
     8521 atgcgcacgc ctgggcgttg atcgtcctgc aagtcgcact cgcggcgttg aacatccggg
     8581 gagttcgaaa aaacgacccg tgacgtagcc gcacgtcgtg gcgtttgcgg tcatttcgag
     8641 caacgcatgc gagcgtcatg catcgaaatg cgggcatcgt ccttgctgtt acgcctcaga
     8701 ctacatgctg tcgtcttgga cgttacggga gttgctgcga cgaacgaagt cgcaccatgc
     8761 cgatgctgat ttggttcagc tgcaactgct cggacgcgtg gacacgctcg agtcgtatcg
     8821 gcggcacctc acgaagctgt acggcttcca tcgcccgctg cagaccgtgt tcgccgcgga
     8881 ctccgagctc ggcgccatgg gtctcgccac accgacccgg ctcgtaccgc tgcgcgacga
     8941 tttgctcgcg ctcggtgcaa cggcgacaga tctcgcgtcg ctccgcgggt gttcggtcgt
     9001 actcgaagcg aacaactcgc cgcgtctcct cggatggttc ttcgtcgccg agaggatggc
     9061 cctgctcagc acgctcatcc tgcgcaagtt gaagcggcg
//
LOCUS       DATLBZ010000116        11453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2349334, whole genome shotgun sequence.
ACCESSION   DATLBZ010000116 DATLBZ010000000
VERSION     DATLBZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2349334"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VMZ53_06165"
     CDS             <1..741
                     /locus_tag="VMZ53_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HUS28071.1"
                     /translation="FCAYVQKHLLDEVHYVDIVDCNAGSHGKAFATNFNPEINIREIT
                     ARGRYWENGEWKETDPLSVSKIFDYPGVGEKKSFLLYHEELESLAKNLKGLKRIRFWM
                     TFGEAYLKHLEVLQNVGMTRIDEIEFEGKKIVPIKLLRALLPDPSSLAAGYSGKTSIG
                     CLCEGIKNGEKKRYFIYNICDHAETYKEVRAQAISYTTGVPAVTGAVMMVTKQWKGDG
                     VFNMEQMDPDPFLADVAKRGLPWHVEER"
     gene            751..1926
                     /gene="nspC"
                     /locus_tag="VMZ53_06170"
     CDS             751..1926
                     /gene="nspC"
                     /locus_tag="VMZ53_06170"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HUS28072.1"
                     /translation="MSKGASLGEDVARAIDITNVETPVFITDMGALERNLQTLVDVQK
                     RAGCTILLALKGFAQWSTFPLVRRYLAGATSSSVAEARLAREELGGEVHAYAPAYSDG
                     EMRELVTLADHVVLNSPAQWKRHRAALERAGISAGMRVNHEHQEAEVALYDPAGACSR
                     LGTTRTNLDPAHGDLDGIDGLHFHSLCQNNSDALERAVAAFEQKFGDLIPRMKWINFG
                     GGHHITRPDYDIAKLVDVVTKFRTKWQKKVYLEPGEAIALGTGVLVASVMDTFHNGMD
                     IAILDTSATAHMPDVLEMPYRPVIVDGADPGVKKHTYRLGGMTCLAGDVIGDYSFDRP
                     LQIGDKLVFLDMAHYTMVKTTTFNGVRMPSIATHDPATKQITVHRRFGYTDYRDRLS"
     gene            1923..2831
                     /locus_tag="VMZ53_06175"
     CDS             1923..2831
                     /locus_tag="VMZ53_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HUS28073.1"
                     /translation="MKYEKGTRNDDVVDVRGRKSSAAPKIAGGSIVVVLLLFVASRFL
                     GVDVSNIGGGGGGGGGGDDPSAGGQLSSAEHEAQKGSAAPLDGPDPDAELVDFISFAM
                     KDIQDTFEQLYQESNTPYHRAKLYIFNEAINTACGRSTKAIGPFYCPGDWNAYIDLSF
                     YKELRARFGAPGDFAQAYVLAHEIGHHLQNLAREQGREPKCPRGESRNQCSVHVELQA
                     DCFAGVWGHHAQNKKLLDAGDLEEAITAATAIGDDRLQKQAGADDVNPESFTHGTSEQ
                     RVAWFRRGFDSGRLDACNTYAAAMPR"
     gene            3002..3517
                     /locus_tag="VMZ53_06180"
     CDS             3002..3517
                     /locus_tag="VMZ53_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28074.1"
                     /translation="MRSTSAAVLLALVACGTELPSGDSRDPVNTAPDAGVGSQSSDGG
                     VTGAPGDGSTTPLTDCQEATQHSDLAWIQQKVFTPSCTDECHSTPDPSSGMDLTAGHA
                     YTNLVNVNSNQFSGWKRVVPGDPRSSMLMVQIGGEQGPALEGTMPWGQPKLCDEKIDA
                     IRRWIAAGAPQ"
     gene            complement(3536..4102)
                     /locus_tag="VMZ53_06185"
     CDS             complement(3536..4102)
                     /locus_tag="VMZ53_06185"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HUS28075.1"
                     /translation="MRAALSAIVLALLASSASAEAPHTVRGETIHVEGKAPPGTPPKP
                     AKKYHAIAPRYSDYAIEHDTWARAWLLLDIDARGVVTRVKLVRKPGADLDQIAIDTAM
                     KMRFEPSRDALGAPQPSRLLWTIEWPSWGWLIAHEGLATKIPASVNGVPCADSGQPLN
                     LDAVHPVYRDCTLPDVSKIDSLPWTAPK"
     gene            complement(4148..4747)
                     /locus_tag="VMZ53_06190"
     CDS             complement(4148..4747)
                     /locus_tag="VMZ53_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28076.1"
                     /translation="MRRALLLAVTLASLPAAADPPPRYVPGETIVIEGKAPIPKVAPH
                     PKKNYHRMAPPYSDEAILKDAWSKAWVLLDIDAHGVVKRVKLLKRPGYGLDEIAIAHA
                     MKMRFDPAEDAQGYAIPTQLLWSIEWPSYWWLVDLDIPPTTVPDFVNDPDHFKTAPGF
                     TVTCKGDGPLQMGSLHPTNRDCSGPPPVSDFNKVAWVTK"
     gene            complement(4887..6185)
                     /locus_tag="VMZ53_06195"
     CDS             complement(4887..6185)
                     /locus_tag="VMZ53_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS28077.1"
                     /translation="MSARFDVDFFVIGGGSGGVRAARVAAEHGARVAIAEESRWGGTC
                     VVRGCVPKKLMVYASEVSRTIDDARGQGWTISDVSFDWPTFLALKDQEIARLSAAYRT
                     RLEKARVRVIDGRARLVDAHTIEVAGERVTAANILVATGGRPRVPADHPPGGTHRYVT
                     SDEVFHLPELPKRIGILGAGYIGIEFAHIFRGFGCDVTLVHRGEHVLRGFDPELAREV
                     EAGIARHGIKLVTGDHIDFDVDLAMAAIGRDPLTAGIGLVEAGIVLDGRGAVVVDEWS
                     RTNVPHIYAVGDATARVQLTPIAIREGHAVADTVFGGKPTPIKHEHIPTAVFCQPPLA
                     TVGLGEPAAIAAGHDVQIFKARFKPMRYALSKRDEHVLIKVVVSRANDAVLGVHMVGP
                     DAPEIVQTAAIALTMGAKKADLDRTFALHPTTAEELVLLR"
     gene            6315..7337
                     /locus_tag="VMZ53_06200"
     CDS             6315..7337
                     /locus_tag="VMZ53_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28078.1"
                     /translation="MMRGITMRSSLTLGSVALLALLAACGTDPAIGDDDDGTGSGSGS
                     GSGSGSGMDPEPPARGFQMKSPEITIKAGQEITYCWYFKTPNTELFTIKRWVSHMTGG
                     SHHMIVFFTNSLSQPEGTVSSSNCGFGGGTNVGQWIYAAQNEDADLPLPSDDGAGKPV
                     GMDVKPAQTGFIQMHYLNTADQDITVHVTLNGEAYEAGVDATKTFAYVTFAGDIDVLP
                     MAQGYKNTHTCPIPATSKVWLMSTHAHARAAHTEVRDGAATSTNVVFASDDWEHPGAK
                     AWMTNPFYTFASGKLTYECTYNNPSSNEYKTGDSAQTNEMCMATGYYFPATKATICYN
                     SNILPF"
     gene            complement(7350..7664)
                     /locus_tag="VMZ53_06205"
     CDS             complement(7350..7664)
                     /locus_tag="VMZ53_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28079.1"
                     /translation="MRRLLLLLALSACSHAKATGPAWPEMAKTEPEDDGGESIAPHES
                     STVAAAIEKSDEAETKKDDAPAAAVTEQVDEEKPAASSAPTQPANDDVIMSEEIIIEI
                     DD"
     gene            complement(7683..8180)
                     /locus_tag="VMZ53_06210"
     CDS             complement(7683..8180)
                     /locus_tag="VMZ53_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HUS28080.1"
                     /translation="MRLLAFVGIVWMAAACQKADSVKASAADTQHARPAPGAIAPSPD
                     PDRTMPAPTAEKTTAAPSSCGGSCGGGGSCSGACGDSCGGAGAMPQWAAIGGDATWQP
                     VYVEGMKCGGCAKRIEKALANVDGVLAVKTDVRTKKIEIVTRQGLDAKSLVKPTIDGL
                     GYRVQ"
     gene            8204..8674
                     /gene="bcp"
                     /locus_tag="VMZ53_06215"
     CDS             8204..8674
                     /gene="bcp"
                     /locus_tag="VMZ53_06215"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HUS28081.1"
                     /translation="MVEVGKKAPAFALESSDGGTVKLKELEGKIVVLYFYPRDNTPGC
                     TVQAEGFRDAIPALKKLGAVVLGVSKDTIASHCKFRDKYKLNFPLLTDADGATMEAYG
                     AWGDKVMYGKKMKGIIRSTVLIDKTGKVAQHWPKVSVKGHVDEVVDAVKALTKK"
     gene            complement(8678..>11453)
                     /locus_tag="VMZ53_06220"
     CDS             complement(8678..>11453)
                     /locus_tag="VMZ53_06220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HUS28082.1"
                     /translation="VCERAQGLIALWGGDTSLLAGELDPPPDVVADLQHAFGDRLYAI
                     LSRHRRADDVPREARLRARAAAHRIPLVAATEVLYHSRSRRQLQDVLTCIRHGVTLAT
                     AGRLIRGNDEHDLRPPHAFKKLYADEPGAFARTLEIAARCTFSLGELRYRYPSERLPD
                     GSTSAAYLRKLAFEGAAWRYDNDIPPNVRAQLDAELSVIEDLDYPGYFLTMYEIVSYC
                     RRRDIMCQGRGSAANSAVCFCLGITAVDPVRMGLLFERFLSRERAEPPDIDLDIEHER
                     REEVIQHVYSVYGRDHAAMVCNIIRYRPRSAVRDVGKALGIPETALDRAAKHLSMYGT
                     VEHEALARSGLAEGGHASALDHLARLSEEILEFPRHIGIHPGGFLLGHEPVHDIVPIE
                     NATMPGRTVIQWDKDDLEDLGLFKVDLLGLGALHQLHLGLDMLRHHRGIDLSMATIPA
                     EDAATYDMICTADTVGTFQIESRAQMSMLPRLKPRTFYDLVVEVSLVRPGPISGGMVH
                     PYLRRRKGEEKVEYPHPCLESVLAKTLGVPLFQEQVMRLAVVAGDYTPGEADQLRRDM
                     AAWRRSGRIEKHRERLISRMEAKGIAREFSERVFEQIRGFGEYGFPESHAASFALIAY
                     ATCWMRKHYLPEFTASLLNAQPMGFYSPSTIVGDAQRHGLTILPIDVTCSEWNCTLEP
                     LPNDDVFQFAVRMGLRWIKGIQIAEGAKITAARRERAFDSVEDFVRRTHLPVRTHSQI
                     AECGALGPLVRERRDALWQVTGWVRRQDDPLDLGGDVHDVQFGKLTKMDEIFWDYRSS
                     DHSTRGHPLEPMRGELRAKRLPDARTVSRGRDGQRIDYVGIVICRQQPGTASGVTFMT
                     MEDETGFVNLVVWAQVFDKYSTIIRTTSLLGVSGRLQVQEGIVHLVAEQLWHPDLSRE
                     VVEVDSRDFH"
BASE COUNT         2062 a         3727 c         3710 g         1954 t
ORIGIN      
        1 ttctgcgcgt acgtgcagaa gcacctgctc gacgaggtgc actacgtcga catcgtcgac
       61 tgcaacgcgg gctcgcacgg caaggcgttc gcgacgaact tcaatcccga gatcaacatc
      121 cgcgagatca ccgcgcgcgg ccgctactgg gagaacggcg agtggaagga gaccgatccg
      181 ctcagcgtgt cgaaaatctt cgactacccg ggcgtcggcg agaagaagag cttcctcctc
      241 tatcacgagg agctcgagtc tctcgcgaag aacttgaagg gcctcaagcg catccgcttc
      301 tggatgacgt ttggcgaggc gtacctaaag cacctcgagg tgctgcagaa cgtcggcatg
      361 acgcgcatcg acgagatcga gttcgagggc aagaagatcg tcccgatcaa gctgctccgc
      421 gcgctgctgc cggatccgtc gtcgctggcc gccggctact cgggcaagac gtcgatcggc
      481 tgcctctgcg aaggcatcaa gaacggcgag aagaagcgct acttcatcta caacatctgc
      541 gatcacgcag agacgtacaa agaagtccgc gcgcaggcca tctcgtacac gacaggcgtg
      601 ccggcagtga ccggcgccgt gatgatggtg acgaagcagt ggaagggcga cggcgtcttc
      661 aacatggaac agatggatcc cgatccgttc ctcgctgacg tcgcgaagcg cggcctgccc
      721 tggcacgtcg aagagcgctg agcctcggcg atgtcaaaag gcgcctcgct cggcgaagat
      781 gtagctcgcg cgatcgatat tacgaatgtc gagacgccgg tgttcatcac cgacatgggc
      841 gcgctcgagc gaaatctcca gacgctcgtc gacgtgcaga agcgcgcagg ctgcacgatc
      901 ctgctcgcgc tcaaaggctt cgcgcagtgg tcgacgtttc cgctcgtgcg gcgttatctc
      961 gcaggtgcaa cgagcagctc ggtcgccgag gcgcgactcg cgcgcgagga gctcggcggt
     1021 gaggtccacg cgtatgcgcc cgcatattcc gacggcgaga tgcgcgagct cgtcacgctc
     1081 gccgatcacg tcgtcctgaa ctcgcctgcg cagtggaagc ggcaccgcgc ggcgctcgag
     1141 cgcgccggga tctcggccgg catgcgcgtg aaccacgagc atcaagaagc cgaggtcgcg
     1201 ctctacgatc ccgccggcgc ctgctctcgc ctcggcacga cgcgcaccaa cctcgatcca
     1261 gcgcacggcg acctcgatgg tatcgacggc ctccacttcc actcgctgtg ccagaacaac
     1321 agcgatgcgc tcgagcgtgc agtcgccgcg ttcgagcaga agttcggcga tctcatcccg
     1381 cgcatgaagt ggatcaactt cggcggcggt catcacatta cgcggcccga ctacgacatc
     1441 gcgaagctcg tcgacgtcgt cacgaagttc cgcacgaagt ggcagaagaa ggtctatctc
     1501 gagcccggcg aagcgatcgc gctcggcacg ggcgtcctcg tcgcgagcgt gatggacacg
     1561 ttccacaacg gcatggacat cgcgatcctc gatacgtccg ccacggcgca catgccggac
     1621 gtgctcgaga tgccgtaccg cccggtgatc gtcgacggcg cggaccccgg cgtgaagaag
     1681 cacacctatc gcctcggtgg catgacgtgc ctcgccggcg acgtcatcgg cgactacagc
     1741 ttcgatcgcc cgctgcagat cggcgacaag ctcgtcttcc tcgatatggc gcactacacg
     1801 atggtcaaga cgaccacgtt caatggcgtg cgcatgccgt cgattgcgac gcacgatccg
     1861 gcgacgaagc agatcacggt gcaccgccgc ttcggctaca ccgattaccg cgaccggctg
     1921 tcatgaagta cgagaagggc acgcgcaacg atgacgtcgt cgacgtccgc gggcgcaagt
     1981 cgtcggcggc gccgaagatc gcgggcggat cgatcgtcgt tgttctgctg ctgttcgtcg
     2041 cgtcgcgctt cctcggcgtc gacgtctcga atatcggcgg cggaggagga ggcggaggag
     2101 gcggcgacga tccgagcgcg ggcggtcagc tcagcagcgc cgagcacgaa gcacagaagg
     2161 gctcggctgc gccgctcgac ggtcccgatc ccgacgcgga gctcgtcgac ttcatcagct
     2221 ttgcgatgaa ggacatccaa gacacgttcg agcagctcta ccaagagagc aacacgccgt
     2281 atcaccgcgc gaagctctac atcttcaacg aagcgatcaa caccgcgtgc ggtcgctcga
     2341 cgaaggcgat cggcccgttc tattgccccg gcgactggaa cgcgtacatc gatctgtcgt
     2401 tctacaaaga gctgcgcgcc cggttcggcg cacccggcga cttcgcgcag gcgtacgtgc
     2461 tcgcgcacga gatcggccac cacctgcaga acctcgcgcg cgaacagggt cgcgagccga
     2521 agtgcccgcg cggcgaatcg cgcaatcagt gctcggtcca cgtcgagctg caagccgact
     2581 gcttcgccgg cgtgtggggc caccatgcgc agaacaagaa gctcctcgac gcgggcgatc
     2641 tcgaagaagc gatcacggcc gccacggcga tcggcgatga ccgcttgcaa aagcaagccg
     2701 gggccgacga cgtgaatccc gagtcgttca cgcatggcac gtccgagcag cgcgtcgcgt
     2761 ggttccgccg cggattcgac agcggccgcc tcgacgcgtg caacacgtac gcagccgcaa
     2821 tgccgcgcta aggcgtgtcg tcggcgcacg ccggttttcg aaacagtacg tttccatcca
     2881 tccgcggtgt gcggagaagc ccgcagctgc gagcgcgcgc tatctgccgg cgcgctgaat
     2941 gcgctaaccc acgaaatcgc gagcgtcgtc ggtggtgcga agcttgctga atgtccaccg
     3001 catgcgttcg acctccgctg ctgtgctgct cgcgctcgtc gcgtgcggta ccgagctccc
     3061 ctctggtgac tcacgcgatc ccgtcaacac ggcgcccgat gccggcgtcg gctcacaatc
     3121 ttctgacggt ggcgtcaccg gtgcacctgg cgatggcagc acgacaccgc tgaccgactg
     3181 ccaagaagcc acgcagcatt cggacctcgc gtggatccag cagaaggtct tcaccccgtc
     3241 ttgcaccgac gagtgccaca gcacgcccga cccgagctcc ggcatggatc tcacggcggg
     3301 ccacgcgtac acgaacctcg tcaacgtcaa ttcgaaccag ttctccggct ggaagcgcgt
     3361 cgtccctggc gatccgcgct cgagcatgct gatggtccag atcggcggcg agcaaggccc
     3421 ggccctcgaa ggcacgatgc cgtggggtca gcccaagctg tgcgacgaga agatcgacgc
     3481 gattcgccgc tggatcgccg ccggcgctcc gcagtagggg tccgacccca ctgctctatt
     3541 ttggagcagt ccaaggcagc gagtcgatct tcgagacatc gggcagcgtg cagtcgcgat
     3601 agaccggatg cacggcgtcg aggttgagcg gctggccgct gtccgcgcac ggcactccat
     3661 tgacgctcgc cggaatcttc gtcgcgagac cttcgtgtgc gatcagccag ccccacgacg
     3721 gccactcgat cgtccaaaga agtcgcgacg gctgcggcgc accgagtgcg tcgcgcgatg
     3781 gctcgaaccg catcttcatc gcggtatcga tcgcgatctg atcgaggtcg gcgcccggct
     3841 tgcgcacgag cttcacgcgc gtgacgacgc cacgtgcatc gatgtcgagg agcagccacg
     3901 cgcgcgccca cgtgtcgtgc tcgatcgcgt aatcggaata acgcggcgcg atcgcgtgat
     3961 acttctttgc gggcttcggc ggcgtgcctg gaggcgcttt gccttcgacg tggatcgtct
     4021 cgccgcggac ggtgtgcggc gcctccgccg acgccgacga tgcgagcagc gcgagcacga
     4081 tggcagacag cgcagccctc attcgtcgag catgcccttc ggcttcaaag gagttgttac
     4141 gcgtccgtta cttggtgacc caggcgacct tgttgaagtc cgacacgggc ggtggccccg
     4201 aacaatcgcg attggtcggg tgcagcgagc ccatctgcag cgggccgtcg cctttgcacg
     4261 tcacggtgaa gccgggggca gtcttgaaat ggtctgggtc gttgacgaag tccggcaccg
     4321 ttgtcggcgg gatgtcgaga tcgacgagcc accaatacga cggccactcg atcgaccaca
     4381 gtagctgcgt cgggatcgcg tagccctgcg catcttcggc ggggtcgaag cgcatcttca
     4441 tcgcatgcgc gattgcgatc tcgtcgagac cgtaccccgg acgcttcaac agcttcacgc
     4501 gcttcaccac gccgtgggcg tcgatatcga ggagcaccca cgctttcgac caggcgtcct
     4561 tcaagatcgc ttcgtcggag tacggcggcg ccatgcggtg gtagttcttc ttcgggtgtg
     4621 gcgcgacctt cggaatgggc gccttgcctt cgatgacgat ggtctcgccc ggcacgtagc
     4681 gcggaggcgg atccgcggca gcaggcagag acgcgagggt gactgcgagc agaagtgcgc
     4741 gacgcatgtc tgccgggttt tgcacgtcgt gcaccaacgc cacgttgcgc agaatcgcga
     4801 gtctggcggg ttttgcgcga tgtgcccgta tcccgcgcac cacgcgacac cactaacaac
     4861 tcacaccacg caggtgtcag gccgcgtcag cggaggagca cgagctcttc ggccgtcgtc
     4921 gggtgcagag cgaacgtgcg atcgagatcg gctttcttcg cgcccatcgt cagcgcgatg
     4981 gccgccgttt ggacgatttc tggcgcgtct ggccccacca tgtgtacgcc gagcaccgca
     5041 tcgttcgcgc gcgacacgac gaccttgatg agcacgtgct cgtcgcgttt ggagagcgcg
     5101 taccgcatcg gcttgaagcg cgccttgaag atctggacgt cgtggcccgc tgcgatcgct
     5161 gcaggctcgc cgaggccgac ggtcgccaga ggcggctggc agaacacagc tgtcgggatg
     5221 tgctcgtgct tgatcggcgt cggctttccg ccgaacaccg tatcggccac cgcatgcccc
     5281 tcgcggatcg caatgggtgt gagctgcacg cgcgcggtgg catcgccgac ggcatagatg
     5341 tgcggcacgt tcgtccgcga ccactcgtcg acaaccaccg cccctcgccc atcgaggacg
     5401 atgcccgctt cgacgagccc gatgcccgcg gtgagcggat cgcggccgat cgctgccatc
     5461 gcgaggtcga cgtcgaagtc gatgtgatca ccggtgacga gcttgatgcc gtggcgcgcg
     5521 atgcccgctt cgacttcacg cgcgagctct ggatcgaagc cgcgcagcac gtgctcgccg
     5581 cgatgcacca gcgtgacgtc gcagccgaag ccgcggaaga tgtgcgcgaa ctcgatgccg
     5641 atgtatccag cgccgaggat gccgatgcgc ttcggcagct ccggcagatg aaagacctcg
     5701 tcgctcgtca catagcgatg cgtgccgccc ggtgggtgat ccgctggaac gcgaggccgt
     5761 ccgccggtcg cgacgagaat gttcgcagcc gtcacgcgct cgccggcgac ttcgatcgtg
     5821 tgcgcatcga cgagccgcgc cctgccgtcg atcacgcgga cgcgcgcttt ctcgaggcgt
     5881 gtgcgatacg ctgccgagag tcgggcgatt tcctggtcct tgagggcgag aaacgtcggc
     5941 cagtcaaacg agacgtccga aatggtccag ccttgtccgc gtgcgtcatc gatcgtgcga
     6001 ctgacctcgc tcgcatagac catcagcttt ttggggacgc acccgcgcac gacgcatgtc
     6061 ccaccccacc gactctcctc ggcaattgcg acgcgagcgc cgtgctcggc cgccacgcgc
     6121 gctgccctca cgccgccgga tccaccaccg atcacgaaga agtcgacgtc gaaccgcgct
     6181 gacatggggt ctgcagtgta cgtgtgctga cgcacccccg catcacgtgc tgtcagtcga
     6241 agccaccgtc aatcagaact cgtgttcaat aaagcagtag acagccccgg cgcggttggt
     6301 acgctcgccc gcgaatgatg agggggatca cgatgcgaag ctcgctgact cttggttctg
     6361 tcgcactgct cgcgctgctc gccgcatgcg gcaccgatcc cgcgatcggt gatgacgatg
     6421 atggcactgg ctccggctcg ggcagcggct cgggttctgg cagcggtatg gatcccgaac
     6481 ctcctgctcg cggctttcag atgaagagcc cggagatcac gatcaaggcg ggacaagaga
     6541 tcacctactg ctggtacttc aagactccga acacggagct cttcacgatc aagcgctggg
     6601 tctcgcacat gaccggcggc tcgcatcaca tgatcgtgtt cttcacgaac tcgctatcgc
     6661 agcccgaagg cacggtctcg tcgagcaact gtggcttcgg cggcggcacg aacgtcggcc
     6721 aatggatcta tgccgcacag aatgaagatg cggacctccc tcttccgtcg gacgacggcg
     6781 ccggcaagcc ggtcggcatg gatgtgaagc ccgcgcagac cggcttcatt cagatgcact
     6841 acttgaacac cgccgatcag gacatcaccg tgcacgtcac gctcaacgga gaagcgtacg
     6901 aagcaggtgt cgatgcgacg aagacgttcg catacgtgac gttcgcgggt gacatcgacg
     6961 tcctgccaat ggcgcagggc tacaagaaca cgcacacgtg tccgattccc gcgacgtcga
     7021 aggtgtggct gatgtcgacg cacgcccatg cgcgcgcggc gcacaccgag gtgcgtgatg
     7081 gcgctgcgac aagcacgaac gtcgtgttcg cgagcgacga ctgggaacat cccggcgcga
     7141 aggcgtggat gacgaacccg ttctacacgt tcgcgagcgg caagctcacc tacgagtgca
     7201 cgtacaacaa cccgtcgtcc aatgagtaca agacgggtga cagcgcgcag accaacgaga
     7261 tgtgtatggc gacgggctat tacttcccgg cgaccaaagc gacgatttgc tacaacagca
     7321 acatcctgcc cttctgagca ggatcgagat cagtcgtcga tctcgatgat gatctcttcg
     7381 ctcatgatca cgtcgtcgtt cgcgggctga gtcggcgccg atgaggccgc cggcttttcc
     7441 tcgtctacct gctcggtgac ggccgccgca ggcgcgtcgt ctttcttcgt ctcggcctca
     7501 tccgacttct cgatcgcagc ggcaacggtg ctgctctcgt gtggcgcgat gctctcgccg
     7561 ccatcgtcct cgggctccgt cttcgccatc tcgggccacg cgggccccgt cgccttggcg
     7621 tgcgagcacg cagacagcgc gaggagaagg agcaagcgac gcatgcgcgt ctagcgtagc
     7681 agctactgga cgcgatagcc caggccgtcg atcgtgggct tgaccaggct ttttgcgtcg
     7741 agcccttggc gcgtcacgat ctcgattttt ttcgtacgaa cgtcggtctt gaccgcgagc
     7801 acgccgtcta cgttggcgag cgccttctcg atgcgcttcg cgcagccgcc gcacttcatg
     7861 ccttcgacgt aaacgggctg ccacgtcgca tccccgccga tcgccgccca ctgcggcatt
     7921 gcgcctgcgc cgccgcacga atcgccgcac gcaccgctgc agcttccgcc gccgccgcac
     7981 gatccgccgc acgacgatgg cgccgcggtg gtcttctccg ctgtcggcgc gggcatcgtc
     8041 cgatcgggat ccggcgacgg cgcgatagca ccgggcgcgg gccgcgcgtg ctgcgtatct
     8101 gccgccgatg ccttcaccga gtccgccttt tggcaggctg cggccatcca cacgatcccc
     8161 acgaacgcga gcaacctcat atcgattcgt gtatcacagc gtcatggtcg aagttggcaa
     8221 gaaagcgcct gcgttcgcgc tcgagtcgtc ggacggcggc accgtgaagc tcaaggagct
     8281 cgagggaaag atcgtcgtcc tctacttcta tccgcgcgac aacacgccgg gctgcacggt
     8341 ccaagccgaa ggcttccgcg atgccatccc cgcgctcaag aagctcggcg cggtcgtgct
     8401 cggcgtcagc aaagacacga tcgcgtcgca ctgcaagttc cgcgacaagt acaagctcaa
     8461 cttcccgctg ctcacggacg ccgacggcgc aacgatggag gcctacggcg cgtggggcga
     8521 caaagtcatg tacggcaaga agatgaaggg catcattcgc tcgaccgtcc tcatcgacaa
     8581 gacgggcaag gtcgcgcagc actggccgaa ggtcagcgtc aaaggccacg tcgacgaagt
     8641 cgtcgatgcg gtcaaggcgt tgaccaagaa gtaacgctca gtggaagtcg cggctgtcga
     8701 cctcgacaac ctcgcgcgag agatcgggat gccagagctg ctcggcgacc agatgcacga
     8761 tgccttcttg cacctgcagt cgcccgctca cgccgagtaa cgacgtcgtg cgaatgatcg
     8821 tcgagtactt gtcgaacacc tgcgcccaga cgacgaggtt cacaaatccg gtctcgtcct
     8881 ccatcgtcat gaacgtcacg cccgacgccg tgcccggttg ctgccggcag atcacgatgc
     8941 cgacgtaatc gatgcgctgg ccatcgcgcc cacgcgacac cgtccgcgca tccggcaacc
     9001 gcttcgctcg cagctcgccg cgcatcggct ccaagggatg tccccgcgtc gaatgatcgc
     9061 tggaccgata gtcccaaaag atctcgtcca tcttggtgag cttgccgaac tgcacgtcgt
     9121 gcacgtcgcc gccgagatcc agcggatcgt cctgccgccg cacccacccg gtcacctgcc
     9181 acagcgcgtc tctccgctcg cgtaccagcg gcccgagcgc gccgcactcc gcgatctgcg
     9241 aatgcgtgcg caccggcaag tgcgtccgcc gcacgaaatc ctcgacgcta tcgaacgccc
     9301 gctcgcgccg cgccgccgtg atcttcgcgc cctccgcgat ctgaatgccc ttgatccaac
     9361 gcagtcccat gcggacggca aactgaaaca cgtcgtcatt cggcagcggc tcgagcgtgc
     9421 aattccactc gctgcacgtg acatcgatcg gcagaatcgt caggccatgc cgctgcgcat
     9481 cgcccacgat cgtcgacggt gaatagaagc ccatcggctg cgcattgagc agcgacgccg
     9541 tgaactccgg caagtagtgc ttgcgcatcc aacacgtcgc atacgcgatg agcgcgaagc
     9601 ttgccgcatg actttccggg aagccgtatt cgccgaagcc gcggatttgc tcgaacacac
     9661 gttcggagaa ttcgcgcgcg atgccttttg cttccatccg tgagatcagg cgctcgcgat
     9721 gtttctcgat gcggcccgag cggcgccacg cggccatgtc tcgacggagt tggtcggcct
     9781 cgcccggcgt gtagtcgcct gccacgaccg cgagccgcat gacttgctct tggaagagtg
     9841 ggacgccgag cgtttttgcg agcaccgact cgaggcacgg gtgcgggtac tcgactttct
     9901 cttcgccttt gcgacgccgc aggtacgggt ggaccatgcc gcccgagatc gggcctggac
     9961 gcacgagcga gacctcgacg acgagatcgt agaacgtgcg cggcttcagg cgcggcagca
    10021 tcgacatctg cgcgcgcgat tcgatctgga atgtgccgac ggtgtctgcc gtgcagatca
    10081 tgtcgtacgt cgccgcgtct tcggcgggga tggtggccat cgagagatcg atgccgcggt
    10141 ggtggcggag catgtcgagg ccgaggtgga gctggtggag cgcgccgagg ccgaggagat
    10201 cgactttgaa gaggccgagg tcttcgagat cgtctttgtc ccattggatg acggtgcggc
    10261 ctggcatcgt cgcgttctcg atcggcacga tgtcgtgaac gggctcgtga ccgaggagga
    10321 agccgcccgg gtggatgccg atgtggcgag ggaactcgag aatttcctcg gagaggcgtg
    10381 cgaggtgatc gagcgcggac gcgtggccgc cttcagcgag gccggagcgc gcgagggctt
    10441 cgtgctcgac ggtgccgtac atcgagagat gtttggcggc gcgatcgagc gcggtttcgg
    10501 gaatgccgag cgctttgccg acgtcgcgga cggccgagcg cggtcggtag cgaatgatgt
    10561 tgcagaccat cgcggcgtga tcgcggccgt agacggagta gacgtgctgg atcacttctt
    10621 cacggcgctc gtgctcgata tcgagatcga tgtcgggcgg ctcggcgcgc tcgcgtgaga
    10681 ggaagcgctc gaacagcagg cccatgcgga ccggatcgac ggcggtgatg ccgaggcaga
    10741 agcacacggc ggagttggcc gcgctgcctc ggccttggca catgatgtct cgacgacggc
    10801 agtacgaaac gatctcgtac atcgtcagga agtagccggg gtagtcgagg tcttcgatga
    10861 ccgagagctc ggcgtcgagc tgcgcgcgaa cgttcggcgg gatgtcgttg tcgtagcgcc
    10921 acgccgcgcc ttcgaacgcg agcttgcgga ggtacgcggc ggatgtgctg ccgtcgggga
    10981 ggcgctccga tggatagcgg tagcggagct cgccgagcga gaacgtgcag cgcgcagcga
    11041 tctcgagcgt gcgtgcaaac gcgccgggct cgtcggcgta gagcttcttg aacgcgtgcg
    11101 gcgggcgaag gtcgtgctcg tcgttgccgc gaatgaggcg gcctgcggtc gcgagcgtga
    11161 cgccgtggcg aatgcacgtg agcacgtctt gcagctggcg gcgcgagcgc gagtgataga
    11221 gcacttcggt cgcagcgacg agcgggatgc gatgtgcagc agcgcgggcg cgcaggcgtg
    11281 cttcacgcgg cacgtcgtcg gcgcggcgat ggcgggacag gatcgcgtag agacgatcgc
    11341 cgaatgcgtg ctgcagatcc gcgacgacgt cgggcggcgg atcgagctca cccgcgagca
    11401 gcgaggtgtc gcctccccac agcgcgatga ggccttgcgc gcgctcgcag act
//
LOCUS       DATLBZ010000117        11213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_114812, whole genome shotgun sequence.
ACCESSION   DATLBZ010000117 DATLBZ010000000
VERSION     DATLBZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11213
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114812"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VMZ53_06225"
     CDS             complement(<1..343)
                     /locus_tag="VMZ53_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28083.1"
                     /translation="MSRWWSVLAVVAFGCGGSDGDDGPNPPPAPSCAPAGGGSTTVAI
                     PTLAYTLADRYQEAWLGSPAVADLDADGVDEIIVPRHELLIVWHPDGTIAWQATLPGR
                     IWASPVVADLVP"
     gene            complement(400..2013)
                     /locus_tag="VMZ53_06230"
     CDS             complement(400..2013)
                     /locus_tag="VMZ53_06230"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28084.1"
                     /translation="MLEGAQIGGYRVVKQIGAGGMGAVWLAEHSALGRRAALKVLHAE
                     YSSRADVVQRFFNEARAATAISDPGIVQIFDFGHHTDGSAYIVMELLDGEPLDRRLAR
                     SGALDVADALRIMRQVASTLGAAHSRGIIHRDLKPENIFMVRDPEVAGGERAKILDFG
                     IAKLIGDPSGSKTQTSAVMGTPTYMSPEQCRGAGHVDQRSDVYALGCVLFMLIVGRPP
                     FDAAGVGEIIAMHLREPAVAPSTMRPGIAAEIDELVLQCLAKDPADRPGSAAELAQAI
                     GALLQSSASPSAARSPLGATMSTGAPIFAKVTTLSAAAGASANIAKPRPNRARAVIAG
                     VASVTVVAVILIVALRRGDETTGAQRLGSAGADEISDATVVVAETVAPVAPVVPVDAS
                     PPSPAALASPSILAALERFAEWSKANAAAPCPDAATLGVVENDAWGHPLQITCTDQPG
                     SQMAGAVSSGPDGVFGTADDIASWELGPEITRVVRGARWPAALATKTATKKKPSSETR
                     VRPTQTQPQTETPKASSLQVDELGLPISR"
     gene            complement(2013..3608)
                     /locus_tag="VMZ53_06235"
     CDS             complement(2013..3608)
                     /locus_tag="VMZ53_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28085.1"
                     /translation="MLKWAFVFVVVGLVGCGFGENDTSGKTASDAISPGCSVDPHGNP
                     LDRNGTVCEGVCLDGACHGTCRPDTVKCNGNQPQKCDANGNFQDEGTMCPFVCAEGAC
                     AGACTPGTLQCNDQTTQSCTAEGTWLDGATCVDQACNGGLCVGTCSPGAFRCVGAEPQ
                     VCNANGEWVSNGATCVAQACDSSTGTCVGACTPDSKRCSGQQPQLCDAQGQWQTNGAT
                     CGNQACVSGVCQGTCSPTTQRCTSSNTHETCSALGSWAADGCTNQACVGSSCTGVCSP
                     GAKRCVGYDQQTCNASGQWAVTQNCAGVCTGSGTCSGVCHPNATRCSGYDSQTCDSAG
                     NWQTSQTCTNVCSGAGSCTGVCHPGTARCNGYNSEVCNSSGAWQLNQTCPNICSGAGS
                     CTGVCHPGATTSCNTQYCTKTRTCQSDGNWGSCNTNSCQTVTQVSNPTAVGWGYCAPQ
                     KILDTEVVTCPGGTVRTRCAAHWDAGFWGTPYDDGDGTDHIYNSKGDIDAQNYGADGC
                     YVHVRAFNCEATTVRAEITCVGI"
     gene            complement(3608..4606)
                     /locus_tag="VMZ53_06240"
     CDS             complement(3608..4606)
                     /locus_tag="VMZ53_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28086.1"
                     /translation="MWCRRIPNTACVLAILATVLAASPAYGQEAGAQAEALFREGREL
                     LAAGRIPQACSAFEQSQKLEPATTTLLNLAGCRELNRQYASAWGYFLQAERETRNSAD
                     PDRIKLHAIAETRAATLEAKVSRLTINVSAESRIEALEVLRGATVVEASAWNRALPVD
                     GGTYTLTARARGATPWTTTVTVGAEADSKTIAIPKLQPAPVEPESDEDGEGEPEQLPP
                     APPRSLVLPIAFGAGAVAMLGGAVAFELSAEATFEQSQAEPNPTTSDILFAKANHRRY
                     AAEALAVTGLAAGGVAVWLYLRTPRDAASSGVASRSVHVVPTVSAAYSGFEMRGSF"
     gene            complement(4720..5487)
                     /locus_tag="VMZ53_06245"
     CDS             complement(4720..5487)
                     /locus_tag="VMZ53_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28087.1"
                     /translation="MTRLAVASDPDLALVSISPQAQTALTYVRGALCNPVSLSVMAFA
                     VCVGVGYAGMLGALIATLAFVTLGTCATRYRFIRHQLDKQFEYRVRQKREADRMKQLK
                     PTGPVRQQQYAELRDLVAEIERQDASEAQRFELQSLLDHFIQLAVSHQKCLDSLRLAG
                     SSDLPRSLPLPDPNRSHRRREIMQRRLRHRDECVRRIERLADELEAVDELVRLVAQRV
                     ACPALEGELDREIERRLWELDEVDAALHSLSATTTAA"
     gene            5734..6288
                     /locus_tag="VMZ53_06250"
     CDS             5734..6288
                     /locus_tag="VMZ53_06250"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HUS28088.1"
                     /translation="MALSATMRRFTIALSDVDRGVYEELEWRVAQHPSESERYLVARI
                     LARALEHGEGLEFSKGGVSDDEEPALVQRDLRGDLLAWIEIGSPTPDRLHKAAKVTKR
                     VVVYTWKSPEQLAEAIAERKVHRAEHIELRGLDPDWLDRVASTLDRNNTWDLSVTGGA
                     IYLTIGSELFETTVSVSGSASRSD"
     gene            complement(6260..7588)
                     /locus_tag="VMZ53_06255"
     CDS             complement(6260..7588)
                     /locus_tag="VMZ53_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28089.1"
                     /translation="MRHVGKIASALSLACIAAGCASEGGGSDENPFLQDLSDSGKEDS
                     AYMNPDGIEVEVDLEGDVEGPDYRLKDAPAEIGQFALTFFRKQEKMFIESLAEDASTT
                     NRAEWLVNGTWYTADRVPAGSTLKHWRLKGLNTVLLFTASSGVQVGTTFTAKVPLKPF
                     SVFTDAQKTCADSDDHIGLDQSVYWYRWEPEQTSCKIPLQDLKVTVSKKFSSTTATVY
                     PEYDQLMADKKVTMVVLFGEIDDGPITENETGMRNFKRFGSWLTGAGFTKAATAPLGQ
                     RFSKKLGNGTTVEIDLYSPREFSGLGDFSHFNNFQKALSEHEVIAYDGHSMLGASDFW
                     ARPTYPSFYQIFLYGGCLGYEYYVKPILHGKGDSWAKLDIVSSVVEVTASANEFAGPA
                     IAKIIYGIEHNGRSSWRNIMLNVRNSVGDSTFGVSGVRENCFSPSGTRCH"
     gene            complement(7714..8883)
                     /locus_tag="VMZ53_06260"
     CDS             complement(7714..8883)
                     /locus_tag="VMZ53_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28090.1"
                     /translation="MVARGAGDVGCAHCTQFGARKTCRVCKLQVCDVCAADWTTCSEP
                     AGRELRLGMGWRVRDVDPKGRFAFVTHILKAPRIVELRQLRWFGPAPIGLNLYNSLRS
                     APPRLTSDGLFVHADVSLVNDSTYIDGVRWHTLGATRVAVHVKDVEGPHSSTCVSAVN
                     DTFAYVTDSQRVVVMRHGPMTMDALPPAAAVVTHPGVVRHDPSLLFRFVSETYEPLPR
                     KVISAIDLDGERDLLASVTWRELAIHRTVGGKLERVAHLDTGIHANLTFVALTGRWLA
                     YGTHSCIELRPMKHDGSIGEVVQRHSGRIGAAALSRDGAYLAVAIDSKLVVEDLERFS
                     TAEFTEHSDGINYVRFASDDHMLISGDQDNRIVVRPRTAAGYAVPVIDVELAGEP"
     gene            complement(8886..>11213)
                     /locus_tag="VMZ53_06265"
     CDS             complement(8886..>11213)
                     /locus_tag="VMZ53_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS28091.1"
                     /translation="RLTSGTTWHAAGLIVTFGSTSETSTEMRKYTRDLYARLEAETGQ
                     STGFKPVGFIEVAGDKDRLEEYRRVSAFNRVCGVDVHEISPGQIKDLFPIAKTDDLLA
                     GFYVKEDGRVNPVDATMALAKGARMQGAKLVENCPVTGFRTKRGNAGAEVTGVVTPYG
                     EIECEYVVLCGGMWSRQLAATVGVNIPLQAAEHYYLITERMSQISPDWPVLEDPGSYG
                     YFREEVGGLMIGLFEPKCAPWNVGGIPNDFSFGEIQPDWERMGPYVEKAMARVPISMD
                     TGVKKFFCGPESFTPDMAPIVGDAPELKNFFVCAGLNSIGIITGGGLGRVMAHWLMNG
                     RPDVDVSYMNIDRLHTYQANPEYRRLRTVESLGMVYQCHYPTRSMMTARGAKKSPFYD
                     RLAARGAYFRDVSGWEGADWYGNPDNTLTFGKPSWFPQWAAEHKAAREGVILMDMSFM
                     SKFLVEGRDAGRLLNWISTNNVDGDAGQITYTQWLDETGKLQADLTVAKLDEQKYWVV
                     ASDTAHRHVQTWMKRHFGDAHAFVADQTSGYAQINIQGPRSRELMQLATSVDMGNEAF
                     PFRAAREIDIGLARVLCIRITYLGELGYELYIPTEQALHVYDRLVELGERVGLVHAGL
                     KTLASCRMEKGYRDYGHDIDNTDTILEAGLGFAADLKKPGGFLGKDAVVAQKAQGTLK
                     KRLLQILVKDPEPMLFHAEPVKRDGKVVGYIRAASYGHTIGGAVGLAMIQADEPIDPK
                     WIAAGTWEVEIAGTTYPALASLKPLYDAENKQVKL"
BASE COUNT         1847 a         3771 c         3601 g         1994 t
ORIGIN      
        1 tcggcacgag atccgcgacc acaggcgacg cccaaatgcg ccccggcaac gtcgcctgcc
       61 acgcgatcgt tccatcggga tgccacacga tcagcagctc gtggcgcggc acgatgatct
      121 cgtcgacgcc atctgcatcg agatccgcga cagccggcga gccgagccac gcttcttgat
      181 agcgatcggc aagcgtatac gcgagcgttg ggatcgcgac ggtcgtcgag ccaccaccgg
      241 cgggcgcgca cgatggcgca ggcggcgggt tcggcccatc gtcgccatcg ctgccgccac
      301 atccgaatgc gacaacagcg agaacgctcc accagcggct catgctccga cgataacgcg
      361 gtctccgggc cgctcgcgac gtagttgaag gtgctaccgc tagcgcgaga ttggcagccc
      421 gagctcgtcg acttggagcg aagaggcttt cggagtctcg gtctgcggct gcgtctgcgt
      481 cggacgcacg cgcgtctcac tgctcggctt cttcttcgtc gcggtcttcg ttgcgagcgc
      541 cgcaggccac cgcgcgccgc gcacgacgcg cgtgatctcc ggccccagct cccacgacgc
      601 aatgtcgtcg gccgtgccga acacaccgtc gggcccgctc gaaaccgcgc ccgccatctg
      661 agatccaggt tggtcggtgc acgtgatctg caacggatgt ccccatgcgt cgttctcgac
      721 cacgccgagc gtcgcggcat cggggcacgg tgcagcggcg tttgctttcg accactctgc
      781 aaaccgctcg agcgcggcga ggatcgaggg cgaggccagt gcagctggac tcggaggcga
      841 cgcgtcgacg ggcacgacgg gcgcgacggg cgcaaccgtc tcggcgacga cgaccgtggc
      901 atccgagatt tcatcggcac cggcggagcc gaggcgttgc gcaccggtgg tttcgtcgcc
      961 gcgtcgaagc gcgacgatca ggatgactgc gacgacggtg acgcttgcga cgcccgcgat
     1021 cacagctcgc gcgcggttcg ggcgcggctt cgcgatgttc gcgctcgcgc cagccgccgc
     1081 cgacagggtc gtcacctttg cgaagatcgg tgcgccggtc gacatcgtcg cgccaagcgg
     1141 cgagcgcgct gcgctcggtg atgcactgct ctgcagcagt gcgccgatcg cctgcgcgag
     1201 ctcagctgcg ctgccgggcc ggtccgccgg atccttcgcg agacattgca agacgagctc
     1261 gtcgatctcc gctgcgatcc caggccgcat cgtcgacggt gcaacggcgg gctctcgcag
     1321 atgcatcgcg atgatctcgc cgacgccggc ggcatcgaac ggcggacgcc cgacgatgag
     1381 catgaacagc acgcaaccga gcgcgtacac atcgctgcgc tgatcgacgt gccctgcacc
     1441 gcgacattgc tccggcgaca tatacgtcgg cgtgcccatc accgccgagg tctgcgtctt
     1501 cgagcccgat ggatcgccga tgagcttcgc gatcccgaag tcgaggatct tcgcgcgctc
     1561 tccaccggca acttccggat cgcgcaccat gaagatgttc tccggcttca agtcgcgatg
     1621 gatgatcccg cgtgagtgtg cggcgccgag cgtgctcgcg acttgccgca tgatgcgaag
     1681 cgcgtccgcg acatcgagtg caccggaccg cgcgagcctg cgatcgagtg gctctccgtc
     1741 gagcagctcc atcacgatgt atgcactgcc gtccgtgtgg tgcccgaagt cgaagatctg
     1801 aacgatgcct ggatccgaga tcgccgtcgc ggcgcgcgcc tcgttgaaga accgctgcac
     1861 gacatcggcg cgactcgaat actcggcgtg caacaccttg agcgctgcac gccggccgag
     1921 cgcgctgtgc tcggcgagcc agactgcgcc catcccaccg gcgccgatct gcttgacgac
     1981 tcgatagccg ccgatctgcg cgccttccag cattagatac ccacgcaggt gatttctgcg
     2041 cgcaccgtcg ttgcttcgca gttgaacgcg cggacgtgga cgtagcagcc atcggcgccg
     2101 tagttctgcg cgtcgatgtc gcccttgctg ttgtagatgt ggtcggtgcc gtcaccgtcg
     2161 tcgtagggcg tgccccagaa gccagcgtcc cagtgagcgg cgcaacgggt gcggacggta
     2221 ccgcccgggc aagtcacgac ctcggtgtcg agaatcttct gcggcgcgca gtagccccag
     2281 ccgaccgccg tcggattgct gacctgcgta accgtctggc agctgttcgt gttgcacgag
     2341 ccccagttgc catcgctctg gcacgttcgc gtcttcgtgc agtactgcgt gttgcacgac
     2401 gtggtggcgc cgggatgaca cacgccggtg cacgaacccg caccgctgca gatgttcggg
     2461 cacgtttggt tcagctgcca tgcgcccgac gagttgcaga cttccgagtt gtagccgttg
     2521 caccgcgcgg tgcccggatg acacacgccc gtgcacgaac ccgctccgct gcacacgttc
     2581 gtgcacgttt ggctcgtctg ccagttaccc gccgagtcgc acgtctgcga gtcgtagccc
     2641 gagcagcgcg tggcgttggg gtgacagacg ccggagcacg ttcccgagcc agtgcacacg
     2701 cccgcgcaat tttgcgtgac cgcccactga ccactcgcat tgcacgtctg ctggtcgtag
     2761 cccacgcagc gtttcgcgcc tggcgagcag acacccgtgc agctcgagcc gacgcacgct
     2821 tggttggtgc agccatccgc cgcccagctt ccgagcgcgc tacacgtttc gtgcgtgttg
     2881 ctgctcgtgc accgctgcgt cgtcggtgag cacgtgcctt ggcacacacc actcacgcac
     2941 gcttggttgc cgcacgtcgc gccgttggtc tgccactggc cttgcgcgtc gcacagctga
     3001 ggctgctgac cgctgcaacg cttcgagtcc ggcgtgcacg cgccgacgca ggtgccggtg
     3061 ctggaatcac acgcttgagc gacgcacgtt gcgccattgc tcacccactc gccattcgcg
     3121 ttgcacacct gcggttcggc gccgacacag cggaacgcac cgggcgaaca cgtgccgacg
     3181 cacagccctc cgttacatgc ttggtcgacg cacgtcgcgc cgtcgagcca tgtgccctcc
     3241 gccgtacacg actgcgtcgt ttgatcgttg cactgcagcg ttcccggtgt acacgcgccc
     3301 gcgcatgcgc cttccgcgca gacgaacgga cacatcgttc cttcgtcttg gaagttgccg
     3361 ttcgcgtcgc atttctgcgg ctggtttcca ttgcacttca cggtgtccgg acgacacgtg
     3421 ccgtgacacg cgccgtcgag gcatacgcct tcgcagacgg tgccgttgcg atcgagcgga
     3481 ttgccgtgcg gatcgacgga gcagccggga ctgatcgcgt cgctcgcggt cttgccactc
     3541 gtatcgttct cgccgaagcc gcagccgacg agaccgacca ccacaaatac aaaagcccat
     3601 ttcaacatca gaagctcccc ctcatttcga agccggaata agcagccgac accgtcggca
     3661 cgacgtgaac gcttcgcgat gcaacgccac tcgacgccgc atcacgtgga gtgcgcaggt
     3721 acagccacac cgcgacgcca cccgccgcga gcccggtcac cgcgagcgcc tcggcggcgt
     3781 agcggcgatg gttcgctttc gcgaacagaa tgtcgctcgt cgtcggattg ggctcagcct
     3841 gtgattgttc gaacgtcgct tctgccgaca gctcgaacgc gacggctccg ccgagcatcg
     3901 cgacggctcc ggcgccaaac gcgatcggca gcaccaagct gcgcggcggt gcaggaggca
     3961 gctgctcggg ttcgccttcg ccgtcttcgt cactctccgg ctcgaccggt gcgggctgga
     4021 gctttgggat cgcgatcgtc ttcgagtcgg cttccgcgcc gacggtcacc gtcgtcgtcc
     4081 acggcgtcgc gccccgcgcc cgcgcggtca gcgtataggt tccgccatcg acgggcagcg
     4141 cgcggttcca tgcgcttgcc tcgacgaccg ttgcaccgcg cagcacttcg agcgcttcga
     4201 tgcggctctc cgcgctcacg ttgatcgtca gccgcgagac cttcgcttcg agagtcgccg
     4261 cgcgcgtctc ggcgatcgcg tgcagcttga tgcggtccgg atccgcggag ttgcgcgtct
     4321 cgcgttccgc ctgaaggaag tagccccacg cgctcgcgta ctggcggttc agctcgcggc
     4381 agccagcgag gttcaagagc gtcgtggtcg cgggctcgag cttctggctc tgctcgaacg
     4441 cgctgcacgc ctgcgggatc cggccggccg ccaagagctc gcggccctct cggaacagcg
     4501 cctcggcctg ggccccagct tcctggccat aggcaggcga ggcggccagt acggtcgcga
     4561 ggatcgcgag gacgcacgcg gtgttaggga tacgtcggca ccacatctcc ccgggataga
     4621 gacggctcgc cggcgatgtg aaaaagaaat ttgaaggggc gccgaaaaaa ccgaacggga
     4681 gctcgtgaaa gctcccgttg gggacacgtt ggggtggcgt tacgcggccg tcgtcgtcgc
     4741 cgagagcgag tgcagggcgg cgtcgacttc gtcgagctcc cagaggcggc gttcgatctc
     4801 gcgatcgagc tcgccttcga gtgccgggca cgcgacgcgc tgcgcgacca ggcggaccag
     4861 ctcgtccaca gcttccagct catcggcgag acgctcgatg cggcggacgc attcgtcgcg
     4921 atggcggagg cggcgctgca tgatctcgcg gcggcggtgc gagcggttcg ggtcgggcag
     4981 cggcagcgag cggggcaggt cgctcgagcc cgcgaggcgg agcgagtcga ggcacttctg
     5041 gtggctgacg gcgagctgga tgaagtgatc gagcagcgac tgcagctcga agcgctgcgc
     5101 ctccgatgca tcctggcgct cgatctcggc gacgaggtcg cggagctcgg cgtactgctg
     5161 ctggcgaacc gggccagtcg gcttgagctg cttcatgcga tcggcttcgc gcttctggcg
     5221 gacgcggtac tcgaactgtt tgtcgagctg gtggcggatg aaccgatagc gcgtcgcgca
     5281 cgtgccgagc gtgacgaacg cgagtgtcgc gatcagcgcg ccgagcatcc ccgcgtaccc
     5341 gactccgacg cagacggcaa atgccatcac ggagaggcta acggggttgc acagagcgcc
     5401 acgtacgtac gtgagcgccg tctgtgcctg cggtgagatc gacacgaggg cgaggtcggg
     5461 gtcggaggcc actgcaagcc gcgtcatggg acaagccttc gtacaatccg cgtgccgccc
     5521 gtcgagccga gaggcctcgt ggcgttttga cccaagagct ggtcgcccac gacacaccgt
     5581 ggcgattgcg actggtcctg atcggaacat cgcgatcccc ggtcgtgatc catcccgttc
     5641 ttcgtcggtg agaccgcctc tcgaccgtgc gctagtgcga cgccggccgc cgttcacacc
     5701 tacatcggtt cgagctgggt gatactcgcg cggatggcac tttccgccac catgcgacgc
     5761 tttacgatcg cgctctccga tgtcgaccgc ggcgtctacg aagagctcga gtggcgcgtc
     5821 gcgcaacacc cttcggagag cgagcggtat ctcgtcgccc gcatcctcgc gcgtgcgctc
     5881 gagcatggcg aaggcctcga gttcagcaag ggcggcgtga gcgacgacga agagcctgcg
     5941 ctcgtgcagc gcgatctgcg cggcgatctc ctcgcctgga tcgagattgg atctccgacg
     6001 cccgatcggc tgcacaaagc ggcgaaggtg acgaagcgcg tcgtggtcta tacgtggaag
     6061 agcccagagc aactcgccga ggcgatcgcc gagcgcaagg tccatcgcgc cgagcacatc
     6121 gagctacgcg gtctcgatcc agactggctc gatcgcgtgg cgtctacgct cgatcgcaac
     6181 aacacctggg acctttcggt cacaggtgga gccatctatc tgacgatcgg tagcgagctg
     6241 ttcgaaacca ctgtcagcgt tagtggcagc gcgtcccgct cggactgaag cagttctcgc
     6301 gcacacccga gacaccgaac gtcgagtcgc cgacgctatt gcgaacgttc agcatgatgt
     6361 tgcgccacga gctgcggccg ttgtgctcga tgccgtagat gatcttcgca atcgcggggc
     6421 ctgcgaactc gttcgcgctc gcagtcactt cgacgaccga ggacacgatg tcgagcttcg
     6481 cccacgagtc gcccttaccg tggaggatgg gcttcacgta gtactcgtag ccgaggcagc
     6541 cgccgtacag gaagatttga tagaagctcg ggtacgtcgg gcgagcccag aagtcagacg
     6601 cgccgagcat cgagtgaccg tcgtatgcga tgacctcgtg ctccgagagc gccttctgga
     6661 agttgttgaa gtgcgagaag tcgcccaagc ccgagaactc gcgcggcgag tagaggtcga
     6721 tctcgaccgt cgtgccgttg ccgagcttct tcgagaagcg ctgaccgagg ggcgccgtcg
     6781 cggccttcgt gaagcctgca ccggtgagcc agctgccgaa gcgcttgaag ttgcgcatgc
     6841 cggtctcgtt ctcggtgatc gggccgtcgt cgatctcgcc gaacaggacg accatcgtga
     6901 ccttcttgtc ggccatcagc tggtcgtatt ccggatagac cgtcgcggtc gtcgacgaga
     6961 acttcttcga gacggtgacc ttgagatcct gcagcgggat cttgcagctc gtctgctccg
     7021 gctcccagcg gtaccagtag acggattgat cgaggccaat gtggtcatca gaatccgcgc
     7081 aggtcttctg tgcatcggtg aagacgctga acggcttcag cggaaccttc gcggtgaacg
     7141 tcgtgccgac ttgcacgccg ctcgacgcgg tgaacaggag cacggtgttg aggcccttga
     7201 ggcgccagtg cttgagcgtc gagcccgcgg ggacgcgatc cgccgtgtac cacgtgccgt
     7261 tgacgagcca ctccgcgcga ttcgtggtcg acgcgtcttc cgcgagcgac tcgatgaaca
     7321 tcttctcttg cttgcggaag aacgtgagcg cgaactggcc gatctcggcg ggcgcatcct
     7381 tgaggcgata gtccggaccc tcgacgtcac cttcgagatc gacttcgacc tcgatgccgt
     7441 ccgggttcat gtacgcggag tcttccttgc cgctgtccga gaggtcctgg aggaacgggt
     7501 tctcgtcgct cccgccgcct tcgctggcgc aaccggccgc gatgcaggca agggacaggg
     7561 cagaggcgat ctttccgacg tgacgcatgg tgcccgggca aggagcaagg gcgaggccac
     7621 gagacgcgcc agttctctcg cgcacttggc gcattttgat ccggctagcc gaggagcctg
     7681 ttggcggctg tagccgcctg aaaaggcctc ggtttagggc tcgcccgcga gctcgacgtc
     7741 gatgaccggc accgcgtagc cggcggctgt ccgcggccga acgacgatgc ggttgtcctg
     7801 gtcgccactg atcagcatgt ggtcgtcgct cgcgaaccgg acgtagttga tgccgtcgga
     7861 gtgctccgtg aattccgctg tcgagaagcg ctcgagatct tcgacgacga gcttgctgtc
     7921 gatcgcgacc gcgagatacg cgccatcgcg gctcagcgcg gccgcgccga tgcgacccga
     7981 gtggcgttgc acgacttcgc caatcgagcc gtcgtgcttc atcggccgca gctcgataca
     8041 agaatgcgtg ccgtacgcga gccagcgacc ggtgagcgcg acgaatgtca ggtttgcatg
     8101 aatgccggta tcgagatgtg cgacgcgctc gagctttccg ccgacggtcc gatggatcgc
     8161 cagctctcgc cacgtcacgc ttgcgagcaa gtcgcgctcg ccgtccagat cgatcgccga
     8221 gattactttt cgcggcagcg gctcgtacgt ttcggagacg aagcgaaaca ggagcgacgg
     8281 atcgtggcgc acgacacctg gatgcgtgac gaccgctgca gctggcggca acgcgtccat
     8341 cgtcatcggg ccgtgccgca tcacgacgac acgctgcgag tcggtgacgt atgcgaacgt
     8401 gtcgttgacg gccgatacgc acgtgctgct gtgcgggcct tcgacatcct tgacgtgaac
     8461 tgcaacgcgc gttgcgccga gcgtgtgcca gcggacgccg tcgatgtacg tgctgtcgtt
     8521 gacgagcgag acgtccgcat gaacgaacaa gccgtcgctc gtgagccgcg gcggtgcact
     8581 cctgagcgag ttgtacaagt tgagcccgat cggcgcggga ccgaaccatc gaagctgacg
     8641 cagctcgacg atgcgcggcg ccttgaggat atgcgtgacg aacgcgaacc ggcccttcgg
     8701 atccacatcg cgcacgcgcc accccatgcc gagccgaagc tcgcgacccg caggctcgct
     8761 gcatgtcgtc cagtccgcgg cgcagacgtc acagacctgg agcttgcaga cgcggcacgt
     8821 cttgcgtgca ccgaactgcg tgcagtgggc gcacccaaca tcgcccgcgc cacgcgcgac
     8881 catggctaca gcttaacttg cttgttctcg gcgtcgtaca gcggtttgag cgacgcgagc
     8941 gcgggatacg tcgtgcctgc aatctcgact tcccacgtgc ccgccgcgat ccatttcgga
     9001 tcgatcggct cgtccgcctg gatcatcgcg agtccgaccg cgccgccgat cgtgtggcca
     9061 tacgacgccg cacggatgta accaacgacc ttgccatcgc gcttcacggg ctcggcatgg
     9121 aacagcatcg gctctggatc cttcacgagg atctggagca gccgcttctt cagcgtgccc
     9181 tgcgccttct gcgcgacgac cgcatccttg ccgaggaagc cgcccggctt cttcagatcc
     9241 gcggcgaagc caaggcccgc ttcgaggatc gtgtcggtgt tgtcgatatc gtgaccgtag
     9301 tcgcgatagc ccttctccat tcggcagctc gcgagcgtct tgagacccgc atgcacgagc
     9361 ccgacgcgct caccgagctc gacgagtcga tcgtagacat gcagggcctg ctccgtcggg
     9421 atgtacagct cgtagccgag ctcgccgaga tacgtgatgc gaatgcagag cacccgcgcg
     9481 aggccaatat cgatctcgcg cgccgcgcga aacgggaacg cttcgttgcc catgtcgacg
     9541 ctcgtcgcca gctgcatcag ctcgcgcgac cgcggcccct ggatattgat ctgcgcgtag
     9601 ccgctcgtct gatcggcgac gaacgcatgc gcatcaccga agtgccgctt catccacgtc
     9661 tgcacgtgac gatgtgcggt gtcgctcgcc acgacccaat acttctgctc gtcgagcttc
     9721 gcgaccgtga ggtccgcttg cagcttgccc gtctcgtcga gccactgcgt gtacgtgatc
     9781 tgcccggcgt cgccgtcgac gttgttcgtg ctgatccagt tgaggagccg ccccgcatcg
     9841 cgtccctcga cgaggaactt cgacatgaac gacatgtcca tcaggatcac gccctcgcgc
     9901 gcagccttgt gctccgccgc ccactgcgga aaccagctcg gcttgccgaa cgtcagcgtg
     9961 ttgtccgggt tgccgtacca atccgcgcct tcccatccgc tcacgtcgcg gaaatacgcg
    10021 ccgcgtgcgg cgagtcgatc atagaacggc gacttcttcg cgccgcgcgc cgtcatcatc
    10081 gagcgcgtcg ggtaatggca ctggtacacc atgccgagcg actccacggt gcgcagccgc
    10141 cggtactcgg gattcgcctg atacgtgtgc agtcgatcga tgttcatgta gctgacatcg
    10201 acgtcgggcc ggccattcat cagccagtgc gccatcacgc ggccaagccc gccgcccgtg
    10261 atgatgccga tcgagttgag ccccgcgcag acgaagaaat tcttcagctc gggcgcatcg
    10321 ccgacgatcg gcgccatgtc gggcgtgaag ctctcgggcc cgcagaagaa tttcttcacg
    10381 ccggtgtcca tcgagatcgg cacgcgcgcc atcgccttct cgacgtaggg ccccatgcgc
    10441 tcccaatccg gctgaatctc gccgaacgag aagtcgttcg ggatgccgcc cacattccac
    10501 ggcgcgcact tcggctcgaa gaggccgatc atcaagccgc cgacctcctc gcggaagtag
    10561 ccgtacgagc ccggatcctc gagcaccggc cagtccggcg aaatctgcga catgcgctcg
    10621 gtgatcaagt agtagtgctc cgccgcctgc agcgggatgt tcacgcccac cgtcgccgcg
    10681 agctgccgcg accacatgcc gccgcacagc acgacgtact cgcactcgat ctcgccatac
    10741 ggcgtgacga cgcccgtcac ctccgcgccc gcattgccgc gctttgtccg gaacccggtc
    10801 accggacaat tctcgacgag cttcgcgccc tgcatgcgcg cgcccttcgc gagcgccatt
    10861 gtcgcatcga cgggattcac gcgcccgtcc tctttcacgt agaagcccgc gagcaagtcg
    10921 tccgtcttcg cgatcgggaa gaggtccttg atctgccctg gactgatctc gtggacgtcg
    10981 acgccgcaca cgcggttgaa cgccgacaca cgccgatatt cctcgagccg atccttgtcg
    11041 ccggcgactt cgatgaagcc aacgggcttg aagcccgtcg actgcccggt ctccgcttcg
    11101 agtcgtgcgt agaggtcgcg cgtgtacttg cgcatctccg tcgaggtctc gctcgtcgag
    11161 ccgaacgtga cgatgaggcc ggccgcgtgc cacgtcgtgc cgctcgtcag gcg
//
LOCUS       DATLBZ010000118        19059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8255270, whole genome shotgun sequence.
ACCESSION   DATLBZ010000118 DATLBZ010000000
VERSION     DATLBZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19059
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8255270"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VMZ53_06270"
     CDS             complement(<1..361)
                     /locus_tag="VMZ53_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUS28092.1"
                     /translation="MRAALLVAVAACTPAASKVAAPPAQPAPAAAPAPDDGQPKWIGV
                     VFDAGTARVGQVIPGSPAYDAGIRRDDVVITFDGHPITIGKEVPPLVQQHAAGSTATI
                     VIKRGTAEQTLQLRIQPR"
     gene            complement(358..1347)
                     /locus_tag="VMZ53_06275"
     CDS             complement(358..1347)
                     /locus_tag="VMZ53_06275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUS28093.1"
                     /translation="MQTRKLGRNGPSVGAIGLGCMGMSAFYGPLDDAESLATIDRAIE
                     LGVTLLDTSDMYGPHTNEQLLGKALKGSKRDKVVLATKFGIVGSVDPTKRRVDGSPAY
                     VKSACDASLSRLGMDHIDLYYQHRVDASTPIEDTIGAMADLVRAGKIRYIGMSEAGPE
                     TLRRAAKVHPIAALQTEYSLWSREPEDGLLALCRELGIGFVAYSPLGRGFLTGQIKKP
                     EDLAADDWRRGMPRFQGENFAKNIELVDHITRLAQQKGVTPGQLALAWVLSRGDDIVP
                     IPGTKRRAYLEENVAADAIRLTADELAEIDRIAPKDVASGMRYPEAAMRSVNL"
     gene            1375..1932
                     /locus_tag="VMZ53_06280"
     CDS             1375..1932
                     /locus_tag="VMZ53_06280"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HUS28094.1"
                     /translation="MWRVPTALETEPFRHLIIDDACERPDDLLAIVDDEGVEPYEGDI
                     FRFEATTPESRGLHSIREQFAAELAPALSRLTGRTVRRVDMRAYAYRPGHYLLPHTDH
                     REGLERVLAYAYYLPTPEPPKGGELELFACTLADDELTSTISAKVIEPRANRLVIFEV
                     GDTSLHQVREVLAGMRLSLAGWFYA"
     gene            1925..2434
                     /locus_tag="VMZ53_06285"
     CDS             1925..2434
                     /locus_tag="VMZ53_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28095.1"
                     /translation="MPELPRLEDLFGTALVIPDAIPRGQLAMARERLTGWQRYALLDR
                     GSYEWIDDVDVLRREIVELAERVTSRTGLAIRECRALRLVAGDYLLAHHDRLHDDNPV
                     EVMVDLSPAPASAEVHYRRRGQTFLRVPSVPGTAAVVERGPSITCNHTYVSKRDPATC
                     AIRLVALLK"
     gene            2444..2821
                     /locus_tag="VMZ53_06290"
     CDS             2444..2821
                     /locus_tag="VMZ53_06290"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HUS28096.1"
                     /translation="MATTKRADFGKPVDGYIKKRSPDQQKILTELKKLITAAAPECEA
                     QIKWGMPHYTIGGKYYAATPAHKAHVNLIMFGPAKSFDDPDGLLEGEGKVGRHLKIAT
                     AKELPKAKIKKWLAIAAKASRSA"
     gene            complement(2844..3737)
                     /locus_tag="VMZ53_06295"
     CDS             complement(2844..3737)
                     /locus_tag="VMZ53_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28097.1"
                     /translation="MRRFAACVLVVLGCGHESTSTETPDASSSSESDAAVVEPDAPAA
                     DDPTACVQWTTWPEQQPSLGQPSWGTQLTDIARHLPAQYGDQYWSDDAITAGHETSHG
                     IHAHLRNYVAPQPIGYNAFYVLNGKVAIVAEPDMRKSDIKPRVAMVLRGPRFHLYLEQ
                     QTEWDDTPLYVFDEWNAYVNGAEVGVGQVQAGLYDGQWTDAVMGPLEFTVYALATAAT
                     AKDKDPAYYTTNTQFRCFTAYNIKRAMKLYETGHLMNEFSWQTQEQYAVTLRSSPAAE
                     PLRVVARELWGADWTQSVLGF"
     gene            complement(3785..4465)
                     /locus_tag="VMZ53_06300"
     CDS             complement(3785..4465)
                     /locus_tag="VMZ53_06300"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HUS28098.1"
                     /translation="MKIALLVVAGAAIVAAVLWLPAGEWIRAAVTWVRGTGVLGIVVF
                     SLVFIALAVTILPTMEMYLAAGMLYGAIWGALLMNSLGLVVELCTLLIVKTSLRKRIE
                     PMIERHPKLQALDTAIRKNSLSILFLLRLSPLMPFGVLNYALAMTKVPLWKRLVTNFV
                     GMLPCTLMLTYVGSYLSSVTQLSNAQPPSVWKHVALWGGLATTIAATWLAARATRHAL
                     HEQHPEAC"
     gene            4558..7359
                     /locus_tag="VMZ53_06305"
     CDS             4558..7359
                     /locus_tag="VMZ53_06305"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS28099.1"
                     /translation="MNRRRYIVGGAALIAAIAVVAAIAQRQRTVTSATHDNTAPVRSL
                     KSVVLAPTSSVAGVIRDTAGASLAGATVCAQTRDRSISCATSNADGAYAIDHLFAATY
                     EVSAAAPKFRPTSYHPGGDPLRTRFALGEGERRTGIDIALDGTAVELRGTVLDVSGGP
                     IAHARVRVAHENVNINEWRHWYPATETADDGTFTLWVAAGERRLEASADGYASVEQDA
                     QAPGRVELLLIPGGSIAGTVVDHEGKPVADALVVADWIDEHKRARSDDTGHFEITGLV
                     PARYDVSAHTANAYGTAVGSVLVGLAAHVDGIIVRVDPAAHIVGRVVTKPPSTLCPAA
                     DLDVTLHDPFGDNIEMTVAADGTVHADGVMASTYYANVRCRGYIAADRYPPVVIAAAD
                     QELTWNVAPGITLHGHVRTPDGAGIEDAIVEVRNGFHSYDATSSRDGSYEIGGIRPGV
                     YRVWASSEYADVTNTEMTLDSNVEKDFVLGVPATIRGIVVDADNHPVSSVEIRAEGKG
                     LAGRLDASRPDGTFELRLAPGTYDVYAAVDWRHPLGKKERVTVSVDKPASVRLVVPAQ
                     TGTIKGDVSDAFGRPVSDAFVYAYTPSMFGNDELQIGWNEHPTLTAPDGTFTITGLGP
                     GTYTVIAQRRGGGSTRVTDIERGSKVSLVLAKTASLSGRISYTDHTHPSQMKLTVRAD
                     TAADRFFREQRFYHTDGVFTLDELPAGTVTIAVETPDGLGVASVLLTAGEQRRDVALT
                     VDRSVTITGRVVEQLTHKGMASCFVIPRSTRALLGAVADYMDVTHQTDADGTFKMLAP
                     PGEVALEISSTDETDRELCAPPVRLDLRSSLNVGDIALVRPRTKIPSNVGFTLSGGTV
                     DSVAPKGPAAAAGLRVGDVVTSIDGVGPASFVAECGFALIAVPPRTTLTLTLARGDTI
                     KITSGDQPAGDEIEIDEQP"
     gene            7391..7645
                     /locus_tag="VMZ53_06310"
     CDS             7391..7645
                     /locus_tag="VMZ53_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28100.1"
                     /translation="MRLRTISALLVGATVAAVLISSHTEHGQLANQARINRNASSEVL
                     KFHAVLQARIDIQLSQPDLGTPTQAPDIWRLPPIQRQRVG"
     gene            complement(7665..8579)
                     /locus_tag="VMZ53_06315"
     CDS             complement(7665..8579)
                     /locus_tag="VMZ53_06315"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HUS28101.1"
                     /translation="MDIFDEAAKPDLQPLSAFLPGSTEFPAPLADKLRASLARDIDTT
                     PQTIEAADRAQTQLTDLLLEHGHRAAAFLDVYHGITVAVIDALGRGVLTPKPFFVRLA
                     GRFAERHFDGLKADLGLDTTSDAARYRLWRPSFAFDNLSPDAATPLATKPAMAHFTVG
                     MCQHINFDLACALDETIRELRFAHDKAALEQIENGHNFVDTILAEKVERSTELLAAKF
                     DCPMSKKIIESGGVKSVGEISMTMIRRWRAKTFVHALQLSSAPDEAARATIREQIYRT
                     NARKTVRLFNTLPNLIEGTLRGTWFATS"
     gene            complement(8590..9165)
                     /locus_tag="VMZ53_06320"
     CDS             complement(8590..9165)
                     /locus_tag="VMZ53_06320"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUS28102.1"
                     /translation="MWPRLGSSSFTTQWIVATLTCSLVAVFDGGWLARWFALAPHRIW
                     HGEVWRLVTWPLVEPGPLGLVITCLAIYKFGGELAVRWGDRRLRRFIIEVVVAASVVT
                     CLVAAVSGRFVFRLGGWAMVDTLVIAWARQFPQRALVVYGMVTLRGRDLIRLTIAVAI
                     LYALYISPVAMAPELVACIAAALYPSSRLHK"
     gene            9222..10304
                     /locus_tag="VMZ53_06325"
     CDS             9222..10304
                     /locus_tag="VMZ53_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28103.1"
                     /translation="MLDRRDLLKLALLVPACGKDFVEVCGGATTNAFDTVLDRLHTTD
                     MEFSGGLSNHGPMATEVLLHVGREDRICDWAGHYVPILNAAPAAGAPIAAADQVAQLG
                     NYDRRADWLATFLAGADLATVLPQLLPGMMALHGILRTGHALRAIAAASTPARQAELA
                     HGLAYWAARFERVPGDPGSQPEPARDLVTALDQLAIVPESKQVDGGLIKDRLVPLASL
                     PSFATDIARVDLDVWPIERSITELAAVAARLYIADAGRGDLGLLHAFDVTSILRVFLP
                     SLDAASQRAALGYAFQVVAALHATHSPRAGLPPGPYNAATTADAVAEIGADADDEHTI
                     KFTEALLREHAIDPRPEFLAAADLRI"
     gene            10371..10670
                     /locus_tag="VMZ53_06330"
     CDS             10371..10670
                     /locus_tag="VMZ53_06330"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUS28104.1"
                     /translation="MVRKQSNEDLYEVIFTALAHPARRRILHTLNFEGGSMSAGDIAA
                     MFSHAWPTTTRHLQVLESAGLVVSEQQGRARIYRIDKKRLELARDWISWFFKPPA"
     gene            10680..11081
                     /locus_tag="VMZ53_06335"
     CDS             10680..11081
                     /locus_tag="VMZ53_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28105.1"
                     /translation="MTTKQTNKTPSLFRINIEVGDIAQASKDWSTLLGQEGRSQAGAR
                     TYFTAGAVTLQVVQCDPPHPAAKALYFLVDELEPYHARAQQLGWLSKESVHGQPGGDI
                     SVRPWGERSFYVEDTWGNPLCFVQTGTTYPG"
     gene            complement(11083..12420)
                     /locus_tag="VMZ53_06340"
     CDS             complement(11083..12420)
                     /locus_tag="VMZ53_06340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUS28106.1"
                     /translation="MKTAILALVPLLGCVADAPGYQTVTDDSVGVTVELPSSYQVKKD
                     PVLFDTHGFLVLAPGDGDGDEVARIALAYQAKPADLESLVATKLDKYKDLNPTRTELT
                     LADGRAAVAVTGLPGTHGYAVVYVADGDRVYEIGLWSLDGTIDDRGRDILAHIKFEHP
                     ARTIQSLNLRRAEEALHDPAPTAIAARNAIAHTERVAAYEAAKQADPALAYAASHETT
                     LEPTRPRGPDGTAAAGCGFTAPSGMYWQLQWDGSNTFYSGSWYSLRGEPGWSAMSGNY
                     GSWWGTNFHVGLCYASYLNQYYANDWPTQYWGNAYAAFSGYVEWAGWGTDGFASLGRY
                     VVVRNGQYRSLTAHLLSIESGISWGAWIDGYSTIIGYAGDTGEYYAGADWAPHLHARV
                     AWGESLTWNGQPYGGQSVGPSALRSFHCTNCDVDAAGSGGWYTDFYHGRWMKY"
     gene            12612..16136
                     /locus_tag="VMZ53_06345"
     CDS             12612..16136
                     /locus_tag="VMZ53_06345"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28107.1"
                     /translation="MHPLAFAGTSRFEIRRRIGAGAMGVVYEAFDRERGMPVALKTMR
                     TETPASLARLKQELRALAGVTHPNLVTLHELHAVDGAWFLTMELTLGSDLATYIAGDE
                     TKLRECLGQLALAIQALHDAGHLHRDIKPANIVVTDAGRVVLLDFGVAGALGDRERVG
                     TPAYVAPEQTRGEACEASDWYAFGCVTYQLLTGALPFSGNTRAILYDKTHYAAQPVRA
                     RNANAPAELAALCDGLLRRIPTERPSGAEIVHLLRGTPRRRWPRASEGAPHLIGRDHA
                     LEIMRAALGDAEAGRATVVHVRGASGVGKTTLVQALLREASRDCVVLASRCYAQESVP
                     YKAVDSLVDALARFLSGLPEAEADAALPRDILLLAHVFPVLSSVDAIARAKRRRGPLP
                     EPHELRRRAFAALRELVARLAEQRTIVIAIDDAQWGDIDSAPILDGLVAPPDAPAVLL
                     VLAYRAADADDAPLLCALRQRRERLDGLATPIEIELEPLAHEDACTMAASVLGVPETD
                     PRCAAIATDARGLPFLIQELAHAVRGSGDALRAAVTSRLDSLPSAERMVLELTAVASR
                     PTARRVLGAASALPAAELETALARLVSERFVRVCADDARFECYHDRIREGVIARIAEA
                     ARRDHHMRLARALAIDPQREHEALAVHYLGAGDRARAGEHSLAAAEQASTALAFERAA
                     RLYRSALELADLDATNRREISAKLGDALANAGRGAEAGDAYLAAAAGAPRAAALELQR
                     RAASQYLRTGLIDRGLEVLDDVLRELAMKRPQTSKQAVLSLLWRRAQLRLRGMRMKVR
                     DASQVTPLELLRVDVVWTAATDLAMVDLIGGADFGTRQLLLALDAGEPVRAARGLLIE
                     AMFLAAQGGRTATRASSLIERAKELVATLDDAELPAWIDAAQGIVAYQAGDFRIAADR
                     ARTSVRRLREDCTGVSWEVGGVEVQEAWALFYLGELGRLARMVDAAVEHARVRGNRFD
                     AANFGTGLPALAWAVVDRTAQGRTEVEHAIAAWSQRGFHLQHYYSLLALASFDLYDGD
                     TVAARSRIARVWPSLERSHLLRCQSVAIEAHHLRGRAALAAGDREEAQRDVRALERYR
                     GNRWAAAMLALLRAGAEATSDALATTEATCTRTELTAFAAATRRRRGELDGDTALITE
                     ADRALLACGVQNPSHFARMLVPICERRR"
     gene            16133..17452
                     /locus_tag="VMZ53_06350"
     CDS             16133..17452
                     /locus_tag="VMZ53_06350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS28108.1"
                     /translation="MTETNLDALVTSPPTLGLPSETGTIRGIRIEVVAGPDAGLLHIA
                     AGDRVVVGTHRTADVVLTDRTVSRFHVELAIDRDGASLRDLGSRNGTLLDGVWIEKCR
                     LRGPTIVVVGSTELRIDLEGDLLTPTLAPQERFGDLVGASPVMRAVFARLEHAALHDT
                     HVLIEGERGTGKDLAAAHLHEQGARRDQPFDIVDCAGPPLDVEDALFGRRDDPGALVR
                     CDSGTLVLDEIGALSRTTQRTLARVLESGALRLARGDTQSVNVRIIALSRRSLRPDVN
                     ADRFAPDLYELVARARIRLPPLREHPEDVPLLVQWALDRVHGSSSRGAAHLLAADTLE
                     ALRCAPWPGNVRELRAYVEQTVAADGVSLDDNSEPPLVDATVTLRDARERWVRYFERE
                     YLTELLARSQGNVAAAAIIAGVDRVHLHRMITRCGLRDLLRSNREGH"
     gene            17662..18591
                     /locus_tag="VMZ53_06355"
     CDS             17662..18591
                     /locus_tag="VMZ53_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HUS28109.1"
                     /translation="MSGIDVSHWNGTIDWAAVRASGRRFAYIAIGDGTFVDPMFSTNW
                     TRARAAGLYRGVYQYFRASEDPVAQANIIVSRVGNLGPGDLPVMLDLEELDGMSAATV
                     TANVRAWLTAVQRGTGRLPIIYTNSSTFDFGMGAPRGFAGYPLFVANWQVSCPNIPNT
                     WASWKAWQWSATGRVPGISGDVDLDVFNGGLAQLASYAEQGADCAFGDGLYCGGNGVK
                     GDPRSLFKCSGGQKTLAKVCAEGCVVKPAGDDDACAPATFCPAGDGPYCGGDLIGGDR
                     NKLYRCTAGAVTEEQTCTNGCTWAPFGSDDHCT"
     gene            complement(18579..>19059)
                     /locus_tag="VMZ53_06360"
     CDS             complement(18579..>19059)
                     /locus_tag="VMZ53_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28110.1"
                     /translation="TAYRIRWANGGGTAYPVYEVNGLPDKSRDHAGAYGYQTGFSASY
                     VDPYLAQGGLRLDGSDFVDIELSTVGVPQIANATLAIYGRSYNTTASGSYNWQTFDGT
                     GATATNFVSNAAPYAWYRADMTTEISPNDDGVLLRIKAGPSSGSLVVNRIELCLQVQ"
BASE COUNT         3208 a         6340 c         6256 g         3255 t
ORIGIN      
        1 gccgcggctg aatgcgaagc tgcagcgtct gctcggcggt tcctcgcttg atcacgatcg
       61 tggccgtcga gccggctgcg tgctgctgga ccagcggcgg gacttctttc ccgatcgtga
      121 tcggatggcc gtcgaatgtg atgacgacgt cgtcgcggcg aatgcctgcg tcgtacgcgg
      181 gcgagcccgg aatcacctgg cccacgcgcg cggtccctgc gtcgaacacg acgccgatcc
      241 atttgggttg gccgtcgtcc ggcgcgggtg ctgcggcggg tgccggctgc gcgggtggtg
      301 cagcgacctt cgatgccgcc ggtgtgcacg cggcgaccgc gacgagcagc gcggccctca
      361 cagattgacc gaccgcatcg ccgcttcggg atagcgcatt ccagacgcga catccttcgg
      421 cgcgatccga tcgatttcgg cgagctcgtc ggcggtgagc cggatcgcat cggcggcgac
      481 gttctcctcg agatatgcgc gtcgcttcgt ccccggaatc ggcacgatgt cgtcgccgcg
      541 ggagaggacc cacgcgagtg cgagctgacc gggcgtgacg cccttctgct gcgcgaggcg
      601 cgtgatgtga tcgacgagct cgatgttctt cgcgaagttc tcgccctgga atcgaggcat
      661 gccgcggcgc cagtcgtcgg ccgcgaggtc ctccggcttc ttgatctggc ccgtgagaaa
      721 gccacgcccg agcgggctat acgcgacgaa gccgatgccg agctcgcggc acagtgcgag
      781 gagaccgtcc tccggctcgc gcgaccacag cgagtactcg gtctgcagcg cggcgatcgg
      841 gtggactttt gcggcgcggc gcagcgtctc ggggccggct tcggacatcc cgatgtagcg
      901 aatcttcccc gcgcgcacga ggtcggccat cgcgccgatg gtgtcctcga tcggcgtgct
      961 cgcatcgacg cgatgctgat agtagaggtc gatgtgatcc atgccgaggc gcgacaggct
     1021 cgcgtcgcac gcgctcttca cgtacgcggg tgagccatcg acgcgccgct tcgtcgggtc
     1081 gacactgccg acgatgccga acttcgtcgc gagcacgacc ttgtcgcgct tgctgccctt
     1141 cagcgctttg ccgagcagct gctcgttcgt gtgcggtccg tacatgtcgc tcgtatcgag
     1201 cagcgtgaca ccgagctcga tggcgcgatc gatcgtcgca agcgattccg cgtcgtcgag
     1261 cggaccgtag aacgccgaca tgcccatgca gccgaggcca atcgcgccaa ccgacgggcc
     1321 gttgcggccg agcttgcgag tttgcatggc ccgaaccgta gtacatgttc agcgatgtgg
     1381 cgcgtcccga cggcgctcga aacagagcca ttccggcatc tgatcatcga tgacgcgtgc
     1441 gagcgtcccg acgacctgct cgcgatcgtc gacgacgaag gcgtcgagcc gtacgagggc
     1501 gacatctttc gcttcgaggc caccactccc gagagccgcg ggctgcactc gatccgcgag
     1561 cagtttgccg cggagctcgc gcctgcgctg tcgcgactga ctggacgcac ggtgcgccgc
     1621 gtcgatatgc gcgcgtatgc gtatcgcccc gggcactact tgttgccgca caccgatcac
     1681 cgcgagggac tcgagcgcgt gctcgcgtac gcgtactact tgccaacacc ggagcctccg
     1741 aaaggtggcg agctcgagct gttcgcgtgc acgctcgcgg atgacgagct gacgtcgacg
     1801 atcagcgcca aggtgatcga gccgcgcgcg aatcggctcg tcatcttcga ggtcggcgac
     1861 acgtcgctcc atcaggtgcg cgaagtgctc gcgggcatgc gcctctcgct cgcgggttgg
     1921 ttctatgcct gagctgccga ggctcgaaga cctgttcggg accgcgctcg tgatccccga
     1981 cgcgattcca cgcggacagc tcgcgatggc gcgcgagcgg ctcaccgggt ggcagcgcta
     2041 tgccctgctc gatcgaggca gctacgagtg gatcgatgat gtcgacgtgc tgcgtcgcga
     2101 gatcgtcgag ctggccgagc gcgtaacgtc gcggacgggt ctcgcgattc gcgaatgccg
     2161 cgcactgcga ctcgtcgcgg gagactacct gctcgcgcat cacgatcgat tgcacgacga
     2221 caatcccgtc gaagtgatgg tcgacttgtc ccccgcgccc gcgtctgccg aagtccacta
     2281 ccgccgcaga ggccagacgt tcctacgcgt gccgtcggtt ccaggcaccg cggccgtcgt
     2341 cgagcgcggc ccttcgatca cgtgcaacca cacctacgtc agtaagcgcg atcccgcaac
     2401 gtgcgcgatc cgcctcgtcg cgctgctgaa gtaggatgcg gccatggcca cgacgaagcg
     2461 cgcggacttc ggaaaacccg tcgacggtta catcaagaag cgatcgcccg atcagcagaa
     2521 gatcctgacg gagctcaaga agctcatcac ggccgcggcg cccgagtgcg aggcccagat
     2581 caaatgggga atgccgcact acacgatcgg cggcaagtac tacgcggcga cgcccgcgca
     2641 caaagcgcac gtgaatctga tcatgttcgg gccggcaaag agcttcgatg atccggacgg
     2701 cctgctcgag ggcgaaggca aagtcggccg gcacctcaag atcgcgacgg cgaaagagct
     2761 gccgaaggcc aagatcaaga agtggctcgc gattgccgcg aaagcgtcgc gcagcgctta
     2821 ggcatcacgc acagacggac tcgctagaag ccgagcacgc tctgtgtcca gtccgcgccc
     2881 cacagctcgc gcgcgacgac gcgaagtggc tccgcggccg gcgatgaccg cagcgtcacc
     2941 gcgtactgtt cctgtgtctg ccacgagaac tcgttcatca ggtggccggt ctcgtagagc
     3001 ttcatcgcgc gcttgatgtt gtacgccgtg aagcagcgaa actgcgtgtt cgtcgtgtag
     3061 tacgccgggt ccttgtcttt cgccgttgca gcggtcgcca gcgcgtagac ggtgaactcc
     3121 agcgggccca tcacggcgtc ggtccactga ccgtcgtaga ggccagcctg cacttggcca
     3181 acgccgacct cggcgccgtt cacgtacgca ttccactcgt cgaagacgta gagcggcgtg
     3241 tcatcccatt cggtctgctg ctcgaggtag aggtgaaagc gcgggccgcg caacaccatt
     3301 gcgacgcgcg gcttgatgtc ggacttgcgc atgtccggct cggcgacgat cgcgaccttg
     3361 ccgttgagga cgtagaacgc gttgtagccg atgggctgcg gcgcgacgta gttgcgcagg
     3421 tgcgcatgga tcccgtgtga cgtctcgtgg cccgccgtga tcgcgtcgtc cgaccaatac
     3481 tggtcgccgt actgcgctgg caggtgacgc gcgatatccg tcagctgcgt gccccagctc
     3541 ggctggccga ggctcggttg ctgctcgggc cacgtcgtcc attgcacgca cgccgttgga
     3601 tcgtcggcgg ccggcgcgtc tggctcgacg acggcagcgt ccgactccga cgacgacgac
     3661 gcgtctggcg tctccgtcga cgtcgactcg tgaccacacc ccaaaacgac caagacgcac
     3721 gcagcgaatc ggcgcatgct gccatttcgc acgttgctga ctttgcgtca acaagcattt
     3781 ccggttaaca cgcttcagga tgttgctcgt gcagcgcgtg tcgcgtggcg cgtgctgcga
     3841 gccaggtcgc ggcgatcgtc gtggcgaggc cgccccacag cgcgacgtgc ttccacacgc
     3901 tcggcggctg cgcattcgac agctgcgtca cgctcgacaa gtacgagccg acgtacgtca
     3961 gcatcagcgt gcagggcagc atgccgacga agttcgtgac gagccgcttc cagagcggga
     4021 ccttcgtcat cgcgagcgcg tagttcagca cgccgaacgg catgagcggc gacaggcgca
     4081 gcaagaacaa gatcgagagc gagttcttgc gaatcgcagt atcgagcgcc tgcagtttcg
     4141 ggtggcgttc gatcatcggc tcgatgcgtt tgcgcaggct cgtcttgacg atgagcagcg
     4201 tgcacagctc gacgaccagg ccgagcgagt tcatgagcaa cgcgccccaa atggcgccgt
     4261 acaacatgcc ggccgcgaga tacatctcca tcgtcggcag gatcgtgacg gcgagcgcga
     4321 tgaagacgag tgagaagacg acgattccga gcacgccggt gccgcgcacc cacgtgacgg
     4381 cagcgcgaat ccactcgccg gcaggcagcc acaagacagc agccacgatg gccgcgccgg
     4441 cgacaacgag aagcgcgatc ttcatcgctc tgctcacgta cagagcttgt catggcgaat
     4501 ggaattgcat agttcccgca cagcgtgcgg gagcgctcgc agtgttaccg tgatcccgtg
     4561 aatcgtcgac ggtacatcgt cggtggtgca gcgctcatcg ctgcgattgc cgtcgttgca
     4621 gcgatcgcac agcggcaacg cacggtcacg agcgcgacac acgacaacac cgcaccggtc
     4681 cgctcgctga agtccgtcgt gctcgcgccc acgagctcgg ttgcgggcgt gattcgcgat
     4741 accgcgggag cgtcgctcgc cggcgcgact gtatgcgcgc agacaaggga tcgctctatc
     4801 agctgtgcga cgagcaacgc cgacggcgcg tacgcgatcg atcacttgtt cgcagcgacc
     4861 tacgaggtct cggcagccgc gccgaaattc aggccgacca gctatcaccc cggcggcgat
     4921 ccactgcgca ctcgattcgc tctcggcgag ggcgagcgtc ggactggcat cgacatcgcg
     4981 ctggacggca ccgccgtgga gctccgcggg accgtcctcg acgtgagcgg cggccccata
     5041 gctcacgctc gcgttcgcgt cgcgcacgag aacgtcaaca tcaacgagtg gcggcactgg
     5101 tatcccgcaa ccgaaaccgc ggacgacgga acgttcacgc tgtgggtcgc cgctggcgag
     5161 cgccgcctcg aagcaagcgc cgacggttat gcgagcgtcg agcaagatgc gcaggcgccc
     5221 ggacgcgtcg agctgctgct gattccaggc ggctcgatcg ccggcactgt cgtcgatcac
     5281 gagggcaagc ccgtcgccga cgcgctcgtt gtcgcggact ggatcgacga gcacaagcgc
     5341 gctcgcagcg acgacacggg ccacttcgag atcacgggcc tcgtgccggc acgctacgac
     5401 gtctcggcgc acaccgcgaa tgcgtacggc acggccgtcg gtagtgtgct cgtcggtctc
     5461 gccgcgcacg tcgacggcat catcgtccgt gtcgacccgg cggcgcacat cgtcggccgc
     5521 gtagtcacga agccgccatc aacgctctgc cccgccgccg acctcgacgt cacgcttcac
     5581 gatccgttcg gcgacaatat cgagatgacc gtcgccgcgg acggcaccgt gcatgccgat
     5641 ggcgtaatgg cgagtaccta ctatgccaac gtccgatgcc gcggctacat cgcagccgat
     5701 cgctatccgc cggtcgtcat cgcggccgcg gatcaagagc tgacgtggaa cgtggcgccc
     5761 gggatcacgc tccacggtca cgtccggacg ccggatggag caggcatcga ggatgcgatc
     5821 gtcgaggtcc gcaatggatt ccacagctac gacgccacga gctcgcgtga tggctcctat
     5881 gagatcggcg gcatcaggcc gggtgtgtat cgcgtgtggg cgtcgtccga gtacgccgat
     5941 gtgaccaaca cggagatgac gctcgacagc aacgtcgaga aggacttcgt gctcggcgtg
     6001 cccgcgacga tccgaggcat cgtcgtcgat gcggataacc atccggtgag ctccgtcgag
     6061 attcgcgctg aaggaaaggg gctcgcagga cggctcgatg ccagccggcc cgatggaacg
     6121 ttcgagctgc gtctcgcacc cggcacgtat gacgtctacg ccgcggtcga ttggcggcac
     6181 ccgctcggta agaaggaacg cgtgaccgtt tccgtcgaca agcctgcatc ggttcgcctc
     6241 gtcgtgccgg cgcagaccgg gacgatcaaa ggtgatgtga gcgatgcgtt cggccgcccg
     6301 gtctccgatg cattcgtcta cgcgtacacg ccgtcgatgt tcggcaacga cgagttgcag
     6361 atcggctgga acgaacatcc aacgctgacc gcgccggacg gcacgttcac gattaccggt
     6421 ctcggccccg ggacatacac cgtgatcgcg cagcgccgag gtggcggctc gacacgcgtg
     6481 accgacatcg agcgaggctc gaaagtgagt ctcgtactcg ccaagaccgc atcgctctcg
     6541 ggccgcatct cgtataccga ccacacgcac ccatcgcaga tgaagctgac cgtgcgcgcc
     6601 gataccgccg cagatcggtt cttccgcgag cagcgcttct atcacacgga cggtgtgttc
     6661 acgctcgacg agcttccagc cggcaccgtc acgatcgcgg tcgagacacc cgatgggctt
     6721 ggcgtcgcat ccgtcttgct gaccgcgggc gagcaacgcc gcgatgtcgc gctgacggtc
     6781 gaccgttcgg tgacgatcac cggccgcgtc gtcgagcagc tcacgcacaa agggatggcg
     6841 agctgcttcg tgatcccgcg ttcgacgcgc gcattgctcg gcgcggtcgc ggactacatg
     6901 gacgtcacgc accagaccga cgcggatgga accttcaaga tgctcgcacc tcccggcgaa
     6961 gtggcgctcg agatttcatc gaccgatgag acagaccgcg agctctgcgc gccgcctgtt
     7021 cgattggacc tgcgcagctc gctcaacgtc ggcgatatcg cgctcgttcg cccgcgcacg
     7081 aagattccga gcaacgtcgg gttcacgctc tcgggcggca cagtcgatag cgtcgccccg
     7141 aagggacccg ctgcagctgc cggactgcgc gtcggtgatg tcgtcacgtc gatcgatggc
     7201 gtcggccccg catcgttcgt ggccgaatgt gggttcgcat tgatcgcggt tccacctcgc
     7261 acgacgctga cgctgacgct tgcgcgcggc gacacgatca agatcacgtc gggagaccaa
     7321 ccggccggcg acgagatcga aatcgacgag cagccttgac gctctcgcgt tcgtggctac
     7381 gtcgatgcgc atgcgactga gaaccatctc ggctttgctc gtcggcgcca ccgttgcggc
     7441 ggtgctcatt tcgtcacaca ccgagcatgg gcagctcgcg aaccaggcgc ggatcaaccg
     7501 caacgcgtcg tcggaggtgc tgaaattcca cgcggtcctg caggcgcgca tcgatatcca
     7561 gctctcgcag cccgacctcg gtactccgac gcaagcaccc gacatctggc gcctaccgcc
     7621 gatccagcgc cagcgcgtgg gctagccgat ctggtagctg cgcgctagct cgtcgcgaac
     7681 cacgtgccgc gtagcgtgcc ttcgatcaag ttggggagcg tgttgaacag gcgcaccgtc
     7741 ttgcgcgcgt tcgttcgata gatctgctcg cggatcgttg cccgtgccgc ttcatcgggt
     7801 gcactcgata gctgcaatgc gtggacgaac gtcttcgcgc gccaccggcg gatcatcgtc
     7861 atcgagattt cgccaacact cttcacgccc cccgattcga taatcttctt cgacatggga
     7921 cagtcgaact tcgccgcgag tagctcggtc gagcgctcga ctttctcggc gaggatcgta
     7981 tcgacgaagt tgtgaccgtt ctcgatctgc tcgagcgccg ccttgtcgtg tgcgaagcgg
     8041 agctcgcgaa tcgtttcgtc gagcgcgcac gcgagatcga agttgatgtg ctggcacatg
     8101 ccgacggtga agtgcgccat cgcaggcttg gtggcgagcg gcgtcgctgc atccggcgag
     8161 agattgtcga acgcgaagct cggacgccag agccggtagc gcgccgcgtc gctcgtcgta
     8221 tcgaggccga ggtcggcttt cagcccgtcg aagtggcgct ccgcgaagcg accggcgagt
     8281 cgcacgaaga atggcttcgg tgtcaggacg ccgcgaccga gcgcgtcgat caccgcgacg
     8341 gtgatgccgt gatagacgtc gaggaacgcc gctgcgcgat ggccatgctc gaggagcaga
     8401 tcggtcagct gcgtctgcgc ccggtctgcg gcttcgatcg tctgcggcgt cgtgtcgatg
     8461 tcgcgagcga gcgaggcgcg gagcttgtcg gcaagcggcg ccggaaactc cgtcgagccc
     8521 ggcaagaacg ccgagagcgg ctgcagatca ggcttcgccg cctcgtcgaa gatatccatc
     8581 gccgtcactc tacttgtgca gccggctgct tggatagagc gccgccgcga tgcacgcaac
     8641 gagctcgggc gccatcgcga cggggctgat gtacagcgcg tagaggatcg cgactgcgat
     8701 ggtgaggcga atgagatcgc ggccgcgcag cgtcaccatg ccgtacacga cgagcgcacg
     8761 ctgcgggaac tgccgcgccc acgcgatgac gagcgtgtcg accatcgccc acccgccaag
     8821 gcgaaacacg aagcggccgg acaccgccgc caccagacac gtaaccacgc ttgctgcgac
     8881 gacgacctcg atgatgaatc tgcgcaaccg ccggtcaccc cagcgcaccg cgagctcgcc
     8941 gccgaacttg tagatcgcga gacacgtgat gacgagcccg agcggcccag gctcgacgag
     9001 cggccacgta acaagccgcc atacctcgcc atgccagatg cgatgcggcg cgagcgcaaa
     9061 ccagcgtgcg agccacccgc catcgaagac agccacgagc gagcacgtca gcgtcgcgac
     9121 gatccattgc gtcgtgaagc tgctcgaccc gagcctcggc cacatccgtg catcgtacta
     9181 gctcttgcat ccgctcggcg tggtgtgccg acaatggcga gatgctcgac cgtcgtgatc
     9241 tcctgaagct ggcgctgctc gttcctgcgt gcggcaaaga cttcgtcgag gtctgcggcg
     9301 gcgcgacgac caacgcgttc gacaccgtgc tcgatcgcct ccacacgacg gacatggagt
     9361 tctcgggcgg cctgtcgaac catgggccga tggcgaccga ggttctgctc cacgtcgggc
     9421 gcgaggatcg catctgcgac tgggccggcc actatgtccc gatcttgaat gcagcaccgg
     9481 ccgcgggcgc gccgatcgct gccgcggatc aagtcgctca gctcggcaac tacgatcgcc
     9541 gcgcggactg gctcgccacg tttctcgccg gcgccgacct cgcgacggta ttgccgcagc
     9601 tgctgcccgg catgatggcg ctgcacggca tcctgcgaac gggccacgcg ctgcgcgcaa
     9661 tcgccgcggc ctcgacgcct gcacgccaag cggagctcgc tcatggtctc gcgtattggg
     9721 ccgcacggtt cgagcgcgta cccggcgatc ccggctcgca accggaaccg gctcgcgatc
     9781 tcgtcaccgc actcgaccag ctcgcgatcg tccccgagtc caagcaggtt gacggcggtc
     9841 tcatcaaaga tcggctcgtg ccgctcgcgt cgttgccgtc gttcgcgacg gacatcgcgc
     9901 gcgtcgacct cgacgtgtgg ccaatcgagc gctccatcac cgagcttgcc gctgtcgctg
     9961 cacggctcta catcgccgac gcgggccgcg gggacctcgg cctcctccac gcgttcgacg
    10021 tcacgagcat cttgcgcgtc ttccttccgt cgctcgatgc tgcctcgcaa cgcgccgcgc
    10081 tcggatacgc attccaggtc gtcgcggcgc ttcacgccac gcattcgcct cgcgcgggcc
    10141 tgccaccggg tccctacaac gctgcgacca ccgccgacgc ggtcgccgag atcggtgcag
    10201 acgccgacga cgaacacacg atcaagttca ccgaggcgct cctgcgcgaa cacgcgatcg
    10261 atccgcgccc tgaattcctg gcagcagcgg atttgcgcat ctagctacat atttagcgcc
    10321 gtggctaaat aacgctagac aacgtttagc gtcgcggcta aataacaggc gtggtacgaa
    10381 agcagagcaa cgaagacctc tacgaggtca tcttcactgc gctcgcccac cccgcgcgtc
    10441 gtcgcatcct ccacaccctc aatttcgagg gcggctcgat gagcgcgggc gacatcgccg
    10501 cgatgttctc ccacgcgtgg ccgacgacga ctcgccatct gcaggtgctc gagtccgctg
    10561 gtctcgtcgt gtcagagcag caaggccgcg cgcgcatcta ccgcatcgac aagaagcggc
    10621 tggagctcgc gcgcgattgg atctcgtggt ttttcaagcc gcccgcgtag gagacgatca
    10681 tgacaacgaa acagacgaac aagactccct ctctcttccg tatcaatatc gaggtcggtg
    10741 acatcgcgca ggcgtcgaag gattggtcga cgctgctcgg gcaagaaggg cgatcgcaag
    10801 cgggggcgcg cacctacttc acggcgggtg ccgtcacgct gcaagtggtg cagtgcgacc
    10861 cgccccatcc cgcggcaaag gcgctctact tcctcgtcga cgagctcgag ccgtatcacg
    10921 ctcgcgcaca gcagctcggc tggctttcga aggagtccgt gcacggccaa cccggcggcg
    10981 acatcagcgt gcgtccatgg ggcgagcggt cgttctacgt cgaagacacg tggggcaacc
    11041 cactctgctt cgtgcagacc ggcacgacgt accccggctg agtcagtact tcatccagcg
    11101 accatgatag aagtcggtgt accaaccgcc gctaccggcc gcatcgacgt cgcagttcgt
    11161 gcagtggaag ctgcggagcg cggacggacc gacggactga ccgccgtacg gctggccgtt
    11221 ccacgtcagc gactcacccc acgccacgcg tgcgtgcagg tgcggcgccc agtctgcgcc
    11281 cgcgtagtac tcgccggtgt cgccggcgta cccgatgatc gtggagtagc cgtcgatcca
    11341 cgcgccccac gaaatgccgc tctcgatcga gagtagatgc gcggtcaggc tgcggtactg
    11401 gccgttgcga acgacgacgt agcggccgag gctcgcgaac ccgtcggtgc cccatccggc
    11461 ccactcgacg tagcccgaga atgcggcgta cgcgttgccc cagtactgcg tcggccagtc
    11521 gttcgcgtag tactgattga ggtagctcgc gtagcagaga ccgacgtgaa agttggttcc
    11581 ccaccagctg ccgtagtttc cgctcatcgc gctccaaccc ggttcgccgc ggagcgagta
    11641 ccagcttccg ctgtagaacg tattgctgcc gtcccactgc agctgccagt acatcccgct
    11701 cggtgcggtg aagccgcagc cggccgccgc ggtgccgtcc gggcctcgag gacgtgtcgg
    11761 ctctagtgtt gtctcgtggc tggccgcgta cgcgagagca ggatcggctt gcttcgccgc
    11821 ttcgtacgcc gcgacgcgtt cggtgtgcgc gatcgcgttg cgtgcggcga tcgccgttgg
    11881 cgctggatcg tggagcgcct cctcggcgcg gcgaagattc aatgactgga tcgtgcgcgc
    11941 ggggtgctcg aacttgatgt gcgcgaggat gtcgcgcccg cgatcgtcga tcgttccgtc
    12001 gagcgaccac agcccgatct cgtagacgcg atcgccgtcg gcgacgtaga cgaccgcgta
    12061 tccgtgcgta ccaggcaggc ccgtcactgc gacggcggcg cggccgtccg caagcgtgag
    12121 ctcggtacgc gtcgggttga ggtccttgta cttgtcgagc ttcgtcgcga cgagggactc
    12181 gaggtcggcg ggctttgctt gatacgcgag tgcgatgcga gccacctcgt cgccgtcacc
    12241 atcacctggt gcgagcacga gaaagccgtg cgtatcgaac agcacggggt ctttcttcac
    12301 ctgataggag ctcggaagct cgacggtgac gccgacgctg tcgtctgtaa cggtctggta
    12361 acccggggcg tccgcgacgc aaccgagaag agggactagc gcgagtatgg ctgtcttcat
    12421 gctccgccgt tctgcaacgg ctacgccgac gtgaaacatg tggaagctat ggagacctca
    12481 ggctccagtt gtcgggtcgg ccccgcattt gattgggacc ctcacagtga tgtcgatggt
    12541 cactacgacg cggacagcga cggcaagtga cagtgagtcg gcgcgtgcga tgcgatggct
    12601 acgatgtgca cgtgcatccg ctcgccttcg cggggacgtc gcgattcgag atccgccgca
    12661 ggattggcgc gggtgccatg ggcgtcgtct acgaagcatt cgatcgagaa cgcgggatgc
    12721 ccgtcgcgct caagaccatg cgcaccgaga cacctgcttc actcgcgcga ctcaaacaag
    12781 agcttcgcgc gctcgccgga gtgacgcatc caaacctcgt aacgctgcac gagctgcatg
    12841 ccgtcgacgg cgcgtggttt ctcacgatgg agctgacgct cgggagcgat ctcgcgacgt
    12901 acatcgcagg cgatgagacc aagctgcgcg agtgcctcgg ccagctcgca ctggcgatcc
    12961 aggcacttca cgacgccggg catctgcatc gcgacatcaa gccggcgaac atcgtagtca
    13021 ccgatgcagg tcgcgtcgtg ctcctcgact tcggggttgc aggtgcgctc ggcgatcgag
    13081 agcgtgtcgg cacgccggcc tatgtcgcgc cggagcagac gcgcggtgag gcatgcgagg
    13141 cgagcgactg gtacgcattc ggctgcgtca cgtaccagct cctgacgggt gcactgccat
    13201 tctcgggaaa cacacgcgcg attctctacg acaagaccca ctacgccgcg cagcccgtcc
    13261 gcgcgcgcaa cgccaatgcg cctgcagagc tcgcagcgct ttgcgacggg ctgctccgac
    13321 gtatcccgac cgagcggcca agcggcgcag agatcgtgca cctgttgcga ggcacgcccc
    13381 gccgccgatg gccgcgagcg agcgagggcg ctccgcattt gatcggacgc gaccacgctc
    13441 tcgagatcat gcgtgcggcg ctcggcgatg ccgaagcggg acgcgcaacg gtcgtccacg
    13501 tgcgtggcgc ctccggcgtc ggcaagacga cgctcgtgca ggcgctgctg cgcgaagcga
    13561 gtcgcgactg cgtcgtgctt gcaagccgat gttacgcgca agagtcggta ccgtacaaag
    13621 cggtcgatag tctcgtcgac gcgctcgcgc ggtttctctc cggcttgcct gaggccgagg
    13681 ccgacgccgc gctgccacga gacatcttgc ttctcgcgca tgtcttcccg gtgctctcga
    13741 gcgtcgacgc gatcgcgcgt gcgaaacgcc gccgcggtcc gttgccagag ccccacgagc
    13801 tcaggcggcg ggcgttcgct gcactgcgcg agctcgtcgc caggctcgcg gaacaacgca
    13861 cgatcgtgat cgcgatcgac gacgcccagt ggggcgacat cgacagtgcg ccgattctcg
    13921 atgggctcgt tgcgccaccc gatgcgcctg ccgtcctcct cgtgctcgcg taccgagccg
    13981 cggatgcgga cgatgcgccc cttctgtgcg cgttgcgcca acgccgcgaa cgcctcgacg
    14041 gcctcgcgac accaatcgag atcgagctcg agccgctcgc gcacgaagat gcatgcacga
    14101 tggcagcaag cgtgctcggt gttccagaga cagatccgcg gtgtgcggcg attgcgaccg
    14161 atgcgcgcgg cctacccttc ctgattcaag agctcgcgca cgccgtgcga ggcagtggtg
    14221 atgcactgcg tgccgccgtc acgtcgcgac tcgattcgct tccgagcgcg gagcgcatgg
    14281 tactggagct gactgcagtt gcgagccgac ccacagctcg ccgtgtgctt ggcgccgcga
    14341 gcgcactccc cgccgccgag ctcgagacgg ctcttgcgcg tctcgtgagc gaacgcttcg
    14401 ttcgcgtctg tgccgacgac gcccggttcg agtgctatca cgatcgcatt cgcgagggcg
    14461 tgatcgcgcg aattgcggag gccgcccggc gcgaccacca catgcggctc gcgcgcgcat
    14521 tagcgatcga cccgcaacgc gagcacgagg cgttggcggt gcactatctc ggtgcgggcg
    14581 atcgtgcgag ggcgggggag cattcgctgg cagccgcgga gcaagcgagc acggcgctcg
    14641 cgttcgagcg tgcggcgcgg ttgtatcgct ccgcgctgga gcttgcggat ctcgatgcga
    14701 ctaaccgccg cgagatctca gcgaagctgg gcgatgcgct cgcgaacgcc ggacgcggtg
    14761 ctgaagcggg cgacgcatat ctcgctgcag ccgccggcgc tccgcgcgct gccgctctcg
    14821 agctccagcg tcgggcagcg agtcagtact tgcggaccgg gctcatcgat cgcggcctcg
    14881 aagtcctcga cgacgtgctg cgcgagctcg cgatgaagcg cccgcaaacg agtaagcaag
    14941 cggtgctgtc actgttgtgg agacgcgcgc agctgcgact gcgcggcatg cggatgaagg
    15001 tccgcgatgc atcgcaggta acgccgctcg agcttcttcg ggtcgacgtc gtctggaccg
    15061 cagcgaccga cctggcgatg gtggacctca tcggcggtgc tgacttcggc acgcgccagc
    15121 tgttgctcgc actggatgcc ggtgagccgg ttcgtgccgc acgggggctt ctcatcgagg
    15181 cgatgtttct cgccgcgcaa ggcggacgca ctgcgacgcg agcgtcatcg ctgatcgagc
    15241 gcgctaaaga gctcgtagcc acgctcgacg acgccgagct gcctgcgtgg atcgatgcgg
    15301 cgcagggaat tgtcgcgtat caggcaggcg acttcaggat cgccgcggac cgagcacgga
    15361 cctcggttcg tcggctgcgc gaggactgca cgggcgtttc ctgggaagtc ggcggcgtcg
    15421 aggtgcagga ggcctgggcg ctgttctatc tcggtgagct cgggcggctc gcccgcatgg
    15481 tggacgcggc cgtcgagcac gctcgcgtgc gtggcaatcg cttcgatgca gcgaacttcg
    15541 ggacgggctt accagcgctc gcgtgggctg tggtcgaccg caccgcccaa ggccgcaccg
    15601 aagtcgagca cgcgatcgcg gcgtggtccc agcgcggatt tcatctccag cactactact
    15661 ccctgctcgc gctcgcgagc ttcgatctat acgacggaga taccgtcgct gcgcgttcac
    15721 gcatcgctcg ggtctggccg tcactcgagc gctcgcacct gctccgctgt cagtccgtcg
    15781 cgatcgaggc tcaccatctg cgtggacgcg ctgcactcgc cgcgggcgat cgcgaagagg
    15841 cgcaacgtga cgtgcgcgcg ctcgagcgct atcgaggaaa ccgatgggcg gccgcaatgc
    15901 tcgcgttact gcgcgccggt gcggaagcta cgtcggacgc gcttgccacc acggaagcga
    15961 catgcacgcg caccgagctg acggcgtttg cagctgcgac tcgccgtcgt cgtggcgagc
    16021 tcgacggcga caccgcgctc atcacagaag ccgatcgcgc gctcctcgcg tgcggcgtgc
    16081 agaatccatc ccacttcgca cgcatgctcg tgccgatttg cgagagacgc cgatgacgga
    16141 gacgaacctc gacgcgctcg ttacgtcccc accgacgctt ggcttgccgt ccgagacagg
    16201 cacgattcgc ggcatccgca tcgaggtcgt tgcaggtccc gatgccgggc tcttgcacat
    16261 cgcggctggc gatcgcgtcg tcgtcggcac gcaccgcacc gcggacgtcg tcctgacgga
    16321 ccgtacggtg tcgcgctttc acgtcgagct cgcgatcgat cgtgatggtg catcgctgcg
    16381 cgatctcggc agccgcaacg gcacgctgct ggacggcgtt tggatcgaga agtgtcgctt
    16441 gcgcggaccc acgatcgtcg ttgtgggctc gacggagctg cgaatcgatc tcgaaggcga
    16501 cctccttacg cccacacttg cgccgcaaga gcgattcggc gacctcgtcg gcgcgtcgcc
    16561 cgtcatgcgc gcggtgtttg cgcgtctcga gcatgcggcg ctccacgaca cgcatgtcct
    16621 catcgagggc gagcgcggaa ccggcaaaga cctggccgca gcgcacttgc acgagcaagg
    16681 agctcgccgc gatcagccat tcgacatcgt cgattgtgcg ggcccgccct tggacgtcga
    16741 agatgcgctc tttggtcgtc gcgacgaccc tggcgcgctc gtccgctgtg acagcggcac
    16801 gctcgtcctc gacgagatcg gcgcgctttc tcggacgacg cagcgaacac ttgctcgcgt
    16861 gctcgagtcc ggcgcgctcc gactcgctcg cggcgatacg cagtccgtca acgtccgcat
    16921 catcgcgctc tcgcgccgaa gcctgagacc ggacgtcaac gcagatcgct tcgcaccaga
    16981 tctctacgag ctcgtcgcgc gcgcacgcat tcgcttgccg ccgctgcgag agcaccccga
    17041 agacgttccg ttgctcgtgc aatgggcgct cgatcgagtg cacgggtcgt catcgcgcgg
    17101 ggccgctcat ctactggccg ccgacactct ggaggcactt cgctgcgcgc cttggcccgg
    17161 caatgtccgc gagctgcgag cgtatgtcga gcaaaccgtt gctgccgacg gcgtgtcgct
    17221 cgacgacaac tcggagccac cgctcgtcga tgccacggtc acgttgcggg atgcgcgcga
    17281 gcgctgggtc cgctacttcg aacgcgagta tctcaccgag ctcctcgcgc gttcgcaggg
    17341 caacgtcgcc gcggctgcca tcatcgccgg cgtcgatcgc gttcatctcc accgaatgat
    17401 cacgaggtgc ggtctgcgcg atctactccg cagtaaccgc gaaggtcact agggcagctc
    17461 ccatcagagc tcacatcgaa tccgtgatat cgcccggttg tgtgcgcaca cgcgttgcag
    17521 tggtcgcacc gcatgaaact tactgctctc gtgttccttg ctgtcgcggc atgcgcagat
    17581 gccgggtcag cctccaccga agatgaaacg gcggtctccg ctagcgagca aagtctcgtg
    17641 tcatgcgcga cgggcgggat ggtgagtggc atcgacgtgt cgcactggaa cggcacgatc
    17701 gactgggctg cagttcgcgc atcggggcgg cgcttcgcgt acatcgcgat cggagacggc
    17761 acgttcgtcg atcccatgtt ctcgacgaac tggacgcgcg cgagggccgc cggcctctat
    17821 cgcggcgtgt accagtactt ccgggcgagc gaagatccgg tcgcgcaggc gaacatcatc
    17881 gtcagccgcg tcggcaatct cggccccggc gatctcccag tcatgctcga tctcgaagag
    17941 ctcgacggca tgagcgcggc gaccgtcacg gcaaacgtcc gcgcgtggct caccgccgtg
    18001 cagcggggca ccggcaggct tcccatcatc tacacgaaca gcagcacgtt cgacttcggc
    18061 atgggggccc cgcgcgggtt tgccggctac ccgctgttcg ttgcgaactg gcaggtgagc
    18121 tgtccgaaca ttccgaacac gtgggcgagc tggaaggcct ggcagtggtc agcgacgggc
    18181 cgggttccgg gtatttctgg cgatgtcgac ctcgacgtct tcaacggtgg cctcgcgcaa
    18241 ctcgcgtcat atgcagagca aggcgccgac tgcgcgtttg gcgacggcct gtactgcggc
    18301 gggaacggcg tcaaaggcga tccgcgctcg ctgttcaaat gcagcggcgg ccagaagacg
    18361 ctcgcgaagg tctgcgcgga gggctgcgtc gtgaagcccg cgggcgatga cgatgcctgc
    18421 gcgcccgcta cgttctgccc tgcaggtgat ggtccctatt gcggcggcga tctcatcggc
    18481 ggcgatcgga acaagctcta tcgctgcacc gcaggtgcgg tcacggagga gcagacgtgc
    18541 acgaatggat gtacgtgggc gccgttcggt tccgacgatc actgcacttg aaggcagagc
    18601 tcgatgcgat tgacgacaag cgagcccgac gacggacctg ctttgatgcg aagcagcacg
    18661 ccgtcgtcgt tcggcgaaat ctcggtggtc atgtcggcgc gataccaagc gtacggcgcg
    18721 gcgttgctga cgaagttcgt cgccgtggcg ccggtgccgt cgaacgtctg ccagttgtag
    18781 cttccgctcg cggtggtgtt gtagctgcgg ccgtagatcg cgagtgtcgc gttcgcgatc
    18841 tgcggcacgc cgaccgtcga gagctcgatg tcgacgaagt cgctgccgtc gagtcgcagc
    18901 ccaccttgcg cgagatacgg atcgacgtac gaggcggaga agccggtctg atagccgtac
    18961 gcgcccgcgt gatcgcgcga cttgtcgggc aagccgttca cttcgtagac gggataggcc
    19021 gtgcccccgc cgttcgccca gcgaatgcgg tacgccgtc
//
LOCUS       DATLBZ010000119         9962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9747495, whole genome shotgun sequence.
ACCESSION   DATLBZ010000119 DATLBZ010000000
VERSION     DATLBZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9747495"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VMZ53_06365"
     CDS             <1..760
                     /locus_tag="VMZ53_06365"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS28111.1"
                     /translation="PAPLATSAIPVEYQDIDILIPDFTPPQKVTSTAPGTAKPVAPNQ
                     TPIPIVRRQTIRPAITTGNDVDPAKLAGLFTNDDAAARRPGMDLGKQIIDARNNHVEV
                     GKDSAPDPSPRLGTQPDLPLTDPTLTHVDPTHGNEPKGRIIPGTIKEQAKTTLTPSLV
                     LQLIQSQYIRGLQRCYQRGLATEGASLAGKVSIEFTVEESGKVSDSSASGMSSTVDTC
                     IEGQMSTWRFPAPHDAQGAATEASFSITLALQPS"
     gene            777..1625
                     /locus_tag="VMZ53_06370"
     CDS             777..1625
                     /locus_tag="VMZ53_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS28112.1"
                     /translation="MRSAAVVLVALTAVASAKPARWQTLTPPTATMPTAASTGFVERD
                     GASLYYATYGDAKNPPVVLLHGGMGNSDHWVLQIPALTDKLYVIAIDSRGQGRSTRTK
                     AKPSYDLMADDVLAVLDKLEVKHAAFVGWSDGGEIALKLGIHHPDRVDKLFVMGANYD
                     ANGSKPRSGKPSQTFATYSARCRKDYAKLSKTPKQFDQLTQWLLPIWKNPMGFTKDQL
                     KQIKAPTVIADGDHDEIIKLSQLEEMAKLIPNAKLRVFADTSHFVLWQDTESLNAALV
                     EFLTAN"
     gene            1680..2765
                     /locus_tag="VMZ53_06375"
     CDS             1680..2765
                     /locus_tag="VMZ53_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28113.1"
                     /translation="MRSLRLAAVPFVLFACGDNHDQQMPVDANRPDTMVDAPPVPTCE
                     FNEANDAGNDDLFGTGTPEATNIAFAATKMTVCGSLNTGHFDTNSLLIDSDSYTVHVP
                     TSRGARVTLTGPGAEALNTVLIEIYDNVSGTDAVGTFLGNHAAAGVDLVAGDYTITVS
                     AFHTTAPAAAIPYRLTLWPVDLAMACPHVTTTASYTEANDLVTADGNDVYEVRYGSAT
                     PHRKFTDNALDAPENTTTAFTIAPAMNYRVTGVNSSPTVTPADWADRYQDRDTYAIKT
                     GANTNEVSLRLNWPGTTMDMDMLVFKENDLTDFADAFLNGNMEPEFLTIAVQPNSTYW
                     VFVAADDASTGQPVNYDLTVCGATFAP"
     gene            2767..5037
                     /locus_tag="VMZ53_06380"
     CDS             2767..5037
                     /locus_tag="VMZ53_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28114.1"
                     /translation="MPQSGDLIGPYRLVSQLGKGAMGEVWRARDERLDRYVALKVLPA
                     ELAGDAERRARMLREARAAAAIRHPNVVTLFDIVENKDDGDSLVMELVEGRTLADVLR
                     KEAAPPLEQALRWIEGVADALVAAHARRILHRDIKAANVMVTTDGGIKVLDFGLAKLR
                     DDASPSGSGNMIPASASLAAKIALDETQASQQGDSYQTHAGSLLGTPLYMAPEQIAGS
                     APDERSEVFSVGVLAYEILRGKPPYTAGTLEDLFRQITSEAPPPLEGIPDPIASVVHQ
                     ALEKEPTARWESMRAFRDAIAVERKRRFARSAPRWPVVAAALVLLVLAVGGTALWWTR
                     SHRAPPPRPGDVYVTRALDEYNLFYNDKALSSLRAALREIPEHPRANAYMLLFGGAPP
                     EDLEAALAGAKRALPKTALHSKDRALVEAAIAYSDRGAAEAKVALLGAGAAQDRELAF
                     WAAALDWRSGNFQAAHAGFQALLAQPARQFRGRIYDLESSILIYLDEPAEALRIGTLY
                     RDAFPGEADAVAVYATTLAVNGRYDEALVAAEDARRLNEGEDTLAGLAKVHALRGERA
                     KAKELYQQSLERAGAARRPIRRAALAVLQWADGELDAAKETVKPCLSGGDATARERGQ
                     CLFVAGIIEPERAEAIATKLDELAAESTPTRPSYGFPRSLATLLRARAKFFGGACLVD
                     VPLPSASIDEASLDVPLDFYAAYHVPFLATWSICERAALRAAKGDRAGAAALLQPVAN
                     RAPNRTWLLKALQRYQ"
     gene            complement(5012..7306)
                     /locus_tag="VMZ53_06385"
     CDS             complement(5012..7306)
                     /locus_tag="VMZ53_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HUS28115.1"
                     /translation="MRARDWVIAVSLLVAVALSILWMGGVLRTAQALVAAVVAMGVGA
                     TALSQRGFSRVSPLVALIAAAAALTALQLLPLGRAIGDRVTPTTSSLRSDGADLLGTS
                     PWTGASSDAAATLGALIVFLALLGIAMLALRIATSERGRYRLVAGVAVLCGVTAIVHG
                     VHTLFELDAVYGIYKPQDAHPQVFGPVLNNNTLACLMAAGACLGIALAAHRKQPGWLR
                     AMWLLLVPLCGAIVVATVSRGGTIALVAGAFITVGTLVAQRLTGADRSRKRRARFLSS
                     ALPIGILAGCVTVLVIWSNATNVERQFSQLSLDEVHFSRSKFVAWRSAGQLIHENVWL
                     GVGRGAFEAPFTRVHEASGLATYPYLENEYLQAVVDFGVPGALILAAIAFWFVWLAIR
                     RWRDNALSAGVLGALTVVAAQSNVDFGLEFLGLAAPVTALAATVLYVPLREAVRPRLA
                     RGLRVVHAVALLAGAALLMSSVTTTLDEDRQDLSKHATMALVRESATRHPLDYYAYAV
                     GAELLAAKKDPRAIRLLNHAMALHPTHPQLHRMAGRMLLADKFNSQAAIEYAAALRMT
                     EQPKPLLSEILEKFPGETAASALPLEYSDLRLVVTTLEDLKRADVARIWLARVLDVRP
                     KTAQPCELMFMLAEHGDLLAADIAGRRCSDRLPDFQARAGLAKMLVEKKAYREAIQLL
                     TDVESWQARRDEKIDAWLVMCDAERDLGQLDEAKRCLRRLDASPDMRNERRGEILTRI
                     ETINRAKAPPLPGTIPATGSAATP"
     gene            complement(7306..7890)
                     /locus_tag="VMZ53_06390"
     CDS             complement(7306..7890)
                     /locus_tag="VMZ53_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HUS28116.1"
                     /translation="MRLLVLAFLAFVACGDNPPVVYPTVAPFDPAKLALGPGDKLELT
                     VFYGQNEQKQTYTLDASGQMEVRYIGAVSANGKTTHEVQEEIRSRLADGYLNEPIVQL
                     QVVEINSLKCQVLGQVSKSGTVKFTPGMTIVEAIAQSGGFTPLARKNMVKVTRTVDGK
                     KETYKIPVEMIAEGKRPNFPMMPGDEIFVPERAW"
     gene            8144..8566
                     /locus_tag="VMZ53_06395"
     CDS             8144..8566
                     /locus_tag="VMZ53_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28117.1"
                     /translation="MQSATIKKQIDDDLRHVLHLQALARKSKDVIKLTCINDKLVQIK
                     AQLNLYDNANLQLETSINNGDGTASTSFSDVENTGADVKKLRGEADGCAGELELYKQE
                     SATNVDRPPDLDDPTETGGFEPPEIPELEPPGYATPFK"
     gene            8775..>9962
                     /locus_tag="VMZ53_06400"
     CDS             8775..>9962
                     /locus_tag="VMZ53_06400"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HUS28118.1"
                     /translation="MNRTVQVAIDAVILSLAYWLAFLFRLEFAISATWFRVLLVTWPY
                     VVILQYASLSAFGVPRLSWRYISILDVTRVLSAVTTANAVLLVIRLMGPLIGPHLELV
                     VIPIGVIAMNFVLAFLGLVGIRTVRRMQGEASDRKQKEAGAERQRVLLIGAGQAGVMV
                     AREIMNRPDLGLHPVGFLDDDPSKLRTSIGGLPVLGTTAQIREVAERKHVRRALITIA
                     NAPGNHIRRITELCRDAELETKIIPGIYEIVGAKVNLSRIREVAIEDLLGREPVQLDE
                     DVVGASIRSRVVLVTGAGGSIGSELCRQVSRFGPSRLVLVERYENALFEIHRELTQAF
                     PHVPIEPCIADVCDATRMEQVFTATKPELVFHAAAHKHVPMMEWNPCEAVKNNIGGTR
                     VLGD"
BASE COUNT         1934 a         3343 c         3046 g         1639 t
ORIGIN      
        1 gcccgcgccg ctcgcgacct ccgcgatccc ggtcgaatac caagacatcg acatcctcat
       61 ccccgacttc acgccgccgc aaaaggtgac gagcacagca ccgggcaccg cgaagccggt
      121 cgcgccgaat cagacgccaa ttccgatcgt acgtcgccag acgatccgtc ctgcaatcac
      181 caccggcaac gacgtcgatc cggcaaagct cgcaggcctc ttcacgaatg acgatgccgc
      241 tgctcgccgc ccaggcatgg atctcggcaa gcagatcatc gacgcacgca acaatcacgt
      301 cgaagtcggc aaggactcgg cgccagatcc cagcccgcgt ctcggcacgc aacccgacct
      361 gccgctcacc gatccgacgc tgacgcatgt cgatccaacg catggcaacg aaccaaaggg
      421 ccgcatcatc ccgggcacca tcaaggagca ggcaaagacg acactcacgc cgtccctcgt
      481 gctgcagctg atccagagcc aatacatccg cggcctgcag cgctgctatc agcggggcct
      541 cgcaacggaa ggcgcaagcc tcgccggcaa agtctcgatc gaattcaccg tcgaggaatc
      601 tggcaaagtg tccgactcgt ccgcgtcggg catgtcgtcg accgtcgaca cctgcatcga
      661 gggccagatg agcacctggc gcttcccggc tccgcacgat gcgcaaggcg ccgccacgga
      721 ggcctcgttc tccatcacgc tcgccctcca gccgagttga tctacaatcg ctagcgatgc
      781 gttccgcggc tgtcgttctc gtcgcgttga cggccgtcgc gtccgcaaag ccagcacgct
      841 ggcaaacgct cacgccgccg actgcgacga tgccgacggc cgcatcgacc ggcttcgtcg
      901 agcgcgatgg cgcgagcctc tactacgcga catacggcga cgcgaagaat ccaccggtcg
      961 tgctcctcca cggaggcatg ggcaacagcg atcactgggt gctccagatc cccgcgctca
     1021 ccgacaagct ctacgtcatc gcgatcgaca gccgcggcca aggccgcagc acgcgcacga
     1081 aggcgaagcc gtcctacgat ctaatggcgg acgacgtgct cgcggtgctc gacaagctcg
     1141 aggtcaagca cgccgcattc gtcggctgga gcgacggcgg tgagatcgcg ctcaagctcg
     1201 gcatccatca ccccgaccga gtcgacaagc tcttcgtgat gggcgcgaac tacgacgcga
     1261 atgggtccaa gccacgtagc gggaaaccgt cgcagacgtt cgccacgtac tcggcgcgct
     1321 gtcggaagga ctacgcgaag ctgtcgaaga ccccgaaaca gttcgaccag ctgacgcaat
     1381 ggctcttacc aatctggaag aacccgatgg gcttcacgaa ggatcagctg aagcagatca
     1441 aagcgccgac cgtgattgcc gacggagatc acgacgaaat catcaagctg tcgcagctcg
     1501 aagagatggc aaagctcatc ccgaacgcga agcttcgcgt gttcgcagac acgagtcatt
     1561 tcgtgctgtg gcaggacacc gagtcactca acgccgccct cgtggagttc ctcacagcta
     1621 actgagtcct atgtcgcgtt tgagcgcgat tcgctgaacg accatggtag acaatttcca
     1681 tgcgctcgct tcgccttgcc gccgttccgt tcgtcttgtt cgcgtgtggt gacaaccatg
     1741 atcagcagat gccggtcgat gcaaatcgtc ccgacacgat ggtcgacgcg ccacccgttc
     1801 caacgtgcga gttcaacgag gcgaacgacg ctggcaacga cgacctgttc ggcaccggca
     1861 cccccgaagc gacgaacatc gccttcgccg cgacgaagat gacggtctgc ggctctctca
     1921 acacgggcca cttcgacacg aactcactcc tgatcgactc cgactcgtac acggtgcatg
     1981 tcccgacgtc gcgcggcgcg agggtgaccc tgaccgggcc cggtgccgaa gccctcaaca
     2041 cggtcctgat cgagatctac gacaacgtct ccggtacgga tgcagtcggc acgttcctcg
     2101 gcaatcacgc cgcggcgggc gtcgatctcg tcgccggcga ctacacgatc accgtctcgg
     2161 cattccacac gacggcaccc gccgccgcga ttccgtatcg gctgacgctc tggcccgtcg
     2221 acctcgcgat ggcctgcccg cacgtgacga cgacggcgtc atacaccgag gcaaacgacc
     2281 tcgtgaccgc cgacggcaac gacgtctacg aggttcgcta cgggtcggcg accccgcacc
     2341 gcaagttcac ggacaacgcg ctcgacgcac ccgagaacac gacgaccgca ttcacgatcg
     2401 cgcctgcgat gaactatcgc gtgacgggcg tgaactcgtc gccgacggtg acgccggcgg
     2461 actgggccga ccgctaccaa gaccgcgaca cctacgcgat caagacaggc gcgaacacga
     2521 acgaagtctc gctgcgcctc aactggccgg gcacgacgat ggacatggac atgctcgtct
     2581 tcaaggagaa cgaccttacc gatttcgccg acgcgttctt gaacggaaac atggagccgg
     2641 agttcctgac gatcgcggtc cagccgaact cgacgtactg ggtgttcgtc gctgcggacg
     2701 atgcgtcgac ggggcagccc gtgaactatg atctgaccgt ctgcggcgct acgttcgcgc
     2761 cttgaagtgc cacagtccgg cgatctgatc ggcccatacc gcctcgtctc gcagctcgga
     2821 aagggcgcga tgggcgaggt gtggcgtgcg cgggatgagc gcctcgatcg atacgtcgcg
     2881 ctgaaggtcc tgcccgcgga gctagcaggc gatgcggagc ggcgcgctcg catgttgcgc
     2941 gaagcccgcg cggccgccgc catccgccat ccaaacgtcg tcacgctgtt cgacatcgtc
     3001 gagaacaagg acgacggcga ctcgctcgtc atggagctgg tcgaaggccg cacgctcgca
     3061 gacgtgctgc gcaaggaagc cgcgccgccg ctcgagcaag cgctccgctg gatcgaagga
     3121 gtagccgacg cactcgttgc cgctcacgcg aggcgaatcc tccaccgcga catcaaagca
     3181 gcaaacgtga tggtcacgac cgacggcggc atcaaggtcc tcgacttcgg cctcgcgaag
     3241 ctgcgcgacg acgcctcgcc ctccggatcc ggcaacatga tccccgcctc cgcgagcctc
     3301 gccgcgaaga tcgcgctcga cgagacgcag gcatcccagc aaggcgacag ctatcaaacg
     3361 cacgcgggct cgctgctcgg cacgccgctc tacatggctc ccgagcaaat agcaggtagc
     3421 gcccccgacg agcgaagcga ggtgttctcg gtcggcgtac tggcgtacga gatcctgcgc
     3481 ggcaagcctc cgtacaccgc gggcacgctc gaggatctat ttcgccagat cacgagcgag
     3541 gcgccaccac cgctcgaagg catcccggat ccgatcgcat ccgtggtgca ccaagcgctc
     3601 gaaaaggagc ccaccgcacg ctgggagtcg atgcgcgcgt ttcgcgacgc gatcgcggtc
     3661 gaacgaaagc gacgcttcgc acgcagcgca ccgcgctggc cagtcgtcgc agccgctctc
     3721 gtcctcctcg tcctcgccgt cggcggaacg gcactgtggt ggacgcggtc gcatcgagca
     3781 ccaccgccgc gacctggcga cgtgtacgtc actcgcgccc tcgacgagta caacctgttc
     3841 tacaacgaca aagcgctctc ctcgcttcgc gcggcccttc gcgagatacc cgaacacccg
     3901 cgcgcgaacg cgtacatgct gctgttcggc ggcgcgccgc ccgaagatct cgaagcagcg
     3961 ctcgcaggag caaaacgcgc actgccgaag accgcgctcc acagcaaaga ccgcgcgctc
     4021 gtcgaagcgg ccatcgccta ctcggaccgc ggcgcggccg aagcaaaggt ggccctactc
     4081 ggtgcaggtg cggcgcaaga tcgcgagctc gcattctggg ctgcagcgct cgactggcgc
     4141 tcgggcaact tccaagcggc gcatgcagga ttccaagcgc tgctcgcgca gcccgcgcgc
     4201 caattccgcg ggcgcatcta cgatctcgag tcgtcgatcc tgatctatct cgacgagccc
     4261 gccgaagctc ttcgcatcgg cacgctctat cgcgacgcat tccccggcga agccgatgca
     4321 gtcgcggtct acgccacaac gctcgccgtc aacggtcggt acgacgaagc gctcgtcgcg
     4381 gcagaagacg cgcgtcggtt gaacgagggc gaggacacgc tcgccggtct cgcaaaagtc
     4441 cacgcgctac gcggcgagcg cgccaaagcg aaggagctct accagcagtc gctcgagcgc
     4501 gccggagccg cccgtcgtcc gatccgtcgc gccgcgcttg cggtgctcca atgggccgac
     4561 ggcgagctcg acgccgcgaa ggagacggtg aagccgtgtc tctccggcgg agatgcgaca
     4621 gcgcgcgaac gcggtcaatg cctcttcgtc gctggcatca tcgagcccga acgcgccgaa
     4681 gccatcgcga caaagctcga cgagctggcc gccgaatcca cgccgacgcg accgtcgtat
     4741 ggattccctc gctcgctcgc aacgctgctg cgtgctcgtg cgaagttctt cggcggcgca
     4801 tgtctcgtcg atgttccgct cccttcagcg tcgatcgacg aggcctcgct cgacgtgcca
     4861 ctcgatttct acgccgcgta tcacgtgccg tttctggcga cgtggtcgat ttgcgagcgc
     4921 gccgcgctac gtgcagcgaa aggcgatcga gccggcgccg cagcgctact tcagccggtg
     4981 gcgaatcgcg cgccgaatcg cacgtggctg ctcaaggcgt tgcagcgcta ccagtagctg
     5041 ggatcgtgcc gggaagtggc ggcgccttcg cgcgattgat cgtttcgatg cgcgtcagga
     5101 tttcgccgcg gcgttcgttc cgcatgtccg gcgaagcgtc cagccgccgc aaacagcgct
     5161 tggcctcatc tagctgcccg agatcgcgtt cggcatcgca catcaccagc cacgcgtcga
     5221 tcttctcgtc gcgtctcgct tgccatgact cgacatccgt caggagctgg atcgcttcac
     5281 ggtatgcctt cttctcgacg agcatcttcg cgagacccgc acgcgcttgg aaatccggca
     5341 gacggtcgga gcagcgcctg ccggcgatat cggccgccag tagatcgccg tgctcggcga
     5401 gcatgaacat gagctcgcag ggctgggccg tcttcggacg cacgtcgagc acacgagcaa
     5461 gccagatccg ggcgacgtcc gctcgtttga gatcctcgag cgtcgtaacg acgagccgca
     5521 aatcgctgta ctcgagtggc aatgccgacg ctgcggtctc gccggggaac ttctcgagga
     5581 tctcggagag cagcggcttt ggttgctcgg tcatgcgcag ggccgctgcg tactcgatcg
     5641 cagcttgcga gttgaacttg tctgcgagga gcatgcgccc ggccatgcga tgcaattgtg
     5701 gatgcgtcgg atgtaacgcc atcgcgtggt tcagcaggcg aatcgcgcgg gggtccttct
     5761 tcgccgcgag gagctcggcg ccgacagcgt acgcgtagta atcgagcggg tggcgcgtcg
     5821 cggattcgcg cacgagtgcc atcgtcgcgt gcttcgacag gtcctgacga tcttcatcga
     5881 gcgtggtcgt cacgctggac atcagcaacg cggcaccggc cagcaatgcg actgcgtgca
     5941 ccacacgcaa gccacgagcg agccgaggcc tcaccgcctc gcgaagggga acgtacaaaa
     6001 cggtcgccgc cagcgcggtg acgggcgcgg caaggccaag gaactcgagc ccgaaatcga
     6061 cgttgctctg tgctgcaacg acggtcaacg cgccgagcac acctgcgctg agcgcgttat
     6121 ctcgccatcg tcgaatcgcc agccacacga accagaacgc gatcgcagcg agaatcagcg
     6181 cacccggaac gccgaagtcg acgaccgcct ggaggtactc gttctcgagg tatggatatg
     6241 tcgcaagtcc actggcctcg tgcacgcgag tgaacggcgc ctcgaatgca ccacggccga
     6301 cgccgagcca cacgttctcg tgaatcagct gtcccgccga gcgccaggcc acaaacttcg
     6361 atcgcgaaaa gtgaacctcg tcgaggctca actgcgagaa ctgtcgctcg acgttcgtgg
     6421 cgttcgacca aatcacgagc accgtcacac agccggcgag gattccgatc ggaagagcgc
     6481 tcgagagaaa ccgcgcacgc cgcttccgcg accggtcagc gcccgtcagg cgctgtgcga
     6541 cgagcgtgcc cacggtgatg aatgcacctg cgacgagcgc gatcgtgccg cctcgcgaaa
     6601 cagtcgcgac gacgatcgcg ccgcacagag gcacgagcaa gagccacatt gcgcgcaacc
     6661 agccaggctg cttgcgatgt gccgcgagcg cgattcccaa acacgcacct gccgccatga
     6721 ggcacgcgag cgtgttgtta ttgaggaccg gaccgaagac ctgcggatgt gcatcctgtg
     6781 gtttgtagat tccgtacacc gcgtcgagct cgaacagcgt gtgcacgcca tgcacgatcg
     6841 ccgtcactcc gcagagcacg gcgacgccgg cgacaagccg gtagcgacca cgctcgctcg
     6901 tcgcgatgcg tagcgcgagc atcgcgattc cgagcagtgc gaggaacacg atcaacgcac
     6961 ctagcgtcgc ggcggcgtca ctcgatgcgc ctgtccacgg agatgtgccg agcaagtccg
     7021 caccatccga gcgcagcgat gacgttgtcg gcgtgacccg atcgccgatg gcgcgaccca
     7081 gcggaagcag ctggagcgcg gtcaacgcgg cagcagctgc gatcagtgcg acgagtggag
     7141 agacgcgcga gaagccacgc tgtgagagcg ccgtcgcacc gacgcccatt gcgacgacgg
     7201 cggcgacgag cgcctgcgcc gtgcgaagca cgccgcccat ccacaagatc gaaagtgcaa
     7261 ctgcgacgag cagcgagacc gcgatcaccc aatcgcgagc gcgcattacc aagcacgttc
     7321 tggaacgaag atttcgtcac caggcatcat cggaaagtta ggccgcttgc cctcggcgat
     7381 catctcgacc ggaatcttgt acgtctcctt tttgccgtcg acggtgcgcg tgaccttcac
     7441 catgttcttg cgggcgagcg gcgtgaaccc accgctttgc gcgatggctt ccacgatcgt
     7501 catcccggga gtgaacttga ccgtgccgct cttgctcacc tgaccgagta cctggcactt
     7561 gagcgagttg atctcgacga cttgcagctg aacgatcggc tcgttcaggt agccgtcggc
     7621 gagccgcgaa cgaatctctt cctgcacctc gtgggtcgtc ttcccgttcg ccgaaaccgc
     7681 gccgatgtag cggacttcca tctgtccgga tgcatcgagg gtgtacgtct gcttttgttc
     7741 gttctgcccg tagaacaccg tgagctcgag cttgtcgccg ggaccgagcg cgagcttggc
     7801 cgggtcgaac ggagcaaccg tgggatagac cacaggcggg ttgtctccgc atgccacgaa
     7861 cgccaagaag gcgagaacaa gcaaacgcat cgcaggccgt tggatgtaat cgcgcaaccc
     7921 gtggttcatg ccaaaatccg gtgaacgtcg ccaggccacc tgacgtctta cggagctgag
     7981 attgactagt ttcgggcttt cacgtacctt tcagctccgt cgagcctact tcatgaaatc
     8041 tttcaggaaa tacacgatct tggccgcgct tttttgcggc acagtcgtgc aggctcagac
     8101 cgctccgccc ccaccgaagc cggtgtctgc gagcgatatg cgggtgcagt ccgcgacgat
     8161 caaaaagcag atcgacgacg acctccgcca cgtccttcac ctgcaagcac tcgctcgcaa
     8221 gagcaaggac gtcatcaagc tgacctgcat caacgacaag ctcgtgcaga tcaaggcgca
     8281 gctgaatctc tacgacaacg cgaacctgca gctcgagacg tcgatcaaca acggcgacgg
     8341 cacagcgagc acgtcgttca gcgacgtcga gaatacgggc gcggacgtga agaagctccg
     8401 cggcgaagcc gatggctgcg caggcgagct ggagctgtac aagcaagagt ccgcgacgaa
     8461 cgtggatcgt ccgccggatc tcgacgatcc gacggagact ggcggcttcg agccgcccga
     8521 gattcccgag ctcgagccgc ctggctacgc gacaccgttc aagtgatccg ctagtccgct
     8581 gcgagggtta cgtagttacg acgaccactg tgcgcgagcg ctcagtgagg aacctcggcg
     8641 caagtccgcg gccaactccg tcgacggccg cgatgacgat gtcatgcatg tccgttccgg
     8701 ttttgcggta ttcagcgtga acgcctgccc aggcgtctgc actaacaacg aacgagacga
     8761 tgccgctacg cctgttgaac aggaccgtcc aggtcgcgat cgacgcagtg attctgtcgc
     8821 tcgcgtattg gttggcgttc ctgtttcgcc tcgagttcgc gatctcggca acgtggttcc
     8881 gcgtcttgct cgtcacgtgg ccgtacgtcg taatcctcca gtacgcgtcg ctctcggcct
     8941 tcggcgtgcc tcgcctgtcg tggcgctaca tcagcattct cgacgtcacg cgcgtcttgt
     9001 cggcggtcac taccgcgaat gcggtgctgc tggtgattcg actcatggga ccgctcatcg
     9061 gtcctcacct cgagctcgtg gtcattccca tcggtgtgat cgcgatgaat ttcgtgctcg
     9121 ctttcttggg cctcgtcggc atccgcaccg ttcgccgcat gcaaggcgaa gcgagcgacc
     9181 gcaagcaaaa ggaagcgggg gcagagcgtc aacgcgtcct gctgatcggc gcggggcaag
     9241 ccggcgtgat ggtcgcgcgc gagatcatga atcgcccaga cctcggcctg catccggtcg
     9301 gtttcctcga cgacgatccg agcaagctgc ggacgagcat cggtggtttg ccggtgctcg
     9361 gtacaacggc ccagatccgc gaggtcgcgg agcgcaagca tgttcgccgc gcgctcatca
     9421 cgatcgcgaa tgcgccgggc aatcacatcc gacgcatcac cgagctatgt cgcgacgcag
     9481 agctcgagac gaagatcatt ccgggcatct acgagatcgt cggcgcgaaa gtgaacctct
     9541 cgcgaattcg cgaagtcgcg atcgaggact tgctaggtcg cgagccggtg cagctcgacg
     9601 aagatgtcgt cggcgcttcg attcgcagcc gcgtagtgct cgtcaccgga gccggcggaa
     9661 gcatcgggtc ggagctctgt cgtcaggtct cgcggttcgg tccgagccgt ctcgttcttg
     9721 tcgagcgcta cgagaacgca ctgttcgaga tccatcgcga gctgacgcag gcgttcccgc
     9781 atgtaccgat cgagccatgc atcgcagacg tctgcgacgc gacgcgcatg gagcaagtct
     9841 tcacggcaac gaagccggag ctcgtattcc acgccgcggc gcacaagcac gttccgatga
     9901 tggagtggaa tccgtgcgaa gccgtgaaga acaacatcgg cggcacgcgt gtcctcggcg
     9961 at
//
LOCUS       DATLBZ010000120         8082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9052594, whole genome shotgun sequence.
ACCESSION   DATLBZ010000120 DATLBZ010000000
VERSION     DATLBZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9052594"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VMZ53_06405"
     CDS             <1..928
                     /locus_tag="VMZ53_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS28119.1"
                     /translation="VYLAVGAQASASYFDSLAALRERLLFWGAGLAGVSVIAAAIATL
                     LITRNVRRLAAAAERIGTGDLRAPVAVTSRDELGVLAETMDRMRRQLAERDAKMQQML
                     AGIAHEVRNPLAGMTLFADILKDELPAGDEKRGHVEKIQRELGYLERVVNDFLEYARR
                     PRPELSSVDVPELFAEVAQMATTTEIELTVTPGGPSTVHADRAQLRRALLNLARNAVQ
                     AATAAEQRGKEVVKLSAKAKDDTVWLSVWNRGKEIPAETSGKLFEPFYTTREKGTGLG
                     LAFVRDIAVDHGGRVELESADGETTFSIVLPA"
     gene            961..2331
                     /locus_tag="VMZ53_06410"
     CDS             961..2331
                     /locus_tag="VMZ53_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS28120.1"
                     /translation="MATILIIDDNDTIREGLAITVKKLGHEAVTASSGTAGIAAFKQK
                     RADFVITDLKMEGGTGVDALKGISAMDPDVPIMIITGFGTVETAVEAMKLGAFDFITK
                     PIQSEVVRLKVERALEVCATKRNARKAIAQVDYHRGESDAKFAELVGGGDKMAVVRRT
                     VEKVAVSDTTVFIAGESGTGKELIARAIHRLSKRANGPFIKVNCGALTETLLESERFG
                     HEKGSFTGAIKQKLGRFELADGGTLFLDEVGDVPPSMQVKLLRALQEGEIDRVGGEAP
                     IKVDVRVLSATNKDLDAEVAAGRFRQDLFFRLHVLPVKLPPLRERREDIPLLVQHFIA
                     KLGPKTNARVRTVSDAALGRIMAYHWPGNVRELENAIEQALVFAEGEEIQPGALPQFL
                     QGGAEEDRLDVPREMSLPEILDDLERQLILKAYTKAGRVKTETARLLGIKTSALYYKL
                     EKYGIE"
     gene            2397..3869
                     /locus_tag="VMZ53_06415"
     CDS             2397..3869
                     /locus_tag="VMZ53_06415"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HUS28121.1"
                     /translation="MPLADTGIAGLDTILCGGLPRNRIYLIEGDPGVGKTTLALQFLL
                     AGVAKGERVLYVTLSESEQEIKQVAESHGWTLEGVSLFELSALQQQMSLEAQNTVFHP
                     SDVELTETTRAILNYVEKVNPQRVVFDSLSELRLLAQNPLRYRREVLNLKQYFAGKLS
                     TVLLLDDRTSEPGDMQLQSLAHGVISMQQHAIDYGGDRRRIRVAKLRGRKFITGYHDY
                     AVRTGGLEVFPRLVAAAQHEAEYKRDEVSSGIPALDKMLDGGLTRGTSTLILGPAGTG
                     KTAIASAYACAAAMRGERVVMFEFDELRRTTIERADSLGMKFSEAVAAGNLVVQQIDP
                     AEMSPGELVHRARELIENQGFKLIVFDSLNGYLHSMPAEKYLYIQLHELLTYFGQLGA
                     TTLMVMAQSGIVGTTVSPADISYIADSVMMLRYFEAAGRVRKAISVIKKRTGKHEDTI
                     RELTLGGKDGITLGEPLRDFRGILTSLPEFFGDRQKLEES"
     gene            3866..5395
                     /locus_tag="VMZ53_06420"
     CDS             3866..5395
                     /locus_tag="VMZ53_06420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HUS28122.1"
                     /translation="MSPTSNLPVLVFAPRGRDGELIERALTANGFATVMCTTPAELLE
                     AMRDDAGCALITQEALDASVRDELAAWLSTQPPWSDFPLLVLLSAASPDTLESLSRRL
                     GNITFLERPLAPATLLTATRSALRGRMRQLEARSAIQQRDQFLAMLGHELRNPLAAIV
                     LAARLVREGSDRSQLGHRVEVIERQSNILARLVDDLLDVARVTSGKVRLRSEAVDVDA
                     VIRSCIEAHEERAKRRNMQIVFTSASNVTIEGDTVRIEQVVSNLLANAVKYSPAGRTV
                     RISSKRLDDACEIRVRDEGIGIAPEMQSHVFDLFTQIDSSADRSEGGMGIGLTLVDRL
                     VRLHGGTIDLVSDGLGTGSEFIVRLPIGVPPVDPSVLKLEPAIAAQKIRVVLVEDNAD
                     LRELSAEMLEALGCSVEVAVDGPEGVERICSSHPDLALVDIGLPLLDGFGVARQVRER
                     IGREQMLVAVTGYGREQDREQAQAAGFDLHVTKPLAADTLKGLIERARARRTRSAASG
                     S"
     gene            5478..5888
                     /locus_tag="VMZ53_06425"
     CDS             5478..5888
                     /locus_tag="VMZ53_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HUS28123.1"
                     /translation="MIDLPFDRARLETWTYTALRFVAGFMFFWHGAQKILGVLSTKAP
                     PEVFSQMWIGGVIELVGGALIALGMLTRPAAFICAGQMAVAYIQFHWKGALDASFLPI
                     VNKGESAVLYCFIFLVFAVRGAGPISIDRQLGRA"
     gene            complement(5913..6956)
                     /locus_tag="VMZ53_06430"
     CDS             complement(5913..6956)
                     /locus_tag="VMZ53_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HUS28124.1"
                     /translation="MEGTLSLFDWRPTASATPASTAPEPHVADLTPTAVPAAGPRIIV
                     FDCETTGIDFTRDQVIELCVQHGLGDQATSRTWRIKPSVAIHPAAQAVHGITMADLAS
                     CASFAEYADEIAMVFAGADVIVGYNLSFDIDMIQAEYSRIGRPLLDLSNKKIVDAFRL
                     WQQCEPRSLQHAHRRFVGDEFAAAHSASADVAATGRVLVGMLDHFKLAGQDWTQIAHV
                     CDPGRASWVGPSRHLRWENEVVVLGFGKHAGAPLHELASGPDRSFLQWVITKDFPPHV
                     MEICKAALEHDCDALHAWVRQRYGQPAPTAQPPFAAPAPPSAAPPSPVASEPNAAKPR
                     AKSKKSRTSTEAP"
     gene            7007..7621
                     /locus_tag="VMZ53_06435"
     CDS             7007..7621
                     /locus_tag="VMZ53_06435"
                     /inference="COORDINATES: protein motif:HMM:NF023669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28125.1"
                     /translation="MRWPVIVLTLAACKVKDPPPVTEEWKDGFERGDIGGDYYATGKG
                     FDIVSGQLSARGAHNHPLWLRKKLPHDVRIELDCWSNEERGDVKVEVFGDGKSFDPDA
                     GRYTATGYEVIFGGWFNSKSIIARLDEHGKDVVENTQLKVQPTRHYHWTIERIGKKLT
                     WTIDQQPFLVLDDPNPLEGPGHEYFGFNNWESDTWFDNLVITPL"
     gene            7643..>8082
                     /locus_tag="VMZ53_06440"
     CDS             7643..>8082
                     /locus_tag="VMZ53_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28126.1"
                     /translation="MEQFGKYTLIRKIGTGGMAEVYLARTVVAQGLNKTLVIKKIHTA
                     YARSRQFVTMFVDEAKIALGLNHPNIIQVFDFGAVGDTYFLAMEYVEGMDLLRLLQEA
                     AKARQRLPYGISAYTVQQLAKGLDYAHRKADEFGQPLGIVHRDIS"
BASE COUNT         1468 a         2600 c         2662 g         1352 t
ORIGIN      
        1 ggtctatctc gccgttggtg cgcaggcatc ggcgtcgtac ttcgacagcc tcgcggcatt
       61 gcgcgagcgc ttgctgttct ggggcgctgg gctcgccggc gtgagcgtga tcgcggcagc
      121 gatcgcgacg ctgctgatca cgcgcaacgt gcgccggctc gctgcggcag cggagcggat
      181 cggcaccggt gaccttcgcg cgcccgtcgc ggtgacgtcg cgcgacgagc tcggcgtgct
      241 tgccgagacg atggatcgga tgcgccgtca gctcgccgaa cgcgacgcga agatgcagca
      301 gatgctcgcc ggcatcgcgc acgaggttcg caacccgctc gccggcatga cgctgttcgc
      361 cgatattctg aaggacgagc tgcccgccgg cgacgagaag cgcggccacg tcgagaagat
      421 ccagcgcgag ctcggctacc tcgagcgcgt cgtcaacgac ttcctcgaat acgcgcggcg
      481 acctcgcccc gagctgtcga gcgtcgacgt gcccgagctg ttcgccgagg tggcgcagat
      541 ggccacgacc acggagatcg agctgaccgt tacgcctggc ggtccgtcaa ccgtgcacgc
      601 cgaccgcgcg cagctgcgtc gcgcattgct gaacctcgca cgcaacgcgg tgcaagcagc
      661 gaccgcggcc gagcagcgcg gcaaagaggt cgtgaagctc tcggcgaaag cgaaagacga
      721 cacggtgtgg ctctcggtgt ggaaccgcgg caaggagatt cctgccgaga cgagcggcaa
      781 gttgttcgag ccgttctaca cgacgcgcga aaagggcacg ggcctcgggc tggcgttcgt
      841 acgcgacatc gcggtcgacc atggtggccg cgtcgagctc gagagcgccg acggagaaac
      901 gacgttttcg atcgtgcttc ccgcgtaggc cggcttcgcg tgtacgatgg gcccgcgcgc
      961 atggcgacca tcctcatcat cgacgacaac gacacgattc gcgaaggtct cgcgatcacc
     1021 gtcaagaagc tcggtcacga ggcagtcacc gcatcgagcg gcacggctgg catcgccgcg
     1081 ttcaagcaga agcgcgccga cttcgtgatc accgatctca agatggaggg cggcaccggc
     1141 gtcgatgcgc tcaagggcat cagcgcgatg gatcccgatg tgccgatcat gatcatcacg
     1201 ggcttcggca ccgtcgagac cgcggtcgaa gcgatgaagc tcggcgcgtt cgacttcatc
     1261 acgaagccga tccagagcga ggtcgtgcgg ctcaaggtcg agcgagcgct cgaggtctgc
     1321 gcgacgaaac gcaacgcacg caaggcgatc gcgcaggtcg actaccaccg cggcgagagc
     1381 gacgcaaagt tcgccgagct cgtcggcggt ggcgacaaga tggccgtcgt ccgtcgcacc
     1441 gtcgagaaag tcgcggtcag cgatacgacc gtgttcatcg ccggcgagtc gggcaccggc
     1501 aaggagctca tcgcgcgcgc gatccatcgc ctgtcgaagc gcgcgaacgg cccgttcatc
     1561 aaagtgaact gcggcgcgct gacggagaca cttctcgaga gcgagcgatt cggtcacgag
     1621 aagggctcgt tcaccggcgc gatcaagcag aagctcggcc ggttcgagct cgccgacggc
     1681 ggcacgctgt tcctcgacga ggtcggcgac gtcccaccgt cgatgcaggt aaagctcctg
     1741 cgcgcgctgc aagagggcga gatcgatcgc gtcggtggcg aggcgccgat caaagtcgac
     1801 gtgcgcgtgc tctcggcgac gaacaaggat ctcgatgccg aggtcgccgc gggccggttc
     1861 cggcaagacc tgttcttccg cctccacgtg ttgcccgtga agctgccgcc gctgcgcgag
     1921 cgtcgcgaag acattcccct gctcgtccag cacttcatcg cgaagctcgg gccgaagacg
     1981 aacgcgcgcg tgcgcaccgt cagcgatgcg gcactcggtc gcatcatggc ctatcactgg
     2041 ccgggcaacg tgcgagagct agaaaacgcc atcgagcagg ccctggtatt cgccgagggg
     2101 gaagagatcc agcccggtgc actgccccag tttctccagg gcggcgccga ggaagaccgc
     2161 ctcgacgtac cgcgcgaaat gtcactcccc gagatcctcg acgacctcga gcgccagctc
     2221 attctgaagg cctacacgaa agccggacgg gtaaagaccg agacggcacg actgctggga
     2281 atcaagacga gcgcccttta ctacaagctc gagaagtacg ggatcgagta gagatcacgg
     2341 tcatctaacg cagcgccaca gaagacaggc gctcggtgtc gaagtaattt cggctaatgc
     2401 cgctcgcaga cacaggcatt gccggactgg acacgattct gtgcgggggc ctgccgcgaa
     2461 accggatcta cctcatcgaa ggtgacccgg gcgttggcaa gaccacgctc gcattgcagt
     2521 tcttgctagc gggtgtcgcg aagggcgaac gcgtcctcta cgtcacgctc tcggagagcg
     2581 agcaggagat caagcaggtc gcggagtcgc acgggtggac gctcgagggc gtgtctctgt
     2641 tcgagctgtc ggcgctgcag cagcagatga gcctcgaggc gcagaacacg gtcttccatc
     2701 cgtcggatgt cgagctcacc gagacgacaa gagcgatcct caactacgtc gagaaggtga
     2761 acccacagcg cgtggtgttc gactcgctgt ccgagcttcg actgctggcg caaaatcccc
     2821 tgcgctatcg gcgcgaagtc ttgaacctca agcagtactt cgccggcaag ctgtcgacgg
     2881 tattactcct cgacgaccgc acgtccgagc ccggcgatat gcagctgcag agcctcgcgc
     2941 acggcgtgat ctcgatgcag cagcatgcaa tcgactacgg cggcgaccgg cgccgcatcc
     3001 gcgttgcgaa gctgcgcggc aggaagttta tcaccggcta tcacgattac gcggttcgca
     3061 cgggcgggct cgaggtcttc ccgcgcctcg tcgccgcggc tcaacacgaa gccgagtaca
     3121 aacgcgacga agtgtcgagc gggatcccag cgctcgacaa gatgctcgac ggcgggctca
     3181 cacggggaac tagcacgctg atcctcgggc ccgcgggaac gggcaagacc gcgatcgcga
     3241 gcgcgtacgc gtgtgccgct gcgatgcgcg gggaacgcgt cgtgatgttc gagttcgacg
     3301 agctccgtcg gaccacgatc gagcgtgcag attcgctcgg catgaagttc agcgaggcgg
     3361 tggccgcggg caacctcgtc gtgcagcaga tcgaccctgc ggagatgagc cctggggagc
     3421 tcgtgcatcg cgctcgcgag ctcatcgaga accagggctt caagttgatc gtcttcgaca
     3481 gtctcaacgg ctacctgcac tcgatgcccg ccgagaagta cctctacatc cagctccacg
     3541 agctgctcac gtacttcggc cagctcggag caacgacgct gatggtgatg gcgcagtcag
     3601 gcatcgtcgg cacgaccgtc tcgccggcgg atatcagcta catcgccgac agcgtgatga
     3661 tgctccgcta cttcgaagcc gctggacgcg tgcggaaggc gatctcggtc atcaagaagc
     3721 ggacagggaa gcacgaagac acgatccgcg agctcacgct cggcggcaaa gacggaatca
     3781 ccttgggcga gcccttgcgc gactttcgcg gcatcctcac atccctgccg gaattttttg
     3841 gcgatcggca gaagctcgaa gagtcgtgag tcccaccagc aatctccccg tgctcgtgtt
     3901 tgcgcctcgc ggccgcgatg gcgagctcat cgagcgcgcg ctgacggcga acgggttcgc
     3961 gacggtcatg tgcacgacgc cggccgagct cctcgaagcg atgcgagacg acgcgggctg
     4021 cgcgctgatc acgcaagaag ctctcgacgc gtcggtccgc gacgagctcg cggcctggct
     4081 ctcgacgcaa ccaccgtggt ccgatttccc gctgctcgtt ctgctcagcg ccgcatcgcc
     4141 agacacgctc gagtcgctgt cgcggcggct cggcaacatc acgttcctcg agcgtccgct
     4201 cgcaccggcg accctgctca ctgcgacccg ttctgccctg cgcggccgca tgcgccagct
     4261 cgaagcacgc tcggcaatcc agcagcgcga tcaatttctc gcgatgctcg gccacgagct
     4321 ccgcaatccg ctcgccgcca tcgtgctcgc agcgcgcctc gtgcgcgagg gcagcgatcg
     4381 ctcgcagctc ggccatcgtg tcgaagtgat cgagcggcag tcgaacatcc tcgcgcgcct
     4441 tgtcgacgac ctcctcgatg tcgcccgcgt gacgtcgggc aaggttcggc tccgcagcga
     4501 ggccgtcgac gtcgatgcgg tgattcgcag ctgcatcgag gcgcacgagg agcgcgcgaa
     4561 gcgtcgcaat atgcagatcg tcttcacgag cgcctcgaac gtgacgatcg aaggcgacac
     4621 ggttcgcatc gagcaggtgg tgtcgaatct gctggcaaac gccgtcaagt acagccccgc
     4681 cggccgtacg gtccgcatct cgtcgaagcg cctcgacgac gcgtgcgaga tccgtgttcg
     4741 cgatgaaggc atcggcatcg cgcccgagat gcaatcgcac gtgttcgatc tgttcacgca
     4801 gatcgacagc agcgccgatc ggtcagaagg cggcatgggc atcggtctca cgctcgtcga
     4861 ccggctcgtt cgcctccacg gcggcacgat cgacctcgtc agcgacgggc tcggcaccgg
     4921 cagcgaattc attgtccggc tcccgatcgg tgtcccgccg gtcgatccct ccgtgctgaa
     4981 actcgagccc gcgatcgcgg cccagaaaat tcgtgtcgtt ctcgtcgagg acaacgcaga
     5041 cctgcgcgag ctctcggccg agatgctcga agcgctcggt tgttcagtgg aggtagcggt
     5101 cgacggaccg gaaggtgtcg agcgcatctg ctcgagccat cccgatctcg ctcttgtcga
     5161 tatcggcttg ccgctcctcg acggattcgg cgtagcgcga caggttcgcg agcgcatcgg
     5221 tcgcgaacag atgctcgtgg cagtgaccgg ttacggacgc gagcaggatc gagagcaggc
     5281 gcaggccgcg ggcttcgacc tgcacgtcac gaagccgctc gcggcggaca cgctcaaggg
     5341 cttgatcgag cgggcgcgtg cccgtcgcac tcgctctgct gcatccggct cgtaacgcac
     5401 accggtgtac ctaacgcgcg tgaatgtgcc cgcgagcgca acgtttcttc acgaagctcg
     5461 cgtgctaggt ctacggcatg atcgatctgc ctttcgatcg cgcccggctc gagacctgga
     5521 cgtacaccgc actgcggttt gtcgccgggt tcatgttctt ctggcacgga gcccaaaaga
     5581 ttctcggcgt cctctcgacg aaagcgccgc cggaggtgtt ctcgcagatg tggatcggcg
     5641 gcgtgatcga gctagtcggt ggggcgctga tcgcgctcgg catgctgacg cggcccgcgg
     5701 cgtttatctg cgcggggcag atggcggtcg cgtatatcca gttccactgg aagggcgcgc
     5761 tcgacgcgtc gttcctgccg atcgtgaaca agggcgagtc cgcagtgctc tactgcttca
     5821 tcttcctcgt gttcgccgtc cgcggcgctg ggccgatctc gatcgatcgg cagctcggtc
     5881 gcgcataagc gagcgacctt cgcaacgcgc ggttacggcg cttccgtcga cgtgcggctc
     5941 ttcttcgact tcgcgcgcgg cttcgcagca tttggctctg atgcgacggg cgacggcggc
     6001 gccgcggacg gtggcgcggg cgcggcgaac ggtggctgcg ccgtcggcgc tggctggccg
     6061 tagcgctggc gtacccacgc gtggagagcg tcgcaatcgt gctcgagcgc ggccttgcag
     6121 atctccatga catgcggcgg gaaatctttc gtgatcaccc actgcaagaa gctgcgatcg
     6181 ggccccgagg cgagctcgtg cagcggagcg ccggcatgct tgccgaagcc aagcacgacg
     6241 acttcatttt cccagcgcag gtggcgtgac ggaccgaccc agctcgcgcg acctggatcg
     6301 cagacgtgcg cgatttgcgt ccagtcttgg ccagcgagct tgaagtgatc gagcatgccg
     6361 acgagcacgc ggcccgtcgc ggcgacatcg gcgctcgcgg agtgcgcggc ggcgaactcg
     6421 tcaccgacga agcggcgatg cgcgtgctgc agcgagcgcg gctcgcactg ctgccagagg
     6481 cggaacgcat cgacgatctt cttgttcgac aggtcgagta gcgggcggcc gatgcggctg
     6541 tactccgctt ggatcatgtc gatatcgaac gacaggttgt agccaacgat gacgtcggcg
     6601 ccggcgaaga ccatcgcgat ctcgtcggcg tactccgcga acgatgcgca cgacgcgagg
     6661 tcggccatcg tgatgccgtg cacggcttgc gcggcgggat ggatcgctac gcttggcttg
     6721 atccgccacg ttcggctcgt cgcctgatcg cccaggccgt gctggacgca gagctcgatg
     6781 acttggtcgc gcgtgaaatc gatgcccgtg gtctcgcaat cgaacacgat gatgcgcggg
     6841 ccggccgcgg gcaccgccgt gggcgtgaga tccgcgacgt gcggttcggg cgccgtggac
     6901 gcaggtgtcg ctgacgccgt cgggcgccaa tcaaacagcg acagagtgcc ttccacacga
     6961 gaagcctacg gtgccggtct gacatcattt ggggtagcct ccccgcatgc gttggcctgt
     7021 catcgtcctc acgttggctg catgcaaagt gaaagatcca ccgccggtta cggaggagtg
     7081 gaaggacggc ttcgagcgcg gggacatcgg cggcgactac tacgcgacag gcaagggttt
     7141 cgacatcgtc agcggtcagc tgtctgcgcg cggcgcgcac aatcacccgc tctggctgcg
     7201 caagaagctc ccgcacgacg tgcgcatcga gctcgattgt tggtcgaacg aggagcgcgg
     7261 cgacgtcaag gtcgaggtct tcggcgacgg caagtcgttc gatccggatg cgggtcgcta
     7321 cacggcaacc ggctacgagg tgatcttcgg cggttggttc aactcgaagt cgatcatcgc
     7381 gaggctcgac gagcacggca aagatgtggt cgagaacacg cagctgaagg tccagccgac
     7441 acgccactac cactggacca tcgagcgcat cggcaagaag ctgacctgga cgatcgacca
     7501 gcaaccgttt ctcgttctcg atgatccgaa cccgctcgaa ggacccgggc acgagtattt
     7561 cggcttcaac aactgggagt cggatacctg gttcgacaac ctcgtgatca cgccccttta
     7621 agctaaaatc gtccccgaac gggtggagca gttcggtaaa tacacgctga tccggaagat
     7681 cggcaccggc ggtatggccg aggtctatct cgcgcgcacc gtggtcgcgc agggactgaa
     7741 caagaccctc gtcatcaaga agatccacac cgcgtacgcg cggtcgcggc agttcgtcac
     7801 gatgttcgtc gacgaagcga agatcgcgct cggcctgaac cacccgaaca tcatccaggt
     7861 cttcgacttc ggtgccgtcg gcgacacgta cttcctcgcg atggagtacg tcgagggcat
     7921 ggatctcctg cggcttctgc aggaggcggc gaaggcgcgg cagcggctcc cgtacggcat
     7981 cagcgcgtac accgtgcagc agctcgcgaa gggcctcgac tacgcgcacc gcaaggccga
     8041 cgagttcggt cagccgctcg gcatcgtgca tcgcgatatc tc
//
LOCUS       DATLBZ010000121         5163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1611397, whole genome shotgun sequence.
ACCESSION   DATLBZ010000121 DATLBZ010000000
VERSION     DATLBZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611397"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VMZ53_06445"
     CDS             complement(<1..428)
                     /locus_tag="VMZ53_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28127.1"
                     /translation="MRGMSLALLVVTASIGNAFADEPATTETNETSGTVGGAPGDTPA
                     TGESRPADKGTLGVGLVIGEPTGISAKLYLEDDQAIQGAIGPAFIGGGIALHGEYVLH
                     PWILQDRDSFVLPVYLGPGVRFVDYYGGRSTGTSSSHFAVG"
     gene            566..1099
                     /locus_tag="VMZ53_06450"
     CDS             566..1099
                     /locus_tag="VMZ53_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28128.1"
                     /translation="MLVAVLARVLAQIILVLCFAAGMAASCIIFVLYGPVPVRRKVTI
                     VPRLSPPDVFAIRAVKVVSLPAPVEAVQQIEPGIAFRTPPAPPQAPARAKKSEPRPGT
                     PDVAPLVLRRPRGDSPAPLPRSRSARGTERRPRPSELDITSVRSLESNRSFETSDNTL
                     ETPLVELGDDQFLEDGR"
     gene            complement(1096..2679)
                     /locus_tag="VMZ53_06455"
     CDS             complement(1096..2679)
                     /locus_tag="VMZ53_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HUS28129.1"
                     /translation="MLVRSMELNQAEQRFELLADLGAMIAGEVELDDMLATFADRVAK
                     ALGADRATLWLIDGETGDIRSRVATLPELSGPLVVPQGHGVVGHVAATGELVNVRDAA
                     TDPRWGGGELAQQIGYRTTSILTAAIVRRGQIRGVLQVLNKRDGAFTSRDEDFARVLA
                     EQIGRALDYTTLRGDDSSRGLSVRGRFNHVIGRSPAMAAVYEMIVRAAQTDATVLLHG
                     ETGTGKGLLARAIHVNSERKNAPFVHVDCTTLPAALVESELFGHERGAYTGADSRVIG
                     KVEAANGGTLFLDEIGELPLPLQGKLLRFVQERQFERVGGRETLTADVRIVAATNRDL
                     DAMSASNQFRSDLYFRLRVVEIEIPPLRTRGEADINDLAEHFVQQFSRRYRKGPLRLS
                     DSARPAIVAYSWPGNVRELENSIERAVVLAAHPTLSVKDLGLDRQRLTNAGDSQVTIP
                     VIHVPPVAPAASTAPVDGVLLPNDLPLEEVERRYAKAMFDRENGNQSAAARVLGISRN
                     KLARLIKGEKPDDKDDEEV"
     gene            complement(2697..3533)
                     /locus_tag="VMZ53_06460"
     CDS             complement(2697..3533)
                     /locus_tag="VMZ53_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS28130.1"
                     /translation="MSNDLTVRFLGVRGSIATPGMAAGGNTACLEVVAGDTRIILDAG
                     TGLRTLGNERMQQGIRHSTILLSHLHWDHVAGLQFFTPVYVPGHRVEIASGPNGVMSH
                     EEAIRSLFKAPFFPVAFDDLGGNVITRELRPNDRFEIGGITVTMARLNHPDPVYGYRL
                     EHAGQSIVYATDTEHFSCVDPTLKKLAAGADILIYDAQYTPEEYPAKVGWGHSTWEAG
                     ADLARAAGVPQLVLFHHDPGRTDAQLAALEAAAAAALPGTIAAREGMVLSARDAEKAA
                     AA"
     gene            complement(3648..5111)
                     /locus_tag="VMZ53_06465"
     CDS             complement(3648..5111)
                     /locus_tag="VMZ53_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HUS28131.1"
                     /translation="MSLAHKAARGALWTIISSMGGRAVGVIGTLVITRFLAPEVIGEV
                     SDAAIITMTANWITIWGFGQYTVVKGRGEDRKEVIWHATFFYLVLGIISLGAVAAFGG
                     FFAPIFNAEKAAQYIPGMAIAVFIRRLGAMPERLLTQQMNFRPSGMALALGEVAFTAT
                     ALPMAAYGWGGWSMIVANIVKSIVATWILISAAGVRSWFSPTQLSWKRTKDMLGFGVP
                     LGIQGVAHMASRYWDNLAISYYFGTGYLGAYNMAYNLADIPAVQVGEQIALVLLPSIA
                     ELPKDRRAKALERSTALLSLIIFPLAVGLGLIAYPLIAWILPKNSWQDVAPLLTVLAC
                     LSVFRPITWVLSAYMEAEQKTGQLMFLEIAKVAFIVGGIGVMANWGIRASAGAVGLAF
                     GLTALAGVAMVVRHGPSPTRLAAGFIQPLAACAFMAAAVWLVHAGLRGIGVEHPSIYV
                     IVEIVVGALAYVGAALVLCRETSKDLLQLAVKALKRS"
BASE COUNT          908 a         1720 c         1685 g          850 t
ORIGIN      
        1 ccgaccgcga agtgcgagct gctagtgccg gtgctgcgac cgccgtagta atcgacgaac
       61 cgcacgccgg gcccgaggta cacgggcaac acgaacgaat cgcgatcctg caggatccac
      121 ggatggagca cgtactcgcc gtgcagcgcg atgccgccac cgatgaatgc gggcccgatg
      181 gcgccctgga tcgcctgatc gtcttcgaga tagagcttcg cgctgatgcc cgttggctcg
      241 ccgatgacga ggccaacgcc gagcgtcccc ttgtcggcgg gacgtgattc gccggtcgcg
      301 ggagtgtcgc cgggcgcgcc gcctaccgtt ccgctcgtct cattggtctc ggtcgttgcc
      361 ggctcgtcgg caaaggcgtt gccgatagag gcggtcacga cgagcagggc cagagacatc
      421 ccacgcatgg cgcgacgcta cctgccgggg caggtgagcg caaattcgct gggccgcgga
      481 gtgcggggtc gatccccacc catggggacc gctctcctcg ggggccatca cgatcgatca
      541 atgcgttgcg acgttcgagc gcggcgtgtt ggttgcagtc ctcgcgcgcg tgctcgcgca
      601 aatcatcctg gtcctgtgtt tcgcagcggg tatggcggcg tcgtgcatca tctttgttct
      661 gtacggaccg gtaccggttc gccgcaaggt gaccatcgtt ccgcgtctct cgccgcccga
      721 cgtgttcgcg attcgcgccg tcaaagtcgt atcgctgccg gcgcccgtcg aggccgttca
      781 gcagatcgag cccggcattg cattccgcac cccgccggca ccgccgcagg ctccggcgcg
      841 cgccaagaaa agcgagccgc gtccgggcac gcccgatgtc gcgccgctcg tgctgcgtcg
      901 cccccgtggt gatagcccgg cgccgcttcc gcgctctcgt tcggcgcgtg gcaccgagcg
      961 tcgcccgcgt ccgtcggagc tcgacatcac gtcggtgcgc tcgctcgaga gcaaccgatc
     1021 gttcgagacg tcggacaaca cgctcgagac tccgctcgtc gagctcggcg acgatcagtt
     1081 cctcgaagac ggccgctaga cttcttcgtc gtctttgtcg tcgggcttct cgcccttgat
     1141 gagacgcgcg agcttgttgc ggctgatgcc gagcacgcga gcagcggccg actggttgcc
     1201 gttctcgcga tcgaacatcg cctttgcgta gcgacgctcg acctcttcga gcggcaggtc
     1261 gttcggcagg agcacgccat caacaggagc cgtcgacgcg gcaggcgcga ccggcggcac
     1321 atggatcacg gggatcgtga cctgcgagtc accggcgttc gtcaggcgct gccgatcgag
     1381 gccgaggtct ttgacggaga gcgtcgggtg cgccgcgagg acgactgcac gctcgatcga
     1441 gttctcgagc tcgcgcacgt tgccgggcca gctgtacgcg acgatcgcag gacgcgcgga
     1501 gtcagagagg cgcagtgggc ccttgcgata gcgacgcgag aactgttgaa cgaagtgctc
     1561 ggcgagatcg ttgatgtcgg cttcgccacg cgtgcgcagc ggcgggattt cgatctcgac
     1621 gacgcgcagg cggaagtaca ggtcgctgcg aaattgattg ctcgccgaca tcgcgtcgag
     1681 atcgcgattg gtcgcggcga cgatgcggac gtccgccgtc agcgtctcgc ggccaccgac
     1741 gcgctcgaac tggcgctcct gcacgaaccg cagcagcttg ccttgtagcg gaagtggcag
     1801 ctcgccgatc tcgtcgagga acagcgtgcc gccgttcgcc gcttcgacct tgccgatcac
     1861 gcgcgagtca gcgccggtgt atgcgccacg ctcgtggccg aacagctcgc tctcgacgag
     1921 tgcggcgggg agcgtcgtgc agtcgacgtg gacgaacggc gcgttcttgc gctcgctgtt
     1981 gacgtggatc gcgcgcgcga gcaggccctt gcccgtgccg gtctcgccgt gaagcaggac
     2041 ggtcgcgtcg gtttgcgcgg cgcgcacgat catctcgtag acggcggcca tcgcgggcga
     2101 gcgaccgatg acgtggttga agcgaccgcg tacggagagg ccgcggctcg agtcgtcgcc
     2161 acgcagcgtc gtgtagtcga gcgcgcgacc gatttgctcg gcgagcacgc gcgcgaaatc
     2221 ttcatcgcgt gacgtgaacg cgccgtcgcg cttgttgagc acctgcagca cgccgcggat
     2281 ctggccgcgt cgaacgatcg ccgccgtgag gatcgacgtc gtgcggtaac cgatctgctg
     2341 cgcgagctcg ccgccgcccc accgcggatc ggtcgcggca tcgcgaacgt tgacgagctc
     2401 gcccgtcgct gcgacgtgac cgacgacgcc gtggccttgc ggcacgacga gcgggcccga
     2461 gagctcggga agcgtcgcga cgcgcgagcg gatatcgccg gtttcgccgt cgatgagcca
     2521 caacgtcgcg cggtctgcac cgagcgcctt cgcgacgcga tcggcgaacg tcgcgagcat
     2581 gtcgtcgagc tcgacctcgc ctgcgatcat cgcgccgaga tccgcgagga gctcgaagcg
     2641 ttgctcggct tggttcagct ccatgctccg gaccagcatc tcacgaacgg ccggcgtcat
     2701 gctgcagcgg ccttctccgc atcgcgcgcc gatagcacca tgccctcacg cgcagcaatc
     2761 gtacccggca gtgcggctgc cgctgcggcc tcgagcgcag caagctgtgc gtcggtgcgt
     2821 cccggatcgt gatggaacag cacgagctgc ggaacacctg cggcgcgcgc gaggtctgcg
     2881 cccgcttccc acgtcgagtg gccccagcca accttcgccg gatactcctc gggcgtgtac
     2941 tgcgcgtcgt agatcaagat gtctgcgccg gccgcaagct tcttgagcgt ggggtccacg
     3001 cacgagaagt gctcggtgtc ggtcgcgtac acgatcgact ggcccgcatg ctcgaggcga
     3061 tagccgtaca cgggatccgg atgattgaga cgcgccatcg tcacggtgat gccgccgatc
     3121 tcgaagcgat cgttcggacg cagctcgcgc gtgatcacgt tgccgccgag atcatcgaac
     3181 gcgactggaa agaacggcgc cttgaagagc gaacggatcg cctcttcgtg gctcatcacg
     3241 ccgttcggac cactcgcgat ttcgacgcga tgccccggca cgtacaccgg cgtgaagaac
     3301 tgcaggcctg caacgtgatc ccagtgcagg tgcgaaagga gaatcgtcga atggcgaatg
     3361 ccttgctgca tgcgctcgtt accgagcgtg cgaaggcccg tacccgcatc gaggatgatg
     3421 cgcgtatcgc cagcgacgac ctcgagacac gcggtgtttc cacccgcggc catacccgga
     3481 gtcgcgatcg atccgcgaac accaagaaag cgaacggtca ggtcgttaga catatgcact
     3541 acctcctcga cccggagata gagcaccttc ggtgccacgt ctaactacgc gacatcccga
     3601 agaatacctg cttcattcgg tggcacctgc ttcaaaatgg agcaggtcta gctccgcttc
     3661 agagccttca cagcgagctg caacagatcc ttggacgtct ctcggcacag caccaacgca
     3721 gcgccgacat acgcgagagc gccgaccacg atctcgacga tcacgtagat cgacgggtgc
     3781 tctacgccga tgccgcgcaa tcccgcgtgc acgagccaca cggccgccgc catgaacgcg
     3841 cacgccgcga gtggctgaat gaaaccagca gcgagccgcg tcggcgaagg accatggcgc
     3901 accaccatcg cgacgcccgc gagtgccgtc agaccaaacg ccaagccgac tgcacccgca
     3961 ctcgcgcgaa tgccccagtt cgccatcacg ccgatgccgc cgacgatgaa cgcgaccttc
     4021 gcgatctcga ggaacatcag ctggccggtc ttctgctcgg cttccatgta cgcgctcagc
     4081 acccacgtga tgggacggaa tacggagagg cacgcgagca ccgtgagcag cggcgcgaca
     4141 tcctgccaag agttcttcgg gaggatccac gcgatcagcg gatacgcgat gaggccgagc
     4201 ccgaccgcga gcgggaagat gatgagcgac agcagcgcgg tcgatcgctc cagcgctttc
     4261 gcgcggcgat ccttcggcag ctcggcgatc gaaggcagca gcacgagcgc gatctgctcg
     4321 ccgacctgca ccgccggaat atcggcgagg ttgtaggcca tgttgtacgc gccgagatag
     4381 ccggtgccga agtagtacga gatggcgagg ttgtcccaat agcgcgacgc catatgcgcg
     4441 acgccctgaa ttccgagcgg cacaccaaac ccgagcatgt ccttcgtccg cttccacgac
     4501 agctgcgtcg gcgagaacca gctgcgcacg ccggccgcgc tgatgaggat ccacgtcgcg
     4561 acgatcgact tcacgatgtt cgcgacgatc atcgaccagc cgccccagcc atacgcggcc
     4621 atcggcagcg cggtcgcagt aaacgcgact tcgccaagcg cgagcgccat accgctcggc
     4681 cggaagttca tctgctgcgt gagcagtcgt tcgggcatcg cgccgagtcg ccggatgaag
     4741 acggcgatcg ccatgcccgg aatgtattgc gcggctttct cggcgttgaa gatcggcgca
     4801 aagaagccgc cgaacgcagc gacagcaccg agcgagatga tgccgagcac caagtagaag
     4861 aacgtggcgt gccagatgac ttctttacgg tcctcgccgc gccccttcac gacggtgtac
     4921 tgaccgaatc cccagatggt gatccagttc gcggtcatcg tgatgatcgc ggcgtccgac
     4981 acctcgccga tcacttccgg tgcgaggaag cgcgtgatga cgagcgtgcc gatgacgccc
     5041 accgcacgcc cacccatcga cgagatgatg gtccacaacg cgccacgtgc agctttgtga
     5101 gcgagggaca tgggaagtgg tgatgctctc gtgcagtccg catgccgcgc aagccatcga
     5161 tct
//
LOCUS       DATLBZ010000122         3144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10493937, whole genome shotgun sequence.
ACCESSION   DATLBZ010000122 DATLBZ010000000
VERSION     DATLBZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10493937"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VMZ53_06470"
     CDS             <1..1154
                     /locus_tag="VMZ53_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28132.1"
                     /translation="GSDGKPLNLVHRDVSSSNILVSYEGAIKLIDFGIARATSRTHQT
                     QVGTLKGKIPYMSPEQCRGLPLDRRSDLFSLGVVMYELTAGRRPFIGENDFQIMDQIV
                     HQGAQPPSSLMRGYPPELEAIVMKLLARKAEDRYQTAEEMLHDLDPFLQQHRLWVSPK
                     PLSRYMRQLFSTEVAAWDVALAEGASLEQHIAQTVVSSHSKQSELVTPPSSFPALMPL
                     SQEMPAVAMPTAGFSKVDAPVHVAPEPVPMPAPDLKRKNKIVFAAIGFVAVVAGGYAG
                     MTAIQNSKKPARAEAAPTPEPSQPIVMPQGPPVPPKPALETTPAVAAVPAKPVLEVEK
                     PKPKPATPVVKPKPKPRPTTTTTNTTTKPVDQPKDTGWDPNSPFLPPSK"
     gene            1115..1870
                     /locus_tag="VMZ53_06475"
     CDS             1115..1870
                     /locus_tag="VMZ53_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28133.1"
                     /translation="MGSKLAILAAIQVVLLAGSTVARAENKKLARELFELGIEEYKAK
                     DYPAAVASMSKSYSLDPQPNALYALAQAERLNDDCKNATLHYQKLLEVSKDDAIKKAV
                     AGNLELCAQIERGEKPKEETPAETKAAEQRDAPVIQYKTIYRTRTESKTDVLAVSMFA
                     VGGISLGGSVVTYLYARSTRSDADNATTLEDYNDKFDRAQRLRYVSYAAAGVGLALVG
                     VATFRVVRGSKKPTEVALIPTNGGTFFALSGSF"
     gene            complement(1867..3078)
                     /locus_tag="VMZ53_06480"
     CDS             complement(1867..3078)
                     /locus_tag="VMZ53_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS28134.1"
                     /translation="MDLFHDNPMYTAVRQMMGAFAKQSIRPISIKHDQEESMPWELMK
                     QAQAFGMTQTTLLDGRKKLTGVDEDPDPKKPRTQARLSVVASEEMAWGCAGIALALGG
                     SGLACAPVARMGTEEQKEEFRRLCKGLDEKGHVKVAAMALSEPATGSDISGLKTTARQ
                     DGDHWIIRGSKQWITNGQSAAVYVVWAQTDERAGRAGVRGFIVARGTPGLVPGKKETK
                     LGIRASETAQVHFEDVRVHKDMMLGVAQETNAGLADTKKMLDSTRPVVGSQAVGIARA
                     AYEFLLERVKGGSLHGTPMASHQHIMFELSEMQMEITASRLLAHRAAWLADHHLDNVA
                     EASMAKAHGARTAQSVTTRAMTLLGEEALEPGHPVEKWYRDAKIYDIFEGTGQIQRRI
                     IAKVMTGQNPT"
BASE COUNT          562 a         1064 c          995 g          523 t
ORIGIN      
        1 cgggctcgga tggcaagccg ctcaacctcg tccatcgcga cgtgtcgtcg agcaacatcc
       61 tcgtcagcta cgaaggcgcg atcaagctga tcgacttcgg catcgcgcgc gcgacgagcc
      121 ggacgcatca aacgcaggtc ggcacgctga agggaaagat cccgtacatg tcacccgagc
      181 agtgccgcgg gttgccgctc gatcgacgaa gcgatctgtt ctcgcttggc gtcgtgatgt
      241 acgagctcac cgcggggcgg cggccgttca tcggcgagaa cgatttccag atcatggatc
      301 agatcgttca tcagggcgcg cagccgccat cgtcgctcat gcgcggctac ccgccggagc
      361 tcgaagcgat cgtgatgaag ctcctcgcgc gcaaggccga ggaccggtac cagaccgcgg
      421 aagagatgct gcacgatctc gatccgttcc tgcagcagca ccggctgtgg gtctcgccca
      481 agccgctgtc gcgttacatg cggcagctgt tctcgacgga agttgcggca tgggatgtcg
      541 cgctcgccga gggcgcgtcg ctcgagcagc acatcgcgca gacggtcgtg tcatcgcaca
      601 gcaagcagag cgagctggtg acgccgccgt cgtcgttccc ggcgctgatg ccgctctcgc
      661 aggagatgcc ggcggtcgcg atgcccacgg ctggcttctc gaaggtcgac gcgcccgttc
      721 acgtcgcgcc cgaaccggtg ccgatgccgg cacccgacct caagcgcaag aacaagatcg
      781 tgttcgccgc gatcggcttc gtcgcagtcg tggcaggcgg gtacgccggg atgacggcga
      841 tccaaaacag caagaagccc gcgagggccg aagcagcgcc gacgccggaa ccgtcgcagc
      901 cgatcgtgat gccgcagggg cctcccgttc cgccgaagcc agcgctcgag acaacacccg
      961 ccgtcgccgc agtacctgcc aagccagtgc tcgaggtcga gaagccgaag ccgaagcccg
     1021 cgaccccggt cgtaaagccc aagccgaagc cgcgaccgac gacgaccacg acgaacacca
     1081 cgacgaagcc agtcgaccaa ccaaaggaca cgggatggga tccaaactcg ccattcttgc
     1141 cgccatccaa gtagtcctcc tcgcggggtc gaccgtcgcg cgcgccgaga acaagaagct
     1201 cgcgcgcgag ctgttcgagc tcggcatcga ggaatacaag gcgaaggatt atccggccgc
     1261 cgtcgcatcg atgtcgaagt cgtactcgct cgacccgcag ccgaacgcgc tctacgcgct
     1321 cgcgcaagcg gagcgtctga acgacgactg caagaacgcg acgctgcact accagaagct
     1381 gctcgaagtc tcgaaggacg atgcgatcaa gaaggcggtc gccggcaacc tcgagctgtg
     1441 cgcgcagatc gaacgcggcg agaagcccaa agaggagacg ccggccgaga caaaagcggc
     1501 ggagcagcgc gacgcgccgg tgatccagta caagacgatc tatcgcacgc gcacagagag
     1561 caagaccgac gtcctcgcgg tcagcatgtt cgccgtcggt ggtatctcgc tcggcggatc
     1621 ggtcgtgacg tatctgtacg cgcgctcgac gcgctcggat gccgacaacg cgacgacgct
     1681 cgaggactac aacgacaagt tcgatcgcgc acaacgtctg cgctacgtct cgtacgccgc
     1741 ggcgggtgtc ggtctcgcgc tcgtcggcgt cgcgacattt cgcgtcgtgc gcggaagcaa
     1801 gaagcccacc gaggtggcgc tgatcccgac gaacggcgga accttcttcg cgctgtcggg
     1861 ctcgttctag gtcgggttct ggcccgtcat caccttcgcg atgatgcggc gttggatctg
     1921 accggtgccc tcgaagatgt cgtagatctt cgcgtcgcgg taccacttct cgacggggtg
     1981 ccccggctcg agcgcttctt cgccgagcag cgtcatcgca cgcgtcgtaa cggactgcgc
     2041 ggtgcgtgcg ccatgcgcct tcgccatcga tgcttcggcg acgttgtcga gatgatgatc
     2101 ggcgagccat gcggcgcgat gcgcgagcag ccgtgacgcc gtgatctcca tctgcatctc
     2161 ggagagctcg aacatgatgt gctgatggct cgccatcggc gtcccatgca gcgagccgcc
     2221 cttgacgcgc tcgagcaaga actcgtacgc ggcgcgcgcg atgccgaccg cttgtgagcc
     2281 gacgacgggc cgcgtcgagt cgagcatctt cttcgtatcg gcgagacctg cgtttgtctc
     2341 ctgcgcgacg ccgagcatca tgtccttgtg cacgcggacg tcctcgaagt ggacctgcgc
     2401 ggtctccgat gcgcggatgc cgagcttcgt ctccttcttg ccgggcacga ggccaggcgt
     2461 tccacgcgca acgatgaagc cgcgcacgcc ggcgcgacct gcacgctcgt cggtctgcgc
     2521 ccacacgacg tagaccgccg cggactgacc gttcgtgatc cactgcttgc tgccgcggat
     2581 gatccaatga tcgccgtcct ggcgcgcggt cgtcttcaag cccgagatgt ccgagccggt
     2641 cgcgggctcc gagagcgcca tcgccgccac tttgacgtgg cccttctcgt cgaggccctt
     2701 acacagccgg cgaaactctt ccttctgctc ctcggtgccc atgcgcgcga cgggcgcaca
     2761 cgcgagcccc gagccgccga gcgcgagcgc gatacccgcg cagccccacg ccatctcttc
     2821 gctcgcaacg acagacagcc gcgcttgcgt cctcggcttc ttcggatcgg gatcctcgtc
     2881 gacgccggtc agcttcttgc gtccatcgag cagcgtcgtc tgcgtcatgc cgaatgcctg
     2941 ggcttgcttc atcagctccc acggcatcga ctcttcttga tcgtgcttga tcgagatcgg
     3001 gcgaatcgat tgcttcgcga atgcgcccat catctgacgg acggcggtgt acatcgggtt
     3061 gtcgtggaag aggtccatta ctgcaccgcc ttctgcgcga ggacgcggcc gagccgctca
     3121 tcaccatgca gcgcttcgaa cgct
//
LOCUS       DATLBZ010000123         5131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_120843, whole genome shotgun sequence.
ACCESSION   DATLBZ010000123 DATLBZ010000000
VERSION     DATLBZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120843"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VMZ53_06485"
     CDS             <1..338
                     /locus_tag="VMZ53_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28135.1"
                     /translation="LDGDGEHAVMLDPSWNDGLRDFLGRGPERGEDIVEAWLLDDPYR
                     TFTIIYSTRSVGWANYVALQKRESVGDRVKVCRVSEPHFLVPTAKGIHDALVDPVEWD
                     NGTCTWGAP"
     gene            complement(339..1346)
                     /locus_tag="VMZ53_06490"
     CDS             complement(339..1346)
                     /locus_tag="VMZ53_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28136.1"
                     /translation="MRRVATAALPLLLLALGACGDNEPACGHVKVLVGGRNVWSPIFA
                     VDEQHMYYADYDVDGYGTKLLLRASRDGGGLQSLGQVADYEMFGNGIALDDRNVYWTA
                     SRFLDNLGYSLYASPRAGGSPFPIANLPPCVPFGVTSSTTEVFAGTSACDAQPSRVTA
                     VDKTTSNARIAWEAGMNDGDVRALAYGGDTLFIATEIALFAVRPSATEVVTAGNTIRH
                     LEVHDDFLYYAEEHDGVYRTPLAGGTRELLYAYAPTGERQGAFSLDGDDLYVADPPHM
                     MFVPLATRTPQAIVHDVGSLSEISARDGYAWWSSLVLPAAPGGLDTFSGGIERVARPC
                     D"
     gene            1371..1835
                     /locus_tag="VMZ53_06495"
     CDS             1371..1835
                     /locus_tag="VMZ53_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28137.1"
                     /translation="MSGSKAKLKAELLIALEAQLAAARSAHEAAVAGATHEEAKAEND
                     KDTRGLEQSYLARGHAQRVGELENAVAVTQVFAPRAFGESDAIALGALIDIEEDGKAK
                     KLWLAPFGGGVELVGGITVVTPTSPLGTALLGKRVDDDVELGKRVLTVNGIE"
     gene            1855..2700
                     /locus_tag="VMZ53_06500"
     CDS             1855..2700
                     /locus_tag="VMZ53_06500"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HUS28138.1"
                     /translation="MSVFLFGATSMLGWSILRAAGPEEPTAFRNALTKAEVEGISAGI
                     DLDDEPSVRELFAREKPRLIINCAGVCDVGTCEQSPDFAHVVNVEGTRVLLAHAPPDA
                     RIVHCSSDHVFSGDGGPYDESTPTDPISVYGRTRVEAEQLVLARPNTLVIRAGLWIGP
                     SSTGRIGHLDWLRDRTKRGLPMTIVADEHRSAVWAEDAARRVWELARSSITGVRHIVA
                     SRIVSRPELARFLDEKFGIGAKFDTETRAQRWERAATPHLGKVSLVTRFTDALAAPLP
                     PVVPA"
     gene            2697..3842
                     /locus_tag="VMZ53_06505"
     CDS             2697..3842
                     /locus_tag="VMZ53_06505"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28139.1"
                     /translation="MKCTSCATELDSRATFCPSCGATVPDPFIGMAVSGRYEIAKRIA
                     IGNFGSIYRGTDNVNGKEVALKIMHRELAADTRLVERFRREGVVLRKLLSRNVPATYE
                     LGEADGLPFIAMELLQGDTLLGLFRSNGRLPWQKVFAYGIQVCSALDEAHRLGIIHRD
                     LKPANIFVSDGDVVKVLDFGIAKILSSSDIPNPQELTIMGTAVGTLEYMAPEQLMGGR
                     ADGRTDIYTLGVVMYELITGHRPFNVAGLELLTIQLSEQPPPPSADVDVPPEVDEILL
                     RCLKEDLDDRYPNVSELARALDEPLSAGDFVPTPAPWAKPAATAAAPAPQPLSTVQPS
                     RPAPAFPMTPPMVQDEPVGRIAPAKTPYVIVAVIVLAAIIAIALIAK"
     gene            3852..4190
                     /locus_tag="VMZ53_06510"
     CDS             3852..4190
                     /locus_tag="VMZ53_06510"
                     /inference="COORDINATES: protein motif:HMM:NF022731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3088 family protein"
                     /protein_id="HUS28140.1"
                     /translation="MKDTLFLLRPGFTDKGKTYFCPYSAQVVGFLAYYPQVRESLDVI
                     ELDFTKPREPLATMLGAEHQAAPMLVLGGPPVDVPDVKIKEERGHSYVEKTIEILRYL
                     AATRSVPLPH"
     gene            complement(4192..>5131)
                     /locus_tag="VMZ53_06515"
     CDS             complement(4192..>5131)
                     /locus_tag="VMZ53_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28141.1"
                     /translation="CACGDDGFSPMGDGGGVDAVDAAPDTPDAPMIDMPAIPTGCVAG
                     RVTADRPDDSAFDQIRALYVLPSDAVDRQFDTNDKICNSLRGVSAWFHTQSNVYFRLD
                     TSGDLIDVGFVRLNKTDAQMRGTDTTNTSIDTGTAFVRERIERELKAMGLIASNKLYA
                     VYYEGSSVYACGGGAYPPLIKARVGAVYLRGMPPGVTVPCGDSFPWGQPSLVPSYIDY
                     AILHELVHSMGIVPTTSPNEHAQGHVFDTASTTPARDLMYSPRTSGDPAWATNNPSGL
                     LIDINRDDYFNAGTVDLATMSLISPLPSTPHRPNGW"
BASE COUNT          856 a         1671 c         1753 g          851 t
ORIGIN      
        1 gcctcgacgg cgatggtgag catgcggtca tgctcgatcc gtcgtggaac gacggtctgc
       61 gcgacttctt gggtcgtggg cccgagcgcg gcgaggacat cgtcgaggcg tggttgctcg
      121 acgatccgta tcggacgttt acgatcattt acagcacacg cagcgtgggg tgggcgaact
      181 acgtcgcgct gcagaagcgc gagagcgtcg gcgatcgcgt gaaggtctgt cgcgtcagcg
      241 agccgcactt tctcgtgccg accgcgaagg gcatccatga tgcgctcgtc gatcccgtcg
      301 agtgggacaa cggcacgtgc acgtggggcg cgccgtaatc agtcgcaggg ccgcgcgacg
      361 cgctcgatgc cgccggagaa tgtgtcgagg cccccgggcg cggcgggaag gacgagcgag
      421 ctccaccacg catagccatc gcgcgcggag atttcgctga gcgagccgac gtcgtgaacg
      481 atcgcttggg gcgtgcgcgt cgccagaggt acgaacatca tgtgcggcgg gtcggccacg
      541 tagaggtcgt caccgtcgag ggagaatgcg ccttggcgtt cgccggtggg ggcgtaggcg
      601 tagaggagct cgcgcgtgcc gccggcgaga ggtgtgcggt acacgccgtc gtgttcttcg
      661 gcgtagtaga ggaagtcgtc gtgcacttcg aggtggcgga tcgtgttgcc cgctgtgacg
      721 acttcggtgg ctgacgggcg cacggcgaac agcgcgatct cggtcgcgat gaagagggtg
      781 tcgccgccgt aggcgagcgc gcgcacgtcg ccgtcgttca tgcctgcttc ccacgcgatg
      841 cgagcgttgc tcgtggtctt gtcgacggca gtgactcgcg atggctgggc atcgcacgcg
      901 gacgtgccgg cgaagacttc ggtggtgctc gacgtcacgc cgaacggtac gcatggcggc
      961 aggttcgcga tcgggaatgg cgagccgcct gcgcgcggcg acgcgtagag cgagtagccg
     1021 aggttgtcga ggaagcgtga tgccgtccag tagacgttgc gatcgtcgag cgcgatgccg
     1081 ttgccgaaca tctcgtagtc tgcgacttgg ccgagcgatt ggaggccgcc gccatcgcgc
     1141 gaggcgcgga gcaggagctt cgtgccgtag ccgtcgacgt cgtagtcggc gtagtacatg
     1201 tgttgctcgt cgaccgcgaa gatcggcgac cagacgttgc gcccaccgac gaggactttc
     1261 acgtggccgc acgccggctc gttgtcgccg cacgcgccga gcgcgagcag cagcagcggc
     1321 agggcggcag tggcgacgcg gcgcatggat gcaagatact ctgccttgcg atgtctggct
     1381 cgaaggcgaa gctcaaggcc gaactgctga tagcgctcga ggcgcagctc gcggcggcgc
     1441 gttcggcgca cgaggccgcg gtggccggcg cgacgcacga agaggcgaag gccgaaaacg
     1501 acaaggacac gcgcggcctc gagcagtcgt atctcgcgcg tgggcacgcg cagcgcgtcg
     1561 gcgagctcga gaatgcagtg gccgtgacgc aggtgtttgc gccgcgcgcg tttggcgaga
     1621 gtgatgcgat cgcgctcggt gctctcatcg acatcgagga ggacggcaag gcaaagaagc
     1681 tgtggctcgc gccatttggc ggcggggtcg agctggtggg tgggatcacc gtcgtcacgc
     1741 cgacgtcgcc gctcggcacg gcgctgctcg gcaagcgcgt cgacgacgat gtcgagctcg
     1801 gcaagcgggt gctcacggtc aacggcatcg aatgagatgg gctatgctcg ccgcgtgagc
     1861 gtgttcctgt tcggagcgac ctccatgctt ggatggtcga tcctgcgcgc ggcggggccc
     1921 gaagagccga ccgcgttccg caatgcgctc acgaaggccg aggtcgaagg catcagcgcc
     1981 ggcatcgatc tcgacgacga gccgagcgtc cgcgagctgt tcgcgcgcga aaagccgcga
     2041 ctgatcatca actgcgctgg cgtgtgcgac gtcggcacgt gcgagcagtc acccgacttc
     2101 gcgcatgtgg tgaacgtcga agggacgcgc gtcttgctcg cgcatgcgcc tcccgatgcg
     2161 cgcatcgtgc attgctcgag cgatcacgtg ttctccggcg acggcgggcc gtacgacgag
     2221 agcacgccga ccgatcccat tagcgtctat ggacgcacgc gcgtcgaggc ggagcagctc
     2281 gtgctcgcgc gtcccaacac gctcgtgatt cgtgcgggct tgtggatcgg cccgtcgtcg
     2341 acagggcgca tcggccacct cgattggctg cgcgaccgca cgaagcgcgg gctgccgatg
     2401 acgatcgtcg cggacgagca tcgctcggcg gtgtgggccg aagatgcggc acgccgcgtc
     2461 tgggagctcg cgcgttcgag catcaccggc gtccggcaca tcgtcgcgtc gcgaatcgtc
     2521 tcgcggcctg agctcgcgcg attcctcgac gagaaattcg gcatcggcgc gaagttcgac
     2581 accgagacgc gcgcacaacg ctgggagcgc gctgctacgc ctcacctcgg taaggtctca
     2641 ctcgtcacgc gcttcaccga tgcgctcgcc gcacctttgc cgcccgtcgt gcccgcatga
     2701 agtgtacgag ctgcgcaacc gagctcgact ctcgcgcgac gttctgtccg agctgcggcg
     2761 ccacggttcc cgatccattc atcggcatgg cggtgagcgg ccgctacgag atcgcgaagc
     2821 gcatcgcgat cggcaacttc ggatcgatct atcgcggcac cgacaacgtg aacggcaaag
     2881 aggtcgcgct caagatcatg catcgcgagc tcgccgctga tacgcgcctc gtcgagcgct
     2941 tccggcgcga gggcgtcgtc ctgcgcaagc tgctctcgcg caacgtgccc gcgacgtacg
     3001 agctcggcga agccgacggc ctgccgttca tcgcgatgga gctcttgcag ggcgacacgc
     3061 tgctcggcct gtttcgcagc aatggccggc tgccgtggca gaaggtcttc gcgtacggca
     3121 tccaggtctg cagcgcgctc gacgaagcgc accgcctcgg catcattcat cgcgatctga
     3181 agcccgccaa tatctttgtc tccgacggcg acgtcgtgaa ggtcctcgac ttcggcatcg
     3241 cgaagattct ctcgagcagc gacattccca atccgcagga gctcacgatc atgggcaccg
     3301 cggtcggcac gctcgagtac atggcgcccg agcagctcat gggcggacgc gcggatggac
     3361 gcacggacat ctacacgctc ggcgtcgtca tgtacgagct catcaccggc catcgcccgt
     3421 tcaacgttgc tggcctcgag ctgttgacga tccagctcag cgagcagccg ccacctccgt
     3481 cggcggacgt cgacgtccca cccgaagtcg acgagatcct gctgcgctgc ctcaaagaag
     3541 atctcgacga tcgctacccg aacgtcagcg agctcgcgcg cgccctcgac gagccgctct
     3601 cggcgggaga cttcgtgccg acgccagcac cgtgggcgaa acccgcggcc accgctgcag
     3661 cgccggctcc ccagccgctg tcgacggtgc agccctcgcg ccccgctccc gcgttcccga
     3721 tgacgccgcc gatggtccaa gacgagcccg tcggccgcat cgcgccggca aagacgccct
     3781 acgtgatcgt tgccgtgatc gtcctggccg caatcatcgc catcgcgctg atcgcgaagt
     3841 agccttcggt catgaaggac acgctgttcc tgctgcggcc cggcttcacg gacaagggca
     3901 agacatactt ctgcccgtac agcgcgcagg tcgtcggttt cctcgcgtac tacccgcagg
     3961 tgcgcgagag cctcgacgtg atcgagctgg acttcacgaa gccgcgcgag cctctcgcca
     4021 cgatgctcgg cgccgaacat caagcggcac cgatgctcgt cctcggcggc ccgcccgtcg
     4081 atgtccccga cgtcaagatc aaggaagagc gcggccactc gtacgtcgaa aagacgatcg
     4141 agatccttcg ctacctcgcc gcgacgcgca gcgtaccgct accgcactga ctcaccagcc
     4201 gttggggcgg tgcggcgtgc tcggcagcgg gctgatcaac gacatcgtcg cgagatcgac
     4261 ggtgcccgcg ttgaagtagt cgtcgcgatt gatatcgatc aaaaggcccg acggattgtt
     4321 cgtcgcccac gccggatcgc cggacgtccg cggcgaatac atgaggtcgc gcgcaggcgt
     4381 cgtcgaggcc gtgtcgaaca cgtggccttg tgcgtgctcg ttcggcgagg tcgtcggcac
     4441 gatgcccatc gagtgcacga gctcgtgcag gatcgcgtag tcgatgtagc tcggcacgag
     4501 gctcggctgt ccccacggga acgaatcgcc gcatggcacc gtcacgcccg gcggcatgcc
     4561 gcgcaagtac accgcgccga cgcgcgcttt gatcagcggc ggatacgcac caccgccaca
     4621 cgcgtagacg ctcgagcctt cgtagtagac cgcgtagagc ttgttcgatg cgatgaggcc
     4681 catcgccttg agctcgcgct cgatccgctc gcgcacgaat gcggtgccag tatcgatgct
     4741 cgtgttggtc gtgtccgtac cgcgcatctg cgcgtcggtc ttgttcagcc ggacgaagcc
     4801 gacatcgatg aggtcgccgc tcgtgtcgag ccggaagtaa acgttcgact gcgtgtggaa
     4861 ccacgccgac acgccgcgca gcgagttgca gatcttgtcg ttcgtatcga actgccgatc
     4921 gaccgcatcc gatggcagca cgtagagcgc gcgaatctga tcgaacgcgc tgtcatcggg
     4981 gcgatcggcg gtgacccgac cagccacgca gccggtcggg atcgccggca tgtcgatcat
     5041 tggtgcatcg ggggtgtcgg gcgcagcgtc caccgcgtcg acgccgccac catcgcccat
     5101 cggagagaag ccatcgtcac cgcacgcgca c
//
LOCUS       DATLBZ010000124         5393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_866014, whole genome shotgun sequence.
ACCESSION   DATLBZ010000124 DATLBZ010000000
VERSION     DATLBZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866014"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VMZ53_06520"
     CDS             complement(<1..1032)
                     /locus_tag="VMZ53_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUS28142.1"
                     /translation="MTTSGSPSSQTTQVITLLRWLLIAVFVVVGWLTLSYMAKVLAPI
                     LAALGIAYLLNPVLERLVKAGMSRPLGAGILLVLFVGLIVVGLVTAVSAIVHQVQDVA
                     HDLPRMITNLDVWLKDHFDFGLPEDWQSYLTKENLQHMATGGPARAFATAAVGGVLAF
                     VAHAAEFLLVPVFAFYFLVDWPNMLRRIETLVPPRKRRGIREMVAEIDRVVAGWVRGQ
                     AIVTSILAVLYAIGFTIVGMPLSVPIGIAVGLLTVIPFVGTLVGALIAAAVTIAGGGS
                     LATLGEVGVVILVLHLLEAGVLTPKIVGHRVGLSESGALFAVVAGGKLLGFVGVVLAV
                     PIAATVAVLV"
     gene            complement(1052..1729)
                     /locus_tag="VMZ53_06525"
     CDS             complement(1052..1729)
                     /locus_tag="VMZ53_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HUS28143.1"
                     /translation="MQAPGLLKDGYRVGQAIVRVLGDSTKTHEIHRVEEITGRPRYRQ
                     LQREMEATSEGRRLLAERPELSSDHVDYDHLRTLPESTLGGAYVHHLDSHGLSADYQA
                     AATRHVDDPTMAYLMRRFRQTHDVWHALLGLGVEGYEEVLVHWFTFGQLKLPVSAMIM
                     LFGSMKHIVLEQRWGAMRHSMMEAYRAGRDAEPLLPVYWEDHWEQPIEEIRARYNVRP
                     LERRWLE"
     gene            1850..3913
                     /locus_tag="VMZ53_06530"
     CDS             1850..3913
                     /locus_tag="VMZ53_06530"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HUS28144.1"
                     /translation="MSRRTLLLVSLSLPLSLLAACSDDDTGSAPSARLLTMADCGAVK
                     DYMKTVAIEQMNRRIDDELAAYIRGERCYRGYGEDDSVGQPSPGSGTGGGSGGNSGPT
                     TGTGTNNQVAGVDEADFIKNDGQYIYLAQNGVFRIVDSWPADLTHEVSATPVEGEPKK
                     LFVLGDKALVYVATGSANNGYYNRECTYGYTCDFTGDGTGTKLLVFNISDRTAPVLER
                     EVSLSGSLIAGRRVGTAVHTVVTQAANPFPSLDYNPDEELCNYQGAKPTAYNVLLAKQ
                     AYDRLRATNRETIMATDLEQKLPTLADSAGLTPDGEAASCESLYASSINDGSAFTSIV
                     SVDLTHPGPVKTSTIVSAPGAIYASEDALYMAVPHRWTSGGWGGYDTTDSVSTVHKFR
                     ISEIAGQTKYLASGLINGRVLNQFAMDEKDHLLRIASTDGHVPNPETNSYLTVLEEQH
                     DELVAIGKIGGIAPDEDIRSVRFDGNHAFVVTFKKTDPLFAFDLSDPTDPEMVGELQI
                     PGFSTYMHLIDSTHLLTIGYDADDHGDFAFFDGIQLQIFDVSNMASPQLASRYLIGTR
                     GSSSEALTNHLAFTWYPEKGLLSLPMTICEGGGDGTYGTNMTFSGLMVFDASIASGFS
                     EHGRVPHTVSPDATCSNWWTNASSAVKRSLFLDNFVYSVSDSELRVRDVNAMSTELVT
                     LDLTP"
     gene            complement(3910..4215)
                     /locus_tag="VMZ53_06535"
     CDS             complement(3910..4215)
                     /locus_tag="VMZ53_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28145.1"
                     /translation="MKRAALLLALLAACNHNKAGTSRVTADDAIFYVKSNVPDAGLYV
                     DGRFVGPVGGLKGGVAVVAGKHRLEVRHDDYFARYIELDLQRAERKRLDIDLAPVLP"
     gene            complement(4212..>5393)
                     /locus_tag="VMZ53_06540"
     CDS             complement(4212..>5393)
                     /locus_tag="VMZ53_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28146.1"
                     /translation="GVKRCVLALVLMAAAAATPAFAEDIVAYQAEGDAPVSATDPRLM
                     ALDEAFGRAVNSALVDLVEGDVRTARKGELDREIVGHARLWVAKYAVTKDETVDDRRE
                     LTVSVRIDRDKIRGKLGELKIAVKEAATTQTPATPDTQPQAKTITILLRIVMPTGTKA
                     DYGAAADANAPGVSALTTLLRGAGFAVRRAPASGDVKDALSDDDAAGLAAEAKAEHVA
                     VASVMVGDQVPVRGLPTTAALVSAELRILDKSKNVVGRASSSTAAKGDETAYAVDRAL
                     LAAAADVLPPAPTKLSAAGAYQGDDTPIVEPGIVLVRLPARTPYALVLAEQKYLAGAK
                     GVKAATLRRLSPSGWVIGVTTTESIETVARIAKKAPASDTTSAVKIAGGVVEVSLAGS
                     P"
BASE COUNT          974 a         1854 c         1705 g          860 t
ORIGIN      
        1 cacgaggacg gcgaccgtcg ctgcgatcgg caccgcgagc accacaccga caaagcccag
       61 cagcttgccg cccgcgacga cggcgaacag cgcgcccgat tcggagaggc cgacgcggtg
      121 gccgacgatc ttcggcgtga ggacacccgc ctcgagcaag tggagcacga ggatcacgac
      181 gccgacctcg ccgagcgtcg cgagcgaacc gccaccggcg atcgtcacgg ctgccgcgat
      241 caacgcgccg acgagcgtgc cgacgaaggg aatcacggtg agcagaccga cggcgatgcc
      301 gatcggcaca gacagcggca tgccgacaat cgtgaaaccg atcgcgtaca gcaccgcgag
      361 gatcgaggtc acgatcgcct gaccgcgcac ccatcctgcg acgacgcgat cgatctcggc
      421 caccatctcg cgaatgccgc gtcgcttgcg tggcggaacc agcgtctcga tccgccgcag
      481 catgttcggc caatcgacga ggaagtagaa cgcgaacacg gggacgagca agaactccgc
      541 tgcatgtgcc acgaacgcga gcacaccgcc gacggccgca gtcgcgaacg cgcgagcggg
      601 tccgcccgtc gccatgtgct ggagattctc tttcgtcaga tagctctgcc agtcctccgg
      661 cagcccgaag tcgaagtgat ccttcagcca cacgtcgagg ttcgtgatca tgcgcgggag
      721 gtcgtgcgcg acgtcctgca cctgatggac gatcgcggac acggccgtca cgagaccgac
      781 gacgatcagg ccgacgaaca gcacgagcaa gatgcccgca ccgagcgggc gcgacatgcc
      841 ggctttgacg aggcgttcga ggaccgggtt gagcagatac gcgatgccta gcgcggcgag
      901 gatcggcgcg aggaccttcg ccatgtagct cagcgtcagc cagccgacga cgacgaacac
      961 cgcgatgaga agccaacgca ggagagtgat gacctgcgtg gtctgtgacg atggcgatcc
     1021 cgaagtcgtc atcggatccg ctgtatcacg attattcgag ccagcggcgt tccagcggcc
     1081 gaacgttgta gcgagcgcgg atctcttcga tcggctgctc ccagtggtct tcccagtaga
     1141 cgggcagcag tggctcagca tcgcgacctg cgcgatacgc ctccatcatc gagtgccgca
     1201 tcgcgcccca ccgctgctcg agcacgatgt gcttcatgct gccgaacagc atgatcatcg
     1261 cgctcaccgg cagcttcagc tggccgaacg tgaaccagtg cacgagaacc tcttcgtaac
     1321 cctcaacgcc gaggccgagc aacgcgtgcc agacatcgtg ggtctgccga aaccgccgca
     1381 tgaggtacgc catggtcgga tcgtcgacgt ggcgcgtagc cgctgcttgg tagtccgccg
     1441 agaggccgtg gctgtcgaga tgatgaacgt atgcaccgcc gagcgtggat tccggcagcg
     1501 tccgcaaatg atcgtaatcg acgtgatcgc tcgagagctc ggggcgctcg gcgaggagcc
     1561 gcctgccttc ggacgtggct tccatctcgc gttggagctg gcggtatcgc ggccgccccg
     1621 tgatctcctc gacacgatgg atctcgtgcg tcttcgtcga gtcgccgagc acgcgaacga
     1681 tcgcctgccc tacgcgataa ccgtccttga gaagacccgg cgcctgcatg actgcagcgt
     1741 accagcctcg gatttttgag accgctcacg gatctccgaa tcgccgattc cataagccac
     1801 cgtgatgttt ggtacccgcg cgggcactgg gcttgctgtg gtggagcgta tgtcccgtcg
     1861 caccctcctg ctcgtgtccc tgtcgttgcc cctctcgcta ttggctgcct gctccgatga
     1921 cgacaccggc agcgcgccgt ccgcccgcct cctcacgatg gccgattgcg gcgccgtgaa
     1981 ggactacatg aagacggtcg cgatcgagca gatgaaccgc cgcatcgacg acgagcttgc
     2041 cgcatacatc cgcggcgagc gctgctatcg cggctacggc gaagacgaca gcgtcggaca
     2101 accgtcaccc ggcagcggca cgggcggtgg cagcggcggc aacagcggcc cgacgaccgg
     2161 cacgggcacg aacaaccagg tcgccggcgt cgacgaagcc gacttcatca agaacgacgg
     2221 ccaatacatc tacctcgcgc aaaacggcgt gttccgcatc gtcgattcgt ggcccgcgga
     2281 tctcacccac gaagtcagcg cgacacccgt cgagggcgag ccgaagaagc tgttcgtgct
     2341 cggcgacaaa gcgctcgtct acgttgccac cggcagcgcg aacaacggct actacaaccg
     2401 cgagtgcacg tacggctaca cgtgcgactt caccggcgac ggcacgggca cgaagctcct
     2461 cgtgttcaac atcagcgatc gcaccgcgcc ggttctcgag cgcgaagtct cgctctctgg
     2521 ctcgctgatc gcaggacgtc gcgtcggcac cgcggtgcac accgttgtca cgcaagcggc
     2581 caatccattc ccctcgctcg actacaaccc cgacgaggag ctctgtaact accaaggcgc
     2641 gaagccgacc gcatacaacg tgctgctcgc gaagcaggcg tacgacaggc tgcgcgccac
     2701 caatcgcgag acgatcatgg cgaccgatct cgagcaaaag ctgccgacgc tcgccgacag
     2761 cgcgggcctc acgcccgacg gtgaagccgc cagctgcgag tcgctctacg cgagctcgat
     2821 caacgacggc tcggcattca cgagcatcgt cagcgtcgac ctcacgcacc cgggaccggt
     2881 gaagacgtcg acgatcgtct cggcgccggg cgccatctac gcatccgaag acgcgctcta
     2941 catggccgtc ccgcatcgct ggacttccgg cggttggggc ggctacgaca cgaccgattc
     3001 ggtctcgacc gtgcacaagt tccgcatctc cgagatcgcc ggccagacga agtacctcgc
     3061 gagtggcctc atcaacggcc gcgtgctgaa ccagttcgcg atggacgaga aggatcacct
     3121 gctccgcatc gcgagcaccg acggccacgt cccgaatccc gagacgaaca gctacctgac
     3181 cgtgctcgaa gagcagcacg acgagctggt cgcgatcggc aagatcggtg gcatcgcccc
     3241 cgacgaggac atccgctcgg tccgcttcga cggcaaccac gcgttcgtcg tgacgttcaa
     3301 gaagaccgat ccgctgttcg cgttcgacct ctccgatccg acggacccgg agatggtggg
     3361 cgagctgcaa atccccggct tctcgacgta catgcacctg atcgactcga cgcacctcct
     3421 gacgatcggc tacgacgccg acgatcacgg tgacttcgcg ttcttcgacg gcatccagct
     3481 gcaaatcttc gacgtgtcga acatggcgtc gccgcagctc gcgtcgcgct atctgatcgg
     3541 cacgcgcggc tcgagctccg aagcgctgac gaaccacctc gcgttcacgt ggtacccgga
     3601 gaaagggctg ctctcgctgc cgatgacgat ctgcgaaggc ggcggtgacg gcacgtacgg
     3661 cacgaacatg acgttctcgg gactgatggt gttcgacgcg agcatcgcga gcggcttctc
     3721 ggagcacggc cgcgttccgc acacggtctc gccagacgcg acgtgctcga actggtggac
     3781 caacgcgagc agcgcggtca agcgctccct cttcctcgac aacttcgttt actcggtctc
     3841 ggatagcgag ctgcgcgtgc gcgacgtcaa cgcgatgtcg accgagctcg tgacgctcga
     3901 cctgacgccc taaggcagaa ccggcgcgag atcgatatcg agtcgcttgc gctcggcgcg
     3961 ctggaggtcg agctcgatgt agcgcgcgaa gtaatcgtcg tggcgcacct cgaggcgatg
     4021 cttgcccgcg acgaccgcga cgccgccctt gaggccaccg accggaccga cgaagcggcc
     4081 gtcgacgtag aggccggcat ccggaacgtt ggacttcacg tagaaaattg cgtcgtcggc
     4141 ggtgacgcgg ctcgtgcccg ccttgttgtg gttgcaggcg gcaagcagcg ctaggagcaa
     4201 cgccgcgcgc ttcatggcga gcctgcgagt gagacctcga cgacaccgcc ggcgatcttc
     4261 acagcgctcg tggtgtcgct tgccggtgcc ttcttcgcga tgcgcgcaac ggtctcgatc
     4321 gactcggtcg tcgtgacgcc gatgacccag ccgctcggcg agagccggcg cagcgtcgcg
     4381 gccttaacgc ccttggcacc ggcgagatac ttctgctcgg cgagcacgag cgcgtacggc
     4441 gtcctcgccg gaaggcgaac gagcacgatg cccggctcga cgatcggcgt gtcgtcgcct
     4501 tgataggcgc ccgcggcgga cagcttcgtc ggtgcggggg gcagcacatc ggccgctgcc
     4561 gcgagcaatg cgcgatcgac cgcgtatgcc gtctcgtcac ccttcgcggc ggtcgagctg
     4621 ctcgcacgtc cgacgacgtt cttcgacttg tcgaggatgc gcagctccgc gctgacgagc
     4681 gctgcggttg tcggcaggcc gcggacaggc acttgatcgc ccaccatcac gctcgcgacg
     4741 gcaacgtgct cggccttcgc ttcggcggcg aggcccgccg cgtcgtcgtc ggacagcgca
     4801 tctttcacgt cgcccgatgc gggcgcacgt cgaacggcaa agccggcgcc gcgcaacagc
     4861 gtcgtcagcg cgctcacgcc cggtgcattc gcatcggccg ctgcgccgta gtccgccttg
     4921 gtcccggtcg gcatcacgat gcggagcaga atcgtgatcg tctttgcctg cggctgcgtg
     4981 tcgggggtcg cgggcgtctg cgtcgtcgcc gcttccttca ccgcgatctt cagctcgccg
     5041 agcttcccgc ggatcttatc gcggtcgatg cggacgctga ccgtgagctc gcggcggtcg
     5101 tcgaccgtct cgtccttcgt gaccgcatac ttcgcgaccc acagacgcgc gtggccgacg
     5161 atctcgcgat cgagctcgcc cttgcgcgcc gtccgcacgt cgccctcgac gagatcgacg
     5221 agcgcgctgt tcacggcacg gccaaacgct tcatccagcg ccatcaggcg tgggtcggtc
     5281 gcgctcaccg gcgcatcgcc ttccgcttga tacgcgacga tgtcctcggc gaacgctggc
     5341 gtcgcggcag ctgcagccat cagcacgaga gcaaggacac accgcttcac gcc
//
LOCUS       DATLBZ010000125        48122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9751538, whole genome shotgun sequence.
ACCESSION   DATLBZ010000125 DATLBZ010000000
VERSION     DATLBZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 48122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48122
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9751538"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1727)
                     /locus_tag="VMZ53_06545"
     CDS             complement(72..1727)
                     /locus_tag="VMZ53_06545"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HUS28147.1"
                     /translation="MRTIVIAMLAAAACGDLDYKMPDAPPPDTCNAVTWYADCDGDGV
                     AAMTSDTTMSCTMPAAPACGGTWVTAMPVAGATDCNDANAAIKPSATEICDLQDNNCN
                     GQTDETGLTTFYRDMDSDGHGSLSMSMTTCGAPTGYVTSADDCDDTNGAIHPGAAEAC
                     NGVDDNCAAGIDEGVKMTFYQDGDGDGYGTSAATNQQCAAGGGYVANATDCNDGNGAI
                     HPGVAEVCNGVDDNCAGGADEGVTSTFYLDNDGDGYGNAGASQQRCAAGGGYIANATD
                     CNDGNGNIHPNAAEVCNFVDDNCVNGIDEGVQTLWYADGDGDGYGAGTATAACSSPPV
                     AGYVSNNTDCNNGNGNVHPNATELCFNGVDDNCVNGQDDGARCSMDCDWIGTRWLSWG
                     YDGDPASVGVWATCTNGKLAGMSYENKSGQTQSLAALSPPQVTGGTSTDYIDCDWGNA
                     NRWASQGWNAGSAFTFGDHIHCDGTRVSSMSWSSNNALLPGQIQHADIAGQLSCDWNG
                     GIFLSYGFDGSCAFGAGFVVTCSNNHITNFHWFEGGACIHTVE"
     gene            complement(1806..2675)
                     /locus_tag="VMZ53_06550"
     CDS             complement(1806..2675)
                     /locus_tag="VMZ53_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28148.1"
                     /translation="MSEAFASALDVSARPRFEGNVGIADTLAQLRAAATAAFPEIAVT
                     ETEYASELARRLGAAATPELLARVRADHVHLAIACARGDEAAIRRFESEFLDEVAACA
                     QRMRARDDQADEVRSYLRRTLFVSEPGRNAALSEYSGRGDLRSYLRVITTRELVRVID
                     GGKREVGIEEESFFDKLSPLSDPEVGYLREAYRPDVDAAMRIAVSKLAADSRALLRYS
                     LVDGWSIDRIAKLYGIHRATAARRVAAVRDELGELMRIELAARLAIPVEEVDSVVRLV
                     QSRVDVSLSRLLG"
     gene            2728..4866
                     /locus_tag="VMZ53_06555"
     CDS             2728..4866
                     /locus_tag="VMZ53_06555"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28149.1"
                     /translation="MECANADTLAGLVAGALPEAERAAIATHAASCKRCHALIDELFE
                     SSSAGGPVTPAGPDDTTQGDEPDGSRPSRISLRPGAHVGRYVIEERIGAGGMGVVFAA
                     TDSELHRRVAIKLLRQGGQGHARMSTAGRERLMREARLLASLSHPNVLTVFDVGTHET
                     DVFIAMELVDGGSLSSWLRRAPRTTDEIIDRMIEAGRGLGAAHAGGVVHRDVKPDNIL
                     VGLDGRARVTDFGLARIDQELAPDIDAMRAAALSPDLTKTGALMGTPVYMAPEQWRKG
                     AVDPRTDQWSFCATLYELVAGVRPFTVDDPDKRAAEIEEGRIAAPADGRTVPSWLRKI
                     VERGLRANPKDRWPSMEVVVAALVRGRGRKSRIVRAVVIAGVLAAAVASVGIVVAKRS
                     SHPATLTDKQRREASYTLMAWEDLRPGCSCPFSVCEKGACLSSCNATDFDYGQRAAIP
                     GVTVDGNQEALAGVSGDEQTLLYLSGRRCALDRVMVARRGGAAYVSTDITDRLDPKRV
                     KVYEGCCTLASDGRSMLISTPDRRGLVRVPLDAMAKLDGSEFADLLPDKAEGMTLGFP
                     VLSHDELTIYFTVYDRVASPTEEGPLDGVYSSTRASTSAPFPPGTRMPGVARQYAYVT
                     GESADGLTLFMASEFMTRVLTRRTRDGKYGAPFEATIPARVAGWRAVPSRDCSRLYTT
                     ETPGGCAAENIVVIPAIHKPPVVPYDPNAK"
     gene            complement(5174..7861)
                     /locus_tag="VMZ53_06560"
     CDS             complement(5174..7861)
                     /locus_tag="VMZ53_06560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28150.1"
                     /translation="MKDSSTATERVGRYQLLEPIGGGPNGMVSRAKVFGVAGFERQFA
                     VKRFLAELTAGPAAQALSQAARSYGSLEHPRIARMSEFGVAQGQTFTAVELVAGLDVF
                     RLMQESKLAAVAIPPGGALALVSQAARAVGYAHGRGLSHLGLSPTNVIVTADGDVKVT
                     DFGILAATMPSKPVDTPRLSQRIPYLAPEQLASEATSAATDVFVLGVLAYELVTGQRA
                     YRGDYPAAVAQAILAGPPAEPPLPRPIVRVLQRCLARSPFERFPDARALADALDAALR
                     VAPVPGTRKDIGAQIKTTLERYATLNEGNLSGMVALNLGAQQPEPSARPEARTEPSAI
                     RVVSPPSSPASTMRDMPKPTSTMPGLAPPPIPVPPGVTPPPTNAGLAQPGSSPPSTML
                     GVAASKKPTGIPSIKPPTSIPSITKPTPPAPPKVGALTQGWDAMPDATGGEVLEPSGL
                     IEVDDSGPVKIPHRPPSEDDEDGTMPFRDPNTMQGMGSPPNAPPSSPAVMSPSRLRPP
                     TPGQTFDSTTGEMSRAMLESLNRKATMSTAELAPQRPSMPEITIEPGTSPSLPPAPPG
                     PHDHEEDAPGDFAADASAVANDIAEQSPSATMPGHAAPPRRDSQPGVSQPSISSPPLA
                     PPAPSNPMVRPLVPEQKSKLPIALLGLLLVGGLGAGGYYLYTTQSAKQEPEVATGSGS
                     AKVVANTVPVDAAVAQPKQIDAAAIAVVATPDAAQTAPTPPDAATQVATAPPDAAAEK
                     PGPDTTNAVKPSGSSDQLQINSNPKGARVFIDGADQGTTPLKLPGSTDRHSLAVVLPG
                     KDLYLAEVDGHGVFDVELKDVEKGPKAFGGIKVLKCKKDRYYVYVDGRATGQMCPTER
                     INVPTGAHAVEIYDLVTESRRKFNIVVKDEGASERIRLE"
     gene            7973..8590
                     /locus_tag="VMZ53_06565"
     CDS             7973..8590
                     /locus_tag="VMZ53_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28151.1"
                     /translation="MARKEKEPGKGKDVGDDTDDAKPDAELERASAETQPIVVKRDDE
                     LPEITPPDPKGEPWPDATPQPMKPVDAPDLARARTATVPVTAVDLGRPPDMPRRTSPP
                     PLVLGTVEDPTHMPGPKEIPSGAPDDPAPAPGVVPPGDSRSLRRGDEFALIYRVGTCV
                     ITRVGRVGTRGMWRVVEYPTYAFASNSYAKECSRFVTDGFSDYRQ"
     gene            8598..9440
                     /locus_tag="VMZ53_06570"
     CDS             8598..9440
                     /locus_tag="VMZ53_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS28152.1"
                     /translation="MADEKSKSRKDLALRSSWLVRWAIEAPKRWLGRRSSIGQDVTGG
                     ADGISYLRHLVRGNRLRRRDAFAEVTPESPPVLIIHGFLGTRGSMYMLERRLVEDGFV
                     CVSFNLGTLNVRDIRRSAFLIHRKIERILAQTPSQKIDIIGHSMGGLIGLYYVKKLGG
                     HTRVRKLIMMGTPVRGTWAALAGVVTLGLWSTSSWQLLPRSRFLDELAQGPTPPGVDI
                     STIAAARDWVVPLSTTRMQGANAMTVPLGHSSLVVSEEVYRRVVNTLRPPHEHIHEEL
                     PETD"
     gene            9514..10446
                     /locus_tag="VMZ53_06575"
     CDS             9514..10446
                     /locus_tag="VMZ53_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUS28153.1"
                     /translation="MNDRPRFALRFISGKYQGGEFPLRMNREIIIGRSSDLDMVLVED
                     MVSRRHAKISSTDAEVYIQDMGSTNGTFVNGEKINGRALLHEGDRILVGTSIIKVVAV
                     EGQVAQQSEAEARRRLEAGAQQRQATQGRPMSGVIEEIPLPDLLQLLSTSRKSGVLAV
                     NNGVSIGKIFLRKGQIYFSTINDDFAVSPQKAIYRMLTWETGTFELEPGGEMQVMNEV
                     QESTEGLLMEGVRQLDEFRNLQKQLPPLGSPLAVPTPLAGKLRDLSPGELDTFQLVLD
                     HGQLQKVLDNFPGTDLDAAQNIIQLMKREFVVVP"
     gene            10507..10929
                     /locus_tag="VMZ53_06580"
     CDS             10507..10929
                     /locus_tag="VMZ53_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28154.1"
                     /translation="MPPSTVRIETIAPDALRSPYRGIATARETLRLTVSIAPRGMLPC
                     EIMIDVDDTSVQVCDHASGQCFALGAIEQLFVVERQPPAWRFLTWFGVFARRRGRPDR
                     LLLETPDGKVARFVERTIEERLGLRDEPVRGELRLAME"
     gene            10947..11756
                     /locus_tag="VMZ53_06585"
     CDS             10947..11756
                     /locus_tag="VMZ53_06585"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUS28155.1"
                     /translation="MIREAASIILLRRIAVGAGFEVFLVRRHRGSSFMASAYVFPGGA
                     AEEGEDARTAAARELFEEAGVLLARQSEDRDSETLELMSQAAMRKDVLEGAKARSVLA
                     AAGLQFSTENLVPWSHWITPSIEPKRFSARFFICELPTGQEPQFDATETVDQAWVTPE
                     AALARASELQLPPPQIRTFWELAQLESISKVIAAGRARAEQPHPIMPRLMPGTAPALL
                     LPWDPEYLTKGTGESTPLTYQPAWATGPSRFILDGKSWKHVAAPGSTSAGS"
     gene            11711..13651
                     /locus_tag="VMZ53_06590"
     CDS             11711..13651
                     /locus_tag="VMZ53_06590"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HUS28156.1"
                     /translation="MEARRRTWFDERGLVVASPGGDRRLPFYAGAMHYWRVDPKRWAA
                     CLKSIHLLGLTCVETYVPWRVHEPEAGALDWSGERDLGRFLDAARTAGLSVILRPGPH
                     INAELTSFGIPDWVLADPDCQARTAHGTPAWLPAPPRAFPIPSYASTKFRARVKAWYA
                     EVAKIAAPYLAPDGPVVAIGVDNEAQHFFRLGAFDLDYHPDAIAWWHEASGLDGEPPR
                     EWSEEQAARCILWLKHKDEYLARALGDFSRSLDEEGFAGVARFHNLPPGHHGLNDIRQ
                     LQHAIAGPVGIDAYTPRRLFPELRQRAFSLVGNATPIPLAPEVGIGFFPWFPPLDETT
                     DDPTRERDHLLTLLACGVRGFNLFMAVERDRYYGAAIAADGKLEPHAQWIKPIVAALA
                     EVDWPTLRRRPLIAVVDSKADARFGVATNVLDPVTATLADALDLGPGGFAELGTDAAA
                     IAARRWHHAITRALELAQVPYAIVDEATPEDELATYRAIVAPTPGTRIDRGLWQRLRA
                     LAEHKRAIVVVGPSTPTHDELDQPLADAAPKRIGKLKAGSLEDVAGLADDLAGLAGET
                     SDAWNVERPDTVRTMAYSTPDGATRVVFVVSDADRATTAVINAGDGAKALRDIYGERL
                     RVDGGRVTVAMPARGVRMLIVE"
     gene            13653..14057
                     /locus_tag="VMZ53_06595"
     CDS             13653..14057
                     /locus_tag="VMZ53_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28157.1"
                     /translation="MGGAKKRETVHVPLPACAREWSARERTIIDLHFYYLTNVWSLND
                     WKVYNRYRKHLPAESRFEVDQLVDLMMKDLPREMRAKLDAIFAVVWDAEDWLAVLASE
                     ELAHDLDVDDRNAWRQKVADVMLKLARDGALH"
     gene            complement(14054..14548)
                     /locus_tag="VMZ53_06600"
     CDS             complement(14054..14548)
                     /locus_tag="VMZ53_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28158.1"
                     /translation="MKAAKWICLAGAVLAVVGFFLPFVTVRDEKGSDSITPYQMVFAG
                     QDTQAQLTAGGTTPDQITADLREVAPIMVGFWVPAILLAIFATIAIVRGRMSNGLAIL
                     AMLAALANIGAWFVFHEAMLEMNKEEPTLRIALGIGTHALLAGGLLGFIAAAAAMFRP
                     ERAI"
     gene            complement(14561..15007)
                     /locus_tag="VMZ53_06605"
     CDS             complement(14561..15007)
                     /locus_tag="VMZ53_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS28159.1"
                     /translation="MGSILLVESDPETRDRLGLVMREAGHEVLVAITPREAFLRVSEG
                     GIDAVVVDSYDPRVGVIDLAKNFDALPDAPPVVLVSASPHAPEISARIGAAAFLPKPL
                     DPGELVNAISRLVGEVRPVRQIDASMEDFAAGDEEPTGPVRISSLR"
     gene            complement(15109..18168)
                     /locus_tag="VMZ53_06610"
     CDS             complement(15109..18168)
                     /locus_tag="VMZ53_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28160.1"
                     /translation="MQDQVQKSLKLVGRVLGDKFKLTACIGIGGSGAVYKADQMALGR
                     TVAVKILNEELCADPRMITRFRGEAMSASRLNHPNCVSIIDYGQSPDGLLYLAMEYVK
                     GPTLTQLLVNESPLSIERVIDLVAQALAGIEEAHLAGVVHADLKADNIIVDQRRANID
                     AVKIVDFGIARLVTGVRETEDRSISGTPEYMAPEVISGAPPSFASDVYAVGIILFELL
                     AQQTPFFAGSTTEILTNHLKAQPSPLTSRRDGVPRELDAVIAKALAKHPSDRYSSAAE
                     MREALLGIRARMHRLPSTDKCITCGASVNPSFKFCPECGAPRARVSKTFEVPATPTLP
                     DVLPLSFTGRSPELQGLLAHMRREPGSNGGLLVVGHEGAGRSTMLRNAYSRLGDEGRV
                     MYQVVPDPSGLAAPFYPIRSLLAAILQLPPVSSELELRETVLSIGLNERDIPGIAQVF
                     GHPTTLLELEPPVRRREMVWSSLRALERAAVHNPVTLICEDIDRFDSPSLEILRRATE
                     SNDLALPPIVMTATHAFAQQWPQSIKRLEIGALEAADLKSIVDDLTKAGVSGLPPVQQ
                     LFETTRAYPGHVEHLVRYLLEGGKAEDTKVSLPDLIAARLSMLPQSTRDVLQAAAVLG
                     IEPHLDFLRSMMPTEQLEPALQDAEEHGFITKDPSGMLVFASRLVREIVYDATPADVR
                     RSLHASAAAAVQAMSNDIGLLGHHHDLAGHARDAIELLRTAGDAAAEQLDEAGAGQFF
                     YRALVAVRQAVQSGDIEDGAEHQFVALSVRLADVLRTRGDTALARGILAEARDWSSAP
                     LLVSMIDRAGAAIALSEGDIDGAIAGLRRGVGRAIGSGDMNLVCELYLDLAMALQRAG
                     DPDTANRELVECVDLATLGEGFTATDGPESFWRVLRAQAQLVDAAGDSYRALRLAEAA
                     LFHAQRVRSRLGAARVQQLLASLCDKAGLGGKADRYRQAAITEMRSLGDRRATAELLL
                     TDTPSRGAAVVMPHRLQDAMQLTKEIGWSEGHERAKRKSSPPESSSQ"
     gene            complement(18247..19458)
                     /locus_tag="VMZ53_06615"
     CDS             complement(18247..19458)
                     /locus_tag="VMZ53_06615"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HUS28161.1"
                     /translation="MTRPAYLVDGIRSPFGRYGGGLGGVRADDLAAAVIRALVEKTKI
                     PTERIDDVILGCANQAGEDNRNVARMALLLAGLPVSVPGVTVNRLCGSGMQAIADAAR
                     LVMTGEADLVIAGGVEQMSRAPLVMGKPTEAFQRGNQTIYDTTLGWRFINPKMQEAHG
                     TLAMGETAEKVAERCNVSREAQDRFAITSQQRAGKAIASGRMALEITPIVTGTDKKTG
                     AKIEVTADEHPRPDTTIEQLQKLKPAFAKTGTVTAGNASGLNDGAAAVLVASEKAMKE
                     FGLTPKARYVAGAAAGVEVSYMGLGPIPASKKALEKAGVTPQRIDLAELNEAFAAQAI
                     PCIDQIGLDPEKVNVNGGAIAWGHPLGASGARMVLTLMHELSMRSNQFALASMCIGVG
                     QGIAVVIERTS"
     gene            19517..19921
                     /locus_tag="VMZ53_06620"
     CDS             19517..19921
                     /locus_tag="VMZ53_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28162.1"
                     /translation="MIHDFTTLAEHDVSWLAIKQPAVMRLLEAELATPDGDAFAAGLE
                     LACRVLGGRTPLGDLRLDHNSLAAGMTAVRNGRCDRAMVRSLRDQLEELPCVLMREEQ
                     DAVCTVIAAIIWTVLDASVRDLDDEVLNTLVA"
     gene            complement(19918..20559)
                     /locus_tag="VMZ53_06625"
     CDS             complement(19918..20559)
                     /locus_tag="VMZ53_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HUS28163.1"
                     /translation="MTLIVHRDERCVVVDKPSGLSTHRGWDDTDDALLQRVRDEVNAY
                     VYPVNRLDRGASGLVLFALDKEAAAAFASAWPTADKRYLAITRGHPPEHLVIDHAIPK
                     APGADRVPAVTEIWRREVFGRYALVEARPQTGRLHQIRRHLKHIACPLIGDVRYGKGE
                     HNRIWRERFALHRLALHCTSLTVAHPSGTTVVASAALPADMAAALDAARTSFS"
     gene            complement(20556..22730)
                     /locus_tag="VMZ53_06630"
     CDS             complement(20556..22730)
                     /locus_tag="VMZ53_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HUS28164.1"
                     /translation="MRRLMPLLALAGACRSNPSPLDPFVSHEMPTTSITTTSTTWPAL
                     DEKLLADAAETYNFKLGRPAPVAVTPDGAVLFRRTPARSFAADLFELDTKTGQVKTLL
                     SATAGDENLSDAEKARRERSRTATRGIVDVDVSADGRWVMAPLAGQFLLLDRTTGAKR
                     TLDPKGDAFDAHLSPDGTHIAFVRDGSMWILDVAGGAARQLTKHAEGIEHGSADFAAQ
                     EELDRYRGWWWSPDSKQIVYQLTDTREMDTIYVADARHPEKAPVPFKYPRAGRKNAKV
                     SLARVGIDGKDLGALPWDNVAFEYLARVSWPANGKLTISVLNREQTDLKLIAFDDAGK
                     ATELLSEHDDAWIVLPGQRSGFGHAPGTPLWKPDGSFLWMSEKSGEWAIEGLAIPKFR
                     NLIGLDGNEPIVEIEGSDPLRSKIKRGAWESKDDGVTDAHFENGILVVTCLLTNGGAR
                     AYAVTKDGKEIELPSVAETPSLVPTTKLESVTLEGRTHYTSIMRPRSFDTAQKYPVIL
                     KVYGGPNAQYVDASRDSYVMDQWYADAGFIVVRSDNRGTPNRGHDWERAILKDLITVP
                     LADQIGALQALGAKHPEMDMARVGVTGWSFGGYFSAHALLQRPDVFKAAVVGAPVSDW
                     KLYDTAYTERYMREPRNNEAGYASASVLTYAAKLSRPMLIVHGITDDNVHFAHTLALI
                     EALYIAGKRAEVITLSSTHMLPDPKLSLAREQQQIDFFRQHL"
     gene            22789..23169
                     /locus_tag="VMZ53_06635"
     CDS             22789..23169
                     /locus_tag="VMZ53_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28165.1"
                     /translation="MRKERRRRRAKTITLALSYQLDDCCKEGGIAGMVVADGDGLPLA
                     AAGDVYACDEVAARMCHVGSRIKEFNGTLLNGASRWDVQMTKVQVDGSDLLVCAVGGT
                     AEARKKQIARGAAGALRILNARAA"
     gene            23196..23669
                     /locus_tag="VMZ53_06640"
     CDS             23196..23669
                     /locus_tag="VMZ53_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28166.1"
                     /translation="MRTLLLVAVLAACSSSHKGNDVPDDEARKLLLDRNWLDRMPETE
                     RDRLHVYRFVPSMGGGVFQDRTLYKGEFELFRFETAADEIRFDLPETHQKVVSKFRIE
                     KVDGPKPFDLRLIIAADPRGPQVYYGMRSETDRDGAGLERRLHGSRTETTATSQP"
     gene            complement(23573..26173)
                     /locus_tag="VMZ53_06645"
     CDS             complement(23573..26173)
                     /locus_tag="VMZ53_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28167.1"
                     /translation="MSRCPLVATPSLVVKARSLFATAMIGCAVLGATAEARPWRAERV
                     ASAGAVDDAAIAERDARRYVAAHLPSGATLADFTLIANQLDGDLRTVSFQQTWRGLRV
                     VDATVAVVFGRGRDGDRLFAVKSRAIDDIAAPIVARRRAPRGELVVLQRGGTSTIAEV
                     TEETSSRTPERWRVYRDADDRIVGREKLVMDATATLKFNAGVRYARGARQDYDAVRDS
                     ITINGTTGATTATGIVTWAGTSPATVTPSTTGLYVRVNNSAGAAATTTFTGVSPGSTI
                     VWDLSSDELGDAQLSTFVYANRAKERARIVNPAASAWLDTTMDFYVNAVGECNAYSTG
                     NEVYLYRGTDKCENTGRIADIVFHEFGHALHKHSIISGVGAFESALSEGLSDFFAANI
                     TEDPTIGRGIYLADESPLRNIDPVGLERVYPVDLDFDDHVSGLIIAGALWDLRKGLIS
                     KLGYTAGVAQAEAIFTGVMQRADDIMTTFDAALVADDDNADLADGTPNYCAIENAFGR
                     HGLVPGYQTTTVSQPLVSGLHVALMTSTPSGTTCAAPAVTHAYVTWKAGDGVASVFEL
                     THDGDAWSGDIPEQPTGTVVSYSVDVQFDDGTSQVFPNNPADPLYQLMAGVPSQIWCE
                     RMDADPMWTQSSSEGIEWQWAAPIQNPSSGDPSFAKTGGSVLGTDITGDGRYRDSIAT
                     SILTPVVDVSRYQHVHLQYWRWLTVEDARYDQATIFANDEPVWHNVFSPTGTLDHVDR
                     EWRFHDIDVTPYVREGTMQITWGLTSDFNKELGGWTLDDACLVGLDHIGSCGDSIVEE
                     GEQCDDGGLDDDDGCNHLCILEPSAGGGACSAGRDADYGWLVAVVSVLLPWRRRSRPA
                     PSRSVSDRMP"
     gene            complement(26243..26593)
                     /locus_tag="VMZ53_06650"
     CDS             complement(26243..26593)
                     /locus_tag="VMZ53_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28168.1"
                     /translation="MRSALLLFGVGIALGACKPGGVTKEQELCAKAAAMFDKCEELDL
                     GSGSGAALSRELTLDRWRGLCRAVLTGETAQLMPNARELYQAMDDETKAGLKVQAECQ
                     AKAKDCADYKKCDE"
     gene            complement(26777..27925)
                     /gene="metK"
                     /locus_tag="VMZ53_06655"
                     /pseudo
     CDS             complement(26777..27925)
                     /gene="metK"
                     /locus_tag="VMZ53_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829366.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
     gene            27990..28700
                     /locus_tag="VMZ53_06660"
     CDS             27990..28700
                     /locus_tag="VMZ53_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28169.1"
                     /translation="MRAWVFACLVTLIACGGKPPIPKRGVVEGDLGAWKFRRFQPVLD
                     VEVWVENNKAEAYTASYVADDAEKKGHVEDKDVVNVFVTRYEKDDGVLRETVKLVRRL
                     AAEGGYQVDEHKVGGARALTINGHGESWVMWASTKHVVKVGGRGRETVPDSVVSSYAK
                     RYPSKIPGGALEGPLPAGPDNQGPGPEGSPEYDPNNPHADASKIDTSKVKLPDTKEKD
                     VPSDKPDDKKPDPKKKKK"
     gene            28710..29975
                     /gene="hisD"
                     /locus_tag="VMZ53_06665"
     CDS             28710..29975
                     /gene="hisD"
                     /locus_tag="VMZ53_06665"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HUS28170.1"
                     /translation="MLLRQIAIGSPEAAALLDRSTRFEDGVDDTVATILRDVRTRGDE
                     AVAEYTRRFDQREPPYEVTRAKWDELAAKVSPRVKAALELAASRIRAFHERQAEPDVD
                     VTLDGVRLELRVTPLARVGLYVPGGTARYPSSVLMTAIPAKVAGVREVIMVTPGASAE
                     AMLAARLAGVDRVFELGGAQAVGALAYGTASVPRVDKIVGPGNAWVASAKRQVYGEVD
                     IDSIAGPSEVLIIADNDADAAWVAADLLAQAEHDREARAILVTTSTSLASAVAAEVEA
                     QLATLPRKAIAEHAISHHGAAVIAGSIDDAVAFANRYAPEHLELDVADARAVAARCTT
                     SGAIFVGKWSSEAAGDYVAGGNHVLPTGGAARYASPLGVHDFRKRTSVIEYSREAALA
                     HADEIAALAAVEGLDAHGRSALRRKGGKP"
     gene            29972..31120
                     /gene="hisC"
                     /locus_tag="VMZ53_06670"
     CDS             29972..31120
                     /gene="hisC"
                     /locus_tag="VMZ53_06670"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HUS28171.1"
                     /translation="MSGDDRGDLGPLARRVPEALRIGVAYHVPQPPNLRAKLDANELP
                     YPLPAELRARLGAALAEVALERYPDPNARTLRALLADQLGVAGDQITFGNGSDECIAF
                     LIAAFSAPRASTQFGAARLHRERPAAVLYPAPSFVYYRIAAIAHGVEPIEVPLTPRFE
                     LDEGAIVRAVETHKPSVVFLALPNNPTGTLWRMDFAAELAARHRDLVVVSDEAYAAYS
                     GKSNLPALAAHPNLVVMRTLSKVGMAGMRVGFTIAAPAIAELLEKVRPPYNLSSLDQR
                     AATFLLTEGRAWCEARAAEVVTERQRLAENLAALRGVEVFPSEANLLLVRFAGDGKAS
                     RVWQGLAERGIVVRNFDRPGTPLAGCLRITVGTPDENKLLVEALHALA"
     gene            31143..31532
                     /locus_tag="VMZ53_06675"
     CDS             31143..31532
                     /locus_tag="VMZ53_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28172.1"
                     /translation="MSLCCSGCHTYGGSRTTAIVGGGTLAVGGGLWVASRTIDSPDTS
                     TNLKWAALGTASLGLLLAITGVIGMVALPTDEEAARDLARVLVSKAHQGNCTVVRDRA
                     HEVEELDTGVYDRLMLSDDAVKHCLAQ"
     gene            31517..32014
                     /locus_tag="VMZ53_06680"
     CDS             31517..32014
                     /locus_tag="VMZ53_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28173.1"
                     /translation="MSRAVALALALAVATSGCATYEGSRNTAVASGVSFAVGMGTWIG
                     GSQADSAPTAVAGLALGTASMMVVMASAVGMAILPKRVEIALRLAHELVVRAEAGDCE
                     TVTLRRAEVEELDALVYEVVLMEDPAIVECFGSSPSSSSPSSSPSAPAVVPVRLDPAS
                     TSTVR"
     gene            31909..33615
                     /locus_tag="VMZ53_06685"
     CDS             31909..33615
                     /locus_tag="VMZ53_06685"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUS28174.1"
                     /translation="MLRFVAIVVVSVVVAIGAGCGPGSPGPGIDVDRAMSEGAEIAFP
                     RHYNGEPGMRVQGFIMRTLEAMGVPYEYMPVGTVDVPRIEVMGNVYRERESLRVDDAN
                     LVVRFGPPGKALLVMAHYDSVPGSPGATDNAAAVAIVLELARVLKAEPPQRAVMLVFT
                     AAEEVGLVGAEALAAKLGDEIDFAIALDLIGGDGPLVVNGASMLVGRAELAWLRDGAD
                     RAGVELQFPLVHRVVSRWWPQAERSDHGPFTRRGVRAIHFYNRGNDGEWIDLAYHSER
                     DTWPRVHREQVAAVGRLLRALTADDSAHDAVPAYDGDGFVVPVVHWVIARWVLVAIEL
                     ALAIVTVLALVRQRRVRSPGAGLLIGTASFIVAVVLAIIAERLVLPYRGAWLLSPLRS
                     TLALALVLAGTFGLLTRVVARFTAWTGSLRYRAFASLTCLAIGLAFLAIGAAELAWIW
                     LVPALVIAIAPAAVGVVASLVPAILVLHPFQLREAAWNGFLPASLPLAAAIGLLGAPT
                     IAAVAWAWSSRRVRPGPLGTLVLGVGCGLAVSLGILLAGTAKTTCSSVEFGRFGLACE
                     RV"
     gene            33695..34249
                     /locus_tag="VMZ53_06690"
     CDS             33695..34249
                     /locus_tag="VMZ53_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28175.1"
                     /translation="MATAATAIFTACDVGSVVQNSTGTDGGTKMDGTGSGSGSGSGSA
                     ANCEEISATPPDGHHNPGQNCIAAGCHLTGQLGTNAPPFTYAGTLYTTAAGTAPVAGA
                     TIFVKMGATEKKVLSANNGNFWMGPAPAGINAPTNAMTATTKASGCALAPNHVPMVGA
                     LVQGGGNCNNCHRNGGTTTPIHLP"
     gene            complement(34250..35500)
                     /locus_tag="VMZ53_06695"
     CDS             complement(34250..35500)
                     /locus_tag="VMZ53_06695"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28176.1"
                     /translation="MRLGEMLVRDGRLTEAQLNQALQYQQRDGARLGTVLVEHGLVDL
                     EALTVYLGLELGIPIATGAMLERAKRAAVRLLQPTAAFKHKCVPLVVQDRQLIAAIED
                     PHDIATLESLTQITGYRVLPRVAPEVRIYYYVERYYGVARPARFMKFGDTPRGTESQG
                     DAGLPAPPLPGLPPVPAAPVAAPGPRPMLKSTKTSSLSPTSSFDESALELEAEDLLES
                     LDADDAAPAEAQTHLRPHRVTTPPAFVPTAVDPGRPGAISVEVALTELATLTDRNRIV
                     EVLLGYASLIFDVAVLFAVRDQMAFGWKAFGNIVGRGYVEHLLIPLDAPSVVQAATAA
                     EGGLISGPVTPSTVNNYLYKVLGCSEPRHATAGVVTIGKRIVNVLYGHGKELSPLQIE
                     DLRQVCTSAAEAYARLIAVQKKKK"
     gene            35579..37087
                     /locus_tag="VMZ53_06700"
     CDS             35579..37087
                     /locus_tag="VMZ53_06700"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HUS28177.1"
                     /translation="MRALACCALLALAGCGNKADKPAPAPLPTPTGNPPTGTPGEPAS
                     VAQPDFPEDTRSLELTRTIPVRLDPGDDGKRIGTIAIDTRVGWQRTAKAKGCSKAWVE
                     IRPRGWVCGEFLKASKLPPQGREVPQLERGEIVPGIYGRVTASAGAQGSVLYKLQKPE
                     DKKPKAKDTKKKGGGPVTSPSQLDDKPKEARMVEADPIVGTLTIRQYEELTVDGKVYW
                     KINPKTNEYVLKSLVNLMKPSDFKGTRLGDDTGWKLPVAFIWPRNNWTQAWTYAKPNA
                     ISYRQINARLPVAILETATNKAGKAIAYRIADPTPQAPLGEWVNVADVRVFAPVDPPP
                     LLTPNERWIDVDLDNQILVAFEGTTPVYATMVSTGAKDTPTLTGMYRIWLKASEADMK
                     NLKAEDPYSVATVPWTEFFYSEDDLALHTAYWHDAFGKTRSHGCVNLAPRDARWLYYW
                     SDPVVPPGWTATSGVVEAPGSVVRVRSAADPSPPWRGYAKKVAEARQNRNAE"
     gene            complement(37511..38173)
                     /locus_tag="VMZ53_06705"
     CDS             complement(37511..38173)
                     /locus_tag="VMZ53_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28178.1"
                     /translation="MSLLTDEPTIELDHPKWQRAEIAVFTRRVVADCMSHQCTIVDGN
                     KLKLDACCQYGCDVDLTERDAILARADLIRPVLAAEVQNLPWFDESAPERDPDTPSGT
                     VVRTAVHNEGCLFLTHDRRGCAIHRASIEQGWDFRGTKPTICRLFPLTYGEGAIFVSD
                     DYPDYSCAYHPDAPTLYQVARDAIGDIFGPELVHAMDAAEKQVLAMQPKKLAVVRDER
                     TA"
     gene            complement(38190..38843)
                     /locus_tag="VMZ53_06710"
     CDS             complement(38190..38843)
                     /locus_tag="VMZ53_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HUS28179.1"
                     /translation="MRLTVDFGSPSASAAPVKLLVSTTFVKTTMSLRSMANPGCPGHT
                     LKPMSRYPELAAKVDGFFARAMERHGDDMRCDTGCSHCCHVRLSVTRVEAEAIRDELS
                     SWPAERRAVLARNIAAALPDRCVALDADGRCLVYASRPIVCRSHGAPIRLTQKSLPIV
                     TSCRENFTARGPAAADADCILDQATLSALTLAVDRDAGGDGSRTDLDSWLATVLADC"
     gene            38740..39645
                     /locus_tag="VMZ53_06715"
     CDS             38740..39645
                     /locus_tag="VMZ53_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUS28180.1"
                     /translation="MDLNDIVVFTKVVETKSFTGAADALGLPKSTVSRKLAQLEERLG
                     VRLVQRTTRKLALTEIGEAYYERCSRIVADVAAAEQLVTDMQSTPRGRLRVTSTIDFS
                     TRFLGEIIADFLAQHPDINVELEATDRVVDLIDDGFDLAVRFGPMPESTLIAKRLVSL
                     HLILVASPEYLARRNMPKTVEELDEHDHVLFTPASRNQTWTLVHNNGGEQTYEFGRPA
                     RLASNNYGAVVDVARANGGIALISEFMITDELKTGRLVHVLPEWKTRPTDVHAVYPAR
                     QNVPPRLTLFLEHLSKSLNPPPWAR"
     gene            39645..40028
                     /locus_tag="VMZ53_06720"
     CDS             39645..40028
                     /locus_tag="VMZ53_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HUS28181.1"
                     /translation="MPLSHRPLVAMAALNGLAAVGAGAFAAHGLRSRLEPRALEIFET
                     GARYQMYHALAMIACGALMVAWPSVSRGARNAGWLFQAGIAIFCGSLYALALTDVKML
                     GAITPIGGVLFLLGWLWLTISALRS"
     gene            40056..42464
                     /locus_tag="VMZ53_06725"
     CDS             40056..42464
                     /locus_tag="VMZ53_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28182.1"
                     /translation="MPGDDKTASNGLSQASGSDETVVDSPSARSRPTPPPREVIGRFT
                     IDRELGVGGMGVVLLAHDPTLDRQVAIKLLRDERSATSNARVRLLREAQAMARLRHPN
                     VVTVYEAGVHEGQVYLVMEMLPGGTLRQRLSGEVATRDILRLYIEAGRGLAAAHRAGL
                     VHRDFKPANVLVDEADHAKVTDFGLAVQDDSTEREARPRTSEPDLDALAHPLTRTGDL
                     VGTPRYMAPEQHAGKDTDPRSDQFSFCVALYEVLYRQHPYRDESLEALRKSMRDEEAR
                     APKSTGRVRNAIMRGLSRDPANRFASMDELLAELAPRRSRAGWLAGGAAALLAIGAVA
                     FALSPGHASDPIAQTKRAALACNCELLDVQVPAGARVIGVRVVGAGGLGPLVVAAWQP
                     TGQLALARGSHVIEYLVGDARYDFAVMSRGEARTRTLALPPPEQRTGYVFVPGGEVTL
                     LGDTSLVELRPYLIESTATADLVSYADARKRAFAQHARLPFAAEWEWAARLGVFPRAG
                     WEWTGSAFAPYPLGPIARDDEAAPRDRSEVRGGSGDLAKRLAGLHTGRAGSRLARDVT
                     SSLVLEPVTVQLAEVPVAGDSTSLEVVVPPAERARLDRFLTTWRQLDPQPALIVEGDT
                     CNITGIVYANGASPTHVEVNANNTASRRATVRVHPDVSYTSASKHVTICTNPSIEILD
                     AICFTGDKLHPGSTRLLEAIAASLSASPTWKRVEVQAYADSDSATLAQRRAEIVRARL
                     IALGVASERLVARGYLNDGDIDDPEHGLVAEQRAAQPPAGCPAPRPGERIAFKSLDPA
                     DAAE"
     gene            complement(42466..43491)
                     /gene="rtcA"
                     /locus_tag="VMZ53_06730"
     CDS             complement(42466..43491)
                     /gene="rtcA"
                     /locus_tag="VMZ53_06730"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF003246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HUS28183.1"
                     /translation="MRLPVDIDGSRGEGGGQILRTSLALSVITGRKLRMKNIRAGRKK
                     PGLQRQHLACVEAAARLSSAQTNALDVGASELVFTPSPSEAWPTELHIDIGSAGSTTL
                     VVQTILIPALFAPHPVKAVITGGTHNPLAPPFEFLDRVFMPPLRAMGADVTLTLEKHG
                     FMPDGGGRIVMETKPSKLKRIELVEAGKIVARRATAICAKLPRHVGDRELAVAQERLA
                     DPTCELREFPNDGPHNVFMVEVELASGARELVTSHGRKGYPAEDVADDALDDLEDYLE
                     AGVPVGECLADQLLLPMAIAGGGKFRTLAPLSEHTLTNIDTIREFLDVPIRVEPIEVM
                     INDVVIG"
     gene            complement(43501..44715)
                     /locus_tag="VMZ53_06735"
     CDS             complement(43501..44715)
                     /locus_tag="VMZ53_06735"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HUS28184.1"
                     /translation="MSDYELIETSGVPIKAWTRGVGVEDSARRQLENVARLPIVFHHV
                     AVMPDVHFGIGATVGSVVPTENAIIPAAVGVDIGCGMIALETDLPAAQLPDSLKSLRT
                     AIEKAVPHGRTSNGGRGDRGAWSKLPTAANKAWRDLDSTFKEIVDKHPRIGGGNDVNH
                     LGTLGGGNHFIEVCLDERDKVWFMLHSGSRGVGNRIGSYFIDLAKRDMRRHINNLPDN
                     DLAYLREGTTHFADYVQAVEWAQKFAMTNRQLMMDAVVGVAKKELPPFKLGELAVNCH
                     HNYVAREHHFGKDVFVTRKGAVRARQDDLGIIPGSMGARSFIVRGKGNPESFHSCSHG
                     AGRTMSRGEAKRRFSVEDHIKATEGIECKKDASVIDETPMAYKPIDKVMAAQEELVEV
                     VHELRQVVCVKG"
     gene            44847..45134
                     /locus_tag="VMZ53_06740"
     CDS             44847..45134
                     /locus_tag="VMZ53_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28185.1"
                     /translation="MFKALLFTIALAACGGHKAKPAETAPPPAGHEQGSDTSLTAQQC
                     TDQGGEVVGDIGDGATQQPDYKCAKSGLPPVGHIAAEPGQPQAIEGAVCCK"
     gene            complement(45136..45489)
                     /locus_tag="VMZ53_06745"
     CDS             complement(45136..45489)
                     /locus_tag="VMZ53_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28186.1"
                     /translation="MKRFAFVLLLAACSSNGSKTSQTSSSTQTGSAAVEPVPGVEAGD
                     SGSAAVTAETARGQKCGENDSCPTGLECVKYYGIAGPKGPQFTSCETKCDGGKACPDG
                     LKCTTIADGPGAVCR"
     gene            complement(45508..46629)
                     /locus_tag="VMZ53_06750"
     CDS             complement(45508..46629)
                     /locus_tag="VMZ53_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS28187.1"
                     /translation="MRRVAGSLCLVVSACNFEHGELGGAGLPMDSGGDVDAPDAPWRP
                     GFLYRKPIAITPPSLTAALTSFPVAILTTADAQLGDNARTDGRDITFTAADGTTLLDH
                     ELVTFDGASGALEAWVRIPTLPNGTTTIFMYYGAGMQPLTAIATWPSQFKGIWHLDAG
                     IGAADSTPNGHGAFSSAPLTTPTVVDGIAGHARAFDGIDDSMTVTNPADGSLDFGTTS
                     FSFSLWVNVAVSKGAFDAPIYKGGASVGDPGYCVLLGTADWNMKTHDGTSYRDPAVGA
                     EALGAWVYLAGVMDRDAQQMLAYRDGVFTDMMSTTGFGSITNNKALVFGPTGTLNFQG
                     TLDEVRISAGALSPEWIATEYANLETPGFVALGSQQMKP"
     gene            complement(46630..>48122)
                     /locus_tag="VMZ53_06755"
     CDS             complement(46630..>48122)
                     /locus_tag="VMZ53_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS28188.1"
                     /translation="GQRINVLVLEKALTLELTQFEDSELYDKMTRARREASSRPLSLV
                     SQAFELGQSMVTLIGLGGLLAAFSPIALCVLIAAAIPPFVAELKFSGDAFRLSRWRTP
                     ETREQMYVEMVIAQDAYAKEVKLFGLGPRFLRRYREIYEKLYAGDRKITISRGIWALI
                     LGTIGQAAFYGMYLWIAFATIDNTISFGDMTMYVLVFKQAQSALSSALGDVGGMYEDN
                     LYMSNLYEFLDTPTVMPSGDATTGKIPGDGVRFEHVSFEYPGSHEHALTDVNLHIKPG
                     SKLAIVGENGSGKTTLIKLLTRLYEPTGGRITLDGRDLREWDRTTLHHRIGVIFQDFV
                     RYQLKVGENIGAGDDRAYDDRTRWEDAAERGLARPFIADMPDTYDTQLGRWFKQGREL
                     SLGQWQKIALARSFMRKDADILVLDEPTASMDADAEVKIFERFRELTADKIAIVISHR
                     FSTVRMADQIIVLDHGKITERGSHEELVKQGGRYATLFNLQAQGYR"
BASE COUNT         7969 a        15742 c        16123 g         8288 t
ORIGIN      
        1 agtacggtct cgacccgcgc cacccgggcg gcgatgcagc gccgagcggc gaagacgatc
       61 tcgactagct gctactcgac ggtgtggatg caggctccac cctcgaacca gtggaagttc
      121 gtgatgtggt tgttgctgca cgtcacgacg aagcccgcac cgaacgcgca gcttccgtcg
      181 aatccgtacg acaggaagat gccgccgttc cagtcacacg acagctgacc tgcgatgtcc
      241 gcgtgttgga tctggccggg aagcagcgcg ttgttgctgc tccatgacat cgacgagacg
      301 cgcgtgccgt cgcagtggat gtggtcgcca aacgtgaacg cgctgccagc gttccagcct
      361 tgcgatgccc accggttcgc gttgccccaa tcgcagtcga tgtagtcggt gctcgtgccg
      421 ccggtgactt gtggcggact gagcgccgcg agggactgcg tctggccgga tttgttttcg
      481 tagctcatgc ctgcgagctt tccgttggtg caggtcgccc acacgcccac ggacgccgga
      541 tcgccgtcgt agccccacga cagccagcgc gttccgatcc agtcgcagtc catcgagcag
      601 cgcgcgccgt cgtcctgtcc gttgacacag ttgtcgtcga cgccgttgaa gcaaagctcg
      661 gtcgcgttcg gatggacgtt gccgttgccg ttgttgcaat ccgtgttgtt gctgacgtag
      721 cccgcgaccg gcggtgacga acaggccgcc gtggccgtgc cggcgccgta gccatcaccg
      781 tcgccatcgg cgtaccacag cgtctgaacg ccttcgtcga tgccgttcac gcagttgtcg
      841 tcgacgaagt tgcagacctc ggccgcgttg ggatggatgt tcccgttgcc atcgttgcag
      901 tcagtcgcgt tcgcgatgta accaccgccg gccgcacagc gctgctgcga ggcaccggcg
      961 ttgccgtaac cgtctccgtc gttgtcgagg tagaacgtgc tcgtcacgcc ttcgtcagcg
     1021 ccgcccgcgc agttgtcgtc gacgccgttg cacacctcgg ctacgcccgg gtgaatcgcg
     1081 ccattgccgt cgttgcagtc ggtcgcgttc gcgacgtacc cgccgcccgc tgcgcactgc
     1141 tggttcgtcg ctgccgaggt gccgtaaccg tcgccgtcgc cgtcttggta gaacgtcatc
     1201 ttcacgccct cgtcgatgcc ggcggcgcag ttgtcgtcaa cgccgttgca cgcctccgcg
     1261 gcaccgggat ggatcgcgcc gttcgtgtcg tcgcagtcat cggcgctcgt gacgtatccc
     1321 gtcggtgcgc cgcacgtagt catgctcatc gacaggcttc cgtgaccgtc ggaatccatg
     1381 tcgcgataga acgtcgtcag cccggtctcg tctgtctgcc cgttgcagtt gttgtcctgc
     1441 agatcgcaga tctcggttgc gctcggcttg atcgccgcgt tcgcatcgtt gcagtccgtt
     1501 gcgccggcga cgggcatcgc ggtgacccag gtgccgccgc atgcaggagc cgcgggcatc
     1561 gtgcacgaca tcgtcgtgtc gctcgtcatt gcggcgacgc cgtcgccatc gcagtccgcg
     1621 taccacgtca ccgcgttgca cgtatccggt ggcggcgcgt ccggcatctt gtagtcgagg
     1681 tcaccgcacg cagcggccgc caacatcgca atcacaatcg tcctcatgca tgcccccaaa
     1741 cgagttgggg gtaaggaggg ccctcccgga ttttgtcgcg ccgaaagtcg taacccagcg
     1801 gaacttcagc cgagtaaccg tgacaacgac acgtcgacgc gactctgtac gaggcgcacg
     1861 accgagtcga cctcctcgac gggaatcgcc aggcgcgctg ccagctcgat gcgcatcagc
     1921 tctccgagct cgtcgcggac ggccgcaaca cggcgcgcgg cggttgcgcg atggatgccg
     1981 tagagcttcg cgatgcgatc gatgctccag ccatcgacga ggctgtagcg gagaagtgcg
     2041 cgcgagtctg cagcaagctt cgacacggcg atgcgcatcg ctgcgtcgac atcaggccga
     2101 tacgcttcgc gcaagtagcc gacttcggga tccgagagcg gcgacagctt gtcgaagaaa
     2161 gattcctcct cgatgccgac ttcgcgcttg ccgccatcga tcacacgcac gagctcgcgt
     2221 gtcgtgatca cgcgcaggta gctgcgtaga tcgccgcggc ccgagtactc gctcagtgcg
     2281 gcgttgcgtc cgggctcgct gacgaacagc gtccggcgta agtagctgcg gacttcgtcg
     2341 gcttgatcgt cgcgggcacg catgcgttgc gcgcaagcag cgacttcgtc gaggaattcg
     2401 ctctcgaagc ggcggatcgc tgcttcgtcg ccgcgtgcgc atgcgatcgc tagatgcacg
     2461 tgatcggcgc gcacgcgtgc gagaagctcc ggcgttgctg cggcgccgag gcggcgtgcg
     2521 agctcgctgg cgtactccgt ctcggtgacc gcgatctcgg gaaatgccgc ggtcgcagcc
     2581 gcgcgcagct gcgccaatgt gtcggcgatt ccgacattgc cttcgaagcg aggacgcgcg
     2641 ctgacatcga gtgcgctcgc gaaggcctcc gacatcgccg acatcttgtg cacgagatag
     2701 taacgctgga cgtgtactct cgcagtcgtg gagtgcgcga acgcggacac gttggcgggg
     2761 ctcgtcgccg gggcgctgcc cgaggccgag cgcgctgcga tcgcgacgca tgcggcatcg
     2821 tgcaagcggt gtcacgcgct catcgacgag ctgttcgaga gcagcagcgc gggcggacct
     2881 gtgacgccgg cagggcccga cgatacgacg cagggcgacg agccagatgg cagccggccg
     2941 tcgaggattt cgctgcgacc tggcgcgcac gtcgggcgtt atgtgatcga agagcggatc
     3001 ggtgcgggcg ggatgggcgt cgtgttcgcc gcgaccgaca gcgagctgca tcggcgcgtc
     3061 gcgatcaagc tgctgcggca aggcggtcag gggcacgcgc gaatgagcac ggccggtcgc
     3121 gagcggctga tgcgtgaagc gcggctgctt gcgtcgctgt cgcatccgaa cgtgctcacc
     3181 gtgttcgatg tcggcacgca cgagaccgac gtgttcatcg cgatggagct cgtcgacggc
     3241 ggcagtctct cgagctggtt gcgccgcgcg ccgcgcacga ccgacgagat tattgatcgc
     3301 atgatcgaag cgggccgcgg tcttggtgct gcgcacgcgg gcggcgtcgt gcatcgcgac
     3361 gtgaagcccg acaacattct cgtcggtctc gatggccgcg cacgcgtgac ggacttcggt
     3421 ctcgcgcgca tcgatcagga gctcgcgccc gatatcgacg cgatgagggc tgctgcgctg
     3481 tcgcctgatc tcacgaagac cggcgcgctc atgggaacgc ccgtgtacat ggccccggag
     3541 cagtggagga agggcgctgt cgatccgcgc acggatcaat ggtcgttctg cgcgacgctc
     3601 tacgagctcg tcgccggcgt gcgaccgttc accgtcgacg atcctgacaa gcgcgcggcc
     3661 gagatcgaag aggggcgcat cgcagcgccg gcggatggcc gcacggtccc gagctggttg
     3721 cgcaagatcg tcgagcgcgg ccttcgcgcg aatccgaaag atcgttggcc atcgatggag
     3781 gttgtcgtcg cggcgctcgt gcgtggccgc gggcgcaaga gccggatcgt gcgtgcggtc
     3841 gtgatcgccg gtgttctcgc ggctgccgtc gcctcggtcg gcatcgtcgt agcgaagcgc
     3901 agctcgcatc ccgccacact gaccgacaag cagcggcgcg aggcgtcgta cacgctcatg
     3961 gcgtgggaag acttgcggcc cggttgcagc tgccccttct cggtgtgcga gaagggcgcg
     4021 tgcctgtcgt catgcaacgc gaccgacttc gactacggcc aacgcgcagc gattcctggc
     4081 gtgaccgtcg acggcaacca ggaggcgctc gcgggcgtct cgggcgacga gcagacgttg
     4141 ctctatctct ccgggcgacg ctgcgcactc gatcgcgtga tggtcgcgag gcgcggcggt
     4201 gcggcgtacg tatcgacgga catcaccgat cgtctcgatc cgaaacgggt caaggtctac
     4261 gaaggatgtt gcacgctcgc gtcagatggc cggagcatgc tcatctccac gcccgatcgc
     4321 cgtggtctcg tccgcgttcc gctcgacgcg atggcgaagc tcgacggctc cgagtttgcg
     4381 gatctgttgc ccgacaaagc agagggcatg acgctcgggt ttccggtgct gtcgcacgac
     4441 gaactgacga tctatttcac ggtgtacgat cgcgtcgcga gcccgacgga ggaaggtccg
     4501 ctcgacgggg tgtactcgag cacccgcgcg tcgacgtcgg cgccgtttcc gccaggtacg
     4561 cgaatgcccg gcgttgcgcg tcagtacgcg tatgtgaccg gggagtccgc cgacgggctc
     4621 acgctgttca tggcgtcgga gttcatgact cgcgtgctca cgcggcgcac ccgtgatggc
     4681 aagtacggcg ctccgttcga agcgacgata cctgcgcgcg ttgccggctg gcgcgctgtg
     4741 ccttcgcgcg actgctcgcg gctctacacg acggagacac cgggcggttg tgcggccgag
     4801 aacatcgtgg tcattcccgc gatccacaaa ccgcccgtcg ttccgtacga tccgaacgcg
     4861 aaatagcact cgcgatcgcg atcgtcagcg ccgcgccggc ccacagcagc gggacgagat
     4921 gaaccgcgac gaccgcagcc gcgtcgagcg ctgggaacag cgggtccggc ggaggcggtg
     4981 cggaccgtac ccgcagccac gagcttgcga tcgcgatgat tgccgcactg ctgccgagga
     5041 cgccgagcca gggcacgcgc gcacaaacga cccaccacgc gaccgcgatt ccgacgacgg
     5101 caagcgcgac gaccacggcg aactatacga agccgcgagc gtcacgctgc ggcaactcgc
     5161 ggcagtcctc ccgctactcg aggcggatac gctcggacgc gccttcgtct ttgacgacga
     5221 tgttgaactt gcggcgcgac tccgtcacga ggtcgtagat ctcgacggca tgcgcaccgg
     5281 ttggcacgtt gatgcgctcg gtcgggcaca tctgacctgt cgcgcgaccg tcgacgtata
     5341 cgtagtagcg gtccttcttg cacttgagga ccttgatgcc gccgaacgcc ttcgggcctt
     5401 tctcgacatc tttgagctcg acgtcgaaca cgccgtgacc gtcgacctcc gcgagataga
     5461 gatccttgcc gggcaacacg accgccagcg aatgacgatc ggtgctgccg gggagcttca
     5521 atggcgtcgt tccttgatcg gcgccgtcga tgaaaacccg agcgcccttc ggattcgagt
     5581 tgatctggag ttggtccgac gagccacttg gcttcacggc gttcgtcgta tcgggaccgg
     5641 gcttctcggc ggcggcgtcg ggcggcgccg tcgcgacttg cgtggcagca tcgggcggcg
     5701 tcggtgcggt ttgcgcagcg tcaggcgtcg cgaccaccgc gatggcagcg gcatcgattt
     5761 gcttcggttg cgccacggct gcatcgacgg gcaccgtgtt cgcgacgacc tttgcggagc
     5821 ccgagcccgt tgcgacctcg ggttcctgct tcgcagactg cgtcgtgtac aggtagtagc
     5881 cgcccgcgcc gagacctccg acgagcagca aaccgagcag cgcgatcggc agcttgctct
     5941 tctgctctgg cacgagtggc cgaaccatcg ggttcgacgg cgcaggaggc gcaagcggtg
     6001 gcgagctgat cgacggctga ctcacgccgg gctggctgtc gcgacgaggc ggtgcggcat
     6061 gaccgggcat cgtcgccgac ggagattgct cggcaatgtc gttcgcgacg gcgctcgcat
     6121 cggccgcaaa atcgcccggc gcatcttctt cgtgatcgtg cgggccggga ggcgccggcg
     6181 ggaggctcgg cgacgtgccc ggctcgatcg tgatctcggg catcgacgga cgctgtggcg
     6241 ccagctccgc cgtcgacatc gtcgccttgc gattgagcga ctcgagcatc gccctcgaca
     6301 tctcgcctgt cgtcgaatcg aatgtctggc ccggagtcgg tggccgcagc cgcgatggcg
     6361 acatgacggc gggcgacgag ggcggcgcat tcggcggcga gcccatgccc tgcatcgtgt
     6421 tcggatcgcg aaacggcatc gtgccgtcct cgtcgtcctc ggaaggcggg cgatgcggaa
     6481 tcttcacggg ccccgaatcg tctacctcga tgaggccgct cggctcgagc acttcgccgc
     6541 cggtcgcgtc cggcattgcg tcccagccct gcgtcagcgc gcccactttt ggaggagcgg
     6601 gaggcgtggg cttcgtgatc gagggaatcg acgtcggcgg cttgatcgac ggaatgcccg
     6661 tcggcttctt cgaagcggca acaccgagca tcgtgctcgg cggcgacgat cctggctgcg
     6721 cgagacctgc gttcgtcggc ggcggcgtga cgccaggcgg gaccggaatg ggcggcggcg
     6781 cgagaccggg catcgtgctc gtcggtttcg gcatgtcgcg catcgtcgat gcggggctcg
     6841 atggcggcga cacgacgcga atcgctgaag gctcggtgcg cgcttccggt cgcgcggacg
     6901 gttccggctg ttgcgcgccg agattgagcg cgaccatccc cgagagattt ccttcattga
     6961 gcgtcgcgta gcgttcgagc gtcgtcttga tctgcgcgcc gatatccttg cgcgtgcccg
     7021 gaactggagc gacgcgaagt gcggcgtcga gtgcatcggc gagcgcgcgt gcgtcgggga
     7081 atcgctcgaa cggcgaacgc gcgagacagc gctgcaacac gcgaacgatc ggacgcggca
     7141 gcggcggctc ggccggcggc ccagcgagga tcgcttgcgc gacggcagcg ggataatcgc
     7201 cgcggtacgc gcgttggccg gtgacgagct cgtatgcgag cacgccgagg acgaacacat
     7261 ccgttgccgc cgacgtcgct tcgctcgcga gctgctcggg cgcgagatac ggaatgcgct
     7321 gcgagaggcg cggcgtgtcg acgggcttac tcggcatcgt cgccgcgagg attccgaagt
     7381 cagtgacctt cacatcaccg tcggcggtaa cgatcacgtt cgtcggcgag aggccgagat
     7441 gcgagaggcc gcgaccgtgc gcgtagccca ccgcgcgtgc agcttgcgag accagcgcga
     7501 gcgctccgcc cggcggaatc gcgacggccg cgagcttgct ctcttgcatc agccggaaga
     7561 catcgagacc ggcgacgagc tcgaccgcgg tgaacgtctg gccttgtgcg acgccgaact
     7621 cggacatgcg cgcgatgcgt gggtgctcga ggcttccgta tgaacgcgct gcctgcgaga
     7681 gtgcctgcgc agcaggaccc gcggtcagct cggcgaggaa acgcttcacg gcgaactgcc
     7741 gctcgaagcc ggcaacgcca aagaccttgg cgcgcgacac catcccgttg ggtccgccac
     7801 cgatcggctc caacagctgg tagcgcccga cgcgctcggt cgcggtggac gagtccttca
     7861 cccggaccca actttacccc agcagcaccc gatgacgcga tgcgttggca accggatcaa
     7921 tcgcgggcac ctgaacttgc tctgcgcggc gaacgctggc accacggaaa ggatggcgcg
     7981 gaaggagaaa gagccgggca agggcaagga cgtcggcgac gacaccgacg acgcaaagcc
     8041 cgacgccgag ctcgagcgcg cgtccgccga gacgcagccg atcgtggtga agcgggatga
     8101 tgagcttccc gaaatcacgc cgcccgaccc caaaggtgag ccgtggccgg acgcgacgcc
     8161 gcagccgatg aagccggtcg acgcgcccga ccttgctcgc gctcgcaccg ccacggtgcc
     8221 ggtcactgcc gtcgacttgg gtcgcccgcc ggatatgccg cgcaggacct ctccgccgcc
     8281 cctcgtcctc ggcaccgtcg aggatccgac ccacatgccg ggaccgaagg agattccatc
     8341 cggcgcaccg gacgatccgg ctccggcgcc aggtgtcgtc ccgcccggag attcgcgctc
     8401 gctccgtcga ggagacgagt tcgccctgat ctatcgcgtc ggtacgtgcg tcatcacccg
     8461 cgtcggtcgg gtcggaacgc gggggatgtg gcgggtggtc gaatacccta cctacgcgtt
     8521 cgccagtaac tcctacgcga aggagtgcag ccgattcgtc accgacgggt tctcagacta
     8581 tcgtcaatag aagattgatg gccgacgaga agtcgaagtc gagaaaggat ctggcgctgc
     8641 ggtcgtcgtg gctcgtgcgc tgggcgatcg aagccccgaa gcgctggctc ggacgacgca
     8701 gctcgatcgg ccaagatgta acgggaggcg ccgacggcat ctcctatcta cggcacctcg
     8761 tccgtggcaa tcggcttcgt cgccgcgatg cattcgccga ggtcacgccc gagtcaccgc
     8821 cggtcctcat catccatggc ttcctcggaa cacgcggctc gatgtacatg ctcgagcgtc
     8881 ggctcgtcga ggatggcttc gtctgcgtgt cgttcaacct cggcacgctc aacgtccgcg
     8941 acatccggcg gtccgcattt ctgattcacc gcaagatcga gcggatcctc gcgcagacgc
     9001 cgtcgcagaa gatcgacatc attggccact cgatgggcgg cctgatcggc ctctattacg
     9061 tgaagaagct cggcggccac acgcgggttc gcaagctcat catgatgggg acacccgttc
     9121 gggggacctg ggcggccctg gcgggggtcg tgacgctggg cctgtggtcg acctcctcgt
     9181 ggcagctctt gccacgcagc cggttcctcg acgagctggc gcaggggcct acccctccgg
     9241 gggtcgatat ctcgacgatc gcggccgccc gcgattgggt ggtccccctc tccacgacgc
     9301 gaatgcaggg tgcgaacgcg atgacggtgc ctctggggca ctcctcgctc gtagtttccg
     9361 aggaagtcta ccggcgcgtc gtcaatacgt tgaggccgcc ccacgagcac attcacgaag
     9421 agctgcccga gacggactga aatcaggtgg tttgcttgcg ccgctcgcta gttggccgct
     9481 agaattttcg agtccttggc cgctgcgacg tccatgaacg accgtcctcg ctttgcactg
     9541 cgcttcatct cgggcaagta ccagggaggc gagttcccgc ttcgaatgaa tcgggaaatc
     9601 atcatcggtc gttccagcga tctggacatg gtgctcgtcg aggacatggt ctcgcgtcgt
     9661 cacgcgaaga tcagctcgac ggatgccgag gtctacatcc aggacatggg ctcgacgaac
     9721 ggcacgttcg tcaacggcga gaagatcaac ggtcgcgcgc tgcttcacga aggcgaccgc
     9781 atcctggtcg gcacgtcgat catcaaggtc gtcgcggtcg aaggtcaggt cgcccagcag
     9841 agcgaggccg aggcacgccg ccgcctcgaa gccggagcgc agcagcgcca ggcgacgcag
     9901 ggccgcccga tgtcgggcgt catcgaagag atcccgctgc ccgatttgct ccagctcctc
     9961 tcgacgagcc gcaagtccgg cgtcctcgcc gtcaacaacg gcgtctcgat cggcaagatc
    10021 ttcctgcgca agggtcagat ctacttctcg acgatcaacg acgacttcgc ggtcagcccg
    10081 caaaaagcga tctaccgcat gctcacctgg gagacgggca cgttcgagct cgagcccggc
    10141 ggcgagatgc aggtgatgaa cgaagtgcaa gagtcgacgg agggcctcct catggaaggc
    10201 gtccgccagc tcgacgagtt ccgcaatctc cagaagcagc tgccgccact cggctcgccg
    10261 ctcgctgtgc caacgccgct cgccggcaag ttgcgcgacc tctcaccggg cgagctcgac
    10321 acattccaac ttgtcctcga tcacggtcag ctacagaaag tgctcgataa ctttccgggc
    10381 accgacctcg acgcagcgca gaacatcatc caactgatga agcgcgaatt cgtcgtcgtg
    10441 ccgtagcgca ccgacgtgtc tagatcctcc ggttgtgggc ccgggcagcg cgggtaaact
    10501 ggaggcgtgc ctccgagtac cgtgcgcatc gagacgatcg cacccgacgc gctgcgaagt
    10561 ccgtatcgcg gcatcgcgac ggcgcgagag acgctgcggc tgacggtgtc gatcgcgccg
    10621 cgcggaatgc tgccgtgcga gatcatgatc gacgtcgacg atacgagcgt gcaggtctgc
    10681 gatcatgcgt cggggcaatg cttcgcactc ggtgcgatcg agcagctgtt cgtcgtcgag
    10741 agacagcctc ccgcgtggcg gtttctgacg tggttcggcg tcttcgcccg gcggcgtggt
    10801 cgtcccgatc ggctgctgct cgagacgccg gacggcaagg tcgcgcggtt cgtcgagcgc
    10861 acgatcgaag aacgattggg cctgcgcgac gagcccgtgc gtggcgagct gagactcgcc
    10921 atggagtagt gtgatacgcc tgtagcgtga tcagggaggc agcgtcgatc atcttgctcc
    10981 gccgcatcgc ggtcggcgca ggcttcgaag tcttcctcgt tcgccgtcat cgcggctcga
    11041 gcttcatggc ctcggcgtac gtctttcccg gcggcgctgc cgaagaaggt gaagacgccc
    11101 gcaccgcggc agctcgcgag ctgttcgaag aggccggcgt gctcctcgcg cggcagtccg
    11161 aagatcgcga ctccgaaacg ctcgagctca tgagccaagc ggcgatgcgc aaagacgtgc
    11221 tcgaaggcgc gaaggcgagg tcggtgctcg cggcggcggg gctgcaattc tcgacggaga
    11281 atctcgtgcc gtggtcgcac tggatcacgc catcgatcga gccgaagcgc ttctccgcgc
    11341 ggttcttcat ctgcgaattg ccgaccggac aagagccaca gttcgacgcc accgagacgg
    11401 tcgaccaagc gtgggtcacg ccggaagccg cactcgcccg cgcgagcgag ctgcagctgc
    11461 cgcccccgca gatccgcacg ttctgggagc tcgcgcagct cgagtcgatc agcaaggtga
    11521 tcgcggccgg cagagcgcgc gcggagcaac cgcatccgat catgccgcgc ctcatgccgg
    11581 gcactgcgcc tgcgctgctg ctgccgtggg atcccgaata cctcacgaaa ggcacgggcg
    11641 agagcacgcc gctgacgtat caaccagcgt gggcgacggg gccgagccgg ttcattctcg
    11701 acggaaaatc gtggaagcac gtcgccgcac ctggttcgac gagcgcgggc tcgtagtcgc
    11761 gtcacctggc ggtgatcggc gcctgccgtt ctacgcgggt gcgatgcact actggcgcgt
    11821 cgatccgaag cgctgggccg cttgcctgaa atcgatccac ttgctcgggc tgacgtgcgt
    11881 cgagacctac gtgccctggc gagtgcacga gcccgaagcc ggcgcgctcg actggtccgg
    11941 agaacgcgac ctcggcaggt ttctcgacgc tgcgcggact gccggtctct ccgtgatcct
    12001 ccggccgggg ccgcacatca acgccgagct cacgagcttc gggattcccg actgggtgct
    12061 cgcggatccc gattgccaag cgcgcaccgc gcacgggaca ccggcatggc tgcccgcacc
    12121 gccgcgtgca tttccgatcc cgtcgtacgc ctcgacgaag ttccgcgcgc gcgtgaaagc
    12181 ttggtacgcc gaggtcgcga agatcgccgc gccgtatctc gcgcccgacg gacccgtcgt
    12241 cgcgatcggt gtcgacaacg aagcgcagca cttctttcgc ctcggcgcgt tcgacctcga
    12301 ctaccacccg gacgccatcg cgtggtggca cgaggcgagt ggtctcgacg gggagccgcc
    12361 gcgcgaatgg tccgaggagc aggccgcgcg ctgcatcctg tggctcaagc acaaggacga
    12421 atacctcgcg cgtgcgctcg gcgatttttc gcgttcgctc gacgaggagg gctttgcggg
    12481 cgtcgcgcgc ttccacaacc tcccgccggg ccaccacggc ctcaacgaca tccgccagct
    12541 gcagcacgcg atcgcgggtc cggtcggcat cgacgcgtac acgccgcggc ggctgttccc
    12601 cgagctgcgg cagcgcgcgt tctcgctcgt cggcaacgca acgccgattc cgctcgcacc
    12661 cgaagtgggt atcggcttct tcccgtggtt cccgccgctg gacgagacaa ccgacgaccc
    12721 aacgcgcgag cgcgatcatc tgctcacgct gctcgcgtgc ggcgtgcgtg gcttcaacct
    12781 cttcatggcg gtcgagcgcg accgctacta cggcgcggcg atcgctgccg atggcaagct
    12841 cgagccgcac gcccagtgga tcaagccgat cgttgccgcc ctggccgaag tcgattggcc
    12901 cactctgcgg cggcgcccgt tgatcgcagt cgtcgacagc aaagcggatg cgcgcttcgg
    12961 ggtcgccacg aacgtcctcg atcccgtcac cgcgacgctc gccgatgcac tcgatctcgg
    13021 ccccggcggc ttcgccgagc tgggtaccga cgccgccgcg atcgcagcgc gacgctggca
    13081 ccacgcgatc acacgcgcac tcgagctcgc gcaagtgccc tacgcgatcg tcgacgaggc
    13141 cacgcccgaa gacgagctcg caacgtaccg agctatcgtc gcgccgacgc cgggcacgcg
    13201 catcgatcgt ggcctctggc aacgcctgcg tgcgctcgcc gagcacaaac gcgcgatcgt
    13261 cgtcgtcggt ccatcgacgc ccacgcacga cgagctcgat caaccgctcg ccgacgcagc
    13321 gccgaagcgc atcggcaagc tgaaggcagg ctcgctcgaa gacgtcgcgg ggctcgccga
    13381 cgatctcgcg ggcctcgcgg gcgaaacgtc cgatgcgtgg aacgtcgagc gtccggacac
    13441 ggttcgcacg atggcgtact cgacgccgga cggcgcaacg cgcgtcgtgt tcgtcgtcag
    13501 cgatgcagat cgcgcaacga ctgccgtcat caacgcgggc gacggcgcga aggcgctccg
    13561 cgacatctac ggtgagcggc tgcgcgtcga cggcgggcgc gtgaccgtgg cgatgcccgc
    13621 gcgcggcgtc cgcatgctga tcgtggagtg acatgggcgg cgcgaagaag cgggagacgg
    13681 tgcacgtgcc gctgcccgcc tgtgctcgcg aatggagcgc gcgcgaacgt acgatcatcg
    13741 atctgcactt ctactatctg acgaacgtct ggagcctcaa cgactggaag gtctacaacc
    13801 gctatcgcaa gcaccttcct gcggaatctc gcttcgaggt cgatcagctc gtcgatctga
    13861 tgatgaagga cctaccacgc gagatgcgcg ccaagctcga cgcgatcttc gctgtcgtgt
    13921 gggatgcgga ggattggctc gccgtgctcg cgagtgagga gctcgcgcac gatctcgacg
    13981 tcgacgaccg aaacgcgtgg cggcagaaag tcgcggacgt gatgctgaag ctcgcgcgcg
    14041 acggcgcgct gcactaaatc gcgcgttcgg gccgaaacat cgcagccgcc gcggcgatga
    14101 aaccgagcag accgccagcg aggagagcgt gggtgccgat gccgagcgcg atcctgagcg
    14161 tcggttcttc cttgttcatc tcgagcatcg cttcgtgaaa tacgaaccac gcgccgatgt
    14221 tcgcgagcgc cgcaagcatc gcgaggatcg cgagaccgtt gctcatgcga ccacgtacga
    14281 tcgcgatcgt cgcgaagatt gcgagcagga tcgcggggac ccagaagccc accatgatcg
    14341 gcgcgacctc gcggagatcg gcggtgatct ggtcgggcgt cgtgccgcca gcggtcagct
    14401 gcgcttgcgt gtcttggccg gcaaacacca tctggtacgg cgtgatcgaa tcactgcctt
    14461 tttcatctct caccgtcacg aacgggagaa agaagccgac tacggcgagc acggcgcccg
    14521 ccaggcagat ccacttcgcg gccttcacgg cccgagagta ctagcgtagc gagctgatgc
    14581 ggacgggacc ggtcggctct tcgtcgccgg ccgcgaagtc ttccatcgac gcatcgatct
    14641 ggcggacggg gcgcacttcg ccaacgagac gcgaaatagc gtttacgagc tcgccgggat
    14701 cgagcggctt ggggaggaac gcggcggcgc cgatgcgagc gctgatctct ggcgcatgcg
    14761 gggacgcgga aacgagcacg acgggtggtg cgtcgggcag cgcgtcgaag ttcttcgcga
    14821 ggtcgatcac gcccacgcgt gggtcgtacg aatcgacgac gactgcatcg atgccgcctt
    14881 cgctgacgcg aagaaacgct tcacgcggtg tgatcgccac gaggacctcg tggcccgcct
    14941 ccctcatcac caaccccaag cgatcgcgcg tctccggatc ggactcgacg aggagaatac
    15001 tgcccacgtt tggagagtaa ccctaatcgt tgtcgaggtg ggtggtcgca gatggaatcg
    15061 acggcttcca ccgagaggct cacctcggaa aacgccgtat atcgacggtt actgagagct
    15121 cgactcgggc gggctggatt tgcgctttgc gcgctcgtgg ccctcggacc agccgatttc
    15181 cttcgtcagc tgcatcgcgt cttgaagacg gtgaggcatg acgacggcgg caccacgcga
    15241 cggcgtatcg gtgaggagca gctcggcggt ggcgcggcgg tcgccgaggc tgcgcatctc
    15301 ggtgatcgcg gcttggcgat agcggtcggc tttgccgccg aggccagctt tgtcgcagag
    15361 cgaggcgagc agctgctgga cgcgcgcggc gccgaggcgc gagcggacgc gttgcgcatg
    15421 gaacagggcc gcttcggcga gacgcagtgc gcgatacgaa tcaccggctg cgtcgacgag
    15481 ctgcgcttgt gcgcggagca cgcgccagaa cgattcggga ccgtcggtgg cggtgaagcc
    15541 ttcgccgagc gtcgcgaggt cgacgcactc gacgagctca cgattcgcgg tgtctgggtc
    15601 gcccgcgcgc tgcagcgcca tcgcgagatc gagatagagc tcgcagacaa ggttcatgtc
    15661 gcccgagccg atcgcacgac cgacgccgcg gcgaaggcct gcaatcgctc catcgatgtc
    15721 accctcggag agcgcgattg cggcgcccgc gcgatcgatc atcgagacga gcagaggtgc
    15781 ggacgaccaa tcgcgcgctt cggcgaggat gccgcgcgcc agcgctgtat cgccgcgcgt
    15841 acgaaggaca tcggcgagcc gaacggacaa cgcgacgaac tgatgctctg caccgtcttc
    15901 gatgtcgccg gactgcaccg cttggcgaac cgcgacgagc gcacgataga agaactggcc
    15961 ggcgccggct tcgtcgagtt gctctgcagc tgcatcaccg gcggtgcgca ggagctcgat
    16021 cgcatcgcgc gcgtggcccg cgagatcatg atggtgaccg aggaggccga tgtcgttcga
    16081 catcgcttgc acggcagctg cggcggatgc gtggagtgag cggcgaacgt cggcgggcgt
    16141 cgcgtcgtag acaatctcgc gcacgaggcg gctcgcgaac acgagcatgc ccgacggatc
    16201 cttcgtgatg aagccgtgct cttcggcgtc ttgaagagcc ggctcgagct gctcggtcgg
    16261 catcatcgag cgcaggaagt cgaggtgcgg ctcgataccg aggacggcgg cggcctgcaa
    16321 cacatcgcgc gtcgactgcg ggagcatcga cagccgcgcg gcgatcaagt cgggcagcga
    16381 gaccttcgta tcttccgcct tgccgccctc gaggaggtag cgaaccaggt gctcgacgtg
    16441 accgggatat gcgcgcgtcg tctcgaacag ctgttgcacc ggaggcagac ccgagacgcc
    16501 cgccttcgtc agatcatcga cgatcgactt gaggtccgcg gcttcgagcg cgccgatctc
    16561 gaggcgcttg atcgactgtg gccactgctg cgcgaacgcg tgcgtcgcgg tcatcacgat
    16621 cggcggcaat gcgagatcgt tgctctcggt ggcgcggcgc aggatctcca ggctcggcga
    16681 atcgaagcga tcgatgtctt cgcagatgag cgtgactggg ttgtgaacgg cagcgcgctc
    16741 gagcgcacgc aacgacgacc acaccatctc gcgacgacgc acgggcggct ctagctcgag
    16801 cagcgtggtc ggatgaccga agacctgcgc gatgccgggg atgtcgcgct cgttgagacc
    16861 gatcgacagg accgtttcgc ggagctcgag ctcgctcgac accggcggca gctggaggat
    16921 tgcggcgagc aacgagcgaa tcggatagaa cggcgccgcc agaccgctcg ggtccgggac
    16981 gacctggtac atcacgcggc cttcgtcgcc gagacgcgag tacgcgttgc ggagcatcgt
    17041 cgagcgacct gcgccctcgt gcccgacgac gagcaagccg ccattgctgc ccggctctct
    17101 gcgcatgtgt gcgagcagcc cctgcagctc cgggctgcga cccgtgaacg agagcggcag
    17161 cacatccgga agcgtcggag ttgccggaac ctcgaacgtc ttcgacacgc gcgcgcgagg
    17221 agctccgcac tcggggcaaa acttgaacga cgggttcacg gacgcgccgc acgtgatgca
    17281 cttgtccgtc gacggcagac ggtgcatgcg cgcgcggata ccgagcagcg cttcgcgcat
    17341 ttcggccgcc gacgagtagc gatcgctcgg atgcttcgcg agtgccttcg cgatcacagc
    17401 gtcgagctcg cgcggcacgc cgtcgcgacg cgacgtcagc ggcgatggct gcgccttgag
    17461 atggttcgtc aggatctccg tcgtcgagcc ggcgaagaac ggagtctgct gcgcgagcag
    17521 ctcgaacaag atgatgccga ccgcgtagac gtccgatgcg aacgacggcg gcgcgccgct
    17581 gatcacttcg ggtgccatgt actcgggcgt gccgctgatc gatcgatcct cggtctcgcg
    17641 cacgccggtg acgaggcgcg cgatgccgaa gtcgacgatc ttcaccgcgt cgatgttggc
    17701 gcgccgctga tcgacgatga tgttgtccgc tttgagatcg gcgtgtacga cgccggcgag
    17761 atgcgcttct tcgatgcccg cgagcgcctg tgcgacgaga tcgatcacgc gctcgatgct
    17821 gagcgggctc tcgttcacga ggagctgcgt cagcgtcggg cccttgacgt actccatcgc
    17881 gagatagagc aggccgtcgg gtgactggcc gtagtcgatg atcgagacgc agttcggatg
    17941 gttcaggcgc gacgcgctca tcgcttcgcc gcggaagcgc gtgatcatgc gcgggtcagc
    18001 gcagagctcc tcgttgagga tcttgaccgc aaccgtgcgg ccgagcgcca tctggtcggc
    18061 cttgtagacc gcaccgctcc cgccgatgcc gatgcacgcg gtgagcttga acttgtcgcc
    18121 gagaacgcgg ccgacaagct tcaaggactt ctgtacctgg tcctgcacgc tcactggtta
    18181 cccgttcaat accccaaggc ctgtactcgt ctggtgactt cccatcgagt gcctctgtcg
    18241 agcatctcac gaagttcgtt cgattacgac ggcgatccct tggccaactc cgatgcacat
    18301 cgaggccagc gcgaactggt tggacctcat ggagagctcg tgcatgagcg tcagaaccat
    18361 gcgtgctccg gacgcgccaa gtgggtgacc ccacgcgatc gcgccaccgt tcacgttcac
    18421 tttttcaggg tcgagaccga tctggtcgat gcacgggatc gcttgggcgg cgaaggcctc
    18481 gttgagctct gcaagatcga tacgttgcgg agtcactccc gctttctcga gcgctttttt
    18541 gctcgccggg atcggcccca gtcccatgta gctcacctcg acgccggcgg cggcccccgc
    18601 gacgtaacgc gcctttggag tgagcccgaa ttccttcatc gccttctcgc tcgcgacgag
    18661 aaccgctgcc gcgccatcgt ttaggcccga tgcgttgccg gcagtgactg ttccagtttt
    18721 agcaaaagcg ggtttcagtt tttgcaactg ttcgatcgtc gtgtccggac gcggatgctc
    18781 gtccgccgtg acttcgatct tcgcgccggt cttcttatcg gtgcccgtca cgatcggcgt
    18841 gatctcgagt gccatgcgtc cagacgcgat cgccttgccg gcgcgctgtt gcgatgtgat
    18901 cgcaaatcga tcctgcgctt cgcgcgagac gttgcaacgc tccgcgactt tctccgcggt
    18961 ctcgcccatc gcgagcgtgc cgtgtgcttc ttgcatcttc gggttgatga agcgccagcc
    19021 gagcgtcgtg tcgtagatcg tttgattgcc gcgctgaaac gcttccgtcg gtttgcccat
    19081 gacgagcggt gcacgcgaca tctgctcgac gccacccgcg atcacgagat ccgcttcgcc
    19141 ggtcatcacg aggcgggcag cgtccgcgat cgcttgcatg cccgagccgc agagccggtt
    19201 gaccgtcacg ccgggcacgc tgacgggcag ccccgcgagg agcagagcca tgcgagcgac
    19261 gttgcggttg tcctcgccgg cttggtttgc gcagccgagg atgacgtcgt cgatccgctc
    19321 ggtcggaatc ttcgttttct cgacgagcgc gcggatcacg gccgctgcga gatcatctgc
    19381 gcggaccccg ccgagcccgc cgccataccg gccgaatggg gagcgaatac cgtcgacgag
    19441 ataagctggg cgcgtcatcg ggcggaccat acgccgaaaa ctggcgaacg gcaccggcgg
    19501 tccctaccgt cgactcatga ttcacgactt cacgacgttg gccgagcacg acgtgagctg
    19561 gctagcgatc aagcagcccg cggtgatgcg cctcctcgaa gccgagctcg cgacgcccga
    19621 tggtgacgcg ttcgcagcgg gtctcgagct cgcgtgtcgc gtgcttggtg gtcgcacgcc
    19681 gctcggtgat cttcgcctcg atcacaactc gctcgccgcc ggcatgaccg cggtgcgcaa
    19741 cggccgctgc gatcgcgcga tggttcgctc gcttcgcgat cagctcgaag agctgccgtg
    19801 cgtgctgatg cgcgaggagc aagacgcggt ctgcacggtc atcgccgcga tcatctggac
    19861 cgtgctcgat gcgagcgtcc gggatctaga tgatgaagtt ctgaacacgc tcgtcgccta
    19921 agagaagctc gtacgtgcgg cgtccaacgc cgcggccatg tcggctggca gcgctgccga
    19981 tgccacgacg gtcgtcccgc ttggatgcgc gacggtcagc gacgtgcagt gcagcgccag
    20041 ccgatgcagc gcgaagcgct cgcgccagat ccggttgtgc tcgcctttgc cgtagcgcac
    20101 atctccgatc agcggacacg cgatgtgctt caagtgccgg cgaatctgat gcagccgtcc
    20161 tgtctgcggc ctcgcctcga cgagcgcgta gcggccgaac acctcgcgcc gccagatctc
    20221 ggtcaccgcg ggcacacggt ctgcgcccgg cgctttggga atcgcatgat cgatcacgag
    20281 atgctccggt ggatgcccgc gcgtgatcgc gaggtagcgc ttgtcggcgg tcggccacgc
    20341 cgacgcgaac gcagctgccg cttctttgtc gagcgcgaac agcacgagcc ccgatgcgcc
    20401 gcgatcgaga cgattcaccg gatacacgta cgcgttcact tcatcacgca cgcgctgaag
    20461 cagcgcatcg tcggtgtcgt cccatccacg atgcgtcgag agcccgctcg gtttatcgac
    20521 gacaacacat cgctcgtcgc ggtggacgat cagcgtcata gatgctgacg gaagaaatcg
    20581 atctgctgct gctcgcgcgc gaggctgagc tttggatccg gcagcatgtg cgtcgacgac
    20641 agcgtgatga cctcggcgcg cttgccggcg atatagagcg cctcgatcag cgcgagcgta
    20701 tgcgcgaagt gaacgttgtc gtcggtgatg ccgtgaacga tcagcatcgg ccgcgatagc
    20761 ttcgcagcgt acgtgagcac gctcgcgctc gcgtagcccg cctcgttgtt gcgcggctcg
    20821 cgcatgtagc gctcggtgta cgcggtgtcg tagagcttcc agtcgctgac cggtgcgccg
    20881 acgactgcgg ccttgaagac gtcggggcgc tgaagcagcg cgtgtgccga gaagtagccg
    20941 ccgaaggacc agcccgtcac gccgacgcga gccatgtcca tctcgggatg cttcgcgccg
    21001 agtgcttgca gcgcgccgat ctggtcggcg agcggcacgg tgatgaggtc cttcaggatc
    21061 gcgcgttccc aatcgtggcc gcgattcggc gtcccgcgat tgtcgctgcg cacgacgatg
    21121 aagcccgcat ccgcgtacca ctggtccatc acgtagctat cgcgcgacgc gtcgacgtac
    21181 tgcgcgttcg gtccgccgta gaccttcaag atgaccggat acttctgcgc cgtgtcgaag
    21241 ctgcgcggac gcatgatcga cgtgtagtgg gtgcggcctt cgagcgtgac gctctcgagc
    21301 ttcgtcgtcg ggacgagcga cggcgtctcg gccacagacg gcagctcgat ctccttgcca
    21361 tccttcgtca cagcgtaggc gcgcgcgcct ccgttcgtga gcagacacgt aacgacgaga
    21421 atgccgttct cgaagtgcgc atccgtgacg ccgtcgtcct tcgattccca cgcgccgcgt
    21481 ttgatcttcg agcgaagtgg atcagagccc tcgatctcca cgatcggctc atttccgtcg
    21541 agcccgatga gattgcgaaa cttcggaatc gccagcccct cgatagccca ctcacccgat
    21601 ttctcggaca tccacagaaa cgatccgtcc ggtttccaca gcggcgtccc cggcgcgtga
    21661 ccgaaacccg agcgctggcc cggcagcacg atccacgcat cgtcatgctc gctgaggagc
    21721 tccgtggctt tcccggcgtc gtcgaacgcg atgagcttca gatccgtctg ctcgcgattc
    21781 agcacgctga tcgtgagctt gccgttcgcg ggccacgaca cacgcgcgag gtactcgaac
    21841 gcgacgttgt cccacggcag cgcgccgagg tccttaccgt cgatgccgac gcgcgcgagc
    21901 gagaccttcg cgttcttacg tccggcgcgc ggatacttga acggcaccgg cgccttctcg
    21961 ggatgccgcg cgtccgcgac gtagatcgtg tccatctcgc gcgtgtcggt cagctgatag
    22021 acgatctgct tgctgtccgg cgaccaccac cagccgcgat agcgatcgag ctcttcttgc
    22081 gcagcgaagt ccgcgctgcc gtgctcgatc ccttcggcgt gcttcgtgag ctggcgcgcg
    22141 gcaccgccgg cgacgtcgag gatccacatg ctgccgtcgc gcacgaacgc gatgtgcgtg
    22201 ccatcgggcg agagatgcgc atcgaatgcg tcgcccttcg gatcgagcgt ccgcttcgcg
    22261 ccggtcgtgc gatcgagcag caggaactga cccgcgagcg gcgccatcac ccagcggcca
    22321 tcggcgctca catcgacatc gacgatccct cgcgttgcgg tgcggctgcg ttcgcggcgc
    22381 gccttctccg catcggacag gttctcgtcg cccgccgtcg cggagagcag cgtcttgacc
    22441 tggccggtct tcgtatcgag ctcgaacaga tcggccgcga acgacctcgc gggcgtgcgg
    22501 cggaacagca ccgcgccatc cggcgtcacc gcaacggggg cagggcggcc gagcttgaag
    22561 ttgtacgttt cggcggcgtc cgcgaggagc ttctcgtcga gggcgggcca ggtcgtgctg
    22621 gtcgtagtta tcgaagtggt gggcatctcg tggctcacga acggatcgag cggcgacggg
    22681 ttcgagcggc aagcgccggc gagcgcgagc agcggcatca ggcggcgcat cggcgcgcag
    22741 tatgccggtt ttttgccccg gcgcgtcggg tgccggacca ttgcgaatat gcgcaaggaa
    22801 cgtcggcgcc gtcgcgccaa aaccatcacg ttggctctca gttaccaact cgatgactgc
    22861 tgcaaagagg gcggaatcgc gggcatggtc gtcgcagatg gcgatggctt gccgctcgcg
    22921 gcggccggtg atgtgtacgc gtgcgatgaa gtcgcggcgc ggatgtgtca cgtcggatcg
    22981 cgcatcaagg aattcaacgg cacgctgctc aacggcgcgt cgcgctggga tgtgcagatg
    23041 acgaaggtgc aagtcgatgg cagcgaccta ctcgtatgcg ccgtcggtgg tacagcggaa
    23101 gctcgcaaga agcagatcgc gcgcggtgcg gcgggtgcgc tgcgcatcct gaacgcgcgc
    23161 gctgcttagc gacgagtatc tgctagcttt ggggcatgcg cacgctgctt ctcgtcgccg
    23221 tcctcgccgc ctgttcgtcg tctcacaaag gtaacgacgt tcccgacgac gaggcccgca
    23281 agctcctgct cgatcgcaac tggctcgatc gcatgccgga gaccgagcgc gatcgcctcc
    23341 acgtctatcg cttcgtgccg tcgatgggcg gcggcgtatt ccaagatcgc acgctctaca
    23401 aaggcgagtt cgagctgttc cgcttcgaga ccgccgccga cgagattcgc ttcgacttgc
    23461 cggagacgca ccagaaggtc gtcagcaagt tccgcatcga gaaggtcgac ggacccaagc
    23521 cgttcgatct gcgcctcatc atcgctgccg atccgcgcgg cccgcaggtc tactacggca
    23581 tgcggtctga gacggatcgc gatggtgcag gtctcgagcg gcgcctccac ggcagcagga
    23641 ccgagacgac cgcaacgagc cagccgtagt cggcatcacg gccagccgag cacgcgccgc
    23701 cacccgccga cggctcgagg atgcacaggt ggttgcagcc gtcgtcgtcg tcgagcccgc
    23761 cgtcgtcgca ctgctcgcct tcttcgacga tcgagtcgcc gcagctgccg atgtgatcga
    23821 gcccgacgag gcacgcgtcg tcgagcgtcc agccgccgag ctccttgttg aagtcgctcg
    23881 tgaggcccca cgtgatctgc atcgtgcctt cgcgcacgta cggcgtcacg tcgatatcgt
    23941 ggaagcgcca ctcgcgatcc acatgatcga gcgtgccggt cggtgagaag acgttgtgcc
    24001 agaccggctc gtcgttcgcg aagatcgtgg cttggtcata acgcgcatct tcgacggtga
    24061 gccaccgcca gtattggagg tgcacgtgct gatagcgcga gacgtcgacc accggtgtga
    24121 ggatcgatgt cgcgatgctg tcgcgatagc gaccgtcgcc cgtgatgtcc gtgccgagga
    24181 cgctgccgcc cgtcttcgcg aacgaagggt cgccgctgct cggattctgg atcggcgccg
    24241 cccactgcca ttcgatgcct tcgctgctcg actgcgtcca catcggatct gcgtccatgc
    24301 gttcgcacca gatctgcgac ggcacgccgg ccatcagctg gtacagcgga tccgccggat
    24361 tgttcggaaa cacctgcgac gtgccgtcgt cgaactggac gtcgacggag tacgagacga
    24421 cggtaccggt cggctgttcg ggaatgtcac cgctccacgc atcaccgtcg tgcgtgagct
    24481 cgaagacgga tgcgacgccg tcgccggcct tccacgtgac gtacgcatgc gtgaccgccg
    24541 gtgccgcgca cgtggtgccg ctcggcgtcg atgtcatgag agcgacgtgc aagccgctga
    24601 cgaggggctg cgagacggtc gttgtctgat atccaggcac gaggccatgc cggccgaacg
    24661 cattttcgat cgcgcagtag ttcggtgtgc cgtccgcgag atcggcgttg tcgtcatcgg
    24721 cgacgagcgc ggcgtcgaac gtcgtcatga tgtcgtccgc gcgttgcatg acgccggtga
    24781 agatcgcctc ggcctgcgcg acacctgcgg tgtagccgag cttgctgatg agacctttgc
    24841 gcagatccca gagcgcgccg gcgatgatga ggccgctgac gtgatcgtcg aagtcgaggt
    24901 cgacgggata gacgcgttcg aggccgacgg gatcgatgtt ccgcaacggc gattcgtcgg
    24961 cgaggtagat gccgcgcccg atcgtcggat cctctgtgat gttcgcggcg aagaaatcgg
    25021 acagaccttc cgacagcgcg ctctcgaacg caccgacgcc actgataatc gagtgcttgt
    25081 gcagcgcgtg gccgaactcg tggaacacga tgtccgcaat gcgcccggtg ttctcgcact
    25141 tgtcggtgcc gcgatagaga tagacctcgt tgcccgtcga gtacgcgttg cactcgccga
    25201 cggcgttcac gtagaagtcc atcgtcgtgt cgagccacgc cgacgccgcc gggttcacga
    25261 tgcgcgcacg ctccttggcg cggttcgcat agacgaacgt cgacagctgc gcgtcgccga
    25321 gctcgtcact cgagaggtcc cagacgatcg tgctgccggg tgaaacgccg gtgaacgtcg
    25381 ttgtcgccgc cgcgcccgcg ctgttgttga cgcgcacgta gaggccggtc gtcgacggtg
    25441 tgacggtcgc aggtgacgtg cccgcccagg taacgatgcc ggtcgccgtc gttgctcctg
    25501 tcgtgccgtt gatcgtgatc gagtcgcgca cggcgtcgta gtcctgccga gcgcctcgtg
    25561 cgtagcgaac gcccgcgttg aacttcaagg ttgcggtcgc atccatgacg agcttctcgc
    25621 gccccacgat gcggtcgtct gcatcgcggt agacgcgcca gcgctcgggc gtgcgactcg
    25681 aggtctcctc ggtgacctcg gcgatcgtcg acgtgccgcc gcgctgcagc acgacgagct
    25741 cgcctctcgg cgctcgacgc ctcgccacga tcggcgcagc gatatcgtcg atcgcgcggg
    25801 acttcacggc gaacaggcga tcgccatcgc ggccgcgacc gaagacaaca gcgacggtcg
    25861 catcgacgac gcgcaggcct cgccacgtct gctggaacga cacggttcgc agatcgccat
    25921 cgagctgatt tgcgatgagc gtgaagtccg cgagcgtggc tccgctcggc agatgcgccg
    25981 ccacatatcg gcgcgcgtcg cgctccgcga tcgcagcgtc gtcgaccgcg cccgccgatg
    26041 ccacgcgttc cgcacgccac ggacgcgcct cggccgtcgc gccgagcacc gcgcagccga
    26101 tcatcgccgt cgcgaatagc gagcgcgcct tcacgacaag cgaaggcgtg gcgacaagcg
    26161 ggcagcgaga cacgacgaac acgatggcac gagtggcagg cgaggcgcgt acgaagctag
    26221 ccaggctgtg cgccgggtcc cactactcgt cgcatttctt gtagtccgcg cagtccttcg
    26281 cctttgcctg acactccgct tggaccttga gcccggcttt cgtctcgtcg tccatcgcct
    26341 gatacagctc gcgcgcattc ggcatcagct gcgccgtctc gccggtgagc accgcgcgac
    26401 agaggccgcg ccaccgatcg agcgtcagct cgcgcgacag agctgcgccg gagcccgagc
    26461 cgaggtcgag ctcctcgcac ttgtcgaaca tcgccgcggc ttttgcgcag agctcttgct
    26521 ccttcgtcac gccgcccggc ttgcaggccc cgagcgcgat gccaacgccg aaaagcaaaa
    26581 gcgcgctgcg catggccgaa gcctagcgcg agcgcgcagc ggtaactagc gagggttacg
    26641 cgcgagcgac gggcgacgtc ttcgccgtct tgcccgactt cgtcgcaacc gcggcaggcg
    26701 ccttcttcga ggacgacttg ccgttgccgt tgccgttggt cgagacggcg acgccgttgc
    26761 ccgacttcgc gtgatcgcgg agcttgtcga cgagcggcgt ggcttcccac gtgaactcgg
    26821 gtagctcgcg gccgaagtga ccgtacgacg ccgtcttcgc gaagatcggg cggcgcaggt
    26881 tcagctcttg gatcagcatg ccgggtcgcg cgtcgaagac cgccatgacg gccttggtga
    26941 gcttctcgtc cgagacgatg cccgtgccga acgtatcgac gagcatcgag accggctcgg
    27001 cgacgccgat ggcgtacgcg aactgaacct cgcagcgctt cgccaggccc gcggccacga
    27061 cgtgcttcgc gatgtaacga ccgtagtacg cggccgagcg atcgaccttg ctcggatcct
    27121 tgcccgagaa agcgccgccg ccgtggcgag cgtagccgcc gtacgtgtcg acgatgatct
    27181 tgcggccggt gaggccggcg tcgccgtgcg ggccaccgat gacgaagcgg ccggtcgggt
    27241 tgatgtggat cttcgtcttg ttcgtgagga gcttcgggtt cacgacgggc ttgatgacct
    27301 gctcgcgaat cgcttcctcg atcgtcttct gcttgacgtt gtcgtcgtgc tgcgtcgaga
    27361 cgacgatcgc ttcgatgccg gtgatcttgc cgtcttcata acggaccgag acctgcgtct
    27421 tgccgtcggg acggagccag tcgatgcctt tgatctgctt cttgcgaact tcggcgagct
    27481 tcgccgcgag ctggtgcgcc gtttggatcg gcatcggcat cagctctttc gtttcgtcgc
    27541 acgcgtagcc gaacatcagg ccttgatcgc ctgcaccctg cttcgcggga tcggtgccgc
    27601 gatcgacgcc ctgcgcgatg tcgggcgatt gctgcgcgac ggcgaccatg acggcgcacg
    27661 agtcggcatc gaagcccatg tccgagctgg tgaacccgat ctcgcggatc gcgccacgtg
    27721 cgatcttgtt gtagtcgagg ttcgcctttg tcgtgatttc gccgaggagc atcacgaagc
    27781 cagtcttcgt cgcggtttca cacgcaacgc ggctgttcgg atcctgcgcg aggcaggcgt
    27841 cgaggacggc gtcggaaacg gcgtcgcaaa gcttatcggg gtgaccttcg gtcacggact
    27901 cagaagagaa caggtaactg gccatgggtg ccggaagtat ccagcttgcg cgtccgctgt
    27961 caacggatgt tcgaggtacc atagcggaga tgcgcgcatg ggttttcgca tgcttggtga
    28021 ccctcatcgc atgcggtggc aaaccgccga tcccgaaacg cggtgtcgtc gagggagacc
    28081 tcggcgcgtg gaagttccgg agattccaac cagtcctcga cgtggaagtc tgggtcgaga
    28141 acaacaaggc cgaggcctat acggcgagtt acgtcgccga cgacgctgag aagaaggggc
    28201 acgtcgagga caaggacgtc gtgaacgtgt ttgttacacg ctacgagaaa gacgatggcg
    28261 tgctgcgcga gacggtaaag ctcgtacggc gattggcggc agaaggcggt taccaagtcg
    28321 acgagcacaa ggtcggcggc gcacgcgcac tgacgatcaa tggtcacggc gaatcgtggg
    28381 tgatgtgggc ctcgacgaag cacgtcgtca aagtcggtgg ccgtgggcgc gagacggtgc
    28441 cggattccgt cgtatcgagc tacgcgaagc gatacccgtc gaagattcca ggcggtgcgc
    28501 tcgaggggcc gctacctgct ggacccgata atcagggccc ggggcccgaa ggatcgcccg
    28561 agtacgaccc gaacaacccg cacgcggatg ccagcaagat cgacacgtcg aaggtgaagc
    28621 tgccggacac gaaggagaag gacgttcctt ccgacaagcc cgacgacaag aagccggatc
    28681 cgaagaagaa gaagaagtag gctccgcgca tgctgctgag gcagatcgca atcgggtcgc
    28741 ccgaagctgc cgcgctcctc gatcgctcga cgcgcttcga ggacggagtc gacgacacgg
    28801 tcgcaacgat cctgcgcgac gttcgcacgc gtggcgatga ggccgtcgcc gagtacacgc
    28861 ggcgcttcga tcagcgcgag ccgccgtatg aagtgacgcg cgcgaagtgg gacgagctgg
    28921 ccgcaaaggt ctcgccgcgg gtcaaagcgg cgctcgagct cgcggcatca cgcattcgcg
    28981 cgtttcacga gcggcaggcc gagcccgatg tcgacgtgac gctcgacggt gtacggctgg
    29041 agctgcgcgt cacgccgctc gcgcgcgtcg ggctttatgt gccgggcgga acggcgcgct
    29101 atccgtcgag cgtgctgatg acagcgatcc ccgcgaaggt cgccggcgtt cgcgaggtga
    29161 tcatggtgac gccgggtgcg tcggccgaag cgatgctcgc ggcgcggctc gcgggtgtcg
    29221 atcgcgtgtt cgagctcggc ggcgcgcaag cggttggcgc cttggcgtac ggcacagcga
    29281 gcgttccgcg cgtcgacaag atcgtcgggc ctggcaatgc gtgggtcgca tcggcgaagc
    29341 gccaggtcta tggcgaggtc gatatcgatt cgatcgccgg accgtcggag gtgctcatca
    29401 tcgccgacaa cgatgccgat gctgcgtggg tcgccgcgga tctgctcgcg caggccgaac
    29461 atgatcgcga agcacgcgcg atcctcgtga ctacgtcgac gtcgctcgca tcggctgtcg
    29521 ctgcggaggt cgaagcgcag ctcgcgacgt tgccgcgcaa ggcgatcgcc gagcatgcga
    29581 tctcacatca cggtgcggca gtgatcgccg gatcgatcga cgacgcggtc gcgttcgcaa
    29641 accgctacgc gccagagcat ctcgagctcg acgtcgcgga tgcacgcgcg gttgccgctc
    29701 gctgcacgac ctcgggcgcg atcttcgtcg gaaaatggtc gtccgaagcc gctggcgatt
    29761 acgtcgcggg cggcaaccac gtgctgccga cgggcggtgc cgcgcgctat gcctcgccgc
    29821 tcggcgtgca cgacttccgc aagcgcacga gcgtgatcga gtactcgcgc gaagctgcgc
    29881 tcgcgcatgc cgatgagatc gccgcgctcg ctgcggtcga gggcctcgat gcgcatggtc
    29941 gctcggccct gcgccgcaaa ggaggcaagc cttgagcgga gacgacagag gcgacctcgg
    30001 gccgctcgcg cgccgtgtgc ccgaagcact gcggatcggc gtcgcgtatc acgtgccgca
    30061 accgccgaac ctccgcgcaa agctcgacgc gaacgagctg ccgtatccgc tgcccgcgga
    30121 gctgcgggcg cgcctcggtg cagcgctcgc cgaggtggca ctggaacgct atccagatcc
    30181 gaatgcgaga acgctccgtg cgctgctcgc cgaccagctc ggtgtggcgg gcgatcagat
    30241 cacgtttggc aacggctccg acgagtgcat tgcattcttg atcgcggcgt tctcggcacc
    30301 gcgcgcgagc acgcagttcg gtgcggcacg cctccatcgc gaacgcccgg ccgccgtgct
    30361 ctatcccgcc ccttcattcg tctactaccg gatcgcggcg atcgcgcacg gcgtcgagcc
    30421 gatcgaggtt ccgctgacgc cgcggttcga gctcgacgaa ggcgcaatcg tccgcgcggt
    30481 cgagactcac aagccgagcg tcgtgttcct cgcgctgccg aacaatccga cgggcacgct
    30541 ctggcgcatg gacttcgccg ccgagctcgc ggcgcgtcat cgcgacctcg tcgtcgtgtc
    30601 cgacgaggca tacgcggcgt actccgggaa gtcgaaccta cccgcgctcg ccgcacaccc
    30661 aaacctcgtc gtgatgcgaa cgctgtcgaa ggtaggcatg gcaggcatgc gcgttggatt
    30721 cacgattgct gcgcccgcga tcgcggagct gctcgagaag gtccggccgc cgtacaacct
    30781 cagctcgctc gatcagcgcg cggcgacgtt tctgctcacc gaaggtcgtg cgtggtgcga
    30841 ggcccgcgcg gccgaggtcg tcacggaacg ccagcgtctc gccgagaatc tcgctgcgct
    30901 ccgcggcgtc gaagtgttcc cgagcgaggc gaacttgttg ctcgttcgct tcgcgggaga
    30961 tggcaaggcg tcgcgcgttt ggcagggcct cgctgagcgc ggcatcgtcg tgcgcaactt
    31021 cgaccgtccc ggcacaccgc tcgcgggctg cttgcggatc acggtcggca cgcccgatga
    31081 gaacaaactg ctcgtcgagg cgctgcatgc gctcgcgtga gctggcggcg atcgcgacag
    31141 tcgtgtcgct ctgctgtagc ggctgtcaca cgtatggtgg ctcgcgaacc acagcgatcg
    31201 tcggtggcgg cacactcgct gtcggaggtg gcctctgggt cgcgagtcgc acgatagaca
    31261 gcccggacac atcgacgaat ctgaagtggg ctgcgctcgg caccgcatcg ctggggctct
    31321 tgctcgcgat cactggagtg atcggtatgg tcgcgcttcc gaccgacgaa gaggcagcgc
    31381 gcgacctcgc acgcgtgctc gtcagcaagg cgcatcaggg caactgtacg gtggttcgcg
    31441 atcgcgccca cgaggtcgaa gagctcgata cgggcgtcta cgatcgcctc atgctcagcg
    31501 acgacgcggt caagcattgt ctcgcgcagt agctctcgcg ctcgcgctcg cagtagcaac
    31561 gtcgggctgc gcgacgtacg aaggatcgcg caataccgct gtcgccagcg gcgtgtcatt
    31621 tgcggtcggc atgggcacgt ggatcggcgg cagccaggcg gatagcgcac ccacggcggt
    31681 tgcagggctc gcgctcggca cggcatcgat gatggtcgtg atggcgtcgg ccgtcggcat
    31741 ggcgattctg ccgaagcgtg tggagatcgc gctgcggctc gcgcacgagc ttgtcgtgcg
    31801 agcggaagcg ggtgactgcg agacggtcac gctgcgccgc gccgaggtcg aggagctcga
    31861 cgcgctggtt tacgaagtcg tcctcatgga ggatcctgcg atcgtcgaat gcttcggttc
    31921 gtcgccatcg tcgtcgtctc cgtcgtcgtc gccatcggcg ccggctgtgg tcccggttcg
    31981 cctggacccg gcatcgacgt cgaccgtgcg atgagcgaag gcgcggagat cgcatttccg
    32041 cggcactaca acggcgagcc gggcatgcgt gtccagggct tcatcatgcg gacgctcgaa
    32101 gcgatgggcg ttccgtacga gtacatgccg gtcggcaccg tggacgttcc gaggatcgag
    32161 gtcatgggca acgtgtatcg agagcgcgag tcgctgcgcg tcgacgacgc gaacctcgtc
    32221 gtgagattcg ggccgcctgg caaagcgctc ctcgtgatgg cgcactacga cagcgttccc
    32281 ggcagcccgg gcgcgaccga caacgccgcg gccgtcgcga tcgtgctcga gctcgcgcgt
    32341 gtgctgaagg cggagccgcc gcagcgtgcc gttatgctcg tcttcacggc tgctgaagaa
    32401 gtcggccttg tcggagcgga ggcgctcgcg gcgaagctcg gcgacgagat cgactttgcg
    32461 atcgcgctcg atctcatcgg tggcgatggc ccgctcgtcg tcaacggggc gagcatgctc
    32521 gtcggtcgcg cggagctggc gtggttgcgc gatggcgcgg atcgagcagg cgtcgagctg
    32581 cagtttccgc tcgtgcatcg cgtcgtcagc cggtggtggc cacaagcgga gcgctccgac
    32641 cacggacctt tcacgcgtcg tggcgtgcgc gcgatccact tctacaatcg cggcaacgac
    32701 ggcgagtgga tcgatctcgc gtaccacagc gaacgcgaca cgtggccgcg cgtgcatcgc
    32761 gagcaagtcg ctgcggtggg acgcctactg cgcgcgctaa cggcggatga ctccgctcac
    32821 gacgccgtcc ccgcgtacga cggcgatggc tttgtcgtgc ccgtcgtgca ctgggtgatc
    32881 gcgcggtggg tgctcgtcgc gatcgagctc gcgcttgcga ttgtcaccgt cctcgcgctc
    32941 gtgcggcagc gtcgcgtgcg gtcgccgggc gctgggctcc tcatcggcac cgcgagcttc
    33001 atcgtcgcgg tcgtgctcgc gatcatcgcc gagcgtctcg tgctgccgta tcgcggagcg
    33061 tggctcctgt cgccactgcg ctccacgctc gcactcgcgc tcgtgctcgc tggaacgttc
    33121 ggcttgctaa cgcgcgtcgt tgcgcgcttc accgcgtgga ctggatcact tcgctatcgc
    33181 gcattcgcgt cgctcacgtg tctcgcgatc gggctcgcgt ttctcgcgat cggcgcggcc
    33241 gagctcgcgt ggatctggct cgtgcctgcg ctcgtgatcg cgatcgcacc cgcggcggtt
    33301 ggcgtcgtcg catcgctcgt gcccgcgatc ctcgtgctgc atccgtttca gcttcgagag
    33361 gccgcctgga acggttttct gccggcctca ctcccgctcg ccgcggcgat cggattgctt
    33421 ggtgctccaa cgatcgcggc cgtcgcctgg gcgtggtcgt cgcgccgcgt tcgcccggga
    33481 ccgttgggga cactcgtcct gggggtgggg tgtggactgg ccgtcagcct cggaattctg
    33541 ttggctggaa ccgccaaaac tacatgtagc tccgtggaat tcgggcgatt tgggctcgcc
    33601 tgcgaacgcg tctagctatt gcattggtag ggccctgcac cgatcctgaa ggggagctat
    33661 gagccgaatt gtcgtcagtc tttcagttgt tttgatggcc acggcggcga cagcaatctt
    33721 caccgcatgc gacgtcgggt ccgtcgtcca gaattcgacc ggcaccgatg gcggcacgaa
    33781 gatggatggc accggtagcg gcagtggcag cggcagcgga tcggctgcga actgcgaaga
    33841 gatttcagcg accccaccgg atggtcacca caaccccggc cagaactgca tcgcggcggg
    33901 ctgccacctt accggccagc tcggtactaa cgctccgccg ttcacgtacg cgggcacgct
    33961 ctacacgacg gccgccggaa ctgcaccggt cgcaggcgcg acgatcttcg tgaagatggg
    34021 cgcgaccgag aagaaggtcc tctctgcgaa caacggcaac ttctggatgg ggcccgcacc
    34081 cgccggaatc aatgcgccga cgaacgcgat gacggcaacg acgaaggcga gtggctgcgc
    34141 gctcgcaccg aatcacgtgc cgatggtcgg cgctctcgtc caaggcggcg gcaactgcaa
    34201 caactgccat cgcaacggcg gcacgacgac cccgatccac ctgccgtagt tacttcttct
    34261 tcttctgcac ggcgatgagg cgcgcgtagg cctcggcggc cgaggtgcac acctgacgca
    34321 gatcctcgat ctgcagcggt gacagctcct tgccgtgacc atagagcacg ttgacgatgc
    34381 gcttgccgat cgtcacgacg cccgcggtcg cgtggcgcgg ctcggaacag ccgagcactt
    34441 tgtagagata gttgttcacc gtcgacggcg tgaccggtcc actgatgagg ccgccctctg
    34501 ccgcggttgc agcttgcacg acggacggcg catcgagcgg gatgagcaag tgctcgacgt
    34561 acccgcgacc gacgatgttg ccgaacgcct tccagccaaa cgccatctga tcgcgcacgg
    34621 cgaatagcac ggcgacgtcg aagatcagcg atgcgtagcc gagcagcacc tcgacgatgc
    34681 gattgcgatc ggtgagcgtc gcgagctcgg tcagtgcaac ctcgacgctg atcgcgccgg
    34741 ggcgacctgg atcgaccgcc gtcggcacga atgcgggcgg cgtcgttacg cgatgcggtc
    34801 gcagatgggt ttgcgcttcg gcgggcgcgg cgtcatcggc gtcgagcgac tcgagcagat
    34861 cttcggcttc gagctcgagc gcggattcgt cgaacgacga cgtcggagac aacgaggacg
    34921 tcttcgtgct cttgagcatc ggccgcggac cgggcgcagc gaccggcgca gcgggaacgg
    34981 gcggcaagcc tggcagcggc ggcgcgggaa ggccggcatc gccttggctc tcggtgccgc
    35041 gcggcgtatc gccgaacttc atgaagcggg cagggcgggc gacgccgtag tagcgctcga
    35101 cgtagtagta gatgcggact tccggcgcga cgcgcggcag cacgcgatag ccggtgatct
    35161 gcgtcagcga ttcgagcgtc gcgatgtcgt gcgggtcctc gatcgcggcg atcagctggc
    35221 ggtcttggac cacgagcggc acgcacttgt gcttgaacgc cgcggtgggc tggaggagcc
    35281 ggacggcagc gcgtttggct cgctcgagca tggcgccggt cgcgatcggg atgccgagct
    35341 cgaggccgag atagaccgtc agcgcttcga gatcgacgag gccatgctcg acgaggacgg
    35401 ttcccagccg ggcaccatcg cgttgctgat actgcaaggc ctggttgagc tgggcttcgg
    35461 tcaatcgacc gtcgcgcacg agcatctcgc cgagccgcat ccgctagcga cggtagcatg
    35521 gctgtttgcc gcacctggag tttcgggcga gcccggcggg tcgtgctatt gctgaggcgt
    35581 gagggccctc gcgtgctgcg cgcttcttgc gctagccggc tgcggcaaca aggcggacaa
    35641 gcccgccccg gcaccgttgc cgacgccgac cggcaaccca ccgacgggca cacccggtga
    35701 gccggcatcc gttgcccagc ccgatttccc ggaagacacc cggtctctcg agctgacgag
    35761 gaccatcccg gtccggctcg accccggcga cgacggcaag cgaatcggca cgatcgcgat
    35821 cgacacgcgt gtcggctggc agcggacggc caaagccaaa ggctgcagca aggcctgggt
    35881 cgagattcgc ccccgcggct gggtctgcgg cgagtttcta aaggcaagca agctgccgcc
    35941 gcagggccgc gaggtccctc agctagagcg tggtgagatc gtcccgggca tctacggccg
    36001 cgtgactgcc agcgccggtg cgcagggctc ggtcctctat aagttgcaga agccggagga
    36061 caagaagcca aaggcgaagg acaccaagaa gaagggcggc ggtccggtca cctcgcccag
    36121 ccagctcgac gacaagccga aggaagcgcg gatggtcgag gccgatccga tcgtcggcac
    36181 gctgacgatc cgccaatacg aggagctgac cgtcgacggc aaggtctact ggaagatcaa
    36241 tccgaagacg aacgagtacg tcttgaagag cctcgtgaac ctcatgaagc cgtcggactt
    36301 caagggcacg cgcctcggcg acgatacggg ctggaagctg cccgtcgcat tcatctggcc
    36361 gcgcaacaac tggacgcaag cgtggacgta cgcgaagccg aacgcgatca gctatcgcca
    36421 gatcaacgcg cgcctcccgg tcgcgatcct cgagacggcg acgaacaaag cgggaaaggc
    36481 gatcgcatac cgcatcgcag atccgacgcc gcaagctccg ctcggcgaat gggtcaacgt
    36541 cgcggacgtt cgcgtcttcg cccccgtcga tccgccgccg ctgctcacac ccaacgagcg
    36601 ttggatcgac gtcgatctcg acaatcagat cctcgtcgcg ttcgagggca cgacgccggt
    36661 ctacgcgacg atggtgtcga ccggcgcgaa ggacacgccg acgctcacgg gcatgtatcg
    36721 catctggctc aaggcgtccg aagccgacat gaagaacctc aaggccgagg atccgtactc
    36781 cgttgcgacg gtcccgtgga cggagttctt ctactccgag gacgatctcg cgctgcacac
    36841 cgcgtactgg cacgacgcgt tcggcaagac gcgcagccac ggttgcgtga acctcgcgcc
    36901 gcgcgacgcc cgctggctct actactggag cgatccggtc gtaccgccgg gctggaccgc
    36961 gacgagtggt gtcgtcgaag caccgggctc ggtggtgcgc gtgcgttcgg ctgccgatcc
    37021 gtcgccgccg tggcgcggct acgcgaagaa ggttgccgaa gcacgccaga accgcaacgc
    37081 cgaatgacac gccgctaagc cgaccaccgt cggaggcggc gtcgaatcta atcgctcgga
    37141 tccggaaaca aaacacggag ccacgaagca cacgaagccg agagggcagc cgcggatgat
    37201 cgatctccga tctccgatct ctgagctccg atccgatctc cgatctccga gttccatctc
    37261 cgatctcggt gactcggagt cgtcgggcac acgtcctgcc cgtgcgcgcg cccatcaacc
    37321 atctccgctc tcccgttcgg ggctccgtgt gcttcggggc tccctgttga gtcctcgggc
    37381 acacgtcctg cccgtgcgcc ccttcgatcg tctccggcct ccgtgttctc cgtggctccg
    37441 tggctccgtg ttgagtcgtc gggcacacgc ccggcccgtg cgattcgcgc gggcgcaggc
    37501 tgcgcaagcg ctacgccgtg cgctcgtcgc ggacgacggc gagcttcttc ggctgcatcg
    37561 cgaggacctg cttctcggcg gcatccatcg cgtgcacgag ctcggggccg aagatgtcgc
    37621 cgatcgcgtc gcgagcgact tgatagagcg ttggcgcgtc cgggtgatac gcgcacgagt
    37681 agtcgggata atcgtcggag acgaagatcg cgccctcgcc gtacgtcagc gggaacaggc
    37741 ggcagatcgt cggcttcgtg ccgcggaagt cccagccctg ctcgatcgat gcgcggtgga
    37801 tcgcgcagcc tcgacggtcg tgcgtcagga acaggcagcc ctcgttgtgc acggcggtgc
    37861 gaacgaccgt gcccgacggc gtgtccggat cgcgctcggg agccgactcg tcgaaccacg
    37921 gcagattctg gacctccgcg gcaagcacgg ggcgaatcag gtccgcacgc gcgaggatcg
    37981 cgtcgcgctc ggtgaggtcg acgtcgcagc cgtattggca gcaggcgtcg agcttgagct
    38041 tattaccgtc gacgatcgtg cattggtggc tcatgcagtc ggcgacgacg cggcgggtga
    38101 acaccgcgat ctcggcacgc tgccacttgg gatgatcgag ctcgatcgtg ggctcgtcgg
    38161 tcaggaggga catcgttggg tcgcggaatc tagcagtctg cgagcacggt cgcgagccag
    38221 gaatcgaggt cggtgcgcga tccgtcgccg cccgcgtctc ggtcgaccgc gagtgtgagg
    38281 gcggagagcg tcgcctgatc gaggatgcag tcggcatcgg ccgccgcagg gcctcgcgcg
    38341 gtgaagtttt cgcggcagct cgtcacgatc gggagcgatt tctgcgtcag tcggatcggc
    38401 gccccgtgcg agcggcacac gatcgggcga gatgcataca cgaggcagcg gccatcggcg
    38461 tcgagggcaa cacaccgatc gggcagggcg gccgcgatat ttcgcgcgag aaccgcacgg
    38521 cgttcagcgg gccagctcga caattcgtcg cgaatggcct ctgcttcgac ccgcgtgacc
    38581 gacagccgca cgtggcagca gtggctgcac ccggtgtcgc agcgcatgtc gtcgccatgc
    38641 cgttccatgg cgcgtgcgaa gaacccatcc accttcgcgg ccagctcggg gtagcgcgac
    38701 atcggtttca gtgtatgacc ggggcagccg gggttggcca tggacctcaa cgacatcgta
    38761 gtttttacaa aggtcgtcga gaccaagagt tttaccggcg cggctgacgc gctcgggctg
    38821 ccgaagtcaa cggtgagccg caagctcgcg cagctcgaag aacgcctcgg cgttcggctc
    38881 gtccagcgca cgacccgcaa gctcgcgctg accgagatcg gcgaggcgta ttacgagcgg
    38941 tgctcgcgca tcgtcgcgga tgtggcggcg gcagagcagc tcgtgaccga catgcagtcg
    39001 acgccgcgcg gccgcctgcg cgtgacctcg acgatcgatt tctcgacgcg cttcctcggc
    39061 gagatcatcg cggacttcct cgcgcagcat cccgatatca atgtcgagct cgaggcgacc
    39121 gatcgcgtcg tcgatctgat cgacgatggc ttcgatctcg cggtgcgctt cgggccgatg
    39181 ccggaatcga cgctgatcgc gaagcggctc gtcagcctgc acctcatcct cgtcgcatcg
    39241 ccggagtacc tggcaaggcg aaacatgccg aagaccgtcg aggagctcga cgagcacgac
    39301 cacgttctgt tcacgcccgc gtcgcgcaac cagacgtgga cgctcgtcca caacaatggt
    39361 ggcgagcaga cgtacgagtt cggccgcccg gcgcggctcg cgagcaacaa ctacggtgcc
    39421 gtcgtcgacg tcgcgcgcgc gaacggcggc atcgcgctga tctccgagtt catgatcacc
    39481 gacgagctca agaccggccg cctcgtccac gtgctccccg agtggaagac gcgcccgacg
    39541 gatgtccacg ccgtctatcc cgcgcgccag aacgtgccgc ctcggttgac gctgttcctc
    39601 gagcatctgt cgaagtcgct gaacccgccg ccctgggcgc gctgatgccg ctctcgcacc
    39661 gcccgcttgt cgcgatggca gcgctcaacg gtctcgccgc cgtgggagcc ggtgcgttcg
    39721 cagcgcacgg cttgcgctcg cggctcgagc cacgcgcact cgaaatcttc gagaccggcg
    39781 cgcgctatca gatgtatcac gcgctcgcga tgatcgcgtg cggcgcgctc atggtggcgt
    39841 ggccatcggt gtcgcgtggt gcgcgcaatg cgggctggct cttccaagcc ggcatcgcga
    39901 tcttctgcgg cagcctctac gcgctcgcgc tcaccgacgt gaagatgctc ggagcgatca
    39961 cgccgattgg cggcgtgctc ttcctactcg gctggctgtg gctcacgatc tcggcgctgc
    40021 gctcttaacc cgaggttcgc gatacgctcc tggcggtgcc cggggacgac aagacggcca
    40081 gcaatggact gtcgcaggca agtggtagcg acgagacggt cgtcgattca ccgagcgcgc
    40141 gcagccggcc caccccacca ccgcgagagg tgatcgggcg attcacgatc gaccgcgagc
    40201 tcggcgtggg tggcatgggc gtcgtgctgc tcgcgcacga tccaacgctc gatcggcagg
    40261 tcgcgatcaa gctcttgcgt gacgaacgat cggcgaccag taatgcgcgc gtgcggctcc
    40321 ttcgcgaggc gcaggccatg gcccggctcc ggcacccgaa cgtcgtcacc gtctacgaag
    40381 cgggcgtgca tgagggccag gtttatcttg tgatggagat gctgccgggc ggcacgctgc
    40441 gccagcgcct ttccggtgag gtcgcgacgc gcgacatcct ccgcctctac atcgaagcag
    40501 ggcgcggcct cgcagcggcg catcgtgccg ggcttgttca tcgcgacttc aagcccgcga
    40561 acgttctcgt cgacgaagcc gatcacgcga aggtcaccga cttcgggctc gcggtgcagg
    40621 atgattcgac cgagcgtgaa gcgcggccgc gcacgtcgga gcccgacctc gacgcgctcg
    40681 cacacccgct gacccgaacc ggcgatctcg tcggcacgcc tcgctacatg gcaccggagc
    40741 agcacgcggg caaggacacg gatccacgct ccgatcagtt ctcgttctgc gtcgcgctgt
    40801 acgaggtgct ctatcggcag catccgtatc gcgacgaatc gctcgaagcg ctgcgcaaga
    40861 gcatgcgcga tgaagaagca cgcgcgccga agagcacagg gcgtgtgcgc aacgcgatca
    40921 tgcgtggcct ctcgcgcgat ccagcgaacc ggtttgcgtc gatggacgag ctgctcgccg
    40981 agcttgctcc gcggcgatcg cgcgcgggat ggctcgccgg tggagcagcg gcactgctcg
    41041 cgatcggcgc ggtcgcgttc gcgttgtcac ccggacatgc gagcgatcca atcgcgcaga
    41101 ccaagcgcgc cgcgctggca tgcaactgcg agctgctcga cgtgcaggtt ccggcgggtg
    41161 cgcgcgtgat cggcgtgcgc gtggtgggcg cgggcgggct cggtccgctc gtcgttgcag
    41221 cgtggcaacc gacgggacag ctcgcactcg ctcgtggctc gcacgtcatc gaatatctcg
    41281 tcggcgacgc tcgctacgac ttcgcggtga tgtcgcgcgg agaggctcgg acgcgaacgc
    41341 tcgcgttgcc gccgccggag cagcgcacag gttacgtgtt tgtccccggc ggtgaagtca
    41401 cgctgctcgg agacacatct ctcgtcgagc tgcgcccgta cctgatcgag agcacagcca
    41461 cggcggacct cgtctcgtac gccgatgcgc gcaaacgagc gttcgcacaa cacgcgcgtc
    41521 tgccgtttgc ggccgaatgg gagtgggcag caaggctcgg cgtgtttccg cgtgcggggt
    41581 gggagtggac gggaagcgca ttcgcaccgt atccgctcgg ccccatcgct cgcgacgacg
    41641 aagcagcgcc gcgcgaccgt tcggaagtgc gaggtggcag cggtgatctc gcgaagcgcc
    41701 tggcgggtct gcacaccggc cgggccggta gccggctcgc gcgtgatgtg acgtcgagcc
    41761 tcgtgctcga acccgtgacg gtgcagctcg cggaggtccc cgtcgcaggt gactcgacga
    41821 gcctcgaggt cgttgttcca cctgccgagc gcgcgcgtct cgatcggttc ctcacgacgt
    41881 ggcgccagct cgatccgcag ccggctctca tcgtcgaggg cgacacgtgc aacatcacgg
    41941 ggatcgtgta tgcgaatggc gcgtcgccaa cgcacgtcga ggtgaacgcg aacaacactg
    42001 cctcgcggcg cgcgacggtc cgcgtgcatc cagatgtgag ctacacgagc gcgagcaagc
    42061 acgtcacgat ttgcacgaac ccgagcatcg agatcctcga cgcgatctgc ttcaccggtg
    42121 acaagctgca cccaggaagc acgcggctcc tcgaggcgat cgcggcatcg ctctcggcgt
    42181 cgcccacatg gaagagggtt gaggtccagg cctacgccga ttccgattcg gcaacgctcg
    42241 cgcagaggcg cgcggagatc gtgcgtgcgc gcctgatcgc gctcggcgtc gcgagtgaac
    42301 gactcgtcgc gcgcggctat ctcaatgacg gcgacattga cgacccagag catggcctcg
    42361 tcgcagagca gagggccgcg cagccgccag cgggctgtcc tgcgccgagg cccggcgagc
    42421 gcatcgcgtt caagtcactc gacccagcag acgcggccga gtgactcagc cgatcactac
    42481 gtcgttgatc atcacttcga tcggctcgac gcgaatcggc acgtcgagaa actcgcgaat
    42541 ggtgtcgatg ttcgtgagcg tgtgctctga cagcggcgcg agcgtgcgga acttgccgcc
    42601 acccgcgatc gccatcggca gcagcagctg atccgccaag cactcgccga ccggcacgcc
    42661 tgcctcgaga tagtcctcga gatcgtctag cgcgtcgtcg gcgacatctt ccgcgggata
    42721 gcccttgcgc ccgtgggacg tcacgagctc gcgcgcgcca ctcgctagct cgacctcgac
    42781 catgaacacg ttgtgcggcc cgtcgttggg aaactcgcgc agctcacacg tcggatcggc
    42841 cagtcgctcc tgcgcaaccg caagctcgcg gtcaccgaca tggcgcggca gcttcgcgca
    42901 gatcgcggtc gcacggcgcg cgacgatctt gcccgcctct acgagctcga tgcgcttcag
    42961 cttcgagggc tttgtctcca tcacgatgcg gccaccgccg tcgggcatga agccgtgttt
    43021 ctcgagcgtg agtgtcacgt cggcacccat cgcgcgtagc ggcggcatga acacacggtc
    43081 gagaaactcg aacggaggcg cgagtggatt gtgcgtgccc ccggtaatga ccgctttcac
    43141 cggatgcggc gcgaacagcg cgggaatcaa gattgtctga acgacgagcg tcgtcgatcc
    43201 cgcggagccg atatcgatgt gcagctcggt cggccaagct tccgacgggc tcggggtgaa
    43261 cacgagctcg ctcgcgccaa cgtcgagtgc attcgtctgc gcgctcgaga gccgagccgc
    43321 cgcctcgacg cacgcgagat gctggcgctg cagccccggc ttcttgcggc cggcgcggat
    43381 gttcttcatc cgaagcttgc gaccggtaat gaccgagagc gcgagcgacg tgcgcaggat
    43441 ctgcccgccg ccttcgccgc gcgatccatc gatgtcgacg ggcaatctca cgcggtgagt
    43501 ctaccctttg acgcagacga cctggcgcag ctcgtggacg acttcgacga gctcctcctg
    43561 cgccgccatc accttatcga tcggcttgta cgccatcgga gtctcgtcga tcacgctcgc
    43621 gtccttcttg cactcgatgc cctcggttgc cttgatgtga tcctcgacgg agaaccgccg
    43681 cttcgcctcg ccacgcgaca tcgttcgccc cgcgccgtga ctgcacgaat ggaacgactc
    43741 ggggttgccc ttgccgcgca cgatgaagct gcgcgctccc atcgagccag gaatgatgcc
    43801 gaggtcatcc tgccgcgcgc gcacggcacc cttgcgcgtg acgaacacgt ccttgccgaa
    43861 gtgatgctcg cgggcgacgt agttgtggtg gcagttcacc gcaagctcgc cgagcttgaa
    43921 tggcggcagc tccttcttcg cgacgccgac gaccgcgtcc atcatcagct ggcgattcgt
    43981 catcgcgaac ttctgcgccc actcgactgc ctgcacgtag tcggcgaagt gcgtggtccc
    44041 ctcgcgcaga tacgccaggt cgttgtccgg cagattgttg atgtgacgcc gcatgtctcg
    44101 cttggccaga tcgatgaagt agctgccgat gcgattgccc acgccgcgcg agccggagtg
    44161 cagcatgaac caaaccttgt cgcgctcgtc gaggcacacc tcgatgaagt gattgccgcc
    44221 gccgagcgtg ccgagatggt tgacgtcgtt gccgccgccg atgcgcggat gcttgtcgac
    44281 gatctccttg aatgtcgaat cgagatcgcg ccacgcctta ttcgccgcgg tcggcagctt
    44341 gctccacgca ccgcgatcgc cgcgtccacc gttcgacgtg cggccatgcg ggacggcctt
    44401 ttcgatcgcg gtgcgcaacg acttgagcga atcgggcagc tgagctgccg gcagatccgt
    44461 ctcgagcgcg atcatgccgc aaccgatgtc gacgcccaca gccgcgggaa tgatcgcgtt
    44521 ctccgtcggc acgacactgc ccacggtcgc accgatgccg aagtgaacgt cgggcatcac
    44581 cgcgacgtga tgaaacacga tcggcaaacg cgcgacgttc tccagctgac gacgtgcgga
    44641 atcctcgacg ccgacgccac gcgtccacgc cttgatcggc actccagaag tttcgatgag
    44701 ctcgtagtcg gacatggcga gggccagacc tttatcggta atcaccgatg cgccacgagc
    44761 aaagtgaatc ggctcggacg gaccgttcta ttacgacatc cggcgatcgc cgtcagcaaa
    44821 aatctcgtaa ccagctatcg tcacgcatgt tcaaagcgct cctcttcacg atcgcgctcg
    44881 ccgcatgcgg cgggcacaaa gcaaaaccag cagagacggc tcctcctccg gctggtcacg
    44941 agcaaggctc cgacacgtcg ctgacggcgc agcagtgcac ggaccaaggt ggcgaagtcg
    45001 tcggcgacat cggcgatggc gcgacgcaac aacccgacta caagtgtgcg aagagcgggt
    45061 tgccgccggt cggccacatc gccgccgagc ccgggcagcc gcaggcgatc gaaggcgctg
    45121 tgtgctgcaa gtaaactagc ggcagacggc gccgggaccg tcggcgatcg tcgtgcactt
    45181 gagcccgtcg gggcacgctt tgccaccgtc acacttcgtc tcgcagctcg tgaactgcgg
    45241 ccccttcgga ccggcaatgc cgtagtactt cacgcactcg agccccgtcg ggcacgaatc
    45301 gttctcgccg cacttctggc cgcgcgcggt ttccgcggtg acagcggccg aaccgctatc
    45361 gcctgcctcg actcccggga caggctccac cgcggccgag ccagtttgcg tcgacgacga
    45421 cgtttgcgag gtcttcgagc cattcgacga gcacgcggcg aggagcaaca caaatgcaaa
    45481 gcgcttcatg ccgcgaccct agctcaacta cggcttcatt tgctgcgagc cgagcgcgac
    45541 gaacccgggc gtctcgagat tcgcgtactc cgtcgcgatc cactcaggag atagcgcgcc
    45601 cgccgagatc cgcacttcgt cgagcgtacc ttgaaagttg agggtgcccg tcggaccgaa
    45661 cacgagggcc ttgttgttcg tgatgctccc gaacccagtc gtcgacatca tgtctgtaaa
    45721 caccccgtcg cggtacgcga gcatctgttg cgcatcgcga tccatcacgc ccgcgaggta
    45781 cacccacgcg ccgagtgcct cggctccgac cgcgggatct cgatagctcg tgccgtcatg
    45841 cgtcttcatg ttccagtcgg ccgtgccgag gagcacgcag tagcctggat cgccgacact
    45901 cgcgccgcct ttgtagatcg gcgcgtcgaa ggcgcccttg ctcacggcga cgttgaccca
    45961 gagcgagaac gagaacgacg tggtgccgaa gtcgaggctg ccgtcggccg gattggtgac
    46021 ggtcatggag tcgtcgatgc cgtcgaatgc gcgcgcgtga ccggcgattc cgtcgacgac
    46081 cgtcggcgtt gtcagtgggg ctgaggagaa cgcgccgtga ccgttgggcg tgctgtcggc
    46141 tgcaccgatg ccggcgtcga gatgccagat gcccttgaac tgcgacggcc atgtcgcgat
    46201 tgcggtcagc ggctgcatgc cggcgccgta gtacatgaag attgtcgtcg tgccgttcgg
    46261 cagcgtcggg atgcgcaccc acgcttcgag cgcccccgat gcgccatcga acgtgacgag
    46321 ctcgtgatcg agcagcgtgg tgccgtctgc ggcggtgaat gtgatgtcgc ggccgtcggt
    46381 gcgcgcgttg tcgccgagct gagcgtccgc ggtcgtgagg atcgcgaccg gaaagctcgt
    46441 gagcgcagcg gtcagcgatg gcggcgtgat cgcgatcggc ttgcgataga ggaagccagg
    46501 acgccatggc gcgtcggggg catcgacgtc gccgccgctg tccatcggga gccctgcgcc
    46561 gccgagctcg ccatgctcga agttgcaggc gctaaccacc aggcacagcg agccggcaac
    46621 cctgcgcatt cagcggtagc cctgcgcctg caggttgaac agcgtcgcgt agcggccgcc
    46681 ttgcttcacc agctcttcgt gcgagccgcg ctcggtaatc ttgccgtgat cgagcacgat
    46741 gatttggtcg gccatgcgca ccgtcgagaa gcgatgcgag atcacgatcg cgatcttgtc
    46801 ggcggtcagc tcgcggaagc gctcgaagat tttcacttcg gcgtcggcgt ccatcgatgc
    46861 agtcggctcg tcgagcacga ggatgtcagc gtccttgcgc atgaacgaac gtgcgagcgc
    46921 gatcttctgc cactggccga gcgaaagctc gcggccttgc ttgaaccagc gaccgagctg
    46981 cgtgtcgtac gtgtccggca tgtccgcgat gaacggcctg gcgagaccgc gctcggcggc
    47041 gtcttcccag cgagtgcgat cgtcgtacgc gcggtcgtcg ccggcgccga tgttctcgcc
    47101 gactttcagc tgatagcgca cgaagtcttg aaaaatgacg ccgatgcggt gatgcagcgt
    47161 cgtgcgatcc cattccctga ggtcgcggcc atcgagcgtg atgcggccgc cggttggctc
    47221 gtagagacgc gtgagcagct tgatgagcgt ggtcttgccc gagccgttct cgccgacgat
    47281 cgcgagcttg ctgccgggct tgatgtgcaa gttgacgtcg gtcagtgcgt gttcgtgcga
    47341 cccgggatac tcgaacgata cgtgctcgaa gcgcacgcca tcgccgggga tcttgcctgt
    47401 cgtcgcgtcg ccggacggca tcaccgtcgg tgtgtcgagg aactcgtaga ggttcgacat
    47461 gtacaggttg tcctcgtaca tgccgccgac atcgccgagc gcgctcgaca gcgccgactg
    47521 cgcttgcttg aagacgagga cgtacatcgt catgtcgccg aacgagatgg tgttgtcgat
    47581 cgtcgcaaac gcgatccaca ggtacatgcc gtagaacgcc gcttggccga tcgtgccgag
    47641 gatgagtgcc cagatgccgc ggctgatcgt gatcttgcga tcgcctgcgt agagcttctc
    47701 gtagatctcg cggtagcggc gaaggaagcg cgggccgagg ccgaagagct tgacctcttt
    47761 ggcgtatgcg tcctgcgcga tcaccatctc gacgtacatc tgctcgcgcg tctcgggcgt
    47821 gcgccaccgc gagaggcgga atgcatcgcc cgagaacttg agctcggcga caaacggcgg
    47881 aatcgcagca gcgatcaata cgcacagtgc gatcggcgag aacgcggcga gcagaccgcc
    47941 gaggccgatc agcgtgacca tgctctggcc gagctcgaat gcctgcgaga cgagcgacag
    48001 cggccgcgac gacgcttcgc gacgtgcacg cgtcatcttg tcgtagagct cgctgtcttc
    48061 gaattgcgtg agctcgagcg tcagagcctt ctcgaggacg agcacgttga tgcgctggcc
    48121 ga
//
LOCUS       DATLBZ010000126        16031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3851460, whole genome shotgun sequence.
ACCESSION   DATLBZ010000126 DATLBZ010000000
VERSION     DATLBZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16031
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3851460"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VMZ53_06760"
     CDS             complement(<1..471)
                     /locus_tag="VMZ53_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS28189.1"
                     /translation="MAELSGKRALVTGASGGIGAEIAKVFARDGAAVVAIDMGGSEQA
                     SAALAETAEKIGGTALTLDVTADDAVDKITEHLRENYDGRADILVNNAGITRDKLLAN
                     MDEGRWSSVLEVNLASILRMNDVLLGADGLRDGGHVVLVSSIAGIAGNRGQTNYA"
     gene            525..1121
                     /locus_tag="VMZ53_06765"
     CDS             525..1121
                     /locus_tag="VMZ53_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28190.1"
                     /translation="MRNLLLSVAAMLSIAAVACGPSSKEVAGAKSARYQGDKLELFAN
                     VRAATEAKYKITKSDETTLGLQTEPRWFSVEGLAVTPRANDDMTEMPDKSINIALVVT
                     MLPDGDAWLVKVTPVMARYNRGIPKPEQVKEGDASLPGWVDGKVDELSLEIHKKLAKW
                     EVKTVPGQLPPPAPNPDQQPPAQTPPAPEAGSPAPAPQ"
     gene            1134..1793
                     /locus_tag="VMZ53_06770"
     CDS             1134..1793
                     /locus_tag="VMZ53_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28191.1"
                     /translation="MRDVIVDSDERVGDGGFLALRRLRLRNERIDGSVSAEYVCDTVV
                     RPYGQDAVVVAVYAKTDRGIEVLVRHGLRPSLTIGRDPERAPIREDKPGLFLTELVAG
                     IVEDGDRGEMGLRGRAAAEVLEEAGFLVDQDSIALLGAGMYPSPGSLVEKFYFAAVQV
                     DPATQQELAGDGSPMEEGASTTWFLLDDAIAACHAGVLVDLKTELGLRRLREYLRSQE
                     R"
     gene            complement(1790..2305)
                     /locus_tag="VMZ53_06775"
     CDS             complement(1790..2305)
                     /locus_tag="VMZ53_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28192.1"
                     /translation="MRQLLVVVALAGCGHHDSDKRDSGAADDGSMQTSDGNPGDGNGN
                     TGSTSIDGQTVSCYSQAAPATTCTVPAHCCFSNFSAQHDGSCQATACTWGTIECDGPE
                     DCTNNGHCCSTHLSDSNGFIGYRLRCQAAACGAQQEELCHTGGAACGTGSCVTAFGNN
                     NDLPRSLFICR"
     gene            complement(2305..2853)
                     /locus_tag="VMZ53_06780"
     CDS             complement(2305..2853)
                     /locus_tag="VMZ53_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28193.1"
                     /translation="MRNTVLTIATLCGLAACATDTVDEEAADRKRRDAGVQHVVDSGP
                     TSSPDAAPTGGGSTGGGTVSCYTEGFPTNTCSLPTHCCFSNYSSQHNGACTSNSCAWG
                     TIDCDGPEDCAAGQHCCSTQLQNSDGIAGYRLTCQASACGAAPAAEELCHPGGASCSN
                     GGSCVTAYGRNNDLPRSLYICR"
     gene            complement(2973..3767)
                     /locus_tag="VMZ53_06785"
     CDS             complement(2973..3767)
                     /locus_tag="VMZ53_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28194.1"
                     /translation="MAALFSCGDNRAVPKDGNGVGDPQDGSAAGSEGESGSGSGSGSG
                     SGSGSETGSGSGSGIGSAVGSGSGSGCGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSG
                     SGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSSTSIDGQTVACYSQNMPSATCTLPTHC
                     CFSAYSALHEGVCSSPTTSSCVWGTIDCDGPEDCTNGGHCCSTQLIGADGFMGYRLAC
                     QATSCADSVYVEELCHPGGPACDNGGTCVTTLGHNNDLPRSLSICR"
     gene            complement(3849..5255)
                     /locus_tag="VMZ53_06790"
     CDS             complement(3849..5255)
                     /locus_tag="VMZ53_06790"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS28195.1"
                     /translation="MPPDRSASILVVEDDAAMRELLTEELSDAGFVVQAAGSAAGGLE
                     VARSGKFDLVVTDLRMPEMDGFDLIRGVTALPDPPHVVMITAFGSIETAIRAVKLGAY
                     DYITKPFEIEELLLVADKALGERSLKNKVSRLQREVEDKFGLGSIIAKSQSMRDVVSL
                     VQRIASSTASVLVTGESGTGKELIARAIHYNSTRAAGPFVGVNLAAVPEGLIESELFG
                     HKKGAFTDARNDKPGLFVEADNGTIFLDEIGELALPLQAKLLRVLQEHEVRPLGATKN
                     TRVDVRVVAATNKNLEAMLADGSFREDLYYRLNVIHLDLPPLRSRPEDIVPLAEHILQ
                     TLGAKQNPHRRMRLSPEAQHLLLAYHWPGNVRELMNVLERGIALCQGELIADDDLPSH
                     VRERRPADFLGAAVARRMTLAELEREFIERVLEDEAGNKTRAAQRLGLDRKTLYRKLD
                     EYAKQDGADPRASSPGDE"
     gene            5283..6389
                     /locus_tag="VMZ53_06795"
     CDS             5283..6389
                     /locus_tag="VMZ53_06795"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HUS28196.1"
                     /translation="MWAHTGRLPHVLSTRSYHDPVLHEREVSALFAGGWHCIAPLSAL
                     RSSGDFATAEVLGEPVLVRNDGGTIRAFQNVCAHRHSMLTCETRGHAPRLRCQVHGWE
                     YDAEGRSLRIPDAKSFAPLAKGEFCLRQYRAEVLGELVFVSLAAGPPLRESLGEEAAS
                     FIEAACSSSHTLVDVWSHDHAANWKVPIENALESYHVPQVHPRPGRTHSKAADATHTL
                     GDRHTLLVNVTPPAGALLRRLAETWRATPQYRYEHLHAFPNMIVATTDVSTLVHVVMP
                     LSPTRSRSIAYCFAHAGEGRRLVHRAIGPVLGEVIKRYTWKILDEDYRVLPAIQRGLE
                     ASTHAGVIGAREERVHAFQQYVARVHDASVRAVP"
     gene            6386..7861
                     /locus_tag="VMZ53_06800"
     CDS             6386..7861
                     /locus_tag="VMZ53_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28197.1"
                     /translation="MKITGVGFAVAQDEAPIPWPLRESAVSRRYRPVGAFAELVYRAL
                     AECVARGPIVIASCNGKVTTWDDDDWRTSFELAEIVDEQAARAAAERAELIASGKLRS
                     RPEIERRPAAKVFGRAVASAQCVSGMHALYIARSLLAAGHPEVTVLAVDIASAPAHDN
                     FEALRVLTDEPAPYTDRSTGFQLGEAAVALRVALEQDRNTIATDIAGPVLGHDLDGDE
                     ALERCITALLENATARQGKPQPGFVIGQGAGPVAVDKAELAAVSRHVATDAPIASALA
                     TCGHTLGASSLLSVGFAALLGHHFGLDRETRAIAFKSLDANGVALDGRPNGLRRAESS
                     SWIVPVHPLVLCRALGGGCGALVVGATDATSAPQVADWGPRSAPVPLRDETLRRIAAD
                     VDAQRPTQPPDILVVTLECPLPPAARIGKRVLPTSVLEMTPGFIPQLVARAWGYRGPA
                     LCLVGRDPEPLLAALRKTHEHVYRLAIRGMHERDVEWIPRG"
     gene            7836..8588
                     /locus_tag="VMZ53_06805"
     CDS             7836..8588
                     /locus_tag="VMZ53_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS28198.1"
                     /translation="MSNGFLAGKRVLVTGGSGGIGRGICEVIAREGARVAFTFHGNRE
                     GADATLELVKKHGVDGMAIGADLRERDAATKIVAQIESEWGGVDCVVNNAAVSEAVPF
                     VLLDDEQWDDVIALNITGVFRLCRAAVRGMIRQKWGRIVNIGSIAGTRAIASPVHYAA
                     SKGALEGMTHSMAHELGRYGILTNCISAGIFEGGLRSTIPEHHQKRYVDSCALGRIGQ
                     PAECGELAAWLASARNTYLNGAVIVLDGGTLA"
     gene            8599..10113
                     /locus_tag="VMZ53_06810"
     CDS             8599..10113
                     /locus_tag="VMZ53_06810"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUS28199.1"
                     /translation="MEPIPAKKDRDAAFFGFTRSICPQCKKVLDAHIVLKGGQVFMQK
                     KCPSHGAFEVLVSADVDYYMRSLTYTKPGTMPFAFETAVEHGCPSDCGLCEEHLQHTC
                     SPIFEINDKCDLTCPVCIVRNQQGYEVSMEHFKQGVDKLMAKEGELPTLILSGGEPTL
                     HPQFFEFVDYCLRGPHAGRIKRVLVSTHGKKIATNRKFAEKFKASGAYASLQFDTLKR
                     GVYPKLRGVELIDQKMQCIDVIKELDIPMVLVPTISKGDNSDEIGALINFGVELPQCS
                     SIVLQPMAHTGDGGDGYHATPAGHRLTMPEVHALIAEQTDWLKPQHWHPVPCSHPSCY
                     TATYLFRMNDGRYIPLPDFVDVRQYLDAMTNQTIIRTDDALEKMILDSIMSLWSATKI
                     GEDSDVILASLKTFLKDTFNSRNPLTAAEIERRTETRAKAIFIHSFMDAWDLDVARLK
                     KCCTHYVMPDGRLMPGCSYNNLYRHKDARFFPKATPVEPTPIRLEDGKRRLPIL"
     gene            10127..10411
                     /locus_tag="VMZ53_06815"
     CDS             10127..10411
                     /locus_tag="VMZ53_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28200.1"
                     /translation="MVDIAELRQLIVEAAPDRAQAEPALTCADDAQLDSVIPFSSLIV
                     LGVIVAVEDTYGIRVTKKMLESALAGGATLRKLATMIDEARVSKAAGGTR"
     gene            10512..11177
                     /locus_tag="VMZ53_06820"
     CDS             10512..11177
                     /locus_tag="VMZ53_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28201.1"
                     /translation="MRVDVFTRGELDDRLPAAERRELHLLGFEGRRAAEWVAGRAAAH
                     RVLGMVDVLAESDGAPRVAGGAGGASGALSLSHDDEWVAVATAPSGRVAVDLCSRANR
                     DQAAQVLRRLDVFGDPCLAWAALECALKLRRRGVMTLLGDGPAEPRGHIVVEAQGAGA
                     TVRGIGADVHVSWRSCDGYALAWAHEVEAQPVVAVERRPAVKAAARSLALRAARAHRA
                     RLA"
     gene            11286..12059
                     /locus_tag="VMZ53_06825"
     CDS             11286..12059
                     /locus_tag="VMZ53_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28202.1"
                     /translation="MSGCFIAAWAVERDVSRIASDHPALWREIRRFNLPVKLALAAAQ
                     RVLPALESPKTARLIGLAPCRPGSPELRAISRDLDAGFARGSSKNLRVNPIYTLHAID
                     NLGLSALSLRLENREPVSCLGGAAGQAWEALDEAMLLLASGAVNEVLVFGGDQSDASW
                     RGELASGAADPEACGVALVMTAKPQRTKLVGMERVPIEPWAIDGAVVDHAHRRSETVV
                     RPHAARGLAGWLAALQSRYAVPDEDGDGIDRITLITEAL"
     gene            12106..13374
                     /locus_tag="VMZ53_06830"
     CDS             12106..13374
                     /locus_tag="VMZ53_06830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HUS28203.1"
                     /translation="MTRVAITAWGALSPFGVTADALWDGLVSGRSAVKPIAKHVAGGL
                     PVTTGGEVRIETSDRERDLVMSRRSIDDALAMAGLVRAETALFWANGLDTFAHENRRD
                     DRAALVEGVAGDQHGALVEGEARDQRSALVEGEARDQRGELVERSAGACFTAIAREHL
                     RPRRMIATACASATQAIGEAFRAIRAGRVRAAVAGGATVMLTPHYALGFAWLQALAMD
                     VAGEEPGAACKPFDRMRRGFALSEGGAALVLEDMDSARARGARVLGEVRGFGSSQDAY
                     DLNRPPPDGAGAELCMRRALDDAGFGADRVAAINAHGTGTRAGDPAEVAAIRRLLGES
                     WRSTPVTSVKGAIGHPMAAAGALQAIAATESCRRGIVPPTCNLVEPDEDCQLDHVMGT
                     AREVDVPVVLSCSYGMGGQNACVVITRGDL"
     gene            13371..13766
                     /locus_tag="VMZ53_06835"
     CDS             13371..13766
                     /locus_tag="VMZ53_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HUS28204.1"
                     /translation="MKNFDAVAGLVRETLSLGNDIDVKPDQLLFYDLEFTSLDLLDLL
                     FRVEEQLGVAIPEGTIYRHARGDTPDEQFAEKGYLTPLGRERLMTLLSDSPPAIFPDK
                     IHVQALPRYITVGALVRLVDHLQAQAKAS"
     gene            13821..14426
                     /locus_tag="VMZ53_06840"
     CDS             13821..14426
                     /locus_tag="VMZ53_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28205.1"
                     /translation="MFELIQSARFHGLRAEARARVGADWPPVTDHFPGAPILPGSMQI
                     ELCAQLAGPLAERAVKAQHDVERWAFVAMVRNAVFQRPVRLPATIELRAELKRVGATN
                     VRAAAEATTTNVRAAAEATTTNMRAAGEATTAGEATTTGDDAQQLRAVEPMTVHAGVE
                     AIVEGAQVCRAELVIMMLEAEPTWASALAEARARVARWERA"
     gene            14494..15573
                     /locus_tag="VMZ53_06845"
     CDS             14494..15573
                     /locus_tag="VMZ53_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUS28206.1"
                     /translation="MKAIEVVAFGGPEVLRIVERADPTPGPGEVLIRVEAVGLNWSDL
                     LQRAGAYPGGPTPPFIAGQEAAGIVVAHGAGVVASTSGSSMSGSSMSGSSMSSSSMSG
                     SSGSSMSGSSGSSRSGSSSGAVVAPPVGARVCAIGPRLGAELAVVPASMCFAWDASAE
                     QRAALPISLLTAYAALAPWAGRIPTTKAAGAPDTVLVHAAAGALGHIATQVGRKLGMR
                     VIGTASGGKRQFVDADVVCGYDELRVAAPDGVDIVLDGVGGQVFRDSLAVLKPRGVIV
                     VVGASSGEPQLIDPQKLVMRSHTVAGLHLRHLLANPDELARTIDACRALDVTARVTSM
                     PMADIANAHARLQGREIAGKLVLTW"
     gene            complement(15580..>16031)
                     /locus_tag="VMZ53_06850"
     CDS             complement(15580..>16031)
                     /locus_tag="VMZ53_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HUS28207.1"
                     /translation="ARHAIGPLWRWLHQVHHSPARIEVLTAFYKHPLEIVSDSVLASV
                     VLFVVLGVSKESAFLASNVSGVLGLFYHWNIRTPRWLGYIVQRPESHCVHHERGVHAF
                     NYSELPLIDMLFGTFRNPHTFDRECGFGEERERKVAALLLGSDVTRS"
BASE COUNT         2524 a         5297 c         5572 g         2638 t
ORIGIN      
        1 cgcgtagttg gtctgcccac ggttgccggc gatgcccgcg atcgacgaca cgagcacgac
       61 gtggccgccg tcgcggaggc cgtcggcccc gagcaggacg tcgttcatcc gcaggatgga
      121 ggcgaggttg acctcgagca ccgacgacca gcggccctcg tccatgttgg cgagcagctt
      181 gtcgcgggtg atgcctgcat tgttgacgag aatgtcggcg cgtccgtcgt agttctcgcg
      241 caagtgctcg gtgatcttgt cgacagcatc gtcggcggtg acatccagcg tcagcgcggt
      301 gccgccgatc ttctcggccg tctcggcaag ggcagcggac gcctgctctg agcctcccat
      361 atcgatcgcc accacagccg ccccgtcacg ggcgaacacc ttcgcgatct cggcgccgat
      421 gccgccggac gccccggtga ccagcgctcg cttgccgctc agctccgcca tgcggcgaac
      481 ctacacctag cgcaccccca cgccgtgggt gtacgactcg cgccatgcgg aatctcctgc
      541 tgtctgtggc tgcgatgctc tccatcgcgg ctgtcgcgtg tggtccatca tcgaaggaag
      601 tcgccggagc caagtcggcg cgctaccaag gcgacaagct cgagctgttt gcgaacgttc
      661 gcgcggccac cgaggcgaag tacaagatca cgaagtccga cgagacgacg ctcggcctcc
      721 agacggagcc gcggtggttc tccgtcgagg gtctcgccgt cacgccccgc gcgaacgacg
      781 acatgaccga gatgcccgac aagagcatca acatcgcgct cgtcgtcacg atgctgcccg
      841 acggcgacgc gtggctcgtg aaggtcacgc cggtgatggc gcgctacaac cgcggcattc
      901 cgaagcccga gcaggtcaaa gaaggcgatg cgagcctccc cggttgggtc gacggcaagg
      961 tcgacgagct ctcgctcgag atccacaaga agctcgcgaa gtgggaagtg aagacggtgc
     1021 cgggtcaact gccaccgccg gcgcccaatc ccgaccagca gccgccggcc cagacgccgc
     1081 ctgcacccga agctggatcg ccggctcccg caccgcagtg attcatgccg caagtgcgtg
     1141 atgtgatcgt cgacagcgat gagcgtgtcg gcgacggtgg cttcctcgcg ctgcgccggt
     1201 tgcggctgcg caacgagcgg atcgacggca gcgtgtccgc ggagtacgtg tgtgacacgg
     1261 tcgtgcggcc gtatggccag gatgcggtcg tggtcgcggt gtatgcgaag acggatcgcg
     1321 gcatcgaggt tctcgttcgt cacggcctgc ggccatcgct cacgattggt cgcgaccccg
     1381 agcgcgcgcc gatccgcgag gacaagcccg ggctgttcct gaccgagctc gtcgcgggaa
     1441 tcgtcgaaga tggcgatcgc ggcgagatgg gcctccgcgg ccgcgctgcg gcagaggtgc
     1501 tcgaggaggc agggttcctc gtcgatcagg attcgatcgc gctgctcggc gcagggatgt
     1561 atccgtcgcc cggcagtctc gtcgagaagt tctacttcgc ggccgtgcaa gtcgatccgg
     1621 caacgcagca agagctcgcc ggcgatggct ctccgatgga ggagggcgcg agcacgacgt
     1681 ggtttctcct cgatgacgcg atcgctgcgt gccacgccgg cgtgctcgtc gatctgaaga
     1741 cggagctcgg cctgcgccgg cttcgcgaat atctacgcag ccaagagcgc tagcggcaga
     1801 taaataggct gcgtggcaga tcgttgttgt tgccgaacgc agtcacgcag ctgccggttc
     1861 cgcatgccgc gccgcccgtg tggcacagct cctcttgctg cgctccacac gccgcggcct
     1921 gacacctgag gcgatagccg atgaagccgt tgctgtcgga cagatgcgtc gagcagcagt
     1981 gcccgttgtt cgtgcagtct tcggggccgt cgcattcgat cgtgccccag gtgcacgcgg
     2041 tcgcttggca cgagccatcg tgttgcgcgc tgaagttcga gaagcagcaa tgggcaggca
     2101 ccgtgcacgt cgtcgctggc gcggcctggg agtagcagct gacggtctgg ccgtcgatgc
     2161 tcgtcgagcc ggtgttgccg ttgccgtcgc ccgggtttcc gtcggacgtc tgcatcgagc
     2221 cgtcgtcggc ggcgccgctg tcgcgcttgt cgctatcgtg atggccgcat ccggcgaggg
     2281 cgacgacgac gagcagctgg cgcatcaccg gcaaatgtac aacgagcgcg gcagatcgtt
     2341 gttacgtccg tacgcggtca cgcagctgcc accgttcgag cagctcgcgc cgcctggatg
     2401 gcagagctct tcggccgccg gcgccgcacc gcacgcgctg gcctgacacg tcagccgata
     2461 accggcgatc ccgtcgctgt tctgcagctg ggtcgagcag cagtgctggc cagccgcgca
     2521 gtcttcgggg ccgtcgcaat cgatcgtgcc ccatgcgcag ctgttcgacg tgcacgcgcc
     2581 attgtgctgc gagctgtagt tcgagaagca gcagtgcgtc ggcaacgagc acgtgttggt
     2641 cgggaagcct tcggtgtagc agctcacagt gccgccgccg gtggagccac cacccgtcgg
     2701 tgcggcatcg gggctcgagg ttgggccgct gtcgacgacg tgctgcacgc cggcgtcgcg
     2761 tcgcttgcgg tcggcggctt cttcatcgac cgtgtccgtg gcgcacgcgg caagaccgca
     2821 gagcgtggcg attgtgagca cggtgttccg catggttggc ctcatctgcg aggaacactg
     2881 catatgacgg acctcgacgg tgaggcaagc ggcagatgca atcgtgaggc gtgcgcgagc
     2941 gctgtaacgc cgctacgcga tcgcgactcg cgctacctgc agatggagag agagcggggc
     3001 aggtcgttgt tgtggccgag cgtcgttacg caggtgccgc cgttgtcgca cgcgggcccg
     3061 cccggatgac aaagctcttc gacgtagacg gagtctgcgc acgacgtcgc ctgacacgcg
     3121 agccgatagc ccatgaagcc gtcggcgccg atgagctggg tggagcagca atgtccgccg
     3181 ttcgtacagt cttcggggcc gtcgcagtcg atcgttcccc acacgcacga ggatgtcgtc
     3241 ggcgacgagc acacgccttc gtgcagcgcg ctgtacgcgg agaaacagca gtgagtggga
     3301 agggtgcagg tcgcgctcgg catgttctgc gagtagcaag cgaccgtctg cccatcgatg
     3361 ctcgtagagc tgccagatcc gctgccgctt ccgcttccgc ttccgcttcc gcttccgctg
     3421 ccgcttccgc ttccgcttcc gctgccgctt ccgcttccgc ttccgcttcc gctaccgctt
     3481 ccgcttccgc ttccgcttcc ggatccgctt ccgcttccgc ttccacttcc gctaccgcta
     3541 ccgctaccgc ttccgcatcc gctaccgctg ccggatccga ccgccgatcc gatgcccgat
     3601 cccgagcccg atcccgtttc cgatccgctg cccgatccgc ttccggaccc actgccactg
     3661 ccgctctcac cctcgctgcc cgccgcactc ccatcctgcg gatcgccgac gccgttaccg
     3721 tccttcggca cggctcggtt gtcgccgcac gagaacaggg cagccatgct cgcggatgca
     3781 agtacgacca gcaaacgcga ccgcatgtgt cccttcagtg gaccagacat ccgaggctga
     3841 gatccagcct attcgtcgcc gggcgaggac gcgcgcgggt ccgccccatc ctgcttcgcg
     3901 tactcgtcga gcttgcggta cagggtcttg cgatccaggc cgagtcgttg cgcggcacgc
     3961 gtcttgttgc ccgcctcgtc ttcgagcacg cgctcgatga actcgcgctc cagctccgcc
     4021 aatgtcatcc gacgtgcgac cgcagcgcct aagaagtctg ccggcctgcg ctcgcgtacg
     4081 tgcgagggga gatcgtcgtc ggcgatgagc tcaccttggc agagcgcaat gccgcgctcg
     4141 agcacgttca tcagctcgcg cacgttgcca ggccagtgat acgcgagcag caggtgctgc
     4201 gcctcgggtg agagccgcat gcggcggtgc gggttctgct tcgcgccgag cgtctgcaag
     4261 atgtgctcgg cgagcggcac gatgtcctcg ggtctcgatc gcagcggcgg cagatcgaga
     4321 tggatcacgt tgaggcggta atacagatct tcgcggaagc tgccgtccgc gagcatcgcc
     4381 tcgagattct tgttcgtcgc cgcgacgacg cgcacatcga cgcgcgtgtt cttcgtcgcg
     4441 ccgagcggcc gcacttcgtg ctcctgcagc acgcgcagca gcttcgcctg cagcggcagc
     4501 gcgagctcgc cgatctcgtc gaggaagatc gttccgttgt ctgcctcgac gaacaggccg
     4561 ggcttgtcgt tgcgcgcgtc ggtgaacgcg cccttcttgt ggccgaacag ctcgctctcg
     4621 atcaagcctt cgggcacggc cgcgaggttc acgccaacga acggtcctgc cgcgcgcgtg
     4681 gagttgtaat ggatcgcgcg cgcgatcagc tctttgcccg tgccgctctc gccggtgacc
     4741 agcacgctcg ccgtcgagct cgcgatgcgc tgcacgagcg acacgacgtc gcgcatcgac
     4801 tgactcttcg cgatgatgct gccgagcccg aacttgtcct cgacctcgcg ctgcagccgc
     4861 gagaccttgt tcttcagcga tcgctcgccg agcgccttgt ccgcgacgag cagcagctcc
     4921 tcgatctcga acggcttggt gatgtagtcg tacgcgccga gcttcaccgc gcgaatcgcg
     4981 gtctcgatcg atccgaacgc cgtgatcatg acgacgtgcg gtggatccgg cagcgcggtc
     5041 acgccgcgga tgagatcgaa tccatccatc tcgggcatgc gcaggtcggt gacgaccaaa
     5101 tcgaacttgc cgcttctcgc cacctcgagc ccgccggccg cgctgcccgc cgcctgaacg
     5161 acaaagcccg catccgacag ctcttcggtg agcagctcgc gcatggccgc gtcgtcttcg
     5221 acaaccagga tcgacgctga ccggtcaggc ggcacggccc gtttatagcg tatggtcgcc
     5281 tcatgtgggc ccacaccggg cggttgccgc acgtgctgtc gacgcggagc taccacgatc
     5341 ccgtgctcca cgagcgcgag gtgtcggcac tgttcgcagg cggctggcac tgcatcgcac
     5401 cgctctccgc gctgcgctcc tcaggcgatt tcgcgaccgc cgaggtcctg ggcgagcccg
     5461 tcctcgtgcg caacgacggc ggcacgatcc gcgctttcca gaacgtctgc gcgcaccgcc
     5521 acagcatgct cacctgcgag acacgcggcc atgcacctcg gttgcgctgc caggtgcacg
     5581 gctgggaata cgacgcggaa gggcgatcgc tgcgcatccc cgacgccaag tcgttcgcgc
     5641 cgctggccaa aggggagttc tgcctgcgcc aataccgcgc cgaggtcctc ggcgagctgg
     5701 tgttcgtgtc gctcgccgcc ggaccgccgc tgcgcgaatc gctcggcgag gaggccgcct
     5761 catttatcga ggctgcatgc tcgtcgtcgc acacgctcgt cgacgtctgg tcgcacgacc
     5821 atgcggcgaa ctggaaggtg ccgatcgaga acgcgctcga gagctatcac gtgccgcagg
     5881 tacacccgcg cccgggacgc acgcactcga aggccgccga cgcgacgcac acgctcggtg
     5941 atcgccacac gctgctcgtc aacgtgacgc cgccggcggg cgcgctgctg cgccggttgg
     6001 ccgagacgtg gcgggcgacg ccgcagtatc gctacgagca cctgcacgca ttcccgaaca
     6061 tgatcgtcgc gacgaccgat gtctcgacgc tcgtccacgt cgtgatgccg ctctcgccga
     6121 cgcggtcgag gagcatcgcg tactgcttcg cccacgcggg cgaggggcgc cgcctcgtgc
     6181 atcgcgcgat cgggccggtc ctcggcgaag tcatcaagcg ctacacgtgg aagatcctcg
     6241 acgaggacta ccgcgtcctg cccgcgatcc aacgcggcct cgaagcgagc acgcacgcag
     6301 gcgtcatcgg tgcgcgcgaa gagcgggtgc atgcgtttca gcagtacgtc gcgcgcgtgc
     6361 acgatgcgtc ggtccgagca gtgccgtgaa gatcacgggc gtcgggtttg cggtcgcgca
     6421 agacgaagcg ccgattccgt ggccgctgcg cgagtcagcg gtgtcgcgcc ggtatcgccc
     6481 ggttggggcg ttcgccgagc tggtgtatcg cgcgcttgcc gagtgtgtcg cgcgtgggcc
     6541 gatcgtgatc gcgtcgtgca acggcaaggt cacgacatgg gacgacgacg attggcgcac
     6601 gagcttcgag ctcgctgaga tcgtcgacga gcaggcggca cgagccgctg ccgaacgcgc
     6661 ggagctgatc gcgagtggca agctcaggtc gaggcccgag atcgaaaggc gtcccgccgc
     6721 gaaggtattc ggtcgcgccg tcgcgagtgc gcagtgcgtg tccggcatgc atgcgctcta
     6781 catcgcgcgc tcgctactcg ctgcaggaca ccccgaggtc accgtgctcg ccgtcgacat
     6841 cgccagtgcg cccgcgcacg acaacttcga ggcgctgcgc gtgctcaccg acgagcctgc
     6901 gccgtacacc gaccgctcga caggttttca gctcggcgag gcggcggtcg cgctgcgcgt
     6961 cgcgctggaa caagatcgaa atacgatcgc caccgacatc gcaggccccg tgctcggcca
     7021 cgacctcgac ggagacgaag ccctcgaacg atgcatcacc gcgttgctcg agaacgcaac
     7081 cgcgaggcaa ggcaaaccgc aaccggggtt cgtcatcggc cagggagcgg gtccggtcgc
     7141 cgtcgacaaa gcagagctcg cggccgtctc tcgtcacgtc gcaaccgacg cgccaatcgc
     7201 gagcgcgctc gcaacgtgtg ggcacacatt gggtgcatcg agtcttctct ccgtcggatt
     7261 tgccgccttg ctcgggcacc acttcggtct cgatcgcgag acgcgcgcga tcgcgttcaa
     7321 gtcgctcgac gcaaatggag tcgcgctcga cggccggccg aacggcttgc gacgtgcaga
     7381 atcctcgtcg tggatcgtgc cggttcaccc actcgtgctc tgcagagctc tcggcggcgg
     7441 ctgtggagcg ctcgtggtcg gcgcgaccga tgcgacgtcg gctccgcagg ttgccgactg
     7501 gggcccgagg agcgcaccgg tgccgctgcg cgatgagacg ctgcgcagga tcgctgccga
     7561 cgtcgatgcg caacgaccta cgcaaccgcc ggacattctc gtcgtcaccc tggagtgtcc
     7621 cttgccgccg gcggcgcgca tcggcaagcg cgttctgccg acgagcgtcc tcgaaatgac
     7681 gcccggtttc atcccgcagc tcgttgcacg cgcgtggggc taccgcgggc cggcgttgtg
     7741 cctcgtcggt cgcgaccccg agccacttct tgccgctttg cggaagacgc acgagcacgt
     7801 ctatcggctc gcgatccgcg gtatgcacga gcgcgatgtc gaatggattc ctcgcgggta
     7861 aacgggtcct cgtcaccggc ggcagcggcg ggattggccg cggcatttgc gaggtgatcg
     7921 ctcgggaagg cgcgcgcgtc gcgttcacgt ttcacggcaa tcgcgaaggt gcggacgcga
     7981 cgctcgagct cgtcaagaag cacggcgtcg atggaatggc gatcggcgcg gatctacgag
     8041 aacgcgacgc agcgacgaag atcgtcgctc agatcgagag tgagtggggc ggcgtcgact
     8101 gcgtcgtcaa caacgcggcg gtgagcgagg ccgtgccgtt cgtgctgctc gacgacgagc
     8161 agtgggacga cgtgatcgcg ctcaacatca cgggcgtgtt tcgactctgt cgcgcagccg
     8221 tgcgcgggat gatccggcag aagtggggtc gcatcgtcaa catcggctcg atcgcaggca
     8281 cgcgcgcgat tgccagcccg gtgcactacg ccgccagcaa aggcgcgctc gagggcatga
     8341 cccattcgat ggcgcacgag ctcgggcgct acggcatcct cacgaattgc atctcggctg
     8401 gaatcttcga gggcgggctg cgctcgacga ttccggagca tcaccagaag aggtacgtcg
     8461 actcgtgcgc gctcgggcgc attggccaac cggcagaatg tggcgagctt gctgcgtggc
     8521 tggcgtcggc gcgtaacacg tacttgaatg gtgccgtgat cgtcctcgac ggcggcactc
     8581 tcgcttagga tggctacgat ggaaccgatt ccggcgaaga aggatagaga cgcggcgttc
     8641 ttcgggttca cgcgctcgat ctgtcctcaa tgcaagaagg tgctcgacgc gcacatcgtg
     8701 ctgaagggcg ggcaggtctt catgcagaag aagtgtccgt cgcacggcgc gttcgaggtg
     8761 ctggtgtcgg cggacgtcga ctactacatg cgctcgctga cgtacacgaa gccgggaacg
     8821 atgccgttcg cgttcgagac ggcggtcgag cacggctgcc cgagtgactg cggcctctgc
     8881 gaagagcatc tgcagcacac gtgctcgccg atcttcgaga tcaacgacaa gtgcgacctg
     8941 acgtgcccgg tttgcatcgt gcgaaatcag cagggctacg aagtctcgat ggagcacttc
     9001 aagcagggcg tcgacaagct gatggcgaag gaaggcgagc tgccgacgct gatcttgtcg
     9061 ggcggcgagc cgacgctcca tccgcagttc ttcgagttcg tcgattactg cctgcgtggt
     9121 ccgcacgccg gtcgcatcaa gcgcgtgctg gtctcgacgc acggcaagaa gatcgccacg
     9181 aaccgcaagt tcgccgagaa gttcaaggcg tccggcgcgt acgcatcgct gcagttcgac
     9241 acgctcaagc gcggcgtcta tccgaagctg cgcggcgtcg agctcatcga tcagaagatg
     9301 cagtgcatcg atgtcatcaa agagctcgac atcccgatgg tgctcgtgcc gacgatctcc
     9361 aagggcgaca actccgacga gatcggcgcg ctgatcaact tcggcgtcga gctgccgcag
     9421 tgctcgagca tcgtgctgca accgatggcg cacaccggcg acggtggcga cggctaccac
     9481 gcgacgcccg ccggccaccg cctgacgatg ccggaagtcc acgcgctcat cgccgagcaa
     9541 accgattggc tcaagccgca gcattggcat ccggtgccgt gctcccaccc gagctgctac
     9601 accgccacat acctcttccg catgaacgac ggccgctata tcccgctgcc ggacttcgtc
     9661 gacgtgcgcc agtacctcga cgcgatgacg aaccaaacga tcattcgcac cgacgacgcg
     9721 ctcgagaaga tgattctcga ttcgatcatg agcctctggt cggcaaccaa gatcggcgaa
     9781 gactccgacg tgatcctcgc gtcgctgaag acgttcctca aagacacctt caactcgcgc
     9841 aacccactca cggccgccga gatcgagcgc cgcacggaga cgcgcgccaa ggcgatcttc
     9901 atccactcgt tcatggatgc gtgggacctc gacgtcgcgc ggctcaagaa gtgctgcacg
     9961 cactacgtga tgcccgacgg ccggctgatg ccgggctgct cctacaacaa cctctaccgc
    10021 cacaaagacg ctcgcttctt cccgaaggcg acgccggtcg agccgacgcc aatccgcctc
    10081 gaagacggca agcgccgcct gccgatcctc tagcggacgc acacgcatgg tcgatatcgc
    10141 cgagctccgc cagctgatcg tcgaggccgc gcccgaccgc gcgcaggccg agccggcgct
    10201 gacgtgcgcc gacgacgcgc agctcgacag cgtgatcccg ttctcgtcgc tgatcgtgct
    10261 cggcgtgatc gtcgccgtcg aggacacgta cggcattcgc gtgacgaaga agatgctcga
    10321 gtcggcgctc gcgggtggcg cgacgctgcg caagctcgcg acgatgatcg atgaggcacg
    10381 cgtatcgaag gccgccgggg gtacgcgatg agcggtgctg cgatgagcgg tgctgcgatg
    10441 agcggtgctg cgatgagcgg tgctgcgatg agcggtgctg cgatgagcgc gatgcgcggt
    10501 aaggcacgcg cgtgagagtc gacgtgttca cgcgcgggga gctcgatgac cggctgcccg
    10561 ccgccgagcg gcgcgagctg cacttgctcg gcttcgaggg ccggcgtgcc gcggagtggg
    10621 tcgcaggacg cgccgccgca catcgggtgc tcggtatggt cgatgtgctc gcggagtcgg
    10681 atggtgcgcc gcgcgtggca ggtggcgcgg gtggggcgag cggcgctcta tcgctgtcgc
    10741 acgacgacga gtgggtggct gtcgcgaccg caccgtcggg gcgcgtagca gtcgacctct
    10801 gctcgcgcgc gaatcgtgac caagcggcgc aggtgttgcg acgcctcgac gtattcggcg
    10861 atccatgcct ggcgtgggcc gcgctcgaat gcgcgctgaa gctgcgacgc cgcggcgtca
    10921 tgacgctgct gggcgacggc ccggccgagc cgcgcgggca catcgtcgtc gaggcgcaag
    10981 gggcgggcgc gaccgtgcgc ggcatcggcg cggatgtgca cgtgtcgtgg cgctcgtgcg
    11041 atggttacgc gctggcctgg gcgcacgagg tcgaggcgca acccgtcgtc gcggtcgagc
    11101 gcaggccggc ggtgaaggcc gcggcccgat cgctcgcgct ccgcgccgca cgagcacacc
    11161 gagcgaggct cgcgtgaacg cgcgcgcatg gatgcgatat gcggggttcg cgtgaacgcg
    11221 cgcttggctg cgatgttcgt ggcttgcgtg aacgcgcgcg cttggctgcg atatgcgggg
    11281 ctcgcgtgag cggctgtttt atcgctgcgt gggcggtcga gcgcgatgtc tcgcggattg
    11341 cgagcgatca tccggcactg tggcgcgaga tccggcgctt caacttgccc gtgaagctcg
    11401 cgctcgcggc cgcacagcgc gtgttgccgg cgctcgagtc gccgaagaca gcgcgcctca
    11461 tcggcctggc gccatgccgg cccggctcgc ccgagctgcg cgcgatttcg cgcgacctcg
    11521 atgcgggatt tgcgcgcggc tcgtcgaaga acctgcgcgt caatccgatc tacacgctgc
    11581 acgcgatcga taacctcggc ctgtcggcgc tgtcattgcg gctcgagaat cgcgagccgg
    11641 tgtcgtgcct cggcggtgcg gcagggcagg cgtgggaggc gctcgacgag gcgatgctgt
    11701 tgctcgcgtc cggcgcggtc aacgaggtgc tcgtgtttgg cggcgatcag agcgatgcgt
    11761 cgtggcgcgg ggagctggcg agtggcgcgg ctgatccgga ggcctgcggc gtggcgctcg
    11821 tgatgaccgc gaagccgcag cgcacgaagc tcgtcgggat ggagcgcgtg ccgatcgagc
    11881 cgtgggccat cgacggtgcg gtggtcgacc atgcacatcg ccgctcagag acggtggtgc
    11941 gcccacacgc cgcacgtggg ctcgcaggct ggctggcggc gttgcagagt cgatatgccg
    12001 tccccgacga agatggagat ggcatcgacc gcatcacgtt gatcacggag gcgctgtgat
    12061 ggcgcgcgcg atgacacgcg tcgcgatcac cgcgtggggc gtgcgatgac ccgtgtcgcg
    12121 atcaccgcgt ggggcgcgct ctcgccgttc ggcgtcacgg ccgatgcgct gtgggacggc
    12181 ctcgtctcgg gtcgctcggc ggtgaagccg atcgcgaagc acgtcgcggg tgggctgccc
    12241 gtgacgacag gcggcgaagt acggatcgag acgagcgacc gagagcgcga cctcgtgatg
    12301 agccgccgct cgatcgacga cgcgctcgcg atggctggcc tcgtgcgcgc ggagactgcg
    12361 ctgttctggg caaacggtct cgacacgttc gcacacgaga accgacgcga tgaccgagcc
    12421 gcgctcgtcg aaggagtagc tggcgatcag cacggtgcgc tcgtcgaggg agaagcgcgc
    12481 gatcagcgca gcgccctcgt cgagggagaa gcgcgcgatc agcgcggcga gctcgtcgag
    12541 cggtcggccg gcgcatgctt cacggcgatc gcgcgcgagc atttgcggcc gcgccgcatg
    12601 atcgcgaccg cgtgtgcatc ggcgacgcag gccatcggcg aagcatttcg cgcgattcgt
    12661 gcggggcgcg tgcgtgcggc cgtggccggc ggcgccaccg tcatgctgac gccgcattac
    12721 gcgctcgggt tcgcgtggct ccaggcactc gcgatggatg tggcgggcga ggagccgggt
    12781 gcggcgtgca agccattcga tcggatgcgc cgcgggtttg cgctctcgga aggtggcgcg
    12841 gcactcgtgc tcgaggacat ggacagcgcg cgagcgcgtg gcgcgcgcgt gctcggcgag
    12901 gtgcgcggct ttggatcgag ccaggacgcg tacgatctca atcgaccgcc gcccgatggc
    12961 gccggcgcgg agctctgtat gcgacgtgcg ctcgacgacg cgggcttcgg agcggaccgc
    13021 gtggcggcga tcaacgcgca tggcaccggg acgcgcgccg gcgatccggc agaggtcgcg
    13081 gcgatccggc ggctgctcgg cgagagctgg cgctcgacgc ccgtgacgag cgtgaagggt
    13141 gcgatcggcc atccgatggc ggccgccggc gcgctgcaag ccatcgcggc gacggagagc
    13201 tgccggcgag gcatcgtgcc gccgacgtgc aacctcgtcg agcccgacga agattgccag
    13261 ctcgatcacg tgatgggcac cgcgcgcgag gtcgatgtgc ccgtcgtcct gtcgtgctcg
    13321 tacggcatgg gcggacagaa tgcgtgcgtc gtgatcacga gaggtgacct atgaagaact
    13381 tcgacgcggt agccggcctc gtgcgcgaga cgctctcgct cggcaacgac atcgacgtaa
    13441 agcccgacca gctgctgttc tacgacctcg agttcacgtc gctcgatctg ctcgacctgc
    13501 tgttccgcgt cgaggaacag ctcggtgtcg cgattcccga agggacgatc tatcggcacg
    13561 cccgcggcga tacgcccgac gagcagttcg ccgaaaaggg ctacctgacg ccgcttggcc
    13621 gcgagcgact gatgacgctg ctcagcgact cgccgcccgc gatcttcccg gacaagatcc
    13681 acgtgcaggc attgccgcgc tacatcaccg tcggtgcact cgtgcggctc gtggatcacc
    13741 tgcaggcgca agcgaaggcg agctgatggt catcgcgggg gcgcgctgat gttcgcgcgg
    13801 gtcatcgcgg gggcgcgctg atgttcgagc tgatccagtc ggcgcgattt cacggattgc
    13861 gggccgaggc gcgtgcccgc gtgggcgcgg actggccgcc agtcaccgat cattttcccg
    13921 gcgcgccgat tctgccgggc tcgatgcaga tcgagctctg cgcgcagctc gccggcccgc
    13981 tcgccgagcg cgcggtcaag gcgcagcacg atgtcgagcg gtgggcgttc gtcgcgatgg
    14041 tgcgcaacgc ggtctttcaa cgtcccgtac gcctccccgc gacaatcgag ctgcgcgccg
    14101 agctcaagcg cgttggcgcg acgaacgtgc gcgcggccgc cgaagcgacg accacgaacg
    14161 tgcgcgcggc cgccgaagcg acgaccacga acatgcgcgc ggccggcgaa gcgacgaccg
    14221 ccggcgaagc gacgaccacg ggcgacgacg cgcagcaact ccgggccgtc gagccgatga
    14281 ccgtgcacgc gggcgtcgag gcgatcgtcg agggcgcgca ggtgtgtcgc gccgagctcg
    14341 tcatcatgat gctcgaggcc gaacccacgt gggcatccgc gctcgccgag gcgcgtgcgc
    14401 gagtggcgcg gtgggagcgc gcgtagtggt cgcatcccgt cgcccacgcc gcggcggaag
    14461 gaagaaggcg agccacgcgt gtatggtgaa cgcgtgaagg ccatcgaagt cgtcgcgttc
    14521 ggcggacccg aggtgctgcg catcgtcgag cgcgccgacc cgacgccggg accaggcgaa
    14581 gtgctgatcc gcgtcgaggc ggtcggcctc aactggagcg acctcctgca gcgcgcgggc
    14641 gcgtaccccg gcggaccgac gcctccgttc atcgcggggc aggaagccgc ggggatcgtc
    14701 gtcgcgcacg gcgcgggcgt tgtcgcatcg acgagcggtt cgtcgatgag cggttcgtcg
    14761 atgagcggtt cgtcgatgag cagttcgtcg atgagcggtt cgagtggttc gtcgatgagc
    14821 ggttcgagtg gttcgtcgag gagtggttcg tcgagcggcg cggttgtcgc tccacctgtg
    14881 ggcgcgcgcg tgtgcgcgat tggcccgagg ctcggcgccg agctcgcggt cgtgcccgca
    14941 tccatgtgct ttgcgtggga cgcgtcggcc gagcagcgcg ccgcacttcc catctcgcta
    15001 ctgacggcct acgcggcact cgcgccgtgg gcgggccgca tcccgacgac gaaggcagcc
    15061 ggcgcgcccg acacggtgct cgtgcatgcg gcggccggcg cgctcggcca catcgcgacg
    15121 caggtcggcc gcaagctcgg catgcgcgtc attggcacgg caagcggggg caagcggcag
    15181 tttgtcgacg cagatgtcgt gtgcggctat gacgagctgc gcgtggcggc acccgatggt
    15241 gtggacatcg tgctcgatgg cgtcggcggg caggtctttc gcgacagcct cgcggtgctc
    15301 aagccgcggg gcgtgatcgt ggtcgtcggt gcgtcgagcg gcgagccgca gctgatcgat
    15361 ccacagaagc tcgtcatgcg atcgcatacg gtggccggct tgcatctgcg gcatttgctc
    15421 gcgaatcccg acgagctggc gcgcacgatc gatgcgtgtc gagcgctgga cgtcacggcg
    15481 cgcgtgacct cgatgccgat ggcggacatc gcgaacgcgc acgcacgcct gcagggacgc
    15541 gagatcgccg gcaagctcgt cctgacttgg tagcgggcgc tacgaacgcg tcacgtcgct
    15601 gccgagcagg agcgctgcga ccttgcgctc gcgctcctcg ccgaagccgc actcgcggtc
    15661 gaacgtgtgc gggttgcgga acgtgccgaa cagcatgtcg atgagcggca gctccgagta
    15721 gttgaatgcg tgcacaccac gctcgtggtg aacgcagtgc gactccgggc gttggacgat
    15781 gtagccgagc cagcgcggcg ttcgaatgtt ccagtgatag aagagcccga ggacgccgct
    15841 cacgttcgac gcgaggaagg cgctctcctt cgagacgccg agcacgacga acagcacgac
    15901 ggacgcgagc accgagtcgg agacgatctc gagcgggtgc ttgtagaacg cggtcagcac
    15961 ctcgatgcgc gcggggctgt gatggacctg gtggagccac cgccacagcg ggccgatcgc
    16021 atgccgcgcg c
//
LOCUS       DATLBZ010000127         4875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6026105, whole genome shotgun sequence.
ACCESSION   DATLBZ010000127 DATLBZ010000000
VERSION     DATLBZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6026105"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VMZ53_06855"
     CDS             <1..280
                     /locus_tag="VMZ53_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28208.1"
                     /translation="ATLVFDYGMAEPQVRAIEAELWMNNASVAQFHRVALEGGHIGAT
                     KFQVSLPAKDGELRLDVDMEGGVHKSFTRAIHVEEGATVTVPLERDLR"
     gene            370..2424
                     /locus_tag="VMZ53_06860"
     CDS             370..2424
                     /locus_tag="VMZ53_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28209.1"
                     /translation="MRAVLVALFVLAACGDDIRGNISVSVPDAWRPAVAELVSLTPYA
                     GLSIGEDGDFRIEVVDDDTIPAEGYRIDPPSVPVSGSTGSLTWRVHAHDVLGAQYGVS
                     VALENMGFLFRHPFDPLVPFAPKAGPTLDNNVHQPQIAVRALHLHTLHPIEAYFAFIE
                     PSPGSTNDAHRIIDWIIKNKGNYVQWAELDDIQRDPARYEKWKPFVQEILAYAHMRGV
                     RVGVSFQLFGMSNLQNGFDLRDLENDDEPLHDAVAGRLSLLTKDLPFDSYDLTYGEFF
                     NSQPQDFIDATNEVVATLRELAPAAEAHTLIHVGATQRVQYMGRDLLYYFLVQYADPT
                     IIPDIHTVMYFNLYDPTVGAYQHTDFSEHRQYLLDRVCAGKKTGYHPETGYWVAFDNS
                     LPMYMPLYVYSRWRDLDGIKKDGCGNALYEHLIFSTGWEWGYWLHDVAALRSSYELPD
                     SYGALVKEAFGTDLGPDAADLVVELAEEQKDALITHGLSGYLAGRDIAIDLGRALNPP
                     IISQPDRISFDQLVTMMVDMMPVDIAAFEANVMAPLKAHAGRLDEIAGKMRDLDLPDT
                     RWGRELRDGIEIDVDRTWFVVHNYEAVLAHIHGDDAAAQAAYDLAADDMASAKTTIAR
                     RHKDLHDTHGSRITARTSNYTFYQYGYLNNADTQCFWRREYAQVGAILGNTTETPPGC
                     LY"
     gene            complement(2421..3800)
                     /gene="rlmD"
                     /locus_tag="VMZ53_06865"
     CDS             complement(2421..3800)
                     /gene="rlmD"
                     /locus_tag="VMZ53_06865"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HUS28210.1"
                     /translation="MNRTRCEVTASSLDDAGLGVGTADGGLRVHVQDLLPGERAEVAV
                     DHSSPHKPEAWGRIVRRIGKTSEERVEPACPAFGRCGGCVWQHLAYPAQLAAKRARVV
                     EALGNVGVPIGDVRPSPSLLGYRNKGKYVVGRLGNEAIVLGAYAPRSHNVIDTIGCRV
                     VAPVIDEVATWVRGAAERAGLVPYEEATRAGELRYVVLREAGGDVMVALVVTSSAPRA
                     KLEKVASALSKHPAVRGLVSIVNDRRDGAIIPSGSSSQVLYGHGHLVEELAGVKLVVG
                     AAEFLQVNRPQATNMYERVAQLAAETHGTDLKDADALRGVKAVDLFAGLGGFGLHMAR
                     AGAEVVAVEIDRDAISQLRKAAQRASLALTPIAGDAGDLPAEIHAQLGTPSVVVVNPP
                     RKGLSEGARRLLGDLGAATVIYVSCGPESLGRDLAVLAEQGWKPDVIEPFDLMPGTAQ
                     VETIVRLRR"
     gene            3830..>4875
                     /gene="mnmA"
                     /locus_tag="VMZ53_06870"
     CDS             3830..>4875
                     /gene="mnmA"
                     /locus_tag="VMZ53_06870"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HUS28211.1"
                     /translation="MSGSTPLVVAALSGGVDSATAAALLVEQGHRVVGMTMRLYDARG
                     TATSSGGRCCGPRDIEDARAVCAHLGIPHYVIDLAAEFGDAVVDDFVEAYLAGETPNP
                     CVKCNQHIKFTPLLQRARAIGADVLVTGHYAQIIDAADGPGLFRGLDAGKDQSYFLFS
                     MPGHELSSVRFPLGGMTKDEVRNHALRLGLPNAVKPESQEICFVPDGDYAGFVAAAAL
                     KRGRSLPTSGKVVRDDTREVLGQHEGVHRYTIGQHRGLGNLTTKEKLFVTAIDPAAGE
                     VRVGPREAAERHSLTIRDLRWLARPSSSFRAAVQVRHRGTPIDADVAIEGDRATVRLE
                     QPTVAAPGQAAVIY"
BASE COUNT          827 a         1681 c         1554 g          813 t
ORIGIN      
        1 cgcgacgctc gtcttcgact acggcatggc cgagccccag gtacgcgcca tcgaagccga
       61 gctctggatg aacaacgcca gcgtcgcgca gttccaccga gtcgcgctcg agggcggcca
      121 catcggcgcc accaagtttc aggtctcgct acccgcgaag gatggcgagc tgcggctcga
      181 cgtagatatg gagggcggcg tgcacaagtc gtttacgcgc gcgattcacg tcgaagaagg
      241 ggccaccgtc acagttccgc tcgagcgcga tctgcgctga cgagctggaa gctggagttg
      301 ccagcgcctg gcgtgaacag gctcgaactt ggcgtttgcg gatgtcgctc ctcggatatg
      361 ctgcgcggcg tgcgtgcagt cctcgtagcg ctctttgtcc tcgcagcgtg cggagatgac
      421 atccgcggca acatctccgt ctccgtgccc gatgcgtggc ggcccgccgt ggcggagctc
      481 gtttcgctga cgccgtacgc gggactctcg atcggtgagg acggcgattt ccgcatcgag
      541 gtcgtcgacg acgacacgat tcccgcggag ggctatcgca tcgatccgcc gtcggtgccg
      601 gtcagtggca gcacggggtc actgacctgg cgcgtgcacg cgcatgacgt cctcggcgcg
      661 cagtacggcg tctcggtcgc gctcgagaac atgggctttc tgttccgtca tccgttcgac
      721 ccgctcgtgc cgttcgcgcc aaaggcggga ccgacgctcg acaacaacgt gcatcaaccg
      781 cagatcgcag tgcgggcgct ccacctgcac acgctgcatc cgatcgaggc gtacttcgcg
      841 ttcatcgagc cgtcgccggg cagcacgaac gatgcgcatc gcatcatcga ttggatcatc
      901 aagaacaagg ggaactacgt ccagtgggcg gagctcgacg acatccagcg cgatcccgcg
      961 cgttacgaga agtggaagcc gttcgtgcag gagatcctgg cgtacgcgca tatgcgcggc
     1021 gttcgcgtcg gcgtgagctt ccagctcttc ggcatgtcga atctgcagaa cggctttgac
     1081 ctgcgcgacc tcgagaacga tgacgagccg ctgcacgatg ccgtcgccgg gcgcttgtcg
     1141 ctgctcacca aagatctgcc attcgactcg tacgacctga cctacggaga gttcttcaac
     1201 tcgcagccgc aggacttcat cgacgcgacc aacgaggtcg tcgcgacgct ccgcgagctc
     1261 gcaccggccg ccgaagccca tacgttgatc cacgtcggcg cgacgcagcg cgttcagtac
     1321 atgggccgcg acttgctcta ctacttcctc gtccagtacg cggacccgac gatcattcca
     1381 gacatccaca ccgtgatgta cttcaacctg tacgacccga ccgtgggcgc gtatcagcac
     1441 acggacttct ccgagcatcg ccagtacctg ctcgaccgcg tgtgtgccgg caagaagacg
     1501 ggctaccacc cggagaccgg ctactgggtg gcgttcgaca actcgctgcc gatgtatatg
     1561 ccgctctacg tgtacagccg ctggcgcgat ctcgacggca tcaagaaaga tggctgcggc
     1621 aacgcgctct acgagcacct gatcttctcg acgggctggg agtggggcta ctggctgcac
     1681 gatgttgcag cgctgcgctc gagctacgag ctaccggatt cgtacggcgc gctcgtcaag
     1741 gaagcgttcg gtacggatct cggaccggat gcggcggatc ttgtcgtcga gctcgccgag
     1801 gagcagaagg acgcgctgat cacgcacggg ctatcgggct atctcgccgg ccgcgatatc
     1861 gcgatcgatc tcggtcgcgc gctcaatccg ccgatcatct cgcaaccgga tcgcatctcg
     1921 ttcgatcagc tcgtcacgat gatggtcgac atgatgcccg tcgatatcgc cgcgttcgaa
     1981 gcgaacgtga tggcgccgct gaaggcgcac gcaggccggc tcgacgagat cgccgggaag
     2041 atgcgcgacc tcgatttgcc cgacacgcga tggggcagag agctgcgcga cggcatcgag
     2101 atcgacgtcg atcgcacgtg gttcgtcgtc cacaactacg aagcggtgct cgcgcacatc
     2161 cacggtgatg acgccgccgc gcaggcggca tatgaccttg ccgcggatga catggcgtcg
     2221 gccaagacga ccatcgcgcg ccgtcacaaa gacctgcacg acacgcacgg cagccgcatc
     2281 accgcgagga cgtcgaacta cacgttctat cagtacggct atctcaacaa cgcagacacg
     2341 cagtgcttct ggcgtcgcga gtacgcgcag gttggtgcga tcctcggcaa tacgaccgag
     2401 acgccacccg gctgtctcta ttaacgacgc agccgaacga tcgtctcgac ctgcgcggtg
     2461 ccgggcatca gatcgaacgg ctcgatcacg tcgggcttcc agccctgctc ggcgaggacg
     2521 gcaagatcgc gaccgagcga ttcgggaccg cagctgacgt agatcaccgt cgctgcgcca
     2581 aggtcgccga gcaaacggcg ggcgccctcg gagaggcctt tccgcggcgg attcacgacg
     2641 acgacgctcg gcgtgccgag ctgcgcgtga atctcggcgg gcaagtcgcc agcgtcaccc
     2701 gcgatcggcg tgagtgcgag gctcgcgcgt tgtgcggctt tgcgcagctg cgagatcgca
     2761 tcgcgatcga tctcgacggc gaccacctcc gcgccggcgc gtgccatgtg cagaccgaat
     2821 ccgccgaggc ccgcgaacaa gtcgacggcc tttacgccgc gcaacgcgtc tgcgtctttg
     2881 agatcggtgc cgtgcgtctc tgcggcgagt tgcgcgacgc gctcgtacat gttcgtagcc
     2941 tgcggccggt tgacctggag aaattcggcg gcgccgacga cgagcttgac gcccgcgagc
     3001 tcctcgacga gatgaccgtg accgtacagg acctgcgagc tcgagcccga cgggatgatc
     3061 gcgccatcgc ggcgatcgtt gacgatcgag acgaggccac gtacggctgg gtgcttcgag
     3121 agcgcgctcg caaccttctc gagctttgca cgcggggccg atgacgtgac gacgagcgcg
     3181 accatcacgt caccgcctgc ttcgcggagc acgacgtacc gcagctcgcc cgcgcgcgtc
     3241 gcttcttcgt agggcaccaa gcccgcgcgc tcggccgcgc cgcgcaccca cgtggcgact
     3301 tcgtcgatca ccggcgcgac cacgcgacaa ccgatcgtgt cgatcacgtt gtgactgcgc
     3361 ggtgcatacg cgccgagcac aatggcctca ttccctaacc gtccgacgac gtacttgcct
     3421 ttgttgcggt agcccagtag cgacggcgac ggccgcacgt caccgatcgg cacgccgaca
     3481 ttgccgagcg cttcgaccac gcgcgcacgt ttcgccgcga gctgggcagg gtaggcgagg
     3541 tgctgccaca cgcagccacc gcacctgccg aacgccgggc atgccggctc gacgcgctcc
     3601 tccgacgttt tcccgatccg ccggacgatc cggccccacg cctcgggctt gtgcgggctg
     3661 gaatggtcga ctgccacctc cgcccgctcg ccgggcaaga gatcttgaac gtggacccgc
     3721 aggccgccgt ccgccgtgcc gacgccaaga cccgcgtcgt cgagcgagct tgcggtgacc
     3781 tcgcagcggg tcctgttcac gccctcacct tagcgctacc ctgcgccgca tgtctggcag
     3841 tacccctctc gtcgtcgctg ctctttcggg cggcgtcgat agtgcgaccg cagcggcgct
     3901 gctggtcgag caggggcatc gcgtcgtcgg catgaccatg cggctctacg acgcgcgcgg
     3961 cacggcgacc tcttcgggcg gtcgctgctg cggtccacgc gacatcgagg acgcgcgggc
     4021 ggtctgcgcg cacctcggca tcccgcacta cgtgatcgat ctcgccgccg agtttggcga
     4081 tgccgtggtc gacgacttcg tcgaggcata cctcgcgggc gagacgccga acccgtgcgt
     4141 gaagtgcaac cagcacatca agttcacgcc actcttgcag cgcgcccgcg ccatcggcgc
     4201 cgacgtgctc gtcaccggcc attacgcaca gatcatcgat gccgccgacg gccccggtct
     4261 gttccgcggc ctcgatgccg gcaaggatca gtcgtacttc ttgttctcga tgccagggca
     4321 cgagctgagc tcggtccggt ttcccctcgg cggcatgacg aaggacgagg ttcgcaacca
     4381 cgcgctccgg ctcggcctcc ccaacgccgt gaagccggag tcgcaggaaa tctgctttgt
     4441 ccccgacggc gattacgccg gatttgtcgc cgccgcagcg ctgaagcgtg gccgctccct
     4501 gcccacgtcc ggcaaggtcg tccgcgacga cacgcgcgag gtcctcggcc agcacgaagg
     4561 cgtgcaccgg tacaccatcg gccagcaccg cggcctcggc aatctcacga ccaaggagaa
     4621 gctgttcgtc acggcgatcg accccgcggc aggcgaagtt cgggtcggcc cgcgcgaggc
     4681 ggcagagcgc cacagcctga cgattcgcga tcttcgctgg ctcgcccggc cctcgagctc
     4741 gttccgcgca gcggtccagg tccgccaccg cggcaccccg atcgatgcgg acgtcgcgat
     4801 cgaaggcgac cgcgccaccg tccgtctcga gcagccgacc gtcgctgccc cgggccaggc
     4861 cgccgtgatc tacga
//
LOCUS       DATLBZ010000128        16655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1618591, whole genome shotgun sequence.
ACCESSION   DATLBZ010000128 DATLBZ010000000
VERSION     DATLBZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16655
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618591"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..137
                     /locus_tag="VMZ53_06875"
     tRNA            63..137
                     /locus_tag="VMZ53_06875"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:95..97,aa:Asn,seq:gtt)
     gene            complement(252..506)
                     /locus_tag="VMZ53_06880"
     CDS             complement(252..506)
                     /locus_tag="VMZ53_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28212.1"
                     /translation="MDPQTDSASARSDAGAQRRWLIGIAITVVFGIFGAVMALLAYTN
                     SSSAPPSRVPGPSRASEPATTPPSPDEPRNKGHGKDHGRK"
     gene            690..863
                     /locus_tag="VMZ53_06885"
     CDS             690..863
                     /locus_tag="VMZ53_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28213.1"
                     /translation="MIRKYTGNKKSIEARSTDNGRTWSVKLFDTGRVTEYTGGSLAEV
                     DALAAKHGMKLDR"
     gene            complement(885..1874)
                     /locus_tag="VMZ53_06890"
     CDS             complement(885..1874)
                     /locus_tag="VMZ53_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28214.1"
                     /translation="MPDSAASRVLARLRAVAAPGATVEGYGSSIYAPDHASDVDVLVT
                     NDNPARLATALGFELLPTIPPRLHGTLDGMPVDVTVVTGDDDVSRRMSSGPRDAALLA
                     SQLRDNARDEVFQLAWPHVRQFVRARALGHNGLGWFGSFGWAILLAVPLVTELRTVAP
                     GAALPAWLRWLSALALGSRVGFDGVRAGQAEPFFLAAPAPPTRDVAKLSRRSTLTLFA
                     EVRAAVIAIGDAASDTDAIERIKDISDDPPAGTTLVIAGDDEHTRGRYDGIARGLLRE
                     LETLGAIRSWGRFDLTGDGAWQHRITVPTPRTKSARELIENWLSLNRLDAALE"
     gene            1907..2191
                     /locus_tag="VMZ53_06895"
     CDS             1907..2191
                     /locus_tag="VMZ53_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28215.1"
                     /translation="MHMLLVKFHSGLDDKTVARLLEERLPLFRAVPGLVQKYYAREES
                     SGDYVGCYLFESKQAVLDYRASELAKSIPPTYAVEGVAGIEMLEVLFPLR"
     gene            complement(2202..2342)
                     /locus_tag="VMZ53_06900"
     CDS             complement(2202..2342)
                     /locus_tag="VMZ53_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28216.1"
                     /translation="MSKKFTKTKKAASADAALTPEQKILERQRFLMQRTLLKRRRRKS
                     HQ"
     gene            2458..2790
                     /locus_tag="VMZ53_06905"
     CDS             2458..2790
                     /locus_tag="VMZ53_06905"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUS28217.1"
                     /translation="MFSALSDPTRRAILARLAKGEATVSELAEPFGLAQPTISKHLRI
                     LEEAGFIEQGRDAQRRPRSLVVGGPLKDIDSWLQPYREQWERRFDRLESFLEKKKSSK
                     RETNKGDK"
     gene            2790..3245
                     /locus_tag="VMZ53_06910"
     CDS             2790..3245
                     /locus_tag="VMZ53_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HUS28218.1"
                     /translation="MLTAEQTKTTIDHPTFTITFKRKLAATPDVVFDAWTKPDEISQW
                     WDPDGAPLRECAIDLRVGGEFKLVNNGHSPPFAGVYRVLERPSLIVFDAMGAVGTVRL
                     DGDQRGTQMTVTIRCANAEHLEHFVKLGVADGTERTLNNLVARIAAKTA"
     gene            complement(3249..6041)
                     /locus_tag="VMZ53_06915"
     CDS             complement(3249..6041)
                     /locus_tag="VMZ53_06915"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28219.1"
                     /translation="MAEGPDPEAATVDASSSGSSGPARVRPPAQPATLASGTKIDRYI
                     IERPLGAGGMGVVYAARDPELDRRVAIKLLHAGAPAERLRREAQALAKLHHPNVVTVF
                     DVGQHDGQSFVAMALVDGKNLRTWQRDARPVRESLRVILEAGRGLVAAHDAGLMHRDF
                     KPDNVFVSHHGNVLVGDFGLARSADDAPDASGSDLSAADTGLTMTGALVGTPAYMAPE
                     QARGEPEVASDQFAFCVTAWELLYGERPFAGSNVDEVMDAIEHGRIVEPKDDRGVPSR
                     VRRALERGMAARPADRWPTIDALLTELSGQRARRRRLAVLAAIAVIGCFAIAWGVSRW
                     TSSPASRCRPADERLAGTWNDATRKQLRAYLVAAAPFAETAIDNAMRGIDRYSGNWKR
                     AWVKTCSAKEISDTAFDLEIQCLDRRLTSLGITVGELRTADAKGASNVLESVELLPDI
                     TACGDATTLRKVSQAPAAKQPQIKELENRITRAETLTALGKWNEARDQFATLDHEVAA
                     LEFAPLAARAALAHAGVELTTERWPDAKRLLESAIAQSNASGDDATLGHAYVDLANVS
                     RIQGDVPHAKELAAIAKAVVHRIGDPRALTDNLALVDAELLAADGKLDDAITALGRLI
                     SASEPTDVRQRAKLEKRRADLLIIQGHVDEAAAAYDAALKDTAVAVGENHPLYGERLA
                     ERGQFTVLRGKVADGLADWDRAYKILVGTVGPDDPTTVETEEHLAGVDVWLGRYDQGL
                     ARERHVVEVRERLSGAEDTTRYRVEYTHLLLIAGKVADAEREIERSLEVARKTPRTPL
                     IVPDAERELAMVRLAQGRVDDAQKIFDDSLRTVSKVLASDNDALELWWVAGARISLAR
                     GNTDAAIQQASKVTRGIEIGEAKLVLAQALAKKGDRAGATKAIEESYAMLEGLMPGGN
                     ARPDLRAQLAKLKASL"
     gene            6179..7153
                     /locus_tag="VMZ53_06920"
     CDS             6179..7153
                     /locus_tag="VMZ53_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS28220.1"
                     /translation="MRLVAVLVLTLVASSVARADSDVAVVVSGPEGIATHTRDVVETW
                     FASNKLTINKSPLPKDALKTLLNCFVLADMSCARGVVEARAKASNIIGITEQVSGKKD
                     KRSVQLGAYWIAKEHEVVSVQRTCDACTDEVLEKMLISMLGDLAELAPTMAGTIHVTS
                     TPSGLKATIDGETDAKLKTPFDQKLSFGTHMISVMRDGRVVAQKKVDITPGADLDVPI
                     EVREEPKPKQTIKIVERRSSRAVPVVLLTVGVAACVTGGVLYKYGGPTGDAYTYRNMR
                     PAALATGISGGVAVVVGTVWLLRGGSKTSHPEVSITPTETTVGWARTF"
     gene            7153..8619
                     /locus_tag="VMZ53_06925"
     CDS             7153..8619
                     /locus_tag="VMZ53_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28221.1"
                     /translation="MKRASILLVLFAGVSACEQRDPLFCGMHMDDPRCLAGDGGLPEG
                     FVVIGGTVTGLTGSGLVLSNNGSDDKQVSSNGAFSFSTAIPQGSTYDVTVGTQPTNPS
                     EMCTVANGSGTANSDVADIEVTCMPAAYTVGGNVAGFTPGGNIVLTNNGGDDKTITAG
                     GAFTFATPVRSGQPYDVQIKSQQGETCSVVANTGVVGSGPITTIVVVCGSNLFTVGGS
                     PPQGGVTGLNGSVKLHNGNDTITVNANGAFAFPTPLTGGGSSMYNVTVTQQPAYPPAA
                     QNCTVTNGMGTVGTANITNVRVACMTRSFTVGGTASGLNGTVVLRNGGDMLSVSSNGS
                     FTFNNAVQSGFTYGVTVMTQPSGQQCTVSMGSGTVTNSNITNVSVTCINVGVKCGGSY
                     CNNGNICCDPGGSPSCRASNQCSAFELPCDDRADCSGGRLCCADRHNGNLSLQSVSCQ
                     SSCNNVNNPIQLCDPTKNECTSGSCQPWGQLSGYYACQ"
     gene            complement(8624..9502)
                     /locus_tag="VMZ53_06930"
     CDS             complement(8624..9502)
                     /locus_tag="VMZ53_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28222.1"
                     /translation="MRYLALLLLAGGCMVGDETDDVDTDEDFVDTTIGTLAAGGGSPG
                     VNGSHCVASPFNCRFHEGGSRVENAGGGEVWAVTPGASIRDGNGNALTTQTGDRLTFN
                     FGQTRSLAGKAHALALTTTNGSAGWYPFDHIVSEASFRDHVGNVDAKDPGDGRMGCYE
                     IRNSHDASIELKKVVYNSQVGADGHERAGDYLPLVRANGKRSANLIFSVPGFGLGGAT
                     SDHFPAGTKFRRVNVPTKTGKPSISIPLWTANGAGNYVVRSGSMRFLYGYIVGGGAKR
                     FGWMAQDALQVSTGCP"
     gene            complement(9644..10093)
                     /locus_tag="VMZ53_06935"
     CDS             complement(9644..10093)
                     /locus_tag="VMZ53_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28223.1"
                     /translation="MIEGTDPFFTVELTPVPRADLFSLRVVTFAPRAADQRWITEELE
                     GTRAIPYFVPTLRSVFDAIADEVGHRVIVIAYDALSREDIVDLRAHRARARGGTFIAL
                     GNIREHLRAPLRVTHVLPRPLGSEVLRGIIDGLDRQRDTAEIRKLDS"
     gene            complement(10277..10993)
                     /locus_tag="VMZ53_06940"
     CDS             complement(10277..10993)
                     /locus_tag="VMZ53_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28224.1"
                     /translation="MGETMKKLFVCLMLVGCGGDGAENVPIDNLGPELAAASCSKQFD
                     CCTDAEIMEQYMGITIDGQPITTEEQCVSFTNAFFTGFAVSAWKESIAQGRMEYDGDA
                     AGACVALINGVSCSEYANGPDLTEGDCRPFLIPKVANDGACSQDYECTSDNCVGAMTS
                     NGVKTDGMCKPMPGAGQPCDDNCADGYFCDYDQTANMDICQATKPIGADCFLDEDCAS
                     DYCDDVTDMCGMKPLTCDGR"
     gene            11067..12557
                     /locus_tag="VMZ53_06945"
     CDS             11067..12557
                     /locus_tag="VMZ53_06945"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HUS28225.1"
                     /translation="MSAHPHYDQLAAAFQRIYRFEHLQALAAWDHATYMPPGGNDARS
                     ASMAELAALIHRLRTAAELGTEIAAAQSEQLDDDRRANLREMQRIYRNATALPEDLVH
                     AASMAGAKCEHAWRKQRPENDWAGFAPNLREVVKLTRKQAEFLSQKLGMSKYDALMDR
                     FEPGMTSAEVDRLFGDVKSWLPALIQKVIDKQANEEVIAPVGPFPIATQRALCERVMK
                     LLSFDFHRGRLDVSTHPFSGGSPEDVRLTTRYRESEFLSSLMGTIHETGHGRYEQGRP
                     QEWLGQPMSEARSMAIHESQSLSFEMQLGRSRAFMELLAPLLVEYFGAQPAFEPENLF
                     RLYTRVKPGLIRVDADEVTYPAHVILRYEIERPLVEGEIEVDDIPALWDKKMMELLRQ
                     DTRGNYKDGPMQDIHWTDGAYGYFPCYTLGAMYASQWFATLRRAKPSVDADIARGDLG
                     GVFEWLEANVWRQASRFETDELCRRASGSRLDPKYLRAHLETRYLG"
     gene            complement(12559..14253)
                     /locus_tag="VMZ53_06950"
     CDS             complement(12559..14253)
                     /locus_tag="VMZ53_06950"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28226.1"
                     /translation="MKRPATIERASTVGQRAASLAETSPYRRTTASSITAETEPDEFG
                     PYLVYEKLGEGGMACVHRAEYVGSTGLRKQVALKRLHTDASEDPSLVDAFVHEAQLAA
                     KLHHPNIAQAYELGKIEDTYFIAMELVPGPTLLQIMNQSRSAGAVPLPIALELLVQIA
                     DALDHVHDLRDDAGNSLQLIHRDVTPANIIISRTGQAKLIDFGIAKARSARHATQAGY
                     IKGKHGYVAPEYTYGQLDYRADLFALGVVAHELLTGRRLFSTDSELDTLRNVRHMAIP
                     PPSRFRAGISPELDKIVLTALDRDPDKRWQSASAIRDAIVEETRRLKVAISGPQIRDW
                     VEWAFHQNARKDSSVERMLRGLSPSMSIEIAEIEVVRPSAPTVVVDPGTAADPPKPLH
                     ADDEDDNNADDEHVTVDARATTPYPLVDEPLSRDHLSDEPPTIDMSPSPSRPASPRRI
                     ERAASEVAFLPAVTMDSLLAKRAPTPAAAIAARAGTPAFVAKPVAAPARKAPAKRRAA
                     HPARGPHWRAPTRWMKPQARRSKVPAMILLALIAAAVLLVDQGLIDVDRWRQILGA"
     gene            14549..15319
                     /locus_tag="VMZ53_06955"
     CDS             14549..15319
                     /locus_tag="VMZ53_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HUS28227.1"
                     /translation="MSISKDTIVDALRLRYDAYSAETIFELACQRAELEGKTTFEGRD
                     LTAFRGALEKVGDRVGGVLARLDELAGNPPPPSQPAKQEAKPEPEQQKAKEPEQQKAK
                     DSDGKKGKGDKPSDAKPEAKSDAKTDAKPEAKSDKDAKTGGTEAKSADKSGGSDAKPV
                     ETTIVLRGLEVADGEQVMLCGELTDLGDWDPERARPMVRKGDEWLTTLELEPDTEVKF
                     KFLRRNAAGKVTWEAGDNRQVRANQRLDATWRTEAPAS"
     gene            15433..16050
                     /locus_tag="VMZ53_06960"
     CDS             15433..16050
                     /locus_tag="VMZ53_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28228.1"
                     /translation="MWRRLSGLLALSASGSLAFVIACGPPSRDVDGDRDASMGAADSD
                     DGAGSDGSGSGSGSGSGSGSGSSSGSGSGSGSDSTTIPGETVACYTQGNHDATCTLPT
                     HCCFTNYSSQHEGACQTTTCLFGTIDCDGPEDCTNGGHCCSTELFDSDGIAGYRIACQ
                     ASACETSVYVEELCHPTGPACANGGDCVSALGQNNDLPRALSICR"
     gene            16168..>16655
                     /locus_tag="VMZ53_06965"
     CDS             16168..>16655
                     /locus_tag="VMZ53_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28229.1"
                     /translation="MRKTVITIATLGGLAACATDTLDGDADRRKPRPGGVDAGISSSP
                     DAAPTTSDAGGGSSGGGSVSCYTEGAPSNTCSLPTHCCFTNYSAQHNGYCASESCTWG
                     TLSCDGPEDCASGQHCCSTQLRDSDGAIAGYRVACQANACGAPPTAEELCHPNSASCS
                     TG"
BASE COUNT         2840 a         5301 c         5702 g         2812 t
ORIGIN      
        1 gccgcccgcg cgcggtgcct tgacaccccc gccccagata cgtcattaat tcggtccctt
       61 cttccgaagt agctcagtgg tagagcaggc ggctgttaac cgccgggtcg ggggttcgaa
      121 accctccttc ggagccaacc agcatcggta gcaagaaccg gtctgccggt taactaccgg
      181 gcatccaccg gcgcaccgtc ggcggccagt agcttcagtg cggcgcgagg attgaccggc
      241 tatcgtggtt actacttgcg gccgtgatcc ttgccatgcc ccttgttacg gggctcgtcg
      301 ggagagggcg gagttgtcgc cggttccgat gctcgactcg ggccgggcac gcgcgacggc
      361 ggcgccgacg agctgttcgt gtacgccagc agggccatca ccgcgccgaa aatcccgaac
      421 accaccgtga tcgcgatccc gatgagccag cgacgttgcg cacccgcatc gcttcgtgcc
      481 gaggcactgt ctgtttgcgg atccatggga gtcgatcata cgcgcaacaa acacgccgcc
      541 gatgacaagg cgaccgaaga cctccaggcc gaatgcggat gtcgccgcgc acgagcacgt
      601 agcagcactt cccggccatc ctgccagctc agcgcttggc gcggagcccc cttcacgtgt
      661 tgttaacgtg agagcgttag gagctttcca tgattcgaaa atacacgggc aacaagaaga
      721 gcatcgaggc gcgcagcacc gacaacggtc gcacctggtc ggtgaagctc ttcgatacgg
      781 gacgcgtgac cgagtacacg ggcggaagcc tcgccgaggt tgatgcgctc gcggcgaagc
      841 acggcatgaa gctcgaccgc tagcgcgcgc ggcggcgatc ggcactattc gagcgccgcg
      901 tcgaggcggt tcagcgagag ccagttctcg atcagctcgc gcgcggactt cgtgcgcggc
      961 gtcggtacgg tgatgcggtg ctgccatgcg ccgtcgccgg tgagatcgaa gcggccccac
     1021 gagcggatcg cgccgagggt ctcgagctcg cgcaagaggc cgcgcgcaat gccgtcgtag
     1081 cgaccacgcg tatgttcgtc atcgcccgcg atcacgagtg tggtgcctgc gggaggatcg
     1141 tcggagatgt ccttgatgcg ttcgatcgcg tcggtgtcgc tcgcggcatc accgatggcg
     1201 atgacggccg cgcggacctc ggcgaacagt gtgagcgtgg agcggcggct cagctttgcg
     1261 acgtcgcggg tcggtggcgc gggtgccgcg aggaagaatg gctctgcttg gcccgcgcgg
     1321 acgccgtcga aaccaacccg cgagccgagc gcgagtgcag acagccagcg cagccacgca
     1381 ggtagcgcgg cgcccggcgc cacggtgcgg agctcggtga cgagcggcac cgcgaggagg
     1441 atcgcccagc cgaagctgcc gaaccaacca agcccgttat ggccgagggc gcgggcacga
     1501 acgaactgac gcacgtgcgg ccacgcgagc tggaacactt cgtcgcgcgc gttgtcgcgg
     1561 agctgagacg cgagcagcgc ggcgtcgcgc gggccggagc tcatccgccg cgagacgtca
     1621 tcgtcgccgg tgacgaccgt gacatcgacg ggcatgccgt cgagcgttcc gtgcaagcgc
     1681 ggcgggattg tggggaggag ctcgaagccg agggcggtcg cgagacgcgc gggattgtcg
     1741 ttcgtcacga ggacgtcgac gtcgcttgcg tggtccggcg cgtagatgct cgagccgtag
     1801 ccttcgaccg tggcgccggg agccgcgacg gcgcgcagtc gtgcgagcac acgcgacgcg
     1861 gcggaatcag gcacagcgta cggtagcagc gtgcgacagt cgccgcatgc acatgctgct
     1921 cgtgaagttc cacagcgggc tcgacgacaa aacggttgcg cggctactcg aagagcggct
     1981 gccgctgttc cgcgccgtgc ccggcctcgt ccagaagtat tacgcgcgcg aagaatcgag
     2041 cggcgactac gtcggctgct acctcttcga gtcgaagcag gcggtgctgg actatcgcgc
     2101 gtcggagctc gcgaagagca tcccgccgac gtacgccgtc gaaggtgtgg ctggtatcga
     2161 gatgctcgaa gtcctgttcc cgctgcgata gatgtggcgg gctactggtg cgacttgcgg
     2221 cggcggcgct tgagaagcgt gcgctgcatg agaaagcgct ggcgctcgag aattttctgc
     2281 tctggggtca gggcggcgtc agccgaagcg gccttctttg ttttcgtgaa ctttttgctc
     2341 atggggccgg caccgtaaca gaagtggcgg gcgacaggct cggtagacga ccttgacaac
     2401 attgccgtgt ggctatatag ctaagtggca atgaatagcg acgctcgatt gagcacggtg
     2461 ttttccgcac tctcggatcc gacgaggcgg gcgattctgg cgcgtcttgc gaagggcgag
     2521 gccacggtgt cggagcttgc cgagccattt ggcttggcgc agccgaccat ttcgaagcac
     2581 ctgcgcattc tcgaagaggc ggggttcatc gagcaggggc gcgatgcgca gcgcaggccg
     2641 cgcagcctcg tcgtcggtgg gccgttgaag gacatcgatt cctggctgca gccgtaccgc
     2701 gagcagtggg agcgccgctt tgaccgcctc gagtcgtttc tcgagaagaa gaagtcgtcc
     2761 aagcgcgaga ccaacaaggg agacaagtga tgctgaccgc agaacagacc aagaccacga
     2821 tcgatcaccc gacatttacg atcacgttca agcgcaagct tgctgcgacg cccgacgttg
     2881 tgttcgacgc ctggacgaag cccgacgaga tcagccagtg gtgggatcca gacggcgcgc
     2941 cgctgcgcga gtgcgcgatc gatttgcgcg tcggcggcga gttcaagctc gtcaataatg
     3001 ggcacagccc gccgttcgcc ggcgtgtatc gcgtgctcga gcggccgtcg ctgatcgtgt
     3061 tcgatgcgat gggagcggtg ggcaccgtac gcctcgatgg cgatcagcgt ggcacgcaga
     3121 tgacggtcac gatccggtgc gcgaatgcgg agcacctcga gcacttcgtg aagttgggcg
     3181 tcgccgacgg gacggagcgc acgctgaaca acctcgtcgc gcggatcgca gcgaagactg
     3241 cgtagcagct ataacgacgc tttcagcttc gcgagctgcg cgcgtagatc agggcgcgcg
     3301 ttgccgccgg gcatcaggcc ttcgagcatc gcgtacgact cttcgatcgc tttggtcgcg
     3361 ccggcgcggt cgcctttttt tgcgagcgct tgcgcgagca cgagcttcgc ttcgccgatc
     3421 tcgatgccgc gagtgacttt ggatgcttgc tggatcgcag catcggtgtt gccgcgagcg
     3481 agcgagatcc gcgcgcctgc gacccaccag agctcgagcg cgtcgttgtc gcttgcgagg
     3541 accttcgaga cggtgcgcag gctgtcgtcg aagatcttct gtgcgtcgtc gacgcgacct
     3601 tgagcgaggc gcaccatcgc gagctcgcgt tcggcatcgg ggacgatgag cggcgtgcgc
     3661 ggcgtcttgc gtgcgacctc gaggcttcgt tcgatttcgc gttcggcgtc ggcgaccttg
     3721 ccggcgatga ggagcaagtg ggtgtactcg acgcggtagc gcgtggtgtc ttcggcgccg
     3781 gacaggcgct cgcgcacttc gacgacgtgg cgttcgcgag cgaggccttg gtcatagcga
     3841 ccgagccaca cgtcgacgcc cgcgagatgc tcctctgtct cgacggtcgt tggatcgtcg
     3901 gggccgacgg tcccgacgag gatcttgtac gcgcgatccc agtcggccag cccgtcggca
     3961 accttgccgc gcaacacggt gaattggccg cgctccgcga ggcgttcgcc gtacagcgga
     4021 tggttctcgc cgacggcaac ggctgtgtct ttgagggcgg catcgtaggc cgccgcggct
     4081 tcgtctacgt gaccttggat gatgagcagg tcagcgcggc gcttctccag cttggcgcgc
     4141 tgacggacgt ccgtcggttc gctcgcggag atgaggcggc cgagcgccgt gatcgcgtcg
     4201 tcgagcttgc cgtcggcagc gagtagctcg gcgtcgacga gcgcgaggtt gtccgtgagt
     4261 gcgcgcgggt caccgatgcg atgcacgacc gcctttgcga tcgccgcgag ctccttcgca
     4321 tgggggacgt cgccttgaat gcgcgagacg ttcgcgaggt caacgtaggc gtggcctagt
     4381 gtcgcgtcgt cgccgctcgc gttggattgc gcgatcgcgc tctcgaggag tcgcttggcg
     4441 tcgggccagc gctcggtcgt gagctcgacg cccgcgtgag cgagtgcggc acgcgcggcg
     4501 agaggtgcga actcgagagc ggcgacctcg tgatcgagcg tggcgaactg gtcgcgcgct
     4561 tcgttccatt taccgagcgc ggtgagagtc tccgcacgcg tgatgcgatt ttcgagctct
     4621 ttgatttgcg gctgcttggc ggctggcgcc tgggacacct tgcgcagcgt cgtggcgtcg
     4681 ccgcacgcgg tgatgtcggg aagcagctcg acgctctcga ggacattgct ggcgcccttc
     4741 gcatccgccg ttcgcagctc accgactgtg atgcccaacg acgtaaggcg gcgatcgagg
     4801 cactggatct cgagatcgaa tgccgtgtcg gagatttctt tcgccgagca ggtcttcacc
     4861 cacgcgcgct tccagttgcc gctatagcga tcgatgcccc tcatcgcgtt gtcgatcgcg
     4921 gtctccgcaa acggcgccgc cgcgacgagg tacgcgcgca gctgctttcg tgtcgcatcg
     4981 ttccacgtac ccgccagtcg ctcgtccgcc ggacgacacc tcgacgccgg gctgctcgtc
     5041 catcgcgata ctccccatgc gatcgcgaaa cagccaatca ccgcgatggc ggccaacacg
     5101 gcgagacgcc ggcggcgagc gcgttgccct gacagctccg tcaacagcgc gtcgatcgtc
     5161 ggccatcggt cggccggccg cgcagccatt ccacgctcga gcgcgcgacg cacgcgagac
     5221 ggcacaccgc gatcgtcctt cggctcgacg atgcgcccgt gctcgatggc atccatcact
     5281 tcatcgacgt tgctgcccgc aaacggacgc tcgccgtaca gcagctccca cgcggtcacg
     5341 cagaatgcga actggtccga tgcgacctcg ggctccccgc gcgcttgctc cggcgccatg
     5401 tacgcgggcg ttccgacgag agcgccggtc atcgtcagtc ccgtatccgc agcggagaga
     5461 tcgctgcccg atgcatcggg cgcatcgtcg gcagaccgcg cgagaccaaa gtcgccgacg
     5521 agcacgttgc cgtgatgcga gacgaacacg ttgtcgggct tgaagtcgcg atgcatcagg
     5581 ccagcgtcgt gcgccgcgac gagaccgcga cctgcttcga ggattacgcg gagcgactcg
     5641 cgaaccgggc gagcgtcacg ctgccacgtg cgcaggttct tgccgtcgac gagcgccatc
     5701 gcgacgaacg actgaccatc gtgttggccg acgtcgaaga cggtgacgac gtttggatgg
     5761 tgcagcttcg cgagcgcttg cgcttcacgg cgaaggcgtt cggcaggagc gcccgcatgc
     5821 agcagcttga tcgctacacg gcgatcgagc tcgggatccc gagcggcgta gacaacgccc
     5881 atcccgccgg cgccgagcgg gcgctcgatg atgtaacgat cgatcttcgt tccgctcgct
     5941 agcgtcgccg gttgcgcggg aggccggacg cgggccggac cgctgctgcc actcgagctg
     6001 gcatcaaccg tcgccgcctc aggatccgga ccctccgcca tgtggacgtc gatgctactg
     6061 cgggcatcgc tgagaatgta acagtcgcca catgcgcaaa ccgcgcacgt aagggtgctt
     6121 tgcgcttagg gtttgcggag tgacgggagg cagcgcgaga cgggatacaa taggtgccgt
     6181 gaggctggtt gcggtactcg tgcttacgct cgtggcgagc tctgtcgcgc gcgcggacag
     6241 cgacgttgcg gtcgtcgtca gcgggcccga gggcatcgcg acgcacaccc gcgacgtggt
     6301 cgagacgtgg ttcgcgtcga acaagctgac gatcaataaa tcgccgcttc cgaaggatgc
     6361 gctgaagacg ctcctcaact gcttcgtcct cgcagacatg tcgtgtgcgc gtggcgtggt
     6421 cgaagcgcgc gcgaaggcga gcaacatcat cggtatcacc gagcaggtct cgggcaagaa
     6481 ggacaagcgc tcggtgcagc tcggcgcgta ttggatcgcg aaagagcacg aggtcgtgtc
     6541 tgtgcagcgc acgtgcgacg cgtgcaccga tgaggtgctc gagaagatgc tgatctcgat
     6601 gctcggtgat ctcgccgagc tcgcgccgac gatggcgggc accattcacg tgacgtcgac
     6661 gccgtcggga ctcaaggcga cgatcgacgg cgagacggac gcgaagctca agacgccgtt
     6721 cgatcaaaag ctcagcttcg gcacgcatat gatctcggtc atgcgcgatg gccgtgtcgt
     6781 cgcgcagaag aaggtcgaca tcaccccggg cgccgatctc gacgtgccga tcgaagttcg
     6841 cgaggagccc aagccgaagc agacgatcaa gatcgtcgag cgacgctcgt cgcgcgcggt
     6901 gcccgtcgtg ctcttgaccg tcggcgtcgc agcctgcgtg acgggcggcg tgctctacaa
     6961 atacggcggc cccacgggcg acgcatacac gtatcgcaac atgcggcccg cggcgctcgc
     7021 gactggcatc agcggcggtg tggcggtcgt cgtcggaacc gtctggctcc tgcgtggcgg
     7081 tagcaagacc tcgcatcccg aggtttcgat cacgccgacg gagacgaccg tcggatgggc
     7141 aaggacgttt tgatgaagcg cgcatccatt cttctcgtgt tgtttgcggg cgtgagtgcg
     7201 tgcgagcagc gcgatccgct gttctgcggg atgcacatgg atgatccgcg ctgtctcgcg
     7261 ggcgatggcg gccttccgga aggcttcgtc gtcatcggcg gaaccgtgac cggtttgacc
     7321 ggcagcggtc tcgtgctcag caacaacggc agcgacgaca agcaggtctc gtcgaacggc
     7381 gcgttctcgt tctcgacggc aattccgcag ggctcgacgt acgacgtcac cgttggcacg
     7441 cagccgacga acccgagcga gatgtgcacg gtcgcgaatg gctcgggaac ggcgaacagc
     7501 gacgtcgccg acatcgaagt gacctgcatg ccagcggcgt acaccgtcgg tggcaacgtc
     7561 gcgggcttca cgcccggcgg caacatcgtg ctgaccaaca acggcggcga cgacaagacg
     7621 atcaccgcgg gcggcgcctt cacgttcgcc acgccggtgc gaagcggtca gccgtacgac
     7681 gtgcagatca agagccagca gggcgagacc tgcagcgtcg tcgcgaacac cggcgtcgtc
     7741 ggcagcggac cgatcaccac gatcgtcgtc gtgtgcggct caaatctatt tacggtcggt
     7801 ggctcgcccc cgcaaggcgg cgtgacgggc ctcaacggct cggtgaagct ccacaacggc
     7861 aacgacacga tcaccgttaa cgccaacggt gccttcgcat tcccgacgcc gctcacgggc
     7921 ggtggcagct cgatgtacaa cgtgacggtc acccagcagc cggcgtatcc gcccgcggcg
     7981 cagaactgca cggtcacgaa cggcatgggc accgtcggaa ccgcgaacat cacgaacgtt
     8041 cgcgtcgcct gcatgactcg cagcttcacc gtcggcggga ctgcgagcgg gctgaacggc
     8101 acggtcgttc tcaggaacgg tggcgacatg ctgtcggtga gctcgaacgg atcgttcacg
     8161 ttcaacaacg ccgtgcagag cggcttcacg tacggcgtca ccgtgatgac ccagccgagc
     8221 ggtcagcagt gcaccgtctc gatgggatcc ggaacggtga cgaactcgaa catcacgaac
     8281 gtctcggtga cgtgcatcaa cgtcggcgtc aagtgcggcg gtagctactg caacaacggc
     8341 aacatctgct gcgatccggg tggcagccca tcgtgtcgcg cgagcaacca atgcagcgca
     8401 ttcgagcttc cgtgtgacga ccgcgcggac tgcagcggtg ggcgcctctg ctgcgcggat
     8461 cgtcacaacg gcaacctgtc gttgcagagc gtctcgtgcc agtcgagctg caacaacgtg
     8521 aacaatccga tccagctgtg cgatccgacg aagaacgagt gcacgagcgg cagctgccag
     8581 ccgtggggcc agctgagcgg ctactacgcg tgtcagtagc ggctcagggg cagcccgtcg
     8641 agacctgcag cgcgtcctgc gccatccagc cgaagcgctt cgcgccgccg ccgacgatgt
     8701 agccgtagag gaagcgcatc gagcccgagc gcacgacgta gttgccggct ccgttcgctg
     8761 tccagagtgg aatcgagatc gacggcttgc ccgtcttcgt cgggacgttc acgcgccgga
     8821 acttcgtgcc ggccgggaag tgatcgctcg tcgcgccgcc caggccaaag cccggcacgc
     8881 tgaagatgag gttcgcgctg cgcttgccgt tcgcgcggac gagcgggagg tagtcgcccg
     8941 cgcgctcgtg gccgtcggcg cccacctggc tgttgtagac caccttcttg agctcgatcg
     9001 atgcgtcatg cgaattgcga atctcgtagc agcccatgcg gccgtcgccc ggatcctttg
     9061 catcgacgtt gccgacgtgg tcgcgaaatg atgcttcgct gacgatgtga tcgaacggat
     9121 accagcctgc gctgccgttc gtcgtcgtga gggcgagcgc gtgcgccttg cccgcgagcg
     9181 aacgcgtttg gccgaagttg aaggtcaggc gatcgcccgt ctgcgtggtg agcgcgttgc
     9241 cgttgccgtc gcgaatcgag gcccccggtg tgaccgccca aacttcgccg ccgcccgcgt
     9301 tctctacgcg cgagccgcct tcgtgaaagc ggcagttgaa cgggctcgcg acgcagtggc
     9361 taccgttgac gccgggcgaa ccgccacccg ccgcaagcgt accgatcgtc gtgtcgacga
     9421 agtcctcgtc ggtatctacg tcgtcggtct cgtcaccgac catgcaaccg ccggcgagga
     9481 gtagcaaagc gagatatctc atgcgtgctg ccccgtttgc agaaggcatg cctcgcgtgg
     9541 tcacgcgagt tgaacgcgct ttgtcgcgta agcgtgcgat cgctcgcgcg tggtgcgctg
     9601 cgccgtgccc acgcctgtct gcagcggtgg ggccggaaag cggctacgag tcgagcttgc
     9661 ggatctcggc ggtatcgcgt tggcgatcga ggccgtcgat gatgccgcgg aggacttcgc
     9721 tgccgagcgg ccgcggtagg acgtgggtca cgcgaagcgg agcgcgaagg tgctcgcgaa
     9781 tgttgccgag cgcgatgaac gtgccgccac gagcccgcgc gcggtgagcg cgcaggtcga
     9841 cgatgtcttc tcgcgagagc gcgtcgtacg cgatgacgat cacgcgatgc ccgacttcat
     9901 ccgcgatcgc gtcgaacacg gagcgcagcg tgggaacgaa gtacgggatg gcgcgcgtgc
     9961 cctcgagctc ctcggtgatc cagcgctgat cggcggctct cggcgcgaac gtgacgacgc
    10021 gcagcgagaa cagatccgcg cgaggcacgg gtgtcagctc gaccgtaaaa aacgggtcgg
    10081 ttccctcgat catcgtcgag accattgtgc tcgatcaccg acgaggaacc ggggtattcg
    10141 acgggtaccc gggccgcgcc gatgtcagct tggtagactg catcaccatt gttgcgcgac
    10201 gctttgcgcg ggctgcgaag atcgacggtg atgaggccga tagctctgta gttgcgtgtg
    10261 ggtttgcttc ggtcgcctag cggccgtcac acgtcagcgg cttcatcccg cacatatcgg
    10321 tgacgtcgtc gcagtaatcg ctcgcgcagt cctcgtcgag gaaacagtcg gcgccaatgg
    10381 gcttcgtcgc ctggcagatg tccatgttcg cggtttggtc gtagtcacag aaatagccat
    10441 cggcgcagtt gtcgtcgcat ggctggcccg cgcccggcat cggcttgcac atgccgtcgg
    10501 tcttcacgcc gttcgacgtc atcgcgccga cgcagttgtc gctggtgcac tcgtagtctt
    10561 gcgagcacgc gccgtcgttc gcgactttcg ggatcaggaa cggcctgcag tcgccctcgg
    10621 tgagatcggg gccgttcgcg tactcggagc acgacacgcc gttgatcagc gcgacgcatg
    10681 cgcctgctgc atcaccgtcg tattccatgc ggccttgcgc gatcgattct ttccacgcac
    10741 tcacggcgaa accggtgaag aacgcgttcg tgaacgacac gcactgttct tccgtcgtga
    10801 tcggctggcc atcgatcgtg atgcccatgt actgctccat gatttcggca tcggtgcagc
    10861 agtcgaactg cttgctacac gacgctgcgg cgagctccgg gccgagattg tcgatcggga
    10921 cgttctcggc gccgtcacca ccgcagccaa cgagcattag acagacgaag agcttcttca
    10981 tcgtttcccc cacgtcgatc gtacggcagg tgatcagccg ccgtcactgc ctcacgtttc
    11041 catcacgcgt gcggtagggt aggcgcatga gcgcgcatcc tcactacgat cagcttgcgg
    11101 cggcatttca gcgcatctat cgcttcgagc atttgcaggc gctcgccgcg tgggaccacg
    11161 ccacgtacat gccgcccggt ggcaatgacg cccgatcggc atcgatggca gagcttgccg
    11221 ctttgatcca tcgacttcgc acagcggccg agctcggcac cgagatcgct gcggcgcaga
    11281 gcgagcagct cgacgacgat cgtcgcgcaa acctgcgcga gatgcagcgc atctaccgca
    11341 atgcgacggc gctgccggaa gacctcgtgc atgccgcttc gatggcaggc gcgaagtgcg
    11401 agcacgcgtg gcgcaagcag cggcccgaga acgactgggc ggggttcgcg ccgaacctgc
    11461 gcgaggtcgt gaagctgacg cgcaaacagg cggagtttct gtcgcagaag ctcggcatgt
    11521 cgaaatacga cgcgctgatg gatcgcttcg agccgggcat gacgagtgcc gaagtcgatc
    11581 ggctgttcgg cgatgtgaag tcgtggctgc cggctttgat ccagaaggtg atcgacaagc
    11641 aggcaaacga agaagtcatc gcgccggtag ggccgttccc gatcgcgacg cagcgcgccc
    11701 tctgcgagcg cgtgatgaag ctgctcagct tcgacttcca tcgcggccgg ctcgatgtca
    11761 gcacgcatcc gttctccggc ggctcgcccg aggacgttcg gctgacgacg cggtatcgcg
    11821 agagcgagtt cctctcgagt ctcatgggaa cgatccacga gaccggtcac ggtcggtacg
    11881 agcagggcag gccgcaggaa tggctcggcc agccgatgtc ggaagcccgc tcgatggcga
    11941 tccacgagag ccagagcttg tcgttcgaga tgcagctcgg tcgcagtcgc gcgttcatgg
    12001 agctcctcgc gccgttgctc gtcgagtact tcggcgcgca gccggcgttc gagccggaga
    12061 atctgttccg gctgtacacg cgcgtgaagc ccggcctcat tcgcgtcgac gccgacgagg
    12121 tcacgtatcc cgcgcacgtc atcctgcgct acgagatcga gcggcctctc gtcgagggcg
    12181 agatcgaggt cgacgacatt ccggcgctct gggacaagaa gatgatggag ctgctgcggc
    12241 aagacacgcg cggcaactac aaggatggtc cgatgcagga catccactgg accgatggcg
    12301 cgtacggcta cttcccctgc tacacgctcg gtgccatgta cgcatcacag tggttcgcga
    12361 cgctgcgccg cgcgaagccg tcggtcgatg cggacatcgc gcgtggcgac ctcggcggcg
    12421 tgttcgagtg gctcgaagca aatgtctggc ggcaagcgtc tcgcttcgag actgacgagc
    12481 tgtgccggcg tgcgagcggc tcgcgtctcg atccgaagta tctgcgcgcg catctcgaga
    12541 cgcgctacct cggctgagct acgcgccgag aatctggcgc cagcggtcga cgtcgatgag
    12601 cccctgatcg acgaggagca ctgctgcggc gatgagcgcg agcaggatca tcgcgggcac
    12661 cttcgagcga cgcgcctgcg gcttcatcca gcgcgtcggt gcgcgccagt gcgggcctcg
    12721 cgcgggatgg gcggctcgcc gtttcgccgg cgctttgcgc gcgggcgcgg caacgggctt
    12781 ggcgacgaat gccggtgtcc ctgcgcgggc ggcaatcgcc gcggcgggcg tcggcgcgcg
    12841 tttcgcgagc aacgagtcca tcgtgacggc gggcaggaag gcgacctcgg acgcagcgcg
    12901 ctcgattcgg cgcggtgaag cgggccgcga tggcgatggc gacatgtcga tcgtcggcgg
    12961 ctcgtcggag aggtggtcgc gcgagagagg ctcgtcgacg agcgggtacg gtgtcgtcgc
    13021 gcgcgcgtcg acggtcacat gctcgtcgtc agcgttgttg tcgtcctcgt cgtcggcgtg
    13081 gagcggtttc ggtgggtccg ccgcggtgcc ggggtcgacg acgaccgtgg gtgcgctcgg
    13141 ccgcacgacc tcgatctcgg cgatctcgat ggacatgctc ggcgagagac cgcgcagcat
    13201 gcgctcgacg ctcgagtctt tgcgcgcgtt ttggtgaaac gcccactcga cccagtcgcg
    13261 aatctgcggg ccgctaatcg ccactttgag ccgtcgcgtc tcctcgacga tcgcatcgcg
    13321 gattgcactc gcactctgcc agcgtttgtc gggatcgcga tcgagcgcgg tcagcacgat
    13381 cttgtcgagc tcgggcgaga tgcccgcgcg gaatcgcgat ggcggcggga ttgccatgtg
    13441 gcgaacgttg cgaagcgtgt cgagctcgct gtccgtcgag aacaggcggc ggccggtcag
    13501 gagctcgtgc gcgacgacgc cgagcgcgaa tagatcggcg cgatagtcga gctggccgta
    13561 cgtgtactcg ggcgcgacgt agccgtgctt gcccttgatg taacccgctt gcgtcgcgtg
    13621 acgtgcgctg cgcgctttcg cgatgccgaa gtcgatcagc ttcgcttggc ccgtccgcga
    13681 gatgatgatg ttcgccggcg tgacgtcgcg gtggatgagc tgcagcgagt tgcccgcgtc
    13741 gtcgcgaagg tcgtgcacgt gatcgagcgc gtcggcgatc tggacgagca gctcgagcgc
    13801 gatcggcaac ggcaccgcgc ctgcgctgcg gctctgattc atgatctgga gcagcgtcgg
    13861 tccgggaacg agctccatcg cgatgaagta cgtgtcctcg atcttgccga gctcgtatgc
    13921 ctgcgcgatg ttgggatggt gcagcttcgc ggcgagctgc gcttcgtgca cgaacgcatc
    13981 gacgagcgac gggtcctcgg aagcatcggt gtgcaggcgc ttgagggcga cctgcttgcg
    14041 aagccccgtc gacccgacgt actccgcgcg atggacgcac gccatgccgc cttcgccgag
    14101 cttctcgtac acgaggtacg ggccgaactc gtcgggctcg gtctccgcgg tgatcgacga
    14161 cgcggtcgtc cgccgatacg gcgaggtctc cgcgagagac gctgcgcgtt ggccgaccgt
    14221 cgacgcgcgc tcgatcgtgg caggtcgctt catgcgacgc acgcgtgcca cgggagatgc
    14281 catgtgctca cgatgacaaa ccgcgcgccc gacaacaacg aacgagtttg tcgtcggaaa
    14341 cgcgagcttt gtcagtacct gttggtgcgc taaacccgcg atggccggtg gcgcgaccaa
    14401 cgcgcgatcg ggggtgggac tcttgtccgt gcgagcggct acagtcgttg catccggcga
    14461 ctttgcgctt cgggcgcgag gtatcgcttg cacgaacgcc cccgctgcgc tcgcatcgac
    14521 ggcggggtgc atgtagcctt gcgccgcaat gtcgatcagc aaggacacta tcgtcgacgc
    14581 gcttcgcctg cgctacgacg cctactccgc cgaaacgatc ttcgagctcg catgtcaacg
    14641 cgccgagctc gagggcaaga cgacattcga gggtcgcgac ctcaccgcat tccgcggcgc
    14701 actcgagaag gtgggcgatc gcgtaggtgg cgtgctcgcg cgcctcgacg agctggctgg
    14761 caacccgccg ccgccctcgc agcccgccaa gcaagaggca aagccggagc ccgagcagca
    14821 gaaggccaag gagcccgagc agcagaaggc gaaggactcc gacggcaaga agggcaaagg
    14881 cgataagccc tccgacgcga agcccgaggc aaagtccgac gcgaagaccg acgcgaagcc
    14941 cgaggcgaag tccgacaagg acgcgaagac cggcggcacc gaggcgaagt ccgcggacaa
    15001 gagcggcggc agcgacgcga aacctgtcga gacgacgatc gtgctgcgcg gtctcgaggt
    15061 cgcagacggc gagcaggtca tgctgtgcgg cgagctcacc gacctgggcg actgggatcc
    15121 agagcgcgcg cggccgatgg tccgcaaagg cgacgagtgg ctgaccacgc tcgagctcga
    15181 gcccgacacc gaggtgaagt tcaagttcct gcgccgcaat gcggctggca aggtcacgtg
    15241 ggaagcgggc gacaaccggc aagtgcgcgc gaaccagcgc ctcgacgcga cctggcgtac
    15301 cgaagctccc gcgtcgtaac ctccgtcgac gcgacctggc gtaccgaagc tcccgtgtcg
    15361 taaccgtcga cgacggcagc gccgaaaatc gtgcgtgatc tcgtcgatga gctgcgttag
    15421 aaccgggccc gcatgtggcg tcggttgtcc gggctgctcg cgctaagcgc atcgggatcg
    15481 ctcgcgttcg tgatcgcgtg cggacctccg tcgcgcgatg tcgatgggga cagggatgcg
    15541 tcgatgggcg cggccgacag cgacgatgga gcgggaagcg acggcagcgg cagcggaagc
    15601 ggcagcggaa gcggaagcgg cagcggcagc agtagcggaa gcggcagcgg aagcggatct
    15661 gactcgacga ccattccggg cgaaaccgtc gcttgctaca cgcaaggcaa ccacgacgcg
    15721 acctgcaccc tccccaccca ctgctgcttc acgaactaca gctcgcagca cgagggcgct
    15781 tgccagacga caacatgcct gttcggcacg atcgattgcg acggtcccga ggactgtacg
    15841 aacggcggac attgctgctc gaccgagctg ttcgacagcg acggcatcgc cggctatcgc
    15901 atcgcgtgtc aggcatcggc gtgcgagacc tcggtctacg tcgaggagct ctgccatccg
    15961 acgggcccgg cctgcgcgaa cggcggcgac tgcgtgtcgg cgcttggcca gaacaacgat
    16021 ctcccgcgcg cgctctcgat ctgtcgctga gtcgcgagcg gggcccggcg ttacatcgct
    16081 cgcgaccgcc tcaccggcga gcgtactttc gcttccctca cgtaaggggt ccgcggtatg
    16141 cactgaaccc gctccatgac cccaaccatg cggaagaccg tgatcacgat cgccacgctc
    16201 ggcggtctcg ccgcgtgcgc caccgacacg ctcgatggcg acgcggatcg ccgcaaacca
    16261 cgccccgggg gagtcgacgc cggtatcagc tcgagccccg acgctgcccc gacgacatca
    16321 gacgccggcg gcggttcatc cggcggcggc agcgtgagct gctacacgga gggcgcgccg
    16381 agcaatacgt gctcgttgcc cacgcactgc tgcttcacca actacagcgc tcagcacaac
    16441 ggctactgcg cgtccgagag ctgcacgtgg ggcacgctca gctgtgatgg tccggaagac
    16501 tgcgcgagcg ggcagcactg ctgctcgacc cagctgcgcg acagcgacgg cgccatcgcg
    16561 ggctaccgcg tggcatgtca ggcgaacgcg tgcggcgcac cgccaaccgc cgaagagctc
    16621 tgccatccca attcggcgag ctgctcgacg ggtga
//
LOCUS       DATLBZ010000129         4839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_128067, whole genome shotgun sequence.
ACCESSION   DATLBZ010000129 DATLBZ010000000
VERSION     DATLBZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128067"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VMZ53_06970"
     CDS             complement(<1..767)
                     /locus_tag="VMZ53_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HUS28230.1"
                     /translation="MRLRLHRWARRAMWLVTAINALWLALVIAVYAVPMPKRELSWSV
                     SVDYRDGEPAFVFLSPDEKWRLPVEMERVDPKFVEALVALEDKRFWSHSGVDPIAIVR
                     ATWSDVTKMRRVSGGSTLSMQLARLLEPRRRTIPNKLVDMFRALQLDARMSKQQILAM
                     YLSRTPYGKNVEGIESASRAYFGHSAQHLSPLEISTLLAVPQGPARFAPSKTNAERLR
                     ERRDTILRKLIAAGVFSQADATAALAEAKDAAPPTKLR"
     gene            complement(776..>4839)
                     /locus_tag="VMZ53_06975"
     CDS             complement(776..>4839)
                     /locus_tag="VMZ53_06975"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HUS28231.1"
                     /translation="TLQTRPDDDIDESEPFALIARRGDDLTYIRYKDLRADVTESSTS
                     GVPYIASTPYRASIFSDRGVYRPGDNAHVVAIVRDQKDKAPDQALPVEVKLVDPRARV
                     AKRITLKTNAAGVLAFDYDLPAFADTGHWRVTLSVADKPLSSYDLQVEEFVPERMKVA
                     IEPKAADALIGGKMAFDINARYLFGGSAVDSGVELSCQIEPSTFVPEENADLTYGVEP
                     KGKAVQLGEAREQLDPKGGITMECPAPDEDTTFTQTGELSATASVLEAGSGRATVKTA
                     SMMVHPEKFYIGVRTKAIRAEAGEPFTVEGMVVDWNGKLAPNATKQLQIDMIHLESEY
                     GYGYDDESGDSRYDRWLREVPEGKQTVKVENGKFTFDVTPGEAYGGYLVRVKAGKAKT
                     ELVLDGTYSYDYYEYGDGSRYDQTPRPAKPTQLKLDIPSDIKVGETVTAKLKSPYKGK
                     VLWTVETDHVVTAEWKDVSSAESSWTFKLDAFAPNVYVSAFLIKDPHAESRDAFMPDR
                     AYGVKSVHVVPTEFTQTVKVETPKEIRSSASLDVKLDLGPTPASTFAVVAVVDEGILS
                     LTNYKTPDPLAQLFAKRALGVETYETIGWTMLHQPAGTSSKTGGGDYEGEEEAGGGPL
                     DAGRVQPVKPVALWSGVLAVPSDGKLTVPFHVPTYRGQLRVMVITAGPQKIGRAETQV
                     TVKDPLVLQVTFPRFVTQNDQMEIPVFMTNMSGAPLEVTVKLDSAAIAIAGLAQPKTA
                     AAPLSFGGKDTGSIKIDNGRAETLVFQTTAKLPAGAAKLHVTATAKGSAGTFTVSDEV
                     DIPFMPAGPKDRVLQKIQVKAGKLDLSSQAALKGWVPLSEQTTFWLTSNPYGEAFDHL
                     KYLIHYPYGCIEQTTSSTRPLLYIGAIVEQVDPETSTKKVEDMVLAGVNRIFTMETPE
                     GGFGYWPGSTHPEDWGTAYATHMLLDAKKAGYAVPQDRLDNVLEWIEKRVGEYESNLR
                     DQTPYWYYNEQAESYMHFVLARAGKGKKARILKLVEHITEQQAKLPLTYRQNEMAEDL
                     YVLKAALYLAGDRRYEKELKTVDASPIRADRHNYWSFYSDRRRRAMQLSMFHDLFGND
                     PAGEPLANRVAESLSGQPSYYYNTQELVWGVTGLGKWVVGMGTKGVADGTLTADGTVI
                     QPRKTKQKTNDKTWSLQRASEYKSLTIDIPQSADGLWLVVNSSGVRQGADYKVGGNGM
                     SVTRTYRNLAGDEVDLTDGSVKLGDLVFVEVEVGNTSGEVIQNIALVDRLPAGFEVEN
                     PRLGRGFKADWVNPDDQWGVDYLNMRDDRIEAFGALSPSEKKTIVYTVRAVTSGKYAM
                     PPVEAEAMYDPTLWAREKGGTAVVGGPWTGKLL"
BASE COUNT          748 a         1613 c         1546 g          932 t
ORIGIN      
        1 cgcagcttcg tcggcggcgc agcgtctttc gcttcggcga gcgctgccgt ggcatccgcc
       61 tgcgagaaca cgccggctgc aatgagcttg cggaggatcg tgtcgcggcg ttcgcgcagc
      121 cgctccgcat ttgtcttcga cggcgcgaag cgcgcggggc cttgtggcac ggcaagcagc
      181 gtcgagatct cgagcggcga gagatgctgc gcggagtggc cgaagtacgc gcgcgacgcg
      241 ctctcgattc cctcgacgtt ctttccgtac ggcgtgcgcg acaagtacat cgcgaggatc
      301 tgctgcttgc tcatgcgcgc gtcgagctgc agcgcgcgaa acatatcgac gagcttgttc
      361 gggatcgtgc ggcgtcgcgg ctcgagcaac cgcgccagct gcatcgacag cgtcgagccg
      421 ccagacacgc gccgcatctt cgtcacatcg ctccacgtcg cgcgaacgat cgcgatcgga
      481 tcgacgcccg agtgactcca gaaccgcttg tcttcgagcg cgacgagagc ctcgacgaat
      541 ttcggatcga cgcgctccat ctcgaccggc agtcgccact tctcgtcggg cgagaggaag
      601 acgaaggccg gctcgccatc gcggtagtcg accgacaccg accacgacag ctcgcgcttc
      661 ggcatcggca ccgcgtagac cgcgatgacg agcgccagcc acagcgcgtt gatcgccgtg
      721 accagccaca tcgcgcgccg cgcccaccga tgcaggcgca gtcgcacggc cgcagctaca
      781 ggagcttgcc cgtccatgga ccgccgacca ctgccgtgcc gcccttttcg cgcgcccaca
      841 gcgtcgggtc gtacatcgct tcggcctcga cgggcggcat ggcgtacttg cccgacgtca
      901 cggcgcgcac cgtgtagacg atcgtcttct tctcgctcgg cgagagggcg ccgaacgctt
      961 cgatccgatc gtcgcgcatg ttcaagtagt caacgcccca ctggtcgtcg ggattcaccc
     1021 aatcggcctt gaagccgcga ccgagccgcg ggttctcgac ctcgaagcca gcgggtaggc
     1081 gatcgacgag cgcgatgttc tggatcacct cgccgctcgt gttgccgacc tcgacctcga
     1141 cgaacacgag atcaccgagc ttcacgctgc cgtcggtgag atcgacttcg tcgccggcga
     1201 ggttgcgata cgtgcgcgtg accgacatgc cattgccgcc gactttgtag tccgcgcctt
     1261 ggcgcacgcc ggacgaattg acgacgagcc agaggccatc ggctgactgc gggatatcga
     1321 tcgtgagcga cttgtactcg ctcgcgcgct gcagcgacca cgtcttgtca ttcgtcttct
     1381 gcttcgtctt gcgcggttgg atcaccgtgc cgtccgcggt gagcgtgcca tcggcgacac
     1441 ccttcgtgcc catgccgacg acccacttgc cgagacccgt cacgccccac acgagctcct
     1501 gcgtgttgta gtagtagctg ggctgaccgc tgaggctctc ggcgacgcgg ttcgcgagcg
     1561 gctcgcccgc gggatcgttg ccgaacaggt cgtggaacat cgagagctgc atcgcgcggc
     1621 gtcgacggtc cgagtagaag ctccagtagt tgtgacggtc cgcgcggatc ggcgacgcgt
     1681 cgacggtctt cagctccttc tcgtagcgac gatcgccagc gaggtagagc gcggccttca
     1741 gcacgtagag gtcctcggcc atctcgttct ggcgatacgt gagcggcagc ttcgcctgct
     1801 gctcggtgat gtgctcgacg agcttcagga tgcgagcctt cttgccctta ccggcgcgcg
     1861 ctagcacgaa gtgcatgtac gactcggctt gctcgttgta gtaccagtac ggcgtctgat
     1921 cgcggaggtt cgactcgtac tcgccgacgc gtttctcgat ccactcgagc acgttgtcga
     1981 gccgatcttg cggcacggcg taaccggcct tcttcgcgtc gagcagcata tgcgtcgcgt
     2041 acgcggtgcc ccaatcttcg ggatgcgtcg agcccggcca gtagccgaag ccaccttccg
     2101 gcgtctccat cgtgaagatg cgattgacgc ccgcgagcac catgtcctcg accttcttcg
     2161 tcgacgtttc gggatcgact tgctcgacga tcgcgccgat gtagaggaga gggcgcgtcg
     2221 acgacgtcgt ctgctcgatg cagccgtacg gatagtggat gagatacttc aagtgatcga
     2281 acgcctcgcc gtacggattc gacgtcagcc agaacgtcgt ctgctcggag agcggcaccc
     2341 agcctttgag cgcggcctgc gacgagaggt cgagcttgcc ggccttcacc tgtatcttct
     2401 gcagcacgcg atctttcggg ccggctggca tgaacgggat gtcgacctcg tcggagacag
     2461 tgaacgttcc ggcgctgccc ttcgcagtcg cggtgacgtg cagcttcgcc gcgcccgccg
     2521 gtagcttcgc cgtcgtctgg aagacgagtg tctcggcgcg accgttatcg atcttgatag
     2581 agccggtgtc cttgccgccg aacgaaagcg gcgcggcggc cgtcttcggc tgcgcgaggc
     2641 ccgcgattgc aatcgccgcg ctgtcgagct tcaccgtcac ctcgagcgga gcgccggaca
     2701 tgttcgtcat gaacaccgga atctccattt gatcgttctg cgtgacgaac cgcgggaacg
     2761 tcacctggag cacgagcgga tccttcacgg tgacttgcgt ctccgcgcga ccgatcttct
     2821 gcgggcctgc cgtgatcacc atgacgcgca gctggccgcg ataggtcggc acgtggaacg
     2881 gcacggtcag cttgccgtcg ctcggcactg cgagcacgcc cgaccacagc gcgaccggct
     2941 tcaccggctg cacgcgtcct gcgtcgagcg gaccgccgcc ggcttcttct tcaccctcgt
     3001 aatcgccacc accggtcttc gacgacgtgc cagcgggctg atgcagcatc gtccagccga
     3061 tcgtctcgta cgtctcgacg ccgagcgcgc gcttcgcgaa cagctgcgcg agtggatcgg
     3121 gagtcttgta gttcgtcagc gagaggatgc cttcgtcgac gacggcaacg acggcaaacg
     3181 tgctcgcggg cgtcggaccg aggtcgagct tcacatcgag tgatgccgac gagcgaatct
     3241 ctttcggcgt ctcgaccttc acggtctgcg tgaactccgt cggcacgacg tgcacgctct
     3301 tcacgccata cgcgcggtcc ggcatgaacg catcgcggct ctcggcgtgc gggtccttga
     3361 tgaggaacgc gctgacgtag acgtttggcg cgaacgcgtc gagcttgaac gtccagctcg
     3421 attcggcgct cgacacgtcc ttccactccg ccgtcacgac gtgatcggtc tcgacggtcc
     3481 agagcacctt gcccttgtac ggtgacttca gcttcgcggt gacggtctcg ccgaccttga
     3541 tgtccgacgg gatgtcgagc ttgagctgcg tcggcttcgc gggacgcggc gtctgatcgt
     3601 agcggctgcc atcgccgtac tcgtagtagt cgtacgagta cgtgccgtcg agcacgagct
     3661 cggtcttggc cttgcccgct ttcacgcgga cgaggtatcc gccgtacgct tcacccggcg
     3721 tgacgtcgaa cgtgaacttg ccgttctcga ctttcacggt ctgcttgcct tcgggcactt
     3781 cgcgcagcca acggtcgtag cgagagtcgc cgctctcgtc gtcgtagccg tacccgtact
     3841 ccgactcgag atgaatcatg tcgatctgta gctgcttcgt cgcattcggc gcgagcttgc
     3901 cgttccagtc gacgaccatg ccctcgacgg tgaacggctc gccggcctcg gcgcggatcg
     3961 ccttcgtgcg cacgccgatg tagaacttct cggggtggac catcatcgag gcggtcttca
     4021 ccgtcgcgcg gccgctgccc gcttcgagga cgctcgcggt cgccgatagc tcgccggtct
     4081 gcgtgaacgt cgtgtcctcg tcgggtgcgg ggcactccat cgtgatgccg cccttcggat
     4141 cgagctgctc gcgtgcttca ccgagctgca cggccttgcc cttcggctcg acgccgtacg
     4201 tcagatccgc gttctcctcg gggacgaacg tcgatggctc gatctgacac gagagctcga
     4261 cgccgctgtc gaccgcgctg ccgccgaaca gataacgcgc gttgatgtcg aacgccatct
     4321 tgccgccgat cagcgcatcc gctgccttcg gctcgatcgc gaccttcatg cgctcgggga
     4381 cgaactcctc gacttgcagg tcgtacgaag agagcggctt gtcggcgacg ctgagcgtca
     4441 cgcgccagtg gccggtgtcc gcgaatgcgg gcaggtcgta gtcgaaagca agcacgccgg
     4501 cggcgttggt cttcagcgtg atgcgcttcg caacacgcgc gcgcgggtcg acgagcttca
     4561 cctcgacggg cagcgcctgg tccggcgcct tgtccttctg atcgcggacg atcgcaacga
     4621 cgtgcgcgtt gtcaccgggg cggtagacgc cgcggtcgct gaagatgctc gcgcgatacg
     4681 gcgtcgatgc gatgtacggc acgcccgacg tgctcgactc cgtcacatct gcgcgcagat
     4741 ctttgtagcg gatgtacgtg aggtcgtcgc cgcggcgcgc gatcagagcg aatggctcgc
     4801 tctcgtcgat atcgtcgtcg gggcgcgtct gcagcgtgc
//
LOCUS       DATLBZ010000130        14402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3046459, whole genome shotgun sequence.
ACCESSION   DATLBZ010000130 DATLBZ010000000
VERSION     DATLBZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3046459"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VMZ53_06980"
     CDS             <1..402
                     /locus_tag="VMZ53_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS28232.1"
                     /translation="IISNDRDCSNCQTLSGFSLWASLYALSPTFLLWNQSMTSFGPNG
                     PSGNLPLNTWVFLAGTYNGQTARLYINGVEANSLDRVAAIGVPHSYPLRIGHQGFNYN
                     GGVDGLVDEVMIFDRPLTTTEIQVIYQALKP"
     gene            complement(492..2111)
                     /locus_tag="VMZ53_06985"
     CDS             complement(492..2111)
                     /locus_tag="VMZ53_06985"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HUS28233.1"
                     /translation="MKRLSTILAAAALAAPAVGCLDEDPLDGEEVKSEDGKADASSLG
                     VFMDASFEGKLLTDSSWNDKQTVQDQLLYTVGQLNGFTAVGRVDKAEVTNITKTTVNG
                     KTQITYKAKLPIIWNKSRGVPTTVDIKLPLDISYAGQEAFATKYKHDCVDFGAHDVDA
                     GSMFYYFRPRNSGCNIAAADVHAVTATLTASPSQTTGKFPEYNKIWEDNTLNVVAIFG
                     KYEDGATTASDAGIDGYNEFVASMKSEFATRNLTTVPASVPANPGVAAPDIEFNATLP
                     DGKKIKIVALLTDNVNTGLSQPAFRARYESLSSRADLIAYNGHAGLGSNIRALASAGK
                     WVQGQYVVVFMNGCDTFAYIDGSLFSAHKAVNPDDTTGSKYIDIVNNGMPSFFANMPE
                     STLAIMRGFLKFDDPQTYEQIFTKISSSQLVMVTGEADNTFTPGGGGTPSAWNGLNET
                     GSLARSVKKSFTTPTLAAGTYEFAMTGTGDVDLYVKVGREPTLSSYDCRPYKTGSNET
                     CKVTLAQPATVGVMVNGYAASSTFKLVGKKL"
     gene            complement(2233..2490)
                     /locus_tag="VMZ53_06990"
     CDS             complement(2233..2490)
                     /locus_tag="VMZ53_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28234.1"
                     /translation="MPRLVRLDTGDEIGKIDDKQLAFLVEQLEEEHDDDKDYFIDRET
                     LELLSDNGADPELLGLLEKGLAGDDEMDISWETDAEAAAKA"
     gene            2535..3563
                     /locus_tag="VMZ53_06995"
     CDS             2535..3563
                     /locus_tag="VMZ53_06995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS28235.1"
                     /translation="MPELVANVDVAIIGGGFAGMATAWALRRLGITNTIVLERENELA
                     RYASGRSAGLGRQLAEDNDTTALTVRGAALMKDMPGVWTPSGGLLTFDDPANAEAYSV
                     RAVRFGIAAEMVDRAFVAERWPVLADMRLSRALWVPGDGTIDVAGLLRTYGEGIHVML
                     GAGLERIEQSETGGPRLVTPKGTVTARVVVDAAGAWAGEITGGEPLTSFKRHIFILDA
                     DVHAAAPWLWHLGTTEMYMRPDGDGVLASPCDVAPSQPGHQAPDLVGEAHLRRVLDGA
                     DSSLATAPITRRWACQRSFTENRKMRLGRDSTRPWLVWAAGLGGHGATASPAVGETVA
                     GEVVKSLS"
     gene            complement(3571..3870)
                     /locus_tag="VMZ53_07000"
     CDS             complement(3571..3870)
                     /locus_tag="VMZ53_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28236.1"
                     /translation="MKRAWLLGLLVVGACGKGPSDDQCKQLLTHIVDIEFKEAGAAAS
                     SDTLKAEIAKQKTSVSEAKSKEFLDTCKKKMAKARVTCALAANDKESMAKCDEQK"
     gene            complement(3875..5548)
                     /locus_tag="VMZ53_07005"
     CDS             complement(3875..5548)
                     /locus_tag="VMZ53_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS28237.1"
                     /translation="MGRAIRCALAFVIAACHHAPPPVSVGPLPPGAYSHYLAGKMALY
                     RDDLDGAVREMSAAVEAAPEQPMISVELARVLAKAKRDDGARDVLARARKKWPYHAQV
                     WLASGEVLEIGSEKTPALRKEALIAYKRAIDLEPAEERGYLGLARTQEAAGDTRGAEK
                     TLRALVAKKPDSVDGHYRLAQRLDEKGERDAAAKELRAVLEWEPDHLDARMDLAHTLR
                     RQGKFADAVTQARSAFDRAGQPMDLAEELYWLICEADDLQGAIDLLTLLDDDRSDADA
                     LATVAQFQRGLGRIAESRVVAEHIKPLDADESVIALAEADISDGKTKEAIDRLQTIGV
                     ASARYLESRRVLVNAYLAAHDPAKALAVITPLRAEKPAQLDLAFAEALTRAEAGETAA
                     AKTLAAGFKGEDMPVAYLRARILDKLGDYKGALAVLEPALVKSPDHVSGLNLAGYLLA
                     QHKERLGDAERYLAHARDLQPGDPSVLDSWGNLLLQRGKSRDAVRALDHAARFAPHEA
                     EILVHLAEAWAADGAPKHAAEILDRAAALKPPSDVIRRIDAIRATLAIR"
     gene            complement(5585..6178)
                     /locus_tag="VMZ53_07010"
     CDS             complement(5585..6178)
                     /locus_tag="VMZ53_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28238.1"
                     /translation="MPRVPDLVICLAHRRWSSYYDRSQELMAQCARERRVVFVEEPEL
                     VDDRDVPAIELAETRCGVVTLIPHMPADLTLQEMERRQRRALDFILAHYGCTNPVLWY
                     YEPKALAYTDHIDAAAVVYDWVDEGPHPVPHLGRRAQQLLDRADVVFTDTADHRQLVH
                     HNVFPTVEAATWAEMWTEMWAVVERAIDARPLQSVAL"
     gene            complement(6235..7788)
                     /locus_tag="VMZ53_07015"
     CDS             complement(6235..7788)
                     /locus_tag="VMZ53_07015"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HUS28239.1"
                     /translation="MKDPIARLEEMEAKAMLAGGQARIDKMHEAGKLTARERISALLD
                     EGSFVELDKFVTHRCADFGMQEQKILGDGVVTGHGTVDGRPVCVFAQDFTVFGGSLSG
                     AYAQKICKVMDLATKIGCPVIGLNDSGGARIQEGVESLAGYADIFLRNVLTSGVVPQI
                     SAIMGPCAGGAVYSPAMTDFILMVERTSYMFITGPEVIKTVTHEEVSKEQLGGAQTHA
                     SKSGVSHFTESDELSCIARIRELLSFMPSNNAEDPPSRATQDPPDRAEASLDSVVPLE
                     SNKPYDIKKIITAVVDDQRFVEVHKDYAKNIVVGFARFDGRPAGIVANQPMHLAGCLD
                     IDASIKAARFVRFCDCFNIPLITFVDVPGFLPGTNQEYGGIIKHGAKLLYAFAEATVP
                     KITVITRKAYGGAYDVMASKHIRADVNVSYPNAEIAVMGSEGAVNIIYRTELDKITDE
                     KARAEARAKFTEQYRETFANPYKAASLGYIDEIIRPRETRWTIIRSLRTLKNKRQANL
                     PRKHGNIPL"
     gene            7812..8369
                     /locus_tag="VMZ53_07020"
     CDS             7812..8369
                     /locus_tag="VMZ53_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28240.1"
                     /translation="MANVKGSALSSRILWVQLEHGEAGFNRLLHQVSPDLKSTLEMGV
                     NKAKWYPFEQFVELNVTLDRLFGKGDLDLIRTLGRFGADANLKTIYRLFYKVGTVHWI
                     LGRAVRLWSAHYDSGFLEVATRGPKAAVLRVRGFATPHPAHCLAVWGWAERSIELSGG
                     KKAVMDQTKCRNRGDDHCQYDATWE"
     gene            complement(8373..12575)
                     /locus_tag="VMZ53_07025"
     CDS             complement(8373..12575)
                     /locus_tag="VMZ53_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HUS28241.1"
                     /translation="MRTTHEEVLDDLAALVAGDSEAIARHADHLSSCDDCRDKKHEAA
                     VTAKLVGDAGADYVPNASLVDALMAKVDADKTAGSAETIQMEAAPVVLPKASQPEITT
                     AKPAAPVAKVASIEAKREQKRQAPSKKLYAAAAIAALAAGTTGIIAIKHRASSSSSTQ
                     TATREGGPLGTVKTISRAAADQGDGLAMKTSSGWKALKPGDVIPAGAELRTDERTRAS
                     IEITGGDMRLVLDHATTVAFDANSARKLKLPSGRIAADVVHDDKHPMSITTPNGVVDD
                     VGTRFSVTATDKVTAVQVVRGEVKLTSKDGASDAVRAGEEGLIENGKLSVNAAPSLVK
                     ENQWSELAPPPAKDSDDSLAGLGALRAYKPGEKRDRDWKLALANHEVKVRIVGPIART
                     EITETFRNDSDTTLEGVYQFPLPADAQIDSLALDMKDEPGGFIAGAFVDKDRATKIWK
                     GVIDKATPKHIQRDPLIMQEIVWVPGPWKDPALLDWKRGGRFELKIYPIPAKGARTIK
                     LSYTQVVTPRGPMRQYVYPLPHSFDGSTVADKMKVDVEVRGAQQGSVRTVGYDFKADA
                     ARNDVTAMTFEQGGFVPRGDLVVDYRSEQGDAELRAWTFQGGAAAGPDDKLAGKKNVG
                     IDPKVVEAQKLVAADARPTAVIALSPKLPRWRESKPRDYVIVIDGSQSMVGERFAKAS
                     ELAAAMVDDMDRRDRFSALVCDSECTRMGDLRTPSAGAAGDLKSWLGAQQPAGSSDVV
                     TALRAGNDELKDADRDRWVLYIGDGFASSGFRRVSDVEKAIAATTGNGSVHISTIGLG
                     SDADSALLAAAARGGGGSYVAWLPGETVHTAALAALESTNGTTLRDAVVELPAGMSEV
                     AAQGGAPGETTTATKLPTLRAGGELLISARIDGNVTGEVVLKGKVAGQKFEQRYPVKL
                     EVSSAPGNGFVPRLWASLAIDQLEREGKGEDRARVVALSQGYGVMSRQTSLLVLESQA
                     MFDAFGVDRSQSAVTWTGEEDIEESTSAGSLQVPADIGGGKAAAAPQPAAMRKADSMA
                     GDDAESARGMMGGAASIRDEKKAPAMDKPAAQTAMPRMPNRGRRITIAMKKVWTRNAA
                     LGSFSGTDASIKKAVATAEDALAKNPDSREKHRALVQALSYAGDLDKAHEIANRWLER
                     DKLDPQALTYIADLLGRDGKRELSLRTLDGLVDLDPDRANLHERMINAFERSGRMQQA
                     CNHRIAMVSLNVKEAAKRAAAAARCLRTLGRGSDAELVMKSLVDDKARTEAEKLATVA
                     PISPRVAGDLVINGKWDGSADLDISLVAPDGSRVSWMGGRTDITVADSTAHDREQLSV
                     KTLRKGNYLIEITRSESTTSPVHGTIDVTVLGQKKAFPFELSGSRTTVGKISVTMTSH
                     LEPVNPDDARWLNQGRGWDGGL"
     gene            complement(12578..13126)
                     /locus_tag="VMZ53_07030"
     CDS             complement(12578..13126)
                     /locus_tag="VMZ53_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS28242.1"
                     /translation="MLALATVSSADPIVEMIHAGRHRDALAACARTHHVILGRLAMAL
                     LGSQADADEAVQETLLRAHRGMASYRAEGSIKAWLCGIARHVCAHMLETRRRGRELLE
                     VVPTEGEARDAFAARRRARVLRDALEKLKPSEREALVLRYVADLSHREIAAACGLDEA
                     AARKRISRALERLRSVMPSEEI"
     gene            complement(13285..>14402)
                     /locus_tag="VMZ53_07035"
     CDS             complement(13285..>14402)
                     /locus_tag="VMZ53_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28243.1"
                     /translation="EEVERQSKQHIAVVPSGRHDFDGAEATVIRIHTADAFSARDEGK
                     SISQARQVIDRFEEEETRLASLSGDQLRMLRKGAQRDSVEMTAEKPRMDRAAAKRPKR
                     DSNIDDDEMTRAREPIDFGRGGRELYDSPFETPAGATRPGEDAYEPALDTPPHQHLTP
                     TDNTRLVAPRDRRTPLPQQAVRAPARAPTVPPPLPRQRPASQAGAPPRNIATPQPMPM
                     AQMPMSPQPLQMAQPSVPQPLQPNLRTPIPQQPMLPTGPAMPHVATPLPQQPGQPLPP
                     HLAPPQFMPSQNAGAYQQGWGPPVAAQMPMRDATPTAPDSYDRPPYGNRPSMPQVAGF
                     RPSNAMRSRTGLKPWVLVVGALIMAALAFVITRAFIG"
BASE COUNT         2259 a         4717 c         4778 g         2648 t
ORIGIN      
        1 atcatctcga acgatcgcga ctgctcgaac tgccagacgc tgagcgggtt ctcgctctgg
       61 gccagcctgt atgcgttgtc gccgacgttc ctgctctgga atcagagcat gacgtcgttt
      121 ggtcccaacg gcccatctgg caacctcccg ctcaacacct gggtcttcct cgccgggacg
      181 tacaacggcc agaccgcgcg cctctacatc aacggcgtcg aggcgaacag cctggatcgc
      241 gttgcagcga tcggcgtgcc gcacagctat ccgctgcgca tcggccacca aggcttcaac
      301 tacaacggtg gagtcgacgg cctcgtcgac gaagtcatga tcttcgaccg cccgctgacg
      361 accaccgaga tccaggtcat ctatcaggcg ctgaagccgt agcgcgcgcc gaaaacggcg
      421 acgcggctcg taggaccatg tcctctggag ccgcgtcaga taagaaaagt cagtctagag
      481 aaagaggcga attagagctt cttgccgacg agcttgaacg tcgagctcgc tgcgtatccg
      541 ttgaccatca cgccgaccgt cgcgggctgc gcgagggtga ccttgcacgt ctcgttcgag
      601 cccgtcttgt aggggcggca gtcgtacgac gacagcgtcg gctcgcggcc aaccttgacg
      661 tagaggtcga cgtcgcccgt gccggtcatg gcgaactcgt aggtgccagc ggcgagcgtc
      721 ggcgtggtga acgacttctt cacgctgcga gcgagcgagc cggtctcgtt gaggccgttc
      781 caggcgctcg gcgtaccgcc gccacccggc gtgaaggtgt tgtcggcttc gccggtcacc
      841 atcacgagct gcgacgacga gatcttcgtg aagatctgct cgtacgtctg cgggtcgtcg
      901 aacttgagga agccgcgcat gatggcgagc gtcgactcgg gcatgttcgc gaagaaggag
      961 ggcatgccgt tgttgacgat gtcgatgtac ttgctgccgg tcgtgtcgtc ggggttgacg
     1021 gccttgtgcg ccgagaagag cgaaccgtcg atgtacgcga acgtgtcgca gccgttcatg
     1081 aagacgacga cgtactggcc ttggacccac ttgcccgccg aagcgagcgc gcggatgttg
     1141 ctgccgagac cggcgtggcc gttgtacgcg atgaggtcag cgcgcgaaga gagggactcg
     1201 tagcgagcgc ggaacgccgg ctgcgagagg cccgtgttga cgttgtcggt gaggagcgcg
     1261 acgattttga tcttcttgcc atcgggcagc gtcgcgttga actcgatgtc cggtgcggca
     1321 acgcccgggt tcgcgggcac cgacgccggg acggtcgtca ggttgcgggt cgcaaattcg
     1381 ctcttcatcg aggcgacgaa ctcgttgtag ccgtcgatgc cagcgtcaga ggcggtggtc
     1441 gcgccgtctt cgtacttgcc gaagatcgcg acgacgttga gcgtgttgtc ttcccagatc
     1501 ttgttgtact cggggaactt tccggtcgtc tgcgacgggc tcgccgtgag cgtcgcggtg
     1561 accgcgtgaa cgtcggccgc tgcgatgttg cagcccgagt tacgcgggcg gaagtagtag
     1621 aacatcgagc cggcatcgac gtcgtgcgcg ccgaagtcga cgcagtcgtg cttgtacttc
     1681 gtcgcgaagg cttcttggcc cgcgtacgag atgtcgagcg ggagcttgat gtcgaccgtg
     1741 gtcggaacgc cgcgcgactt gttccagatg atcggcagct tcgccttgta cgtgatctgc
     1801 gtcttgccgt tgaccgtggt cttcgtgatg ttcgtgacct cggccttgtc gacgcgaccg
     1861 acggccgtga agccgttgag ctggccgacg gtatagagga gctggtcctg aaccgtctgc
     1921 ttgtcgttcc acgacgagtc cgtcaggagc ttgccttcga acgacgcatc catgaacacg
     1981 ccgagcgagc tcgcgtcggc cttgccgtcc tcggacttca cttcttcccc gtccagcggg
     2041 tcttcatcga gacagcccac agcgggggcg gcgagggcgg cggcggcaag gatggtcgac
     2101 aggcgcttca tgcgggcccg tataggcaag gtcggtgcct accgaaggag ccgctaacgg
     2161 cgcgagatca tggcccaccg cgtcgcaact cgactagcca ggaaggggtg cgacaggctg
     2221 cccagctagc cgctacgcct tggcagcagc ttcggcgtcg gtctcccagg agatgtccat
     2281 ctcatcgtcg ccggcgaggc ccttctcgag caggccgagc aactccggat cggctccgtt
     2341 gtcgctgagc agctcgagcg tctcgcgatc gatgaagtag tccttgtcgt cgtcgtgctc
     2401 ttcttcgagt tgctcgacca ggaacgcaag ctgcttgtcg tcgatcttcc cgatctcgtc
     2461 tccagtgtcg aggcgaacca gacgtggcat ggcccgagtc tgccgcaatc ggctcccgtg
     2521 ctacaaggtg cgcggtgccc gagcttgtcg ccaacgtcga cgtcgcgatc atcggcggcg
     2581 gattcgcggg catggcgacg gcctgggcgc tgcgccggct gggcatcacg aacacgatcg
     2641 tgctcgagcg cgaaaacgag ctcgcccgct atgcgagtgg tcggagcgcg ggcctgggcc
     2701 gccagctcgc cgaagacaac gacaccacag cgctgaccgt gcgcggcgca gccctgatga
     2761 aggacatgcc aggggtgtgg acgccgagcg gcggcctgct cacgttcgac gatccagcta
     2821 acgccgaggc gtactcggtg cgcgcggttc gcttcggcat cgccgcggag atggtcgatc
     2881 gtgcgttcgt ggcggagcgc tggccggtgc tcgccgacat gcggctctcg cgcgcactgt
     2941 gggttcccgg cgacggcacg atcgatgtcg cgggtctgct gcgcacgtac ggcgaaggca
     3001 tccacgtgat gctcggtgcc ggtctcgaac ggatcgagca atccgaaacc ggcggtccac
     3061 gcctcgtcac gccaaagggc acggtcaccg cgcgcgttgt cgtcgatgcg gccggcgcat
     3121 gggcgggtga gatcacgggc ggcgagccac tcacgtcgtt caagcggcac atcttcatcc
     3181 tcgacgccga cgtccacgcc gccgcgccgt ggctctggca cctcggcacg acggagatgt
     3241 acatgcgccc cgacggagat ggtgtgctcg cgtcgccgtg cgatgtcgcg ccgagccagc
     3301 ctggtcatca agcgcccgac ctcgtcgggg aagcgcacct gcgtcgcgtg ctcgacggcg
     3361 ccgactcgtc gctcgcgact gcgccgatca cgcggcggtg ggcctgccag cgctcgttca
     3421 ccgagaaccg caagatgcgc ctcggtcgcg actcgactcg gccgtggctc gtgtgggccg
     3481 ccggcctggg cggtcacggc gcaacggcgt cacctgccgt cggtgaaacc gtcgcgggcg
     3541 aagtcgtgaa gtcgctcagc taagacggcg ctacttctgc tcgtcgcact tggccatcga
     3601 ctctttgtcg ttcgccgcga gcgcgcatgt gacgcgcgcc ttcgccatct tcttcttgca
     3661 cgtgtcgaga aactctttgc tcttcgcttc gctgaccgac gtcttctgct tcgcgatctc
     3721 cgctttcagc gtgtcgctcg atgcagcggc accggcttct ttgaactcga tgtcgacgat
     3781 gtgcgtaaga agttgcttgc actgatcgtc ggaggggcct ttgccacacg caccgacgac
     3841 gagaagaccg agcagccaag cgcgcttcat cccattatcg tatcgcaagc gttgcccgaa
     3901 tggcatcgat ccgccggatc acgtcggatg gcggctttag ggcggccgcg cgatcgagga
     3961 tctccgcggc atgcttgggc gcaccgtcgg cagcccaggc ctcggcgagg tgtacgagga
     4021 tctccgcttc gtgcggggcg aagcgtgcag cgtggtcgag cgcacggacg gcgtcgcgtg
     4081 atttaccgcg ctggagcaag aggttgcccc agctgtcgag caccgatgga tcgcccggct
     4141 gcagatcgcg agcgtgggcg aggtagcgtt ccgcatcgcc gagccgctcc ttgtgctgcg
     4201 cgagcagata gccagcgagg ttgagaccac tcacgtgatc gggcgatttc acgagcgccg
     4261 gctcgagcac ggcgagcgcg cctttgtagt cgccgagctt gtcgaggatg cgcgcgcgaa
     4321 gatatgcgac cggcatgtct tcgcccttga agcccgcggc aagcgttttc gccgcggcgg
     4381 tctcgcccgc ttctgcgcgc gtcagcgctt cggcgaatgc gagatccagc tgcgcgggtt
     4441 tctccgcacg aagcggcgtg atcacggcaa gcgccttcgc tggatcgtga gcggcgagat
     4501 acgcattgac gagcacgcga cgcgactcga ggtagcgcgc cgatgcgacg ccgatcgtct
     4561 gcagccgatc gatcgcctcc ttcgtcttgc catcgctgat gtcggcttcg gccagcgcga
     4621 tcacgctctc gtcggcgtcg agcggcttga tgtgctcggc cacaacgcgc gattcagcaa
     4681 tgcggccgag cccgcgctga aactgcgcga ccgtcgccag tgcgtcggca tcgctccgat
     4741 cgtcgtcgag cagcgtcagc agatcgatcg cgccctgcag atcatccgct tcgcagatca
     4801 gccaatacag ctcctccgcg agatccattg gctggcccgc gcgatcgaac gcgctgcgcg
     4861 cttgcgtgac ggcgtccgca aactttcctt gccgccgcag cgtgtgcgcg agatccatcc
     4921 gcgcatcgag gtgatcgggc tcccactcga gcaccgcacg cagctccttg gccgcggcgt
     4981 cgcgctcgcc cttctcgtcg agccgctgcg cgagccggta atggccatcg accgaatccg
     5041 gcttcttcgc gacaagcgca cgcaacgtct tctccgcgcc gcgcgtgtct ccggccgcct
     5101 cctgcgttcg cgcgagaccg agatagccac gctcctccgc cggctccagg tcgatcgcgc
     5161 gcttgtacgc aatcagcgcc tccttgcgca gcgccggcgt cttctccgac ccgatctcca
     5221 gcacctctcc cgacgcgagc cacacctgcg cgtgatacgg ccacttcttg cgcgctctcg
     5281 ccaagacatc gcgggcaccg tcgtcgcgct tcgccttcgc caacacgcgc gcgagctcga
     5341 cggaaatcat cggctgctcg ggcgcagcct ccaccgccgc cgacatctcg cgcaccgcgc
     5401 cgtccagatc atcgcgatac agcgccatct tgccggcgag ataatgcgaa tacgcgcccg
     5461 gcggcagcgg cccgaccgag accggcggtg gcgcgtgatg acacgcagcg atcacgaacg
     5521 ctagcgcgca gcggatcgca cggcccattg cgccgagctt atcagcgaaa tcgcaccgcg
     5581 ctcgttacag cgccacggac tggagaggcc gcgcatcgat cgcgcgctcg acgaccgccc
     5641 acatctccgt ccacatctct gcccacgtcg ctgcttccac cgtcggaaac acgttgtgat
     5701 gcacgagctg gcggtgatcc gcggtatcgg tgaacacgac gtccgcgcga tcgaggagct
     5761 gctgcgcgcg acgccccaag tgcggcaccg gatgcggacc ttcgtcgacc cagtcgtaga
     5821 cgacggccgc cgcatcgatg tgatcggtgt acgcgagcgc cttcggctcg tagtaccaga
     5881 gcaccggatt cgtgcagccg tagtgcgcga ggatgaagtc gagcgcgcga cgctggcggc
     5941 gctccatctc ttgcagcgtc aggtcggccg gcatgtgtgg gatcagcgtc acgacgccgc
     6001 agcgggtctc ggccagctcg atcgcgggga catcgcggtc atcgacaagc tcgggctcct
     6061 cgacaaaaac gacacggcgc tcacgcgcac actgcgccat gagctcctgc gaacgatcgt
     6121 agtaactgct ccagcggcga tgcgcgaggc agataacgag gtcagggacg cgtggcatgg
     6181 cgcatcagac tgcatctgac atgccgccgc ccccgtgcgc aggcgctaca gcgcctagag
     6241 cgggatattg ccgtgcttgc gcgggaggtt cgcctggcgc ttgttcttca gcgtgcgcag
     6301 cgagcgaatg atcgtccacc gcgtctcgcg cggccggatg atctcgtcga tgtagccgag
     6361 cgacgccgcc ttgtaggggt tcgcgaatgt ctcgcggtat tgctcggtga acttggcgcg
     6421 agcttcggcg cgggctttct cgtcggtgat cttgtcgagc tcggtgcggt agatgatgtt
     6481 gaccgcgcct tcgctgccca tcaccgcgat ctcggcgttc ggatagctga cgttgacgtc
     6541 ggcgcggatg tgcttcgacg ccatgacgtc gtacgcgccg ccgtacgcct tgcgcgtgat
     6601 caccgtgatc ttcgggacag tcgcttcggc gaacgcgtag agcagcttcg ctccgtgctt
     6661 gatgatgccg ccgtattctt ggttcgtgcc gggaaggaag cctggaacgt cgacgaacgt
     6721 gatgagcggg atgttgaagc aatcgcagaa gcggacgaag cgcgcggctt tgatcgacgc
     6781 gtcgatgtcg aggcagccag ccagatgcat cggctggttc gcgacgatgc cggcggggcg
     6841 accgtcgaag cgcgcgaaac cgacgacgat atttttcgcg tagtctttgt ggacctcgac
     6901 gaagcgctgg tcgtcgacga ccgcggtgat gatcttcttg atgtcgtacg gcttgttcga
     6961 ctcgagcgga acgacggaat cgagcgacgc ctcggcgcga tcgggcggat cttgcgttgc
     7021 gcgcgacggc ggatcctcgg cgttgttgct cggcatgaac gagagcagct cgcggatgcg
     7081 cgcgatgcac gacagctcgt cgctttcggt gaagtgagag acgcccgact tcgacgcgtg
     7141 cgtctgtgcg ccgccgagct gctccttcga gacctcctcg tgagtgaccg tcttgatcac
     7201 ctcggggccc gtgatgaaca tgtagctcgt gcgctcgacc atcaggatga agtcggtcat
     7261 cgcgggcgag tagacggcgc cgcctgcgca cgggcccatg atggcggaga tctgcggcac
     7321 gacaccgctc gtcaggacgt tgcgcaggaa gatgtcggca tagcccgcga gcgactcgac
     7381 gccttcttgg atacgcgcgc cacccgaatc gttgaggccg atgacggggc agccgatctt
     7441 cgtcgcgagg tccatcacct tgcagatctt ctgggcgtac gcgccggaca gcgagccgcc
     7501 gaagaccgtg aagtcttgtg cgaacacgca gaccgggcgg ccgtcgaccg tgccgtgccc
     7561 cgtgacgacg ccgtcgccta gaattttctg ctcctgcatg ccgaagtcgg cgcagcgatg
     7621 cgtgacgaac ttatcgagct cgacgaagct gccttcatcg aggagcgcgc tgatgcgctc
     7681 gcgcgccgtc agcttgccgg cctcgtgcat cttgtcgatg cgggcctgcc ctccggcaag
     7741 catggccttg gcctccatct cctcgagtcg tgcgatcgga tctttcatag gaaggcgcgt
     7801 atagtagtgg cgtggcgaac gtcaagggaa gcgcgctctc gtcgcgcatc ctgtgggtgc
     7861 agttagagca tggtgaagcg gggttcaatc gcctgctcca ccaggtgtct cctgacctca
     7921 agtcgacgct cgagatgggc gtcaacaagg ccaagtggta cccgttcgag cagttcgtcg
     7981 agctcaacgt caccctcgac cggctcttcg gcaaaggcga tttggacctg attcgcacgc
     8041 tcggccgatt cggggcggac gcgaatctaa agacgatcta ccgcctgttc tacaaggtcg
     8101 gcaccgtgca ctggatcctc gggcgagcgg tgcgcctgtg gagcgcccac tacgactcgg
     8161 gcttcctcga ggtggcgacg cgcgggccga aggcagccgt cctgcgcgtc cgcggctttg
     8221 cgacgccgca tcccgcacac tgtctcgcgg tgtggggatg ggcggagcga tccatcgagc
     8281 tatcgggcgg caagaaggcc gtcatggatc agacgaagtg ccgcaaccgc ggcgacgatc
     8341 actgtcagta cgacgcgacc tgggagtagc ggctagagac cgccgtccca accgcggccc
     8401 tggttgagcc agcgcgcgtc gtccgggttc accggctcga ggtgcgacgt catcgtcacc
     8461 gagatctttc cgacggtcgt gcgcgagcct gacagctcga acgggaacgc cttcttctga
     8521 ccaaggacgg tcacgtcgat ggtgccgtgg accggcgagg tcgtcgattc cgagcgcgtg
     8581 atctcgatga ggtagttgcc cttgcgcagt gtcttcaccg agagctgctc gcgatcgtgt
     8641 gccgtcgagt ccgccacggt gatgtcggtg cggccaccca tccacgacac gcgcgagcca
     8701 tccggcgcga cgagcgagat gtcgaggtcc gcactgccgt cccacttgcc gttgatgacg
     8761 aggtcaccgg cgacgcgcgg gctgatcggc gcgaccgtcg cgagcttctc ggcttcggtg
     8821 cgcgccttgt cgtcgacgag cgacttcatg acgagctccg catccgagcc acgaccgagc
     8881 gtacgcaggc agcgagctgc tgcagctgcg cgcttcgccg cttctttgac gttgagcgag
     8941 accatcgcga tgcggtggtt gcacgcctgc tgcatgcggc ccgaacgctc gaacgcgttg
     9001 atcatgcgct cgtggaggtt cgcgcggtcc ggatcgagat cgacgagacc gtcgagcgtg
     9061 cggagcgaga gctcgcgctt gccgtcgcga ccgaggaggt cggcaatgta cgtgagcgct
     9121 tgcgggtcga gcttgtcgcg ctcgagccag cggttcgcga tctcgtgcgc cttgtcgagg
     9181 tcacctgcgt acgacagcgc ctgcacgagc gcacggtgct tctcgcggct gtcgggattc
     9241 ttcgcgagcg cgtcttctgc cgtcgcgacc gccttcttga tcgatgcgtc ggtgcccgag
     9301 aacgagccca gtgccgcgtt gcgcgtccag actttcttca tcgcgatggt gatgcgacgg
     9361 ccgcggttcg gcatgcgagg cattgcggtc tgtgccgccg gcttgtccat cgccggcgcc
     9421 ttcttctcgt cgcgaatcga ggcggctcca cccatcatgc cgcgcgcgga ctccgcgtcg
     9481 tcaccggcca tcgagtctgc tttcctcatc gcggccggct gcggtgccgc cgccgctttg
     9541 ccaccgccga tgtccgcggg aacttgtagc gagccggcgg aggtcgactc ttcgatgtct
     9601 tcctcgccgg tccacgtgac ggccgactgg ctgcgatcga cgccgaatgc atcgaacatc
     9661 gcctgcgact cgagcacgag cagcgacgtc tggcgcgaca tcacgccgta gccttgcgac
     9721 agcgcgacca cgcgtgcgcg gtcctcgcct ttgccttcac gctcgagctg atcgatcgcg
     9781 agcgacgccc acaggcgcgg cacgaagccg ttgcccggcg ccgagctgac ctcgagcttc
     9841 accgggtaac gctgctcgaa cttctggccg gcgaccttgc ccttgagcac gacctcgccc
     9901 gtgacgttgc cgtcgatgcg agcagagatg agcagctcac cacccgcgcg cagcgtcggc
     9961 agcttcgtcg ccgtcgtcgt ctcgcccggg gcgccacctt gcgcagccac ttcgctcatg
    10021 ccggccggca gctcgaccac tgcatcacgc agcgtcgtgc cattcgtcga ctcgagcgca
    10081 gcaagcgccg ccgtgtgcac cgtctcgccc ggaagccacg ccacgtacga gccgccacca
    10141 ccgcgagctg ctgcagcgag cagtgcggaa tcagcatcgc tgccgagacc gatcgtcgag
    10201 atgtgaactg atccgttgcc cgtcgtcgca gcaatcgcct tctcgacgtc cgagacgcga
    10261 cggaagccgc tcgacgcgaa accatcacca atgtagagga cccagcggtc gcggtcggcg
    10321 tccttgagct cgtcattgcc agcgcggagc gcggtcacga cgtcgctcga gcccgcaggc
    10381 tgctgcgcac cgagccacga cttgaggtca cctgcggcac cggccgacgg cgtacgcaga
    10441 tcacccatgc gcgtgcactc gctatcgcag acgagcgcgc tgaagcgatc gcggcgatcc
    10501 atgtcatcga ccattgcggc tgcgagctcg ctcgccttcg cgaagcgctc gccgaccatc
    10561 gattggctgc catcgatcac gatgacgtag tcgcgcggct tggactcgcg ccaacgcggg
    10621 agcttcggcg agagcgcgat gaccgccgtc gggcgtgcgt ccgcagcgac gagcttctgc
    10681 gcctcgacga ccttcggatc gatgccgaca ttcttcttgc ccgcgagctt gtcgtcgggg
    10741 cctgcggcag cgccgccctg gaacgtccag gcgcggagct cggcatcccc ttgctccgag
    10801 cgataatcga cgacgagatc gccccgcggc acgaagccac cctgctcgaa cgtcatcgca
    10861 gtgacatcgt tgcgcgctgc gtctgccttg aagtcgtagc cgacggtgcg gacgctgcct
    10921 tgctgggcac cgcgcacttc gacgtcgacc ttcatcttgt ccgcgaccgt cgagccgtca
    10981 aacgaatgcg gcagcgggta cacgtactga cgcatcggcc cgcgcggcgt gacaacctgc
    11041 gtgtacgaca gcttgatcgt gcgcgcgccc ttcgcgggaa tcggataaat cttcagctcg
    11101 aaccggccgc cgcgcttcca atcgaggagc gctggatcct tccagggacc ggggacccac
    11161 acgatctcct gcatgatcag cgggtcgcgc tggatgtgct tcggcgtcgc cttgtcgatg
    11221 acgcccttcc agatcttcgt cgcgcgatct ttgtcgacga acgcgccggc gatgaagcca
    11281 ccgggctcgt ctttcatgtc gagcgcgagg ctatcgatct gcgcatccgc cggcagcggg
    11341 aactgataga cgccttcgag cgtcgtgtcg ctgtcgttgc ggaacgtctc ggtgatctcg
    11401 gtgcgagcga tgggaccgac gatgcggacc ttcacttcgt gattggcgag cgcgagcttc
    11461 caatcgcgat cgcgcttctc gccgggcttg tacgcgcgca aggcgccgag gcccgcgagc
    11521 gagtcgtcgg agtctttcgc cggcggcggc gcgagctccg accattgatt ctctttgacg
    11581 agcgacggcg ccgcgttcac cgacagcttg ccgttctcga tgaggccttc ttcgcccgcg
    11641 cgaaccgcat cgctcgcgcc gtccttcgac gtgagcttca cttcgccgcg aacgacctgc
    11701 acggcagtga ccttgtcggt cgccgtgacg gaaaaccgcg tgccgacgtc gtcgacgaca
    11761 ccgttcggcg tcgtgatcga catcggatgc ttgtcgtcgt ggacgacgtc agccgcaatg
    11821 cggcccgacg gcagcttcag cttgcgcgcg ctgttcgcat cgaacgcaac ggttgtcgcg
    11881 tggtcgagga cgaggcgcat gtcgccgccc gtgatctcga tcgacgcgcg cgtacgttcg
    11941 tccgtgcgca gctcggcacc ggcgggaatg acatcgccgg gctttagcgc cttccaaccc
    12001 gacgaggtct tcatcgcgag gccatcgcct tgatcggcag cggcgcgaga gatcgtcttg
    12061 acggtgccga gcggaccacc ctcgcgggtc gcggtctgcg tcgacgacga ggagctcgcg
    12121 cgatgcttga tcgcgatgat cccggtcgtg ccggcggcaa gcgctgcgat tgcagcggcc
    12181 gcgtagagct tcttgctcgg ggcttggcgc ttctgctcgc gcttcgcttc gatgctggcg
    12241 acctttgcga caggagcggc cggcttggcg gtcgtaatct ccggttgcga tgccttcgga
    12301 agcaccaccg gtgcagcctc catctggatc gtctcggcgg agccggccgt cttgtcggca
    12361 tcgaccttcg ccatcagcgc atcgacgagg ctcgcgttcg gcacgtagtc tgcgccggca
    12421 tcaccgacga gcttcgccgt aaccgcggcc tcgtgcttct tgtcgcgaca atcgtcgcag
    12481 ctggagaggt gatcggcatg acgcgcgatc gcttcggaat cgccagcgac gagcgcggcg
    12541 agatcatcga gaacttcttc gtgggtggta cgcatcttta gatctcctcg gagggcatca
    12601 cggagcgaag ccgctcgagc gcgcgactga ttcgcttacg cgcagcggct tcgtcgagcc
    12661 cgcaggccgc cgcgatttcg cgatggctca ggtcagcgac gtagcgaagg acgagcgctt
    12721 ctcgctcgct gggcttgagc ttctcgagtg catcgcgcag gacgcgcgcg cgccggcggg
    12781 cagcgaacgc atcacgcgct tcgccttccg taggcaccac ttcgaggagc tctcgaccgc
    12841 gacgacgcgt ctcgagcatg tgagcgcaca cgtgacgcgc gatgccacac aaccacgcct
    12901 tgatcgatcc ctcggcgcgg tacgacgcca ttccgcggtg tgcgcggagc agcgtctctt
    12961 gtaccgcctc gtcggcatcg gcctgcgatc ccagaagcgc cattgcaagg cgaccgagga
    13021 tcacgtggtg ggtgcgggca cacgcggcaa gtgcatcgcg gtggcgaccc gcgtggatca
    13081 tctccacgat cggatcggcg gacgacacgg ttgccaacgc cagcatctca gtccccatgt
    13141 gcagaccggc gttggtttcg tgaccgcctt tttccgataa ctcggaaccc cgcgaaactc
    13201 ttggctcgtc gttgagaccc attgggtttc aaacggccgg tgtcgaggcc cgatctaacg
    13261 agcgggtgtg cggaatgtag gtagctaccc gataaacgcg cgcgtgatca cgaacgcgag
    13321 cgcggccatg atcagtgcac cgacgacgag aacccacggc ttgaggccgg tgcgcgatct
    13381 catcgcattg ctcgggcgaa agcccgcaac ctgcggcatc gacggtcgat tgccatacgg
    13441 aggacggtcg taagaatccg gcgcggtcgg agtcgcatcg cgcatcggca tctgcgcggc
    13501 gaccggaggt ccccatcctt gttgatacgc gcccgcgttc tgcgacggca tgaactgcgg
    13561 cggcgcgaga tgcggtggca gcggttgccc gggctgctgc ggcagcggtg tcgcgacgtg
    13621 cggcatcgcg gggcccgtcg gcagcatcgg ttgttgcgga atcggggtgc gtaggtttgg
    13681 ctgcaacggc tgcggtaccg acggctgcgc catctgcaat ggctgcggcg acatcggcat
    13741 ctgggccatc ggcatcggct gtggcgtcgc gatgtttcgc ggcggcgcgc ccgcttgcga
    13801 tgcaggacgc tgacgcggca gaggtggcgg caccgtcggt gcacgtgcag gcgcgcgcac
    13861 ggcttgttgc ggcagcggcg tgcggcgatc gcgtggagcg acgagacgcg tgttgtccgt
    13921 cggcgtgagg tgctggtgcg gcggtgtgtc gagcgcaggc tcgtaggcat cttcgccggg
    13981 cctcgtcgcg ccggcgggtg tctcgaacgg cgagtcgtag agctcacggc cgccgcgccc
    14041 gaaatcgata ggctcgcgcg cgcgagtcat ctcgtcgtcg tcgatattgg agtcgcgctt
    14101 cgggcgcttc gcagcggcgc ggtccatgcg cggcttctcc gcggtcatct cgacggagtc
    14161 gcgctgcgcg cctttgcgca gcatgcggag ctgatcgccg gagagcgatg cgagccgcgt
    14221 ttcctcttct tcgaagcgat cgatcacctg acgcgcttgc gagatgctct tgccttcgtc
    14281 gcgcgcgctg aaagcatccg ccgtgtgaat gcggatgacg gtggcttccg cgccgtcgaa
    14341 gtcgtggcgc cccgacggca cgaccgcgat gtgctgcttg ctctggcgct cgacttcctc
    14401 tg
//
LOCUS       DATLBZ010000131         6545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2364935, whole genome shotgun sequence.
ACCESSION   DATLBZ010000131 DATLBZ010000000
VERSION     DATLBZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364935"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VMZ53_07040"
     CDS             <1..481
                     /locus_tag="VMZ53_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS28244.1"
                     /translation="NPDNAFAWNNLGYTELLLKRYEDAAEHLGEATTKKDATGYMFNN
                     LGTALEHMDNLDEARLAYEAGGKLGSKEASLSRTRLDGVKTIVVVKDEEVVGVVHDEV
                     KGEAPKGVNEYELTNEGDIEPTEEPVAEQVKSDDGEESVDDSEQAPVVEEPSDTATH"
     gene            complement(475..2076)
                     /locus_tag="VMZ53_07045"
     CDS             complement(475..2076)
                     /locus_tag="VMZ53_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HUS28245.1"
                     /translation="MSAKNDDRKPKLRKAHFATDAEIEIKGAYGPDDGSGDATPPGQF
                     PFTRGVQPTMYRGQLWTMRQYAGFGTASESNKRYKYLLAQGGKGLSVAFDLPTQMGRD
                     SDHMMARGEVGRVGVAIDSLDDMRVLFGGIPLADVSTSMTINATAATLLALYIAVGEE
                     QGVAAKDLRGTIQNDILKEYIARGTYIYPPAGSMRLITDTFTYCSKDVPQWNTVSISG
                     YHIREAGSDAVQEVAFTLADGMAYVKAAIDAGLAVDDFASRLSFFFNGHNNFLEEVAK
                     FRAARRMWARIMRDRFGAKSPKSQTLRFHTQTAGVTLQAQQPLVNVVRVTVQALAAVL
                     GGTQSLHTNSYDEALGLPTEEAARIALRTQQVIGHESGVADFIDPLGGSWAVEALTDE
                     IEKRAQAYLDEIEKLGGMVAAIEKGFPQKEIERRAYEHQRAVEGKQRIVVGVNDFLVE
                     EEEPVPVAKVDPALEREQCERVAATRARRNAAEHQRALTALDDAAQGTANLLPPIIGA
                     VKAMATVGEIADVLRKRFGEFHPAQ"
     gene            2116..2442
                     /locus_tag="VMZ53_07050"
     CDS             2116..2442
                     /locus_tag="VMZ53_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28246.1"
                     /translation="MRSTAVIVFIGIVGAIAGFCAQKRTVARGDALATELMESNKLIQ
                     SMKCDDHVPIGVDGANFGCKVSFKNGDVADYKFKIDREGSIIATDHGETTRKPHEKKT
                     DDAWGD"
     gene            2442..3518
                     /locus_tag="VMZ53_07055"
     CDS             2442..3518
                     /locus_tag="VMZ53_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28247.1"
                     /translation="MKGLLLCAALVGCAPNATYGYQYQLTQQFTGGEEPEAPGVEARQ
                     LLAGAKTVAFYPPDTCLNAETVVANRKLQEVRANCGVLMSTLERAAQRAGYEVLSWQN
                     LRGGRAIDFAREGKVDVLFEINEFEITPLDDNAVERSMRFFEVHEDGNETALPVTQAT
                     GAQCANYAKAHDKTRTVAVTGSIDIKTVNVSDGRDRWHYRKTREKQFQAEYPRSTFTG
                     KGKTHPLERGLRGVFVGALILAVDLIILDELLNDDPTTMDLDENFDPSPWQWVSAGVA
                     GASLGGMIAVHYKLPVKPEASKVLCNVTTADAAKTVVQVGTLSAQHTFTEVQTGDITK
                     RDYEQVRDEMIGDFIKILSEAHPH"
     gene            3550..4734
                     /locus_tag="VMZ53_07060"
     CDS             3550..4734
                     /locus_tag="VMZ53_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28248.1"
                     /translation="MRRALAVVAALSTLAGCAGTATYSYQYKMQQEFTGGEEPEPAPV
                     EARQLLANAKTVAFYPPDQCINSDPSITGRKLQELKASCGVMMSTLERAAQDAGYEVL
                     SWQNLRGSKRAIEYAREANVDVLFEINDFEHDVLLDNQVQHTFEFFERGRDGNDIPLQ
                     VATPVAQQCANYAFQADKPAPAAQTGAIDIKTVSVVDGRDRWHYRKTEQVAIERDRPR
                     VMFSAPMQPNKGAVTLITIGGVGLALGGTLFAIEKVTKDNPLTPDDEAFSTGGWAARG
                     MIISGLLIAGGIALQVGLGGSKPAPSSVLCDGTWAAPATHAAPVQDNSTSSQYTFHSG
                     TVAPPQSGANLSETQRRMLKQFIDTIVEARKSAHPAPAPAPAPAPAAPAPAPAAPTPP
                     TP"
     gene            4750..5013
                     /locus_tag="VMZ53_07065"
     CDS             4750..5013
                     /locus_tag="VMZ53_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28249.1"
                     /translation="MPNYSKLHDLISHRVLMEYDTGAKVTGYLASCQPSSGPVEFVVL
                     QDVKLIDSRGRVVRETGELTFCPNVLTTIALDEGPRGRDLKVG"
     gene            complement(5010..5477)
                     /locus_tag="VMZ53_07070"
     CDS             complement(5010..5477)
                     /locus_tag="VMZ53_07070"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HUS28250.1"
                     /translation="MTQALFANQRETNLQSMIALIEEVLGELGHEPSAARASETQALH
                     AWRIRKGSALTRVTLLDRAEFTHLRVCSIVMTLDKEVDRPALHQKLLELNSTLCGAAF
                     AIDEEDHVLLLAERSCLDLDRSEVRELIRRVTTYADDHDDTLVAWFGGKMGGA"
     gene            5509..>6545
                     /locus_tag="VMZ53_07075"
     CDS             5509..>6545
                     /locus_tag="VMZ53_07075"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS28251.1"
                     /translation="MSEEPAEERPPSRKRLVILGAAALVVVTLAVIAIVLFGKKHPAS
                     HPLALRKDGGAATHAPIGPKLPGTGVALTGVVVDGAGLPVAGATIEAELEKGMQDRAL
                     ATADAGVPSQDAGVASPSAPTGNDGRFLVDGLDAGRYRLRVTGTGLLAAEVRMVPVPS
                     DELRIVVARQVDIAGVVTDEGKPIVNATVGIRGDAIGGTLETKTDAAGKFNVPNLPEG
                     RYQVYAYRGPLAARTMRVARLGAGPFAPVELRLEAGQNVSGRVIDRGEGTGLVAAIEL
                     RPVGEDQAPRYARSTDDGSFIIEGVPNGQWIADAFSPGYISPGGVELTAGQGIVELAM
                     ARGATIEGRVL"
BASE COUNT         1178 a         2088 c         2192 g         1087 t
ORIGIN      
        1 gaacccggac aacgcgtttg cgtggaacaa cctcggctac accgagctgc tcctcaagcg
       61 ctacgaagac gccgccgagc atctcggcga agcgaccacg aagaaggacg cgaccggcta
      121 catgttcaac aacctcggca ccgcgctcga gcatatggac aacctcgacg aggcgcgtct
      181 ggcctacgag gcgggtggca agctcggctc gaaggaagcg tcactctctc gcacgcgtct
      241 cgatggtgtg aagacgatcg tcgtcgtgaa ggacgaggag gtcgtcggtg tcgtgcacga
      301 cgaggtcaag ggcgaagcgc cgaagggcgt caacgagtac gagctcacga acgaaggcga
      361 catcgagccg acggaagagc ccgttgcgga gcaggtgaag tcagacgacg gtgaagagtc
      421 ggtcgacgac agcgagcagg ctcctgttgt cgaagagccg agcgacaccg cgactcactg
      481 agccgggtga aactcgccga agcgtttgcg taggacgtcg gcaatctcgc cgaccgtcgc
      541 catcgccttc accgcaccga taatgggcgg caggaggttc gcagttccct gagcagcatc
      601 atcgagcgcc gtaagggctc gttggtgctc tgcggcgttt cggcgtgcgc gcgtagcagc
      661 aacccgctcg cactgttcgc gctcgagcgc cggatcgacc ttggcgacgg gcaccggctc
      721 ctcctcctcg acgaggaaat cgttcacgcc gacgacgatg cgttgcttgc cctcgacggc
      781 gcgttggtgc tcgtacgcgc gacgctcgat ctctttttgc gggaagccct tttcgatcgc
      841 cgccaccatg ccgccgagct tctcgatctc gtcgaggtac gcctgcgcgc gcttctcgat
      901 ctcgtcggtg agcgcttcga cagcccacga gccgccgagt ggatcgatga agtcggcgac
      961 accgctctcg tgcccgatca cttgctgcgt gcgcagcgcg atgcgagcgg cctcctcggt
     1021 cggtaggccc agcgcttcgt catagctgtt cgtgtgcagc gattgcgtac cgccgagcac
     1081 cgcagcgagc gcctgcacgg tgacgcgcac gacgttgacg agaggctgct gcgcttgcag
     1141 cgtgacgccc gcggtctgcg tgtggaagcg cagcgtctgg ctcttggggc tcttcgcgcc
     1201 gaatcggtcg cgcatgatcc tggcccacat ccgacgcgcg gcgcggaact tcgcaacctc
     1261 ctcgaggaag ttgttatggc cgttgaagaa gaacgacagg cgcgatgcaa agtcgtcgac
     1321 ggccaggccg gcgtcgatcg cagccttcac gtatgccatg ccgtcggcga gcgtgaacgc
     1381 gacctcttgg accgcatcgg acccggcctc gcgaatgtgg taaccgctga tcgagacggt
     1441 gttccactgc gggacgtcct tgctgcagta cgtgaacgtg tccgtgatga gtcgcatcga
     1501 gcctgcgggc ggatagatgt acgtgccgcg cgcgatgtac tccttgagga tgtcgttctg
     1561 gatcgtgccg cgcagatcct tcgcagcgac tccttgctct tcgccgacgg cgatgtagag
     1621 cgccaggagc gtcgcggcgg ttgcgttgat cgtcatcgag gtcgagacgt cggcgagcgg
     1681 gatgccgccg aacagtacgc gcatgtcgtc gagcgagtcg atcgccacgc cgacgcgtcc
     1741 gacctcgccg cgcgccatca tgtggtccga atcgcggccc atctgcgtcg gcagatcgaa
     1801 ggcgaccgag aggcctttgc cgccctgtgc gaggaggtac ttgtagcgct tgttcgattc
     1861 gctcgcggtg ccgaagcccg cgtattggcg catcgtccac agctggccgc ggtacatcgt
     1921 cggctgcacg ccgcgggtga atgggaactg accgggcggc gtggcatcac cacttccgtc
     1981 gtcgggaccg taggcgcctt tgatctcgat ctcggcgtca gtggcgaagt gggccttgcg
     2041 gagctttggc ttgcgatcgt cgttcttcgc gctcacgggc cgtgtgtagc agagatcgga
     2101 tacaatgagc ctctaatgcg ctccacggcg gtcatcgtgt tcatcggcat cgttggggca
     2161 atcgccggct tctgtgcgca gaagcgcacc gtggcacgcg gcgacgctct ggcgaccgag
     2221 ctcatggagt cgaacaagct gatccagagc atgaagtgcg atgaccatgt gccgatcggc
     2281 gtcgacggcg ccaacttcgg ctgcaaggtc tcgttcaaga acggcgatgt cgccgactac
     2341 aagttcaaga tcgaccgcga gggctcgatc atcgcgaccg accacggtga gaccacgcgc
     2401 aagccgcacg agaagaagac agacgacgcc tggggggact agtgaaggga ctactgctgt
     2461 gtgcagcgct tgttggctgc gcaccgaacg cgacgtacgg ataccagtat cagctcacgc
     2521 agcagttcac gggcggggag gaaccggagg cgcccggggt ggaagcgcgc caactcttgg
     2581 caggcgcgaa gacggtcgcg ttctacccgc cggatacgtg tctcaatgcg gagactgtcg
     2641 tcgcaaatcg caagctccaa gaggtgcgtg cgaactgcgg cgtgctgatg tcgacgctcg
     2701 agcgcgccgc gcagcgtgcc ggctacgagg tgctgtcgtg gcagaacctt cgcggcggcc
     2761 gtgcgatcga ttttgcgcgc gagggcaagg tcgacgtcct gttcgagatc aacgagttcg
     2821 agatcacgcc gctcgacgac aacgcggtcg aacgctcgat gcggttcttc gaggtccacg
     2881 aggacggcaa cgagacggcg cttcccgtga cgcaagcgac cggcgcgcag tgcgcgaatt
     2941 acgcgaaggc gcatgacaag acgcgcaccg tggcggtcac cggctcgatc gacatcaaga
     3001 cggtcaacgt gtccgatggg cgcgaccgct ggcattaccg caagacgcgc gagaagcagt
     3061 tccaagccga gtatccccgc tcgacgttca ccggcaaggg caagactcat ccgctcgagc
     3121 gaggcctgcg cggcgtgttc gtcggtgcgc tgatcttggc ggtcgatctc atcatcctcg
     3181 acgagctctt gaacgacgac ccgacgacga tggaccttga cgagaacttc gatccttccc
     3241 cctggcagtg ggtcagcgcg ggcgtggcgg gcgcgtcgct cggcggtatg atcgcggtgc
     3301 actacaagct cccggtcaag cccgaagcat cgaaggtcct gtgcaacgtg acgacggccg
     3361 acgcggccaa gaccgtggtc caggtcggca cgctgtcggc gcagcacacg ttcaccgagg
     3421 tccagaccgg cgacatcacg aagcgcgact acgagcaggt ccgcgacgag atgatcggcg
     3481 acttcatcaa gatcctctcc gaagcgcacc cgcattgacg cggagccgtc cgtgaccgat
     3541 cgtggtcaca tgagaagagc gttggcggtg gtggcggcgc tgagcacgct cgcgggttgc
     3601 gcggggactg cgacgtacag ctaccagtac aagatgcagc aggaattcac gggtggcgag
     3661 gagcccgagc cggcgcccgt cgaagcgcgg caactgctcg cgaatgcgaa gacggtcgcg
     3721 ttctatccgc cggaccaatg catcaactcc gatccttcga tcacgggtcg caagctgcaa
     3781 gagctcaagg cgagctgcgg cgtgatgatg tcgacgctcg agcgtgcggc gcaggatgcc
     3841 ggctatgaag tgctgtcgtg gcagaacctg cgcggtagca agcgagcgat cgaatacgcg
     3901 cgcgaagcga acgtcgacgt gctgttcgag atcaacgatt tcgagcacga cgttctgctc
     3961 gataaccagg tccagcacac gttcgagttc ttcgagcgag gacgcgatgg caacgacatc
     4021 ccgctgcagg tcgcgacccc ggtcgcccag cagtgcgcga actacgcgtt ccaggcggac
     4081 aagcccgcac ccgctgcgca gaccggcgcg atcgatatca agaccgtctc ggtcgtcgac
     4141 ggtcgcgacc gctggcacta ccgcaagacc gagcaggtcg cgatcgagcg cgacaggccg
     4201 cgggtcatgt tctctgcgcc gatgcagccg aacaaaggtg cggtcacgct catcacgatc
     4261 ggtggcgtcg gcctggccct cggcggcacg ttgttcgcga tcgagaaggt gaccaaggac
     4321 aatccgctca cgcccgacga cgaagcgttc tcgacgggcg gctgggcagc gcgcggcatg
     4381 atcatcagcg ggctcttgat cgcgggcggt atcgcgctgc aggtcggcct cggtggcagc
     4441 aagccggcgc cgtcgtcggt gctgtgcgac ggcacctggg ccgctccggc cacgcacgcg
     4501 gcccccgtgc aggacaactc gacgtcgagc caatacacgt ttcactcggg gacggtcgcg
     4561 ccgccgcaat cgggcgcaaa cctctcggag acgcagcgcc gcatgctgaa gcagttcatc
     4621 gacacgatcg tcgaagcccg aaagtccgct cacccggctc cggctccggc tccagctcca
     4681 gctccggctg cacccgcacc tgcgccagcg gccccaacgc cgccgacgcc gtgatatagg
     4741 aatcctgcga tgccgaatta ctcgaagctt cacgacctga tcagccatcg cgttctgatg
     4801 gagtacgaca ccggcgcgaa ggtcaccggc tatctcgctt cgtgtcagcc gagctccggg
     4861 cccgtcgagt tcgtcgtgct gcaagacgtc aagctcatcg attcgcgcgg ccgcgtggtt
     4921 cgcgagaccg gcgagctgac gttctgcccg aacgtgctga ccacgatcgc gctcgacgaa
     4981 ggtccgcgcg gtcgcgatct caaagtcggc taggcgccgc ccattttgcc gccgaaccag
     5041 gcgacgagtg tgtcgtcgtg gtcgtcggca tacgtcgtca cgcgccggat cagctcgcgc
     5101 acctcgctgc gatcgaggtc gaggcacgag cgctcggcga gtaggaggac atggtcttcc
     5161 tcgtcgatcg cgaacgcagc gccgcacagc gtcgagttga gctcgagcag cttctggtga
     5221 agcgccggac ggtcgacctc cttgtcgagc gtcatgacga tcgagcacac gcgcagatgc
     5281 gtgaactcgg cacgatcgag cagcgtgaca cgcgtgagcg cggatccctt gcggatgcgc
     5341 caagcgtgca acgcctgtgt ctcgctggca cgcgccgccg acggttcatg ccctagctcc
     5401 cccagaacct cctcgatgag ggcgatcatc gactggagat tcgtctctcg ctgattcgca
     5461 aagagtgcct gcgtcatccg gaactcagac taacatcgca cgtggacgat gagtgaggag
     5521 cccgcggaag aacgcccgcc ttcgcgcaag cggctggtga ttctcggtgc ggcggcgctc
     5581 gtcgtcgtga cgctcgctgt gatcgcgatc gtgctgttcg gcaagaaaca cccagcgtcg
     5641 catccgctcg cgctgcgcaa agatggcggc gccgcgacac atgctccgat cgggccgaag
     5701 ctgccgggca ccggcgtcgc gctcacgggg gtcgtcgtcg atggggcggg gctgcccgtc
     5761 gcaggcgcga cgatcgaagc agagctcgag aagggaatgc aagatcgtgc gctcgcgacg
     5821 gccgatgccg gcgtgccttc gcaagatgcg ggcgtcgcca gtccatcggc gccgaccggc
     5881 aatgatggtc gcttcctcgt cgatggcctc gatgccgggc gctaccggct acgcgtaacg
     5941 ggcaccgggt tactcgccgc cgaagtgcgc atggtgccgg tgccgtccga cgagctgcga
     6001 attgtcgtcg cgcgtcaggt cgatattgcc ggagtcgtca ccgacgaggg caagccgatc
     6061 gtaaacgcaa ccgtcggcat tcgcggcgat gcgatcggcg gcacgctcga gaccaagaca
     6121 gacgccgccg gcaagttcaa cgtgccgaat cttccggaag gtcgctacca ggtctacgcg
     6181 tatcgcggcc cactcgctgc tcgcacgatg cgcgtggcgc ggctcggcgc aggtcccttc
     6241 gcgccggtcg agctgcggct cgaagccgga cagaacgtga gcggccgcgt catcgatcgc
     6301 ggcgaaggca cggggctcgt cgcggcgatc gagctgaggc cagttggcga agatcaagcg
     6361 ccgcgctacg cgcgctcgac ggatgacggc agcttcatca tcgaaggcgt gcccaacggc
     6421 cagtggatcg ccgacgcttt ctcgcccggc tacatctcgc ccggtggcgt cgagctgaca
     6481 gcggggcagg gcatcgtgga gcttgccatg gcgcgcggcg cgacgatcga aggccgtgtc
     6541 ctcga
//
LOCUS       DATLBZ010000132        18040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2365501, whole genome shotgun sequence.
ACCESSION   DATLBZ010000132 DATLBZ010000000
VERSION     DATLBZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18040
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365501"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VMZ53_07080"
     CDS             <1..580
                     /locus_tag="VMZ53_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HUS28252.1"
                     /translation="AMGESTSVRAFVREGIWHIWEGIDHILFLLCLLLPAVWRRESAK
                     EVAREVFEIVTAFTLAHSITLVISAVGLVTLPSRFVETAIAASVVAAALNNLVRVVDA
                     RWAGAFALGLLHGFGFSSVLVDLGLPSHELIGTLLGFNLGVELGQAAIVVAVLPVLYA
                     IRKTFAYRALLFGGSGAVVVIATIWTYQRFVV"
     gene            complement(577..3435)
                     /locus_tag="VMZ53_07085"
     CDS             complement(577..3435)
                     /locus_tag="VMZ53_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS28253.1"
                     /translation="MSNHTIMVVDDNATTRRMVKTALTRHGYSVVEAADGKTARELVV
                     SERPSVVLQDLMLPDVDGFDLVGELRELAHADIAIIAFSGFVSKLDEARVSAVGFDDM
                     IAKPIAPSRLLPMIEAHLPAPVAANEQFGNGRRLLVVDDDPLQLKLASFRLGRLGFDV
                     ETAPDGAQALAAIQRRCPDIVVSDVMMPELDGFGLAMALRQDPKLKELPLVLVTSSYI
                     EASDRELARRAGANDLVIRTPELAEVVEILRKTLTRPASSPAIDTKELDNLEKERNRR
                     VVRQLERQVMLNSGLAKRNSALASELTVLSGLGETMLQQRDVVAALDDSLAACFDAGG
                     VTAGALYVLDGKKLRIRAIGEHTWKDEDLQTLFGHQALLDDVLKHGKTVYSEGNRELL
                     ARSHADALLLVPLGNAKGPLGALLMTTRARDIDGSDWAAFGAGVASQISTVLTLSRAY
                     ADVEDAERKAAQHAALLAAVVESAPDTVLQLDTDAKVVFINRTTPPHRPEDVVGKSWV
                     DVLELQHRDKARAAFAMALGGASPEVEIQVTENATIARSYHCRLAPVRQDGKIIGVVI
                     VARDVTDKKQTEMQLMLADRMASVGTLAAGVAHEINNPLAAVIANLDMAMEDVTLLGE
                     TATLPPDLLEELRDARTSADRVREIVRDLKLFSRTQEERYGAVNVEKTIDSTLRMAWN
                     EVRHRAKLVKNYGNVPRVVANESRLGQVLLNLIVNAVQAIPEGNYEANEIRVTTKQDG
                     EHVVIDVSDTGAGIPVDVRGRLFTPFFTTKPVGVGTGLGLAISHRIMSSMNGSITFDT
                     EVGKGTTFHLTLPVAGPTVAPVTKKVPMIAKAVRRGRVLVIDDEESLGQAIRRYLSID
                     HDVEAVTSARVALDMLARGDRYDVILCDLMMPQITGMEVHDTVAKIDQRQAQRIVFVT
                     GGAFTEAARKFFENTPNQRIEKPFDLKTLRLMVNELIV"
     gene            complement(3432..3812)
                     /locus_tag="VMZ53_07090"
     CDS             complement(3432..3812)
                     /locus_tag="VMZ53_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS28254.1"
                     /translation="MGERVLIVDDNPTNLKLVAYLVKAQGYEVATAGDADAALASINE
                     SRPQLILMDLQLPGIDGLELTRRLKADPTTHDIKIVALTAYAMKGDHEKALEAGCDDY
                     VTKPIDTRALPQIIARHLRGAAGE"
     gene            complement(3805..6132)
                     /locus_tag="VMZ53_07095"
     CDS             complement(3805..6132)
                     /locus_tag="VMZ53_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS28255.1"
                     /translation="MRLLVVEDSDDDYEILLRELKRGGVSVDATRVASAGELTRALEQ
                     SWDLMVTDWMLPSFGGLQALEMVAARKVDLPCIVISGTPNEEAAVEALRAGALDFLSK
                     DKPLRFVPAIERAMREAEERRARLEAEKELRFSEQRFRAAFDYAPEAIITYDFALGRI
                     VDVNERVMKLFGYTREQFHAMPVGGLSPPVQPDGRPSAEIAAANLERARRGPISNEWM
                     CRDADGNDFPTEVIWSMLPTSGEPLLRISIVDLRERKRAEELRRRAIELELQNRRIQE
                     ANRLKSEFLANMSHELRTPLNAIIGFAELLHDGQVPTDSPQHTEFLGDILASGRHLLQ
                     LINDVLDLAKVEAGKLDFRPEPIDVVKVLGEIVSITRATASGKRIKVVTEIDPSIRTV
                     TLDPSRFKQVAYNYVSNALKFTPEGGRITIRAKPENDLAFRLEVQDTGVGISATDLGR
                     LFVEFQQLDAGAAKRHQGTGLGLALTRRLVEAQGGTVGVKSTQGEGSVFHAVLPRSAR
                     SSETPVLSRTSMGSRLGQRTVLVVEDEIEDTKLVVDALTSAGYAVETVSNAAEAVAVC
                     RSRTFDAVTLDLVLPDMTGLDLLAMLRAEPHMRTTPVIVVTVIADAKLVAGFAVQDVL
                     RKPLEPHSLLSALERAGVRPDRPGGILVVDDDLGALRLMDATLAQLGFAAITRSNGQA
                     GLEAAEQLKPSAVVLDLMMPGMDGVEFLDRFRRMPAHVRTPVLIWTMKDLTADEHAKL
                     HQSAQGVMSKNGDTPSTVIAQLRALLPLEKEMSGG"
     gene            complement(6136..7113)
                     /locus_tag="VMZ53_07100"
     CDS             complement(6136..7113)
                     /locus_tag="VMZ53_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS28256.1"
                     /translation="MGEVRPDPKRVQLEHEYSTALKDAVGGAGETALSHAYELGRMAA
                     GTGIGIVELAEIHDHAMREVLGQGAPPPEVDQFFAEALSPFEMTHRGFREANERLEAA
                     VRELEAFSYSVSHDLRAPLRTISAFTQALAEDLRHQLDDKAKDHLRRVLAAAARMSDL
                     IDALLELSRISRSQLGRHKVDLSSAAQSVVEELRRRDVTRRLGATIAPDLSVHADGRL
                     MRILLDNLIGNAWKFTTNTSNPHVEIGSEWRDGEQVFFVRDNGAGFDMQYAERLFTPF
                     QRLHSEREYAGTGIGLATVRRIVDRHGGKIWAESVAGGGATFFFTVPAG"
     gene            complement(7106..7732)
                     /locus_tag="VMZ53_07105"
     CDS             complement(7106..7732)
                     /locus_tag="VMZ53_07105"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HUS28257.1"
                     /translation="MTVVEWACSTRALHGQITSGDRGLVLPFDGGALAVVIDGLGHGP
                     EAGKAAECAEAVIRRLVGQPLADVVRECHEACKQTRGVVLSIALFDDRGTMSWLGVGN
                     VEALLIRGGGERTEAVASRGGTVGYLLPPLNPRTLAVQIGDTLVLASDGIKHGFKQEV
                     MAERSVQQIADEVLAQWAKDSDDACALVARYVGVAQDSASIRGGGSNG"
     gene            complement(7729..8127)
                     /locus_tag="VMZ53_07110"
     CDS             complement(7729..8127)
                     /locus_tag="VMZ53_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HUS28258.1"
                     /translation="MSEVRVQIEREADIVLARQAGRQLAAQLGFSSTDQTLIATAISE
                     VARNIVVYAQQGEIVLSRAEEGGRIGVQVIAHDKGPGIDNKDMAMRDGYSTKNSLGLG
                     LPGARRLMDDFALESEIGQGTTVTMKKWKR"
     gene            complement(8120..8485)
                     /locus_tag="VMZ53_07115"
     CDS             complement(8120..8485)
                     /locus_tag="VMZ53_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HUS28259.1"
                     /translation="MPVPILKQGDTLIASVVAALNDDDLVLLRDELAHKIGKVRAKSV
                     VIDVTALDVLDSFAARTLRGIAYTARLRGAETVIVGIQPEVAFAMVQLGLTMEGVPTA
                     LDLEAGLSLLAQRRKLSRE"
     gene            complement(8489..9385)
                     /locus_tag="VMZ53_07120"
     CDS             complement(8489..9385)
                     /locus_tag="VMZ53_07120"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HUS28260.1"
                     /translation="MENVGITDAEMQKRRQFVGLGPEDLARLAAIKPCITKNVDALVE
                     TFFKHLSSIPEAKVLLGYAELTRQARELKRAHLIEMTEGKYDVAYAAQRIRLALLYGR
                     VGLETKVFVGAFQHVLGRAGEIIMEDNPQEGFQRYLSLQKVAFLDIGLHSDVLVHERQ
                     RIIGRQSEAIRELSTPVLQVRERLLLLPLIGVIDTHRAKLITENLLTAIRDARAKVVV
                     MDVTGVATIDSKVANHLVQTVGAARLMGAHTIVSGVTAEVAQSMVALGIEMTPFKTVG
                     DLQGGLELAEHLLGYEVSRSEV"
     gene            complement(9478..10281)
                     /locus_tag="VMZ53_07125"
     CDS             complement(9478..10281)
                     /locus_tag="VMZ53_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS28261.1"
                     /translation="MSLLPVNDTSLYYEDSGPGSTGETIAFSHGLLWGTELFAPQVEA
                     LRDRYRCIAWDHRGQGRSAPEWRHTIDMEQCTYDAIELLDKLGAKQVTFVGLSMGGFV
                     AMRVAARRPDLIRRLVLVETSIGPEARENIGRYRMLTAAYRAIGGRLLEGSVAQIMLG
                     KTILADAARKQDVARFSALMRRRRDVWKAVNGVIDRAPISADELSRIRVPTLVIVGDE
                     DVATPLSKAQQIVAAISGAKLEIVPRAGHSSTVEEPAAVTRLIEQFVRS"
     gene            complement(10262..11125)
                     /locus_tag="VMZ53_07130"
     CDS             complement(10262..11125)
                     /locus_tag="VMZ53_07130"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HUS28262.1"
                     /translation="MRITAEDKVSLAAYWKFFEPHAQAINDALRESLLKLPEWAPVLK
                     SQTPSQLEQQEKEGRERQRGALLDGNWAPYLEDLRKQGITYANMGISFVAWYDIIAIY
                     RDLIRQRLFDAQLSREETVHVSEGMNRAIDIAMSHLGEAYLAAKEQIIAKQQEAIREL
                     SMPVLQVRANLLVIPLVGVLDSTRARQLIESLLASVRDRRAHGVVIDVTGVPIVDTAV
                     ASHLVQACDAAALMGALVVITGISPEMAVTLVGLGAKLPAAETLVDLQEGIEYIEKAL
                     AVQSADVAASG"
     gene            11118..12212
                     /locus_tag="VMZ53_07135"
     CDS             11118..12212
                     /locus_tag="VMZ53_07135"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28263.1"
                     /translation="MRIRVGKVGTRLVYHYQVSDKMRIGELLLLQEKIDPWVLTNTLK
                     EQPYTRQRLVSLLISRAHLDPDDGALILSEQLGYPAAMQRHLERRDPSLLTLVPPQLG
                     STWVVLPLGYAKTGALIVVSRDPTPILSAALEHIVRKSVLLAVTPAVQLERLVRAAYG
                     GAGTPDEPLPHSPPTLSDIGHVQLEDATPLPVRRARTVSYMFDGPPQLPVRAPHHVAP
                     IETTLREVDNAITAHAAERHVMAYAAKRWRTALLVRLERDFAIGVRGHGPELEHPEQV
                     RVPLREPSLVSAAAGSRRATQESPSSDTQTHLRVLLGSPATPAAAPIIVGTQVEAVLA
                     VGDPIDHATLAELDRLVDALGAAYSRFARG"
     gene            12276..13919
                     /locus_tag="VMZ53_07140"
     CDS             12276..13919
                     /locus_tag="VMZ53_07140"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS28264.1"
                     /translation="MSTEKKTKGMCIATRCTSVDQFVQMFARFVDEESFFVSTHNTRP
                     AGLETSFSVQLADGTPVLRGLCTVLAAWTDANNVFKTPGVRLGIKRLTASSMPVFERL
                     LVTRSAPTPPPIPKRASDRDKTTELKREMEDVKTSVVPPKLPPPPPVPELEPDDEPEI
                     ETRTPGSDYILPANPLMNLSDASLDGFVDCTLYEETGNFFPAEDNLDDPMLTDDVVPP
                     PVLATRRPTTAPVVLPANPLAPEVTISSVVAPLPGDSIAALLPTEPVASNSVVTPLPL
                     STSVVTPLPSYGPMPTIPPPMSTMPPMRETPIPVGERIDLAPRARRKKPPMWMLGGGF
                     GVIAIATLIIVISGKSSSGSSATKPAKQIKPEVVAQVELPKPEQPVVEEATDTAATTE
                     AKTGVLEDPDGPPMVGDGPCKLTVATTPAGSMIALDGKELAPSPLTIATSCEKHKIGI
                     AHARYQSATKMITLVEGTPGAIDVSLNRPTHAVSITSLPQGATIFIDGRRAGTTPTVV
                     NVVGFQRLKIEVKKTGFVAQSVSLYSKLAQDKVSVRLKK"
     gene            complement(13931..14524)
                     /locus_tag="VMZ53_07145"
     CDS             complement(13931..14524)
                     /locus_tag="VMZ53_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28265.1"
                     /translation="MTKLVCLLALVLAVACSKSKPAEQGAGSSTTTTTPPPVADAAQA
                     APADAADTGSGAPAMDGSGSSAAAGSGAGSSADDGPQSDFDKLSHDDKIKFMKTKVMP
                     AMKPLFQKFDAKEYANFGCKTCHGKDPQKAKYKMPNPDLKALDFEAIKAGKEDPKMVD
                     FMSKKVKPEMAKVFGVPEMTQTEPKGFGCLDCHTMKK"
     gene            14936..16333
                     /locus_tag="VMZ53_07150"
     CDS             14936..16333
                     /locus_tag="VMZ53_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS28266.1"
                     /translation="MCVAAEDAPLRIGHHSALSGTNQALGTNMKLGIELAFDEANKAG
                     GIRGRQLQLEFRDDQYDPASAESAARALVDATAMTGEAPHCPTTSLKPPIGSTTSDAP
                     ALSDTRLVRGPNAVLAMIGNVGTPTAVRAAPIVVETGTVFFGPFTGAGTILRDTQCGE
                     CAKYIFNVRASYAQEARATLEYFKRKAVGGSATDHKYLISFDQNDTYGQAGYDGLVNA
                     YKEYIGAFPGTADATNPIVRFRYVRNDDTSVPAQAVAATNYLNTLLSTNSGNLIVGVM
                     MTDTYGSGTEFIKRVRDWQYGSDQTNNQNTRLTIYFSNVSFVGPNTLAERLVALGTYN
                     TPSGPKAYADGVVVSQVVPNYQNDTSDVVVKYNQLIKDAGKLPTFTSLEGYIAARIFI
                     AGLERHKGPFTPDSLVDGFETLPDLGLGLGASSGFKPDNHQYSQSVWGTVIQADGTFK
                     NLYFWSKGIPISFYD"
     gene            16391..17977
                     /locus_tag="VMZ53_07155"
     CDS             16391..17977
                     /locus_tag="VMZ53_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28267.1"
                     /translation="MLHRQIARGGMATIHIARLMGDEGFSRIVAAKRLLPEFAEDTEF
                     VAMFMDEARIASKVHHPNVVPVLDLVTTTDEVMLVQEYVHGVPLHVLLRTAHEAGKKI
                     PVKIAVSIAAQVLAGLHAAHTTLDEMGAPLNIVHRDVSPQNVMVAVDGSARLLDFGVA
                     KATMGAHITREGTFKGKLAYSAPEQVRGKPCQQSDIYALSVVLWELLVGHRMHGSSQG
                     EAELVATIMNGNIPPVTEALSNEADWGAIDSDEWKMLSAIETIVAKGLDVDLAKRYDT
                     AADMETDLRKAVTVASSNEVSAWVKSLGKDFIESRDKVIAAEEQSWRRTASTTGVRRT
                     PLPGSLPRPSTISEVSQPQLPLAPKEVRRTRMIIGVLGALVILLAVGVVVAMQTGDSN
                     KHEAQAAPAPTQPTAVETEKQAPPPAAMPTEKQEPKHETATAPPQPTQPTETAATEAA
                     TTETTASTETTETATTEKPTTTTTRKWGRNRVVKKAEPEKKDPPPVVTPAVTTEKPAN
                     DCSPPYYYEGQKKVFKPNCL"
BASE COUNT         3165 a         6013 c         5655 g         3207 t
ORIGIN      
        1 ggcgatgggc gagtcgacgt cggtgcgcgc gtttgtccgc gagggcatct ggcacatctg
       61 ggagggcatc gaccacattc tgtttctgct gtgtctgctg ctgcctgcgg tgtggcggcg
      121 cgagtccgcg aaggaagtcg cgcgcgaggt cttcgagatc gtcacggcat tcacgctcgc
      181 gcattcgatc acgctcgtga tctcggcagt cgggctcgtc acgctgccgt cgcgctttgt
      241 cgagaccgcg attgcggcgt cggtggttgc ggccgcactg aacaacctcg tgcgcgtcgt
      301 cgatgcgcgg tgggcgggtg cgttcgcgct cgggctgttg catggctttg gcttttcgag
      361 cgtgcttgtc gatttggggc tgccgtcgca cgagctcatc ggcacgctgc tcggcttcaa
      421 cctcggcgtc gagctcggtc aggctgccat cgtcgtggcc gtgctgccgg tgctgtatgc
      481 gattcgcaag acgttcgcgt atcgcgcgct gctgtttggc ggctccggcg cggtcgtcgt
      541 catcgcgacg atttggacgt accagcgctt cgtcgtttag acgatcagct cgttgaccat
      601 cagacgcagc gtcttgagat cgaacggctt ctcgatgcgc tggttgggcg tgttctcgaa
      661 gaacttgcga gcggcttcgg tgaacgcacc gcccgtgacg aacacgatcc gttgggcttg
      721 ccgctggtcg atcttcgcga cggtgtcgtg aacttccatg cccgtgatct gcggcatcat
      781 caggtcgcag aggatcacgt cgtagcggtc gccgcgggca agcatgtcga gtgcgacgcg
      841 cgcgctggtg acggcctcga cgtcgtggtc gatggagaga tagcggcgaa tcgcttggcc
      901 gagcgactct tcatcgtcga tgacgagcac gcgaccgcgg cggaccgcct ttgcgatcat
      961 cggcactttc ttcgtgaccg gcgcaaccgt cgggcccgcg accggcagcg tgaggtggaa
     1021 cgtcgtgccc ttgccgacct cggtgtcgaa cgtgatcgac ccgttcatcg acgacatgat
     1081 ccgatgcgag atcgcgaggc cgaggccggt gccgacaccg acgggcttcg tcgtgaagaa
     1141 cggcgtgaac agccgcccgc gcacgtcgac cgggatgccc gcgccggtgt cgctgacgtc
     1201 gatcacgacg tgctcgccgt cttgcttcgt ggtcacgcga atctcgttgg cttcgtagtt
     1261 gccctcggga atcgcctgca ccgcattgac gatcaggttc agcagtactt ggccgaggcg
     1321 cgattcgttc gcgacgacgc gcggcacgtt gccgtaattt ttgaccagct tcgcgcggtg
     1381 gcggacctcg ttccacgcca tgcgcagcgt cgaatcgatc gtcttctcga cgttcaccgc
     1441 gccgtagcgc tcttcttgcg tccgagagaa cagcttgagg tcgcgcacga tctcgcgtac
     1501 gcgatcggcg gaggtccgcg catcgcgcag ctcctcgagg aggtctggcg gcagcgttgc
     1561 ggtctcgccg aggagtgtga cgtcctccat cgccatatcg aggttcgcaa tgacggcggc
     1621 gagcgggttg ttgatctcgt gcgcgacacc cgccgcgagc gtaccgacgg acgccatgcg
     1681 atcggcgagc atcagctgca tctcggtctg cttcttgtcc gtcacgtcgc gcgcaacgat
     1741 cacgacgccg atgatcttgc cgtcctggcg caccggcgcg aggcggcagt gataggagcg
     1801 tgcgatcgtc gcgttctcgg tgacttggat ctcgacttcc ggcgacgcac caccgagcgc
     1861 catcgcgaac gcggcgcgcg ctttgtcgcg atgctgcagc tcgagcacgt cgacccacga
     1921 cttgccaacg acgtcctcgg gcctgtgcgg cggcgtcgtg cgattgatga agacgacctt
     1981 ggcgtcggtg tcgagctgca gcacggtgtc cggcgcactc tcgacgacgg cggcgaggag
     2041 tgcggcatgc tgcgcggcct tgcgctcggc atcctcgaca tccgcgtacg cgcgcgagag
     2101 cgtgagcacc gtgctgatct gcgaggcgac gcccgcgccg aacgcggccc agtcggagcc
     2161 gtcgatatcg cgcgcgcgcg tcgtcatgag caacgcgccg agcggcccct tcgcattgcc
     2221 gagcggcacg agcagcagcg cgtcggcatg gctccgcgcg agaagctcgc gattgccctc
     2281 gctgtacacg gtcttgccgt gcttcagcac gtcgtcgagc agcgcctgat gaccaaacag
     2341 cgtctggaga tcctcgtcct tccaggtgtg ctcaccgatc gcgcgaatgc gtagcttctt
     2401 gccgtcgagg acgtagagcg cacctgccgt gacgccgccg gcgtcgaaac acgctgccag
     2461 tgaatcgtcg agcgcggcga cgacatctcg ttgctggagc atcgtctcgc cgaggcccga
     2521 gagcacggtc agctccgacg cgagtgccga gttgcgcttc gcaaggccgc tgttgagcat
     2581 cacctgacgc tcgagctggc ggacgacgcg acggttgcgt tccttctcga gattatcgag
     2641 ctctttggtg tcgatcgctg gcgaagatgc aggcctcgtc agcgtcttgc ggaggatctc
     2701 gacgacctcc gcgagctctg gcgtgcgaat cacgagatcg tttgcgccgg cgcgtcgtgc
     2761 gagctcgcga tccgatgcct cgatgtagct cgacgtgacg agcacgagcg gcagttcctt
     2821 cagcttcgga tcctgccgca gcgccatcgc gagcccgaat ccgtcgagct ccggcatcat
     2881 cacgtcggat acaacgatgt ccggacaccg tcgctggatc gccgcgagcg cctgtgcgcc
     2941 gtccggtgca gtctcgacgt cgaagccgag gcggccgagc ctgaacgatg cgagcttgag
     3001 ctgcagcgga tcgtcgtcga cgacgagcag ccgcctgccg ttgccgaact gctcgttggc
     3061 cgccacgggc gccgggagat gcgcttcgat catcggcaag agccgcgacg gcgcgatcgg
     3121 cttcgcgatc atgtcatcga atccgacggc cgagacgcgc gcctcgtcga gcttcgagac
     3181 gaatcctgag aacgcgatga tcgcgatgtc ggcgtgcgcc agctcgcgca actcgccgac
     3241 gagatcgaag ccatcgacat cggggagcat gagatcctgc agcacgacac tcggccgctc
     3301 actcacgacg agctcgcgcg cagtcttgcc atctgccgct tcgacgacgg agtagccatg
     3361 ccgcgtgagc gcggtcttca ccatccggcg agtcgtcgca ttgtcgtcga cgaccatgat
     3421 ggtgtgattg ctcattcgcc cgcagctccg cgtaggtggc gcgcaatgat ctgcggcaac
     3481 gctcgagtgt cgatcggttt ggtcacgtag tcatcgcaac ctgcctcgag cgccttctcg
     3541 tggtcgccct tcatcgcata cgccgtgagc gcgacgatct tgatgtcgtg cgtagtcgga
     3601 tccgccttga gccgacgcgt cagctccaga ccgtcgatac caggcagctg aaggtccatg
     3661 aggatgagct gcggtcgact ctcgttgatc gacgcgagcg cggcatcggc atcacccgcc
     3721 gtggcgactt cgtacccttg tgccttcacg agataggcga cgagctttag gttcgtcgga
     3781 ttgtcgtcga cgatcagtac tcgctcaccc accgctcatc tccttctcca acggcagtag
     3841 cgcgcgcagc tgcgcgatca ccgtcgatgg cgtgtcgccg ttcttcgaca tcacaccctg
     3901 cgcggactga tggagcttgg cgtgctcgtc cgcggttaga tctttcatcg tccagatcag
     3961 caccggcgtc cgcacgtgcg cgggcatgcg ccgaaaccga tcgagaaact ccacgccgtc
     4021 catcccaggc atcatcaagt cgagcaccac cgcgcttggc ttgagctgct ccgccgcttc
     4081 gagccctgct tgaccattcg atcgcgtgat cgcggcgaat cccagctgcg cgagcgtcgc
     4141 gtccatcaag cgcagcgccc ccagatcatc gtcgacgacg aggatgccgc ccggccgatc
     4201 cggtcgcacg ccagcacgct cgagtgcgga gagcagcgag tgcggctcca gcggcttgcg
     4261 caacacgtct tgcacggcga acccggcgac gagctttgca tccgcgatca cggtcacgac
     4321 gatcaccggc gtcgttcgca tatgcggctc ggcacgcagc attgcgagca gatcaagccc
     4381 ggtcatatcg ggcagcacga ggtcgagcgt caccgcatcg aacgtgcgac tccggcagac
     4441 cgccacggct tccgccgcgt tcgacacggt ttccaccgca tagccggcgg acgtcagcgc
     4501 atcgacgacg agcttggtgt cctcgatttc gtcttcgacg acgagcacgg tgcgctgccc
     4561 gagccgtgag cccatcgacg tgcgcgatag aaccggcgtt tcactcgacc gtgcgctgcg
     4621 cggaagcacc gcgtgaaaca cgctgccctc gccctgcgtc gatttcacac cgacggtgcc
     4681 gccttgcgct tcgacaagcc ggcgcgtgag cgcgagcccg aggcctgtgc cctgatgacg
     4741 cttcgctgcg cctgcatcga gctgctggaa ctcgacgaac agccggccga gatccgtggc
     4801 cgagatgccg acgcctgtat cctgcacttc gaggcggaac gccaagtcgt tctcgggctt
     4861 cgcgcggatc gtgatgcgtc caccctcggg cgtgaacttg agcgcgttcg agacgtagtt
     4921 gtatgcgacc tgcttgaagc ggctcggatc gagcgtcacc gtgcgaatgc tcggatcgat
     4981 ctcggtcacg accttgatcc gcttccccga cgcggttgcg cgagtgatcg acacgatctc
     5041 gccgaggact ttgacgacgt cgatcggctc gggacggaag tcgagcttgc ccgcctcgac
     5101 ctttgcgaga tcgagcacgt cgttgatgag ctgaagcagg tgacgaccac tcgccagaat
     5161 gtcgccgagg aactcggtgt gctgcggtga atccgtcggc acctgcccgt catgcagcag
     5221 ctccgcgaag ccgatgatcg cattcagcgg cgtgcgcagc tcgtgcgaca tgttcgcgag
     5281 gaactcgctc ttcaagcggt tcgcttcttg gatgcgtcga ttctgcagct cgagctcgat
     5341 cgcgcggcgc cgcagctcct cggcacgttt cctctcgcgc aggtcgacga tgctgatgcg
     5401 gagcagcggc tcgcccgacg tcggcaacat cgaccagatc acctcggtcg ggaaatcgtt
     5461 gccatcggca tcgcggcaca tccactcgtt cgagatcggt ccgcgccgtg cgcgctccaa
     5521 gttcgcagcc gcaatctctg ccgacgggcg accgtctggc tgtacgggcg ggctcaaacc
     5581 gccgacgggc atcgcgtgga attgctcgcg cgtgtagccg aacagcttca tcacgcgctc
     5641 gttgacgtcg acgatgcggc cgagcgcgaa gtcgtaggtg atgatcgctt cgggcgcgta
     5701 atcgaacgcg gcgcggaagc gctgctcgct gaatcgcagc tctttctcgg cctcgaggcg
     5761 cgcgcgcctc tcttctgctt cgcgcatcgc gcgctcgatc gccggcacga atcggagcgg
     5821 cttgtccttc gacaagaagt cgagcgcccc agcgcgcagc gcttcgacgg ccgcttcctc
     5881 attcggcgtt cccgagatga cgatgcacgg caggtcgacc ttgcgcgccg ccaccatctc
     5941 gagtgcctgc aaaccgccga agctcggcag catccaatcg gtgaccatga ggtcccacga
     6001 ctgttccaac gctcgcgtga gctcgcccgc cgatgcgacg cgcgttgcgt cgacagagac
     6061 cccgccgcgt ttcagctcgc gcagcaggat ctcgtagtcg tcatcggagt cttcaacgac
     6121 gagcaatctc aaggctcacc ccgccggcac cgtgaagaag aaggttgcgc cgccacctgc
     6181 aacgctctcc gcccagatct tgccgccgtg gcggtcgacg atccggcgaa ccgtcgcgag
     6241 cccgataccg gttcccgcgt actcgcgctc gctgtgcagg cgctggaagg gcgtgaacag
     6301 acgctcggcg tactgcatgt cgaagccggc accgttgtcg cgcacgaaga agacttgctc
     6361 gccgtcgcgc cactcgctgc cgatctcgac gtgtgggttc gacgtgttcg tcgtgaactt
     6421 ccacgcgttg ccgatcaggt tatcgagaag gatgcgcatg aggcggccat cggcgtggac
     6481 gctgaggtcc ggtgcgatcg tggcgcccag cctgcgcgtg acgtcgcgcc tgcgcagctc
     6541 ttcgacgaca gactgcgccg ccgacgagag atcgaccttg tggcggccca gctgcgaacg
     6601 actgatgcgt gagagctcga gcagtgcgtc gatcagatcg ctcatgcgcg ctgcggccgc
     6661 gagcacgcga cgaaggtgat ccttcgcctt gtcgtcgagc tgatggcgga gatcttcggc
     6721 gagcgcctgc gtgaacgcgc tgatcgtgcg tagcggcgcg cgaaggtcat gcgacaccga
     6781 gtacgagaac gcttcgagct cacgcacggc cgcttcgagc cgctcgttcg cctcgcggaa
     6841 tccgcggtgc gtcatctcga acggagacag cgcctccgcg aagaattgat cgacctccgg
     6901 cggcggcgcg ccctgaccga gtacctcgcg catagcgtga tcgtggatct cggcgagctc
     6961 gacgataccg atgccagtgc cggcggccat gcgaccgagc tcgtacgcgt gcgagagcgc
     7021 ggtctcgccc gcgccaccga cggcatcctt gagcgccgtc gaatattcat gctcgagctg
     7081 aacacgcttg ggatcgggcc ttacttcacc cattactgcc tccgccgcgt atggatgcgc
     7141 tgtcctgcgc aacgccaaca tagcgtgcga cgagagcgca ggcgtcatcg gaatctttcg
     7201 cccactgagc caggacctcg tcagcaattt gctggacgga acgctcggcc atgacttcct
     7261 gcttgaagcc atgcttgatg ccatcgctcg cgagcacgag cgtgtccccg atttgcacgg
     7321 cgagcgtgcg cgggttcagc ggaggaagca gatagccgac cgtgcctcca cgcgaggcca
     7381 ccgcctccgt acgctccccg ccgccacgaa tgaggagcgc ttcgacgtta cccacgccga
     7441 gccacgacat cgtcccgcga tcatcgaaca acgcgatgct gagcacgacg ccgcgcgtct
     7501 gcttacaggc ctcgtgacac tcgcgcacca catcggccag gggttgcccg accaagcgac
     7561 ggatcacggc ctcggcgcat tccgccgctt tcccggcttc tggtccgtga ccgagaccat
     7621 cgatcacgac tgcgagcgcg ccgccgtcga aagggagcac gagtccgcga tcgcccgagg
     7681 tgatttgccc gtgcagcgcg cgcgttgaac atgcccactc gacgaccgtc atcgcttcca
     7741 cttcttcatc gtgacggtag tgccctggcc gatctcgctc tcgagcgcga agtcatccat
     7801 caagcgacgc gcgcctggta ggccgaggcc gaggctgttc ttcgtcgaat agccgtcgcg
     7861 catcgccatg tctttgttgt cgatgccggg gcctttatcg tgggcgatca cctggacgcc
     7921 gatacgccca ccctcttccg cgcgactgag gacgatctcg ccctgctggg catagacgac
     7981 gatgttgcgc gcgacttcgg agatcgcggt tgctatcagc gtctgatcgg tgctcgagaa
     8041 tccgagttgc gcagcgagct gccgtccggc ttgcctcgcg agcacgatat cggcttcgcg
     8101 ttcgatctgg acgcgtactt cactcacgcg acaacttcct ccgctgggca agcagcgaaa
     8161 ggcccgcctc tagatcgagc gcggtcggta cgccctccat ggtgaggccg agctgcacca
     8221 tggcgaacgc gacttctggc tggatgccaa cgatcaccgt ctcggctccg cgcaaccggg
     8281 cggtatacgc gatgccgcgc agcgtgcgtg ccgcgaacga atcgagaaca tcgagcgcgg
     8341 taacgtcgat cacgacactc ttcgcgcgca ccttgccgat cttgtgcgcg agctcgtcac
     8401 ggagcagaac gagatcgtcg tcgttgagtg cagcgacgac cgatgcgatc agcgtgtcac
     8461 cctgtttgag aatcggtacc ggcattgctc atacctccga ccgcgagact tcgtagccga
     8521 gcagatgctc tgccagctct agcccgccct gcagatcgcc cacagtcttg aacggcgtca
     8581 tctcgatccc gagcgcgacc atcgattgcg cgacctcggc ggtgacgccg ctgacgatcg
     8641 tgtgcgcacc catcaagcga gctgcgccga ccgtctgcac gagatggttt gcgaccttcg
     8701 aatcgatcgt cgcaacaccc gtcacgtcca tcacgaccac cttcgcgcga gcatcgcgaa
     8761 tggcggtcaa aagattttcg gtgatcagct tcgcgcggtg cgtatcgatc acgccaatga
     8821 gcggcaacaa cagcagacgc tcgcgaactt ggaggaccgg cgtcgacagc tcgcggatcg
     8881 cttccgattg gcggccgatg atccgctgcc gctcgtgaac gagcacgtcc gagtgcaggc
     8941 cgatgtcgag gaacgcgacc ttctgcagcg acaggtagcg ctggaagcct tcctgcgggt
     9001 tgtcctccat gatgatctcg cccgcgcgac cgagcacgtg ttggaacgcg ccgacgaaga
     9061 ccttcgtctc gagcccgacg cgtccgtaga gcagcgcgag cctgatccgc tgcgccgcgt
     9121 acgcgacgtc gtacttgccc tcggtcatct cgatcaggtg cgcgcgcttg agctcgcgag
     9181 cctgacgcgt cagctctgcg tagccgagca acaccttcgc ttcagggatc gaagacaggt
     9241 gcttgaagaa agtttcgacc aacgcatcga cgttcttcgt gatgcacggc ttgatcgctg
     9301 cgagccgcgc gagatcctcg ggaccgaggc cgacaaactg ccggcgtttc tgcatttccg
     9361 cgtcggtgat cccgacgttt tccatgacgg tttgttcagt gactggtaag ccgcgtactg
     9421 acgacacccg atcggtttta caggaggatc acccggtccg tcgcgtgtta acggggctta
     9481 gctgcgtacg aactgttcga tgagccgcgt tacagccgca ggctcttcca ccgtcgacga
     9541 atggccggct cgcggcacga tctcgagctt cgcgccggag atggccgcaa caatttgttg
     9601 cgccttgctg agcggtgttg ccacatcttc gtcgcctacg atcacgagcg tcgggacgcg
     9661 aatccgcgac agctcatcgg cgctgatcgg tgcgcgatcg atcacgccgt tgacagcttt
     9721 ccagacgtcg cgacgccgcc tcatgagcgc cgagaaccgc gcgacatctt gcttgcgcgc
     9781 agcatccgcc aagatcgttt tgccgagcat gatctgcgcg acgctgccct cgagcagccg
     9841 gccaccgatc gcacgatacg ccgcggtcag catgcgatag cggccgatgt tctcgcgcgc
     9901 ctccgggccg atcgatgtct cgacgagcac gaggcggcga atcagatcgg gtcggcgcgc
     9961 cgccacgcgc atcgcgacga agccacccat cgagaggccg acaaacgtca cctgcttcgc
    10021 gccgagcttg tcgagcagct cgatcgcatc gtaggtgcac tgctccatat cgatcgtgtg
    10081 tcgccactcg ggcgcgctcc gaccctgccc gcggtgatcc catgcgatgc agcgatagcg
    10141 atcgcgcagc gcttcgacct gcggcgcgaa cagctcggta ccccagagca gaccgtgcga
    10201 aaacgcgatg gtctctcccg tcgagccggg accagagtcc tcgtaataca gcgacgtatc
    10261 gttaaccgga agcagcgaca tcggcggact gtaccgccag tgccttctcg atgtactcga
    10321 tgccttcctg cagatcgacg agcgtctctg ccgcgggcag cttcgcgccg agaccaacga
    10381 gcgtcaccgc catctccggc gagatacccg tgatgacgac gagcgcaccc atcagcgccg
    10441 ctgcgtcaca cgcctgcacg aggtggctcg cgaccgcggt atcgacgatc ggcacgcccg
    10501 tcacatcgat cacgacgcca tgcgcgcggc gatcgcgaac cgatgcgagc aagctctcga
    10561 tcaactggcg ggcgcgcgtc gaatcgagaa caccgacgag cgggatgacg agcaggtttg
    10621 ctcggacctg cagcacgggc atcgacagct cgcgaatcgc ctcttgctgc ttcgcgatga
    10681 tctgctcttt cgcggcgaga taggcctcgc cgaggtggct catcgcgatg tcgatcgcac
    10741 ggttcatgcc ctcgctgacg tgcacggtct cttcgcgcga cagctgcgca tcgaacagac
    10801 gctggcggat caggtcgcga tagatcgcga tgatgtcgta ccaggcgacg aacgagatgc
    10861 ccatgttcgc gtaggtgatg ccctgcttgc gaaggtcctc gagatacggc gcccaattgc
    10921 cgtcgagcaa cgcgccgcgc tgacgctcac ggccttcctt ctcctgctgc tccaactgcg
    10981 acggagtctg gctcttcaga accggcgccc actcgggcag cttcaacagt gattcacgca
    11041 gcgcatcgtt gatggcctgc gcgtgcggct cgaagaattt ccagtacgca gcgagggaaa
    11101 ccttgtcctc ggcggtgatg cgcattcgcg ttggcaaggt cggcactcga ttggtgtatc
    11161 actaccaggt gtcggacaaa atgcggatcg gcgagctgct cctactccag gagaagatcg
    11221 atccgtgggt tctgacgaac acgctcaaag agcagcccta cacgcgtcag cgtttggtct
    11281 ccctcctgat cagtcgcgcg catctcgatc ccgatgacgg cgcgctgatc ctctccgagc
    11341 agctcggcta tccggccgcg atgcagcgtc acctcgagcg tcgcgacccg tcgctactca
    11401 cgctcgtgcc accgcagctc ggctcgacgt gggtcgtgct gccgctcggg tacgcgaaga
    11461 cgggcgcact gatcgtggtc tctcgcgatc cgacgccgat cctgtccgct gcgctcgagc
    11521 acatcgtgcg caagtcagtg ctgctcgcgg tgacgccggc ggttcagctc gagcgactcg
    11581 tgcgcgctgc gtacggcgga gccggcacgc ccgacgagcc gctgccgcat tcgccgccga
    11641 cgctctcgga catcggccac gtacagctcg aagacgccac gccgctgccg gttcgtcgcg
    11701 cccgcaccgt ctcatacatg ttcgacggtc caccgcagct gcccgtgcgc gcgccgcatc
    11761 acgtcgctcc gatcgagacg acgctgcggg aagtggacaa cgcgatcacc gcgcacgccg
    11821 ccgagcgtca cgtgatggcg tacgcggcga agcgctggcg caccgcgctg ctcgttcgtc
    11881 tcgagcgcga ttttgcgatc ggtgttcgcg gtcacggtcc cgagctcgag catcccgaac
    11941 aggtgcgcgt cccgctgcgc gagccgtcgc tcgtgtctgc cgccgcggga tctcgccgcg
    12001 caacgcagga atctccgagc agcgacacgc agactcatct ccgagtcctc ctcggatctc
    12061 cggccactcc cgcggctgcc ccgatcatcg tcgggacgca ggtcgaggcc gtgctcgccg
    12121 tcggagatcc gatcgatcat gcgacgttgg ccgagctcga tcggttggtc gacgctctcg
    12181 gcgcagcgta ctcccggttc gcgcgcggct gatacctgct cccacatcag accctggtaa
    12241 ccccgcgggc gaccatggct agatcgaggg tgggcgtgtc gacggagaag aagacaaagg
    12301 ggatgtgcat cgcgacgcgc tgtacgagcg tcgatcagtt cgttcagatg ttcgctcgct
    12361 tcgtcgatga agaatcgttc ttcgtctcga cgcacaacac gcggcccgca gggctcgaga
    12421 cgtcgttctc ggtccagctc gccgacggaa cgccggtgct gcgcggactc tgcaccgtgc
    12481 tcgccgcgtg gactgacgcg aacaacgtgt tcaagacgcc aggcgttcgc ctcggcatca
    12541 agcgcctcac ggcgtcgagc atgccggtgt tcgagcgtct gctcgtcacc cgctccgcgc
    12601 cgacgccacc accaattccg aagagggcat cggaccgcga caagacgacc gagctcaagc
    12661 gcgagatgga agacgtcaag acgagcgtcg ttcctccgaa gcttcctcct ccgccgcctg
    12721 taccggagct cgaaccggac gacgagcccg agatcgaaac gcgcacgcca ggctccgact
    12781 acatcctgcc ggcaaacccg ctcatgaacc tcagcgacgc atcactcgac ggcttcgtcg
    12841 actgcacgct ctacgaggag accgggaact tcttcccggc cgaggacaac ctcgacgatc
    12901 cgatgctgac cgacgacgtc gtgccgccgc ccgtgctcgc gacgcgccgc ccgacgacgg
    12961 cgccggtggt gctgcccgcg aacccgctcg cgccggaagt cacgatctcg tcggttgtcg
    13021 cgccgctgcc cggcgactcg atcgccgcct tgctccccac cgagccggtc gcgagcaact
    13081 ctgtcgtcac accgctgccg ctgagcacgt ccgtcgtgac gcccctgccg tcgtacgggc
    13141 cgatgccgac gattccgccg cccatgtcga cgatgccgcc gatgcgcgag acgcccatcc
    13201 ctgtgggaga gcgcatcgac ctcgcgccgc gagcacgtcg caagaagccc ccgatgtgga
    13261 tgctcggcgg cggcttcggc gtgatcgcga tcgcgacgct catcatcgtg atcagcggca
    13321 agagctcgtc gggctctagc gcgacgaagc cggccaagca gatcaagccc gaggtcgtcg
    13381 cgcaggtcga gctgccgaag cccgagcagc ctgtcgtcga ggaagcgacc gacacggccg
    13441 ccaccactga ggcgaagact ggcgtcctcg aggatcccga cgggccgccg atggtcggcg
    13501 acggaccgtg caagctcacc gtcgcgacaa cacctgcggg ctcgatgatc gcgctcgacg
    13561 gcaaagagct cgctccctcg ccgctgacga tcgcgacgtc atgcgagaag cacaagatcg
    13621 gcatcgcgca tgcgcgctac caatcggcga cgaagatgat cacgctcgtc gaaggcacac
    13681 cgggtgcgat cgacgtcagc ttgaatcgcc cgacgcacgc ggtttcgatc acgagccttc
    13741 cgcagggcgc gacgatcttc atcgacggac gccgagcagg cacgacgccg accgtcgtca
    13801 acgtcgtcgg atttcagcgc ctcaagatcg aggtgaagaa gacgggcttc gtcgcgcaga
    13861 gcgtctcgct gtacagcaag ctcgcgcagg acaaggtcag cgttcgcctg aagaagtagt
    13921 agctcggtag ctacttcttc atcgtgtggc agtcgaggca gccaaagcct tttggctcgg
    13981 tctgcgtcat ctcgggcacg ccgaagacct tcgccatctc gggcttcacc ttcttcgaca
    14041 tgaagtcgac catcttcggg tcttccttgc ccgctttgat cgcttcgaag tcgagcgcct
    14101 tcagatccgg gttcggcatc ttgtacttcg ccttctgcgg gtccttgccg tggcaggtct
    14161 tgcaaccgaa attcgcgtac tccttcgcgt cgaatttctg gaacagcggc ttcatcgccg
    14221 gcatgacctt cgtcttcatg aacttgatct tgtcgtcgtg agacaactta tcgaagtccg
    14281 actgcggacc gtcgtcggcc gacgagccgg cgcccgagcc tgcggcagcc gacgagccac
    14341 ttccgtccat cgcaggagcg ccagaaccgg tatcggccgc gtcggcagga gcggcttggg
    14401 cagcgtccgc aacaggcggc ggcgtcgtgg ttgtcgtcga cgaaccagcg ccttgctcag
    14461 cgggcttgga cttcgagcac gcgaccgcga gcacgagggc tagaaggcaa acgagcttgg
    14521 tcatagaggc ccgcatccta ggggcgatgg cctatgccat caagatctca cgcccgtaaa
    14581 ccgagcgctc gcgcacaccc cttttccgac ggtgagcgat cgcgcacgga gatcggaaca
    14641 cacattgttg acactcaaaa tggttcgtta gtcgcctcac gttcctcgtc gaggaagaac
    14701 gacaaagctg cacgaactca gtgttctggc cggtgccgat gtgagcctca agctcccttg
    14761 cgcactgtca acgaaaactt tcagtatggg cactatgggt tcttccctcg ctcgaatcgt
    14821 tggaacaaca gcgattgtct ttgcgggttg ctcgaccgag ttcacgcctc agccgtgtgc
    14881 cgtcgacaac gactgcggca cgggtttagt ttgcgagctt cgcgatcaaa atcccgtgtg
    14941 tgttgctgct gaggacgcgc cgctcagaat cggccaccac agcgctctca gcggcactaa
    15001 ccaagccctc ggcacgaaca tgaagctcgg catcgagctc gcgttcgacg aggccaacaa
    15061 agccggcggt attcgcggac gccagctgca gctcgaattc cgcgatgacc agtacgatcc
    15121 ggcgagcgcg gaatccgcag cgcgcgcact agtcgacgca accgcgatga cgggtgaagc
    15181 gccgcattgc ccgacgacgt cgctcaagcc gccgatcggc tcgacgacgt ccgatgcgcc
    15241 ggcgctttcg gacacgcgcc tcgtgcgagg acccaatgcc gtgctcgcca tgatcggcaa
    15301 cgtcggtacg ccgaccgctg ttcgtgcagc acccattgtc gtcgagacag gcaccgtgtt
    15361 cttcggcccg ttcacgggtg caggcacgat tcttcgcgac acgcagtgcg gcgagtgcgc
    15421 gaagtacatc ttcaacgttc gcgcgagcta cgcacaggaa gcacgcgcga cgctcgagta
    15481 cttcaagcgt aaggccgtcg gcggcagcgc aaccgatcac aagtatctca tctcgttcga
    15541 ccagaacgat acgtacggcc aggccggtta cgacggtctc gtcaacgcat acaaggagta
    15601 catcggcgcg tttccgggaa ccgccgatgc gacaaacccg attgttcggt tccgctacgt
    15661 gcgcaacgac gacacgagcg tgccggcgca agcagtcgcc gcgacgaact acctcaacac
    15721 gctgctttcg acgaacagcg gcaatctcat cgtcggcgtg atgatgacgg acacgtacgg
    15781 ctcgggcacc gagttcatca agcgcgtgcg cgactggcag tacggctccg accagacgaa
    15841 caaccaaaac acgcgtctga cgatctactt cagcaacgtg tcgttcgtcg gtccgaacac
    15901 gctcgccgag cgtctcgttg cactcggcac gtacaacacg ccgagcggtc ccaaggcgta
    15961 cgccgatggc gttgtcgtat ctcaggtcgt gccgaactat cagaacgaca cgagcgacgt
    16021 cgtcgtgaag tacaaccagc tgatcaagga cgccggcaag ctgccgacgt tcacctcgct
    16081 cgagggctac atcgcggcgc gcatcttcat cgccggtctc gagcgccaca agggtccgtt
    16141 cacgccggac tcgctcgtcg acggcttcga gacgcttcct gacctgggcc ttggcctcgg
    16201 tgcatcgagc ggcttcaagc ccgacaacca ccagtactcg cagtcggtct ggggcacggt
    16261 gatccaggcg gacggaacgt tcaagaacct ctacttctgg agcaagggca ttccgatctc
    16321 gttctacgac taggtggtac cccctatgga agaagcaacc aaaagccatt ctgtaaccgt
    16381 cgggcgctac atgctgcata ggcagatcgc gcgcggcggt atggcaacga tccacatcgc
    16441 ccgtctcatg ggcgacgagg gcttctcgcg catcgtcgcg gcgaagcggc tgctccctga
    16501 attcgcggag gacaccgagt tcgtcgcgat gttcatggat gaggctcgca tcgcgtcgaa
    16561 ggtgcaccac ccgaacgtcg tgcccgtgct cgacctcgtc accacgaccg acgaagtgat
    16621 gctcgtgcag gagtacgtgc acggtgttcc gctgcacgtg ctgctgcgca ccgcgcatga
    16681 agcgggcaag aagattccgg tcaagatcgc ggtatcgatc gcggcgcagg tcctcgcggg
    16741 tctgcacgcg gcgcacacga cgctcgacga gatgggcgct ccgctcaaca tcgtccatcg
    16801 cgacgtgtcg ccgcagaacg tgatggtcgc ggtcgatggc tcggcgcgct tgctcgactt
    16861 cggcgtcgcg aaggcgacga tgggcgcgca catcacgcgc gagggcacgt tcaaaggcaa
    16921 gctcgcctac tccgcaccgg agcaggtgcg cggcaagccg tgccagcaga gcgatatcta
    16981 cgcgctctct gtcgtgctgt gggagctgct tgtcggccac cgcatgcatg gcagctcgca
    17041 aggcgaagca gagctcgtcg ccacgatcat gaacggcaac atcccgccgg tcaccgaagc
    17101 gctctcgaac gaagcggact ggggcgcgat cgacagcgac gagtggaaga tgctctcggc
    17161 gatcgagacc atcgtggcga agggcctcga tgtcgatctc gcgaagcgct acgacacggc
    17221 ggcggacatg gagacggacc tgcgcaaggc cgtgaccgtg gcgtcgtcga acgaggtctc
    17281 agcgtgggtg aagtcgctcg gcaaggactt catcgagagc cgcgacaagg tcatcgctgc
    17341 cgaagagcag agctggcgca ggaccgcgtc caccacgggt gttcgccgca cgccgctgcc
    17401 gggctcgctg ccgcgcccgt cgacgatcag cgaggtcagc caaccgcagc ttccgctcgc
    17461 gccgaaggaa gtgcgtcgta cgcgcatgat catcggcgtg ctcggcgcgc tcgtgatcct
    17521 gctcgcggtc ggcgtcgtcg tcgcgatgca gaccggcgac tcgaacaagc acgaggcgca
    17581 agctgcgccg gctccgacgc agcccacggc cgtcgagacc gagaagcaag ctccgccgcc
    17641 ggcagcgatg ccgacggaga agcaagagcc gaagcacgag acggcgaccg caccgccgca
    17701 gccgacgcag ccgactgaaa cggcggcgac ggaagccgct acgaccgaga ccaccgcgtc
    17761 gaccgagacg accgagacgg cgacgacgga gaagcccacg acgacgacga ctcgcaagtg
    17821 gggtcgtaac cgcgtcgtga agaaggcgga gcccgagaag aaagacccgc cgccggtcgt
    17881 aacccccgca gtcacgacag agaagccggc gaacgactgc agcccgccct actactacga
    17941 aggccagaag aaggtcttca aaccgaactg cctctaggag acacatgaag cgtcaactca
    18001 tcatcgcgtt cgcggtcctc agcttcacag cgtcgagcgc
//
LOCUS       DATLBZ010000133         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3047910, whole genome shotgun sequence.
ACCESSION   DATLBZ010000133 DATLBZ010000000
VERSION     DATLBZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3047910"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VMZ53_07160"
     CDS             <1..786
                     /locus_tag="VMZ53_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUS28268.1"
                     /translation="PYKQYKNAVTRAVGVYGSVEVDTFDFDILPGDRFLICSDGMYAY
                     IDEGELPKQLAEPEIKEVPKKLVDVANKGGGHDNITAVVIRVSEATGAEATPRSTDIA
                     QKLEVLKGMQMFRYLTYKELVQVAAIAETVDVKKDENIFTTGAPGDAMYVVVQGMVKL
                     VMGEQTVAQLSRGQHFGEIALIDRSTRSLTAIAAEKSQLVVVHRKAFVELIKREPASG
                     TKMLWSFVQVLGQRLRKTNADLSEALHGDKRGLSTENENKSQE"
     gene            complement(789..1952)
                     /locus_tag="VMZ53_07165"
     CDS             complement(789..1952)
                     /locus_tag="VMZ53_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HUS28269.1"
                     /translation="MRWMAVALVLAGCSFEHGTLGAISLDAPVDAGPAHCLDGWCLRK
                     PIQIAGAQVSGPQQDFVLLVRTAADPELAAASTTGEDFRFTTATGQPLPYERQRYVPA
                     TGELIAWVKLPMLPAADTTLYLYYGKAGVTDQQDVTATWGASYAGVWHLDETSTSTTA
                     ADASGNHNTGTALNVFSRLAAGGPTFGAAGAIGEAVTFDGADDELEMPKSTSLSATTG
                     LATFALWIKWTSLTAADYQRVLSSSNRFNSGVNEDGYEWAAQPAGDFFLYAWGGREDY
                     NLGPNPFTAGTWQYLVATLDFSTTSVKIYVDAQPMMFTTVNESAWSSKGDPGNWCWGA
                     NSNPTAAGPFGGGMDEIRVMHGAKDASWIATSYANQRPDSTMVTIGAQQILEP"
     gene            complement(1962..>2223)
                     /locus_tag="VMZ53_07170"
     CDS             complement(1962..>2223)
                     /locus_tag="VMZ53_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28270.1"
                     /translation="ADGSNLSNCWIEWGSTGWHAFTDIDQIIPLGATVRVRYHAHAGA
                     DACTFDGVATPLFDFPLEPNTYVAGLATTRGSPVRWVDVVSR"
BASE COUNT          402 a          743 c          701 g          377 t
ORIGIN      
        1 ccgtacaagc agtacaagaa cgcggtcacg cgcgcggtcg gtgtctacgg ctccgtcgag
       61 gtcgacacgt tcgacttcga catcctgccc ggcgatcgct tcctcatctg ctccgacggc
      121 atgtacgcgt acatcgacga aggcgagctc ccgaagcagc tcgcggagcc cgagatcaag
      181 gaagtcccga agaagctcgt cgacgttgcg aacaaaggcg gcggccacga caacatcacc
      241 gcggtcgtga tccgcgtcag cgaggctacg ggcgccgagg ccacgccgcg ctcgacggac
      301 atcgcgcaga agctcgaggt gctcaagggc atgcagatgt tccggtacct cacgtacaag
      361 gagctcgtgc aggtcgccgc gatcgccgag actgtcgacg tgaagaagga cgagaacatc
      421 ttcacgaccg gcgcgcccgg cgatgcgatg tacgtcgtcg tgcagggcat ggtgaagctc
      481 gtgatgggcg agcaaaccgt cgcgcagctc tcgcgcggcc aacacttcgg cgagatcgcg
      541 ctgatcgatc ggtcgactcg ctcgctcacc gccatcgccg ccgagaaatc acagctcgtc
      601 gtcgttcatc gcaaggcatt cgtcgagctg atcaagcgcg agcccgcgag cggcacgaag
      661 atgctgtggt cgttcgtgca ggtgctcggc cagcgcttgc gcaagacaaa tgcagatctc
      721 tccgaggcgt tgcacggcga caagcgcggg ctctcgaccg agaacgagaa caagtcgcag
      781 gaataggtct acggctcgag gatctgctgc gcgccgatcg tgaccatcgt cgagtccggc
      841 cgctggttcg catacgaggt cgcgatccag ctcgcatctt tcgcgccgtg catcacgcga
      901 atttcgtcca tgccgccgcc gaatggcccg gccgcagttg ggttcgaatt cgctccccaa
      961 caccagttgc ccggatcgcc cttcgaggac cacgccgact cgtttaccgt cgtgaacatc
     1021 atcggctgcg cgtcgacata gatcttcacg gatgtcgtcg agaaatcgag cgtcgcgacg
     1081 aggtattgcc aggtcccggc cgtgaacgga ttcggtccga ggttgtaatc ctcgcgcccg
     1141 ccccacgcat agagaaagaa gtcgccggcg ggctgcgctg cccactcgta gccgtcctcg
     1201 ttcaccccac tgttgaaccg attcgacgac gacaacacgc gctggtaatc cgcggccgtc
     1261 aagctcgtcc acttgatcca caaggcgaac gtcgccagcc ccgtcgtcgc ggacagcgag
     1321 gtgctcttcg gcatctcgag ctcgtcgtcc gcgccgtcga aggtaaccgc ctcgccgatc
     1381 gcgcctgccg cgccaaacgt cgggcctccc gctgcgagtc gcgagaacac gttgagcgcc
     1441 gtgccggtgt tgtgattgcc gcttgcgtcc gctgcggtcg tcgacgtcga cgtctcgtcg
     1501 aggtgccaca cccccgcgta gctcgctccc cacgtcgccg tgacgtcttg ctgatcggtg
     1561 acgccagctt tcccgtagta gaggtacagc gtcgtgtctg cggcgggcag catcggcaac
     1621 ttcacccatg cgatgagctc gccggtcgct ggtacgtagc gctggcgttc atacggaagc
     1681 ggctggcccg tcgccgtcgt gaagcgaaag tcctcgccgg tcgtcgacgc cgccgcgagc
     1741 tcgggatccg ctgccgtgcg gacgagcagc acgaagtcct gctgcggccc gctgacctgc
     1801 gcgcccgcga tctggatcgg tttgcgcagg caccatccat cgaggcaatg cgcgggccct
     1861 gcatcgacgg gcgcgtcgag ggatatcgcg ccgagcgtgc cgtgctcgaa cgagcaaccg
     1921 gcgagcacga gggctacggc catccagcgc atcgagtcat cttaccgcga cacgacatcg
     1981 acccaccgca ccggcgagcc tcgtgtcgtc gcaagccctg caacgtacgt gtttggctcg
     2041 agcgggaagt cgaacagcgg cgtcgctaca ccgtcgaacg tgcacgcgtc ggcaccggca
     2101 tgtgcgtgat agcggacgcg gaccgtcgcg ccgagcggaa tgatctggtc gatgtcggtg
     2161 aacgcgtgcc acccggttga cccccattcg atccagcagt tcgacaaatt agagccgtcg
     2221 gca
//
LOCUS       DATLBZ010000134         6411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7517945, whole genome shotgun sequence.
ACCESSION   DATLBZ010000134 DATLBZ010000000
VERSION     DATLBZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7517945"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VMZ53_07175"
     CDS             <1..538
                     /locus_tag="VMZ53_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HUS28271.1"
                     /translation="PATPESLIPGTPIIVPKLGRCEVAALPVDGKVEVRLGLMRATVP
                     IADVLIDSHRAARAAERERDRNHAEIDANGKPQGPTVVLIDGVPAGGRATARTIDSTI
                     DVRGQRVDEAVSMVDQFLDESLMASRDTAFIVHGHGTGALRQAIRTHLGAHKAIEKFR
                     PGEQSEGGDGVTVAFLKG"
     gene            complement(535..1785)
                     /locus_tag="VMZ53_07180"
     CDS             complement(535..1785)
                     /locus_tag="VMZ53_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS28272.1"
                     /translation="MRTLSLVALLCVSTTAWAQSKKYPPVEPDRDLEQESRSKTWDSA
                     LDPDRSPYQELVRDAKRLVEHGDANDIRIAIEKLDAAIKRMPKEADAYAVRGAIYLQQ
                     RQWVNCADDLAAAEAASKVEDITARTRSRIDLAVCQARAGRYAESEQTLVRAIASATG
                     FKGELMMRLGEVRIALGKLDEAIDALSASIDAADSLNDAARWLLAVAYDRARRPTDAQ
                     QFAVDAKRFDQSLSVISSPKMPFLGLGDTEYLYGIAYLFAKPTPEYALLYFKRFVAIA
                     GDSPWKRRAEEHVRDIGAMKFPSRDTLTLSSTTVSADDIRAALEKPMAQLRQCAAKTP
                     GTAYGVTITKSRTGPLSRETPMYRIPIEGVVANLMLDVDKVALKADIDAARDCMEKLA
                     TRISMPKPKDVNSYYRATFVVVAP"
     gene            complement(1782..2816)
                     /locus_tag="VMZ53_07185"
     CDS             complement(1782..2816)
                     /locus_tag="VMZ53_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28273.1"
                     /translation="MKRAALLVLLVASAAHANVWDDALRRGQPDAAQDKYDSEMKDGD
                     EHTLLANTRGSAPKEVRHQLQLAITSYKAAAAAKPTEGEPYFRLGRLVYSFYFECADS
                     NQIQAILRPPTCPDPRAPFDKNHAEEVIGYWNEFEKRAPLDPRLTVGAFGESEILFRR
                     AILHTKLATKAHLEAAAGDYERILARADDTSAAENVVGNLAETYMMVGRLEEAIETYR
                     QALRGASDTSTWYGFAVALDRDERGRQALEVIQSLGQEQREAFHRKVILGDTFFVPEG
                     EKYYYFALSDEAFGFDDEAIDGWKRFIDSGAHPQFQPRAKAHLDALLLKKKRKGLPVP
                     FDPPWRGILR"
     gene            complement(2813..3850)
                     /locus_tag="VMZ53_07190"
     CDS             complement(2813..3850)
                     /locus_tag="VMZ53_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS28274.1"
                     /translation="MRRLSIAVLAVLGLATPVRADADMIDEPADPEPAADRGNFWRNL
                     LTPHRDEVDLILSRARQALQTADMALYSDYDPTGTERIRFYKEIYGMLKYARRLEPDN
                     IEVLRLLGLTADEHGKTREAIEAFQSALDIAGDKAPSDIPGRLGIIYMRLGKLDDAIR
                     YLRMAQGPVMAGQPVSAHVLVHLSNALAARGQMTEAIDVLAANVPDTVQYFVNELLLV
                     SFALAVQYDRDEQRGAAFDVLDKMQNVLQGQLAMMGQQQLSGMRFSPPEDKHYYYALL
                     YESNGNYVEARAEWALYAAAGNLPFRRRALDHISALDAQARAPSADAANYGLAPPPAF
                     SPRIKHPRVTP"
     gene            4032..5045
                     /locus_tag="VMZ53_07195"
     CDS             4032..5045
                     /locus_tag="VMZ53_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28275.1"
                     /translation="MATASELYEARHYPGVVALVSDALDGEPDCVPLLLVRARAHIAL
                     RRDLDAQADLRDIIRLEPQCGVAYRLLGELAARRDENESAAIFFREALRLDPADREAS
                     QWLAIVDLSFRPAAVAQKLPAPAAAAGRFPPTRGPVQRAKTANDGNTRSVNAPARSPR
                     PGSQPRFASGTVSPDDERPTARYTSERERLLQKRQLTAPKTDEQPIPAGFMMSTDTPE
                     PLPPPPLRTATGSSPTLEPYRDPPRSAARGEGPTLKGAQPIRRPLSQPRAPSIPELPG
                     FGEYLVSTGILTRERLRAAQAYQRSMKVQLSTAIVTLGLATPQRIEWAAVAHQSQLPR
                     GNL"
     gene            5110..>6411
                     /locus_tag="VMZ53_07200"
     CDS             5110..>6411
                     /locus_tag="VMZ53_07200"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HUS28276.1"
                     /translation="MRSLAVFLIVLGACGFEHGAITEPDAFEIVPTVEFEDATSTADE
                     ASGIVQVAVVLSEDATDIVAVGYDVTGGNALRPDDFSLADGTVTFLPGQRRRTIDVTV
                     KPDTLEESDEDIVITLSGPSGATIGTTNHHTLTISADILPRVSFGNTATTSGNEATDE
                     TIQAILTIAPTQQVTVQLSVAGTATTADHALTDGQTITFAAGETMKTVPLGVVQDALD
                     EDDETVDLGLKNPSAKLLIAPTGTTHTRTITDDDSLPTVEYMATTSSTAESANATITV
                     TLTPVSGRIVTVPYSVTGGTANVADATVVGAPGTVTFMPGQTSRTISVTVMQDTLDEA
                     DETVAITLGAPTNATLGTNTVHTLTITDDDNPPTVSLGAANASAAEGNTGTSTVNLTF
                     TLSTASGLDVTIPYTVTGTATNSNTGDYTITASPVTIPAGMT"
BASE COUNT         1055 a         2206 c         2098 g         1052 t
ORIGIN      
        1 gcccgcgacg cccgagtcgc tcatccccgg cacgccgatc atcgtgccga agctcggccg
       61 ctgcgaggtt gccgcgctgc ccgtcgacgg caaggtcgag gttcgcctcg gcctgatgcg
      121 cgcgacggtg ccgatcgccg acgtgctcat cgactcgcat cgcgccgcac gcgcagccga
      181 acgagagcgc gaccgcaatc acgccgagat cgacgcgaac ggaaagccgc aaggtccgac
      241 cgtcgtcctc atcgatggcg tgcccgcagg tggccgcgcg accgcacgca cgatcgattc
      301 gacgatcgat gttcgcggtc agcgcgtcga cgaagcggtc tcgatggtcg atcagttcct
      361 cgacgagtcg ctgatggcga gccgcgacac cgcgttcatc gtgcatggtc acggcacggg
      421 cgcgctgcgc caagcgattc gcacgcacct cggcgcgcac aaggcgatcg aaaagttccg
      481 tccaggcgag cagagcgagg gtggcgacgg cgtgaccgtc gcgttcctca agggctaggg
      541 cgctaccacg acgaacgtcg cgcggtagta gctgttcacg tccttcggct tcggcatcga
      601 gatgcgtgta gcgagcttct ccatgcagtc gcgcgcggcg tcgatgtccg ctttgagcgc
      661 gaccttgtcg acgtcgagca tcaagttcgc gacgacgcct tcgatcggga tccgatacat
      721 cggcgtttct cgcgacagtg gaccggtgcg cgacttcgtg atcgtcacac cgtaggcggt
      781 gccgggcgtc ttcgcggcgc attgccgcag ctgcgccatc ggcttctcga gggccgcgcg
      841 gatgtcatcg gccgatacgg tcgtgctcga gagcgtgagc gtgtcgcgcg acggaaactt
      901 catggcgccg atgtcgcgca cgtgctcttc ggcgcgacgc ttccacggcg agtcgcccgc
      961 gatcgcgacg aaccgcttga agtagagcag ggcgtactcg ggggttggct tcgcgaagag
     1021 atacgcgatg ccatacaagt actcggtgtc gccaaggccg aggaacggca tcttcggcga
     1081 cgagatcaca gacagcgact ggtcaaagcg cttcgcgtcg accgcgaact gctgcgcatc
     1141 ggtcgggcgc cgggcgcgat cgtaggccac cgccagcagc cagcgcgcgg cgtcgttgag
     1201 cgagtcggcc gcatcgatcg aagcggacag cgcgtcgatc gcttcgtcga gcttgccgag
     1261 cgcgatgcgc acctcgccga ggcgcatcat gagctcgccc ttgaagcccg tcgccgatgc
     1321 gatcgcgcgc acgagcgtct gctcggactc ggcgtagcgg cccgcgcgcg cctggcagac
     1381 cgcgagatcg atccgcgagc gcgtgcgggc agtgatgtcc tcgaccttcg aggcagcctc
     1441 ggccgctgcg agatcgtcgg cgcagttcac ccattggcgc tgctgcagat agatggcacc
     1501 gcgcacggcg tacgcgtcgg cctccttcgg cattcgcttg atcgcggcgt cgagcttctc
     1561 gatcgcgatc cggatgtcgt tcgcgtcgcc gtgctcgacg agacgcttcg catcgcgcac
     1621 gagctcttga tacgggctgc gatccggatc gagcgccgag tcccacgtct tgctgcggct
     1681 ttcttgctcg agatctctat cgggctcgac cggcggatac ttcttcgact gcgcccatgc
     1741 cgtcgtcgag acgcacagca gcgcgacgag gctcagcgtc ctcaccggag gattccgcgc
     1801 cacggtggat cgaacggaac cggcaaccct ttgcgcttct tcttcaagag cagcgcgtcg
     1861 aggtgcgcct tcgcacgcgg ctggaactgc ggatgcgcgc ccgaatcgat gaagcgcttc
     1921 cagccatcga tcgcttcgtc gtcgaagccg aacgcttcgt cggacagcgc gaagtagtag
     1981 tacttctcgc cttcgggcac gaagaacgtg tcgccgagga tcaccttgcg gtgaaaggcc
     2041 tctcgctgct cctgcccgag cgactggatc acctcgagtg cctgtcgacc tcgctcgtcg
     2101 cgatcgagcg caaccgcgaa tccgtaccac gtcgacgtat ccgacgcgcc acgcagcgct
     2161 tggcgatacg tctcgatcgc ttcttcgagc cgaccgacca tcatgtacgt ctcggcgagg
     2221 ttgccgacga cgttttcggc ggcgctcgtg tcgtcggcac gcgcgaggat cctctcgtag
     2281 tctccggccg ccgcttcgag gtgcgccttc gtcgcgagct tcgtgtgaag gatcgcgcgc
     2341 cggaacagga tctcgctctc gccgaaagca ccgacggtga gtcgcggatc gagcggagcc
     2401 cgcttctcga actcgttcca atagccgatc acttcttcgg cgtggttctt gtcgaacggt
     2461 gcgcgcgggt ccggacacgt cggcggccgg aggatcgctt ggatctgatt gctgtccgcg
     2521 cactcgaagt agaacgagta gacgagccgg ccgaggcgga agtacggctc gccctccgtc
     2581 ggcttcgcag cggccgcggc tttgtaggac gtgatcgcga gctggagctg atgacgcact
     2641 tccttcggtg cactaccgcg cgtgttcgcg aggagcgtgt gctcgtcgcc gtccttcatc
     2701 tcgctgtcgt atttgtcttg cgcggcatcg ggctgcccgc ggcgaagcgc atcgtcccag
     2761 acgttcgcgt gcgcggcgct cgccacgagc agcacgagca gcgcggcgcg cttcatggcg
     2821 tcaccctcgg atgcttgatg cgcggcgaga acgcgggcgg cggggcgagg ccatagttcg
     2881 ccgcgtccgc agacggtgca cgcgcttggg catcgagcgc ggagatgtga tcgagggcgc
     2941 ggcgacggaa cggcagattg ccggcagcgg cgtacagcgc ccactccgcg cgggcctcga
     3001 cgtagttgcc gttcgattcg tagagcagcg cgtagtagta gtgcttgtcc tcgggcggcg
     3061 agaaccgcat cccggagagc tgctgctggc ccatcatcgc gagctggccc tgcagcacgt
     3121 tctgcatctt gtcgagcacg tcgaacgcag cgccccgctg ctcgtcgcga tcgtattgca
     3181 cggcgagcgc gaagctcacg agcaacagct cgttgacgaa gtactgcacc gtgtccggca
     3241 cattcgcggc gagcacgtcg attgcttcgg tcatctggcc gcgagcggcg agcgcgttcg
     3301 agagatgcac gagcacgtgc gcgctgaccg gctgcccggc catcaccggt ccctgcgcca
     3361 tgcgcagata gcggatcgca tcgtcgagct tgccgaggcg catgtagatg attccgaggc
     3421 ggcccggaat gtcggacggc gccttgtcgc ctgcgatatc gagcgccgat tgaaacgcct
     3481 cgatcgcttc gcgcgtcttg ccgtgctcgt cggccgtgag accgagcagg cgtagcacct
     3541 cgatgttgtc gggctcgagc ctccgcgcgt acttcagcat cccgtagatc tctttgtaga
     3601 agcggatgcg ttcggtaccg gtcgggtcgt agtcgctgta gagggccatg tccgcggtct
     3661 ggagcgcctg acgcgcgcgc gacagaatga ggtcgacctc gtcgcgatgc ggcgtgagga
     3721 gattccgcca gaagttgccg cgatcggcgg cgggctctgg atcggccggc tcgtcgatca
     3781 tatcggcgtc ggcgcgcact ggggtcgcga gcccgagtac cgctaacacc gcgatcgaaa
     3841 gccgccgcac tcgcgaagta tgccggtcta aacgcgggct tgccagctga catccctggc
     3901 ctctggggac aggcacgtgg ggtcccgacc ctccggcggg atcgcacgga ttcgccgtcg
     3961 ttccgcaggg ttgagcagtg ccgagccgtg gcccacccct tgctcttctc ctgaccgtgg
     4021 atcacgcagt catggctacg gcgagcgagc tgtacgaggc tcgccattat ccaggtgtcg
     4081 tcgcgctcgt cagcgacgca ctggatggag aacccgactg cgtgccgctg ctgctcgttc
     4141 gcgcgcgcgc tcatatcgcc ctgcgccgcg acctcgatgc acaggccgac cttcgcgaca
     4201 tcatccggct cgagccgcag tgcggcgtcg cgtatcggtt gctcggcgag ctcgccgcgc
     4261 gccgtgacga gaacgagtcg gccgcgatct tcttccgcga ggcgctgcgc ctcgatcccg
     4321 ccgaccgcga agccagccag tggctcgcga tcgtcgacct ctcgtttcga ccagccgcgg
     4381 tggcacaaaa actcccggca cccgctgccg cggctggtcg ttttccccct acgcgtgggc
     4441 ccgtgcagcg tgcaaagacc gcgaacgacg gcaacactcg ctcggtcaac gcacctgcac
     4501 ggtcgcctcg ccccggcagc cagcctcggt tcgccagcgg caccgtctcg cccgacgacg
     4561 agcgcccgac cgcgcgctac acgtccgagc gcgagcgcct gctgcagaag cgccagctca
     4621 ccgcgccgaa gaccgacgag cagccgattc cggccggctt catgatgagc acggacacgc
     4681 ccgagccgct gccgccgcca ccgctgcgca cggcaaccgg aagctcgccc acgctcgagc
     4741 cgtatcgcga tcccccgcgg tcggcagcac gcggcgaagg cccgacgctc aaaggcgcgc
     4801 agccgattcg ccgcccactc tcgcagccgc gcgcgccatc gatccccgag cttcccggct
     4861 tcggcgagta cctcgtctcg acgggaatcc tcacgcgcga acggctccgg gccgcgcagg
     4921 cgtaccagcg ctcgatgaag gtccagctct cgacggcaat cgtgacgctg ggtctcgcga
     4981 cgccgcaacg catcgaatgg gctgcggtag cgcaccagtc gcagcttcca cgcgggaacc
     5041 tctgagcgcc cgtcgagtgt gatctggtgg gcttgtgccc gccgcccgac gctgagatca
     5101 tgtcggcgcg tgcgtagcct cgccgtcttc ctcatcgtcc ttggagcttg cggcttcgag
     5161 cacggcgcga tcaccgagcc cgatgcgttc gagatcgtgc cgaccgtcga gttcgaagac
     5221 gcgacgagca cggccgatga agcatcgggc atcgttcagg ttgccgtcgt gctctcggag
     5281 gacgccaccg acatcgtcgc cgtcggctac gacgtcacgg gcggcaacgc gctgcggccc
     5341 gacgacttct cgctcgccga cggcaccgtc acgttcctgc ccggccagcg gcgccgcacg
     5401 atcgacgtga cggtcaagcc ggacacgctc gaggagtccg acgaggacat cgtcatcacg
     5461 ctgtcgggtc cgtccggcgc aacgatcggc acgacgaatc accacacgct gacgatcagc
     5521 gcggacatct tgccgcgcgt cagcttcggc aacaccgcga cgacgagcgg caacgaagcg
     5581 accgacgaga cgatccaagc gatcctgacg atcgcgccga cgcagcaggt caccgtgcag
     5641 ctctcggttg ccggtacggc gacgaccgcg gatcacgcgc tcaccgacgg ccaaacgatc
     5701 acgtttgctg cgggcgagac gatgaagacc gtgccgctcg gcgtcgtgca agacgcgctc
     5761 gacgaagatg acgagaccgt cgacctcggg ctgaagaacc cgtcggcgaa gctgctgatc
     5821 gcgccgaccg ggactacgca cacgcgcacg attaccgacg acgattcact gccgacggtc
     5881 gagtacatgg caacgacgtc gtcgacggcc gagtcggcaa acgcgaccat cacggtcacg
     5941 ctcacgccgg tctcgggtcg catcgtcacg gtcccgtact cggtcactgg cggcacggcg
     6001 aacgtcgcgg atgcaaccgt cgtaggcgcg cctggaacgg tgacgttcat gcccggccag
     6061 acctcgcgca cgatcagcgt gacggtcatg caggacaccc tcgacgaagc agacgagacg
     6121 gtcgcgatca cgctcggcgc tccgaccaac gccacgctcg gcacgaacac cgtgcacacc
     6181 ctgacgatca ccgacgacga caacccgccg accgtgagcc tcggtgccgc gaacgccagc
     6241 gccgccgagg gcaatacggg aacatcgacc gtgaacctga cgttcacgct gtcgaccgcg
     6301 agcggcctcg acgtcacgat cccgtacacc gtgacgggca ccgcaacgaa ttcgaatacc
     6361 ggcgactaca cgatcacggc ctcgcccgtg acgattccgg caggcatgac g
//
LOCUS       DATLBZ010000135        20310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4599108, whole genome shotgun sequence.
ACCESSION   DATLBZ010000135 DATLBZ010000000
VERSION     DATLBZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20310
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4599108"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VMZ53_07205"
     CDS             complement(<1..450)
                     /locus_tag="VMZ53_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28277.1"
                     /translation="MRIVSTSNLPIDLNGQVRAQIADAIVEVPEQWVDVARCDLSTAD
                     ALVCLLLDKIDASVIARAPRLRVIANCAVGYDNVDVAAATRAGIAVTNTPDVLTDATA
                     EFTVALIFAAARRLPEGEGLVRSGQWPGWRLDQLLGQPIAGRTLGVVG"
     gene            500..1474
                     /locus_tag="VMZ53_07210"
     CDS             500..1474
                     /locus_tag="VMZ53_07210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUS28278.1"
                     /translation="MRWASLAIVALLALPASSTDARRGPKVVAIAKLPAPPSTPEASA
                     PEATDDEAAKAAKAALAAATNDPIFAGADRVVGRDQPGYVAFTFDDGPTPATTPVVLD
                     ALQTYDIPATFFIVTRHLNHPRDREQARQLLAKELELGFTVGNHTAHHARLEHTTDEK
                     LLVREVDEAVMTLTAHAKRPIGLFRAPYGKLSTFAKDHLHEMGVTDVFWSIDSRDWET
                     AEEDAAELRRSILEMIVADNGGVVLMHDTRKVTAKVIRDVFDDLEHENCRRLAKHETP
                     IWPVSLHYFLRDAGVPRAIPDDVQKRTAAYQQALPDRCALRARKTGRK"
     gene            1650..4430
                     /locus_tag="VMZ53_07215"
     CDS             1650..4430
                     /locus_tag="VMZ53_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS28279.1"
                     /translation="MPLTWDRHTPAPGSISVHEQGTEPTPTFEPSDLGSGDGFGEGEG
                     ERTVITAATPYDLRPKFTGRAQGIQQLQELLDTAWKQNQIAFAVVIGEPGMGKSRMVG
                     EVIARAKTRHPNVQVLQGIADENAHAYGPIARALTVRFGLQPGEDPAESRDKIQAGVA
                     EVVQASRVTEIAHLIAHLLRVPFDDSPVITPLLDSPQRLEARLFMALKRLLAAEAEKR
                     PVIVVVENLELCGTDTINFLQYLAAGMRENRVAVLCTATADVWKRHAAFGEGEVAPAK
                     VELGALSPQESEQLLRELCKQLSEVPAKLVTHVKNLGGSPRAIHELVRLLLESEVIAR
                     EGYLWRLDPAKLAAMTLPKSYEELVAARLRVMETTERRVLEMAAVVGETSWLDAIIAI
                     ERLSSPVKDPDGPTLSQIASSGDHSRLSVVAAIGKLVEREWIVEVPQSSVSGEREVRF
                     AYPNLWSIVYKSIDEARRRSYHAVVARWLELHPEGRTPSVQEEVGRHLALAGHPRDAA
                     LRYRRAAEAARSQFANERAIRLYDRALACIGEHDPSMRIFLWHDLGSIYELIGDFEAA
                     LGAFERMLRLSWVSASKTKAAVAFNKMGRVWRRKGDLKLALEYLERGLELFRGASDAR
                     GIAGSLDDIGKTLQMLGRYDEAHAKITEALARRGKHGDKRAIATSLSRLGDVQQDRGQ
                     YESAYNCHKDAYELRKAAGDRWGCVVSQNNLAALAFELGDAAEARATWLAALPESEAI
                     GALPLEALILTNLGEVALVEGKLDEARTRLENALEIVEDIDDRGLESECCRHLATLEK
                     SQGHPTAAREHAERALEVAKKAGLREKEAQAYMTLGDVLSASLYDAEDAQSATTDAAV
                     AYGKAIDVLKQIGNEAALGKALFAFGRYKAETGAIAAGKDMLRDALTLFAKLGLKRPH
                     DDANKLLSTLSA"
     gene            4471..5502
                     /locus_tag="VMZ53_07220"
     CDS             4471..5502
                     /locus_tag="VMZ53_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HUS28280.1"
                     /translation="MIWFWLGFFGLVALLLFLDLGVLHRKDQEQSLKTAAMWTVAWVI
                     LGLAFSAVVYLIYENHWLDAKLKHTPVGHADGGEAAVTYISAYLLEQALSIDNIFVIA
                     LTFAQFKVSPQFQHRVLFWGILGAVVFRVAMLGGGVWLASKFDWIFYIFGGFLAYKGA
                     ELLKGFVAKMRAKGEEEEEESELPWLVRKLRRVIRIEENHHGHFAVEINGKKVLTILA
                     LCLITVELQDIVFALDSIPAVLSVSNETFIIVTSNIFAILGLRSLYFVLAGMMAKFEY
                     LELALGVLLIGIGVKMVLHNHVHISHIISLLAIAGILAAGVIASVIRMRNAANQQPPI
                     EPGPASPSP"
     gene            complement(5468..6430)
                     /locus_tag="VMZ53_07225"
     CDS             complement(5468..6430)
                     /locus_tag="VMZ53_07225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS28281.1"
                     /translation="MDVIDLAACADDFDREVALTPEIDRFCSASAWILSASAALMPPR
                     ASFTFRGKSGYLAMMRGVHPAGFPYLEPVELAWGLAAPFIGQDAAALIAEVVPLLAAR
                     RDWQLAIVSGHTVSGPQRRALDATLPARWERRQGQPTIRHVASLDGGIDGFLSRRSRE
                     LRKSLRKTERAARERGMTFDSVRATQREALALYDRIQAVEARSWKSHEGVGIHAGPMR
                     AFYGQMLPRLCELGQQRTIFAQLDGQDIGYILGAVMGDEYRGLQFSYDDGYAELGIGG
                     LLQLQQVRELCSEGIARYDLGTEMDYKRRWAEEIMETEMLVLVR"
     gene            6476..7543
                     /locus_tag="VMZ53_07230"
     CDS             6476..7543
                     /locus_tag="VMZ53_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28282.1"
                     /translation="MRLVSLVVATTVAATGCATTSYKIPTSELNRIASQPPETRGQHV
                     RVMQELDDTDAGPPQPVTAETQIIFFPQINVYGPSERRRYYQTNSTWGCGGCGGGGVS
                     GGGGGHKSGGGKGFNFNGGAGDGKGAAVAVLVAAAVALVAVAAVEGSRFDGFANLHPM
                     HPVYMIGKNGEHIVLPLAWIDPETAKWTDKAVIRSTEGPWQPLERAPLDRQGWTYAMY
                     GGVGTFESADGSKDNGTATTIQLGYFPTHEVGIVGSVFFGWRQNAVDQTLFESRYTLE
                     LQGYPIHTGPMQFGGYIGGGAAYRFEDGIQGGNSGSSALLGGAMMQLDINTRLALTAR
                     FGLTRAHEERMTDAMVGLSVY"
     gene            complement(7544..7828)
                     /locus_tag="VMZ53_07235"
     CDS             complement(7544..7828)
                     /locus_tag="VMZ53_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HUS28283.1"
                     /translation="MATDIPEPTRIEIEAAAFRRLVKHFQERTDVQNIDVMKLAGFCR
                     NCLSKWYRAAAEERGVALSDPEARQAIYGMPYEEWKAKYQTDEKKPHEGH"
     gene            7861..9018
                     /locus_tag="VMZ53_07240"
     CDS             7861..9018
                     /locus_tag="VMZ53_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HUS28284.1"
                     /translation="MTTVEHEGTTYYILGTAHVSQRSVDEVQAAIKELQPDVVCVELD
                     QARHDALMKDSAFRDLDVFKVVREGKTLYLLAHLALSSYQRKIGASLGVKPGTELLAA
                     VKTANEAGIPVELIDRDINITLKRTWKNLGLWKRSMLISSLLVGFEDDEDGAPVTSET
                     VENLKEPKALSEMLTELGKAVPEVKGPLVDERDQFLVSKMLEAGRGKKKVLAVVGAAH
                     VPGMLENINVAIDREKLNKVPPPSLIWQILKWGIPLAFVVGMIYFWQTSTTTTLAQMM
                     LAWILPTSIGAAGLTLIAGGSLLTVLAAFIVAPIAAIHPLLGTGMVTGIVEAWRRRPS
                     VQDCERLPDDVQSFKGFWRNPVTRILLVAVASGLGTAVGFWVGVGWVASLL"
     gene            9065..10540
                     /locus_tag="VMZ53_07245"
     CDS             9065..10540
                     /locus_tag="VMZ53_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HUS28285.1"
                     /translation="MINRRQAIKRIGGLAGLAAMPKMLSGCGDDGGSDTPGIKNYVYM
                     MLENRTYDHVFGARKLQGLGGDGLTTGMALPDMNGTMIPIFEPSANEMCVADPPHGWD
                     PSHAQFNGGAMDGFIRQYQMAEPQAPAKQVMQYMTRTQMPVSWALADEYTTCDRWFAS
                     VMGPTLPNRAYWHAATSFGLKVNNEVLTQFSAPPVPTIYNKLHDKNVDWVYYSGPLAV
                     ASLLGNPGPYMLDLGPTDGTGHIRRFSAYADLPDDPNGQFFKDAKAGKLAPVTYIDPF
                     FGENDDHPPLHPIMAQALIAAVYNALAQSPQWKHTMLVITYDEHGGFYDHVAPPKTTD
                     TTEADFGVSGFDQMGFRVPAMVIGPYAKKNYVSSTVYDHTSALKHLTNVFGLESLNAR
                     VDAANDLMDCIDMDRLMAGDPADPIDLPTIDLSQYAQYMTDPDCKGMAFRPLDPVSEW
                     ADLNPGIMLDYRSPNERNAYHRGIMKALDEQQRNRALVKSR"
     gene            10575..10823
                     /locus_tag="VMZ53_07250"
     CDS             10575..10823
                     /locus_tag="VMZ53_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28286.1"
                     /translation="MKKLSLIIAAGSLALATPVFACPGHDSNETKTAEKAPAKEEPKK
                     EEPKKEQAPTTTAKAKEAPKKEAPAKDAPAKPTKVSQK"
     gene            10932..12386
                     /locus_tag="VMZ53_07255"
     CDS             10932..12386
                     /locus_tag="VMZ53_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28287.1"
                     /translation="MCAVARAQQMPCEPCVRGDDTLRQWGPAGEELRTKVQGVTSLGI
                     GTLEMNERDAQQLDIYIGALREPLRARIQQLSDTQLSEVAAALCNAPDGGCVTNMVIA
                     MQCIAERCTIRHEQHNDERMLPVDPPGLPTCDPVADKTKSSKAAIGFEWATGWQDDAS
                     PTDHRAWSLGFEARTRLNKRAGLVGRVDRSTGRDKANDADRDGRDDFGTGPVTRVLVL
                     AGPSYAFAVAHDKDIVRYLQLDLLGGYQWTLSKEDEDGFAAGADLSYTLAVARVGMRF
                     TQGFGDAEDARAVLAHVGFVVGGAPPVDYGSGCDRPSRTPTKLALALDIPLFGYGMSD
                     QLDYIVPGFGVEALYHFTPSFDGFVHGDIIDAPNGGRDRAVYQTLLAGARLDLASRDG
                     EHGTKTGVFSTLSAGYTWAATTDSSTAGSGPVAEAAIAWGGQGDDGGAYLKLHARFGL
                     VPDNYDMRALFLSGGVELRLDRRKWRDRMGGKND"
     gene            complement(12383..13243)
                     /locus_tag="VMZ53_07260"
     CDS             complement(12383..13243)
                     /locus_tag="VMZ53_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUS28288.1"
                     /translation="MGPTASELRTIPLFQGFGDAELNTVAGLFTQVEASDKPLFELTE
                     PATTLYLLTKGEVVLDRPGDDVFRLHPPALIGELGALTGLSRSTRANVTPGSVVWALP
                     AKKLQAYLADNQELGVRFLVNLLQTVADKVHRDQRRMADMRTNLITTQKELKRLRELV
                     LDAPETPISAPVHDTLDKLITHNRRVNYRVEPPPALAATCRFDSATSPIADISRTHLT
                     VSLATEPPAIGTWVSGVAQLAGTEIPISGRVHRVQGKRMTLELDLMIDEFAAALEGYL
                     TRAQLLDILC"
     gene            13275..14156
                     /locus_tag="VMZ53_07265"
     CDS             13275..14156
                     /locus_tag="VMZ53_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28289.1"
                     /translation="MRVWTLVLLVACGKDAGPAKKPEAARKDAGAAAAVAADAAGPSV
                     EADSFADLGIALETIIPPDARVVGFGELHNRTDRAQVKSSLAHFTQEGLPAISDKMSD
                     LIVETWVVDPKCGQQAKTATAKVEITVRRPQETKSEIALLAEAARAAKIQPHAMKIKC
                     DEYDKIAPQGKEVAPDVLLDLTTRELGRISEEAVKHRDKEPEHRPWIALYGGALHNDR
                     FPAKGVESWSYAAKVDGLTANHYVEVDLIVPELAELDPGSQSAPWFPLVQSKDDKVKV
                     FKRGERSYVIVLPRTPG"
     gene            14153..14941
                     /locus_tag="VMZ53_07270"
     CDS             14153..14941
                     /locus_tag="VMZ53_07270"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HUS28290.1"
                     /translation="MIGAIAARIAEAWLLCAILQCVLWFVAEKTKNAGIVDVGWAQSF
                     TAVILVFAVQPLTPRSNWLPIAIVVIAWSTRLTCYLIARGAATGREEGRYVDLRRRWA
                     LHASKRFFVFFQAQAALAALLATAFVVPFVSPAWDGGIVRAFGALVAAAGVAGEAAAD
                     AQLRAFKKDPANVGKVCDTGLWSYSRHPNYFFEWLVWLGFALYGLAFAPAGLVALLGA
                     AIIFGSIWGVTGIPPTEAQALRSKGEAYRDYQRRVSKFIPLPRK"
     gene            14958..15689
                     /locus_tag="VMZ53_07275"
     CDS             14958..15689
                     /locus_tag="VMZ53_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUS28291.1"
                     /translation="MHGPGSRNKLSPSHARHYPGDGLLDTLGRAICAVDCLPRKELHE
                     AWEMATRIRAWLPSTPTGRVVDLCAGYGLLAQVLLLLDTDMRSTALAVDIRLPKNHVI
                     VHDAVVTTFPSLAGRVTFQRARLEDVDVGVGDVIVSAHACGALSDAVLSRASAVCARV
                     ALLPCCHLTRWRPDLVDRADPAARIDEERAMWLVERGYDVIVDSIPANVSAKNRLLLG
                     RPRAHPHRAEPAIERGDDSLRRSRS"
     gene            15846..16616
                     /locus_tag="VMZ53_07280"
     CDS             15846..16616
                     /locus_tag="VMZ53_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28292.1"
                     /translation="MFKRTVTTIVLGALLGACRPKSPHYETGPCEESIRARAAGKYVN
                     QTHAEAEQRNYFYFASCNDLCDDDHERECLELGLLEDDFGVNPAEPILSPLCAKGNKA
                     ACDWVAANPEKVASSQNYTKVVLARRAAGSDERPTSQSDLASFAAQLKRAAIGFSTLR
                     DETLSLGGGYTTFKINVTLGRKYLVVCAVEDPSVITARMESGGYTLELEPGHNQLPGV
                     FNVQLSFEAGDAGASAKVFVRSASGPQRSVRCFLLEKD"
     gene            complement(16663..17718)
                     /locus_tag="VMZ53_07285"
     CDS             complement(16663..17718)
                     /locus_tag="VMZ53_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HUS28293.1"
                     /translation="MRIAHALVMTCACGRIAFDPLSDAAVPTLRSQLTLAAPAEPLTD
                     FPLLVVLDDTRAARDRMQPDASDLRFFDSAGTLLPHEIEQVGSAGGAPLVAWVRVPII
                     DGPTTLTIDYGHGTPPPASTQSVWAAPYAAVYHLGDRNDSTQHALAGTWSGGPETVSD
                     GQIAKAHTFRTTNSDSIVVADAPAFAFGSGITVSCWMNPSSFVAASGYNAAVTQEAMG
                     SSEAFWIGIEDSTRIAYAELGTASTFNQGGGGGTLTAGTWLHAAMTYDGTTERFYING
                     MQRNVTSVIGGALFASGNPVLLGADANSGAGPDADFFDGMIDEARIETVPRSASWIAY
                     DVASMRDEVITYGTVGP"
     gene            17743..18219
                     /locus_tag="VMZ53_07290"
     CDS             17743..18219
                     /locus_tag="VMZ53_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28294.1"
                     /translation="MRIMALVVLLVAACGSKKSDNAAPKGAKVIEWDATKSSRATYKT
                     ATFDNRVTRDDIISVKACADEIKLKLHVDTATAKYTEDGKPVEIVALASLTATVEDGK
                     GFELTNGACDGPNYQLTAPGEPPGYTILDCHFKAKKPNNDCAPMFQLKGDGTLLTN"
     gene            complement(18216..18908)
                     /locus_tag="VMZ53_07295"
     CDS             complement(18216..18908)
                     /locus_tag="VMZ53_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28295.1"
                     /translation="MVRFTGLVFALALVAGCAAKSSEKATTDRGAGATGGSPTPSPAD
                     QTPVTTESPQNEAPNTTGAIPPGGPSQGGKPLDPSANADPNSPKQQARTNGMLGATDR
                     FDDSPLKDDKTPTTTKGKTGGKTAETKPALVGGSEAGGEAALAFDTPDEAVKTALDAQ
                     RAALTTCHKAGTGTLEIAITVDANGKVTSAKIAASSTLKDTTTRNCVLAVIKKLQLGK
                     GAKVTAPIRITF"
     gene            complement(18940..19455)
                     /locus_tag="VMZ53_07300"
     CDS             complement(18940..19455)
                     /locus_tag="VMZ53_07300"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HUS28296.1"
                     /translation="MRDTLVLTLIGNDRTGLVQSVAEKLSSLGANWEEARMARLAGQF
                     AGILLVTIEAGKTDELVASLRTLDAAGLQVTARRTAAPEPATDHSVLKLTVTANDRPG
                     IVRDVSRVLAERGINVEDLETEVTSAAMSGGPLFNYRALIRAPGGVALDDLRARLEAL
                     GSELMVDLSST"
     gene            19518..20012
                     /locus_tag="VMZ53_07305"
     CDS             19518..20012
                     /locus_tag="VMZ53_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HUS28297.1"
                     /translation="MIRSPERGSAMLVTLIIITALMAGAATLTQMQLTSTRSSSLVRD
                     KMASLHCAEAGLAVARAAVTANVTSWNSALAMTTEPSWLQSLPHDIDGDGVRDFTLRL
                     RDNADETVDNPNVDNDLAVFVVSSCVKFTDAPAEVMELVRFNGGGSCYQAQLGGCGGN
                     SNAN"
     gene            20002..>20310
                     /locus_tag="VMZ53_07310"
     CDS             20002..>20310
                     /locus_tag="VMZ53_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28298.1"
                     /translation="MRTELIVVTIAILAAPSIVDASCVPGFDFAIFAKDTLQIQGNAG
                     TDAWNSATGTYAATKTCANADIGTNSSTSGAAHIQSNSTMICGDAYSGAGSNPTAVYT
                     G"
BASE COUNT         3581 a         6656 c         6603 g         3470 t
ORIGIN      
        1 cccgacgacg ccgagcgtgc ggccggcgat cggctgaccg agcagctgat cgaggcgcca
       61 acccggccac tgcccggagc gcacgagccc ctcgccttcc ggcaatcgac gcgcagcggc
      121 aaagatcagc gcgacggtga attccgcagt cgcatcggtg agcacatcgg gtgtgttcgt
      181 caccgcgatg ccggcccgcg tcgcagcggc cacatcgaca ttgtcgtaac caacagcgca
      241 gtttgcgatg acgcgcagcc gtggcgcgcg cgcgatcaca ctcgcgtcga tcttgtcgag
      301 gagcaggcag acgagcgcat ctgccgtcga caaatcacaa cgcgcgacgt caacccattg
      361 ttccggcacc tcgacgatcg catctgcaat ttgcgctctc acctgcccgt tcaagtcgat
      421 gggcaggttc gacgtcgaga cgatgcgcac cgcgagagga tactggatgc ggcgaacccg
      481 cgtgttacgc ttatttcgaa tgcggtgggc ctcgctggcg atcgtcgcgt tgctcgcgct
      541 gccggcgtcg agcaccgacg cgcggcgtgg gccgaaggtc gtcgcgatcg cgaagctgcc
      601 agcgcccccg tcgacgcccg aagcgtctgc gccggaagcc actgacgacg aagctgcgaa
      661 ggccgcgaag gcggcgctcg cggcggcgac gaacgatccg atcttcgccg gtgccgatcg
      721 cgtggtcggt cgcgatcagc caggctacgt cgcgttcacg ttcgacgatg gcccgacgcc
      781 ggcgacgacg cctgtcgtcc tcgatgcgct gcagacgtat gacattccgg cgacgttctt
      841 catcgtcact cgtcacctca atcatccgcg agaccgcgag caagcgcgcc agctcctcgc
      901 caaggagctc gagctcggct tcaccgtcgg caatcacacc gcgcatcacg cgcgcctaga
      961 gcacaccacc gacgagaagc tgctcgtccg cgaggtcgat gaagccgtca tgacgctcac
     1021 cgctcacgcg aagcgaccga tcggcctgtt tcgcgcgccg tacggcaagc tcagcacgtt
     1081 cgccaaggat catctgcacg agatgggcgt gaccgatgtg ttttggtcga tcgactcgcg
     1141 cgattgggag acggccgaag aagatgccgc ggagctgcgg cgctcgatcc tcgagatgat
     1201 cgttgcggac aacggcggcg tcgttctgat gcacgacacg cgcaaggtca cggcgaaggt
     1261 cattcgcgac gtgttcgatg acctcgagca cgagaactgc cgccgcctcg cgaagcacga
     1321 gacccccatc tggcccgtct cacttcatta cttcctccgc gacgcgggcg tgccacgcgc
     1381 gatccccgat gacgtccaaa agcgaaccgc cgcctatcag caggcgctgc cggatcgctg
     1441 cgcgctacga gcccgaaaga ccggacgaaa gtaagcctcg ttcgtccgcg tcgggcgaca
     1501 ttttcccgtg gcattgaagc cacttctccg tggttgacaa ctacatcgaa ccacattagg
     1561 gtccgcgcgc tttacgggcg gaataggcac gtggaaatct catggaatca tcgaacgttg
     1621 tccgcgcagt cggcgctgac tggagcccca tgccgttgac ctgggatcga catactcccg
     1681 ctccgggttc gatcagcgtc cacgagcagg gcaccgagcc aacgccgacc ttcgagccga
     1741 gtgacctcgg ttcgggcgac ggattcggcg aaggtgaagg cgaacgcacg gtcatcacgg
     1801 ccgccacgcc ctacgacctc cgcccgaagt tcaccggtcg cgcgcagggc attcagcagc
     1861 tgcaagagct gctcgacacc gcatggaagc agaatcagat cgcattcgcc gtggtgatcg
     1921 gcgagcccgg catgggcaag agccgcatgg tcggcgaagt catcgcgcgt gcgaagacgc
     1981 ggcatccgaa cgtccaagtt ctgcagggca tcgcggatga gaacgcgcat gcgtatggcc
     2041 cgatcgctcg cgccctgacc gtgcgcttcg gcctccagcc cggcgaggat ccggcagaga
     2101 gccgcgacaa gattcaagct ggcgtcgccg aggtcgtgca agcctcgcgc gtcaccgaga
     2161 tcgcgcacct catcgcgcat ctgttgcgcg ttccgttcga tgactcgccc gtcatcacgc
     2221 cgctgctcga ttcgccgcag cgactcgaag cgcgtctgtt catggcgctc aagcgcttgc
     2281 tcgccgccga ggcggagaag cgccctgtca tcgtcgtcgt cgagaacctc gagctctgcg
     2341 gcaccgacac gatcaacttc ctccagtacc tcgccgctgg catgcgcgag aaccgcgtcg
     2401 ccgttctgtg cacggcgacc gccgatgtgt ggaagcgcca cgccgcgttc ggcgaaggcg
     2461 aagtcgcgcc tgcgaaggtc gagctcggag cgctgtcgcc gcaggagagc gagcagctgc
     2521 tccgcgagct gtgcaagcag ctctcggaag ttccggcgaa gctcgtcacg cacgtgaaga
     2581 atctcggcgg ctcgccgcgc gcgatccacg agctcgtgcg cctgctcctc gagagcgaag
     2641 tgatcgcccg cgagggttat ctctggcggc tcgaccccgc gaagctcgcc gcgatgacgc
     2701 tgccgaagtc gtacgaggag ctcgtcgccg cacgccttcg cgtgatggag acgacggagc
     2761 gccgcgtgct cgagatggcc gcggtcgtcg gcgagacatc gtggctcgac gcgatcatcg
     2821 caatcgagcg tttgagctct cccgtcaagg atccggacgg tccgacgctc tcgcagatcg
     2881 cctcatcggg tgatcactcg cgcctgtcgg tcgtcgctgc gatcggcaag ctcgtcgagc
     2941 gcgaatggat cgtcgaggtc ccgcagtcga gtgtgtcggg tgagcgagag gttcgcttcg
     3001 cgtatccgaa tctgtggtcg atcgtctaca agtcgatcga cgaagcacgc cgccgcagct
     3061 atcacgcggt cgtcgcgcgc tggctcgagc tgcatcccga gggccgcacg ccgagcgtcc
     3121 aagaagaagt cggccgtcac ctcgcgctcg caggtcatcc gcgcgatgca gcgctgcgct
     3181 accggcgcgc cgccgaagcc gctcgctcgc agttcgcgaa cgaacgcgcg attcgcctgt
     3241 acgaccgcgc cctcgcctgc atcggcgagc acgatccgtc gatgcgcatc ttcctgtggc
     3301 acgacctcgg ctcgatctac gagctgatcg gcgacttcga agccgccctc ggcgcgttcg
     3361 agcgcatgct gcgcctgtcg tgggtgtcgg cgagcaagac gaaggccgcc gtcgcgttca
     3421 acaagatggg ccgcgtgtgg cgtcgcaagg gcgacctcaa gctcgcgctc gaatacttgg
     3481 agcgtggcct cgagctgttc cgcggcgcgt cggacgcgcg cggcatcgcc gggtcgctcg
     3541 acgatatcgg caagacgctg cagatgctcg gccgctatga cgaggcgcac gccaagatca
     3601 ccgaggcgct cgcgcgtcgc ggcaagcatg gtgacaagcg cgcgatcgcg acctcgctgt
     3661 cgcgtctcgg cgacgtgcag caggatcgcg gccagtacga gtccgcgtac aactgccaca
     3721 aggatgcgta cgagctgcgc aaggccgccg gcgatcgctg gggctgcgtc gtctcgcaga
     3781 acaacctcgc tgccctcgcg ttcgagctcg gcgatgcagc agaagcgcgc gcgacgtggc
     3841 tcgccgcact gcccgagtcc gaagcgatcg gcgcgctccc actcgaggcg ctgatcctga
     3901 cgaacctcgg cgaggtcgcg ctcgtcgaag gcaagctcga tgaggcgcgc acccggctgg
     3961 agaatgcgct cgagatcgtc gaggacatcg atgatcgcgg cctcgagagc gagtgctgcc
     4021 gtcacctcgc cacgctcgag aaatcgcaag gtcacccgac ggccgctcgc gagcatgccg
     4081 agcgcgcgct cgaagttgcg aagaaggcgg gcctgcgcga gaaggaagca caggcgtaca
     4141 tgacgctcgg cgatgttctg tcggcgagcc tctacgacgc cgaggacgcg cagagcgcta
     4201 ccaccgatgc ggcggttgcc tacggtaagg cgatcgacgt gctgaagcag atcggcaacg
     4261 aggctgctct cggcaaggcg ttgttcgcat tcggccgcta caaggccgag accggagcga
     4321 tcgccgccgg caaggacatg ctgcgcgatg cgctcacgct gttcgccaag ctcggcctga
     4381 agcgcccgca cgacgacgca aacaagctgt tgtcgacact ctccgcgtag acatccggct
     4441 ccgcatgggt catgtttcgc agacccgcgg atgatctggt tctggctagg gttcttcggc
     4501 ctcgtcgcgt tgttgctgtt cctcgatctc ggggtgctgc atcgcaagga tcaggagcag
     4561 tcgctgaaga ccgcggcgat gtggaccgtc gcctgggtga tcctcgggct ggcgttctcc
     4621 gccgtcgtct acctcatcta cgaaaaccat tggctcgacg ccaagctcaa gcacacgccg
     4681 gtcggtcacg ccgacggtgg tgaagcggcg gtcacgtaca tctcggcgta cctcctcgag
     4741 caagcgctct cgatcgacaa catcttcgtc atcgcgctga cgttcgcgca gttcaaggtc
     4801 tcaccccagt tccagcaccg cgtgctgttc tggggaatcc tcggcgcggt ggtgttccgc
     4861 gtcgcgatgc tcggcggcgg cgtctggctc gcgagcaagt tcgattggat cttctacatc
     4921 ttcggcggct tcctcgccta caagggcgcc gagctgctca aagggttcgt cgcgaagatg
     4981 cgcgcgaagg gcgaagagga agaagaagag agcgagctgc cgtggctcgt gcgcaagcta
     5041 cgccgggtca tccgcatcga agagaatcac catggccact tcgccgtcga gatcaacggc
     5101 aagaaggtcc tgacgatcct cgcgctgtgc ctcatcacgg tcgagctgca ggacatcgtg
     5161 ttcgcgctcg actcgattcc tgcggtgctc tcggtctcga acgagacgtt catcatcgtg
     5221 acgtcgaaca tcttcgcgat cctcggcctg cgctcactct acttcgtgct cgccggcatg
     5281 atggcgaagt tcgaatatct cgagctcgcg ctcggcgtgc tgctcatcgg catcggcgtg
     5341 aagatggtgc tgcacaacca cgtgcatatc tcgcacatca tctcgctgct cgcgatcgcg
     5401 ggcatcctcg cggccggcgt gatcgcatcg gtgatccgca tgcgcaacgc tgcaaatcag
     5461 cagccgccta tcgaaccagg accagcatct ccgtctccat gatctcttcg gcccagcgac
     5521 gcttgtagtc catctctgtg ccgaggtcgt agcgcgcgat gccttcgctg cagagctctc
     5581 gcacttgctg cagctgcagt aagccgccga tgccgagctc ggcgtagccg tcgtcgtagg
     5641 aaaactgcag gccgcgatac tcgtcgccca tcactgcgcc gaggatgtag ccgatgtctt
     5701 gcccatcgag ctgcgcgaag atcgtcctct gctgaccgag ctcgcacagg cgcggcagca
     5761 tctggccgta gaacgcgcgc atcggtcccg catgaatgcc gacgccctcg tgcgatttcc
     5821 agctgcgcgc ttcgaccgct tgaatgcgat cgtagagtgc cagtgcttca cgctgcgttg
     5881 cgcgcaccga atcaaacgtc atgccgcgct ctcgtgcagc gcgctctgtc ttgcgcagcg
     5941 atttgcgaag ctcgcgcgaa cggcgtgaca gaaatccgtc gatcccgccg tcgaggctcg
     6001 cgacatggcg aatcgtcggc tggccctgcc tgcgttccca gcgcgcggga agtgtggcgt
     6061 cgagggcacg ccgttgcggc ccggacacgg tgtgacccga cacgatcgcc aactgccagt
     6121 cacgcctcgc ggcaaggagc ggcaccacct cggcgattag cgccgccgca tcctgtccga
     6181 tgaaaggtgc ggcaagcccc cacgcgagct cgaccggctc caaatacgga aatccagcag
     6241 gatggacgcc ccgcatcatc gccaagtacc cgctcttccc tcgaaacgtg aacgacgcac
     6301 gaggcggcat cagcgctgcc gaggccgaca gaatccacgc gctggccgaa cagaagcgat
     6361 ctatctcagg cgtgagcgct acttcgcgat cgaagtcatc ggcacaggcc gcgaggtcga
     6421 tcacgtccac tgcgcacatc gtaccgcgtg cacggtttct gcaataatgg cccggatgcg
     6481 ccttgtgtcc ttggtggtgg ccacgacggt ggcggccaca ggctgtgcca cgactagtta
     6541 caagattccg acgtcggagc tgaatcgcat tgcgagccag ccacccgaga cgcgcggcca
     6601 acacgttcgc gtgatgcagg agctcgacga caccgacgcg ggcccgccgc agccggtgac
     6661 cgccgaaacg cagatcatct tcttcccgca gatcaacgtg tacggcccga gcgaacggcg
     6721 tcgctactac cagacgaaca gcacgtgggg ctgcggtggt tgcggcggcg gtggcgtcag
     6781 cggcggcggt ggcggccaca agagcggcgg cggcaaaggc ttcaacttca acggcggcgc
     6841 aggtgacggc aaaggcgcag cggtcgcggt gctcgtcgcg gccgcggtcg cgctcgtcgc
     6901 cgtcgcagca gtcgaaggct cgcgcttcga tggcttcgcg aacctccacc cgatgcatcc
     6961 cgtctacatg atcggcaaga acggcgagca catcgtgctg ccgctcgcgt ggatcgatcc
     7021 cgaaaccgca aagtggacgg acaaggcggt cattcgctcg acggaaggac cgtggcagcc
     7081 tctcgagcgc gcgccgctcg atcgccaagg ctggacctac gcgatgtacg gtggcgtcgg
     7141 cacgttcgag tccgccgacg gatcgaaaga caacggcacc gcgacgacga tccagctcgg
     7201 ctacttcccg acacacgagg tcggcatcgt gggctcggtc ttcttcggct ggcgacagaa
     7261 cgccgtcgac cagacgctgt tcgagtcgcg ctacacgctc gagctgcagg gctacccgat
     7321 ccatacgggc ccgatgcagt tcggcggcta catcggtggc ggtgccgcgt atcgcttcga
     7381 agacggcatt cagggcggca actccggcag ctccgcgctg ctcggtggcg cgatgatgca
     7441 gctcgatatc aacacgcggc tcgcgctgac cgcacgcttc ggcctgacgc gcgcgcacga
     7501 agagcgcatg accgacgcga tggtcggcct ctccgtctat tagctagtgc ccctcgtggg
     7561 gcttcttctc gtcggtctgg tacttcgctt tccactcttc gtacggcatg ccgtagatcg
     7621 cctggcgcgc ttcggggtcg gacagcgcga cgccacgctc ctcggccgcg gcgcggtacc
     7681 acttcgacaa gcagttgcga cagaagccgg cgagcttcat cacgtcgatg ttctggacgt
     7741 cggtccgctc ttggaaatgc ttcaccagac ggcggaacgc ggcggcctcg atctcaatac
     7801 gtgtcggttc tggaatgtcc gtggccatgg caggtggata gcacgtggta tacgcgccgg
     7861 gtgaccacgg tcgaacacga gggaacgacg tactacatcc tcgggaccgc tcacgtctct
     7921 cagcggtctg tcgacgaggt ccaggctgcg atcaaagagc tccagcctga tgtcgtgtgc
     7981 gtcgagctcg atcaggcgcg ccacgacgcg ctcatgaaag acagcgcgtt tcgcgacctc
     8041 gatgtcttca aggtcgtgcg cgaaggcaag acgctctacc tgctcgcgca cctcgcgctg
     8101 tcgagctacc agcgcaagat cggcgcgagc ctcggcgtga agcccggcac cgagctgctc
     8161 gctgcagtga agaccgcgaa cgaagccggc attcccgtcg agctgatcga tcgcgacatc
     8221 aacatcacgc tcaagcgcac gtggaagaac ctcggcctgt ggaagcgctc gatgctcatc
     8281 agctcgctgc tcgtcggctt cgaggatgat gaagacggcg cgcccgtcac gagcgagacc
     8341 gtcgagaatc tcaaggagcc caaagcgctc tccgagatgc tgaccgagct cggcaaagcg
     8401 gtgcccgagg tgaagggccc cctcgtcgac gagcgggatc agttcttggt gagcaagatg
     8461 ctcgaggccg gccgcggcaa gaagaaggtg ctcgcggtcg tcggcgctgc gcacgtgccc
     8521 ggcatgctcg agaacatcaa cgtcgcgatc gatcgcgaga agctcaacaa ggtcccgccg
     8581 ccgtcgctga tctggcagat tctcaagtgg ggcatcccgc tcgcgttcgt cgtcggcatg
     8641 atctacttct ggcagaccag cacgacgacg acgctcgcgc agatgatgct cgcctggatc
     8701 ttgccgacgt cgatcggcgc ggcagggctc acactcatcg ctgggggatc gctgctgacg
     8761 gtgctcgccg cgttcatcgt ggcgcctatt gccgcaattc acccgctgct cggaacgggc
     8821 atggtgaccg gcatcgtcga ggcgtggcgc cggcggccaa gcgtgcagga ttgcgagcgc
     8881 ttgcccgacg acgtgcagag cttcaaaggc ttctggcgaa atccggtcac gcgaattttg
     8941 ctcgtcgccg tcgcatcggg gctcggcacg gcagtgggct tctgggtcgg cgtcggctgg
     9001 gttgccagtc tgttgtgact atcgtcgtgg actttgaagg tccgaccacg ctaaacccgc
     9061 actgatgatc aatcgcagac aggcgatcaa gcgcatcggt gggctcgctg gtttggcagc
     9121 gatgccgaag atgctgtcgg ggtgcggcga cgatggaggc tccgacacgc ccggcatcaa
     9181 gaactacgtc tacatgatgc tggagaaccg cacgtacgat cacgtgttcg gcgcgcgcaa
     9241 gctccagggt ctcggcggtg atggcctgac gaccggcatg gcgctccccg acatgaacgg
     9301 cacgatgatt ccgatcttcg agccgagcgc gaacgagatg tgcgtcgccg atccgccaca
     9361 cggctgggat ccgtcgcacg cgcagttcaa cggcggcgcg atggacggct tcattcgcca
     9421 gtaccagatg gcggagccgc aagcgccggc gaagcaagtc atgcagtaca tgacgcgcac
     9481 gcagatgccg gtgagctggg cgctcgcgga cgagtacacg acctgcgatc gctggttcgc
     9541 ctcggtgatg ggccccaccc tgcccaaccg cgcttactgg catgcggcca cctcgttcgg
     9601 cctgaaggtg aacaacgagg tactcacgca gttctcggcg ccgccggtgc cgacgatcta
     9661 caacaagctc cacgacaaga acgtcgactg ggtctactac tccggcccgc tcgcggtcgc
     9721 gtcgctgctc ggcaacccgg gcccgtacat gctcgacctc ggtccgacgg acggcaccgg
     9781 tcacatccgg cggttctccg cgtatgcgga tctgcccgac gatccgaacg gccagttctt
     9841 caaggatgcc aaggccggca agctcgcgcc ggtcacgtac atcgatccgt tcttcggcga
     9901 gaacgatgac catccgccgc tgcacccgat catggcgcag gcgctgatcg ccgccgtgta
     9961 caacgcgctc gcgcagtcgc cgcagtggaa gcacacgatg ctcgtgatca cgtatgacga
    10021 gcacggcggg ttctacgatc acgtcgcgcc gccaaagaca acggacacca ccgaggcaga
    10081 cttcggtgtt tccggcttcg accagatggg cttccgcgtg ccggcgatgg tgatcggtcc
    10141 gtacgccaag aagaactacg tcagcagcac ggtctacgac catacgtcgg cgctcaagca
    10201 cctcacgaat gtgttcggcc tcgagtcgct caacgcgcgc gtcgatgccg cgaacgacct
    10261 catggactgc atcgacatgg atcgcctgat ggcgggtgac ccggccgatc cgatcgatct
    10321 gccgacgatc gatctctcgc agtacgcgca gtacatgacg gaccccgact gcaagggcat
    10381 ggcgttccgt ccactcgatc ccgtcagcga atgggccgat ctgaacccgg gcatcatgct
    10441 ggattaccgc agcccgaatg agcgcaacgc gtatcaccgc gggatcatga aggcgctcga
    10501 cgagcagcag cggaacagag cgttggtgaa atcgcgttga ccccgccccg gcctaggggc
    10561 acactttccc aatcatgaag aagctctcgc tcattatcgc tgccggctcg ctcgctctcg
    10621 cgacgcccgt gttcgcctgc ccgggtcacg actcgaacga gacgaagacc gcggagaagg
    10681 cccctgcgaa ggaagagccg aagaaagagg agcccaagaa ggaacaggct ccgaccacga
    10741 cggccaaggc caaagaggcg cccaagaagg aagcgcctgc gaaggacgct ccggcgaagc
    10801 cgaccaaggt ctcgcagaag taagttcttc cagttcgctg cgttgacggg acaggtcgta
    10861 agatgatctg tcccgtcttt tttcgtttcg gccgtttagg ggtcatgagg gcgctgctca
    10921 tattggtcgc gatgtgtgcc gttgcacgcg cgcagcagat gccgtgcgag ccgtgtgttc
    10981 gcggtgacga cacgcttcgc caatggggcc cggccggcga ggagctccgc accaaggtgc
    11041 aaggcgtgac ctcgctcggc attggcacgc tggagatgaa cgagcgcgac gcacagcagc
    11101 tcgacatcta catcggtgcg cttcgcgagc cattgcgcgc gcggatccaa cagctctcgg
    11161 acacgcagct gagcgaagtc gcagcggcac tgtgcaacgc cccggacggt ggctgcgtga
    11221 cgaacatggt gatcgcgatg cagtgcattg ccgagcgctg cacgatccga cacgagcagc
    11281 acaacgacga gcgcatgctg cccgtggatc cacccggact cccaacgtgc gatccggtcg
    11341 ccgacaaaac gaagtcatcg aaagctgcga tcggtttcga atgggcgacc ggctggcagg
    11401 acgatgcgtc gccgaccgat caccgcgcgt ggtccctcgg cttcgaagcg cgcacacgcc
    11461 tgaacaagcg cgcgggtctc gtcggtcgcg tcgatcggtc tacgggccgc gacaaagcaa
    11521 acgatgccga ccgcgatggc cgcgatgatt tcggcaccgg gccggtcacg cgcgtgttgg
    11581 tgctcgccgg accgagctac gcgtttgcgg tcgcacacga caaagacatc gtccgctacc
    11641 tccagctcga cctcctcggc ggctatcagt ggacgctctc gaaggaggac gaagatggct
    11701 tcgcggcggg cgccgatctt tcgtatacgc tcgcggtggc gcgcgtcggc atgcgcttca
    11761 cgcaaggctt cggcgatgcg gaggatgcac gcgcggtgct agcgcacgtc ggattcgtcg
    11821 tcggtggtgc gccgccggtc gactacggct cgggctgcga ccgaccatct cgcacaccga
    11881 cgaagctcgc gctcgcgctc gacatcccgc tgttcggcta cggcatgtcc gaccagctcg
    11941 actacatcgt cccgggcttc ggggtcgaag cgctctatca cttcacaccg tcgttcgacg
    12001 gcttcgtgca cggcgacatc atcgatgcgc ctaatggcgg acgagatcgc gcggtctatc
    12061 agacgctgct agccggtgca cgtctcgatc tcgcgagtcg cgatggcgag cacggcacga
    12121 agacaggcgt gttctcgacg ctctccgccg gctacacgtg ggcggccaca accgattcgt
    12181 cgacggcggg ctcgggcccc gttgccgagg cagcgatcgc gtggggcggc caaggcgatg
    12241 acggcggcgc gtatctcaag ctgcacgcgc gctttggcct cgtgcccgac aactacgaca
    12301 tgcgcgcgct gtttctctcg ggcggggtcg agctgcgtct cgatcgcagg aagtggcgcg
    12361 accgaatggg cggcaagaac gattagcaga ggatgtcgag aagctgcgcg cgcgtgaggt
    12421 agccctcgag cgcagcggca aactcgtcga tcatgagatc gagctcgagc gtcatccgct
    12481 tgccctgcac gcgatgcacg cggcccgaga tcggaatctc ggtaccagcg agctgcgcga
    12541 cgcccgacac ccacgtgccg atcgccggtg gctcggtcgc gaggctgacc gtcaggtgcg
    12601 tgcgcgagat gtctgcgatg ggcgacgtcg cgctatcgaa cctgcacgtc gccgcgagcg
    12661 ccggcggagg ctcgacgcgg tagttcacgc ggcggttgtg cgtgatgagc ttgtcgagcg
    12721 tgtcgtgcac gggcgcggag atcggcgtct cgggcgcgtc gagcacgagc tcgcgcaggc
    12781 gcttgagctc tttctgcgtc gtgatgaggt tcgtgcgcat gtcggccatg cgtcgctgat
    12841 cgcgatgcac tttgtcggcg accgtctgca acagattcac gaggaagcga acaccgagct
    12901 cctggttgtc cgcgaggtag gcctgcagct tcttcgcggg cagcgcccac acgacggagc
    12961 caggcgtcac gttcgcgcgt gtcgaccgcg agaggcccgt cagcgcgccg agctcgccga
    13021 tcagcgccgg cgggtgcaga cgaaacacgt catcgcccgg acgatcgagg acgacctcgc
    13081 ctttcgttaa cagataaagc gtcgtcgctg gctcggtcag ctcgaacagc ggcttgtcgc
    13141 tggcctcgac ctgggtgaac aggcccgcga cggtgttcag ctcggcatcg ccgaagcctt
    13201 gaaacaacgg gatggttcga agctcgctgg cggtcggccc catgccgcgg aacataacgc
    13261 ggtatcgtcg tggggtgagg gtctggacgc tcgttctgct cgtcgcgtgt ggcaaggatg
    13321 ctgggcccgc gaaaaagccc gaagctgcgc gcaaggacgc cggagctgcc gcagctgtcg
    13381 cagctgacgc agccgggcct tcggtcgaag cggactcgtt tgccgacctc ggcatcgcgc
    13441 tcgagacgat catccctccg gatgcgcgcg tggttggctt tggcgagctg cacaaccgca
    13501 ccgaccgcgc gcaggtgaag tcgtcgctcg cgcacttcac gcaggaaggc ctcccggcga
    13561 tctccgacaa gatgtcggac ctgatcgtcg agacgtgggt cgtcgatccg aagtgcggcc
    13621 agcaagcgaa gaccgcgacc gcgaaggtcg agatcacggt acgccgcccg caggagacga
    13681 agtccgagat cgcgttgcta gccgaggccg cgcgcgccgc gaagatccag ccgcacgcga
    13741 tgaagatcaa atgcgacgag tacgacaaga tcgcgccgca gggcaaagag gtcgcgcccg
    13801 acgtgctcct cgatctgacg acgcgcgagc tcggccgcat ctccgaagag gccgtgaagc
    13861 atcgcgacaa agagcccgag caccggccgt ggatcgcgct ctacggcggc gcgctccaca
    13921 acgaccgctt cccagcgaag ggcgtcgagt cgtggagcta cgcggcgaag gtcgatggcc
    13981 tcaccgcaaa tcactacgtc gaggtcgacc tgatcgtccc cgagctcgcc gagctcgatc
    14041 ccggatcgca aagcgccccg tggttcccgc tcgtgcaatc gaaggacgac aaggtcaaag
    14101 tcttcaagcg cggcgagcgc tcatacgtca tcgtgctgcc gaggacgccc ggatgatcgg
    14161 cgcgatcgct gcgcgcattg cggaggcgtg gctgctctgc gcgatcctgc agtgcgtgct
    14221 gtggttcgtc gccgagaaga cgaagaatgc gggcatcgtc gatgtcggct gggcgcagtc
    14281 gttcaccgcc gtcatcctcg tgttcgccgt ccagccgctc acgccgcgca gcaactggct
    14341 gccgatcgcg atcgtcgtga tcgcgtggag cactcgcctc acctgctatc tcatcgcgcg
    14401 aggcgccgcg accggtcgcg aagaaggccg ctacgtcgac ctacgcaggc gctgggcgct
    14461 ccacgcatcg aagcgcttct tcgtcttctt ccaagcgcag gccgcgctcg ctgcattgct
    14521 cgcgaccgcg ttcgtcgtgc cgttcgtgtc gcccgcgtgg gacggcggca tcgtgcgcgc
    14581 attcggcgcc ctcgtcgcag cggcgggcgt cgccggtgaa gccgccgccg atgcccagct
    14641 ccgtgcgttc aagaaggatc cggcgaacgt cggtaaggtc tgcgacaccg gcctctggag
    14701 ctactcgcgc catccgaact acttcttcga gtggctcgtg tggctaggct tcgcgctgta
    14761 cggcctcgca ttcgctccag ccggcctcgt cgcgctgctc ggcgcagcga tcatcttcgg
    14821 ctcgatctgg ggcgttacgg gcattccgcc gacggaagcg caggcgctcc gcagcaaggg
    14881 cgaggcgtat cgcgactacc agcgccgcgt cagcaagttc atcccgctgc cgcgcaagta
    14941 gtcagggcca aggactcgtg cacggcccag gctcgcgcaa caagctgtct ccgtcgcatg
    15001 cgcgccatta tccaggtgac ggcctcctcg atacactcgg gcgcgccatc tgcgcggtcg
    15061 actgcctgcc acgcaaggag ctgcacgagg cctgggagat ggccacccgc atacgtgcct
    15121 ggctgccgtc caccccgacg gggcgcgtgg tcgacctctg cgccggatac ggcctgctcg
    15181 ctcaagtcct cctgctgctc gataccgata tgcgatcgac ggctctcgcg gtcgacatcc
    15241 gactgccgaa gaaccacgtg atcgtccacg acgccgtcgt cacgacgttt ccgagcctcg
    15301 ccggccgtgt gacgttccag cgcgcgcgcc tcgaagatgt cgacgttgga gtcggtgacg
    15361 tgatcgtctc ggcacatgcc tgcggagcgc tctccgatgc cgtgttgagt cgcgcgtctg
    15421 cagtgtgcgc acgcgtcgct ctgctgccgt gctgtcacct gacgaggtgg cgtcccgatc
    15481 tcgtcgatcg tgccgatcct gccgcacgga tcgacgaaga gcgcgccatg tggctcgtcg
    15541 agcgtggcta cgacgtgatc gtcgattcga tcccagccaa cgtcagcgcg aagaatcgcc
    15601 tgctgctcgg tcgaccgcgc gcccacccac accgagccga gccggcaata gaacgcggcg
    15661 atgattcatt gcgtcgctct cggtcctgac aacgcggtct actcgacaag ctcgacgccc
    15721 tttgggtatc gcctagcggc attccggccg cctgccagcg cgaacgcagc ggcgacgatg
    15781 cggtagctca ccgcagcagc tagcccgtgc tcgtctgata atgtcctcgc tgaacggaca
    15841 acaagatgtt caagcggacc gtcacgacga tcgtcctcgg cgcactgctc ggagcgtgca
    15901 ggcccaagag ccctcactac gaaaccgggc cgtgcgagga gagcatccgt gcgcgcgccg
    15961 ccggcaagta cgtgaatcag acgcatgccg aggcggagca gcggaactac ttctacttcg
    16021 cgtcctgcaa cgacctctgc gacgacgacc acgagcggga atgtctcgag cttggactac
    16081 tcgaggacga cttcggcgtc aatccggccg aacccatact gagcccgctg tgcgcgaagg
    16141 ggaacaaggc tgcctgtgac tgggtcgcag cgaacccgga gaaagtcgcc tcgtcgcaga
    16201 actacacaaa ggtggtcctc gcgcgccggg ccgcgggcag cgatgagcgc ccgacatcac
    16261 aatcggacct ggcatcgttc gccgcgcagc tgaagcgcgc tgcgatcggt ttttccacgc
    16321 tgcgtgacga gactttgtcg ctcggtggcg gttacacgac gttcaagatc aacgtcacgc
    16381 tcggtcgcaa gtacctggtc gtgtgtgcgg tcgaagatcc gtcggtcatc accgctcgaa
    16441 tggaaagcgg cggctacacg ctggagctgg agcccggcca caaccagctt cccggagtct
    16501 ttaacgttca gctctcgttc gaggccgggg acgcgggggc ctccgcgaag gttttcgtcc
    16561 ggtcggcgag tggaccgcag cgcagtgtcc gctgctttct cctcgagaag gactagcagc
    16621 tcatggatga cgacgatctt ccgcggttca gcagtttcag cgttagggcc cgacggtacc
    16681 gtatgtgatc acttcgtcgc gcatcgatgc gacgtcgtat gcgatccagc tagcgctgcg
    16741 cggcaccgtc tcgatgcgcg cctcgtcgat cattccgtca aagaaatcgg cgtcgggacc
    16801 cgcgcccgaa ttcgcgtcgg caccgagcag gaccggatta ccggaggcga acagcgcacc
    16861 gccgatcacc gacgtgacgt tgcgctgcat gccattgatg tagaaccgtt cggtggttcc
    16921 gtcgtacgtc atggccgcgt gcagccaggt gccggccgtc agcgttccgc cgccgccacc
    16981 ttggttgaac gtcgacgctg tcccgagctc ggcgtaggcg attcgcgtcg agtcttcgat
    17041 gccgatccaa aacgcttcgc tggaacccat cgcttcctgc gtgaccgccg cgttgtagcc
    17101 ggatgccgcg acgaacgatg aggggttcat ccagcacgag accgtgatgc cactgccgaa
    17161 cgcgaatgca ggcgcatccg cgaccacgat cgaatcgctg tttgtcgtcc tgaacgtgtg
    17221 tgcttttgcg atctgcccgt cggagacagt ttcaggaccg ccggaccacg taccggctag
    17281 tgcatgctgg gtcgagtcat tgcgatcgcc gaggtgatag acggccgcgt acggcgctgc
    17341 ccagaccgac tgcgtcgacg cgggcggcgg tgtgccgtgg ccgtaatcga tcgtcagcgt
    17401 cgtcgggccg tcgatgatcg ggacgcgaac ccacgcgacg agcggggcgc cgccggcaga
    17461 gccgacttgc tcgatctcgt gcggcaacag cgtgcccgcg ctatcgaaga accgcagatc
    17521 cgacgcgtct ggttgcatgc gatcgcgtgc ggcgcgggtg tcgtcgagca cgacgagcag
    17581 cggaaagtct gtgagcggct cagccggagc cgcgagcgtc agctgcgagc ggagcgttgg
    17641 gacggcggcg tcgctgagcg gatcgaacgc gatccgaccg cacgcgcacg tcatcaccag
    17701 cgcgtgcgcg attcgcatcc ccaagaaggt atcatcgctg ccatgcgaat catggctctg
    17761 gtggtgctcc tggtggcggc gtgcggcagc aagaagagcg ataacgcggc cccgaaaggc
    17821 gcgaaggtga tcgagtggga tgcgaccaag agctcgcgcg cgacgtacaa gaccgcgacg
    17881 ttcgacaacc gcgtcacgcg cgatgacatc atcagcgtca aagcctgcgc cgacgagatc
    17941 aagctcaagc ttcacgtcga caccgcgacc gcgaagtaca cggaggacgg caagcccgtc
    18001 gagattgtcg cgctcgcgtc gctgacggcg accgtcgagg atggcaaagg cttcgagctg
    18061 acgaacggcg cgtgcgacgg cccgaactat cagctgaccg cgcctggcga gccgcccggc
    18121 tacacgatcc tcgactgcca cttcaaggcg aagaaaccga acaacgactg cgcgccgatg
    18181 ttccagctca agggcgacgg cacgctgctc acgaactaga acgtgatccg tatcggggcg
    18241 gtgaccttcg cgcccttgcc gagttgaagc ttcttgatga ccgcgagcac gcagttgcgc
    18301 gtggtcgtgt ccttgagcgt cgacgatgct gcgatcttgg cactcgttac cttgccgttc
    18361 gcgtcgaccg tgatcgcgat ctcgagcgtg cccgtgccgg ccttgtggca ggtcgtcagc
    18421 gcggcgcgct gcgcgtcgag tgcggtcttc acagcctcgt ccggggtgtc gaatgcgagc
    18481 gctgcttcgc cgccggcttc ggagccaccg acgagggctg gctttgtctc ggcggtctta
    18541 ccgccggtct tgcccttcgt ggttgtgggc gtcttgtcgt ctttgagcgg cgagtcgtcg
    18601 aacctgtctg ttgcgccgag catgccgttt gtacgcgctt gctgcttcgg gctgtttgga
    18661 tcggcgtttg cgctcgggtc gagcggcttg cctccttggc taggaccgcc aggtggaatc
    18721 gcgcccgtcg tgttcggtgc ctcgttctgc ggcgattcgg tcgtgaccgg cgtttggtcc
    18781 gctggcgacg gtgttggtga gccacccgtt gcgcccgcac cgcggtcggt ggtcgctttc
    18841 tcgctgctct ttgcggcgca ccctgcgacc aacgccaacg cgaaaacgag acccgtgaag
    18901 cgaaccatgc cgaaggtata ccgcgccgcg accccagatt caggtcgagc tgagatcgac
    18961 cattagctcg ctgccgagtg cctcgagacg agcgcggagg tcgtcgagcg cgacgccgcc
    19021 cggagcccgg atcagcgcgc ggtagttgaa gagcgggccg ccggacatcg ctgcgctcgt
    19081 gacctcggtc tcgaggtctt cgacgttgat gcctcgctcc gcgagcacgc gcgacacgtc
    19141 gcgcacgatg cctgggcgat cgttcgccgt gacggtgagc ttgagcacgc tgtggtcggt
    19201 tgcgggctcg ggtgcagcgg tgcggcgtgc ggtgacctgg agtccagccg catcgagggt
    19261 gcgtagcgac gcgacgagct cgtccgtctt tcccgcttcg atcgtgacga gcaggatgcc
    19321 ggcgaactgg cccgcgagcc gcgccattcg cgcttcttcc cagttggcgc caagcgaaga
    19381 cagcttctcg gcgacggact ggacgaggcc ggtgcggtca ttgccgatga gcgtcaaaac
    19441 cagggtgtcg cgcatggggc gaccatacta cccgacggtt catggtgtgg agcggccgcg
    19501 cgattaacgc tgtctaggtg attcgttctc ccgagcgcgg aagcgcaatg ctcgtcacgc
    19561 tgatcatcat cacggcgctg atggcgggag cagcgacgct aacgcagatg cagctcacgt
    19621 cgacgaggag ctcgagcctc gtgcgcgaca agatggcgtc gctgcactgc gcagaagccg
    19681 ggctcgccgt ggcacgcgcc gcggtcaccg cgaacgtcac gagctggaat tccgcgctcg
    19741 cgatgaccac agagccatcg tggctgcagt cattgccgca cgatatcgac ggcgacggcg
    19801 tccgcgactt cacgctgcgt ctgcgcgaca acgccgacga gacggtcgat aacccgaacg
    19861 tcgataacga cctggcagtg ttcgtcgtgt cgtcgtgcgt gaagttcacg gacgccccgg
    19921 cggaagtgat ggagctcgtt cgcttcaacg gcgggggcag ctgctatcag gcgcagctcg
    19981 gcggctgcgg aggtaactcg aatgcgaact gagctgatcg ttgtcacgat cgcgattctc
    20041 gctgcgccgt cgatcgtcga cgcgagctgc gtgcccggct tcgacttcgc gatctttgca
    20101 aaggacacgc tccaaattca gggcaatgcc ggcactgacg cgtggaattc cgccaccggc
    20161 acctacgcag caacgaagac atgcgcgaac gcggatatcg gaacgaactc gtcgacgtcg
    20221 ggggccgctc acatccagag caactcgacg atgatctgcg gcgacgcgta ttccggcgcg
    20281 ggcagtaacc cgaccgcggt ctacaccggc
//
LOCUS       DATLBZ010000136        25374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6028781, whole genome shotgun sequence.
ACCESSION   DATLBZ010000136 DATLBZ010000000
VERSION     DATLBZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 25374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25374
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6028781"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /locus_tag="VMZ53_07315"
     CDS             <1..1670
                     /locus_tag="VMZ53_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS28299.1"
                     /translation="FRAALESDADFEQLPGERPAQLRSRDDGAALLRRLLTLSRRLNS
                     EESVERILDEVIDTAIELTAAERGFLLLRQADGELAPVVSRNFAMGDLESGERSVSRS
                     IAERAAQTGEPVVTVDAGVDERFAVAASVAALRLRSVLVVPLRQRAAITGCIYVDHRL
                     RGGAFDDSAASLLGELADIAAIAIENARLTDDLRKTSREVETLNRRLAAELADRDAEL
                     VRVKADLPSRDRLRNAYEGIVGKSPAMVRLLDTVDRAAATSLPVVIVGESGTGKELIA
                     RALHDHGPRRDGAFVAINCSAVPEPLLESELFGHVRGAFTGAERDRRGLFEVADGGTL
                     FLDEIADTSPGMQAKLLRVLQDGMIRRVGDSKTRKVDVRVVAASQRSLAELAAAGTFR
                     EDLRFRLEVITVPVPPLRERDGDVPLLVEKLLALLCRGRTPPKITRAAMRALGQHRWP
                     GNVRELENALARGVALGGDVIDVADLPEAVAASASRPEPAKPAVGEDLRLKPALVATE
                     QSYIGAAMTRAKGNQTVAARLLGLSRFGLQKKLRRLAGEDDGSSDDE"
     gene            complement(1704..3500)
                     /locus_tag="VMZ53_07320"
     CDS             complement(1704..3500)
                     /locus_tag="VMZ53_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28300.1"
                     /translation="MRFCVALLLLLAGCGRIAFDARDDAAPADAQELGLFTPVALPPG
                     GDVNNVALAANGDVFVALPYHGMYRSRDRGVTWTPCGELRTRHVAIMGSDTIFASDDP
                     GDVFVSDDGCAHWRSTELHIYTHELAVLGSTLYAGTDDGLRAWDGAQWRTISTPFEGQ
                     AITGLSLQPSLMLIGSQVGGIARSIDNGQSWSVSNTGLQNLDVGAIRIAPSNPMRAYA
                     VEVTAGGTTGALFSSVDGGVTWTNIYANGGFNVAVDPNDPNHFLFANYNSLFESTDGT
                     GTVDIRSSVMQGSVPHDAVFDGMGGQYIATSRGVFFRQGTGPFEARHTGIAGWTLDRV
                     ALTPSRDGVYWATPTGALVSNDGGKTYNLVWELGGFASDINGIVVPGQHEASVVVTGR
                     HVSVSNNRGNTFAAVFDTGAANGFGAYDVASLSSGRLIVVTRTQALYADPPWTAWTAV
                     LVGTGPQNCYQATVLTNDDVVIACDEGLFRSTNQGASFQPLPAMSGLEKPFAGPGGLV
                     LAGSEDGLWSLEGTWQPHGLAGIHVRDLWIEDTTWIAATSPGVYVSHDSGVTWTRLAG
                     LVGKTPMLLARDVDGYLLVAIAGDGLYRSPFP"
     gene            complement(3502..4530)
                     /locus_tag="VMZ53_07325"
     CDS             complement(3502..4530)
                     /locus_tag="VMZ53_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS28301.1"
                     /translation="MARIVVLYNTDYDEEQAANAGNPDATSVRESAKAIARALMETGH
                     TVELTGVHGIEVYDVLARIGRDKPDLLFNLCESMDGDPRNEPTFAGLLDLFHIPYTGA
                     DLLALASCLYKQRTKDILLAQGVPTPPYRYLRTPADLDDASLEKLDYPWFVKLAHEDA
                     SLGITEANVVRSAADLRARSRELMAEFHEGVIAERYVEGREVNVTLFGSGEHVEVLPL
                     HEIDFAAMPSDRPKIVSYAAKWDEKHVDYAGTKPVPLRGLSDAVRAEIERVSRAAWAA
                     VELRDYGRVDLRVDDAGVPWVIDVNPNPDISPDAGVARAAAAAGMTYPQMVDRIAQLA
                     LVRIRLHS"
     gene            complement(4537..5172)
                     /gene="lipB"
                     /locus_tag="VMZ53_07330"
     CDS             complement(4537..5172)
                     /gene="lipB"
                     /locus_tag="VMZ53_07330"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HUS28302.1"
                     /translation="MLAAAPSAAALEWRWLGRADYRPVLDDQLAERERVWAGAPGTIF
                     LCEHPPVITLGRSANTANILAADGVQIERIERGGDVTYHGPGQLMVYPVVRVQHGVVE
                     FLSRVAAALADACAALGAPGAEFHRDPAGIYYGDNKVGACGIHIARGVSVHGFALDVA
                     TPPDAWRKIRPCGLSLPQTSVVRELEARGLPAVSVEQVAREAGPRLVAALL"
     gene            complement(5172..6641)
                     /locus_tag="VMZ53_07335"
     CDS             complement(5172..6641)
                     /locus_tag="VMZ53_07335"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HUS28303.1"
                     /translation="MAKKKSSGDAEGDAEATKTKKKTAAKKPAAKKAAAPKKSRSKKK
                     DADESENAVVDTAPEVADVIEAEIIAETPASAMADSTDENDAVEPPTALSEEEQELSA
                     IYGDDLTKPATAHREFQDNKTRDEDRPMMPEINARDERKARWEDRRERRRRERDERRA
                     RREGGHPGQRPPQEQRDGQGRDGQSRDGQSRDHRDNREPRPQGEPRPPQQFQQAAPQP
                     PRPSAPQINGHEHDQLARVGTPLGDAASAVFQQLRNGQPLPVRQLAAMMRKRSLVNQD
                     PEQLWPQLKAELLGDERSYRTLGLRPRIVYRGRDLFSPGPVSMSATADAEAGLASALS
                     KLASATHRALSQRLAKASPAGFERIIHAYLVAAGYRDIDWVKRIGGISYATAIAPGLD
                     RTVLISARSGSEPIDRRGVGELRVGVEAKDLVTGILITPGELSEDAERELERAGRSIS
                     VVCGDQLVAALIQAGVGVVSAAAPLHYLDDQLLEELLAG"
     gene            6756..7754
                     /locus_tag="VMZ53_07340"
     CDS             6756..7754
                     /locus_tag="VMZ53_07340"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HUS28304.1"
                     /translation="MQSRKIGVLLGGLSSEREVSLRTGEAVLNALRERGHDVIPIYVD
                     HDVDVALRQEQIDVAFIALHGRWGEDGYIQGLLELLGIPYTGSDVMSSALAMHKGKAK
                     ELFRLHNLPTPAYYTLTEEDCADLPGVHGDFGFPCVVKPIREGSSVGVAICHSLEEMG
                     PAVEKALCFDDEVLVERFISGKEVSVAVLGDRALGACEIAPRAGWYDYQNKYTKGSTD
                     YFVPPRLSPERYRGVLAQALRAHLALGCQGATRVDMMVSESGNEFILEVNTVPGLTPT
                     SLLPKIADAAGISFGELCEMMLASASLSSRRGRGERRVIQRTFVGEDRREAMPPTH"
     gene            7755..9770
                     /locus_tag="VMZ53_07345"
     CDS             7755..9770
                     /locus_tag="VMZ53_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HUS28305.1"
                     /translation="MKLRREHRIVAITAVFAVGLPVAASCFIGSRTDALADRIGKAGN
                     VRATIGRVDADLTGTVRLSDVSLGDLFSAESLEASVALETLLAGQFSADEIRVAAPHI
                     SISVDKDGDSDLARLIRRLANAHPDEPAGPALGRAPAPSPKKPARIRRIVVSSGTLVA
                     KIAGVGEVAADDVELIPDAGGVRVITGQVRLRGGTDAVHGELVMARSAAELSLPHVKF
                     RRVLGVAGKGMLVIGDRVVALRDVALGRLSPSSSLEAHGYLDDGAVPRAISVELLHHD
                     GFSLALRGDRVPLSALAPLAPPAIGLADAHATGSLQVRKAHDQIQLVVDGSVTGLRLD
                     HKAIAPGPIPVDATVAASLTVSPQAIAVERAQLGFGAAQWTGSGWLRRGTPMSGQLDL
                     QLAPATCMDLIRSIPLEVRGPIDGIAMTGTFGGHARLSIDLAAPQGEGVELETSLANN
                     CDVQAEPPAADVSTLRIDPEMPDDWVLLHRLPSYVPNAFVSAEDGRFWEHSGFDPIQI
                     ARSLEIDLRDRKIARGGSTISQQLIKNTFLTMRRSFDRKVQEAVLTWRLESRLDKKTI
                     LERYLNIIELGPRTFGIRAASVYWFGKSPRELTVKQAAFLAALTSEPTSMGRRVRRAG
                     GLDPQSADRVDIILRAMTRDGVINKDDLAAARADSMHFSATALRQET"
     gene            9869..11170
                     /locus_tag="VMZ53_07350"
     CDS             9869..11170
                     /locus_tag="VMZ53_07350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS28306.1"
                     /translation="MEHRKVHPWVWLFLNLPFGATSGFVSVMLGFLLKKQGMGDDVIA
                     SLVAINLLPHTWKVFWAPIADSTLTRKRWYVLGNVASCATILGLAFVPVTKANIGVLE
                     WMVFVNSVAITLVGMSVEGLMAHATPPEERGRAAGWFQAGNLGGAGLGGGLGLYLAER
                     ISSQFAFIVIAAALGACTFALQLVPEAPRLLEEGSDAVRTVGEKAKKLFARVIEVFRE
                     LFQMVLSRRGVVGLTLCFLPIGSAAASGIFSGDTAKEWGANANLVAITTGVAAGVVGA
                     VGCFIGGLMSDRLGRRMAYALSGVIMAVVAVGMAVAPQNPTMYAVFSLLYQLGAGIAY
                     GTFTGFVLEIIGKGAAATKYNLLASLSNIPIAYMTKVDGWASVKYGPVNMLFVDAGSE
                     IAGIVVFVLVLLIVRPDKERLPKEEKPSVPEARVVNQSSDR"
     gene            complement(11190..11921)
                     /locus_tag="VMZ53_07355"
     CDS             complement(11190..11921)
                     /locus_tag="VMZ53_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUS28307.1"
                     /translation="MRIGIFSDVHANIEAMSAVLEAFKAERIDKYVCIGDVVGYGASP
                     NECCDLIRKTAAFTILGNHDAAVAGRMDYSYYYDAARQALDLHARQLTPENMEWLKAL
                     PYEVREGDITFCHGSPINLEEFEYIFSVEQATRCLDIWDDLGMVTFIGHSHLCKSFAL
                     TREEVFEVVATKFAIRPEHRYIISVGSVGQPRDYDNRASYTIYDTSEKTFEFKRVAYD
                     IDGAAQKIFAADLERNFGNRLFLGV"
     gene            complement(11966..14830)
                     /locus_tag="VMZ53_07360"
     CDS             complement(11966..14830)
                     /locus_tag="VMZ53_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28308.1"
                     /translation="MARETSARARSQNAYKGVIGQTQTQTLHVTTARGDLIASKVYTS
                     IDALSDPELVERLYADTLNTLRLDGADAVSLSVPVVYHDPAAEVMVLVLGHAQRHREL
                     EERIRVLEQLLADPSPVPAYAKDFGVVFDGAGLRKYLEGRVQQVLAARDSSKDVKDVE
                     RKQKDLATREAELQRALQELVKGEQELARAQKELARTQKEIESARSQHEIAMAEQHRE
                     KLEIERSKSELEKMRSEARARVIAAVQAPADAAPGHAREHDDIVTKPIGRQEIEDIET
                     SVVSAIAKLTAPPPKNEFESEPATRFDARAHVPTNGARPHTASTFTEDEKTGDDGKVP
                     AVLGDVEVDDEPTGNSGVPAGTDPLTTEAQELPGEPAVKAPATSTFSLEGGKVRLALV
                     AGEQLASHLAGPLDIRVLLHRMTTYPIITVLLGPPVAMRTPKPTQLAVVPMDIAGETD
                     RAVLACLGKKFELTVDVFTGGKLLRRVKLAAPLAENVAYIVRAADDHLRGITADGETE
                     PSYARARDLALGAGYDLLGVEHAEASEFRDDKLALLSTAQHLRRALSIARRFARPSRE
                     DYLVCARGYPLVRWRELRRGVLEAAVRWGLWMGPELAQIAVSEGLARSRRDLIMKLDN
                     GFETLRKDAGAFDIDADAADDNAKEIAEEARALGVELRRKSNGAIKSEEHSMVSGSID
                     GTPPSGVSKTRSTDELITLLDDKANRVAAATELCDRADARAAGPVIAAAKKMSRAEAV
                     RILGKSVKFGTAAANPLIEGLSSSKAFLRHGCALALALLRTDEGTHAVIDLLLTEPTE
                     IWREIARAIGQIGPTALMPLASHVGRLGDVTPTTAERVAWAMAHVGVRGGKAALDQMA
                     AGQSIMAPIAKKALELHANAARDEVRVRPGEPGRDVTVNRAFSRRFFEALEADRPDVA
                     QAALHDLDASSPLELDDSDLLVEDGEDDEAELDESDLIQT"
     gene            complement(15054..15695)
                     /locus_tag="VMZ53_07365"
     CDS             complement(15054..15695)
                     /locus_tag="VMZ53_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28309.1"
                     /translation="MDDPVPARVREYADQAVAYVKRTLGVTLEYDSDTLPLLDHYLRS
                     LPEAGINPATIAAAEAAGEPAKKIDDSKESSAILQLVIATSGAYFGEVVRRRLGGRWE
                     AGAEEGTWRVVLPTGINFSPVGIVAAAIAQADLDDVDSEIDAPPRMRPYLQQVLARMG
                     EVSVEDYYSLCGRLDTLEHVHEVLVAVAAQMMGNTDDDDVPEPLPDEPSGPVN"
     gene            complement(15753..16682)
                     /locus_tag="VMZ53_07370"
     CDS             complement(15753..16682)
                     /locus_tag="VMZ53_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HUS28310.1"
                     /translation="MSRVLAIAVLLAAVVPAAADPKAPVVEKSRVEVKPAGKPFKQLQ
                     IDNPLGNVKIEGHDGSGIMIETHKSAPDDETLDRLRVSLVPDPDGTVRIVTAADPSPE
                     AHPQPRSAVSVDIIIHAPRDARIDASVGSGKLEVINMDGGGELDSASGAISVKNVSGE
                     LYTHSLSGRMTLTQVFGPLDAATVSSDLDLDTIGGDRLVASANQGKIAGRRVRARDVE
                     LTTMSGNISLEGESTLRGHIVVSSLHGDVDVRLRRHGALLVKARGTKVDFGSAKSQLL
                     SNGWAQSTIGQGQDPAIVELHSQHGIVRFAIVQ"
     gene            complement(16679..17422)
                     /locus_tag="VMZ53_07375"
     CDS             complement(16679..17422)
                     /locus_tag="VMZ53_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HUS28311.1"
                     /translation="MSCESIRKQLTAYLDGELEGERGSIVRGHLRGCDECRKIASDES
                     LLRDGLRALPPMDPPASMWAGVQAKLAAAEVAHAAQPAWRRFFARLAPQAPQLGFASL
                     AVAAAIIFFVVRARHEEAPAVAETTNIPPVVIAPDHQTPPTPPPIACGTASQASDVTD
                     QIAAEPSQLTACYAQAAHELRELLPEARAKWTDDHKHEVDAKLLAFEKEVADAKDERA
                     RQKSYRAQIRYLQRAVIRDDVALASGGTP"
     gene            complement(17419..17964)
                     /locus_tag="VMZ53_07380"
     CDS             complement(17419..17964)
                     /locus_tag="VMZ53_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS28312.1"
                     /translation="MSSTLTDDAQLIAACRRGEARAMEMLYHQFKRRVFGMAHRIVGP
                     NDAEEVAQEVFVRVYRALANFRGDSALSTWIYRLTVNASLSHLARRGRRKEVPEENLP
                     EVAATPEPERDPGLAARIEAALQELPAGYRAILVLHDVEGLSHEECAAILECRVGTCK
                     SQLHKARARMRELLGNIGGRS"
     gene            18179..19072
                     /locus_tag="VMZ53_07385"
     CDS             18179..19072
                     /locus_tag="VMZ53_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HUS28313.1"
                     /translation="MQSPLVVGLGRVGVAISGLAPNLPADAAPATQLAALATSPAPGV
                     GVALKAVVRLGCRARAVGTHGADLLGHLARASLREAGIDTELLRPSGRSPCDLVTVAG
                     DGTLRTHYAGETGEPVQSDASSAIKDAAAVLIDGTLPVEELRAAELARAQKLPVILDI
                     GELRDGSSELIASADILIISERAASELAPRGELTDALGEILALGPRAVVITLGDKGAI
                     GRHGEQIVKCPAFPSDVLDSFGAGSVFHGAFAAALLSELPFARCIELAAAAASLSLRE
                     LGPWQAMPSRDEVLALVRTRR"
     gene            19083..19715
                     /locus_tag="VMZ53_07390"
     CDS             19083..19715
                     /locus_tag="VMZ53_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28314.1"
                     /translation="MRAVARWVVGMSVVLASAQAFGEAAPAPPPPQQAAPQQQPAPTP
                     VKAAPKKAAPAAKQAPKQVPPAGDGAQAASPPGPRCEIVPTKGTPIFESRYLAGKKAV
                     ITKLYASGTYTRVVAKQSPLRTACIEKDRLAAIRTALAESRWQTTKSPATCQAVSAEA
                     TEIYAAGKLRFTSRTCNPLVLDEKSSYALDLVGMYVGPFGLDIAAELQLQ"
     gene            19712..20179
                     /locus_tag="VMZ53_07395"
     CDS             19712..20179
                     /locus_tag="VMZ53_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28315.1"
                     /translation="MIRAALALLVLAGSANASPVTKCKPGKGTPIFEARWRSGGARVV
                     TKLYASGMWTRLATSPPQRSEASSGCIEPPTIDEIKAALKTVKWKKPKPQEKCYGVDV
                     TPTDFFAGGQKRYTRQSCGGYELDEASAKLIDVIEHQIPMRFENPALPDYEEI"
     gene            complement(20176..20889)
                     /gene="rnc"
                     /locus_tag="VMZ53_07400"
     CDS             complement(20176..20889)
                     /gene="rnc"
                     /locus_tag="VMZ53_07400"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HUS28316.1"
                     /translation="MADYSELEAVLGYSFKERELLEQALRHPSWCNEQGDPRPEDNER
                     FEFLGDAVLDLVVSHRVMTRFPNAHEGDLSVTRQQVVSEAALSEVARQLEIGTWILLG
                     KGEEKSGGRDKPKILADMFEALIGAVYLDGGYASAWDMIDRVLGAKIASVELKNFYDF
                     KTRLQETAQARLKATPTYHVVQELGPDHDKRFVVAVTIGTDEWARAVGKSKKEAEQMA
                     AADAHFRLEGSDVGRPPKR"
     gene            20918..>25374
                     /locus_tag="VMZ53_07405"
     CDS             20918..>25374
                     /locus_tag="VMZ53_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28317.1"
                     /translation="MEALRERFGKYHVLEKIAQGGMAEVYKVKTIGIAGFEKIQALKR
                     ILPQQAREGRFIRSFIDEARIAVELTHRNIVQVFDFGKADGELFMAMELIEGRDLRTG
                     MLQAVSRDCACPIGVAAYVISEVASGLDYAHRKTDGYGGGLGIVHCDVSPSNVMLSAD
                     GYVKILDFGIARATFSSALERRRLRGKPRYMAPEQTLGEPPTAAADVFALGIIAWELF
                     TGLPLYRGHDIKSILEAVRKTDPARIDRMNPKVPKEIVDAVATALTRDPAGRGTAADV
                     AAACTRAAMTSGARQLAAWLRDLDERAADSASISWNALPPSASVPKKSTRSGTASSGP
                     PLRESTRSMTGSAVAVPAAQEFGEGTRSIDAPLPPPTAPPVPLTVIAPVLARPRATSA
                     GGPVPPPLPPAERTATAILTEAHRAASEPPTDVNAFGDRGATSAGGFADRGTTAVGGG
                     FADRGTTAVGGPTFMERATTTGSQYGAFGDRDPTSSVTVGRPFAHEGVTPVVGAPKFD
                     VSTTTSATRPAGRWDHVENTAAGIGKLQDAFDAYDAPRQVPTDTTVAPLPPRKYDRFQ
                     PLTGTNPNALSFDDDDATQFGFAAPDAASFNDLQVELDDEPVAPEPGLHDEPIDDDLG
                     ALATAAPTERRRTVVVAAMLDGAPADILRPIAKSLGGLAYQRGGVVLQMTDEALLVAF
                     GLEVAGEDDVAIAMGWALDAAALTRDVSGTDHGTAGPTLRIGARTGVATASVPRAGST
                     DEEATTVGRVPPDAIDEAWALAREAHPDKPLFVGAAGRVTSGLYELREVPAPKRIARR
                     SRVIEVVAPRGFGDRDRARLERRGNFIGRGAQLAELQTWFDRAIAADRRLTALVAGPA
                     GIGKSRLVAELVAKRQADNTPMRVVMTAANPASRYAPFALVIDLYQAALGLAPARGRV
                     ARQQVVHRLLHTLKEAGMADERSRVVATDLDRAMELRDGVALSSELADLRPRISAGLA
                     AFRTAMIDRQRPMLTIIEDIHLSDSASIEVLRHTLAVTALGPELLVLTGRPDVQIPPP
                     VDVVIEIGDLVGPDLRALVTDRLGDAATPLNIAAVLARGGGNPLFVEELAQAVREQGE
                     DVPATVRDVIAARIDRLSTKAKIALRFGAVLGSTVRARLLEELMDEENLEAQFDELVD
                     GGFLVRPAAAAHSNEGELAFARGLVREVIYDALSRNVQREYHARIGRLLASRFFAGRE
                     EPPAVIADHMERGGEIAGAAAFWLRAGKLALAASDAVAAVTHFSRTLELEGELGAVPP
                     TLTSRARRREALAGREEANRMLGDVVSYPYDLDALEQLCDGDTRRLADVSIRRAHRLL
                     RLADYAGANDATVLAEDYAMAVGDSRLRGEALRVRSEILERLGRFDEALIVVDDAREL
                     FSREGAVADEMAAMVGRGRIHLMRAHYESARDAYRPVISRIEKTGDPWLERIVQNHVA
                     IIEMCLGNFTLAMTSAQRSLELCRRYGDRSREGDALSVAGIILLEVGLY"
BASE COUNT         3976 a         8520 c         8540 g         4338 t
ORIGIN      
        1 tctttcgtgc ggcgctcgag agcgatgcgg acttcgaaca gcttccgggt gagcgccctg
       61 cgcagctgcg atcgcgcgac gatggtgcgg cgctcttgcg gcgtttgttg acgctgtcgc
      121 ggcggctgaa cagtgaagag agcgtcgagc gaatcctcga cgaggtgatc gatacggcga
      181 tcgagctgac ggcggcggag cgcggcttct tgttgttgcg gcaggcggat ggcgagctgg
      241 cgcccgtcgt gtcgcgcaat tttgcgatgg gtgatttgga gagcggcgag cgatcggtga
      301 gtcgatcgat tgccgagcgc gcggcgcaga cgggcgagcc ggtggtgacc gtcgatgcgg
      361 gcgtcgacga gcgattcgcg gtcgcggcgt cggtggcggc gctgcggttg cgatctgtgt
      421 tggtcgtgcc gctgcggcag agggcagcga tcacgggttg catctacgtc gatcaccgtc
      481 tgcgcggtgg ggcgttcgac gactcggccg ccagcttgct cggtgagctc gccgatattg
      541 cggccatcgc gatcgagaac gcgcgcctga ccgacgatct gcgcaagacg agccgcgagg
      601 tcgagacgtt gaaccggcgc ctcgctgcgg agctcgcgga tcgcgatgcc gagctcgtac
      661 gcgtgaaggc ggacttgcca tcccgcgatc gtctgcgcaa cgcgtacgag ggcatcgtcg
      721 gcaagtcgcc tgcgatggtg cgcttgctcg acaccgtcga tcgcgcggcc gcgacgtcac
      781 tgcctgtcgt gatcgttggc gagagcggaa ccggcaagga gctcatcgcg cgcgcgttgc
      841 acgatcacgg gccgcgacgc gacggcgcgt ttgtcgcgat caactgcagc gccgttccgg
      901 agccgctgct cgagagcgag ctattcggac acgtgcgcgg cgcattcacc ggcgcggaac
      961 gcgatcggcg tggcctgttc gaggtcgccg acggcggcac gctgttcctc gacgagatcg
     1021 ccgacacgag ccctggcatg caagcgaagc tcttgcgcgt gttgcaggac ggcatgatcc
     1081 gccgcgtggg cgatagcaag acgcgcaaag tcgacgttcg cgtcgtcgca gcgagccagc
     1141 gttcactcgc cgagctcgca gcggccggta cgttccgcga agacctgcgg tttcgcctcg
     1201 aagtgatcac cgtgcccgtg ccgccgctgc gcgaacgcga tggcgacgtg ccgctcctcg
     1261 tcgagaaatt gctcgccctg ctctgccgcg gtcgcacacc gcccaagatc acgcgcgccg
     1321 cgatgcgagc gctcggccag catcggtggc cgggtaacgt gcgcgagcta gagaacgccc
     1381 tcgcgcgtgg tgtcgcgctc ggcggcgatg tgatcgacgt tgcggatctg cccgaggccg
     1441 ttgccgcgtc ggcgtcgcga cccgagcccg cgaagcctgc cgtcggcgaa gatctgcggc
     1501 tcaagccagc gctcgtcgca acggagcagt cgtacatcgg cgccgcgatg acgcgcgcga
     1561 aaggcaatca gacggtcgcg gcgcgcttgc tcgggctgtc gcggttcggc ctgcagaaga
     1621 agctgcgacg tctagcgggc gaagacgacg gatcttccga cgacgaatag ccacgcacac
     1681 gagagtcttc cgacgatgaa tagctacgga aacggcgacc gatacaggcc gtctcctgcg
     1741 atcgcgacca gcaggtagcc atcgacatcg cgcgcgagca gcatcggcgt cttcccgacg
     1801 aggcccgcga gtcgcgtcca ggtgacgccg gaatcgtgcg acacgtagac gcccggactc
     1861 gtagctgcga tccacgtcgt gtcctcgatc cagaggtcgc gcacgtggat acccgcgagc
     1921 ccgtgcggtt gccacgttcc ttcgagagac cacaacccgt cttcggagcc agcgagcacc
     1981 aagccgcctg gcccagcgaa cggcttttcc agtccgctca ttgcgggtag tggctgaaac
     2041 gaagcaccct gattcgtcga gcgaaacaag ccctcatcac acgcgattac aacgtcgtcg
     2101 ttcgtcagga ccgtcgcttg ataacaattc tgtgggcccg ttccgaccag caccgccgtc
     2161 cacgccgtcc atggcggatc cgcgtagagc gcctgcgtgc gcgtcacaac gatcaagcgt
     2221 ccggacgaga gcgaggcgac gtcgtacgcg ccgaagccat tcgctgcacc ggtgtcgaag
     2281 actgctgcaa acgtgttgcc gcgattgttg ctcacgctga cgtgccgccc cgtgacgacc
     2341 accgatgcct cgtgctggcc cggcacgaca atgccgttga tgtccgatgc gaacccgccc
     2401 agctcccaca cgaggttgta tgtcttgccg ccatcgttcg atacgagcgc acccgttggc
     2461 gtcgcccagt acacaccatc gcgggaaggc gttagtgcga cgcgatcgag cgtccaacca
     2521 gcgatcccgg tgtggcgcgc ttcgaacggt ccggtgccct gtcgaaagaa gacgcctcga
     2581 ctcgtcgcga tgtactggcc tcccatgccg tcgaacacgg cgtcgtgcgg gaccgagccc
     2641 tgcatgaccg atgagcggat gtcgacagtc cccgtgccat ctgtcgactc gaacagcgag
     2701 ttgtaattcg cgaacaggaa gtggttcgga tcgttcggat cgaccgcaac gttgaaccca
     2761 ccgttggcgt agatgttcgt ccacgtgacg ccaccgtcaa ccgacgagaa caacgcgccg
     2821 gtcgtgccac ctgcggtcac ctcgacggcg tacgcgcgca tcgggttcga gggcgcgatc
     2881 ctgatggcgc cgacgtcgag gttttgtagg cccgtgttgc tcacgctcca cgactggccg
     2941 ttgtcgatcg agcgcgcgat gccgccgacc tgcgatccga tcaacatcag cgatggctgc
     3001 agcgacaacc ccgtgatcgc ttggccttcg aatggcgtcg aaatcgtgcg ccactgagcg
     3061 ccgtcccatg cgcgcaagcc gtcatcggtg cccgcgtaca gcgtcgagcc gagcaccgcg
     3121 agctcgtgcg tgtagatgtg gagctccgtc gagcgccagt gcgcacatcc gtcgtcgctg
     3181 acgaacacgt cgcccggatc atcggacgcg aagatcgtgt cgcttcccat gatcgcaacg
     3241 tggcgcgtgc ggagctcgcc gcacggtgtc cacgtgacgc cgcgatcacg cgaccgatac
     3301 atgccgtggt acggcaacgc cacgaagacg tcgccgtttg ctgcgagcgc gacgttgttc
     3361 acgtcgccac cgggcgggag cgctaccggt gtgaataacc ccagttcctg cgcgtcggcc
     3421 ggtgcggcat cgtcgcgtgc atcgaatgcg atgcgcccgc agcccgcaag gaggagcagc
     3481 agcgcgacgc aaaacctcac ctcaagagtg tagacggatt cgaaccaagg cgagctgagc
     3541 gatgcgatcc accatctgcg gataggtcat ccccgcagcc gccgccgcac gcgcgacgcc
     3601 tgcgtcgggt gagatatcgg gattcgggtt gacgtcgatc acccatggca cgcccgcatc
     3661 gtcgacgcgc agatcgacgc ggccgtagtc gcgcagctcg accgctgccc acgcggcacg
     3721 cgacacgcgc tcgatctctg cacgcaccgc atctgacaag ccgcgcagcg gaacgggctt
     3781 cgtgcccgcg taatcgacgt gcttctcgtc ccacttcgcc gcgtaactga cgatcttcgg
     3841 gcgatccgac ggcatcgccg cgaaatcgat ctcgtgcagc ggcagcacct cgacgtgctc
     3901 gccggagcca aacagcgtga cgttgacctc gcggccttcg acgtagcgct ctgcgatcac
     3961 gccctcgtgg aattcggcca tcagctcgcg gcttcgtgcg cgcagatcgg cggcagagcg
     4021 caccacgttt gcctcggtga tgccgagcga tgcgtcctcg tgcgcgagct tcacgaacca
     4081 cgggtagtcg agcttctcga gcgatgcgtc atcgagatcc gcgggcgtgc gcagataccg
     4141 atacggcggc gtcggtacgc cttgcgcgag caggatgtcc ttcgtgcgct gcttgtacag
     4201 gcacgacgcg agcgcgagca gatcggcgcc cgtgtatgga atgtgaaaga gatcgaggag
     4261 acctgcgaac gtcggctcgt tgcgcgggtc gccgtccatc gattcgcaga ggttgaacag
     4321 caggtcgggc ttgtcgcggc cgatccgcgc gagcacgtcg tagacctcga tgccgtgcac
     4381 gcccgtcagc tcgacggtgt gccctgtctc catcagagcg cgcgcgatcg cttttgctga
     4441 ttcccgcacg ctcgtcgcgt cggggttgcc cgcattcgcc gcctgctctt cgtcgtagtc
     4501 ggtattgtag aggacgacga tccgcgccat gcgctcttat aacagcgccg cgaccagccg
     4561 aggtcccgct tcgcgcgcga cttgctcgac gctgactgct ggcaatccgc gcgcttcgag
     4621 ctcgcgaacg acggatgttt gcgggagcga aagcccgcac gggcggattt ttctccacgc
     4681 gtcgggaggc gttgcgacgt cgagcgcgaa gccatgcacg ctcacgccgc gcgcgatgtg
     4741 gatgccgcag gcgccgactt tgttgtcgcc gtaatagatg ccggcaggat cgcggtggaa
     4801 ctctgcacca ggtgcgccga gcgccgcgca agcatctgcg agggccgcgg caacccgcga
     4861 cagaaactcg acgacgccat gctgcacgcg cacgaccgga tagaccatca gttggccagg
     4921 gccgtgatac gtgacgtcgc ccccgcgttc gatgcgctcg atctgtacgc cgtctgccgc
     4981 gaggatgttt gcggtattcg cgctgcgacc gagcgtgatg accggaggat gctcgcacag
     5041 gaagatcgtt cccggcgcgc ctgcccacac gcgctcgcgt tcggcgagct ggtcgtcgag
     5101 cacggggcga tagtccgcgc ggccaagcca acgccactcg agggcggcgg ccgaaggcgc
     5161 ggcggcgagc atcagcccgc gagcagctct tcgaggagct gatcgtcgag gtagtgcagc
     5221 ggtgcagcgg cgctgacgac accgacgccc gcttggatca gcgcagcgac aagctggtcg
     5281 ccgcagacga cgctgatcga acgaccggca cgctcgagct cgcgctcggc atcttcggag
     5341 agctctcccg gcgtgatgag gatgcccgtg acgagatcct tcgcttcgac gccgacgcgc
     5401 agctcaccga cgccgcggcg atcgatcggc tcggagcccg accgcgccga gatgagcacg
     5461 gtgcgatcga ggcccggcgc gatcgctgtc gcgtagctga tgccaccgat gcgcttcacc
     5521 cagtcgatat cgcgataacc ggccgcgacg agatacgcat gaatgatgcg ctcgaagcct
     5581 gcgggcgacg ccttcgcgag acgctgcgag agtgcgcgat gtgtcgcgct tgcgagcttc
     5641 gatagcgcgc tcgcgagccc ggcctcggcg tccgccgtcg ccgacatcga gacgggcccc
     5701 ggcgagaaca gatcgcgacc gcgatagacg atgcgcggac gcagaccgag cgtgcggtag
     5761 ctgcgctcgt cgccgaggag ctcggccttg agctgcggcc acaactgctc tggatcctga
     5821 ttcacgagcg accgcttgcg catcatcgcg gcgagctggc gaaccggcag tggctggcca
     5881 ttgcgcagct gctggaacac cgccgacgct gcatcgccga gcggcgtacc gacgcgcgcg
     5941 agctgatcgt gctcgtggcc attgatctgg ggcgcgctcg ggcgtggcgg ttgcggcgct
     6001 gcctgctgga actgctgcgg cggacgcggc tcgccttgcg ggcgcggctc gcggttgtca
     6061 cggtgatcgc ggctctgtcc gtcgcggctc tgaccgtcgc ggccctgacc gtcgcgctgt
     6121 tcctgcggcg ggcgctgtcc cggatggcca ccctcgcgcc gagcacggcg ctcgtcacgc
     6181 tcgcggcgac gacgctcgcg gcgatcttcc cagcgcgcct tgcgctcgtc gcgcgcgttg
     6241 atctccggca tcatcggccg gtcttcgtcg cgggtcttgt tgtcctggaa ctcgcggtgc
     6301 gcggtggcgg gcttcgtcag gtcgtcgccg tagatcgcgg agagctcctg ctcctcctcg
     6361 gagagcgcgg ttggcggctc gaccgcatcg ttctcgtcgg tcgaatcggc catggccgac
     6421 gctggcgtct cggcgatgat ctcggcctcg atcacatcgg cgacttcggg cgcggtgtcg
     6481 acgacggcat tctcggactc gtcggcgtcc ttcttcttgc tgcgcgactt cttgggcgca
     6541 gccgccttct tcgcggccgg tttcttggcc gcggtcttct tcttcgtctt cgtcgcttcg
     6601 gcgtcgccct cggcgtcccc cgagctcttc ttctttgcca tgcgggggcc ttcctaccac
     6661 gtttggcgcc gaaaaattgc ccctcgaaac accggccttt acgttcggat tacggcggga
     6721 tgcgcaggcg ttctgtcgat caaaggagtc tggatatgca gtcccgcaag atcggtgtgt
     6781 tgctcggtgg tctgtcgagc gagcgcgagg tgagcctccg aacaggtgag gccgtcctga
     6841 acgcgctacg cgagcgcggc cacgatgtca tcccgatcta cgtcgaccat gacgtcgatg
     6901 tggcgctgcg gcaggagcag atcgacgtcg cgttcatcgc gctgcacggc cgctggggcg
     6961 aggacggcta catccagggc ctgctcgagc tgctcggcat cccgtacacg ggatcggacg
     7021 tcatgtcgtc cgcgctcgcg atgcacaagg gtaaggcgaa ggagctgttc cgcctgcata
     7081 acctgccgac accggcctac tacacgctga ccgaagaaga ctgcgccgac ctcccgggcg
     7141 tgcacggcga cttcggcttt ccgtgcgtcg tgaagccgat ccgcgaaggc agctcggtgg
     7201 gcgtcgcgat ctgtcactcg ctcgaggaga tggggccggc cgtcgagaag gcgctgtgct
     7261 tcgacgacga ggtgcttgtg gagcgtttca tcagcggcaa agaggtctcg gtcgccgtgc
     7321 tcggtgaccg cgcgctcggc gcctgcgaga tcgctccgcg cgccggctgg tacgactacc
     7381 agaacaagta cacgaagggc tcgacggact acttcgtgcc gcctcgcctc tcgcccgagc
     7441 gttaccgcgg cgtgctcgcc caggcgctgc gcgcacacct cgcgctcggc tgccagggtg
     7501 cgacccgcgt cgacatgatg gtgagcgaga gcggcaacga attcatcctc gaggtcaaca
     7561 cggttcccgg cctcacgccg acgagcctgc ttccgaagat cgccgacgcc gctggcatct
     7621 cgtttggcga gctgtgcgag atgatgctcg cgagcgcatc gctgtcgtcg cgccgcggtc
     7681 gtggcgagcg ccgcgtcatt caacgcacgt tcgtcggcga ggaccgccgc gaagccatgc
     7741 ccccgacgca ctgaatgaag ctgcgcagag agcaccggat cgtcgccatc acggccgtgt
     7801 tcgcggtcgg gcttccggtc gcggcgtcgt gcttcatcgg ttcgcgcacg gatgccctcg
     7861 ccgatcgcat cggcaaagcg ggcaacgtgc gcgcgacgat cggccgcgtc gatgccgacc
     7921 tcaccgggac ggtccggctt tcggacgtct cgctcggcga cctcttctcc gccgaatcgc
     7981 tcgaggcgtc ggtggcgctc gagacgctgc tcgccggcca gttctccgcg gacgagatcc
     8041 gcgtcgccgc cccacatatc tcgatctccg tcgacaaaga cggcgactcc gatctcgcgc
     8101 gcctgatccg ccgccttgcg aacgcgcatc ccgacgagcc agcaggcccc gcgctcggac
     8161 gagcccctgc cccatcgccg aagaagcccg cgcgcatccg ccgcatcgtc gtcagctcgg
     8221 ggacgctcgt cgcaaagatc gcaggtgtcg gcgaggtcgc tgcagacgat gtcgagctga
     8281 ttccggacgc cggcggcgtt cgcgtgatca ccggccaagt tcgcctgcgt ggcggcaccg
     8341 acgcggttca cggggagctc gtcatggcgc gcagtgccgc cgagctctcg ctgccgcacg
     8401 tcaaattccg ccgcgtcctc ggcgtcgcgg gcaagggcat gctcgtgatc ggcgatcggg
     8461 tcgtcgcgct gcgcgatgtc gcgctcggtc gcctctcccc gtcgagtagc ctcgaagcgc
     8521 acggctatct cgacgatggc gctgtcccgc gcgcgatctc cgtcgagctt ctccatcacg
     8581 acggcttctc actcgcgctt cgcggcgatc gcgtcccact ttccgcgctc gcgccgctcg
     8641 ctccacccgc gatcggcctc gccgacgcac acgccaccgg ctcgctccaa gtccgcaaag
     8701 cgcacgatca gatccagctc gtcgtcgacg gctcggtcac cggccttcgt ctcgatcaca
     8761 aagcgatcgc gcccggcccc atccccgtcg acgccaccgt cgccgcttcg ctcacggtct
     8821 cgccgcaagc gatcgcggtc gagcgcgcgc agctcggctt cggcgcggcc cagtggaccg
     8881 gctcgggctg gctgcgccgc ggcacgccga tgtctggcca gctcgatctc cagctcgcgc
     8941 ctgccacctg catggatctc atccgctcga tcccgctcga agtgcgcggc ccgatcgatg
     9001 gcatcgcgat gacgggcacg ttcggcggcc acgctcgcct gtcgatcgat ctcgcagcgc
     9061 cgcaaggcga aggcgtcgag ctcgagacct cgcttgcgaa caactgcgac gtccaggccg
     9121 agccacctgc cgccgacgtc agcacgctgc gcatcgatcc cgagatgccc gacgactggg
     9181 tcctcctcca ccgcctcccg agctacgtcc ccaacgcatt cgtgtccgcc gaagacggcc
     9241 gattctggga gcacagcggc ttcgatccga tccagatcgc ccgcagcctc gagatcgatc
     9301 tgcgcgaccg caagatcgcg cgcggcggct cgacgatcag ccagcagctc atcaagaaca
     9361 cgttcctcac gatgcgccgc tcgttcgatc gcaaggtcca agaagcggtc ctgacgtggc
     9421 gcctcgaatc gcgcctcgac aagaagacga tcctcgagcg ctatctcaac atcatcgagc
     9481 ttggcccacg cacgttcggc atccgcgccg cctcggtcta ctggttcggc aagtcgccgc
     9541 gcgagctcac cgtcaaacaa gccgcgttcc tcgccgccct gacctcggag ccgacgagca
     9601 tgggacgccg cgtgcgtcgc gccggagggc tcgacccgca gtccgctgat cgcgtcgaca
     9661 tcatcctgcg tgcgatgacg cgcgacggcg tgatcaacaa agacgacctc gccgccgctc
     9721 gcgcggactc gatgcacttc tccgcgacag cgctgcgcca agaaacgtaa tcgcccgttg
     9781 ccgctctcga gcgacgcgtc tctccgaaga cgagggctga tcgttaacgg gctgccaatc
     9841 gacgcggtgt cgtgtaacgt cccgcgccgt ggagcatcgc aaggttcatc cgtgggtctg
     9901 gctctttctg aacctgccgt ttggcgcgac ctctggtttc gtgtccgtga tgctcggctt
     9961 ccttctgaag aagcaaggca tgggcgacga cgtgatcgcg agcctcgtcg cgatcaactt
    10021 gttgccgcac acgtggaagg tcttctgggc cccgatcgcg gacagcacgc tgactcgaaa
    10081 gcgttggtac gtcctgggca acgtcgcgtc atgcgcgacc atcctcgggc tcgcgttcgt
    10141 cccggttacg aaggccaaca ttggcgtcct cgagtggatg gtcttcgtca acagcgtcgc
    10201 catcacgctc gtcggaatgt ccgtcgaggg gctgatggcg cacgcgacac cgccggaaga
    10261 gcgagggcgc gccgccggct ggtttcaggc cggcaacctc ggcggcgccg ggctcggcgg
    10321 cggcctagga ctctatctcg cggagcgcat ctcgtcgcaa ttcgccttca tcgtgattgc
    10381 ggcggcgctc ggagcctgca cgttcgcgct gcagctcgtc cccgaggcgc cacggctcct
    10441 cgaagaaggt tcggatgctg tccggacggt cggcgagaag gcgaagaagc tcttcgctcg
    10501 cgtcatcgag gtgttccgcg agctttttca gatggtgctc agccgtcgtg gcgtcgtcgg
    10561 tctgacgctg tgtttcttgc cgatcggatc ggcggcagcg tcgggcatct tctccggcga
    10621 caccgcgaaa gaatggggcg ccaacgccaa cctcgtcgcg atcacgacgg gcgtcgcagc
    10681 gggcgtcgtc ggcgcggtgg gttgcttcat cggtgggctc atgtcggatc gcctaggtag
    10741 gcgcatggcc tatgcgctct cgggcgtgat catggcagtg gtcgctgtcg gcatggccgt
    10801 cgcaccgcag aacccgacga tgtacgcggt gttctcgctt ctctatcagc taggcgctgg
    10861 tatcgcgtac ggcacgttca ccggcttcgt tctcgagatc atcggcaagg gcgcagcggc
    10921 gacgaagtac aacttgctcg cgtcgctctc gaacatcccg atcgcgtaca tgacgaaggt
    10981 cgacggctgg gcgtcggtga agtacggccc ggtgaacatg ctcttcgtcg atgcgggttc
    11041 ggaaatcgcc ggcatcgtcg tgttcgtgct cgttctcctc atcgtgcgtc ccgacaagga
    11101 acgcttaccg aaggaagaga agccgtccgt gccggaggcc cgggtcgtga accaatcgag
    11161 cgaccgttag acgccgagcc gcgacgtcgt tagacgccga ggaagaggcg gttgccgaag
    11221 ttccgctcga gatccgcagc gaagatcttc tgcgcagcgc catcgatgtc gtacgcgacg
    11281 cgcttgaact cgaaggtctt ctcgctcgtg tcgtagatcg tgtagctcgc gcggttgtcg
    11341 taatcgcgcg gctggccgac cgagccgacc gagatgatgt agcggtgctc ggggcgaatc
    11401 gcgaacttcg tcgccacgac ctcgaacacc tcttcacgcg tcagcgcgaa cgacttgcag
    11461 aggtgcgagt ggccgatgaa cgtgaccatc ccgaggtcgt cccagatatc gaggcagcgc
    11521 gtcgcttgct cgacggagaa gatgtactcg aactcttcga ggttgatcgg cgagccgtgg
    11581 cagaacgtga tgtcgccttc gcggacttcg tacgggagcg ccttgagcca ctccatgttc
    11641 tccggcgtga gctggcgagc gtggaggtcg agcgcctggc gagcggcgtc gtagtagtac
    11701 gagtaatcca tacgacccgc gacggcggcg tcgtggttac cgaggatcgt gaacgctgcg
    11761 gtcttgcgga tcagatcgca acactcgttc ggggacgcgc cgtagccgac gacatcaccg
    11821 atgcagacgt acttgtcgat ccgctccgcc ttgaaagctt cgagcacagc ggacatcgct
    11881 tcgatgttcg cgtgcacgtc ggagaagatt ccgatcctca tgtacggctc gccaatgata
    11941 cccgaagttc ggtcggcacg gcgagctagg tctggatcag atccgattca tcgagctcgg
    12001 cctcgtcatc ctcgccatcc tcgacgagga ggtcggaatc gtcgagctct agagggctgc
    12061 tggcgtcgag gtcgtgaagg gctgcctgtg cgacatctgg ccggtcggcc tccagcgcct
    12121 cgaagaatcg ccgagagaac gcccgattga ccgtcacatc cctgcccggc tcgcccggcc
    12181 taacgcgcac ctcatcccgc gccgcgttgg cgtgcagctc gagcgctttt ttcgcaatcg
    12241 gcgccatgat gctctgaccc gcggccattt gatcgagagc tgccttaccg ccgcggacgc
    12301 ccacatgggc catcgcccag gcgaccctct cggcggtggt cggggtgacg tcgccgaggc
    12361 gtccgacgtg cgacgcgagc ggcatgagcg cggtcggccc gatttggcca atcgcgcgcg
    12421 cgatctcgcg ccagatttcg gtcggctcgg tgagtaacag gtcgatgacg gcgtgggtgc
    12481 cttcatcggt gcgcagcagc gcgagtgcga gcgcacagcc gtggcgcagg aacgccttgc
    12541 tgctcgagag accctcgatg agcgggttgg cggccgcggt tccgaacttc acgctcttgc
    12601 cgagaatgcg cacggcctcc gcgcggctca tcttcttggc agcggcgatc accggcccgg
    12661 cggcgcgagc atccgcgcgg tcgcacagct cggttgcggc ggccacccgg ttcgccttgt
    12721 cgtcgagcag cgtgatcagc tcgtcggtcg agcgcgtctt gctgacgccc gatggcggcg
    12781 tgccgtcgat cgagcccgag accatcgagt gctcttcgct cttgatcgcg ccattggact
    12841 tgcgacgcag ctcgacgccg agcgcgcgcg cttcttcggc gatctctttg gcgttgtcgt
    12901 cggcggcatc ggcgtcgatg tcgaatgcgc cggcatcctt gcgcagcgtc tcgaagccgt
    12961 tgtcgagctt catgatgaga tcgcgacgcg aacgcgcgag gccctcggag accgcgatct
    13021 gcgcgagctc gggacccatc cagaggcccc accgcacggc cgcttcgagc acgccgcggc
    13081 gcagctcgcg ccaacgcacg agcggatagc cacgcgcgca cacgaggtag tcctcgcgcg
    13141 agggacgcgc gaaccggcga gcgatcgaga gtgcgcgacg cagatgctgc gccgtcgaaa
    13201 gcagcgcgag cttgtcatcg cgaaactcgc tcgcttccgc atgctcgacg ccgagcaggt
    13261 cgtagcccgc tcctagcgcg aggtcgcgcg cacgcgcgta ggacggctcc gtctcgccat
    13321 ccgcggtaat gccgcgcagg tgatcgtcgg cggcgcgcac gatgtacgcg acattctcgg
    13381 cgagcggcgc cgcgagcttg acgcgccgaa gcagcttgcc tcccgtgaac acgtcgacgg
    13441 tgagctcgaa cttcttgccg aggcatgcga gcaccgcgcg atcagtctcg ccggcgatgt
    13501 ccatcgggac gacggccagc tgcgttggct tcggcgtgcg catcgcgacc ggcgggccga
    13561 gcagcaccgt gatgatcgga tacgtcgtca tccgatgcag cagcacgcgg atgtcgagcg
    13621 gccccgcgag atgactcgct agctgctcgc ccgcgacgag cgcgagccgg accttgccgc
    13681 cctcgagcga gaacgtcgac gtggcaggtg ccttcaccgc aggctcaccc gggagctcct
    13741 gcgcctcggt tgtcagcgga tcggtgcccg cgggcacgcc cgagttgccg gttggctcat
    13801 catcgacttc aacatcaccg agcacggcgg gaaccttgcc gtcgtcgccg gtcttctcgt
    13861 cctcggtaaa cgtgctcgcg gtatgcgggc gggcgccgtt cgtcgggacg tgcgcgcgtg
    13921 cgtcgaacct cgtcgctggc tcgctctcga attcgttctt cggcggcggc gctgtcagct
    13981 ttgcgatcgc cgagacgacg ctcgtctcga tgtcctcgat ctcctggcga ccgatcggct
    14041 tcgtgacgat gtcgtcgtgc tcccttgcat gaccgggagc agcgtcggca ggcgcctgca
    14101 cggccgcgat cacgcgagct cgcgcctcgg agcgcatctt ttcgagctcc gacttgctgc
    14161 gttcgatctc gagcttctcg cgatgctgct cggccatcgc gatctcgtgc tgcgagcgcg
    14221 ccgactcgat ctccttctgc gtgcgagcga gctccttctg cgctcgcgcg agctcctgct
    14281 cgcccttgac gagctcctgc aacgctcgct gcagctccgc ctcgcgcgtc gcgagatcct
    14341 tctgcttgcg ctcgacgtcc ttgacgtcct tcgacgagtc gcgcgccgcg agcacctgtt
    14401 ggacgcggcc ctcgaggtat ttgcgcagac ctgcgccgtc gaacacaacg ccgaagtctt
    14461 tcgcgtacgc cggcaccggc gacggatccg cgagcagctg ctcgagcacg cgaatgcgct
    14521 cttcgagctc gcgatgccgc tgcgcatgcc cgagcacgag caccatcact tcggcggcgg
    14581 ggtcgtgata gacgacgggc accgagaggc tcactgcgtc cgcgccgtcg aggcgcagcg
    14641 tgttcagcgt atcggcgtag aggcgctcga cgagctccgg atcgctgagc gcatcgatcg
    14701 atgtgtagac cttgctcgcg atcaaatcgc cgcgcgcggt cgtgacgtga agggtctggg
    14761 tctgcgtctg gccgatcacg cctttgtagg cgttttgtga tcgcgcgcga gcagaggtct
    14821 ccctggccat gtcgccaggc ataacacgag cttgggtccg taggcgtatg cgtaggcgta
    14881 agcgggcccg cgcctcgtct tcggctgctc cttctcggcg gttatcggat cggtgcgagg
    14941 catttcgcga aggatgcaac gccacggtgc gttcgacgca cgctgcacga gcgcggagga
    15001 gcgcacggcg tacggcgtgt tctcgcttac gcctacgctt acgcttacgg ttctcagttg
    15061 accgggccag agggctcgtc cggaagcggc tctggcacat cgtcgtcgtc ggtattgccc
    15121 atcatctggg cagctaccgc gaccaggacc tcgtggacgt gctcgagggt gtcgaggcgc
    15181 ccgcacagcg agtagtaatc ctcgaccgag acctcgccca tgcgcgcgag cacctgctgc
    15241 agatacggcc gcattcgcgg cggcgcgtcg atttccgagt cgacatcgtc gagatcggcc
    15301 tgcgcgattg ccgcagcgac gatgccgacc ggcgagaaat tgatgccggt tgggaggacg
    15361 actcgccagg tgccctcttc ggcaccggct tcccagcgac cgccgaggcg ccgccgaacg
    15421 acctcgccga agtacgcgcc cgacgtggcg atcacgagct ggaggatcgc cgaggactcc
    15481 ttggagtcgt cgatcttctt ggctggctca ccagccgcct cggccgccgc gatcgtggcg
    15541 ggattgatgc cggcttcggg cagtgagcgc aggtaatggt cgagcagcgg cagcgtgtcg
    15601 ctgtcgtatt cgagcgtgac cccgagcgtg cgcttgacgt acgcgaccgc ctgatcggcg
    15661 tattcgcgta ctcgcgcggg aaccgggtcg tccatagaag acagcctacc ccgcttcggc
    15721 gcactgcgct attggcggcc agcgactgcg agctactgga cgatcgcaaa tcgcacgatg
    15781 ccgtgctgcg agtgcagctc gacgatcgcc gggtcttggc cctgcccgat cgtcgattgc
    15841 gcccagccgt tcgagagcag ttggctcttt gcagaaccga aatcgacctt cgtgccacgc
    15901 gctttgacga gcagcgcacc atggcggcgg agccgaacat cgacgtcgcc gtgcaggctc
    15961 gacaccacga tgtggccgcg cagcgtgctc tcgccttcga gcgagatgtt gccgctcatc
    16021 gtcgtcagct cgacatcgcg agcgcgcacg cggcgacccg cgatcttgcc ttggttcgcg
    16081 gacgcgacga gccggtcacc gccgatcgta tcgagatcga gatccgacga gacggtcgcc
    16141 gcgtcgaggg ggccaaacac ctgcgtgagc gtcatgcggc ccgagaggct gtgcgtgtag
    16201 agctcgccgg aaacgttctt gacgctgatg gcaccggagg cgctatcgag ctcgccgccg
    16261 ccatccatgt tgatgacctc gagcttgccg ctgccgacgc tcgcatcgat gcgcgcatcg
    16321 cgcggtgcgt ggatgatgat gtcgacggag acggccgagc gcggttgcgg gtgcgcttcc
    16381 ggcgacggat ccgccgcggt cacgatgcga acggtgccgt ccggatcggg aacgagcgac
    16441 acgcgcaggc gatcgagcgt ctcgtcgtcg ggcgcagact tgtgcgtctc gatcatgatg
    16501 ccggagccgt cgtggccctc gatcttcacg ttgccgagag gattgtcgat ctgcagctgc
    16561 ttgaacggct tgccggcggg cttcacctcg acgcgcgatt tctcgacgac gggcgccttc
    16621 ggatcggcgg cggcgggcac gactgccgcg agcagcaccg caatggcgag cacgcgactc
    16681 atggcgtgcc tccactcgcg agcgcgacgt catcgcggat caccgcgcgc tggagatagc
    16741 gaatctgcgc gcgatacgac ttctggcgag cgcgctcgtc cttcgcgtcg gcgacttcct
    16801 tctcgaatgc gaggagcttc gcgtcgacct cgtgcttgtg atcgtccgtc cacttcgcgc
    16861 gcgcttcggg cagcagctcg cggagctcgt gagcagcctg cgcgtaacac gcggtgagct
    16921 ggcttggctc ggcagcgatc tggtcggtca cgtcgcttgc ttggctcgcg gtaccgcatg
    16981 cgatcggagg cggcgttggc ggcgtctgat gatccggcgc gatcacgacg ggcgggatgt
    17041 ttgtcgtctc ggctaccgcg ggcgcttcct cgtgacgcgc acgcacgacg aagaagatga
    17101 tcgcggcagc gaccgccagg ctggcgaagc cgagctgcgg agcttgcggc gcgaggcgtg
    17161 caaagaaccg acgccacgcg ggctgcgctg catgcgcgac ctcggctgca gcaagcttgg
    17221 cctgcacgcc agcccacatg ctcgcgggcg gatccatcgg cggcagtgcg cgaagaccat
    17281 cgcgaaggag cgattcgtcg gacgcgatct tgcggcactc gtcgcagccg cggaggtgcc
    17341 cgcgcacgat gctgccgcgt tcgccttcga gctcaccgtc gagatacgcg gtcagctgtt
    17401 tgcgaatcga ttcgcagctc acgacctgcc cccgatattg ccgagcagct cacgcatgcg
    17461 tgcgcgcgcc ttgtgcagct gtgatttgca cgtgcccacc ctgcactcga ggatcgcagc
    17521 gcattcttcg tgggacaagc cctcgacgtc gtggaggacg aggatcgcgc gatacccggc
    17581 gggcagctct tgcagcgccg cttcgatccg cgccgcgagg cctggatcgc gctcgggctc
    17641 aggagtcgcc gccacttcgg gcaggttctc ctcgggaact tctttgcggc gaccgcggcg
    17701 cgcgagatgc gacagcgacg cgttgacggt aagccgatag atccatgtcg acagcgccga
    17761 gtcgccgcgg aagttcgcga gcgcgcgata cacgcgtacg aagacttctt gcgcgacctc
    17821 ttcggcgtcg tttgggccga cgatgcgatg ggccatgccg aacacccgtc gcttgaactg
    17881 gtggtagagc atctccatcg cgcgcgcttc cccgcgccgg caagcggcga tcagctgcgc
    17941 gtcgtcggtt agcgtggagg acacgcgcgg cttgggttca gcgagtgcgg gcgaaggcag
    18001 ggtttcggcg acggtttcag tcacgggggc cgctgggttg tcagcgctct gaccctccga
    18061 ggacggaagg cgttgcccgg actgctgcga agacacgatg cgacgcactc tacagcccaa
    18121 aacgcgtgtt gtcgcaagag ctttcacgtg cgctcccgca ctgataggat gaggatcgat
    18181 gcagtccccg ctcgtcgttg gcctcggtcg agtcggggtt gcgatctcgg ggctagcgcc
    18241 aaatctgccg gccgatgccg cacctgcgac ccagctcgca gccctggcca cctcgcccgc
    18301 gccgggtgtt ggcgtcgcgc tgaaggcggt cgttcggctc ggctgtcggg cccgcgccgt
    18361 cggcacccac ggcgctgatc tgctcggcca cctcgcgcgc gcgtcgctcc gcgaagccgg
    18421 catcgatacc gagctgctgc ggccgtcggg gcgctcgcct tgcgatctcg tgacggtcgc
    18481 cggtgacggc acgctgcgca cgcactacgc gggcgagacg ggcgagcccg tgcagtcgga
    18541 tgcgagctcg gcgatcaagg atgctgccgc tgtcctcatc gatggcacgc ttcccgtcga
    18601 ggagctgcgc gcggcggagc tggctcgtgc gcagaagctg ccggtcatcc tcgatatcgg
    18661 cgagctgcgg gatggctcga gcgagctgat cgcgtctgcg gacattctga tcatctcgga
    18721 gcgtgcggcc tcggagctcg cgccacgtgg agagctgacg gacgcgctcg gcgaaatcct
    18781 cgcgctcggt ccgcgcgccg tcgtgatcac gctcggcgac aagggcgcga tcggcaggca
    18841 tggcgagcag atcgtgaagt gccccgcgtt tccgtcggac gtcctcgatt cgttcggtgc
    18901 gggctcggtc ttccacggcg cgttcgccgc tgcgctgctc tcggagctgc cgttcgcgcg
    18961 gtgcatcgag cttgccgccg ctgctgcgtc gctgtcgctg agggagctcg ggccatggca
    19021 agcgatgccg tcccgcgacg aagtgctggc actcgttcgg actcggcgct agagatcgag
    19081 ggatgagggc ggtcgctcgc tgggtcgttg gcatgtcggt cgtgctcgcg agcgcgcagg
    19141 cattcggtga agcagcgccc gcgcctcctc ctccgcagca agccgcaccg cagcaacagc
    19201 cggcgccaac accggtgaaa gccgcgccga agaaagcagc acccgcagcc aagcaggcac
    19261 cgaagcaagt gccgcccgct ggtgatggcg cgcaagctgc gtcgccgcct ggcccgcgct
    19321 gcgagatcgt accgacgaag ggcacgccga tcttcgagtc gcgctacctc gccggcaaga
    19381 aggcggtcat cacgaagctc tacgcgtcgg gcacgtacac gcgcgtcgtg gcgaagcagt
    19441 cgccgctgcg cacggcgtgt atcgagaaag atcgcctcgc cgcgatccgc accgcgctcg
    19501 ccgagtcgcg ttggcaaacg acgaagagcc cagcgacatg ccaggccgtc tccgcggagg
    19561 cgaccgagat ctacgcggcg ggcaagctgc ggttcacgtc gcgcacgtgc aacccgctcg
    19621 tcctcgacga gaaaagcagc tacgcgctcg atctcgtcgg catgtacgtc ggtccgttcg
    19681 gcctcgacat cgctgccgag ctgcagctgc aatgatccgc gcggcgctcg ccttgctcgt
    19741 gctcgcaggc agcgcgaatg cgtcgcccgt gacgaagtgc aagccgggca agggcacgcc
    19801 gatcttcgag gcgcgctggc gctcgggcgg cgcgagggtc gtcacgaagc tgtacgcgtc
    19861 gggcatgtgg acgcgactcg cgacatcgcc gccacaacgc agcgaggcca gcagcggctg
    19921 catcgagccg ccgacgatcg acgagatcaa agcggcgctg aagaccgtga agtggaagaa
    19981 gcccaagccg caggagaagt gctacggcgt cgacgtcacg ccgacggact tcttcgccgg
    20041 cggccaaaag cgctacacgc gtcagtcgtg cggcggctac gagctcgacg aagcgtcggc
    20101 gaagctcatc gacgtcatcg agcaccagat cccgatgcgc ttcgagaacc cggcgttgcc
    20161 cgactacgaa gagatctagc gtttcggcgg gcggccgacg tccgagcctt cgaggcggaa
    20221 gtgcgcatcg gcggcggcca tctgttcggc ttcttttttc gatttgccga cggcgcgcgc
    20281 ccactcgtcg gtgccgatcg tgactgcgac gacgaaccgt ttatcgtgat cggggccgag
    20341 ctcttggacg acgtggtacg tcggcgtcgc tttgaggcgc gcttgcgcgg tctcttgcag
    20401 gcgcgtcttg aagtcgtaga agtttttcag ctcgaccgac gcgatcttcg cgccgagcac
    20461 gcgatcgatc atgtcccatg cggaggcata gccaccgtcg aggtagaccg ccccgatgag
    20521 ggcctcgaac atgtcggcga ggatctttgg cttgtcgcgg ccgccggact tttcctcgcc
    20581 tttgccgagc aggatccacg tgccgatttc gagctggcga gcgacctcgg agagcgcggc
    20641 ttcggaaacg acctgctggc gggtgaccga gaggtcgcct tcgtgggcat tggggaagcg
    20701 cgtcatcacc cgatggctga cgaccaggtc gagcacggca tctccgagaa actcgaaccg
    20761 ctcgttgtct tcggggcgag ggtcgccctg ctcgttgcac cacgacgggt ggcgcaaggc
    20821 ttgttctagg agctcgcgct ccttgaatga atagccgagg acggcttcga gctcggagta
    20881 atcggccacg accgccgcca tgataccctc ggaagcggtg gaagcgctgc gcgagcgttt
    20941 cggcaagtac cacgtgctcg agaagatcgc gcagggtggg atggccgagg tctataaggt
    21001 gaagaccatc ggtattgccg ggttcgagaa gatccaggca ctgaagcgca tcctcccgca
    21061 gcaggcgcgc gaaggccggt tcatccgcag cttcatcgac gaggcgcgca tcgcagtgga
    21121 gctcacccac cgcaacatcg tccaggtctt cgacttcggt aaggcggacg gcgagctctt
    21181 catggcgatg gagctcatcg aggggcgcga cctgcgcaca gggatgctgc aggcggtgtc
    21241 gcgcgactgt gcatgcccga tcggtgtggc ggcgtacgtg atctcggagg tcgcgtccgg
    21301 tctcgactat gcccatcgca agaccgacgg ctacggcggt ggcttgggca tcgtccactg
    21361 cgatgtgtcg ccgtcgaacg tgatgctgtc tgccgatggc tacgtgaaga tcctcgattt
    21421 cggcatcgcg cgcgcgacgt tctcgtcggc actcgagcgg cggcgtcttc gcggcaagcc
    21481 gcgctacatg gcgcccgagc agaccctcgg tgagccgccg accgcagcgg cggatgtgtt
    21541 cgcgctcggc atcatcgcgt gggagctgtt caccggattg ccgctctatc gaggtcacga
    21601 catcaagtcg atcttggaag cggtgcgaaa gacggatccc gcgcgcatcg atcggatgaa
    21661 tccgaaagtg ccgaaggaaa tcgtcgacgc agtcgccacg gcgctcacgc gcgatcctgc
    21721 ggggcgcggg acggcggcgg atgtggccgc ggcgtgcacg cgggcggcga tgacctcggg
    21781 cgcgcggcag ctcgctgcgt ggctgcgcga cctcgacgag cgtgcggcgg attcggcgag
    21841 catctcgtgg aacgcgctgc cgccgtcggc gagcgtgccg aagaagtcga cgcgctcggg
    21901 aacggcatcg agcgggccgc cgctgcgcga gtcaacgcga tcgatgacgg gctcggcggt
    21961 cgccgttccg gccgcgcagg agttcggcga gggcacgcga tcgatcgatg cgccgctgcc
    22021 gccaccgacc gccccacccg tgccgctcac cgtcatcgca cctgtgctcg cgcgcccgcg
    22081 agcgacatcc gctggaggtc cggtgccgcc tcccctgccg cccgccgagc ggacggcgac
    22141 ggcgatcctg acagaagcgc accgcgcagc gtcggaacca ccgaccgacg tcaacgcatt
    22201 cggcgatcga ggggcgacgt ctgccggcgg cttcgccgat cgcggcacga ctgctgtcgg
    22261 tggtggcttc gccgatcgcg gcacgaccgc cgtcggtggc ccgaccttca tggagcgcgc
    22321 gacgacgacg ggctcgcagt acggcgcgtt tggcgatcgc gatccgacgt ccagcgtgac
    22381 ggtcggccgc cccttcgcgc acgaaggcgt gacgcccgtc gtcggtgcgc cgaagttcga
    22441 tgtgtcgacg acgacctccg cgacgcgacc cgcggggcgt tgggaccacg tcgagaacac
    22501 cgcggccggc atcggcaagc tgcaagatgc gttcgacgcg tacgatgcgc ctcgccaggt
    22561 gccgacggac acgacggtcg cgccgctgcc accgcgcaaa tacgaccgct tccagccgct
    22621 caccgggaca aatccgaatg cgctgagctt cgacgacgat gatgcgacgc agttcggctt
    22681 cgcggcgccg gatgcggcga gcttcaatga cttgcaggtc gagctcgacg acgagccggt
    22741 cgcgccagag ccaggcctgc acgacgagcc gatcgacgat gacctcggag cgcttgcgac
    22801 tgcggctcca acggagcggc ggcgaacggt cgtcgttgcg gcgatgctcg acggcgcgcc
    22861 tgccgacatt ctgcgtccga tcgcgaagag cctcggcggg ctcgcctacc agcgcggtgg
    22921 tgtcgtgctg cagatgaccg acgaagcgtt gctcgtcgcg ttcggcctcg aggtcgcggg
    22981 tgaagacgac gtcgcgatcg cgatgggctg ggcactcgac gcagccgcgc tcacgcgcga
    23041 tgtcagcggc accgatcacg gcactgcagg cccgacgctg cgcatcggtg cacgcacggg
    23101 cgtcgcgact gcaagcgttc cacgggcagg ctccaccgac gaggaagcga cgaccgtcgg
    23161 ccgcgttccg ccggacgcca tcgatgaagc gtgggcgctc gcgcgtgagg cgcacccaga
    23221 caagccgctg ttcgttggtg cggccggccg cgtgacgagc ggtctctacg agctgcgcga
    23281 ggttcccgcg ccgaagcgga tcgcgagacg cagccgcgtg atcgaggtag tcgcgccacg
    23341 cggctttggc gatcgcgatc gagcgaggct cgaacgtcgc ggcaacttca tcgggcgcgg
    23401 cgcgcagctc gccgagctgc agacctggtt cgatcgcgcg atcgcggccg accgccgact
    23461 caccgcactc gtcgctggac ccgcaggtat cggtaagagc cggctcgtcg cggagctcgt
    23521 cgcgaagcgc caagcggaca acacgccgat gcgcgtcgtc atgaccgccg cgaatccggc
    23581 gagccgctac gcgccgtttg cgctcgtgat cgatctttat caagcagcgc tcggtctcgc
    23641 gcccgcacga ggacgcgtcg cgcggcagca ggtcgtccac cgactgctcc acacgttgaa
    23701 ggaagcgggc atggccgacg agcgctcgcg cgtggtcgcg acggatctcg atcgtgcgat
    23761 ggagctgcgc gatggtgttg cgctgtcgtc ggagcttgcc gacttgcgcc cgcggatctc
    23821 cgctggcctc gcggcattcc gcaccgcgat gatcgatcgc cagcgcccga tgctcacgat
    23881 catcgaggac attcatctct ccgacagcgc gagcatcgag gtgctgcgcc acacgctcgc
    23941 cgtgacggca ctcggtcccg agctgctcgt gctgaccggc aggcccgacg tgcagattcc
    24001 accgcccgtc gatgtcgtga tcgaaatcgg cgacctcgtc gggcctgacc tgcgcgcgct
    24061 cgtcaccgat cggctcggcg atgcagcgac gccgctcaac atcgctgcgg tgctcgcccg
    24121 tggcggcggc aacccgctat tcgtcgaaga gctggcgcaa gccgtgcgcg aacaaggcga
    24181 agacgtgccg gccaccgtcc gcgacgtcat tgcggcacgc atcgaccgcc tctcgacgaa
    24241 ggcgaagatc gcgcttcggt tcggcgcggt gctcggcagc acggttcgcg cgcgcctgct
    24301 cgaggagctg atggacgagg agaacctcga ggcgcagttc gacgagctcg tcgacggcgg
    24361 cttcctcgtg cggccagccg ccgccgcgca ctcgaatgag ggtgagctcg catttgctcg
    24421 cggcctcgtg cgagaggtca tttacgacgc gctgtcgcgc aacgtccaac gcgagtatca
    24481 cgcgcggatc gggcgcctgc tcgcgtcgcg cttcttcgcc ggccgcgaag agccgccggc
    24541 ggtgatcgcc gatcacatgg agcgcggcgg tgaaatcgcg ggcgccgcgg cgttctggct
    24601 gcgcgctggc aagctcgcgc tcgcggcatc cgacgccgtc gcagccgtca cgcacttctc
    24661 gcgtacgctc gagctcgaag gcgagctagg cgcagtcccg ccgacgctga cgtcgcgcgc
    24721 tcgccgccgc gaggctctcg ccgggcgaga agaagcgaat cgcatgctcg gtgatgtcgt
    24781 ctcgtatccg tacgacctcg atgcgctcga gcaactctgc gacggcgaca cacgccgcct
    24841 cgcggatgtc tcgatccgtc gcgcgcatcg cctgctccgc ctcgccgact acgcgggcgc
    24901 gaacgatgcg accgtgctcg cggaagacta cgcgatggct gtcggcgatt cgcgcctgcg
    24961 cggcgaagcg ctccgcgtgc gcagcgagat cctcgagcgc ctcggccgct tcgacgaagc
    25021 cctcatcgtt gtcgatgacg cgcgcgagct gttctcacgc gaaggcgcgg tcgccgatga
    25081 aatggccgcg atggtcggcc gcggccgcat ccacctcatg cgcgcgcact acgaatccgc
    25141 gagagacgca taccgccccg tcatctcgcg catcgagaag accggcgacc cgtggctaga
    25201 gcgcatcgtc cagaaccacg tcgcgatcat cgagatgtgc ctcggcaact tcacgctcgc
    25261 gatgacgtcg gcccaacgct cgctcgagct ctgccgccgc tacggcgatc gatcgcgcga
    25321 aggcgatgcg ctgagcgtcg ccggcatcat cctcctcgaa gtcggcctct acga
//
LOCUS       DATLBZ010000137        15067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5350650, whole genome shotgun sequence.
ACCESSION   DATLBZ010000137 DATLBZ010000000
VERSION     DATLBZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15067
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5350650"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..1670)
                     /locus_tag="VMZ53_07410"
     CDS             complement(234..1670)
                     /locus_tag="VMZ53_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HUS28318.1"
                     /translation="MDAVRRSASSDLIVQVLERELGSDAIVGPGHEKLDDYGRDESPL
                     PEFYPPECAVLCSSTEQVSTVLRLCAEHKVPVTPRGAGSGMVGGALPIAGGIVLSTER
                     MQSIKEIDPADLVCVVEPGVVTGRLQEAVEEQGLFYPPDPASLDFCSIGGNASTNAGG
                     PRAFKYGVTREYVLGLEVGLMGGEVLRCGRRTSKGVTGYDVVAGFVGTEGTFGVITEL
                     TLKLLPNPPAAATVLGVFKDVPTAGTAISSLLRGGLRPRVLELADKHSIDHVRKKARY
                     RFPDNAGAIVLIEVDGQPEEMAAMMEKIGLACDDLGALDVLGATEPAERRAVWEARRL
                     ISSSLKEAWRIKVNEDVCVPRGRIVEMLARIDRVSAETGIATAVFGHAGDGNLHVNLL
                     SNDDPQDPAVRKAMWAAAERVFAHTVELGGTLSGEHGIGLTKRDYMHMEQSEQLLGWQ
                     RKWKAMWDPTGLLNPGKLIPDRKTVCTE"
     gene            complement(1642..2028)
                     /gene="erpA"
                     /locus_tag="VMZ53_07415"
     CDS             complement(1642..2028)
                     /gene="erpA"
                     /locus_tag="VMZ53_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HUS28319.1"
                     /translation="MSEAALATHDHSNDVPAGPESLLRITPVAATKVNEIRGAEGIED
                     NMALRLRVVGGGCAGFSYDLYFDEPAEVDRQFEVQGVRCVVDEMSLMYLVGTEIDYVE
                     GLQGAGFKFQNPNVKSTCGCGSSFSV"
     gene            2097..3260
                     /gene="moeB"
                     /locus_tag="VMZ53_07420"
     CDS             2097..3260
                     /gene="moeB"
                     /locus_tag="VMZ53_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HUS28320.1"
                     /translation="MPGYDALLQELRSKTPEIDVAGLDGVRGSAAVIDCREADEFAQG
                     AIPGAAFIPRGFLESRIEKHVADRDAAIVVYCQSGNRSLFAQRTLTELGYTNVRSLAG
                     GFSAWKRAGLPWEMPKTLRADQETRYSRHVLLPEVGLAGQQKLLASKVVCIGAGGLGS
                     PSSMYLAAAGIGTLGIIDDDVVDASNLQRQILHGTDRLQTPKVESAEKTLKNLNPDVV
                     VEKHRVRLTSANAIEILSRYDVIIDGADNFATRYLVNDVALRLGKPVIHASIFRFEGQ
                     LTVFPANGSPCYRCLYPEPPPPEDAPSCAEGGVLGVLPGSIGVLQATEAIKLILGIGE
                     PLAGRLLVYDALKARFRELKLRRDPKCPTCGEGVDRSAIPLVDYEQFCNAPRR"
     gene            3300..5075
                     /locus_tag="VMZ53_07425"
     CDS             3300..5075
                     /locus_tag="VMZ53_07425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28321.1"
                     /translation="MIAAGQTIGSYRILQKIGTGGMGAVYLAEHPLIGKKVALKVIHK
                     ELATNKEVVQRFFQEAKAVTKIGNEHIIQIHDFGVTPDGDHFYIMDYLDGQTLAQIIA
                     KDTVLPTMRALHIGAQIAAALSAAHSAGVIHRDLKPDNVMLTQRLGDSDFVVLLDFGL
                     AKMNIGGSSAVKTAAGVLLGTPQYMSPEACESRGNLDHRTDIYALGILLFQMITGMLP
                     FDGETMGEVLVKQVTQLPPPPRGFNPSIPPSVEQIVLRCLAKNPDARFPTMISLREAL
                     LDPEQYLRASPPMSPARSLAPGEVKVDAKTIMAYAAQEQQQRTQIGTGSAPLPLPAPP
                     TTLPGNAGAKTMMADGNLVPPRPVQAPPPEMVVPNQPKMNTMRIATPHGYSSRPPRKM
                     WPIVLVLALLLGLGGGAFAVAYFGNSSGTGGGSGGDSGSAVAGGSSGSTGSGPGSAVA
                     VTPIDAAAVAVVAVDAAAPVAAIDAAAAPVVVDAAAAVHVPSGPTAVISIESTPSGAE
                     VFGPDGKSVGKTPVKVTLPISDLPLEFELKLAGYKKKKKSIVVTGNSIVDVPLDRAPS
                     GNSGGNRNKGSGAGNDNGLMNPDDL"
     gene            5102..5845
                     /locus_tag="VMZ53_07430"
     CDS             5102..5845
                     /locus_tag="VMZ53_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HUS28322.1"
                     /translation="MQTDQLASQKSGDAQSPVIVTVPQAHRIARTISFGAISLGSAVG
                     LGAGVAVLMGSRRAGIITGGLTAVAALAFRWQLQRLFTDEPAYEIERRIGDLEIRRYE
                     PRVEAHVRVTAVDFEDALFTGFRQLFRFIDGGNSRGEKLQMTTPVISHPRAMTHTIAF
                     VMPAGRSLASLPASEDIELVEQPVRRIAVLRYRGSYTNENFQEAQAELRDLVADAGLE
                     GAGEPVFAGFDPPTTLPVLRRSEVWIELG"
     gene            5860..7437
                     /locus_tag="VMZ53_07435"
     CDS             5860..7437
                     /locus_tag="VMZ53_07435"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HUS28323.1"
                     /translation="MLIAVIAGVVALVVAAVIAARAAKAEMLRAASSAIDDARMQTAA
                     EIKAAQLEATAKAKEMETAARSEALDAIAKADDAMAADEARATKREDELDKKRDEVAQ
                     VLDAVEQREAQLAQVQKDVQSRFDRARGVDQDIQRKRSSWRGELEKRAGVAGSELITL
                     MTREWVEQAKAKAAAQIRVAEQNTTDPQHDREAKRVMEIAASRCSMHFLTERNLSTLR
                     VGPDIVSILLDNNAAIHTALEKVAGVQLQINDDRDVVRLEGLDGVGREVVRRALARMI
                     KKPETIDNARRSPDEWATKAREHLEQEIRALGKKAFSALGIPKAHPEIVELVGALNFR
                     TSYTQNQWLHAVEASYLAGMMAAELGLDEKLARRATLMHDIGKALTHKIEGSHAVIGA
                     EIARRLGESELVANAIGSHHADEPANSVYAYLVAASDAMSGARPGARREQTEGFSTKV
                     EDLERIGMQRRGVVHAHAVHGGRELRVYVRERDVDDLAVVEMSTEIANQIAEELTFPG
                     QIKVTVIRAFEATSTAN"
     gene            7605..8786
                     /locus_tag="VMZ53_07440"
     CDS             7605..8786
                     /locus_tag="VMZ53_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HUS28324.1"
                     /translation="MANRDDDMQDERFMQRERERSGASRDDRSNRAFDRDDDRNAGWR
                     SIDRGGMGQSGYGAGREGDRSFDQQRSGFPRSFDERSRERGVDDRFAGRGQRGDESHW
                     GNDMGHGSDLNAMYRSGASSNYQHGNYQSGYQQNQGGYQGGPQHGGYGHQGYAPSYRG
                     GMSSHDQGYQGYSQQQGYSQQQGYPQQGYPQQQGYSQPQHGGGMGMGQHGNFRHQSYS
                     HLGEQNFGHLGPDQGAHMSYGSMGDYNQGNRGPDMNRSSWRDDREAHHESLGQRIKGW
                     FQGHRGKGPSGYQRSDDRIRETVCETLSDDDRIDASNIEVTVKNGEVTLSGTVEDRQT
                     KRLAEDCIEHLSGVKDVQNNIRVMAREMGRTSPVGVGTGSSLETPPTAPSNGTDKRHR
                     A"
     gene            8835..10004
                     /locus_tag="VMZ53_07445"
     CDS             8835..10004
                     /locus_tag="VMZ53_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28325.1"
                     /translation="MGRHVIGLAAFVLAGCGFEHGELSTSSMIDAPMIDAPDAEIDSP
                     AITCTPGFVDLCNATPGPALAIAGADTINTDNDPRCVTVAQAAGSAVCLVYASSVMIG
                     SSASLTATGSRPLAIASASTLAIDGIVDVSSQRVTAQIGPAADYSACAVMTPAEDDAG
                     GAGGGAGASFANVGGDGGTGDTDMGTSADGSAVGGRPGGAATVTFLRGGCRGEAGADN
                     PTSGAQGGDGGHGGGALYLFAHDQVAISGIVRATGAGGVGGTIMPTGSGGGGGGGGSG
                     GLVVIESAAIAISGQISANGGGGGEGAFVGGNGGGPLDGRGGDDGVVGTTAAAGGHNT
                     GYVGTGTGGAGAAGTTTAVNGGNSVAGGGGGGGAAGRVKLIGSSTISGIVSPPAS"
     gene            10144..10290
                     /locus_tag="VMZ53_07450"
     CDS             10144..10290
                     /locus_tag="VMZ53_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28326.1"
                     /translation="MNPFFEDDEDPKKPRPVENHKKREGWAERTESRKDQEADGHRHV
                     HVRC"
     gene            10404..11057
                     /locus_tag="VMZ53_07455"
     CDS             10404..11057
                     /locus_tag="VMZ53_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUS28327.1"
                     /translation="MKLAPLAVLTLLGTTSLAAADTPFEIGARVGGYGFRREGDTSKT
                     LGDPHQQWTECRMNGFGMFGGKQLRGPLFVEAGLDLYSSVDAGEPTDLPIDRTSGLLS
                     TAIGARTQIGKHFRGYAQLGGGLELSRVSVPYGDERIRDSKAMPEGFFGIGLDIRVGS
                     QTTLGSSFRAHVMGNFDYDPARLDMSQGWIAAPSKSEVFDASPDVATQVQFYLRREL"
     gene            11057..12088
                     /locus_tag="VMZ53_07460"
     CDS             11057..12088
                     /locus_tag="VMZ53_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme enzyme"
                     /protein_id="HUS28328.1"
                     /translation="MRAALLLLLAACSTEAPPAWTWDLPAKFPTPIVPADNPMSADKV
                     ELGRHLFYDTRLSANGTQSCASCHVQAHAFTDARATPLGATGEPGVHNAMSLANVAYN
                     SSSTWAHDVAALEDQALLPMFGTTPVEMGNAGREAELLARLAGEPLYAELQPLTTESV
                     TRAIAAFERTIISGDSRFDEFVRGDASALDAAEQRGLALFESDRLGCTHCHGGFNFAS
                     AYAFEGERRTQLFNTGLYDVDGRGAYPVGDQGLVEVTGLASDMGRFRAPTLRNIALTA
                     PYFHDGSAATLDAVIDHYAQGGVHSPLQSSFVTGFTLTATERADLIAFLRTLTDESLV
                     ANPAYANPW"
     gene            12113..12727
                     /locus_tag="VMZ53_07465"
     CDS             12113..12727
                     /locus_tag="VMZ53_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS28329.1"
                     /translation="MELVPCGGCRRHVALVENACPFCGAAVELRARARPLLGRVSRAA
                     IFGVGASAAAACWTSSSPGTTTTSGGGGDQVSNHGSGQPAGDLPVPTPATGFAALYGV
                     CSNAATGQPLPNTRVELIPGVGAGLKSVMCDSQGRYAVSDLAPGEWNVRFWAPNDPGR
                     MAPTSQFLELKAGDSKRVDGTVDVRDWSNIPMPYGAPPQRRRVV"
     gene            12743..13240
                     /locus_tag="VMZ53_07470"
     CDS             12743..13240
                     /locus_tag="VMZ53_07470"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HUS28330.1"
                     /translation="MVETAQHQDRSRALGSTLPAGLGTGSKKPSTDPEAARLHALLEV
                     AYLAASADGKLADEEIQLLVANLQLWLGEELDSKFLLGLFDHLGTKLATEGAQARVDA
                     LAAQLDADSRLVAYRLACVTALCDGEVHDEEMRFLEGIVHAFEIPNAEAQAIFDSLDE
                     ALSAI"
     gene            13249..14415
                     /locus_tag="VMZ53_07475"
     CDS             13249..14415
                     /locus_tag="VMZ53_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28331.1"
                     /translation="MLTRFVPSVAPAREPVGGEPAAIEDVRCVARVRGAGGIRELVVG
                     TDANGNVIVTADRGRLGQRARGELAFHETAASGRVLELDTDALYIFDLQTMEMLPDAP
                     GPNDALYVYGLMYGPYPEFADAEVVAVEGQLTVTRADFLGGLVDGVATTFVYPPDAHG
                     SRCVHALLPGERADVIDTGKGVILALPLVPTWLPECPLSNDLVVAQLVYDVLVALRAD
                     LGVTQQLPVPNRAALEAELVAQGWTIEGDEATRGKKGLLGALRGEKKQLPRQGTLDEL
                     VAEARAALSRMSNVPTPEAHALRRRMQRMQPAQVSPKIPAPVVQPPLPPRAQPVPKVK
                     TDKSEWMKDFVDAHRSPSRPTPRVSAPARAVSHKATPAWMEDFAPTKTNKADDD"
     gene            14484..>15067
                     /locus_tag="VMZ53_07480"
     CDS             14484..>15067
                     /locus_tag="VMZ53_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28332.1"
                     /translation="MSEERPVEPFSDLHVEEGHVRATLLHDPTVEGLDVGLYLDGSGS
                     MKEEYAYTQEQTKRTFWQWLLGEPQPMAKPPTNEVEPQARWMLQYLATKDRNGLLRVA
                     YWATGNSGKGVQVVGEMPGTEVNRYTFPGPQTPGGYTYLKPAIDDYIAYLRSQVPNGA
                     RRGLAVFITDGELHDADQVIALTGELARMIKRGQL"
BASE COUNT         2610 a         4847 c         5169 g         2441 t
ORIGIN      
        1 gccgccgcaa actgcgtatc gccgagcacg cacgcgatca gtgcgcgtgg tgccaaccag
       61 ctcgcgacgg tgctcgcgcc ttgccgcacg acaagcggtc gcagcgctgc aatcagctca
      121 tccacgtcgc aattagaaag gggtcaggca caagttcaca agtaactcag accagcttgg
      181 gcggagttac atgcgtttct tgtgaacttg tgcctgaccc cgttcggaac tgcctactcg
      241 gtgcagacgg tcttgcgatc ggggatcaac ttgccggggt tgagcaggcc ggtgggatcc
      301 cacatcgcct tccacttgcg ctgccagccg agcagctgct cgctctgctc catgtgcatg
      361 tagtcgcgct tcgtcaggcc gatgccgtgc tcgccggaga gcgtgccgcc cagctcgacg
      421 gtgtgagcga acacgcgctc ggcggccgcc cacatcgctt tgcggacggc ggggtcttgc
      481 gggtcgtcgt tcgagaggag gttgacgtgc aggttgccgt cgcctgcgtg accgaagacc
      541 gcggtcgcga tgccggtttc ggcggagacg cgatcgatgc gcgcgagcat ttcgacgatg
      601 cggccgcgcg gcacgcagac gtcttcgttc accttgatgc gccacgcttc tttgagcgag
      661 gacgagatta ggcggcgcgc ttcccagacg gcgcggcgtt cggcgggctc ggtggcgccg
      721 aggacgtcga gtgcgccgag gtcgtcgcag gcgaggccga tcttttccat catcgcggcc
      781 atctcttcgg gctggccgtc gacctcgatg agcacgatcg cgcccgcgtt gtccgggaag
      841 cgatagcgcg cttttttgcg gacgtgatcg atcgagtgtt tgtcggcgag ctcgagcacg
      901 cgtgggcgaa ggccaccgcg gaggagcgag gagatggcgg tgccggcggt cgggacgtct
      961 ttgaagacgc cgaggaccgt ggcggcggcc ggcgggttgg ggagcagctt gagcgtcagc
     1021 tccgtgatca cgccgaacgt gccttcggtg ccgacgaagc cggcgacgac gtcgtagcca
     1081 gtgacgcctt tgctcgtgcg acggccgcag cgcagcactt cgccgcccat caagccgact
     1141 tcgaggccca ggacgtattc gcgcgtgacg ccgtacttga atgcgcgcgg gccgccggcg
     1201 ttcgtcgatg cgttgccgcc gatcgagcag aagtcgagcg acgcggggtc gggcgggtag
     1261 aagaggcctt gttcctcgac ggcttcttgg aggcggcccg tgacgacgcc cggctcgacg
     1321 acgcacacga ggtcggcggg atcgatctct ttgatcgact gcatgcgctc ggtcgagagc
     1381 acgatgccgc ccgcgatcgg gagagcgccg cccaccatgc cgctgcctgc gccgcgcggc
     1441 gtgaccggaa ctttgtgctc ggcgcacagg cgaagcaccg tcgagacttg ctcggtcgag
     1501 ctgcagagga ctgcgcactc cggcggatag aactcgggca gcgggctctc gtcacgcccg
     1561 tagtcgtcga gcttttcgtg gccgggcccg acgatcgcgt ccgatccgag ctcgcgctcg
     1621 aggacctgga cgattagatc gctagacgct gaacgacgaa ccgcatccac aggtcgactt
     1681 tacattcgga ttctggaact tgaacccggc gccctgcaga ccttcgacgt aatcgatctc
     1741 cgtgcccacg agatacatga ggctcatctc gtcgacgacg cagcgaacgc cttgcacttc
     1801 gaactgacga tcgacttcgg ccggctcgtc gaaatagagg tcgtacgaga agccggcaca
     1861 gccgccaccg acgacgcgaa ggcggagagc catgttgtcc tcgatgcctt ccgcgccgcg
     1921 gatttcgttg accttcgtag cggccaccgg cgtgatgcgg aggagtgact ccggaccggc
     1981 cgggacgtcg ttcgagtggt cgtgcgtagc gagagctgct tcagacatcg cgagggacct
     2041 taccctcacg tgagggtctg agcaagcgct tgcgtcaatt gtgctaaaac cgcgacgtgc
     2101 ctggctacga cgcactgctc caagagcttc ggagcaagac gcccgagatc gatgtcgcgg
     2161 ggttggatgg tgtgcgcgga agcgcagccg tcatcgattg ccgagaggcg gacgagtttg
     2221 cgcagggcgc catccctggc gccgccttca tcccgcgcgg gtttctggag tcgcgcatcg
     2281 agaagcacgt cgcggatcgc gatgcggcga tcgtcgtgta ctgccagagc gggaatcggt
     2341 cgctgtttgc gcagcggacg ctgaccgagc tcggctacac gaatgttcga tcgctcgccg
     2401 gcggattcag cgcgtggaag cgtgccggcc tgccgtggga aatgccgaag acgcttcgcg
     2461 ccgatcaaga gacgcgctac tcgcggcacg tgttgttgcc cgaggtcggg ctcgcaggtc
     2521 agcagaagct gctcgcgtcg aaggtcgtgt gcatcggggc aggtggcctc ggctcgccgt
     2581 cgagcatgta tctcgcggcg gcgggcatcg gcacgctcgg catcatcgac gacgatgtgg
     2641 tcgacgcgtc gaacctgcag cgtcagatcc tgcatggcac cgatcgactg cagacgccga
     2701 aagtcgagag cgccgagaag acgctcaaga atctgaatcc cgacgtcgtc gtcgagaaac
     2761 accgcgtgag gctcacgagc gcgaacgcga tcgagattct ctcgcgctac gacgtgatca
     2821 tcgacggcgc cgataacttc gcgacgcgct acctcgtcaa cgatgtcgcg ctccggctcg
     2881 gcaagcccgt cattcatgcc tcgatctttc gcttcgaggg ccagctgacg gtgttcccgg
     2941 cgaacggctc gccttgctat cgctgcctgt atccggagcc gccgccgccc gaagatgcgc
     3001 cgtcgtgcgc ggagggtggc gtgctcggcg tgctgcccgg ctcgatcggc gtcttgcagg
     3061 cgaccgaggc catcaagctg attctcggca tcggcgagcc gctcgcgggg cggcttctcg
     3121 tctacgacgc gctcaaggcg cggtttcgcg agctcaagct gcggcgcgac ccgaaatgcc
     3181 cgacgtgcgg tgagggcgtc gaccgctcgg cgatcccgct cgtcgattat gagcagttct
     3241 gcaacgcgcc tcgtcgctag cacaactacg cgacacgact gcgctacaat gagcgcgcgt
     3301 tgatcgccgc cggccaaacg atcggcagct accgcatcct ccagaagatc gggacaggcg
     3361 gcatgggagc cgtctatctc gccgagcacc cgctgatcgg caagaaggtc gcgctcaagg
     3421 tcatccacaa ggagctcgcg acgaacaagg aagtcgtcca gcgcttcttc caagaggcga
     3481 aggccgtcac caagatcggc aacgagcaca tcatccagat ccatgacttc ggtgtgacgc
     3541 ccgacggcga ccacttctac atcatggatt atctcgacgg tcagaccctc gcgcagatca
     3601 tcgcgaagga caccgtcctg ccgacgatgc gcgcgctgca catcggtgcg cagatcgcag
     3661 cggcactctc agcggcgcat tccgccggcg tcatccatcg cgatctcaag cccgacaacg
     3721 tgatgttgac gcagcgcctc ggcgacagcg acttcgtcgt gctcctcgac ttcggcctcg
     3781 cgaagatgaa catcggcggc tcgagcgcgg tgaagaccgc ggcgggcgtg ttgctcggaa
     3841 cgccgcagta catgtcgccc gaggcgtgcg agagccgcgg caatctcgat caccgcacgg
     3901 acatctacgc gctcggcatc ctgctgttcc agatgatcac gggcatgttg ccgttcgacg
     3961 gcgagacgat gggcgaagtg ctcgtcaaac aagttacgca gctgccgcca cctccgcgcg
     4021 gcttcaaccc gagcatcccg ccgagcgtcg agcagatcgt gctgcgctgc ttggcgaaga
     4081 atcccgacgc gcgtttcccg acgatgattt cgctgcgcga ggcgctcctc gatccagagc
     4141 agtacctgcg tgcgtcgccg ccgatgtctc ccgcgcggtc gctcgcgccc ggcgaagtga
     4201 aggtcgacgc caagacgatc atggcgtacg ccgcgcagga gcagcagcaa cgcacgcaga
     4261 tcgggaccgg cagcgcgccg ctgcctctgc ctgcaccacc gacgacgctc cccggcaacg
     4321 caggcgcgaa gacgatgatg gccgacggca acctcgtgcc gccgaggccg gtgcaagcgc
     4381 cgccaccgga gatggtggtg cccaatcagc cgaagatgaa cacgatgcgg atcgcgactc
     4441 cgcacgggta ttcgtcgcgg ccgccgcgca agatgtggcc gatcgtcctc gtgctcgcgc
     4501 tgctgctcgg gctcggcggc ggtgcattcg cggtcgcata cttcggcaac tcgtcgggca
     4561 ctggcggtgg cagcggcggc gactcgggca gcgccgtcgc aggtggctcg agtgggtcga
     4621 ctggctccgg acccggctcg gcggtcgcgg tgacgcccat cgatgctgcc gctgttgccg
     4681 tcgtggctgt cgatgccgca gcgccggtcg cagcgatcga tgcggccgca gcgccggtgg
     4741 tcgtcgacgc agcagcagcg gtgcacgtgc catcgggccc gaccgctgtc atctcgatcg
     4801 agagcacgcc gagcggcgcc gaagtcttcg gccccgatgg caagtccgtc ggcaagacgc
     4861 cggtgaaagt cacgctgccg atttcggact tgccgctcga gttcgagctg aagctcgctg
     4921 gttacaagaa aaagaaaaaa tcgatcgtcg tgacgggcaa ctcgatcgtc gacgttccgc
     4981 tcgatcgcgc gccttcgggc aacagcggtg ggaatcgcaa caagggtagc ggcgccggca
     5041 acgacaacgg cctgatgaac cccgacgatc tataggcacg cagcttgcga ccgatcggta
     5101 catgcagacc gatcagttgg cttcgcagaa atccggtgac gcgcagtcgc ccgtcatcgt
     5161 gacggtgccg caagcgcatc gcatcgcacg cacgatcagc ttcggcgcga tctcgctggg
     5221 cagtgcggtc gggctgggcg ctggtgtcgc agtgctcatg ggctcgcgtc gcgcaggcat
     5281 cattaccggc gggctcacgg cggtcgctgc actcgcgttc cgctggcagc ttcagcggtt
     5341 gttcaccgac gagccggcgt acgaaatcga acgacgcatc ggtgacctcg agattcgccg
     5401 gtacgagccg cgcgtcgagg cgcatgttcg cgtcactgcg gtcgacttcg aggacgcgct
     5461 gttcacggga tttcggcagc tctttcgctt catcgacggc ggcaactcgc gcggcgagaa
     5521 gctgcagatg acgacgcccg tgattagtca tccgcgcgcg atgactcaca cgatcgcatt
     5581 cgtgatgcct gccggacgct cgctcgcgtc gctgccggcg tcggaagaca tcgagctcgt
     5641 cgagcaaccg gtgcggcgca tcgcggtgct gcgctatcgc ggcagctaca cgaacgagaa
     5701 tttccaagag gcgcaagccg agctgcggga cctcgtcgcg gatgcgggcc tcgagggtgc
     5761 cggcgagccg gtcttcgcgg gcttcgaccc accgacgaca ctgcccgttc tccgtcgaag
     5821 tgaggtgtgg atcgagctgg gctgacgtaa gcttggggca tgctgatcgc ggtcatcgcc
     5881 ggtgtcgtcg ccctcgtggt ggccgccgtg atcgccgcgc gtgccgcgaa ggcggagatg
     5941 ctccgcgccg ccagcagcgc catcgatgac gcgcggatgc agaccgctgc ggagatcaag
     6001 gccgcgcagc tcgaggcgac ggcgaaggcc aaggaaatgg agaccgccgc gcggtccgaa
     6061 gcgctcgacg cgatcgcgaa ggcggacgat gcgatggccg ccgacgaagc gcgcgctacc
     6121 aagcgcgagg acgagctcga caagaagcgc gacgaggtcg cgcaggtcct cgatgccgtc
     6181 gaacagcgcg aagcgcagct ggcgcaggtg cagaaagatg tgcagtcgcg attcgatcgc
     6241 gcgcgcggcg tcgaccaaga catccagcgc aagcggtcgt catggcgtgg cgagctcgag
     6301 aagcgcgccg gtgttgcggg cagcgagctg atcacgctga tgacacgcga gtgggtcgag
     6361 caggcgaagg cgaaagccgc cgcgcagatc cgcgtcgccg agcagaacac gacggatccg
     6421 cagcacgacc gcgaagcgaa gcgcgtcatg gagatcgcgg cttcgcgctg cagcatgcac
     6481 ttcttgacgg agcgcaacct ctccacgctg cgcgtcggtc ccgacatcgt ctcgatcctc
     6541 ctcgataaca acgcggcgat tcacacagcg ctcgagaagg tcgcgggcgt gcagctgcag
     6601 atcaacgacg atagagatgt cgttcggctc gagggtctcg acggcgtcgg ccgcgaagtc
     6661 gtgcgccgcg cgctcgctcg catgatcaag aagcccgaga cgatcgacaa cgcccgcagg
     6721 tcgccagacg agtgggccac caaggcgcgc gagcatctcg agcaagagat ccgtgcgctc
     6781 ggcaagaagg cgttctcggc gctcggcatc ccgaaagcgc atcccgagat cgtcgagctc
     6841 gtcggtgcgc tgaacttccg cacgagctac acgcagaacc agtggctgca tgccgtcgag
     6901 gcgagctacc tggcaggcat gatggccgcc gagctcggtc tcgacgagaa gctcgcacgt
     6961 cgggccacgc tgatgcacga catcggcaaa gcgctcacgc acaagatcga gggcagtcac
     7021 gccgtcatcg gtgcggagat cgcgcgccgc ttaggcgagt cggagctcgt cgcaaatgcg
     7081 atcggctcgc atcacgcgga cgagccggct aactctgtct atgcgtatct cgtcgctgcg
     7141 agcgacgcga tgagtggtgc gcgtcctggt gcgcgtcgtg agcaaaccga aggcttctcg
     7201 acgaaggtcg aagatctcga gcggatcggc atgcagcggc gcggtgtcgt gcatgcgcat
     7261 gcagtgcatg gcggccgcga actgcgcgtc tatgtgcggg agcgcgatgt cgatgatctc
     7321 gcggtcgtcg agatgtcgac ggagatcgcc aatcagatcg ccgaagagtt gacgtttccc
     7381 ggccagatca aggtcaccgt gattcgcgcg ttcgaagcga cgtcgactgc gaactgagcc
     7441 gccggagcgg gcggtctcgc cacagcgcac gccggttacg ggcggctcgg catgcgtccc
     7501 acatttccac cgagcgggag cttcccgcat cgctgattgg cgcgtgcgct gcggggcaca
     7561 ctcgatgcac gacgcgggca ggcactcaca gaggggattc gaacatggcg aaccgtgacg
     7621 acgatatgca ggatgaacgc ttcatgcagc gcgagcgcga gcgctccggt gcgagcaggg
     7681 atgatcgctc taatcgcgcg ttcgatcgcg acgacgatcg caacgccggc tggcgcagta
     7741 tcgatcgcgg cggtatgggg cagagcggct acggcgccgg acgcgaaggt gatcgctcct
     7801 tcgatcagca acgctcgggc tttccgcgca gcttcgatga gcgctcacgc gagcgtggtg
     7861 tcgacgaccg cttcgccggt cgcggccagc gcggcgacga gagccactgg ggcaacgata
     7921 tgggccacgg ctcggacctc aacgcgatgt accgctcggg tgcgagctcg aactatcagc
     7981 acggcaacta tcagagcggc tatcagcaga accaaggtgg ctatcaaggc ggaccgcaac
     8041 acggcggcta tggccaccag ggctacgcgc cgagctatcg cggcggcatg agcagtcacg
     8101 accagggcta ccaaggctac tcgcagcagc aaggctactc gcagcagcag ggttacccgc
     8161 agcagggtta cccgcagcag cagggctact cgcagccgca acatggtggc ggcatgggca
     8221 tgggccagca cggcaacttc cgccatcagt cgtacagcca tctcggcgag cagaacttcg
     8281 gccacctcgg tccggatcaa ggcgcgcaca tgagctacgg cagcatgggc gactacaacc
     8341 agggcaatcg cgggcccgac atgaatcgca gctcgtggcg cgacgatcgc gaagcgcatc
     8401 acgagtcgct cggccagcgg atcaaaggct ggttccaagg tcatcgcggc aaaggcccgt
     8461 cgggctatca gcgctctgac gaccggatcc gcgagacggt gtgcgagacg ctcagcgatg
     8521 atgatcgcat cgacgcatcg aacatcgagg tcaccgtcaa gaacggtgag gtgacactgt
     8581 cgggcaccgt cgaggatcgc cagaccaagc ggctcgccga ggactgcatc gagcatctct
     8641 cgggcgtcaa ggacgtgcag aacaacatcc gcgtcatggc gcgcgagatg ggtcgtacgt
     8701 cgccggtcgg cgtgggcacg ggcagctcgc tcgagacgcc tccgacggcg ccgagcaacg
     8761 gcacggacaa gcgccaccgc gcctgaacgc tttcgagcac gcgaggccgc tgatgcggcg
     8821 tatcatcgga aacgatggga cgtcacgtca tcggcctcgc cgcgtttgta cttgcgggct
     8881 gtgggttcga gcacggcgag ctgtcgacgt cgtcgatgat cgacgcgccg atgatcgacg
     8941 cgcctgatgc cgagatcgat agcccggcga tcacgtgcac gcccgggttc gtggatctgt
     9001 gcaacgcgac gccgggtcct gcgctcgcga ttgcgggtgc tgacacgatc aacacggaca
     9061 acgatccgcg ttgcgtgacc gtggcgcagg cggcgggctc ggccgtgtgt ctcgtctacg
     9121 ccagctccgt gatgatcggt tcgagcgcgt cgctcacggc gaccggttcg cgcccgctcg
     9181 cgatcgcatc ggcatcgacc cttgcgatcg atggcatcgt cgacgtgtcg agtcagcgcg
     9241 tgacggcgca gatcggtccc gctgccgact acagcgcgtg cgcggtgatg acgcccgcgg
     9301 aggatgacgc aggaggtgcc ggaggcggtg cgggtgcgag cttcgcgaac gtgggcggag
     9361 atggcggcac tggtgataca gacatgggca caagcgccga tggttccgct gtcggtgggc
     9421 gcccaggcgg cgcggccacg gtgacgttct tgcgtggagg ttgtcgaggc gaggcgggcg
     9481 cagacaaccc gacgagcggc gcacaaggcg gcgacggtgg acacggcggc ggtgcgctgt
     9541 acctgttcgc gcatgatcaa gttgcgatct cgggcatcgt gcgcgcgacg ggcgccggtg
     9601 gcgtcggcgg cacgatcatg ccgaccggaa gcggcggcgg tggcggagga ggcggcagcg
     9661 gcgggctcgt cgtgatcgag agcgcggcga tcgctatcag cgggcagatc agcgcgaacg
     9721 gcggcggggg cggcgagggt gcgttcgtcg gtggcaatgg cggcgggcct ctcgacggcc
     9781 gtggcggcga tgacggcgtc gtcggtacga cggcggctgc cggcgggcac aacacgggct
     9841 acgtcgggac cggcaccggt ggggcaggcg cggcaggtac gacgaccgcg gtcaacggcg
     9901 ggaactcggt cgcaggcggc ggcggtggcg gcggtgcggc cggccgggtc aagctcatcg
     9961 gatcctcgac gatatcgggc atcgtttcgc cgcccgcgag ctaggcctca tcgaagctcg
    10021 gacgcgtagc acgcgggcac gctgtggcgg cgcgcgcgtc gtgcgccgcc gtcgctgtcg
    10081 tcgagtatgc aatcctttct acaggttcgc gttggcacct gcgatgcaca attcgtcgtc
    10141 gctatgaacc cgtttttcga ggacgacgag gatccgaaga agccgcgtcc cgtcgagaat
    10201 cacaagaagc gtgaaggctg ggcggagcgc accgagtctc gcaaggacca ggaagccgac
    10261 ggccaccgtc acgtccacgt tcgctgctga gcaggtgcgg gaaatagcga cggtcgctgt
    10321 cgccaatccc cacaaaccgc agccgggcgt tcacaggtcg agaacggccg ttcgcggcga
    10381 gggcattgct cttgcggcgg accatgaagc tcgcgcccct cgcagtcctg accctgctcg
    10441 gaacaacctc gctcgctgcc gctgatacgc cgttcgagat cggcgcgcgt gtcggtggct
    10501 acggctttcg ccgcgaaggc gacacctcca agacgctcgg cgatccgcac cagcagtgga
    10561 ccgagtgccg gatgaacggc ttcggcatgt tcggcggcaa gcagctgcgc gggccgctgt
    10621 tcgtcgaagc cggcctcgac ctctacagct cggtcgatgc gggcgagccc acggacctgc
    10681 cgatcgatcg cacgagcggt ctgctctcga cggccatcgg tgcccgcacg cagatcggca
    10741 agcacttccg cggctacgcc cagctcggcg gcggtctcga gctgtctcgc gtgtcggtgc
    10801 cgtatggcga cgagcgcatc cgcgacagca aggcgatgcc cgaaggcttc ttcggcattg
    10861 gtctcgacat tcgcgtcggc tcgcagacaa cgctcggctc gagcttccgc gcgcacgtga
    10921 tgggcaactt cgactacgac cctgcgcgac tcgacatgag ccagggctgg atcgcagcgc
    10981 cgagcaagag cgaagtgttc gacgcatcgc ccgacgtcgc gacccaagtg cagttctatc
    11041 tccgccgcga gctgtgatgc gcgctgcgtt gctgttgctg ctcgccgcgt gctcgacgga
    11101 agcgccgccc gcctggacgt gggacttgcc ggcgaagttt ccgacgccga tcgtgcccgc
    11161 ggacaacccg atgtcggcgg acaaggtcga gctaggtcgc cacctgttct acgacacgcg
    11221 gctgtcggcg aacgggacgc agtcctgcgc aagctgtcac gtgcaggcgc acgcgttcac
    11281 cgacgctcgc gcaacgccgc tcggggcaac gggagagccc ggcgtgcaca acgcgatgag
    11341 cctcgcgaac gtcgcgtaca acagctcgag cacgtgggcg catgacgtcg ccgctctcga
    11401 ggatcaggcg ctgctgccga tgttcggcac gacgcccgtc gagatgggca acgcgggtcg
    11461 cgaagccgag ctcctcgcac gcctcgccgg cgagccgctg tacgcggagc tgcagccgct
    11521 cacgacggag agcgtgacgc gcgcgatcgc ggcattcgag cggacgatca tctcggggga
    11581 ctcgcgcttc gatgagttcg tgcgcggcga tgcatcagct ctcgacgcag cggagcagcg
    11641 tggtctcgcg ctcttcgaat cagaccggct cggctgcact cattgtcacg gtggcttcaa
    11701 cttcgcgtcg gcgtacgcgt tcgagggcga gcgccgaacg cagctgttca acacaggcct
    11761 ctacgacgtc gacgggcgcg gcgcgtatcc tgtcggcgac caaggcctcg tcgaggtgac
    11821 cggcctcgcg agcgacatgg gccggttccg cgcgccgacg ctgcgcaaca tcgcgctgac
    11881 cgcgccgtac ttccacgacg gcagcgcggc gacgctcgac gcggtgatcg atcattatgc
    11941 gcaaggtggc gtgcacagcc cgttgcagtc gtcgttcgtc acgggcttca cgctgaccgc
    12001 cacagagcgc gcggatctca tcgccttcct tcgcacgctg accgacgaat cgctcgtcgc
    12061 taatcccgcg tacgcgaacc cttggtgagt gctgccctgt tacgctcgct ctatggagct
    12121 cgttccgtgt ggtgggtgca ggcgacacgt cgcgctcgtc gagaatgcgt gtccgttttg
    12181 cggtgcggcg gtggagctac gagctcgcgc gcggccgttg ctcggcaggg tgagtcgcgc
    12241 agcgatcttt ggcgtcggtg cgagtgcggc ggctgcgtgc tggacgagta gctcgccggg
    12301 gacgaccacc acgagcggcg gcggcggcga tcaggtgtcg aaccacggct cgggccagcc
    12361 agccggcgac ctgccggtgc cgaccccggc gaccgggttc gcagcgctct acggcgtctg
    12421 cagcaatgcg gcgaccggcc agccgcttcc gaacacgcgt gtcgagctca tccctggcgt
    12481 tggcgcgggg ctgaagtccg tgatgtgcga ctcgcagggc cgctacgccg tcagcgatct
    12541 cgcgcccggc gagtggaacg ttcggttctg ggcgccgaac gatccggggc gcatggctcc
    12601 cacctcgcag ttcctcgagc tgaaagccgg agacagcaag cgggtggacg gcaccgtcga
    12661 cgttcgcgat tggtcgaaca tcccgatgcc gtacggtgca ccgccgcaac gacgtcgcgt
    12721 cgtctgatgg taaacatacg cgatggtcga aactgctcag catcaagatc gtagtcgcgc
    12781 actcggcagc acgctgcccg ccggtctcgg aaccggatcc aagaagcctt cgactgatcc
    12841 agaggccgcg cgcctgcacg cgctcctcga ggttgcgtac ttggcggcgt ctgcggacgg
    12901 caagctcgcc gacgaggaga tccaactcct cgttgcgaat ctccagctgt ggctcggtga
    12961 ggagctcgat tcgaagttcc tcctcgggtt attcgaccac ctcggtacga agctcgcgac
    13021 cgagggcgcg caagctcgcg tcgacgcgct cgcagcacag ctcgacgccg actcgcgcct
    13081 cgtcgcatac aggctcgcgt gcgtcactgc gctgtgtgat ggcgaagtcc acgacgaaga
    13141 gatgcggttc ctcgagggca tcgtccacgc gttcgagatt ccgaacgccg aagcgcaagc
    13201 gatcttcgat tcgctcgacg aagcattgtc agcgatctga gagcgcccgt gctgacgcgg
    13261 tttgtgccga gcgttgcgcc tgcgcgcgag cccgtcggtg gtgaaccagc ggcgatcgag
    13321 gacgtacgct gtgtcgcgcg tgtgcgaggg gccggcggta ttcgcgagct cgtcgtcggc
    13381 acggacgcga atggcaacgt gatcgtgacg gccgaccgag gcaggctcgg ccagcgagcg
    13441 cgcggcgagc tcgcattcca cgagacggcc gcatccggtc gcgtgctcga gctcgatacc
    13501 gatgcgctct acattttcga tctgcagacg atggagatgc tgcccgatgc gccgggcccg
    13561 aacgacgcgc tctacgtcta tgggctgatg tacgggccct atcccgaatt tgccgacgcc
    13621 gaggtcgtcg ccgtcgaggg acagctcacg gtcacgcgcg cagattttct cggcgggctc
    13681 gtcgacggcg ttgcgacgac gttcgtgtat ccgcccgatg cgcacggctc gcgctgcgtg
    13741 cacgcgctgc tgcccggcga gcgcgcggat gtgatcgaca ccggcaaggg cgtcatcctc
    13801 gcgctgccgc tcgtgccgac ctggttgccg gagtgcccgc tgtcgaatga cctcgtcgtc
    13861 gcgcagctcg tgtacgacgt gctcgtcgcg ttgcgcgcgg acctcggtgt gacgcagcag
    13921 ctgccggtgc cgaatcgcgc ggcgctcgaa gccgagctcg tcgcacaggg ctggacgatc
    13981 gaaggcgatg aagcgacgcg cggcaagaag gggttgctcg gtgcgctgcg cggcgagaag
    14041 aaacagctgc ctcggcaggg cacgctcgac gagctcgtcg ccgaagcgcg cgcagcgctc
    14101 tcgcgcatgt cgaacgttcc cactccggaa gcgcacgcgc tgcgcaggcg catgcagcgg
    14161 atgcaaccgg cgcaggtgtc gccgaaaatt cccgcgccgg tcgtccagcc gccgcttcct
    14221 ccgcgcgcgc agccggtgcc gaaagtgaag accgacaaat ccgagtggat gaaagacttc
    14281 gtcgacgcgc atcgctcgcc gtcgcgtccc acgccgcgtg tctctgcacc cgcaagagcc
    14341 gtctcgcaca aagcgacacc agcgtggatg gaagactttg ctccgacgaa aacgaacaaa
    14401 gccgacgacg actagtgccg atcatcgctt gctgtccctc gcgtgcatcg ttacgttgat
    14461 cgcccgtcag gaggcgatgg ttcatgagtg aagaaagacc ggtcgagccg ttctcggatc
    14521 tgcatgtcga agagggtcac gtgcgcgcga cgctgctgca cgatccaacc gtcgagggcc
    14581 tggatgttgg cctctatctc gatggctccg gcagcatgaa agaggagtac gcgtacacgc
    14641 aggagcagac gaagcgcacg ttctggcagt ggctccttgg cgagcctcag ccgatggcga
    14701 agccgccgac gaatgaagtc gagccgcaag cgcggtggat gctgcagtac ctcgcaacga
    14761 aggatcgcaa cggtctgctt cgcgtcgcgt attgggcgac cggcaacagc ggcaagggcg
    14821 ttcaggtcgt cggcgagatg ccgggcaccg aagtgaatcg gtacacgttc cccggcccgc
    14881 agacgccagg tggctacacc tatctcaagc ccgcgatcga cgactacatc gcgtatctgc
    14941 gctcgcaggt cccgaacggg gcacgtcgcg gtctcgcggt gttcatcacc gacggtgagc
    15001 tgcacgacgc agatcaagtc atcgcgctca ccggtgagct cgcgcgcatg atcaagcgcg
    15061 gtcagct
//
LOCUS       DATLBZ010000138         2073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5351333, whole genome shotgun sequence.
ACCESSION   DATLBZ010000138 DATLBZ010000000
VERSION     DATLBZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5351333"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1017
                     /locus_tag="VMZ53_07485"
     CDS             121..1017
                     /locus_tag="VMZ53_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS28333.1"
                     /translation="MNLLFCGSGWLPIVDHIAARLGDAHSITRWDLVTPLALVVRKAD
                     VLLPSNAHITPEVITSATRLRLIQQPAAGTEKIDRAAAQARGIPICNAPGTNHVSVAE
                     HALFLLLALARRAPLAARAFAAREVGTPLGIELAGKTLGIIGMGRSGTALAERATALG
                     MHVRPLHRGANAGEQRAFFAACDAISIHCPLTEQTRGLVGRDALGAMKPGALLVNVAR
                     GGVIDRDALVAALPKLGGVGLDVHWSEPPDPDDPLYAQPNVIGLPHVAGSTEEAFARI
                     TDVVIENLARLQRGDALVNRLV"
     gene            complement(1018..1821)
                     /locus_tag="VMZ53_07490"
     CDS             complement(1018..1821)
                     /locus_tag="VMZ53_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28334.1"
                     /translation="MRWFVLLGVLGGCDTVFSVDHVGPTRDDARGADAALDAAPGCWS
                     AFQQDHDEDGDGRMDGCDNCPAVFNEQQEDDDGDQVGNACDPHPGMPDRIVYFNGFES
                     TARGFTTRNGAWTISNDTFVQSDVARAYAFLEGEFTGATVEVRLQGVTQPATGADHAG
                     AYVAATAVDLDPPHGLRCYEAKVAGDSDQSVIENVGTTQVTILPIDGPKPFYMRLSVQ
                     GANSRPLCRTSRGNGDVDVTFSNLVAQTGSIALETTNSACAFLSVTVMN"
     gene            1888..>2073
                     /locus_tag="VMZ53_07495"
     CDS             1888..>2073
                     /locus_tag="VMZ53_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28335.1"
                     /translation="MTWLVRYLRSSIGAKHVMAVTGLLLLLFAIVHMIGHLQMFGGRD
                     MYNDYASFLQNLWEVKWP"
BASE COUNT          346 a          690 c          656 g          381 t
ORIGIN      
        1 catccccaca gcgagaacga tgctgtgcaa catcgaggtt gcagctgcct tgtcatttcg
       61 ctgagttgga cgtcgagagc gcatgtgcgc agggcattca gcaagtacga tgcccaggtc
      121 gtgaacctct tgttctgcgg gtctggctgg ctgccgatcg tcgaccacat cgcggcacgt
      181 cttggtgacg cgcactcgat cacgcgctgg gacctcgtca cgccgctcgc gctggttgtc
      241 cgaaaagcgg acgtcttgct gccgtcgaac gcgcacatca cgcccgaggt catcactagc
      301 gcgacgcgcc tccggctcat ccagcagccc gcggcgggaa ccgagaagat cgatcgcgca
      361 gctgcacagg cgcgcggaat tccgatctgc aatgcccccg gaacgaatca cgtctcggtc
      421 gcagagcatg cgctctttct attgctcgcg ctcgcgcgtc gcgcgcctct cgcagcacgc
      481 gcattcgcag cgcgcgaagt cggcacgccg ctcggcatcg agctcgccgg caagacactc
      541 ggcatcatcg gcatgggccg ctcgggcacc gcgctcgcgg aacgcgcaac cgcgctcggg
      601 atgcacgtgc ggcctctgca tcgcggcgcg aatgcgggcg aacagcgcgc cttcttcgca
      661 gcctgcgacg cgatcagcat ccactgcccg ctcaccgaac agacgcgtgg cctcgtcggt
      721 cgcgatgccc tcggcgcgat gaagccgggc gcgctgctcg tcaatgtcgc gcgtggtggc
      781 gtgatcgatc gcgacgcgct cgtcgccgcg ctgccgaagc tcggcggtgt cggcctcgac
      841 gtgcattggt cggagccgcc agatccggac gatccgctgt acgcccagcc gaacgtgatc
      901 ggcctgccgc acgtcgcggg ctcgaccgaa gaggcgttcg cgaggatcac ggatgtcgtg
      961 atcgagaacc tcgcgcggct gcaacgcggc gacgcgctcg tcaaccgact cgtgtgatca
     1021 gttcatcacg gtcacggaca ggaacgcgca cgcactattc gtcgtctcga gggcgatcga
     1081 gcccgtctgc gcgacaagat tactgaacgt gacatcgacg tcgccgtttc cgcggctcgt
     1141 ccggcagaga ggacggctgt tcgcgccttg caccgacaac cgcatgtaga acggctttgg
     1201 cccgtcgatc ggcaggatcg tcacttgtgt cgtgccgacg ttctcgatca cggactgatc
     1261 gctatcgcct gcgacttttg cttcgtagca gcgaagcccg tgcggcggat ccagatcgac
     1321 cgccgtcgcc gcgacatacg cgcctgcgtg atcggcgccg gtggcgggtt gcgtgacgcc
     1381 ttgcaggcga acttcgacgg tcgcgccggt gaattcgccc tcgaggaatg cataggccct
     1441 cgcgacgtcg gactggacga acgtgtcatt cgaaatcgtc catgcgccat tgcgcgtcgt
     1501 gaagccgcga gcggtcgact cgaagccgtt gaagtacacg attcggtcgg gcatacctgg
     1561 atgcggatcg cacgcgttgc cgacttgatc gccgtcgtcg tcttcctgct gttcgttgaa
     1621 gaccgccgga cagttgtcgc aaccgtccat gcggccgtcg ccatcctcgt cgtggtcttg
     1681 ttgaaatgcc gaccagcagc cgggcgcggc atcgagcgcc gcgtcggcac cgcgtgcgtc
     1741 gtcgcgcgtc ggtcctacgt gatcgacgga gaacacggtg tcacagccgc ccaagacgcc
     1801 gagtagcacg aaccaacgca cgcgaccatt gtatccggct caacgcattg ccgcatctac
     1861 cgcacctgtg atggcatctc accactcgtg acctggcttg ttcgctacct gcgatcgtcg
     1921 atcggcgcga agcatgtgat ggccgtgacc ggcctcctgc tgctgctgtt cgcgatcgtc
     1981 cacatgatcg gccatttgca gatgttcggc gggcgcgaca tgtacaacga ctacgcgagc
     2041 tttctgcaga acctgtggga agtgaagtgg ccg
//
LOCUS       DATLBZ010000139         8269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_879942, whole genome shotgun sequence.
ACCESSION   DATLBZ010000139 DATLBZ010000000
VERSION     DATLBZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879942"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VMZ53_07500"
     CDS             complement(<1..342)
                     /locus_tag="VMZ53_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HUS28336.1"
                     /translation="MIAVLDVCSGNLRSVERALAHVGAPVVVTRDPEVVRKADKLVVP
                     GQGAFGVFMRGLAERGLGDALREQIARGTPYLGICLGLQVLFEHSEEGECVGLGVLRG
                     NVTRLVPGDPHL"
     gene            complement(339..938)
                     /gene="hisB"
                     /locus_tag="VMZ53_07505"
     CDS             complement(339..938)
                     /gene="hisB"
                     /locus_tag="VMZ53_07505"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HUS28337.1"
                     /translation="MGRRVKLDRNTKETQISVDLEIDGTGQRTIQTPLPFLSHMLDAF
                     ARHGVFDLAVTAAGDIEIDGHHTVEDIGLVLGNAFEQALGDRAGLVRYGWATLPMDEV
                     MVTVAIDFCGRPAFVWKVQGLEGKWIGGFDCELAKEFFAAFATRAQCNLHVNVHYGGN
                     AHHMIECIFKAFARACDAATRIDPRLGGAIPSTKGTLTE"
     gene            991..2262
                     /locus_tag="VMZ53_07510"
     CDS             991..2262
                     /locus_tag="VMZ53_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28338.1"
                     /translation="MRGYVLVVGCTISLFACGGGGEKKVKKPKPKKEVEETVKAETPE
                     DREKARHDQAVKIVPEGSTCLPASLKEDGAPRLELAASGSEAILCALDEDQNRLLGPV
                     GCWKINLANGELEYKGTDLPPGRGVWVKLDESCAKGYCLKSVKSTKALVAKDLDGKKV
                     AVLAGDDVHLFDAESKEESSSFSIRGDKGLTNDPKGMVFVGDHVAVEGADEGPYSAVW
                     VFKTDGTQLGPVTMLGGKEEKPVSTYKGSLSVLDKNRIAVADHGMETLTTYQLDNGAR
                     GKLVRKLKAKPACKPVELDAYWHEGDKVTDKCKDSIEKGYGLMMGATLVAGAKNNLAL
                     LRGDRLGDLGVLDPKSLEEKKAIKLPWCAADAAGAGGGGGGGGGDADEESKDEAKDAK
                     KDSKESDAAPADKKKGKSKPGDTSDPQEGGQ"
     gene            complement(2354..5074)
                     /locus_tag="VMZ53_07515"
     CDS             complement(2354..5074)
                     /locus_tag="VMZ53_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUS28339.1"
                     /translation="MSDPIVGIDLGTSNTVVAHADTTGQVQVLADLNGFKIHPSVVSF
                     HPTGGVVVGAAAKQRKVIDPKNTIFSVKRLIGRAWNSPEVQTAKQRMPYQIKEGQNQL
                     PLVVTRGGEFAVPEISAIVLDHARTIASTQLKTEVGRAVVTVPASFNDAQRSATATAG
                     SIAGLQVVRVLNEPTAAALAYGNARNLNETIAVYDFGGGTFDITILKLADQVYEVLGT
                     AGNTFLGGDDLDERLVDRMVEKFLAENRVDLRTNEVSMMRLRAVAEQTKIELSRRSRA
                     VVRVDEIAYGAKGKPLDLQIEIRRDEFVKQVADIIDQTFPVCEEALKYANLTIDGIND
                     VILVGGTTKIPYVRDQVSKFFNKAPRTDVNPEDAVAVGAAFQAKALERVLNKKSSVSQ
                     VSGVPSPVGQFEQEESTFGGNETQTFSRGDEERTNESAAPRNDRADTAYSLRGGHHDR
                     LEEKAGPAATPPAGQPVIGRLTKEIKPPPGAKTAPGVAQAAPRTTARGVAQTPPHGTT
                     QPLPPPVPPGKPSRKTAIAMPIVRPPAPPSSPQAKTLVVPERESSTVIVDPAASEPTL
                     QTSENIDFPDTGTEKGFLDLDDSTSPASTARGFGAATGAATRPGPRGPAAVAPTSEDI
                     DFPDTGTEKGFLDLDDSTSPASTARGLGSASSTATSPRPDAFAPPAPPARPGAAPLPP
                     TPPVGAFGSPPPSPTSSPAARTLMLEPSPASQSPTMIPQSPASQSPTMIAPSGLPSGL
                     PPGPLQPPVWPPPSPESAPPTPIGRAGGGSPVIMDVTPRGLGIGTVAGYCEELIRRNS
                     RVPTETRKLFTTSRDHQDAVRIIVCQGESRRLDNNVVIGDLLLQGLPRRPRGETSIEV
                     TFHLDANGILQVRARDAQTGNEQRASLDLVGAMPQEEVAASRERLQALRR"
     gene            complement(5108..5647)
                     /locus_tag="VMZ53_07520"
     CDS             complement(5108..5647)
                     /locus_tag="VMZ53_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28340.1"
                     /translation="MDRYRVLVEALREEIPGFRIVRKDQSALHRAIDVALKVVTFGRM
                     KTYLDSFQTTIGKTVYVTADWDDWEPDDRYVTLRHEAVHLRQFRKLTLPVMAVLYIFV
                     PLPMGVAYFRARFEKEAYAETIRARAEVSGLGYVRGSHHRNYVIEQFTSPSYGWMWPF
                     RRGLERWYDQILATLEDRR"
     gene            5671..7758
                     /locus_tag="VMZ53_07525"
     CDS             5671..7758
                     /locus_tag="VMZ53_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HUS28341.1"
                     /translation="MTDRAQNISVDGSVYVDLSKLNPPQREAASHGGGPILVLAGAGS
                     GKTRVITFRIAHLLGQGVSPRAICAVTFTNKAAEEMRERIAALLGDRELAQQLTIGTF
                     HALGLQILRMERKALGLPRGFAIYDQSDQLGAVREAMRHIHDGDRRFDAKAVLTRISL
                     AKNAFVAPEEYEGNPADDYDAMTAEVYPKYQETLRSCAAFDFDDLIVEPVRLWQRDAE
                     VRSKWASRFQYVMVDEFQDTNKAQLRMVKGLVEQHGNLCVVGDDDQSIYSWRGADPTN
                     ILQFDKMFEGAKVVKLEQNYRSTKTILAAANEVIGNNKDRHGKTLWSDLGGGEIITHA
                     VAPTAEDEAKWIAREIRQLVKEGRRWGDFAVLYRSNIQAKAIEEELRTAEVPYVMYGG
                     QQFFERKEVKDVIAYMRVALNSRDELALRRVINYPARGIGATTIDKLVQHAHATHTTL
                     WEALSNAEEAGVHGAARTAIEELQHIVRTIGAAFDAGDNIVEATKQMIEDIGLYDDLR
                     SAAGSMSAAQRRIDNVTGLLNSLERWAAKGKGQKELAEYLRMLSLDSKDESEKHQDNG
                     EKVVLTTLHGAKGLEFPVCFMIGLEEELLPHIRTLQPQVTDPSDADHATDISEERRLC
                     YVGITRAQRKLYLTRACTRVSRGRILQRTPSRFLLEIPDELLEIRDLGEEARQAVPKE
                     ELKTFFSSFSFDD"
     gene            7810..>8269
                     /locus_tag="VMZ53_07530"
     CDS             7810..>8269
                     /locus_tag="VMZ53_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HUS28342.1"
                     /translation="MSAGVGGGPSVLVGDPVVFAEAMKRKRNAILCGALLAACGGSPK
                     TAPVELGTTHSSGDVVPAKPAVVEAVDPNLAFRLSYSNPGGMWMPAQMTLPQHVETFR
                     KMGVKLPADALANPLSETLTSIVSLGGCSASFVSPDGLVVTNHHCVQGALK"
BASE COUNT         1494 a         2592 c         2810 g         1373 t
ORIGIN      
        1 gaggtgagga tcgccgggca ccaggcgcgt gacgttaccg cgcaacacac cgaggccgac
       61 gcattcgccc tcttcgctgt gctcgaacag gacttgcagc ccgaggcaga tcccgagata
      121 cggcgtgccg cgtgcgatct gttcgcgcag ggcgtcaccc agcccacgtt cggccagccc
      181 gcgcatgaac acgccgaatg cgccctgacc gggcaccacc agcttgtccg ctttccggac
      241 cacttcggga tctcgcgtga cgacgacagg cgcgccgaca tgcgccagcg cgcgctcgac
      301 cgagcggaga ttgccagagc acacgtcgag gaccgcgatc actccgtgag cgtacccttg
      361 gtcgacggaa tcgcaccccc gagacgagga tcgattcgcg tcgctgcgtc gcaggcacgc
      421 gcgaacgcct tgaagatgca ctcgatcatg tggtgcgcgt tgccgccgta atgaacgttg
      481 acgtgcaggt tgcactgcgc acgggtcgcg aaggccgcga agaactcttt cgccagctcg
      541 cagtcgaacc cgccgatcca cttgccttcc aggccctgga ccttccacac gaacgcggga
      601 cggccacaga aatcgatcgc gaccgtgacc atgacctcgt ccatcggcag ggtcgcccag
      661 ccgtagcgca cgagcccggc ccggtcaccg agcgcctgct cgaaggcatt gccgagcacc
      721 aggccgatgt cttcgacggt gtggtgcccg tcgatctcga tatcgccagc cgcggtaacc
      781 gccaggtcga acacgccatg ccgggcaaat gcgtcgagca tgtggctcaa gaacggcaaa
      841 ggcgtctgga tcgtgcgctg gccggtcccg tcgatctcga ggtcgacgct gatctgcgtc
      901 tccttggtgt ttcggtcgag cttgacgcga cggcccacgg acgtacctct caaccttgta
      961 tcagccccct cgcgatcgta caacgacggc atgcgtggct acgtcctcgt ggtgggttgc
     1021 acgatctcgc tctttgcatg cggtggcggc ggagagaaga aggtcaaaaa gccgaagccg
     1081 aagaaagagg tcgaagagac cgtaaaggcg gagacgcccg aagatcgcga gaaggcgcga
     1141 cacgaccaag cggtcaagat cgtcccggaa ggctcgacct gcctgccggc gtcgctcaaa
     1201 gaagacggtg cgcctcgtct cgagctggcc gcgtccggct ccgaagcgat cctctgcgcg
     1261 ctcgacgaag atcagaaccg gctgctgggt ccggtcggtt gctggaagat caaccttgcg
     1321 aacggagagc tcgagtacaa gggcacggac ttgccgccgg gtcgcggcgt ctgggtcaag
     1381 ctcgacgaga gctgcgccaa gggctactgc ctcaagagcg tcaagagcac gaaggcgctc
     1441 gtcgcaaagg atctcgacgg caagaaggtc gcggtcctcg cgggcgacga cgttcatctc
     1501 ttcgacgccg agtccaaaga agagtcctcg tcgttctcga ttcgtggcga taagggtctg
     1561 acgaacgatc cgaagggcat ggtcttcgtc ggcgatcacg tcgcagttga aggcgcggac
     1621 gagggcccgt actccgcggt gtgggtcttc aagaccgacg gtacgcagct cgggccggtc
     1681 acgatgctcg gcggcaaaga agagaaaccg gtctcgacgt acaagggctc tctgtcggtg
     1741 ctcgacaaga accgcatcgc ggtcgccgac cacggcatgg agacgctgac gacgtaccag
     1801 ctcgataacg gcgcgcgcgg caagctcgtc cgcaagctga aggcgaagcc ggcgtgcaag
     1861 ccggtcgagc tcgacgccta ctggcacgag ggtgacaagg tcaccgacaa gtgcaaagac
     1921 agcatcgaga agggctacgg cctcatgatg ggcgcgacgc tcgtcgccgg tgcgaagaac
     1981 aacctcgcac tgctgcgcgg cgatcgcctc ggtgacctcg gcgtgctcga cccgaagtcg
     2041 ctcgaagaga agaaggcgat caagcttccg tggtgcgcgg ccgatgccgc tggcgcaggt
     2101 ggtggcggtg gcggtggcgg tggcgacgcc gacgaggaat cgaaggacga ggcgaaagac
     2161 gcgaagaaag actcgaagga gtcggacgcc gcgccggccg acaagaagaa gggcaagtcg
     2221 aagcccggcg atacgtcaga tccgcaagaa ggcggccagt aagcacgcgc ccgcgtcggc
     2281 gcggacggtc gacgagcagc tcgcgtcgac gagcagttcg cgtcgacgag cagtcgcggt
     2341 cgcgtcgccg tgattagcgg cgcagcgctt ggaggcgttc gcgcgaggcg gccacttctt
     2401 cttgtggcat tgcgccgacg aggtcgaggg acgcgcgctg ctcgttgcca gtctgcgcgt
     2461 cgcgtgcgcg gacttggagg atgccgttcg cgtcgaggtg gaacgtcact tcgatcgacg
     2521 tttccccgcg cggtcgtcgc ggtagcccct gcagcagcaa gtcaccgatc acgacgttgt
     2581 tgtcgaggcg gcgcgactcg ccttggcaga cgatgatgcg aaccgcgtcc tggtgatcgc
     2641 gcgatgtcgt gaagagcttg cgcgtctccg tcggcacgcg cgagttgcgg cggatcagct
     2701 cctcgcagta gccggcgacc gtgccgatac cgaggccccg cggcgtgacg tccatgatga
     2761 cggggctgcc gccacccgcg cgtccgatgg gcgtcggcgg tgcagattcc ggcgacggcg
     2821 gcggccacac cggcggctgc agcggaccgg gcggcaagcc ggacggcaag ccagacggcg
     2881 cgatcatcgt cggcgattgc gacgcgggcg actgcgggat catcgtcggc gattgcgatg
     2941 cgggcgaagg ctcgagcatc agtgtccgcg ctgcgggcga tgaggtcggc gacggcggcg
     3001 gtgatccaaa cgcaccgacc ggaggagtcg gcggcagcgg tgctgcgccg ggacgagcgg
     3061 gcggagcggg cggcgcaaat gcatcggggc gaggcgacgt agcggtcgac gacgccgagc
     3121 caaggccacg cgcggtggat gcgggagacg tcgaatcgtc gagatcgagg aagcccttct
     3181 cggtgcccgt gtcgggaaaa tcgatgtcct cgctcgtcgg cgcgaccgcc gcggggccgc
     3241 gtgggcctgg acgagtcgca gcacccgtcg ctgcgccaaa gccacgcgcc gtggatgccg
     3301 gcgacgtcga gtcatcgaga tcgaggaagc ccttttcggt gcccgtgtcc ggaaaatcga
     3361 tgttctcgct cgtctgcagc gtcggctccg aagccgcggg atcgacgatc accgtcgacg
     3421 attcgcgctc tggaacgacg agcgtcttgg cttgcggcga cgacggcggg gccggcggcc
     3481 tgacgatcgg catcgcgatc gctgttttgc gcgatggctt gccggggggc accggcggcg
     3541 gtagcggttg cgtcgtgccg tgcggcggag tctgcgcgac accgcgtgcg gtcgtgcgcg
     3601 gcgcggcttg cgcgacaccg ggtgcagtct ttgcgcccgg cggcggtttg atctctttcg
     3661 tgaggcgacc gatgaccggt tggccggcgg gtggtgtggc tgcgggtccg gctttttcct
     3721 cgagtcgatc gtggtgaccg ccgcgcagcg agtacgcagt gtccgcgcga tcattgcgag
     3781 gggcggccga ttcgttcgtg cgctcttcgt cgccacggct gaacgtctgc gtctcgttgc
     3841 cgccgaacgt gctctcttct tgctcgaact ggccgaccgg cgacggcacg ccgctgactt
     3901 gcgagacgga cgacttcttg ttgaggacgc gctcgagcgc tttggcttgg aatgcagcgc
     3961 cgacggcgac agcatcctcg gggttgacgt ccgtgcgcgg tgccttgttg aagaacttgg
     4021 agacctgatc gcggacgtac ggaatcttcg tcgtgccgcc gacgaggatc acgtcgttga
     4081 tgccgtcgat cgtgaggttg gcgtacttca gcgcctcctc gcagactggg aacgtctggt
     4141 cgatgatgtc ggcgacctgt ttgacgaact cgtcgcggcg aatctcgatc tggagatcga
     4201 gcggcttgcc cttcgcgccg tatgcgattt cgtcgacgcg cacgacggca cgcgaacgcc
     4261 gcgatagctc gatcttcgtt tgctcggcga cggcgcgcag ccgcatcatc gagacttcgt
     4321 tcgtgcggag gtcgacgcga ttctcggcga ggaacttctc gaccattcga tcgacgaggc
     4381 gctcgtcgag atcgtcgccg ccgaggaacg tgttgcccgc ggtgcccagc acttcgtaga
     4441 cctgatcggc gagcttgagg atcgtgatgt cgaacgtgcc gccgccgaag tcgtagaccg
     4501 cgatcgtctc gttgagattg cgcgcgttgc cgtatgcgag agcagcggcg gtgggctcgt
     4561 tgaggacgcg cacgacctgc agaccagcga tcgagcccgc ggtcgcggtt gcagagcgct
     4621 gcgcgtcgtt gaagctggcg ggcaccgtga cgaccgcgcg gcccacttcc gtcttgagct
     4681 gggtgctcgc gatcgtgcgc gcgtgatcga ggacgatcgc cgagatctct ggcacggcga
     4741 actcgccacc acgcgtgaca acgagcggca gctggttctg gccctccttg atctggtagg
     4801 gcatgcgctg cttcgccgtc tggacctccg gcgaattcca cgcgcgaccg atgaggcgct
     4861 tgaccgagaa gatcgtgttc ttcggatcga tgaccttgcg ctgcttggcg gcggcaccga
     4921 ccaccacgcc gccggtcgga tggaacgaca cgaccgacgg atggatcttg aagccgttca
     4981 gatccgcgag gacctgcacc tgtcccgttg tgtcggcatg ggcaaccacc gtgttggagg
     5041 tgcccaggtc gattcctaca atcgggtcgc tcattacaac gcgctcacgc cctctcgaag
     5101 accaagccta tcgccggtct tccaaggtgg cgaggatttg gtcgtaccag cgctcgaggc
     5161 cccggcggaa gggccacatc cagccgtagg agggactcgt gaactgttcg atcacgtagt
     5221 tgcggtggtg agaaccccgg acgtacccga ggccgctcac ctcggctcgc gctcgaatgg
     5281 tctcggcgta ggcctccttc tcgaaccgcg ctcggaagta ggcgacgccc atcggcagcg
     5341 gaacgaagat gtagagcacc gccatgaccg gaagcgtgag cttgcgaaat tgccggaggt
     5401 gcaccgcttc atggcgcagc gtcacgtacc gatcgtccgg ctcccaatcg tcccagtccg
     5461 cggtgacgta gaccgtcttg ccgatcgtcg tctggaacga gtcgaggtag gtcttcatcc
     5521 ggccgaacgt gacgaccttg agcgcgacgt cgatcgcgcg atgcagcgcc gactgatcct
     5581 tccgaacgat ccgaaatccg ggaatctctt cacgcagcgc ttcgacgagc acgcggtagc
     5641 gatccacgtc ggcatcgtat ccggagtcag ttgacggatc gagcccagaa cattagcgtc
     5701 gacggctcgg tgtacgtcga tctgtccaag ctcaatccgc ctcagaggga agcggcgagc
     5761 cacggcgggg ggccgatctt ggttctcgcc ggagccggtt cgggcaagac gcgcgtcatc
     5821 acatttcgga tcgcgcacct actcgggcaa ggcgtctcgc cgcgtgcgat ctgtgcagtg
     5881 acgttcacga acaaggccgc cgaggagatg cgagagcgca tcgcggcgct gctcggcgat
     5941 cgcgagctcg cacaacagct gacgatcggt acgttccacg cgctcggcct gcagatcctg
     6001 cgcatggagc gcaaggcgct cggcctgccg cgcgggttcg cgatctatga ccaatcggat
     6061 cagctcggcg ccgtccgcga agcgatgcgc cacattcacg acggcgatcg tcgcttcgac
     6121 gcgaaggcgg tgctgacgcg gatctcgctc gcgaagaacg cgtttgtcgc gcccgaagag
     6181 tacgaaggca atcccgctga cgattacgac gcgatgaccg cagaggtcta tccgaagtat
     6241 caagagacgc tgcggtcatg cgcggccttc gacttcgacg atctgatcgt cgagccagtg
     6301 cgcctctggc agcgcgacgc cgaagtgcgc agcaagtggg caagccgctt tcagtacgtg
     6361 atggtcgacg agttccagga cacgaacaaa gcgcagctgc gcatggtgaa gggcctcgtc
     6421 gagcaacacg gcaacctgtg cgtcgtcggt gatgacgacc agtcgatcta cagttggcgc
     6481 ggcgcggacc cgacgaacat tttgcagttc gacaagatgt tcgagggcgc gaaggtcgtg
     6541 aagctcgagc agaactatcg ctcgacgaag acgatccttg ccgcggcgaa cgaggtcatc
     6601 ggcaacaaca aggatcgcca tggcaagacg ctgtggtccg atctaggcgg cggcgagatc
     6661 atcacgcacg cagttgcacc gaccgccgaa gacgaagcga agtggatcgc gcgcgagatt
     6721 cgccaactcg tcaaagaggg ccggcgctgg ggcgatttcg cggtgctcta ccgctcgaac
     6781 atccaggcga aggcgatcga agaagagctg cgcacggcgg aagttccgta cgtgatgtac
     6841 ggcggccagc agttcttcga gcgcaaagaa gtgaaggacg tcatcgcgta catgcgcgtg
     6901 gcgctcaact cgcgcgacga gctcgcactg cgccgcgtga tcaattatcc ggcgcgcggc
     6961 atcggtgcga cgacgatcga caagctcgtc cagcacgcgc acgcgacgca cacgacgctg
     7021 tgggaggcgc tctcgaatgc ggaagaagcg ggcgtccacg gcgcggcgcg caccgcgatc
     7081 gaagagctcc aacacatcgt gcgcacgatc ggcgcagcgt ttgacgccgg cgacaacatc
     7141 gtcgaagcga cgaagcagat gatcgaggac atcgggctct acgacgatct gcgctctgcc
     7201 gccggcagca tgtcggccgc gcaacgccgc atcgacaacg tcaccggcct gctcaactcc
     7261 ctcgagcgct gggccgcgaa gggcaaaggc caaaaggagc tcgccgagta cctgcgaatg
     7321 ctctccctcg actcgaagga cgagagcgag aagcatcagg acaacggcga gaaggtcgtg
     7381 ctgacgacgc tccacggcgc gaagggactc gagttcccgg tctgcttcat gatcggtctc
     7441 gaggaagagc tactgcctca catccgcacg ctgcagccgc aggtgacgga tccgagcgac
     7501 gccgatcacg cgacggatat cagcgaggag cgccggctct gctacgtcgg gatcacacgt
     7561 gcgcagcgca agctgtacct gactcgtgcc tgcacgcgcg tcagccgagg ccggatcctg
     7621 cagcgcacgc cgtcgcgatt cctcctcgag atccccgacg agctgctcga gattcgcgac
     7681 ctcggcgaag aggcgcgcca agccgttccc aaagaggagc tgaagacctt cttctcgtct
     7741 ttctcgttcg atgactgatt gactgagaca tcgctgtcgc agggtgtcct gtgacctgtg
     7801 gtctctaccg tgtcagcggg agtcggcggg ggacccagcg tcctcgtcgg cgatcccgtg
     7861 gtatttgccg aggcgatgaa gcgcaagcgc aatgccatcc tgtgcggagc cctgctcgct
     7921 gcctgcggcg gttctcccaa gaccgcgccc gtcgagctcg gcacgacgca ttcgtcggga
     7981 gacgtcgtgc ccgcgaagcc cgctgtcgtc gaggcggtgg atccgaatct cgcgttccgg
     8041 ctttcgtact cgaacccggg cggcatgtgg atgcccgcgc agatgacgct gccgcagcat
     8101 gtcgagacct tccgcaagat gggcgtgaag ctgcccgccg atgcgctcgc gaatccgctc
     8161 agcgagacgc tgacgtcgat cgtgtcgctc ggcggctgca gcgcatcgtt cgtatcgccc
     8221 gacggtctcg tcgtgacgaa ccatcactgc gtgcaaggcg cgctgaagt
//
LOCUS       DATLBZ010000140        17532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1627617, whole genome shotgun sequence.
ACCESSION   DATLBZ010000140 DATLBZ010000000
VERSION     DATLBZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17532
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627617"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..150
                     /locus_tag="VMZ53_07535"
     tRNA            51..150
                     /locus_tag="VMZ53_07535"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:86..88,aa:Sec,seq:tca)
     gene            281..2272
                     /locus_tag="VMZ53_07540"
     CDS             281..2272
                     /locus_tag="VMZ53_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28343.1"
                     /translation="MTTVAYAETSSHYEETVRYETATSFGYEEASVKVEYQFITCFGN
                     FVLTYRLVPDSLTVGEWYRFDGERELVGDEAPPRVSSVEFAGTVRGIRGDDIKDFSDG
                     TAHSKTGMGCYGQTVTIGRITDITGNPKPTKDDIARALGTFSIRVRRAQGSIRNSAID
                     DRMRNKRLEKKRADEQAERDAKRKADEAKRIKDAQDKKDEQDRKDAEAKNGPPDKTGE
                     KTDGPNPSDQEVKKDGDGQPSVDAQVAANLENNRKMREEAERKAREDAERRKPAPEAT
                     PPPQDDSWKPRHKKRTEPNEKSKRALEWLERHTGRTELHGYYCSMLGMRHVGCDADSY
                     VPTIDEYGIDGSGNEVAIWNQVQRFNVTDMGRIMVIYGPLSLAPQGIWNVMYLTPLDA
                     SPEDYLMQLYDASDFDDARVLGFAPVRDRDAHEDEPDPDPTQPRFSPGAEAAAAAFTG
                     MEESLDRAGIFGEHHIAFVHDGLGASADMDGTPAPSRTGLTMTYCRVAGAQLGFGYAS
                     TGKSTSTATDFEYRTAGTISFYDKGGYLTAGGGLCVPNRWVQPYALVSFELPLGGSRT
                     AVGMNELRIAAGEGDTIGGRFVPTVGVNVRIPKIRIGLGLRASYTKVLPRNTLVDATD
                     GTGSTWSAEGGGAGYFSLGIQVLVYPFRSGTPSPYTKLD"
     gene            complement(2273..4174)
                     /locus_tag="VMZ53_07545"
     CDS             complement(2273..4174)
                     /locus_tag="VMZ53_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HUS28344.1"
                     /translation="MTTNGDLHLGPDFAASLAKALPALLAPGALPDDWDHRATTYDLP
                     RFDSDVDFASRFEAALSDVVASSELRAPDQLRQRLAAVGLPFDYARLGQPLSTVYELY
                     MQSRTNAARCFSFASVTKPWLAVIESTTRSLPVRIYARATLPISDEKRRALQRGGCEL
                     HEQWRQDLPPRAADVLTVLVTESPFVGDIQTSAADAVCFAVSHGGVLLIRDAARISPY
                     GIQLIRKRTVAALLAADARAELERAAGLTPTTLAAATPAECEKKLRGLFPQIHDSLYF
                     CTGLAAEAAVFTAVADVLGPEPVTLFYAQNGYGGTGQLIADLLARGGAIRPAPLAVIA
                     QDTSVTLVDRVLAALEALGGAPAFVFLETPTNPELQVHDFAALMTGVRAYAQRWGTKV
                     PIIVDTTLAPLYPIFAKDFAQDWPFILVKSGSKYFTKGKATLGVVACAANPTALAIVA
                     RARALGQDADSFARPAQLAELSAGLEDLVPRMAAISAHTIRLASALRAALRAHGHEIT
                     LYAISKEQVADGLATGMLSFYLPPAPTTYPDLVDEFVAYLLEHAPTLVKSRVSYGQST
                     GGGKPDYFYVINPEESTQGALSAEVKNAQKHDNVQICRISVPEHADVDGLVAAMNGFF
                     AVKYARASK"
     gene            complement(4232..4861)
                     /locus_tag="VMZ53_07550"
     CDS             complement(4232..4861)
                     /locus_tag="VMZ53_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28345.1"
                     /translation="MVSRIVHHGDGLEYLRSGPLPSDHALVTSLPDHSELRGLGVDGW
                     RRWFVDTVELACRAVADDAVAIFYQSDVKHEGRWIDKGHLVMCGADAAGSSLLWHKIV
                     CRAPAGTETYGRPSYAHMLCVSRTLRLDAGYRAVDVLPQLGEMTWARAMGSAACDAAA
                     VFIAEHTRCRVVVDPFCGLGSMLTAANARGLDAIGIELDPKRATRAERR"
     gene            complement(4872..5756)
                     /locus_tag="VMZ53_07555"
     CDS             complement(4872..5756)
                     /locus_tag="VMZ53_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUS28346.1"
                     /translation="MTRRARDTFTGTLRPNDPRRFLVEAMLGAMGADGKIHEAELASM
                     QRHLEEHEMFSGLSDRNHAVLLQIARDSISFAKDPVARIPAIAKGLPSRLHKLTALAM
                     ACEVVVADAVIDPAEELYLETLRGALRIAAPDFAEMFRAAREQRSVRDLDARVAHLRE
                     LVPLIVELFALQSLSLGVLTPAHRGQILELLTALPDLALRDHELATLVERAYARMHFS
                     MDLEVELQKVATAMPNPIDRYWAIIYLMCADTQRAPHWRSSPFLPLLQRHFALREEYF
                     DLAATDAAGFASILPQAS"
     gene            complement(5810..6649)
                     /gene="ylqF"
                     /locus_tag="VMZ53_07560"
     CDS             complement(5810..6649)
                     /gene="ylqF"
                     /locus_tag="VMZ53_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HUS28347.1"
                     /translation="MSLQWYPGHMTKARRELAELMPSQDVMIEVLDGRLPAASSNPLL
                     AEIRGSKPVIKVLTKSDLADPAITRAWLDYFEAEPNVKAFAASVDRAQLTRKRIAELC
                     QPFAHGRGPDKPVRALIAGVPNVGKSTLLNTLMNRAVAKVGDKPAVTKEQQIVHLPTG
                     NIVVTDTPGLMWPKIEHEARAFRLAFAGSIPDTAIDYLTIGMFGASYLLERYPDLVIA
                     RYKLAAKPATADALLHEIGRRRGGLRKGGTVDLHKAAELFVHEFRGGTLGPISLEAPP
                     VKD"
     gene            complement(6670..8367)
                     /locus_tag="VMZ53_07565"
     CDS             complement(6670..8367)
                     /locus_tag="VMZ53_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUS28348.1"
                     /translation="MARLVYITAQGWQAIELQPHQGVGRHPNNAVQLLDTIVSKEHCV
                     IERRGPSMVLRDLGSMNGTYINGERVAGSRVLRHGDEIRLGNTALQFDDGATPFHFPP
                     PPPSPGVIHHPFVANFDPATGAPLTGGTPQWMVKRRTAEAVEVPAHDHLVSVDNSKHS
                     IGQQVAVQSKEFLPFEDAVRDTAALRLDYERLRITWELLREIGLERDFDTLLSKILVA
                     LFRFVAADRGVILIKEPDGTLTPRAHRRRDGATAPINISSTILAHVTKERAGVMTHDA
                     GSDFGGKSMFINRITSAIVVPLLHDKEVIGALWLDSEKIASFKPKDLELLTAVGNQVA
                     MLISNTMLGLKVQQEIMTRDRFSRLLSPNVAEQVISGKLDIKKGGIHVPMATVFNSDL
                     RGFTRMSEAIPAEEMVELLNEYFELMVECIFKYEGTLDKFMGDGIMAFWGAPAFHADD
                     AVRSVQCSLEQLAVLDELNVHRKSLGAVPLAAGIGIHSGPLISGYVGSSKSLSYTVIG
                     DTVNTSARLCGIAAGGQILVSEETANQLGGRFPLQQLPDATLKGKEKPLKIFEVRRT"
     gene            complement(8394..8972)
                     /locus_tag="VMZ53_07570"
     CDS             complement(8394..8972)
                     /locus_tag="VMZ53_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28349.1"
                     /translation="MRTILLLAAAAAACKGSDKAPSPSGADKPKAKAEGPERISNHEL
                     ASTPLQNVASTAGTVAFTIDLPSAALKPPEVKGAYSTWEPKKAWFDTPSFTVLFSEMP
                     MSPDATGDSQPLGDDAKDRTIARAEKLPDGGYINVDQRTDHAFFKLEVCRPVNNGSLC
                     CSVIQRADKPIDSFDESLKMAETICTSMKATR"
     gene            9174..10388
                     /locus_tag="VMZ53_07575"
     CDS             9174..10388
                     /locus_tag="VMZ53_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS28350.1"
                     /translation="MIPIAVAYLAVFVVAAIVWRSVALGVGSAAIVVYLAALGGARVS
                     LARGEVERAALLTGGGLLAMIGIAVFAVHFQYPAFALIAIAAVVVVLPHVERRVLWWF
                     AGTAIAAVVIVTTVGALPPAFEQPPLWFREIVLLSSLFAASCLIILMLAADHRRMRGL
                     VRESTELAGVAQRQRDATDAFLIAASHQFRTPVSTLLLQSESLLRGAPDDASKRRLER
                     LMRSADRLHLLVDTMLDVSRITSGSLQLERTVVDVAALVRAVAATQQAGHASDARVEV
                     LIVDDGGESAEELHVEADARRLELMTSHLIENAVRLGKGAPARIEVRRDARDVLIAIT
                     DGGKLLDVSTRERIFDREALQSPQAEHDGGPAVGLWLVRELSRAMGGEVSANEGANGG
                     TRFEVRLPRRVV"
     gene            10391..10747
                     /locus_tag="VMZ53_07580"
     CDS             10391..10747
                     /locus_tag="VMZ53_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS28351.1"
                     /translation="MVLVVDDDSDLRETICDVLEAYGHRTHAARDGEEALAVLRREAG
                     IEIVLLDLMMPIMNGWEFRASQLADRELARIPVVVMTAAADLTRSPIAAAQVLPKPVT
                     TKALLDAIAQHALAPQ"
     gene            11311..12918
                     /locus_tag="VMZ53_07585"
     CDS             11311..12918
                     /locus_tag="VMZ53_07585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28352.1"
                     /translation="MGERSSSRGDTRAELEPLSADSRAFLQERLTLFGRWFALVAIAH
                     YFIANTPTDLFLGYPFDWISEYFGPQEQLELAVTACAALVWVVARGTSRSVRILRVID
                     AAAIVVPMALLALLVHHATAAHGDLGEIAGVLLACTNVVVVRAVVVPSTARRTLVVSA
                     VAWLPVVAVTVYALATSDGATRSDINMALATVMWAVVSTACAAIASRIVFRLRVEVAE
                     AEALRLGQYTLQQKIGQGAMGVVYRAHHALLRRPTAIKMLAPDRNSATDLQRFENEVQ
                     HTARLTHPNTIAIYDFGRSREGTFYCAMELVDGPSLEEVVRQDGALPAWRVVHLLRQI
                     CGALAEAHAMGIIHRDIKPANLMLCERGGQLDTIKVLDFGLVRNLRDLETEHRVVGTP
                     LYMAPEAFENPDGVDERCDLYSLGATAYFLLTGTPPFSGATTVAICANHLAQVPEPPS
                     RRLGGPIAEDLERLVLRCLAKAPSDRPRARELGELLSECVDASGWSNERVAEWWRAHE
                     RPATGDSDRMNATGTLTVEPIATRDAS"
     gene            complement(12915..14270)
                     /locus_tag="VMZ53_07590"
     CDS             complement(12915..14270)
                     /locus_tag="VMZ53_07590"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS28353.1"
                     /translation="MDVGELARVAEVVLGNPFETVAGREEAHAAVQAARARGTATLED
                     LARKGQHKVARRLLVAFVVDELAHEAVASFLDPRQCPERIFSELRLSREAVREVTASA
                     ARCAASLHHIAGTSQAIARVRAAVWRACHGESVYDAISMRPLIREQNVLVLGETGTGK
                     ELVAQAIAASDPDPDRVQVLNAAAIPEPLLESELFGHKKGAFSGSVGDREGKISAADG
                     GTLFLDELADLPPVLQAKLLRVIDDDVVTPLGANQGKKVDVRYVSATSQPLHAMVERG
                     AFRRDLFERLAGITIVLPPLRERLEDLRPIAERMFEQQSAPMARSGASTANVGRLIVH
                     QARFLAWLDSDEARARPWNGNVRELQTTLRRWLLGFRETPATTVEPTADATKAQSQPQ
                     GLARVVDGAATLREVEDWYILHVLDKVEQHQRRAADVLGIDRGTLARRLRQIDGTDDE
                     R"
     gene            14418..15680
                     /locus_tag="VMZ53_07595"
     CDS             14418..15680
                     /locus_tag="VMZ53_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28354.1"
                     /translation="MSFATRAILVGGLLAAIAVLATCQHLPLLTWTPQLVEPRTYPLL
                     FGQLFVAWLALAVSPRIWRRPILVIASLASLVTLSPLFAVVAVVHAGLTTAVLRAPLP
                     RIVVLAFLVGSVAAAVAVGNAELGDEARRLAIVFALGQTFRLFALYHAARVAGARPLS
                     LVDTFLYLHLLPCNVIVPYMLCIPRVERFRKSLDTPRAFDVRLLAAGAVVGVLVHAGL
                     RVFDPKIALLHAAQAHDIPRVAAFALLFYPLSGVLGILGPAWILTGMMRLVGIDVDPP
                     FDHPLSSTSFLEWWRRWNTHFRDLLVDLFFFPVVLGRLRRRRRIATVIGAAAVFLVGS
                     TLFHMPKHMLLVAHGWEMPWGLFAENVVSFVFVTVGLLQGARRRPPRRRLTAIFVTWI
                     VVFVAVHIGYLVGWSLHPDRLPKESSKL"
     gene            15677..15913
                     /locus_tag="VMZ53_07600"
     CDS             15677..15913
                     /locus_tag="VMZ53_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HUS28355.1"
                     /translation="MSIDSRPALRAFISDLAVGAMFTDDVDLVKEGIVSSLQLMELIA
                     FLEDRFGVVVTAVDLHNARLASVATMAALIEEFS"
     gene            15916..17367
                     /locus_tag="VMZ53_07605"
     CDS             15916..17367
                     /locus_tag="VMZ53_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HUS28356.1"
                     /translation="MDIFAMIDAHAARAPSALVFISSAGRLTWGELDARANALAGHLH
                     RTLVRGSAVVVRGQKEPGMLIALLACARAGMAFVPIDEALPMARQALIVRRSRAPLIL
                     EAAALPAVENSEPAPAAARLTRDDIVYIMFTSGSSGEPKGVPITFGNLQGFLDWACEV
                     HPIRGGKRFLDQAPYSFDLSVLDTWGALVTGGSLFAVDRATVANPLLLAPALAAGGIE
                     VWVSTPSFAELCLGDRRFDAAILPRLSCFLFCGEPLTPATALRVKQRFPAARIYNLYG
                     PTEATVAVTSALIADHHLSRDAPLPLGTPLPGTRVFVANDAGDPVPDGESGELVIEGP
                     CVSPGYLDAPELTARSFSGHAPRRHYRTGDAGRVVDGELRFEGRLDGQIKLFGHRIEL
                     DDIAANLTALPNVARGVVIAVEEGGRAVALVGFVVGAPGARLDPRQLRDELATRVPSY
                     MVPREIRVVDALPMTVNGKLDRRSLAELSRQRP"
BASE COUNT         2809 a         5530 c         6013 g         3180 t
ORIGIN      
        1 ttcctagcgc atcgcttgac gaccagaaat ggcagtgtag tttcgccggt ggaagcgcgc
       61 gttggactgg tgccagcctc ggtcttcaaa accgatgatg ctctgcgcga gcagcggcat
      121 ggtgggttcg attcccatgc gcttccgcca tcacgagcgt gatctcgtgg ccgtgcgcgc
      181 ggcaggaccc gatccttacc cggcacgggt tgcgggcgag gcgcgcgtcc ccgtaaattg
      241 cgggacagtg cttcgcgctg tacttgttgt tgttgttctc ttgacgaccg ttgcctacgc
      301 cgagacgtct tcccactacg aggagacagt cagatatgag accgcaacgt cgttcggcta
      361 cgaagaggca tcggtcaaag tcgagtacca gttcatcacg tgcttcggca acttcgtact
      421 cacgtatcgt ctggtccctg actcgctgac cgtcggggaa tggtaccgat tcgatggcga
      481 acgcgagctc gtcggtgacg aagctccgcc gagggtgtcg tccgtcgagt tcgcggggac
      541 cgttcgcggc atccgcggtg acgacatcaa agatttttcc gacggtacgg cgcactctaa
      601 gacggggatg ggctgctacg gccagaccgt gacaatcgga cgtattactg acatcaccgg
      661 aaacccgaag ccgacaaaag acgacatcgc gcgcgcgctc ggcacattct cgatccgcgt
      721 caggcgtgcg caaggctcga tccgcaactc ggcgatcgac gaccgaatgc gcaacaagag
      781 gctcgaaaag aagcgagccg acgagcaggc ggaacgcgac gcgaagcgga aagccgacga
      841 agcaaagcgc atcaaagacg cgcaggacaa gaaagacgag caggaccgga aggacgcgga
      901 ggcgaagaac ggcccgcctg acaagacagg cgaaaagacc gacgggccga acccaagcga
      961 ccaggaggtc aaaaaggacg gcgacggtca gccgtccgtc gacgcgcaag tcgcggcgaa
     1021 cctcgagaac aatcgcaaga tgcgcgagga ggcagagcgc aaggcacgcg aagacgccga
     1081 gcgccgcaag ccagcgccag aagcgacgcc gccaccacag gatgactcgt ggaaaccgcg
     1141 ccacaagaag cgcaccgagc cgaacgagaa atcaaaacgt gcgctcgaat ggctcgagag
     1201 acacacggga cggaccgagc tccatggcta ttactgctcg atgctcggaa tgcggcatgt
     1261 tggctgtgac gccgacagct atgtgccgac gatcgacgag tacggcatcg acggttcggg
     1321 caacgaggtc gcgatctgga accaggtgca gcggttcaac gtcacggata tgggccgtat
     1381 catggtgatc tacggcccgc tgagcctcgc cccgcaggga atctggaacg tcatgtacct
     1441 gacgccgctc gatgcgtcgc ccgaggacta cttgatgcag ctctacgacg cgagcgactt
     1501 tgatgatgcg cgcgtgctcg gatttgcgcc ggtacgcgac cgcgatgccc acgaggacga
     1561 gccggatccc gacccgactc aaccgaggtt ctcgcccggt gcagaggccg ccgcagccgc
     1621 gttcacgggg atggaggaat ccctcgatcg cgcgggcatc ttcggcgagc accacatcgc
     1681 attcgtacat gacggactcg gtgcgtcagc ggacatggac ggcacgccag cgccctcacg
     1741 cacgggcctg acgatgacgt actgccgcgt ggccggcgct cagctcggat tcggctatgc
     1801 ctcgacggga aagagcacga gcaccgcgac agacttcgag taccgcacgg ccggcaccat
     1861 cagcttctac gacaagggcg ggtacctgac ggccggcggc gggctgtgcg tgccgaaccg
     1921 ctgggtccag ccgtacgcgt tggtgtcgtt cgaactgccg ctcggcggtt cgcgtaccgc
     1981 agtcggaatg aacgagctca ggatcgccgc gggcgaaggt gacacgatcg gtggtcgctt
     2041 tgtcccgacg gtcggcgtga acgtccggat ccccaagatc cggatcgggc tcggacttcg
     2101 cgcttcgtat acgaaggtgt tgccgcgcaa tacgctcgtg gacgcgacag acgggacggg
     2161 gagcacgtgg agcgctgagg gcggcggcgc gggatacttt agtctcggaa ttcaggtcct
     2221 cgtgtatccg tttcggtccg ggactccatc gccctacaca aagttggact agctacttcg
     2281 aagcgcgcgc gtacttcacg gcgaagaagc cattcatcgc ggcaacaagg ccgtcgacgt
     2341 cggcgtgctc tgggacgctg atccggcaga tctgcacgtt gtcgtgcttc tgcgcattct
     2401 tcacctcggc cgagagcgcg ccttgcgtcg actcctccgg gttgatgacg tagaagtagt
     2461 cgggcttgcc gccaccggtc gactggccat agctgacgcg gctcttgacc agcgtcgggg
     2521 catgctcgag caggtacgcg acgaactcgt cgacgagatc tgggtacgtt gtcggcgccg
     2581 gcggcaggta gaacgagagc attcctgtcg caagaccgtc ggccacttgc tccttcgaga
     2641 tcgcgtacag cgtgatctcg tggccgtgcg cacggagtgc agctcgcaga gcgctcgcga
     2701 ggcggatcgt gtgtgcgctg atcgccgcca tgcgcggcac gagatcctcg agacctgcac
     2761 tgagctcggc gagctgcgct ggccgcgcaa acgagtctgc gtcctggccg agcgcgcggg
     2821 cacgcgcgac gattgcgaga gctgtcgggt tggctgcgca ggcaacgacg ccgagcgtgg
     2881 ccttgccctt cgtgaagtac ttcgagccgc tcttcacgag gatgaagggc caatcctgcg
     2941 cgaagtcctt ggcgaagatc gggtagagag gcgcgagcgt cgtatcgacg atgatcggga
     3001 ccttggtgcc ccagcgctgc gcgtacgcac gcacgccggt catcagcgca gcgaagtcgt
     3061 gcacctgcag ctcggggttt gtcggcgtct cgaggaatac gaacgctggg gcgccgccga
     3121 gtgcttcgag cgcagcgagc acgcgatcga cgagggtgac gctcgtgtct tgggcgatga
     3181 cggcgagcgg tgcggggcga atggcaccgc cgcgggcgag cagatcggcg atgagctggc
     3241 cggtaccgcc gtagccgttc tgcgcgtaga agagcgtgac cggctcgggt ccgagcacgt
     3301 cggccactgc ggtgaacacc gcggcctccg cggcgagccc ggtgcagaaa tacaagctgt
     3361 cgtggatttg tgggaacaag ccccgcagct tcttctcgca ctccgcgggt gtcgctgcgg
     3421 ccagcgtggt tggcgtgagg ccggcggcgc gctcgagctc ggcacgtgca tcggcggcga
     3481 ggagcgcggc caccgtgcgc ttgcggatca gctggatgcc gtagggggag atgcgcgccg
     3541 catcgcggat gaggagtact ccgccgtggg aaactgcgaa gcatacggcg tccgcggcgc
     3601 tcgtctggat gtcgcccaca aatggtgact cggtgacgag caccgtgagt acgtccgccg
     3661 cgcgcggcgg caggtcctgc cgccactgct cgtgcaactc gcagccgccg cgttgcagcg
     3721 cgcgccgctt ctcgtcggag atgggcaatg tcgcgcgtgc gtagatgcgg accggtaggc
     3781 tccgggtcgt cgactcgatc acggcgagcc agggcttggt gaccgatgcg aacgagaagc
     3841 agcgtgcggc attcgtgcgc gactgcatgt agagctcgta gaccgtcgag agcggctggc
     3901 cgagccgcgc gtagtcgaaa ggcaggccga ccgcggcgag ccgttggcgg agctgatccg
     3961 gcgcgcggag ctcggaggag gcaacgacat cggagagagc ggcctcgaag cggctcgcaa
     4021 agtcgacgtc ggagtcaaaa cgcggcaggt catacgtcgt cgcgcgatga tcccaatcgt
     4081 cgggcagcgc gccgggcgcg agcagtgccg gcaaggcctt ggcgagactc gccgcgaagt
     4141 ccggtccgag gtgcaaatcg ccgttcgtcg tcatcgctgg agaatagcgt tagggcggca
     4201 tctcgacatc cattcaccgt atcaccggcg actaccggcg ttcggcgcgg gtcgcgcgct
     4261 ttgggtcgag ctcgatgccg atcgcgtcga ggccgcgtgc gttggcggct gtcagcatgc
     4321 ttcccagccc gcagaacgga tcgacgacca cacgacaccg cgtgtgctct gcgatgaaca
     4381 ccgccgcggc gtcgcaagcc gcactgccca tcgcacgtgc ccacgtcatc tcgccgagct
     4441 gcggcagcac gtcgacagcg cggtagcccg cgtcgagccg cagcgttcga gagacgcaga
     4501 gcatgtgcgc gtagctcggc cggccgtatg tctcggtccc tgcgggcgca cggcacacga
     4561 tcttgtgcca gaggagtgag ctgccggctg cgtctgctcc gcacatcacg aggtggcctt
     4621 tgtcgatcca tcggccttcg tgcttgacgt ctgactgata gaagatcgcg accgcgtcgt
     4681 cggcgaccgc gcgacatgcg agctcgaccg tgtccacgaa ccagcgccgc catccgtcga
     4741 caccgagccc gcgcagctcc gagtggtctg gcagcgacgt cacgagcgcg tggtcggacg
     4801 gcagcggacc gctccgcaaa tactcgagcc cgtcgccgtg atgaacgatc cgcgacacca
     4861 tttggtagcg gttagcttgc ctgcggcagg atcgatgcga agcccgcggc gtcggtggcg
     4921 gcgagatcga agtattcctc gcggagcgcg aagtgacgct gtagcagcgg caggaatggg
     4981 ctcgagcgcc agtgaggcgc gcgctgcgtg tcggcgcaca tcagatagat gatcgcccag
     5041 tagcggtcga tcgggtttgg catcgcggtc gcgacctttt ggagctcgac ctcgagatcc
     5101 atcgagaagt gcatccgtgc gtaggcacgt tctacgagag ttgcgagctc gtgatcgcga
     5161 agcgcgaggt cggggagcgc ggtgagtagc tcgagaattt ggcctcgatg agcgggtgtg
     5221 aggacgccga gtgagagcga ctggagcgcg aacagctcga cgatgagggg gacgagctcg
     5281 cgaaggtggg ctacgcgggc gtcgaggtcg cgcacgctgc gttgttcacg ggcggcgcga
     5341 aacatttcgg cgaagtcggg cgctgcgatg cgtagcgcgc cgcgcaacgt ctcgagatag
     5401 agctcttcag ccgggtcgat caccgcgtcg gcgacgacga cctcgcacgc catcgcgagc
     5461 gcggtgagct tgtggagccg gcttggcaag cccttcgcga ttgctgggat acgcgcgact
     5521 gggtccttcg cgaacgagat cgagtcgcgg gcgatttgca gaagcaccgc gtgattgcgg
     5581 tcggagaggc cggagaacat ctcgtgctct tcgaggtgcc gctgcatgga ggcgagctcg
     5641 gcttcgtgaa tcttgccgtc ggcgcccatc gcgcccagca tcgcctcgac caggaaccgc
     5701 cttggatcgt tggggcgtag cgtgccggtg aacgtgtcgc gcgcgcgtct tgtcattgct
     5761 tcgagaacct tgcttgcgac ggtgcaatag caacgatgtc cagcgatggt caatctttga
     5821 cgggaggggc ttcgaggctg atcgggccga gcgtgccgcc gcggaactcg tgcacgaaca
     5881 gctcggctgc cttgtggagg tcgaccgtgc cgcccttgcg aagtccgccg cggcggcgac
     5941 cgatttcgtg gaggagtgcg tcggccgtgg ccggtttggc ggcgagcttg tatcgggcga
     6001 tgacgaggtc ggggtagcgc tcgaggaggt agctcgcgcc gaacatgccg atcgtgaggt
     6061 agtcgatggc ggtgtcgggg atcgagccag cgaaggcgag gcggaatgcg cgcgcttcgt
     6121 gctcgatctt gggccacatc agcccgggag tgtcggtgac gacgatgttg ccggtcggga
     6181 ggtggacgat ctgctgctct ttggtcacgg ctggcttgtc gccgactttg gcgacggcgc
     6241 gattcatcag cgtgttgagc agcgtcgact tgccgacgtt ggggacacct gcgatcagcg
     6301 cgcgcacggg tttgtcgggg ccgcggccgt gcgcgaacgg ttggcagagc tccgcgatcc
     6361 gtttgcgcgt gagttgcgcg cggtcgacgc tggccgcgaa cgctttgacg tttggctcgg
     6421 cctcgaagta gtcgagccat gcgcgcgtga tcgcggggtc ggcgaggtcg cttttggtga
     6481 gcaccttgat gaccggcttg gagccgcgga tttcggcaag cagcgggttc gagctggctg
     6541 cggggagccg gccgtcgagc acttcgatca tcacgtcctg cgacggcatc agctcggcga
     6601 gctcgcgccg tgccttcgtc atgtggcccg ggtaccactg cagcgacatg cccgctgtat
     6661 gccacggttc taggttcgac gaacttcgaa gatcttgagc ggcttctctt tgcccttcag
     6721 tgtcgcgtcg ggcagctgct ggagcgggaa tcgaccgccg agctggtttg ccgtctcctc
     6781 ggacacgagg atctggccgc cggccgcgat gccgcatagg cgagcgctcg tgtttaccgt
     6841 gtcgccgatg acggtgtacg agagcgactt cgagctcccg acgtagcccg agatcagggg
     6901 accggagtgg atgccgattc ctgccgcgag cgggaccgcg ccgagcgatt tgcgatgcac
     6961 gttgagctcg tcgagcactg ctagctgttc gagcgaacat tggacggagc ggaccgcgtc
     7021 gtcggcgtga aatgccggcg cgccccaaaa cgccatgatc ccgtcgccca tgaacttatc
     7081 tagcgtgcct tcgtacttga agatgcactc gaccatcagc tcgaagtact cgttcaacag
     7141 ctcgaccatc tcctcggcgg ggattgcttc actcatgcgc gtgaacccgc gaaggtcgct
     7201 gttgaagacg gtggccatcg gaacgtggat gccgcctttc ttgatgtcga gctttccgga
     7261 gatgacctgc tcggcgacgt tgggcgagag gaggcgcgag aatcgatcgc gcgtcatgat
     7321 ctcctgctgg accttgaggc cgagcatcgt gttcgagatc agcatcgcca cctgattgcc
     7381 gacggcggtg agaagctcga gatctttggg cttgaacgag gcgatcttct cggagtcgag
     7441 ccacaaggcg ccgatgacct ccttgtcgtg aagcagcggg acgacgatcg cgctcgtgat
     7501 gcggttgatg aacatcgact tgccaccaaa gtcggagccg gcatcgtggg tcatgacgcc
     7561 tgcccgttcc ttcgtgacgt gcgcgaggat cgtggacgag atattgatcg gtgcggtcgc
     7621 accatcgcgg cggcggtggg cgcggggtgt cagcgttccg tcgggctcct tgatgaggat
     7681 gacgccgcga tccgctgcga cgaatcggaa cagcgcgacg aggatcttgg acaggagcgt
     7741 atcgaagtcg cgctcgaggc caatctcgcg cagaagctcc cacgtgatcc ggaggcgctc
     7801 gtagtccaga cgcagcgcgg cggtgtcgcg gacagcgtcc tcgaatggca ggaactcctt
     7861 gctctgtacg gcgacctgtt gaccgatcga gtgcttggag ttgtcgacgc tgaccaggtg
     7921 atcgtgcgca ggaacctcta cagcttcggc cgtacgccgc ttgaccatcc attgcggcgt
     7981 gccaccggtg agaggagccc cggttgcggg atcgaagttc gcgacgaacg ggtgatggat
     8041 gacgccgggc gacggcggcg gcggcgggaa gtggaacggc gtggccccgt cgtcgaactg
     8101 gagcgcggtg ttgcctaacc ggatctcgtc gccatgacgc agcacgcgcg agccggcgac
     8161 acgttcaccg ttgatgtagg tcccattcat gctgccgaga tcgcgcagca ccatcgacgg
     8221 cccgcggcgc tcgatgacgc aatgctcttt gctgacgatc gtgtcgagca actggaccgc
     8281 gttgttcgga tgccgcccga cgccctgatg cggctggagc tcgatcgcct gccagccctg
     8341 ggcggtgatg tagacgagac gagccatagt ccccgacagt atcgcgacgc gggctaacgg
     8401 gttgccttca tcgacgtgca gatcgtttcg gccatcttca gcgactcgtc gaaggagtcg
     8461 ataggcttgt cggctcgctg gatgacgctg cagcagagcg aaccgttgtt gacgggtcgg
     8521 cagacttcga gcttgaagaa ggcgtggtcg gtgcgttggt cgacgttgat gtagccgccg
     8581 tcgggaagtt tttcggcgcg cgcgatcgtg cggtctttag cgtcgtcgcc gaggggctgc
     8641 gagtctcccg tcgcgtcggg ggacatcggc atctcgctga agaggaccgt gaatgagggc
     8701 gtgtcgaacc aggctttctt cggttcccac gtcgagtacg cgcctttgac ctcgggcggc
     8761 tttagtgcgg cgctggggag gtcgatcgtg aatgcgacgg tgcctgctgt gctcgccacg
     8821 ttttgaagcg gcgtcgacgc gagctcgtgg ttcgagatgc gctcgggccc ttcggctttc
     8881 gctttcggct tgtcggcgcc ggaaggcgaa ggcgccttgt cgctgccttt gcaggcggcg
     8941 gcggcggccg cgagcagaag aatggttcgc atgagagcga cgtaacatgg aacctgggcg
     9001 gcttggcaca tgtgacatcg gcgcgcagcg agtgagctgc tgctgtcgct tccctctgta
     9061 gagcgactgg gtagtcttgc tgcgcagtgt cgtctctacg cgatcccacg caggccagtt
     9121 tggatgccga tgagaagctc ggaacgccgc ggttgcgtca gttcgttggt gcgatgattc
     9181 cgatcgcggt cgcgtacctc gcggtgttcg tggtggcggc gatcgtgtgg cgcagcgtgg
     9241 cgctcggtgt tgggtctgcg gccatcgtcg tgtatctggc agcgctcggc ggggcgcgcg
     9301 tgtcgcttgc gcgtggcgag gtcgaacggg cggcgctgct gaccggcggc ggcttgctcg
     9361 cgatgatcgg gatcgcggtc tttgcggtgc attttcagta tccggcgttt gcgttgatcg
     9421 cgatcgcggc ggtggtggtg gtgttgccgc atgtggagcg gcgtgtgttg tggtggttcg
     9481 cggggacggc tatcgcggcg gtggtaatcg tgacgaccgt cggtgcgcta ccgcctgcgt
     9541 tcgagcagcc gccgctatgg tttcgcgaga tcgtgctctt gtcgtcgctg ttcgccgcgt
     9601 cgtgcttgat cattttgatg ctcgctgctg atcaccggcg tatgcgcggc ttggtgcgtg
     9661 agtcgacgga gctcgcgggt gtggcgcagc ggcagcgcga tgcgacggat gcgtttttga
     9721 tcgctgcatc gcatcagttc cggacgccgg tgagcacgct gctgctgcag agcgagagct
     9781 tgctgcgtgg agcgccagat gatgcgagca agcggcgcct ggagcggttg atgcgttctg
     9841 cggatcggct gcatctgctc gtcgacacga tgctcgatgt gtcgcgcatt acctcgggct
     9901 cgcttcagct cgaacggacg gtcgtcgatg tcgctgcact cgtgcgcgcg gtcgcagcga
     9961 cgcagcaggc tgggcacgcg agtgatgccc gcgtcgaggt gttgatcgtc gacgacggcg
    10021 gggaaagcgc ggaggagctg cacgtcgagg cagatgcgcg caggctcgag ctgatgacgt
    10081 cgcacttgat cgagaatgcg gttcggcttg gcaagggcgc accggcgcgc atcgaggtgc
    10141 gccgcgacgc acgcgacgtc ttgatcgcga tcaccgatgg cggcaagctg ctcgatgtct
    10201 cgacgcgtga gcggatcttc gatcgcgagg ccctgcagtc gccgcaggcg gagcacgatg
    10261 gcgggccggc ggttggactg tggctcgtgc gcgagctctc gcgcgcgatg ggcggtgagg
    10321 tgtcggcgaa cgaaggcgcg aatggcggca ctcgcttcga ggtgcggctt cctcggagag
    10381 ttgtgtgagc gtggtgctcg tcgtcgatga cgacagcgat ttacgcgaga cgatctgcga
    10441 cgtcctcgag gcgtacggcc atagaacgca tgcggcgcgc gatggcgagg aagcgctggc
    10501 ggtgctgcgg cgtgaagcgg gaatcgagat cgtgttgctc gatctgatga tgcccatcat
    10561 gaacggctgg gagtttcgcg catcgcagct tgctgatcgc gagctcgcgc gcatccctgt
    10621 ggtcgtgatg actgctgccg cggacctgac gcggtcgccg atcgcagcgg cgcaggtcct
    10681 gccgaagccc gtgacgacga aggcgcttct cgacgcgatc gcgcagcacg cgcttgcgcc
    10741 acagtagcag agtgatgcct gatggcacgc gacgtgaaac gccgcgatag atatgggcac
    10801 gttcagcgaa ccagatcctc tcgccgactt cttcacgaga gagcggacgc gcgtgctcca
    10861 gtgtctcacc cgctatgccg atggtgaagg cgctgcggcc ttgggcgaag cgcgagatat
    10921 cctgcgtgcg ctcgacgacg ccgagactca tgtgctgtac ccggcgtttt cgctcgtcac
    10981 gcttcggccg gagacgcagc ggctgctcga tgactcgcgc ggtgctcgcg cggaacagct
    11041 cgccgctctc gatgcgctcg cgcataagcg cgcggtgagg ctccgcaagc tgggtgcggt
    11101 cgcgctcagc gatgagatcc agcaccacgc acggcaacaa atttcgcaga tgctccctgt
    11161 gctctcgagc cagctgccgc gagtacggta ccgatcgatt gtcagcgcct tcacatctcg
    11221 ctacgaaggt gcgcgcgaat tggcaccagt tccggtggag acgcgacgtc agcgcacgcc
    11281 catccaggcg tagttcgcta taatcgcgtc gtgggtgaga ggtcgtcgag ccgtggcgac
    11341 acgcgcgcgg agctcgaacc gctcagcgcc gatagtcgcg cgttcctgca ggaacgcctg
    11401 acgctctttg gccggtggtt cgcgctcgtc gcgatcgccc actacttcat cgccaacacg
    11461 ccgaccgacc tattcctcgg gtatccgttc gattggatca gcgagtactt tgggccacag
    11521 gaacagctcg agctcgccgt cacggcatgc gctgcgctcg tgtgggtagt ggctcgcgga
    11581 acgtcgcgca gcgtgcgtat cctgcgtgtc atcgacgctg cagcgatcgt cgttcccatg
    11641 gcgctattag cgctgctcgt acaccacgcg acggcggcgc acggggacct tggtgaaatt
    11701 gccggcgtgc tgctcgcgtg cacgaacgtc gtcgttgtcc gcgcggttgt cgtgccgagc
    11761 acggcgcgtc gcacactcgt ggtgagcgcg gtggcgtggc tgcccgtcgt tgcggtgacc
    11821 gtctacgcgc tggcaacgag cgatggggcc acgcgcagcg atatcaacat ggcgctcgcg
    11881 accgtgatgt gggccgtcgt gtcgaccgcg tgtgcggcga tcgcttcgcg catcgtgttt
    11941 cgcttgcgcg tcgaggtggc cgaagccgaa gcgttgcggc tcggtcagta cacgctgcag
    12001 cagaagatcg gtcagggagc gatgggcgtc gtgtatcggg cgcaccacgc gctgctccgg
    12061 cggcctaccg cgatcaagat gctcgcaccc gatcgcaata gcgcgacgga tctgcagcgc
    12121 ttcgagaatg aagtgcagca caccgcgcgg ctcacgcacc cgaacacgat cgcgatctac
    12181 gactttggac gctcgcgtga aggcacgttc tattgcgcga tggagctcgt cgatggaccg
    12241 tcgctcgagg aagtcgtacg gcaagacggc gccctgcccg cgtggcgcgt tgtccacctg
    12301 cttcgccaaa tctgtggagc gctcgcggaa gcccacgcga tgggcatcat ccaccgcgac
    12361 atcaagcccg cgaacttgat gctgtgcgag cgcggcggtc agctcgacac gatcaaggtt
    12421 ctggacttcg ggctcgtccg caacctccgg gacctcgaga cggagcacag ggtcgtcggc
    12481 acgccgctct acatggcgcc cgaggcattc gagaaccccg acggcgtcga cgaacgatgc
    12541 gacctctaca gcctcggcgc gaccgcgtac ttcctgctga ccggcactcc gccattctcc
    12601 ggcgcaacga ccgtcgcgat ctgcgccaat cacctcgctc aggtacccga gccgccgtca
    12661 cgtcgcctcg gtgggccgat tgctgaggat ctcgagcgcc tggtgctgcg ctgtttggcc
    12721 aaggccccga gcgaccggcc acgcgcgcgc gagctgggcg agctcttgag cgaatgcgtc
    12781 gatgcgagcg gctggagcaa cgagcgtgtc gccgagtggt ggcgcgcaca cgagcggccg
    12841 gcgacgggcg actccgatcg catgaatgca accgggacgc tcaccgtcga gcccatcgca
    12901 acgcgcgacg ctagctaacg ctcgtcgtcg gtgccgtcga tctggcggag ccttcgcgcg
    12961 agggtgccgc gatcgatgcc caagacatcg gcggcgcgac gctggtgctg ctccactttg
    13021 tcgagcacgt gaaggatgta ccaatcctcg acctcgcgca gcgtcgcggc accatcgacc
    13081 acgcgcgcga gaccctgtgg ttgcgactgc gctttcgtgg catcggcggt gggctcgacc
    13141 gtcgtcgcag gcgtttcgcg aaagccgagc agccagcgac gcagcgtggt ctggagctcg
    13201 cggacatttc cgttccaggg ccgcgctcgc gcctcgtcgc tgtcgagcca cgcgaggaac
    13261 cgcgcctggt ggacgatcaa gcggccgacg ttcgccgtgc tcgcgccgct tcgcgccatc
    13321 ggtgctgact gctgctcgaa cattcgctcg gcgatcggcc gcagatcctc gagccgctcg
    13381 cgcagcggtg gcaacacgat cgtgattccc gccagccgct cgaacagatc gcggcgaaat
    13441 gcgcctcgct cgaccatcgc gtgcagcggc tgcgatgtcg cggagacgta acggacgtcg
    13501 accttcttgc cttggtttgc gccgagcggc gtgacgacgt catcgtcgat gacacgcaag
    13561 agctttgcct gcagcaccgg cggcaaatcg gcgagctcgt cgagaaacag cgtgccaccg
    13621 tcggctgcgc tgatcttgcc ttcgcgatcg cccacgctgc cggagaacgc acccttcttg
    13681 tggccgaaca gctcggactc gaggagcggc tcgggaatcg cggcggcgtt gagcacctgc
    13741 acgcgatccg gatcaggatc cgacgcggcg atcgcttgcg ccacgagctc cttgcccgtt
    13801 ccggtctcgc cgaggacgag cacgttctgc tcgcgaatga gcgggcgcat gctgatcgcg
    13861 tcgtagacgg attccccgtg gcacgcgcgc cagaccgcgg cccgcacgcg agcaatcgcc
    13921 tgcgacgtgc cagcgatgtg atgcaggctg gcggcgcagc gagccgccga cgccgttacc
    13981 tcgcgcacgg cctcgcggga cagccgaagc tcgctgaaga tgcgctcggg acactgccgc
    14041 gggtcgagga agctcgcgac tgcttcgtgc gcgagctcgt cgacgacaaa cgcgaccagc
    14101 agacgacgcg ccaccttgtg ttgtcccttg cgtgcgaggt cctccaaggt cgccgtgcca
    14161 cgcgcgcgag ctgcttgcac cgccgcatgg gcttcctcgc gccccgctac cgtctcgaag
    14221 ggattgccaa ggacaacctc cgcgacacgt gccagctcac caacgtccat ggaggctcat
    14281 cgtctcgatc gcgtctcgcg ctgtcaatcg aagctgcgat ctgccgcagc cggttgcgat
    14341 ttgcggcgtc gctggcgctg ccacgccgct gcctcgtcga ggaatccgtt ggtcgcgccc
    14401 ttgcacgagg ccgcggcatg tccttcgcga cgcgcgcaat cctcgtcggc ggtctcctcg
    14461 ccgcgatcgc cgtgctcgcg acgtgccaac acctgccact gcttacctgg acaccgcagc
    14521 tcgtcgagcc gcggacctat ccgctgctgt tcgggcagct gttcgtcgcg tggctggcgc
    14581 tcgcggtgtc gccgcgcatc tggcgccgtc ccattctcgt catcgcgtcg ctcgcgtcgc
    14641 tcgtcacgct gagcccgctg ttcgccgttg tcgctgtcgt ccatgccggg ctgacaaccg
    14701 cggtgctgcg cgcgccactt ccgcgcatcg tcgtgcttgc attcctcgtc ggctcggtgg
    14761 ctgccgccgt cgcggtcggc aatgcagagc tcggcgacga ggcgcgcagg ttggcgatcg
    14821 tcttcgcgct cgggcagacg ttccgcctct tcgcgctcta tcacgccgct cgggtcgccg
    14881 gcgcgcggcc gttgtcgctc gtcgacacct tcctctatct ccacctgctg ccgtgcaacg
    14941 tgatcgtgcc gtacatgctg tgcatcccgc gcgtcgagcg attccgcaag tcgctcgata
    15001 cgccgcgcgc attcgacgtg cggctgctcg ccgcgggcgc ggtggtcggc gtgctcgttc
    15061 acgcggggtt gcgcgtcttc gatccgaaga tcgcgcttct ccacgcggcg caggcccacg
    15121 acattccgcg cgttgccgcg ttcgcactcc tcttttatcc gctaagcggc gtgctcggca
    15181 tcctcgggcc cgcgtggatt ctgacgggga tgatgcgcct cgtcggcatc gacgtcgatc
    15241 cgccgttcga tcacccgctc tcctcgacga gctttctcga gtggtggcgg cgttggaaca
    15301 cccactttcg cgacctgctc gtcgatctgt tcttctttcc cgtcgtgctc ggccgcctgc
    15361 gcaggcgccg ccgcatcgcc acggtcattg gcgcggccgc cgtgtttctg gtcggcagca
    15421 cgctgtttca catgcccaag cacatgctgc tcgtcgcgca cggctgggag atgccgtggg
    15481 ggctgttcgc cgagaacgtt gtctcgttcg tgttcgttac cgttggtctc ctgcaaggcg
    15541 cgcgaaggcg tccgccacgc cggcgtctca ccgcgatctt cgtgacgtgg atcgtcgtct
    15601 tcgtggccgt gcacattggc tatctcgtcg gctggtcact ccaccctgac cgcctcccaa
    15661 aggagagctc gaaactatga gcatcgattc tcgtcctgcg ctgcgcgcat tcatcagtga
    15721 cctcgccgtc ggcgcgatgt tcaccgacga cgtagacctc gtcaaagagg gcatcgtgtc
    15781 atcgcttcag ctcatggagc tgatcgcgtt cctcgaggat cgcttcggcg tggtcgtcac
    15841 ggcagtcgac ctgcacaacg cgcggctcgc cagcgtcgcg acgatggcag cgctcatcga
    15901 ggagttctcg tgaacatgga catcttcgca atgatcgacg cgcacgcggc acgcgctccg
    15961 tccgccctgg tcttcatcag ctcggcgggc cggctgacgt ggggtgagct cgacgcccgc
    16021 gccaacgcgc ttgcaggtca tctccaccgc accctcgtgc gcggtagcgc cgtcgtcgtg
    16081 cgcggtcaga aggagcccgg catgttgatc gcgctgctcg cgtgcgcgcg cgccggcatg
    16141 gccttcgtgc cgatcgacga agcgttgccg atggcgcggc aagcgctgat cgtacggcgc
    16201 tcgcgggcac cgctcatcct cgaggctgcg gcgttgccgg ctgtcgaaaa tagcgagccc
    16261 gcacccgcgg cggcgcgtct cacgcgcgat gacatcgtct acatcatgtt cacgtcgggc
    16321 tcgagcggtg agccgaaggg cgtaccgatc acgttcggca atctgcaggg cttcctcgac
    16381 tgggcgtgcg aggtccatcc gatccgcgga ggcaaacgct tcctcgatca ggcaccgtac
    16441 tcgttcgatc tctccgtgct cgatacgtgg ggcgcgctcg taacgggcgg gagcctgttc
    16501 gcggtcgacc gcgccacggt cgcgaatccg ctgctgctcg cgccggcgct tgcagcggga
    16561 ggcatcgaag tctgggtgtc gacgccgtcg ttcgcggagc tgtgcctcgg cgatcgccgc
    16621 ttcgacgccg cgatcttgcc gcgtctctcc tgctttctgt tctgtggcga accgctcacg
    16681 cccgcgaccg cactgcgtgt caagcagcgc ttccctgcgg cgcgcatcta caacctctat
    16741 gggccgaccg aagcgacagt cgccgtgacc tccgcgctga tcgccgatca ccacctgtcc
    16801 cgagatgcac cactgccact cggcacgccg ctccccggca cgcgcgtctt cgtcgcgaac
    16861 gacgctggcg acccggtgcc cgacggagag tccggggagc tcgtgatcga aggtccgtgc
    16921 gtcagccccg ggtatctcga cgcacccgag ctcacagcac gctcgttctc cgggcacgcg
    16981 ccgcgccgtc actaccgcac cggtgatgcg gggcgagttg tcgacggcga gcttcggttc
    17041 gaaggccgcc tcgatgggca gatcaagctc ttcggccatc gcatcgagct cgacgacatc
    17101 gccgccaatc tcaccgcgct tccgaatgtc gcccgtggag tcgtcatcgc cgtcgaggaa
    17161 ggcgggcgcg ccgtcgcgct cgtcggcttt gtcgtcggtg cgccgggcgc gcgcctcgac
    17221 ccgcgacagc tgcgcgacga gctcgccacg cgcgttccct cgtacatggt gccgcgcgag
    17281 atccgcgtcg tcgacgcact gccgatgacc gtcaacggca agctcgatcg ccgctctctc
    17341 gccgagctgt ctcgtcagcg gccgtagcta catcttgcgg ctgtatgcgc gaaccgcgag
    17401 cggcgcgaag atcgcgaccg tcgcggcgca gccgaacagt gcccaaccga cgctgctcgt
    17461 cacctcgccg tgattcatga gatcgcgcac cgcgcgaatc accagcgaca cggggttcac
    17521 gttgacgaac gt
//
LOCUS       DATLBZ010000141         2862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9757204, whole genome shotgun sequence.
ACCESSION   DATLBZ010000141 DATLBZ010000000
VERSION     DATLBZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9757204"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..486)
                     /locus_tag="VMZ53_07610"
     CDS             complement(112..486)
                     /locus_tag="VMZ53_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28357.1"
                     /translation="MSATDPLTLRLPSWLCNSFDLPRAIANEHRARGRTTGACRFDQF
                     EMRSHERAFVKTVLARRSNLWLFRTNQRRWCGDFIAIDMSSRRVDRRAYVMELKTGDR
                     LVIGGARLQCAQYGAAVEELHG"
     gene            complement(749..2068)
                     /locus_tag="VMZ53_07615"
     CDS             complement(749..2068)
                     /locus_tag="VMZ53_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02996 domain-containing protein"
                     /protein_id="HUS28358.1"
                     /translation="MKLWRARGKKTEYVDLKLEGKTLTTVAAEHWGTPTTKKKSFKTP
                     ALAKAGLETAIAEQRELGFRELGEIDPPAFEVPRDAGLEAEIRKHRDDPAPYLVYADW
                     LQGKGSKFGEMIVFANRKKQKEAAAIAAKVCALPPKMGTATWRNGLWNTLHLQNDLEW
                     MEGFDILRFVKAVFSSPLCAALEELKIGILQWDNSEQNVVLAEAGKHAWAKDLLRLRL
                     GDVGDADVDMDHHSVGDVGKVITKSFPSLESLFLHSGGDYGSNSEFGFGGLDLPKLKE
                     LTIETCAMSRKRMKSLSTAKLPALERLVLWFGERERGANATLADVMPVWDGKLFPRLR
                     HLGLCNTELVLDFIRLLPEGKLAKQLVSLDLSKGTLGDDGITELAEVAAKFPALETLC
                     VDDSWITPAGVKALKKAFPRVKISAKDQQALLDPGEYGTDRYVHVSE"
     gene            2186..2755
                     /locus_tag="VMZ53_07620"
     CDS             2186..2755
                     /locus_tag="VMZ53_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HUS28359.1"
                     /translation="MRNVIQLAHLSLDGYLAGPSGEMDFLTFDHEIADHVYPLMRTVD
                     TGIYGRTTYQMMEGYWPSVLDDANATDGEKSHARWYADVKKIVASRTLPAQTGHTTIA
                     GDDIVSVIAAAKQQPGGEMMIFASPTLSHALSAAGLIDEWRLTWQPVIVGGGLPLFGG
                     KETRSKLELRSSKTFKSGVVATHHVRSRP"
BASE COUNT          464 a          963 c          919 g          516 t
ORIGIN      
        1 ttggagctca agacgggtgg acggctcgtg gtcggtggtg cgcacttgca gtacgcgcaa
       61 cacgctgcgg cggtcgccga gctcgttgcg cgcggcattc tcgacatcgg ctcatccgtg
      121 gagctcctcc acggccgcac cgtactgagc gcactgcagg cgagcgccac cgatcacgag
      181 ccggtcgccc gtcttgagct ccatcacgta cgcgcggcga tccacgcgac gcgacgacat
      241 gtcgatcgcg atgaagtcgc cgcaccaccg gcgctggttc gttcgaaaca gccacaggtt
      301 gctgcgtcgt gcgagcacgg tcttcacgaa cgcacgctcg tgactgcgca tctcgaactg
      361 gtcgaatcgg caagcgccgg tcgtcctgcc gcgcgcgcga tgctcgttcg cgatcgcgcg
      421 cgggaggtcg aacgagttgc agagccagct cggaagtcga agcgtcaatg ggtcggtcgc
      481 ggacatgctg cgtcactttc gtgcgagctg atcgatcagc gcgcggcagg ctcgacgcgg
      541 cacggccgct cggtcgagca attgcgcggc gagccgcaga tcgccgttgt ctgcggcttc
      601 aagcgcctcg aacgcgcggc tctcggcgcg gcgctgggcg cggcggtgct gttgggtcaa
      661 cgacatgacg gctctccatc cttcgcgtcg agcgaaggtc gagcaagcta gcgttcgcgg
      721 gtcgatccgg caaacgcttt tcgcgcggtc actcggagac gtgaacgtag cgatcggtgc
      781 cgtactcccc gggatcgagg agcgcctgtt gatccttcgc gctgatcttg acgcgcggga
      841 acgccttctt gagcgccttg acgccggcgg gcgtgatcca gctgtcgtcg acgcacaacg
      901 tctcgagcgc ggggaacttc gcggcgacct cggcgagctc cgtgatgccg tcgtcgccga
      961 gcgtgccttt cgagaggtcg agcgagacca actgcttcgc gagcttgcct tccggcagca
     1021 ggcggatgaa gtcgaggacg agctcggtgt tgcacagccc gaggtgccgg agccgcggga
     1081 acagcttgcc gtcccacacc ggcatgacat ccgcgagcgt cgcgttcgca ccgcgctcgc
     1141 gctcgccgaa ccacaggacg agccgctcga gtgcggggag cttcgccgtg gacagcgact
     1201 tcattcgctt gcgagacatc gcgcaggtct cgatcgtcag ctccttcagc ttcggcagat
     1261 cgagcccgcc gaacccgaac tccgagttcg agccgtagtc accgccggag tgcaggaaca
     1321 gcgactcgag gctcgggaag ctcttcgtga tcacctttcc gacgtcgccc accgagtgat
     1381 ggtccatgtc gacatcggca tcgccgacat caccgaggcg aaggcgcagc agatccttcg
     1441 cccacgcgtg cttgccggcc tcggcgagga cgacgttctg ctcgctgttg tcccactgga
     1501 ggatgccgat cttgagctcc tcgagcgcgg cacacagcgg cgagctgaac acggccttga
     1561 cgaagcgcag gatgtcgaag ccttccatcc actcgaggtc gttctggagg tggagcgtgt
     1621 tccacaaccc gttccgccac gtcgcggtgc ccatcttcgg gggcagcgcg cacaccttcg
     1681 cggcgatcgc ggcagcttcc ttctgcttct tgcggttcgc gaacacgatc atctcgccga
     1741 acttgctgcc tttcccttgc agccagtccg cgtagaccag atacggcgcc ggatcgtcgc
     1801 ggtgtttgcg gatctcggcc tcgagcccgg catcgcgcgg cacctcgaac gccggcggat
     1861 cgatctcacc gagctcgcga aacccgagct cgcgctgctc cgcgatcgcc gtctcgaggc
     1921 cggccttcgc gagggccggc gtcttgaacg acttcttctt cgtcgttggg gtcccccagt
     1981 gctcggcggc gacggtggtc aacgtcttgc cttcgagctt gaggtctacg tattcggttt
     2041 ttttgccgcg ggcacgccag agcttcaccc cccgacggta agctacgagg ccagatttcg
     2101 cacggttacg acgtcgatgt tttttcgcgt ccgttagtcg atcgccgccc gcgtggttcg
     2161 tcgctcgggg aaaggacacg aacccatgcg caacgtcatc cagctcgctc acctctctct
     2221 cgatggctat ctcgccggcc ccagcggtga gatggatttt ctcaccttcg accacgagat
     2281 cgccgaccac gtttaccccc tgatgcgcac cgtcgacacg ggcatctatg gccgcaccac
     2341 gtaccagatg atggagggct actggccatc tgtactcgac gacgcgaacg cgaccgatgg
     2401 cgagaagagc cacgcgcgct ggtatgcgga cgtcaagaag atcgtcgcgt ctcgtactct
     2461 acccgcgcag acgggccaca ccacgatcgc cggcgacgac atcgtctcgg tgatcgccgc
     2521 cgcgaagcaa caaccgggtg gcgagatgat gatctttgcg agcccgacgc tgtcgcacgc
     2581 gttgtcggcc gcgggcctga tcgacgagtg gcgcctgacc tggcagcccg tgatcgtcgg
     2641 cggcgggctg ccgctgttcg gcggcaagga aacccgtagc aagctcgagc tgcggtcgtc
     2701 gaagacgttc aagtcgggcg tcgtcgccac tcaccacgtc cggtcgcgcc cgtgaaggtc
     2761 atgatcaccc tccagggcga cgcgccgatc gatccgatcg acgcgtacct caccgcgcaa
     2821 ggcgcatccc tcgtcggcgc cgaacggctc gatccggacg ag
//
LOCUS       DATLBZ010000142         9969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6030564, whole genome shotgun sequence.
ACCESSION   DATLBZ010000142 DATLBZ010000000
VERSION     DATLBZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6030564"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VMZ53_07625"
     CDS             <1..594
                     /locus_tag="VMZ53_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS28360.1"
                     /translation="GAQRTKAIVSALHNYSRTDDESVVEFDIDRSIDDSLELLRHLLK
                     QNVTVVRKYGEVGRVRGHAGQINQVFMNLLTNAAQALAGRDSATITIETRGEPEGVEV
                     KILDNGPGIPPEVLPRIWDPFFTTKDVGEGTGLGLSIVHELVERHGGTIECKTKVGQG
                     TTFTVKLPRKIQMSDRAKRPTGGGSEPKRETAVTKPG"
     gene            complement(596..1954)
                     /locus_tag="VMZ53_07630"
     CDS             complement(596..1954)
                     /locus_tag="VMZ53_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HUS28361.1"
                     /translation="MGGGRCIFIVLVAGCRLHFAELDDAALGGDGTAGDSTTDGGQGA
                     TCARAIPTMPTFWVDTNAGLDTNPGTQAAPTKTIARALSLTSASGGTIIVAPGNYLES
                     LDISTTQPLLLLSEQRYAARMQRVNCAGCSELTVEGFEITGSALPLVSITAGARVTIR
                     DNVVFDGSSAGIRITSGSTEIVIDSNVVYDSFASHIHVNDADNVTIRSNVLFDDNGPS
                     GGLAKLWLETAANTKVEGNIVFRSLGDNASYGLISLRDTTGATVVENNLVAGSPNATN
                     VYASIGFDMATGTSLIRHNTFIGPMPGTAFGLGAGAMMTGSNFTVVNNLWASTGTLQP
                     FTDGNAVALVALRHNLYWNAPLGPFQPGGSPNPSGDTQAIVANPGLPATLPAAPTRIA
                     TGFVGDAKTTCEVHAQLVEAMAKIATTSPAKGAADPTQSPAVDIRGNARPALPAIGAY
                     EP"
     gene            2076..2279
                     /locus_tag="VMZ53_07635"
     CDS             2076..2279
                     /locus_tag="VMZ53_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28362.1"
                     /translation="MKTIDPSELHGVVGGMRWEQYGASKNVEDRRPAWAKRRDDAWFR
                     SLDQRESGVTTNVPLPPRRPRGL"
     gene            2433..3938
                     /locus_tag="VMZ53_07640"
     CDS             2433..3938
                     /locus_tag="VMZ53_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS28363.1"
                     /translation="MWHQPAQSPFRRFAAAQVEGGSGTPDNASEPSALREVPADAGIV
                     LIVDDEGPIVESLTKIFKREGLNVLAATDGNSALDILRKHRVGVLLTDLMMPNTSGMD
                     LLRAAKTIAPETEVVLMTAYGTVETAVDAMKEGAYDFVTKPLKRAHVVRIVRNALEKQ
                     SLLVENRSLKAQLAEKRRRAIIGTSLAWRRTMDIVMQAAPSEATVLLLGESGTGKELL
                     ARALHDNSARAKGPFVAVNCAAIPESILEAELFGYEKGAFTGAATARDGRFEAAHGGT
                     LFLDEIGEISRHVQVKLLRVLQEGEIERLGASGKTRRIDVRVVAATNVNLGEEVKAGR
                     FREDLYYRLNVIPVNVPPLRDRRDDISLLAQHFVHVYAEKNGKAIGGCSPTALERLTE
                     YGWPGNVRELENAMERAVVLTRAGHNMIDEDALPREIRDATPGTASATSLTFPIGMPL
                     AEIEMRVIHETLRHTRGDKRLTAKLLGIATRTIYRRLESTDGGGEPEGEDQ"
     gene            3942..4871
                     /gene="gspC"
                     /locus_tag="VMZ53_07645"
     CDS             3942..4871
                     /gene="gspC"
                     /locus_tag="VMZ53_07645"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HUS28364.1"
                     /translation="MQDLVKRYFWVLGAIVVLVCAVFAAKCTSHVIEAKALGDSSKTP
                     RVVPTVPSPMQKPATPVRSKDGSQFATRNMFCSECTPQPTVATNVDPSQIQTTSLPLV
                     LLATMVATDAKDSYATIVNTENQKQGAFSIGDPIPGASGKIKEIHYKYVDFENSGHTE
                     RLVLAGATAPVTSTPVAAETPPPTDENKDDLQASIDAGIKKIDDSNYEIDKSLVDKIL
                     ANPMGVAKGARVVPAVKNGQPDGFKLYAIRPSSVYSKLGLANGDTLQSINGFELNSAD
                     AALNAYTKLREATSLELNVTRRGKPMTLKYQIR"
     gene            4875..7220
                     /gene="gspD"
                     /locus_tag="VMZ53_07650"
     CDS             4875..7220
                     /gene="gspD"
                     /locus_tag="VMZ53_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HUS28365.1"
                     /translation="MKTTRSFVILILVSLAAPVFAQPAPTTPPAGGDASADDALYSCK
                     KRTGLVAVTFKPETELKDLITWVMGFTCKNFMLDPRIVSTGKKVTVIAPNKMTAQEAY
                     NLFLAALSTMQLTVVPKGNVLRIVESATAKSETIPIQKKGVPGNQDQMVRYILRPTYT
                     QTETLRAALDSVRSPAGNVSTAGNVIIITDYASQVRDMLSLAKSLDIAGNNEGIYTIP
                     VKNSDAQTLAQKINEILGVTGAGGGGGGGGARGGGKAGGPADGGADNGAGTPSKILVD
                     DRTNTLIVVSSEAGYMRVKALVDRLDIVLDTEGGSAIRVYPLENALAEELAATLTNVL
                     SGQGQQGQQRPGQPPAAANTPRPAGAVGGEFGSSLEGTVRVIGDKPTNSLIVMSSGRD
                     FIAVRDVIRRLDQPRRQIFIEALILEVQLNKELDIGTSSHGGLPVAGGDAIVLGGVQT
                     PTLKSINPSSLAALTGLVGGLIGTPLANSQTFLGTSIPSYAVLFQALANQDNSNILSA
                     PHIIAIDNEKAEFSVGNNIPYKAGLSFGGLPGTTGATNPLGSVGTNIQREKLNLTLNV
                     TPHISSNDSVRLEIEQETKDLGGKDPELGPTWAERKLKTQVVVHDQESVVIGGLIQER
                     EIYNATKVPLLGDIPLLGYLFKYTTKQKKKTNLLILLTPYIIKDQLDLSTIKERKVRE
                     YREFAASFANLNDKKYEPRIDYRRKRGLLEEINRAVQSVEEDQAVLNATGQRINVMEG
                     GVEFGPSSIDSPDDDKKPDAPKTDAPKTDAPKTAPAPEPKK"
     gene            7223..9028
                     /gene="gspE"
                     /locus_tag="VMZ53_07655"
     CDS             7223..9028
                     /gene="gspE"
                     /locus_tag="VMZ53_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HUS28366.1"
                     /translation="MATPSSYMFPSDQSAGPARIGEILVRQCGVSPDSIERALGKQRE
                     EGGLIGEVLVRLRLIDEDQLAMALAIQQDMTYLRELPRAEDIPAELIDKLPINFARQR
                     LILPLGRDGANRVMVAIADPYKVEVIDAVSVLLGEPVEPVVASATKITDHINKVYSSL
                     RGGAELEAGAKKDDEGEEFGTNADELVDMLDVNDEAPIINWVNGLVFEAAKKGASDIH
                     IRPGEKDIIVRYRVDGVLREVKRAPKKFQPSIIARVKIMAGLNIAEKRLPQSGRIRRK
                     MAGRDVDMRVETAPTASGESVTIRLLDRSSVLLGLADIGFAEDQLTQIRSIIARPHGI
                     LLVTGPTGSGKTTTLYACISEINAPDINILTVEDPVEYQLEGISQTQVNSKIDLTFAS
                     GLRSFLRQDPDVIMVGEIRDRETAEVAITASLTGHLVFSTVHTNDAAGGITRLVDMGI
                     EPFLVASSLVGLLAQRLVRRPCYECARPVRPSEEIVNELGLDPALFFRPGGGYRFPAV
                     KGQRPPPPGTVFEPVGCPACQQLGYKGRTGIYELLMINEPVRRLALEKADAGAIRKSA
                     EDHGMVSLRGDGARKVVLGMTTPEEVMLMTAESND"
     gene            9028..>9969
                     /locus_tag="VMZ53_07660"
     CDS             9028..>9969
                     /locus_tag="VMZ53_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUS28367.1"
                     /translation="MPMYAYKGVANNGKTVTGVRDADSPKTLRQVMRKDGVLVTSFEL
                     SKGGKAQKEQAAKKGGLNRDVDLGGLIGGVGKTEIAAFTRQMATLIRSGIPLADSLAA
                     MVEQQTNVRFKTPLSEVRAAVNEGSSFADALAKHPKLFDELFVSMVRAGELAGNLDDV
                     LGRLADFLEASQKLKSKIQGAMIYPAVMVVVGTGIMAVLMIKVIPQITSMFTSQGKTL
                     PLNTRFLIWTSRFMGNYWLLILIGMIIAGILFSKWKGSKEGKPVWHRFVLKLPVLGQL
                     VRTINVGRFARTLGTMLQSGVPMLRALDTAKQIVGNVL"
BASE COUNT         1888 a         3313 c         3163 g         1605 t
ORIGIN      
        1 ggcgcccagc gcaccaaagc aatcgtgtct gcactccaca actattcgcg caccgacgac
       61 gaatccgtcg tcgagttcga catcgatcgc tcgatcgacg actcgctcga gctgctgcgc
      121 cacctgctca agcagaacgt caccgtcgtg cggaagtacg gcgaggtcgg gcgcgtccgc
      181 ggtcacgcgg gccagatcaa ccaagtcttc atgaacctcc tgacgaacgc cgcgcaagcg
      241 ctcgccggac gcgactccgc aacgatcacg atcgagaccc gcggcgagcc ggaaggtgtc
      301 gaagtcaaaa tcctcgacaa cggccccggc atcccacccg aggtgctgcc tcgcatctgg
      361 gatccgttct tcacgacgaa agacgtcggc gaaggaaccg gcctcggcct ctcgatcgtg
      421 cacgagctcg tcgagcgcca cggcggtacg atcgagtgca agacgaaggt cggccaaggc
      481 acgacgttca ccgtgaagct gccgcgcaag atccagatga gcgatcgcgc gaagcggccg
      541 acgggtggcg gctccgagcc gaagcgcgag acggccgtga ccaaaccggg ctgagctacg
      601 gctcgtacgc gccgattgca ggtagggcag ggcgcgcgtt gccgcgaatg tcgacggccg
      661 gcgattgcgt tggatccgcc gcacccttcg ccgggctcgt ggttgcgatc ttcgccatcg
      721 cttcgacgag ctgcgcgtgg acctcgcacg tcgtcttcgc gtcgccgaca aagcccgttg
      781 cgatccgcgt gggtgcagcc ggcagcgtcg cgggcagacc gggattcgcg acgatcgctt
      841 gcgtgtcacc cgacggattc ggcgagccgc cgggctgaaa cggaccgagt ggtgcattcc
      901 agtacagatt gtgtcgcagc gcgacgagag cgacggcgtt gccgtcggtg aacggctgca
      961 gtgttcccgt gctcgcccac agattgttga cgaccgtgaa gttgctgccc gtcatcatcg
     1021 cgccggcgcc gaggccaaac gcggtgccgg gcatcggacc gatgaacgtg ttgtgacgaa
     1081 tgagcgaggt gccggtcgcc atgtcgaacc cgatcgacgc gtacacgttc gtcgcgtttg
     1141 gcgagcccgc gacgaggttg ttctcgacga cggtcgcgcc cgtcgtgtcg cgcagagaga
     1201 tgagcccata cgacgcgtta tctccgagcg agcgaaagac gatgttgccc tcgaccttcg
     1261 tattcgcggc ggtctcgagc cagagcttgg caagaccgcc ggacggccca ttgtcgtcga
     1321 acagcacgtt actgcggatc gtcacgttgt cggcgtcgtt gacgtggatg tgcgaagcga
     1381 acgagtcgta aacgacgttg ctgtcgatca cgatctccgt cgacccgctc gtaatacgaa
     1441 ttcccgccga agagccatcg aacaccacgt tgtcgcgaat ggtcacgcgc gcgccggcgg
     1501 tgatcgacac gagcggaagc gcgctgcccg tgatctcgaa tccctcgacg gtcagctcgc
     1561 tgcaccccgc gcagttcacg cgctgcatgc gcgccgcata acgttgctcg gagagcagca
     1621 gtagcggttg cgtcgtgctg atgtcgagac tctcgaggta attgccgggg gcgacgatga
     1681 tcgtgccgcc gcttgcactc gtgagcgaga gggcgcgcgc gatcgttttt gtcggtgcgg
     1741 cttgcgtgcc gggattcgtg tcgagacccg cgttcgtgtc gacccagaag gtcggcatcg
     1801 tcgggatcgc gcgagcgcac gttgcgcctt gtccaccgtc ggtcgtcgag tccccggccg
     1861 tgccgtcgcc gccgagcgct gcgtcgtcga gctcggcgaa gtgcagccgg caccccgcca
     1921 cgagcacgat gaagatacag cggccacccc ccaccgcacg atgatagctc cacacgcgtt
     1981 tcgatcaggt tgccaggcca atcgaagagc cactgtcacc ttgccaaaga gacgcgtact
     2041 tgtggctggc accatgcgcg catgtcgtcg atgccatgaa gaccatcgat ccatctgagt
     2101 tacacggtgt tgtcggcggt atgcggtggg agcagtacgg cgcgtcgaag aacgtcgagg
     2161 accgtcggcc cgcgtgggcg aagcgccgcg acgatgcgtg gtttcgatcg ctcgaccagc
     2221 gcgagtctgg cgtgacgacg aacgtgccgt tgccgccgcg ccgtccccgc ggcctctgag
     2281 catccgcggc ggctttgtca gattgtcagc gccctccgga cagcccgaca cgttggcaca
     2341 aacgccgctc gctgcaaggg aaagacgcga ggtattccag caagttacct ggaatctaac
     2401 ccggcacggc gcttgttaat gtccacacga gtatgtggca ccagcctgcg cagagtccct
     2461 tccggcggtt tgccgcagcc caggtcgagg gcggtagcgg gacaccggac aacgccagcg
     2521 aaccctctgc actgcgcgag gtgccggccg atgcgggcat cgtgctcatc gttgatgacg
     2581 agggaccgat cgtcgaatcg ctcacgaaga tcttcaagcg cgagggcctg aacgtcctcg
     2641 ctgcgaccga tggcaacagc gcgctcgaca tcctgcgcaa gcatcgcgtc ggcgtgctgc
     2701 tgaccgatct catgatgccg aacaccagcg gcatggatct gctgcgcgcc gcaaagacga
     2761 tcgcgcccga gaccgaggtc gtgttgatga cggcgtacgg cacggtcgag accgcggtcg
     2821 acgcgatgaa agagggcgcg tacgacttcg tcaccaagcc gctcaagcgc gcgcacgtcg
     2881 ttcgcatcgt tcgtaatgca ctcgagaagc agtcgctgct cgtcgagaac cgcagcctca
     2941 aagcacagct cgccgagaag cgccgtcgcg cgatcatcgg cacgtcgctc gcgtggcgcc
     3001 gcacgatgga catcgtcatg caggccgcgc catcggaggc gaccgtgctg ctgctcggcg
     3061 agagcggcac tggcaaggag ctgctcgctc gcgcgctgca cgataattcc gcgcgtgcga
     3121 agggcccgtt cgtcgcggtc aactgcgcgg cgattcccga gtcgatcctc gaggccgagc
     3181 tgtttggtta cgagaagggc gcgttcaccg gcgccgcgac cgcgcgcgat ggtcgcttcg
     3241 aagctgcaca cggcggcacg ctgttcctcg acgagatcgg cgagatcagc cgccacgtgc
     3301 aggtgaagct gttgcgcgta ctgcaagagg gcgagatcga gcgcctcggc gcgagcggca
     3361 agacccgccg catcgacgtg cgcgtcgtcg ctgcgacgaa cgtgaacctc ggcgaggaag
     3421 tcaaagccgg ccggttccgc gaagacctct actaccgcct caacgtcatt cccgtgaacg
     3481 tcccgccgct tcgcgatcgc cgcgacgaca tctcgctgct cgcgcagcac ttcgtccacg
     3541 tttacgccga gaagaacggc aaggcgatcg gcggctgctc gccgacggcg ctcgagcgcc
     3601 tcaccgagta cggctggccg ggcaacgtgc gcgagctcga gaatgcgatg gagcgcgccg
     3661 tcgtgctcac gcgcgctggt cacaacatga tcgacgaaga cgcgctacct cgggagatcc
     3721 gcgatgcgac gcccggtacc gcgtcagcga cgtcgctgac gtttccgatc ggcatgccgc
     3781 tcgccgagat cgagatgcgc gtcattcacg agacgctgcg ccacacccgt ggtgacaagc
     3841 gcctcaccgc gaagctcctc gggattgcaa cgcgtacgat ctaccgccgt ctcgaatcta
     3901 ccgacggcgg cggtgagccc gaaggcgagg accaatagtc catgcaggat ctagtcaaac
     3961 gatacttttg ggtgctcggc gcgatcgtcg tgctcgtctg tgccgtcttc gccgccaagt
     4021 gtacgagcca cgtgatcgaa gccaaggcgc tcggcgactc gagcaagact ccgcgcgtcg
     4081 tgccgacggt tccgtcgccg atgcagaagc cggccacgcc cgtgcgcagc aaagacggct
     4141 cgcagttcgc gacgcgcaac atgttctgct cggagtgcac gccacagccg acggtcgcga
     4201 cgaacgtcga cccctcgcag atccagacca cgtcgctgcc gctcgtgctg ctcgcgacga
     4261 tggtcgccac cgatgcgaag gatagctacg cgacgatcgt caacacggag aatcagaagc
     4321 agggcgcgtt ctcgatcggt gatccgattc cgggcgcgtc cggcaagatc aaagagatcc
     4381 actacaagta cgtcgacttc gagaactcgg gccacaccga gcgcctcgtg ctcgccggtg
     4441 ccacggcgcc cgttacctcg acgcccgtcg ctgccgagac gccgccgccg accgacgaga
     4501 acaaggatga cttgcaggcc tcgatcgacg ccggcatcaa gaagatcgac gacagcaact
     4561 acgagatcga taagtcgctc gtcgacaaga tcctcgcgaa cccgatgggc gtcgcgaagg
     4621 gcgcgcgcgt cgtgcccgcc gtcaagaacg ggcagcccga tgggttcaag ctctacgcga
     4681 ttcgcccgag ctcggtgtac tcgaagctcg gtctcgcgaa cggcgacacg ctgcaatcga
     4741 tcaacggctt cgagctgaac tccgccgatg ccgcccttaa cgcttatacg aagctgcgcg
     4801 aagcgacgtc tctcgagctc aacgtcactc gccgcggcaa gccgatgact ctcaagtatc
     4861 agattcggtg acctatgaag accacacgta gctttgtcat tctcatcttg gtgtcgttgg
     4921 cggcaccggt cttcgcacaa cctgcaccga cgacgccgcc cgcaggtggc gacgcctctg
     4981 ccgacgacgc gctctacagc tgcaagaagc gcacggggct cgtcgccgtc acgttcaagc
     5041 ccgagaccga gctgaaggat ctcatcacgt gggtgatggg cttcacgtgc aagaacttca
     5101 tgctcgaccc gcgcatcgtc tcgacgggca agaaggtcac cgtcatcgct ccgaacaaga
     5161 tgacggcgca agaagcctac aacttgttcc tcgccgcact gtcgacgatg cagctcacgg
     5221 tcgtgccgaa gggcaacgtg cttcgcatcg tcgaatcggc caccgcgaag agtgaaacca
     5281 ttccgatcca gaagaagggc gtgcccggca atcaggacca gatggtccgc tacatcttgc
     5341 gcccgacgta cacgcagacc gagacgttgc gcgccgcgct cgactccgtt cgctcccccg
     5401 cgggtaacgt gtcgacggca ggcaacgtca tcatcatcac cgactacgcg agccaggttc
     5461 gcgacatgct ctcgctcgcg aagtctctcg acatcgccgg caacaacgaa ggcatctaca
     5521 cgatccccgt caagaactcc gacgcgcaga cgctcgcgca gaagatcaac gagatcctcg
     5581 gcgtgacggg cgcaggcggc ggcggtggtg gcggcggtgc acgaggcggc ggcaaagcgg
     5641 gcggccccgc ggacggcggt gctgacaacg gtgccggcac gccgtcgaag atcctcgtcg
     5701 acgatcgcac gaacacgctg atcgtggtgt cgagcgaagc gggctacatg cgcgtgaagg
     5761 cgctcgtcga tcgcctcgat atcgtcctcg ataccgaagg cggctccgcc attcgcgtct
     5821 atccgctcga gaacgcgctc gcggaggagc tcgcggcaac cctgacgaac gtgctctcgg
     5881 gccaagggca gcagggccag cagcgcccgg gccagccgcc cgccgccgcg aacacgccac
     5941 gcccggccgg cgccgtcggc ggcgagttcg gcagctcgct cgaaggcacc gttcgcgtga
     6001 tcggcgacaa gccgacgaac tcgctgatcg tcatgtcgag cggccgtgac ttcatcgcgg
     6061 tgcgcgacgt gattcgccgg ctcgaccagc cacgccgcca gatcttcatc gaggcgctca
     6121 tcctcgaggt gcagctcaac aaagagctcg acatcggtac cagctcgcac ggcggtctgc
     6181 cggtcgcggg cggcgacgcg atcgtcctcg gtggcgtgca gacgccgacg ctgaagtcga
     6241 tcaacccgtc gtcgctggcc gcgctcacgg gcctcgtcgg tggcctcatc ggtacgccgc
     6301 tcgcgaactc gcagacgttc ctcggcacca gcatcccgtc gtacgccgtg ctgttccagg
     6361 cgctcgcgaa ccaggacaac tcgaacatcc tgtcggcgcc gcacatcatc gcgatcgaca
     6421 acgagaaggc cgagttctcc gtcggtaaca acatcccgta caaagcgggc ctgtcgttcg
     6481 gcggtcttcc cggcaccacg ggcgcgacga acccgctcgg ctcggtcggc acgaacatcc
     6541 agcgcgagaa gctgaacctg acgctcaacg tcacgccgca catctcgagc aacgactcgg
     6601 ttcgcctcga gatcgagcaa gagacgaagg acctcggcgg caaggacccc gagctcggcc
     6661 cgacgtgggc ggagcgcaag ctcaagacgc aggtcgtcgt ccacgatcaa gagagcgtcg
     6721 tgatcggcgg ccttatccag gaacgcgaga tctacaacgc gacgaaggtg ccgctcctcg
     6781 gcgacatccc gctgctcggc tacctcttca agtacacgac gaagcagaag aagaagacga
     6841 acctcctcat tcttctcacg ccgtacatca tcaaggacca gctcgatctg tcgacgatca
     6901 aagagcgcaa ggtccgcgag taccgcgagt tcgccgcctc gttcgcgaac ctcaacgaca
     6961 agaagtacga gccccgcatc gactatcgcc gcaagcgtgg tctgctcgag gagatcaacc
     7021 gcgccgtgca gtcggtggaa gaggatcaag ccgtgctgaa cgcgacgggc cagcgcatca
     7081 acgtcatgga aggcggcgtc gagttcggcc cgtcgagcat cgactcgccc gacgacgaca
     7141 agaagccaga cgcgcccaag accgacgcgc cgaagaccga cgccccgaag acggcaccgg
     7201 caccggagcc gaagaagtaa ccatggcgac gccgagctct tacatgttcc cgagcgatca
     7261 gagcgctggt ccggcgagga tcggcgagat cctcgtgcgc caatgcggcg tgtcgccgga
     7321 ctcgatcgag cgcgcgctcg gcaagcagcg cgaagagggc gggctgatcg gcgaggtgct
     7381 cgttcgcctc cgcctcatcg acgaagacca gctcgcgatg gcgctcgcga tccagcagga
     7441 catgacgtac ctgcgcgagc tgccgcgcgc cgaggacatt cccgccgagc taatcgacaa
     7501 gctgccgatc aacttcgcgc gccagcgtct catcctgccg ctcggtcgcg acggtgcgaa
     7561 ccgcgtgatg gtcgcgatcg ccgatccata caaggtcgag gtcatcgacg ccgtctccgt
     7621 gttgctcggc gagcccgtcg agcccgtggt cgcgtcggcg acgaagatca ccgaccacat
     7681 caacaaggtc tactcgagcc tgcgcggcgg tgccgagctc gaggcgggcg cgaagaagga
     7741 cgacgagggc gaagagttcg gtacgaacgc cgacgagctc gtcgacatgc tcgacgtcaa
     7801 cgacgaggcg ccgatcatca actgggtgaa cggcctggtg ttcgaggcgg cgaagaaggg
     7861 cgcgtccgat atccacatcc gtcccggcga gaaggacatc atcgttcgct accgcgtcga
     7921 cggcgtgctc cgcgaagtga agcgcgcgcc caagaagttc cagccatcga tcatcgctcg
     7981 cgtgaagatc atggccggtc tcaacatcgc cgagaagcgg ttgccgcaga gcggtcgcat
     8041 tcgccgcaag atggccggcc gcgatgtcga tatgcgtgtc gagaccgcgc cgactgcctc
     8101 tggtgagagc gtcacgatcc gtctcctcga tcgcagctcc gtgctcctcg gcctcgccga
     8161 catcggcttt gccgaagatc agctcaccca gatccgcagc atcatcgccc gcccgcacgg
     8221 catcttgctc gtcaccggtc cgacgggctc cggtaagacg acgacgctct acgcgtgcat
     8281 ctccgagatc aacgcgcctg acatcaacat cctcaccgtc gaggatcccg tcgagtatca
     8341 gctcgagggc atctcgcaga cgcaggtgaa ctcgaagatc gacctgacgt tcgcgtcggg
     8401 cctgcgctcg ttccttcgcc aagatcccga cgtcatcatg gtcggcgaaa ttcgtgaccg
     8461 cgagacggcg gaagtcgcga tcaccgcatc gctcacgggt cacctcgtgt tctcgaccgt
     8521 tcacacgaac gatgcggccg gcggcatcac gcgtctcgtc gatatgggca tcgagccgtt
     8581 cctcgtcgcg agctcgctcg tcggtctgct cgcgcagcgc ctcgtgcgtc gtccttgcta
     8641 cgagtgcgct cggccggtgc ggccatccga agagatcgtc aacgagctcg gcctcgatcc
     8701 ggcgctgttc ttccgtccgg gcggcggtta tcgcttccca gccgtgaagg gccagcgtcc
     8761 gcctccgccg ggaacggtgt tcgagcccgt cggatgtccg gcgtgccagc agctcggcta
     8821 caagggccga accggcatct acgagctcct gatgatcaac gagcccgttc gccgtctcgc
     8881 gctcgagaaa gccgacgcag gcgcgatccg caagagcgcc gaggaccacg gcatggtcag
     8941 cctgcgcggt gacggtgcac gcaaagtcgt cctcggaatg accacccctg aagaggtcat
     9001 gctgatgacc gcggagagca acgactgatg ccgatgtatg cgtacaaagg tgtcgcgaac
     9061 aacggcaaga ccgtgacggg cgtgcgtgat gcggactcgc cgaagacgct gcgccaagtc
     9121 atgcgcaaag acggcgtgct cgtcacgagc ttcgagctgt cgaagggcgg caaggcgcag
     9181 aaagagcagg cggcgaagaa gggcggcctc aatcgcgacg tcgacctcgg cggcctcatc
     9241 ggcggcgtcg gcaagacgga gatcgcggcg ttcacgcgcc agatggcgac gctgatccgc
     9301 tccggcattc cgctcgccga ttcgctcgcc gcgatggtcg agcagcagac gaacgttcgc
     9361 ttcaagacgc cgctctcgga agtgcgcgcg gccgtcaacg aaggctcgtc gttcgccgat
     9421 gcgctcgcga agcatccaaa gctgttcgac gagctattcg tctcgatggt tcgcgcaggc
     9481 gagctagccg gtaacctcga cgacgtactc ggccgtctcg ccgacttcct cgaggcctcg
     9541 cagaagctga agtcgaagat ccaaggcgcg atgatctatc ccgcggtcat ggtcgtcgtc
     9601 ggcaccggca tcatggccgt gctgatgatc aaggtcatcc cgcagatcac gtcgatgttc
     9661 acgtcgcagg gcaagacgct gccgctcaac acgcggtttt tgatctggac gtcgcggttc
     9721 atgggcaact actggctgct catcctgatc ggcatgatca tcgccggcat cctgttctcg
     9781 aagtggaagg gcagcaagga gggcaagccg gtgtggcatc ggttcgtcct caagctgccg
     9841 gtcctcggcc agctcgtccg cacgatcaac gtcggccgct tcgcccgcac gctcggcacg
     9901 atgctgcagt ccggcgtgcc gatgctgcgc gctctcgata ctgcgaagca gatcgtcggc
     9961 aacgtgctc
//
LOCUS       DATLBZ010000143         7115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1629074, whole genome shotgun sequence.
ACCESSION   DATLBZ010000143 DATLBZ010000000
VERSION     DATLBZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629074"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VMZ53_07665"
     CDS             <1..1167
                     /locus_tag="VMZ53_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HUS28368.1"
                     /translation="CIGNSGPVHDNIAAAVKDGNLVVASVLSGNRNFEGRVNPIVRAN
                     YLASPPLVVAYALSGNMNFDMETEPLGTGSDGKPVFLRDIWPTPAEVAELMRTSIKKE
                     QYQSRYAHVLEGDDEWRSLQIPAGDTFAWDDASTYIRKPSFFDNLGPTPKPLTDITGA
                     RVLAILGDSVTTDHISPAGNIAKGSPAARYLDDHGVSARDYNQYGARRGNHEVMVRGT
                     FANIRLKNLMLGGVEGGLTKHIPTNAQMSIYDAAMLYMQEGTPLIVIAGDQYGTGSSR
                     DWAAKGTMLLGVRAVIAKSFERIHRSNLIGMGVLPLQFEPGTDAATLGLTGNEVIEID
                     GIATGIIPKKKLVVKADGRTFNVIARVDTPQEVEYVVHGGILQYVLRQRAAVTK"
     gene            complement(1175..2521)
                     /locus_tag="VMZ53_07670"
     CDS             complement(1175..2521)
                     /locus_tag="VMZ53_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS28369.1"
                     /translation="MTDGRRGGTVIGLRKASTSELIPLSSGPQSWVVGSDASCDVRVH
                     NDPFVSGKHCVLERYADGMVIVRDNKSRNGTYVDGNHVEGAELRVGSYLTVGRTTLVA
                     VSDATARGGGNGAGAYAVERPAIELLRGKDAVLRMTVDQALRAAQTDCSVLIGGETGT
                     GKDLIARLVHESSRRMKGPFVAVNCGAIPRELIASELFGHEKGAFTGATEGRDGYFFE
                     AKGGTLFLDEIGELPIELQPTLLRVLETRRVRRVGGTTERHVDVRIVAATNRLDAIGD
                     EASGLRRDLYHRLATLTLVLPPLRERMGDLVELVEAMLEELAGDFGEKEVSEEAWLAL
                     ATYSWPGNIRELRHAVARAVTLGSRELTAADFFPHLNGRPRRLTLQVPSVEEAREDRG
                     AGTLVPYQAMLKTAMEQALHEHGSIRAAAMSLGMPKSTFADRAREWQLLGRRKRRA"
     gene            complement(2831..4117)
                     /locus_tag="VMZ53_07675"
     CDS             complement(2831..4117)
                     /locus_tag="VMZ53_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28370.1"
                     /translation="MKKLALGVLLAGLLVATGCGGDDHPHITLDAGSAATTCDPLKQT
                     GCAANEKCTWLLDALMPQYVGHIGCAPDGAANVGEQCVYGAPGTSGYDNCKKGTVCGN
                     YRGGMDVCKALCDQQGGMPECDATHVCVIYSKLFATSDTTPPAAGVCDLACDPFADND
                     FDGSGAMTKTGTTCGSGQGCYGYPSYGTPPKTGWSCTRDINNAEAQPIGLRHRVQCTE
                     DNNCADAGPTIYTNSCNQGYLPLLRESTTVSTTICVAICKPLSCYNGNCGTNDIKRKG
                     EGGNACTSNDRVAGASVMMTHDTAATHPNDEQCEFLWRREIDAMSGDYLPSETKDTVG
                     FCFDHSQYQYDSNGDNVADIDLPPCGALVQGFGSGSDLTDPLTYWGAADLGCVTSDEA
                     GVNMAQGKAKLPAGTLEKWRRVNMPRALYHRQANIR"
     gene            complement(4435..5295)
                     /locus_tag="VMZ53_07680"
     CDS             complement(4435..5295)
                     /locus_tag="VMZ53_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28371.1"
                     /translation="MARALVILVLLAFTAVAYADEPVGPQAIEEYIDRGKKLFEEQEY
                     AGAIQALAPVTRDVRATRAQRLRALEIIALAQFIRNDKGAARATFERILDIDPGYQLR
                     DTSGSPAMRAFFDDLKKQLIPGFDPKAGADLEHAAPTAGSAGTPVEVEVRVTRGNVFD
                     LVLATRRRGELTYALTPLAPRGENRWRAKITPPPSAKPYVLEYYVEARDAGGGPIARI
                     ANPNQPLEIALTAGGESAITPGRRAWYQHWYVYAGGAALAAGIGGVVVATTRGPDSGS
                     LPPHTITVTP"
     gene            complement(5280..6629)
                     /locus_tag="VMZ53_07685"
     CDS             complement(5280..6629)
                     /locus_tag="VMZ53_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HUS28372.1"
                     /translation="MNRAAAAAIVTLVWASGCTDAITLEVASNRPVPSAIDSVCVGVA
                     DESPSGGHFGQLYRLEDKLATLPQTLRVDAGNAERALAWARGDRGGVPVAYVSAHVDF
                     ANDVTLALDKCELGPSAAPAARGDAVGPANALVAASHGQGGTLVVAISTEGASVLAVV
                     DGRLAMSPTAAPALPAGTPIAIIAADVDSDCDDDLIVATNGAAPVVWLRDRAQLTAGM
                     SIGDVTVSAIAAADVDRDGDTDVVTGGGNRLELFRNDGSGQLASSPGLAAGSRATAIS
                     ALALGDLDGDGNPDLVVGQAGPPLVAWLGAEASFQPADGALPAVPLDVKRLALADADG
                     DFDPDLAVVVDGAPIRLYIDRDGRLEDQSFIRLPQNLPAANALFFGGWDAGCERDAII
                     ASDAGAPTLRGTPGGAFEAESSAPAPAAGDVVMTDIDDDGDLDAILATAQGVQWLAR"
     gene            complement(6753..>7115)
                     /locus_tag="VMZ53_07690"
     CDS             complement(6753..>7115)
                     /locus_tag="VMZ53_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS28373.1"
                     /translation="RVGTTDPQDRDSDFFVDGDWLATNRATAVDAQTEAAGVGTFTFQ
                     IKGPDVKTTTVYDEAFSFVQEGVTWFGPTFHVVLKVEPAIDDGGNGGCNAAGSSGGGL
                     GGGVLLALGLVLRRRRRC"
BASE COUNT         1173 a         2359 c         2402 g         1181 t
ORIGIN      
        1 tgcatcggca actcgggccc cgtgcacgac aacatcgctg cggccgtgaa ggacggcaat
       61 ctcgtcgtcg cgtcggtgct ctcgggcaac cgcaacttcg aaggccgcgt gaatccgatc
      121 gtgcgcgcga actacctcgc atcgccgccg cttgtggtcg cgtacgcgct gtcgggcaac
      181 atgaacttcg acatggagac ggagccactc ggcacgggct ccgacggcaa gcccgtcttc
      241 ctgcgcgaca tctggccaac gccggctgaa gtcgccgagc tgatgcgcac ctcgatcaag
      301 aaggagcagt atcaatcgcg ctacgcgcac gtgctcgagg gcgacgacga gtggcgctcg
      361 cttcaaatcc ctgccggcga cacgttcgcg tgggacgacg cgtcgacgta catccgcaag
      421 ccgtcgttct tcgacaacct cggcccgaca cccaagccgc tcactgacat cacgggcgct
      481 cgcgtgctcg cgatcctcgg tgactcggtg acgaccgatc acatttcgcc cgcgggcaac
      541 atcgcgaagg gctcgccggc ggcacgctac ctcgacgatc acggcgtgag cgcgcgggac
      601 tacaaccagt acggcgcgcg tcgcggcaat cacgaggtga tggtgcgcgg cacgttcgcg
      661 aacattcgcc tcaagaacct catgctgggt ggcgtcgaag gtggcctcac gaagcacatc
      721 ccgacgaacg cgcaaatgtc gatctacgac gcggcgatgc tctatatgca ggaaggcacg
      781 ccgctcatcg tgatcgcagg cgatcagtac ggcaccggca gctcacgcga ctgggcggcg
      841 aagggcacga tgctgctcgg cgtgcgcgca gtgatcgcga agagcttcga gcgcatccat
      901 cgctcgaacc tcatcggcat gggcgttctg ccactgcagt tcgagccggg caccgacgcc
      961 gcgacgctcg gcttgaccgg caacgaagtg atcgagatcg acggcatcgc gacgggcatc
     1021 atcccgaaga agaagctcgt ggtgaaagcc gacggcagga cgttcaacgt catcgctcgc
     1081 gtcgacacgc cgcaagaggt cgagtacgtc gtccacggcg gcatcctcca gtacgtcctg
     1141 cgccaacgcg cagctgtgac gaagtaggcc gctgctacgc acgccgcttc cgccgaccga
     1201 gtagctgcca ctcgcgcgcg cgatcggcga acgtcgactt cggcatgccg agcgacatcg
     1261 cagcggctcg aatggagccg tgctcgtgga gcgcctgctc catcgccgtc ttcagcatcg
     1321 cttgatacgg cacgagcgtg ccggcgccgc gatcttcgcg cgcctcttcg acgctcggca
     1381 cctgcagcgt cagtcgccgc ggccggccat tcagatgcgg aaagaaatcc gcggccgtca
     1441 gctcgcgact gccgagcgtc accgcgcgcg ccactgcatg ccggagctcg cgaatgttgc
     1501 cgggccacga gtacgtcgcg agcgcgagcc acgcctcctc gctgacctcc ttttcgccaa
     1561 agtctcccgc cagctcctcc aacatcgcct cgacgagctc gaccagatcg cccatgcgct
     1621 cgcgcagcgg cggcaacacg agggtcagcg tcgcaagccg atgatagagg tcgcggcgca
     1681 gccccgacgc ttcatcgccg atcgcatcga gccgattcgt cgcggcgacg atccgcacat
     1741 cgacgtgcct ctccgtcgtc ccgccgacgc ggcgcacgcg cctcgtctcg agcacgcgca
     1801 acagcgtcgg ctggagctcg atcggcagct cgccaatctc atccaagaac agcgtgccgc
     1861 ccttcgcttc gaagaagtag ccatcgcgtc cctcggtcgc gcccgtaaac gcgcccttct
     1921 cgtggccaaa cagctccgac gcaatcagct cgcgcggaat cgcgccgcag ttcacggcga
     1981 cgaacggccc cttcatgcgg cgcgagctct cgtgcacgag tcgcgcgatc aagtccttgc
     2041 ccgtccccgt ctcgccgccg atcagcacgc tgcaatccgt ctgcgccgcg cgcaacgcct
     2101 gatccaccgt catgcgcagc accgcatcct tccctcgcag cagctcgatc gcaggccgct
     2161 cgacggcata cgccccggcc ccattgccgc cgccgcgcgc cgtcgcatcc gaaaccgcga
     2221 ccaacgtcgt gcgccccacc gtcaaatacg atccaacgcg cagctccgcg ccctcgacat
     2281 gattcccgtc gacgtacgtc ccattgcgcg acttgttatc gcgcacgatg accatcccat
     2341 ccgcatatcg ctcgagcacg cagtgcttgc ccgaaacaaa tggatcgttg tgcacgcgca
     2401 cgtcgcagct cgcgtcgctg cccaccaccc agctctgcgg cccgctgctc agcggaatga
     2461 gctcgctcgt ggatgcctta cgaagaccga tgaccgttcc accgcgccgg ccatctgtca
     2521 ttttgtgagt cgtcgttggt tcgaaagtcg tcatgatcgc cacccctggt aatcgtgtct
     2581 gcgcgttatc ggtttcgtcg tcggacagtt gcgtccgatg ttcgtgccga ccgcagtgcg
     2641 gccgatgtgc cgcacgcaac cacgcaatac ctctcgcggg agtgtccgga gtgtccggcg
     2701 cccggcgagg acagacgtca ccacgcgctc cgaaaacgaa acgagccgga caacgccggc
     2761 tctgcatcgc acgaacgccg aaaatgaaaa gaggccggac gcgccggcct ctttacgaac
     2821 aacgactcgc ttagcggatg ttcgcctggc ggtggtagag cgcgcgcggc atgttcacgc
     2881 ggcgccactt ctcgagcgta ccggccggca gcttcgcctt gccctgggcc atgttgacgc
     2941 cggcttcgtc cgacgtgacg cagccaaggt cagcagcgcc ccagtacgtg agcggatccg
     3001 tcaggtccga gcccgagccg aagccctgga cgagcgcacc acacggcggc agatcgatat
     3061 cggcgacgtt gtcaccgttc gagtcgtact gatactgcga gtgatcgaag cagaagccga
     3121 ccgtgtcctt cgtctcggaa ggcaggtagt cacccgacat ggcgtcgatc tcgcggcgcc
     3181 acaggaactc gcactgctca tcgttcggat gggtcgcggc ggtgtcgtgg gtcatcatca
     3241 cgctggcgcc tgcaacgcgg tcgttgctgg tgcacgcgtt gccgccttca cccttgcgct
     3301 tgatgtcgtt cgtgccgcag ttgccgttgt agcaggacag cggcttgcag atcgcgacgc
     3361 agatcgtcgt cgacaccgtg gtgctctcgc ggaggagagg caggtaaccc tggttgcagc
     3421 tgttcgtgta gatcgtcggg cctgcgtccg cgcagttgtt gtcttccgtg cactggacgc
     3481 gatggcggag accgatcggc tgcgcttccg cgttgttgat gtcgcgcgtg cagctccagc
     3541 cggtcttcgg cggcgtgccg tagctcgggt agccatagca gccttggccc gaaccgcagg
     3601 tcgtgccggt cttcgtcatg gcgccgctgc cgtcgaagtc gttgtcggcg aacgggtcgc
     3661 acgcgaggtc gcacacgcca gccgcgggcg gcgtcgtgtc actcgtcgcg aacagcttcg
     3721 agtagatcac gcacacgtgg gtggcgtcgc actcgggcat gccgccctgc tgatcgcaga
     3781 gggctttgca gacgtccatg ccgccgcggt agttgccgca gaccgtgccc ttcttgcagt
     3841 tgtcgtagcc ggaggtgccc ggcgcgccgt agacgcattg ttcgcccacg ttcgcggcac
     3901 cgtcaggtgc gcaaccgatg tggccgacgt attgcggcat cagcgcgtcg agaagccagg
     3961 tgcatttctc gttcgccgcg caacccgtct gcttgagtgg gtcacacgtc gtcgctgccg
     4021 aaccggcgtc cagcgtgatg tgagggtgat cgtcgccgcc gcatccggtc gcaacgagga
     4081 gccctgcgag cagaactccc aacgctagct tcttcatttc cgcaattcct ctcatcgatt
     4141 gctgcgtcgg ggcgcaattg tcccggacag cgcagctcca aagttggtgg accctatcgg
     4201 gtggaaccat gggggtcaag tccatttcct gactgggatc acggcccgga tcccggcatt
     4261 ggagaacgat cggatgcgga cggttttccg acgatgatgg aagtcacggt cgggtgctac
     4321 gagggctaac aatggggcgg cgactgaggt gcccggtctc ggagtcagcg acgcacgcag
     4381 acgcaccttg tcgctgcggg cgaagggctt gagaagtcgt ccgattttcg ggcttcaggg
     4441 tgtgacggtg atcgtgtgcg gtggcaacga gccggagtct gggccgcggg ttgtggcaac
     4501 cacgacaccg ccgatgcctg cggcgagggc tgcgccaccg gcgtagacgt accaatgctg
     4561 gtaccaggcg cggcggccgg gggtgatcgc ggactcgccg cccgcggtga gggcgatctc
     4621 gagaggctgg ttgggattgg cgatgcgagc gatcgggccg ccacctgcgt cgcgcgcttc
     4681 gacgtagtac tcgagcacgt agggtttggc ggagggaggt ggcgtgatct tcgcgcgcca
     4741 gcgattttcg ccgcgcggtg cgagcggcgt gagtgcgtag gtcagctcgc cgcggcggcg
     4801 cgtggcgagg acgagatcga acacgttgcc gcgcgtgacg cgcacttcga cttcgaccgg
     4861 cgtgcctgcg gagccggcgg tgggcgcggc atgctcgagg tcggcgcccg cctttggatc
     4921 gaagccgggg atgagctgct tcttgagatc gtcgaagaac gcgcgcattg cgggcgagcc
     4981 cgacgtgtcg cgaagttggt agccaggatc gatgtcgagg atgcgctcga acgtcgcgcg
     5041 cgcggccccc ttgtcgttgc ggatgaattg agcgagagcg atgatctcga gagcgcgaag
     5101 gcgttgtgcg cgggttgcgc ggacgtcgcg cgtgaccggc gcgagcgctt ggatcgctcc
     5161 tgcgtactct tgttcttcga agagcttctt gccgcgatcg atgtattcct cgatcgcctg
     5221 cgggccgacc ggctcgtcgg cgtatgcgac ggcggtgaac gcgagcagca cgaggatgac
     5281 tagcgcgcga gccactggac tccttgtgcg gtcgcgagga tcgcgtcgag gtcaccgtcg
     5341 tcgtcgatgt cggtcatgac gacgtcgcct gcggccgggg ctggggcgct ggattcggcc
     5401 tcgaaggcgc cacccggtgt tccgcgcagc gtcggtgcac cggcatcgga ggcgatgatg
     5461 gcgtcgcgtt cgcagccggc gtcccagccg ccgaagaaga gcgcgttcgc cgccggtagg
     5521 ttttgcggca ggcgaatgaa ggactggtct tcgaggcggc catcgcgatc gatgtagagg
     5581 cgaatgggcg cgccatcgac gacgaccgcg aggtcgggat cgaaatctcc gtcggcgtcg
     5641 gcgagcgcga ggcgtttgac gtcgaggggg acggcgggga gtgcaccgtc ggcgggctgg
     5701 aagctcgctt cggcgccgag ccacgcgacg agcggcgggc ccgcttggcc gacgacgaga
     5761 tcgggattgc cgtcgccgtc gaggtcgccg agcgcgagcg cgctgatggc ggtggcgcgc
     5821 gagcccgcgg cgaggccggg cgatgaggcg agctggccac tgccgtcgtt gcgaaatagc
     5881 tcgaggcggt tgccgccgcc cgtgacgacg tcggtgtcgc catcgcgatc gacatcggcg
     5941 gctgcgattg cggagacggt gacatcgccg atcgacatgc cggccgtcag ctgcgcgcga
     6001 tcgcgaagcc acacgacagg tgcagcgccg ttcgtcgcga cgatgagatc gtcgtcgcag
     6061 tcgctgtcga cgtcggctgc gatgatcgcg atcggtgtgc ctgcagggag cgcgggcgca
     6121 gcggtcggcg acatcgcgag gcggccgtcg acgacggcga ggacgcttgc gccctccgtg
     6181 gagatcgcga cgacgagcgt gccgccctgc ccgtgcgacg cagcgacgag cgcgttggcg
     6241 ggaccgacgg catcgccgcg cgccgcgggc gccgctgagg gaccgagctc gcatttgtcg
     6301 agcgcgagcg tgacgtcatt cgcgaagtcg acgtgcgccg aaacgtatgc gacgggaacg
     6361 ccaccgcgat cgccgcgcgc ccatgcgagc gcacgctccg cattgcctgc gtcgacgcgc
     6421 agcgtttggg gcagcgtcgc gagcttgtcc tccaggcgat agagctggcc gaagtggccg
     6481 ccgctcggcg actcatcggc gacaccgacg cagacggagt cgatcgcgct tggcaccgga
     6541 cggttgctcg cgacctcgag cgtgatcgca tcggtgcagc cgctcgccca gacgagcgtc
     6601 acgatcgctg cagccgcggc gcggttcacg cggcgagcat acacactcgc gacgcagttc
     6661 gcgtcagcga cgacgacgcg agaaagcgga agacggtcgc gcgaacgctt gcgacgacga
     6721 cgacggaaag ccggtgcgag gtcgcgggat cgttagcaac gacggcgacg acgcagcacg
     6781 aggccgagcg cgagcaggac gccgccgccg aggccaccgc ccgacgagcc cgccgcgttg
     6841 cagccgccgt tgccgccatc atcgatcgcg ggctcgacct tgagcacgac gtggaacgtc
     6901 ggcccgaacc aggtcacgcc ttcctgcacg aacgagaatg cctcgtcgta gacggtcgtc
     6961 gtcttcacgt cggggccttt gatctggaac gtgaacgtgc cgacgccagc ggcttcggtc
     7021 tgcgcgtcga ccgcggtcgc acgattcgtc gcgagccaat caccgtcgac gaagaagtcg
     7081 gaatcgcgat cttgtggatc cgtcgtgccg acgcg
//
LOCUS       DATLBZ010000144         4435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_883635, whole genome shotgun sequence.
ACCESSION   DATLBZ010000144 DATLBZ010000000
VERSION     DATLBZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883635"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VMZ53_07695"
     CDS             complement(<1..406)
                     /locus_tag="VMZ53_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28374.1"
                     /translation="MRSRRAWIYVLSVLALLVPAGGIAYLGAFSYADDRGAVSAQNQR
                     QQQVAAAIAERIATQIDAAFTEIDLALQRKDVPRGPLSAPLAHYWFWIDPEGHMRVPR
                     APAPAVFDDAALERAPCGGRLEDCVRESTTRQV"
     gene            445..1161
                     /locus_tag="VMZ53_07700"
     CDS             445..1161
                     /locus_tag="VMZ53_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HUS28375.1"
                     /translation="MAVALIIVPTYNEAENVRGIAERLLEALPVADVLFVDDNSPDGT
                     GAMIDEMVAENPRVHVMHRAGKLGVGTAYVEGFAWGLARDYKYIFEMDADGSHDPKYL
                     PQMLALAEDGADAVVGSRNIPGGGTVNWGVGRQLLSKGGSLYARTILGIDVRDVTAGF
                     VCWRREVLENLDLPTVDSNGYSFQIEMKYRALKRGYRVVETPIVFVDRRVGQSKMSRA
                     IVAEALLKVWALRFRGGGRG"
     gene            1207..1827
                     /locus_tag="VMZ53_07705"
     CDS             1207..1827
                     /locus_tag="VMZ53_07705"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS28376.1"
                     /translation="MNILRLRIKSADEWKELAPSESVSEPIFVPTTDELNAGEQVIVE
                     ISSNLLPNKVLIRGSVSAWRPALPRMRVRAGATIQLDADEQVKLTFLHDVLSGKRTDV
                     PRRKNSRLPVQFACRYRVAESSQFIDSAISEIGVGGALLTTPTPLPLETELTIEVTPP
                     GAAAPIAIAGRVSYHAPSGGSGLRFVSRDGDGDRRLRELIRRLRAS"
     gene            1840..2364
                     /locus_tag="VMZ53_07710"
     CDS             1840..2364
                     /locus_tag="VMZ53_07710"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HUS28377.1"
                     /translation="MARHWNTGPRDKFDRAKGMVAVRDLIKNVVNYYRLNADIRQEML
                     FAEWSSFVGDRIGARTRPDTIIDRTLIVEVATSAWLHELRLIRPKIVSDMLDKLGLPR
                     FFDDIRFVLAGERRRRGPEVRRPAPRKKAEAAPPPPMPAVGEDLRKILDDTSKIEDEE
                     LRALVSRVRVKNDR"
     gene            complement(2348..2806)
                     /locus_tag="VMZ53_07715"
     CDS             complement(2348..2806)
                     /locus_tag="VMZ53_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28378.1"
                     /translation="MAANRALIPNPALQPLKFLVGEWTLRGSHPAVPGVTLHGHSSVV
                     WLDGGAFLRMESAIDDKRFPNGIAIIGSDGDELSMLYFDERKVSRHYRVAVTETGWRW
                     WRDDDKLAQRFTVTLEDGGNTMAGKGEMSRDGGAWEGDLDLTYTRASGRS"
     gene            complement(2812..>4435)
                     /locus_tag="VMZ53_07720"
     CDS             complement(2812..>4435)
                     /locus_tag="VMZ53_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HUS28379.1"
                     /translation="RVGILFMMLAIGAYLVWKNLGGDSEKGGQELFVRMKDASALPTG
                     SKVVVAGLRKGEVTKLEVRGRYAVVYFTLSKDIPIWSNAVVYKKASSLLGENYLEIDP
                     GEPFQQKPDGSKLAFTPLGNASTPDHPKCADYDSSNDQKRWACREIPHMVEAVTPDEL
                     IHRIEATLPRVDTVLESVHDLSEDVRRIVNGPMKNVADRVDGLVQKQAQTVESIIERA
                     DRMMANVEQLTKDVRAITADADPKVAKILADLEQASAEAKDLVATAKQELKDTGDAVR
                     GKLDKLDGVMTASESIAKKIDEDKGTLGRLVNDPAIADNVESITEDAKGFLGTLFGLK
                     AYVGLRSEWNFRAGLARHYVSVELHTRPDKFYLVELEKGPRGNYPDVSLVFDPTVDPN
                     NWIKKTVIEDKIRFTFQFAKRFGWLTLRYGIKESTGGIGADADIPWMNRNIRLSADVF
                     DATFDKYPRAKLTAAMEVFKHVYVLGGVDELLNDSQTLPIIAGNSEVPVQFEEFRFGR
                     DYFLGGMLRFTDEDLAALLTVGGSAIAGAGGK"
BASE COUNT          728 a         1454 c         1449 g          804 t
ORIGIN      
        1 tcacctggcg cgtcgtcgat tcgcgcacgc agtcttcgag ccgaccgccg cacggtgcgc
       61 gttcgagcgc cgcatcgtcg aacaccgcag gtgcgggcgc gcgaggcacg cgcatgtggc
      121 cctctggatc gatccagaac cagtaatgcg cgagcggtgc cgagagcggc ccgcgtggga
      181 cgtctttgcg ctgcagcgcg agatcgatct cggtgaatgc tgcatcgatc tgtgtcgcga
      241 tgcgctcggc gatcgctgcg gcgacctgct gctgccgctg attctgcgcg gacaccgcgc
      301 cacgatcgtc ggcgtacgag aacgcaccga gatatgcgat gccgccggcc ggcaccagca
      361 atgccagcac cgagagcacg tagatccacg cgcgccggga tcgcacccca agcagcatag
      421 cgcgtactat cctgcgcgac ctacatggcc gtcgcgctca tcatcgtgcc cacttacaac
      481 gaggccgaga acgtgcgcgg catcgcggag cggttgctcg aagcgttgcc cgtcgccgac
      541 gtcctgttcg tcgatgacaa ctcgcccgac ggaaccggcg ccatgatcga cgagatggtc
      601 gcggagaacc cgcgcgtcca cgtcatgcat cgcgctggca agctcggcgt cggcaccgcc
      661 tacgtcgagg gcttcgcgtg gggcctcgcg cgcgactaca agtacatctt cgagatggat
      721 gccgatggca gccacgatcc gaagtatctg ccgcagatgc tcgcgctcgc cgaagacggc
      781 gccgatgcag tcgtcgggtc gcgcaacatc ccaggcggag gcacggtcaa ctggggcgtc
      841 ggccggcagc tactctcgaa aggcggcagc ctgtacgccc gaacgatcct cggcatcgat
      901 gtccgcgacg tgacggcggg cttcgtctgc tggcgccgcg aggtgctcga gaacctcgac
      961 ctcccgaccg tcgattccaa cggctacagc ttccagatcg agatgaagta ccgcgcgctg
     1021 aagcggggct accgcgtcgt ggaaacccct atcgttttcg tcgaccggcg ggttggccag
     1081 tcgaagatgt cgcgcgcgat cgtggctgaa gcgctcctga aggtctgggc cctgcgattt
     1141 cgcggcggtg gccgcggttg aagtggcgtt catgcacgat cctttgctac cgtcgtcgcg
     1201 gttgcgatga acatcctgcg gctccgcatc aagtccgccg acgagtggaa ggagctcgct
     1261 ccgagtgaga gcgtctccga acccattttc gtgccgacca cggacgagct gaacgcgggc
     1321 gaacaggtca tcgtggagat ctcctccaac ctgcttccga acaaagtcct catccgcggc
     1381 tcggtctcgg cctggcgccc cgcccttccc cgcatgcgcg tgcgcgctgg cgcgacgatc
     1441 cagctcgacg ccgacgagca agtgaagctg acgttcctcc acgacgtgct gtccggcaag
     1501 cgcaccgacg tcccgcgccg caagaacagc cgcctccccg tccagttcgc gtgccgctac
     1561 cgcgtggccg agtcgtcgca gttcatcgac agcgcgatct cggaaatcgg agtcggcggc
     1621 gcgctgctca cgactccaac gccgctaccg ctcgagaccg agctgacgat cgaagtcacg
     1681 cctcccggcg ccgctgcacc gatcgcgatc gcgggccgcg tctcgtatca cgcaccgtcg
     1741 ggcggctcgg gcctgcgctt cgtctcgcgc gacggtgatg gcgaccgccg cctgcgcgag
     1801 ctgatccgcc gcctgcgcgc atcgtaggtc agtcacacca tggcgcggca ctggaacacc
     1861 ggtccccgcg acaagttcga tcgcgcaaaa ggcatggtcg cggtccgcga tctgatcaag
     1921 aacgtcgtca actactaccg gctcaacgca gacatccgcc aagagatgtt gttcgcggag
     1981 tggtcgtcct tcgtcggtga cagaatcggc gcgcgcacgc gaccggacac gatcatcgat
     2041 cgcacgttga tcgtcgaagt cgcgacctcg gcgtggctgc acgagctgcg cctcatccgc
     2101 ccgaagatcg tgagcgacat gctcgacaag ctcggcctgc cgcgcttctt cgacgacatc
     2161 cgcttcgtcc tcgccggcga acgccgtcgc cgcggccccg aagtgcgccg ccctgctccg
     2221 cgcaagaaag ccgaggcggc accgccgcca ccgatgcccg cggttggtga agacctgcgc
     2281 aagatcctcg acgacacgtc gaagatcgaa gacgaagagc tgcgcgcgct cgtgtcgcga
     2341 gttcgcgtca agaacgaccg ctagctcgcg tatacgtcag gtcaaggtcg ccttcccacg
     2401 cgccgccgtc cctcgacatc tcgcccttgc cggccatcgt attgccgccg tcttcgagcg
     2461 tcacggtgaa gcgctgtgcg agcttgtcgt cgtcgcgcca ccagcgccag ccggtctcgg
     2521 tcaccgcgac gcggtagtga cgcgacacct tgcgctcgtc gaagtacagc atcgagagct
     2581 cgtcaccgtc gctgccgatg atcgcgatgc cgttcggaaa ccgcttgtcg tcgatggccg
     2641 attccatgcg caggaatgcg ccaccgtcga gccacaccac ggacgagtgg ccgtggagcg
     2701 tcacgccggg aacagccgga tgcgagccac gcaaggtcca ctcgccgacg aggaacttca
     2761 gcggttgtag cgcgggattc gggatcaacg ctcggttcgc ggccatgccg gctatttacc
     2821 gccggcgccc gcgattgccg agccgcccac cgtgaggagc gcagcgagat cttcgtccgt
     2881 aaagcgcagc atgccgccga ggaagtagtc gcggccgaag cggaactctt cgaattggac
     2941 gggcacttcg gagttgcccg cgatgattgg cagcgtctgc gagtcgttga gcagctcgtc
     3001 gacgccgccg agcacgtaca cgtgcttgaa gacttccatc gcagcggtga gcttcgcgcg
     3061 ggggtatttg tcgaacgttg cgtcgaacac atcggcggag aggcgaatgt tgcgattcat
     3121 ccacgggatg tctgcgtcgg cgccaatgcc gcccgtcgac tccttgatgc cgtagcgcag
     3181 cgtcagccag ccgaagcgtt tcgcgaactg gaacgtgaag cggatcttgt cctcgatgac
     3241 ggtcttcttg atccagttgt tcggatcgac cgtgggatcg aagacgagcg agacgtctgg
     3301 gtagtttccg cgcgggccct tctcgagctc gacgagatag aacttgtcgg ggcgcgtgtg
     3361 gagctcgacg gagacgtagt ggcgtgcgag gccggcgcgg aagttccatt cgctgcggag
     3421 gccgacgtat gccttgaggc cgaacagcgt gccgaggaag cctttcgcgt cttcggtgat
     3481 cgattcgacg ttgtcggcga tcgcggggtc gttgacgagg cggccgagcg tgcctttgtc
     3541 ctcgtcgatc ttctttgcga tcgactcgct ggcggtcatt acgccgtcga gcttgtcgag
     3601 cttgccgcgc acggcgtcgc ccgtgtcttt gagctcttgc ttcgcggtgg ctacgaggtc
     3661 tttcgcctcg gcggaggctt gctcgaggtc ggccaggatc ttggcgactt tcggatcggc
     3721 atcggcggtg atcgcgcgga cgtctttcgt gagctgctcg acgttcgcca tcatgcgatc
     3781 ggcgcgctcg atgatcgact cgacggtttg cgcctgcttt tgcacgaggc cgtcgacgcg
     3841 atcggcgacg ttcttcatcg gcccgttgac gatgcggcgc acgtcttcgg agaggtcgtg
     3901 cacggactcg agcacggtgt cgacgcgcgg cagcgtggct tcgatgcgat ggatcagctc
     3961 gtcgggcgtg acggcctcga ccatgtgcgg gatttcgcgg caggcccagc gcttctgatc
     4021 gttgctcgag tcgtagtcgg cgcacttcgg atggtcgggc gtcgacgcgt tgccgagtgg
     4081 cgtgaacgcg agcttgctgc cgtcgggctt ttgctggaac ggctcgcctg gatcgatctc
     4141 gagatagttc tcgccgagca gcgagctcgc cttcttgtag acgaccgcgt tcgaccagat
     4201 cgggatgtcc ttcgacagcg tgaagtagac gacggcgtag cggccgcgca cttcgagctt
     4261 ggtgacttcg cctttgcgaa ggcccgcgac gacgaccttg ctgccggtgg gcagcgccga
     4321 cgcgtccttc atgcggacga acagctcttg gccgcccttc tcggaatcac cgccgaggtt
     4381 cttccagacg aggtacgcac cgatggccag catcatgaag aggatgccga cgcgc
//
LOCUS       DATLBZ010000145         2840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9074549, whole genome shotgun sequence.
ACCESSION   DATLBZ010000145 DATLBZ010000000
VERSION     DATLBZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9074549"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VMZ53_07725"
     CDS             complement(<1..344)
                     /locus_tag="VMZ53_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28380.1"
                     /translation="MRRGLLLGLLCAACGDGGTEAIQNPARDSYETWIKVEPPGVVCG
                     NNTPYKFFVNFSAKSDNLVVVLEPGGACWDYDSCTGANGIRGAANVNGLQDDHFKLAP
                     FISPFLNRSSDEV"
     gene            complement(415..2094)
                     /locus_tag="VMZ53_07730"
     CDS             complement(415..2094)
                     /locus_tag="VMZ53_07730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28381.1"
                     /translation="MGEPAEEPRGDETPPTAHALPANAIARRAAAASASKDAPAPHVS
                     QQIALRTLREEQADAAVQMIRVGRLLAIASILPLPFVGGNDVLRVALAASLVIAIAIG
                     AIAERHIRKSVFSERLTLLLGLSVSPAIFMAVLYFGILSAVQLFPALALHFFSRRESK
                     GAAFALYLLNSGMQALFATLIVAGVVKDPGLVTPHLPTGTLIAGFALLQVAFLGAFLL
                     GRGAHQASRDAIEKMQKAMTLAAQREALLQEARADLDRALAIDAAGRFTEHTFGQYRL
                     GNVIGRGGMGEVYEAFHVDSGAPAAVKLLAKSELGNPQSVERFLRELRAIRGLESKHV
                     VRVLASSGERDEIPYLVMEKLAGHDLAHHLRSGRMSPASLLEMLDQVGSAVEEAWAKG
                     IVHRDLKPQNLFLADGVGWKVLDFGIAALEESSGTLTQGKVVGTPAYMAPEQARGEKV
                     DHRADVYALAAIAYRWLTGRPAVSGKDLHNALYQTVHVMPQKPSVLAELHEDVDRALA
                     IGLVKEPGDRYDRVSELRDALAKALAGELDPRLRRRAADLLGRHPWGAVRG"
     gene            complement(2111..>2840)
                     /locus_tag="VMZ53_07735"
     CDS             complement(2111..>2840)
                     /locus_tag="VMZ53_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28382.1"
                     /translation="WALLLLLTACGRLGFDSRSDASTDGTTPLDVMLVADAQQPWTGP
                     VPIAGGWTNLPGNAATISTPPLQFAAGDFIVVAVGWRSAQATISSITSSTGITYTQRT
                     PIEGPGTVTQVMYTSVASMNAPETVFVDFGGAVPSPSIRILSYRNATMSVPTGSGATG
                     NGALATTGVVTTNRADVMLVAALSAASPATGPGVGFTIQCLTNPSGDVFQDMRAPSPA
                     SYQGTAPLTASAGYIGHMLMLEPP"
BASE COUNT          510 a          949 c          928 g          453 t
ORIGIN      
        1 acttcgtcgg agctgcggtt caagaacggc gagatgaacg gcgcgagctt gaagtgatcg
       61 tcctgcagac cgttgacgtt cgccgcgccg cggatgccgt tggcacccgt gcagctgtcg
      121 tagtcccagc acgcgccacc cggctcgagc acgacgacga ggttgtcgct cttggcgctg
      181 aagttgacga agaacttgta cggcgtgttg ttgccacaca cgacgcccgg cggctcgacc
      241 ttgatccagg tctcgtatga atcgcgcgct ggattctgga tcgcctccgt cccgccatcg
      301 ccgcacgccg cgcagagcag tcccaacaac agtcctcgcc tcatgggagc gaacgctaca
      361 cgagagacga ttggcccgac cagcaaagaa tcagctgagc gcgctacaga tcactcaacc
      421 acgcaccgcg ccccatggat ggcggccgag cagatcggcg gcacgtcgac gaagacgcgg
      481 gtccagctcg cccgcaagcg cctttgcgag cgcatcgcga agctcgctga cacggtcgta
      541 gcgatcgccg ggctccttca cgagaccgat tgcgagcgcg cggtcgacgt cctcgtgcag
      601 ctccgcgagc acgctcggtt tctgcggcat gacgtggacg gtctgataga gcgcgttgtg
      661 gaggtccttg cccgagacgg cggggcgccc ggtgagccag cgatacgcga tcgcggcgag
      721 cgcgtagacg tcggcgcgat gatcgacctt ctcgccgcgc gcttgctcgg gcgccatgta
      781 cgccggtgtg ccgacgacct tgccctgcgt cagcgtgcca gagctttcct cgagcgcggc
      841 gatgccgaag tcgagcacct tccagccaac gccatcggcg aggaacaagt tctgcggttt
      901 gagatcgcga tgcacgatgc ccttggccca cgcctcttcg acggcggagc cgacttgatc
      961 gagcatctcg aggagcgacg cgggagacat gcgcccgctt cgcagatggt gcgcgagatc
     1021 gtggccggcg agcttctcca tcacgagata gggaatctcg tcacgctcgc cgctcgatgc
     1081 gaggacgcgt acgacgtgct tgctctcgag cccacgaatc gcgcgcagct cgcgcaaaaa
     1141 tctctcgaca ctctgcggat tgccgagctc gctcttcgcg aggagcttca cggcagcagg
     1201 tgcgccgctg tcgacgtgaa acgcttcgta cacctcgccc atcccgccgc gaccgatcac
     1261 gttgccgagc cggtactgcc cgaacgtatg ctcggtgaaa cgaccggcgg catcgatcgc
     1321 gagtgcgcga tctagatcgg cgcgcgcctc ctgcaacagc gcttcacgtt gcgccgcgag
     1381 cgtcatcgcc ttctgcatct tctcgatcgc atcgcgcgac gcttgatgcg cgccacggcc
     1441 gagcagaaac gcgccgagga atgcaacttg gagcagcgca aagcccgcga tcagcgttcc
     1501 cgtcggcaga tgcggcgtaa cgaggcctgg atctttcacg acgccagcga cgatcagcgt
     1561 cgcaaacagc gcctgcatgc ccgagttgag cagatacagc gcgaacgccg cgcctttgct
     1621 ctcgcggcgg ctgaagaagt gcagcgccag ggcagggaag agctgcaccg ccgacaggat
     1681 gccgaagtac agcaccgcca tgaagatcgc aggcgacacc gagagcccga gcagaagtgt
     1741 caggcgctcc gagaacaccg acttgcgaat gtggcgttcc gcgatagcac cgatggcaat
     1801 tgcgatgacc agtgaggcgg cgagcgcaac gcggaggaca tcattgccgc cgacgaacgg
     1861 cagcggcagg atcgatgcga tcgcgagcaa acgtccgacg cggatcatct gcaccgcggc
     1921 gtcggcttgc tcctctcgca gcgtgcgcag cgcgatctgc tggctcacgt gcggggcggg
     1981 cgcgtccttc gatgcagacg ctgccgcagc gcgacgcgcg atcgcattcg ccggcaacgc
     2041 atgcgcggtc ggcggcgtct catcgccgcg tggctcctca gccggttccc ccacgcccca
     2101 tcgtaaccgc tcagggtggt tcgagcatca gcatgtggcc gatgtacccg gcggacgcgg
     2161 tcagcggagc tgttccctga tacgacgcgg gcgacggcgc tcgcatgtcc tggaacacgt
     2221 cgccggacgg attcgtcaga cattggatcg tgaagcccac accggggccc gtcgcgggcg
     2281 acgcagcgga gagcgcagcg acgagcatga cgtcagcccg attcgtcgtc acgacgcccg
     2341 tcgtcgcgag cgcaccattg cccgtggccc ctgagcccgt aggtacagac atcgtcgcgt
     2401 tgcgatagct caggatccga atcgacggcg atggaaccgc gcccccgaag tcgacgaaga
     2461 ccgtctcggg cgcgttcatc gatgcgaccg acgtgtacat gacctgcgtg accgtgcccg
     2521 gtccttcgat cggcgttcgt tgcgtgtacg tgattcccgt cgagctggtg atcgacgaaa
     2581 tcgtcgcctg cgcagaacgc cagccgaccg ccacgacgat gaagtcgccg gcagcgaact
     2641 gcaacggggg cgtcgagatg gtcgcggcgt ttcccggtag attcgtccat ccgccggcga
     2701 tcggaaccgg accggtccac ggctgctgcg cgtcggccac gagcatcaca tcgagcggcg
     2761 tcgtgccgtc ggtgctcgcg tcgctgcgac tgtcgaagcc gaggcgtccg cacgcggtca
     2821 gcagcaggag cagcgcccag
//
LOCUS       DATLBZ010000146        20597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_883714, whole genome shotgun sequence.
ACCESSION   DATLBZ010000146 DATLBZ010000000
VERSION     DATLBZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20597
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883714"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..2693
                     /locus_tag="VMZ53_07740"
     CDS             60..2693
                     /locus_tag="VMZ53_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS28383.1"
                     /translation="MRAIFRRREVEAELDDELQFHLEQSIEANVRAGHSPEEARRLAR
                     IELGGVASVKEGVRDARGVRLLFDLGADLRYGLRGLRRNPLFAAVAIITLALGIGATT
                     AMFAIVDDVLLHPVPYEQPDRLVRLHASKPAFDKGSVSYPNFLDWQAANHSFESIAAS
                     RTAAFTLTGTGTAERVSADLISPDYFKVLRVAPIIGHTFGAEENVALLGEALWRRKFG
                     ASRDVVGSKIILDGDAYTVAGVMRASADLRGVAGGRGIDLYVPITQLSRDALKQRGAG
                     LSIHGIARLAPGVTLPQARADMDAVAASLAEQYPETNRGVGAAIEPLADSIVGNVRPY
                     VIVLFAAVALVLLIACVNVANLLLARATVRSHELAIRVAVGASFGRLVRQLLTESLLL
                     AIGGGALGLLVAWWSTDVLLAILPGRLSRVHVDSFDPRLLLFTIGVSVFAGVLSGLVP
                     ALKAIRPNLYDTLKQGGRTSTARSGAQSIFVIAQTAMAVVLLVGAGLLVRTMVRLAAV
                     DPGYEPDHLVSFGVSLAPTLQAAPPGVARAKLDELREGLAASPGVTAVSFAFGSVPIE
                     GSDQSNFWVDGRPKPATSHEMAWTVTSIVDEHYIETMGIQLQRGRFFSTRDDASAPNV
                     IVIDDAFAQLHFPGEDPVGKRIHLADYDFEPVEIVGVVRHVKMWGLDQDDTTTVRAQV
                     YIPFRQLGDPMVMRAAAGVIVLARTDASSAASVTSLHSSVERLGSDNVMFRIRNADEI
                     IESYQATRRFAMYVLAAFAVLALILCCVGIYGVISYMVARKTTEIGVRMALGATASMI
                     VRLVLRDGLRLAFSGILLGLVTACFVTRFMADLLFGVHPIDPVTFGVVASTVTLVAVV
                     ALLIPARRATKLNVTDALRTE"
     gene            2728..3384
                     /locus_tag="VMZ53_07745"
     CDS             2728..3384
                     /locus_tag="VMZ53_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HUS28384.1"
                     /translation="MAAQAFTRNYQDHSNDTGYQFEFNCDKCGNGYRSTFQASALGVG
                     SKIAKGLGSFFGGSKLWGAGVAADYMKDGLRGSGWDDAFKKAIEEIKPKFHQCTRCGQ
                     WVCPEVCWNEQRGLCEGCAPDLAEEAAHHQAQIASQQIANKMHNADLIGNVDVTAQML
                     SGCPHCKARLAPGAKFCAGCGKPVVQGAGQATKFCSGCGSQLQGGARFCAGCGTPAAT
                     "
     gene            complement(4103..4444)
                     /locus_tag="VMZ53_07750"
     CDS             complement(4103..4444)
                     /locus_tag="VMZ53_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28385.1"
                     /translation="MSPIANQIYKHLLSHVRKNKGAITYAELAKAVDVHARSPKLHSA
                     LGEVANACRHAQLPVLPAIVCRADSKRPSDGYYKVAHPRARTDEARVAAWQREHAKVL
                     GAVGDFPPKLA"
     gene            complement(4441..6330)
                     /locus_tag="VMZ53_07755"
     CDS             complement(4441..6330)
                     /locus_tag="VMZ53_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HUS28386.1"
                     /translation="MVALIKSCTLVGIDAVPIDVECVISSGQLPSFNIVGLATASVKE
                     GAVRVKSALLSIDHELPIKRVTVNLAPADLRKPGCALDLPTALSILIADGIYDGSVLD
                     GLLVLGELGLDGSVRGVPGVLAAAMLARDKGMRGVIVPDRSAAEALVVEDIEVYGVSH
                     LDQVVAALAGEAELPLPSKAERRRRVRRRRVADMSDVRGQAFARSAIEVAVAGGHNLL
                     LAGPPGTGKTMLARRLPGVLPPMTREESLETTKIYSALGLSDGLIEERPFRAPHHTIS
                     TAALLGGGTTPRPGEISLAHTGVLFLDELPEFARSTIEALRQPLEERAMTIGRVHGTI
                     CLPASFLLVAAANPCPCGWHGFKERECTCSNAAIERYRARLSGPLLDRIDLQVPVKPI
                     TLGQLRDETPGEPTAIMRDRVSLARARQAARFAGTPIRTNAEMSSAALREHCKLDDAS
                     EATLASIVEKHGSMSARGINRLLKVALTIADLCGSDRINPPILQEASTYRAVDPTADG
                     SLALGGPETVKRKKSRLFRGPLEETANAAPPSTDGDANATSASNDTDVIAASHSTDAG
                     ANASSHSTDGHANAASHSTEGGPNAPASPSPDRVADAPRVHMPSPSILLPIAPDTTHT
                     ESQLS"
     gene            6461..7240
                     /locus_tag="VMZ53_07760"
     CDS             6461..7240
                     /locus_tag="VMZ53_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS28387.1"
                     /translation="MRSKTSPQIAFDDVGDREPALLALPGWCAQRTAFRPMYSHLERR
                     VLAVDWRGHGGSEAVTGEFGVAELVEDALAVMSAAGAERVIPLATAHAGWVAIELRRR
                     LGADRVPGVVLVDWMVLGAPPPFMSGLAALQNVEQWQAMRDRLFAMWTEGVSSPEVQA
                     FVRTMGHFGFDMWSRAGREIANAFAAQPVPLEALAPLECPTLHVYAQPADPAFLAAQE
                     DFARKHPWFRVQRVSAHSHFPTIEAAAEVAAAVESFARDLA"
     gene            7352..7897
                     /locus_tag="VMZ53_07765"
     CDS             7352..7897
                     /locus_tag="VMZ53_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HUS28388.1"
                     /translation="MICLALGLGVLGFIAARKARRCGRGGGCGGYGRGGWHGHGGGWH
                     GHGGWPGWRGHHGRRGLYMALSHLDASPAQERAIIAEVDKLREKLWSTKRVVKDGRGD
                     LAAAIRASELDDAALGAVLGRVDGATADARAAILEALRNIHAVLDDKQRAQLADILEG
                     GFWGGRGGHRGGPHGGHPYRV"
     gene            7910..8602
                     /locus_tag="VMZ53_07770"
     CDS             7910..8602
                     /locus_tag="VMZ53_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS28389.1"
                     /translation="MRVLLIDDDARLAELLVGYLQPQGVTLVHAGGGQAGLGQLASGG
                     FDAVLLDVMMPGMDGLAVLRKVRDAGHRVPVIMLTARGDEADRVVGLELGADDYVAKP
                     FSPRELLARLRAVLRRAAPDSVAEKLTAAGITVDVGAREAFVDGKPVELTGLEIDLLA
                     ALLRRAGRVVPRSALMELAGRGDVAVNERAVDVHISRLRKKLGDESAARIRTVRGVGY
                     TLAKSLDEEPSS"
     gene            8599..10278
                     /locus_tag="VMZ53_07775"
     CDS             8599..10278
                     /locus_tag="VMZ53_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS28390.1"
                     /translation="MSARSSDSEPLKLRVVMGSIERAGAWTVPPEVRAHVFWGNLELD
                     LRDADIGDVTTIDAHVTMGNLQIVVPREMIVDVAVNSFAGNVENRQPSSPRQVPPTLD
                     GSGERTFPGHAYRDPVRAPAKRLRVTGSVKFANCEIIPLDPGETLHDHARRVREDLRR
                     RRHYLLGPHDPHHRVVVPGPGGPGPGGPGMHGPEQMPGGFGHGVPSHGPFSHRGHHRH
                     HHRRRGRNREERLAALEAEREIWRAHPDRSAWLPWWLQARMRRRIFSWFAIAFVLGTL
                     AGIYLWPDHRWWHFVLAALGLSVMSGAVSWRLTRPLILVVQAARDIGDGKLDTRLDIH
                     QRGEMKILAMAINDMASRIEQQLKDQKQLLAAVSHELRTPLGHMRVLIETARDTHDAK
                     ALSELDKEVLTLDDLVGRLLASSRLEFGNLDKRTIDLGELASDLATQAGVAPEGIEAE
                     GDVKAAVDPTLVRRAIANLLDNAKTHGGGAVAVRVVRRGPQVVVEVDDAGPGVGKDRR
                     ADAFRAFVPSSGGGLGLGLALVSRIAVAHEGGAYIHDRPGGGARVGFTVTA"
     gene            complement(10262..10945)
                     /locus_tag="VMZ53_07780"
     CDS             complement(10262..10945)
                     /locus_tag="VMZ53_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28391.1"
                     /translation="MSESEKEWSMRRMAMLLAGCTLGVLVLMVITGVITGATQEKHEH
                     FMAPEEYALSLLAEPKGLRTLMGLDIAFAILYTAFFAAFTKYLRALGRPFVTLAFGAM
                     LATAILDFIEDHHIISMLESAEHRVFPTASAIQFQAAESAVKFSMSFLALVLYGLAVP
                     RTTKLGLALCLFLVVGTLISGVLGYSAPPEFQAKLEGGRWIGFLIGLGLAIAWLRSAP
                     EPAAATPSP"
     gene            complement(10988..11668)
                     /locus_tag="VMZ53_07785"
     CDS             complement(10988..11668)
                     /locus_tag="VMZ53_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28392.1"
                     /translation="MLTNPLIAAAGGAVWGVSAAIDAIRRSRARAKRKRIQSVIGGMP
                     DLDDIRDADTRATVAKMQQARQNLQKVIDDTPADVLMQLQKTLDSLIQLELYAASLVK
                     RAEDTTAYLRSVNLPSLVQEVKLLATKAAQAKDPEARASFDQAKNARMEQIRALKELR
                     TSKERVDANLMRVVAVLGALPTKIVRLRALDAQAMEVLSGDITHDLTEMGEELKTSEQ
                     VIRQLVPQ"
     gene            11831..12604
                     /locus_tag="VMZ53_07790"
     CDS             11831..12604
                     /locus_tag="VMZ53_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HUS28393.1"
                     /translation="MILGKFFGAIGAQFNKLGNWLFNKDPIAQMQYEYDKAVEQLKEG
                     RVGLEQYRGLVERVSRQVKEGDATVTKLTSQAKAYLKMGNREMAAQFAPQLTKAKAQL
                     AENQQQLQMHEEAYNNNLKKIQHANKKLIELKDKIHKYDADLKMSEAEAEIAKISSSF
                     NMDVTTNFGQLEDNIQRRIDTNRGRARVAADMSQQGIENIKAEEQLEKSMADDALKDL
                     EVELGMRAPETTPVQQAQKDLGPAEAEAARKELENLEKS"
     gene            12608..14404
                     /locus_tag="VMZ53_07795"
     CDS             12608..14404
                     /locus_tag="VMZ53_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS28394.1"
                     /translation="MADDPKTLAGSVDGVTKATQLPAHFPAWAAKLAELYFSGTTSMF
                     VLHGNTFDVVRAGPENDPRWTGLADFLADQLFGRWDLVIHYDLARGLRCAAGTSSKRL
                     VEMVATSNKWIGDLKALPRDPTKGLLALDLMIQKIVMADEDKRIRCAIIIDHVGYVAP
                     SGDNLDLSEQTHLVTLLNWASSPYIKRLNMAFVLIDPRLSSIAERLSSNPHVAQIEVP
                     LPNADERTAFIAAQVKSTGKEIATFSDYSVPELGKLTAGIGLTDLEVLVRSAAEGTRR
                     LDRQYFKELKKRLIERQAQGMLEFVEPKWGLDTVVGHEAAKKRLLDDAELLKRGELDT
                     VPMGYLFCGPVGTGKSFLATCVAGSIGIPAVVLKNFRSKYVGETEGNLERVLGVMRSM
                     GPVVVVIDEADAMLGDRDSGGDSGVGGRVFAMIASQMGDTAYRGRIIWMLLTARPDQL
                     PVDLKRQGRAEVHIPLFYPTDQAELKTMLMVLAKKSGTSLAESDLPDTVPHMGNLSGA
                     DIEGIIGRAYRTSLLGGSKTITKAAIEQALAGFMPSTQTLEREAQELAAIIECTDVEF
                     LPQPKRDKLDKFGGREKLQERLTAIKQILEER"
     gene            14407..14691
                     /locus_tag="VMZ53_07800"
     CDS             14407..14691
                     /locus_tag="VMZ53_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28395.1"
                     /translation="MARTSWIDADSNPDLDAHVSKLEHFTASLADGVITSDELEKQEN
                     NLVSAMKSVEASLNDEQHAKVTKLLAELTAYSVMRTLHDLTKAHVKGLKL"
     gene            14692..15441
                     /locus_tag="VMZ53_07805"
     CDS             14692..15441
                     /locus_tag="VMZ53_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28396.1"
                     /translation="MRRGCLRVAAIVSTVAACGRVAFETRIDAEAPTPDALVVDAPTG
                     VTFVRNIAAQMFTANGSAASIPLQASAAGNAILVHATCTSVTTPMVATLEMTGTWTFT
                     QLGPVERYLSQQGVVLGAIAPNTNPTSLLVGFSIPDCAPLSIVADELTGNDPTGGTTT
                     FDANAQGIGSGGCSVPLVTGSDDETLWGACISEFPITGVGTEFSKAGDDGNDHWSEYR
                     VGTGPAGTSVATSFMNGVGGYLAAGVAIKPR"
     gene            complement(15445..15660)
                     /locus_tag="VMZ53_07810"
     CDS             complement(15445..15660)
                     /locus_tag="VMZ53_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28397.1"
                     /translation="MSNNNRDDDLAGLLKRAADAAASEGIPSEAFMTVAWQAFLAAHP
                     GMREELEDKALRTELKKLRKRGLIASA"
     gene            complement(15724..15945)
                     /locus_tag="VMZ53_07815"
     CDS             complement(15724..15945)
                     /locus_tag="VMZ53_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28398.1"
                     /translation="MPIPNDDDNLELQRLLRCAAQLATAHSVESEQFLVAACQAFLAC
                     NPALRAKLEEEREVLDMAAARGRGKVAQA"
     gene            complement(16040..17071)
                     /locus_tag="VMZ53_07820"
     CDS             complement(16040..17071)
                     /locus_tag="VMZ53_07820"
                     /inference="COORDINATES: protein motif:HMM:NF022932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HUS28399.1"
                     /translation="MRLSSALPLFVLLAGACADTSDPASTPPDGAGGKQDSAEPIEDF
                     CNATDPRAMPVEVAVTPDAGEQPYLDALTSAQHSIDVEIYLMGYGGILDTLVAKAHAG
                     VKVRVILDQYKMDTNQKYFTQLAAAGAEVKWSSAEFTYQHSKVLVVDGTTAVISTGNY
                     SKSYSIELERNHVATDRDPADLADLVALFEADWTGQPVQMPCTRMVISPINSRTRILD
                     LINSATQTLTIESMQFADYKVREAVKARVQAGVDVRALIADAGFVSANAAAADYLKGL
                     GVTVKWIPHLHTKVIVADGARAYLGSENLSQTSLDKNREVGLIVTDAASIAPLSTSFE
                     TDWTAGTAF"
     gene            complement(17147..17530)
                     /locus_tag="VMZ53_07825"
     CDS             complement(17147..17530)
                     /locus_tag="VMZ53_07825"
                     /inference="COORDINATES: protein motif:HMM:NF022703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HUS28400.1"
                     /translation="MRKLLLAVALLAPSTALADKDLSEPTDGTTWDCANDATVNVNYG
                     EATFTFTGECKEINVNGSTVQITLDSVETLNVNGASNVIKTTALHAANINGTKNKVTY
                     KKVLGKGKKPKVASVGKGNAVTKVK"
     gene            complement(17584..19419)
                     /locus_tag="VMZ53_07830"
     CDS             complement(17584..19419)
                     /locus_tag="VMZ53_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUS28401.1"
                     /translation="MAKDLDHQLWRGQRPWFEIWFAVLLDENRRRALWLRESLFIPKE
                     GDGRATIWGAWFDADAMPKTRAGKRILPLDQAITGEGEQLIRIADSHVARTGVVGGID
                     GLTWKATWSGGKPVRDEVPTWLPAPTHARELAHDADCEATIEVDGKPIALRGKAFAMH
                     LWGKKRVPTLHWVWTPWLGNGSLEVTAVSLRDTFSLGISSLSLDGRDGITGTPATAAH
                     PHGLVTSTIAGTRRLVHAHAWADADELVGYAYRDTDGRDLMVAQSDIGSAYYESFQRT
                     APGLPWKLVEERRVAGGVAVEIHQRAKLPGVSYIEWDDASAAPRIDRTNIASDSVDWP
                     PVESIVALGLTYADHLKETGQTHDAGTPPATFAKHLRSFIANSLEQNIHVAVPTYDQL
                     LAALADVEPGLDAKLRDRIARMPAVMDYEGELALVALGDIDEDALTAGTPQPLGLAAA
                     NDLTARICQVFGEGTQTPLAYWACAKSFPRFLPVAPRVWAPKGGFAKLPELELVTRVN
                     GEERQRGTTRDLLYDLPAIVRAARQQLGRPLGRGDVILTGTPAGVGMKLSWLDRKLAR
                     LVSDRFRKAEYLISTYATSNALMRPGDVVEVDAGPAGRVRARLTV"
     gene            19539..20111
                     /locus_tag="VMZ53_07835"
     CDS             19539..20111
                     /locus_tag="VMZ53_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS28402.1"
                     /translation="MASRTEDRGRLLSELAGAPARKGYGIAYDLLGNRAEAEEAVQEA
                     LARACESIADLRDPVAAPAWFLRIVTTMCLRTLRRRKLKRALFGWMPGKDDSEPKPDA
                     STDAIAERMHGPTGQAPVEALTDREEFGALMSRLDQLSAQQRTALVLRYGHDLGVPEI
                     AELLGVELATAKTHLVRGLARLRDLMEEHR"
     gene            20108..>20597
                     /locus_tag="VMZ53_07840"
     CDS             20108..>20597
                     /locus_tag="VMZ53_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28403.1"
                     /translation="MTTCDVVAERVALGEPLGDAADHAERCPKCRRLVALPTELGATH
                     READPGLGFAARMTAGAQHRVVVRRRRRIAAGMAGVVAAAAMAAFFVTREPDAPKLAT
                     TPPDQTQQPATNPHEANKNDPWDASASSDVDEDVRALIQLADTDAASRTTAHWKKIEQ
                     PLD"
BASE COUNT         3446 a         6539 c         7032 g         3580 t
ORIGIN      
        1 gacgagcttg tcgcgttcgt gtcggaggac gcgcgatgtt cgctgatctg cgttaccggg
       61 tgcgggcgat ctttcggcgg cgcgaggtcg aggcggagct cgacgacgag ctgcagtttc
      121 acctcgagca gtcgatcgag gcgaacgtgc gggcggggca ttcgcccgag gaggcgcggc
      181 ggctcgcgcg catcgagctc ggcggcgtgg cgtcggtgaa ggagggcgtg cgcgacgcga
      241 gaggcgtacg gctgctgttc gatctcgggg cggatctgcg atacgggttg cgcgggttgc
      301 ggcgcaatcc gttgttcgcg gccgtcgcga tcatcacgct ggcgttgggc atcggcgcga
      361 cgacggcgat gttcgcgatc gtcgacgacg tcttgctgca tccggtgccg tacgagcaac
      421 ccgatcggct cgtgcgcttg catgcgagca agccagcctt cgacaagggc tccgtctcgt
      481 atcccaactt tctcgattgg caggcggcga atcactcgtt cgagtcgatc gcagcgtcgc
      541 gcacggcggc gtttacgctc accgggaccg gcaccgcgga gcgcgtgtcg gcggatttga
      601 tttcgccgga ttacttcaag gtgctgcgcg tggcgccgat catcgggcat acgttcggcg
      661 cggaggagaa cgttgcgctg ctcggcgagg cgctgtggcg caggaagttc ggcgcatcgc
      721 gcgacgtggt gggatcgaag atcatcctcg acggagatgc gtacaccgtc gctggcgtga
      781 tgcgggcgag cgcggatctg cgcggagtgg cgggcgggcg gggcatcgat ttgtatgtac
      841 cgatcacgca gctcagccgt gacgcgttga agcagcgcgg tgcggggctg agcattcatg
      901 gcatcgcgcg gctcgcgccc ggtgtcacgc ttccgcaggc gcgtgctgat atggatgcgg
      961 tcgcggcgag cctcgccgag caatatcccg agacgaaccg cggcgtcggg gcggcgatcg
     1021 agccattggc ggactcgatc gtcggcaacg tgcggccgta tgtgatcgtc ctgttcgctg
     1081 cggtcgcgct cgtgctgttg atcgcctgcg tgaatgtcgc gaacttgctg ctcgcgcgcg
     1141 cgacggtgcg gtcccacgag ctcgcgattc gcgtcgccgt cggtgcgagc ttcggcaggc
     1201 tcgtgcgaca gctgctcacc gagagcctct tgctcgcgat tggcggcggt gcgctcggcc
     1261 tcctcgtcgc gtggtggagc accgacgtcc tactggcgat tctgcctggc cggctctcgc
     1321 gcgtgcatgt cgacagcttc gatccgcggc tgctcctgtt cacgatcggc gtgtcggtct
     1381 tcgcgggcgt cttgtccggc ctcgttcccg cgctcaaagc aatccggccg aacctgtacg
     1441 acacgctcaa gcaaggcggc cgtacctcga cggcgcggag cggcgcgcag tccatcttcg
     1501 tgatcgcgca gactgcgatg gcggtcgtgc tgctcgtcgg cgcgggcttg ctggtccgga
     1561 cgatggtgcg gctcgcggcg gtcgatcccg gttacgagcc cgatcacctc gtgtcgttcg
     1621 gcgtctcgct cgcaccgacg ctgcaagccg cgccgccagg agtcgcacgc gcgaagctcg
     1681 acgagctgcg tgaaggcctc gcggcctcgc cgggtgtcac cgccgtgtcg ttcgcattcg
     1741 gcagcgtgcc gatcgaaggc agcgatcagt cgaacttctg ggtcgatggc cgtccgaaac
     1801 cggcgacgtc gcacgagatg gcgtggaccg tgacgtcgat cgtcgacgag cactacatcg
     1861 agacgatggg catccagctg cagcgtggcc gcttcttctc gacgcgcgac gacgccagcg
     1921 cgccgaacgt catcgtgatc gacgacgcct tcgcgcagct tcactttccc ggcgaggatc
     1981 cagtcggcaa gcgaattcat ctcgccgatt acgacttcga gccggtcgag atcgtcggcg
     2041 tcgtgcgcca cgtcaagatg tggggcctcg atcaggacga cacgacgaca gtccgcgcgc
     2101 aggtctacat cccatttcgc caactcggcg atcccatggt catgcgcgca gccgccggcg
     2161 tcatcgtgct cgcgcgaacc gatgcgagca gcgccgcgtc ggtgacctcg ctgcactcga
     2221 gcgtcgagcg cctcggcagt gacaacgtga tgtttcgcat ccgcaacgcc gacgagatca
     2281 tcgagagcta ccaggcgacg cggcgcttcg cgatgtacgt gctcgcggcc tttgccgtgc
     2341 tcgcgctcat cctctgctgc gtcggcatct acggcgtcat ctcgtacatg gtcgctagga
     2401 aaacgaccga gatcggcgta cggatggcgc tcggcgcgac ggcgtccatg atcgtgcgac
     2461 tcgtcctgcg cgacggcctc cgcctggcgt tcagcggaat cctgctcggt ctcgtaaccg
     2521 catgcttcgt gacccggttc atggccgacc tcctgttcgg agtgcacccc atcgacccgg
     2581 ttacgtttgg ggtagtcgcg agcacggtaa cgctcgtagc cgtggtggca ctgctcatcc
     2641 cggcaagacg cgcgacgaag ctgaacgtaa cggacgcgct ccgaacggag tgaagcgcgg
     2701 tcgcagaacg agtaaagtag ccgtccgatg gctgcccaag cgttcacccg taactaccaa
     2761 gatcacagca acgacactgg ctatcagttc gagttcaatt gcgacaagtg cggcaatggc
     2821 taccgctcga cgttccaagc gagcgcgctc ggcgtcggct cgaagatcgc aaagggcctc
     2881 ggctctttct tcggcggtag caagctctgg ggtgcgggcg tggcggcgga ctacatgaag
     2941 gacggccttc gcggctccgg ctgggacgac gccttcaaaa aggcgatcga agagatcaag
     3001 ccgaagttcc accagtgcac gcgctgtggc caatgggtct gccccgaagt ctgctggaac
     3061 gagcagcgcg gcctctgcga agggtgcgcg cccgacctcg ccgaagaagc ggcgcatcac
     3121 caagcgcaga tcgcgtcgca gcagatcgcg aacaagatgc acaacgccga tctgatcggc
     3181 aacgtcgacg tcaccgccca gatgctttcg ggctgcccgc actgcaaagc gcgtctcgcc
     3241 ccaggcgcca agttctgcgc tggttgcggc aagccggtcg tccaaggcgc cggccaagca
     3301 accaagttct gctcgggctg cggctcgcag ctccaaggtg gcgcccgatt ctgcgcaggt
     3361 tgcggcacgc ccgcggctac ctgacgcaga cgtagctagc cccgatcagc ttcgccggac
     3421 gcgagctcga cgaacccaac cggggtcgac gtacctcgac tagcaacgtg tcgcccatga
     3481 tcgagtacac gatgcgatag gaccaccgcc tccgtcggat agtcacggtc ttcgtgaatg
     3541 agtcgggaat ggtcgggaga aacggaattc cgctacgagg cgcgtgcgcg agaacgagta
     3601 gcgcggcgta gagcttttct cgtgcgacgc gcggaaccct ttcgagctga cgcgctgccc
     3661 gcggcgtgag cacgatcgtc aacatcgacg tcgtgcttcc gcggtgcatc ctccgccggg
     3721 tggtcgtcgt gcttccgccg gaactgctcg agcgtgatgc aacgtccttc ggccaggtcg
     3781 ttgcgactct gcgccatctc tgttcgccag tccggatgcg cagtcatgaa atcgatcgtc
     3841 gcgatgagtg actcgatgta ctcgctgtcg acgaccagca gcggacgatg tccacgcctg
     3901 gtgattcgat agacggcgcc agacgcctcg atgtccgtcg caatgcgttc ggcacgcctc
     3961 gacagatcgc tcatcgagat cgtcttgtac ttcggcatct cgtcgagaat aggaacaatc
     4021 ttcgagcacg gcaatcgaaa cctccgggcg cggacatgcg atcgcgcgca gtgcgttgct
     4081 ccagtcgcga gctcgaggtg ggctacgcga gcttgggagg aaagtcgccg acggccccga
     4141 ggaccttcgc gtgctcgcgc tgccacgcag cgacgcgagc ttcgtcggtg cgcgcgcgcg
     4201 ggtgcgcgac cttgtagtag ccatcgctcg gccgcttgga atcagcgcga cacacgatcg
     4261 caggcagcac cggaagctgc gcgtggcggc acgcgttcgc gacttcgccg agtgcggagt
     4321 gcagcttcgg cgagcgcgca tggacatcca cggctttcgc gagctcggcg tacgtgatcg
     4381 cgcccttgtt cttacggacg tgagagagga ggtgcttgta gatctggttt gcgatcgggc
     4441 tcatgacagt tggctttcgg tgtgagtggt gtcgggtgca atcggaagga ggatggaggg
     4501 cgatggcatg tggacacgtg gcgcatcggc gacacggtct ggagagggcg acgccggagc
     4561 gttcgggcca ccctcggtcg aatgcgacgc ggcgttggca tgaccgtcgg tcgaatgcga
     4621 cgaggcgttg gcaccagcgt cggtcgagtg cgacgcggcg atcacgtcag tgtcgttcga
     4681 cgccgatgtg gcgttcgcgt caccgtcggt cgaaggcggc gcggcgttgg cggtctcttc
     4741 gagtgggccg cgaaacagcc tcgacttctt gcgcttgacc gtctcgggcc cacccagggc
     4801 gagcgagccg tcagcggttg gatcgaccgc gcggtacgtc gatgcctcct ggaggattgg
     4861 cgggttgatt cgatcgctgc cgcaaaggtc ggcgatcgtg agcgcgacct tgagcagacg
     4921 attgatgccg cgcgcgctca tcgagccgtg cttctcgacg atcgacgcaa gggtggcctc
     4981 gctcgcgtcg tcgagcttgc agtgctcgcg cagcgcggcc gagctcatct cggcgttcgt
     5041 gcgaatgggg gtgcctgcga agcgcgcagc ctgacgcgca cgtgcgagcg acacgcgatc
     5101 gcgcatgatc gcggttggtt caccgggtgt ctcgtcgcgc agctgtccga gcgtgatcgg
     5161 cttgacgggt acttgaagat cgatgcggtc gagcaaagga ccgctgaggc gcgcgcggta
     5221 tctctcgatg gcggcgttgc tgcacgtgca ttcgcgttcc ttgaagccat gccaaccgca
     5281 cggacacggg ttcgcggccg caaccaacaa gaaggacgcg ggcagacaga tcgtcccgtg
     5341 gacgcgaccg atcgtcatcg cgcgctcctc gagaggttgc cgaagcgcct cgatcgtcga
     5401 tcgcgcaaac tcgggtagct cgtcgaggaa taagacgccc gtatgagcga gagagatctc
     5461 accgggccgt ggcgtagtac cgccaccgag gagcgctgcc gtgctgatcg tgtggtgcgg
     5521 cgcgcgaaag ggccgctctt ctatgaggcc gtccgataac ccgagagccg agtagatctt
     5581 cgtggtctcg agcgactcct cgcgcgtcat cggcggcagt acgccgggaa gccggcgcgc
     5641 gagcattgtc ttgcccgtcc ctggcggacc cgcgagcaac aggttgtggc cgccggcgac
     5701 ggcaacttcg atcgcggagc gcgcgaacgc ttggccgcgc acgtccgaca tgtccgcgac
     5761 ccgccggcgc cggacacgcc ggcgacgctc cgccttggat gggaggggca actccgcctc
     5821 gcctgcgagt gcggcgacca cctgatcgag atgcgagacg ccgtagacct cgatgtcctc
     5881 gacaaccagc gcctccgccg cggagcgatc ggggacgatc acgccacgca tacccttgtc
     5941 tcgcgcgagc atcgcggcgg cgaggacacc gggaacgccg cgaaccgaac catcgaggcc
     6001 cagctcgccg aggacgagga gcccgtcgag cacgctgccg tcgtagatac cgtcggcgat
     6061 caggatcgag agcgcggtcg gcagatcgag cgcgcagccg ggcttgcgca agtcggcggg
     6121 cgcgaggttg accgtcacgc gtttgatcgg cagctcgtga tcgatcgaga ggagcgcgct
     6181 cttcacgcgc accgcgcctt cttttaccga ggcagttgcg agaccgacga tgttgaacga
     6241 cggaagctga ccggacgaga tcacgcactc gacgtcgatc ggaactgcat cgatgccaac
     6301 gagtgtgcag gacttgatga gggcgaccat gcggcggcag agagcacgtc atgggccaac
     6361 gatcgtgata tgggttgtcg cgaggttacg agtttggtcg tccggtagcc ggacgtcgtg
     6421 gccgggtggc gttcggacac cggacaaagg ggaaagcacg atgaggtcga agacgagccc
     6481 gcagattgcg ttcgatgatg ttggtgatcg cgagccggcg ctgttggcgt tgccaggatg
     6541 gtgcgcgcag cggacggcct ttcgaccgat gtattcgcat ctcgagcgga gagtgctcgc
     6601 ggttgattgg cgtgggcatg gcggctcgga ggcggtgacg ggtgagtttg gcgtcgccga
     6661 gctcgtcgaa gatgcgcttg ccgtgatgtc ggcggcgggc gcggagcgcg tcattcctct
     6721 ggcgactgcg catgcgggat gggttgccat cgagctgcgg aggcgacttg gcgcggaccg
     6781 cgtgcccggt gtcgtgctcg tcgattggat ggtccttggt gcgccgcctc cctttatgag
     6841 tgggctcgcg gcgctgcaga acgtcgagca gtggcaggcc atgcgcgatc ggttgttcgc
     6901 gatgtggacc gagggcgtca gctcgccaga ggttcaggcg tttgtcagaa cgatgggcca
     6961 cttcggcttc gacatgtggt cgcgcgctgg gcgcgagatc gcgaatgcat ttgcggcgca
     7021 gcctgttccg ctcgaggcgc tcgcgccgct cgagtgcccg acattgcatg tctacgcgca
     7081 gcccgctgat cccgcgtttc tggccgcaca ggaagacttt gctcgcaagc acccgtggtt
     7141 ccgcgtgcaa cgcgtttctg cgcactcgca ttttccgacg atcgaagcgg ctgccgaggt
     7201 cgctgcggcc gtcgagagct tcgcgcgcga cttggcgtag cggacatctc agcggcggcg
     7261 cggacttacg cgctgcaagg tttattcaca caacacctgc cagttccgaa acacggggtc
     7321 gccatcctgg cttggtgaaa ggaacctgaa aatgatttgt ctcgcactcg gacttggcgt
     7381 cctcggattt attgcggctc gtaaggctcg tcgatgtggt cgcggcggag gttgcggtgg
     7441 gtatggacgc ggcggctggc atggccacgg cgggggctgg catgggcacg gcgggtggcc
     7501 cggttggcgc ggtcatcacg gccggcgtgg gctctacatg gcgctctcgc atttggatgc
     7561 gtcgcctgcg caggagaggg cgatcatcgc ggaggtcgac aagctccgcg agaagctgtg
     7621 gtcgacgaag cgcgtcgtga aggacggccg cggtgatttg gcggcggcga ttcgcgcgag
     7681 cgagctcgat gatgcggcgc tcggcgcggt gctcggtcgc gtggatggtg cgaccgcgga
     7741 tgcgcgcgcg gcgatcctgg aggcgctccg caacattcat gcggtgctcg acgacaagca
     7801 gcgcgcgcag ctcgccgaca tcttggaggg aggcttctgg ggtggacgcg gtggccatcg
     7861 cggcggcccg cacggcggcc atccctaccg cgtgtgagag actatggacg tgcgcgtgct
     7921 gctcatcgat gacgacgccc gcctcgcgga gctgctggtt ggctatctgc aaccgcaggg
     7981 cgtcacgctc gtgcatgccg gtggtgggca ggcgggcttg gggcagctcg cgagcggcgg
     8041 cttcgatgcg gtgttgctcg acgtgatgat gcccggcatg gatgggctcg cggtgctgcg
     8101 gaaggtccgc gacgctgggc atcgggtgcc cgtcatcatg ctgaccgcgc gcggcgatga
     8161 agcggatcgc gttgtcgggc tcgagctcgg tgcggacgat tatgtcgcca agccattttc
     8221 gccgcgcgag ctcctcgcgc gtctgcgggc ggtgttgcgg cgagcggccc cggactcggt
     8281 tgccgagaag ctgacggcgg cgggcattac cgtcgatgtc ggcgcgcgcg aggcgtttgt
     8341 cgacggcaag cctgtcgagc tgacgggatt ggagatcgac ttgctggcag cgttgctgcg
     8401 tcgagcgggt cgcgtcgtgc cgcgcagtgc gctcatggag ctggcgggac gcggcgatgt
     8461 tgcggtcaac gagcgcgcag tcgatgtcca catctcgcgg ttgcgcaaga agctcggcga
     8521 cgagagcgcg gcccgcattc gcaccgtgcg cggcgttggc tacacgctcg cgaagagcct
     8581 cgacgaggag ccgtcatcat gagcgcgcgg agcagtgaca gcgagccatt gaagctgcgc
     8641 gtcgtgatgg gctcgatcga gcgcgccggc gcgtggacag ttccgccgga ggttcgcgcg
     8701 cacgtgttct ggggcaacct cgagctcgat cttcgcgacg ccgacatcgg cgacgtaacg
     8761 acgatcgacg cgcacgtcac gatgggcaat ctccagatcg tcgtgccgcg cgagatgatc
     8821 gtcgacgtcg cggtgaattc gttcgccggc aacgtcgaga accggcaacc gtcgagcccg
     8881 cgccaagtgc cgccgacgct ggacggaagc ggcgagcgaa cctttccagg gcacgcatat
     8941 cgcgatcccg ttcgcgcgcc cgcgaagcgc ctccgcgtga ccggctccgt gaagttcgcg
     9001 aactgcgaga tcatcccgct cgatcctgga gagacgctcc acgatcacgc gcgccgcgtg
     9061 cgcgaagatc tgcggcggcg gcgtcactac ttgctcggcc cgcacgatcc gcatcatcgc
     9121 gtcgtcgttc cgggtcctgg cggtcctggt cccggtggtc cgggtatgca cggccccgag
     9181 caaatgccag gcggcttcgg ccacggcgtt ccgagccacg gcccgttcag tcatcgcggc
     9241 caccatcgcc atcatcatcg ccgtcgcggc cgcaatcgcg aggagcgcct cgctgccctc
     9301 gaggccgagc gcgagatctg gcgcgcgcat cccgaccgct cggcgtggct gccgtggtgg
     9361 ctgcaagcgc ggatgcgccg ccgcatcttc agctggttcg cgatcgcatt cgtgctcggc
     9421 accctcgcgg gcatttatct ctggccggat catcggtggt ggcacttcgt gctcgcggcg
     9481 ctcggcctct ccgtcatgtc aggcgcggtc tcgtggcggc tgacgcgtcc gctcatcctc
     9541 gtcgtgcaag ccgcccgcga catcggcgac ggcaagctcg acactcgcct cgatattcat
     9601 cagcgcggcg agatgaagat cctcgcgatg gcgatcaacg atatggcgtc gcgcatcgag
     9661 cagcagctca aagatcaaaa gcagctgctc gctgcggtct cgcacgagct gcgcacgccg
     9721 ctcggtcaca tgcgcgtcct gatcgagacc gcgcgcgata cgcatgatgc aaaggccctg
     9781 tcggagctcg acaaagaggt gctcacactc gacgacctcg tcgggcggct actcgcctcg
     9841 tcgcgcctcg agttcggcaa cctcgacaaa cgcacgatcg atctcggcga gctcgcgagt
     9901 gatctcgcta cgcaggcggg cgtggcgccc gagggcatcg aagccgaagg cgacgtaaag
     9961 gctgcggtcg atccgacgct cgtccgtcgc gcgatcgcaa acctcctcga caacgcgaag
    10021 actcacggcg gtggcgccgt cgccgtgcgc gtcgttcgtc gcggcccgca agtcgtcgtc
    10081 gaagtggacg atgccggccc tggtgtcggc aaagatcgcc gcgccgatgc gttccgcgcg
    10141 ttcgtgccgt cgagcggtgg cggccttggc ctcggcctcg cgctcgtgtc gcggatcgcg
    10201 gtcgcgcacg aaggcggcgc ctacattcac gatcggcccg gcggcggcgc gcgcgtcggc
    10261 tttacggtga cggcgtagca gcagctggct cgggcgcgct gcgcagccac gcgatcgcga
    10321 gacccagccc gatcaagaag ccgatccacc ggccgccctc gagcttcgcc tggaactccg
    10381 gcggcgcgga gtagccgagc acgccgctga tcagcgtccc gacgacgagg aacaggcaca
    10441 gcgcgaggcc gagcttcgtc gtacgcggca cggcgaggcc gtagaggacc agcgcgagga
    10501 acgacatcga gaacttcacg gcgctctcgg cggcttggaa ctggatcgcg ctcgctgtcg
    10561 ggaacacgcg gtgctcggcg ctctcgagca tggagatgat gtgatggtct tcgatgaagt
    10621 cgaggatcgc ggtcgcgagc atcgcgccga atgcgagcgt gacgaacggg cgaccgagcg
    10681 cgcgcagata cttcgtgaac gccgcgaaga acgcggtgta gagaatcgcg aacgcgatgt
    10741 cgaggcccat cagcgtgcgc aggcccttcg gctcggcgag cagcgacagc gcgtattcct
    10801 ccggagccat gaagtgctcg tgcttctcct gcgtggcgcc cgtgatgacg cccgtgatca
    10861 ccatgaggac gagcacgccc agcgtgcagc cggcgagcag catcgccatc cgccgcatcg
    10921 accactcttt ctcgctctcg ctcatggcga ccattgtcgc gctggccgcg gcgcctcgcc
    10981 acttatgtca ctgcgggacg agctggcgga tcacttgctc gctggtcttc agctcttcgc
    11041 ccatctccgt caggtcgtgc gtgatgtcgc cggagaggac ttccatcgcc tgcgcatcga
    11101 gcgcgcgaag ccggacgatc ttcgtcggca gcgccccgag caccgcaacg acacgcatga
    11161 gattcgcgtc gacgcgctcc ttgctcgtcc gcagctcttt gagcgcgcgg atctgttcca
    11221 tgcgtgcgtt cttcgcttga tcgaacgacg cgcgcgcctc gggatccttt gcctgcgcgg
    11281 ccttcgtcgc gagcagcttc acttcctgca cgagcgacgg caggttgacg gagcgcaggt
    11341 acgcggtcgt gtcctctgcg cgcttcacta atgatgcggc gtacagctcg agctggatca
    11401 gcgagtcgag cgtcttctgg agctgcatga ggacgtcggc cggcgtgtcg tcgatgacct
    11461 tctggaggtt ctgccgcgct tgctgcatct tcgcgacggt cgcgcgggtg tccgcatcgc
    11521 ggatgtcgtc gaggtccggc atgccgccga tgaccgactg aatgcgcttg cgcttcgctc
    11581 gcgcgcggga gcggcgaatc gcgtcgatcg cggcgctgac accccaaacc gcaccgccgg
    11641 cggcggcgat cagcggattt gtcaacacga ccgcgacggt agccgcgctg acgagggctc
    11701 caagccccac tgggttgacc agcgcgcgtg cgacagacgg cccgttgctg tccgacattg
    11761 cgagcggccg cagcatacac ggcggcgcga tcttgtcgta ccttcacgat cccttaggga
    11821 gggaccttcg atgattctcg gcaaattctt cggtgcgatc ggtgcccagt tcaacaagct
    11881 cggcaactgg ctgttcaaca aagacccgat cgcgcagatg caatacgagt acgacaaggc
    11941 cgtcgagcag ctcaaagagg gccgcgtggg cctcgagcag tatcgcgggc tagtcgagcg
    12001 cgtgagccgc caggtaaagg aaggcgacgc gacggtcacc aagctgacct cgcaggcgaa
    12061 ggcctacctg aagatgggca accgcgagat ggccgcgcag ttcgcgcccc agctgacgaa
    12121 ggccaaggcg caactcgccg agaaccagca gcagctgcag atgcacgaag aggcctacaa
    12181 caacaacctc aagaagattc agcacgccaa caagaagctg atcgagctga aggacaagat
    12241 tcacaagtac gacgcggatc tcaagatgtc cgaggccgag gccgagatcg cgaagatctc
    12301 gtcgagcttc aacatggatg tgacgaccaa cttcggtcag ctcgaagaca acatccagcg
    12361 ccgcatcgat acgaaccgag gcagggcgcg cgtcgcggcg gacatgtcgc agcagggcat
    12421 cgagaacatc aaagcggaag agcagctcga gaagtcgatg gctgacgacg cgctgaagga
    12481 tctcgaagtc gagctcggca tgcgcgcgcc cgagaccacg ccggtccaac aggcgcagaa
    12541 ggatctcggt ccggccgagg ccgaggccgc acgtaaagag ctcgagaacc tcgagaagtc
    12601 gtagccgatg gctgacgatc cgaagacgct cgcgggcagc gtcgatggcg tgacgaaggc
    12661 gacgcagctt cccgcgcatt tccctgcgtg ggcggccaag ctcgccgagc tgtatttctc
    12721 cggcacgacg tcgatgttcg tgctgcacgg caacacgttc gatgtcgtgc gtgcgggccc
    12781 cgagaatgat ccgcggtgga cgggcctcgc cgacttcctc gccgatcaat tgttcggccg
    12841 ctgggacctc gtcatccact acgacctcgc gcgtggtctg cggtgcgccg cgggcacgag
    12901 cagcaagcgc ctcgtcgaga tggtcgccac gagcaacaag tggatcggcg atctcaaggc
    12961 gctgccgcgc gatccgacga agggcctgct cgcgctcgac ctcatgatcc agaagatcgt
    13021 gatggccgac gaggacaagc gaattcgctg cgcgatcatc atcgatcacg tcggctacgt
    13081 cgccccgagc ggcgacaacc tcgacctcag cgagcagacg catctcgtca cgctgctgaa
    13141 ctgggcgtcg agcccgtaca tcaagcgcct caacatggcg ttcgtgctga tcgacccgcg
    13201 gctctcgagc atcgccgagc ggctctcgtc gaacccgcac gtcgcgcaga tcgaagtgcc
    13261 gctgccgaac gccgacgagc gcactgcgtt catcgctgcg caggtgaaat cgacgggcaa
    13321 ggagatcgcc acgttctccg actacagcgt gcccgagctc ggcaagctca ccgcgggcat
    13381 cggcctcacg gatctcgaag tgctcgtgcg gtcggcggcc gaaggcacgc ggcggctcga
    13441 tcgtcagtac ttcaaggagc tgaagaagcg gctcatcgaa cgccaggcgc aaggcatgct
    13501 cgagttcgtc gagccgaagt ggggcctcga cacggtcgtc ggtcacgagg ccgcaaagaa
    13561 gcgcctcctc gacgacgcgg agctgctcaa gcgcggcgag ctcgacacgg tgccgatggg
    13621 ctacctgttt tgcggcccgg tgggtaccgg caagagcttc ctcgcgacgt gcgtcgctgg
    13681 ctcgatcggc atcccggccg tcgtgctcaa gaacttccgc agcaagtacg tcggcgagac
    13741 cgaaggcaac ctcgagcgcg tgctcggcgt catgcgatcg atggggcctg tcgtcgtcgt
    13801 gatcgacgag gcggatgcga tgctcggcga ccgcgattcg ggcggcgatt caggtgtcgg
    13861 tggccgcgtg ttcgcgatga tcgcgtcgca gatgggcgac accgcgtatc gcggccgcat
    13921 catctggatg ctgctgaccg cgcgtcctga tcagctgccg gtcgatctca agcgccaagg
    13981 ccgcgccgag gtccacatcc cgctgttcta tccaacggat caggctgagc tcaagacgat
    14041 gctgatggtg ctcgcgaaga agtcgggcac atcgttggcg gagagcgacc tgcccgacac
    14101 ggtgcctcac atgggcaacc tatcgggcgc agacatcgag ggcatcatcg gtcgcgcgta
    14161 ccgcacctcg ctgctcggcg gaagcaagac gatcacgaag gccgcgatcg agcaggccct
    14221 cgcgggcttc atgccatcga cgcaaacact cgagcgcgaa gcccaagagc tcgccgccat
    14281 catcgagtgc accgatgtcg agttcttgcc gcagcccaag cgcgacaagc tcgataagtt
    14341 tggcggtcgc gagaagctgc aagagcggct caccgcgatc aaacagattc tagaggagag
    14401 atagccatgg cacgtacaag ctggatcgat gctgacagca accccgacct cgacgcccac
    14461 gtcagcaagc tcgagcactt caccgcgtca ctcgccgacg gcgtgatcac gtccgacgag
    14521 ctcgagaagc aggagaacaa cctggtctcg gcgatgaagt ccgtcgaggc gtcgctcaac
    14581 gacgagcagc acgccaaggt gacgaagctg ctcgcggagc tgacggccta ctcggtcatg
    14641 cgtacgctgc acgacctgac gaaggcacac gtcaaaggcc tgaagctgta agtgcgacgc
    14701 ggctgtctgc gcgtggccgc gatcgtgtcg acggtggcgg cgtgcggccg cgtggcgttc
    14761 gagacgcgta tcgacgccga ggctccgacg ccggatgctc tggtcgtcga tgccccgacg
    14821 ggcgtcacgt ttgtgaggaa catcgccgcg cagatgttca ccgcgaacgg cagcgccgcg
    14881 tcgatcccgc tgcaagcgtc ggcggctggc aatgcgatcc tcgttcacgc gacatgcacg
    14941 agtgtcacga cgccaatggt ggcgacgctc gagatgaccg ggacgtggac gttcacgcag
    15001 ctcggccccg tcgagcgcta cctgagccaa caaggagttg tgctcggagc gatcgcgccg
    15061 aatacgaatc cgacgtcgtt gctcgtcggc tttagcatcc ccgactgcgc accgttgtcg
    15121 atcgtcgcgg acgagctgac gggtaacgac cccacgggag gcacgacgac gttcgacgcg
    15181 aacgcccagg gcatcggcag cggcggttgc agcgtgccgc tcgtgaccgg gagcgatgac
    15241 gagacgctct ggggcgcctg catctcggag tttccgatca ccggcgtcgg gaccgagttc
    15301 agcaaggccg gcgatgacgg taacgaccat tggtcggagt accgcgttgg aacgggaccc
    15361 gccgggacgt cggttgcgac gtcgttcatg aatggcgtgg gcggctatct cgcggcgggc
    15421 gtcgcgatca agccgcgctg attactacgc gctggcgatc aggccgcgct tgcggagctt
    15481 cttgagctcg gtgcgcagcg ccttgtcttc gagctcttcg cgcatgcccg ggtgagcggc
    15541 gagaaacgcc tgccacgcca cggtcatgaa ggcctcgctt ggaatgccct ccgaggcggc
    15601 cgcatcggcg gcgcgcttca gcaggccagc gagatcgtcg tctcggttgt tgttggacat
    15661 gggatcaata gaagcatccc gaggtcccct cgtaactaac cgatcggtga gccgccgtgc
    15721 gagctacgcc tgcgcgactt tgccgcggcc gcgggctgcc gccatgtcga gaacctcgcg
    15781 ctcttcttcg agcttggcgc gaagcgcggg attgcacgcc aaaaatgcct ggcaggcggc
    15841 gacgaggaac tgctcgctct ctacggaatg tgctgtggcc agttgggcag cgcagcgcag
    15901 caaacgttgt agctcgaggt tgtcgtcgtc gttggggatg ggcattcggg aacaacttga
    15961 gcacgggtgc ggagaatggc gagcggtctc acctcggtga gaatctcgca cgcgtcgcgt
    16021 cgcgagaaat tccgattcgc tagaacgcgg tgccggcggt ccaatctgtt tcgaagctcg
    16081 tcgagagcgg cgcgatcgag gcagcgtccg tgacgatgag gccgacctcg cgattcttgt
    16141 cgagcgatgt ttgcgacagg ttctccgagc cgagatatgc gcgcgcaccg tcggcgacga
    16201 tcaccttcgt gtgcagatgc gggatccact tcacggtgac gccgaggcct ttgaggtagt
    16261 cagcagcggc tgcattggcg ctcacgaagc cggcatccgc gatcagcgcg cggacgtcga
    16321 cgcccgcttg cacgcgcgcc ttcacggcct cgcgaacttt gtagtcggcg aactgcatcg
    16381 actcgatcgt aagcgtctgc gtcgcgctgt tgatgagatc gaggatgcgc gtacgcgagt
    16441 tgatcggcga gatgaccatg cgcgtgcacg gcatctgcac gggctggccc gtccagtctg
    16501 cttcgaacag cgccacgaga tcggcgaggt ccgcgggatc gcgatccgtc gcgacgtgat
    16561 tgcgctcgag ctcgatcgag tagctcttcg agtagttgcc cgtcgagatg acggccgtcg
    16621 tgccgtcgac gacgaggacc ttcgagtgct gatacgtgaa ctcggcgctg ctccacttca
    16681 cttcagcgcc cgcagcggcg agctgcgtga agtacttctg gttcgtgtcc atcttgtact
    16741 ggtcgaggat cacgcggacc ttgacgccgg cgtgcgcttt cgcgacgagc gtgtcgagga
    16801 tgccgccgta gcccatcagg tagatctcga cgtcgatcga gtgctgcgcg ctcgtcagcg
    16861 catcgagata cggctgctcg ccggcatccg gcgtcaccgc gacttcgacg ggcatcgcgc
    16921 gcggatccgt cgcgttgcag aagtcttcga tcggctcggc gctgtcttgc ttgccgccgg
    16981 cgccatccgg tggcgtcgag gcagggtctg aggtgtcggc gcacgcgccg gcgaggagga
    17041 cgaagagagg cagggcagag gacagccgca tgccctacaa gtcgcggctc gcccgacttt
    17101 gatcccgaaa atcttcgcga gcgatcccgg tgccttacgc cggcgactac ttgaccttcg
    17161 tgaccgcgtt gcccttgccg accgaggcga ccttgggctt cttgcccttg ccgagcacct
    17221 tcttgtacgt gaccttgttc ttcgtgccgt tgatgtttgc agcgtgtagc gccgtcgtct
    17281 tgatcacgtt actcgcgccg ttgacgttga gcgtctcgac actgtcgagc gtgatctgca
    17341 cggtgctgcc gttgacgttg atctctttgc actcaccggt gaacgtgaac gtggcttcgc
    17401 catagttgac gttgacggtc gcgtcgttcg cgcagtccca cgtggttccg tcggtcggct
    17461 cggacagatc tttgtcggcc agtgcggtcg atggagcgag caaagcaaca gcaagaagca
    17521 gcttgcgcat ggaaatcccc cggtgccctt agacggccac cttcggccgt cattcaaccg
    17581 agcttacacc gtcaggcgcg cgcgaacgcg acctgcagga cccgcatcga cctcgacgac
    17641 gtcgccgggt cgcatcaatg cgttcgacgt cgcgtacgtc gagatgaggt actcggcttt
    17701 gcggaaccga tcgctgacga ggcgcgcgag cttgcggtcg agccacgata gcttcatgcc
    17761 gacgcctgcc ggtgtacccg tcaggatcac gtcgccgcgg ccgagcggtc ggccgagctg
    17821 ctggcgtgca gcgcggacga ttgcaggcag atcgtagagg aggtctcgcg tcgtgccgcg
    17881 ttggcgctct tcgccgttga cgcgcgtgac gagctcgagc tccggcagct tcgcgaaccc
    17941 gcctttgggc gcccacacgc gcggcgcaac gggcagaaag cgcgggaacg atttcgcgca
    18001 cgcccagtag gcgagcggcg tctgcgtgcc ttcgccgaac acttggcaga tgcgcgcggt
    18061 gagatcgttc gccgcggcga ggccgagcgg ttgtggcgtg cctgcggtga gtgcgtcttc
    18121 gtcgatgtcg ccgagcgcga cgagcgcaag ctcgccttcg tagtccatca ccgcgggcat
    18181 gcgtgcgatg cgatcgcgca gctttgcgtc gaggcccggt tcgacgtcgg cgagcgcggc
    18241 gagcagttgg tcgtacgtcg ggactgcgac gtggatgttt tgctcgaggc tgttcgcgat
    18301 gaacgaccgc agatgcttcg cgaacgtcgc gggcggtgtg ccggcgtcgt gtgtctggcc
    18361 ggtctctttc aagtgatcgg cgtacgtgag cccgagcgcg acgatcgatt ccacgggcgg
    18421 ccaatcgacg gagtccgacg cgatgttcgt gcgatcgatc cgcggcgccg ccgacgcgtc
    18481 gtcccactcg atgtacgaga cgcccggcag cttcgcccgc tgatggatct cgacggcgac
    18541 gccgcccgcg acgcgacgtt cctcgacgag cttccatggc aggcccggcg cggtgcgctg
    18601 aaacgattcg tagtacgcgc tgccgatgtc gctctgcgcg accatgagat cgcggccgtc
    18661 ggtatcgcga taggcatagc cgaccagctc gtcggcatcg gcccatgcat gcgcgtgcac
    18721 gaggcggcgc gtccccgcaa tcgtcgatgt gacgaggccg tgcggatgtg cggccgtggc
    18781 cggagtgcct gtgatgccat cgcgtccgtc gagcgagagc gacgagatgc cgagcgagaa
    18841 cgtgtcgcgc agagagacgg cagtcacctc gagcgagcca ttaccgagcc acggcgtcca
    18901 cacccagtgc agcgtcggca cgcgcttctt gccccacaaa tgcatcgcga acgctttgcc
    18961 gcgcagcgcg attggcttgc cgtcgacttc gatcgtcgct tcgcagtccg cgtcgtgcgc
    19021 gagctctcgc gcgtgcgtcg gcgcggggag ccacgtcgga acctcgtcac gtaccggctt
    19081 gcctccgctc cacgtcgcct tccacgtgag gccgtcgatg ccaccgacga cacccgtgcg
    19141 cgcgacgtga ctgtccgcga tccggatcag ctgttcgcct tcacccgtga tcgcctgatc
    19201 gagcggaagg atgcgctttc ccgcacgcgt ttttggcatc gcatcggcgt cgaaccaggc
    19261 gccccaaatc gtcgctcggc cgtccccttc tttggggatg aacagcgatt cccggagcca
    19321 caacgcgcgg cgccggttct cgtcgaggag gacggcaaac cagatctcga accaggggcg
    19381 ttggccacgc cagagctggt ggtcgagatc cttagccatt gcctggacat ataccgccac
    19441 cgcgcgctgc gctatagggt gtaagccgtg ggagtggttg ggggctcttt atctgcagcg
    19501 tgcgaatcat gtcccttacc cacggagcac ggctagacgt ggcatcccgt accgaagacc
    19561 gcggccgcct gctgtccgag ctggccggtg ctcccgcgcg caaggggtat ggcatcgcct
    19621 acgacttgct cggcaaccga gctgaagcag aagaagccgt gcaagaggcg ctcgcacgcg
    19681 cgtgcgaatc gatcgccgac ttgcgcgatc ctgttgcagc acccgcgtgg tttttgcgca
    19741 tcgtgacgac gatgtgcctc cgcacgttgc ggcgcagaaa gctcaagcgc gcgctgttcg
    19801 gctggatgcc cggcaaggac gactccgagc cgaagccgga cgcgagcacc gatgcgatcg
    19861 ccgaacgcat gcacggccct accggccaag cgcctgtcga agcgctcacc gatcgcgaag
    19921 aatttggcgc gctgatgagc cgtctcgatc agctcagcgc ccaacagcgc accgcgctcg
    19981 tgctccgcta cggacacgac ctcggcgtgc cggaaatcgc agagctcctc ggcgtcgagc
    20041 tcgcgactgc aaagacgcat ctcgtgcgcg gcctcgcgag gctgcgcgat ctgatggagg
    20101 agcaccgatg accacttgtg atgtagttgc cgaacgtgtc gcgctcggtg agcctctcgg
    20161 agatgcggcc gatcatgcag agcgttgtcc gaagtgccgt cgcctcgtcg cattgccgac
    20221 ggagctaggc gcgacgcacc gcgaagcaga tccgggcctc ggcttcgcag cacgcatgac
    20281 agcgggtgca caacatcgcg tggtcgtgcg gcgccgccgc cgaatcgcgg caggcatggc
    20341 aggcgtcgtc gctgcagcgg cgatggctgc cttcttcgtc acgcgcgagc ccgacgcgcc
    20401 gaagctcgca acgacgccgc ccgatcagac gcagcagccc gcaacgaacc cgcacgaagc
    20461 caacaagaac gatccgtggg acgcgagcgc tagcagcgac gtcgacgaag acgtgcgcgc
    20521 gctcattcag ctcgcggaca ccgacgcggc gtctcgcacg accgcgcact ggaagaagat
    20581 cgaacagcca ctcgatc
//
LOCUS       DATLBZ010000147        10313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_139784, whole genome shotgun sequence.
ACCESSION   DATLBZ010000147 DATLBZ010000000
VERSION     DATLBZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10313
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139784"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="VMZ53_07845"
     CDS             complement(<1..1550)
                     /locus_tag="VMZ53_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUS28404.1"
                     /translation="MVRNTVVVAFVLVVASELAALVSPAFPRDVAGTAIMASAFVIIA
                     AACVFGGSRELVTALTVLMFGVLGWYVARAMGNSGGLHSSHRDAIYSVLAGAYALLLF
                     SALEFTAVTILFTGTMAVTALARGDVTIAELGLPGFYLFSFYSLAMVGIMARTRLKRS
                     EREARDELERLNANLRSEVAVQVSRIQRAETLSRYLPPELTEQVLASDAGDALAHGHR
                     DVAVLCASPVGFLESLARLTTDQVAALVNSFVTAMSRVAFDHGGVIERFVGPRLTVLF
                     GSLAEQELSESVKRAVAMGREMQRSCNVLLLQWEHEGLPIHLRMAIGVTAGASVVGTF
                     GSERRVEYSALGEPMVRAHRLTATAIPGEIRLDAAAAKWMNDVEIGPGEPVEFKPGRP
                     EPTYVIDPHRVTPLPVESGTENTALNAALLATGMPPPADSLALGATLTSLTPKGLTPS
                     TRVAPPTLQPGQLFDGRYRIEARIGRGGTATVYRAQHVALHEPRAIKLLHPESLETPG
                     AVEQLRREV"
     gene            complement(1636..2256)
                     /gene="pssA"
                     /locus_tag="VMZ53_07850"
     CDS             complement(1636..2256)
                     /gene="pssA"
                     /locus_tag="VMZ53_07850"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HUS28405.1"
                     /translation="MPSSRPRKAMIFELRLADWFTLANAACGMGALFSSITYLETHEV
                     HHIYRTCGLIVAALVFDALDGRIARWRKSHSLLGRELDSLADVISFGVAPAMLAFVCG
                     MHGLLDKIVLGYFVACGVSRLARFNVTAAELSEGSDKVSYFEGTPIPTSVLLVALVTV
                     AAATGHVREHLWLGEVSLGSLVLHPLVILFAISGTLMISRVRIPKL"
     gene            2286..2981
                     /locus_tag="VMZ53_07855"
     CDS             2286..2981
                     /locus_tag="VMZ53_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28406.1"
                     /translation="MRRVLVVLIGVAACNGVHPDYCDGNVLHFCSEDDHGSFCSTTDC
                     GAAAQTCVEAGDGAAALCVSDPTPNPACASAGGAPRACDGANLLECTYEYATRAVDCG
                     APELCDARSRYCLKRPGEDATCATLTTDGLLGASYCAGQYSRSCTDMFVTGEWDCGST
                     ELCHAGPNGFTACIQSTTPDARCPTPAAGGTGFSSFCDGWFAVSCFDGYVHRYEDCMN
                     PDEYGSGCPLCGH"
     gene            3062..4282
                     /locus_tag="VMZ53_07860"
     CDS             3062..4282
                     /locus_tag="VMZ53_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28407.1"
                     /translation="MVSVGSENEALAGLLATISDRRIRRKVQKLLQLGEEAVRALGEI
                     DAALYLREDGEEQLQIVADAVLSHFRRLLEYVVMTAPAPAATGADEGGGDLELEARGS
                     ARELGATPKRSMIADRARSDDEKWAALVREMGTFQYGLGFELKEFERRFARALENDEK
                     EQAVRDLNDATTGLTDGVFAVMTTVYECFLGYAEPERMVAGHRDTLGKALAVRRAIAE
                     LRRGVRGLNRRVQDRTTPHAVAEAHYRRIVDAMSRFIDSDVFVYLRSDTRRDFGQFQE
                     TLGNGDVMQNRLDCEGFDKYLDSLSFVSQRGVLIKHDTDLKKKIAGDLERAVKFAALL
                     PDVACDVIEQAFVKAERLSGLSDEIDGLVLQWSSMTAEDRVDVDLAAGLGRQLLDLVR
                     TAPRAPSHDDGDSF"
     gene            complement(4284..5318)
                     /locus_tag="VMZ53_07865"
     CDS             complement(4284..5318)
                     /locus_tag="VMZ53_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS28408.1"
                     /translation="MRVLVIDEGFMSGAYAALGLHAHGCAVDVLAAVGGRARCLATGG
                     EWQFVPRVGTSALAAVLDASTRNGGYDVVYPATEPLQQLLHATPPAFRDKRVMSALMK
                     SAGIAVPAEHPAETDDDVRAAGHALGLPLVIKGSVGRGGNATFIVRSIAAAQRVARRM
                     RARGGEVFAQEHIAGVTYLAGGVFDDGRAIRLYCGAKRVQFPARTGPAAELESSSDPA
                     LVETALRVFAAARVTGLASADFLRAADGRYYFVELNPRPWGSITAARDAGVDLFGPLV
                     ALWRGQTLPPELQFRPTVVSPVFPFALLAVPCWRSGAALRALGSRPVREPKLMAHIAH
                     RLMRASRTML"
     gene            5400..6509
                     /gene="tgt"
                     /locus_tag="VMZ53_07870"
     CDS             5400..6509
                     /gene="tgt"
                     /locus_tag="VMZ53_07870"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HUS28409.1"
                     /translation="MPFEIRAQAAGTRARTGVLTTRRGTIETPVFMPVGTRGTVRTQS
                     LTKVEQLGAPILLANTYHLMQQPGIEVFERCGGLHRWMGWSGSILTDSGGFQVFSLAH
                     ARTIDDAGAVFQDVVDGPRLVLTPERSIAMQQAIGADVMMVLDHCVAADTTLEETRDA
                     MERTHRWARASLAARTQAQLFAIVQGGRFPELRRQSAAELTSIDGFDGFAIGGLAVGE
                     ERAEREDITELVAELLPPDRPRYLMGVGMPVDIVEAVHRGVDMFDCILPTSLAQHGRV
                     FSQVGRVDLRKTLHRFDEAALDPTCTCEACTRHSRSYLHHLVKAREPLGWQLLAEHNL
                     RFYVRLMQDIRTAIREGRFAELRASATRWREGASD"
     gene            complement(6569..7114)
                     /locus_tag="VMZ53_07875"
     CDS             complement(6569..7114)
                     /locus_tag="VMZ53_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28410.1"
                     /translation="MSRHVRCALALSLGLGIAGCSHKKVEITQSFTMTPGPIERLTVF
                     ISMPEGRDYGVYPGFKRVMTSELAACKVASQVFVGLPPEASDAAAPSTRDPQLVVKAR
                     YGVLESTKVVNQRGALLDDKGVTNVKADFWMELREPPQDRVSWRAFAKMESDPGSGGG
                     EAFAESIVARLKSDGVLHGCR"
     gene            complement(7169..8383)
                     /locus_tag="VMZ53_07880"
     CDS             complement(7169..8383)
                     /locus_tag="VMZ53_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS28411.1"
                     /translation="MRNVIFAAPFPLETTMRFARAAARLPGVRLLGVAQTPPTGEDAR
                     LFADVVQVEDGLDTGQLIQAGRIFQGRHGNIHRLIGILEPLQVQLAHARRALGIAGTS
                     PETADLFRDKARMKEELRRHGLPCARHRLIKSWADAESFVREVGFPLVLKPPAGMGCK
                     ATWRINSTDELGAALRALHASPQNPALAEEFLRGREHSFETITINGKVRFESCSRYYP
                     TPLEVMETPWIQWVVVLPRVIDGPDYADARALGVRAVEALGLDTGFTHMEWFRRDDGS
                     LAIGEIAARPPGAHIVLANSYAHDADMYRAWARATIDDAFDGPFERKYAVGIAYLRGV
                     GHGRVVRVAGVEEANHRVGRLVVDSRLPSTGTPRSDSYEGDGYVIVRDPDVEVVKAAM
                     TTVIETIQIEYG"
     gene            8410..9627
                     /locus_tag="VMZ53_07885"
     CDS             8410..9627
                     /locus_tag="VMZ53_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS28412.1"
                     /translation="MKVVFLAPSYPPEMQQYTRGLAEVGAQVYGVGDQPVGSLPRSLK
                     QYLTDYLQVPKLLAEDDVVNRVHAWMRGREVDRVLANWEIMVLTAARLRERFGVPGMT
                     VNQVRGFRDKQLMKERVAAAGLRVPKSVRVATATEAREGAARIGYPLVLKPISGAGSA
                     DTYAVGSAQELETTLARMQHVGEASLEEFIDGEEFTYDTVCIGGKPAFANVAQYLPKP
                     LIARSTEWISPVIVTVRDLAQPALAGGIELGDRVLGALGMGDGFTHMEWFRKPSGEVV
                     FGEIGCRPGGAHLVDQMNYTCDIDLFREWARAVCWNTFEAPTRRKYNAAIIFKRAKGQ
                     GRISRITGLDEFMRQYGEHVVESKLLPVGAQRRNWKHTLVSDGYILLRHPDWATACKL
                     ADIVATQVEMYAE"
     gene            complement(9630..>10313)
                     /locus_tag="VMZ53_07890"
     CDS             complement(9630..>10313)
                     /locus_tag="VMZ53_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUS28413.1"
                     /translation="TVNRQAARRPVSEWERARLDEANTSKGNYARVENYMETDLFTVH
                     AEDAIEMVVNLMIWERIRYVCVEDSDHNLVGLVSHRAVLRFVQSGRSTLRTPISEIMR
                     REVTTVSPETATLDALRMMRRLQVGCLPVVDRERLIGIVTEGDFLEIASKLLEHELAA
                     DDVVAEEPSRQLELPQTGPTARITPEREAQIAAALPERDFDREIDTDHQTAASIDDIP
                     APRDEADEA"
BASE COUNT         1790 a         3368 c         3485 g         1670 t
ORIGIN      
        1 acttcgcggc gcagctgctc gaccgcgccg ggagtttcga gcgattcggg gtggagcagc
       61 ttgatcgctc gcggctcgtg gagcgcgacg tgctgtgcgc ggtagacggt ggccgttccg
      121 ccgcgaccga tcctcgcctc gatgcgatag cggccgtcga agagctgtcc cggctgaagc
      181 gtcggcggcg ccacgcgcgt cgatggcgtg aggccttttg gcgtgagcga tgtcagcgtt
      241 gcgccgagcg cgagggagtc agccggtggc ggcatgcccg tcgccagcag cgcggcattc
      301 agagcggtgt tctcggtgcc gctttcgacg ggtagtggag tgacgcggtg cggatcgatg
      361 acgtacgtgg gctcgggacg cccgggtttg aattcaacgg gctcgccggg cccgatctcg
      421 acgtcgttca tccatttcgc agcagccgcg tcgaggcgaa tctcgcccgg gattgccgtc
      481 gccgttagcc ggtgcgcgcg gaccatcggc tcgccgagcg cggagtattc gacgcggcgt
      541 tccgagccga acgtaccgac cacggacgcg cctgcagtga cgccgatcgc cattcgcaga
      601 tggatgggca gcccttcgtg ctcccactgc aggagcaaca cgttgcagga gcgctgcatc
      661 tcgcgtccca tcgcgaccgc acgcttgacg gactcgctga gctcctgctc ggccaggctg
      721 ccgaacaaca ctgtcagccg cggcccgacg aagcgctcga tcactccgcc atggtcgaac
      781 gcgacgcgcg acatcgcggt cacgaacgag ttgacgagcg cggcgacttg gtcggtcgtc
      841 agtcgagcaa gactctcgag gaaaccgacg ggcgaggcac acaggacagc gacgtcgcgg
      901 tggccgtgag cgagcgcgtc gccggcgtca ctcgcgagta cctgctcggt gagctcgggc
      961 gggagatagc ggctcagcgt ctcggcgcgt tgaatgcgcg agacttgcac ggcgacctcg
     1021 ctgcggaggt tcgcattcaa tcgctcgagc tcgtcgcgcg cctcgcgctc ggaccgcttc
     1081 agccgcgtgc gcgccatgat cccgaccatc gcgagcgagt aaaacgagaa caggtagaag
     1141 ccaggcagac cgagctctgc gatcgtgacg tcgccgcgag caagcgcggt caccgccatc
     1201 gtccctgtga acaggatcgt gaccgctgtg aactcgagcg cggagaacag cagcaacgcg
     1261 tatgcgccgg cgagcaccga atagatcgcg tcgcgatgac tcgagtgcag tccaccgctg
     1321 ttgcccatcg cgcgggctac ataccagccg agcacaccga acatcaggac ggtcagtgcc
     1381 gtgaccagct cgcgcgagcc accgaagacg caggcggcgg cgatgatgac aaacgcgctc
     1441 gccatgatcg ccgtgcccgc cacgtcgcgc ggaaacgcgg gcgagaccaa cgcggcaagc
     1501 tccgacgcga cgaccagcac gaatgcaacg acgacggtgt tgcgcaccat cgccacgaag
     1561 cgctcgttat ccaagtcgcg cgcaacacca cccgcaatac gggagattag attatcggcg
     1621 cgccgagaca tcgcgtcaga gcttcgggat tcgcacgcga cttatcatca gagttccgga
     1681 gatggcaaac aggatcacga gcgggtgcaa cacgaggctt ccaagggaca cctctccgag
     1741 ccacaggtgc tcacgcacgt gccccgtcgc ggcggcgacg gtgacaagcg cgactagcaa
     1801 gaccgacgtc ggaatgggcg tcccttcgaa gtagctgacc ttgtcggagc cctccgagag
     1861 ctcggccgca gtcacgttga agcgcgcgag acgcgacaca ccgcacgcta cgaagtagcc
     1921 gagcacgatc ttgtcgagca gcccgtgcat gccgcagaca aaagcgagca tcgcaggcgc
     1981 cacgccgaac gagatcacgt cggccaggga gtcgagctcg cgaccgagca acgagtggct
     2041 cttgcgccag cgtgcaatgc ggccatcgag ggcgtcgaag accaacgccg cgacgatcaa
     2101 gccgcacgtg cgatagatgt gatggacctc gtgggtctct agatacgtga tcgaggagaa
     2161 cagagcgccc attccgcacg cggcgtttgc gagcgtgaac cagtccgcga gacgcagctc
     2221 gaagatcatg gccttacgtg gccgggacga cggcatggcg catgagaaca tgatttcatt
     2281 ggggcgtgcg gcgtgtgctg gtagtcctca tcggtgttgc ggcctgtaac ggggtccacc
     2341 ccgactactg cgacggcaac gtcttgcatt tctgctccga agacgaccac ggctcgttct
     2401 gttcaacgac cgactgcggc gcggcggcgc agacctgcgt cgaggccggc gacggcgccg
     2461 ccgcactgtg cgtcagcgat ccaacgccga atccggcgtg tgcttccgcg ggcggtgccc
     2521 ctcgcgcgtg cgacggcgcg aacctcctcg agtgcaccta cgagtatgcg acgcgcgctg
     2581 tcgactgcgg cgcccccgag ctctgcgatg cgcgctcgcg atattgtctg aagcgtccgg
     2641 gcgaagacgc gacctgcgcg acgctgacga cggatggcct gctcggcgcg tcctactgcg
     2701 cgggtcaata cagccgtagc tgtaccgaca tgttcgtcac cggcgagtgg gattgcggca
     2761 gcacggagct gtgccacgcc ggcccgaacg gcttcactgc atgcatccag tcgacgacac
     2821 cggatgcgcg ctgccccacg cctgcggccg gcggcaccgg cttctcctcg ttttgcgacg
     2881 gctggtttgc ggtgagctgc ttcgacggct acgtccaccg ttacgaggac tgtatgaacc
     2941 ccgacgagta cgggtctggc tgcccgctct gcggccacta gatccgcaaa tctttgagcg
     3001 acgttttcgc tacccctatg ttcaggtggc ggcgagattc gtgcgcacct atgctttcgg
     3061 ggtggtgtcg gtcgggtccg agaatgaagc acttgccggg ctgctagcca caatctcgga
     3121 tcgtcggatc cgccgaaagg tacagaagct gctgcagctc ggcgaggaag cggttcgcgc
     3181 gcttggcgag atcgacgctg cgctctacct gcgtgaagac ggcgaggagc agctccagat
     3241 cgtcgccgat gcagtgctga gtcacttccg gcgactgctc gagtacgtcg tcatgaccgc
     3301 gcccgcgccg gcagcaaccg gcgccgacga gggcggtgga gacctagagc tcgaggcccg
     3361 cggttccgct cgtgagcttg gcgcgacgcc taagcgcagc atgatcgcgg atcgcgcacg
     3421 ttcggacgac gagaagtggg cggcgcttgt ccgtgagatg gggacatttc agtacgggct
     3481 gggattcgag ctcaaagaat tcgaacggcg cttcgcacgc gctctcgaga acgacgagaa
     3541 ggagcaggcc gttcgcgatc tcaacgatgc gacgaccgga ctcacggatg gcgtcttcgc
     3601 ggtgatgacg actgtctacg agtgcttcct cggatatgcc gagcccgagc gcatggtggc
     3661 cggccatcgc gacacgctcg gcaaagcatt ggctgtgagg cgcgcgattg cggagctgcg
     3721 acgcggagtg cgtgggctga accggcgcgt tcaggatcgc acgacgccgc atgcggtggc
     3781 ggaagcgcac tatcggcgca tcgtcgacgc gatgagcagg ttcatcgaca gcgacgtgtt
     3841 cgtgtacttg cgctccgaca ctcggcggga cttcggccag ttccaggaga ctctcggcaa
     3901 tggcgacgtg atgcagaatc gcctcgactg cgaaggcttc gacaagtacc tcgactcgct
     3961 atcgttcgtc tctcagcgcg gcgtgctcat caagcacgac acggatctga agaagaaaat
     4021 cgcgggcgat ctcgagcgag cggtcaagtt cgccgcgctt ctgcccgatg tcgcgtgcga
     4081 cgtgatcgag caggccttcg tgaaagcgga acgcctctcg gggctgagcg acgagatcga
     4141 cgggctcgtg ctgcagtggt cgtcgatgac ggcggaggac cgtgtggacg tcgacctggc
     4201 ggcagggctt ggccgccagc tgctcgatct cgtccgcacc gcgcctcgcg cgccctcgca
     4261 cgatgacggc gacagcttct gagtcagagc attgtgcgcg atgcgcgcat gagccggtgt
     4321 gcgatgtgag ccatgagctt gggctcgcga accggccgcg aacccaatgc gcgcaacgcg
     4381 gcgccggaac gccagcacgg gacagccaac aacgcgaacg ggaagaccgg cgagacgacc
     4441 gtcgggcgaa actgcagctc cggcggcaac gtctgaccgc gccacagtgc gacgagcggc
     4501 ccgaacagat cgacgcccgc atcgcgcgcg gctgtgatcg atccccatgg gcgagggttg
     4561 agctcgacga agtaatagcg gccatcggcg gcgcgcagaa agtccgcgct cgccagtccg
     4621 gtaacgcgtg cggccgcaaa cacgcgcaat gccgtctcga cgagcgcggg atcgctcgac
     4681 gactcgagct ccgctgcagg ccccgtgcgc gcgggaaact gcacgcgctt cgctccgcag
     4741 tagaggcgaa tcgcgcggcc atcgtcgaac acgccacccg cgagatacgt gacgcccgcg
     4801 atgtgctcct gcgcgaagac ctcgccgccg cgtgcacgca tgcgacgcgc tacacgttgc
     4861 gccgccgcga tcgatcggac gatgaacgtc gcgttgccac cgcgtccgac actgcccttg
     4921 atcacgagcg gtagtccgag cgcatggccg gcggcccgca cgtcgtcgtc ggtttcagct
     4981 gggtgctccg ccggcacggc gataccggct gacttcatca atgcgctcat gacacgcttg
     5041 tcgcgaaacg ccggtggtgt cgcgtggagc agctgctgca gcggctccgt cgcgggatac
     5101 acgacgtcgt agccaccgtt gcgcgtggag gcatccaaga ctgcagcgag agctgaggtg
     5161 ccgacgcgcg ggacgaactg ccactcaccg cccgtcgcga ggcatcgcgc acgcccgccg
     5221 acagccgcga ggacgtccac cgcgcatccg tgagcgtgaa ggccaagagc ggcgtacgcg
     5281 ccactcatga atccttcgtc gatcaccaga actctcatgc gtacgcagcg tccgacgaac
     5341 gtgcgatgct tccaaggcca ttcacgcgaa gttcacgctc gtgcgacgaa gctaatacga
     5401 tgccgttcga aattcgcgcg caggcagctg gaacgcgggc acgtacgggc gtgctgacga
     5461 cgcgacgcgg aacgatcgag acgcccgtct tcatgccggt cggcacgcgc ggcacggtgc
     5521 gcacgcaatc gctgacgaaa gtcgaacagc tcggcgcgcc gatcctgctc gcgaacacgt
     5581 atcacctgat gcagcagccg ggcatcgaag tcttcgagcg ctgcggtggt ttgcaccgct
     5641 ggatgggatg gagcggctcg atcctgacag actcgggtgg ctttcaggtc ttctcgctcg
     5701 cgcacgcgcg cacgatcgat gacgccggcg ccgtgttcca ggatgtcgtc gacggcccgc
     5761 gcctcgtgct gacgcccgag cgatcgatcg cgatgcagca ggccatcggc gccgacgtga
     5821 tgatggtgct cgaccactgc gtcgctgccg acaccactct cgaggagacg cgcgacgcga
     5881 tggagcgcac gcatcgctgg gcacgcgctt cgctcgccgc acgcacgcaa gcgcagctgt
     5941 tcgcgatcgt gcagggtgga cgcttccccg agctgcggcg gcaatcggcg gccgagctga
     6001 cgtcgatcga cggcttcgac ggctttgcga tcggagggct tgcggtcggc gaggagcgcg
     6061 cggaacgcga ggacatcacc gagctcgtcg ccgagctatt gccgccggat cggccgcgct
     6121 acttgatggg cgtcggcatg cccgtcgata tcgtcgaggc cgtgcaccgc ggggtcgaca
     6181 tgttcgactg cattctgccg acgtccctcg cgcaacacgg ccgcgtgttc agccaggttg
     6241 ggcgcgtcga cctgcgcaag acgctgcatc gcttcgacga ggccgcgctc gacccgacgt
     6301 gtacctgcga agcgtgcacg cggcacagcc ggtcgtacct gcatcacctc gtcaaagcgc
     6361 gcgagccact cggctggcag ctcctcgccg agcacaacct tcgcttctac gtgcgcctca
     6421 tgcaggacat ccgcaccgcg attcgcgaag ggcggtttgc cgagctgcga gcgtccgcaa
     6481 cgcgctggcg cgaaggcgcc tcggattgat tcacgccgtc gccttcgtag ctgtcggagc
     6541 gcggcgtccc acggcgacag cgacagcttt agcggcagcc gtgtagcacg ccgtccgact
     6601 tcaatcgggc gacgatcgat tcggcgaacg cttccccgcc accgctgcca gggtcgctct
     6661 ccattttcgc gaacgcgcgc cagctgacgc ggtcctgcgg cggctcgcgc agctccatcc
     6721 agaagtccgc tttgacgttc gtgacgccct tgtcatcgag gagggcgccg cgttgattga
     6781 cgaccttcgt gctctcgaga acgccgtaac gcgcctttac gaccagctgg ggatcacgcg
     6841 tcgagggtgc ggcggcgtcg gaggcctcgg gcggcaagcc gacgaagact tgcgacgcga
     6901 ccttgcacgc tgcgagctcg ctcgtcatga cgcgcttgaa cccggggtac acgccgtagt
     6961 ctcgaccctc gggcatgctg atgaacacgg taaggcgctc gatcggcccc ggcgtcatcg
     7021 tgaagctctg cgtgatctcg accttcttgt gcgagcagcc cgcgatgccc aaaccgagcg
     7081 agagcgcgag agcacaacgt acatgccggg acatcagcgg cctatacgac ggcggccccg
     7141 gcagtgtcga cgtaaatccg ctaacgggct agccgtattc gatctggatc gtctcgatca
     7201 ccgtcgtcat cgccgccttg acgacctcga cgtccggatc gcggacgatg acgtagccat
     7261 caccttcgta gctgtcggag cgtggcgtgc ccgtcgacgg caatcgcgaa tcgacgacaa
     7321 gacgccccac gcggtggttt gcttcttcga cacccgcgac gcgcaccaca cgcccgtgac
     7381 cgaccccgcg caggtaggcg atgccgaccg cgtacttgcg ctcgaatggg ccgtcgaagg
     7441 cgtcgtcgat cgtggcacgc gcccacgctc tgtacatgtc cgcgtcgtgg gcgtagctgt
     7501 tcgcgaggac gatgtgtgcg ccgggcgggc gcgcagcgat ctcaccgatc gcgagcgagc
     7561 cgtcatcgcg gcggaaccac tccatgtgcg tgaatcccgt gtcgaggccg agcgcttcga
     7621 cagcgcgaac accgagggcg cgcgcatcgg cgtagtcggg accatcgatc acgcgcggca
     7681 gcaccacgac ccactggatc cacggcgtct ccatgacctc gagcggcgtc gggtagtaac
     7741 gcgagcacga ctcgaagcgc accttgccgt tgatcgtgat cgtctcgaag ctgtgctcgc
     7801 ggccgcgcag aaactcctcg gcgagcgcgg gattctgggg actggcgtgc agagcgcgca
     7861 acgcggcgcc gagctcgtcg gtcgagttga ttcgccacgt cgccttgcag cccatgccgg
     7921 ccggtggctt gagcacgagc gggaagccga cttcgcgcac gaacgactcg gcatcggccc
     7981 acgacttgat caggcgatgc cgcgcgcaag gcaggccatg gcgccgcagc tcctccttca
     8041 tccgcgcctt gtcgcggaag aggtcggcgg tctcggggct ggtgcctgcg atcccgagcg
     8101 cgcgccgagc gtgggcgagc tggacttgca ggggctcgag gatgccgatc aggcgatgga
     8161 tgttgccgtg ccggccttgg aagattcgcc cggcctggat gagctggccg gtatcgagcc
     8221 cgtcttcgac ctggaccacg tcggcgaaca gccttgcgtc ctcgccggtg ggcggcgttt
     8281 gcgcaactcc aagcaggcga acgcccggaa gtcgagcagc agcgcgcgcg aaacgcatcg
     8341 tcgtctcgag tgggaacggc gctgcgaaga tgacgttccg catgccgcgc gttgtagcat
     8401 cgcttggcga tgaaggttgt gttcctggcg ccgagctacc cgccggagat gcagcaatac
     8461 acgcgtggcc tcgccgaggt tggggcgcaa gtctacggag tgggcgatca gcccgttggc
     8521 tccctgcccc gctcgctcaa gcaatacctg acggattatc tccaggtacc gaagctcctc
     8581 gccgaggatg acgtcgtcaa ccgcgtccac gcgtggatgc gcgggcgtga agtcgaccgc
     8641 gtgctcgcca actgggagat catggtgctc accgcggcgc ggctgcgcga gcgattcggc
     8701 gtgcccggta tgacggtcaa ccaggtccgc gggttccggg acaagcagct gatgaaggag
     8761 cgcgtcgccg ccgctgggct gcgcgtgccc aagtccgttc gagtggcgac cgcgacggaa
     8821 gcgcgcgagg gtgctgcgcg gatcggctat ccgctcgtgc tcaagcccat ctcgggtgcc
     8881 ggcagcgcgg atacgtacgc agtgggcagc gcacaagagc tcgagacgac gctggcccgc
     8941 atgcagcacg tcggcgaagc cagcctcgag gagttcatcg acggcgagga attcacgtac
     9001 gacaccgtgt gcatcggcgg caaaccggcg ttcgcgaacg tcgcgcagta cctgcccaag
     9061 ccgctgatcg cgcgctcgac cgagtggatc agccccgtga tcgtgacggt gcgcgacctc
     9121 gcacagcccg cgctggccgg cggcatcgag ctcggcgacc gagtgctcgg tgcgctcggt
     9181 atgggcgacg ggttcacgca catggagtgg tttcgcaaac cgagcggcga ggtcgtgttc
     9241 ggcgagatcg ggtgtcgccc cggcggcgct cacctcgtcg atcagatgaa ctacacgtgc
     9301 gacatcgatc tgtttcgcga gtgggcgcgc gcggtgtgtt ggaacacgtt cgaggcaccg
     9361 acgcgccgca agtacaacgc agcgatcatc ttcaagcgtg cgaagggcca aggccgcatc
     9421 tcgcggatca ccgggctcga cgagttcatg cgccagtacg gcgagcacgt cgtcgaatcg
     9481 aagctgctgc cggtcggtgc gcagcgccgc aactggaagc acacgctcgt ctccgacggc
     9541 tacattttgc tgaggcaccc cgattgggcg accgcctgca agctcgccga tatcgtcgcg
     9601 acgcaagtcg agatgtacgc cgagtagctc tacgcttcgt cggcttcgtc gcgcggtgcc
     9661 ggaatatcgt cgatagaagc ggcggtctga tggtcggtat cgatctcgcg atcgaagtcg
     9721 cgctccggca gcgcggccgc gatctgcgcc tcgcgctcgg gcgtgatccg cgccgtcggg
     9781 ccggtctgcg gcagctcgag ctggcggctc ggctcttcgg cgacgacatc gtcggcagcg
     9841 agctcgtgct cgagcagctt cgatgcgatc tcgaggaagt cgccctcggt gacgatgcca
     9901 atgaggcgct cgcggtcgac aaccgggaga cagccgactt gcagccgccg catcatgcgc
     9961 agcgcatcga gcgtcgccgt ctcgggcgat acggtcgtga cctcgcgccg catgatctcc
    10021 gagatcggcg tgcgcagcgt cgagcgacca ctctgcacga agcgcagtac ggcgcggtgg
    10081 ctgacgagac cgacgaggtt gtggtcggag tcctcgacgc agacgtaacg gatgcgttcc
    10141 cagatcatga ggttgacgac catctcgatc gcatcctcgg catggaccgt gaacaggtcc
    10201 gtctccatgt aattctcgac gcgcgcgtag ttgcccttcg acgtgttcgc ctcgtcgagc
    10261 cgtgcgcgct cccactcgga gacggggcgg cgtgcagcct ggcggttgac cgt
//
LOCUS       DATLBZ010000148         8864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_142433, whole genome shotgun sequence.
ACCESSION   DATLBZ010000148 DATLBZ010000000
VERSION     DATLBZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142433"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="VMZ53_07895"
     CDS             <1..1567
                     /locus_tag="VMZ53_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HUS28414.1"
                     /translation="GASLVWYVTIPEGAHLVGEVAGVCHVEVGARSAEGTLAGGMLGA
                     DQDRVDLSAMGGKVVRLSLTARDCPRARVVHPRVTLHGPPVEALPKADPPKLVILWVM
                     DSLRADKIPVFTPGARAQTPNFEELAKSSVVFRQYYVQGNESQTSHSSVWTSLYPAVH
                     GVRLAGQGGTYKIEKSFDVLGDVLTKAGFYTIGVTANGFINADGGYARGFKEFRNMMR
                     EQGPIIIYGETVLAAMYERLDKHRDAPAFVFMGSIDNHYPWIARKPWIDTYSPGYKGP
                     FKLYPTPQDLGFQPGDMGCNITPPPEDIERMRAIYDSDVSYMDQQLGKLVEKLKSWNL
                     WDQTMLIVTADHGEEFFEDGRCGHGGSLRESLIRVPLLIRDPSRFPGGTIVEEGAEGV
                     DILPSILDALGQTPVPAAQGHSLAPLAQGIGRGWPTPSYASMYEYAHAMRIGRWKLRV
                     GATGVPIVEDLVEDPDEKKDFSAVRPIERRMLTDNLGLFLALREQWKKSAWGSVTNTT
                     PVGAAAIDEAAAP"
     gene            1564..2676
                     /locus_tag="VMZ53_07900"
     CDS             1564..2676
                     /locus_tag="VMZ53_07900"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HUS28415.1"
                     /translation="MKRLVVVGLLSCAAGPDIATTPPSRPPPPPPDDAPLVSVAEPDA
                     APVSTLEPPPVVVEDPAATWLRGSTHVHSKPSGDSNVPTPDVEAWYEKHGYDFIVLTD
                     HNRVTVSQTTKVIVIPGIELTMNPQRCEPPGDGTCRIHINGLGVTARPEGKLDWPDRS
                     KHDRIELYAKAIETAQGLGTAVVQINHPNWHWGMTPELLAELGRRGAKLVELENVQFT
                     KWNAGDQDHPSTEAEWDAVLNAGITMWGVASDDAHDYQADGRGKYPAGGGWVVVKARR
                     NPQAILDALAAGKFYSSTGVSLTRADVDHDELVVEVASEPTRTYTITFIENGTPVATV
                     NGLVARRALPSKGYVRAVITRDDGKKAWVQPVRRGN"
     gene            complement(2673..3293)
                     /locus_tag="VMZ53_07905"
     CDS             complement(2673..3293)
                     /locus_tag="VMZ53_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS28416.1"
                     /translation="MIRLALAEDHTIVRWALREALSKADDIEVVGEAGTAAETLSMVQ
                     NVKPDVLLLDITLPDRSGFDVLAELRQVDTAPLVVVLTWHTEPSYAARAIAAGAHGYV
                     NKAVEPNDLLNSIRAVARGEQVIPPGVEQLLASGDGHPASALTAREAQVMEMLARGMT
                     NREIAEHLDISIKTVDTHRGHVLKKLGLRNNSELTRFAVKHGYVSL"
     gene            3369..5411
                     /locus_tag="VMZ53_07910"
     CDS             3369..5411
                     /locus_tag="VMZ53_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28417.1"
                     /translation="MDPAHQNQQLGEFRIEGVLGQGGSGIVYDATWGPRRVALKVLHP
                     SLVGTGKERAQFLTEAQRLQQIMHPSVVKVLALGQLPDGRPYLAMERLDGETLASVIA
                     RGALPLDRALAMFGELCSAVAALHAQGLVHRDLKPENVFVVADRHAVLLDFGIAKDIT
                     APASTTTQEGNVRGTPAYMAPERFFGSPAGIATDVYELALVLYVMLAGRLPWDEVADP
                     EARLSPRPLASLQLAPSVPEPLDTEIRRALSTRAQNRPSGAQALLDAVRAAASGADTD
                     PEPSSTARLRSGAQDAVAPTLANAATAERSERQRAALATTIAAPTTQKRQRAKRSVLL
                     AIGGAIALASGAVAAYKLTRSPSPEPKHAVIPVAPIASDAAVETYDPKDPWSTKPAEP
                     SKAFALVDEKHTPEQYRAEASAAVQKLPNDTRFLFTVQLTELRDNSSTNDLLDKIAKH
                     PKVAPLALILPPCVKGIIADAEWFVFGAPTLEHSSNGTLVLRGRWRRSDVEACFADTV
                     KVYVANDGAKLFRVGDEGWLDFLDDHTAVVTLNTKLEAEALHKLTKKPQGPITRVKQM
                     FAALPANRTIALVSDGKNANEDWSSLSLPKGSDVFGWIRVESSGMAMDLAADPHNTEA
                     AKAAILRIKPDIDNMFLNTSPDTVGKLEVVAQGTVVHVRGRITSLMISLVTASISL"
     gene            5426..6067
                     /gene="udk"
                     /locus_tag="VMZ53_07915"
     CDS             5426..6067
                     /gene="udk"
                     /locus_tag="VMZ53_07915"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HUS28418.1"
                     /translation="MARPLVVGIAGGTGSGKTTVAHKLAAAMPADRVVTIEHDSYYRD
                     QSHLSVDERAKTNFDHPSSLESSLLAEHLRELREGRAVEVPSYDFATHTRRPERRKVV
                     PAPLIIVEGILVFAEAQLRDQMDIKIFVDTDADIRLIRRIRRDIEVRGRSFQSVRDQY
                     YATVRPMHIEYVEPTKRWADLIVPEGGDNHVALDVLLGRLGRIAFGPIVAPKP"
     gene            complement(6070..7047)
                     /locus_tag="VMZ53_07920"
     CDS             complement(6070..7047)
                     /locus_tag="VMZ53_07920"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HUS28419.1"
                     /translation="MRAIQIETTGGPEVMQLADVPTRDPGPGEVRIRHSAVGINFIDT
                     YNRTGLYAAKLPLILGAEGAGVVEAVGPGVPHLTVNDRVAYAARSQGSYAECRTLDAT
                     PVVKLPDAIDFETAAAMMLKGLTVQYLLRRTRAFEPLERGDAIVWHAAAGGVGLIACQ
                     WAATLGYQVIATAGSDEKCALATAHGAAHAINYRREDVVARVKELTNGRGVKVVYDSV
                     GKDTWDISRECLRPFGLLVSFGNASGAVPPQSLLQLSPKSLYVTRPTLNTHTATHEVT
                     QQMADDLFAVVAAKQVKIAIGQRYKLEDAAQAHRDLEARKTTGSSILTL"
     gene            7080..8021
                     /locus_tag="VMZ53_07925"
     CDS             7080..8021
                     /locus_tag="VMZ53_07925"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS28420.1"
                     /translation="MSSPIVVVTGGAGFIGSHTVDRLMESGHRVVVLDNFTTGKRANL
                     SRWANDPKLHVVVCDVAHGIFAALAPVTAKYGPVERIIHLAAQVSVVHSVANPLVDMQ
                     INYGGTLHVLEYARAHNVKKVVLASSAAVYGDTTELPVSEDTKCQPMSPYGIDKYTSE
                     FALDYYASVHSVPTTSLRFFNVYGPRQDPSSPYSGVISIFTDRARAGRPLTIYGDGSQ
                     TRDFVFVGDVVRAIIAASGDGNSRLIANVGTGSEISVLELARSIVELCGTKSTIEHAP
                     ARAGEILKSRARVDRLRDALGVVAETKLVDGLRATLG"
     gene            8051..>8864
                     /locus_tag="VMZ53_07930"
     CDS             8051..>8864
                     /locus_tag="VMZ53_07930"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS28421.1"
                     /translation="MRTGTWLAVVVLAACGGGGKKPAAKPDPAAAAAASSAEPASAST
                     GAAAKAEMPPPMSMAQKMEMTTRGFLTAWNAHEAETVANQYEADAVLRILPGGDMTGR
                     DAIKSQVAETIAGFPDFKAGVSRVVMKGNVSVVEWVVVGTAASKRKIGVHGVSVFTFA
                     DSGLIKEEHRYWDIPTQMAQLDAKAKKGTFRPVADLPSDKPATLSSTGSPDEAKNLEV
                     VNAVYAAIDAHKADEVLKYMDPNGGGDDYTEPASRQGTKGIKEYLGVFFKAVP"
BASE COUNT         1582 a         2886 c         2900 g         1496 t
ORIGIN      
        1 aggggcaagt ctcgtctggt atgtgacgat tcccgaaggc gcgcatcttg tcggcgaggt
       61 cgccggcgtg tgtcacgtcg aggtcggcgc gcgatccgcg gaggggactc tggccggcgg
      121 catgctcggc gccgatcagg atcgcgtcga cttgtcggcg atgggcggca aggtcgtgcg
      181 gctgtcgctg accgcgcgtg actgcccgcg tgctcgcgtc gtgcatccgc gcgtgacgct
      241 gcatgggccg cctgtggagg cgctgcccaa agcggatccg ccgaagctcg tcatcttgtg
      301 ggtgatggat tcgctgcgcg ccgacaagat tcccgtgttc acgcctggcg ctcgcgcgca
      361 gacgccaaac ttcgaggagc tcgcgaaatc gagcgtggtg ttccggcaat actacgtgca
      421 aggcaacgag tcacagacga gccactcgag cgtgtggacg tcgttgtatc cggcggtgca
      481 tggcgttcgg ctcgccgggc agggcgggac gtacaagatc gagaagagct tcgacgtgct
      541 cggcgacgtg ctcacgaagg ccggcttcta cacgatcggc gtcaccgcga acggcttcat
      601 caatgcggat ggcggctacg cgcgaggctt caaggagttt cgcaacatga tgcgcgagca
      661 ggggccgatc atcatctatg gcgagacggt gctcgctgcg atgtacgagc ggctcgacaa
      721 gcatcgcgac gcgccggcgt ttgtcttcat gggctcgatc gacaaccact atccgtggat
      781 cgcgcgcaag ccttggatcg atacgtactc gcctggctac aagggcccgt tcaagctgta
      841 cccgacgccg caagacctcg gctttcagcc cggcgacatg ggctgcaaca tcacgccgcc
      901 gcccgaggac atcgagcgca tgcgtgcgat ctacgacagc gacgtcagct acatggatca
      961 gcagctcggc aagctcgtcg agaagctcaa gagctggaac ctgtgggatc agacgatgct
     1021 gatcgtgacg gcggaccacg gggaggaatt cttcgaggat ggtcgctgcg gtcacggtgg
     1081 gtcgctgcgc gagtcgctca ttcgcgtgcc gctgctgatt cgcgatccgt cgcggtttcc
     1141 cggcggcacg atcgtcgaag aaggggcaga gggcgtcgac atcttgccgt cgatcctcga
     1201 tgcgctcggc cagacaccgg tccctgcggc gcaaggtcac tcgctcgcac cgctcgcgca
     1261 gggcatcggt cgtggctggc cgactccgtc gtatgcatcg atgtacgagt acgcgcacgc
     1321 gatgcgcatc ggccgctgga agctgcgcgt gggtgcgacc ggcgttccga tcgtcgagga
     1381 tctcgtcgag gatcctgatg agaagaagga tttctccgca gtgcggccga tcgaacgacg
     1441 catgctgacg gacaaccttg gattgttcct ggcactacgc gagcagtgga agaagtcggc
     1501 gtggggctcc gtcacgaaca cgacgcccgt gggagctgcg gcgatcgacg aggccgccgc
     1561 accgtgaaac gccttgtcgt cgtcggctta ctgagctgtg cagcgggccc cgacatcgcg
     1621 acgacgccgc catcgcggcc tcctcctccg ccacccgacg acgcgccgct cgtcagcgtc
     1681 gccgagcccg atgccgcgcc ggtgtcgacg ctcgagccgc cgccggtcgt cgtcgaagat
     1741 ccagctgcca cctggcttcg cggctcgacg cacgtccact cgaagccatc cggcgacagc
     1801 aacgttccga cgcccgacgt cgaagcttgg tacgagaagc acggctacga cttcatcgtc
     1861 ctcaccgatc acaaccgcgt gaccgtgtcg cagaccacga aggtcatcgt cattcccggt
     1921 atcgagctga cgatgaaccc gcagcgttgc gagccacctg gagacggcac ctgccgcatc
     1981 cacatcaatg gtctcggcgt tacggcgcgg ccggagggaa agctcgactg gccggatcgc
     2041 agcaagcacg accgcatcga gctgtacgcg aaggcaatcg agaccgcgca gggcctcggc
     2101 accgccgtcg tccagatcaa tcatccgaac tggcattggg gaatgacccc agagctcctc
     2161 gcagagctag gtcgacgtgg tgcgaagctc gtcgagctcg agaacgtgca gttcacgaaa
     2221 tggaacgccg gcgatcagga tcatccatcg accgaggccg aatgggatgc tgtcctgaac
     2281 gccggcatca cgatgtgggg cgtagcgtcc gacgacgcgc atgactacca agctgacggt
     2341 cgcggtaagt atcctgcggg cggaggttgg gtcgtcgtaa aggcacgacg caacccgcag
     2401 gcgattctcg acgcactggc ggctggtaag ttctactcgt cgacgggcgt atcgctcact
     2461 cgtgcggatg tagaccacga cgagctagtc gtcgaagtcg cttccgaacc aacgcgaacg
     2521 tacacgatca cgttcatcga gaacgggacg ccagttgcta cggtgaacgg gcttgttgcc
     2581 cgacgcgctc ttccttcaaa ggggtatgtc cgcgcggtca tcacgcgaga cgacggaaag
     2641 aaggcgtggg ttcaacccgt tcgccgcggc aactagagcg acacgtaccc gtgcttgacc
     2701 gcgaagcggg tgagctcgga gttattgcga agccccagct tcttcagcac gtgaccgcga
     2761 tgggtatcga cggtcttgat gctgatgtcg aggtgctctg caatctcgcg attggtcatg
     2821 ccacgcgcga gcatctccat cacctgagct tcacgagccg tgagcgcgct cgccggatgt
     2881 ccgtcgcccg aagcgagaag ctgttccaca ccgggtggaa tcacttgctc gccgcgcgca
     2941 acggcccgga tcgagttcag caggtcgtta ggctcgaccg ccttgttcac gtaaccgtga
     3001 gcaccggcag caatcgcgcg cgcggcgtaa ctcggctcgg tgtgccacgt gagcaccacg
     3061 acgagaggcg ccgtgtcgac ttgccggagc tcggcgagaa cgtcgaaccc ggaacggtcg
     3121 ggcagggtga tatcgagaag aagcacgtcg ggtttgacgt tctgcaccat ggacagcgtc
     3181 tccgctgcgg tgcctgcttc tccaacgact tcgatgtcat cagccttgct gagtgcttct
     3241 ctcaaggccc aacgaacgat ggtgtggtct tcagctagtg cgagtcggat cattgcgtcc
     3301 tccgctgctc cgacaaagta gcaacccccg ggccggttca aggcgggtta gggtgagggt
     3361 gctcttccgt ggatcccgca caccaaaacc agcaactcgg tgagttccgc atcgagggcg
     3421 tacttggcca aggcggcagc ggaatcgtct acgacgccac gtggggaccc cgtcgggtcg
     3481 cactaaaagt tttgcatccg agccttgtgg gcacaggcaa ggagcgcgcg cagttcctca
     3541 ccgaggccca gcggctgcaa caaatcatgc acccgtcggt ggtgaaggtc ctcgccctcg
     3601 ggcaattgcc tgatgggcgc ccgtacctcg ccatggaacg cttggacggc gagacgctcg
     3661 cgagcgtgat cgcgcgcggt gcgctgccgc tcgatcgcgc tctcgcgatg tttggcgagc
     3721 tttgttcggc cgtcgcggcg ctccacgcgc agggactcgt gcatcgcgat ctcaaaccgg
     3781 agaacgtgtt cgtggttgcg gatcggcacg ctgtgctcct cgacttcggg atcgcgaaag
     3841 acatcaccgc gcccgcatcg accactacgc aagaagggaa cgtgcgcggt acgccggcgt
     3901 atatggcgcc ggagcgattc ttcggctcgc cggcggggat cgcgacggac gtctacgagc
     3961 tcgcgctcgt gctctacgtg atgctggcgg gtcgactgcc gtgggacgag gtcgccgatc
     4021 ccgaagcgcg tctgtcgccg cgtccgctcg cctcgttgca gctcgcgccg tccgtgcccg
     4081 aaccgctcga caccgagatt cgtcgcgcgc tgtcgacacg cgcgcagaat cgcccatcgg
     4141 gcgcgcaagc attgctcgat gccgtacgcg ccgccgcgag cggagctgac acggatcccg
     4201 agccttcgtc gaccgcgcgt cttcgctcgg gcgcacagga tgcggttgct ccaacgctcg
     4261 cgaacgccgc gactgcggag cgcagcgagc gacagcgcgc cgcacttgcg acgacgatcg
     4321 cagcgccgac cacgcagaag cgtcagcgcg cgaagcgatc ggtgctgctc gcgatcgggg
     4381 gagcgatcgc tctggcgagc ggcgctgtgg cggcatataa gctcacgcgg tcaccatcgc
     4441 ccgaaccaaa gcacgccgtc atcccagtcg cgccgatcgc cagtgatgcg gctgtcgaga
     4501 cctacgaccc gaaggatccg tggagcacga agcccgcgga gccatcgaag gcgttcgcgc
     4561 tcgtcgacga gaaacacacg cccgagcaat accgcgcgga ggcgagcgct gctgtccaga
     4621 agctgccgaa cgacacgcgc tttctgttca ccgtgcagct caccgagctt cgcgacaaca
     4681 gctcgacgaa cgatctactc gacaagatcg cgaagcatcc gaaggtcgcg ccgctcgcgc
     4741 tgatcttgcc gccgtgcgtg aagggcatca tcgcggacgc ggagtggttc gtgttcggcg
     4801 cgccaacgct cgagcactca tcgaacggca cgctcgtgct ccgcgggcgc tggcggcgca
     4861 gcgatgtcga agcgtgcttc gccgacaccg tcaaggtcta cgtcgcgaac gacggcgcga
     4921 agctgttccg cgtcggcgat gaaggctggc tcgacttcct cgacgatcac acggcggtgg
     4981 tcactctcaa cacgaagctc gaggccgagg cgcttcacaa gctcacgaag aaaccgcagg
     5041 gaccgatcac gcgcgtgaag cagatgttcg ccgcgctgcc cgcgaaccgc acgatcgcac
     5101 tcgtctccga cggcaagaac gcaaacgagg attggtcgtc gctgtcgctg ccgaagggct
     5161 ccgacgtgtt cggctggatc cgcgtcgaga gctccggcat ggcgatggac ctcgccgccg
     5221 acccgcacaa caccgaggct gcgaaggccg cgatcctgcg catcaaaccg gatatcgaca
     5281 acatgttcct caacacgagc cccgacacgg tcggcaagct cgaagtggtc gcgcagggca
     5341 cggtcgtcca tgtccgcggc cgcatcacct cgctgatgat cagcctggtg acagcgagca
     5401 tcagtttgtg agatagacgc ggccaatggc acgtccgctc gtcgtcggta ttgccggtgg
     5461 tactggctcg ggcaagacca ccgtcgcgca caagctcgct gcggcgatgc cggcggaccg
     5521 cgtcgtcacg atcgagcacg actcgtacta tcgcgaccag agccacctct cggtcgacga
     5581 gcgcgcgaag acgaacttcg atcatccgtc gtcgctcgag tcgtcgctcc tcgcggagca
     5641 tctgcgcgag ctgcgcgaag gccgcgcggt cgaggtccct agctacgact tcgcgacgca
     5701 cacgcgccgc cccgagcgcc gcaaggtcgt tcccgctccg ctgatcatcg tcgagggcat
     5761 cctcgtgttc gccgaagcgc agctgcgtga tcagatggac atcaagatct tcgtcgacac
     5821 cgacgcggac atccgcctga tccgccgcat ccggcgcgac atcgaggtgc gcggccgctc
     5881 gttccaatcc gtgcgcgacc agtactacgc gacggtgcgg ccgatgcaca tcgagtacgt
     5941 cgagccaacg aaacggtggg ccgacctgat cgtccccgag ggtggcgaca atcacgtcgc
     6001 gctcgatgtc ctgctcggtc ggctcggccg gatcgccttt ggcccgatcg tcgcgccgaa
     6061 gccgtagcgc tagagcgtga gaatcgacga gcccgtcgtc ttgcgcgctt cgagatcgcg
     6121 gtgcgcttgc gccgcatcct cgagcttgta gcgctggccg atcgcgatct tcacctgctt
     6181 cgcagcgacg accgcgaaca gatcatcggc catctgctgc gtgacctcgt gcgtcgccgt
     6241 gtgcgtgttg agcgttggcc gcgtcacgta cagcgacttc ggcgagagct gcagcaggct
     6301 ctgcggcggc acggcgccgg atgcattgcc gaagctgacg agcaggccga atggccgcaa
     6361 gcactcgcgc gagatgtccc acgtgtcctt gccgacggag tcgtacacga ccttcacgcc
     6421 acgaccattc gtcagctcct tgacgcgcgc cacgacatcc tccctgcggt agttgatcgc
     6481 gtgcgcggcg ccatgcgcgg ttgcgagcgc gcacttctcg tccgagcccg cggtggcgat
     6541 cacctggtag cccagcgtcg cggcccactg acacgcgatg agcccgacac cacccgcggc
     6601 cgcgtgccac acgatcgcgt caccacgctc gagcggctcg aacgcgcgcg tgcggcgcag
     6661 caggtattgc accgtgaggc ccttcagcat catcgcggcg gcggtctcga agtcgatcgc
     6721 atccgggagc ttcacgaccg gcgtcgcatc gagcgtgcgg cactccgcgt agctgccctg
     6781 gctgcgggcg gcgtaggcca cacgatcgtt caccgtgaga tgcggaacgc ctggcccgac
     6841 cgcttcgacc acgccggcgc cctcggcgcc gagaatcagc gggagcttcg cggcatagag
     6901 cccggtgcgg ttgtacgtgt cgatgaaatt gatgccgacg gcgctgtggc gtatgcgcac
     6961 ttctcccggg ccgggatccc gcgttggcac gtcggccagt tgcatgactt caggaccgcc
     7021 ggttgtttcg atctggatcg cgcgcatgcg cgtcgcacgc tagcaggtac actgcagacg
     7081 tgtcctcccc catcgtcgtc gtcaccggcg gagccggttt cattggctcg cacaccgtcg
     7141 atcggctcat ggagtccggt catcgcgtag tcgtcctcga caacttcacg accggcaagc
     7201 gcgcaaacct ctcgcggtgg gcgaacgatc cgaagctgca cgtcgtcgtc tgcgatgtcg
     7261 cgcacggcat cttcgctgcg ctcgcaccgg tcaccgcaaa atacggtccc gtcgagcgca
     7321 tcatccatct cgccgcgcag gtgtcggtcg tccactcggt cgcgaacccg ctcgtcgaca
     7381 tgcagatcaa ttacggcggc acgctgcacg tcctcgaata cgcgcgcgca cacaacgtga
     7441 agaaagtcgt gctcgcatcg tccgccgcgg tctacggcga caccaccgag ctgccggtct
     7501 cggaagacac gaagtgccag ccgatgtcgc cgtacggcat cgacaagtac acgtcggagt
     7561 ttgcgctcga ctactacgcc agcgtgcact ccgtcccaac gacgtcgctg cgcttcttca
     7621 acgtctacgg cccgcgccaa gatccgtcga gcccctactc gggcgtcatc tcgatcttca
     7681 ccgatcgcgc acgcgccggt cggccgctca cgatctacgg cgatggctcg cagacgcgcg
     7741 acttcgtctt cgtcggcgat gtcgtccgcg cgatcatcgc tgccagcggc gatggcaact
     7801 cgcgtttgat cgcgaacgtc ggcacgggca gcgagatcag cgtgctcgag ctggcgcgca
     7861 gcatcgtcga gctgtgcggc acgaagtcga ccatcgagca cgcgcccgct cgcgccggcg
     7921 agattctgaa gtcccgcgca cgcgtcgatc gtctgcgcga tgcgctcggc gtcgtcgctg
     7981 aaaccaagct cgtcgacggc ttgcgcgcca cgctgggctg atctacgaag ggcctcaaaa
     8041 gggaggctct atgcggacgg gtacatggtt ggcggtagtg gtgttggcgg cgtgtggtgg
     8101 tggtgggaag aagcctgcgg cgaagccgga tccggcggca gcggcagcag cgtctagtgc
     8161 cgagccggcg tcggcatcga caggtgcagc ggcaaaggcc gagatgccgc cgccgatgtc
     8221 gatggcgcag aagatggaga tgacgacgcg cgggttcctg acggcgtgga atgcacacga
     8281 agcggagacc gtcgcgaatc agtacgaggc cgatgcggtg ctgcgcatcc taccgggcgg
     8341 cgacatgacc gggcgtgatg cgatcaagag tcaggtcgcc gagacgatcg ccggattccc
     8401 ggatttcaaa gctggcgtca gccgcgtcgt gatgaaaggc aacgtctcgg tcgtcgagtg
     8461 ggtggtcgtt ggaaccgcgg cgtccaagcg caagatcggc gtgcacggcg tgtcggtgtt
     8521 cacgttcgcc gactccgggc tgatcaagga agaacatcgt tactgggaca ttccgaccca
     8581 gatggcgcag ctcgacgcga aggcgaagaa gggcacgttc cgcccggttg ccgacctgcc
     8641 gagcgacaag ccagcgacgc tctcgtcgac gggctcgccc gatgaagcga agaacctcga
     8701 agtcgtcaac gcggtgtacg cggcgatcga cgcccataag gcggacgagg tcctgaagta
     8761 catggatccg aacggtggcg gcgatgacta caccgagccg gcgtcgcgtc agggtacgaa
     8821 gggcatcaag gaatacctcg gcgtgttctt caaagcggtg ccgg
//
LOCUS       DATLBZ010000149        11179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1633545, whole genome shotgun sequence.
ACCESSION   DATLBZ010000149 DATLBZ010000000
VERSION     DATLBZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633545"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VMZ53_07935"
     CDS             <1..964
                     /locus_tag="VMZ53_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HUS28422.1"
                     /translation="AYVLDIDRNARGPQIILSRTHKGLLEKLFEQEVPEIYEKIVRIE
                     SSAREPGARAKIAVSSRDRDVDPVGACVGMKGSRVQAVVQELRGEKIDIVPYDDDPAR
                     FVCNAIAPAEVKRVIIDAEGHRMELIVPDDKLSLAIGKKGQNVRLASQLTGWRIDIHS
                     ESKIYELERRAKEQIGQAIAGEGFDNAVSETLFKLGWRSVGELARAHVEELAGLPGIG
                     GVDGAKKIIEGAKGYMQSGGRRKDDARKEAERRSSMSGDQQLLELDGVDEDVLNVLHS
                     ANVHSPEDLIKTPIEQIATTTGIDMGDLARLRQRAISWLSEVSS"
     gene            1307..4408
                     /gene="infB"
                     /locus_tag="VMZ53_07940"
     CDS             1307..4408
                     /gene="infB"
                     /locus_tag="VMZ53_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HUS28423.1"
                     /translation="MYEIAKEVGIPNKDLIAKIRALGLEVNNHMSSLDADDVARIKRS
                     LEKEKTGGQAPAQTQRLSTGGAVLRRRSANDKPEGEPEARPSTPVAAAPAVPPPVIRR
                     RVAAEEAPAPAPVKPQPVEAPKQEATPPPAVMKRMPAATPPPEPQAEPARPAPAKVES
                     PTPAPQPTVTKRAPVAPPVETPAPAPVVEARVEAPAPAPRPPAPKPVEAPVAAAAAAA
                     SEAPAPEAQQPAARSRVIVNGPQIVSRPSPTQGNGQKQHATEPQRAPEPPPSTPLPPR
                     IAPVRVERRDNDGRSGPIVEHRNPGGPRTVEEVAGTARSRFELELERARARSADRPRP
                     DTGRIEPQPAVPEGQPEEVVAAPPTRDPSRPAVGSVIALPPRIKITERTPMAGRGPAP
                     GPQPVNPIRGRFAQQQQQRGPRPGGHGRPGGPNDMMGRKKLPLGKKGKQTQLTTPAEH
                     KRVIRIEDSITVSDLARGMGIKAPEVLKKLWGMGMTGVNINAAIDFDTAQLLAGEFAY
                     EVQNVAFKEDDIFTQKTDEATDMHLRAPVVTVMGHVDHGKTTLLDSIRKARVAAGEAG
                     GITQHVAAYKVPSTHGDIVFLDTPGHEAFTKMRARGAQATDIVVLVVAANDGVMPQTV
                     EALSHAKDAKVAIIVAVNKIDMPDAAPERVKQQLADHGLIPEEWGGETMYVNVSAIKG
                     ENIDKLLEAVSLTAEVLELRANPNKPASGLVIEARLDRNRGPMATVLIQEGTLRTGDV
                     LVAGRTFGKVRAMLDDRGQTVEEAGPSTPVEILGLDGVPDAGDQVNAAEDDKVAKQVV
                     EHRRQQFRKRELASNSRISLEGLMERNSVDAIKDLKIVLKADVQGSAEALKAALTKLA
                     TDKVKVSVIAAAVGGITESDVNLAKAGGAIIVGFHVRPAGKSSKLAEQEGVQIRLYDI
                     IYDALDEVKGAMAGLLAPIKREVAMGKLQVRETFSIPKIGTVAGCMVTEGKINRKAHL
                     RIIRDAVQVYEGKIGSLRRFKDDVSEVQHGFECGVMVAGWNEIKSGDIIEAYEVVEEA
                     AQL"
     gene            4488..4895
                     /locus_tag="VMZ53_07945"
     CDS             4488..4895
                     /locus_tag="VMZ53_07945"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HUS28424.1"
                     /translation="MYVGVAKLSLVIGDAHSLKEKRMVLRRIKDRVRERLHVTVNEVG
                     ELDSWQRAELGCAVVSGDRTKALELIDDVVRVATSSGGGEIVAVAKTVSTFDAESLPF
                     APIDERTGSGDKAQGQGDADWIPDEWRDEAHKS"
     gene            4892..5341
                     /gene="rbfA"
                     /locus_tag="VMZ53_07950"
     CDS             4892..5341
                     /gene="rbfA"
                     /locus_tag="VMZ53_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HUS28425.1"
                     /translation="MSPTKKSAGGSGSGRKTRVEHAMREALTEAIAADIRDPRVHAAS
                     MLTVTKVELNVDMSVARVYISIVGDDATADAALLGLEKAAGFLRGPIGRRLGLQHAPE
                     LRFMMDPSLDMSDKLAAILREDEEKAKAAGRDLKAEAAGPEVKKGES"
     gene            5338..6447
                     /locus_tag="VMZ53_07955"
     CDS             5338..6447
                     /locus_tag="VMZ53_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HUS28426.1"
                     /translation="MRDGGDGYDRRQTEPMTTHDVGAALAKACEKLRASQRILLTSHR
                     RPDGDGTGSMAGLASFLRALGKEAVIYSVDHIARRYKWLPLVNTTVHTIGFDERFDCT
                     IVVDCADISLLGDTLPPPEVCGTLITLDHHASGHPFGDIAVWDPTAAAVGVLVHRIAV
                     AEGWPVTLEAAVPLYVSLISDTGGFRHANTNGEALRVGSDLINAGVIPSAIAASLEER
                     PSPGKLRLLGSVLSTLELQCAGRVGVLSVTTDMVEGARASWEDIEGMVNWARNVEGVQ
                     VGVLLTTAKGGGVRVSMRSRSEKIDVGRVCMTLGGGGHPGAGGCHMNGDLSTTKDKVV
                     RALETAFNRISQPVIRREEKPTLETPIAGPDKADT"
     gene            6463..7518
                     /locus_tag="VMZ53_07960"
     CDS             6463..7518
                     /locus_tag="VMZ53_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28427.1"
                     /translation="MRWSLPVLMALVACGDDGATTKMDAMVDSPSVMGDVPVDGKDPN
                     NPLTLADTGLCLDAGCTQISTDVYAFQPQFQLWSDAATKKRWIYLPPGTQINTANMDF
                     WEFPVGTKLWKEFTRDNVRVETRLVMRIGNAGMPSDWFYVPYVWNASQDATTAQTAGV
                     QNANGTQHDVPSRFDCKSCHENVKPTRILGFSAIQLDWDNPDAAQLDLKELVAKNLLT
                     NPPAAPSTVGAPYFPLPGTTYEPPALGYLHANCGHCHNPTSGNYGATPMELRLTVATL
                     GALSTTPAYTTAVNCNTNVGGTTATKIIVAGQPSMSAMIERFESTNPTVHMPQKGSEV
                     MDPTGDTTLKTWITNIP"
     gene            7520..8389
                     /gene="truB"
                     /locus_tag="VMZ53_07965"
     CDS             7520..8389
                     /gene="truB"
                     /locus_tag="VMZ53_07965"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HUS28428.1"
                     /translation="MHGVLVLDKPRDMSSASAVEHVRRRLGIKRAGHGGTLDPIATGV
                     LAICLGEATKLAGYLLADDKEYEADLVLGSETDTYDRTGVVTSTRPADHITAEMIERV
                     LAARTGEQDQLPPIYSAIKQDGVRMYHRARRGETLETIERTPRRIRIDRLALLDYTAP
                     VARIAIACSKGTYVRSIVADIGTDLGVGAHMSELRRTRSGTFTLAQAHDLDRMDNCRI
                     VPLEQATKLPIVIASDDHITKIRNGLQMPPDVLGVAPEIFQKFQLFNEARKLVAVAHV
                     DRNRVIYDRVFAP"
     gene            8474..8749
                     /gene="rpsO"
                     /locus_tag="VMZ53_07970"
     CDS             8474..8749
                     /gene="rpsO"
                     /locus_tag="VMZ53_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HUS28429.1"
                     /translation="MPTSISTVRKQDTIGKFRQHGTDTGSPEVQVALLSERIAHLTEH
                     LKTNHKDHHSRRGLLKMVGQRRALLDYLKRKSLDRYKKLIETLGLRR"
     gene            8792..9184
                     /locus_tag="VMZ53_07975"
     CDS             8792..9184
                     /locus_tag="VMZ53_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28430.1"
                     /translation="MQGTVGYVLIAVGVIVGFLGTARSERAAARDPDGRDRMLGALRK
                     AKLAVVVIAVAIALYLAMRHYGHGRLGLQICTAILGVNATAVAVLKMRAAGEHLSGDN
                     RATFKRFAIAEAIGYILVFAGIYLYLTR"
     gene            9188..9700
                     /locus_tag="VMZ53_07980"
     CDS             9188..9700
                     /locus_tag="VMZ53_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28431.1"
                     /translation="MRRIASVVVRSLLLSCACNGSSKPTHPVAPPTDAATRNDDDALG
                     AIRNDNIEYATYTRPDDRRKVPPGLYRQVSVTAPQEAFALTRSGDMRAFDLLVPMLDD
                     PERNWAAFVMLAALTGHEAATVIAFDNAAGFKGSAGERDARKNWEAWLADHRATLTWD
                     AERHQFRTAQ"
     gene            complement(9719..10489)
                     /locus_tag="VMZ53_07985"
     CDS             complement(9719..10489)
                     /locus_tag="VMZ53_07985"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HUS28432.1"
                     /translation="MTLETATTPQGGVLDVPAGWRQGAGAYGGLVVASMMRAAEQAIG
                     DAARTIRSVTAEIPAPVEPGRAELTVEVLRAGKSVTAARVALVQAGETRAHAVVIAAT
                     TRTSALAWNSLMPPHAPAWEALKPMPTSGPFPEFAQHFEFRVVEGIPTSGVAGPAIGW
                     IRARAPGRKRDAAYLAAMIDAWFPVALVHAKQPHPMATIAFTLEVTGDASDVGDEALL
                     YRGVSPVCTDGYSFETRELWTASGRLLARNHQTFVIIK"
     gene            complement(10507..10881)
                     /locus_tag="VMZ53_07990"
     CDS             complement(10507..10881)
                     /locus_tag="VMZ53_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28433.1"
                     /translation="MKTDTPGFLDVKLTVVTKEPTAWLDGLATLGEVEADEQNGDWQI
                     RRIKTAAQLRGMTIRAIGYVGPPTADVTVGWVGSDRIVSDGTTEIPDVSIAVVPRVTG
                     APGKETLQTALKEVLREAAKST"
BASE COUNT         2069 a         3733 c         3609 g         1768 t
ORIGIN      
        1 ggcgtacgtc ctcgatatcg atcgcaacgc gcgtggcccg cagatcatcc tcagccgcac
       61 gcacaagggc ctcctcgaga agctgttcga gcaagaggtg ccggagatct acgagaagat
      121 cgtccgcatc gagtcgagcg cacgtgagcc cggtgctcgc gcgaagatcg ccgtctcgtc
      181 tcgcgatcgc gacgtcgatc cagttggcgc atgcgtcggc atgaagggca gccgcgttca
      241 ggccgtcgtg caggagctgc gcggcgagaa gatcgatatc gttccgtacg acgacgaccc
      301 ggctcgcttc gtctgcaacg cgatcgcgcc cgccgaggtc aagcgcgtca tcatcgacgc
      361 cgaaggccat cgcatggagc tgatcgtccc cgacgacaag ctctcgctcg cgatcggcaa
      421 gaagggtcag aacgttcgcc tcgcgtcgca gctcacgggc tggcgcatcg acatccactc
      481 ggagtcgaag atctacgagc tcgagcgtcg cgcgaaggag cagatcggcc aggcgatcgc
      541 cggcgaaggc ttcgacaacg ccgtctccga gacgctgttc aagctcggtt ggcgctcggt
      601 cggcgagctc gcgcgtgcac acgtcgagga gctggcgggc ctgccgggca tcggcggcgt
      661 cgacggcgcg aagaagatca tcgaaggcgc gaagggctat atgcagagcg gcggccgtcg
      721 taaggacgac gcgcgcaagg aagcagagcg ccggtcgtcg atgtcgggcg accagcagct
      781 cctcgagctc gacggcgtgg acgaggacgt actgaacgtc ctccactccg cgaacgtcca
      841 ctcgcccgag gatctcatca aaacgccgat cgagcagatc gcgaccacca ccggcatcga
      901 catgggtgac ctcgcgcggc tccgccagcg tgcgatcagc tggttgtcgg aggtttcctc
      961 ctgattctgc ggggacatat cccgcagcga acctgcgtgg ggtgtcgggc ggtcgagccc
     1021 aaaggcgagc tcgtgcgcgt ggggtgggtc gggccgcacc tggtgattgg ccgggttgcg
     1081 gggcggggtg cgtacgtgca ccgcagtcaa gcgtgttttg acttggccgt tcgaggggga
     1141 tttgcgcggt cgtttcgccg atctatcccc cctgacgaga tccgcaactt cgcgaaaaac
     1201 gtagctggct tggacggctc gaaagctgta gaaactccat ctcctccggc aggcaacatg
     1261 cctgtctgag cgagacgcga gaaggatgac cggagagaag atgcgagtgt atgagattgc
     1321 taaagaggtc gggatcccca acaaggacct catcgccaag atccgcgcgc tcggactcga
     1381 ggtgaacaat cacatgtcgt cgctcgatgc ggacgatgtc gctcgtatca agcgctcgct
     1441 cgagaaggag aagaccggcg gtcaagctcc ggctcagacg caacgtctct ccaccggtgg
     1501 tgccgttctt cgtcgccgct ctgcgaacga caaacccgaa ggcgagcccg aagcgcgtcc
     1561 ttcgacgccg gtcgctgcag cacccgccgt accgcctccg gtgattcgtc gccgcgttgc
     1621 tgcggaagaa gcaccggcgc cggctcccgt gaagccgcaa ccggtcgaag caccgaagca
     1681 agaagcgact cctccgccgg ccgtgatgaa gcgtatgccg gccgcaacgc caccaccgga
     1741 accgcaggcc gagcccgctc gtccagcgcc cgcgaaagtc gagtctccga cgcccgctcc
     1801 gcagcccacc gttacgaagc gcgctccagt cgcgcctcct gttgaaacgc ctgctccggc
     1861 gcccgtcgtc gaagcgcgtg tcgaagctcc tgcgcctgct ccgcgtccgc ccgcgccgaa
     1921 gcccgtcgag gctccggtcg ccgccgccgc cgccgctgcg agcgaagcac ccgctccaga
     1981 agcgcagcaa cccgccgcgc gctctcgcgt gatcgtcaac ggtccgcaga tcgtgtcgcg
     2041 tccgtcgccg acccaaggca acggccagaa gcagcatgcg accgaaccgc agcgcgcacc
     2101 ggagccgccg ccgtcgacgc cgctgccccc tcgcatcgcg cccgttcgcg tcgagcgccg
     2161 cgacaacgac ggccgcagcg gtccgatcgt cgagcacagg aatccgggcg gtcctcgcac
     2221 cgtcgaggaa gttgccggca cggcacgcag ccgcttcgag ctcgagctcg agcgtgcacg
     2281 cgctcgcagc gccgatcgtc cgcggccgga caccggtcgc atcgagccgc agccagcagt
     2341 acccgaaggt cagcccgaag aggttgtagc agcaccgccc acgcgcgatc cgtcgcgtcc
     2401 tgcggtcggc tccgtcattg ctctgcctcc gcgcatcaag atcaccgagc gcacgccgat
     2461 ggcgggccgt ggtcctgctc cgggtccgca gcccgtaaat ccgatccgcg gtcgcttcgc
     2521 gcagcagcaa cagcagcgcg gtccgcgccc gggtggtcat ggtcgtccgg gtggcccgaa
     2581 cgacatgatg ggccgcaaga agctgcccct cggtaagaag ggcaagcaga cgcagctcac
     2641 cacgcctgcc gagcacaagc gcgtcatccg catcgaggac tcgatcaccg tgtcggacct
     2701 cgctcgcggc atgggcatca aggcgcccga ggtgctgaag aagctctggg gcatgggcat
     2761 gaccggcgtc aacatcaacg ccgcgatcga cttcgacacc gctcagctgc tcgcgggcga
     2821 gttcgcctac gaagtgcaga acgtcgcctt caaggaagac gacatcttca cgcagaagac
     2881 cgacgaagcg acggacatgc atctgcgtgc gccggtcgtc accgtcatgg gtcacgtcga
     2941 ccacggcaag accacgctgc tcgactcgat ccgcaaggcg cgcgtcgccg cgggtgaagc
     3001 cggtggcatc acccagcacg tcgcggccta caaggtccca tcgacgcacg gcgatatcgt
     3061 cttcctcgat acgcccggcc acgaagcgtt caccaagatg cgcgctcgcg gcgctcaggc
     3121 aacggacatc gtcgtgctcg tcgtcgctgc caatgacggc gtgatgccgc agacggtcga
     3181 agcgctctcg cacgcgaagg acgcgaaggt cgcgatcatc gtcgcggtca acaagatcga
     3241 tatgcccgac gccgctcccg agcgcgtcaa gcagcagctc gccgaccacg gcctcattcc
     3301 cgaggagtgg ggtggcgaga cgatgtacgt caacgtctcg gccatcaagg gcgagaacat
     3361 cgacaagctg ctcgaggcgg tctcgctcac cgccgaggtg ctcgagctcc gcgccaatcc
     3421 gaacaagccg gcttccggcc ttgtcatcga agcgcgtctc gatcgcaacc gcggtccgat
     3481 ggcgaccgtc ctcatccaag agggcacgct gcgcacgggt gacgttctcg tcgccggtcg
     3541 cacgttcggc aaggttcgcg cgatgctcga tgatcgcggt cagaccgtcg aagaagctgg
     3601 tccgtcgacg cccgtcgaga ttctcggtct cgatggcgtg cccgacgccg gcgatcaggt
     3661 gaacgcggcc gaggacgaca aggtcgccaa gcaggtcgtc gagcaccgcc gccagcagtt
     3721 ccgcaagcgc gagctcgcat cgaactctcg catctcgctc gaaggtctga tggagcgcaa
     3781 ctccgtcgac gcgatcaagg atctcaagat cgtcctcaaa gcggacgttc agggctctgc
     3841 ggaagcgctc aaggctgcgc tcacgaagct cgccaccgac aaggtcaagg tctccgtgat
     3901 cgcggcagca gtcggcggca tcacggagag cgacgtcaac ctcgcgaagg cgggtggcgc
     3961 gatcatcgtg ggcttccacg ttcgtccggc cggtaagagc agcaagctcg ccgagcagga
     4021 aggcgtgcag attcgcctct acgacatcat ctatgatgct ctcgacgagg tgaagggcgc
     4081 catggcgggc ctactcgcgc cgatcaagcg cgaagtcgcg atgggcaagc tgcaggttcg
     4141 cgagacgttc tcgattccga agatcggaac cgtcgcgggc tgcatggtca ccgagggcaa
     4201 gatcaaccgg aaggcgcacc tgcgcatcat ccgggacgcg gtccaggtct acgaaggcaa
     4261 gattggctcg ctgcgccgct tcaaggacga cgtcagcgag gtccagcatg gcttcgagtg
     4321 cggcgtcatg gtcgcgggtt ggaacgagat caagagcggc gacatcatcg aggcctacga
     4381 ggtcgtcgaa gaagccgcgc agctctgagc tccgagcgat ctagctctcc gttcaccctc
     4441 acgatcgaga tgacccgaca agccccgcca tttggtaggg tgtggtcgtg tacgtcggag
     4501 ttgcgaagct gagcctcgtg atcggcgatg cccattccct caaagaaaag cggatggtgc
     4561 ttcgccggat caaagatcgc gtgcgcgagc ggctccacgt caccgtcaac gaagtgggcg
     4621 agctcgatag ctggcaacgc gccgagctcg ggtgcgccgt cgtgagcggc gatcggacga
     4681 aggctctcga gctgatcgac gacgtcgtgc gcgttgcgac ttcgtcgggc ggtggtgaga
     4741 tcgtcgcagt tgccaagacg gtgtcgacgt tcgacgccga gtccctgccg tttgcgccga
     4801 tcgacgaacg cacgggctct ggagacaagg cgcaagggca gggcgacgcc gactggattc
     4861 cggacgagtg gcgcgacgag gcgcacaagt cgtgagcccc acgaagaagt ccgccggcgg
     4921 aagcggcagc ggtcgcaaga cgcgagtcga gcacgcgatg cgcgaagcac tcaccgaagc
     4981 gatcgcagcg gacattcgcg acccgcgcgt tcacgccgca tcgatgctga ccgtcacgaa
     5041 ggtcgagctc aacgtcgata tgtccgtagc gcgcgtctac atctcgatcg tcggagatga
     5101 cgcaacggcg gatgccgcac tcctcggcct cgagaaagcc gctggcttct tgcgcggacc
     5161 gatcggccgg cgactcggtc tgcagcatgc gcccgagctt cgcttcatga tggatccgtc
     5221 gctcgacatg agcgacaagc tcgccgcgat cctccgcgag gacgaagaga aggcgaaggc
     5281 agccggccgc gatctgaaag cggaagccgc tggtccagag gtgaagaagg gcgagtcatg
     5341 agagacggcg gcgacggcta cgaccgcagg caaaccgagc ccatgacgac gcacgacgtc
     5401 ggtgccgcgc tcgcgaaagc ctgcgagaag ctccgcgcgt ctcagcgcat tctgctcacg
     5461 agccaccggc gtcctgatgg cgacggcact ggatcgatgg cgggtctcgc gtcgttcctg
     5521 cgcgcactcg gcaaggaagc cgtcatctac tcggtcgatc acatcgcgcg ccgctacaag
     5581 tggctgccgc tcgtgaacac caccgtgcac acgatcggtt ttgacgagcg attcgattgc
     5641 acgatcgtcg tcgactgcgc ggacatctcg ctgctcggtg acacgctgcc gccacccgaa
     5701 gtctgcggaa cgctgatcac gctcgatcat catgcgagtg gtcatccctt cggcgacatc
     5761 gccgtgtggg atccgaccgc ggccgcggtt ggcgtgctcg tgcatcgcat cgcggtggcc
     5821 gaaggctggc cggtgacgct cgaggctgca gtgccgctct acgtgtcgct gatcagtgac
     5881 accggcggct ttcgccacgc caacacgaac ggcgaagcgc tgcgcgtcgg ctcggatctg
     5941 atcaacgcgg gcgtcattcc gtcggcgatc gcggcgtctc tcgaagagag gccgtccccc
     6001 ggcaagctgc gcctcctcgg ctcggtgctc tcgacgctcg agctgcagtg cgccggtcgc
     6061 gtcggtgtgc tgagcgtcac gaccgacatg gtcgagggtg cgcgcgcctc gtgggaagac
     6121 atcgagggca tggtgaactg ggcgcgcaac gtcgaaggcg tgcaggtcgg cgtgctgctc
     6181 acgaccgcca agggcggcgg cgtccgcgtg tcgatgcgat cgcggagcga gaagatcgat
     6241 gtcggaagag tttgtatgac gctcggtggt ggcggccatc ccggcgccgg cgggtgccac
     6301 atgaacggcg atctctcgac gacgaaggac aaagtcgtgc gtgcgctcga gactgcgttc
     6361 aatcgcatct cgcagcccgt cattcggcgc gaggaaaagc cgacactcga gactccaatc
     6421 gcgggccccg ataaagccga cacctgacgt acaatcggcc gtatgcggtg gagcttgcct
     6481 gtgctgatgg cactggtcgc gtgcggtgac gacggcgcga cgacgaagat ggacgcgatg
     6541 gtcgactcgc cgagcgtgat gggcgatgtc cccgtcgacg gcaaggatcc aaacaaccca
     6601 ttgacgctcg ccgataccgg cctgtgcctc gatgcgggtt gcacgcagat ctcgacggac
     6661 gtctacgcgt ttcagccgca gttccagctc tggtcggatg ccgcgacgaa gaagcgctgg
     6721 atctatctgc cgcccggcac gcagatcaac accgcgaaca tggacttctg ggagttcccg
     6781 gtcgggacca agctctggaa agaattcacg cgcgacaacg tgcgcgtcga aacgcgcctc
     6841 gtgatgcgca tcggtaacgc gggcatgccg tcggattggt tctacgtgcc gtacgtgtgg
     6901 aatgcgtcgc aggacgcgac gaccgcgcag acagccggcg ttcagaacgc aaacggcacg
     6961 cagcacgacg tgccgagccg cttcgactgc aagagctgcc acgagaacgt caagccgacc
     7021 cgcatcctcg ggttctccgc gatccagctc gactgggata accccgacgc ggcgcagctc
     7081 gatctcaaag agctcgtcgc gaaaaatttg ctgacgaatc cgccggccgc accgtccacg
     7141 gtgggtgcgc catacttccc gctgccgggc acgacatacg agccgcccgc gctcggctat
     7201 ctgcatgcga actgcggcca ctgccacaac ccgacgtcgg gcaactacgg tgcgacgccg
     7261 atggagctgc gcctgaccgt cgcaacgctc ggcgcgctct cgacaacgcc ggcgtacacc
     7321 accgcggtca actgcaacac gaacgtcggc ggtacgacgg cgacgaagat catcgtcgcg
     7381 ggtcagccca gcatgtcggc gatgatcgaa cgcttcgagt cgacgaaccc gaccgtccac
     7441 atgccgcaaa agggcagcga ggtcatggac ccgacgggcg acaccacgct gaagacgtgg
     7501 atcacgaaca tcccctgaga tgcacggagt tcttgtcctc gataagccgc gagacatgag
     7561 ctccgcgtcc gcggtcgagc acgtcaggcg ccggctcggc atcaagcggg caggacacgg
     7621 cggcactctc gatccgatcg cgacgggtgt gctcgcgatc tgcctcggcg aggcgacgaa
     7681 gctcgcgggc tacctgctcg ccgacgacaa agagtacgaa gccgacctcg tgctcggcag
     7741 cgagaccgat acgtacgacc gcaccggtgt cgtgacgtcg acgcgcccgg ccgatcacat
     7801 caccgccgag atgatcgagc gcgtcctcgc cgcgcgcacc ggcgaacagg atcagctgcc
     7861 gccgatctac agcgcgatca agcaggacgg cgtccgcatg tatcaccgcg cgcggcgggg
     7921 cgagacgctc gagacgatcg agcgcacgcc tcgtcgcatc cgcatcgatc gtctcgcgct
     7981 cctcgactac accgcgcccg tcgcgcgcat cgcgatcgcg tgcagcaaag gcacgtacgt
     8041 ccgatcgatc gtcgcggaca tcggcacgga cctcggcgtc ggcgcgcaca tgagcgagct
     8101 ccggcgaacg cgcagcggca ccttcacact cgcgcaggcg cacgacctgg accgcatgga
     8161 caactgccgc atcgtcccgc tcgagcaggc gacgaagctg ccaatcgtca tcgcgtcgga
     8221 cgaccacatc acgaagattc gtaatggcct gcaaatgccg ccggatgtcc tcggcgtagc
     8281 ccccgaaatc ttccagaaat tccagctctt taatgaggcg cgcaagctcg tcgcagtcgc
     8341 ccacgtcgac cgaaaccgcg tcatttatga ccgcgtgttt gcgccttgac cggacgcgcc
     8401 agatacccca atctgcgcct gaccacggaa ttcgttcact tgtggacgcg accgttggcg
     8461 actggagctc gagatgccta cttctatttc gaccgttcgt aagcaagaca ccatcggcaa
     8521 gttccgccag cacggcaccg acaccggttc cccggaagtc caggttgccc tcctcagcga
     8581 gcgcatcgcg cacctgacgg agcacctcaa gaccaaccac aaggaccacc acagccgccg
     8641 tggtctcctc aagatggttg gtcagcgccg cgcgcttctc gactacctga agcgcaagag
     8701 cctcgatcgc tacaagaagc tgatcgaaac gctcggcctg cgccgctaag cgtcctcgct
     8761 ttcgcgcgct ccgtcacgtt aagctcgctc cgtgcagggc acggtgggct acgtcctgat
     8821 cgcggtcggc gtcatcgtcg gttttctcgg caccgcgcgt tcggagcgtg cggcggctcg
     8881 ggatccagac ggtcgcgacc gcatgctcgg tgcgctgcgc aaggcgaagc tcgccgtcgt
     8941 cgtaatcgca gtcgcgatcg cgctgtatct cgcgatgcgg cactacggtc acggtcgcct
     9001 cggcctgcag atctgcaccg cgatcctcgg cgtaaacgcg acggcggttg cggtgctgaa
     9061 gatgcgcgca gccggcgagc atttgagcgg cgacaatcgc gcaacgttca agcgcttcgc
     9121 gatcgccgaa gccatcggct acatcctggt cttcgccggc atctacttgt atctcacgcg
     9181 ttagtcgatg aggcgcatcg cgtcggtagt cgttcgctcg ttgctcctta gctgcgcatg
     9241 caatggctcc tcgaagccga cgcatcctgt cgcgccaccg actgacgcgg cgacacggaa
     9301 cgacgacgac gcgctcgggg caatccgcaa cgacaacatc gagtacgcga cgtacacgcg
     9361 acccgacgat cgcaggaagg tgcctccggg cctgtatcgt caggtgtccg tgaccgctcc
     9421 gcaggaggcg ttcgcgctca ctcgcagcgg cgacatgcga gcgttcgatc tgctcgtgcc
     9481 gatgctcgat gatcccgagc gcaactgggc ggcatttgtc atgctcgcgg cgctcaccgg
     9541 acacgaggcg gccacggtaa tcgcgttcga caacgccgcg ggcttcaagg gctctgccgg
     9601 cgagcgtgat gcgcgcaaga actgggaagc gtggctcgcc gaccatcgcg cgacgctgac
     9661 gtgggacgcc gagcgccacc agtttcgcac cgctcaataa tgatcgccca cgttctgcct
     9721 acttgatgat gacgaacgtc tggtgattgc gcgcgagaag acgcccgctc gcggtccaca
     9781 gctcgcgcgt ctcgaacgag tagccatcgg tgcagaccgg cgacacgccg cgatagagca
     9841 gggcctcgtc gccgacgtcg ctcgcatcgc cggtgacctc gagcgtgaac gcgatggtcg
     9901 ccatcggatg cggctgcttt gcgtgcacga gcgcgaccgg aaaccacgca tcgatcatcg
     9961 ccgcgagata tgcggcatcg cgcttgcggc ccggcgctcg cgcgcgaatc cagccgattg
    10021 ccggacccgc gacgcccgac gtcggaatgc cctcgacgac gcgaaactcg aagtgctgcg
    10081 cgaactctgg gaacggcccg ctggtcggca tcggcttcag cgcttcccac gccggcgcat
    10141 gcggcggcat gagcgaattc cacgcgagcg cggaggtccg cgtcgtcgcc gcgatgacaa
    10201 ccgcatgcgc gcgcgtctcg ccggcctgca cgagcgcgac acgcgcagcg gtgacggact
    10261 tgcccgcgcg caggacctcc acggtgagct ccgcgcggcc cggctcgacc ggcgcaggaa
    10321 tctccgcggt caccgagcga atggttcgcg ctgcatcgcc gatcgcttgc tcggccgcac
    10381 gcatcatcga cgcaacgaca agcccgccgt atgcgcccgc gccttgccgc caaccggccg
    10441 gaacatcgag cacgccgccc tgcggggtag tcgcggtctc gagcgtcatc actggcgagc
    10501 ttagcgtcag gtcgacttcg cggcttcgcg cagcacttct ttgagcgccg tctgcagcgt
    10561 ctctttgccc ggtgcaccgg tgacgcgcgg gacgaccgcg atcgagacgt cgggaatctc
    10621 ggtcgtgccg tcactgacga ttcgatcgct gccgacccag ccgaccgtca cgtccgcggt
    10681 gggcgggccg acgtagccga tcgcgcggat cgtcatccct cggagctgcg cagccgtctt
    10741 gattcggcgg atctgccagt cgccgttttg ctcatccgcc tcgacctcgc cgagcgtcgc
    10801 gagcccgtcg agccacgcgg ttggctcctt ggtcacgacg gtcagcttca cgtccagaaa
    10861 tcccggggtg tcggtcttca taacccggtg atcttactgc agacgggcct ttcggagcgc
    10921 ttggcttttg ctcccgtgtg tgtaataagt gccccggtaa acaactaggt agcgcccagg
    10981 aaatccagac attcggggtc agtttgatcg ttcagtcgag tcgcttcgca cagccgagag
    11041 cgattggatc gagggttcaa gcaggccagg cgaacgaagg ccttccgaag cgcgagtttc
    11101 ggaaaggaca tcgctaatgg gcttcgtacg agagaccgcc gttgtcggcg gcaaagagat
    11161 cacgatcgag acgggcaag
//
LOCUS       DATLBZ010000150         4419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4609651, whole genome shotgun sequence.
ACCESSION   DATLBZ010000150 DATLBZ010000000
VERSION     DATLBZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4609651"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VMZ53_07995"
     CDS             complement(<1..1158)
                     /locus_tag="VMZ53_07995"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HUS28434.1"
                     /translation="MIALLLFILAATVATLCAAADGALLATEGRSAPGAGIEGERAHR
                     ALGVARLLTQVTAGAALAVLLEPNIHPRWLGVPVALLFTALFVTLTESMPRAIGDARA
                     ASLRQRMSGVIHAADSILRPATAVTERLDRTLRRIIVLGADTDESHLTTTAQFRQVVA
                     AEADVSRDDQSLLLGVFSLGDTEVHEIMVPRVDIVGIELDTPWSEMLDRVRSSEHARF
                     PVFVETLDDIAGILYAKDLLPSVIADEHPERGWNAFVRPAVFIPATKTIDSQLRDFQA
                     SRTHIAIVVDEYGGTAGLVTIEDILEEIVGEIQDEYDVEEPAIERENGRRFWVSGKVT
                     LDELSDNLGHDFRHEQVTTVGGLLYEMLGRVPRAGESLAAGPYRIVVERMAG"
     gene            complement(1155..1589)
                     /gene="ybeY"
                     /locus_tag="VMZ53_08000"
     CDS             complement(1155..1589)
                     /gene="ybeY"
                     /locus_tag="VMZ53_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HUS28435.1"
                     /translation="MTLIADVASEGVRVPLSSERVAAIARSVLEAEGVRNALLSITFL
                     DARAMTRLNVRHLRHRGPTDVISFAFDRPARSAVVGDVYICPDVARRNAAAARVGVRE
                     EIARLVVHGTLHVLGYDHPEGDDRVRSPMWRRQERLLARAAR"
     gene            complement(1607..2557)
                     /locus_tag="VMZ53_08005"
     CDS             complement(1607..2557)
                     /locus_tag="VMZ53_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HUS28436.1"
                     /translation="MTETLTTQKISTEGADLLMLAGVNDANLIELSKQCGVQVTLRGD
                     RLSIVGATDSVERALPVAQKMVDAARQRFHLTADDVLRMSDEGERTRTPGDEQRIALP
                     GVRKVIQAKTPGQAEYLRLIAEHDIVIGIGPAGTGKTYLAVAAAVDALVRKRVRRIVL
                     ARPAVEAGESLGFLPGDMQAKVDPYLRPLYDALDEMMPMERIQKALETRVIEIAPLAY
                     MRGRTLGDAFVILDEAQNATGMQMKMFLTRLGVNSKVVITGDKTQIDLPKREESGLMQ
                     IERILAGIEGIAMHYFDEGDIVRHRLVREIVRAYAEDAGD"
     gene            complement(2550..4346)
                     /gene="aspS"
                     /locus_tag="VMZ53_08010"
     CDS             complement(2550..4346)
                     /gene="aspS"
                     /locus_tag="VMZ53_08010"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HUS28437.1"
                     /translation="MAEREIALVKTATSYRTHMCGELRETHVGQQVRLGGWVHRSRDL
                     GGLLFIDLRDRAGLVQLSLDPSKLPAEHSAAASAVGAESVVLVEGEVTSRPSDMRNAD
                     MATGAIEIRVSKFRVVGPARTPAIPVARGRNEKLAAEELRLRHRYLDLRRPEMQERLV
                     LRHRLLQATRRYLSENGFLEIETPMLTRPTPEGARDYLVPSRVHPGEFYALPQSPQIY
                     KQLLMVSGFDRYFQIARCFRDEDLRADRQPEFTQIDIETSFMNIDDLFTVIEGMLVAL
                     WKEAGRDVKGPFQRMAHAEAMERFGTDRPDMRYDLELFDASDAFRGADFGITKSALAA
                     GGRVRGLRIPGGATMSRKQVDELEAIAKGGGASGLLRVKRTGDALDGPVAKFLPANGA
                     ELLGLADGDLCLMVAGPDRVSSPSLDRVRQEVASRLNLSRESESRFLWITDFPLFAHG
                     EGDMLVPEHHPFTAVHPDDVGLLDSNPGKARALAYDVVYNGLELASGSMRINDPAMQE
                     RVFALLGIDEADAMKRFGFLLEGLRAGAPPHGGIAFGFDRISMILANGESLRDVIAFP
                     KTTAARSLFEDAPTTVPRADLDVLHIAPVSHD"
BASE COUNT          780 a         1504 c         1399 g          736 t
ORIGIN      
        1 gccggccata cgctcgacca cgatgcggta cggacctgcc gcgagcgact cgcccgcccg
       61 cggcactcgg ccaagcattt cgtagagcag tccgccaacc gtcgtcacct gctcgtgacg
      121 gaaatcgtgg ccgaggttgt cggacaattc atcgagcgtc accttccccg acacccagaa
      181 gcgccgcccg ttctcacgct cgatcgcggg ctcctcgacg tcgtactcgt cctgaatctc
      241 gccgacgatc tcctcgagaa tgtcctcgat ggtgaccagc cccgcggtgc cgccgtattc
      301 gtcgacgaca atcgcgatgt gcgtgcggct cgcctggaag tcgcggagct gcgagtcgat
      361 cgtctttgtg gccggaatga acaccgccgg gcgaacgaag gcgttccagc cacgctcggg
      421 atgttcgtcg gcgatcaccg acggcagcag atcctttgcg tacagaattc ctgcgatgtc
      481 gtccagtgtc tcgacaaata ccgggaaccg cgcatgctcg gagcttcgca ctcggtcgag
      541 catctccgac cagggcgtat cgagctcgat gcccacgatg tctacgcgcg gcaccatgat
      601 ctcgtgcacc tcggtgtcgc ccagactgaa cacgccgagc agcagcgact gatcgtcgcg
      661 cgacacgtcc gcctcggcgg cgacaacctg ccggaactgc gctgtcgtgg tcagatgcga
      721 ctcgtcggta tccgcgccga ggacgatgat gcgccggagt gtgcgatcga gtcgctccgt
      781 cactgcggtt gccggacgca ggatcgaatc ggccgcgtga atcacacccg acatccgctg
      841 gcgcagcgac gccgcccgcg cgtcaccgat cgctcgtggc atgctctcgg tgagcgtgac
      901 gaagagcgcc gtgaacagca gcgcgacggg cacacccagc cagcgcggat gaatgttcgg
      961 ctcgagcagc accgcgagcg cggcaccggc ggtcacctgg gtcagcagcc gtgccacgcc
     1021 aagtgcccgg tgggcacgct cgccttcgat accggcgccg ggcgcgctgc gcccctcggt
     1081 cgcgagcagc gcgccgtcgg cggctgcgca cagcgtggca accgtcgccg ccagaatgaa
     1141 gagcaggagc gcgatcatcg cgccgcccgc gcgagcagac gttcctggcg gcgccacatc
     1201 ggtgagcgca cccggtcgtc accctcggga tgatcgtagc caagcacgtg cagggtgccg
     1261 tggacgacga ggcgcgcaat ctcctcgcgc actccgacgc gtgccgccgc cgcatttcgt
     1321 cgcgcgacat cgggacagat gtacacgtcg ccgacgaccg ccgaacgcgc cggccgatcg
     1381 aaggcgaagg agatcacgtc ggtgggaccg cggtggcgca ggtgacgcac gttgagccgc
     1441 gtcatcgcgc gcgcgtcgag aaaggtgatc gacaggagcg cattgcggac cccttccgcc
     1501 tcgagcaccg agcgggcgat ggccgcaacg cgctcgctgg acagcggaac gcgcactcct
     1561 tcacttgcca cgtcggcaat gagcgtcatg gcagacgacc cgaaggtcag tcgcccgcgt
     1621 cttcggcgta cgcgcgcacg atctcgcgta cgaggcggtg ccggacgatg tcgccttcgt
     1681 cgaaatagtg catggcaatg ccctcgatgc cggcgagaat tcgctcgatc tgcatcaacc
     1741 cggattcttc ccgcttcgga agatcgatct gcgtcttgtc accggtgatg acgaccttgg
     1801 agttcacacc aagccgcgtg agaaacatct tcatctgcat gccggtcgcg ttctgcgcct
     1861 cgtccagaat cacgaatgcg tcgccgagtg tccggccacg catgtaggcg agcggcgcaa
     1921 tctcgatgac ccgcgtctcg agtgctttct gaatcctttc catcggcatc atttcgtcga
     1981 gcgcgtcata caacgggcgg agatacggat ccacctttgc ctgcatgtca ccagggagaa
     2041 atccgagact ctcgccggct tcgacggcag gccgcgcaag cacgatgcga cgcacacgct
     2101 tgcgaacgag agcgtcgaca gccgccgcca cggcgagata cgtcttcccc gttcccgcgg
     2161 gcccgatccc gatcacgatg tcgtgctctg cgatgagacg caggtattcg gcctggccgg
     2221 gcgtcttggc ctggatgact ttgcgtacgc cgggaagcgc gatgcgctgt tcgtcgccgg
     2281 gcgtgcgcgt gcgctcgcct tcgtcgctca tccgcaggac gtcatccgcc gtcaaatgga
     2341 atcgctgacg cgcggcgtcg accatcttct gcgccaccgg gagcgcacgc tcgacagaat
     2401 cggtggcacc gacgatcgac agccggtcgc cgcgaagcgt cacctggacg ccgcactgct
     2461 tcgacagctc gatgagattg gcgtcgttga cgccggcgag catcagcagg tcggcgccct
     2521 cggtggaaat tttctgcgtg gtcagggttt cagtcatgtg acaccggtgc gatgtgcagc
     2581 acgtcgaggt ccgctcgcgg gaccgtggtc ggcgcgtcct cgaacaatga gcgcgcagcc
     2641 gttgttttcg ggaaagcaat cacgtcacgg agcgactctc cgttcgcgag aatcatcgag
     2701 atccggtcga agccgaaggc gatccctcca tgcggcggcg caccggcacg aagtccttca
     2761 agcaggaagc caaagcgctt catcgcgtca gcttcgtcga tgccgagcaa cgcgaagacg
     2821 cgctcctgca tcgcgggatc gttgatacgc atgctgccgc tggccagctc gaggccgttg
     2881 tagacgacgt cgtacgcgag cgcgcgggcc tttccaggat tgctgtcgag aagcccgacg
     2941 tcatccgggt gcaccgccgt gaaggggtgg tgctcgggaa cgagcatgtc gccctctccg
     3001 tgcgcgaaga gcggaaaatc ggtgatccag agaaatcgtg actcgctctc gcgcgacagg
     3061 ttgagccgac tggccacttc ctgacgcacg cgatcgagcg acggactgga gacacgatcc
     3121 ggccccgcga ccatcagaca gagatcaccg tccgctagac caagcagctc ggcaccattc
     3181 gccggcagga acttcgcgac cgggccgtcg agcgcgtcgc cggtgcgctt gacgcgcagc
     3241 agccccgacg cgccgcctcc cttcgcgatc gcctccagct cgtccacctg cttgcgcgac
     3301 atagtcgcgc cgcccggaat ccgcagcccg cgcactcgac cgcccgctgc cagcgcgctc
     3361 tttgtaatgc cgaaatcggc gccacgaaac gcatcgctcg cgtcgaacag ctcgagatcg
     3421 taacgcatgt cggggcgatc ggtgccgaac cgctccatcg cctcggcatg cgccattcgc
     3481 tgaaacggcc ccttcacgtc gcgtcccgct tccttccaca gcgcgacgag catcccttcg
     3541 atcaccgtga agagatcgtc gatgttcatg aaggaggttt cgatgtcgat ctgcgtgaac
     3601 tcgggctgac gatcggcacg cagatcctcg tcgcggaaac aacgcgcgat ctggaagtag
     3661 cgatcgaagc cggacaccat cagcagctgc ttgtagatct gcggcgactg cggcagcgcg
     3721 tagaactcac cgggatgcac gcggctcggc acgaggtaat cgcgcgcgcc ttcgggcgtc
     3781 gggcgcgtga gcatcggcgt ctcgatctcg agaaagccgt tctcgctcag atagcgacgc
     3841 gtcgcctgca gcagccggtg gcgcagcacc agccgttcct gcatctcggg gcgccgcagg
     3901 tcgagatagc gatgacgaag cctcagctcc tcggcggcaa gcttctcgtt tcggccgcgc
     3961 gcaaccggaa tcgccggcgt gcgcgccggg ccaacgacac ggaatttcga cacgcgaatc
     4021 tcgattgcgc cggtcgccat gtcggcgttg cgcatgtcgg acggacggct cgtgacttcg
     4081 ccctcgacga gcacgacact ctccgcgccg acggcactcg ctgccgctga atgctccgcg
     4141 gggagctttg acgggtcgag tgacagctgc accagcccgg cgcgatcgcg caggtcgatg
     4201 aacagcaggc ccccgaggtc acgactgcga tgcacccagc cgccaagacg cacctgctgt
     4261 ccgacgtggg tctcacggag ctcaccgcac atgtgcgtcc gatagctcgt cgccgttttc
     4321 acgagagcga tctcgcgctc agccatcgct tgccgaagag agtgattgcg atcgcgccca
     4381 gagcgccgcc gatcgtgtcg gccatccagt cgcgcacgt
//
LOCUS       DATLBZ010000151        12205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3869579, whole genome shotgun sequence.
ACCESSION   DATLBZ010000151 DATLBZ010000000
VERSION     DATLBZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3869579"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VMZ53_08015"
     CDS             complement(<1..718)
                     /locus_tag="VMZ53_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HUS28438.1"
                     /translation="MMRRALLCLLAGCGDDSGAQPDAPSTTTEGWTQAGDVPHGAIQE
                     TAAVGVHGKIYVLGGFDSDDGIVALVQVFDASAGAWTNDATPLPRALHHINAVTDGDT
                     IFVTGALSGVNFTPVGDTYSFTPGGTWQTRAAMPVGRERGAAVTGIIDGKIYVAGGFR
                     GGRAAALVDVYDPVADTWVARADLPVARDHACGGVLDGKLVVAGGRDGSPSGPVPDVW
                     AYDPATNTWTQRAPMPTGRGG"
     gene            complement(715..1518)
                     /locus_tag="VMZ53_08020"
     CDS             complement(715..1518)
                     /locus_tag="VMZ53_08020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase, TIGR04325 family"
                     /protein_id="HUS28439.1"
                     /translation="MGAKKVLQQIAPPFVWTVAHRVRQGLQGRQAAYDTYDEALAAAG
                     GPGYDVEAVAEGTVAQFAREAETVRGRFPFADVRTIRQLAAMWRVRDALGGKPLRVLD
                     VGGQVGVHFQRLRVHLPENAIAAWDVVETGSLARLAKAKCTEPGLRFFEGLESLPAGD
                     YDVAFSSGTLQAVPDPWAFFESVGSRAKWLLLDRIPFGAFERDRLMVLRNALETYRVA
                     IPIWFFAETTWLERFAASGWKPVLRWRAPEETITFDGVPVEYSGLLLQR"
     gene            1596..2765
                     /locus_tag="VMZ53_08025"
     CDS             1596..2765
                     /locus_tag="VMZ53_08025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28440.1"
                     /translation="MPTRFGDYVIREPLGNGGMASVHLADWKPPKGVARRVALKRLYP
                     HIAENPELVKMFIDEARLSRYLNHPNVAQVYEFGRIQGTYFIAFEFVEGPTVYQLAKH
                     CYANVGYIPIPIILEIAIQLCDALIHAHNLCDADGLPLGIVHRDISPQNLIVSTVGFV
                     KLIDFGLAKAKESSAESHSGVIKGKVHFVAPEYLDGQLDVRCDLWAVGVLLHELLSGR
                     PLFDAPDHFKMVDLVRNMAIPPPSRFRAEVPTGIDDIVFKALERKPAKRWQNAASLRA
                     ALVKEAKELSPVTKSQLVTWVEWAFAQKYKTREDSGLAALSQIISVPFELVEEPTNPP
                     QLPATSAAVEERRRESLAVMPVGEAMLRANRGSRWPWIVGALVLAALVVLALVLL"
     gene            2854..3672
                     /locus_tag="VMZ53_08030"
     CDS             2854..3672
                     /locus_tag="VMZ53_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28441.1"
                     /translation="MGECSTRRRVALVAAVALAGCAKANSGGTPDAGPGESFFDAAPD
                     ATCGNMCDHDHDGVFDPDDKCANTPMGQVVNGVGCADSQLTPTLVPFPPFGLTWTPTG
                     DLGRAAKLTWTYVGIDRKDLFHIYWLVCDDPATPCGLSLDGPIDVAAEQWQYSATGSD
                     LPNGKLVFTNATHISLANGTSPALDGRLTITIVDATDVAIPFATVAGLSVTPLAAAYG
                     AEIKGAGFKVVVTAEVLDTTTATYKPYLDYYDAAPTPATGASTATSFGGSFYAK"
     gene            3828..5039
                     /locus_tag="VMZ53_08035"
     CDS             3828..5039
                     /locus_tag="VMZ53_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HUS28442.1"
                     /translation="MPGRLSLSLTNALLPIHSVETLDEAMGPVVYDERLVFWETRLAA
                     EYTLTRSLAVGAAFPYRVVDVDVANSDPATGDPITVTNVHARNETLTGVGDPSVSAHL
                     ATDAAGLHVHARIGTSLPLGRTEEDPHLLGSLGQEHEHIQLGTGTFIPFVSVEAQRGF
                     GKVVGAAWALAHLSLYDNDHGYRAGDRYSGGLTAASGLGLRRWTFGVALEGHGETGET
                     WQGVVHRDEGNAGRFDLMAGANVAWRPVDHLAVILDAKLPVYTYVDGPQLDYGLIVGI
                     GIAATFDLKPRASWHGLDEKVVADAGVAAELVPVPGKITVFDLWAEWCAPCRELDDRL
                     AAIARAHPDKLAVRKLDVVDTDSAAWERYMAPKQFDLPHVKVYGADGALLFERSAPPA
                     ELARAVEEVLR"
     gene            complement(5040..5471)
                     /locus_tag="VMZ53_08040"
     CDS             complement(5040..5471)
                     /locus_tag="VMZ53_08040"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HUS28443.1"
                     /translation="MRLYALLFMCACGSDPTPLIEKGYASSFTEVRNCRSSSDHDLHR
                     IRVVADGASLDPYLTRATPFPLGATIVKEEYDFADTACAGDILEWTVMQRIDGDWRWQ
                     RLEPGNGADLRVKSDDDARCINCHATCGAPPDGYEGTCTVP"
     gene            complement(5475..6731)
                     /locus_tag="VMZ53_08045"
     CDS             complement(5475..6731)
                     /locus_tag="VMZ53_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28444.1"
                     /translation="MKAKLACLVLLVAACGEDAPSHCDPNVVGTICTIAGAGQSGYSG
                     DDGDALEARMSLPQDTLKIGDALYILDWNNHRIRRLDPDGVIRHVAGRGELGGTLDDP
                     ANSDFNHPTGLVMRPGSNQLVVAAWHNSKLRTVDLTTGTIADHCGDGRRAYFGDNGPA
                     LMSSLDLPASVAYAPNGDLVIMDQANQVMRKIDTSGTITRLAGRCVVDAPAPLGGGPC
                     ADGVEPTQCPPPSGKWTCGAMGTCAAPCTPGYGGDDGPAINLRMSQPFGQSADPAGRI
                     AFDSSGNLFFADTVNGIVRKIDPAGNVTRVAGTAPVGGVAQTGYAGDGGPARDAKLFN
                     PVDLAFDTDGTLYVSDVYNHCIRAISPSQTITTVAGMCGQSGSSGDGQPATTALLKRP
                     YGIEIADGVLYIADTGNNSIRSVKLR"
     gene            complement(6728..7888)
                     /locus_tag="VMZ53_08050"
     CDS             complement(6728..7888)
                     /locus_tag="VMZ53_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28445.1"
                     /translation="MRFVAVVLLAAAAFGCGDNDGHACADESGIACNFIGKPGVYGFN
                     GDNHKRLDTELYWTMDLSFAADGTIWFIDWNNHLVRRVMTDDTVTSVVGWIDPIFPGD
                     GVPGTAERSADGVVGTDVQLNHPTDLAQEPDGKVLVMAWHNHKLREVDPATGHVHIVA
                     GGGAGYSGDGATIATALFKQPKALELDAAGNMFILDQQNFRIRKIERATGAMSLVAGI
                     GMQGSDGDGGPAVDAKLNFEAGSNPEPSGGMVFANGKLYVSDTLSSRVRAIDMTTGII
                     TTVVGTGTKGYAGDGGPANAAQLAFPRDLEIGPEGDLYIADTDNSVIRAVNLTTNVIR
                     TVAGTGELGLDATDGLPATETKLARPFGIEFDKAGNLYICDTINSRILKVAR"
     gene            complement(7921..8295)
                     /locus_tag="VMZ53_08055"
     CDS             complement(7921..8295)
                     /locus_tag="VMZ53_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28446.1"
                     /translation="MTKLTLMFIVTLGVVGCGGKKEDKPAATGGGAPAAKAGAKGDCL
                     VGTYEYLDGGKAKQMVFNADKTGHDDKADGKTPDKFNWSLKDEKTVHIVYPAEGDNMG
                     GEFDMPFDCASGTFYTLYHKKP"
     gene            8327..9358
                     /locus_tag="VMZ53_08060"
     CDS             8327..9358
                     /locus_tag="VMZ53_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28447.1"
                     /translation="MRCRLAWMLVSIHALAGCGRLAFDANADADANADAPVCTANIDL
                     DRDSTNCGACGHDCLGGVCEQGACQPIALDTSSNELRSIVLDDTYLYFAGVASIGRIP
                     KSGGAVQTVATFSGGAFRMTEHAGYLYFTIPDALQIARTPMTSQAAPAEIIDTNISFL
                     SGIESDGTYVYWATTESTARVRRTEIATGIRSDVTTTDGGPIHGLRLVGSTLYFLHES
                     VGLESIHADGSSGSIVAPAGTFEAEIDGGVAFMTSLTRDAIVRVSLADGAVTPLAPAD
                     GPWGLAIDDTNVYVANEVGGWIGRVARTGGTQQQVVLASVTDAVGVAVDDKAIYYTAR
                     YGGIYKLAK"
     gene            complement(9376..11124)
                     /locus_tag="VMZ53_08065"
     CDS             complement(9376..11124)
                     /locus_tag="VMZ53_08065"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HUS28448.1"
                     /translation="MRLVFAVTLLMACGPSVRDSDGDGGNGGDALPMGTQCSSDLHEV
                     LDDAGNVVGTCPPDQGCAAGACVPACQAAAASQGSVGCDFVVATPSFYSSISPPCFAV
                     FLANNWPSDATVTITRGTQTFDATMFGRVPNGSNDAATWPTIAATGIASSNVGVLFLS
                     HDPAAANATIPMTCPVPPAVSGTGGSAVWTGGDSATGIGTAFHITTIVPVSAYDIMPY
                     GAATSFLPSAELLLPTSAWGTNYVTGGPAPTSGAGWGQLVAAADNTTVMISPTRALPA
                     GSGVAAAPASTTTTYTLQAGQYIQWQATGEMGGSVIQSDKPVSFTGGTTYLCQSTTTS
                     SGGGCDSGHQLVPPVSALGYQYVIPPHATRRANLQPESIVYRLVGAADGTTLTYSSAV
                     TGAPATLNRGQVLQFQATGAFTITSQDDMHPFYVGQYMAGCMVSGGSRPGSVADQITG
                     TQCLGDEEYVNILPPAQWLSSYVFFTDPTYTTTNLVVTRKAQGGSFQDVTVDCLGVIS
                     GWQPVPGSSEYQITNVDLQRVTPVGTCTNGRHTATSGGAFSIMVWGLATFASYAYPAG
                     GNVGKINPVVVILRPQ"
     gene            complement(11302..11766)
                     /locus_tag="VMZ53_08070"
     CDS             complement(11302..11766)
                     /locus_tag="VMZ53_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUS28449.1"
                     /translation="MSNTKTTQVFRASEGKKYQLGRLAISFKRTDGEDEGSYSLFETA
                     EPVGAGAGVHRHPGFQETFIVIEGTFTFEVDAVTHDVGPCDVIVIPRGAWHGFRCTSP
                     QPGRLLTISSPARVFEAGVAEICAANVDTGSPDGGPAHDMRAIAARHGLEFR"
     gene            complement(11813..12007)
                     /locus_tag="VMZ53_08075"
     CDS             complement(11813..12007)
                     /locus_tag="VMZ53_08075"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvA C-terminal domain-containing protein"
                     /protein_id="HUS28450.1"
                     /translation="MGRASGAPPHLEMDDPATLAQAALRQLGFKAALAARAVEEACAH
                     VGTDVDLGALIREALRHCGE"
     gene            12021..>12205
                     /locus_tag="VMZ53_08080"
     CDS             12021..>12205
                     /locus_tag="VMZ53_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28451.1"
                     /translation="MTDRTTSDRATSNRATSRARVIALFSRVGSRDSNPANTQEAEQA
                     TVCPLVSCVPSLLAAVA"
BASE COUNT         2090 a         4169 c         3858 g         2088 t
ORIGIN      
        1 taccgccgcg gcccgtcggc atcggagcgc gttgcgtcca cgtattcgtc gccggatcgt
       61 acgcccacac atcaggcacg ggcccgctcg gcgatccatc gcggccgcca gcgacgacga
      121 gcttgccatc gagcacaccg ccgcatgcat gatcgcgcgc aaccggcaga tccgcgcgcg
      181 ccacccacgt atccgcgacc ggatcataaa cgtcgacgag cgccgccgcg cgaccgccgc
      241 gaaagccacc cgcgacgtag atcttgccgt cgatgattcc cgtcaccgcc gctcctcgct
      301 cgcgacccac cggcatcgcc gcgcgcgtct gccacgtacc gcccggcgtg aagctgtacg
      361 tatcgccgac cggcgtgaag ttgacgccgc tgagcgcgcc cgtgacgaag atagtgtcgc
      421 cgtccgtcac ggcgttgatg tgatgaagcg cgcgaggcag tggtgtcgca tcgttcgtcc
      481 acgcgccagc gctggcgtcg aagacctgga caagcgcgac gatgccatcg tccgaatcaa
      541 agcccccgag cacgtaaatc ttgccgtgga cgccgaccgc cgcggtctcc tgaatcgcgc
      601 cgtgcggaac atcgcccgct tgcgtccacc cttcggtcgt cgtcgacggc gcatcgggtt
      661 gcgcaccact gtcgtcaccg cagccggcga gcaggcagag cagggcgcga cgcatcatcg
      721 ctgcagcaac aatcccgagt actcgaccgg cacgccgtcg aatgtgatcg tctcctcggg
      781 cgcgcgccag cgcagcacag gcttccatcc gctcgctgca aagcgctcga gccacgtggt
      841 ctccgcaaag aaccagattg gaattgcgac gcggtacgtc tcgagcgcgt tgcgcagcac
      901 catcaggcga tcgcgttcga aggcgccgaa tggaatgcga tcgagcagca accacttcgc
      961 gcgcgagccg acgctctcga agaatgccca cggatcgggc acggcttgca gtgttcccga
     1021 cgagaacgcg acgtcgtagt cgcccgccgg aagtgactcc aggccctcga agaaacggag
     1081 tccgggttcc gtacacttcg ctttcgcgag gcgcgccagc gacccagtct cgacgacgtc
     1141 ccacgcagcg atcgcgttct cgggaaggtg gacacgaagg cgttgaaagt gcacgcccac
     1201 ttggccgccg acgtcgagca cgcgcagtgg cttgccgccg agtgcatcgc gcacgcgcca
     1261 catcgcggcc agctgccgga tcgtgcggac gtccgcgaac ggaaaccgcc cgcgcaccgt
     1321 ctccgcctcg cgtgcaaact gcgcgacggt gccttcagcg accgcctcga cgtcgtagcc
     1381 ggggccaccc gcagcggcga gggcctcgtc ataggtgtcg tacgcggcct gccgtccctg
     1441 cagtccttgg cgcacccgat gcgcgacggt ccacacgaac ggaggcgcga tctgctgcag
     1501 cactttcttg gctcccatga ggttcgcgag cttacttggg gatcgcgcct gaacctcgcg
     1561 actcgggacc gttttcgggt gaacatggag acgccatgcc gacgcgcttc ggcgactacg
     1621 tgatccgcga gccgctcggc aacggtggca tggcatcggt ccatctcgcc gattggaagc
     1681 cgccgaaggg cgtcgcgcgt cgggtcgcgc tgaagcggct gtatccgcac atcgcggaga
     1741 accccgagct cgtcaaaatg ttcatcgacg aagctcgcct ctctcggtac ctgaatcatc
     1801 cgaacgtcgc gcaggtgtac gagttcggcc gcatccaggg cacgtacttc atcgcgttcg
     1861 aattcgtcga gggcccgacg gtctaccagc tcgcgaagca ctgctacgca aacgtcggct
     1921 acatcccgat tccgatcatt ctcgagatcg cgatccagct ctgcgatgcg ctgattcacg
     1981 cgcacaacct gtgcgacgcc gatgggctgc cgctcggcat cgtccatcgc gatatctcgc
     2041 cgcaaaatct catcgtctcg acggtgggct tcgtgaagct cattgatttt gggttggcga
     2101 aagcgaagga gtcgtccgcc gagagtcact ccggcgtcat caaggggaaa gtgcacttcg
     2161 ttgcgcccga gtatctcgac ggccagctcg acgtgcgctg cgacctgtgg gcggtcggcg
     2221 tgctgctgca cgagctgctc agtggccggc cgctgttcga tgcgcccgat catttcaaga
     2281 tggtcgacct cgttcgcaac atggcgatcc caccgccgtc gcggtttcgc gccgaggtgc
     2341 cgacgggcat cgatgacatc gtgttcaaag cgctcgagcg caagccggcg aagcgctggc
     2401 aaaacgctgc gagcctgcgc gccgcgctcg tcaaagaggc aaaggagctg tcgcccgtga
     2461 cgaagtcgca gctcgtcacg tgggtcgagt gggcgttcgc gcagaagtac aagacgcggg
     2521 aagacagcgg cctcgccgcg ctttcgcaga tcatcagcgt gccgttcgag ctggtcgagg
     2581 aaccgacgaa cccgccgcag ttgccagcga cgtcggctgc cgtcgaagag cgtcgtcgcg
     2641 agagccttgc cgtcatgccg gtcggcgaag cgatgctgcg cgccaaccga ggcagtcgct
     2701 ggccttggat cgtcggcgcg ctcgtgctcg cggcactcgt cgtgcttgcg ctggtactgc
     2761 tctaggtgtg acggaacgcg cggaagccgc agggttttcg gaccctcgaa cgcatgattg
     2821 acggtggcgg agcgtcatga cagggttcga tcggtggggg agtgctcgac tcgtagacgc
     2881 gttgcgctgg tcgctgctgt tgcgctagcc ggctgcgcga aagcgaactc cggtggcacg
     2941 cccgacgctg gccccggcga gtccttcttc gacgctgcac ccgacgcgac gtgcggcaac
     3001 atgtgcgacc acgatcacga tggcgtgttc gaccccgacg acaagtgcgc gaacacgccg
     3061 atggggcagg tcgtgaacgg tgtcggttgc gcagactcgc agctcacgcc aacgctcgtc
     3121 ccgttcccgc cgttcggcct gacgtggacg ccaacaggcg atctcggtcg cgccgcaaag
     3181 ctgacgtgga cgtacgtcgg tatcgatcgc aaagacctgt tccacattta ttggctcgtc
     3241 tgcgatgacc cggcgacgcc gtgcggtctc tcgctcgatg gcccgatcga tgtcgcggcc
     3301 gaacaatggc agtactccgc gactggctcg gatctgccga acggcaagct cgtcttcacg
     3361 aacgcgacgc acatctcgct cgcgaacggc acgagcccag cgctcgatgg ccgcctcacg
     3421 atcacgatcg tcgatgccac ggacgtcgcg atcccattcg caaccgttgc cggcttgagc
     3481 gttacgccgc tggcggccgc gtacggcgcg gagatcaaag gcgcgggctt caaggtcgtc
     3541 gtgaccgccg aggtgctcga cacgacgacg gcgacgtaca agccgtatct cgactactac
     3601 gacgcagcgc cgacgccggc gacgggcgcg tcgaccgcga cgtcgtttgg cggctccttc
     3661 tacgcgaagt aggagcacgc gcacgttgct gtgctagcgt cgaatcgctg cgtacttctt
     3721 ggtttctcgt cgccgcttcg ctcgccgttc cgcagctcgc acatgcgggg cgcgcgacgt
     3781 gcagcaatcc tgggttgccc gtcggctccg ctgcatcgag cgagctcatg ccggggcggc
     3841 tcagcctcag cctgacgaat gcactgctgc cgattcacag cgtcgagaca ctcgacgagg
     3901 cgatgggacc ggtcgtgtat gacgagcggc ttgtcttctg ggagacacgg ctcgccgccg
     3961 agtacacgct gacacgctcg ctcgcggtcg gcgcggcgtt tccatatcgc gtcgtcgatg
     4021 tcgatgtcgc gaattcggat ccagcgacgg gcgatccgat cacggtcacg aacgtccacg
     4081 cgcgcaatga aacgctgacg ggcgttggcg atccgtcggt gagcgcgcat ctcgcgaccg
     4141 acgcagccgg cctgcacgtg catgcgcgaa tcgggacgag cttgccgctc gggcgcaccg
     4201 aagaagatcc gcacttgctc ggcagcctcg gccaggagca cgagcacatc cagctcggca
     4261 ccggcacgtt catcccgttc gtctccgtcg aagcgcagcg tggcttcggc aaggtcgtcg
     4321 gcgctgcgtg ggcgctcgcg catctctcgc tctacgacaa cgaccacggc tatcgcgcgg
     4381 gcgatcgata ttccggaggt ctcaccgcgg catcggggct cgggcttcgt cgctggacat
     4441 tcggcgtcgc gctcgaaggg cacggcgaga ccggcgagac gtggcaaggc gtcgtccacc
     4501 gcgatgaagg caacgcgggc cgcttcgatc tgatggccgg tgccaacgtc gcgtggcggc
     4561 cggtcgatca ccttgccgtg atcctcgacg cgaagctgcc cgtgtacacg tacgtcgacg
     4621 ggccgcagct cgactacggc ctcatcgtcg gcatcggcat cgcggcgacg tttgatctga
     4681 agccgcgcgc ttcgtggcac gggctcgacg aaaaggtcgt tgcagatgcg ggcgtcgcag
     4741 cggagctcgt gccggtgccg ggcaagatca cggtcttcga tctgtgggcc gagtggtgtg
     4801 cgccgtgccg cgagctcgat gatcgcctcg ccgctatcgc tcgcgcgcat cccgacaagc
     4861 tcgcggtgcg caagctcgat gtcgtcgaca cggatagtgc cgcatgggag cgctacatgg
     4921 cgccgaagca gttcgatctg ccgcacgtca aagtctacgg cgcagacggc gcgcttctgt
     4981 tcgagcgcag tgcgccgccg gccgagctcg cgcgcgcggt cgaagaagta ctgcgctgac
     5041 tacggaacag tgcaggtgcc ttcgtagccg tcgggcggag cgccgcacgt cgcatggcag
     5101 ttgatgcagc gcgcgtcgtc gtcggacttg acgcgaaggt cagcaccatt gccgggctcg
     5161 agccgttgcc agcgccaatc gccgtcgatg cgctgcatga ccgtccactc gaggatgtcg
     5221 cctgcgcacg cggtatccgc gaagtcgtat tcctctttga cgatcgtcgc gccgagcggg
     5281 aagggcgtcg cgcgcgtcag gtacggatcg agtgacgctc catccgcgac aacgcgaatg
     5341 cgatggagat cgtgatcaga gctcgagcgg caattgcgaa cctccgtaaa cgagctcgca
     5401 tagcccttct cgatgagcgg cgtcggatcg gacccgcatg cgcacatgaa caacagtgcg
     5461 tacaacctca tgagctagcg cagcttgacc gagcggatcg agttgttgcc ggtatcggcg
     5521 atgtagagca cgccgtccgc gatctcgatg ccatacggcc gcttgagcag cgccgtcgtc
     5581 gcgggttggc catcaccgct gctgcccgac tgcccgcaca tcccagcaac cgtcgtgatc
     5641 gtctggctcg gcgagatcgc gcggatgcag tggttgtaca cgtcgctgac atacagcgtg
     5701 ccatcggtat cgaacgcgag atcgacggga ttgaagagct tcgcgtcgcg cgcaggtccg
     5761 ccgtcgcccg catagcccgt ctgcgccacg ccgccgacag gagccgttcc cgcaacgcgc
     5821 gtgacattcc cagccggatc gatcttgcgg acgattccat tcacggtgtc ggcgaagaac
     5881 aagttgccgc tgctatcgaa cgcgatgcgc ccggcgggat ccgccgactg cccaaacggc
     5941 tgtgacatgc gcaggttgat cgccggtccg tcgtcgccgc catacccagg cgtgcagggc
     6001 gccgcgcacg tgcccatcgc gccgcatgtc cacttccccg acggaggcgg gcactgcgtt
     6061 ggctcgacgc catcagcgca aggcccaccg ccgagcggcg ccggtgcatc gaccacgcag
     6121 cgacccgcga ggcgcgtgat cgtgccgctc gtgtcgatct tgcgcatgac ctgattcgct
     6181 tgatccatga tcacgagatc gccgttcggc gcatacgcaa cgctcgccgg cagatccagc
     6241 gacgacatca gcgcaggtcc attgtcgccg aaatacgcgc gccgcccgtc gccacagtga
     6301 tcggcgatcg tccccgtcgt caggtcgacg gtcctcagct tgctgttgtg ccacgcggca
     6361 accacgagct gattgctgcc ggggcgcatg acgaggccgg tcggatgatt gaagtcggag
     6421 ttcgccggat catcgagcgt gccgccgagc tcgccgcgtc cagccacatg ccggatcaca
     6481 ccgtcgggat cgagccgccg gatccgatgg ttgttccagt cgaggatgta gagcgcgtcg
     6541 ccgatcttga gcgtgtcctg cggaagcgac atgcgtgcct cgagtgcatc gccgtcgtcg
     6601 cccgaatatc cgctctgccc cgcgccagcg atcgtgcaga tcgtgccgac gacgttcgga
     6661 tcgcaatggg acggcgcgtc ctcgccacac gccgcgacca agagcaccaa acaagcgagc
     6721 ttcgccttca tcgcgccacc ttcaagatgc ggctgttgat cgtgtcgcag atgtagaggt
     6781 tgccggcctt gtcgaactcg atgccgaacg gacgcgcgag cttcgtctcg gttgcaggca
     6841 acccatcggt cgcatcgagc ccgagctcgc ccgtaccggc gacggtgcgg atgacgttcg
     6901 tcgtcaggtt cacagcgcga atgacgctgt tgtcggtgtc cgcgatatag aggtcgccct
     6961 cgggcccgat ctcgagatca cgcgggaacg cgagctgcgc cgcgttcgcc ggtccgccgt
     7021 cgcccgcata ccccttcgtt cccgtcccaa cgaccgtcgt gatgatgcca gtcgtcatat
     7081 cgatcgcgcg cacgcggctc gacagcgtgt ccgacacata gagcttgccg ttcgcaaaga
     7141 ccattccgcc cgacggctcc gggttcgagc ccgcctcgaa gttcagcttc gcatcgacgg
     7201 ccggcccacc atcgccatcg ctaccctgca tgccgatgcc cgcaacgagc gacatcgcgc
     7261 cggtcgcgcg ctcgatcttc cgaatccgaa agttctgctg atcgaggatg aacatgttgc
     7321 ccgccgcgtc gagctcgagc gccttcggct gcttgaacaa cgcggtcgcg atcgtcgcgc
     7381 catcgccgct gtatcccgca ccgccaccgg caacgatgtg cacatgcccc gtcgccggat
     7441 cgacctcgcg cagcttgtgg ttgtgccacg ccatcacgag caccttgccg tccggctcct
     7501 gcgccaaatc agtcggatga ttcagctgca cgtcggtccc gacgacgcca tccgccgatc
     7561 gctccgcggt gcctggcacg ccgtcgcccg gaaagatcgg atcgatccag ccgacaacgc
     7621 tcgtcacggt gtcatccgtc atcacgcgcc gaacgagatg attattccag tcgataaacc
     7681 agatcgtgcc atccgcggcg aacgacaggt ccatcgtcca gtagagctcg gtatcgaggc
     7741 gcttgtggtt gtcgccgttg aagccgtaca cgcctggctt gccgatgaag ttgcaggcaa
     7801 tgcccgactc atcggcacac gcgtgcccgt cattatcgcc gcagccaaac gcagccgcag
     7861 cgagcagcac caccgctaca aatcgcatcg gcgcatggtg acattggcct cgccggttcg
     7921 ctacggcttc ttgtggtaga gcgtgtagaa cgtgccgctc gcgcagtcga agggcatgtc
     7981 gaactcgccg cccatgttgt cgccctcggc gggatagacg atgtgcactg tcttctcgtc
     8041 cttcagcgac cagttgaact tgtccggcgt cttgccgtcg gccttgtcgt cgtgaccggt
     8101 cttgtccgcg ttgaagacca tctgtttggc cttcccgccg tcgagatact cgtacgtgcc
     8161 gacgaggcag tcgcccttag cgccggcttt cgctgcgggt gcgccgccac cggtcgctgc
     8221 aggtttgtct tctttcttcc cgccacaccc gacaacgccc agggtcacga taaacatcaa
     8281 agtgagcttg gtcatggcct gcgattctag ccgttacact agacgaatga gatgtcggct
     8341 ggcatggatg ctcgtgtcca tccacgcgct cgcagggtgc ggccggctcg cattcgatgc
     8401 caacgccgac gccgacgcca acgccgacgc gcccgtctgc accgccaaca tcgatctcga
     8461 ccgtgactcg accaactgcg gcgcgtgcgg tcacgactgc ctcggtggtg tgtgcgagca
     8521 aggtgcctgc caacccatcg cgctcgatac ctcgagtaac gagctgcgtt ccatcgtgct
     8581 cgacgacacg tacctctact tcgccggcgt cgcctcgatc ggtcgcattc ccaaatcggg
     8641 cggtgcggtc cagaccgtcg cgaccttcag cggcggcgca tttcggatga ccgagcacgc
     8701 cggctacctc tacttcacga tcccagacgc actccaaatc gcgcgcacgc cgatgacgtc
     8761 gcaggccgcg cccgccgaaa tcatcgacac gaacatctcg ttcctcagtg gcatcgagag
     8821 cgacgggaca tacgtttact gggcgacaac ggagtcgacg gcgcgcgtgc gccgcaccga
     8881 gatcgcgaca gggatccgca gcgacgtcac gacgaccgac ggcggtccga tacacggcct
     8941 gcgtctcgtt ggttcgacgc tctatttcct gcacgagagt gtcggtctcg agtcgatcca
     9001 tgcagacggc agcagcggct ctatcgttgc gcccgcgggg acgttcgagg cggagatcga
     9061 cggcggcgtc gcgttcatga ccagcctcac gcgtgacgcg atcgtgcgcg tgtcgctcgc
     9121 cgacggtgca gttacgccgc tcgccccagc cgacggtccg tggggcctcg ccatcgacga
     9181 caccaacgtc tacgtggcga acgaggtggg cggctggatc ggccgcgttg cgcgcacggg
     9241 cggcacacag cagcaagtcg tcctcgcgtc agtaaccgat gcggtcggcg tcgccgtcga
     9301 cgacaaggcc atctactaca cggcgcgcta cggcggcatc tacaagctcg cgaagtagcc
     9361 tgcgtcgggc aacgctcact gcggtcgcag aatgacgacg accggattga tctttccgac
     9421 gttgccacct gcggggtacg cgtacgacgc aaaagtcgcg agcccccaga ccatgatcga
     9481 gaaggcgccg ccgctcgtcg ccgtgtgccg tccgttcgtg cacgtgccga ctggggtcac
     9541 gcgctgaagg tcgacgttgg tgatctggta ctcgctgctt cccggcacgg gctgccagcc
     9601 ggaaatcacg ccgaggcaat cgaccgtgac atcttggaag ctaccgccct gcgccttgcg
     9661 cgtgacgacg agattcgtcg tcgtgtacgt cgggtcggtg aagaatacgt agctcgacag
     9721 ccactgcgcg ggcggcaaga tattgacgta ttcctcgtcg ccgaggcact gcgtgccggt
     9781 gatctgatcg gccaccgagc ccggacggct accgccagaa accatgcagc ccgccatgta
     9841 ctggccgacg tagaacggat gcatgtcgtc ctggctcgtg atcgtgaacg cgcccgttgc
     9901 ctgaaactgg agaacctgac cgcgattgag cgtggccggt gcgccggtta ccgcggacga
     9961 gtacgtgagc gtcgtcccat cggcagcgcc gacaagacgg tagacgatcg actcgggttg
    10021 caggttcgcg cgccgcgttg cgtgcggcgg aatcacatac tgatacccga gcgccgaaac
    10081 gggtggcacg agttggtggc ccgagtcgca gccgccgccc gacgacgtcg tggtcgactg
    10141 gcacaagtac gtggtaccgc cagtaaacga aaccggcttg tccgattgga tcacgctgcc
    10201 gcccatctcg ccggtcgcct gccactggat gtactgcccc gcctgcagcg tgtacgtcgt
    10261 cgtcgtgctc gccggtgccg cggccacacc gctgccggcg ggcaacgccc tcgtcggcga
    10321 gatcatcacc gtcgtattgt ccgcggcagc gacgagctgc ccccagcctg cgccactcgt
    10381 cggcgcgggc ccgcccgtca catagtttgt gccccaagca cttgtcggca gcagcagctc
    10441 cgcgctcggc aagaacgagg tcgccgcgcc gtacggcatg atgtcgtacg ccgagaccgg
    10501 cacgatcgtc gtgatgtgaa acgccgtgcc gatgccggta gccgaatcac cgccggtcca
    10561 caccgcgctg ccgcccgtgc ccgacacggc cggcggcaca ggacacgtca tcggaatggt
    10621 ggcgttcgcg gccgccggat catggctcaa gaacaggacg ccgacgttcg agctcgcgat
    10681 gcccgtcgcc gcgatcgtcg gccacgtcgc cgcatcgttg ctgccgttcg gcacgcgtcc
    10741 aaacatggtc gcatcgaacg tctgagtgcc gcgcgtgatc gtaacggtcg catcgctcgg
    10801 ccaattgttc gcgaggaaga ccgcaaagca cggcggcgag atgctcgaat aaaacgacgg
    10861 cgtcgcaacc acgaagtcgc agccgaccga gccctgactc gctgccgccg cctgacacgc
    10921 aggcacgcac gcgcccgccg cacagccctg atcaggtgga cacgtgccga caacgttgcc
    10981 cgcatcgtcg aggacctcgt gcagatccga gctgcactgg gtccccatag gaagcgcatc
    11041 accgccgttg ccgccatcgc cgtccgagtc gcgcacagac ggaccgcagg ccatgagcag
    11101 agtcactgca aatacgagcc tcatcgaggc cgactgtaag ccggggctgc cgaccgtcgc
    11161 cacaaccaaa atcgagtcat ccgaaacgcc ggctcgacga gctactgccc ggcccgtggg
    11221 cgcgcgctcg cgccgacgcc tagcctgcgt cggtgatcgg gccggaagtg ctacgccgag
    11281 cgccgcggcc tgtggtcttc cttagcgaaa ctcgagtccg tgacgcgcag cgatcgcgcg
    11341 catgtcgtgg gcggggccgc catccggcga gcccgtatcg acgttcgcgg cgcagatctc
    11401 ggcgacacct gcttcgaaga cgcgagccgg gctgctgatc gtcagcaggc gccccggttg
    11461 cggcgacgtg caacggaacc cgtgccacgc gccccgcggt atcacgatca cgtcgcaggg
    11521 tccgacatca tgggtcacag cgtcgacctc gaaggtgaac gtcccctcga taacgatgaa
    11581 cgtctcctga aatccgggat ggcgatgaac gcctgcaccc gcgccgaccg gttctgcggt
    11641 ttcgaacagg ctgtacgacc cctcgtcctc gccgtcggtc cgtttgaacg atatcgcaag
    11701 ccgcccgagt tgatacttct tgccctccga cgcccgaaat acctgcgtcg tcttcgtgtt
    11761 gctcatcgca gcacgctacc gcaatcgtca gccgacggtt cgccgcgcct catcactcgc
    11821 cacagtggcg caacgcctct ctgatcaggg cgccgaggtc gacgtccgtg cccacgtggg
    11881 cacaagcctc ctcgaccgca cgcgccgcga gtgctgcctt gaagccgagc tgccgaagcg
    11941 cggcctgcgc cagcgtcgcc ggatcatcca tctcgagatg gggcggcgcg ccgctcgctc
    12001 gccccacgac cgatcgcacg atgaccgatc gcacgactag tgatcgcgcg acgagcaatc
    12061 gcgcgacgag ccgcgcacgc gtcattgctc tgttctcgcg cgtcggcagt cgggactcga
    12121 acccggcgaa tacccaagaa gcggaacaag cgaccgtttg cccgcttgtt tcgtgcgtgc
    12181 cttcactact cgccgcagtg gcgca
//
LOCUS       DATLBZ010000152        12313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3870112, whole genome shotgun sequence.
ACCESSION   DATLBZ010000152 DATLBZ010000000
VERSION     DATLBZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12313
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3870112"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..672
                     /locus_tag="VMZ53_08085"
     CDS             163..672
                     /locus_tag="VMZ53_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28452.1"
                     /translation="MKRLALALLVGCGSSTSGSTTNDNASDAGACVADLVAPGGACPS
                     QCTSCDGNVCKIDCASGACNDTTIDCPDGFNCEITCSGLDSCDTTVVHCPAKYACAMK
                     CTSYDSCGDVVQHCGLGACTMECNGPTEACGGSTIDCAPGGACATTCKNGSHGASLDC
                     RGACSCTPC"
     gene            complement(669..2042)
                     /locus_tag="VMZ53_08090"
     CDS             complement(669..2042)
                     /locus_tag="VMZ53_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS28453.1"
                     /translation="MEHLSGTRIRREATPATVNLRKCKLVVIKGTQRGTEFVISGDVI
                     RIGKAPENDLVLADETVSRVHFEIVRDAKGYLVRDLKSTNGTFLDSAEVKEAYLRAGS
                     VIAVGATELKFTPFEERIEILPSEKEALGEMVGKSPAMREIFGLIERIAPTDATVLIE
                     GETGTGKDMIARTLHQLSPRGEKPFIVVDCGAVAGTLIESELFGHEKGAFTGAVAARQ
                     GAFELASGGTVFLDELGELSLDLQPKLLRVLEQREFRRVGGQKTIKVDVRIIAATRKD
                     LRSEVEKGKFREDLYFRLNVVPITAPSLRERREDIPLLIDYLLKKLAPDGAALSDATR
                     AALMAHDWPGNVRELRNVIERALALGADPGMLVAPLGPDASSAKGVQLRDALEFEPGL
                     SFRDTKEKWNELFERRYLTWLIKRADGNISKAARDADMDRKYLHKLLRKYGITAGGAD
                     APDDDDN"
     gene            2103..2795
                     /locus_tag="VMZ53_08095"
     CDS             2103..2795
                     /locus_tag="VMZ53_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HUS28454.1"
                     /translation="MLRTLGGIVLAIAAWSSVAHAQPAAPTKPSEAPLPSCLDQTIAE
                     QVGEKLKPRGVQKRDFMKRRKLNLVTHAGLFGGDLTSSSWVAGGQLGFFFTEDFGISG
                     EFDLTPLTLDLDAPLNKFFGDNRFEPGMAYLVLGNFMWSPIHAKLKLGGGIVHADIMF
                     VAGAGRMIHDAVQGVTFNAGASIDFFVTKVVTIRIQTRDVMAVEEIAAETRYTNNLVA
                     TAGLSWWIPVGL"
     gene            2792..3508
                     /locus_tag="VMZ53_08100"
     CDS             2792..3508
                     /locus_tag="VMZ53_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HUS28455.1"
                     /translation="MKRALVTLLGLIALTGVAHAEEKAAEKQVAAKDAVEQSRTTEIC
                     IDQEIADRLAIKRKRRGAIDRLFVKQGRHEITAGGGYYASDLLSGTYLVGGSYTYHMT
                     DETAVEFAGWWTHANADIIRALEDQRGVVLTDTYARMIFAESLLVWSPVYGKLRLGGS
                     IVHFDLHADLGVGVIDSKTSRGAAGVVGFGIKLFLGQAVAIRVDARNRTFRQDLLDER
                     FLVNDSAITAALSVFLPFSN"
     gene            3532..4425
                     /locus_tag="VMZ53_08105"
     CDS             3532..4425
                     /locus_tag="VMZ53_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28456.1"
                     /translation="MRRGLLIALLLVPALALAGPKRRPKKKAAPPPPAEETEKPAEAT
                     PPPTVPSVDMSGGLGTTKGTTPTTTTPASGTSTVPSGATTPKAAGTSSTVPASTPATP
                     PTGAGSGSASPQPPTEPVEKEPKADGKDVDVDSLRQEYLSLRDELFKSRARANAVASQ
                     LYSTRVTIKFTWGSARYYGVSKASIRLDGATVYEDASGAIAGDDGVRFDGYVAPGRHL
                     ITFHVEATGKDDDTFTSSTESQIVVKAVANKDLVIAAKGHDSGDIAYAWKRSEKGSYG
                     LGIDVGVKTVASGTAAAKGKK"
     gene            4422..6437
                     /locus_tag="VMZ53_08110"
     CDS             4422..6437
                     /locus_tag="VMZ53_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28457.1"
                     /translation="MKAFALAAIASIALGGSAFAKGGKGAATESSGAEAKSVDDPIAK
                     YFTELEALKLIDVESGSLESLRKELQAAEQLLKDGAFTNAAVALYAIVKSPRYSAFTD
                     FVEFENAEYDLAVALERSGAYGASMEIIEQILRRGPAAPYWGPAHRRAVDIAIETRDH
                     AGILARIEAIKSSDSIPPSASGERAYLQGRVAYEKNKLADAEGWLIQITRKSRLFSSA
                     VYLRGVIRARRGEYKEAAEAMCEVAGTPDNDKLTFVVDDRYFTVKDLARLGLGRIAHE
                     QNEYDDAYYHYFQIPDDSVYLPDALFEAAWSMYQKRELDTARDLTAEFLKNFPTSPLW
                     PEASLLAGYIELADCKFDDSQKWYDDLVAKLQPIVIEMDKVRKDPDARRRLFATALTR
                     YREVKTTGEIAGAKPGSEKAETPYDQIVALLRLDPKFLRLNDAVTGSGELADSAPQVT
                     RQWQNLAAQVKETKVAAISSTKTLEQEQLADANAVLEEYRRLAKQLSVQQDEIARAKR
                     EKTMPADEAAKELDRLDDVSIKVQVALANAQATADAAAKAAAGDASTGIRPMIQSDIN
                     EARRLDKASHELAGRLAAEGEKLAAQSLDRLYDDTRKVLDKAKLGKVDAIIGQKRKLD
                     IEVQDLAAGRFPEELIGRLWNASMIGDDEEYWPFEGVYWADEYSGWR"
     gene            6437..9586
                     /locus_tag="VMZ53_08115"
     CDS             6437..9586
                     /locus_tag="VMZ53_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS28458.1"
                     /translation="MLKRALLSLALLAPSIAYAQKAAPQELDIQRGPAAELYIRKRPP
                     TPEAPVLSEELRKLLNSKEKKRDDKRLEAIGLLEAFLQSNPTADAKADGMFKLAELLW
                     EESRRLYLIAMEGYGRDLEKCSQKKGECEQPKEPRIDLKKSEALYVELHDKYPGFRRM
                     DLVTYLIGFAAKEDNREDEAMAKFQEVIDKYPDSPLYGDAWMMTGEHYFAMSQWQKAM
                     DAYRHVPEVAATNDLATFKIAWCEWKLGDSMKAAADFKKVLDKRANCEKTSTSERCKQ
                     AGSLAQEALDYLVVVFTEDRSLSPKEVFDFLVSINGEQYSRDVMIKVAESYAGQAEWD
                     RSNDAFRFLIKMDPGSIKAAEYQRNIVQNWTSALDVERAQDEIKVLLDNYGPITTWAK
                     EQRNREALDRSLQTTEQLVRETAVNIHGEAQRREKLLKFPKQDGCAKKPIANAELLSL
                     YTKASDAYAQYLAAFGAAKTAAEKTIELRFYRAEILCFKLSQVEKAGDEYLEVGKTAP
                     VGKYHKDALLRAMNAFEAARPTNTAGRGQLYDVDKKFGEAIDLYATLFPADPEIVGVI
                     FKNGQKFYDYGEYDEAIKRFGVIVTKYPKDPNAGPAGDRILSALNKAQDYENIENWAR
                     KLKNAPSFAAKEQQDRLARLIVESIQKSGDKYADAGKYQEAAEFYLRVPKETNDAKTA
                     AQAMMNGGVMYEKAKEPEAAADVYVELAQKYATSAPDLAEKAAFSAGVVYEKSIFYDR
                     AAKAYELVVDKFSKGQRAPDALFNAGVLRQALGQNDRAIQHYKDYAKKFADRKDAPDV
                     AFNIGVVYQDAGDEGPAYQAFADYTRIYHSTGKRVIEAHTRAGLMALKLGQLKRAKDD
                     FSAAQALWKRSSGAEKASGKTWAAQARYNEGELVFREYEKVTLDVPPAKLEKALKQKS
                     KLLGDAEKLYLSVVDYGDLKWATAALYRIGQVYDGFAESLVNASTPKGLSADQAQAYR
                     DALDTYVVQIQMKAVDAFSTAYTKAIQLQVYDEYTAKIRESLGRLAADKFPPEREGRS
                     KERTGDRPLNPEMVTEIAR"
     gene            9643..10944
                     /locus_tag="VMZ53_08120"
     CDS             9643..10944
                     /locus_tag="VMZ53_08120"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS28459.1"
                     /translation="MHVHEKPSSSSQLDRPQLDPCESRKETEHEHEHEHEHEHEHEHE
                     HVHVTRGRGRGRGRPLAGALLLLLLAVAACGASGTRKVKGQGRTATVREPVKPAAMKE
                     FESAMRAVRLGGPEASETARARLRAALKIDSSIWEAWHDLGVIAWKEGDDEEAIDDFS
                     KALSVNRDHMPSLLARAEANRRAGKKKEAKADYKSALDQMSEDDPNRQDAAPRLASLL
                     RDSGEYEDAVEILRETVRVTGASAKIYTELGQIYIAQKRLELAQLVLAKALELDAKDP
                     AVYNSLALLAQKQGKSQEAFERFDQAASLDPNYIDARFNKASVLMDAGDYARAKAELA
                     AIVEKRPDDYAAVVSLGVAHRGLKEHAEAKKLWERVIKDAPKRSTPRADAMWNLALLK
                     IDFMEDPAGGKTDLERYLQEAPTSHSKRQDVENKCKEVKCH"
     gene            10986..11285
                     /locus_tag="VMZ53_08125"
     CDS             10986..11285
                     /locus_tag="VMZ53_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28460.1"
                     /translation="MCALVAPVAAQPKKGTPKQPAPVAGDATKTADKPADTGDKKKEP
                     KVKNFDFNALDLNGRMRTPQLLYFLERANEELERASLEKRSFIPHMVRSVEEEQL"
     gene            11282..>12313
                     /locus_tag="VMZ53_08130"
     CDS             11282..>12313
                     /locus_tag="VMZ53_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28461.1"
                     /translation="MTTVALRTALVWNGEVMDDVVSHEPKSITVGNAGGATFTVPNVG
                     LPKNFAIVRPGNRGYLLTLGSKMRGTISLDGERKEVEELVRGQAGGFFATPISGKDWG
                     VVDLDETGAYQLFFQFVPVEDAEPFLAKPVLYAGVAGYLISMFALAGIFFLRGVHSDF
                     MLFDSGTLGDFAECLFRGWLLSTIVLGIAWLVAWVFKLDGDSQASIAFSIILHATLLF
                     MTYQLYDNSDPFVWPGPRSATGNYLVTRLEPEEEKEPPKPVLATISKNDSGAAAPKTP
                     DKPKNTATKNDEGASGGKGEVERARDPNAKDVPAAPPKVALFEDKNRRVLDNIIDRNL
                     ETSLTKFNGI"
BASE COUNT         2433 a         3965 c         4002 g         1913 t
ORIGIN      
        1 gcccgcaggc tcgtacgtcg gctgcctgca gacgcgtgac ttctccgcgc tcgagccgga
       61 gatcgaagag aacaagtggt actgccccgg tgtcggtcag gtgcgcgcga tcgcgacaaa
      121 gggcggcgcc gagaccgagg atctgctttc ggtgacgcat ccatgaagcg gctcgcgttg
      181 gcgctcctcg tcggatgcgg cagcagcacg agcggcagca cgacgaacga caatgcgtcg
      241 gatgcaggtg cgtgcgtcgc cgacctcgtc gcgccaggcg gcgcatgtcc ttcgcagtgc
      301 acgagctgcg acggcaacgt ctgcaagatc gactgcgcgt ccggtgcatg caacgacacg
      361 acgatcgatt gccccgacgg cttcaactgc gagatcacgt gcagcggtct cgactcgtgc
      421 gacacgacag tcgttcactg ccctgcgaag tacgcgtgcg ccatgaaatg cacgtcgtac
      481 gactcgtgcg gtgacgtcgt gcagcactgc ggcctcggcg cgtgcacgat ggagtgcaac
      541 ggtccgacgg aagcgtgcgg cggctcgacg atcgactgcg cgcccggcgg cgcatgcgcg
      601 acgacttgca agaacggctc gcacggtgcg tcgctggact gtcgcggcgc gtgcagctgc
      661 acgccctgct aattatcgtc gtcgtcgggg gcgtcagcgc cgccagcggt gatgccgtat
      721 ttgcgcagca gcttgtgcag atacttgcga tccatgtcgg cgtcgcgcgc ggccttcgag
      781 atatttccgt cggcgcgctt gatgagccac gtcaggtagc ggcgctcgaa cagctcgttc
      841 cacttctctt tcgtgtcgcg aaacgagagg ccgggctcga actcgagcgc gtcgcgtagc
      901 tgcacgcctt tcgcggagct cgcgtcggga ccgagcggcg cgacgagcat gcctggatcc
      961 gcgccgagag cgagcgcgcg ctcgatcacg ttgcgcagct cgcgaacgtt accgggccag
     1021 tcgtgcgcca tgagtgcggc gcgcgtcgcg tcggagaggg ccgcaccgtc gggcgcgagc
     1081 ttctttagca ggtagtcgat cagcagcggg atgtcttcgc ggcgctcgcg cagcgatggc
     1141 gcggtgattg gcaccacgtt gaggcggaag tagaggtcct cgcggaactt gcctttttcg
     1201 acctcgctgc ggaggtcttt gcgcgtcgcc gcgatgatgc ggacgtcgac cttgatggtc
     1261 ttttgcccgc cgacgcgacg gaactcgcgc tgctcgagga cacgcagcag ctttggctgc
     1321 agatcgagcg acagctcgcc gagctcgtcg agaaagaccg tgccacccga cgcgagctcg
     1381 aacgcacctt ggcgagcggc gactgcgccg gtgaacgcgc ccttctcgtg gccgaacagc
     1441 tcgctctcga tcagcgttcc cgccaccgcg ccgcaatcga cgacgatgaa gggtttctca
     1501 ccgcgcggcg agagctggtg cagcgtgcgc gcgatcatgt ccttgcccgt gccggtctcg
     1561 ccctcgatga ggacggtcgc gtcggtgggc gcgatgcgct cgatgaggcc gaagatttcg
     1621 cgcatcgccg gcgacttgcc gaccatctcg ccgagcgctt ctttttcgga cggcaggatc
     1681 tcgatgcgtt cttcgaacgg agtgaacttg agctccgtcg cgccgactgc gatcacggag
     1741 cccgcgcgca ggtaggcctc tttgacttcc gcgctgtcga ggaacgtgcc gttcgtgctc
     1801 ttgagatcgc ggacgaggta gcccttcgcg tcgcgcacga tctcgaagtg gacgcgcgac
     1861 acggtttcgt cagcgagcac gagatcgttt tctggagcct tgccgatccg gatcacgtcg
     1921 cccgagatca cgaactcggt gccgcgctgc gtgcctttga tgacgacgag cttgcacttc
     1981 ctcaggttga ccgtcgccgg cgtcgcttcg cgccggatac gtgtccccga gagatgctcc
     2041 acagcggaat cgtaccacgc gcaacgtgcg aacaaggctg aggatgttgt acgctcgccg
     2101 cagtgttacg cacgcttggg ggcatcgtgc tggcgatcgc tgcgtggagc agcgttgcgc
     2161 atgcacagcc tgctgcaccg acgaagccgt cggaggcgcc tctgccctcg tgtctcgatc
     2221 agacgatcgc agaacaagtt ggcgagaagc tgaagccgcg cggcgtgcag aagcgcgact
     2281 tcatgaagcg ccgcaagctg aacctcgtca cgcacgcggg cttgttcggc ggcgacctga
     2341 cgtcgtcgag ctgggtcgcc ggcgggcagc tcggcttctt tttcaccgag gacttcggca
     2401 tctccggcga attcgatctc acgccgctca cgctcgatct cgacgcgccg ctcaacaagt
     2461 tcttcggcga caaccgcttc gagccgggca tggcgtacct cgtgctcggc aacttcatgt
     2521 ggtcgccgat ccacgcgaag ctgaagctcg gcggcggcat cgtgcacgcg gacatcatgt
     2581 tcgtcgcggg tgcgggccgc atgatccacg atgccgtgca gggcgtgacg ttcaacgccg
     2641 gcgcatcgat cgacttcttc gtcacgaagg tcgtcacgat tcgcatccag acgcgcgacg
     2701 tcatggccgt cgaggagatc gcggcggaga ctcgctatac gaacaacctc gtcgcgaccg
     2761 cgggcctgtc gtggtggatt ccggtgggcc tatgaagcgc gcgcttgtca cgctgctcgg
     2821 gctcatcgcg ctcaccggcg tcgcacacgc cgaggagaaa gcggccgaga agcaggtcgc
     2881 tgcgaaggac gcggtcgagc agagtcgcac gaccgagatc tgcatcgacc aagagatcgc
     2941 ggaccgcctc gcgatcaagc gcaagcgacg cggtgcgatc gatcgcctgt tcgtgaagca
     3001 aggccggcac gagatcacgg cgggcggcgg ctactacgcg agcgatctgc tcagcggtac
     3061 gtacctcgtc ggcggctcgt acacgtatca catgacggac gagacggcgg tcgagttcgc
     3121 gggctggtgg acccacgcga acgccgacat cattcgcgcg ctcgaagatc agcgcggcgt
     3181 cgtgctcacc gatacgtacg cgcggatgat cttcgccgag tcgttgctcg tgtggagccc
     3241 ggtgtacgga aaactccgac tcggcggctc gatcgtgcac ttcgatctgc acgcggacct
     3301 cggcgtcggc gtgatcgact cgaagacgag ccgcggcgct gccggtgtcg tcgggttcgg
     3361 gattaaactg ttcctcgggc aggcggtggc gatccgtgtg gacgctcgca accgcacgtt
     3421 ccgtcaggac ctgctcgacg aacgttttct cgtgaacgac agcgccatca ccgctgctct
     3481 gtctgtgttc ttgccgttca gtaactgact gcggagaagc ccatgccccg gatgcgaaga
     3541 ggtctcctga tcgcgctgtt gctggtaccc gcgctggcgc tggctgggcc caagaggcgt
     3601 ccgaagaaga aggccgcgcc gccgcccccg gccgaggaga ccgagaagcc ggcagaggca
     3661 acgcctccgc cgaccgtccc gagcgtcgac atgagcggtg ggctcggcac gacgaagggc
     3721 acgaccccga cgacgacgac gccagcgtct ggcacatcga ccgttccgag cggcgcgacc
     3781 acaccgaaag ccgccggcac ctcgtccacc gttcccgcca gcacgcccgc cacgcctccc
     3841 accggcgcag gctcgggctc cgcgtcacca caaccaccga cggagcccgt cgagaaagag
     3901 ccgaaggccg acggcaagga cgtcgatgtc gattcgctgc ggcaagagta cttgtcgctg
     3961 cgcgacgagc tgttcaagtc gcgcgcgcgt gcgaacgccg tcgcgagcca gctctactcg
     4021 acgcgcgtga cgatcaaatt cacgtggggc tccgcccgct actacggcgt gtcgaaggcg
     4081 tcgatccgcc tcgacggcgc gacggtctac gaagatgcat caggcgcgat cgccggtgac
     4141 gacggcgttc gcttcgacgg ctacgtcgct ccaggtcgcc acctgatcac gttccacgtc
     4201 gaggcaacgg gcaaggacga cgacacgttt acgtcgtcga ccgagagcca gatcgtcgtg
     4261 aaggcggtag ccaacaagga tctcgtgatc gcggcaaagg gccatgacag cggcgacatc
     4321 gcgtacgcgt ggaagcgcag cgaaaagggc agctacggcc ttggcatcga cgtcggcgtg
     4381 aagaccgttg cgtcgggcac cgcggcggcg aagggcaaga agtgaaggcg ttcgcgctcg
     4441 cagcgatcgc gagcatcgcg cttggcggct cggcgttcgc gaagggcggc aaaggtgcgg
     4501 cgaccgaatc ctcgggcgcg gaggcgaaga gcgtcgacga tccgatcgcg aagtacttta
     4561 ccgagctcga ggcgctaaag ctcatcgacg tcgagagcgg aagcctcgag tcgctgcgca
     4621 aggagttgca agccgccgag caactgctca aagacggcgc gttcacgaac gccgcggtcg
     4681 cgctctacgc gatcgtgaaa tcgccgcgct acagcgcgtt caccgacttc gtcgagttcg
     4741 agaatgccga gtacgacctt gcggtcgcgc tcgagcgctc gggtgcgtat ggcgcgtcga
     4801 tggagatcat cgagcagatt cttcgtcgtg gacctgcggc gccgtattgg ggacccgcac
     4861 atcgccgcgc cgtcgacatc gcgatcgaga cgcgcgatca cgcgggcatc ctcgcgcgca
     4921 tcgaggcgat caagagcagc gattcgatcc cgccgtcggc gagtggcgag cgcgcctatc
     4981 tccagggccg cgtcgcatac gaaaagaaca agctcgccga tgcggaaggc tggctcatcc
     5041 agatcacgcg caagagccgg ctgttttcgt cggccgtgta tctgcgcggc gtgattcgcg
     5101 ccaggcgcgg cgagtacaaa gaggccgccg aagcgatgtg cgaagtcgcc ggcacgccgg
     5161 acaacgacaa gctgacgttc gtcgtcgacg atcgctactt taccgtgaag gacctcgcgc
     5221 gccttggcct cggccgcatc gcgcacgagc agaacgagta cgacgacgcg tactaccact
     5281 acttccagat tcccgacgac agcgtgtatc tacccgacgc gctgttcgag gccgcgtggt
     5341 cgatgtatca gaagcgcgag ctcgacacgg cccgcgacct caccgcggag ttcttgaaga
     5401 acttcccgac gtcgccgctg tggcccgaag cgagcctgct cgctggctac atcgagctcg
     5461 ccgactgcaa gttcgacgat tcgcagaagt ggtacgacga cctcgtcgcg aagctgcagc
     5521 ccatcgtcat cgagatggac aaggtgcgca aagaccccga cgcgcgccgc cggttgttcg
     5581 cgacggcgct cacgcgctac cgcgaggtga agaccaccgg cgagatagcc ggcgcgaagc
     5641 ccggcagcga gaaggccgag acgccctacg atcagatcgt cgccctactc cgcctcgatc
     5701 cgaagttcct gcgcctcaac gacgccgtca cgggatccgg cgagctcgcc gatagcgcac
     5761 cacaagttac gcggcagtgg cagaacctcg ccgcgcaggt caaagaaacc aaggtggcgg
     5821 cgatcagcag cacgaagaca ctcgagcaag agcagctcgc ggatgcgaat gcggtgctcg
     5881 aggaataccg ccgcctcgcg aagcagctct ccgtgcagca agacgaaatc gcccgcgcca
     5941 agcgcgagaa gacgatgccc gctgacgagg ccgcgaagga gctcgaccgc ctcgacgacg
     6001 tgagcatcaa ggtccaggtc gcgctcgcaa atgcgcaagc gaccgcagat gccgcagcga
     6061 aggccgccgc cggcgacgcg agcacgggca tccgcccgat gatccagagc gacatcaacg
     6121 aagcgcgccg gctcgacaag gcatcgcacg agctcgcggg tcggcttgcc gccgaaggcg
     6181 agaagctcgc cgcgcagagc ctcgataggc tctacgacga cacgcgcaag gtcctcgaca
     6241 aagcgaagct cggcaaggtc gacgcgatca tcggccagaa gcggaagctc gacatcgagg
     6301 tccaagacct cgccgcaggt cggttccccg aggagctgat cggcaggctc tggaacgcat
     6361 cgatgatcgg cgacgacgaa gagtactggc cgttcgaggg cgtgtactgg gccgacgagt
     6421 actcggggtg gcgctgatgt tgaagcgcgc cctgctctct ctcgctttgc tggcgccgtc
     6481 gatcgcgtat gcgcagaaag ccgcgcccca agagctcgac atccagcgcg ggcccgcggc
     6541 ggagctgtat atccgcaagc gcccgccgac gcccgaagcg cccgtgctct ccgaggagct
     6601 ccgcaagctt ctcaattcga aggagaagaa gcgcgacgac aaacggctcg aggcgatcgg
     6661 cctgctcgaa gcgttcttgc aatcgaaccc gaccgccgac gccaaagccg acggcatgtt
     6721 caagctcgcc gagctgctgt gggaggaatc gcgccgtctc tacctgatcg cgatggaggg
     6781 ctacggccgc gacctcgaga agtgctcgca gaaaaaaggt gagtgcgagc agccgaagga
     6841 gccgcgcatc gatctcaaga agagcgaggc gctgtacgtc gagctgcacg acaagtaccc
     6901 cggcttccgc cgcatggatc tcgtcacgta cctcatcggc ttcgccgcca aggaggacaa
     6961 ccgcgaagac gaggcgatgg cgaagttcca agaggtcatc gacaagtatc cggactcgcc
     7021 gctctacggc gacgcgtgga tgatgacggg cgagcactac ttcgcgatgt cgcagtggca
     7081 gaaggcgatg gacgcctatc gccacgtgcc cgaggtcgca gcgacgaacg acctcgcgac
     7141 cttcaagatc gcctggtgcg agtggaagct cggcgactcg atgaaggccg ccgcggactt
     7201 caagaaggtc ctcgacaagc gcgcgaactg cgagaagacg agcacgagcg agcgctgcaa
     7261 gcaggccggc agcctcgcgc aggaagccct cgactacctc gtcgtcgtgt tcaccgagga
     7321 tcgctcgctg tcgccgaagg aagtgttcga cttcctcgtc tcgatcaacg gcgagcagta
     7381 ctcgcgcgat gtcatgatca aggtcgccga gagctacgcc ggccaggccg agtgggatcg
     7441 cagcaacgat gcgttccgct tcctcatcaa gatggacccc ggctcgatca aagccgcgga
     7501 gtaccagcgc aacatcgtcc agaactggac gagtgccctc gacgtcgagc gcgcgcaaga
     7561 cgagatcaaa gttctcctcg ataactacgg cccgatcacc acgtgggcga aagagcagcg
     7621 caaccgcgag gccctcgatc gctcgctgca gaccaccgag cagctcgtgc gcgagaccgc
     7681 ggtgaacatc cacggcgaag cgcagcgccg cgaaaagctg ctcaagtttc ccaagcaaga
     7741 tggctgcgcg aagaagccga tcgcaaatgc cgagctgctc tcgctgtaca cgaaggcgtc
     7801 cgatgcgtac gcgcagtatc tcgcagcgtt cggcgccgcg aagaccgccg ccgagaagac
     7861 gatcgagctg cgcttctatc gagcagagat cctctgcttc aagctctcgc aggtcgagaa
     7921 agcgggcgac gaatatctcg aagtcggcaa gaccgcgccc gtcggcaagt accacaagga
     7981 cgcgctgctc cgcgcgatga acgcattcga ggcggctcgc ccgacgaaca ccgccggtcg
     8041 cggccagctc tacgacgtcg acaagaagtt cggcgaagcg atcgatctct acgcgacgct
     8101 gttccccgct gatccagaga tcgtcggcgt catcttcaag aacggccaga agttctacga
     8161 ctacggcgaa tacgacgagg cgatcaaacg cttcggcgtc atcgtcacga agtacccgaa
     8221 ggatccgaac gcgggccccg ctggtgaccg catcctctct gcgctcaaca aggcgcagga
     8281 ctacgagaac atcgagaact gggcgcgcaa gctcaagaac gcgccgtcgt tcgccgcgaa
     8341 ggagcagcaa gaccgcctcg cgcgcctgat cgtggagtcg atccagaaga gcggcgacaa
     8401 gtacgccgac gccggcaagt accaagaggc cgccgagttc tacctgcgcg tgcccaaaga
     8461 gacgaacgac gcgaagaccg ccgcgcaagc gatgatgaac ggcggcgtga tgtacgaaaa
     8521 ggcgaaggag cccgaggcgg ctgccgatgt ctacgtcgag ctcgcgcaga aatacgcgac
     8581 gagcgcgccg gaccttgcag agaaagccgc gttctccgct ggcgtcgtct acgagaagtc
     8641 gatcttctac gaccgcgcgg ccaaggcgta cgagctcgtc gtcgacaagt tcagcaaggg
     8701 tcagcgcgca ccggacgcgc tcttcaatgc aggcgtgctg cgccaggcgc tcggccaaaa
     8761 cgatcgcgcg atccagcact acaaggacta cgcgaagaag ttcgccgatc gcaaagacgc
     8821 acccgacgtc gcattcaaca tcggcgtcgt ctaccaagac gcgggcgacg aaggcccggc
     8881 gtaccaggcg ttcgccgatt acacgcgcat ctatcactcc acgggcaagc gcgtcatcga
     8941 agcgcacacg cgtgctggcc tgatggcgct caagctcggc cagctcaagc gcgcgaagga
     9001 cgacttctcg gcggcacagg cgctgtggaa gcgctcgtcg ggcgcggaga aggcgtcggg
     9061 caagacgtgg gccgcgcaag ctcgctacaa cgagggtgag ctggtcttcc gcgagtacga
     9121 gaaggtcacg ctcgatgtcc cgccggccaa gctcgagaag gcgctcaagc agaagagcaa
     9181 gttgctcggc gacgccgaga agctctacct ctccgtcgtc gactacggcg atctgaagtg
     9241 ggcgacggct gcgctgtatc gcatcggtca ggtctacgac ggcttcgccg agtcgctcgt
     9301 caacgcatcg acgccgaagg gcctctcggc ggatcaagcg caggcatatc gcgacgcgct
     9361 cgatacgtac gtcgtgcaga tccagatgaa ggccgtcgac gcattctcga ccgcgtacac
     9421 caaagcgatt cagctccagg tctacgacga gtacaccgcg aagattcgcg agtcgctcgg
     9481 ccggctcgcc gcggacaagt tcccgccgga gcgcgaaggc cgcagcaaag agcgcaccgg
     9541 cgatcgcccg ctcaaccccg agatggtgac ggagattgcg cgatgagggc gagcaacgac
     9601 atgggtcgtc gtacccgtcc ccgtgtcacg tgtcacgtgc acgtgcacgt ccacgaaaag
     9661 ccctcgagct cttcgcaact cgatcgtccg caactcgatc cctgcgagtc ccgcaaggaa
     9721 accgagcacg agcacgagca cgagcacgag cacgagcacg agcacgagca cgagcacgtg
     9781 cacgtgacac gtggacgtgg acgtggacgt ggacgaccgc tcgccggcgc gctgctgctg
     9841 ctcctccttg cggttgccgc atgcggcgct tcgggaacgc gcaaggtgaa aggccaaggc
     9901 cgcaccgcga ccgtgcgcga gcccgtgaag cccgccgcga tgaaagagtt cgaatcggcc
     9961 atgcgcgcgg tgcgcctcgg cggccccgaa gcgtcggaga ccgcgcgtgc tcgcttgcgc
    10021 gccgcgctga agatcgactc gtcgatctgg gaggcgtggc acgatctcgg cgtcatcgcg
    10081 tggaaagaag gcgacgacga ggaggccatc gacgacttct cgaaggcgct ctccgtcaat
    10141 cgcgatcaca tgccatcgct gctcgcgcgc gcggaagcga atcgccgcgc cggcaagaag
    10201 aaagaagcga aggccgacta caagtcagcg ctcgatcaga tgagcgaaga cgatccgaat
    10261 cgtcaggacg cagcccctcg cctcgcctcg ctgctgcgcg attccggcga gtacgaagac
    10321 gcggtcgaga ttctgcgcga gaccgtgcgc gtgaccggcg cgtccgcgaa gatctatacg
    10381 gagctcggcc agatctacat cgcgcagaag cgcctggagc tcgcgcagct cgtgctcgca
    10441 aaggcgctcg agctcgatgc gaaggacccc gctgtctata actcgctcgc gctgctcgcg
    10501 cagaaacagg gcaagtcgca ggaagcgttc gagcgcttcg accaagcggc ttcgctcgac
    10561 cccaactaca tcgatgcgcg cttcaacaag gcgtccgtcc tcatggatgc gggcgactac
    10621 gccagagcca aggccgagct tgcggcgatc gtcgaaaaga gacccgatga ctacgcggct
    10681 gtcgtttcgc tcggcgtcgc acatcgcggg ctcaaggaac atgccgaggc caagaagctg
    10741 tgggaacgcg tgatcaagga tgctccgaag cgctcgacgc cgcgcgccga cgcgatgtgg
    10801 aacctcgcgc ttctcaagat cgatttcatg gaggatcccg cgggcggcaa gacggatctg
    10861 gagcgatatc tgcaagaagc tccgacgagc cactcaaagc ggcaggacgt cgagaacaag
    10921 tgcaaggagg tcaaatgcca ttgaggcata ccgccccctt tcgggggcta ttggccacgt
    10981 gcgtgatgtg cgcactggta gcacctgtag cagcgcaacc gaagaaggga acaccgaagc
    11041 agcctgcacc cgtcgcgggt gatgcgacta agaccgctga caagccagcg gatacgggcg
    11101 acaagaagaa ggagccgaag gtgaagaact tcgacttcaa cgccctcgat ctgaacggcc
    11161 gcatgcgcac gcctcaactc ttgtacttcc tcgagcgcgc gaacgaagag ctcgaacgcg
    11221 caagcctcga gaagcgatcg tttatcccgc acatggtgcg gagcgtggag gaagagcagc
    11281 tgtgacgaca gttgccctgc gcacggcgct cgtgtggaac ggcgaggtca tggatgacgt
    11341 cgtcagccac gagccgaaga gcatcacggt cggcaacgcg ggcggggcga cgttcaccgt
    11401 cccgaacgtg ggcctgccga agaacttcgc gatcgtgcgt ccaggcaacc gcggttatct
    11461 gctcacgctc ggcagcaaga tgcgaggcac gatcagcctc gacggggagc gcaaagaagt
    11521 cgaggagctc gtgcgcggcc aggccggcgg attcttcgcg actccgatca gcggcaagga
    11581 ctggggcgtc gtcgatctcg acgagaccgg ggcctatcag ctgttcttcc agttcgttcc
    11641 tgtcgaagac gccgagccct ttctcgcgaa gccggtgctc tatgcgggcg tcgctggcta
    11701 tctcatctcg atgttcgcgc tcgcaggaat cttcttcctg cgcggtgtgc actccgactt
    11761 catgctcttt gactcgggca cgctcggtga tttcgccgag tgtctgttcc gcggctggct
    11821 gctctcgacg atcgtgctcg gcatcgcatg gctcgtcgcc tgggtgttca agctcgacgg
    11881 cgactcgcag gcatcgatcg cgttctcgat catcttgcac gcgacgctgc tgttcatgac
    11941 gtaccagctg tacgacaaca gcgatccgtt cgtgtggcct ggtccgcgat ccgcaaccgg
    12001 caactacctc gtcacgcggc tcgagccaga agaagagaaa gagccgccga agcccgtcct
    12061 cgcgaccatc tcgaagaacg attccggcgc tgccgcaccc aagacgcctg acaagccgaa
    12121 gaacaccgcc acgaagaacg acgaaggtgc atccggcggc aagggcgagg tcgagcgcgc
    12181 tcgcgatccg aacgcgaagg atgtcccagc ggcacctccg aaggtcgcgc tgttcgagga
    12241 caagaaccga cgcgtgctcg acaacatcat cgatcgcaac ctcgagacga gcctgacgaa
    12301 gttcaacggc atc
//
LOCUS       DATLBZ010000153        12442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1635455, whole genome shotgun sequence.
ACCESSION   DATLBZ010000153 DATLBZ010000000
VERSION     DATLBZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12442
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635455"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /locus_tag="VMZ53_08135"
     CDS             <1..2302
                     /locus_tag="VMZ53_08135"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS28462.1"
                     /translation="GRTAALLEQERADIFTQQIGNIPPGEAVVARITIDQRLVWLPEG
                     EWELRFPTVIGPRYIGSVDTAADIRATHIKVTDKPLSASINIAIAIKDVISGGGKASS
                     PTHSLSTSSGVTELSAGARLDRDIVLRWPVAAPAPGISVATAAASQGAFGLLTIVPPS
                     RTAKPTPVPRDLIVLLDTSGSMSGGPLEKAQQVVSMMLDSLNEGDRFELIEFNMQPTR
                     YNDEPLVATAKAKAAAIKWVRSRVADGGTEMRAGVIEAMQSLRVGAQRQIVIVTDGYV
                     GGERQILEAINRRLPKSCRIHVLGVGSAVNRSLATALARAGRGVEVLVGIDDDAERGA
                     KRLLDRTSTPLLTNVEITGSAVLRHAPDRIPDVFERAPLVAALAVRPEGGQIVVRGQL
                     GTEDWEQTITVPPTKPGDGNGAILALYGRERVADVEANALFGSVDGDVEELGITFQIA
                     TRMTSWVAIDETRKVSGPTRDELVPQELPYGTSAGAFGLRGAAAPMPMQMQRTQAGMA
                     GAAMTRAGVVKSAKLDLSEAMDGDFGEDAPSEIFAEEAKKEVALDQFETEESTGQLGV
                     PAARDSANQPMSSSPPRYAPAPPRPAAGQARPSQPAAPPLGRTRAASYDADDEADERF
                     GTGSGGPTGGEDRSVSPPPAMQPAAPRPTESARGPMSRPAPEPERSADKGRAAPDDKP
                     AMPGAHRVPSDFKIGAAAPIEDTSKLPQPKQAELAKVKPRDVAKQAQEAQAPATAAAK
                     RSAWLVALIALIGLVALVLLLWWLLA"
     gene            2299..2862
                     /locus_tag="VMZ53_08140"
     CDS             2299..2862
                     /locus_tag="VMZ53_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28463.1"
                     /translation="MSQDLDIYLAAIVAGDRRAFATWLGFAEPGVRDSLRSFAAVVDV
                     EAVLQESLLRVWQVAPRFEPDGRPNGLLRLGVRIARNLAVSEVRRTKATPVDPPKLDE
                     LESTTTPTASDPLLREAIIDCRNKLPPKPRQALDSRLASGGVHDDAELAEAVGMRLNT
                     FLQNFTRARALLAECLKKRGIFFEGLT"
     gene            2859..3089
                     /locus_tag="VMZ53_08145"
     CDS             2859..3089
                     /locus_tag="VMZ53_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28464.1"
                     /translation="MTREEEDLLVEQVAGAYRPVSSGELQYHKAWLDLDEAARVRAYE
                     LARAQRPLEAALDADGLSTTARAVLARITEKM"
     gene            3211..4719
                     /locus_tag="VMZ53_08150"
     CDS             3211..4719
                     /locus_tag="VMZ53_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS28465.1"
                     /translation="MKHVLVLFALSVVAGCATEFTPKPCALDSECGNGAVCEMRGSDP
                     VCVYTDDAPIVVGQSAPISGTNQALGTAMKLGIEIAFDEQNAAGGIRGRKLQLSFRDD
                     AYQPNLAEAAARSLVDAQVSTTEIPKCPSTTNPSPGQAPVSTTAISRGPDAVIAMLGS
                     VGTPTMVRAAPVAVESGTIFFGAFTGATTILRDMQAGPCGRYIFNIRASYSQEAHASM
                     ELFKKKGVAGYKNVISFDQNDSFGQAGYDGLVAAYKDVIGNFPGSADDTNPIVRFRYT
                     RNDDASVPAQATAMQSYLAQLLANQSGTQTVGIFMTDTYGAAAELIKSLRTWQYANDS
                     QQQSLQKATRLKLYFSNVSFVGPNALAERLVAAGTVSTPSGPMPLTDNVIISQVVPNY
                     QSDSSDLIAAYNRAIAARGMKPDFTSLEGYISARVFIAGLLEHEGPFTPDGLIKTFEK
                     LPDLALGIGASSGFSPNHHQYSSSVWGTAIQPDGTFKNLYFWSDGSSIMFFE"
     gene            4716..6272
                     /locus_tag="VMZ53_08155"
     CDS             4716..6272
                     /locus_tag="VMZ53_08155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28466.1"
                     /translation="MSNEREAIQVGRYLLHRKIARGGMASIHIARLMGELGFSRIVAA
                     KRMHPELIEDQEFVAMFLDEARVASKVHHRNVVPVLDVVTLGDEVMLVQEYIHGAPLS
                     LLLQNSCSTNAHVPVPVAAAIACQVLAGLHCAHETTDELGAPLQIIHRDVSPQNIMIG
                     TDGSARLLDFGVAKAAASAHVTRKGTFKGKLGYSAPEQIRGEATRQSDVYSLGVVLWE
                     LLVGKRLHANVSGEAQLIAQIMAGKSSTITEALEAERSWIGAYRWSQLEAIEPIVRKA
                     LDPIARRRWQTAADMEEALSAAVPVASGADIAQWLKAVGKDFLEEREKMIAEEEASWR
                     SAGSSMQLPALTTVEIPGAIPTLVPTSTATEPNAMPETGASLMWKRGSTAVIFALVAL
                     LGISVAFLMRDLEHAPVAAPPPSSPPPATASVPTPPPAPDAPVVQNVVEKTVKKPAPV
                     VSSPPPVKRVTVATAPARIRAVRPPPARSAPRTPTAAPSPPKQPAVTLDCTTPFYFEG
                     RKKVFKPGCL"
     gene            6281..7255
                     /gene="purU"
                     /locus_tag="VMZ53_08160"
     CDS             6281..7255
                     /gene="purU"
                     /locus_tag="VMZ53_08160"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HUS28467.1"
                     /translation="MPAKPRTKSQTWGDESVVSPLDVGAEPTARAPKKSSLLSSRSEG
                     EMGTLMVGGPEKRGIHAALAQLLHEHGVHIVHSGQHTGGGMFFERIRFDLSQISTGRE
                     ILDASIRAMCAKWGLDYRLYGDRKKRVAILVSKYEHCLYDLLVRYRSGDLPPCEISLI
                     ISNHPDATDCAEHFQIPFRHLPVTADTKQQQEQQIIGLLRAANIELIVLARYMQILSP
                     AFVDRYPHRIINVHHSFLPAFIGANPYRQAYERGVKLIGATSHYVTSDLDQGPILEQE
                     VIRCTHRDTVEDLTRKGRDLEKRVLAAAVRLHLEDRVMVNENKTVVFE"
     gene            7277..8890
                     /locus_tag="VMZ53_08165"
     CDS             7277..8890
                     /locus_tag="VMZ53_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28468.1"
                     /translation="MRWLACAWLLVVGCFDPPTGPPYKIKLLSADPPVVSVHGGEWVT
                     IGYTGELPESYAVVVDELVGTVILEDRDAHTFTLSVPAHPDGDVLITVVNIDRDRVVA
                     SNGELLSYQRVAGMPHLTTSSSTDDTLGWDLHPELARPCARAGYVYLENIGDDPLTFA
                     AARSTNAEFTIDSAMCDALEYGEACAIGVCFSSTVPGFHAASIIVSTSAGELAASVQG
                     NVTAATAGLDPTFHGGGVVLSSGEESFYGHAIRRPDGNGIVTWSRTRGMALDVAGHEI
                     ARDLTGHIGTAPADWVIAIRAGAPGEGFDALVANTNNFAMAILVHLDDSFALVSQTDL
                     PFDSANPYYDLQRAPSGRILAIGSKAVVAIANGVVDTTYGTNGRVVLPGTYNNRSVID
                     SQGRLYAVVSSSLIRITANGTVDGSFGYGNSLLALTVDSTDRVFVVTGSNVVRLSETG
                     YGELSIPATYADDIAIDASGRIYLVGSGRVYRYSGGTQELIGYDTTYSVVCPSSGACY
                     LLGANMLTVHPFTTTAPSYYEPYVLRLAN"
     gene            complement(8887..9492)
                     /locus_tag="VMZ53_08170"
     CDS             complement(8887..9492)
                     /locus_tag="VMZ53_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28469.1"
                     /translation="MNSSTLQTAKALDPGSQRILVGGGYYASPSLDADASEATGDDVS
                     LKMPYMELGYRRGIVDKIEVGAKVTLPGTAGVDAKYQFLNAGKLAVAAGIGTGYLKIS
                     SGAEGMQTSSTIVDAIVPVYASYDVSRFFAVYSSPKYVLRYAKSVDETDMSSSSGMNH
                     LVGGTMGVRVGQKKGLFLETSYLKSVSSDFDSFQVNGSFFF"
     gene            9620..11449
                     /locus_tag="VMZ53_08175"
     CDS             9620..11449
                     /locus_tag="VMZ53_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HUS28470.1"
                     /translation="MSLLVLVAVAHSAHAEQRYAVLVGANPGWSSDRPLRYAEDDADR
                     VRDVLVTLGGFSADHVSLLRDPDTNEVRATLRDLVRSTQGSSEATMVFFYYSGHADNE
                     RVHLRGDVLTFKEIRDTLRALPSTIKLAVIDACKSGAVVRKGGSRIDDFEVSVDNPKL
                     SGMVLLTSSGADELSQESRALAGSVFTHHLVSGLRGAADANGDKQVTVAEAYHYAYAR
                     TRADTASTGTPQRPSFQYELSGQGELVLSQLKTAKTAKITLPKGNGDKYVVLDSHEMR
                     LVAEARSEKDHEVELTLAPGSYHVKKVLSDKLEVAAIVLAAGEAASTSKIAYKDAPLA
                     QGIVKGSAEDLSPDEQREWQRSQAFGLLAAGNPNAAITVFDTLLRANPGDLLSWRGRA
                     RALIRIAETYGAVNDYQREKLAMSEALKADPLLTQDPSFKSRYEKLAELDAKDQATLN
                     ARLAAEQAIRKNPRSVKHVGFGFDLLSARGMFTVDASLVVKRMIFPRVAIDVGSQGFD
                     AGVVIAPYPSRWSPFLGIGGHVSWRKMGIPVGAHSDMINVNDNMYSTEEMWGLHGRAE
                     AGAQYVGRSGFSTELGLALIAFREAGGRTVQQAWPIFHFGWVW"
     gene            11498..12025
                     /locus_tag="VMZ53_08180"
     CDS             11498..12025
                     /locus_tag="VMZ53_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS28471.1"
                     /translation="MAPQGQGDDLAELYKRHAPALHRRCASIVGNPEDARDLVQETFA
                     RYITARSTFTDKASPFTMLYRIATNASIDRLRRRKTATEEELDPEVRAGDLGREPHRV
                     DKLHDLALLTKGLTEEELTVAVLHHLDGYTQDGIAASLDISRKTVGKILAKFEDHVKK
                     RAARLGFDDRRASGT"
     gene            12015..>12442
                     /locus_tag="VMZ53_08185"
     CDS             12015..>12442
                     /locus_tag="VMZ53_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28472.1"
                     /translation="MAPEERVRKLTDLELERELAGDLPAARMAEATDADRARLGELRA
                     ESEAFLRSVDVDMEVKRIQQRVERAKPERAPAWWRFLIPAGALAAAAAVLLVFLRRGD
                     SPNGPGDDIQLKGDGVALIIHLATSGQSQQLGDGDNVKPG"
BASE COUNT         2254 a         4042 c         4089 g         2057 t
ORIGIN      
        1 gggtcgtacg gccgcattgc tcgagcagga gcgcgcagac atcttcacgc agcagatcgg
       61 caacatcccg ccgggcgaag cggtcgtcgc tcgcatcacg atcgatcagc ggctcgtatg
      121 gctgcccgaa ggcgaatggg agctgcggtt tccgaccgtg atcggcccgc gctatatcgg
      181 cagcgtcgac acggctgcgg acattcgcgc gacgcacatc aaggtgacgg acaagccgct
      241 gtcggcttcg atcaacatcg cgatcgcgat caaggatgtg atcagcggtg gcggcaaggc
      301 atcgtcgccg acgcattcgc tgtcgactag cagcggcgtg accgagctct ctgcaggtgc
      361 gcggctcgat cgcgacatcg tgctgcggtg gccggtcgcg gctccggcgc cgggaatttc
      421 ggtggctacg gctgcggcct cgcagggcgc gttcggactg ctgacgatcg tgccgccgtc
      481 gcgaaccgcc aagccgacgc cggtgccgcg cgatctgatc gtgctgctcg acacgagtgg
      541 ctcgatgagc ggtggtccgc tcgagaaggc gcagcaggtc gtcagcatga tgctcgactc
      601 gctgaacgag ggcgatcgct tcgagctcat cgagttcaac atgcagccga ctcgctacaa
      661 cgacgagcca ctcgtcgcga cggcgaaggc gaaggccgct gcgatcaagt gggtgcgctc
      721 gcgcgtcgcc gatggcggca cggagatgcg tgcaggcgtg atcgaggcga tgcagtctct
      781 acgcgtcggt gcgcagcgcc agatcgtgat cgtgacggac ggctacgtcg gtggtgagcg
      841 ccaaatcctc gaggcgatca atcgccggct gccgaagtcg tgccgcattc acgtgctcgg
      901 cgtcggctcg gcggtgaacc gctcgctcgc gacggcactt gctcgggcgg gccgcggcgt
      961 cgaagttctc gtcggcatcg atgacgacgc ggagcgcggt gcgaagcgcc tgctcgatcg
     1021 gactagcacg ccgctgctca cgaacgtcga gatcacgggc agtgcggtgt tgcgccacgc
     1081 gccggatcgc attcccgacg tattcgaacg cgcgccactc gtcgcagcgc tcgcagtgcg
     1141 tcccgaaggt ggccagatcg tcgtgcgcgg ccagctcggc accgaggact gggagcagac
     1201 gatcaccgtg ccgccgacga agcccggaga tggcaatggc gcgatcctcg cgctgtacgg
     1261 ccgcgagcgc gtcgccgacg tcgaagcgaa tgcgctgttc ggcagcgtcg acggcgatgt
     1321 ggaggagctc ggcatcacgt tccagatcgc gacgcgcatg acgtcgtggg tcgccatcga
     1381 tgaaacgcgc aaggtctctg gcccgacgcg tgacgagctc gtcccgcaag agctgccgta
     1441 cggcacgagc gcaggcgcgt ttggtctgcg cggtgctgcg gccccgatgc ccatgcagat
     1501 gcagcgcacg caagctggaa tggcgggggc tgcgatgacg cgcgcaggcg tggtgaagtc
     1561 ggcgaagctc gatctgtccg aggcgatgga cggtgacttc ggcgaggatg caccgtcgga
     1621 gatcttcgcc gaggaagcga agaaggaagt ggcgttggat cagttcgaga ctgaagagtc
     1681 caccggacag ctcggcgtgc cggctgcgag ggacagcgca aatcaaccga tgagctcgtc
     1741 gcccccgcgc tacgcaccgg cgccgcctcg tccggccgcc ggacaagctc gcccgtcgca
     1801 gcccgcggcg ccgccgctcg gaaggacgcg cgctgcgagt tacgatgccg acgacgaagc
     1861 cgacgagcgg ttcggcaccg gaagcggtgg gcccacgggt ggcgaggacc gttcggtaag
     1921 cccgccgccc gcgatgcagc ctgcggcccc gcgcccgacg gagtctgcac ggggcccgat
     1981 gagcaggccc gcgccggagc cagagcgttc cgccgacaag gggcgtgccg cgccggacga
     2041 caagccggcg atgcccggcg cgcaccgtgt gccgagcgac ttcaagatcg gtgcagccgc
     2101 gcccatcgag gacacgtcga agcttccgca gccgaagcaa gcagagctcg cgaaggtgaa
     2161 gccgcgggac gtcgcaaagc aggcgcaaga ggcgcaggca cccgcgacag cggcggcgaa
     2221 gcgcagcgcg tggctggttg cgctgatcgc gctcatcggg ctcgtcgcac tggtcctctt
     2281 gctgtggtgg ctgctggcgt gagccaagat ctcgacatct acctcgcagc gatcgtcgcc
     2341 ggtgatcggc gcgcattcgc aacgtggctc ggatttgcgg agccgggcgt gcgcgattcg
     2401 ctgcggtcgt tcgctgcggt ggtagacgtc gaggctgtcc ttcaggagtc gctgctgcgc
     2461 gtatggcagg tggctccacg cttcgagccg gatggcaggc cgaatggtct gctgcggctc
     2521 ggcgtgcgca ttgcgcgcaa tctcgccgtc agcgaggtcc ggcgcacgaa ggcgacgccc
     2581 gtcgatccgc cgaagctcga cgagctcgag agcacgacga cgccgaccgc gtccgatccg
     2641 ctgcttcgcg aggcgatcat cgactgccgc aacaagctgc cgcccaagcc gaggcaggcg
     2701 ctcgacagcc ggctcgcgag cggcggcgtg cacgacgatg ccgagcttgc cgaagcggtc
     2761 ggcatgcggc tcaacacgtt cctgcagaac ttcacgcgcg cgcgggccct gctcgccgag
     2821 tgcttgaaga agcgcgggat tttcttcgag gggctgacat gacgcgcgaa gaagaagacc
     2881 tcctcgtcga acaggtggcg ggcgcgtatc ggccggtgtc cagcggcgag ctccagtatc
     2941 acaaggcgtg gctcgacctc gacgaggcgg ctcgcgtccg cgcgtacgag ctggcgcgcg
     3001 cgcaacgtcc gctagaagct gcactcgacg ccgacggtct ctcgaccacc gcgcgcgctg
     3061 ttctcgcacg catcactgaa aagatgtgag gcagcggaca gcacgcgcgg cgctagcgcg
     3121 atgcagtctg tgagcagcgt tgcgaacgtc gaagatgtgg acctaactgg cccttgcgac
     3181 cgacatcatc gagcgtgcaa cggtgcctgc atgaagcacg tgctcgtcct cttcgcgctc
     3241 tccgtcgtcg caggatgcgc gaccgagttc acgccgaagc cgtgcgcgct cgacagtgag
     3301 tgtgggaatg gagcggtctg cgagatgcgc ggttcggatc ccgtttgcgt ctatacggac
     3361 gatgcgccga tcgtggtcgg tcagagcgcg ccgatcagcg gcacgaatca agcgctcggc
     3421 accgcgatga agctcggcat cgagatcgcg ttcgacgagc agaacgcggc gggcggcatt
     3481 cgcggccgca agctgcagct gtcgtttcgc gacgacgcct atcagccaaa cctcgccgaa
     3541 gcagcggcgc gctcgctcgt cgatgcgcag gtctcgacga cggagattcc gaagtgcccc
     3601 tcgacgacaa atccgtcgcc cggacaggcc cccgtctcga cgaccgcgat ctcgcgcggg
     3661 cccgatgccg tcatcgcgat gctcggtagc gtcggaacgc cgacaatggt gcgggcggct
     3721 ccggtcgcgg tcgagagcgg cacgatcttc ttcggcgcgt ttaccggcgc gaccacgatt
     3781 ctgcgcgaca tgcaggcggg cccgtgcggc cgctacatct tcaacattcg cgcgagctac
     3841 tcgcaggagg cgcacgcgtc gatggagctg ttcaagaaga agggcgttgc cggctacaag
     3901 aacgtgatca gcttcgacca aaacgactcg ttcggtcagg ccggctacga cggtctcgtc
     3961 gcagcgtaca aggacgtcat cggcaacttc ccgggcagcg ccgacgatac gaacccgatc
     4021 gttcgcttcc gctacacgcg caatgacgac gcgagcgttc cggcgcaagc aaccgcgatg
     4081 cagtcgtatc tcgcgcagct gctcgcgaac cagagcggga cgcagaccgt cggcatcttc
     4141 atgacggaca cctatggcgc cgccgccgag ctgatcaagt cgctgcggac gtggcagtac
     4201 gcgaacgatt cgcagcagca gtcgctccag aaggccacgc gcctcaagct ctacttctcg
     4261 aacgtctcgt tcgtcgggcc gaatgcgctc gcggagcggc tcgtcgctgc gggcaccgtg
     4321 tcgacgccga gcgggccgat gccactcacc gacaacgtga tcatctcgca ggtcgtcccg
     4381 aactatcaga gtgactcgag cgatctgatc gccgcataca accgagccat cgcggcgcgc
     4441 ggcatgaagc ccgacttcac gtcgctcgaa ggctacatct cggcgcgtgt cttcattgca
     4501 ggcctgctcg agcacgaggg gccgttcacg ccagatggct tgatcaagac attcgagaag
     4561 ctgcctgacc tcgcgctcgg catcggcgca tcgtccggct tctcgccgaa tcatcaccag
     4621 tattcctcgt cggtgtgggg cacagcgatc caacccgacg gcacgttcaa gaacctctat
     4681 ttctggagcg acggcagctc catcatgttc ttcgaatgag caacgaacgc gaagccattc
     4741 aggtcggccg atacttgctt catcgcaaga tcgcacgcgg aggtatggcg tcgatccaca
     4801 tcgcacgcct catgggcgag cttggcttct cgcgcatcgt cgccgccaag cgcatgcatc
     4861 ccgagctgat cgaggatcag gagttcgtcg cgatgttcct cgacgaagca cgcgtcgctt
     4921 cgaaggtgca tcaccgcaac gtcgtccccg tgctcgacgt cgtaacactc ggcgacgaag
     4981 tgatgctcgt ccaggagtac atccacggcg cgccgctttc gctgctgctc cagaactcct
     5041 gctcgacgaa cgcgcatgtc ccggtgcccg tcgccgccgc gatcgcgtgt caggtgctcg
     5101 ccggtctgca ttgcgcgcac gagacgacgg acgagctcgg cgctccgctg cagatcatcc
     5161 accgcgatgt gtcgccgcag aacatcatga tcggcaccga cggcagcgcg cgtctgctcg
     5221 acttcggggt cgcgaaggcc gcggcgagcg cgcacgtcac gcgaaagggc acgttcaagg
     5281 gcaagctcgg ctactcggcg cccgagcaaa tccggggtga ggccacgcga cagagcgacg
     5341 tctactcgct cggcgttgtg ctgtgggagc tgctggtcgg caagcgcctg cacgcgaacg
     5401 tcagcggcga ggcccagctc atcgcgcaga tcatggcggg caagtcatcg acgatcaccg
     5461 aggcgctcga agccgaacgc tcgtggatcg gcgcctatcg ctggagccag ctcgaagcga
     5521 tcgagccgat cgtacgcaag gcgctggatc cgatcgcgcg gcgccgctgg cagaccgcag
     5581 cggatatgga agaagcgttg tcagctgcgg tcccggtcgc gagcggcgcc gacatcgcgc
     5641 agtggctcaa agccgtcggc aaagacttcc tcgaagagcg cgagaagatg atcgccgagg
     5701 aagaagcaag ctggcgaagc gccggctcat cgatgcagct acccgcgctc acgaccgtcg
     5761 agattcctgg cgcgatccca acgctcgtgc cgacgagcac cgcgacggag ccgaacgcga
     5821 tgcccgaaac cggcgcgagc ctcatgtgga agcgcggctc gaccgcggtg atcttcgcgc
     5881 tcgtcgcgct gctcggcatt agcgtcgcat tcctcatgcg cgatctcgag cacgcaccgg
     5941 tcgctgcgcc acctccatcg tctccaccgc ccgcgactgc aagcgtgccc acacctccgc
     6001 ccgcgcccga tgcgcctgtc gttcaaaacg tcgtcgagaa aacggtgaag aagccggcgc
     6061 ccgtcgtctc gtcgccacca ccggtaaagc gcgtgactgt ggcgacagcg cccgcacgaa
     6121 tccgcgccgt gcgccctcca cctgcacgct cggcaccacg tacgcccaca gcggctcctt
     6181 caccgcccaa acaacccgct gttacgctgg attgcacgac gcccttctat ttcgaggggc
     6241 gtaagaaggt tttcaaacca ggttgtttat gaccactacg ttgcctgcaa agccccgcac
     6301 gaagagccag acctggggag acgaatctgt cgtctctccg ctcgacgttg gcgcggaacc
     6361 gactgcgcgc gcgccgaaga agtcatccct gctgagctcg cgatccgaag gtgagatggg
     6421 aacgttgatg gtgggcggcc ccgagaagcg cggcatccat gcagcactcg cgcagctgct
     6481 ccacgagcac ggcgtgcaca tcgtccactc gggacagcac accggtggcg gcatgttctt
     6541 cgagcgcatt cgcttcgacc tgtcgcagat ctcgaccggc cgcgagatcc tcgacgcatc
     6601 gatccgcgcg atgtgcgcga agtggggcct cgactatcgg ctctacggcg atcgcaagaa
     6661 gcgcgtcgcg atcctcgtgt cgaagtacga gcactgcctc tacgacctgc tcgtacgcta
     6721 tcgctccggc gatctgccgc cgtgcgaaat ctcgttgatc atctcgaacc atccggatgc
     6781 gaccgactgc gcggagcact tccagatccc gttccgtcac ctgccggtca ccgccgacac
     6841 caagcagcaa caggaacaac aaattatcgg cctcctgcgc gccgctaaca tcgagctgat
     6901 cgtgctcgcg cgttacatgc agatcctgtc gccggcgttc gtcgatcgat atccgcaccg
     6961 catcatcaac gtgcaccact cgtttctgcc cgcgttcatc ggcgcgaacc cgtaccgcca
     7021 ggcgtacgag cgcggcgtga agctcatcgg cgcgacgagc cactacgtca ccagcgacct
     7081 cgaccaaggt ccgatcctcg agcaggaagt gatccgctgc acgcaccgcg acaccgtcga
     7141 ggacctgacg cgcaaggggc gcgacctcga gaagcgcgtt ctcgccgccg ccgtccgcct
     7201 gcatctcgag gaccgcgtga tggtcaacga gaacaagacc gttgtgttcg agtaaggccg
     7261 gtgctgcaat gcggccgtgc gttggctcgc gtgcgcctgg ctcctcgtcg tcggatgttt
     7321 tgatccgccg accggcccgc cgtacaagat aaagctgctg tccgcggatc cgccggtggt
     7381 ctcggttcac ggcggcgagt gggtgacgat cggctacacg ggcgagctgc cggagagcta
     7441 cgccgtcgtg gtcgacgagc tcgttgggac cgtgatcttg gaagatcgcg acgcgcacac
     7501 gttcacgctc agcgtgccgg cacatcccga cggagatgtt ctgatcacgg tggtcaacat
     7561 cgaccgcgat cgcgtggtcg cgagcaacgg cgaattgctc tcgtatcagc gagtggccgg
     7621 catgccgcac ctgaccacgt cgtcgagcac agacgacacg ttgggctggg acctccaccc
     7681 ggagctggcg cgcccatgcg ctcgtgccgg gtacgtgtat ctggagaata tcggagacga
     7741 cccgctcacc ttcgctgccg cacgctcgac caacgccgag ttcacgatcg actcggcgat
     7801 gtgcgacgcg ctcgagtacg gagaggcgtg cgcgatcggc gtgtgcttct cgtcgacggt
     7861 gccgggcttt catgcagcat cgatcattgt cagtacgagc gcaggcgagc ttgcggccag
     7921 cgtgcaaggg aacgtgacgg ctgcgaccgc ggggctcgat ccgacgtttc acggcggcgg
     7981 cgttgtactc agctcgggcg aggagtcgtt ctacggacac gctatccggc gaccggacgg
     8041 aaacggcatc gtgacgtggt cgcgtacgcg cggcatggct ctcgacgtcg caggccatga
     8101 gatcgcgcgt gacctgaccg gtcatattgg tacagcgccg gcggattggg tcattgcgat
     8161 ccgcgctgga gcaccaggcg aaggattcga cgcgctcgtc gcgaacacga acaatttcgc
     8221 gatggcgatt ctcgtgcatc tcgacgattc gtttgcgctc gtgtcgcaga ccgatcttcc
     8281 gttcgactcc gcgaacccct actacgatct tcagcgcgca ccttccggcc ggatactcgc
     8341 gatcggctcg aaagccgtcg tcgcgatagc aaacggtgtc gtggacacga cgtatggaac
     8401 gaatggacgc gtggtgcttc caggcacgta caacaaccgc agcgtcatcg actcccaggg
     8461 acggctctac gctgtcgtgt ccagtagtct catccgcatc accgcgaacg gaacggtgga
     8521 cggatcgttc ggctacggca actcgctgtt ggcgttgacg gtcgactcga ccgatcgcgt
     8581 gttcgtcgtc acgggctcga atgtcgtgcg actctccgaa accggatacg gcgagctgtc
     8641 catcccagcg acgtacgctg acgacatcgc gatcgatgca agtggtcgca tctatctggt
     8701 aggtagcggc cgtgtgtatc gctacagcgg cggcacgcaa gagctgatcg gctacgacac
     8761 gacgtacagc gtcgtttgcc catcgagtgg agcttgctac ctgctcggcg cgaacatgct
     8821 gacggtgcac ccattcacga cgaccgcgcc gagctattac gagccgtacg tgcttcggct
     8881 agcgaactag aagaagaacg aaccgttcac ctggaacgag tcgaagtcgc tcgacacgct
     8941 cttgaggtac gacgtctcga ggaacaggcc cttcttctga ccgacgcgca cgcccatggt
     9001 gccgccgacg agatggttca tgcccgacga cgaagacatg tccgtttcgt cgacgctctt
     9061 ggcgtaacga agcacgtact tcggcgagct gtacacggcg aagaagcgcg acacatcgta
     9121 gctggcataa acaggcacga tcgcatcgac gatcgtcgac gaggtctgca tgccttccgc
     9181 gcccgacgag atcttgagat agccagtacc gataccggcc gcgaccgcga gcttgcccgc
     9241 gttgaggaac tggtacttcg cgtcgacgcc cgcggtgccc ggcagcgtga ccttcgcacc
     9301 gacctcgatc ttgtcgacga tgccgcggcg atagccgagc tccatgtacg gcatcttcag
     9361 gctgacgtcg tcgccggtcg cttcggaggc gtcggcatcg agggacgggc tcgcgtagta
     9421 accaccgccg acgaggatgc gctggctgcc cgggtcgagg gcctttgcgg tctggagggt
     9481 agagctgttc acacaacccg cggaagctgc agcaattagg agaagagagg cggcggcgaa
     9541 tttcatgttg agtggtcctt tcgaggtggt aagacgagga gccgtcgccg tctgggaact
     9601 tttttgtatg cgcttgtggg tgtcactgct ggttcttgtc gctgtggcgc attcagcgca
     9661 cgccgagcag cgctatgcag ttctcgtcgg cgcgaatccc ggttggtcgt cggatcggcc
     9721 gctgcgctac gcagaagatg atgcggaccg cgtgcgcgat gtcctcgtca cgctcggcgg
     9781 cttctcagcc gatcacgtct cgctgcttcg cgatcccgat acgaatgaag tgcgcgccac
     9841 gctgcgcgat ctcgttcgca gcacgcaggg ctcgagcgaa gccacgatgg tcttcttcta
     9901 ttactcgggc cacgcggata acgagcgcgt tcaccttcgt ggcgacgtgc tgacgttcaa
     9961 ggagattcgc gacacgctgc gcgcgctgcc gtcgacgatc aagctcgcgg tgatcgacgc
    10021 atgcaagtcc ggcgcggtcg tgcgcaaggg cggctcgcgc atcgacgact tcgaagtgtc
    10081 ggtcgataac ccgaagctct cgggcatggt gctgctcacg agcagcggcg ccgacgagct
    10141 ctcgcaggaa tctcgcgcgc tcgcgggctc ggtgttcacg catcacctcg tgtcgggctt
    10201 gcgcggcgcg gccgatgcga acggcgacaa gcaggtgacg gtggccgagg cctatcatta
    10261 tgcatatgcg cgaacgcgcg ccgacaccgc gtcgacgggc acgccgcagc ggccttcgtt
    10321 tcagtacgag ctgtcgggac aaggcgagct cgtgctctcg cagctcaaga ccgcgaagac
    10381 ggcgaagatc acgctaccga agggcaacgg cgacaagtat gtggttctcg acagccacga
    10441 gatgcgactc gtcgcggaag cgcgcagcga gaaggatcac gaagtcgagc tgacgctcgc
    10501 tcccggctcg tatcacgtga agaaggtgct cagcgacaag ctcgaggtcg cagcgatcgt
    10561 cctcgcggcg ggcgaagcgg cgagcacgag caagattgca tacaaggatg cgccgctcgc
    10621 gcagggcatc gtgaagggca gcgccgagga cctctcgccc gacgagcagc gcgagtggca
    10681 gcgatcgcaa gcgttcggcc tgctcgccgc gggtaacccg aacgccgcga tcacggtctt
    10741 cgacacgctg ctgcgcgcca accccggcga tctgctgtcg tggcgcggcc gtgcgcgtgc
    10801 gctgatccgg atcgcggaga cgtatggggc cgtcaacgac taccagcgcg agaagctcgc
    10861 gatgtcggaa gcgctcaagg cggacccgct gctcacgcag gatccgagct tcaagtcccg
    10921 ctacgagaag cttgccgagc tcgacgcgaa ggatcaggcg acgctcaacg cgaggctcgc
    10981 ggccgagcaa gcgatccgca agaacccgcg cagcgtgaag cacgtgggct tcggcttcga
    11041 tctactgagc gcgcgcggca tgttcacggt cgatgcctcg ctggtcgtca agcgcatgat
    11101 cttcccgcgc gtcgcgatcg atgtcgggag ccagggcttc gatgcgggcg tcgtgatcgc
    11161 gccatatccg tcgcgctgga gcccgttcct cggcattggt ggtcacgtgt cttggcgcaa
    11221 gatgggcatc ccggtcggtg cgcactccga catgatcaac gtcaacgaca acatgtactc
    11281 gacggaagaa atgtggggac tccacgggcg cgccgaagcc ggagcgcagt acgtcgggcg
    11341 atcgggattc agtaccgagc taggactcgc tctgatcgca ttccgagagg cgggaggtag
    11401 gactgtccag caagcctggc caatcttcca cttcgggtgg gtttggtaga aaagctaccc
    11461 aacttccgag cgccgttcgt ctagcatggc atggaggatg gctccgcaag ggcaaggcga
    11521 tgatctcgcc gagctgtata agcgccacgc tcctgcgctc catcgccggt gtgcgagcat
    11581 cgtcggcaac cccgaggacg cccgcgacct ggtgcaagaa accttcgcgc gctacatcac
    11641 cgcgcgctcc acattcaccg acaaagcgtc gccgttcacc atgctgtacc ggatcgccac
    11701 caacgcatcg atcgatcggc tgcgccgccg caagacggcc accgaggaag agctcgatcc
    11761 agaggtgcgt gccggtgacc tcggacgcga gccgcaccgc gtcgacaaac tgcatgatct
    11821 cgcgttactc accaaaggcc tcaccgagga ggagctgacc gtggcggtgc tccatcatct
    11881 cgacggctac acgcaggacg gcatcgcggc gtccctcgac atctcgcgca agacggtcgg
    11941 caagatcctc gcgaagttcg aggaccacgt gaagaagcgg gccgcgcggc tcggcttcga
    12001 cgatcggagg gcaagtggca cctgaagagc gtgtccgcaa gctcaccgat ctagagctcg
    12061 agcgcgagct tgcgggcgat ctccccgctg cacgtatggc cgaagcgacc gacgcagatc
    12121 gcgcgcgctt gggcgagctc cgcgccgagt cggaagcgtt cctgcgctcg gttgacgtcg
    12181 acatggaggt caagcgcatc cagcagcgcg tcgagcgtgc aaagccggag cgcgcacctg
    12241 cgtggtggcg attcctgatt cccgccggcg cactcgccgc tgcggccgcc gtgctgctcg
    12301 tgttcctccg tcgaggagac tcgccgaacg gtcccggcga cgacatccag ctcaaaggcg
    12361 acggtgtcgc gctgatcatc cacctcgcga cgagcgggca gagccagcag ctcggtgatg
    12421 gcgacaacgt gaagccgggt ga
//
LOCUS       DATLBZ010000154         4952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4611627, whole genome shotgun sequence.
ACCESSION   DATLBZ010000154 DATLBZ010000000
VERSION     DATLBZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4611627"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VMZ53_08190"
     CDS             complement(<1..758)
                     /locus_tag="VMZ53_08190"
                     /inference="COORDINATES: protein motif:HMM:NF015716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HUS28473.1"
                     /translation="MIEPMPQDSRPAALLRGTARGLEIVIDATAPTSAITGAVTKRLD
                     EAPGFFRGSDVRIRVEDGPLPGGCLARLDDLSLQYELRIVEVTAQKRAKTEADAVPVP
                     EQLAAGSAPNEFEEESPTNAGAKLISLPPASAEMLEPAALSDADMLEEICDAEPAAMT
                     ATIVEGTPETFDEPTQTAIAAPLTLASLPDAELATRTPGTRIVIGPVRSGVILDHTGH
                     VVVFGDVNPGAEVRAAGNIVVLGRLRGTAHAGIG"
     gene            886..3228
                     /locus_tag="VMZ53_08195"
     CDS             886..3228
                     /locus_tag="VMZ53_08195"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HUS28474.1"
                     /translation="MTRKTLFALVLAAACSPKAPAPSETGPQDRSTAATAGGQTSQTA
                     APLPAPDPREKALATTVVELFEQEHLLRKKIDDGISKEAFKNYLETLDPGKMFLLASD
                     RDALSKYADKIDDELHAGSLELAHEGQKAFTGRIEVVQKFVDELLAKPFDLTNEESVE
                     LDSEKLELAKSDDELRERWRQRLELEVLERIASMDARLKADAEKKAGKKDDKKDDKNA
                     KKDDKKLPQDTPAGDDPGADDDEDKSAPPVAQIPKTPEGREAKAREDLAKTYSSRFAR
                     MKNPGQLDAASDLINAVASTLDPHTTYMPPADKANFDIRMSGSLEGIGAVLREKDHLI
                     EIVEIVPGGAAYRQGGLVVGDLIESVQQEGKDPVDVFDMRIDDVVSMIRGAKGTIVRL
                     RVQKASGQEQTIAITRDVVVIEESYAKGAILQPKGGKATYGYIHLPSFYGGKGSPRSA
                     DDDLKKLLEDMRAKKVSGVILDIRSNGGGLLTGSINITDHLIDKGPVVQVKDSDGDKE
                     VLPARTKGESYDGPMIVLVDRFSASASEILAGALQDYGRAVIVGAGGTHGKGSVQTLA
                     DLDRATNGQIELGVLKITIQQFFRPDGDSVQLQGVTPDIMLPDPTAYIDSGEGELKHA
                     IPASKIEAAPFAKWPVQFNVKDLAAASSARVAKHPLLAKIASATAVLRSRKNDTKVPL
                     QKTAWEKRRDEQKAQLDAASPDLKKVAPVFTVKPMEDSPAPATAPAPGGSAGAKDDKA
                     AKWRDNLARDPWVEETLNIMNDMTQQKVAAGSAGPGPGKK"
     gene            3325..>4952
                     /locus_tag="VMZ53_08200"
     CDS             3325..>4952
                     /locus_tag="VMZ53_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28475.1"
                     /translation="MRVLPVATVLFCAACGSSVSPTEAQKVSFERWSAPQATGHDILL
                     GSRGDVVVMAHRYSLDAGATWKPLDAKLGEPTRVSLVGNTVALYANGLVRWSPADGSI
                     TTVAGAPSYASDRTWRVDPTNARFIAFDAVENALAVEQTSGWITAKLPQPSTTEVRPY
                     IRDIESNGSVLVSTSVWGVHRSTDGGASWQLVTASTPNAGRDLLVLHDGSFALVGGTT
                     TYAFDATGQATGTLANLVLSTEATVCEDGTIVTGTKLTRDLGATWQTLIAQSDLQMSV
                     QRASCGGAGTYWVLVISDAWGYRLVRFDGVGSPGRAAGNWEAVGDQAWTSGGPPIVRT
                     DDGTFLVAGLALAPSATDWTLQEIPAKTWAAGTTLFGVAKQKFFASYDGGVTWSAAAA
                     DGLMASDPEAFAKSPDGALYVAQFEGGTVDGQDLWRSSVWKTQDQGATWTMAYDAKAM
                     RVHGADEVTGEVHRFVGITMAGDWIATDAVSRDGGSTWQKTTVLGDRGLAHLTRQGSL
                     VTGGADEKVWRVYDDGGLGELRATYQIVVEGNPVP"
BASE COUNT          932 a         1600 c         1702 g          718 t
ORIGIN      
        1 tggccgatgc cggcatgcgc ggtgccacgc aggcgaccga gcacgacgat gttgccggcg
       61 gcgcggacct cggcgcctgg gttgacgtcg ccgaacacga cgacgtggcc ggtgtgatcg
      121 aggatcacgc ccgagcggac cgggccgatc acgatgcgtg tgccgggcgt ccgggtcgcg
      181 agctcggcgt cgggaagcga cgcgagcgtg agcggtgcgg cgatcgcggt ctgggtcggc
      241 tcgtcgaacg tttcgggggt gccctcgacg atcgtggcgg tcatcgcagc aggctcggcg
      301 tcgcaaatct cctcgagcat gtcggcgtcg ctcagagcgg ctggctcgag catctcggcg
      361 gaggcgggcg gcaacgagat cagcttggcg ccggcgttcg tgggcgactc ttcctcgaac
      421 tcgttcggcg ccgacccggc ggccagctgc tcgggaactg gaaccgcatc ggcttcggtc
      481 ttcgcccgct tctgggcggt cacctcgacg atgcgcagct cgtactgcag ggacagatcg
      541 tccaggcggg cgaggcagcc acccggcagg ggtccgtcct cgacgcgaat gcgcacatcg
      601 ctgccacgga aaaatccggg ggcttcgtcc agccgctttg tcacggcacc cgtgatcgcg
      661 gaggtgggag ccgtggcatc gataacgatt tcaaggcccc gagccgtccc ccgcaggagc
      721 gccgcaggac ggctgtcctg gggcatgggc tcgatcacgt cctgaccggc gatagatgga
      781 tccataagtg cccttcaaaa cccgtaatcc gaccgcacga agtgggcaac ttttcaacat
      841 cccatcgcag gtggaaacgg cccgatcacc ggctacattg aagaggtgac gcgcaaaacc
      901 ttgttcgcgc tcgtccttgc ggcagcctgc tctccgaagg cccccgcccc tagcgagacc
      961 ggtcctcagg acaggtccac ggcagcaacg gccggcggcc agacctcgca gaccgctgct
     1021 ccgctgcccg caccggaccc gcgcgagaag gcactggcca ccaccgtggt cgagctgttc
     1081 gagcaggagc acctgctgcg caagaagatc gatgacggga tctcgaagga ggcgttcaag
     1141 aactatctcg agacgctcga tcccggaaag atgttcttgc tcgcgagcga tcgcgacgcg
     1201 ctcagcaagt acgccgacaa gatcgatgac gagctgcacg ccggctcact cgagctcgcg
     1261 cacgaaggcc agaaggcatt caccggacgc atcgaggtcg ttcagaagtt tgtcgacgag
     1321 ctgctcgcga agccgttcga tctgacgaat gaagaatcgg tcgagctcga cagcgagaag
     1381 ctggagctcg cgaagagcga cgacgagctg agagagcgct ggcgccaacg cctagagctc
     1441 gaagtgctcg agcggatcgc gtcgatggat gcgcgcctca aggccgacgc cgagaagaag
     1501 gccggcaaga aggacgacaa gaaggacgac aagaacgcga agaaggacga caagaagctg
     1561 ccgcaggaca cgccggccgg ggatgacccg ggtgccgacg acgacgaaga caagagcgcg
     1621 cctcccgtcg cgcagatccc caagacgccc gagggccgcg aggccaaggc ccgcgaggat
     1681 ctcgcgaaga cgtactcgag ccgcttcgcg cgtatgaaga atccgggcca gctcgacgct
     1741 gcgtccgatc tcatcaacgc cgttgcatcg acgctcgacc cgcacacgac gtacatgccg
     1801 ccggctgaca aagccaactt cgacatccgc atgagcggct cgctcgaagg catcggcgcc
     1861 gtgctgcgcg agaaggatca cctcatcgag atcgtcgaga tcgtgcccgg cggtgcggcg
     1921 tatcgccaag gcggcctcgt cgtcggcgat ctgatcgaga gcgtgcagca agagggcaag
     1981 gatccggtcg acgtcttcga catgcgcatc gacgatgtcg tctcgatgat tcgcggcgcg
     2041 aagggcacca tcgtgcgcct ccgcgtgcag aaggcgagcg gccaagagca gacgatcgcg
     2101 atcacgcgcg acgtcgtcgt gatcgaggag tcgtacgcga agggcgcgat cctccagccg
     2161 aagggtggca aggccacgta cggctacatt cacttgccga gcttctacgg cggcaagggc
     2221 agcccgcgct ccgccgacga cgatctcaag aagctgctcg aggacatgcg cgcgaagaaa
     2281 gtctcgggcg tcatcctcga cattcgcagc aacggtggcg gcctgctgac cgggtcgatc
     2341 aacatcaccg atcacttgat cgacaagggc cccgtcgtcc aggtgaagga cagcgacggc
     2401 gacaaggaag tgttgcccgc gcgcacgaag ggcgagtcgt acgacggccc gatgatcgtg
     2461 ctcgtcgatc gcttcagtgc atccgcatcg gagattctgg ccggcgcgct tcaggattac
     2521 ggccgcgcgg tgatcgtcgg cgcgggcggc acgcacggca aaggcagcgt gcagacgctt
     2581 gctgatctcg accgcgcgac gaacgggcag atcgagctcg gtgttctcaa gatcacgatc
     2641 cagcagttct tccgccccga cggcgactcg gtgcagctgc agggcgtgac gcccgacatc
     2701 atgctgcccg atccgacggc gtacatcgat tcgggcgaag gcgagctcaa gcacgccatc
     2761 ccagcgtcga agatcgaagc agcgccgttc gcgaagtggc cggtgcagtt caacgtgaag
     2821 gacctcgctg cggcgagctc tgcgcgcgtc gccaagcatc cgctgctcgc aaagatcgcg
     2881 tcggcgacgg ccgtcctgcg ctcgcgcaag aacgacacga aagttccgct ccagaagacc
     2941 gcgtgggaga agcgccgcga tgagcagaag gcgcagctcg atgcagcctc gccggatctc
     3001 aagaaggtcg cgccggtctt cacggtgaag ccgatggagg attcgccggc tccggccacg
     3061 gcgcctgcac ctggcggctc ggccggggcg aaggacgaca aggccgcgaa gtggcgcgac
     3121 aacctcgccc gcgacccgtg ggtcgaggag acgctcaaca tcatgaacga catgacgcag
     3181 cagaaggtcg ccgcaggcag cgccggcccc ggccccggca agaagtagcg aacccgcgct
     3241 ctccggcaca ccgcgcagga gcgcactggt gagggacgcg tcagacgggc aagcgcctga
     3301 gaggcgtagc gcccatcctt ctgcatgcgc gtcctccccg tcgctacagt cttgttctgc
     3361 gccgcgtgcg gcagcagtgt ctcgccgact gaagcgcaaa aagtatcgtt cgagcgctgg
     3421 tcagcgccgc aggcaacggg ccacgacatt ctgctcggct cgcgcggcga cgtcgtcgtg
     3481 atggcgcatc gctactcgct cgatgcaggc gcgacatgga agccgctcga tgcaaagctc
     3541 ggcgagccta cgcgcgtctc gctcgtcggc aacacggtcg cgctctacgc gaatggcctc
     3601 gtgcgctgga gccctgcgga tggatcgatc acgacggtgg caggcgcgcc gagctacgcg
     3661 agcgaccgca cgtggcgcgt cgatccgacg aacgcgaggt tcatcgcctt cgatgccgtc
     3721 gagaacgcgc tcgcggtcga gcagacgagc ggctggatca ccgcgaagct gccgcagccg
     3781 tcgacgacgg aagttcggcc ctacattcgc gacatcgaga gcaacggcag cgtgctcgtg
     3841 tcgacgagcg tatggggcgt gcatcgctcg acggacggcg gcgcgagttg gcagctcgtc
     3901 actgcgtcga cgccgaatgc tggccgcgac ctgctcgtct tgcacgacgg cagcttcgcg
     3961 ctcgtcggcg gcaccacgac gtatgcgttc gacgcgaccg gacaagcgac cggcacgctc
     4021 gcgaatctcg tgctctcgac ggaagcgacg gtctgcgaag acggcacgat cgtcaccggc
     4081 acgaagctga cgcgtgatct gggcgcgacg tggcagacgt tgatcgcgca gagcgatctg
     4141 cagatgagcg tgcagcgcgc gagctgcggc ggcgccggca cgtactgggt cctcgtgatc
     4201 agcgacgcgt ggggctatcg cctcgtgcgc ttcgacggtg tcggttcgcc gggacgcgcg
     4261 gccggcaact gggaagcggt cggcgatcaa gcgtggacca gcggcggccc accgatcgtt
     4321 cgcaccgatg acggcacgtt cctcgtcgcg ggcctcgcgc tcgcacccag cgcgaccgat
     4381 tggacgctgc aggagattcc ggcgaagacg tgggcggcag gcacgacgct gttcggtgtt
     4441 gcgaagcaga agttcttcgc gagctacgat ggtggcgtca cgtggtccgc cgccgcagcc
     4501 gatggcttga tggcctccga tcccgaagcg ttcgcgaaga gccccgacgg tgcgctctac
     4561 gtcgcgcagt tcgaaggcgg cacggtcgac ggtcaggacc tctggcgcag cagcgtgtgg
     4621 aagacgcagg accaaggtgc gacctggacg atggcgtacg acgccaaagc gatgcgcgtg
     4681 catggcgccg atgaagtaac cggcgaagtg catcgcttcg tcggcatcac gatggccggc
     4741 gattggatcg cgaccgacgc ggtctcgcgt gacggcggca gcacctggca aaagacgacg
     4801 gtcctcggtg accgcggcct cgcgcacctg acgcggcaag gcagcctcgt gaccggcggc
     4861 gccgacgaga aggtgtggcg cgtctacgac gacggcggcc tcggcgagct ccgcgcgacg
     4921 taccagatcg tcgtcgaagg aaatcccgtt cc
//
LOCUS       DATLBZ010000155         4693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3872020, whole genome shotgun sequence.
ACCESSION   DATLBZ010000155 DATLBZ010000000
VERSION     DATLBZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3872020"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VMZ53_08205"
     CDS             complement(<1..446)
                     /locus_tag="VMZ53_08205"
                     /inference="COORDINATES: protein motif:HMM:NF036808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28476.1"
                     /translation="MKARRYQVRKEAASIIEGGHPWIFREQLSSAASIFADGDWLRLV
                     DGQNRVIGHGIFEAEGAIAIRVLRTGAEPPDAAWLRATLRTALSRRTSLAAAATTGIR
                     LVHGESDAIPAVVVDRFGDTLVVSSYSSGADALARYIAAALVAEARG"
     gene            complement(470..2452)
                     /locus_tag="VMZ53_08210"
     CDS             complement(470..2452)
                     /locus_tag="VMZ53_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HUS28477.1"
                     /translation="MKIACIGGGPAGLYLGILVKRSAPEHEVVVYERNRPADTFGFGV
                     VFSDATLGHLAAADPETHAQITAEFARWDDIEIHVGGEVLRSTGHGFCGLERKTLLQI
                     LQARAQALGVRIEFEHEVKSLDELRDADVIVACDGVASWVRDKLAAEIQPRVDVRPNK
                     FVWLGCTVPYDAFTFLFKQTQFGLFRVHAYRYQETGSTFIVECREDTWRAAGLATADE
                     DQTVAILSDIFATELAGHKLIKNRSIWRNFPTVRCGHWHTGNVVLVGDAVHTAHFSIG
                     SGTKLAMEDVIVLRDELVRIRERSQVPAALAAYEAKRRPEVEALQAAAQASLEWFEGT
                     ERYMTMAPVQFTYSLMTRSLRVSHASVAKRDPHLARGVEQLLAAGVGVHAMPATGTAG
                     DSGLLPIASLGGGMPALPALPPPTALPCPLGDRRAANRIVLAPPPLPKTARESIAGDD
                     ELVALGAAASTGAGLVVTPPISVVGPTMPRINTDDQAVAWRRVVDYVHGKNALIVARI
                     STAGAVPGALEQAIQRAAFSGFDMLLLDPGHDGPAIEYLPALVEIARQTWRKGGYIAA
                     CVHDRPTTRAAVVGHAAQLARAGADMLWVTSAGDITMTGARLPAAPLADRLRNELDIA
                     TAVDGSDAVLPDLDAAIAAGRADLVVVERMPAGARR"
     gene            2492..3043
                     /locus_tag="VMZ53_08215"
     CDS             2492..3043
                     /locus_tag="VMZ53_08215"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS28478.1"
                     /translation="MKHRRHRRVEAKGVAAHIDAEAADDDFAIENISVGGLFIRTSTA
                     MPIGMPVRVDLLKPGSKTGGVLSLQGRVVSVVSEREAAKQDIAPGVGIEFDAMSIELE
                     KRLHALLRDLGLKDLAEPTAIDPDSLYATASPDTQQVAANVRGLLEMLTEALQKVKER
                     DDEITKLKAEVRRLTTELRTVKK"
     gene            3045..3695
                     /locus_tag="VMZ53_08220"
     CDS             3045..3695
                     /locus_tag="VMZ53_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUS28479.1"
                     /translation="MLSNARGLVFDLDGTLVDSLGDIMLHLNAALAEKGLPGWSREEV
                     GEWVGYGAENLIIRAVPHPEMVADTLARFREHYRARPVVQTCLYPDLAGVLDAISQGR
                     KLAVLSNKPHELTVRVCEQLLGAWPFAVIAGQRSDKPHKPDPAALLDVCNELALPPSD
                     CVMIGDSEVDVQTGRAAGVPSVAVSWGLRARSIIEAAGPSYLVDTPAQLAALFTGR"
     gene            complement(3682..4275)
                     /locus_tag="VMZ53_08225"
     CDS             complement(3682..4275)
                     /locus_tag="VMZ53_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28480.1"
                     /translation="MRSALLLAFALAGGCSMYDTDYGPTPFLCGPADQDPRCPEGYAC
                     MLDPGSGSEVCVENGGSISQDFDCADDSAKEPDDLLAMATVTQLDAMRTETIDGRSVC
                     PAGDKDTYAIDIRTTNEDLSITVTMDQNGAKLRAALLNTGGVPISVAMPINGDATMIS
                     AEAMNLPVGMYYAQVYGPAGGDLQVNNYKITLTVSGP"
     gene            complement(4275..>4693)
                     /locus_tag="VMZ53_08230"
     CDS             complement(4275..>4693)
                     /locus_tag="VMZ53_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28481.1"
                     /translation="IEQVPEQGPLRTDFGFGGGGQVDEPARPYQTAKWIATGTAGAAL
                     AFSFVSILEARHEHELLRADSTMCGTPPCRQFDLAYDHRVQTLGQRWDALYKVSLGVG
                     VAASAVAGYFWYRNRSFRVVPAADSSSVGAVAVGRF"
BASE COUNT          780 a         1499 c         1651 g          763 t
ORIGIN      
        1 ccgcgcgctt cggcgacaag cgcggctgcg atgtagcgcg cgagcgcgtc ggcgcccgac
       61 gagtaactcg agacgacgag cgtatcgccg aatcgatcga cgaccaccgc cgggatggca
      121 tcgctctcgc cgtgcaccaa gcgaataccg gtcgtcgcag cagccgctag cgacgtgcgt
      181 cttgaaagcg ccgtgcgcag tgtggcgcgc agccatgcgg cgtcgggagg ttcggcgcct
      241 gtgcgcagca cgcggatggc gatcgcgccc tcggcttcga agatgccgtg accgatgacg
      301 cggttttggc cgtcgacgag gcgcagccag tcgccgtcgg cgaaaatcga tgcggcggaa
      361 gagagttgct cgcggaagat ccacgggtga ccgccctcga tgatcgatgc ggcctctttg
      421 cgaacctgat agcggcgcgc cttcatcgcg gggggctcgt catcacgcgt tagcggcggg
      481 cgcccgcggg catccgctcg acgacgacga ggtcggcgcg ccctgcggcg attgctgcat
      541 cgagatcggg gagcactgcg tcgctgccgt cgaccgcggt tgcgatgtcg agctcgttgc
      601 ggaggcgatc ggcgagtgga gcggcgggga ggcgcgcgcc tgtcatcgtg atgtcgcccg
      661 cggacgtgac ccagagcatg tcggcgccgg cacgggcgag ctgcgcggcg tggccgacga
      721 cggccgcgcg cgttgtcggg cgatcgtgga cgcatgcggc gatgtagccg cccttgcgcc
      781 aggtttggcg cgcgatctcc acgagcgcag gcagatattc gatcgccgga ccgtcgtggc
      841 ctgggtcgag gagcagcatg tcgaagccgg agaaggcggc gcgctgaatc gcttgctcga
      901 gtgcgcccgg gacggcgccg gcggtcgaga tccgcgcgac gatgagcgcg ttcttgccgt
      961 ggacgtaatc gacgacgcgg cgccacgcga cagcttggtc gtcggtgttg atgcgcggca
     1021 tcgtcggacc gacgacggag atgggcggcg tgacgacgag gcctgcgccg gtagatgccg
     1081 cggcaccgag agcgacgagc tcgtcatcgc cggcgatcga ctcgcgcgcg gtcttcggca
     1141 gcggcggtgg tgcgagcacg atgcggtttg cggcgcgacg atcgccgagc gggcagggca
     1201 gggccgtcgg tggagggagc gcgggcagcg cgggcattcc gccaccgagc gacgcgatgg
     1261 gcaggaggcc ggagtcgccg gccgtgcctg tcgcgggcat cgcgtggacg ccgacgccag
     1321 ccgcgagcaa ctgctcgaca ccgcgcgcga gatgcggatc gcgcttggcg acactcgcgt
     1381 gcgagacgcg gagactgcgc gtcatcagcg agtacgtgaa ttggacaggc gccatcgtca
     1441 tgtagcgctc ggtgccttcg aaccactcga gactcgcttg cgccgcggcc tgcagcgctt
     1501 cgacttccgg gcggcgcttc gcttcgtagg cggcgagggc ggccgggacc tgcgagcgct
     1561 cgcggatccg cacgagctcg tcgcgcagaa cgatgacgtc ctccatcgcg agcttcgtgc
     1621 cggaaccgat cgagaagtgc gcggtgtgga cggcatcgcc gacgagcacg acgttgcccg
     1681 tgtgccagtg accgcagcgc accgtcggga agttgcgcca aatcgagcga ttcttgatca
     1741 gcttgtggcc ggcgagctcc gtcgcgaaga tgtcgctgag gatcgcgacg gtttgatctt
     1801 cgtcggcggt cgcgaggcct gcagcgcgcc aggtgtcctc gcgacattcg acgatgaacg
     1861 tcgaccccgt ctcttgatag cgatacgcgt ggacacggaa caggccgaac tgcgtctgct
     1921 tgaacaggaa tgtgaacgcg tcgtagggga ccgtgcagcc gagccacacg aacttgttcg
     1981 ggcgcacgtc gacgcgcggc tggatctcgg cggcgagctt gtcgcggacc catgatgcga
     2041 cgccgtcgca cgcgacgatg acgtctgcgt ctcgcagctc gtccagcgac ttcacctcgt
     2101 gctcgaactc gatgcgaacg ccgagtgctt gggcgcgcgc ttggaggatc tggagcagag
     2161 tcttgcgctc gagaccgcag aagccgtggc ccgtcgagcg cagcacttca ccaccgacgt
     2221 gaatctcgat gtcgtcccaa cgcgcgaact ccgcggtgat ctgcgcgtgg gtttcgggat
     2281 cggcggctgc gagatgaccg agcgtcgcgt cgctgaagac gacgccgaag ccgaacgtgt
     2341 ctgccgggcg gttgcgctcg tagacgacca cctcgtgctc gggcgcgctg cgcttgacga
     2401 ggatgccgag gtagagcccg gctgggccgc cgccgatgca ggcgatcttc acgtcgcgag
     2461 actatcagag tcagcatgat accgtggagg tgtgaagcat cggcggcacc ggagggtcga
     2521 agcgaagggc gtcgctgcgc acatcgatgc cgaggcggcg gacgatgatt tcgcgatcga
     2581 gaacatctcg gtcggtggcc tcttcattcg cacgagcacc gcgatgccga tcggcatgcc
     2641 ggtacgcgtc gatctgttga agcccggctc gaagaccggc ggcgtgctct cactacaagg
     2701 ccgcgtggtc agcgtggtga gtgagcgcga agccgcgaag caagacatcg cgccgggcgt
     2761 cggcatcgag ttcgacgcca tgtcgatcga gctcgagaag cgcctgcacg cgctgttgcg
     2821 agacctcggc ctcaaggatc ttgcggagcc gactgcgatc gatccagaca gcctgtacgc
     2881 gaccgcgagt cccgatacgc agcaagttgc ggcgaacgtg cgcggcctcc tcgagatgct
     2941 caccgaggcg cttcagaagg tgaaggagcg tgatgacgag atcacgaagc tgaaggcgga
     3001 agtccgccgg ttgacgaccg agctgcgaac cgtcaagaag tagtgtgctc agcaacgcgc
     3061 gcggtcttgt cttcgacctc gacggcactc tcgtcgacag cctcggcgac atcatgctgc
     3121 acctcaacgc cgcgctcgcc gagaagggct tgcccggttg gtcgcgcgaa gaagtgggcg
     3181 agtgggtcgg ctacggggcg gagaacctca tcattcgcgc ggtgccgcat ccggagatgg
     3241 tcgccgacac gctcgcacgc ttccgcgagc actaccgcgc gcgtccggtc gtccagacgt
     3301 gtctctatcc cgatctcgcg ggcgtgctcg atgcgatatc gcaggggcgt aagctcgccg
     3361 tgctgtcgaa caagccgcac gagctgaccg tgcgcgtctg cgagcagctg ctcggcgcgt
     3421 ggccattcgc ggtgatcgcg gggcagcgca gcgacaagcc gcacaagccg gatcccgcgg
     3481 cgctcctcga cgtgtgcaac gagctcgcgc ttccaccgag cgactgcgtg atgatcggtg
     3541 actctgaagt cgacgtccaa acaggacgcg ccgctggagt gccgagcgtg gccgtgagct
     3601 ggggactgcg cgcgcgatcg atcatcgagg cggccggccc gagctatctc gtggacacac
     3661 cggcccagct tgccgcgcta tttacgggcc gctgacggtc agcgtgatct tgtagttgtt
     3721 cacctgaaga tcgccgccgg ccgggccgta cacctgcgcg tagtacatgc cgacagggag
     3781 gttcatcgcc tcggcgctga tcatggtcgc gtcgccgttg atcggcatcg ctacagaaat
     3841 cggcacgccg cccgtgttga gcagcgcagc gcggagcttc gcgccgttct gatccatcgt
     3901 gacggtgatc gacaggtctt cgttcgtcgt gcggatgtcg atcgcgtacg tgtccttatc
     3961 gccagcggga cacaccgagc ggccatcgat cgtttccgtc cgcattgcat cgagctgcgt
     4021 cacggttgcc atcgcgagca gatcgtcggg ctccttcgcg ctgtcatccg cgcagtcgaa
     4081 gtcttgcgag atcgagccgc cgttctcgac gcagacctcg ctgccgctcc ctggatcgag
     4141 catgcaggcg tagccttcgg gacaccgcgg atcctgatcc gccggcccgc acagaaacgg
     4201 cgtcgggccg tagtcggtgt cgtacatgct gcagccacca gcgagggcaa aagcgaggag
     4261 gagggcgctc cgcatcagaa cctccccacg gcaactgcac cgacgctgct cgagtccgca
     4321 gctggcacca ctctgaacga gcgattgcga taccagaagt agccagccac tgcgctcgct
     4381 gcaacgccga cgccgagcga caccttgtaa agcgcatccc agcgttgacc gagcgtctgc
     4441 acgcggtggt cgtacgcgag atcgaattga cgacacggcg gcgtgccgca catcgtcgag
     4501 tctgcgcgca gaagctcgtg ctcgtgtcgc gcttcgagga tcgagacgaa cgaaaatgcg
     4561 agcgctgcgc ccgccgtgcc cgtcgcgatc cacttcgccg tttgatacgg gcgcgctggt
     4621 tcatcgacct ggccaccgcc gccaaagcca aagtcggtgc gcagcggtcc ttgctccggc
     4681 acctgctcga tcg
//
LOCUS       DATLBZ010000156        17250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6036275, whole genome shotgun sequence.
ACCESSION   DATLBZ010000156 DATLBZ010000000
VERSION     DATLBZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17250
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6036275"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VMZ53_08235"
     CDS             complement(<1..564)
                     /locus_tag="VMZ53_08235"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HUS28482.1"
                     /translation="MLTRYSDARLPTPHGEFRVVVYRAGRPGGLGQTAVGLADEEHVA
                     MVMGEVAGPGVLCRVHSSCFTSEVLGSLRCDCRAQLDAALARIALEGRGVVVYLVQEG
                     RGIGLGNKVRAYQLQDEGKDTVEANLALGFGADDRSYEIAVGILRDLGVTSVRLMTNN
                     PAKRAGLEQAGVSVEGLEAHWVGASEHN"
     gene            640..1173
                     /gene="def"
                     /locus_tag="VMZ53_08240"
     CDS             640..1173
                     /gene="def"
                     /locus_tag="VMZ53_08240"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HUS28483.1"
                     /translation="MAVRPTVIWPDPRLREPTIAISTIDESVRELYRDLCDSMYAENG
                     LGMAAPQIGDNRKMFIVEPKLAGMAETDQPVCLMNAEVIETSEETQDSEEGCLSFPDI
                     YVKVKRPMRCKVRYLGLDGQLHEIDGEALMARCLLHENDHLTGKLLVDFVGPLKRQMI
                     KKKLQRVKDAEADEAAG"
     gene            complement(1174..1743)
                     /locus_tag="VMZ53_08245"
     CDS             complement(1174..1743)
                     /locus_tag="VMZ53_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28484.1"
                     /translation="MRVAWLILLAGCYRSTPGATPLANKTPDAAPAALTMTPKGVGPI
                     DNTTKVTLTNLRALLIGYEVRPLNDTSLEYDIYRDGEKLAYVVPDDQSGYVFNVHATS
                     SRVVVSGHPWRVGRAFGDSAHLTNCECWGPNPTCYASGEHIAVNFKRDCEDLTGSDRS
                     ALRALDGEIVQRVIWSPTPFGEESGEMND"
     gene            1777..2181
                     /locus_tag="VMZ53_08250"
     CDS             1777..2181
                     /locus_tag="VMZ53_08250"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HUS28485.1"
                     /translation="MTDSVFVRGLEFEGNHGYTAAERRGTRRFRVNLTLELPLAAAAQ
                     SDKIVDTVDYWKVSEVVVALGTKSTFKLLEALAGAIGAKIQELYPQAAVAIELEKLAP
                     PCPGVPAACGVKLVLPARQAEPGSLHSGKAVS"
     gene            2178..2597
                     /locus_tag="VMZ53_08255"
     CDS             2178..2597
                     /locus_tag="VMZ53_08255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HUS28486.1"
                     /translation="MSFSYSLPVRFADVDHAGIVYYPRFFHYFHVAFEELWRARIGPK
                     AYSDIIDNQRIGFPAVRAECDFKAPLKFGDTAEIEVSIPRLGAKSITFRYRIFRSGDR
                     ALCAEGHVITAVVDLAKFVAVAIPEHVSDMLKDLVES"
     gene            2679..3407
                     /locus_tag="VMZ53_08260"
     CDS             2679..3407
                     /locus_tag="VMZ53_08260"
                     /inference="COORDINATES: protein motif:HMM:NF016286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HUS28487.1"
                     /translation="MVTALIEHRVSTSLVDVERALARWRSGAHSALAAHGAVLRHAAR
                     CERTVERVTAAAGDRPEGILRDAVDAGLMSADEFVLLVGKQPSEVESIDALRDEEDAS
                     APDKRRTLETLLARGPVLVHVDARRAEVQVPARFRADPSLVLRFGYTLSPAIADLEVD
                     DNGIGGTLTFGGQPFHCVLPWTAVYAAMVEGEQRGTVWPEDVPEDVLTGGGETPAAIP
                     APVPAAEEKSAPRPKRASHLKLVE"
     gene            3409..4089
                     /locus_tag="VMZ53_08265"
     CDS             3409..4089
                     /locus_tag="VMZ53_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28488.1"
                     /translation="MRALVALVLVACGSHASHSTLKGSSEPARAAPTTPAPAISFIDD
                     RFEVRGLPAIARDRSIVVVPVIEGDGGRGYPNLALEVRLNNDKPIETLPVMISNDYEA
                     LVANGSAMPELVRRIEKTNARLREIHGKDDLVEMKRSDNLDGRVSYANGTLTLVLDAG
                     RSLASEHPDWKAPQLLRRTNAPPCENPEYLKAVYFAEGINAIVVEIAFEGTDTCWEPG
                     NQLHVVTW"
     gene            4288..6012
                     /locus_tag="VMZ53_08270"
     CDS             4288..6012
                     /locus_tag="VMZ53_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28489.1"
                     /translation="MKRFAWLISLAACGGHAPAPVAPGAAPVQPPRLLPTIAEQTFYG
                     SKASCAQGPFELEIPVGAAKYGEVIELSLLAPRRIALHATITAGDTELATIDDTFDAS
                     GRVGGNADNARCVADARERLAAERARRAGTQGPATTPGSRGSVTTPSPGAPAASPPPP
                     ASVAAELEVVATPTEVVTPVISVRIPPGATTVRIKWWSIQPNDLEGVRFGFSHVVWKP
                     DVGEAAYEVWLAEQETKRIEEQKRLAAEAEMRARATAEIEFKEKRAEDERRREAQLHA
                     KVTQQVVVKQHVETEEQRRVRIEAELRIKVELETRRKRQEADMRAREEAEEQRRLKIE
                     AELRVSEEKRRHAEEAAAEKRRLKIEAELRAAEERKQFCASHPADRSCWGPGGLDMKL
                     QLDARVEQRTAYCSQHHEDARCWTADERRTIAAADEARLTAALAPPSKPTGPPPSPLE
                     ETIPPKLSVNAEWRPGYWHWLDNTWVWLAGMWRVPEQDIVAEQTTTAPIAPPPLQVEA
                     PPAAPVQAAVWVNGFWQWDGAKWLWVAGSWQLRPSATVSWRAATWKQRGTVHVLVPGA
                     WIRRGGSR"
     gene            6009..6722
                     /locus_tag="VMZ53_08275"
     CDS             6009..6722
                     /locus_tag="VMZ53_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28490.1"
                     /translation="MKSLALCLALALASTGCFSTWVGMRVAGHPGTADESVREERVPQ
                     PGLREHLAVSLVGGRFKCTSSQQATDIVYHSGYRYGSRWKRSAAIMFVAEAALATAFY
                     FSNPDDPSKRASYLLAAGFFAADAVGTGVIAFIPRKEIYREETIAVSTPVRDACPEGL
                     SVQIASETYPIDAAGAVGELGETALAEWQQAANGELLVDFEGRTSPLRSDGLVQAAIF
                     DVPAGTLAGVALSTAALSQ"
     gene            6780..8027
                     /locus_tag="VMZ53_08280"
     CDS             6780..8027
                     /locus_tag="VMZ53_08280"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS28491.1"
                     /translation="MVRLARALRHRNYRLFIIGQGTSQIGTWLSKFATSWMAYQLTGS
                     PFMLGLVTFFNNAPTPLIAPFAGVFVDRRNRHKVVILTQILALLQSAALAAFAFTGTM
                     TVWHLIFLGAAQGVINAFDMPARQSFLREMIDDPADLPNAIAINSSMVNGARLVGPAI
                     AAVLVGLVGEAWCFTIDAISYVAVIGSLLVMRIKPRPLAVRTGQVFADIVDGVRYATS
                     VPLVKSVLLMLAVSSVLAGAYSTLLPVVATTTLHGGPYTLGILMSSAGLGALCGALYL
                     ASRSTVLGLGRVIGRTTLGMGIGLVALELATRTWIAAPILFAIGACLMIQMAATNTIV
                     QTITPPDKLGRVMSLYLVAFTGGTPLGSIVEGSLASHIGPLHTFMLAGVGCIAAGLWF
                     RRELPHLRAQTRPLYVQLGILES"
     gene            complement(8028..9302)
                     /locus_tag="VMZ53_08285"
     CDS             complement(8028..9302)
                     /locus_tag="VMZ53_08285"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HUS28492.1"
                     /translation="MARTIDVHIADAPRAVALLRHEERGARESASFEYVAEWLADRER
                     FAIDPTLPLVRGPQFHKRSKEGSLFHNALADTEPDGWARRVILRDHQKRRQAGETVPE
                     RLNELDFLLAVDDATRVGALRFRDERNKFCRAPDGRRRTTPPLVELSALLTSSKAVEL
                     NRETAADLAYLRGRGTSLGGQRPKCSVIDDHGNLSIGKFPSVSDERAVTKGEVLALTL
                     ARKAGIRAAHAELVDADGSPVALIRRFDRSGVKRTLYVSAATLTGVDPHEGSDHSYTE
                     IADAIRVHGDSVQADLEELWRRLAFTILITNVDDHLMNHGFLYATRGRWRLSPAFDVN
                     PFPERARELKLWISEEAGPAATVDGLMSVAPYFKISQQTAKTILRQVEAAVARWRAEG
                     KRLGMSAPELEQFEPAFEHSERAAARKVAATR"
     gene            9338..9532
                     /locus_tag="VMZ53_08290"
     CDS             9338..9532
                     /locus_tag="VMZ53_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28493.1"
                     /translation="MSFVEHHSGFVELGIDDVTEGRSKAENEQRHRIDAERYTRVTFL
                     DAHVGAIADSAHAFAQHRDG"
     gene            complement(9804..10184)
                     /locus_tag="VMZ53_08295"
     CDS             complement(9804..10184)
                     /locus_tag="VMZ53_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HUS28494.1"
                     /translation="MPKITFKNPQGKLSTGPLTVECKRGLSILDAAEECGARVGHACG
                     GNLACSTCHVYVEEGIDSLPEIADKENDIMDKAFDVRPESRLGCQAKLGDEDVVVEIS
                     EESLRAWLDENPEERKKLAAAKAG"
     gene            complement(10184..12232)
                     /gene="hscA"
                     /locus_tag="VMZ53_08300"
     CDS             complement(10184..12232)
                     /gene="hscA"
                     /locus_tag="VMZ53_08300"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HUS28495.1"
                     /translation="MLLQISEPGESTIKEACKPRVVGIDLGTTNSLVAHVADASPEVL
                     GPLSPTGGVDGIVPSVVYYSSNGSSNGANTNHGVLVGREAQAKAIEAPRQTLASVKRL
                     IGRGLKDIGAMRALIPYELTGDDRAVKIKLADREVSPVEVSAEILRALKDRAEQALGG
                     PIEGAVITVPAYFDDAQRQATRDAGRLAGLEVMRLMAEPTAAAVAYGLDKGTKGTYAV
                     FDLGGGTFDISILKLVEGVFEVKATGGDSALGGDDLDRAIAQELFAADLASADLSRIG
                     SAVARGRQLKEQLTDADAVPVRAADATNAGGRSSDATSAGGLSSDATSAGGGAADATF
                     TRADLKRVAQPLLDKCAKACRRVLKDAELSKEELDGVILVGGSTRSPVVRDFVRELFG
                     KEPLCDLDPDQVVALGAAVQADVLAGGEQKVVLLDVVPLSLGLEMMGGVVEKLIHRNT
                     TIPCGATETFTTYADNQTGFDVHVVQGERETVDACRSLARFTLRGIPPMIAGMARLEV
                     TFLVDADGLLKVMAKELTTGQEASIEVKPSYGLTDEEVERMLIESFEYAEDDLAKRNL
                     LIERVEAERILAATKQAFATDTLLLTDDVRAAGEKAMTELQTAIAGDEHLKIRHAIEA
                     LDIATKPFAQARMNRAIEAGFLGKTLEEAEALVPEGRADVHRQHSGDAHAGHDHKESR
                     "
     gene            complement(12263..12793)
                     /gene="hscB"
                     /locus_tag="VMZ53_08305"
     CDS             complement(12263..12793)
                     /gene="hscB"
                     /locus_tag="VMZ53_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HUS28496.1"
                     /translation="MERASNSDAFGLLGLPAQFDLDPQVIERAFHDKSKELHADKFAT
                     APAAERVAALSRSRAINDAYKTLKSPVARAEYLLARAGVTIGDNERLDPAFLMEILEL
                     REELAEARVAKQTSLIEKLCGEMKTRRKASLEKLPALFATNDLHAIKEQLMLLRYVDR
                     YLEECDSALDPDAEEA"
     gene            complement(12771..13175)
                     /locus_tag="VMZ53_08310"
     CDS             complement(12771..13175)
                     /locus_tag="VMZ53_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HUS28497.1"
                     /translation="MSSSPATDTKTPPSPAAAAAVASKKEIIVTENAAQEIARQREKR
                     GTPNARIRVGVRGGGCTGFTYVFEWADSVKPTDKEFKAEGHDIGIVVDPKSLVYLGGM
                     TLDFVRGMMGHGFKFNNPNAKGSCGCGESVQF"
     gene            complement(13181..13564)
                     /gene="iscU"
                     /locus_tag="VMZ53_08315"
     CDS             complement(13181..13564)
                     /gene="iscU"
                     /locus_tag="VMZ53_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HUS28498.1"
                     /translation="MAYSDKVLDHYENPRNVGTFAKDEGEDVGTGLVGAPACGDVMRL
                     QIKVKDGRIEDAKFKTFGCGSAIASSSLATEMIKGMTLDDAAKITNTQIVEELNLPPV
                     KIHCSVLAEDAIKAAIEDYKKKHSR"
     gene            complement(13580..14809)
                     /locus_tag="VMZ53_08320"
     CDS             complement(13580..14809)
                     /locus_tag="VMZ53_08320"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF010611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HUS28499.1"
                     /translation="MSVKTPIFMDNHSTTRVDPRVLETMIPYFDVHFGNAASRNHAFG
                     WTAEAAVEHARTQVAALIGGDGKEIVWTSGATESNNLAIKGAASFYKKQGNHIITVET
                     EHKSVIDTCKRLQREGCEVTYLTPNKDGTVTPEMVTAAMTDKTVVVSIMLANNEIGVV
                     QDVNAIGDAIKKKNKKTLFHIDAVQGAGKIPFDVEAAKADLVALSAHKMYGPKGVGAL
                     WVRRKPRVRIDPIIDGGGHERGMRSGTLAVPLLVAFGKAAELAKDEMPAEAIRLRALR
                     DRLYRGISSKLPETYVNGTMDEGKRLPGNLNISFAFVEGESLLMALKDVAVSSGSACT
                     SASLEPSYVLRALGTGDELAHSSIRFGLGRFNTEEEVDYVIDLVVKSVERLRALSPLW
                     EMHQEGIDINSIQWTPH"
     gene            complement(14810..15373)
                     /locus_tag="VMZ53_08325"
     CDS             complement(14810..15373)
                     /locus_tag="VMZ53_08325"
                     /inference="COORDINATES: protein motif:HMM:NF020839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUS28500.1"
                     /translation="MTDGTNPKLMRVGELAKAVGKTVRAMHLYEELGLLEPRARSEGG
                     FRLYGPEAVDRIHWIVKLQAIGFTLAEIQGFVRDFQSAGSAPEASARVRALFLEKQQN
                     IREQITQLQVIENDLSEALAYLDSCQTCSTDFAPTECGTCDHHGHHKGEAPPLFAGLS
                     PKRPQDGDKGEKAFDVPVTVLYREGNN"
     gene            complement(15533..16297)
                     /locus_tag="VMZ53_08330"
     CDS             complement(15533..16297)
                     /locus_tag="VMZ53_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28501.1"
                     /translation="MRALAVLIVVAGCAKTASELSPFPCADDLTCPAGLGCVAGQCQT
                     AVLDAICTAGSDPTDCSVAAAGAVCSKQSTVLDIGACEVPCGAGCAAGRACSFSDNHG
                     FCLVDCNGSSTVCPPNTICLPRDDGHKVCTPPSIGCRTVSNARRCTSRFCESRSTEVT
                     CPDGVATCPEGATCSSNSRSCTCDDNRQPIACDNDQPCNSACGGNRHWWCAPQYDASC
                     FGDLTDFQLTCECWAGSVVVDCDRQGVTCDELCRDL"
     gene            complement(16294..>17250)
                     /locus_tag="VMZ53_08335"
     CDS             complement(16294..>17250)
                     /locus_tag="VMZ53_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28502.1"
                     /translation="RPRYPATSMRWMVVVLLCVNVAYAQPAPDAKAVVTPAQKREAAA
                     HRAKAKAFVAKKQRDKAIVELEASVAILPTDDALYELATLYDQIPDEDKAFSAYQRIA
                     GGNHLSDASARLKAIIEARTQRAADAAAKAEAEAKAKAEAEAKARTEAEARARRTAEE
                     SRQRAEREDRDRRDAEANAREAALRKSIESRHDALVLGAKRAEWDVDREKRRERRARG
                     KRYLRIGVACGAVAGVAAGIAALQQSRVDNGGFATASDISFAISSARIATYAAWAFGV
                     PAAVGIGVGLPLLVLGRDRGEMRASVVANDGMQGIALSGEWR"
BASE COUNT         2873 a         5740 c         5521 g         3116 t
ORIGIN      
        1 gttgtgctcc gacgcgccga cccaatgcgc ttcgagtcct tcgacagaca cgcccgcttg
       61 ctcgaggccc gcgcgcttcg ccggattgtt cgtcatgagg cggacgctgg tcacgccgag
      121 gtcgcgcagg atgccaaccg cgatctcgta gcttcgatca tcagcgccga aaccgagcgc
      181 gaggttcgct tcgaccgtgt ccttgccttc gtcctgcagc tggtacgcgc ggaccttgtt
      241 gccaaggccg atgccgcggc cttcttgcac gagatagacg accacgccgc ggccttcgag
      301 cgcgatgcga gcgagtgcgg cgtcgagttg cgcgcggcaa tcgcagcgca ggctgccgag
      361 cacttcgctc gtgaagcacg acgagtgaac gcgacagagc acgcccggtc cggcgacctc
      421 gcccatcacc attgcgacgt gctcttcatc ggcaagtccg accgctgtct ggccgagtcc
      481 gcccggccta ccagcccgat acacgaccac ccgaaattcg ccgtgtggcg tcggaagtcg
      541 agcgtcggaa tacctcgtaa gcattggtgg ggcgtgtatg gctgggtccc gtcgggttgt
      601 caatcagccc tgtggcgcgc gtgctaaggt tggccggtca tggctgttcg tcccaccgtc
      661 atctggcccg atccgaggct gcgcgaaccg accatcgcca tctcgacgat cgacgagtcg
      721 gtccgcgagc tttaccgaga tctgtgcgac tcgatgtacg cggagaatgg gctcggcatg
      781 gcggcgcccc agatcggcga caaccgcaag atgttcatcg tcgagccgaa gctcgccggc
      841 atggcggaga cggaccagcc ggtctgcctg atgaacgccg aagtgatcga gacgtcggaa
      901 gagacgcaag acagcgaaga aggctgcctc tcgttccccg acatctacgt gaaggtgaag
      961 cgtccgatgc gctgcaaggt ccgttacctg ggcctcgatg gacagctgca cgagatcgat
     1021 ggcgaagcgc tgatggcgcg ttgcctgctg cacgagaacg atcacctcac gggaaagctg
     1081 ctcgtcgatt tcgtcggccc gctcaagcgg cagatgatca agaagaagct gcagcgcgtg
     1141 aaagacgccg aagccgacga agcggccggc tagctaatcg ttcatctcgc cgctctcttc
     1201 accaaacggc gtcgggctcc agatcacgcg ctgcacgatc tcaccgtcga gcgcgcgcag
     1261 tgcgctgcga tcgctacctg tgaggtcctc gcagtcgcgc ttgaagttca cggcgatgtg
     1321 ctcgccgctc gcgtagcacg tcgggttcgg gccccagcat tcgcagttgg tcaggtgcgc
     1381 tgaatcgccg aacgcgcggc cgacgcgcca cgggtgtccg ctgaccacga cgcgcgagct
     1441 cgtcgcatgc acgttgaaga cgtagccgct ctgatcgtcg ggcacgacat acgcgagctt
     1501 ctcgccgtcg cgatagatgt cgtactcgag cgaggtgtcg ttgagcgggc gaacctcgta
     1561 gccgatcaac agcgcgcgca ggttcgtcag cgtgaccttc gtcgtgttgt cgatcggacc
     1621 gacgcccttc ggcgtcatcg tcagcgcggc cggcgccgca tccggtgtct tgttcgcgag
     1681 cggcgtggcg cccggcgtcg accgataaca accggcgagc aagatcagcc atgcgacgcg
     1741 catgcgcgca ttgtagctgc ggtataggtt gccgcgatga ccgacagcgt cttcgtgcga
     1801 ggcctcgagt tcgagggcaa tcacggctac accgcggccg aacgccgcgg cacgcgccgg
     1861 tttcgcgtga acctgacgct cgagcttcca ctcgccgcgg ccgcgcagtc cgacaagatc
     1921 gtcgataccg tcgactactg gaaggtgagc gaggtcgtcg tcgcgctcgg cacgaagtcg
     1981 acgttcaagc tgctcgaagc gctcgccggc gcgattggcg caaagattca agagctgtat
     2041 ccgcaggcag cggtcgcgat cgagctcgag aagctcgccc cgccctgccc cggcgtgccc
     2101 gcggcgtgcg gcgtcaagct ggtcctcccc gctcgccagg cagagcccgg ctcgctccat
     2161 tctgggaaag cggtgtcatg agcttttcgt actcgctgcc ggtgcggttc gcggacgtcg
     2221 atcacgcggg catcgtctac tacccgcggt tctttcatta ctttcacgtc gcgttcgaag
     2281 agctgtggcg cgcgcgcatc gggcccaagg cgtactcgga catcatcgat aaccagcgca
     2341 tcggctttcc cgccgtccgc gcggagtgcg acttcaaagc tccgctcaag ttcggcgaca
     2401 ccgctgagat cgaagtgtcg atccctcggt tgggcgcgaa gtcgatcaca tttcggtaca
     2461 gaatttttag gtcaggtgat cgcgcgctgt gcgcggaagg ccatgtgatc accgcggtcg
     2521 tggacctcgc taaattcgtt gcggtggcca ttccggagca cgtctcggac atgttgaaag
     2581 acctggtcga atcctgagtt gacgcccgct ccccagggag tgtaagcccc ctgaagatga
     2641 tcgagcggga ccccaagact ttggcaaggc tgcaggcggt ggtgacggct ctgatcgagc
     2701 atcgcgtgtc gacgtcgctc gtcgatgtcg agcgtgcgct ggcccgctgg cgctccggtg
     2761 cgcactcggc cctcgccgcc cacggtgcgg tccttcgcca tgcggctcgc tgcgagcgca
     2821 cggtcgagcg tgtgacggcc gccgccggcg atcgtcccga aggcatcctg cgcgacgccg
     2881 tcgatgcagg cctgatgtcg gccgatgaat tcgtgctgct cgtcggcaag cagccgagcg
     2941 aggtcgagtc gatcgacgcc ctccgcgatg aagaagatgc cagcgcgccg gacaagcgcc
     3001 gcacgctcga gacgctgctc gcccgcggtc ccgtgctcgt tcacgtcgat gcgcgccgcg
     3061 ccgaggtgca agttccagct cggttccgcg ccgacccatc gctcgtgctt cgcttcggct
     3121 acacgctgtc ccccgcgatc gccgacctcg aagtcgacga caacgggatc ggcggcacgc
     3181 tgacgttcgg cggccaaccg ttccactgcg tgttgccgtg gaccgcggtc tacgccgcga
     3241 tggtcgaagg cgagcaacgc ggcacggtgt ggccggagga tgtgcccgaa gacgtgctca
     3301 ccggcggtgg cgagacgccc gccgcgattc ccgcgcctgt gcccgcagcc gaagagaagt
     3361 cggccccgcg tccgaagcgc gcgagccacc tgaagctcgt cgagtaacgt gcgcgcgctc
     3421 gtcgcgcttg tgctcgtcgc ttgcggatcg cacgcgtcgc actcgacgct gaagggcagc
     3481 tccgagccgg cgcgcgccgc accgacgacg cccgcgccag cgatctcgtt catcgatgat
     3541 cgcttcgagg tccgtggcct tcccgcgatc gcacgcgacc gttcgatcgt cgtcgtgccg
     3601 gtgatcgagg gggacggtgg ccgcggttat ccgaacctcg cgctcgaagt ccggttgaac
     3661 aacgacaagc cgatcgagac cttgcccgtg atgatctcga acgactacga agcgctcgtc
     3721 gcaaatggct ccgcgatgcc cgagctcgta cgccgcatcg agaagacgaa cgcgcggctg
     3781 cgcgagatcc atggaaagga cgacctcgtc gagatgaagc gcagcgataa cctagacggc
     3841 agggtttcat acgcaaacgg cacgttgacg ctcgtgctcg atgcaggccg ctcgctcgcg
     3901 tcggagcatc ccgactggaa agcgccgcag ctactgcgcc ggaccaacgc tccaccgtgc
     3961 gagaaccccg agtacttgaa ggcggtctac ttcgcagaag ggatcaacgc gatcgtcgtc
     4021 gagatcgcat tcgagggtac cgacacgtgt tgggagccag gcaaccagct gcacgtcgtc
     4081 acgtggtgat ggcgcgttcg accccggccc tggcgcgata agaacgttcg acgcacgagg
     4141 cggcgcgcgc gtaacctttc tttttcgacg gaaaccgaag gcaattcgcg caacacctcg
     4201 gcgtgaacgc tgagacccga cgttcacgtt cgtcgacgaa atcccgcgca gatccgcgtg
     4261 gcacgtcgcg tgctgagacg gtctcgcatg aagcggttcg cttggcttat ttcactcgcg
     4321 gcgtgcggag gccacgcgcc cgcgccggtc gcgcccggag ctgcgccggt tcaaccgccg
     4381 cgcctcctgc cgacgatcgc cgaacagacg ttctacggca gcaaggcgag ctgcgcgcag
     4441 ggcccattcg agctcgagat tccggtcggc gcggcgaagt acggcgaggt catcgagctc
     4501 tcgctgctcg cacctcggcg catcgcgctt cacgcaacga tcaccgcggg cgacaccgag
     4561 ctcgcgacca tcgatgacac gtttgacgcc agcgggcgcg tcggaggcaa cgcggacaac
     4621 gcgcgttgtg tcgccgacgc tcgcgaacgt ctcgccgccg aacgcgcgag acgcgcaggc
     4681 acccaagggc cggcgacaac gccaggctct cgcggatcgg tcaccacgcc gagcccgggt
     4741 gcgcccgctg caagcccgcc acctccagcg tcggttgccg ccgagctgga ggtggtggcg
     4801 acgccgacgg aggtcgtcac gccggtcatc tccgtccgca tcccgccggg cgcaaccacc
     4861 gtgcggatca agtggtggag catccagccc aacgatctag agggtgtgcg ctttggcttc
     4921 tcgcacgttg tctggaaacc ggacgtcgga gaagcggcgt acgaagtgtg gctggccgag
     4981 caggagacga agcgcatcga ggagcagaag cggctcgccg ccgaagccga gatgcgtgca
     5041 cgcgctaccg cggagatcga attcaaggag aagcgcgcgg aagacgagcg ccgtcgcgag
     5101 gcacagctcc atgcaaaggt gacccaacag gtcgtcgtga agcagcacgt cgagaccgag
     5161 gagcagcgcc gggtgcgtat cgaggccgag ctccgcatca aggtcgagct cgagacgcgt
     5221 cgcaagcggc aggaagccga tatgcgcgca cgcgaggagg ccgaagagca gcgccgtctg
     5281 aagatcgagg ccgagctccg cgtgtccgag gagaaacgcc ggcacgccga agaagccgcg
     5341 gccgagaagc gccgtctgaa gatcgaagcg gagctgcgcg cggccgaaga gcgcaagcag
     5401 ttctgcgcga gtcatccggc cgaccgcagc tgctggggcc ctggcggtct cgacatgaag
     5461 ctccagctcg acgcgcgtgt agagcagcgc accgcgtact gctcgcagca tcacgaagac
     5521 gcgcggtgct ggaccgccga cgagcgccgc acgatcgcgg ccgccgacga ggcgcggttg
     5581 acagcggcac tcgcgccgcc gagcaagccc actggtccgc cgccctcgcc gctcgaggag
     5641 accatcccgc ccaagctcag cgtgaatgcg gagtggcgcc ccggttactg gcattggctc
     5701 gacaacacct gggtgtggct cgccggcatg tggcgcgttc ccgagcaaga catcgtcgcg
     5761 gagcagacga cgaccgcgcc gatcgcaccg ccgccgctcc aggtcgaggc accgcctgcg
     5821 gcgcccgtgc aagccgcggt gtgggtcaac ggcttctggc aatgggatgg tgcgaagtgg
     5881 ctgtgggtcg caggctcgtg gcagctgcgt ccgtcggcga cggtgagctg gcgcgcagcg
     5941 acgtggaagc aacgcggcac cgtgcacgtg ctcgttcccg gcgcatggat ccgaagagga
     6001 ggttcgcgat gaagtcgctc gctttatgtc tcgcgctcgc cctcgcatcg acgggctgtt
     6061 tctcgacgtg ggtcggcatg cgcgtggccg gacatcccgg caccgcggac gagagcgtgc
     6121 gcgaagagcg cgtgccgcag cctgggttgc gcgagcacct cgcggtgtcg ctcgtcggcg
     6181 gtcgcttcaa gtgcacgtcg agccagcagg cgacggacat cgtctatcac tcgggctatc
     6241 gctacggctc gcggtggaag cgaagcgccg cgatcatgtt cgtcgccgaa gccgcgctcg
     6301 cgacagcctt ctacttcagt aaccccgacg atccgagcaa gcgcgcgagc tatctcctcg
     6361 ctgcaggctt tttcgccgcg gatgcggtcg gcacgggcgt gatcgccttt atcccgcgca
     6421 aggagatcta tcgcgaggag acgatcgcgg tgtccacgcc ggtgcgcgac gcctgtcccg
     6481 agggcctctc cgtgcagatc gcgagcgaga cctacccgat cgatgcagcc ggcgcggtcg
     6541 gcgagctcgg cgaaacggcc ctcgcggaat ggcagcaagc cgccaacggt gaattgctcg
     6601 tcgatttcga aggcagaacg tcgccgctgc gctcggacgg gctggtccag gcggcgatct
     6661 tcgacgtgcc ggcgggcact cttgcgggcg tcgcgctgtc cacggccgcg ctttcgcaat
     6721 agctcgcacc tgacgaggcg ttacgagcat cgaagacgcg taggagctac cctcgtttta
     6781 tggtgcggct cgcacgcgcc ctcaggcatc gcaattaccg gctgttcatc atcggccagg
     6841 gcacgtcgca aatcggcacg tggctctcga agttcgcgac gagctggatg gcatatcagc
     6901 tgaccggctc gccgttcatg ctcggtctcg tcacgttctt caataacgcg ccgactccgc
     6961 tcatcgcgcc gttcgctggc gtgttcgtcg atcggcggaa ccgccacaag gtcgtcatcc
     7021 tcacgcagat cctcgcgctg cttcaatcgg cagcgctcgc cgcgttcgcg ttcaccggaa
     7081 cgatgacggt ctggcacctg atcttcctcg gtgccgcgca aggcgtgatc aacgcgttcg
     7141 acatgccagc gcggcagagc ttcttgcgcg agatgatcga cgacccagcg gatctgccga
     7201 acgcgatcgc gatcaactcg tcgatggtca acggcgcgcg cctcgtcggt ccggcgatcg
     7261 ccgccgtgct cgtcggcctc gtcggcgaag cgtggtgctt cacgatcgat gcgatcagtt
     7321 acgtcgccgt gatcggttcc ttgctcgtca tgcggatcaa gccgcggccg ctcgcggtcc
     7381 gcacgggcca agtcttcgca gacatcgtcg acggcgtgcg ctacgcaacg tcggtccctc
     7441 tcgtcaaaag cgtgcttctc atgctcgccg tctcgagcgt cctcgccggc gcgtactcga
     7501 cgctgctgcc cgtcgtcgca accaccacgc tgcacggcgg tccgtacacg ctcggcatct
     7561 tgatgtcgtc ggcgggcctc ggcgcgctgt gcggcgccct ctacctcgca agccgctcga
     7621 cggtgctcgg cctcggccgc gtcatcggcc gcacgacgct cggcatgggc atcggcctcg
     7681 tcgcgctcga gctcgccacg cgcacgtgga tcgccgcgcc catcctgttc gcgatcggcg
     7741 cgtgcctgat gatccagatg gcagcgacga acacgatcgt tcaaaccatc acgccacccg
     7801 acaagctcgg ccgcgtcatg agcctctacc tcgtcgcgtt cacgggcggc acgccgctcg
     7861 gctccatcgt cgagggctcc ctcgcgagcc acatcggccc gctgcacacg ttcatgctcg
     7921 ccggtgtggg ctgcatcgcc gcgggcctgt ggttccggcg cgagctgccg cacctgcgcg
     7981 cccaaacacg cccgctctac gtgcagctcg gcatcctcga gagctgatca acgcgtggcg
     8041 gccaccttcc gtgccgcggc tcgctcggaa tgctcgaacg ccggctcgaa ctgctcgagc
     8101 tctggcgcgg acatcccgag cctttttcct tccgcccgcc atcgcgcaac tgcagcttca
     8161 acctggcgaa ggattgtctt ggctgtctgc tgagaaatct tgaaataggg agcgacggac
     8221 atcaggccgt ccacggttgc ggccgggccc gcctcctccg agatccagag ctttagctcg
     8281 cgcgcacgct ccgggaaggg gttgacatcg aacgcaggag ataaccgcca tctgcctctc
     8341 gtcgcgtaca agaagccgtg attcatgagg tgatcatcga cgttggtaat gaggatcgtg
     8401 aatgccaagc ggcgccacag ctcctcgaga tccgcttgca cggaatcacc atggacgcgg
     8461 atcgcatctg caatctccgt gtacgagtga tcgctccctt cgtggggatc cacacccgtc
     8521 agcgtcgccg ctgatacgta gagcgttctc ttcacgccgg agcgatcgaa gcgacgaatg
     8581 agcgcgacag gactcccgtc agcgtcgacg agctcggcgt gcgcggcacg aatgcctgcc
     8641 ttccgtgcca acgtcaacgc gagcacctcg ccctttgtga ccgcccgctc gtcactgacg
     8701 ctggggaact ttccaatcga aagatttccg tggtcgtcga taacgctgca cttcggccgc
     8761 tgcccgccga gagaggtccc gcgaccacgt agatacgcaa ggtctgcggc cgtctctcgg
     8821 ttcagctcta ctgcttttga tgatgtaagc agtgcgctca gctcgaccag tggcggtgtt
     8881 gtgcgacgcc gcccatccgg cgcgcgacag aacttgtttc gctcgtcgcg aaatcgaagc
     8941 gcgccgacac gcgtcgcgtc atcgactgcg agtaggaaat cgagctcgtt aagccgctcc
     9001 ggcacggtct cgcccgcttg gcgacgcttc tgatgatctc gaagaatcac gcgccgagcc
     9061 cagccgtccg gctcggtgtc cgctagcgcg ttgtgaaaca gcgagccttc cttgctacgt
     9121 ttgtgaaact gaggaccacg aacgagtggc agtgtcgggt cgatcgcgaa ccgttcacgg
     9181 tccgcgagcc attcggcaac gtactcgaac gaggcacttt cacgcgcgcc tcgttcttca
     9241 tggcgcagca atgcgaccgc tcgtggcgca tcggcgatat ggacgtcgat cgttcgtgcc
     9301 atcacccact cacgcgtgaa cgaatcctct tggggaggtg agcttcgtcg agcaccattc
     9361 cggtttcgtc gagcttggga tcgacgatgt caccgaaggc cgatccaaag ccgagaacga
     9421 acagcgccat cgcatagatg ccgagcgcta caccagggtc acctttctcg acgcgcacgt
     9481 aggtgcgatt gctgattccg cccatgcgtt cgcacagcat cgcgacggtt agacgtcttc
     9541 tcagacgcgc ggtccgaatg tcggtaccga gcttggccaa agaccgccga acggccgacg
     9601 ggaggactgc agcaacgctg gaccgcataa cgtttactat aatacacgct atgaggggta
     9661 ccgtgtacca aagtaaacgt gtggcgggcg tacgtcacga cataacgttt acccgacagc
     9721 atgttatggc tgattacgtg tcatatagta cacgttagcg gcggaatgct gcattgaagg
     9781 gcacttttgc cccaggcgcg tgtctagccg gccttcgcag ccgcgagctt cttgcgctct
     9841 tcaggatttt cgtcgagcca cgcgcgcagc gactcttcgc tgatctcgac gacgacatcc
     9901 tcgtcgccga gcttcgcctg gcagccgagg cgcgactcgg gtcgcacgtc gaacgccttg
     9961 tccatgatgt cgttctcttt gtcggcgatc tcgggcagcg agtcgatgcc ctcctcgacg
    10021 tagacgtgac acgtcgagca cgcgaggttg ccaccacacg cgtgaccgac acgcgcgccg
    10081 cactcttccg ccgcatcgag gatcgacagg ccacgcttgc actcgacggt gagcggcccc
    10141 gtcgacagct tcccctgcgg gttcttgaac gtgatcttcg gcatcagcgc gattccttgt
    10201 ggtcgtggcc cgcgtgcgcg tcgcccgagt gttggcgatg gacgtcggcg cggccttctg
    10261 ggacgagtgc ctcggcttcc tcgagcgtct tgccgagaaa gcccgcctcg atcgcgcgat
    10321 tcatgcgcgc ctgcgcgaac ggcttcgtcg cgatatcgag cgcctcgatc gcgtggcgaa
    10381 tcttcagatg ctcgtcgccc gcaatcgcgg tctgcagctc cgtcatggcc ttctcgcccg
    10441 ccgcgcgaac gtcgtcggtc agcagcaaag tgtccgtcgc aaatgcctgc ttcgtcgcgg
    10501 cgaggatgcg ctcggcttcg acgcgctcta tcagcagatt gcgcttcgcg aggtcgtcct
    10561 cggcgtactc gaagctctcg atcaacatcc gctcgacttc ctcgtcggtg agcccgtagc
    10621 tcggcttcac ctcgatcgag gcctcttgcc ccgtcgtcag ctccttcgcc atgaccttca
    10681 gcaagccatc ggcgtcgacg aggaacgtca cctcgagccg cgccatgccc gcgatcatcg
    10741 gtgggatgcc gcgcagtgtg aaccgcgcga gcgagcggca cgcgtctacg gtctcgcgct
    10801 cgccctgcac gacgtgcacg tcgaagccgg tctggttgtc ggcgtacgtc gtgaacgtct
    10861 cggtcgcgcc gcacggaatc gtcgtgttgc gatggatgag cttctcgacg acgccgccca
    10921 tcatctcgag gcccagtgac agcggaacaa catcgaggag gacgaccttc tgctcgccgc
    10981 ccgccagcac atcggcctgc accgccgcac cgagcgcgac gacctgatct ggatcgagat
    11041 cacaaagcgg ttccttgccg aatagctcgc gcacgaagtc gcgcacgacc ggcgatcgcg
    11101 tcgatccccc gacgaggatc acgccgtcga gctcctcttt cgacagctcg gcatctttga
    11161 gcacgcgcct gcacgccttc gcacacttgt cgagaagcgg ctgcgcaaca cgcttcagat
    11221 cggcacgcgt gaaggtcgcg tccgcggccc cgccacccgc gcttgtcgcg tccgaggaca
    11281 ggccacccgc gctcgtcgcg tccgaggacc ggccacccgc gtttgtcgcg tccgcagcgc
    11341 ggacagggac tgcatcggcg tcggtgagct gctctttcag ttggcgtccc cgcgcaacgg
    11401 cgctgccaat gcggctgaga tcggcgctgg cgaggtcggc ggcgaacagc tcctgcgcga
    11461 tcgcgcggtc gaggtcgtcg ccgccgagcg cggagtcacc gccggtcgcc ttcacctcga
    11521 agacgccttc gacgagcttt aggatcgaga tgtcgaacgt gccgccgccg aggtcgaaga
    11581 ccgcgtaggt gcccttggtg cccttatcga ggccgtaggc gactgcggcg gcggtgggct
    11641 cggccatcag gcgcatcact tcgaggcccg cgagccggcc ggcatcgcgc gtggcttggc
    11701 gctgcgcatc gtcgaagtac gccggcactg tgatcacggc gccttcgatc ggtccgccga
    11761 gcgcttgctc tgcgcgatcc ttgagtgctc tcagaatctc ggccgagacc tcgacgggcg
    11821 agacctcgcg gtcggcgagc ttgattttca ctgcgcggtc gtcgccggtg agctcgtacg
    11881 gaatgagcgc gcgcatcgcg ccgatgtctt tgaggccgcg gccgatcagg cgcttcaccg
    11941 acgcaagcgt ctgcctcggc gcctcgatgg ccttggcctg cgcctcacgc ccgacgagga
    12001 caccgtgatt ggtgttcgcg ccattcgacg agccattcga cgagtagtaa acgaccgacg
    12061 gcacgatgcc atcgacgccg cctgtcggtg acagcgggcc gagcacctcc ggcgaagcgt
    12121 cagcgacgtg tgcgacgagg gagttcgtcg tgccgaggtc gatgccgacg acgcgaggct
    12181 tgcaggcctc tttgatcgtc gattcgccgg gctcggagat ttgcagcaac atgacttagt
    12241 cgttcccggt cgcctgagtg aatcaggctt cctcggcatc ggggtcgagc gcggagtcgc
    12301 actcctcgag atagcgatcg acgtagcgca gcagcatcaa ctgctctttg atcgcgtgga
    12361 ggtcgttcgt cgcgaacagc gcgggcagct tctcgagcga cgctttgcgg cgcgtcttca
    12421 tctcgccgca cagcttctcg atgagcgagg tctgcttcgc gacacgcgct tccgccagct
    12481 cttcgcgcag ctcgaggatc tccatcaaga acgccgggtc gaggcgctcg ttgtcgccaa
    12541 tcgtgacgcc cgcgcgggcg agcagatact cggcgcgagc gactggggac ttcagcgtct
    12601 tgtacgcgtc gttgatcgcg cgcgagcgcg agagcgcagc cacgcgttcg gcagcgggtg
    12661 ccgtcgcgaa cttgtccgcg tgcagctcct tcgatttgtc gtgaaaggcg cgctcgatca
    12721 cctgcggatc gagatcgaac tgcgcgggga ggccgagcag cccgaacgca tcagaattgg
    12781 acgctctctc cacagccaca cgaccccttg gcgttggggt tattgaactt gaagccgtga
    12841 cccatcatgc cgcgcacgaa atcgagggtc atgccgccga ggtacacgag gctcttcggg
    12901 tcgacgacga tcccgatgtc gtggccttcg gccttgaact ctttgtcggt cggcttgacc
    12961 gagtcggccc actcgaacac gtacgtgaag cccgtgcagc cgccgccgcg aacaccgacg
    13021 cgaatgcgcg cattcggcgt gccccgcttc tcgcgctggc gcgcgatctc ctgcgccgcg
    13081 ttttcggtca cgatgatttc cttcttcgac gcaacagctg ctgctgctgc cggcgaaggc
    13141 ggagtcttcg tgtcggttgc tggagaggac gacatggtgt tcaccgcgaa tgcttcttct
    13201 tgtagtcctc gatggccgcc ttgatcgcgt cttcggcgag caccgagcag tggatcttga
    13261 ccggcgggag gttgagctcc tcgacgatct gcgtgttcgt gatcttggcc gcgtcgtcga
    13321 gcgtcatgcc cttgatcatc tcggtcgcga gcgagctcga cgcgatcgcc gagccgcagc
    13381 cgaaggtctt gaacttcgca tcctcgatgc gaccgtcctt gaccttgatc tgaagacgca
    13441 tgacatcgcc gcaggccggc gcgccgacga gacccgttcc gacatcttcg ccttcgtctt
    13501 tcgcgaaggt gccgacgttg cgggggttct cgtagtggtc gaggactttg tctgaatacg
    13561 ccatggctag ttcattccct cagtgtggag tccactggat ggagttgatg tcgatacctt
    13621 cttgatgcat ctcccagagc ggagacagcg cgcgcaggcg ctcgacggac ttcacgacga
    13681 ggtcgatcac gtaatcgact tcctcttcgg tgttgaagcg gccgaggccg aagcgaatcg
    13741 acgagtgcgc gagctcgtca ccggtgccaa gggcgcgcag cacgtagctc ggttcgagcg
    13801 aggccgacgt acacgccgaa ccgctcgaca cggcgacatc cttgagcgcc atcagcagcg
    13861 actcgccctc gacaaacgca aacgagatgt tgaggttgcc cggcagacgc ttgccctcgt
    13921 ccatggttcc gttcacgtac gtctcgggca gcttgctcga gatgccacga tagagccggt
    13981 cacgcagagc gcgcaggcga attgcctcgg cgggcatctc gtccttcgcg agctcggccg
    14041 ccttgccgaa ggcgacgaga agcggaacgg cgagcgtgcc cgagcgcatg ccacgctcat
    14101 gaccaccgcc gtcgatgatc ggatcgatgc gaacgcgcgg cttgcggcgg acccacagcg
    14161 cgccgacacc ctttggtccg tacatcttgt gcgcggagag cgcgaccaga tcagccttcg
    14221 cggcctcgac gtcgaaggga atcttgccgg cgccctgaac agcgtcgata tggaacaagg
    14281 tcttcttgtt ctttttcttg atagcgtcgc caatcgcgtt gacgtcctga acgacaccga
    14341 tctcgttgtt cgcgagcatg atgctcacga cgacagtctt gtcggtcatc gcggcggtca
    14401 ccatctcggg cgtgaccgtg ccgtccttgt tcggcgtcag gtacgtgacc tcgcaaccct
    14461 cgcgctgcag gcgcttgcag gtatcgatca cgctcttgtg ctcggtctcg acggtgatga
    14521 tgtggttgcc ctgcttcttg tagaagctcg ccgcaccctt gatcgcgagg ttgttcgact
    14581 ccgtcgcgcc actcgtccag acgatctctt tgccgtcgcc gccgatgagc gctgcgacct
    14641 gcgtgcgcgc atgttcgacc gccgcttccg ccgtccagcc aaacgcgtgg ttgcggctcg
    14701 cggcgttgcc gaagtgcacg tcgaagtacg gaatcatcgt ctcgagcacg cgcgggtcga
    14761 cacgcgtcgt cgagtggttg tccatgaaga tgggagtctt gaccgacatc tagttattgc
    14821 cctcgcgata gagaaccgtg acgggaacgt cgaatgcctt ctcgcctttg tcgccgtctt
    14881 gcggacgctt cggagagaga ccggcgaaca gcggtggcgc ttcgcccttg tgatgaccgt
    14941 ggtggtcgca cgtaccgcac tccgtcggcg cgaagtccgt ggagcacgtc tgacagctat
    15001 cgagataggc gagcgcctcg ctcagatcgt tctcgatcac ttgcagctgc gtgatctgct
    15061 cgcggatgtt ctgctgcttc tcgagaaaca gcgcgcgcac gcgagcactc gcttcgggtg
    15121 cagagcccgc ggactggaag tcgcgcacga agccctgaat ctcggcgagc gtgaagccga
    15181 tcgcctggag cttgacgatc caatggatgc ggtcgaccgc ctcggggcca tagagacgga
    15241 agccgccctc ggaccgagcg cgcggctcga gtaagccaag ctcctcatag aggtgcatgg
    15301 ctcgaacggt cttgccgacc gccttggcca gctcgcccac tcgcatcagt tttggattgg
    15361 tcccgtcggt catgatcgaa ccctcacgtt aacgtaaggt tctatgtatc cgaagggact
    15421 atggtcgcgt aaaggcaggg tgtcaatggc gaacctcacc tttacgtgag aatgcgatgc
    15481 cgtgcgtcgt cttctgatcc tcgtcgtggt ttcggggtgt tacgcgccga ggtcagaggt
    15541 cgcgacatag ctcgtcgcac gtgacgccct gtcgatcgca atcgacgacg acgctccctg
    15601 cccaacactc gcacgtcagt tggaagtccg tcagatcgcc gaagcaggac gcatcgtatt
    15661 gcggcgcgca ccaccaatgc cgattgccgc cacacgcgct gttgcacggc tgatcgttgt
    15721 cacacgcgat cggctggcga ttgtcgtcgc acgtgcacga ccggctgttc gagctgcacg
    15781 tcgcgccttc cggacacgtc gcaacaccgt cgggacacgt gacctccgtg gagcgcgatt
    15841 cgcaaaagcg gctcgtgcag cgtcgcgcgt tcgagactgt gcggcagccg atcgaaggtg
    15901 gcgtgcagac cttatggccg tcgtcgcgcg gcaagcagat ggtgttcggc gggcacaccg
    15961 tcgagctgcc gttgcagtcg acgagacaga agccgtggtt gtccgagaag ctacacgcgc
    16021 gtcccgccgc acagccggcg ccgcaaggca cctcgcaagc gccgatatcg agcacggtac
    16081 tctgcttgct gcacacggcg cccgctgctg cgaccgagca atccgtcgga tcgctgccgg
    16141 cggtgcagat cgcatcgagg accgcggtct ggcactgccc cgctacgcac ccgagccccg
    16201 ctggacacgt gagatcatct gcgcacggaa acggcgatag ctcgctcgcg gtcttcgcgc
    16261 acccggcgac gacgatcagc accgcgagcg ccctcatcgc cactcgcccg agagcgcgat
    16321 tccctgcatg ccgtcgttcg cgacgacgct cgcgcgcatc tccccgcggt cgcgaccgag
    16381 gacgagcagc ggcagaccga cgccgatgcc gacggcggcg gggacgccga atgcccacgc
    16441 cgcgtacgtc gcgatgcgag cactcgagat cgcaaacgag atgtcgctcg cggtcgcgaa
    16501 cccgccgttg tcgacgcgcg actgctgcag cgccgcgatg ccagctgcga cacccgcaac
    16561 tgcgccacac gcgactccga tccggagata ccgcttgccg cgcgcacgcc gctcgcgacg
    16621 cttctcgcga tcgacgtccc actccgcgcg cttcgcgccg agcacgaggg catcgtgtcg
    16681 cgactcgatc gacttgcgca gcgccgcttc gcgagcattc gcttcggcgt cgcggcggtc
    16741 gcgatcttcg cgttccgcac gttggcggct ctcttccgcg gtacgacgcg cgcgggcttc
    16801 ggcctcggtc ctcgcctttg cctccgcttc cgcctttgct ttcgcttccg cctcggcctt
    16861 cgctgcagca tccgcggcgc gttgcgtacg tgcttcgatg atcgctttca ggcgcgcgct
    16921 ggcgtcagag aggtggttgc ctccggcgat gcgctgatac gcggagaacg ctttgtcttc
    16981 gtcgggaatc tgatcgtaga gagtcgcgag ctcgtagagc gcgtcatcgg tcgggaggat
    17041 cgcgacggag gcttcgagct cgacgatcgc tttgtcacgc tgcttcttcg cgacgaacgc
    17101 cttcgccttc gcacgatgcg ccgcagcttc gcgcttctgc gcgggcgtga cgacggcttt
    17161 cgcgtcgggc gcgggttgcg cgtacgcgac gttcacgcac aacaacacga cgaccatcca
    17221 ccgcatgctc gttgcaggat accgcggtct
//
LOCUS       DATLBZ010000157         4009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9086619, whole genome shotgun sequence.
ACCESSION   DATLBZ010000157 DATLBZ010000000
VERSION     DATLBZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9086619"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VMZ53_08340"
     CDS             complement(<1..920)
                     /locus_tag="VMZ53_08340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUS28503.1"
                     /translation="MIRALTPFHAVDLLVVREGEQAYVERQGSVRVLRVPTHEADLKS
                     QIQSYQRALKRQLDGADYDVVHCRDSWSCLPVLEARSRLGFAVVYDMTRSPLGESVMD
                     PELDAQYGRDEEAAILAADLVLAPTPAAVKALQARGKPDRVVFSPPGVDVDRFDWDEP
                     PKAGTPPKILYTGSIDPGRGVRVLVRAMAAIVREVDARLVLAGPMVAPKFDTALRDGI
                     RELGLQDKVDILGPVDHDQLPALIATATVCVVPAASDLTPNPAVVYPTKLLEYMACKR
                     AIVAPKRETVQMVAENGREALLFEAGDPID"
     gene            1085..1339
                     /locus_tag="VMZ53_08345"
     CDS             1085..1339
                     /locus_tag="VMZ53_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28504.1"
                     /translation="MNRLIEYLRQNLMIDFQGDLSVSKVKELLAGDDSRDAKTLLSKL
                     VADKKVEDMLVVLCDCLTEPVQRALTDDVMREQLRIYSES"
     gene            1382..1666
                     /locus_tag="VMZ53_08350"
     CDS             1382..1666
                     /locus_tag="VMZ53_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28505.1"
                     /translation="MGTPEPDSKTPAFIAFGMIVLGLVIALIQGGITHGSITGGIIAA
                     LGAGPACFGMWKGIQQETQGTLAISVAAVLASLAIGGLLIVLRVVNWVAG"
     gene            1663..2679
                     /locus_tag="VMZ53_08355"
     CDS             1663..2679
                     /locus_tag="VMZ53_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HUS28506.1"
                     /translation="MKLALAALLLAATSVASAAPGKLTYTGKAMGTYVTVWFWTDKEP
                     EAAKAAEQVFSEMKRLDEKMTTWTADSEVSKVNAAAGTAKPVAVSDETFEVIERAQDI
                     SKKSGGVFDITVGSFKGLWKFDEDMDGTLPDPAEVKKRLKLVGYKELVLDKKKHTVSI
                     RKKGMSITLGGIAKGYAVDKCAALLKAAGFTDFMVQAGGDMYVAGKKGNEPWVVGIRD
                     PRGTDLFAGMPITDHSFSTSGDYERGFVKDGVRYHHILDPRTGQPAHASRSVTIRAKD
                     AYTADSWSKVMFIVGYQKGLELIKKYKLDDFEVVWVDDKNELHMTPAIEKELKIVKQP
                     TAGP"
     gene            2684..3139
                     /locus_tag="VMZ53_08360"
     CDS             2684..3139
                     /locus_tag="VMZ53_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HUS28507.1"
                     /translation="MRCPRDQSALHAVGKRLTCTTCHGVFVEREDLAELVRMMAPDLH
                     VDIHVPMQARSTEQAALACPMCTHAMQPVHLGVMPVDHCTMHGVWFDGGELQHVLRTL
                     GEGYADREETRNFSPPGGRRELQPAQWAGEETLGQTLERVITWIFRLRR"
     gene            complement(3158..>4009)
                     /locus_tag="VMZ53_08365"
     CDS             complement(3158..>4009)
                     /locus_tag="VMZ53_08365"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HUS28508.1"
                     /translation="GSSARKLKRGAANLLGGRAARFELFGMTAAELERDFDLDRMLNR
                     GYLPRMYQAERAGRLLSAYVADYLKEEIAAEGLVRNLPVFSRFLDVAALSDTDLVNAS
                     NIARDCKVSTPTVQSYFEILVDTLLGRWLPAYTKRPKRRVIQGPKFFFADVGVVNYLA
                     KRGAIARGGELYGKALENWVFHELTAAIAYRESDTALSYWRLASGIEVDFVLDDVRVA
                     IEVKASANITGDHLKGLRAIAQDHPRVARRVVVCLEPRRRKTDEGIEILPIDAFVKEL
                     ASGSLFA"
BASE COUNT          744 a         1338 c         1267 g          660 t
ORIGIN      
        1 aagtcgatcg gatcgccggc ctcgaacagc agcgcctcgc gcccgttctc cgcgaccatc
       61 tggacggtct cgcgcttggg cgcgacgatc gcgcgcttgc acgccatgta ctcgagcagc
      121 ttcgtcggat agacgaccgc cggattcggc gtgaggtcac tcgccgcggg cacgacgcag
      181 accgtcgccg tcgcgatcag cgccggaagc tggtcgtgat cgacgggccc gaggatgtcg
      241 accttgtcct gcaggccgag ctcgcggatg ccatcgcgca gcgcggtatc gaatttgggc
      301 gccaccatcg gcccggcgag cacgagtcgc gcatcgacct cgcggacgat cgccgccatc
      361 gcacgcacga gcacgcgcac gccgcgacct ggatcgatcg agcccgtgta caggatcttc
      421 ggcggcgtac cggccttcgg cggctcgtcc caatcgaagc gatcgacgtc gacgccaggc
      481 ggcgagaaca cgacgcgatc cggcttgccg cgcgcctgca gcgccttcac agccgcgggc
      541 gtcggtgcga gcacgagatc cgctgcgagg atcgccgcct cttcatcgcg gccatactgc
      601 gcgtccagct ccggatccat cacgctctcg ccgagcggcg accgcgtcat gtcgtagacg
      661 accgcaaaac cgagccgcga cctcgcctcg agcacgggca agcagctcca gctatcgcgg
      721 cagtggacga cgtcataatc cgcgccgtcg agctgccgct tcagggcgcg ctggtacgac
      781 tggatctgcg acttgaggtc ggcttcgtgc gtcggcacgc ggaggacgcg caccgaaccc
      841 tgccgctcca cgtacgcctg ctcgccctca cgcacgacta acaagtcgac ggcatgaaac
      901 ggcgtcaacg cgcgaatcac gtgtcttagc tggacaccgg cccggcgagg ccccggtaca
      961 gcgcagaatc ccgtcaaaag cacacggggc atcgcagtct tatcgtcggg tacttcgcaa
     1021 actcacgcaa gacctctcgc cgcgtcgcct tgatccactc gtccgggtgc gttatctccg
     1081 cagcgtgaat cgcctgatcg agtacctccg acaaaatttg atgatcgatt ttcagggtga
     1141 tctctcggtg tcgaaggtga aggagctgct ggcgggggac gacagccggg acgcgaagac
     1201 cctgctctcc aagcttgtcg ccgacaagaa agtcgaagac atgctcgtag tcctctgcga
     1261 ctgcctcacc gagcccgtgc agcgcgcact caccgacgac gtgatgcgcg agcagctgcg
     1321 catatattcg gagagttagt tccggcaggt agcaggcgcc gcagtctgct acgttcccgt
     1381 catggggaca ccagagcctg attcgaagac gcctgcgttc atcgcctttg gcatgatcgt
     1441 gctcggcctc gtgatcgcct tgattcaggg cggcatcacg catggctcga tcacgggcgg
     1501 catcatcgct gcgctgggcg ctggccccgc atgcttcggc atgtggaaag gcatccagca
     1561 agagacgcag ggcacgctcg ccatctcggt cgccgcggta ctcgcatcgc tcgcgatcgg
     1621 cggcctcttg atcgtactgc gcgtcgtcaa ctgggtcgct ggatgaagct cgcgctcgcc
     1681 gccctgctgc tcgcagctac ctcggtcgcg tccgccgcgc cgggcaagct gacgtacacg
     1741 ggcaaggcga tgggcaccta cgtcacggtc tggttctgga ccgacaagga gcccgaggcg
     1801 gcgaaggccg ccgaacaagt cttctccgag atgaagcgtc tcgacgagaa gatgacgacg
     1861 tggaccgccg actccgaagt ctccaaggtc aacgccgccg ccggcaccgc caagcccgtc
     1921 gcggtctccg acgagacgtt cgaggtgatc gagcgcgcgc aggacatctc caaaaaatcc
     1981 ggcggcgtct tcgacatcac cgtcggctcg ttcaaaggcc tctggaagtt cgacgaggac
     2041 atggacggca ccctgcccga cccggccgag gtcaaaaagc gcctcaagct cgtcggctac
     2101 aaagagcttg tcctcgacaa gaagaagcac accgtctcga tccgcaagaa gggcatgtcg
     2161 atcacgctcg gcggtatcgc gaagggctac gcggtcgaca aatgcgccgc gctcctcaag
     2221 gccgctggct tcaccgactt catggtgcag gcaggcggcg atatgtacgt cgccggcaag
     2281 aaaggcaacg agccgtgggt cgtcggcatt cgcgatccgc gaggcacgga cctgttcgcg
     2341 ggcatgccga tcaccgatca ctcgttctcg acgtcaggcg actacgagcg cggcttcgtc
     2401 aaagacggcg tccgctatca ccacatcctc gatccacgca cgggccaacc cgcgcacgcg
     2461 tcgcgctcgg tgacgatccg cgcaaaagac gcgtacaccg ccgactcgtg gagcaaggtg
     2521 atgttcatcg tcggctatca aaagggcctc gagctgatca agaagtacaa gctcgatgac
     2581 ttcgaggtcg tgtgggtcga cgacaagaac gagctacaca tgacgccggc gatcgaaaaa
     2641 gagctgaaga tcgtcaagca gccgaccgct ggtccctaag cgcgtgcgct gcccacgcga
     2701 ccaaagcgcg ctgcacgccg tcggtaagcg actcacgtgc acgacgtgcc acggcgtgtt
     2761 cgtcgagcgc gaagacctcg cggagctcgt gcgcatgatg gcgccagacc tccacgtcga
     2821 catccacgtc cccatgcaag ctcgctcgac cgagcaggcc gccctcgcct gtccgatgtg
     2881 cacgcacgcg atgcagccgg tccacctcgg cgtgatgccc gtcgatcact gcacgatgca
     2941 cggtgtctgg ttcgacggtg gcgagctgca acacgtgctg cgaaccctcg gcgagggcta
     3001 cgcggaccgc gaagagacgc gcaatttctc gccgcccggt ggtcgccgcg agctccagcc
     3061 cgcgcaatgg gctggcgaag agacactcgg ccaaaccctc gagcgcgtga tcacctggat
     3121 cttccgtctg cggcgctgag cggacgctcg tcggttctca agcgaacaac gagccgctcg
     3181 ctagctcttt gacgaacgcg tcgatcggca gaatctcgat gccttcgtcg gtcttgcgac
     3241 gccgcggttc gagacacacg acgacccgcc gcgcgacgcg cgggtgatcc tgcgcgatcg
     3301 cccgcaggcc cttgaggtgg tcgcccgtga tgttcgcgct tgccttgacc tcgatcgcga
     3361 cccggacgtc atcgaggacg aagtcgactt cgatgccgct ggcgaggcgc cagtacgaga
     3421 gcgcggtgtc cgactcgcgg tatgcaatcg cggccgtcag ctcgtgaaac acccagttct
     3481 cgagcgcctt gccgtagagc tcgcctccgc gcgcgattgc tccgcgcttc gcgaggtagt
     3541 tgacgacacc gacatcggcg aagaagaact ttggtccttg aatgacgcgc cgcttgggcc
     3601 gcttcgtgta cgcgggcaac caacggccga gcaacgtatc gacgaggatc tcgaagtacg
     3661 actggaccgt cggcgtggag accttgcagt cacgagcgat gttcgatgcg ttgacgaggt
     3721 cggtgtccga tagtgctgcg acgtcgagaa agcgcgagaa cacgggcagg ttccgcacga
     3781 gaccttcggc cgcgatttcc tccttgaggt aatcggcgac gtacgcggag agcagtcggc
     3841 cggcgcgctc ggcctggtac atgcgcggca ggtagccacg attgagcatg cgatcgaggt
     3901 cgaagtctcg ctcgagctcg gccgcggtca tcccgaacag ctcgaagcgg gctgcgcggc
     3961 cgccgagcag gtttgcagca cctcgcttga gcttccgtgc actcgagcc
//
LOCUS       DATLBZ010000158         5738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10509829, whole genome shotgun sequence.
ACCESSION   DATLBZ010000158 DATLBZ010000000
VERSION     DATLBZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10509829"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VMZ53_08370"
     CDS             <1..322
                     /locus_tag="VMZ53_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28509.1"
                     /translation="YEGRIVDADDSGALWLVEEMPSGRAIVVAEPVQGEASLVLPHVS
                     RATGFFASAGSLYWQEGDELLTAPRAGGAASIVASLPGAAGAVADGYVYFVNGSAIER
                     LPVE"
     gene            complement(385..585)
                     /locus_tag="VMZ53_08375"
     CDS             complement(385..585)
                     /locus_tag="VMZ53_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HUS28510.1"
                     /translation="MAEGTIKRLTDKGFGFISNSSGKDLFFHMSAVEGTRFEELREGQ
                     KVSYNEGTGPKGARAENVRVIG"
     gene            767..1426
                     /locus_tag="VMZ53_08380"
     CDS             767..1426
                     /locus_tag="VMZ53_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28511.1"
                     /translation="MSGTWETLATVQTDEGPLELRRRRDDWLMVIAGRVLMNSFSRSS
                     EEELSRLAVEKLGPRPAPRVLVSGLGMGFTLRALLDRVPETARVTVAEINPVVVEWCG
                     GVLGPATDDAIRDPRVRVHVGDVAALIGGSAAGAFDLILLDLYEGPNSSTQEPDDPFY
                     GPAALARAHRALTPKGVLGIWSEDADAPFARRFAAGRFSVTKHSIGRGGRRHFVYLGQ
                     K"
     gene            complement(1427..2074)
                     /locus_tag="VMZ53_08385"
     CDS             complement(1427..2074)
                     /locus_tag="VMZ53_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS28512.1"
                     /translation="MKARQLVKRDLVKPRSRFHHGQLREAVVAATLAIVERDGHQAVS
                     LRAIAHALGVTEPAIYRHFENKEAILAEVSAHGMRGFMGTVTEALNSTPEPIDAVEAT
                     ARAYVRYAYAHPGWFRLCFSRIGTEQLSKHHVVQTAMVEAGAAYMGKMFATLGRFIPQ
                     ARVPDAYRAFWALAHGLSSFVVERVFQLVETDEERIAAADAAIGVFVDAYRARAA"
     gene            2090..2653
                     /locus_tag="VMZ53_08390"
     CDS             2090..2653
                     /locus_tag="VMZ53_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUS28513.1"
                     /translation="MDGVTPYTADSPPTGEHFLFRTSARGPTKKFGFQWTLKAGKRGP
                     GEHMHPLETETFSIVSGVLRIWIEGVPRDYHAGETVAIPPNTRHRFLNPGKEPVVVDV
                     TLDGTEMEDVFMPFVTEVGRGKKPTMRGFVRWIVHMGQHPKASVPTSRIARGIGAAVV
                     GLCRLFGARAYPCPAPAWEDERVKAAA"
     gene            complement(2650..3669)
                     /locus_tag="VMZ53_08395"
     CDS             complement(2650..3669)
                     /locus_tag="VMZ53_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28514.1"
                     /translation="MVPGFFGFVNFGRLVYFGHVREYLEDAFARERLRVEIHGVRPGP
                     TSSLSKRSAELLRTITESAPADAPIHLIGHSTGGLDARLLVSPNVTLTTDLDVAAVAS
                     RVRSVVAVASPHRGTPLAAFFSSLLGQQLLRLLSLATITVLRRGRLPAAVFTHAARVL
                     AKVAIQRTSASVALLDHLERELVGNIEPEQHDLIDGFLQNIHGDQDLMPQLTPAAMDL
                     FQAATSDRPGVRYASVTACAAPPSVRARLKLGVHPWQQSTYALYAWLHRRVGEGDGMV
                     PIDSQSHGEVLHKARADHLDVLGHFEDEDAKPPHVDWLNSGSGFDRPQFEALWTSVAR
                     FVARR"
     gene            complement(3706..4623)
                     /locus_tag="VMZ53_08400"
     CDS             complement(3706..4623)
                     /locus_tag="VMZ53_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUS28515.1"
                     /translation="MRWVVVLVLMLASVAQAQPKTNVDAVDSDGWTALMRAAQGGKTV
                     EVANLLDRGANIEASNPKVYDGATPLVLALEYGQDDTAKLLLDRGASVAGKLGTSALV
                     LAARGGFDDIVDRLVTAKVSPKGTLALQAAARYGHVSTIKRLVKAGAVVRDKDTGDHD
                     YTPFIVACQENQLAAARALLDAGANVNDVDADGTPALHWAVFGERPDEVHEYREMGKP
                     HDTYWIPHKDAPLVKLLLGKGAKREAVDADGNTALHKAAMLDVAAAAKVLLAAGANRA
                     KKNAEGQTAYELAKDRKNSVEPILRPKKQ"
     gene            complement(4644..5567)
                     /locus_tag="VMZ53_08405"
     CDS             complement(4644..5567)
                     /locus_tag="VMZ53_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28516.1"
                     /translation="MSHDILDWIAREVGARAARRRERIQSLWGGVGELFRIELEDGPI
                     DTAIVKWAQPPVGVDDVSTRRKKMSFAVEVAFYRSFARRCDDSCRVANLLAARISEED
                     AVLVLEDLDAAGYPRRTDEADDGQLDAALAWLASFHARFVGERPGQLWPTGTYWHLDT
                     RLDELAVIEDRALREAAPAIAYKLSSARFQTIVHGDPKEANFCFGPAGIAAVDFQYTG
                     RACGMTDLAYLLYGRADEPADGIDHVRIATYFAHLRARLPATVDGDALEAEWRALYAY
                     ARLDFCRFLAGWRPAMWERDTRGRRFVQHNL"
BASE COUNT          986 a         1843 c         1928 g          981 t
ORIGIN      
        1 gtacgaaggc cgcatcgtcg acgccgatga cagcggcgcg ctgtggctcg tcgaagaaat
       61 gccgtcgggc cgagccatcg tcgtcgccga gccggtgcaa ggcgaggcat cgctcgtgct
      121 cccgcacgtc tcgcgagcga ccggattctt tgcgtcggcc ggctcgctgt actggcaaga
      181 gggcgacgag ctgctcaccg cgcctcgggc cggcggcgcg gcatcgatcg tcgcatcgct
      241 gcccggagcg gcgggcgctg ttgcggacgg ctacgtctac ttcgtgaacg gctcggcgat
      301 cgagcgactg cccgtcgagt agatccgagc ccgaaaacgc aaagagccgg cacgaggccg
      361 gcccttcttg gttagttgag ttggttagcc gataacgcga acgttctcag cacgcgcgcc
      421 cttggggccg gttccttcgt tgtagctgac cttctggcct tcgcggagct cttcaaagcg
      481 agtcccctcg accgcggaca tgtgaaagaa cagatccttg ccggagctgt tcgagatgaa
      541 accaaaaccc ttgtcggtca aacgcttgat cgtaccttca gccatcgtag ctctcctcgt
      601 aacatcgcga tgcagacctc gtggaccaag aggggccacg cagcggcgct gcatctgctg
      661 catatgtggg cttttttcga cggaaagtcg acccgaatcg acctaaatgt cgaagatgtg
      721 acgtccaagg ctgcaaattg cgcgtttgcg ctagggtgaa acgctgatgt cgggcacctg
      781 ggagacgctc gcgaccgtcc aaacggacga gggcccactc gagctgcgca ggcgccgaga
      841 cgactggctg atggtgattg cgggccgcgt gctgatgaac agcttctcgc gcagctcgga
      901 ggaagagctt tcgcgcctcg cggtggagaa gctcggcccc cgccctgctc cgcgcgtgct
      961 cgtcagcggc ctcggcatgg gcttcacgct gcgggcgctg ctcgatcgcg tgcccgaaac
     1021 cgcgcgcgtg accgtcgccg agatcaatcc ggtggtggtc gagtggtgcg gcggcgtgct
     1081 cgggcccgcc acggacgatg cgatccgcga tccgcgcgtg cgggtgcacg tcggtgatgt
     1141 tgccgcgctc atcggcggct cggcagcagg cgcgttcgac ctgatcttgc tcgatctata
     1201 cgagggtcca aacagctcga cgcaggaacc cgacgatccg ttctacggtc ccgcggctct
     1261 cgcgcgagcg catcgcgcgc tcactccgaa gggggtgctc ggcatctggt ccgaagatgc
     1321 agatgcgccg ttcgcgcgtc gcttcgccgc cggccgcttc agcgtgacga agcactcgat
     1381 cggacgcggc ggacgtcgac acttcgtgta tctcggccag aagtagtcag gcggcacgcg
     1441 cgcgatacgc atcgacgaat acgccgatcg ccgcgtcggc cgccgcaatg cgttcctcat
     1501 ctgtctcgac aagctggaat acacgctcga cgacgaacga ggagaggccg tgagcgagcg
     1561 cccagaacgc gcgataggcg tcggggactc tcgcctgcgg gatgaaccgt ccgagcgtcg
     1621 cgaacatctt gcccatgtac gcagcacctg cttcgaccat cgcggtctgc acgacgtgat
     1681 gcttgctgag ctgctcggtg ccgatgcgcg agaagcacag ccggaaccag ccgggatgtg
     1741 cgtaggcgta gcgcacgtac gcgcgcgcag tcgcttcgac ggcatcgatc ggctcgggcg
     1801 tcgagttgag cgcctcggtg acggtgccca tgaagccgcg catcccgtgg gccgagacct
     1861 cggcgaggat cgcttctttg ttctcgaagt ggcgatagat cgcgggctcg gtgacgccga
     1921 gcgcatgcgc gatcgcccgg agcgacaccg cttggtggcc gtctctctcg acgatggcga
     1981 gggtcgcagc aacaacggct tcgcgtaact ggccgtgatg aaaccgcgag cgcggcttga
     2041 ccagatcgcg cttgacgagc tgcctggcct tcatgttatc aatgataaca tggatggtgt
     2101 gacaccgtat acagctgact ctcctccaac tggagaacac ttcctgttcc ggacgtccgc
     2161 gcgcgggccg acgaagaagt tcggctttca gtggacgctc aaagccggca agcgcggccc
     2221 gggcgagcac atgcacccgc tagagacaga gacgttctcg atcgtcagcg gcgtgcttcg
     2281 catctggatc gagggcgtac cgcgcgacta ccacgcgggc gagaccgtcg cgattccgcc
     2341 gaatacgcgg catcgctttc tcaatcccgg taaagagccc gtcgtcgtcg acgtcacgct
     2401 cgatgggacc gagatggaag acgtgttcat gccgttcgtc accgaggtcg gtcgcggcaa
     2461 gaagccgacc atgcgcgggt tcgtgcgctg gatcgtgcac atggggcaac acccgaaggc
     2521 gagcgtgccg acatcgcgaa tcgcacgcgg catcggcgct gcggtcgtcg gcctgtgccg
     2581 cctgttcggt gcgcgcgcgt atccgtgccc cgcgccggca tgggaagacg agcgcgtgaa
     2641 ggccgctgct tagcgacgcg cgacgaaccg cgcgacactc gtccacaacg cttcgaactg
     2701 cgggcgatca aaccccgagc cgctgttgag ccaatcgacg tgcggcggtt tcgcgtcttc
     2761 gtcctcgaag tgaccgagga cgtcgagatg atcggcacgc gccttgtgga gcacttcgcc
     2821 gtgcgactgc gaatcgatcg gcaccatgcc gtcgccctcg cccactcgcc ggtgcagcca
     2881 cgcgtacagc gcgtacgtcg attgctgcca ggggtgcacg ccgagcttga gccgcgcacg
     2941 aacgctcgga ggagcggcgc acgcggtaac ggacgcgtag cgaacgcccg gccggtccga
     3001 cgtcgcagct tgaaatagat ccatcgcagc cggcgtgagc tgcggcatga ggtcctgatc
     3061 gccgtgaatg ttctggagga agccatcgat gagatcgtgc tgctcgggct cgatgttgcc
     3121 gacgagctcg cgctcgagat gatcgagcaa cgccaccgac gcgctcgtgc gctggatcgc
     3181 gactttggcg agcacgcggg ccgcgtgcgt gaacacggca gcggggagcc ggcctcgccg
     3241 aagcacggtg atcgtcgcga gcgagagcaa tcgcagcagc tgctgtccga gcaggctcga
     3301 gaagaatgcg gcgagcggcg tcccgcgatg agggctcgca acggcgacga cggagcggac
     3361 acggctcgca acggcggcca cgtcgagatc ggtcgtgagc gtcacgttcg gcgagacgag
     3421 cagccgcgcg tcgagaccac cggtcgaatg gccgatgagg tgaatcggcg catcggcagg
     3481 tgcggactcg gtgatcgtgc ggagcagctc ggcggatcgc ttgctcagcg atgacgtcgg
     3541 tcccgggcga acgccgtgga tctcgacgcg caaacgctcg cgtgcgaacg cgtcctcgag
     3601 gtactcgcga acgtggccga agtacacgag gcggccgaag ttcacgaagc cgaagaagcc
     3661 tggaaccaag aagacgtggt gcgtgacgtt gcgcaggcta cgagcttact gcttcttcgg
     3721 acggaggatc ggctcgacgg aattcttgcg atccttcgcg agctcgtagg ccgtctggcc
     3781 ctcggcgttc ttcttcgcgc ggttcgcacc ggctgcgagg agcaccttgg ctgctgccgc
     3841 tacatcgagc atcgcggctt tgtgcagcgc ggtgttgcca tcggcgtcga cggcttcgcg
     3901 cttcgcgccc ttgccgagca gcagcttcac gagtggcgcg tctttgtgcg ggatccagta
     3961 ggtgtcgtgc ggcttgccca tctcgcgata ctcgtgaact tcgtctggac gctcgccgaa
     4021 aaccgcccag tgcagcgccg gcgtaccatc cgcgtcgaca tcgttgacgt tcgcaccggc
     4081 gtcgaggagc gcgcgcgcag cagcgagctg gttctcctgg cacgcgacga tgaacggcgt
     4141 gtagtcgtga tcgccggtgt ccttgtcgcg aaccaccgca ccggctttga cgagccgctt
     4201 gatcgtcgag acgtgtccgt accgagcagc ggcctgcagt gcgagcgtcc ctttcggaga
     4261 caccttcgcg gtgacgaggc gatcgacgat gtcgtcgaag ccaccgcggg cggcgagcac
     4321 gagcgcagac gtgccgagct tgccagcgac gctggcgccg cgatcgagga gcagctttgc
     4381 agtgtcgtcc tgcccgtact cgagcgcgag cacgagcggc gtcgcgccgt cgtacacctt
     4441 cgggttgctc gcctcgatgt tcgcgccccg atcgagcaga ttcgcgacct cgacggtctt
     4501 gccgccttgt gcagcgcgca tcagcgctgt ccaaccatcg ctgtcgaccg cgtccacgtt
     4561 cgtcttcggc tgcgcctgtg cgacggacgc gagcatcagc acgagcacga cgacccaccg
     4621 catgctcgtg acactagcag cgattataga ttgtgctgca cgaatcgccg accgcgcgta
     4681 tcgcgctccc acatcgcggg gcgccagcca gcgaggaagc gacagaaatc gaggcgagcg
     4741 taggcataga gcgcgcgcca ctccgcctcg agcgcatcgc catcgacggt cgcgggaagg
     4801 cgcgctcgca gatgcgcgaa gtacgtcgcg atgcgcacat gatcgatgcc atcggcgggc
     4861 tcgtcagcgc ggccatataa gagatacgca aggtcggtca ttccgcacgc acggccggtg
     4921 tactggaagt cgaccgcggc gatacccgcg ggtccgaagc agaagttcgc ctccttcgga
     4981 tcgccgtgaa cgatcgtctg gaatcgcgcc gacgacagct tgtacgcgat tgcgggcgcg
     5041 gcctcgcgaa gtgcccggtc ctcgatgacc gcgagctcgt cgaggcgcgt gtcgagatgc
     5101 cagtacgttc ccgtcggcca cagctgaccg ggacgctcgc cgacgaagcg cgcgtgaaag
     5161 ctcgcaagcc atgcgagagc agcgtcgagc tgcccgtcgt ccgcttcgtc cgtgcgcctc
     5221 ggatagccgg cagcatcgag atcttcgagg accaacaccg cgtcttcctc ggagatccga
     5281 gcggcgagca gattcgcgac gcgacagcta tcgtcgcagc gacgcgcgaa cgagcggtag
     5341 aacgcgacct cgacggcgaa cgacatcttc ttgcgccgcg tggacacgtc gtcgacacca
     5401 accggtggct gcgcccactt cacgatcgcg gtgtcgatgg gaccgtcttc gagctcgatg
     5461 cggaacagct caccgacgcc gccccacagt gattggatac gctcacgtcg ccgggccgca
     5521 cgtgcgccca cctcgcgcgc gatccagtcg aggatgtcgt gagacactgt gcacagtatg
     5581 cgcgtcgggg agctgttgct tcaacgcggt ttggtggact gggagccact cgcgctcgca
     5641 ttgggtgacc aggcgggtag cggcatgcgg ctgtgctcgc tcctcgtgtc gcgtggcctg
     5701 ctcgatttcg acgaagcctc gcgtctgctc ggtgaagt
//
LOCUS       DATLBZ010000159        11916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2387466, whole genome shotgun sequence.
ACCESSION   DATLBZ010000159 DATLBZ010000000
VERSION     DATLBZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11916
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2387466"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..708)
                     /locus_tag="VMZ53_08410"
     CDS             complement(19..708)
                     /locus_tag="VMZ53_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase A"
                     /protein_id="HUS28517.1"
                     /translation="MSSDELDRFLANARGTAATAKIYSLDELRERRGDVAQASWGLAA
                     LRGRLVELSARGATGTLTAAIELVAEAQEQSEPVLWLTLQSGTFYPPDVAQSGIDLEA
                     LVVVRCQDATTAARSAERCLRSGAFGLVILDLGHSESRRGDDRDLSMQVQGRLVTLAQ
                     THDAAVVCLTEKSEDTASLGSLVSLRAEALRRREPPTSQFSVEVRVLKDKRRGPGFTK
                     LLKRSGPAGLT"
     gene            complement(787..1620)
                     /locus_tag="VMZ53_08415"
     CDS             complement(787..1620)
                     /locus_tag="VMZ53_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUS28518.1"
                     /translation="MVTAVVVMRRGHSAEPAPVVAKELEKPALPPIIDATVLATDVIK
                     LQRNAVEPVVDNGQTLGVKVKDPELARVLGLDTDDVIVSLSGRVLTRDMDIYDAVFNV
                     SMMSGTTMYAEITRAGTPTLLRWKLDGDLRQARYANAGNTLGGLYGGTYTPPPLPPPD
                     PILDTIEKVDDTHVKMPRKTAEHIFSNPMATMKGARVVPAVKNGQPSGIKLYAIRPSS
                     VFAKLGFANGDTIEDINGVAITSADKALELYTKLPKLDQVKVTLTRRGNPMQLTITIT
                     K"
     gene            1742..2152
                     /locus_tag="VMZ53_08420"
     CDS             1742..2152
                     /locus_tag="VMZ53_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28519.1"
                     /translation="MSVAVTLDMDKSRNREDAPGLQAPDYFSLVIEWESDADELSAAP
                     GEELDEEQTNPVPHWDVVDEASADSFPASDPPAWMGSSGHAAPTEASAAKVEPHVQAI
                     SEHRVRKLATKIALGVAAMGMGVHMIHRIRRNAA"
     gene            2221..2748
                     /locus_tag="VMZ53_08425"
     CDS             2221..2748
                     /locus_tag="VMZ53_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HUS28520.1"
                     /translation="MARIAFVVGEDFEDSEFKVPYDELRAAGHDVEVLGAKAGETMKG
                     KKGKEEIAIAAATSERDAQSYDAIVIPGGYSPDHLRMSKDVVKFVSSAVRAGKVIAAV
                     CHGPQLLIEADAVENKKLTSWPSVRTDLLNAGAKWVDREVVVDGKLITSRKPDDLPAF
                     IAAIREQLDKAKIAA"
     gene            2745..3563
                     /locus_tag="VMZ53_08430"
     CDS             2745..3563
                     /locus_tag="VMZ53_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28521.1"
                     /translation="MKYDAALAELYQAPLEQFIAERKRLVGELKAAGDKTGARVLGTR
                     AKPPVSAWVTNQLWWHARDAFDDLMKSADKLRKGQLAASGEHRDAIAKLRKRATAMLE
                     QAGHGATESTLRRVATTLSAIAASGTWDPDEPGTLSTDRDPPGFEAVGIPSGPIDAPP
                     PKPEPKHEKPHKPAKHDKHAEKSSKDELEELRERKRAEEQAEKKRAEDEKKRINAEKH
                     RLESALRTAKAELHDRERDAKQLEKQLHAATEKVSDAQEVVDEIEKQLGELRGS"
     gene            complement(3566..4909)
                     /locus_tag="VMZ53_08435"
     CDS             complement(3566..4909)
                     /locus_tag="VMZ53_08435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HUS28522.1"
                     /translation="MFENGVMQNKLMTLLALVGAAACGDDDDKPLTPDASVMVDAAVD
                     APPQVACTPQALRTDLTWYGNNRADLGTWLASKGCTSAGYNPAKKPVALFDWDNTVSK
                     NDFGDAFTFYLVSHDKIRQPPNQDWKQTSAYLTDAAATALSTACGTTVAAGDPLPTTT
                     NTACADQILNIYIASTVEGTTTGTAAFAGHNPRQIEPSYAWTAQLAAGYTHAEIKQFA
                     TDAITPQLAAAEGTTQSIASRTGSTALNGWLRIYDQSKDLIAVAKSQGYDVWVITASP
                     QDVIEAFAPMAGVPVDHVIGIHSKTDSAGKLTYKFEGCGTVPDDNQSLISYIQGKRCW
                     VNKIVYGDTTATAMQRRPDGQRQVFAAGDSDTDIEFLRDATYRLVINRAKTELMCHAY
                     NNANDGWRINPMFILPRSMKTSAFTCSTTCMNGLGVPCLDDANGVIANQVDAVHP"
     gene            complement(4974..7775)
                     /locus_tag="VMZ53_08440"
     CDS             complement(4974..7775)
                     /locus_tag="VMZ53_08440"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUS28523.1"
                     /translation="MKRHLLLVIVAACCPPEAAKPKITTVTPPAPDTKELAAPGETHL
                     KNVRQLTFGGDNAEAYWSFSGDRLIFQTNHQPHKCDQIEVMPSTGGPAKLVSTGKGRT
                     TCSYFLKGDQEIIYASTHEVSAECPTPPDMSKGYLWGLFDYDIYKAAPDGSNLRKLAP
                     APGYDAEATVCGKDGSIVFTSMRSGDLELWRMDADGGNLRQLTNLPGYDGGAFFSADC
                     SKLVWRSSRPQGNDLDAYKQLLAQNLVKPTKMDIWVANGDGTEAHQVTYLPGASFAPF
                     FFPDGKRILFSSNFKNPRGPEFDIYAIDIDGTHLEQITNAPGFDGFPVFSPDGTKLAF
                     SSNRQDVVGGKYRATGAPAGEHDTNVFVAEWVNSPPPTGAVETVSADRYRDAIYRLAD
                     DATEGRGIGTKGLRDAADFVQQQLAAAGAEPGYNGAWRQTFDVTTEVKRGASTALELD
                     GKAVAPEMFAPMPFSASKTVSAAVVEAGWGIVDAETKLDDYKGKNVKGKFVLVHRFVP
                     SVDGLGPTSSARLGDIRYKAFTARGKGAVGLIVVDDGDPKQDEAPLPSLATDGMADLV
                     SGGADAGLPIIVLKRDAAAGLAKGNHKVKLTVALEPVRTPTDNVVGLIKAGAANKLPG
                     VIVIGAHVDHLGMGGGSNALDPKVHAVHNGADDNASGVAALLEVAAALGTKRAELQRD
                     VYVVAFSGEEEGDLGSAQFVRNPPTKEPVVAMLNMDMVGRMRNNQLHVNGAESAKEWH
                     TLVDPACDAARVVCTVGGSGYGPSDHMAFYVAGIPVLFFFTGNHLDYHTATDDADKIN
                     SVGGARVAMIVTDIAMQLAKRADALTFVKAPPTTMQGDVRRVGASLGTVPSYSEDPNQ
                     PPGVILADVVPDGAAAKAGMKGGDRITQIGTVEIRNINDLMFVLQTAKPGTDVKVTFV
                     RDGKHQTVTATYGVPRGRK"
     gene            7804..8991
                     /gene="glp"
                     /locus_tag="VMZ53_08445"
     CDS             7804..8991
                     /gene="glp"
                     /locus_tag="VMZ53_08445"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HUS28524.1"
                     /translation="MLSVEEAAKTVASRIARLPVENVSLHDARNRILAADVVATRNLP
                     GFDNSAMDGFAARSAELPATLPVVAKVAAGQVLTDLIPERVAIRIMTGAPMPKGLDTV
                     VIQEDARIDGSNVTLPAAPAGDNVRKAGEDIAIGEIAVRAGERLDAAELGLLAALGVV
                     EVPVSRSPRVAIIATGDELVAAGSALGPGQIVDSSAHTLAALIPTCCGVVSYIGIAKD
                     DPAAMTALIASAMDHDVVITTGGVSVGDRDHVRGALKAAGVELELWKVAMKPGKPFSF
                     GVSGRVPVFGLPGNPVSSYVAFELFVRPALLAMQGATLTKRPRAPVSLARGYRKQAGR
                     THYLRAKLVRNGEQLVAHPHPKQGSAMLTSLVGCNGLVELPADATEVLPNTIVPAILL
                     EAV"
     gene            8988..9887
                     /locus_tag="VMZ53_08450"
     CDS             8988..9887
                     /locus_tag="VMZ53_08450"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUS28525.1"
                     /translation="MSSVNITVRVEDEGRIDKALANHYPNAGRRQLSELFDEGQVKLR
                     GKRAKKGDRVAAGDVIELLRAPVSGTDLQPVADPEVELDLLLERPELVVVNKPAGIPS
                     QPLRAGERATIANGIAARFPECITIGDDPRDGGLVHRLDIGTSGALVVARTAEAYRTL
                     RDAFGQGLVEKHYLAITDNRPVSRECDAPLVQRGDHVRVDHTDGLAAYTSFTVEKASE
                     SHALVRCVAQTGRMHQVRAHLAHVGSPIAGDTLYKGKPLGDDAGFYLHAALIVFPFGA
                     EKIRVEAPLPERFTAALAAAGLG"
     gene            complement(9903..11129)
                     /locus_tag="VMZ53_08455"
     CDS             complement(9903..11129)
                     /locus_tag="VMZ53_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS28526.1"
                     /translation="MIEADGTDDLPPTPIVMYLLGSNTEVADTLANMLRARPEGMPLL
                     VIGKNPVHAAKPDIWLPAEPPAPLLGAILTTMIGAETKSPWRRKGDMILGTSQHVRDL
                     LHTLDQLAPAQTAVLIQGESGVGKELVARALHFSSPRAAAPFVALNCGAIPETLLEAE
                     LFGYQRGAFTGAVATHVGAIESSENGTLFLDEIGEMPMAMQVRLLRVLQTNEVTRLGS
                     TEPRFINFRLVTATNRDLAAETKLGRFREDLYYRIHVYPIRVPPLRERPEDIPPIANH
                     YLSRIAERDKKPTLRLSSLALERMLAHSWPGNVRELVNTLERAAVMAGEDVIEGDHIM
                     LPTSATSGPTTASILPYKDAKQAFEKEYYSRLMRVAEGNVSLASKIGQKTRKEIYDAL
                     KRLGLETVEFRTEATD"
     gene            11474..>11916
                     /locus_tag="VMZ53_08460"
     CDS             11474..>11916
                     /locus_tag="VMZ53_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28527.1"
                     /translation="MGKTYVWMLALGVAGCTTDPEVTSTEEFLKRNNGFIPNGVQVPN
                     DHGSSSTYARSGKIDLNNEFFQDLGTNGRRCITCHLPTAGWGITPEQVQATFELTRGG
                     VKNDGFGLGAIFRTNDGANSPDYRADQLDTVAERRAAYSMLLNHGL"
BASE COUNT         2082 a         3888 c         3821 g         2125 t
ORIGIN      
        1 gcgcctcgcc tacctcgcct aggtcagtcc agccggacca ctgcgtttga gcagcttcgt
       61 gaacccgggc ccgcgccgtt tgtctttgag cacgcgcacc tcgactgaaa actgcgacgt
      121 gggtggctct cggcgacgca gcgcctcggc gcgcagagac acgagtgagc cgagcgacgc
      181 ggtgtcctcc gacttctcgg tcaggcatac gaccgcggca tcgtgcgttt gcgcgagcgt
      241 cacgaggcgg ccttggacct gcatcgacag gtcgcggtcg tcacctcggc gcgattcgct
      301 gtgaccgaga tcgaggatca cgaggccgaa ggcgccggag cgcaagcagc gttcggcgga
      361 gcgcgcggct gtcgtggcgt cctggcatcg gacgacgacg agcgcctcga gatcgatccc
      421 actctgtgcg acgtcgggcg gatagaacgt gccgctttgc agcgtcagcc agagcacggg
      481 ctcgctctgc tcttgcgctt ccgcgaccag ctcgatcgcg gcggtgagcg tgcccgttgc
      541 acctcgcgcg gacagctcga cgaggcgacc gcgaagtgcg gcgaggcccc agctcgcttg
      601 cgcgacatcg ccacgacgct cgcgaagctc gtcgagcgag tagatcttcg ccgtcgctgc
      661 ggtgccgcga gcattcgcga ggaatcgatc gagctcgtcg gacgacatct ggtcgtatgt
      721 tcagtatcag atccgtatga cacgccggca aggcgtcgct cgccactccc gccgatccag
      781 cggagatcac ttcgtgatcg tgatggtcag ctgcatcgga tttccccgac gggtcagcgt
      841 gaccttcact tgatcgagct tcggcagctt cgtgtagagc tcgagcgcct tgtccgcgct
      901 cgtgatcgcg acgccgttga tgtcttcgat cgtgtcgccg ttggcgaagc cgagcttggc
      961 gaacacggac gacggacgaa tcgcgtagag cttgatgcca gacggctggc cgttcttcac
     1021 cgccggcacg acgcgcgcgc ccttcatcgt cgccatcgga ttgctgaaga tgtgctcggc
     1081 cgtcttgcgc ggcatcttca cgtgcgtgtc gtcgaccttc tcgatcgtgt cgagaatcgg
     1141 atcgggcgga ggcagtggcg gtggtgtgta tgttccgccg tagagaccgc cgagcgtgtt
     1201 gcccgcattc gcgtagcgcg cttgccgaag atcgccatcg agcttccagc gcagcagcgt
     1261 gggcgtgccg gctcgcgtga tttcagcgta catcgtcgtg ccgctcatca tcgagacgtt
     1321 gaacacggcg tcgtagatat ccatgtcacg cgtgagcacg cgccccgaga gcgacacgat
     1381 cacgtcgtcg gtatcgagtc cgagcacgcg cgcgagctcc ggatccttca ccttcacgcc
     1441 gagcgtttgg ccattgtcga cgacaggctc gacggcgttg cgctgcagct tgatgacatc
     1501 cgtggccagc accgtcgcgt cgatgatcgg cggcagcgcc ggcttctcga gctccttcgc
     1561 gaccacaggc gcgggttccg cggagtgacc gcgtcgcatg acaacgacag ccgtgaccac
     1621 ggcgatgatc atcgctacgc ccgcgaaact gatcgcgatg agacgtaagc gacgatctcc
     1681 gtcagacatc ccccgagaat gcgatgttct cgccgagagt caacacgttg caaacgtcgg
     1741 catgtcggtt gctgtaactc tcgatatgga caagtcgcgc aaccgtgaag atgcccccgg
     1801 actgcaggcg ccggattact tctcgctcgt gatcgagtgg gagagcgatg ccgatgagct
     1861 gtcggcggcg cccggcgaag agctcgacga agaacagacg aatccagtgc cccactggga
     1921 cgtcgtcgac gaggcgagcg cagattcgtt tcccgcgagc gatcctcccg cgtggatggg
     1981 tagctcggga catgcagcgc ccaccgaggc gagtgcggcg aaggtcgagc cccacgtgca
     2041 ggcgatctca gagcatcgcg tacgcaagct cgcaacgaag atcgcgctcg gtgttgctgc
     2101 aatgggaatg ggcgtccaca tgatccatcg gatccggcgc aacgctgcgt aactctcgcg
     2161 caacggcgcc cacgtcgccg ggcaccgtgc gttctcgctc ggcgacaaga ggtactagtc
     2221 atggcgcgga tcgcatttgt cgtcggtgaa gatttcgaag acagcgaatt caaagttcct
     2281 tacgacgagc ttcgcgctgc aggccacgac gtcgaagttc tcggcgcgaa agccggcgag
     2341 acgatgaagg gcaagaaggg caaggaagag atcgcgatcg cagccgcgac ctccgagcgc
     2401 gatgcccaga gctacgacgc gatcgtgatc cccggcgggt attcgccgga tcacctgcgc
     2461 atgagcaagg acgtcgtgaa gttcgtgagc agcgctgtgc gtgcgggcaa ggtcatcgcg
     2521 gcggtgtgtc acgggccgca gctgctcatc gaggccgacg ccgtggagaa caagaagctc
     2581 acatcgtggc cgagcgtccg cacggatctc ctcaacgccg gcgccaagtg ggtcgacaga
     2641 gaagtcgtcg tggacggcaa gctcatcacc tcgcgcaagc ctgatgacct tcctgcgttc
     2701 atcgcagcga tccgcgagca gctcgacaag gctaagatcg ccgcgtgaaa tacgacgccg
     2761 cgctcgctga gctgtatcaa gccccgctcg aacagttcat cgcagagcgc aagcgtctcg
     2821 ttggcgagct gaaggcagcg ggcgacaaga cgggcgcgag ggtgctcggc acgcgtgcga
     2881 agccgccggt ctccgcatgg gtgacgaatc agctctggtg gcacgcgcgc gacgcattcg
     2941 acgacctaat gaagagcgcg gacaagctgc gcaaaggcca gctcgctgcg agtggcgagc
     3001 accgcgatgc gatcgccaag ctgcgcaagc gcgcgaccgc gatgctcgag caagccggcc
     3061 acggtgcgac cgaatcgaca ctgcgccgcg tcgccacgac gctctcggcg atcgccgcga
     3121 gtggcacatg ggatccagac gagcccggca cgctgtcaac cgatcgcgat ccgccgggct
     3181 tcgaagccgt cggcattccg tcgggcccca tcgatgcacc gccgccgaag cccgagccca
     3241 agcacgagaa gccgcacaag cccgcgaagc acgacaagca cgccgagaag tcgagtaagg
     3301 acgagctcga agagctgcgc gagcgcaagc gcgccgagga gcaagccgag aagaagcgcg
     3361 ccgaagacga gaagaagcgc atcaatgccg agaaacatcg gctcgaatct gcgctgcgca
     3421 ctgccaaggc cgagctccac gaccgcgagc gcgacgcgaa gcagctcgaa aaacaactgc
     3481 acgccgctac cgagaaggtg agcgacgcgc aggaagtggt cgatgagatc gagaagcagc
     3541 tcggcgagct tcgcggcagc tgaccttacg ggtgaaccgc gtcgacttga ttggcgatga
     3601 cgccgttcgc gtcgtcgaga cagggcacgc cgagaccgtt catgcaggtg gtcgagcatg
     3661 tgaacgccga cgtcttcatc gacctcggca agatgaacat cggattgatg cgccagccgt
     3721 cgttcgcgtt gttgtacgcg tggcacatga gctccgtctt ggcgcgattg atcacgaggc
     3781 ggtaggtcgc atcgcgaagg aactcgatgt cagtgtcgga gtcgcccgcc gcaaacactt
     3841 ggcgctggcc gtcggggcgc ctctgcatcg ccgtcgccgt cgtgtcgccg tagacgatct
     3901 tgttgaccca gcaacgcttg ccttggatgt acgagatcag cgactggtta tcgtcgggaa
     3961 cggtgccgca gccctcgaac ttgtacgtga gcttgcctgc gctgtcggtc ttgctgtgga
     4021 taccgatcac gtgatcgacc ggtacgccgg ccatcggcgc gaacgcctcg atcacatcct
     4081 gcggtgacgc cgtaatgacc cacacgtcat agccctgcga tttcgcgacc gcgatgaggt
     4141 ccttcgactg atcgtagatg cgcagccaac cattgagagc cgtcgagccg gtgcggctcg
     4201 cgatcgattg cgtcgtaccc tcggccgccg cgagctgcgg cgtgatcgcg tcggtcgcga
     4261 actgcttgat ctcggcgtgc gtgtagccgg cagcgagctg cgcggtccaa gcgtacgagg
     4321 gctcgatctg gcgcggattg tggccggcaa atgcggccgt acctgtcgtc gtgccttcga
     4381 cggtgctggc gatgtagatg ttgaggatct ggtcggcgca cgcggtgttc gtcgtcgttg
     4441 gcaagggatc acccgccgcc accgtcgtgc cgcacgccgt cgacagtgcc gtggccgcgg
     4501 cgtccgttag gtaggcgctc gtttgcttcc agtcttggtt cggcggctgg cgaatcttgt
     4561 cgtgcgagac gagatagaac gtgaacgcgt cgccgaagtc gttcttgctc accgtgttgt
     4621 cccagtcgaa cagcgcgact ggcttcttcg cagggttgta cccggcggag gtgcagccct
     4681 tcgaagcgag ccacgttccg aggtctgcgc ggttgttgcc gtaccacgtg agatcggtgc
     4741 gcagtgcctg cggcgtgcag gccacctgcg gcggcgcgtc aacggcggca tcgaccatga
     4801 cggacgcgtc aggggttagt ggtttgtcgt cgtcgtcacc acacgccgcg gcgccaacca
     4861 acgcgagaag agtcatgagc ttgttctgca tgactccgtt ctcgaacact tgcgggtgat
     4921 gaccccatgg cgcccgcgtg aagaacgcgt gatctcgcgt tcaaccaggg cggctatttg
     4981 cgaccgcgcg gaacgccgta cgtcgctgtg acggtctggt gtttgccgtc gcgcacgaac
     5041 gtcaccttca catcggtgcc cggtttcgcc gtttgcagca cgaacatcag gtcgttgatg
     5101 ttccggattt cgaccgtgcc gatctgagtg atgcgatcgc cgcccttcat cccggctttc
     5161 gctgctgcgc catcggggac gacatcggcg aggatcacgc ccggcggttg attcggatct
     5221 tcgctatacg acggcaccgt gccgagcgat gcaccgacgc gacgaacatc gccctgcatc
     5281 gtggtcggcg gcgctttcac gaacgtcagc gcgtccgcgc gcttcgcgag ctgcatcgcg
     5341 atatcggtga cgatcatcgc aacgcgcgcg ccaccgacag aattgatctt gtcggcatcg
     5401 tcggtcgcgg tgtgatagtc gaggtggttg ccggtgaaga agaagagcac cggaatgccc
     5461 gcgacgtaga acgccatgtg atcggacggg ccgtagcccg agccaccgac ggtgcacacg
     5521 acgcgcgctg catcgcacgc tggatcgacg agcgtgtgcc actccttcgc ggactcggcg
     5581 ccgttgacgt ggagctggtt gttccgcatg cggccgacca tgtccatgtt caacatcgcg
     5641 acgacgggct ccttcgtcgg cggattgcga acgaactgcg ccgaaccgag gtcgccctct
     5701 tcctcgcccg agaacgcgac gacgtagaca tcgcgctgca gctccgctct cttcgtgccg
     5761 agcgcggcgg ccacttcgag gagcgcagcg acgcccgatg cgttgtcgtc ggcgccgttg
     5821 tgcacggcgt gcactttggg atcgagcgcg ttgctgccac cacccatgcc gaggtgatcg
     5881 acatgcgcgc cgatcacgat cacgccgggc agcttgttcg cagcgccggc cttgatcaat
     5941 ccgacgacgt tatcggtcgg cgtgcgcacg ggctcgagtg cgaccgtcag cttcaccttg
     6001 tggttgccct ttgcgaggcc cgcagctgca tcgcgcttga gcacgatgat cggcagtccg
     6061 gcgtccgcac cgccggagac gagatcggcc atgccgtcgg tcgcgagcga aggcagcggc
     6121 gcttcatcct gcttcgggtc gccatcgtcg acaacgatga ggccgacggc gcctttgccg
     6181 cgcgcggtga acgccttgta gcgaatgtcg ccgaggcgcg cggagctcgt cggaccgagg
     6241 ccgtcgacgc tcggcacgaa gcggtgaacg agcacgaact tgcccttcac gttcttgcct
     6301 ttgtagtcgt ctagcttcgt ctctgcgtcg acgatgcccc accctgcttc gacgaccgcc
     6361 gcgctgacgg tcttgctcgc cgagaacggc atcggcgcga acatctcggg cgccacggct
     6421 ttgccgtcga gctcgagtgc cgtcgacgcg ccgcgcttca cttccgtcgt cacatcgaac
     6481 gtctgccgcc acgcgccgtt gtagccaggc tcggctccag ccgcggcgag ttgctgctgc
     6541 acgaagtctg ctgcatcacg caggcccttc gttccgatgc cgcggccttc cgtcgcatcg
     6601 tcggcgagtc tgtagatcgc atcgcgatag cgatcggcgc tcaccgtctc gaccgcgccc
     6661 gtcggcggcg gcgaattcac ccactcagcg acgaacacgt tcgtgtcgtg ctcgccggcg
     6721 ggcgcaccgg tcgcgcgata cttcccgcca acgacatcct gccggttcga cgagaacgcg
     6781 agcttcgtgc cgtcgggcga gaacaccggg aagccatcga agcccggcgc gttcgtgatc
     6841 tgctcgaggt gcgtgccatc gatgtcgatc gcatagatgt cgaactcggg accgcgcgga
     6901 ttcttgaagt tcgacgaaaa caggatccgc ttgccgtccg gaaagaagaa cggcgcgaac
     6961 gatgcgccgg gcaggtacgt cacctgatgc gcttcggttc cgtcgccgtt tgcgacccag
     7021 atgtccatct tcgtcggctt gacgaggttc tgcgcgagca gctgcttgta cgcgtcgagg
     7081 tcgttgccct gcggccgcga cgagcgccac acgagcttgc tgcaatccgc ggagaagaac
     7141 gcgccaccgt cgtagcccgg caggttcgtc agctgtcgca agtttccgcc gtcggcgtcc
     7201 atgcgccaca gctcgagatc gcccgagcgc atcgacgtga atacgatcga cccgtctttt
     7261 ccgcacacgg tcgcttcggc gtcgtagccc ggcgccggtg cgagcttgcg cagattcgat
     7321 ccgtcgggcg cagccttgta gatgtcgtag tcgaacaatc cccacagata gcccttcgac
     7381 atatccggcg gggtcgggca ctcggccgac acttcatgcg tcgacgcgta gatgatctct
     7441 tggtcgcctt tgaggaaata cgagcacgtc gtgcggccct tgcccgtcga cacgagcttc
     7501 gcgggaccgc ccgtcgaggg catcacttcg atctgatcgc atttatgcgg ctgatggttc
     7561 gtctggaaga tcagccgatc gccggagaac gaccagtacg cttctgcgtt gtcgccgccg
     7621 aacgtcaact gccgcacgtt cttcaggtgc gtctcaccgg gcgcggcaag ctccttcgtg
     7681 tcgggcgcag gcggagtcac cgtcgtgatt tttggtttcg cagcctcggg cggacagcac
     7741 gccgccacaa tcacgagcag caagtgacgc ttcatcgcgg cgtagagtac cttccccgtc
     7801 cccatgctgt cagtcgagga agcggccaag accgtggcgt cgcggatcgc gcgcctgccc
     7861 gtcgagaacg tgtcgctgca cgacgcacgc aaccgcatcc tcgccgccga cgtcgtcgca
     7921 acgcgcaact tgccgggctt cgataactcc gccatggacg gcttcgctgc gcggtccgcc
     7981 gagcttccag caactctgcc ggtcgtcgcg aaagtcgccg ctggccaagt gctcaccgac
     8041 ctgattccag agcgcgtcgc gatccggatc atgacgggcg cgccgatgcc aaagggcctc
     8101 gataccgtcg tcatccaaga agatgcacgc atcgacggca gcaacgtcac gctcccggca
     8161 gcacctgccg gcgacaacgt tcgcaaagcc ggtgaagaca tcgcgatcgg cgaaatcgcg
     8221 gtccgcgccg gggaaagact cgacgctgca gagctcggct tactcgccgc gctcggggtc
     8281 gtcgaggttc ccgtctcgcg ctcgccgaga gtcgcaatca tcgcaaccgg cgatgagctc
     8341 gtcgcagcgg gaagcgcgct cggtccagga cagatcgtcg actcgagcgc gcacacgctc
     8401 gccgctctca ttccgacatg ctgtggcgtc gtgagctaca tcggcatcgc gaaggatgat
     8461 ccggcggcga tgaccgcgtt gatcgcatcc gcaatggatc acgacgtcgt gatcacgacg
     8521 ggcggcgtct cggtcggcga tcgcgatcac gtgcgtggag cgctcaaagc ggcgggcgtc
     8581 gagctcgagc tgtggaaggt cgccatgaag ccgggcaagc cgttctcgtt cggggtgagc
     8641 ggccgcgtcc cggtgttcgg tctgcccggc aacccggtct cgtcctacgt cgcgttcgag
     8701 ctgttcgtgc gccctgctct cctcgcgatg caaggagcca cgctgaccaa gcgccctcgt
     8761 gctccggtgt cgctcgcgcg cggctatcgc aagcaagcag gtcgcacgca ttacctccgc
     8821 gcaaagctcg ttcgcaacgg cgagcagctc gtcgcccatc cgcatcccaa gcaaggctcg
     8881 gcgatgctca cgtcgctcgt cggctgcaac ggtctcgtcg agctgccggc cgatgcgacc
     8941 gaagtcttgc ccaacacgat cgtccccgcg atcctcctcg aggctgtatg agctcggtca
     9001 acatcaccgt tcgcgtcgaa gacgaaggcc gcatcgacaa ggcgctcgcg aatcattatc
     9061 cgaatgccgg acgacgccag ctctccgagc tgttcgacga aggccaggtc aagctgcgcg
     9121 gcaagcgcgc gaagaaaggc gatcgcgttg ccgccggcga cgtcatcgag ctgttgcgcg
     9181 ccccggtgag cggcacagat ctgcagcccg ttgccgatcc cgaggtcgag ctcgatctct
     9241 tgctcgagcg gcccgagctc gtcgtcgtga acaagcctgc cggcattcca tcgcagccgc
     9301 tgcgtgcagg cgagcgcgcg acgatcgcga acggcatcgc ggctcgcttc cccgagtgca
     9361 tcaccattgg tgacgatcct cgcgacggtg gcctcgtcca tcggctcgac atcggcacgt
     9421 cgggagcgct cgtcgtcgcg cgaaccgccg aggcgtatcg aacgctgcgc gacgcattcg
     9481 gccaaggcct cgtcgagaag cactacctcg cgatcaccga taaccgaccc gtatcccgcg
     9541 agtgcgacgc gccactcgtc cagcgcggcg atcacgtgcg cgtcgatcac accgacggtc
     9601 tcgcggcgta cacgtcgttc accgtagaga aagcatcgga gtcgcacgca ctcgttcgct
     9661 gcgtcgcaca gaccggccgc atgcatcagg tgcgcgcgca tctcgcacac gtcggctcgc
     9721 cgatcgcggg cgacactctc tataaaggca agccgctcgg cgacgacgcc ggcttctatc
     9781 tccacgccgc gctgatcgtg tttcccttcg gcgcagaaaa gatccgcgtc gaagcaccgc
     9841 tgcccgaacg cttcaccgcc gcgctcgccg ctgcgggtct cgggtagcgc ttcggttagc
     9901 gatcaatccg tcgcttcagt gcggaactcg accgtctcga gcccgaggcg cttgagcgcg
     9961 tcgtagatct ctttgcgcgt cttctgtccg atcttcgacg cgagcgacac gttcccctcg
    10021 gcgacgcgca tcaggcgcga gtagtactcc ttctcgaatg cctgctttgc gtctttgtac
    10081 ggaaggatcg aggcggtcgt cgggccactc gtcgccgacg tcggcagcat gatgtgatcg
    10141 ccttcgatca cgtcttcgcc cgccatcacc gcggcgcgct cgagcgtgtt gacgagctcg
    10201 cgaacgttgc cgggccagga atgcgcgagc atgcgctcga gcgcgagcga tgacagccgc
    10261 agcgtcggct tcttgtcgcg ctctgcaatc cgcgagaggt agtgattcgc gatcggcggg
    10321 atgtcttctg gacgttcgcg aagtggcggc acgcggatcg gatagacgtg gatgcggtag
    10381 tagaggtctt cgcgaaatcg tccgagcttc gtctccgcag cgagatctcg attggtcgca
    10441 gtcacgagtc ggaagttgat gaagcgcggt tccgtcgaac cgaggcgcgt gacctcgttc
    10501 gtctgcagca cgcgcagtag ccgcacctgc atcgccatcg gcatctcgcc gatctcatcg
    10561 agaaacagcg tgccgttctc cgacgactcg atcgcgccaa catgcgtagc gaccgcgccc
    10621 gtgaacgcgc ctctctgata gccgaacagc tccgcttcga gcagcgtctc gggaatcgcg
    10681 ccgcagttga gcgcgacgaa cggcgcagcg gcacgcggcg agctgaagtg cagcgcgcga
    10741 gcaacgagct ctttgccgac gccgctctcg ccttggatga gcaccgcggt ctgcgcgggc
    10801 gcgagctgat cgagcgtgtg cagcaagtcg cgcacgtgct ggctcgtgcc gagaatcatg
    10861 tcccccttgc gccgccacgg agatttcgtc tccgcgccga tcatggtcgt gaggatcgcg
    10921 ccgagcagtg gtgctggcgg ttcggcaggc agccagatgt ccggcttcgc ggcgtgcacc
    10981 gggttcttgc cgatcacgag cagcggcatt ccttccggcc gtgcacgcaa catgttcgcg
    11041 agcgtgtcgg cgacctcggt gttcgagccg agcaggtaca tcacgatcgg ggtcggcggc
    11101 aggtcatcgg tcccgtcggc ctcgatcacg gcccacgatc cggccttcgc gagcagccgc
    11161 cggatacccg atcgatcacc cggccggcca aaaacgcaga cgcttattga cgttatctcg
    11221 ctcacgggaa tcgaggcgcg tggatgagaa acgatacacc caatctcgag gctcccaaat
    11281 tttctcgtga tctcgtcgac ttgaatatcg ttcgcacgat cgcgctagga ccacgtttaa
    11341 attcaatagt ttaagcggga gtgtatgagt ttccatccac gggcggatct cacgaaggta
    11401 agaaaccgaa tggttccgcg cgcctaagac acacggtgcg ttggcgctcg acctgcagat
    11461 agtccgtctc actatgggga agacgtatgt ctggatgttg gccctcggag tcgccggctg
    11521 tacgacggat cccgaagtca cctccaccga agagttcctg aagaggaaca acggcttcat
    11581 cccgaacggc gtccaggtcc cgaacgacca cgggtcgtcg tcgacctacg cccgcagcgg
    11641 caagatcgac ctcaacaacg agttcttcca ggacctgggt acgaacggtc gccgctgcat
    11701 cacgtgccac ctcccgaccg cgggctgggg catcacgccg gagcaagtcc aggcgacgtt
    11761 cgagctgacc cgtggcggcg tcaagaacga cggcttcggt ctcggcgcga tcttccgcac
    11821 caacgacggc gcgaacagcc ccgactaccg cgctgaccag ctcgacacgg tcgccgagcg
    11881 ccgcgctgcg tactcgatgc tcctcaacca tggcct
//
LOCUS       DATLBZ010000160         6337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4618519, whole genome shotgun sequence.
ACCESSION   DATLBZ010000160 DATLBZ010000000
VERSION     DATLBZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4618519"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..394
                     /locus_tag="VMZ53_08465"
     CDS             158..394
                     /locus_tag="VMZ53_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28528.1"
                     /translation="MQWLEHDLARQGLQDLDDAWKRLGSEDGWPKSICTNMATPEAPH
                     GTATCGAIAMNMDTGELWAQQGLIHNVAPEKWQL"
     gene            468..1793
                     /locus_tag="VMZ53_08470"
     CDS             468..1793
                     /locus_tag="VMZ53_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUS28529.1"
                     /translation="MSEYPFFFTWTAQNAAKPVELAGGEGAWFTLKDGSRWLDLGALA
                     YQVNVGHGNKRIVEAIKRQADELCLSTPNAVFPAKVELAQRLLKMAGPAFSKVFFTLG
                     GAEANENALKMARLATGRLKNIARYRSYHGASMGALSLTGDWRRAPVEPGIPGVVHVL
                     DCYCDRCPFGKTVDTCMRECATNIGDVMRLESAGAVADLSARGLAGQGRGPAGAVAAV
                     FLEPVPGANGVLVPPPEYWPIVRKACDDEGALLVADEVLTGFGRTGKAFGHQHWGVTP
                     DLITVAKGLSSGYATIGAVIVSDKIARYFDENVLAAGLTYYAHPLACAAAVETLKVYE
                     DERMFEHAAALGPVLRAELEAIKGRIAAPAFVRSLGLLGALEIEAPLPSWQTLGRELA
                     SRKLSLHVDGKRGTAIFSPPLCITEQELVTGMRSFGDAAVAAFGARAAL"
     gene            1790..2506
                     /locus_tag="VMZ53_08475"
     CDS             1790..2506
                     /locus_tag="VMZ53_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HUS28530.1"
                     /translation="MSGRHSNKVCESVAKALEPLRDGMTIMCGGFGLSGNPEALIRGV
                     VERGVRDLTLVSNNAGNMGKGLATWLKAGIIKKVICSYIGNNDDLHTRMASGEVQVEI
                     TPQGTLAERMRAAGAGIPAFFTPTGVGTVVAEGKETREIDGRTYVLERSLPADVAIIR
                     AAKSDRFGNVRFWRTARNFNPVMATAAKLTIAECDELVENGAIDPDDVHLAGIFVHRI
                     VHVPEHENAFEYKTLRKRPS"
     gene            2503..3147
                     /locus_tag="VMZ53_08480"
     CDS             2503..3147
                     /locus_tag="VMZ53_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HUS28531.1"
                     /translation="MSSTTPGWSTEEMARRAAREIPAGCIVNLGIGLPTQVADYLPEG
                     HAWLHSENGLLGTGPFPYEGDEDPNLINAGKQTVTVLPGGSTFDSASSFGMIRGGHVD
                     LAIMGAMQVSAGGDLANWAVPGGKVMGIGGAMDLAAGARRLIVMMQHVTKKKEFKLVG
                     KCDYPLTATRVVSLVITELGAFEPTGHGFRLIELAPGISREQAESSTGAPLIDA"
     gene            complement(3230..3781)
                     /locus_tag="VMZ53_08485"
     CDS             complement(3230..3781)
                     /locus_tag="VMZ53_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28532.1"
                     /translation="MRAFLVIAFCLAAAGCKKESSDVSISDKGVSVKQGDKTVDVTAS
                     GVATKSGSDKVDVTANGVVAKSGDTAVDVGVDVAHAKAMAEGAVKVANEVTPATGAMQ
                     CGMGGCTQTCEMGRTCDFSCTGGGCKQHCPQSSICTFSCTGGNCKQTSEDGAKVTMNC
                     TGGNCTQKCDGPCEKSCTGDDCK"
     gene            complement(3822..4253)
                     /locus_tag="VMZ53_08490"
     CDS             complement(3822..4253)
                     /locus_tag="VMZ53_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28533.1"
                     /translation="MRGILLVAALCAGCWSSSGGAKDPAPLPDPKPSDPPPDPAIAML
                     TSVVVVEACPEAKTIDTKRATREIDELVGPCKQVPGGSAHFAATLLPDGRVELASPTG
                     DPTMGVVPTCLMQKANQLKHKLRLKQACKFDVKLEQRGEQR"
     gene            4315..5355
                     /locus_tag="VMZ53_08495"
     CDS             4315..5355
                     /locus_tag="VMZ53_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28534.1"
                     /translation="MAAGLDSFDDVRTLADEELRALLESGRPEQRVWAMWALALRSAH
                     VNDIVDLGTRREPDAGVRRNLAVVLAGHGQIDLLVALAKRDPAPEVRAAAMQLVSRLA
                     IDGKVPALLVTERVASDATEVRIAVLGTIFENAPPWLIEVAERLLEDRDNEVRYEAFE
                     AMLRVGRELQALMWLEEAPEAEARLALMRWSSRGRTRECALALAKASRRLRRLLVESV
                     RLASWRDLEPAIGEDITLVRAVARRNPNVFDEMPLAALMRATLREPTPAWIALIRDRF
                     SLLDHPPDEVASLLHDFRELCTKQITECDHALARMRGQRDDDLERGVDYLQEQRGVLE
                     AAYDHAARMLVH"
     gene            complement(5352..>6337)
                     /locus_tag="VMZ53_08500"
     CDS             complement(5352..>6337)
                     /locus_tag="VMZ53_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUS28535.1"
                     /translation="PGGDQYGDHLIWRTAAATQAGAAPILLVGHHDTVFPPGHFEGWR
                     EEGDRAIGPGALDMKGGLAIIRATLAALDEVGALAKLPIIVVCVADEEVGSPTSAAHL
                     KELAKGASCALVFESGRANDMIITRRKGVGAMTVIAHGKAAHSGNNHKDGANAIWALS
                     KFVDAAQKLTNYERGVTVNVGQFTGGTSKNTVPERAECKIDLRYETVADAEALVADLK
                     AAAAAASVPGVTLEVIGGVNRLPLERTDASAALRDEYAACARASGLGDGEAPLLGGGS
                     DANTVAPLGVPAIDGLGPRGAGFHTSTEYVELHTFVPKAEALVRFLVGRLR"
BASE COUNT         1006 a         2180 c         2087 g         1064 t
ORIGIN      
        1 atcggctcgg gccaccacta cttcgtcgcc gacaagacct ccgccttctc gctcgaagcc
       61 agcggcacgc gccgccgcct cgtctttgcc ggagagcggt cgcagttctg ccacacgaac
      121 cactgcctcg acgccgacgt cgacgacgca cgagcgcatg cagtggctcg agcacgacct
      181 cgcgcggcaa ggcctgcaag acctcgacga cgcgtggaag cgcctcggct ccgaagacgg
      241 ctggccgaaa tcgatctgca cgaacatggc gacccccgaa gccccacacg gcaccgccac
      301 ctgcggcgcg atcgcgatga acatggacac gggcgagctc tgggcccagc aaggcctgat
      361 ccacaacgtc gccccggaga agtggcagct gtgaccgcgt taattcagtc cgacctaatt
      421 tctggtcaga caacaaattc agtccgacct aatttcctgc ggcagcgatg agcgaatacc
      481 ccttcttctt cacgtggacg gcgcagaacg cggcgaagcc cgtcgagctc gcgggcggcg
      541 agggggcgtg gttcacgctg aaagacggca gccggtggct cgacctcggt gcgctcgcgt
      601 atcaggtcaa cgtcggccac gggaacaagc gcatcgtcga ggcgatcaag cgacaagccg
      661 acgagctgtg tttgtcgacg ccgaatgcgg tgtttccggc gaaggtcgag ctcgcgcagc
      721 gcctgctgaa gatggccggg cccgcgttca gcaaagtctt cttcacgctc ggcggcgccg
      781 aggcgaacga gaacgcgctc aagatggcgc gcctcgcgac ggggcggctc aagaacatcg
      841 cgcgctatcg cagctatcac ggcgcgtcga tgggtgcgct ctcgctcacc ggcgattggc
      901 ggcgcgcgcc cgtcgagcca ggaattcccg gcgtcgtgca cgtgctcgac tgctactgcg
      961 atcgctgtcc gttcggcaag accgtcgaca cgtgcatgcg cgagtgcgcg accaacatcg
     1021 gcgatgtgat gcggctcgag agcgcgggcg ccgtcgcgga tctgagcgcg cgcggtttgg
     1081 cggggcaggg acgcgggccg gctggcgcgg tggcggccgt gtttctcgag ccggtgccgg
     1141 gcgcgaatgg cgtgctcgtc ccgccgccgg agtactggcc gatcgtccgc aaagcctgcg
     1201 acgacgaggg cgcgctgctc gtcgccgacg aagtgctcac cggctttgga cgcaccggca
     1261 aggcctttgg ccatcagcac tggggcgtga cgcccgactt gattaccgtc gcgaagggat
     1321 tgtcgtcggg ctacgcgacg atcggcgccg tgatcgtcag cgacaagatc gcgcgctact
     1381 tcgacgaaaa cgtcttggcc gcaggtctca cgtactacgc gcatccgctc gcgtgtgcgg
     1441 ccgccgtcga gacgctgaag gtctacgaag acgagcgcat gttcgagcac gcggcggcgc
     1501 tcggccccgt tctgcgcgcg gagctcgaag cgatcaaagg gcgcattgcc gcaccggcgt
     1561 tcgtccgttc gctcggcttg ctcggtgcgc tggagatcga ggcgccattg ccgagctggc
     1621 agacgctcgg tcgcgagctg gcgtcgcgca agctgtcgct gcacgtcgac ggcaagcgcg
     1681 gcaccgcgat cttctcgccg ccgctctgca tcaccgagca agagctcgtg accggcatgc
     1741 gttcctttgg cgacgcggcc gtggcagcat tcggtgcccg agcagcgtta tgagcggtcg
     1801 ccactcgaac aaagtttgcg agtccgtcgc gaaggcgctc gagcctctgc gcgacggcat
     1861 gacgatcatg tgcggcggct tcggcctctc gggcaatccc gaggcgctga ttcgcggcgt
     1921 cgtcgagcgc ggcgttcgcg acctgacgct cgtctcgaac aacgcgggca acatgggcaa
     1981 gggcctcgcg acgtggctca aggccggcat catcaagaaa gtcatctgca gctacatcgg
     2041 caacaacgat gatctgcata cgcggatggc gtcaggcgaa gtgcaggtcg agatcacgcc
     2101 gcagggcacg ctcgccgaac gcatgcgcgc ggcaggggcg ggcatccccg cgttcttcac
     2161 gccgacgggc gtcggcacgg tcgtcgctga aggcaaagag acgcgcgaga tcgacggccg
     2221 cacgtacgtg ctcgagcgtt ccctccccgc cgatgtcgcg atcattcgcg ccgcgaagag
     2281 cgaccggttc ggcaacgttc gcttctggcg caccgcgcgc aacttcaatc ccgtcatggc
     2341 gaccgccgca aagctcacga tcgcggagtg cgacgagctc gtcgagaacg gcgcaatcga
     2401 tcccgatgac gttcacctcg cgggcatctt cgtgcaccgc atcgtccacg tccccgagca
     2461 cgagaacgca ttcgagtaca agacgctcag gaagcgcccg tcatgagcag cacgacgcca
     2521 ggctggtcga ccgaagagat ggctcgccgc gccgcgcgcg agattcccgc cggctgcatc
     2581 gtcaacctcg gcatcggcct gccgacgcaa gtcgccgact acctgcccga aggccacgcg
     2641 tggctgcact cggagaacgg cctcctcggc accggtccgt tcccctacga aggtgacgaa
     2701 gatccaaacc tgatcaacgc cggcaagcag acggtcaccg tgctgccggg tggctcgacg
     2761 ttcgattccg cgtcgtcctt cggcatgatc cgcggcggtc acgtcgacct cgcgatcatg
     2821 ggcgcgatgc aagtctcggc cggcggcgac ctcgcgaatt gggcggtgcc gggcggcaaa
     2881 gtcatgggca ttggcggcgc gatggatctc gctgccggcg cgcggcggct catcgtgatg
     2941 atgcagcacg taacgaagaa gaaagagttc aagctcgtcg gcaagtgcga ctacccgctc
     3001 accgccacgc gcgtcgtctc gctcgtgatc acggagctcg gcgcgttcga gccaaccggc
     3061 cacggcttcc gcctcatcga gctggcgccg ggcatctcgc gcgagcaagc cgaatcgtcg
     3121 acgggcgcgc cgctgatcga cgcgtagctg ctggctcgct cgtcgtcgct cgccgcggct
     3181 cgtcgtcgct cgtcgtcgct cgtcgtcgct cgtcgctgtt ccgaggccgc tacttgcagt
     3241 cgtcgccggt gcagctcttc tcgcacgggc cgtcgcactt ctgcgtgcag ttgccgccgg
     3301 tgcaattcat cgtcaccttc gcgccgtctt ccgacgtctg cttgcagttg ccgcccgtgc
     3361 agctgaacgt gcagatcgag ctctgcgggc agtgctgctt gcagccgccg ccggtgcagc
     3421 tgaaatcgca ggtccggccc atctcacacg tctgggtgca gccacccatg ccgcactgca
     3481 tggcgcccgt cgccggagtg acttcgttcg cgaccttcac ggcgccttcg gccatcgcct
     3541 ttgcgtgcgc gacatcgaca ccgacgtcga ccgccgtgtc gccgctcttc gcgacaacgc
     3601 cgttcgccgt gacgtcgacc ttgtcgctgc cgctcttcgt cgcgacgccg ctcgccgtga
     3661 cgtcgaccgt cttgtcgcct tgcttgacgc tgacgccctt gtccgagatc gagacgtccg
     3721 acgactcctt cttgcagccg gcggccgcaa gacaaaacgc aatcaccagg aatgccctca
     3781 tccctcgatc gaacgcggac gggccgcgga acgcaagcgc ctcagcgctg ttcgccgcgt
     3841 tgctcgagct tgacgtcgaa tttgcacgcc tgtttcaagc ggagcttgtg cttgagctgg
     3901 ttcgccttct gcatcaggca cgtcgggacc acgcccatcg tgggatcgcc cgtcggcgag
     3961 gcgagctcga cgcgaccatc gggcaacagc gttgcggcga agtgggcgga gccgccggga
     4021 acctgcttgc acgggccgac gagctcgtcg atctcgcgcg tggcgcgctt cgtgtcgatc
     4081 gtcttcgctt cggggcaggc ctcgaccacg acgaccgacg tgagcatcgc gatggcgggg
     4141 tcgggcggcg gatccgaagg tttcggatct gggagtgggg cagggtcctt cgcgccgccg
     4201 gagctactcc agcaacccgc gcaaagtgcc gccaccaaga ggattccccg cacgtcggga
     4261 tcatgtcatc aggtctgacg agcgcgccaa catttcgccg ctacactggt agcaatggct
     4321 gccgggctgg acagcttcga cgacgtacgc acgctcgccg atgaggagct gcgcgctctc
     4381 ctcgagtcgg ggcgccctga acagcgcgtt tgggccatgt gggcactcgc gttacggtcc
     4441 gctcacgtca acgacatcgt ggacctcggc acgcgccgtg agcccgatgc cggggtccgc
     4501 cgcaacctcg cggtcgtcct cgcaggccac ggtcagatcg acctgctggt cgctctcgcg
     4561 aagcgcgatc cggcgcccga agtgcgcgcg gcggcgatgc agctcgtgtc gcgtctcgcg
     4621 atcgacggca aggttcccgc gctgctcgtc accgagcgcg tcgcgtcgga cgccacggaa
     4681 gttcgcatcg ccgtcctcgg tacgatcttc gagaacgcgc cgccgtggct tatcgaagtc
     4741 gccgaacgcc tcctcgaaga tcgcgataac gaagtccgct acgaagcgtt cgaggcgatg
     4801 ctgcgcgttg gccgcgagct ccaggctctc atgtggctcg aggaggctcc ggaagcggaa
     4861 gcgcgcctcg cgctgatgcg ctggtcctca cgcggccgca cgcgtgaatg tgcgcttgca
     4921 ctcgcgaagg cgtcgcgtcg cttgcgtcgt ctgctcgtcg agtccgtgcg cctcgcgagc
     4981 tggcgcgatc tcgagcctgc gatcggcgaa gacatcacgc tcgtgcgcgc ggtagcccgc
     5041 cgcaatccaa acgtgttcga cgaaatgccg ctcgccgcgc tcatgcgcgc gacgctccgc
     5101 gagccgacgc ctgcgtggat cgcgctgatt cgcgaccgct tctcgctgct cgatcatccg
     5161 cccgacgaag tcgcctcgtt gctgcacgac ttccgcgagc tgtgcacgaa gcagatcacc
     5221 gagtgcgatc atgcgctcgc gcgtatgcgt ggccagcgcg acgacgatct agagcgcggc
     5281 gtcgattatc tgcaggagca gcgcggcgtg ctcgaagcgg cgtacgacca cgcggctcgc
     5341 atgctcgtgc actagcgcag gcgaccgacg aggaatcgca cgagcgcttc ggccttcggc
     5401 acgaacgtgt ggagctcgac gtactccgtc gacgtgtgaa agcccgcgcc acgcggaccg
     5461 aggccatcga tcgcaggtac gccgagcggt gcgacggtat tcgcgtccga gccgccaccg
     5521 agcagcggcg cttcgccgtc gccgagcccg gatgcacgcg cgcatgccgc gtactcgtca
     5581 cgcagcgcag ccgatgcatc tgtgcgctcg agcgggaggc gattcacgcc gccgatcact
     5641 tcgagcgtca cgcccgggac cgacgctgcg gcggccgctg ccttcagatc tgcgacgagg
     5701 gcttccgcat ccgcgacggt ctcgtagcgc aagtcgatct tgcactccgc gcgctcgggc
     5761 accgtgttct tcgacgtgcc ccccgtaaac tggccgacgt tgacggtcac gccgcgctcg
     5821 tagttcgtca gcttctgcgc cgcgtcgacg aacttcgaca gcgcccagat cgcgttcgcg
     5881 ccatccttgt ggttgttgcc gctgtgcgcc gctttgccgt gcgcgatcac cgtcatcgct
     5941 ccgacgccct tgcgccgcgt gatgatcatg tcgttcgcgc ggccactctc gaacacgagc
     6001 gcgcacgacg cgcccttcgc gagctccttg aggtgcgccg cggacgtcgg cgatccgact
     6061 tcttcgtcgg cgacgcagac tacgatgatt ggcagcttcg cgagcgcgcc gacttcgtcg
     6121 agagcagcga gcgtcgcgcg gatgatcgcg aggccgcctt tcatgtcgag cgcgccggga
     6181 ccgatcgcgc gatcgccttc ttcacgccag ccttcgaagt ggccgggcgg gaacacggtg
     6241 tcgtgatggc cgacgagcag gatcggcgcc gcgccggcct gggtcgctgc cgcagtgcgc
     6301 cagatcaagt gatcgccgta ctggtcgccg ccgggaa
//
LOCUS       DATLBZ010000161         7389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_897682, whole genome shotgun sequence.
ACCESSION   DATLBZ010000161 DATLBZ010000000
VERSION     DATLBZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897682"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VMZ53_08505"
     CDS             <1..294
                     /locus_tag="VMZ53_08505"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829720.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HUS28536.1"
                     /translation="SEKALKKAGMTPKDVDLWEINEAFAVVPLQTARKLNIDLDRVNV
                     NGGAIALGHPLGGTGAILLGTALDELERANKSTALITLCIGGGMGIATVIERV"
     gene            342..2933
                     /locus_tag="VMZ53_08510"
     CDS             342..2933
                     /locus_tag="VMZ53_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUS28537.1"
                     /translation="MRRIAVLFALAACSEPAPKQAPRRNWVYPPAPAAWPLGSVTATI
                     GRSQAPDIALIEGIEAGPNAGPLTPLRMPTPWPVPDGGAAGAHARAVIYGTLGAQHVV
                     ELIDIDAGRLLWRDTSACRAPVVAVTAEAVVCADAKGVRAIAIADGKQRWKSDATFIA
                     MTDDRVITAGAGESVIVDANDGGELARVKLPPSVQSDSIIASCGDAGRELFAYGQDGR
                     LVHVIEAKGGPTIQWSLAVESFTTIDACEGPSVIVASGTSLLAVARATGVVSGSIDGV
                     RGYWPARDGSDKLEVSTSTGVALRARDLRGPSESVGLPPLGELLSKRGDMRLVRATPS
                     TAVVLDSKGVRAYLPLAQLGAVLGDNAIIAGSWTGSPGETVRRIAIPDGRYRKRLRLP
                     RVGRGLGVTAELRDLPAAQDIDASGAIAKNDAGKAAVAAVALDPSGAAVYATALDGEP
                     DENSSAGVARFDLGSRTWTWYRADGCGSGAPIALAASNEVIVCGARAGSTTAPARVGG
                     GTAAKGDGGRASVRATTRDGKSAWTWHSDNVDGLDAIRALVLVRDAARVHVLDAAGGH
                     LLGDFESDDGNAMPAAIVSVDFDATSASRGSDATSASRGSDSVSAKHGDSMEMLVVAE
                     HGRVVGRLPRAQMVPAWSIAVNGVVKLIVAAGDGVLVELEDGDAYRIDARTGATMPVA
                     GIGLDWHASDDLLAGDTPGGPVPPQDMLDRVPTSDEPPAAPAPAKKSRKKAGKKDDKP
                     KEVVDESLGIPPRLPPVWPDPGPLPASWQLTIYEPNGAFRARNDYALEAPITPATARS
                     AKAPIVVQSGPELRDILLLDPGTGDPLRRVRLPEDAAPGTAFSTIVDGKAVVGVLLAN
                     PLRVVVF"
     gene            2961..3620
                     /locus_tag="VMZ53_08515"
     CDS             2961..3620
                     /locus_tag="VMZ53_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28538.1"
                     /translation="MALPLRAALRSAGRFIAKNPTTMLSMARHALGMRLAVPLDAVRW
                     FIANTPPSKKAPQDVTITPRPPAIGLGATVDLMGTTVRAGASVRIDQIEVGDEKLRVT
                     LRISDVTMKVLGDSATPVAGLIKSGALDLSKPGNLAKFMPKKSPVLVEAHDDIVVLDL
                     MQNPKIAANDRVRGVLSTLTPVVQVSGLSTEGDFLIIQLRASPFGLPRAINAARQLAA
                     G"
     gene            3629..4117
                     /locus_tag="VMZ53_08520"
     CDS             3629..4117
                     /locus_tag="VMZ53_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUS28539.1"
                     /translation="MAESVAFDASADKPARYRELLPQLRSLVDGEPDLIANLANITAA
                     LRECLPVASWIGWYVKRGDELVLGPFQGKVACVRIAFGKGVCGTAAAEKRTVIVPDVE
                     KFPGHIACDAGSRSEIVLPIVRRGEVVAVLDLDSYELAAFDDVDAEWLAQVAELASTF
                     AW"
     gene            4114..5001
                     /locus_tag="VMZ53_08525"
     CDS             4114..5001
                     /locus_tag="VMZ53_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28540.1"
                     /translation="MSRLALIAALLAAGCQDSGTPKQTARKDAPLTASTDDADPTDAP
                     PPADEPTEPAPDPGKLVADLGAIPAWQAVVDRGEYLQRRGQHGIVYGTLGPTIQIADP
                     ASDGGLINSPYTWLVDDTEGNGCLAIRVALGNKTPKLGDRVALAGAWKLDDKLEYFWD
                     VAELTQLPPGPPSDLKDPPSPPGHALQEGPLPPGTRMISLAKDNDLVYFQVTGRVPLV
                     DGDGWPVADQLGNTVVALLNLPGERPSFGAQDFRTKNERWQLKRGITYFVRIGKVRKN
                     KDPLKPANINARTAPVRMK"
     gene            complement(5002..5697)
                     /locus_tag="VMZ53_08530"
     CDS             complement(5002..5697)
                     /locus_tag="VMZ53_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28541.1"
                     /translation="MWAGASSCVAALLAAGCYHSTPPPRMTPIEQPVKPDAPVVVEQP
                     DTSLYTYEEVFADIASGPLVYVGTGEWTGMFHVYACAYRNAKVYVINFYCTKSYEKKA
                     FGIAVLSPTRGRAYLYAEADKPISGLRRADYFTYRFEAELNTTPPVSMTFSYDQLHAW
                     DEARYKQYASGCFAGVELRKPVNGCMQKLEEFETSFPDSHRAILENPPEAYYALVRDL
                     RDRAKREGRDWKK"
     gene            complement(5678..6625)
                     /locus_tag="VMZ53_08535"
     CDS             complement(5678..6625)
                     /locus_tag="VMZ53_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28542.1"
                     /translation="MKKAIVVLMLLSATAFAETPWEQGVPKDKRDQAEALFREGNTFF
                     AQQAHQPALEKYNAAIQLWDHPLIRFNMAVTLIRLDKILEAAEDLDSALRFGQQPFTP
                     ELYQQALDYQKLVSGRVGTVEATCDQSEAQILLDGKPWFSCPGSKKQRVLSGEHAIVG
                     EKKAYMTVSRRVVVAGGGTASEKIALLPIESVVKLEYSTPRWIPWTVAGGGAAIGLGG
                     LGFWFAGRNQMDQFEADFAMRCPTGCNADLSDQPDLARDRDSAELKGKIAVSMMVAGG
                     GLAIGGVVWAIMNRPKRVLPDMEVQPTNGGVAARVGWRF"
     gene            complement(6622..>7389)
                     /locus_tag="VMZ53_08540"
     CDS             complement(6622..>7389)
                     /locus_tag="VMZ53_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28543.1"
                     /translation="PTLKRWLEDSQAMRGFMHELRQAAKQWASRGKPADLVWRGATAQ
                     EALGHVKRHVLDLSTVEREFLAEVKAQTARSRRRKLAVFTAIAVTLALVLAGGSFALV
                     RIKLAEKDAQDKAAQAIKAKKEAEGALGQAKQATEEAVKAKGDLQGKLDIIEAEKKQR
                     LAAEKLAEDATKSTEMTKEQLAEANKELQRKVAEAQAAKEKALASEAAAKKATEDAKV
                     AKTQVERMLEKQKAENERLRAESSKIFNGSLSSGGGK"
BASE COUNT         1179 a         2458 c         2467 g         1285 t
ORIGIN      
        1 tccgagaagg cgctgaagaa agccggcatg acgcccaaag acgtcgatct ctgggagatc
       61 aacgaggcgt tcgcagtcgt gccgctgcag accgcgcgca agctcaacat cgatctcgat
      121 cgcgtgaacg tgaacggcgg cgctatcgcc ctcggtcacc cgctcggcgg caccggcgcg
      181 atcctgctcg gcacggcgct cgacgagctc gagcgtgcga acaagtcgac ggccctgatc
      241 acgctgtgca tcggcggcgg catgggcatc gcgaccgtca tcgagcgcgt ctgatcgttc
      301 cgcacgacgc ccgcacgatt ctctaatgat acgttcctgc ggtgcgtcgc atcgccgtgc
      361 tgttcgcgct agccgcttgt agcgagccgg cgccgaagca ggctccacgg cgcaattggg
      421 tgtatccgcc ggcgcccgcg gcgtggcctc tgggatcggt gaccgcgacg atcgggcgat
      481 cgcaagcgcc ggacatcgcg ctcatcgaag ggatcgaggc ggggccgaac gccgggccgc
      541 tcacgccatt gcgcatgccg acgccgtggc ctgtgcccga cggtggcgca gccggcgctc
      601 acgcgcgcgc ggtgatctac ggaacgctcg gcgcccagca tgtcgtcgag ctgatcgaca
      661 tcgatgcggg caggctcctc tggcgcgata cgtccgcatg tcgcgcgccc gtcgtcgccg
      721 tgacggccga ggctgtcgta tgtgcggatg cgaagggcgt gcgggcgatc gcgatcgcgg
      781 acggcaagca gcgctggaag tccgacgcga cgttcatcgc gatgaccgac gatcgcgtga
      841 tcacggcggg cgccggcgaa tccgtgatcg tcgatgcgaa cgacggcggc gagctcgcgc
      901 gcgtgaagct gccgccgagt gtgcagagcg attcgatcat cgcgagctgc ggtgatgcgg
      961 gtcgcgagct attcgcatac ggccaagacg gccgcctcgt gcatgtcatc gaggcgaaag
     1021 gcggaccgac gattcagtgg tcgcttgccg tcgagtcgtt cacgacgatc gatgcatgtg
     1081 aagggccatc ggtgatcgtc gcgagtggca catcgctgct cgcggtcgca cgtgcgacgg
     1141 gtgtcgtcag cggatcgatc gacggcgtac gcggctattg gccggcgcgc gacggtagcg
     1201 acaagctcga ggtctcgacg agcaccggcg ttgcgttgcg cgctcgcgat ctgcgcgggc
     1261 cgagcgagtc ggtcgggttg ccgccactcg gcgagctcct aagcaagcgc ggcgacatgc
     1321 gcctggttcg cgcgacacca tcgacggcgg tcgtcctcga ctcgaaaggc gtgcgcgcgt
     1381 atctgccgct cgcgcagctc ggtgcggtgc tcggtgacaa cgcgatcatc gcgggaagct
     1441 ggacgggctc gcccggcgaa acggtgcggc gcatcgcgat tcccgatggg cgatatcgca
     1501 agcggctccg gctgccgcgc gtcggacgcg gcctcggcgt cacggcagag cttcgcgact
     1561 tgcccgccgc gcaagacatc gatgcgagcg gtgcgatcgc gaagaacgat gccggcaaag
     1621 ccgcagtcgc ggcggtcgcg ctcgatccgt cgggagccgc cgtgtacgcg acagcgctcg
     1681 atggcgagcc cgacgagaat tcgagtgctg gcgtcgcgcg cttcgatctc ggatcgcgca
     1741 cgtggacctg gtatcgcgca gacggctgcg gttcgggcgc gccgatcgcg ctggccgcgt
     1801 cgaacgaggt catcgtttgt ggcgcgcgtg ccggaagcac caccgcgccg gcacgcgtgg
     1861 gcggtggcac cgcagcgaaa ggcgacggcg gccgcgcgag tgttcgggcg acgacgcgtg
     1921 acgggaaatc ggcgtggaca tggcacagcg acaacgtcga cggcctcgat gcaatccgcg
     1981 cgctcgtgct cgtgcgcgat gcggcgcgcg tccacgtgct cgacgcggcc ggcggccatc
     2041 tgctcggcga cttcgagagc gacgatggca acgcgatgcc cgcggcgatc gtaagcgtcg
     2101 actttgatgc gacgtccgcg agtcgaggaa gcgatgcgac gtccgcaagt cgcggaagcg
     2161 attcggtgtc ggcgaagcat ggcgattcca tggagatgct cgtcgtcgcc gaacacgggc
     2221 gcgtcgtcgg acgcttgccc cgcgcgcaga tggtgccggc gtggtcgatc gcggttaacg
     2281 gcgtcgtgaa gctcatcgtt gccgccggcg acggcgtgct cgtcgagctc gaggacggcg
     2341 acgcgtatcg catcgacgcg cgaacgggtg cgaccatgcc ggtcgcggga atcggcctcg
     2401 actggcacgc gagcgacgac ctcttggcgg gcgacacgcc tggcgggccc gtcccaccgc
     2461 aggacatgct cgatcgcgtc ccgacgtcgg acgagccgcc agccgcaccc gcacccgcga
     2521 aaaagagcag gaagaaagca ggcaagaaag acgacaagcc caaagaggtc gtcgatgagt
     2581 cgctcggcat tccgccgcgc ctgccgcccg tctggcccga tccgggacca ttgcccgcga
     2641 gctggcagct gacgatttat gagccgaatg gtgcgttccg cgcgcgcaac gattacgcgc
     2701 tcgaagcgcc tatcactccc gcgaccgcgc gctcggcgaa ggctccgatc gtcgtgcaat
     2761 ccgggccgga acttcgcgac attctgcttc tcgatcccgg caccggcgat ccgctacgcc
     2821 gcgttcggct gccggaggac gctgctcccg ggacggcgtt ttcgacgatc gtcgacggga
     2881 aggctgtggt cggagtgttg ctcgcgaacc cgctgcgcgt ggtggtgttt tagaggcgtt
     2941 tcgctgatat agatcgaggg atggcactcc cgctccgtgc agcgctgcgt tccgctggcc
     3001 gcttcatcgc caagaatccg accaccatgt tgagcatggc gcgccacgca ctcggcatgc
     3061 ggctcgctgt gccgctcgat gcagtgcgct ggtttattgc gaacacgccg ccgtcgaaga
     3121 aggcgccgca ggacgtgacg atcacgccac ggccgcccgc gatcgggctt ggtgcgacgg
     3181 tggatctgat ggggacgacc gttcgcgcgg gcgcgtcggt gcgcatcgat cagatcgaag
     3241 tcggcgacga gaagctgcgc gtcacgctcc gcatttcgga tgtgaccatg aaggtgctcg
     3301 gcgattcggc gacgcccgtc gccggtctca tcaagagcgg cgcgctcgat ctgagcaagc
     3361 cgggcaacct cgccaagttc atgccgaaga agtcgccggt tctcgtcgag gcgcatgacg
     3421 acatcgtcgt gctcgacctg atgcagaatc cgaagatcgc ggcgaacgat cgcgtgcgcg
     3481 gcgtgctcag cacgttgacg cccgtcgtgc aggtgtcggg gctctcgacc gagggtgact
     3541 tcctgatcat ccagctgcgc gcatcgccgt ttggtctgcc gcgtgcgatc aacgccgcgc
     3601 gccagctcgc tgccggctga gctccaccat ggccgagtcc gtcgcgttcg atgcatcggc
     3661 ggacaagcct gcgcggtatc gcgagctcct gccgcagctg cgttcgctcg tcgacggcga
     3721 gccggatctg atcgcgaacc tcgcgaacat cacggcggcg ttgcgcgagt gtttgccggt
     3781 cgcgagctgg atcgggtggt acgtcaagcg cggcgacgag ctcgtgctcg ggccatttca
     3841 aggcaaggtc gcgtgcgtgc gcatcgcgtt cggcaaaggc gtttgcggca cggcagcggc
     3901 ggaaaagcga acggtgatcg ttcctgacgt cgagaagttt cccggtcaca tcgcatgcga
     3961 tgcggggtcg cgcagcgaga tcgtgctgcc gatcgtgcgt cgcggtgagg tcgtcgcggt
     4021 gctcgatctc gacagctacg agctcgccgc attcgacgac gtcgatgccg aatggctcgc
     4081 gcaggtcgcg gagctcgcga gcacgtttgc atggtgagta ggcttgcgct catcgcggct
     4141 ctcctcgccg cgggttgcca ggactcgggc acgccgaagc agaccgcgcg caaggatgcc
     4201 ccgctgaccg cgagcaccga cgacgcggat cccaccgacg cgccacctcc cgccgacgag
     4261 ccgacggagc ccgcaccaga tccaggcaag ctcgtcgcgg acctcggcgc gattcccgcg
     4321 tggcaagccg tggtcgatcg cggggagtac ctgcagcgac gcggtcagca cggcatcgtc
     4381 tacggcacgc tcggccccac gatccagatc gcggatcccg cgagcgacgg tggcctgatc
     4441 aattcgccgt acacctggct cgtcgacgac accgaaggca acggctgtct cgcgattcgc
     4501 gtcgcgctcg gcaacaagac accgaagctc ggcgatcgcg ttgcgcttgc cggcgcgtgg
     4561 aagctcgacg acaagctcga atatttctgg gacgtcgccg agctcacgca gcttccgccc
     4621 ggaccaccga gcgatctcaa ggatccgccc tcgccgcccg gacacgcgct gcaagaaggc
     4681 ccgctgccgc ccggcacccg catgatctcg ctcgcgaagg acaacgacct cgtctacttc
     4741 caggtcaccg gccgcgtacc actcgtcgac ggtgatggct ggcctgtcgc cgatcagctc
     4801 ggcaacaccg tcgtcgcgct gctcaacttg ccgggcgagc ggccgagctt cggcgcgcaa
     4861 gacttccgca cgaagaacga acgctggcag ctcaagcgcg gcatcacgta cttcgttcgc
     4921 atcggcaagg tccgcaagaa caaggatccg ctgaagccgg cgaacatcaa tgcgcgcacc
     4981 gcacccgtgc ggatgaaata gctacttctt ccagtcgcgg ccttcgcgtt tcgcgcggtc
     5041 gcggaggtct cgtacgagcg cgtagtacgc ctccggcgga ttctcgagga tcgcgcgatg
     5101 cgaatcgggg aatgacgtct cgaattcctc gagcttttgc atgcagccgt tgaccggctt
     5161 gcgtagctcg acacccgcga agcagcctga cgcgtactgc ttgtagcgcg cttcgtccca
     5221 cgcgtgcagc tgatcgtagg agaacgtcat cgacacgggc ggcgtcgtgt tcagctctgc
     5281 ttcgaagcga tacgtgaagt agtccgcgcg acgcagtccg ctgatcggtt tgtccgcctc
     5341 ggcgtagaga tacgcgcgac cacgcgtagg cgacagcacg gcgatgccga acgctttctt
     5401 ctcgtagctc ttcgtgcagt agaagttgat gacgtagacc ttcgcgttgc gatacgcgca
     5461 cgcgtagaca tggaacatgc cggtccactc gccggtgccg acgtagacga gcggccccga
     5521 tgcgatatcg gcgaagacct cctcgtacgt gtacagcgac gtatccggtt gctcgacgac
     5581 gaccggcgcg tccggcttca ccggctgctc gatcggcgtc atgcgcggcg gcggcgtgct
     5641 gtggtagcag ccagcggcca gcagcgcggc gacgcagcta gaagcgccag cccacacgcg
     5701 cagccacgcc gccgtttgtc ggctgcactt ccatgtccgg cagcacgcgc ttcgggcgat
     5761 tcatgatcgc ccacacgacg ccaccgatgg cgagaccgcc acccgcgacc atcatcgaca
     5821 ccgcgatctt gcctttgagc tccgcgctgt cgcgatcgcg cgcgaggtct ggctgatcgc
     5881 tcaagtcggc gttgcagccc gtcgggcagc gcatcgcgaa gtccgcctcg aactgatcca
     5941 tctgattgcg gcccgcgaac cagaagccga ggccacccag cccgatcgct gcgccacctc
     6001 cggcgacggt ccacgggatc cagcgcggcg tcgagtactc gagcttcacg accgattcga
     6061 tcggcaagag cgcgatcttc tcgctcgccg tgccgccacc cgcaacgacg acgcgacggc
     6121 tgaccgtcat gtacgccttc ttctcgccga cgatcgcgtg ctcaccggag aggacgcgct
     6181 gcttcttgct gccgggacac gagaaccacg gcttgccgtc gaggagaatt tgcgcctcgc
     6241 tctgatcgca ggtcgcctcc accgtgccta cgcggccgct gacgagcttc tggtaatcga
     6301 gcgcctgctg atacagctcg ggcgtgaacg gctgctggcc gaagcgaagc gcgctgtcga
     6361 gatcttcggc ggcctcgagg atcttgtcga ggcggatcag cgtcaccgcc atgttgaagc
     6421 ggatgagcgg atgatcccag agctggatcg cggcgttgta tttctcgagt gccggttggt
     6481 gtgcttgctg cgcgaagaac gtattgcctt cgcggaacag cgcttcggct tggtcgcgct
     6541 tgtctttcgg aacgccctgc tcccacggcg tctccgcgaa ggcggtagcc gagagcagca
     6601 tcaatacgac aatcgctttc ttcatttacc gcctccggaa gacaacgacc cgttgaagat
     6661 cttagacgat tcagcgcgca ggcgttcgtt ctcggccttc tgcttttcga gcatccgctc
     6721 gacctgcgtc ttcgcgacct tcgcgtcttc ggtcgccttc ttcgcggcag cttccgaggc
     6781 gagcgccttt tcctttgccg cctgcgcctc tgccaccttt cgctgaagct ctttgttcgc
     6841 ttcggcgagc tgctctttcg tcatctccgt cgacttcgtt gcgtcctcgg cgagcttctc
     6901 ggcggcgagg cgctgcttct tctcggcttc gatgatgtcg agcttgcctt gcaggtcgcc
     6961 cttcgccttg accgcttctt cggtcgcttg cttggcctgc cccagcgcgc cttcggcttc
     7021 cttcttcgcc tttatcgctt gtgcagcctt atcctgcgcg tccttctcgg cgagcttgat
     7081 gcgaacgagc gcgaagctgc cgcctgcgag aacgagggcg agtgtcactg cgatggccgt
     7141 gaacacggcg agcttgcgac gccggctgcg agccgtttgc gccttcacct ccgccaggaa
     7201 ctcgcgctcc acggtcgaga gatccaagac gtggcgcttc acgtggccga gggcctcttg
     7261 cgcggttgcg ccacgccaca cgaggtccgc gggcttgccg cgcgacgccc actgcttcgc
     7321 cgcctgccgc agctcgtgca tgaagccgcg catcgcttgg ctgtcttcga gccaacgctt
     7381 gagcgtcgg
//
LOCUS       DATLBZ010000162         7326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9766300, whole genome shotgun sequence.
ACCESSION   DATLBZ010000162 DATLBZ010000000
VERSION     DATLBZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9766300"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VMZ53_08545"
     CDS             complement(<1..893)
                     /locus_tag="VMZ53_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HUS28544.1"
                     /translation="MTTTTTLPPALRAPFSSGGTAAIDAQLANRLLGLALEAGGDYAD
                     LYFEFRVSADYSLEEEQVRTLGRGITLGLGVRVTKGDATGYAYTEDLSWEKMAHAAKT
                     AGQIATAGGAKPFSVDMQNIPLPAFYKVPTPSLMMPPADKLALLRAADKAARAFDSRI
                     VKVQVSFVESIREVLVFTSDGRMATDIQPILRFGVSAVAEDNGKRQGGSSGGGGRYGM
                     EYFTQAARDAAAHGKEASRIAIAMLDAVEAPAGEMTVVLGSGDSGILLHEAVGHGLEA
                     DFNRKQTSNYSGQIGKKVASDL"
     gene            978..1733
                     /locus_tag="VMZ53_08550"
     CDS             978..1733
                     /locus_tag="VMZ53_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUS28545.1"
                     /translation="MPFTVGDLAKLTGITVRTLHHYDEIGLVRPSERSAAGYRLYSDA
                     DVHRLQQVLLFRELGLPLEDIASAIDSGRGVDPAKRAALLREHREALLAKRARLDAMV
                     SAVDAALGTLEKGHAMQPDDVKDMFDGFDPSQYEEEVKERWGNTDAYKESARRTKSYT
                     KEQWQQLKDQAAEIYAALADLMRAGVAVTDARVQTLVEAHRAHIDRWFYPCSKELHKG
                     LGAMYVADPRFAENLNKNGDGFAQYLSDAIAAS"
     gene            complement(1751..2374)
                     /locus_tag="VMZ53_08555"
     CDS             complement(1751..2374)
                     /locus_tag="VMZ53_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28546.1"
                     /translation="MELRALTILDSLQPQLTGFLQTVCAGFMPLEQEAAAFIEIAPGI
                     AINQLTDAALKATTVRPGMQIVERAYGMLEFHDRDKGQVEAAIDAIIKQMGVKREDRI
                     KPRIVSSQIITGMDGHQSQLINRMRHGDMIMAGQTLYILEVYPAAYAAIAANEAEKAA
                     DIHLMEVITFGAFGRLWLGGWEAEVKAAAAAAESELAKIAGREDKKS"
     gene            2409..3179
                     /locus_tag="VMZ53_08560"
     CDS             2409..3179
                     /locus_tag="VMZ53_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUS28547.1"
                     /translation="MRRLAVLVLLIGCGEDSAATTDASATSDDAGDAVPDAAGPCPAG
                     MAHITATSGAFCIDRHEGALEERASDGTWSPASPFLTVGTRTVRAVPAAGITPQGYIS
                     GVEAKAACAASGKHLCTSAEWLAACRGSANNVYPYGATHVAGACNDAYSGHPVVNYFG
                     TSNGVFDSQHMNDPGINQQPDSLAKGGAFTQCTTPDGVFDMHGNLHEWIDDAAGTFRG
                     GFYADASLNGPGCTYATTAHAFSYHDYSTGFRCCAGAN"
     gene            complement(3176..4990)
                     /locus_tag="VMZ53_08565"
     CDS             complement(3176..4990)
                     /locus_tag="VMZ53_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28548.1"
                     /translation="MKAFASTASPRTAAIKIVAVYWALLVFGMVLYTAMRLDALAILE
                     PMWAGTIAGTALGQVLAVRRYRTWLAVLLIIAVALFILPHAPPELGGKRLWFAFLPAA
                     MCGYWSLGDRTSLASFWFPAVIWMLSILDGAHAGAMPNGNGLVLFAGLAVLFVVFLRV
                     REERRVALWRSVAPEPLASPLPMLVVKERAGMQLARGAWGVFVTGLGFAATAWLAPTL
                     WKPETHDGKHVAVGAESPNQIGVPCCPAVAEDEPTHTRVKEYFNIGRADTEHHVSEPK
                     DCRVCPEEETYASNEYAGFTRPSMVGDVHYHDGVPCDGTGHYHDVPAVDGYGTYTPTV
                     RIGSYQPPSGTGGYDGSSPSTTGAGTPSSPGTANPWATPSQWQNPSTATPNANGSPYP
                     SGVSITTNVPAPAAPTPSSYDYERPSQPTPPAAQPHHPAAAPTPARPHLASNTSPAVT
                     HNGPSTPVRPDRVGGFELLHWVAVLIAALFFVQLAALLLRPLRRALVLRHLRRPFWSE
                     TVDQRVSNAWQLVLVGLRDAGWRADGGEAPREVAKRVQVDGVEDCATILDRARHGIRI
                     DREDLDKMVASADGAYRGARTKVGRFARAISWLRWPLV"
     gene            complement(4987..6579)
                     /locus_tag="VMZ53_08570"
     CDS             complement(4987..6579)
                     /locus_tag="VMZ53_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUS28549.1"
                     /translation="MMTPPGTWAPSDRVMSAASARRLRIASLLAPMFIVTVLVADDAG
                     GAMMHAAGFALYGIWLALVVVLGVRFVIGCRRKAADSSATAPWDHVDVLTSSGTAMLW
                     TGVAALVLARVTGWASFSVLGVLGVGVVLLAALWTACAAVGERPWRRAKVTRSVTPAI
                     AVEGEMLREQITIADLDVPPGMRLFVLGRAMRHGAPSRYVVDGKDACGEVKLEAELGA
                     ALRGEHQVPPLMMWFADVLGLARSGVVYRGETTFAVKPRAAAVDGVQKLLGAGGDAQI
                     ARQTVHAPTDGTFRIREYVPGDDARRVHWVRAAQRDDLVVRLPDEIPPAEPSIRLVLD
                     NELALCEALNCRAADELLDALVRIWLGIGKALTANGTRVTLVVAAEKNGAMKLVERRM
                     DSRATDVVAQLGSRVAWQNGLTLESMLDKSAERQIVVSARPRRVGTEHVRWVVVPEVA
                     WTSLDVDTVTEDTFEHPFPVGSPENRRTRRHRERHTAMVKWQDRAFFSQMVCWSEWSR
                     YAGDHVARFDRGRGRVIVEVIP"
     gene            complement(6576..>7326)
                     /locus_tag="VMZ53_08575"
     CDS             complement(6576..>7326)
                     /locus_tag="VMZ53_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS28550.1"
                     /translation="ITAAIQASFGRIQFTPDLLPMDITGANIFDMKDREFRFRPGPIF
                     THILLADEINRATPKTQSALLEVMEERSVTIDGVTHRLAEPFQVLATMNPLDHQGTYA
                     LPAAQIDRFMIMIEIGYPTPEDEVRVLDYHLGATSPLSAVQPVISRADLLAWHETVPM
                     IHVTPELKRTVVEYVTRLRRTAEDGMALSPRATLAWVRAAQSRAMLSGREFVTIEDVL
                     DVSPDVLRHRLWTDGATVRERLRAVAIAGAR"
BASE COUNT         1301 a         2535 c         2333 g         1157 t
ORIGIN      
        1 aggtcactcg cgaccttctt gccgatctgg cccgagtagt tgctcgtctg cttgcgattg
       61 aagtctgcct cgagaccgtg accgacagcc tcgtgcagca ggatgcccga gtcaccggag
      121 cccagcacga cggtcatctc gccggccggt gcttcgacgg catcgagcat cgcgatcgca
      181 atgcgcgacg cttccttgcc atgcgccgct gcatcacgcg cagcttgcgt gaagtactcc
      241 atgccgtagc gcccgccgcc accgctcgag ccgccctgac gcttgccgtt gtcctcggcg
      301 accgcgctca cgccgaagcg caagatcggc tggatgtccg tggccatgcg gccgtcgctc
      361 gtgaagacga ggacctcgcg aatcgattcg acgaagctga cctgcacctt gacgatgcgc
      421 gaatcaaacg cgcgcgccgc tttgtccgca gcacgcaaca gcgcgagctt gtcagccggc
      481 ggcatcatca gcgacggtgt cggcaccttg tagaacgccg gcagcgggat gttctgcatg
      541 tcgacggaga acggcttcgc gccgcccgcc gtcgcgatct gtcccgcggt cttcgcggcg
      601 tgggccatct tctcccacga cagatcctcg gtgtacgcgt agccggttgc gtcgcccttc
      661 gtgacgcgca cgccgagccc gagcgtgatg ccgcgcccca gcgttcgcac ctgctcttct
      721 tcgagcgagt agtccgcgga cacgcggaac tcgaagtaca agtcggcgta atcgccgccg
      781 gcttcgagcg cgagaccgag caatcggttc gcgagctgag catcgatcgc tgccgttccc
      841 cccgaggaaa acggtgcccg caacgcgggc ggaagcgtcg tcgtcgtcgt catttgccga
      901 ccatagcgcg agttctgcga aaaagcgctt gaccctcccg cgacgtgagg ctgcaaggtg
      961 acagcgagag tgacgccatg ccctttacag tcggagacct cgccaaacta acgggtatca
     1021 ccgtccgcac actgcaccac tacgacgaga tcggtctcgt tcgtccgtcg gagcggtcgg
     1081 cggccggcta tcggctctat tccgacgccg acgtccaccg cctgcagcaa gtgctgctat
     1141 ttcgcgagct cggcctgcca ctcgaagaca tcgcctcggc gatcgactcg ggacgcggcg
     1201 tcgatccggc gaaacgcgca gcgctgttgc gggagcaccg cgaggcgctc ctcgccaaac
     1261 gcgcacgcct ggacgcgatg gtctccgccg tcgacgcagc actgggcact ctcgagaaag
     1321 gacacgccat gcagcccgat gatgtgaaag acatgttcga tggtttcgat ccgtcgcagt
     1381 acgaagagga agtcaaagag cgctggggca acaccgacgc gtacaaagaa tcggcgcgcc
     1441 gcacgaagag ctacaccaaa gagcagtggc agcagctcaa agatcaggcg gccgaaatct
     1501 acgccgccct cgccgatcta atgcgcgcag gagtcgcggt caccgatgca cgcgtgcaaa
     1561 cgctcgtcga ggcgcatcgc gcgcatatcg atcgctggtt ctacccgtgc agcaaagagc
     1621 tgcacaaagg cctcggcgcg atgtacgtcg ccgatcctcg gttcgccgag aatctaaata
     1681 agaacggcga cgggttcgcc cagtacctca gcgacgcgat tgcggcgagc taacgcagag
     1741 cgcgacgcgc ctagctcttc ttgtcttcgc gaccggcgat cttggcgagc tcgctctcgg
     1801 cggcggcggc agcagctttc acttcggctt cccaaccgcc gagccacagg cggccgaacg
     1861 cgccgaacgt gatgacttcc atcaggtgga tgtcggcggc cttctccgct tcgttcgcgg
     1921 cgatcgcggc gtacgcggcc ggatacacct cgaggatgta gagcgtctgg ccggccatga
     1981 tcatgtcgcc gtggcgcatg cggttgatca gctgtgactg atggccgtcc atgcccgtga
     2041 tgatctggct cgagacaatg cgcggcttga tgcggtcttc gcgcttcacg cccatctgct
     2101 tgatgatcgc gtcgatggcg gcctcgacct gtcccttatc gcgatcgtga aactcgagca
     2161 ttccgtacgc acgctcgacg atctgcatgc ctggccgcac ggtcgtcgct ttgagcgcgg
     2221 catcggtgag ctgattgatc gcgatgccgg gcgcaatctc gatgaacgcg gcagcttcct
     2281 gctcgagcgg catgaagcca gcacagacgg tttgcagaaa ccccgtcagt tgcggctgca
     2341 gcgaatcgag gatggtgagc gcgcgaagtt ccatttcggc gaaacgctat cacggttatg
     2401 gtcgggccgt gcgcaggctc gcggtgctcg ttctgctgat cggttgtggc gaggattccg
     2461 ccgcgacgac cgatgcgtcg gcgacgagcg atgacgcagg cgatgccgtg cctgatgccg
     2521 ccggtccgtg ccccgccggc atggcgcaca tcaccgcaac gtccggcgcg ttctgcatcg
     2581 atcgtcacga aggcgcgctc gaagagcgag catccgatgg cacgtggtcg cccgcatcgc
     2641 cattcctgac ggtcggcacg cgcacggttc gcgcagttcc cgctgccggc atcacgccgc
     2701 aaggctacat ctccggcgtc gaagcaaagg cggcgtgcgc agcatcgggc aagcacctct
     2761 gcacgagtgc ggagtggctc gccgcgtgtc gcggatcggc aaacaacgtc tacccatacg
     2821 gcgcgacgca cgtcgcgggc gcgtgcaacg acgcgtacag cggccatccg gtcgtcaatt
     2881 acttcggcac atcgaacggc gtgttcgact cgcagcacat gaatgatccg ggcatcaatc
     2941 agcagcccga ttcgctcgcc aaaggcggcg cattcacgca gtgcacgacg cccgacggcg
     3001 tgttcgacat gcacggcaac ctgcacgaat ggatcgacga cgccgcaggc acgttccgcg
     3061 gcggcttcta cgccgacgcc tcactcaacg ggcccggctg cacgtacgcc acgacggcgc
     3121 acgccttcag ctaccacgac tactcgacgg gctttcgctg ctgcgcgggc gcgaactaga
     3181 ccagcggcca gcgcagccac gagatcgcgc gcgcgaagcg accgaccttg gtacgtgcgc
     3241 cgcgatacgc gccgtccgcg gaagcgacca tcttgtcgag gtcctcgcga tcgatgcgaa
     3301 taccgtggcg cgcgcgatcg aggatcgtcg cgcagtcttc gacgccgtcg acctgcacgc
     3361 gcttcgcgac ttcgcgcggc gcttcgccac cgtcggcgcg ccagcccgcg tcgcgtaggc
     3421 caacgaggac gagctgccat gcgttcgaga cgcgctgatc gacggtctcc gaccagaacg
     3481 gacggcgcag gtggcgcagc acgagcgcac gacggagcgg gcgcagtagc agcgcggcga
     3541 gctgcacgaa gaacagcgcg gcgatgagga cggcgaccca gtgcagcagc tcgaagccgc
     3601 ccacgcgatc ggggcgaaca ggcgtcgatg gaccgttgtg cgtcacggcg ggcgacgtat
     3661 tcgacgcgag gtgcgggcgc gccggcgtcg gggcagcggc cgggtgatgc ggctgtgctg
     3721 caggcggcgt cggttgcgac gggcgctcgt agtcgtaact cgagggcgtc ggagcggcgg
     3781 gcgcaggtac gttcgtcgtg atcgaaacgc ccgagggata cggcgagcca ttcgcgttcg
     3841 gtgtcgccgt cgacgggttt tgccactgcg atggtgttgc ccacgggttc gcggtgcccg
     3901 gagagctcgg cgttcccgcg cccgtcgtcg atggcgacga gccatcatag ccacccgtgc
     3961 cggaaggcgg ctggtacgag ccgatgcgca ccgtcggcgt gtacgtgccg tagccgtcga
     4021 ctgccggaac gtcgtgatag tggccggtgc cgtcgcacgg aacgccgtcg tgatagtgca
     4081 cgtcgccgac catgctgggc cgggtgaagc cggcgtactc gttcgacgcg tacgtctctt
     4141 cctccgggca cacgcggcag tctttcggct cgctgacgtg atgctcggtg tccgcacgac
     4201 cgatgttgaa gtactccttc acgcgggtgt gcgtgggctc gtcctcggcg actgctgggc
     4261 agcacggcac gccgatctga ttgggcgact ctgcgccgac agcgacatgc ttgccgtcgt
     4321 gcgtctccgg tttccacaac gtcggtgcga gccacgcggt cgcggcgaag ccaagcccgg
     4381 tgacgaacac gccccacgcg ccacgcgcga gctgcatgcc tgcgcgctct ttgacgacga
     4441 gcatcggtag cggcgatgca agcggctccg gcgcgacgga gcgccacagt gcgacgcgac
     4501 gctcttcacg tacgcgcagg aacacgacga acagaaccgc aaggccggcg aacagtacga
     4561 ggccattgcc gttcggcatc gcgccggcgt gcgcaccgtc gaggatcgac agcatccaga
     4621 tgacggccgg gaaccagaac gaggcgagcg acgtgcgatc gccgaggctc cagtagccgc
     4681 acatcgcggc cggcaggaat gcgaaccaca ggcgtttacc gccgagctcg ggcggcgcgt
     4741 gcggcaggat gaacagcgcg actgcgatga tgagcagcac ggcgagccac gtgcgatacc
     4801 gacgaaccgc gagcacctgg ccgagcgcag tgcccgcgat cgtgcctgcc cacatcggct
     4861 cgaggatcgc gagcgcatcg aggcgcatcg cggtgtagag caccatgccg aacacgagca
     4921 gcgcccagta gaccgcaacg atcttgatcg cggccgtacg tggcgatgca gtggatgcga
     4981 atgccttcat ggaatcacct ccacgatcac gcggccgcgt ccgcgatcga atcgcgcgac
     5041 gtgatcaccc gcgtaccgcg accactcact ccagcagacc atctgactga agaacgcgcg
     5101 gtcttgccac ttcaccatcg cggtatgccg ctcgcgatga cgacgcgtcc gtcgattctc
     5161 cggcgaacca accgggaatg gatgctcgaa cgtatcctcg gtgaccgtgt cgacgtcgag
     5221 cgaggtccac gcgacctcag gcacgacgac ccagcgcacg tgctcggttc ccacgcgacg
     5281 aggccgcgcc gatacgacga tctgccgctc ggccgacttg tccaacatgc tctcgagcgt
     5341 gagcccgttc tgccaggcca cgcgcgagcc gagctgcgcg accacatccg tcgcgcgcga
     5401 atccatccgc cgctcgacga gcttcatggc gccgttcttc tccgccgcaa cgacgagcgt
     5461 cacgcgcgtg ccgttggccg tcagcgcctt gccgatgccg agccaaatgc gcacgagcgc
     5521 atcgaggagc tcgtcggcgg cgcggcagtt gagcgcttcg caaagcgcga gctcgttgtc
     5581 gagcaccaag cgaatcgagg gctcggccgg cggaatctca tcaggcaagc gcacgacgag
     5641 gtcatccctc tgcgcagcgc gcacccaatg gacgcggcgc gcgtcgtcgc cggggacgta
     5701 ctcgcgaatg cggaacgtgc cgtcggtcgg cgcatgcacg gtctgccgcg caatttgcgc
     5761 gtcgccgccg gcaccgagca gcttctgcac gccatcgacc gccgcagccc tcggcttcac
     5821 ggcgaacgtt gtctcgccgc gatacaccac gccgctgcgc gccaggccga gcacatctgc
     5881 gaaccacatc atgagcggcg gcacttgatg ctcaccgcgc agggccgcgc cgagctcggc
     5941 ctcgagcttc acctcgccgc atgcatcctt gccgtcgacg acataacgcg acggcgctcc
     6001 atgccgcatc gcgcggccca gcacgaagag ccgcattccc ggcggcacat cgagatccgc
     6061 gatcgtgatc tgctcgcgca gcatctcgcc ttcaaccgca atcgccggtg taaccgatcg
     6121 cgtcaccttc gcgcgtcgcc acggtctctc gccgaccgcc gcgcacgctg tccacagcgc
     6181 ggccagcaat acgacgccga caccgagcac gccaagtacc gagaagctcg cccaccccgt
     6241 cacccgcgcg agcaccagcg cagctacgcc cgtccacagc atcgcggtgc cactcgacgt
     6301 cagcacgtcg acgtgatccc acggcgccgt tgccgacgag tcggccgcct tcctgcggca
     6361 accgatcaca aagcgcacgc cgagcacgac gaccagcgcg agccagatgc catacagcgc
     6421 gaagcccgct gcatgcatca tcgcgccgcc agcatcatcg gcgacgagca ccgttacgat
     6481 gaacatcggc gcgagcaagc tcgcaatgcg caaccgcctc gccgatgccg cgctcatcac
     6541 gcgatcgctc ggcgcccacg ttcccggtgg cgtcatcatc gcgcccccgc aatcgcaacc
     6601 gcacgcagcc tctcgcgcac ggtcgctccg tctgtccata gccggtgtcg caacacatcg
     6661 ggcgacacgt cgaggacgtc ctcgatcgtc acgaactcgc ggcccgagag catcgcgcgc
     6721 gattgcgccg ccctcaccca cgcaagcgtc gcgcgcgggc tgagcgccat cccgtcctcc
     6781 gcagtgcgcc gcaaccgcgt tacgtactcg acgacggtcc gcttcagctc gggcgtcacg
     6841 tggatcatcg gcaccgtctc gtgccacgcg agcaaatccg cgcgcgagat cacgggctgc
     6901 acggccgaca gcggcgacgt cgcgcccaag tgatagtcga gcacgcgcac ttcatcttcg
     6961 ggcgtcggat acccgatctc gatcatgatc atgaagcgat cgatctgcgc cgcaggcagc
     7021 gcatacgtgc cctgatgatc gagcggattc atcgtcgcca gcacctggaa cggttcggcg
     7081 agtcgatgcg tcacgccatc gatcgtcacg gaccgctcct ccatcacttc gagcagcgcc
     7141 gactgcgtct tcggcgtcgc acggttgatc tcatccgcga gcaagatgtg cgtgaagatc
     7201 gggcccgggc gaaaccggaa ctcgcgatcc ttcatatcga agatgttcgc gcccgtgatg
     7261 tccatcggca gcaagtcagg cgtgaactga atgcgcccga agctcgcctg aatcgcggca
     7321 gtgatc
//
LOCUS       DATLBZ010000163        20265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_898969, whole genome shotgun sequence.
ACCESSION   DATLBZ010000163 DATLBZ010000000
VERSION     DATLBZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20265
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898969"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VMZ53_08580"
     CDS             complement(<1..988)
                     /locus_tag="VMZ53_08580"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS28551.1"
                     /translation="MKTRVAPAALILAALATLLLWRCHDRDSSRSKSSTKAAQKSVGS
                     GATMSTQERPDPKTQPRGSIAGTITDEQKAPIAGARVCAELYSQQISAQEVRDPICVT
                     ANDKGQYTLPNLFAAEYEVAASAKTFRPGSYHPSGPHDPTTFELKAGEAKTGIDIALR
                     SGGVEITGIVSDVSGGPIAHALVTASRDWGDGDLMPPTETDEAGAFSLWVKPGDVTVN
                     ATADGYAPGSDNGQAPGAFQVLLTPESSLAGTVVDAKSGEPVPGVVVEAEGGEWGSFS
                     FFGGGSGQNTDVTDDKGAFRIARLDPGRYTVNATSAHGYGVSAGSTLVGLGQH"
     gene            complement(1081..1947)
                     /gene="panC"
                     /locus_tag="VMZ53_08585"
     CDS             complement(1081..1947)
                     /gene="panC"
                     /locus_tag="VMZ53_08585"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HUS28552.1"
                     /translation="MTFARPPFEIIRDPKAMRARVEDLRRDGRRIAVVPTMGALHEGH
                     LTLLRAARERADIVILTIFVNPTQFGPNEDLSRYPRDEAGDIAKARTCGIDLAFCPDA
                     SAMYPAGAQTFVEVRELQKPLCGASRPGHFAGVATIVTKLFHITNPHLAVFGEKDYQQ
                     LAIIRRMVRDLDFGIEIVGVPIVREADGLALSSRNAYLSVEQRQAALSLSKGLAAAEA
                     AFKAGERDAAKLVGTARAQLEAAPGVRIDYLELRDADELSEIPRVEKRAVMAVAAFVG
                     TTRLIDNRVLAP"
     gene            complement(1957..2931)
                     /locus_tag="VMZ53_08590"
     CDS             complement(1957..2931)
                     /locus_tag="VMZ53_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28553.1"
                     /translation="MAATARPPGIGDARMPCPLCGGLIHPVAGRCKHCKQDLTSFRAG
                     RPQAAATLPSLNGHAISAPAPSTLHTPASTTTNFAPPIITTTNGTNGHAPAIPLAVPQ
                     AREGSQPILPPRETGSWRAKETQSGSLWRSWPMLVIGVAVLAIVTAVVIMVLPQDTKK
                     AGKKIGAPPPAPERMDVDPTHPKSSQLTPPQGNDPWAQPGNPAAPAPQPQAPDPMTPD
                     PNDPNDLFGGLSGGAPIGAGGGAVMISAFGKMCTKLKSCGNTDPTMATVCDQFATLQR
                     QLPVAPPSCAAAQKCLQAIDNFSCTDDSVNVVTAVYTIQDCSTAMTDC"
     gene            3045..3791
                     /locus_tag="VMZ53_08595"
     CDS             3045..3791
                     /locus_tag="VMZ53_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUS28554.1"
                     /translation="MAPPPPRVSEHEVRIKNLDPRHDGVRIAQLSDIHVGNMTPASHV
                     RAAIDAANRAKPDLVFLTGDYVCWKRSEAELVGQQLRGLQAKRVLAVLGNHDYFTWGS
                     GVARALESCGYEVLKNQTTISDVNGAPLALVGVDDPVTRHDDFDAAFKGANGYTKLVL
                     CHAPDSGPALAERGADLVLSGHTHGGQIYFKGITDRLMRRIGLNYRRGMFTFGDTTLY
                     VTPGVGFSGIPRRIGEGTEAEVAVLTLRAA"
     gene            complement(3792..4628)
                     /locus_tag="VMZ53_08600"
     CDS             complement(3792..4628)
                     /locus_tag="VMZ53_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS28555.1"
                     /translation="MVMRALRVLVLVLVGCVDQPTFVGKGSPIECSNTTDPTNNTATF
                     DETCTGMMFGDHCYVYDYLAYTPWQPAVDACAMAGGYLVTTGSSAEDSFVVSLIGGNQ
                     TWLGARQMGGMTTWANSEPVSFTRWLSGGDGCAQEDPAGWGPTDCGAGYRSHVCETPG
                     WTKFQGHSYRHFFEPVTWMMAKARCQALGAHLATILSECEGDFVRELAPYASEFWLGA
                     ERMPAFTWVADGELWEYEAFAAGEGQARDEECLAVDGVTGKWNDVACSAARGYVCERD
                     PD"
     gene            complement(4622..5527)
                     /locus_tag="VMZ53_08605"
     CDS             complement(4622..5527)
                     /locus_tag="VMZ53_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28556.1"
                     /translation="MRSLVVVALLGGVAYAENDPVAGIEAAKRAEDLVKAGRHVEGAT
                     MFRSAYRLDPRPEYLCNIGVAYHRAKVLPKAQIYLAECLRRGKSMDASFLDLVRKSLD
                     ATEATLKSGDFTPLDIVVTPDTASLTVVDVLDRDETLVGSQLLWVPFGKVTIVVTATG
                     LEPQRREVEALTHTTRQVVFELVPPKPVEPTPIATRSEHRSRTPALVATLATAAVGVS
                     ALGAYIHARGIMSDAASTEITRSEYGAVVDRAHTWQHASWVMGGVSALGAGVSAWLWL
                     RSGTKTTIEVSTQNQATAFVLSGVW"
     gene            5628..7049
                     /locus_tag="VMZ53_08610"
     CDS             5628..7049
                     /locus_tag="VMZ53_08610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28557.1"
                     /translation="MVDTRTSMSGGTGAADEAYCATCGTSYAAGTVRCPQDGGTLVNF
                     RSTQDPWIGRVLDGRYEIRSAIGAGGMGAVYRGKQLTMDREVAIKVIKSTVVADREVA
                     KRFLREARLSSRLVSGNIVTVFDSGQTDDGSLYLVMELIDGKTLASVIAASSPITSRR
                     VASIGRQLCDALETAHELGIVHRDLKPHNVMVLSTALGRDQIKVLDFGLAKSITGDSM
                     SQITATHAMLGTPAYMAPEQIENGMIDGRTDLYAVGCILYEMLAGRPPFQAAQITALF
                     AMHLGEAPPPLPADVPPTLARTVMRLLEKSPDARFASAAELRAVLDEVSPATPVTGVP
                     VVSAAALSAARPVAPARRRGLVLGSALALVVALGVGAFVFVTRHTPPAAEPSAVKPTS
                     NVVAPEPPRTPPPPPIAVPSPAAVLVPAPVPAPVTTTPKTTKATKATRPAKHAKPKPD
                     APPTTTTKPVSPPDELPLAPIRR"
     gene            complement(7062..8690)
                     /locus_tag="VMZ53_08615"
     CDS             complement(7062..8690)
                     /locus_tag="VMZ53_08615"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28558.1"
                     /translation="MRVLAAALLFSVVACSSEEADKPPVLEVSSPARGTVADAETVTV
                     TGHVTDDHAGVKVTIAGTEVAVAADGSFSASVPVPPGIAIIETHAIDKGGNDVRDVRS
                     VLAGKLAPSDGTQAGQVGARAGTTALTAVANAVATTAKAIDYTAAVQPMNPVYDNGGC
                     LGATINITSVSLTDVDAALIPKTNLLATDVVLSNLVVKLSAKFKVACIGGSTTITVRA
                     SKTKIHGDLGARIASEKIMTSLANTNVTFDNFSVDVGGVPGEIESLLKGKARDGVSSA
                     LEKAIQAKVPPIADKALAGLLAKPLNTSILGSDAAISIAPTKVAISATDLFVGFDTKL
                     KVTGGEGGTYLTTPTTVTGMNATQNLGIALDDDIINALFAGLWAADAFDKSISIDSIP
                     ALGALLDDDARTIDVKLALPPTVSTAGGELEMSVGDLLVTVKDENGAVVHDMAVSLRT
                     MLQAQPTQAGHVTLSVGAPTVFAQVLANGDTVEEPLTDNQVEALITGVWGVIGVKADD
                     ALSKLPMPSLAGIQLGAPSIEAGDGFVLADVPVM"
     gene            complement(8752..10089)
                     /locus_tag="VMZ53_08620"
     CDS             complement(8752..10089)
                     /locus_tag="VMZ53_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HUS28559.1"
                     /translation="MTYAEWRDRARVLLANNVQPHEATFTPSLFEAPLTAPPSTLRVP
                     RRFVALAEDAILHRERPDALLYRALWRLMHGEPALVEDAADPDIRALSVMAGAVKRDL
                     HKMHAFVRFRLVSEHGDDHYVAWYAPDHQIVERAAPFFAERFAAMRWTILTPERSVAW
                     DGNELQFFAGVPRHAAPAADELEDLWRTYYASIFNPARANPRVMRQHMPARYWAAMPE
                     TALISDLLAAAAPRVETMTTHVTGKPLPVLRDEARHCSACELCGPATQTVFGEGPVDA
                     RIVLVGEQPGDQEDLAGRPFVGPAGAVLDAALARAGIARDTLYVTNAVKHFRFLPRGK
                     KRLHQRPTADQVRAYKPWLAAELAAIQPRVIVCLGATAALSLIGSRFSITRDRGRVVQ
                     TSWAETLLATHHPAAILRVDPADRPRYEKELAEDLRTAVLRGSFPSLATGVAP"
     gene            complement(10086..11291)
                     /locus_tag="VMZ53_08625"
     CDS             complement(10086..11291)
                     /locus_tag="VMZ53_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HUS28560.1"
                     /translation="MNVREKLAILADAAKYDASCASSGAKRSGTKTGLGHSTGNGICH
                     SYTPDGRCVSLLKILLTNKCTFDCTYCVNRRSSDIARAQFTPEEVVELTLGMYRRNYI
                     EGLFLSSGVLRDPDYTMELVTRVARTLRETHEFRGYIHLKAVAGASDALVAEAGRWCD
                     RLSVNIELPTPADLDKLAPEKRLPVIQQSMTTIAEHHADAPKRFAPAGQSTQMIIGAT
                     ETPDATILATSSQLYSEFKLRRVYYTGYSPTQRADARLPLQAPPLVREHRLYEADWLL
                     RHYGFAVDELTTTEQPNLDLEIDPKLAWALRNRARFPIDVNTADREELLRVPGLGVRN
                     VDRIVAMRRQRTVRLEDLARLRVVMKRASPFVITADHNPDSLLIDRLDLRKRVKSEQL
                     AFDFRTGQL"
     gene            11324..11506
                     /locus_tag="VMZ53_08630"
     CDS             11324..11506
                     /locus_tag="VMZ53_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28561.1"
                     /translation="MAPKKNSLVNNINRRKKAGKSRSKARSTVSEESYTEMRKGWTGS
                     KSGRRKPGRKAKKSHR"
     gene            complement(11497..12513)
                     /locus_tag="VMZ53_08635"
     CDS             complement(11497..12513)
                     /locus_tag="VMZ53_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HUS28562.1"
                     /translation="MREAQTLLRELCAKRAKIIVAPDRDVDGLAAGVLVMRAIERLGG
                     QPVIALPNKGEHVHTPSMRARLAGLQADALIVLDMGSRAGPIVEDLPTIVIDHHDARE
                     KPDGAIFVSAAGCEPVAPTGLLAYELLSEIVDLQDLGWLALLATHADLGDHHPFAEEL
                     AVPASQTKKTHVRETISLLNSARRAASYQPELALEVLLAAHGPEDIARRKLDGVAALE
                     ACREEVKAEVARVARVAPKVVGDVALIRFTSGAQIHPLIATRWAGRLAPKVVLVANDG
                     YLPGRVNFALRSASKRDLLGFLRDLPLGRVEGEFANGHPRATGGSIPPHEFDRMLGVL
                     GFQR"
     gene            12551..13480
                     /gene="uvsE"
                     /locus_tag="VMZ53_08640"
     CDS             12551..13480
                     /gene="uvsE"
                     /locus_tag="VMZ53_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HUS28563.1"
                     /translation="MVRLGLCCTFLAAPIRFRTTTVRYALSLDSRVRRGFLNELALHN
                     AAALTSAIEWCIDHDVGAFRVQSGLLPLYTHPEVGWTLDGEEGAGVRAALAAAGQRAR
                     DAGVRLSFHPDQFVVPGSTSKNVVKASLRELEYIAEVGELIGADQMTIHGGGAQGGKP
                     EALKRLLVGLADLSPRARSLVALENDDRTYHVEDLLPICEGSGIPLIYDVHHHRCNPD
                     SLSVQRATELAAKTWGLREPWVHLSSPLAGWRDGDPRPHADFIKSSDVPAFWLDRTMT
                     IDVEAKAKEKAVLRLKTWIDAQAARGKRTRSSA"
     gene            complement(13441..14205)
                     /locus_tag="VMZ53_08645"
     CDS             complement(13441..14205)
                     /locus_tag="VMZ53_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HUS28564.1"
                     /translation="MTIKLITIPFSHYCEKARWALDRAGITYEEDGHLPLFHYLANIR
                     VGGKRTVPTLVTKDAGKKTVVRDSTDIIAWVDKQKPGSLIPLAGADEALAIEDDFDNH
                     FGPATRRWGYFYMLPNRDVDDVIGAGVPRWEKSLLKLARPFAVRYLKRSLKIDPDGVA
                     RSQKKIEETFSRVDEVLRDGRRFLTGDRFTVADLTFASLAAPILLPPEHPVQKFELGL
                     FPEEARVQIDRWRHTRAGQFGLRLYADERVRLPRAA"
     gene            complement(14202..15323)
                     /locus_tag="VMZ53_08650"
     CDS             complement(14202..15323)
                     /locus_tag="VMZ53_08650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28565.1"
                     /translation="MAFDGPLLGGRYRLGPRLGHGSQGEIFLARDEKATGAEAKEVVV
                     KRLRPRGTWKSFELFEREAKVLSQLKHPGVPRHLATLEEPPGTFNLVMQRAPGENLRD
                     FVKRRRLSETELRDILVRCLEILDYLHTRSPPIVHRDIKPSNIVRAPNTGHCSLVDFG
                     GVLDSIREKGGSTMVGTFGYMAPEQLHGQSVPATDIYALGATIVALAGGEEPEDVPRK
                     GLQMDLAKHLPSMDPSLRDALVEMTDPDPDKRPQRARDVVALLAKSKPRKKAARRNDK
                     ALVRTERAELAPRKMFSDVQEPLGTVLRLGVLGFGFGGWAGMLALRVSLTIAMWTAAI
                     IVFPTRKKVLGVGRELDSMLAEGQGGFTDMMKGAMAREP"
     gene            complement(15344..17239)
                     /locus_tag="VMZ53_08655"
     CDS             complement(15344..17239)
                     /locus_tag="VMZ53_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28566.1"
                     /translation="MSRWLWVVVLCVACEGKKAAPVAETPVASAPGVRAQPPFDVPAP
                     SDAIVAIGRGVSHACVVRASGAVDCWGQPIRKRCKGCFTDAAPDPKVRRVPGVTDAIA
                     IGADGRCVLRKTREIACLDETEVKFVAVPGIANATAVAGSQPPCAIVGSGDIVCASYE
                     KPGTVETVPNVHDATAIFVADFAMCALRKGGTVWCGMPGRKLDRIAGLDHVTEMAGDG
                     AIYQLRMCASIEKRGVHCYELLRYDTWHLSEGDKDDQPLLTTPIEGGTQIAVWREGPW
                     LQNYVAEVVAGGKVKRFELGRAVEDVPLVADAKLHARGCVVRAQGSVACWGQNDGGVL
                     AQPTTIGQVRLPPSPVVGLADVTSLALGDMHAKAMTRDGTIWEWGRADEDYKRHSVPH
                     RYEVIGGPGNFVEIAAVDGALCARHQNGETWCEQRNPYDHSVMTLAAPKDVVGLSWGS
                     DSDRHLVGDYLYCVRTRDRLATCGGDQIAGVQNVDEIAVGYDYACARHAGSISCWTVD
                     VVKRSHGQAFAIPGIAGATTLAGGPDQACAIHGKGKVTCWHVGEQGVEDVKQVLDGNA
                     MAIAIGAGTWSRLAFSRDNDAAPAAERLACAILADRTVTCWGANLYGELGDGSMIESA
                     QPIGIRL"
     gene            complement(17249..18337)
                     /locus_tag="VMZ53_08660"
     CDS             complement(17249..18337)
                     /locus_tag="VMZ53_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28567.1"
                     /translation="MRVAIAISAAVHVALFVWLEQAEPPAPVRPPAPSPVTIEVLDQV
                     TPPEPMTVALLDDHSVVTTPPDATPASHTPPAPGKHRRAGLAVSVSSTTGSTTGTTET
                     TAPAHSSLMTMRHPEKETLQGPTGSFVDRFLQNTKPLTDPDAKYGVISRHLRTGAWID
                     GATSDEVLAARMEQVAMNDARANAELKPNGTGTKTEHTTFKMKFNADGTVKEIKDKAN
                     WQQKSLFFAEFDVTDAMMRKQGIDPYSSYKKKVLDETREQRFEMGSKYRTQQLAQSRH
                     FMQRNLERLLASTTDKAQIKEGLFELWDDCAEAGSEELVAGGTAARQQLIGYIHAHLP
                     RDSADAFTSDEIAMLNKKRKSRSEFAPY"
     gene            18455..19087
                     /locus_tag="VMZ53_08665"
     CDS             18455..19087
                     /locus_tag="VMZ53_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS28568.1"
                     /translation="MIAALFARLFRQGPGGGDHLAAAQDGDRGAIARFYDAHVDGLYA
                     FVFYRVGRDATLAEDVVQETFTLALTKHADYDPARGSVGSWLTVLSRNVIRDHLKAHR
                     KADELQQTWERIDATLAQVFAAMADKPLPGEVLERAETRDLVHMAVANLPEQYRTALT
                     RKYVDGESLETLAKEMDISVDAAKSLLARARRAFRDTFATLSAHFSEAAQ"
     gene            19084..>20265
                     /locus_tag="VMZ53_08670"
     CDS             19084..>20265
                     /locus_tag="VMZ53_08670"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HUS28569.1"
                     /translation="MTRSTDSKDVLEQNVSTLLETGGEAPKLSDIAKARIKNELIAKH
                     ARDAVATKSRARTPLIAAGIGIAATAATAMIVAHFVGDPSAHVKGEVTKNGQLADGST
                     YIAEQGAKVTVLGPRRVRVEGAALLDVAPGKGTFVVETGHGNIEVLGTRFLVAADAAR
                     TTTAVVRGSVKLSNKDGNVTLHAGEQAVAEPGRPPTRGPAPRLSHLVSWAQQARRKAE
                     TDLEPLHHGTLYARDPGVRWNGTFGEQYPLPMKQLTVDVVVEDQVARVALDQTFHNNA
                     DEELEGVYRFAIPADAALQRLAMYVDGKLEESAVVERMQARRIYEELVYRRVDPALLE
                     WAGTGRLSLRVYPLPAHQDKRLMLAYTQSLPKLYSDWTLSVPLPEIDQPVGEVTFDVT
                     VR"
BASE COUNT         3322 a         6755 c         6712 g         3476 t
ORIGIN      
        1 cgtgctggcc gagaccgacg agcgtcgagc cggcagagac gccgtagccg tgcgcggagg
       61 ttgcgttgac ggtgtagcga ccgggatcga gacgcgcgat gcgaaacgcg cctttgtcgt
      121 cggtgacgtc cgtgttctga ccactgccac cgccgaagaa cgagaagctg ccccactcgc
      181 cgccttcggc ttcgacgaca acgccgggca cgggctcgcc cgactttgca tcgacgaccg
      241 tgcccgcaag cgatgactcg ggcgtgagca gcacttggaa cgcgcccggc gcttgcccgt
      301 tatcgctgcc cggggcgtag ccatcggcgg tcgcgttgac cgtgacatcg ccgggtttca
      361 cccacagcga gaacgcgccc gcctcgtcgg tctcggtcgg cggcatgaga tcgccgtcgc
      421 cccaatcgcg tgacgccgtc acgagcgcgt gtgcgatcgg gccaccgctg acatcggaga
      481 cgatgccggt gatctcgacg ccaccgctgc gcagcgcgat gtcgatgccg gtcttcgctt
      541 cgccggcctt cagctcgaac gttgtcggat cgtgcgggcc gctcgggtga tagctgccag
      601 ggcgaaacgt cttcgcggac gcggcgactt cgtactcggc ggcgaacagg ttcggcagcg
      661 tgtactgccc cttgtcgttc gcggtcacgc agatcggatc gcggacctcc tgcgcggaaa
      721 tctgctgcga gtagagctcg gcgcacacgc gcgcgcccgc gatcggtgcc ttctgctcgt
      781 cggtgatcgt gcccgcgatc gagccacgcg gctgcgtctt gggatcagga cgctcctgcg
      841 tcgacatggt tgcgccggac ccgaccgatt tctgcgccgc cttcgtcgaa gatttagaac
      901 gcgagctgtc gcgatcatga cagcgccaca acagcagcgt ggcgagcgcc gcgaggatca
      961 gagctgccgg cgcgactcgc gtcttcatca tgttcattgc acccttcgga accggcccgg
     1021 ttcccgattg ttgcctgaca ggcactgacc ttccccgttg cgcatcgtgc gcaacccact
     1081 tcagggcgcg agcacgcgat tgtcaatcag gcgcgtcgtg ccgacaaatg cggcgacagc
     1141 catgacggcg cgcttctcga cgcgcgggat ctccgagagc tcgtcggcgt cgcgaagctc
     1201 gagataatcg atgcgtacgc caggtgccgc ttccagctgc gcgcgtgccg tcccaacgag
     1261 cttcgccgca tcgcgttcgc cggctttgaa cgcagcctcg gcggccgcga gaccttttga
     1321 cagtgagagc gcagcctggc gctgctccac ggacaaatag gcattgcgcg acgacagcgc
     1381 gagaccgtcc gcttctcgga caatgggcac gccgacgatc tcgatgccga aatcgaggtc
     1441 gcgcaccatg cggcggatga tcgcgagctg ctggtagtcc ttctcgccga acactgcgag
     1501 gtgcggattc gtgatgtgga acagcttcgt cacgattgtc gcgacgcctg caaagtggcc
     1561 cgggcggctc gcgccacaca gcggcttctg cagctcgcgc acttcgacga aggtctgcgc
     1621 gccggccgga tacatcgcgc tcgcatcagg acaaaacgcc aagtcgatcc cacacgtcct
     1681 cgcctttgcg atgtcgccgg cttcgtcgcg cgggtaccga gagaggtcct cgttggggcc
     1741 gaactgtgtc gggttgacga agatcgtcag gatgacgatg tcggcgcgtt cacgcgcagc
     1801 gcgcagcagc gtcaggtggc cttcgtgcag cgcacccatc gtcggtacga ccgcaatgcg
     1861 gcgaccatca cgacgaagat cctcgacgcg cgcgcgcatc gccttggggt cgcggatgat
     1921 ctcgaaggga gggcgggcga acgtcacccg cgcagcctag cagtcggtca tcgcggtcga
     1981 gcagtcttga atcgtataaa ccgcggtcac gacgttcacc gaatcgtcgg tacaggaaaa
     2041 gttgtcgatc gcctgaaggc atttctgtgc ggctgcgcaa ctcggcggcg caacgggaag
     2101 ctggcgctga agcgtcgcga actggtcaca cacggtggcc atcgtcgggt ccgtgttgcc
     2161 gcagctcttg agcttcgtgc acatctttcc gaatgcgctg atcatgactg cgccaccgcc
     2221 ggctccgatc ggtgcaccgc cgctgaggcc gccgaacaga tcgttcgggt cattgggatc
     2281 gggcgtcatc ggatcaggcg cttgcggttg cggtgcgggc gctgcgggat taccgggctg
     2341 cgcccacgga tcgttgccct gtggcggcgt cagctggctc gacttcggat gcgtcgggtc
     2401 gacatccatc cgctcgggcg caggcggcgg cgcgccgatc ttcttgccgg ccttcttcgt
     2461 gtcctgcggc agcaccatga tcacgaccgc cgtcacgatc gcgagcaccg cgacaccgat
     2521 gacgagcatc ggccagctgc gccatagcga gcccgactgc gtttccttcg cgcgccagct
     2581 gccggtctcg cgcggcggca agatcggctg gctgccttcg cgcgcttgcg gaacggcgag
     2641 cgggattgcc ggtgcgtgcc cgttcgtgcc gttggtcgtc gtgatgatcg gcggcgcgaa
     2701 gttggtcgtc gtgctcgcgg gcgtgtgcag cgtgctcggc gcgggagccg agatcgcgtg
     2761 accattgagc gatggcagtg tggccgcggc ctgcggacga cccgcacgga acgacgtgag
     2821 gtcttgtttg cagtgcttgc accggcccgc caccgggtgg atgagtccac cgcagagggg
     2881 gcagggcatg cgcgcgtcac cgataccggg cgggcgggcg gtcgctgcca tcgtcatcaa
     2941 tgctgtcatc cttgccccct tgctgcaatg agatcgaacc cccgtcaggc cctgcctatt
     3001 ccgggtgatt gccaaggccg tcggcaggcc gcagagtgag cgccatggcg ccaccgcctc
     3061 cgcgggtttc ggagcacgag gtccgcatca agaacctcga tccccgccac gacggcgtcc
     3121 gaatcgcgca gctatcggac attcatgtcg gcaacatgac tcccgcatcg cacgtgcgcg
     3181 ccgccatcga cgccgcgaac cgagcgaagc ccgacctcgt gttcttgacc ggcgactacg
     3241 tgtgctggaa gcgcagcgag gcggagctcg tcgggcagca gctgcgcggt ctgcaggcca
     3301 agcgcgtgct ggctgtgctt ggaaaccacg actacttcac gtggggatcg ggcgtcgctc
     3361 gcgcgctcga gtcatgcggc tacgaagtgc tcaagaacca gacgaccatc tccgacgtca
     3421 acggcgcacc gctcgcgctc gtcggcgtcg atgatccggt cacgcgccac gacgacttcg
     3481 acgcggcgtt caaaggcgcg aacggctaca cgaagctcgt gctctgtcac gcgccggatt
     3541 cggggcctgc actcgcggag cgcggcgcgg acctcgtgct gtcgggccac acgcacggcg
     3601 gccagatcta cttcaagggc atcaccgatc ggctgatgcg ccgcatcggt ctcaactatc
     3661 gccgcggcat gttcacgttc ggcgacacga cgctctacgt gactccgggc gtcggcttct
     3721 cgggcattcc tcgtcgcatc ggcgaaggca ccgaggccga ggtcgccgtc ctcacgctgc
     3781 gcgcagcata gtcagtccgg atcgcgttcg cacacgtagc cgcgcgctgc cgagcaggcg
     3841 acgtcgttcc acttgccggt gaccccgtcg acagcaagac actcctcgtc gcgtgcctga
     3901 ccctcgccag cggcgaacgc ctcgtactcc catagctcgc catcggcgac ccacgtgaac
     3961 gccggcattc gctcggctcc gagccagaac tcgctcgcat agggcgcgag ctcgcgcacg
     4021 aagtcgcctt cacactccga caggatcgtc gcgaggtgcg caccgagtgc ctggcatcgg
     4081 gccttcgcca tcatccaggt cacgggctcg aagaagtgcc ggtaggagtg tccttgaaac
     4141 ttggtccagc ccggcgtctc gcagacgtga ctgcggtacc cagcgccgca gtcggtcggt
     4201 ccccagccgg ccgggtcctc ctgcgcgcag ccatcgccac cagacagcca cctggtgaac
     4261 gagacgggct cgctattggc ccacgtcgtc atgccgccca tctgtcgcgc gccaagccag
     4321 gtctggttac cgccgatgag gctcaccacg aacgagtcct cggcgctgct tccggttgtg
     4381 acgaggtagc cgccagccat tgcgcacgcg tcgacggcag gctgccacgg cgtgtacgcg
     4441 aggtagtcgt acacataaca gtgatcgccg aacatcatgc ccgtgcatgt ctcgtcgaac
     4501 gttgccgtgt tgttggtggg atcagtcgtg ttcgagcact cgatcggtga tcccttgcct
     4561 acgaatgtcg gctggtccac gcagccgacg agcacgagca cgagcacgcg cagcgctcgc
     4621 atcaccatac tccggacagc acgaatgctg tcgcctggtt ctgggtgctg acctcgatgg
     4681 tcgtcttggt gcccgagcgc aaccacaacc aggcggacac gcccgcgccg agcgcggaca
     4741 caccacccat cacccacgac gcgtgttgcc acgtgtgcgc acgatcgacg acggcgccgt
     4801 actcgctgcg tgtgatctcc gtcgacgccg cgtcgctcat gatgccgcgc gcgtggatat
     4861 aggcgccgag cgcggagaca cctaccgcag cagttgcgag cgtcgcgacg agcgccgggg
     4921 ttcgcgacct gtgctcggat cgcgtcgcaa tcggcgtggg ctccacgggc ttcggaggca
     4981 cgagctcgaa cacgacttga cgggtcgtgt gggtcagcgc ctcgacctca cggcgctgcg
     5041 gctcgagtcc tgtcgccgtg accacgatcg tcaccttgcc gaacggaacc catagcagtt
     5101 gggagccgac caacgtttcg tcgcgatcga gcacgtcgac gactgtcagc gatgcggtat
     5161 ccggcgtgac gacgatgtcg agcggcgtga agtcgccgga cttcagcgtc gcctcggtcg
     5221 cgtcgaggct cttgcgcacg agatcgagga acgaggcgtc catgctcttc ccgcgcctca
     5281 ggcactcggc gagatagatc tgcgctttcg gcaggacctt ggcgcgatgg tacgcaaccc
     5341 cgatgttgca cagatactcg gggcggggat cgagccgata agcggagcgg aacatcgtgg
     5401 caccctcgac gtgccgaccc gccttgacca gatcctcggc acgcttcgcg gcctcgattc
     5461 cagccaccgg atcgttctcg gcgtacgcaa ctccaccaag aagcgcgacc accacgaggc
     5521 ttctcacatc cacatcatat gacgggcaga tccgcgcggg tgggtctcac ctccaccaaa
     5581 cgacgtgcgc tttgcaccga cggcgctggc acgagatact cgaaccgatg gtcgatacgc
     5641 gcacctcgat gagcggcgga acgggcgccg cagacgaggc gtattgcgcg acttgtggca
     5701 cgtcgtacgc ggcgggaacc gtgcgctgcc cgcaggacgg tggcacgctg gtcaacttcc
     5761 ggtcgacgca ggatccgtgg atcggacgtg tcctcgatgg tcgctacgag atccgttccg
     5821 cgatcggggc cggcggaatg ggagcggttt atcgcgggaa gcagctgacg atggatcgtg
     5881 aggtcgccat caaggtgatc aagtcgaccg tcgtcgccga ccgcgaggtg gcgaagcgat
     5941 ttttgcgcga ggcgcgactg tcgagtcgac tggtgagcgg gaacatcgtg acggtgttcg
     6001 actccggcca gaccgacgat ggctcgctct atctcgtcat ggagctcatc gacggcaaga
     6061 cgctggcatc ggtgatcgcc gcgagcagtc cgatcacgag ccggcgcgtc gcgagcatcg
     6121 gtcggcagct ctgtgatgcg ctcgagaccg cccacgagct cggcatcgtg caccgcgacc
     6181 tcaagccaca caacgtcatg gtcctcagca cggcgctcgg gcgcgatcag atcaaggtcc
     6241 tcgacttcgg tctggccaag tcgatcacgg gtgattcgat gtcgcagatc accgcgacgc
     6301 acgcgatgct cggcacaccc gcttacatgg cgccggagca gatcgagaac ggcatgatcg
     6361 atggccgcac cgatctgtac gcggtgggtt gcatccttta cgagatgctc gccgggcggc
     6421 cgccgttcca ggccgcacag atcacggcgc tgttcgcaat gcatctcggc gaagcgcccc
     6481 cgccattgcc ggcggacgtt ccaccgacgc ttgcgcgcac ggtgatgcga ttgctcgaga
     6541 agagccctga cgcgcgattt gcgtctgccg ccgagctgcg cgccgtgctc gacgaagtca
     6601 gcccggcgac gccagtcacc ggagtgccgg tggtttccgc ggcagcgttg tcggccgcac
     6661 ggcctgttgc gccagcgcga cggcgcggtc tcgtgctcgg cagcgcgctt gcgctggtcg
     6721 ttgcacttgg cgtcggcgcg tttgttttcg tcacgcgaca cacgcctcct gccgccgagc
     6781 cgagcgcagt caagccgacg agcaatgtcg tggcgcctga accgccgcga acgccacctc
     6841 cacctccgat cgcagtgcct tcgcctgccg cagtgctggt gccggcgccg gtgcccgcgc
     6901 ccgtgacgac gacgccgaag acgacgaagg cgacaaaggc tacccggcca gcgaagcacg
     6961 cgaagccgaa gcctgacgcg ccgccgacaa cgaccacgaa acccgtgtca ccgcctgacg
     7021 agcttccgct ggcaccgatc agacgctgag ccgcgtcatc gctacatcac cgggacgtcg
     7081 gcgagcacga agccgtcacc ggcctcgatg ctgggcgcgc cgagctgaat gcccgcgagc
     7141 gacggcatcg gcagcttcga gagcgcgtcg tcggccttga cgccgatcac gccccacacg
     7201 ccggtgatga gcgcctcgac ttggttgtcg gtgagcggct cttcgacggt atcgccgttc
     7261 gcgagcacct gcgcgaagac cgtcggcgcg ccgaccgaga gcgtcacgtg accggcttgc
     7321 gtcggctgcg cttgcagcat cgtgcgcagc gagaccgcca tgtcatgcac gaccgcgccg
     7381 ttctcatcct tgacagtgac gaggaggtcg ccgacggaca tctcgagctc gccacctgcg
     7441 gtcgagaccg tcggcggaag cgcgagcttg acgtcgatcg tgcgagcgtc gtcgtcgagc
     7501 agtgccccga gcgcggggat cgagtcgatg ctgatcgact tgtcgaacgc gtctgcggcc
     7561 cagaggcccg cgaacagcgc gttgatgatg tcgtcgtcga gcgcgatgcc gaggttctgc
     7621 gtcgcgttca tgcccgtcac cgtggtgggc gtcgtgaggt acgtgccgcc ttcgccgccg
     7681 gtgaccttga gcttcgtgtc gaagccgacg aacagatccg tcgcgctgat cgcgactttc
     7741 gtcggtgcga tcgagatcgc ggcgtcgctg ccgaggatcg acgtgttgag aggcttcgcg
     7801 agcaaaccgg ccagcgcctt gtcggcgatc ggcgggacct tcgcttggat cgccttctcg
     7861 agcgcgctcg acacgccgtc gcgtgccttg cccttcagca ggctctcgat ttcgcccggc
     7921 acgccgccga cgtcgaccga gaagttgtcg aaggtcacgt tcgtgttcgc gagcgacgtc
     7981 atgatcttct cgctcgcaat gcgcgcgccg aggtcgccgt ggatcttcgt cttgctcgcg
     8041 cggaccgtga tcgtcgtcga gccgccgatg caggcgacct tgaacttcgc cgagagcttc
     8101 acgacgaggt tcgagagcac gacgtcggtg gcgaggaggt tcgtcttggg aatcagcgcc
     8161 gcatcgacat ccgtgaggct cacgctcgtg atgttgatcg tcgcgccgag gcagccgccg
     8221 ttgtcataga ccgggttcat cggctgcacg gccgcggtgt agtcgatggc cttcgcggtg
     8281 gtcgccacgg catttgcgac cgcggtgagc gccgtcgtgc ctgcgcgcgc gccgacctgg
     8341 ccggcttgcg tgccgtcgct cggagcgagc ttgcccgcga gcacggagcg aacgtcgcga
     8401 acgtcgttgc cgcccttgtc gatcgcgtgc gtctcgatga tcgcgatgcc cggaggaacg
     8461 gggaccgatg cggagaagct accgtccgcg gcaactgcga cttcggtgcc cgcgatcgtg
     8521 accttgacgc cggcgtggtc atccgtgaca tggccggtga cggtgacggt ctccgcgtcc
     8581 gcgacggtgc cgcgcgccgg gctcgaaacc tcgaggacgg gcggcttgtc agcctcttct
     8641 gacgaacatg cgaccacgga gaacaaaagc gcggctgcga ggaccctcat catgagaggt
     8701 caattcagca acggtcgtgc cgccggattg gccgcgtaac ccagcgaaag ctcagggcgc
     8761 aactccggta gccagggagg gaaaagaccc cctgaggaca gctgtccgca ggtcttcggc
     8821 gagctctttc tcgtaacgcg gtcggtcagc cggatcgacg cggaggatgg cggccggatg
     8881 gtgagtcgcg agcaacgttt cggcccagct cgtctggacg acgcgtccgc ggtcgcgcgt
     8941 gatcgagaag cgcgagccga tcagcgagag cgcggcggtg gcgccgagac aaacgatgac
     9001 gcgtggctgg atcgcagcga gctcggccgc gagccatggc ttgtacgcgc gaacttgatc
     9061 cgccgtcggt cgctgatgca gtcgcttctt gccacgcggg agaaagcgga agtgcttcac
     9121 cgcattcgtg acgtagagcg tgtcgcgagc gatgcccgca cgtgcgagcg ctgcatcgag
     9181 cactgcgccg gctggaccga cgaatgggcg gcccgctaga tcctcttgat cgccgggctg
     9241 ctcgccgacg agcacgatcc gcgcatcgac cgggccttcg ccaaagacgg tttgcgttgc
     9301 gggcccgcac agctcgcacg cactgcagtg tcgcgcttca tcgcgcagca ccggcagcgg
     9361 cttcccggtg acgtgcgtcg tcatcgtctc gacacgcgga gcggcggcgg cgagcagatc
     9421 ggaaatcagc gcggtctccg gcatcgcggc ccaatagcgc gcgggcatgt gctggcgcat
     9481 cacgcgcgga ttcgcgcgcg ccggattgaa gatcgacgcg tagtacgtgc gccagagatc
     9541 ctcgagctcg tcggcagcag gtgcggcatg gcgcggtacg ccggcaaaga actgcagctc
     9601 gttgccatcc cacgcgacgc tgcgctccgg tgtgaggatg gtccaccgca tcgcggcgaa
     9661 gcgctccgcg aagaacggcg ctgcacgttc gacgatttgg tgatcgggcg cgtaccacgc
     9721 gacgtagtga tcatccccgt gctccgagac gagccggaac cggacgaacg cgtgcatctt
     9781 gtggaggtcg cgcttcaccg cgccggccat cacgctgagc gcgcgaatgt ctggatccgc
     9841 ggcgtcctcg acgagcgcag gctcgccatg catgagccgc cacagcgcgc ggtagagcag
     9901 ggcgtcaggg cgttcgcgat gcaggatcgc gtcctcggcg agcgccacga agcgacgcgg
     9961 cacgcgcagc gtcgacggtg gtgcggtgag cggcgcttcg aacagcgacg gtgtgaacgt
    10021 cgcctcgtgc ggctgcacgt tattcgcgag cagcactcgc gcgcgatcgc gccactcggc
    10081 gtacgtcata gctgtcccgt gcggaaatcg aacgcgagct gctcgctctt cacgcgcttg
    10141 cgcaggtcga ggcgatcgat cagcaggctg tccgggttgt gatccgcggt gatgacgaac
    10201 ggcgatgcac gcttcatgac cacgcgcagc cgcgcgagat cttcgaggcg caccgttcgc
    10261 tgccggcgca tggcaacgat gcggtcgacg ttgcgcacgc cgaggccggg cacgcgcagc
    10321 agctcttcgc gatccgcggt gttcacgtcg atcggaaacc gcgcgcggtt gcgaagcgcc
    10381 cacgcgagct tcggatcgat ctcgagatcg aggttcggct gttcggtggt cgtcagctcg
    10441 tcgacggcga acccgtaatg gcgcaacagc cagtcggctt cgtagaggcg atgctcgcgg
    10501 acgagcggcg gtgcctgcag cggcagccgt gcatccgcgc gttgcgtcgg tgagtagccg
    10561 gtgtagtaga cgcgtcgcag cttgaactcg ctgtacagct gcgacgacgt cgcgaggatc
    10621 gtcgcgtccg gcgtctctgt ggcgccgatg atcatctgcg tcgattgccc ggcgggcgcg
    10681 aaccgcttcg gcgcatcggc gtgatgctcg gcaatcgtcg tcatcgactg ctggatcaca
    10741 ggcagccgct tctcgggcgc gagcttgtcg agatccgctg gcgtcggcag ctcgatgttg
    10801 acgctgagcc ggtcgcacca gcggcccgct tccgcgacca gcgcatcgga tgcgccggcg
    10861 accgccttga ggtggatgta gccgcggaac tcgtgcgtct cgcgcagcgt gcgagcgacg
    10921 cgggtcacga gctccatcgt gtagtccgga tcgcgcagca cgcccgacga cagaaacaac
    10981 ccttcgatgt agttgcggcg gtacatcccg agcgtcagct cgacgacctc ctccggcgtg
    11041 aactgcgcgc gcgcgatgtc actcgagcgg cgattcacgc agtacgtgca gtcgaacgtg
    11101 cacttgttcg tcagcaggat cttcagcagc gacacgcaac ggccgtcggg cgtgtagctg
    11161 tggcagatgc cattgcccgt cgaatgaccg aggcccgtct tcgtgcccga ccgcttcgca
    11221 ccgctcgatg cgcacgaggc gtcgtacttc gcagcatcgg cgaggatcgc gagcttctcg
    11281 cggacgttca tatgccggca cgcaacgtga agagatctcg atcatggcgc cgaagaaaaa
    11341 ctcgctcgtg aacaacatca atcgccgcaa gaaggcgggc aagagccgct cgaaggcgcg
    11401 ctcgacggtc tccgaggagt cgtacacgga gatgcgcaag ggctggacgg gttcgaagtc
    11461 cggccgccgc aagccgggtc gcaaggcgaa gaaatctcat cgctgaaacc cgagcactcc
    11521 gagcatgcga tcgaattcgt gcggcggaat gctgccgcct gtggcgcgcg ggtgaccgtt
    11581 cgcgaactca ccctcgacgc gtccgagcgg gaggtcgcgc aggaaaccga gcaggtcacg
    11641 cttgctcgca ctgcgcagcg cgaagttcac gcgacccggc agatagccgt cgttcgcgac
    11701 gagcacgacc ttcggcgcga ggcggccggc ccagcgcgtc gcgatgagcg gatggatctg
    11761 cgcgcccgac gtgaagcgaa tcagcgcgac gtcgccgacg accttcggtg ccacacgagc
    11821 aacgcgcgcg acctccgctt tgacctcctc gcgacacgcc tcgagcgcag cgactccgtc
    11881 gagctttctg cgcgcgatgt cctcgggccc gtgcgcggcg agcaagacct ccagcgcgag
    11941 ctcgggctga tagctcgctg cgcgacgcgc gctgttgagc agcgagatgg tctcgcgaac
    12001 atgcgtcttc ttcgtctgcg aggccggcac tgcgagctct tcggcgaatg gatggtgatc
    12061 gccgaggtca gcatgcgtcg cgaggagcgc gagccaaccg aggtcctgaa ggtcgacaat
    12121 ctcggagagc agctcgtacg cgaggaggcc tgtcggcgcc acgggctcgc accctgccgc
    12181 gctcacgaag atcgcgccat cgggtttttc acgagcgtcg tgatgatcga tgacgatcgt
    12241 cggcagatcc tcgacgatcg ggccggcccg actgcccatg tcgagcacga tcaatgcatc
    12301 ggcctgtaac cctgcgagcc gagctctcat cgaaggcgtg tgaacgtgct ctcccttgtt
    12361 tggcaatgcg atgaccggtt gaccgccgag gcgttcgatc gcgcgcatca ccagcacgcc
    12421 ggccgcgaga ccatcgacat ctcgatccgg cgcaacgatg atcttcgcgc gcttggcgca
    12481 gagctcgcgc agcaacgtct gggcttctcg catttgctcc gcaggcgcgg gccaggaatg
    12541 ggtggctgac atggttcgtc tcggcttgtg ctgcacgttt ctcgccgccc cgattcgatt
    12601 ccgcacgacc actgtgcgtt acgcgctttc actcgactcg cgggtacgcc gaggatttct
    12661 gaacgagctt gccttgcaca acgcggccgc tctcacgagt gcgatcgagt ggtgcatcga
    12721 ccacgacgtc ggagcgtttc gcgtgcagtc aggtcttctg ccgctctaca cgcaccccga
    12781 ggtcggctgg acgctcgacg gagaagaggg tgccggagtg agagctgcac tggctgccgc
    12841 cggacagcgc gcacgcgatg ccggagtccg gctgagcttc catcccgatc agttcgtcgt
    12901 gcccggctcc acatcgaaga acgtcgtgaa ggcgtcgctg cgagagctcg agtacatcgc
    12961 ggaggttggc gagctcatcg gcgccgatca aatgacgatt catggcggcg gcgcgcaggg
    13021 cggcaaaccg gaagcgctga agcgtctgct cgtcggtctc gccgacttga gcccgcgtgc
    13081 acgctcgctc gtcgcactcg agaacgacga tcgcacgtac cacgtcgagg acttgctgcc
    13141 gatctgcgag ggctccggca tcccgctcat ctatgacgtg caccatcatc gctgtaatcc
    13201 cgatagcctc agcgtgcagc gggcgaccga gctcgccgcg aagacgtggg gcctgcgcga
    13261 accgtgggtg cacctctcat cgccgctcgc agggtggcgc gacggtgatc cgcgtccgca
    13321 cgccgacttc atcaagtcga gcgacgtgcc agcgttctgg ctcgatcgca cgatgacgat
    13381 cgatgtcgaa gcgaaggcga aagagaaggc cgtgctgcgc ttgaagacgt ggatcgacgc
    13441 tcaagcggcg cgcggcaagc gaactcgctc gtcggcgtag agccgcaggc cgaactggcc
    13501 ggcgcgtgtg tgtcgccacc ggtcgatctg gacgcgcgct tcttccggaa acagccccag
    13561 ctcgaacttc tgcacgggat gctcgggcgg caggaggatc ggcgcggcga gcgacgcgaa
    13621 cgtcaggtcg gccaccgtga agcgatcgcc ggtgagaaac cggcggccgt cacgcaggac
    13681 ctcgtcgacg cgcgagaacg tctcttcgat cttcttctgc gagcgcgcga cgccgtctgg
    13741 atcgatcttg aggctgcgct tgaggtagcg aaccgcgaac ggacgcgcga gcttcagcaa
    13801 cgacttctcc cagcgcggca cgcctgcgcc gatcacgtcg tcgacgtcgc gattcggcag
    13861 catgtagaag tagccccatc gccgcgtcgc cggtccgaag tggttgtcga agtcatcttc
    13921 gatcgcgagc gcttcgtcgg cgccggcgag cgggatgagc gagcccggtt tctgcttgtc
    13981 gacccacgcg atgatgtcgg tcgagtcgcg gaccaccgtc ttcttgccag cgtctttcgt
    14041 gaccagcgtc ggcaccgtgc gcttgccacc gacgcggatg ttcgcgaggt agtggaacag
    14101 cggcaggtga ccgtcttcct cgtaagtgat gcctgcacgg tcgagtgccc agcgcgcttt
    14161 ctcgcagtag tgggagaacg gaatcgtgat cagcttgatc gtcatggctc cctcgccatc
    14221 gcgcccttca tcatatcggt gaatccgccc tggccctcgg cgagcatcga gtcgagctcg
    14281 cggccaacgc cgagcacttt cttgcgcgtc ggaaacacga tgatcgcggc cgtccacatc
    14341 gcgatcgtga gcgacacgcg aagcgcgagc atgccggccc acccgccgaa cccaaacccg
    14401 agcacgccga gacggagcac cgtgccgagt ggttcctgca cgtcgctgaa catcttgcgc
    14461 ggcgcgagct cggcgcgttc cgtgcgcacg agtgccttgt cgttgcggcg cgcggccttc
    14521 ttgcgcggct tgctcttcgc gaggagcgcg acgacgtcgc gtgcacgttg cggccgcttg
    14581 tctggatccg gatcggtcat ctcgacgagc gcgtcgcgca gcgacggatc catgctcggc
    14641 aaatgcttcg cgagatccat ctgcaggccc ttgcgcggaa cgtcctcggg ctcctcgccg
    14701 ccggcgagcg cgacgatcgt tgcgccgagc gcatagatgt cggtcgccgg caccgactgc
    14761 ccgtgcagct gctcgggcgc catgtagccg aacgtgccca ccatcgtcga gccgcccttc
    14821 tcgcggatcg agtcgaggac gccgccgaag tcgacgaggg agcagtgccc cgtgttcgga
    14881 gcgcgcacga tgttcgaggg cttgatgtcg cgatggacga tcggcggcga gcgcgtgtgc
    14941 aggtaatcga ggatctcgag acaacgcacg aggatgtcgc ggagctcggt ctcggacagc
    15001 cggcgccgct tcacgaagtc gcgcaggttc tcgcccggcg cgcgctgcat cacgaggttg
    15061 aacgtgccgg gcggctcttc gagcgtcgcg aggtgacgcg gcacgccggg gtgcttgagc
    15121 tgcgatagga ctttcgcctc gcgctcgaac agctcgaacg acttccaggt gccgcgcggc
    15181 ctgagccgct tgacgacgac ctctttggcc tctgcgcccg tcgccttttc gtcgcgcgcg
    15241 agaaagattt cgccctgcga tccatgaccg agccgcggcc cgaggcgata gcgccctccg
    15301 agcagcggac cgtcaaaagc catgcggtgt gcttagcacg cgatcacagg cgaatgccga
    15361 tcggttgcgc gctctcgatc atcgatccgt cgccgagctc gccgtacagg ttcgcgcccc
    15421 agcaggtgac cgtgcggtcg gcgaggatcg cgcaggcgag gcgctcggcg gcgggtgcag
    15481 catcgttgtc gcgcgagaac gcgagccgcg accaggtgcc agcgccgatc gcgatcgcca
    15541 tcgcgttgcc atcgagcacc tgcttcacgt cttcgacgcc ttgctcgccg acgtgccagc
    15601 aggtgacctt gcctttgccg tgaatcgcgc aggcctgatc cgggccaccg gcgagcgtcg
    15661 ttgcgccagc gatgcctgga atcgcgaacg cctggccgtg cgagcgcttc acaacgtcga
    15721 ccgtccagca cgagatgctg ccggcatgtc gcgcgcacgc gtagtcgtag ccgacggcga
    15781 tctcgtcgac gttctggacg cccgcaatct ggtcgccgcc acacgtcgca agacgatcgc
    15841 gcgtacgaac gcagtagagg tagtcgccga cgaggtgtcg atcgctgtcg cttccccacg
    15901 aaagtccgac aacgtccttc ggtgccgcga gcgtcatcac ggagtggtca taagggttgc
    15961 gctgctcgca ccacgtctcg ccgttctggt gccgcgcaca cagcgcacca tcgacggcgg
    16021 cgatctcgac gaagttcccc ggtccgccga tgacctcgta ccgatgagga accgagtgac
    16081 gcttgtagtc ttcgtccgcg cggccccatt cccaaatcgt gccgtcgcgt gtcatcgcct
    16141 tcgcgtgcat gtcgccgagt gcgagcgacg tgacatcggc gagaccgacg accggcgagg
    16201 gcggcagcct cacttggccg atcgtcgtcg gttgcgcgag cacgccgccg tcattttggc
    16261 cccagcaagc gaccgagcct tgcgcgcgta cgacgcagcc acgagcgtgc agctttgcgt
    16321 cggcgaccaa cggcacgtct tcgacggctc gcccgagctc gaaccgcttc accttgccgc
    16381 cggcgacgac ctccgcgacg tagttctgca gccatggccc ctcgcgccac acggcgatct
    16441 gcgttccgcc ttcgatcggc gtcgtcagta gtggctggtc gtctttgtcg ccctcactga
    16501 ggtgccaggt gtcgtaacgc agcagctcgt agcagtgcac ccctcgcttc tcgatcgatg
    16561 cgcacatccg cagctgatag atcgcaccat cacccgccat ctccgtcacg tgatcgagac
    16621 cggcgatgcg atcgagcttc ctgcccggca tgccgcacca cacggtgccg ccctttcgca
    16681 atgcgcacat ggcgaagtcg gcgacgaaga tcgcggtcgc atcgtggacg ttcggaacgg
    16741 tctcgaccgt cccaggcttc tcgtagctcg cgcacacgat gtctccgctg ccgacgatcg
    16801 cgcacggagg ttgcgaaccg gcgacggcgg ttgcgttcgc gatgccgggc acagcgacga
    16861 atttcacctc ggtctcgtcg agacatgcga tctcgcgcgt cttgcgcagc acgcagcggc
    16921 catctgcgcc gatcgcgatc gcatcggtca cgccgggaac gcgtcgcacc ttcggatccg
    16981 gcgcggcgtc ggtgaagcag cccttgcagc gcttgcgaat cggctggccc caacaatcga
    17041 cggcgccgct cgcgcgcacg acgcacgcgt ggctaacacc acgaccgatc gcgacgattg
    17101 catcgcttgg ggcaggcaca tcgaacggcg gctgcgcacg cacgcccggc gcactcgcca
    17161 ccggcgtctc ggccaccggc gcagctttct ttccttcaca cgcgacgcac agcacgacca
    17221 cccacaacca tcgcgacatt tcgcgagtct agtacggcgc gaactcgctg cgtgacttgc
    17281 gcttcttgtt gagcatcgcg atctcgtccg acgtgaatgc gtcggcgctg tctcgcggca
    17341 agtgcgcatg gatgtagccg atcagctgct ggcgcgccgc agtgccgccg gcgacgagct
    17401 cttcgctgcc ggcctccgcg cagtcatccc acagctcgaa cagcccctct ttgatctgtg
    17461 ccttgtcggt cgtcgatgcg agcaaccgct cgaggtttcg ctgcatgaaa tggcgcgact
    17521 gcgcgagctg ctgcgtgcgg tacttcgagc ccatctcgaa gcgttgctcg cgcgtctcgt
    17581 cgaggacctt cttcttgtag ctcgagtacg gatcgatgcc ttgcttgcgc atcatcgcgt
    17641 cggtgacgtc gaactcggcg aagaacaggc tcttctgctg ccagttcgcc ttgtccttga
    17701 tctctttcac cgtgccgtcg gcgttgaact tcatcttgaa cgtcgtgtgc tcggtcttcg
    17761 tgccggtgcc gttgggcttg agctcggcgt tcgcgcgtgc gtcgttcatc gcgacctgct
    17821 ccatgcgagc ggccaggacc tcgtccgacg tggcaccgtc gatccaggcc ccggtgcgga
    17881 ggtgacgcga gatgactcca tacttcgcgt cgggatcggt cagcggcttc gtgttttgca
    17941 gaaaccgatc gacgaagctg cccgtcggac cctgcagcgt ctccttttcg gggtgccgca
    18001 tcgtcatcag cgagctgtgt gccggcgcgg tggtctccgt cgtgccggtc gtcgagccgg
    18061 tcgtcgatga cacgctcact gcgagaccgg cccgccgatg tttgccgggc gccggaggag
    18121 tgtggctcgc gggcgtcgcg tcggggggcg tcgtcaccac actgtggtca tcgagcaacg
    18181 cgacggtcat cggctcgggc ggcgtcacct gatcgagtac ctcgatcgtg acgggtgagg
    18241 gcgcgggcgg tctaactggc gccggcggct cggcttgttc cagccatacg aagagcgcga
    18301 cgtgaacggc agccgaaatg gcgatcgcga cgcgcatacc ccttatgacc ccgaactagc
    18361 ggatatcgtt gcttcgctgg atttctcagg gtgattcgac acctcggcca cggccgatga
    18421 cacatgcaag cacgatgacc atttcggaga cgccatgatc gcagcgctct tcgcgcgctt
    18481 gttccgacaa ggaccaggcg gcggcgacca tctcgcggcg gcacaggacg gtgacagagg
    18541 ggcgatcgcg cggttctacg atgcccatgt cgacggtctg tacgccttcg tgttttaccg
    18601 cgtcggtcgc gacgcaacgc tcgcggagga cgtcgtgcag gagacgttca cgctcgcgct
    18661 cacgaagcac gcggattacg accccgcgcg cggttcggtc ggcagctggc tgacggtgct
    18721 ctcgcgcaac gtcattcgcg atcatctgaa ggcgcaccgc aaagccgacg agctgcagca
    18781 aacgtgggaa cgcatcgacg cgacgctcgc gcaggtgttc gccgcgatgg ccgacaagcc
    18841 actgcccggt gaagtgctcg agcgtgcgga gacgcgcgac ctcgtccaca tggccgtcgc
    18901 gaatcttccc gagcaatacc gcaccgcgct cacgcgcaag tacgtcgacg gcgagagtct
    18961 cgagacgctc gcgaaggaga tggatatctc cgtcgacgcc gccaaatctc ttctcgctcg
    19021 cgctcgccgc gcgttccgcg acacgttcgc gacgctgtcg gcgcacttct cggaggcagc
    19081 gcaatgactc gcagtaccga ttccaaagac gtcctcgaac agaacgtctc cacgctcctc
    19141 gagaccggcg gcgaagcgcc gaagctctcg gacatcgcga aggctcgcat caagaacgag
    19201 ctgatcgcca aacacgcgcg cgacgcggtg gcaacgaagt ctcgcgcccg cacgccgctg
    19261 atcgcggccg gtatcggcat cgctgcgacg gctgcaaccg cgatgatcgt cgcgcacttc
    19321 gtcggcgacc cgtcggcgca cgtgaagggc gaggtcacca agaacggcca gctcgccgat
    19381 ggctcgacgt atatcgccga gcaaggcgcg aaggtcaccg tgctcggacc gcggcgcgtc
    19441 cgcgtcgaag gtgcagcgct gctcgatgtc gcgcccggca agggcacgtt cgtcgtcgag
    19501 accgggcacg gcaatatcga agtgctaggc acgcggttcc tcgtcgctgc ggatgccgcg
    19561 cgcacgacaa ccgccgtcgt tcgcggctcg gtgaagctgt cgaacaaaga tggcaacgtc
    19621 acgctgcatg cgggcgagca agccgtcgcc gagccgggca ggccgccgac gcgcggaccc
    19681 gcgccgcgcc tctcgcatct cgtgagctgg gcgcagcaag cgcgccgcaa agcggagacg
    19741 gatctagagc cgctgcatca cggcacgctg tatgcgcgtg acccgggcgt gcgctggaat
    19801 ggcacgtttg gcgagcagta tccgctgccg atgaagcagc tgacggtcga tgtcgtcgtc
    19861 gaggatcagg tcgcgcgcgt cgcgctcgat cagacgttcc acaacaatgc cgacgaggag
    19921 ctcgagggcg tgtaccgctt cgcgatcccg gcagacgccg cgctccagcg cctcgcgatg
    19981 tacgtcgacg gcaagctcga ggagtcggcg gtcgtcgagc gcatgcaggc gcgccggatc
    20041 tacgaggagc tcgtctatcg ccgggtcgat ccggcgctgc tcgagtgggc cggcacgggc
    20101 cgcctctcgc tgcgcgtcta tccgctgccc gcgcaccagg acaagcgcct catgctcgcg
    20161 tacacgcaga gcctcccgaa gctctacagc gactggacgc tgagcgtccc gctccccgag
    20221 atcgatcagc cggtcggtga ggtcacgttc gacgtgaccg tccgt
//
LOCUS       DATLBZ010000164        11880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2390557, whole genome shotgun sequence.
ACCESSION   DATLBZ010000164 DATLBZ010000000
VERSION     DATLBZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11880
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390557"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VMZ53_08675"
     CDS             complement(<1..901)
                     /locus_tag="VMZ53_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HUS28570.1"
                     /translation="MVTQEGQGDEDFAALLAESFGQDSVKEGEILRGTVIAVGKDFAI
                     VDVGYKSEGQVPLEEFRMADGSIGVKPGDVVDVLLESRENDMGMVVLSKEKADRFKVW
                     DEISAACERDELIEGIITTRVKGGLSVTIRGGVKAFLPGSQVDLRPVRNLDAFLNQSF
                     KFKVIKFNKKRGNIVLSRRVLLEKERAALKESTLERLKEGQIVEGIVKNLTEYGAFID
                     LGGIDGLLHITDMSWGRVNHPSELFQVGDHVRVKVLKFNADTERVSLGLKQITEDPWS
                     RAAEKFVPGTVVRGKVVSLKDYGA"
     gene            complement(1036..1713)
                     /gene="cmk"
                     /locus_tag="VMZ53_08680"
     CDS             complement(1036..1713)
                     /gene="cmk"
                     /locus_tag="VMZ53_08680"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HUS28571.1"
                     /translation="MRRIVVTIDGPAGAGKSTVAKLLARRLGYRLLDTGAIYRAVALA
                     SQRQGIAWTDASACASIARDLDIRFDFVGDKNHVFLGDEDVSSAIRTPEVSQGASQVS
                     AHPPVRAALLDLQRRLGAGGGVVVEGRDTGTVVFPTAAAKFFLTATVEERARRRVAEL
                     TATGAEVNYEKTLAEIRERDDRDSSRDVAPMKPADDAVMVDSSAQTIEQVVENLAQTV
                     ETRASRS"
     gene            complement(1733..2737)
                     /locus_tag="VMZ53_08685"
     CDS             complement(1733..2737)
                     /locus_tag="VMZ53_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28572.1"
                     /translation="MHRVAIALALVAACGKKDDRAETGSAAPAVGSGASAVGSGSSAV
                     VAAVGDAATPDAAAAATGLGYELAAANIAPPAGSVAGTEPPTAESLHLELSYALAKWA
                     ANPRDKSLYAADFEGRDIADGRVDFVGYDEWMTKLPAKPAQTRNASIQSWLDPGTQVT
                     LGEAKVHVSYIYDDKDQYRQLMWRREGATWHLYKEQVNSQNSRPDDKVLALRNWLPTD
                     AKPLGKQLTVRFGIENMFAWIVVENGKGAQVVLDIWPEGTCTQVETPLPDPHFKEDVP
                     LGKLTCKDSGAGKDFVLVKSDDELGLRLYPAGKDAPMSTDHTIMIAKGAKVTLAPKTP
                     "
     gene            complement(2725..2961)
                     /locus_tag="VMZ53_08690"
     CDS             complement(2725..2961)
                     /locus_tag="VMZ53_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28573.1"
                     /translation="MAEQSIRELTATVKVLTKMLIDAGVVDEQVLRARIDASLDEEMH
                     ETNKIVECMSCQKRVPVSRAQHTAAGYVCDECTA"
     gene            3020..3802
                     /locus_tag="VMZ53_08695"
     CDS             3020..3802
                     /locus_tag="VMZ53_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28574.1"
                     /translation="MAEVSTERRWRTFADVVAFTLGTNVWITIVILPAVFVHALHGAP
                     MILASILPFAVLMLGLLRRSEIVLLGLFPSAILVPIALSPGIASSHVYGPIRFFLVAL
                     GVIAYLFGVSFFTTFHEPPAPKSVRQLSSAAQGPAVRWQRRERMYWMLSALALVIPTV
                     LIAWVNFDTTIAEDLGEMYPGRVALMTTALTAGAIVLWLAIYHYAFLGALRPHRTGDR
                     DLVGALAQARADAKAGKPRGRFYLSVGLALGAMLILILLRHL"
     gene            3811..3999
                     /locus_tag="VMZ53_08700"
     CDS             3811..3999
                     /locus_tag="VMZ53_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28575.1"
                     /translation="MRRYFFEILAVALIAGSLFFFKETLDYLARRDYVAALLVMLIGV
                     AVISVGKEMARLALVQRD"
     gene            4001..4894
                     /locus_tag="VMZ53_08705"
     CDS             4001..4894
                     /locus_tag="VMZ53_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28576.1"
                     /translation="MKRLLVTLLAACGGQATPPPAPSGPPAALQPTPAPDDVAVASVN
                     GKPVWGSCVAAQASRGITKQDALNECIDFELLAQAAEARGLALDPDVKLETRTALVSQ
                     LVAKDFEDKYQKPSDFGGLWDSLYQRNKTRFDHGEYRGTAYARINVAKNATPADDASA
                     KAIADEIAARLANERGLMPQHLVEIAEQVAGTRAKVAHEVVQPYSRHGLDPTYTDAMF
                     AIPEVGRTTPHAVRTPWGWDVILLNEIVPEEHMTAEQVAQKLLPEVKRTFFSTWTNQI
                     AQRIGAKIQVEQKNLPLLENL"
     gene            4891..5250
                     /locus_tag="VMZ53_08710"
     CDS             4891..5250
                     /locus_tag="VMZ53_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28577.1"
                     /translation="MTPFGHILQRAVQATPNAIGGAFADADGEMVDSYSTIDQTDWAI
                     LTAHYGIVMAHLNACFGTWHYGGPEYFIAEHMKVEVVCYAVDAGYFALLALTQPANLA
                     FALRQVRTAAAELRKEM"
     gene            5250..6587
                     /locus_tag="VMZ53_08715"
     CDS             5250..6587
                     /locus_tag="VMZ53_08715"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HUS28578.1"
                     /translation="MMRAAIIACVFALGLAASKVHAQPVDTTEFDPHSDSWDGMATWV
                     GLAEGRGYTVEVKTELDWDQLGANDILVLVYPLVRVEPSDLSAFVQVGGNVLIADEQG
                     ESKDALSSLGMLRVDFATPRASKYQDGRVWAPIATPRSSHEVAREIDEVVTNHPSILR
                     NVEGAETVVGFDDGAIVVAGERGTGRFVAVSDPSIFINRMMQYPGNVQLSVNILDWLD
                     RHQTPTRARNIVFVRGNTALYGDPRPFIDDPRGGQVARSITELNAWLWDRHWWFLTTG
                     AMKVLAGTLALLLLALAIAALPVRRGPKIDGAWLRFGRPSRRDEPYTLVGNADRAPSS
                     FLVLACMLRDQVQALLAEALGPGSTTEPLYTVGEGQLVEKLRKEKGDRAGAALSRVYR
                     RLRQLPSRGQAAAPWSSGHLARRDFDALYRDVAELCRTLGSPLVEADVDEAKT"
     gene            6587..7555
                     /locus_tag="VMZ53_08720"
     CDS             6587..7555
                     /locus_tag="VMZ53_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUS28579.1"
                     /translation="MQAALTHDKLSRVNQLASAIATEVGRAFIGSPTITEALLTALLA
                     RGHVLIEGYPGVAKTTLVKAFSSTLGCHYRRIQFTPDLLPSDITGTYILDMRTNQFVL
                     REGPVFTNVLLGDEINRAPAKTQSALLEAMQEKQVTIEGETRQLSEPFIVLATQNPIE
                     QEGTYPLPEAQVDRFLIKLKMGYPAIADEKRMLTAYDRTTPSVRAVVGPQEVLEMQAL
                     AQEVFVAEELIDYVLSLVAFTRNHARVYLGASPRAGLALMHASKGLALLRGRDFVLPD
                     DIRHLAPLVLGHRILMTPEAELEGQLGTVVVTEALDKVGYKMPKRQ"
     gene            7601..9274
                     /locus_tag="VMZ53_08725"
     CDS             7601..9274
                     /locus_tag="VMZ53_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUS28580.1"
                     /translation="MIPRLATRGKLVLGTALLFLLVGSLHGTPPLVALAGSILTVLLS
                     LYLMFYPTAILLRRKKIELSWWVPPGDQPGGALAVDRPFQLHLAFRNHGSRRLRILST
                     HVLGASGLALDERPSATVNRGQQVEVTTTVKPLCAGYHVLHGAALTFGDALGLFDIEA
                     FFPNPIAVKIFPRTLALRTQPVRAVGGALHEQVGLHHVRRRGLSGELRELREHTHGDP
                     FKFIAWKATARRRQLMVRDLENEIVTTHVVMLDVGAGMRQGPLGRAPIDWGCDATAAL
                     AKSAIGSSDRVGLVGFDTRPLVELGVDTGHHHYLQIIDRMLDVRSMVDADLTDVTAGE
                     LVALVARYLAHQEAIDVRIKIAPPLDDPRWTQIQAGPDGQLYDVAATGRLCKRLIDSM
                     VGKDPKDKKLKLPPVLEEDSSLRPLRQFCRLRGIELPYRSTWEHGKRAAGFHAAVERA
                     VASGRPDVVVIISDLSGFAEDEARMRRALARLRKAAGSVIALVPSATGFLPQATTSHG
                     RRVREIVVRDQRAALDPGRRLLIRHGIMVLEGSPLTSLDQLLHGRRVAA"
     gene            complement(9324..9488)
                     /locus_tag="VMZ53_08730"
     CDS             complement(9324..9488)
                     /locus_tag="VMZ53_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28581.1"
                     /translation="MRHGRDRWKDAIFIIAAVLLTAVSIGSVTSKAAGKPSEHKWKVT
                     VIESNLEIGR"
     gene            complement(9796..11136)
                     /locus_tag="VMZ53_08735"
     CDS             complement(9796..11136)
                     /locus_tag="VMZ53_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28582.1"
                     /translation="MAAPAKPDAETREHLLGVLSHFIARTGAAALLAEPVAPGERSFP
                     EPWAPNRAGVQLLLRRLAWHASVAAPGVGVDRAVGATSEREIVVEDKTAGAPPTERKP
                     ATRCELVEVSKERAVFALGFVGSDDVAGTLAHEIGMIYAVTHRPDEPDPYRTAEPPVI
                     SVDHDRDPELGSIATVYLGLGVLAANSAYQQYSRAGVFNGAYEPLEYDVLKAGALPMS
                     SLAYLLAVQAVVRGTDEAPKALKPPQRDEVEQWIDALEEEREELRARLGVSVADVETH
                     VNADGGAEGGAAQRRSRVERFADAEEAIVAEEPRSTRTAFRWHTHRGGVGMILGLVSG
                     TGLAATIGSAQAMGLLLLGGAVGHVVGRFVDVPRCSACATIVANDSTGCRKCGARLRG
                     DITSLSQRLEAEERLEAEQHRGSESGAESGAESGAGADSLGPYGAEPAENDERA"
     gene            11260..>11880
                     /locus_tag="VMZ53_08740"
     CDS             11260..>11880
                     /locus_tag="VMZ53_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28583.1"
                     /translation="MMPAGACVEYAARERAMTNLLDEVIVIPRRAYPRLPDLQVIERP
                     GWLQIITPSIRSGGLNEVIYSHLDAHDADATIAATIATYRELGLKFRWNAGPGSGPPD
                     LGERLERHGLAQSLGRGMARVIDDSVHARDDSQHTGDESSTRGRSRDASRVVVIEVDS
                     TTLDDYTHVMAAGWDGDAAALGVQHRHILADGRYRLFVAYCDGVPAA"
BASE COUNT         1984 a         4213 c         3609 g         2074 t
ORIGIN      
        1 aggcgccgta gtccttgagc gagacgacct tgccgcggac gaccgtgccg ggcacgaact
       61 tctcggcggc gcggctccac ggatcttcgg tgatctgctt gaggccgagc gagacgcgct
      121 cggtgtcggc gttgaacttg agcaccttca cgcggacgtg atcgccgacc tggaacagct
      181 cgctcgggtg gttgacccgg ccccagctca tgtcggtgat gtggagcagg ccgtcgatcc
      241 cgccgagatc gatgaaggca ccgtactcgg tcaggttctt gacgatgccc tcgacgatct
      301 ggccttcctt caggcgctcg agcgtcgact ccttgagggc ggcgcgctcc ttctcgagca
      361 gcacgcgacg cgacaacacg atgttgccgc gcttcttgtt gaacttgatg accttgaact
      421 tgaagctctg gttcaggaac gcgtcgaggt tgcgcacggg gcgcaggtcg acttgcgagc
      481 ccggaaggaa cgccttcacg ccgccgcgga tcgtgaccga gaggccaccc ttcacgcgcg
      541 tggtgatgat tccttcgatc agctcatcgc gttcacacgc ggcgctgatt tcgtcccaga
      601 ccttgaagcg gtccgctttc tctttcgaga gaacgaccat tcccatgtcg ttctcgcggg
      661 actccagaag cacgtcgacg acgtctccag gcttgactcc gatcgagccg tccgccatgc
      721 ggaactcctc gagcggaacc tgtccttcac tcttgtaacc gacatcgacg atcgcaaaat
      781 cttttccgac tgcgatcacg gtgccgcgga ggatttcacc ctccttcaca ctgtcttgac
      841 cgaatgactc ggcaagcagt gccgcaaagt cttcgtcgcc ttggccttcc tgagtaacca
      901 taaacaagca gatctcctac gactgccgag gttagatttg accgccgaga cggtccctag
      961 gcagggcgga cttggtacct ttcgcgaccc aaattgtcaa cgggaacggt gagatccgct
     1021 gcaacagcag tgtctctagc tccgtgacgc gcgggtctcg accgtctggg cgaggttttc
     1081 tacaacctgt tcgatggttt gcgcggagct atcgaccatg accgcatcgt ctgcgggctt
     1141 catcggggcg acgtcgcggc tggagtcccg gtcatcgcgc tcgcggatct cggccagggt
     1201 cttctcgtaa ttgacctcgg cgccggtcgc ggtcagctcc gccacgcggc gacgggcgcg
     1261 ctcttccacg gtcgccgtca gaaagaattt cgcagccgcc gtcgggaaca cgacggtccc
     1321 cgtgtcgcgg ccttcgacga caacaccgcc gccggcgccg agccgacgct gcagatcgag
     1381 gagcgccgcg cgcacgggag gatgcgccga aacctgcgat gcgccttggc tcacctcggg
     1441 tgtccggatc gcggacgaga cgtcctcgtc gccgaggaag acatggttct tgtcgccgac
     1501 gaaatcgaag cggatgtcga gatcgcgcgc gatgctggcg catgccgacg catccgtcca
     1561 cgcgatacct tggcgctgcg acgcgagcgc gaccgctcta tagatcgcgc cggtgtcgag
     1621 caggcgatag ccgagcctgc gcgcgagcag cttcgcgacg gtggacttcc ctgccccggc
     1681 gggaccatcg atcgtgacga cgatgcgacg catggcgcgc gtgtatcacg aatcagggcg
     1741 tcttcggcgc cagcgtgacc ttcgcgccct tcgcgatcat gatcgtgtgg tccgtggaca
     1801 tcggcgcgtc tttgcccgcg ggatagaggc gcaagccgag ctcgtcgtcg ctcttcacga
     1861 ggacgaagtc cttgccggcg ccgctgtcct tgcatgtcag cttgccgagc ggcacgtctt
     1921 ctttgaagtg aggatcgggc agcggcgtct cgacctgcgt gcacgttccc tccggccaga
     1981 tgtcgagcac gacctgcgcg cccttgccgt tctcgacgac gatccacgcg aacatgttct
     2041 cgatgccgaa ccgaacggtg agctgcttgc cgagcggctt ggcatcggtc ggcagccagt
     2101 tgcgcagcgc gagcaccttg tcgtcgggcc tgctgttctg cgaattgacc tgctctttat
     2161 agaggtgcca ggtcgcgcct tcgcgccgcc acatcagctg gcgatactga tctttgtcgt
     2221 catagatgta gctgacgtgg accttcgcct cgccgagcgt cacctgcgtg cccggatcga
     2281 gccacgactg gatcgacgca ttgcgcgtct gcgccggctt ggccgggagc ttggtcatcc
     2341 actcgtcgta tccgacgaag tcgacgcggc cgtcggcgat gtcgcggccc tcgaagtctg
     2401 cagcgtagag actcttgtcg cgcggattcg cggcccactt cgcgagcgcg tacgacagct
     2461 cgagatgaag cgactccgcc gtcggcggct cggtgccggc gacgctcccc gccggaggcg
     2521 cgatgttcgc ggcggccagc tcgtagccaa ggccggtcgc tgccgcggca gcatcaggag
     2581 tcgcggcatc gccgaccgca gcgacgactg ccgacgatcc agatccgact gccgacgctc
     2641 ccgagccaac ggcaggcgcg gcggatcccg tttcagcccg gtcgtccttc ttaccgcatg
     2701 cagcgacgag cgcgagcgcg atcgctacgc ggtgcattcg tcgcatacgt aaccagccgc
     2761 ggtgtgttgc gcgcgcgata ccggcacgcg cttctggcac gacatgcact ccacgatctt
     2821 gttcgtctcg tgcatctctt cgtcgagact cgcgtcgata cgggctctca gcacttgctc
     2881 gtcgacaaca cccgcgtcga tcagcatctt cgtcaggacc ttcacggtcg cggtcagctc
     2941 acggatggac tgctctgcca tgcgccgcat catgccgtaa ccacgggcgc gtcgcgattc
     3001 tgcgttgtac attcgagccg tggcggaggt ctccactgaa cgcagatggc ggacgttcgc
     3061 agacgtcgtc gcgttcacgc tcggaacgaa cgtgtggatc accatcgtga tcttgcctgc
     3121 ggtgttcgtt cacgcgctgc atggcgcgcc gatgatcctc gcgtcgatcc tgccgttcgc
     3181 ggtgctcatg ctcggcctgc ttcggcgctc ggagatcgtg ctcctcggtt tgttcccgtc
     3241 ggcgatcttg gttccgatcg cgctgtcgcc cggcatcgcg tcgtcgcacg tctatggccc
     3301 gatccggttc tttctcgtcg cactcggtgt gatcgcgtac ctgttcggcg tctcgttctt
     3361 cacgacgttt cacgagccac ccgcgccgaa gtcggtgcgt cagctgtcgt cggcggcgca
     3421 aggtcccgcc gttcgctggc agcggcgcga gcgcatgtac tggatgctca gcgcgttggc
     3481 gctcgtgatc ccgacggtgc tgatcgcgtg ggtcaacttc gacacgacga tcgcagagga
     3541 cctcggcgag atgtatcccg gtcgcgtcgc gctgatgacg acggctctca ccgcaggcgc
     3601 gatcgtgttg tggctcgcga tctatcacta cgcgtttttg ggcgccctcc gtccgcatcg
     3661 caccggcgat cgcgatctcg tcggggcgct cgcgcaggca cgcgccgatg ccaaggccgg
     3721 caaaccgcgc ggtcgcttct atctctcggt cggcctcgcg ctcggtgcga tgttgatcct
     3781 catcctgtta cgccatctgt gaggtgaacg atgcgccgct atttcttcga gatcctcgct
     3841 gtcgccctca tcgcagggag tctcttcttc ttcaaggaga cgctcgatta cctggcgcga
     3901 cgcgactacg tcgcagcgct gctcgtcatg ttgatcggcg tcgcggtgat ctcggtcggc
     3961 aaggagatgg cgcgcctcgc tctggtccag cgcgattgag atgaagcgtc tgctcgtcac
     4021 actgctggcc gcatgcggcg gtcaggcgac gccgccaccg gcaccgtcag gcccgccggc
     4081 tgcgctccag ccgacacctg cgcccgacga cgttgccgtc gcgagtgtca acggcaagcc
     4141 ggtgtggggc agctgtgttg cggcgcaagc atcacgcggc atcacgaagc aagacgctct
     4201 gaacgagtgc atcgatttcg agctgctcgc acaagccgcc gaggcgcgcg gcctcgctct
     4261 cgatccggac gtcaagctcg agacgcgcac cgcactcgtc agccagctcg tcgcgaaaga
     4321 cttcgaggac aagtaccaga agccatccga cttcggcggc ctgtgggact cgctgtatca
     4381 gcgaaacaag acgcgcttcg atcatggcga gtatcgcggc accgcatacg cgcgcatcaa
     4441 cgtcgcgaag aacgccacgc ccgccgacga cgccagcgcg aaagcgatcg ccgacgaaat
     4501 cgcagcgagg ctcgcgaacg agcgcgggct catgccgcaa cacctcgtcg agatcgccga
     4561 gcaagtcgcg gggacacgcg cgaaggtcgc gcacgaagtc gtgcagccgt attcgcgtca
     4621 cggcctcgac ccgacgtaca ccgacgcgat gttcgcgatc cccgaggtcg gtcgcacgac
     4681 accgcacgcc gtgcgcacgc cgtggggctg ggacgtcatt ctgctcaacg agatcgtgcc
     4741 cgaagagcac atgactgccg agcaggtcgc gcagaagctc ctccccgagg tgaagcgcac
     4801 gttcttctcg acgtggacga accagatcgc gcagcgcatt ggcgcgaaga tccaggtcga
     4861 gcagaagaac ctcccgctgt tggagaatct gtgacgccgt tcggccacat tctccagcgc
     4921 gccgtgcagg cgacgccgaa tgcgatcggt ggagcgttcg ccgatgccga cggagagatg
     4981 gtcgacagct actcgacgat cgatcagacc gactgggcga tcctcaccgc gcactacggc
     5041 atcgtgatgg cgcacctcaa tgcttgcttc ggaacgtggc actacggcgg ccccgagtac
     5101 ttcatcgccg agcacatgaa ggtcgaggtc gtgtgttacg ccgtcgacgc cggctacttc
     5161 gcgctgctcg cactcaccca accggcgaac ctcgcgttcg cactccgcca agtgcgaacc
     5221 gcagcggccg agctgcgcaa ggaaatgtga tgatgcgcgc cgcgatcatc gcttgtgtct
     5281 tcgcgctcgg ccttgcggcg agcaaggtgc acgcgcagcc cgtcgacacc acggagttcg
     5341 atcctcacag cgattcgtgg gacggcatgg cgacgtgggt cggcctcgcc gaaggtcgcg
     5401 gctacaccgt cgaggtcaag accgagctcg actgggatca gctcggcgcc aacgacatcc
     5461 tcgtgctcgt ctacccgctc gtgcgcgtcg agccgagtga cctatccgcg ttcgtgcaag
     5521 tcggtggcaa cgtgctcatc gccgacgagc agggcgagag caaagacgca ctcagctcgc
     5581 tcggcatgtt gcgcgtcgat ttcgccacgc cacgcgcatc gaagtatcaa gacggccgcg
     5641 tgtgggcgcc catcgcgaca ccgcgctcga gtcacgaagt cgcacgcgag atcgacgagg
     5701 tcgtcacgaa ccatccgtcg atcctgcgca acgtcgaggg cgccgagacc gtcgtcggct
     5761 tcgatgacgg cgctattgtc gtcgcgggcg agcgcggcac cggccggttc gtcgcggtca
     5821 gcgatccgag catcttcatc aatcggatga tgcagtaccc gggcaacgtg cagctgtccg
     5881 tcaacatcct cgactggctc gatcgtcatc agactccgac gcgggcccgc aacatcgtgt
     5941 tcgtccgcgg caacaccgcg ctctacggcg atccgcgccc gttcatcgac gatcctcgcg
     6001 gcggtcaagt cgcgcgctcg atcaccgagc tcaacgcatg gctctgggac cgccattggt
     6061 ggttcctcac gaccggagcg atgaaggtcc tcgccggcac gctcgcgctg ttgctgctcg
     6121 cactcgcgat tgccgccctg cccgtgcgtc gcggcccgaa gatcgacggc gcgtggctgc
     6181 ggttcggccg cccgtcacgc cgtgacgagc cgtacacgct cgtcggcaac gccgatcgcg
     6241 cgccgtcatc gttcctcgtg ctcgcgtgca tgctccgcga tcaagtccaa gcgctgctcg
     6301 ccgaagcgct cggccccggc tcgacgacag agccgctcta caccgtgggc gaaggtcagc
     6361 tcgtcgagaa gctgcgcaag gagaagggcg accgcgccgg cgcagcgctc tctcgcgtct
     6421 accgccgcct gcgccagctg ccaagtcgtg gccaagccgc agccccctgg agcagcggcc
     6481 acctcgcacg ccgcgatttc gacgcgctgt atcgtgacgt cgccgagctg tgtcgtactc
     6541 tcggctcccc tctggtagaa gcggacgtgg acgaagcgaa gacctgatgc aagccgccct
     6601 cacacacgac aagctcagcc gcgtcaatca gctcgcgagt gcgatcgcta ccgaagtcgg
     6661 tcgcgctttc atcggctcgc caacgatcac cgaggcgctg ctcaccgcgc tactcgcccg
     6721 cggccacgtc ctcatcgagg gctacccagg cgtcgcgaag accacactcg tcaaggcgtt
     6781 cagctctacg ctcggctgcc actaccgccg cattcagttc acgcccgacc tgctgccgtc
     6841 cgacatcacg ggcacgtaca tcctcgacat gcgcacgaat cagttcgtgc tgcgcgaggg
     6901 cccggtcttc acgaacgtcc tcctcggcga cgagatcaac cgcgcgcctg ccaagacgca
     6961 gtccgcgctg ctcgaagcga tgcaggagaa gcaggtcacg atcgaaggcg agacgcgtca
     7021 gctctccgag ccgttcatcg tgctcgcgac gcaaaacccg atcgagcaag aaggcaccta
     7081 cccgctgcca gaagcgcagg tcgatcgatt cctcatcaag ctgaagatgg gctacccggc
     7141 gatcgccgac gagaagcgca tgctgaccgc gtacgaccgc acgacgccga gcgttcgcgc
     7201 agtcgtcggc ccgcaagaag tcctcgagat gcaggcgctc gcccaagagg tgttcgtcgc
     7261 cgaagagctg atcgactacg tgctcagcct cgtcgcattc acgcgcaatc atgcgcgcgt
     7321 ctacctcggc gcatcgccgc gcgcgggtct cgcgctcatg catgcatcga agggcctcgc
     7381 gctccttcgc ggccgcgact tcgtgctgcc cgacgacatt cgccacctcg cgcccctcgt
     7441 cctcggccac cgcattctga tgacgccgga agccgagctc gaaggccagt tgggcaccgt
     7501 cgtcgtcacc gaagcgctcg acaaggtcgg ctacaagatg ccgaagaggc agtagcgcga
     7561 cgatgagaac gacgacgacg gcgacgcgga gaccagacgc gtgattcctc gtctcgcgac
     7621 ccgcggcaag ctcgtcctcg gcaccgcgct actcttcctg ctcgtcggct cgctgcacgg
     7681 cacgccgccg ctcgtcgcgc tcgcgggctc gatcctcacc gtcctgctct cgctctactt
     7741 gatgttctat ccgacggcga ttctgctccg tcgcaagaag atcgagctgt cgtggtgggt
     7801 tccgcccggc gatcaaccag gcggcgcgct cgcggtcgat cgcccgttcc agctccacct
     7861 cgcattccgc aatcacggca gccgccgcct tcgcattctc tcgacgcacg tgctcggtgc
     7921 atcgggcctc gccctcgacg agcgtccgtc ggccacggtc aatcgcgggc agcaagtcga
     7981 agtcaccacc accgtgaagc cgctctgcgc cggctatcac gtgctgcacg gcgcggccct
     8041 gacattcggc gacgcgctcg gcctgttcga catcgaggcg ttcttcccga atccgatcgc
     8101 ggtcaagatc ttcccgcgca cgctcgcgct ccgcacgcaa ccggtccgcg cggttggcgg
     8161 cgcactgcac gagcaagtgg gccttcacca cgtgcgtcgt cgcggcctct ccggcgagct
     8221 gcgcgagctg cgcgagcaca cgcacggcga tccgttcaag ttcatcgcct ggaaggcgac
     8281 cgctcgccgg cgccagctga tggtccgcga cctcgagaac gagatcgtca ccacgcacgt
     8341 cgtcatgctc gacgtcggcg cgggcatgcg ccaaggcccg ctcggacgcg caccgatcga
     8401 ttggggctgc gacgcgaccg ccgcgctcgc gaaatccgcg atcggctcga gcgatcgcgt
     8461 aggcctcgtc gggttcgaca cgcggcccct cgtcgagctc ggcgtcgaca ccggccacca
     8521 ccactacctc cagatcatcg atcgcatgct cgacgtgcgc tcgatggtcg atgccgacct
     8581 taccgacgtg acggccggcg agctcgtcgc cctcgtcgcg cgctacctcg cgcaccaaga
     8641 agcgatcgac gttcgcatca agatcgcgcc gccgctcgac gatccacgct ggacccaaat
     8701 ccaagcgggc cccgatggcc agctctacga cgtcgccgca acgggccgtc tctgcaagcg
     8761 cctcatcgac tcgatggtcg gcaaagatcc gaaagacaaa aagctgaagc taccgcccgt
     8821 cctcgaagag gactcctcgc tgcgcccact tcgccagttc tgccgcctgc gcggcatcga
     8881 gctgccctac cgctccacgt gggagcacgg caaacgcgcc gccggctttc acgcagccgt
     8941 cgaacgcgcc gtcgcatcag gtcgtcccga tgtcgtcgtc atcatcagcg acctctccgg
     9001 cttcgctgaa gacgaagcac gcatgcgtcg cgcgctcgct cgccttcgca aagccgccgg
     9061 ctccgtcatc gcgctcgttc cctcggccac cggcttcttg ccgcaagcca cgacgtctca
     9121 cggccgccgc gttcgcgaga tcgtcgtgcg cgatcagcga gccgcactcg atccaggccg
     9181 gcggcttctc atccgccacg gcatcatggt gctcgagggc tcgccgctca ccagcttgga
     9241 tcagctgcta cacgggcggc gtgtcgctgc gtaaccgcag cgaccagctt caaatcaata
     9301 gacaggtaca gacagcgacg ggactagcga ccgatttcga gattgctctc gatgacggtg
     9361 accttccact tgtgctccga gggcttgccc gcggccttgg aagtcaccga gccgatcgac
     9421 accgcggtca gaagcaccgc agcgatgatg aagatcgcgt ctttccagcg atcccgtccg
     9481 tgcctcacct ggatttcgtt caagttgctc attgtttcca ttcctccggg acgcgaacga
     9541 actcccatat tcaccttaac gattcggacc tggggagtca cgaaatacgt ccgaaatttt
     9601 ggtcagggag atcgaatttc tcgacgagaa ctacgcagat gtgccgtcgg cgcacacgac
     9661 atcccacacg cgacactctg cgacagacac gcgacatacg cgctcccgcc aatcactctc
     9721 gcaatcatga cgcgaccgct cacacgatgc gttttgcgtt ctcgatatga aggacgcggg
     9781 aggcgccccg tcggtttacg cgcgctcgtc gttttcggcc ggctcggcgc cgtacggacc
     9841 tagcgaatct gcgcctgcgc cggactctgc cccggactct gcgccggact ctgagccgcg
     9901 atgctgctcg gcctcgagtc gctcctccgc ttccagtcgc tgcgacaagc tcgtgatgtc
     9961 accgcgcaga cgcgcgccgc acttcctgca gccggtcgag tcgttcgcga cgatcgtggc
    10021 gcatgccgaa cagcgcggca catccacgaa gcgccccaca acatgcccca ccgctccgcc
    10081 gagcagcagc aggcccatcg cctgcgccga tccgatcgtc gccgctaacc ccgttcccga
    10141 cacgagtccc aaaatcatcc ccacgccgcc gcggtgcgta tgccaccgaa acgcggtccg
    10201 cgtactgcgc ggctcctccg cgacgatcgc ctcctctgca tcggcaaacc gctcgacgcg
    10261 acttctccgc tgcgccgcgc cgccctccgc gccgccgtcc gcgttcacat gcgtctcgac
    10321 atccgctacc gaaacaccaa gccgcgcgcg cagctcctcg cgctcctctt cgagcgcatc
    10381 gatccactgc tcgacctcgt cgcgctgcgg aggcttcaac gcctttggcg cctcatccgt
    10441 cccgcgcacg accgcctgca ccgcgagcaa gtacgcgagc gatgacatcg gcagcgcacc
    10501 agccttcaag acgtcgtact ccagcggctc gtacgcgcca ttgaacacgc ccgcgcggct
    10561 gtactgctga tacgccgagt tcgccgccaa cacgccaagc cccagataca cagtcgcgat
    10621 cgatccgagc tcgggatcgc gatcatgatc gacgctaatc accggcggct ccgcggtccg
    10681 atagggatcc ggctcatccg gccggtgcgt gaccgcataa atcatcccga tctcgtgcgc
    10741 caacgtccca gccacgtcat cgctaccgac gaaccccagc gcaaagaccg ccctctcctt
    10801 cgagacctcg acgagctcgc atcgcgtcgc aggcttcctc tcggtcggcg gcgcgccggc
    10861 cgtcttgtcc tcgacgacga tctcgcgctc gctcgtcgcc cccaccgccc gatccacacc
    10921 aacgccgggc gcagccacgc tcgcatgcca cgccagtcgc cgcagcaaca gctgcacgcc
    10981 ggcgcgattc ggcgcccacg gctccggaaa gctccgctcg cccggcgcca ccggctccgc
    11041 aagcaacgcc gccgccccgg tacgcgcaat gaagtgcgag agcacgccga gcaaatgctc
    11101 gcgcgtctcc gcgtcgggct tcgcaggcgc cgccatcgcg cgactatacc gtcacgcggt
    11161 gcgctacaca gccgccgatg ccgcggccca gtcgcgcgcg cctcggcgcg gtcccacggg
    11221 acgcgagcgc atgacaatcg gcggagtgtc ccaaagaacg tgatgcccgc cggcgcgtgc
    11281 gtcgagtacg ctgcgcgcga gcgcgcgatg acgaatcttc tcgacgaggt gatcgtaatc
    11341 ccgcgccgcg cgtacccacg attgccagat ctccaggtga tcgagcggcc gggctggcta
    11401 cagatcatca cgccgtcgat tcgaagtggc ggcctgaacg aggtcatcta ctcgcacctc
    11461 gacgctcacg atgccgacgc gacgatcgcc gcgacgatcg cgacgtatcg cgagctcggt
    11521 ctcaagttcc gctggaatgc ggggccaggt agcggcccgc ccgatctcgg cgaacgcctc
    11581 gagcggcatg gcctggcgca aagtctcggg cgcggaatgg cccgcgtgat cgacgactcc
    11641 gtccacgctc gcgatgattc gcagcacacg ggtgacgagt cgtccacacg cgggcgttcg
    11701 cgcgatgcga gccgcgtcgt cgtcatcgag gtcgactcga ccaccctcga cgattacacg
    11761 cacgtcatgg cggccgggtg ggacggcgac gccgccgccc tcggcgtgca gcaccgccac
    11821 atcctcgcag acggtcgcta tcgcctcttc gtcgcgtact gcgatggcgt gccggccgcc
//
LOCUS       DATLBZ010000165         6545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5373345, whole genome shotgun sequence.
ACCESSION   DATLBZ010000165 DATLBZ010000000
VERSION     DATLBZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5373345"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1920)
                     /locus_tag="VMZ53_08745"
     CDS             complement(<1..1920)
                     /locus_tag="VMZ53_08745"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS28584.1"
                     /translation="MKSLTRWRLCCGVLSAVLAYSWIGGSDAEQPTTSQTRRNTRGGM
                     QVHVDAKAAGISLDELVEKLLAAKTVEEIEPLAKTLGTVGDDKTIDAVESLVADTRAG
                     VPAAIVTAYGEIGSDHAVDVLREHSHDARSEVRTAVIEALGSSHNPRAEVTIISAARD
                     PDVTIAGSGIWALGELGTDGALAVLEDIAAAGVEETSSSAIVAIARIDTPASHEAISR
                     LIDSPSVEVARRALASVTVVDDALMLKINTIVKTGETGLIEAALEALSHAGSAAVPTL
                     VEVSKNGTPNTRLAAISALGRIESPDAMQALRDLLEDEDINVVRTVIAALSAMSTDEA
                     RDLLISSALSDRDDVSTRAVEALLEMHGPEIEQALVEIVRAQPRAHEDILRHLVIAGN
                     KEGIALVMTLAHSSDTEERLMAIRIFASAGTSDALVLALELVRAEHGDGKAQALRMLA
                     QANPGDPAVFELLRDTLRTGTPDEAVAALAVLARAGTEDAREAIVAAVSSSDADICGA
                     AVAAMANFRMTPEAANALRGAAEAHPELASSVMRQLFTVNSPVAVQIAERLLRSSDRG
                     ESEMALRALQEAGTAEAGELLVRTARTGDPDLRVSALRALAGSGDKRGVEVMIGALRD
                     GNAAVRETAIYGLGSL"
     gene            2255..3037
                     /locus_tag="VMZ53_08750"
     CDS             2255..3037
                     /locus_tag="VMZ53_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS28585.1"
                     /translation="MAPAAKVAEHQGTTVHRVLLVDDHEMYLKYCTRDFVRAGIAVSS
                     TQTLAGAVEIVRREQPDVVVVDLFLTPPESGLEVIRAVKEIDPNVFCILVSAHMSVSH
                     ALMGVRAGADDVFIKPVAARQVISRVEQGVVDAPNGATPTLHEVEWEHISRVLHDHKG
                     NISHAAESLGIFRQSLQRKIQKYAPRVIATGEEQPYPVAPPKLDEEEQRAPAPAAKKP
                     KAKAPAKQSARASATPPSHSESPSKSGGKSKSRASAKSRRRH"
     gene            3069..3932
                     /locus_tag="VMZ53_08755"
     CDS             3069..3932
                     /locus_tag="VMZ53_08755"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS28586.1"
                     /translation="MERLADRVAWITGGASGIGLAIAHRLADEKVKLVLVDIEQAALD
                     AAEAALKAKGASVLAIRADVASSAEVAAAAARAKETFGLVHIIVNNAGVGGNGGPMWS
                     IAEGDWQWALGVNLWGVIHGIRHLLPALIESGEEGHVVNTASMAGLTSTPFMAPYTAT
                     KHAVVAMSECLAKELELAKSKVKVSVLCPGFVQTNINTSDRNRSKDYGSKTQSAGADK
                     FRDVLATLVSSGKPAATVGEQVVSAIKTDKFYILTHPEMKPAIEHRMKQILEEQPPGI
                     DPMMRALFGSQ"
     gene            complement(3933..5153)
                     /locus_tag="VMZ53_08760"
     CDS             complement(3933..5153)
                     /locus_tag="VMZ53_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28587.1"
                     /translation="MRYLLAATLLMCGCGSHAHGTGDDVDASLPPDASTDFDNDGLSN
                     TEEGCEAPPGRDTDMDGVPDCKDTDSDDDGYDDALEGLADWDKDGVPNYIDGFNDGPP
                     PTLTFVAISTPFNTPIGIDYHEPTHTVILSVNYGTGGQPHNFARIQADGMELPFSDYA
                     GMVEEVKIATARSGKDNPAGWDVGTMFTGNGIDGQIVKISPDGSVITNPWIDLPGTNN
                     GLMRGSLYVDRTGIFDGDLIVATTTGEVWRVNKAGTPTPITVPKGVHLEGLIVVPNVP
                     KRYGPIAGKIIAGAEGEGLMWIISPNGDATTINLGVAIEDIDIVMPGENFFGVNFGTS
                     RLLGVTAKECEPMIGDILLTQENVVAGTSGLFRLQWNGSALAAVAVPVAAGSATVGQW
                     EHTTFANASIVEIQ"
     gene            5207..6301
                     /locus_tag="VMZ53_08765"
     CDS             5207..6301
                     /locus_tag="VMZ53_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28588.1"
                     /translation="MYLLVAVTIVRAVVVGRVDGGTWTDAPTEARSEQKAELAAVVLA
                     RDGKRDVVLVPEGVTDLRLGGRKIAAKSLRPLTPKTIRWSTVEPHGFRAHDATNGATS
                     DFYSNVSTESKTFGTWLGYDHVDYFEHVMIENGAAIIPATIVPSDTTAVQIAGLGTLR
                     FKVEVELADGTHAASPGADATDSFGILPSVHRVSIRSADGFLGWLSAYLLVPEVFGSA
                     GPGKNHQTERFIGADCADVMIGAMRRDGHKLPYTNVAGLPSVTKTVANAAELDETGNP
                     STEIAGVKPGDLIRIDYGGSLRHHTPRAFDHVAALWEDKSDPKGPLEGAADGRLDGFD
                     LVIHVGHPRLVIEPLARQAPATIDVLRWKN"
     gene            complement(6353..>6545)
                     /locus_tag="VMZ53_08770"
     CDS             complement(6353..>6545)
                     /locus_tag="VMZ53_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28589.1"
                     /translation="LYEQIADQNSKLSLHLVGSSRAADTNNDYKLDKLVFGTWQGTMT
                     YEADDATLAQPADYDGTRM"
BASE COUNT         1129 a         2249 c         2052 g         1115 t
ORIGIN      
        1 cagcgagccg aggccgtaga tcgcggtctc gcgaaccgcg gcgttgccat cgcggagcgc
       61 gccgatcatg acctcgacac cgcgcttgtc tccggagccc gcgagcgcac gcagcgcgct
      121 gacacgaagg tcgggatcgc ccgtgcgtgc cgtgcgaacg agcagctcgc ccgcttctgc
      181 ggtgccggcc tcttgcagcg cacgcagcgc catctcgctc tcgccgcgat cgctgctgcg
      241 gagcaggcgc tctgcaatct gcaccgcgac cggtgaattg acggtgaaga gctgccgcat
      301 caccgacgac gcgagctcgg ggtgcgcttc cgccgcgcca cgcaaggcgt ttgctgcttc
      361 gggcgtcatc cggaaattcg ccatcgcggc gacggcagcg ccgcagatat cggcatcgct
      421 gctcgagacc gcggctacga tcgcctcgcg cgcatcttcg gtgcccgcac gagctaacac
      481 cgcgagcgca gcgaccgcct cgtcgggcgt acccgtgcgc agcgtgtcgc gcaggagctc
      541 gaagacagca gggtcgccgg gatttgcttg cgcgagcatg cgcagcgcct gcgctttgcc
      601 gtcgccatgc tccgcacgca cgagctcgag cgcgagcacg agtgcatcgc tcgttcccgc
      661 cgacgcgaag atgcggatcg ccatcaggcg ctcttcggta tcggagctgt gcgcgagcgt
      721 catcacgagc gcgatgccct ctttgttacc tgcgatcacg agatgacgaa ggatgtcttc
      781 atgtgcgcgc ggctgcgctc tcacgatctc gacgagcgct tgctcgatct ccggaccgtg
      841 catctcgagc aacgcctcga cggcgcgagt cgacacgtca tcgcggtccg acaacgcgct
      901 ggagatcagc aggtcgcgcg cctcgtccgt cgacatcgcc gacagcgcag cgatcacggt
      961 gcgcacgacg ttgatgtcct cgtcctcgag cagatcgcgc agcgcctgca tcgcatcggg
     1021 gctctcgatt cgaccaagcg cgctgatcgc agcgagccgc gtattcggcg tgccgttctt
     1081 cgacacttcg acgagcgtcg gcacggccgc gcttcccgca tggctcagcg cctcgagtgc
     1141 cgcctcgatg agcccggtct cgccggtctt cacgatcgtg ttgatcttca gcatcagcgc
     1201 gtcatcgacg acggtcaccg atgcgagcgc gcgacgcgca acctcgacgc tcggcgaatc
     1261 gatcagacgc gagatggctt cgtgagacgc aggcgtgtcg atccgcgcga tcgcgacgat
     1321 ggcactgctc gatgtctctt cgacaccggc cgccgcaatg tcctcgagga ccgcgagcgc
     1381 accgtcggtg ccgagctcgc cgagcgccca gatgcccgac cccgcgatcg ttacatcggg
     1441 atcgcgtgcg gcactgatga tcgtgacctc ggcgcgcgga ttgtgcgagc tgccgagcgc
     1501 ttcgatcacg gccgtgcgga cttcgctgcg tgcgtcgtgc gagtgctcgc gcagcacgtc
     1561 gacggcgtga tcgctgccaa tctcgccata cgccgtgacg atcgctgccg gtacgccagc
     1621 cctcgtgtca gcaacgagcg actcgaccgc gtcgatcgtc ttgtcatcgc caaccgtgcc
     1681 gagcgtcttc gcgagcggct cgatctcttc gaccgtcttc gcggcgagca gcttctcgac
     1741 aagctcgtcg agcgagatgc cggccgcctt tgcatcgacg tgcacctgca tgccgccgcg
     1801 cgtgttgcga cgcgtctggc tcgtcgtcgg ttgctccgcg tcggagccac cgatccacga
     1861 gtacgcgagc acggcgctca gcacaccgca gcagagtctc cagcgagtca gtgacttcat
     1921 cgccctgctc caaatgacac tacgcaccgc atttcggttt cattcgattg ctgtggcgca
     1981 atgccacggt ttggtttcac ccattcgctg cgagcggttg ccacgatctg gcaacgcacc
     2041 ggaagcagac tggaactatg aatgtcggcg agatcctcgt ccgcatcgga gtgatcggag
     2101 aggcacacac ggtgctgcgt gatggcctcg acgcacgtcg cacgttcgac ggcgagcggc
     2161 ctcgccgtgc tcctactccc gcccgatcgt ttgggcccca aagtaaaaat gagatcgctt
     2221 gacgaggcgc gcagtcgcct tctaccgttc ccaggtggca cctgcggcca aggttgctga
     2281 gcaccagggg acgacggttc accgcgtcct gctcgtggac gaccacgaga tgtatctcaa
     2341 gtactgcacg cgagatttcg tccgtgcggg gatcgcggtc tcgagcacgc agacgctcgc
     2401 cggtgctgtc gagatcgtgc gcagggagca gccggatgtc gtcgtggtcg acctgttcct
     2461 gacgccaccg gagagcggtc tcgaagtcat ccgcgcggtc aaagagatcg atccgaatgt
     2521 gttctgcatc ctcgtcagcg cgcacatgag cgtctcgcat gcgctgatgg gcgtccgcgc
     2581 tggcgccgac gacgtattca tcaagcccgt cgccgcgcga caagtgatct cccgcgtcga
     2641 gcaaggtgtc gtcgacgcgc ccaatggcgc tacgcccacg ctgcacgagg tcgagtggga
     2701 gcacatctcg cgcgtgctcc acgaccacaa gggcaacatc agccatgccg ccgagtcgct
     2761 cggcatcttc cggcagtcgc tgcagcgcaa gatccagaag tacgccccgc gcgtgattgc
     2821 caccggcgaa gagcagccct atccggtcgc gccgcccaag ctcgacgagg aagagcagcg
     2881 ggctcccgcg cccgcggcga agaagccaaa ggcgaaggcg cctgcaaagc agagcgcgag
     2941 agcgagcgcg acaccgccgt cgcacagcga atcgccgtcg aagtcaggcg gcaagtcgaa
     3001 gtcccgggcc agcgcgaagt cgcgccgtcg ccattgaccg ctccgccagc ggcggcgacg
     3061 ctccagccat ggaacgtctc gcggatcgcg ttgcttggat cacgggcgga gcgagcggta
     3121 tcggcctcgc catcgcacat cgtctcgccg acgagaaggt gaagctcgtc ctcgtcgata
     3181 tcgagcaagc cgctctcgat gcggccgaag cggcgctgaa ggcaaagggc gcaagcgtgc
     3241 tcgcgattcg cgccgatgtc gcgtcctccg ccgaagtcgc tgccgccgcc gcacgcgcga
     3301 aagagacgtt cggcctcgtt cacatcatcg tcaacaacgc tggcgtcggg ggcaacggcg
     3361 gcccgatgtg gagcatcgcg gaaggcgact ggcagtgggc gctcggcgtc aacctgtggg
     3421 gcgtcatcca cggcatccgc cacttgctgc ccgcgctcat cgagagcggc gaagagggcc
     3481 acgtcgtcaa cacagcatcg atggcaggcc tcacgtcgac gccgttcatg gcgccgtaca
     3541 cggcaacgaa gcacgcggtc gtcgcgatgt cggagtgctt ggccaaggag ctcgaactgg
     3601 cgaagtccaa ggtgaaggtc tccgtgctct gtccgggttt cgtacagacg aacatcaaca
     3661 cgtcagaccg caatcgctct aaggactacg gctcgaagac gcaatccgca ggcgccgaca
     3721 agttccgcga cgtgctcgcg actctcgtga gctcgggcaa acccgccgcc accgtcggcg
     3781 agcaggtcgt cagcgcgatc aagacggaca agttctacat cctgacgcat cccgagatga
     3841 agcccgccat cgagcaccgc atgaagcaga tcctcgagga gcaaccgccc ggaatcgacc
     3901 cgatgatgcg cgcgctgttc ggctcgcagt aactactgaa tctcgacgat gctcgcgttc
     3961 gcgaacgtcg tgtgctccca ctgaccgacc gtcgcggagc ccgccgcgac cggaactgcg
     4021 acagccgcga gcgcgctgcc attccactgc aagcggaaca accccgaggt gcccgccacc
     4081 acgttctctt gcgtgagcag gatgtcgccg atcatcggct cacactcctt cgcggtgacg
     4141 ccgagcagac ggctcgtgcc gaagttcacg ccgaagaagt tctcgcctgg catcacgatg
     4201 tcgatgtctt cgatcgcgac gccgaggttg atcgtggtcg cgtcgccatt cggcgagatg
     4261 atccacatga ggccctcgcc ttccgcgccc gcgatgatct tgccggcgat cgggccatag
     4321 cgcttcggaa cattcggcac gacgatgagg ccctcgagat gcacgccctt cggcaccgtg
     4381 atcggcgtcg gtgtgcccgc cttgttgacg cgccacactt cacccgtcgt cgtcgcgacg
     4441 atcagatcac cgtcgaagat gccggtgcga tcgacataca gcgagccgcg catgagccca
     4501 ttgttcgtgc ccggcagatc gatccacggg tttgtgatca ccgatccgtc cggcgagatc
     4561 ttcacgatct gaccgtcgat gccattgccg gtgaacatcg tgccgacgtc ccagcccgcc
     4621 gggttgtcct tgccgctacg cgcggtcgcg atcttcactt cttcgaccat gcccgcgtag
     4681 tcggagaacg gcagctccat gccatcggct tgaatgcgcg cgaagttgtg tggctgaccg
     4741 ccggtgccgt agttcacgga caggatcacg gtgtgcgtcg gctcgtgata gtcgatgccg
     4801 atcggcgtat tgaacggcgt cgagatcgcg acgaacgtca gcgtaggcgg cgggccgtcg
     4861 ttgaagccgt cgatgtagtt cggcacgccg tccttgtccc aatcggcgag accttcgagc
     4921 gcgtcgtcgt atccgtcgtc gtcgctgtcg gtgtccttgc agtcgggcac accgtccata
     4981 tcggtgtctc gaccgggcgg cgcctcgcag ccctcctcgg tgttcgacag tccgtcgttg
     5041 tcgaagtctg tcgaggcatc gggcggcagc gacgcatcta cgtcatcgcc ggtgccatgt
     5101 gcgtgactcc cacacccaca catcagcagc gtcgcggcga gcagatacct cattagccgt
     5161 atgctcgcat gaaataacga ggtcggcgcg ggcgtttctc taggccatgt atctcctcgt
     5221 agccgtgacg atcgtccgtg cggtcgtcgt cgggcgcgtc gacggaggca cctggacgga
     5281 cgcccccacc gaggctcgga gcgagcaaaa agctgagctc gcggcggtgg ttctcgcacg
     5341 cgatggcaag cgtgacgtcg tgctcgtccc cgagggtgtg acggacctcc gcctcggtgg
     5401 ccgcaagatc gccgcgaagt cgctgcgccc gctcacgccc aagacgatcc gctggtccac
     5461 cgtggagccg cacggctttc gcgcacacga cgccacgaac ggtgcgacga gcgacttcta
     5521 cagcaacgtc tccaccgaat cgaagacgtt cggcacgtgg ctcggctacg accacgtcga
     5581 ttacttcgag cacgtcatga tcgagaacgg cgccgcgatc atcccagcga cgatcgtgcc
     5641 gagcgatacc accgccgtcc agatcgcggg cctcggcacg ctgcgcttca aagtcgaggt
     5701 cgagctcgcc gacggcaccc acgccgcgag cccgggcgcg gacgccaccg acagcttcgg
     5761 catcctgccg tccgtccatc gcgtctcgat ccgctcggcc gatggcttcc tcggctggct
     5821 ctccgcctac ctgctcgtcc ccgaagtctt cggctctgcc gggcccggca agaatcacca
     5881 gaccgagcgc ttcatcggtg cggactgcgc agacgtgatg atcggcgcga tgcgacgcga
     5941 cgggcacaag cttccgtaca cgaacgtcgc gggcctgccc tccgtcacga agaccgtcgc
     6001 gaacgccgcc gagctcgacg agacgggcaa cccatccacc gaaatcgcgg gcgtcaaacc
     6061 cggcgacctc attcgcatcg actacggcgg ctcgctacgc caccacacac cgcgcgcatt
     6121 cgatcacgtc gcagcgctgt gggaagacaa gagcgatccc aagggcccac tcgagggcgc
     6181 cgccgacggc aggctcgacg gcttcgacct cgtcatccac gtcggccatc cacgtctcgt
     6241 catcgagccg ctcgcgcgcc aagcgccggc gacgattgat gtgctccgct ggaaaaacta
     6301 aagccgcagt ctcctgcggc tcatgacaaa acgaacacgc ggtgcgcgtc gattacatgc
     6361 gcgtgccgtc gtagtcggca ggctgcgcga gcgtcgcgtc atccgcctcg tacgtcatcg
     6421 tgccctgcca cgtgccgaac acgagcttgt cgagcttgta gtcgttgttc gtgtccgccg
     6481 cgcgcgacga accgacgagg tgcagcgaca gcttcgagtt ctgatccgcg atctgctcgt
     6541 acagc
//
LOCUS       DATLBZ010000166         4094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3885722, whole genome shotgun sequence.
ACCESSION   DATLBZ010000166 DATLBZ010000000
VERSION     DATLBZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3885722"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..240)
                     /locus_tag="VMZ53_08775"
     CDS             complement(7..240)
                     /locus_tag="VMZ53_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28590.1"
                     /translation="MPPKTSCPVSLTQFLEKAEPLKVSINGQEMLAEVKQFSTGSFGW
                     YMNGKTVVSIDGKAVSVQIGMNLTVVGSKDAER"
     gene            complement(255..1433)
                     /locus_tag="VMZ53_08780"
     CDS             complement(255..1433)
                     /locus_tag="VMZ53_08780"
                     /inference="COORDINATES: protein motif:HMM:NF025606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4344 domain-containing metallopeptidase"
                     /protein_id="HUS28591.1"
                     /translation="MTTRIGVVLLAIGLSAARAAHADGITMVETQGIEVALPEGWAAA
                     NKGKLTILAPTKFKGRAIEVMTFKGAPPATQEAMQKELGDKFTVTMTKEVDRNGIKVV
                     IASANVKTEKGDATLDLLAMPSANGNSVVLMSFVAADQDPLLRKQSGAVLLSARTAGP
                     RISVEFTAPKSKGVTAPPDNYIDAFKKLGPGLDSALILPRPLPIKFQECGFVNAFYSP
                     KTHDITMCHELWTHFTQLFTDAGYKDKDLDEKIRGAMTFTFFHEFGHALHGELDLPVT
                     AKGEDAADEIATLFLAQLGKSGEKIAWAGASWFFIAGDRKEDLGARLYDEHSLHGQRF
                     GSIMCLLYGSKKSNIADPAFMKKMGFDAKRLAKCSRDYQARYKAWNQLLEPYHRKKKK
                     "
     gene            complement(1459..2019)
                     /locus_tag="VMZ53_08785"
     CDS             complement(1459..2019)
                     /locus_tag="VMZ53_08785"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUS28592.1"
                     /translation="MSDDDLVWRLGAHVPGSDYRVFKTSFVDGEHSSGLKKRFSVIEA
                     VDWVNVIALTPKNEVVMLRQYRVGIAKICLEIPGGMCDPGEDARTAAARELAEETGYT
                     SKVWRKLGVVSPNPAIQNNYLHSFLALSAEKTEPQKLEGSEFVDVELMPLDKVTGALK
                     SGQIEHALVVAAFAHLAFSMGDLRAP"
     gene            2092..2715
                     /locus_tag="VMZ53_08790"
     CDS             2092..2715
                     /locus_tag="VMZ53_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HUS28593.1"
                     /translation="MTRARTSETAIARPSEHAARTPEPQTSEVLSAVSHDLRNPLGTI
                     TLGTTLLLNQYGHEARARRPLEMIQRAAARMQDLMERLTDLAGIQVGHLDLDLEDVHA
                     NELIGCGLEGKSVTAEHAIDDVIVRCDKRRVCHVLHMALGDTLRDSGEPVRIHVTRQA
                     SGQALFVISENASWRTVEHYLSHGIIAAHGGELWLEGASLFFTLPEA"
     gene            2712..3083
                     /locus_tag="VMZ53_08795"
     CDS             2712..3083
                     /locus_tag="VMZ53_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS28594.1"
                     /translation="MTMKVLVADDDPEMLELVTNVARRHLHADVTQVSTGHELLDAIA
                     HDAFDLVITDISMPWMTGLQVMHSVRAAGLPTPVVVMTGMQASELPEQVKALGDRARL
                     LHKPFTLQELLAAVGEIAHAA"
     gene            3099..3968
                     /locus_tag="VMZ53_08800"
     CDS             3099..3968
                     /locus_tag="VMZ53_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HUS28595.1"
                     /translation="MLAATTETAIGKSAVRTSLLFERAVRGTVIRRDQLTFDTRFAAA
                     AGGKPEPVGHLFMMLTGRFVSDRGDVVAGPVAFMLGDDEWERPRKDSRTFRTDGPIVE
                     VLQLRVAKEHIRVPIGLAAGALALTPACWDATAAMLGGVPDARRLAALIDALATSGVF
                     AKEMATSVVEEEPERFRRLWEALQPLYQTYGATTSLKQLASSLGMSMRQVGRDAKELS
                     AAFGIGGGYRDALLMMRLRMAVLLLSAPDASVSDVAKLVGYGSPIAMARAFRDAKLPA
                     PSAIQTELAANRI"
BASE COUNT          670 a         1301 c         1334 g          789 t
ORIGIN      
        1 cttcggctag cgctcggcgt ctttgctgcc gacgaccgtc aggttcatac cgatctgaac
       61 cgacacggcc ttgccgtcga tgctgacgac ggtcttgccg ttcatgtacc agccgaagct
      121 gcccgtcgag aactgcttca cttccgcgag catctcttgg ccgttgatcg agaccttgag
      181 cggctcggcc ttctccaaaa actgcgtgag cgatacggga caggaagtct tgggcggcat
      241 tgcgccgcat cctactactt cttctttttg cggtgatacg gctcgagcag ctggttccag
      301 gccttgtagc gagcctggta gtcgcgcgag cacttggcga ggcgcttggc gtcgaagccc
      361 atcttcttca tgaaggcggg gtccgcgatg ttcgatttct tgctgccgta gagcaggcac
      421 atgatcgagc cgaagcgctg gccgtgcagc gagtgctcgt cgtagaggcg cgcgccgagg
      481 tcttccttgc ggtcgcccgc gatgaagaac cacgaggcac ccgcccatgc gatcttttcg
      541 ccgctcttgc cgagctgagc gaggaacagc gtcgcgatct cgtcggcggc gtcttcgccc
      601 tttgcggtga cgggaaggtc gagctcgccg tgcagcgcgt gaccgaattc gtggaagaac
      661 gtgaacgtca tcgcgccgcg aatcttttcg tcgaggtctt tgtccttgta gccagcgtcg
      721 gtgaacagct gcgtgaagtg tgtccacagc tcgtggcaca tcgtgatgtc gtgcgtcttc
      781 gggctgtaga acgcgttgac gaagccacac tcctggaact tgatcggcag cgggcgcggc
      841 aggatgagcg cgctgtcgag gccgggcccg agcttcttga atgcgtcgat gtagttgtcg
      901 ggcggcgcgg tcacgccttt gctcttcggc gccgtgaatt cgacggagat gcgcgggccc
      961 gcggtgcgtg cggagagaag cacggcgccc gattgtttgc gcagcagcgg gtcttgatcc
     1021 gccgcgacga acgacatcag gacgacgctg ttgccgttgg ccgaaggcat cgcgaggagg
     1081 tcgagggtcg cgtcgccctt ttcggttttg acgtttgccg acgcgatgac gaccttgatg
     1141 ccgttgcgat cgacctcctt cgtcatcgtg accgtgaact tgtcgccgag ctccttctgc
     1201 atcgcctctt gcgtcgccgg cggagcgccc ttgaacgtca tcacttcgat cgcgcggccc
     1261 ttgaacttcg tcggcgcgag gatcgtcagc ttgcctttgt tcgccgccgc ccatccctcg
     1321 ggcaacgcga cctcgatgcc ctgcgtctcg accatcgtga tgccgtcggc atgtgcagcg
     1381 cgcgctgcgg acaaaccgat ggcgagtaac accaccccta tgcgtgtcgt catgcgccca
     1441 gcataaaccg ggtggcgatc agggtgcgcg cagatcgccc atcgagaatg caagatgcgc
     1501 gaacgccgcg acgacgagcg cgtgctcgat ctggcccgac ttcagcgcgc cggtgacttt
     1561 gtcgagtggc atcagctcga cgtcgacgaa ctcgctgccc tcgagcttct gcggttcggt
     1621 cttctcggcg ctgagcgcga gaaagctatg gaggtagttg ttctggatcg cgggatttgg
     1681 cgagacgacg ccgagcttgc gccacacctt cgacgtgtac ccggtttctt ccgccagctc
     1741 gcgcgccgcc gcggtccgtg cgtcctcgcc cggatcgcac atgcctcccg ggatctcgag
     1801 acagatcttc gcgatgccaa cgcgatactg ccgcagcatg acgacctcgt tcttcggtgt
     1861 cagcgcgatg acattaaccc agtcgactgc ttcgatcacc gagaaccgtt tcttcaagcc
     1921 gctcgaatgc tcgccgtcta cgaacgacgt cttgaacacg cggtagtcac tgccgggcac
     1981 atgcgcgccg agccgccaca cgagatcgtc atcgctcatg gcctcgacct tatctcacga
     2041 cgctctatca ctgccctgtg gatggcccgt gacgtgcacc gcttccgccc tatgacccgt
     2101 gcgcgaacct cggagacggc gattgcccgt ccgtccgagc acgctgcgag aacacccgag
     2161 ccgcagacct ctgaagtgct ttcggccgtc tcgcatgatc tccgcaatcc ccttggcacc
     2221 atcacactcg gcacgacgct gctcttgaac caatacggtc acgaggcgcg cgcacgcaga
     2281 cccctcgaaa tgatccaacg cgccgcagcg cgcatgcagg atctcatgga gcgcctcacc
     2341 gatctcgccg gcatccaggt cggtcacctc gacctcgacc tcgaagatgt ccacgcgaac
     2401 gagctcatcg gttgcggcct cgaaggtaag agcgtcacgg cagagcacgc aatcgatgac
     2461 gtgatcgtca gatgcgataa gcgccgcgtc tgccacgtac tccacatggc actcggcgac
     2521 acgctgcgcg atagcggcga accagtccgc atccacgtga cgcgtcaggc gagtggccag
     2581 gcgctcttcg tgatcagcga gaacgcgtcg tggcggacgg tcgagcacta tctctcgcac
     2641 ggcatcatcg cggcgcatgg tggcgagctc tggctcgaag gcgcgtcgct gttcttcacc
     2701 ctgcccgagg catgacgatg aaggtgctcg ttgccgatga cgatccagag atgctcgagc
     2761 tcgtgacgaa cgtcgcgcgg cgtcacctcc acgcggacgt cacgcaggtc tcgaccggcc
     2821 acgagctcct cgacgcgatc gcgcacgacg cattcgacct cgtgatcacg gacatctcga
     2881 tgccgtggat gacgggcttg caggtgatgc attcggtccg cgccgcgggg ctgccaacgc
     2941 cggtcgtcgt gatgacgggc atgcaggcgt ccgagctgcc ggagcaggta aaggcgctcg
     3001 gcgaccgcgc gcggctactg cacaagccgt tcacgctgca agagctgcta gcggccgtcg
     3061 gcgagattgc acacgcggcc tagtgttacg atccgtcggt gctcgctgcg actacggaga
     3121 ctgcgatcgg caagagcgcc gttcgcacga gcctcctctt cgagcgcgcg gttcgcggca
     3181 cggtcatccg tcgtgaccag ctgacattcg atacgcggtt tgcggcagcg gcgggcggta
     3241 agcccgaacc ggtcggtcat ctgttcatga tgctcacggg ccgcttcgtg tcggatcgcg
     3301 gggacgtcgt cgcgggcccg gtcgcattca tgctcgggga cgacgaatgg gagaggccgc
     3361 gcaaggactc gcggacgttt cgcacggacg gacctatcgt cgaggtgctg cagctgcgcg
     3421 tcgcgaagga gcacatccgc gtgccgatcg gtcttgccgc cggggcgctc gcgctgacgc
     3481 ccgcgtgttg ggatgcgacg gcggcgatgc tgggcggggt gcccgatgca cgacgtctcg
     3541 cggcgctgat cgatgcgctc gcaaccagcg gcgtgttcgc gaaggagatg gcgacgagcg
     3601 tggtcgagga agagcccgag cgctttcgcc ggctgtggga agcgctgcag ccgctgtatc
     3661 agacgtatgg ggcgacgaca tcgctcaagc agctggcatc gagcctcggc atgtcgatgc
     3721 ggcaagtcgg gcgtgatgcg aaggagctat cggccgcgtt cgggatcggc ggcggctatc
     3781 gcgatgcact gctcatgatg cgactgcgca tggcggtgct cctgttgtcg gcgcccgatg
     3841 cgtcggtctc tgacgtcgca aagctcgtgg gatatggcag cccgatcgcg atggcgaggg
     3901 cgtttcgcga cgcgaagtta ccggcgccga gcgcgatcca gaccgagctc gcggcgaata
     3961 gaatctgacg tgcgttcgat ccgtgtggac ccttgtgggc cggcgggtta ctgagggttc
     4021 gtggccacgc ttcgcaccgc gctcgctgtt ctgatcgcgc tcgcgacaag cgcgcatgcg
     4081 ggcggttttg gcat
//
LOCUS       DATLBZ010000167        17761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6788578, whole genome shotgun sequence.
ACCESSION   DATLBZ010000167 DATLBZ010000000
VERSION     DATLBZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17761
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6788578"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VMZ53_08805"
     CDS             <1..367
                     /locus_tag="VMZ53_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HUS28596.1"
                     /translation="RPADPDATVRSLSGGNQQKIVIARELSRPQLRTVLAVQPTRGVD
                     LGAVARIHDGLRTAARSGAGVLVISADLDELLALCHRVIVLLRGRIVGERAGAALHAS
                     DARTALGKLMTGASEGEAA"
     gene            801..1844
                     /locus_tag="VMZ53_08810"
     CDS             801..1844
                     /locus_tag="VMZ53_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS28597.1"
                     /translation="MSPRVVREIQAALIALLVSVLIGSVLMLIAGKAPGTVWWAMVSR
                     TASDPYALGQVLYKATALILTGLAVSVALDAGLFNIGGEAQLGAGVIACSIVGAALPA
                     GTPAVVAIVLCVVAAAAAGGAIGFVIGVLRVTRDAHEIITSIMLNAIVAGVALWLGNV
                     ALFQGGTTRGTAIAPGAELPQLGFGGSAANASLFLALAIVALVWWLRGRTTWGRAWLA
                     VGRNPEAARTVGIDVGRVRIIVMTGAGALAGLAAANFVLGHKHAFEEGLGRGTGLLGI
                     SAALLGRSHPVGVVVAALVLGFLSAGGLNVGDLVPKELTEMLQGVVVLSVACAVPWVQ
                     RRAELQQLGRGVA"
     gene            1841..2710
                     /locus_tag="VMZ53_08815"
     CDS             1841..2710
                     /locus_tag="VMZ53_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS28598.1"
                     /translation="MIASMFTLAFLASIIRISVPYVLAALGGSVTERSGMVDLALEGK
                     LLFGAFAAAAVGHATNSALAGIVAAMAVGMLVASLQALCALVFEADQAIVGIAINLLA
                     LGGTRFLLQLLFHEGSNSPPAPSYGTDVVENPIVWLAVLAALLVPFAVWRTRWGLRLR
                     ASGDRPESLLAVGVRPNEARLWAALVGGALTGAGGAQLSLAVGGFSADMTSGRGYIAL
                     AMVILGGWRPAYAVLACVGIAAAEALNILLQTQSVAIPRELAPLLPYVLTLVVLAVWG
                     SKGRKAPASLGKL"
     gene            2720..3574
                     /locus_tag="VMZ53_08820"
     CDS             2720..3574
                     /locus_tag="VMZ53_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28599.1"
                     /translation="MRRAVLLLIIGGCGRIGFSPISQTDAADGPAPDAVAPFSTPVML
                     TMLSSAFEDDDPTLTDDELEIYFSSDRPGSQASDIWRSLRASTSDPWAAPMNVAQLNT
                     AADEQTPELSADGLTMFLARDTSSSTMIDIFVSTRATRNQPWSTPVLVAELASGSDDF
                     APATSPDQLAMVFTRGAVTVDAKLFLTTRTSTTAAWGTPVAIVDAPGELENQAWLGDA
                     SIYYTINVGAAEQRIARAARTAPGSFAAPQTIPEIDLPGSESDPWLSVSERAIYFEHE
                     DDLYMATR"
     gene            complement(3576..4430)
                     /locus_tag="VMZ53_08825"
     CDS             complement(3576..4430)
                     /locus_tag="VMZ53_08825"
                     /inference="COORDINATES: protein motif:HMM:NF015754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HUS28600.1"
                     /translation="MMRMLLLVTALVTVVGCGKKKDASPGAGPGSGPGSTAVVATPPS
                     APGSAQAPNVDAKPTACDQAAKDVPGNEGTSWFMQCPAGCAEGTVWGTDVYTDDSELC
                     PAAVHAGAITAKDGGVVQVTWTPGQPTYIGSVRNGITTSDYASWGRSFFVQKIDATGK
                     PTTPAPPLMTAKNTAHLSCKMDASPLTGDAGAKWNVDCPAGCEGSHVWGSGPYTGDSN
                     VCSAAIHAGVIDAKGGGFVVTIGGKVPSFKGSAKNGITTSDYEAYDRTYTIEAVAPAS
                     PPAPAPKP"
     gene            complement(4448..6163)
                     /gene="argS"
                     /locus_tag="VMZ53_08830"
     CDS             complement(4448..6163)
                     /gene="argS"
                     /locus_tag="VMZ53_08830"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HUS28601.1"
                     /translation="MPSHHTPLHKAAAEAVSSATGVDATDIKVDVPKPEMGDLAVGCF
                     AIAKAKGTNPAQLAKEIVAAFKPTDLLASATAAGPFVNFKVNRAAAFKWLIGASLGHS
                     LLSRDIGAGKTICIDYGSPNISKHLAYHHIRSTTIGHALAQTFRALGYKVIGINHLGD
                     WGTTHGALIAAFKKWGPFSAPMTVAELNALYVKFTEAKKQDPALDDEARAWFKKLENN
                     DPEARALWQQFRDVSWAEYQEVYDLLGIGYDEVRGESAYEPDLPRVMEELKAKGLVVE
                     SEGAEVVFLDGEKTPILLRKQDGTTLYATRDVAAAEYRWNTYHPTRSLYVVDRGQSLH
                     FRQLFKLLKVAGHDWAGVCEHIPFGLVRMGGKKTSTRLGNVVLMREVFQIAEDEVRER
                     VAQISAHLPPDVVTTVARQVGIGAVVFANLASQREKDIDFDIDKAVSLDGDSGPYLQY
                     SYARCASIARKAGVTVTSVDGVDFTKLTHDAEWAVAKKLVEYPEIVGRSADAAEPHVI
                     AHYLLELAGEFSRWYTLGNGDASLRVLVEDAEVRRARLALVAAVQATMKQGLSLLGLA
                     APEQM"
     gene            6269..7297
                     /locus_tag="VMZ53_08835"
     CDS             6269..7297
                     /locus_tag="VMZ53_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS28602.1"
                     /translation="MSKIVLCVDDSATMQQVADITFRGTDFQYVGARSVDEALQKAKG
                     QKPAVVLADAAMPGKNGYDLCLAIKNEMADVPVVILVGNGAPYDAAKGTQVGADANLP
                     KPWDTQTMLEKVTEIVGKGATAKPGQAAASAPAAAAPAASKPAATPAPGPAAAAAAAA
                     AAAGNQPPRSATIMGMPTIKMPAGVATPVTATVPSAPKTTAMAVPPMAPQPATTTPGA
                     APGLRPPASPSASASASSSAPAPSTSSSSASSASSSIPGVLGMNRNPMVAGNATKRSQ
                     LVEATLNKMAARIAEISGLNQGSPELAALLKLSTEVIERIVWEVVPDLAEQIIRENLN
                     DLAAARRS"
     gene            7455..7982
                     /locus_tag="VMZ53_08840"
     CDS             7455..7982
                     /locus_tag="VMZ53_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28603.1"
                     /translation="MRNRSLLISMVATLALSGCIIKGRARTGSGGPPPPPPPSGGTAT
                     VETAPPPPSQPPPSQPASSQPPPPPQNHGQERSAEVHERNEERKAQHDADKAEKKADK
                     AEAKADKMEAKAEKTEAKAEKAEAKGQDKKAEKLEQKAEKQEKAAEKQEKKADKLDDK
                     ADKAQDKADKKAGKK"
     gene            8080..10929
                     /locus_tag="VMZ53_08845"
     CDS             8080..10929
                     /locus_tag="VMZ53_08845"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HUS28604.1"
                     /translation="MSTSTALPKQYDPSEVEPRWLSFWLENGFFHADEAAPNVPYSIT
                     LPPPNVTGSLHIGHALGSSMQDILVRWKRMQGFNAMWMPGIDHASIAVHVLLEKDLKR
                     REQKTRFELGRDEFMRRAWAWKERSGNRISEQEKLMGFSLDWGRERFTMDEKSNRAVR
                     EAFVRLYDEGLIFRAKRMINWDPASETVVSDLEVDTAEESGSLWQIKYPLVGGGGEII
                     VETTRPETMLGDTAVAVNTNDDRYKHLHGKMVELPLTGRQIPIVPFDLNRDGKSWPDP
                     AFGSGAVKITPAHDPNDYDASQIANLPVLQVIDPKGRMTAPAPAKYVGMSVDDARKAV
                     VADLEAGGFLGDVKPYKVPRGRSQRSGAVIEPMLMDQWWVKAAPLAEKCVAAVEAGKT
                     KFVPELWTKTYMHWMTNIKDWCISRQLWWGHRIPAFYCQTESCGHTTVSREDVTACAK
                     CGGATKQDEDILDTWFSSALWPFSTLGWPDKTRELSTFYPNNVLVTGPDIIFFWVARM
                     MMMGIHFMGKVPFRTVYLTSIVTDENGDKMSKTKGNVIDPLDVVHGATLQVLLDRVDV
                     EKPPDPEKVKNAVKKHFAKGIPAMGADALRFALAALNTGSSRIRLSIERVEGYRNFIN
                     KLWNASRFALMNLDGYDPERFEAQLATPAGKAQLGMPEKWILSRLQATSAAVDASLEA
                     FRFSDAANAIYHFVWDELCDWYIELAKPHLHKQDGLHQDNVKAARRHIVQGVLATTLE
                     TTMRLFHPFAPFVTEEIWQKLPKPPQLPGSLMITVFPRGDQQWVDPGAEAEMKLLQDV
                     VSTARMLKQTYGIAPAQSIAVELRITADAPRAIIEKYKEMVEARAKVTSTITSASSGA
                     PSPGTAKAFIGSDVQVLMPLGGLIDAPTEKARISKDIGKCEKEIGALEKKLANQDFLS
                     RAPEEVVAEIRQRLEDEKSRMKLLKDALETLGEAK"
     gene            10926..11564
                     /locus_tag="VMZ53_08850"
     CDS             10926..11564
                     /locus_tag="VMZ53_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HUS28605.1"
                     /translation="MTSGLSRDRAALLVVDIQERLAAVMQPAVLEQVVRNTVVLIETA
                     NRLGLPIAVSQQYPKGLGATVPAIEEALRAVTTPAGWPGGDRVYRFDKTVFAATASEQ
                     FAGATRDPLQRDQWIVAGMEAHICVYQTARGLVTRGLQAHVVSDAVCSRTKANWKIGC
                     KLVERLGDARGLDATAPVGGGGAVLTSTEVVVFDLLGRAGTDDFKALSRLIK"
     gene            11576..12478
                     /locus_tag="VMZ53_08855"
     CDS             11576..12478
                     /locus_tag="VMZ53_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HUS28606.1"
                     /translation="MAGSTGEVLKSLAVNAVIATSKGIAAAMTGSGALLAETLHSFAD
                     CGNQLLLLVGIKRSARPPSPKYPLGQGREMYFYSFVVALLLFSGGGVFSVYEGIHKLK
                     HPEKVGNITVGLVILGLSILLEGWSTWGNLKEMKKRRKDKPLLKYLRDSKDSDLIVVF
                     GENSAAVFGLVLALLSLIVAKQTNDGRWDAIGSIAIGVVLVGVALFLAREIKSLLVGE
                     AADPELEAAMKQLAVDDPNVDEMLRLLTVQQGPGEIVVAAKLRFRAGLDTKQLVDAIN
                     GFERELKKRVPEVRWSFIEPDHTD"
     gene            complement(12485..13552)
                     /locus_tag="VMZ53_08860"
     CDS             complement(12485..13552)
                     /locus_tag="VMZ53_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28607.1"
                     /translation="MRRAVMLLLGGCAQSLGLSQPVLVDATNDPDSPPADMQSRCAPR
                     RVGATLFGSDVSMTAVKTTEGFVVLAIERDQGNLRLQRYTTALEPVDQVPLVVNLAGS
                     SVAGLADGNRLVIGVLDVDKTRMASIPLASPSVATSGTTLTDAIAEGDAILPTQSGNF
                     DVYAAHGTGYFHYGVTSDLNGSSAGPGGGTGGPSAGCVAAADNGDIALACPKLSVGEA
                     YCFTTHISPNGTALGGFPEACPNISLVQRNGVAIAVYEKGGGLQLSSWANTASPGTPV
                     PIATIPNATQPLIDVDPSGIVIAMRRQGSVYTTRDSTAAPYAPVRLEGMPSPLGFAIA
                     RGTTDTVLFVAAADGIYRACI"
     gene            13666..14955
                     /gene="ccrA"
                     /locus_tag="VMZ53_08865"
     CDS             13666..14955
                     /gene="ccrA"
                     /locus_tag="VMZ53_08865"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HUS28608.1"
                     /translation="MSTAIASRSNDLYELGDVPELGVVPARMLAQVIRAERFGEPRDA
                     FKEEPLPIPELGPRDVLVYVMAAGVNYNNVWAAMGIPIDVIKGRQKRWGAKEDFHIGG
                     SDASGVVWAVGKDVKNVKVGDDVVVHCGMWEPDDPWVLAGKDPMFAPTQQIWGYESNW
                     GSFAQYTKVQDHQCLPKPPHLTWEDAAAYMLVGATAYRMLHGWSPNVIGPGDPVLIWG
                     GAGGLGSMAIQICKAAGARPVAVVSGDSKNEFCKKLGAVGVIDRRKFDHWGKLPRWSD
                     DIEYAQWLKGARAFGAAFWEALGEKKNPTIVFEHPGETTIPTSCFMVETGGMVVICAG
                     TTGYNATLDLRYHWMRQKRLQGSHFANDDQCKGFNDLVLAGKIDPCMSRVFKFDETGE
                     CHQLMRENQHPSGNMSILVNAPRTGLRTLDDVRNAKR"
     gene            14955..15386
                     /locus_tag="VMZ53_08870"
     CDS             14955..15386
                     /locus_tag="VMZ53_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS28609.1"
                     /translation="MSTRPFRVLGLQQIAIGGPSKDALRRLWIDLLGVPQTGTFKSER
                     ENVDEDILAVAGVEIDLMQPIDPEKKPKVHEPRLNHIGLWIDDLPAAVTWLEGQGLTF
                     TPGGIRKGAAGHDVCFIHPKSAEGVLIELVQAPPDVIAAAK"
     gene            complement(15388..16200)
                     /locus_tag="VMZ53_08875"
     CDS             complement(15388..16200)
                     /locus_tag="VMZ53_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28610.1"
                     /translation="MNRCVGIVAIFVCACGPTTREPAGQPDAATSPDSATVVDTPPPQ
                     ASGPVDVVITADNAYSFGYGTADTITTFIQGQRAQTAGQIWNCGEGPEQYTVAAADAP
                     DGAYLYVVTWDDLAVTQGVLAQFKRDTGTVLTGDDRFEVCATGLDYSGGANATTGPAQ
                     SIVNAEIAKCNAASGDTNTSKGWVSTTGASAGTPSALGRLAVGEANDAAAGTFPIVCQ
                     PGQTASPGIDSAAHWMWYDPSDASSSDAFHSTGVNRTKAFLIFRLAAAAIIL"
     gene            16289..17182
                     /locus_tag="VMZ53_08880"
     CDS             16289..17182
                     /locus_tag="VMZ53_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28611.1"
                     /translation="MGRWLLVLAVVGACNQAFDLEPTLGPDSDGDGIADGDDNCLLVG
                     NHDQANEDHDALGDACDPCVAGSQLGIDGDRDGVDDACDACLEGRNSDEDDDGVLDGC
                     DDCPADADADQLDSDGDGVGDACDIGPAVQNTRLLFDGFAPPVPHWDTGFWEWRAVDG
                     SFGPVTPIRSKYDGAWNPDIVVPGRRWYLDTSIGIFADSAFDGAYIKLVALTSLGGFT
                     ARDCGIQFNQGQWRDPVTSTVIVVGAATRLRLVDTGAVVQCWINGTLMHTKPSTDPTT
                     AWLPFLQTNTQSEFRWLDVVP"
     gene            17161..>17761
                     /locus_tag="VMZ53_08885"
     CDS             17161..>17761
                     /locus_tag="VMZ53_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28612.1"
                     /translation="MARRRPVIAGGALVLALSACNAAFGLVPTLGPDSDGDGHDDGHD
                     NCPLVVNPDQADRDDDTLGDLCDPCDGPQAGTDNDHDGVDDGCDACLTGRNTDEDHDA
                     YLDGCDACPGTSDDQADDDGDGIGNACDADPGAQNHRVFFDGFDPPLSDWRAWLTTWH
                     PTEGGGFEPVGISPGGTGAWHPASVVTGNEFWAEAVIDVP"
BASE COUNT         2983 a         5741 c         6059 g         2978 t
ORIGIN      
        1 ccgcccggcc gaccccgacg cgaccgtccg ctcgctgtcc ggcggcaacc agcaaaagat
       61 cgtcatcgcg cgcgagctct cgcgcccgca gctccgcacg gtgctcgccg tccaaccaac
      121 gcgcggtgtc gacctcggcg cagtcgcccg catccacgac ggactgcgca ccgccgcccg
      181 ctccggcgcc ggcgtcctcg tcatcagcgc cgacctcgac gagctgctcg cgctctgcca
      241 ccgcgtcatc gtcttgctcc gcggccgcat cgtcggtgaa cgcgccggtg ccgccctcca
      301 cgcgtccgac gcccggacag ccctcggcaa gctcatgacg ggcgcatcag agggcgaggc
      361 ggcatgatcg ccggcgccgc tgcttcgagg tgcgcgccca atgtggggtg tcttttcgaa
      421 acccgtcatg acgtcgtact gacggataac tgcgcgaaac aagaaaccgc accctcgcaa
      481 tcgggaaacc gaccgattgc tgcaggcggt gaccgatccc gatgccgatg cggaggccga
      541 accgcggcga cgtgcgcggt gccgatgtcg tgcggtcgga gtgtcgaggg aagcgatcgt
      601 cgacgcgcga atgtcgtgag tgcgacgagc ggtcgcgcac ttgcctgtac tgacgccgtc
      661 atgacgggtt tcaaaaagac accccatgat cagggtggtc ggccgaagtc gcgaggcgca
      721 ggagctgggg catctgcgcg ggcactggag ccgagccata tggtcgcgcg ggggcgtgcg
      781 tgagtccggg tgtggttcgc gtgagtccgc gcgtcgttcg cgagattcag gcggcgttga
      841 tcgcgctgct cgtgtcggtg ttgatcggca gcgtgctgat gctgattgcg ggcaaggcac
      901 cgggcacggt gtggtgggcg atggtctcgc gcacggcgag cgacccgtat gcgctggggc
      961 aggtgctgta taaggcgacg gcgctcattc ttactgggct cgcggtgtcg gtcgcgctcg
     1021 acgcgggact cttcaacatc ggcggcgagg cgcagctggg tgcgggcgtc attgcgtgct
     1081 cgatcgtcgg tgcggcgttg cccgcgggga cgccggcggt cgtcgcgatc gtcttgtgcg
     1141 tggttgccgc cgcggcggcg ggcggcgcga tcggatttgt gattggcgtg ctgcgcgtga
     1201 cgcgcgatgc gcacgagatc atcacgtcga tcatgttgaa cgcgatcgtt gctggcgttg
     1261 cgctgtggct cggcaacgtc gcgctctttc agggcgggac cacgcgcggg acggcgattg
     1321 cgccgggcgc cgagctgccg cagctcgggt ttggtggcag cgccgcgaat gcatcgctct
     1381 ttttggcgct cgcgatcgtt gcgctggtgt ggtggctgcg cgggcggacg acgtggggga
     1441 gggcgtggtt ggcggtcggg cgcaatcccg aggccgcgcg cacggtcggc atcgatgtcg
     1501 ggcgcgtgcg gatcatcgtg atgactgggg ccggtgcgct tgcggggttg gccgccgcga
     1561 acttcgtgct cgggcacaag catgcgtttg aagaagggct tggccgcggg acggggttgc
     1621 tcggcatttc ggccgcgttg cttggccgct cgcatccggt tggcgtcgtg gtggctgcac
     1681 tcgtgctcgg atttttgtcg gcgggtgggc tcaacgtcgg cgacctcgtg ccgaaggagc
     1741 tcaccgagat gctgcagggc gtggtcgtgc tcagcgtcgc gtgcgccgtg ccgtgggtgc
     1801 agcgccgcgc ggagctgcag cagctgggga ggggcgtcgc gtgatcgcga gcatgttcac
     1861 gttggcgttt ctcgcgagca tcatccgcat ctccgtgccg tacgtgcttg ccgcgctcgg
     1921 cggcagcgtc accgagcgaa gcggcatggt cgacttggcg ctcgagggca agctgctgtt
     1981 cggcgcgttt gcagcggccg cggtcgggca tgcgacgaac agtgcgctcg cgggaattgt
     2041 cgcggcgatg gcggtcggga tgctcgtcgc ctcactgcag gcgttgtgcg cgctcgtgtt
     2101 cgaggcggat caggcgatcg tcggcatcgc gatcaacttg ctcgcactcg gcggcacgcg
     2161 ctttctgttg cagctgctgt ttcacgaggg ctcgaactcg ccgcccgcgc cgtcgtacgg
     2221 caccgacgtc gtcgagaatc cgatcgtgtg gctcgcagtt ctcgccgcgc tcctcgttcc
     2281 gttcgcggtg tggcgcacgc gttggggcct gcgcctccgc gcgtcgggcg atcggccgga
     2341 gtcgctgctt gcggttggcg tgcggccaaa tgaagcgcgg ctgtgggctg cgctcgtcgg
     2401 tggcgcgctt accggagcgg gtggggcgca gctctcgctc gcggtcggcg gcttctccgc
     2461 tgacatgacg agcggccgcg gctacatcgc gctcgcgatg gtgatcctcg gcggttggcg
     2521 tcctgcgtat gcggtgctcg catgcgtagg gattgcagcg gcggaggcgc tgaatatttt
     2581 gctgcagacg cagagcgtgg cgattccgcg cgagctcgcg ccgctgctgc cgtacgtgct
     2641 gacgctcgtc gtgctcgcag tgtggggcag caagggacgc aaggcgccag cgtcgctcgg
     2701 caagctatag tcgggctgca tgcggcgcgc agtcttgctg ctgatcatcg gcggatgcgg
     2761 gcgcatcggc ttctcgccga tctcgcaaac cgatgctgcg gatgggccgg cgcccgacgc
     2821 ggtcgctccg ttctcgacgc ccgtgatgct gacgatgctg agctccgcgt tcgaagacga
     2881 cgacccgacg ctcaccgacg acgagctcga gatctatttt tcgtctgatc ggccgggcag
     2941 tcaggcgtcg gacatctggc ggtcgctgcg ggcgagcaca tcggatccgt gggcagcgcc
     3001 gatgaatgtc gcgcagctca acaccgcggc cgacgaacaa acgccggagc tgtccgccga
     3061 tggcctgacg atgttcctcg cgcgggacac ctccagcagc accatgatcg acatcttcgt
     3121 gtcgacccgc gcgacccgta accaaccgtg gtccacgccg gtgctcgtgg ccgagctcgc
     3181 gagcggaagc gatgactttg cgcccgcgac gtctcccgat cagctcgcga tggtgttcac
     3241 gcgcggagcg gtcaccgtcg acgcgaagct attcctgacg acgcgcacct cgacgactgc
     3301 ggcgtggggc acacctgtcg cgatcgtcga tgctcctggc gagctcgaga accaagcatg
     3361 gctcggcgac gcgagcatct actacacgat caatgtgggc gcggccgagc agcggatcgc
     3421 acgcgctgcg cgcaccgcgc cgggctcgtt cgcagcaccg cagaccatcc ccgagatcga
     3481 tttgccgggc tcggaaagcg atccatggct ctcagtctcc gagcgcgcga tctacttcga
     3541 gcacgaagac gatttgtaca tggcgactcg ctgaatcacg gcttgggcgc cggcgcaggc
     3601 ggcgacgcgg gcgcgacggc ctcgatcgtg tacgtgcgat cgtacgcctc gtagtcgctc
     3661 gtcgtgatcc cattcttcgc gctgcctttg aacgagggca ccttgccgcc gatcgttacg
     3721 acgaaccctc cacccttcgc gtcgatcacg cccgcgtgaa tcgcggcgga gcagacgttc
     3781 gaatcgccgg tgtacgggcc cgagccccac acatgcgaac cttcgcaacc cgcaggacag
     3841 tcgacgttcc acttcgcgcc ggcgtcgccc gtcagcgggc tcgcgtccat cttgcacgag
     3901 agatgcgcgg tgttcttagc cgtcatcagc ggtggtgccg gcgtggtcgg cttgccggtc
     3961 gcatcgattt tctgcacgaa gaagctgcgg ccccacgagg cgtagtcgga cgtcgtgatg
     4021 ccgttgcgca ccgagccgat gtacgtcggc tggccgggcg tccacgtgac ctgcacgacg
     4081 ccgccgtctt tcgcggtgat cgctccggcg tggacggccg ctgggcacag ctccgagtca
     4141 tcggtgtaga cgtcggtgcc ccagacggtg ccttccgcgc atccggccgg acactgcatg
     4201 aaccacgacg tgccctcgtt gccggggaca tccttcgctg cttgatcgca cgccgtcggc
     4261 ttggcgtcga cgttcggagc ctgcgcgctg cccggcgccg acggcggcgt cgcgacaacg
     4321 gcggtggagc ctggtcccga gcccggaccc gcgcccggcg acgcgtcctt cttcttgccg
     4381 caaccaacaa cagtgacgag agcggtgacc aacaacaaca tacgcatcat gcgcgtactc
     4441 taatcgatca catctgctcg ggtgcagcga ggccgagcag agaaaggccc tgcttcatcg
     4501 tggcctggac cgcggcgacg agcgcgagac gcgcgcgccg gacctcggca tcctcgacga
     4561 gcacgcgcag tgatgcatcg ccgttgccga gcgtgtacca ccgcgagaac tcgccggcga
     4621 gctcgagcag gtaatgcgcg atgacgtgcg gctccgcggc gtcggcactg cgcccgacga
     4681 tctccggata ctcgacgagc ttcttcgcga cggcccattc ggcatcgtgg gtgagcttcg
     4741 tgaagtcgac gccatcgacg ctcgtcacgg tgacgcccgc cttgcgtgcg atcgatgcgc
     4801 accgcgcgta gctgtactgc agatatggac ccgaatcgcc gtcgagcgag accgccttgt
     4861 cgatgtcgaa gtcgatgtcc ttctcgcgct gcgacgcgag gtttgcgaac acgaccgcgc
     4921 cgatgcccac ctggcgtgcg accgtggtga cgacatcggg cggcagatgc gcgctgatct
     4981 gcgcgacgcg ctcgcgcact tcatcctccg cgatctggaa gacctcgcgc atcagcacga
     5041 cgttgccgag ccgcgtgctc gtcttcttgc cacccatgcg aacgagcccg aacggaatgt
     5101 gctcgcacac gccggcccag tcgtggcccg cgaccttcag gagcttgaac agctggcgga
     5161 agtgcaggga ctggccgcga tcgacgacgt agagagaccg cgtcgggtga tacgtgttcc
     5221 accgatactc cgcggcggcg acatcgcgcg tcgcatagag cgtcgtgccg tcctgcttgc
     5281 gcagaaggat cggcgtcttc tcgccgtcga ggaacacgac ttcggcgcct tcgctctcga
     5341 cgacgagccc cttggccttt agctcttcca tcacgcgcgg caggtccggc tcgtaggcgc
     5401 tttcgccgcg cacctcgtcg tagccgatgc cgaggagatc gtagacctct tggtactccg
     5461 cccacgagac gtcgcggaac tgctgccaga gagcgcgcgc ttccggatcg ttgttctcga
     5521 gcttcttgaa ccaggcgcgt gcttcgtcgt cgagcgcggg atcctgcttc ttcgcctcgg
     5581 tgaacttcac gtagagggcg ttgagctcgg cgaccgtcat cggagcggag aacgggcccc
     5641 acttcttgaa cgcagcgatc agcgcgccgt gggtggtgcc ccaatcgccg aggtgattga
     5701 tgccgatgac cttgtagccg agcgcgcgaa acgtctgcgc gagtgcgtga ccgatcgtcg
     5761 tcgagcggat gtggtgatac gcgaggtgct tcgagatgtt cggcgagccg tagtcgatgc
     5821 agatcgtctt gcccgcaccg atgtcgcgcg acaggagcga gtggccgagc gacgcgccga
     5881 tgagccactt gaacgcagcc gcacgattga ccttgaagtt gacgaacggg cccgctgccg
     5941 ttgcgctcgc gagcaggtcg gtcggcttga acgcggcgac gatctccttc gcgagctgcg
     6001 cggggttcgt gcccttcgcc ttcgcgatcg cgaagcaacc gacggcgaga tcgcccatct
     6061 cgggcttcgg gacgtcgacc ttgatgtccg tcgcatcgac gccggtggcg ctgctgaccg
     6121 cttcggccgc agccttgtgc aatggcgtgt gatgactggg catcgggctg cgcaagctag
     6181 caccgaaaga ttactgtttc taggcagttg gtttgacgct ctgtcgaagc tcttgctact
     6241 gtagcgtcga gcatttggga ggcacctcgt gagcaagatc gttttgtgcg tcgacgacag
     6301 cgcgacgatg cagcaggtcg cggatatcac tttccgcggc acggactttc agtacgtcgg
     6361 tgcgcgctct gtcgacgagg ccttgcagaa ggccaagggg cagaagccgg cggtagtcct
     6421 cgctgacgcc gcgatgccgg gcaagaacgg ctacgacctc tgcctcgcca tcaagaacga
     6481 gatggcggat gtgccggttg tgatcctcgt cggaaacggc gcaccctacg atgccgcgaa
     6541 gggcacgcag gtcggcgccg atgcgaacct cccgaagccg tgggacacgc agacgatgct
     6601 cgagaaggtc accgagatcg tcggcaaggg cgcgaccgcg aagccgggac aagcggctgc
     6661 gtctgcaccg gctgcggcag caccggctgc ttcgaagcct gcggcgacgc ctgcgccggg
     6721 acctgcggcg gcggctgctg cggctgcggc tgcggcgggc aaccagcctc cgcgctcggc
     6781 gacgatcatg ggcatgccga cgatcaagat gcctgcgggc gtcgcgacgc cggtcaccgc
     6841 gaccgtgccg tctgcaccga agacgaccgc gatggccgtg cccccgatgg cgccgcaacc
     6901 cgcgacgacc acgcccggcg cagcacccgg tctccgtccg ccggcgtcgc cgtccgcgtc
     6961 ggcgtccgca tcttcgtcgg caccagcgcc ttcgacttcc tcttcgtccg cttcctccgc
     7021 ctcgtcgtct atccccggag ttctcggcat gaaccgtaat cccatggttg ctggcaatgc
     7081 cacgaagcgc tcgcagctcg tcgaagcgac gctcaacaag atggcggcgc gcatcgccga
     7141 gatcagcggc ctcaaccaag gctcgccgga gctcgctgca ctgctgaagc tctcgaccga
     7201 agtcatcgag cggatcgtgt gggaagtcgt tccggatctc gccgagcaga tcatccgcga
     7261 gaacttgaac gacctcgctg ctgctcgtcg tagctagcga tcgcgcggca gctcgctgcg
     7321 cgattgcgat gtgctccagg gtgcgcgtga ctgtcgtcag atcgtgacga tcgtccgagt
     7381 gccaggacag gctgcggggc aagttgcccc gcctacctcg cgctcacgcg caaagtctgt
     7441 ctacgatcct ggccatgcga aatcgctctc tattgatctc catggtcgcg acgctcgcgt
     7501 tgtcgggctg catcatcaaa ggcagggcgc gtacgggctc gggcggaccg ccgcctccac
     7561 cgccgccgtc gggcggaacc gcgacggtcg agaccgcacc gccgccgccg tcgcagccgc
     7621 cgccgtcgca gccggcgtcg tcgcagccgc cgcctccgcc gcagaaccac ggccaggagc
     7681 gttcggccga ggtccacgag cgcaacgaag agcgcaaggc gcagcacgac gccgacaagg
     7741 ccgagaagaa ggccgacaag gccgaagcga aggccgacaa gatggaggcg aaggccgaga
     7801 agaccgaggc gaaggccgag aaggctgaag cgaagggcca ggacaagaag gccgagaagc
     7861 tcgagcaaaa ggccgagaag caagagaagg ccgccgagaa gcaggagaag aaggccgaca
     7921 agctcgacga caaggccgac aaggcgcagg acaaggccga caagaaggcc ggtaagaagt
     7981 gaagcgaaac gcgcggcgcc tcatcaagta gcgacgcgtt gtagcttcgg gagcgcgctg
     8041 cgcgaatgac gcgccgcgtg ctagcgtccg tcgacagcta tgtccacgtc gacggcgctc
     8101 cccaagcagt acgacccttc ggaggtcgag cctcgctggc tcagcttctg gctcgagaac
     8161 ggtttcttcc acgctgacga agccgcgccg aacgttccgt attcgatcac gctgccgccg
     8221 ccgaacgtaa cgggctcgct gcacatcggt cacgcgctcg gctcgtcgat gcaggacatc
     8281 ctggtccgct ggaagcggat gcagggcttc aacgcgatgt ggatgccggg catcgatcac
     8341 gcatcgatcg ccgtccacgt gctgctcgag aaggatctca agcgccgcga gcagaagacg
     8401 cgcttcgagc tcggccgcga cgagttcatg cggcgcgcgt gggcatggaa agagcgcagc
     8461 ggcaatcgca tcagcgagca agagaagctc atgggcttct cgctcgattg gggtcgcgag
     8521 cgcttcacga tggacgagaa gtccaaccgc gccgtgcgcg aagcattcgt gcggctctac
     8581 gacgagggcc tcatcttccg cgcgaagcgg atgatcaact gggatcccgc gagcgagacc
     8641 gtggtctccg atctcgaggt cgacaccgcg gaagagagcg gctcgctgtg gcagatcaag
     8701 tatccgctcg tcggcggcgg tggcgagatc atcgtcgaga cgacgcgacc ggagacgatg
     8761 ctcggcgata cggccgtcgc ggtgaacacg aacgacgacc gctacaagca tctgcacggc
     8821 aagatggtcg agctgccgct cactgggcga cagattccga tcgtgccgtt cgatctcaat
     8881 cgcgacggca agtcgtggcc cgatccggcg ttcggctccg gcgccgtgaa gatcacgccg
     8941 gcacatgatc cgaacgacta cgacgcgtcg cagatcgcga atctgccggt cctccaggtg
     9001 atcgatccga aaggacgcat gaccgcgccg gcgccggcga agtacgtcgg gatgtctgtc
     9061 gacgacgcgc gcaaagccgt ggtcgcggat ctcgaggcgg gcggattcct cggcgacgtc
     9121 aagccgtaca aagtgccgcg cggccgcagc cagcgctcgg gcgcggtgat cgagccgatg
     9181 ctcatggatc agtggtgggt gaaagccgcg ccgctcgccg agaagtgcgt cgccgccgtc
     9241 gaagcgggca agacgaagtt cgtccccgag ctgtggacga agacgtacat gcactggatg
     9301 acgaacatca aagactggtg catctcgcgc cagctctggt ggggccaccg cattccagcg
     9361 ttctactgcc agaccgagag ctgcggtcac acgaccgtgt cgcgcgaaga cgtcaccgcg
     9421 tgcgccaagt gcggcggcgc gacgaagcag gacgaagaca tcctggacac gtggttcagc
     9481 tccgcgctgt ggccgttctc gacgctcggc tggcccgaca agacgcgcga gctgtcgacg
     9541 ttctatccga acaacgtgct cgtcacggga ccggacatca tcttcttctg ggtcgcccgc
     9601 atgatgatga tgggcatcca cttcatgggg aaggtgccgt tccgcaccgt gtacctgaca
     9661 tcgatcgtca ccgatgaaaa cggcgacaag atgtccaaga ccaaaggcaa cgtcatcgat
     9721 ccgctcgacg tcgtgcacgg cgcgacgctg caagtgctcc tcgatcgcgt cgacgtcgag
     9781 aagccgcccg atcccgagaa ggtcaagaac gcggtcaaga agcacttcgc caaaggcatc
     9841 ccggcgatgg gcgccgatgc gctgcgcttc gcgctcgcgg cgctcaacac cggcagctcg
     9901 cgcatccggc tgtcgatcga gcgcgtcgag ggttaccgca acttcatcaa caagctgtgg
     9961 aacgcatcgc gcttcgcgct gatgaacctc gacggctacg acccggagcg cttcgaagcg
    10021 cagctcgcga cgcccgcggg taaagcgcag ctcggcatgc ccgagaagtg gatcctgtcg
    10081 cgcctgcaag cgacgtctgc cgccgtcgat gcttccctcg aagcattccg cttcagcgac
    10141 gcggcgaacg cgatctatca cttcgtgtgg gacgagctct gcgattggta catcgagctc
    10201 gcgaagccgc acctccacaa gcaggacggc cttcatcaag acaacgtgaa ggccgcgcgc
    10261 cgtcacatcg tgcagggcgt gctcgcgacg acgctcgaga cgacgatgcg cctgttccac
    10321 ccgttcgcgc cgttcgtcac cgaagagatc tggcagaagc tgcccaagcc gccgcagctg
    10381 cccggctcgc tgatgatcac cgtgttcccg cgcggcgatc agcagtgggt cgatccaggc
    10441 gccgaggcgg agatgaagct cttgcaggac gtcgtgtcga cggcgcgcat gctgaagcag
    10501 acgtacggca tcgcgcccgc gcaatcgatc gcggtcgagc tgcgcatcac cgccgacgca
    10561 ccgcgcgcga tcatcgagaa gtacaaggag atggtcgaag cgcgcgcgaa ggtcacatcg
    10621 acgatcacgt cggcgtcgag cggcgcgccg agccccggga cggccaaggc gttcatcggc
    10681 tccgacgtgc aggtcctcat gccgctcggc ggtctgatcg atgcgccgac ggagaaggct
    10741 cgcatctcga aggacatcgg caagtgcgag aaagaaatcg gcgcgctcga gaagaagctc
    10801 gcgaatcaag atttcctctc gcgtgcgccc gaagaggtcg tcgccgagat tcgccagcgc
    10861 ctcgaggacg aaaagagccg catgaagctg ctcaaagacg cactcgaaac gctcggtgaa
    10921 gcgaaatgac gagcggtctc tcgcgcgacc gcgcagccct cctcgttgtc gatatccagg
    10981 agcgactcgc tgcggtgatg cagcccgctg tgctcgagca agtcgtgcgc aacaccgtgg
    11041 tgctcatcga gacagccaat cggttgggcc tgccgatcgc cgtgagccag cagtatccga
    11101 agggcctcgg cgcgacggtg ccagcgatcg aagaggcgct ccgcgcggtc acgacgccgg
    11161 cgggttggcc tggcggcgac cgcgtttatc gcttcgacaa gacggtgttc gcggcgacag
    11221 cgagcgaaca gtttgcgggc gccacacggg atcccctcca gcgcgaccaa tggatcgtgg
    11281 ccggcatgga ggcgcacatc tgcgtctacc agacggcgcg aggcctcgtg acgcgcgggc
    11341 ttcaagcgca cgtcgtgagc gacgccgtgt gcagccgcac gaaggcgaac tggaagatcg
    11401 gctgcaagct cgtcgagcgc ctcggcgacg ctcgtggcct ggacgctacg gcgccggttg
    11461 gcggcggtgg tgcggtgctg acgtcgacgg aggtcgtcgt gttcgatctc ctcggtcgcg
    11521 ctggaaccga cgacttcaag gcgctctcgc gcctgatcaa gtaagctgcg cgccgatggc
    11581 tggctcgacc ggagaagtcc tcaagtccct cgcggtgaac gctgtcatcg cgacgagcaa
    11641 gggtattgcc gcggcgatga ccggctctgg cgcgttgctc gccgagacgc tgcactcgtt
    11701 cgccgattgc ggcaatcagc tgctgctcct cgtcggcatc aagcggtcgg cccggccgcc
    11761 gagcccgaag tatccgctcg ggcagggccg cgagatgtac ttctactcat ttgtcgtcgc
    11821 gctcctgctg ttctcgggcg gcggcgtgtt ctcggtctac gagggaattc acaagctgaa
    11881 gcatcccgag aaggtcggca acatcaccgt cggcctcgtg atcctcggcc tgtcgatcct
    11941 cctcgagggc tggtcgacgt ggggcaacct caaggagatg aagaagcggc gcaaggacaa
    12001 gccgctcctc aagtacctcc gcgacagcaa agactccgac ctcatcgtcg tcttcggtga
    12061 gaacagcgcc gccgtgttcg gcctcgtgct cgcgctgctc tcgctcatcg tcgcgaagca
    12121 gacgaacgac ggccgctggg atgccatcgg ctcgatcgcg atcggcgtcg tgctcgtcgg
    12181 tgtcgcgttg ttcctcgcac gcgagatcaa gagcttgctc gtcggtgaag cggcggaccc
    12241 cgagctcgag gcagcgatga agcagctcgc ggtcgacgat ccgaatgtcg acgagatgct
    12301 gcgcttgctc accgtgcagc agggccccgg cgagatcgtc gtcgcggcga agctgcggtt
    12361 tcgtgcgggc ctcgacacga agcagctcgt cgacgcgatc aacggcttcg agcgcgagct
    12421 gaagaagcgc gtgcccgaag tgcggtggag cttcatcgag cccgaccaca ccgactgagc
    12481 gcggctagat gcaggcgcgg tagatgccgt cggccgcagc aacgaacaac acggtgtccg
    12541 tcgtgccgcg cgcgatcgcg aagccgagtg gcgagggcat accttcgagg cggaccggcg
    12601 cgtacggtgc agccgtcgag tcgcgcgtcg tatagacgct gccttgccgg cgcatcgcga
    12661 tcacgatgcc gctgggatcg acgtcgatga gcggctgcgt cgcgttcgga atcgtcgcga
    12721 tggggacggg cgtgcccggc gacgcggtgt ttgcccaact cgacaactgc aggccgccgc
    12781 ccttctcgta gaccgcgatc gcgacgccgt tgcgctgcac gaggctgatg ttcggacatg
    12841 cctccgggaa gccgccgagc gcggtgccgt tcggactgat gtgcgtcgtg aagcaatacg
    12901 cttcgccgac cgacagcttc ggacatgcga gcgcgatatc gccgttgtcg gccgccgcga
    12961 cacagcccgc cgatggacca cccgtgccgc cgccggggcc tgcgctcgag ccgttgaggt
    13021 cgctggtcac gccgtagtgg aagtagccgg ttccgtgcgc ggcatagaca tcgaagttgc
    13081 cggactgcgt gggaaggatc gcgtcgcctt cggcgattgc atcggtgagc gtcgtgccgc
    13141 tggtagcgac cgacggcgat gcgagtggga tggacgccat gcgtgtcttg tcgacgtcga
    13201 gcacgccgat cacgagacga ttgccatccg cgagtccagc gacggagctt ccggcgaggt
    13261 tcacgacgag cgggacctgg tcgacaggtt cgagagcggt cgtgtagcgc tgcaggcgca
    13321 agttgccctg atcgcgctcg atcgcgagca cgacgaaacc ctcggtcgtc ttcaccgccg
    13381 tcatcgagac gtcggatccg aagagggtcg cgccgactct gcgtggcgca catcggctct
    13441 gcatgtcggc cggcgggctg tccggatcgt tcgtcgcgtc gacgagcacg ggctgggaca
    13501 gccccagcga ctgggcgcag cccccgagca gtagcatcac cgcacggcgc acctttccac
    13561 agtagcgcga acgctcggtg gatctgtagc tgagctgact tcgccgagga gatccacgtt
    13621 agacgtacga cccggcgccg cgagattcgc gtagattaac gcatggtgtc gaccgccatc
    13681 gcttctcgtt ccaacgacct gtacgagctc ggtgacgttc cggagctggg cgtggttcca
    13741 gcccggatgc tcgcccaggt catccgcgcc gagcgtttcg gcgagccccg tgacgccttc
    13801 aaagaggagc cgctgcccat ccccgagctc ggtccgcgtg acgtgctcgt gtacgtgatg
    13861 gctgcgggcg tcaactacaa caacgtgtgg gccgcgatgg gcatcccgat cgatgtcatc
    13921 aaaggccgcc agaagcgctg gggcgcgaaa gaggacttcc acatcggcgg ctcggatgcg
    13981 tcgggcgtcg tgtgggccgt cggcaaagac gtcaagaacg tcaaggtcgg cgatgacgtc
    14041 gtcgttcact gcggcatgtg ggagcccgac gatccgtggg ttctcgccgg caaggatccg
    14101 atgttcgcgc cgacccagca gatctggggc tacgagtcga actggggctc cttcgcgcaa
    14161 tacacgaagg tgcaggacca ccagtgcctg ccgaagccgc cgcacctgac gtgggaagac
    14221 gcggcggcgt acatgctcgt cggcgcgacc gcataccgca tgctgcacgg ctggtcgccg
    14281 aacgtgatcg gacccggcga cccggtcctc atctggggcg gtgccggtgg cctcggctcg
    14341 atggcgatcc agatctgcaa agccgctggc gcgcgtcccg tcgcggtcgt ctcgggcgac
    14401 agcaagaacg agttctgcaa gaagctcggc gccgtcggcg tgatcgatcg ccgcaagttc
    14461 gatcactggg gcaagctgcc gcgctggtcg gacgacatcg agtacgcgca gtggctcaag
    14521 ggcgcgcgcg cattcggtgc tgcgttctgg gaagcgctcg gcgagaagaa gaatccgacg
    14581 atcgtgttcg agcatccagg cgagacgacg attccgacgt cctgcttcat ggtcgagacg
    14641 ggcggcatgg tcgtcatctg cgcgggcacg accggctaca acgcgacgct cgatcttcgt
    14701 taccactgga tgcgccagaa gcgcctgcaa ggcagccact tcgcgaacga cgaccagtgc
    14761 aagggcttca acgatctcgt cctcgcgggc aagatcgacc cctgcatgtc gcgcgtgttc
    14821 aagttcgacg agaccggcga gtgccaccag ctgatgcgcg agaaccagca tccgtcgggc
    14881 aacatgtcga tcctcgtcaa cgcgccgcgc accggcctgc gtacgctcga cgacgttcgc
    14941 aacgcgaagc gctgatgtcg acgcggccgt ttcgagtgct gggcctccag cagatcgcga
    15001 tcggcggtcc gagcaaggac gcgttgcgcc gcctctggat cgatctgctc ggcgttccgc
    15061 agacaggcac gttcaagagc gagcgtgaaa acgtcgacga ggacatcctc gcggtcgccg
    15121 gcgtcgagat cgatctgatg cagccgatcg atccagagaa gaagcccaaa gtgcacgagc
    15181 ctcggctcaa tcacatcggg ctgtggatcg acgacctccc ggccgcggtg acgtggctcg
    15241 aagggcaggg cctcacgttc acgccgggcg gcatccgcaa gggcgcagca ggccacgacg
    15301 tctgcttcat tcacccgaag tctgccgaag gcgtgctgat cgagctcgtg caagcgccgc
    15361 ccgacgtcat cgccgctgcg aagtagctca gagaatgatc gcggccgcgg cgagacggaa
    15421 gatcaagaac gccttcgttc ggttcacgcc cgtcgagtga aacgcgtcgc tgctcgaggc
    15481 gtcgctcggg tcgtaccaca tccaatgcgc ggcgctgtcg atgccggggc tcgcggtctg
    15541 gccgggctgg cagacgatcg gaaacgttcc agcggctgcg tcgttcgctt caccgacagc
    15601 aagccggccg agcgcgctcg gcgtccctgc agaggctccg gtcgtgctca cccagccttt
    15661 gctcgtgttg gtgtcgcctg acgcggcatt gcacttggca atctcggcgt tcacgatcga
    15721 ctgcgccggt cccgtcgtcg cgttcgcgcc gcccgagtaa tcgaggcccg tcgcgcagac
    15781 ctcgaagcga tcgtcgccgg tgagcacggt gccggtgtcg cgcttgaatt gtgcgaggac
    15841 accctgcgtg accgcgaggt catcccacgt cacgacgtag aggtaggcgc cgtcgggcgc
    15901 atccgcggcc gcgaccgtgt attgctcggg accttcgccg cagttccaga tctgaccggc
    15961 cgtctgcgcg cgctggcctt ggatgaacgt cgtgatggtg tcggcggttc cgtagccgaa
    16021 gctgtaggcg ttgtcggccg tgatgacgac atcgacgggc cccgaggctt gcggcggggg
    16081 cgtatcgacg acggtcgccg aatcgggcga cgtggccgcg tccggttggc cggccggctc
    16141 ccgcgtcgta ggtccgcacg cgcacacgaa gatcgccacg ataccgacac accggttcat
    16201 gccgcgacgc tagaaggatt ggccgagggc cgcgacaaat agattcggtc acgccccggg
    16261 gccggattac ctggcgtacg atcgatcgat ggggcgctgg ctgctcgtgc tcgccgtcgt
    16321 cggtgcatgc aatcaggcat tcgatctcga gccgacgctc ggtccggaca gcgatggtga
    16381 cgggattgcc gacggggatg acaactgcct gctcgtcgga aaccacgacc aggccaacga
    16441 ggaccacgac gcgctcggcg atgcgtgcga tccgtgcgtc gcggggagcc agctcggcat
    16501 cgacggcgac cgcgacggcg ttgacgacgc gtgcgacgcg tgcctcgagg gacgcaacag
    16561 cgacgaggac gacgatggtg ttctcgacgg gtgcgatgac tgcccggccg acgcggacgc
    16621 ggaccagctc gacagtgacg gcgacggagt cggcgatgcg tgcgacatcg ggccggcggt
    16681 gcagaacacg cgcttgctgt tcgacggctt cgcgccgccg gtgccgcatt gggacacggg
    16741 gttctgggaa tggcgcgccg ttgacggttc cttcggaccg gtcactccca tcaggagcaa
    16801 gtacgacggc gcgtggaacc ctgacatcgt cgtgccgggg cgacgctggt atctcgatac
    16861 gtcgatcggg attttcgccg acagcgcatt cgacggcgcg tatatcaagc tcgtggcgct
    16921 gacgtcactc ggtgggttca cggctcgcga ctgcggcatc caattcaacc agggccagtg
    16981 gcgggatccg gtcacgtcga cggtgatcgt ggtgggcgcc gcaacgcgcc ttcggctcgt
    17041 cgataccggt gcggttgtgc agtgctggat caacggcacg ctcatgcaca caaaaccgag
    17101 caccgacccg accacggctt ggctgccgtt cctgcaaacc aacacgcaat cggagtttcg
    17161 gtggctcgac gtcgtcccgt gatcgccggc ggtgcgctcg tgctcgcgtt gtccgcatgc
    17221 aacgcggcgt tcgggctcgt gcccacgctc ggcccagaca gcgacggtga tggtcacgac
    17281 gacggccatg acaattgtcc cctcgtcgta aatcccgacc aagcggatcg cgacgacgac
    17341 acgctcggcg acctctgcga tccgtgcgat ggcccgcaag ccggcaccga caatgatcac
    17401 gacggcgtcg acgacggttg cgatgcctgc ctcaccggtc gcaacaccga cgaagatcac
    17461 gacgcgtatc tcgacgggtg cgatgcgtgc cccggcacgt cggacgatca agcggatgat
    17521 gacggcgatg gcatcggcaa cgcatgcgac gcagatcccg gtgcgcagaa ccatcgcgtg
    17581 ttcttcgacg gattcgatcc gccgctcagc gattggcgtg catggctcac gacctggcac
    17641 ccgacggaag gtggcggctt cgagccggtc ggtatcagtc ccggcgggac cggtgcgtgg
    17701 catccagcct ccgtggtgac gggcaacgaa ttctgggccg aggcggtgat cgacgttccc
    17761 a
//
LOCUS       DATLBZ010000168         9488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2397360, whole genome shotgun sequence.
ACCESSION   DATLBZ010000168 DATLBZ010000000
VERSION     DATLBZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397360"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VMZ53_08890"
     CDS             <1..1155
                     /locus_tag="VMZ53_08890"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS28613.1"
                     /translation="RVYAEKAALIAGKRGARLLRAKALLKQGWALWTQGRDDDVAPIY
                     EEAKQLFSELGDRSGLARTLNNIGLAQHRQHRDTEAGRSYADGLRIAEEIGDTEAQAW
                     LLNNWAYLYFDAGDPGKALELHMKKLKLGGERGDLPSSQAAGHINVSEILRLRGDLAG
                     ARDHCNQAEDLLRGLDARRFAAFTAGYCGELLRVSDDLVGAKKKFEQALGWASDVMTP
                     AESAEMRVAMARAELDDNHAAEAEALTRTALADLAAVNEGSQRVCASAILATALLTRD
                     RVADAAAEIETTSKLPAEGVSFPCRIEAEIARAHVDIARDPSSKADVLARLEKTRAAA
                     AAATFVQWELAARLAIGELSGDRAALKKLAKDADTLGFKLVARRAKALAR"
     gene            complement(1156..1950)
                     /locus_tag="VMZ53_08895"
     CDS             complement(1156..1950)
                     /locus_tag="VMZ53_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28614.1"
                     /translation="MKKFGLLMTVLAACGGDDGVQMMVDAKPMTVEHDLSCLNYTPPS
                     VPMTIRLDGIILDFTTVPSPPTVPDTTLHVTSLDGATEITSATSDMDGKLTLTIPTTG
                     VPTRFRYAIDATGFPKSRTFVSVGPAKDAIDPMRAAFPVVQQSRIDAIATALGETLDP
                     TKGTLEVVMFDCSFGDYYAATPSIVEEPGLKWAMNGGANAWLPRATTLHHPNNRSESV
                     AAAVNLTPGPKTFMLSDATTTIGPISFTIEADTYTTLIVQPGYPRQ"
     gene            2023..3012
                     /locus_tag="VMZ53_08900"
     CDS             2023..3012
                     /locus_tag="VMZ53_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUS28615.1"
                     /translation="MKLATLRDGSRDGRLLVIRRDGEMGAPSPEKWPTLQSALDDWNA
                     TEPHLRKIADQLDRGALDGMAVDPHRLGAPLPRAYEWVDGSAFLNHVILVRKARNAEP
                     PKTLETDPLIYQGGSGDMLGPRDPIELHDPAWGLDFESEVGVILGDTPIGTTAADAQH
                     YVRLIVLLNDCTLRNLIPDELAKGFGFFQSKPATAFAPFAVTPDELGDAWRGGRAHVR
                     VRSTYNENQVGDCEAGGEMHFSFFDLIQHVTRTRRYTAGTILGSGTVSNADRERGISC
                     LAERRMIETIEGGKPTTPFMSVGDHIEIEAFDQAGASPFGKIDQRVVAAGSPK"
     gene            3009..3653
                     /gene="maiA"
                     /locus_tag="VMZ53_08905"
     CDS             3009..3653
                     /gene="maiA"
                     /locus_tag="VMZ53_08905"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HUS28616.1"
                     /translation="MKLVLHNYWRSSASHRVRIGLGLKKLKYDYVVVNIVKSEQYADA
                     YRSRNPMGQVPTLEITEDDGSVRAISQSLPILEYLDERFAEPAILPKDLYMRAKARAL
                     AEIVNSGIQPHQNLTTTKAVKAMGGDEVVWPKGFISDGLIAFERVAQETAGKFCVGDA
                     PSIADCCLIPQLASARRFGVDVTKQSLLLGIEERCLALPAFADAAPDKQPDAVK"
     gene            3657..4802
                     /locus_tag="VMZ53_08910"
     CDS             3657..4802
                     /locus_tag="VMZ53_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS28617.1"
                     /translation="MAKLESLGIKRLEGIHYYVRDLERSRQFYCGKLDFTETWRSAPD
                     LEQTGKQKSACFSAGNINVVCSSPVGEGGRAARFLSKHPDGVGTLSFEVEDIAKTFRL
                     LEERGGTPIDDIQTFTDDGGTLKQFSITTPFGDCTFRFREHRGYKGLFPGAVPHQAGG
                     KNKFGFQEIDHVTSNFQTMKPMLLWLEHVMGFEQLWEVEFHTSDVDPNRKTGSGLKSI
                     VMWDPISNTKFANNEPARPFFKSSQINIFNEELRGDGVQHVALTVADIVPAVRALRAN
                     GVTFMPTPGSYYEMLPERIAKTGIKQIDEDINILRDLEILVDGDHFHAYMLQIFLKES
                     AGLYGDPNAGPFFYEIIQRKGDRGFGAGNFRALFESIEREQRERGIV"
     gene            4802..5959
                     /locus_tag="VMZ53_08915"
     CDS             4802..5959
                     /locus_tag="VMZ53_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HUS28618.1"
                     /translation="MLDRMQVGDVARKHHIQLRGEDGALRFEECFTRDGFDGPYTIMY
                     HLRRPHVQRVAPAKHGWTAPVAASERHLAKRHYKTWELAAQARGPQLDTRIALHFNAD
                     VIAGVAFPTAEDPVYSIDGDADQLIYIHKGGGTLRSQLGDVAFAQGDYVFVPRGLIHR
                     FMPASTEQHWFWFSLMGGLHIPKQWRNEVGQLRMDAPYSERDFKRPQLRAPFDENIRE
                     LVVRREGKWHGFTIDDSPFDVVGWDGAVYPWAFPILAFQPRVSSVHLPPTWHGTFAAR
                     GALVCSFVPRLVDFHPEAIPCPYPHSSTHCDEIIFYCEGNFTSRKGVSAGSISHHPMG
                     IPHGPHPGAYEKSIGTTRTDEIAVMCDTFQTLQVTTAALAVEDPGYHDSFL"
     gene            complement(5956..6420)
                     /locus_tag="VMZ53_08920"
     CDS             complement(5956..6420)
                     /locus_tag="VMZ53_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28619.1"
                     /translation="MRLVVLAVVLVAVFASPARADDGDLLNYSIGPVFGIKLSGPGDW
                     PVIGIEGGVGYGPERINVGFEHRSDHDVGYIELDPWYLIGASIGFTVNEKNETTGLFG
                     LWEGLPLKGMDRGSCYGWRNMVTIAGGYRYTGVHELFVTVKGGRIDGQLCFD"
     gene            complement(6439..6987)
                     /locus_tag="VMZ53_08925"
     CDS             complement(6439..6987)
                     /locus_tag="VMZ53_08925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HUS28620.1"
                     /translation="MTAVSLLGICGSTRKASLNRALLVAVAEVLPEGTAFSMYEGLDQ
                     LPIFNSDLQEPTSVLALKSAIAAADGLVIAVPEYNYSIPGGLKNALDWVSRPPATSPM
                     RGKPVGLVGAATGMSGTIRAQTHMRQMLVYSDTPVLNQPEVLIPRAQERFTDGELTDE
                     STRQLLGRFATAFIAFVRRYKA"
     gene            7012..7761
                     /locus_tag="VMZ53_08930"
     CDS             7012..7761
                     /locus_tag="VMZ53_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS28621.1"
                     /translation="MRLVVALLLCAVTGCGRLSFDARSDAARDDSVADALDAPPGLIA
                     WLPLDTLTTTTPDISGHAHHATCAGASCPLSIAGHRGGGMRFDGVDDELRIAGTSDLQ
                     TSAGFTIVVWLRVAAFPTTMRACLAQKMLGTMTFDSWELCVETDGRLFFYSASDTVDL
                     SITSVGPYQLDRWHQITIVWDGNMKRMYEDDAILAGSSNQMISFDGNDVVLGRDYDGG
                     SPLSEFAGDLDDFQIYDRALSVPEITQLASQ"
     gene            7793..8443
                     /gene="msrA"
                     /locus_tag="VMZ53_08935"
     CDS             7793..8443
                     /gene="msrA"
                     /locus_tag="VMZ53_08935"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HUS28622.1"
                     /translation="MFFSSKPLRMPSPEDALRGRTSKMPVEGTHFVNGNRIAPPFPDG
                     MKMAMFGMGCFWGAERKFWQTSGVYATAVGYAAGYTPNPTYEEVCSGMTGHNEVVLVI
                     YDPAQVTFEKLLQVFWESHDPTQGMRQGNDRGTQYRSGIYTFDEEQAKAAQASKAMYQ
                     QQLSAAGHDEITTEVKPAGEFYYAEDYHQQYLGKNPGGYCGLGGTGVSCPIGVGAR"
     gene            complement(8440..9336)
                     /locus_tag="VMZ53_08940"
     CDS             complement(8440..9336)
                     /locus_tag="VMZ53_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28623.1"
                     /translation="MRIVLLSLLALAACGRVGFDETASGDATSGDSTMPDAFEVLVLD
                     PPFGTPMLVSEISSASFEDDPTLSGDQLEVMFTSARPGGLGGNDLWASSRASVSDPWP
                     TPHDVVELSTAANDTNPELSRDGLSLVYSSGTAGDQDLYFVTRASRAAPWGVATRLTA
                     VSSGVNDFGGAMSDDLAAVYFASARGGSLDIFAARGSAGAWSSPVEITELSTAGVDTS
                     PFVMLDETVMWFASDRPGTAGQHDIWVAIRPSPGAAFDPPLRIGQLATANDDSDPWVS
                     PDGHVIYFSRTTTGTDLYMATR"
BASE COUNT         1728 a         2934 c         3147 g         1679 t
ORIGIN      
        1 cgtgtgtatg ccgagaaggc cgccctgatc gccggcaagc gaggagcccg gctgttgcgc
       61 gcgaaggcgc tcctcaagca aggctgggca ctgtggacgc aaggccgcga tgatgatgtc
      121 gcgccgatct atgaagaggc gaagcaatta ttcagcgagc tcggcgatcg ttccggactc
      181 gcgcgaacgc tgaacaacat cggccttgcg cagcatcgac agcaccgcga caccgaggcc
      241 ggacgctcat atgccgacgg ccttcgcatc gccgaggaaa tcggtgacac cgaagcgcag
      301 gcgtggctgc tcaacaattg ggcgtacctc tatttcgacg ccggagatcc gggcaaggca
      361 ctagagctgc acatgaagaa gttgaagctc ggtggtgagc gcggtgatct gccgagcagc
      421 caagcagccg gacatatcaa cgtctcggag attttgcgcc tgcgcggcga tcttgccggc
      481 gcacgcgatc actgcaacca ggccgaagat ctgttgcgtg gcctcgatgc ccgacggttt
      541 gcagcattca cggcgggcta ttgcggagag ctcttgcgcg ttagtgacga tctcgtaggt
      601 gcgaaaaaga agttcgaaca agcgctcggg tgggcgtctg atgtcatgac gcctgccgag
      661 tcggctgaga tgcgcgtcgc gatggcgcgt gcagagctcg acgacaacca cgctgccgaa
      721 gcggaggcgc tcacgcgcac ggcgttggca gatctcgcag cagtgaacga ggggagccag
      781 cgcgtctgcg cgagtgcgat tcttgcgacc gcgctactaa cgcgcgacag agttgcggac
      841 gcagcagctg agatagagac gacgtcgaag ctgccggcgg aaggcgtcag cttcccgtgt
      901 cgaatcgagg ccgaaatcgc gcgagcgcac gtcgacattg cgcgcgatcc gtcgagtaag
      961 gcagatgtgt tggcgcgcct cgagaaaaca cgagcagcag ctgctgcggc gacgttcgtc
     1021 cagtgggagc tcgctgcccg cctcgccatc ggcgagctct ccggggatcg tgctgcgctg
     1081 aagaagctcg cgaaggatgc cgacacactc ggcttcaagc tcgttgcgcg gcgtgcgaaa
     1141 gcactcgctc gctgatcact gacgcggata accgggttgc acgatcagcg tggtgtacgt
     1201 atccgcctcg atcgtgaatg agattgggcc gatcgtcgtg gtggcgtcgc tgagcatgaa
     1261 cgtcttcgga cccggcgtca ggttcaccgc agctgccacg ctctcgctgc gattgttcgg
     1321 gtgatgcagc gtggtcgcgc gtgggagcca tgcgttggcg ccgccgttca tcgcccactt
     1381 cagacccggt tcttcgacga tgctcggcgt ggcggcgtag tagtcgccga agctgcagtc
     1441 gaacatgacg acctcgagag tgcctttcgt tggatcgagc gtctcgccga gtgcggtggc
     1501 gatggcatcg atgcgcgatt gctgcacgac cgggaacgcg gcacgcatcg gatcgatggc
     1561 gtccttcgcc ggaccgaccg atacgaacgt gcggctcttt ggaaagccgg tcgcgtcgat
     1621 cgcgtagcga aaccgagtcg gcacgccagt ggtcgggatg gttagcgtga gcttgccgtc
     1681 catatcgctc gtcgcgctcg tgatctcggt cgccccgtcg agcgaggtga cgtggagtgt
     1741 cgtgtccgga accgttggcg ggctcggcac cgtcgtgaaa tcgaggatga tgccgtcgag
     1801 cctgatcgtc atcggtactg atggaggcgt gtagttgagg cacgagaggt cgtgctcgac
     1861 cgtcatcggt tttgcgtcga ccatcatctg aacaccgtcg tcgccgccgc acgcggcaag
     1921 caccgtcatc aggagaccga atttcttcat agtgggcgcc aacctacacg gttttcgcag
     1981 accttttcca ggggcggctc gtgggataag gtgccgtcgg cgatgaagct tgcaactctg
     2041 cgcgatggct cgagagatgg ccggttgctc gtcattcggc gggatggcga gatgggcgca
     2101 ccgtcgccgg agaaatggcc gacgctgcag agcgcgctcg acgattggaa cgcgacggag
     2161 ccgcatctgc gcaagatcgc cgaccagctc gatcggggag cgctcgatgg catggcggtc
     2221 gatccgcatc ggctcggtgc accgctgccg cgcgcgtatg aatgggtcga tggctcggcg
     2281 tttttgaatc acgtgattct ggtgcgcaag gcgcgcaacg cggagccgcc gaagacgctc
     2341 gagaccgatc cactcattta tcagggcggc tcgggcgaca tgctcggacc tcgcgatccg
     2401 atcgagctgc atgatcctgc gtggggcttg gacttcgagt cggaggttgg cgtcattctc
     2461 ggcgacacgc cgatcgggac gaccgccgcg gatgcgcagc attacgtgcg gctcatcgtc
     2521 ctcttgaatg actgcacgct gcgcaacctg attcccgacg agctcgcgaa ggggttcggc
     2581 ttctttcaga gcaagccggc gaccgcgttc gcgccgtttg cggtcacgcc cgacgagctc
     2641 ggcgatgcgt ggcgcggtgg gcgggcacac gtgcgcgtgc ggtcgacgta caacgagaat
     2701 caggtcggcg actgcgaggc cggcggcgag atgcacttct cgttcttcga tctcatccag
     2761 cacgtcacga ggacgcgccg gtatacggcc ggaacgattc ttggctcggg caccgtttcg
     2821 aacgcggatc gcgagcgcgg gatttcgtgt ctcgcggagc gccggatgat cgagacgatc
     2881 gagggcggga agccgaccac gccgttcatg tccgtcggtg atcacatcga gatcgaagcg
     2941 ttcgatcagg cgggcgcgag cccattcggg aagatcgatc aacgcgtcgt agctgcgggg
     3001 agtccgaaat gaagctcgtg ctgcataact attggcgttc gtcggcgagc catcgcgttc
     3061 gcatcgggct tgggctgaag aagctgaagt acgactacgt cgtcgtgaac atcgtgaaga
     3121 gcgagcagta cgcagatgcg tatcgatcgc gcaatccgat ggggcaggtg ccgacactcg
     3181 agattaccga ggacgacggc tccgttcgcg cgatttcgca gtcgctgccg atcctcgagt
     3241 atctcgacga gcggttcgcg gagccggcga tcctgccgaa ggatctgtac atgcgagcga
     3301 aggcgcgtgc gctcgccgag atcgtgaact ccggcattca gccgcatcag aacctgacga
     3361 cgacgaaggc cgtgaaggcg atgggcggcg atgaggtcgt gtggccgaag ggcttcatct
     3421 cggatggtct catcgcattc gagcgcgtcg cgcaggagac ggccggcaag ttctgcgtcg
     3481 gtgatgcgcc gtcgatcgcg gactgctgct tgattccgca gctcgcgtcg gcgcggcggt
     3541 tcggcgtcga cgttacgaag caatctcttc tgctcggcat cgaggagcgc tgtctcgcgc
     3601 ttcctgcatt tgcggacgca gctcctgaca agcaacccga cgcggtgaag tgatctatgg
     3661 cgaagctcga atctctcggc atcaagcggc tcgaaggcat tcactactac gtgcgtgacc
     3721 tcgagcggtc gcggcagttc tactgcggca agctcgactt caccgagacg tggcgcagcg
     3781 cgccggatct cgagcagacc ggcaagcaga agtcggcgtg cttctcggca ggcaacatca
     3841 atgtggtttg ttcgtcgccg gtgggcgagg gcggtcgcgc agcgcgcttc ctctcgaagc
     3901 atcccgacgg tgtcggcacg ctcagcttcg aggtcgagga catcgcgaag acgttccggc
     3961 tcctcgagga gcgcggtggc acgccgatcg atgacatcca gacgttcacc gacgacggcg
     4021 ggacgctgaa gcagttctcg atcacgacgc cgttcggcga ttgcacgttc cggttccgcg
     4081 agcatcgcgg ctacaagggg ctgttccccg gcgcggttcc gcaccaggcc ggcggcaaga
     4141 acaagttcgg ctttcaggag atcgatcacg tcacgtcgaa cttccagacg atgaagccga
     4201 tgctgctgtg gctcgagcac gtgatgggct tcgagcagct ctgggaggtc gagttccaca
     4261 cgagcgacgt cgatccgaat cgcaagaccg gctcgggcct caagtcgatc gtgatgtggg
     4321 atccgatctc gaacacgaag ttcgcgaaca acgagcccgc gcggccgttc ttcaagagct
     4381 cgcagatcaa catcttcaat gaagagctcc gcggcgatgg cgtgcagcat gtcgcgttga
     4441 cggtggcaga catcgtccct gcggttcgcg cgctgcgcgc caacggcgtg acgttcatgc
     4501 cgacgccggg cagctactac gagatgttgc ccgagcggat cgcgaagacg ggcatcaagc
     4561 agatcgacga ggacatcaac atcctgcgcg atctcgagat cctcgtcgac ggcgatcact
     4621 tccacgcgta catgctgcag atcttcctca aggagtcggc aggcctctac ggcgatccga
     4681 acgcgggccc gttcttctac gagatcatcc agcgcaaggg cgaccgcggc ttcggcgcgg
     4741 gcaacttccg cgcgctgttc gagagcatcg agcgcgagca acgcgaacga ggaatcgtct
     4801 aatgctcgac cgcatgcagg tcggcgacgt cgcgcgcaag catcacatcc agctgcgcgg
     4861 agaggacggc gcgctgcgct tcgaagaatg cttcacgcgc gacggcttcg acggtccata
     4921 cacgatcatg tatcacctgc gccggccgca cgttcagcgt gtcgcgcctg cgaagcacgg
     4981 ttggacggcg ccggttgcgg catcggagcg ccacctcgcg aagcgtcact acaagacatg
     5041 ggagctcgct gcgcaggcga ggggaccgca gctcgacacg cgcatcgcgc tgcacttcaa
     5101 cgccgacgtg atcgcgggcg tcgcgtttcc cacagccgaa gatccggtct actcgatcga
     5161 tggcgatgcg gatcaactga tctacatcca caagggcggc ggcacgctgc gctcgcagct
     5221 cggcgacgtc gcgttcgcgc agggcgacta cgtcttcgtg ccacgcggcc tcatccatcg
     5281 cttcatgccc gccagcaccg agcagcattg gttctggttt tcgctgatgg gcggcctgca
     5341 catcccgaag caatggcgca acgaagtcgg ccagctccgc atggatgcgc cgtacagcga
     5401 gcgcgacttc aagcggccgc agctgcgcgc gccgttcgac gaaaacattc gcgagctcgt
     5461 cgtgcgtcgc gagggcaaat ggcacggctt cacgatcgac gactcgcctt tcgacgtcgt
     5521 cggctgggat ggcgcggtct atccgtgggc gttcccgatc ctcgcgttcc aacctcgcgt
     5581 gtcgagcgtc catctgccgc cgacgtggca cggcacgttc gctgcgcgcg gcgcgctggt
     5641 ctgtagcttc gtgcctcggc tcgtcgactt ccaccccgaa gcgattccgt gcccgtatcc
     5701 gcactcgagc acgcactgcg acgagatcat cttctactgc gaaggcaact tcacgtcgcg
     5761 caagggcgtg tccgccggca gcatctcgca tcacccgatg gggatcccgc acggcccgca
     5821 tccgggcgcg tacgagaagt cgatcggaac aacgcgcacc gacgagatcg cggtcatgtg
     5881 cgacacgttc cagacgctac aggtcacgac cgcggcgctc gccgtcgaag accccggcta
     5941 tcacgactcg ttcctctaat cgaaacagag ctggccgtcg atgcggccgc ccttcaccgt
     6001 cacgaacagc tcgtgcacgc cggtgtaccg gtagccgccg gcgatggtca ccatgttgcg
     6061 ccagccgtag cacgagccgc gatccatccc tttgagcggc aggccttccc acaggccgaa
     6121 caagccggtc gtctcgttct tctcgttgac ggtgaagcca atcgaggcgc cgatcagata
     6181 ccaaggatcg agctcgatgt agccgacgtc gtgatcgctg cggtgctcga agccgacgtt
     6241 gatgcgctca ggtccgtaac cgacgccgcc ctcgatgccg atgacgggcc aatcgcccgg
     6301 accactcagc ttgatgccga agacgggacc gatgctgtag ttcagcaggt cgccgtcgtc
     6361 ggcgcgcgcc ggtgacgcga agaccgcgac gaggaccacc gccaacacga cgagtcgcat
     6421 cggctcagcg tatcgcgctt acgccttgta gcgacgaaca aacgcgatga atgctgtcgc
     6481 gaaccggccg agcagctgcc gcgtcgactc gtcggtgagc tcgccgtcgg tgaatcgctc
     6541 ctgcgctcgc ggaatgagca cttccggctg attgagcacg ggcgtgtcgc tgtagacgag
     6601 catctggcgc atgtgagttt gcgcgcgaat ggtgccgctc atgccggtcg ctgcaccgac
     6661 gagaccgact ggcttgccgc gcatcggcga tgttgcgggc gggcgcgaga cccaatcgag
     6721 cgcattcttc aggccgccgg ggatgctgta gttgtactcg gggactgcga tcacgaggcc
     6781 gtccgctgca gcgatcgcgg acttcagggc aaggacgctc gtcggctctt ggagatcgct
     6841 gttgaagatc ggcagctgat cgaggccttc gtacatcgag aacgccgtgc cctcgggcag
     6901 cacctcggcg accgcgacga gcaacgcgcg attgagcgat gccttgcgcg tgctgccgca
     6961 gatgccgagg agagacaccg ctgtcatgcg cggcaaggta gcatgaggaa agtgcgtctc
     7021 gtcgttgcgc tcttgctttg cgccgtgacc ggatgcgggc gtctgtcgtt cgatgcgagg
     7081 agcgatgcgg cgcgcgacga tagtgtggcg gatgcgctcg atgcaccgcc ggggctgatc
     7141 gcgtggttgc cgctggacac gctcacgacg acgacgcccg atatcagcgg tcatgcgcat
     7201 cacgcgacat gcgccggtgc gtcgtgcccg ctctcgatcg ccggtcaccg tggcggcggc
     7261 atgcgattcg atggcgtcga tgacgagctg cggattgcag gcacgtccga tttgcagacg
     7321 tcggccggtt ttacgatcgt cgtctggctt cgggtcgccg catttccgac gacaatgcgc
     7381 gcgtgtctcg cgcagaagat gctgggcacg atgacgttcg actcgtggga gctatgtgta
     7441 gagacggacg gcaggctgtt cttctactcg gcgagcgaca cggtggattt atcgatcacg
     7501 tccgttggcc cctatcagct cgaccggtgg catcaaatca ccatcgtgtg ggacggcaac
     7561 atgaagcgta tgtatgagga cgacgcgatc ctcgcgggtt cgtcgaacca gatgatttcg
     7621 ttcgatggca acgatgtcgt gctcggccgc gattatgacg gtggctcgcc actcagcgaa
     7681 tttgcagggg acctcgacga cttccagatc tacgaccgcg cgctctcggt ccccgaaatc
     7741 acccagctag ctagccagta accaacggct gagcgcgccg tatactgcgc gtatgttctt
     7801 ctccagcaag ccgctgcgca tgccgtcgcc cgaggacgcg ctgcgcggac gcacgagcaa
     7861 gatgcccgtc gagggcacgc acttcgtcaa cggcaaccgc atcgctccgc cgtttcccga
     7921 cggcatgaag atggcgatgt tcggcatggg ctgcttctgg ggcgccgaac gcaagttctg
     7981 gcagacgtcc ggcgtctacg cgaccgcggt cggctacgca gccggctaca cgccgaaccc
     8041 gacgtacgaa gaggtctgct ccggcatgac cggccacaac gaggtcgtgc tcgtcatcta
     8101 cgatccggcg caggtcacgt tcgagaagct tctccaagtc ttctgggaga gccacgatcc
     8161 gacgcagggc atgcggcaag gcaacgatcg cggcacgcaa tatcgctcgg gcatctacac
     8221 gttcgacgag gagcaggcga aggccgcgca agcgtcgaag gcgatgtatc agcagcagct
     8281 gagcgcggcg gggcacgacg agatcacgac ggaagtgaag cccgcaggcg agttctatta
     8341 cgccgaggat tatcaccagc agtatctcgg caagaatccc ggcggctact gcggcctcgg
     8401 tggtaccggc gtcagctgtc cgatcggcgt cggcgcgcgc tagcgcgtag ccatatacaa
     8461 atcggtgccc gtcgtcgtac gcgagaagta gatgacgtgt ccgtcgggcg agacccacgg
     8521 atcgctgtcg tcgtttgcgg tcgcgagctg accgattctt agcggcggat cgaatgcagc
     8581 gcctggcgac ggacggatcg cgacccagat gtcgtgctgg cctgcggtgc cggggcgatc
     8641 cgacgcgaac cacatgacgg tctcgtcgag catgacgaac gggctcgtgt cgacgccggc
     8701 cgtcgacagc tcggtgatct cgacgggaga gctccacgcg cctgcggaac cgcgcgcggc
     8761 gaagatgtcg agactgccgc cacgcgccga cgcgaagtaa accgcagcga ggtcgtcgct
     8821 catcgctccg ccgaaatcgt tgacgccgct gctgacggcg gtgagtcgcg tcgcgacgcc
     8881 ccacggagca gcgcgcgatg cacgcgtgac gaagtacaga tcttgatcgc cggccgtccc
     8941 actcgagtag accaacgaca ggccgtcgcg agacagctcc gggttcgtgt cgttcgcggc
     9001 ggtgctcagc tcgacgacgt cgtgcggcgt aggccacggg tcactgacgc tcgcgcgaga
     9061 tgacgcccac aagtcgttgc cgcccaagcc tccgggacgc gcggacgtga acatgacctc
     9121 gagctgatcg ccgcttagcg tcggatcgtc ctcgaacgat gcgctgctga tctcgctgac
     9181 gagcatcggc gtgccgaacg gcggatcgag cacgagtact tcgaaggcgt cgggcattgt
     9241 cgagtcgccg ctcgtcgcat cgccgctagc cgtctcgtcg aagccaacac ggccgcatgc
     9301 ggctagcgcg agcaagctca acaacacgat ccgcacgcgt ctaaaggatc gcgcacgccg
     9361 acgggaactg ccagttacgt ccgcgattcg ttgcgagcga cgagtgcgag aatgtcgtcg
     9421 ccgaagcgag cgatcttcga cgggccgagg cccggaatcg cgaggagctc ttcatgcgtg
     9481 cgcggccg
//
LOCUS       DATLBZ010000169         8811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6044136, whole genome shotgun sequence.
ACCESSION   DATLBZ010000169 DATLBZ010000000
VERSION     DATLBZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6044136"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VMZ53_08945"
     CDS             <1..249
                     /locus_tag="VMZ53_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28624.1"
                     /translation="RGEGADVATIRTGNLDRLTIPDNGAYTMAPNSLKAEKDKTRENT
                     IELRRTNRVTPAGALGGSSFQVSIENRVDVIALANPAL"
     gene            complement(246..3656)
                     /locus_tag="VMZ53_08950"
     CDS             complement(246..3656)
                     /locus_tag="VMZ53_08950"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28625.1"
                     /translation="MAGSDDKLTVALDETLTDVDRSRTVQVRPRTLGLPTIDPSLYDL
                     GEEIAQGGMGRIRAARDLRLGRDVAIKEILEPRGSLATRFEREAMLTARLQHPSIVAV
                     YEAGRWPTGEPFYAMRLVPGTSLKKAITQRASGDERLALLPNVIAIADAMAYAHDQRI
                     IHRDLKPANVLVGDFGETVVIDWGLAKDLDASDGHSDEEAMRASSGELTVAGSIVGTP
                     NYMPPEQAAGDPVDERADVYAIGALLYHVLAGVPPYVGDNAKEILEKVAKEPPPPITT
                     LQKGIAPDLVAIVERAMSREVARRYPSARELAEDLKRFQTGQLVGSHRYSRRELISRF
                     VRRYRTPLAITSVAAIIVAAIGVLAVQRIRHERDQSVLESHRADRERDTAERERAKAA
                     ERADSLTIEQAASSLERDPRSALQLLQGLSSGSSQWTAARLLAADAASRGIARPLYRG
                     DVQTLAVSPDGKQLAIADQANALLLVDLATGHTKQIGSHRDVIEKLTFAKNGRALLSR
                     SRDHDVTVWDLASSRVTTLPHDSPVGGAHISEDGTYVITIEFSKRARLWEVASAAAKV
                     DVQTKEGMVFLPSDAGVRALYETDAGFAVVNAATGGVEQVFRNKSKLLDNVSGVSWST
                     NRLQLAFAKHDGVVGIYDIASGTERILGKHGAGPVYSPSYLPGDSEIVTVADGELRVW
                     PVAGGKPRVIGTQPEREAFSFLTVSPTGHHVIVTSGVPRLWNLDTGASALLVGPLGGL
                     RSMQFTPDGTHVLGLAADGVWSWPTQRSDESVERRPEPVRGLAFAGTSLLMTDAAGIE
                     LGTTKLPFKRGIVGRAALAPDGKRIALLGKDGHVHVIDVARQSDEVLEGTLETLRREF
                     PADPKAQTDKKSDVINVRRYTRWTRDRGGWFDPVVFSPNGSAIAAAGTSGGRILLYKA
                     GSPTPITIELGTTDVEQLLFAPDGKTLFVAASDSNVHVISATGADRSTLKHSEIVEAM
                     TLSADGKTLYTACVDRTIRSWFVATGTQRVIGKHPDIATALALTPDGKHLVSAGHRKQ
                     IELWDLATGDSVPLDGLAADPLVIAVSGDGGRLVAGDTQGTVRVWDLASHRGRTLRSR
                     GDAVGALAISPDGTRVVAADDHGQLIRFDDTVPATEPLLRQWIADALR"
     gene            3686..4039
                     /locus_tag="VMZ53_08955"
     CDS             3686..4039
                     /locus_tag="VMZ53_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28626.1"
                     /translation="MKYTLLALLVGLPLLGCKGEDVSVHVDCITTAAPAIECEVKQTK
                     GKSEVEACWDMSVTCANGNVVKAPHWCQKVKDGGTQKTTIPGDKLTGMDGCGGSGPPT
                     AKLENMTINGKASTN"
     gene            4075..4767
                     /locus_tag="VMZ53_08960"
     CDS             4075..4767
                     /locus_tag="VMZ53_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28627.1"
                     /translation="MSMMRVVAPVLLAVLLAAPASANTAPGWNDVPALIAFATQSVDK
                     ELRGCSKRTPPWDIALIATRDPKTGATKVAMPFPNVGHRGFTAEEKCLMAAVPKIALP
                     DLPAGIDRIVVGHTVVADGATAPSPDKAFDTWKDPAATLASFVDDKAKASLAACDKKA
                     RTVRVILDLSHGKTRVWLPAWQFHSAKGDGSTPAAEAKVKACVNKVIATWKPTPLPKQ
                     MAEMQLAISVAP"
     gene            4805..5824
                     /locus_tag="VMZ53_08965"
     CDS             4805..5824
                     /locus_tag="VMZ53_08965"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HUS28628.1"
                     /translation="MFDVRLPIIQAPMAGGTDTPALAAAVSNAGALGSLGCAYLTPAQ
                     IDHAARDVRARTDKPFALNLFARGESAEDPAGDARVNAILAPIREELGLASFSVPPPS
                     LPRFDDQLEAVLRAAPRVFSFTFGVPDSVQLSSMRVRGISIVGTATTVEEARALDDAG
                     VDAICVQGGEAGGHRGTFLGSFDDAVIGTLSLVQQVVRVTKLPVIAAGGIMTGEAIRA
                     VLSLGASAAQLGTAFMCCPEAGTAPAHREALATATTTTITRAFSGRPARGIRNRMTDT
                     FARTDPAPFPQQQRLTAEIRREASKRGRTDLMQMWAGQAAPLVRAMPAAELVATLARE
                     AGLEA"
     gene            complement(5813..6982)
                     /gene="mltG"
                     /locus_tag="VMZ53_08970"
     CDS             complement(5813..6982)
                     /gene="mltG"
                     /locus_tag="VMZ53_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HUS28629.1"
                     /translation="MSKRSFRIALVVVAASVAIIALVLALMARSALNYPEERHAGTGK
                     EIEVEIKSGMSFPAVSKLLAEKHLISRPTWFRLYAMWEGDTTNIKPGKYVIKDNLAPK
                     EVLKIIITGVKELTVTVTLPEGKNMLEFFDLIAKACPKGMVETPEKACPHITTAKELE
                     AVARDKDFLAAHAIVGDTVEGYLFPDTYEFRVGEKPRRVLDRLVEHHQEIFHEIITKN
                     PKDTAKIKDKMRWSDREILTMASIVEKEAVAQAERPKIARVFLNRLTKPDFNPKRLET
                     DPTIRYGCLVPEKKSPACVAWNEPCAKQGQPPGCDRLHRAQLDDEDNPYNTYRHEGLP
                     PGPISNPGRSSIEAVISPDSNDYIYFVAKSPTEHAFAKTLAEHQKNVAAYVKSGL"
     gene            complement(6996..7424)
                     /gene="ruvX"
                     /locus_tag="VMZ53_08975"
     CDS             complement(6996..7424)
                     /gene="ruvX"
                     /locus_tag="VMZ53_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HUS28630.1"
                     /translation="MRALGLDVGSKTIGVAITDEAGIAAHPLRVIERAGTATDVSTIQ
                     TLVVENGITDVVVGMPYELSGKVGHRARRVQEFISALTSALSRELKFHEQDERFTTAE
                     AQRVLIDADLSRAKRKGVIDQQAAALILQGWLDLQARQSK"
     gene            7903..8196
                     /locus_tag="VMZ53_08980"
     CDS             7903..8196
                     /locus_tag="VMZ53_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HUS28631.1"
                     /translation="MSSPDDVPPAPKLQIQIDDDISQGVYSNLVLLNHTENEFVLDFA
                     FIQPANGRAKVRSRVISSPRHTKRLLLALQKNLERFEERYGRIELGPDEELVH"
     gene            complement(8200..>8811)
                     /locus_tag="VMZ53_08985"
     CDS             complement(8200..>8811)
                     /locus_tag="VMZ53_08985"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28632.1"
                     /translation="IIGFSDATALLAWAYAAGVRGIHGPMVAQLPSLAPADTAYLIEM
                     ISDARVPGVRPWQLAAHGKGRYRGPLVAANLTMAAMLIGTPWPLPVRGAITIVEEIGE
                     RPYEIDRYLTQLALAGELAKLSAVVVGDLTRCADANPPTGVPDPDDAALRTILERLHA
                     AGTPAAVGAPIGHGDRNEAVPFGADAILDLDAGTLEILEPAVA"
BASE COUNT         1461 a         2887 c         2901 g         1562 t
ORIGIN      
        1 cgcggcgaag gcgccgacgt cgcgacgatt cgcaccggga acctcgaccg cctgacgatt
       61 cccgacaacg gcgcgtacac gatggcgccg aattcgctga aggcggagaa ggacaagaca
      121 cgcgaaaaca cgatcgagct gcgccgcacc aatcgcgtga cgcctgccgg tgcgctcggt
      181 ggctcgagct tccaagtctc gatcgagaat cgcgtcgacg tgatcgcgct cgcgaacccg
      241 gcgctctagc gcagcgcatc cgcgatccac tggcgcagga gcggctcggt ggcgggaacc
      301 gtgtcgtcga agcggatgag ctggccatga tcgtcggcgg cgacgactcg cgtcccgtct
      361 ggcgagatcg caagggcgcc tactgcatcg ccgcgcgatc gcagcgtgcg gccgcggtga
      421 ctcgcgagat cccagacgcg caccgtgcct tgcgtgtcgc ccgcgacgag gcggccgcca
      481 tcgcccgaga ctgcgatcac gagcggatcc gcagcgaggc cgtcgagggg caccgagtcg
      541 cccgttgcga ggtcccacag ctcgatctgc ttgcgatgac ctgcgctgac gagatgtttg
      601 ccgtcgggcg tgagcgcgag cgccgtcgcg atgtctggat gtttgccgat gacgcgctgg
      661 gtgccggtcg cgacgaacca cgagcggatc gtccgatcga cgcacgccgt gtagagagtt
      721 ttgccgtccg cgctgagcgt catcgcttcg acgatctcgc tgtgcttcag cgtgctgcga
      781 tcggcgccgg tcgccgagat cacgtggacg ttgctgtcgc tcgcggcgac gaacagcgtc
      841 ttgccgtcgg gcgcgaacag cagctgctcg acgtccgtcg tgccgagctc gatcgtgatc
      901 ggggtcgggc tgcctgcttt gtagaggaga atgcggccac cggacgtgcc ggctgctgcg
      961 atcgcggagc cgttcggcga gaacacgacg ggatcgaacc agccgccgcg gtcgcgggtc
     1021 cagcgcgtgt agcgacgcac gttgatgacg tcggacttct tgtccgtttg agctttcgga
     1081 tctgccggaa actcgcgtcg aagcgtctcg agcgtgcctt cgagtacttc gtcggactgg
     1141 cgcgcgacgt cgatgacgtg gacgtggccg tccttgccga ggagcgcgat gcgtttgccg
     1201 tcgggtgcga gcgcggcgcg tccgacgatg ccccgtttga acgggagctt cgtggtgccg
     1261 agctcgatgc ctgctgcatc ggtcatgaga aggctcgtgc ccgcgaatgc gaggccgcgg
     1321 accggctctg gccgtcgttc gacgctctcg tctgaccgct gcgtcggcca gctccaaacg
     1381 ccatcggcag cgagaccgag cacgtgcgta ccgtcgggtg tgaactgcat cgagcgcagg
     1441 ccgccgagcg gaccgacgag gagcgcggac gcgccggtgt cgagattcca caagcgcggg
     1501 acgccgctgg tcacgatgac gtgatggccc gtcggcgata cggtaagaaa cgagaacgcc
     1561 tcacgctcgg gctgcgtgcc gatcacacgt ggcttgccgc cggcgacggg ccatacgcgc
     1621 agctcgccgt ccgctaccgt cacgatctcg gaatcgccgg gaaggtagct gggcgagtag
     1681 accgggccgg cgccgtgctt gccgaggatg cgttcggtgc cggacgcaat gtcatagatg
     1741 ccgacgacgc catcgtgctt cgcgaaggcg agttgcaggc ggttcgtcga ccacgagacg
     1801 ccggagacgt tgtcgagcaa cttgctcttg ttgcgaaaca cctgctcgac gcctccggtc
     1861 gcggcgttga cgacggcaaa gcccgcgtcg gtttcgtaga gcgcgcgcac gccggcatcg
     1921 ctcgggagaa agaccatgcc ttccttcgtc tggacgtcga ccttcgccgc ggcgctcgcg
     1981 acctcccaca gccgcgcgcg cttcgaaaac tcgatcgtga tcacgtacgt gccgtcttcc
     2041 gagatgtgag caccgccgac cggagagtcg tgcggcagcg tcgtaacgcg actgctggcg
     2101 aggtcccaga ccgtgacgtc gtggtctcgc gagcgactca gcagggcgcg accgttcttc
     2161 gcgaaggtga gcttctcgat gacgtctcga tgcgagccaa tctgcttcgt gtgtccggtg
     2221 gccagatcga cgagcaagag cgcgttggcc tggtctgcga tcgcgagctg tttgccgtcg
     2281 ggagaaacgg cgagcgtttg cacatcaccg cgatacagag ggcgagcgat gccccgtgac
     2341 gcggcgtccg cggcgagcaa gcgcgcagct gtccactgcg acgagcccga cgacaggcct
     2401 tgcagcagct gcagtgcgct gcgcgggtcg cgctcgagcg acgaggcggc ctgctcgatc
     2461 gtcagactgt cagcgcgttc ggccgccttc gcgcgttccc gttctgcggt gtcgcgctct
     2521 cgatcggcgc ggtggctctc caacacggat tgatcgcgct cgtggcgaat gcgctgcact
     2581 gcgagaacgc cgatcgccgc gacgatgatc gcagcgacgc tggtgattgc aagcggcgtc
     2641 cggtagcgac gcacgaagcg cgagatcagc tcgcgccgcg aatagcgatg tgagccgacg
     2701 agctgtccgg tttggaagcg cttgagatct tcggcgagct ctcgtgcgct cggataccga
     2761 cgagccacct cgcgagacat cgcgcgctcg acgatggcga cgagatcggg tgcgatgccc
     2821 ttctgcagcg tcgtgatcgg gggcggtggc tccttcgcga ccttctcgag gatctctttc
     2881 gcgttgtcgc cgacatacgg cggcacgcct gcgaggacgt gatagagcag cgcaccgatc
     2941 gcgtagacgt ctgcgcgttc gtcgaccgga tcgcccgccg cttgctcggg aggcatgtag
     3001 ttcggcgtgc cgacgatgct gcccgcgacc gtcagctcgc cggagctcgc ccgcatcgct
     3061 tcctcgtccg agtggccgtc gctggcgtcg agatccttcg cgaggcccca gtcgatgacg
     3121 acggtctcgc cgaaatctcc gacgagcacg ttcgccggct tgaggtcgcg atggatgatg
     3181 cgttggtcgt gcgcgtacgc catcgcgtcg gcgatcgcga tcacgttcgg caagagtgcg
     3241 agtcgttcgt cgccgcttgc tcgctgcgtg attgctttct tcagcgacgt cccggggacg
     3301 agacgcatcg cgtagaacgg ctccccggtt ggccacctgc ccgcttcgta gacggcgacg
     3361 atcgatggat gctgcagtcg cgccgtcagc atcgcttcgc gttcgaagcg cgtcgcgagt
     3421 gagccgcgcg gctcgaggat ctctttgatc gcgacatcgc ggccgaggcg caggtcgcgc
     3481 gcagcccgaa tccgccccat gccgccctgc gcgatctctt cgccgaggtc gtagaggctc
     3541 ggatcgatcg tcggcaagcc gagggtgcga ggtcgaacct ggaccgtccg cgagcggtcg
     3601 acatcggtga gcgtttcgtc caacgccacc gtcagcttgt cgtccgaacc cgccatctct
     3661 cgtcgatctt atgtagcatc gggtcatgaa gtacacgctt cttgcgcttc tcgttggcct
     3721 cccactactc ggctgcaaag gcgaggatgt gtccgttcac gtcgactgca tcaccacggc
     3781 tgcgcccgcg atcgagtgcg aggtgaagca gaccaagggc aagtccgagg tcgaggcgtg
     3841 ctgggacatg agcgtcacgt gcgcgaacgg caacgtcgtc aaagcgccgc actggtgcca
     3901 gaaagtcaaa gacggtggca cgcagaagac gacgattccc ggcgacaagc tgaccggcat
     3961 ggacggctgc ggtggcagcg gcccgccgac ggcaaagctc gagaacatga cgatcaacgg
     4021 caaagcgtcg acaaactgag cgcggcgcga cacctcgcgc tatcacggtg acacatgtcg
     4081 atgatgcgtg tcgtcgcgcc cgttctgctc gccgtgctgc tcgcggcccc tgcttccgcg
     4141 aacaccgcgc cgggttggaa cgacgtccct gccctgatcg ccttcgcgac gcaatccgtc
     4201 gacaaggagc tgcgcggttg cagcaagcgc acgcccccgt gggacatcgc gctgatcgcg
     4261 acacgcgatc cgaagacggg cgcgacgaag gtcgcaatgc cgttccccaa cgtcggccat
     4321 cgcggcttca cggccgagga aaaatgtctg atggccgcgg ttccgaagat cgcgcttccg
     4381 gacctacccg cgggcatcga ccggatcgtc gtcggccaca cggtcgtcgc cgacggtgcg
     4441 accgcgcctt ctcccgacaa agcattcgac acgtggaagg atcccgctgc aacgctcgcc
     4501 tcgttcgtcg atgacaaggc gaaggcctcg ctcgccgcgt gcgacaagaa agctcgcacg
     4561 gtgcgcgtca tcctcgacct cagccacggc aagacgcgcg tgtggctgcc cgcgtggcag
     4621 tttcactcgg cgaaaggcga cggctcgaca ccagctgccg aggcaaaggt gaaggcgtgc
     4681 gtcaacaagg tgatcgcgac gtggaagcca acgccgctgc cgaagcagat ggccgagatg
     4741 cagctcgcga tttcggtcgc gccgtagagc gcaacgatcg cgcggttcgc ggcatcatgc
     4801 gtgcgtgttc gacgttcggc tcccgatcat tcaggcgccc atggccggcg ggacggatac
     4861 gcccgcgctt gctgctgccg tgtcgaacgc cggcgcgctc ggctcgcttg gctgcgccta
     4921 cctaacgcct gcgcagatcg accacgcggc tcgcgacgtg cgcgcgcgga ccgataagcc
     4981 gttcgcgctg aacctgttcg cgcgtggtga gtcggcggag gatcccgccg gtgatgctcg
     5041 cgtcaatgcc atcctcgcgc cgatcagaga ggagcttggc ctcgcgagct tctccgtccc
     5101 gccgccatcg ctgccgcggt tcgacgatca gctcgaggcc gtgctgcgtg ctgcaccgcg
     5161 cgtcttcagc ttcacgttcg gcgtgcccga ttcggtgcag ctctcgtcga tgcgcgtccg
     5221 cggcatctcg atcgtcggca cggccacgac cgtcgaagaa gcgcgcgcac tcgacgatgc
     5281 aggcgtcgac gccatctgcg tgcagggcgg cgaagccggt ggccatcgcg ggacgttcct
     5341 cggctcgttc gacgacgccg tgatcggcac gttgtcgctc gtgcagcagg tcgtgcgcgt
     5401 cacgaagctg cccgtcattg cggctggcgg catcatgacc ggcgaagcga tccgcgcagt
     5461 gctgtcgctc ggcgcgagcg ccgcgcagct cggcaccgca ttcatgtgct gccccgaggc
     5521 aggtactgcc cccgcgcacc gcgaagcgct cgcgaccgcc accacgacga cgatcacgcg
     5581 cgcattttcc ggacgccccg cgcgcggcat tcgcaatcgc atgaccgata cgttcgcccg
     5641 caccgatcct gcgcccttcc cacagcagca gcgactcacg gcggagatcc ggcgcgaagc
     5701 gagcaagcgc ggccgcaccg acctcatgca gatgtgggca ggccaagcgg cgccgctcgt
     5761 tcgcgcaatg cccgcggccg agctcgtcgc cacgctcgcg cgcgaagctg gcctagaggc
     5821 ctgacttcac gtacgccgcg acgttcttct gatgctccgc gagcgtcttc gcgaacgcgt
     5881 gctcggtcgg gctcttcgcg acgaagtaga tgtagtcgtt gctgtcgggt gagatgaccg
     5941 cctcgatcga tgaccgaccg ggattgctga tcggtccggg cggcaagcct tcgtggcgat
     6001 acgtgttgta cgggttgtcc tcgtcgtcga gttgcgcgcg gtgcaggcga tcgcagcccg
     6061 gcggctgacc ctgctttgcg cacggctcgt tccacgcgac gcacgcgggg gacttcttct
     6121 cggggacgag gcagccgtag cggatcgtcg gatctgtctc gaggcgcttc gggttgaagt
     6181 ccggcttcgt gagccggttg aggaacacgc gcgcgatctt cgggcgctcg gcctgcgcga
     6241 cggcttcctt ctcgacgatc gatgccatcg tgaggatctc gcgatcgctc cagcgcatct
     6301 tgtctttgat cttcgccgtg tccttcggat tcttcgtgat gatctcgtgg aagatctctt
     6361 ggtggtgctc gaccagccga tcgagcacgc ggcgcggctt ctcgccgacg cggaactcgt
     6421 acgtgtcggg gaacaagtag ccctcgaccg tgtcgccgac gatcgcgtgc gcggcgagga
     6481 agtctttgtc gcgcgcgacg gcttcgagct ctttggccgt cgtgatgtgc gggcacgctt
     6541 tctccggcgt ctcgaccatg cctttggggc acgccttcgc gatgaggtcg aagaactcga
     6601 gcatgttctt gccctctggc agcgtcaccg tgacggtgag ctccttcacg cccgtgatga
     6661 tgatcttcaa gacctccttc ggagcgaggt tgtctttgat gacgtacttg cccggcttga
     6721 tgttcgtcgt gtcgccttcc cacatcgcgt agaggcgaaa ccacgtcggc cggctgatga
     6781 ggtgtttctc ggcgagcagc ttgctcaccg ccgggaagct catgccgctc ttgatctcga
     6841 cctcgatctc tttgcccgta cccgcgtgcc gctcctccgg atagttgagt gcgctccgcg
     6901 ccatcaacgc caggaccagc gcgatgatcg cgaccgatgc ggccacgacc acaagcgcga
     6961 tgcggaagga gcgtttcgac atccggaagc tgaccttact tcgactggcg cgcttgcagg
     7021 tcgagccacc cctgcagaat gagcgcggcg gcttgctgat cgatcacgcc cttgcgcttc
     7081 gcgcgtgaca gatcggcgtc gatgagcacg cgctgcgcct cggctgtcgt gaaacgctcg
     7141 tcttgctcgt gaaatttcag ctcgcgactg agcgcgctcg tgagcgcact gatgaattct
     7201 tgtacgcgcc gcgcgcgatg cccgaccttg cccgacagct cgtacggcat tccgacaacg
     7261 acatcggtga tgccgttctc gacaacgagt gtctggatcg tcgacacatc cgtcgcggtc
     7321 cctgcgcgct cgatcacgcg gagtggatgt gcagcaatcc cagcttcatc ggtgatcgcg
     7381 acaccgatgg tcttgctgcc gacatcgagg ccgagcgcac gcattgcgcg ttcgagactg
     7441 cccgggattc cggaaaaaaa aaggccctgc aagggggatt gcagggcctg ggcgcagatc
     7501 gaagtagcct acttgttgcc tcagatggtg ggggagacta gcgggatgag gggggaggga
     7561 accgccagtc atccatcgag tcgaaacgtt ggactactcc gtcgcgcgtg ggattcgatt
     7621 tagttttcac aaccgagaag ctcgccggtc ctattccttg gaacgtccga ggaggctctt
     7681 ggttgcatga aactatgagc acattcgatg ccaccgaaat tcgagatcac tccgacgagg
     7741 aatcgcccat ttacgaggcc gctgtgtcct gaacggcaac aagtcctgtc acccctggtt
     7801 ctcagaactg gcgagtctgg tggccaccca acgaaccgca atcacgaatg atcgcggttc
     7861 aaccgcgcaa ctgcacaaca cgcgcacggt aacgtggcgg caatgtcctc cccagatgac
     7921 gtcccgcccg caccgaagct ccagatccag atcgacgacg acatctcgca gggcgtttac
     7981 tccaacctcg ttctgctcaa tcacaccgag aacgagttcg tgctcgactt cgcatttatc
     8041 cagccggcaa acggccgcgc aaaagtgcga agccgcgtga tctcatcacc acgccacacg
     8101 aagcgactct tgctcgcgct gcagaagaac ctcgagcgct tcgaagagcg ctacggccgc
     8161 atcgagctcg gtccggatga agagctcgtt cactagccgt tacgcgacgg cgggctcgag
     8221 aatctcgagc gtcccggcat cgagatcgag aatcgcgtcc gccccgaacg gcaccgcctc
     8281 atttcgatcg ccgtgaccga tcggcgcgcc gaccgcggct ggcgttccgg cagcgtggag
     8341 ccgctccaag atcgtccgca gcgccgcatc atctggatcg ggcacgcccg tcggtgggtt
     8401 tgcatcggcg cagcgggtga gatcgccgac gaccaccgca gacagctttg cgagctcgcc
     8461 cgcgagcgcg agctgcgtca ggtagcgatc gatctcgtag ggccgctcgc cgatctcttc
     8521 gacgatcgtg atcgcaccgc gcaccggaag cggccacggc gtaccgatca gcatcgctgc
     8581 catcgttaga ttcgccgcga cgagcggacc gcgatagcga cctttgccat gcgcggcgag
     8641 ctgccatggc cgcacgcccg gaacgcgcgc atcactgatc atctcgatga gatacgcggt
     8701 gtctgccggc gcgagcgaag ggagctgcgc aaccatcggg ccgtgaatac cgcgaacgcc
     8761 cgcggcgtat gcccacgcga gcagcgccgt cgcgtcggag aagccgatga t
//
LOCUS       DATLBZ010000170         4456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4630316, whole genome shotgun sequence.
ACCESSION   DATLBZ010000170 DATLBZ010000000
VERSION     DATLBZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4630316"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VMZ53_08990"
     CDS             complement(<1..657)
                     /locus_tag="VMZ53_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28633.1"
                     /translation="MPQRSVLLLALVLATVTLVVQARVILGGKTFDDVTYHTQVAPPR
                     YAAATQVQEGSLPTWWDGSGLGVPLLGEPSHGAAYPLGWVAGTPRALELVLLLHLFIA
                     ALGTALWSRKNGASDVAALVCGVLVATSGVAASALLRGALPALAWLPWIAWARTSDRY
                     ALLGAFLALVGLAGDLALLIDAVALALLVRPATPDVASARADVASARADVAGGRADVA
                     G"
     gene            719..1069
                     /locus_tag="VMZ53_08995"
     CDS             719..1069
                     /locus_tag="VMZ53_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28634.1"
                     /translation="MLRALVLTLPLLVSCHRKSADDAPCATVAGRMFTLAKTDLGSAS
                     VDPATRRAVADQLPAMRDSLTQICTDGKWSTQVRNCMVNAADHVAMQACQRELTDEQR
                     RALDLAARGETTSR"
     gene            1093..1401
                     /locus_tag="VMZ53_09000"
     CDS             1093..1401
                     /locus_tag="VMZ53_09000"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HUS28635.1"
                     /translation="MEIDFQYALSDPDAKSRLEILGHYLGNRHGIKVTWVDPQRAKFS
                     GKYLVVTINGELTVGNGKACFKGEDPGFLWRSRAKDYIQGKLAKYLDPKLQPTELPTS
                     "
     gene            complement(1456..2223)
                     /locus_tag="VMZ53_09005"
     CDS             complement(1456..2223)
                     /locus_tag="VMZ53_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HUS28636.1"
                     /translation="MKLATWNINSIRARTERLLTWLGAERPDVLCLQETKVEDSGFPL
                     AAIEKAGYHVAMHGQRSYNGVAILSRTALTDVTRGFGDGEPEEEARVISARVGDLRVV
                     SIYVPNGQDLESEKFPYKLGWYKRLRAYLDRTATTSDQLVVCGDTNVTADDRDVWSPE
                     AWAGKIHCSPKEREALANVIGFGLVDLFRKHHPDGGVYSWWDYRGVSFFKNQGLRIDH
                     ILATPPVAERCTACVIDRNARKGQDASDHAPVIATLA"
     gene            2357..2494
                     /locus_tag="VMZ53_09010"
     CDS             2357..2494
                     /locus_tag="VMZ53_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28637.1"
                     /translation="MKHQPDLSVWSIIERLQRRLERQGMSPAAADRAVAIALRQLAHA
                     R"
     gene            complement(2525..3352)
                     /locus_tag="VMZ53_09015"
     CDS             complement(2525..3352)
                     /locus_tag="VMZ53_09015"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUS28638.1"
                     /translation="MTAVFFGAFCVYGLLHAVGKPPQVVAVKHNQFFGPFLAGYVVWM
                     LGPVERVMVGRVSPNFITAMSLLMCIATGVAAGMHHLGTAVWLFVFAGIFDIMDGRIA
                     RLAGMQTKSGALFDSVSDRWGELVAFGGYAWYLHDSPWMLSVFAALGASTMVSYTRAR
                     AEGLGIELSGGMMQRAERMVLVAGGTLVAAWYGANPESASAAMPILGVTMLVCAVTAT
                     ATALNRWLVAYRQLAAREAAAIEAATPEPAELPVPVPARISALSSPDLRKVRPLEQH"
     gene            3461..3994
                     /locus_tag="VMZ53_09020"
     CDS             3461..3994
                     /locus_tag="VMZ53_09020"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HUS28639.1"
                     /translation="MASISQRVEKTANRSMFRTLNGLSKVIATAGGAGYSPIAPGTCG
                     TAVAVPLAYLLRGLPIWQFAIVTAAITALGIWAADRADRAWGTHDSGRIVIDEVAGYL
                     VTMTLVSRGHWAPLAVGFVVFRALDIIKPPPVRWLDENLPGGWGVVLDDIAAGVIGMA
                     LMVALDRFGALDALAGL"
     gene            4002..>4456
                     /locus_tag="VMZ53_09025"
     CDS             4002..>4456
                     /locus_tag="VMZ53_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28640.1"
                     /translation="MRWASVAVAAAVLAAPLAAHANGRAPLTNGITFKPGDDQSIYLA
                     TTFGLLVSHDDGCTFRWICEQNIGYGGQWDPKYAIATDGTIFATTFEGLRISRDGGCS
                     FTTATADLAAIDPNRIADVWIDALDIGPTGDVWVGTAETGHTNDIFVSH"
BASE COUNT          824 a         1477 c         1431 g          724 t
ORIGIN      
        1 accagcgaca tccgcgcgcc caccagcgac atccgcacgc gccgaggcga catccgcacg
       61 cgccgaggcg acatccggcg tcgcggggcg gacgagcagc gcgagcgcga ccgcatcgat
      121 gagcagcgcg agatcaccgg cgaggccgac gagcgcgagg aacgcaccga gcagcgcgta
      181 gcgatcgctc gtgcgcgccc acgcaatcca cgggagccac gcgagcgccg gaagcgctcc
      241 gcgcagcagc gcgcttgcgg ccacaccgct cgtcgcgacg agcacgccgc acacgagagc
      301 agcgacgtcg ctcgctccgt tcttgcgcga ccacagtgcg gtgccgagtg ccgcaatgaa
      361 gaggtgcagc agcagcacga gctcgagtgc gcgcggcgtg cctgcgaccc agccgagtgg
      421 atacgcggcg ccatgcgatg gctcgccgag cagaggcacg ccgaggccgc tgccgtccca
      481 ccacgtcggg agcgatccct cttggacctg cgtcgccgcg gcgtaccgag gtggtgcaac
      541 ttgtgtgtgg tacgtgacgt cgtcgaacgt cttgccgccg aggatcacgc gcgcctggac
      601 gacgagcgtc accgtcgcaa gcacgagcgc gagcaagagg accgaacgct gcggcaccgc
      661 gctgactata gaaggtgcga tcttgacagc ttccgccgcg gggtcgaaaa cgacgctcat
      721 gttgcgcgcg ctggtcctga ccctgccgct tcttgtcagc tgccaccgta agagcgcgga
      781 tgacgcacca tgtgcgacgg tcgccgggcg gatgttcacg ctcgccaaga ccgacctcgg
      841 ttcggcctcc gtcgatcctg cgacgcggcg tgccgtcgcc gatcaactgc ccgcaatgcg
      901 cgactctctc acgcaaattt gtacagatgg gaaatggtct acgcaggtgc gtaattgcat
      961 ggtcaacgct gccgaccacg tcgcgatgca ggcgtgccag cgcgagctca ccgacgagca
     1021 acggcgagcg ctcgaccttg ccgcacgtgg cgagacgact tcgcgctgac cgtgctaaaa
     1081 attagtccag gcatggagat cgattttcag tacgcgctct ccgacccaga tgcgaaatct
     1141 cgcctcgaga ttctgggcca ctacctcggc aaccgtcacg gcatcaaggt gacgtgggtt
     1201 gatcctcagc gcgccaagtt cagcggcaag tatctcgtcg tgacgatcaa cggcgagctc
     1261 accgtcggta acggcaaggc gtgcttcaaa ggcgaggacc ccggttttct gtggcgcagc
     1321 cgcgccaagg attacatcca gggcaagctc gccaagtatc tcgatccaaa gctccagccg
     1381 accgagcttc cgaccagcta gctcgaagcg taagcgtaag cgaccgtcgc gaacggttca
     1441 gggtctgcgt aattctcacg cgagcgtcgc gatgaccggc gcgtgatccg acgcgtcttg
     1501 gcctttgcgc gcgttgcgat cgatcacgca tgcggtgcag cgctccgcga ccggcggtgt
     1561 cgcgagaatg tgatcgatgc gcaggccttg gttcttgaag aacgagacgc cgcggtagtc
     1621 ccaccacgag tacacgccgc cgtcgggatg atgcttgcgg aacagatcga cgaggccaaa
     1681 gccgatcacg ttcgcaagcg cctcgcgttc cttcgggctg caatgaatct tgcctgccca
     1741 cgcttcgggt gaccacacgt cgcgatcatc cgccgtgacg ttcgtgtcgc cacatacgac
     1801 gagctgatcg ctcgtggtcg cggtgcgatc gagatacgcg cggagccgtt tgtaccaacc
     1861 gagcttgtac ggaaacttct cggactcgag gtcctgacca ttcggcacgt agatcgaaac
     1921 gacgcgcaga tcgccgacgc gcgccgagat cacgcgcgct tcttcttccg gctctccatc
     1981 gccgaagccg cgcgtcacat ccgtgagcgc agtgcgcgag agaatcgcga ccccgttgta
     2041 gctgcgctgg ccgtgcatcg cgacgtggta gccagccttc tcgattgcgg cgagcggaaa
     2101 gccgctgtcc tcgaccttcg tctcctgcag acagagcacg tcgggccgct cggcgccgag
     2161 ccacgtgagc agacgttcgg tgcgtgcgcg gatcgagttg atgttccagg tcgcgagctt
     2221 cacgaggccg cagcctacat cgccccaccc ggtcggggag cttccgtgca accgcgaagc
     2281 gccgcatctg tcctagggaa tgtgggcggg cgatcggttg ttagacccac agccgccctc
     2341 gtacgtgagc atcaccatga agcatcaacc agacctatct gtgtggagca tcatcgagcg
     2401 ccttcagcgc cgcctcgagc gccaggggat gtccccggct gctgccgatc gcgccgtcgc
     2461 gatcgctctc cgccagctcg cgcacgctcg ctagctacgc cgcctgcggc aacaggcgtg
     2521 acggctagtg ctgctcgagc ggacgaacct tgcgaaggtc cggcgacgac agcgcactga
     2581 tgcgagcggg aacggggact gggagctctg caggctcggg cgtcgcggct tcgatggccg
     2641 ccgcttcgcg agccgctagc tggcgatacg cgacgagcca gcggttgagc gccgtcgccg
     2701 ttgcggtgac cgcacatacg agcatcgtga cgccgaggat cggcatcgcc gccgacgcgc
     2761 tctcgggatt cgcgccgtac caggcagcga cgagtgttcc accggcgacg agcaccatgc
     2821 gctctgcgcg ctgcatcatg ccgccagaca gctcgatgcc gaggccttct gcgcgagcgc
     2881 gcgtgtagct caccatcgtc gatgcgccga gtgcggcgaa cacgctgagc atccacggcg
     2941 agtcgtgcag gtaccaagcg tagccgccga acgcgacgag ctcgccccag cgatcgctga
     3001 cggagtcgaa cagcgcgcct gacttcgtct gcatcccggc gaggcgcgcg atgcggccgt
     3061 ccatgatgtc gaagatgccc gcgaacacga acagccacac cgcggtgccg aggtggtgca
     3121 tgccggccgc aacgccggtc gcgatacaca tgaggagcga catcgccgtg atgaagttcg
     3181 gcgacacgcg gccgaccatc acacgctcga ccggtcccaa catccagacc acgtagcccg
     3241 cgaggaacgg gccgaagaac tggttgtgct tgacggcaac tacttggggc ggcttaccga
     3301 ccgcgtgaag cagcccgtag acgcagaaag cgccgaaaaa gacggcggtc aaaagtagtg
     3361 caggcgctag tagataaaac ctatccacgc ttctctctaa gaaaaggaac tacctgcagt
     3421 tgggttatca cgtaagtaca ggagcgtcga tggtcaaagg gtggcatcga tctcccaacg
     3481 tgtcgaaaaa acagcgaaca ggagtatgtt tcgaaccttg aacggtcttt ccaaggtgat
     3541 cgcgacggcc ggcggcgcgg gttattcacc gatcgcccct ggaacgtgcg gaaccgctgt
     3601 agcagtaccg ctcgcttacc tgctgcgggg cctcccaatc tggcaatttg cgatcgtcac
     3661 ggcggcgatc accgccctcg ggatctgggc ggccgaccgg gccgatcggg cgtggggaac
     3721 gcacgacagc ggccgtatcg tgatcgatga ggtcgccggc tacctcgtga cgatgacgct
     3781 cgtcagtcgc ggccattggg cgccgctcgc agttggcttc gtcgtatttc gcgcgctcga
     3841 catcatcaag ccgcctcccg tccgctggct cgacgagaac ctgcccggcg gctggggcgt
     3901 ggtcctcgac gacatcgcag cgggtgtgat cggcatggca ctgatggtgg ccctcgaccg
     3961 tttcggcgcg ctcgacgcgc tcgcgggctt atagtcgcgc catgcgttgg gcctctgtag
     4021 ctgtggcagc cgcggtcctc gccgcgccgc tcgccgcgca cgcaaacggc cgcgcaccac
     4081 tgaccaacgg gatcacgttc aagcccggcg acgatcagtc gatttatctc gcgacgacgt
     4141 tcggccttct cgtctcgcac gacgacggct gcacgttccg ttggatctgc gagcagaaca
     4201 tcggctacgg cggccagtgg gatccgaaat acgcgatcgc gaccgacggg acgatcttcg
     4261 cgacgacgtt cgaaggtctt cgcatcagcc gcgatggagg ctgcagcttt acgacagcga
     4321 ccgccgacct cgccgcgatt gatccaaatc gcatcgccga cgtctggatc gatgcgctcg
     4381 acatcggtcc gacgggcgac gtctgggtcg gcaccgcgga gaccggccac acgaacgaca
     4441 tcttcgtctc gcacga
//
LOCUS       DATLBZ010000171        10584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4631026, whole genome shotgun sequence.
ACCESSION   DATLBZ010000171 DATLBZ010000000
VERSION     DATLBZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10584
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4631026"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1037)
                     /gene="secG"
                     /locus_tag="VMZ53_09030"
     CDS             complement(195..1037)
                     /gene="secG"
                     /locus_tag="VMZ53_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HUS28641.1"
                     /translation="MSTLITILHSIVSLFLILTILLQSGKGGGMGGAFGGSNAGTVFG
                     GSGASTFLRKMTAVAATIFMSTSIVLAFLASHDSSDALEKFGRSQADLAKKKEEAKEK
                     ALLEGSGAGGSGASLGSGGASSMGLPGADTPDLPATTPTPTDTPSTGSAATGSATTGS
                     ATTTPTTTTTTTPTPASGTTSGTATTPPATKTPGGTAAGNSSVVDPTRTGSAGIGMPN
                     TPGTAPKTTTPPAPKTTTPKTDTSVTPSTTPAPKATDTTGSQNATGSAAPSSSTPNSP
                     KPNE"
     gene            complement(1082..1849)
                     /gene="tpiA"
                     /locus_tag="VMZ53_09035"
     CDS             complement(1082..1849)
                     /gene="tpiA"
                     /locus_tag="VMZ53_09035"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HUS28642.1"
                     /translation="MSRTPFVVGNWKLNKTIAEALALVTELKNQLGAVKGVMVGVAPT
                     FVAIQPVAKRLEGSSIVTCSQDCFWESTGAFTGEVSAHFLADAGATWTIVGHSERRQF
                     FGDNNDSVGRKARAVLGAGLGAIVCVGEMLAERDSGRTLEVVDAQLEGGLSGIDAQVA
                     AQRLVIAYEPVWAIGTGRTATPAQAQEVHAHIRKRLADRWGVDTANSIRIQYGGSVKA
                     NNAEALMAEPDIDGALVGGASLEAADFVAIVKAARPA"
     gene            2025..2366
                     /locus_tag="VMZ53_09040"
     CDS             2025..2366
                     /locus_tag="VMZ53_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28643.1"
                     /translation="MDPLDQPVTLRMLNAALDASEQRLAQRLEQRLDQRLDQRLDAFE
                     KRVDARIDDTLKRGLDAGFERMEALFGVVDDKYKHIPPKVAVLEELEPRVARLERAVF
                     APKPKRRRKSA"
     gene            complement(2368..5193)
                     /locus_tag="VMZ53_09045"
     CDS             complement(2368..5193)
                     /locus_tag="VMZ53_09045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUS28644.1"
                     /translation="MAAKRLGVFDPSGRLIDDLAEVADRAHAELNHVDSPETRTNSLA
                     ALLVAPAVASDLGPPPDGAAPRWIVGDGTNAARVAGAAASASAMGVLLTPVSPEALAA
                     IAQPETVAAELDLARARGLIATSLVDLTGAASDTLRSVAEGFAANDCIVWWKDGAQMT
                     PTASRPPPVDGYRATIAAASRIAAASGGTVIVGGPQPRSVIADALRSGPTEVAGLVAI
                     VADSGRRFSAAERTDLKAIAARLTRELSWLTSHRRIVAEGERLLATSLHDPLSGAMTR
                     GAFEQTITHEVAAAGRRNEKLTLALFDVVGLRRINLQHGHKAGDEVLAQIAARVRATV
                     RGNDSIGRPGGDELAVLLVGANEDQAVLVARKLLFKVQNEPVKIDDETTLGVQVRAVL
                     TEIGVGERSGEAALARCYGALRTAPIGDVRIISPDDRAIQDRDVHADTGSLSAGTTVG
                     GTYRVVHELSRGAMGVVYRGEDLGLGRPVAIKVLRSDLASDRDLVDRFRAEAGILASL
                     HHKNLVQVYALGEHAGEVYFVMELVEGQPLSEVLRATLERREWFPTEAVAQIALEIGD
                     ALDSMHALGLIHRDVKPANILLDRERDRAVLVDVGVAVKAGNQSDAAGTPGFAAPESF
                     LEQEGGPQTDVYGLASTMYCALTGRPPFGSGPAPQVVQRQLNDPMGPPSLLRHGLSEA
                     VDVVFRKALLPNPKKRWASASTFAIALGRALERMSGEIRVPVSDADVAALQAESVLGP
                     TQGIEEASNVVALSALRPEIKGRVRAAHLRVLSKVLQHHIGESGMARITNEHPELANA
                     LTSTLAPLAWVELSELVTALDCAIGVLPNALVPRKVGRGTMSATFARLFGADPTSLSP
                     ETVLAALPTFWDRYHDWCEVEVAVHNGAEVRLHGFPGSPHVCALVGAELERIVELTGA
                     EQVSASHPRCQTAGQTACEFKLTWSRG"
     gene            5219..8011
                     /locus_tag="VMZ53_09050"
     CDS             5219..8011
                     /locus_tag="VMZ53_09050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28645.1"
                     /translation="MAERTGDADATEADAVDAPPTEATKREGHLHSDRGARRSDPEYM
                     TRGTLIGRYVVVEKLGEGGMGVVYSAYDPELDRKVAIKLLQARPGGSESAGGQAWLLR
                     EAQALARLQHPNVVGVHDVGTLPGDRVFVAMELVEGKTLRKWLKAEKRSWRALVPVLV
                     EAGKGLVAAHAAGLVHRDFKPDNVLVGDDGRVRVMDFGLARLRRGADTGPIPRVSATD
                     LLVDAKSPLTDSLTMVGSVIGTPAYMAPEVYDGTPADAVTDQFAFGVTAFEALFGSRP
                     FTKEQLTPPRAEVPKPKLPDHTNVPARIQRAVLRAVAIDPAQRFPSVEAFVAELQVDP
                     MKGRKRALIAGGVLATAIGGFAISLAFKNDAPPPCTGIEDQLAEAWSPSVKEKVKAGF
                     VATKKPLAEANYNLVAQALDRYAAEWTQTAIGSCKATRVTKNQTEAVLQLRQGCLDQR
                     RHELKALAAVLAEPSDVLVDKADKAVFELEPVASCNDVEVLAAPGDPPKDVVPKLEKL
                     GEMLALARAQVIGEQIFPAMVSSDKALKEARTVGWPPAVAEALLVRASAMSRTGNSQE
                     AAAMQLEATWMAIRARRDDLTASSALSAAMQVSDALGRPGEAKIYVQLAEEFAKRAGL
                     DKRLEPRINSVRGMVAAQSGDLLGAVDAHQKSFEAAQQLLGAESPALWSDEYMYATTL
                     TTALSYERAVGHYEHAMKLRKQTVGAEHIDIALLESNLGTCYGHLGQVDKAIAAFDRT
                     IAIRQKLFGKNNPLLVVPLVNYADILNREHQAEKALPLIERAVALSVPLPGVEHPTYH
                     QAATTRAEILASLGRISEARKLTEDVMAIETRLKSPTLPTTQASRAEIELAAKEYATA
                     ATYAEQSIAGFEAASGVDNPELWRPLTLLAKAKLGAGDNAAAKPLLERAVAIAAKAGL
                     SEHDLVETKSLLASLP"
     gene            complement(8003..9985)
                     /locus_tag="VMZ53_09055"
     CDS             complement(8003..9985)
                     /locus_tag="VMZ53_09055"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS28646.1"
                     /translation="MSQPTDYRPLVWITDDSAMEAMYTQRSLGTGYDFELFNDGSAVI
                     ERLSKGGRQPDVLLLDWVMPSVSGDEVCRFLRSQPQTRDLPIIIVTASRIETRDVVEG
                     LSIGANDYIPRPFVPEELRARVDNAIRAKQMREAAARERVRLAVIGKLGRAFIDIGPR
                     PTAIVQALATNLIDGICDGCTITLITGALPGLTLARHRTRRDEQLLTTFSAMDPCSYV
                     FASSAEARAKLPAAYHAAIDHFGMTALAVVPFPSGSPVTGVVTAMRDGHSVPFSEEDL
                     VTIQTCLEYAAIAFENALRSEAERETRMQLQTILEHLPISILVADQAGSITHINQIAL
                     DHLPQLRTMRALSDLAAHVSLRTQTGEAIAPSETPMLRALRGEVTRGVELDLNVSERD
                     TKIIRLSATPLRDLRNNVTSAIVAFDDITAERQAILERERAAEFQRYVLGIVSHDLRS
                     PLQTLMMGAETIKLLSPDNAKVSVTLDRMGIATSRMQGIIEQLLDVVRTQLGGGIPLH
                     PAEVNLGSIVVDVLDELTVAYPQAKFEPRLDNVRGMWDRERLAQVVTNLVGNAIIHGA
                     PGSPVVIETKQIDDVAALRVCNASKVALTNEQQAVLFTPFRQTKRSGGSGLGLGLYIA
                     REIVRAHQGDIAVTSNPETTTFTVRLPVSPPPAPLG"
     gene            complement(9969..>10584)
                     /locus_tag="VMZ53_09060"
     CDS             complement(9969..>10584)
                     /locus_tag="VMZ53_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HUS28647.1"
                     /translation="NELQSPMAALARVQRADLVLIGTSTGGPQALTRLLAELPGDLAS
                     PVAMVLHIPIGYTEALAKRLDGVSKLEVVEAHHGVELVPGRAVLARAGQHLKIRRLGS
                     GFVGHLDPLPISPHVPAVDQLFTSGAEAAGSGALGVVLTGMGDDGLIGSRAIAQAGGS
                     LITEAPASCVVYGMPRVVFEAGIGARAAALDYIPREIIRHVTAD"
BASE COUNT         1745 a         3424 c         3707 g         1708 t
ORIGIN      
        1 ccctgatact tcgcaaagcg catacgccca gaccctagcg cggttgtcgc aagagatcac
       61 ctgcgccgat cggggagcgg gcgaaccggt ggcaccgccg gtagatggcg aaaaacagag
      121 caacgccgct cagggatcac cgccaccgtc actggcggag ctacggcgga gctccgacgg
      181 agcgcgacgc gcgattactc gttcggcttc ggcgagttcg gcgtcgagct cgacggcgcg
      241 gcgctgcccg tcgcgttttg cgagccggtc gtgtcggtcg ccttcggcgc gggcgtcgtc
      301 gacggcgtca ccgaggtgtc cgtcttcggc gtcgtggtct tcggcgcggg cggcgtcgtg
      361 gttttgggcg cggtgcccgg cgtgttcggc atgccgatgc cggcgctgcc cgtgcgtgtt
      421 ggatcgacga cgctggagtt gccagctgcc gtgccgcccg gggtcttcgt cgccggaggc
      481 gtcgtcgccg tcccgctcgt cgtgcccgac gcgggcgtgg gcgtcgtcgt ggtcgtcgtc
      541 gtcggtgtcg tcgtcgcgct accggtcgtc gcgctgcccg tcgccgcgct gcccgtcgac
      601 ggtgtgtccg tcggcgtcgg cgtggtcgcc ggcagatcgg gcgtgtctgc gccggggagg
      661 cccatcgatg atgcgccgcc gctgccgagc gatgcgccgc tgccacctgc gccggagcct
      721 tcgaggagcg ccttctcttt ggcttcttcc ttcttctttg cgaggtcggc ttgcgagcgg
      781 ccgaacttct cgagcgcgtc gctcgagtcg tgcgatgcga ggaacgcgag cacgatcgac
      841 gtcgacatga agatcgttgc ggcgaccgcc gtcatcttgc gcaggaacgt cgacgcgccg
      901 gagccaccga acacggtgcc agcgttcgag ccgccgaacg cgccgcccat gccgccgcct
      961 ttgcccgact gcagcaagat cgtcaggatc aagaagagcg agacgatcga gtgcagaatg
     1021 gtgatcaggg tcgacatacc ggggagtgag ctttagccca ggggaccttt gggtgcaacc
     1081 cctaagcggg tcgtgcggcc ttcacgatcg caacaaaatc agcggcttcg agcgatgcgc
     1141 cgcctacgag cgcgccgtcg atgtcgggct cggccatgag ggcctctgcg ttgtttgctt
     1201 tgaccgagcc tccgtactgg atgcggatgg aattggccgt gtcaacgccc caccgatcgg
     1261 ccaggcgctt gcgaatgtgc gcgtggactt cttgggcttg cgcgggcgtg gcggtgcgac
     1321 ccgtgccgat cgcccacacc ggctcgtacg cgatcacgag gcgctgcgcc gcgacctgcg
     1381 cgtcgatgcc ggacaggccg ccttcgagct gcgcgtcgac gacttcgagt gtgcggccgc
     1441 tatcgcgttc cgcgagcatc tcgccgacgc acacgatcgc gccgaggccg gcaccgagca
     1501 ccgcgcgcgc tttgcgaccg acgctgtcgt tgttgtcgcc gaagaattgg cgacgttcgc
     1561 tgtgaccgac gattgtccag gtcgcgcccg cgtcggcgag gaagtgggcc gagacttcgc
     1621 ctgtgaacgc gccggtcgac tcccagaagc agtcttggct gcaggtgacg atgctcgagc
     1681 cttcgaggcg tttggcgacg ggttggatcg cgacgaacgt cggggccacg ccgaccatga
     1741 cgcctttgac ggcgccgagc tgattcttga gctcggtgac gagcgcgaga gcttcagcga
     1801 tcgtcttgtt gagcttccag ttgccgacca caaacggggt acgcgacatg gagccgggtt
     1861 tacaccgact ccatcgcgtt tgtccgggag tccgccgtca ctcggacagc cgtccgtgct
     1921 gcggccattt tctcgacgaa gatcgcaatc gatcgcgacg ttgcgcgagg cacacaaagt
     1981 gcttgggcga ttggacacga aggagatagc ctatgtctga tccgatggac ccgctcgatc
     2041 aacccgtgac gcttcgaatg ctcaacgcgg cgttggatgc atccgagcag cgcctcgcgc
     2101 agcgcctcga acaacgcctc gatcaacgcc tcgatcaacg cctcgacgcg ttcgagaagc
     2161 gcgtcgatgc ccgcatcgat gacacgctga agcgcggcct ggatgctggc ttcgagcgaa
     2221 tggaggcgct gttcggcgtc gttgacgaca agtacaagca catcccgccg aaggttgccg
     2281 tgctcgaaga gctcgagccg cgcgtcgcgc gcctagagcg cgcggtgttc gcgccaaagc
     2341 cgaagcgccg ccgcaagagc gcctaaccta gccgcgcgac cacgtaagct tgaactcgca
     2401 tgcggtttga cccgcagttt ggcagcgcgg atgcgaggcg ctgacttgct cggcgcctgt
     2461 tagctcgacg atgcgctcga gctccgcacc gacgagcgcg cacacgtgcg gcgagccggg
     2521 aaacccgtgc agcctgacct cggcgccgtt gtgcacggcg acttcgacct cgcaccaatc
     2581 gtggtagcga tcccagaacg tcggcagcgc ggcgaggacc gtttcgggag agagcgacgt
     2641 ggggtcggcg ccgaacaggc gcgcgaatgt cgcactcatc gtgccgcggc cgactttgcg
     2701 tggaacgagc gcgttgggca gcacgccgat cgcgcaatcg agcgcggtga ccagctcgga
     2761 gagctcgacc catgcaagcg gtgcgagcgt cgatgtgagc gcgttcgcga gctcgggatg
     2821 ctcgttcgtg atccgcgcca tgcctgattc gccgatgtgg tgttgcagga ctttcgagag
     2881 gacgcgcagg tgagcggcgc gcacgcggcc tttgatctcc ggcctgagcg cggagagcgc
     2941 gacgacgttg ctcgcctctt cgatgccttg cgtcggaccg aggaccgact ccgcttgcag
     3001 cgcggcgaca tcggcgtcgg acaccggcac acggatctcg ccgctcatgc gttcgagtgc
     3061 acggccaagc gcgatcgcga aggtcgacgc ggacgcccac cgcttcttgg gatttggcag
     3121 cagcgctttg cggaacacca cgtcgacggc ctcggacagg ccgtgacgca gcagcgatgg
     3181 cgggcccatc ggatcgttga gctgacgctg cacgacttgt ggcgcggggc ccgagccaaa
     3241 tggcgggcgc ccggtgagcg cgcaatacat cgtcgacgcg aggccgtaga cgtcggtctg
     3301 cgggccgcct tcttgctcga ggaatgattc gggcgcggcg aagccgggtg tgccggcggc
     3361 gtcggattga ttgccggctt tgacggcgac gccaacgtcg acgagcaccg cgcgatcgcg
     3421 ctcgcgatcg agcaggatgt tggcgggctt gacgtcgcga tggatgaggc caagcgcgtg
     3481 catcgagtcg agcgcatcgc cgatctcgag ggcgatctgc gcgacggctt cggtcgggaa
     3541 ccactcgcgg cgttcgagcg tggcgcgcag gacttcggag agtggctggc cttcgacgag
     3601 ctccatcacg aagtagacct cgcccgcgtg ctcgccgagc gcgtagacct gcacgaggtt
     3661 cttgtgatgc agcgacgcga ggatgcctgc ttccgcgcgg aatcgatcga cgagatcgcg
     3721 atcgctcgcg aggtcggagc gcagcacttt gatcgcgacg gggcgaccga ggccgagatc
     3781 ctcgccgcga tagacaacgc ccatcgcgcc gcgcgacagc tcgtggacga cgcgatacgt
     3841 gccgccgacg gtggtgccgg ccgagagcga cccggtgtcg gcgtgaacgt cgcgatcttg
     3901 aatcgcgcga tcgtcgggag agatgatgcg aacgtcgccg atcggtgcgg tgcgcagcgc
     3961 gccgtagcag cgcgcgagtg cggcttcgcc ggagcgttcg ccaacgccga tttcggtgag
     4021 cacggcgcgg acctgcacgc caagcgtcgt ctcgtcgtcg atcttgaccg gctcgttctg
     4081 gaccttgaac agcagcttgc gcgcgacgag gacggcctga tcctcgtttg caccgacgag
     4141 gaggacggcg agctcgtcac caccggggcg gccgatcgaa tcgttgccgc gaacggtcgc
     4201 acgcacgcgc gccgcgattt gcgcgagcac ttcgtcgccg gccttgtgac cgtgctgcaa
     4261 gttgatgcga cgcagcccga cgacatcgaa cagcgcgagc gtcagcttct cgttgcgccg
     4321 gccggccgcc gcgacctcgt gcgtgatcgt ttgctcgaac gcgccgcgcg tcatcgcgcc
     4381 ggagagcgga tcgtgcagcg acgtcgcgag caagcgctcg ccttcagcga cgatgcggcg
     4441 gtggctcgtc agccatgaca gctcgcgcgt gagccgcgcg gcgatcgctt tgagatcggt
     4501 gcgctcggcg gcggagaagc ggcggcccga atcggcaacg atcgcgacga ggcctgcgac
     4561 ttccgttgga ccggagcgca gcgcatcggc gatcacggag cgaggctgcg ggccgccgac
     4621 gatcacggtg ccgcccgatg cagcggcgat acgcgatgcg gcggcgatcg tcgcgcgata
     4681 gccatcgacg ggaggcgggc gcgacgcggt cggcgtcatc tgtgcgccgt ctttccacca
     4741 cacgatgcaa tcgttggccg caaagccttc ggccacactc cgcagcgtgt cggacgcagc
     4801 gcctgtgaga tcgacgagcg atgtcgcgat gagcccgcgt gcgcgagcga gatcgagctc
     4861 ggctgcaacg gtctctggtt gcgcgatcgc ggcgagcgcc tcgggcgaca ccggcgtgag
     4921 cagaacgccc atcgcgctcg cactcgcggc ggcgccggcg acacgcgcgg cattcgtacc
     4981 gtctcctacg atccagcggg gcgcggctcc gtccggcggc ggaccgagat cgctcgcgac
     5041 ggcgggtgcg acgagcagcg cggcgaggct attcgtcctg gtttcgggcg aatcgacgtg
     5101 attgagctcg gcatgcgcac gatctgcgac ctccgcgagg tcgtcgatta gacggccgga
     5161 cgggtcgaag acccccaggc gcttcgctgc cacgaatcca tcatatcatc tgggttgaat
     5221 ggccgagcgc acgggggacg cagacgcgac tgaagcggat gcagtggacg cgccgccgac
     5281 cgaggcgacc aagcgcgagg ggcacttgca ttccgatcgc ggcgcgaggc gctccgaccc
     5341 cgagtacatg acgcggggaa cgctgatcgg tcgctatgtt gtcgtcgaga agctcggcga
     5401 gggcggcatg ggcgtcgtgt attcggccta tgaccccgag ctcgaccgca aggtcgcgat
     5461 caagctcctg caggctcgcc cgggtgggtc ggaatcggcc ggcggccagg catggctcct
     5521 ccgcgaggcg caggccttgg cgcggctcca gcacccaaac gtcgtcggcg tccacgatgt
     5581 gggcacgttg cccggcgatc gggtgttcgt cgcgatggag ctcgtcgagg gcaagacgct
     5641 gcggaaatgg ctgaaagccg agaagcggtc atggcgtgcg ctcgtgccgg tgctcgtcga
     5701 ggcgggcaaa ggcctcgtcg ctgcgcatgc cgcaggtctc gtgcaccgcg acttcaagcc
     5761 ggacaacgtg ctcgtcggcg acgacggtcg cgtgcgcgtg atggacttcg gcctcgcgcg
     5821 gctgcggcgc ggcgccgaca ccggaccgat cccacgtgtg tcggcgaccg atcttctggt
     5881 cgacgcgaag agcccgctga ccgacagcct gacgatggtc ggctcggtga tcggcacacc
     5941 ggcctatatg gcgcccgagg tctacgacgg cacgcccgcc gacgcggtga ccgatcaatt
     6001 cgcgttcggc gtgacggcgt tcgaggcgct gttcggctcg cggccgttca cgaaagagca
     6061 gctcacgccg ccgcgcgctg aggtcccaaa gccaaagctg cccgaccaca cgaacgtgcc
     6121 agcgcggatt caacgcgcgg tgctgcgcgc agtcgcgatc gatcccgcgc agcgtttccc
     6181 gtcggtcgaa gcattcgtcg ccgagctgca agtcgatccg atgaagggcc gcaagcgcgc
     6241 gctcatcgcg ggtggcgtgc tggccacggc gatcggcggc ttcgcgatct cgctcgcgtt
     6301 caagaacgac gcaccgccgc cgtgcaccgg catcgaggat cagctcgcgg aggcgtggag
     6361 cccctccgtc aaggagaagg tgaaagcggg cttcgtcgcg acgaagaagc cgctcgccga
     6421 ggcgaactac aacctggttg cgcaagcgct cgatcgctac gcagccgaat ggacgcagac
     6481 cgcgatcggc agctgcaagg cgacgcgcgt gacgaagaac cagaccgagg ctgtcctcca
     6541 gcttcgccag ggttgcctcg atcagcgccg tcacgagctg aaggcgctcg ccgcggtcct
     6601 cgcggaacca agcgacgtgc tcgtcgataa agcagacaaa gcggtgttcg agctcgagcc
     6661 cgtcgcgtcg tgcaatgacg tcgaggttct ggctgcgccc ggcgatccgc caaaggacgt
     6721 cgtgcccaag ctcgagaagc tcggcgagat gctggctctg gcacgcgcgc aggtcatcgg
     6781 cgagcagatt tttccggcga tggtgagcag cgataaggcg ctcaaagaag cgcgcaccgt
     6841 cggttggccg ccggcggtcg ccgaagcgct gctcgtgcgt gcctctgcga tgtcgcggac
     6901 gggcaactca caggaagcgg cggcaatgca gctcgaagcg acgtggatgg cgatccgcgc
     6961 gcggcgtgac gacctgacgg cgtcgtcggc attgtcggct gcgatgcagg tgtccgacgc
     7021 gctcgggcga ccgggcgagg caaagatcta cgtgcagctc gccgaggagt tcgcgaagcg
     7081 cgccggtctc gacaagcgcc tcgagccgcg catcaactcg gtgcgcggaa tggtcgcggc
     7141 gcagagcggt gacctgctcg gcgcggtcga tgcgcaccaa aaatcattcg aggcggcgca
     7201 gcagctcctc ggtgccgaga gcccggcgct gtggagcgac gagtacatgt acgcgacgac
     7261 gctcacgacg gccctgtcgt acgagcgcgc cgtcggccac tacgagcatg cgatgaagct
     7321 tcgcaagcag acggtcggcg ccgaacacat cgacatcgcg ctcctcgagt cgaacctcgg
     7381 cacgtgttac gggcacctcg gccaggtcga caaggcgatc gcagcgtttg atcgaacgat
     7441 cgcgattcgc cagaagctgt tcggcaagaa caacccgctc ctcgtcgtgc cgctcgtgaa
     7501 ctacgcggac atcctcaacc gcgagcacca agcagagaag gcgctgccgc tgatcgaacg
     7561 ggcggtcgca ctctcagtgc cactgcccgg cgtcgagcac ccgacgtacc accaagcggc
     7621 gacgacgcgc gccgagatcc tcgcgagcct cggccgcatt tcggaagcgc gcaagctgac
     7681 cgaggacgtc atggcgatcg agacgcgcct gaaatcgccg acgctgccga cgacccaagc
     7741 gtcgcgcgcg gagatcgagc ttgccgcgaa ggagtacgcg accgcggcga cctacgccga
     7801 acaatccatt gcgggcttcg aagcggcgag tggcgtggac aatcccgagc tgtggcggcc
     7861 gctcaccctg ctcgcaaagg cgaagctcgg cgccggcgac aacgccgccg caaagcccct
     7921 gctcgagcgc gcggtcgcga ttgccgcgaa ggccggtctc agcgagcacg atctcgtcga
     7981 gacgaagtcg ctgctcgcgt cgctacccta agggagccgg cggcggcgac accgggagac
     8041 gaaccgtaaa cgtcgtcgtc tctggattcg acgttactgc gatgtcgccc tggtgggcgc
     8101 gcacgatctc gcgggcgata tagaggccaa ggccaaggcc actgccgccg ctgcgtttgg
     8161 tctggcggaa cggcgtgaac agcaccgcct gctgctcgtt cgtgagcgcg accttgctcg
     8221 cgttgcacac gcgcaacgca gcgacgtcgt cgatctgctt cgtctcgatg acgacggggc
     8281 tgcctggcgc gccgtgaatg atcgcgttgc cgacgaggtt cgtgaccacc tgagcgaggc
     8341 gctcgcgatc ccacatgccg cgcacgttgt cgagccgtgg ttcgaacttg gcttgcggat
     8401 acgcgaccgt gagctcgtcg agcacgtcga cgacgatgct gccgaggttc acttctgcgg
     8461 ggtgcagcgg gatgccgccg ccgagctgcg tgcgcacgac atcgaggagc tgctcgatga
     8521 tgccctgcat gcggctcgtc gcaattccca tgcggtccag cgtgacgctg accttcgcgt
     8581 tgtcggggga gaggagcttg atcgtctccg cacccatcat gagcgtctgc agtggcgaac
     8641 gtagatcatg gctcacgatg ccgagcacgt agcgttggaa ctcggccgcg cgctctcgct
     8701 cgaggattgc ttggcgctcc gccgtgatgt cgtcgaacgc gacgatcgcg gacgtcacat
     8761 tgttgcgaag gtcgcgaagc ggcgtcgcgg agagccggat gatcttcgtg tcgcgctcgg
     8821 agacgttgag atcgagctcg acgccacggg tgacctcgcc gcgcagtgcg cgcagcatcg
     8881 gcgtctccga cggagcgatc gcctcgccgg tctgcgtgcg gagcgagaca tgagccgcga
     8941 ggtccgacag cgcgcgcatg gtccgcagct gcgggaggtg gtcgagtgcg atctggttga
     9001 tgtgggtgat cgagccggct tgatcggcga cgaggatgct gatcggcagg tgctcgagaa
     9061 tcgtctgcag ctgcatgcgc gtctcgcgct ccgcctcgga acgcagcgcg ttctcgaatg
     9121 cgatcgccgc gtattcgagg cacgtctgga tcgtgacgag atcttcctcg gagaacggca
     9181 ccgagtggcc gtcgcgcatc gcggtgacga cgcccgtcac tggacttccc gatgggaacg
     9241 gaacgaccgc gagcgccgtc atgccgaagt ggtcgatcgc ggcgtgatag gccgcgggaa
     9301 gcttcgcgcg cgcctctgcg ctgctcgcga acacgtagct gcacgggtcc atcgcgctga
     9361 acgtcgtgag cagctgctcg tcgcggcgcg tgcgatgccg cgcgagcgtg aggccgggca
     9421 atgcgccggt gatcagcgtg atcgtgcagc catcgcagat cccatcgatg agattcgtcg
     9481 cgagcgcttg cacgattgcg gtcggacgcg ggccgatgtc gatgaacgca cggccgagct
     9541 tgccgatgac cgccaggcgc actctctcgc gggcggccgc ttcgcgcatc tgcttcgcgc
     9601 ggatcgcatt gtcgacgcga gcgcgcagct cctcgggcac aaacgggcga ggaatgtaat
     9661 cgttggcgcc gatcgacaga ccttcgacga cgtcgcgagt ctcgatgcgc gatgcggtga
     9721 cgatgatgat cggaagatcg cgcgtctgcg gctgcgatcg aaggaaccgg cacacttcgt
     9781 cgccgctgac cgacggcatc acccagtcga ggaggagcac atcgggctgc cgacctcctt
     9841 tcgacaaccg ctcgatgact gccgagccat cattgaacag ctcgaagtcg tacccggtgc
     9901 cgagcgagcg ctgggtgtac atcgcctcca tggcgctgtc gtccgtgatc cagacgagtg
     9961 gtcggtagtc agtcggctgt gacatggcga ataatctcgc gtggtatgta gtcgagagct
    10021 gccgcgcgcg cgccgatacc ggcttcgaag acaacgcgtg gcattccata gacgacgcag
    10081 ctggctggag cctcggtgat caagctaccg cctgcttgcg cgatggctcg cgagccgatg
    10141 aggccatcgt cacccatgcc cgtcagaacg acgccgaggg cgccagagcc ggctgcttcg
    10201 gcaccgctcg taaacaattg atcgaccgca gggacgtgcg gcgagatcgg caacggatcg
    10261 agatgaccga cgaaaccgga cccgaggcgg cggatcttga ggtgctggcc cgcacgcgcg
    10321 agcacggcgc gaccgggcac gagctcgacg ccgtgatgcg cttcgaccac ctcgagcttc
    10381 gacacgccgt ccagccgctt cgcgagcgct tcggtgtagc cgatcgggat atgcagcacc
    10441 atggcgaccg gcgacgcgag atcaccgggc agctccgcca ggaggcgcgt caacgcctgc
    10501 gggccgcccg tcgacgttcc gatcagaacg aggtctgcgc gctggacgcg ggcgagggca
    10561 gccatcggcg actgcagctc gttg
//
LOCUS       DATLBZ010000172         9064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_166216, whole genome shotgun sequence.
ACCESSION   DATLBZ010000172 DATLBZ010000000
VERSION     DATLBZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166216"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VMZ53_09065"
     CDS             complement(<1..306)
                     /locus_tag="VMZ53_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28648.1"
                     /translation="MTTTRQHGWASRSCIPRVSLWIALGVAFTAAACTDFSTPDRGVC
                     GNGLLEPGEDCDSSDATCVKCAVACSTSAQCPSSAYACGVDGFCHAPGGELDDLHGAG
                     "
     gene            complement(303..1901)
                     /locus_tag="VMZ53_09070"
     CDS             complement(303..1901)
                     /locus_tag="VMZ53_09070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28649.1"
                     /translation="MPDSSAPSAPTLPTSLDADLEPGQEVGEYQIENKLGQGAFGTVY
                     KAAHPLIGKVVAIKVLATKLSVDPEMVSRFIAEARAVNQIRQRNIIDIFSFGKLADGR
                     QYYVMEYLDGEPLDALLDRVGNLSLADALPILRPIAKALDAAHAKGIAHRDLKAENIF
                     LGTESDGVLFPKLLDFGIAKLMGNEETLKHKTRTGSPIGTPYYMSPEQCLGKDVDHRT
                     DIYAFGILVFKMLTGAYPFDADDYMSILMQQLNADAPAPSTHLPDLPASIDDVVLWLL
                     AKDPAQRPPDLRTAVKALEHAAQEAGLDIGRIPSDWDVQTSPSGKPFQTQPPPRRTNV
                     VTAPAKPALTNPSRAVTTGAGAARAKSSRIFIAATALASLALIAAIVFVVAKKSPSSS
                     QNARPTGGHEVAEVAPVPSRTPDATPDAGQQVAEVASVPNRTPDATAAVIVTVAGVPD
                     GTEVLVAGKVIGAAPGPVQLERGTAAVVLTFRADGYLPTSKSVVPDGDKLLDAALKKK
                     PKVSGGGHRPGRDDIIDVFGGGKK"
     gene            complement(2035..4257)
                     /gene="gspD"
                     /locus_tag="VMZ53_09075"
     CDS             complement(2035..4257)
                     /gene="gspD"
                     /locus_tag="VMZ53_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HUS28650.1"
                     /translation="MRALCVVLVMVVMANVARAESELPTPAASADESLYSCKQRQGDV
                     EITFKSEMELKDLLTWVVGFTCKKFVLDPKVVMLGRKVTLIAPGKLSAPQAYDVFLTA
                     LGTMGFTVVPKGKLLMVTESATSKGQTLPYYDGKLPPDSEQMVRYLLRPQFANAEAVK
                     AAMSAFKSDAGDVQVIGSMVLVTDHGANVHDMAGIAKIVDVPGGADGVYAVPVHNADA
                     TKLRDKLSDLLDLMASTKAGAGGAARDTTLTPTKLVVDERTNTLLVTGTQAAYQRVQA
                     LVERLDISLEIEGGTSFHVYQVGSAVSDELAKTLNDAISAQTTTGGEKKPVGGGSDMT
                     QLQGPVRVISDAPTNKLLVMSSGRDFLALRDVIRELDVPKRQVYIEAMILEVQTGNGL
                     QVGTSSHGGYNFGGNNSVLVGGVQAPNLRTTSLDSLLSANGLIGGLIGKTLEGSKELL
                     GQSIPSYAVLFQALADTSKTNILSTMPIIVVDNGQAKYKVGTNVPYKKGVLPTTSSAN
                     SQTTTNIEREPLLLELEIKPHISTDDSVLLDVKQQSKDIGEMNAELGPTWTERGVETR
                     VLVKDQQTIVIGGLMQVRDTSSVTKVPLLGDIPLLGHLFKYTTTSKKKMNLLIMLTPY
                     IIKDNLDMEAIRARKQREYEEFLGAVNTLDGKEYMPRIDYRRKRGLVEEINRAVQDVE
                     EEAAARAAIRKPAQIAPGLVEPKASASAGDDAKSTNPTAPVEAATPPGATVAQPAPSK
                     "
     gene            complement(4394..4807)
                     /locus_tag="VMZ53_09080"
     CDS             complement(4394..4807)
                     /locus_tag="VMZ53_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HUS28651.1"
                     /translation="MVTICDTGPLVAFLDRREKHHEWAVEMMRQVKSGPLWTCEAVLA
                     EAFYFLREFELDVEPLFQMLERGAIRVDFSLDDSWPRVRTLMGRYRQMDLADASIVVM
                     TERRKRCQVLTLDRRDFTVYRRNDRQVIPFIAPPK"
     gene            5215..5421
                     /locus_tag="VMZ53_09085"
     CDS             5215..5421
                     /locus_tag="VMZ53_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28652.1"
                     /translation="MSIGAAFIVGVLALFAMIIALLTFAQFRSVAPNAFRCARCNAEF
                     RKPAHHDFPSRCPSCGERDWSTTR"
     gene            5546..5860
                     /locus_tag="VMZ53_09090"
     CDS             5546..5860
                     /locus_tag="VMZ53_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28653.1"
                     /translation="MADTNGATDQILTTQDSVLANQARIMSYAERVEQMIANQKAILA
                     NQKTIIANQKTIAGNQKKLDQVIANQKKLDQVIANQRAIQGNQKKLDQVLANQKRILA
                     KK"
     gene            complement(5872..6243)
                     /locus_tag="VMZ53_09095"
     CDS             complement(5872..6243)
                     /locus_tag="VMZ53_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28654.1"
                     /translation="MKHLLPLLAAVAAGACADAPPHDVTVTWSLNDVTVGNYCTRVSV
                     SIGHDARPVEEHTLACDATSDVITVDDGEAATIEVHYMYWEIPAGCESQSCWHETWFA
                     SGYGTYDGASDQMAITVHRSF"
     gene            complement(6392..8077)
                     /locus_tag="VMZ53_09100"
     CDS             complement(6392..8077)
                     /locus_tag="VMZ53_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28655.1"
                     /translation="MYPAQGAPQAQQSYAQPPQQSYGQAPQQSYGQAPQQSYGQPPQQ
                     SYGQPQQGQQPQMQQPPHMQQQPHMQQQPYGQPPQQAHAQPSAATPRASGKSSKGLFI
                     GIGALVVVGGIVGIVMATRGGGGGGVDSRDGLVKATVAALSKGDADTLVKLGDPAALA
                     KFMECTKEPGESGSESEVDSQTKELREMANDVVSRTKGLKIEIAKIDERPAKLDENGK
                     NRHAFEKGQKLGSRCTAKVAMVQHKVDVTVKLGSAGETQLRLEMLEADGKWYLIDDID
                     IDVPAAAIAAGSGSVGSGSVASGSAASAAVVGGAGTATAVTPAGGGGGGYAAMLATMG
                     SLKDRMCACKDQACLDAVQADMTKWAEDQTKATPDMSDYTEAGAKQLSDSMQAYSKCM
                     TDVMMAAAGGTASATSPATTETTPPPTTATTDTTTATATATATAAAPAFVVGDRVMAQ
                     WTNGQWYAGKISAVTADGKYDVAYDDGDRSKALPASKVRKAKAGSSSGTRTSSSSSSA
                     DAPCPGPGITRRCGGRCVNIQEDDNNCGGCGNVCASGKHCDGHLFCRDAEGNL"
     gene            complement(8254..>9064)
                     /locus_tag="VMZ53_09105"
     CDS             complement(8254..>9064)
                     /locus_tag="VMZ53_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28656.1"
                     /translation="PKDHAIICEIDGVVHFGKDTKGKRKVLIVPEVGDPAEYLIPKGK
                     HLSVREGDRVRAGEALMDGPANPHDILKVLGERALAKYLVDEIQEVYRLQGVRINDKH
                     IEVIVRQMLRRVSVSDPGDTNFLVEEHVEKHIFEEENERVIVAGGQPAKGDALLLGIT
                     KASLSTESFISASSFQETTKVLTEASINGRVDYLRGLKENVIMGRLIPAGTGLGAYKR
                     LTVDVDAVDEDDIEEDLDNDGTYSSHTGSAAMAMGGGDVIDSPTGSDAVID"
BASE COUNT         1458 a         3101 c         2816 g         1689 t
ORIGIN      
        1 gccagcgcca tgcaggtcgt ccagctctcc gccgggcgcg tgacaaaacc cgtcgacgcc
       61 gcacgcgtac gcactcgacg gacattgcgc gctcgtcgag cacgcaaccg cgcacttcac
      121 gcacgtcgcg tcgctcgagt cgcagtcctc gccaggctcg agcagcccat ttccgcagac
      181 gccgcggtcc ggcgtcgaga agtcggtgca cgcggccgcg gtaaacgcga cgccgagcgc
      241 aatccataag ctaacgcgag gaatgcaact gcgtgatgcc cacccatgct ggcgtgtcgt
      301 cgtcatttct tccctccgcc aaacacatcg atgatgtcgt cgcgaccagg ccggtgcccg
      361 ccgccgctca ccttcggctt cttcttgagc gccgcgtcga gcagcttgtc gccatcgggc
      421 acgacgctct tcgacgtcgg caaatacccg tcggcgcgaa atgtcagcac gacagccgcg
      481 gtgccgcgct cgagctgcac tgggccgggc gcggcgccga tcaccttgcc ggcaacgaga
      541 acctctgtgc cgtccggcac gccggccacg gtgacgatca cggccgcggt tgcgtccggt
      601 gtccggttag ggacggacgc aacttccgca acctgctgtc cggcatccgg tgttgcgtcc
      661 ggtgtccgac tagggacggg cgcaacttcc gcaacctcgt gtccgcctgt cggccgggcg
      721 ttctgcgacg agctcggaga cttcttcgcg acgacgaaca cgatcgcggc gatgagcgcg
      781 agcgatgcga gggcagtcgc ggcgatgaag atgcgcgacg atttcgcgcg cgcggcgcca
      841 gcgccggtcg tcacggcacg cgacgggttc gtgagcgcgg gcttcgcggg cgcggtgacg
      901 acattcgtgc ggcgcggcgg cggttgcgtc tggaatggct tgccgctcgg cgaggtctgc
      961 acatcccagt ccgacgggat gcggccgatg tcgaggccgg cttcttgcgc cgcgtgctcg
     1021 agcgctttga ccgcggtccg caagtcgggc gggcgctgcg ctgggtcttt ggcgagcagc
     1081 cagaggacca cgtcgtcgat cgaggctggc agatcgggca agtgcgtcga cggcgccggc
     1141 gcgtccgcgt tgagctgctg catcaggatc gacatgtagt cgtccgcatc gaacggatac
     1201 gcgcccgtca gcatcttgaa gacgaggatc ccgaacgcgt agatatccgt gcggtgatcg
     1261 acgtctttgc cgaggcactg ctcggggctc atgtaatacg gcgtgccgat cggcgagccg
     1321 gtacgcgtct tgtgcttgag cgtctcctcg ttgcccatca gcttcgcgat gccgaagtcg
     1381 aggagcttgg ggaacagcac gccgtcggac tccgtaccga ggaagatgtt ctcggctttg
     1441 agatcgcgat gcgcgatgcc cttagcgtgg gcggcgtcga gcgcctttgc gattggccga
     1501 aggatcggca gcgcgtcggc cagtgacaga ttgccaacgc gatcgaggag cgcatcgagc
     1561 ggctcgccgt cgaggtactc catcacgtag tactggcgac cgtccgcgag cttgccgaac
     1621 gagaagatgt cgatgatgtt gcgctggcgg atctggttga ccgcgcgggc ctccgcgatg
     1681 aagcgcgaga ccatctccgg atcgactgag agcttggtcg cgagcacttt gatcgcgacg
     1741 accttgccga tcagcgggtg cgccgctttg tagaccgtgc cgaaggcgcc ctgccccagc
     1801 ttgttctcga tctggtactc gccaacctct tggccaggct ccagatcggc atcgagcgat
     1861 gtcggtaatg taggtgcgga cggtgcagac gagtcaggca taggagggcc ctctcgagcc
     1921 cggcccctac tcgtgcaaag ggttcgcccg cgcagactgt ggagcaagcc tacgtggtgt
     1981 gatcggctga ttacgcggca gtcgcctaca cgagcgccgg caatccgttg gcaatcactt
     2041 acttggcgcg ggctgcgcca cggtggcgcc cggcggtgtt gccgcttcca ccggtgctgt
     2101 ggggttcgtc gactttgcgt cgtcgccggc cgacgccgac gccttcggtt cgacgaggcc
     2161 cggcgcgatc tgcgcgggct tgcgaatcgc tgcgcgcgca gccgcttcct cctcgacgtc
     2221 ctgcaccgcg cggttgatct cttcgacgag gccacgcttc cggcggtagt cgatgcgagg
     2281 catgtattcc ttgccatcga gcgtattcac cgcgccgagg aactcttcgt actcgcgctg
     2341 cttgcgagcg cgaatcgcct ccatatcgag gttgtccttg atgatgtacg gcgtgagcat
     2401 gatgagcaga ttcatcttct tcttgctcgt cgtcgtgtac ttgaagagat gaccgagcag
     2461 tgggatgtcg ccgagcagcg gcaccttcgt cacgctcgac gtgtcgcgca cctgcatgag
     2521 gccgccgatc acgatcgtct gctgatcctt cacgagcacg cgcgtctcga caccgcgctc
     2581 cgtccacgtc gggccgagct ccgcgttcat ctcgccgatg tccttgctct gctgcttcac
     2641 atcgagcagc accgaatcgt ccgtggagat gtgcggcttg atctcgagct ctagcagcag
     2701 cggctcgcgc tcgatgttcg tcgtggtctg cgagttcgcg ctcgacgtcg tcggcagcac
     2761 gcccttcttg tatggaacgt tcgtgccgac cttgtacttc gcctggccgt tatcgacgac
     2821 gatgatcggc atcgtcgaga ggatgttcgt cttcgacgta tcggcgagcg cctgaaacag
     2881 caccgcgtag ctcgggatgc tctgaccgag gagctccttg ctgccctcca gcgtcttccc
     2941 gatgagcccg ccgatgagac cattcgcaga cagcagagaa tcgagcgacg tcgtcctcag
     3001 gtttggcgcc tgcacgccac cgaccagcac cgagttattg ccgccgaagt tgtagccgcc
     3061 gtgcgagctc gtgccgacct gcagcccgtt gccggtctgg acctcgagga tcatcgcctc
     3121 gatgtagacc tggcgcttcg gtacgtcgag ctcgcgaatc acgtcgcgca acgccaagaa
     3181 atcgcgaccc gatgacatca cgagcagctt gttcgtcggc gcgtcgctga tcacgcgcac
     3241 gggcccctgc agctgcgtca tgtcgctgcc accgcccacg ggcttcttct cgccgcccgt
     3301 cgttgtctgc gccgagatgg cgtcgttgag cgtcttcgcg agctcgtcgg agacagcgct
     3361 gcccacttga tagacatgaa acgacgtccc gccctcgatc tcgagcgaga tgtcgagccg
     3421 ctcgacgagc gcttgcacgc gctgatacgc cgcctgcgtg ccggtgacga gcaacgtgtt
     3481 cgtgcgctcg tcgacgacga gcttcgtcgg cgtcagcgtc gtgtcgcgcg ccgccccgcc
     3541 cgcgccggcc ttcgtgctcg ccatgagatc gagcaagtcg ctcagcttgt cgcgcagctt
     3601 cgtcgcatct gcgttgtgaa caggcaccgc gtagacgcca tccgcgccgc cgggcacgtc
     3661 gacgatcttc gcgatgcccg ccatgtcgtg cacgttcgcg ccgtggtccg tcacgagcac
     3721 catcgagccg atgacctgca cgtcgcccgc gtcggacttg aacgcgctca tcgccgcctt
     3781 caccgcctct gcgttcgcga attgcgggcg cagcagatac cgcaccattt gctcgctgtc
     3841 gggcggcagc ttgccgtcgt agtacggcag cgtctgccct ttactcgtcg cggattccgt
     3901 caccatgagc aacttgccct tcgggacgac ggtgaagccc atcgtcccga gcgccgtcag
     3961 aaagacgtcg tacgcctgcg gcgcgctcag cttgcccggc gcgatcaggg tgaccttgcg
     4021 cccgagcatc acgaccttcg gatcgaggac gaacttcttg cacgtgaagc cgacgaccca
     4081 cgtgagcaag tccttcagct ccatctcgct cttgaacgtg atctcgacgt cgccctgtcg
     4141 ctgcttgcag ctgtagagcg actcgtcggc gctggccgcg ggcgtcggca gctccgactc
     4201 ggcccgcgcc acattcgcca tcacgaccat cacgagcacg acacagagag cacgcatgcg
     4261 ctctcgcact accaagacgc gggccagcgc gcgcgacatc gataagcgcg tgtttcttcg
     4321 tcgggcccgc cggcagccgt gccagcaagt cacgacagcg ccgcgcttcg cgcgccgaat
     4381 tcacatttgc ccgttacttc ggtggcgcga tgaacggaat cacttgccgg tcattgcgac
     4441 ggtagaccgt gaagtccctg cggtcgagcg tcaggacctg gcaacgcttg cgccgctccg
     4501 tcatcaccac gatcgatgca tcggcaagat ccatctggcg atagcggccc atgagcgttc
     4561 gcactctcgg ccatgagtcg tcgaggctga agtccacgcg aatggcacca cgctctagca
     4621 tttgaaagag cggctccacg tcgagctcga attcgcgcaa gaagtagaac gcctcggcga
     4681 gcacggcttc gcacgtccag agcggccctg acttcacctg ccgcatcatc tcgaccgccc
     4741 actcgtggtg tttctcgcgg cggtctagaa acgcaacgag cgggccggtg tcgcaaatcg
     4801 tcaccatgac cggcggaact tccgacggtc gtttgtcgcg gacacacccg gtccttccaa
     4861 gcatccagcg aggtcagcca cgagctcata tacagatgac gccacgcgtg agggctgctc
     4921 ctcgaggcgc tctcttatca gggccgccac cgaacagcct cgagcgcgcg cctcgcgccg
     4981 caatcgcgag agggtcttct cgggtagctt gatggtaatt cccttcatgt cagaagggta
     5041 acaccccaca accctcctac caatccggtt ctcgacgtcc atctctcgat gcgaagcaat
     5101 gcgcacgcca gcgacggcga accacaaatc gcggccttgc cgatgcgcaa agccgtccac
     5161 cggacgcgac gtccgaatct cggcggacac cggacacgct gctaggctca gcgcatgagc
     5221 atcggcgcag cgttcatcgt cggcgtcctt gcgctgttcg cgatgatcat cgcgctgctc
     5281 acgttcgcgc agtttcgcag tgtcgcgccg aacgcgtttc gctgcgcgcg ctgcaatgcc
     5341 gagttcagga agcccgcgca tcacgacttc ccgtcgcgct gcccgtcgtg cggcgaacgc
     5401 gactggtcga cgacgcgctg acgaatcacg tgggctccca cgcaactcgc gcccgacgtc
     5461 ccgaacgatc gcatcccgaa acgcgcgccg ttcgcttgcg tcaccctcgc ccgccgcgct
     5521 tctaattgcg gtccaggagg acccgatggc ggatacgaac ggtgcaacgg accagatcct
     5581 tacgactcaa gactcggtgc tcgctaacca agcgcgcatc atgtcgtacg cagagcgggt
     5641 cgagcagatg atcgcgaatc agaaggcgat cctcgcgaat cagaagacga tcatcgcgaa
     5701 ccaaaagacg atcgcgggca accagaagaa gctcgaccag gtgatcgcga accagaagaa
     5761 gctcgaccag gtgatcgcga accagcgcgc gatccagggc aaccagaaga agctcgacca
     5821 agttctcgcg aaccagaagc gcatcctcgc gaagaagtag cgcgtggcgc gttagaagct
     5881 gcggtgcacc gtgatcgcca tctggtcgct tgcaccgtcg tacgtgccgt agccgctcgc
     5941 gaaccaggtc tcgtgccagc acgactgtga ttcgcagccc gctgggatct cccagtacat
     6001 gtagtgcacc tcgatcgtgg ccgcttcgcc atcgtcgacg gtgatgacgt cactcgtcgc
     6061 gtcgcaggcg agcgtgtgct cctctacggg cctcgcgtcg tggccgatcg atacgctgac
     6121 gcgcgtgcag tagttgccga ccgtcacgtc gttgagggac caggtgaccg tcacgtcgtg
     6181 tggcggtgcg tccgcgcagg ctcccgcggc gaccgcggca aggaggggca acaggtgctt
     6241 catgccctgc gcatcacgca agcacggcgc caacgcacgc cggtcgcaac catctgattg
     6301 cccgtcgttg tctggccagt ccattcgccg ggcgggctct acatgcgacg tagcggtgcg
     6361 ccaatgcgcc agtcgctggc tacccggttc gtcagaggtt gccctcggcg tcgcgacaga
     6421 acaggtggcc gtcgcagtgc ttgcccgaag cgcacacgtt gccgcagccg ccgcagttgt
     6481 tgtcatcttc ttgaatgttg acgcagcggc ctccgcagcg gcgcgtgatg ccaggtcccg
     6541 gacacggcgc gtcggcagag ctcgaggagc tcgacgtccg cgtgcccgac gacgatccgg
     6601 cttttgcctt gcgaaccttg ctcgcgggga gtgccttcga tcgatcgccg tcgtcgtagg
     6661 cgacgtcgta cttgccgtcc gcggtcaccg cgctgatctt gcctgcgtac cactggccgt
     6721 tcgtccactg cgccatcacg cgatcaccga cgacaaatgc gggtgcggcg gccgtcgccg
     6781 tcgccgtcgc ggtcgctgtc gtcgtgtcgg tcgtggcggt cgtcgggggc ggcgttgtct
     6841 cggtggtcgc cggcgaagtc gctgaggccg ttccgcccgc agccgccatc atgacgtcgg
     6901 tcatgcactt cgagtacgcc tgcatgctgt cgctcaactg cttggcgcca gcctcggtgt
     6961 agtcgctcat gtcgggcgtg gccttcgtct gatcctcggc ccacttcgtc atgtccgctt
     7021 gcaccgcatc gaggcacgct tggtccttgc acgcgcacat acggtccttc agcgagccca
     7081 tcgtcgcgag catcgctgcg tagccgccac cgccgccgcc cgccggcgtg accgccgtcg
     7141 ccgtgccggc tccaccgaca acggccgccg aagcagcgct gcccgaagcc acgctgccgg
     7201 aaccgacgct gccggaaccc gcggcgattg ccgctgctgg cacgtcgatg tcgatgtcgt
     7261 cgatcaagta ccacttgccg tcggcctcga gcatctcgag ccgcagctgc gtctcgcctg
     7321 cactgccgag cttcaccgtg acatcgacct tgtgctggac catggcgacc ttcgccgtgc
     7381 agcgcgagcc gagcttctgc cccttctcga acgcgtggcg attcttgccg ttctcgtcga
     7441 gcttggcggg ccgctcgtcg atcttcgcga tttcgatctt cagtcccttc gtcctcgaga
     7501 cgacgtcgtt cgccatctcg cgcagctcct tcgtttggct gtcgacctcg gactccgacc
     7561 cgctctcgcc cggctccttc gtgcactcca tgaacttcgc gagcgccgcc ggatcaccga
     7621 gcttcacgag cgtatcggca tcgcccttcg acaacgccgc gaccgtcgcc ttcacgagcc
     7681 cgtcgcgcga atccacgccg ccgcccccgc caccgcgcgt cgccatgaca atgccgacga
     7741 ttcccccgac gacgaccagc gcaccaatcc cgatgaacaa acccttcgag gattttcccg
     7801 acgcgcgcgg cgttgctgcc gagggttgag cgtgcgcctg ctgcggcggc tgcccatacg
     7861 gctgctgctg catgtgcggc tgctgctgca tgtgcggcgg ctgctgcatc tgcggctgct
     7921 gcccctgctg aggctgcccg tacgactgct gcggcggctg cccgtatgac tgctgcggcg
     7981 cctgcccgta cgactgctgc ggcgcctgcc cgtacgactg ctgcggcggc tgcgcgtacg
     8041 actgctgcgc ttgcggcgca ccctgcgccg gatacacggc gcgacaccga tcacacaccc
     8101 atgcgttctg ctcgccggcc caacgcaacg tccatccaca cgaccggcat gcctgagcat
     8161 tcaccatgct gcggacgtat cacatcgtcg acgccgaaac gacaaacggc ccgtccgaag
     8221 acgagccgtt aaacatccgc gaagcggcag tgcttagtcg atcaccgcgt ccgagccggt
     8281 cggcgagtcg atgacgtcgc cgccacccat cgccatcgcg gccgagccgg tgtggctcga
     8341 gtacgtgccg tcgttgtcca aatcctcctc gatatcgtcc tcgtccaccg cgtcgacatc
     8401 gaccgtgagg cgcttgtacg cgccgaggcc ggtgcctgcc gggatgaggc gacccatgat
     8461 gacgttctcc ttcagaccgc ggaggtaatc cacgcgcccg ttgatcgacg cctcggtgag
     8521 caccttcgtc gtctcttgga acgacgatgc cgagatgaac gactcggtgg agagcgacgc
     8581 cttcgtgatt ccgagcagga gtgcatcacc cttcgccggc tgtccgccag cgacgatcac
     8641 gcgctcgttc tcttcctcga agatgtgctt ctcgacgtgc tcctcgacca ggaagttcgt
     8701 atcgcccggg tcggagaccg acacgcggcg cagcatctgg cgaacgatca cctcgatgtg
     8761 cttgtcgttg atgcgaacgc cctggaggcg gtaaacctcc tggatctcgt cgaccaggta
     8821 cttcgcgagc gcgcgctcgc cgaggacctt gaggatgtcg tgcgggttcg ccggaccatc
     8881 catgagggct tcacccgcgc ggacgcggtc gccttcacga acggacaggt gcttgccctt
     8941 cgggatcagg tactcggccg gatcgccaac ctcgggaacg atgaggacct tgcgcttgcc
     9001 cttcgtgtcc ttgccgaagt gaacgacgcc gtcgatctcg cagatgatgg cgtggtcttt
     9061 cggc
//
LOCUS       DATLBZ010000173        12994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_167724, whole genome shotgun sequence.
ACCESSION   DATLBZ010000173 DATLBZ010000000
VERSION     DATLBZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12994
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167724"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1370
                     /locus_tag="VMZ53_09110"
     CDS             189..1370
                     /locus_tag="VMZ53_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUS28657.1"
                     /translation="MKRALVLLALLAGCGQQRENGVVLWHAYTGQERAALEVTAATWN
                     AAHADTPLVLVAVPYDSFADKLTSAIPRGNGPDLFIYPHDRIGDWADAGTIEPIEFWV
                     DDARADRFTEQAIGAMAYHGSLWGLPLSAKSLALFVRTDLVATPPTTTDALIALAPQM
                     KSKHGFALAYANVDLYFHAPWLHGFGGAVMDDEDRLSIATPEAASAMTFARRLVAEGI
                     SPADAQEPLVATLFNEGKAATVMSGPWFVAQINANVPWEVHTLPVISETGKPAAPFLG
                     AEGILMSAQAHDKQAAFAVMDALTSDAAAVVRAKVARQVVANIHAYDDPDVAKDPALK
                     AFREQLDHSVPMPKSGAMRMVWTPYKTALGEVLSGRAQAGAQLISVEKEVNSYLQGPA
                     K"
     gene            1367..3067
                     /locus_tag="VMZ53_09115"
     CDS             1367..3067
                     /locus_tag="VMZ53_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HUS28658.1"
                     /translation="MSPLQRQMRDFAIFATLALIVATTAFVAVRNTELAARQIDLAQR
                     TADALAHSRVPLPTVHGAVIGPAIPSKKHPYLRRRMILEVTGWRELGGAVGPATSGGH
                     DVRNADASSDADAVRLADDKLFYDAARRFDRTGPFAELMPDGSGRAIAAVAATDDGEV
                     QAVPGRPRPPKLPPEQRAIVAIAITQPAAPAPYPFLVIIGVLGLGAAFAAAGAIVGGR
                     AARVGMFASIAAIAVPTMLWGAWIGTTVILCLAAAVAFAQRAQLTDRIATGVFRNRTA
                     MSFLAPAAIAMFVLVAAPFVIGIVLGFYDHHHGTWTFVGLSNFKEILSGGGRSLDDPL
                     NFWFILGVTVLWTLANITLHVALGVALALLLSRKWIRARGVWRMLLILPWAIPNYITA
                     LIWNGMFQGEYGAINSLLHACGLSDVSWFSSWATAFSANVITNTWLGFPFMMVVALGA
                     LETIPKELYEAADVDGASKWQQFRHITLPHLRPALGPAIALGSIWTFNMFNVIFLVSG
                     GKPGNSTNILVTDAYRWAFERGERYGMAAAYATIIFLILLLWTVFGTRIVRSKGEAET
                     "
     gene            3064..3921
                     /locus_tag="VMZ53_09120"
     CDS             3064..3921
                     /locus_tag="VMZ53_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HUS28659.1"
                     /translation="MSTKRRPGIVASSAAYLALVVVTAVVLYPVMLVCKKAFEPGKNF
                     ALSASPIPHTTTLDNFHRLFTARGGHGELLFLRHAANSLIIAVLTTIVGVVLSCTAAY
                     ALSRFRFPGRKAGLTTFLVVQMFPATLLLLPLYVILDKLGLLNSISGLVLVYSTTAIP
                     FCVWTLKGYFDTLPRELEEAARIDGASPWMVFWKIILPLSTPGIAVTALFSFMTAWNE
                     FIMASTFMTDESKYTLPVLIQSSVGQHSADYGLFAAGAVVTSLPVMIAFYVLQKYLVG
                     GLTAGAVKG"
     gene            complement(3928..4683)
                     /locus_tag="VMZ53_09125"
     CDS             complement(3928..4683)
                     /locus_tag="VMZ53_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28660.1"
                     /translation="MATPGYPGQMGQMPPGMGGPPGMGGPPGMGGPPMGAQPMPRPMK
                     RGTSKAVPVVVSAGLAVGVFCGLLFGVGTGKSEANAAAPSASNVKKDDDAKATAAAPE
                     GLGASSTVKTPAAPSTPPTTPATGAGSATTTAATGTPQTTPPTTPTIPEAKKMKLTIK
                     IKPDAAAKDAKVTIDGKDVDGMTTEIALDKKTVKVEVKSNGYRSGEKKIDVSEGDMTL
                     EMELTKRSSGGGGVRPPKRPDRPPSSGGGLIDI"
     gene            complement(4693..5838)
                     /locus_tag="VMZ53_09130"
     CDS             complement(4693..5838)
                     /locus_tag="VMZ53_09130"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS28661.1"
                     /translation="MRLALICAFLCSSFAVASAQGEIEMEEDTSMKKPDPPPDAPPAD
                     APPADTPPADQPIVKDPKVAKKWLQAGDLLMKNGDKLAKQNKLDEAKVQYTNAVTAYQ
                     KAMEASDDVAAVPLNWQLANAEDKAGDTPSALKHMKLVVDAQGMKPDLVKKAQAKLDE
                     MSMKVGVLTLTVAPDGTQISINGKQVGEAPMTEPLVLMPGAITVSFSAVGYQPKDVEF
                     KIEAGSESERKIALEAVPIVTKPVDNDIGPEPEPDKPKQKNPMIPVYIGAGATGALLL
                     TATITGIIAVGDHATYVDPKASNSERADAQSGGRTMAHITDICLVGMIGAAAFTSYWY
                     LYKVRPQQQAMAERQAARRRPASNPSKVGVVPWVEPQAGGLVAVGSF"
     gene            complement(5851..6636)
                     /gene="recO"
                     /locus_tag="VMZ53_09135"
     CDS             complement(5851..6636)
                     /gene="recO"
                     /locus_tag="VMZ53_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HUS28662.1"
                     /translation="MAEAELGVVLKTTPLRESDLLVTLYTMTHGRVSAVARGARKSQR
                     RFAGALQLLVLGRYELGRRPRGELWGLDAAEVEREWTTLASDVFAVAHASYIAELVAA
                     LMPAESPEPHVLDVIVALWESLAEGGPSPAALRAVELELLDVAGHKPALDACAACGAT
                     DLEAGAMFDPTRGGAICKRCAATSRTAGVRPLEPATLAYLRAVAAAGSPSAARALDAD
                     PRVAPADRSAGRDAMIAMVQTLVGKPLKSLEYIAKLGAAGRRP"
     gene            complement(6640..8139)
                     /locus_tag="VMZ53_09140"
     CDS             complement(6640..8139)
                     /locus_tag="VMZ53_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS28663.1"
                     /translation="MWLRAGRAQKKLSLEDVAKITKIQARILDKLERGELDGLPAEVF
                     VKGFVRSFAKCVGLDEQEAITRYVACKSPAAALTAKALVESMGLARKAQGTPATPTPV
                     VAAQVAAVVEMMTDSPAIESPAIATPAIETPLAVTPVVIDVAPLVAAPSMIEATEATA
                     ASEPNSEAPMTLDAPVVLDSPVVLDAAPVVESMPSEQPQGKKKRSRKKNPTTGAPRTR
                     KKKNASAELASTIASTNASTNDIAPPANGIGLATTDIDDDAHTTQIVKISLDTGPVAR
                     VDAESLMTSAIEAKTDDAIFVSTTGANNANTATNANDATSDAAIAANASLEPRTSSDG
                     AVDEPRLAEGSQRISSSQIEVVDVSSLEATADSTVSDDNASEPQVSGAWQPTMPPLAS
                     TPSVPWRRPSITASRAYVVPTLVIDDADPDSADREREDRSEKDQSRLSFLPPILLDRE
                     DRSSRQGGLTLAVIILLIAATLTLSYLMRRPSPSGDGVTMIDSTSQLLS"
     gene            complement(8220..9005)
                     /locus_tag="VMZ53_09145"
     CDS             complement(8220..9005)
                     /locus_tag="VMZ53_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28664.1"
                     /translation="MLAACPGHVEHDVEKSNKRLAIANDYLAKADLEGAEGEASKAIA
                     YNPGNDEAYVIRGLVSYIRALQAKKSMEVDNCLTGVDAEAMGKDIDTFLAKADADFER
                     ATKAAPDNGEAWADRGIVHNLLEDFAVAQTYLTTALENPMRLKDPALTRANLGWSFFH
                     QNKLVEATKELLTVGQFQPKMCVATYRLGRVYFAREEWNKAAEKFQTVSDDPSCGSQE
                     ASYYLMKTQMQQGLVEDAKVSQAACLKMSLKSCIATKCRAEGP"
     gene            9120..9968
                     /locus_tag="VMZ53_09150"
     CDS             9120..9968
                     /locus_tag="VMZ53_09150"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HUS28665.1"
                     /translation="MSELPGPMTPTKTTKIETSRGGLYAEVFEPSGEPKGVALVTHGY
                     FEHCGRYRELAHVIVKAGWIALTYDVRGHGQSPGARGFVERFDEYLDDFRDAVTAAKK
                     LAPGKPLVLVGHSHGSLITLRALTSDRPPDAAAAIVSSPYLGMKVVVPGWKKGLARVA
                     SRVAPALTQPNPLKAEDLTHDKEKIAERLADKMCFEVVPVRWFTESAAAQQYVEANAS
                     RIKIPTTWLVGGADTIADPATSKRVAARVSGAHYHDLAGLKHEVFNEVERGKVFAEVT
                     KVLGAL"
     gene            10168..10953
                     /locus_tag="VMZ53_09155"
     CDS             10168..10953
                     /locus_tag="VMZ53_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28666.1"
                     /translation="MKLFACMCNQPQRLWAALAPVRAVLSVPPPVSRWGLAYSQGGDV
                     LLVRTPKSSTKPIDLSGPLAEIPTDCAIGQAVLDDGFAGTDNTPPFRFRRWMWAMAGR
                     PNIDGATPRLLEQIPEYLRRNLRGRSPGELVFHVFLAMLHDEGSVDDPNLQVHATRRA
                     LAATIKLVGAEQAKAGVEPGGLGNIAVSNGRSMVVMRLDQPLKLRKLWVNNDKNERDE
                     AFRGVLLVSGGDGDAKEGFEDVPADRAVLITRDLQVQLADLNP"
     gene            11034..11396
                     /locus_tag="VMZ53_09160"
     CDS             11034..11396
                     /locus_tag="VMZ53_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28667.1"
                     /translation="MSLDSRDNDEGSEPRRAPGVMIQRVCGSLQQLLARGVADYASDR
                     DDVIELFLEPDEIAEGGMIVISMRVDVNSPTGVRDELFSAWLAVRPNAAEGTLLTPSA
                     WLPDMVRRVYFRVRAAQR"
     gene            complement(11419..12684)
                     /locus_tag="VMZ53_09165"
     CDS             complement(11419..12684)
                     /locus_tag="VMZ53_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS28668.1"
                     /translation="MSSDILSAKFASKTLLATDRDQDIAVTITMPKGRAQVRPPLSLA
                     IVIDRSGSMEGEALANAKAAAAKLVDALSPGDAFTVIAYSTSVETVFQMSLANDANKL
                     AAARAIDSITSDGNTCISCGIEQGAAQLARSPLGGDRPYSGQGIARMVLISDGQANSG
                     LYDRDELAQLATETAAKGVSISTVGVGLDFDEVTMTRLADVGHGHYYFVEDTANLAQM
                     FRTELDSMTETVAADVRLVVRGTGMTLGEAYGYPIVARGREDIVIPVADLRAGETRKV
                     VIRSHVALNNIRLGSSYEMAQFRLQWRGVADGSVHEAQTNLVASFSDDASQVAATIDR
                     GAVNAIETARTARVLEDAARTYETQGAAAAQQVLEQHRREVQDNKYMDAPALEAIDRA
                     TSSASGNFSKAPPAKAMKATRMDAYDLAR"
BASE COUNT         2018 a         4375 c         4211 g         2390 t
ORIGIN      
        1 agtcctcggc gccgaagtgg tcctccatct cgagtccaaa gccggcccac tcacggtgcg
       61 cgccgacgcg gcctccgctc cgcgcaaagg cgacaccgtc caagtctggc tcgacccgaa
      121 atcgatccac gtcttcgacg gcgccaccga ggtccgcctg tgatcgcggc gtcgcgcgag
      181 gcgcagcgat gaaacgcgcg ctcgtgctgt tggcgttgct cgccggatgc ggccagcagc
      241 gtgagaacgg cgtcgtcctg tggcacgcat acacgggcca agagcgcgct gcgctcgaag
      301 tcaccgccgc gacgtggaac gccgcgcatg cggacacgcc gctcgtgctc gtcgcagttc
      361 cgtacgactc gttcgccgac aagctgacga gcgccatccc gcgcggcaac gggcccgacc
      421 tgttcatcta cccgcacgat cgcatcggcg attgggccga cgccgggacg atcgagccga
      481 tcgaattctg ggtcgacgac gcacgcgcgg atcgcttcac cgaacaagcg atcggcgcga
      541 tggcgtacca cggctcgctg tggggcctcc cgctctccgc gaagtcgctc gcgctgttcg
      601 tccgcaccga cctcgtcgcg actccaccga cgacgaccga cgcgctgatc gcactcgcgc
      661 cgcagatgaa gtcgaaacac ggcttcgcgc tcgcgtacgc gaacgtcgac ctctacttcc
      721 acgcgccgtg gctgcacggc ttcggcggcg cggtgatgga cgacgaagat cgcctctcga
      781 tcgcgacacc cgaagccgca agcgcgatga cgttcgcgcg ccgcctcgtc gccgagggca
      841 tctcgcccgc cgacgcgcaa gagccgctcg tcgccactct cttcaacgaa ggcaaagcgg
      901 ccaccgtcat gtccgggccg tggttcgtgg cgcagatcaa cgcgaacgtg ccgtgggaag
      961 tgcacacgct gccggtcatc tccgaaacgg gcaagccggc cgctccattc ctcggcgccg
     1021 aaggcatcct gatgtcggcg caagcgcacg acaagcaggc agcgttcgca gtgatggacg
     1081 cactgacgtc cgacgccgcc gctgtcgtgc gagcgaaggt cgcgcgccag gtcgtcgcaa
     1141 acatccacgc gtacgacgat cccgacgtgg cgaaagaccc ggcgctcaaa gcgttccgcg
     1201 agcagctcga tcactcggtg ccgatgccga agagcggcgc gatgcgcatg gtgtggacgc
     1261 cgtacaagac ggcgctcggc gaagtgctct cgggccgcgc acaggccggc gcccagctga
     1321 tctccgtcga gaaagaggtc aacagctacc tgcaggggcc cgcgaaatga gcccgctgca
     1381 gcggcagatg cgcgacttcg cgatcttcgc gacgctcgcg ctcatcgtcg cgaccacggc
     1441 gttcgtcgcc gtgcggaaca ccgagctcgc cgcgcgccaa atcgatctcg cgcaacgcac
     1501 ggcggacgcg ctcgcgcaca gccgcgtccc gcttccaacg gttcacggcg ctgtcatcgg
     1561 ccccgcgatc ccatcgaaga agcacccgta cctccgccgc cgcatgatcc tcgaggtgac
     1621 cgggtggcgc gagctgggtg gcgccgtcgg acctgcgaca tccggcgggc atgacgtccg
     1681 gaatgcggac gcttcctccg atgcggacgc tgtccgattg gcggacgaca agctcttcta
     1741 cgacgcggcc aggcggttcg accggacagg gccattcgcc gagctgatgc ccgatgggag
     1801 cggtcgcgcg attgcggctg tggcggcgac cgatgacggc gaggtgcagg cagtcccggg
     1861 tcgtccgcgg cctccgaagc ttccgcccga gcagcgcgcg attgtggcga ttgcgatcac
     1921 gcagccggcc gcgccggcgc cctacccgtt tctcgtgatt atcggcgtgc tcgggcttgg
     1981 tgcggccttt gcggcagccg gcgcgattgt cggtgggcgc gcggcgcgcg tcggcatgtt
     2041 tgcgagcatc gccgcgatcg ccgttccaac gatgctgtgg ggcgcgtgga tcggcaccac
     2101 cgtgatcttg tgcctcgccg ccgccgtcgc gttcgcacag cgcgcgcagc tcaccgaccg
     2161 gatcgcgacg ggcgtgtttc ggaatcgcac ggcgatgagc ttcttggcgc ccgccgcgat
     2221 cgcgatgttc gtcctcgtcg ccgcgccctt cgtcatcggc atcgtcttgg gcttctacga
     2281 ccatcatcac gggacgtgga ccttcgtcgg gctgtcgaac ttcaaagaga ttctttcggg
     2341 tggtgggcgc agcctcgacg atccgctgaa cttctggttc atcttgggcg tcaccgttct
     2401 gtggacgctc gccaacatca cgctgcacgt ggcgctcggc gtcgcgcttg cgctgttgct
     2461 ctctcgcaag tggatcaggg cacgcggcgt ttggcggatg ttgctgatcc tgccgtgggc
     2521 gattccgaac tacatcaccg cgctgatctg gaacggcatg ttccagggcg agtacggcgc
     2581 gatcaactcg ctgctgcatg cgtgcggcct cagcgacgtg tcgtggttca gctcgtgggc
     2641 gacagcgttt tccgcgaacg tcatcacgaa tacgtggctt ggctttccct tcatgatggt
     2701 cgtcgcgctc ggcgcgctgg agacgattcc gaaggagctc tacgaagcgg cggacgtcga
     2761 cggcgcgagc aagtggcagc agtttcgcca catcacgctg ccgcaccttc gtccggcact
     2821 cggaccggcc atcgcgctcg gctcgatctg gacgttcaac atgttcaacg tgatcttcct
     2881 cgtgtccggc ggtaagcctg gcaactcgac gaacatcctc gtcaccgacg cgtatcgctg
     2941 ggccttcgag cgcggcgagc gatatggaat ggccgcggcc tacgcgacga tcatcttctt
     3001 gattcttctg ctgtggaccg tgttcggcac gcgcatcgtg cggagcaagg gagaggcgga
     3061 gacgtgagca cgaagcggcg ccctggaatc gtcgcgtcgt cggcggcgta tctcgcgctc
     3121 gtcgtcgtga cggcggtcgt gctgtatccg gtgatgctcg tctgcaagaa ggcgttcgag
     3181 cccggcaaga atttcgcgct cagtgcgtcg ccgattccgc acacgacgac gctcgacaac
     3241 tttcatcggc tgttcaccgc gcgcggcggg cacggcgagc tgctgtttct gcgccatgcg
     3301 gcgaactcgc tcatcatcgc ggtgctgacg acgatcgtcg gcgtcgtgct gtcgtgcacc
     3361 gcggcgtatg cgctgtcgcg gtttcggttt cctggccgca aggcgggact gacgacgttc
     3421 ctcgtcgtgc agatgttccc ggcgacgctg ctgctcttgc cgctctacgt catcctcgac
     3481 aagctcggcc tgctgaactc gatctcgggg ctcgtcctcg tctactcgac gaccgcgatt
     3541 ccgttctgtg tgtggacgct gaagggctac ttcgacacgc tgccgcgcga gctcgaagaa
     3601 gctgcgcgca tcgacggcgc atcaccgtgg atggtgttct ggaagatcat cctgccgctc
     3661 tcgacgcctg gcatcgcagt gaccgcgctg ttctcgttca tgacggcatg gaacgagttc
     3721 atcatggcgt cgacgttcat gacggacgag agcaagtaca cgttgcccgt gctcatccaa
     3781 tcgagcgtcg gtcagcactc cgcggactac ggcctctttg cagccggcgc ggtcgtcacg
     3841 agcttgcccg tgatgatcgc gttctacgtg ctgcagaagt atctcgtcgg cgggctcacc
     3901 gccggcgccg tgaagggcta gcgatcgcta gatgtcgatc aggccgccgc cactgctggg
     3961 cggacggtct ggacgcttcg gcgggcgaac accgccacca ccgctggagc gcttcgtcag
     4021 ctccatctcg agcgtcatgt cgccttcgga gacgtcgatc ttcttctcgc ccgagcggta
     4081 gccattcgac ttcacctcga ccttgaccgt cttcttgtcg agcgcgatct cggtcgtcat
     4141 gccgtcgacg tctttgccgt cgatcgtgac cttcgcgtcc ttcgccgcag cgtcgggctt
     4201 gatcttgatc gtcagcttca tcttcttcgc ttcgggaatc gtcggcgtgg tcggcggcgt
     4261 cgtctgcggt gtgcctgtcg ccgcggttgt cgtcgccgag cccgccccgg tagctggcgt
     4321 cgttggcggc gtcgacggtg cggctggcgt cttcaccgtc gacgatgcac cgaggccttc
     4381 aggcgcagcg gccgtcgcct tcgcgtcgtc gtccttcttc acgttgctcg ccgacggcgc
     4441 agccgcattc gcctcgctct tgccggtgcc aacaccgaac agcaggccgc agaacacacc
     4501 gactgcgaga cccgccgaga cgacgaccgg cactgccttc gatgtcccgc gcttcatcgg
     4561 gcgaggcatc ggctgcgcgc ccatcggggg accacccatg ccgggtggac cacccatacc
     4621 tggaggaccg cccattcctg gcggcatctg acccatctga ccgggataac ctggcgttgc
     4681 cataggacct cgctagaagg aaccaacggc cacgaggccg ccggcttggg gttcgaccca
     4741 cggaacgacg ccgaccttgg acggattcga cgccgggcga cgacgcgcag cttgccgctc
     4801 cgccatcgcc tgctgttgcg gacgcacctt gtagagatac cagtagctcg tgaacgctgc
     4861 cgccccgatc attccgacga ggcagatgtc ggtgatgtgg gccatcgtgc ggccacccga
     4921 ctgcgcgtcg gcgcgctcgc tgttcgacgc cttcggatcg acgtacgtcg cgtgatcgcc
     4981 gaccgcgatg atgccggtga tcgtcgcggt cagcagcagc gcgcccgtag cgccggcacc
     5041 gatgtagacc gggatcatcg gattcttctg tttcggcttg tcgggctcgg gctcggggcc
     5101 gatgtcgttg tcgacgggct tcgtcacgat cggcaccgcc tctagtgcga tcttgcgctc
     5161 gctctcggag ccggcttcga tcttgaactc gacgtctttc ggctgatagc cgacggcgga
     5221 gaacgagacg gtgatggcgc ccggcatcag cacgagcggc tccgtcatcg gcgcttcgcc
     5281 tacctgcttg ccgttgatcg agatctgggt gccgtccggc gcgaccgtca gcgtgagcac
     5341 gccgactttc atcgacatct cgtcgagctt cgcttgcgcc ttcttcacga ggtcgggctt
     5401 catgccctgc gcatcgacga cgagcttcat gtgcttgagc gcgctcggcg tatcgccggc
     5461 tttgtcttcg gcgttcgcga gctgccagtt gagcggcacc gcggcaacgt cgtcgctcgc
     5521 ttccatcgcc ttctgatacg cggtgacggc gttcgtgtac tggaccttcg cttcgtcgag
     5581 cttgttctgc ttcgcgagct tgtcgccgtt cttcatcagc aggtcgccgg cctgcagcca
     5641 cttcttcgcg accttcggat ctttcacgat cggctgatcc gcgggcggcg tgtccgcggg
     5701 cggagcatcc gcgggcggcg catccggcgg cgggtccggc ttcttcatcg acgtgtcttc
     5761 ctccatctcg atctcgcctt gtgcggacgc gacggcgaac gacgagcata ggaatgcgca
     5821 gatcagcgcc agcctcatgc tctgagctta ctacgggcgc ctgcccgctg ccccgagctt
     5881 cgcgatgtac tccaacgact tgagcggctt gccgacgagg gtctggacca tcgcgatcat
     5941 ggcgtctcgc ccggcgctgc gatctgcagg cgcgacgcga ggatccgcgt cgagcgcacg
     6001 cgccgccgag ggcgagccag ccgcggcaac cgctcgcaga tatgcaagtg tcgccggctc
     6061 gagcggccgc acacctgccg ttcggctcgt cgccgcgcaa cgcttacaaa tcgcgccgcc
     6121 gcgcgtcgga tcgaacatcg cgcccgcctc gaggtccgtc gcgccacacg ccgcgcacgc
     6181 atcgagcgcc ggcttgtgtc ccgccacgtc gagcagctcg agctcgaccg cgcgcaacgc
     6241 ggccggcgac ggcccacctt cggcaagtga ttcccacagc gcgacgatca catcgagcac
     6301 gtgcggctcc ggcgactcgg ccggcatcag cgccgcgacg agctccgcga tgtagctcgc
     6361 atgcgcgacc gcaaacacat ccgacgcaag cgtcgtccat tcgcgctcga cctccgccgc
     6421 atccagcccc cacagctcac cgcgcggacg ccggccgagc tcgtagcgac cgagcacgag
     6481 cagctgcagc gcaccggcaa accgccgctg cgatttgcgc gcaccacgcg cgaccgccga
     6541 cacgcgacca tgcgtcatcg tgtacagcgt cacgagcaga tcggattcgc gcagcggcgt
     6601 cgtcttcaag acaacgccaa gctcagcctc agccacgcgt tacgatagca gctgcgacgt
     6661 cgagtcgatc atcgtcacgc cgtcgccgct cgggctcggg cgacgcatca agtagctgag
     6721 cgtcagcgtc gcggcgatca gcagaatgat gacggcgagc gtcagcccac cctgcctcga
     6781 cgagcgatct tcgcgatcga gcagaatcgg cggcaagaac gacaaccgcg actgatcctt
     6841 ctcgctgcga tcctcgcgct cgcgatccgc gctatctgga tcggcgtcgt cgatcacgag
     6901 ggtcggcacg acgtacgcgc gggacgccgt gatgctcgga cgacgccacg gcacgctcgg
     6961 cgtgctcgcg agcggaggca tggtcggctg ccacgcgccg ctgacctgcg gctcgctcgc
     7021 gttgtcgtcg ctgactgtcg aatcggctgt cgcctcgagg ctcgagacat cgacaacctc
     7081 gatctgcgac gacgaaatgc gctggctgcc ctcggcgagg cgcggctcgt cgacggcgcc
     7141 gtcgctcgaa gtgcgcggct cgaggctcgc gttcgcagca atcgcagcat cgctcgtcgc
     7201 gtcgttcgcg ttcgtcgcgg tgttcgcgtt gttcgcgccc gtcgtcgaca cgaaaatcgc
     7261 gtcgtcggtc tttgcttcga tcgcgctcgt catgagcgac tcggcgtcga ccctcgcgac
     7321 gggcccggtg tcgagcgaga tcttcacgat ctgcgtcgtg tgggcgtcgt catcgatatc
     7381 cgtcgtcgcg aggccgatcc cgttcgcagg cggcgcgatg tcgttcgtgg atgcgttcgt
     7441 cgatgcgatc gttgatgcga gctccgcgga ggcgttcttc ttcttgcggg tgcgcggtgc
     7501 accggtcgtc ggattcttct tgcgcgagcg cttcttcttg ccttgcggct gctcgctcgg
     7561 catggactcg acgacgggcg cggcatcgag cacgaccggc gaatcgagca cgaccggcgc
     7621 atcgagcgtc atcggcgctt cgctgttcgg ctcgctcgcg gccgtcgctt cggtcgcttc
     7681 gatcatcgag ggcgcagcga cgagtggcgc cacatcgatc acgaccggcg ttacggccag
     7741 cggcgtctcg atcgcgggcg tcgcgatcgc gggcgactcg atcgcgggcg agtcggtcat
     7801 catctcgacg accgcagcga cctgcgcggc caccacgggc gtcggcgtcg cgggagtccc
     7861 ttgcgccttg cgcgcgaggc ccatcgactc gacgagagct ttcgcggtca gcgccgcagc
     7921 cggcgacttg cacgcgacgt agcgcgtgat cgcttcttgc tcgtccaggc cgacgcactt
     7981 tgcgaagctg cggacgaagc ccttgacgaa gacctcggcg ggaaggccgt cgagctcacc
     8041 gcgctcgagc ttgtcgagga tgcgtgcttg gatcttcgta atctttgcga cgtcttcgag
     8101 cgagagcttc ttctgcgcgc gacccgcgcg gagccacatc gccagattct cgcgcgctgc
     8161 ggtcattgca ccgctgtcca ccgcgtcgtc tccaccagct tgcgtgcgcg aatccatccc
     8221 tagggtccct cggcgcggca cttggttgcg atgcagctct tcagggacat cttgagacac
     8281 gcggcctgcg agaccttcgc gtcttcgacc aacccttgct gcatctgagt cttcatcagg
     8341 tagtagctgg cttcctggga cccacacgac gggtcgtcgg acaccgtctg aaacttctct
     8401 gcggctttat tccactcctc gcgagcgaag taaacccttc caagccgata agttgcgaca
     8461 cacatcttgg gttggaactg ccccacggtc agcagctcct tcgtcgcctc gacgagcttg
     8521 ttttggtgga agaacgacca ccccaaattg gctcgggtaa gagcggggtc tttgagccgc
     8581 atcgggttct cgagtgcggt ggtcaggtac gtctgcgcga ccgcgaagtc ctcgagcagg
     8641 ttgtggacga tgccgcgatc ggcccacgct tcgccgttgt cgggggcggc cttcgtcgcg
     8701 cgctcgaagt cagcgtccgc ttttgcgagg aaggtgtcga tgtccttgcc catcgcctcg
     8761 gcgtcgacgc ccgtgaggca gttgtcgacc tccatggact tcttcgcctg taacgcgcgg
     8821 atgtagctga cgagtccgcg gatgacgtag gcctcgtcgt tgccggggtt gtatgcgatc
     8881 gctttgctcg cttcgccttc agcgccttcg aggtcggcct tcgcgaggta gtcgttcgcg
     8941 atcgcgagcc gcttattgct cttttcgacg tcgtgctcga cgtggccagg gcacgccgcg
     9001 agcacagcta ggatcgcaag cgatcctgac gcgttacggc cacccacgcc cgcgaaccta
     9061 gcgcacggag gcacgcggca caacccagag atctcgcggc gctgttatag agagagcgca
     9121 tgagcgagct gcctggtccc atgacaccca cgaagacgac gaagatcgag acctcgcgcg
     9181 gcggcctcta cgcggaagtc ttcgagccga gcggcgagcc gaaaggcgtc gcgctcgtca
     9241 cgcacggata cttcgagcac tgcggtcggt atcgcgagct cgcacacgtc atcgtgaagg
     9301 cgggctggat cgcgctgacg tacgacgtgc gcggtcacgg tcagtcgccg ggtgcgcgtg
     9361 ggttcgtcga gcgcttcgat gaatacctcg acgattttcg tgatgcggtc accgcagcga
     9421 agaagctcgc gccgggcaaa ccgctcgtgc tcgtcgggca ttcgcacggc agcctcatca
     9481 cgcttcgcgc gctgacgagc gataggccgc cggacgcggc cgcggcgatc gtgtcgtcgc
     9541 cctacctcgg catgaaggtc gtggtccccg gttggaagaa gggcctcgct cgcgtcgcga
     9601 gtcgcgtggc accggcgctc acccagccaa atccgctcaa ggccgaagac ctgacgcacg
     9661 acaaggagaa gattgcggag cgtctcgccg acaagatgtg cttcgaggtc gtgcccgtgc
     9721 ggtggttcac cgaatcggcg gcggcgcagc aatatgtcga ggcgaacgcc agccgcatca
     9781 agattccgac gacgtggctc gtcggaggcg ccgacaccat cgcggacccc gcaacgagca
     9841 aacgcgtcgc agcgcgcgtt tcgggagcgc actaccacga cctcgcgggc ctcaagcacg
     9901 aggtgttcaa cgaggtagaa cgcggcaagg tgttcgccga agtgacgaaa gtcctcggcg
     9961 ccctctagcc ccttcgtcga cggcgtcacg cggcaagcgc ccgaaaccgc tcgctacccc
    10021 aacgtctaga ttggcgagat cagacggggg ctctatactc gtcagtactt cgtgggtggc
    10081 ggcgttttgg tcgtggttcc ttgatccctt gatcgtcggt tctgttcggc acggaaatgg
    10141 ataaccgttt ccgtgcggag tcccgagatg aagctcttcg cctgcatgtg caaccaacct
    10201 cagcgcctct gggccgcatt ggcacccgtg cgcgctgtgc tctccgtgcc tccgccggtg
    10261 agccggtggg gtctggcgta ttcgcaggga ggcgatgtgt tgctcgtgcg cacgccgaag
    10321 tcgagcacca agcccatcga cctgagcggt ccgctcgccg aaatcccgac cgactgcgcc
    10381 atcggccaag ccgttctcga cgacgggttc gccggcacgg acaacacgcc gccgttccgg
    10441 ttccgccgct ggatgtgggc gatggcgggc cgcccgaaca tcgacggcgc gacgccgcgc
    10501 ctgctcgagc agattccgga atacctccgt cgcaacctcc gcggtcgttc gcctggcgag
    10561 ctcgtcttcc acgtgttcct cgcgatgctg cacgacgaag gctccgtcga cgatccgaac
    10621 ctgcaggtcc acgcgacccg ccgcgcgctc gccgccacga tcaagctcgt cggtgcggag
    10681 caagccaagg ccggcgtcga gcccggtggc ctcggcaaca tcgccgtgtc gaacggtcgc
    10741 tcgatggtcg tgatgcgcct cgatcagccg ctgaagctcc gcaagctctg ggtcaacaac
    10801 gacaagaacg agcgcgacga agcgttccgc ggcgtgcttc tcgtcagcgg cggcgacggc
    10861 gatgcaaagg aagggttcga ggacgtgccc gccgatcgcg cggtgctcat cacccgagac
    10921 ctgcaagtgc agctcgcgga tctgaaccct tagctatcgc tcactgcgtt ctcacgtcgc
    10981 ggcattcaac agcgactgct cgcgagcgtg gtcctgccgt aggctcgaca gcgatgtcac
    11041 tcgattcgcg cgacaacgac gaagggtcag agccgcgccg cgcgcccggc gtgatgatcc
    11101 aacgcgtgtg cggctcgttg cagcagctgc tcgcgcgcgg cgtcgccgac tacgcgagcg
    11161 atcgggacga cgtcatcgag ctgttcctcg agcccgatga aattgctgaa ggcggcatga
    11221 tcgtgatctc gatgcgcgtc gacgtgaatt cgccaaccgg cgtgcgcgac gagctgttca
    11281 gcgcgtggct cgcggtgcgg cccaacgccg ccgagggcac gctgctcacg ccgagcgcct
    11341 ggctacccga catggttcgg cgcgtgtact tccgcgttcg cgccgcgcag cgctagtcgg
    11401 cgcggcacgt cgtacgcttc agcgcgccag gtcgtacgcg tccatgcgcg tcgccttcat
    11461 cgccttcgcg ggcggtgcct tgctgaagtt gccggatgcc gacgacgttg ctctgtcgat
    11521 cgcttcgagc gccggcgcat ccatgtactt gttgtcctgc acctcgcggc gatgctgttc
    11581 gagcacctgc tgcgcggcgg ccgcgccttg cgtctcgtac gtccgcgcgg cgtcctcgag
    11641 gacgcgagcc gtgcgtgcgg tctcgatcgc gttgaccgca ccacgatcga tcgtcgctgc
    11701 gacctggctg gcgtcatcgc tgaacgatgc gacgaggttc gtctgcgctt cgtgcacgga
    11761 gccgtcggcg acgccgcgcc attgcaggcg gaactgcgcc atctcgtacg acgagccgag
    11821 gcggatgttg ttcagcgcga catgcgagcg gatgacgacc ttgcgcgttt cgcccgcgcg
    11881 cagatcggcg acgggaatca cgatgtcttc gcggccgcgc gcgacgatcg gatagccgta
    11941 cgcctcgccg agcgtcatgc cggtgccgcg cacgacgagc cgaacatcgg cagcgacggt
    12001 ctcggtcatg ctgtcgagct ctgtccggaa catctgcgcg aggttcgcgg tgtcctcgac
    12061 gaagtagtag tggccgtgac cgacgtctgc gaggcgcgtc atcgtgactt catcgaagtc
    12121 gaggccgacg ccgacagtcg agatcgagac gcccttcgcg gccgtctctg tggcgagctg
    12181 cgcgagctcg tcgcgatcgt agaggccgct gttcgcctgg ccgtcggaga tgagcaccat
    12241 gcgtgcgatg ccttggcccg agtacggacg atcgccgccg agcggagagc gcgcgagctg
    12301 cgccgcgcct tgctcgatgc cacatgagat gcaggtgttg ccgtcggacg taatgctgtc
    12361 gatcgctcgc gccgcggcga gcttgttcgc atcgttcgcc aggctcatct ggaacacggt
    12421 ctcgacgctc gtcgaatacg cgatcaccgt gaacgcatca ccgggcgaga gcgcatcgac
    12481 gagctttgca gcagctgcct ttgcattcgc gagcgcctcg ccttccatcg aacccgaccg
    12541 atcgatcacg atcgcgagtg agagcggcgg acgaacctgc gcgcggcctt tcggcatcgt
    12601 gatcgtgacg gcgatgtctt gatcgcgatc ggtcgcgagc agcgtcttcg acgcgaactt
    12661 cgcgctgagg atgtcgctcg acaccgcatc gggcatcgcg ggctcggggg accgcggggt
    12721 caggagccag gcagcgatca gggaggcgcc ggcggcgccc agaacgagcg aagtcctggt
    12781 ccgatgagca gtgagcatgt ggcgtatcga cgcatgactt ctgctgacga tctgggctgt
    12841 gaacggggtc agaaacgaaa ttcgactcag atttagccta catgccttgg aggctactcg
    12901 agtagccacc gcatcagtgc caactcgacg aatctactag ccattagtag taatgaagat
    12961 gctgcttgac cgattgcgtc aaagttacta aagc
//
LOCUS       DATLBZ010000174         5010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_168562, whole genome shotgun sequence.
ACCESSION   DATLBZ010000174 DATLBZ010000000
VERSION     DATLBZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168562"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1067)
                     /locus_tag="VMZ53_09170"
     CDS             complement(141..1067)
                     /locus_tag="VMZ53_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28669.1"
                     /translation="MRSENVESLEALFAAAPRKSIVRDDDEDEEDEDDDDDDEEDEDE
                     EADDDDDDDDDEPDDDAEAATDEVADDWVGGGAVAPIADDDEEVVEKEEEEEDDDEDD
                     ADVGGWRGGDKPPWGGDEDEDEDEDEDEDEDDDEVAAVGEDDDDDEEEEEDDDDDAPA
                     PTPVRKPPVRIVRDDDDEEESSSSDDDDDDRDPIDPEDRPPRGPLPPRPPKPLPSGKP
                     EPIRVPGKPIAGKPAPVPAKPPAAPAKAPVPAAPAKAPPPAKAPAKPAAKAPAAKAPA
                     AKPAAKAPAKPAAKPAAAAKKPAAKPPAKKKK"
     gene            1154..2560
                     /locus_tag="VMZ53_09175"
     CDS             1154..2560
                     /locus_tag="VMZ53_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28670.1"
                     /translation="MRRPLHFVIASVLLTVAPAAEADSVDFYGTANGSVATTDNVSGL
                     EAGKPGKRGDVFSDIRPGFLVTYYAPRMIHEINTEVDFLYHIFTAKPTVTFRAGWKAF
                     FITSPRSEATINADASKGQLNALSASITPDTSIPTQVLPPGRVDVESVTGSQNLSWVA
                     TEGSRIWQRAFGRYGTTLDEVANMDDVTTKSWETGIAFGVDRTYRHNNFLLELGGSYV
                     YMEKLDPSGRQMGSRLDRQWNPRAVVVWQHDITKQWSSNIDAGGVYVMPTGVDPYNPG
                     QERHNGFFPVFGGVAAYTDVWGRAQVAARRQVTPNLFIAQNTLSDSLTLTAAMPLTFF
                     DKDAQKRAPKVVGIGNAGVERTRLIDPVTGGLSSTYHVARIDFGVGWSPRPGQTFGLR
                     YELSYQNGTTTAAEMQVPEFVRNTFYFTFSLRYPEDILVKVPRRGQSVRADKKDLAPI
                     GAEPVIVDPQEFLEGGGR"
     gene            complement(2557..3765)
                     /locus_tag="VMZ53_09180"
     CDS             complement(2557..3765)
                     /locus_tag="VMZ53_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28671.1"
                     /translation="MRDAVREAIDRCVRPTLVFDLARIVDNMRVVAEAARAARISALF
                     AMKSFPHRRVVEVARRTLAGFDAASVAELRLALEAAAADARSNSAAHELISIADPTGA
                     AIAYAADAFAEPTRAAIGYAADANENRRVIIACETVEQVRAAPAHADIAIRVSASITE
                     RDPAVGAILDGTGHRRSRFGLERAEEIRVLRDAALRSDARRRVGLHVHHGPVTATSAE
                     RFIATARAAIELARSAGVEPDFIDVGGAWHGIGIANIAAAFAAIRAGIDDAMRARGDE
                     ANGDDAIEILVEPGRLLVEDAGFACGPTTSTRDLGDGRLLVTTALSRICHLRWSQVEL
                     VAKAPHPGTGVKAIIVGPTCYEEDVVGEWTVDPSQIEKRVVVRNVSGYAVAWNKSFGG
                     VPEADVVFVD"
     gene            complement(3765..4163)
                     /locus_tag="VMZ53_09185"
     CDS             complement(3765..4163)
                     /locus_tag="VMZ53_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28672.1"
                     /translation="MRARSFVRMDGVLREAGRLVAIGGIAGLAGCSDGPSARIIVPLP
                     DTTVYASVELRMEGHKLERTTQTKIYVDLTQYTGELIDNSLPSDCDDCNFVIGFAGPT
                     ITNGAHTISAHFYDGEVEIASDSVPLVFAR"
     gene            complement(4510..>5010)
                     /locus_tag="VMZ53_09190"
     CDS             complement(4510..>5010)
                     /locus_tag="VMZ53_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HUS28673.1"
                     /translation="ELERATCDCIDIGSVDDGDEPARAKRSIPKAVKRHVLHRDGYKC
                     RVPGCRVTENIDCHHIVYFMHGGEAVPMNLIALCEGHHLAVHAGSLVIAGDAVKTTFT
                     WSKQSKWKIETRVVECTAALRSRGVDKQQIKAVVAATRAHVGNQDLTTRQWMEIALTK
                     CVPTSS"
BASE COUNT          774 a         1815 c         1498 g          923 t
ORIGIN      
        1 actcggccgt gcgccaccac atgccgcaga tcgacatcaa gatccgcgtg cccgacttct
       61 tcgcgatcag cgtgctcgtg ttcggctacg ccttcgtcgc gctgcccctg tacctgaagt
      121 acaagggctt cctctagccg ctacttcttc ttcttcgcgg gcggcttcgc agcgggcttc
      181 ttcgcagccg cagcaggctt cgcagcaggc tttgccggcg ccttcgcagc gggctttgcc
      241 gccggagcct ttgcagccgg agccttcgcc gctggcttcg caggcgcctt cgccggcggt
      301 ggtgccttcg ccggtgctgc cggaacaggt gccttcgccg gagcggccgg aggcttcgcg
      361 ggaacaggcg cgggctttcc ggcgatcggt ttccccggaa cgcggatcgg ctcgggcttg
      421 cccgatggca gcggcttggg cggacgcgga ggcagcggcc cgcgcggggg gcgatcctcc
      481 ggatcgatcg ggtcgcgatc gtcgtcatca tcgtcggagg aagaggattc ctcctcgtcg
      541 tcgtcgtcgc gcacgatcct caccggaggc ttgcgcacgg gcgtaggcgc cggcgcgtcg
      601 tcgtcgtcgt cttcttcttc ttcctcgtcg tcgtcgtcat cctcgccaac cgccgcgact
      661 tcgtcgtcgt cctcgtcttc gtcctcgtcc tcgtcttcgt cttcatcctc gtcgccgccc
      721 cacggaggct tgtcgccacc gcgccagccg cccacgtcgg catcatcctc gtcgtcgtct
      781 tcttcttctt cctctttttc gacgacttcc tcgtcgtcgt cggcgatcgg agccactgcc
      841 ccaccgccga cccaatcgtc ggctacttcg tcggtggcag cttcggcgtc gtcgtcgggc
      901 tcgtcatcat catcgtcgtc gtcgtcgtca gcctcttcgt cttcgtcctc ttcatcatcg
      961 tcatcgtcgt cttcgtcctc ttcgtcttcg tcgtcatcgc gcacgatgct cttgcgaggt
     1021 gctgcggcaa agagggcttc gaggctctcg acgttctcac ttcgcatacc ggggcattct
     1081 aaataacttg agcgtgagtt ctgtcaaccc gggcagcctc tttcgttaga acgctgcatg
     1141 ctacaaacca cccgtgcggc gcccccttca tttcgtgatt gcgagcgtgc tgctgaccgt
     1201 cgcacccgcc gccgaggccg atagcgtcga tttctacggg accgccaacg ggagcgtcgc
     1261 gaccacggac aacgtgagcg gcctcgaagc gggcaagccc ggcaagcgtg gcgacgtctt
     1321 cagtgacatc cgccccggct tcctggtcac ctactacgcg ccccggatga tccacgagat
     1381 caacaccgag gtggacttcc tctatcacat cttcacggcc aagccgaccg tgacgttccg
     1441 tgcgggctgg aaggcgttct tcatcacgag cccacgctcc gaagcgacga tcaacgccga
     1501 cgcgagcaaa ggccagctca acgcgctcag cgcatcgatc acacccgaca ccagcatccc
     1561 gacgcaggtc cttccgcccg gccgcgtcga cgtcgagagc gtcactggga gccagaacct
     1621 ctcctgggtc gcgaccgaag gctcgcgcat ctggcagcgc gcgtttggcc gctacggcac
     1681 gacgctcgac gaagtcgcga acatggacga cgtcaccacg aagtcgtggg agaccggcat
     1741 cgcgttcggc gtcgaccgca cgtaccgcca caacaacttc ctgctcgagc tcggcggctc
     1801 ctacgtctac atggagaagc tcgatccaag cggtcgccaa atgggcagcc gcctcgatcg
     1861 ccaatggaac ccgcgcgcgg tcgtcgtctg gcagcacgac atcacgaagc agtggagctc
     1921 caacatcgac gcaggcggcg tctacgtgat gcccaccggt gtcgacccat acaacccagg
     1981 ccaagagcgc cacaacggct tcttcccggt cttcggtggc gtcgccgcat acaccgacgt
     2041 gtggggccgc gcgcaagtcg ccgctcgccg ccaagtgacg ccaaatctat ttatcgcgca
     2101 gaacacgctg agcgacagcc tcacgctcac ggccgcgatg ccgctcacgt tcttcgacaa
     2161 agacgcccaa aagcgcgcgc ccaaagtcgt gggcatcggc aacgcaggcg tcgagcgcac
     2221 gcgcttgatc gatccggtca cgggcggcct ctctagcacc taccacgtcg cgcgcatcga
     2281 cttcggcgtt ggctggtcgc cgcgcccggg ccagacattc ggcctgcgct acgagctctc
     2341 gtaccaaaac ggcaccacga ccgccgccga gatgcaggtg cccgagttcg tgcgcaacac
     2401 gttctacttc acgttctcgc tgcgctaccc ggaagacatc ctcgtcaagg tcccgcgccg
     2461 cggccaatcc gtccgcgccg acaaaaaaga tctcgcgccg atcggtgccg agcccgtcat
     2521 cgtcgatccg caagagttcc tcgagggcgg cggccgctaa tcgacgaaca cgacgtcggc
     2581 ctcgggcacg ccgccaaagc tcttgttcca cgcgacggca tacccgctga cattgcgcac
     2641 gaccacacgc ttctcgatct gcgacggatc gacggtccac tcaccgacga catcctcttc
     2701 gtagcaagtc ggcccgacga tgattgcctt cacgccggtt cccggatgcg gcgccttcgc
     2761 gacgagctcc acctggctcc agcgcaaatg gcagatgcgc gagagtgccg tcgtgacgag
     2821 cagccggcca tcaccgaggt cgcgcgtgct cgtcgtcggt ccacacgcga agcccgcgtc
     2881 ctcgacgagg aggcggccag gctcgacgag aatctcgatg gcgtcgtcgc cgttggcttc
     2941 gtcgccccgg gctcgcatcg cgtcgtcgat gccggcgcga atcgcggcga aggccgcagc
     3001 gatattcgcg atgccgatgc cgtgccacgc gccgccgacg tcgatgaagt cgggctcgac
     3061 gcccgcgctg cgtgctaact cgatcgctgc gcgcgcagtc gctataaagc gctccgcgga
     3121 tgtcgctgtc accggtccgt gatgcacgtg caagccgacc cgccgccggg catccgaccg
     3181 cagcgcagcg tcgcgcaaca cgcggatctc ttcggcgcgt tcgagcccga atcgcgatcg
     3241 acgatgtcca gttccgtcga ggatcgcgcc gaccgccgga tcgcgctccg tgatcgacgc
     3301 cgacacgcga atcgcgatgt ccgcgtgcgc gggcgctgcg cgaacctgct cgacggtctc
     3361 gcacgcgatg atcacgcggc gattttcgtt tgcgtccgct gcgtacccga tagcggcacg
     3421 ggtcggttcg gcgaatgcgt ccgctgcgta tgcgatagcc gcgcccgtcg ggtccgcgat
     3481 cgagatgagc tcgtgagccg cgctgttcga tctcgcatcg gccgccgccg cttcgagtgc
     3541 gagtcgtagc tcggcgaccg atgccgcgtc gaaaccggcg agggttcgcc gtgcgacctc
     3601 gacaacgcgt cgatgcggaa acgacttcat cgcgaacagc gccgatatac gcgccgcgcg
     3661 cgcggcctcg gccaccacgc gcatgttgtc gacgattctc gcaaggtcga acacgagcgt
     3721 cgggcgcaca catcgatcga tcgcctcgcg cacggcgtca cgcatcaacg tgcgaacacg
     3781 agtggcaccg aatccgacgc gatctctact tcgccgtcgt agaaatgcgc ggagatggta
     3841 tgcgcgccgt tcgtgatcgt cggtccagca aagccgatca cgaagttgca gtcgtcgcag
     3901 tccgacggaa gcgagttgtc gatgagctcg ccggtatact gcgtgaggtc gacgtagatc
     3961 ttcgtctgcg tcgtgcgctc gagcttgtgc ccctccatgc gcagctcgac gctcgcgtac
     4021 acggtcgtgt ccggcagcgg cacgatgatg cgagcgctcg gcccgtcgct gcatcccgcg
     4081 agccccgcaa ttcccccgat cgcaacgagg cgccccgctt cacgaaggac accgtccatc
     4141 cgcacgaagc tgcgagctcg catgcccacc agtctacacc cgggggcacc gtcacctcac
     4201 cggtggtgcc gtcacctcac cggtggcgcc ggctcctcac ccgtcgcatc gtttcctcac
     4261 cggtcgtctc ctcaccggtc gtgccgtctc ctcaccgggc caccgtctcc tcaccggtcg
     4321 tgccgtctcc tcaccgggcc accgtcacct caccggtggt gccgtcacct caccggcggc
     4381 gccggctgct cacccgtggt gccgtcacct cggtggcgcc ggctcctcac cggtggtgcc
     4441 gtcacctcac cggtggcgcc ggcacctcac cggtggcgcc ggctcctcac cggtcgtgga
     4501 tccagcactt caactcgagg tcggcacgca tttcgtcagc gcgatctcca tccactgtcg
     4561 cgtcgtcaga tcctgattac ccacgtgggc acgcgtcgcc gcgacgacgg ccttgatctg
     4621 ctgcttgtcg acgccgcgcg agcgcagtgc cgccgtgcac tcgacgacgc gcgtctcgat
     4681 cttccactta ctctgcttgc tccacgtgaa tgtcgtcttc acggcatcgc ccgcgatgac
     4741 gagcgagccc gcgtgcaccg cgaggtgatg tccttcgcag agcgcgatca agttcatcgg
     4801 caccgcttcg ccgccatgca tgaagtagac gatgtggtgg caatcgatgt tctcggtcac
     4861 tcgacaccca ggcacgcggc acttgtagcc gtcgcgatgc agcacgtggc gcttcaccgc
     4921 cttcggaatg ctgcgcttcg cgcgagctgg ctcgtcgccg tcgtcgacgc tgccgatgtc
     4981 gatgcagtcg cacgtcgcgc gctctagctc
//
LOCUS       DATLBZ010000175         3440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2403730, whole genome shotgun sequence.
ACCESSION   DATLBZ010000175 DATLBZ010000000
VERSION     DATLBZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403730"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="VMZ53_09195"
     CDS             complement(<1..1608)
                     /locus_tag="VMZ53_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HUS28674.1"
                     /translation="MRRWLLIALVGCSHPAATPPSTVPAPTVQRLRAPPALDPDVRGS
                     AYLATVALQLQPGWGQFLDDCRLRLPATHALNEFSLAATADLAIDPRGQVVDVRIASS
                     GNADFDRAVRDALRDISPLPSPPRELWSDDDRVHLTWLFARDRRQAGPATAAVVDVEL
                     PLAGVVSRLVRERDLSRAARRILRAPSGDDRDAATRELAIAALREAVDASDTTVRRAA
                     VEAIRRAGVSELAPDVRALLVATGDSELRALATTTLAALHDEQSAAAILELLARDIVE
                     NESLARTDVNALLALNHERDVVALLRAALANQGRAEPNAIALAVAGLVAMPEVSAQIG
                     TWLASKHARTRAAACEALGSFAPADAKKWLVKGMSDRDASVRAACTRSVRDPASVRAK
                     LRALAKDRDIATRAAAVAHLARLPDEVVSVDDDPASQVRAAYAAAVTPTHDLSALLAD
                     RDPDVRAAAWQTHARGGAPTLKAEAADAADDPSPFVRRALIAALDDDETISRLARADV
                     DGDVRRDALVRWAERRGRASVADSLLTN"
     gene            1648..2475
                     /locus_tag="VMZ53_09200"
     CDS             1648..2475
                     /locus_tag="VMZ53_09200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS28675.1"
                     /translation="MRSYVVCLSLLLGACRGGESKKPPVAIPMVPGDEKAPADVQQER
                     RDFISDVTAFTKLPGDQVVAKLVDNTGMKKEWEAWEGSAAWTDERIKQFYKQTQNYIF
                     DLGGWHLWDLAKRESDRAMPDELRKAMPEELKKPGAKPRVLDFGGGVGFNAMILAQAG
                     FDVTLADLDSVTLSFAKFRAERHGVTMKYWKSDVEEMPPEAKYDVILCMDVMEHLPAD
                     ELKKTVDKLVKLKTKTTQIVIHAPFGRTAQHPMHLDASEDTLKQIARLEHELPPDAQ"
     gene            complement(2476..3270)
                     /locus_tag="VMZ53_09205"
     CDS             complement(2476..3270)
                     /locus_tag="VMZ53_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HUS28676.1"
                     /translation="MRRLLVCLPVVAGCASAASSGVDEPPQYLALGDSVAFGYDPRTD
                     HSTDSGYAELLAANQQIALTNASCPGEASGGFIAPDGNDNHCRENKQAYSLHVKYTGT
                     QLAFAIDFLRDHPNTELVTIDLGGNDVGKLNATCAGALPCILGGIVTTLEEYDRNMQY
                     IFGELRKVYDGPLLGIGIYNPYPGDSTAEWGLGKLNTLLAAQLRTYDGTFVDGLAAFA
                     AVSADPCADGLLIKMPDGTCDIHPSPKGHEILAAAVQNVLTNGAAQ"
BASE COUNT          589 a         1107 c         1196 g          548 t
ORIGIN      
        1 gttggtgagg agcgagtcgg caacggacgc acgcccgcgg cgctcggccc agcgtacgag
       61 tgcatctctg cgaacatcac cgtcgacgtc ggcacgcgcg agccggctga tcgtctcgtc
      121 atcatcgagc gcggcaatca gcgcgcgacg cacgaacggt gacggatcgt ctgcggcatc
      181 ggccgcttcg gccttcagcg ttggcgcacc accgcgcgcg tgcgtctgcc aggcggcggc
      241 gcggacgtcc ggatcgcgat cggcgaggag cgcgctcagg tcgtgcgtcg gcgtcacggc
      301 tgctgcgtag gcggctcgca cctgcgatgc aggatcatcg tcgacgctga cgacctcgtc
      361 gggcaacctg gcgaggtgcg cgaccgcggc cgctcgtgtt gcaatgtcgc gatccttggc
      421 gagggcgcgc agcttcgcgc gaacgcttgc gggatcgcga acgctcctcg tgcacgccgc
      481 gcgtacgctc gcatcgcgat cggacatgcc cttcacgagc cacttcttcg catctgccgg
      541 cgcgaagctg ccgagcgcct cgcatgcggc cgctctcgtg cgtgcgtgct tgctcgcgag
      601 ccacgtcccg atctgtgcac tgacttcggg catcgcgacg aggcctgcga ccgccagcgc
      661 gatcgcattg ggctctgccc tgccctgatt ggcgagcgcg gcgcggagca gcgcaacgac
      721 gtcccgttca tggttgagcg cgaggagcgc gttcacgtcg gtgcgtgcga gcgactcgtt
      781 ctcgacgata tcgcgtgcaa gaagctcgag aatggcggcg gcggactgct cgtcgtgcaa
      841 cgcggcgagc gtcgtggtcg ccagcgcgcg cagctcggaa tcgccggtgg ccacgagcag
      901 cgccctcaca tcgggtgcga gctcgctgac gcctgccctg cgaattgcct cgacggcggc
      961 gcgccggacc gtcgtatccg atgcgtcgac cgcttcgcgt aacgctgcga tggctagctc
     1021 tcgtgtcgcg gcgtcgcggt catcgccgct cggcgcgcgc aggatgcgcc gtgcagcacg
     1081 cgacagatcg cgctcgcgga cgagccggct gacgacaccc gcgagcggca gctcgacatc
     1141 gacgacagcc gcggtcgccg ggcctgcctg cctgcggtcg cgcgcgaaca gccacgtcaa
     1201 atgcacgcga tcgtcgtccg accacagctc gcgcggtggc gacggcagcg ggctgatatc
     1261 gcgcagagcg tcgcgaacgg cgcgatcgaa gtccgcattc cccgagcttg cgatgcgcac
     1321 gtcgacgact tggccgcgtg gatcaatcgc gaggtccgcc gtggccgcga gtgagaactc
     1381 gttgagcgca tgggtggcgg gcagcctcag ccggcaatcg tcgaggaact ggccccaccc
     1441 gggctggagc tggagcgcga ccgtcgcaag atacgccgag ccacgcacgt cgggatcgag
     1501 cgcaggcggt gcacgcaaac gctgaaccgt cggcgccgga accgtgctcg gcggcgtcgc
     1561 agccggatgg ctacaaccga cgagcgcgat caggagccag cggcgcaccc acgtacgata
     1621 gcgcagcagt gataggttcg ccgtcctatg cgtagttacg tcgtttgcct gagcctcttg
     1681 ttgggcgcgt gtcgcggcgg tgagtcaaag aagccgccag tcgcaatccc gatggtacct
     1741 ggcgacgaga aggctcccgc agatgtccag caggagcggc gcgacttcat ctccgacgtc
     1801 acggcgttca cgaagctgcc cggcgatcag gtcgtcgcaa agctcgtcga taacaccggg
     1861 atgaagaagg agtgggaagc gtgggaaggc tcggccgcgt ggaccgacga gcgcatcaag
     1921 cagttctaca aacagacgca gaactacatc tttgatctcg gcggctggca cctctgggat
     1981 ctcgccaagc gcgagagcga tcgcgcgatg ccggacgagc tgcgcaaggc gatgcccgag
     2041 gagctgaaga agccgggcgc gaagccgcgc gtcctcgatt tcggcggcgg tgtcggcttc
     2101 aacgcgatga tcctcgcgca ggcaggcttt gacgtgacgc tcgccgatct cgattcggtg
     2161 acgctgtcgt ttgcgaagtt tcgcgcggag cgacacggcg tgacgatgaa atattggaag
     2221 tccgacgtcg aagagatgcc ccccgaggcg aagtacgacg tgattctctg catggacgtc
     2281 atggagcact tgcccgccga cgagctgaag aagaccgtcg acaagctcgt gaagctgaag
     2341 accaagacga cgcagatcgt gatccacgcg ccgttcggcc gtaccgcgca gcatccgatg
     2401 catctcgacg cgagcgagga cacgctgaag cagatcgcgc ggctcgagca cgagctgccg
     2461 ccggacgcgc aatagctact gcgctgcgcc gttcgtcagt acgttctgca cggccgcagc
     2521 gagaatctcg tgacccttcg ggctcggatg gatgtcgcac gtgccgtcgg gcatcttgat
     2581 caagaggccg tcggcgcatg gatccgcgga gaccgcagcg aacgcggcga gaccgtcgac
     2641 gaaggtgccg tcgtacgtgc ggagctgcgc cgcgaggagc gtgttcagct tgccgaggcc
     2701 ccactctgcg gtggagtcgc cgggatacgg attgtagatg ccgatgccga gcagcggtcc
     2761 gtcgtagacc ttgcgcagct cgccgaagat gtactgcatg ttgcggtcgt actcttcgag
     2821 cgtcgtgacg atgccgccga ggatgcaggg cagcgcgccc gcgcatgtcg cgttgagctt
     2881 gccgacatcg ttgccgccga gatcgatcgt gacgagctcg gtgttcggat ggtcgcgaag
     2941 aaagtcgatc gcgaacgcga gctgcgtgcc cgtgtacttc acgtggagtg agtaggcttg
     3001 cttgttctcg cgacagtggt tgtcgttgcc gtcgggcgcg atgaagccac cgctcgcttc
     3061 gcccgggcag gatgcgttcg tcagcgcgat ttgctggttc gcggcgagga gctcggcgta
     3121 accggagtcg gtcgagtgat cggtgcgcgg gtcgtagcca aacgcgaccg aatcgccgag
     3181 ggccaagtac tgcggcggct cgtcgacgcc ggagcttgcc gccgacgcac aacccgcaac
     3241 caccggtaga cacaccaaca agcgccgcat ttggacacgg gtctaagcac gggtcgtgcc
     3301 gcctgccccg cggctcaacg cagcggaacc gcagcgcgtc aacgcgcccg acgcatggcc
     3361 agccgagttg ccggcaagac cgattacatc gtcgggacgt cgaacgtgag cgtcgggttc
     3421 gagatacccg cgccgacgaa
//
LOCUS       DATLBZ010000176        17314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3067588, whole genome shotgun sequence.
ACCESSION   DATLBZ010000176 DATLBZ010000000
VERSION     DATLBZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17314
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3067588"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VMZ53_09210"
     CDS             <1..613
                     /locus_tag="VMZ53_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUS28677.1"
                     /translation="GEDGAQGKDFIKTDASITHGNSGGPVIDDNGRLVGIAAAFRTRM
                     TATGGVIETAQVGLVRPLQAASNLLAYAAAGWTPREGYTDVELTPTAIEAAAEGVRIY
                     TTVVDDANDSPVRDATVMVLKAGVNTTTIDMNRVDDQAIAWGKTNALGEVRLKQLVPI
                     PGTYTVMVIAPGYEPLIGESELHLDDKTPPSFDPWGKIGLRSR"
     gene            complement(615..2048)
                     /locus_tag="VMZ53_09215"
     CDS             complement(615..2048)
                     /locus_tag="VMZ53_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS28678.1"
                     /translation="MPLSSLALDFDQLPVPAWVFELDGTIARLNEAAALLHGHAAADI
                     VGKKAWEFAPGGEHVWPELVRNARARGQYRADITLATPHGPRSIHYLAVLRTEGERTF
                     FLVLGVERDPSVEAARAAEVHAKQRVEALGLVAGGIAHDFNNQLTSVLVEASAARESG
                     QLSEATAESLTRIEAAANRMAQLTRQLLAYAGRGRFVSELLDPDQLVRDARDQLAQLA
                     KPVDLAITTGAGRVVIEADRALLVQVLANLVANAAEAQSQAPIEITTSAASGRWQIEI
                     RDRGTGMDPDTVARIWDPFFTTKRGHDGLGLSAVVGIVRRLSGEITVESRPANGSLFT
                     LRFPLAAGEVKPRRAPKQTAHGISLAGMRALIADDEPAVRTTIRRLLERRNATVVTAA
                     SGTEAEARLREGTYAFIVLDVMMPGLTGYQLLPIARETNPDARVLLMSGYTEVERSVG
                     GDDGPDAFLEKPFTSKSFSDAIDELLS"
     gene            2176..3684
                     /gene="pcnB"
                     /locus_tag="VMZ53_09220"
     CDS             2176..3684
                     /gene="pcnB"
                     /locus_tag="VMZ53_09220"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HUS28679.1"
                     /translation="MPSLDRTAIDADADRVVRKLTRAGYKAYLVGGCVRDLLVGRTPK
                     DFDVATSATPNEIKATFRNSRIIGRRFRLAHVFFGSKIIETATFRANPRDEDDHDLLI
                     RRDNVFGTEAEDAKRRDFTINGLFYDVEREEVIDHVGGLADLDARLVRTIGDPDIRFQ
                     EDPVRMLRAIKFAARLSFGFEPATWRALLRWRSEISKCAPPRLLEEIHRLLRGGAARR
                     SFELMVETGVLAVLSPYLAGLLEGTGAPTTAPPIADTAPRPTVPRVEYDDEDPAEHDP
                     DDPDEEEGSLPVNGEPEAAPAEETEELDDDEREWHRVWADEPPARPAHAMAIKLSFLS
                     APEMAKRRALAWEALGQLDAAVADGRDPSNAVAFGLLLAAFLPEELRGGELHGALNEL
                     AHPLIQQLHVTRRDSERLKYILIAQRKLAAAKKRGVQAELAGGRELIDDALLLYALLE
                     RAGGNESAIPPAAIATGEGRDDEEDSDDPNRPRKRRRRRRGGRRRGGNAANA"
     gene            3684..4574
                     /locus_tag="VMZ53_09225"
     CDS             3684..4574
                     /locus_tag="VMZ53_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS28680.1"
                     /translation="MPRLPAGLIEDLVDAGSREHYADAALYDYEYRRRRADVAFYREL
                     AKQRLGDPAKLKPGSARILELGAGSGRITVQLARAGYEVVAVDASAAMLSKLRDRVAA
                     LPQAVAKRITVVQGDLRDFAIDGKKFPLAIAVFNVIEHLYTRVEVDACLRRVVEHLAP
                     NGAFAFDVQLPDLAWLIRDPEKRWARTRFTDPTTGRAMLYSTNHDYDPVGQIVLIRLY
                     YEPVDGKGPTKIVKLSQRKFFPAELEALVAHAGLRMVERYGDFGWQALHPAAESQVLV
                     CERAISRKSSSRNSRFSSRI"
     gene            4765..5112
                     /locus_tag="VMZ53_09230"
     CDS             4765..5112
                     /locus_tag="VMZ53_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28681.1"
                     /translation="MGEKIAKLGIKRDNDMMYYIKGGDVWATPRKKPGQPKGKAAKVA
                     AAGIDMDYSKFLYYLDGDGDVARKERQVGGSKRKSSGKKSAAKKPAKKTAAKKTAKKG
                     AAKKSGGKKGKRK"
     gene            5311..6420
                     /locus_tag="VMZ53_09235"
     CDS             5311..6420
                     /locus_tag="VMZ53_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28682.1"
                     /translation="MVMLRLLSRGLLVGALVLGYTAHHERLAYADDDEEEEEDDGGGD
                     KGGDGEGDDEEGEGEGDDDKDQPPVTAGGLFTMTSYPVREISRPLTLTQGITQLRASA
                     GTDLSAKGAFESGGLSLEAVYGQADNFNIIGGLTNAYNFKQFGFYAGFDAALAYDIVD
                     FRLAANVHRNALPVYADFCNPPASPNEMPNGMGMCSDPATQQVQALPSGDYLAGSTQF
                     SVDIGFPFRYAIKPEVAIIALYTLMQIDFNSAKTDHVTLSVEDDPNNPGKKKVRSANT
                     GDGYKPDLNPSVGIATNPLPPLSVVIFAQLRIPEFDTSAGAFQVPVTGRIEFSPNQKF
                     DLGLEFTLLNVKPPEGQSPIDNRFLALFIQSRFGK"
     gene            6417..7670
                     /locus_tag="VMZ53_09240"
     CDS             6417..7670
                     /locus_tag="VMZ53_09240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28683.1"
                     /translation="MKVCPRCFTRFPDGERFCLHDSAVLVEEEDIARLGHNIGNYRLD
                     KILGRGGMGTVYFGEHVYIKKPVAVKVLHPQFARYQEAIHRFLREARAATSINHPNIV
                     DVTDFGILPEGPVYFVMEFLEGKSLEDLIEKDGAVELHRALNIANQIALALDAAHAVG
                     VIHRDLKPDNIMLLKKPGRRDIVRMTPDQQWITEREESYDFVKVLDFGIAKTLIQDEL
                     MAETVQGAVFGTPEYMSPEAARGEDVDHRADVYSLGVILFDMLCGRPPFEAPQSSEVL
                     AMHIHQPPPSPREFAPHREITEQAEGVILKAMQKDPKQRYQDMVEFRRDLEISYGSIS
                     YRRRGAPVRGLEPRGLEARAPKKRLTEELDEWITNDPSGMSVEQARMVSLVETAEKAF
                     TPSGMSPAEAERIANALDRALDDDE"
     gene            7727..7969
                     /locus_tag="VMZ53_09245"
     CDS             7727..7969
                     /locus_tag="VMZ53_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28684.1"
                     /translation="MSNQASQSGQITLRKDVKFNGVKVFSATMVADRDQLGEKVTAWI
                     AANKEHKKVTDIVITQSSDEAFHCIAITVFYWEERS"
     gene            7966..9036
                     /gene="serC"
                     /locus_tag="VMZ53_09250"
     CDS             7966..9036
                     /gene="serC"
                     /locus_tag="VMZ53_09250"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HUS28685.1"
                     /translation="MTTRKLNFSSGPAVLPEEVLEKAQAAILDLDGSGIGILEHSHRG
                     KEFGRVLDRAEAAVRQVGSIGDDYAVLFVTGGATQHFAMIPQNFLGAGETADYCHTGI
                     WSGKAIDEAKRVGMVHVACDGKPDNFASIPSPLNWSKQPRYVHYTSNETIYGTQWPAP
                     PDAPAPLVCDASSDIFSRPLSLANHALIYVGAQKNLGPSGISLVIARKDFVETGERHL
                     SPLAQYRTYVAERSMHNTPNTFGIWMIAETCEWILRQGGLVAMAERNLRKAKLLYDAL
                     DASKVWRPHAKAGSRSLMNVTFRGATPELEDAFIKRAEERGMSGLRGHRSVGGMRASI
                     YNAFPEDGVRKLVELLHEMEPS"
     gene            9051..9701
                     /locus_tag="VMZ53_09255"
     CDS             9051..9701
                     /locus_tag="VMZ53_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28686.1"
                     /translation="MTFLTDEGEKELEAAVAAIEAVSSAEIVVAIRPRVRGSIVQHVI
                     VALVMSIGMLAFTLYSPIEFRLWHILTLPVMAGLAGGLLVEAVPALYRFLLPAQLRSV
                     HVYDAACAAFVQRGIHATRDRTGILVYVALREQCVELVGDLGVREHVGAETLIAWSGI
                     LEGALPNGAKAVAKELAALAPELAKALPRRADDIDELANGVQLLSARPRQQGKAAR"
     gene            9698..10780
                     /locus_tag="VMZ53_09260"
     CDS             9698..10780
                     /locus_tag="VMZ53_09260"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HUS28687.1"
                     /translation="MTMPKRVVLLLLLATATAYARPGSSGTSTTYHPSSTYHYNGSHT
                     RGSGDLGAGGVIAIFLGVLIFVGLWSAIAHARQGSRTVQVTTPPTPRLDFTPLRTANA
                     SFSRFSFEDTVAELVRAELARRHEAKPEAVLVADIRPSAATSTHVTCTVEATIGGTYN
                     VGHWTFERTADNTWRVDVYRDISLGRGPLLISTGREVGNDLPTLTDEQAIVRFTQIRD
                     EDKTTSWDAFTARVRETYAKLNDAWNKQELADVDGYVTPRMIEWLRFWYAEYRRQGLV
                     NHHADAEVRHIGLAQVTREPNLDAVTVRVYAAGFDFTTKANTTDKVAGSQTEKREYTE
                     YWTFVRAKGSPSWLLTKIEQDELYLG"
     gene            10783..11979
                     /locus_tag="VMZ53_09265"
     CDS             10783..11979
                     /locus_tag="VMZ53_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUS28688.1"
                     /translation="MRPPPSIARFVSFPVVAMISLAAMFVTGAVMFGKTEIEPFIMGS
                     IAFETQPWRLASSALPHAGPIHLLFNVYWLWILGTIIEDELGHVSMLVLTIVLAAGSA
                     AAQFAFSVGGIGLSGVGYGLVGFLWIARRTDARFRDAMDARTLQLFVAWGVLCIVLTV
                     TNVLAIGNWAHGAGFLLGIPLAYAFLGRSLAHKLAGALLALILVAACLGAASLWRTRL
                     NLSKDGGDNDFRIGTVELQAKDYDAAIRHLERAIEVSPSDDAAWYNYGVALQNAQGHL
                     GMTKLDAFRKALALAPKEDQYRNAVANELFNQGVDAHEQNDNAAAEKLYRESISIKET
                     GRAVWNLNIVLHEEDRNEEADQLAKRAREIDPDIAKSHPAPDEPADPSKDTPESIDKT
                     GSGSAR"
     gene            11989..13359
                     /locus_tag="VMZ53_09270"
     CDS             11989..13359
                     /locus_tag="VMZ53_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28689.1"
                     /translation="MPRWGLCAVVCVAMPSTAVAQEASAPSSSSFAPEAGLAFDWTTG
                     PAFGDIPTLGGTHGLGVEGRLAAFGVLELDARYELLAIPLPADMGTGLSHQLFGQLKG
                     RWVTDDVRRQHWALGVGYGTAFRPSTLGGRAAIGRVAITRQIGVPSQQVDTAFELAYE
                     RSFGDMHLDMVLGSIRFGYSTGAKAKYSGAKSALFPHTTSFDAFFPWGAGMSFGLHAN
                     QYLSLETSVSYIADVDITSDEVDHHGFRGAHWAAMTGPRVQAGSWPSNEAVLYGQLQG
                     GLGWIARDPGDLRPVQSAELGIRLVCSNWGVELGAWLRTQLADGTLDPIAGGLVLRAI
                     IASDRTVIGGHNRKCADAGTGGSSSPPAETQTISTDTSVGAQLDVGIQAIQEALHPPA
                     ARIEQVQPRAGFVWIAGHWEWRGSSWQWVGGRWEAERANERWVPGTYEVRGGVSIYIE
                     GRWAPR"
     gene            complement(13356..13607)
                     /locus_tag="VMZ53_09275"
     CDS             complement(13356..13607)
                     /locus_tag="VMZ53_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28690.1"
                     /translation="MGRLEDIVERNKNPRKHREGKFPFGIAVSALVLIVLVLVIFTDL
                     GTPKQPNAPAPAPEEKGRVRDIGIYVDRAHRDAGVSVGD"
     gene            13717..17142
                     /locus_tag="VMZ53_09280"
     CDS             13717..17142
                     /locus_tag="VMZ53_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS28691.1"
                     /translation="MIGVRTAEATTFTVTTTQTYNGDGTCDSTCTLYEAVQAANAAPA
                     GPHRIEFAIPGSGVQKIAAELGIATLTAQNVTVDGFTQPGASPGSPKIEIDFFGIGAA
                     GGNWTINGANAVIRGLVINGARTNNAPGTALNINAPNVHVEGCFIGTDATGTAARSNI
                     FGIQIGPNGTNTVIGGPTAAQRNIISGNTFGIDVHATGVSIIGNYFGTDVTGTAAIPN
                     TSFAIEFGAYAAMNMKIGGPSTGERNIISGNGTGISGARTGQPGASGCVLQNNWIGVS
                     ATGVALPNTNGGLVDNVGLFDTVIDNVISGNTNAGGTAVGAQISAFPGASYQFIGNRV
                     GTDPTGTFAVPNDIGVVANIAQIGSSATGLRNVISGNRTVGVRLNAVTDGTLVRGNYI
                     GVGSNGTTPLPNGGAGIESAGATNAGGTITIGGVSQENIIANNLGGGIVVTANRGTSI
                     QANVIQNNTGLAIDLGTAGVTPNDAGDTDTGPNDLQNFPLVTIAPNGMSVSGTLTSTA
                     NRLFSFDVYSNSACDSSGNGEAEAFLGSINALSNGSGVATFTLNVSTPLAAGRFVTAV
                     ARDAGTGQGAGNTSELSACVAVPNADLSVTLNDTADPIDVGGTISWTATVANAGPTSA
                     TGVSLAITLPAGVNVASTTPSQGTCSQTGSVVTCAFGTIANAGNASVTISGTATSGGT
                     LSTSATVTSNEPDPDSGDRTDTESTTVNRPPTATAANVTTNEDTAVGITLAATDPDGN
                     ALTFTVLTNPTKGTLSGTAPNLTFTPNANASGADSFTFRVSDGRLQSAAATISITITA
                     VNDAPVAATDSYTGTENVTLNVVAPGVLANDTDLDSTLTATLVTNALKGTVTLQADGS
                     FTYVPNLDANGTDTFVYKATDGTTSSANTVVTINVSGVNSAPIAITNVYAASEDQALV
                     IGSGMGVLANDSDAEGDSLMASVVSSVGHGTLTLDANGGFTYTPAANYNGTDSFTYHV
                     TDGTLSSADVAVMIMIAGANDLPTAPELIAPADTAQIQLADVELSWQPASDIDGEALM
                     YRVTLNGGGETKTIDTATTAAVLPAAMLSGGAYVWTVQALDAVGPGPTSDTNSFQIID
                     DGDEMQPGEDGGCCDSRSSDGSSSLVLAIGVLLIVGRRRRQSRALRTRSASHV"
     gene            complement(17100..>17314)
                     /locus_tag="VMZ53_09285"
     CDS             complement(17100..>17314)
                     /locus_tag="VMZ53_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HUS28692.1"
                     /translation="QGSEYPAVVIPLVTQHFMMLQRSLLYTAVTRGKKLVVLVGSKRA
                     VQLAVNNADAKKRYTWLAERVRNARD"
BASE COUNT         3173 a         5717 c         5551 g         2873 t
ORIGIN      
        1 cggcgaggat ggcgcgcagg gcaaagactt catcaagacc gacgcatcga tcacgcacgg
       61 caactcaggc ggtcccgtca tcgacgacaa cggccggctc gtcgggattg cggcggcatt
      121 tcgcacgcgc atgactgcaa ccggcggcgt gatcgaaacc gcgcaggtcg gcctcgtgcg
      181 ccccctccaa gcggcgagca acttgctcgc gtacgcagcg gcaggctgga ctccgcgcga
      241 gggttacacc gacgtcgagc tcacgccgac ggcgatcgaa gccgccgccg agggcgtgcg
      301 catctacacg acggtcgtcg atgacgcgaa cgattcaccg gtgcgtgacg cgacggtgat
      361 ggtgctcaag gccggcgtca acacgacgac gatcgacatg aatcgcgtcg acgatcaggc
      421 gatcgcatgg ggcaagacga acgcgctcgg cgaagtgcgg ctgaagcagc tcgtgccgat
      481 tccgggcacg tacaccgtga tggtcatcgc acctggctac gagccgctca tcggcgagag
      541 cgagctgcat ctcgacgaca agacgccgcc gagcttcgat ccttggggca agatcggcct
      601 gcgcagccga tagctcagct cagaagctcg tcgatcgcat cgctgaagga cttcgacgtg
      661 aacggcttct cgagaaatgc atcgggccca tcgtcaccgc ctacgctgcg ctcgacctcg
      721 gtgtagccag acatcaggag cacgcgcgca tcgggattcg tctctcgcgc gattggtagc
      781 agctggtagc cggtgagccc gggcatcatc acgtcgagca cgatgaacgc gtacgtgccc
      841 tcgcgcaggc gagcctccgc ctccgtgccg ctcgcggcgg tgacgacggt tgcgttgcgg
      901 cgctcgagca agcggcggat cgtcgtgcgc accgcgggtt cgtcgtcggc aatgagggcg
      961 cgcatgcccg caagtgaaat gccgtgtgcc gtctgcttcg gtgcacgccg tggcttcact
     1021 tcgccagccg cgagcggaaa ccgcagcgtg aacaacgagc cattcgcggg ccgtgactcg
     1081 acggtgatct ctccgctgag cctgcgcacg atcccgacga ccgccgagag gccaagccca
     1141 tcgtgaccac gcttcgtcgt gaaaaacgga tcccagattc gcgcgacggt atccggatcc
     1201 atacctgtgc cgcggtcgcg aatctcgatc tgccagcggc cagacgctgc gctcgtcgtg
     1261 atctcgatcg gagcttgcga ctgcgcttcc gcggcgttcg cgacgaggtt tgcgagcacc
     1321 tgcacgagga gggcgcgatc cgcttcgatc accacgcgtc cagcgccggt cgtgatcgcg
     1381 aggtccaccg gcttcgcgag ctgcgcgagc tgatcgcgtg catcgcgaac gagctgatcg
     1441 gggtcgagga gctcgctgac gaaccgccct cgaccggcat acgcgagtaa ctggcgcgtc
     1501 agctgcgcca tgcggttcgc agccgcctcg atgcgcgtca acgactcggc ggtcgcctcg
     1561 gagagctggc cgctctcgcg tgctgcactc gcttcgacga gcacgctcgt cagttgattg
     1621 ttgaagtcgt gcgcgatgcc gccggcgacg aggccgagcg cctcgacacg ctgcttcgca
     1681 tgaacctctg cggcgcgtgc cgcctcgacc gagggatcgc gctcgacgcc gaggacgagg
     1741 aagaacgtgc gctccccttc cgtccgcagc accgcgaggt agtggatcga ccgcgggccg
     1801 tgcggcgtcg cgagcgtgat atcggcccgg tactgaccgc gtgcgcgcgc gttgcgcacg
     1861 agctcgggcc acacatgctc accgcctggc gcgaactccc aggctttctt gccgacgatg
     1921 tcggcggcgg catgcccgtg gagaagagcg gccgcctcgt tgaggcgcgc gatcgtgccg
     1981 tcgagctcga agacccacgc tgggacgggg agctggtcga aatcgagggc caaggacgag
     2041 aggggcacgt acaacttccc ggttcggccc ccttggtacc gcgttcagat gcggctcggg
     2101 tacgtgcgat aacaactcaa taggtctgca tacttgccgg ttgtttgcgg agcgtggtac
     2161 ggctcatttc gttccatgcc gtctctggac cgcactgcga tcgatgccga tgccgatcgc
     2221 gtggtccgca agctcacccg cgcgggctac aaggcgtacc tcgtcggagg ctgcgttcgc
     2281 gatcttctcg tcgggcgaac cccgaaagac ttcgacgtcg cgacgagcgc aacgcccaac
     2341 gagatcaagg cgacgtttcg caactcgcgg atcatcggcc gccggtttcg gctcgcgcac
     2401 gtgttcttcg gctcgaagat catcgagacc gcgacatttc gagcgaaccc gcgcgacgaa
     2461 gacgatcacg atctactgat ccgtcgcgac aacgtgttcg gcaccgaggc cgaagacgcc
     2521 aagcgccgcg acttcacgat caacggcttg ttctacgacg tcgagcgcga ggaagtgatc
     2581 gaccacgtcg gcggtctcgc tgatctcgat gcacgcctcg ttcgcacgat cggcgatccg
     2641 gatatccgct tccaagagga tccggttcgc atgctgcgcg cgatcaagtt cgcggcgcgc
     2701 ctgtcgtttg gcttcgagcc agcgacctgg cgtgcgctcc tgcgctggcg ctccgagatc
     2761 agcaagtgcg cacctcctcg cttgctcgag gagattcacc gcctactccg cggtggcgcg
     2821 gcgcgacgct cgttcgagct gatggtcgag accggcgtgc tcgccgtgct gtcgccgtat
     2881 ctcgcgggct tgctcgaagg caccggcgca ccgacaacgg ccccgccgat cgccgacacg
     2941 gcgcctcgcc cgaccgtgcc gcgcgtcgag tatgacgacg aggatccagc ggagcacgat
     3001 cccgacgatc ccgatgagga agagggctcg ctgcccgtca acggtgagcc ggaagccgcg
     3061 cccgccgaag agacggaaga gctcgacgac gacgaacgcg agtggcatcg cgtgtgggcc
     3121 gacgagccgc ccgcgcgtcc cgcgcatgcg atggcgatca agctgtcgtt tctgtcggcg
     3181 ccggagatgg cgaagcgtcg cgcgctcgca tgggaagcgc tcggccagct cgatgccgcg
     3241 gtcgccgatg gtcgcgatcc gagcaacgcc gtcgcgttcg gcctcctgct cgcggcgttc
     3301 ctccccgagg aattgcgcgg cggcgagctg cacggcgcgc tcaacgagct cgcgcatccg
     3361 ctcatccagc agctgcacgt cacgcgtcgt gacagcgagc gtctgaagta catcctcatc
     3421 gcgcagcgca agctggccgc agcgaagaag cgcggcgtcc aagccgagct cgccggcggt
     3481 cgcgagctga tcgacgacgc gctgctgctc tacgcgctgc tcgagcgcgc cggcggcaac
     3541 gagtccgcca tcccgcctgc tgcgatcgcg acgggtgaag gccgcgatga cgaagaagac
     3601 agcgacgatc cgaatcgccc gcgcaaacgc cgtcgccgcc gtcgaggtgg ccgtcgccgt
     3661 ggcggtaacg ccgcgaacgc gtgatgcccc ggctccccgc tggcctcatt gaagatctag
     3721 tcgatgccgg ctcgcgcgag cactacgccg acgccgcgct gtacgactac gagtacaggc
     3781 gccgccgcgc agatgtcgcg ttctatcgcg agctcgcgaa gcagcgcctc ggcgatccgg
     3841 cgaagctgaa gcccggcagc gcgcgcatcc tcgagctcgg tgcaggctcg ggccgtatca
     3901 cggtgcagct cgcacgcgcc ggctacgagg tcgtcgctgt cgatgcatcg gcagcgatgc
     3961 tctcgaagct gcgcgatcgc gtcgccgcac tgccgcaggc cgtcgcgaag cggatcaccg
     4021 tcgtgcaggg cgacctgcgt gacttcgcga tcgacggcaa gaagttcccg ctcgcgatcg
     4081 cggtcttcaa cgtgatcgag cacctctata cgcgcgtcga agtcgatgcg tgcttgcgtc
     4141 gcgtcgtcga gcaccttgcg ccgaacggtg cgttcgcatt cgacgtccag cttccggacc
     4201 tcgcgtggct cattcgcgat cccgagaagc ggtgggcacg cacccgcttc accgatccga
     4261 cgacgggtcg ggcgatgctc tattccacga accacgacta cgatccggtc ggccagatcg
     4321 tgctcatccg gctctattac gagcccgtcg acggaaaggg ccccacgaag atcgtgaagc
     4381 tctcgcagcg aaagttcttc ccggcggagc tcgaagcgct cgtcgcgcac gccggactgc
     4441 gcatggtgga gcgttatggc gattttggct ggcaggccct acaccccgcc gccgagagcc
     4501 aagttctcgt ctgtgaacga gccatttcgc gcaaatcctc gtcgaggaat tcccgtttct
     4561 cgtcgaggat ttagatcacg cgagatcctc gtcgagaaac aggttatcca cagcttttcc
     4621 tcgtcgagaa atcgtccccc gggatcactt ctccgacgag aaaaatcgat cttttgatca
     4681 attcctcgtc ggtttcggga gtgtttgtca gacggttcgt gtagcttgcg cgcacccaat
     4741 tatccctagg gagggtctga cactatgggt gagaagattg cgaagctcgg aatcaagcgt
     4801 gacaacgaca tgatgtacta catcaagggc ggcgacgtgt gggcgacccc acgcaagaag
     4861 cccggccagc cgaagggcaa ggctgcgaag gtagcggctg ctggcatcga catggattac
     4921 agcaagttcc tctactacct cgacggtgac ggcgacgtcg cccgcaagga gcgccaggtc
     4981 ggtggctcca agcgcaagag cagcggaaag aagtcggcgg cgaagaagcc ggcgaagaag
     5041 accgccgcga agaagaccgc caagaagggc gccgcgaaga agagcggcgg caagaagggc
     5101 aagcgcaagt aagcgctgcc tggactgttg gtctgattac ttcgtgcgcg tcgcgcacgc
     5161 gattcactga ctagactcta attaacgctg gaagggctcg caggactgcc gatgactcgg
     5221 cggcaccgcg ggcccttcga gtttttcggc cgcgcgtttt tgggcgtaca gccgcctctc
     5281 gccgcaggtt tgctgtagca tttcaccgag gtggtgatgc ttcgcctgct ttcgagaggg
     5341 ctcctcgtag gtgccctcgt tcttgggtac accgctcatc acgagcgtct ggcgtatgcc
     5401 gacgatgatg aggaagaaga agaggacgac ggcggcggcg acaagggcgg cgacggcgag
     5461 ggcgacgacg aggagggcga gggcgaaggc gacgacgaca aggaccagcc tcccgtcaca
     5521 gccggcggcc tcttcacgat gacgtcgtat ccggtacgcg aaatctcgcg gccgctgacg
     5581 ctcacgcagg ggatcacgca gctgcgcgca tcggccggta cggatctcag cgcgaagggc
     5641 gcgttcgaga gcggaggcct gtcgctcgaa gcggtctacg gccaagccga caacttcaac
     5701 atcattggtg gcctcaccaa cgcgtacaac ttcaaacaat tcggcttcta tgccgggttc
     5761 gatgcggcgc tcgcgtacga catcgtcgac ttccggctcg ccgccaacgt tcatcgcaac
     5821 gcgctgccgg tgtacgcgga cttctgcaac ccgcccgcgt cgccgaacga aatgccgaac
     5881 ggcatgggca tgtgctcgga tccggcgacg cagcaggtgc aggcgttgcc gagcggtgat
     5941 tacctcgcag gaagcacgca gttctcggtc gacatcggtt ttccgttccg ctacgcgatc
     6001 aagcccgagg tcgcgatcat cgcgctctac acgctgatgc agatcgactt caactcggcg
     6061 aagacggatc acgtgaccct cagcgtcgag gatgacccga acaaccccgg caagaagaag
     6121 gtccgctcgg cgaacaccgg cgacggctac aagcccgacc tcaacccgtc ggttggcatc
     6181 gcgacgaacc cgctcccgcc gctgtccgtc gtgatcttcg cgcagctgcg cattcccgag
     6241 ttcgacacga gcgccggcgc gttccaggtg cccgtcaccg gccgcatcga gttctcgccg
     6301 aaccagaagt tcgatctcgg cctcgagttc acgctgctca acgtcaagcc gcccgagggc
     6361 cagagcccga tcgacaaccg cttcctcgcg ctcttcattc agtcgaggtt tggtaagtga
     6421 aagtctgtcc gcgttgcttc acgcgctttc ccgacggaga gcgattctgc ctccacgact
     6481 ccgcggtcct ggtggaagag gaagacatcg cgcgcctcgg ccacaacatc ggcaactacc
     6541 ggctcgataa gatcctcggc cgcggcggca tgggcaccgt ctatttcggc gagcacgtct
     6601 acatcaagaa gcccgtcgcg gtgaaagtgc tgcatccgca gttcgctcgc tatcaggaag
     6661 cgattcaccg cttcttgcgc gaagcgcgtg cggcgacgtc gatcaatcac ccgaacatcg
     6721 tcgacgtcac ggacttcgga attctccccg aaggccccgt ctatttcgtg atggagttcc
     6781 tcgagggcaa gtcgctcgag gatttgatcg agaaggacgg cgccgtcgag ctgcatcgcg
     6841 cgctgaacat cgcgaaccag atcgcgctcg cgctcgatgc ggcgcacgcg gtcggcgtca
     6901 tccatcgcga tctcaagccc gacaacatca tgctgctgaa gaaacccggt cgacgcgaca
     6961 tcgttcgcat gacgccggac cagcagtgga tcaccgagcg cgaggaatcg tacgacttcg
     7021 tgaaggtcct cgatttcggt atcgccaaga cgctcatcca agacgagctg atggcggaga
     7081 cggtgcaagg cgccgtgttc ggaacgcccg agtacatgtc gccggaagct gcgcgcggcg
     7141 aggacgtcga ccatcgcgcg gatgtctatt cgctcggcgt catactgttc gacatgctct
     7201 gcgggcgtcc gccattcgag gctccgcagt cgagcgaagt gctggcgatg cacatccatc
     7261 agccgccccc ttcgccccgc gagtttgcac ctcaccgtga aattaccgag caggccgagg
     7321 gcgtgatcct gaaagcgatg caaaaagatc cgaagcagcg ctatcaagac atggtcgagt
     7381 ttcggcgtga cctcgaaatt tcctatggct cgatcagcta ccgacgccgt ggtgcgcctg
     7441 tgcgcggtct cgagccacgc ggcctcgaag cacgcgctcc aaagaagcgc ctgaccgagg
     7501 agctcgacga gtggatcacg aacgatccat cgggcatgtc cgtcgagcag gcgcgcatgg
     7561 tctcgcttgt cgagacggca gagaaagcgt tcacgccaag tggcatgtcg cctgcggaag
     7621 cagagcgcat cgcgaacgct ctcgatcgcg cgctcgacga cgacgagtaa cgctgacgcc
     7681 cgtcggcaac tcgacggtaa cgcttgccgc gtgctagctt gcgctcgtgt ctaaccaagc
     7741 cagccagtct gggcagatta ctctccggaa ggacgtcaag ttcaacggag tgaaggtctt
     7801 ctcggcaacc atggtggcgg accgggatca gttgggcgag aaggtgaccg cctggattgc
     7861 tgcgaacaag gaacataaaa aggtcacgga catcgtgatc acacagtcgt cggacgaggc
     7921 atttcactgc atcgcgatca ctgtctttta ctgggaagag cggtcctgac gaccaggaag
     7981 ctcaatttct cgtcggggcc agcagttctc ccggaagagg ttctcgagaa ggcgcaagcc
     8041 gccattctcg atctcgacgg ctctggcatc gggattctcg aacattcgca tcgcggcaag
     8101 gagttcggcc gcgtgctcga ccgtgcggaa gcggcggtgc gccaggtcgg cagcattggc
     8161 gacgactacg ccgtgctgtt cgtaaccggc ggcgcgacgc aacacttcgc gatgatcccg
     8221 cagaactttc tcggcgcggg cgagacggca gactactgcc acaccggtat ctggtcgggc
     8281 aaagcgatcg atgaagcaaa gcgcgtcggc atggtgcacg tcgcgtgcga tgggaagccg
     8341 gacaacttcg cgagcattcc atcgccgctg aattggtcga agcagccgcg ctacgtccac
     8401 tacacgagca acgaaacgat ctacggcacg cagtggccgg cgccgccgga tgcgcctgcg
     8461 ccgctcgtct gcgacgcatc gagcgacatc ttcagcaggc cgctctcgct cgcgaaccac
     8521 gcgctgatct acgtgggcgc gcagaagaac ctgggcccca gcggcatctc actcgtgatc
     8581 gcgcgcaaag acttcgtcga gacgggcgaa cgtcatcttt cgccgctcgc gcagtaccgc
     8641 acgtacgtcg ccgagcgttc gatgcacaac acgccgaaca cgtttggcat ctggatgatc
     8701 gccgaaacgt gcgaatggat cctgcgccaa ggtggcctcg tcgcgatggc cgagcgcaac
     8761 ctccgcaagg cgaagctgct ctacgacgcg ctcgacgcga gcaaggtgtg gcgcccgcac
     8821 gcgaaggccg gcagccgctc gctcatgaac gtgacgttcc gcggtgcgac gcccgagctc
     8881 gaagacgcct ttatcaagcg cgccgaggag cgcggcatga gcggtctgcg gggccatcgc
     8941 tctgttggcg gcatgcgcgc cagcatctac aacgcttttc ccgaggatgg cgttcgcaag
     9001 ctcgtcgagc tgctccacga gatggagccg tcgtgatatc cctgcgttga gtgacgttcc
     9061 tcaccgacga aggcgagaag gagctggagg cggcggtcgc tgcgatcgaa gcggtgtcgt
     9121 cggcggagat cgtcgtcgcg atccggccgc gggtgcgtgg ctcgatcgtg cagcacgtga
     9181 tcgtcgcgct cgtcatgagc atcggcatgc tcgcattcac gctctacagc ccgatcgaat
     9241 tcaggctgtg gcacatcctc acgctgccgg tcatggctgg gctcgcgggc gggttgctcg
     9301 tcgaggccgt ccccgcgctg taccgcttct tgctccccgc gcagctgcgg agcgtgcacg
     9361 tctacgacgc ggcgtgtgct gcgttcgtgc aacggggcat tcacgcgacg cgcgatcgca
     9421 cgggcatcct cgtgtacgtc gcgcttcgcg agcagtgcgt cgagctcgtc ggcgatctcg
     9481 gtgtgcgcga gcacgtcggc gccgaaacgc tgatcgcgtg gtcgggcatc ctcgagggcg
     9541 ccctgccgaa cggcgcaaag gccgtcgcga aagagctcgc agcactcgcg ccggagctgg
     9601 cgaaggccct gccccgccgc gccgacgaca tcgacgagct cgcgaacggc gtgcagctcc
     9661 tctccgctcg cccgcgccag caaggcaagg ctgcccgatg acgatgccga agcgcgtcgt
     9721 gcttcttctc ctgctcgcga cggcgactgc atacgcacgt ccgggtagca gtggcacctc
     9781 gaccacctat cacccgagct cgacgtatca ctacaacggc tcgcatacgc gaggcagcgg
     9841 cgacctcggt gcgggcggcg tgatcgcgat cttcctcggc gtcttgatct tcgtcggtct
     9901 gtggtccgca atcgcgcacg cgcggcaagg ctcgcgcacg gtgcaggtca caacgccgcc
     9961 aacgccgcgc ctcgacttca cgccgctgcg caccgccaac gcgagcttct ctcgcttctc
    10021 gtttgaagac accgtcgccg agctagtgcg cgccgagctc gcgcgtcgtc acgaagcgaa
    10081 gcccgaggcg gtgctcgtcg ccgacatccg gcccagcgcc gccacgagca cgcatgtcac
    10141 gtgcactgtc gaagcgacga tcggtggcac gtacaacgtc ggccactgga cgttcgagcg
    10201 caccgctgac aatacgtggc gcgtcgacgt ctatcgcgac atctcgctcg gccgcggtcc
    10261 ccttctcatc tcgacgggtc gcgaggtcgg caacgatctc cctacgctca ccgacgagca
    10321 agcgatcgtc cgcttcacgc agatccgcga cgaggacaag acgacgagct gggacgcgtt
    10381 caccgcacgc gtgcgcgaga cgtacgcaaa gctcaacgac gcgtggaaca agcaagagct
    10441 cgccgacgtc gacggctacg tcacgccgcg catgatcgag tggctgcgct tctggtacgc
    10501 cgagtaccgc cgccaaggtc tcgtcaatca tcacgccgac gcagaagtcc gccacatcgg
    10561 cctcgcgcaa gtcacgcgtg agccaaacct cgatgcggtc accgtgcgcg tctacgccgc
    10621 gggcttcgac ttcacgacga aggcgaacac gacggacaag gtcgcgggct cgcagaccga
    10681 gaagcgcgag tacaccgagt actggacgtt cgtgcgcgca aagggctcgc cgagctggct
    10741 gctcacgaag atcgagcaag acgagctgta tctcggttag cgatgcgccc gcctccgtct
    10801 atcgcgcgct tcgtttcgtt cccggtcgtc gcgatgatct cgctcgccgc gatgttcgtg
    10861 accggcgccg tcatgttcgg aaagaccgag atcgagccgt tcatcatggg ctcgatcgca
    10921 ttcgagactc aaccgtggcg tctcgcctcg agcgcgctgc cacacgcggg cccgatccac
    10981 ctcctcttca atgtctactg gctctggatc ctcgggacga tcatcgagga cgagctcggc
    11041 cacgtctcga tgctcgtact caccattgtc ctcgcggcgg gctcggcggc cgcgcagttc
    11101 gcattctccg tcggtggcat cgggctctcc ggcgtgggct acggcctcgt cggcttcctg
    11161 tggatcgcgc gccgcaccga cgcccgcttc cgcgacgcga tggacgcgcg gacgctgcag
    11221 ctgttcgtcg cgtggggcgt gctctgcatc gtgctcacgg tcacgaacgt cctagcgatc
    11281 ggcaactggg cacacggcgc cggcttcctc ctcgggattc cgctcgcgta cgcgttcctc
    11341 ggtcgctcgc tcgcgcacaa gctcgccggc gcgttgctcg cgctgattct cgtcgctgcg
    11401 tgcctcggcg cggcatcgct ctggcgcact cgcctcaacc tgtcgaagga tggcggcgac
    11461 aacgacttcc gcatcggcac cgtcgagctg caagcgaaag actacgacgc cgccatccgc
    11521 cacctcgagc gcgcgatcga ggtctcgccg agcgacgatg ccgcttggta caactacggc
    11581 gtcgcgctgc agaacgcgca aggccacctc ggcatgacga agctcgacgc gttccgcaaa
    11641 gcgctcgcgc tcgcgccaaa agaagaccaa tacagaaacg ccgtcgccaa cgagctgttc
    11701 aaccaaggcg tcgatgccca cgagcagaac gacaacgccg ccgccgagaa gctctaccgc
    11761 gagtcgatca gcatcaaaga aacgggccgc gcggtctgga acctcaacat cgtgctccac
    11821 gaagaggacc gcaatgaaga agcggaccag ctagcgaagc gcgcacgcga aatcgatccc
    11881 gacatcgcaa agagccaccc agcgcctgac gagccggccg acccttccaa ggacacgccc
    11941 gaatcgatcg acaagacggg ctcgggttcg gcgcgctaag ctctaacgat gccgaggtgg
    12001 gggctgtgcg ccgtggtttg cgttgcgatg ccttcgacgg cagtcgccca ggaagcatcg
    12061 gcaccgagca gcagctcgtt cgccccggaa gccgggctcg cattcgattg gaccaccggc
    12121 ccagcatttg gcgacatccc gacgctcggc ggcacgcacg gcctcggcgt cgagggcagg
    12181 ctcgccgcat tcggtgtgct cgagctcgac gcgcggtacg agctcctcgc gattccactg
    12241 cccgccgaca tgggcacggg cctcagccac cagctcttcg gtcaactgaa aggccgctgg
    12301 gtcaccgacg acgtccgccg ccagcactgg gcacttggcg tcggctacgg cacggcgttt
    12361 cgaccgtcga cgctcggtgg tcgcgctgcg atcggccgcg tcgcgatcac tcgccagatc
    12421 ggtgtcccgt cacagcaagt cgacactgcc ttcgagctcg cgtacgaacg ctccttcggc
    12481 gacatgcacc tggacatggt gctcggcagc attcggttcg gctactcgac cggcgcaaag
    12541 gcgaagtact cgggcgcgaa gtccgcgctg tttccgcaca cgacgtcgtt cgacgcgttc
    12601 ttcccctggg gcgccggcat gagcttcggc ctgcacgcga atcagtacct cagcctcgag
    12661 acgagcgtca gctacatcgc cgacgtcgac atcaccagtg acgaggtcga tcaccacgga
    12721 tttcgcggag cacactgggc cgcgatgacg ggcccacgcg ttcaagcggg cagctggccc
    12781 tcgaatgaag cggtcctcta cggccaactg caaggtggcc tcggttggat cgcacgcgac
    12841 cccggcgacc tccgccccgt gcaatccgcg gagctcggca ttcgactcgt ctgcagcaac
    12901 tggggcgtcg agctcggcgc ctggctacgc acgcagctcg ccgacggcac gctcgatccc
    12961 atcgcgggcg gtctcgtgct ccgtgcgatc atcgcatccg accgcaccgt gatcggcggg
    13021 cacaatcgca agtgcgccga cgcaggcacg ggcggcagca gctcaccgcc agctgagacg
    13081 cagacgatct cgacggacac gagcgtcggc gcccagctcg acgtcggcat tcaggcgatc
    13141 caagaggcgt tgcatccgcc cgccgcgcgc atcgagcagg tgcaaccgcg cgcaggcttc
    13201 gtctggatcg cgggccactg ggaatggcgc ggaagctcgt ggcagtgggt cggtggtcga
    13261 tgggaagccg agcgcgcgaa cgagcgttgg gtgcccggta cgtacgaagt gcgcggcggc
    13321 gtgtcgatct acatcgaagg tcgctgggcg ccgcgctaat cgccgacgct gacgcccgcg
    13381 tcacgatgcg cgcgatcgac gtagatgccg atgtcgcgca cgcggccctt ctcctcgggc
    13441 gcaggcgcgg gcgcgttcgg ctgcttcggt gtgccgaggt cggtgaagat gacgagcacg
    13501 aggacgatca atacgagagc tgacactgcg atgccgaagg ggaacttgcc ttcgcggtgc
    13561 ttccgcgggt tcttgttgcg ctcgacgatg tcttcgaggc gacccattcg agtgaactat
    13621 tgcaccttcc gactcggtgc gccctcgttg taaggtggcg ctttcgacac gggggtgtgt
    13681 atgcgtagct gcgtcttctt agggttgttg gtcgcgttga tcggggtccg cacggccgaa
    13741 gcgacgacgt ttacggtgac gacgacgcaa acgtacaacg gcgacggcac ctgcgattcg
    13801 acctgtacgc tctacgaggc agtgcaggct gcaaatgcag ctccggccgg cccgcatcgt
    13861 atcgagttcg cgattcccgg aagcggcgtt caaaagatcg cagccgagct cggcatcgcg
    13921 acgctgactg cccagaatgt cacggtcgac gggttcaccc agccaggtgc atcacccggc
    13981 tcgcccaaga tcgagatcga cttctttggc atcggagcgg ccggcggaaa ctggacgatc
    14041 aacggcgcca atgcggtcat ccgcggcctc gtcataaacg gcgcgcgaac aaataatgcg
    14101 cccggcacgg cgctgaacat caacgcgccc aacgttcacg tcgagggttg tttcatcggt
    14161 acggacgcga ccggaacggc ggcgcgaagc aacatcttcg ggatccagat cggccccaac
    14221 gggacgaaca ccgtgatcgg cggcccgacc gcagctcaac ggaacatcat ctccggcaac
    14281 acgttcggca tcgacgtgca cgccacgggc gtctccatca tcgggaacta cttcgggaca
    14341 gatgtcacgg gaacggcggc gatccccaac accagcttcg cgatcgagtt cggcgcgtac
    14401 gccgcgatga acatgaagat cggcgggccg agcaccggtg aacgaaacat catttcgggc
    14461 aacggcaccg gcatctcagg cgccagaacc ggccagcccg gcgcgagcgg ttgcgttctc
    14521 cagaacaatt ggatcggcgt ctcggcaacg ggcgtcgccc tgccaaatac aaatggcggt
    14581 ctcgtcgaca acgtcggcct cttcgacacg gttatcgaca acgtgatctc cgggaacacc
    14641 aacgccggcg gcactgcggt cggcgcccag atcagcgcgt tccccggcgc gagctatcag
    14701 ttcatcggca accgcgtggg caccgatccg actggaacgt tcgccgtccc gaacgacatc
    14761 ggtgtggtcg cgaacatcgc gcagatcggt agctccgcca ccggcctccg caatgtcatc
    14821 tctggcaacc gtaccgtcgg tgtccggctc aacgcagtca ccgacggcac actcgtgcgt
    14881 ggcaactaca tcggcgtcgg gagcaatggc accacgccac ttccgaatgg cggcgccggc
    14941 atcgagagcg cgggcgcaac aaacgcaggc ggcacgatca ccatcggcgg cgtgagccag
    15001 gagaacatca tcgcgaacaa tctcggcggc ggcatcgtcg tgactgcgaa tcgcggcacg
    15061 tcgatccagg cgaacgtgat ccagaacaac accggactcg cgatcgatct cggcactgcg
    15121 ggcgtcactc cgaacgacgc gggcgatacc gacaccggac cgaacgacct ccagaacttt
    15181 ccactcgtca ccatcgcgcc gaacggaatg tcggtgtcgg gtacgctcac ctcgacggcc
    15241 aatcgtctgt tctcgttcga cgtctattcg aacagcgcgt gcgattcgag cggcaacggt
    15301 gaagcggagg cgttccttgg ctcgatcaat gcgctttcga acggctccgg cgtggcaacg
    15361 ttcacgttga atgtgtcgac gccgctcgcc gcaggacgtt tcgtcaccgc ggttgcacgc
    15421 gatgcaggaa ctggccaagg cgccggcaac acctcggagc tctccgcgtg tgtcgctgtc
    15481 ccgaatgccg acctctccgt gacgctgaac gacaccgcgg atcccatcga tgtcggcggc
    15541 acgatcagct ggactgcgac cgtggcgaat gctgggccga cgtcggcgac gggcgtgtcg
    15601 ctggcgatca cgcttccggc gggcgtcaac gtcgcgagta cgacgccgtc gcagggaact
    15661 tgtagccaga ccggcagcgt cgtgacctgc gcgttcggca cgatcgcgaa tgccggcaat
    15721 gcgtcggtga cgatcagcgg tacagcgacg agcggcggca cgctcagcac gtcggcgacc
    15781 gtcacctcca acgagcccga tcccgactcc ggcgatcgca cggacaccga atcgacgacc
    15841 gtcaaccggc cacccacggc gaccgccgcg aatgtgacga cgaatgagga tacggcggtg
    15901 ggtatcacgc tcgctgcgac ggacccggat ggcaatgcgc tgacgttcac cgtgcttacg
    15961 aatccgacga aaggcacgct gagcggaacc gcgccgaacc tgacattcac gccgaatgcg
    16021 aatgcaagtg gcgcggactc gttcacgttc cgcgtttctg atggtcgctt gcagtctgcc
    16081 gcggcgacga tctcgatcac gatcaccgcg gtcaacgatg cgccggttgc cgcgaccgac
    16141 agctacaccg gcaccgagaa cgtcacgctg aatgtcgtcg cgcccggcgt gctcgcgaac
    16201 gataccgatc tcgactcgac actcaccgca acgctcgtta cgaacgcgct caagggcacc
    16261 gtcacgctgc aggccgacgg gtcgttcacg tacgtgccga acctcgacgc gaatgggacc
    16321 gacacgttcg tgtacaaggc gaccgacggc acgacaagct cggcgaatac ggtggttacg
    16381 atcaatgtct cgggcgtcaa cagcgcaccg atcgcaatca cgaacgtgta tgccgcgagc
    16441 gaagatcagg cgctcgtgat cggcagcggg atgggtgtgc tggcgaacga tagcgatgca
    16501 gaaggtgact cgctcatggc atcggtcgtc agcagcgttg gacatggcac gctgacactc
    16561 gatgcgaacg gcggcttcac gtacacgccc gccgcgaact acaacgggac ggacagcttc
    16621 acgtatcacg tgaccgacgg cacgctgagc tccgcggatg tcgctgtcat gatcatgatc
    16681 gcgggcgcga acgatttgcc gaccgcaccc gagctcatcg cacccgcgga tacggcgcag
    16741 atccagctcg ccgatgtgga gctgtcgtgg cagccggcca gcgatattga cggcgaggcg
    16801 ctgatgtacc gcgtcacgct caacggcggc ggcgagacga agacgatcga caccgcgacg
    16861 actgcggctg tgcttcctgc ggcgatgttg agcggcggcg cgtatgtctg gacggtgcag
    16921 gccctcgacg ccgttggacc gggcccgacc tcggacacga attcgttcca gatcatcgac
    16981 gacggcgatg agatgcagcc cggcgaggac ggcggctgct gcgattcgcg ctcgagcgat
    17041 ggcagctcct cgctcgtgct cgcgattggc gtgctgctga ttgtcgggcg acggcgccgt
    17101 cagtcccgcg cgttgcgcac gcgttctgcg agccacgtgt agcgcttctt cgcgtcggcg
    17161 ttgttcacgg cgagctggac cgcgcgcttg ctgccgacga ggacgacgag cttcttgccg
    17221 cgcgtgaccg cggtgtagag caggctgcgt tgcagcatca tgaagtgctg cgtgacgagc
    17281 gggatcacga cggcggggta ctcggagcct tggc
//
LOCUS       DATLBZ010000177         6907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_171903, whole genome shotgun sequence.
ACCESSION   DATLBZ010000177 DATLBZ010000000
VERSION     DATLBZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171903"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VMZ53_09290"
     CDS             complement(<1..926)
                     /locus_tag="VMZ53_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HUS28693.1"
                     /translation="MRIAVALVLPLLACTSSSSTVATGELGEWHVAAAMPTPRANHCS
                     AAIGDWVLVIGGNYMASDGFVKTDEIHAAKLSPDGTLGNWQLAGRTPSPVTECNATSD
                     ADSLFIVDGIYDREEDSRKVWTATLDDSGMLGALSPMGTLPDIAISTEAAVRNGELIV
                     MKTKVPANDSGQTVALTSPVASLTWSTHDLQIGFRAQAEYAFSDHFIYTVGGYHDTTV
                     GVLPDVSLSPLAGGAAIATTSLPQPVGFGEATVVDEWLFVVGGRAQVFGAPGTTQVYA
                     APLAADGTVGAWTSTLALPMARTNHDLVLAGD"
     gene            complement(1008..1448)
                     /locus_tag="VMZ53_09295"
     CDS             complement(1008..1448)
                     /locus_tag="VMZ53_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS28694.1"
                     /translation="MLRTAILVVAVLLPAIAHAEFATPRDTGDVRPAATIKRTKVERS
                     PSRVALVTTPRLTVDEVLTKINTIYMSGLQRCYRKSLAADPSLSGKVVLAFQVDADGH
                     ALSDITGGRKFDECLSHIVAHWRFSPPANQNDASFRISLALQNN"
     gene            complement(1603..2445)
                     /locus_tag="VMZ53_09300"
     CDS             complement(1603..2445)
                     /locus_tag="VMZ53_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS28695.1"
                     /translation="MKIRFWGVRGSIPAPGPETNRYGGNTSCVSITTAKGDLVIIDMG
                     TGLMHLGNTLMGQAFGKGKGHAAILLSHTHWDHIQGLGFFPPVFIPGNQFTVWGPGGS
                     PEVLEEILEGQMDPNFSPLQTLKNLAATFDVCAITCGQPFEAEGLRVVAREHPHGATT
                     ALSFRIQEGNRTFVYASDVGYPESGPPADAIAHYHGADVLLHDSTYRPDDQVTRRNRG
                     FSSYEDACAAAIAAKVKTLVTFHYDQDYSDDDVDQIVAHCKELLAPHGIGVVPAREGD
                     ELEI"
     gene            complement(2442..3359)
                     /locus_tag="VMZ53_09305"
     CDS             complement(2442..3359)
                     /locus_tag="VMZ53_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS28696.1"
                     /translation="MKVRFWGVRGSIPVPGRATNRYGGNTSCVEVRPKNGSPIIIDAG
                     TGIRRLGKSLMEDSFGDGRGDAFVLISHTHWDHVQGLPFFSPLYRAGNKIHIFARQRD
                     LHLEAVFSQQHNAPYFPVPLSAMHAEMKFHELVEGATFELGTAKVTCARLNHPWVAIA
                     YRVDVDGASVVYCSDTAPFTDMLLGRDFIERPSFGTLPPPIQDELKSMRAGVVALCKN
                     ADLLIYDTQFTPDEYKVRPHWGHSRPDDAIEIAREAKAKKLCLFHHAPLRSDDENDAI
                     LAQYREVTAAEKFDLVSAYEGLEIPLGDE"
     gene            complement(3401..3853)
                     /gene="dtd"
                     /locus_tag="VMZ53_09310"
     CDS             complement(3401..3853)
                     /gene="dtd"
                     /locus_tag="VMZ53_09310"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HUS28697.1"
                     /translation="MRAVVQRVSRASVTVDTQITGSIDRGLLVFLGAGAGDGAADLQY
                     VVDKIVNLRIFPDDAGKMNRSVLDIVGGVLVVSQFTLYGDARQGRRPAFTGALEPVAA
                     KALYEQSLDAIRAAGVAKVEAGEFAADMKVELLNDGPVTILLDSRKLF"
     gene            3923..4399
                     /locus_tag="VMZ53_09315"
     CDS             3923..4399
                     /locus_tag="VMZ53_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28698.1"
                     /translation="MRYLVLAIAVATLSAGCGKEIGDSCVVSSDCSPNGDRQCDVSQK
                     EGYCTVMGCDFSTCPDEAVCVRFFTGGFGNRTCESSPDECSLDELCDLNKRCVPRSSE
                     VRYCMKTCDSNDDCRDGYECRDLAKMKSHGGEPVLEGGAQVDDSAPKFCAVAPSGG"
     gene            4544..5479
                     /locus_tag="VMZ53_09320"
     CDS             4544..5479
                     /locus_tag="VMZ53_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS28699.1"
                     /translation="MRLGSFLLRRLGSGALAILGVSVLVFLFLHLVPGDPVDHLLGGE
                     ATPAQRKQLEQCLGLDQSMPKQFVHFLGNIADGSLGHQCPDPKNKPTVASRILEVFPH
                     TIALAIAGMLVAILLALPLGVIAAVRRGTWIDTFATVTSLSVIGVPMMLLAPILLLVF
                     FIWLGWLPGPTETGPASLILPAVAVGLHLMAMLARMTRSSMIEVLGEDYVRTARAKGL
                     PEKRVLIHHALRNALLPVITVAGLQFGSLLTGAIVIEKVFARPGLGLTLLDAIGERNY
                     PVVQGTVLVIAAVYVVVNTLVDLAYGLADPRIRRA"
     gene            5476..6306
                     /locus_tag="VMZ53_09325"
     CDS             5476..6306
                     /locus_tag="VMZ53_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS28700.1"
                     /translation="MKAGIRISPSGRVGMVMVGVFIILGVIGPWIAPFDPTVPHLANR
                     FEGMSAVHWLGTDATGVDALSQLLHGARVALEISVVVVAISATVGVAIGTIAGWFRGA
                     VDEFLMALVNILMAFPGILLNIAVVATVAKPGIGMMITALCINGWVGYARVARGEVLA
                     LRERDFVMAAVALGASHRRVMLRHLVPNILGPAFVQMSFGFGSVILVEATLSFLGLGP
                     QVSYTWGAMLDQGTTFLWRAGFTHYALIPGIAITWVVLGANLLADGLRDRFDPRQRGR
                     "
     gene            6310..6624
                     /locus_tag="VMZ53_09330"
     CDS             6310..6624
                     /locus_tag="VMZ53_09330"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HUS28701.1"
                     /translation="MALRVRVCKLTEVVDDQLRAFPVSGVTWPVIVTRTDGELYACAG
                     VCPHEDVSLANGDLHGTVLTCPGHSYEFDLKTGVCIHDRTLHLRRYPITLLDDDVWVD
                     LM"
     gene            complement(6625..>6907)
                     /locus_tag="VMZ53_09335"
     CDS             complement(6625..>6907)
                     /locus_tag="VMZ53_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28702.1"
                     /translation="RSVRIVDVMAIPGEWHRCLSAIASHATTATDAHFVDIKLMALDG
                     RRRHMWRAGFTERDSKPFLVVIPTTGDRRFLDPMRWFYGGADSDLDFLD"
BASE COUNT         1119 a         2165 c         2351 g         1272 t
ORIGIN      
        1 aagtcgccgg cgagaacgag gtcgtgattc gtgcgcgcca tcgggagcgc gagcgtcgag
       61 gtccacgcac cgacggttcc gtcggcggcg agtggcgcgg catacacctg cgtcgtgccc
      121 ggagcgccga acacttgcgc gcggcctccg acgacgaaca gccactcgtc gacgacagtc
      181 gcttcgccaa agccgacagg ctgcggcagt gatgtcgtcg caatcgcagc accgcccgcg
      241 agcggtgaca gcgacacgtc ggggagcact ccgaccgtcg tgtcgtgata gccgccgacg
      301 gtatagataa agtgatcgga gaacgcgtac tcggcctgcg cgcggaagcc gatctgcaga
      361 tcgtgcgtgc tccacgtcag cgacgcgaca ggggaggtca gagcgaccgt ctgcccgctg
      421 tcattcgcgg gcactttcgt cttcatcacg attagctcgc cgttccgcac ggcggcttcg
      481 gtcgagatcg cgatatcggg caacgtgccc atcggcgata gtgcgccgag cattccgctg
      541 tcgtcgagcg tcgcggtcca caccttgcgg ctgtcttctt cgcggtcgta gatgccgtcc
      601 acgatgaaca gcgagtcggc atcgctggtc gcattgcatt ctgtgaccgg gctcggtgtg
      661 cgaccagcga gctgccagtt cccgagtgtc ccgtccggcg agagcttcgc cgcgtggatc
      721 tcgtcggtct tcacgaagcc gtcgctcgcc atgtagttgc cgccgatgac gaggacccaa
      781 tcgccgatcg cggcactgca gtgattcgcg cgaggcgtcg gcatcgcggc ggcgacgtgc
      841 cattcgccga gctcgcccgt tgcgacagtc gacgaagacg acgtacacgc aagaagcggc
      901 agaactagag cgacggcgat gcgcatacgg cgcaccttgc ggcgctttca cgcgtcatca
      961 caagcgtcgt gttgttgcac gtcggtgacg cgacctaggc cagcgaatta gttgttctga
     1021 agtgcgaggc tgatgcgaaa gctcgcgtcg ttctggttcg cgggcggcga gaaacgccag
     1081 tgcgcgacga tgtgcgagag gcactcgtcg aacttgcggc cacccgtgat gtcgctgagc
     1141 gcgtggccgt cggcgtcgac ttggaatgcg agcacgacct tgccggacag tgaaggatcg
     1201 gccgcgaggc tcttgcgata gcaacgctgc aggccactca tgtagatcgt gttgatcttc
     1261 gtcagcacct catcgacggt gaggcgcggc gtcgtgacga gggcgacgcg cgatggggaa
     1321 cgttcgacct tcgtacgctt gatcgtcgca gcgggccgga catcaccggt gtcgcgcggg
     1381 gtcgcgaact cggcgtgagc gatcgcgggg agcaggacgg cgacgacgag gatggcggtg
     1441 cgaagcatgg tgacggctat tgctcgcggc gggccaggta atgcgcgcca caagaccgcg
     1501 aaaggatgat caccgcagga gggtatgtac ggaccctggg gtagcttttt accccagtaa
     1561 acgatgtgcg attccggtga cttaaccgac cgctacctgg cgctagatct cgagctcgtc
     1621 gccttcgcga gcggggacaa ctccgatgcc gtgcggcgcg agcagctctt tgcagtgggc
     1681 gacgatctgg tcgacgtcgt cgtcgctgta gtcctggtcg tagtggaacg tgacaagcgt
     1741 cttcaccttg gcggcgatcg cggcggcgca cgcatcctcg taggacgaga agccgcgatt
     1801 gcggcgcgtc acctgatcgt cgggacgata tgtcgagtcg tgcagcagga catcggcgcc
     1861 gtggtagtgc gcgattgcat cggcgggcgg gccggattcg gggtagccga cgtcgctcgc
     1921 gtacacgaac gtgcgattgc cttcttggat ccggaaggag agcgcggtgg ttgcgccgtg
     1981 tgggtgctcg cgcgcgacga cgcgtaggcc ctcggcctcg aatggttggc cgcacgtgat
     2041 cgcgcagacg tcgaacgtcg ccgcgaggtt cttcagcgtt tggagcggcg agaagttcgg
     2101 gtccatctgg ccttcgagga tttcctcgag gacttcgggc gagccgccgg ggccccacac
     2161 cgtgaactga ttgcctggga tgaagaccgg cgggaagaag ccgaggcctt ggatgtgatc
     2221 ccagtgcgtg tgcgagagca ggatcgcggc gtggcctttg cctttgccga acgcttggcc
     2281 catcagcgtg ttgccgaggt gcatgaggcc ggtgcccatg tcgatgatga cgaggtcgcc
     2341 tttggccgtc gtgatcgaga cgcacgacgt gttgccgccg tagcgattcg tctcgggacc
     2401 tggtgcggga atcgagccgc ggacacccca gaagcggatc ttcattcgtc ccccaacggg
     2461 atctctagac cttcgtacgc gctcacgaga tcgaacttct cggctgcggt gacttcccgg
     2521 tactgcgcga ggatcgcgtc gttctcgtcg tcagagcgca gcggcgcgtg gtggaagagg
     2581 cagagcttct tcgccttggc ttcgcgcgcg atctcgatcg catcgtcggg acgcgagtgg
     2641 ccccagtgcg ggcggacctt gtactcgtcg ggggtgaact gcgtgtcgta gatgagaagg
     2701 tcggcgttct tgcagagcgc gacgacaccc gcgcgcatcg acttgagctc gtcttggatc
     2761 ggtggtggca gcgtgccgaa gctcgggcgc tcgatgaagt cgcggccaag gagcatgtcg
     2821 gtgaacggcg cggtgtcgct gcagtagacg accgatgcac cgtcgacgtc gacgcgatac
     2881 gcgatcgcga cccacggatg gttgagacgc gcgcaggtga ctttcgctgt gcccagctcg
     2941 aacgtcgcgc cttcgacgag ctcgtgaaac ttcatctcgg cgtgcatcgc ggagagcggg
     3001 acggggaagt acggcgcatt gtgctgctgg ctgaagacgg cctcgaggtg gaggtcgcgc
     3061 tggcgagcga agatgtgaat cttgttgccg gcgcggtaga gggggctgaa gaacggcagg
     3121 ccctggacgt gatcccagtg ggtatggctg atgaggacga acgcgtcgcc tctgccgtcg
     3181 ccgaacgagt cctccatgag cgatttgccc aggcggcgaa ttccggtgcc ggcgtcgatg
     3241 atgatcggcg agccgttttt cggccgcact tccacgcagg aggtgttgcc tccatagcga
     3301 ttcgtcgccc gcccggggac ggggatggag ccacggactc cccagaacct gaccttcatc
     3361 gggcgccaag acgaacctaa cgccgttatt gggcttgtcg ctagaagagc ttgcgtgaat
     3421 ctagaaggat cgtcaccggg ccgtcattga gcagctcgac cttcatatcg gccgcaaatt
     3481 cgccggcttc gaccttagcg actcccgcag cgcggatcgc gtcgagcgac tgctcgtaga
     3541 gagcttttgc ggcgacgggc tcgagcgcgc cggtgaacgc gggccgtcgg ccttggcgtg
     3601 catcgccgta gagcgtgaat tgcgagacga cgagcacgcc gccgacgatg tcgaggacgg
     3661 agcggttcat tttgccagcg tcgtcgggaa agatgcggag gttgacgatc ttgtcgacga
     3721 cgtactggag gtcggctgcg ccgtcaccgg cccctgcgcc gaggaagacg agcaggcctc
     3781 ggtcgatcga tcccgtgatc tgcgtgtcga cggtcacgga agctcgcgaa actcgctgga
     3841 cgacggccct catggcgcca ctttacaggc aaccggcagc ggcttgccgg gggtcacatc
     3901 ggcggctagg atgcgcccgc tcatgaggta cctggtgctc gcgatcgcgg tggcgacttt
     3961 gtcggctggc tgcggcaaag agatcggcga ctcgtgcgtc gtgagctctg actgcagccc
     4021 caacggcgat cgccagtgcg acgtctcgca aaaagagggc tactgcaccg tcatgggctg
     4081 cgatttctcg acgtgccccg atgaggccgt ctgcgtgcgc ttcttcaccg gcgggtttgg
     4141 caatcgcacg tgtgagtcct caccggacga gtgctcgctc gacgagctgt gcgacctcaa
     4201 caagcgctgc gtgccgcgca gctccgaagt tcgctactgc atgaagacgt gcgattcgaa
     4261 cgacgattgc cgcgacggct acgagtgccg cgacctcgca aagatgaagt cgcacggcgg
     4321 cgagcccgtc ctcgaaggcg gcgcccaagt cgacgacagc gcgccgaagt tctgcgcggt
     4381 cgctccaagc ggcggctagg ccgcaagcgg cacaagccgg gtcccgaagt gctgcgcgct
     4441 tcacccccct aggccgcaag cggcacaagg cgggtcccga agcgctgcgc gcttcacccc
     4501 cctaggaagt cgcgcgatag ccctggcgtc gtagtatcga cgcgtgaggc tcggttcgtt
     4561 cctgctgcgt cgcctcggct cgggcgcgct cgcgatcctc ggcgtgtccg tgctggtgtt
     4621 cctgttcctg cacctcgtgc cgggcgatcc cgtcgatcac ctgctcggcg gtgaggcgac
     4681 gccggcacag cgcaagcagc tcgagcaatg cctcggtctc gatcaatcga tgccgaagca
     4741 gttcgtccac ttcctcggca acatcgccga cggctcgctc ggccatcagt gccccgatcc
     4801 gaagaacaag ccgacggtcg cgtcgcgcat cctcgaggtg ttcccgcaca cgatcgcgct
     4861 cgcgatcgca ggcatgctcg tggcgatctt gctcgcgtta ccgctcggtg tgatcgcggc
     4921 ggtcaggcga ggcacgtgga tcgacacgtt cgcgacggtg acatcgctct cggtgatcgg
     4981 ggtgccgatg atgctgctcg cgccgatctt gctgctcgtc ttcttcatct ggctcggctg
     5041 gctaccagga ccgacagaga cgggccccgc atcgctgatc ttgcctgcgg tcgcggtcgg
     5101 cctccatctc atggcgatgc tcgcgcgcat gacgcgctcg tcgatgatcg aagtcctcgg
     5161 cgaggactac gtgcgcaccg cacgcgcgaa gggcttgccg gagaaacgcg tgctcattca
     5221 tcacgcgctc cgcaacgcgc tgctacctgt gatcaccgtg gcgggtctgc aattcggctc
     5281 gctgctcacg ggcgcgatcg tgatcgagaa agtcttcgcg cggccgggcc tcggcctcac
     5341 gctcctcgac gcgatcggcg agcgcaatta cccggtcgtg caaggcacgg tgctcgtgat
     5401 cgcggcggtg tacgtcgtcg tgaacacgct cgtcgacctc gcgtatggcc tcgctgaccc
     5461 gaggattcgc cgcgcatgaa ggccgggatt cgcatctcgc cgtcagggcg cgtcggcatg
     5521 gtgatggtcg gcgtgttcat catcctcggc gtgatcggcc catggatcgc gccgttcgat
     5581 ccgaccgtcc cgcacctcgc aaaccgattc gagggaatgt cggcggtgca ctggctcggc
     5641 accgacgcaa cgggcgtcga cgcactctcg cagctcctcc acggcgcgcg cgtcgcactc
     5701 gagatcagcg tcgtcgtcgt cgcgatctcg gcgaccgtcg gtgttgcgat cggcacgatc
     5761 gccggctggt ttcgcggcgc cgtcgacgaa ttcctgatgg cgctcgtgaa catcctgatg
     5821 gcgttccccg gcatcttgct caacatcgcg gtggtcgcga cggtcgcgaa accaggcatc
     5881 gggatgatga tcaccgccct atgcatcaac ggctgggttg gctacgcgcg cgtcgctcgc
     5941 ggtgaagtgc tcgcgcttcg cgaacgcgac ttcgtgatgg ccgctgtcgc gctgggcgcc
     6001 tcgcatcgcc gcgtcatgct gcgtcacctc gtgccgaaca tcctcggccc ggcgttcgtg
     6061 cagatgtcat tcggcttcgg cagcgtgatc ctcgtcgagg cgacgctgtc gttcctcggc
     6121 cttggcccgc aagtcagcta cacctgggga gcgatgctcg atcagggcac gacattcctc
     6181 tggcgcgcgg gcttcacgca ctacgcgttg attcccggca tcgcgatcac gtgggtcgtg
     6241 ctcggcgcca atctgctcgc cgacggcctg cgcgatcgct tcgatccacg ccagcgaggt
     6301 cgctgagcca tggcgctgcg cgtgcgcgtc tgcaagctca ccgaggtcgt cgacgaccag
     6361 ctgcgcgcgt ttcccgtgag cggcgtgacc tggccggtca tcgtcacgcg cacggacggc
     6421 gagctctacg cgtgtgctgg cgtctgcccg cacgaagacg tgtccctcgc gaatggcgat
     6481 ctgcacggca ccgtcctgac gtgcccgggc cactcgtacg aattcgatct gaagaccggc
     6541 gtatgcatcc atgatcgaac gctgcacctt cgccgctatc ccatcacgct cctcgatgac
     6601 gacgtctggg tagacttgat gtagctagtc gaggaagtcg aggtcggagt ccgcgccgcc
     6661 gtagaaccag cgcatcggat cgaggaagcg acggtcgccg gttgtgggaa tcacgacgag
     6721 gaacggcttg ctgtcgcgct cggtgaagcc tgcgcgccac atgtgacgac ggcggccgtc
     6781 gagcgccatc agcttgatgt cgacgaagtg cgcgtcggtc gcggtcgtcg cgtgcgatgc
     6841 gatcgccgac aggcagcggt gccactcgcc gggaatcgcc atcacgtcga cgatgcgcac
     6901 gctgcgg
//
LOCUS       DATLBZ010000178         5031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6048518, whole genome shotgun sequence.
ACCESSION   DATLBZ010000178 DATLBZ010000000
VERSION     DATLBZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6048518"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VMZ53_09340"
     CDS             complement(<1..560)
                     /locus_tag="VMZ53_09340"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HUS28703.1"
                     /translation="MALKKGVGVKPAEGKLGILLPGMGAVATTFIAGVQAIRRGLGKP
                     IGSLTQLGHIRIGKRTDNTSPAIKDYVSLANLDDIVFGGWDIFPDNAYQAAVRAGVLD
                     TRLLDQLKPELEAIKPMTAVFEQAYVKKLNGPNLKKGTSKMDLANQVMADIKTFKETN
                     NCSRLVAVWCGSTEVYRMPGDVHSSLA"
     gene            complement(648..1295)
                     /locus_tag="VMZ53_09345"
     CDS             complement(648..1295)
                     /locus_tag="VMZ53_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28704.1"
                     /translation="MRTLITVDGIDGSGKSTFARRIADAFEAAGVRTQIVRVDDFRRP
                     IDWSDTEGKEADVYYDAYYDFELCERSLQAYFAGAAKVEIPAYDSRTERIDGTRELDF
                     DGVQVVLVEGVFPRRLAATGQALTIYLSVGEDEARRRIIARDLKKGRTREEIERRIDR
                     RYFPSQRRYHAEFLPLEKADVVVENDHAEAPRVVHRDLNGLHPELRAILDRVLGA"
     gene            complement(1296..2102)
                     /locus_tag="VMZ53_09350"
     CDS             complement(1296..2102)
                     /locus_tag="VMZ53_09350"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HUS28705.1"
                     /translation="MYDRELEVAERAARTAGDVIMRHYARGGIDVEAKADATPVTAAD
                     RDANAAIIEVLRAAFPDDYVLSEELPDDDARLTRSRVWIVDPLDGTRDFVQRTGEFAV
                     HVGLAIDGRAVAGAVHVPVAGATYVAQLGGGAWRITGSGKDRLRVSPTSALDEIRVGV
                     SRLNPSPTLAPFLAKTGLAQRTVGMGASVKFMALAEGRLDAVINASTSEHEWDTCAPE
                     VILREAAGTITDLDGADFRYNQRALSHPRGSLASNGVCHDLLLGLLRDHL"
     gene            complement(2122..3990)
                     /locus_tag="VMZ53_09355"
     CDS             complement(2122..3990)
                     /locus_tag="VMZ53_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HUS28706.1"
                     /translation="MSRAVVLATRPAFESGSELSAGFVLSVRQTLERLSALGVRDIAV
                     VDGRDAEQLRERLALHELPQISVTVIANQSWRNASGSALLLAKAWVDRPGSCLVVRGD
                     RPLDVESLRALVQLGSVEKGDATIVVAMTAEVEPGADVRVKLTREADSEHYAVTSLGE
                     DLVCADGVFTGHALVQRSIIPVLQSLPNPTLHSGLLQLARSGRVLAVTSVSWAWGARS
                     RIDVEDNVTALLQAKRHATYTLLNPGPVNTTATVKSALVHPDVCHRDSIFSELMVSMT
                     ERLRRIFRGTPHHTVVPITGSGTAAMEAALVSTVPPDRKVLIIDNGAFGHRLVEVATV
                     HEMNVLHLRYEWGEAVRVEDVERAFQEHSDIGVVAMIHHETSVGLLNPVREIGALCKK
                     HDALLVVDAVSSLGAEDIDVVRDNIDICYSSANKCLHAVSGASFMCVAPRVWPRIELL
                     SPRSYYLDLRRYRKYMDELAQTPFTPAVSVYFALDAACAEFLADGHAARFEMYRSRNR
                     KLRDGLTALGMTSFTNTGLESSSIVTCRIPEDIVFQDLYDAVKARGIILYGCKGVLAE
                     RYMQIANMGHLSDSSIEEFLMVLREEIKRLSTHTRVSVPATAVAREKSASERRRVG"
     gene            4156..4890
                     /locus_tag="VMZ53_09360"
     CDS             4156..4890
                     /locus_tag="VMZ53_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HUS28707.1"
                     /translation="MKAVILAAGVGSRLGDETNDKPKPLVKIHHRSLLFRQLDSLAKA
                     GIAPENVVVVGGYRINQIADALTSGGFHSTKLVLNERFEPPWGNFLSLLSAAPELRGH
                     PFLQVDGDLLVDDEILPALIAARGEALLATDPHAELDGDAMKVELDANGVLVSLDKVK
                     VDMKKVVGEFIGVTKLSAAAGEAVFAELDKFLGEGLTDQYYERAYERLSRSGAVPFGV
                     HLLAKSAVWREIDDAVDLADARAKFF"
BASE COUNT          886 a         1671 c         1592 g          882 t
ORIGIN      
        1 gcgagcgagc tgtggacgtc gcccggcatg cgatagacct cggtcgagcc gcaccacacc
       61 gcgacgagac gagagcagtt gttcgtctcc ttgaacgtct tgatgtcggc catcacctga
      121 ttggcgaggt ccatcttcga cgtgcccttc ttgaggttcg ggccgttgag cttcttgacg
      181 tatgcctgct cgaacacggc cgtcatcggc ttgatcgctt cgagctcggg cttgagctga
      241 tcgagcaggc gggtgtcgag cacgccggcg cgaaccgcgg cttgatacgc gttgtccggg
      301 aagatgtccc agccgccgaa cacgatgtca tcgaggttcg cgagcgagac gtagtccttg
      361 atggccggcg acgtgttgtc ggtccgcttg ccaatgcgga tgtgaccgag ctgcgtcagc
      421 gagccgatcg gcttgcccag cccacgccgg atcgcctgca caccggcgat gaacgtggtg
      481 gccaccgcgc ccataccggg gagcaggatg ccgagcttgc cttcagccgg cttgacgccg
      541 acgcctttct tgagagccat gtcgaatctc cgcgctttgg aaacgcggaa ggtgccgcac
      601 cgcgacgttt cgcgcaagcc cttgatcggg cccggccccg agggtgatca agctcccaaa
      661 acgcggtcga ggatcgcccg gagctcgggg tgtaacccgt tcaggtcacg atgaacgacc
      721 cgcggcgcct cggcgtgatc gttctcgacc acgacgtccg ccttctcgag cggcaaaaac
      781 tccgcgtggt agcgacgttg cgacgggaag tagcgcctat caatgcgccg ctcgatctcc
      841 tcgcgcgtcc tgcccttctt tagatcacgc gcgatgatcc ggcggcgcgc ttcgtcttcg
      901 cccaccgaga ggtagatcgt cagcgcctgc ccggtcgccg caagacgccg cgggaatacg
      961 ccctcgacga gcaccacctg cacgccatcg aagtcgagct cgcgcgtacc gtcgatgcgc
     1021 tcggtgcggc tgtcgtacgc cggaatctcg accttggcgg cgccggcgaa gtaggcctgc
     1081 agggaacgct cgcagagctc gaagtcgtaa tacgcgtcgt agtagacgtc ggcctctttg
     1141 ccctcggtgt cgctccaatc gatcggtcgt cggaaatcgt cgacgcgcac gatttgcgtc
     1201 cggacgcccg cagcctcgaa tgcgtcggcg atgcggcgag cgaacgtcga cttcccgctg
     1261 ccgtcgattc catcgactgt aatgagcgtg cgcatctaga ggtggtcgcg gagcaagccg
     1321 aggagaaggt cgtggcaaac gccgttgctg gcgaggctgc cccggggatg tgacagggcg
     1381 cgctggttgt atcggaagtc agccccgtcg agatcggtaa ttgtgcccgc cgcctcgcgg
     1441 aggatcactt cgggggcgca cgtgtcccat tcatgctcgc tcgtgctcgc gttgatgacc
     1501 gcgtcgaggc ggccctcggc gagcgccatg aacttcacgg atgcgcccat tccgacggtc
     1561 cgctgcgcca gtcccgtctt ggcgagaaat ggcgcgagcg tcgggctggg gttgagccgg
     1621 ctcactccga cgcgaatctc gtcgagcgcg ctggtcggag aaactcgcag tcggtctttg
     1681 ccgcttcctg tgatccgcca tgcgccgccg ccgagctgcg cgacgtaggt cgctccggcc
     1741 accggaacat gcaccgcacc ggcgaccgcg cgcccgtcga tcgcgagacc gacgtgcacc
     1801 gcgaactcgc ccgtgcgctg cacgaagtcg cgcgtgccgt cgagtggatc gacgatccag
     1861 acgcgtgagc gcgtgagccg cgcgtcgtcg tcgggcagct cttcgctgag gacgtagtcg
     1921 tccgggaacg ccgcccgcag cacctcgatg atcgcagcat tcgcatcgcg atcggccgcc
     1981 gtgacaggcg tggcgtccgc cttcgcttcg acgtcgatgc cgccgcgcgc gtagtggcgc
     2041 atgatgacgt cgcccgctgt acgcgctgcc cgttcggcaa cctccagctc gcggtcgtac
     2101 acgctgcgct ccagtgacta gctagccgac gcgacgacgt tcgctcgcgc tcttctcgcg
     2161 cgcgaccgcg gtcgctggaa cggagacgcg ggtgtgcgtg ctgaggcgct tgatctcttc
     2221 gcgcagcacc atgaggaact cttcgatgct cgagtccgac agatggccca tgttggcgat
     2281 ctgcatgtac cgctcggcga ggacgccctt gcagccgtac aggatgatcc cgcgcgcctt
     2341 cacggcgtcg tagagatcct ggaacacgat gtcctcggga atgcggcacg tgacgatcga
     2401 gctcgactcg agccccgtgt tcgtgaacga cgtcatgccg agcgcggtca agccatcacg
     2461 cagcttgcga ttgcgcgagc gatacatctc gaagcgcgcg gcgtgaccat ccgcgaggaa
     2521 ctccgcgcat gcagcgtcga gcgcgaagta caccgacacg gcgggcgtga acggcgtctg
     2581 cgcgagctcg tccatgtact tccggtagcg gcgcaggtcc aagtagtacg agcgcggcga
     2641 gagcagctcg atgcgaggcc acacgcgcgg cgcgacgcac atgaacgatg caccgctcac
     2701 ggcatgcagg cacttgttcg cgctcgagta gcagatgtcg atgttgtcgc gaacgacgtc
     2761 gatgtcttcg gcgccgagcg acgagacggc atcgacgacg agcagtgcat cgtgcttctt
     2821 gcacagcgcg ccgatctcgc gaactggatt gagcaggccc accgacgtct cgtgatgaat
     2881 catcgcgacg acgccgatat ccgagtgctc ctggaacgcg cgctcgacgt cttcgacgcg
     2941 aacggcctcg ccccactcgt agcgcaggtg caagacgttc atctcgtgaa ccgtcgcgac
     3001 ctcgacgaga cgatgaccga aggcaccgtt gtcgatgatg agcactttgc gatccggcgg
     3061 aacggtcgac acgagtgccg cttccatcgc cgcggttcca ctgcccgtaa tcggcaccac
     3121 ggtgtgatgc ggcgtgccgc ggaagatgcg acgcaggcgc tcggtcatcg agaccatcag
     3181 ctcgctgaag atcgaatcgc ggtggcaaac atcggggtgc acgagcgcgc tcttcacggt
     3241 agccgtcgtg ttaacgggcc ccgggttcaa gagcgtgtac gtcgcgtgac gttttgcctg
     3301 cagcagcgcc gtgacgttgt cttcgacgtc gatgcggctg cgtgcgcccc acgcccacga
     3361 cacgctcgtc accgcgagaa cgcggccaga ccgtgcaagc tgcagcaatc ccgaatgcag
     3421 cgtgggattg ggcaggctct ggagaacagg aatgatgctg cgctgcacga gcgcgtggcc
     3481 cgtgaagacg ccgtctgcac agacgagatc ctcgccgagc gacgtgactg cgtagtgctc
     3541 gctgtcggcc tcacgcgtga gcttcacgcg aacgtcagcg ccgggctcga cctcagcggt
     3601 catggccacg acgatcgtcg catcgccctt ctctaccgag ccgagctgca cgagcgcgcg
     3661 cagcgactcg acatcgaggg gccgatcgcc acgaacgacg agacagctgc ccggccgatc
     3721 gacccacgcc ttggccaaca gcagcgcgga cccgctagca ttccgccagc tctggttcgc
     3781 gatcaccgtg accgaaatct gaggaagctc gtgaagcgcc aaccgctcgc gaagctgctc
     3841 tgcgtcccgc ccatccacca ccgcgatatc gcggacaccc aacgccgaaa gcctctcgag
     3901 cgtctgccgc accgacaaga cgaacccagc tgaaagctcg ctgcccgact cgaaggcggg
     3961 acgcgttgcg aggactactg cgcgcgacac gggcatagat gcttcaatcc caccgggtaa
     4021 cgaggctaac gagcaaaaca gatggccgaa tcaaccactt ggcagtcctc acgtcaacac
     4081 cgtttcgtcg ccgtgacaac tgtttgacgt gttctgcgtt gactcgatct ccccatcacg
     4141 tactgtgccg cggttatgaa agcggtgatc ttggcggccg gcgttggctc gcgcctcgga
     4201 gacgagacga atgacaagcc aaagcccctg gtgaaaattc accaccggtc gctacttttt
     4261 cgacagctcg acagcctggc aaaagcaggc atcgctcccg agaacgtggt cgtcgttggc
     4321 ggctatcgca tcaaccagat cgccgatgcg ctcacgagcg gtggtttcca ctccacgaag
     4381 ctcgttctca acgagcgctt cgaaccgccg tggggcaact tcctctcgct cctctccgcc
     4441 gcgcccgagc tgcgtggaca cccattcctc caagtcgacg gcgacctcct agtcgatgac
     4501 gagatcctcc cggctctcat cgccgcccga ggcgaagcgc tgctcgcaac cgatccacac
     4561 gcggagctcg atggcgacgc gatgaaggtc gagctcgatg ccaatggcgt cctcgtctcg
     4621 ctcgacaaag tgaaagtcga catgaagaag gtcgtcggcg agttcatcgg cgtcacgaag
     4681 ctctccgccg cggcaggcga agccgtgttc gccgagctcg acaagttcct cggcgaaggc
     4741 ctcaccgacc agtactacga gcgcgcgtac gagcgcctca gccgctccgg cgccgtcccg
     4801 ttcggcgtgc atctgctcgc gaagtccgcg gtgtggcgcg agatcgacga cgcggtcgac
     4861 ctcgccgacg cgcgcgccaa atttttctag agctgcgatt cgcagccgtg tcggctgcaa
     4921 tttgcagcat ggctctgctc tatcctcgtg caatccgtcg cgaagacgct gggcgtgcgc
     4981 ttgcacgatg cgcggcggat ggcacacgag gctctgcacc attttctacg c
//
LOCUS       DATLBZ010000179         4784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3899038, whole genome shotgun sequence.
ACCESSION   DATLBZ010000179 DATLBZ010000000
VERSION     DATLBZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3899038"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..519)
                     /locus_tag="VMZ53_09365"
     CDS             complement(10..519)
                     /locus_tag="VMZ53_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28708.1"
                     /translation="MARSYRFKPRYKALAISAMGVGGTLGVVSAIALGAAALPLATGA
                     LGIALGGGYLLSPSWKLEVVVDDDALIVRSPKAEKFRIAWLEVKRVVASPSTKTCFVD
                     VKDPARNFLVPGDGAPAPYDIEDREELYAAILAKVPREKIEEVETIEAAKKASLAKPA
                     AKADAKPDA"
     gene            complement(612..1037)
                     /locus_tag="VMZ53_09370"
     CDS             complement(612..1037)
                     /locus_tag="VMZ53_09370"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS28709.1"
                     /translation="MTDSRRAPRLVVNAPAVVESIGQTSIQLHPNLAKVFRRVEADRS
                     SLGTKFPAVVRDLSTNGAFISGAPLPLLSRVAIKFDVKGVGNIDAIGWVLWQRMEDCE
                     VPSAVGSATLPKGFGVLFEAIPMETRAAIAALVTKMGGA"
     gene            complement(1048..3129)
                     /gene="metG"
                     /locus_tag="VMZ53_09375"
     CDS             complement(1048..3129)
                     /gene="metG"
                     /locus_tag="VMZ53_09375"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HUS28710.1"
                     /translation="MTFYITTPIYYVNDVPHLGHAYTTIVADALARFHRMRGEDTRFL
                     TGTDEHGQKVEETATKKGLTPQQLVDQVAPRFDETWKTLGIEGYRFIRTTDDKHKRVV
                     ATLWRAIKKKNPDDLYLASYQGWYCVGCEAFYTESQLVKDGDQWLCSVHKKPVNWIDK
                     ERSWFFKLSRYAEPLLEHIAKNPDFIRPEAYRNEIVAFLKSGLKDLSVSRTSFAWGIP
                     VPEDDTEGMKHVIYVWMDALTNYLSDLCPESGEIGGPDVAKYWSEAIHVIGKDILRFH
                     TVYWPAFLMSAGLPLPKSIFAHGWWTVRGEKISKSMPATRIDPVKLADALAVGPLGRP
                     IGIDAMRYYLLREVPLGFDGDFTFESLFGRFNAELANDLGNLVNRSLTLMAKSNLHPA
                     YDMAQSEVDKHASFAKVANDAIVAATAEYEAMAPSKALEAIWKLVREANRYVDLNQPW
                     ALAKDPSKAIELAHVFHNLANAIGVIGGLVSPILPTTGRLLRQWVGLTGADEVAWPTS
                     SDMLKVVGNVSKDPKPMYPRLDEAAQTKILTQIVPGDIAGAAASAPAPATPATQKPTA
                     KGGDAKAGEPAKAADGAPGTITYDDFAKLDLRVGKVLSAVAVPKKDKLLHLQVDLGEG
                     KPRSIIAGLALSYKPEALVGKNVIVVANLAPRKMAGLVSEGMILASGEGDSELGVSAL
                     DRDAAPGTRVR"
     gene            complement(3126..4292)
                     /gene="ricT"
                     /locus_tag="VMZ53_09380"
     CDS             complement(3126..4292)
                     /gene="ricT"
                     /locus_tag="VMZ53_09380"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HUS28711.1"
                     /translation="MSAASSGSMSAGASMPRIGGASRGSQPHVGDDDEEDGRSSEPST
                     TAWGTDDGAPAPDVKLAADLPSEPPADDPDPTTYHLVDSPGATLGDDVASVVGVRFVP
                     AGRIQWCDAGDSDFVPGERVLVDSDRGQRLAWIATVPTRRGVRDRNLRRVIRRANEID
                     LRGELAGEHDRKELLRAAKDAAERLRLPLKVFRVEKSGSKLNVYYTSDDRLDLRDFVR
                     DVGQATGLRLELRQLGVRDEAKMVGGIGSCGLTLCCTTWLPDFVPVSIKMAKDQGLVL
                     SPTKVSGQCGRLKCCLVYEQAGYAEMRKGLPKLGKRVIAPRGEGRVVEVDVLRQRIRV
                     SYGPGDTEVLPATEVKPMFPSGNQPRGGPGGHGAQDADDDGHDPSSDEPDLPTS"
     gene            complement(4289..>4784)
                     /locus_tag="VMZ53_09385"
     CDS             complement(4289..>4784)
                     /locus_tag="VMZ53_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28712.1"
                     /translation="DTAAAIGRVADTAAAIGRLADTAPAIGRLADTAVAIVRVPDTAV
                     AIGRVPDTAAAIGRVPDTAAAIGRLADATDAERAVVAMIGLAAVVGATIVAGAAGVAA
                     MSAADAAASIAISAGARIARTRGSRPICARSPTTFCAAIRTVRVARVARLAVRSATQL
                     RRGR"
BASE COUNT          735 a         1695 c         1531 g          823 t
ORIGIN      
        1 gcgttgaggc tacgcgtcgg gcttcgcgtc ggccttggcc gctggcttcg cgagcgaggc
       61 cttcttcgcg gcctcgatcg tctcgacttc ttcgatcttc tcgcgcggga ccttcgcgag
      121 gatcgcggcg tacagctctt cgcgatcctc gatgtcgtac ggcgcggggg cgccatcgcc
      181 cggcacgagg aagttccgcg cggggtcttt gacgtcgaca aagcacgtct tcgtcgaggg
      241 cgacgcgacg acgcgcttca cctcgagcca cgcgatgcga aacttctcgg ccttcggcga
      301 acggacgatc agcgcgtcgt catcgacgac aacctcgagc ttccacgacg gcgacagcaa
      361 atagccaccg ccgagcgcga tgcccagcgc ccccgtcgcg agcggcagcg cggcagcgcc
      421 gagcgcgatc gccgagacga cgccgagcgt tccgcccacg cccatcgccg aaatcgcgag
      481 cgccttgtag cgaggcttga agcgatagct acgcgccacg aaacctcacc gaggcgcgcg
      541 atgcgtgcgg ctgcgacgag cgcgatgcgt gcggctgcga tgagctgcgc atgacgcggc
      601 ccagaggcgc gttacgcgcc gcccattttg gtgacgagcg cagcgatggc ggcgcgcgtc
      661 tccatcggga ttgcttcgaa gagcacgccg aagcctttgg gcagcgttgc gctgccgacg
      721 gccgacggca cctcgcagtc ttccatccgc tgccagagca cccagccgat cgcgtcgatg
      781 ttgccgacgc ctttgacgtc gaacttgatc gccacgcgcg acagcagcgg cagcggagca
      841 cccgagatga acgcgccgtt ggtcgagagg tcgcgcacga cggcggggaa cttcgttccc
      901 agactgcttc ggtcggcctc gacgcggcgg aacaccttcg cgaggttcgg gtggagctga
      961 atcgacgtct ggccgatcga ttcgacgacg gcaggcgcgt tgactacgag gcgtggtgca
     1021 cggcgagagt ccgtcatgct gcaggcttta tcgtactcgc gttcccggtg cagcgtcgcg
     1081 gtcgagggcg gaaactccga gctcgctgtc gccttcgccg gacgcgagga tcatgccttc
     1141 ggagacgagg ccggccatct ttcgcggcgc caggttggcg acgacaatga cgttcttgcc
     1201 gacgagcgct tcgggtttgt aggacagcgc gaggcccgcg atgatcgagc gcggcttgcc
     1261 ctcgccgaga tcgacctgca ggtggagcag cttgtccttc ttggggacgg cgaccgcgga
     1321 caggactttg ccgacgcgca ggtcgagctt cgcgaagtcg tcgtacgtga tcgtgcctgg
     1381 cgcgccatcg gcggccttcg ccggctcgcc ggccttcgca tcgccgcctt tcgctgttgg
     1441 cttctgcgta gcgggcgtcg caggcgcagg cgcagatgcg gcagcgccag cgatgtcgcc
     1501 tggcacgatc tgcgtcagga tcttcgtctg cgccgcttcg tcgaggcgcg ggtacatcgg
     1561 cttcggatcc ttcgagacgt tgccgacgac cttcagcatg tcgctcgacg tcggccatgc
     1621 gacttcgtcg gcgccggtga ggccgaccca ctgacggagc aggcgtcccg tcgtcggcag
     1681 gatgggcgac acgaggccgc cgatcacgcc gatcgcgttc gcgagattgt ggaacacgtg
     1741 cgcgagctcg atggctttgc tcggatcttt ggcgagcgcc cacggttgat ttagatcgac
     1801 gtagcggttc gcttcgcgca cgagcttcca gattgcctcg agcgccttgc tcggagccat
     1861 cgcctcgtac tccgcggtcg ccgcgacgat cgcatcgttc gcgactttgg cgaacgaggc
     1921 gtgcttgtcg acttcggact gcgccatgtc gtacgccgga tgcaggttgc tcttcgccat
     1981 cagcgtgagg ctgcgattga cgaggttgcc gaggtcgttc gcgagctccg cgttgaagcg
     2041 cccaaatagc gactcgaacg tgaaatcacc gtcgaagccg agcggtactt cgcgaagcag
     2101 gtagtacctc atcgcgtcga tgccgatcgg ccggccgagc gggcccaccg cgagcgcatc
     2161 ggcgagcttc accggatcga tgcgcgtcgc cggcatcgac ttgctgatct tctcgccgcg
     2221 gaccgtccac cagccgtgcg cgaagatgct cttcggcagc ggcagtcctg cagacatgag
     2281 gaacgccggc cagtacacgg tgtgaaagcg aaggatgtcc ttgccgatga cgtgaatcgc
     2341 ctcggaccag tacttcgcga catcgggccc gccgatctcg ccactctccg gacacaggtc
     2401 cgagaggtag ttcgtgagcg cgtccatcca cacgtagatg acgtgcttca tgccctcggt
     2461 atcgtcctcg gggacgggga tgccccacgc gaagctcgtc cgggaaacgg acaggtcctt
     2521 gaggccgctc ttcaagaacg cgacgatttc gttgcgatac gcctcgggcc gaatgaagtc
     2581 ggggttcttc gcgatgtgct cgagcagcgg ctcggcgtac ctcgagagct tgaagaacca
     2641 gctgcgctct ttgtcgatcc agttgaccgg cttcttgtgt acggaacaga gccactggtc
     2701 gccgtctttg acgagctggc tctccgtata gaaggcctcg cagccgacgc agtaccagcc
     2761 ctgatacgac gcgaggtaca ggtcgtcggg gttcttcttc ttgatcgcac gccacagcgt
     2821 cgcgacgacg cgcttgtgct tgtcatccgt cgtgcgaatg aagcggtagc cctcgatgcc
     2881 gagcgtcttc cacgtctcgt cgaagcgcgg cgcgacttga tcgacgagct gctgcggcgt
     2941 tagccccttc ttcgtcgcgg tctcttcgac cttctggccg tgctcgtcgg tgcccgtcag
     3001 aaaccgcgtg tcctcgccgc gcatgcggtg aaagcgcgcg agcgcatcgg ccacgatcgt
     3061 cgtgtacgcg tgaccgaggt gaggaacgtc gttgacgtag tagatcggcg tcgtgatgta
     3121 gaacgtcatg aggtaggcaa gtccggctcg tcgctgctgg ggtcgtgccc gtcgtcgtcg
     3181 gcatcctgcg cgccgtggcc accaggccca ccgcgcggct gattgccgga cgggaacatc
     3241 ggctttacct cggtcgcagg caagacctcg gtgtcgccag gtccataaga tacgcggatg
     3301 cgctggcgaa gcacgtcgac ttcgacgacg cgcccttcgc cgcgcggcgc gatcacacgc
     3361 ttgcccagct tcggcaggcc cttacgcatc tccgcgtacc cggcctgctc gtacaccaag
     3421 caacacttca ggcggccgca ctgcccacta accttcgtcg gcgacagcac caagccctgg
     3481 tccttcgcca tcttgatcga cacgggcacg aaatccggca accacgtcgt gcagcacagc
     3541 gtcaggccgc acgacccgat gccgccgacc atcttcgcct catcgcggac gccgagctgc
     3601 cgcagctcga ggcgcaggcc agtcgcttgg ccgacgtcgc gcacgaaatc gcgcaggtcc
     3661 agccgatcgt cgctcgtgta atagacgttg agcttgctgc cgctcttctc gacgcgaaac
     3721 actttgagcg gcagccgcaa tcgctccgcc gcatccttcg ccgcgcgcag cagctcctta
     3781 cgatcgtgct cgccggcgag ctcgccccgc aaatcgatct cgttcgcgcg ccggatgacc
     3841 cgccgcaaat tgcgatcgcg gacgccgcgc ctcgtcggca ccgtcgcgat ccacgcaagc
     3901 cgctgtccgc gatcactgtc gacgagcacg cgctcgcccg gcacaaagtc gctatcgccc
     3961 gcatcgcacc actgaatgcg gcccgccggg acaaacctga cgccgaccac actcgccacg
     4021 tcgtcaccga gcgtcgcgcc cggactatcg accaagtgat acgtcgtggg atccggatca
     4081 tcggccggcg gctcgctcgg caagtcggcc gccagcttca cgtcgggcgc aggcgcgcca
     4141 tcgtcggtgc cccacgccgt cgttgatggc tcgctcgagc gcccgtcttc ctcgtcgtcg
     4201 tcgcccacgt ggggctgcga gccgcggctc gcgccgccaa tgcgcggcat cgaggcaccc
     4261 gcgctcatcg atccggacga cgctgcgctc atcgaccccg acgcagctgc gtcgccgacc
     4321 gcaccgccaa ccgcgccacc cgcgccaccc gaaccgtacg gatcgccgcg cagaatgtcg
     4381 tcggactcct cgcgcagatc ggcctcgagc cccgggtcct ggcgattctc gcgcccgcgc
     4441 tgatcgcgat actcgccgcc gcgtccgccg cgctcatcgc cgccacgccc gccgcgcccg
     4501 ccacgatcgt cgcgcccacg accgccgcca gcccgatcat cgcgaccacc gcgcgctccg
     4561 cgtccgtcgc gtccgccagc cggccgatcg ccgccgccgt gtccggcacc cggccgatcg
     4621 ccgccgccgt gtccggcacc cggccgatcg ccaccgccgt gtccggcacc cggacgatcg
     4681 ctaccgccgt gtccgccagc cggccgatcg ccggcgccgt gtccgccagc cggccgatcg
     4741 ccgccgctgt gtccgccacc cggccgatcg ccgccgctgt gtcc
//
LOCUS       DATLBZ010000180         8934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6049384, whole genome shotgun sequence.
ACCESSION   DATLBZ010000180 DATLBZ010000000
VERSION     DATLBZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6049384"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VMZ53_09390"
     CDS             <1..788
                     /locus_tag="VMZ53_09390"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUS28713.1"
                     /translation="AELRGQLRELTVYFSDLAGFTSLAETRRPDELVTFLSEYFDDMS
                     KIIAAERGTVDKYIGDGIMAFWGAPLPLEQHAVHACIAALRCQERVRELSKSGVELVT
                     RIGLATGEVLVGNIGSTERLNYTVMGDIANLASRLEGLNKQYGTDLLISEKTYEQARS
                     KIVARPLDVVAVKGKKEGVRVFELLAIRGEDKAAEAIEDASTRALTAYLARDFATAIA
                     AWNEVLAKRPADKAATTMRDRAQAFATTPPPPEWTGVMIAKEK"
     gene            909..2468
                     /locus_tag="VMZ53_09395"
     CDS             909..2468
                     /locus_tag="VMZ53_09395"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HUS28714.1"
                     /translation="MKTSLVAISLALVACGSDAKTLPPTDPAELQTTLDALAAFGQKQ
                     PGTPAGEQAAAYIGDRFRALGLTDVHDEKFTLPAWSATKSFTLSIDGVQSTPGFDVFE
                     ASSSGTVNAVIVDVGTAMDAELEGKDLTGKIALVKRETSFHRSAQLRNVAAHGALAML
                     YLSVAPENLRQVGSVRFGWNAMEPIPAITIGADDGATIKAALAANKVVPAAIDVQATT
                     TPASGINVVGKIPGERPEMIVMGAHYDSWFTGSSDNGSGVAELLAVASRRMQRGKPRY
                     TLVFVAYDGEETGLYGGYDYYRKHKIVGGDSILAVLNFESPSALNPDIAGLAHSNQPK
                     LDEALQAAHLRQIYALYTGMEIVAQLFGGIIPTDIQGAYRGGTPTASTAVTNPYYHTV
                     KDTPDKVDLALLVESTDAFDAAIDNLGKLTPEDFAVADPQLWTAEVAVTGTTVDVTVK
                     TGSGAPGANAKVEAAVMHDDFMLDGEVNATTDASGHVTLTIPASTGAKRFLHVTAGPV
                     YPLAETIIALP"
     gene            complement(2550..2972)
                     /locus_tag="VMZ53_09400"
     CDS             complement(2550..2972)
                     /locus_tag="VMZ53_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28715.1"
                     /translation="MEREDYIHRLIRQFAEFLAKLAGLREKGEYRQALVATADAWEGM
                     LGHPRSIVDVVDTATLASLLGGPDKMRLGVKLLWEEGKTLTASGDPVHATICYRRAVE
                     LLLEARAIAPADEDSTMLFELGRVVQMNQLDPKYRTDD"
     gene            3059..4417
                     /locus_tag="VMZ53_09405"
     CDS             3059..4417
                     /locus_tag="VMZ53_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUS28716.1"
                     /translation="MGGRARSIIHVHHRGDRGVDGILRLIELASHDGPLEAMLTAMCD
                     EIASIAGVEIASVYVAEDERLVMRGNHGFDESAIGSVSLGVGEGITGLVAECMRPVSA
                     AHAATEAAYKHVPGLGEERFPVFAGIPLITGGAVVGVLVMQRRKKPFALDEVTLATAL
                     AAPITLAIERRKATAVRSARLTGVGHGGAVVLGRAGIVPTATAIGTAPFASLIAPHGP
                     ASPSDSSDDIRDGDNPIAAVIDRVLARIRDDLGRAMKKLGDIEDPVIGACLDRYALAL
                     VDGRLRERIIAAAQEPSGLRSVAKDYARAATRIASRRPPTTPPSSLSQDSRPPELDAE
                     TAERVAEIEELCALIGLTADARSSARPGASWIADRVGPVITVAAIARGAAAVVASDRV
                     TPTALAIAAAAKLPVVSGVAGLFGWARPGDLLAVDGESGTVLVHPAPTEIERLRRERS
                     PS"
     gene            4537..4896
                     /locus_tag="VMZ53_09410"
     CDS             4537..4896
                     /locus_tag="VMZ53_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28717.1"
                     /translation="MRFALYALVLVGCGDGVDRDIEGQITIDQGVYGLLISGCDTSDC
                     QDRPAAGEHVMVYSPGLNTVYATGMSDRSGVYQLDLEPGDYTLCTYACTPITVPASDR
                     VRYDWTSGPGGGHWEAD"
     gene            complement(4898..6424)
                     /locus_tag="VMZ53_09415"
     CDS             complement(4898..6424)
                     /locus_tag="VMZ53_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HUS28718.1"
                     /translation="MNVACLDLPALPLQLVWRTEPELRAHAVVVIDEDRPQGNVLWAC
                     ERARAAGVLPGQRYAHALSLHRALRARVVPPEKIDAAIVELRAALHALSPRVEPGDPG
                     VFWLDGEGLDRIFPDAPTPPGARGLKGQAWGMAIQRAIAALQLSGAVVVGFSRFATYA
                     IARATRHGVTVLRSDADERTAAAAVPLARLDIDPKLRDSLARLGVTTLGQMVRLPGGG
                     ILERFGRDAHRMYQLAAGERWDPLVPMAPPEAPDEYVVLDDDEHDVERLVFFLKAAID
                     RLLDRLAARGRALTALHVELTLREHVGQTEVRADCIKPAVPTLDARVLVRLVHLRLTG
                     MPPVAPVNAARVWADDVAATREQLAMFAQKPRRDLKAADEALARVRAELGDDAVVRAV
                     LREGHLPEASFGWERLQHVVHAKPKPVRVRPLVRRLYAKPQLLPPQARQVRDDGWLLS
                     GLEHGAVVRILGPYVISGGWWQHELHREYHFAELRRGDCLWVYYDRNRRRWFCQGAVE
                     "
     gene            6502..7278
                     /locus_tag="VMZ53_09420"
     CDS             6502..7278
                     /locus_tag="VMZ53_09420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS28719.1"
                     /translation="MKDKVIVITGASEGIGATTAELLAKRGARVVLAARRLDKLAAVR
                     ARIGDNALSVVTDVTNRADNENLRDKAIEKWGGIDVWINNAGRGISRAVAELTDEDID
                     DMVSINIKSVLYGVQAVLPHFKENKRGQIISLSSGLSRFPWAPQRSAYSSCKAAVNML
                     MANLRVELAQQGFTDIKATTVLPGIVATDFGLNARHGGMDNKKMPGAQPVEEVAEVIA
                     SVIEKPRAEAYTRPQMLELASRYYAAEDMSEIEQQFGQRR"
     gene            7329..8297
                     /locus_tag="VMZ53_09425"
     CDS             7329..8297
                     /locus_tag="VMZ53_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS28720.1"
                     /translation="MHRIGLVMIVGALTSSVAGAEPAKGSASSGIVTFSGTSARLSPT
                     SRETLENIAAWQRARPDALLIVDGYATRAGSRSRNLRISQQRTDVVRDALVDAGADAS
                     RLVLVAHGAENARSASTSAQRVVIRTASGFGQSSSADLADAPPAAPPPATPAAPATST
                     IVVIGGTSAANVRSASSDATTNEALANAGTHPSQSGVPGRTSGGLGTSALTGIGIDPT
                     DGVDYPSTTIASGGGATGGDTATSAPIRSGGVLPGAGTAYPGGPSATGARAGTSGAVG
                     AGVTGSGIGPTGIGTGPATASALGNATGAGTGVANGTSGGTATGGR"
     gene            complement(8303..>8934)
                     /locus_tag="VMZ53_09430"
     CDS             complement(8303..>8934)
                     /locus_tag="VMZ53_09430"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS28721.1"
                     /translation="STTGARVIGETRLVEGERVQIILEIEGVSIEVFADVVRTEPQNS
                     QAALEFVEVAAEARASIEQALASFIDRVRASSPPTVLVIGASPELFASLERDVAQLGR
                     AAHACATMLETVWALQDRTMVCEAAVLVSDLEAAPLGQVLQHLADQHPDVRRLVLFGD
                     QLSSLDHDASSRVHAVLRTPLRIRPLARALGVNLTDSSQSIKVIADPDD"
BASE COUNT         1576 a         3093 c         2910 g         1355 t
ORIGIN      
        1 ccgccgagtt gcgcgggcag ctgcgcgagc tcacggtcta cttctccgat ctcgccggct
       61 tcacatcgct ggccgagacg cgccgccccg acgagctcgt gacattcctg tccgagtact
      121 tcgacgacat gagcaagatc atcgcggccg agcgcggcac cgtcgacaag tacatcggcg
      181 acggcatcat ggcgttctgg ggcgcaccgc tgccgctcga gcagcacgcc gtgcacgcgt
      241 gtatcgccgc gctgcgctgc caagagcgcg tccgcgagct ttcgaagtcc ggcgtcgagc
      301 tcgtcacgcg catcggcctc gcgacgggcg aagtcctcgt cggcaacatc ggctcgacgg
      361 agcgcctcaa ctacacggtg atgggcgaca tcgcgaacct cgcgtcgcgg ctcgaagggc
      421 tcaacaagca gtacggaacg gatctgctga tcagcgagaa gacgtacgag caagctcgct
      481 cgaagatcgt cgcgcgtcca ctcgatgtcg ttgcggtgaa gggcaagaag gaaggcgtgc
      541 gcgtcttcga gctgctcgcg atccgcggcg aggacaaagc tgccgaagcg atcgaagatg
      601 cgtcgacgcg cgcgctgacc gcgtacctcg cccgcgactt cgcgacggca atcgccgcat
      661 ggaacgaagt cctcgcgaag cgccctgcag acaaggccgc gacgacgatg cgcgaccgcg
      721 cgcaagcctt tgctacaacg ccgccgcctc ccgaatggac cggcgtgatg atcgccaaag
      781 aaaagtaaag catgccgccg agcaccgctc ggccacgcca acccccatcg atgcgcgagg
      841 agcaaggggc caagccaggc ccctttgcgg tttccctgcg aaccatgacg gtgctatcga
      901 tggcgcgcat gaagacttcg ctcgttgcga tttcgttggc cctcgtcgcg tgcggcagcg
      961 acgcgaagac acttcctccc acggatccgg cagagctgca gacgacactc gacgcgctcg
     1021 cggcattcgg ccagaaacag ccgggcacgc cggcgggcga gcaagcggcc gcgtacatcg
     1081 gtgatcggtt tcgcgcgctc ggcctcaccg acgtgcacga cgagaagttc acgctgccag
     1141 cgtggagcgc gacgaagtcg ttcacgctct cgatcgatgg cgtgcagtcc acgccgggct
     1201 tcgacgtgtt cgaggcgtcg agcagcggca cggtgaacgc cgtgatcgta gacgtcggca
     1261 cggcgatgga tgcggagctc gagggcaaag acctgaccgg caagatcgca ctcgtgaagc
     1321 gcgagacgag ctttcatcgc tcggcgcagc tgcgcaacgt cgcggcgcat ggcgcgctcg
     1381 cgatgctcta tctgtcggtc gcgcccgaga acctgcgcca agtcggctcc gtccgcttcg
     1441 gctggaatgc gatggagccc attcccgcga tcacgatcgg tgccgacgac ggcgcgacga
     1501 tcaaggcagc gctcgccgcg aacaaggtgg tccccgcggc gatcgacgtc caagcgacaa
     1561 cgacgcccgc gagcggcatc aacgtcgtcg gcaagattcc cggcgagcgg cccgagatga
     1621 tcgtgatggg cgcgcattac gactcgtggt tcaccggctc gtcggacaac ggctcgggtg
     1681 tcgccgagct gctcgctgtc gcgtcgcgcc gcatgcaacg aggcaagccg cgctacacgc
     1741 tcgtgttcgt cgcatacgac ggcgaagaga cgggcctcta cggcggctac gactactacc
     1801 gcaagcacaa gatcgtcggc ggcgattcga tcctcgcggt gctcaacttc gagagcccgt
     1861 ccgcactcaa ccccgacatt gcggggctcg cgcactcgaa ccagcccaag ctcgacgagg
     1921 cgctgcaggc cgcgcacctt cgccagatct acgcgctcta cacgggcatg gagatcgtcg
     1981 cgcagctatt cggcggcatc atcccgacgg acatccaagg cgcgtatcgc ggcggcacgc
     2041 caaccgcgtc caccgccgtg acgaatccct actaccacac ggtcaaagac acgccggaca
     2101 aggtcgacct cgcgctgctc gtcgagtcca ccgacgcgtt cgacgcagcg atcgataacc
     2161 tcggcaagct gacgccggaa gactttgcgg tcgccgatcc gcagctctgg accgcggagg
     2221 tcgcagtgac gggcaccacc gtcgacgtca cggtgaagac cggcagcggc gcgcccggcg
     2281 ccaacgcaaa ggtcgaggcc gcggtcatgc acgacgactt catgctcgac ggcgaggtca
     2341 acgcgacgac ggacgctagc ggccacgtca cgctcacgat tcccgcgagc accggcgcga
     2401 agcgcttcct ccacgtgacg gccggtcccg tctacccgct cgccgaaacg atcatcgcgc
     2461 tgccgtagcg catcgctcgc acgccacgac tcgatatatc gcgctgccgt agcccatcgc
     2521 tcacgcgccg cgactagatg tcgatgtact cagtcatccg tgcggtactt cggatcgagc
     2581 tggttcatct gcacgacgcg tccgagctcg aacagcatcg tagagtcttc gtcggcgggc
     2641 gcgatcgcgc gagcctctag caagagctcg accgcgcggc gatagcagat cgtcgcgtgc
     2701 accggatcgc cactcgccgt gagggtcttg ccctcttccc agagcagctt cacgccgagc
     2761 cgcatcttgt cggggccgcc gagcaagctc gcgagcgtcg ccgtatcgac gacgtcgacg
     2821 atcgagcgcg ggtggccgag catgccttcc cacgcgtcgg ccgttgctac gagcgcctgc
     2881 cggtattcgc ccttctcacg caggccagcc agcttcgcga ggaattcggc gaactgccgg
     2941 atgagcctgt ggatgtagtc ctcgcgctcc atgccgcctt ggtagtcacc gccggcgcat
     3001 cacacaagtt cgcgatggcg aaacgccccg gaaacatcgt tcatgtcatg gtggtcgtat
     3061 gggtggccgc gcccgctcga tcattcacgt ccaccaccgt ggcgatcgcg gtgtcgacgg
     3121 catcctgcgg ctcatcgagc tcgcgagcca cgacggcccc ctcgaggcga tgctcaccgc
     3181 gatgtgcgac gagatcgcgt cgatcgcggg tgtcgaaatc gcgagcgtct acgtcgccga
     3241 ggacgagcgc ctcgtcatgc gcggcaacca cggcttcgac gaatccgcga tcgggtccgt
     3301 ctcgctcggc gtcggcgagg gcattacggg cctcgtcgcg gagtgcatgc gcccggtttc
     3361 cgccgcacac gcggcgaccg aagctgcgta caagcacgtg cctggcctcg gcgaggagcg
     3421 cttccccgtc tttgcgggca tcccactgat caccggcggc gctgtcgtcg gcgtgctcgt
     3481 gatgcagcgg cgcaagaagc cgttcgcgct agacgaggtc acgctcgcca ccgcactcgc
     3541 cgcgccgatc acgctcgcga tcgaacgccg caaggcgacc gccgttcgct ccgcgcgcct
     3601 caccggtgtc ggccacggcg gcgccgtcgt gctcggccgc gcgggcatcg tgccaacggc
     3661 caccgcgatc ggcaccgcgc cgttcgcatc gctgatcgcg ccgcacggtc cggcatcacc
     3721 gagcgactcc tcggatgaca tccgcgacgg cgataatccg attgcagcgg tcatcgatcg
     3781 cgtgctcgcg cgcatccgcg atgacctcgg gcgcgcgatg aagaagctcg gcgatatcga
     3841 agatcctgtg atcggtgcgt gcctcgatcg ctacgcgctc gcgctcgtcg atggtcgact
     3901 gcgcgaacgc atcatcgcgg cggcgcaaga gccatcgggg cttcgcagcg tcgcgaagga
     3961 ctacgcgcgc gcggcgactc gcatcgcgtc gcgccgccct ccgacgacac ccccgagctc
     4021 gctgagccaa gactcgcgac cacccgagct cgatgcggag acggccgagc gcgttgcgga
     4081 gatcgaagag ctgtgtgcgc tcatcgggct caccgcggat gcgcggagct cggcgcgacc
     4141 cggcgcgagc tggatcgctg accgcgtggg ccccgtgatc accgtagctg cgatcgcgcg
     4201 cggagcagcg gcggttgtcg cgagtgatcg cgtgacgccg accgcgctcg ccattgcagc
     4261 ggctgcgaag cttcccgtcg tcagcggcgt tgcgggcttg tttggctggg cgcgtccagg
     4321 ggatttgctg gcggtcgatg gcgagagcgg aacggtgctc gtgcaccccg caccgacgga
     4381 gatcgagcgt ctgcggcgtg agcggagtcc cagctagccg acggtgacct cgacggatcg
     4441 cgacatgcgc gtccggagat acagcgattt tcacagtagt cgctagtagt cacgaggagt
     4501 tacgtcggca cgcacgttgc tgctcactgg ggcatcatgc ggtttgcgtt gtacgcgctg
     4561 gttctcgtcg gatgtgggga cggcgttgac cgagatattg aaggacagat cacgatcgat
     4621 cagggcgtct acggcctgct gatttctggg tgcgatacgt cggactgcca agacaggccc
     4681 gcagcgggcg agcacgtgat ggtgtattcg ccgggactga acacggtgta cgcgaccggc
     4741 atgagcgatc gcagcggcgt ctaccagctc gatctcgagc cgggtgacta cacgctctgc
     4801 acctatgcat gcacgccgat cacggtgccc gcatcggacc gcgttcgtta cgactggacc
     4861 agcggcccgg gtggcggcca ctgggaagcc gactgatcta ctcgaccgcg ccttggcaga
     4921 accagcgccg gcgattgcga tcgtagtaga cccacaagca gtcgccgcgg cgcagctccg
     4981 cgaagtgata ctcgcgatgc agctcgtgct gccaccagcc gcccgagatg acgtacgggc
     5041 cgaggatacg gacgactgcg ccgtgctcga gacccgagag cagccagccg tcgtcgcgaa
     5101 cttgccgcgc ttgcggcggc agtagctgcg gcttcgcgta gagccggcgc acgagcggcc
     5161 gcacacgcac gggctttggc ttcgcatgca cgacgtgctg caggcgctcc cagccaaagc
     5221 tcgcttcggg caagtgaccc tcgcggagca ccgcgcgcac gacggcatca tcgccgagct
     5281 cggcacgcac gcgcgcgagc gcttcatccg ctgccttgag atcgcgacga ggcttctgcg
     5341 cgaacatcgc gagctgctcg cgcgtcgccg cgacatcgtc tgcccacacg cgtgccgcgt
     5401 tgaccggtgc aacaggtggc atcccggtga gccgcaggtg gacgaggcgt acgagcacgc
     5461 gcgcgtcgag cgtcggtacg gcgggcttga tgcaatcggc gcgcacctcc gtttggccaa
     5521 cgtgctcgcg caacgtcagc tcgacgtgca gcgccgtcag cgcgcgaccg cgtgctgcga
     5581 gccggtcgag caagcgatcg atcgcggctt tcaagaagaa gacgaggcgc tcgacatcgt
     5641 gctcgtcgtc gtcgagaacg acgtattcgt caggcgcttc cggcggcgcc atcggcacga
     5701 gcggatccca gcgctcaccc gcagcgagct gatacatgcg atgcgcatcg cgcccgaatc
     5761 tctcgaggat gccaccaccg ggaagccgca ccatctggcc gagcgtcgtc actccgaggc
     5821 gcgcgagcga atcgcgcagc ttcggatcga tatcgaggcg cgcgagcggg actgcagctg
     5881 ctgccgtgcg ttcgtccgca tcgctgcgca acaccgtcac cccgtgcctc gtcgcgcgcg
     5941 cgatcgcata cgtagcgaat cgcgagaagc cgacgaccac cgcccccgag agttgcagcg
     6001 ctgcgatcgc acgctggatc gccatgcccc acgcctgccc tttcaagccg cgcgcgccgg
     6061 gcggcgttgg cgcatccgga aaaattctgt cgagtccttc accgtcgagc cagaacacgc
     6121 ccggatcgcc cggctccacg cgcggtgaca acgcatgcag cgcggctcgc agctcgacga
     6181 tcgcagcgtc gatcttctcc ggcggcacca cgcgcgcacg caacgcgcga tgcaagctca
     6241 gcgcatgcgc gtagcgctga cctggcaaca cgcccgctgc tcgcgcgcgc tcgcacgccc
     6301 acaacacatt gccctgcgga cgatcttcat cgatgaccac gaccgcgtgc gcgcgtagct
     6361 cgggctcggt gcgccacacg agctgaagcg gcagcgccgg aagatcgaga cacgcgacgt
     6421 tcactataca agtgttcagc caaccgataa gtgctgtcaa gctcagcagt aatccggaac
     6481 cgatccccta ccatccgacg catgaaggac aaggtcatcg tcatcaccgg cgcgtctgaa
     6541 ggcatcggcg cgaccaccgc ggagctgctc gcaaagcgag gcgcccgtgt tgtccttgcc
     6601 gctcgccggc tcgacaagct cgccgcggtc cgcgcacgca ttggcgacaa tgcgctcagc
     6661 gttgtcaccg acgtcacgaa tcgcgcggat aacgagaacc tccgcgacaa ggcgatcgag
     6721 aagtggggtg gcatcgacgt gtggatcaac aacgcgggtc gcggcatctc gcgcgcggtc
     6781 gccgagctca cggacgaaga catcgacgac atggtgtcga tcaacataaa gtcggtcctc
     6841 tacggcgtgc aggccgtcct tccgcacttc aaagagaaca agcgcgggca gatcatctcc
     6901 ctctcctccg gtctctcgcg ctttccgtgg gcaccgcagc gcagtgcgta cagctcatgc
     6961 aaggccgcgg tgaacatgct catggccaat ttgcgcgtcg agctcgcgca gcaaggcttc
     7021 accgatatca aggcgacgac ggtcctgccc ggcatcgtcg cgacggactt cggcctcaac
     7081 gcgcggcacg gcggcatgga caacaagaag atgcccggtg cgcagcccgt cgaagaagtc
     7141 gccgaggtga tcgcgagcgt gatcgagaag ccgcgcgccg aggcgtacac gcgaccgcag
     7201 atgctcgagc tcgcatcgcg ctactacgcg gcggaagaca tgagcgagat cgagcagcag
     7261 ttcgggcagc gccgctagca gcgaagcgtg gcacggcggg tgctcttcct gacctcagga
     7321 ggagactcat gcatcgcatc ggcctcgtga tgattgttgg tgcgctgacc tcgtcagtgg
     7381 ccggcgcaga gccagcgaaa ggctcagcgt cgtcgggaat cgtgacgttc agcggcacct
     7441 ccgcgcggct gtcgcctacg tcgcgcgaga cgctcgaaaa catcgcagcg tggcagcgtg
     7501 cgcggcccga tgctctcctg atcgtcgacg gctacgccac ccgcgcaggc tcgcgctcgc
     7561 gaaacctccg gatcagccag cagcggacgg acgtcgtccg cgatgcgctc gtcgacgcag
     7621 gtgccgacgc gtcgcgtctc gttctcgtcg cacacggtgc agagaacgcg cggtccgcga
     7681 gtacctccgc gcagcgcgtc gtgattcgca ccgcgagcgg ttttggacaa tcctcgagcg
     7741 ccgatctcgc agatgctccg cccgcggcgc cacctcctgc aacgcccgca gcgccggcaa
     7801 catcgaccat cgtcgtgatc ggcggaacct ccgccgcaaa cgttcgcagc gccagctcag
     7861 acgccacgac caacgaggcg ctcgccaacg ccggcaccca tccctcgcag tcgggcgtcc
     7921 ccggtcgcac gagcggtggc ctcggcacga gcgcgctcac aggcatcggc atcgatccga
     7981 cggacggcgt ggactatccg agcaccacga tcgcatcggg tggcggcgcg accggcggcg
     8041 atacggcgac gagcgcgccg attcgcagcg gcggcgttct tcccggtgca ggcaccgcat
     8101 atcccggcgg ccccagcgcg acgggtgcgc gcgcaggaac gagcggcgcg gtcggcgcgg
     8161 gcgtcaccgg ctcgggcatc ggcccgacag gaatcggaac cggcccagcc acggcgagtg
     8221 cgctcggcaa tgcaacaggc gcaggaaccg gcgttgcaaa cggtacgagc ggcggcacgg
     8281 caaccggcgg tcgttagcaa ggctagtcgt cggggtcggc gatcactttg atcgactgcg
     8341 agctgtccgt caggttcacg ccgagcgcgc gggcgagagg acgaatacgc agcggcgttc
     8401 gaagcaccgc atgcaccctg ctcgacgcgt cgtgatcgag cgacgacagt tgatcgccga
     8461 acaacacgag acgtcgcacg tcgggatgct ggtcggcgag atgctgcaac acttggccga
     8521 gcggcgccgc ttcgagatcg ctcacgagca ccgccgcttc gcacaccatc gttcgatcct
     8581 gcagcgccca caccgtctcg agcatcgtcg cgcacgcatg cgccgccctg cccagctgcg
     8641 cgacatcgcg ctcgagcgac gcgaagagct ccggcgacgc gccgatcacg agcaccgtcg
     8701 gcggcgatga cgccctcacg cgatcgatga agctcgcgag cgcctgctcg atggaggcac
     8761 gcgcctcggc agctacctcg acgaactcga gcgcggcttg cgaattctgc ggctcggtcc
     8821 gcacgacatc cgcgaacacc tcgatcgata cgccctcgat ctcgaggatg atttgcacgc
     8881 gctcgccttc gacgagcctc gtctcgccga tcacgcgtgc gccggtcgtc gaca
//
LOCUS       DATLBZ010000181         7970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_919433, whole genome shotgun sequence.
ACCESSION   DATLBZ010000181 DATLBZ010000000
VERSION     DATLBZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919433"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VMZ53_09435"
     CDS             complement(<1..936)
                     /locus_tag="VMZ53_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28722.1"
                     /translation="MALNAKRLTPYWPVAILLAIAFFIAWAFAATTLANAGSMGLPLD
                     DSYIYLTYAKQFGRAQPFTYYPGGGYSAGSTSVLWPMVLAPFWTLGARGHALVWVSFG
                     VCAALYACVAVGVYRFVRKVRGCKPAGINAAAMLLVIPPFAFCALSGMEVAFAAALLV
                     ASLLLLVDQSPDGPPTKRLVACLAAASLSRPEAMLLVGAIVGIAVLARLRKRAWKPAA
                     WWLLPLVPPIVWLLANRLIAGNFFPNTGVAKSHFYLPGFDWVYWKLTVIDLTKRMFRG
                     LFWEKTSPLLWTRVVLALFLFGSARIAWWAWREKKF"
     gene            complement(927..1778)
                     /locus_tag="VMZ53_09440"
     CDS             complement(927..1778)
                     /locus_tag="VMZ53_09440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUS28723.1"
                     /translation="MDIHSSASKTALMVCAYRARASKWEKPLFVDPWAAQIAGTEGDD
                     IAKRLDQRFPPMELWLALRVAYLDRLVGLAVDKLSIRQVVILGAGYDTRAARLPRAGV
                     TFFEVDHPATQEAKRQQLAKLDNYPVDSARYVTCNFEREDPIERLLANGFSATEPALV
                     IWEGVVPYLTEGAVRTTATRLATQLDPRSLVAFDFVGKKLAVGDGLNEKDKLTREYVG
                     ELGEPIRYGSDDILPLLSECGFRWVRSLDFNELALEMIGDYKRDRMFRFQHLAIASVR
                     TPVAQWP"
     gene            complement(1803..2462)
                     /locus_tag="VMZ53_09445"
     CDS             complement(1803..2462)
                     /locus_tag="VMZ53_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HUS28724.1"
                     /translation="MSITRRILESAQSGLSRLTSLVIVDDDPLSHVESTALQAELTAR
                     KAARERSSRKPEDNPLAKLAMANPAARAQRDKATRERVARVHRERDDREAKQRAAADE
                     AFRKMKEQAARGGPMPGSSWSSTSSSSTGSSSTSSGAGPRPPRPGSHDAQVAEWYRQL
                     DLQPGADMAQIKSSYRQLMRKYHPDMHAGNPQRQKAATELSMRVTAAYNGLVTHLEQK
                     K"
     gene            complement(2479..3450)
                     /locus_tag="VMZ53_09450"
     CDS             complement(2479..3450)
                     /locus_tag="VMZ53_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28725.1"
                     /translation="MALDGILADFLSDCDHLFRMEVRMRSMYLAAACVSLIACTVDAP
                     DTSVTNQEIRTRNRLASNRLASNRLASNRLASNRLASNSLSSTILLANPETNDLMLTE
                     EGRDLYSYIVGCALPEGITIEGNVPGAPNAAPPDFNYTCSGGHCVFSGALGLAPRWAE
                     HKLDNKGQGWVSACLLSRVNANGIAEGISMRGRNDELAVSGDELVLFPAEEGAFYGNV
                     FEDVPAENDDAWLALTTNACSGEDNFVAHDQARDCATPNPATDNTLTYCGFHYAGDCR
                     DFTPAFPNPYACRGFSGSPDGYYMDCHRDAGGKGKKSREVITTYVAQ"
     gene            complement(3455..4675)
                     /locus_tag="VMZ53_09455"
     CDS             complement(3455..4675)
                     /locus_tag="VMZ53_09455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28726.1"
                     /translation="MSDSRNRKPMAPGMDALMATAVQAPTVTGYRVDRLVGEGGFGQV
                     WRATREEDGRLVAIKILHLELIRSNDALTRFERELRAIERLQHRNVVRALDEGTLDDG
                     RPYLVLEYIEGPSLRDVIHERGSLPPSEMLAVLQPLCHALAEAHKLGLVHRDVKASNV
                     ILGHDTQGPRPVLLDFGLVKLLDAEGPGLTSSRSMLGTPAAMAPEQMKGQPVTARTDV
                     YAVALLAYHMLTGAPAFGGTPGVVQSYLQVHGPRPRPSAKVDIDPAIDEPIIRALAPD
                     PSQRYATPIELLDALKAVIQPTATPDGEQEVITLYVEGGPAELAQASSIATGAGMTIA
                     MTAPDSIIAVAPRLDLTTVRARLEELASSAKVAVGSSRASICAGVVDGPALDVEGWAP
                     YPLATGLWITPTLL"
     gene            complement(4746..>7970)
                     /locus_tag="VMZ53_09460"
     CDS             complement(4746..>7970)
                     /locus_tag="VMZ53_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28727.1"
                     /translation="ADGSIVILDLGLARKLPTDPDDPTRANVQVGSLEYMPPEQIADS
                     AAVDERSDIYAFGCVLYELCAGRPPFVGDAQVLERSHTALRPPRLNAIVTVPAAVDAL
                     VNDCLAKDPARRPASVAEVRERLAVSRDERTPPQMSRSMSVIREGKQPVVLLWAELPK
                     VDRALLGMLTGRRLVVVSQRGRKILAAVLGGEHSDPAAIAIGAARDLAAAGARVGLHL
                     DALKVTQSGGTTTLHGEPIEKPETWLPSASWTGIVLTSAVASVTQAPTRAVEELGPQF
                     RALTDDKEEIEIVGREALLVDLMGDAAVALHGLPRSTTEAVSGGGRARRGSSMSGVVG
                     PRGPGFALLVGDAGVGKTAFVRELGRRMRELGVRVHVGSVPAPGTGKPSASALADLIA
                     LPAGPSSVRVIGDALRTAAREQPTAVLLDDLHLADTDLLDALEYATLGGESLALWVIG
                     VASPRIDTRRPNLGARAERQRRDLLAPLDEDSAVALTAALLRPAEYPPLRALRRLAGI
                     AHGNPLHLVMLAREIHQRGAIRERAGGQYFLDTSALDELSPAALGPWLAARELAGLGN
                     ELVAFARICAVLGGEMLREELLAVVEAVERAGGATTTIDVDVGLRELVTAGILDHIEA
                     GYVFRQPLVEEGIYATTNEDERLGIHRAALDEWRGRVGSPAVAERIARHAEAANEHAI
                     AALAFATLGRQALREQRFLDADTAWTGALRHDDVRDANRAHALIGRAQARYRLQRMHD
                     ALIDLEEAAAIASELGDVPLEVEALVEQGVVLDFIEGIAGDFARSKELAVRARARLGD
                     LAATHPGLAIDLDLADARTLFREQKFTEGAPLLREVLAKARAMGRHETATIAALLLGP
                     MLSDLRELDEAERVFGEMIADCVARADRWHLAAAYGNRAWLWSARGEIDRTEQDLNAV
                     IQLARESGQAHFERVAAHNLAEHKLWRAQLDDALGLARRGLSLQSQAAEGSTRPDRML
                     LARVFAARGELAELSEVLATFDREDDLADEERVTIAILRAIAAGDRDALAAALSGLDG
                     VFAQMRLELASLAARQGALPPELRQGLIELAKTDPLWAARIGEL"
BASE COUNT         1359 a         2686 c         2622 g         1303 t
ORIGIN      
        1 gaacttcttc tcgcgccacg cccaccacgc gatgcgcgcc gagccgaaca ggaacagcgc
       61 gagcacgacg cgggtccaga gcagcggcga tgtcttctcc cagaacaggc cgcggaacat
      121 cctcttcgtg agatcgatga cggtcagctt ccagtacacc cagtcgaagc ccggcagata
      181 gaagtggctc ttcgcgacgc ccgtgttcgg gaagaaattg ccggcgatga gccggttggc
      241 cagcagccac acgatcggcg gcacgagcgg caggagccac cacgcggcag gcttccatgc
      301 gcgcttgcgc agacgcgcga gcaccgcgat gccgacgatc gcaccgacga ggagcatcgc
      361 ttccgggcgc gacaatgacg cggctgcgag acacgcgacg agccgcttcg tcggtggccc
      421 gtccggcgat tgatcgacga gcagcaacag cgatgcaacg agcagcgccg ccgcaaacgc
      481 gacctccatt ccggacagcg cgcagaacgc gaacggcggg atcacgagca gcatcgcagc
      541 cgcgttgatg cctgcaggtt tgcagccgcg aaccttgcgc acgaaccgat agacgccgac
      601 cgcgacacac gcgtagagcg cggcgcacac gccaaacgag acccacacga gcgcgtgtcc
      661 ccgagcgccg agcgtccaga acggtgcgag caccatgggc cacagcacac tcgtcgagcc
      721 cgccgagtag ccgccgcccg gatagtacgt aaacggctgc gcgcgcccga attgcttcgc
      781 gtacgtcaga tagatgtagc tgtcgtcgag cggcagcccc atcgagccgg cgttcgcgag
      841 cgtcgtcgcg gcgaatgccc acgcaataaa gaacgcgatc gcgagcagga tcgcgaccgg
      901 ccagtatgga gttaagcgct tcgcgttcag ggccactgtg cgacgggggt acgcacggag
      961 gctatcgcga gatgctggaa gcggaacatg cgatcgcgct tgtagtcgcc gatcatttcg
     1021 agcgccagct cattgaaatc gagcgagcga acccagcgga atccgcactc cgagaggagc
     1081 ggcaggatgt cgtcggagcc atagcgaatg ggctcgccga gctcgccgac gtactcgcgc
     1141 gtgagcttgt ctttctcgtt gaggccgtcg ccgaccgcga gcttcttgcc gacgaagtcg
     1201 aacgcgacga gcgagcgtgg atcgagctgc gtggcgagcc gcgttgcggt cgtgcgcacg
     1261 gcgccttcgg tcaggtacgg cacgacgcct tcccagatca cgagcgcagg ctcggtcgcc
     1321 gagaagccat tcgcgaggag tcgctcgatc ggatcttcac gctcgaagtt acaggtcacg
     1381 tagcgcgccg agtcgacggg atagttgtcg agcttcgcca gctgctggcg cttcgcttct
     1441 tgcgtcgccg gatgatcgac ctcgaagaac gtcacgcccg cacgaggcaa gcgagccgct
     1501 cgtgtgtcgt aacccgcgcc gaggatcacg acctggcgga tcgacagctt gtcgacggcg
     1561 agcccgacga gacgatcgag gtacgcgacg cgcaatgcga gccacagctc catcggcggg
     1621 aagcgctgat cgagacgctt cgcgatgtcg tcgccctcgg tgccggcgat ttgcgcggcc
     1681 cacggatcga cgaacagcgg cttctcccac ttgcttgcgc gcgcgcgata cgcgcagacc
     1741 atgagtgcag tcttcgacgc ggagctatgg atatccacgt cgcgcttgta gcacggggcc
     1801 ggttacttct tttgttccag atgtgtgacg aggccgttat acgcggcggt cacacgcatc
     1861 gagagctcgg tcgccgcctt ctgcctctgc gggtttccgg cgtgcatgtc cgggtgatac
     1921 ttgcgcatca gctggcggta cgagctcttg atctgagcca tgtcggcgcc cggctggaga
     1981 tcgagctgcc ggtaccactc ggcgacctgc gcgtcgtgcg aaccagggcg aggcggacgc
     2041 ggaccagcgc cgctgctggt cgacgacgag cctgtcgagc tgctgctggt cgacgaccac
     2101 gacgagcccg gcatcgggcc accgcgtgcc gcttgttcct tcatcttccg gaacgcttca
     2161 tcggcggcag cacgctgctt tgcctcgcga tcgtcgcgct cgcggtgaac acgagcgacc
     2221 ctctcgcgcg tcgccttgtc gcgctgcgca cgtgccgcgg gatttgccat cgcgagcttc
     2281 gcgagcgggt tgtcttcggg cttgcgcgac gagcgctcgc gagctgcctt gcgcgcggtc
     2341 agctccgcct gcagcgcagt gctctcgacg tggctcagcg gatcatcgtc gacgatgacg
     2401 agagaggtca gccgcgacag cccggattgg gcgctctcga ggatgcgcct cgtgatgctc
     2461 acgccatgaa acgtagcatc actgcgcgac gtaggtcgta atgacctcgc gcgacttctt
     2521 gcccttgccg cccgcgtcgc ggtggcaatc catgtagtag ccatccggtg agccgctgaa
     2581 gccgcgacac gcgtacggat tcgggaatgc aggcgtgaag tcgcggcagt cgccggcgta
     2641 gtggaagccg cagtacgtca gcgtgttatc ggtcgccgga ttcggcgtcg cgcagtcacg
     2701 tgcctggtcg tgcgcgacga agttgtcttc gcccgagcac gcgttcgtcg tcagcgcgag
     2761 ccacgcgtcg tcgttctctg ccggcacgtc ttcgaacacg ttgccgtaga acgcgccttc
     2821 ttccgccggg aagagcacca gctcgtcacc ggagacggcg agctcgtcgt tgcggccgcg
     2881 catcgagatc ccttcggcga taccgttcgc gttcacgcgc gagagcaaac acgcgctgac
     2941 ccagccttgg cccttgttgt cgagcttgtg ctctgcccaa cgtggcgcga gaccgagtgc
     3001 gccgctgaac acgcaatgtc cgcccgagca cgtgtaattg aaatcaggcg gcgccgcatt
     3061 gggcgcaccg ggcacattgc cctcgatcgt gatgccttct ggcagcgcgc agccaacgat
     3121 gtagctgtag aggtcgcgac cctcttccgt cagcatgagg tcgttcgtct cgggattcgc
     3181 gagcaggatc gtcgacgaca gcgagttcga agcgagacgg ttcgatgcga ggcgattcga
     3241 tgcaagacgg ttcgatgcga gacgattcga cgcgagacgg ttgcgcgtcc ggatctcttg
     3301 attcgtcacg gaggtgtcgg gcgcatcaac ggtgcacgcg attagcgaga cacacgctgc
     3361 tgctagatac atcgaccgca tacgtacctc catgcgaaac aaatgatcgc aatcgctgag
     3421 gaagtccgca aggatgccgt cgagcgccac ccacctacag cagcgtcggc gtgatccaaa
     3481 ggccggtagc gagcggatac ggtgcccagc cttcgacgtc gagtgcaggc ccatcgacta
     3541 cacctgcaca gatcgacgct ctgctggagc ccaccgcgac tttcgccgag gacgcgagct
     3601 cctccaatcg agcgcgcacc gtcgtgaggt ccaagcgagg ggcgacagcg atgatgctat
     3661 ccggggccgt catcgcgatc gtcatccctg cacccgtagc gatgctcgat gcttgcgcga
     3721 gctcggcggg tccgccctcg acgtagagcg tgatgacttc ctgctcgccg tcgggcgttg
     3781 cggtcggctg gatcactgcc tttagcgcgt cgagcagctc gatcggcgtc gcgtaccgct
     3841 ggctcggatc gggcgccaac gcgcggataa tcggctcgtc gatcgcgggg tcgatgtcga
     3901 cctttgccga cgggcgaggc cgcgggccat gtacctggag gtaggactgc acgacacctg
     3961 gcgtgccgcc gaatgccgga gcacccgtca gcatgtgata cgcgagcaac gcgaccgcgt
     4021 agacgtccgt gcgcgccgtc accggctggc ccttcatctg ctcgggcgcc atcgcggccg
     4081 gtgttccgag catgctccgc gaggaagtca gtccagggcc ttcagcgtcg agcaacttca
     4141 cgaggccgaa gtcgagcagc acgggacgcg gcccctgcgt gtcgtgaccg aggatcacgt
     4201 tcgacgcttt cacgtcgcga tgcacgaggc cgagcttgtg cgcttcggcg agcgcgtgac
     4261 agagcggttg caagaccgcg agcatttcac tcggcggcag cgatccgcgt tcgtggatca
     4321 cgtcgcgcag gctcggacct tcgatgtact cgaggacgag atacggacga ccatcgtcga
     4381 gcgtgccctc gtcgagcgcg cggacgacgt tgcgatgttg caatcgctcg attgctcgca
     4441 gctcgcgctc gaaacgagtg agcgcatcgt tcgagcgaat cagctcgagg tggagaatct
     4501 tgatcgcgac gagccggcca tcttcttcgc gcgtcgcacg ccacacctga ccgaagccgc
     4561 cttcgccgac caaccgatcg acgcgatagc ccgtcaccgt tggcgcctga acggctgttg
     4621 ccatcagcgc gtccataccg ggcgccatcg gcttgcgatt gcggctatcg ctcattccct
     4681 actgacaacc agcgtaactg cgtttttccg acgacgcgac gtatgcaacg tggcgcccgt
     4741 gtgggtcaga gctcgccgat ccgggctgcc cacaacggat cggtctttgc caattctata
     4801 agtccttgcc gaagctcggg aggcaacgcc ccctgccgcg cggccagact cgccagctcg
     4861 aggcgcatct gggcaaatac cccgtcgaga cccgacagcg cggctgccag cgcgtcgcga
     4921 tctccggcgg cgatcgctcg cagaatggcg atcgtgaccc gctcttcatc ggcgagatcg
     4981 tcctctcggt cgaacgtcgc gagcacctcg ctcagctccg cgagctcacc tcgtgcggca
     5041 aacacacgcg cgagcaacat gcgatcgggc cgcgtcgaac cttcggctgc ctgactctgc
     5101 agcgagagac cacggcgtgc gagtccgagc gcgtcgtcga gctgtgcgcg ccatagcttg
     5161 tgctccgcga ggttgtgcgc ggcaacgcgt tcgaagtgcg cttgcccgct ctcgcgcgcg
     5221 agctgaatga ctgcattgag atcctgctcc gtgcgatcga tctcgccacg cgccgaccat
     5281 agccatgcgc gattgccgta cgccgcagcg aggtgccagc gatccgcgcg tgcgacgcag
     5341 tccgcgatca tctcgccgaa cacgcgctcg gcctcgtcga gctctcgcag atcgctgagc
     5401 atcggcccga ggagcaacgc cgcaatcgtc gccgtctcgt gacgtcccat cgcacgcgcc
     5461 ttcgcgagca cctcgcgcaa cagaggcgcg ccctccgtga acttctgctc gcggaacagc
     5521 gtgcgcgcat ccgcgagatc cagatcgatc gcgaggccag ggtgcgtggc agccagatcg
     5581 ccgagtcgcg cccgcgcacg caccgcgagc tccttgctgc gagcgaaatc gccggcgatt
     5641 ccttcgatga aatcgaggac gacaccctgc tcgacgagcg cttcgacttc cagcggcaca
     5701 tcgccgagct ccgatgcgat cgcggcggcc tcctcgagat cgatgagcgc gtcgtgcatc
     5761 cgctgcaggc gatagcgtgc ttgggcgcgc ccgatcagag cgtgggcgcg attcgcatcg
     5821 cgaacgtcat cgtggcggag cgctcctgtc catgcggtgt cggcgtcgag aaatcgctgc
     5881 tcgcgcaacg cctgacggcc gagcgttgca aatgcaagcg ctgcgatcgc gtgctcgttt
     5941 gcggcctcgg catgacgcgc gatgcgctcc gcaaccgccg gcgagccgac tctgccacgc
     6001 cattcgtcga gagcggcgcg atggatcccg aggcgctcgt cttcgttcgt cgtcgcgtag
     6061 atgccttcct cgacgagcgg ctggcgaaac acataaccgg cttcgatgtg atccaggatg
     6121 cccgcggtga cgagctcgcg gaggccgacg tcgacatcga tcgtggtcgt cgcgccgccc
     6181 gcacgctcga ctgcttcgac aacggcgagg agctcttcgc gcagcatctc gccgccgagc
     6241 accgcgcaga ttcgagcgaa cgcgacgagc tcgttgccaa gacctgcaag ctcgcgcgcg
     6301 gcgagccagg gacccagcgc tgcaggcgag agctcgtcga gcgcgctcgt gtcgaggaag
     6361 tactggccac ccgcgcgttc gcggatcgca cctcgctgat ggatctcgcg cgcgagcatc
     6421 acgaggtgca gcgggttgcc gtgcgcgatt cccgcgagcc ggcgcagcgc gcgcagcggc
     6481 ggatactccg cgggcctcag cagcgctgcc gtgagcgcga ccgccgaatc ttcgtcgaga
     6541 ggcgcgagca gatcgcggcg ttgtcgctcc gcacgcgcac cgagatttgg cctccgcgta
     6601 tcgatgcgtg gcgacgcaac gccgatcacc cacagcgcga gtgactcgcc gcccagcgtc
     6661 gcgtactcga gcgcatcgag tagatcggtg tcggcgaggt gcaggtcgtc gagcagcacg
     6721 gcggtcggtt gctcgcgcgc agccgtgcgc aacgcatcac cgatcacgcg caccgatgac
     6781 ggtcccgccg gcagcgcgat cagatcagcg agggcgctcg cgctcggctt tcccgtgcca
     6841 ggcgccggga cgctgccgac atgcacgcgc acgccgagct ctcgcatgcg gcgtccgagc
     6901 tcgcgaacga acgcagtctt gccgacgccc gcatcaccga cgagcaacgc gaagccggga
     6961 ccgcgcgggc cgacgacacc cgacatgctg ctgccgcgtc gcgcacggcc gccgcccgaa
     7021 acagcttcgg tggtcgaccg cgggaggccg tgcagcgcga ccgcggcatc acccatgagg
     7081 tcgacgagca gtgcctcgcg tccgacgatc tcgatctctt ctttgtcgtc ggtgagcgcg
     7141 cgaaattgtg gcccgagctc ttcgacggcg cgcgtaggtg cttgcgtgac gctcgcgacc
     7201 gccgaggtga gcacgatgcc ggtccacgat gcggacggta gccacgtctc gggcttctcg
     7261 atcggctcgc cgtgcagcgt ggtcgtgccg ccggactgcg tgaccttgag cgcatcgaga
     7321 tgcaggccca cgcgtgcgcc cgccgcggcg agatcgcgcg ccgcaccgat cgcgatcgct
     7381 gccggatcgg aatgctcacc gccgagcacc gccgcgagga tcttgcggcc gcgttgcgac
     7441 accacgacga gccggcgacc ggtcagcatg ccgagcagcg cgcgatcgac cttcggcagc
     7501 tctgcccaca gcagcacgac cggctgcttg ccttcgcgaa tgaccgacat cgatcgcgac
     7561 atctgcggcg gcgttcgctc gtcgcgcgag accgcaaggc gctctctcac ctcggcaacg
     7621 ctcgccggcc tgcgcgcagg atccttcgcg agacaatcgt tgacgagcgc atcgacggcg
     7681 gcgggaacgg tgacgatcgc gttgagccgg ggtggccgga gcgcggtgtg cgatcgctcg
     7741 agcacctgcg catcgccgac aaatggcggc ctgccggcgc acagctcgta gagcacgcag
     7801 ccaaacgcgt agatatcgct gcgctcgtcg accgccgccg aatcggcgat ctgctcgggc
     7861 ggcatgtact cgagcgagcc gacctgcacg ttcgcgcgcg tcggatcgtc tggatccgtc
     7921 ggcagcttgc gcgcgagacc gaggtccaag atcacgatgc tgccgtccgc
//
LOCUS       DATLBZ010000182        10655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_176226, whole genome shotgun sequence.
ACCESSION   DATLBZ010000182 DATLBZ010000000
VERSION     DATLBZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176226"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VMZ53_09465"
     CDS             complement(<1..434)
                     /locus_tag="VMZ53_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUS28728.1"
                     /translation="MRPLERIRNIGIVAHVDAGKTTLTERVLLYTGRIHAAGEVHDGT
                     AHTDSHAIEQKRGITILAAAATTDWQDHRIHLIDTPGHVDFTIEVERSLRVLDGAVVV
                     LDGVAGVEPQTETVWRQADRYRVPRIVFVNKLDRVGADYQRSL"
     gene            complement(651..1769)
                     /locus_tag="VMZ53_09470"
     CDS             complement(651..1769)
                     /locus_tag="VMZ53_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28729.1"
                     /translation="MGRACLVALFVVGGFCGAAEAKRAAPCECAESRTYPELDAYDVP
                     TNQRAIEIRPWDESEPIKITSLGELTPDTDYHFDNAPFLRFHTAAGPDWHPPSAPVSI
                     NASIVVRTSSPDSADQVATQTAIALWGDHGDDARFLEVFLENSAGQKAHFAMAAGNPQ
                     LCGQPLHISAGPAKITVWAIDRAGQRSEKPWTGEVNIVAVDDDPTGGECERRHHHFKC
                     GMGTAMLFILVVPVGFIGLLIGLFIVSIIRRSRIRNTPGEPLSPLFGEAVARVVAKGY
                     LIKSAIAVIVAIASLQMRDEELSIMAAVISWPFAFGWLIMLVRTRMIVSRFEAPLNVL
                     ERREDWLLVDRKLLYCGRSAFNRASQLPTASATLQRRD"
     gene            1865..2611
                     /gene="pyrH"
                     /locus_tag="VMZ53_09475"
     CDS             1865..2611
                     /gene="pyrH"
                     /locus_tag="VMZ53_09475"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HUS28730.1"
                     /translation="MAESRKPAYKRVLLKISGEALQGSGGYGISPEVLRVVAAEIAEV
                     ANLGVQMAVVVGGGNIWRGAQQGTGMDRASADYVGMLATVMNAVSLQSAIEKMGITTR
                     VQSAIPMSQLAEPYIRRRAIRHLEKDRVVIFGAGTGNPFFTTDTAAALRAMEIGADVL
                     LMAKNIDGVYDKDPKKHPDAKRYAQVSFTDALHRDLRVMDATAFALCRDNELAIAVFE
                     LATPGNIRRVVCGEQIGTIVNSNNETRFAN"
     gene            2624..3181
                     /gene="frr"
                     /locus_tag="VMZ53_09480"
     CDS             2624..3181
                     /gene="frr"
                     /locus_tag="VMZ53_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HUS28731.1"
                     /translation="MINDITTDAENGMKRAIDSFKRDLQKIRTGRANASMLDGIKVDY
                     YGTPTPVNQVATVQVVDARLITVKPWEKNMIAVIDKTIRASDLGINPVADSELVRLPI
                     PPLTQERRKELAKNVGKQTEEAKVAVRSARRDAMDMIKDAEKEKQITEDDRKKGETKI
                     QELTDRYIGQVDEIAKAKEKEIMEL"
     gene            3260..5359
                     /locus_tag="VMZ53_09485"
     CDS             3260..5359
                     /locus_tag="VMZ53_09485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28732.1"
                     /translation="MSGIPAGAGALLGKRLGKYEILALLALGGTAEIYLARVSGAAGF
                     EKYVVVKVLHDHLADDPDFVKMFLDEARIGAMFDHANIVQTYELGEYENRYYMVMEFL
                     AGLSLAMVVRRATERLPGGLMPVPLVLNIAAQACAGLHYTHERLLNGKPMNLVHRDIS
                     PQNLVINFEGGVKVVDFGIARAELRETKTKAGTIKGKFAYMSPEQCVAAVVDRRTDIF
                     ALGVIVHELLTGRRLFKRNSPYETYQAVLECAVEAPSKVNNQLDPALDDIVMKAVAKD
                     KDKRYPTAEAFGDALAKYLHSRGKGSGGGEIGRWFDEHFSQEIEEHGERMRELISGRD
                     FSVDTGVSWGEEEDKKANETRDLTGKGGAGEDLELSLQTGEIIEMRPSEASLDNFVGR
                     LGTESDEPNVDEDGNPVERTRIEMNPLEKLDQMNAQVAHNTGQQRITPVPPGSMSGAS
                     PLPAPPAGPVKAVVRTKESGKFPDLPNMPTQIADDGKAPELAATAIGAKHVDPNHGVE
                     AVGPMSSTQFAVRPSSFQKAQNATQPETAIPMGPGAPLSGPPMSGPPGTIPPPMGGPP
                     MGGQMGGGPMGAGPMGAGPMGHGGSGPIAGHGPSAPLITDYPVMPGGKRPMSTDLAPS
                     SLPNSQRSPYADAPLDPSAAANLMSPVGAQYPQTDWDRAAAMEAKALPPWKLGALFVA
                     VVGGALLLTLLIAKIFA"
     gene            5420..5818
                     /locus_tag="VMZ53_09490"
     CDS             5420..5818
                     /locus_tag="VMZ53_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS28733.1"
                     /translation="MSETVVITGGTAGVGRAFARRFARGGAKVAVLGRGLDDLRQTAI
                     DLVTQGASAALMIPCDVTNVEQVARATARIEEELGPIDVWIDADDPDDEPSARERALR
                     IGAAVGLAAIAAIGIILIAPRIKSAIKSAL"
     gene            complement(5802..6590)
                     /locus_tag="VMZ53_09495"
     CDS             complement(5802..6590)
                     /locus_tag="VMZ53_09495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS28734.1"
                     /translation="MLVRSLALTTELALAATRGTITDRGDYLVVETPDDPTYYHGNLL
                     VLPAAPQVGEVAYWVRKFETELTAKNGAIKHQTLWWDGIFGDVGAADELGAAGFAIHV
                     NAVMVTDHAPSPVAAPNGITIRPLDANEVLATADIAWAIADRHDDVYRRFYQRRANWH
                     RGLAVRGAATFWGAFDGADIVGSLGLVTLGNVARYQDVQTLTAYRKRGIATALLVAAA
                     RDAFAKGVQRVVIIAEAGTGADRIYSRVGFREVEKMATALKAPT"
     gene            complement(6590..6994)
                     /locus_tag="VMZ53_09500"
     CDS             complement(6590..6994)
                     /locus_tag="VMZ53_09500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HUS28735.1"
                     /translation="MGKEVIRVVAAVIEHEGRYLITQRQANAVLPLLWEFPGGRVEPG
                     EAESSALQREVRGRIGVDVEVGHKLGEHVHDYTGYSVQLTMYSCKLPAEARPYPATVA
                     DLRWVTSREFLDYDFPPADEKTMSKLLGLVRN"
     gene            7062..7469
                     /locus_tag="VMZ53_09505"
     CDS             7062..7469
                     /locus_tag="VMZ53_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28736.1"
                     /translation="MAIRFVEIKASENPETLNNEWVILENDGKQPFNTRGCGMTVGRK
                     GSNKKSMLGIIDPGFVLSPGERVRMCTGNPGTQAHGPPPEDGVKNYFLFLPKTYLSAG
                     AGTVLTLVLRGLPVSKAEFDPAASNGVAAAPAK"
     gene            complement(7470..8732)
                     /locus_tag="VMZ53_09510"
     CDS             complement(7470..8732)
                     /locus_tag="VMZ53_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HUS28737.1"
                     /translation="MDSRTAAKHLGIQQRSLYAYVSRGQVRSMAGAGGRPRLYSLLDL
                     ERLRVRRDARAGHGAVAAGALRWGEPVLDSAITAITPRGPAYRGHVAIDLAANGARFE
                     NVAELLWSGYLPEATTAVLWPRSALPIAQLGKLIPYGAKPLDVMQLVVRVAALADPSR
                     EDGRPDSIIARGRTLIPWLAAALAADFAPALVTRAAGAPTVAEVAARALGLDDAAADV
                     LDVALVVLADHELNASSFAARISASTDADVYACFSAALATLSGPKHGTAAELISRFAD
                     EIGSPDRARAAVRALRKKKAAPPGFGHPLYPRGDARALPLLEIARRYAEGTRASTAKR
                     ARTILAVVDEMIGGKAPADEIEAGAHPSVDVGLAALIAALGVQPPAGSGLFALARSAG
                     WLAHVLEQRAAGFLLRPRARYTGVALMA"
     gene            8843..10069
                     /locus_tag="VMZ53_09515"
     CDS             8843..10069
                     /locus_tag="VMZ53_09515"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HUS28738.1"
                     /translation="MSNAKPAASTTTSTSKPSASTTASGLDNVIFAETAISDVDGERG
                     RLIIAGADVEQLAAASTFEDAVARVLLAGGAPSERVGRSSDIRTALGQARALAWEAIE
                     QNRLGDALDAPDGMDALRASLAHLRAARTDDSAFDDFADAIAAIGAAPVFVAAWSRKR
                     AGAAPIAPDTTAGHAADYLRMTTGGAHASRAGATSGASLATGGAPGSVDAASVRGLDA
                     YLTTVIDHGMNASTFTARVVTSTASDLISAVVAAVGALKGPLHGGAPGPVLDMLDAIG
                     EPSRAEAWLLAELASGRRIMGMGHRIYRVRDPRAAVLENAVKPLASKRLELARAVEQA
                     AAKILAERKPDRPLAANVEFYTAVLLDAVGLPREQFAPTFGVGRIAGWCAHVLEQRRV
                     GRLVRPSSRYVGPMPG"
     gene            complement(10125..>10655)
                     /locus_tag="VMZ53_09520"
     CDS             complement(10125..>10655)
                     /locus_tag="VMZ53_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28739.1"
                     /translation="AAAAIATAAATAAAPTPAAASAPLAPKPAASVEPDPVFPPYIQN
                     KIKYAIVSADISPSGITGKREDGTIKHVVWEAIVGVIARRMPPEKPFEGATIIDVVSS
                     AGSTLRVAPWTKLRGGLPLANDAVERARGFVNLVAAQALGAKLDPATKLFAAGPGQAA
                     QLPTVATLATHDDRLA"
BASE COUNT         1901 a         3457 c         3587 g         1710 t
ORIGIN      
        1 agtgatcgct gatagtcggc gccaacgcgg tcgagcttgt tgacgaacac gatgcgaggc
       61 acgcggtacc tgtccgcttg ccgccacacg gtctccgtct gtggctctac gccagcaacg
      121 ccgtcgagca cgacgaccgc accgtccagc acgcgaagcg atcgttcgac ttcgatcgtg
      181 aagtcgacat ggcccggcgt gtcgatcaga tgaatgcggt ggtcctgcca atcggtggtc
      241 gccgccgcag ccaagatcgt gatgccgcgc ttctgctcga tcgcgtgact gtccgtatgc
      301 gctgtgccgt cgtgcacctc gcccgcagcg tgaatacgcc cggtgtagag gagcacgcgc
      361 tcggtcagcg tggtcttgcc agcatcgacg tgcgcgacga tgccgatgtt tcgaattcgt
      421 tcgagaggtc tcatagaggc tttccatagg gaaaagaatt gtccggtgaa gaaacgacgc
      481 agctatcccg cgcagggatg cgagtcatgc actgcagccc gcgcgggctc agtgactcac
      541 cgggcaaaag cacagccacg cgtagtcgac gcgggtctcg aaccggatat gtcggatcgc
      601 tggcatgtgc tgcctctggc cgtaggaatt aaggcctcgc cgcgaatctg tcaatcgcga
      661 cgttgcaagg tcgcggaagc agtcggcagt tgcgatgcgc ggttgaacgc ggagcggccg
      721 cagtacagga gcttgcgatc gacgagcagc cagtcttcgc gccgctcgag cacgtttagc
      781 ggagcttcga agcgagagac gatcatgcgc gtgcgcacga gcatgatgag ccagccaaac
      841 gcgaacggcc acgagataac ggcggccatg atgctgagct cttcgtcgcg catttgcagc
      901 gaggcgatcg cgacgatcac ggcgatcgcg ctcttgatca gatagccctt tgcgacgacg
      961 cgcgcgactg cttcaccgaa cagcggtgag agtggctcgc cgggcgtgtt gcgaatgcgc
     1021 gagcggcgaa tgatcgagac gatgaacagg ccgatgagga ggccgatgaa gccgaccgga
     1081 acgacgagga tgaagagcat cgcggtgccc atgccgcatt tgaagtggtg gtgacggcgt
     1141 tcgcattcgc cgcccgtcgg gtcgtcgtcg accgcgacga tgttgacttc gccggtccac
     1201 ggcttctcgc tgcgctgacc tgcgcgatcg atcgcccaca ccgtgatctt tgcggggccg
     1261 gcagagatgt ggagtggttg tccgcacagc tgcggattgc cggcggccat cgcgaagtgg
     1321 gccttctggc cggcgctgtt ctcgaggaac acttcgagga agcgcgcgtc gtcaccatga
     1381 tcgccccaga gtgcaatcgc tgtttgggtc gcgacttggt ccgctgaatc gggcgacgac
     1441 gtgcgaacga cgatcgaggc attgatcgat accggcgcgc ttggtgggtg ccagtcggga
     1501 cccgcggcgg tgtggaagcg gaggaacggc gcgttgtcga agtggtagtc ggtgtccgga
     1561 gtcagctcgc cgagcgaggt gatcttgatg ggctcgcttt cgtcccatgg ccggatctcg
     1621 atcgcacgct ggttcgtggg gacgtcgtac gcgtcgagct ccggataggt tcgcgactcg
     1681 gcgcactcgc atggcgctgc gcgcttcgcc tctgctgcgc cgcagaagcc accgaccacg
     1741 aacaaggcca cgaggcaggc tctacccatc atgggaagtc taacgttcga gggcggtcgc
     1801 acttcgtaaa acattgtcgt cgactccgac gacaatcggc agggaattgg tagagttcgc
     1861 cccgatggcc gagtctcgca aacccgcgta caagcgcgtt ctcctcaaga tttccggcga
     1921 ggcgctccag ggaagtggcg gctacgggat tagccccgag gttctgcgcg ttgtggcggc
     1981 cgagattgcc gaagtcgcga acttgggcgt gcagatggcg gtggtggtgg gcggcggcaa
     2041 catctggcgc ggcgcacaac aggggacagg gatggaccgc gcgagcgcgg attacgtcgg
     2101 catgctcgcg accgtcatga acgcggtctc gctacagagc gcgatcgaga agatgggcat
     2161 cacgacgcgc gtgcagtctg ccattccgat gtcgcagctt gcggagccat acatccggcg
     2221 gcgcgcgatt cgtcacctcg agaaggatcg cgtcgtgatc ttcggcgcgg gtactggcaa
     2281 tccgtttttc acgacggata cggctgcggc gctgcgcgcg atggaaatcg gcgcggatgt
     2341 tctgctgatg gcgaagaaca tcgacggcgt ctacgacaag gatccgaaga agcatccgga
     2401 tgcgaagcga tacgcgcagg tgtcgttcac cgacgcgctg catcgcgatc tccgcgtgat
     2461 ggatgcgacg gcgtttgcgc tgtgccgtga caacgagctc gcgatcgccg tgttcgagct
     2521 cgcaacccca ggtaacatcc gtcgcgtcgt ttgcggcgag cagatcggta cgatcgtcaa
     2581 cagcaacaac gagacgcgct tcgctaacta gaggacgaag aagatgatca acgacatcac
     2641 caccgatgcc gagaacggca tgaagagagc aatcgattcg ttcaagcgcg acctgcagaa
     2701 gatccgcacg ggccgtgcga acgcgtcgat gctcgatgga atcaaggtcg actactacgg
     2761 cacgccgacg cccgtgaacc aggtcgcgac ggtgcaggtt gtcgacgcgc gtctcatcac
     2821 ggtgaagccg tgggagaaga acatgatcgc ggtgatcgac aagacgatcc gcgcgagcga
     2881 cctgggcatc aatccggtcg ccgacagcga gctcgtccgc ctgccgattc cgccgctgac
     2941 gcaggagcgc cgcaaggagc tcgcgaagaa cgtcggcaag cagaccgaag aagccaaggt
     3001 ggcagtgcgc tcggcgcgtc gcgacgcgat ggacatgatc aaggacgccg agaaggaaaa
     3061 gcagatcacc gaggacgacc gcaagaaggg cgagaccaag atccaggagc tgacggaccg
     3121 ctacatcggt caggtcgacg agatcgcgaa ggccaaagag aaagagatca tggagctcta
     3181 gagggcctag gggcacctcg cagggcgatt agggctcgcg tcgggcggcg gcgcgcgaat
     3241 ccaggtatga tagccgcgca tgtccggtat tccggcgggg gccggggctc tcctcggcaa
     3301 gcgcctcggt aagtacgaga tcttggcgct gctcgccctc ggtggcaccg ccgagatcta
     3361 tctcgcgcgc gtcagcggtg cggcggggtt cgagaagtac gttgtcgtga aggtcctgca
     3421 cgaccacctc gccgacgatc cggacttcgt aaagatgttc ctcgacgagg cgcgcatcgg
     3481 cgccatgttc gatcacgcga acatcgtcca gacgtacgag ctcggcgagt acgagaaccg
     3541 ttactacatg gtgatggagt tcctcgcggg cctgtcgctc gcgatggtgg tgcggcgcgc
     3601 aaccgagcgc ctcccgggcg gcctcatgcc cgtgccgctc gtcctcaata tcgctgcgca
     3661 ggcatgtgcc ggtctgcact acacccacga gcgcttgctc aacggcaagc cgatgaacct
     3721 cgtgcatcgc gatatctcgc cgcagaacct cgtcatcaac ttcgagggtg gcgtgaaggt
     3781 cgtcgacttc ggcatcgcgc gcgccgagct tcgcgaaacg aagacgaagg ccggcaccat
     3841 caagggcaag tttgcgtata tgtcgcccga gcagtgcgtc gctgcggtgg tcgatcggcg
     3901 caccgacatc ttcgcgctcg gcgtgatcgt gcacgagctg ctcactggcc ggcgtctgtt
     3961 caagcgcaac tcgccgtacg agacgtatca agccgtgctc gagtgcgccg tcgaagcgcc
     4021 gagcaaagtg aacaaccagc tcgaccccgc gctcgacgac atcgtcatga aggcggtcgc
     4081 gaaggacaag gacaagcgct atccgacggc ggaagcattc ggcgacgcgc tcgcgaagta
     4141 tctgcacagc cgcggcaagg gttcaggcgg cggtgagatc ggccggtggt tcgacgagca
     4201 cttcagtcaa gagatcgaag agcacggcga acgcatgcgc gagctgatca gcggccgcga
     4261 tttctccgtc gatacgggcg tcagctgggg cgaagaagaa gacaagaaag cgaacgagac
     4321 gcgcgacctc actggcaagg gcggcgcggg cgaagatctc gagctgtcgc tgcagaccgg
     4381 cgagattatc gagatgcgcc cgagtgaagc atcgctcgat aacttcgtcg gccggctcgg
     4441 taccgaaagt gacgagccga acgtcgacga ggatggcaat ccggtcgagc gcacgcgcat
     4501 cgagatgaac ccgctcgaga agctcgatca gatgaacgcg caggtcgcgc acaacacggg
     4561 gcagcagcgc atcacgccgg tgccgcctgg ctcgatgagc ggagcatcgc cgcttcccgc
     4621 tccgccggcg ggcccggtca aagcagtcgt tcgcacgaag gagtccggca agttccccga
     4681 cctgccgaat atgccgacgc agatcgccga cgacggcaaa gcgccagagc tcgctgcgac
     4741 cgcgatcggt gccaagcatg tcgatccgaa tcacggcgtc gaggccgtcg ggccgatgag
     4801 ctcgacgcag ttcgcagtca ggccgagctc gttccagaag gcgcagaacg cgacgcagcc
     4861 ggagaccgcg atcccgatgg gccccggcgc gccgctgagc gggccaccga tgagcgggcc
     4921 gcctggcacc attcccccgc cgatgggcgg tccaccgatg ggcggccaga tgggcggcgg
     4981 accgatgggc gctggaccga tgggtgctgg accgatgggg cacggaggca gtggaccgat
     5041 cgccggtcat gggccgtcgg cgccgctgat caccgactac cccgtgatgc ccggtggcaa
     5101 gaggccgatg tcgacggatc tcgcgccgag ctcgttgccg aatagccaac gctctccgta
     5161 cgcagatgct ccgctcgatc cgagcgcggc cgcgaatctg atgtctcccg ttggcgcgca
     5221 gtatccgcag acggactggg accgtgccgc cgcgatggaa gcgaaggcgc tgccgccgtg
     5281 gaagcttggc gcgttgtttg tcgcggtcgt cggcggcgcg ctgctactga cgctgctcat
     5341 cgcgaagatt ttcgcgtaag ccgctgcgca ccggttttct cgtacgcgcg cgggcaagtg
     5401 ctttgcatcg ctgagacgta tgtcggagac cgtcgtcatc actggtggaa cagcgggcgt
     5461 cggacgcgcg ttcgcacgcc gattcgcgcg tggcggcgcg aaggtggccg tgctcggacg
     5521 cggtctcgat gacttgcgtc aaaccgcgat cgacctcgtc acgcagggcg cgagcgctgc
     5581 gctgatgatc ccgtgcgacg tcacgaacgt cgagcaagtc gctcgcgcaa ccgcgcggat
     5641 cgaggaagag ctcgggccga tcgatgtttg gatcgacgcc gacgatcccg acgacgaacc
     5701 gtccgcgcgc gaacgcgcac tgcgcattgg tgccgcagtc ggtctcgccg cgatcgcagc
     5761 gatcggaatc atcctgatcg cgccgcggat caagtcggcg atcaagtcgg cgctttgagc
     5821 gcggtcgcca tcttctcgac ttcgcgaaac ccgacgcgac tgtagatccg gtctgcgccc
     5881 gttccagcct ctgcgataat cacgactcgc tggacgccct tcgcgaacgc gtcgcgtgcc
     5941 gccgctacga gcagcgccgt cgcgatcccg cgcttgcgat acgcggtgag tgtctgcaca
     6001 tcttgatagc gcgcgacatt gccgagcgta acaagcccga gcgagccgac gatatctgca
     6061 ccgtcgaacg cgccccaaaa tgtcgccgca ccgcggaccg cgagcccgcg atgccagttc
     6121 gcacgacgtt gatagaagcg gcggtagacg tcgtcgtgtc gatcggcgat cgcccacgcg
     6181 atgtcggcgg tcgcaagcac ttcgttcgcg tcgagcggac gaatcgtgat gccgttcggc
     6241 gcagcgacgg gggagggcgc atggtcggtc accatcaccg cgttgacgtg gatcgcgaag
     6301 cctgccgcgc cgagctcgtc cgctgcaccg acgtcgccga agatgccgtc ccaccacagc
     6361 gtctggtgtt tgatcgcgcc gttctttgcg gtgagctcgg tctcgaactt ccgcacccaa
     6421 tacgcgacct cgccgacctg cggcgctgcg ggcaagacga gcaagttgcc gtggtagtac
     6481 gtcggatcgt ccggcgtttc gacaacgaga tagtcaccgc gatcggtgat cgtgccgcgc
     6541 gttgccgcga gtgcgagctc ggtcgtgagc gcgagactgc gaacgagcac taattgcgca
     6601 cgagcccgag cagcttcgac atcgtctttt catcagccgg cgggaagtcg tagtcgagaa
     6661 actcgcgcga cgtcacccat cgcaagtctg cgacggtcgc cggatacggc cgcgcctccg
     6721 ccggaagctt gcacgagtac atcgtcagct gcaccgagta gccggtgtag tcgtgcacgt
     6781 gctcgccgag cttgtggccg acctcgacat cgacgccaat gcgcccgcgc acctcgcgct
     6841 gcagcgccga cgactccgcc tcacctggct ccacgcggcc gccgggaaac tcccagagca
     6901 gcggcaacac ggcattggcc tggcgctggg tgatcaggta gcggccctcg tgctcgatca
     6961 cggccgcgac gacacggatc acctctttgc ccatcggcgg caattatcga tggatcgccc
     7021 ccgattgcaa cgcgattcaa gctgtctata ctgcgcgccc gatggcgatt cgcttcgtcg
     7081 agatcaaggc gtccgagaac cccgaaacac tcaacaacga gtgggtcatc ctcgagaacg
     7141 acggcaagca gccgttcaac acgcgaggtt gcggcatgac ggtcggccgc aaaggctcga
     7201 acaagaagtc gatgctcggc atcatcgacc cagggttcgt gctcagcccc ggcgagcgtg
     7261 tgcgcatgtg cacgggcaac cctgggacac aggcgcacgg tcctcccccc gaggacggcg
     7321 tgaagaacta cttcctcttc ctgccaaaga cgtatctctc ggcgggcgcg ggcaccgttc
     7381 tgacgctcgt tcttcgcggc cttccggtct cgaaggcaga gttcgatccg gccgcgtcga
     7441 acggtgtcgc cgccgctccg gcgaaatagc tacgccatca gcgcgacgcc cgtgtaacgc
     7501 gcacgcggtc gcaacagaaa tccagcagcg cgttgctcga gcacgtgcgc taaccagccg
     7561 gcgctgcgcg ccagcgcgaa caggccggag cccgcaggcg gctgcacacc aagcgcagcg
     7621 atcagtgcag cgagcccgac gtcgacgctc ggatgcgcgc ccgcttcgat ctcgtcggcc
     7681 ggcgccttgc cgccgatcat ctcgtcgaca actgcgagaa tcgtccgcgc tcgcttcgcc
     7741 gtcgacgccc gcgtgccctc ggcataccgc cgcgcgatct cgagcaacgg caacgcgcgc
     7801 gcgtcgccac gcggatagag tggatggcca aagccaggtg gcgccgcctt cttcttgcgc
     7861 agagctcgca ccgccgcacg cgcacgatcg ggcgagccaa tctcgtcggc aaatcgcgag
     7921 atgagctcgg ccgccgtccc gtgcttcggt cccgacagtg tcgcgagcgc agcgctgaag
     7981 cacgcgtaga catccgcatc ggtcgatgcc gagatgcgcg ccgcaaagct cgatgcgttg
     8041 agctcgtgat cggcgagcac gacgagcgcg acatcgagga catcggccgc tgcgtcatcg
     8101 agaccgagcg cgcgcgctgc aacctccgcc accgtcggcg cgcctgccgc acgcgtgacg
     8161 agcgcgggtg caaagtcagc agcgagtgcg gcggcgagcc acggaatgag tgtcctccca
     8221 cgcgcgatga tcgagtcggg tcgcccatcc tcgcgtgatg gatcggcgag cgcagcgaca
     8281 cgcacgacga gctgcatgac atcgagcggt ttcgcgccat acggaatcag cttgccgagc
     8341 tgcgcgatcg gcagcgcgct gcgtggccac agcacagctg tcgtggcctc gggcaaatag
     8401 ccggaccaca gcagctccgc gacgttctcg aagcgcgcgc cattcgcagc gagatcgatc
     8461 gcgacgtgac cgcgatacgc aggcccgcgt ggcgtgatcg cggtgatcgc cgaatcgagc
     8521 acgggctcgc cccaccgcaa cgcgccggcc gccaccgcgc cgtgtcctgc tcgcgcgtcg
     8581 cggcgcacgc gcagtcgctc gaggtcgagc agcgagtaca gccgtggccg gccgccggcg
     8641 cccgccatgc ttcgcacctg cccgcggctg acgtacgcgt acagcgaccg ctgttggatg
     8701 cccagatgct tcgccgcggt tcgcgaatcc acccactcct gctcgtcgac ccgactttcg
     8761 cgagcaatga ccgaacgttg attgttgaat ctacgttgat caaccattga ttccaccgta
     8821 catcagcagg caggaggtga ccatgagcaa cgccaagccc gctgcgtcca ccacgacgtc
     8881 cacgtccaaa cccagcgcga gcaccacggc atcgggcctc gacaacgtga tcttcgccga
     8941 gaccgcgatc tccgatgtcg acggggaacg cggccgcctg atcatcgcgg gcgccgatgt
     9001 cgaacagctc gccgccgcat cgacattcga agacgccgtc gctcgcgtgc tcctcgcggg
     9061 tggcgcccca agtgagcggg ttggccgcag ctcggacatt cggacggcgc tcggacaggc
     9121 ccgcgcgctc gcgtgggagg cgatcgagca aaaccgactc ggcgacgcgc tcgatgcgcc
     9181 cgacgggatg gacgcgctgc gcgcctcgct cgcgcatctc cgcgccgccc gcaccgatga
     9241 ctccgcattc gatgatttcg cggacgcgat cgccgcgatc ggtgctgcgc cggtgttcgt
     9301 cgctgcgtgg tcgcgcaagc gcgcgggtgc cgcgccgatc gcaccggata cgacggctgg
     9361 ccacgccgcg gactacctgc gcatgacgac gggcggtgcg cacgccagcc gcgcaggcgc
     9421 gacgagcggg gcgtcgctcg cgacgggcgg ggcgcctggc tccgtcgacg cggcgagcgt
     9481 gcgtggcctc gatgcgtatc tgacgacggt gatcgaccac ggcatgaacg cgtcgacgtt
     9541 cactgcgcgt gttgtcacgt cgacggcgtc ggacctcatc tcggcggtcg tcgctgcggt
     9601 cggcgcgctc aaaggcccgc tgcacggcgg cgcgccgggg cccgtgctcg acatgctcga
     9661 cgcgatcggc gagccatcgc gcgctgaagc gtggctgctc gccgagctcg catcggggcg
     9721 gcgcatcatg ggcatgggcc atcgcatcta tcgcgtgcgc gatccgcgtg cggcggtcct
     9781 cgagaacgcg gtcaagccgc tcgcgtcgaa gcgcctcgag ctcgcgcgcg ccgtcgagca
     9841 agcagctgcg aagattctcg cggagcgcaa gccggatcgg ccgctcgccg cgaacgtcga
     9901 gttctacacg gcggtgctcc tcgacgcggt cgggctgccg cgcgagcagt tcgccccgac
     9961 gttcggcgtc ggccgtatcg ctggctggtg cgcgcacgtg ctcgagcagc gtcgcgtcgg
    10021 ccggctcgtg cgaccgagct cgcgctacgt cggccccatg cccggctaac gaaacgtcgc
    10081 cggactacgc gaggcggtcg tcgtgtgtgg cgagcgtcgc gcgactacgc gaggcggtcg
    10141 tcgtgcgttg cgagcgtcgc gaccgtcggc agctgcgccg cttgacccgg accggcggcg
    10201 aacagcttcg tcgcgggatc gagctttgcg ccgagcgctt gcgcggcgac gaggttcacg
    10261 aagccacgcg cgcgttcgac cgcgtcgttc gcgagcggca ggccgccgcg cagcttggtc
    10321 cacggcgcca cgcgcagtgt cgagcctgcg gacgacacca cgtcgatgat cgtcgcaccc
    10381 tcgaacggtt tctcgggcgg catgcgccgc gcgatcacgc cgacgatcgc ctcccacacg
    10441 acatgcttga tcgttccgtc ttcgcgcttg cccgtgatgc cgcttggcga gatgtccgcg
    10501 ctgacgatcg cgtacttgat cttgttttga atgtacggcg ggaagactgg gtcgggctcg
    10561 accgacgcag cgggcttcgg cgcgagcggc gcagatgcgg cggccggcgt cggtgcagct
    10621 gccgtcgcag cggcagtcgc gatggcagca gcggc
//
LOCUS       DATLBZ010000183        17957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4642797, whole genome shotgun sequence.
ACCESSION   DATLBZ010000183 DATLBZ010000000
VERSION     DATLBZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4642797"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VMZ53_09525"
     CDS             complement(<1..1115)
                     /locus_tag="VMZ53_09525"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUS28740.1"
                     /translation="MSSSDENVPEPVRAGTDLSTEVSTLPPEQRAEVIAEIRAVAEQR
                     GEDSEVVELVELPATGRARRPSSITLPPMPSERATPPPEPGAKVEEEPLSPDELRDAW
                     PLLDLEERSDGLRVMPREDAEEFFIALSAHDQAKLVLHFRPGQRRQWMRMLEPDDVAD
                     VIQDAGDQHKQTFLALLDDPTRKEVLALLAYAEDEAGGLMSTRFARLRPNMTAGEALS
                     YLQRQARAKLETIYVAYVIDMDQRLHGVVSFRELFAAEPKKLVSEIMETDVVRVTEDM
                     DQETVSRLFAEHDLNVIPVVDANGKMKGIVTVDDIVDVVQEEATEDAQKFGGMEALEV
                     PYLQSSRREMIKKRIVWLIILLAGSMLTATALESFQG"
     gene            complement(1162..1983)
                     /locus_tag="VMZ53_09530"
     CDS             complement(1162..1983)
                     /locus_tag="VMZ53_09530"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HUS28741.1"
                     /translation="MSTLPDSVIATCKAALTRDGGVVVLTGAGVSAESGIPTFRGKEG
                     YWTVGAREYHPQELATHDAFSRMPWDVWAWYLYRRGVCRAAEPNAAHRAIVRWDAALG
                     ERFGLVTQNVDGLHRRAGSPAARMFPIHGDIGLMRCARDCTLDRYPIPDGVPALGKGE
                     AVDADVKAQLVCPKCGGMARPHVLWFDESYDEERFFLDTARRLAMQATLLVVAGTSAQ
                     TNLPWQVVTLAARAGATIVDINVEDNPFAEIAAESGGAVRGSAAEVFPALVDALT"
     gene            2016..2510
                     /locus_tag="VMZ53_09535"
     CDS             2016..2510
                     /locus_tag="VMZ53_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28742.1"
                     /translation="MRRPLMIAMFALAACSRASNEGEAKQWQEQPPPKEVTVPAGLSI
                     AVTVQGADRPPITSKTLESTKADFVDPEHRAWRIPTLVADATAGSTVEAASPSGVAVK
                     FGEPTPDGLEPVLFLTRRGEVIVAAVDPKDPFPRYHGQGARLKRPGDSMPRVAPVVKL
                     SITK"
     gene            complement(2511..3692)
                     /locus_tag="VMZ53_09540"
     CDS             complement(2511..3692)
                     /locus_tag="VMZ53_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28743.1"
                     /translation="MVIIWANLDAEARWANQTLPKHVLEKISAAAVTLAALTRKGKEV
                     ELWTPAPVDPARIKIKGVTVREGVPARQHIVWCKPHAKAANDRRVALAVHEKLGTLLA
                     GQRAITSLDELASATGPWVAKAPWTAAGRDRAMGNGPPTGELAVHVTRLLERCGVLMF
                     EPWCERIFDLGVCGSIDEAGAVTKYSQHTLRSDARGNFVGIALTSPTLTRVETAKLES
                     YIAEAGRALHALEYSGPFGIDAFIYRTPETELPVFIVLVEDMYEPYETRRAVFSSAHR
                     TRAEAEAAADAGRIRPHAPDEQPMEQYHVREGTLSLDAGGQWQLACALAKNESAPVEY
                     VVRDIGPGRALHVCEINARHTFGHVTWGLFTHYDANELGFGEPPEGARVLVEGGAWIK
                     R"
     gene            complement(3699..4505)
                     /locus_tag="VMZ53_09545"
     CDS             complement(3699..4505)
                     /locus_tag="VMZ53_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HUS28744.1"
                     /translation="MITARDYALAIVSGDTLDAKLVAPPADLQLEDSSEALRITAPGR
                     PANLAIVSSRDARVPPIQGMKDRSQRARILHALANHELQAIELFAWALLAYPDAPIAF
                     RRGLVAILADEQRHLALYMQRLAAHGASFGDHAVTGHFWNKLDHLTTPLEFVCAMGLT
                     FENANLDFAGDYAAAARAAGDVDTATVLDQVHTDEIAHVHFGWVWLRRLGGDGDAWAA
                     YLANIKFPLGPNRARGARFDRDARRRAGFDEAFIDKLEATLPTRPSGEPR"
     gene            complement(4502..5893)
                     /locus_tag="VMZ53_09550"
     CDS             complement(4502..5893)
                     /locus_tag="VMZ53_09550"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS28745.1"
                     /translation="MSTASQIIRIGLVLCALAPAASAERKVETYESGHYKPMRVRIPT
                     ENNSNPSNVVSSRLVYLRRCPIGAGCALKFGSTEDSRTDTSTIVNGQRIIGEFTQTDE
                     VWQAQLACVKKTFEPFNIQVTDVDPGNVPHFENIVGGKPTDLGRTDLSSAGGVAPFTC
                     GEVPNAIVFTFDVWGADAEQLCAVSAQEVAHAFGLDHEFLQKDPMTYIIGDLPKRFRD
                     VNADCGELETRPCDCAGRAKQNSYRMILNLFGPGAPTPPEVTFTSPSEGREIQPGFRA
                     VIKALDDVRIAKVELYADGMLIGMSTTEFHDKFYITGPTDLSMGPHTLEARAYDVQDV
                     MATTTISVNQGPPCTASKGCTGSEVCVSGICLPGPDEPGGLGDQCQSDAECLSQHCTD
                     GGEKFKHCTETCDPGNSGSCPNEFECLASGGTGVCWPAPDSGCCDAGTTPQGPLLLGV
                     GVLALVLRRRRRR"
     gene            complement(5872..7338)
                     /locus_tag="VMZ53_09555"
     CDS             complement(5872..7338)
                     /locus_tag="VMZ53_09555"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS28746.1"
                     /translation="MAMRRFGIVVFILVAAQVAAAEPRLRDGTLDWPDDPTFVPTGTV
                     ELAATNKLYLNRCANGCVINVGQSNSINNSWPIGAQRTLTKFPFTDANWNAVVACVKD
                     VFEPYNVVITETDPGTAGHFEIMIGGLPTDLGFDPMIGGVAPPGCNNGYLNTALVFDF
                     AKAWSYGATNCDTACIENICTTAAQEIGHTWANMDHVIDKKDPMTYFNYNRRKYFQDA
                     NQQCGSDCDAATGRGPGGVQCTGTDTQSHTCRCTGGQAQSQNSFAIIKNLFGTNAGTP
                     PMVSISSPKAGATVDKGFAIKVEAMDNSNLIAKLELKVDGTAIDTSMIDTLSMPSPWV
                     FYAPDSLANGPHHIEAMAMDPQQTRATASTDVVQNPPCNASTTCPNATDACVGDRCVP
                     GPDEPGGLGATCMFTTDCQSGQCANDGEHLYCVENCSPGDCPSGFGCLDDGQGNGFCW
                     PGYDESGGCGCQSNRPGGPLTLGLLFAVGLYTCRRRRR"
     gene            complement(7348..8502)
                     /locus_tag="VMZ53_09560"
     CDS             complement(7348..8502)
                     /locus_tag="VMZ53_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS28747.1"
                     /translation="MRSIAGVLAVVCLVSWASTAEAELDRSRFVRVPTWVKPEDVPHA
                     VNSRTIFLNRCAGGCKITFGTTDSRTNKSQIGQGTLSAYKYGDDSWNAVVACMKDTFS
                     RFNIRITDRDPGMTAPHFEIMIAGTPGQIGLSGGVLGIAEYSCQQAGVCTPFLPNTIV
                     YDFANSYDGIPNRDEEICATAAQEIAHTWTLDHVTDKTDPMTYYQYQGRRQFKDGVAC
                     GSDCVNGQSPFGLTCSGQSHTCMSTGKATQDDVKLLLGLFGPSCVDDAGCEAGYFCYE
                     KACLAGPQVTGGLGATCVTDADCLDGACANDGNMMACATTCDLNNDASCPTGFGCLDA
                     GKSDGTGVCWAGLDDGGCCDTSGRSNGAGPMLLGLGVLAAFVTRRRRKAA"
     gene            complement(8569..9945)
                     /locus_tag="VMZ53_09565"
     CDS             complement(8569..9945)
                     /locus_tag="VMZ53_09565"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS28748.1"
                     /translation="MNRTFACSAALLLGMFGTAAAAPRTAMKWVDESRLGKEWIGPDS
                     SAAVSHVIYMNNCKPNGCQLSPGSDNAPLNRSSVPDASSTVSAYTGSDANWQAIVQCV
                     KQTYADFDVQIVTERPASGNYHMAIVAGTPNQVQQQSGVLGVSPFSCGYISNSVSYTF
                     ANLEPSNVADLCWTVAQETAHSWGLDHKYDNRDPMTYLSGGPTYKRFQDAAGSCGEYS
                     ARSCQCQYNGSTAKMNSHQLIESVFGSSAPDTVAPMVNITFPMDKAQVNAGFPIKADV
                     SDDRTVEKVEIKLDGMVIKTLTDAPFNSAAPATLGQGMHKVEVIGYDHAGNKSTATVN
                     VQYGTVCTTDSECTTAGDVCVEGHCVAGPSMEGGLGTDCVGNEDCSTGQCGNDGDHSY
                     CTTSCNPEQSACPSGFACLDTGGGNGLCWPSDGGGCDAGVTHNGGQGFLLFSLGLVGI
                     LIVRRRRR"
     gene            complement(10057..11076)
                     /locus_tag="VMZ53_09570"
     CDS             complement(10057..11076)
                     /locus_tag="VMZ53_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HUS28749.1"
                     /translation="MSTVLDITLLILGIAVLYWGAEWLIRGSASMARAMGVKPLVIGL
                     TVVAYGTSAPELAVATKTALAHSQPIALGTVIGSCAANISLILGLTALIAPPTVDGRI
                     IRREVPVLVGSAIAVPILFRNGVLSRVEGALLIGCAIVFTIVTLSVSARLDLDMEDDE
                     PENAGSSASMSSAQTAQDSGLAIGGRGRPRASHLMSILMSTLGLVLLLAGSYLFVGGG
                     RRIAADLDMSDRMLGLTVISIGCALPELIGSLVAASRGQSALAVGSVIGSNLLNVFLV
                     LGVTAYLSPIHLGERMHLVDLIGLVVITLLGVLTLRGSRKITRLEGVILILAYVAFIV
                     CAAIF"
     gene            complement(11089..11739)
                     /locus_tag="VMZ53_09575"
     CDS             complement(11089..11739)
                     /locus_tag="VMZ53_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HUS28750.1"
                     /translation="MTRDRDALQRIPGPKLLLVLLGIVVAIALTIFIPSFVKRPADKQ
                     LKDLATIGQFSLVDEMNRPFTDSALHGHVTIVNFIFTRCDTICPVSTMKMERIQAKTE
                     DVAKKIKLVSFSVDPKYDTPQRLLEYATKYRYQPDRWRFVTGDFDTVHDTIERQFMTS
                     MMRLPDKPNGVPDISHQGYFLLLDKEAHLRGIYDSDRINQLDELIHDARQLARTQK"
     gene            11826..12365
                     /locus_tag="VMZ53_09580"
     CDS             11826..12365
                     /locus_tag="VMZ53_09580"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUS28751.1"
                     /translation="MWLAHALTLSRIPIAAVFWLTYGQSRALSLGLVVLAALTDAADG
                     TVARWVKHRGGSGSTLGEWLDPAADKLFIIGVLGAIQVHDPVPWSLIALIVARELLLV
                     PLAAIYRFVVHGRGEHAFKAGNIGKAATIAEIFAIGAIILHSRAVVPLAVAAAGLGIA
                     AVVSYIWRATHVRHEAAHA"
     gene            12362..12709
                     /locus_tag="VMZ53_09585"
     CDS             12362..12709
                     /locus_tag="VMZ53_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUS28752.1"
                     /translation="MKTMKTHKRGDFEILHATASSQAAMMTLPPKGKSSEGSLNEHAW
                     AEQWLYVVSGEGRVTSRKKKYPLEPGTLLLIEKGEPHEIENTGTTPLVTLNVYAPPAY
                     DDDGEPLYGGTDP"
     gene            complement(12706..13854)
                     /gene="cfa"
                     /locus_tag="VMZ53_09590"
     CDS             complement(12706..13854)
                     /gene="cfa"
                     /locus_tag="VMZ53_09590"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HUS28753.1"
                     /translation="MPASALLSQFDARRANELVRNALAEAGIVVGGSDPWDIDVKDDR
                     FYQRLLRDGTLGFGEAYMEGWWDSRELDQTIDRVCRHRLRDHVKDNWVLLAHAVKARV
                     LNPQKTRAFEIAYKHYDIGNDLYEAMLDKRMLYTCAYWRDAKDLEAAQEAKLELVCQK
                     IGLKSGMRVLDLGCGWGGFAAYAAEKYGAEVTGYTVAAEQVRWAKEKYAHLPVDIRHD
                     DYRKATGKYDAVVSIGLMEHVGPKNYRAYMELVNQLLAPGGVGFVHTITGNRTTTHIE
                     PWFDKYIFPNAVLPTPDAMINAMERLFVIEDLHNIGEHYDPTLMAWWQRFDAAWPKLE
                     AKYGATFYRMWKYYLLGCAGGFRARETQLVQLVFTRVGTKQPAGVRAR"
     gene            complement(13844..14995)
                     /locus_tag="VMZ53_09595"
     CDS             complement(13844..14995)
                     /locus_tag="VMZ53_09595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS28754.1"
                     /translation="MAELYDAVIVGGGPAGSTCARTLTSRGARVAVIDRAEFPRVKLC
                     AGWLSPTFWDVIGRAPSEYPRGLWEWHNCHVHYRGKQYTLNGHGWFIRRVELDDWLLR
                     ESGAELHLGTNVKDIVREDDGTWRVGELRAKYLVGAGGTHCPVARLLEPPRPRRAVGV
                     QELELQTDHDAIVRTRLGKDGEPELVLFDGLSGYGWNVPKKDWLNIGCGTLDATAVRD
                     AWKTTHSLLRDKGTVPDEAEPQLAHLKGHSYFLFDPAHLEGAVRGTALLVGDSLGLAH
                     PLTAEGIVPAVTSGRLAAEAILAGDPASYVQALRSDMLLRDYRRTHRVVEAARRLKNK
                     AKSNGNGGGKGRFDRAVARGFAWMFSGARLPAPKVIDFVLDRMESRHAS"
     gene            complement(15036..15989)
                     /locus_tag="VMZ53_09600"
     CDS             complement(15036..15989)
                     /locus_tag="VMZ53_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28755.1"
                     /translation="MRIAALLLVAGCGRFSFGDRPDAAVDVKPPRPPLVWHAETTPVT
                     DKLFDIWGSSATDIYAVGNGSALLHSTGNDTWTLENVPVTTALYGVGATTASDVIVAG
                     NATPSGMAILRPNGAGGWQQEPNTLPQVLNDGYVASPTNMWVIGYGGLIAHSNGDGSW
                     TSESTGTSATLFTWWASGPNDFYVVGDAGTLLRSTGDGTWTPQSTGTQAKLVGVGGSS
                     ANDVWAVGYSGKILHSTGNGVWTAQDANVGFDLYNVFTVDGFVLACGVGNTVITSEGD
                     GVWTQQQLPVPSTQICDGAWGPTADDIHLALDGGVILRGTR"
     gene            complement(15999..16589)
                     /locus_tag="VMZ53_09605"
     CDS             complement(15999..16589)
                     /locus_tag="VMZ53_09605"
                     /inference="COORDINATES: protein motif:HMM:NF018983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 75 protein"
                     /protein_id="HUS28756.1"
                     /translation="MGQPPTAQQLRDKLVACNVIGGPYAKDSGGAATVDVCGLPGAVW
                     WISEMDVDCDGVMTTKCNLSTDPAYQNQTAATDSNGDALDAAALPYVVVPGKSTRWDY
                     RAAGLQMGSVFAVVYKDSVQYGVAGDVGPTAIIGEASYRMAELLGIDPDPRTGGTDEA
                     VAYIGFVGTDGVVDPIEDHDRAVSLGIEKARALVAQ"
     gene            complement(16676..16906)
                     /locus_tag="VMZ53_09610"
     CDS             complement(16676..16906)
                     /locus_tag="VMZ53_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28757.1"
                     /translation="MSIAIQVHANDPAALADLIAQTMHNGIVIDREPTIGNLSALVAA
                     EEKDGERRRAAWKWLNSISSAQIDRDNDWSDV"
     gene            complement(16969..17811)
                     /locus_tag="VMZ53_09615"
     CDS             complement(16969..17811)
                     /locus_tag="VMZ53_09615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUS28758.1"
                     /translation="MRILASLACVLATATGCLDEADSIDEHDEGEDSALYEQAYEDGL
                     AEGKTDGTDCSGVRVPDRSGFGKRIALTFDDGPNPVTTPKVIDVLKRHGAPAAFFNNG
                     SRYSVAGAKEVAARIAADPDYILANHSQNHLNLAQQTLARVGSEIDATDALVRGAGET
                     PAYFRFPFGSSTCAGMKLARNKGYVVTGWHIDSADWCYAAGGGVCKRSTFRYVDDALR
                     DNMVGFVVAQAKANNGGILLMHDIHANTAARLDDVLTALEQQGFTFVRLDDRDAFPKL
                     HAAP"
BASE COUNT         2993 a         6020 c         5810 g         3134 t
ORIGIN      
        1 tgaccttgga acgactcgag cgccgtcgct gtgagcatcg atcccgcgag caggatgatg
       61 agccacacga tccgcttctt gatcatctcg cgtcgagacg actgcagata cggaacctcg
      121 agggcttcca taccgccgaa cttctgtgcg tcttccgtgg cctcttcctg gacgacgtcg
      181 acgatgtcgt cgaccgtgac gatacctttc atcttgccgt tggcatcgac gacggggatg
      241 acgttgagat cgtgctccgc gaacaggcgc gacacggtct cctgatccat gtcctccgtc
      301 acgcgcacga cgtccgtctc catgatctcg gagaccagct tcttcggttc ggccgcgaac
      361 agctcgcgga acgacacgac gccgtggagc cgctgatcca tgtcgatcac gtacgcgaca
      421 tagatcgtct cgagcttcgc gcgcgcttgc cgctgcaagt acgacagcgc ttcgcccgcg
      481 gtcatgttcg ggcgcaggcg tgcaaagcgc gtcgacatga gaccgccggc ctcgtcctcg
      541 gcgtacgcga ggagcgcgag cacttccttg cgcgtcggat catcgagcag cgcgaggaat
      601 gtctgcttgt gttggtcgcc ggcgtcctgg atcacgtcgg cgacgtcatc gggctcgagc
      661 atgcgcatcc actgccggcg ttgacccgga cggaagtgga ggacgagctt cgcctgatcg
      721 tgcgcggaca gcgcgatgaa gaattcctcg gcgtcttcgc gcggcatcac gcggagacca
      781 tcgctgcgct cttcgagatc gagcagcggc cacgcatcgc ggagctcgtc gggagagagc
      841 ggctcctctt cgactttcgc gccgggctcc ggcggcggcg tcgcccgctc gctcggcatc
      901 ggcggcagcg tgatcgagct tggccggcgc gcgcgtccgg tcgcagggag ctccaccagc
      961 tcgacgacct cggaatcttc gccgcgctgt tcggcgaccg cgcgaatctc ggcgatgact
     1021 tccgcgcgct gctcgggcgg cagcgtcgag acctcggtgg agaggtccgt tccggcgcga
     1081 accggctccg gtacgttctc gtccgaggaa gacatcgact gaaccctgcc ttctcggatc
     1141 taaagtgtcc agatcagagc gtcacgttag tgcatcgacc agggctggaa acacctcggc
     1201 cgcggatccc cggaccgccc caccggactc ggcggcaatc tcggcaaacg ggttgtcctc
     1261 gacgttgatg tccacgatcg tggcgccagc gcgcgctgcc agcgtgacca cctgccacgg
     1321 gaggttcgtc tgcgccgagg tccccgcgac gacgagcagg gtcgcctgca tcgccagccg
     1381 ccgggcggta tcgaggaaga accgctcctc gtcgtagctc tcgtcgaacc acagcacgtg
     1441 aggccgcgcc atccctccgc atttcgggca cacgagctgc gccttcacgt cggcgtcgac
     1501 cgcttcgccc ttgccgagcg caggcactcc gtcgggaatc gggtagcggt cgagcgtgca
     1561 gtcgcgcgca catcgcatga gcccgatgtc gccgtgaatc gggaacatcc gggccgcagg
     1621 actgccggcg cggcgatgga gcccgtcgac gttctgtgtg acgagcccga accgctcccc
     1681 gagcgccgcg tcccacctaa cgatcgcgcg atgcgcggca ttcggctccg ctgcccggca
     1741 cacgccgcgg cggtacaggt accaggccca cacgtcccac ggcattcgcg agaacgcgtc
     1801 gtgcgtcgcc agctcctgcg ggtggtactc gcgcgcaccg acggtccagt agccttcctt
     1861 gccgcgaaac gtcggaatgc cgctctcggc agacacaccg gcccccgtca gcacgacgac
     1921 gccaccatcg cgggtgagcg ccgccttgca cgtcgcgatc acgctgtccg gcagtgtgct
     1981 catgtcccga cgatagccaa tgctagactt gccgtatgcg ccgcccgctc atgatcgcga
     2041 tgttcgcgct tgcggcgtgc tcgcgcgcga gcaacgaggg cgaggccaag cagtggcagg
     2101 agcagccacc gcccaaagag gtcacggtgc cagcgggcct gtcgatcgcg gtcaccgtgc
     2161 agggcgcaga tcgaccgccg atcacgagca agacgctcga gtcgacgaaa gcggacttcg
     2221 tggatcccga gcatcgcgcg tggcgcatcc cgacgctcgt ggccgacgcg accgcgggct
     2281 cgaccgtcga agcggcatcg ccgagcgggg tcgcggtcaa gttcggcgag ccgacgcccg
     2341 acggtctcga gccggtgctc ttcctcacgc gtcgcggcga ggtgatcgtc gctgcggtcg
     2401 atccaaagga tccgttcccg cgctaccacg gtcagggcgc gcgtctgaag cgtcctggcg
     2461 attcgatgcc gcgcgtcgcg ccggtcgtga agctgtcgat cacgaagtag ctaccgcttg
     2521 atccacgcgc cgccctcgac gagcacgcgt gcgccttcag gcggctcgcc gaagccgagc
     2581 tcgttcgcat cgtagtgagt gaagaggccc cacgtgacgt gaccgaacgt gtgccgcgcg
     2641 ttgatctcgc agacgtggag cgcgcgtccc gggccgatgt ccctcacgac gtactcgaca
     2701 ggagcgcttt cgttcttcgc gagcgcacac gcgagctgcc attgaccgcc agcgtcgagc
     2761 gagagcgtgc cttcgcgcac gtgatactgc tccatcggct gctcgtcggg cgcatgcggt
     2821 cgaatccggc cagcgtcggc cgccgcttcg gcttcggcac gcgtgcgatg cgcactcgag
     2881 aacaccgcgc ggcgcgtttc atacggctcg tacatgtcct cgacgagcac gataaacacc
     2941 ggcagctcgg tctccggcgt tcgatagatg aacgcgtcga taccaaacgg cccgctgtac
     3001 tcgagcgcgt ggagcgcgcg gcccgcctct gcgatgtagc tctcgagctt cgccgtctcg
     3061 acgcgcgtca gcgtcggact cgtgagcgcg atcccgacga agtttccgcg cgcatctgac
     3121 cgcagcgtgt gctgcgagta cttcgtgacg gcgcccgcct cgtcgatcga accgcacacg
     3181 ccgagatcga agatgcgctc gcaccatggc tcgaacatca gcacgccaca acgctcgagc
     3241 aagcgcgtga cgtgaacagc gagctctccc gtcggtggcc cgttgcccat cgcacgatcg
     3301 cggcctgccg ccgtccacgg tgccttcgcg acccacgggc cggtcgccga tgcgagctcg
     3361 tcgagcgatg taatcgcgcg ctgacccgcg agcagcgtgc cgagcttctc gtgcacggcg
     3421 agcgcgacgc gccgatcgtt ggccgccttt gcgtgcggct tgcaccacac gatgtgctgc
     3481 cgcgcgggca cgccttcgcg cacggtgacg cccttgatct tgatgcgcgc cggatcgacg
     3541 ggcgcgggcg tccacagctc gacttccttg cccttgcgcg tgagcgcggc gagcgtgacg
     3601 gccgcggcgc tgatcttctc gagcacgtgc ttcggcagcg tctggttcgc ccagcgcgct
     3661 tccgcgtcga ggtttgccca gatgatgacc atcgcccgtc agcgcggctc gccactgggg
     3721 cgcgtcggca gcgtcgcctc gagcttgtcg atgaatgcct cgtcgaagcc agcgcggcgg
     3781 cgcgcgtcgc gatcgaaacg tgcgcctcgt gcgcggttgg ggccgagcgg aaacttgatg
     3841 ttcgcgagat acgcggccca cgcatcgccg tcgccgccga gccggcgcag ccacacccag
     3901 ccgaagtgga cgtgcgcgat ctcgtcggtg tgaacttggt cgagcacggt ggcggtgtcg
     3961 acgtcaccgg cggcacgcgc ggcagccgcg tagtcacccg cgaagtcgag gttcgcattt
     4021 tcgaacgtca ggcccatcgc gcagacaaac tccagcggcg tcgtcaggtg atcgagcttg
     4081 ttccagaagt gaccggtcac ggcgtggtcg ccaaacgatg cgccgtgcgc ggcgagccgc
     4141 tgcatgtaca gcgcgagatg gcgttgctcg tcggcgagga tcgcgacgag cccgcggcga
     4201 aatgcgatcg gcgcatcggg gtaggcgagc agcgcccacg cgaacagctc gatcgcctgc
     4261 agctcgtgat tcgcgagagc gtggaggatg cgcgcgcgct ggctgcgatc cttcatgcct
     4321 tggatcggcg gcacacgcgc gtcgcgcgac gagacaatcg cgaggtttgc tggccgtccc
     4381 ggcgcggtga ttcgcagcgc ctcgctcgag tcttcgagct gtagatcagc cggtggcgcg
     4441 acgagctttg cgtcgagcgt gtcgccgctg acgatcgcga gcgcgtagtc gcgtgcggtg
     4501 atcatcgacg ccggcggcgg cgcagcacga gcgcgagcac acccacacca agcagcagcg
     4561 ggccttgcgg cgtcgtgccc gcatcgcagc agccgctgtc gggagccggc caacacacgc
     4621 cggttccgcc ggacgcgagg cactcgaact cgttcgggca gctgcccgaa tttcccgggt
     4681 cgcacgtctc cgtgcagtgc ttgaacttct cgccaccgtc ggtgcagtgc tgcgagaggc
     4741 actcggcatc tgactggcat tgatcgccga ggccacctgg ctcgtcggga ccgggcaggc
     4801 agatgccgga gacgcagact tcgcttccgg tgcagccctt gctcgcggtg cacggcggcc
     4861 cttggttgac gctgatcgtc gtcgtcgcca tgacgtcttg cacgtcgtac gcacgcgctt
     4921 cgagcgtgtg tggacccatc gagaggtcgg tggggcccgt gatgtagaac ttgtcgtgga
     4981 actccgtcgt cgacatcccg atgagcatgc cgtcggcgta gagctcgacc ttggcgatgc
     5041 gaacgtcgtc gagcgctttg atgacggcgc gaaagccggg ctggatctcg cgaccttcgc
     5101 tcggcgacgt gaacgtgacc tcgggaggcg tcggtgcgcc cggcccgaac agattgagga
     5161 tcatccgata cgagttctgc ttcgcgcggc ctgcgcagtc acacgggcgc gtctcgagct
     5221 cgccgcaatc ggcgttgacg tcgcgaaacc gcttcggcag gtcaccgatg atgtacgtca
     5281 tcggatcttt ctgcaggaac tcgtgatcga ggccgaacgc gtgcgcgact tcctgcgccg
     5341 atacagcgca cagctgctcg gcatccgcgc cccacacgtc gaacgtaaag acgatcgcgt
     5401 ttggcacttc accgcacgtg aacggcgcga cgccgccggc gctcgacaga tcggtgcggc
     5461 cgagatccgt cggcttgcca ccgacgatgt tctcgaagtg aggcacgttg cccggatcga
     5521 cgtcggtcac ctgaatgttg aacggctcga acgtcttctt cacgcacgcg agctgcgcct
     5581 gccagacctc gtcggtctgc gtgaactcgc cgatgatgcg ttggccgttg acgatcgtcg
     5641 acgtatcggt gcgcgagtct tccgtcgacc cgaacttcag cgcgcagccg gcgccgatag
     5701 ggcagcgtcg caaatacacg agccgcgacg acacgacgtt cgacgggttc gagttgttct
     5761 ccgtcgggat gcggacccgc atcggcttgt agtgaccgct ctcgtaggtc tccaccttgc
     5821 gctccgcgga tgctgcgggc gcgagcgcgc agagaacgag accgatcctg attatctgcg
     5881 acgccgtcga catgtgtaga gacctaccgc aaacagaaga ccaagtgtca gcggaccgcc
     5941 ggggcgattc gactgacagc cacaaccccc agattcgtcg tagccgggcc agcaaaagcc
     6001 gttgccctgg ccgtcgtcga ggcagccaaa gccgctcggg cagtcgcccg gcgagcagtt
     6061 ctcgacgcaa tagagatgtt cgccgtcatt ggcgcactgg cccgactggc aatcggtggt
     6121 gaacatgcat gtcgcgccga gaccgcccgg ctcgtcgggg cccggcacgc agcgatcgcc
     6181 aacgcacgca tccgtcgcat tcgggcacgt cgtcgacgcg ttacacggcg ggttctgcac
     6241 gacgtccgtg ctcgccgtgg cgcgcgtctg ctgtgggtcc atcgccatcg cttcgatgtg
     6301 gtgcgggccg ttcgcgaggg aatccggcgc gtagaacacc cacggtgacg gcatcgagag
     6361 cgtgtcgatc atgctcgtgt cgatcgcggt gccatcgacc ttcagctcga gcttcgcgat
     6421 gaggttactg ttgtccatcg cctcgacctt gatcgcgaag cccttgtcga cggtggcgcc
     6481 ggccttcggg ctcgagatcg aaaccatcgg cggcgttcca gcgttcgtgc cgaacaggtt
     6541 cttgatgatc gcgaaggagt tctggctctg cgcttggccg ccggtgcacc tgcatgtatg
     6601 gctctgcgta tccgtgcccg tgcactggac gccgccagga ccgcgcccgg tcgctgcatc
     6661 gcagtcgctg ccgcactgtt ggttggcgtc ttggaagtac ttccgccggt tgtagttgaa
     6721 gtacgtcatc ggatctttct tatcgatgac gtgatccatg ttcgcccacg tgtggccgat
     6781 ttcctgcgcg gccgtcgtgc agatgttctc gatgcacgcg gtatcgcagt tcgtcgcgcc
     6841 gtagctccac gccttcgcga agtcgaacac gagcgcagtg ttgagatagc cgttgttgca
     6901 gcccggcgga gcgacgccgc cgatcatcgg atcgaagccg agatccgtcg gcagtccgcc
     6961 gatcatgatc tcgaagtgac ccgccgttcc cgggtccgtc tccgtgatca cgacgttgta
     7021 cggctcgaag acgtccttca cgcatgcgac gaccgcgttc caattcgcat ccgtgaacgg
     7081 aaacttcgta agcgtgcgct gcgctccgat cggccacgag ttgttgatcg aattcgactg
     7141 gccgacgttg atcacgcagc cgttcgcgca gcggttcaag tagagcttgt tcgtcgccgc
     7201 gagctccacg gtgccggtcg gcacgaacgt gggatcgtct ggccagtcga gcgtgccgtc
     7261 gcgcagccgc ggctcggcag cagccacctg agccgcgacg aggataaaga cgacaatccc
     7321 gaagcggcgc attgccatcc tgcgagccta cgctgccttg cggcggcgac gcgtgacaaa
     7381 agccgcaagg acaccaagtc ccagcagcat cggcccggcg ccgttcgagc ggccactggt
     7441 gtcgcagcag ccaccgtcgt cgaggcctgc ccagcacacg ccggtgccgt cggacttgcc
     7501 tgcgtcgagg cagccaaagc ccgtcgggca gctcgcatcg ttgttgaggt cgcacgtcgt
     7561 cgcgcacgcc atcatgttgc cgtcattggc gcacgcgccg tcgaggcagt cggcatccgt
     7621 cacgcacgtc gcgccgaggc caccggtgac ctgaggaccg gcgaggcacg ccttctcgta
     7681 gcagaagtag ccggcttcgc agccggcgtc gtcgacgcac gacggaccga acaggccgag
     7741 caggagcttc acgtcgtctt gcgtcgcctt gcccgtgctc atgcacgtgt gcgactgacc
     7801 gctgcacgtc aggccgaatg gcgactgacc attgacgcag tcactgccgc aggcgacgcc
     7861 gtccttgaac tgccggcgac cctggtactg gtagtacgtc atcgggtcgg tcttgtcggt
     7921 gacgtgatcg agcgtccacg tgtgcgcgat ctcttgcgcg gccgtcgcgc agatttcttc
     7981 gtcgcggttc gggatgccgt cgtacgagtt cgcgaagtca tagacgatcg tgttcggcag
     8041 gaacggcgtg cagacgccgg cctgctggca cgagtactcg gcgataccga gcacgccacc
     8101 cgagagcccg atctggccgg gcgttcccgc gatcatgatc tcgaagtggg gagcggtcat
     8161 gcccggatcg cgatcggtga tccggatgtt gaagcgcgag aacgtgtcct tcatgcacgc
     8221 gacgacggcg ttccacgagt catcgccgta cttgtacgcc gacagcgtgc cctgaccgat
     8281 ctgcgacttg ttggtccgcg agtccgtcgt gccgaacgtg atcttgcagc cgccggcgca
     8341 gcggttcagg aagatcgtcc gcgagttgac cgcgtgcgga acgtcttccg gcttcaccca
     8401 cgtcgggacg cgaacgaacc ggctgcggtc gagctccgcc tccgccgtcg acgcccaaga
     8461 caccaggcac acgacagcga ggacacccgc gatactgcgc atgaccacag atctatcccg
     8521 gtcggtggtc cgtcgcctag ctggaaaaag ccctagttgc gtgaggcctt agcggcggcg
     8581 acggcgaacg atcagaattc caacgagacc gaggctgaac agcaggaagc cttgaccgcc
     8641 gttgtgcgta acgccggcat cgcagccgcc gccgtcagac ggccagcaca ggccgttgcc
     8701 gccaccggtg tcgaggcagg cgaagccgct cgggcaggcg ctctgctcgg ggttgcacga
     8761 cgtcgtgcag tacgagtgat ctccgtcgtt gccgcactgg ccggtcgagc aatcttcgtt
     8821 gccgacgcaa tccgtgccga ggccgccttc catgctcgga cccgcgacgc agtgaccttc
     8881 gacgcagacg tcgccagccg tggtgcattc cgaatccgtc gtgcagacgg tgccgtactg
     8941 gacgttgacg gtcgcggtcg acttgttgcc cgcgtggtcg tagccgatga cttcgacctt
     9001 gtgcatgccc tgaccgaggg tcgcgggcgc tgcgctgttg aacggtgcgt cggtgagcgt
     9061 cttgatgacc ataccgtcga gcttgatctc gaccttctcg accgtgcggt cgtcgctgac
     9121 gtcggccttg atcgggaagc ccgcgttgac ctgcgccttg tccatcggga acgtgatgtt
     9181 gaccatcggc gcgacggtgt cgggtgcgct cgagccgaag accgactcga tcagctggtg
     9241 gctgttcatc ttcgccgtgc tgccgttgta ctggcactgg cagctacgcg cgctgtactc
     9301 gccgcacgag ccggccgcat cttggaagcg cttgtacgtc gggccacccg agagatacgt
     9361 catcgggtcg cggttgtcgt acttgtggtc gaggccccac gagtgagcag tctcctgcgc
     9421 gaccgtccag cagaggtcgg caacgttgct cggctcgagg ttcgcgaacg tgtacgagac
     9481 cgagttcgag atgtagccgc acgagaacgg cgagacgccg agcacgccgc tctgctgctg
     9541 cacttggttc ggcgtaccgg cgacgatcgc catgtggtag ttgccgctgg ccgggcgctc
     9601 ggtgacgatc tgaacgtcga agtccgcgta cgtctgcttg acgcactgga cgatcgcttg
     9661 ccagttcgcg tccgagccgg tgtacgccga cacggtcgac gacgcatcgg gaaccgacga
     9721 acggttgagc ggcgcgttgt ccgaaccggg gctcaactgg cagccgttcg gcttgcagtt
     9781 gttcatgtag atgacgtgcg agaccgcagc gctcgagtcc gggccgatcc attccttgcc
     9841 gagccggctc tcgtcgaccc acttcatggc ggtccgaggc gcggcggccg ccgttccgaa
     9901 catccccagg agcagagcgg ccgagcaggc aaaggtgcgg ttcatgggtt ggcgttgtag
     9961 ccgtcccggc agatctttcg aaagcacctc atgctgtcag tggctgaacc aacatgaggt
    10021 accgggtcgc gtggagtgcg acctagagat atcgctctag aaaatggccg cgcagacgat
    10081 gaaggccacg tacgcgagga tcaggatgac gccctctaac ctcgtgattt tacgggaccc
    10141 tctcagagtg agaacgccaa gcagcgtgat caccacgagg ccgatgagat cgaccagatg
    10201 catgcgctcg ccgaggtgga tcgggctgag ataggcggtc acgccgagca cgagaaacac
    10261 gttgagcaga ttcgagccaa tgacagatcc caccgcgagg gccgactgac ctcgggaggc
    10321 cgccaccaga gaaccgatga gctccgggag tgcgcagcca atcgagatga ccgtcagccc
    10381 gagcatgcga tcggacatgt ccagatcggc cgcgatgcgc cggcccccgc cgacgaacag
    10441 atacgagccg gcgaggagca gaacgaggcc gagcgtgctc atcaggatcg acatcagatg
    10501 gctcgcacgc gggcgtcctc tgccgccgat cgcgaggccg ctgtcttgtg cagtctgggc
    10561 gctcgacatc gatgccgacg aacccgcgtt ctccggctcg tcatcttcca tatcgagatc
    10621 gaggcgcgcc gaaacggaga gcgtgacgat cgtgaagacg atcgcgcagc cgatcagcaa
    10681 cgcgccttcg acccgcgaga gcacgccgtt gcgaaacagg atcggcacgg cgatcgccga
    10741 accgacgagc accggtacct cgcggcgaat gatgcggccg tcgacggtgg gtggcgcgat
    10801 caacgcggtg agcccgagga tgagcgagat gttggcggcg cacgatccga tgacggtgcc
    10861 gagcgcgatc ggttggctgt gcgcgagcgc ggtctttgtc gcgacggcga gctccggcgc
    10921 gctcgttcca tacgcgacga cagtgagccc gatgacgaga ggcttcacgc ccatcgcgcg
    10981 cgccatcgat gccgatccgc ggatcagcca ctcggcgccc caatagagaa ccgcgatccc
    11041 gaggatcagg agcgtgatgt cgaggacggt ggacaacccg ccgctagctt acttctgcgt
    11101 gcgtgcgagc tggcgggcgt cgtggatcaa ttcgtcgagc tggttgatcc gatccgagtc
    11161 gtagatgccc cgcaggtggg cttccttgtc gagcaggagg aagtagccct ggtgcgagat
    11221 atcgggcacg ccgttcggct tgtcgggcag ccgcatcatc gacgtcatga actgccgctc
    11281 gatcgtatcg tgcacggtgt cgaagtcgcc ggtaacgaag cgccagcggt ccggctgata
    11341 gcgatacttc gtcgcgtact cgaggaggcg ctgcggcgtg tcgtacttcg gatcgacgga
    11401 gaagctgacg agcttgatct tcttcgcgac gtcctcggtc ttggcttgga tgcgttccat
    11461 cttcatcgtg ctgaccgggc agatcgtgtc gcagcgcgtg aagatgaagt tgacgatcgt
    11521 gacgtggccg tgcagcgccg agtccgtgaa cgggcggttc atctcgtcga cgagcgagaa
    11581 ctggccgatc gttgcgaggt ccttcagttg cttgtccgcg ggccgcttca cgaagctcgg
    11641 gatgaagatc gtgagcgcga tcgcgacgac gatcccgagc agcacgagca gcagctttgg
    11701 gccggggatg cgttgcagcg cgtctcggtc gcgggtcacc ggcggaacct ggcagatccg
    11761 cgcacgaatt gcacgtagtc gcggctgagg cgtttcggcg cagggattgc tcctctagag
    11821 aggcgatgtg gctcgcccac gcgctgacgc tctcgcgaat cccgatcgcc gccgtgttct
    11881 ggctgactta cggccagagc cgcgcgctct cgctcgggct cgtcgtgctg gcagcgctca
    11941 ccgacgccgc cgacgggacc gtcgcccgct gggtgaaaca ccggggtggc tcgggctcga
    12001 cgctcggcga gtggctcgac ccggccgcgg acaagctgtt catcatcggc gtgctcggcg
    12061 cgattcaagt gcatgacccc gtgccgtggt cgttgatcgc gctgatcgtc gcgcgcgagc
    12121 tgctgttggt cccgctcgca gcgatctatc gcttcgtcgt ccacggacgc ggcgagcatg
    12181 cgttcaaggc aggcaacatc ggcaaggccg ccaccatcgc cgaaatcttt gcgatcggtg
    12241 cgatcattct gcactcgcgc gcggtcgtcc cgctggcggt cgccgccgca ggcctcggca
    12301 tcgccgcggt cgtctcgtac atctggcggg cgacgcacgt ccggcacgag gcagcgcacg
    12361 catgaagacg atgaagacgc acaagcgtgg cgacttcgag attctccacg ccacggcttc
    12421 gtcgcaagcc gcgatgatga cgttgccgcc caaaggtaag agcagcgagg gcagcctcaa
    12481 cgagcacgcg tgggccgagc agtggctcta cgtcgtgtcg ggcgaaggcc gcgtgacaag
    12541 ccgcaagaag aagtacccgc tcgagccggg cacgctcctg ctcatcgaga agggcgagcc
    12601 acacgagatc gagaacaccg gcacgacgcc gctcgtcacg ctcaacgtgt acgcgccgcc
    12661 cgcctacgac gacgatggcg agccgctata cggtgggacg gatccttagc gcgcgcgaac
    12721 gccggcgggc tgcttcgtgc cgacgcgcgt gaacacgagc tgcacgagct gcgtctcgcg
    12781 tgcgcggaag ccgccggcgc agccgagcag gtagtacttc cacatccgat agaacgtcgc
    12841 gccgtacttc gcctcgagct tgggccacgc cgcatcgaag cgctgccacc acgccatcag
    12901 cgtcggatcg tagtgttcgc cgatgttgtg cagatcctcg atgacgaaca ggcgctccat
    12961 cgcgttgatc attgcgtcgg gcgtcggcag caccgcgttc gggaagatgt atttgtcgaa
    13021 ccacggctcg atgtgcgtcg tcgtgcggtt gcccgtgatc gtgtgaacga agccgacgcc
    13081 tccgggcgcg agcagctgat tcacgagctc catgtacgcg cgatagttct tcggcccgac
    13141 gtgctccatc aggccgatcg agaccaccgc gtcgtacttg ccggtcgcct tgcgatagtc
    13201 gtcgtggcga atgtcgacgg gcaggtgggc gtacttctct ttcgcccagc gcacctgctc
    13261 ggcagcgacg gtgtagccgg tgacctcggc gccgtacttc tcggccgcat acgcggcgaa
    13321 cccaccccag ccgcagccga gatcgagcac gcgcatgccg gacttcaggc cgatcttctg
    13381 acagacgagc tcgagcttcg cttcctgcgc ggcttcgaga tcttttgcgt cgcgccaata
    13441 cgcgcacgtg tagagcatcc gcttgtcgag catcgcctcg tagaggtcgt tgccgatgtc
    13501 gtagtgcttg taggcgatct cgaacgcgcg ggtcttctgc ggattcagga cgcgcgcctt
    13561 gaccgcgtgc gcgaggagga cccagttgtc cttgacgtga tcgcgaaggc gatgacgaca
    13621 cacgcgatcg atcgtctggt cgagctcgcg cgaatcccac cagccctcca tgtacgcctc
    13681 gccaaagccg agcgtgccgt cgcgcagcag gcgctggtag aagcggtcgt ctttgacgtc
    13741 gatgtcccac ggatcgctgc cgccgacgac gatgccggcc tcggcgagcg cattgcggac
    13801 gagctcgttg gctcgacgcg cgtcgaactg agagagcagg gcgctagctg gcatgacgag
    13861 actccattcg atcgaggaca aaatcgatga ctttgggcgc gggcagtcgc gcaccggaga
    13921 acatccacgc aaagccgcga gccacggcgc gatcgaagcg gcctttcccg ccgccgttgc
    13981 cattcgactt cgccttgttc ttgaggcggc gtgcagcttc gacgacgcga tgcgtgcggc
    14041 gatagtcgcg gagcagcatg tccgagcgca gcgcctggac gtacgacgct ggatcgcccg
    14101 cgaggatcgc ttcggcggcg aggcgcccgg aggtgaccgc gggaacgatg ccttccgcgg
    14161 tgagcggatg cgcgaggccg agtgaatcgc cgacgagcag cgcggtgcca cggactgcac
    14221 cttcgagatg cgcggggtcg aacaagaagt acgagtgacc cttgaggtgc gcgagctgcg
    14281 gctctgcctc gtcgggaacg gtgcccttgt cgcggagcag cgagtgggtc gtcttccacg
    14341 cgtcgcggac cgccgtcgcg tcgagtgtgc cgcacccgat gttgagccaa tctttcttcg
    14401 gcacgttcca gccatagccg gagaggccgt cgaacagcac gagctcgggc tcgccatcct
    14461 tgccgaggcg cgtgcgcacg atggcatcgt gatcggtctg cagctccagc tcctgcacgc
    14521 cgaccgcacg acgcggacgc ggtggctcga gcaggcgagc gacagggcag tgcgtgccgc
    14581 cggcgccgac gagatacttc gcgcggagct caccgactcg ccacgtgccg tcgtcctcgc
    14641 gaacgatgtc cttcacgttg gttccgagat gcagctcggc gccgctctcg cgcaacagcc
    14701 agtcgtcgag ctcgacgcgg cggatgaacc agccgtggcc gttcagcgtg tactgcttgc
    14761 cgcgatagtg aacgtggcag ttgtgccact cccacagccc acgcgggtac tcgctcggcg
    14821 cgcgaccaat gacgtcccaa aacgtcggcg acagccaacc cgcgcagagc ttcacgcgcg
    14881 gaaactccgc tcgatcgatc accgcaaccc gcgcgcctct cgacgtcagc gtgcgcgcac
    14941 acgtgctgcc cgccggaccg ccgccaacaa tcaccgcgtc ataaagctcg gccacgacgg
    15001 cgatctacca cgggtttagc gctgaccggc gttcttcaac gcgtgccgcg cagaatgacg
    15061 ccgccgtcga gcgcgaggtg gatgtcgtcg gcagttgggc cccacgcgcc gtcgcagatt
    15121 tgcgtcgacg gcacgggcaa ctgctgctgc gtccacaccc catcgccttc cgacgtgatg
    15181 accgtattgc cgacgccgca cgcgagcacg aatccgtcga cggtaaagac gttgtagaga
    15241 tcgaacccga cgttcgcgtc ctgcgccgtc cacacgccgt tgccggttga gtgcagaatt
    15301 tttccggagt agccgacagc ccacacgtcg ttcgcgctcg aacccccgac tccgacgagc
    15361 ttcgcctgcg tccccgtgct ctgcggcgtc caggtcccat cgccggtcga gcgcagaagt
    15421 gtgccggcgt cgccgacgac gtagaagtcg ttaggtccgc tcgcccacca cgtgaagagc
    15481 gtcgcgctcg tgcccgtcga ttcggaagtc caactgccat cgccgttcga atgcgcgatc
    15541 agcccgccgt agccaatgac ccacatgttc gtcggcgatg cgacgtagcc gtcgttcaac
    15601 acctgcggca acgtgttcgg ttcctgctgc cagcctccgg ctccattcgg tcgcaagatc
    15661 gccatccccg aaggcgtcgc gttgccggcg acgatcacgt cgctcgccgt cgtcgcgccc
    15721 acgccataca gcgccgtcgt tacgggcacg ttctcgagcg tccaggtgtc attgcccgtc
    15781 gaatgcagca gcgcgctgcc attgccgacg gcatagatgt ccgtcgccga cgagccccag
    15841 atgtcgaaga gcttgtccgt cacgggcgtc gtctcggcgt gccacacgag cggcggtcgc
    15901 ggcggcttca catcgacggc cgcatcggga cgatcgccaa acgagaatcg accacagcct
    15961 gcaacgagca gcagtgcagc gatcctcatc cccgcagtct actgcgcgac gagcgcgcgc
    16021 gccttctcga tacccaggct cacagcacga tcgtgatctt cgatcggatc gacgacgcca
    16081 tccgtcccga caaacccgat gtacgcgacc gcctcgtcgg tgccgccggt gcgcggatcc
    16141 ggatcgatgc cgagcagctc cgccatccga tacgacgcct cgccgatgat cgccgtcggc
    16201 ccgacatccc ccgcaacgcc gtactgcacg ctgtccttat agacgaccgc aaacaccgag
    16261 cccatctgca ggcccgccgc gcgatagtcc cagcgagtgc tcttcccagg cacgacgaca
    16321 tacggcaacg ccgccgcatc gagcgcatcg ccgttcgaat ccgtcgcggc ggtctgattc
    16381 tgatacgccg gatccgtcga tagattgcac ttcgtcgtca tgacgccgtc gcaatcgaca
    16441 tccatctcgg agatccacca caccgcaccc ggcagcccgc acacgtccac cgtcgcggct
    16501 ccgcccgaat ccttcgcgta cggcccaccg atcacgttgc acgcaacgag cttgtcgcgg
    16561 agttgctgcg cggtcggcgg ctgccccata tccgcagcat ccggcgtcgc cgccgcatca
    16621 tccccgctac tacttccgca tgccgccatc gcccccgcga caacggccgc tactgctaga
    16681 cgtctgacca atcgttgtcc cgatcgatct gcgccgacga gatcgagttg agccacttcc
    16741 acgccgcgcg gcgccgctct ccatctttct cctcggccgc aaccaacgcc gacaaattgc
    16801 cgatggtcgg ttcgcgatcg atcacgatgc cgttgtgcat cgtctgagcg atgagatcag
    16861 cgagcgcagc cgggtcattc gcatgcacct ggatggcgat cgacatgcag cgggcgtaac
    16921 acaggtcgcc gccgcgtcgc cgctcaaatg cgcgctccgg agtgatccct acggcgcggc
    16981 atgtagcttc ggaaacgcgt cgcgatcatc gagccggacg aacgtgaagc cctgctgctc
    17041 gagcgctgtc agcacgtcat cgaggcgcgc cgccgtgttc gcgtggatgt cgtgcatcag
    17101 cagaatgccg ccgttgttcg ccttcgcctg cgcgacgaca aacccgacca tgttgtcccg
    17161 caacgcatcg tcgacatacc gaaatgtcga cctcttacag acgccacctc ccgccgcgta
    17221 acaccagtcc gcactgtcga tgtgccaccc ggtcacgacg tagcccttat tccgcgcgag
    17281 cttcatcccc gcgcacgtcg agctgccaaa cggaaaccga aagtacgccg gcgtctcgcc
    17341 cgcgccccga acgagcgcat ccgtcgcgtc gatctcgctg ccgactcgcg cgagcgtctg
    17401 ctgcgcaagg ttcaagtgat tctggctatg gttcgcaaga atgtaatcgg gatccgccgc
    17461 aatgcgcgcg gcaacctctt tcgcgccggc cacgctgtac cggctcccgt tgttgaagaa
    17521 cgccgccggc gcgccgtgcc gcttcaggac gtcgatcact ttcggcgtcg tcacgggatt
    17581 cggcccgtcg tcgaacgtca gagcgatgcg ctttccaaac ccgctccggt ccggaacgcg
    17641 gacgccggaa caatccgtgc cgtccgtctt cccctccgcc agcccgtcct cgtatgcctg
    17701 ctcgtagagc gcgctgtcct cgccctcgtc gtgctcgtcg atgctgtcgg cctcatcgag
    17761 acagccggtc gcggtcgcaa gtacacacgc gagcgaagcg aggatgcgca tggtgggctg
    17821 cccgtgagca acgaatacgc cgccccgcaa ttgtgcagca atgcgaagtt gcgcgcgcct
    17881 cgctggggtc agctgaccta cttgccggcg tcgcaagctt ccggtcgatg tgccgacgcg
    17941 cacgccgccc tcaccct
//
LOCUS       DATLBZ010000184        12858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3071382, whole genome shotgun sequence.
ACCESSION   DATLBZ010000184 DATLBZ010000000
VERSION     DATLBZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12858
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3071382"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VMZ53_09620"
     CDS             <1..684
                     /locus_tag="VMZ53_09620"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HUS28759.1"
                     /translation="AKDDPRRPKGSPMDPAVIASNHDPRQTWDPVTDWQLMGRNVRGF
                     MSLFYGDKPVVCKVHGFCVAGGTDMALCSDLLVIADDAKIGYPPARVWGSPTTSMWFY
                     RLGLEKAKRLLFTGDCLSGKEAKEWGLAIESAPPEQLNERFEILLERIARMPINQLVM
                     MKLLLNQTLMAQGLHQTQLLGTYFDGITRHTKEGYEFQRRAMEGGFKQAVRERDEPFG
                     DFGMSTFKG"
     gene            complement(681..1757)
                     /locus_tag="VMZ53_09625"
     CDS             complement(681..1757)
                     /locus_tag="VMZ53_09625"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HUS28760.1"
                     /translation="MALTIDDATRADLSRFGFDDAQLAIFAGRAGDTAQTYLTGTITP
                     PASEDVSLLPPVGSPAYNELATRGDVAIRAGEVGVVILAGGMATRFGGVVKAAVPVTH
                     GRSFLDLKLADLGGRAPAYVMTSFATHDRIRQQLVEENLSAELFPQLVSVRLTPNGEL
                     FREDDGALSPYATGHGDLPFALRARGVLKKFRDGGGKYLYMSNVDNLGATLEPAVIGA
                     HIARGAKVTAEVVRKEKGDKGGAPARVDGVPQIVEAFRFPPSFDQDTIPLFNTNTFVF
                     DAEAIDTDFNLTFFRVEKKVGNAKVIQFERLVGQLTAFLPSHFLEVPRSGPDGRFQPV
                     KDPEELELRRADIETILRAHNILR"
     gene            complement(1809..2483)
                     /locus_tag="VMZ53_09630"
     CDS             complement(1809..2483)
                     /locus_tag="VMZ53_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28761.1"
                     /translation="MRWLVVSVVVHAAVIAYLAYNSRDDETLVEWRLPTLPAYARVDT
                     VAVIELVDEPTVSGGGGGGGDEHQARHTTRTHARSPDAWEQIDLRMDAAGGSAGTGHG
                     TGDGGGNGNGNGHGIGLGTGGGVRVATDVPAPPPPPAISKARPAKLVWPTRDEEVDDD
                     ANLFTANVTVDAEGFVVGARMTATRPGPKAERAADAIWTFRYAPALDDDGRPVRSTFE
                     QSFQVR"
     gene            complement(2566..3144)
                     /locus_tag="VMZ53_09635"
     CDS             complement(2566..3144)
                     /locus_tag="VMZ53_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28762.1"
                     /translation="MLLLAGCSKGASSSCPSVEDLAQRAGSAAGRRELEIVVKRCIAD
                     DWPASVTKCLREAQNDAMIDPCFKALSPDQKARLEKAFAPINDEYAAKDRAEVRQFDV
                     LFGQRLAGLNIDQLPTRAPQCADYLAAIEAVRKATLDCKSDAIALEQYAIQQVIIADV
                     TKLAKTAEVDVGAACTQMAAGLRDEAKHICNH"
     gene            3193..4452
                     /locus_tag="VMZ53_09640"
     CDS             3193..4452
                     /locus_tag="VMZ53_09640"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HUS28763.1"
                     /translation="MRYALPLLTMLAISTAAGADDLHERATKLTRDAIIVDTHLDAPE
                     ALIEKWRDVAEKGATDHFDIPRAKAGGLTAPFFSIYVDPSYAEKGGAARALALIDLTR
                     RVIADHPSDMVAAASVDDIRAAKKAGKIGVLMGIEGGHAIENSLPVLREMYRAGVRYM
                     TLTHVNTNDWADSAGNFMIGEYDPKPFIKHHGLNDFGREVVKEMNRVGMIVDISHVSD
                     ETLEDVLEVSRAPVMASHSSCRALSSMPRNLSDDQIKRIAAKGGVVMINIGSQFLSQK
                     NWDAFVAMKKKLAPDVAKLKKKAGKDPDAFYSGLVALYAKNPPPRATLSDVADHIEHV
                     IKVAGIDAVGLGTDFDGIPDPAVGFDDYSKLPDLVAELLKRGHSDAEVKKILGENFLA
                     FFARVEAAKKSLASEKPSMATLKPAKK"
     gene            4547..7696
                     /locus_tag="VMZ53_09645"
     CDS             4547..7696
                     /locus_tag="VMZ53_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUS28764.1"
                     /translation="MWLIRTALRRPYTFVVMAVLLALGGVRAAQTTPTDIFPPINIPV
                     ISVLWNYGGLAPDQMEKRVTNGFERFLTTIVSDIDHVESQTLTGRTIIKIYMQPGADV
                     AQAIAQTTAVAQTAVRQMPPGAVPPLIMQYNATSVPIMMLALETDSLSEQQLFDYGIN
                     FVRSELTSIPGIQVPYPYGGKQRQVMVDIDPRRLHGVGLSPRDVQVALSEQNVILPTG
                     TVKLGNTEYPVLMASTPETLDELASFPVKTVNGRTIYIRDIANVRDGAMPQTNMVHVE
                     GKRSVLMVIMKNGDASTLDIADAVKAAIPRALDRLPKEARGHLTVKVMFDQSVFVRAS
                     ISGVIKEALIAAVLTGLMILLFLGSWRSTLTVITTIPLSIMFSVIVLQILGYTLNVMT
                     LGGLALAVGVLVDDATVAIENIHRNIGQRKPFVQAIVDGAQQIALPALVSTLCICIVF
                     LPIAFLTGAAKSLFVPLGLAVILAMLMSYVLSRTLLPTMVHYLLRREAAHGHKPNAFT
                     QRFDAAFDRLRHGYGRWLAWTLKNRAFTVVLFLAFVGGTLYMTPMLGRDFFPQVDAGL
                     IKLHVRTTPGTRIEETEKRIAAIERTIRTVIPKNEVDSMIDIVGTPYSSINLSLSEGA
                     LISSADAQIMISLKEGHRPTADYLRTLRTHLAKTYPDTEFFFLAPDISTQVLNFGLAA
                     PIDVQVVGPIGSEDKTQAYAQQIAARIEKIKGAADVHMAQVNKVPNLQISVDRVMAQQ
                     AGLSEREVASDLLVSLASSGQTAPTYWIDKRGVQYTVSVQTPQYEIDSIDELKATPIS
                     TGGAPQTLGNLATIKRGEGPENITHDDIKRTYDIQANVADRDLGGVTDDVRAVIAQMA
                     KQAPPGVKVTLKGQAQSMDSSFAGLRFGLVFAILLVYLLMVVFFQSWLDPFIILMALP
                     GALAGIIWILFLTGTTLSVPALVGAIMCVGVATANSILVVSFANEQRRIGRDERSAAL
                     AAGMTRLRPVMMTALAMIIGMLPMAIGFGEGGEQNAPLGRAVIGGLGLATLTTLFFVP
                     VMYSLLRRKPPRNPEEVLP"
     gene            7693..8958
                     /locus_tag="VMZ53_09650"
     CDS             7693..8958
                     /locus_tag="VMZ53_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUS28765.1"
                     /translation="MTEQSPDDLGFELPPPAQSSWLKILVIAVIVVGVVFAIGYMNHQ
                     KARGGTPITATEMGTLRVEVFNPKEHSSENALALPGVVRPLEEAKIYSRSQGYVRKWL
                     VDIGDKVKQDQLLAEIDTPELDAQLAQARAQLASARASVKQASAQRDFAKQSSTRYES
                     LADQKLVSKAAVEQQQAEAATGDANVAAAQSNVAAQEANVRRLTELQGYSKVTAPFAG
                     TITARTIDRGTLVGEGGATPMFTLVATDPVRIFVDVPQSVAPSIRVGTDAAVTVREFA
                     GKSFAGKVTRSSGSLDPELHTMSTEIQVPNPESQLLPGMYVQVQLTLPVPHRVLEIPA
                     TALYSDAQGTRVATVDAQNKIKMVPITIERDTGSTLWIGSGLTGTERVVKIAIPSALD
                     GDPVEVVQPPAPPSAGSGSATVSAQKRSP"
     gene            complement(8940..9560)
                     /locus_tag="VMZ53_09655"
     CDS             complement(8940..9560)
                     /locus_tag="VMZ53_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HUS28766.1"
                     /translation="MRAELHYTLTPAGRFNIAPGQSAPVGFTRDGTRRIDALRWGLLP
                     RWRGHGGKRGAMVCSAPLEAAAGTPQLRDAFKKQRCLVLADGCYAWRELKQPIWYHPE
                     PRHVVAFAGVWEESEDDGVASFALLLGPPLVTRVRDAMPIVLAPKDYDAWLDPKVKPE
                     DVMDIVVASQLVGWRADAVSTRMSQAQYDDERCIEPVGNPNQGDLF"
     gene            9632..9883
                     /locus_tag="VMZ53_09660"
     CDS             9632..9883
                     /locus_tag="VMZ53_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28767.1"
                     /translation="MERFPRGSIPPSAFAAVATMAPTAIAKVPAAVAASTEPPTEAVI
                     CPTKACDLSTTCPSCGQQMQPEHAHYRCGGCGYRDSCCF"
     gene            10069..10677
                     /locus_tag="VMZ53_09665"
     CDS             10069..10677
                     /locus_tag="VMZ53_09665"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HUS28768.1"
                     /translation="MTLDRALAAIDAAAESKVDAIKFARMPWAWSSKLIQRAEGRSVK
                     MLVTALDEDAITRFDWLGASAFYLVFDWSDLDLVARAAQTGKPVVMQVGTASEVELAE
                     VVATVHANGTGGIALVQSVIDVDLEGLEGMRCHGSVVGIADRSFGPVIPMAAVSKGAA
                     IVEKRFALALNGALSPAEMGPVVRDIEQAWASLGDDRRWTVN"
     gene            10702..10953
                     /locus_tag="VMZ53_09670"
     CDS             10702..10953
                     /locus_tag="VMZ53_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28769.1"
                     /translation="MNHPKMTSSGRRWAFLIGLTVAFALPKNVECGYPNGQCTEIIDR
                     QECARYEVEPWGFSLLERVFKRDIGFAYKTGIACGGDRL"
     gene            11004..12137
                     /locus_tag="VMZ53_09675"
     CDS             11004..12137
                     /locus_tag="VMZ53_09675"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUS28770.1"
                     /translation="MSKLTSFPGGLLLGASLMYFLDPIRGRGRRRRIQEAVHHAERVE
                     RDLFAKAAKDTRHRIHGLVERVSHPPATDVGDEVIEQRLRARLGRALSHPRALDIVVV
                     AGRVILRGPILTHEASDVIKLARAVPGVREVIDRLERHTSAGSVPALQGQGRRRKAKG
                     TWPPAAQAGATGAGALMLAYGLLLRRGIVGTLVGTAGGALALRGSVNKPFQELVGARA
                     APVTVHKSIVVHQPIHIVFDCWSRFDNFPMFMQHVREVDLQVGGSHSRWTVDGPAGTK
                     VEFEAETTDFEPDRTIAWKTIANQPIEHEGRVRFEDVEGGTRVTVTMSYLPPGGIIGH
                     AVAHILGWDPKARMDEDLVRMKTLLEEGKTRAHQQSVELADLH"
     gene            12147..12695
                     /locus_tag="VMZ53_09680"
     CDS             12147..12695
                     /locus_tag="VMZ53_09680"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HUS28771.1"
                     /translation="MPRPRDVIAKRPRILFVGINPGATSGKVGHHFAGAGNPFWKLLH
                     AAGLTPVELTAAEDGRLPEFGHALVNLCARPTKTAAELSRKELQRGRELLLEKIREMK
                     PRVVALVGITLYPLVLGLKGKLAHPGPGAKPETLEGARVFVVPNPSGLNASFPTFAAK
                     LPWFEALGDYAGMSGQPSPRSK"
     gene            complement(12653..>12858)
                     /locus_tag="VMZ53_09685"
     CDS             complement(12653..>12858)
                     /locus_tag="VMZ53_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28772.1"
                     /translation="RCVHPAGPPVCWCRKPMPGLALAFARAHDVDLARSVHVGHGPAD
                     KGFAIRAGVDYFDRGDGWPDIPA"
BASE COUNT         2301 a         4275 c         4099 g         2183 t
ORIGIN      
        1 gcgaaagacg atccgcgacg gccgaaaggc tcgccgatgg atccggcggt gatcgcgagc
       61 aatcacgatc cgcgacagac gtgggacccg gtcaccgact ggcagctgat gggccgcaac
      121 gtccgtggct tcatgagcct gttctacgga gacaagccgg tcgtctgcaa agtgcacggc
      181 ttctgcgtcg cgggcggcac cgacatggcg ctgtgctccg acctgctcgt gatcgccgac
      241 gacgcgaaga tcggctaccc gccggcgcgc gtgtggggat caccgacgac gtcgatgtgg
      301 ttctaccgcc tcggcctcga gaaggcgaag cgcttgctgt tcaccggcga ctgcctgtcc
      361 ggcaaagaag cgaaggagtg gggcctcgcg atcgagtcgg cgccacccga gcagctgaac
      421 gagcgcttcg agatcctgct cgagcggatc gcgcgcatgc cgatcaatca actcgtcatg
      481 atgaagctgc tgctcaacca gacgctcatg gcgcagggcc tccaccagac gcagctgctc
      541 ggcacgtact tcgatggcat cacgcgccac acgaaggaag gctacgagtt ccagcgtcgc
      601 gcgatggaag gcggcttcaa gcaagcggtg cgcgaacgcg acgagccgtt cggcgacttc
      661 ggcatgtcga cgttcaaggg ttagcgcaga atgttgtgcg cgcgcaggat cgtctcgatg
      721 tccgcgcgcc gcagctcgag ctcttcggga tccttcaccg gctggaaccg tccgtcggga
      781 ccgctgcgcg gcacctcgag gaagtggctc ggcaggaacg cggtcagctg accgaccagc
      841 cgctcgaact ggatcacctt cgcgttgccg actttcttct cgacgcggaa gaacgtcagg
      901 ttgaagtcgg tgtcgatggc ttctgcgtcg aacacgaacg tgttcgtgtt gaacagcggg
      961 atcgtgtcct ggtcgaacga cggtgggaat cgaaatgcct cgacgatctg cggcacgccg
     1021 tcgacgcgcg cgggcgctcc gcccttgtcg cctttctctt tgcgcacgac ctccgcggtg
     1081 accttcgcgc cacgcgcgat atgcgcgccg atcacggcag gctcgagagt cgcgcccagg
     1141 ttgtcgacgt tcgacatgta gagatacttg ccgccgccat cgcgaaactt cttcaagacg
     1201 cccctcgcac gcagcgcgaa cggtagatcg ccgtgcccgg tcgcatacgg cgacagcgcc
     1261 ccatcgtcct cgcgaaacag ctcgccgttc ggcgtgagcc gcacgctcac cagctgcgga
     1321 aagagctccg cgctgaggtt ctcctcgacg agctgctggc ggatccgatc gtgtgtcgca
     1381 aagctcgtca tcacgtacgc cggcgcccgc ccgccgagat ccgcgagctt gagatccaag
     1441 aacgaccggc catgcgtcac cggcaccgcg gccttcacga cgccgccgaa tcgcgtcgcc
     1501 atgccgcccg caagaatcac gacgccgacc tcgcctgcgc gaatcgcgac atctcctcgc
     1561 gtcgcgagct cgttatacgc aggcgagccc accggcggga gcaggctcac gtcctcgctc
     1621 gcaggcggcg tgatcgtccc ggtcagatac gtctgcgcgg tgtcgccagc gcggcccgcg
     1681 aatattgcta gctgcgcatc gtcgaaaccg aagcgcgaga ggtcggcacg cgtcgcgtcg
     1741 tcgatcgtca aagccatgcg cggagcatat gcgcccgctc gggagcagtt agccaacctg
     1801 actggaatct agcggacctg aaacgactgc tcgaacgtgc tccgcaccgg ccgtccgtcg
     1861 tcgtcgagcg caggcgcata gcgaaacgtc cagatcgcat ccgccgcacg ctccgctttc
     1921 ggtccgggcc gcgtagcggt catgcgcgcg ccgacgacaa agccctcggc atcgacggtg
     1981 acgttcgccg taaacaggtt cgcatcgtcg tcgacctcct cgtcgcgcgt cggccacacg
     2041 agcttcgcgg gcctcgcctt cgagatcgcg ggcggtggcg gtggcgcagg cacatcagtc
     2101 gcgactcgca cgccgcctcc agtgccaagg ccgatgccgt gaccgttgcc attgccgttg
     2161 ccgccgccgt cgccggttcc atgtcccgtt ccagcgctcc cgccggctgc gtccattcgc
     2221 aggtcgatct gttcccacgc atccggcgag cgagcgtggg ttcgcgtcgt atggcgcgcc
     2281 tgatgctcgt ctccaccacc gcctccaccg ccgctcacag tgggctcgtc cacgagctcg
     2341 atcaccgcga ccgtatcgac tcgcgcgtag gccgggagcg ttggcaggcg ccactcgaca
     2401 agcgtctcgt cgtcgcgcga gttgtaagcg aggtaggcga tcaccgccgc gtgaacgacg
     2461 acagagacga cgagccaacg cacgtctcga tcatgcctcg gcgttgcggc gaatccagct
     2521 cgctaatgct gagcgcgtga gaagtgcggg ccgcgctgcg acacctcagt gattgcagat
     2581 gtgcttcgct tcgtcgcgca ggcctgccgc catttgcgta cacgctgcac caacgtcgac
     2641 ctccgccgtc ttcgcgagct tcgtgacgtc cgcaatgatc acttgttgga tcgcatactg
     2701 ctcgagcgcg atcgcgtcgc tcttgcagtc gagtgtcgct ttgcgcacag cctcgatcgc
     2761 agcgaggtag tccgcgcact gcggcgcgcg cgttggcagc tggtcgatgt tcagcccggc
     2821 cagcctctgg ccgaacaaca cgtcgaactg ccgcacttct gcgcggtctt tggcggcata
     2881 ttcgtcgttg atcggcgcga acgctttttc caggcgcgcc ttctggtcgg gcgatagcgc
     2941 cttgaagcac ggatcgatca tggcgtcgtt ctgtgcctcg cggaggcact tcgtcacaga
     3001 cgcgggccag tcgtcggcga tgcagcgctt cacaacgatc tcgagctcgc ggcgaccggc
     3061 agcggagccg gcgcgctggg caaggtcttc gaccgatggg cacgatgacg acgcaccctt
     3121 gctgcaacca gccagcaaaa gcaccaaggc gagggtacgc actcgcgccc tatagcagat
     3181 aacctccggg cgatgcgata tgcgcttcct ctcctaacga tgctcgcgat ctcgacggcg
     3241 gccggcgccg acgatctgca cgagcgcgcg acgaagctca cgcgcgacgc gatcatcgtc
     3301 gatacgcacc tcgacgcacc cgaggcactg atcgagaagt ggcgcgatgt ggcagagaag
     3361 ggcgcgaccg accacttcga cattccgcgc gcgaaagccg gcgggctcac cgccccgttc
     3421 ttctcgatct acgtcgatcc gagctacgcc gaaaagggcg gtgcggcgcg cgcgctcgcg
     3481 ctgatcgacc tcactcgtcg cgtcatcgcg gatcacccaa gcgacatggt cgccgccgcc
     3541 tcggtcgacg acattcgcgc cgcgaagaag gccggcaaga tcggcgtgct catgggcatc
     3601 gagggcgggc acgcgatcga gaattcgctt ccggtgctgc gcgagatgta tcgcgccggt
     3661 gtgcgctaca tgaccctgac gcacgtcaac acgaacgact gggccgactc cgccggcaac
     3721 ttcatgatcg gcgagtacga tccgaagcca ttcatcaagc accacgggct caatgacttc
     3781 ggccgcgagg tcgtgaagga gatgaaccgc gtcggcatga tcgtcgacat ctcgcacgtg
     3841 tccgacgaaa cgctcgaaga tgtgctcgag gtatcgcgcg cgccggtgat ggcctcgcac
     3901 tcgtcgtgcc gcgcgctctc gagcatgccg cgcaacctca gcgatgatca gatcaagcgg
     3961 atcgctgcga agggtggcgt cgtgatgatc aacatcggct cacaattctt gtcgcaaaag
     4021 aattgggatg ccttcgtcgc gatgaagaag aagctcgcgc ccgacgttgc gaaattgaag
     4081 aagaaggccg gcaaggaccc ggacgcgttc tatagcgggc tcgtcgcgct gtacgcgaag
     4141 aatccgccgc cgcgggcaac gctgagcgat gtcgccgatc acatcgagca tgtcatcaag
     4201 gtcgcgggca tcgatgcggt cgggctcggt acggatttcg acggcatacc cgacccggcc
     4261 gttggctttg acgactactc gaagctgccc gacctcgtcg ccgagctcct gaagcgcggc
     4321 cacagcgatg cggaggtgaa gaagatcctg ggcgagaact tcctcgcgtt cttcgcgcgt
     4381 gtcgaggcgg cgaagaagtc gctcgcttcc gagaagccga gcatggcgac gctcaaaccc
     4441 gcgaagaagt aggacaactt cacgcgatgc cgggacccgc cctacgtaac gaactgttgc
     4501 gagcctagac agcggcgtgg ggaggggcac actgggggcg ctatccatgt ggctgatccg
     4561 taccgccctt cgcaggccgt acacgtttgt cgtgatggcg gtgttgctcg cgctcggtgg
     4621 cgtgcgagcg gcgcagacga cgccgaccga catcttcccg ccgatcaaca ttccggtgat
     4681 ctccgtcctc tggaactacg gcggtctcgc acccgatcag atggagaagc gcgtcacgaa
     4741 cggcttcgag cgattcctga cgacgatcgt cagtgacatc gaccacgtcg agagtcagac
     4801 gctcacgggc cgcacgatca tcaagatcta catgcagccc ggtgcggacg tcgcgcaggc
     4861 gatcgcacag acgacggctg tcgcgcagac cgccgtgcgc cagatgccgc cgggggccgt
     4921 gccgccgctc atcatgcaat acaacgccac gagcgtgccg atcatgatgc tcgcgctcga
     4981 gacggactcg ctctccgagc agcagctgtt cgattacggc atcaactttg ttcgctcgga
     5041 gctcacgtcc atcccgggga tccaggtgcc gtatccgtac ggcggcaagc agcgtcaggt
     5101 catggtcgac atcgatccgc gccggctgca tggcgttggg ctctcgccgc gtgacgtgca
     5161 ggtcgcgctc tccgagcaga acgtgatcct gcctaccggc acggtgaagc tcggcaatac
     5221 cgagtatccg gtgctcatgg catcgacacc ggagacgctc gacgagctcg cgagcttccc
     5281 ggtgaagacc gtgaacggcc gcacgatcta catccgcgac atcgcgaacg tgcgcgacgg
     5341 tgcgatgcct cagacgaaca tggtgcacgt cgagggcaag cgctccgtgc tcatggtcat
     5401 catgaagaac ggcgacgcct cgacgctcga tatcgccgat gccgtgaagg ccgcgatccc
     5461 gcgtgcgctc gaccggctgc cgaaggaagc gcgcggccac ctcaccgtga aggtgatgtt
     5521 cgatcagtcc gtcttcgtgc gggcgtcgat cagcggcgtc atcaaagagg cgctcatcgc
     5581 ggcggtgctg acggggctca tgatcctgct gttcctcggc agctggcgct cgacgctgac
     5641 ggtcatcacg acgatcccgc tgtcgatcat gttctccgtg atcgtgctgc agatcctcgg
     5701 ctacacgctc aacgtgatga cgctcggcgg gctcgcgctc gcggtcggcg tgctcgtcga
     5761 cgatgcgacg gtcgcgatcg agaacatcca tcgcaacatc ggccagcgaa aaccgttcgt
     5821 gcaggcgatc gtcgacggtg cgcagcagat cgcgttgccc gcgctcgtgt cgacgctctg
     5881 catctgcatc gtgttcttgc cgatcgcgtt tctcaccggc gcagcaaagt cgctgttcgt
     5941 gccgctcggc ctcgcggtca tcctcgcgat gctgatgtcg tacgtgcttt ctcgaacgct
     6001 gttgccgacg atggtccact atctactgcg tcgagaagct gcgcatgggc acaagccgaa
     6061 cgcgttcacg cagcgcttcg atgcggcgtt cgatcggctt cgccatgggt acggccgctg
     6121 gctcgcgtgg acgctgaaga accgcgcatt caccgtcgtg ctgttcctcg cgttcgtcgg
     6181 cggcacgctc tacatgacgc caatgctggg ccgcgacttt ttcccgcagg tcgacgctgg
     6241 cctcatcaag ctgcacgtgc gaaccacgcc cggcactcgc atcgaggaga cggagaagcg
     6301 catcgctgcg atcgagcgca cgattcgcac cgtgatcccc aagaacgaag tcgactcgat
     6361 gatcgacatc gtcggcacgc cgtactcgag catcaactta tcgctgagcg aaggcgcgct
     6421 catctcgtcg gccgatgcgc agatcatgat ctcgctcaaa gagggccatc gaccgaccgc
     6481 ggactacctg cgcacgttgc gcacacatct cgcgaagacg taccccgata ccgagttctt
     6541 cttcctcgcg ccggatattt cgacgcaggt cttgaacttc ggtctcgccg ccccgatcga
     6601 cgtgcaggtc gtcggcccca ttggcagcga ggacaagacc caggcgtacg cgcagcagat
     6661 cgccgcgcgc atcgagaaga tcaaaggcgc cgccgacgtg cacatggcgc aggtcaacaa
     6721 ggtgccgaac ctccagatct ccgtcgaccg cgtgatggcg cagcaagccg gcctctccga
     6781 gcgcgaggtc gcgagtgacc tgctcgtctc gctcgcatcg agcggccaga cggcaccgac
     6841 gtattggatc gacaagcgcg gcgttcaata caccgtcagc gtgcagacac cgcagtacga
     6901 aatcgattcg atcgacgagc tgaaggccac gccgatctcg acgggcggcg cgccgcagac
     6961 gctcggcaac ctcgccacga tcaagcgcgg cgaaggtccg gagaacatca cgcacgacga
     7021 catcaagcgc acgtacgaca tccaagcgaa cgtcgccgat cgcgatctag gcggcgtcac
     7081 cgacgacgtt cgtgctgtca tcgcgcagat ggcgaagcag gcgccacccg gcgtgaaggt
     7141 cacgctgaag gggcaagcgc aatcgatgga cagctcgttc gccggcctgc gcttcggcct
     7201 cgtgttcgcg atcctgctcg tctatctgct gatggtcgtg ttcttccagt cgtggctcga
     7261 cccgttcatc attctgatgg cgcttcctgg tgcgctcgcg ggcatcatct ggattctgtt
     7321 tctcaccggg acgacgctct cggtgcctgc gctcgtcggc gcgatcatgt gcgtcggtgt
     7381 cgcgaccgcg aactcgatcc ttgtcgtgtc gtttgcgaac gagcaacggc gcatcggccg
     7441 tgacgaacgc agtgccgctc ttgccgccgg catgacacgc ttgagaccgg tcatgatgac
     7501 agcgctcgcg atgatcatcg gtatgttgcc gatggcgatc ggatttggcg aaggcggcga
     7561 gcagaacgcg ccgctcggcc gcgcggtcat cggtggtctc gggctcgcga cactcacgac
     7621 gctgttcttt gttcccgtga tgtacagctt gctgcgtcgc aagccgccgc ggaatcccga
     7681 ggaagtactc ccatgacaga gcagtcccca gatgatctcg gcttcgagct gccgcctccg
     7741 gcgcagagct cgtggctcaa gatcctcgtg atcgcggtga tcgtcgtcgg tgtcgtgttc
     7801 gcgatcggtt acatgaatca ccagaaggcc cgcggcggca cgccgatcac ggcgaccgag
     7861 atggggacgc tgcgcgtgga ggtgttcaat ccgaaggagc actcgagcga aaacgcgctc
     7921 gcgctgcctg gcgtcgtgcg gccgctcgaa gaagcgaaga tctactcgcg cagccaaggc
     7981 tacgtgcgca agtggctcgt cgatatcggc gacaaggtga aacaagacca gctgctcgcc
     8041 gagatcgata cgcccgagct cgatgcccag ctcgcacagg cacgcgctca gctcgcatct
     8101 gctcgcgcga gcgtcaagca agcctcggcg cagcgcgact tcgcgaagca gagctcgact
     8161 cgctacgagt cgctcgccga tcagaagctc gtgtcgaagg cagccgtcga gcagcagcag
     8221 gcggaggcag cgacgggtga cgccaacgtc gcggccgccc agagcaacgt cgccgcgcaa
     8281 gaagcgaacg tgcgccggct gacggagctg cagggctact cgaaggtcac agctccattc
     8341 gcggggacca tcacggcgcg cacgatcgat cgcggcacgc tcgtcggcga aggcggcgcc
     8401 acgccgatgt tcacgctggt cgcgacagat cccgtccgca tcttcgtcga cgttccccag
     8461 agtgttgcgc cgagcattcg cgtcggcacc gatgcggccg tgacggttcg cgagttcgcg
     8521 ggcaagagct tcgcgggcaa ggtcacgcgc tcgtcgggct cgctcgatcc cgagctgcat
     8581 acgatgtcga cggagatcca ggtgccgaac cccgagagcc agctactgcc cggcatgtac
     8641 gtgcaggttc agctcacact gcccgtcccg catcgcgtac tcgagattcc agcgaccgcg
     8701 ctctacagcg atgcgcaagg cacgcgtgtc gcgaccgtcg acgcgcagaa caagatcaag
     8761 atggtgccga tcacgatcga gcgcgacacc ggctcgacgc tgtggatcgg cagcggcctc
     8821 accggcacgg agcgcgtcgt gaagatcgcg attccgtcgg cgctcgacgg cgatccggtc
     8881 gaagtcgtgc agccacccgc gccgccgtcc gcggggtcag gctcggcaac ggtgagcgct
     8941 cagaagaggt cgccctgatt cggattgccg accggctcga tgcagcgttc gtcgtcgtac
     9001 tgcgcttggc tcatccgcgt cgacacggcg tctgcgcgcc agccgacgag ctgtgacgcg
     9061 acgacgatgt ccatgacgtc ttcgggcttc accttcggat cgagccaggc gtcgtagtcc
     9121 ttcggcgcta gaacgatcgg catcgcgtcg cgcacgcgcg tgacgagcgg cggtccgagc
     9181 agcagcgcga agctcgcgac accatcgtct tcactctctt cccacacgcc tgcgaacgcg
     9241 acgacgtgac gcggctcggg gtggtaccag atcggctgct tgagctcgcg ccacgcatag
     9301 caaccatccg cgagcacgag gcagcgctgt ttcttgaacg catcgcgcag ctgcggtgta
     9361 cctgcggcgg cttcgagcgg cgcagagcaa accatcgcgc cgcgcttgcc cccgtgaccg
     9421 cgccagcgcg gaagcaggcc ccagcgcagc gcatcgatcc ggcgcgtgcc gtcgcgcgtg
     9481 aacccgacgg gcgcactctg ccccggggcg atattgaatc gacccgctgg ggtgagcgtg
     9541 tagtgcagct ccgcgcgcat gccctctccc aaggctagcg ccaccgacgg gagcgccaag
     9601 tgtgtcagac cgatctgata tggacggcat catggagcga ttcccacgag gctcgatccc
     9661 gccctcagcg tttgcggcgg tcgcgacgat ggcgcccact gcaatcgcaa aagtccccgc
     9721 agccgttgcc gcgtcgacgg agccgcctac cgaggcagtc atctgcccga cgaaggcgtg
     9781 cgatctctcg acgacctgcc cgagctgcgg ccagcagatg cagcccgagc acgcgcatta
     9841 ccgctgcggc ggatgcggat atcgcgattc ttgctgcttc taagcgagta gaacccctgc
     9901 gagattttcc gagcgcattt caggaaggtg cgtttcacgg tttcccgccc tctttttcgg
     9961 tcaagcttgg tcgagttacg gggggctatg agagcaatcg acctgggtag gccggtgggc
    10021 cgcgcacatc caccattcat tattgctgcg ctcgattgtc gggagctggt gactctcgac
    10081 cgcgcgctgg cagcgatcga tgcggcggcg gagagcaaag tcgacgcgat caaattcgcg
    10141 cgcatgccgt gggcatggag ctcgaagctg atccaacgcg cagaagggcg cagcgtgaag
    10201 atgctggtca cagcgctcga cgaagacgcg atcacgcgct tcgactggct cggcgcatcg
    10261 gcgttctatc tcgtattcga ttggtcggat ctagatctcg tggcgcgggc tgctcagacc
    10321 ggtaagccgg tcgtgatgca ggtggggacc gcgtccgagg tcgagcttgc cgaggtcgtc
    10381 gccaccgttc acgcgaacgg aaccggcggc atcgcgctcg tccaatcggt gatcgatgtc
    10441 gatctcgaag gcctcgaagg gatgcgctgt catggctcgg tcgtcggcat cgcggaccgc
    10501 tcgttcggtc cggtgattcc gatggcggcc gtctccaaag gtgccgcgat cgtcgagaag
    10561 cggttcgcgc tcgcgctcaa tggtgcgctg tcgcccgccg aaatgggtcc ggtcgttcgc
    10621 gacatcgagc aggcgtgggc atcgctcggt gacgatcgtc gctggaccgt caactaagtc
    10681 caactaggtt acgctcggcg cgtgaaccat ccgaagatga cgtcgagcgg gcgccggtgg
    10741 gcgttcctga tcggcctcac cgtcgcgttc gcgctgccaa agaacgtcga gtgcggatat
    10801 ccgaacggcc agtgcaccga gatcatcgat cgtcaggagt gcgcgcgtta cgaagtcgag
    10861 ccgtggggct tctcgctgct cgagcgtgtg ttcaagcgcg acatcggctt cgcgtacaag
    10921 accggcatcg cctgcggcgg cgatcgcctc taaggatttc tccggctggc gcatgtcttg
    10981 ccatcacatc gatgggaggc gacatgtcga agctaacgag ttttcccggc ggcctactcc
    11041 tcggtgcgag cctcatgtat ttcctcgatc cgatccgcgg ccgagggcgc agacgccgca
    11101 tccaagaggc cgtgcaccac gcagaacgtg tcgagcgcga cctgttcgcg aaggccgcga
    11161 aagacacgcg acatcgcatc cacggcctcg tcgagcgtgt cagccatcca cctgcgacgg
    11221 acgtcggcga cgaagtgatc gagcagcgac tgcgcgcacg gttaggccgc gccctgtctc
    11281 atccgcgcgc gctggacatc gtcgtcgttg caggtcgcgt catcctgcgc ggcccgatcc
    11341 tcacgcacga agcaagcgac gtcatcaagc tcgcgcgcgc ggtccccggc gtccgcgaag
    11401 tgatcgaccg gctcgagcga catacgtcag ccggaagtgt tcccgcgctg caagggcagg
    11461 gacgacgccg caaagcgaaa ggcacatggc cacccgcggc ccaagcaggt gccaccggcg
    11521 caggcgcatt gatgcttgcc tacgggctgc tcctgcggcg cggcatcgtc ggcacgctcg
    11581 tcggaacagc aggaggtgca ctcgcgctgc gcggctcggt gaacaagcca ttccaagagc
    11641 tcgtcggtgc gcgcgccgcg ccggtgaccg tccacaaatc gatcgtcgtc caccagccga
    11701 tccacatcgt gttcgattgc tggtcgcgct tcgacaactt cccgatgttc atgcagcacg
    11761 tgcgggaggt cgatctgcaa gtcggcggct cgcactcgcg ctggaccgtc gacggccccg
    11821 caggcacgaa agtcgaattc gaagcagaga cgaccgactt cgagcccgat cgtacgatcg
    11881 cgtggaagac gatcgcgaac caaccgatcg agcacgaagg ccgcgttcgc ttcgaggacg
    11941 tagagggcgg cacccgagtg accgtgacga tgtcgtatct cccgccaggc ggcatcatcg
    12001 gccacgccgt cgcgcacatc ctcggttggg atcccaaagc ccgcatggac gaggacctcg
    12061 tccgcatgaa gacgctgctc gaagaaggca agacgcgcgc gcaccagcag tcggtcgagc
    12121 tggccgacct gcactaagat cgctgcgtgc cgcgcccgcg tgacgtcatc gccaaacgcc
    12181 cgcggatcct gttcgtcggg atcaacccag gcgcgacgtc gggcaaggtc ggccaccact
    12241 tcgcgggcgc gggcaatcca ttctggaagc tcctccacgc cgctggcctc acgccggtcg
    12301 agctcactgc agctgaagac ggccgcctcc ccgaattcgg ccacgcgctc gtcaatctgt
    12361 gcgcgcgccc gaccaagacc gcggccgagc tgtcgcgcaa agagctgcaa cgtggccgcg
    12421 agctcctcct cgaaaagatc cgcgagatga agccacgcgt ggtcgcgctc gtcggcatca
    12481 cgctctatcc actcgtgctc ggcctcaagg gcaagctcgc ccaccccggc ccaggcgcaa
    12541 agcccgaaac gctcgaaggc gcgcgcgtgt tcgtggtgcc aaacccgagc ggcctcaacg
    12601 cgtcattccc gacgttcgcc gcaaagctgc cgtggttcga agcgctgggc gactacgccg
    12661 gaatgtccgg ccagccatcg ccgcgatcga agtagtcaac gccggcgcgg atcgcgaagc
    12721 ccttgtcggc agggccatgt ccgacgtgga cgctcctcgc aagatcgaca tcgtgcgcac
    12781 gcgcaaacgc taacgcgagg ccgggcatcg gcttgcggca ccagcagacc ggcggtccag
    12841 cgggatgcac gcaccgca
//
LOCUS       DATLBZ010000185         5422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_922801, whole genome shotgun sequence.
ACCESSION   DATLBZ010000185 DATLBZ010000000
VERSION     DATLBZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922801"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1194)
                     /locus_tag="VMZ53_09690"
     CDS             complement(19..1194)
                     /locus_tag="VMZ53_09690"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HUS28773.1"
                     /translation="MVTAERTREFRARCLRCRKPSSMCYCALLPRLETKTRVVILQHR
                     RERDMPIGTARMATLCLPQAELHVGVDWNNSEPLAEALSDPARPPVLLYPGPDARDIL
                     REPPPGPVTLVVVDGTWSQARTVVRDNPLLNALPRYAFAAPEPSRYRIRREPRAEYCS
                     TIEALMHVLGVLEGDPTKFRALLVPFEAMIDAQLACEASSPAQRCRYASTRPKRPNLP
                     ETIASRWDDLLCVVGEANAWPYQAGAPTRPSELVHWLAYRPSDGSLFDAISAPRGPLS
                     PSTTFHSELSEAQIAAGMPADTLIDQFAAFVRPNALVCVWGNYAPSLLRDRADVLPAE
                     RLDLRQVAGRYTQGKVGGLDKYAGDDVTPLAAGRGGRRLAMLAKLLRTWRDALEAAR"
     gene            complement(1222..2256)
                     /locus_tag="VMZ53_09695"
     CDS             complement(1222..2256)
                     /locus_tag="VMZ53_09695"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUS28774.1"
                     /translation="MRTRFLLLALTACGRLGFDATPTPMPGTDGDSDGPSSDTSSPMS
                     AKSCESLPATCGPTGNASCCESLTVPGGTFFRSYDSVGYNTMTYPATVSPFRLDKYEV
                     TVGRFRAFVDAGFGTQANPPTTVAFTTANHAVWDPMWTAKLVASSAALKTAINCFGTP
                     TWTASPGANENRPMNCITWYEAQAFCIWDGGYLPTEAEWNFAAAGGDEQRAYPWSNPP
                     TSLMIDTGYAAYSPDGGTTCPVDGNTACDVNDLLPVGSKPAGDARWGHSDMGGNVWEW
                     TMDWYVTPYATTTCNDCVTLTNATGYQVIRGGCWNYSPAGERVGARAYDPDGPPDHDA
                     TAGVRCARPI"
     gene            2319..2537
                     /locus_tag="VMZ53_09700"
     CDS             2319..2537
                     /locus_tag="VMZ53_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28775.1"
                     /translation="MPPIEEHVSRIVERLRKDHPDPAQREQLYEEDGRLDAYVATLMQ
                     GQDADEADVARIAKAVRAAEDGKPYHDA"
     gene            2561..3211
                     /locus_tag="VMZ53_09705"
     CDS             2561..3211
                     /locus_tag="VMZ53_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUS28776.1"
                     /translation="MRIIITGVTGMVGEGVLLECLENASVEKVLAVSRKPTNHTHAKL
                     EQLLVPDFRETTSVEAQLTGYDACFYCAGVSSVGMSEADYTTISYDTPVAFATTLARL
                     NPSMVFTYVTGMRTDSTEKGKTMWARVKGRAENALMRLPFKGVYNFRPGLMTPKPGQK
                     HLKTSYRVALVFAPVLKIFVRALTLSQVGRAMIRCVESGAPKQLLEIDDIAALADR"
     gene            complement(3208..3573)
                     /locus_tag="VMZ53_09710"
     CDS             complement(3208..3573)
                     /locus_tag="VMZ53_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28777.1"
                     /translation="MPPRTKARVTVDDVRTLAATLPRSYEVTVRGCVKFRVGQLVYVA
                     FDRDQTTMGFAFPKDLRASLVDSDPDKFHLPRASDLRFNWVCARLSALSRKEMPALVI
                     SAWRMCVPKKVYAAYAAQF"
     gene            3624..3866
                     /locus_tag="VMZ53_09715"
     CDS             3624..3866
                     /locus_tag="VMZ53_09715"
                     /inference="COORDINATES: protein motif:HMM:NF021429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HUS28778.1"
                     /translation="MSDEQPRNPLHGITLERIVTELVEFFGWPELGQQVRIRCFNENP
                     SVASSLKFLRKTPWAREKVESLYLFMLREKKRQQQS"
     gene            complement(3829..4506)
                     /locus_tag="VMZ53_09720"
     CDS             complement(3829..4506)
                     /locus_tag="VMZ53_09720"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HUS28779.1"
                     /translation="MLCASCRLHASLCICALVPRLHTRTRLVLLVTQSEARKPSNTGQ
                     LAARCLANSTVEIVRKNSGPLQAPAIHAGELPLVLYPAPHAIPLTAQAASRSVALIVP
                     DGSWHQARALQQRAALRNHTCVTLPPLGPSEYRLRSEPQAGGLATFEAIARALRILED
                     DDAIADTMLHVFRTMVERTLWFRGKLHAHEVTGGIPAAALANDPRGTATRAAALTTAA
                     VASSREA"
     gene            4542..>5422
                     /locus_tag="VMZ53_09725"
     CDS             4542..>5422
                     /locus_tag="VMZ53_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUS28780.1"
                     /translation="MSTPATRARWALELEARAAAYWTPERTAQLLDGKQPIIAPRDGA
                     VLLRALGLMREDASIPPAEVRKYFQINHMVALLGPALRALREQGRPIRLIDAGCGRSY
                     LTLLLAWCARHVWRHPLEVIGIDRDPALIEEARRRTQLAELDDVVRFAVGDVATMERP
                     QGVNGVVALHACDTATCHALALGIELEVEMMAVAPCCQAELARGWASLAERGVQGAFA
                     PVWGAPHLRRETAADITDAMRVELLRAAGYEVTAMEFVPAEHTRKNTLIRAIRRGAPS
                     EGARAAYQALRDATGGI"
BASE COUNT          895 a         1592 c         2008 g          927 t
ORIGIN      
        1 cgacgacgcc ctgaagactt aacgcgcggc ttcgagcgcg tcgcgccacg tgcgaagcaa
       61 cttcgcgagc attgcgaggc gacggccacc gcgaccagcg gcgagaggcg tgacgtcgtc
      121 gcccgcgtac ttgtcgaggc cgccgacctt gccttgtgtg tagcggccag cgacctgccg
      181 cagatcgagg cgctcggcgg gcagcacgtc ggcgcgatcg cggaggagcg acggggcgta
      241 gttaccccag acgcagacga gagcgttcgg ccgcacgaat gctgcgaatt gatcgatgag
      301 agtgtctgcg ggcatgcccg ccgcgatctg ggcctcgctg agctcgctgt gaaaggtcgt
      361 gctcggcgag agcgggccgc gcggtgctga aatcgcatcg aagaggctgc cgtcgctggg
      421 acgatatgcg agccagtgga cgagctccga ggggcgagtc ggtgcgcctg cttggtacgg
      481 ccacgcgttc gcctcgccga cgacgcagag caggtcgtcc cagcgcgatg cgatcgtttc
      541 gggaaggttc ggccgtttcg gccgcgtcga tgcgtaacga cagcgctgtg ctggggagct
      601 cgcttcgcac gcgagctggg cgtcgatcat cgcttcgaat gggacgagga gggcgcggaa
      661 ctttgtcggg tcaccttcga gcacgccgag cacgtgcatc agcgcttcga tcgtcgagca
      721 gtattcggcg cgcggctcgc gtcgaatgcg atagcgcgag ggctcgggtg ccgcgaatgc
      781 gtagcgcggt agcgcattga ggagcgggtt atcgcgcacg acggttcggg cttgcgacca
      841 cgtgccgtcg accacgacga gcgtgacggg gcctggcggt ggttcgcgca ggatgtcgcg
      901 cgcgtccggg ccgggataga ggaggacggg cggacgggcc gggtcgctga gtgcttctgc
      961 gagaggctcg ctgttgttcc agtcgacgcc gacgtggagc tcggcctgcg ggaggcagag
     1021 ggttgccatc cgagcggtgc cgatcggcat gtcgcgctcg cggcggtgct ggaggatgac
     1081 gactcgcgtc ttcgtctcga ggcgcggcag cagcgcgcag tagcacatcg acgacggctt
     1141 acgacagcgc aggcaacgcg cacgaaactc gcgcgttcgt tcggcagtta ccacgcctca
     1201 gcgtagcact aggagtgcac gttagatcgg acgcgcgcag cgcacgcctg cagtcgcatc
     1261 gtgatcgggc ggaccgtctg gatcgtaggc acgggcgccc acgcgctcgc cggcaggcga
     1321 atagttccag caaccgccgc ggatgacttg atatccggtt gcgttcgtca gcgtgacgca
     1381 gtcgttgcag gtcgtcgtgg catacggcgt cacgtaccaa tccatcgtcc actcccaaac
     1441 gttgccgccc atgtcggaat gtccccagcg cgcgtcaccc gcgggcttcg agccaacggg
     1501 caggaggtcg ttgacgtcgc acgcggtgtt gccgtcgacg ggacaggtcg tgccgccatc
     1561 gggcgagtac gcagcgtagc ccgtgtcgat catcagcgac gtcggagggt tcgaccaggg
     1621 atacgcgcgt tgctcatcac cgccagccgc cgcgaagttc cattcggctt cggtcgggag
     1681 gtagccgccg tcccagatgc agaacgcctg cgcttcgtac caggtgatgc agttcatcgg
     1741 ccgattctcg ttggcacctg gcgacgcggt ccacgtcggc gttccaaagc agttgatcgc
     1801 cgttttcagc gcggccgagc tcgcgacgag ctttgcggtc cacatcggat cccagacggc
     1861 gtggtttgcc gtggtgaaag cgacggtcgt gggcggattc gcttgggtgc cgaacccggc
     1921 gtcgacgaag gcgcggaagc gaccgaccgt cacctcgtac ttgtcgagcc ggaacgggct
     1981 caccgtcgcc gggtacgtca tcgtgttgta accgacgctg tcgtagctgc gaaagaacgt
     2041 gccaccgggg accgtcaggc tctcgcagca cgacgcgttg ccggtgggac cgcacgtcgc
     2101 cggcagggac tcgcagcttt tggcgctcat cggcgacgac gtgtcgctag aagggccgtc
     2161 gctatcgccg tcggttcccg gcatgggcgt cggcgtcgcg tcgaagccga ggcgaccgca
     2221 cgcggttagc gcaagcaaca ggaaccgcgt tcgcacgtaa ccactctatc actcgcgcga
     2281 gcacgtgctc gagatcgacg acattgctac gctggatcgt gccgccaatc gaagagcatg
     2341 tgagtcgcat cgtcgagcgc ctgcgcaaag atcacccgga tcccgcgcag cgcgagcagt
     2401 tgtatgaaga ggacggccgg ctcgatgcgt acgtcgcgac gctgatgcag gggcaagacg
     2461 ccgacgaggc ggacgtcgcg cggatcgcga aggcggtgcg cgccgctgaa gatggcaagc
     2521 cgtatcacga cgcgtagcga gggcgcggta caacccgcgg atgcgcatca tcatcaccgg
     2581 cgtgactggc atggtcggcg aaggcgttct gctcgagtgc ctcgagaatg cgtcggtcga
     2641 gaaggtgctg gcggtgtctc ggaagccgac gaatcacacg cacgcaaagc tcgagcagtt
     2701 gctggttccc gattttcgcg agacgaccag tgtcgaggcg cagctgaccg ggtatgacgc
     2761 gtgcttttac tgcgcgggcg tcagctcggt cgggatgagc gaggccgact acacgacgat
     2821 cagctacgac acgccggtgg cgtttgcgac gacgttggcg cggctcaatc cgagcatggt
     2881 cttcacgtac gtgacgggca tgcggacgga cagtacagag aagggcaaga cgatgtgggc
     2941 gcgcgtgaag ggccgtgcgg agaacgcgct catgcgcttg ccgttcaagg gcgtctacaa
     3001 ctttcggccg ggcctcatga cgccgaagcc cgggcagaag catctgaaga cgtcgtatcg
     3061 cgtcgcgctc gtgttcgcgc cggtgttgaa gatcttcgtt cgtgcgctca cgttgtcgca
     3121 ggttggtcgc gcgatgattc ggtgtgtcga gagtggcgcg ccgaagcagt tgctcgagat
     3181 cgacgacatc gccgcgcttg ccgaccgcta gaattgcgcg gcgtaggccg cgtagacctt
     3241 cttggggacg cacatgcgcc atgccgagat gacgagcgcc ggcatctctt tgcgagagag
     3301 cgccgagagg cgcgcgcaga cccagttgaa gcgcaggtcg gacgcgcgcg gcaggtggaa
     3361 cttgtctgga tcggagtcga cgagcgacgc gcggaggtcc ttggggaacg cgaagcccat
     3421 cgtggtttgg tcgcgatcga acgcgacgta gacgagctgg ccgacgcgga acttcacgca
     3481 gccgcggact gtgacctcgt acgaacgcgg cagcgtggcg gcgagcgtgc gcacgtcgtc
     3541 gacggttacg cgagccttcg tgcgaggagg cacgtcgaga cgatggcaca cggcgagcgg
     3601 tcgcggttca atcggggctg cacatgtccg acgaacagcc ccgcaatcct ttgcatggca
     3661 tcacgctcga gcggattgtt acggagctcg tcgagttctt cgggtggccc gagctggggc
     3721 agcaggtgcg gatccgttgc ttcaacgaga atccgagcgt ggcgtcgagc ttgaagttct
     3781 tgcgcaagac accgtgggcg cgcgagaagg tcgagagcct ctacttgttc atgcttcgcg
     3841 agaagaagcg acagcagcag tcgtgagggc ggcggcgcgc gttgcggtgc cgcgtggatc
     3901 gttggcgagc gcggcggctg ggatgccacc ggtgacttcg tgcgcgtgca gcttgccgcg
     3961 aaaccacagc gtgcgttcga ccatcgtgcg gaagacgtgg agcatcgtgt cggcgattgc
     4021 atcgtcgtct tcgagaatgc gcagagcgcg ggcgattgcc tcgaacgttg cgaggccgcc
     4081 ggcttgtggc tcggagcgga ggcgatattc gctcgggccg aggggcggca gcgtcacgca
     4141 cgtgtgattg cggagcgcgg cgcgttgttg cagggcgcgg gcttggtgcc agctaccgtc
     4201 gggaacgatc agcgcgactg atcgcgaggc ggcttgcgcg gtgagcggga tggcgtgtgg
     4261 cgcgggatag aggacgagtg ggagctcgcc ggcgtggatc gcgggggctt gcagcgggcc
     4321 actgttcttg cgcacgatct cgaccgtgct gttggcgaga cagcgcgccg cgagttggcc
     4381 ggtgttgctc ggcttgcgcg cttcgctctg ggtgacgagg aggacgaggc gcgtacgcgt
     4441 gtgcaaccgc ggaacgagcg cgcagatgca caggctcgcg tggaggcggc agctcgcgca
     4501 caacatggtg cgaactgtag cgcgagagct atgctgcagc gttgtcgacg ccagccacac
     4561 gtgcgcgatg ggcactcgag ctcgaggcgc gggctgctgc gtattggacg ccggaacgca
     4621 cggcgcagct cctcgacggc aagcagccga tcattgcgcc tcgcgatggc gcggtgctgc
     4681 tgcgtgcgct cggcttgatg cgtgaggacg catcgattcc gccggctgaa gttcgcaaat
     4741 attttcagat caatcacatg gtcgcgctgc tcgggccggc actgcgggcg ttgcgcgagc
     4801 aaggacggcc gatccggctg atcgatgctg gatgcgggcg gtcgtatttg acgctgttgc
     4861 tggcgtggtg cgcgcggcat gtgtggcgac atccgttgga ggtgatcgga atcgatcgcg
     4921 atccggcgct gatcgaggaa gcgcgacggc gtacgcagct tgcggagctg gacgatgtcg
     4981 ttcggtttgc cgtgggtgat gtggcgacga tggagcggcc gcaaggcgtg aatggcgtcg
     5041 tcgcgctgca tgcgtgcgat acggcgacgt gtcacgcact cgcacttggc atcgagctcg
     5101 aggtcgagat gatggcggta gcgccatgtt gtcaggcgga gcttgcccgc ggatgggcgt
     5161 cactcgcgga gcgtggcgta cagggtgcgt ttgcgccggt ctggggagcg ccgcacttgc
     5221 gtcgcgagac cgcggcggac atcaccgacg cgatgcgcgt cgagcttctg cgcgccgctg
     5281 gctacgaggt gacggcgatg gagttcgtgc ccgccgagca cacgcggaag aacacgctga
     5341 tccgggcgat tcgccggggt gctccgagcg agggcgcgcg agcggcgtat caggcgcttc
     5401 gcgatgcgac cggcgggatt ga
//
LOCUS       DATLBZ010000186         3661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5394555, whole genome shotgun sequence.
ACCESSION   DATLBZ010000186 DATLBZ010000000
VERSION     DATLBZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5394555"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VMZ53_09730"
     CDS             complement(<1..834)
                     /locus_tag="VMZ53_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28781.1"
                     /translation="MRIIFVLSFVLAATPAHAEDRAAAERFFRAGSKAYAAQSFQAAA
                     SNFDEAFKALQMPEIAFSAAQAFRKLYQVEPKPEHVKRAIELYEFYLAKVKTGGRVSD
                     AADNLAEMKRERDKLEAAGIHASSVVEKPQTRLVINVGVADQASSADSGTLREIGDVG
                     ADSTIKGLVTTIDGQKVEPFTPAPVDAKEHVIAVAADGYFSLEKKTVAVDAQTTLVDI
                     ELQPKPAKLTVKTDDDAKILVDGRALATAPAAALEVPHGKHLLSVLRRGREPFAQEVT
                     VG"
     gene            complement(834..3506)
                     /locus_tag="VMZ53_09735"
     CDS             complement(834..3506)
                     /locus_tag="VMZ53_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HUS28782.1"
                     /translation="MKTASLWIALGVAFTAAACTDFSTPDRGVCGNGLLEPGEDCDSS
                     DATCVKCAVACSTSAQCPSSAYACGVDGFCHAPGGELDDLHGAGVFQAEQLRVTDVDH
                     DGIGDALGVSSTSLLVRRGDAAGQLAMLETVLTPSQTGPATFGDLDGDGSLDVSLATP
                     DGLVSYASTFGALAPIPSSEGIFDEDDGGALQLHKILYLGPNVLAALAVDGQGKLGVF
                     SIDGTAPQAARNLGRDQQFAYPCQTRVGLISASEIESIDVYNVSTKTDARMDTIVALR
                     SGSGANRRVCIVSVHRDAPNIFALPQNYPIITFNDITPAALGTPTQRPVLADLDTDNG
                     DACPGLVTWDGGLNGMKYWEASTPTSGAECAFAAAATNLPALPSSPTAKLVGAAPVSP
                     TVPLVAADGLASSEALFVYVPGGVPFSFPTQYWAVVYYTTRTIGRVAHGDVNGDGQVD
                     VVLTAQGEANLDVLFRQQGEAGFNLVRMETLAAVTNLVIGDYDANQVADIAYTELADD
                     HERMMVAFGTADRPLEPMLMGVFSAISNVERLGIPDSVDYLGLADDLVVTMPPSQGQT
                     ASRLTILHGSAQRTLISYFDPRSDAYKGQGQVFRGTVIGDFVATGQAPGLADLVAIGA
                     DASSTASGGVRAWKVLGTADGLDATKNDGFALQGIPSCTAAPGSAKCETHYLAVPTAP
                     DHDVVIGVDSARHALFVDPWSTSNTAVALDELVAAIPAGSIVRSLHAADLDGDGSVEV
                     VAAFAPERGNVNAASAILVCSTSAGMPHDCKNLVPEIQAAATSAELSIAQCYDATIAR
                     ITARDPAGAPAAGTNIVVACRGDGTSLFRVRRVDGEDTVDHLATFAGDVSALAAGDVT
                     GDGVDDLVMLQGDAVRSLLVYRQCTSRDCAGGSQ"
BASE COUNT          599 a         1244 c         1197 g          621 t
ORIGIN      
        1 gcccacggtg acttcctgcg cgaatggctc gcggccgcgg cggagcaccg agagcagatg
       61 cttgccgtgc ggaacctcga gtgcggcggc cggcgcggta gcgagcgcgc gtccgtcgac
      121 gaggatcttc gcgtcgtcgt cggtcttcac cgtgagcttc gcgggcttgg gctggagctc
      181 gatgtcgacg agcgtcgtct gcgcatcgac ggcgacggtc ttcttctcga gcgagaagta
      241 gccatcggcc gcgacggcga tgacgtgctc cttcgcatcg accggtgcgg gcgtgaacgg
      301 ctcgaccttc tggccgtcga tcgtcgtgac gaggcccttg atcgtcgagt cggcgcccac
      361 atcgccgatc tcgcgcagcg tgccgctatc ggcgctactc gcctgatcgg caacgccgac
      421 gttgatcacg agccgcgtct gcggcttctc gaccacggac gacgcatgaa tgcccgcggc
      481 ttcgagcttg tcgcgctcgc gcttcatctc ggcgaggttg tcggctgcat cactgacgcg
      541 gccgccggtc ttcaccttcg cgagataaaa ttcgtacagc tcgatcgcgc gcttgacgtg
      601 ctcgggcttg ggctcgactt gatagagctt gcgaaacgcc tgcgcggcgg agaacgcgat
      661 ctccggcatc tgcagcgcct tgaacgcttc gtcgaagttc gacgcagcgg cctggaagct
      721 ctgcgccgcg tacgccttgg agcctgcgcg aaagaaccgc tcggcggcag cgcgatcttc
      781 ggcgtgcgcg ggcgttgctg cgagcacgaa cgagagaacg aaaatgatgc gcatcattgc
      841 gagcctcctg cgcagtcacg actagtgcat tggcgataga cgagaagcga tcgcacggcg
      901 tcgccttgca gcatcacgag gtcgtcgacg ccatcgccgg tgacatcgcc tgctgcgagc
      961 gcgctgacat cacctgcgaa tgtcgcgagg tggtcgacgg tatcctcgcc atcgacgcgg
     1021 cgaacgcgga acaggctcgt gccgtcgccc cggcatgcga cgacgatgtt cgtacccgcc
     1081 gccggcgcac ccgccggatc tcgagcggtg atgcgcgcga tcgtcgcgtc gtagcactgc
     1141 gcgatcgaga gctctgcgct cgtggctgcg gcctggattt ccgggacgag gttcttgcag
     1201 tcatgcggca tgcctgccga tgtcgagcac acgaggatcg cgctcgctgc gttgacgttg
     1261 ccgcgctcgg gggcgaacgc cgcgacgacc tcgacgctgc catcaccgtc gagatctgcg
     1321 gcatgcagcg agcgcacgat cgagcccgct gggatcgcag ccacgagctc gtcgagggcg
     1381 accgccgtgt tgctcgtgct ccatggatcg acgaacagtg cgtggcgtgc gctgtcgacg
     1441 ccgatgacaa catcgtggtc gggcgccgtc ggcactgcga ggtagtgcgt ctcgcacttc
     1501 gcagatccgg gcgccgccgt gcacgacggg attccctgga gcgcgaagcc atcgttcttc
     1561 gtggcgtcga ggccatcggc agtgccgaga accttccacg cgcgcacacc gccgctcgcg
     1621 gtgctcgatg cgtccgctcc gatcgcgacg aggtcggcga gccccggtgc ttggcccgtc
     1681 gcgacgaagt caccgatcac ggtgccgcgg aacacctggc cctgaccttt gtacgcgtcg
     1741 ctacgcgggt cgaagtacga aatcagcgtg cgctgtgcgc tgccatgcag gatcgtcagc
     1801 cggctcgcgg tctggccttg cgacggcggc atcgtgacga cgaggtcgtc ggcgagcccg
     1861 agataatcga cgctgtcggg aatgccgagc cgctcgacgt tcgagatggc ggagaacacg
     1921 cccatgagca tcggctcgag cgggcgatcg gccgttccga acgcgaccat catgcgctcg
     1981 tgatcatccg cgagctcggt gtacgcgata tcagcgacct gattcgcatc gtagtcgccg
     2041 atgaccaggt tcgtgaccgc agccaacgtc tccatccgca cgaggttgaa gcccgcttct
     2101 ccctgctgcc gaaacaacac gtcaaggttc gcttcgccct gcgcggtgag cacgacatcg
     2161 acttgcccat cgccgttgac gtcgccgtgc gcgacgcggc cgatcgttcg cgtcgtgtag
     2221 tagacgacgg cccaatactg cgtcggaaac gagaacggca cgccgccggg cacgtacacg
     2281 aacaacgcct ccgacgacgc gaggccatcc gccgcgacga gcggcacggt tggcgagacg
     2341 ggcgcggcac cgacgagctt tgcggtcggt gagctcggca acgcgggcag gttcgtcgct
     2401 gctgccgcaa acgcgcattc cgcacccgac gtcggcgtcg acgcctccca gtacttcatg
     2461 ccgttgagcc cgccgtccca cgtgacgagg ccaggacacg cgtcgccgtt gtccgtgtcg
     2521 aggtcggcga gcaccgggcg ctgcgtcgga gtgccgagcg ccgcgggcgt gatgtcgtta
     2581 aacgtgatga tcggatagtt ctgcggcagc gcgaagatgt tcggcgcatc gcgatgaacg
     2641 gacacgatgc acacccgtcg gttcgcgcca ctgcccgagc gcagcgcgac gatcgtatcc
     2701 atccgcgcgt cggtcttcgt cgatacgttg tacacgtcga tcgactcgat ctcgctcgca
     2761 ctgatcaggc ccacgcgcgt ctgacacgga tacgcgaact gctgatcgcg tccgaggttc
     2821 ctcgccgcct gcggcgcggt gccgtcgatc gagaacacgc cgagcttgcc ttgcccgtca
     2881 acggctaacg ccgcgagcac gttcggtccc aaatagagaa tcttgtgcag ctggagcgcg
     2941 ccgccgtcgt cctcgtcgaa gatgccttcg ctcgatggga tcggcgcgag tgcgccaaac
     3001 gtcgacgcat acgagacgag cccgtcgggc gtcgcgagcg agacgtcgag tgacccatcg
     3061 ccatcgagat cgccaaacgt cgccggaccg gtctgcgacg gcgtcagcac cgtctccagc
     3121 atggcaagct ggcccgctgc atcgccgcgg cgcacgagca gcgacgtgct cgacacgccg
     3181 agtgcatccc cgatcccatc gtgatcgacg tcggtcacgc gcagctgctc ggcctgaaag
     3241 acgccagcgc catgcaggtc gtccagctct ccgcccggcg cgtgacaaaa cccgtcgacg
     3301 ccgcacgcgt acgcactcga cggacattgc gcgctcgtcg agcacgcaac ggcgcacttc
     3361 acgcacgtcg cgtcgctcga gtcgcaatcc tcgccaggct cgagcagccc atttccgcag
     3421 acgccgcggt ccggcgtcga gaagtcggtg cacgccgccg cggtaaacgc gacgccgagc
     3481 gcaatccata agctagcggt cttcatttct tccctccgcc aaagacgtcg atgatgtcgt
     3541 cgcgaccagg ccggtgcccg ccgccgctcg ccttcggctt cttcttgagc gccgcgtcga
     3601 gcagcttgtc gccatcgggc acgacgctct tcgacgtcgg caaatacccg tcggcgcgaa
     3661 a
//
LOCUS       DATLBZ010000187         6113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4644764, whole genome shotgun sequence.
ACCESSION   DATLBZ010000187 DATLBZ010000000
VERSION     DATLBZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4644764"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2319)
                     /locus_tag="VMZ53_09740"
     CDS             complement(<1..2319)
                     /locus_tag="VMZ53_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS28783.1"
                     /translation="MGSLLLDLRYSLRMMAKTPALTAILAITLALGIGASTTIFSVVN
                     SVVLKDLPYDEPDHLVRVYTGFKGAGQNTPRFPFSVPEIGELQQACRSCSLVAGWLPG
                     TAPLSGGDRPVRITVGYATHQLLPLLGVPPLMGRWFDASEDSPQKWVPGAQIVGDPSV
                     VVINYEIWKSVFGGDPNIIGRKVELDAIPVTIVGVMPEGFKFDDMEAWVPARLDFTSL
                     RRNSHSGNVLARLAPGASLESLNQELAALTKEWGKRDTKDFHSIKEDHPLEAVPFKQD
                     MVGSISSVLWMLQGAVLFVLLISIVNIANLLLARAETRTREIAVRHALGASRRRLLRQ
                     FVTESLVLGILGAGLGILVSVWALDGVTALIPKSAPRAAEIKLDGTAVVFAVCCSLVA
                     ALLFGLAPILHAKKTDLHGALKDGSNRMTGSRARLRVRRALVIVEIALAMLLVIGCTV
                     MVRTFVKLQRVELGFKPNNVLTFGINLPLRTYEQPTVALQFWDRLEARLNSMPGVDGA
                     ALVESFGTDRGMNMEGIRIAGRPESTDSAPADVVEYVQMVSPKALTVMGARFTRGRDL
                     SDSDTATAALVMVVNESFVKKFFPNEDPIGKEVVPFPQQDPSKDKRVRIVGVFADMKN
                     RGLEKPSGTEIVMVRAQVPTLFDPPMSPLVMYGVVRSSDSPAELAPAVARVVAEIDPA
                     IPVYQMRTMNDVLWEAVSRPRFLTLILTAFAFVALVLAAVGIYGVMAHTVAQRTHEIG
                     LRVALGAQPAQVRRLVLRQAASLVATGVSIGVA"
     gene            complement(2319..4820)
                     /locus_tag="VMZ53_09745"
     CDS             complement(2319..4820)
                     /locus_tag="VMZ53_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS28784.1"
                     /translation="MGSLWLDIRYALRMMVKTPGLTAVLAITLALGIGASTTIFSVVN
                     SVVLRPLPYERPDQMVRVYTEFKGQLGLHNFWVSGPEYDDLRKACRTCKSVAAWSRGT
                     ASLAGGDRPVRVEAAYGTANLLPLIGVKPMLGRWFDESEDGPGGDPSVVVLGYDVWKR
                     AFGGDKRVVGRSIHLDAMPVTVIGVMPPGFDFLDREEAWVPLHLDVSKTNRGGHFLNV
                     VVRLNDDASIKAFNDELAALRIEWAKLRAPNVHAITDEGHPMVARPFQADLVGNLATT
                     LWLLQGAVLLVLLISIVNVANLLLARSETRTREVAVRHALGASRTRLVRQFLTESMLL
                     AIVGGGLGVLVAVWAVDGVTALIPKSAPRASEITLDAKAVVFAVACAVAAALLFGMAP
                     ILHARRTNLYSALKDGSNRMTASRARLRVRRGLVIGEIALAVLLVIGCTVMVRSFLKL
                     QEVKLGMKPDHLLTFEIELPTKKYPGTTGSQFWERLQDRLRALPGVTNATLLDGLPPS
                     RPINANDIDFPDKTQPVGPPGFGLVWNVDYWQVVGDDMEGALGARIVKGRPIQASDTA
                     TAPSVVLVNEAFANRFFPGEDPIGQRVVIWGNRHGDPKALKQTIVGVVGNYKQAGIEK
                     PAGTEVFVPWRQYQQLCDKPEECSAPTSLFAVVRTTSNPTTLTASIHHVVADLDPSLP
                     VSQLRSMDDVMWEAVARPRFLTFLLIAFAAIALLLAAVGIYGVMAHTVAQRTHEIGLR
                     VALGAQPSQVRAMVLRQAATLVAAGIAIGLGVAIGLQEILDASLQNLFYGERMSQPIL
                     LIGVALAVTLTAMLATWIPARRATKVEPTVALRSE"
     gene            5055..>6113
                     /locus_tag="VMZ53_09750"
     CDS             5055..>6113
                     /locus_tag="VMZ53_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28785.1"
                     /translation="MLRIKLRYEDLDAMVQRFAPNVGKSGLFLPTKSIQPIGTEVKFE
                     LRLANDTPALIGIGKVKHVKRPDPANPKAAFGMAIELMRVSREGREVIIRMIERRRAM
                     GLADVAIPMPEDTETARRSEVETQPRADTSGIVKDAMQQFASAPVSEVVLATPPQSGP
                     IAVPKEPSRPNTAAPLLTSARDSGPISREVSKSVPVLAPEAPRAKRPRVSDLIAKATE
                     LSGPLASMPVPGLDEHVDVEAALVRARALAAGTDLDAELAGLVDSAATPVEISIDAAS
                     AELARQLGGVAIAKRDRSARWAPPPAVETKPPPPVEEPAVADEFAHKTRAETEASAAS
                     VIAAVIDKAVEAAPPPDPA"
BASE COUNT         1090 a         1967 c         2106 g          950 t
ORIGIN      
        1 cgcgacaccg atgctcacgc cggttgcgac gagtgacgcc gcttggcgaa gcacgagacg
       61 ccgcacttgg gccggttgtg caccgagcgc gacgcgcagg ccgatctcgt gcgtgcgctg
      121 cgcgacggta tgcgccatga ctccgtagat gccgacagcg gcgagcacga gcgcgacgaa
      181 tgcgaatgcg gtgaggatca gcgtcaggaa gcgcggccgc gagaccgctt cccagagcac
      241 atcgttcatc gtgcgcatct gatagacggg aatcgccgga tcgatctcgg cgacaacgcg
      301 tgcgacggcg ggtgcgagct cggctggcga gtcgctcgag cggacaacgc cgtacatcac
      361 gagtggcgac atcggcggat cgaacagcgt cggcacctgc gcgcgcacca tcacgatctc
      421 cgtgcccgac ggcttttcga ggccgcggtt cttcatgtct gcgaagaccc cgacgatgcg
      481 aacgcgcttg tccttgctcg gatcctgctg cgggaacggc acgacttctt tgccgatcgg
      541 atcttcattc gggaagaatt tcttcacgaa cgattcgttg acgaccatca cgagcgccgc
      601 ggtcgcggta tcgctgtcgc tgaggtcgcg accacgcgtg aagcgcgcgc ccatcacggt
      661 cagtgccttg gggctgacca tctgcacgta ctcgacgaca tctgcgggcg cgctgtccgt
      721 cgactcgggg cggcctgcga tgcggatgcc ttccatgttc atgccgcgat cggtgccgaa
      781 gctctcgacg agcgccgcac cgtcgacacc gggcatcgaa ttcagccgcg cttcgagccg
      841 atcccagaac tgcagtgcga cggtcggctg ctcgtacgtg cgcagcggca agttgatgcc
      901 gaacgtcagc acgttgttgg gcttgaagcc gagctcgacg cgctggagct tcacgaacgt
      961 gcggaccatg acggtgcagc cgatcacgag cagcatcgcg agtgcgatct cgacgatgac
     1021 gagcgcgcga cggacgcgca ggcgagcgcg cgagccggtc atacgattgc tgccgtcttt
     1081 gagcgcaccg tgcaggtccg tcttcttggc atggagaatc ggcgcgagac cgaacagcag
     1141 tgcggcgacg agcgagcagc acacggcgaa gaccactgcg gtgccgtcga gcttgatctc
     1201 ggccgcgcgc ggcgctgact tgggaattag cgcggtgacg ccgtcgagcg cccacacaga
     1261 cacgaggatg ccgaggcccg cgccgaggat accgagcacg aggctctcgg tgacgaactg
     1321 gcgaagcagg cggcgacgcg atgcaccgag cgcgtgacgc acggcgatct ctcgcgtccg
     1381 cgtctcggcg cgagcgagca ggaggttcgc gatgttgacg atcgagatca gcaagacgaa
     1441 caggaccgcg ccctgcagca tccacagcac cgacgagatc gagccgacca tgtcttgttt
     1501 gaacggcacg gcctcgagtg ggtggtcttc cttgatcgag tggaagtctt tcgtgtcgcg
     1561 cttgccccat tccttcgtga gcgcggcgag ctcctggttg agcgactcga gcgaagcacc
     1621 gggcgcgaga cgcgcgagca cgttgccgga atggctgttg cgacgcagcg acgtgaagtc
     1681 gaggcgcgcg gggacccagg cttccatgtc gtcgaacttg aagccctcgg gcatcacgcc
     1741 gacgatcgtc acgggaatcg cgtcgagctc taccttgcgg ccgatgatgt tcggatcgcc
     1801 gccgaagacc gacttccaga tctcgtagtt gatgacgacg acgctcgggt caccgacgat
     1861 ctgcgcgccg ggtacccact tctgtggcga gtcctcgctc gcgtcgaacc agcggcccat
     1921 gagcggcggc acgccgagga gcggaagcag ctgatgcgtc gcatacccga cggtgatgcg
     1981 aacgggccgg tcgccaccag acagcggggc cgtgccgggc agccagccgg cgacgagcga
     2041 gcacgaccgg cacgcctgct gcagctcgcc gatttccggg acggagaacg ggaagcgagg
     2101 cgtgttctgg ccggcgcctt tgaagccggt gtagacgcgg acaagatggt cgggctcgtc
     2161 gtacggcaga tccttcagga cgaccgagtt gacgacggag aagatcgtgg tgctggcacc
     2221 gatgccgagc gcgagcgtga tcgcgaggat ggcggtgagc gctggcgtct tcgccatcat
     2281 gcgaagcgag tagcgaagat cgaggagcag cgagcccatc actcgctcct gagcgccacg
     2341 gtgggctcga ccttcgtcgc gcggcgcgcg gggatccacg tcgcgagcat cgctgtgagc
     2401 gtgacggcga gcgcgacgcc gatgagcaag atgggctggc tcatgcgctc gccgtagaac
     2461 agattctgga gcgacgcgtc gaggatctct tgcagaccga tcgcaacgcc gaggccgatc
     2521 gcgatgccgg ccgcgacaag ggtcgctgct tggcggagca ccatcgcgcg gacttgcgac
     2581 ggctgtgcac cgagcgcgac gcgcaggcca atctcgtgcg tacgctgcgc gacggtgtgc
     2641 gccatcacgc cgtagatacc gaccgcggcg agcagcagcg cgatcgcggc gaacgcgatc
     2701 agcaggaacg tgaggaagcg aggacgcgcg acggcttccc acatgacgtc gtccatcgag
     2761 cgcagctgcg agacgggcag cgacggatcg agatccgcga cgacgtggtg gatcgacgcg
     2821 gtgagcgtcg tcggattgct agtcgtgcga acgacggcga acagcgacgt cggagcgctg
     2881 cactcctcgg gtttgtcgca gagctgttgg tactggcgcc acggcacgaa cacttcggtg
     2941 ccggcgggct tctcgatgcc agcttgtttg tagttgccga cgacgccgac gatcgtttgc
     3001 ttgagcgcct tcggatcgcc gtgcctgttg ccccagatga cgacccgctg gccgatcgga
     3061 tcctcgccgg ggaagaagcg attcgcgaac gcttcgttga cgagcaccac cgagggcgct
     3121 gtcgcggtgt cgctcgcttg gatcggacgg cccttcacga tgcgcgcgcc gagtgcgccc
     3181 tccatgtcgt cgccgacgac ctgccagtag tcgacgttcc agacgaggcc aaagccaggc
     3241 ggtcccaccg gctgcgtctt gtcggggaag tcgatgtcgt tggcgttgat ggggcgagat
     3301 ggaggcaggc catcgaggag cgtcgcgttc gtgacgccag gcagcgcgcg caggcgatcc
     3361 tgcaagcgtt cccagaactg cgagcccgtc gtgccgggat acttcttcgt cggcagctcg
     3421 atctcgaacg tcagcaagtg gtccggcttc atgccgagct tcacttcctg cagcttgagg
     3481 aagctgcgca ccatcacggt gcaaccgatg acgagcaaca ccgcgagcgc gatctcgccg
     3541 ataacgaggc cgcggcgaac gcgaaggcgc gcacgcgatg cggtcatgcg attgctgccg
     3601 tccttgagtg cggaatacaa atttgtgcgg cgcgcgtgga ggatcggcgc catgccgaac
     3661 aacagcgctg cagcgaccgc gcacgcaacg gcgaacacga ctgccttcgc atcgagcgtg
     3721 atctcgctcg cgcgtggtgc ggacttcggg atgagcgcgg tcacgccgtc gacggcccac
     3781 accgcgacga ggacgccgag accgccgccg acgatcgcga gcagcatgct ctcggtgagg
     3841 aactggcgaa cgaggcgcgt gcggctcgcg ccgagtgcgt ggcggacggc gacctcgcgc
     3901 gttcgagtct cgctgcgcgc caacaataga tttgccacgt tgacgatcga gatgaggagc
     3961 acgagcagca cggcgccttg caggagccac agtgtcgtcg cgaggttgcc gacgaggtcg
     4021 gcctggaacg gccgcgccac catcggatgc ccttcgtcgg tgatcgcgtg aacgtttggc
     4081 gcgcgcagct ttgcccattc gatgcggagt gcagcgagct cgtcgttgaa tgctttgatc
     4141 gacgcgtcgt cgttcagccg cacgaccacg ttcaagaagt ggccaccacg attcgtcttc
     4201 gagacgtcga gatgcagcgg cacccacgcc tcttcgcgat ccagaaagtc gaagccgggc
     4261 ggcatgacgc cgatgacggt caccggcatc gcgtcgaggt gaatcgatcg gccgacgacg
     4321 cgcttgtcgc cgccgaacgc gcgcttccac acgtcgtagc cgagcacgac gacgctcgga
     4381 tcgccgcccg gtccatcttc cgattcgtcg aaccagcgac cgagcatcgg cttgacgccg
     4441 atcagcggca gcaaattcgc ggtgccgtag gcggcctcca cgcgaaccgg tcggtcgcca
     4501 ccggcgagtg atgcagtgcc gcgcgaccag gccgcgacgc tcttgcacgt acggcacgcc
     4561 ttgcgcaggt cgtcgtactc cggccccgag acccagaagt tgtgcagacc gagctggccc
     4621 ttgaactcgg tgtacacgcg caccatctga tccggccgct cgtacggcag tggacgcagg
     4681 acgaccgaat tgacgacgga aaagatggtc gtgctggcac cgatgccgag cgccaaggtg
     4741 atcgcgagaa ccgcggtcag gcctggcgtc ttgaccatca tccgcagcgc gtaccgaatg
     4801 tccaaccaga gcgatcccat cgaccgccgc tagagcagcg tacgtgccag ctcaacctcg
     4861 cgagatcatg tggtgactcg cccgcccgcc gttcgcttca gggattggcg atcccacaac
     4921 cggacacccc agaaagcttc ggatctccac gtcgtcggcg gtttgacggc ggcgtgcgcc
     4981 gggcgagata atcagcggcg ccccaacaga tggcccccga tgacaatagc ggggcgcggc
     5041 cggcgggccc ggtcgtgctc cggatcaagc tgcgctacga ggacctcgac gcgatggtcc
     5101 agcgcttcgc gccgaatgtc ggcaagtcgg ggttgttcct gccgaccaag tcgatccagc
     5161 cgatcggcac cgaggtgaag ttcgagctgc ggctcgcgaa cgacacgccc gcgttgatcg
     5221 gcatcggcaa ggtgaagcac gtcaagcggc ccgatccggc gaatccgaag gcggcgttcg
     5281 gcatggcgat cgagctcatg cgtgtctcgc gcgagggccg cgaagtgatc atccgaatga
     5341 tcgagcggcg tcgcgcgatg ggcctggccg atgtcgcgat cccgatgccc gaagataccg
     5401 agacggcgcg gcgcagtgag gtcgagacgc agccgcgcgc agacacgagt ggcatcgtga
     5461 aggatgcgat gcagcagttc gcgtcggcgc cagtgagcga agtagtgctc gcgacgccgc
     5521 cgcagtccgg tccgatcgcg gtcccgaagg agccgagccg tccgaacacc gcagcgccgc
     5581 tgctcacgtc cgcgcgtgac tcggggccga tctcgcgcga ggtcagcaag tctgtgcccg
     5641 tcctcgcgcc ggaagcgccg cgcgcgaagc ggcctcgcgt gtccgacttg atcgcaaagg
     5701 cgacggagct ctcgggccca ctcgcgtcca tgccggtgcc ggggctcgac gagcacgtcg
     5761 acgtcgaagc ggcattggtg cgcgcacgcg cgctcgcggc tggaaccgat ctcgatgccg
     5821 agcttgcagg cctcgtcgac tctgcggcaa cgcccgtcga gatcagcatc gatgcggcga
     5881 gtgcggagct cgcgcgccag ctcggcggtg tcgcgattgc gaagcgcgat cgcagcgcac
     5941 gctgggcccc gccgccggcg gtcgagacga agccgccgcc tcccgtcgag gaaccagccg
     6001 tcgccgatga attcgcgcac aagacacgtg cggagaccga agcgagtgca gcctcggtga
     6061 tcgcggcggt gatcgataaa gccgtcgagg cggcgccgcc gccggatccc gcg
//
LOCUS       DATLBZ010000188        34354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2415103, whole genome shotgun sequence.
ACCESSION   DATLBZ010000188 DATLBZ010000000
VERSION     DATLBZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 34354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34354
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2415103"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VMZ53_09755"
     CDS             <1..522
                     /locus_tag="VMZ53_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28786.1"
                     /translation="TLLGGGDAFGDDDSIFSGFALSHDGATAFIGDSNVVGTNRVGIV
                     SVSATDIAPLAVITTNITDPSGIAASPFGDVAIVTSSQPPGEGIYVLDKGGTNGAWRN
                     RGELTYMGAASQLPGDVVTIDRGMLEGSVIVSELSKVRRLQFHADGSVTDTGSLAFGS
                     GLEEIAGAIGVTP"
     gene            671..2077
                     /locus_tag="VMZ53_09760"
     CDS             671..2077
                     /locus_tag="VMZ53_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HUS28787.1"
                     /translation="MRNAAIASLLAVVASCSAQDDEYVPTGAPDETDSTGEGGKEDSP
                     APGPEVPVAQQSADIGQFFMVDHYGVDAAGFADIRNKIKTKNLGAIILWNPTNASGET
                     LRQMIRGYSDVAKAAGRDELFYAADQEELRTQRFASANGFTSLVQGSVLGSVSSRDRL
                     CELHARITSREMASTGMNMSLGTVSDIYTSDSGTRGMFRTRAIDDQPENVASCVVAMT
                     KAYAQEGHVVYITKHFPGLGNASGNTDVDASVATRSKTKAAMERELAPYRAASASVTA
                     ANGWPFFGAMISHASYPVIDGGALPATLSSKIVNDFMRGTGNLTLGGKDSAGAATTFA
                     GMGFRGLSISDAFWTWGATRGLTPVQRQRLMARAFLAGVDILMISKVDFTGAWGYFQE
                     VYANMLPAAEQQALLAATGIASWDELRAKFKARITESAARIRSVKAKVGLSTSFMKTG
                     PATAASTDLVGEYRRLAN"
     gene            2095..3111
                     /locus_tag="VMZ53_09765"
     CDS             2095..3111
                     /locus_tag="VMZ53_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28788.1"
                     /translation="MPVDAKWDVPKDYDLIEREVVLPPPRGSLGAQPLLGILSVRALE
                     DQAAGEGVLSLVAWQTLAGATTNALQTIERIANKMEMGGLELLMLGHAYVATGLGEGA
                     GFEVAQRARKILKQPVTPYSIDASLLLAVTALPYGAERVRTLLEMLEPELQHGTDEHR
                     AVHALLRAAADPDQPARLDDAFTFFATLDDQLGLAQCALVAYAREAATTKRAADMRAY
                     LEHAIFRYDADGRPEWASRAIVQLLIPLLVDEIKAPPDVIIEQLARAAGFAVQAKSQL
                     AIEAVFRTAGKLGYAATVTALSAFDPPTFAKRDAAAAPPAEAPAAKPKKTATKKPSKK
                     KSAS"
     gene            complement(3108..3629)
                     /locus_tag="VMZ53_09770"
     CDS             complement(3108..3629)
                     /locus_tag="VMZ53_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28789.1"
                     /translation="MIVGCGGDDTTGPLPGGGGNVADSIGAVVTMEDDSALHIVIAST
                     SNLCSDARANPPIDRKGQSFVVIELADTGGAATTAPSAPGTYTVYSNSGSRPVKSASF
                     ISGAFDNACMLDDAADASGQSGSIVLTAIGARFAGTYDVVLNTGDHVTGSFDAAACTE
                     LQTATTTSQHACN"
     gene            3718..4593
                     /locus_tag="VMZ53_09775"
     CDS             3718..4593
                     /locus_tag="VMZ53_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS28790.1"
                     /translation="MGLRVLAVVLVLAGVAHADPNADVTQAFGAFIDGVATGKGSLTG
                     VDVFVSPLSDETDDDGVAARVPSDLADVKALIASSKLKVTKVVVSKGGKSAWIAGEVS
                     GKVERKGKKKTEPIRVSAFLGKDDKGWHVQATHWSVGEKDTKTEMCGSLEPWRVAANV
                     PKDADATVKAVYAALEGDWLMEGGVKTSKFGKLLSDDKNAVAIGSAPKEVFTGGAKIK
                     SVFKKWQISANLDEGKPQARAGIGPDGEMMWIAMSVTAPPDLCTAYRTLFVLAKESSG
                     WRIVHHHYSQRLNPY"
     gene            complement(4590..5684)
                     /locus_tag="VMZ53_09780"
     CDS             complement(4590..5684)
                     /locus_tag="VMZ53_09780"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HUS28791.1"
                     /translation="MKQLACFGVLAMLIACGGSDEGVGGSPPALGTTPKTWEYVPIPG
                     AQCMNGTPTGIGVNLGTSGEVVFYMEGGGACFNTETCKHVAHASGWGADQFAVNVGPY
                     NIGIFDRLDDANPFRDATFIFVPYCTGDVHSGSRPDGIDGRKFVGYQNVGLYLDYVIS
                     KSTDVRRVILAGSSAGGFGALLNYDRTQTAWGDVPVYLIDDSGPPLGDAFLAPCLQQL
                     FRDAFNLDAAIPADCAACKQADGGGMLNALGYLADKYPDRRMALVTSLRDGVIRSFYG
                     YGYPNCVAGAEGFPMPEEVYEQGIVDLRDRALASHPNFRVYSKDSGEHVWLLFEVETI
                     SPKRDGSGKHLSDWLHEMLDPDSNWQSVAP"
     gene            5708..6361
                     /locus_tag="VMZ53_09785"
     CDS             5708..6361
                     /locus_tag="VMZ53_09785"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HUS28792.1"
                     /translation="MLSGWEPDRLQHPRFARVADLVTRLTSERDWPEVATLNAAFAPE
                     LGSVGVRLVEAPKLKPALGSDGAIDIASLYEVRIVEAGEIPTRPRNAHDLLNALVWAA
                     FPRTKLALTRELAAVQRVRAEGRATLPNARSPAHDKLAMFDEGALVRVRGTTWIFGHA
                     VYEHAYAGVFDVRGAAVELDLEIDGLSTIDARARIDGALAAADLASLVRTGPGVPVD"
     gene            complement(6358..6720)
                     /locus_tag="VMZ53_09790"
     CDS             complement(6358..6720)
                     /locus_tag="VMZ53_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HUS28793.1"
                     /translation="MSERGACHCGAVTIEVATPPETVTSCNCSICHKLGTLWAYYAPA
                     DVTITGRTTTYQRGDKTIDFHHCSTCGCTTHWSPIPAIREANRMGINARLLAPAVLAA
                     ARVRRLDGASEDWKYLDD"
     gene            complement(6735..7349)
                     /locus_tag="VMZ53_09795"
     CDS             complement(6735..7349)
                     /locus_tag="VMZ53_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28794.1"
                     /translation="MFVRLERLLSRLRQPASRADELIALRHRVRDELASKQVDDEERA
                     LARDVHAKKLAVSAALQGVSSCASCAKGQPSPVGDYAGGACCSGVTADLFDSPELAAL
                     VHAGTRVADLAPPSRTDHHAGCAFRGAHGCSLDVEARPSRCVHYVCDGLRHELHDRGQ
                     LDDIEAKLAELNAAMRAFTVVHNARRDREVLAPLIAALENATRR"
     gene            complement(7350..8423)
                     /locus_tag="VMZ53_09800"
     CDS             complement(7350..8423)
                     /locus_tag="VMZ53_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUS28795.1"
                     /translation="MRRRFQTALLVYTAAHLYIWWRLIWPLPSPWWQIATVALPFIAA
                     TFPVGMVRTRRLPMPRQVRQRWLLPGYLWFGFATYLLLGAIASHIAVAFGTEAVRAAQ
                     ICSAVSIVVVIAGLLNVARGPTVKRVRIPMRGLPVEQYTVVHLTDVHIGSLIGKDFAE
                     QLVERVNALRADLIVITGDLVDGNLAELRPHIDPLRGLRARDGVYSVTGNHEYYWNVE
                     PWMLHLRSLGIEILRNERRTIANTLELAGTDDSSATEDVPRAVAGRDPNLPLVLLAHH
                     PRTVKRAMAAGVDLQLSGHTHGGQMLPWGYLARLWDPKVSGLARFDRTWLYVSQGTGY
                     WGPPIRIGTSCEIAAITLVPEAG"
     gene            8472..8858
                     /locus_tag="VMZ53_09805"
     CDS             8472..8858
                     /locus_tag="VMZ53_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28796.1"
                     /translation="MATPPVCPSCGSLGVGDHCAQCGTKMKPLGKADEMLTVAAPGLQ
                     LDGVEDAKPALVTDKRHRAASAYLALQPSETAVLHAASRIFAGFVANGEVTDANEKEL
                     SDRAVRLATRMALVIEKYVQSDDEDW"
     gene            complement(8859..9041)
                     /locus_tag="VMZ53_09810"
     CDS             complement(8859..9041)
                     /locus_tag="VMZ53_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28797.1"
                     /translation="MLKLLANLFGRRSSRFNRFGFGRQPLLTPRRGGIGLGTLAAVAA
                     PYIIRKVMQRRALNAG"
     gene            9117..10640
                     /locus_tag="VMZ53_09815"
     CDS             9117..10640
                     /locus_tag="VMZ53_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28798.1"
                     /translation="MRWPLLLLAACSAPAAAPAPASPVKRAAELPMSEPAEYDIDRIS
                     RAAGEDIFVRTGIGDPYRTGVPYPIFVALMRVFPETFGKDTHELSQKFGFVERAPDAA
                     SSDPDVRAGLPIGMHLTIDPITRVPFVVTSCAVCHVEQVRWQGGAATVVGLGNKRVRI
                     HDYDAAFSAITTRRELTADKMSRVAREAARDGAITWPDEYADALVAGTLSALRARAKE
                     REELVVKAAHGLPGRVATIEAFVPALRALSGKHVDYAHDTGWAKIPDVIGFRHRTTLS
                     WDASGEGPMALLLVEADIAVGARIEWFERHPFQGASLDAFLRQPAARPKFPRALDRAL
                     VEKGKALFEQHCADCHGTYAADGDAIDYDEPIVALADIGTDPVRASAATETFERVAND
                     RDLTRGYTRFRRTFGYVPPVLTNVWMRAPYGHAGQWPSLAVLAMPPDKRPTKYVVDTE
                     ALYDLDAVGIATHPAAPLQPGEYLYDAGKPGYSVAGHPFLADLGKDASAVIAYLESL"
     gene            10650..11138
                     /locus_tag="VMZ53_09820"
     CDS             10650..11138
                     /locus_tag="VMZ53_09820"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HUS28799.1"
                     /translation="MLRAGILSVLVTLAACGGGSKTTTAPTTPAKAAKILVNVDDSGV
                     GLGGNDPVAYSKANAAVAGDASMTSSFGGASYQFSSTDNKSAFDADPKRSAPQYGGYC
                     AFAASQNRLSESDPAIFTIHDGQLLVFTNEDFREQFMKDPAGNKAKADQNWPGLVEQY
                     GK"
     gene            11321..11503
                     /locus_tag="VMZ53_09825"
     CDS             11321..11503
                     /locus_tag="VMZ53_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28800.1"
                     /translation="MTRSFRARRITQPIAVIRRRRDLQLGTRSFTCFRLVPDGGALHP
                     IELVHSTARMFAFTEA"
     gene            11534..12109
                     /locus_tag="VMZ53_09830"
     CDS             11534..12109
                     /locus_tag="VMZ53_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28801.1"
                     /translation="MHVRTFAAMLVMIAACDAADDGVMPDAPSIDAPLPDSDPFVCMT
                     TARTAGPACLALTPSHLGGVTPFGNLDVDLAYVGAGDCISHSRATIQWIGACQEELVL
                     QFPYPTMSDSTAKRFVTGSFDPTWATVELRAYGEPPRRRDSSVHVDVTDWREGDGVHS
                     IDITVTFTDPGFAIAPMHIAGTFCDWPYYLC"
     gene            complement(12106..12471)
                     /locus_tag="VMZ53_09835"
     CDS             complement(12106..12471)
                     /locus_tag="VMZ53_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28802.1"
                     /translation="MTDSPTVRARLPYASLAGIVLVVLMTLGNAAANMIPTHLPTTRE
                     LQRAEQDEIVQARRDRIAELQAAGDHCDAQIARELSRSLFFDGRSAAPYADDYASRCG
                     DDPVVRRWADASAKLHLRR"
     gene            complement(12539..12703)
                     /locus_tag="VMZ53_09840"
     CDS             complement(12539..12703)
                     /locus_tag="VMZ53_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28803.1"
                     /translation="MRTIQISIGELIEAVYEELVEQYGDRELALVAAQAVGDDLLQRL
                     APPAQQPSGN"
     gene            complement(12822..13145)
                     /locus_tag="VMZ53_09845"
     CDS             complement(12822..13145)
                     /locus_tag="VMZ53_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28804.1"
                     /translation="MDPGTLEVTVDYPAVAFFYFLMTPNIVIDGQLERRRWGTHAFEV
                     AAGRHKVEVSYPWFLIRRAGKRAVVVDVLPGQTVRIHYRANVIRYVPGTISVDAQIPE
                     ARALE"
     gene            complement(13161..13724)
                     /gene="def"
                     /locus_tag="VMZ53_09850"
     CDS             complement(13161..13724)
                     /gene="def"
                     /locus_tag="VMZ53_09850"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HUS28805.1"
                     /translation="MMTLEIVQVGHPVLRQEARELSLEELASPRIQKLIEDMRDTMRA
                     APGVGLAAPQIGESIQLVVIEDPPAYHTKLTAEELAAREREVVPFHVLVNPTLTIRSE
                     DIVTAFEGCLSFAGFSMMVPRARKVRVEALDENGQRVVKVATGWYARILQHEVDHLRG
                     TVCIDRAETRTLTTTDNYVKYHSPNRT"
     gene            complement(13724..14440)
                     /locus_tag="VMZ53_09855"
     CDS             complement(13724..14440)
                     /locus_tag="VMZ53_09855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS28806.1"
                     /translation="MFARGGPTFRELMQQAFASTQRGYDLLAPKFDKTPFRTPDAIVE
                     TMRDLLGAPGSVGSALDVCCGTGAALRQLRPLARERVVGIDFSEGMLEEARQNLATAP
                     GDARVELVHGDALAMPFENEFDVATCVGALGHIEPKNEDRFVAGISRALRPGGRFAFA
                     TTRRPSPRELAFWLSHGFNAAMRVRNAIIKPEFVMYYLTFVWPEVQELLARHGLRAEA
                     FEGRCPPPFQNAIIVVATKR"
     gene            14464..15177
                     /locus_tag="VMZ53_09860"
     CDS             14464..15177
                     /locus_tag="VMZ53_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28807.1"
                     /translation="MEDVEPFIEWLRGQGLPAEHIAAYREHLAELAKHPSLSAAIRAA
                     EEAGTPAKQIANMRQVAARLVEFNEKGAVATARGTPAASVDIARGTPALEVEPRTRLK
                     PITSRLAHIDPPRRGCECKKRYDVYLDNDFGALARWLGGGIGIGTLILIRLIGLLGAA
                     ALALGMAGMGGFVTCFTICVRCEGCRRRISDLDDEELRDLRKGRGLVVLVTAGLLAGA
                     ALCGYLWISVMTSRVHRHY"
     gene            complement(15174..16043)
                     /locus_tag="VMZ53_09865"
     CDS             complement(15174..16043)
                     /locus_tag="VMZ53_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28808.1"
                     /translation="MRAIIATLGAWFIYGLIWIFGRTHRRADIPWLDGPLGDRVIGDA
                     PFQSVAEREGLSVERKAHSGGLVPSFEMLRGGDFDPAKVHPLVRNFYENTTDFTFDVW
                     SRTYFPANIGLFLLVTTISRQVNQLNFPLSPLDTATGMTSEIILMREPEGRVKYTGWF
                     RTLAKQELVLYTGFYMTQAVPGESGPCVKVVFPMPNGNATVILRPRVDETGALSLDSS
                     GGKFGGAGFYRVQGRSNGRVRVWKIRTLREHFRVFVDEAGVARCDHAVSFLGLPVLRL
                     HYKMLRRPAQAAA"
     gene            complement(16040..16558)
                     /locus_tag="VMZ53_09870"
     CDS             complement(16040..16558)
                     /locus_tag="VMZ53_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28809.1"
                     /translation="MRRLAVLLIAACSSPAPPAEHPTETPPATPEPRQDAAAEPDAGP
                     PQAVLDAPAWVFRYSTKDRAETWTLRHAEGSALLVVETAQGAQRYTGTATDGETLKLD
                     VSTGTAKMTLDCKRAKRPLSAKCNDQKAKPIEVLECFHPDFKTPMPFGPAPGVEYAVD
                     ASCNGYRLVPKP"
     gene            complement(16572..17240)
                     /locus_tag="VMZ53_09875"
     CDS             complement(16572..17240)
                     /locus_tag="VMZ53_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28810.1"
                     /translation="MLRASLLVVVALTGCMRIYPDPELPDVEVEWYEGDCDQPGIGDV
                     SIELTGIDDPTAHHNVTVACGPAKTVIKDVSRQRFKVVGQLLMTSGEVYSAAEYEVDL
                     RNGFDQTAYLYFGSFDTFRVAWTFDMGATCASVGADGVSVMFSMNDEPFFATDERCEL
                     GALTGSAPSGTYSISAIAYNFDSGAVLATSLAPVPDVTIQAGMRVDIGTIVLTPCGSS
                     CPDL"
     gene            complement(17227..19464)
                     /locus_tag="VMZ53_09880"
     CDS             complement(17227..19464)
                     /locus_tag="VMZ53_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS28811.1"
                     /translation="MRWVPAIACVAALTRVAAADTEVRGRVVDDETGSPIAGASVLLG
                     DQAAVTDDSGDFTVDGEGTLTVTAAGYAAVTVAAKPGLQIRLSKKTGEVIEISGKAPE
                     EAKPIAYVMTAKDIASTPGAMNDALRAITILPAASRIPFSFGGVVLRGMSPRDSSVFI
                     DGVEIPLAFHFGGITGVFPTQALDNMRVVPSGFDVSMGRTQGGVVELTTRRPRGDAYR
                     VGGEVSLLHSLVSAEGPVPGNGAALLTLRRSYFDFFVRPLVTRNDPLPSYSDGVLRAV
                     WGEPAKKGELAAYLFGSLDRIANSSDAAKPMDPDADGHIAANLGFVRTGVTYKRKIDK
                     TLVTLAPTLGTNILTLYEKDYPNDTTTVPDVTDISRRWYLFGGRGEWLRDDDHGFLRA
                     GIDLEGGYLGRIKQTKAEDIDNDTPFPRNTVLWANAAIFAEARRHWYGDRLSVRPGLR
                     FERFGLGEQWAVDPRVNAHATLTSTSTLRASLGRFHQPPSPAHFDEFADNLKAKSSYV
                     DQATLAVELSPEEGVLASITGFYHEGRHTLVDIVDKNVRSSAIDLELIFKELLEEQIG
                     LYGFQGNVGRQRTYGIETSMRYDKPRYRFLANFTWSRAKRQYDPALKLGWVPYGLDQP
                     LRVNLLAATSARKWNFGARLTVASGNPLRWVPAGTPTTGLDQEPAPVLQRLPTFWQLD
                     VRVDHAWKQSWGSVIGFLDLQNVTNHRNVEFRSSYPDENGVYRIEDDRGLPIIPYIGV
                     EFAPR"
     gene            complement(19519..20115)
                     /locus_tag="VMZ53_09885"
     CDS             complement(19519..20115)
                     /locus_tag="VMZ53_09885"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS28812.1"
                     /translation="MKRGIVAAVLAAALGAVLVLARQPAHREQAPVHARTLTPIEHAS
                     EAGNVEYLAATVRAEGPSAAHAAAAIARIDDTAAAPALTAIARDLQAPVLLRANAIHA
                     LAACDGDGALLLELATNDREPMRVRQEAALALRSRGTPTAVAPLATALDHEPDEQMRI
                     SLIQALKAIDSADARAAVAHHAQRQLSATERAFVVAKS"
     gene            complement(20112..23771)
                     /locus_tag="VMZ53_09890"
     CDS             complement(20112..23771)
                     /locus_tag="VMZ53_09890"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS28813.1"
                     /translation="MRSTLFAVTFVACLGGIAVDASAANVTIDARPLSTNQPIFISGP
                     RSLSFSGTVTADLPAGNYTLGELSWGTAGQTVNRVAFSVAIDGTVNYDASLAGVFAGS
                     GTTTLKANGVLVNVTSQLSSQHQTITYVNLPNQTSYAIRVLPGIYRMGYQTHSSAGYN
                     ECAFTFNVGADGQTSVATGASAYVGTGQNLLDVRGYTLNVQSSIIGYSHGVNLLTDPG
                     VPSYSLHVLPGPTEYFVFTENGNDYPYTANAFDFSVTSDGKVTYSPTVETYVSGAGTS
                     TLIVEGFSIRFETPLVGYNHGVAQISGQPSSTLTVSLLPGVYKYYVTNYTLAKQYGAN
                     FFIFYVSPTGTVTYDPSLTYLRGANTSTLYVDGVKLHITTPLVGYNHGINLIAGQTRT
                     DEDVYVLPGAYSYYVTTFVGGQYLANEVDFSVGTSGAVDYAATFDGWLHGRGTSTLNV
                     AGYLIRFDMHLAPNTTAYDVALIQGWSPKSVIGQTYLIPGKYTLEAGALSFRFDVSQS
                     GALDYAASLDGTIYGRGTSYMTYGIPACTTNNDCAGGLVCNSGLCGADTLAPTISIVS
                     RADYTSDATTTLVGRVTDNGRVASVQVNHATVAVAADGTFEAELSLVEGSNSIEIVAA
                     DAAGNTNALSTQTTRDSVAPEIVVLSPLSGQLIGDDGLVAVTVRITDASPTTVTIDGQ
                     PAVVPADGTVRVVASVPANGLNSIAIVATDAADNTADASATVLVDRQAPQLVSDLVDG
                     VSRGPLPGDIVTFNLFVNDVGATTVSLSDGSSYHLPRGGGVIAVAYLLHEGDNAIRAT
                     VENEGHHVAMFSSMQRYDRSAPTLALVSPAEGSVYRGWLELSADSVDAITGVETVAFQ
                     LDGGATLAAAASGAAWTVAVDTRTLQDGTHTVRATSTDRVGNQATANATFVVDNAAPA
                     VSLLGPNEGAYLRGTVTLVAQAQDATSGVAQLDILVNGAVVRSCASGSCSVPFDTTSV
                     ASGPLSVSAVATDRAGNVSAQAQVHAIADNDAITSFLTSPSPGAVVKTSLLVAINVRD
                     ADFASASCSVGSTALGGTTDPQFQQTVDLTSQLDGALTVSCSATDAAGNVGTESATVR
                     VANWTEMFHPQSLRLPAKGNWVTMSVRGVNAAILVPINARNLTLRVPGGAAVAASLGR
                     VHVAGDKTSFDTEVDECDGPSATHVDLRFNRAAFSASVKAGIAAGLINPNQPVTVTLY
                     AGDHLIGSDSIKVKP"
     gene            23992..24594
                     /locus_tag="VMZ53_09895"
     CDS             23992..24594
                     /locus_tag="VMZ53_09895"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HUS28814.1"
                     /translation="MSDDLLLHGSILLVFAWLAIGSLGAPLPEDAALLAAGVLVQRGA
                     VHPLVAVVVVLIGVLGGDCVLFFLGRRLGPAAYKRKLIQKALPPHRREKIERAYEKYG
                     GRLVFLARHVAGLRAAVFAMAGIHGMKPRRFLAWDAAAACISIPFVMGLGYVGAKNVD
                     RMRAGIAEARHYLLVIVGVAVLGFITWRHLRLRSAAKLEG"
     gene            complement(24591..25121)
                     /locus_tag="VMZ53_09900"
     CDS             complement(24591..25121)
                     /locus_tag="VMZ53_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUS28815.1"
                     /translation="MGLLRKLFGKDTTTDVLELLTSQHTEVDDLFARIEKRDGNVRAL
                     FTQLADKLAAHATVEEKIFYPAIMAKGTEDKLREAVEEHLSMKRLLADLIALSPDDDA
                     FDAKLKVLKEQVGHHAHKEEEAKLFPQVKDMLSADERAALGNELLVMFEELMLSHPYK
                     NLPAETRTAARLPSVR"
     gene            25175..25831
                     /locus_tag="VMZ53_09905"
     CDS             25175..25831
                     /locus_tag="VMZ53_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28816.1"
                     /translation="MWARVHKVDKIKALPGGGAVVVLEDERNASHMQRVPSLSSLIAI
                     ARILAAKRLLAIKFEGKGEIRYATNALVPSFLSETIARAGAALTERDGEKILMPAAPS
                     GVSALIDVGFSELAHHARGTVGIVDIGAALKHIEQKRRASPVDRDAKPEVYWPAVFEL
                     AALAGEQSRARGGRWVETTELPVPFALKFPEGQIAKPTEMAQKIVEGKDELDASMADV
                     "
     gene            complement(25828..26514)
                     /locus_tag="VMZ53_09910"
     CDS             complement(25828..26514)
                     /locus_tag="VMZ53_09910"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HUS28817.1"
                     /translation="MKHIGIVGCSAEGAALCYRTICSEAARLTGSHEHPEITMHTPSL
                     ALYVDRLERGDWAGVGELMLASADKLAAAGADFLICPDNTIHQALPLIVERSPTPWLH
                     IAEVVADAARARSYKRIGIMGTRWLVASDIYPNALASRDMTAVTPSSGDADLMNRIIM
                     DELVPSIVRTESVRALEAVIDRLRDAGCDAVVLGCTELPLVLGPANSTLPTLDSTRLL
                     AIAALQRARA"
     gene            complement(26511..27878)
                     /locus_tag="VMZ53_09915"
     CDS             complement(26511..27878)
                     /locus_tag="VMZ53_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HUS28818.1"
                     /translation="MATTARSNGVAAMSAGVVLRGLRKIQPRRDLPPGPQSPPLVQLA
                     RWVLRPIQLLEECQREFGDVFTWGGFREGDFVIVADPDMIKQVFSADPETLLAGVGNA
                     VLLEPLLGKNSLLTLDRAEHLRQRRLLLPSFHGERMQAYSSTMRAITSEAVDRWPVGK
                     DFALHSEMQAITLDVILRTVFGIDQASKHGGLRAQLIDLLEILSNPWLMFPGLLGFNP
                     FTVPWLRVAKLKKSVDDLLYRIIAERRAAPRGTDVLSMMLDAKDDKGEPMSDVEIRDE
                     LVTLLLAGHETTATSLAWVFDQLLTHPAVMTRLREDVAAGKDEYLDAVIRETLRIRPI
                     IPLVGRVVAKPFQMGPWHLPVGTRIAPSIYLSGMRAASHPEPKRFNPDRWLGVKPDPY
                     TWLPFGGGIRRCIGMAFAQLEMKIVVQTIVQRTRMRAVGGEASVVRRGITLAPSEGRR
                     VVIGA"
     gene            complement(27949..28644)
                     /locus_tag="VMZ53_09920"
     CDS             complement(27949..28644)
                     /locus_tag="VMZ53_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28819.1"
                     /translation="MSAKRILDRVHSRASITALELQPAEYARLVREEVLAGEHAGTAI
                     TLADLEGRDATLAQAIADIDELAMRVMRIELDHTLADDTAIPTQTRKVFAATVVAYAS
                     NLSLLEDRVRDIAARGRAPDADMTARMAVDAAKRTLALREALRRPLLDLARDLAQSTT
                     EPADKRAKDRTLDESWRKKWSAARRELEAVAAQPGRIALAPWPSRLAAHPEQIDEPGP
                     EADASFADMIEMD"
     gene            complement(28654..29496)
                     /locus_tag="VMZ53_09925"
     CDS             complement(28654..29496)
                     /locus_tag="VMZ53_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUS28820.1"
                     /translation="MRIALLAMLFAVGCTSSRVVDHVGATPAKPQATLGMTYTLDSKI
                     LGEKRTINVYVPPDYSMSARYPVLYMPDGGMNEDFPHVVGSVDVSIKNAIIRPVIVVG
                     IQNTERRRDLVGPTTLEEEKKIAPHAGGADRFRQFIRDELKPFIAAHYAVSGESALVG
                     ESFAGLFVVETLLVDPTLFDGYISADPSVFWNEQSLVDNATMYLARFPADGRPRHLYV
                     ATADWEPTQQVIANLLTAMRQVAPPGLTWTYEPMPTEHHSTIFPTAALHGIRTLFAAP
                     PDAE"
     gene            29523..30818
                     /locus_tag="VMZ53_09930"
     CDS             29523..30818
                     /locus_tag="VMZ53_09930"
                     /inference="COORDINATES: protein motif:HMM:NF002461.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HUS28821.1"
                     /translation="MQVEPPRVGPKPPFYTTLYFRVLVAIAAGIVLGVAYPTFAAKLQ
                     PLGTGFIKLIKMTIAPLIFCTIVVGIASMKSMKSVGKVGGLALLYFEAVTTLALVIGL
                     VIVNVIKPGSGMNIDPHGDPSAVAKYAAPGQMKGTVQFFLDIIPDTFVGAFVHGELLQ
                     VLLIALLFGFALHGVGERGKPVLDLVDRLGQVIFKIVGLIMHLAPIGAFGAMAYTVGT
                     LGLHSLLKLGELMACFYGTCLLFIFGVLGTIARIHGFSIIKFLRYIGDELFIVLGTSS
                     SESVLPRMIGKLERLGAGQGTVGLVVPAGYSFNLDGTSIYLTMGAMFVAQATNTHLSL
                     VQQLELLGILLLTSKGAAGVTGSGLVVLAATLGVVGHVPIVGIAAIVGIDRFMSEARA
                     LTNVIGNGVATVVVAKWVGDLDSQKLSAELASGPPPTEA"
     gene            30978..31805
                     /locus_tag="VMZ53_09935"
     CDS             30978..31805
                     /locus_tag="VMZ53_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28822.1"
                     /translation="MLQVLGMGIGLAAVDTDDTGNLKGIGIGTGVWSLIAPLIAMFLG
                     AMLVGRLCGSRDRKIGAMHGSVMWALALIIGMWAMVSVVSSIAHGVARVGGAAIGATG
                     SVVSSGVGAASRNGDGAMSALGIDTNDLLGPINQRLKENGKPPITANQLEATTRAVAQ
                     RGLHQGKLDRNVLVDEIAKNTSLSRADAEDLANQFEDKYQAMADKAGNAVDQVSEKAK
                     DVALETADKTGKVLLFGGLMMLLSLGAAIGGGMLGVKMREREEASDRTVVPPMSGGL"
     gene            31824..32468
                     /locus_tag="VMZ53_09940"
     CDS             31824..32468
                     /locus_tag="VMZ53_09940"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28823.1"
                     /translation="MDPEFWRGKWAERQIAFHEGKPNNFLSKHVTRLTGRVLVPLCGK
                     AEDLAYLASVGHEVVGIELVESAVAEFFSEHDILPTVTRVEGFAVYVAGGITLIAGDL
                     FATTRELIGRVDSIYDRGAMVALPPDMRRRYVHQLRQIAPAAQRVLLVTVDYPADAMN
                     GPPFSVPEAEVRSQYANAPIDVIDQGADPRGRLDGQMMERCYSIDLLTSAKPTS"
     gene            complement(32443..33744)
                     /locus_tag="VMZ53_09945"
     CDS             complement(32443..33744)
                     /locus_tag="VMZ53_09945"
                     /inference="COORDINATES: protein motif:HMM:NF027993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28824.1"
                     /translation="MKNAILGISLALVGSVSLVGCVDGADDEECLPGDNDCNPTPVSD
                     GKADGFDYANDPKLMANHLEYKLEKLPKKGTREKPVWKDTYPEAVGKAATVWADTYWP
                     ASEGSHNARWQGATVKSPLEKYDAAFNGKPGCDVQPEVSGRDSKAKWATYNQCAGPAA
                     KWQTEEYESIGLMHNGIDDDHDGNIDTYGSDGIDGVQGWWGTCHAWTPASQMIPEPQH
                     AVTVNGVKFDVGDIKALIQNVFDQTDAVMLGGRCNAKEITHDVAGSANDECSDVNPGA
                     LHVVMTNFLGLRQLPLVEDRTANYEVWNQPVVGYEITKQDKVDAKTAMKCVGATGDKW
                     TYNTKAKSLYEVRMNVKYVSESSASNTPHGFMNNVSDDDYHYILELNGYGKVIGGRYC
                     SDGENSHIDFLWSPTGSFSPSNPYVDVAKVKDLIKKSVSPM"
     gene            33915..>34354
                     /locus_tag="VMZ53_09950"
     CDS             33915..>34354
                     /locus_tag="VMZ53_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28825.1"
                     /translation="MKPRYTVLERLGGGGQAEVFKGISESMQGFKKSVAIKRVLPNLT
                     SNEQFVAMFLDEARLSLFLQHANIVQVFDISKADDGTYFLVMEFVDGCDLKALISHFG
                     GGGKDLDIGLTLYIMIEAAKGLNYAHQLENPQTGQKLNIVHRDVS"
BASE COUNT         5881 a        11026 c        11485 g         5962 t
ORIGIN      
        1 acgctcctcg gcggtggcga cgcgttcggc gacgacgact cgatcttcag cggcttcgcg
       61 ctctcgcacg acggtgcgac tgcgttcatc ggcgacagca atgtggtcgg cacgaatcgc
      121 gtcggcatcg tgagcgtgag cgcgaccgac atcgcaccgc tcgcggtgat cacgacaaac
      181 atcacggatc cgagcggcat cgcagcgtcg ccgttcggcg atgtcgcgat cgtgacgagc
      241 tcgcagccgc cgggcgaagg catctacgtg ctcgacaagg gcggcacgaa cggcgcgtgg
      301 cgcaatcgcg gcgagctcac gtacatgggc gccgcttcgc agctgcccgg cgatgtcgtc
      361 acgatcgatc gcgggatgct cgaaggcagc gtgatcgtct cggagctctc gaaggttcga
      421 cgtctgcaat ttcacgccga cggcagcgtc accgacactg gatcgctcgc gtttggcagt
      481 ggcctcgaag agatcgccgg cgcgattggc gtcacgccgt agtcctgaac gcgccaacgc
      541 gtgcacatcg agtgaatctg cacagttgct gtcgccggca acgcgagtag ccagcgtcgc
      601 gtcgtgatcg ccaagtaggc gaagcggctg tgcccgcaca cggcattgca gttgctcctc
      661 tcgcgagcac atgcggaatg ccgccatcgc ctcgctgctc gccgtcgtcg ccagctgctc
      721 tgcccaggac gacgagtacg tgccgaccgg cgcgcccgac gagacggact cgaccggcga
      781 aggcggcaag gaagacagcc cggcgcccgg gcctgaggtt ccggtcgctc agcagtcggc
      841 cgacatcggc cagttcttca tggtcgatca ctacggcgtc gacgcggcag gcttcgcgga
      901 catccgcaac aagatcaaga cgaagaacct cggcgcgatc atcctgtgga atccaacgaa
      961 cgcatcgggc gagacactgc gccagatgat ccgcggctac tccgacgtcg cgaaggccgc
     1021 gggccgcgac gagctgttct acgcagcgga tcaagaagag ctgcgcacgc agcgattcgc
     1081 gtcggcgaac ggcttcacat cgctcgtgca gggcagcgtg ctcggctcgg tcagcagtcg
     1141 cgatcgtctg tgcgagctgc acgcccgcat cacgtcccgc gagatggcgt cgaccggtat
     1201 gaacatgtcg ctcggcacgg tgtcggacat ctacacgagc gattcaggaa cgcgcggcat
     1261 gttccggacg cgcgcgatcg acgatcagcc cgagaacgtc gcttcgtgcg tcgtcgcgat
     1321 gacaaaggcg tacgcgcaag agggccacgt cgtttacatc acgaagcact tcccgggcct
     1381 cggcaacgcg agcggcaaca cggacgtcga tgcatcggtc gcgacgcggt cgaagaccaa
     1441 ggcggcgatg gagcgcgagc tcgcaccgta tcgcgcggcg tcggcgtccg tcaccgcggc
     1501 gaacggctgg ccgttcttcg gcgcgatgat cagtcacgcg agctaccccg tcatcgacgg
     1561 tggcgcactc cccgcgacgc tctcctccaa gatcgtcaac gacttcatgc gcggcacggg
     1621 caacctcacg ctcggcggca aagacagcgc aggcgccgcg acgacgttcg ccggcatggg
     1681 ctttcgcggc ctctccatca gcgatgcatt ctggacgtgg ggcgcaacgc gcggcctcac
     1741 gccggtgcaa cgccagcgct tgatggcgcg cgcattcctc gcgggcgtcg acatcctgat
     1801 gatctcgaag gtcgacttca ccggcgcgtg gggctacttc caagaggtct acgcaaacat
     1861 gctccccgcg gctgaacagc aagcgctgct cgcggcaacg ggcatcgcgt cgtgggacga
     1921 gcttcgcgcg aagttcaagg cgcgcatcac cgagtccgcg gcgcgcattc gctcggtgaa
     1981 ggccaaagtc ggcctctcca cgagcttcat gaagacaggc ccggcgaccg ccgcatcgac
     2041 cgatctagtc ggcgaatatc ggcggctcgc gaactgagct gatacgttca tcgcgtgcca
     2101 gttgacgcga agtgggatgt accgaaggac tacgatctga tcgagcgcga ggtcgtgttg
     2161 cctccaccgc gcggctcgct cggtgcgcaa ccgctcctcg gcatcctctc cgtgcgcgcc
     2221 ctcgaagatc aggcggcagg cgagggcgtg ctgtcgctcg tcgcgtggca gacgctcgcc
     2281 ggcgcgacga caaacgcgct gcaaacgatc gagcgaatcg cgaacaagat ggagatgggc
     2341 ggcctcgagc tgctgatgct cggccacgcg tacgtcgcga ccggcctcgg cgaaggcgcg
     2401 ggcttcgaag tcgcacagcg cgcacgcaag atcctcaagc agccggtcac gccctactcg
     2461 atcgatgcca gcctcctcct cgcggtgacc gcgctaccgt acggcgccga gcgtgtccgc
     2521 accctcctcg agatgctcga gcccgagctg cagcacggca ccgacgagca tcgcgcggtc
     2581 cacgctctgc tccgcgccgc cgccgatccg gaccagcccg ctcgcctcga cgacgcgttc
     2641 acgttcttcg ccaccctcga cgatcagctc ggcctcgcgc aatgcgctct cgtcgcgtac
     2701 gcccgcgaag ctgccacgac gaagcgcgcc gccgacatgc gcgcgtatct cgagcacgcg
     2761 atctttcgct acgacgccga cggccgccca gagtgggcct cgcgcgcgat cgtgcagctc
     2821 ctgatcccgc tgctcgtcga cgagatcaag gcgccgcccg acgtgatcat cgagcagctc
     2881 gcacgcgcgg cgggcttcgc cgtccaggcc aaatcgcagc tcgccatcga agccgtgttc
     2941 cgcacagccg gcaagctcgg ctacgccgcg acggtcaccg cgctcagcgc gttcgatccg
     3001 ccgacgttcg cgaagcgcga cgccgccgct gcgcctcccg ccgaagctcc ggccgcgaag
     3061 ccgaagaaga ccgcgaccaa gaagccgagc aagaagaagt ccgcgagcta gttacacgcg
     3121 tgctgcgacg tcgtcgtcgc ggtctgcagc tccgtgcacg cagccgcatc gaacgatccg
     3181 gtgacgtggt ctcccgtatt gagcacgacg tcgtacgtac cggcgaagcg cgcgccgatc
     3241 gcggtcagca cgatcgagcc gctctgaccg ctcgcatcgg ccgcatcgtc gagcatgcac
     3301 gcgttgtcga acgcgccgct gataaacgac gcggacttca ccgggcgcga gccgctattc
     3361 gaatacaccg tgtacgtgcc cggcgcgctc ggtgcggtcg tagccgcgcc accggtgtcc
     3421 gccagctcga tcacgacgaa gctctgaccc ttgcgatcga tcggcgggtt tgcgcgagcg
     3481 tcgctgcaca agttgctcgt gctcgcgatc acgatgtgca aggcgctgtc gtcctccatc
     3541 gtcactaccg cgccgatcga atcggcgaca ttcccaccgc caccggggag tggaccggtc
     3601 gtgtcatccc caccgcaccc gacgatcatc aaccacatgg caagcgaagc tcgagtcatc
     3661 gcgtgacggg ctgcaagggc gataccagct cgtcacgagt tgtgggatcg ttgggggatg
     3721 gggctacgtg ttctggcggt tgtgctggtg cttgcgggtg tcgcgcatgc cgatccgaat
     3781 gccgacgtca cacaggcgtt cggcgcattc atcgacggcg tcgcgactgg caagggctcg
     3841 ctgacgggcg ttgatgtgtt tgtcagcccg ctgagcgacg agaccgacga cgatggcgtc
     3901 gccgcgcgcg ttccgtcgga tctcgccgac gtgaaggcgc tgatcgcgtc ttcgaagcta
     3961 aaggtcacga aggtcgtcgt ctcgaagggc ggcaagtcgg cgtggatcgc cggtgaggtg
     4021 agcggcaagg tcgagcgcaa ggggaagaag aagacggagc cgattcgcgt gagcgcgttt
     4081 ctcggcaagg acgacaaggg ctggcacgtt caggcgacgc actggtcggt cggcgagaag
     4141 gatacgaaga ccgagatgtg cggatcgctc gagccgtggc gcgtcgctgc gaatgttccc
     4201 aaggatgcgg atgccaccgt gaaggcggtg tacgcggcgc tcgagggaga ttggctcatg
     4261 gagggcggcg tgaagacctc gaagttcggc aagctgttgt ccgacgacaa gaacgcggtc
     4321 gcgatcggca gtgcgccgaa ggaagtgttc accggcggcg cgaagatcaa gagcgtgttc
     4381 aagaagtggc agatcagcgc gaatctcgac gagggcaagc ctcaggcgcg tgcgggcatc
     4441 ggtcccgatg gcgagatgat gtggatcgcg atgagcgtga cggcgccgcc cgacctgtgc
     4501 accgcgtatc gcacgctgtt cgtgctcgcg aaggaatcga gtggctggcg catcgtccac
     4561 caccactact cgcagcgctt gaatccgtat tagggcgcga cgctctgcca gttggagtcg
     4621 gggtccagca tctcgtggag ccagtcgctc aggtgcttgc cgctaccgtc gcgcttgggc
     4681 gagatggtct cgacttcgaa gagcagccac acgtgctcgc cgctgtcttt gctgtagacg
     4741 cggaagttcg gatgcgaggc gagcgcgcga tcgcggagat cgacgatgcc ttgctcgtag
     4801 acctcctccg gcatcgggaa gccttcggcg cccgcgacgc agttcggata gccgtagcca
     4861 tagaagctgc gaatgacgcc gtcgcgcaag ctcgtcacga gcgccatccg gcgatctgga
     4921 tacttgtcgg cgaggtagcc gagcgcgttc agcatgccgc cgccatcggc ttgcttacac
     4981 gccgcacagt cggcgggaat ggccgcgtcg aggttgaacg cgtcgcgaaa cagctgttgc
     5041 aggcacggcg ccaggaacgc atcgccgagc ggcggacccg agtcgtcgat gaggtacacc
     5101 ggcacgtcgc cccaggccgt ttgtgtccgg tcgtaattga gcagtgcgcc gaagcctccg
     5161 gcggacgagc cggcgaggat cacgcgtcga acatccgtcg atttcgagat gacgtaatcg
     5221 aggtagaggc caacgttctg gtacccgacg aacttgcgac cgtcgatacc atcgggcctc
     5281 gagccagagt gaacgtcacc ggtgcagtac ggcacgaaga tgaacgtcgc gtcgcggaat
     5341 gggtttgcgt cgtcgagccg atcgaagatg ccgatgttgt agggaccgac gttgacagcg
     5401 aattggtcgg ctccccagcc ggacgcgtgc gcgacgtgct tgcaggtttc ggtgttgaaa
     5461 cacgcgccgc cgccttccat gtagaagacg acttcgcccg atgtgccgag attgacgccg
     5521 atgcctgtcg gcgttccgtt catgcactga gcgcctggaa tcggcacgta ctcccacgtc
     5581 tttggcgtgg tgccgagcgc tggcggcgat ccgccgacac cctcgtcact gccgccgcac
     5641 gcgattagca tcgcgagaac cccgaaacag gcgagctgct tcatgcgcgg gactgtatac
     5701 cagggacgtg ttgagcggct gggagcctga tcgtctgcag catccgcggt tcgcgcgggt
     5761 cgccgatcta gtgacgaggc tcacctccga gcgcgattgg ccggaggtgg cgacgctaaa
     5821 tgccgccttt gcaccggagc ttggcagcgt cggggtgcgg ctcgtcgagg caccgaagct
     5881 gaagcccgcg ctcggtagcg acggggcgat cgatattgcg tcgctctacg aggtgaggat
     5941 cgtcgaggcc ggcgagattc cgacgcggcc gcgcaacgcg cacgacttgc tgaatgcgct
     6001 cgtgtgggcg gcattcccgc gcacgaagtt ggcgctgacg cgcgagctcg ctgcggtgca
     6061 acgcgtgagg gccgaagggc gcgcgacgtt gccgaatgcg cggtcgccgg cgcacgacaa
     6121 gctcgcgatg ttcgacgaag gcgcactcgt gcgggtgcgg ggaaccacgt ggatcttcgg
     6181 tcatgccgtc tacgagcatg cgtacgccgg tgtgttcgac gtgcgcggcg cggctgtcga
     6241 gctggacctc gagatcgacg ggctgtcgac gatcgacgca cgcgctcgca tcgacggtgc
     6301 gctggcagcc gcggatctgg catcgctcgt gcgcaccggg ccgggcgtgc ccgtcgatta
     6361 gtcgtcgagg tacttccagt cctcggatgc accgtcgagg cggcgaacac gcgcagcggc
     6421 gaggacggcg ggcgcgagca gccgcgcgtt gatgcccatg cggttcgctt cgcggatcgc
     6481 gggaatcggt gaccagtgcg tcgtgcagcc gcacgtcgag cagtgatgaa agtcgatggt
     6541 cttgtcgccg cgctggtaag tcgtcgttcg tccggtgatc gtcacgtcgg ccggcgcgta
     6601 gtacgcccac agcgtgccga gcttgtggca gatgctgcag ttgcacgacg tcaccgtctc
     6661 cggaggcgtc gcgacttcga tggtgacggc accgcagtgg caggctccgc gttcgctcat
     6721 cgctgcatcg tagcctagcg gcgcgtcgcg ttttcgagcg cggcgataag cggcgcgagc
     6781 acttcgcgat ctcgccgcgc gttgtgaacg acggtgaacg cgcgcatcgc ggcgttcagc
     6841 tcggcgagct tcgcctcgat gtcgtcgagc tggccgcggt cgtgcagctc gtgccgcaga
     6901 ccgtcacaga cgtagtgcac gcagcggctc ggccttgctt cgacatcgag cgaacaaccg
     6961 tgcgcgccac gaaacgcaca acccgcgtga tgatcggtcc gcgacggtgg cgcaagatcc
     7021 gcgacgcgcg tgcccgcgtg taccagcgca gcgagctcgg ggctgtcgaa tagatcggcg
     7081 gtgacgccgg agcaacacgc gccgcccgcg tagtcgccga ccggcgaggg ctgacccttc
     7141 gcgcacgatg cgcatgacga cacgccttgc agcgccgcgg acacggcgag cttcttcgcg
     7201 tggacgtcac gcgcgagcgc gcgttcttcg tcatcgactt gcttgctcgc gagctcgtcg
     7261 cgaacacggt ggcgaagcgc gatcagctcg tcggcgcgcg acgccggttg gcgcaagcgc
     7321 gaaagcagcc gttcaaggcg gacgaacatc tagcccgctt ccgggaccag cgtgatcgcg
     7381 gcgatctcgc acgacgtgcc gatgcgaatc ggcgggcccc agtagccggt accttggctg
     7441 acgtacagcc acgtgcgatc gaagcgcgcg aggcccgaga ctttgggatc ccacagccgc
     7501 gcgaggtagc cccacggaag catctgccca ccgtgcgtgt gaccggagag ctggaggtcg
     7561 acgcctgccg ccatcgcgcg cttcaccgtg cggggatgat gcgcgagcag cacgagcggc
     7621 aggttcggat cgcgacccgc aaccgcgcgc ggcacgtcct ccgtcgccga cgaatcatcg
     7681 gtgcccgcga gctcgagggt gttcgcgatc gtcctgcgtt cgttgcgcag gatctcgata
     7741 ccgagcgagc gcaggtgcag catccacggc tcgacgttcc agtagtactc gtgattgcca
     7801 gtgaccgagt agacgccgtc gcgcgcacgc agcccgcgca gtggatcgat gtgagggcgc
     7861 agctcggcaa gatttccgtc gacgaggtcg cccgtgatca cgatgaggtc cgcgcgcagc
     7921 gcgttcacgc gctcgacgag ctgctccgcg aagtccttgc cgatgagact gccgatgtga
     7981 acgtcggtga ggtgcacgac cgtgtactgc tcgacgggca ggccccgcat cgggatgcgg
     8041 acgcgcttca ccgtcggccc acgcgcgaca ttgagcagcc ccgcgatgac gaccacgatc
     8101 gagaccgcgc tgcagatctg cgcagcacgc accgcctcgg tgccgaatgc gacggcgatg
     8161 tgactcgcga tcgcgccgag caagagatag gtcgcgaagc cgaaccagag atagcccggc
     8221 aagagccagc gctggcgcac ctgacgtggc atgggcaacc ggcgcgtccg caccatgccg
     8281 accgggaacg tcgctgcgat gaacggcaac gcgaccgtcg cgatctgcca ccacggcgaa
     8341 ggcagcggcc agatgagccg ccaccagatg tagagatgcg ccgcggtgta gacgagaagc
     8401 gcggtctgga aacggcggcg cacgggggtc gagacgatct agcacgcggc ggcctcctgt
     8461 acgatcacga catggctaca ccgcctgtgt gtccaagctg cggctcgctc ggcgtcggcg
     8521 atcactgtgc gcaatgcggc acgaagatga agccgctggg caaggccgat gagatgctga
     8581 ccgtcgctgc gcccggcctc cagctcgacg gagtcgagga cgcgaaacca gcgctcgtca
     8641 ccgacaagcg gcatcgtgcg gcatcggcgt acttggcgct tcagccgagc gagacggccg
     8701 tgctgcacgc ggcgtcgcgg atctttgccg gctttgtcgc caatggcgaa gtgaccgacg
     8761 cgaacgagaa agagctctcg gatcgcgcgg tcaggctcgc gacgcgaatg gccctcgtca
     8821 tcgagaagta cgtccagagc gatgacgagg attggtagct agcccgcgtt gagtgcgcgg
     8881 cgctgcatca ccttgcggat gatgtacggc gcggcgaccg cagcgagcgt accgagcccg
     8941 atgccgccac ggcgcggcgt cagcagcggc tggcgaccga agccgaaacg gttgaaacgc
     9001 gaagaacgac gaccaaacag gtttgctaga agcttgagca tgcactgtgt cattgcaggc
     9061 agcgttcccg aggaggccag cactcggctt aggaatttca ctgttacgat gactccgtga
     9121 ggtggccgct cctcttgctc gctgcgtgct cggcgcccgc cgctgcgccc gccccggcgt
     9181 cgcccgtgaa gcgcgcggca gagctgccga tgtcggagcc cgccgagtac gacatcgatc
     9241 gaatctcgcg cgcagccggt gaggacatct tcgtgcgaac cggcatcggc gatccgtatc
     9301 ggaccggcgt gccgtacccg atcttcgtcg cactgatgcg cgtgttcccc gagacgttcg
     9361 gcaaggacac gcacgagcta tcgcagaagt tcggcttcgt cgaacgagcg cccgacgcgg
     9421 cgagcagcga tccggacgtc cgcgctgggt tgccgatcgg catgcatctg acgatcgatc
     9481 cgatcacgcg cgtgccgttc gtcgtcacga gctgcgcggt gtgtcacgtc gagcaggtgc
     9541 gctggcaggg cggtgcagca accgtcgtcg gtctcggcaa caagcgagtc cgcattcacg
     9601 actacgacgc cgcgttctcc gcgatcacga cgcgtcgcga gctcacggcc gacaagatga
     9661 gccgcgtcgc gcgcgaggcc gcacgagatg gcgcgatcac gtggcccgac gagtatgccg
     9721 atgcgctcgt tgctggaacg ctgtcggcat tgcgcgcgcg agcgaaggag cgcgaggagc
     9781 tcgtggtgaa agcagcgcat ggcttgcccg gtcgcgtggc gacgatcgaa gcattcgtgc
     9841 ccgcactgcg cgcgctgagc ggcaagcacg tcgactacgc gcacgacaca ggctgggcga
     9901 aaatccccga cgtgatcggc tttcgccatc gcacgacgct gtcgtgggat gcgagtggcg
     9961 agggaccgat ggcgctgctg ctcgtcgaag ccgatatcgc agtcggtgcg cgcatcgagt
    10021 ggttcgagcg tcatccgttc caaggcgcga gcctcgacgc attcctgcgc caacccgcgg
    10081 cgcggccgaa gtttcctcgc gcgctcgatc gcgctctcgt cgagaagggc aaggcgctgt
    10141 tcgagcaaca ctgcgccgac tgccacggca cgtatgccgc cgatggcgac gccatcgact
    10201 acgacgagcc gatcgtcgcg cttgccgata tcggcaccga ccccgtgcgt gcgagcgcgg
    10261 cgaccgaaac gttcgagcgc gtcgccaacg atcgcgatct gacgcgcggc tatacgcgct
    10321 ttcgccggac gttcggctac gtgccgccgg tgctcacgaa cgtgtggatg cgcgcgccgt
    10381 acggtcacgc cgggcagtgg ccgagcctcg cggtgctggc aatgccgccc gacaagcggc
    10441 cgacgaagta cgtcgtcgac accgaggccc tctacgatct cgatgccgtg ggcatcgcga
    10501 cccatccggc ggccccgctg cagccaggcg agtacctcta cgatgccggc aagccgggct
    10561 actccgttgc tggccatccg ttcctcgccg acctcggtaa ggacgcatcg gccgtgatcg
    10621 cgtacctcga gtcgctataa tccgccgcga tgctgagagc tgggatcctt tctgtcctcg
    10681 tcacgctggc ggcctgcggt ggaggctcga agaccacgac ggcgccgaca acgcctgcga
    10741 aggccgccaa gattctcgtc aacgtcgacg acagtggcgt cggtctcggc ggtaacgatc
    10801 cggtcgcgta tagcaaagcg aatgcggctg tcgcgggcga cgcgagcatg acgtcgagct
    10861 tcggcggcgc gagctatcag ttctcgtcga cggataacaa gtcggcgttc gatgccgatc
    10921 cgaaacgcag cgctccgcag tacggcggct actgcgcgtt cgctgcttcg cagaatcgcc
    10981 tgtccgaaag cgaccccgcg atcttcacga tccacgacgg ccagctgctc gtgttcacga
    11041 atgaggactt tcgtgagcag ttcatgaaag acccggctgg caacaaggcg aaagccgacc
    11101 agaactggcc gggcttggtc gagcagtacg gtaagtagct gcgacagggg cgacaggtga
    11161 gggctgactg ctgagggcag tcggcggcag tgaggcacgc ggccagctac ccgagttgcc
    11221 gttatggcag ccgccgggtc cggagcgaac gtgcgaaagc gtagttaggc ggcatttttc
    11281 actggacggc gatggcccgt ccgttgctct atccgccgcc atgactcgct cgtttcgcgc
    11341 tcgccggatc acccagccca tcgccgtcat ccggcgccgg cgcgatctcc agctcgggac
    11401 gcggtcgttc acctgctttc ggctcgtgcc ggatggcggt gcgctgcatc cgatcgagct
    11461 cgtccacagc accgctcgca tgtttgcgtt cacggaggcg tgaccttttc ggtgcgtgcc
    11521 tccactaggg ggcatgcacg ttcggacctt cgcggcgatg ctggtgatga tcgccgcctg
    11581 cgatgctgct gatgatggcg tgatgcccga tgccccgagc atcgatgctc cgctccccga
    11641 ttcggatcca ttcgtctgca tgacgactgc gcgcaccgcc gggcccgcgt gtctcgcttt
    11701 gacaccgagt catctcggcg gcgtcactcc gttcggcaat ctagacgtcg acctcgcgta
    11761 cgtcggcgcg ggagactgca tctcgcattc acgagcgacg attcagtgga taggcgcctg
    11821 ccaagaagag ctcgtgctgc agtttccgta tccgacgatg agcgactcga cggcgaagcg
    11881 cttcgtgacg ggctcgttcg atccaacgtg ggccaccgtc gagctgcgcg cgtacggcga
    11941 gccgccgcgg cgacgcgatt cgagcgtgca cgtcgacgtg accgactggc gcgaaggcga
    12001 cggcgtgcac tcgatcgaca tcaccgtgac gttcaccgat cccggattcg cgatcgcgcc
    12061 gatgcacatc gccggcacgt tctgcgactg gccgtactac ctttgctagc ggcgcaggtg
    12121 cagcttcgcg ctcgcatcgg cccaccgacg cacgacggga tcgtccccgc agcgcgacgc
    12181 gtagtcatcg gcatacggcg ccgccgagcg accatcgaag aacagtgagc gtgacagctc
    12241 gcgcgcgatt tgcgcatcgc agtgatcgcc agccgcttgt agctccgcga tccgatcgcg
    12301 acgtgcctgt acgatctcgt cttgctcggc gcgctgcagc tcgcgcgtag tcggcaagtg
    12361 agtcgggatc atgttcgcgg ctgcgttgcc gagcgtcatc aagacgacga gcacgatgcc
    12421 ggcgagcgag gcgtagggga ggcgagcgcg gacggttggc gagtcggtca tgtctccaag
    12481 agacgcgtcg aaacagccag ttgcggggac gtccctgcga cgtcgcagtg tgtctcggct
    12541 agttgccgga cggctgctgc gcgggcggtg cgagccgctg cagaagatcg tcgccgaccg
    12601 cctgcgccgc aaccaacgcg agctcgcgat cgccgtactg ctcgaccagc tcctcgtaca
    12661 cggcctcgat cagctctccg atggaaatct ggatggttcg catggcgaag cacttcatca
    12721 agtgccgtgc cagaaacgcg tttggatcat ttcgatcact tggagccacg agagagatcg
    12781 cctggcaggg acagtgatca cctgtgtggc gactctggtg atcactcgag cgctctcgcc
    12841 tcagggatct gcgcgtcgac ggagatcgtg ccgggcacgt agcggatcac gttggcgcgg
    12901 tagtgaatgc gaacggtttg acctggcagc acgtcgacga cgacggcccg cttacctgca
    12961 cgtcggatca ggaaccacgg atacgagacc tcgaccttgt gcctgccggc ggcgacctcg
    13021 aacgcgtgcg tgccccagcg gcgcctctcg agctgtccat cgatcacgat gttgggcgtc
    13081 atcaagaagt agaagaacgc gacggcggga tagtcgaccg tgacttcgag cgttcccggg
    13141 tccatgacga aagctagcag ctacgtgcgg tttggagagt ggtacttcac gtagttgtcg
    13201 gtcgtggtga gcgtgcgcgt ctctgcgcga tcgatgcaga cggtcccgcg caggtgatcg
    13261 acctcgtgct gcaggatgcg cgcgtaccac ccggtggcga ctttgacgac gcgctggcca
    13321 ttctcgtcga gcgcttcgac gcggaccttg cgagcgcgcg gcaccatcat gctgaagccc
    13381 gcgaagctca ggcagccctc gaacgcggtg acgatgtctt cgctgcgaat cgtgagcgtc
    13441 ggattgacga gcacgtgaaa cggcacgacc tcgcgctcgc gcgcggcaag ctcttcggcg
    13501 gttagcttcg tgtgatacgc gggcgggtct tcgatcacga cgagctggat cgactcgccg
    13561 atctgcggtg cagcgagacc gacgcccggc gccgcgcgca tcgtgtcgcg catgtcctcg
    13621 atgagctttt ggatgcgcgg cgacgcgagc tcctcgagcg agagctcgcg agcctcttgt
    13681 cgcagcaccg gatggccgac ctgaacgatc tcgagcgtca tcattacctc ttcgtcgcga
    13741 cgacgatgat cgcgttttga aacggcggcg ggcagcgacc ttcgaaggcc tcggcgcgaa
    13801 gcccatgccg tgcgagcagc tcctggactt cgggccacac gaacgtcagg tagtacatga
    13861 cgaactcggg cttgatgatc gcattgcgaa cgcgcatcgc ggcgttgaag ccgtgcgaga
    13921 gccaaaacgc gagctcgcgt gggctcggcc tgcgcgtcgt cgcgaatgca aagcggccgc
    13981 cggggcgcag cgcgcgcgag atgcctgcga cgaagcggtc ttcattcttc ggctcgatgt
    14041 ggccgagcgc tccgacacac gtcgcgacat cgaactcgtt ctcgaacggc atcgcgagcg
    14101 catcgccatg cacgagctcg acgcgcgcgt cgccgggtgc tgtcgccagg ttctggcgcg
    14161 cttcctcgag cattccctcg gagaaatcga tgccgacgac gcgctcgcgg gcgagcgggc
    14221 gcaactggcg gagcgctgcg ccggttccac agcagacgtc gagcgcggag ccgaccgagc
    14281 cgggcgcgcc gagcagatcg cgcatcgttt cgacgatcgc atcgggcgtg cggaacggcg
    14341 tcttgtcgaa cttcggcgcg agtaagtcgt agccgcgctg ggtcgacgcg aacgcctgct
    14401 gcatcagctc tcggaacgtg gggccgccgc gcgcgaacac ggctggtacg atacgctgga
    14461 gtcgtggagg acgtggagcc gtttatcgag tggttgcgag ggcagggctt gcccgccgag
    14521 cacatcgccg cgtatcgcga gcacctggcc gagctcgcta agcacccgag cctgtccgca
    14581 gcgattcgcg ccgccgagga agcgggcacg ccagcgaagc agatcgcgaa tatgcggcag
    14641 gtcgctgcgc ggctcgtcga gttcaacgag aagggcgcgg tcgcgactgc gcgcggaacg
    14701 cccgcagcat ccgtcgacat cgcgcgagga acgccggcgc tcgaagtgga accgcgaacg
    14761 aggctgaaac cgatcacttc gcggctcgcg cacatcgatc cgccgcgccg cggctgcgag
    14821 tgcaagaagc gctacgacgt gtatctcgac aacgacttcg gcgcgctggc acgttggctc
    14881 ggcggtggca tcggcatcgg tacgctcatc ctcattcgtc tcatcggact cctcggcgcc
    14941 gccgcactcg cgctcggcat ggccgggatg ggtggcttcg tcacgtgctt cacgatctgc
    15001 gtgcgctgcg aaggttgtcg caggcgcatc agcgatctcg acgacgaaga gcttcgcgat
    15061 ctacgcaaag gccgcggcct cgtcgtgctc gtcacggccg gactgctcgc gggtgcagcg
    15121 ctctgcggct acctgtggat cagcgtcatg acgtcacgcg tgcatcgtca ctactaggct
    15181 gccgcctgcg cgggacggcg cagcatcttg tagtgcaggc gcagcacggg caggcctaga
    15241 aagctcaccg cgtgatcgca ccgcgcgacg ccggcctcgt cgacgaacac gcggaagtgc
    15301 tcgcgcagcg ttcgaatctt ccacacgcgc acgcgaccgt tgctgcgacc ctgcacgcga
    15361 tagaacccgg cgccgccgaa cttgccgccg ctcgaatcga gtgacagcgc tccggtctcg
    15421 tcgacgcgcg gccgcaggat cacggtcgcg ttgccgttcg gcatcgggaa cacgaccttc
    15481 acgcacggcc cgctctcacc gggaacggct tgcgtcatgt agaagccggt gtagagcacg
    15541 agctcttgct tcgcgagtgt gcggaaccag cccgtgtact tcacgcggcc ttcgggctcg
    15601 cgcatcaaga tgatctcact cgtcatgccc gtcgcggtgt cgagcggcga gagcgggaag
    15661 ttcagctggt tgacctggcg gctgatcgtc gtgacgagca ggaacagtcc gatgtttgcg
    15721 ggaaagtacg ttcggctcca cacgtcgaac gtgaagtcgg tcgtgttctc gtagaagttg
    15781 cgcacgagcg ggtggacctt cgcaggatcg aaatcaccgc cgcgcagcat ctcgaagctc
    15841 ggcacgaggc cgccgctgtg agctttgcgc tcgaccgaga ggccttcgcg ctccgcgacc
    15901 gattgaaacg gcgcatcacc gatcacgcga tcgccgagcg ggccatcgag ccatggaatg
    15961 tcggcgcgcc gatgcgtgcg accgaagatc cagatgagcc cgtagatgaa ccacgcgccg
    16021 agcgtcgcga tgatcgctct catggtttgg gcaccaggcg atagccgttg cagctcgcgt
    16081 cgaccgcgta ctcgacgccc ggcgctgggc cgaatggcat cggcgttttg aagtccgggt
    16141 gaaagcactc gagaacttcg atgggcttgg ccttctgatc gttgcacttc gcgctgagcg
    16201 gccgcttcgc gcgtttgcaa tcgagcgtca tcttcgcggt gcccgtcgag acatcgagct
    16261 tcagcgtctc gccatcggtc gcggtgccgg tgtagcgctg cgcgccttgc gcggtctcga
    16321 cgacgagtag cgcgctgccc tcggcgtgac gcagcgtcca cgtctcggcg cggtccttcg
    16381 tgctgtagcg aaacacccag gctggcgcgt cgagcacggc ttgtggcggg ccggcgtcgg
    16441 gctccgctgc ggcgtcttgc cgcggctcgg gcgtcgcagg cggcgtctcc gtcggatgct
    16501 cggcgggcgg tgccggcgag ctgcacgcag cgatcaggag aacggccaag cggcgcatcg
    16561 gccgcagcat ctcacaggtc aggacacgac gagccacacg gcgtgagcac gatcgttccg
    16621 atatcgacgc gcatgcccgc ttggatcgtg acgtcgggaa ctggcgcgag cgatgtcgca
    16681 agcaccgcgc cactgtcgaa gttgtatgcg attgccgaga tcgagtacgt gccacttggt
    16741 gcagagccgg tgagcgcgcc gagctcgcag cgttcgtctg tcgcgaagaa tggctcgtcg
    16801 ttcatcgaga acatgaccga gacgccgtcc gcgcccaccg acgcgcaggt cgcgcccatg
    16861 tcgaaggtcc acgcgacgcg gaacgtgtcg aagctgccga agtagagata cgcggtttga
    16921 tcgaagccat tgcgcagatc gacctcgtat tcggctgcgc tataaacctc accgctcgtc
    16981 atgaggagct ggccgacgac cttgaagcgt tggcgcgaga cgtccttgat cacggtcttc
    17041 gcgggaccac acgcgaccgt gacgttgtgg tgcgccgtcg gatcgtcgat tccggtgagc
    17101 tcgatcgaga catcgccgat gccgggctgg tcgcaatcgc cctcgtacca ctcgacttcg
    17161 acatcgggca gctcgggatc cggatagatc cgcatgcacc cagtgagcgc gacgacgacg
    17221 agcaggctag cgcggagcaa actcgactcc gatgtaagga atgatcggca agccacgatc
    17281 gtcttcgata cgatatacgc cgttctcgtc gggatacgag cttcgaaatt cgacgttgcg
    17341 gtgattcgtc acgttctgca gatcgaggaa gccgatgacc gagccccacg actgcttcca
    17401 cgcgtggtcg acgcggacat cgagttgcca gaaggtcggc agccgttgga gcacgggggc
    17461 cggttcctgg tcgaggccag tcgtcggcgt gccggcgggg acccagcgaa gaggattgcc
    17521 agacgcgacg gtcaggcgcg cgccgaagtt ccacttgcgc gccgacgtcg cagcgaggag
    17581 gttcacgcgg agcggttgat caaggccgta cgggacccag ccgagcttca gtgccgggtc
    17641 gtactgacgc ttcgcgcgcg accacgtgaa gttggcgagg aagcgatagc gaggcttgtc
    17701 gtagcgcatc gatgtttcga tgccgtacgt gcgttgccgg cccacgttgc cttgaaatcc
    17761 gtacaggccg atttgctcct cgaggagctc tttgaagatg agctcgagat cgatcgcaga
    17821 ggaccgcacg ttcttgtcga cgatatcgac gagggtatgc cgtccctcgt gatagaagcc
    17881 ggtgatcgaa gcgaggacgc cttcctcggg cgacagctcg acggcaagcg tcgcctgatc
    17941 gacgtacgag ctctttgcct tcaagttgtc ggcgaactcg tcgaagtgag cgggcgaggg
    18001 cggctggtga aagcggccga gcgaagcgcg cagcgtcgac gtcgaggtga gcgtcgcgtg
    18061 tgcattgacg cgagggtcga ccgcccactg ctcgccgagg ccgaaccgct cgaagcgaag
    18121 accggggcgc accgagaggc gatcgccgta ccagtgccgc ctcgcttccg cgaagatcgc
    18181 agcgttcgcc cacagcacgg tgttgcgagg gaacggcgtg tcattgtcga tgtcctcggc
    18241 cttcgtctgc ttgatgcggc cgaggtagcc gccttcgaga tcgatgccgg cgcgcaggaa
    18301 gccgtggtca tcatcgcgca gccactcgcc acgtccgccg aacagatacc agcgacggct
    18361 gatgtcggtc acatcgggca ccgtcgtcgt gtcgttcgga tagtccttct cgtagagcgt
    18421 caggatattc gtgccgagcg tcggtgcgag cgtgacgagc gtcttgtcga tctttctctt
    18481 gtacgtgaca ccggtgcgca cgaagccgag gttcgccgcg atgtggccat ccgcgtcggg
    18541 atccatcggc ttcgcggcgt cggagctgtt cgcgatccga tcgagcgagc cgaacaggta
    18601 cgccgcgagc tcgcccttct tcgcgggctc gccccacact gcgcgcagca cgccatcgct
    18661 gtagctcggc agcggatcgt ttcgcgtcac gagcggccgc acgaagaaat cgaagtagga
    18721 gcgccgcagc gtcaagagag cagcaccgtt gcccgggacg ggaccctcgg cgctgacgag
    18781 cgagtgcagc aggctcacct cgccgccgac gcgatacgcg tcaccacgcg gccgccgcgt
    18841 cgtgagctcg acgacgccac cttgggttct gcccatcgat acgtcgaagc cgctcggcac
    18901 cacacgcatg ttgtcgagcg cttgcgtcgg gaacacgccg gtaatgccgc cgaagtgaaa
    18961 cgcgagcggg atctcgacgc cgtcgatgaa cacgctcgag tcacgcggcg acatgccgcg
    19021 cagcacgacg ccgccaaacg agaacggaat gcgggacgca gcgggaagga tcgtgatcgc
    19081 gcgcagtgca tcgttcatcg cgccgggcgt cgatgcgatg tccttcgccg tcatcacata
    19141 ggcgatgggc ttggcctcct cgggcgcctt accggagatc tcgatgacct cgccggtctt
    19201 cttcgagagt cggatctgca gccccggctt ggccgcgacg gtgacggctg catatccagc
    19261 cgctgtgacg gtcagcgtgc cttcaccgtc gacggtgaaa tcgccggaat catcggtcac
    19321 cgccgcctgg tcaccgagca agaccgacgc cccggcgata ggcgatccgg tttcgtcgtc
    19381 gacgacgcgc ccgcgaacct cggtatctgc cgccgcaacg cgagtaagcg ccgcgacgca
    19441 cgcgattgca ggcacccagc gcatgtcggt cgcacgatac ctgactcgcg cgctgccgaa
    19501 gtggatgcct gcgatctctc agctcttggc gacgacgaat gcgcgctcgg ttgccgagag
    19561 ttgccgctgc gcgtgatgcg caaccgcggc gcgtgcgtcc gcggagtcga tcgccttgag
    19621 agcctggatg agcgagatgc gcatttgctc gtcgggctcg tggtcgagtg ctgtggcgag
    19681 aggcgctacg gcagtcggcg tcccgcggct gcgcagcgcg agagcagctt cttggcgcac
    19741 gcgcatcggc tcgcggtcgt tggtcgccag ctcgagcagc agtgcaccgt ctccatcaca
    19801 cgccgcgagc gcgtggatcg cgttcgcgcg cagcagcaca ggcgcctgga ggtcgcgtgc
    19861 gatcgcggtg agcgccggag cggccgctgt gtcgtcgatc cgtgcaatcg ccgctgcggc
    19921 atgcgcagcg ctcggaccct ccgcgcgcac cgtcgctgcg aggtactcga cgttccctgc
    19981 ctcgctcgcg tgctcgatcg gtgtcagcgt gcgcgcatgt acaggcgcct gttcgcgatg
    20041 cgcgggctga cgcgcgagca cgagtacggc gccgagcgcg gcggcgagga ctgcggcgac
    20101 gatgccgcgc ttcatggctt caccttgatc gagtcgctgc cgatcaggtg atcgccagcg
    20161 tagagcgtca cggtgaccgg ctggttcggg ttgatcagtc ctgcggcaat gcctgccttg
    20221 acgctcgccg agaacgcagc gcggttgaac cgcaggtcga cgtgcgtcgc cgacggacca
    20281 tcgcactcgt caacctcggt gtcgaagctc gtcttgtcgc cggccacgtg gacgcgaccg
    20341 agcgacgccg cgaccgcagc accgccaggc acgcgcagcg tcaagttgcg cgcgttgatg
    20401 ggcaccaaga tagcggcgtt cacgccgcgg acggacatcg tcacccagtt gccctttgca
    20461 ggcaggcgga gcgactgcgg gtggaacatc tccgtccagt tcgcgacgcg aacagtcgct
    20521 gattcggtgc cgacgttccc tgcagcatcc gttgcagagc agctcaccgt gagcgcgccg
    20581 tcgagctgac ttgtcaggtc caccgtctgc tggaactgcg gatcggtcgt cccgcccagc
    20641 gccgtgctgc cgaccgagca gctcgccgac gcgaagtctg catcgcggac gttgatcgcc
    20701 acgagcaggg acgtcttcac gactgcaccc ggcgacgggc tcgtgaggaa gctcgtgatg
    20761 gcgtcgttgt ccgcgatcgc atgcacctgg gcttgcgcgc tcacgttgcc ggcgcgatcg
    20821 gtcgcgactg cgctgacgct caacggccca cttgcaacgg acgtcgtgtc gaacggaacc
    20881 gaacagctcc ccgatgcgca cgatctcacg accgcaccgt tcacgaggat gtcgagctgt
    20941 gcgacgccgc tcgttgcatc ttgcgcttgc gcgacgagcg tgacggtccc gcgcaaatac
    21001 gcgccttcgt tggggccgag caagctgacc gccggggccg cgttatcgac gacaaacgtc
    21061 gcattcgccg ttgcctggtt gccgacgcga tcggtcgacg ttgcgcgcac ggtgtgagtg
    21121 ccatcctgca aggtccgcgt gtcgaccgcg acggtccacg ccgcgccgga tgccgccgct
    21181 gcgagcgtcg cgccaccgtc gagctggaat gcaaccgtct ccacgcccgt gatcgcgtcg
    21241 acgctgtctg cggaaagctc gagccagccg cgatagacgg agccctcagc gggtgacacg
    21301 agcgcgagag tcggcgcgct gcggtcatag cgctgcatgc tcgagaacat cgcgacgtgg
    21361 tggccttcgt tctcgacggt tgcgcggatc gcgttgtcgc cctcgtgcag caggtacgca
    21421 accgcgatca cgccgccgcc acgcggcaaa tggtaagagc tgccgtccga caacgacacc
    21481 gtcgtcgcgc caacatcgtt gacgaacagg ttgaacgtga caatgtcgcc aggcagcggt
    21541 ccgcgcgata cgccatcgac gaggtcgctc acgagctgcg gagcctgccg atcgacgagc
    21601 accgtcgcgg atgcatccgc agtgttgtcg gctgcgtcgg ttgcgacgat cgcgatcgag
    21661 ttcaagccgt tcgccggcac cgacgcgacg accctcaccg ttccgtccgc gggaacgacc
    21721 gcgggttgac catcgatcgt gacggtcgtg ggcgaagcgt cggtaatgcg cacggtaact
    21781 gcgacgaggc cgtcgtcgcc gatcagctgg cccgacagcg gagacaacac gacgatctcc
    21841 ggagccacac tgtcacgcgt ggtctgcgtc gacagtgcgt tcgtattgcc ggcagcgtcc
    21901 gcggccacga tctcgatgct gttcgaaccc tcgacgagcg acagctctgc ttcgaacgtg
    21961 ccgtccgccg cgacggccac cgttgcatga ttcacctgga cgctcgccac gcggccgttg
    22021 tcggtcacgc gaccgacgag cgtcgtcgtt gcatcgctcg tgtagtccgc gcgtgaaacg
    22081 atgctgatgg tgggcgccag cgtgtcagcg ccacacaggc cgctattgca gacgagaccg
    22141 cctgcgcagt cgttgttcgt cgtgcacgcc ggaatgccgt acgtcatgta cgacgtaccg
    22201 cgcccgtaga tcgtgccgtc gagcgatgct gcgtagtcga gcgcgcccga ctggctaacg
    22261 tcgaagcgga aggacagtgc accggcttcg agcgtgtact tgccggggat caagtaggtc
    22321 tggccgatca ccgatttcgg ggaccacccc tggatcagcg cgacgtcgta ggcggtcgta
    22381 ttgggcgcga ggtgcatatc aaagcgaatg aggtagccgg cgacgttgag cgtcgacgta
    22441 ccgcggccgt gtagccagcc atcgaacgtc gccgcatagt cgacggcgcc gctggtaccg
    22501 acgctgaagt cgacctcgtt cgccaggtac tgcccgccga cgaacgtcgt cacgtagtac
    22561 gagtacgcgc ctggcaggac gtagacgtct tcgtcagtgc gcgtctggcc ggcgatcaag
    22621 ttgatgccgt ggttgtaacc aacgagcgga gtcgtgatgt ggagcttgac gccgtcgacg
    22681 tacagcgtgc tcgtgtttgc gccgcgcaga tacgtcagcg aagggtcata ggtgacggtt
    22741 cccgtcggcg agacatagaa gatgaagaag ttcgcgccat actgcttcgc gagcgtgtag
    22801 ttcgtgacgt agtacttgta gacgccgggc agcagcgaga cggtcagcgt cgacgatggc
    22861 tgaccgctga tctgggcgac gccgtggttg tagccaacga gcggtgtctc gaatcggatc
    22921 gagaagccct cgacgatcag cgtgctcgtg ccggcgccgc tgacatacgt ctcgacggtc
    22981 ggcgagtacg tgaccttgcc gtctgacgtg accgagaagt cgaatgcgtt cgccgtgtac
    23041 ggatagtcat tgccgttctc cgtgaacacg aagtactcgg tcggtccggg cagcacgtgc
    23101 agcgaatagc tcggcacgcc tggatcggtg agtaggttaa cgccatgcga atacccgatg
    23161 atcgatgact gcacgttcag cgtgtagccg cgcacgtcga ggaggttctg gcccgttccc
    23221 acgtaggccg atgcaccggt cgcgacgctc gtctgaccat ccgcgccgac gttgaacgtg
    23281 aacgcacact cgttgtaccc agcggacgag tgcgtttggt accccatccg atagatgcct
    23341 gggagaacgc gaattgcgta ggacgtctga ttcgggaggt tgacgtacgt gatcgtttgg
    23401 tgctgcgacg agagctggct cgtcacgttg acgaggacac cgttcgcttt gagcgtggtg
    23461 gtcccactac ccgcgaacac gcctgcgagg gacgcgtcgt agttgacggt tccgtcgatc
    23521 gcgaccgaga acgcgacgcg gttgacggtt tgcccggccg ttccccatga cagctcgccc
    23581 agcgtgtagt taccggccgg cagatccgcg gtgacggtgc cgctaaacga gagcgagcgt
    23641 ggcccgctta tgaagatcgg ctgattcgtc gacagcggac gagcatcgat cgtgacgttc
    23701 gcggcagatg cgtcgacggc aatcccgcca agacacgcaa caaatgtcac tgcaaacagc
    23761 gttgaacgca cggatgtcat caagcggtcc ccccgacggc tctggtcacg tcgtctcgaa
    23821 gagagacgaa tgtccatagc aacagctcac gcgggttcgc gtcaaggcaa cgtgcccaag
    23881 ctcgcaggcg gttaacccag acgtaccccc tgatccgatc acgcaacttg cgcgtacgca
    23941 tcacgaacac gtggtcacgc gcttgcactc agcgaagacg cgacggatgc catgagcgac
    24001 gacttgcttc tccacggctc catcctgctc gtcttcgcat ggctcgcgat cggcagcctg
    24061 ggggcgcccc taccggaaga cgcagctctc ctcgccgctg gtgtgctcgt gcagcgcggt
    24121 gcggtccatc cgctcgtcgc ggtcgtcgtc gtgttgatcg gcgtcttggg cggggattgc
    24181 gtcctgttct tcttgggacg ccgccttgga cctgctgcct acaagcgaaa gctcatccag
    24241 aaggcgctcc cgccgcaccg gcgcgagaag atcgaacgtg cgtacgagaa gtacggcggt
    24301 cgcctagttt tcctcgcgcg acacgtcgcg ggattgcgcg cggccgtgtt cgcgatggcc
    24361 ggcattcacg gcatgaagcc gcgccggttc ctcgcgtggg atgcggcggc cgcctgcatc
    24421 agcattccgt tcgtgatggg cctcggctac gtcggtgcga agaacgtcga ccggatgcgc
    24481 gctgggatcg ccgaagcccg gcactacctc ctcgtcatcg tcggagtggc agtgctcggg
    24541 ttcatcacgt ggcgccacct tcggctgcgc agcgcggcga agctcgaagg ctagcgaacg
    24601 ctgggaaggc gagccgcggt gcgggtctcc gccggcaggt tcttgtatgg atgcgacagc
    24661 atcagctctt cgaacatgac gaggagctcg ttgccgagcg ccgcgcgttc atccgccgag
    24721 agcatgtctt tcacctgcgg gaagagcttc gcttcctctt ccttgtgcgc gtggtgaccg
    24781 acctgctcct tgaggacctt gagcttggcg tcgaacgcgt cgtcgtcggg gctcagcgcg
    24841 atgagatcgg cgaggaggcg cttcatcgag aggtgctcct cgaccgcttc acgcagcttg
    24901 tcctcggtac ccttcgccat gatggccggg tagaagatct tttcctcgac cgtcgcgtgg
    24961 gcggcgagct tgtcggcgag ctgcgtgaac agcgcgcgaa cgttgccgtc acgcttctcg
    25021 atgcgcgcga acaagtcatc gacttccgtg tgctgcgacg tcagcagctc gaggacgtcc
    25081 gtcgtggtgt ctttgccgaa cagttttcta agcaatccca tgattccagg ttctgcgagc
    25141 ggaatgccaa gtcgtggcgc gctacagttg tggcgtgtgg gcacgcgttc acaaggtgga
    25201 taagatcaag gccttgcccg gcggcggtgc ggtcgtcgtg ctcgaggacg agcgaaacgc
    25261 gtctcacatg cagcgcgtgc cgagcctctc gtcactgatc gcgatcgccc gcatcctcgc
    25321 cgcgaagcgc ctgctcgcga tcaagttcga gggcaaaggc gagattcgct acgcgacgaa
    25381 cgcgctcgtg ccgagcttcc tctccgagac gatcgctcgc gcaggtgccg cactcaccga
    25441 gcgcgacggc gaaaagatcc tcatgcctgc cgcgccttcg ggtgtctctg cactgatcga
    25501 cgtcgggttc tcggagctcg cacaccacgc gcgcggcacc gtcggcatcg tcgatatcgg
    25561 agccgcactc aagcacatcg agcagaagcg gcgcgcctct cccgtcgatc gcgacgcgaa
    25621 gcccgaggtc tactggcccg ctgtcttcga gcttgccgcg ctcgccggtg agcagtcgcg
    25681 tgcgcgcggt ggtcggtggg tcgagacgac ggagctgccg gtcccgttcg cgctgaagtt
    25741 ccctgaaggc cagatcgcaa agccaacgga gatggcgcag aagatcgtcg agggtaagga
    25801 cgagctcgac gccagcatgg ccgacgtcta agcgcgcgcg cgttgcagcg ctgcgatggc
    25861 gagcagccgc gtggagtcga gcgtcggcag cgtcgagttt gcgggaccga ggacgagtgg
    25921 cagctcggta cagccgagca cgacggcatc gcagcccgca tcgcgcagcc gatcgatgac
    25981 ggcctcgagc gcgcgcacag actcggtgcg aacgatgctg gggacgagct cgtccatgat
    26041 gatgcggttc atgaggtccg cgtcgccgga agacggggtg accgcggtca tgtcgcgtga
    26101 cgcgagcgca ttgggataga tgtccgaggc gacgagccag cgcgtgccca tgatgccgat
    26161 gcgcttgtaa cttcgcgcgc gggcagcatc ggcaacgacc tcggcgatgt gcagccacgg
    26221 cgtcggcgat cgctcgacga tgaggggcag cgcttgatgg atcgtgttgt ccggacagat
    26281 caagaagtcg gcgcccgcag cggcgagctt gtccgcgctg gcaagcatga gctcgccgac
    26341 gcccgcccaa tcgccgcgct cgagccggtc gacatacagc gcgagagacg gcgtgtgcat
    26401 cgtgatctct ggatgctcgt gcgagccggt gagccgcgcc gcctcgctgc agatcgttcg
    26461 atagcagagt gccgcgcctt cggcggagca gccgacgatg ccgatgtgct tcatgcgccg
    26521 atcacgacgc gccggccctc cgacggtgcc agcgtgatcc cacgccgcac gacgctcgcc
    26581 tcgccgccga ccgcgcgcat gcgcgtgcgc tggacgatcg tctggaccac gatcttcatc
    26641 tcgagctgcg cgaatgccat gccgatgcag cgtcgaatgc cgccgccaaa aggcagccac
    26701 gtgtacggat cgggcttcac gccgagccac cgatcgggat tgaaccgctt cggctcggga
    26761 tgcgaggcag cgcgcatgcc ggagagatag atcgatggcg cgatgcgcgt cccgacaggc
    26821 agatgccacg gccccatctg gaatggcttc gcgacgacgc gcccgacgag cgggatgatc
    26881 gggcgaatgc gcagcgtctc gcggatcacc gcgtcgagat actcgtcctt gccggcggcc
    26941 acgtcctcgc gcaggcgcgt catgacggcg ggatgcgtga gcagctggtc gaacacccac
    27001 gcgagcgacg tcgccgtggt ctcgtgcccc gcgagcagga gcgtgacgag ctcgtcgcgg
    27061 atctcgacgt cgctcatcgg ctcgcctttg tcgtccttcg cgtcgagcat catcgacagc
    27121 acatcggtgc cacgcggtgc ggcgcgccgc tcggcgatga tcctatagag gaggtcatcg
    27181 acggacttct tcagcttcgc gacgcggagc cacgggacgg tgaacggatt gaagccgagc
    27241 agcccgggaa acatgagcca tgggttgctc aggatctcga gcaggtcgat cagctgcgcg
    27301 cgcaggccac cgtgcttcga agcctggtcg atgccgaaca ccgtccgcaa gatcacgtcg
    27361 agcgtgatcg cttgcatctc cgaatgcagt gcgaagtctt tgccgacggg ccagcgatcg
    27421 acggcctcgc tcgtgatcgc acgcatcgtc gaggaatacg cctgcatgcg ctcgccgtga
    27481 aacgacggca gtagcaaccg tcgctggcgc agatgctcgg cgcggtcgag cgtcagcagg
    27541 gaattcttgc cgaggagcgg ctcgaggagg accgcgttgc cgacgccggc gagcaacgtc
    27601 tccggatccg cggagaacac ctgcttgatc atgtccggat ccgcaacgat gacaaaatca
    27661 ccttcgcgaa agccacccca cgtgaagacg tcaccgaact cgcgctggca ttcctcgagc
    27721 aactggatcg gcctcaggac ccagcgcgcg agctgcacga gcggaggtga ctgcgggccg
    27781 ggcggcaggt cacggcgcgg ttgaatcttg cggaggccgc gcaacaccac gcccgcggac
    27841 atcgcggcga cgccgttcga acgagcagtg gtggccatgc cttgacggta ggggagtgtg
    27901 gcgtccttca caaccacgat ccgatgcggc tcggacgttg atcgttgcct agtccatctc
    27961 gatcatgtcc gcgaagctgg cgtcggcttc gggcccaggc tcgtcgatct gctcgggatg
    28021 cgcggcgagg cgcgacggcc atggcgcgag cgcgatacgc ccgggctggg ccgccaccgc
    28081 ttcgagctcg cgacgcgcag cgctccattt cttgcgccac gactcgtcga gcgtgcggtc
    28141 ctttgcgcgc ttgtccgctg gctcggtcgt cgactgggcg aggtcgcgcg cgagatcgag
    28201 cagcggacgg cgaagagcct cgcgcagcgc gagcgttcgc tttgcggcat cgacagccat
    28261 gcgcgcggtc atgtcggcgt cgggcgcgcg accacgagcc gcgatgtcgc ggacgcgatc
    28321 ttcgagcagc gagaggttgc ttgcgtacgc gacgaccgtt gcggcgaaga ccttgcgcgt
    28381 ctgcgtcgga atcgccgtgt cgtcggcaag cgtgtgatcg agctcgatcc gcatgacacg
    28441 catcgcaagc tcgtcgatgt cggcgattgc ttgggcgagc gtcgcgtcgc gcccttcgag
    28501 atccgcgagc gtgatggctg tacccgcgtg ctcgcccgcg agtacttcct cgcgaacgag
    28561 gcgcgcgtac tcggccggct ggagctcgag cgcggtgatc gaggcgcgcg agtggactcg
    28621 atcgagtatg cgcttggcgc tcacgtcgtg atcctactcg gcgtcgggcg gtgcggcgaa
    28681 cagcgtgcga attccatgca gcgctgccgt cggaaagatc gtgctgtgat gctcggttgg
    28741 catcggctcg taggtccagg tcagaccggg gggcgcaact tggcgcatcg ccgtgagaag
    28801 attggcgatc acctgttgag tcggctccca gtcggccgtc gcgacgtaga gatggcgcgg
    28861 gcggccgtcg gccgggaagc gcgcgaggta catcgtcgcg ttgtcgacga gtgattgctc
    28921 gttccagaac acgctcgggt ccgccgagat gtagccgtcg aataacgtcg gatcgacgag
    28981 caacgtctcg accacgaata ggcccgcgaa cgattcgccg acgagcgccg actcgccgct
    29041 caccgcgtag tgtgccgcga tgaatggctt cagctcgtcg cggatgaact ggcggaagcg
    29101 gtcggcgcca cccgcatgcg gcgcgatctt cttctcttcc tcgagcgtgg tcgggccgac
    29161 gagatcgcgg cggcgctcgg tgttctggat cccgacgacg atcaccgggc ggataatcgc
    29221 gttcttgatc gagacgtcga ccgatcctac gacatgcggg aagtcttcgt tcatgccgcc
    29281 gtcgggcatg tacaacacgg ggtagcgcgc gctcatcgaa tagtccggcg ggacgtacac
    29341 gttgatcgtg cgcttctcgc cgaggatctt cgagtcgagc gtgtacgtca tgccgagcgt
    29401 cgcctgcggc ttggcaggtg tggccccgac gtgatcgacg acgcgcgaag acgtgcaacc
    29461 gaccgcgaac aacattgcga gcagcgcaat tctcacgggc gcgagcatat aggatacggc
    29521 gcgtgcaggt agagccaccg cgcgtgggac cgaagccgcc gttctatacg acgctgtact
    29581 tccgcgtcct ggtcgccatc gcggcaggca tcgtgctcgg cgtcgcctat ccgacgtttg
    29641 ccgcgaagct gcagccgctc ggcaccggct tcatcaagct gatcaagatg acgatcgcgc
    29701 cactgatctt ctgcacgatc gtggtcggca tcgcgagcat gaagagcatg aagtccgtcg
    29761 gcaaagtcgg cgggctggcg ctgctctatt tcgaagcggt cacgacgctc gcgctcgtca
    29821 ttggcctcgt catcgtcaac gtgatcaagc ccggcagcgg catgaacatc gatccgcacg
    29881 gcgatccgag cgcggtagcg aagtacgcgg cacccggcca gatgaagggg acggtgcaat
    29941 tcttcctcga catcattccg gacacgttcg tcggcgcgtt cgtccatggc gagctgctcc
    30001 aggtgctgct catcgcgctg ctcttcgggt tcgcgctgca cggcgtcggg gagcgcggca
    30061 agccagtgct cgatctggtc gaccgcctcg ggcaggtcat cttcaagatc gtcggcctca
    30121 tcatgcatct cgctccgatc ggcgcgttcg gcgcgatggc gtacaccgtc ggcacgctcg
    30181 gcctgcactc actgctgaag ctcggcgagc tgatggcctg cttctacggc acgtgcctgc
    30241 tgttcatctt cggcgtgctc ggcaccatcg cgcgcattca cggcttctcg atcatcaagt
    30301 tcctgcgcta catcggcgac gagctcttca tcgtgctcgg cacgtcgtcc tcggagtcgg
    30361 tgctgccgcg catgatcggc aagctcgagc ggctcggcgc agggcagggc accgtgggcc
    30421 tggtcgtgcc tgcgggttac tcgttcaacc tcgatggcac ttcgatctat ctgacgatgg
    30481 gcgcgatgtt cgtcgcgcag gcgaccaata cgcatctgag cctggtccag caactggagc
    30541 tcctcgggat tctgctgctg acgtccaagg gcgctgcggg tgtcacggga agcgggctcg
    30601 tcgtgctcgc cgcgacactt ggcgttgtcg gtcacgtgcc cattgtcggc attgcggcga
    30661 tcgtgggcat cgatcgtttc atgtcagagg cgcgcgcctt gacgaacgta atcggcaacg
    30721 gtgtcgcaac ggtcgtcgtc gcgaagtggg taggggacct cgattctcag aagctctcgg
    30781 cggagctcgc gtcgggtcca ccacctacag aggcgtgagc gacgagaagc tctcacgcga
    30841 cgcgatggcg ctgcctatgc agcccgcgtc acagcatgaa gccattcagc cgagagacaa
    30901 tcatcgagcg ccgtcagaca tcgatgcggt ggagcgccat tttcggagga acgctcctag
    30961 ccatcgggct gtggacgatg ttgcaggtgc tgggcatggg catcggccta gccgccgtcg
    31021 acaccgacga cacaggaaac ctgaaaggca tcggcatcgg cacaggcgtg tggtcgttga
    31081 tcgcaccgct gatcgcgatg ttcttgggcg ccatgctggt tggcaggctg tgtggctcgc
    31141 gcgatcgcaa gatcggcgca atgcacggga gcgtcatgtg ggcgctcgcg ttgatcatcg
    31201 gaatgtgggc aatggtctcg gtcgtgtctt cgatcgcgca cggcgtcgct cgcgtgggcg
    31261 gcgcggccat tggcgcgacg ggcagcgtcg tgtcgagtgg cgtgggcgcg gcgagtcgca
    31321 acggcgacgg tgccatgtcg gcgctcggca tcgacacgaa cgatctgctc ggtccgatca
    31381 accagcgctt gaaggagaac ggcaagccgc cgattaccgc caatcagctc gaagcgacga
    31441 cgcgcgcggt tgctcagcgc ggcctccatc aaggcaagct cgatcgcaac gtcctcgtcg
    31501 atgaaattgc gaagaacacg tcgctgtcgc gcgccgacgc cgaagacctc gcgaatcagt
    31561 tcgaggacaa gtaccaggcc atggcggaca aagcaggcaa tgcggtcgac caagtcagcg
    31621 agaaggccaa agacgtggcg ctcgaaaccg ccgacaagac gggcaaggtt ctgttgttcg
    31681 gcggcctcat gatgctgctc tcgctcggcg ctgcgatcgg cggcggcatg ctcggcgtga
    31741 aaatgcgcga gcgtgaggag gcgtccgacc gcaccgttgt cccgcccatg tccggcggcc
    31801 tctgaacgtg ctcgaatagc cgcatggatc ccgaattctg gcgaggtaag tgggccgagc
    31861 gacagatcgc gtttcacgaa ggcaagccga acaacttcct ctcgaagcac gtgacccggc
    31921 tcaccggacg cgtgcttgtt ccgctctgcg gcaaggcgga agacctcgca tatctcgcga
    31981 gcgtaggcca tgaagtcgtc ggcatcgagc tcgtcgagag cgcggtcgcc gagttcttct
    32041 ccgagcacga catcctgccg accgtcacgc gcgtggaggg cttcgccgtc tacgtcgcag
    32101 gcggcatcac gctcatcgcc ggcgacttgt tcgctaccac gcgtgagctc atcggccgcg
    32161 tcgattcgat ctacgaccgc ggtgcgatgg tcgccctacc gcccgacatg cggcggcgct
    32221 acgtccatca gctgcgccag atcgcacctg ctgcgcaacg cgtgttgctc gtcaccgtcg
    32281 actatcccgc agacgcgatg aatggcccgc cgttctcggt gcccgaggcc gaagtgcgct
    32341 ctcaatatgc aaacgccccg atcgatgtca tcgaccaggg cgcggatccg cgaggcaggc
    32401 tcgacggaca gatgatggag cgttgctact ccatcgactt gcttacatcg gcgaaaccga
    32461 cttcttgatg aggtccttca cctttgcgac atcgacgtac gggttgctcg gcgagaacga
    32521 accggtcggc gaccagagga agtcgatgtg cgagttctcg ccgtccgagc agtagcggcc
    32581 accgatgacc ttgccgtagc cgttgagctc gaggatgtag tggtagtcgt catccgagac
    32641 gttgttcatg aagccgtgcg gcgtgttgct cgcgctcgac tcgctgacgt acttcacgtt
    32701 catgcgaact tcgtatagcg acttggcctt cgtgttgtag gtccacttgt cgcccgtcgc
    32761 gccgacgcac ttcatcgcgg tcttcgcgtc gaccttgtcc tgcttcgtga tttcgtagcc
    32821 gacgaccggc tggttccaca cttcgtagtt cgccgtgcgg tcttcgacga gcggcagctg
    32881 gcggaggccg aggaagttcg tcatcacgac gtgcagcgcg ccggggttca cgtcggagca
    32941 ctcgtcgttt gcggagccgg caacgtcgtg cgtgatctct ttcgcgttgc agcgaccgcc
    33001 gagcatgacg gcgtcggttt ggtcgaacac gttctgaatg agcgccttga tgtcgccgac
    33061 gtcgaacttg acgccgttga ccgtcaccgc gtgctgcggc tcgggaatca tctgcgaggc
    33121 aggcgtccac gcgtggcagg tgccccacca gccttgaacg ccgtcgatgc catcggagcc
    33181 gtacgtatcg atgttgccgt cgtggtcgtc atcgatgccg ttgtgcatga ggccgatgct
    33241 ctcgtactcc tcggtctgcc acttcgcggc cggaccggcg cactggttgt acgtcgccca
    33301 cttcgccttg ctgtcgcgac ccgagacttc cggttgaacg tcgcagccgg gcttgccgtt
    33361 gaatgcagcg tcgtacttct cgagcggcga cttcacggtc gcgccctgcc aacgtgcgtt
    33421 gtgcgagcct tccgaggctg gccagtaggt gtcggcccaa acggtcgctg ccttgccgac
    33481 agcttccggg tacgtgtctt tccacaccgg cttctcgcgc gtgcccttct tgggaagctt
    33541 ctcgagcttg tactcgaggt ggttcgccat gagcttgggg tcgttcgcgt agtcaaagcc
    33601 gtccgccttg ccatccgaga ccggcgtcgg gttgcagtcg ttgtcgccgg ggaggcattc
    33661 ctcgtcatcg gctccgtcca cacaaccgac caacgagacc gatccgacca gagccaggct
    33721 gatgccgagg attgcgttct tcatgatgcg agaggctctt cgcaccgttc gtgccacgcc
    33781 cacggtcgcc tgatttcggc ccgttaccgc atcgcggcga ctactcggct ttccgctgta
    33841 tcggcttcgc aggagcggac ttgccggcgg atccagcttc cacctgcccc ggcgcggtac
    33901 catcacacga ccagatgaag ccacgttaca cggtcctcga aaggctcggc ggcggcggcc
    33961 aggccgaggt cttcaagggc atctccgagt cgatgcaggg gttcaagaag tcggtcgcga
    34021 tcaagcgcgt cctgccgaac ctcaccagca acgagcagtt tgtcgcgatg ttcctggacg
    34081 aagcacggct ctcactgttc ctgcagcacg ccaacatcgt gcaggtcttc gacatctcga
    34141 aagcggacga cggcacgtac ttcctcgtca tggagttcgt cgacggctgc gatctcaaag
    34201 cgctcatctc gcacttcggc ggtggcggca aagatctcga tatcggcctg acgctctaca
    34261 tcatgatcga ggccgcgaag ggcctcaatt acgcccacca gctcgagaat ccgcaaaccg
    34321 gacagaagct caacatcgtt catcgcgacg tctc
//
LOCUS       DATLBZ010000189        15764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3906081, whole genome shotgun sequence.
ACCESSION   DATLBZ010000189 DATLBZ010000000
VERSION     DATLBZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15764
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3906081"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..1542)
                     /locus_tag="VMZ53_09955"
     CDS             complement(379..1542)
                     /locus_tag="VMZ53_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28826.1"
                     /translation="MRPLCVAAAFAGFAGLAVSAPAVADVGVGGQVGAGAQGASTYSS
                     VELRLDVAWTHARLGIGVRGVWDDDEFRASDWSSAGRAVTIVRAFEASGRVGETQLGV
                     AAGALAPARVGRIVDGYRVSLDDRWRTGVRAIARGGGDGGRGDELDASLEIDDVLEPA
                     LVGGGVRWQMAKPWGMHLATAIDPGDERRGAASVGVVEVGAQRLFEGEGARAELGGSV
                     TAEIMIGAGALAYGSGEIEKWGVTWSARGDVRAGSGAGGSLFGPLYRIERVAHDGRMG
                     IVDEAKMRGLGGVGAGIAIGARAEAGWLELEARERPAIGPLFVVGAGAPMGARLQAAA
                     WAAIARDDAAGAGEVRVAWAKRLFSALQAARIYRESDDAAVWTVTAWFGATTE"
     gene            complement(1645..1938)
                     /locus_tag="VMZ53_09960"
     CDS             complement(1645..1938)
                     /locus_tag="VMZ53_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUS28827.1"
                     /translation="MIRALRPVVPVIEAQDRDLADQIRRAASSVALNLGEGQRSEKGN
                     KRKHYAVAHGSANEVRAALLTAVAWGWLEDASEVLARLDRLLALLWRLTHPRA"
     gene            complement(2469..2837)
                     /locus_tag="VMZ53_09965"
     CDS             complement(2469..2837)
                     /locus_tag="VMZ53_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28828.1"
                     /translation="MRRLAVALVVLLAFVAVGGDVASAQLPPAKPYAQPGPPKPGAAN
                     SQEPWQPPPKPTEQQQWQEMKNQERPGRSGFWTSPYPAKHGAYRWRLLGVGVVILAIM
                     GALMVWLIRRPRSVTQRPRV"
     gene            complement(2849..5194)
                     /locus_tag="VMZ53_09970"
     CDS             complement(2849..5194)
                     /locus_tag="VMZ53_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HUS28829.1"
                     /translation="MSRRLMWAGGITLGALLLLLIGLGVIYPRVGAYMIRKRLSERLA
                     TRIDRKVTVGDIDVSLGHAVLRDVNVRGKLDGDMPLVHIERIDVDFATGASLVGTIEL
                     GDAVVTDMMVTLRRDQFGHDNVRDIVDRLRGNGEKKSGGSSGSSSQRPKKLTVKGIRL
                     LADDAITGTTALIDSGDATWTPDVTVAHLRGVSATTPNAPKAEIKGIEIRKAAGSPPV
                     VTVEDGAFSLWPKMSLSGIGGTIVADPAKHGEYVLDFAGGYGGVPGRLWTAKGGFNVD
                     EVTASLDLVAAKFQLDRLAPILAKSGVVDYQSTSVDAELHVALDHDVAQFSGDMNLSG
                     LNIGHPMIADKEVHDVDLSTNLAGSFNRATRTLELTQGDFLLFDVPFSVTGTVYRPRR
                     DVVVPEAAQTKKDPKDLRLDDDADEPLAVATRGPYGIQLAKLRLVVPPISCQRFIDAI
                     PAEMAPSMKGYKIKGMFDTDVKLEIDWTNLDATVLDGHVGIKHCKVVDDPADSPKRLI
                     EPFEHFVEVEEGEWISFIVGPENPDFVPITDISPYLIKSITTTEDGAFYQHHGFIVSE
                     FKSALIRDLKAGKFKYGASSITMQMVKNVLLFRQKTLARKLQELFLTWHVENTLEKDR
                     ILEIYFNVIEYGPALYGIGPAAKEYFGKSAKDLNPVEAAFFSTILPNPKDRYKQFCQG
                     QVFKWTQAKIDRILALELKRGRLTQEEYDKAVATPLVFAKNTHEVEHKAPNGMILVET
                     ETEKECVERVKTTIENARPTAPPLPGAPTSTKKKSKKKSKR"
     gene            complement(5246..5689)
                     /locus_tag="VMZ53_09975"
     CDS             complement(5246..5689)
                     /locus_tag="VMZ53_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28830.1"
                     /translation="MSPRIWLAAFVVSAALASPAFAGDADCNFIEITATNEKAGAIDG
                     DLKPLEKKLKKPPFTAWNTFHKLSSGTTALTKNKAEAIKLKQGGASLMLRDRTEKRVE
                     LTVAWDGADGKRLLDAKPAIKSGDWLLLVGTNAKDDGHILALTCK"
     gene            complement(5686..6252)
                     /locus_tag="VMZ53_09980"
     CDS             complement(5686..6252)
                     /locus_tag="VMZ53_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28831.1"
                     /translation="MKDADLMMLGDSELDEKQMRELEEELARDPVARGKLDALGEVGE
                     LVRGHLELSADAVPSKKFDALWREIDKGIDRASEKAPATSTKEAAPAPGFLRRVGRWF
                     DTYRGHIITGAVSAGAVAMLALVLRSPDKTITPTGKPIDTMPVVHQPADIQSLDTPGG
                     TGTVFNLEDEDGDTTVIWVTPEDSVEGI"
     gene            complement(6249..6926)
                     /locus_tag="VMZ53_09985"
     CDS             complement(6249..6926)
                     /locus_tag="VMZ53_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS28832.1"
                     /translation="MNKEGQRASTGRDPVDDRELVESARKGDRDAFKTLFERYHRRAY
                     ALAFGVLRHQDDALDVVQDAFIKAHKYLDKFEGNSSFYTWLYRIVMNLAIDHLRKHRR
                     VKPVELDEAHLDGTTTDDSLLPKFLGGNPGRALMDKQIRARIDQALGELSENHRAVLV
                     MRELEGMSYEEMAQQMGCSKGTIMSRLFHARKNMQKRLADLMEGRAVANDESHDEDDA
                     AASGSRS"
     gene            7088..10015
                     /locus_tag="VMZ53_09990"
     CDS             7088..10015
                     /locus_tag="VMZ53_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28833.1"
                     /translation="MYRHEAARAAFTALAALGALAVVLPLVGIAISKSRTTALTILSI
                     GGLAAMLVVIGAIVVGIVAPRRRWGRDRELARWVGTRQKSVASDLLSSVELAEAPPRP
                     GSPSTDLVDALIEQTASELSTIEPATLLPEREVPRARLLALFAIAVNVALLLLAPSFM
                     HRGWHHLVSAPRSPFDGAELSNVPLVGDLDATLTFPAYSKRANLELKSSSGDFRGLPG
                     TKVQISGHLLVPATSAELVIEQGNSEPKRIAAKIENEQLTAQLTIEQSARYRIAVETP
                     SGDRKIEATPRAIEAEPDQVPAVQLMAPADRLDVANLRRVELAYVIEDDFGVTTAELV
                     WEGGKDRGKKPITLGESEQGRVQGKLMWDIAELQVQSGGEVHYWIEAKDNDTIGGPNL
                     GKSRELVLRVTSPRERHEETLDRQQAVAEKLLKNLGTRLVGFGEDTTARETTSHDLRD
                     SIVELSSINGAYEADPHASDLMRKALGGIHDRLDRLATMESKLLPKTATKVPTGRFAA
                     IDPPLVRELEDDLITLADWLDRERMEGLLDVSDEIAAHQKRLADLLNQYNKTKDARLL
                     EEIDREMKALDRAFAELEKHRKGMAEDVLDQYVNRDALAAEDGTNCMAEVRALIAAGK
                     TAEAQTKLESCQQQHARGASALEGSLAQLRGDKFNDEQKKLDEVMNELADLAKDQDDI
                     AAEANRIFEAYARKADDMAREHRKEASKKVGALVEKLRKRLGAVNETGLTPFAKEELD
                     IVERRVADVEHMVADGDLAEALGMARQAKTSLDTIAGELEAALDDDPKSKWADATQDA
                     LDGIEHTAPVAKELIDELSALSPKPEQIMSGDDLKALDRLRRREAMNKQRTQKLGDRT
                     KQMGQDLPGDAAAELGKKLGAAADHMQKADDRMKGKDPSGARESTRAAADALAKARDR
                     ARSAARQAQEGSINDEPIRIPGADEYKAPERFRENLLEGAKQKPVDGFEDMTKRYYEE
                     LVK"
     gene            10012..11562
                     /locus_tag="VMZ53_09995"
     CDS             10012..11562
                     /locus_tag="VMZ53_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS28834.1"
                     /translation="MRRAIVFAVLLVASTAHARRDRTVTGDVIKAARFLTSSRIDEAK
                     TLLADLEKRAPDAVEVKWLQAELAFQTGDYAAAVKLLDKIPDTEVDGMVGQTRKLAQQ
                     SLGVTETFVEKKSPKGHFIVRYAQGSSDAAIADLAGEVLDSTWDTIGDDLGLKPADPI
                     RVEILGAPADLAKLSPLTEAEIEKTGTIALSKYNKLMVVSPRATLFGYPWMDTLAHEY
                     THLVVSRIANDAVPVWLQEGIARFEQTRWRKAPEVALTATEQALLTTGLKKGRLITFD
                     EMHPSMAKLPSQDAAALAYAEVYTLVGWIHDKIGYEGIRELLTAQKDGKSARRAVAEA
                     MGQTWTKLEADWRVHLKVSSDAKVKAGKPIKFKAGGQDSENVGLDGVNARARKFARLG
                     GMLRARNMLEAAAAEYEKALNAGNDPFVAGKLARTLVELGKNDRAIELATPLAAADEH
                     DAVAAVTLGIARSARHEWKEAIAAYEQALRVSPFDPQTRCGLAEAYSEIGDSRSARER
                     SACDELKN"
     gene            11563..12636
                     /locus_tag="VMZ53_10000"
     CDS             11563..12636
                     /locus_tag="VMZ53_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUS28835.1"
                     /translation="MTKPADITPIRQEAATEVNPTVPAPSDAPHKSDLEAVAELADAH
                     KQIVTEIEKRIIGQRRVVDQLLVALFARGHTLFVGVPGLAKTLLISTLAETLNLTFQR
                     IQFTPDLMPADITGTEVLEEDHTTGRRIFRFIKGPVFANLVLADEINRTPPKTQAALL
                     QSMQEYRVTAGGTTHDLPLPFLVFATQNPIEQEGTYPLPEAQLDRFMFQVDVDYPSES
                     EEEEIVRQQTSDHRPELVRVLSPQRIMQLQDLVRRVPAADHVIKYAVSLARSTRPTAP
                     NAPAFIRDAVSFGAGPRASQFLILAAKARAILDGRFAVSTEDVAALARPTLQHRLILN
                     YRAEAEGQRAGELVDRLLSVVTP"
     gene            12670..13608
                     /locus_tag="VMZ53_10005"
     CDS             12670..13608
                     /locus_tag="VMZ53_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUS28836.1"
                     /translation="MLDPAQLQRMGTLPLKARVIVEGALSGLHRASVHGSSVEFAEHK
                     EYSPGDELRHVDWKAYAKVDRYYVKQYEQESQLTVYLVLDASASMQFTGGGIAKVEYA
                     GLSLAALAYLVVQQQDKVGLLACGDRAIETLVPPRARTTHLHDLLGVLEQVMQKGGVG
                     DESPAAALRRIAELARRRRALVILASDLFDAADDETLKALAMLRAQRHDVSVLHILDP
                     HERTFPYDGLTEFHALESTNRMLVNPAAIRREYLARMEQFLTKCRVTLANAGVDYHLV
                     QTDRPLEATLLDLLVARSRLSPGRRSTNTRGFAGGP"
     gene            13608..15737
                     /locus_tag="VMZ53_10010"
     CDS             13608..15737
                     /locus_tag="VMZ53_10010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HUS28837.1"
                     /translation="MGFLAPLMLVGAIAVAVPIAIHLIGRRRARVVKFAALDFLMATK
                     RKTAKRLQLRERLLLLVRALVCAAIAIALAKPFTSCDRKGPMVTRGPQAAVIVIDDSF
                     SSGYLVDGKPWLRTAIAEARRVMVQLGPEAEIAIVRTSEGADHPGELTRDHVRVRDQL
                     LAMEPSSRPADTTRALARAAQLLAASSHSRKTVFLFSLLSKQSFHPDEPPWGKDGPQL
                     QTIDLRPSKMANLAITAFRVDADPGAGGRGMAFDAEVGNFSDTAATVELQLSIADRVV
                     ARGSLQVGPGEKKVKRFLATLPAGTRATDASVSLTGDALAIDDRRWVHASLRDDVRVL
                     LVDGDPRTVRHDDELFYLQAALRPGDRDDSGATIRIITPDELSGIDPKTRGKNAPAID
                     IADFDVVLLANVPALPAERVAVLADWVQHGGGIFIAPGDRVDSDAYDRTMLPLMPQSL
                     RDPVDVTWGAPAEERDSRALHLVKWEADHPVFAPFAKDAPLLSDAKFFKISLLGPTTA
                     TADRKVLARFTNGAAALVEASIGAGRTMLFTSTLDTDWNDLPMHPGFLPLMQQAVRHL
                     ARKHGLGGGSEDDHLVGGSVVLPTGDLKRLEVRGPQGLGAVFEGDRITGRSNVRFGRT
                     DHPGVYRVVGTDQTGATHDRDELAFVVNVDPRGSDLSPAPASAIPESGTGGGSAPTDT
                     KRRVELWHALAGLLLLLLLTEGLLVQR"
BASE COUNT         2700 a         5400 c         5070 g         2594 t
ORIGIN      
        1 acgttgagca cgttcaagcc gacgatcggc agcgcgagct cgaacaacgc cttcggaatc
       61 gacttctgtg aaacagcaga taccgccacg tagtggccat cctacgtcat tcggtcgtcg
      121 cgccgaacga cgcaatcacc gtccaaacag gcagggcgat cgttgccgcc gaatcaccgc
      181 gtcgctgcga gtacatgcac gcgatcggcg ctgcaccgaa caatgtgttc acccgcgccc
      241 gctcgaagtt ttcgagcgct cgtcgtatga ttctcatgag ggtcgggcct cgcccgtgcc
      301 gccgcgccgc cgcaccgccg caccgccgcg cagccgcacc cgcaccttcg tgcggccacc
      361 gccggaccgc cgcgcagctc actccgtcgt cgcgccgaac caagccgtga cagtccacac
      421 ggcagcgtca tcactctcgc gatagatgcg tgccgcctgc agcgcgctga acagtcgctt
      481 cgcccacgcg acgcggacct ctcccgcacc tgccgcatcg tcgcgcgcaa tcgcagccca
      541 cgccgccgcc tggaggcgcg ctcccatcgg cgcgcctgca ccgacgacga acagcggccc
      601 gatcgccggt cgctctctcg cctcgagctc gagccatccc gcctccgccc gcgcgccgat
      661 cgcgattccc gcccccacgc cgccgagccc gcgcatcttc gcttcgtcga cgattcccat
      721 acgcccgtca tgcgcgacgc gctcgatccg atacagcggc ccgaacagcg agccgcccgc
      781 gccgcttccg gccctcacat cgccgcgcgc gctccacgtg acgccccact tctcgatctc
      841 gccgctgcca tacgcgagcg ccccagcacc gatcatgatc tccgcggtca cgctaccgcc
      901 gagctccgcg cgcgcgccct ctccttcgaa caagcgctgc gcccccacct cgacgacgcc
      961 gacgctcgcg gcgccgcgcc tctcatcgcc tggatcgatc gctgtcgcga gatgcatgcc
     1021 ccacggcttc gccatctgcc accgcacacc gccgccgacc aaggcgggct cgagcacgtc
     1081 gtcgatctcc aagctcgcat cgagctcatc gccgcggccg ccatcaccgc cgccgcgcgc
     1141 gatggcgcgt acgccggtcc gccagcgatc atcgagcgac acgcgatacc cgtcgacgat
     1201 gcgtcccacg cgcgcaggtg ccagcgcccc ggccgcaacg ccgagttgcg tctcgccgac
     1261 acgaccgctc gcctcgaacg cccgcacgat cgtcaccgcg cgacccgcgc tcgaccaatc
     1321 gctcgcacga aactcgtcat cgtcccagac gccgcgcacg ccgatcccca agcgcgcatg
     1381 cgtccacgcc acatcgagcc tcagctcgac cgacgaatac gtcgatgcgc cctgcgcacc
     1441 cgcgccaacc tgcccgccca caccgacgtc cgcaaccgcg ggcgccgaaa cggcgagacc
     1501 cgcaaatccc gcgaaggcgg cggcgacaca cagcgggcgc atagaccgag cctcgccccg
     1561 ctactaacga agtccaagaa agtggcgagc gctcgcgcgt gagggccgtc gcgcgtggcg
     1621 acgtgggcac aaggctcgtg ctcatcacgc tcgcggatgg gtgagccgcc acaacagcgc
     1681 gagcagtcga tcgagcctcg ccagaacctc gctcgcatcc tcgagccacc cccacgcgac
     1741 cgcggtcaac aacgcggcac gcacttcatt cgcgctgcca tgcgcgaccg catagtgctt
     1801 gcgcttgttg cccttctccg atcgctgtcc ctcgccgaga ttcagcgcga cgctcgatgc
     1861 ggccctcctg atctgatcgg ccaaatctcg atcctgcgcc tcgatcacag gcacgacggg
     1921 cctgagagcc ctgatcacat ctaacgaaac ctcgaatgca atgaacatgc agcctccttc
     1981 gttcttggtc gcgagcaccc gccctgctct cgtccccctt ggggaaccgg ggcccggagc
     2041 ctcgggggca gcgcgggcgg cctctcgccg gtccgcgcga gcgcgccggc gacgtgacgg
     2101 gcgcccactc gcgcgcccgc gatctccgcg cgtgctcacc tccgccgcgc gcgcccgcga
     2161 tctgcgccac cgtctcggcg accggcgcgc cgctggcgca agcgagcgcg agggcagagg
     2221 ccgtccgcgc gtccccgagg cggaggcggt gaatgatgtc gatttcgcgg acccggatca
     2281 gcatccgttg cggcatctga ccccggcgac ggttgcggcg acggctgcgg atgcggctgc
     2341 ggctgcggct gcggctgcgg ctgcggcgac ggatccggat gcggttgcga ctgcggatgc
     2401 ggttgccgcg acggatgcgg ttgccgccag ttgcggcgac ggcgatggcg atagcgtcgg
     2461 ctgcgccgtt acacccgcgg ccgctgcgtc acgctacgcg gcctgcgaat caaccacacc
     2521 atcagcgctc ccatgatcgc gaggatcacg acgccgacgc cgagcagccg ccagcgatat
     2581 gcgccgtgct tcgccggata cggcgatgtc cagaatccag agcggccggg ccgctcttga
     2641 ttcttcatct cctgccactg ctgttgctcg gttggcttcg gcggtggctg ccagggctct
     2701 tgcgagttcg ctgcgcctgg cttcggcggg cccggttgcg cgtacggctt ggcgggcggc
     2761 aactgcgccg atgcgacgtc accacccacc gcgacgaacg cgagaagcac gacgagggcg
     2821 acagcgaggc ggcgcacgcc tcctcttact accttttcga cttctttttc gatttcttct
     2881 tggtgcttgt tggcgcaccc ggcagcggcg gtgcggtcgg ccgcgcgttc tcgatcgtgg
     2941 tcttcacgcg ctcgacgcat tccttctcgg tctccgtctc gacgaggatc atgccatttg
     3001 gcgctttgtg ctcgacctcg tgcgtgttct tggcgaagac gagcggagtt gcgaccgctt
     3061 tgtcgtactc ctcctgcgtc aatcggccgc gcttgagctc gagggcgagg atccggtcga
     3121 tcttggcctg cgtccatttg aagacctggc cctggcagaa ttgtttgtag cggtctttgg
     3181 ggtttggaag gatcgtcgag aagaacgccg cttcgacggg attgagatcc ttcgccgact
     3241 tgccgaagta ctctttggcg gcggggccga tgccgtagag cgcggggccg tactcgatca
     3301 cgttgaagta gatctcgagg attcgatctt tctcgagcgt gttctcgacg tgccacgtca
     3361 ggaacagctc ttggagcttg cgggcgagcg tcttctggcg gaagagcagg acgttcttga
     3421 ccatctgcat cgtgatcgac gatgcgccgt acttgaattt gccggccttg agatcgcgga
     3481 tcagtgcgga cttgaactcc gacacgatga agccgtggtg ttggtagaac gcgccatctt
     3541 cggtcgtcgt gatcgacttg atgagatatg gcgagatgtc ggtgatcgga acgaagtcgg
     3601 ggttctcggg accgacgatg aaagagatcc actcgccttc ttcgacttcg acgaagtgct
     3661 cgaacggctc gatgaggcgc ttcggcgagt ccgccggatc gtcgacgact ttgcagtgct
     3721 tgatgccgac gtggccgtcg agcaccgtgg cgtcgagatt ggtccagtcg atctcgagct
     3781 tgacgtcggt gtcgaacatg cctttgatct tgtagccctt catcgacggc gccatctccg
     3841 cgggaatcgc gtcgatgaag cgttggcagc tgatcggcgg gaccacgagg cgcagcttcg
     3901 cgagctggat gccgtacgga ccgcgcgtag cgacggccag aggctcatcg gcgtcgtcgt
     3961 cgagccggag gtctttcgga tccttcttcg tctgcgcggc ttccggcacg acgacgtcgc
     4021 ggcgcgggcg atagacagtg cccgtgaccg agaacggcac gtcgaacagg aggaagtcac
     4081 cttgcgtgag ctcgagcgtg cgcgtcgcgc gattgaacga gccggcgagg ttcgtcgaca
     4141 ggtcgacgtc gtggacctct ttgtccgcga tcatcgggtg gccgatgttg aggcccgaga
     4201 ggttcatgtc gccggagaat tgggcgacgt cgtgatcgag cgcgacgtgc agctcggcat
     4261 ccaccgacgt cgactggtaa tcgacgacgc ccgacttcgc gaggatcggc gctagacgat
     4321 cgagctggaa cttcgcggcg acgagatcga gcgacgcggt tacctcgtcg acgttgaagc
     4381 cgcctttggc cgtccacagg cgacctggca cgccgccgta gccgcccgcg aagtcgagca
     4441 cgtactcgcc gtgcttggcc ggatccgcga cgatcgtgcc cccgatcccc gacagcgaca
     4501 tcttgggcca cagcgagaac gcgccatcct cgacggtgac gaccggcggc gagcccgctg
     4561 ccttgcggat ctcgatgcct ttgatctccg ccttcggcgc gttcggcgtc gttgcggaga
     4621 cgccgcgcaa gtgcgcgacc gtgacatccg gcgtccacgt cgcatcgccg ctgtcgatca
     4681 gggcggtcgt gccggtgatc gcgtcgtcgg cgagcaggcg gatgcctttg acggtgagct
     4741 tcttcggccg ctgcgacgag ctgccagacg agccgcccga cttcttctca ccgttgccgc
     4801 ggaggcggtc gacgatgtcg cgcacattgt cgtgaccgaa ttgatcgcgg cgcagcgtca
     4861 ccatcatgtc ggtgacgacc gcatcaccga gctcgatcgt gccgaccagg gacgccccgg
     4921 tcgcgaagtc gacatcgatg cgctcgatgt ggacgagcgg catgtcgcca tcgagcttgc
     4981 cgcgcacgtt gacgtcgcgc agcacggcgt gaccgagcga gacatcgatg tcgccgacgg
     5041 tcaccttgcg atcgatgcgc gtcgcgagtc gttcgctgag gcgcttgcgg atcatgtacg
     5101 cgccgacgcg cggatagatc acgccaaggc cgatcagcag gagcagcagc gcaccgaggg
     5161 tgatgccgcc cgcccacatg agccgacgag acaccttcgg aaactgcatg cttccgtata
     5221 caacaaccgg gcgtcgggtt tgtttctact tacaggtcag agcgaggatg tgcccgtcat
     5281 ccttcgcatt cgtcccgacg aggagcagcc aatctccgct cttgatcgcg ggcttggcat
     5341 cgaggaggcg cttgccgtcg gcgccgtccc acgcgaccgt gagctcgacg cgcttctcgg
     5401 tgcgatcgcg cagcatcagc gaggcgccgc cttgcttgag cttgatcgcc tcggccttgt
     5461 tcttcgtcag cgccgtcgta ccggacgaga gcttgtggaa ggtgttccac gcggtgaacg
     5521 gcggcttctt cagcttcttc tcgagaggct ttagatcgcc gtcgatcgcg cccgcctttt
     5581 cgttcgtcgc ggtgatttcg atgaagttgc agtcggcgtc acccgcgaac gcaggcgatg
     5641 ccagcgcagc ggagactacg aatgccgcga gccagatgcg agggctcata tcccctccac
     5701 actatcttct ggagtcaccc agatcacggt tgtgtcgcca tcttcgtctt cgagattgaa
     5761 gacggtcccc gtcccgccag gtgtatcgag cgattgaatg tctgcaggct gatgcacgac
     5821 gggcatcgtg tcgatcggct tacccgtcgg cgtgatcgtc ttgtcggggc tgcgcagcac
     5881 gagcgcgagc attgcgacag ctcctgcgct gacggcgccc gtgatgatgt ggccgcgata
     5941 cgtgtcgaac caccggccaa cgcgccgcaa gaatcccggc gccggtgcag cttctttggt
     6001 cgacgtcgcc ggcgctttct cactcgcacg atcgatccct ttgtcgatct cgcgccacag
     6061 cgcatcgaac ttcttactcg gcaccgcatc ggcggatagc tcgaggtggc cgcgtacgag
     6121 ctcgccgacc tcgcccagtg catccagctt gccgcgtgcg acaggatcgc gtgcgagctc
     6181 ttcttcgagc tcacgcatct gcttttcgtc gagctcgctg tcaccgagca tcatgagatc
     6241 cgcgtccttc atgagcggct ccccgacgca gctgcgtcgt cttcgtcatg cgattcgtcg
     6301 ttggcgaccg cgcgaccttc catgagatcg gcgagtcgct tctgcatgtt ctttcgtgcg
     6361 tggaacaagc gcgacatgat cgtgcccttc gagcagccca tctgttgcgc catttcctcg
     6421 tacgacatgc cttcgagctc ccgcatgacc agcactgctc ggtggttctc cgacagctcg
     6481 ccgagcgcct gatcgatccg agcacggatc tgcttgtcca tcagggccct cccggggttg
     6541 ccaccgagga acttcggcaa tagcgaatcg tcggtggttg ttccgtcgag gtgcgcttcg
     6601 tcgagctcga cgggctttac ccgccgatgc ttgcgaagat ggtcgattgc caggttcatc
     6661 acgattcggt acagccaggt gtagaagctg gagttgcctt cgaacttgtc taagtatttg
     6721 tgagctttaa tgaaagcgtc ttgcactacg tcgagtgcat cgtcttgatg ccgcagcacg
     6781 ccgaatgcca gtgcgtacgc gcgccggtgg taccgctcga acagcgtctt gaacgcgtcg
     6841 cggtcacctt tgcgtgcgga ttcaaccagc tcgcggtcgt cgactgggtc acgtccggtg
     6901 gacgctcgct ggccctcctt attcacgccg ggccggttat atcaccggac cctgcaatgt
     6961 tagatgaacg cggcagggtg tcggatagag tcgcaagcgc tatgctgacg agcgccgggc
     7021 tgtagggcac cccttggctg cgtctcgacc atcgatcccc gggtatttag cccgcgtccg
     7081 tgcgctggtg taccggcacg aggccgcccg agctgcgttt actgcgctcg ctgcgctcgg
     7141 ggcgctcgcg gtcgtgctgc cgctcgtcgg gatcgcgatc tcgaagagcc gcacgacggc
     7201 cctcaccatc ctcagcattg gcggcctcgc cgcgatgctc gtcgtcatcg gcgcgatcgt
     7261 ggtcggcatc gttgcgccgc gccgccgctg gggccgcgat cgcgagctag cgcggtgggt
     7321 cggcacgcgg cagaaatcgg tcgcgagtga cctgctttcg agcgtagagc tcgcagaagc
     7381 gccgccgcgt ccgggatcgc cgtcgaccga tctcgtcgat gcgttgatcg agcagaccgc
     7441 gagcgagctc tcgacgatcg agcccgcaac gctcttgccg gagcgcgagg ttccgcgcgc
     7501 gcgcctgctc gcgctgtttg cgatcgccgt caacgtcgcg ctgctcctgc tcgcgccgag
     7561 cttcatgcac cgcggctggc atcatctcgt cagcgcgccg cgctcaccct tcgacggagc
     7621 cgagctctct aacgttccgc tcgtcggcga cctcgatgcg acgctcacgt ttcccgcgta
     7681 cagcaagcgc gcgaacctcg agctgaagtc gtcgtcggga gactttcgcg gcctgcccgg
     7741 cacgaaggtc cagatctcgg gccacctgct ggtaccggcg acgagcgccg agctcgtgat
     7801 cgagcaaggc aacagcgagc ccaagcgcat cgccgcgaag atcgagaacg agcaactgac
     7861 cgcgcagctg acgatcgagc agagcgcgcg ctatcgcatc gcggtcgaga cgccgagcgg
     7921 cgatcgcaag atcgaagcaa cgccccgcgc gatcgaagcg gagcccgatc aagttcccgc
     7981 ggtgcagctg atggcgccgg cggaccggct cgatgtcgcg aacctgcgcc gcgtcgagct
     8041 cgcatatgtc atcgaggacg acttcggcgt gacgacggcg gagctcgtgt gggaaggcgg
     8101 caaggatcgc ggcaagaagc cgatcactct cggcgagagc gagcagggtc gcgtgcaggg
     8161 caagctcatg tgggacatcg cggagctgca ggtgcagagc ggcggcgagg tccactactg
     8221 gatcgaagcg aaggacaacg acacgatcgg cggcccgaac ctcggcaagt cacgcgagct
     8281 ggtcctgcga gtgacgagcc cgcgcgagcg gcacgaagag acgctcgatc gacagcaggc
     8341 agtcgccgag aagctgctga agaatctcgg cacgcgcctc gtcggcttcg gcgaggacac
     8401 gaccgcccgc gagaccacgt cacacgacct gcgcgattcg atcgtcgagc tatcctcgat
     8461 caacggcgcg tacgaagcgg atccgcacgc ctcggatctg atgcgaaagg cgctcggcgg
     8521 cattcatgat cgcctcgatc ggctcgcgac gatggagagc aagctcctgc cgaagacggc
     8581 gacgaaggta ccgaccggcc gattcgccgc gatcgatccg ccgctcgtgc gcgagctcga
     8641 agacgatctc atcacgctcg ccgactggct cgaccgcgag cgcatggaag gtctgctcga
     8701 tgtcagcgac gagatcgcgg cgcaccagaa gcggctcgcc gacctgctga accagtacaa
     8761 caagacgaag gacgcccgcc tgctcgaaga gatcgaccgc gagatgaagg cgctcgaccg
     8821 cgcgttcgcc gagctcgaga aacaccgcaa aggcatggcc gaggacgtgc tcgaccagta
     8881 cgtcaaccgc gatgcgctcg ccgctgaaga cggaacgaac tgcatggccg aggttcgcgc
     8941 gctcatcgcc gcgggcaaga ccgcggaggc gcagacgaag ctcgagagct gccagcagca
     9001 gcatgcgcgc ggcgccagcg cactcgaggg ctcactcgcg cagctgcgcg gcgacaagtt
     9061 caacgacgag cagaagaagc tcgacgaagt gatgaacgag ctcgccgacc tggcgaaaga
     9121 tcaggacgac atcgcggcgg aagcgaatcg catcttcgag gcgtacgcgc gcaaggccga
     9181 cgatatggca cgggagcacc gcaaagaagc gtcgaagaaa gtcggcgcgt tggtcgaaaa
     9241 gctccgcaag cgcctcggcg cggtgaacga gaccggcctg acgccgttcg cgaaggagga
     9301 gctcgatatc gtcgagcgtc gcgtcgctga cgtcgagcac atggtcgcgg atggcgatct
     9361 cgcggaggcc ctcggcatgg cgcgccaagc gaagacaagc ctcgatacga tcgcgggcga
     9421 gctcgaagcc gcgctcgacg acgatccgaa atcaaagtgg gccgacgcca cgcaagatgc
     9481 gctcgacggc atcgagcaca cggcgccggt cgcgaaggag ctgatcgacg agctgtctgc
     9541 gctctcgcca aagcccgagc agatcatgag cggcgacgat ctcaaggcac tcgatcgtct
     9601 gcgccggcgc gaagcgatga acaagcagcg cacgcagaag ctcggcgatc gcacgaagca
     9661 gatgggccaa gacttgcctg gcgatgccgc ggcggagctc ggcaagaagc tcggcgccgc
     9721 cgccgatcac atgcagaaag ccgacgaccg catgaagggc aaggacccga gcggtgctcg
     9781 cgagtcgacg cgtgcggcag ccgatgcgct cgccaaagcg agagaccgcg ctcgcagcgc
     9841 agctcgccaa gcgcaagagg gctcgatcaa cgacgagccg attcgcattc ccggcgccga
     9901 cgagtacaag gccccggagc ggttccgcga gaacctgctc gaaggcgcga agcaaaagcc
     9961 cgtcgacggc ttcgaggaca tgacgaagcg ctactacgag gagctcgtca aatgaggcgc
    10021 gcgatcgtat tcgcggtgct cctcgtcgcg tcgacggccc acgcgcgtcg cgatcgcacc
    10081 gtcaccggcg acgtgatcaa ggcggctcgc ttcctgacgt cgtcgcgcat cgacgaggcg
    10141 aagacgctgc tcgcggatct cgagaagcgc gcgcccgatg ccgtcgaggt gaagtggctg
    10201 caagccgagc tcgcattcca gaccggcgac tacgccgccg cggtgaagct cctcgacaag
    10261 attcccgaca ccgaggtcga cggcatggtc ggccagacga ggaagctcgc gcagcaatcg
    10321 cttggcgtga ccgagacgtt cgtcgagaag aagtcgccga agggccactt catcgttcgc
    10381 tacgcgcaag gcagcagcga cgccgcgatc gcggacctcg ccggcgaggt gctcgacagc
    10441 acgtgggata cgatcggcga cgacctcggc ctcaagcccg cagatccgat tcgcgtcgag
    10501 atcctcggtg caccggcgga cctcgcgaag ctctcgccgc tgaccgaagc ggagatcgag
    10561 aagaccggca cgatcgcgct gtcgaagtac aacaagctaa tggtcgtgtc gccgcgcgcg
    10621 acgctgttcg gctatccgtg gatggacacg ctcgcgcacg agtacacgca cctcgtggtg
    10681 tcgcgcatcg cgaatgatgc cgtgccggtg tggctgcaag aaggcatcgc tcgtttcgag
    10741 cagactcgct ggcgcaaggc gcccgaagtc gcgctcacgg caaccgagca agcgctcctg
    10801 acgacgggcc tcaagaaggg gcgcttgatc acgttcgacg agatgcaccc gtcgatggcg
    10861 aagctgccga gccaagatgc ggcggcgctc gcgtacgccg aggtctacac gctcgtcggc
    10921 tggatccacg acaagatcgg ctacgagggc atccgcgagc tgctgaccgc gcaaaaggac
    10981 ggcaagtcgg cgcgccgcgc ggtcgccgaa gcgatgggcc agacatggac caagctcgag
    11041 gcggattggc gcgtccacct caaagtctcg agcgacgcga aggtcaaagc cggcaagccg
    11101 atcaagttca aggccggcgg ccaggactcc gagaatgtcg gtctcgacgg cgtgaacgcg
    11161 cgcgctcgca agttcgctcg cctcggcggc atgctgaggg cacgcaacat gctcgaggcc
    11221 gctgccgccg agtacgaaaa agcgctcaac gcgggcaatg acccgttcgt cgcaggcaag
    11281 ctggcccgca ccttggtcga gctgggcaag aacgatcgcg cgatcgagct ggcgacgccg
    11341 ctggcagcgg ccgacgagca tgatgcggtc gccgcagtga cccttggcat cgcccgttcg
    11401 gcaaggcatg aatggaaaga agcgatcgcc gcctatgaac aggcacttcg cgtgagccca
    11461 tttgatccac aaacgcgctg tgggcttgcg gaagcctact cggagatcgg agattctcga
    11521 tctgcgcgag agcggtccgc ctgtgacgag ctgaagaact gaatgaccaa gcccgctgac
    11581 ataacgccga ttcggcaaga agctgcgacc gaggtgaacc ccacggtgcc cgccccgagc
    11641 gacgcgccgc acaagtccga cctcgaagcc gttgccgagc tcgcagacgc gcacaagcag
    11701 atcgtcaccg agatcgagaa gcgcatcatc ggccagcgcc gggtggtcga tcagctgctc
    11761 gtcgccctgt tcgcgcgcgg tcacacgctg ttcgtcggtg tgccgggcct cgcgaagacg
    11821 ctgctcatct cgacgctcgc cgagacgctg aacctgacgt tccagcgcat ccagttcacg
    11881 cccgatctca tgcccgcgga catcacgggc accgaggtcc tcgaagagga tcacacgacg
    11941 ggccgccgca tcttccgctt catcaagggc ccggtgttcg cgaatctcgt gctcgccgac
    12001 gagatcaacc gcacgccgcc gaagacgcaa gccgccctgc tgcagtcgat gcaggagtac
    12061 cgcgtgaccg cgggcggcac gacgcacgac ctgccgctgc cgttcctcgt cttcgccacg
    12121 cagaacccga tcgagcaaga gggtacgtac ccgctgcccg aagcgcagct cgaccgcttc
    12181 atgttccagg tcgacgtcga ctacccgtcg gagtccgagg aagaagagat cgttcgccag
    12241 cagacgagcg atcaccgccc ggagctcgtg cgcgtgctgt cgccgcaacg gatcatgcag
    12301 ctgcaagatc tcgtgcgccg cgtgcccgcc gccgaccacg tgatcaagta cgccgtgtcg
    12361 ctcgcgcggt cgacgcgccc gacggcgccc aacgcacccg cgttcatccg cgacgccgtg
    12421 tcattcggcg cgggcccgcg tgcctcgcag ttcctcatcc tcgccgcgaa ggctcgcgcg
    12481 atcctcgacg gacgcttcgc cgtgtcgacg gaggacgtcg ccgccctcgc ccggccgacg
    12541 ctgcagcacc gcttgatcct caactaccgc gctgaagcgg aaggccaacg cgcgggtgag
    12601 ctcgtcgacc gtctgctctc ggtcgtcacg ccgtaggtcg tcgtgccctc gatgcccgat
    12661 cgaaaaggca tgctggaccc agcgcagctc cagcgcatgg gcacgctgcc gctcaaggca
    12721 cgcgtcatcg tcgagggcgc gctctccggt ttgcatcgcg cgtcggttca cggatcgtcc
    12781 gtcgagttcg ccgagcacaa ggagtactcg ccgggcgacg agctgcgcca cgtcgactgg
    12841 aaggcgtacg cgaaggtcga tcgctactac gtgaagcagt acgagcaaga gtcgcagctg
    12901 acggtctatc tcgtgctcga cgcgtccgcc tcgatgcagt tcaccggcgg cggcatcgcg
    12961 aaggtcgagt acgcgggcct ctcgctcgcc gcgctcgcct acctcgtcgt gcagcagcaa
    13021 gacaaagtcg gcttgctcgc gtgcggtgat cgcgccatcg agactctcgt gccgccgcgc
    13081 gcgcgcacga cgcatctgca cgatctgctc ggcgtcctcg agcaggtgat gcaaaagggc
    13141 ggcgttggcg atgaatcgcc cgcggctgcg ctccgccgca tcgcggagct cgcgcgccgt
    13201 cgccgcgcgc tcgtcattct cgcgtcagat ctgttcgatg ccgccgacga cgagacgctc
    13261 aaagcgctcg cgatgctacg cgcgcagcgg cacgacgtct ccgtcctgca cattctcgat
    13321 ccccacgaac gcacgtttcc gtatgacggg ctaaccgagt tccacgcgct cgagtcgacg
    13381 aaccgcatgc tcgtcaaccc ggccgcgatc cgccgcgagt acctcgcgcg gatggagcag
    13441 ttcttgacga agtgccgcgt cacgctcgcg aatgcaggcg tcgactatca cctcgttcag
    13501 accgatcgcc cgctcgaagc gacgctgctc gatttgctcg tcgcacgctc gcgcctctca
    13561 cccggtcgcc gctcgacgaa cacgcgcggc tttgcgggag ggccctgatg gggtttctcg
    13621 cgccgctgat gctcgtcggt gcgatcgcgg ttgcggtgcc gatcgcgatc catctgatcg
    13681 gccgccgccg cgcacgcgtc gtgaagttcg cggcgctcga cttcttgatg gcgacgaagc
    13741 gcaagacggc gaagcgcctg cagctgcgcg agcgactctt gctgctcgtc cgcgcgctcg
    13801 tgtgcgccgc gatcgccatc gcgctcgcga agccgttcac gtcgtgtgat cgcaagggcc
    13861 cgatggtgac gcgcggtccg caggcagctg tcatcgtcat cgatgactcg ttctcttcgg
    13921 ggtacctcgt cgatggcaag ccgtggctgc gcactgcgat cgcagaggcc cgtcgcgtga
    13981 tggtccagct cggccccgag gcagagatcg cgatcgtgcg gacgagcgag ggcgccgatc
    14041 atccaggcga gctgacgcgc gatcacgtcc gcgtccgcga ccagctgctc gcgatggagc
    14101 cgagctcgcg acccgccgac acgacgcgtg cgctcgcgcg tgccgcgcag ctcctcgccg
    14161 cgtcgagcca ctcgcgcaag acggtgttcc tcttctctct gctgtcgaag cagagctttc
    14221 accccgacga accgccgtgg ggcaaagacg gacctcagct tcaaaccatc gacttgcgcc
    14281 cgagcaagat ggcgaacctc gcgatcaccg cgttccgcgt cgatgccgat ccgggggcgg
    14341 gaggccgcgg catggcgttc gatgccgagg tcggcaactt cagcgacacc gcagcgacgg
    14401 tcgagctcca gctgtcgatc gccgatcgag tggtcgcgcg cggctcgctg caagtgggcc
    14461 ccggcgagaa gaaggtgaag cgcttcctcg cgacgctgcc cgccggcacg cgtgcgaccg
    14521 acgcatccgt ctcgctcacg ggcgacgctc tcgccatcga cgatcgccgc tgggtccatg
    14581 cctcgctgcg cgacgacgtc cgcgtgttgc tcgtcgacgg tgacccgcgc acggtgcggc
    14641 acgacgacga gctgttctat ctgcaagccg cattgcgtcc cggagatcgc gacgacagcg
    14701 gcgcgaccat ccgcatcatc acgccggacg agctctcggg catcgatcca aagacgcgcg
    14761 gcaaaaacgc gccggccatc gacatcgccg acttcgacgt cgtgctcctc gcaaatgtcc
    14821 cggcgctgcc agcggagcgc gtcgcggtgc tcgccgactg ggtgcagcat ggcggcggca
    14881 tcttcatcgc gccgggcgat cgcgtcgact ccgatgcgta cgaccgcacg atgttgccgc
    14941 tgatgccgca gagcctgcgc gatccggtcg acgtcacgtg gggcgcgccc gccgaagagc
    15001 gagacagccg cgcgctgcac ctcgtgaagt gggaagcgga tcaccccgtg ttcgcgccct
    15061 tcgcgaagga cgcgcctctg ctcagcgacg ccaagttctt caagatctcg ctgctcggcc
    15121 cgacgacggc gaccgccgat cgcaaagtcc tcgcgcggtt cacgaacggc gctgctgcac
    15181 tcgtcgaggc gtcgatcggc gccggtcgca cgatgctgtt cacgtcgacg ctcgacaccg
    15241 actggaacga tttgccgatg catcccggct tcctcccgct catgcagcag gcggtgcgtc
    15301 atctcgcgcg caagcacggc ctcggcgggg gcagcgaaga cgatcacctt gtcggtggct
    15361 cggtcgtgct gcccaccggc gatctcaaac gcctcgaggt tcgcggtccg cagggcctcg
    15421 gcgccgtgtt cgaaggcgat cgcatcacgg ggcggagcaa tgttcgcttc ggccgcacgg
    15481 atcacccggg cgtctaccgc gtcgtcggca ccgaccagac cggtgccacg cacgatcgcg
    15541 acgagctcgc attcgtcgtc aacgtcgacc cgcgcggcag cgatctatct cctgcacccg
    15601 cgagcgcgat tcccgagtcg ggcaccggag gcggcagcgc accgacggac acgaagcgac
    15661 gcgtcgagct gtggcatgcg ctcgccgggc tactgctgct gctgttgctg accgagggct
    15721 tgctcgttca gcgctagtga tacgcaggcg tatagcggcc aacg
//
LOCUS       DATLBZ010000190         2331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9117453, whole genome shotgun sequence.
ACCESSION   DATLBZ010000190 DATLBZ010000000
VERSION     DATLBZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9117453"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..636
                     /gene="greB"
                     /locus_tag="VMZ53_10015"
     CDS             109..636
                     /gene="greB"
                     /locus_tag="VMZ53_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HUS28838.1"
                     /translation="MSTRTRSGDRGAPPAAKKSYITPEGAKKLRAELEQLWTVERPRV
                     TQEVADAAAQGDRSENAEYIYGKRRLREIDRRVRFLSKRLDDVTVVTEAPSDPNRVFF
                     GAWVTLEDDEGNEVTYRIVGPDESDIDKGWISMDAPVARALLGKRDGDDVLVRRPKGD
                     ITYTITAIRYSEPSP"
     gene            633..1883
                     /locus_tag="VMZ53_10020"
     CDS             633..1883
                     /locus_tag="VMZ53_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HUS28839.1"
                     /translation="MTTAGRPAPSSPPSRLPDGVPEPIDDAPSRAIAVELPPALRPYA
                     RSPIRWRVGCDVTVLRDGGQTYKAMLDAIVAAKRSICFETYILAGDTTGDRFKIALVE
                     RARAGIEVRLIYDAVGSFGLSDAWVSDLRAAGIEVIAFNPIAPWRQRFRLSHRDHRKM
                     LVVDDEVGFTGGLNIANDYAAVEDGGVGWHDMHCRVTGPIVYDMARGFRRNWIRAGGK
                     TYPPVRRPSSSPTGEGTSYVRLLENALRRQRTGFRRAYLHVMRAAHRTIRIENAYFLP
                     DRGIRRALVAAARRGVDVQVIVPGRSDVKLIEYASLYALRHLAKRGIKVWRWSGIMLH
                     AKTAVVDAIWSTIGSYNFDSQSRFNNLEVTVEILDPVVGEKMVRVFDNDVPNCTPYDE
                     ATWKNLPWWTKSLAWLGYRLRRFL"
     gene            1829..>2331
                     /locus_tag="VMZ53_10025"
     CDS             1829..>2331
                     /locus_tag="VMZ53_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28840.1"
                     /translation="MVDKVARLARLSVATLLVVAACQRAPARIYTDAEIAKLEERVMA
                     KVAEVDASCSHPELLEIDTVLDDACWKAFDVVDRQRRAHTPIVEQNVDTKEIADLDEL
                     CGAKTLEALGRAALTSPGCSPNQVGVRAQSISSPKNEMFARMRVSHVVLFHALREIDP
                     YVALTELL"
BASE COUNT          419 a          805 c          727 g          380 t
ORIGIN      
        1 cgatctcggc cccagcgaac aacccgttgc cgaccaggaa ggccagcacg atcacgatac
       61 tgatgcccat gcgcgacgac gcggagcata cgctatatgc tccgtgcgat gtccacgcgg
      121 acccgcagcg gggatcgcgg cgctccacca gctgcaaaga aaagctacat cacgcccgaa
      181 ggcgcgaaaa agcttcgtgc cgagctggaa cagctctgga cggtcgaacg cccgcgcgtg
      241 acgcaggaag tcgcggatgc ggcggcgcaa ggcgatcgct ccgagaacgc cgagtacatc
      301 tacggcaagc gccggctgcg cgagatcgat cgccgggtgc gctttttgtc caaacggctc
      361 gacgacgtca cggtcgtgac cgaggcgcca agcgatccga atcgcgtgtt cttcggcgcg
      421 tgggtcacgc tcgaggacga cgagggcaac gaggtcacgt accgcatcgt cggccccgac
      481 gagtccgaca tcgacaaagg ctggatcagc atggatgcgc cggtcgcccg cgcgctcctc
      541 ggcaaacgcg acggagacga cgtcctcgtt cgccgcccca aaggcgatat cacgtacacg
      601 atcaccgcca tccgctactc ggagccttcg ccgtgactac cgccggccgc cccgcccctt
      661 cgtcgccgcc gtcgcgcctt cccgacggcg ttcccgagcc gatcgacgac gcgccgtctc
      721 gcgcgattgc cgtcgagctg cctccagccc tgcgcccgta cgcccgctct cccattcgct
      781 ggcgcgtcgg ctgcgacgtc accgtcctgc gggacggcgg ccaaacctac aaggcgatgc
      841 tcgacgcgat cgtcgccgcg aagcgttcga tctgcttcga gacgtacatc ctcgcgggtg
      901 acaccacggg cgatcgcttc aagatcgcgc tcgtcgaacg cgcgcgcgca ggcatcgaag
      961 ttcgcctcat ctacgacgcc gtcggttcgt tcggcctctc cgacgcatgg gtgagtgacc
     1021 tgcgtgccgc cggcatcgag gtcatcgcat tcaatccgat cgcgccatgg cgccagcgct
     1081 tccggctctc gcatcgcgac caccgcaaga tgctcgtcgt cgacgacgag gtcggcttca
     1141 ccggtggcct caacatcgca aacgactacg cggccgtcga agatggcggt gtcggctggc
     1201 acgacatgca ctgccgcgtg accggcccga tcgtctacga catggcgcgc ggcttccgcc
     1261 gcaactggat ccgcgcaggc ggcaagacgt atcccccggt ccgccgcccc tcctcttcgc
     1321 cgacgggcga aggcacgtcc tacgttcgcc tgctcgagaa cgcgctgcgc cgccagcgca
     1381 ccggctttcg ccgcgcgtac ctccacgtga tgcgcgccgc gcaccgcacg atccggatcg
     1441 agaacgccta cttccttccc gatcgcggca tccgccgcgc gctcgtcgcc gccgcccggc
     1501 gaggcgtcga tgtccaagtc atcgtgcccg ggcgctcgga cgtgaagctc atcgagtacg
     1561 cgtcgctcta cgcgctgcgc cacctcgcaa agcgcggcat caaggtctgg cgctggagcg
     1621 gcatcatgct tcacgcgaag accgcggtcg tcgatgcgat ctggtcgacg atcggcagct
     1681 acaacttcga ctcgcagtcg cgcttcaaca acctcgaggt gacggtcgag attctagatc
     1741 ccgtcgtcgg cgagaagatg gtgcgcgtgt tcgacaacga cgtgccgaat tgcacgccgt
     1801 acgacgaggc gacatggaag aatctcccgt ggtggacaaa gtcgctcgcc tggctcggct
     1861 atcggttgcg acgcttcttg tagttgccgc atgccagcgc gcgccggcgc ggatctacac
     1921 ggacgcggag atcgcgaagc tcgaagagcg cgtgatggcg aaggttgcgg aggtagatgc
     1981 gagctgctcg caccccgagc tattggagat cgatacggtg ctcgacgacg cgtgttggaa
     2041 agcgttcgac gttgtcgaca ggcaaaggcg agcgcacacg cccatcgtcg agcagaacgt
     2101 cgatacgaag gagatcgcgg acctcgacga gctctgcggt gcgaagacgc tcgaagcact
     2161 cgggcgcgcg gcactaacat caccgggatg ctctccaaat caggttggcg tacgcgcgca
     2221 gtccatctcc tcgccgaaga acgagatgtt tgcgcgtatg cgcgtaagcc atgtcgtgct
     2281 gtttcatgcg ctacgcgaaa tcgatcccta cgtcgcgctc acggagcttc t
//
LOCUS       DATLBZ010000191         5636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5398451, whole genome shotgun sequence.
ACCESSION   DATLBZ010000191 DATLBZ010000000
VERSION     DATLBZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5398451"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..236)
                     /locus_tag="VMZ53_10030"
     CDS             complement(78..236)
                     /locus_tag="VMZ53_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28841.1"
                     /translation="MKRASRIQQDPRKRIERQRRLLRDAGEQLEVDDAELEEIDTELA
                     RRMLELES"
     gene            complement(233..1417)
                     /locus_tag="VMZ53_10035"
     CDS             complement(233..1417)
                     /locus_tag="VMZ53_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HUS28842.1"
                     /translation="MTIERSIIEAATRLRRKGESHLIATVVRTQGSAYRGPGARMLLT
                     HFRWLTGSVSGGCLEGDIASKGWWRTRDGEPVVVTYDSRTPEHADDDDVRSAFGLGCD
                     GVVEVMLERGNTPGRIDPLEVADRCYRTQKRGAVATVIHSRVNGVKIGHRVAVLVGSD
                     AQADASLDDVLRIAMAADCKAAIASGESVIRTYNSERGSVDVFVEAILPPPRVFIFGT
                     GHDVVPLVSILKTLGWDISVCTSQPRVSTRQRFIGVADELLVGTRAEIAARIDECDCA
                     VALVMSHNYETDRENVGMLLGTSARYIGVLGPRNRTAKLFSDLQVQPDSRVHAPVGLQ
                     LGAETPQEIALAIAAEVQSVLAKAPASSLRDRIGPIHDRPRMSRISEAFHAISPITAE
                     AS"
     gene            complement(1483..2541)
                     /locus_tag="VMZ53_10040"
     CDS             complement(1483..2541)
                     /locus_tag="VMZ53_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28843.1"
                     /translation="MTTALVGCAGNGMTGDDMTGDDDGSGGSRPTMDTAVATLAGWAQ
                     PGYLDGDRQKNLFNNPTNAVYGPDGKLYVADFDNGKIRVVDMDGKSSTLIAKEGFQRP
                     FGLAFIGSTLYVGTDRDPQGVGGLSDLMSGTIWKVDVTARTATPVATRIGRPRGLCAL
                     SDGTLGVTDYAHHVVQVFDPGSGSISPIAGSWDAKGFADGNGASAQFATPYACAQTSD
                     GKLLVTDFDNHRVRLVGLDGSVSTIAGSGSAGFADGSGASAKFNHPQGLVKTSGGFYI
                     TDIDNYRVRKISNDLSSISTLAGDGTPGYKDSDDKATAQFYGLEGLTAKADGSLIFVA
                     DGTRGEDVPYNRIRLIKN"
     gene            complement(2662..3198)
                     /locus_tag="VMZ53_10045"
     CDS             complement(2662..3198)
                     /locus_tag="VMZ53_10045"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HUS28844.1"
                     /translation="MKRAVIAIVDAAQARLFTYEEEGADPAQQLHEIKDLANPGRKLR
                     IAEQVSNSEPGRASASDGPALPMHGGVTHTRGYERSATDDHRFAKEAEMDAKFARVVV
                     DALNEIINRDGFRSVILAAGPKMLGELRQQDGVLRRPDVEIHEIPKTLASLSVAQLHD
                     HLAALDLVPPRRRLSAAR"
     gene            complement(3257..3883)
                     /locus_tag="VMZ53_10050"
     CDS             complement(3257..3883)
                     /locus_tag="VMZ53_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28845.1"
                     /translation="MRALIILLLLCATASAEPSRERLFWTGAAMAPPTYLIGVSLHEG
                     SHAIAAKLVGGTVDEVHLFPPGVDPKVHKFRFGWVYARGLKTRGDRILFYLAPKITDS
                     LLLAGYTALLFTAFPDNKYGQLALTVGATGLWVDFSKDLVLFSPHNDVVKGFRGWCMY
                     GIKQLPARLVYATAVAGFGYVVARGYQKTFERQDATTMMTMPLVSGTF"
     gene            complement(3871..4503)
                     /locus_tag="VMZ53_10055"
     CDS             complement(3871..4503)
                     /locus_tag="VMZ53_10055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS28846.1"
                     /translation="MTLPDSPRWVEANGIAGDPAHWRAELGRGGYALGHDAAKLIVIV
                     GEPEAKALAALVVRHPQHVLLLENPAKAPRPAQRAILHTLPDPDALPDYEGAVPLPVD
                     AALPASLEVELTWARTRATIWTAYVDGAPACFAYAPWRSAALFDVSVDTLPSARQLGL
                     ATITAAAMIRDERAQGREPVWGADEGNAASLRLAARLGFVATDEIWVCAP"
     gene            complement(4500..>5636)
                     /locus_tag="VMZ53_10060"
     CDS             complement(4500..>5636)
                     /locus_tag="VMZ53_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28847.1"
                     /translation="ERPGRDTGSQPLVSDTGQLNLDDNSYAITDAIEEALAVAPNTPS
                     RATPSQGTKQKQSSLPLPHEATKVGVPPVAPTEPAPPKVGGGQPLGRRQPQAGGGVGL
                     GLPYTGPSGEPVFGLTPQQRLAQPADARKKKYMLYGGIAGVAIVIGIVIAVVTAGPKK
                     TGLDRNTPAGQAAEAMDRGDYNGAIKILEDKKDVIQNDADAQLVLGRAYYARGDLPLA
                     LGAYRLALTIRPEKESDRDLRASLRAMAVSKDPEILTNVFDIWVGETKDPAAQEALFK
                     AAVSSNIERRHAALVVLDHRKLGDTKLRIQSYSLDLQDGPTCPARAEAVGKLQAIGDP
                     AAIPVLERAIKRRVTSGKLAGKPINACLEEDAKAAIGYLNGLKK"
BASE COUNT         1002 a         1951 c         1719 g          964 t
ORIGIN      
        1 cgcggcggcg gtgccgacga caccgaacgc agcgaacttg gcagcaatgg caaacagcgc
       61 gatggaaagg acctttttca tgactccagc tccagcatgc gacgcgccag ctccgtatcg
      121 atctcctcga gctccgcgtc atcgacctcg agttgttcgc ccgcatcacg caggagccgg
      181 cgctgacgct cgatgcgctt gcgtggatcc tgctggatgc gagatgcccg tttcatgacg
      241 cctccgccgt gatcggcgag atggcgtgga atgcttcgct gatccgcgac atgcgcggac
      301 ggtcgtgaat cggcccgatg cggtcgcgca ggctcgacgc gggcgccttc gcgaggaccg
      361 actggacctc cgcggcaatc gcgagcgcga tctcctgcgg cgtctccgct ccgagctgga
      421 ggcccaccgg cgcgtgaacg cgcgagtcag gctggacctg gagatccgag aacagcttcg
      481 cggtgcgatt gcgcggaccg aggacgccga tgtagcgagc gctcgtgccg agcagcatgc
      541 cgacgttctc gcgatccgtc tcgtagttgt ggctcatcac cagcgcgaca gcgcaatcgc
      601 actcgtcgat gcgagccgca atctcggcac gcgttccgac gagcagctcg tccgcaacac
      661 cgataaaccg ctgccgcgtc gacacacgcg gttggctcgt gcagaccgag atgtcccaac
      721 cgagcgtctt caggatcgag acgagcggca cgacatcgtg gccggtcccg aagatgaaca
      781 cgcgcggcgg cggcaggatc gcctcgacaa acacgtcgac gcttccacgc tccgagttgt
      841 acgtccggat cacggactct ccgctcgcaa tggccgcctt gcagtcagcc gccatcgcga
      901 tgcgcaacac gtcgtcgagc gacgcatcgg cttgggcatc actgccgacg agaaccgcga
      961 cgcgatggcc gatcttcacg ccgttgacgc gcgagtggat gaccgtcgcc acggcgccgc
     1021 gcttctgcgt gcgatagcag cggtccgcaa cctcgagcgg atcgatgcgg ccgggcgtgt
     1081 tgccacgctc gagcatcact tcgacgacgc catcgcagcc gaggccgaac gccgaccgaa
     1141 cgtcgtcatc gtcggcgtgc tcgggcgtac gcgagtcgta ggtcacgaca acgggctcgc
     1201 catcgcgcgt ccgccaccag cccttgctcg cgatatctcc ctcgaggcag ccgccgctca
     1261 cggacccggt cagccagcgg aagtgcgtca gcagcatccg tgcacccggg ccgcggtacg
     1321 ccgagccttg cgtgcggacg accgtcgcga tcaagtgcga ctcgcccttc cgacgcaggc
     1381 gcgtcgcggc ttcgatgatg gagcgttcga tggtcattgg aggaggtcct tgacgacaga
     1441 cactgcaaac cgcgggcgag gcccgaatcg atctcagcga gctcagttct tgatgaggcg
     1501 gatgcggttg taaggaacgt cctcaccgcg cgtgccgtcc gcgacgaaga tgagcgagcc
     1561 gtccgccttc gccgtcaggc cttcgaggcc gtagaactgt gccgtcgcct tgtcgtcgct
     1621 gtccttgtag ccgggcgtgc cgtcacccgc gagcgtcgag atgctcgaga ggtcattgct
     1681 gatcttgcgg acgcgatagt tgtcgatgtc cgtgatgtag aagccgcccg acgtcttcac
     1741 gagaccctgc gggtggttga acttcgcgct cgcgcccgag ccgtcagcaa agcccgcgct
     1801 gcccgagcca gcgatcgtcg acaccgagcc gtcgagtccg acgaggcgaa cgcgatggtt
     1861 gtcgaagtcc gtcacgagca gcttgccgtc gctcgtctgc gcgcaggcgt acggcgtagc
     1921 aaactgtgcc gaggcgccgt tgccatccgc aaagcccttc gcatcccacg agccggcgat
     1981 cggcgagatc gagcccgagc ccgggtcgaa gacctgcacg acatggtgcg cgtaatccgt
     2041 gacgccgagc gttccgtcgc tcagtgcaca gaggccacgc gggcgaccga tgcgcgtcgc
     2101 aaccggcgtc gccgtacgtg ccgtgacatc gaccttccag atcgtgcccg acatcagatc
     2161 gctcagacca ccgacgccct gcgggtcgcg gtcggtgccg acatagagcg tgctgccgat
     2221 gaacgcgaga ccgaacggac gctggaagcc ttccttcgcg atcagcgtgg acgacttgcc
     2281 gtccatgtcg acgacgcgaa tcttgccgtt gtcgaagtca gcgacgtaga gcttcccgtc
     2341 cgggccgtac acggcgttcg tcgggttgtt gaacaagttc ttttgacgat caccgtcgag
     2401 ataaccgggc tgcgcccagc cggcgagcgt cgccacggcc gtgtccatcg tcggacgcga
     2461 gccgccgctg ccgtcgtcgt ctcccgtcat gtcatcgcca gtcatgccgt tgccggcaca
     2521 accaacgaga gcagtcgtca tcgacagaga aagtaggagg ttcgttctcg acatgacggg
     2581 ttcagtgagc aaaccgcttg ccagacgaga atctgcgccg cgacgcacag atgacggctc
     2641 attgtcgcgt gcgccgatga ctcagcgagc cgccgagagg cgacgccgag gaggaaccag
     2701 gtctaacgca gccaaatgat cgtgcagctg cgccacgctg agcgacgcca aggtctttgg
     2761 aatctcgtga atctcgacgt ctgggcgtcg gagcacacca tcttgctggc gcagctcgcc
     2821 gagcatcttc ggccctgccg ccaaaatcac acttcgaaac ccatcccggt tgatgatctc
     2881 gttgagcgca tcgaccacaa cgcgcgcgaa tttcgcgtcc atctctgctt ccttcgcgaa
     2941 gcgatgatca tcggtcgccg agcgctcgta gcctcgcgtg tgcgtcacgc cgccatgcat
     3001 gggcaacgcg ggcccatccg acgccgacgc gcgtccaggc tcgctgttcg agacctgctc
     3061 cgcgatgcgc agcttccgcc ccgggttcgc gaggtctttg atctcgtgaa gctgctgcgc
     3121 aggatcagct ccctcttctt cataggtaaa caaacgcgcc tgtgccgcgt cgacgatggc
     3181 aataacagct cttttcatga cagcgtctcc ctcgcgtctt gcatctacgc aagttgcaag
     3241 tgaggtgccc gcagtgtcag aacgtgccgg acacgagcgg catcgtcatc atcgtcgtgg
     3301 cgtcctgccg ctcgaacgtt ttctggtacc cacgcgcgac gacataacca aagcccgcaa
     3361 cagccgtcgc atacaccaag cgtgcaggca gctgcttgat gccgtacatg caccaaccgc
     3421 ggaatccctt cacgacatcg ttgtgcggcg aaaacagcac gaggtccttc gagaagtcga
     3481 cccacaagcc ggtcgctcca acggtgagcg cgagctggcc atacttgttg tcgggaaacg
     3541 ccgtgaacag cagcgcggtg tagccggcga gcagaagtga gtcggtaatc ttgggcgcga
     3601 gataaaatag aatccgatcg ccgcgcgtct tcagccctcg cgcgtacacc catccgaagc
     3661 gaaacttgtg gaccttcgga tcgacgccgg gcggaaacag atgcacttcg tcgaccgtgc
     3721 cgcccacgag cttcgccgcg atcgcgtgac tgccctcgtg cagcgagacg ccgatcaaat
     3781 acgtcggcgg tgccatcgcc gcacccgtcc aaaacaatcg ctcgcggctc ggctcggcgc
     3841 tcgccgtcgc acacagcaga agaagaatga tcagggcgcg catacccaga tctcgtcagt
     3901 ggcgacaaag ccgaggcgcg cagcgagccg caacgacgcc gcattccctt catcggcgcc
     3961 ccacacgggc tcgcgtcctt gcgcgcgctc gtcgcggatc atcgccgcag cagtaatcgt
     4021 cgcgaggccg agctgccgcg cagaaggcag cgtatcgaca ctgacgtcga acagcgccgc
     4081 gctccgccac ggtgcatacg caaagcaagc cggcgcgccg tcgacgtacg cggtccaaat
     4141 cgtcgcgcgc gtccgtgccc acgtgagctc gacttccaag ctcgccggca gcgctgcatc
     4201 cacaggaagc ggaacggcgc cctcgtagtc gggcagcgcg tctggatcag gcagcgtgtg
     4261 caagatcgcg cgctgcgcag ggcggggcgc cttcgcagga ttctcgagaa gcagcacgtg
     4321 ctgcggatgc ctcacaacaa gcgccgccag cgccttcgcc tccggctcgc cgacgatcac
     4381 gatgagcttg gccgcatcat gcccgagcgc atagccgccg cgaccgagct ccgcgcgcca
     4441 gtgcgccggg tcgccggcaa tcccattcgc ctcgacccac cgtggcgaat ccggcagcgt
     4501 cacttcttca gcccattcaa atagccaatc gcggccttcg catcttcttc gaggcaggca
     4561 ttgatgggct tgccggcgag cttgccactc gtcacgcgtc gtttgatcgc gcgctcgagc
     4621 acaggaatcg cggccggatc gccgatcgcc tgcagcttgc ccaccgcctc cgcgcgcgca
     4681 ggacacgtcg gcccgtcctg cagatcgagc gagtagctct gaattcgcag cttcgtatcg
     4741 ccgagcttgc gatggtccag cacgacgagc gccgcatgcc gcctctcgat gttcgacgac
     4801 acggccgcct tgaacagcgc ctcctgcgcc gcaggatctt tcgtctcgcc cacccagata
     4861 tcgaacacgt tcgtcagaat ctccggatct ttcgacaccg ccatcgcacg cagtgacgcg
     4921 cgcaaatcgc ggtcgctctc cttctcgggc cgaatcgtca acgcgagtcg ataggcaccg
     4981 agcgcgagtg gcaggtcgcc gcgcgcgtaa tacgcacggc ccaacacgag ctgcgcatcc
     5041 gcatcgttct ggatgacgtc cttcttgtcc tccagaatct tgatcgcacc attgtagtcg
     5101 ccgcgatcca tcgcctccgc cgcctgaccc gcgggcgtat tgcgatccaa tcccgtcttc
     5161 ttcggccccg ccgtcacaac cgcgatgacg atgccaatga cgatcgccac accggcgatg
     5221 ccgccataca gcatgtactt cttcttccgc gcatccgccg gctgcgccag tcgctgctgc
     5281 ggcgtcagcc caaagacggg ctcgccgctc gggccggtat acggcagccc caatccaacg
     5341 ccgccccccg cttgcggttg ccgccgcccc aacggctgcc caccaccgac cttgggcggc
     5401 gcaggttcgg taggcgcaac cggcggcacg cccactttcg tcgcctcgtg cggcagtggc
     5461 aacgacgatt gcttctgctt cgtcccctgc gaaggcgtgg cgcgcgacgg cgtattcggc
     5521 gctaccgcca gcgcctcttc gatcgcatcg gtaatcgcgt agctgttgtc gtcgaggttg
     5581 agctgccccg tatcactaac cagcggctgg cttccggtat cacgccccgg tcgctc
//
LOCUS       DATLBZ010000192         6927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_185792, whole genome shotgun sequence.
ACCESSION   DATLBZ010000192 DATLBZ010000000
VERSION     DATLBZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185792"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /gene="ygiD"
                     /locus_tag="VMZ53_10065"
     CDS             complement(<1..806)
                     /gene="ygiD"
                     /locus_tag="VMZ53_10065"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HUS28848.1"
                     /translation="METSTATSCMPAAFIGHGSPTNTLEDNRFTQSWRAFGAAVPRPR
                     AVLVVSAHWYAAVTAVTAMVRPRTIHDLFGFGERLQTVQYPAPGAPDIAKEIRDVVKP
                     RFVGLDQDAWGIDHGAWSMLAHVFPQADVPVIQLSINGTKDFAYHLDIGERLAPLRER
                     GILIIGSGNIVHNLGRIDWARRDGAFDWAVRFDEAAREIMTSRPEDVLSLQKHPDFES
                     AVPTPDHFIPLLYLAGLAKHCKRTPRILVEGYALGSISMTSYTLDCECPGG"
     gene            905..4558
                     /gene="hrpA"
                     /locus_tag="VMZ53_10070"
     CDS             905..4558
                     /gene="hrpA"
                     /locus_tag="VMZ53_10070"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HUS28849.1"
                     /translation="MSGDIQLDPRVVFPDLPITERVPAIAEAIRDHQVVIVAGETGSG
                     KTTQLPKICLAMGRGIDARIGCTQPRRIAATSVAARVAQELDTQLGAVVGYKIRFSDK
                     LGQSSRVKFMTDGILLAEIQSDPLLRQYDTLIIDEAHERSLNIDFLLGFLKQLLPRRR
                     DLRVVISSATLEIERFQKFFGGAPVVEVSGRTYPVDVLYRPPTEDEADLAELVANAVN
                     EIAEMDPHGDVLVFLPGEREIREAMGELEQRALPHTELLPLYGRLAAADQQRVFSRGT
                     GRRVVLATNVAETSLTIPGIVYVVDSGVARINRYEPRSGVSQLLVEDVSRASADQRKG
                     RCGRTQNGICVRLYDEKNYLARDAYTDPEIKRVSLAGVILRMKSLRLGDISAFPFLDP
                     PDKRAINDGYRVLEELGAIDEEYNLTQIGVQLSKLPLDPRLARMILGGRDEHALREVV
                     IIAAALGLRDPRDRPQAAQQQADEAHRAWRDEGSDFVSYLKMWSFWKDAQESRSRRAL
                     QKLCRDKFLSYMRLREWDDIHDQIVRVMKEMEWAPNAQPASGEQIHRALLPGLLSRIG
                     MYNKEARNYFGARQTRFTIHPSSGIAKKPPAWVMAAELVETSQLFARTCAKIEPAWLE
                     RAGGDLCKRNYGDPHWEQRSAQVMVKENTTLFGLPIVRDRSVTYTDRNPSLCREMFIT
                     HALVRHEYTSKGAFMAHNARVLADAQRMRDKARRSDMFADDDQVAQFFDARLPPSVVS
                     GKTFEQWRADAERRDPKILFLSLADILLGEPDLTTARYPDQLEIGGVPLHLSYRFEPG
                     EDDDGITITVPLAVLPQLDPDVMEWTIPGWHQPKILALLESLPKSLRKKLQPLDLLAT
                     ELAAAVQPFSGPMLPALARAIYQKTGDRITPDQWDLRAVPRYLDFSYRVIDDHDKPLA
                     ASKDLRDLQDQLGNRARALWTASPREAIERSQLRTFPDALPETVTVAINGRKVQAFPA
                     LVDREHHVDLKTLESAPAALQATREGLRRLYLFDSGNSFSRLDAQMHAPLGDRRALVM
                     RALDEAFHLTAPPPRTRADYERRLELGCNALPIEVARSGRIASELSAELTPIKAALQP
                     LVGKPGVAKIFVDDVQSQLAHLAPPDLYQRTGFDRLEAIVRYLKALRIRIQRQAVDPM
                     KDQQKATHVVPLWQQYIAQRPALLAKGRTEKELDEFSWLVEELRVQIFAPEVKPALSI
                     NLARVQDAWAKLR"
     gene            complement(4570..6276)
                     /locus_tag="VMZ53_10075"
     CDS             complement(4570..6276)
                     /locus_tag="VMZ53_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28850.1"
                     /translation="MPQEAGAWSIGSQLNYEGCHERITATAFRNARALYMTAPRIVPN
                     REEAGLIGEVQFVPPSDFVDDLAPIALLLGVRDSDLKGHNPLNTLSLVGVHGDPITQE
                     EHCIRAETDDGDAGNESALAACRRFIVRRATEALDGLTASGEVDPNIRVPLPVYVSFT
                     GHVKPELPLFYIRMGQAVHAVEDGFTHTYRTSDGALVTVVMNWIDNTSGTAATELTDG
                     PVHLAPMDHCESPDPLVARNQQLAIEASTAFLAASLDPAMTREQKIATFETLAIKYFS
                     YQPGCTFDNNYCDAPEPSVAAVGCDAGRQGRSWSTALFVIAAVLLARRRRNHIVGVAA
                     ITLTLAALPAHADTTAPTAPTAPTDPATATPPAAASEPAVPITSTEPGDAASVREGTE
                     PGRDASSLTVGELSEVREDKRLGSRIGVSAMLGGSIVHAAADISIGARYRLNENWLIG
                     LDAEWNPWFTAVPITMKRGVASVYATLVRRFPMAYNRVNLRTSVHVGVSTLLFDVNGA
                     PMYSMGPFVSFTPLGIDYDLGGAVRLVLDPVEIAVPAPNLGLIPLYYEQFRLMIGLQI
                     GA"
     gene            6665..>6927
                     /locus_tag="VMZ53_10080"
     CDS             6665..>6927
                     /locus_tag="VMZ53_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUS28851.1"
                     /translation="MNTRLYVGNLSFNTTADGVRTAFEQFGTVTDVHLVTDRETGRAR
                     GFAFVTMSTPDEAAKAMQSMDGQTLDGRPLRVNEAEQRQQRGGG"
BASE COUNT         1258 a         2167 c         2310 g         1192 t
ORIGIN      
        1 ccgcccgggc actcgcaatc gagcgtgtac gacgtcatcg aaatggatcc gagtgcgtaa
       61 ccctcgacga ggatgcgtgg ggtgcgcttg cagtgtttcg cgagaccggc gaggtagagc
      121 agcggaatga agtgatcggg cgtcggcacc gcgctctcga agtcgggatg tttctggagc
      181 gagagcacgt cctcgggtcg ggacgtcatg atctcccgcg ccgcttcgtc gaaccggacg
      241 gcccaatcaa atgcaccatc gcggcgcgcc caatcgatgc ggccgaggtt gtggacgatg
      301 ttgccactgc cgatgatcag gatcccgcgt tcgcgcagcg gagcgagacg ctcgccgata
      361 tcgaggtgat atgcgaagtc cttcgttccg ttgatcgata gctggatcac cgggacgtcg
      421 gcttgcggaa acacgtgcgc gagcatgctc cacgcgccgt ggtcgatgcc ccacgcgtcc
      481 tggtcgagcc cgacgaaccg cggcttgacg acgtcgcgga tctctttcgc gatgtcaggc
      541 gcacccgggg ccggatactg gaccgtctgc agtcgctcgc cgaagccgaa gagatcgtgg
      601 atcgtgcgtg ggcgcaccat cgcggtgacc gcagtgacgg cggcgtacca gtgtgcagag
      661 acgacgagca cagctcgcgg tcttggcact gccgctccga aggcgcgcca cgattgtgtg
      721 aagcggttgt cctccagcgt gttcgtcggg cttccgtgcc cgatgaatgc tgccggcatg
      781 caactcgtgg cggtgctcgt ctccatcgcg agacgctacc cgcgagaaga aagggacgcc
      841 agacccgttg gataaaggcg tgttgtagcg cgacgggacg agccttcgcg gtaaacaagc
      901 gctcgtgagc ggggatatcc aactcgatcc ccgcgtcgtg tttccagatc tgccgatcac
      961 ggagcgcgtc cccgcgatcg cggaagcgat tcgcgatcac caggtcgtca tcgtcgcggg
     1021 cgagaccgga tctggaaaga ccacgcagct accgaagatc tgcctcgcga tgggccgcgg
     1081 gatcgatgca cgcatcggtt gcacgcagcc gcgccggatc gcggcgacca gtgtcgcggc
     1141 acgcgtggcg caggagctcg acacgcaact cggtgcggtc gtgggctaca agatccggtt
     1201 ttcggacaag ctcggccagt cgtcacgcgt gaagttcatg acggacggca tcctgctcgc
     1261 ggagatccag agcgatcctc tgctgcgcca gtacgacacg ctgatcatcg acgaagcgca
     1321 tgaacgctcg ctgaacatcg acttcctgct cggcttcctg aagcagctgt tgccgcgccg
     1381 ccgcgacctg cgcgtcgtga tcagctcggc cacgctcgag atcgagcggt tccagaagtt
     1441 cttcggcggt gcaccggtcg tcgaggtgtc gggacggacg tatcccgtcg acgtgttgta
     1501 tcggccgccg acggaagacg aggcggatct cgcggagctc gtcgcgaacg cggtcaacga
     1561 gatcgccgag atggatccac acggcgacgt gctcgtgttc ctgcccggtg agcgcgagat
     1621 ccgcgaggcg atgggcgagc tcgagcaacg cgcattgccg cataccgagc tgctgccgct
     1681 atacggacgg ctcgccgcgg ccgatcaaca gcgcgtgttc tcgcgcggta cgggacgtcg
     1741 cgtcgtgctc gccacgaatg tcgccgagac gtcgctgacc attccgggca tcgtctacgt
     1801 cgtcgacagc ggcgtcgcgc gcatcaatcg ctacgagccg cgctcgggcg tgagtcagct
     1861 gctggtcgag gacgtctcgc gagcgagtgc agatcagcgc aagggccggt gcgggcgcac
     1921 gcaaaacggc atctgcgtgc gactgtacga cgagaagaac tatctcgcgc gcgacgcata
     1981 caccgatccg gagatcaaac gagtgtcgct tgcgggcgtg atcctgcgga tgaagtcgct
     2041 gcggctcggt gacatcagcg cgtttccatt cctggatccg ccggacaagc gcgcgatcaa
     2101 cgacggctat cgcgtgctcg aagagctcgg tgcgatcgac gaagagtaca acctgacgca
     2161 gatcggcgtt cagctgtcga agctgccgct cgatccgcgg ttggcgcgga tgatcctcgg
     2221 cggccgcgac gagcacgcgt tgcgcgaggt cgtgatcatc gccgcggcgt tgggccttcg
     2281 cgatccgcgc gatcgtccgc aggccgcgca gcagcaagcg gatgaagcgc atcgcgcgtg
     2341 gcgcgacgag ggcagtgact tcgtcagcta cctgaagatg tggtcgttct ggaaggatgc
     2401 gcaggagtca cgctcgcgcc gcgcgctgca gaaactgtgt cgcgacaagt tcctctcgta
     2461 catgcgtctt cgcgagtggg acgacatcca cgatcagatc gtgcgcgtca tgaaggagat
     2521 ggaatgggcg ccgaacgcgc agccggcatc gggtgaacag atccatcggg cgctgttgcc
     2581 ggggctactc agccgcatcg ggatgtacaa caaggaggcg cgcaactact tcggggcgcg
     2641 ccagacccga ttcacgatcc atccgtcgtc ggggatcgcg aagaagccgc cggcgtgggt
     2701 gatggccgcc gagctcgtcg agacgtcgca gctgttcgca cggacgtgcg cgaagatcga
     2761 gccggcgtgg ctcgagcgcg cgggcggcga cctgtgcaag cgcaactacg gcgatccgca
     2821 ttgggagcag cgcagcgcgc aagtgatggt gaaagagaac accacgctct tcggcctgcc
     2881 gatcgtgcgc gatcggtcgg tcacgtacac cgaccgcaac ccttcgctgt gtcgcgagat
     2941 gttcatcacc cacgcgctcg ttcgccacga gtacacgagc aagggagcat tcatggctca
     3001 caacgcacgc gtgctcgccg atgcgcagcg gatgcgcgac aaggcgcgcc gcagcgatat
     3061 gtttgccgac gacgaccaag tggcgcagtt cttcgatgcg cggttgccac ccagcgtggt
     3121 ctcgggcaag acgttcgagc agtggcgcgc cgatgccgag agacgcgatc cgaaaatctt
     3181 gtttctctcg cttgccgaca tcttgttggg cgagcccgat ctgacgaccg cgcgctatcc
     3241 ggatcagctc gagatcggcg gcgtgcctct tcacttgagc tatcgcttcg agcccggcga
     3301 agacgacgac ggcatcacga tcaccgtgcc actcgcagtg ctgccgcagc tcgaccccga
     3361 cgtcatggag tggacgattc ccggctggca ccagccgaag atcctcgcgt tgctcgagag
     3421 tttgccgaag tccctgcgca agaaactgca gccgctggac ttgctcgcga ccgagctcgc
     3481 ggcggcggtg cagccgttct cggggcccat gctgccggcg ctcgcgcgcg cgatttatca
     3541 gaagaccggc gatcgcatca cgcccgatca gtgggacctg cgcgctgtgc cgcgctacct
     3601 cgatttctcg tatcgcgtca tcgacgatca cgacaagcca ctcgcggcga gcaaggatct
     3661 cagggacctc caagaccagc tcggcaatcg tgcgcgtgcg ttatggactg cgtctccgcg
     3721 cgaagcgatc gagagatccc agttgcgaac gttccccgat gcgttgccgg agactgtcac
     3781 ggtcgcgatc aacggccgca aggtccaagc cttcccggcg ctcgtcgatc gagagcatca
     3841 cgtcgacctg aagaccctcg agtcggcgcc ggcagcgttg caagcgaccc gcgagggcct
     3901 ccgccggctg tatctgttcg acagcggcaa ctcgttttcg cggctcgacg cacagatgca
     3961 tgcgccgctc ggcgatcgcc gggctttggt gatgcgtgcg ctcgacgagg ccttccatct
     4021 gaccgcgcca ccgccgcgca cgcgcgccga ctacgagcgc cggctcgagc tggggtgcaa
     4081 cgcgttgccg atcgaagtag cgcggtccgg acggatcgca tcggagctct cggccgagct
     4141 gacgccgatc aaggcggcgt tgcaaccact cgtcggcaag ccgggcgtcg cgaagatctt
     4201 cgtggacgac gtgcaaagtc agctcgcgca tctagcaccg ccagacttgt atcagcggac
     4261 cggatttgat cggctcgaag cgatcgtgcg ttacctgaag gcgctgcgca tccggatcca
     4321 gcgccaggct gtcgatccga tgaaggatca gcagaaggcg acgcacgtgg tgccgctatg
     4381 gcagcagtac atcgcgcagc ggccggcatt gctcgcgaag ggacgtaccg agaaggaact
     4441 agatgagttc agctggctcg tcgaagagct gcgcgtccag atcttcgcgc ccgaagtgaa
     4501 gccggcgctc tcgatcaacc tcgcgcgcgt gcaagacgcg tgggcgaagc ttcgctgatc
     4561 gcgactggct caggcgccga tctggaggcc gatcatcagc cgaaattgct cgtagtagag
     4621 tgggatcaga ccgaggttcg gggcgggcac cgcgatctcc accggatcga gcacgagtcg
     4681 cacggcgcct cccagatcgt aatcaatgcc aagcggcgtg aacgatacga atggacccat
     4741 gctgtacatc ggcgcgccgt tgacgtcgaa caagagcgtc gagacgccga cgtggacgga
     4801 cgtgcggagg ttgacgcgat tataggccat cggaaaccga cgaacgagcg tcgcgtacac
     4861 gctggcgaca ccgcgtttca tcgtgatcgg gacggccgtg aaccacgggt tccattcggc
     4921 atccaaaccg atcagccagt tctcgttcag gcggtagcga gcaccgatcg agatgtcggc
     4981 cgcagcatga acgatcgagc cgccgagcat cgcggacacg ccgatccgac ttcccagacg
     5041 cttgtcctcg cgaacctctg agagctcgcc gacggtcagg ctcgacgcgt ctcggcctgg
     5101 ctcggtgcct tcgcgcacgc tcgcggcatc gccaggctcc gtcgaggtga tcgggaccgc
     5161 gggttcactc gcagcggcgg gcggcgtagc ggtggcgggg tccgtgggtg ccgtcggcgc
     5221 ggtcggtgcc gtggtgtccg cgtgagccgg aagagccgca agcgtcagcg tgatcgcggc
     5281 aacgcccacg atgtggttgc gacggcggcg cgccaggaga acagcggcga tcacgaacaa
     5341 cgctgttgac cacgatcgac cttgtcgccc ggcatcacag cctacagccg caacgcttgg
     5401 ctctggcgcg tcgcagtagt tgttatcgaa cgtgcaccca ggttggtagc tgaagtactt
     5461 gatcgccaga gtctcgaacg tggcgatctt ctgctcgcgt gtcatcgcgg gatcgagcga
     5521 ggccgcgagg aaggcggtcg aggcttcaat cgcgagctgc tggttacgag cgacgagcgg
     5581 gtcggggctt tcgcagtgat ccatcggcgc gagatgcact gggccgtccg tgagctccgt
     5641 cgcggccgtt ccgctcgtgt tgtcgatcca gttcatgacc accgtgacga gcgcgccatc
     5701 gctcgttcga tacgtgtggg tgaaaccgtc ctcgactgcg tgcacggcct gtcccatgcg
     5761 tatgtagaac aagggcaact ccggcttcac gtggccggtg aacgatacgt agacgggaag
     5821 cggtacacgg atgtttggat cgacctcgcc gctggcggtc agcccgtcga gagcctcggt
     5881 tgcgcgccgc acgatgaacc tgcggcacgc cgcaagcgcg ctctcgttgc ccgcgtcacc
     5941 gtcgtcggtc tcggcgcgaa tgcagtgctc ctcctgcgtg atcggatcgc cgtggacgcc
     6001 gacgaggctg agcgtgttga gtggattatg gcctttgagg tcgctgtccc gaacgccgag
     6061 caggagcgct atcggcgcca ggtcatcaac aaaatcggat ggcggcacga actgcacctc
     6121 gccgatcaac cccgcttctt ctcgattggg cacgattcgc ggcgcggtca tgtacagcgc
     6181 gcgagcgttg cgaaatgcag tcgccgtgat tcgctcgtgg cagccctcgt agttgagctg
     6241 gctaccaatc gaccacgcgc ccgcttcctg gggcaccagc agtccgaccg tgatgaatga
     6301 gatgagagcg aaaacgcggt gaggtgacgt caagtcggaa ccgccattgc acgcgctgcg
     6361 ccacgctgga attcggcgag ttcagccact tgaagagcgg gctcgtgggc gaaaacgccc
     6421 aagcttgtca agcaagtccg gatcgcgtcc caattacgac cgcggcgttt tgcgggtctt
     6481 cccacggctc aaatcatccg cgccggaggg cgtcttttcg gtgcgatcag acacccgggc
     6541 tcgacgagat ggcttttccc gtaggtactt gggcctggag tgggctaaaa agctggaatc
     6601 cgctaccgag cgagggcccc tcgggggcgg gtgtaatgaa tgcctgggcc agtcttgaga
     6661 atcgatgaac actcgtctct acgttggtaa cctttccttc aacacgaccg ctgacggcgt
     6721 gcgcactgcc ttcgagcagt tcggcactgt gacggacgtc cacctcgtga cagatcgcga
     6781 aacgggccgc gcgcgcggct tcgcgttcgt caccatgagc acgccggatg aagcggcgaa
     6841 ggccatgcaa agcatggacg gccagaccct cgacggacgg ccgcttcgtg tgaacgaagc
     6901 cgagcagcgc cagcagcgcg gcggcgg
//
LOCUS       DATLBZ010000193        23349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2421545, whole genome shotgun sequence.
ACCESSION   DATLBZ010000193 DATLBZ010000000
VERSION     DATLBZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 23349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23349
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2421545"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VMZ53_10085"
     CDS             <1..411
                     /locus_tag="VMZ53_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28852.1"
                     /translation="AIDDHHTYYWQVVEGEVYPGHEFDDAGMFPGELTADDFHAAGVT
                     IVMTPIAMPDAILAEVATRATTLSEVLQDAWREAGDEVPAGFRAPSGPRHMHSIYLPV
                     DVWAELQDRAKTEERSVSYLVQRAVTAAYELPVE"
     gene            415..936
                     /locus_tag="VMZ53_10090"
     CDS             415..936
                     /locus_tag="VMZ53_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HUS28853.1"
                     /translation="MPYVMVDIEADGPIPGDYSMISLGAVVVEPALTRAYGIQLKPIS
                     DKWVPEALAVSGYTREQTLEFGDPATEMKRFGEWLKKLGSQPVFVSDNNGFDWMFTCW
                     YFHHFTGSCPFGFSSQNLGSLYKGLMKDMRASFKHLRKTKHTHDPVDDATGNAEAMLH
                     MIDQMGLRPSPGL"
     gene            complement(944..1336)
                     /locus_tag="VMZ53_10095"
     CDS             complement(944..1336)
                     /locus_tag="VMZ53_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28854.1"
                     /translation="MKPIAIAIAFSLSLLFFAACDSGATVCEGDHCVCAAGETCAHDC
                     DNGGTACHIQCEPGSSCDVGCASGEECHVECSASSSCNVDCNNGPDCNVTCPASGCTV
                     TNCPAGSCDVTCGLGSLATRSGSTASCN"
     gene            complement(1396..1677)
                     /locus_tag="VMZ53_10100"
     CDS             complement(1396..1677)
                     /locus_tag="VMZ53_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28855.1"
                     /translation="MSGGQNRDDLVAFAGFCAARIARDLRGIDAWDLFVVSGLDGHAD
                     AVVRVAVGAETVEARASACDPAHAIWNAMCQVEQPLRDAVIFAARRPAA"
     gene            complement(1913..3526)
                     /gene="murJ"
                     /locus_tag="VMZ53_10105"
     CDS             complement(1913..3526)
                     /gene="murJ"
                     /locus_tag="VMZ53_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HUS28856.1"
                     /translation="MPAEPASPPAAPQKGGRGGALAVAAGIFLSRIAGLVRERALSHY
                     LGLSPAADAYTAALRIPNFLQNLFGEGVLSASFIPVYSRLLAEGRTEDASRVARTVAT
                     LLALIASVIALLGVVAADALVSLIVPGWQGAKRELTVTLVRVLFPGVAILVLSAWCLG
                     VLNSHRRFFLSYVSPVLWNTAIIAAAVLGGRRLAGDVDDIALWVAWGAVIGSGAQLGV
                     QLPLTIKLLRGFKPSLAVRDEGVKQTVRAFGPVFIGRGSVQLSGYIDQFIASYLGNAI
                     VSALGKAQILYLLPVSLFGMAVSAAELPEMSSATGDEAARAEHLQKRLRAALRRVVFL
                     VVPSAVAFIAIGGFIVGLLFETGRFTAHDSDVVWIILAGSAIGLSAGTQGRLLSSAFY
                     ALGDTKRPLYAALVRVALTAVAGWAFALPLRETLGYSEMWGAFSLTASAGVAAWIEFL
                     LLRRWLERRIGRVPVPAQLGIGALAAAAVAGAAGYGAGQLALQLGAKHWAAAFVAIPI
                     FGAIYLGIMIVARVPEADPFARRLRLRRR"
     gene            complement(3581..4495)
                     /gene="gspC"
                     /locus_tag="VMZ53_10110"
     CDS             complement(3581..4495)
                     /gene="gspC"
                     /locus_tag="VMZ53_10110"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HUS28857.1"
                     /translation="MLGRALHHLVTAFFRRTWPLTVMTVFVCAAFAARGAASLLDASV
                     PEPSPPQVSAPHPVSAPVTAARAKDGNAFAERNMFCSSCAPLVAAKPGSTDSFVPDAV
                     LIATSLGDEPYATLLVRSNQAQGSWGVGDRVPGLGTIQRISFVTTEIVDDSGRHGVLS
                     LLESSAGGRGEAGAATPSPTAAGDTAADPYADRLRKIDDSTFEVDRSLVRELVGGSMS
                     KAGARIMPQTDKTGALDGLKLTGVKPGGLASRLGLQNADILQAVNNQKIESANTLLAM
                     YAQLDTMNNVEIAGTRRGKPLTITLRLR"
     gene            4669..6546
                     /locus_tag="VMZ53_10115"
     CDS             4669..6546
                     /locus_tag="VMZ53_10115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28858.1"
                     /translation="MTSGVPKQIGRYQILDRVAVGGMAELFKAQLQGQLGFEKLVAIK
                     KILPHLAGDQSFVEMFIDEARITAQLDHKHIVAVFELGTDADTPYIAMQYVDGLDVLA
                     LLRECARAQIRLPPDLAALIARDVLDALDYAHNALDVHGKQLKIVHRDISPGNVLLSW
                     RGDVKLTDFGIARAAERRHKTEAGTLKGKYGYMSPEQVSGADVDARSDLFSVGILLAE
                     MVMARRLFTSTNDLDILLMVRDARLDRLHKYASEFPVELRVLTVRALQRRPEDRWQTA
                     AQFRDALDEWIRRTTRVSSRDLATLIGRVINSPTADLERDQQTPQPETSGLSGPLTRM
                     SMVQAEEEARQARAEFIAGEGIPEGITGDPGSSGVIAVGDDDIMPESPREQASGPREV
                     GDIKEHPVLRVVYRLAKTKGTGMLALEGRAGVLKEAYFAEGQPQFVNSNVESERLGNF
                     LLEQKALTPQALQRAVSVMHHFGGRLADTLVGLDLLDPLEAYRLLAKQVAAKLMEAFS
                     WQKGRYTWTPRQQNPFKSRSLHLDAFRVIGAGAAQLSEALVDEWLLTHKQGFVVREPA
                     QDSELSQFGLGEALLRVYSLLDGRTRLGDLAARVRSPEARVNLLRLAYLLVQTDFARL
                     S"
     gene            complement(6561..8921)
                     /locus_tag="VMZ53_10120"
     CDS             complement(6561..8921)
                     /locus_tag="VMZ53_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUS28859.1"
                     /translation="MTNAGGDKRDPSASDERATSATGAASGSDHRTSTASAAHGSDDA
                     TRPASGSDDATSASGDDKHAMPATGAASASDDAASEPTASLDTSGGKRGLRGGKPAIG
                     RPHGSSRIARAVRWLAPSLVAGAAGALGAGVWEGATVGSALGKGAAAGFVVIVAWPIL
                     FALSAIVRGLWAAWDEKALGLVDNRAGERSGGGERGGGERGGGAPTLAAWLAVIWLSA
                     LVIAWALFQGTWMLANATAFKPLSLSFAEPVLGVTAFVCLVAVSRPLARGLAWVFGKL
                     DARWRLTVPRILIGFGVVTLVVGYVLWRFLVKRRIGPVDLSLLHAPVVGLFVLALVHA
                     MWGRLGRGARRIVGPVVAAGAVAAIVAAVVTWRTAPSTTLAIWGDRPLAGLAIDRLFN
                     LDRIRARISLAQFRPVAKPDGAHPDIILVTIDTVRADHTPPYGGSAEMPLLRELGVKG
                     AVFEWAFSPSNVTRRSIPSMLTGLAPNRIRGRVVGWALRLDPRHVMLQERMLAGGYDT
                     AGFVCCYGFYGDDFRTGWQRGFDKLEIEPTGMKLAKAAREWLAKRETTNPQKPLFLWM
                     HILEPHNWQQGTGVPTNDDERRKFYDRALTASDAMIVELLGALSNRRPERAPIVIITA
                     DHGEGLGEHGHPYHSTDLYNSQIHVPLVVAGPGIKPGRVSETVSLTDLTPTVLELAGF
                     EAPKGASIDGHSFADLAQGLRASKPDGGVAFAAMIKDRSNPGGVLAIVRGGWKMINNG
                     DSAELYEVHEDPNERQNLVSVKAGVYNELTLLMQKFVAAGDDSPFD"
     gene            9247..10467
                     /locus_tag="VMZ53_10125"
     CDS             9247..10467
                     /locus_tag="VMZ53_10125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS28860.1"
                     /translation="MAVLFLLGFSSGLPNLLTSQTLQAWMDSVGLDLGRTANLNAIGL
                     AYTLKFLWAPVLDRFRLPFLGRRRGWLLTLQLLLVGAIASMGTIDPVAAPYALFLVAL
                     GVAFLSASLDVVIDAYNADVLAPEERGAGVATYVMGYRVSFIASNTLALILADQMPWR
                     VVYAVMAGLMVLGVVGTLLASEPAEPRNAPRRFDEAIVMPFVEFWRKLGARRFALLVA
                     FAMLYRFGDYFAQSLIISFFRRGAGFSLTQIALVNKGLGVAGMAVGGLAAGTLVARYG
                     VRRLLVGFGVMAAITNLLYMWLALVGHDFAVFCVAVGVDNATTALGTAAILSVFMASC
                     SPAVSATQFAILTSLSSIGQRVFGAIFAADVVASVGWPGFFATTAAMALPGLVLASII
                     SRDERNFAPTAAAP"
     gene            10477..11367
                     /locus_tag="VMZ53_10130"
     CDS             10477..11367
                     /locus_tag="VMZ53_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28861.1"
                     /translation="MAAPKTWLAIPVVAVASAGAAAALGMRAGAPDWEVAAATGLLAA
                     ALAAAVRAFAGPSLAAAVAGVAAALLGTLTVLELPHLVLSRGAIASAAAMFAICELVR
                     PMPPEESPLPSIGAAVLAGVLDPSFVALVPITGWRYVRGPWPRPRGALALPIAGALAC
                     ILAAVAAVVPSTFLSDVWTAWTQRDGVVHAPLDVIARAGDALGPITAVAAVAGLGVCA
                     TRGRLAVVSIGAIVVGALAVDLALGSVGAAVPTIAALGAGVAIARLTAMVRWPAGQTF
                     VGAVAGFMMVLAPALALALR"
     gene            11384..13090
                     /locus_tag="VMZ53_10135"
     CDS             11384..13090
                     /locus_tag="VMZ53_10135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28862.1"
                     /translation="MDDGDDTQGDRTVDERPQSARTARDSRPAKPHVTAERYRLGERI
                     GRGGMGEVIAARDEQIGREVAIKRIRTDEPSERAIKRFLREARIQGRLEHPAIVPVHE
                     LGRDSDGMPFFAMKRLVGKPLSKIIDDPERDRAKYGRQRLLRAFVDVCLATELAHVTG
                     IIHRDLKPDNIMLGDFGEVYVLDWGVAKIAGEDDDFDDIKNDNLQTSTGVAIGTPGYM
                     SPEQAMGEHIDARTDVYALGITLGEILDADPEPPPELVALRDRATAENREARIQTARS
                     LADEMQRFLDGDRDLELRRKLARDHLEIARAARTATDTVEARRTSMMQAGRALALDPE
                     LAEAGQLITQLLVEPPTVAPPEVIAQLEADKRDHARAIYRASMWSSLAYLTIVPGLVE
                     TGSLTYWIIAIVAAGTGFYTSLSGTRNPGSMLLTLGANALFAVVLARLYTPVFVAPGV
                     AIALAAGGSGDPRIAGRLGSLKLFVLTSAVVLVPLAAEQLGWLQPTMTMNPDGSLLLH
                     GLAMKGHIPLTPIIVVAVLSVIGIAVLVGHIERESAWAMRRAFHVQAWQVRQLMPDMR
                     RK"
     gene            13087..14028
                     /locus_tag="VMZ53_10140"
     CDS             13087..14028
                     /locus_tag="VMZ53_10140"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HUS28863.1"
                     /translation="MSILGAMQVIRGHERAPAWREGTAVAIGNFDGVHLGHRALIARA
                     RALADAHGAQVVALTFDPHPQALFAASPPPMISSLARRIELLGEAGADAVVVEPFTRA
                     LASKAPYAFIDDIVIQALGARAIVVGYDFSYGQGRTGTTDALRSHGGRAGVEVAIVSA
                     VDSGGEVCSSTRIREHLRAGELKAAERLLGRRWDVDGVVVHGAKRGRAIGVPTANIKP
                     ESDLPLRPGIYAVTLSVDGGPQHPAVASLGTNPTFVEHGGSLVLEVHVLDFDEDIYGK
                     RTRTTFVQHLRDEAKFDSVDALVVQIRADIDAARRVL"
     gene            14142..16943
                     /locus_tag="VMZ53_10145"
     CDS             14142..16943
                     /locus_tag="VMZ53_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS28864.1"
                     /translation="MRFRTLAIATALYAGLTQTGAAYVAQKGAERPTVAAGRPARLHR
                     DVAWAAPQGALAGLPSWRVIWDRDTDVPLRMWGPPISAPNANRDAAAAETAARAFLAQ
                     HGSMLAPGANAGDFTMLVNRVDGALRTVTFAQHANGLRVVGGSLAFTFSHDRLIMVSS
                     TALPNVSVRMPGGSLPFATLASTARDWLGQAGHSVDVKAQGDRVIIPIVHARGNRGTP
                     QITYKVAETLTVESTREEAGRWDVWLDAANAAPIARHQTLMFASAKILFDTGDRWPMG
                     TRSPKAAPEVNHTVVSSATAVTSLMDGTITWAGDAAAQVTIGLVGPHIKITNKQGALA
                     TDTLTLQPDQTYVWSKASEEFGDAQLTSFVAASQAKAFVRTRINPNLAWLNNQLPVSV
                     NENQTCNAFSTGNDIHFYKGGSQCENTGRIADVVYHEFGHSVHAQSIIDGEGSFDSSL
                     SEGLADTLAAAITGDHGMGRGFFHNDSPLRDLDPVGKELKWPDDADGEPHDEGEIIGG
                     TLWDLRTALEAQYGQEAGFNKFLVLYYGIVQRASDIPSSYAAALTVDDDDGDLSNGTP
                     NQCLIDNAFGKHGLSDPTVTLGLTAPVREGFRISFELHPPTQTACPPPGVSKATLTWA
                     PRGGTAAEVAVTESGGLWSANIPTQPDGTVVNYHVTVQLEDGSSISYPQNPADPDYQF
                     YVGNVEEIWCANFEDGIGEWTVGGTPAQRNEWEAGAPAGVGGDPKAAYEGNGVLGIDI
                     GDQDDGLYNPRTTQFVESPVIDLGGETNVRLQYYRWLNAEDAAYDKATIFANDEQVWQ
                     NYATPGTMPDTEVNHTDKEWRFQDVDLTAQAAAGSLKLKFQLISDAGLEMSGWTVDHV
                     CIVKAGPPSNLCGNHNVDEGETCDDGNTADGDGCSATCGGDEAGDGGCCSAGTNPAGP
                     ALLSLVTLGLVVLRRRRRR"
     gene            16982..18958
                     /locus_tag="VMZ53_10150"
     CDS             16982..18958
                     /locus_tag="VMZ53_10150"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS28865.1"
                     /translation="MRHVLIALAFSAACTRADKEPLYKGEMPIAFGECAGPSVAFVSG
                     PRPLPFEAATVENLVAGKVAKDEVPKPEPEDPPPNENGGGFASLIGTGDISSGFDDTN
                     IYGGLLGNEAGEMNGGFGFGRSGFGPGGGGQGIGTIGTGRYGTIGHGSGTGSGYGVGG
                     GRGGMRGRTSAVPTVSIGQPNAQGDLDKAIIRRYIKRNIQKITYCYEKQLLVKPKLAG
                     TVQTQFFITPNGNVAKSSGSGVDPEVADCVAGVIKDIEFPKPKGGGGVQVNYPFTFRP
                     AGDTTTIADAPAAGAGSDAGSAAAPPAPDAGSAAAAPAPTGELRHVFRAWGTPVPDAS
                     KYDPGANNPLRALEKDLSQCFRRGPNHFGAVVVELTYDASGKTTNAVPHGVTHESARK
                     CVEKVAKRVTRGAGAATTQRCSLSFGVMPVSALPAIDITSKGIALDGRSLITIADADT
                     QSPGLARTIPSVREAIEARVDAVTQTDRVVTAHGPLVIKPLPKTPMKYVDGVARSVLR
                     GGDDFVLATSDGSEWKLLSPLTLPLVPVPFSSGGRWNRYKGRFLDGTVSQDDERVVLS
                     ILISSDRMWLGLSRVNEFQELPAGPDQLGKLAAALKAQKASAFFVDRDDIEIAGDSDV
                     PYETFVKIVEAAEAAGFKTWQHTSPDGLAARPQL"
     gene            19060..19731
                     /locus_tag="VMZ53_10155"
     CDS             19060..19731
                     /locus_tag="VMZ53_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28866.1"
                     /translation="MTLVRRSAGFLVALALVTACEKKRESHASLAQKERERQARLVIP
                     DLTEPEHAPPPTAPPAPEAPSPDAIRKLHAEPIAGVNLGMTDAQVTKILGAPTNKSAI
                     AALGNTKNEFGMTWTWPTMKAELAGATKKGPFTLRSITFLRASKVKTSRGVGIGSTRA
                     EIASAYAGLEDPIFAKDTRMFVVGASTSYGMNGISFNFRDDDRDGNLETDRVKEIEWA
                     GGAEQ"
     gene            complement(19750..20712)
                     /locus_tag="VMZ53_10160"
     CDS             complement(19750..20712)
                     /locus_tag="VMZ53_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HUS28867.1"
                     /translation="MRLVVVCSLVGVFATACAVGEPSEDELAVIEAQLANGRYSTQRF
                     HLPPRTAVYDANGHWVATFTDGARTVRVKGPSRTLHERTAIDIRNTHWVRVLDTPFGG
                     TVDVAWLDAARVDTSNDVIARALQYAEGAPPITSNTTGLQIAGDASYGPLVDGARQEG
                     SDFNDYLGIPWTYSDGTRDTNEAAQLHSLDCSGFMRMVWGYRHHVPLTAGADGDGRAM
                     PRRAVQILASGPGVVTIDVDAHASEDVDRLFVGDLVFFDASTDDGTAIDHVGMYLGID
                     VDGHPRFISSRKSINGPTFGDFSGASTLDGTGLYARSFRAARRL"
     gene            complement(20863..>23349)
                     /locus_tag="VMZ53_10165"
     CDS             complement(20863..>23349)
                     /locus_tag="VMZ53_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28868.1"
                     /translation="ALLPRHPDLLVRIFPVLGAVPAIAAAPTARTPVTDPHEQRNQAF
                     AALRDVLHRLSSRRRLVLTIDDWQWADPDSVVLARDLVRHRDAPPILLVLTARPSDDA
                     ETAARIEAVKTAEVRRISLESLALAPAIELAGQLRAAFAPQLQLDLDAIARETGGHPL
                     YISELVRYAATKGSADAAATVRLEEAILARIAGLPPEARAIVDVLAVAGEPITLEVVR
                     DALDEKMSVVQRQSASLRIAHLVRSGHADGSLEPYHDRVRQAVLDAMPPAERSAWHKK
                     LASALEASPLARARPELLIWHLEAAGESTRAAELAGEAAQRAVSAGAFDQAASLFALA
                     LRGSHVDAQRARALRTEMGQALANAGRGAEAAEAFLDVARDADPATRLSCHRQAAEQL
                     LSVGEIDRGLEILRALLADVGVKMPKTQKSALASVVWTRAKLRMRGLRWKERRESQIA
                     PETLLRLDVLKAASQSLALVDNIRAAAFNARWLLLALQIGENIRTALAVLTETVYQSS
                     QGGRGVVRSRALLDSVRKVCQESNDPRLRAFLLMSEGTVEYWTCRLARADELIGQAEA
                     VFREQTTGTNLELKSARMFHAFALRHRGAWARLRDLRERYVADAERRGDRYVITSMNR
                     YCSALYLAADDTRRARNMVDNATWVLPTTAFHAQHWYELEARGEIALYEGSVARDMPT
                     LEPLFDGLAKSVLTRVTTVSALSLWLRGRLALCTEGSDAQKLVERAIANLPKVDNPRV
                     DVFGALLRAGLAMRAGNDDVAIAQLRAAYTRADELDLSLHAAAARRQLGVLLGASEGA
                     AHIAAAEKTMRDEGVASPERFTRWFVPGSR"
BASE COUNT         3918 a         7800 c         7873 g         3758 t
ORIGIN      
        1 gcgatcgacg atcaccacac gtattactgg caggtcgtcg aaggcgaggt ctaccccggc
       61 cacgagttcg acgacgccgg catgttcccc ggcgagctga cggccgacga cttccacgcc
      121 gcgggcgtca cgatcgtgat gacgccgatc gcgatgccgg atgcgattct cgccgaagtg
      181 gcgacgcgcg cgacgacact ctcggaggtc ttgcaggacg cgtggcgcga agcgggcgat
      241 gaagttcccg ccggcttccg cgcgccgagc ggcccgcgtc acatgcactc gatctacttg
      301 ccggtcgacg tgtgggccga gctccaggat cgcgcgaaga ccgaggagcg cagcgtctct
      361 tatctcgtgc agcgcgcggt gaccgcggcg tacgagctgc ccgtcgagta gcccatgccg
      421 tacgtgatgg tcgatatcga ggcagacggt cccatcccgg gcgactactc gatgatctcg
      481 ctcggtgcag tcgtcgtcga gccggcgctc acgcgcgcgt atggcatcca gctaaagccg
      541 atcagcgaca agtgggtgcc cgaagcgctc gcggtcagcg gctacacgcg cgagcagacg
      601 ctcgagttcg gcgatccggc gaccgagatg aagcggttcg gcgagtggct gaagaagctc
      661 ggctcgcagc ccgtcttcgt gtccgacaac aacggcttcg actggatgtt cacctgctgg
      721 tacttccacc acttcacggg aagctgtccg ttcggattct cgtcgcagaa tctcggctcg
      781 ctctacaagg gcctgatgaa ggacatgcgg gcgtcgttca agcatctgcg caagacgaag
      841 cacacgcatg atccagtcga cgacgcgacg ggcaacgccg aagcgatgtt gcacatgatc
      901 gatcagatgg gccttcgtcc gtctcccggt ttgtagcgcc gattcagttg cacgacgcgg
      961 tcgacccact gcgcgtcgcg agcgaaccga ggccgcacgt gacatcacaa ctgcctgctg
     1021 ggcagttcgt caccgtgcag ccagatgcgg ggcacgtgac gttgcagtcg gggccgttgt
     1081 tgcagtcgac gttgcagctc gacgatgcgc tgcactcgac gtgacattcc tcgccggagg
     1141 cgcagccgac gtcacacgac gagccgggct cgcactggat gtgacacgcg gttccgccgt
     1201 tgtcgcagtc gtgcgcgcac gtctcgccag cggcgcagac gcagtggtcg ccttcgcaga
     1261 cggtggcgcc ggagtcacag gcggcgaaga agagaagcga gagcgagaat gcgatggcga
     1321 tggcgatggg cttcatatgc ccatgattcg ccgaagcggg gcgaatcgcc gatatcagcc
     1381 cgacctcagc ggctgttacg cggcagggcg gcgtgcggcg aagatcacgg catcgcgcag
     1441 cggctgctct acctgacaca tcgcgttcca gatcgcatgc gcgggatcgc aggcgctcgc
     1501 gcgcgcttcg acggtctcgg cgccgacggc gacgcgaacg acggcatccg cgtgaccatc
     1561 gagtccggac acgacgaaca ggtcccacgc gtcgatgccg cgcaggtcgc gcgcgatacg
     1621 agccgcgcag aatccggcga acgcgacgag gtcgtcacgg ttctgaccac cactcacggg
     1681 tggagcgagc ctcagctccg cacgcacccg cacctcgcac acttggcttg ccattaacgc
     1741 cgcgcaagag cacgaacagt gccgcgcgcg agccgagatc tctccgatcg taagtgatcg
     1801 aaatgatccg aatgcacggt tcgcgcaggg caggcgactc gagtagccag tgtgcagatt
     1861 cgcccgctga tccactttca gcgttgcgat cgcaattcac cgcgatcggg gttcagcgac
     1921 gacgaaggcg aaggcgacga gcgaacgggt ccgcttcagg cacgcgcgcg acgatcatga
     1981 tgccgagata gatcgcgccg aagatcggaa tcgcgacgaa cgcggcggcc caatgtttcg
     2041 cgccgagctg cagcgcgagc tgtcccgcgc cgtagcccgc agcgcctgcg accgcggcgg
     2101 ccgcgagtgc accgatgccg agctgcgcgg gaaccggcac gcgaccgatg cgtcgttcga
     2161 gccagcggcg cagcagcagg aactcgatcc acgccgcgac gcctgcactc gcggtgagcg
     2221 agaacgcgcc ccacatctcg ctgtaaccga gcgtctcgcg aagcggtagg gcgaacgccc
     2281 aaccggcgac ggcggtgagc gcgacgcgca cgagcgctgc gtagagcggc cgcttcgtat
     2341 cgccgagtgc gtagaacgcc gagctgagga ggcggccttg ggtgcctgcc gagagcccga
     2401 tcgcggagcc ggcgaggatg atccagacga cgtcgctgtc gtgcgccgtg aagcgtccgg
     2461 tctcgaacaa caagccgacg atgaagccgc cgatcgcgat gaacgcgacc gccgagggca
     2521 cgacgagaaa gacgacgcgg cgcaacgcgg cccgcagtcg cttctgcaaa tgctcggcgc
     2581 gcgcagcttc gtcgcccgtc gcgctcgaca tctcgggaag ctcggcggcg gacaccgcca
     2641 tgccgaagag gctgaccggc agcagataga ggatctgcgc cttgccgagt gcggagacga
     2701 tcgcgttgcc gaggtagctg gcgatgaact gatcgatgta gccggagagc tgaaccgagc
     2761 cgcgaccgat gaacacgggg ccgaatgcgc gcaccgtctg cttcacaccc tcgtcgcgga
     2821 cggcgagcga cggcttgaac ccgcgcagga gcttgatggt caacggcagc tgaacgccga
     2881 gctgcgcacc ggagccaatg accgcacccc acgcgaccca cagcgcgatg tcgtcgacgt
     2941 cgccggcgag gcgtcgaccg ccgaggacgg ctgcggcgat gatcgccgtg ttccacagca
     3001 cgggtgacac atacgagagg aagaaccggc ggtgactgtt gagcacgccg aggcaccacg
     3061 cactgagcac gaggatcgcg acgccgggga acagcacgcg aacgagggtg acggtgagct
     3121 cgcgcttggc gccctgccag ccaggcacga tgaggctgac gagcgcgtcc gcagcgacga
     3181 caccgaggag cgcgatcacg ctggcgatga gcgcgagcag cgtcgcgacg gtgcgcgcga
     3241 ctcgcgatgc atcttcggtg cggccctccg cgagcagtcg cgagtagacg ggaatgaaag
     3301 atgccgagag gacgccctcg ccgaacaggt tctgcagaaa gttcggaatg cgaagcgctg
     3361 ccgtataggc atcggccgcg ggcgagaggc cgaggtagtg cgagagcgcg cgctcgcgaa
     3421 cgaggccggc gatgcgcgag aggaagatgc cggcggcaac cgcaagcgcg ccgcctcgac
     3481 cgcccttttg cggcgcagcc ggtggtgacg caggctctgc aggcatcgac gcgattctcg
     3541 tctaacgccc gagatcgcgc ccactattcg cgcgatgaat tcagcgtagc cgcagcgtga
     3601 tcgtgagcgg tttgccgcga cgcgtgccgg cgatctcgac gttgttcatc gtgtcgagct
     3661 gcgcgtacat cgcgagcagc gtgttcgcag actcgatctt ctggttgttc acggcttgga
     3721 ggatgtcggc gttctgaagg ccgaggcgac tcgcgagccc gcccggtttg acgcccgtga
     3781 gcttgaggcc gtcgagcgcg ccggtcttgt ccgtctgcgg catgatgcgc gcgcccgctt
     3841 ttgacatcga gccaccgacg agctcgcgga cgagtgaccg atcgacttca aacgtcgagt
     3901 cgtcgatctt gcggagacga tccgcgtacg gatccgcagc ggtgtcgcca gcggccgtgg
     3961 ggcttggcgt tgccgcgcct gcctcgccac ggccgccggc cgagctttcg agaagtgaga
     4021 ggacgccgtg acgtccgctg tcgtcgacga tctcggtcgt cacgaacgag atgcgctgga
     4081 tggtgccgag accgggcacg cgatcgccga cgccccagct gccttgcgct tgattggagc
     4141 gcacgagcag cgtcgcgtac ggctcgtcac cgagagacgt cgcgatgagc accgcgtcgg
     4201 gaacaaacga atccgtcgaa cctggcttcg cggcaacgag cggtgcgcac gacgagcaga
     4261 acatgttccg ctcggcgaat gcgttgccgt cttttgcgcg agctgcggtc acgggagccg
     4321 acacaggatg aggagctgag acctgtggag gggacggctc cgggacgctg gcatcgagca
     4381 acgaggcggc accgcgtgct gcgaatgcag cgcacacgaa gacggtcatc accgtgagag
     4441 gccaggtccg acggaagaag gcagtgacta gatgatgaag cgcacgtcct agcatcactg
     4501 acctcgtgca gaggcttcat caacacgcgt gccgggcgcg ggacctgcgc aaggtcgcga
     4561 agtgaggtag cgttccgcct gggtcgcgcc acgagggcgt tcccggcgcc gaacacgctg
     4621 ccaatgtggc agggcgctta aagaagcggc tgttatgatg aggatccagt gacaagcggc
     4681 gtacccaagc agatcgggag atatcaaatc ctcgatcgcg tggccgtcgg cggcatggcc
     4741 gagctgttca aggcgcagct ccagggccag ctcggcttcg agaagctggt ggccatcaag
     4801 aagatcctgc cgcacctcgc gggcgatcag agctttgtcg agatgttcat cgacgaggcg
     4861 cggatcaccg cgcagctcga tcacaagcac atcgttgcgg tgttcgagct cggcaccgat
     4921 gccgatacgc cgtacatcgc gatgcagtac gtcgatggcc tcgacgtgct cgcgctgctg
     4981 cgtgaatgcg cgcgcgcgca gattcgtctg ccgcccgatc tcgcggcgct gatcgctcgc
     5041 gacgtgctcg acgcgctcga ctacgcgcac aatgcgctcg acgtccacgg caagcagctc
     5101 aagatcgtcc accgcgatat ctcgccgggc aacgtgctgc tgtcgtggcg cggcgatgtg
     5161 aagctgacgg acttcggcat cgcgcgcgcg gcggagcgcc gccacaagac ggaagccggc
     5221 acgctcaagg gcaagtacgg ctatatgtcg ccggagcaag tgagcggcgc ggacgtcgat
     5281 gcgcgcagcg atctgttcag cgtcggcatc ttgctcgccg agatggtgat ggcgcgccgg
     5341 ctgttcacgt cgacgaacga cctcgacatt ctgctgatgg tccgcgacgc gcggctcgat
     5401 cggctgcaca agtacgcatc cgagtttccc gtcgagcttc gcgtgctcac ggtgcgcgcg
     5461 ctgcagcggc ggcccgagga tcgctggcag acggcagcgc agttccgcga tgccctcgac
     5521 gagtggattc gccgcacgac gcgtgtgagc tcgcgcgacc tcgcgacgct catcggccgc
     5581 gtgatcaact cgccgacggc ggacctcgag cgcgatcagc agacgccgca accggagacg
     5641 tcggggctct ccggcccgct cacgcgcatg agcatggtgc aggccgagga agaagctcgc
     5701 caagcgcgcg ccgagttcat cgcaggcgag ggcatccccg aaggcatcac aggcgaccct
     5761 ggctcgagcg gcgtgattgc cgtcggtgac gacgacatca tgccggagtc gccgcgcgag
     5821 caagcctcgg gtccgcgcga ggtcggtgac atcaaggagc atcccgtcct gcgcgtcgtc
     5881 taccggctcg cgaagacgaa gggcactggc atgctcgcgc tcgaaggtcg cgctggcgtg
     5941 ctcaaagagg cgtacttcgc tgaaggccag ccgcagttcg tgaattcgaa cgtcgagagc
     6001 gagcgcctcg gcaacttcct cctcgagcag aaggcgctga cgccgcaagc tttgcagcgc
     6061 gccgtgtcgg tgatgcacca tttcggtggc cggctcgccg acacgctcgt cggactcgat
     6121 ctgctcgatc cactcgaggc gtatcgcctg ctcgcgaagc aagtcgcggc gaagctgatg
     6181 gaagcgttct cctggcagaa gggccgctac acgtggacgc cgcgccagca gaatccgttc
     6241 aagtcgcgat cgctccacct cgatgccttt cgcgtgatcg gcgccggcgc agcgcagctc
     6301 tccgaggcgc tcgtcgacga atggctgctg acgcacaagc aaggcttcgt cgtgcgcgag
     6361 ccggcacaag actccgagct gtcgcaattc ggtctcggcg aggcgctgct gcgcgtctac
     6421 agcttgctcg atggtcgcac gcgtctgggc gatctcgctg ctcgcgtgcg ctcgcccgaa
     6481 gcgcgggtca acctgctccg cctggcgtac ctgctcgtgc agaccgactt cgcgcgactg
     6541 tcgtagtcgc ctggccgccg ctagtcgaac ggcgagtcgt cgcccgctgc gacgaacttc
     6601 tgcatgagca gcgtgagctc gttgtagacg ccggccttca cgctgacgag attctgccgc
     6661 tcgttcggat cctcgtgcac ctcatacagc tcggcggagt cgccgttatt gatcatcttc
     6721 cagccgccgc gcacgatcgc gagcacgccg cctggattgc tgcggtcttt gatcatcgcc
     6781 gcaaatgcga ccccgccgtc gggcttgctc gcgcgtaggc cctgcgcgag atccgcgaac
     6841 gagtggccgt cgatgctcgc gcccttcggc gcttcgaagc cggcgagctc gagcactgtc
     6901 ggcgtgagat ccgtgaggct gaccgtctcg ctcacgcggc cgggcttgat cccgggcccc
     6961 gcgacgacga gcggcacgtg aatctgcgag ttgtacagat ccgtcgagtg atacgggtga
     7021 ccgtgctcgc cgaggccctc gccgtggtcg gcagtgatga tcacgatcgg cgcgcgctcg
     7081 ggcctgcgat tggacagcgc accgagcagc tcgacgatca tcgcatccga cgcggtgagc
     7141 gcacggtcgt agaacttgcg gcgctcgtcg tcgttcgtcg gcacgccggt gccttgctgc
     7201 cagttgtgcg gctccagaat atgcatccac agaaacagcg gcttctgcgg gttggtcgtc
     7261 tcgcgtttcg ccagccactc gcgcgcggcc ttcgcgagct tcatgccggt cggctcgatc
     7321 tcgagcttgt cgaagccgcg ctgccagccg gtccgaaagt cgtcgccata aaacccataa
     7381 cagcagacga agccggccgt gtcgtaaccg ccggcgagca tgcgctcctg cagcatcacg
     7441 tgccgcggat cgaggcgcag cgcccatccg acgacgcgcc cgcggatgcg atttggcgcc
     7501 aatcccgtca acatcgacgg aatcgatcgc cgcgtcacgt tgctcggcga gaacgcccac
     7561 tcgaacaccg cccccttcac gccgagctct cgcagcagcg gcatctcggc actcccaccg
     7621 tacggcggcg tgtgatccgc gcgcaccgta tcgatcgtca cgaggatgat gtcgggatgg
     7681 gcgccgtcgg gcttcgcgac gggccggaac tgcgcgagcg aaatgcgcgc gcgaatgcgg
     7741 tcgaggttga agagccgatc gatcgccaat cccgcaagcg gccggtcgcc ccagatcgcg
     7801 agcgtcgtgc tcggcgccgt tctccacgtc acgacggccg cgacaatcgc cgcgaccgcg
     7861 cccgccgcga ccaccggccc gacgatgcgc cgcgcgccgc ggccgagccg cccccacatc
     7921 gcatggacga gcgcgagcac aaacaacccg accaccggcg cgtgcagcag cgacaaatca
     7981 acaggcccga tgcgccgctt cacgagaaac cgccacagca catagccgac aaccagtgtc
     8041 accacgccaa agccgatcaa gatgcgcggc acggtcagcc gccaccgcgc gtcgagcttt
     8101 ccaaacaccc acgcgagccc gcgcgccaac ggccgcgata ccgcaacgag acacacgaac
     8161 gccgtcacgc cgagcaccgg ctcggcaaac gacaacgaca gcggcttgaa tgccgtcgcg
     8221 ttcgcgagca tccacgtgcc ttgaaagagc gcccacgcaa tcacgagcgc cgacagccaa
     8281 atgacggcga gccacgccgc caacgtcggc gcgccaccac cgcgctcgcc gccgccgcgc
     8341 tcgccgccgc cgctgcgctc gcccgcgcga ttgtcgacga gcccgagcgc tttctcgtcc
     8401 cacgcggccc acaacccgcg gacgatcgcg ctgagcgcga acaggatcgg ccacgcgacg
     8461 atcacgacga agccggccgc ggcccccttg ccgagcgcgc tgccaaccgt ggcgccttcc
     8521 cacacgccag cgcccagtgc cccggcagcg ccggcaacaa ggctcggcgc cagccaccgc
     8581 accgcgcgcg cgatgcggct cgacccatgc ggacgaccga tcgctggctt gccgccacga
     8641 agaccccgtt taccgccgct cgtatcgagg ctcgcggtcg gctcgcttgc cgcgtcgtcg
     8701 ctggcactcg ccgcgcccgt cgcaggcatc gcgtgcttgt cgtcaccgct agcgctcgtc
     8761 gcgtcgtcgc tgccactcgc cgggcgcgtc gcgtcgtcgc tgccgtgcgc cgcactcgcc
     8821 gtgctcgtgc ggtggtcgct gccgctcgcc gcgccggtcg cgctcgtcgc gcgctcgtcg
     8881 gatgcgctcg gatcacgctt gtcgccgcct gcgttcgtca cgatgtcggc gcctcttcac
     8941 cggccgcgtc ggctacgcgc gaacgctctg ccggaaccgc gcctttaacc gacctcttct
     9001 tgcgtccctt gctctgcttg ctcccttgcg ttccatctgg ggctgtcggg gctgtcgggg
     9061 ctgtcgcgcg tgtcgaggtc ttctcgccgc ttcttggcgc cgcggtctcg gcattaacgc
     9121 ccgccgagtc ggctttgttc gaggacgcca cgcgcgcctc gaattctacc tgcggggcgg
     9181 gtgcgtggca cgatcccagc tctgcaagcg cgttcgggtc ctttggcggt ttttgcgtcg
     9241 cggcggatgg ccgtgctgtt cttgctcgga ttctcgagcg ggctgccgaa tcttctgacg
     9301 agccagacgc tgcaggcgtg gatggactcc gttgggctgg acttggggcg caccgcgaac
     9361 ctcaacgcga tcgggctcgc gtacacgctg aagtttctgt gggcgcccgt gttggatcga
     9421 tttcggctgc cattcttggg gcggcgccgc ggctggctgc tgacgttgca gctcttgctt
     9481 gtgggggcga ttgcctcgat ggggacgatc gatccggtag cggcgccgta tgcgctgttc
     9541 ttggtggcgc tgggtgtcgc gttcttgtcg gcgtcgctgg atgtcgtcat cgatgcgtac
     9601 aacgcggatg tgctggcgcc cgaggagcgc ggggcgggcg tggcgaccta tgtgatgggc
     9661 tatcgggtgt cgtttatcgc cagcaatacg ctcgcgctga tcctcgccga ccagatgccg
     9721 tggcgcgttg tctatgcggt gatggcgggg ctcatggtgc tcggcgtggt gggcacgctg
     9781 ctcgcgtccg agccggcgga gccgcggaat gcgccgcgga ggttcgacga agcgattgtg
     9841 atgccgttcg tcgagttctg gcggaagttg ggcgcgcggc ggttcgcgct gctcgtcgcg
     9901 ttcgcgatgc tgtatcggtt cggcgattac ttcgcgcagt cgctgatcat ctcgttcttt
     9961 cggcgtgggg ctggcttttc gctcacgcag atcgcgctcg tgaacaaggg gctcggcgtg
    10021 gccggaatgg ccgtcggtgg gctcgcggcc ggcacgctcg tcgcgcggta cggcgtgagg
    10081 cggctgctcg tcgggtttgg cgtgatggcg gccatcacga acctcctcta tatgtggctc
    10141 gccttggtcg gccacgactt cgcggtgttt tgcgttgcgg tgggcgtcga caacgcgacg
    10201 accgcacttg gcacagcggc gatcctgtcc gtgttcatgg cgtcgtgctc gccggccgtg
    10261 agcgcgaccc aatttgcgat tttgacgtcg ttgtcgtcga tcgggcagcg cgtgttcggc
    10321 gcgatcttcg cggcggacgt cgtggcgtcg gtcgggtggc ctggattttt cgcgacgacc
    10381 gcggccatgg ccctgccggg cctcgtgttg gcgtcgatca tcagccgcga cgagcgaaat
    10441 tttgccccga cagcagcggc cccgtagcgt cgatccgtgg cagcacccaa gacgtggctc
    10501 gcgatccccg tggtcgcggt ggcctccgcg ggtgcggctg cggcgctcgg catgcgcgcg
    10561 ggtgcgccgg attgggaagt ggccgcggcc accgggttac tggcggctgc actcgctgcg
    10621 gccgtgcgcg cattcgccgg accgtcgctg gctgccgccg tggcgggcgt cgccgcagcg
    10681 ctgctcggca cgttgaccgt cctcgagctg ccgcatctcg tgctgtcgcg cggcgcgatc
    10741 gcgtccgccg cagcgatgtt cgcgatctgc gagctcgtgc gaccgatgcc gcccgaagaa
    10801 tcgccgctac cgtcgattgg cgcggcggtg ctcgctggcg tgctcgatcc gagctttgtc
    10861 gcgctcgtgc cgatcaccgg ttggcgctac gtgcgcgggc cgtggccgcg tccacgcgga
    10921 gcgctcgcgc tgccgatcgc gggtgcgctt gcctgcattc ttgccgcggt cgcggccgtc
    10981 gttccgtcga cgtttctgag cgatgtgtgg actgcgtgga cgcaacgcga tggcgtggtg
    11041 cacgccccgc tcgacgtgat cgcacgtgcg ggcgacgcgc tggggcccat caccgccgtt
    11101 gctgctgtcg ccggcctcgg cgtatgcgcg acgcgcggcc gcctcgccgt cgtcagcatc
    11161 ggcgcgatcg tcgtcggtgc gctcgcggtc gatctcgcac tcggcagtgt cggtgccgcg
    11221 gtccccacga tcgctgcgct cggcgcaggt gtcgcgatcg ctcgcctcac cgcgatggtt
    11281 cgctggccgg cggggcagac attcgtcggc gctgtcgctg gcttcatgat ggtcctcgct
    11341 ccggcgctcg ccctcgcact tcgctgagcg gtacgctcgg cggatggacg acggggacga
    11401 cacccagggc gaccgcacgg tcgatgaacg tccgcagagc gcacgcacag cgcgtgactc
    11461 gcggcccgcg aagccgcacg taaccgcgga gcgctatcgc cttggcgaac gcatcgggcg
    11521 aggaggaatg ggcgaagtca tcgctgcgcg cgacgagcag atcggccgcg aggtcgcgat
    11581 caagcgcatt cggactgacg agccgagcga gcgcgcgatc aaacgctttc tccgcgaagc
    11641 gcgcattcaa ggccggctcg agcatccggc gatcgtccct gtacacgagc tcggtcgcga
    11701 cagcgacggc atgccgttct tcgccatgaa gaggctcgtc ggcaaaccat tgtcgaagat
    11761 catcgatgat cccgagcgcg atcgcgcgaa gtacggccgc cagcggttac tgcgtgcgtt
    11821 cgtcgacgtc tgcctcgcca ccgagctcgc acacgtcacg ggcatcattc atcgcgatct
    11881 caagcccgac aacatcatgc tcggcgactt cggcgaggta tacgtgctcg attggggcgt
    11941 cgcgaagatc gccggcgagg acgacgactt cgacgacatc aagaacgata acttgcagac
    12001 gagcaccggc gtcgccatcg gcacaccggg ctacatgtct cccgaacaag ccatgggcga
    12061 gcacatcgac gcgcgcaccg atgtctacgc gctcggcatc acactcggcg agatcctcga
    12121 cgccgatcca gagcccccgc cggagctcgt tgcgctgcgc gaccgcgcga ccgccgagaa
    12181 ccgcgaagcc cgcatccaga ctgcgcgcag cctcgccgac gagatgcagc gcttcctcga
    12241 tggcgatcgc gatctagagc ttcgccgcaa gctcgcccgt gatcacctcg agatcgcgcg
    12301 tgctgccagg accgccacag acaccgtcga ggcgcgtcgc acgtcgatga tgcaagcggg
    12361 ccgcgcgctc gccctcgatc ccgagctcgc cgaagcaggc cagctcatca cgcagctcct
    12421 cgtcgagccg cctacagtcg cgccgcccga ggtgattgcg cagctcgaag cggacaagcg
    12481 cgaccacgcg cgcgccatct atcgcgcatc gatgtggtct tcgcttgcgt acctcacgat
    12541 cgtcccaggc ctggtcgaga ccggctcgct cacgtattgg atcatcgcga tcgtcgcggc
    12601 gggcaccggg ttctacacgt cactgagcgg cacccgaaat cctggctcca tgttgctcac
    12661 cctcggagcg aacgcgctct tcgcggtcgt gctcgcgcgg ctctacacgc cggtgttcgt
    12721 ggcgcctggt gttgcgatcg cgctcgcagc cggcggctcc ggcgatccac gcattgccgg
    12781 tcgcctcggc tcgttgaagc tgttcgtgct gacgagcgcg gtcgtgctcg taccgcttgc
    12841 cgcggagcag ctcggttggc tgcaaccgac gatgacgatg aaccccgatg ggtcgttgct
    12901 cctgcacggc ctggcgatga agggccacat tccgctcacg ccgatcatcg tggtggccgt
    12961 cctctcggtg atcggcatcg ccgtcctcgt cggccatatc gaacgcgaga gcgcgtgggc
    13021 aatgcggcgc gcatttcacg tccaggcatg gcaggtgcgt cagctgatgc ccgacatgcg
    13081 ccggaaatga gtatcctcgg cgcgatgcag gtcattcgcg gtcacgagcg cgctccagcg
    13141 tggcgcgaag gcaccgcggt tgcgatcggt aacttcgatg gcgtgcatct cggccatcgc
    13201 gcattgatcg ctcgcgcgcg tgcacttgca gacgcgcatg gagcgcaggt cgtcgcgctg
    13261 acgttcgatc cacatccgca ggcgctcttc gccgcgagcc cgccgccgat gatcagctcg
    13321 ctcgcgcgtc gcatcgaatt gctcggcgag gcgggcgctg atgcagtggt cgtcgaaccg
    13381 ttcacgcgcg cactcgcaag caaagcgccg tacgcgttca tcgacgacat cgtgatccaa
    13441 gcgctcggcg ctcgcgcgat cgtcgtcggc tacgacttct cgtacgggca gggccgcacc
    13501 ggcaccaccg acgcactgcg ctcgcacggc ggaagagcag gcgtcgaggt cgcgatcgta
    13561 tcggcggtcg atagcggcgg tgaggtgtgc tcgtcgacgc gcatccgcga gcacctccgc
    13621 gccggagagc tgaaagccgc cgaacgcctg ctcgggcggc ggtgggacgt cgacggcgtg
    13681 gtcgtccacg gcgcaaaacg cggccgcgcg atcggtgtgc cgaccgcgaa catcaagcca
    13741 gagagcgatc tcccgctgcg tccgggcatc tatgcggtga cgctctcggt cgacggcgga
    13801 ccgcagcatc ccgcggtcgc cagcctcgga acaaacccga cgttcgtcga gcacggaggc
    13861 agcctcgttc tcgaggttca cgtcctcgat ttcgacgagg acatctacgg caaacgcacc
    13921 cgaacgacct tcgtgcagca cctgcgcgat gaagcgaaat tcgattccgt cgatgcgctc
    13981 gtcgtgcaaa tccgcgccga catcgacgca gcgcgccgcg ttctctgaga cgcccgggta
    14041 gcagcgactg gtctcatgac agtcggcgga tgtcatgtcc gctgcatcga cgcggtgttg
    14101 agcatcgtgt gtgatgcgct cgcttctaag gggaggctct catgcgtttc cgtactctcg
    14161 cgatcgctac cgccctgtac gccgggctga cgcaaaccgg cgctgcatat gttgcgcaga
    14221 agggcgccga gcgcccgacc gttgctgctg gacgtcccgc gcgtctgcac cgcgacgttg
    14281 catgggcggc gccgcaaggc gcgcttgccg gcttgccgag ctggcgagtg atctgggatc
    14341 gcgacaccga cgttccgctt cgaatgtggg gaccgccgat ctccgcaccg aatgcgaacc
    14401 gcgacgcagc tgccgccgaa acagccgcac gcgcattcct tgctcagcac gggtcgatgc
    14461 tcgcgcccgg agcgaacgcc ggtgacttca cgatgctcgt caatcgcgtc gacggcgcgc
    14521 ttcgcacggt gacgttcgcg cagcacgcaa acggcctgcg cgtcgttggc ggctcgctcg
    14581 cgttcacgtt cagccacgac cgcctcatca tggtcagctc gaccgcgctg ccaaacgtga
    14641 gcgttcgcat gccgggtggc tcgctgccgt tcgcgacgct cgcgtcgacg gcgagagatt
    14701 ggctcggtca ggccggccac tctgtcgacg tcaaggcgca aggcgaccgc gtgatcattc
    14761 cgatcgtgca cgcgcgcggc aatcgcggaa cgccgcagat cacgtacaag gttgcggaga
    14821 cgctcaccgt cgagtcgacg cgtgaagagg ccggccgctg ggatgtgtgg ctcgatgctg
    14881 cgaacgcggc gccgatcgcg cgtcatcaga cgctgatgtt cgcatcggcg aagatcctgt
    14941 tcgacaccgg cgatcgttgg ccgatgggca cgcgtagtcc gaaggccgcg cccgaggtga
    15001 accataccgt cgtctcgagc gccaccgcgg tgacgagcct catggacgga acgatcacgt
    15061 gggccggcga tgccgctgct caagtcacga tcggtctcgt cggtccgcac atcaagatca
    15121 cgaacaagca aggcgcgctc gcgactgaca ccctcacgct gcagcccgat cagacgtacg
    15181 tgtggagcaa ggcgagcgaa gagttcggcg atgcgcagct gacgtcattc gtcgccgcga
    15241 gccaagcgaa ggcattcgta cgcacgcgca tcaatccgaa cctcgcctgg ctgaacaatc
    15301 agctgccggt ctcggtcaac gagaaccaga cctgtaacgc gttctcgacg ggcaacgaca
    15361 tccacttcta caaaggtggc tcgcagtgcg agaacaccgg ccgcatcgcc gacgtcgtct
    15421 atcacgagtt cggccactcg gtgcacgcgc agtcgatcat cgacggcgaa ggctcgtttg
    15481 actcgtcgct ctcggaaggt ctcgccgaca cgctcgccgc ggccatcacg ggcgaccacg
    15541 gcatgggtcg cggcttcttc cacaacgact cgccgcttcg cgatctcgat cccgtcggca
    15601 aagagctgaa gtggcccgac gacgccgacg gcgaaccgca cgacgaaggc gagatcatcg
    15661 gcggcacgct gtgggacctg cgcacggcac tcgaggcgca gtacggccaa gaggcgggct
    15721 tcaacaagtt cctcgtcctc tattacggca tcgtccagcg cgcatcggac atcccgtcgt
    15781 cgtatgcggc ggcgctgacc gtcgacgatg atgacggcga cctgtcgaac ggcacgccga
    15841 accaatgcct catcgacaac gcgttcggca agcacggtct ctccgatccg acggtcacgc
    15901 tcggcctcac ggcgccggtg cgcgaaggct tccggatctc gttcgagctc cacccgccga
    15961 cgcagacggc gtgtccccca cccggcgtct cgaaggcgac gctgacctgg gcaccgcgtg
    16021 gtggcaccgc ggccgaagtt gccgtcaccg aatctggtgg cctctggagc gcaaacatcc
    16081 cgacgcagcc agacggcacc gtcgtgaact atcacgtgac cgttcagctc gaggatggca
    16141 gcagcatcag ctacccgcag aaccccgcgg acccggacta ccaattctac gtcggtaacg
    16201 tcgaagagat ctggtgcgca aacttcgaag acggcatcgg tgagtggacc gtcggcggaa
    16261 caccagcgca gcgcaacgag tgggaagccg gagcgcctgc aggtgtcggc ggcgatccga
    16321 aggccgcgta cgaaggcaac ggcgtcctcg gtatcgacat cggcgatcag gacgacggtc
    16381 tctacaaccc gcgcaccacg cagtttgtcg agtcgccggt gatcgatctc ggcggcgaga
    16441 cgaatgtgcg tctgcagtat taccgctggc tcaacgccga agacgccgcg tacgacaagg
    16501 cgacgatctt cgcgaacgac gagcaggttt ggcagaacta cgcgacgccg ggcacgatgc
    16561 ccgacaccga ggtgaaccac accgacaagg agtggcgctt ccaggacgtc gatctcaccg
    16621 cgcaagccgc agcgggatcg ctcaagctga agttccagct catcagcgac gcgggcctcg
    16681 agatgtcggg ttggaccgtc gatcacgtct gcatcgtgaa ggcgggaccg ccttcgaacc
    16741 tgtgcggtaa ccacaacgtc gacgagggcg agacctgcga cgacggcaat accgccgatg
    16801 gcgatggctg ctcggcgacg tgtggcggtg acgaagccgg cgatggcggc tgctgcagcg
    16861 cgggaacgaa ccccgctgga ccggcgctgc tctcgctcgt gacgctcggc ctcgtagtcc
    16921 tgcgtcgccg ccgccgccgc tagtcgcgcc gcgcggctcc gcgatcaggt aacgtgagcc
    16981 gatgcgccac gtcctgatcg ctctggcatt ttcggcagcg tgcacgagag ccgacaagga
    17041 acctctctac aagggcgaga tgccgatcgc gttcggcgaa tgtgccggac cgagcgtcgc
    17101 gtttgtgtcg gggccgcggc cgttgccgtt cgaagcggcg accgtcgaga acctcgtcgc
    17161 cggaaaggtc gcgaaggacg aggtgcccaa gcccgagccc gaagatccgc cgcccaacga
    17221 gaacggcggt ggcttcgcgt cgctcatcgg cacgggcgac atctcgagcg ggttcgatga
    17281 cacgaacatc tacggcgggc ttctcggcaa cgaagcggga gagatgaacg gcgggtttgg
    17341 cttcggccgc agcggctttg gtccgggcgg cggtgggcaa ggcatcggca cgatcggcac
    17401 cggccgttac ggcacgatcg gccacggctc cggcaccgga tcgggttacg gcgtcggcgg
    17461 cgggcgcggc gggatgcgcg ggcgaacgtc cgcggtgcct acggtttcga tcggccaacc
    17521 gaacgcgcaa ggcgatctcg ataaagcgat catccgtcgc tacatcaagc gcaacatcca
    17581 gaagatcacg tactgctacg agaagcagct gctcgtgaag ccgaagctcg ccggcacagt
    17641 gcagacgcag ttcttcatca cgccgaacgg caatgtcgcg aagtcgagtg gcagcggcgt
    17701 cgatccggag gtcgcggact gcgtcgcggg cgtgatcaag gacatcgagt ttccgaagcc
    17761 caaaggcggt ggcggcgtgc aggtgaacta tcccttcacg tttcgtcccg cgggcgacac
    17821 gacgacgatc gccgacgcgc cggcagcggg cgctgggagc gatgcgggct ctgccgctgc
    17881 gcctcccgca ccggatgcgg gctctgcggc agcggcgcca gctccgacgg gagagctacg
    17941 ccacgtgttc cgcgcgtggg gaacgccggt cccagatgcg tcgaagtacg atccaggtgc
    18001 gaacaatccg ctgcgcgcgc tcgagaagga cctcagccag tgtttccgcc gcggaccgaa
    18061 tcacttcgga gccgtcgtcg tcgagctgac gtacgacgcg agcggcaaga cgacgaacgc
    18121 ggtgccacac ggagtgacgc acgagagcgc ccgcaagtgt gtcgagaagg tcgcgaagcg
    18181 tgtcacgcgt ggtgcggggg cggcgacgac gcagcggtgc tcgctgtcgt tcggcgtcat
    18241 gcctgtcagc gcactacctg ccatcgacat cacatcgaag ggcattgcgc tcgacgggcg
    18301 ctcgctcatc acgatcgcgg acgccgacac gcaatcgccg ggcctcgcga ggaccatccc
    18361 gtcggtgcga gaagcgatcg aagcgcgcgt tgacgctgtc acgcaaaccg accgcgtggt
    18421 caccgcgcat gggccgctcg tgatcaagcc gttgccgaag acgccgatga agtacgtcga
    18481 tggcgttgcg aggagcgtgc tgcgcggcgg cgacgacttc gtgctcgcga cctcggacgg
    18541 cagcgaatgg aagctgctct cgccgttgac gcttccactc gtgccggtgc cgttcagctc
    18601 gggcggccgc tggaaccgct acaagggccg gttcctcgac ggcacggtgt cgcaggacga
    18661 cgaacgcgtc gtgctctcga tcttgatcag ttccgatcgc atgtggctcg gcctatcacg
    18721 cgtgaacgag ttccaagagc tcccggcagg tcccgatcag ctcggcaagc tggccgccgc
    18781 gctgaaggcg cagaaagcga gcgcattctt cgtcgatcgc gacgacatcg agatcgccgg
    18841 cgacagcgac gtgccgtacg agacgttcgt gaagatcgtc gaggccgcag aagcggcggg
    18901 cttcaagacg tggcagcaca ccagtcccga cgggctcgca gcgcggcccc agctctagcg
    18961 cctgcccccg ccgctgagtc gcgacggaaa tcgcgttcag tcagcggttt gtcgtgattt
    19021 gcgtgaagtt ttgagaatct catcgcgtcg taggtgccca tgacgctcgt acgacgctcc
    19081 gctggcttcc tcgtcgctct cgccctcgtc accgcgtgcg aaaagaaacg cgaatcgcac
    19141 gctagcctcg cgcagaagga acgcgagagg caggcgaggc tcgtcattcc cgatctcacc
    19201 gagccggaac acgcgccgcc gccgaccgcg ccgcccgcgc ccgaagcacc gtcgcccgac
    19261 gccatccgca agctccacgc cgagcccatc gcaggggtca acctcggcat gacagacgcc
    19321 caggtgacaa agatcctcgg tgcaccgacg aacaagtcgg cgatcgccgc gctcggcaac
    19381 acgaagaacg aattcggcat gacgtggacc tggccgacga tgaaggccga gctggcgggc
    19441 gcaacaaaga aggggccgtt cacgctccga agcatcacgt tcttgcgagc aagcaaggtc
    19501 aagacatcgc gcggagtcgg catcggaagc acgcgagccg aaatcgcctc cgcctacgcc
    19561 ggcctcgaag atccgatctt tgccaaggac acgcggatgt tcgtggtggg cgcgtcgacc
    19621 agctacggca tgaacggcat cagcttcaac ttccgcgacg acgatcgcga cggcaacctc
    19681 gagacggatc gcgtgaaaga gatcgagtgg gccggcggcg ccgagcagta gcgcatcgcg
    19741 acccgacgtt cacaaccggc gcgcagcgcg aaacgaacgc gcataaagcc cggtgccgtc
    19801 gagcgtggat gcaccgctga agtcgccaaa cgtcggtccg ttgatcgact tgcgactgga
    19861 gatgaaacgc ggatgaccgt cgacatcgat gccgaggtac atgccgacat gatcgattgc
    19921 ggtgccgtcg tcggtcgacg catcgaagaa cacgaggtcg cccacgaaca gccgatcgac
    19981 gtcctcgctc gcgtgcgcgt cgacatcgat cgtcacgacg ccgggccccg acgctaagat
    20041 ctggaccgct cgacgcggca tcgcgcgccc gtcaccatcg gcaccagccg tcaggggaac
    20101 gtgatggcga tagccccata ccatccgcat gaagcccgag cagtcgaggc tgtgaagctg
    20161 cgcggcctcg ttcgtgtcgc gtgtgccgtc ggaataggtc cacgggatgc ctaggtagtc
    20221 gttgaagtcg gagccttcct gccgcgcgcc gtcgacgagc gggccgtagc tcgcgtcgcc
    20281 ggcgatctgc aagccggtcg tgttgctcgt gattggcggc gcgccttcag cgtattgcag
    20341 cgcgcgagcg atgacatcgt tcgacgtgtc cactcgggcg gcgtcgagcc acgcgacgtc
    20401 gacggtgccg ccgaatggtg tatcgagcac gcgcacccag tgcgtattgc gaatgtcgat
    20461 cgcggtgcgc tcgtgcagcg ttcgcgatgg ccccttgacc cgcacagttc gtgcgccgtc
    20521 ggtgaacgtg gctacccagt gaccattcgc atcgtagacc gcggtgcgcg gcggcaagtg
    20581 aaagcgctgc gtgctgtagc gaccgttcgc gagctgcgcc tcgatcaccg ccagctcgtc
    20641 ctcggacggc tcaccgacgg cgcacgcggt cgcgaaaact cccacgagtg aacagacgac
    20701 gacgagacgc atgctgttgc cgcgtgcaac gagcaagccg cgatccggta gtgcgacctg
    20761 aggtccgttc tcgcgcaatc acgcaatctc gaacgggact gaacgaggct ggcacgtcga
    20821 tgcccaggcg gctcgcgtag ccatggctcc tcggggcacc gctcagcggc ttcccggcac
    20881 gaaccagcgc gtgaatcgct ccggcgacgc gacaccttca tcgcgcatcg tcttctcggc
    20941 ggcggcaatg tgcgcggcgc cttcgctcgc accgaggagg acgccgagct ggcggcgcgc
    21001 ggcggccgcg tgcagcgaga gatcgagctc gtcggcgcgg gtgtacgcag cgcgcagctg
    21061 cgcgattgcg acgtcatcgt tcccggcgcg catcgcgagg cctgcgcgga ggagcgcgcc
    21121 gaacacatcg acgcgtgggt tgtcgacctt cggcaggttc gcgatcgcgc gctcgacgag
    21181 cttttgcgcg tcgctgcctt cggtgcacag cgcgaggcgg ccgcgcagcc agagcgacag
    21241 cgcgctgacc gtcgtgacgc gcgtgagcac gctcttcgcg agaccgtcga acagcggttc
    21301 gagcgtcggc atgtcgcgcg cgacgctgcc ttcgtagagc gctatctccc cgcgcgcttc
    21361 gagctcgtac cagtgctgtg cgtggaatgc agtcgtcggc aatacccacg tcgcgttgtc
    21421 gaccatgttg cgcgcgcgcc gcgtgtcgtc ggcggcgagg tagagcgcgg agcagtagcg
    21481 gttcatcgac gtgatgacgt agcgatcgcc gcggcgctct gcatcggcga cgtagcgttc
    21541 gcgcagatcg cgaaggcgag cccacgcacc gcggtgacgc agcgcgaatg cgtggaacat
    21601 gcgcgcgctc ttgagctcga ggttcgtgcc ggtcgtttgc tcgcggaaca ccgcttcggc
    21661 ttggcctatg agctcgtcgg cgcgagcgag ccggcacgtc caatactcga cggtgccttc
    21721 agacatcagc aggaatgcgc ggaggcgggg gtcgttcgat tcctggcaga ctttgcggac
    21781 gctatcgagc aatgcgcgcg agcgaacgac gccacggccg ccttgcgacg actggtagac
    21841 cgtctcggtc agaaccgcga gagcggtgcg gatgttctcg ccgatctgca gcgcgagaag
    21901 cagccagcgc gcgttgaagg cggcggctcg gatgttgtcg acgagggcaa gactctggct
    21961 cgccgctttc agcacgtcga ggcgcagcag cgtctcgggc gcgatctgac tctcgcggcg
    22021 ctccttccac cgcaggccgc gcatgcgcaa cttcgcgcgc gtccagacga ccgatgcaag
    22081 tgcactcttc tgcgtcttcg gcatcttcac gccaacgtcg gcgagcagcg cgcgcaagat
    22141 ctcgaggcct cgatcgatct cgccgaccga gagcagctgc tctgcagcct ggcgatgaca
    22201 cgacagccgc gtcgcaggat ccgcgtcgcg cgcgacgtcg aggaacgctt cggcggcttc
    22261 cgcgccgcga cccgcgttcg cgagcgcttg gcccatttcg gtgcgtagcg cacgcgcgcg
    22321 ttgcgcatcg acgtgcgagc cgcgcagtgc gagtgcgaac aagctcgctg cttgatcgaa
    22381 cgcgcccgcg ctcactgcac gctgtgctgc ttccccggca agctcggccg cgcgtgtgct
    22441 ctcgcccgcc gcttcgagat gccagatgag cagctcgggg cgcgctcgcg cgagtggcga
    22501 tgcttcgagc gccgacgcga gcttcttgtg ccacgcgctg cgctcggcgg gcggcatcgc
    22561 gtcgaggacg gcttgccgca cgcgatcgtg atacggctcg agcgagccat cggcgtgacc
    22621 gctgcgcacg agatgcgcga tgcgcagcga agcagactgc cgctgcacga cggacatctt
    22681 ctcgtcgagt gcgtcgcgta cgacttcgag cgtgatcggc tcgccggcta ccgcaagcac
    22741 gtcgacgatc gcgcgcgctt ccggagggag gccggcgatg cgcgcgagga tcgcttcctc
    22801 gagtcgcacc gtcgcggcag catccgcgct gccctttgtc gcagcgtagc ggacgagctc
    22861 cgagatgtag agcgggtgcc cgccggtctc gcgtgcgatc gcgtcgaggt cgagctgcag
    22921 ctgaggcgcg aatgcggcac gcagctgtcc ggcgagctcg atcgccgggg caagcgcgag
    22981 tgactcgagc gagatgcggc gaacctccgc cgtcttcacc gcctcgatgc gagccgccgt
    23041 ctctgcgtcg tcagacggcc gcgccgtgag cacgagcaag atcggcggcg catcgcgatg
    23101 acgcacgaga tcgcgtgcga gcacgacgct gtcaggatcc gcccattgcc agtcgtcgat
    23161 cgtgagcacg agccggcgcc gcgacgataa tcggtgcagc acatcgcgca gcgccgcgaa
    23221 cgcttggttg cgctgctcgt gcgggtcggt cacgggcgtg cgcgccgtcg gcgcagcggc
    23281 gatcgcgggc accgcgccaa gcaccgggaa gatgcgcacg agtagatctg gatgccgcgg
    23341 gaggagcgc
//
LOCUS       DATLBZ010000194         3225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9784775, whole genome shotgun sequence.
ACCESSION   DATLBZ010000194 DATLBZ010000000
VERSION     DATLBZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9784775"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VMZ53_10170"
     CDS             <1..370
                     /locus_tag="VMZ53_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUS28869.1"
                     /translation="QYRSSATIFSEGEPGDKFYMVLEGAVRISRIVPGMGEEALAVLR
                     PGNYFGEMSLIDDAPRSATAMCHEKCRLFVVNRRDLEDLLFVDRDLAYELLWNWVRTL
                     SRRLRATNDKMTFLATTSKF"
     gene            371..940
                     /locus_tag="VMZ53_10175"
     CDS             371..940
                     /locus_tag="VMZ53_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28870.1"
                     /translation="MRALAAAFVLVCALAARSHAEPLPPGTIGPVFGVLSGTGADAKR
                     IGYGMYPFGMQASWQPMTTERTRGWTLRWSTMFGRLYNGSASQIDSVLLTVQMDIMLG
                     IRYRPWSSPRRYLTLRAGGELLRSNQPIPRSPDDDTKQRNFLGGVASVGYDHYFGGFL
                     MWNVDVRFGLAGTGPSQASVLVGIGFTGP"
     gene            940..1503
                     /locus_tag="VMZ53_10180"
     CDS             940..1503
                     /locus_tag="VMZ53_10180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HUS28871.1"
                     /translation="MPVLVTQRLRLVPITLGMIEGVMKRDRAAAEAALDEELRRSNIH
                     ADACARFPDQWPNEELVARAFPFTLDAIRADPDTRLWGDSLVLPLEGPPRVLGSVVFK
                     GRPADGIAEVAYGIEDGSRSQGLATEATRACVDWALEQDGIHAVQATTFPWHHASLAV
                     IRKLGMSQCHTREHDVFGELLVFERRR"
     gene            complement(1500..2126)
                     /locus_tag="VMZ53_10185"
     CDS             complement(1500..2126)
                     /locus_tag="VMZ53_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28872.1"
                     /translation="MRTLATIFVVFVAACTGEVTNPGVSNEEMAGAGGRNIAEYWPSI
                     DVPLSQNTRMLGFDMMRSEVKRVTGRSWVVAGVDQWERNRGSLGGADFETTFSDDLTP
                     SQQRLVLVRKMAFQVCGDLVAAEAGVATREVFTELDPGAAFDPASATTEAQIRALFKR
                     FFLEEPAADDISDAKALLATLAPGGTAGRTAWRGLCTSYLASMRFLTY"
     gene            complement(2114..3079)
                     /locus_tag="VMZ53_10190"
     CDS             complement(2114..3079)
                     /locus_tag="VMZ53_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28873.1"
                     /translation="MRILSISIIAVLAACVSPAPAPGGGGDDSSGGTATTRTSDLVCA
                     RKMALDLTHNAVAPADLAKLAQHDITLAGLADQYLASPEFETVAFDWMRAEFPPTALT
                     NAMTDVEEPARIMHHVLMADRDWHEILTATYTVDATGTEVPITDRPAAGIISTKYSLQ
                     STVGSFRRNWAGRFERQFAGITLVAVTLPPGDMTDVTPDGLAGNPNCAGCHVHPTYGI
                     DRLAKFAACYDDDGARKAGCEDPEASFLLQTGHGLADLGRIAAYTNEFKSQSINFFFK
                     KLFGRDLAKEEAGFYADAARHFTTSGFKARALIKYLVTTEAYCAR"
BASE COUNT          544 a         1037 c         1073 g          571 t
ORIGIN      
        1 gcagtaccgg tcgtcggcga cgatcttttc ggaaggcgag cccggcgaca agttctacat
       61 ggtgctcgaa ggcgcggtgc gcatcagccg catcgtcccc ggcatgggtg aagaagcact
      121 cgcggtgctg cgtcccggca actacttcgg cgagatgtcg ctgatcgatg atgcgccgcg
      181 ctccgcgact gcgatgtgtc acgagaagtg ccgcctcttc gtcgtcaatc ggcgcgacct
      241 cgaggacctc ctgttcgtcg atcgcgatct cgcgtacgag ctgctctgga actgggtgcg
      301 cacgctctcg cgtcgtctgc gtgcgacgaa cgacaagatg acgttcctcg cgacgacgtc
      361 gaaattctaa atgcgggcgc tggccgccgc gttcgttctc gtgtgtgcgc tcgccgcgcg
      421 ttcgcacgcg gagccgctgc ctcctggcac gatcggtccg gtgttcggcg tgctgtccgg
      481 caccggcgcg gatgccaagc gcatcggcta cggcatgtac ccgttcggca tgcaggcgag
      541 ctggcaaccg atgacgaccg agcgcacgcg cgggtggacg ctgcgctggt cgacgatgtt
      601 cggccggctc tacaacggca gcgcatcgca gatcgattcc gtcctgctca ccgtgcagat
      661 ggacatcatg ctcggcattc gctatcgccc gtggtcgagc ccgcggcgct atctcacgct
      721 gcgcgcaggt ggcgagctgc tgcgctcgaa ccagccgatt ccacgctctc ccgacgacga
      781 tacgaagcag cgaaacttcc tcggtggcgt cgcgagcgtc ggttacgatc actacttcgg
      841 cgggttcctg atgtggaacg tggacgtgcg cttcggactc gcgggtaccg gcccctctca
      901 ggcatcggtg ctcgtcggca ttggattcac ggggccctga tgccggtgct cgtgacgcag
      961 cgccttcgcc tcgtgccgat cacgctcggc atgatcgagg gcgtgatgaa gcgcgatcgc
     1021 gccgccgccg aagccgcgct cgatgaagag ctgcgtcgct cgaacatcca cgcagacgcg
     1081 tgcgcacgct tccccgatca atggccgaac gaggagctcg tcgcgcgcgc gttcccattc
     1141 acgctcgacg cgatccgcgc ggatccagac acgcgcctat ggggcgactc gctcgtgctg
     1201 ccgctcgaag gcccaccgcg cgtcctcggc agcgtcgtgt tcaaaggccg cccggccgac
     1261 ggcatcgccg aagtcgcgta cggcatcgag gacggctcgc gcagccaagg cctcgcgacg
     1321 gaagcgacgc gcgcatgcgt cgactgggca ctcgagcaag atggcattca cgccgtgcaa
     1381 gcgacgacgt ttccctggca tcacgcatcg ctcgcggtca tccgcaagct cggcatgagc
     1441 cagtgccaca cgcgcgagca cgacgtgttc ggcgagttac tcgtgttcga acgccgtcgc
     1501 taataggtca ggaagcgcat cgacgcgaga tagctcgtgc agaggcctcg ccacgccgtg
     1561 cgtcctgcag taccgccggg tgcgagtgtc gcgagcagcg ccttggcgtc ggagatgtcg
     1621 tcggcagcgg gctcctcgag gaagaagcgt ttgaacagcg cgcggatctg cgcttcggtc
     1681 gtcgcgcttg ccggatcgaa tgcggcgccg ggatcgagct cggtgaagac ttcgcgcgtc
     1741 gcaacgccgg cttccgccgc gacgagatcg ccgcagacct ggaacgccat cttgcggacg
     1801 agcacgaggc gctgttggct cggcgtcaga tcgtcggaga acgtcgtctc gaagtcagcg
     1861 ccgccgaggg agccgcggtt gcgttcccac tggtcgacgc ccgcgacaac ccagcttcgg
     1921 cccgtgacgc gtttgacctc gctgcgcatc atgtcgaagc cgagcatgcg cgtgttctgc
     1981 gagagcggaa cgtcgatcga tggccagtac tcggcgatgt tgcgaccgcc cgcacctgcc
     2041 atctcctcgt tggagacgcc gggattcgta acctcgccgg tgcaagcggc gacgaagacg
     2101 acgaagatgg tggctagcgt gcgcaatagg cctccgtagt cacgagatac ttgatgaggg
     2161 cgcgagcctt gaacccggac gtggtgaagt gacgggcggc atcggcgtag aacccggctt
     2221 cttccttcgc gagatcgcgg ccgaacaatt tcttgaagaa gaagttgatc gactgcgact
     2281 tgaactcgtt cgtgtaagcg gcgatgcgcc cgagatccgc gaggccgtgg ccggtctgca
     2341 gcaagaacga cgcttccgga tcctcgcagc ccgccttgcg tgcgccgtcg tcgtcatagc
     2401 aggcggcgaa tttcgcgagg cgatcgatgc cgtacgtcgg atgcacgtga caacccgcgc
     2461 aattgggatt gccggcgagg ccatctgggg tgacgtccgt catgtcgccc ggcggcagcg
     2521 taacggcaac cagcgtgatg ccggcgaact gacgctcgaa gcgacctgcc cagttgcggc
     2581 ggaacgagcc gaccgtgctc tggagcgagt acttcgtcga gatgatgccg gcggcgggac
     2641 gatcggtgat cggaacttcg gtgcccgttg cgtcgacggt atacgtcgcg gtcagaatct
     2701 cgtgccaatc acgatcggcc atcagcacgt gatgcatgat ccgcgcgggc tcctcgacgt
     2761 cggtcatcgc attcgtgagc gcggtcggcg gaaattcggc gcgcatccag tcgaacgcga
     2821 cggtctcgaa ctccgggctc gccaagtatt gatcagcgag cccagcgagc gtgatgtcat
     2881 gctgtgcgag cttggcgagg tccgccggag ccaccgcgtt gtgcgtcaga tcgagagcca
     2941 tcttgcgcgc gcagaccaag tcggacgtgc gcgtggtggc cgttccgccg gacgagtcgt
     3001 cgccaccgcc gccgggtgcc ggagcgggcg agacgcacgc cgcgagcacg gcgatgatcg
     3061 agatggaaag gattcgcatc gctacctcac gcctggatca cagcgttgat cggtttcgca
     3121 ttcgagtacg tcgaaggatc gacaccattg gctttcaaga tcgtcgcgac gacgttcatc
     3181 cacgtcggca ccgagccctg tcccatcgcg cccgtatcga agttc
//
LOCUS       DATLBZ010000195        16605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1681390, whole genome shotgun sequence.
ACCESSION   DATLBZ010000195 DATLBZ010000000
VERSION     DATLBZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16605
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681390"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VMZ53_10195"
     CDS             <1..688
                     /locus_tag="VMZ53_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUS28874.1"
                     /translation="CLFVTGDIGEADSVGRFVEELGRIAPVYYVLGNHDYYRASIHAV
                     RENLPAGWLPAAGPVALTEHTVLLGVDGWGDARCGDLDSNVQLSDWQLIEDFERVRYD
                     RKARVALLQELGTAEAFVLGELLARAPASQRLVVLTHVPPFPEACVYDGAQSEPAWLP
                     WFTCIATGQLLAEYARAHPQKQITVLCGHSHGAGVCRPEPNLEVRTGGWPPGVEGYGN
                     PIVQATLELP"
     gene            complement(691..1335)
                     /locus_tag="VMZ53_10200"
     CDS             complement(691..1335)
                     /locus_tag="VMZ53_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28875.1"
                     /translation="MRVLPLLLALAACKSGGSPRGAGAGFDDVTYHTSLAEDDGGEPA
                     PSYDKGEVQKALFAERAAEAKWELRITELESAGDPDPVNAARADLAVRRRFIATLETC
                     EATNHYCPPRLDDPPWNYAVDSDADPKLDAPLRFDLASWQKVSAELHGRACACRTLTC
                     VDSVNAAIARLETRPMEDVRGDETSSIEITRARDCIYRLLGKRSLPRVETATAE"
     gene            1425..2411
                     /locus_tag="VMZ53_10205"
     CDS             1425..2411
                     /locus_tag="VMZ53_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HUS28876.1"
                     /translation="MIDIIGILTRTWLGVHSPAPQQPAQPVPAVARAKQHVMTAPLIG
                     TPAPAAAATGSAAPVPAKTPATAPAKTPAPAKTPAATPAKGSGAADAKTPAPAADPKT
                     VPATAPANADAVVTEVQKFYAGIKQVTAQFRQSVTNNTFGSTKTSDGTVWLMKPGKMR
                     WDYVEKKKDKVQVKKSFISNGTYLYVVEHDNKQVVKKNLANDLMPVAVTFLYGKGNLS
                     AEFNAAIDTTGKYGAKDDIVLKMSPKKPSAQYKALFLVVAKDNYRVKQSVIVDSSDNV
                     NQFMFFGPDFEKSIKDSWFEFDERSVKNYRIIDADQPQGSGAAPAALPPPAK"
     gene            complement(2657..2959)
                     /locus_tag="VMZ53_10210"
     CDS             complement(2657..2959)
                     /locus_tag="VMZ53_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28877.1"
                     /translation="MRNVLFGVVLAVAAASAGCKKADHSAAKAEEQFATLTVDEVAAQ
                     LEAKQIVPVDCNGDGTRKQMGVVPGAVLVTDEEQYGPSELPADKTTKLVFYCSKET"
     gene            2982..3812
                     /locus_tag="VMZ53_10215"
     CDS             2982..3812
                     /locus_tag="VMZ53_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS28878.1"
                     /translation="METVRANGLQFAYLSEGTGPLVLLLHGFPDTAHTWDRTIKELAA
                     AGFRAVAPFMRGYAPTEIPADGDYSVDTLGRDALALIEALGEKTAILVGHDWGAAAAY
                     AAAGLGPERISLLVTVAIPHPKSLKPNPLLLLKQRHFFALRGKKAGARVREKDFAYVD
                     ELWRRWSPAWKDIPASETAAVKAAFADAGRAEAAAAYYSFVGFRPPKSYDAKVKMPAV
                     AFAGEHDVISSRAYEKARHCYEKSYEVVIVPGGHFMHREHPDAFVPELVRVVSDHAKR
                     "
     gene            complement(3802..4557)
                     /locus_tag="VMZ53_10220"
     CDS             complement(3802..4557)
                     /locus_tag="VMZ53_10220"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase family protein"
                     /protein_id="HUS28879.1"
                     /translation="MIAAFDRGDWPGVLELVAPNARAYIGGTTADREGWRQFGQMWSA
                     AFPDTKHEIVAAHVAGEYATVVCTLRATHKGDFMGIPATGRSITMEVIHVDRVVDGRI
                     VEHRGQFDSAGLMQQLAGPPSDHRAFLVNVFELIDSQKFEAAKAQMLPNARFVMGGQA
                     MTPDEWAGFSQAFFAAFPDGRHVHDQIFSAGDRVIHTGSFEGTQRGDFQGIKATNRRV
                     SFTYIGIMTVQNGKVLEERVEANFAGLVQQLTA"
     gene            complement(4643..6169)
                     /locus_tag="VMZ53_10225"
     CDS             complement(4643..6169)
                     /locus_tag="VMZ53_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUS28880.1"
                     /translation="MRLAELVGTLSLATDAGTAMPEESGLMAAIAAAKLGEVIGATPR
                     EQSDAYYLALLRYSGCTADNDIAFSLFGDELQFGRDTYGLDYGDARQVLPAVLRSARR
                     GKGVVGGAMAMARALGKLPKMPAVSRAHCEVADLLAARLGMDATLRAALVQHSERWNG
                     SGMPAKLKGDAIALTMRLVHVAFDVVIGFKLGGAEGAVSRVKQLSKKGLDPNIVEKFV
                     ALSDRVVSAIDVPSIWKAFLEAEPAPQKEVNGDAIDEALATMAAFADLKSKYTRGHST
                     AVSELAAAAGKAAGLDEAMQRDLRRAGLLHDIGRVAITSGIWDKAEPLTDLEREKIRL
                     HTYIGERVLARAPALAAIAELSCLAHERLDGAGYHRRLSAAACPPAARILAAADVYVA
                     LGEDRPHRKAMTREDAAAEMTKMAEGGSLCPDAARAVLAAAGHVVKKVDRAHGLTDRE
                     IDVLRLIAKGFTNKEIASSLEISTKTAGHHVQHIFEKLGVTTRAAAAICAMSKGLTSA
                     "
     gene            6220..6738
                     /locus_tag="VMZ53_10230"
     CDS             6220..6738
                     /locus_tag="VMZ53_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUS28881.1"
                     /translation="MLVRIGARTNTEDVVDLLAGCHARIRQFLTMSQRLATDAASDDD
                     VRDTAAQIHRYFTSAFLDHVLDEDELITPRLVGRSDKLDAILAAMHRDHAQHAGLIRR
                     LVSECEIIEKDPRLAGALGAELLDIAKLLEAELVPHLELEERELFPAIRGLPQSVRDE
                     IREAMRQRREQR"
     gene            complement(6735..7352)
                     /locus_tag="VMZ53_10235"
     CDS             complement(6735..7352)
                     /locus_tag="VMZ53_10235"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS28882.1"
                     /translation="MATPGMDKRKHPRFGVRLAVRYTNAEQFVTDYVENLSAGGLFIK
                     GAHQLPMHTETDVQVELPGQGNWSVRAKVVFLIDPTAAETTGRTPGAGMQIVDKPPGF
                     DDALLGYLLRLGRRRDHAVMLGEGVIGAQLFEDAGYRVMPLESEDEVAFAMANGDSAI
                     VAIVLSPTLVQTYQDRLGERGKSVVFSAISIEDVHDILARIDSLL"
     gene            7410..8633
                     /locus_tag="VMZ53_10240"
     CDS             7410..8633
                     /locus_tag="VMZ53_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28883.1"
                     /translation="MAERPLLIFVSDIHLTDTLHANAVSKAEQFGRFWDRIQVARGKR
                     PAELCVVGDLFDLVRSPSWFDGRHRPYHGASNNGVVKNVEQIVEACIAREQGFFDALK
                     AKIKSGELVLHYVVGNHDRLLLTSPGAQKKIADALGLPKLVLHRELAFPDHGVLAYHG
                     NVGDPINASPEGDATIGDAIGSELILKFPRKLRALVGADHPGVEEIDDIDDVRPIYAV
                     PAWVRQQSAIRKDLLRPISQVWSEVVDEFLANDFVKQWLKTQNKLFSLDLGKKLRLLL
                     ELSRNKVMAHGSDERLSQLYRFFQHSFDGKMQQVAAEELLKRKGLRYVVNGHSHFPSM
                     QPLGRIGDAASVYFNTGTWRAVHQIGHDLGGRPSFVQYDAMTYLVFFPTDDKLGRDYE
                     WWTGAMVTREQALHA"
     gene            complement(8630..8995)
                     /locus_tag="VMZ53_10245"
     CDS             complement(8630..8995)
                     /locus_tag="VMZ53_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HUS28884.1"
                     /translation="MCPSCGIELVLSGTRLHCKQCNGMLVRSEEVEEMMQHMAPDQPR
                     TISKLIRAGGSDAPRKCPSCDVMMTVCLLGPVTVDQCPEHGIWFDGKELTRALEGQGS
                     PIASPTLSTLYGWWQAGKR"
     gene            9032..10678
                     /gene="gpmI"
                     /locus_tag="VMZ53_10250"
     CDS             9032..10678
                     /gene="gpmI"
                     /locus_tag="VMZ53_10250"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HUS28885.1"
                     /translation="MSSSPLRPHPRHPGPKGPVVLVVMDGIGRGAGDEADAVSIASTP
                     TLDRLWVPGARAELKAHGRAVGLPTEDDMGNSEVGHNALGAGKIYAQGAMLVNRAIAS
                     GSMYEGATWQQIIARGVRGGAVHFLGLLSDGNVHSHLDHLEAMMGTAATAGCKRLYVH
                     ALLDGRDVPPTSALEYIDRTERFLAGLRASGVDARIVSGGGRMKLTMDRYEADWPMVE
                     RGWRTHVLGDGRLFSSATEAVNTYRGEFPGIIDQDLPAFVVAPTQPMQDGDSVVMFNF
                     RGDRAIEISRAFDDGEFAPFNRVRKPDVLYAGMMEYDGDLHVPRHYLVTPPQIERPMG
                     EILAAAHVSQLAISETQKYGHVTYFWNGNRSGMFDQGTETYIEIPSDRVPFEQRPWMK
                     AAEITDRLIAELRTGKHRFARVNYANGDMVGHTGAFDATVLAVEAVDLQLARLAAVVQ
                     ELQGILVVTADHGNADEMFQRDKKGVVMRDKAGHPVAKTSHTLNPVPFLIDDLARGDR
                     YEIDPERAKVAGIANVTATCIELLGFVPPDDLESSILRFR"
     gene            10675..11244
                     /locus_tag="VMZ53_10255"
     CDS             10675..11244
                     /locus_tag="VMZ53_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUS28886.1"
                     /translation="MSTKALIGILLAGTAGILIFLFVHLSGGDSPKVAIGSKEAQACA
                     KGGEDCLPDVNYVDTQGTAYTFASLHGKVVIVNFWATWCGPCKKELPELSRLYKKYKA
                     QGVVMLGVLTSDTPDEDTLLNFQSDYEMEFPIVRQTSDILVSFNYPGALPTTFVFDRH
                     GKRVGPPHVGAIRADELDAQLAQLVAQKN"
     gene            complement(11241..11795)
                     /locus_tag="VMZ53_10260"
     CDS             complement(11241..11795)
                     /locus_tag="VMZ53_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28887.1"
                     /translation="MRRIVLLAILLISTTASAGPRNRNTARVMSGTAAGVSGAITLAG
                     FITTPEGDAFNKPVLYTGIGMLMVTPSLGEMYAGQYLTWGMGVRAVATGFAIWTLQTQ
                     TEPVRCEVINAPHEPTCNSFNDMAYPLLGIAGIAFVGGVWYDVLDAADSADRFNARHG
                     YTVVPAPMQGPNGMAPGLVVSGAF"
     gene            complement(11918..12499)
                     /locus_tag="VMZ53_10265"
     CDS             complement(11918..12499)
                     /locus_tag="VMZ53_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS28888.1"
                     /translation="MKQCAIAVGLVLSACTFEAGQAPDKLVLKSSNTSLERNGHASVT
                     AAYKFEALELPADDVTWTSSDETVATLSGAGHEIALDALKPGVTRITAHATDLSSTID
                     ITVLVPVVREVSISPSSPSVAAGQNVQLSATATYSDATTADVTTKAIWISSDTSKATV
                     DRGTVHALATGGVTIRAVMDSSQSSLVVTVTAP"
     gene            complement(12589..13086)
                     /locus_tag="VMZ53_10270"
     CDS             complement(12589..13086)
                     /locus_tag="VMZ53_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28889.1"
                     /translation="MKLTTLILLALIGCGRDKSQPTPPPAAGSDVVKKPPDQMDEKMR
                     HCPLAIDGATSELRDIDGGVRFTIHPPADKLDDARKRAHHIVEFAAKRTREGHGGFDG
                     QGGGRMKNCPVVTDDVTITTRDIDGGAELDIVSASGHADDLRAATRERVSRFPFTGAT
                     ITQTP"
     gene            complement(13083..14444)
                     /locus_tag="VMZ53_10275"
     CDS             complement(13083..14444)
                     /locus_tag="VMZ53_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS28890.1"
                     /translation="MTISFRFKLLASHALVALVVSAVTLVVVERLVSRRMEQQLDHRL
                     ETQARAVATWLSRAAHPGQLAHRLADVVDARVTIIDKRGIAVGESQAAAGTPPRMDSE
                     GSPQEVADARAGAVGRETRFSSAEAQPVRYIAVPAPQESVVRLGVPIGEFEETKSQLR
                     TQLFAAALASLLLALGLAALVAGPLTRRLRDATVVAQRIGAGNYDAVQPSPSDDEVGV
                     LSRALARTATELRATEQHRREFLANVAHEVRTPVTSIRGYAELLKTSDSLDETGKEFA
                     TTIHRNALRIGQLVEDLLELEAIEAGKGAPLAAEPVALAPVVQHVIDTLKARVDESGA
                     SIRVDVSDLAPIGDEDAVERIILNLVDNALRHGGAAVQIDVAANRADNRIRIVVKDSG
                     PGIPAEDRDRMFERFHRGAAARDPQRRGTGLGLAIARELAVAMHGSLTLGEGSTFTLE
                     LPA"
     gene            14471..15172
                     /locus_tag="VMZ53_10280"
     CDS             14471..15172
                     /locus_tag="VMZ53_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS28891.1"
                     /translation="MATRVLVVDDERDVCRLLTYSLDQAGFAAASVQTAAEALIDAAR
                     HAPAVVVLDVGLPDMSGVEVCRKLRADKQLADVGIVMLTALGSREDRIVGLEAGADDY
                     VVKPFDVQEVVLRVRALSRRISERESARKGSAPSTAQALRSGTLTVDPVTHEVRGDGE
                     LLSVRPLEFKLLATLLGEPGRVYSRRELLHLVWEIEHGSERTVDVHVRRLRLNLGTWA
                     DQIETVPGFGYRAKR"
     gene            complement(15176..16231)
                     /locus_tag="VMZ53_10285"
     CDS             complement(15176..16231)
                     /locus_tag="VMZ53_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS28892.1"
                     /translation="MKWLALVLSLTGCDALFGLDDIRTEGDAAVTTARYRKRLTVQGT
                     GDLTAFPVLVWMTDPALEAHADPSGRDITFTAGDGTTPLPYEIVSHDGDTIEAWVQLT
                     LSASPTDFYMLYGGSVVQPKASPWTGPFVGVWHLSMPGSIELDRSSHQNDLSSGNAPP
                     APTQGAAGGAVTFDGTNRMCTGLGDGSLDPGMSSFSFEAWAFVPTQPTVPQVLFERGG
                     FTDGVAGFDLEIGNGNWRGYIADASTTLSAELGAVTPGKWSHLVVVVDRKASLMAAYA
                     DGAIQTTTAITLGPSINPTRPLCLGGFTSYEYRGGLDEVRIYNVALSSRWIAAEHDNL
                     ASPSTFVIAGDEELIAP"
     gene            complement(16345..>16605)
                     /locus_tag="VMZ53_10290"
     CDS             complement(16345..>16605)
                     /locus_tag="VMZ53_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28893.1"
                     /translation="QGQVYVRPHEIDVARTQTPGSVPARVLRVTPLNAGLKIELHSEA
                     LGSNVRADIDWQRGSALALRDGDDVYVSLRRARAFNDEKVAA"
BASE COUNT         2906 a         5337 c         5472 g         2890 t
ORIGIN      
        1 ctgcctgttt gtcaccggcg atatcgggga ggcagactcc gtcggtcgat tcgtcgagga
       61 gctcgggcgg atcgcaccgg tctactacgt gctcggtaac cacgattatt accgagccag
      121 cattcacgcc gttcgcgaga atctcccagc ggggtggctg cctgccgcgg ggcccgtcgc
      181 gctgaccgag cacacggtgc tgctcggggt cgatggctgg ggtgacgcgc ggtgcggcga
      241 cctcgattcg aacgtgcagc tctccgattg gcagctgatc gaggacttcg agcgggtgcg
      301 ctacgaccgc aaggcgcgcg tcgcgctgct tcaggagctg ggcaccgccg aagcattcgt
      361 gcttggcgag ctgctcgcac gtgcacccgc gagccagcgg cttgtcgtgc tcacgcatgt
      421 gccgccgttt cccgaagcgt gcgtctacga tggtgcgcag tccgagccgg cctggctgcc
      481 gtggttcacg tgcatcgcga caggacagct gctcgccgaa tatgcgcgcg cgcatccgca
      541 gaagcagatc acggtgctct gcggtcactc gcatggcgcc ggtgtgtgcc ggccggagcc
      601 caatctcgaa gtgcgaaccg gtggctggcc gccgggcgtc gagggctacg gcaatccgat
      661 cgtgcaggcg acgctcgagc taccctgagg ttactcagct gtcgcggtct cgacgcgagg
      721 cagcgagcgc ttgccgagca gccgatagat gcagtcgcgt gcgcgcgtga tctcgatcga
      781 cgacgtctcg tcgccgcgca catcttccat gggccgcgtc tcgaggcgcg cgatcgcggc
      841 gttgaccgaa tcgacacacg tcagcgtccg gcatgcgcat gcacgaccgt ggagctcggc
      901 gctgaccttc tgccagctcg cgagatcgaa ccgcagcggc gcatcgagct ttggatcggc
      961 gtcggaatcg accgcgtaat tccacggcgg atcatcgagg cgcggtgggc aatagtggtt
     1021 cgtcgcttcg cacgtctcga gcgtggcgat gaagcggcgg cgcacggcga ggtctgcacg
     1081 cgcggcgttg acgggatccg gatcgcccgc cgattcgagc tcggtgatgc gcagctccca
     1141 cttcgcttcg gcggcgcgct cggcgaacaa cgccttctgg acctcgccct tgtcgtagct
     1201 cggcgcgggc tcaccgccgt cgtcctcggc gagggacgtg tgataggtga cgtcgtcgaa
     1261 gccagctccc gcaccgcgtg gcgaaccgcc tgatttgcat gcggccaatg ccagaaggag
     1321 tggcagcact ctcattgccg ctagggtata cctgcaactg ctcaagactc atggtttctc
     1381 atccatgaat cgctacagga cgccatgcgt ctacataggt atcaatgatc gacatcatcg
     1441 gaatcctcac ccgcacgtgg cttggcgttc atagccctgc gccgcagcaa ccggcgcagc
     1501 ctgttccggc ggtcgcgcgc gcgaagcaac acgtcatgac ggcgccgctc atcggcacgc
     1561 ccgcacccgc tgcggctgca acgggttctg cagcacctgt acccgcaaag acgccggcca
     1621 ccgcgcccgc gaagacgcct gcgcctgcga agacgccggc ggcaacaccc gcgaagggct
     1681 ccggcgcagc cgatgcaaag actccagcac ctgccgcaga tccgaagacg gttccggcaa
     1741 cggctccggc gaacgccgat gcggtcgtca cggaagtgca gaagttctac gcgggcatca
     1801 agcaggtcac cgcgcagttc cgtcagtcgg tcacgaacaa cacgttcgga agcacgaaga
     1861 ccagcgacgg tacggtctgg ctgatgaagc ccggcaagat gcgctgggac tacgtcgaga
     1921 agaagaagga caaggtccag gtcaagaaga gcttcatctc gaacggcacg tacctctacg
     1981 tcgtcgagca cgacaacaag caggtcgtga agaagaacct cgcgaacgac ctgatgccgg
     2041 tcgccgtcac attcctctac ggcaagggca acctctccgc tgagttcaac gcggcgatcg
     2101 acacgaccgg caagtacggc gcgaaggacg acatcgtcct caagatgtcg ccgaagaagc
     2161 cgagcgcgca gtacaaggcg ctgttcctcg tcgtggcgaa ggacaactac cgcgtgaagc
     2221 agtcggtgat cgtcgactcg tccgacaacg tgaaccagtt catgttcttc ggtcccgact
     2281 tcgagaagtc gatcaaagac tcgtggttcg agttcgacga gcgcagcgta aagaactacc
     2341 gcatcatcga tgccgatcag ccgcagggca gcggtgctgc gccggccgct ctgccgccgc
     2401 cggcgaagta atcacaacgt tgaatcgctg gtcgtccaag cccgagggcc acgcccggca
     2461 gtcggaccgt cggatcgtct acgaccaggt tcacgtcgcc gtcgccgtca acgttcacga
     2521 cgacgactac gttcgatgtt tagatctgct ggaccggctt accggccttc acccaaccga
     2581 cgatgccctg cggcatgagg aagacgttcg cgtaaccgag cttcttggcg cgcccggcgc
     2641 caacatggga agcgtgtcac gtctctttcg agcaatagaa cacgagcttc gtggtcttgt
     2701 cggcgggtag ctcgctcggg ccgtactgct cttcgtcggt gacgagcacc gcgccgggca
     2761 ctacgcccat ctgcttgcgc gtgccatcac cgttgcagtc gacgggcacg atctgctttg
     2821 cctcgagctg agccgcaact tcgtcgacgg tgagcgtcgc gaactgctct tcggccttcg
     2881 cggcagagtg atcggctttc ttgcagcccg ccgaagcggc tgcgacggcg agcacgacgc
     2941 caaacagaac gttgcgcatg cgcttaagat accctgaacg cgtggaaacg gttcgcgcaa
     3001 acggtctgca gtttgcctat ctgagcgaag ggacgggccc gctcgtgctg ctgctgcacg
     3061 ggtttccgga tacggcgcac acgtgggatc ggacgatcaa ggagctggcc gctgcggggt
     3121 ttcgcgcggt agcgccgttc atgcgcgggt atgcgccgac ggagattccg gcagacggcg
     3181 attacagcgt cgatacgctg ggccgcgacg cgctggcgct catcgaggcg ctcggcgaga
     3241 agaccgcgat cctcgtgggc catgactggg gcgcggcagc ggcgtacgcg gcggctgggc
     3301 tcggaccaga gcgcatctcg ctgctcgtga cggtcgcgat tccgcatccg aagtcgctca
     3361 agccgaatcc actcctgctg ctcaagcagc gccacttctt cgcgcttcgc ggcaagaaag
     3421 cgggcgcgcg cgtgcgcgag aaggacttcg cgtacgtcga cgagctgtgg cgacggtggt
     3481 ctccagcgtg gaaggacatt ccggcgagcg agaccgcagc cgtgaaggcg gcgttcgctg
     3541 atgcaggacg cgcggaggcg gcggcggcgt actactcgtt cgtcggattt cggccgccga
     3601 agtcgtacga cgcgaaagtg aagatgccgg cagtcgcgtt cgccggcgaa cacgacgtga
     3661 tttcgtcacg cgcgtacgag aaagcgcgcc actgctacga gaagtcgtac gaggtcgtca
     3721 tcgtgccggg cggtcacttc atgcaccgcg agcatcccga cgcgtttgtg cccgagctcg
     3781 tgcgcgtcgt gagcgatcac gctaagcggt gagctgctgc acgaggcccg cgaagttcgc
     3841 ttcgacgcgc tcctcgagga ctttgccgtt ctgcacggtc atgatgccga tgtacgtgaa
     3901 gctcacgcga cggttcgtcg ctttgatgcc ctggaagtca ccgcgctgtg tgccctcgaa
     3961 cgagcccgtg tgaataacgc gatcgcccgc gctgaagatc tgatcgtgca cgtggcggcc
     4021 atcggggaac gcggcgaaga acgcctgcga gaagccggcc cactcgtcgg gcgtcatcgc
     4081 ctgcccgccc atcacgaagc gcgcattcgg gagcatctgc gccttggcag cctcgaattt
     4141 ctggctgtcg atcagctcga acacgttgac gaggaacgcg cgatgatctg acggcggacc
     4201 ggcgagctgc tgcatgaggc ccgccgagtc gaactgccca cgatgctcga cgatgcgacc
     4261 atcgacgacg cgatcgacat ggattacttc catcgtgatc gaacggcccg tcgcggggat
     4321 gcccatgaag tcgcccttgt gcgttgcgcg caacgtgcac acgaccgtcg cgtactcgcc
     4381 cgcgacatga gccgcgacga tctcgtgctt cgtatcggga aatgcggccg accacatctg
     4441 gccgaactgt ctccagcctt cacgatcggc ggtcgtgcca ccgatatagg cgcgcgcgtt
     4501 cggcgcgacg agctcgagga cgccgggcca atcaccgcga tcgaaggcgg cgatcatttt
     4561 cagagaagga tgatttgcgt gagtgctttg catgagactc acgctacgga tctcggggca
     4621 acacctctat agggcgtatg ccctacgcgg acgtaagtcc cttggacatc gcgcaaatcg
     4681 ccgctgcggc acgcgtcgta acgccaagct tttcgaagat gtgctggacg tggtgtccgg
     4741 cggtcttcgt cgagatttcg agcgagctcg cgatctcttt attcgtgaac cccttcgcga
     4801 tcaggcgcag cacatcgatc tcgcgatccg tgaggccatg tgcgcgatcg actttcttga
     4861 cgacgtggcc tgcagcagcg agcactgcgc gcgcggcgtc gggacagagc gagccgccct
     4921 ccgccatctt cgtcatctcg gccgccgcgt cttcgcgagt catcgccttg cgatgtggac
     4981 ggtcttcgcc gagcgcgacg tacacgtcgg ccgctgcgag aattcgcgca gcgggtggac
     5041 acgcagccgc cgagagccgc cgatgatagc cggcgccgtc gaggcgttcg tgcgcgagac
     5101 atgagagctc ggcgatcgcc gccagtgcgg gtgcacgcgc gagcacacgc tcgccgatgt
     5161 acgtgtgcaa tcgaatcttc tcgcgctcga gatccgtgag tggctcggcc ttgtcccaga
     5221 tcccagacgt gatcgcgacg cgaccaatat cgtgcaagag cccagcgcgc cgcaggtctc
     5281 gctgcatcgc ctcgtcgagc cccgcggcct ttccagcggc agctgcgagc tcgctgacgg
     5341 ctgtcgagtg gccgcgcgtg tatttcgatt tgagatcggc gaacgcggcc attgtcgcga
     5401 gcgcttcgtc gatcgcatcg ccgttgactt ctttctgcgg tgccggctcg gcctcgagaa
     5461 atgccttcca gatcgatggc acgtcgatcg ctgatacgac ccggtcggag agcgcgacga
     5521 acttctcgac gatgtttgga tcgaggccct tcttcgagag ctgcttcacg cgcgagaccg
     5581 cgccttcggc cccaccgagc ttgaagccga tcacgacgtc gaacgcgacg tgaacgagac
     5641 gcatcgtcag cgcgatcgcg tcgcctttga gcttggccgg catgccgctg ccattccatc
     5701 gctcactgtg ctggacgagc gccgcacgca acgtagcgtc cataccgagg cgcgctgcaa
     5761 gcaagtcggc gacctcgcaa tgggcacgtg aaaccgccgg catcttcggc agcttgccga
     5821 gcgcgcgcgc catcgccatg gctccgccga cgaccccttt gccgcgccgc gcgctgcgca
     5881 gtaccgcagg cagcacttgc cgagcgtcgc cgtagtcgag cccataggtg tcgcgaccaa
     5941 actgcagctc gtcgccgaac agcgagaacg cgatgtcgtt gtcggcggtg caccccgaat
     6001 agcgcagcag cgcgaggtaa tacgcatcgc tctgctctcg cggcgtggcg ccgataacct
     6061 cgccgagttt cgcggcggcg attgctgcca tcagacccga ttcctccggc atagccgtac
     6121 cggcgtccgt ggcgagagag agcgtaccga cgagctcggc aaggcgcatc gcgggcatca
     6181 tgcacgacgg ggcacacgac gtgctactca ctcacgacga tgcttgtgcg aatcggcgcg
     6241 cgcacgaaca ccgaggacgt cgtcgacctc ctcgccgggt gccatgcgcg cattcggcag
     6301 tttctgacga tgtcgcaacg gctcgcgact gacgcggcat ctgacgacga cgtgcgcgac
     6361 accgcagcgc agatccatcg ctatttcacg tcggcgttcc tcgatcacgt cctcgacgag
     6421 gacgagctca tcacgccacg cctcgtcggg cgcagcgaca agctcgacgc gatcctcgct
     6481 gcgatgcatc gcgatcacgc gcagcacgca ggcttgatcc ggcggctcgt gtccgagtgc
     6541 gaaatcatcg agaaggaccc gcgcctggcc ggtgctctcg gcgccgagct gctcgacatc
     6601 gcgaagctgc tcgaggcaga gctcgtcccg catctcgagc tcgaggagcg cgagctgttt
     6661 cccgcgattc gcgggctgcc gcagagtgtg cgcgacgaaa tccgtgaagc gatgcggcag
     6721 cggcgggagc agcgctagag cagcgagtcg atgcgcgcaa ggatgtcgtg aacgtcctcg
     6781 atgctgatgg ccgagaacac gacggacttg ccgcgctcgc cgagccgatc ctgatacgtc
     6841 tgcacgagcg tcggtgacag cacgatcgcg acgatcgccg agtcgccatt cgccatcgcg
     6901 aacgcgacct cgtcctcgga ctcgagcggc atcacgcgat agccagcgtc ttcgaagagc
     6961 tgcgcgccga tgacgccttc gccgagcatt accgcgtgat cgcgacggcg gccgaggcgg
     7021 agtagatagc cgaggagcgc gtcgtcgaag ccgggcggct tgtcgacgat ctgcatgcct
     7081 gcaccgggcg tgcgtcccgt cgtttcggcg gcggtcggat cgatcaggaa cacgaccttc
     7141 gcgcgcacgc tccagttgcc ctgccccggc agctcgacct ggacgtcggt ctccgtgtgc
     7201 atcggcagct ggtgcgcgcc cttgatgaac aggccgcctg ccgagaggtt ctcgacgtag
     7261 tccgtcacga actgttcggc gttcgtgtac cgcacggcca gcctgacccc aaaccgcggg
     7321 tgcttgcgct tgtccatgcc gggcgtcgcc atgactgaat tatccgctgg atccggcagg
     7381 aagcgagaaa tcggtgcgat gctgcttcta tggccgagcg gccacttctc attttcgtca
     7441 gtgacattca tctgacggac acgctccacg cgaacgcggt cagcaaggcc gagcagtttg
     7501 gccggttctg ggatcgcatc caggtcgcgc gcggcaagcg gcctgccgag ctgtgcgtcg
     7561 tcggcgattt gttcgatctc gtgcggtcac cgtcgtggtt cgacggcagg caccggccgt
     7621 atcacggcgc atcgaacaac ggcgtcgtga agaacgtcga gcagattgtc gaggcgtgta
     7681 tcgcgcgcga gcaaggcttc ttcgacgcgc tgaaagcgaa gatcaagagc ggcgagctcg
     7741 tgctgcatta cgtagtcggc aatcacgatc gcttgttgct cacgtcgccg ggcgcgcaga
     7801 agaagatcgc cgatgcgctc ggcctgccga agctcgtgct gcatcgcgag ctcgcgtttc
     7861 ccgatcacgg cgtcctcgcg tatcacggca acgttggcga tccgatcaac gcgagccccg
     7921 aaggtgacgc tacgatcggc gatgcgatcg gcagcgagct gatcctgaag ttcccgcgca
     7981 agttgcgtgc gctcgtcggt gcagatcatc cgggcgttga agagatcgac gacatcgacg
     8041 acgtccggcc gatctacgcg gtacccgcgt gggtgcgcca gcagagtgca atccgcaagg
     8101 acttgctgcg gccgatcagc caggtgtgga gcgaggtcgt cgacgagttt ttggcgaacg
     8161 acttcgtgaa gcagtggctc aagacgcaga acaagctgtt ctcgctcgac ctcggcaaga
     8221 agctgcgcct tctcctcgag ctgtcacgca acaaggtgat ggcgcacggc tcggacgagc
     8281 gactctcgca gctgtatcga ttcttccagc acagcttcga cggcaagatg cagcaggtcg
     8341 ccgccgagga gttgctgaag cgaaagggcc tgcgctacgt cgtgaatggc cactcgcact
     8401 ttccgtcgat gcagccactc ggtcgcatcg gcgatgccgc gagcgtgtac ttcaacaccg
     8461 gcacgtggcg tgcggtgcat cagatcggcc atgacctcgg cggtcgcccg agcttcgtcc
     8521 agtacgacgc gatgacgtac ctcgtgttct ttcccaccga cgacaagctc ggccgcgact
     8581 acgagtggtg gaccggcgcg atggtcacgc gcgagcaggc actacacgcc tagcgcttgc
     8641 cggcctgcca ccaaccgtag agggtcgaca gcgtcgggga tgcgatcgga gagccctgcc
     8701 cttcgagcgc gcgcgtcagc tctttgccgt cgaaccagat gccgtgctcc ggacattgat
     8761 cgacggtgac cggaccgagc aggcagaccg tcatcatgac gtcgcagctc gggcatttcc
     8821 gcggcgcatc gctgccgccg gcgcgaatca gcttggagat ggtgcgcggc tggtccggcg
     8881 ccatgtgctg catcatctcc tccacttcct cggaccgtac gagcatcccg ttgcactgct
     8941 tgcaatgcag gcgcgttccg gagaggacga gctcgattcc acaggacggg cacatgctga
     9001 ccagcctact ggagattgct aaggtgcggg catgtcgtcg tcaccgcttc gtccccaccc
     9061 gcgtcatccc gggccaaagg ggcccgtcgt cctggtcgtc atggatggga tcggccgagg
     9121 cgccggcgac gaagccgatg ccgtctcgat cgcatcgacg ccgacactcg atcggctgtg
     9181 ggttcccggt gcgcgcgccg agctgaaggc acatggcaga gcggtcgggc tcccgaccga
     9241 agacgacatg ggcaacagcg aagtcggcca caacgcgctc ggcgccggca agatctacgc
     9301 acagggcgcc atgctcgtga accgcgcgat cgcgagcggc tcgatgtacg agggtgcgac
     9361 gtggcagcag atcatcgcgc gtggcgtgcg aggcggtgcc gtgcacttcc ttggtctgct
     9421 ctccgacggc aacgtgcact cgcacctcga tcacctcgaa gcgatgatgg gcacggccgc
     9481 gacggcggga tgcaagcggc tctacgtcca cgccctgctc gacgggcgtg atgtacctcc
     9541 cacgagcgcg ctggaatata tcgatcgcac ggagcggttt ctggctggat tgcgcgcttc
     9601 aggtgtcgac gcgcgaatcg tcagcggcgg cggacggatg aagctcacga tggatcgcta
     9661 cgaggccgat tggccgatgg tcgagcgcgg ctggcggacg catgtgctcg gcgatgggcg
     9721 gctgttctcg tctgcgaccg aggccgtgaa tacgtatcgc ggcgagtttc ccggcatcat
     9781 cgatcaggac ctgccggcgt tcgtcgtcgc gcccacgcag cccatgcagg atggcgacag
     9841 cgtcgtcatg ttcaacttcc gcggcgaccg tgcgatcgag atttcgcgcg cgttcgacga
     9901 cggcgaattc gcgccgttca atcgtgtccg gaaaccggac gtcttgtacg cgggcatgat
     9961 ggagtacgac ggcgatctgc acgtgccgcg tcactatctg gtcacgccgc cgcagatcga
    10021 gcgaccgatg ggcgagatcc tagcggccgc gcatgtttcg cagctcgcga tcagcgagac
    10081 gcagaagtac ggacacgtca cgtacttctg gaacggcaac cgcagcggca tgttcgatca
    10141 gggcacggag acgtacatcg agattccgag cgatcgcgtt ccgttcgagc agcggccatg
    10201 gatgaaggcg gcggagatca cggatcggct catcgccgag ctccgaaccg gcaagcatcg
    10261 gttcgcgcgc gtgaactacg cgaacggcga catggtcggt cacaccggcg cattcgacgc
    10321 cacggtgctc gcggtcgaag ccgtcgacct ccagctcgcg cgcctcgcgg ctgtcgtcca
    10381 ggagcttcaa ggaatcctgg tagttaccgc cgaccacggc aacgccgacg agatgttcca
    10441 gcgcgacaag aagggcgtgg tcatgcgtga caaagccggc caccccgtcg cgaagaccag
    10501 ccatacgctg aaccccgtgc cattcttgat cgacgatctc gcccgcggcg atcgctacga
    10561 gatcgaccca gagagagcga aagtcgcagg aatcgcgaac gtgaccgcaa cgtgcatcga
    10621 gctgctcggt tttgtcccgc cggatgatct agagtcttct atcttgaggt ttcgatgagt
    10681 acgaaagcgc tgatcggcat cctgctcgca ggcactgcag gcatcctgat cttcctgttc
    10741 gttcacctct cgggtggtga ttcgccgaag gtcgcgatcg gttcgaaaga ggctcaggcg
    10801 tgcgcgaagg gcggcgaaga ctgcctgccc gatgtgaact acgtcgacac gcaaggcacc
    10861 gcgtacacgt tcgcgtcgct gcacggcaag gtcgtcatcg tgaacttctg ggcgacgtgg
    10921 tgcggcccct gcaagaaaga gctgcccgag ctctcgcggc tctacaagaa gtacaaagcg
    10981 cagggcgtcg ttatgctcgg cgtcctgacg agcgacacgc ccgatgaaga cacgctgctc
    11041 aacttccaat cggactacga gatggagttt ccgatcgttc gccagacgtc ggacatcctc
    11101 gtctccttca actatccggg cgcgctaccg acgacgttcg tgttcgatcg ccacggcaag
    11161 cgcgtgggac cgccgcacgt cggtgcgatt cgcgccgatg agctcgacgc tcagctcgcg
    11221 cagctcgtcg cccagaagaa ctagaacgcg ccgctgacga cgaggcccgg cgccatgcca
    11281 ttggggcctt gcatcggtgc cggcacgacg gtgtaaccgt ggcgcgcgtt gaagcgatcc
    11341 gccgagtcgg cagcgtcgag cacgtcgtac caaacgcccc cgacgaatgc gatgccggca
    11401 atgccgagca gcggatacgc catgtcgttg aacgaattgc acgtcggctc gtgcggggcg
    11461 ttgatcacct cgcagcgcac cggctcggtc tgcgtctgca gcgtccagat cgcaaaaccg
    11521 gtggcgaccg cgcgcacgcc catgccccag gtcagatatt gaccggcgta catctcgccg
    11581 agtgatggag tgaccatcag catgccgatg cccgtgtaga ggacaggctt gttgaatgcg
    11641 tcgccttcgg gcgtcgtgat gaagcccgcg agcgtgatcg cgcccgagac gccagcggcc
    11701 gtgcccgaca tcacgcgcgc cgtgttgcgg ttgcgcggac ccgcggaggc ggtcgtcgag
    11761 atgagcaaga tcgcgagcag aacaatgcga cgcatcgacg gcattgtagc ggtctgccgg
    11821 gcctcgcgag gcacgatttc ccacacctcc taacgaccgc gaaccctgcg catctgccgg
    11881 gacaatctcc gccagaatcg cgcggtttca cacagcacta gggagccgtg accgtcacga
    11941 cgaggctcga ctgactgcta tccatcaccg cgcgaatggt gacgccaccc gtcgccaatg
    12001 cgtggacggt gccgcggtcg acggtcgcct tgctcgtatc agatgagatc cagatcgcct
    12061 tggtcgtgac gtccgcggtt gtcgcgtcgc tgtacgtagc ggtcgcggaa agctgaacat
    12121 tctgacctgc agccacgctc ggcgagctcg ggctgatcga gacctcgcga acgacgggca
    12181 cgaggaccgt aatgtcgatc gtcgagctga gatccgttgc atgtgcggtg attcgggtga
    12241 cgccgggttt gagagcgtcg agcgcgatct cgtgaccggc accggagagc gtcgcgaccg
    12301 tttcatcgct cgacgtccac gtcacgtcat ccgctggcag ctcgagcgcc tcgaacttgt
    12361 aggcagccgt gacggacgca tggccgttcc gctcgagcga cgtgtttgat gacttcagca
    12421 cgagcttgtc gggtgcctgc cccgcttcga acgtgcacgc agagagaaca agaccgaccg
    12481 cgatcgcaca ctgtttcatc ggcacggttc gatcgtacac ggcgcgaagt cgaaggagcg
    12541 tctcaccctg cgcagttgcg agtagttgcg cgtgtgcgca agtgggtttc acggcgtctg
    12601 cgtgatcgtc gcgccggtaa atggaaaccg cgacacgcgc tcccgcgtcg cggcacgtag
    12661 gtcgtcggcg tggcccgatg ccgagacgat gtccagctct gcgccgccat cgatgtcgcg
    12721 cgtcgtgatc gtcacgtcgt cggtgacgac ggggcagttc ttcatgcggc cgccgccctg
    12781 cccgtcgaat ccgccgtgac cttcgcgcgt gcgcttcgcg gcgaactcga cgatgtgatg
    12841 tgcgcgtttg cgagcgtcat cgagcttgtc ggccggcggg tggatcgtga agcgcacgcc
    12901 accgtcgatg tcgcggagct cggacgtcgc gccgtcgatc gcgagcgggc aatgccgcat
    12961 cttctcgtcc atctgatcag gcggcttctt cacgacgtcg ctaccagcgg ccggcggcgg
    13021 cgttggctgc gacttgtcgc ggccgcatcc gatcaacgca aggaggatga gcgtggtgag
    13081 cttcatgcgg gcagctcgag tgtaaacgtg gagccttctc caagcgtgag cgagccgtgc
    13141 attgcgaccg cgagctcgcg cgcgattgcg aggccgaggc cggttccgcg ccgctgtgga
    13201 tcgcgagcgg ccgcaccgcg gtggaagcgc tcgaacatgc gatcgcggtc ttctgccggg
    13261 ataccgggac cgctgtcctt cacgacgatg cggatgcgat tgtcggcgcg gttggcagcg
    13321 acgtcgatct ggacggctgc tccgccgtga cgaagcgcgt tgtcgacgag gttcaggatg
    13381 atccgctcga cggcatcctc gtcgccgatc ggcgcgagat cagacacgtc gacacgaatc
    13441 gaggcaccgc tctcgtcgac gcgcgccttg agcgtatcga tcacgtgttg caccaccggt
    13501 gcgagcgcca ctggctcggc agcgagtgga gcgcccttcc ccgcttcgat cgcttcgagc
    13561 tcgagcaagt cctcgacgag ctggccgata cgcagcgcgt tgcgatggat cgtcgttgcg
    13621 aattccttgc ccgtctcgtc gagcgagtcc gaggtcttca gcagctccgc atagccgcgg
    13681 atcgaggtga ccggtgtgcg gacttcgtgc gcgacgttcg cgaggaactc tcggcgatgc
    13741 tgctcggtcg cgcggagctc cgtggcggtg cgagcgagag cgcgcgagag gacgccgact
    13801 tcgtcatcag acggcgacgg ttgcacggca tcgtagttgc ccgcgccgat gcgttgcgcg
    13861 acgacggtcg catcgcggag ccggcgtgtg agtgggccgg cgacgagcgc cgcgagtccc
    13921 agtgcaagga gcaacgacgc gagggctgcc gcgaacagct gcgtgcgaag ctgcgacttc
    13981 gtctcttcga attcgccgat cggcacaccg aggcggacga ccgattcttg cggcgccggc
    14041 accgcgatgt agcgaaccgg ctgtgcttcg gccgacgaga accgcgtctc gcgtccgacc
    14101 gcgccggccc gcgcgtccgc gacttcctgc ggtgagcctt cgctgtccat gcgcggcgga
    14161 gtaccagcgg cggcctgcga ttcaccgacg gcgataccgc gcttgtcgat gatggtgacg
    14221 cgcgcgtcga cgacatcggc gaggcgatgc gcgagctgac cgggatgtgc tgcgcggctc
    14281 agccaggtcg cgaccgcgcg cgcttgcgtc tcgagccggt ggtcgagctg ctgttccatg
    14341 cggcgcgaga cgaggcgttc gacaacgacg agcgtcacgg cgctaaccac cagcgcgacg
    14401 agcgcgtgcg aggccaggag cttgaaccga aacgagatcg tcatcgcggt ccatggtaga
    14461 ggaagaagag atggcgacac gagtccttgt cgtggacgac gagcgcgacg tgtgccgcct
    14521 tctcacgtac agcctcgatc aggcgggctt cgcggcggcg agcgtgcaga ccgcagccga
    14581 agccctgatc gatgcggcgc ggcacgcacc ggcggtcgtc gtgctcgacg tcggattacc
    14641 ggatatgtcc ggtgtcgagg tctgcaggaa gctgcgcgcc gacaagcagc tcgccgacgt
    14701 cggcatcgtg atgctcaccg cgctcggctc gcgcgaagac cgcatcgtcg ggctcgaggc
    14761 cggtgccgac gactacgtcg tgaaaccgtt cgacgtccag gaagtcgtgc tgcgcgtgcg
    14821 cgcgctatcg cgccggatca gcgagcgcga gagcgcacgc aaaggctcgg caccgtcgac
    14881 cgcgcaggcg cttcgctccg ggacgctcac cgtcgatccg gtgacgcacg aagtccgcgg
    14941 cgatggcgag ctgctcagcg tgcgccctct cgagttcaag ctgctcgcga cgttgctcgg
    15001 cgaaccgggc cgcgtgtaca gccgaaggga gctgttgcac ctcgtctggg agatcgagca
    15061 cggcagcgag cgcaccgtcg atgttcacgt gcgcaggctc cgcttgaacc tcggcacgtg
    15121 ggccgatcag atcgagacag ttcccggctt cggctatcgc gcgaagcgct gacggtcagg
    15181 gtgcgatgag ctcttcgtcg ccggcgatca cgaacgtgct cgggctagcg aggttgtcgt
    15241 gctcggccgc aatccagcgc gagctgagcg cgacgttgta gatgcgaacc tcgtcgagcc
    15301 cgccccggta ttcgtagctc gtgaatccgc cgaggcacag cggacgtgtc gggttgatcg
    15361 aaggaccgag cgtgatcgcg gtggtcgttt gaattgctcc atcggcatac gccgccatca
    15421 agctcgcctt gcgatcgacg acgacgacga ggtgggacca cttccccggg gtcaccgcgc
    15481 cgagctccgc ggacaacgtg gtcgatgcat ccgcgatgta gccgcgccag tttccgtttc
    15541 caatctcgag atcgaagccc gctacgccgt cggtgaaccc gccgcgctcg aacaggacct
    15601 gaggcaccgt cggctgcgtt ggcacgaatg cccacgcctc gaacgagaac gagctcattc
    15661 ccggatcgag agagccgtcg ccgagtcccg tgcacatgcg attggtgccg tcgaacgtga
    15721 ctgccccacc ggccgcacct tgcgtcggcg cgggcggcgc gttgccgctg ctcagatcgt
    15781 tttggtgcga gctgcggtcg agctcgatgg agcccggcat cgagagatgc cacacaccga
    15841 cgaacggacc ggtccacggt gatgccttgg gctgcacgac cgatccgccg tacaacatgt
    15901 agaagtccgt cggcgacgcc gacaacgtca gctgcaccca cgcttcgatc gtgtcgccat
    15961 cgtggctgac gatctcgtat ggcagtggcg tcgtgccgtc gccagcggtg aacgtgatgt
    16021 cgcgaccgct cgggtccgcg tgggcttcga gtgcagggtc ggtcatccag acgagcacgg
    16081 ggaatgcggt gaggtcaccc gttccctgca ccgtgagtcg cttccgatag cgtgccgtcg
    16141 tgaccgctgc atcgccttcg gtgcggatgt cgtcgaggcc aaagagggca tcgcatcccg
    16201 tcagcgacag cacgagagcg agccacttca cgcaatcagc ttagcaaatc gctgcctaga
    16261 ggcgctggct catgtgacct tgtcggcgat cacggcgagc accgcgagcg cgagatacaa
    16321 gaggacgaag cgtttggccg acatctaggc tgccaccttc tcgtcgttga atgcgcgtgc
    16381 gcggcgcaac gagacataga cgtcgtcgcc atcgcgcaaa gcgagcgcgc tgccgcgttg
    16441 ccaatcgata tcggcgcgca cgttcgagcc gagcgcttcg gagtgcagct cgatcttgag
    16501 acctgcgttg agcggcgtca cgcgcaggac gcgagccggc accgagcctg gcgtttgcgt
    16561 gcgagcgacg tcgatctcgt ggggtcgaac gtagacctgg ccttg
//
LOCUS       DATLBZ010000196         4061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5407444, whole genome shotgun sequence.
ACCESSION   DATLBZ010000196 DATLBZ010000000
VERSION     DATLBZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5407444"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VMZ53_10295"
     CDS             <1..924
                     /locus_tag="VMZ53_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28894.1"
                     /translation="GRIVQAAEKLAAEVGDPVVVAFARSARGICELQWGDFTAAIESC
                     DKAIAAFREHGAGVVWEERTADVFGIWGLAWRGDWGEVARRCDALARAGEATGDRYAI
                     MHAAIGVAVCGPLALDRPQLARDRIAKVMTGWPRDSYDLIQVRELVGITTVDLYEGNG
                     AAALKLINEHWPRLEKSRMLSLEPLFGTLVDLRVRAALVAGEWSEAKSWAKKLERIPW
                     GAGVAAATRAALAQHNGDRSTAARELEHAETLARERGIELHSAAIAERRSRVLEGNDA
                     ILARDRAASITKAKEIQNPERTFRALVPWPT"
     gene            940..2511
                     /locus_tag="VMZ53_10300"
     CDS             940..2511
                     /locus_tag="VMZ53_10300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28895.1"
                     /translation="MTSPADTLIGTVVDGRFEIRDKIGEGGMGAVYRAWQRSTEREIA
                     MKLIAKHQLNEPMAIERFLREAKLASQLAHPNTISVYDFGKLADGRLFIAMEMVRGKS
                     LGRVLKTEGALAFDRVVRIGIQICDALAAAHALQIIHRDLKPDNVMVIDGTADLIKVL
                     DFGLAKRVNDKGLNATAAGVVVGTPRYIPPESVMTGATYAEGDLYALGVILAEMALGR
                     PLWRAAKSSSLADILQVQLKPAEHLREIPEPLRAIVASLVDPTPAMRPSASQLRAQLV
                     ALQSGSMPRNAQPSSPPPSASTPQAQAPQSPWARPTAHSTTIPDASPSFDSSAPTIRQ
                     RPASAAPPAPARPPAASRRSKDRSVATPTPRPRRWALFALGCLGVGIVVGAIAFALTR
                     HDAEKQVGASPVDAGRVVGTTAAIDASTPTRDAGVDAGDAATDTSIGSSAKPSTKVRT
                     PKAPPTTTAKKPCGDCLDNPWDDDLTFLKTEKSKPTPKGRGTGCQKSMLDARNPECVK
                     VYCANHPNEIACMTD"
     gene            complement(2526..3812)
                     /locus_tag="VMZ53_10305"
     CDS             complement(2526..3812)
                     /locus_tag="VMZ53_10305"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS28896.1"
                     /translation="MQIVCALGHVDPVGSVEQALSVRGHRVLTLESATLENAVGAELS
                     TADLVVVDADLATRFDLVSKIATNAPGIEFVLVGESAAAHPDVIDRLGRPLDVSRLLQ
                     IVDDLADILDGERHREPVELVAYETLFAGDSPHIRELLRRVRLVARSDAPVWVFGDDG
                     SGRSIIARAIHDRSARRQAPFLAFNSAAYSDEELCRRLFEGEQAAVNIARAGTVFIER
                     LALAGPATQRELVNYLESRKKDESGARLIVGIQREIATAGGSSLISTDLFYRLKVLEI
                     EIPSLKERARDLEQIVGRMLDRLGADNATSVSVETMRLLARYSFPGNVLELAHALTHA
                     FVLARGGAIEPQHLPLMIRKAAGEGELAASVEGELQALDVVAKRFERDYLLRVLRSVD
                     GNRGRAAEILGLSRKGLWGKLKSHGISDDDIEEEAT"
     gene            complement(3816..>4061)
                     /locus_tag="VMZ53_10310"
     CDS             complement(3816..>4061)
                     /locus_tag="VMZ53_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28897.1"
                     /translation="SSMTSIVGGAGAGPGRLGGETLFIATQQRPYRIFRFGNTAALQY
                     VPIVLTSWRTMGSMGNIELLAMDRSKSSVAAAHIGID"
BASE COUNT          732 a         1389 c         1324 g          616 t
ORIGIN      
        1 gggcgcatcg tgcaagccgc cgagaagctc gcggcagaag tcggcgatcc cgtcgtcgtc
       61 gcgttcgcgc gctcggctcg cggcatctgc gagctgcagt ggggtgactt cacggccgcg
      121 atcgagagct gcgacaaagc gatcgcagcc ttccgcgagc acggcgcggg cgttgtatgg
      181 gaggagcgaa ccgccgacgt gttcggcatc tgggggctcg cgtggcgcgg cgactggggc
      241 gaagtggccc gccgctgcga tgcgctcgca cgtgccggcg aagcgaccgg cgatcgctat
      301 gcgatcatgc acgccgcaat cggcgtcgcg gtatgcggcc cgctcgcgct cgaccgcccg
      361 cagctcgcgc gcgatcgcat cgcgaaggtc atgaccggct ggccgcgtga cagctacgac
      421 ctgatccagg tccgcgagct cgtcggcatc acgacggtcg atctctacga aggcaacggc
      481 gccgccgcgc tgaagctcat caacgagcat tggccgcgcc tcgagaaatc gcgaatgctc
      541 tcgctcgagc cgctgtttgg tacgctcgtc gacctgcggg tgcgcgccgc gctcgtcgcc
      601 ggcgaatgga gcgaggccaa gtcgtgggcg aagaagctcg agcgcatccc gtggggagcc
      661 ggcgtggccg ccgcgacgcg agccgctctc gcacagcaca acggcgatcg ctcgaccgcc
      721 gcgcgcgagc tcgagcacgc agaaacgctc gcacgcgaac gcggcatcga gctccacagt
      781 gcggccatcg cagaacgcag aagccgcgtg ctcgaaggca acgacgcgat cctcgcgcgc
      841 gaccgcgccg cgtcgatcac gaaggcgaag gaaatccaaa atcccgagcg cacattccgc
      901 gccctcgttc cctggcccac ctgatacgct ggacatccgg tgacgtcgcc tgcggacacg
      961 ctgatcggga ccgtcgtcga cggccggttc gagatccgcg acaagatcgg cgagggcggc
     1021 atgggtgcgg tgtaccgcgc gtggcagcgc tccaccgagc gcgagatcgc gatgaagctg
     1081 atcgcaaaac atcagctcaa cgaaccgatg gcgatcgagc gcttcctgcg cgaagcaaag
     1141 ctcgcgagcc agctcgcgca tcccaatacg atcagcgtct acgacttcgg caagctcgct
     1201 gacgggcgcc tcttcatcgc gatggaaatg gtgcgcggca agtcgctcgg ccgcgtgctg
     1261 aagaccgaag gcgcgctcgc gttcgaccgc gtcgtgcgca tcggcatcca gatctgcgat
     1321 gcgctcgccg ccgcgcacgc gctccagatc atccatcgag acctcaagcc cgacaacgtg
     1381 atggtgatcg acggcaccgc cgatctgatc aaggtcctcg acttcggcct tgccaagcgc
     1441 gtcaacgaca agggcctcaa tgcgaccgct gcaggcgtcg tcgtcggaac gccgcgctac
     1501 atcccgcccg aatctgtgat gaccggtgcc acgtacgccg aaggcgatct ctacgcgctc
     1561 ggagtgatcc tcgccgagat ggcgctcggc cgtccgctgt ggcgcgctgc gaagagctcg
     1621 agcctcgcag acatcctgca ggttcagctg aaaccggcgg agcatcttcg cgagattccc
     1681 gagccgctgc gcgcgatcgt cgcgagcctc gtggatccaa cgcccgcgat gcggccgtcg
     1741 gcgtcgcagc tccgcgcaca gctggtcgcg ctgcaaagcg gatcgatgcc gcggaacgcg
     1801 caaccctcgt cgccgccacc gagcgcgagc acaccgcaag cgcaagcgcc gcagtcaccg
     1861 tgggcgcgtc cgaccgcgca ctcgacgacg atccccgacg cttcgcccag cttcgacagc
     1921 agcgccccga caattcgcca acgaccggca tccgcagcgc caccggcgcc cgcgcgacct
     1981 cctgctgctt cgcggcgttc gaaagatcgt tcggttgcga cgccaacgcc acgtccgcga
     2041 cgatgggcgc tgttcgcact gggatgtctc ggcgtcggta tcgtcgtcgg tgcgattgca
     2101 ttcgcactga cgcggcacga tgccgagaag caggtcggcg cgtcgcccgt cgacgccgga
     2161 cgcgtcgtcg gcaccaccgc ggcaatcgac gcgagcacac cgacgcgcga tgccggcgtc
     2221 gatgcaggcg atgccgcgac ggatacgagc atcggctcga gcgccaagcc gtcgacgaaa
     2281 gtccggaccc caaaggcgcc gccgaccaca acggcgaaga agccgtgtgg agactgtctc
     2341 gataatccgt gggacgacga cctgacgttc ttgaagaccg aaaagtcgaa gccgacgccg
     2401 aaggggcgcg ggaccgggtg ccagaagagc atgctcgacg ccaggaatcc cgaatgcgtg
     2461 aaggtctact gcgccaatca cccgaacgag atcgcgtgta tgaccgacta gccgctcggc
     2521 ccgcgctatg tcgcttcttc ttcgatgtcg tcgtcggaga tgccgtgcga cttgagcttg
     2581 ccccagaggc ctttgcgcga cagcccgagg atctcggccg cacgaccgcg attgccgtcg
     2641 acgctgcgga gcacgcgcag cagatagtcg cgctcgaacc gcttcgccac gacgtcgagc
     2701 gcttgtagct caccctcgac cgatgcagcg agctcgcctt caccggcagc cttgcggatc
     2761 atgagcggca ggtgctgcgg ttcgatcgcg ccgccacgag ccagcacgaa cgcgtgtgtc
     2821 agcgcgtgcg cgagctcgag cacgttgccg gggaagctgt agcgcgccaa gaggcgcatc
     2881 gtctcgacgg acacgctcgt cgcgttgtcc gcaccgagtc ggtccagcat gcggccgacg
     2941 atctgctcga gatcgcgcgc gcgttccttc agcgaaggaa tctcgatctc gaggaccttc
     3001 agccgataga acagatccgt cgagatcagg ctgctgccgc ccgctgtcgc gatctcgcgc
     3061 tggatgccga cgatcaaccg agcgcctgat tcgtccttct tgcgcgactc gagatagttc
     3121 acgagctcgc gctgcgttgc tggcccagcg agagcgagcc gctcgatgaa caccgtgcct
     3181 gcgcgtgcga tgttgacggc agcctgttcc ccttcgaaga ggcggcggca cagctcctcg
     3241 tcggagtacg cggcactgtt gaacgcgaga aacggtgcct ggcgacgcgc gcttcgatcg
     3301 tgaatagcgc gggcgatgat cgagcgaccg gaaccgtcat cgccgaagac ccatacgggc
     3361 gcgtcgctgc gggccaccag gcgaacgcga cggagcagct cgcggatgtg cgggctgtcc
     3421 ccggcaaaca gcgtctcgta cgcgacgagc tcgacaggct cgcggtgccg ttcgccatcg
     3481 aggatgtccg cgaggtcgtc gacgatctgg agaagccgcg atacgtcgag cgggcgcccc
     3541 agtcgatcga tcacgtcggg gtgcgccgcc gccgactcgc cgacgagaac gaactcgata
     3601 cccggcgcgt tcgtcgcgat cttcgagacg agatcgaatc gcgtcgcgag gtcggcatcc
     3661 accacgacca gatccgcggt cgaaagctct gcgccgacgg cgttctcgag cgtcgccgac
     3721 tcgagcgtca gcacgcggtg cccacgcacc gacagcgcct gctcgacgct acccaccgga
     3781 tcgacgtggc cgagtgcaca gacgatctgc atcgctcaat caatcccgat gtgcgctgct
     3841 gccacgctcg atttcgatcg gtccatagcc agtaactcga tgttacccat gcttcccatg
     3901 gttcgccaag aggttaaaac aatggggaca tattgcaagg cggcagtgtt accaaaccgg
     3961 aagatccggt aaggtcgctg ctgggtggct atgaagagtg tctctcctcc gagccgaccg
     4021 ggtccggcgc cggccccgcc gacgatgctc gtcatcgacg a
//
LOCUS       DATLBZ010000197         8005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5408048, whole genome shotgun sequence.
ACCESSION   DATLBZ010000197 DATLBZ010000000
VERSION     DATLBZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5408048"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VMZ53_10315"
     CDS             <1..897
                     /locus_tag="VMZ53_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HUS28898.1"
                     /translation="QLYYTVDVDPPNLPHEFFAPTVTADDVSQVVSEQNPPVYVSSIG
                     YGRQVIFTFESDLAKSELEAALQFAYKGGAEISGSTSLTHQEVLSHTHTTAFILGGDA
                     GEAAQVAIGNYDQLKAFIGRGGNYSKDSPGAAIAYKLSYVRDHTPVQISYGSDYTQHT
                     CSRMTQKLRVVLEKIKVDSAGSDVGSNLEIFGSVTAIGSTRQVLVNWPEAQFKTIATG
                     ASFPSSGIVAETILPVKPAAGQSVRIETSLMEDDEFSNDSFGDALQTGAPFESGWRRT
                     LQIQRSSGTQAITLQVSLTPVP"
     gene            910..1800
                     /locus_tag="VMZ53_10320"
     CDS             910..1800
                     /locus_tag="VMZ53_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS28899.1"
                     /translation="MDGYYSSLFLGELDAAKRQQLESIPDLESRLASIYDAGRAAFQE
                     VVLDAPRLMRHLAAIVDDTGASLDALHVTDLYLACACAHGDPNAVGILEREFISTLDA
                     PLRSTGLDAHAVDDVRQRVREVLLVGSDHVPGIASYRGKGQLRSWLRAVAVRQAMMHF
                     RGKRETPVDDNALEGLAEIGADAQLAPWKRQYAAAFREAFEHSVKALSEHERMLLRQH
                     HLDQLSIDELATLHKVHRATAARQVASARAALLTRLRARMIEKLAISGGDLDSALRLA
                     RSQLDVSMHRLLGGKRQRKT"
     gene            complement(1785..3293)
                     /locus_tag="VMZ53_10325"
     CDS             complement(1785..3293)
                     /locus_tag="VMZ53_10325"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28900.1"
                     /translation="MRVVVSALLLAGCYQQSAVTPCTLTCSDSPCPSGLVCGSDFVCQ
                     APGMPLCSTTIMGDANDAGDAGAPPGLYIKAPNPDRDDLFGTSIALSSDGSWLAVGAP
                     GEASNAMLIDGSQTDNSLADAGAVFIYKWDGSSYVFATYVKSPSPDIGDHFGASISLS
                     ANGNVLAVGVPDEDGGGVDLTGNPTDDSVTNVGAVFIYARTSIAAAWAQTRYIKPPAA
                     VAQGHFGQSVSLSADGAVVAIGYPGNESAFRIKFTPSYIGYGGIPNGASAGDGVGTAV
                     AVAGNGDTLFGGAPFEDGPDTGFAGNPATNGATDAGAVYIETLNGAQNRYCKASNTGA
                     NDHFGAAITSDETAAVFAVGAPGEDSNATVTDGDQTNNASIDAGAVYVFGRTGAASWV
                     QTEYVKAPNAGGENFGSSVALTSVGTFLVVGAVGEGGRSGAAFTFEKTTSWQLRSTLK
                     APNHDPGDELGASVDVSDDRLVVAAGAPHEASTSSEPSDNSSPNAGAVYVFR"
     gene            complement(3305..4015)
                     /locus_tag="VMZ53_10330"
     CDS             complement(3305..4015)
                     /locus_tag="VMZ53_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS28901.1"
                     /translation="MRCALLHGFAGTPAAWDEVIDAWQLAEPPVAFALPGHGGGPVLP
                     TWDENISAIARVVDHFDAVVGYSLGARVALGLVAGGLVAHGVLIGVNPGIQDAERAAR
                     REFDAAWARMLRTEGVAAFHDAWTAQPLFATQKRVAPDELAVRRARRLSLDAEQLAQS
                     LEVMGLAGMPDYWPAVSAHRDRIALLAGADDAKYVALSASLPCAYFEQIPNSGHDPTL
                     ENPGELAAAIARAVEKLR"
     gene            complement(4025..4576)
                     /locus_tag="VMZ53_10335"
     CDS             complement(4025..4576)
                     /locus_tag="VMZ53_10335"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HUS28902.1"
                     /translation="MIAGIRQLGYDTTDPNFTETAARAAKGFHELIHDQKQVKPAIEA
                     LLAKTFPAKYTEMVISKHNTAFGVCPHHLLPVVYRISLAYIPTEKVLGLSKLTRLARL
                     IARGPRLQEDMTHELADILHVQLQSQGSAVYIEGLHMCMAARGVGAHEARLVTSGVRG
                     VFLELATREEFIKLVLAPQTTVL"
     gene            complement(4462..5688)
                     /gene="mutY"
                     /locus_tag="VMZ53_10340"
     CDS             complement(4462..5688)
                     /gene="mutY"
                     /locus_tag="VMZ53_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HUS28903.1"
                     /translation="MNAAEVAAAVVAHYSRDRRDLPWRRTRDPYAIWVSEIMLQQTRV
                     QTVIPYWERWMSRFPTVGALAQAPLDDVLAAWAGLGYYSRARNLHAGAQTVVTKLGGA
                     LPSRASELRAVPGIGPYTAGAIASIAYGERAPLVDGNVARVLARVYGIEEDIKSSGGQ
                     KLLWSRAGELMNALPAGHAPGDLNQGLMELGATTCSPTSPRCLVCPLAKLCVAAKTGR
                     QDELPVVAARKKESELPILARAALWIERDGVLLLARRTPGGLFGGLWELPQGESALEI
                     ARALGVTHLDDKAVAHHEQTLSHRRLRIAVFCAAVPRTLVAPALPGYDSLARVDLSRA
                     RELGVAAATTAILTKYEDTPWSSIPKRSLSSPRATKKSSRASVSSATTPPTPTSRKPR
                     RAQPKASTSSSTIRSR"
     gene            complement(5704..6417)
                     /locus_tag="VMZ53_10345"
     CDS             complement(5704..6417)
                     /locus_tag="VMZ53_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28904.1"
                     /translation="MGGVASAQPGPPPQPPPPPVDTTTTTTTTTTEQTPVVTNTTPTP
                     VVVASPTPQPQPVAESAGPRPDGMALGLGVGYQLPTSLETPNITSFRLRLAGGLTLEP
                     AFAVSNESETAEAAGVEATNKHTVFSLFAMGRLPLISRGKADFEGLARLGFTNDKDNP
                     DGDFNTTTTNRFGVGWGIAVGYWFSPHWQLSFNVTNDLIDFTSRKTQAGPGMTTKQSS
                     TEIGLIFAPSVFMMLHLYN"
     gene            complement(6786..7442)
                     /locus_tag="VMZ53_10350"
     CDS             complement(6786..7442)
                     /locus_tag="VMZ53_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28905.1"
                     /translation="MKRAMTLIALGGCVGDGTPDWQLDYDRIVAVRAEPPALPPGVVG
                     RLDVLVAHVGAPTTIEAPQTMSAPGSPLFTAVHYNIDHYEIDGPDEAQLVAARAELGL
                     PDGALVPLEVELRVSGPLYAKKLVLLGAAATNPVLPAVMIDGAVAGSTIDVAAHRDTP
                     LLIEATTAHWFTSCGELVGEREASARLVTGGACEGELVVVVREGENGVAWGVWPIRVR
                     "
     gene            complement(7439..>8005)
                     /locus_tag="VMZ53_10355"
     CDS             complement(7439..>8005)
                     /locus_tag="VMZ53_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28906.1"
                     /translation="RTRLVARDAFGNFGNSGTGTSQGVELGSFFLRERWAAYVGASTS
                     SSVRRDYPRGREHAADFDQPVRIDSIITYGKKRWRIAARFSLSSGLPYTPVIETTYDA
                     DADSYVPSYGFPNSERLPWMHSLDIRADMRLGRSWSAFAELRNAYAASTALGYEYSFD
                     YKQRLDVTLPILPFVGLRADIPVRGARP"
BASE COUNT         1390 a         2643 c         2604 g         1368 t
ORIGIN      
        1 cagctctact acacggtgga tgtggatccg ccgaatctgc cgcacgagtt cttcgcgccg
       61 accgtgacgg ccgacgatgt ctcgcaagtc gtgtccgagc agaacccgcc ggtctacgtg
      121 tcctcgatcg gctatggccg gcaggtcatc ttcacgttcg aatcggatct cgcgaagtcg
      181 gagctcgagg ccgcgttgca gttcgcgtac aagggcggtg cggagatcag cggaagcacg
      241 tcgctcacgc accaggaggt gctgtcgcat acgcacacga ccgctttcat cctcggcgga
      301 gatgctggtg aagctgcgca ggtggcgatc ggcaactacg atcagctgaa ggcgttcatc
      361 ggccgcggag gcaactactc gaaggattcg ccaggtgcgg cgatcgcata caagctctcg
      421 tacgttcgcg accacacacc tgtccagatc tcctacggat cggactacac gcagcacacg
      481 tgctcgcgca tgactcagaa gctgcgcgtg gtgctcgaga agatcaaagt cgacagcgcg
      541 ggctccgacg tcggcagcaa cctcgagatc ttcggctcgg tgactgcgat cggcagcacg
      601 cgtcaagtgc tcgtgaactg gccggaagct caattcaaga cgatcgcgac cggcgcgagc
      661 tttccgtcga gcggcatcgt cgccgagacg attctcccgg tgaagccggc cgctggtcag
      721 tcggtgcgca tcgaaacgtc gctcatggag gacgacgaat tctcgaacga ttcgttcggc
      781 gacgcactgc agaccggtgc gcccttcgaa tcgggctggc gtcgcacgct gcaaatccag
      841 cgctcgagcg gtacgcaggc catcacgctg caggtctcgc ttacgcccgt tccctgatac
      901 gctgaagcag tggacgggta ctactcgtcg ctgttcctgg gcgagctcga cgcagcgaag
      961 cgtcagcagc tcgagtccat tcctgatctc gaatcgcggc tcgcgtcgat ctacgatgcg
     1021 ggccgtgcgg cgtttcagga ggtcgtcctc gacgcgccga ggttgatgcg ccatctcgcg
     1081 gcgatcgtcg acgacaccgg cgcttccctc gacgcgctgc acgtcacgga cctctatctt
     1141 gcgtgtgcgt gcgcgcatgg cgacccgaat gcggtcggca tcctcgagcg agaattcatc
     1201 tcgaccctcg atgcgccgct gcggtcgacg ggcctcgatg ctcatgcggt cgacgacgtt
     1261 cgccagcgcg tgcgcgaggt gctgctcgtc ggcagcgatc atgttccggg tattgcgagc
     1321 tatcgcggca aaggccagct gcgcagctgg ttgcgtgccg tcgcggtgcg ccaggcgatg
     1381 atgcacttcc gcggcaagcg cgagactccg gtcgacgaca acgcgctcga gggcctcgcc
     1441 gagatcggcg cggatgcaca gctcgcgccg tggaaacggc agtacgcagc tgcatttcgc
     1501 gaggcattcg agcactcggt gaaagcgttg tcggagcacg agcgaatgct gctgcgccag
     1561 catcatctcg atcagctctc gatcgacgag cttgccacgt tgcacaaggt gcatcgcgcg
     1621 acggcagctc gtcaggtcgc gtcggctcgc gctgcgctcc tcacgcgcct gcgagcgcgc
     1681 atgatcgaga agctcgcgat ctccggcggt gatctcgata gcgcgctacg cctcgcccgc
     1741 agccagctcg acgtcagcat gcatcgattg ctcggcggca agcgtcagcg gaagacgtag
     1801 acggcgcccg cattcggcga cgagttgtcg ctcggctcgc tcgacgtgct cgcttcgtgc
     1861 ggggcacctg cagcgacgac gagtcggtcg tcggagacat ccacgcttgc accgagctcg
     1921 tcacctggat cgtggtttgg tgccttcagt gtcgagcgca gctgccaact cgtcgtcttt
     1981 tcgaacgtga acgcggcgcc cgatctgccg ccttcaccga cggcgccaac gacgaggaac
     2041 gtgccgacgc tcgtcagcgc cacgctggaa ccaaagttct cgccgccggc attcggcgcc
     2101 ttgacatact cggtctgaac ccacgatgcg gcacccgttc tcccgaacac gtacacggcg
     2161 cctgcgtcga tcgacgcgtt attcgtctgg tcgccgtctg tgacggtggc gttgctgtct
     2221 tcacctggag cgccgacggc aaacaccgcg gcggtttcgt cgctcgtgat ggccgcgccg
     2281 aagtgatcgt tggcaccggt gttcgacgct ttgcagtagc gattctgcgc accgttaagg
     2341 gtctcgatgt acacggctcc ggcatccgtc gcgccgttcg tcgctgggtt gccggcgaag
     2401 cccgtgtcag ggccatcctc gaatggcgca ccaccaaaca gcgtgtcacc gttgccggcg
     2461 accgccaccg cggttccgac accgtcgcca gcggaggcac cgttgggaat gccgccgtag
     2521 ccgatatacg aaggtgtgaa cttgatgcgg aacgcgctct cgttcccggg atagccgatc
     2581 gcgacgacgg caccgtctgc cgagagcgac acgctctgac cgaagtggcc ttgcgcgact
     2641 gcggcggggg gcttgatgta gcgcgtctgt gcccacgcgg cggcgatgct cgtgcgtgcg
     2701 tagatgaaga cggcgccgac gttcgtgaca ctgtcgtcgg tcggattgcc cgtgagatcc
     2761 acgccaccgc catcctcgtc aggcacgccg accgcaagga cattgccgtt ggcggagagc
     2821 gaaatgctcg cgccgaaatg atcgccgata tcgggtgatg ggcttttcac gtaggtcgca
     2881 aacacgtagg acgagccgtc ccacttgtag atgaagacag caccggcgtc ggcgaggctg
     2941 ttgtctgttt gcgagccgtc gatcaacatc gcgttgctcg cctcaccagg cgcgccgacc
     3001 gcgagccagc ttccgtcgga cgagagcgca atgctcgttc caaacaggtc gtcgcgatcg
     3061 ggatttggcg ctttgatgta cagccccggt ggcgcgccgg cgtctccggc atcgtttgcg
     3121 tcgcccatga tcgtcgtcga gcacagaggc atgcccggcg cctggcagac gaagtcgctg
     3181 ccgcacacga ggccgctcgg acacggcgag tcgctgcacg tcagcgtgca cggcgtcacc
     3241 gccgactgct ggtagcaacc ggccagcagc aatgcactga cgacgacccg cacgagaagc
     3301 gatcttaacg cagcttctcg acggcgcgtg cgattgctgc ggcgagctcg ccgggattct
     3361 cgagggtcgg atcgtggccg ctattcggga tctgctcgaa gtacgcacat ggcaacgagg
     3421 cggaaagtgc gacgtacttg gcatcatctg cgcccgcaag taacgcgatg cgatcgcgat
     3481 gcgccgacac cgccggccag taatccggca ttcccgcgag gcccatgacc tcgaggctct
     3541 gcgcgagctg ctcggcatcg agcgagagac gccgcgcgcg tcgcacggcc agctcgtcag
     3601 gcgcaacgcg cttctgcgtt gcgaacagtg gctgcgcggt ccacgcgtcg tgaaatgcag
     3661 caacgccttc ggtgcgaagc atgcgtgccc atgcagcgtc gaactcgcgg cgtgcagcgc
     3721 gctcggcatc ttgaatgccc gggttcacgc caatgagcac cccgtgcgcg acgagaccac
     3781 ccgcgacaag accaagcgcg acccgtgcgc cgagcgagta gccgacgacg gcatcgaagt
     3841 gatcgaccac gcgcgcgatc gcggagatgt tctcgtccca ggtgggcagc acgggcccac
     3901 cgccgtggcc gggaagcgcg aatgcgacgg gaggctcggc gagctgccac gcgtcgatga
     3961 cttcgtccca cgcggcgggc gttccggcga agccgtggag cagcgcgcag cgcatgaccg
     4021 aagtctagag gacggtcgtc tgcggggcga gcacgagctt gatgaactct tcgcgcgttg
     4081 cgagctcgag gaacacgcca cgcacaccgc tcgtcacgag gcgtgcttca tgcgcaccga
     4141 cgccgcgcgc cgccatgcac atgtgcagac cttcgatgta gaccgcggag ccttggctct
     4201 gcagctgcac gtgcaggatg tcggcgagct cgtgcgtcat gtcctcttgc aggcgagggc
     4261 cacgtgcgat gaggcgcgcg agacgcgtga gcttcgagag gccgagcacc ttctccgtcg
     4321 ggatgtacgc gagcgagatg cgatagacga cgggcaacag gtgatgcggg cacacaccga
     4381 acgcggtgtt gtgcttgctg atcaccatct cggtgtactt ggccgggaac gtctttgcga
     4441 gcagcgcctc gatggcgggc ttcacctgct tctgatcgtg gatgagctcg tggaagcctt
     4501 tggctgcgcg cgccgcggtt tccgtgaagt tggggtcggt ggtgtcgtag ccgagctgac
     4561 ggatgcccgc gatgattttt tcgtagccct cggtgaagag agcgagcgct ttgggatcga
     4621 gctccatggt gtgtcctcgt acttcgtcag gatcgcggtc gtagccgcgg cgacgcccag
     4681 ctcgcgagct cgcgagagat cgacgcgcgc cagagagtcg tagcccggca gcgcgggcgc
     4741 gacaagcgtt cggggcactg ctgcgcagaa cacagcgatg cgcaggcggc gatggctgag
     4801 cgtctgctcg tgatgcgcga ccgccttgtc gtcgaggtgt gtaacgccaa gcgcgcgggc
     4861 gatctctaat gcgctctcgc cctgcggcag ctcccacagc ccgccgaaga gaccgccggg
     4921 cgtacgtcgc gcgaggagca gcacgccatc gcgttcgatc cacaacgcgg cgcgcgcgag
     4981 gatcggcagc tcgctctcct tcttgcgcgc agcgaccacc ggcagctcgt cctggcgccc
     5041 tgtcttcgcc gcgacgcaca gcttcgcgag agggcagacg agacagcgcg gtgacgtagg
     5101 cgagcatgtc gtcgcgccga gctccatgag gccctgattc aaatcgcccg gtgcgtggcc
     5161 tgcaggcagc gcattcatca gctcgccggc gcgcgaccac agcagcttct gcccgccgct
     5221 cgacttgatg tcctcctcga tcccgtacac gcgagccagc acgcgcgcga cgttgccatc
     5281 gacgagcggc gctctctcgc catacgcgat cgacgcgatc gcacccgccg tatacggccc
     5341 gatgccaggc accgctcgca gctccgacgc acgcgagggc agggcgcctc cgagcttggt
     5401 gacgaccgtc tgcgcacccg cgtgcaagtt gcgcgcgcgc gagtagtagc caagcccggc
     5461 ccacgccgct agcacatcgt cgagcggagc ctgcgcgagc gcgccgacag tcgggaaccg
     5521 gctcatccag cgctcccaat acggaatcac cgtctggacg cgcgtctgct gcaacatgat
     5581 ctcgctgacc cagatcgcat acggatcgcg agtccgtcgc caggggaggt cgcggcggtc
     5641 acgcgaataa tgcgcgacga cagcggctgc gacctcagcg gcattcaccc gccggtttgt
     5701 acctcagttg tagaggtgca acatcatgaa gacgctcggc gcgaagatca ggccaatctc
     5761 cgtcgagctt tgcttcgtcg tcatgcccgg acctgcttgc gtcttgcgcg acgtgaagtc
     5821 gataagatcg ttcgtcacgt tgaacgacag ctgccagtgc ggcgagaacc agtagccgac
     5881 cgcgataccc cagccgacgc cgaaccgatt cgtcgtcgtc gtattgaagt cgccgtcggg
     5941 gttgtccttg tcgttcgtga acccgagccg tgcgagacct tcgaagtccg ccttgccgcg
     6001 cgagatgagc ggcagacgtc ccatcgcgaa cagcgagaac acggtgtgct tgttcgtcgc
     6061 ttccacgccg gcggcttccg cggtctccga ttcgttcgac acggcgaacg cgggctcgag
     6121 cgtcagtcca cccgcaagtc gcaggcggaa gctcgtgatg ttcggcgtct cgagcgacgt
     6181 cggcagctga tagccaacgc cgagaccgag cgccatgccg tccggccgcg gacccgccga
     6241 ttccgcaaca ggctgcggct gcggcgtcgg cgacgccacc accacaggcg taggcgtcgt
     6301 gttcgtgaca accggcgtct gttcggtcgt tgtcgtcgtg gtcgtcgtcg tcgtgtcgac
     6361 gggcggcggc ggtggttgcg gcggcggccc tggctgtgcg gaggcaactc ctcccatggc
     6421 caaggtcaaa acgagcacca gggatttaga cctcatcgca ttcctccgag tcgtggcgtg
     6481 gattgggagc acatgtcatc gcagtcatcg atcaacttcg agcaggtcgc cgagttctca
     6541 taaacgcatt gcaaacactc gtccgtgatc gtcggaagct gcgggacgag catgtcgacg
     6601 cattccttga ccgccgtcga accgatctcg acgcagttgc agtacgcggt cgcaacgtcg
     6661 tgcgctcgct gctcgggtga cacggtacac gccgtcaccg ccagcacgag cgccaagacc
     6721 atcgacatcc ccgagagtcg catcgcgaaa attgtcccgc gccgatcgcg cgtgtccaaa
     6781 ttttcctaac gcacgcggat cggccagacg ccccacgcga cgccgttctc gccctcgcgc
     6841 acgaccacga cgagctcgcc ctcgcacgcg ccgccggtca caagtctcgc cgacgcctcg
     6901 cgttcgccga caagctcgcc acacgacgtg aaccaatgcg ctgtggtcgc ttcgataagt
     6961 agcggcgtgt cgcgatgcgc cgctacgtcg atcgtcgatc cagccacagc gccatcgatc
     7021 atcaccgccg gcaacacagg attggtcgcg gctgcaccga ggaggacgag cttcttggcg
     7081 tatagaggcc cgctgacgcg cagctcgacc tcgagcggca cgagcgcacc gtcgggaagt
     7141 ccgagctccg cgcgcgccgc gacaagctgc gcctcgtcgg gcccatcgat ctcgtagtga
     7201 tcgatgttgt agtgcacggc cgtgaagagt ggcgaccccg gcgccgacat cgtctgcgga
     7261 gcttcgatgg tcgtgggcgc gcccacgtgg gcgaccagaa cgtcgaggcg accgacgacg
     7321 ccgggcggaa gcgcaggcgg ctccgcgcgc actgcaacga tgcgatcgta gtcgagctgc
     7381 cagtcgggcg tgccgtcgcc gacgcagccg ccaagcgcga tcaacgtcat cgcacgcttc
     7441 atggtctggc gcctcgaacc ggaatgtccg cccgaagtcc gacaaacggc aggatcggca
     7501 gcgtgacatc gagccgctgc ttgtagtcaa acgaatactc gtagccgagc gccgtgctcg
     7561 cggcgtacgc gttgcgcagc tcggcaaacg cggaccagct cctgccgagt ctcatgtcag
     7621 cgcgaatatc gaggctgtgc atccacggta agcgctcgct gttcggaaac ccgtacgaag
     7681 gcacgtagct gtctgcgtcg gcgtcgtacg tcgtctcgat gaccggcgta tacggaaggc
     7741 ccgacgacag ggagaaacgc gcagcgatcc gccagcgctt cttcccgtac gtgatgatcg
     7801 aatcgatccg cactggctga tcgaaatcgg cggcgtgctc gcgcccacgt gggtaatccc
     7861 gccgcaccga cgacgacgtc gaggcgccca cgtacgcggc ccaccgctcg cgcagaaaga
     7921 agctgccgag ctcgacgccc tgcgacgttc cggtgccgct attgccgaag ttgccgaacg
     7981 cgtcacgcgc gacgagccgc gtacg
//
LOCUS       DATLBZ010000198         2556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8292973, whole genome shotgun sequence.
ACCESSION   DATLBZ010000198 DATLBZ010000000
VERSION     DATLBZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8292973"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VMZ53_10360"
     CDS             complement(<1..609)
                     /locus_tag="VMZ53_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUS28907.1"
                     /translation="MLRVFHVSDLHFGRPTVPEQIEAIEQIIQEGRFDVVAISGDLSQ
                     RAEAGEFQRAQAFIRDANKVSKTIVVPGNHDVGWWKAPMGVGDDLKRYEKYRQYVSED
                     LEPVLRVPGATFVGLNSARGVTRHTLTWRVRDISIIGAIGRSQIERAREEFEGSPEDD
                     ARVIVMHHNPVKGVLSGRHGLKDTRKVLGAFANMAVDLVLCGH"
     gene            complement(625..1641)
                     /locus_tag="VMZ53_10365"
     CDS             complement(625..1641)
                     /locus_tag="VMZ53_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HUS28908.1"
                     /translation="MAVPCTLIPGDGIGPDITDATVRVLEAAGAKFDWDTRLAGMAAV
                     AKFDDPMPDETIESIEKTGLALKGPLETPVGGGYRSVNVALRRTFDLYANVRPARSVV
                     SGGRFDNVNIVLIRENTEGLYSGIEHFISIGSDPRAAAESTAVVTRFGSERIIRYAFE
                     YAVAHGRKKVTLVHKANILKMTGGLFREVGATIAKEYAGRVEYEERIVDAMAMQLVIK
                     PEVFDVVVTTNLFGDILSDLISGLVGGLGLAPGANLGTNAAIFEAVHGTAPDIAGKGV
                     ANPGALIYAACLMLEHLNDHARGARIRDAMESVIREQKTVTRDLGGSATTAQFTDAII
                     ARLA"
     gene            complement(1655..2365)
                     /locus_tag="VMZ53_10370"
     CDS             complement(1655..2365)
                     /locus_tag="VMZ53_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28909.1"
                     /translation="MSANHFSFTGFAALAIVASCARASATAAPAAMSASAPAASVQKP
                     AAPQQGSMRGALAPLASEPLVVLPVQTLVMTVPDWSDKVGDPRAFLSGLDDEIVFAVR
                     ERSLRGKWAFAPDLARSAKRNPTYAVDPYTIALDPIKPVERDAEKIVGEPLAGQLRAF
                     AGLFNARYALVPVELRLAPDASGGARATIHAVVIDTRSAKLTWKGDVSGDVVHSFSPA
                     VAAGIAGRVADLFITPAR"
BASE COUNT          450 a          830 c          841 g          435 t
ORIGIN      
        1 gtgtccgcac agcacgaggt ccacggccat gttcgcgaat gcgccgagca ctttcctcgt
       61 atccttgagt ccgtgtcggc ccgacagcac gcccttcacg ggattgtgat gcatcacgat
      121 cacgcgtgcg tcatcttccg gcgagccttc gaactcttcg cgcgcgcgct cgatctgcga
      181 tcggccaatt gcgccgatga tcgagatgtc tcgcacgcgc cacgtgagcg tgtgtcgtgt
      241 gacaccgcgc gcggagttga gcccgacgaa cgtcgcgcca ggcacacgca gcacaggctc
      301 gagatcctcc gagacgtact gccgatactt ctcgtacctc ttgaggtcgt cgcccacgcc
      361 catcggcgcc ttccaccacc cgacgtcgtg gttgccaggc accacgatgg tcttgctgac
      421 tttgttggcg tcgcgaatga acgcttgcgc ccgctggaac tcgccagcct cggcgcgctg
      481 cgataggtcg cccgagatgg cgactacatc gaagcgtcct tcctggatga tttgctcgat
      541 cgcctcgatc tgctcgggca ccgtcgggcg cccgaagtgc agatcggaga cgtggaacac
      601 cctcagcatg gactcgagcg ttgcctacgc gagacgagcg atgatcgcgt cggtgaactg
      661 cgcggtcgtg gcgctgccgc cgaggtcgcg tgtgacggtc ttctgttcgc gaatgacgct
      721 ctccatcgcg tcgcgaatgc gcgcgccgcg cgcgtgatcg ttcagatgct cgagcatgag
      781 gcacgcggcg tagatgagcg caccgggatt cgcgacgccc ttccctgcaa tgtccggcgc
      841 cgtgccgtgc accgcctcga agatcgcggc gttcgtgccg agattcgcgc caggcgcgag
      901 gccgagcccg ccgacgagcc ccgagatgag atcggagaga atgtcgccaa acaggttcgt
      961 cgtgacgacg acatcgaaca cctccggctt gatcacgagc tgcatcgcca tcgcgtcgac
     1021 gatccgctcc tcgtactcga cgcgcccggc gtactccttc gcaatggtcg cgccgacttc
     1081 gcgaaagagc ccgcccgtca tcttgaggat gttcgccttg tgcacgagcg tgactttctt
     1141 ccgcccatgc gcgacggcgt actcgaacgc ataacgaatg atgcgctcgg agccgaatcg
     1201 cgtgacgacg gccgtcgact ccgcggcggc gcgcggatcc gaaccgatcg agatgaagtg
     1261 ctcgatgccc gagtagagcc cctcggtgtt ctcgcggatg agcacaatgt tcacgttgtc
     1321 gaaccggccg cccgaaacga ccgagcgagc gggacgcacg ttcgcgtaca gatcgaacgt
     1381 ccggcgcagc gcgacgttca ccgatcgata gcctccgccg acgggagtct cgagcgggcc
     1441 cttgagcgcg aggccagttt tttcgatcga ctcgatggtc tcgtcaggca tcgggtcgtc
     1501 gaacttcgcg accgcggcca tgccggcgag acgcgtgtcc caatcgaact tcgcgccggc
     1561 ggcttcgaga acgcggacgg tggcgtcggt gatgtccgga ccgatgccat cgccggggat
     1621 caacgtgcac ggaacagcca tgtgtggagt cggttcagcg agcaggagta atgaagagat
     1681 cggcgacccg gccggcaatg ccggcggcga ccgcgggtga gaacgagtga acgacgtcgc
     1741 ccgagacgtc gcccttccag gtgagcttcg cggagcgcgt gtcgatcacg accgcgtgaa
     1801 tcgtggcgcg cgcaccgccg ctcgcgtccg gcgcgagccg cagctcgaca ggcacgagcg
     1861 cataacgcgc gttgaagagg ccagcaaacg cacgcagctg tcccgcgagc ggctcgccca
     1921 caatcttctc ggcatcgcgc tcgaccggct tgatcggatc gagcgcgatc gtgtatgggt
     1981 cgaccgcgta cgtgggattc cgtttcgccg atcgcgcgag gtctggagcg aacgcccact
     2041 ttccgcggag cgaacgctcg cgtaccgcga acacgatctc gtcatccagg cccgagagga
     2101 acgcgcgggg atcgccgacc ttgtcactcc aatcgggaac ggtcatgacc agcgtctgca
     2161 ctggtaggac caccagcggc tccgacgcga gcggtgcgag cgcgccgcgc atggagcctt
     2221 gctgcggtgc agccggcttt tgcacggatg ccgccggtgc ggacgcggac atcgctgcgg
     2281 gcgcagccgt cgcggacgcg cgtgcacagc tcgcgacgat ggcgagggca gcgaacccgg
     2341 tgaacgagaa gtgattagcc gacacgttgg tccattggtt gagacaaggt aacccgggcg
     2401 aagaacaggc ggcagccaag gttgatatca atgggacgca gggccaattt ggcggcaacg
     2461 taaccccaag ggacgggacc cgcatgagca agttgctctc gtggcttgta tggatcgtcg
     2521 tcgcgctcac gggcgcaggc gcattcgcgt ggctcg
//
LOCUS       DATLBZ010000199        13822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5412841, whole genome shotgun sequence.
ACCESSION   DATLBZ010000199 DATLBZ010000000
VERSION     DATLBZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13822
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5412841"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..3892)
                     /locus_tag="VMZ53_10375"
     CDS             complement(200..3892)
                     /locus_tag="VMZ53_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28910.1"
                     /translation="MLALVFIAANVTELVVDVPAPRDTAALAAQQQGGTASYGVLRTK
                     QSGIPLWRNEGCINVIYNSTSVSIDEARALDAAFAAWSDAGLACGRIAVTSSRTGNPP
                     SSIDGRNTVHIRRDRWCKPNDSVGPEHCYPHEVSAITRLVFVDDPADPDDGKITEFDM
                     ELNAVDFVLLAPNATAPMTTKTVLDLQAVATHEAGHALGLAHDCGTGAEPWPMDHAGN
                     AVPACDAASPAVRAATMFYAVGANDLGPRTVEPAEVTGMCEVAKDLTCEEVVEGGCSA
                     GGSPGLLVLIVLAAAGGGMRSRRRPGVTGSRYHRAFLMDAPIPQRIQRFQIHALIGRG
                     GMGSVYRARDPQLERDVAIKVLSDSNRSMTQQLSADDTLDLREDGPATADELLREARM
                     MAQLSHPNVLPVYEVGLHDGSVFVVMEHIDGSDLADWLLKPRSTAEILTVFTQAAHGL
                     AAAHARGIVHRDFKPANVLVGADGRARVADFGLSRMNAPASAMVRVDDGRGTPRYMAP
                     ELWRGETASLKSDVYAFCAALDDALGGEQDERADARDKRWREKSLSPKLREVLAAGLS
                     ERPSARPELPQIVIALGGPAHRGRWLLLGGAGGAAAAAAIAFVVLAPARKSTPPPCEI
                     ASPAVFPVPPAFWTDFEQKRSALSSAITTTCKAFHDGELTAGQVEVRAHCYQRRDIEL
                     QTLAHLLIVDPSSVGDQGAIMQQFANPLQCEQIDETFIVDVTKLRALYTRYFHSFGLA
                     TPEKSKQHEELLIDLAQEAAATGERHLQGRVLSELGYELRYQDRLKEADAAWKEAGQI
                     GTLIHAPSLTVFSYVERSFTASLANDDKSATAYAEMAQGLAAKSGPLINANVLHAIGR
                     ARNKAADYKQGGELLREAFAAIQQVPPPGDPLLELNIRSALCEALEMSHQAEASVKLA
                     KETVEWAKKNFNERSDSYGGAVESLAAAQQAAGDREAALVTRLQALEIMKLTNPPTSA
                     RIALEHLTVSSDLMELGRFEEARREIQTAVDITEHNERARKELPWMLGSLASATWDAG
                     DHEGGLKIAQRALNETIVLRGGDSASTQDARLRVTDMLFELGRLDAAEKQLVMFEESL
                     RRSGKDLILARLQMAFRVELLNRSGKGKDAEKLARELQTTINELGAVEGDMYAFHAHI
                     ADALRVQKRYEEAHAEALQALEMAKKMKMRADYIALDEVRLARIEDKSGKHEQAVARA
                     KSVLELFKTYRGQPLAQREAQKILAQ"
     gene            complement(4038..5348)
                     /locus_tag="VMZ53_10380"
     CDS             complement(4038..5348)
                     /locus_tag="VMZ53_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS28911.1"
                     /translation="MDRATLAVRGQTILVRQFEVTVLDGTDRGARAVSSSDELSIGTA
                     EGNDLQLTDPAVSRHHCALRAEARGLSLTDLGSRNGTFVSDVETLSCFVKSGAQIRIG
                     KTTLGVRILDHDLEQPIASTNRLGPLVGASPAMRRLYPMLEQCGASTATVLVYGETGT
                     GKELIAEAIHGASERKKKPFVVIDCSALSHDLAESELFGHEKGAFTGAEYQRIGAFES
                     ANGGTIFLDEIGELPLELQPKLLRVLEARTIRRVGSNEQRPVDVRVVAASHRDLRALV
                     NDKRFRPDLFYRLNVVRIVVPALREREGDIELLARTFWQTFRPGGEPPQGLIPHLAAQ
                     SWPGNVRELRNAVERAALLGWRAEQAPASEADTLTYQQAKERAVWEWERGWVEKLIAA
                     HGGNLSRAARATKMGRSHLREIARRHGVVTRASATEIDPDTDED"
     gene            complement(5362..6504)
                     /locus_tag="VMZ53_10385"
     CDS             complement(5362..6504)
                     /locus_tag="VMZ53_10385"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HUS28912.1"
                     /translation="MKGKFRALVAHPQTEALGELAELLGTTGLAVDTARSTVEALNKL
                     RANQHHVIISFHVAGAFDGVAMLEASVLHAPEALRIALVSSPDEAVELDYRPAHGGTI
                     IRLIARPSERSRLVALVNEGVKLLSLVEQQRELVKRLSLDQGKLQRRELLLDTVVKER
                     TKELEESYEKLKAANRQALFGLAEAIEAKDPYTKGHCGRVAAYSLVLAREAGYPVDGL
                     ETLEFGAFLHDIGKIGIKDAVLLKPGPLDDAEWTHMREHPLKGFEIASKIEMLKPIMS
                     AVRNHHERWDGTGYPDKMVSNDIPLAARIVAIADAFDAMATDRPYKKALPLEECEAVL
                     RKTAGKMYDPDLIEVFVSRHLGELYREDYGDEHYDDGHFDAEAASK"
     gene            6558..8207
                     /gene="hflX"
                     /locus_tag="VMZ53_10390"
     CDS             6558..8207
                     /gene="hflX"
                     /locus_tag="VMZ53_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HUS28913.1"
                     /translation="MAEEVTGNTQGLKASEIKALERLYRRRVAPTDVVSPELATQLAT
                     LTAELHRQVGVLIDRRGAIEHVMVGDASKIFLPDFGRIRGGSGRFRGLRLVHTHLRGE
                     SLTRDDLTDLALLRLDAVAAITVDATGRALKLFLAHMQPDGPAAELPAESPHAPKTNF
                     LELITRVEQQFGRAHRVAATDRGKDRALLVHVDIGRSRDSDARVAELRELCRTAGVKV
                     LDVIVQRRPEADPKFLVGRGKLDEILLRAMQLGAECVIFDPDLTPGQARMISDVTEVK
                     VLDRTMLILDIFAQHATSRDGKLQVELAQLRYTLPRLIEKNTMMSRLTGGIGGRGPGE
                     TKLEINRRRARDRINLLEKRLEGLADDRRLRRRRRGEREVPIIAICGYTNAGKSTLLN
                     SLTEGDAIAADKLFATLDPISRRLRFPEEREVVITDTVGFIRDLPKELVAAFRATLEE
                     MEDADLLVHVVDASDADRDQHIKAVSEILNDLGLAEKPRIIVWNKADQLEEADAEALA
                     RGGGFVVSALDRATFGPLLLAIERALWNQGKDTHVARALHA"
     gene            8227..9210
                     /locus_tag="VMZ53_10395"
     CDS             8227..9210
                     /locus_tag="VMZ53_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28914.1"
                     /translation="MLRVLALCLGVAACGRIGFDPGSSDGNASDGARVDVITGPQVVA
                     SFTPRARNIAINATDVYWTNDDFTVMKCPLSGCGTTPTTLATNQFRADGIAINATGVY
                     WANYNDSVPSTVVGCSLSGCGAAPTTIASNQIGPTSIVADASNVYWSNFNDPSVQQCS
                     TTTCNDTPIPLAVGFLAPWSLTVDGATVYFLQNTGTGGVFTCPVSSCPAGPTRLTSSQ
                     INPGDLVLDATHIYWTEFGGNRVWRCDRAACMTTGTVLADSESNPSAIAVDNTTVYWT
                     DQLGRIRTCGLDGCAKNPTTLISGQTGIGGIKVDANAIYWARDGAIMKLAK"
     gene            complement(9211..10101)
                     /locus_tag="VMZ53_10400"
     CDS             complement(9211..10101)
                     /locus_tag="VMZ53_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUS28915.1"
                     /translation="MEWLNFHHLRYFWVVAREGSVSRAAKRLRVTQPTISGQLRELQD
                     SLGEPLFRRAGRQLALTDVGKSVLEIADEIFALGDQVLDTIKGKATGRPQRLAVGISD
                     AVPKRVAYRILEPAIAARVQIACHEGPPARLVAELAAQSLDVVISDAPSHEARAHDHL
                     LGESRISVWGVRRLAAEMRVGFPRSLDGAPFLLPSANTGFRRELDAWFVEHGIRPRVV
                     GEFEDAALLAEFAEAGAGLYATPDVVVEASKPRGLQRAGGIRPLRARYYAITVERRLA
                     HPAVALIAGAEKLFGRRAAR"
     gene            10187..11059
                     /locus_tag="VMZ53_10405"
     CDS             10187..11059
                     /locus_tag="VMZ53_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HUS28916.1"
                     /translation="MAEPTLKSALARTGFWIALAVLFGLGVTAHLGTQRGMEFFTGYL
                     LEQALSVDNLFVMMLVFARFQVPKAAQRRVLTWGIAGVVLLRGVMIFGGTALVGTFHA
                     LTYVLGAVLLYSAYKLARDLGDDDHGAGDDDSTASIGMRVRRVVGRLIPISDSFAGDR
                     FTVVLRGIRHATPLLLALVTIEIADAIFALDSIPAVFGVTTDRFIVFSSNLFAVLGLR
                     SMYFALSGLFERLRFLQHGLVGVLGFIGLKMLVAAVWTVPAWFALAAVALILGGAIAA
                     SLLIPQKELNAEQS"
     gene            11043..11642
                     /locus_tag="VMZ53_10410"
     CDS             11043..11642
                     /locus_tag="VMZ53_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS28917.1"
                     /translation="MLNNRDYTIIIDKSGSMATKDQSGQRSRWAAARESTEAFARRCE
                     ELDPDGLTLYVFASKFRRYERVTADRVSAVFVENEPCGGTDMAAVLDHSFDAYFQRKG
                     SGDARPETVLVVTDGAPDDEKAVMKTVIRAAKRVQRADELVVSFIQIGTDGGARRFLK
                     ILDDEMVRAGAPFDIVTTVTLDEAEGIGLTEVLMTALRQ"
     gene            complement(11644..13407)
                     /locus_tag="VMZ53_10415"
     CDS             complement(11644..13407)
                     /locus_tag="VMZ53_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HUS28918.1"
                     /translation="MKRLAYVMLSACSVHAGTGDDDTPPLECEPTTVVAPGGDAFVDI
                     SAQSGIQANNFTPSPTTTIPINDHSRLAFIDLDGDRCDDIVAHDLFPNPQAGVPYTHL
                     VFHSNCDGTFSDASASSGLRDIQAGFFAFGDLDNDGDQDCFAGLDIPLAGKANQILLN
                     DGQGHFTPKASSGVEGPIGNTSAANAVVADFNGDAKLDLFIGNGQTSYLALDGLFFGN
                     GDGTFTNATTKLTGNAASPSNGSTACDYDNDGDLDIFVSVYGVSNGGAQNFLYQNDGH
                     GSFKNVAVEAGFASLPTGNYWINATGMGTLREPNASPGHFIGSNGFGLACEDADRDGD
                     LDVTVSAISHPGGDYSRTWSDPTVLLVNTGAAGGFTFENAWLTKRLPYNEGDVDAAMV
                     DFDNDGRMDISLSRTDKYESGYQTENQKGWFGLMHQTPDGMFEVLDRSAGLTDEDHGP
                     GMKGAQNHAWSDIDRDGDLDLLIGARDQGGGRANLLFRNDVGHKNQWLSLAIVGDGSH
                     INRDAIGTRVTLTFADGTQLVREVKASRGTYNSMDTRQLYFGLGDLGCGYTVEVRFPD
                     GTTRSLPASDVPPNHAATITY"
     gene            complement(13484..>13822)
                     /locus_tag="VMZ53_10420"
     CDS             complement(13484..>13822)
                     /locus_tag="VMZ53_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28919.1"
                     /translation="GAGLVVQLAADGKVTWARRFPSQPYLANVNGRWIAAYTTTNDVA
                     VVEIDLKGGADVALGVIEGAGVQDAMDIAAVGPDAVAVTVNTRGVLTFGATSFDSGAI
                     TLRAVAVLGI"
BASE COUNT         2248 a         4553 c         4531 g         2490 t
ORIGIN      
        1 gcgccggtgc attgaatgcc agcgcacgca gcgagcacgg cgtccccact ggcatcgccc
       61 tgcacggcgt cgcttgctac gccatcggtt gcgtcgcgtg tgcccgaccg ttcatcgaat
      121 gcgagccggc cgcatgccgt cgatacgagc gccgcacaca cagcccatcg cacacctcaa
      181 cggtaccagc gccaacgagt tattgcgcga ggatcttctg ggcttcacgc tgcgcaagtg
      241 gctggccgcg gtacgtcttg aacagctcca gcacgctctt cgcgcgcgcg acggcctgct
      301 cgtgcttacc gctcttgtcc tcgatgcgcg cgagccggac ttcatcgagt gcgatgtaat
      361 cggcgcgcat cttcatcttc ttggccatct cgagcgcttg gagcgcttcg gcgtgcgcct
      421 cctcgtagcg cttctgcact cgcagcgcgt cggcgatgtg ggcatggaac gcgtacatat
      481 cgccttcaac cgcgccgagc tcgttgatcg tcgtctgcag ctcgcgcgcg agcttctcgg
      541 catccttgcc tttgccgctg cgattgagca gctccacgcg aaaggccatc tgcagccgcg
      601 cgaggatgag atccttgccg gatcgtcgca gcgactcttc gaacatcacg agttgcttct
      661 cggctgcatc gagtcgtccg agctcgaaca acatgtccgt caccctgagg cgcgcgtcct
      721 gcgttgacgc cgagtcgcca cctcgtaaga caatcgtctc gttgagtgca cgctgggcga
      781 tcttgagtcc gccttcgtga tcaccggcat cccacgtcgc cgacgcgagc gaccccagca
      841 tccatggcag ctccttgcga gcgcgttcgt tgtgctccgt gatgtcgacc gccgtctgga
      901 tttcgcgccg cgcttcctcg aatcgaccga gctccatcag gtcactcgac acggtgaggt
      961 gctcgagagc gatacgcgcg ctcgtcggcg gatttgtgag cttcatgatc tcgagcgcct
     1021 gcaagcgcgt gacgagcgct gcttcacggt cccccgccgc ctgctgagcg gccgccagcg
     1081 attcgacggc tccgccgtag ctgtccgagc gttcgttgaa attcttcttc gcccactcga
     1141 cggtttcctt cgcaagcttc accgacgcct cggcttgatg tgacatctcg agcgcctcac
     1201 atagggcgct gcggatgttg agctcgagaa gcgggtcacc ggggggcggc acctgctgga
     1261 tggccgcaaa cgcctcccgc agcagctcgc ctccctgttt gtagtcggct gctttgttcc
     1321 gcgcgcgacc gatcgcatgc agcacgttcg cgttgatgag cggccccgat ttggcggcga
     1381 ggccctgtgc catctcggca taggcggtcg cgctcttgtc atcattggcg aggctcgccg
     1441 tgaagcttcg ctcgacgtac gagaacacgg tgagcgacgg cgcatggatc agcgtgccga
     1501 tttgaccggc ctccttccac gcggcgtcag cctctttcaa acggtcttgg tagcgcagct
     1561 cgtatccgag ctcggacagt acgcggcctt gcagatggcg ttcaccggtc gccgcggcct
     1621 cttgcgccag gtcgatgagt agctcttcgt gttgcttcga tttctccggc gtcgcgagac
     1681 cgaatgaatg gaagtagcgc gtgtagagcg cgcggagctt cgtgacgtcg acgatgaacg
     1741 tctcgtcgat ttgctcgcac tgtagagggt tcgcgaactg ctgcatgatc gcgccctgat
     1801 cgccgacgga gctgggatcg acgatcagca ggtgtgcaag cgtctgcaac tcgatgtcgc
     1861 ggcgctgata gcagtgggcg cgcacctcga cctgaccagc cgttagctct ccgtcgtgaa
     1921 acgccttgca cgtcgtcgtg atcgcactgc tcagcgcgga gcgcttctgc tcgaaatccg
     1981 tccaaaacgc cggcggcacg ggaaatacgg ctggactggc gatttcacat ggcggcggtg
     2041 tcgatttgcg cgccggcgcg agcacgacga acgcgatcgc cgcagcggct gctgcaccac
     2101 ctgcgccgcc gaggagcagc cagcgcccgc gatgagcggg accaccgagc gcaatgacga
     2161 tctgcggcag ctcgggccgt gcgctcggtc gctccgagag gccggcggcg agtacctcgc
     2221 gcaacttcgg cgacagactt ttctcgcgcc atcgcttgtc gcgcgcgtcc gctcgttcgt
     2281 cctgctcgcc gccgagcgcg tcatcgagcg ccgcgcagaa cgcgtagacg tccgacttta
     2341 gtgatgccgt ctcgccgcgc catagctcgg gtgccatgta gcgcggcgtg ccgcggccat
     2401 cgtcgacgcg aaccatcgca cttgccggcg cgttcatgcg cgataggccg aagtccgcaa
     2461 cgcgtgcgcg accgtcggcg ccgacgagca cgttggccgg cttgaagtcg cgatgcacga
     2521 tgcctcgcgc gtgcgcagcc gcgagaccat gcgccgcttg cgtgaacacc gtcaggattt
     2581 ctgcggtcga gcgcggcttc aggagccagt ccgcgagatc ggagccgtcg atgtgctcca
     2641 tcacgacgaa cacagagccg tcgtgcagcc cgacttcgta gaccgggagc acattgggat
     2701 gcgagagctg cgccatcatg cgtgcttcgc ggaggagctc gtcggcggtt gccgggccgt
     2761 cttcgcgcag gtcgagcgtg tcgtcggcgg acagctgctg cgtcatcgag cgattgctgt
     2821 cgctgagcac cttgatcgca acgtcgcgct cgagctgcgg atcgcgcgcg cgatacaccg
     2881 agcccatccc gccgcgcccg atgagcgcgt ggatctggaa ccgctggatg cgctgtggga
     2941 tcggtgcgtc catcaggaac gctcgatggt atcgcgagcc ggtcacgcct ggccgacggc
     3001 gcgaacgcat cccgccaccg gcggcggcta gaacgatcag gacaagcagc ccgggcgatc
     3061 cacccgcact gcagccacct tcaacgactt cttcgcacgt gaggtccttc gcgacctcgc
     3121 acatgcccgt tacttcggcc ggctcgacgg tgcgtgggcc aaggtcattg gcgccgacgg
     3181 cgtagaacat cgtcgctgcg cgcacggcgg ggcttgccgc gtcgcacgcc gggacggcat
     3241 tgcctgcgtg atccatcggc cacggttcgg cgccggtgcc acagtcgtgc gcgaggccga
     3301 gagcgtgacc ggcttcgtgc gtcgcgacag cctgtagatc gaggaccgtc ttggtcgtca
     3361 tcggagccgt cgcattcgga gcgagcagca cgaagtcgac cgcgttcagc tccatgtcga
     3421 actcggtaat cttgccgtcg tcgggatcgg ccggatcgtc gacgaacacg agccgcgtga
     3481 tcgcggagac ctcgtgcgga tagcagtgct cgggcccgac gctgtcgttc ggcttgcacc
     3541 agcgatcgcg gcgaatgtgg acggtgttgc gaccgtcgat ggaggagggc gggttgccag
     3601 ttcgcgagct cgtcaccgcg atgcgcccgc atgcgagtcc ggcgtcactc catgccgcga
     3661 acgccgcgtc gagtgcacgc gcttcgtcga tcgagaccga cgtcgagttg tagatgacgt
     3721 tgatgcagcc ttcgttccgc cacagcggga tgccgctctg cttcgtgcgg agcacgccgt
     3781 agctggcggt gccgccttgc tgctgtgcag cgagcgccgc ggtgtctcgt ggcgcgggga
     3841 cgtcgaccac aagctcggtg acgttcgccg cgatgaagac gagagcaagc ataggcgtcg
     3901 tccgttagag caacggtggg gccacgccgg ggtggtctgg cgccggacca ccgaaatcca
     3961 atggaatcgc gcgctcctgc aggcgtggcg accgcgcccc cgcgggggag tcgcagcccc
     4021 accggtctag cgaaggctca gtcctcgtcg gtatctggat cgatctcggt ggcgctggcg
     4081 cgagtgacga cgccatgtcg ccgcgcgatc tcgcgcaagt ggctgcggcc catcttcgtg
     4141 gcgcgggcag cgcgcgagag gttgccgcca tgcgcggcga tcagcttctc gacccagcca
     4201 cgttcccact cccacaccgc gcgctctttc gcttgctgat acgtcagtgt gtcagcctcc
     4261 gacgccggcg cttgctccgc gcgccacccg agcagcgctg cacgctcgac ggcgttgcgc
     4321 agctcgcgaa cattgcccgg ccagctctgc gccgcgagat gcgggatgag gccctgcgga
     4381 ggctcaccgc cggggcgaaa cgtctgccag aacgtgcgcg cgagaagctc gatgtcacct
     4441 tcgcgctcgc gcagcgcggg cacgacgatg cgaacgacgt tgagtcgata gaacagatcg
     4501 ggacgaaacc gcttgtcgtt gacgagcgcg cggagatcac gatggctcgc cgcgacgacg
     4561 cgaacatcga cgggccgctg ctcgttgctg ccgacgcgcc gaatcgtgcg ggcctcgagc
     4621 acgcgcaaca gctttggctg cagctcgagc ggcagctcgc cgatctcgtc gaggaaaatc
     4681 gtgccgccgt tcgcgctctc gaacgcgcca atgcgttggt actcggcgcc cgtgaacgcg
     4741 cccttctcgt gaccgaacag ctcgctctcc gcgaggtcat gcgagagcgc gctgcaatcg
     4801 atgacgacga acggcttctt cttgcgctcg ctcgcgccgt ggatcgcctc ggcgatgagc
     4861 tccttgcccg tgccggtctc gccatagacg aggacggtcg cggtcgacgc gccgcactgc
     4921 tcgagcatcg gatacaggcg acgcatggcc gggcttgctc caacgagcgg gccgagccga
     4981 ttcgtcgacg cgatcggttg ctcgagatcg tggtcgagga tgcgtacgcc gagcgtcgtc
     5041 ttgccgatgc ggatttgcgc gccggatttc acgaagcacg acagcgtctc gacgtcactg
     5101 acgaacgtac cgttgcgact tccgaggtcg gtcagcgaga gcccacgcgc ttcggcgcgg
     5161 agtgcacagt gatggcgcga cacggcgggg tccgtgagct ggaggtcgtt gccctcggcc
     5221 gtgccgatcg acagctcgtc cgagctcgat actgcgcgcg cgccgcgatc cgtgccgtcg
     5281 agaacggtga cctcgaattg gcggacgaga atcgtctgac cgcgtacggc gagcgtcgct
     5341 cgatccatcg ctaggacggt atcacttcga tgcggcttcg gcgtcgaagt ggccgtcgtc
     5401 gtaatgttcg tcgccgtagt cctcgcggta gagctcgccg aggtggcgcg agacgaagac
     5461 ctcgatcagg tcagggtcgt acatcttgcc ggccgtcttg cgcagcaccg cttcacactc
     5521 ttcgagcggc agtgccttct tgtaggggcg atcggtcgcc atcgcgtcaa atgcgtcggc
     5581 gatcgcgacg atgcgcgcgg cgagcgggat gtcgtttgac accatcttgt cggggtaccc
     5641 ggtgccgtcc cagcgctcgt ggtgattgcg caccgcgctc atgatcggct tgagcatctc
     5701 gatcttcgac gcgatctcga aacccttgag cgggtgctcg cgcatgtgcg tccactcggc
     5761 gtcgtcgagc gggccgggct tcagcagcac ggcgtctttg ataccgatct tgccgatgtc
     5821 gtgcaggaac gcgccgaact cgagcgtctc gagaccatcg acgggatatc cggcttccct
     5881 cgcgaggacg agcgagtacg cggcgacgcg cccgcagtga cccttcgtgt acgggtcctt
     5941 cgcttcgatc gcctcggcga ggccgaacag cgcctggcgg ttagcagcct tgagcttttc
     6001 gtagctctcc tcgagctctt tcgtgcgctc tttgacgacg gtgtcgagga gcagctcgcg
     6061 gcgctgcagc tttccttgat cgagtgacag acgcttgacg agctcgcgct gctgctcgac
     6121 gagcgagagc agcttcacgc cttcattgac gagggcgacg agccggctcc gttcgctcgg
     6181 acgcgcgatg aggcggatga tcgtgcctcc atgcgcgggg cgatagtcga gctcgaccgc
     6241 ttcgtccggt gatgacacga gcgcgatgcg cagcgcctcg ggggcgtgca gcacgctggc
     6301 ttcgagcatg gcgacgccgt cgaaggcccc cgcgacgtga aacgagatga tgacgtgatg
     6361 ctggttggca cggagcttgt tgagggcctc gaccgtcgag cgtgcggtgt cgaccgccag
     6421 cccggtggtg cccagcaatt ccgcgagctc accgagggct tccgtctgcg ggtgagcgac
     6481 cagcgcacgg aatttcccct tcacgatgct gactatatcc ctggcccttc gcgctgcaac
     6541 gggtagattc cacgcgcgtg gcggaagaag taacgggcaa tacgcaaggt ttgaaggcaa
     6601 gcgagatcaa ggcgctcgag cgcctttatc ggcgccgtgt tgcgccaaca gatgtcgttt
     6661 cgcccgagct cgcaacacag ctcgcgacgc tcactgcgga gctacatcgc caagtcggcg
     6721 tcctgatcga ccggcgcggt gcgatcgagc acgtgatggt cggcgatgca tcgaagatct
     6781 ttctgccgga cttcggtcgt atccgcggag gcagtggacg ctttcgtggc ttgcgtctgg
     6841 tgcacacgca ccttcgcggc gagtcgctaa ctcgtgacga tctcacggac ctcgcgctac
     6901 tgcgtctcga cgccgtcgct gcgatcaccg tcgacgcaac gggccgtgcg ctcaagctct
     6961 tcctcgcgca catgcagccc gatggcccag ctgcggagct gccggccgaa tcgccgcacg
     7021 cgccgaagac aaacttcctc gagctcatca cgcgagtcga gcagcaattt ggtcgcgcgc
     7081 atcgcgtcgc agctaccgat cgcggcaaag accgcgcgct cctcgtccac gtcgatatcg
     7141 gccgctctcg cgatagcgat gcgcgcgtcg ccgagctgcg tgagctgtgc cgaacggcgg
     7201 gcgtcaaagt cctcgacgtg atcgtccagc gccgccccga ggcagatccc aagttcctcg
     7261 tcggtcgcgg caagctcgac gagatcctgc tgcgcgcgat gcagctcggc gccgagtgcg
     7321 tgatcttcga tccggatctc acgccgggcc aagcccgcat gatcagcgac gtgacggagg
     7381 tgaaagtcct cgatcgcacg atgctgatcc tcgacatctt cgcgcagcac gcgacgtcgc
     7441 gcgacggcaa gctgcaagtc gagctcgcac agctccgcta caccctcccg cgcctgatcg
     7501 aaaagaacac gatgatgtct cgcctgacag gcggcatcgg cggccgtggt cccggcgaaa
     7561 cgaagctcga gatcaaccgc cgccgcgctc gcgatcgcat caacctcctc gagaaacgcc
     7621 tcgaaggtct cgccgacgat cgccggctcc gccgccgccg ccgcggtgag cgcgaggtcc
     7681 cgatcatcgc gatctgcggc tacacgaacg ccggcaagtc gacgctactc aactcgctca
     7741 ccgagggcga cgcgatcgca gccgacaagc tgttcgcaac gctcgacccg atctcgcggc
     7801 gcctccgctt cccggaagag cgcgaggtcg tcatcaccga cacggtcggc ttcatccgcg
     7861 atctcccgaa ggagctcgtc gcagcgttcc gagccacgct cgaagagatg gaagacgccg
     7921 acctgctcgt gcacgtcgtc gatgcgtccg atgcagaccg cgaccaacac atcaaggcgg
     7981 tctccgagat cctgaacgac ctcggcctcg ctgagaagcc gcgcatcatc gtgtggaaca
     8041 aggccgacca gctcgaagag gccgatgccg aagcgctcgc tcgtggtggc ggcttcgtgg
     8101 tctcggcgct cgaccgcgcg acgttcggcc cgctgctgct cgcgatcgaa cgcgcgctct
     8161 ggaatcaggg caaagacacg cacgtcgcgc gcgctctcca cgcctgagcg cggttatgct
     8221 gaacgcgtgt tgcgggtgct ggctctttgc ctcggcgtag ctgcgtgcgg ccgcatcggc
     8281 ttcgacccgg gcagctccga cggcaacgca agcgacggcg ctcgcgtcga cgtcatcacc
     8341 ggcccacaag tcgtcgcgtc atttacgccg cgcgcgagga acatcgcgat caacgcgacc
     8401 gatgtctact ggacgaacga cgacttcacc gtgatgaagt gcccgctctc cggctgcggc
     8461 acgacaccga cgaccctcgc taccaatcaa ttccgcgccg atggaatcgc gatcaacgcc
     8521 acgggcgtct actgggccaa ctacaacgac agcgttccga gcactgtggt gggctgcagc
     8581 ctctcgggct gtggcgccgc gccgacgacg atcgcctcca atcagattgg acccacgtcg
     8641 atcgtcgccg atgcgagcaa cgtgtactgg tcgaacttta acgatccttc cgtgcagcag
     8701 tgctccacca cgacctgcaa cgacacaccg atcccgctcg cagtcggctt cttggccccg
     8761 tggtcgctca ccgtcgatgg cgcgaccgtc tactttctcc agaacaccgg tacgggtggc
     8821 gtcttcacct gcccggtaag cagctgcccg gcggggccaa cgcgcctcac cagcagccag
     8881 atcaatccag gcgacctcgt gctcgatgcg acgcacatct actggaccga gttcggcggc
     8941 aaccgcgtct ggcgctgcga ccgcgcggcg tgcatgacca ccgggaccgt cctcgcagac
     9001 agcgagagca acccgagcgc gatcgcggtc gacaacacga cggtctactg gacagaccaa
     9061 ctggggcgta ttcgcacgtg cgggctcgat ggctgcgcga aaaatccgac gacgctgatc
     9121 tccgggcaga ccggcatcgg cggcatcaag gtcgacgcaa atgcgatcta ctgggcgcgc
     9181 gatggcgcga tcatgaagct cgcgaagtaa ctagcgcgcg gcgcgacgtc cgaacaattt
     9241 ctcggcgccc gcgatgaggg cgacagcggg atgggcgagg cggcgctcga ccgtgatcgc
     9301 gtagtaacgg gcgcggagcg ggcggatgcc gccggcgcgc tggaggccgc gaggcttgct
     9361 cgcctcgacg acgacatcgg gtgtggcgta gagaccagcg cccgcttcgg caaactccgc
     9421 gagcaatgcc gcatcttcga attcgccgac gacacgcggc ctgatgccgt gctcgacaaa
     9481 ccacgcatcg agctcgcggc gaaaaccggt attcgcgctc ggcagaagga atggcgctcc
     9541 gtcgagtgaa cgcgggaagc cgacgcgcat ctcggcggcg agacggcgaa caccccacac
     9601 gctaatgcgc gactcaccga gcaagtggtc gtgcgcgcgc gcttcgtgcg agggcgcgtc
     9661 gctgatcacg acgtcgagcg actgcgctgc tagctcggcg acgaggcgtg cgggcggtcc
     9721 ttcatggcac gcgatctgga cgcgcgcggc gatcgcaggc tcgaggatgc gatacgcgac
     9781 tcgcttgggg acggcgtcgc tgatgccgac ggcgaggcgc tgcggccggc ccgtcgcctt
     9841 gcctttgatg gtgtcgagga cctggtcgcc gagcgcaaag atctcgtcgg cgatctcgag
     9901 gactgacttg ccgacatccg tgagagcgag ttggcgaccg gcgcggcgga acagcggctc
     9961 accgagcgag tcttggagct cgcggagttg gccggagatg gtcggctgcg tcacgcgcaa
    10021 tcgtttcgcg gctctcgaca cgctgccttc gcgagcgacc acccagaagt agcgaaggtg
    10081 gtgaaagttc agccattcca tcccgatcaa tatatcgaga aaaccgatgt gacggcgcca
    10141 tttatcgatt tgtcaggcgt gcgaagccga cccaaatcta gtcttcatgg cagaacccac
    10201 gctgaagtcg gcgctcgcgc gcaccggctt ctggatcgcg ctcgctgtct tgttcggtct
    10261 cggcgttacc gcccacctcg gcacccagcg aggcatggaa ttcttcacgg gctatctgct
    10321 cgagcaggcg ctgtccgtcg acaacctgtt cgtcatgatg ctcgtcttcg cgcgcttcca
    10381 agtgccgaag gcggcgcagc gtcgcgtgct cacgtggggc attgctggcg tcgtgctgtt
    10441 gcgcggcgtg atgatcttcg gtggcaccgc gctcgtcggg acatttcacg cgctcaccta
    10501 tgtgctcggc gcggtgctgc tgtactcggc gtacaagctc gcacgcgacc tcggcgacga
    10561 cgaccacggg gcgggcgacg atgactcgac agcgagtatc gggatgcgcg tacgccgcgt
    10621 cgttgggcgc ctgatcccga tcagcgactc gtttgccggc gatcgcttca cggtcgtgct
    10681 gcgcgggatt cgccacgcga cgccgctttt gctcgcgctc gtcacgatcg agatcgcgga
    10741 cgccatcttc gcgctcgatt cgattcccgc cgtcttcggc gtcacgaccg atcgcttcat
    10801 cgtgttctcg tcgaacctgt tcgcggtcct cggcctgcgc tcgatgtact tcgcgctctc
    10861 cggcctgttc gaacgcctcc gcttcctcca gcacggcctc gtcggcgtcc tcggcttcat
    10921 cggcttgaag atgcttgtcg cggccgtgtg gacggtgccg gcctggttcg cactggcggc
    10981 cgttgctctc atcctcggcg gcgccattgc ggcgtcgctc ctcattccgc agaaggagct
    11041 caatgctgaa caatcgtgac tacaccatca tcatcgacaa gagcggcagt atggcgacga
    11101 aggaccagtc ggggcagcgc agccgctggg ccgcagcacg cgagagcacc gaggcgttcg
    11161 cgcggcgctg cgaggagctc gaccccgacg ggctgacgct ctacgtcttc gcgagcaagt
    11221 tccgccgcta cgagcgcgtg accgcggacc gcgtgtcggc tgtattcgtc gagaacgagc
    11281 catgcggcgg aacagacatg gccgccgtgc tcgatcattc gttcgacgcc tacttccagc
    11341 gcaagggtag cggcgatgcg cgacccgaaa ccgtgctggt cgtgactgat ggagcacccg
    11401 atgacgagaa ggccgtgatg aagacggtga ttcgagcggc gaagcgcgtg caacgcgccg
    11461 acgagctcgt cgtgtcgttt atccagatcg gaacggacgg cggagcgcgc cgatttctca
    11521 agatcctcga cgacgagatg gttcgggccg gtgcgccgtt cgacatcgtc acgacggtga
    11581 cgctcgacga ggccgagggt attggcctca ccgaagtgct catgaccgca cttcgccagt
    11641 gagctaatac gtgatcgtcg ctgcgtggtt cggcgggacg tcggaagcgg gcagggagcg
    11701 cgtcgtgccg tccggaaacc ggacctcgac ggtgtaaccg cagcccaggt cgccgaggcc
    11761 gaagtagagc tggcgggtgt ccatcgagtt gtacgtgcca cggctcgctt tgacttcgcg
    11821 gacgagctgc gtgccatcgg cgaacgtcag cgtgacgcgc gtgccgatcg cgtcgcggtt
    11881 gatgtggctt ccgtcgccca cgatcgcgag cgagagccat tggttcttgt ggccgacgtc
    11941 gttgcggaac aggaggtttg cccggccgcc gccttggtca cgcgcgccga tgaggaggtc
    12001 gaggtcgccg tcgcgatcga tgtcggacca cgcgtggttt tgcgccccct tcatgccggg
    12061 accgtgatct tcgtcggtga gaccggcgga gcggtcgagc acctcgaaca tgccgtcagg
    12121 cgtctggtgc atgaggccga accagccctt ctggttttcc gtctgataac cggactcgta
    12181 cttgtccgtg cgcgacagcg agatgtccat gcgaccgtcg ttgtcgaagt cgaccatggc
    12241 cgcgtcgacg tcgccctcgt tgtacggcag gcgcttcgtg agccacgcat tctcgaacgt
    12301 gaagccgccg gccgcgcccg tgttgacgag gagcaccgtc ggatccgacc acgtccggct
    12361 gtaatcgcca cccggatgcg agatcgcgga cacggtcacg tcgagatcgc catcgcgatc
    12421 ggcgtcttcg cacgcgagcc cgaatccgtt gctgccgatg aagtggcccg gcgaagcatt
    12481 cggctcccgc agcgtgccca taccggtcgc gttgatccag tagttgccgg tcggcaggct
    12541 cgcgaagccg gcctcgacgg cgacgttctt gaacgagccg tggccgtcgt tctggtagag
    12601 gaagttctgc gcaccgccgt tggacacgcc gtagacggag acgaagatgt cgaggtcacc
    12661 gtcgttgtcg tagtcgcacg cggtgctgcc gttcgatggg ctcgctgcgt tgccggtgag
    12721 cttcgtcgtc gcgttcgtga aggtgccgtc tccgttgccg aagaacagcc cgtcgagcgc
    12781 gaggtagctc gtctgaccgt tgccgatgaa gaggtcgagc ttggcgtcgc cattgaagtc
    12841 agcgaccacc gcatttgccg cgctcgtgtt gccgatcggt ccctcgacgc cgctgctcgc
    12901 cttcggcgtg aagtggcctt ggccgtcatt gagcaagatc tgattcgctt tcccggcgag
    12961 cggaatgtcg aggccggcaa agcagtcttg gtcgccgtcg ttgtcgaggt cgccgaaagc
    13021 gaagaagccg gcctggatgt ctcgcagccc ggagctcgcg ctcgcatcgc tgaacgtacc
    13081 gtcgcagttc gagtgaaaga cgaggtgcgt atacggcacg cccgcctgcg ggttgggaaa
    13141 caggtcgtgc gcgacaatgt catcgcagcg gtcgccatcg agatcgatga atgcgagccg
    13201 gctgtggtcg ttgatcggga tcgttgtcgt ggggctcggc gtgaagttgt tcgcttggat
    13261 gccgctctgc gccgagatgt cgacgaacgc gtcgccgccc ggcgccacga ccgtcgttgg
    13321 ctcgcactcg agcggcggcg tgtcgtcgtc gccggtccct gcatggacag agcaggcact
    13381 gagcatgacg taggcgaggc gcttcatgcc gcggaccgta aacagcggca gtcgcggtca
    13441 cccacaaata caatcggtca tcgggcggct tgtcgagtag ccgctagatg ccgagcacgg
    13501 cgacggcacg cagcgtaatc gcgcccgaat cgaagctcgt cgccccgaat gtcagcactc
    13561 cccgcgtgtt caccgtgact gcaaccgcgt cggggccgac cgcagcgatg tccatggcgt
    13621 cttgcacgcc cgcgccttcg atgacgccga gcgcgacgtc ggcaccgccc ttgaggtcga
    13681 tctcgacgac cgcgacgtca ttcgtcgtcg tgtacgccgc gatccagcga ccgttgacgt
    13741 tcgcgagata cggctggctc gggaaccggc gtgcccacgt gaccttgccg tcggcagcca
    13801 gctgcacgac gagcccagcg cc
//
LOCUS       DATLBZ010000200        10878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_941276, whole genome shotgun sequence.
ACCESSION   DATLBZ010000200 DATLBZ010000000
VERSION     DATLBZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941276"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VMZ53_10425"
     CDS             complement(<1..600)
                     /locus_tag="VMZ53_10425"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28920.1"
                     /translation="MSESWDETTSIGTMRSEDLAKGGLVFRYNRFVYRTLGTHLANGG
                     MGTVYTLERRDDESGVEEAVVGKVFHSNYLYQLRTDEVTRRDHHNNLAAMARIAAIEH
                     PNILPTYISAPIADNYLFVTPRMGMTLLEAIGKHNLTPRARTKLLVQALEGLATLHAA
                     RLIHRDFTLRNILVDEGANVACLFDFDLAMSLDDVGNQTF"
     gene            722..1666
                     /locus_tag="VMZ53_10430"
     CDS             722..1666
                     /locus_tag="VMZ53_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HUS28921.1"
                     /translation="MLEHRFAKLAAGATFILLVIGGTVNPTGSSLACPEPTLVCHGQL
                     FPPMVGGVLYEHGHRLAAMTVGLLQIALTVALFQSRRRRLAVGLLCMILAQGALGAIT
                     VKYKLPWFISTFHLLLGMSYFATLIYTAFATRPAPSAMELERHEKLRAELGTARRWIA
                     IACGFVFVQLLLGALVRHFGAAMVCLGVPSCTRAGDWWTGLAPVQHVHMIHRGFGVIV
                     ALVTTVAAIAVYRRTKAWPQLRLLAMIAPVLVIAQVALGIYTVLTMRSVPVAVGHFAG
                     AASLWALWMSAWLMTARRQKAVAIAPERASATTEAVPA"
     gene            1663..2523
                     /gene="cyoE"
                     /locus_tag="VMZ53_10435"
     CDS             1663..2523
                     /gene="cyoE"
                     /locus_tag="VMZ53_10435"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HUS28922.1"
                     /translation="MTAKALTAKTVDLVALVKPNIMIMALLTAAGAMSLAPGTLPLGN
                     ALWLLAGTALIVGSANTLNMWLERDIDCLMSRTKNRPLPQGRLHATTALVFGAVQGAV
                     SIPLLAIVGIIPAALGLVALLLYVGAYTPMKQVSHRSVWVGGVPGAMPALMGWTAATG
                     HIDLAGLAVFGVLYIWQIPHTHAISIYREREYHAAGLKTLSGTHGATVARRYILAFLV
                     VQVAASLAPALLGVAGLPYLVTAIALGVMVLVQGFAGNGSAKWARNVFLTSIVYLPIL
                     FAVMVASGNA"
     gene            2530..2964
                     /locus_tag="VMZ53_10440"
     CDS             2530..2964
                     /locus_tag="VMZ53_10440"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HUS28923.1"
                     /translation="MSGHEIHPAINAALNLTSFVFLLLGRWEIARGNEAVHRRRMLTA
                     FTASCVFLVSYVIRFATTGTHKYPGTGWDKYLYLFILFSHMVLAIVLAPLLLKALRRA
                     LAADFVAHKRIVKYTFPIWVYVSATGVIVYLMLYHLAPALHA"
     gene            2966..3709
                     /locus_tag="VMZ53_10445"
     CDS             2966..3709
                     /locus_tag="VMZ53_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28924.1"
                     /translation="MPRWVDAGFWGLVSGSALVLGASVGYFVRVPARVIAAIMAFGAG
                     VLMSALSFDLLDAAYLRGGFDATAIGFLAGAAIYTAANYALARRGAKHRKRSGDQQPS
                     DRGSGAAIAVGALLDGIPESIVIGVSMIGGGAVSTAAVAAVFLSNVPEGMSSAAGMKR
                     AGRAPGYVFGVWGAIAAVSGVSALLGYSVFAGLSPNIIAATTAIAAGAILAMLSDTMM
                     PEAFEAAHDLAGLITVAGFLCAFVLTKLG"
     gene            complement(3711..4601)
                     /locus_tag="VMZ53_10450"
     CDS             complement(3711..4601)
                     /locus_tag="VMZ53_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28925.1"
                     /translation="MNSAVIDIGTNTLLLLIVNEQKQRVVDLCRFGRLGKGLDGSGNL
                     AAESIAKSLDICREYRTVMDDHGVETPRVIGTQALREAGNAAQFVGPAEQILRAKLEV
                     IGGAREAELAFLSVAKTMPELEGQAFIVVDVGGGSTEFIVSDGARVVSAVSIPIGAVR
                     LTERHLKTDPPSAAELAALASDIDDRLAALGLPTGLPIIGTAGTATTLAAVALKLVPY
                     DPDQVTGLRLTPEDVDGLYQRLARATVVERKAMPGMEPQRADVIAGGAAIYACIMKST
                     SAPVFVACDRGIRWGLVYET"
     gene            4693..4944
                     /locus_tag="VMZ53_10455"
     CDS             4693..4944
                     /locus_tag="VMZ53_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="HUS28926.1"
                     /translation="MRFDGEGTTAEHIVMLRWRQAIGDTNITDMISWIESGGIAKVQR
                     PGEDRIEDVKVVRESGRKPYLVTIADGRPTQSIIELPRF"
     gene            complement(4941..5396)
                     /locus_tag="VMZ53_10460"
     CDS             complement(4941..5396)
                     /locus_tag="VMZ53_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28927.1"
                     /translation="MAANVSVGSGVGKTLSPLRSAYVGVDVAGRLGDDPGAFISGSLS
                     YIDAKTFRFPFRAEARYGSAATCRCPDMDDQHARFTGVGLSVLMPTADMNIGGGSDRG
                     LNELGSDYRQFYGFGLGIDVDRVIGDWHATIVTLSMISDNFILRDREPY"
     gene            5656..6138
                     /locus_tag="VMZ53_10465"
     CDS             5656..6138
                     /locus_tag="VMZ53_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS28928.1"
                     /translation="MPIDSASYYATYGPMVLRRCRKLLGDDHAARDAMHDVFVQVLSR
                     SDSLVDQAPSSLLFRIATNVCLNRLRSRKRRPEDATPGLLDDIAAHTDPEAQTAARST
                     LATLFRQEPDNTALIAVLHLHDQMTLEEVAAEVGMSVSGVRKRLDKLRSKLRHLEVRP
                     "
     gene            6135..6920
                     /locus_tag="VMZ53_10470"
     CDS             6135..6920
                     /locus_tag="VMZ53_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28929.1"
                     /translation="MTTVPDWLVERIALDEVPAASKARVDAADARDIADRVAALRAAD
                     AAELAAYPAGPAIAQIEARVAREKQAAGRRRMRFIAGLGLAMATAAVLVVFVGGRTAP
                     VAGGESPGASEVTRVKGATRVAAFRQAGDKVEQLDEDSLVKAGDLIQLRYSAGGARFG
                     IIASVDGAGVVTLHHPAREDAPAEATALSTKTTALPNAYELDDAPDFERFFFITAEQP
                     IDVRGSIAALRAFAHRSDSATATLELPSGMHQTSLRLRKPSAP"
     gene            6917..8620
                     /locus_tag="VMZ53_10475"
     CDS             6917..8620
                     /locus_tag="VMZ53_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HUS28930.1"
                     /translation="MRTTMTTSMFLCCIASAIVAVVVGTYEPAHAKTSSEPALRRYAL
                     VIGSNTGGGAEREKLRYAGHDAATVADVLRQLGGVAQPDLSLLKEPDKRSLDDAFDAL
                     SKRVRDERKKGQRVELVVYYSGHADDAGILIGGARYDYARLRQRIRAVPADVHIAIVD
                     SCASGSFTRAKGGTKVAPFLQDASNQVEGFAFLSSSSEDEDAQESDRIGASFFTYFFV
                     AALRGAADRNRDGKITLNEAYQFSYEQTLGRTQNTSHGPQHPAYDMQLSGTGEVVITD
                     LRATDSALVLPTALKGRVTVVDASGRVSVEAAKEAGEPLSLALPNETYTVRVVNKDGE
                     FAATVTLEGETPFDPDSLQRVAPEKTIARGDFERGDDDDDDDDDRDPRRRHIPVGFGG
                     GLRVELPKTRTDFDALVTLGGTSRNVHGKPVIGVAETGDFAIGMTVGDNTHFAYDLGL
                     GVGPGVFIGDNVQVGATIGFGFSGITGGVLDFAWKLPTEAFVVLELSKDVRPMAYFRQ
                     SYIFSSEARQNGSKMARWGDESEAGAGVRFSGKLDGFMYGSVREMANERYWGVGLGAI
                     L"
     gene            complement(8617..9105)
                     /locus_tag="VMZ53_10480"
     CDS             complement(8617..9105)
                     /locus_tag="VMZ53_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HUS28931.1"
                     /translation="MKRLALMAVVLAACGGDDGGGDTKMASSTVSGFMGGTVTGTGMF
                     QQGAVDTAADSVTVTINLTGCVAGKAYPVHIHTGAACTDTTAQGGHWDMTRGEGIPNI
                     SCSGTSGSTVYNRAATDATLKWSVGDGSATDVIGHTIVVHDPDMPMTRIACGTITAAA
                     GS"
     gene            complement(9160..10368)
                     /locus_tag="VMZ53_10485"
     CDS             complement(9160..10368)
                     /locus_tag="VMZ53_10485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS28932.1"
                     /translation="MSIPGVLFLCVANSARSQMAEGLARAKFGDRLRVLSAGSRPTKV
                     NPFAIEAMAEAKLDITSHHSKLVDEIDPEGVELVVTLCAEEVCPTFLRPVRRLHWPIP
                     DPAMPAPVTSDLVEEDRYAREMRTRFRVARRTINSRLDALEAALALPQRTQIMPATAD
                     DRGEVEALLTACGLPLDGLDDVLGEMRPEQIASQRFVIARLDGELVGCAGIEQWEERG
                     LLRSVAVAEKARGTHIAQALVADRIAWAKSILTDYAPDGSETSKAFASISLLTTSAAG
                     FFEKLGFERVERTALPAPLLASTQLALPACSTAVAMTLTFFLTTEERLTRDIAAEIAQ
                     YGELMPPWAKHPEIPRRSIGWRMGGGEWYRWMWARWWDAQDKATRAAYRAKWVPPQEW
                     AGYLEPDDGH"
     gene            complement(10408..>10878)
                     /locus_tag="VMZ53_10490"
     CDS             complement(10408..>10878)
                     /locus_tag="VMZ53_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS28933.1"
                     /translation="LLGVEGGGFMMMMQKLQQERLAVAIGAVASMEQVLQDTIAYTKE
                     RKAFGKPISKFQNTQFKLVECLAKTEVSRAYVDKVVQEHVAGKQLVKECSIAKFWTTD
                     IAQEVIDTCLQFFGGYGYMLEYPVTRAFMDARVQRIYAGTNEIMKVIVAKQLGL"
BASE COUNT         1735 a         3717 c         3501 g         1925 t
ORIGIN      
        1 aaacgtctgg ttgccgacgt cgtcgagcga catcgcgaga tcgaagtcga acaggcacgc
       61 gacgttcgcg ccttcgtcga cgaggatgtt gcgcagcgtg aagtcgcgat ggatgagacg
      121 tgcggcgtgc agcgtcgcga ggccttcgag cgcttggacg agcagcttcg tgcgcgcgcg
      181 cggcgtcagg ttgtgcttgc cgatcgcttc gagcagcgtc atgcccatgc gcggcgtgac
      241 gaacagatag ttgtccgcga tcggcgccga gatgtacgtc ggcaggatgt tcgggtgctc
      301 gatcgccgca atgcgcgcca tcgccgcgag gttgttgtgg tgatcgcggc gcgtcacctc
      361 gtcggtgcgc agctgataga gatagttcga gtggaagacc ttgccgacga cggcctcttc
      421 gacgccgctc tcgtcatcgc gtcgttcgag cgtgtagaca gtccccatgc cgccgttggc
      481 gaggtgcgtg cccagcgttc gatagacgaa gcggttgtag cgaaacacca ggccgccctt
      541 ggccaggtcc tccgatcgca tcgtgccgat cgacgtggtt tcgtcccacg actcgctcat
      601 ccgccgtgtg gatgataacc cgctgaattc tcacggatcc agttccgggg cgacgtgttg
      661 tcgcattggc gtctggagct tcgaggatcg gcatcgaatg tggtacccac cctcgttgtc
      721 catgctcgaa caccgattcg ccaagctggc ggcgggcgca acgttcatcc tcctggtcat
      781 tggaggcacc gtgaatccca cgggttctag cctcgcgtgt ccggagccga cgctcgtctg
      841 ccacggccag ctgttcccgc cgatggtcgg cggcgtgctc tacgagcatg ggcatcggct
      901 cgcggcgatg acggtgggcc tgctgcagat tgcactgacg gtggccctct tccagagcag
      961 acgccggcgt cttgcggtgg gtctgctctg catgatcctc gcgcagggcg cgctcggcgc
     1021 gatcaccgtg aagtacaagc tgccgtggtt catctcgacg ttccacctgc tgctcggcat
     1081 gagctacttc gcgacactga tctacacggc gttcgcgacg cgtcccgcac cgagcgcgat
     1141 ggagctcgag cgccacgaga agctgcgcgc tgagctcggc acggcacgcc gttggatcgc
     1201 gatcgcgtgc ggcttcgtgt tcgtgcagct gctcctcggc gcactcgtcc gccacttcgg
     1261 cgctgcgatg gtttgcctcg gcgttccgtc gtgcacacgc gccggcgatt ggtggactgg
     1321 cctcgcgccc gttcagcacg ttcacatgat tcaccgcggg ttcggcgtta tcgtcgcgct
     1381 cgtgacgacg gtcgccgcca tcgccgtgta tcgccggacg aaggcgtggc cgcagctgcg
     1441 cctcctcgcg atgatcgcac cggtgctcgt gatcgcgcag gtcgcgctcg gcatctacac
     1501 cgtcctgacg atgcgctccg tgcccgttgc cgtcggtcac ttcgccggcg ccgcgagctt
     1561 gtgggcgctg tggatgagcg cgtggctgat gacggcgcgc cgtcagaagg ctgtcgcgat
     1621 tgcccccgag cgcgcgtcgg caacgaccga ggcggtgcct gcgtgaccgc aaaggctctc
     1681 accgcgaaga ccgtcgacct cgtcgcgctc gtcaagccga acatcatgat catggcgctg
     1741 ctcaccgccg ctggcgcgat gagccttgcg cccgggacgc tgccgcttgg caacgcgctg
     1801 tggcttctcg ccggcaccgc gctgatcgtc ggctcggcga atacgctcaa catgtggctc
     1861 gagcgggaca tcgactgcct gatgtcgcgc acgaagaacc gcccgctgcc gcaaggtcgc
     1921 ctgcacgcga cgaccgcgct cgtgttcggc gcagttcaag gcgccgtgtc gattccactg
     1981 ctcgcgatcg tcggaatcat tccggcggcg ctcggcctcg tcgcactgct gctctacgtc
     2041 ggcgcgtaca cgccgatgaa gcaagtctcg catcgctcgg tgtgggtcgg cggcgtgccc
     2101 ggcgcgatgc ccgcgctcat gggctggacg gctgcgacgg gtcatatcga tctcgccggc
     2161 ctcgccgtgt tcggcgtcct ctacatctgg cagatcccac acacgcacgc gatctcgatc
     2221 tatcgcgagc gcgagtatca cgcggccggg ctcaagacgc tgtcgggcac gcacggtgcc
     2281 acggtcgcgc gtcggtacat cctcgcgttc ctcgtcgtgc aggtcgccgc aagccttgcg
     2341 cccgcgctgc ttggcgtcgc cggtctgccg tatctcgtca ccgcgatcgc gctcggcgtc
     2401 atggtgctcg tgcaaggttt tgccggcaac ggcagcgcga agtgggcgcg caacgtcttc
     2461 ctgacgtcga tcgtctacct gccgatcttg ttcgcggtga tggtcgcgag cggcaacgcc
     2521 tagatggcga tgtccggtca cgagattcat cccgcgatca acgccgcgct caacctgacg
     2581 tcattcgtct tcctgctcct cggtcggtgg gagatcgcgc gcggcaacga agcggtgcat
     2641 cgccgtcgca tgctcacggc gtttacggcg tcgtgcgtgt tcctcgtctc gtatgtcatt
     2701 cgctttgcga cgacgggcac gcacaagtat cccggcaccg gctgggacaa gtatctctac
     2761 ctgttcattc tgttctcgca catggtgctc gcgatcgtgc tcgcgcccct gctgctcaag
     2821 gcgctgcgtc gcgcgcttgc tgctgacttc gtcgcgcaca aacggatcgt gaagtacacg
     2881 ttcccgatct gggtctacgt gtcagcgacc ggcgtgatcg tctacttgat gctctatcac
     2941 ctcgcgccgg cgcttcacgc gtagcatgcc gcggtgggtg gacgctggat tctgggggct
     3001 cgtctccgga tcggcgctcg tgctcggagc ttcggtcggc tacttcgtgc gggtaccggc
     3061 gcgcgtcatc gccgcgatta tggcgttcgg tgccggcgtg ctgatgtcgg cgctgtcgtt
     3121 cgatctgctc gacgccgcgt atctgcgagg aggcttcgat gcgaccgcga tcgggtttct
     3181 cgccggtgcc gcaatctaca ccgcagcgaa ctatgcgctc gcgcgtcgcg gcgcgaagca
     3241 ccgcaagcgg tccggcgacc aacagccttc ggatcggggg agcggcgcgg cgatcgcggt
     3301 cggcgcgctg ctcgatggaa tccccgagtc gatcgtgatc ggggtcagca tgatcggcgg
     3361 aggcgcggtc agcacggcgg ccgtcgcagc ggtctttctg tcgaacgtcc cggaaggcat
     3421 gtcgagcgcg gccggcatga agcgcgccgg gcgcgcccct ggctacgtct ttggcgtctg
     3481 gggcgcgatc gccgcagtct caggtgtctc ggcgctgctc ggctattcgg tgttcgccgg
     3541 gctgtccccg aacatcatcg cggcaacgac tgcgatcgcg gccggagcga ttctcgcgat
     3601 gctgtcggac acgatgatgc ccgaggcatt cgaggcggca cacgacctcg ccgggctcat
     3661 caccgtcgcc ggctttctct gcgcgtttgt cctgacgaag ctcggctgac tcaggtctcg
     3721 tagacgaggc cccagcggat gccgcgatcg cacgccacga agaccggcgc gctcgtgctc
     3781 ttcatgatgc acgcatagat cgctgcgccg cccgcgatca cgtctgcacg ctgcggctcc
     3841 atgccgggca tcgccttgcg ctcgacgacg gtggcgcgcg cgagccgctg atagaggccg
     3901 tcgacatcct cgggcgtgag ccgcaagccc gtcacttgat ccggatcgta cggcacgagc
     3961 tttagcgcga cggccgcgag cgtggtcgca gtccccgcgg tgccgatgat cgggagccca
     4021 gtggggaggc ccagtgcggc gagccggtcg tcgatgtcgc tcgcgagcgc ggcgagctcg
     4081 gctgcggacg gcgggtcggt ctttagatgg cgctcggtca gccgcaccgc gccgatcgga
     4141 atcgagaccg ccgagaccac cctcgcgccg tcgctgacaa tgaattcggt cgacccaccg
     4201 ccgacatcga cgacgatgaa cgcctgccct tccagctcgg gcatcgtctt cgcgaccgag
     4261 agaaatgcca gctcggcctc gcgcgcgccg ccgatcacct cgagcttcgc gcgcaggatc
     4321 tgctcggcag gaccgacgaa ctgcgccgca ttgcccgcct cgcgcagtgc ctgcgtgccg
     4381 atcacgcgcg gggtctcgac gccatggtcg tccatcacgg tgcggtactc gcgacagatg
     4441 tcgaggctct tcgcgatcga ctcggcagcc aagttcccgc tgccatcgag gcctttgccg
     4501 aggcggccga accggcagag atcgacgacc cgctgcttct gttcattcac gatcagcagc
     4561 aagagtgtgt tcgtgccgat gtcgatgacc gcgctgttca cgggttgacc ctactgcaat
     4621 gaccggcggc cgtcaggcgt ttcaatgtca gacacccaag cgagagttca ggccatggtt
     4681 acgatcacac acgtgcgctt cgatggcgaa ggcaccacgg cagagcacat cgtgatgctg
     4741 cgatggcgcc aagcgatcgg cgatacgaac atcacggaca tgatcagctg gatcgagtcc
     4801 ggcggcatcg ccaaggtgca gcgccccggc gaagaccgca tcgaggacgt caaagtcgtg
     4861 cgcgagagcg gccgcaagcc gtacctcgtg acgatcgccg acggccggcc gacgcagtcg
     4921 atcatcgagc tgccgcgctt ctagtacggc tcgcgatcac gaaggatgaa gttgtcggag
     4981 atcatgctga gcgtgacgat cgtcgcgtgc cagtcgccga tcacccgatc gacgtcgatg
     5041 cctaagccga atccgtagaa ctgccgatag tcggacccga gctcgttcaa accacggtcc
     5101 gatccgccgc cgatattcat gtcggctgtg ggcatcagca ccgacagtcc gacgccggtg
     5161 aaccgtgcat gctgatcgtc catgtccgga cacctgcacg tcgcggcgga cccgtagcgg
     5221 gcttcggcgc ggaacggaaa ccgaaacgtc tttgcatcga tgtagctgag cgagcccgag
     5281 atgaatgcac ccgggtcgtc gccaagccga ccggcgacat cgactcccac gtaggccgac
     5341 cgcaacggcg agagcgtctt gccgacgccg cttccgaccg aaacgttggc cgccacccga
     5401 ccgccgatgg tcgacctccc tgcgacactg ggtccaaact tcgagcgttg ggagatgcaa
     5461 ccggcagtca agagcacgag cacgatgagg agacgcacgc gccgcatcta agcgggtgcc
     5521 atggcgcgcc gcgatggcga cgcagcccgt tgccgttcgt tgcgcgccgt tgcccatgac
     5581 tgcccgtacg cgccgcgaaa aatagcgggg gcacttcctg ccagctgcgg tgttacaccc
     5641 tcgtccgcca gcaccgtgcc catcgattcc gccagttact acgcgacgta cggcccgatg
     5701 gttctccggc gctgccgcaa gctcttgggc gatgaccacg ccgcacgcga tgccatgcac
     5761 gacgtctttg tccaagtcct cagccgctcc gacagcctgg tcgatcaggc gccatcgtcg
     5821 ctgctgttcc gcatcgcgac gaacgtctgc ctgaatcgat tgcggagccg gaagcgacgt
     5881 ccggaggacg cgacgcccgg gctgctcgac gacatcgccg cgcacaccga tccggaggcg
     5941 cagaccgctg cgcgctcgac gctcgccacg ctgtttcgcc aagagcccga caacaccgcc
     6001 ctcattgccg ttctgcatct gcatgatcaa atgacgctcg aagaggtcgc ggcggaagtc
     6061 ggcatgtccg tctcgggcgt gcgcaagcgg ctcgacaagc tccgctccaa gctccgtcac
     6121 ctggaggttc gcccatgact accgtgcccg actggctcgt cgaacggatc gcgctcgacg
     6181 aagttcccgc ggcaagcaaa gcccgcgtcg atgcggcaga cgcacgcgac atcgctgatc
     6241 gcgtcgctgc tctacgcgcc gccgatgccg ccgagctagc tgcctaccct gctggccccg
     6301 ccatcgcgca aatcgaagca cgcgtcgcgc gtgagaagca ggccgccggc cgtcgccgca
     6361 tgcgcttcat cgccgggctt ggtctcgcga tggcgaccgc cgccgtgctc gtcgtcttcg
     6421 tcggcgggcg gacggcaccc gttgcaggcg gtgagtcccc aggcgccagc gaagtcacac
     6481 gcgtcaaagg cgcgacccgc gttgcggcct tccgccaggc gggcgacaag gtcgagcagc
     6541 tcgacgaaga ctccctcgtg aaggccggcg acttgatcca gctgcgctac agcgccggtg
     6601 gcgcgcgatt cggcatcatc gcgtcggtcg acggagcagg cgttgtcacg ctgcatcatc
     6661 ccgcgcgcga ggatgcaccc gccgaagcca ccgcgctctc gacgaagacg accgcgctgc
     6721 cgaatgccta cgagctcgac gacgcgcccg acttcgagcg gttcttcttc atcactgccg
     6781 aacagccgat cgatgttcgc ggcagcatcg cggcgcttcg cgcgttcgcg caccgctccg
     6841 acagcgcgac cgctacgctc gagctgccga gcggcatgca ccaaacctct cttcgcctgc
     6901 gcaaaccgtc tgctccatga ggaccaccat gactacctcg atgtttctgt gctgcatcgc
     6961 ctcggcgatc gtcgctgtcg tggtcggcac gtacgagcct gcgcacgcaa agacgagcag
     7021 cgagcccgcg ctccgtcgct acgcgctcgt catcggtagc aacaccggtg gcggcgccga
     7081 gcgcgagaag ctgcgctatg cagggcacga cgcggcaacg gtcgcagacg tgttgcgcca
     7141 gctcggcggt gtcgcgcagc cggatctctc gctgctgaag gagcccgaca agcgctcgct
     7201 cgacgatgcg ttcgacgcac tctcgaagcg tgtacgcgac gagcgcaaga agggccagcg
     7261 cgtcgagctc gtcgtctact actcgggcca cgccgacgat gccggcattc tgatcggcgg
     7321 cgcgcgctac gactacgcgc ggttgcgcca gcgcattcgt gccgtgcccg cagacgttca
     7381 catcgcgatc gtcgacagct gcgcctcggg cagcttcacg cgcgcgaagg gcggcacgaa
     7441 ggtcgcgccc tttctgcaag acgcgtcgaa tcaggtcgag ggcttcgcgt tcctgtcatc
     7501 gagctccgag gacgaagacg cgcaggagtc cgatcgcatc ggcgcgtcgt tcttcacgta
     7561 cttcttcgtc gctgcgctgc gcggtgccgc cgatcgcaac cgcgacggca agatcacgct
     7621 caacgaggcg taccagttct cgtacgagca gacgctcggc cgcacgcaga acacgtcaca
     7681 cggcccgcag catcccgcgt acgacatgca gctgtcaggc accggcgagg tcgtgatcac
     7741 cgatctgcgc gcgaccgact cggcgctcgt gctgccgacc gcgctcaagg gccgcgtcac
     7801 cgtcgtcgat gcgagtgggc gcgtgtccgt cgaagctgcc aaggaagccg gcgagccgct
     7861 ctcgctcgcg ctgccgaacg agacgtacac ggtgcgcgtc gtcaacaagg acggcgagtt
     7921 cgcggcgacc gtgacactcg aaggcgagac gccgttcgat ccggacagcc tgcagcgcgt
     7981 tgcaccggag aagacgatcg cacgcggcga cttcgagcgc ggcgacgacg atgacgatga
     8041 cgatgacgat cgcgatccgc gacgtcgtca catcccggtc ggcttcggtg gcggcctccg
     8101 cgtcgagctt ccgaaaacgc gcaccgactt cgacgcgctc gtcacactcg gcggcacatc
     8161 gcgcaacgtc cacggcaagc ccgtcatcgg tgtcgcggag accggcgact tcgcgatcgg
     8221 catgaccgtc ggcgacaaca cgcacttcgc gtatgacctc ggccttggcg tcggtcccgg
     8281 cgtgttcatc ggcgacaacg tgcaggtcgg cgccacgatc ggatttggct tctccggcat
     8341 taccggcggc gtcctcgact tcgcgtggaa gctgccgacc gaagcgttcg tcgtcctcga
     8401 gctctcgaaa gacgtgcgcc cgatggcgta cttccgccag agctacatct tcagctccga
     8461 agcacgccag aacggctcga agatggcgcg ctggggcgac gaatcagagg ccggcgccgg
     8521 cgtccgcttc agcggcaagc tcgacggctt catgtacggc agcgttcgcg agatggcgaa
     8581 cgagcgctac tggggcgtcg gcctcggcgc gattctctag ctgccggcag cggcggtgat
     8641 cgtgccgcac gcaatgcgcg tcatcggcat gtccggatcg tgaacgacga tcgtgtgacc
     8701 gatgacatcg gttgccgagc catcaccgac gctccacttt agcgtcgcgt ccgtcgctgc
     8761 gcggttgtag accgtcgacc cgctcgtgcc actgcacgag atgttcggga tgccctcgcc
     8821 gcgcgtcatg tcccagtgac caccctgcgc cgtcgtatcg gtgcacgccg cgcccgtatg
     8881 gatgtggacg ggatacgcct tcccggccac gcagccggtc aagttgatcg tgaccgtcac
     8941 gctgtcggcg gcggtgtcca ccgcgccctg ctggaacatg cccgtgccag tcacggtgcc
     9001 ccccatgaag ccgctgacgg tcgacgacgc catcttggtg tcaccgccac cgtcgtcacc
     9061 accacacgcc gcgagcacaa ccgccatcaa tgccagtctc ttcatctgtc cctccaatgt
     9121 gagcgcgcac cataacgcag cgctcgtgag ggagcgactt cagtggccgt cgtcgggctc
     9181 cagatagcca gcccattcct gcggcggcac ccacttcgcg cgatacgcgg cgcgcgtcgc
     9241 cttgtcctgc gcgtcccacc agcgggccca catccagcgg taccactcgc cgccgcccat
     9301 tcgccaaccg atcgatcggc gcgggatctc cgggtgcttc gcccatggcg gcatcagctc
     9361 gccgtattgc gcgatctcgg ccgcgatgtc gcgcgtgagg cgttcttcgg tcgtcagaaa
     9421 gaacgtcagc gtcatcgcga cggcggtcga gcacgcaggg agcgcgagct gcgtcgaggc
     9481 gagtagcggc gcgggcaacg cggtgcgctc gacgcgctcg aacccgagct tctcgaagaa
     9541 gccggcggcg ctcgtggtga gcagcgagat cgaagcgaac gctttgctcg tctcgctgcc
     9601 gtcgggcgcg taatccgtca ggatcgactt tgcccacgcg atgcgatcgg cgacgagtgc
     9661 ctgcgcgatg tgcgtgccac gagccttctc cgcgaccgcc acggagcgga gcaagccgcg
     9721 ctcttcccac tgctcgatgc cagcgcagcc gacgagctca ccatcgagcc gcgcgatcac
     9781 gaaccgctgt gaagcaatct gctcgggacg catctcgccg agcacgtcgt cgaggccatc
     9841 gagcggcagc ccgcaggcgg taagcagcgc ttcgacctcg ccgcgatcgt cggccgtcgc
     9901 cggcatgatc tgcgttcgct gcggcaacgc gagcgccgct tccagcgcat cgaggcgcga
     9961 gttgatcgtc cgccgtgcca cgcgaaaccg cgtccgcatc tcgcgcgcgt agcgatcttc
    10021 ctccacgaga tccgacgtga cgggcgctgg catcgccggg tccggaatcg gccagtgcag
    10081 gcggcggacg ggtcgcagaa acgtcgggca gacttcctcg gcacacagcg tgacgaccag
    10141 ctcgacaccc tctggatcga tctcgtcaac gagcttgctg tgatgcgacg tgatgtcgag
    10201 cttggcctcc gccatcgcct cgatcgcgaa tggattgacc ttcgtcggcc gcgagcccgc
    10261 gctcagcacg cgcaagcgat cgccgaattt tgcgcgagcg aggccttcgg ccatctggct
    10321 gcgcgcggag ttcgcgacgc acaggaacag cacgcctggt atcgacacga accgaggcta
    10381 caccacgact gtggcgacga cgtgacgcta gaggccgagc tgcttcgcga cgattacttt
    10441 catgatctcg ttcgtgccgg cgtagatgcg ctgcacgcgt gcatccatga acgcgcgcgt
    10501 caccgggtac tcgagcatgt agccgtagcc gccgaagaac tgcaggcacg tgtcgatcac
    10561 ttcttgcgcg atgtctgtcg tccagaactt cgcgatcgag cactccttga cgagttgctt
    10621 gccggcgacg tgctcctgca cgaccttgtc gacgtacgcg cggctgactt ctgtcttcgc
    10681 gaggcactcg acgagcttga actgcgtgtt ctggaacttg ctgatcggct tgccgaacgc
    10741 tttgcgctct ttcgtgtacg cgatcgtgtc ttgcagcacc tgctccatcg acgcgactgc
    10801 accgatcgcg accgcgagcc gctcttgctg cagcttctgc atcatcatca tgaagccgcc
    10861 gccctccaca ccgaggag
//
LOCUS       DATLBZ010000201        11370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_199330, whole genome shotgun sequence.
ACCESSION   DATLBZ010000201 DATLBZ010000000
VERSION     DATLBZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199330"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VMZ53_10495"
     CDS             complement(<1..626)
                     /locus_tag="VMZ53_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28934.1"
                     /translation="MTVGVVGVCARAHADDIPIVTLTSSGPDAEIPTNRAFYIAGDAS
                     DTSEFVQVVVVRRGNPSMFGDDGASCHDVLADLQIDAVTSTAADDDGGSGEGGELTVD
                     PMLQPRYDAGVHRAYEIFPHASGSARRSAVLVTTAWQRSSAGPTQFRVLVPHERDFFA
                     PGYGYCLMAVTTAHAQVIGDDAIEDLVDELASKIVACGDSTKCDDDALA"
     gene            867..1688
                     /locus_tag="VMZ53_10500"
     CDS             867..1688
                     /locus_tag="VMZ53_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS28935.1"
                     /translation="MRARKQEREIGRGRSSVGAPQTPIAPCELPSAASGLLERTRVFQ
                     TKKRTFVFRLIGRGTPPYDCGVPRHARIEKTKPSVERILEAAEGLFASNAYGDISLRQ
                     LIAAAGISTTAFYARFGSKEAVLDKLTESLFVGLQVEAATTLRSVKNLDEGIERGVDV
                     LCARFAPRKSLVRLVIAESGSIGPTSSMRRKSYVMLVGFMAHYFKALAGRGRIEIGNA
                     RDGEAEAQAEALAWALVGALEMQIVRWAVWDELDIDELRAALIAVARVILPKERV"
     gene            1709..3877
                     /locus_tag="VMZ53_10505"
     CDS             1709..3877
                     /locus_tag="VMZ53_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28936.1"
                     /translation="MLAVVSGVTACGDDRTTVVMTAADEAPAFGAAPYPSDALREGDH
                     LGVIRGLDAIVDRHSELIAAQLAALDGFGLRPLVEFPLTGGLDAESVPATTRAIGDAA
                     GLVDVDPASAERGRVIAMEWHYDDARRVLQGAPMSGEVLREGTRYAAFVTSRVTAADG
                     EPLAKAEAFDELHGARWASTAEALRELGALNKDIVGVTAFTTQHATAPMIAAKAIAAA
                     LPPSELRFDDPAIIFSGTTALDRVIGVATRATDGPRAGLERWGNDNPTGIAHDHVGVI
                     GSGTMTIARFRRDDTASDGPEDETFENPVRVIAMDTIPVTFIVPSSPMPASGYPVIIY
                     GHGLGASRDQLLSFAEPLTAQGYVIAGIDMSGHGSRFDPSDVHANMANQLPSFSGDGA
                     VRDGFGDTTGLTTQFDFFEGFLGVGGVRDSIRQSALDLGRLAQMLRRPDLDLSALAGP
                     GSASPKLDTTRIAYLGESFGTVVGTVFAATEPNVDLFVLDVPGGGILDHLLPESAEIG
                     SLAMPIIESIYGPRDRLSRWHPLIGMMQAVVDGADPLSYAPHILDDRFDVPGIGVLRP
                     RSIVCLEVVGDQVLSNTGTDALARELKLDVLTPDLAPPSGLVEIASPAAGNRAGQTAI
                     LVQYAPASHGANWSSEKGVIRYMPGFPQEGDTPFPRLPAPITIRQPIYETHAQVAEIL
                     QTYFAGEMPRVRSTLAPIADFDADGAPDASDAAPYDPTVH"
     gene            complement(3874..4671)
                     /locus_tag="VMZ53_10510"
     CDS             complement(3874..4671)
                     /locus_tag="VMZ53_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28937.1"
                     /translation="MPWVLLGCDETFTPASQIREIHDGPSTAERGAMHRYLLFLPLCA
                     GCLDSSLPKMDALVSSSHVSASAEVSYRRCDGEPCTYVELELFRDEHYASDTSAQFYI
                     DYAQPMRPKFEPNGGDLPGGRYTMTVHGWIEHVRINANVGSDSVYMEASRAISNPDEA
                     NIVLPYEVVLGARPTLQWDGQGSPYSYNLLYATQVPALRFGFLATLAADNGAFEFDRT
                     TFTAAGSHTVALYRQVDYEATDDDVNAMLVWSSEWQQIVQVVEAPNG"
     gene            complement(4700..5461)
                     /locus_tag="VMZ53_10515"
     CDS             complement(4700..5461)
                     /locus_tag="VMZ53_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28938.1"
                     /translation="MGRDTRESAAELRAIAPLVVTGPQLQLVGPCTLVTNGSQTIAFS
                     SAELLRQAGEPLAIALTLDCTKTLPVASWSMGRVPNMGIIELGVPFPFKDGKIDVAPL
                     SLGAVCATVDTRGAPSALVSVQRASDGRGVMRRVIPVHVDAVDGGGISDDVITRLASP
                     DNADDVGVDVDGAVLISWMPADPVLGRPSEVVAVALSVPYRRKAFKPRELPAIAELFG
                     LDDLGRALPWEAARKDDASNELGQIAGEIRPDAKK"
     gene            5584..6141
                     /locus_tag="VMZ53_10520"
     CDS             5584..6141
                     /locus_tag="VMZ53_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUS28939.1"
                     /translation="MTRAKVRRYRIVVGVDLSDYSQIVIEQALDQAARHQSPDLHFIH
                     VAERRKDSIEELSERLSAAVYPSLQVFNQYGTDWRARLHVRRGKPEQQINILAADILA
                     DLIVVGQFGLHRRSTANRVLTHAHCATLVVGMPKEVDLSQCHACSATREDSDGALWFC
                     DEHVVGTAAVSPQSVWTNGNGTLMR"
     gene            6166..7434
                     /locus_tag="VMZ53_10525"
     CDS             6166..7434
                     /locus_tag="VMZ53_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HUS28940.1"
                     /translation="MLRRALVATFRGIIRLYFRQIERVGEPPGPDTRGRVFVSNHHNA
                     LIDPVLVLTDAQCEISPIAKSTLWKIPGLKYLLDGAGAVPLYRRKDDPNRAADANDAV
                     FGKIAEHLSHGGNILIFPEGTSHSQPHLAPLRTGAARMLVAGEKQGGTLTFQAVALEF
                     DEREQFRSRCLVLWGPVRRLADVAGEGDERVRAITAQMEADLSELLIGGDTHEERRLV
                     ARVAEMFANDAGDASLVGWSTIGRQVEAAGNMLRSAEPELVAHVRTKIETYYAELERL
                     GRTDAQTSGAAAATPKSPTRWLRRIALAPLAVPGFVLYGLPYFIPRLVARSSDADAVS
                     TVKLGTALVVYPVWAATLVGLSLAFIPPPLSLFAAAIVIASPFAALHWLDAYWNRTPD
                     RELTPDELATLARLRIAARTAIGEARTRLG"
     gene            7473..8327
                     /locus_tag="VMZ53_10530"
     CDS             7473..8327
                     /locus_tag="VMZ53_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28941.1"
                     /translation="MHALIVVAALATDWFDGLTAKVIEDVKAGKPLVVEVHVPLCDNE
                     FTRCGNAKLGDGDNPETNLYWSTTPGLGVWFARKAGGWQRVFHATDGKDTGDKDVLEL
                     HVYRRTITTSAAWRKLGAPKSYEVDLVVHGWRGSAIDRALAAYASDVSGEGTRTITLA
                     NKTEIAAGGAARIVAFVGHNRLMDLDKFEWPKPGADIKGTIAIACHTKSYMKEDVPAA
                     SRVPLLMTNDFLFSNAAPLEAAVLAFARGGGYAAIRKDAATAYAGVQKRDVKRVIGAF
                     TNPADKKW"
     gene            complement(8328..9074)
                     /locus_tag="VMZ53_10535"
     CDS             complement(8328..9074)
                     /locus_tag="VMZ53_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS28942.1"
                     /translation="MRSAVALVLVTGCRSVLGLEPPAQQVVDDAPRVDRDTAMPDGAP
                     FAACGNDMNLVACFDFEDTVKDLAPSPNSVAGVNVAYSSGQRGKAALLDTASRIAIQD
                     SVLLDVAAVSIEAWIYPTQTPSPAGRMGVIDVNGQYGMFLKPASAGAVLVCTAGSTFE
                     AGGIAINQWTHVACTSANSVSIMYINGQVAAVATFPTPLETSPSDGGEVGGNSPDGLD
                     RFIGKIDVLRVYRTKRTDAEICADAAACAQ"
     gene            complement(9091..9633)
                     /locus_tag="VMZ53_10540"
     CDS             complement(9091..9633)
                     /locus_tag="VMZ53_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28943.1"
                     /translation="MSYRQLPTASRRIGFEIQTPTEVEVQAGDVLTCRERRASGETIG
                     ELEVSVFHAALVIDRDGILEEKMEAAVDSNSEPGSRVLPPIPVALPGASGYRADIEVV
                     RPMGHARPALPYVYVFAVAPNDLALDGGLIVTVRSASPEWPAADKILRSLKILSRRGV
                     TANDTDDAPPMLPLLGPRDD"
     gene            complement(9682..10293)
                     /locus_tag="VMZ53_10545"
     CDS             complement(9682..10293)
                     /locus_tag="VMZ53_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HUS28944.1"
                     /translation="MERQLDLLGRGAPSFDREFRTLERRELSHGAWVDHVPSWVSGHD
                     ALFDELEGSLQWRRETMKMYDKTIEVPRLLARVPREGIVDEMRAAISAKYGEDFVFTT
                     AALYRDGNDSVAFHGDTTARNMVEALVATVSLGASRRFMLRPAEGGPSIALPLGRGDL
                     VVMGGTCQRTWRHGIPKVASAGPRIALMFRPKWAKNYRTSVDD"
     gene            10310..10783
                     /locus_tag="VMZ53_10550"
     CDS             10310..10783
                     /locus_tag="VMZ53_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28945.1"
                     /translation="MGEAKGGRDRKTTTKMRKIAAPAGYSGTPLPQKLGIKEDHEVAV
                     LDAPRGFFDTLGKLPSGATVQTRLAGKMPLDVVVAFATKVADLTRAIKNVRPRMQPAA
                     GLWIAWPKKSSGVPTEITEDVIRAIALPTGLVDNKVCAIDETWSGLRLVIRKENR"
     gene            complement(10761..>11370)
                     /locus_tag="VMZ53_10555"
     CDS             complement(10761..>11370)
                     /locus_tag="VMZ53_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HUS28946.1"
                     /translation="EAAGAAAPKRFAGHTYWGRAITGFGDPKARLLVVGLAPAAHGGN
                     RTGRVFTGDKSGDWLYGALHRAGFANQPHSTELDDGLRLTDAYVSVVVRCAPPGNKPT
                     PIERDKCLPFLVRELEALPKVKAIIALGAFAWDGVLRAARELGHPLPTPRPTFGHGAR
                     STLGPYSLLGSYHVSQQNTFTGRLTEPMLDAVFRAASDSLCG"
BASE COUNT         1932 a         3727 c         3785 g         1926 t
ORIGIN      
        1 gcgagcgcgt cgtcgtcgca tttcgtcgag tcaccgcacg cgacgatctt gctcgccagc
       61 tcgtcgacca aatcttcgat cgcgtcgtcg ccaatcacct gcgcgtgcgc agtcgtcacg
      121 gccatcaagc agtagccata ccccggcgca aagaagtcgc gctcgtgcgg cacgaggacg
      181 cgaaactgcg tcggccccgc gctcgaccgc tgccacgccg tcgtgaccaa caccgccgat
      241 cgcctcgcgc tgccactcgc atgcggaaag atctcgtacg cccgatgcac gccggcgtca
      301 taacgaggct gcagcatcgg atcgacggtg agctcaccgc cctcgccgct gccgccatcg
      361 tcatcagcgg ccgtcgatgt cacggcatcg atctgcagat cggcgagcac gtcatgacaa
      421 ctcgcgccgt cgtcgccgaa catgctcgga ttgccgcgcc gaacgacgac gacctgcacg
      481 aactcgctcg tgtcgctcgc atcgccggcg atatagaacg cccgattcgt cggaatctcc
      541 gcgtcggggc ccgagctcgt cagcgttacg atcggaatgt cgtccgcatg cgctcgcgcg
      601 caaacgccga cgacgccaac ggtcaacgtt gtggtccaca acagtacgag gcagcgctgc
      661 acgtcttccc ccagcccgaa atcgtaaacc ggccgcgccc gccgcgcctc attccccgcg
      721 cccgcggacg atagcgcccg ccgcgcccgc ggacgatagc gctcgccgcc ccgcggacga
      781 tagcgcccgc cgcgcccgcg gacgatagcg cccgccgccc gcggacgata gcgcccgccg
      841 cgcccgccgc cggatagcgc aggcgagtgc gagctcgaaa acaagagcgc gaaatcgggc
      901 gaggccggag cagcgttggc gcgcctcaaa cgcccatcgc accttgcgag ctgccttctg
      961 ccgcttcggg actccttgaa agaacgaggg ttttccaaac taaaaaacga acgtttgttt
     1021 ttcgattgat cgggcgaggc accccaccgt atgactgcgg ggtgccgagg catgcgcgga
     1081 tcgagaagac gaagcccagc gtcgagcgca ttctcgaggc ggccgagggg ctgtttgcgt
     1141 cgaacgcgta tggcgacatc tcgctgcggc agctgatcgc tgcggctggg atttcgacga
     1201 ccgcgtttta tgcgcggttt ggttcgaagg aggccgtcct cgacaagctg acggagtcgc
     1261 tctttgtcgg cttgcaagtc gaggccgcga cgaccttgcg cagcgtgaag aatttggacg
     1321 agggcatcga gcggggtgtc gacgtcttgt gcgcgcgctt tgcgccgcga aagtcgctcg
     1381 tgcggttggt gatcgcggag tcggggtcta tcgggccgac gtcgtcgatg cggcggaagt
     1441 cgtacgtgat gttggtcggg ttcatggcgc attacttcaa ggcactcgcg gggcgcgggc
     1501 ggatcgagat cggtaacgcg agggatggag aggcggaggc gcaggcggag gcgttggcgt
     1561 gggcgcttgt tggagcgctc gagatgcaga tcgtgcgatg ggcggtgtgg gacgagctcg
     1621 atatcgatga gctgcgtgcg gcgttgattg cagtggcgcg ggtgattttg ccgaaggagc
     1681 gtgtgtgatg cgtaagctgt cgtgttttgt gctggcggtg gtttcgggtg tgacggcgtg
     1741 cggcgacgat cggacgaccg tcgtgatgac agcggcggat gaagcgccgg cctttggggc
     1801 agcgccgtat ccgtccgatg ccctgcgcga gggtgaccac ctcggcgtga ttcgcggcct
     1861 cgatgcgatc gtcgatcggc attcggagct cattgctgcg cagctcgcgg cactcgatgg
     1921 attcgggctg agaccgctcg tcgagtttcc gctgaccggc gggctcgatg cggagagcgt
     1981 tccggcgacg acgcgcgcga ttggcgatgc ggccggactg gtcgacgtcg atccggcgtc
     2041 ggcggagcgt ggccgcgtga tcgcgatgga gtggcactac gacgatgcgc ggcgcgtgtt
     2101 gcaaggcgcg ccgatgagcg gcgaagtgct gcgcgaaggc acgcggtatg cggcgttcgt
     2161 gacatcgaga gtgaccgctg ccgacggcga gccactcgca aaggccgagg cgttcgacga
     2221 gctgcatggc gcgcggtggg catcgaccgc ggaggcgctg cgcgagctcg gcgcgctgaa
     2281 taaggacatc gtcggcgtta ccgccttcac gacgcagcac gcgaccgcac ccatgatcgc
     2341 cgccaaagcg atcgcggcgg cgctgccgcc atcggagctg cgcttcgacg atcccgcgat
     2401 tatcttttcg gggacgaccg cactcgatcg cgtgatcggc gtcgcgacgc gagcgacgga
     2461 tggaccgcgg gccggcctcg agcggtgggg caacgacaat ccgaccggga tcgcgcacga
     2521 tcatgttggt gtgatcggga gcggcacgat gacgatcgcg cggtttcgac gcgatgacac
     2581 tgcgagcgat ggccccgagg acgagacgtt cgagaatccg gtgcgcgtga tcgcgatgga
     2641 tacgattcct gtcacgttca tcgtcccgtc gtcgcccatg ccggcgtccg ggtatcccgt
     2701 catcatctat ggtcacgggc ttggtgcgag ccgcgatcag ttgctgtcgt ttgcagagcc
     2761 gctgacggcg cagggctatg tgatcgccgg catcgacatg agcgggcatg gctcgcgctt
     2821 cgatccgagc gacgtgcacg cgaacatggc gaaccagctg ccgtcgttct cgggtgacgg
     2881 cgcggtgcgc gacgggtttg gcgacacgac cgggctcacg acgcagttcg atttcttcga
     2941 gggcttcttg ggcgtcggcg gcgtgcgcga ctcgattcgc cagtcggcgc tcgacctcgg
     3001 gcggctcgcg cagatgttgc ggcggccgga tctggatctg tccgcactcg ccggaccggg
     3061 cagcgcgagc ccgaagctcg atacgacgcg catcgcgtac ctcggcgagt cgttcggcac
     3121 cgtcgtcggg acggtatttg ccgcgaccga gccgaacgtc gaccttttcg tcctcgacgt
     3181 gccgggcggc ggcatcctcg atcatctgct gccggagagc gccgagatcg gatcgctcgc
     3241 gatgccgatc atcgagagca tctatggacc gcgcgatcgg ctctcgcgat ggcatccgct
     3301 catcggcatg atgcaggcgg ttgtcgacgg cgccgatcca ctgagctatg cgccgcacat
     3361 cctcgacgat cgcttcgacg ttccgggcat cggcgttctg cggccgcgca gcatcgtctg
     3421 tctcgaggtc gtcggcgatc aggtgctgtc gaacaccggc accgatgcgt tggcgcgcga
     3481 gctgaagctc gatgtgctga cgccggatct cgcgccgccg agcgggctcg tcgagatcgc
     3541 gtcgccggct gcgggcaatc gcgcagggca gacagcgatc ctcgtgcagt acgcaccggc
     3601 gagccacggc gcgaactgga gcagcgagaa gggcgtcatt cgctacatgc ccggtttccc
     3661 gcaggagggc gacacgccgt tcccgcgcct ccctgcgccc atcacgatcc gccagccgat
     3721 ctacgagacg cacgcgcagg tcgccgagat tttgcagacg tactttgcgg gcgagatgcc
     3781 acgcgttcgc agcacgctcg caccgatcgc ggacttcgat gccgacggcg cgcccgacgc
     3841 gagcgacgcc gcgccctacg atccgacggt gcactagccg ttcggcgctt cgacgacctg
     3901 tacgatctgt tgccactccg agctccacac gagcatggcg ttgacgtcgt cgtcggtggc
     3961 ttcgtagtcg acttggcgat agagcgcgac ggtgtgggaa ccggcggcgg tgaacgtggt
     4021 ccgatcaaac tcgaacgcgc cgttgtccgc tgcgagtgtc gcgagaaatc caaagcggag
     4081 cgccggcacc tgcgtcgcgt acagcaagtt gtagctgtac gggctcccct gtccgtccca
     4141 ctgcagcgtt gggcgcgccc cgagcacgac ctcgtacggc agcacgatgt tggcttcatc
     4201 ggggttcgaa atcgcgcgcg acgcttccat gtagacgctg tcgcttccga cattcgcgtt
     4261 gatgcgcacg tgctcgatcc atccgtgcac tgtcatcgtg taacgaccgc cgggaagatc
     4321 acctccgttc ggctcgaact tcggcctcat cggctgtgcg tagtcgatgt agaactgcgc
     4381 gctcgtatcc gaggcgtagt gttcatcgcg aaatagctcg agctcgacgt acgtacacgg
     4441 ctcgccgtca catcgccggt agctcacttc cgcgcttgct gatacgtgcg acgacgagac
     4501 aagcgcatcc atcttcggca ggctcgaatc gagacaacca gcgcacagtg ggaggaagag
     4561 caggtatcga tgcatcgctc cccgctctgc agtcgatgga ccatcgtgaa tctcgcgaat
     4621 ttgcgaggct ggcgtgaacg tctcgtcaca gcctagtaga acccacggca cgatgaacga
     4681 gccttagccc ggtcgccggc tacttctttg cgtcggggcg aatctcgccc gcgatctggc
     4741 cgagctcgtt gctggcgtcg tctttgcggg cggcttccca cggaagagca cgtcctaggt
     4801 catcgagtcc gaacagctcg gcgatcgccg gcagctcgcg cggcttgaac gccttgcgcc
     4861 gatacgggac tgaaagtgcc acggcgacga cctcgctcgg acgaccgagc actggatccg
     4921 ccggcatcca cgagatgagc acggcaccat cgacatcgac accgacgtcg tcggcgttat
     4981 cgggactcgc gagtcgcgtg atgacgtcgt cgctgatgcc accgccatcg acggcatcga
     5041 catggactgg gatcacgcga cgcatgacgc cgcgtccatc acttgcgcgc tgcacgctga
     5101 cgagcgcgga cggagcgccg cgcgtatcaa ccgtcgcgca gacggcgccg agcgagagag
     5161 gcgcgacatc gatcttgcca tccttgaatg ggaacggcac gccgagctcg atgatgccca
     5221 tgttcgggac gcggcccatc gaccacgatg cgaccggcag cgtcttcgtg cagtccagcg
     5281 tgagcgcgat cgcgagaggc tcacctgcct gtcgcagcag ctccgcgctc gagaacgcga
     5341 tcgtctgaga gccattcgtg accagcgtgc acggaccaac gagctgcagc tgtgggcccg
     5401 tcacgacgag cggtgcgatg gcgcgcagct cggcggccga ttctcgcgtg tctcgcccca
     5461 cggggggaga ctggcacggc tagccagtgt gtctcaactg gcaatggtgc gtcgcagccc
     5521 gtcgcgcagc gtgcgcctac gcccaccgac gatgcgggca cgcggactgc actgatcggc
     5581 gccatgactc gcgccaaggt ccgtcgttat cgcatcgtcg ttggagtgga tctgtcggac
     5641 tactcgcaga tcgtgatcga gcaagcactc gatcaagcag ctcggcatca atcgccggac
     5701 ttgcacttca ttcacgtggc ggagcgtcgc aaagactcga tcgaggagct cagtgagcgg
     5761 ttgtccgcgg ctgtgtatcc gtcgctccag gtcttcaacc aatacggcac tgactggcgc
     5821 gctcgcctgc atgtgcgtcg tggcaagccc gagcagcaga tcaacatcct cgcggccgac
     5881 atcctcgcgg acctgatcgt cgttggtcag ttcggtctgc atcgccgcag caccgcgaac
     5941 cgcgtgctca cccacgcgca ctgcgcgacg ctcgtcgtcg gtatgccgaa ggaagtcgac
     6001 ctgtcgcagt gccacgcctg ctcggcaacg cgcgaggatt ccgacggcgc gctgtggttc
     6061 tgtgacgagc acgtcgttgg caccgccgcg gtttcgccgc agagcgtgtg gacgaacggc
     6121 aacggcacct tgatgcgctg agcctgtcgg tggcatcgtg gatggatgct gagacgcgcg
     6181 ctcgtcgcga cgtttcgcgg aatcatccgc ctctacttcc gtcagatcga acgcgtcggt
     6241 gagccgcccg gtcccgatac gcgcggtcgc gtgttcgtga gcaatcacca caacgcgctg
     6301 atcgatccgg tcctcgtgct caccgacgcg cagtgtgaga tttcgccgat cgcgaagtcg
     6361 acgctgtgga agattcccgg cctcaagtac ctgctcgatg gcgcgggcgc ggtgccgctg
     6421 taccgtcgca aggacgatcc gaatcgcgcg gcagacgcaa acgacgccgt gttcggaaag
     6481 atcgccgagc acctctcgca cggcggcaac atcctcatct ttcccgaagg cacgagtcac
     6541 agccagccgc acctcgcccc acttcgcaca ggtgctgcgc gcatgctcgt cgccggcgag
     6601 aagcagggcg gcacgctgac atttcaggcg gtcgctctcg agttcgacga gcgcgagcaa
     6661 ttccgttcgc gctgcctcgt gctgtggggc ccggtgcgac gtctcgctga cgttgcgggc
     6721 gaaggcgatg aacgcgtgcg cgcgatcacg gcgcagatgg aagcagatct cagtgagctg
     6781 ctcatcggcg gggacacgca cgaggagcgg cgcctcgtcg cacgcgttgc ggagatgttc
     6841 gccaacgatg ccggcgacgc ttcgctcgtc gggtggagca ccatcggccg gcaagtggag
     6901 gccgccggca acatgctgcg cagtgcagag cccgagctcg tcgcgcacgt tcgcacgaag
     6961 atcgagacgt actacgcgga gctcgagcgg ctcggtcgca cggacgcgca gacgtcgggt
     7021 gccgcagctg caacgccgaa atcgccaaca cgttggctgc gtcgcatcgc gctcgcaccg
     7081 ctcgccgtgc ccggcttcgt gctctacggc ctgccctatt tcatcccgcg gctcgtcgct
     7141 cggtcgagcg atgccgacgc tgtatcgacg gtgaagctcg ggactgcgct cgtcgtctat
     7201 cccgtgtggg ccgcgacgct cgtcggactc tcgctcgcct tcatcccgcc gccgctgtca
     7261 ttgttcgccg ccgcgatcgt gatcgcctcc ccgtttgcgg cgcttcactg gctcgacgcg
     7321 tactggaacc gcacgcctga ccgcgagctg acacccgacg agctcgcaac cttggctcga
     7381 cttcgcatcg ccgcgcgcac tgcgatcggc gaagcgcgca cccggctcgg ctaggctgca
     7441 accgcgccga tgcggcggtg tcccccaggt gcatgcacgc actcatcgtc gttgccgcac
     7501 tcgccaccga ttggttcgat gggctcaccg cgaaagtgat cgaggacgta aaggccggca
     7561 agccgctggt cgtcgaggtt cacgtcccgc tgtgcgacaa cgaattcact cggtgcggca
     7621 acgcgaagct cggcgacggc gataaccccg agaccaatct ctattggtcg acgacacccg
     7681 gcctcggcgt gtggttcgca cgaaaggccg gcggctggca gcgcgtcttt cacgcgaccg
     7741 acggcaagga caccggcgac aaggacgtgc tcgagctaca cgtctatcgc cggacgatca
     7801 cgacgtccgc ggcgtggaga aagctcggcg cgccgaagag ctacgaagtc gacctcgtcg
     7861 tccatggctg gcgcggcagc gcgatcgatc gagcactcgc tgcctacgcg tccgacgtct
     7921 ccggcgaggg cacgcgcacg atcacgctcg cgaacaagac ggagatcgcg gcgggcggcg
     7981 ccgcgcgcat cgtcgcgttc gtcggccaca accggctcat ggacctcgac aagttcgagt
     8041 ggccgaaacc cggcgccgac atcaaaggca cgatcgcgat cgcctgccac acgaagagct
     8101 acatgaaaga agacgtcccc gcggcatcac gcgtgccgct gctcatgacg aacgacttct
     8161 tgttctcgaa tgcagcacca ctcgaggcag cggtgctcgc gttcgcacgc ggcggcggct
     8221 acgccgcgat tcgcaaagat gcggcgaccg cttacgctgg tgtccagaag cgcgacgtga
     8281 agcgcgtcat cggcgcattc acgaatcctg ccgacaagaa gtggtgatca ctgcgcgcac
     8341 gcggcagcgt cggcgcaaat ctcggcgtcc gttcgcttgg tgcggtacac gcggagcacg
     8401 tcgatcttgc cgatgaatcg atcgagtccg tcggggctgt tgccgccgac ttcaccgcca
     8461 tcgctgggag aggtctcgag cggcgtcgga aacgtggcga cggccgcgac ctgtccgttg
     8521 atgtacatga tcgaaacgct attggcggac gtgcacgcga cgtgcgtcca ttgattgata
     8581 gcaatcccac cggcctcgaa cgtgctccct gcggtgcaca ccagcacggc gccggcgcta
     8641 gcgggtttga gaaacatgcc gtattgaccg ttcacgtcga tcacacccat gcgccctgcc
     8701 ggcgacggtg tttgcgtcgg atagatccac gcttcgatcg agaccgcggc aacgtcgaga
     8761 aggacgctgt cctgtatcgc gatgcgagat gccgtgtcga gcagtgctgc ttttccgcgc
     8821 tgtcctgacg agtacgcgac gttcacaccg gcaacggaat tcgggctcgg tgcgaggtcc
     8881 ttgacggtgt cctcgaagtc gaagcacgcg acgaggttca tgtcgtttcc gcacgctgca
     8941 aacggcgccc catccggcat cgcggtatcg cgatccacgc gcggagcgtc gtcgaccacc
     9001 tgctgcgccg gcggttcgag cccgaggacc gagcggcatc ccgtcaccag cacgagagcc
     9061 accgcagacc gcatggctca gcgtacccga tcagtcgtcg cgcgggccaa gaagcggaag
     9121 catgggcggc gcatcgtcgg tatcgtttgc ggtgacgcct cggcgcgaca ggattttgag
     9181 cgagcgcaga atcttgtccg cagcgggcca ctccggtgac gccgatcgaa ccgtgacgat
     9241 caagccgcca tcgagcgcga gatcattcgg cgcgaccgca aacacgtaga cgtacggcaa
     9301 cgcgggtcgg gcatgtccca tagggcgcac gacctcgatg tcggcgcgat agccgctcgc
     9361 accaggcaac gcgaccggga tcggcggcag cacgcgcgag ccgggctcgc tgttgctatc
     9421 gactgcggct tccatcttct cttcgaggat gccgtcgcga tcgatcacga gcgcagcgtg
     9481 gaagacgctg acctcgagct caccgatcgt ctcgccgctc gcgcgacgct cgcgacacgt
     9541 cagcacgtcg ccagcctgca cctcgacctc cgtcggcgtc tggatctcga agccgatgcg
     9601 ccgggaggcg gtaggaagct ggcgatagct catggcctac tccctcgata ctattgcggg
     9661 agcggccggg agtgggatct ttcagtcgtc gacggaagtg cgataattct ttgcccattt
     9721 gggccgaaac atcagggcga tgcgcggtcc ggcgctcgcg accttcggga ttccgtggcg
     9781 ccacgttcgc tggcaggtcc cgcccatcac gacgagatcg ccgcgtccga gtggcagcgc
     9841 gatcgacggg ccgccctccg ccggtcgcag catgaagcga cgcgacgcac cgagtgacac
     9901 ggtcgcgacg agcgcttcga ccatgttgcg cgccgtcgtg tcgccatgaa acgcaacgct
     9961 gtcgttgcca tcgcgataga gcgcggccgt ggtgaagaca aaatcctcgc cgtacttcgc
    10021 ggagatcgcg gcgcgcatct cgtcgacgat tccttcgcgt ggcacgcgcg cgaggagtcg
    10081 cggaacctcg atcgtcttgt cgtacatctt catcgtctcg cgccgccact gcaggctgcc
    10141 ttcgagctcg tcgaacagcg catcgtgccc ggacacccag ctcggcacgt gatcgaccca
    10201 cgccccgtga gaaagctctc tacgctcgag cgtccggaat tcgcgatcga atgacggtgc
    10261 gccgcgcccg agtaagtcga gctgcctctc cacgagctag gcttagcgca tgggcgaagc
    10321 gaagggcggc cgggaccgaa agaccacgac gaagatgagg aagatcgccg cgcccgccgg
    10381 ttactcgggt acgcccctgc cccaaaagct aggcatcaaa gaggaccacg aggtcgccgt
    10441 gctcgatgcg cctcggggat tcttcgacac gctcggcaag cttccgagtg gcgcgaccgt
    10501 gcagacgcgg ctcgccggca agatgccgct cgacgtcgtc gtcgcattcg cgacgaaggt
    10561 cgccgacctc acccgcgcga tcaaaaacgt acgcccgcgg atgcagccgg ccgccggcct
    10621 gtggatcgcg tggccgaaga agtcgtccgg cgtgccgacc gaaatcaccg aggacgtgat
    10681 ccgcgcgatc gccctgccaa ccggcctcgt cgacaacaaa gtctgcgcga tcgacgagac
    10741 ctggtccggc ctgcgcctcg tcatccgcaa agagaatcgc tagcagccct aaacacggca
    10801 tcgagcattg gctcggtgag gcggccggtg aacgtgttct gctggctgac gtggtagctg
    10861 ccgaggagcg agtacgggcc gagcgtcgag cgggcgccgt gtccgaacgt cggacggggc
    10921 gtgggaagcg ggtggccgag ctcgcgcgcg gcgcgaagga cgccgtccca cgcgaaggcg
    10981 ccaagagcga taatcgcttt cacttttggc agcgcctcga gctcgcggac caagaatggc
    11041 aggcacttgt cgcgctcgat cggcgttggc ttgttgccgg gcggcgcgca ccgcacgacg
    11101 accgagacgt acgcgtcggt gaggcgaagg ccgtcgtcga gctctgtcga gtgcggctgg
    11161 ttcgcgaagc ccgcgcggtg gagcgcgccg tagagccagt cgcctgactt gtcgccggtg
    11221 aatacgcggc ccgtgcgatt gccaccgtgt gccgccggcg cgagaccgac gacgagcagg
    11281 cgcgcctttg gatcgccgaa gcccgtgatc gcgcggcccc agtaggtgtg accggcgaac
    11341 cgcttcggtg cggccgcgcc cgcggcttcg
//
LOCUS       DATLBZ010000202         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6061740, whole genome shotgun sequence.
ACCESSION   DATLBZ010000202 DATLBZ010000000
VERSION     DATLBZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6061740"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1967)
                     /locus_tag="VMZ53_10560"
     CDS             complement(<1..1967)
                     /locus_tag="VMZ53_10560"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HUS28947.1"
                     /translation="MRVALLLCVLLAFAPRNASAQSLTTGAIQGVVKDAESGEPLPGV
                     EVWIGGQFTTTDGDGAYKITEVLPGTYDIVFKFEAASVTKSGVQVGANDVRNINQRMR
                     IGESIHIDGSKDDIRIRTDSTAKEKRIDRKQIEKLPNPGQSAEDTIGSVPGTQNDGTG
                     VAVSGSSGLENRWVVDGVDMTGLTYGNVGTSVLNDFVEEIIAVAGGYNAEYGRATGGI
                     INIITRRGTDEIRGSVFGVVKPGWLTARAHVAPSNSSSIDVSGDNVYDGHVGFEVGGP
                     IVKKRAWFYLGAAPQLARTDYTRTIKRQTDCRVTGQDGKLSTCDPHLADGDPDLDPST
                     GFYVTDKVDEEVRSATSRSTQIIGKLNVAASPSEQGQLSLIAQPSSSRSPALLGHPST
                     ATKSSGLTTDTAARWSSKFNDSADEVEATLAWHRSTFNSGSVDPALDSVPLQILQGGS
                     LGGISGYGGETAATIAGCGDGGPDDAYSLITNCPMNTTYAIGGPGSISRDVEQRRSGR
                     IGWTHRLKAFGTHEIKAGIDAENNSKVTTRVYSGGALIQNAGSVIQLNRYAEIAPPGS
                     TDPRYDHMCSTPDIGGGTTGTGSTKRFQCRYLGGLDDPSVYVGGQTINWGAYLQDSWQ
                     IRRSLVFNAGLRYEEQRLRYAERLRNQLDSLT"
     gene            2053..>2267
                     /locus_tag="VMZ53_10565"
     CDS             2053..>2267
                     /locus_tag="VMZ53_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28948.1"
                     /translation="MSQRLAQLTADITACRRCKRLVRCREAAGAAAPKRFAGHTYWGR
                     AITGFGDPKARLLVVGLAPAAHGGNRTG"
BASE COUNT          337 a          750 c          743 g          437 t
ORIGIN      
        1 gtcagcgagt cgagctgatt gcgaagacgc tcggcgtaac ggagccgttg ctcttcgtag
       61 cgaaggcccg cgttgaacac gagcgagcgg cggatctgcc atgagtcttg gagatacgcg
      121 ccccagttga tcgtctgccc gccgacgtac accgacggat cgtcgaggcc gccgaggtag
      181 cgacattgga aacgcttcgt gctgccggtg cctgtcgtgc cgccgccgat atctggcgtc
      241 gagcacatgt gatcgtagcg agggtccgtg ctgccgggcg gcgcgatttc ggcgtagcgg
      301 ttgagctgga tgacgctgcc ggcgttttgg atcagcgcgc cgcccgagta gacgcgcgtc
      361 gtcactttcg agttgttttc ggcgtcgatg cccgccttga tctcgtgcgt gccgaacgct
      421 ttcaggcgat gcgtccagcc gatgcggccc gagcggcgct gctcgacgtc acgcgagatc
      481 gagcctggcc caccgatcgc gtacgtcgtg ttcatggggc agttcgtgat cagcgagtac
      541 gcgtcgtcgg gcccgccgtc gccgcagccg gcgatcgtcg ctgcggtctc gccgccgtaa
      601 ccgctgatgc cgccgaggct gccgccttgc aggatctgca gcggcacgga gtcgagcgcc
      661 ggatcgacgc ttcccgagtt gaacgtgctg cgatgccacg cgagcgttgc ttcgacctcg
      721 tcggcgctgt cgttgaattt cgaggaccag cgcgccgctg tatccgtcgt gagccccgag
      781 ctcttcgtcg ccgtcgacgg atggccgagc agcgcggggc tccggctcga cgacggttgc
      841 gcgatgaggc tcagctggcc ttgctcgctc gggctggccg cgacgttgag cttgccgatg
      901 atctgcgtcg agcgggacgt cgcgctgcga acctcttcgt cgactttgtc ggtgacgtag
      961 aagccggtcg acggatcgag atccggatcg ccgtcggcga ggtgtgggtc gcacgtcgag
     1021 agcttgccgt cttgtccggt gactcggcag tcggtctgcc tcttgatcgt gcgcgtgtag
     1081 tcggtgcgtg cgagctgcgg tgcggctccg aggtagaacc acgcacgctt cttgacgatc
     1141 ggcccgccga cttcgaagcc gacgtggccg tcatagacgt tgtcgccgct gacatcgatc
     1201 gacgacgagt tgctaggcgc cacgtgcgca cgcgcagtca gccaccctgg cttgacgacg
     1261 ccgaacaccg agccgcggat ctcgtcggtg ccacgtcgcg tgatgatgtt gatgatgccg
     1321 ccggtcgcgc gcccgtactc cgcgttgtag ccgccggcga ctgcgatgat ctcctcgacg
     1381 aagtcattga ggacgctcgt gccgacgttg ccgtatgtca ggcccgtcat gtcgacgcca
     1441 tcgacgaccc agcgattttc gagaccgctc gagccgctga ccgcaacgcc cgttccatcg
     1501 ttctgcgttc ccggcacgct gccgatcgtg tcctctgccg actggccggg gttcgggagc
     1561 ttctcgatct gcttgcgatc gatgcgcttc tccttcgccg tcgagtccgt acgaatgcga
     1621 atgtcgtcct tgctgccgtc gatatggatc gactcgccga tccgcatgcg ctggttgata
     1681 ttgcggacgt cgttcgcgcc gacctgcacg ccgctcttcg tcacgctcgc cgcttcgaac
     1741 ttgaagacga tgtcgtaggt gccgggcagc acctccgtga tcttgtaagc accgtctccg
     1801 tcggtggtcg tgaactgacc gccgatccac acctcgacgc cgggcagcgg ctcacccgac
     1861 tccgcatctt tcacgacacc ctgaatcgca cccgtcgtca gcgactgcgc cgaggcattc
     1921 cgtggcgcga acgcgagcag cacgcacagc agcaaagcaa ctctcatcct tctgtcacta
     1981 cgtggtcacc ggcgagcgcg acaccgtgcg gcgatcaaga cctgtcacac gctgcgtgcg
     2041 acactcgcaa aaatgtcgca gcgactcgcg cagctaactg ccgacatcac ggcgtgccgc
     2101 aggtgcaagc gactcgtgcg ctgtcgcgaa gccgcgggcg cggccgcacc gaagcggttc
     2161 gccggtcaca cctactgggg ccgcgcgatc acgggcttcg gcgatccaaa ggcgcgcctg
     2221 ctcgtcgtcg gtctcgcgcc ggcggcacac ggtggcaatc gcacggg
//
LOCUS       DATLBZ010000203        10946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_946495, whole genome shotgun sequence.
ACCESSION   DATLBZ010000203 DATLBZ010000000
VERSION     DATLBZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10946
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946495"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1326)
                     /locus_tag="VMZ53_10570"
     CDS             complement(88..1326)
                     /locus_tag="VMZ53_10570"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28949.1"
                     /translation="MIGEIVDGHEILRPLGAGGMGEVYLARAASGTLRAFKVVRTDRQ
                     TGPQAAARFRREVLALGKLQHPGIIRILDAGTLPNGALYLGMEYVGGPDLQAAVGWDG
                     PFPVADALRIIVQLAAALAYAHGAGVIHRDLKPANVILEEGDAGRAKIIDFGLAKIVE
                     NEGLTRLTEDKQALGSPLYWAPEQSSNTDVGPPADVYSLGGILYFLMSGEPLFKPRPA
                     VAMVYAHTHEQPQPLAMRCRGIELPAGLDELAARCVAKLPAHRPTAAELVIELDRMLA
                     TLPSTIGQRRAAQRLFTSTGLSNMQDALTNQMRQVLLDLASVLQRPTDDIDRIQQELS
                     ELELDFAVLESDVQMAVDPEVEERHAGISQQMAQLQAALTDAYRELFDAVSADRNRAV
                     PDAHALFSELDSLVEQYRSL"
     gene            complement(1348..2640)
                     /locus_tag="VMZ53_10575"
     CDS             complement(1348..2640)
                     /locus_tag="VMZ53_10575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28950.1"
                     /translation="MIGTILHDRWRILAELGKGGMGEVWLAEHLELNRKEAIKILMPG
                     LAGDPQFVSRFRREARAVNRLRHPNIIAIYDFGQLPDGRFYLSMEYADGQSVYNLVKR
                     DEHFVVPRALHVLGQLAYAVHHAHSRGVVHRDLKPDNLILVGEDEMLKVLDFGVAKIV
                     ASDHNESVALSSNNLVWGSPRYMAPERVRGVGDDPRSDIYAIGCIAFELVIGSAPFTG
                     NSDDVIRAHLKQTPEAPSTWRPSLGIPTELDAIILRCLAKKPEDRFQTAADLYAAITK
                     VPGFPTAKAEPRRRFVPVPRRPTDHGAESSGPTDARGALRMVADAMLAAGATDPRLRA
                     GTIALRDCEQSLTGLEAAQNALEHELAALGKTGDRGLEENLAGIVQSRGAALDKAMAA
                     YDVLEQVVEELLPAYATDETVAPLAAKLAVVKRQRQSQ"
     gene            complement(2766..3710)
                     /locus_tag="VMZ53_10580"
     CDS             complement(2766..3710)
                     /locus_tag="VMZ53_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28951.1"
                     /translation="MVLRVVVLLAMLGSVAIADGDPDGAKPWTAGKSKAEQDAAFHLY
                     DEANELFAKGEYVKALEKYDAALAAWDHPGIRYNRAVCLINLDRTVEAYQDLHLALKY
                     GEEPIGKDLYNEGLNYQKLLAKQVAEIEIVASDKGAKISVDGKPLALTAGRATLTLAT
                     NAPHEITAAKPGYQTFRKPLDRLPPGQTTRIDIRLELLERGRWKPWAVIAGGAVISGV
                     GVYAYSLARSDLDEYEKAIANFCPCTSEADLEARGKIAHDLPGSAHTKEIIAYSALAI
                     GGATIATGIVLVIRGRGETHVAPAVSRDSAGVSLLGRW"
     gene            complement(3728..4138)
                     /locus_tag="VMZ53_10585"
     CDS             complement(3728..4138)
                     /locus_tag="VMZ53_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HUS28952.1"
                     /translation="MVQAAARQSAAGEITTLLGRGATFEGKLTFEGTVRIDGRFKGEV
                     FTDDVLVIGEGAIVEATIDVGEVIIQGTVIGNITAKRSIEIHAPGRVKGDLHTPSLQI
                     DKGVMFEGRSFMEAIVANRPAAQQPQAAKPQPVK"
     gene            complement(4165..5610)
                     /locus_tag="VMZ53_10590"
     CDS             complement(4165..5610)
                     /locus_tag="VMZ53_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28953.1"
                     /translation="MDLSRRLALLLVLAACGSHAHGNTDAGVDGGYVADACEGLRCFQ
                     AGDLCAQKGLPPTTLSGTVYAPNGTLPLYGVDVYVPETDPGPLMAGAICARCDQGLQG
                     GAIVSTKTDEYGRFKLENVPATASVPLVIQTGKWRRQLTIANVAACEDQPLAATETRL
                     PKNHMEGDLPKIAISTGGADAFECLPLKLGIDPIEFTNPTGTGHIHMYTNPVGGPDAG
                     GSPAGQGANKFATTNWTGGTGNFGASQTLWANVDNMKPYDIVILSCEGDQYPGSKGTT
                     GLAALKSYADLGGRVFLSHWQNVWLEGATGATAPPEWGVGAGIGGPVATWNNSGTSFT
                     SPPYAGGTSLPDIIDEVANPKGASFATWMMGPEVMGSTIRGQIPIKDGKQTCTGVGMG
                     TEQWTTWTNQVVPPATFPQNFQFTTPRELSSDQRCGKVVFSDMHVSSTASSDPLTPFP
                     GGCNLATPLSQQEKALAFMFFDIASCVGSIF"
     gene            complement(5626..8406)
                     /locus_tag="VMZ53_10595"
     CDS             complement(5626..8406)
                     /locus_tag="VMZ53_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS28954.1"
                     /translation="MAERQFGPYRLVRQIAVGGMAEIHLAKTKGIAGFEKYVALKMIH
                     PNFAEDEQFIQMLVDEAKIAVQLNHGNIAQTFDLGRVGDQFYITMEYVDGADLYKILR
                     RGSEIDFEMPLDVCAFVAKEIASALDHAHRKRDHNGKTLGIVHRDVSPQNVLISFSGE
                     VKLVDFGIAKATMKARQTAVGVIKGKYYYMSPEQAWGDQIDYRSDIFSAGIVLYEMMT
                     GQMLYLEEDLHKLLDMVRKADIAPPSTLRKGIPPQLEKIVMHALDKDRRNRYQSASDF
                     ASDLERFLHTYSPVFTASKVSTVIKQVIGAPTEVPAEDYEVEVRDGPMSTHTLSTDDL
                     ILDKEELHDENSVIFRVDDLKPKHDAAKKPVPAGPRVTRQAEIKDAAPPKPAAIPSVP
                     RKAPTPAAGQQRAPTAKPRDKNESTRQVEPAPLPPDYPGEDSGLLSFKTPPPLAAPAW
                     SKRHDSESTEPGDVGDLENIGESTMITGAPKGFGGFMMDNASEDGVDATVVSSGPPSA
                     EQHDTAEEALDDDEDAPTLGGGANDEDGPTIQRDYSKPKDNRQPKVAAKRGPPPAALA
                     ANIQAPAVSALHKPRASRKTPAGGVPAVQPNVLQAIVNQQGGAPMPTPPRQNPQMPTQ
                     ALQPPMQQQMPPPQQPQMQQPMPPQAYNGYAADASGLPMTPPQGVPVAQQQYGQQQPY
                     GYPQYPPQQPYQQPSYPGQAYQQPVSPGALYQLQPYGAPPPKPMSLTGQLRLFEADEL
                     PAHYNVSSGGRWIKLAIAGILAISVAAGVTFFIIKSTRDSAPTVGRVNIDSVPPGAEV
                     VFDGTRLAGATPMTVDSVPVGTRHEIKVELPRHKPYVETVDIPKTGGEVPVKAMMVPI
                     TGKLRVITTPDGAEIWIDGKLRGRAPTTIADIDMGSAKLLELRLKDYKPYQQSLQWPA
                     NGEINIDAKLQK"
     gene            complement(8399..9409)
                     /locus_tag="VMZ53_10600"
     CDS             complement(8399..9409)
                     /locus_tag="VMZ53_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28955.1"
                     /translation="MSKRRVLAISSDLDQLRRIVATLERAGAEVDAVRSPSAIAAEVI
                     PHRYIFYATSDAHAERSSERSIEALQTLVPKLRDRAHVAIITPKASLGDLTKYLRDDR
                     INHVIAGDEDLENGVSVTAGKLLTGDIFGIEKYLPENTPVHYARLRDFEGRGKAIQTV
                     LDFAEESKIRRQVRSAIGTVCEELLMNALYDAPVDADGKQIFAEVDPHDRTKTRSPRP
                     VSIRYAATEDMFAVAVRDRFGRLAKNTILAYIEKCLTSGNQIDRKTYGAGLGLYLVAN
                     AAATYVVNVAYGIATEVVCTFDRGAKAPLRLVGVFVHPGGAEMLKQGPTPDSIDQDIP
                     RG"
     gene            complement(9468..>10946)
                     /locus_tag="VMZ53_10605"
     CDS             complement(9468..>10946)
                     /locus_tag="VMZ53_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS28956.1"
                     /translation="VRYGSSDPRWDGITFPTGTANLNDPTGTARENRWDTFDRYVVGE
                     YRDRLASGKAGLTLRAYYQQFVRNFEPINVLAPSQLIQGGLAFQTNLSSDRVGGAFDG
                     DVELSKKMRVLYGAEAFREWKPVDTTSSIQGSGNQADFIAPSDLTRLPIPCPRTFDPT
                     TNVIVPVAGCPQTFAYDATRTVVGAYADPQFRPTKGLILDAGARVQVAPDSLGTVSYP
                     VNYTVGGAIVWNFIPNWHLKLNYTQGFRPPVFNNTSANGEAVVIAGSPDLKVETSDAT
                     QVEVNARIFKGDRRIRELSFRIDGSYTRVENLIQVNTGSYGNSGERGIASGEFLGKLY
                     IQGGHRVELGYTWLRVATADRGFIRSLPEHWFNLSTVFNMIPGKLTGTSNLRVTGAAE
                     DPNRLVEYRGATYDMNGNPMGAVTVNPTDLVMDRIQPIADLSVGISWTPIEKLLVRAT
                     VYNALMSHTYQPDVFFDYEPHLEYMPNPYEGLRAYLTALYQY"
BASE COUNT         1727 a         3495 c         3607 g         2117 t
ORIGIN      
        1 ctttgacctt gtcgacactc gtcttcggct cttcgtcgaa gccatgcgac ggcttgtccg
       61 catcgatgat ggtgcgatcg agcggcttca tagtgagcgg tactgctcca cgagcgagtc
      121 gagctcgctg aacagggcgt gcgcgtcggg caccgcgcga ttgcggtcgg cgctgaccgc
      181 gtcgaacagc tcgcgatagg cgtcggtgag cgcggcttgc agctgcgcca tctgctgcga
      241 gatgccggcg tggcgctctt cgacttccgg atcgacggcc atctgcacgt cggactcgag
      301 gaccgcgaag tcgagctcga gctcggagag ctcttgctgg atgcgatcga tgtcgtcggt
      361 cggacgctgc aggacggacg cgagatcgag cagcacttgg cgcatctggt tcgtcagcgc
      421 gtcctgcatg ttggagaggc ccgtgctcgt gaacaggcgc tgtgcggcgc ggcgttggcc
      481 gatcgtcgac ggcaatgtcg cgagcatgcg gtcgagctcg atcacgagct ccgccgcggt
      541 cggccggtgc gcgggaagct tggcgacaca tcgcgcggcg agctcgtcga gaccggcagg
      601 cagctcgatg ccgcgacaac gcattgcaag cggctgcggc tgctcgtgcg tgtgcgcgta
      661 gaccatcgcg accgccgggc gtggcttgaa gagaggctca ccactcatca agaaatacaa
      721 aatgccgccg agcgagtaga cgtcggccgg cggaccgacg tcggtgttgc tgctctgctc
      781 gggagcccag tagagcggcg agccgagcgc ttgcttgtcc tcggtgagcc gcgtgagacc
      841 ttcgttctcg acgatctttg caagaccgaa gtcgatgatc ttcgcgcgac ccgcgtcgcc
      901 ttcttcgagg atcacgttcg ccggtttcaa gtcgcgatga atgacgcccg cgccgtgcgc
      961 gtatgcgagt gctgcagcga gctgcacgat aatgcgcagc gcatcggcga ccgggaaggg
     1021 gccgtcccat ccgactgcgg cttggaggtc cggaccgccg acgtactcca tgccgaggta
     1081 gagcgcgccg ttcggcagcg tccctgcatc gaggatgcgg atgatgccgg gatgctgcag
     1141 cttgccgagc gcgagcactt cacgcctgaa ccgagcggcg gcttgcggtc ccgtctgcct
     1201 gtcggtccgg acgaccttga atgcgcgcag cgttccggac gccgcgcgtg ccaggtagac
     1261 ctcgcccatg ccaccggcgc cgagtgggcg cagaatctcg tggccgtcga cgatctcccc
     1321 gatcacgtac caatagggta acacccccta ttgggattgg cgctgtcgct tcacgacggc
     1381 cagcttcgcc gccagagggg ccaccgtctc gtcggttgca taagcgggca acagctcctc
     1441 gacgacttgc tcgaggacgt cataggccgc catcgccttg tcgagcgcag cacctcgcga
     1501 ctggacgatg cctgcgagat tctcctcgag cccgcggtcg ccggtcttcc cgagcgcagc
     1561 gagctcgtgc tcgagcgcgt tttgcgccgc ttcgagtccc gtcaatgact gttcgcaatc
     1621 gcgaagcgcg atcgtccctg cacgtaaccg agggtcggtg gctccggcag cgagcatcgc
     1681 gtcggcaacc atccgcagcg ctccgcgcgc atcggtgggc ccgctcgatt cggccccgtg
     1741 atctgtcggc cgccgcggca ccggcacgaa gcgacggcgc ggctccgcct tcgcggtcgg
     1801 gaatccgggc accttcgtga tcgccgcgta gagatccgcc gccgtttgaa atcggtcctc
     1861 gggtttcttc gcgaggcagc gcaggataat cgcgtcgagc tccgtcggaa tcccgagtga
     1921 cggtcgccat gtgctcggcg cttcaggcgt ctgcttgagg tgcgcgcgga tcacgtcgtc
     1981 ggaattcccg gtgaacggcg cgctgccgat cacgagctcg aacgcgatgc agccgatcgc
     2041 gtagatgtcg ctgcgtggat cgtcgccgac gccgcgtacg cgctccggcg ccatgtagcg
     2101 aggcgagccc cacacgagat tgttcgaaga cagcgcgacg ctctcgttgt gatcgctcgc
     2161 gacgatcttc gcgacgccga agtcgagcac cttcagcatc tcgtcctcac cgacgaggat
     2221 cagattgtcg ggcttgagat cgcgatgcac gacgccgcgc gagtgcgcgt gatgcacggc
     2281 gtacgcgagc tggccgagca cgtgaagcgc gcgcggcacg acaaagtgct cgtcacgttt
     2341 gacgaggttg tagacgctct gcccgtccgc gtactccatc gagagataga agcgaccgtc
     2401 gggcaactgg ccgaagtcat agatcgcgat gatgttcgga tgccggaggc gattgacggc
     2461 acgtgcttct cgccgaaacc gcgagacgaa ctgcggatcg cccgcgaggc cgggcatcag
     2521 gatcttgatt gcctctttgc gattcagctc gagatgctcg gcgagccaca cctcgcccat
     2581 gccgcccttg ccgagctcgg ccaggattcg ccagcgatca tgcagaatcg tgccgatcac
     2641 ctgattccca tcttagatga tttccgtgtg agcgtccgtc gggcgggtca ccggctggca
     2701 tcgtgcgcac gatggatccc atccagccgc ctgccctccc cggcggcgcg cagatccgac
     2761 agtagctacc agcgcccgag caagctgacg ccggcggagt cgcggctcac ggcaggcgcg
     2821 acatgggtct cgccgcggcc gcgaatcacg agcacgatgc ccgtcgcgat cgtcgcaccg
     2881 ccaatcgcaa gcgcgctgta cgcgatgatc tccttcgtgt gcgcgctgcc cggcaagtcg
     2941 tgcgcgatct tgccgcgggc ttcgagatcc gcttcgctcg tgcatgggca gaagtttgcg
     3001 atcgctttct cgtactcgtc gaggtcgctc cgcgcgaggc tgtacgcgta gacgccaacg
     3061 ccgctgatga cggcgccgcc ggcgatcacc gcccacggct tccagcggcc acgctcgagc
     3121 agctcgaggc gaatgtcgat gcgcgtggtt tgtccgggtg gcagacgatc gagtggcttg
     3181 cgaaatgtct gatagcccgg cttcgccgcg gtgatctcgt gcggcgcgtt cgtcgcgagc
     3241 gtcagcgtcg cgcggccagc ggtcagcgcg agcggtttgc catcgacgct gatcttcgcg
     3301 cctttgtcgc tcgccacgat ctcgatctcg gcgacctgct tcgcaagcag cttttgatag
     3361 ttgaggccct cgttatagag atccttgccg atcggctctt caccgtactt cagcgcgagg
     3421 tgcagatctt gataggcctc gacggtgcga tcgagattga tgagacagac ggcgcggttg
     3481 tagcgaatgc cggggtggtc ccacgccgcg agcgccgcgt cgtatttctc gagcgccttg
     3541 acgtactcgc ccttcgcgaa gagctcgttc gcctcgtcgt agagatggaa cgcggcgtcc
     3601 tgctcggctt ttgacttgcc ggcggtccac ggcttcgcgc cgtccggatc tccgtccgcg
     3661 atcgcgacgc tacctagcat cgcgagcaga actacgacgc ggaggaccat gcctccacgt
     3721 tacaccatca cttgacgggc tgcggcttcg cggcttgcgg ctgctgagcg gccggacgat
     3781 tcgcgacgat cgcttccatg aacgagcgcc cctcgaacat gacgcccttg tcgatctgca
     3841 ggctcggcgt gtggaggtcc cccttcacgc gccccggagc gtggatctcg atcgaacgct
     3901 tcgcggtgat gttgccgatc accgtgccct gaatgatgac ctcgccgaca tcgatcgtcg
     3961 cttcgacaat cgcgccttcg ccgatcacga gcacatcgtc ggtgaacacc tcgcccttga
     4021 acctgccatc gatgcggacc gtgccctcga acgtgagctt gccttcgaag gtcgctccac
     4081 gtccgagcag agtcgtgatc tcgccggccg ccgactgtct cgccgctgct tgcaccatag
     4141 gttcggcagc ctatagcaac gccgttagaa gatcgagccg acgcaggaag cgatgtcgaa
     4201 gaacatgaac gccaacgcct tctcctgctg cgacagcggc gtggcgagat tgcagccgcc
     4261 cgggaacggc gtcagaggat ccgacgacgc ggtgctggac acgtgcatgt cgctgaacac
     4321 gaccttgccg cagcgttgat ccgacgacag ctcgcgcggc gtcgtaaact ggaagttctg
     4381 cgggaacgtc gcgggcggga cgacttggtt cgtccacgtc gtccactgct cggtccccat
     4441 gccgacgccg gtgcacgtct gcttgccgtc cttgatcgga atctgaccgc gaatcgtcga
     4501 gcccatgact tcgggtccca tcatccacgt cgcgaacgat gcgcccttcg ggttggcgac
     4561 ctcgtcgatg atgtcgggga gcgacgtgcc gcccgcgtac ggcgggctcg taaacgaggt
     4621 gccgctgttg ttccacgtcg cgacaggtcc gccgatgccc gcgccgacgc cccactccgg
     4681 cggtgcggtc gcgccggtcg cgccctcgag ccacacgttc tgccagtgcg agaggaacac
     4741 gcgaccgccg aggtcggcgt acgacttcag cgccgcgagg cccgtcgttc ccttcgagcc
     4801 gggatactga tcgccttcgc acgacaggat gacgatgtcg tacggcttca tgttgtcgac
     4861 gttcgcccac agcgtctgcg atgcgccgaa gttgccggtg ccgcctgtcc agttcgtcgt
     4921 cgcgaacttg ttcgcgcctt gacctgccgg gctgccgccc gcatcgggac caccgacggg
     4981 attcgtgtac atgtgaatgt ggcccgtgcc cgtcggattc gtgaactcga tcggatcgat
     5041 gccgagcttc agcggcaagc actcgaacgc gtcggcgccg cctgtcgaga tcgcgatctt
     5101 cggaagatca ccttccatgt gattcttcgg caggcgcgtc tcggtcgctg cgagcggttg
     5161 gtcttcgcac gcggcgacgt ttgcgatcgt cagctgacga cgccacttgc cagtctggat
     5221 cacgagcggc acgctcgcgg ttgcaggaac gttctcgagc ttgaagcgac cgtactcgtc
     5281 ggtcttcgtc gagacgatcg ctccgccttg cagaccttga tcgcagcgcg cgcagatcgc
     5341 gcccgccatc agcggtcccg gatcggtttc gggaacgtag acgtcgacac catagagagg
     5401 cagcgtgccg ttcggcgcgt acacggttcc ggacagcgtc gtcggcggca gacctttttg
     5461 cgcgcagaga tcgccggctt gaaagcagcg aagtccttcg catgcgtctg cgacgtaacc
     5521 accatcaaca cccgcgtccg tgttgccgtg agcgtgtgaa ccgcaggctg cgagcacgag
     5581 cagcagtgcg agccggcgag agaggtccat attcatattc tagagctact tctggagctt
     5641 ggcgtcgata ttgatctctc cgtttgccgg ccactgaagg ctttgctgat agggcttgta
     5701 gtccttcagc cggagctcga gcagcttcgc cgagcccatg tcgatgtcgg caattgtcgt
     5761 cggtgcacgt ccacgtagct tgccgtcgat ccaaatctcg gccccgtcgg gcgtcgtgat
     5821 gacgcgcagc ttcccggtga tcgggaccat catcgctttg acgggtactt ccccgccggt
     5881 cttcgggata tcgacggtct cgacgtacgg cttgtggcgt ggcagctcga ccttgatctc
     5941 gtgacgtgtg cccacgggca cactatcgac cgtcatcggg gtcgcacccg ccagccgggt
     6001 cccgtcgaag accacctccg cgccgggcgg cactgagtcg atgttcactc tgccgacggt
     6061 cggcgcgctg tcgcgggtgg acttgatgat gaagaaggtc acacctgcgg cgaccgagat
     6121 cgcgaggatg ccggcgatcg ccagcttgat ccagcgcccg ccgctcgaga cgttgtagtg
     6181 cgcgggcagc tcgtccgctt cgaacagccg gagctggccg gtgagcgaca tcggcttcgg
     6241 cggcggcgcg ccgtagggtt gcagctgata gagcgcgccg ggactgacgg gttgctgata
     6301 cgcctgtccc ggatacgacg gctgttgata cggctgctgc ggcggatatt gcggatagcc
     6361 gtacggctgt tgttggccgt attgctgctg cgcgacgggc actccctgcg gcggcgtcat
     6421 cggtagtccc gacgcgtcgg cggcgtagcc gttatatgcc tgcggcggca tcggctgctg
     6481 catctgcggc tgttgcggcg gcggcatctg ctgctgcatc ggcggctgca gcgcctgcgt
     6541 cggcatctgc gggttttgcc gcggcggcgt cggcatcggc gcaccgccct gctgattgac
     6601 gatcgcctgc aagacatttg gctgaacggc aggcacacca ccggcgggtg tcttgcgaga
     6661 tgcgcgcggt ttgtgcagcg cgctgaccgc aggcgcttgg atgtttgcgg caagcgcagc
     6721 gggcggcgga ccgcgctttg ctgccacctt cggctggcga ttgtctttcg gtttgctgta
     6781 atcgcgctgg atcgtcgggc cgtcttcgtc gttggcgcca ccaccgagcg tcggtgcatc
     6841 ttcgtcgtcg tcgagcgctt cctcggcggt gtcgtgttgc tctgcgctcg gcgggcccga
     6901 tgacacgacg gtcgcgtcga caccatcctc ggacgcgttg tccatcatga agccgccgaa
     6961 gcccttcggc gcgccggtga tcatcgtgct ctcgccgatg ttctcgagat caccgacgtc
     7021 gcccggctcc gtcgactcgc tgtcgtgacg cttgctccac gcaggcgcag cgagcggagg
     7081 cggcgtcttg aagctgagca ggccgctgtc ctcgccggga taatcgggcg gcagtggcgc
     7141 gggctcgacc tggcgcgtgc tctcgttctt gtcacgaggc ttcgcagtcg gcgcgcgctg
     7201 ctggccagcg gccggcgtcg gtgccttgcg cggaaccgac ggaatcgccg caggcttcgg
     7261 cggcgccgca tctttgatct cggcttgccg cgtgacgcgc ggaccggcgg gcacgggctt
     7321 cttcgctgcg tcgtgcttcg gcttgagatc gtcgacgcga aagatgacgg agttctcgtc
     7381 gtgcagctct tctttgtcga ggatcaggtc gtcggtcgag agcgtgtgcg tcgacatcgg
     7441 cccatcgcgg acctcgactt cgtagtcctc cgccggaacc tcggtcgggg caccgatgac
     7501 ctgcttgatg accgtcgaga ccttcgacgc ggtgaacacc ggggagtacg tgtgcaggaa
     7561 cctctcgagg tcggacgcga agtcgctcgc gctctggtag cgattgcgcc gatctttatc
     7621 gagcgcgtgc atcacgatct tctcgagctg gggcgggatg cccttgcgca gcgtcgaggg
     7681 tggcgcgatg tccgctttcc ggaccatatc gaggagcttg tgcagatcct cctcgagata
     7741 gagcatctgc ccggtcatca tctcgtagag cacgatgccg gcactgaaga tgtccgagcg
     7801 gtagtcgatc tggtcgcccc aggcctgctc ggggctcatg tagtagtact tgcccttgat
     7861 cacgcccacc gcggtctggc gcgccttcat cgtcgccttc gcgatgccga aatcgacgag
     7921 cttcacctcg ccggagaagc tgatgagcac gttctgcggc gacacgtcgc gatggacgat
     7981 gccgagcgtc ttgccgttgt gatcgcgctt gcgatgcgcg tgatcgagtg cgctcgcgat
     8041 ctccttcgcg acgaacgcgc agacgtcgag cggcatctcg aagtcgatct ccgagccgcg
     8101 acgcaggatc ttgtagagat ccgcgccgtc gacgtactcc atcgtgatgt agaactggtc
     8161 accgacgcgg ccgaggtcga acgtctgcgc gatgttgccg tgattgagct gcacggcgat
     8221 cttcgcttca tcgacgagca tctggatgaa ctgctcgtcc tcggcgaagt tgggatggat
     8281 catcttgagg gcgacgtact tctcgaagcc cgcgatgccc ttcgtcttgg ccaggtggat
     8341 ctcggccatg ccgcctacgg caatctgtct gactagtcga tacggcccga actgccgttc
     8401 agccacgcgg gatgtcctga tcgatagagt ccggcgtcgg cccctgcttc aacatctccg
     8461 ccccacccgg atgcacgaac acaccaacaa gacgcaacgg cgccttcgca ccgcgatcaa
     8521 acgtgcagac gacttcggtc gcgatgccgt acgcgacatt caccacgtac gtcgccgccg
     8581 cattcgcgac gagatagagg ccaagccccg cgccgtacgt cttgcggtcg atctgattgc
     8641 ccgacgtcag acacttctcg atgtacgcga ggatcgtgtt cttcgcgagc cgtccaaatc
     8701 gatcgcgcac ggccaccgcg aacatgtcct cggtcgccgc atagcgaatg ctgacgggcc
     8761 gcggcgatct cgtcttcgtg cgatcgtgcg gatcgacctc ggcgaagatc tgcttgccgt
     8821 cggcgtcgac gggcgcgtcg tagagcgcgt tcatcagcag ctcttcgcag accgtgccga
     8881 tcgcgctgcg cacctgccgc cggatcttcg attcctcggc gaagtcgagc accgtctgga
     8941 tcgccttgcc gcggccctcg aagtcgcgca gtcgcgcgta gtgcaccggc gtgttctccg
     9001 gcaggtactt ctcgatcccg aagatgtcgc cggtgagcag cttgcccgcg gtgacggaca
     9061 cgccgttctc gagatcctcg tcacccgcga tcacgtgatt gatccggtcg tctctgaggt
     9121 acttcgtcag atcaccgagc gatgctttcg gcgtgatgat cgcgacgtgc gcgcggtcgc
     9181 gcagcttcgg cacgagcgtc tgcaacgcct cgatcgaccg ttcgctcgac ctctccgcgt
     9241 gcgcgtccga cgtcgcgtag aaaatgtagc gatgtgggat cacctccgcg gcgatcgcag
     9301 acggactacg tacagcatcg acttcagcgc ccgcgcgttc gagtgtcgcg acgatgcgcc
     9361 gtagctgatc gaggtcagag gagatggcga ggacgcgccg tttcgacaaa tttccccggt
     9421 ccgcaggccc acttggccgt gcgcctcact ataccagctt acggccctca gtactgatac
     9481 agggccgtca ggtacgcgcg taacccctca tatgggtttg gcatgtactc taggtgcggt
     9541 tcgtagtcga agaacacgtc cggctggtac gtgtgcgaca tgagagcgtt gtagacggtc
     9601 gcgcgaacca gcagcttctc gatcggcgtc cacgagatgc caaccgagag gtcggcgatg
     9661 ggctggatgc gatccatgac gaggtccgtc ggattgacgg tcaccgcgcc catcgggttg
     9721 ccgttcatgt cgtacgtggc gccgcgatac tcgaccaagc ggttcgggtc ttcggccgcg
     9781 ccggtcacgc ggagattcga ggtcccggtg agcttgcccg ggatcatgtt gaagacggtc
     9841 gacaggttga accagtgctc gggcagcgag cggatgaaac cacggtcggc cgtagcgacg
     9901 cgcagccatg tgtagccgag ctcgacgcgg tggccacctt ggatgtagag cttgccgagg
     9961 aactcgcccg atgcgatgcc gcgctcgccg gagttgccgt acgagccggt gttgacctgg
    10021 atcaggttct cgacgcgcgt gtagctgccg tcgatacgga acgagagctc gcggatgcgg
    10081 cggtcgccct tgaagatacg ggcgttgacc tcgacttggg tcgcgtcgga ggtttcgacc
    10141 ttgaggtccg gcgagccggc gatgacgacc gcttcaccgt ttgccgaggt gttgttgaac
    10201 accggcgggc ggaagccttg cgtgtagttg agcttgaggt gccagttcgg aatgaagttc
    10261 cagacgatcg cgccgccgac ggtgtagttg actggatagc tgacggtgcc gagcgaatcg
    10321 ggcgcgactt gcacacgagc accggcgtcg aggatgaggc ccttggtcgg acgaaactgc
    10381 gggtcggcgt aggcaccgac gacggtgcgg gtcgcgtcgt acgcaaacgt ctgcgggcag
    10441 ccggcgacag gaacgatgac gttcgtcgtc ggatcgaacg tgcgcgggca tggaatcggc
    10501 agacgcgtca gatcgcttgg cgcgatgaag tcggcttggt tgcccgagcc ttggatcgat
    10561 gacgtcgtat cgacgggctt ccactcgcgg aacgcctctg cgccgtagag cacgcgcatc
    10621 ttcttgctga gctcgacgtc accgtcaaac gcgccgccga cgcgatcgct cgacaggttc
    10681 gtctggaagg cgaggccgcc ctggatgagc tgcgacggag cgaggacgtt gatcggttcg
    10741 aagttgcgaa cgaactgctg gtagtacgcg cgcagcgtca ggccggcttt gccgcttgcg
    10801 agccgatcgc ggtactcgcc gacgacgtag cgatcgaacg tgtcccaccg gttttcgcgc
    10861 gcggtgcccg tcggatcgtt gaggttcgcc gtgccggtcg ggaacgtgat gccgtcccag
    10921 cgcgggtcgc tgctgccgta gcgaac
//
LOCUS       DATLBZ010000204         9026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6808814, whole genome shotgun sequence.
ACCESSION   DATLBZ010000204 DATLBZ010000000
VERSION     DATLBZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6808814"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VMZ53_10610"
     CDS             <1..1182
                     /locus_tag="VMZ53_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HUS28957.1"
                     /translation="LAAWAVAAALSLFGALTYAELGAMFPRAGGEYVFLREGYGPGLG
                     YLYAWNRFWIATPGSIAAYAVGTATFLRVIVPDSPTKIVAIGLVLVFTAFNCLEVRTG
                     GWLQTVLTIMKIGMISGLAVGALFFARGGDWSRVTHDGGFPGASAFGAMVLSALWAYD
                     GWNNLPMAAGEVRDPQKNLPRAIVWGSLGVVVIYALVNVGYFHALSFGDVAAGGSISV
                     AQKTATTFLGGPAQALLAGAMAISAISAMNGSMLTGARVPYAVAADGLAPKQLAHVAA
                     GARVPTTAVLVQGVLAIGYAFIGSFDQLTDAVVFASWMFYALNAGTVLLLRKREPNRE
                     RSFRVPGYPIVPVIFMALATLLLVNAVWTAPATSALGLVVTAVGGIVYVTMYRPSVKP
                     R"
     gene            1291..1809
                     /locus_tag="VMZ53_10615"
     CDS             1291..1809
                     /locus_tag="VMZ53_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28958.1"
                     /translation="MAFGITLLGASFLLGGKDTDHGDSGHHHGDGAAAGLAWAPFGSL
                     RFWVFFFTFGGGAGFALTKMGTSSVVTAVGAGVIGWIAGALAVTVIRSMTKNSVSSEV
                     GAAELVGMTGTLTLPVAPGQPGKVRVDVKGRTEDFVANVVEDVGTLPTGSSVLIVAEG
                     ERGSLLVTKSEM"
     gene            1835..3124
                     /locus_tag="VMZ53_10620"
     CDS             1835..3124
                     /locus_tag="VMZ53_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HUS28959.1"
                     /translation="MKIPPEIVGGVVTAVIVLLVLMVFYRYLLRICRPNEILIFSGRK
                     HKTSDGRMVGYRVVFGGRGMRVPVVETVDRMDVSLISVPIAVAGAYSEGGIPLNVHAI
                     ANIKVSTDRRFIGNAIERFLGKGRQEIARVVKETLEGHLRGVLATMTPEELNQDRLKF
                     ARQLEESAKPDLEKLGLELDVLKIQHIADDRNYLESIGRQRIAEILRTAEVAESDATR
                     EAEQSEAKARARGEIAMTNAQAMLQRKQNELRQIKAEVEAEAKGEEVRAEAAGQQARA
                     EAELELQKMRGQLEQLRLEAEVTIPAEVDAQVRELFAAGQAATIAADGEAMAKSLAEV
                     AGAWRDSGGRAMDMFILQHLEQIFGDVAKATQRIKVGQVNLIDGGDGKTLPAYTASYP
                     ATMGALLTEVTRTLGIDIAKIIGGAPTNGAAPTTERS"
     gene            3127..4476
                     /locus_tag="VMZ53_10625"
     CDS             3127..4476
                     /locus_tag="VMZ53_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HUS28960.1"
                     /translation="MQNVIMVGAIGTLVALIVIGMIISRIIYICPPNEVLIFSGGHRK
                     MPGTDDRTVGYRVVQGGRGIRIPLIEVVDRMDLTNMVIELRVQGAYSRGGIPLNVQGV
                     ANVKVSSKSDHLANAIERFLSYTREQIMAVARETLEGNLRGVLSTLTPEEVNQDREKF
                     AGELVHEADHDLARLGLELDTLKIQHVSDDKGYLESIGRRQTAELLKKSRIAEAENHA
                     LSQENAASNLQNQEIAKVEAEINIARAEGQRRVAEAQTRKGALVAESRGQVQALLAKA
                     SAEVDVQKARLAQVRFQLIADKVKPAEARKAQMVLQAKGVASKIVEEGKASASAIRSL
                     AETWAKAGDSARQIFVAQKLQGLIGTMMSTVGEMPIDKITVIDKDLAANGSNFAVKAA
                     VTAEQIKQMLGVDLAGIAQRAGGAVAGATQAALPPAVPRPRPPTQPGPGTKPPGTNG"
     gene            complement(4501..6306)
                     /locus_tag="VMZ53_10630"
     CDS             complement(4501..6306)
                     /locus_tag="VMZ53_10630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28961.1"
                     /translation="MPDDRSSGTDPTLTPSQLIGEAATSADTGRSDIGRRTLDGVAAA
                     LPPRYVLGARLGAGGMGEVVLARDEQIGRDVAIKRMRVEPTPALLARFLREAKIQGRL
                     DHPAIVPVHELANDGDGKPFFVMKRLTGTTLAKILADRSLTRQKLLRAFADVCLAIEF
                     AHRRGVVHRDLKPENIMLGDFGEVYVLDWGIAHVDSETDSLHDGSVTAGEAGDTEVGA
                     ILGTPGYIAPEVVRGDAIDARADVFALGAMLFEILTGQSLLPRGRGALDATTQPFEAR
                     PSVRVPECDCAPELEAIVVAATSPQRSDRPTARELGERVEHFLDGDRDLALRKSLAAG
                     HVAAAHAALAGGDSPAERAIAMREAGRAIALDPHSGAAELVGRLMLEPPLETPPEVDE
                     AIARSEEQTARDKLRMMTFSILAFVAMIPTCLIIGVRSSTALIWLGAAIAINVLHTWY
                     VAQRSRPPMKSDMMRSAVIYAVLVFVIARDFSPFVMAPSIAAISIVMFLVDPRTPFSF
                     IVITQGAAVILPWLAELVGLAPRTISSLPNGDIVFHAVAIYASMPATEIGLALFVIGT
                     VIAAGVLARQLGMSQRAAMRTTELQAWHLRQLVKS"
     gene            complement(6355..7461)
                     /locus_tag="VMZ53_10635"
     CDS             complement(6355..7461)
                     /locus_tag="VMZ53_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HUS28962.1"
                     /translation="MPRVVTYRYIDPLTQVWVGTARKIGLRVIRTTDAYAATDGAGTL
                     SIGSNETLDRDDSLAQMIFHELCHSLVEGEESFRRPDWGMDNTGPDHDWREHACLRVQ
                     WVLTGRYGLRVLFAPTTDFRTSFWDRLSDSGSDVLEDRTERSPNGAIDVLADRTDRSV
                     IAAITALRRADHPPWGPHLAAALEATARIAREAAALSTEPDILWSGVAAAPAPHPSGL
                     PASTAASTATASCAACAWRYEQRGISRCRQASDAKIDPAWPACERFEAATAVDCQTCG
                     ACCRAAYHSVEVQKRDPVVKKQPDYIVDRGTYLEVRRDGDRCAALKGGDFVESDKLTR
                     FHCAIYDDRPRTCRDFTLGSAHCLTARRRVGLSL"
     gene            7520..7855
                     /locus_tag="VMZ53_10640"
     CDS             7520..7855
                     /locus_tag="VMZ53_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28963.1"
                     /translation="MKGDIAFAGFVLTADEWQELEPDARAQLIAVATSGPLAEGSGPF
                     QREEEVVELDVEPESDVDDGWTEPFDPPRFAAGTGVGPTPNWSAAGDIDFGPLLDPLL
                     ETRLLSKLR"
     gene            7905..>9026
                     /locus_tag="VMZ53_10645"
     CDS             7905..>9026
                     /locus_tag="VMZ53_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HUS28964.1"
                     /translation="MVTRAAPTPTTTRRGRDQARASRDPDDIRYEALLARDPRFDGVF
                     FVAVTTTGIYCRPICPVRTPMRSRCVFFETGAQAERAGFRACFRCRPELAPGASHDAR
                     ATIPGAMFDTRASRSLADVHVHGARASRSSSPAFDTRVSRSARVSVDAIDVLVAAATQ
                     RIHEGALNDGSLDDLARQLGVTARHLRRALEARIGVSPVELAQSRRLALAKQLLQDTL
                     VPATDIAFAAGFGSVRRFNAVFAARMGRPPSEVRRVAMKPTSDASKSSSNAASTTRDA
                     SKTSKSNDVTSSMRGAVGARGAVGAGAGVARGANGDVRVAGAPTLRLDYREPYDWQRI
                     LEFLRGRAIPGVEIVGDDHYRRVVHLGGHVGEIRVEPADR"
BASE COUNT         1522 a         2911 c         3087 g         1506 t
ORIGIN      
        1 ctcgccgcgt gggcggtcgc ggccgcgctc tcgctgttcg gcgcgctgac gtatgcggag
       61 ctcggcgcga tgtttccgcg cgctggcggc gagtacgtgt tcttgcgcga ggggtacggc
      121 ccggggcttg gctacctcta cgcgtggaat cggttctgga tcgcgacgcc cggatcgatt
      181 gccgcgtatg cggtcggcac cgcgacgttt ctgcgcgtga tcgtgccgga ctcgccgacg
      241 aagattgtcg cgatcggcct cgtgctggtg ttcaccgcgt tcaactgcct cgaggtgcgc
      301 acgggaggct ggctgcagac cgtgctgacg atcatgaaga tcggcatgat ctccgggctc
      361 gcggtcggcg cgctgttctt cgcgaggggc ggcgattggt cgcgcgtcac gcacgacggg
      421 ggctttcccg gcgcatcggc gtttggcgcg atggtgctgt cagcgctgtg ggcgtacgac
      481 ggctggaaca acttgccgat ggcggcgggc gaggtgcgcg acccgcagaa gaatctccct
      541 cgcgcgatcg tgtggggctc gctcggcgtc gtcgtgatct acgcgctcgt caacgtcggc
      601 tactttcatg cgctctcgtt cggcgacgtc gcagcaggcg gcagcatctc cgtcgcgcag
      661 aagaccgcga cgacgtttct gggtggacct gcgcaggcgc tgctcgcggg cgcgatggcg
      721 atctcggcga tcagcgcgat gaatggatcg atgctcaccg gcgcgcgcgt gccgtatgcc
      781 gtcgcagcgg atgggctcgc accgaagcag ctcgcgcatg ttgcggcggg tgcacgcgtg
      841 ccgacgacgg cggtgctcgt gcaaggcgtg ctggcgatcg gctacgcctt tatcggcagc
      901 ttcgatcagc tgacggatgc agtcgtgttc gcgtcgtgga tgttctacgc gctcaacgcc
      961 ggcaccgtgc tgctgctccg caagcgcgag ccgaatcgcg agcggagctt tcgagtgccg
     1021 ggctatccga tcgtgcccgt gatcttcatg gccctcgcga cgctgttgct cgtcaacgcc
     1081 gtgtggactg cgccggcgac gagcgcgctt ggccttgtcg tcacggccgt cggtggcatc
     1141 gtctacgtga cgatgtaccg cccgtctgta aaaccgcgct gacgcgttgg acattcccta
     1201 ggaaatccgc ttgcgtttgc ctgctaccga acacacattc gtggcatcga aggggcggag
     1261 gaacgattgc agcacatcta tctcggagcg atggccttcg gcatcacgtt gctcggtgcg
     1321 tcgttcttgc tcggtggcaa ggacacggac cacggcgact cgggtcatca ccatggagat
     1381 ggtgcagcag caggactcgc gtgggcgccg tttgggtcgc tccggttctg ggtgttcttc
     1441 tttacgttcg gcggcggcgc gggcttcgcg ctgacgaaga tgggcacgag ctcggtcgta
     1501 actgcggtgg gcgcgggcgt gatcggttgg atcgcaggtg cgctcgcggt gacggtgatc
     1561 cgttcgatga cgaagaacag cgtctcgagc gaagtcggcg cagccgagct cgtcgggatg
     1621 acgggaactc ttacgttgcc ggtcgctccg gggcagcccg gcaaggtccg cgtcgacgtg
     1681 aagggtagga cagaggattt tgtagcaaac gtcgtcgagg acgtcggaac gcttccgacg
     1741 ggctcgtcgg tgttgattgt tgccgaaggc gaacgcggct cgttgctcgt gaccaagtcc
     1801 gagatgtagc gttctctttc ctggggaggc tgagatgaag ataccgcccg aaatcgtcgg
     1861 tggcgtagtg actgctgtga tcgtgctgct cgtactgatg gtgttctatc ggtacctgct
     1921 gcgtatttgc cggccaaacg agatcctgat cttctcgggc cgcaagcaca aaacatccga
     1981 tggccgcatg gtcggctatc gcgtcgtgtt cggcggacgc ggcatgcgcg taccggtcgt
     2041 cgagacagtc gaccgcatgg acgtgtcgct gatcagcgtg ccgatcgccg tcgcgggtgc
     2101 gtactcggag ggcggcattc cgctcaacgt gcacgcgatc gcgaacatca aggtctcgac
     2161 ggatcgccgc ttcattggca acgcgatcga gcgcttcctc ggcaaaggcc gccaggagat
     2221 cgcgcgcgtc gtgaaggaga cgctcgaagg gcatctccgc ggcgtgctcg cgacgatgac
     2281 gcctgaagag ctcaaccagg atcgcctgaa gttcgcgcgt cagctcgaag agagcgcgaa
     2341 gcccgatctc gagaagctcg gcctcgagct cgacgtcctc aagatccagc acatcgccga
     2401 cgaccgtaac tacctcgaga gcatcggccg ccagcgcatc gccgagatcc tgcgtaccgc
     2461 cgaggtcgcc gagtccgatg cgacgcgtga agccgaacag tcggaagcga aagcgcgcgc
     2521 acgcggcgag atcgcgatga cgaacgcgca agcgatgctc cagcgcaagc agaacgagct
     2581 ccgccagatc aaggcagagg tcgaagctga agcgaagggt gaagaggttc gcgccgaggc
     2641 cgctggtcag caggcgcgtg ccgaagccga gctggagctg cagaagatgc gcggtcagct
     2701 cgagcagctt cgcctcgaag ccgaggtgac gattcccgct gaagtcgatg ctcaggtccg
     2761 cgagctgttt gccgcgggcc aggccgcgac gatcgccgcc gacggcgaag cgatggcgaa
     2821 gtcgctcgcc gaggtcgccg gtgcatggcg cgactcggga ggccgcgcga tggacatgtt
     2881 catcctgcag cacctcgagc agatcttcgg tgacgtcgcg aaggcgacgc agcgaatcaa
     2941 ggtcggccag gtcaacctga tcgacggcgg cgacggcaag acgctgcctg cttacaccgc
     3001 gtcgtatccc gcgacgatgg gcgcgctctt gaccgaggtc acgcgcacgc tcggtatcga
     3061 catcgcgaag atcatcggtg gcgcgccgac gaacggtgcc gcgccgacga cggagaggag
     3121 ctagtcatgc aaaacgtcat catggtgggt gcgattggca cgctcgtggc gctgatcgtc
     3181 atcggaatga tcatctcgcg catcatctac atctgcccgc ccaacgaagt gctgatcttc
     3241 tcgggcggcc atcgcaagat gcccgggacc gacgaccgaa cggtcggcta tcgcgtcgtc
     3301 cagggcggcc gcggcattcg catcccgctg atcgaggtcg tcgatcgcat ggacctgacg
     3361 aacatggtca tcgagcttcg cgtccaaggc gcgtactcga gaggcggcat cccgctgaac
     3421 gtgcagggcg tcgcgaacgt aaaggtctcc agcaagagcg atcacctcgc gaacgcgatc
     3481 gagcgctttc tcagctacac gcgcgagcag atcatggcgg tcgcgcgcga gacgctcgaa
     3541 ggtaacctgc gcggtgtcct ctcgacgctc acccccgaag aggtcaacca ggatcgcgag
     3601 aagttcgcgg gcgagctcgt tcacgaagcc gaccacgacc tcgcgcgtct cggcctcgag
     3661 ctcgacacgc tgaagatcca gcacgtcagc gacgacaagg gctacctcga atcgatcggc
     3721 cgccggcaga ccgccgagct gctgaagaag tcgcgcatcg ccgaggccga gaaccacgcc
     3781 ctctcgcagg agaacgcggc gtcgaacctc cagaaccagg agatcgcaaa ggtcgaggcc
     3841 gagatcaaca tcgcgcgcgc agaaggccag cgccgcgtcg ccgaggcgca gacacgcaag
     3901 ggtgcactcg tcgcagagtc gcgcggtcag gtgcaggcgc tgctcgcaaa ggcgagcgcc
     3961 gaggtcgacg tccagaaagc gcgcctcgcg caggttcggt tccagctcat cgccgacaag
     4021 gtgaagccgg ccgaagcgcg caaggcgcag atggtcctgc aggcgaaggg cgttgcatca
     4081 aagatcgtcg aggaaggcaa ggcgtctgcc tccgcgatcc gctcgctcgc cgagacgtgg
     4141 gccaaagccg gcgactccgc ccgccagatc ttcgtcgcgc agaagctgca gggcctcatc
     4201 ggcacgatga tgagcaccgt cggtgagatg ccgatcgaca agatcaccgt catcgacaaa
     4261 gacctcgcgg cgaacggctc gaacttcgcc gtgaaggcgg cggtaacggc cgagcagatc
     4321 aagcagatgc tcggcgttga cctcgcaggc atcgcgcaac gcgcgggtgg cgctgtcgcc
     4381 ggcgcgacgc aagctgcact gccgcctgcg gtgcctcgtc cgcgcccgcc gacgcagccg
     4441 ggcccgggga ccaagccgcc cggcacgaac ggctgattcg ccggactgcg aggtggggcg
     4501 ttagctcttc acgagctggc gcaggtgcca ggcctgcagc tcggtcgtgc gcatggccgc
     4561 gcgctgcgac atgccgagct gccgcgcgag aacgccggcc gcgatgacgg tcccgatgac
     4621 gaatagcgcg agaccgatct ccgtcgccgg catgctcgcg tagatggcca ccgcatgaaa
     4681 cacgatgtca ccattgggca gcgagctgat cgttcgcggc gctagcccga cgagctcggc
     4741 gagccaaggc aggatcacgg cagcgccctg ggtgatgacg atgaacgaga acggtgttct
     4801 cggatcgacg agaaacatca cgatgctgat cgctgcgatc gagggggcca tcacgaatgg
     4861 cgagaagtcg cgcgcgatca cgaagacgag gacggcatag atcacggcgg agcgcatcat
     4921 gtcgctcttc atcggcggcc ggctgcgctg cgccacgtac caggtgtgca agacattgat
     4981 cgcgatcgca gctccgagcc agatcagtgc cgtggagctc cgcactccga tgatcaaaca
     5041 cgtagggatc atcgcgacga acgcgaggat cgagaacgtc atcatccgca gcttgtcgcg
     5101 cgccgtttgt tcttcgctgc gggcgatcgc ctcatcgact tccggtggag tctcgagcgg
     5161 cggttctagc atcaagcgcc cgacgagctc cgctgcgccc gagtgaggat cgagcgcgat
     5221 cgcccgtcct gcttcgcgca tcgcgatcgc gcgctctgcg ggactgtcac cgccagcgag
     5281 cgctgcgtgc gcggccgcga catgtccggc ggcgaggctc ttgcgaagtg cgaggtcgcg
     5341 atcaccatcg agaaaatgct cgacgcgttc gccgagctcg cgtgcggttg gccggtcgct
     5401 tcgctgtggt gatgtcgcag cgacgacgat tgcttcgagc tcgggtgcgc aatcgcactc
     5461 cggcacgcgc acgctcggtc tcgcttcgaa cggttgcgtc gtcgcgtcga gagctccacg
     5521 cccgcgcggt agcaacgatt gcccggtgag gatctcgaac agcatcgcac cgagcgcgaa
     5581 cacatctgcg cgcgcatcga tggcgtcacc gcgcacgact tccggcgcga tgtacccagg
     5641 cgtgccgaga atcgcgccga cctcggtgtc tcccgcttcg cccgcagtca cgctgccgtc
     5701 gtgtagcgaa tcggtctcgc tgtcgacgtg cgcgatgccc cagtcgagca cgtacacctc
     5761 accgaagtcg ccgagcatga tgttctccgg cttcagatcg cggtgcacga cgcctcgacg
     5821 atgcgcgaac tcgatcgcga gacacacgtc ggcaaacgcg cgcagcaact tctgtcgcgt
     5881 cagcgaacgg tcggcgagga tcttcgcgag cgttgtgccg gtcagccgct tcatcacgaa
     5941 gaacggtttg ccgtcgccgt cgtttgcgag ctcgtgcacc ggcacgatcg cgggatggtc
     6001 gagccgcccc tgaatctttg cctcgcgaag gaagcgtgcg agcaacgccg gtgttggctc
     6061 gacgcgcatg cgcttgatcg cgacatcgcg gccgatctgc tcgtcacgcg cgagcacgac
     6121 ctcgcccatg ccgcccgcac cgagccgcgc gccgagcacg tatcgcggtg gcaacgcagc
     6181 ggcgacgccg tcgagcgtcc ttcgccctat gtcacttcga ccggtatccg cgctcgtcgc
     6241 ggcttcaccg atcagctggc tcggggtcaa cgtcggatcg gtgccgctgc tccggtcgtc
     6301 gggcatagcg cgagcttaac cgctcggcac ggccagcccg aattgctgca catctcagag
     6361 cgagagaccg acgcgacggc gggccgtcaa gcaatgcgcc gagccaagcg tgaagtcgcg
     6421 acaggtacgc gggcggtcgt catagattgc gcagtgaaag cgcgtgagct tgtcgctctc
     6481 gacaaagtcg ccacccttca gcgctgcaca tcgatcgcca tcgcgccgca cctcgaggta
     6541 cgtcccacga tccacgatgt agtccggctg cttcttcacg accgggtcgc gcttctgcac
     6601 ttcgacggag tgatacgcgg cgcggcaaca tgcgccgcac gtctggcagt cgaccgctgt
     6661 cgccgcctcg aagcgctcgc acgccggcca cgctggatcg atcttcgcgt cggacgcttg
     6721 ccggcatcgc gagatgccac gctgctcgta gcgccacgcg cacgccgcgc agctcgcggt
     6781 cgctgtggat gcggcggtcg atgccggtaa gccgctcggg tgaggcgcgg gtgccgcggc
     6841 gacgccggac cacaggatgt cgggctccgt cgacagcgcg gccgcttcac gtgcgatgcg
     6901 cgcagtcgct tcgagcgcag cagcgagatg cgggccccac ggtgggtggt cagcgcggcg
     6961 cagcgcggtg atcgccgcga tgaccgatcg gtcggtgcga tctgcgagca catcgatcgc
     7021 gccgttcggt gatcgctcgg tgcgatcttc caacacatcg gatccgctgt cggacaggcg
     7081 atcccagaag ctcgtgcgga agtcggtcgt cggcgcgaac aggacgcgca ggccgtaccg
     7141 accggtcagc acccattgga cgcgcaggca cgcgtgctcg cgccagtcgt ggtcgggacc
     7201 tgtgttgtcc atgccccaat cgggccgacg gaacgactcc tccccctcga cgagggagtg
     7261 gcagagctcg tggaagatca tctgcgcgag cgagtcgtcg cggtcgagcg tttcgttcga
     7321 gccgatcgag agcgttccgg caccgtctgt cgccgcgtac gcgtcggtcg tccggatcac
     7381 tcgcaggccg attttgcgcg cggtcccgac ccacacttgt gtaagtggat cgatgtaccg
     7441 atatgtgacc acgcgtggca cgcgctgaca gctagcacga aaagttgccg ctgcgtcgag
     7501 agattcagat ggtggagata tgaagggtga catcgcgttt gccggtttcg tgctcaccgc
     7561 tgacgaatgg caggagctcg agcccgatgc gcgcgcgcag ttgattgcag tggctaccag
     7621 cggcccgctc gcggaaggca gtggaccttt ccagcgcgaa gaagaggtgg tcgagctcga
     7681 tgtcgagccg gagtccgacg tcgatgacgg ttggacggag ccgttcgacc cgccgcggtt
     7741 cgccgcgggc acgggcgtcg gtccaacgcc gaactggagc gcggccggcg atatcgattt
     7801 cggcccattg ctcgatccgc tgctcgaaac gcgtctgttg tcgaaattgc gctgaggtcc
     7861 ggaaacggcc agtcagtgtt ggggcggcgt ggtacctcca tgtcgtggtc acgagagcag
     7921 cccccacacc gacgacgacg cgccgcggcc gagatcaggc acgcgcgtcg cgtgacccgg
     7981 acgacatccg gtacgaggcg ctgctcgcgc gcgacccgcg gttcgacggc gtgttcttcg
     8041 tcgcggtcac gacgactggc atctactgcc ggccgatctg cccggtgcgc acgccgatgc
     8101 gatcgcgctg cgtcttcttc gagacaggag cgcaagcgga gcgcgccgga ttccgcgcct
     8161 gcttccgctg ccgtcccgag cttgcgccgg gtgcctcgca cgacgcgcgc gcgacgatcc
     8221 ccggcgcgat gttcgacacg agagcatcgc gctccctggc ggatgtgcac gtgcacggtg
     8281 cgcgagcatc gcgatcgtcg agccccgcgt tcgacacgcg agtctcgcga tccgcgcgcg
     8341 tctcggtcga cgcgatcgac gtgcttgtcg cggcggccac gcaacgcatc cacgaaggtg
     8401 cgctgaacga cggctcgctc gacgacctcg cgcggcagct cggcgtcacc gcacgccacc
     8461 tgcggcgcgc gctggaagcg cgcatcggcg tgtcgcccgt cgagctcgcg cagagtcgtc
     8521 gcctcgcgct cgccaaacag ctgttgcagg acacgctcgt gcccgcgacg gacatcgcat
     8581 tcgccgctgg cttcggcagc gtgcggcggt tcaatgccgt gttcgccgcg cgtatgggcc
     8641 gaccgccgtc cgaagttcgc cgcgtcgcga tgaaaccgac aagcgatgcg tcgaaatcgt
     8701 cgagcaatgc agcgagcacg acacgcgatg cgtcgaaaac atcgaaatcg aacgatgtga
     8761 cgagctcgat gaggggcgcg gtcggcgcgc gcggcgcggt cggcgcgggc gcgggtgttg
     8821 cgcgcggcgc gaacggcgac gtgcgcgtcg ctggcgcgcc cacactgcgc ctcgactatc
     8881 gcgagccgta cgactggcag cgcatcctcg agttcttgcg tggccgcgcg attcccggcg
     8941 tcgagatcgt cggcgacgac cactaccggc gcgtcgtgca tctcggtggg cacgtcggcg
     9001 agattcgcgt cgagcccgcg gaccgt
//
LOCUS       DATLBZ010000205        29699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7552700, whole genome shotgun sequence.
ACCESSION   DATLBZ010000205 DATLBZ010000000
VERSION     DATLBZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 29699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29699
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7552700"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1553)
                     /locus_tag="VMZ53_10650"
     CDS             complement(159..1553)
                     /locus_tag="VMZ53_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28965.1"
                     /translation="MTDIYGQILSKALFPVFEAARGRPTVPLLKYLEETSTWSAPELR
                     ELQTGFLRRLLRHAYKHTAYYRQLLDERGLVPEDFTSPADLVKLPLLDRPTLRATMKE
                     RTADAPPVAVIRKVTSGSSGQPVEVLYNHESRHWRDAIRLRGYGWGGYDIAKRSMHYW
                     GFVPMSSSTWWKRQKMTLDRAYKRELYLDCTPRSEEALMNVVNQIRRFKPQVMVAYAS
                     GAGALARFVNDRNLRDWQDIPVLTGAEGLLPHDRKEIDKAFGAAFDTYGCREVMLMAS
                     ECEAHEGMHTSMENMIVEVIVREPGGTIRAAKPGETGEVVITDLHNLACPMIRYVNGD
                     LAVAGSDERCKCGRGLSKIREIQGRVTETMYDGQGNAVGGLVFNILFSTIGHVARSFQ
                     VVQKPDASVIFKVVPFEGRDLPSKEESVLRAHAERYLPGVPFSIELVDDIPLTSAGKR
                     RVVVCEMQQPRRSA"
     gene            complement(1550..2926)
                     /locus_tag="VMZ53_10655"
     CDS             complement(1550..2926)
                     /locus_tag="VMZ53_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28966.1"
                     /translation="MDLYGPLLSKVLFPAFEAARGRPTVPLLGYLQQTERWSLDALRD
                     LQVGFLRRLLRHAYQHTAYYREVMDDRGIRPEDIESVEDLHKFPLLDRDLLRKTIDTR
                     LAGAPPKWVITKSTSGTTGEPVVVRYNHESRHWRDATRLRGYGWGGYRLGVRAMHYWG
                     FGPPAKTWTHRRKIEIDRWVKRDLFVDCTPRGEEALTAAVEQIRDFKPQVMVAYAAGA
                     AALAKFVNENKLRTWKSFPVLVGAERLWPHDREAIEEAFGDAFETYGCREVMLMASEC
                     EVHAGMHTSMETMIVEVVVRNPDGTTRAAKPGETGEVLITDLHNLACPMIRYVNGDLA
                     VAHEPTVCACGRSLTKIGPIEGRTTETLRDGRGNAVGGLVFNILFGVMDQVASKFQVV
                     QKIDGSVIMKVVPTAGPKLPDANYRAIHAFADKYLPGAPFNIEYVEDIPLTAAGKRKV
                     VVVERPSP"
     gene            2957..4051
                     /locus_tag="VMZ53_10660"
     CDS             2957..4051
                     /locus_tag="VMZ53_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28967.1"
                     /translation="MLSRLRKKNLHQWLPDYARHLVRRARTPQSGNHRHLLFALCDHY
                     EPHWGKAPDDVAKARVDAWADNYPKLGEFRDSDGRAPRHGFFFPGEEYKPYYLDRLGE
                     LARAGFGEVEFHLHHDGDTAETLAPRIREHIKTFSEHGHISRDKAGNFQWAFIHGNWC
                     LANGRPDGRWCGVDDELPLLKKLGCYVDLTFPAAPDPSQPDKVNQIYWPVGDLNKRRS
                     YDHGERARVGHSYDDRLLMITGPLAFARKGTVGIRMENGALTGDDPPNAQRVATWVNQ
                     GIHIEGRPEWVFVKVHTHGAIEKTAASLLGDGAHAMHTALQQYKRNGWQLHYVTAREM
                     FNVARAAMDGKNGSPADYFDYLVKPPPVAQ"
     gene            4094..5470
                     /locus_tag="VMZ53_10665"
     CDS             4094..5470
                     /locus_tag="VMZ53_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28968.1"
                     /translation="MRRSVGRAGLIATSVIAAVQLQQACKFDHGALVTGMPDGGPDAL
                     PDASECTAAMDMCLGDTLRHCDGPGAMAVDRPCGWGCMTNRCLEVVPAGSGGNASDGV
                     MPSDVVAVDMTTAVELVEGLIVDGDNGRIGTTMNPTSLRGSGSGVIAGIDWQMRGPVS
                     MFRFKSLKITTSSAGSVALIGSHPIAFVADEGVEVDGIVDARGTCAGSAAGPGGFRGG
                     AIDTSATGSGAGKAVNASSVGGGGGGHGDDGGDGGNSGGQGGQSFGGPAIALLLGGGG
                     GGGGHGGGGGTGPGGGGGGALQIVSNGLVSILSGGINAGGCGGRTGTGGSDGGGGGGA
                     GGTILIEAPKVNLEGGVLAVNGGGGGGGAGTTATTGTSGQLSEAAAPGATNGADGGAG
                     GAADKLDGTSGETSGGDGGGGGGGVGRIRINTRDNMGILPAIIGTITSPRLSDATTTT
                     TRGSAATR"
     gene            complement(5467..6372)
                     /locus_tag="VMZ53_10670"
     CDS             complement(5467..6372)
                     /locus_tag="VMZ53_10670"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HUS28969.1"
                     /translation="MESVEFTGAQADKPRWRENLPFWGVHVAALVGAIIVGWSWTAFW
                     WLIGTYAVRMFAITAGFHRYFSHRTFKTSRVFQFILALLGMTTAQQGPLWWAAHHRRH
                     HKYSDMPEDIHSPRQRGFIWSHMQWILAKRFKATDYDRIKDFAKYPELRWLNRHDLIC
                     VIGTAVITFAIGGTTALVWGFFVSLIFSWHLTFCINSLAHVLGSRRYETTDDSRNNLA
                     LALLTFGEGWHNNHHHYQRSARQGFYWYEIDISWYTLKALQALRIVWDVEGVPRHVRD
                     QTEAPERARVQSGGSASSVPARQAD"
     gene            6574..7047
                     /locus_tag="VMZ53_10675"
     CDS             6574..7047
                     /locus_tag="VMZ53_10675"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HUS28970.1"
                     /translation="MLAAYFDASHQLEQDRAIDIVEREIIELVDDGDTIRRTSRVVPR
                     RQLPGFLRTLSSGPLHYLEVATWHRAANRISIEIRPSLMGGRAQICAEYQLDVVAPNT
                     IRRRYAGHVSVDVALLRARVERGIVAEFERSMPLAASCTQAWLDRQSPTSVSART"
     gene            7079..8806
                     /locus_tag="VMZ53_10680"
     CDS             7079..8806
                     /locus_tag="VMZ53_10680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS28971.1"
                     /translation="MTIPAGTLVPRDARDFGSYQLLAKIATGGMAEIYLARPAASQST
                     KELMVLKRILPHLAEDEHFVTMFRDEADLAAKLVHKNVCNVLSFGQFAGTWFIAMEYL
                     HGVPLSRMMTKLSKAQKMLDFRMVAGIICQACEGLHSAHEARDEKGQLLGVVHRDVSP
                     PNIMVTADGTVKLLDFGIAKARGANSRTRTGTVKGKNAYMSPEQILGKPLDRRSDIFA
                     LGVVMYEMLAIKRLFHRDSDFLTFKAITEEAIPDIRDRRPDLPPGMRAALLQAMARDP
                     AGRFDTAQGFGNAIKNSVATLGGPASPADLARLLFTDFSDEMSSRDEILKAADDPNAS
                     PISISTKPAVPPPIPKREVQLATSGEMHQATPATGRSRTPTGNLEPGVIIASSHGAQT
                     AKITMPDLANVLDLSEDVGADQWMAEGGTDLLSSHRWRSLRNLALLLIGIAVVGVGIY
                     MFFKTTSDGSDTKVVTAPPIDAAVVRKNDPPPLDAPEVNKNDIIALSKFGFFTIDASA
                     KTTIYVDNVRYGETPMQRLPLTPGPHKVKGVTKGKKPREFMITIIGGRDTELETIDWE
                     GPAPPPLPK"
     gene            complement(8823..9119)
                     /locus_tag="VMZ53_10685"
     CDS             complement(8823..9119)
                     /locus_tag="VMZ53_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28972.1"
                     /translation="MASDAGLRVVGRILTIELHTLVARFEHVTSRQVAKIQLDIERAI
                     DEEGEELDVHNLADLHFQGPAELVPRFHAGDRVQIVTSPESSLQISSIRPAPLS"
     gene            complement(9151..9756)
                     /locus_tag="VMZ53_10690"
     CDS             complement(9151..9756)
                     /locus_tag="VMZ53_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUS28973.1"
                     /translation="MTAPANIHELLPLYAINALAADEAADVERAVQADPALAAELDSY
                     LALIAPVEPSPDVKARLLASAGGGRFDRFADRMAKIFDVTVDRARELLGLVERAASWE
                     TPMPGISLIHFDGGPACATADCGFVRIQPGCTFPWHTHRGEEINVILTGTLRDSTGKV
                     LQAGDELVQQASTQHDLTAGDEEVIYVARATDGIEVSGPRS"
     gene            complement(9753..10292)
                     /locus_tag="VMZ53_10695"
     CDS             complement(9753..10292)
                     /locus_tag="VMZ53_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS28974.1"
                     /translation="MDPGDDAEDAALVAAMAKGDRSALAAIYERHSGVLLAIALKIVR
                     ERREAEDLLHDVLLEAWRAAKDFDPKRGRVRTWLAIRMRSRALDLQKSARVSRNAGDA
                     GLEVVVDNSERSSPDHARVRTALDGLSGDQKRVVELAYFEGLSCSEIAERIAIPIGTV
                     KSRLAAGLQKLRDTLGGTR"
     gene            10323..13214
                     /locus_tag="VMZ53_10700"
     CDS             10323..13214
                     /locus_tag="VMZ53_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS28975.1"
                     /translation="MSLREAVRAEATDRTWSQGVTLAREGRVVGIGRSDEEVELEVRV
                     PGRPTPFEVVLDLENDEWQCNCPSKEAVCSHVVAAVLANEQAGDALPTSSKVGAAIRY
                     LLEPDPGGVKVERMLVRDGNSEPLVGSLMSLVGKGKASHIATQETDLLADQLLGVRSV
                     VSGEKLDRLLEALADARDVRWRGDPVTTNGEPVLPRAIVEDFADGVRVRLERDPVVRE
                     VVAIGLARTEDNVLRPIGAVDLGGTRFEKLPQSFDVPRSAIPELTGKTLPALSQRISV
                     VVKSSTLPQVGKREEPRMQIDVEQDGDKLIVMPTLVYGDPPRARIDGGRTLVHLGGTL
                     PIRDEDAERRLLHRLRDELNMVPGRRVELTGRDAFAMQQHLGNWLRSDAKGAATSKAV
                     QLELRIAIDGDRLDVDLEGSGGKSASVGAALRAWQNGVDVVPLSGGGWGRVPMDWFDK
                     HGERLADLLATRTSDRRVPIYSLPDLARLCEDLDQPPPPELERLKPLLENFDGLRKAK
                     LPKGFQGELRPYQQSGLDWLAFCREAGLGCVLADDMGLGKTIQALAAINGKTLVVTPK
                     SVLFNWLAEAEKFRPDLKVTTYAGTRRTLDTSADIVLTSYPILRNDIDDLAETTWDTI
                     ILDESQTIKNPDSQVARAAYRLKGNWRITLSGTPVENRLDELWSQLHFTNPGLLGGRA
                     DFQERWAEPINLGDNAAAARLRDRIRPFVLRRMKSEVAKELPPRTDSIMYVELDENER
                     TTYDAIRAATQREIVKLLESGGGIMAALEALLRLRQASCHLALLPNGMRTGETPQSSS
                     KLERLMEALADAVADGHRALVFSQWTSLLDLIEPHLRAADTKFVRLDGSTADRGAVVN
                     EFQADDGPPVMLLSLKAGGTGLNLTAADHVFLVDPWWNPAVEDQAADRTHRIGQDKPV
                     MVYRMVARDTVEERILELQERKRRLANAALSEAGGATSITREDLLALLS"
     gene            complement(13211..13978)
                     /locus_tag="VMZ53_10705"
     CDS             complement(13211..13978)
                     /locus_tag="VMZ53_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS28976.1"
                     /translation="MRGALVAVGIVAGCGRLGFDAGSTPDARAVTTYRDAVLDDHPTA
                     YWRLGTPAPGRDEMGQFDATFKGTCAAVQGPLNADADTALHFNGSSCFAVIPSGLEFP
                     NRAPFTVEAWIRDERVDSYQIYFIKETRMGNGPLDGYALLINSNGVYLERIVNTGGQV
                     TNPTPIAANQWIHVVGTYDGAVVRLYFDGVQVGPAKTAPDTMPAVPMESSIGAYPAGN
                     TFLQGDMDELAIYDYALSPQQIVKHHDIGINGPLVAN"
     gene            complement(13991..14317)
                     /locus_tag="VMZ53_10710"
     CDS             complement(13991..14317)
                     /locus_tag="VMZ53_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28977.1"
                     /translation="MAEDVSMQQLVELLPARIWYLTSNGQDMWCRRPYGFLFSDGQSA
                     ESFAKAMGTGEELFAVGCDAGAFMSDEMLAGLRNSAVTRIFIDPAIDADTGDVHGKIL
                     RLAPLT"
     gene            14343..15245
                     /locus_tag="VMZ53_10715"
     CDS             14343..15245
                     /locus_tag="VMZ53_10715"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HUS28978.1"
                     /translation="MSAPTGVPREAKRVQPPHADYAFWVIGAKRGVLRDAYHTFLRVR
                     WSIALALIALGLVLANVFFAAIYYVIGGVKSADDSFFEMFSFSVQTMSTIGYGTEYPL
                     STAATSVMISEAISGIILTALATGLVFAKFARATGRIAFTKHAVVTMHDGKPTLMFRC
                     GNERSNVIVEAQLHVTAAMVTMTAEGRPFYKIHDLELVRSRMSGLRRGWTVMHIIDET
                     SPFHGKTAADLAKAECEIEISLTGIDDVTMSTIHTLHVYSDKAIKFGHRFADTIKPLA
                     NGDLFLDLTQFDVLLPDEHASVPA"
     gene            15242..15715
                     /locus_tag="VMZ53_10720"
     CDS             15242..15715
                     /locus_tag="VMZ53_10720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUS28979.1"
                     /translation="MIRLAALVLFLVACSGSPGGPNMNNRMAGPDPVAQSSDVISADI
                     LAREPVANNAKVKHVLIGWEGMDGSDERAKKRTKADAEALVNSLMTQIKAGANFEMLM
                     KQHSEDPGSIGGNSYDVSPDAGLVIEFKQLSLRLNVNEVGVVQTNYGFHIIKRVE"
     gene            15712..16401
                     /locus_tag="VMZ53_10725"
     CDS             15712..16401
                     /locus_tag="VMZ53_10725"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28980.1"
                     /translation="MTSLAIILGLLVGARHAFEPDHLAAISTLVTSPRSPRSASISTR
                     SGLFLGLLWGLGHTIALLGVGVALVLAGGLLPTRIGNAFELAVAGMLVILGARAIVRG
                     VRNKEGRTGVHHHGGVAHAHAGAHDHVHLGDRAYAWRPLTIGIVHGLAGSGALTALAF
                     AELPTTSARIIYMLLFGIGSVAGMTLATGVTGTVLARLSRGDNAQRVLAIATGSVSCI
                     CGVLWALPLLA"
     gene            complement(16402..16845)
                     /locus_tag="VMZ53_10730"
     CDS             complement(16402..16845)
                     /locus_tag="VMZ53_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28981.1"
                     /translation="MKYLLLALSLAACGDHPTSTGTVCPSPDPMTLTYDNFGKPFMEK
                     YCNWCHESTLPRSQRNGAPLFHDFDSLLGILQVQGHIDQQAGIGPNASNGFMPPAECP
                     SVIGGKLDGNCAQPTEEERRNLALWLACEKDRPHNFGADAGVDSP"
     gene            complement(16846..17610)
                     /locus_tag="VMZ53_10735"
     CDS             complement(16846..17610)
                     /locus_tag="VMZ53_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28982.1"
                     /translation="MVLRIVVFLALASAPAFAQHHHGMESHSEEPRDVFGAGVAFVAA
                     SFETMDYVGNYEGVLPSVRWSRPRFGAGVTAGFYRLEKNGADYYDVGDLMVNGQATLV
                     GDSHASAGVTAAVMTPIGAGAHGTSMGHVMLMPALFGAYQVDRFDVVATAGYSRAIGG
                     HTDHDHGPWPLVEPMLMSELSWSAGGDLRATNDVRVGGRVSGGIPVGDAGSTRVVGAL
                     RIGWHSGRVDTTAEVQAGLVGDPFKVRGVVSTALSF"
     gene            complement(17631..17933)
                     /locus_tag="VMZ53_10740"
     CDS             complement(17631..17933)
                     /locus_tag="VMZ53_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28983.1"
                     /translation="MRFALAILSTAFLFAACGGDEEESYDTYKACFDDHHNVESLPVD
                     QAIVVCCIEHEIGGAAKNTLCGGDSAACTTYVTAQLGQSATTAEISAGCTTYQSQH"
     gene            complement(17992..18333)
                     /locus_tag="VMZ53_10745"
     CDS             complement(17992..18333)
                     /locus_tag="VMZ53_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28984.1"
                     /translation="MSLRALLTAATTVIVAIALAVLVPVAQLQTFVEKDACCCPNPKA
                     CKCNHDDPAQPTMKTCHQKAPAIASTTLPSFVPPEVTDVVAPSRALIAINAPLPDPHP
                     APAPRRPDAPS"
     gene            18435..19553
                     /locus_tag="VMZ53_10750"
     CDS             18435..19553
                     /locus_tag="VMZ53_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28985.1"
                     /translation="MLFLAAQTALLWWVAARDRTTEAHFAWTTTVVAPPAASPPAPVV
                     LAATPPATTRPSCPPPRTDAATRKRNLPTGVNHVTTSVTNAGWLAAWDEHRVLVSTDG
                     GISFTQMLDGPGTVNDVTFDCFGHPIVSRGDKLGIRDGTREMWRTVSGLRFEDYAYPL
                     LIGGGPDIVIAGSEVNDESHTLLAISSDLGATWWFKDTGVYWDSTRAVGRQDANGTIH
                     IAFTTADCMSDPTTWVRVTPDGTLASDDLGDIGRIGLYGDIAIATYGDTGGVGWKRFG
                     EKQWHEVKGLADTDHTRVELVDGPLARIVSNGVIYTVDARGNAKALRPWPYGTASVDR
                     AGQLWGIDETKDGEDAWLVAVPGTKAPIPPPSEWNGNE"
     gene            complement(19554..20879)
                     /locus_tag="VMZ53_10755"
     CDS             complement(19554..20879)
                     /locus_tag="VMZ53_10755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUS28986.1"
                     /translation="MAAFELDAVVLATDELSLVRIAGLTARERASRVAAKVGATRVHV
                     VERVDDDELARWRAGRSCPLLVIRADQLVHTPLVAPLIEALPTLDRDGVAIAVVPDAP
                     VVQDLPPGSYAGALLATGRGADDVIAGLADDTALVARLVEPRRVPHGPIARAPIATAE
                     QRRQAHRVLYRILIKPQDNAITRYLYRPVSFPLTRLLVWTPITPNQISYVVAVLVALG
                     CWLTAHASFNMVLLGTIVVLAASYVDCCDGEVARVKLLSSRFGAWIDTVVDELSSVGY
                     MVALGWHCHEHFGPSFFGELPVDPWKLAIAISVVTYLWSIYCVYYNIIVAVGSANSQD
                     YVGRFEVVPGSAPNTVRLAPAAAKAIATKEPLPPWLDFIATYAPYIVRRDFIAWASVL
                     LAALHVTHLSFGLLAAGGLVSAAILTIDHLKLRSLRRSIERRGLLLDAP"
     gene            21072..21839
                     /locus_tag="VMZ53_10760"
     CDS             21072..21839
                     /locus_tag="VMZ53_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HUS28987.1"
                     /translation="MKAVILAAGCATRLRPHSDDRPKTLLTVGGVPILRRTITSLLRC
                     GFDQFVIGTGYLEHMVREAVVSWFPTLDVTFVTNPDYRTTNNGYSLMLCAPHVENDAF
                     ILLDGDVVFDIRVVEQLVEHGPDCLAVRSVGGIGLEEVKVTADSNDRVLAIGKHVPVR
                     SAMGESVGIELFSASSSKRLFAALDKRVRQQGLINEYYEASFQQIIDEGSTLYGVDMG
                     SLYATEIDTIDDLLAANARLAAQPAFDVSVDAVRLAV"
     gene            21939..22601
                     /locus_tag="VMZ53_10765"
     CDS             21939..22601
                     /locus_tag="VMZ53_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28988.1"
                     /translation="MGCASAETGERSSSLQPDAGPGGGGGQMDAPGTGSGSGSGSGSG
                     SGSGSGSNGCTTSDLLVNGNFDGGATGWTATPVSTDPIIGPVTTPAPQSGANIAWMGG
                     YAKAAPNTDSLYQDVAVPAGTTMLAFTGYYYINTEELGGMYDTGTVALQTTTGTQIEA
                     IQALSNGNTTTTWTAINHPFTNLASIKGQTVRVKVTTNSDSTFRTNFMFDTFALTATV
                     CP"
     gene            22674..23339
                     /locus_tag="VMZ53_10770"
     CDS             22674..23339
                     /locus_tag="VMZ53_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28989.1"
                     /translation="MRFAVLVVLAACGPTEVTSPTDNLGGAGTGESFESDPYPVKRVR
                     LEQTTGDNTLVAGLSLACSDPNGTKENSWYRIFALKDFGVDKPFQVNRVNFGVQTAIG
                     EQRVKVSIGTYAGNAGSVELDTSKIDMLGLTTIAVPEGKLEMMQASFPSIAVPADANL
                     VVEIKTEGRGDGAFFYLGATESPEVMPGYLRAPGCSTDRPLMTSALGYSQTHLLISVS
                     GAY"
     gene            23355..24113
                     /locus_tag="VMZ53_10775"
     CDS             23355..24113
                     /locus_tag="VMZ53_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28990.1"
                     /translation="MRRALLLVVAGCAQGGRDVSIDASTGLVDAPKQQMDAPAVDAPP
                     GTQTRELTQTTSQTIQPQSSIACANNPPGTNSNNYYRVFDLAALGITSPFKVTEVTFQ
                     IEHSDQINGTAGSTVAVRVGTYAGTPSDVLELSMMTVLASNPTVAVPEVISNPGPPPT
                     TPGGTVHAPISATIPAGQKLFIEVDSPDGHNQYYFYMGANTAGETAPGYVLAPTCVDG
                     NQMPISKPTNISKVAPSVYPKVNLLLSVTGQYQP"
     gene            complement(24085..24723)
                     /locus_tag="VMZ53_10780"
     CDS             complement(24085..24723)
                     /locus_tag="VMZ53_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28991.1"
                     /translation="MDARTAEVRRVLDRYLIEVVEKYELCPWAKPARLGDEVAIEVLW
                     GIPTIDAWSEAARALLARPKTRVAMVLAPEIEAPPAELRVIRNRVNSRLPGVGIAEFH
                     PDGITDTATAARLVPFLRRSPDPLLQLVPLSLLESVRGAPQIVDVMEQAQILSGQMAP
                     PRGDVADELAEQNHARVTARMAEVEGVLASIAEDRRTSYARVGITVGTGRSR"
     gene            24763..25776
                     /locus_tag="VMZ53_10785"
     CDS             24763..25776
                     /locus_tag="VMZ53_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28992.1"
                     /translation="MKAFITAILLCSTASAWADDVASERCAKGVELAAKNDLPRASLY
                     LDGCTDEASSDAAARVAKKIRASDLAALSISTTPDGLTAETDALPGETFTTPATIYAK
                     PGTYSVTVQGLTQKVTLAARSRGTVIIDARVKTQAPRDGKADFSEDTPEQTAHKGPPP
                     DVKRGSIVPKKYLGIPDDPSGPEIDDPFAYRDSHLAWRFGVRVGGGFMSQGDSGVGVS
                     VAALASRPLDGPAFLAARVDYNHRTLDTIGVNAGVSVKLVETASVVLLAGGALRGEIH
                     MQDTLAMQPVARATLGGAADLDLAILSLPVALGLRYEQGFTELLSGSRDRAVLLEAGY
                     DWR"
     gene            complement(25773..26684)
                     /locus_tag="VMZ53_10790"
     CDS             complement(25773..26684)
                     /locus_tag="VMZ53_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HUS28993.1"
                     /translation="MRSRFAPSPTGEAHPGTLLSALLVWLDARARAGQALLRLEDLDT
                     TRVKAAWSEQMTDALAWLGLDWDESVVQSGRTRAHEAALDRLTERLYPCTCSRADRAG
                     GRRAPDGGWAYENTCRDKPLPAGGWRSVSDNLRVRLPDDRVDLVDDGGMHLSQTPARD
                     MGDPIVRRRDGVIAYQLAVVVDDADTAITNVVRGHDIAPSTATQVLLQRLLGVPTPRY
                     RHHFLLLEHGKNEKLAKLHGSIPFGELRARHRADELCGLLAHAANLIDAPKACTPREL
                     IETFDWSRVPREDRVATFDRVRGLVIV"
     gene            complement(26703..27308)
                     /locus_tag="VMZ53_10795"
     CDS             complement(26703..27308)
                     /locus_tag="VMZ53_10795"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HUS28994.1"
                     /translation="MAGWTAAASPVPLPAQAVATMFAGEVSAPIPVSLYDENDHQKGT
                     VAIWRDGSTDEATSTEIKKLFRCRKTHREHTMKQQTLAMIADIAERYAGKTIEYVSAY
                     RVGRDESATSPHRRATALDFRIRGIQLREIRDYLWKTYAHVGVGWYPEGQYIHIDSRP
                     ELNDTSWTFLHGSNHYDPTWASLARRPEQVAASHDHHRPGS"
     gene            27532..28692
                     /locus_tag="VMZ53_10800"
     CDS             27532..28692
                     /locus_tag="VMZ53_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS28995.1"
                     /translation="MRFTEEHQQLRRTVRDFVEKEINPHVDKWEREGAFPAHEVMKKA
                     GRLGLLGVNKPEAYGGLGLDYSYQMVVIEEMGTCASGAVPMAMGVLTDMATPALARWG
                     THELKQEFLAPVIAGEQIASIAVSEVSGGSDVAALKTNARKDGDDYIINGSKMWITNA
                     AQADWFCTLVNTSEGAPHANKSLVIIPAKAKGVEIGAKIDKLGMRASDTCPVFFENVR
                     VPQRNRIGQEGTGFMMQMVQFQEERLYGAVSTLRGMEGVIDATIAYCKERTTFGQPLI
                     DNQVIHFRMAELKTEVEALRALSYRAVEDYVGGADVTMLASMAKLKAGRLAREVADSC
                     LQYWGGMGFTWDNVAARAFRDTRLLSIGGGADEIMLGIICKLMGILPGKKKR"
     gene            complement(28695..>29699)
                     /locus_tag="VMZ53_10805"
     CDS             complement(28695..>29699)
                     /locus_tag="VMZ53_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28996.1"
                     /translation="GAVFGVELVRFGHMSIIADDGSRPWHEMVPTPQARHGGPNGETE
                     RRVQAPTIQPPMASAAPESSLLETVRANKPAGLVIPPPPTPPGIAQSRTKTNTAPPPI
                     PTAAKRPQSQPSPDGRPGEASGEIQVKNKSTMQGHSGAPKITPAGPVPAPIPPIPSLP
                     SSGLEATPNPQRVITTPAPGSVPAVPSPRRAGSMEVQSTDEPTQPGVDIVRGAGGGAS
                     LGGGVVGGEIDSNKKKIPTASSEKKTDPEPVQAEPRGSILGIGMGAIGSTSVGVAKGV
                     ASGEISQKIAPGGRVLVPGPNGLMQSATVRNLVQGYYELEVGGSGETIWVPISGVVPE
                     "
BASE COUNT         5253 a         9867 c         9623 g         4956 t
ORIGIN      
        1 gatgccgact tcgccgagct cgttgacgat gatgccgagc tttcccgtag cggctcgttc
       61 agcacgtcgc gcgagcagcc ggttcacgag cgtggtcttg cccgcgccga gaaagccagt
      121 cacgacgacg agcggaacgc gcacacgagc agcctaccct acgcggagcg gcgcggctgc
      181 tgcatctcgc agacgacgac gcggcgcttg cctgcagacg tcagcgggat gtcatcgacg
      241 agctcgatcg agaacggcac gcccggcaga taccgctcgg cgtgtgcgcg caagacgctc
      301 tcctctttcg acggcaggtc gcgaccctcg aacgggacga ccttgaagat cacgctcgcg
      361 tcgggtttct gcacgacttg gaagctgcgc gcgacgtgac cgatcgtcga gaacaggatg
      421 ttgaacacga ggccgccgac ggcgttgcct tgcccgtcgt acatcgtctc ggtcacgcgg
      481 ccttggatct cgcgaatctt cgagaggccg cggccacact tgcagcgctc gtcactgccg
      541 gcgacggcaa ggtcgccgtt gacgtagcga atcatcgggc aagccaggtt gtgcaggtcg
      601 gtgatcacga cctcgccggt ctcgccgggc ttcgcggcgc gaatcgtgcc gccgggctcg
      661 cggacgatga cctcgacgat catgttctcc atcgacgtgt gcatgccttc gtgcgcttcg
      721 cactccgacg ccatcagcat cacttcgcgg cagccgtagg tatcgaacgc cgcgccgaac
      781 gccttgtcga tctctttgcg atcgtgcggt agcaggcctt cggcgccggt cagcacgggg
      841 atgtcctgcc agtcgcgcag attgcgatca ttgacgaacc gcgcgagcgc accggcgcca
      901 ctcgcgtagg cgaccatcac ctgcggcttg aagcggcgga tctggttgac cacgttcatc
      961 agcgcttctt cgctgcgcgg cgtgcagtcg agatagagct ctcgcttgta cgcgcggtcg
     1021 agcgtcatct tctgacgctt ccaccacgtc gacgacgaca tcggcacgaa gccccagtag
     1081 tgcatcgacc gcttggcgat gtcgtagccg ccccagccgt agccacggag gcggatcgca
     1141 tcgcgccagt gccgcgactc gtggttgtag agaacttcga ccggctggcc ggagctgccg
     1201 ctcgtgacct tgcggatcac ggcgacgggg ggcgcgtcgg cggtgcgctc tttcatcgtc
     1261 gcgcgcagcg tcgggcgatc gagcagtggc agcttgacga gatccgccgg tgacgtgaag
     1321 tcctcgggca cgaggccgcg ctcgtcgagg agctggcggt agtacgccgt gtgcttgtac
     1381 gcgtggcgca gcaggcgccg cagaaatccg gtctggagct cgcgcagctc gggcgccgac
     1441 cacgtcgatg tctcttcgag atacttgagc agtggcacgg tcggccggcc gcgcgccgct
     1501 tcgaatacgg ggaacagcgc cttcgagaga atctggccgt agatgtccgt cacggcgatg
     1561 gccgctccac cacgacgacc ttgcgcttgc ctgcggcggt cagcgggatg tcctcgacgt
     1621 actcgatgtt gaacggcgcg cccggcaggt acttatcggc gaacgcgtgg atcgcgcggt
     1681 agttcgcgtc cggcagcttc ggtcccgccg tcgggacgac cttcatgatc acgctgccgt
     1741 cgatcttctg gacgacctga aacttcgacg cgacctgatc catcacgccg aacaagatgt
     1801 tgaacacgag accgccgacg gcgttgccgc gtccgtcgcg cagcgtctcg gtggtgcggc
     1861 cttcgatcgg gccgatcttc gtcaggcttc gcccgcacgc gcagaccgtc ggctcgtgcg
     1921 cgactgcgag gtcgccattg acgtagcgga tcatcgggca cgcaaggttg tgcagatcgg
     1981 tgatcagcac ctcgccggtc tctccaggct tcgcggcgcg cgtcgtgccg tccggattcc
     2041 ggacaacgac ctcgacgatc atcgtctcca tcgaggtgtg catgcccgcg tgcacttcgc
     2101 actccgacgc catcagcatc acttcgcggc agccgtacgt ctcgaaggcg tcgccgaatg
     2161 cctcttcgat cgcctcgcgg tcgtgcggcc acagccgctc tgcaccgacg agcaccggga
     2221 atgacttcca cgtgcgcagc ttgttctcgt tgacgaactt cgcgagcgcg gcggcgccgg
     2281 cggcgtacgc caccatcacc tgcggcttga aatcgcgaat ctgctcgacg gcagcggtca
     2341 gcgcttcttc gccgcgcggc gtgcagtcga cgaacaggtc gcgcttgacc cagcgatcga
     2401 tctcgatctt gcggcgatgc gtccacgtct tcgcaggcgg gccgaagccc cagtagtgca
     2461 tcgcgcgaac gccgaggcga tagccgcccc agccgtagcc gcgaaggcgc gtcgcatcac
     2521 gccagtgccg cgactcgtgg ttgtagcgaa cgaccacggg ctcaccggtc gtaccgctcg
     2581 tcgacttcgt gatgacccac ttcggtggcg ctcccgcgag gcgcgtatcg atcgtcttgc
     2641 gcaagaggtc gcggtcgagc agcgggaact tgtgcagatc ctcgacgctc tcgatgtcct
     2701 ccggccgaat gccgcgatcg tccatcacct cgcggtagta cgcggtgtgt tgatacgcat
     2761 gacgcaacag gcgccgcagg aagccgacct gcagatcgcg aagtgcatcg agcgaccaac
     2821 gctccgtttg ctggaggtat ccgagcagcg gaacagttgg cctgcctcgt gctgcttcga
     2881 acgccggaaa taaaactttc gagagcagcg gaccgtagag atccataacc gtcagtcgtt
     2941 ctaacatcca ccacacgtgc tgtcccgtct ccgcaaaaag aacctccacc agtggctgcc
     3001 cgattacgca cgtcacctcg tgcggcgtgc gcgcacgcca cagtcgggaa accatcgtca
     3061 cctcctgttc gcactctgcg atcactacga accacattgg ggcaaggcac ccgacgacgt
     3121 cgcgaaggcc cgtgtcgacg cgtgggccga caactacccc aagcttggtg agttcagaga
     3181 tagtgacggc agagcgcctc gccatggctt cttctttccg ggcgaggagt acaagccgta
     3241 ctacctcgac aggctcggcg agctcgcgcg cgctggcttc ggcgaagtcg agttccatct
     3301 tcatcacgat ggcgacaccg cggagacact cgctccgcgc attcgcgagc acatcaagac
     3361 gttttcagag cacggccaca tctctcgaga caaagccggc aacttccagt gggcgttcat
     3421 ccacggcaac tggtgcctcg cgaacggccg cccggacgga cgctggtgcg gcgtcgacga
     3481 cgagctgcct ctgctcaaga agctcggctg ctacgtcgac ctgacgttcc ccgccgcgcc
     3541 cgatccgtcg cagcccgaca aggtcaatca gatctactgg cccgtcggcg atctcaacaa
     3601 gcgccgcagc tacgaccacg gtgaacgtgc acgcgtcggt catagctacg acgatcgtct
     3661 cctgatgatc acgggcccgc tcgcattcgc gcgcaaaggc accgtcggga ttcgcatgga
     3721 gaacggcgcg ctcaccggcg acgatccgcc gaacgcgcag cgcgttgcca cgtgggtcaa
     3781 ccagggcatc cacatcgaag gccgtcccga gtgggtattc gtcaaagtgc acacacacgg
     3841 cgcgatcgag aagaccgccg cttcgctcct cggtgatgga gcgcacgcga tgcacacggc
     3901 tcttcagcag tacaagcgca acggctggca gctgcactac gtcaccgccc gcgagatgtt
     3961 caacgtcgcg agggctgcga tggatggcaa gaacggcagc cccgccgact acttcgacta
     4021 cctcgtcaag ccgccgcctg ttgcgcaatg atcgtgcagc gcgcggcggc ccgcggcgct
     4081 gtagaatcga cccatgcgca ggagcgtggg acgcgcgggt ttgattgcga cgagcgtgat
     4141 cgccgcggtg cagctccagc aggcatgcaa gttcgaccac ggcgcgctcg tcacggggat
     4201 gcccgacggt ggccccgacg cgctgcccga tgcgagcgaa tgcaccgccg cgatggacat
     4261 gtgcctcggc gatacgctgc gccactgcga cggtcctggc gcgatggcag tcgatcgccc
     4321 ctgcggctgg ggctgcatga ccaaccgttg cctcgaggtc gttccggcgg gaagcggcgg
     4381 caacgcgagc gatggcgtga tgccgagcga cgtcgttgcg gtggacatga cgaccgctgt
     4441 cgagctcgtc gaaggcctga tcgtcgacgg cgacaacggc cgcatcggga ccacgatgaa
     4501 tcccacgtcg ttgcgtggct cgggctcggg cgtgatcgcc ggcatcgatt ggcagatgcg
     4561 cggtcccgtc tcgatgtttc gcttcaagag cctcaagatc acgacgagca gcgcgggctc
     4621 cgtggccctc atcggctcgc atccgatcgc cttcgttgcg gacgaaggag tcgaagtcga
     4681 cggcatcgtc gatgcgcgcg gcacgtgcgc gggctcggcg gcgggtcccg gaggattccg
     4741 cggcggcgcg atcgacacgt cggcgacggg cagcggcgca ggcaaggcgg tcaatgcatc
     4801 gagtgtcggc ggtggcggcg gcggtcacgg cgacgatggc ggtgatggcg gcaactccgg
     4861 cgggcaaggt ggccagagct tcggcggccc tgcgatcgcg ttgttgctcg gcggcggcgg
     4921 tggcggtgga ggtcacggcg gcggcggcgg gaccggtccc ggcggtggtg gcggcggcgc
     4981 gctgcagatc gtttcgaatg gtctcgtttc gatcctcagc ggcgggatca acgcgggcgg
     5041 ctgcggtggt cgcacgggca ccggtggcag cgacggcggc ggtggtggtg gcgccggcgg
     5101 cacgatcttg atcgaagcgc cgaaggtgaa tctcgagggc ggtgtccttg cagtcaatgg
     5161 cggcggcgga ggtggcggtg caggcacgac ggccacgacc ggaacgtcgg gtcagctctc
     5221 cgaagccgca gcccctggcg cgacgaacgg tgcggatggc ggcgcaggcg gcgcagccga
     5281 caagctcgac ggaacgagcg gcgaaacgtc cggtggcgac ggtggcggtg gcggcggtgg
     5341 cgtcggccgc attcgcatca acacaagaga caacatgggc atcctgccgg ccatcatcgg
     5401 cacgatcacg agccctcggc tcagcgacgc gacgacgacg acgacgcgcg gtagcgcagc
     5461 gacgcgttag tctgcttgac gcgcaggcac ggacgacgcg gagccgcccg actgcacgcg
     5521 cgcgcgctcg ggcgcttcgg tctgatcgcg cacgtggcgc ggaacgcctt cgacgtccca
     5581 cacaatgcgc agcgcctgca gcgccttgag cgtgtaccag ctgatatcga tctcgtacca
     5641 gtagaagcct tggcgcgccg accgctgata gtggtgatgg ttgttgtgcc agccctcgcc
     5701 gaacgtgagc agcgcgagcg cgaggttgtt gcgcgagtca tccgtcgtct cgtagcgacg
     5761 cgagccgagg acgtgcgcga gcgagttgat gcagaacgtg aggtgccacg agaagatcag
     5821 cgacacgaag aagccccaca cgagagcggt ggtgccgccg atcgcgaacg tgatgaccgc
     5881 ggtgccgatg acgcaaatga gatcgtggcg gttcagccac cgcagctcgg gatacttcgc
     5941 gaagtccttg atgcgatcgt agtccgtcgc cttgaagcgc ttcgcgagga tccactgcat
     6001 gtgcgaccag atgaagccgc gctgccgagg cgagtgaatg tcctcgggca tgtccgagta
     6061 cttgtggtga cgacgatgat gcgcggccca ccagagtgga ccttgctgcg ccgtcgtcat
     6121 gccgagcaac gcgaggatga actggaagac gcggctcgtc ttgaacgtgc gatgggagaa
     6181 gtagcgatga aagcctgcgg tgatcgcgaa catgcgaacg gcgtacgtcc cgatcagcca
     6241 ccaaaacgcc gtccagctcc aacccacgat gatcgcgcca acgagagccg cgacgtgaac
     6301 accccagaac ggcaggtttt cgcgccagcg cggcttatct gcctgcgcgc cggtgaactc
     6361 gacggattcc atggccaaag catcccccat cggcgcggga gtcccggtca cggtacctgc
     6421 agggttacgg cacgtttagg gccttctatg acacgaagat gacgagcaat agtttgcaac
     6481 gctgtcacgt cttcttggtg ggaggcgccc gatgatgagc gggtgacgcc gttctgcttc
     6541 gagcacgtgt tcgcagcacc ctctcctgcc gcggtgctcg cggcctactt cgatgcgagc
     6601 caccagctcg agcaggaccg cgcgatcgac attgtcgagc gcgagatcat cgagctcgtc
     6661 gacgacggag acacgatccg ccgcaccagt cgcgtcgtgc cgcgtcgcca gttgcccggc
     6721 tttctccgca cgctgtcgtc gggtccgctg cactacctcg aggtcgccac gtggcaccgc
     6781 gccgccaacc ggatctcgat cgaaatcaga ccttctctca tgggaggtcg agcccagatc
     6841 tgcgccgagt atcagctcga cgtggtggcg ccgaacacca tccgtcgtcg ctacgcgggg
     6901 catgtgtcgg tggatgtcgc cctgctacga gcccgtgtcg agcgaggtat cgtcgccgaa
     6961 ttcgagaggt cgatgccgct tgccgcatcc tgcacccagg cctggctaga tcggcagtcc
     7021 cccacgtctg tgtcagctcg cacttaaatc cgggatattc ctcaggcata atgttctcgt
     7081 gacgattcct gcggggacgc tggtccctcg cgacgctcga gatttcggca gctaccagct
     7141 cctcgcgaag atcgcgacag gcggcatggc cgagatctat ctcgcgcgtc cagcagcgtc
     7201 gcagagcacc aaagaattga tggtgctcaa acgaatcctt ccgcatctcg ccgaggatga
     7261 gcacttcgtc acgatgttcc gcgacgaggc cgacctcgcc gccaagctcg tccacaagaa
     7321 cgtctgcaac gttctctcgt tcggtcaatt cgcgggcacg tggttcatcg ccatggagta
     7381 cctccacggc gtcccgctct cgcgcatgat gacgaagctc tcgaaggcgc agaagatgct
     7441 cgatttccgc atggtcgcgg gaatcatctg ccaagcctgc gagggcctgc acagcgcgca
     7501 cgaagcgcgc gacgaaaagg gccagctcct cggcgtcgtc caccgcgacg tctcgccgcc
     7561 gaacatcatg gtcaccgccg acggcacggt gaagctcctc gacttcggca tcgcgaaagc
     7621 acgcggcgcc aactcgcgga cgcgcacggg caccgtcaaa ggcaagaacg cctatatgtc
     7681 gcccgagcag atcctcggca agccgctcga tcggcgcagc gacatcttcg cgctcggcgt
     7741 cgtcatgtac gagatgcttg cgatcaagcg cctctttcat cgcgactccg acttcctcac
     7801 gttcaaagcg atcaccgaag aagccatccc cgacatccgc gatcgccgcc ccgacctccc
     7861 accgggcatg cgcgccgcgc tcctccaagc aatggcccgc gatccagccg gccgcttcga
     7921 caccgcgcaa ggcttcggca acgcgatcaa gaactcggtc gcgacgctcg gcggacccgc
     7981 ctcgcccgcc gacctcgctc gcctcctgtt caccgacttc tccgacgaga tgtcgtcgcg
     8041 cgacgagatc ctcaaagcgg ccgacgatcc caacgcgtcg ccgatctcga tctcgacgaa
     8101 gcccgccgtc ccgccgccga tcccaaagcg cgaagtccag ctcgcaacga gcggcgagat
     8161 gcaccaagcc acgcccgcca cgggacgctc gcgcacgccg accggcaacc tcgagcccgg
     8221 cgtcatcatc gcgagctcgc acggcgcgca gaccgccaag atcacgatgc ccgatctcgc
     8281 gaacgtcctc gatctctccg aagacgtcgg cgccgaccag tggatggcgg aaggcggcac
     8341 agacctcctc tcgtcgcatc gctggcgctc gctgcgcaac ctcgcgctcc tcctcatcgg
     8401 catcgccgtc gtcggcgtcg gcatctacat gttcttcaag acgacgtccg acggcagcga
     8461 tacgaaagtc gtcaccgcgc cgccgatcga tgccgcagtc gtgcggaaga acgacccgcc
     8521 gccgctcgac gcgcccgagg tcaacaagaa cgacatcatc gcgctgtcga agttcggctt
     8581 cttcacgatc gacgcgtccg ccaagacaac gatctacgtc gacaacgttc gctacggcga
     8641 aacgccgatg cagcgccttc cgctcacgcc cggcccgcac aaagtaaaag gggtcacgaa
     8701 gggcaagaag ccgcgcgagt tcatgatcac gatcatcggc ggacgcgaca ccgagctcga
     8761 gacgatcgac tgggaagggc ccgcgcctcc gccactgccg aagtgatccg cgcagaacgc
     8821 gatcagctga gcggcgctgg gcggatgctc gagatttgca gcgatgattc gggcgacgtc
     8881 acgatctgca cgcgatcacc tgcgtgaaaa cgcggcacca gctcggcggg accttggaag
     8941 tgcaggtcgg cgaggttgtg aacgtcgagc tcttcgcctt cttcgtcgat cgcgcgctcg
     9001 atatcgagct ggatcttggc gacctggcga gacgtgacgt gctcgaagcg agcgaccagc
     9061 gtgtgcagct cgatggtcag gattctccca accacgcgca gacccgcatc ggacgccata
     9121 tttctatggt acgcgccgag cgcgcgctgt ctacgagcgt gggccggaga cttcgatgcc
     9181 gtcagttgcg cgagctacgt agatcacttc ttcgtcgccg gcggtgaggt cgtgctgtgt
     9241 gctcgcttgt tgcaccagct catcacctgc ttgcagcact tttcccgtgg agtcgcgcaa
     9301 cgtacctgtg aggatcacgt tgatctcttc gccgcggtgc gtgtgccacg ggaacgtaca
     9361 tcccggttga atgcggacaa agccgcagtc ggcggtggca caggccggac caccgtcgaa
     9421 gtggatcagc gaaatgccgg gcatcggggt ttcccatgac gccgcgcgct cgacgagacc
     9481 gagcagctcg cgtgcgcgat cgacggtgac gtcgaagatt tttgccatgc gatcggcgaa
     9541 gcgatcgaag cgaccgccac ccgcggaggc gaggaggcga gccttgacgt ccggcgatgg
     9601 ctcgacgggc gcgatcagcg cgaggtagga gtcgagctcc gcggcgagcg cggggtcggc
     9661 ttgtacggcg cgttcgacat cggcggcctc gtcggcggcc agcgcgttga tcgcgtagag
     9721 cgggaggagc tcgtgaatgt tcgccggggc cgtcaccggg tgcctcccag agtatcgcga
     9781 agtttttgca gacccgccgc gagccgcgac ttcaccgtgc cgatcgggat cgcgatgcgc
     9841 tcggcgatct ccgagcacga gaggccttcg aagtaggcca gctcgacgac ccgcttttgg
     9901 tcgccgctca gtccgtcgag ggccgtgcgc acacgtgcgt ggtcggggct cgaacgctcg
     9961 gaattatcga cgacgacctc gagcccggca tcacccgcat tgcgggagac tcgggcggac
    10021 ttctggaggt cgagggctcg cgagcgcata cggatagcca accaggtacg aacccgacct
    10081 cgctttggat cgaagtcttt agctgcgcgc caggcctcga ggaggacgtc gtgtaggagg
    10141 tcttccgcct ctcggcgttc cctcacgatt ttcagggcga tggccaggag aactcccgag
    10201 tggcgctcgt agatggcggc cagggcgctt ctgtccccct tcgccatcgc ggcgacgaga
    10261 gctgcatcct cggcgtcatc gcctgggtcc atgtgggccg accgtaagct ataacgcgcg
    10321 cggtgtcact tcgtgaagcc gtacgcgccg aagcgaccga tcgcacgtgg tcgcagggag
    10381 tcacactcgc ccgggaaggt cgcgtggtcg gcatcgggcg cagcgacgag gaggtcgagc
    10441 tcgaggttcg cgtgcccggt cggccgacgc ccttcgaggt cgtcctcgat ctagagaacg
    10501 acgaatggca gtgcaactgt ccgagcaaag aagccgtgtg ctcgcacgtc gtggccgcgg
    10561 tgctggcgaa cgagcaagcg ggtgatgcgc tgccgacgtc gtcgaaggtc ggcgcggcga
    10621 ttcgctactt gctcgagccc gatccgggcg gcgtcaaagt cgagcgcatg ctcgtgcgtg
    10681 acggcaacag cgagccgctc gtcggatcgc tcatgtcgct cgtcggcaag ggcaaggcga
    10741 gccacatcgc gacgcaggag accgacttgc tcgcggacca attgctcggc gtgcgcagcg
    10801 tcgtgagtgg cgagaagctc gaccgcttgc tcgaggcact cgccgacgca cgcgacgtac
    10861 gctggcgcgg agatccggtc acgacaaatg gagagccggt gctgccgcgc gcgatcgtcg
    10921 aggatttcgc ggacggagtg cgtgtccggc tcgagcgcga tccagtcgtg cgagaggttg
    10981 tcgccattgg gctcgcgcgc accgaggaca acgtgctgcg gccgatcggt gcggtcgatc
    11041 tcggcggaac gcgcttcgag aaactaccgc agtcgttcga cgtgccccgc tcggcgattc
    11101 cggagctgac cggcaagacg ctgcccgcgc tgtcgcagcg gatctccgtc gtcgtgaaga
    11161 gctctacgct gccacaggtc ggcaagcgcg aagaaccgcg catgcaaatc gacgtcgagc
    11221 aggatggcga caagctcatc gtgatgccga cgctcgtcta cggcgatccg ccgcgtgcgc
    11281 gcatcgacgg tggccgcacg ctcgtccatc tcggcggcac gctgccgatt cgcgacgaag
    11341 atgccgagcg ccgcctgctc caccggttgc gcgacgagct caacatggtg cccggtcgcc
    11401 gcgtcgagct cacgggccgc gatgcattcg cgatgcagca acacctcggc aactggctcc
    11461 gcagcgatgc gaagggcgcc gcgacgtcga aagctgtcca gctcgagctg cgcatcgcaa
    11521 tcgacggcga tcgcctcgat gtcgatctag aaggcagcgg cggtaagagc gcttccgtcg
    11581 gcgccgcctt gcgcgcgtgg cagaacggcg tcgatgtcgt gccgctctcc ggcggtggtt
    11641 ggggccgcgt cccgatggat tggttcgaca agcacggcga acgcctcgcc gatctgctcg
    11701 cgacgcgcac cagcgatcgc cgcgttccga tctattcgct gccggatctc gcacggctct
    11761 gcgaggacct cgatcagccg ccgccgcccg agctcgagcg actcaagccg ctactcgaga
    11821 acttcgacgg ccttcgcaag gcgaagctcc ccaaaggctt ccaaggcgag ctacgcccct
    11881 atcagcagag cggtctcgac tggcttgcgt tctgccgcga agccggcctc ggctgcgtgc
    11941 tcgccgatga catgggcctt ggcaaaacga tccaggcgct cgccgcgatc aacggcaaga
    12001 cattggtcgt cacgccgaag tccgtcctct tcaattggct cgccgaagcg gagaagttcc
    12061 ggcccgatct caaagtcacg acctacgcgg gcacgcggcg cacgctcgac acgtccgccg
    12121 acatcgtgct cacgagctat ccgatcctgc gcaacgacat cgacgacctc gccgagacga
    12181 cgtgggacac gatcattctc gacgagtcac agacgatcaa gaaccccgac tcgcaggtcg
    12241 cgcgcgccgc gtatcgcctc aagggcaact ggcgcatcac gctctccggt acgcccgtcg
    12301 agaatcgcct cgacgagctg tggagccagc tccacttcac gaaccccggc ctcctcggcg
    12361 gccgcgccga tttccaagag cgctgggccg agccgatcaa cctcggcgac aacgccgccg
    12421 ccgcgcgcct ccgcgaccgc attcgtccgt tcgtgcttcg ccgcatgaag agcgaagtcg
    12481 ccaaggagct gccgccgcgc accgactcga tcatgtacgt cgagctcgac gagaacgagc
    12541 gcaccaccta cgacgcgatc cgcgccgcga cccaacgcga gatcgtcaag ctgctcgaat
    12601 ccggcggcgg catcatggcg gcactcgaag cgctgctccg cctgcgccaa gcgtcctgcc
    12661 acctcgcgct cctcccaaat ggcatgcgca cgggcgagac accgcaatcg tcgtcgaagc
    12721 tcgaacgcct catggaagcg ctggccgacg cagtcgccga cggtcaccgc gcgctcgtgt
    12781 tctcgcagtg gacgtcgctg ctcgatctca tcgagcctca cctgcgcgcc gccgacacga
    12841 agttcgtgcg cctcgacggc tcgacggcgg accgcggcgc tgtcgtcaac gagttccaag
    12901 ccgacgacgg cccacctgtc atgctgctct cgctcaaagc aggcggcacc ggtctcaacc
    12961 tcaccgcggc cgatcacgtc ttcctcgtcg atccgtggtg gaaccccgcc gtcgaagatc
    13021 aagcggccga ccgaacgcac cgcatcggcc aagacaagcc cgtgatggtc taccgcatgg
    13081 tcgcgcgcga cacggtcgag gagcgcatcc tcgagctgca agagcgcaag cgtcgcctcg
    13141 ccaatgccgc actctccgaa gccggcggcg cgacgtcgat cacgcgcgaa gacttgctcg
    13201 cgctgctgtc ttagttcgcg acgagcgggc cgttgatgcc gatgtcgtgg tgcttcacga
    13261 tctgctgcgg cgacagcgcg taatcgtaga tcgcgagctc gtccatgtcg ccttgcagaa
    13321 acgtgttgcc cgccggatac gcgccgatgc tgctctccat ggggacggcc ggcatcgtgt
    13381 caggagccgt cttcgcgggg ccgacctgaa cgccatcgaa gtagaggcgc acgaccgcgc
    13441 cgtcatacgt gccgacgacg tgaatccact ggttcgctgc aatcggcgtc gggttcgtga
    13501 cctgcccgcc cgtattgacg atgcgctcga gataaacgcc gttcgagttg atcagtaacg
    13561 cgtagccgtc gagtgggccg ttgcccatcc gcgtctcctt gatgaaatag atctggtagc
    13621 tgtcgacgcg ctcgtcgcga atccacgcct ctaccgtgaa cggcgcccgg ttcgggaatt
    13681 cgaggccgct cgggatcacg gcgaagcacg atgagccgtt gaaatgcagc gcggtgtcgg
    13741 cgtcggcgtt caacgggcct tgcaccgcgg cgcaggtgcc cttgaacgtc gcatcgaatt
    13801 gccccatctc atcgcgtccg ggtgccggcg tgccgagccg ccagtacgcc gtcgggtggt
    13861 cgtcgagcac ggcgtcgcga tatgtagtca ccgctcgcgc gtcgggcgtc gatccggcgt
    13921 cgaagccgag gcggccgcaa cccgcgacga tgccaactgc gacgagcgcc ccacgcaccc
    13981 gtccattggc tcacgtcagt ggcgcgagac gcaagatctt gccgtgcacg tcgcccgtat
    14041 cggcatcgat cgcgggatcg atgaagatgc gagtcaccgc gctgttgcgc agaccggcga
    14101 gcatctcgtc gctcatgaag gcgccggcgt cgcagccgac cgcgaacagc tcctcgccgg
    14161 tgcccatcgc cttggcgaat gactcggccg actggccgtc ggagaacaga aagccgtacg
    14221 gacgccggca ccacatgtcc tggccgttcg aggtcaggta ccagatgcgc gcaggcagga
    14281 gctcgacgag ctgctgcatc gaaacgtcct cggccatggc cgggtgtata caacgcagca
    14341 gcatgtccgc accaaccggt gtgccaaggg aagcaaaacg ggttcagccg ccgcatgccg
    14401 actatgcgtt ctgggtgatc ggcgcgaagc ggggcgttct gcgcgacgcg tatcacacgt
    14461 tcttgcgagt gcgctggtcg atcgcgctcg cgctgatcgc gctcggcctc gtgctcgcga
    14521 acgtgttctt cgcggccatc tattacgtga tcggcggcgt gaagagcgcg gacgactcct
    14581 tcttcgagat gttctcgttc agcgtgcaga cgatgtcgac gatcggctac ggcaccgagt
    14641 acccgctctc gacagccgcg acgtccgtca tgatctccga agcgatctcc ggaatcatcc
    14701 tgaccgcgct cgcgaccggt ctcgtgttcg cgaagtttgc gcgcgcgacc ggtcgcatcg
    14761 cgttcacgaa gcacgcggtg gttacgatgc acgacggaaa gccgacgctc atgttccgct
    14821 gcggtaacga acgctcgaac gtgatcgtcg aagcgcagct gcacgtcacg gcggcgatgg
    14881 tcacgatgac cgccgagggt cgcccgttct acaagatcca tgacctcgag ctggtgcgca
    14941 gccgcatgtc aggcctccgc cgtggctgga ccgtcatgca catcatcgac gagaccagtc
    15001 cgtttcacgg caagacggcg gcagacctcg cgaaggccga gtgcgagatc gaaatctccc
    15061 tcaccggcat cgacgacgtc acgatgtcga ccatccacac gctgcacgtg tactccgaca
    15121 aggcgatcaa gttcggtcat cgcttcgcgg acacgatcaa gccgcttgct aacggtgatc
    15181 tgttcctcga cttgacccag ttcgatgtcc tgctccccga cgagcatgct agcgtccccg
    15241 catgattcgc ctggctgcgc tcgtcctgtt cctcgtcgcc tgttccggtt cgccgggtgg
    15301 cccgaacatg aacaaccgca tggcggggcc ggaccccgtc gcgcagagct cggacgtcat
    15361 ctcggccgac atcctcgcgc gcgagccggt agcgaataac gcgaaggtca agcacgtcct
    15421 gatcggttgg gaaggcatgg acggcagcga cgagcgcgcg aagaagcgca cgaaggccga
    15481 tgccgaagcg ctcgtgaatt cgctgatgac gcagatcaag gccggcgcca acttcgagat
    15541 gctgatgaag cagcactctg aagatccggg cagcatcggc ggcaattcgt acgacgtctc
    15601 tccggatgcg ggcctcgtga tcgagttcaa gcagctgtcg ctgcggctca acgtcaacga
    15661 ggtcggcgtc gtacagacga actacggctt ccacattatc aagcgcgtcg aatgacgagc
    15721 ctggcgatca ttctcggttt gctcgtcggc gcgcgccacg cgttcgaacc ggatcacctc
    15781 gccgcgatct cgacgctcgt cacgagcccg cgatcgccgc gcagcgcctc tatctcgacg
    15841 cgctcagggc tgttcctcgg cctgctctgg ggcctcggcc acaccatcgc gctcctcggc
    15901 gtaggtgtcg cgctcgtgct cgccggcggc ctgcttccca cgcgcatcgg caacgcgttc
    15961 gagctcgccg tcgcaggcat gctcgtgatc ctcggcgcgc gcgcgatcgt gcgcggtgtt
    16021 cgcaacaagg agggccgcac cggcgttcac catcacggcg gcgtcgctca cgcgcacgca
    16081 ggcgcacacg atcacgtcca cctcggcgat cgcgcatatg cgtggcgtcc gctcacgatc
    16141 ggtatcgtcc acggcctcgc aggcagcggc gctctaaccg ccctcgcgtt cgccgagctc
    16201 ccgacgacgt cggctcgcat catttacatg ctgctgttcg gcatcggctc ggtcgccggc
    16261 atgacgctcg cgaccggagt taccggcacg gtgctcgcgc gcctctcgcg cggagacaac
    16321 gcgcaacgcg tgctcgcgat cgccacgggc agcgtgtcgt gtatctgcgg cgtcctgtgg
    16381 gcgctgccgc tgctcgcgta gttacggcga gtcgacgccg gcgtccgcgc cgaagttgtg
    16441 cgggcggtcc ttctcgcacg cgagccacag cgcgaggttg cggcgctcct cttccgttgg
    16501 ctgcgcgcaa tttccgtcga gcttgccgcc gatcacgctc gggcactccg cgggaggcat
    16561 gaagccattc gacgcgttgg gcccgatgcc cgcctgctga tcgatgtggc cttggacctg
    16621 gagaatgccg agcagcgagt cgaagtcgtg aaacagcggc gcgccgttgc gctgcgacct
    16681 gggcagcgtg ctctcgtggc accagttgca gtacttctcc atgaacggct tgccgaagtt
    16741 gtcgtacgtg agcgtcatcg gatcgggcga gggacagacc gttcccgtcg acgttggatg
    16801 gtcgccgcat gcggcaagtg agagcgctag cagtaggtac ttcatctaaa agctcagcgc
    16861 ggtcgagacc acgcccctca ctttgaatgg atcaccgacc aagcccgcct gcacttctgc
    16921 cgttgtatcg acgcggccac tgtgccaacc gatgcgcaag gcaccgacga cgcgtgtgct
    16981 gcccgcatcg ccgacgggaa tcccacccga gacgcggcct ccaactcgca cgtcgtttgt
    17041 cgcgcgcaga tcgccgcccg cgctccacga tagctccgac atcagcatcg gctcgacgag
    17101 cggccacggt ccatgatcgt gatcggtatg gccaccgatc gcgcggctgt aacccgcggt
    17161 tgcaacgacg tcgaagcggt cgacctgata cgcgccgaac aacgcaggca tgagcatcac
    17221 gtggcccatg ctcgtgccgt gagcgcccgc gccgatcggc gtcatgacag cagcggtcac
    17281 accggcgctt gcgtgcgaat caccgacgag cgtggcctgg ccgttcacca tgagatcacc
    17341 gacgtcgtag tagtccgcgc cgtttttctc gaggcgatag aagcctgcag tcacgcccgc
    17401 cccaaaccgc ggacgcgacc accgcaccga cggcagcacg ccctcgtagt tgccgacgta
    17461 gtccatcgtc tcgaacgacg ccgcgacaaa ggcgacgcca gccccgaaca cgtcgcgcgg
    17521 ctcctcggaa tggctttcca tcccgtggtg atgctgcgca aatgcgggcg cgctcgccaa
    17581 ggcgaggaag acgacgatcc ggaggaccat gtacatcgta cgaccgagag ctagtgctgg
    17641 ctctggtacg tcgtacatcc cgccgaaatc tcggcggtgg tggcgctctg tccgagctga
    17701 gccgtcacgt aagtcgtgca ggccgctgaa tccccgccac acagcgtgtt cttcgcggcg
    17761 ccgccgatct cgtgctcgat gcaacacacg acgatcgcct gatcgaccgg cagcgactcg
    17821 acgttgtggt gatcatcgaa gcacgccttg tacgtgtcgt aggactcctc ttcgtctcca
    17881 ccacacgcag caaacaaaaa cgcagtcgaa agaatagcca gtgcaaaacg cataacgcgc
    17941 tcctctagaa aatcgttgaa cgtacaaacg ctggatactg cgcgttcgtc gctacgatgg
    18001 agcgtcgggt ctgcgtggag cgggagctgg atgcggatcg gggagcggtg cgttgatcgc
    18061 gatgagcgcg cgcgacggcg cgacgacatc cgtgacctcg ggtggcacaa acgagggaag
    18121 cgtcgtgctc gcgatcgccg gtgccttctg atggcacgtc ttcatcgtcg gctgcgccgg
    18181 atcgtcgtgg ttgcacttac acgccttcgg attcggacag cagcacgcgt ccttctcgac
    18241 gaacgtttgc agctgcgcga caggcacgag caccgcgagc gcgatcgcga caataactgt
    18301 cgtcgcggcc gtcaaaagtg ctcgcaggct cacgacggca tttgtgcatt ccggcgcgcg
    18361 gctgtcaaat cacgcggaat aacgacgttg atgctcgcgt tgcgcgctgg atgttcccgc
    18421 gcacactcct gatcgtgctg tttcttgcgg cgcagacggc gctgctgtgg tgggtcgctg
    18481 cgcgggatcg cacaaccgaa gcccatttcg cctggacgac gacagtggtc gcgccgccag
    18541 ctgcttcacc gcctgcgccg gtcgtgcttg ccgcgacgcc acccgcgaca acgcgaccga
    18601 gctgtccgcc gcctcgcacc gacgctgcga cgcgcaagcg caacctcccg acgggcgtca
    18661 accacgtcac gacctcggtg acgaacgccg gttggctcgc ggcatgggac gaacatcgcg
    18721 tcctcgtctc gacggacggc gggatctcat tcacgcagat gctcgacggt cccggcaccg
    18781 tcaacgacgt gacgttcgat tgcttcggcc atcccatcgt gagccgcggc gacaagctcg
    18841 gcattcgcga cggcacgcgc gaaatgtggc gcacggtctc cggccttcgg ttcgaggact
    18901 acgcgtatcc gctgctgatc ggcggcgggc ccgacatcgt gatcgcaggc tcggaagtga
    18961 acgacgagtc gcacacgtta ctcgctatca gctcggacct cggcgcgacg tggtggttca
    19021 aggacaccgg cgtgtactgg gactccacgc gcgcagtcgg gcgccaagac gcgaacggca
    19081 cgattcacat cgcgttcacg acggccgatt gcatgagcga cccgacgacg tgggtgcgcg
    19141 tgacgcccga tggaacgctc gcgagcgatg acctcggcga catcggccgc atcgggctct
    19201 acggcgacat cgcgatcgcg acgtacggcg atactggcgg cgtcggctgg aaacggttcg
    19261 gcgagaagca gtggcacgaa gtcaaaggcc tcgcggatac cgaccacacg cgcgtcgagc
    19321 tcgtcgatgg cccgctcgcg cggatcgtct cgaacggcgt gatctacacc gtcgatgcca
    19381 gaggcaacgc aaaggcactg cgtccctggc cgtatggaac ggcgagcgtc gatcgcgccg
    19441 gacagctgtg gggcatcgac gaaacgaagg acggcgaaga tgcgtggctc gtcgcggtgc
    19501 ccggtacgaa ggcgccgatt ccgccaccgt ccgagtggaa cggcaacgag tgactacggc
    19561 gcgtcgagca agaggccgcg acgttcgatc gaccgccgca gcgagcgcag cttcagatga
    19621 tcgatcgtga gaatcgctgc cgagacgaga ccgcccgcag cgagcaaccc aaacgatagg
    19681 tgcgtgacgt gaagcgcggc gagcagcacc gacgcccatg cgatgaagtc gcgacgcacg
    19741 atgtacggcg catacgtggc gatgaaatcg agccatggag gaagcggctc tttggtggcg
    19801 atcgcttttg cagccgcggg cgcgagacgc accgtgttcg gcgcactgcc cggcacgacc
    19861 tcgaagcgac cgacgtagtc ctggctgttc gccgagccga ccgcgacgat gatgttgtag
    19921 tagacgcagt agatcgacca cagatacgtg acgacgctga tcgcgatcgc gagcttccac
    19981 ggatcgacgg ggagctcgcc gaagaagctc ggaccgaagt gctcgtggca gtgccagccg
    20041 agcgcgacca tgtaaccgac gctcgagagc tcgtcgacga cggtgtcgat ccacgcgccg
    20101 aaccgcgacg acaggagctt cacgcgtgcg acttcgccgt cgcagcagtc gacgtatgac
    20161 gccgcgagaa cgacgatcgt gccgagcagc accatgttga agctcgcgtg cgcggtgagc
    20221 cagcatccga gcgcgacgag tacggcgacg acgtacgaga tctgattcgg cgtgatcggc
    20281 gtccacacga gcaggcgcgt cagcggaaac gagacaggcc gatacaggta gcgcgtgatc
    20341 gcgttgtcct gcggcttgat gagaattcga tagagcacgc gatgtgcctg acggcgctgt
    20401 tcagcggtcg cgatcggcgc gcgcgcgatc ggtccatgcg gcacgcggcg cggctcgacg
    20461 agacgggcga cgagcgcggt gtcgtcggca agtccagcga tcacgtcgtc ggcaccgcgg
    20521 cccgtcgcga gcagcgcgcc cgcatacgag ccgggcggta gatcctgaac aaccggcgcg
    20581 tcgggcacga cggcgatcgc gacgccatcg cggtcgagcg ttggcaacgc ttcgataagt
    20641 ggcgcgacga gcggcgtgtg aaccagctga tcggcgcgaa tcacgagcag agggcacgag
    20701 cggcctgctc gccagcgcgc gagctcgtcg tcatccacgc gctccacgac gtgaacgcgg
    20761 gttgccccga ccttcgcggc gacgcggctt gcgcgctcgc gtgctgtcag tcccgcgatg
    20821 cgaacgagcg agagctcgtc cgtcgcgagc accaccgcat cgagctcgaa agccgccacg
    20881 tcagaacatg tacatgcggt caaagcgctg cgctagtccc tgcctcgagc ttgccgacgc
    20941 gcgccaggtg acaggaagtt gtcagcatca aggcgtcacg gcggcagacg tggtgaaagt
    21001 tgaacacgtg aatcgttcaa ctcctcgaaa cgagggcgtg tatacgaatt gcatcaggtg
    21061 gcctcaaaat tatgaaggca gttatccttg ccgcgggttg tgcaacgcgg ctgcgccccc
    21121 actctgacga ccggcccaag acgctgttga cggtcggagg agttccgatt ctccgccgca
    21181 cgatcaccag tctgcttcgc tgcggcttcg accaattcgt gatcggcact ggctacctcg
    21241 agcacatggt tcgcgaggca gtggtctcgt ggttcccgac cctcgacgtc acgttcgtga
    21301 cgaaccccga ctaccgcacg acaaacaacg gctactcgct catgctgtgc gcaccgcatg
    21361 tcgagaacga cgcgttcatc ctgctcgacg gcgacgtcgt gttcgatatc cgcgtcgtcg
    21421 agcaactcgt cgagcacggc cccgactgcc tcgcggttcg ctccgttggt ggcatcggcc
    21481 tcgaagaagt caaagtcacc gccgacagca acgaccgcgt cctcgcgatc ggcaagcacg
    21541 tccccgtccg ttcggcgatg ggcgagtcgg tcggcatcga gctgttctcg gcgtcctcgt
    21601 ccaagcgcct gtttgccgcc ctcgacaagc gtgtgaggca gcagggcctc atcaacgagt
    21661 actacgaagc ttccttccag cagattatcg atgagggttc gaccctttac ggcgtcgaca
    21721 tgggatcgct ctatgcgacc gaaatcgata cgatcgacga cctcttggcg gccaatgccc
    21781 gcctcgccgc ccagccggcc ttcgacgtca gcgtcgacgc agtacgtcta gcggtctaaa
    21841 caccccaccc gcggtcagca tttgatcgtt gtggttggcc ggacctttgg gaatgatgcc
    21901 gttccatgag gtgtcggctg ttttgcatcc tcttcgtcgt gggctgcgct tccgcggaaa
    21961 cgggcgagcg tagtagctcg ctgcaaccgg acgccggtcc gggcggtggc ggcggccaaa
    22021 tggacgcgcc cggcactggg tcgggcagcg gcagcggctc cggcagcggc tccggcagcg
    22081 gctcgggctc gaacggctgc acgacgagcg acctgctcgt gaacgggaac ttcgacggcg
    22141 gcgcgaccgg ctggacggct acgcccgtct cgaccgatcc gatcatcggc cccgtgacaa
    22201 cgccggctcc gcagtctggc gcgaacatcg cgtggatggg cggctacgcg aaggcggcac
    22261 cgaacaccga ctcgctctat caagacgtcg cggtgccggc tggcacgacg atgctcgcgt
    22321 tcaccggcta ctactacatc aacaccgaag agctcggcgg catgtacgac acgggcacgg
    22381 tcgcgctgca gacgacgacc ggaacgcaga tcgaagcgat tcaagcgctc agcaacggca
    22441 acacgacgac gacgtggacg gcgatcaatc acccgttcac gaacctcgcg tcgatcaaag
    22501 gtcagaccgt ccgcgtgaag gtcacgacga acagcgactc gaccttccgc acgaacttca
    22561 tgttcgacac gttcgcactc accgcgaccg tctgcccgta gggccatgta ctcccgacga
    22621 ttcgtcgtcg gagaatgttg aacgccgggt gccgccgcgt gcaccatgaa cgtatgcgct
    22681 tcgcggtcct cgtcgtgctt gccgcttgcg gaccgaccga ggtgacgtcg ccgaccgaca
    22741 acctcggtgg cgctggaacc ggtgaatcgt tcgagagcga tccgtatccg gtcaagcgcg
    22801 tgcgcctcga gcagacgacg ggcgacaaca cgctcgtcgc ggggctctcg ctcgcgtgct
    22861 cggatccgaa cggcacgaaa gagaacagct ggtatcggat cttcgcgctg aaggatttcg
    22921 gcgtcgacaa accgttccag gtcaaccgcg tgaacttcgg cgtgcagacc gcgatcggcg
    22981 agcagcgcgt gaaggtctcg atcggcacgt acgcgggcaa tgcgggctcg gtcgagctcg
    23041 atacgtcgaa gatcgacatg ctcggtctca cgacgatcgc ggtgcccgag ggcaagctcg
    23101 agatgatgca agcgagcttc ccgagtatcg ccgtacccgc cgacgcgaac ctcgtcgtcg
    23161 agatcaagac cgaaggccga ggcgacggcg cgttcttcta tctcggcgcg acggagagcc
    23221 ctgaagtgat gcctggctac ctgcgggcgc ccggttgcag cacggacagg ccgctcatga
    23281 cgtcggcgct cggctactcg cagacgcatc ttctgatctc ggtttcgggc gcgtattagt
    23341 tagtatcgcg ctcgatgagg agggccctgc tgctcgtggt tgccggctgc gcgcagggcg
    23401 gtcgcgacgt gtcgatcgac gcgagcacgg gcctcgtcga cgcgccgaag cagcagatgg
    23461 atgcgccggc ggtcgatgcg ccgcccggga cgcagacgcg cgagctgacg cagacgacga
    23521 gtcagacgat tcagccgcag tcgtcgatcg cgtgcgcgaa caacccgccg ggcacgaatt
    23581 cgaacaacta ctaccgcgtg ttcgatctcg ccgcgctcgg catcacgagc ccgttcaagg
    23641 tcaccgaagt cacgttccag atcgagcaca gcgatcagat caacggcacg gcaggctcga
    23701 cggtcgcggt gcgcgtcggc acgtatgcgg gaacaccaag cgatgtcctc gagctgtcga
    23761 tgatgacggt gctcgcgagc aatccaacgg tcgcggtgcc cgaggtcatc tcgaatccag
    23821 gtccaccacc gacgacgccg ggcggcaccg tgcacgcgcc gatcagcgcg acgattcctg
    23881 cgggccaaaa gctgttcatc gaggtcgact cgcccgacgg ccacaatcag tactacttct
    23941 acatgggcgc caataccgcc ggcgagactg caccggggta cgtgctcgcg ccgacgtgcg
    24001 tcgacggaaa tcagatgccg atctcgaaac cgaccaacat cagcaaggtc gcgccgtcgg
    24061 tctatccgaa ggtgaaccta ctgctcagcg tgaccggcca gtaccaaccg tgatgccgac
    24121 gcgcgcgtag ctcgtgcggc gatcctcggc gatcgacgcg agcacgcctt cgacttcggc
    24181 catgcgcgcc gtcacgcgtg cgtgattctg ttccgcgagc tcatcggcga catctccccg
    24241 cggaggcgcc atctgccccg agagaatttg cgcttgctcc atcacgtcga cgatctgcgg
    24301 cgcgccgcgc acgctctcca gcaacgacag cggcacgagc tgcagcaatg gatctggtga
    24361 gcgtcgcaga aacggcacga gacgcgcggc tgttgccgta tcggtgatcc cgtcgggatg
    24421 aaactccgcg atccccacgc cgggaaggcg ggagttgact cgattgcgaa tgacgcgcag
    24481 ctcggccggc ggcgcttcga tctcgggcgc gagcaccatc gcgactcgcg tcttgggacg
    24541 tgcgagcagc gcgcgcgctg cttcggacca cgcatcgatc gtgggtatgc cccacagcac
    24601 ctcgatagcg acttcgtcgc cgagtcgggc cggtttggcc cacgggcaaa gctcgtactt
    24661 ctcgacgacc tctatcaggt aacggtcgag aactcgccgg acctcggcgg ttcgcgcgtc
    24721 cacgcaacca ttctagggtt tgcgtgtcat cttgatagag tcgtgaaggc attcatcacc
    24781 gcaatcttgc tgtgctcgac cgcttccgcg tgggcggacg atgtggcaag cgagcggtgc
    24841 gcgaagggcg tcgagctcgc cgccaagaac gacctgccgc gggcctcgct ctacctcgac
    24901 ggctgcaccg acgaggcgtc gagcgatgcg gcggctcgcg tcgccaagaa gatccgcgcc
    24961 agtgacctcg ccgcgctgtc gatcagcacg acgcccgacg gcctgaccgc cgagaccgac
    25021 gccctgcccg gcgagacgtt cacgacgccg gcgacgatct acgccaagcc cggcacgtac
    25081 tccgtcacgg tccaaggcct cacgcagaaa gtcacgctcg cagcgcgcag ccgcggcacc
    25141 gtcatcatcg atgcgcgcgt gaagacgcag gcgccccgcg acggcaaggc tgacttcagc
    25201 gaagacacgc ccgagcagac cgcgcacaaa ggcccgccgc ccgacgtcaa gcgcggcagc
    25261 atcgtgccga agaaatacct cggcatcccg gacgatccat cggggcccga gatcgacgat
    25321 ccattcgcgt atcgcgacag ccatctcgcg tggcgcttcg gcgttcgtgt cggcggcggc
    25381 ttcatgagcc agggcgacag cggcgtcggc gtgtcggtcg ctgccctcgc gtcgcgccca
    25441 ctggacggcc ccgcgttcct cgcagcgcgc gtcgactaca accaccgcac gctcgacacc
    25501 atcggcgtca atgcgggcgt cagcgtgaag ctcgtcgaga ccgcatcggt cgtgctcctc
    25561 gcgggcggcg cgctgcgcgg cgagatccac atgcaggaca cactcgcgat gcagcccgtc
    25621 gcgcgcgcaa cgctcggcgg tgccgcggac ctcgacctcg ccatcctctc gctgcccgtc
    25681 gcgctcggcc ttcgctacga gcaaggcttc accgagctgc tctccggctc gcgtgatcgc
    25741 gccgtgttgc tcgaagctgg ctacgactgg cgctagacga tcacgagccc gcgcacgcgg
    25801 tcgaacgtgg cgacgcgatc ttcgcgcggc acgcgtgacc agtcgaaggt ctcgatcagc
    25861 tcgcgcggcg tacacgcctt cggcgcatcg atgagattcg cggcgtgcgc gagcagaccg
    25921 cagagctcgt cggcgcgatg gcgcgcacgc agctcgccga atggaatcga gccgtgcagc
    25981 ttcgcgagct tctcgttctt gccatgctcg agcagcaaga aatggtggcg ataacgtggt
    26041 gtcggcacgc cgagaagccg ctgcagcaag acctgcgtcg cggtgctcgg tgcgatgtcg
    26101 tggccgcgca cgacgttcgt gatggcggtg tcggcatcat cgacgacgac cgcgagctga
    26161 tacgcgatca cgccatcgcg acgccggacg atcggatcgc ccatgtcgcg cgccggcgtc
    26221 tgcgagagat gcatgccgcc atcgtcgacg aggtcgacgc gatcgtcggg caggcgcacg
    26281 cgcaggttgt cgctcacgga acgccaccct cccgcgggca gcggcttatc gcgacacgtg
    26341 ttctcgtagg cccagccgcc atcgggcgca cgccgccctc ctgcgcgatc ggcacggctg
    26401 cacgtgcacg gatagaggcg ctcggtgagc ctatcgagcg ctgcctcgtg cgcccgcgtc
    26461 cggccgctct gcacgacgct ctcgtcccag tcgaggccga gccacgcgag cgcgtctgtc
    26521 atctgctcgc tccacgccgc tttgacgcgc gtcgtatcga gatcctcgag acgcagcagc
    26581 gcctgacccg cccgtgcgcg tgcgtcgagc caaacgagca acgccgacag gagcgtgccc
    26641 ggatgggctt ctcccgtcgg cgatggcgcg aagcgcgacc tcacggccgc agcgtaacgc
    26701 gcttacgagc caggacggtg atggtcgtgc gacgccgcca cttgctcagg acggcgcgcg
    26761 agcgatgccc acgtcgggtc gtagtggttc gagccgtgga ggaacgtcca cgacgtatcg
    26821 ttcagctcgg ggcgtgagtc gatgtggatg tactggccct cggggtacca gccgacgccg
    26881 acgtgcgcgt acgtcttcca caggtaatcg cgaatctcgc gcagctgaat gccgcggatg
    26941 cggaagtcga gcgcggtggc gcggcgatgc gggctcgttg cgctctcgtc gcgaccgacg
    27001 cgatacgcgg acacgtactc gatcgtcttg ccggcgtagc gctccgcgat atccgcgatc
    27061 atcgcgagcg tctgctgctt catcgtgtgc tcacgatgcg tcttgcggca gcggaacagc
    27121 ttcttgatct cggtcgaggt ggcttcgtcc gtggagccat cgcgccagat cgcgaccgtg
    27181 cccttttggt ggtcgttctc gtcgtacagg ctcaccggaa tcggtgcgct cacttcgcca
    27241 gcgaacatcg tggcgacagc ctgcgccggg agcggcacag gagaagcggc ggccgtccag
    27301 cccgccatgg cggtcgtagc gatcttgatc ttcaggatga tcggtagcaa ttcagtacct
    27361 ctgtcggaac tcccgaacgt ttaaacggta cgcacgcgat gattggagat caaacgaaat
    27421 cagcgtcaat gaagtgtacg aattacggac aattcgacat ttttttggat gcgatctcac
    27481 cgtcgctgca cggatgacac ttgcttacaa ctgctggtat tttcagtgcc catgcgcttc
    27541 accgaggaac accagcagct tcgccgcacg gtccgcgatt tcgtcgagaa ggagatcaat
    27601 ccacatgtgg ataagtggga gcgcgaaggt gcttttcctg cgcacgaggt gatgaagaaa
    27661 gcaggtcgcc tcggcttgct cggcgtgaac aaacctgaag cgtatggcgg cctcggtctc
    27721 gattactcgt accagatggt cgtcatcgaa gagatgggca catgcgcgag cggcgcggtc
    27781 ccgatggcga tgggcgtgct caccgacatg gcaacacctg cgctcgcgcg ctggggcacc
    27841 cacgagctga agcaagagtt cctcgcgccc gtgatcgccg gcgagcaaat cgcatcgatc
    27901 gcggtgagcg aagtgtcggg cggctcggac gtcgcggcgc tcaagacgaa cgcgcgcaag
    27961 gacggcgacg actacatcat caatggcagc aagatgtgga tcaccaatgc cgcgcaggcg
    28021 gattggttct gcacgctcgt caacacgagc gaaggcgcgc cgcacgccaa caagtcgctc
    28081 gtcatcattc ctgcgaaggc gaagggcgtc gagatcggcg cgaagatcga caagctcgga
    28141 atgcgcgcga gcgacacgtg ccccgtcttc ttcgagaacg tgcgcgtgcc tcagcggaac
    28201 cgcatcggcc aagaaggcac gggcttcatg atgcagatgg tgcagttcca ggaggagcgc
    28261 ctctacggtg cggtctccac gctgcgcggc atggagggcg tcatcgacgc gacgatcgcg
    28321 tactgcaaag agcgcacgac gttcggccag ccgctgatcg acaaccaggt catccacttc
    28381 cgcatggcgg agctgaagac cgaagtcgaa gcgctacgcg cgctctcgta ccgcgccgtc
    28441 gaggactacg tcggtggtgc cgacgtcacg atgctcgcat cgatggcgaa gctcaaagcc
    28501 ggccgcctcg cacgcgaggt cgccgacagc tgtctccaat attggggcgg tatgggcttc
    28561 acgtgggaca acgtcgccgc gcgcgccttt cgcgatacgc gcctgctctc gatcggcggc
    28621 ggtgcggacg agatcatgct cggcatcatc tgcaagctga tgggcatcct gcccggcaag
    28681 aagaagcgct agcgctactc gggaacgacg ccgctgatcg gaacccagat cgtttcgccg
    28741 gagccgccga cctcgagctc gtagtagccc tgcacgagat tgcgtaccgt cgctgattgc
    28801 atcaggccgt tcgggccggg cacgagcacg cgcccacccg gtgcgatctt ctggctgatc
    28861 tcgccggagg cgacaccctt cgcgacgccg acgctcgtcg agccgatcgc gcccatcccg
    28921 ataccgagga tcgagccgcg aggctcggcc tgtaccggct ctggatcggt cttcttctcc
    28981 gacgacgccg tcggaatttt cttcttgttc gaatcgatct cgccgccgac cacaccaccg
    29041 ccgagcgacg cgccgccacc tgcaccgcgc acgatgtcga caccgggttg cgtgggctcg
    29101 tcggtcgact gcacttccat cgagcccgcg cgacgcggcg acggaaccgc aggcaccgag
    29161 ccgggtgcgg gtgtcgtgat cacgcgctgc gggttcggcg tggcttcgag gcccgacgac
    29221 ggcagcgatg gaatcggtgg aatgggcgcg ggcacgggtc ccgccggcgt gatcttcggc
    29281 gcgccactgt gaccttgcat cgtcgacttg ttcttgacct ggatctcgcc ggacgcttcg
    29341 ccgggtcgac cgtctgggct cggctggctc tgcgggcgct tcgccgccgt tggaatcggt
    29401 ggcggcgccg tgttcgtctt cgtgcgcgac tgcgcgatgc cgggcggcgt cgggggcggc
    29461 ggaatgacga gaccggcagg cttgttcgcg cgcaccgtct cgagcaggct cgactctggc
    29521 gcggcgctag ccatcggcgg ctggatcgtc ggcgcttgca cgcggcgctc ggtctcgccg
    29581 ttcgggcctc cgtggcgcgc ttgtggcgtc ggcaccatct cgtgccacgg acgcgagccg
    29641 tcgtcggcga tgatcgacat gtgaccgaag cgcacgagct cgacaccgaa gaccgcgcc
//
LOCUS       DATLBZ010000206        27597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_947672, whole genome shotgun sequence.
ACCESSION   DATLBZ010000206 DATLBZ010000000
VERSION     DATLBZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 27597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27597
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947672"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2054)
                     /locus_tag="VMZ53_10810"
     CDS             complement(<1..2054)
                     /locus_tag="VMZ53_10810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS28997.1"
                     /translation="MPADPLAPFGEAARTWFSQSFAQATPVQDRGWSSIASGKHTLML
                     APTGSGKTLAAFLWCLDRLSREPSEGTRVVYVSPIKALAYDVERNLRAPLAGLQRLGA
                     GAAITVDVRTGDTSQKERERQRKDPGQILITTPESLYLILAGRSRERLRNVDTIIVDE
                     IHAVAPTKRGIHLGLTLERLSYLVTSSGNPEPQRIGLSATQRPLIEVARYLGGDRPVE
                     IVDASARPALDLQIVVPAADMDNPGPAPAKPKPNPNAFQKENAAPATDGGGMWPLIYP
                     KLLSEILAHRSTIVFVNSRRLAERVAQQLTELAVQQGHAEPGVELVRAHHGSVARHQR
                     MEIEEALKAGRLRAITATSSLELGIDMGAVDLVLQVESPGAVSRGLQRVGRAGHHVGG
                     TSIARILPKFRGDLLEATVVAHEMLAGNVEAIRVPDSALDVLAQQIVAMVAADPWQVG
                     ELERIVRRAASYRDLSHAAFVGVLDMLAGRYPSDEFAELRPRLTWDRQADILVGRKGA
                     RLLSVVSGGTIPDRGLYGVHLGERGPKIGELDEEMVNESRKGETFILGATTWRIEDIT
                     RDRVVVSPAPGEPGKMPFWRADGPGRPVELGKALGAFCRELDGKLVADLPAAQSWLQS
                     EYNLDDLAAGNLVRYLSDQRAQAGCVPTDRAITIERFRDELGDIRVCILSPFGSRVHA
                     PWA"
     gene            complement(2082..2513)
                     /locus_tag="VMZ53_10815"
     CDS             complement(2082..2513)
                     /locus_tag="VMZ53_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28998.1"
                     /translation="MFSIAARIRPSLAGALLLTAIACVPWAEHHQQRGIGPLAADIVW
                     ATDTVRAPRADLAFETPPSEGSDSIVLTPWDRVQLAPQHFDVLVPPKHPDAQPWPRGM
                     VIGLNTPSVDPGIQQWFGSPLANVMSAFLAPWAALAHQLGA"
     gene            complement(2588..3259)
                     /locus_tag="VMZ53_10820"
     CDS             complement(2588..3259)
                     /locus_tag="VMZ53_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS28999.1"
                     /translation="MSPRKLAPFFLLVALINAAAVATRFDLVAAKLPAGVPLGVMLAV
                     LPLIFVAGYFEGRLQYESTLDGLPLWMRIKSVPVKLAFTFAFIYLTCVAFQTWKVSIG
                     PLNPTPPASFPAQTRAMWFAMFTAGMFFPFYLAAAGVLIPILRVLTAPLRKLPDLAGG
                     FAAIVVGGGVGVLVMSALQSVAIGSFIKRVQATISANPALSIGITLGTTLGPLLLGAI
                     LDRDD"
     gene            3561..4322
                     /locus_tag="VMZ53_10825"
     CDS             3561..4322
                     /locus_tag="VMZ53_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29000.1"
                     /translation="MVSVALIATILAVVAGGRALHRHERRNPNERMRRLLKSTKRVRI
                     AELPESQVGCTIGRTRPLEMTSLVEAALTGRPCFFYIAELEVRAGSGWQLVARERVGG
                     PFIIQDASGRAVVEPDGARLDIAFDFVDHVWGNPTAREHQFILRAKPDAVGLPFTHQV
                     RFREAVIGADELVSVLGAGVREADPLAPPVASYRGEPVTRLRFSSSPDAELLVSAAKE
                     TIIGRDSIVTSISGGGASEPRVGDGGAGAGTKDDD"
     gene            complement(4319..5884)
                     /gene="dnaK"
                     /locus_tag="VMZ53_10830"
     CDS             complement(4319..5884)
                     /gene="dnaK"
                     /locus_tag="VMZ53_10830"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HUS29001.1"
                     /translation="MGRVIGIDLGTTNSCVAVLEAGEPIVIHNQEGGRTTPSMVSWNA
                     DGEVVVGAASKRQMVTNPQRTVFGVKRLIGRKATDPDVLSLARRLAYKVVAARNGDCW
                     VDIQGTPRSPQEISAHVLAKMKRVAEDYLGQPVTEAVVTVPAYFDDAQRQATKDAGKI
                     AGLTVRQILNEPTAAALAYGLHNKSNQRIAVFDLGGGTFDISVLAIENGVFEVLSTYG
                     DTALGGDDFDRVIIDLLADEFQGMTNIDLRGDSVALQRLKEAAERAKIELSSAMTTDV
                     NLPFIAAGPDGPMHLMRELERGQLERACKPLMERLEAPCTRALADARCTPEDIDQVIL
                     VGGMTRMPAIQEYAERIFGKPGSKGVNPDEIVAMGAAVHSGIMGGELQEVVLLDVTPH
                     NMGVKVAGDKMSVVIPGNTSIPTRAKKVFATTEDNQTFVAIEVYQGENENASKNRRLG
                     RFVLGDLAAAPRGRTKVEVAFTMDADGILEVSAVEVGTGRASTVTIEASSGLTQDEIN
                     QLGNRLATSRARG"
     gene            complement(5911..6957)
                     /locus_tag="VMZ53_10835"
     CDS             complement(5911..6957)
                     /locus_tag="VMZ53_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29002.1"
                     /translation="MQLRISHGPYSALGISEYATTEDIRAAFLSLTKQFHPARFGRMS
                     TDLQRLSNEVFLGIKSAHDQLQRALGGTHTRPGLGSASSSQAIPVMTDGAGTGRTPAP
                     HLLRRTGQVPIDQARTTGQGRAQLGTQPPVNTTQTQQLARGTDRAGVPQRPSTPVIQP
                     QGTRPATPPMNTPAHGMQRPMTPPMNTPAQGMQRPPTPAQGMRPPTPGQRPVTPVAAR
                     PTTPVRPTTPAATRPSTAPMMPPKPSADLNPGTVRGVQPAPAFDERSALREAMMMLNE
                     KRWGDARQALHNLAAKIPQSKSYRALLCLARGREAHAAGRPDEATLEYQRALQLDPNL
                     DLANQALAELGRRR"
     gene            complement(7042..9048)
                     /locus_tag="VMZ53_10840"
     CDS             complement(7042..9048)
                     /locus_tag="VMZ53_10840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29003.1"
                     /translation="MADSSPLAHSPTLSAAEPHGGTEQLVAGTKVGDYVVDRFIGSGA
                     MGEVYAGKHPVIGKRVAIKVLRHELAASAEAAERFIREARAVNQVDHDNVIDVFAFGR
                     LDDGRLYLVMDLVEGRSLRAHLVDGPLPIDQALDILDTIADALDAAHDKGVVHRDLKP
                     DNIVLSSATPPKVFVLDFGIAKLVSKANEGDAKTGPGTLTGQGTWLGTPSYMAPEQWS
                     VDGAGPASDRYALGIIAFELLSGAPPFSATSVPGMMEQHFRAQVPALSTRGAIGVPPS
                     VDAILEKALSKDPDKRFASAKEFVGELRAAAGTGVHRARGAVEKSSTNRSMVPAIAGV
                     GVLGVAFAAYMATRGGDKPTVTPTPNEPAVQPGQISIEIVSSPPGAEVKTDHVVGTTP
                     MTLPLAPGVQVTLTVSKPGYLPDTKTVRAEPDGAHLFTLTQVTGFNGTWRMKNGELRV
                     FERTGDQVDVYKVTEVAGAREFFKHYKFTAIDKGIAFAAEDEVIDPRKPDDPSCHVPV
                     RVEYRYDPQADVLELRREKVNIDLHQGSCVVRARNIESDLLVHVDQAADEVVISAPAG
                     DPRKLKKQVLPKDPQADLEKKQKLEEAKKAEAAKNVKPTAKTNVGQKQAVSDKNADPF
                     GSAPQKGKASYSQIAPEPQVNAPMNTAEPQEQQQALPPPQQQKK"
     gene            complement(9092..9823)
                     /locus_tag="VMZ53_10845"
     CDS             complement(9092..9823)
                     /locus_tag="VMZ53_10845"
                     /inference="COORDINATES: protein motif:HMM:NF022041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUS29004.1"
                     /translation="MEQLVLYQPPRAWGTPNISPFCAKLETYLRITDVPYKIGPFSRS
                     KAPKGKVPFVDIGGTYIGDSQLIIEELEKRLAAEGKTPLDAGIAPRDHAIARMVRRTI
                     EEGYYFVGLYQRWSPDAGYELMKPAFKQFVPGLAIPLVRRMMRKKLHEQGTGRHTYDE
                     VMAIGAADLDALAELLGDRPFLLGDKPRTIDCTVFGFLEATLGFPLDGPLKKRGESHA
                     NLTAYRQRIRERWWTDLPASSGASA"
     gene            complement(9840..12431)
                     /locus_tag="VMZ53_10850"
     CDS             complement(9840..12431)
                     /locus_tag="VMZ53_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HUS29005.1"
                     /translation="MTTWTPPGEGGWRRDDAHVNTVMTGFADKLLHEAQAEGFAEGFA
                     HYGALLAGFDIQWVAGRVYMRPRIAGAPAQKIWGDKPPAKIKASAGKPPPKLIFKILM
                     KLHPELRKRARRADEVWSKKLWREMVARWKTELRPAFIAETRELTRVDVAALGDNALA
                     DHLERATSSLRQMIVQHFRHAPMQAVVVGDLLVRVGDWTGAKPAEVLGTLRGASPASR
                     AGADGEAAVAKAMANDAKSRALLDSDRSPAEIVAGLKAAPGAVGDAMRELLEVYGPRL
                     VTGLDLYHQTVEDLPEVIVKGIRAALANPHTEVAGDFAASLRERTPEAHRAELDEALE
                     DARLVYGVRDDDVGVLLWRLGVVRRAVHEATRRLETRGAVDAQEHALDALPDELLGLL
                     RGGTTPSAKDLAARTAKRHELAAIDPPVLLGVEGAPPPLDYFPPAVARMMRAVETFLT
                     CFHGERATGSTSADVVGTGVSTGTYEGRARVVRTMEDFAKIEQGDVLVARFTSPAYNV
                     LLPLLGAIVTERGGLLSHAAIVAREYGIPGVVDTRDALAKIPDGARVRVDGSTGTVTV
                     LAVATEGSGKRIAAPIVVVEAAYVEPATAGRVVSLREADATEFGGKAKALASAVAAKL
                     PVPDGVALDADLVARVVAGEADARARVTVGVAKLGGPWAVRSSAIGEDSAQASFAGQH
                     ATILGVQPDELFDAIAKVHASGHTASALAYRQKMGVTATARMGVVIQTLLRPDISGVL
                     FSHDPTAKTREGRLIEATWGLGEALVSGLVTPDRYRVGTDGKVIERAIGDKDIAIEAR
                     AEGGTAEVTIAADRAKTACLDDARLAELAQLANRCEQLFGGPQDLEWAVANGRLHLLQ
                     SRPVTTA"
     gene            complement(12428..13081)
                     /locus_tag="VMZ53_10855"
     CDS             complement(12428..13081)
                     /locus_tag="VMZ53_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS29006.1"
                     /translation="MNHIVTQDSATPAPTYAERRAAMADETRIRILDACVAILARGVA
                     ELSIPAVAREAGVSVPTVYRNFPDKKTLVAETAEYLRSRRGTYSTPTKLEDLADVIRK
                     NFEAAGSVNETVAAALQSEPILAMRRDSGMYQQRFAMMEALLHPEMEGASATDRENAV
                     MMAAVLCSSNTFRAFRDITGVSARRAAEVVAWTISRLVGRDSLESTKPARKAKGKAR"
     gene            13244..15610
                     /locus_tag="VMZ53_10860"
     CDS             13244..15610
                     /locus_tag="VMZ53_10860"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS29007.1"
                     /translation="MRFFEDPLDHATHVAAFLAESLRAGGTAGAIACPSVLDAIRDGL
                     ASHAVDVVAVLSQQRLVLLDAEEQLDALMAGAMPDASRFRAAIAPLVARLRERGSPVH
                     LYGEMVNVLTERGNSDAALQLEQLWSELAREQPFSILCGYNLRPLAGNVECLEKICEQ
                     HEGVARDTAQALAQLAERARALQTEVERRTRTEQRMHELLAVTSELAAATSRDEIARL
                     VVEVGRAAVGAESAGVWTVAGNAKELELASASLSSEHDAMKFTRVPLDGDTPAAHVMR
                     TGEPVFLASLHEYRAQFPESFERLRQLRDAYRAIAIVPMQAHGVTLGVMAYSYANEHT
                     FEDSERTFQRLLARQCALAFDRARMHREERTLREAAERLASAEKQARGDVELLYELIA
                     SVNRLDGIDDVYALVMRSVMRGTRSQRAAIMLFDETGAMQFEASEQLSREFKAAVAGH
                     TPWKREDFYPLPLAVDDCDTDAEWATLREAHRAEGIRALAVVPLINHQGQLLGQLMLY
                     RDDAQPFTGRDLQLASTVGVHVAQAVERTRKEREISRAYREEREARLLADEATRAREE
                     ILSVVSHDLRNPLGAILMCASSLLHVSADDSSGRIRTNVERIHRQAERMARYISDLVD
                     FAGIEAGRIRLERREHQVAEIVGAASELFGPIAAERGLKLETSILPDLPAIECDSERA
                     VQVLTNLVANALKVTPKGGAVSIGAETKDNSVVFYVRDTGPGIEQEELPNLFERHWRS
                     KSSNYKGAGLGLSIARGIVSAHGGKIWAESQLGAGATFYFSLSQLPTN"
     gene            15672..17990
                     /locus_tag="VMZ53_10865"
     CDS             15672..17990
                     /locus_tag="VMZ53_10865"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HUS29008.1"
                     /translation="MSAIAPLASTVVAVPRRLEVRRDAMVVEGHALEEVLLAGYALGP
                     ELGTAPVVLIVGGITASPFPFGGPDADAWWPSLIAADLIDPTRHTVLALCWPGNGSTW
                     RGFDDGALPPLSALGLADLIAAWLDGIGCAQPVIFAGASLGGLVGVALAVRHPERVAK
                     LLTISAGLRPDGWGTATRHLQRELVRDGLRTGDATTGMIRARQLGMLTYRGRDELDTR
                     FGVLSPGLEQPPVAAYLDHHGQRFAQRFPLRTFLLLSEAIDRSRFASDAAGVRDALAR
                     VTADVLVVGVPGDLLFPFPLQNELYRELQAAGASASLWKLDSEYGHDAFLADQDKLAE
                     LIRDSGFLQAAEPRPRFHGVGAKPLREIRIGMIGCGTVGNGVLDLLDKQKAQLAERYS
                     VRYRVTRIAVRDATKPRGPLAKDIPLTTRALDLVADPNVDVIVEVAGGDAVEPALTAA
                     LAAGKPVVTANKALLARQLARLGMLAQRTETPLLCEAAAAAALPIIRHLSHRADEVDA
                     LQAIVNGTCNFVITRLEQDELTLDRAIAEAQSLGLAEADPSADIDGHDAAAKLSILAY
                     RAFGAWVPPDALPVRGIGELWPADCDLAEAMGFRIRLIAQAARVTGGLTAAVEPVLLP
                     DWHLLASVEEEYNAVYMKTASSGDLSLFGKGAGALPTASAVLGDLIDLAQDNSVQWPT
                     PRDIALVPAPARRHYIRVSAEPHPGLTRRVDSIVRRAGLTVQNRAARGEPLITHHGFL
                     ISPSDDATIRGVIDQLRELGRVEQTLWLGVVE"
     gene            18017..19237
                     /locus_tag="VMZ53_10870"
     CDS             18017..19237
                     /locus_tag="VMZ53_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUS29009.1"
                     /translation="MGAATRALHDAVDANAPYLHPELRTIQRDLRDSSNVVSWQEAKA
                     AFIVTGDGAWSELPHLYARYGTPGTSELIAELRKLEHARCTFVTDCGMQAVALVADVS
                     IAHGAHVIAMRQVYNKTRAFFEATCNRVGATLTLVDDGDLTALAAALRAETRFVFAET
                     FTNPLGRAQDIPAIVALVRSRAPSARLVIDSTIATPWGVRTPLLDQGVDIVVGAMTKA
                     LGGQDAALGGYIATNHADAGNQIMDLIAMRGGILDDERARRVVAHLPQAERKHARRCE
                     TAASVAAFLASHPRVEAVFHPSRPDHPDAAVIARDYTRTGSLLAFRVRDLDEAAHGRL
                     ADVLVSCGILRYALSFDGLVTKVNHHKTVSEYFTPAENVARLGIQRLIRLGIGLEDAE
                     DLIACLDWTLAQAA"
     gene            19352..19897
                     /locus_tag="VMZ53_10875"
     CDS             19352..19897
                     /locus_tag="VMZ53_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29010.1"
                     /translation="MIAWPAHSPRELLRYPFSPAEFQAVQTWFFVHHLAMVAVMLGFA
                     RSGAMGTGRIMRGAAWVAAIGVALLAVNELNAIRYAEWTLHTANAGALGAGYGISTNL
                     VGLGTLVAGIGVLRAKRWTGWHRWVPLAIALVHFVGVTPALFSESFVGARIAIVTWVA
                     LFGALGAALISETRRAAASSN"
     gene            complement(19894..20967)
                     /locus_tag="VMZ53_10880"
     CDS             complement(19894..20967)
                     /locus_tag="VMZ53_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29011.1"
                     /translation="MTASPSKRWLFSAPIDVAVFGGTAVIALALVALAPSLGIGRESP
                     EWSWIAGVLLVDVAHVWSTSFVVYFDPVEWRRRPMLYALTPLAMFGVGVALYAWGPGP
                     FWRAIAYLAVFHFIRQQYGWVMLYRARNGERDALGRFLDGATVYLATLYPLIWWHAHL
                     ANRDFAWMKKGDFIGGLPTWVADLAGLVYALLLVIYFARAGMQIVRRESVSWGKHLVV
                     ATTAACWYVGIVSSNTDFTFTITNVFIHGIPYMALVYIYARNGARDPDAHDGPNHRIF
                     TRGRGLAIFLATLWFIAYAEELLWDRSLWHDREWLFGGSFDIDNAAVIIAPLLAVPQL
                     THYFLDAFLWRRTANPRLGKLLG"
     gene            21129..23237
                     /locus_tag="VMZ53_10885"
     CDS             21129..23237
                     /locus_tag="VMZ53_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HUS29012.1"
                     /translation="MYLLRFGLLAALAATACHESAGGDDDATPLECPAETATTGALAL
                     DGVDDYARTPKVVPALGLSTFTIETWVRRDGEGVTYTTGAGGLRLVPIAGKGLGEGDK
                     SNVDCNYSVGFWGDVLGADFEDMATGANHPVMGKTAVPRGEWHHIAATYDGAAWRLYL
                     DGKLDAEAVANATPRADSIHPFGIGTAIDSAGAPHGFLAGALDELRVWDRARTPEEIA
                     DGMYRSIAMADGLVARWAFDAGGVDPAVADDSVHTAPVALTGGAEVSVADVAALDHGS
                     APVVAAVSPADGAASAPGVVLDVSLEMESRAPVDVTYHVRELTDADDFTIVVLPDTQI
                     YTLEGRNLEKYFHDQTQWVRDNRERYNIVGVIHNGDVINNEPQIYQWEVASAALARLE
                     KPEPALPDGMPWGVSVGNHDNKLIGANTVLNTDKFNQYFGVNRFAGRAYYGGHYGTKN
                     DHSWVTFSAGGLDFVVVSLMYELDPDPAAIAWARSIFQMHPNAFGILNTHYLLTASGR
                     FSPQSQQIYAALKDLPNVQLMTGGHVAAESRRVDAFEGHTISSMLADFQGRDLGGGGF
                     MRIWEFSPASQRLSVRTYSPTMDKFETDADSEFTLDVDLRGAGGPFRTLQVKAADPAH
                     VALPVDGLEVGKSYEWYAEVASCGHRMATPLYRFTTMASARAAQQFNDTLYQPSGRDR
                     TKRVTSGVVNAQPDDPSLVD"
     gene            complement(23250..24062)
                     /locus_tag="VMZ53_10890"
                     /pseudo
     CDS             complement(23250..24062)
                     /locus_tag="VMZ53_10890"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
     gene            complement(24059..24934)
                     /locus_tag="VMZ53_10895"
     CDS             complement(24059..24934)
                     /locus_tag="VMZ53_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUS29013.1"
                     /translation="MPRVSRIAREPLVHFLAIGAALFALDAWRSEPTPRTSPPPSSST
                     PSSATPIADAGVAERRIVVDPAVRAASAEAAERRLGRAPTPAEIADETERWIDEEILY
                     REAVSRGLDRDDPVIHQRIAGRMSYVLEQALVVPEPTDAELRAWFDSHREQWSVPEHV
                     DFTHVFVTGDNATARADELLTQLANGAPPERLGDRFPAGHKFRGRKLADLAQSFGAEF
                     VDGLASQSPGAWVKRTSRHGVHLVRVDRVDAPRDIDFAAAKLDVRKAWLDAKKEELVR
                     NAMKQLRATWTVEQR"
     gene            24980..26296
                     /locus_tag="VMZ53_10900"
     CDS             24980..26296
                     /locus_tag="VMZ53_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29014.1"
                     /translation="MKFLTCALLCMLACGSPQPAPVAPPLSGSAAAPEAPPVATPVAI
                     AFVIEGWEMWVGNDQIPDIDEREQYQGALQPFKEAFGRLKLTDFPAGSQAAVVTYTDR
                     ATVRQPMQPIDKLTPAAFGEQKDYFGTIDRDLVSGVTAGLDELAKAKNARRILVVIGD
                     GNDSHPSTAKAALGELAKRAAAEKVEVVSLRYLGPLSSPSSNVSAFDPATLTINSIAG
                     ITDQLDFLFTRLKKPPVAAASTTAIKAPLVVAFLVNGSEVWMGNDDVEPQDSPARYVG
                     ALKPMRAAFEKAALTGFPAGSVAMLITYDSATKVRVPPMAIENFGARALGAQGDYHGG
                     FGTELVSGVTGAFTELLKIDAARRVVVVIGDGNDTNNDTAKTQLQGLALRAAEYHIEV
                     HALVFKGQLSEPGTVVTALVPNAETAASADDLTAKLVTLFSTLRKK"
     gene            26301..26660
                     /locus_tag="VMZ53_10905"
     CDS             26301..26660
                     /locus_tag="VMZ53_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29015.1"
                     /translation="MRRLFVISLLVVGSIAYADKPKARKPVRPVPQNAIVGRIVGLDI
                     YTDEGRDSSVITVAAGSENGISKGWHARFREGTTSNRLSGGDATVIRIDRRTVVLKTD
                     LPAEQVRANRYIEFSPR"
     gene            complement(26657..>27597)
                     /locus_tag="VMZ53_10910"
     CDS             complement(26657..>27597)
                     /locus_tag="VMZ53_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS29016.1"
                     /translation="DARGLTSERLRWLCDYACRDDYALTAAQTSAWAGLFYFAGRQSA
                     PGAEAQSVVTWPDGNNALITHLAVGATIERGIAVVDIDEVTGRIVAVGPSGPLGIEAD
                     KIIVATPSFVANRIVRGRRPITPHYVPPPVAHRSGPDYGAWVVANVHLRGRPIERGRG
                     APPAWDNVFRDSPSLGYVSATHQRGRDRGPTVWTWYYPFTDEDSTSVRKKLAGAGYAE
                     WAETILADISRAHPDVRDHVEHIEVAFWGHGMIRPRVGSIWKQSLESLVARGGDRTPI
                     GRVHFAHTDLSGIALFEEAFDHGLRAAREVVEGLRA"
BASE COUNT         4518 a         9253 c         9217 g         4609 t
ORIGIN      
        1 gcccagggcg cgtgaacgcg gctgccgaat ggcgagagga tgcagacgcg gatgtcgccg
       61 agctcgtcgc ggaagcgctc gatcgtgatc gcgcgatcgg tcggcacgca gccggcttgc
      121 gcgcgctgat cggagaggta gcgcacgaga ttgcccgcgg cgagatcgtc gaggttgtat
      181 tcggattgga gccagctctg cgcggctggg agatccgcaa cgagcttgcc gtcgagctcg
      241 cgacagaatg cgccgagggc tttgccgagc tcgactggac ggcccggtcc gtcggcacgc
      301 cagaacggca tcttgcccgg ctcgcccggc gcggggctga cgaccacgcg gtcgcgcgtg
      361 atgtcctcga tgcgccacgt cgttgcgccg aggatgaacg tctcgccttt gcggctctcg
      421 ttgaccatct cctcgtcgag ctcgccgatc tttgggccgc gctcaccgag gtgcacgccg
      481 tagaggccgc ggtcgggaat cgtgccgccg ctgacgaccg agagcaggcg cgcgcctttg
      541 cggccgacga ggatgtcggc ttggcgatcc cacgtgagcc gcggacgaag ctcggcgaac
      601 tcatcggagg gatagcgacc ggcgagcatg tcgagcacgc cgacgaatgc tgcatgcgag
      661 agatcgcggt agctcgccgc gcgccgcacg atgcgctcga gctcgccgac ttgccacgga
      721 tccgcggcga ccatcgcgac gatctgctgc gcgaggacgt cgagcgcgct gtccggaacc
      781 cggatagctt cgacgttgcc agcgagcatc tcgtgcgcga cgacagtcgc ttcgagcaag
      841 tcgccgcgaa acttcggaag gatgcgcgcg atgctcgtcc cgccgacgtg gtgaccggcg
      901 cgccccacac gctgcaggcc gcgagagacc gcgccgggcg actcgacctg caaaacgagg
      961 tcgactgcac ccatgtcgat accgagctcg agcgagctgg tggcggtgat cgcgcgcagg
     1021 cggccagcct tcagcgcttc ttcgatctcc atgcgctgat gccgcgcgac ggagccgtga
     1081 tgcgcgcgca cgagctcgac gccaggttcc gcatggcctt gctgaacggc gagctcggtc
     1141 agctgctgcg cgacgcgttc ggcgaggcgt cgcgagttga cgaacacgat cgtcgaacgg
     1201 tgagccagga tctcggagag cagcttcgga tagatgagcg gccacatgcc gccgccatcg
     1261 gtcgcgggcg ccgcgttctc tttttggaat gcgttcggat tgggcttggg cttcgcggga
     1321 gcagggcccg gattgtccat gtccgcggca ggcacgacga tctggagatc cagtgcgggc
     1381 cgcgcgctgg cgtcgacgat ctcgacgggc cggtcgcctc ccagatatcg agcaacttcg
     1441 ataagtggac gttgagtcgc cgacaggcca atgcgctgcg gctcgggatt tcccgacgac
     1501 gtgacgaggt agctcagccg ctcgagcgtg agcccgagat ggatgccgcg cttcgtcggc
     1561 gcgacggcgt ggatctcgtc gacgatgatc gtatcgacgt tgcgcagccg ctcacgcgaa
     1621 cgcccggcga ggatcaagta cagcgactcg ggcgtcgtga tgaggatctg cccaggatcc
     1681 ttgcgctgcc gctcgcgctc cttttgcgac gtatcgccgg tgcgaacatc gacggtgatc
     1741 gccgcgccgg cgccgaggcg ctgcaggccc gccagtggcg cgcgcaggtt ccgctcgacg
     1801 tcatacgcga gcgccttgat cggcgagacg tacacgacgc gagtgccttc cgacggctcg
     1861 cggctcagcc gatcgaggca ccatagaaac gccgcgagtg tcttgccgct gccggtcggc
     1921 gcgagcatga gcgtgtgctt gccgctcgcg atgctcgacc agccgcggtc ctgcaccggc
     1981 gtggcttgcg cgaagctctg cgagaaccac gtacgcgcgg cttccccgaa tggcgcgagc
     2041 ggatcagccg gcacctccgc tctatagcaa caacgaccgg ctcaagcccc cagctgatgg
     2101 gccagtgcgg cccacggcgc gaggaaagct gacatgacgt tggcaagtgg cgacccgaac
     2161 cactgctgga ttccaggatc cacggagggc gtgttcaagc cgatcaccat gccgcgaggc
     2221 cacggctgcg catcaggatg cttcggcggc acgagcacgt cgaagtgctg cggcgcgagc
     2281 tgcacgcgat cccacggcgt taaaacgatc gagtcgctgc cctcggacgg cggggtctcg
     2341 aacgccaggt cggcgcgcgg cgcacgaacc gtgtcggtcg cccaaacaat gtcggcggcc
     2401 aacggtccga tgcctcgctg ctgatggtgc tcggcccaag gcacgcaggc gatcgcagtc
     2461 aacagcaggg cgccggccaa cgagggcctg atacgggcgg caatgctgaa catgcccgag
     2521 caaccagcct cggccgtcag gacattcccg cgcgccgccc gtgatcggcc gtccaagacg
     2581 ccggccctca gtcgtcgcgg tcgaggatcg cgccgaggag cagcggcccg agcgtcgtgc
     2641 cgagcgtgat gccaatcgag agcgccgggt tcgcgctgat cgtcgcctga acgcgcttga
     2701 tgaagctccc gatcgctacc gattgcagcg cggacatgac gagcacaccg acgccgccac
     2761 cgacgacaat cgccgcaaac ccgcccgcca aatcgggcaa tttgcgaagc ggcgccgtta
     2821 gcacgcgcag gatcggaatc aggacgcctg ccgccgccaa atagaacggg aaaaacatgc
     2881 cggcggtaaa catcgcgaac cacatcgcgc gggtctgtgc cggaaacgag gcgggcgggg
     2941 tcggattgag cggcccgatc gagaccttcc aggtctggaa cgcgacgcac gtcagataga
     3001 taaacgcgaa cgtgaaggcg agcttcacgg gcacggattt gatgcgcatc caaaggggca
     3061 ggccgtcgag cgtcgactcg tactgaaggc ggccttcgaa gtaaccggcc acgaagatca
     3121 gcggcagcac ggccagcatc acgccaagcg gaacgcccgc tggaagcttg gcggccacca
     3181 agtcgaagcg cgtcgccacc gcggctgcgt tgatcaacgc gaccagcaga aagaacggcg
     3241 cgagctttcg cggcgacact cgtccagcgt atcggatgag actcagcagc cagcgtttcg
     3301 aggcacgcga agccagcgcg tcttcgcggc cgcagctcgg acatctggtt gcggaagttg
     3361 cgtccgtccc ttaagcggct gccgcaacct ggcgaccgca agtccgcatg attccagcgt
     3421 caggcatacc ttggagttga cataagaccg attctgcgac gcgaccccta tgtccagccg
     3481 ccgcccgcct ctatgccaga ctgggctccg aaccgcgggc ctcgacccgg atcgaacgcc
     3541 agacttaccc cggaacgtag atggtctcgg tagccctcat cgcaacgatc ctcgccgtgg
     3601 ttgcgggcgg tcgcgcgctg catcggcatg aacgccggaa tcccaacgag cgtatgcggc
     3661 ggctcttgaa gtcgaccaag cgcgtgcgga ttgccgagct tccggagagc caggttggtt
     3721 gcaccatcgg tcgcactcgg ccgctcgaga tgacgagctt ggtcgaggcc gcgctgaccg
     3781 gccggccgtg ctttttctat atcgccgagc tcgaggttcg ggccggcagc ggctggcagc
     3841 tcgttgcgcg cgaacgcgtc ggcggcccgt tcatcattca ggacgccagc gggcgcgcgg
     3901 tcgtggagcc ggacggcgcg cgcctcgata tcgcctttga ttttgtcgac cacgtttggg
     3961 gtaatcccac tgcgcgcgag catcagttca tcttgcgcgc gaagcccgat gccgttgggc
     4021 tgccgttcac gcatcaggtc cggtttcgcg aagcggtcat cggcgcggac gagctcgtgt
     4081 ccgtcttggg cgctggcgtc cgcgaagccg acccactcgc tccgcccgtt gcgagctacc
     4141 gcggcgagcc ggtgacgcgg ctgcggtttt cgagctcgcc ggatgcggag ctcctggtca
     4201 gcgcggccaa ggagaccatc atcgggcgcg attcgatagt cacttcgata agcggtggtg
     4261 gtgctagcga gccacgcgtc ggcgatggcg gcgccggtgc cggcaccaag gacgacgatt
     4321 agccgcgcgc gcgactggtc gctagccgat tgccgagctg gttgatctcg tcctgcgtca
     4381 agccgctcga cgcttcgatc gtgacggtcg acgcgcgacc cgtccctact tcgaccgcgc
     4441 tcacttcgag gatgccgtcg gcgtccatcg tgaaggcgac ctcgactttt gtgcgaccgc
     4501 gtggcgccgc tgcgagatcg ccgagcacga agcgaccgag gcgtcggttc ttcgacgcgt
     4561 tctcgttctc gccttggtag acctcgatcg cgacgaacgt ctggttgtct tccgtggttg
     4621 cgaagacctt tttcgcgcgc gtcgggatgc tcgtgttgcc cgggatgacg accgacatct
     4681 tgtcgcccgc gactttgacg cccatgttgt gcggcgtgac gtcgagcagc acgacctctt
     4741 gcagctcgcc acccatgatg ccggagtgaa cggccgcgcc catcgcgacg atctcgtcgg
     4801 ggttgacgcc cttgctgccg ggcttgccga agatgcgttc ggcgtattcc tggatggccg
     4861 gcatgcgcgt catgccgccg acgaggatga cctgatcgat gtcttcgggc gtgcagcggg
     4921 cgtcggcgag cgcgcgagta cacggcgctt cgaggcgttc catcagcggc ttgcacgcgc
     4981 gttcgagctg cccgcgctcg agctcgcgca tgaggtgcat cggcccgtcg gggccagcgg
     5041 cgataaacgg caagttcacg tccgtcgtca tcgcgctcga cagctcgatc ttcgcgcgct
     5101 cggccgcctc tttgagacgc tgcaatgcga ccgagtcgcc gcgcaggtcg atgttcgtca
     5161 tgccttggaa ttcgtcggca agcagatcga tgatgacgcg atcgaagtcg tcgccgccga
     5221 gcgcggtgtc gccatacgtc gagagaactt cgaacacgcc gttctcgatc gcgagcaccg
     5281 agatgtcgaa cgtgccgcca ccgagatcga agaccgcgat gcgctggttg ctcttgttgt
     5341 ggaggccgta cgcgagcgcg gcagcggtcg gctcgttgag gatctggcgc accgtgagcc
     5401 cggcgatctt gccggcgtct ttcgtcgctt ggcgttgcgc gtcgtcgaag tatgcgggca
     5461 ccgtgacgac ggcttcggtg acgggctggc ccaggtagtc ctcggcgacg cgcttcatct
     5521 tcgcgagaac atgcgccgag atctcctgcg gcgagcgcgg cgtgccttgg atgtcgaccc
     5581 agcagtcgcc gttgcgggca gcgacgacct tgtatgccag gcggcgtgcg agcgaaagga
     5641 cgtccggatc cgtcgccttg cgaccgatga gccgcttcac gccaaagacg gtgcgctgcg
     5701 ggttcgtcac catctgccgc ttgctcgcgg caccgacgac gacttcaccg tcggcgttcc
     5761 acgacaccat cgacggcgtc gtgcgcccgc cttcttggtt gtggatgacg atcggctcgc
     5821 cagcttccaa cacggccacg cacgagttcg tcgtgcctag atcgatgccg atgacgcgcc
     5881 ccatcgctcg tcggcgacta tagcattgac ctagcggcgg cggcctagct cggccagcgc
     5941 ttggttcgcg agatcgagat ttggatccag ttggagcgcg cgctggtact cgagcgtggc
     6001 ctcgtcgggg cggcccgcgg catgtgcttc acggccacgc gcaaggcaaa ggagcgcgcg
     6061 atacgacttc gactgcggaa tcttcgccgc caggttgtgc agggcctgcc gcgcatcgcc
     6121 ccaccgtttc tcgttgagca tcatcatcgc ttcacgcagc gcgctgcgct catcgaacgc
     6181 cggcgccggt tgcacacccc ggacggtccc gggattgaga tcggcggacg gcttgggcgg
     6241 catcatcggc gccgtggacg gacgcgtcgc cgccggcgtt gtcggacgaa ccggcgtggt
     6301 cggccttgcg gccacgggcg tcaccgggcg ctgccccggt gtcggcggac gcatgccttg
     6361 cgcgggcgtc ggtggacgct gcatgccttg cgcgggcgtg ttcatcggcg gcgtcatcgg
     6421 acgctgcatg ccatgcgccg gcgtgttcat cggcggcgtt gcgggtcgcg tgccctgcgg
     6481 ttggatcacc ggcgtcgacg gacgctgcgg gactccggcg cgatccgttc cacgcgcgag
     6541 ctgctgcgtc tgcgtcgtgt tgaccggcgg ctgcgtgccg agctgggcgc gaccttggcc
     6601 tgtcgtgcgc gcttgatcga tcggcacctg cccggtacgg cgcaacaggt gcggcgcggg
     6661 cgttcgaccc gtccctgcac cgtcggtcat caccgggatc gcttggctac tgctcgccga
     6721 gccgagcccc ggccgcgtgt gcgttccgcc gagagcgcgc tgcagctgat cgtgcgccga
     6781 cttgatgccg agaaagacct cgttcgacag cctctgcaga tcagtcgaca tgcggccgaa
     6841 gcgcgccgga tggaattgct tcgtcagcga gagaaacgcc gcgcgaatat cttccgtcgt
     6901 ggcgtactcg ctgattccga gcgcgctgta cggcccgtgc gagatgcgca attgcatcgc
     6961 gtcgagcgca gctcgagccc tgagctcctc gtcggccact gtcaatcatc gtagctcgaa
     7021 cgcgaggacg agaaaagaac gtcacttctt ctgctgttgc ggaggcggca gcgcctgctg
     7081 ctgctcttgg ggctcggccg tgttcattgg cgcgttcacc tgcggctctg gcgcgatctg
     7141 cgagtacgac gccttgccct tttggggggc ggagccgaac gggtcggcgt tcttgtccga
     7201 caccgcctgc ttctgcccga cgttggtctt ggcagtcggc ttcacattct tcgctgcttc
     7261 cgccttcttc gcctcttcga gcttctgctt cttctcgagg tcggcctgcg ggtccttggg
     7321 gaggacctgc ttttttagct tccgcgggtc gccggctgga gccgagatca cgacctcgtc
     7381 cgccgcttgg tcgacgtgta cgagcaggtc gctctcgatg ttgcgggccc tcacgacaca
     7441 gctgccctgg tgcagatcga tgttgacctt ttcgcggcgc agctcgagca cgtcggcctg
     7501 cgggtcgtag cggtactcga cgcggactgg cacgtggcag ctcgggtcat ccggtttgcg
     7561 cggatcgatc acttcgtctt cggctgcgaa cgcgatgcct ttgtcgatgg cggtgaactt
     7621 gtagtgcttg aagaactcgc gagcgccggc gacctcggtg accttgtaga cgtcgacttg
     7681 atcgccggtc cgctcgaaca cgcgcagctc accgttcttc atgcgccacg taccgttgaa
     7741 cccggtcacc tgcgtgagcg tgaagaggtg tgcaccgtcg ggctcggcgc gcacggtctt
     7801 cgtatctggc aagtagcccg gctttgacac ggtcagcgtg acctggacgc cgggcgcgag
     7861 cggcagcgtc atgggtgtcg tgccgacgac atggtcggtc ttcacttcag cgcccggcgg
     7921 tgacgaaacg atctcgatcg agatttgtcc gggctgcacg gcgggctcgt tcggcgttgg
     7981 cgtgacggta ggcttgtcgc ctccgcgcgt cgccatatac gcggcgaacg caacgccgag
     8041 cacgccgacg cctgcgatcg ccggaaccat gctgcgattc gtcgacgatt tctcgacggc
     8101 gccgcgcgcg cgatgaacac cggtgccggc tgccgcgcgc agctcaccga caaattcttt
     8161 cgccgacgcg aagcgcttgt cgggatcctt cgacagcgcc ttctcgagga tcgcgtcgac
     8221 cgaaggcggg acgccgatcg ctccgcgcgt cgagagcgcc ggcacctgcg cgcggaagtg
     8281 ctgctccatc atgcccggca cactggtcgc cgagaacggc ggcgcacccg acagcagctc
     8341 gaacgcgatg atgccgaggg catagcgatc tgatgcgggc cctgccccgt cgacactcca
     8401 ctgttccggc gccatgtagc tcggcgttcc gagccaggtg ccctgccccg tcagcgttcc
     8461 cggtcccgtc ttcgcgtcgc cttcgtttgc cttcgacacg agcttcgcga tgccgaaatc
     8521 gagaacgaag accttcggcg gcgtcgcgct cgagagcacg atgttgtccg gcttgagatc
     8581 gcgatgcacg acgcctttgt cgtgcgccgc gtcgagcgca tccgcgatcg tatcgaggat
     8641 gtcgagcgct tgatcgatcg gcagcgggcc atcgacgaga tgcgcgcgca gcgagcgccc
     8701 ctcgacgaga tccatcacga gatacagccg gccgtcgtcg agccggccga acgcgaacac
     8761 gtcgatgacg ttgtcgtgat cgacttggtt caccgcgcgc gcttcgcgaa tgaagcgctc
     8821 cgccgcctcg gccgaagccg cgagctcgtg ccgcagcact ttgatcgcga cacgcttgcc
     8881 gatgaccggg tgcttgccgg cgtacacctc gcccatcgcg ccggaaccga tgaagcgatc
     8941 gacgacgtag tcgccgacct tcgtgcccgc gacgagctgc tccgtgccgc catgcggctc
     9001 ggccgcggag agcgtcgggc tgtgcgccag cggggacgaa tccgccatct ccctcatcgt
     9061 agcagcggga caccccgact gccggtcagt ttcacgccga tgcgcccgaa gaggctggca
     9121 aatctgtcca ccatcgctca cgaattcgct ggcggtacgc ggtcaaattc gcatgggatt
     9181 cgccacgctt cttgagtggg ccgtcgagcg gaaagcccag tgtggcctcg aggaacccga
     9241 agaccgtgca gtcgatcgtc cgcggcttgt caccgaggag gaaaggccgg tctccgagaa
     9301 gctcggcgag cgcatcgagg tcggcggctc cgatcgccat cacctcgtcg taggtgtgcc
     9361 gtccggtgcc ctgctcgtgg agcttcttgc gcatcatgcg tcgcacgaga ggaatcgcga
     9421 ggcccgggac gaactgcttg aacgcgggct tcatcagctc gtagcccgcg tcgggactcc
     9481 accgctgata gagaccgacg aagtagtagc cctcctcgat cgttcgccgc accatgcgag
     9541 cgatcgcgtg atcgcgcggc gcgatgccgg catcgagcgg cgtcttgccc tcggcggcga
     9601 gccgcttctc gagctcctcg atgatgagct gcgagtcgcc tatgtacgtg ccgccgatgt
     9661 cgacgaacgg gactttgcct ttcggcgcct tgctgcggga aaacgggccg atcttgtacg
     9721 gcacgtcggt gatgcgcagg taggtctcga gcttcgcgca gaacggactg atgttcggcg
     9781 tgccccatgc gcgtggcggt tgatagagaa cgagctgctc cacgcatgga gcgtagcgtt
     9841 tacgccgtcg tcacggggcg gctctgcaac agatgcaggc ggccatttgc cacagcccac
     9901 tccagatcct gcgggccgcc gaacagctgc tcgcagcgat tcgcgagttg cgcgagctcc
     9961 gccagacgcg cgtcgtcgag gcacgcggtc tttgcgcgat ccgcggcaat cgttacctct
    10021 gccgtgccgc cttcggcgcg cgcttcgatc gcgatgtcct tgtcaccgat cgcgcgctcg
    10081 atcactttgc cgtcggtgcc gacgcgatag cggtcgggcg tgacgagccc cgagacgagc
    10141 gcctcgccaa ggccccacgt cgcttcgatg aggcgccctt cgcgcgtctt cgctgtcggg
    10201 tcgtggctga acagcacgcc cgagatgtca ggacgcagca gcgtttggat cacgacgccc
    10261 atgcgcgccg tcgctgtgac gcccatcttc tggcgatacg cgagagcgga agcggtgtgg
    10321 ccgctcgcgt gcaccttcgc gatcgcatcg aacagctcgt cgggctgcac tccgagaatg
    10381 gtcgcgtgct ggcctgcgaa gctcgcctgc gccgagtcct ctccgattgc cgagctgcgc
    10441 accgcccacg gcccaccgag ctttgcgacc cccacggtca ctcgcgcgcg tgcgtccgct
    10501 tcgccggcaa caacgcgtgc gacgagatcg gcgtcgagcg ccacgccgtc gggaaccggc
    10561 agctttgccg caacggccga cgcgagcgcc ttcgctttgc cgccgaactc cgtcgcgtcg
    10621 gcttcgcgca ggctgacgac gcgacctgcc gtggcgggct cgacgtacgc agcctcgacg
    10681 accacgatcg gcgcggcgat ccgcttgccg cttccttcgg tcgccaccgc gagcactgtg
    10741 acggtgccgg tggatccgtc gacgcgaacg cgcgccccgt cgggaatctt cgcgagtgca
    10801 tcgcgcgtgt cgacgacgcc gggaatgccg tactcgcgcg cgacgatcgc cgcgtggctc
    10861 agcaggccac cgcgctcggt cacgatcgcg ccgagcagcg gcaacagcac gttgtacgca
    10921 ggcgacgtga agcgcgcgac gagcacatcg ccttgctcga ttttggcgaa gtcctccatc
    10981 gtgcggacca cgcgcgcacg accttcgtac gtaccggtgc tcacgccggt gccgacgacg
    11041 tctgcgctgg tcgacccagt cgcgcgctca ccgtggaagc acgtcaagaa cgtctcgacg
    11101 gcgcgcatca tgcgagcgac cgccggcggg aagtaatcga gtggcggcgg tgcgccttcg
    11161 acgccgagca gaaccggcgg atcaatggcg gcgagctcgt ggcgtttcgc cgtgcgcgcg
    11221 gcgagatcct ttgcgctcgg agtcgtgcca ccgcgcagca gaccgagcag ctcatctggc
    11281 agcgcatcga gtgcgtgctc ctgtgcgtcg acagcgccgc gcgtttcgag gcggcgcgtt
    11341 gcttcgtgaa ccgcacggcg cacgacaccc aggcgccaca gcaggacacc cacgtcgtcg
    11401 tcgcgaactc cgtagacgag gcgcgcgtct tcgagtgctt cgtcgagctc ggcgcggtgt
    11461 gcttcgggcg tgcgttcgcg cagcgatgcc gcgaagtcac cggcgacctc cgtgtgcggg
    11521 ttcgcgagcg cagcgcggat gcccttcacg atgacttcgg gcaaatcctc gacggtctgg
    11581 tgatagaggt cgaggcccgt cacgagccgc gggccgtaca cttcgagcag ctcgcgcatc
    11641 gcgtcgccca cggcgcccgg cgccgctttg agcccagcga caatctcggc cggcgagcga
    11701 tcgctgtcga ggagtgcacg cgactttgca tcgtttgcca tcgccttcgc gacagccgcc
    11761 tctccatctg cgccggcgcg cgatgcggga gatgcgccac gcagcgtgcc gagtacctcc
    11821 gccggcttcg ctcccgtcca atctccgacg cgcacgagca gatcgccgac gacgaccgct
    11881 tgcatcggcg cgtggcgaaa gtgctgcacg atcatctggc gcagagagct cgtcgcgcgc
    11941 tcgaggtgat cagcgagcgc gttgtcgccg agggcagcaa catcgacgcg cgtgagctcg
    12001 cgcgtttcgg cgatgaacgc cggacgcagc tcggtcttcc agcgagcgac catctcgcgc
    12061 cacagcttct tgctccacac ttcgtcggcg cggcgtgctc gcttgcgcag ctcggggtgc
    12121 agcttcatca agatcttgaa gatgagcttc ggaggcggct tcccggcgct cgccttgatc
    12181 ttcgcgggcg gtttgtcgcc ccagatcttc tgtgctggcg cacccgcgat cctaggacgc
    12241 atgtacacgc ggcccgcgac ccactgaatg tcgaagccag cgagcagtgc gccgtagtgc
    12301 gcgaagcctt ccgcgaagcc ttcggcctgc gcctcgtgca agagcttgtc ggcgaacccg
    12361 gtcatcaccg tgttcacgtg tgcgtcgtcg cggcgccagc cgccttcacc aggtggagtc
    12421 caggttgtca tcgtgctttg cctttagctt tgcgtgcggg tttcgtggat tcgagggagt
    12481 cgcgaccgac gaggcgcgag atcgtccacg cgacgacctc ggcggcgcgg cgcgctgaca
    12541 cgcccgtgat gtcgcggaat gcgcggaacg tgttcgagga gcagaggacc gccgccatca
    12601 tcacggcgtt ctcgcggtcg gtcgcactcg cgccttccat ctcgggatgc agcagcgctt
    12661 ccatcatcgc gaatcgctgc tgatacatcc cagagtcgcg acgcatcgcg aggatcggct
    12721 cgctctgcag cgcagcagcg acggtctcgt tcaccgagcc agccgcttcg aagttcttgc
    12781 ggatcacgtc cgcgagatcc tcgagcttgg ttggcgtcga gtacgttccg cgacggctac
    12841 gcaggtactc cgccgtttcg gcgacgagcg tcttcttgtc ggggaagttg cggtagacgg
    12901 tcgggaccga cacgccggcc tcgcgcgcga ccgcggggat cgacagctcg gcgacaccgc
    12961 gcgccagaat cgcgacgcat gcgtcgagga tccggatgcg agtctcgtcg gccatcgcgg
    13021 cccgccgttc cgcgtaggtg ggcgctggag ttgcgctgtc ctgagtcacg atatggttca
    13081 tctcatgacg atatgatcca tgtcaagcag aacgtgacag ccgcgctcgg ttgtggcgcc
    13141 accgcacgga gacagatcgt cttaaaaatc aggcacctca gtcactctcg atggtgctaa
    13201 cgctttcgtg atggggttgg gttcggaaag gcggcagcat cgtgtgaggt tcttcgagga
    13261 ccctctcgat cacgccacgc atgtcgccgc gttcctcgcg gagtcgttgc gagctggcgg
    13321 aaccgccggt gcgattgctt gcccttccgt gctcgacgcg attcgcgatg gcctcgcttc
    13381 gcacgccgtc gatgtcgtcg cagtgctctc gcaacagcgt cttgtcctgc tcgacgccga
    13441 agaacaactc gacgcgctga tggccggcgc gatgccggat gcgtcgcggt ttcgcgccgc
    13501 aatagcgccg ctcgttgctc gcctgcgaga gcgtggctcg ccggtccacc tctacggcga
    13561 gatggtgaac gtcctcaccg agcgcggcaa ttccgacgcg gcgctccagc tcgagcagct
    13621 atggagcgag ctcgcgcgcg agcagccatt cagcattctt tgtggctaca acctccgccc
    13681 gctcgccggc aacgtcgagt gtctcgagaa aatctgcgag caacacgaag gcgtcgcgcg
    13741 cgacactgcg caggcccttg ctcagctcgc cgaacgagca cgcgcgctcc aaaccgaggt
    13801 cgaacgccgc actcgcaccg agcagcgcat gcacgagctg cttgccgtca cgagcgagct
    13861 cgccgcggca acgagtcgcg acgagattgc acgcctcgtc gtcgaagtgg gccgcgccgc
    13921 cgttggcgca gagtctgcgg gcgtgtggac cgtcgcaggc aacgcgaagg agctcgagct
    13981 cgccagcgct tcgctcagca gcgagcacga cgccatgaag ttcacgcgcg tgcctctcga
    14041 cggcgatacg ccggccgctc atgtgatgcg cacgggtgag cccgtgttcc tcgcgtcgct
    14101 gcacgagtat cgcgcgcaat ttccggaatc gttcgaacga ctgcgccagc tccgcgacgc
    14161 ctatcgtgcg atcgcgatcg tgccgatgca ggcgcacggc gtaacgctcg gcgtgatggc
    14221 gtactcgtac gcgaacgagc acacgttcga ggattcggag cggacgtttc agcgtctgct
    14281 cgcgaggcag tgtgcgctcg cgttcgatcg cgctcgcatg catcgcgaag agcggacgct
    14341 gcgcgaagcg gctgaacggc tcgcatccgc agagaagcaa gcgcgtggtg acgtcgagct
    14401 gctctacgag ctcattgcga gcgtcaatcg cctcgacggc atcgacgacg tgtatgccct
    14461 cgtgatgcgc agcgtgatgc gtggaacgcg ctcgcagcgc gcagcgatca tgcttttcga
    14521 cgagacgggg gcgatgcagt tcgaggcgag cgagcagctg tcgcgcgaat tcaaggctgc
    14581 cgtcgctggc cacacgccgt ggaagcgcga ggacttctat ccgctgccgc tggctgtcga
    14641 cgattgcgac accgatgcag agtgggccac attgcgagag gcgcatcgcg ccgaaggcat
    14701 ccgggcgctc gcagtcgtcc cgctgatcaa ccatcagggc cagctcctcg gtcagctcat
    14761 gctgtaccgc gacgatgcgc agccgtttac cggacgcgac ctgcagctcg cgtcgacggt
    14821 cggagtgcac gtcgcgcagg cggtcgagcg aacgcgcaag gagcgcgaga tctcacgcgc
    14881 ctatcgcgaa gagcgcgaag cgcggctgct cgcggacgaa gcgacgcgcg cacgcgaaga
    14941 gatcctgtcc gtcgtctcgc acgacctgcg caatccgctc ggcgcgatcc tcatgtgcgc
    15001 ctcttcgttg ctgcacgtca gcgccgacga cagcagtggc cgcatccgca caaacgtcga
    15061 gcgcatccat cgccaagccg aacgcatggc gcgctacatc agcgacctcg ttgatttcgc
    15121 cggcatcgaa gccggccgca ttcggctcga gcgtcgcgag catcaggtcg ccgagatcgt
    15181 gggcgcggca agcgagctgt tcggcccgat cgccgcggag cgtggcctca agctagagac
    15241 gagcatcttg cccgacctac cggcgatcga gtgcgactcc gagcgcgcag tacaggtgct
    15301 cacgaacctc gtcgcgaacg cgctcaaggt cacgccgaaa ggcggcgcgg tctcgatcgg
    15361 cgccgagacg aaggacaaca gcgtcgtgtt ctacgtgcgg gacacgggcc ccggcatcga
    15421 gcaagaggag ctgccgaacc tgttcgagcg gcactggcgt agcaagtcat cgaattacaa
    15481 aggcgcaggg ctcgggctct cgatcgcgcg cggcatcgtg agcgcccatg gcggcaagat
    15541 ctgggcggaa agccagctcg gggccggcgc cacgttctac ttctcgctga gccagctgcc
    15601 gacgaattag cggatttgcc gaaacaacgc ggttgacgtg gcctgggcgc cggatccaat
    15661 atcctggaca catgtccgcg atagcgcctt tggcttccac cgtcgttgct gtacctcgcc
    15721 gcctcgaggt ccgtcgcgat gcgatggtcg tcgaaggtca cgcgctcgaa gaggtcctgc
    15781 tcgcgggcta cgcgctcggg ccggagctcg gcaccgcgcc ggtcgtgctg atcgtcggcg
    15841 gcatcacggc gtcgccgttt ccattcggcg gtccagatgc agacgcgtgg tggccgtcgc
    15901 tcatcgcggc cgacttgatc gatccgacgc ggcacacggt gctcgccctg tgttggcccg
    15961 gcaacggctc gacgtggcgc ggtttcgacg acggggcgct gccgccgctc tctgcactcg
    16021 gcctcgcgga tctgatcgcg gcatggctcg acggtatcgg ctgcgcgcag cccgtcatct
    16081 tcgcgggcgc aagcctcggc ggtctggtcg gcgtcgcgct tgccgtgcgg catcccgagc
    16141 gcgtcgcgaa gctgctcacg atctcggccg gcttgcgccc cgatggttgg ggcacggcga
    16201 cgcgccacct gcagcgcgag ctcgttcgcg atgggctccg caccggtgac gcgacgactg
    16261 gcatgatccg cgcgcgccag ctcggcatgc tcacgtatcg cggtcgcgac gagctcgaca
    16321 cgcggttcgg cgtgctgtcg cccggtctcg agcagccgcc ggtcgccgcg tatctcgatc
    16381 atcacggcca gcgcttcgcc caacgcttcc cgctccgcac gttcctcctg ctcagtgaag
    16441 cgatcgatcg ctcgcgcttt gcctcggacg cggcaggcgt ccgtgatgcg ctcgcgcgtg
    16501 tcaccgcgga cgtgctcgtc gtcggagttc cgggcgactt gctgtttccg ttcccgctgc
    16561 agaacgagct ctatcgcgag ctgcaggccg ctggcgcgag tgcctcgctg tggaagctcg
    16621 actccgaata tggccacgac gcgttcctcg ccgatcaaga caagctcgcc gagctcatcc
    16681 gtgactcggg cttcctgcaa gcagcggagc cacggccgcg gttccacggc gtcggtgcaa
    16741 agccactgcg cgagatccgg atcggcatga tcggctgcgg caccgtcggc aacggcgtcc
    16801 tcgatctgct cgacaagcag aaggcgcagc tggccgagcg ctacagcgtg cgttatcgcg
    16861 tcacgcgaat cgcggtgcgc gatgcgacga agccgcgcgg gccgctcgcg aaggacatcc
    16921 cgctgacgac acgcgcgctc gatctcgtcg ccgatccaaa cgtcgacgtg atcgtcgagg
    16981 tggccggcgg cgatgcggtg gagcccgctc tcacggctgc tctcgccgcg ggaaagccgg
    17041 tcgtcaccgc gaacaaggcg ctgctcgcgc gccagctcgc gcggctcggc atgctcgcgc
    17101 aacgcacgga gacgccgctg ctctgcgaag cggctgcggc tgcagcgctg ccgatcattc
    17161 gtcacctgtc gcatcgcgct gacgaggtcg acgcgctgca agcgatcgtc aacggcacgt
    17221 gcaacttcgt gatcacccgg ctcgaacaag acgagctcac gctcgatcgc gcgatcgcgg
    17281 aagcgcagtc gctcggcctc gccgaagccg acccctccgc ggatatcgac ggccacgacg
    17341 ccgccgcaaa gctctcgatc ctcgcgtacc gcgcgttcgg cgcgtgggtt ccgcccgacg
    17401 ccctgcccgt tcgcggcatc ggcgagctgt ggcccgccga ctgcgacctt gccgaagcga
    17461 tgggctttcg cattcgtctg atcgcgcaag ctgcgcgggt gacgggcggc ctcaccgccg
    17521 cggtcgagcc ggtgctgctc cccgactggc acctgctcgc atctgtcgag gaggaataca
    17581 acgccgtgta catgaagacg gcgtcgagcg gcgacctctc gctgttcggg aaaggcgcag
    17641 gtgcgctgcc gactgcatcc gcggtcctcg gcgacttgat cgatctcgcg caagacaact
    17701 cggtccaatg gccaacgccg cgcgacatcg cgctcgtccc cgcgcctgct cgccgccact
    17761 acatccgcgt cagtgccgag ccgcatcccg gcctcacacg ccgcgtcgat tcgatcgttc
    17821 ggcgcgccgg cctcacggtg cagaaccgcg ccgcgcgcgg cgagccgctg atcacgcacc
    17881 atggcttcct catctcgccg tccgacgacg cgacaatccg cggcgtgatc gatcagcttc
    17941 gcgagctcgg tcgggtcgag caaacgctgt ggctcggagt cgtcgagtga cccgcagcaa
    18001 accccagcgc gcagacatgg gtgcggcgac gcgcgcgctg cacgacgccg tcgacgcgaa
    18061 cgcgccgtat ctgcatcccg agctccgcac gatccagcgc gacctgcgcg actcgtcaaa
    18121 cgttgtctcg tggcaggaag cgaaggcggc attcatcgtc acgggcgacg gcgcgtggtc
    18181 cgagctgccg cacctctacg ctcgctacgg aacgccgggc acgtcggagc tgatcgcaga
    18241 gctacgcaag ctcgagcacg cgcgctgcac gtttgtcacc gactgcggca tgcaagccgt
    18301 cgcactcgtc gccgacgtat cgatcgctca cggcgcgcac gtcatcgcga tgcgccaggt
    18361 ctacaacaag acgcgcgcgt tcttcgaagc gacgtgcaac cgcgtgggtg cgacgctcac
    18421 gctcgtcgac gatggcgacc tcaccgcgtt ggccgcggca cttcgcgccg aaacgcgctt
    18481 cgtgttcgcc gagacattca cgaatccgct cggtcgagcg caagacatcc cagcgatcgt
    18541 tgcgctagtg cgcagccgcg cgcccagcgc ccgcctcgtg atcgactcga cgatcgcgac
    18601 accgtggggc gtgcgcacac cgctgctcga ccaaggtgtc gacatcgtcg tcggcgcgat
    18661 gaccaaagca ctcggcggcc aagatgcggc gctcggcggc tacatcgcga cgaaccacgc
    18721 cgacgccggc aaccaaatca tggatctcat cgcgatgcgc ggcggcatcc tcgacgacga
    18781 acgggcgcgc cgcgtcgtcg cgcacctgcc gcaagccgag cgcaagcacg cgcgtcgctg
    18841 cgaaacggcc gcttccgtgg ccgccttcct cgcctctcac ccgcgcgtcg aagcggtgtt
    18901 ccatccatcg cgtcccgacc accccgacgc tgccgtcatc gcgcgcgact acacgcggac
    18961 gggctcgctg ctcgcgtttc gcgtgcgtga cctcgacgaa gcggcgcatg gccgtctcgc
    19021 cgatgtcctc gtgtcgtgcg gcatcctccg ctacgccctt tcgttcgatg gcctcgtcac
    19081 gaaggtcaac caccacaaga cggtctccga gtacttcacg ccggcggaaa acgtcgcgcg
    19141 cctcggcatc cagcgcctca tccgcctcgg catcggcctc gaagacgcgg aagacctcat
    19201 cgcgtgcctc gactggacgc tcgcgcaagc tgcctagtag cgcacgcggc atcgcctgcg
    19261 ttacataggg tggatgtcga ggcgggcatt ggtctggtgc ggatggcttg gtatcgcggc
    19321 cgcgataacc ggcgtcgtta gtggccttgg catgatcgcg tggcccgcgc acagcccacg
    19381 cgagctgctg cgatatccgt tttcgccggc cgagttccaa gccgtgcaaa cgtggttctt
    19441 cgtccaccac ctcgcgatgg tcgcggtgat gctcggcttc gcgcgctcgg gcgcgatggg
    19501 tacgggccgc atcatgcgag gtgcggcgtg ggtcgccgcg atcggcgtcg cgcttctcgc
    19561 agtgaacgag ctcaatgcga ttcgatatgc cgaatggacg ttgcacacgg cgaatgccgg
    19621 ggcgctcggc gcaggctatg gaatctcgac gaacctcgtc ggcctcggaa cgcttgtcgc
    19681 cggcatcggt gtgctgcgcg cgaagcgatg gactggttgg catcgttggg tcccgctcgc
    19741 gatcgcgctc gtgcatttcg tcggtgtcac gcctgcgctg ttcagcgaga gcttcgtcgg
    19801 cgcgcgcatc gcgatcgtca cgtgggtcgc cttgttcggc gcgctcggcg ccgcgctgat
    19861 ctccgagacg cgacgcgcgg ctgcgagcag caactagccg agcagcttac cgaggcgggg
    19921 attggcggtg cggcgccaga ggaatgcgtc gaggaagtag tgcgtcagct gtggcaccgc
    19981 gaggaggggc gcgatgatga cggcggcgtt gtcgatgtcg aagctgccgc cgaacagcca
    20041 ctcgcggtcg tgccacagcg agcggtccca gagcagctcc tcggcgtacg cgatgaacca
    20101 cagcgtcgcg aggaagatcg cgaggccgcg accgcgcgtg aagatgcgat ggttcgggcc
    20161 gtcgtgcgcg tccggatcgc gagcgccgtt gcgggcgtag atgtagacga gcgccatgta
    20221 cgggatgccg tggatgaaga cgttcgtgat cgtgaacgtg aagtccgtat tcgacgacac
    20281 gatgccgacg taccagcacg ccgccgtcgt cgcgacgacg aggtgcttgc cccacgagac
    20341 cgattcgcgc cgcacgatct gcatgcccgc acgcgcgaag tagatcacga gtaacagcgc
    20401 gtagacgagg ccggcgagat cggcgaccca cgtgggcagg ccaccgatga agtcgccctt
    20461 cttcatccat gcgaagtcgc gattcgcgag gtgcgcgtgc caccagatga gcggatagag
    20521 cgtcgcgagg tagaccgtcg cgccatcgag gaaacgaccg agcgcgtcgc gttcgccgtt
    20581 gcgtgcgcgg tagagcatca cccagccgta ttgctgtcgg atgaagtgga agacggcgag
    20641 atatgcgatc gcgcgccaga atggacctgg cccccacgcg tagagcgcga ctccgacacc
    20701 gaacatcgcg agcggcgtga gcgcgtagag catcgggcgg cggcgccact cgactggatc
    20761 gaaatagacg acgaacgagg tcgaccacac gtgcgccaca tcgacgagga gcacgccggc
    20821 gatccacgac cactcgggcg actcgcgacc aatcccgagc gatggagcga gcgcgacgag
    20881 cgcgagcgcg atgacggcgg ttccgccaaa cacggcgacg tcgatcggcg cgctgaacag
    20941 ccagcgcttg ctcggggagg ccgtcacggg tcgtccgaac gatactcgca tgacggctct
    21001 ttctgcccag cctcgacgct gatgtgcaca cgtccgtggg cacgtgtggg gctgcgccat
    21061 ccgtggaacg cctaagcgcg cgtgacgggg ccactccgaa cggtacgggg tttgctaaac
    21121 gcgcagccat gtatttgctt cgctttggcc ttcttgcggc gctggccgca accgcctgcc
    21181 acgagtcagc aggtggcgac gatgacgcta cgccactcga gtgtcccgcc gagactgcaa
    21241 cgacgggcgc gctcgcgctc gacggcgtcg atgattacgc gcgaacgccg aaggtcgtcc
    21301 ccgcgctcgg gctctcgacg ttcacgatcg aaacgtgggt gcgtcgcgat ggcgagggcg
    21361 tcacgtacac gactggtgca ggcggcttgc gcctcgtccc gatcgccggc aagggactcg
    21421 gcgaaggtga caagagcaac gtggactgca actacagcgt tggattttgg ggcgacgtgc
    21481 tcggcgccga cttcgaggat atggcgacgg gcgcgaacca tcccgtgatg ggcaagaccg
    21541 ccgttccgcg cggcgagtgg catcacatcg cggcaacgta cgacggcgca gcgtggcggc
    21601 tgtacctcga tggcaagctc gacgccgagg ctgtcgcgaa tgcgacgccg cgggccgata
    21661 gcattcatcc gttcggcatc gggaccgcga tcgacagcgc gggtgcgccg catggcttcc
    21721 tcgcgggtgc gctcgacgag ctgcgagtgt gggatcgcgc gcgcacgccc gaggagatcg
    21781 cggacggcat gtaccgcagc atcgccatgg ccgacggcct cgtcgcgcgg tgggcgttcg
    21841 atgcgggtgg cgtcgaccct gcggtcgccg atgacagcgt gcacacggcg ccggtcgcgc
    21901 tcaccggtgg tgcggaagtc tccgtcgccg acgtcgccgc gctggatcac ggtagtgcgc
    21961 cagtcgtcgc agcggtgagc ccggcggacg gtgctgcgag cgcgcccggc gtcgtgctcg
    22021 acgtgtctct cgagatggag agccgcgctc ccgtcgacgt gacgtatcac gtgcgcgagc
    22081 tcaccgacgc ggacgacttc acgatcgtcg tcctccccga tacgcagatc tacacgctcg
    22141 agggacgcaa cctcgagaag tacttccacg accagaccca atgggtgcgc gacaatcgcg
    22201 agcgctacaa catcgtcggc gtcatccaca acggtgacgt catcaacaac gagccgcaga
    22261 tctatcagtg ggaagtcgcg agcgccgcgc ttgcgcgcct cgagaaacca gagcccgccc
    22321 tgcccgacgg catgccgtgg ggcgtcagcg tcggcaacca cgacaacaag ctgatcggcg
    22381 cgaacacggt tctcaacacg gacaagttca accagtactt cggcgtcaac cgcttcgcgg
    22441 gacgcgcgta ctacggtggc cactacggga cgaagaacga tcacagctgg gtcacgttct
    22501 cggccggcgg actcgacttc gtcgtcgtga gcctcatgta cgagctcgat ccggatccgg
    22561 ccgcgatcgc gtgggcgcgc agcatcttcc agatgcaccc gaacgcgttc ggcatcctca
    22621 acacgcacta ccttctgacg gcgagcggtc gattcagccc gcagagccag cagatctatg
    22681 cggcgctgaa agatctgccg aacgtgcagc tcatgacggg tggtcacgtc gcggccgaga
    22741 gccggcgcgt cgacgcgttc gagggacaca cgatcagctc gatgctcgct gactttcagg
    22801 gccgcgacct cggcggtggc ggcttcatgc ggatctggga gttctcgccc gcaagccaga
    22861 ggctgagcgt gcggacgtac tcgccgacca tggacaagtt cgagacagac gccgacagcg
    22921 agttcacgct cgacgtcgac ctgcgaggtg ctggtggtcc gttccgaacg ctgcaggtga
    22981 aggcggcgga tcctgcgcac gtcgccctgc ccgtcgacgg tctcgaggtc ggtaagagct
    23041 atgagtggta cgcggaggtc gcgagttgcg gccaccgcat ggcgacgccg ctctatcgat
    23101 tcacgacgat ggcgtcagct cgcgccgcgc agcagttcaa cgacacgctg tatcagccaa
    23161 gcggccgcga ccgcaccaag cgcgtaacaa gcggcgtcgt gaacgcgcag ccggacgatc
    23221 catcgctcgt ggactagccg ccgcccgatc gcgacgacga gcagagcacc cgcgaccacg
    23281 gcgagctgcc ctgcttcgac gccgatattg aacaagacga ggctgcgcgc gagcgatccg
    23341 ctgccgagcc cgagctcgcg cagcgccgac gcgaagccaa ggccgtgcac gaggccgaac
    23401 acgcccgcga cgatccacgg cctgcgacgg atcagcgtcg cattgtcatg cagcgcctcg
    23461 cgtgcgacga gcagaacgct gagcgcgatg cacgcctcga cgggtgcggc gttgacctga
    23521 atcacgccgg caacagcgag tgcgagcgtc agcgaatgag ccacggtgaa tgccgtgatc
    23581 gtcgcgagca ggcggcggtc gacggtcatc cggagaacga gcaagaggcc gagcacgaat
    23641 gcgaggtggt cgaagccggc gaggatgtgc gtgataccga gccagatcca cgcgcgcagc
    23701 gaggacggcg ccgcatcatc gatcgagatc cgtgggtgcg ccggatcgac gatccattcg
    23761 cgacgctcgc ccgagcgcac gaccacgacg acggtcttca tcggccccga catgccgatg
    23821 acaccgagct gtccggcaag gccgctcgcg cagcggacgc gaacgcccgt cgatgcacag
    23881 cccggcggaa gctcgatgcg gacgtcgacg gcgtcgcctc ggctgtcgat cggcggcacg
    23941 aaatgaagcg cgaagtcgcc gtcggaaagc tcggtcagcg acagcacgcc cgggcgtaag
    24001 tcatgcgcct cggcgacgct cgcgctcgcg accaaggcaa gcaccacaca cgcgcgtctc
    24061 atcgctgctc gaccgtccac gtcgcgcgca gctgcttcat cgcattgcga acgagctctt
    24121 ccttcttggc gtcgagccac gccttgcgaa cgtcgagctt ggcagcagcg aagtcgatat
    24181 cgcgcggtgc atcgacgcgg tcgacgcgca ccaggtggac tccatgacgc gacgtgcgct
    24241 tgacccacgc accgggcgat tgactcgcga ggccatcgac aaactcggcg ccgaacgact
    24301 gcgcgaggtc cgcgagcttg cgacctcgaa acttgtgccc tgccgggaac cgatcaccga
    24361 gccgctcggg tggcgcgcca ttcgcaagtt gtgtcagcag ctcgtcggcg cgggcggtcg
    24421 cattgtcgcc ggtaacgaag acatgggtga agtcgacatg ctcgggcacg gaccactgct
    24481 cgcgatgcga gtcgaaccat gcacgcagct ccgcgtcggt cggctcgggc actacgagcg
    24541 cttgctcgag cacgtagctc atgcgtcctg cgatgcgctg atgaatcacc gggtcgtcgc
    24601 gatcgagccc gcggctgacg gcctcgcgat acaggatctc ctcgtcgatc caacgctccg
    24661 tctcgtccgc gatctccgca ggggttggcg cgcggccaag ccgccgctct gccgcctccg
    24721 cgctcgcagc gcgcactgcg ggatcgacca cgatgcgccg ctccgcgaca cccgcatccg
    24781 cgatgggcgt tgctgacgag ggcgtggacg agctcggagg cggcgacgtc cgcggtgttg
    24841 gctccgatct ccacgcatcg agcgcgaaca gcgcggcccc gatcgcgagg aagtgcacga
    24901 gcggctcgcg cgcgatccgg gacactcgcg gcatgctcgt gaagtatatg cgcggctcta
    24961 gtccgggtag catgcgcgca tgaagttctt gacgtgcgcg ctcctctgca tgctcgcatg
    25021 cggatcgccg caacccgccc ccgtcgcgcc gccactcagt ggctccgccg ctgcgcccga
    25081 agcgccgcct gtcgcaacgc ccgtcgccat cgcatttgtg atcgaaggct gggagatgtg
    25141 ggtcggcaac gatcagattc ccgacatcga cgagcgcgag cagtaccaag gtgctctgca
    25201 gccgttcaag gaggcgttcg gccggctgaa actgacagac tttccggcag gctcgcaggc
    25261 ggcagtcgtt acgtacaccg atcgcgcgac ggtgcggcag ccgatgcagc cgatcgacaa
    25321 gctcacgccc gcggcgtttg gcgagcagaa ggactacttc ggcacgatcg atcgcgacct
    25381 cgtcagcggt gtaaccgcgg gcctcgacga gctcgccaag gcgaagaacg cgcgtcgcat
    25441 tcttgtcgtc atcggcgacg gcaatgacag ccacccgagc acggcgaagg cggcgctcgg
    25501 tgagctcgcc aagcgggctg cggcagagaa ggtcgaggtc gtgtcgctcc gttacttagg
    25561 gccgctctcc tcgccgagct cgaacgtcag cgcattcgat ccggccacgt tgacgattaa
    25621 ttcgatcgcg ggcatcacgg accagctcga cttcctgttc acgcgactga agaagccacc
    25681 cgtcgctgcc gcctcgacga ctgcgatcaa agcgccgctc gtcgtggcct tcctcgtcaa
    25741 cggcagcgag gtgtggatgg gcaacgacga tgtcgagcca caggattccc ccgccaggta
    25801 tgtcggcgcg ctcaagccga tgcgcgcggc gttcgagaaa gctgcgctga ccggatttcc
    25861 agctggctcc gtggcgatgt tgatcacgta cgattcggcg acgaaggtgc gagtgccgcc
    25921 gatggcgatc gagaatttcg gtgcccgcgc gctcggcgct caaggcgatt accacggtgg
    25981 attcggcacc gagcttgtca gcggcgtaac gggcgcgttc acggagctcc tgaagatcga
    26041 cgcggcgcgg cgcgtggtcg tcgtcatcgg cgacggcaac gatacgaaca acgacaccgc
    26101 gaagacgcag ctccaggggc tcgcccttcg cgccgccgaa tatcacatcg aggttcacgc
    26161 cctcgtcttc aaggggcagc tatcggagcc gggcaccgtc gtgacggcgc tcgtcccgaa
    26221 tgccgaaacg gccgcatcgg cggacgatct cacggcgaag ctcgtcacgc tgttctccac
    26281 actgcgaaag aagtagcgcc atgagaagac tcttcgtcat cagcctgctc gtcgtcggct
    26341 ccatcgcgta cgcggacaaa ccaaaggctc ggaagccggt gaggcccgtg ccgcagaatg
    26401 cgatcgtggg gcgcatcgtc gggctcgaca tctacaccga cgaaggccgc gacagctcgg
    26461 tcatcaccgt cgccgcggga tcggagaacg gtattagcaa gggctggcac gcgcggtttc
    26521 gcgaaggcac gacgagcaac cgcctcagcg gaggagacgc caccgtcatc cgcatcgatc
    26581 gccgcaccgt cgtgctcaaa acggaccttc ccgccgagca ggtgcgtgcg aaccgctaca
    26641 tcgaattctc accgcgttag gcgcgtagac cttcgacgac ttcgcgcgcg gcacgcaggc
    26701 cgtgatcgaa cgcctcttcg aagagcgcga tgcccgagag gtctgtatgc gcgaagtgca
    26761 cacggccgat cggtgtgcgg tcacctcccc gcgcgacgag cgactcgagc gattgcttcc
    26821 agatcgaacc gacgcgcggt cggatcatgc cgtggcccca gaacgcgacc tcgatatgct
    26881 cgacatggtc gcgaacatct ggatgcgcgc gcgagatatc ggcgaggatc gtttcggccc
    26941 actctgcgta gccagcgccc gcgagcttct tgcggaccga cgtcgaatct tcgtcggtaa
    27001 acgggtagta ccaggtccac actgtcggcc ctcgatctcg accgcgttga tgcgtcgccg
    27061 agacgtagcc gagtgacggg ctatcgcgaa acacgttgtc ccacgcgggc ggcgcacccc
    27121 ggccgcgctc gatcgggcgt ccacgcaggt gaacgtttgc gacgacccac gcgccatagt
    27181 cgggccctga ccgatgcgcc acgggcggcg gcacgtagtg aggcgtgatc ggtcgtcgac
    27241 cgcgcacgat ccggttcgcg acgaacgatg gggtcgcgac gatgatcttg tcggcttcga
    27301 ttccgagcgg acccgacgga ccgacggcca cgatgcggcc tgtcacctcg tcgatgtcga
    27361 cgaccgcgat gccacgctcg atcgtcgcgc ccaccgcgag gtgcgtgatc agcgcgttgt
    27421 tcccatcggg ccacgtgacc accgactgcg cttcggcacc cggcgcggac tggcgtccgg
    27481 cgaagtagaa caggcccgcc catgcgctcg tttgcgcggc ggtcaatgcg tagtcgtcgc
    27541 ggcacgcgta gtcgcacagc cagcgcaagc gctccgacgt caggccgcgc gcgtcga
//
LOCUS       DATLBZ010000207        15068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7552824, whole genome shotgun sequence.
ACCESSION   DATLBZ010000207 DATLBZ010000000
VERSION     DATLBZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15068
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7552824"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2938)
                     /locus_tag="VMZ53_10915"
     CDS             complement(<1..2938)
                     /locus_tag="VMZ53_10915"
                     /inference="COORDINATES: protein motif:HMM:NF020928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HUS29017.1"
                     /translation="MSEDAPSSTPAKKLDFDPVPALAQSLGLPPKSIAAVIALLDEDN
                     TVPFIARYRKERTGGLDEVQIREIETQRGYLVELEERRAAILASVGEQGKLTPELEQK
                     LRAATGKAELEDLYAPYRPRRKTRASVAREKGLEPLAQKILAQASDGDPQGDAAAFVT
                     EQVASVDDALAGARDIVAETIADTAEVRAYVRKEYAEQGKLVSVVVPGKDAEPTKFEQ
                     YYAFEEQVKAIPSHRFLAIRRGEAEGVLRAHVSVDIAKVEGGILNLVKHAPASPWGQQ
                     LEQAVTDALKRLLAPSVENDVRAELKSRSDSAAVEIFAGNLRNLLLAAPLGSASVVGV
                     DPGLRTGCKCAAIDATGKFLGTVTVYISQGDAQLAKAKDDFIAFVQQQKPRAIAVGNG
                     TGGREAEAFVKKCLADAGLREGPDAPFVVQVNEAGASVYSASDLAREEFPELDLTIRG
                     AISIARRLQDPLAELVKIEPKSIGVGQYQHDVHQTLLGKKLGDVVESCVNHVGVELNT
                     ASAQLLGYVAGIGKSLAKKIVVYRDTNGAFASRQQLQDVSGLGPKAFEQAAGFLRIHG
                     AQNVLDASAVHPERYTLVEQMAKDLGVELPQLIGNQELVDKIDLSKYVADDVGEPTLR
                     DITAELGKPGRDPRAEFAPPKFRDDVTTMEDLKPGMVLEGVVTNVTAFGAFVDIGVHN
                     DGLVHVSQLAEKFVKDPSEVVKVGQKLTVRVLEVDNQRKRIALSARPPARERQQQNQQ
                     RGPGQQQGGGGRGGRGRGGGGGRDQQQWQGGGQAGQPGGGDQHADAQGGGPRGDGRGQ
                     GGGGRGGQGGGRGPRGGGGGGGGQGQGQGGQGRRNDRGPRRDDQRAQGGEGMPANGDI
                     AANVGPNVDRAGMTLDGQFPGGGGNAGGGDSAGGDESRGNHASGEFRGGGGGGGGEFR
                     GGGGGEFRGGGNRGGGRRDDRAGGGGGGGSGGGGKRGGGGGGAGGGGGGGGGNGGNRS
                     GRGPRPD"
     gene            complement(2989..3786)
                     /locus_tag="VMZ53_10920"
     CDS             complement(2989..3786)
                     /locus_tag="VMZ53_10920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS29018.1"
                     /translation="MTSIARFVGPIPELYDRHMGPVLFEPYAEDIAARLPKTAKRVLE
                     IAAGTGRVTRHLLAALPPDGQLVATDLNEPMLVRAQTVISDPRVTWQTADMLALPYPD
                     GSFDAVVCQFGLMFVPDKVQALREMKRVLVAGGTLLLSVWDSLAHNPASQQLHHLAYG
                     LMADNPPTFMATPFSMHDTAALRVMADEAGFRAVNIETVATTGTAESAKHLATGFVRG
                     NPLWLQLLERNLDGDAFEAQIAAALATEFGESPCKTPLSAHVLTATA"
     gene            3819..4841
                     /locus_tag="VMZ53_10925"
     CDS             3819..4841
                     /locus_tag="VMZ53_10925"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HUS29019.1"
                     /translation="MPLDPKRESLIRRLPKVELHLHIEGTLEPEMMFSLAAKHGAKLP
                     YASVEAVRAAYNFHDLQSFLDLYYAGCDVLRDRRDFYALAMAYFTRAHADNVVHAELF
                     FDPQTHTVRGIPMDVIIGGLRDAMVDAEARYGISSQLILCFLRHLSEEEAFKTLADAL
                     PYKDQIAAVGLDSGERGNPPSKFERVFKYAKELGLRTVAHAGEEGPAAYIREALDLLF
                     VERIDHGVRCDEDPALVEELIKKQIPLTVCPLSNLKLCVVKDLREHNFAKLLRKGVAV
                     TINSDDPAYFGGYIGENYRATADALALSEQEIITCAENAVHASFLPNAQKLSLTQAIQ
                     LEARRG"
     gene            4838..6460
                     /locus_tag="VMZ53_10930"
     CDS             4838..6460
                     /locus_tag="VMZ53_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29020.1"
                     /translation="MTLDADAAKIPSLIEHVAAPKVASRWEELAQLGDLACGGDHEPY
                     IFTGYSGGGESHTAVAAGHATYLKVLAGKDAKARAAAAFVLAWLPEHAKKSLAALHKV
                     IKKEKDATARASMILALAVLAPGADLKLDTEALGSKEAPVSGAGLVAAAIAGQLPAKA
                     TFVAPTLARADTIGALCWQHGDLGTLVMRAVLHASSRDNDATVLTNMIFAERGYLLLP
                     DVIKLLFPSRIEAAKLTKPQRELLDKLTLDPTRYGYAQLPKLWEERGLPTDAEAARAQ
                     LGLTEVEEPLEEIIAVGRGKKTFAEHLAEAVADPERRKPLAVALAKENSAREVVELCV
                     EGCTQEEGPDFQDTVVELFEACGPDLVPALRELDIYKEVQLDDGRAIQPTQLAAPALL
                     LLARRDPALPDDKLDQLWAAASARHLIEPILAALPPDRRARKLADAYRNVPSWEIGEK
                     TRLEKLMLAAPAPELTAELLAHVKELKDAYANGRKMEGTLFSEGYSPAKLKKLKPRAH
                     PLTPKLIENIEAYAHASDDKQLGKAIAQFLDM"
     gene            6478..7392
                     /locus_tag="VMZ53_10935"
     CDS             6478..7392
                     /locus_tag="VMZ53_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29021.1"
                     /translation="MRNRLFVLAIVLCADVAHADEVDDLVAKGQELAKQSEFTQAIAT
                     FKQADAKRPRAAHACMIGLAYMRREAWPQAELFLALCEKRAAPGDQPPEWIDEAKKQL
                     AQKLTAAQIPAVTIDVTPPNIGAHLTVSSFAPDEVFDPQTIHLAPGTHVIEVTAPGYR
                     SERKDVVVEPAKPQHVTFEMQRAGDAVAPVSPRQPERGSHSKVPWLVGGAGVAIGVAG
                     IVYHATSVSAAYDRADGAETMAAYNVLVDDFNSKRNVAVGLYAASSLTIGVAVVLRFT
                     VYKDASEPPLVTAQLGDHGAGILIGWQR"
     gene            7389..8408
                     /locus_tag="VMZ53_10940"
     CDS             7389..8408
                     /locus_tag="VMZ53_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29022.1"
                     /translation="MKQLALLALLGGCLTPPAAPGGPERAWTRVADGALPGRMHGARL
                     TWDPQRDAIVMYTGYNATQNLAPGYVYKWQANEWMRICEEGVGPPPLYLPGFTWEPSG
                     QLVLVGGAQIVPGAGLFDNVSKDIYTCDTTGVWTKQTNTLQRERAGASLIYDPQRDRL
                     MLVGGRDRNGPIKNAEMSAPNAQDFTIHPAQMPFASAGAGQTAMYDADSKMIFALEGE
                     TTGDDDTPLHDAIWTYDGAAWTRFCNDCSGAPRADASIVHIHGTNETYVIAGFAGNSR
                     DHAGTWVIDQNKLLRVFDEPDARASVGAAYSDTTDSIVMFGGTSVNCEEYECDATFEL
                     SIQPQ"
     gene            8405..8950
                     /locus_tag="VMZ53_10945"
     CDS             8405..8950
                     /locus_tag="VMZ53_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29023.1"
                     /translation="MTPRTLAQLEAATAHVRAAPREEGIVTFMVRRPDKGAREILDEA
                     QLDTRAGMIGDRWVNSTSKRTPDGQPHPEQMLTLMNVRAIDAIAERSYWPLAGDQFYV
                     DFDLSKASLPPGTTLAIGNALVVITPIPHTGCAKFTERFGSDATKWVNSDEGLELRLR
                     GVNARVMRGGVVRVGDSLRRT"
     gene            complement(8951..10489)
                     /locus_tag="VMZ53_10950"
     CDS             complement(8951..10489)
                     /locus_tag="VMZ53_10950"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HUS29024.1"
                     /translation="MLLAELVATSAQVTETSGRLKKIELLAKCLRQMEAEERAIGARY
                     LAGELPQKTNIGYATVAEVMRQAMPAVAAYLALGDVDRRFTAIAELRGTGSANARRDH
                     FGQLLSLATAAEQKFLGGLAVGEVRQGALDGLVVEAIAKAAELPADSVRRAYMLAGDL
                     GEVAAAALEGGAAEIGRFGLTLFRPVLPMLAQTADDAADALTTFGGPASLEYKLDGFR
                     VQIHKDGEIVKAYSRALNEVTDYVPEAIAAIAKLPAQKLIVDGEAIVFGANGRPLPFQ
                     DTMKRFGKNGPLLADKMPLSLALFDTLLIDGQTLMTEPAKRRLDELDALAPAHSVKRL
                     ITSDPQEASAFYAQAIASGHEGVMAKALDAPYDAGNRGASWLKIKKVHRLDLVVLAAE
                     WGSGRRKGWLSNIHLGARSGDDYVMLGKTFKGMTDEMLKWQTEKFLSLEVDRDGHIVY
                     VKPEVVAEIAFNDVQRSPRYPAGLALRLARVVRYRDDKTAVQADTIDAVRAIAIADGV
                     LAAS"
     gene            complement(10504..11907)
                     /locus_tag="VMZ53_10955"
     CDS             complement(10504..11907)
                     /locus_tag="VMZ53_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HUS29025.1"
                     /translation="MRKVLLFSLPLLIGACDMQDDGPTPYDDDYGVTPGNINEGAPNN
                     DSLPDDNKADAVYPAKFELPKNEQSAVKSQGSRGVCSIFAATAIVENLYLKAGMPAAE
                     VDFSEQYMQWSVKNQVGAFQHTEGSNVDDNLQAVVEFGDIKESDWAYESAPWTAANDP
                     ECTGGESLPTKCYTNGEPPQTALDAPKFKLPSSRWINTNSIKAHLTTKKTGVGVGLNF
                     FYQSWNHRRSTIPVDSELWRQGFVTYPNDKDKTESATHRAGHAIEIIGWDDDLEIPMR
                     DGEGKPILDANGQPKKEKGFFLFKNSWGTAGFGIDHPYGAGYGWLSMRYVKEYGSAVV
                     GEVPVLNGPTPPPPSGGQTHSYSAMPASAIPDNAPAGVSSTIEVADAGTLASVKLTTD
                     VTHTYRGDLKVTLTHGATTVTVFNGEGGSADDLKQTFTVSGFSGELKGTWTLKVVDTA
                     SQDVGTLNSWKLEVATN"
     gene            complement(12025..13866)
                     /locus_tag="VMZ53_10960"
     CDS             complement(12025..13866)
                     /locus_tag="VMZ53_10960"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HUS29026.1"
                     /translation="MKIVCDACQAKYSISDDKVQGKVFKIRCKKCSNIIVVRGGAGAA
                     EAAPAPAEKDTRVYDYGYEGGAGGGGGGDDAVWHLVINQDQVGPLSVAEVQSKFASGE
                     VDGETFTWREGFADWLPLAQVDQFSAMVASGSTTTAGSGGGAAAVAGMFGSGGIGTDD
                     SGTSRSDPGDLFAAASSSSGSSRSSRDEDDGGDLFGSKPSASSSSSAAASASSQKLRG
                     ERNENSVLFSLNNLAQLASDTPRAAAPSSSSGSSGSSSSGHATGAAGGEGSGLIDIRS
                     MASAYLGGSGGGAAVGKPASASSGIGSIDDLPVFGGGGFSEPAVIVPTARPAANNKML
                     MLMIGAVGLLAVVAIIMVVLLLKGGKQNPQVASATPKTDTEEKKDETKDDKKDEVKTP
                     EAGSATETKTDTGSTAGSATETKTTETKTPETKTTETKTTETKHTTPKTGGGGSTTTP
                     KTGGGGTTTPKTGGGGSTTTTAATTTKTETGGGGGCDEVSCVLNNYEGACCAKFKKGG
                     APKTGGGGGGGGGGGKSDLPEGLDRSMISSGIANVKARVQACGDKSPAKGKVKVKVSV
                     GGDGRVSNVAVETTPDSALGACVAAAVQKASFGKTQNGGSFSYPFVF"
     gene            complement(13958..>15068)
                     /locus_tag="VMZ53_10965"
     CDS             complement(13958..>15068)
                     /locus_tag="VMZ53_10965"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HUS29027.1"
                     /translation="CVLGDGMVIDPHTLLEEIAECQERGLLMRNELLIGLGAHVILPY
                     HKLIDGLREDHGAKSGKAIGTTRRGIGPAYEMKAARRGIRMRDLLKPERLHNLVSANL
                     DDLAPLIQHLGGAVPPKTEIDQWINDALAAGEKLRKYTGDAGRFVANVIADHKHVLFE
                     GAQGCLLDLDHGTYPYVTSSSTIAAGACQSAGIGPTQIDHVVGITKAYATRVGEGPFP
                     TELHGEMGERLRKAGGEYGATTGRPRRCGWLDLPALRMGVRLSGMQSLALTKLDVLAG
                     LGEVSVCVAYKLDGKELDEPPTDPEDLARAEAVFQRFPGWDKLPADAHSIEDLPASAR
                     SYVQTISQMAGVPFCLVSVGPDRAETIRLMDPFAN"
BASE COUNT         2488 a         5067 c         4901 g         2612 t
ORIGIN      
        1 catcaggacg cggaccacgg ccgctacgat taccaccgtt gccaccgcca ccaccgccac
       61 caccgccggc gccaccaccg ccaccaccgc gtttgccacc gccaccggac ccaccgccac
      121 cgccaccgcc agcgcgatca tcgcgacgac caccgccgcg attgccaccg ccgcggaatt
      181 cgccgccgcc accgccgcgg aattcgccgc cgccaccgcc gccaccgccg cggaattcgc
      241 cgctcgcatg gttgccgcgc gattcgtcgc cgccagcgga gtcgccgcca ccggcgttgc
      301 cgccaccgcc cgggaattgg ccgtcgaggg tcatgccggc gcggtctaca ttcgggccga
      361 cgttcgcggc gatgtcaccg tttgcaggca tgccctcgcc gccttgcgcg cgttgatcat
      421 cgcgacgcgg gccgcgatcg ttgcgacgac cttggccgcc ttggccttgg ccttggccac
      481 caccgccacc gccaccgcca cgcggaccac gaccaccgcc ttgtccgccg cgtccaccgc
      541 caccttgacc gcggccatcg ccgcgcggac cgccgccctg agcatcggcg tgctgatcgc
      601 cgccgccggg ctggccagct tggccgccgc cttgccactg ctgctgatcg cgaccgccgc
      661 caccaccgcg accacgtcca ccgcgaccac cgccgccttg ttgttggccg ggaccgcgtt
      721 gctggttttg ctgttgccgc tcgcgagcgg gcgggcgcgc ggagagcgcg atgcgcttgc
      781 gctggttgtc gacttcgagg acgcgcaccg tcagcttctg gccgactttc acgacctcgc
      841 tcggatcctt gacgaacttc tcggcgagct gcgagacgtg gacgaggccg tcgttgtgga
      901 cgccgatgtc gacgaacgcg ccgaaggcgg tgacgttcgt gacgacacct tcgagcacca
      961 tgcccggctt gaggtcctcc atcgtggtga cgtcgtcgcg gaatttcggc ggcgcgaatt
     1021 cggcgcgcgg atcgcgaccc ggcttaccga gctcggcggt gatgtcgcgc agcgtcggtt
     1081 cgcccacgtc gtcggcgacg tacttggaga gatcgatctt gtcgacgagc tcttggttgc
     1141 cgatcagctg cggcagctcg acgcccaggt ctttcgccat ttgctcgacg agcgtgtagc
     1201 gctcggggtg gacggcggat gcgtcgagga cgttttgcgc tccgtggatg cggaggaagc
     1261 cggcggcctg ctcgaacgct ttcgggccga ggcccgagac gtcttggagc tgttggcgcg
     1321 aggcgaaggc gccgttggtg tcgcggtaga cgacgatctt ttttgcgagc gacttgccga
     1381 tgcctgcgac gtagccgagg agctgcgcgc tcgcggtgtt gagctcgacg ccgacgtggt
     1441 tgacgcagct ctcgacgaca tcgccgagct tcttgccgag gagggtttgg tggacgtcgt
     1501 gctggtactg accgacgcca atcgatttcg gctcgatctt gacgagctcg gcgagcggat
     1561 cttggagtcg gcgagcgatc gagatggcgc cgcgaatggt gagatcgagc tcggggaatt
     1621 cttcgcgtgc gaggtcggag gcgctgtaga ccgaggcgcc tgcttcgttg acttgcacga
     1681 cgaacggcgc gtcggggcct tcgcgcaggc ccgcgtcagc gaggcatttc ttgacgaacg
     1741 cttcggcttc gcggccgccc gtgccgttac cgacggcgat cgcgcgcggc ttttgctgct
     1801 gcacgaatgc gatgaagtcg tctttggctt tggcgagctg cgcgtcgcct tgagagatgt
     1861 agacggtgac cgtgccgagg aacttgccgg tcgcgtcgat cgcggcgcac ttgcagccgg
     1921 tgcgaaggcc cgggtcgacg ccgacgacgg atgcggagcc cagcggcgcg gcgaggagga
     1981 gattgcgcag gttgccggcg aagatttcga cggcagccga atcgctgcgc gacttgagct
     2041 cggcgcggac gtcgttctcg acgctcggtg cgaggaggcg cttgagcgcg tcggtgaccg
     2101 cttgctcgag ctgctgcccc cacggggatg cgggcgcgtg cttcacgagg ttgaggatgc
     2161 cgccttcgac ctttgcgatg tcgacggaga catgcgcgcg gaggacacct tcggcctcgc
     2221 cgcgacggat cgcgaggaag cggtgcgacg ggatggcctt cacctgctct tcgaacgcgt
     2281 agtactgctc gaacttcgtc ggctccgcgt ccttgccggg aacgacgacg gatacgagct
     2341 tgccctgctc ggcgtactcc ttgcggacgt acgcgcgaac ttcggcggtg tctgcgatcg
     2401 tttcggcgac gatgtcgcgc gcgcctgcga gagcgtcgtc gacggatgcg acttgctcgg
     2461 tgacgaacgc cgcggcatcg ccctgcggat cgccgtcgga tgcttgcgcg aggatctttt
     2521 gcgcgagcgg ctcgaggcct ttctcgcgag cgacggatgc gcgcgtcttg cggcgcgggc
     2581 gatacggcgc gtagagatct tcgagctcgg ctttgcccgt tgcggcgcgc agcttttgct
     2641 cgagctccgg cgtcagcttg ccctgctcgc cgaccgacgc gaggatcgca gcgcgtcgct
     2701 cttcgagctc gacgaggtag ccgcgctgcg tctcgatctc gcggatctgg acttcgtcga
     2761 gaccgcccgt gcgctccttg cggtagcgag cgatgaacgg gacggtgttg tcctcatcga
     2821 gaagcgcgat caccgccgcg atgcttttcg gcggcagtcc caacgactgc gcgagcgcag
     2881 gcacgggatc gaagtcgagc ttcttggcgg gagtagacga aggtgcgtcc tcgctcattt
     2941 gggcccgcga acctagccgt gtcgccctgc cccggcaagc gggaaaggct acgcggtggc
     3001 cgtcagaacg tgcgcggaga gcggcgtttt acagggagat tcgccaaatt ccgtggcgag
     3061 cgcggccgcg atctgggctt cgaaggcgtc gccgtcgagg ttgcgctcga gcagctgcag
     3121 ccaaagcgga ttgccgcgca cgaatccggt cgcgagatgt ttcgccgatt ccgcggtgcc
     3181 ggtcgtcgca acggtctcga tattcaccgc gcgaaatccc gcctcgtcgg ccatcacgcg
     3241 caatgcagcc gtgtcgtgca tcgagaacgg cgttgccatg aacgtgggtg ggttgtcggc
     3301 catgagcccg tacgcgagat gatgcagctg ttgtgacgca gggttgtgcg cgagcgagtc
     3361 ccataccgac agcaagagag tcccgcccgc cacgagcacg cgcttcatct cgcgcagcgc
     3421 ctgcaccttg tccggaacga acatcaggcc gaattgacag acgaccgcgt cgaacgagcc
     3481 atccggatac ggcaacgcaa gcatgtcggc ggtctgccac gtcacgcgcg gatcgctgat
     3541 gaccgtctgt gcacgaacga gcatcggctc gttgaggtcg gtagcgacga gctggccgtc
     3601 gggcggcagc gcggcgagga gatgacgcgt gacgcgcccc gtgccagcgg cgatctcgag
     3661 cacgcgcttc gcggtcttcg ggagccgcgc ggcgatgtcc tcggcatagg gctcgaagag
     3721 caccggcccc atatgacggt cgtacagctc gggaatcggc cccacgaacc tggcaatcga
     3781 cgtcatcgac ggctcttatc gcatatgttc cctaccccat gccgttggat ccgaagcgcg
     3841 agagcctgat tcgccgtttg ccgaaggtcg agctccacct ccacatcgaa ggcaccctcg
     3901 agcccgagat gatgttcagc ctcgcggcca agcacggcgc gaagctgccg tacgcgtcgg
     3961 tcgaggcggt ccgcgccgcc tacaacttcc acgatctgca gtcgttcctc gatctctatt
     4021 acgcgggctg cgacgtcctg cgcgatcgcc gcgacttcta cgcgctcgcg atggcgtact
     4081 tcacgcgcgc gcatgcggac aacgtcgtcc acgccgagct gttcttcgat ccgcagacgc
     4141 acaccgttcg cgggatcccg atggacgtca tcatcggcgg cctgcgcgac gccatggtcg
     4201 acgccgaagc gcgctacggc atcagctcgc agctcatcct ctgcttcctg cgccacctct
     4261 ccgaggagga ggcgttcaag acgctcgctg acgcgctgcc gtacaaagat caaattgcgg
     4321 ccgtcggcct cgactcgggc gaacgcggta acccaccgag caagttcgag cgcgtgttca
     4381 agtacgccaa ggagctggga ctgcgcacgg tcgcgcacgc gggcgaagaa ggaccggcgg
     4441 cgtacatccg cgaggcgttg gatctcctct tcgtagagcg catcgatcac ggcgtgcgct
     4501 gcgacgagga tccggcgctc gtcgaggagc tgatcaagaa gcagattccg ctgacggtct
     4561 gcccactctc gaacttgaag ctgtgcgtcg tgaaggacct gcgggagcac aacttcgcga
     4621 agctcctgcg caagggcgtc gccgtgacga tcaacagcga cgacccggcg tacttcggcg
     4681 gctacatcgg cgagaactat cgcgcgacgg ccgatgcgct cgctctctcc gagcaagaga
     4741 tcatcacatg cgccgagaat gcggtgcatg cgtcgtttct gccgaacgcg cagaagctgt
     4801 cattgacgca agcgatccag ctagaggccc gtcgaggatg acactcgacg cggacgccgc
     4861 gaagattcca tcgctgatcg agcatgtcgc tgcgccgaaa gtcgcctcgc gctgggaaga
     4921 gctcgcgcag ctgggtgacc tcgcgtgcgg cggcgaccac gagccgtaca tcttcacggg
     4981 ctacagcggc ggcggcgaat cacacacagc cgttgctgcg ggccacgcga cttatttgaa
     5041 ggtcctggct ggcaaggatg cgaaggcgcg cgcggcagcc gcattcgtgc tcgcttggtt
     5101 gccggagcac gccaagaagt ccctcgcggc gctgcacaag gtcatcaaga aagagaagga
     5161 cgcgaccgcg cgcgcgagca tgatcctcgc ccttgccgtg ctcgcaccgg gcgccgacct
     5221 gaagctcgac accgaagcgc tcggctccaa agaagcaccg gtctcgggcg cgggcctcgt
     5281 cgctgctgcg atcgcgggcc agctgcctgc gaaggcaacg ttcgtcgcgc cgacgctcgc
     5341 tcgcgccgac acgatcgggg cgctgtgctg gcaacacggc gatctcggta cgctcgtcat
     5401 gcgcgcggtg ctgcacgcgt ccagtcgcga caacgacgcc accgtgctga cgaacatgat
     5461 cttcgccgag cgcggctatc tactgctgcc cgacgtgatc aaactgctgt ttccgtcgag
     5521 gatcgaggca gcgaagctga cgaagccgca gcgcgagctg ctcgacaagc tgacgctcga
     5581 tccgactcgc tacggctatg cgcagctacc caagctctgg gaggaacgcg gactcccgac
     5641 cgacgcggaa gccgcacgcg cgcaactggg cctcaccgag gtcgaagagc cactcgaaga
     5701 aatcatcgcc gtcggacgcg gcaagaagac gtttgccgag cacctcgctg aagcggtcgc
     5761 ggatcccgag cgtcgcaaac cactcgccgt cgcactcgcg aaggagaaca gcgcgagaga
     5821 agtcgtcgag ctgtgcgtcg aagggtgcac gcaggaagaa ggccctgatt ttcaggacac
     5881 cgtggtcgag ctgttcgaag cgtgcggccc cgatctcgtg cctgcgctcc gcgagctcga
     5941 catctacaaa gaggtccagc tcgacgacgg acgcgcgatt caaccgacgc aactcgccgc
     6001 gccggcgttg ctcctcctcg cgcgccgcga tcccgcgctt cccgacgaca agctcgatca
     6061 gctctgggcc gccgcatcgg cgcggcattt gatcgaaccg atcctcgcgg cgctaccgcc
     6121 cgatcgtcgc gcacgcaagc tggccgacgc gtatcgcaat gtcccgtcgt gggagatcgg
     6181 cgagaagacg cgcctcgaga agctcatgct cgcggcccct gcgcccgagc tgacggcgga
     6241 gctcctcgct catgtgaagg agctcaaaga cgcctatgcc aatggccgca agatggaggg
     6301 cacgctgttc tccgagggct actcgcccgc gaagctcaag aagctcaagc cgcgcgcaca
     6361 tccgctgacg ccgaagctga tcgagaacat cgaggcgtac gcccacgcca gcgacgacaa
     6421 gcaactcggc aaagcgatcg cccaattcct cgacatgtag cggtaagatc atcggcggtg
     6481 cgaaaccggc tgttcgtcct cgcgatcgtg ctttgcgcgg acgtcgcgca tgcggacgag
     6541 gtcgacgatc tcgtcgcgaa gggccaggag ctcgcgaagc agagcgagtt cacgcaggcg
     6601 atcgcgacgt tcaagcaagc ggatgcgaag cggccgcgcg ccgcgcacgc atgcatgatc
     6661 ggtctcgcgt acatgcgccg cgaagcatgg ccgcaggccg agctgtttct cgcgctgtgc
     6721 gagaagcgtg cagcgcccgg cgatcagccg cccgagtgga tcgacgaggc gaagaagcag
     6781 ctcgcgcaga agctgacggc tgcgcagatt cccgccgtca cgatcgacgt cacaccgccg
     6841 aacatcggcg cgcacctaac cgtctcgagc ttcgcgcccg acgaagtgtt cgacccgcag
     6901 acgattcacc tcgcgccggg tacacacgtg atcgaggtaa ccgcgccggg atatcgaagc
     6961 gagcgcaagg atgtcgtcgt cgagcccgcg aagccgcagc acgtgacctt cgagatgcag
     7021 cgagcaggcg acgcagtggc tcctgtctcg ccgcgacaac cggagcgtgg atcgcacagc
     7081 aaagtcccgt ggcttgtcgg tggcgcgggc gttgcgatcg gcgttgccgg catcgtctat
     7141 cacgcgacgt cggtgagcgc cgcgtacgac cgcgcagatg gcgccgagac catggccgcg
     7201 tacaacgtgc tcgtcgacga cttcaacagc aagcgcaacg tcgccgtcgg gctctatgca
     7261 gcgagctcgc tcaccatcgg tgttgccgtc gtgcttcgct ttacggtcta caaagacgcc
     7321 agtgagccac cgctcgtcac cgcacagctc ggtgaccacg gcgccggcat cttgatcggc
     7381 tggcagcgat gaagcagctt gcgctcctcg cgctgctcgg agggtgtctc acaccgccgg
     7441 ctgctcccgg aggtccggag agagcctgga cgcgcgtcgc cgacggcgct ctaccgggcc
     7501 gcatgcacgg cgcgaggctg acctgggatc cacagcgcga tgcgatcgtg atgtacacgg
     7561 gctacaacgc gacgcagaac ctcgcccccg gctacgtcta caagtggcaa gcgaacgagt
     7621 ggatgcgcat ctgcgaggag ggagtcggac cgcctccgct gtacttgccc ggcttcacgt
     7681 gggagccgag cgggcagctc gtcctcgtcg gcggcgctca gatcgttccc ggcgcgggtc
     7741 tgttcgataa cgtctccaaa gacatctaca cgtgcgacac gaccggcgtt tggacgaagc
     7801 agacaaacac gctgcagcga gagcgcgcgg gcgcaagcct gatctacgac ccgcagcggg
     7861 acaggctcat gctcgtcggc ggccgcgatc gcaacggtcc gatcaagaac gcggaaatgt
     7921 cagcgcccaa tgcgcaagac ttcaccatcc atccggccca gatgccgttc gcgagcgccg
     7981 gtgcggggca gaccgcgatg tacgacgccg actccaagat gatcttcgcg ctcgagggcg
     8041 agacgaccgg cgatgacgac acgccgttgc acgacgcgat ttggacgtac gacggcgccg
     8101 cgtggacgcg gttctgcaac gactgcagcg gtgcaccccg cgcggacgcg tcgatcgttc
     8161 acatccacgg gacgaacgag acgtacgtca tcgcaggatt tgccggaaat agccgcgacc
     8221 acgccgggac atgggtcatc gatcagaaca agctactgcg cgtgttcgac gagccagacg
     8281 cgcgtgcctc cgtcggagcg gcctacagcg acacgaccga ttcgatcgtc atgttcggcg
     8341 gcaccagcgt caactgcgaa gaatatgagt gcgacgcaac gttcgagctg agcatccaac
     8401 cgcaatgacg ccgcgcaccc tcgcccaact cgaagccgcg accgctcacg tgcgagcggc
     8461 gccgcgcgaa gagggcatcg tcacgttcat ggtccggcgt ccggacaaag gtgcacgcga
     8521 gatcctcgac gaggcgcagc tcgatacgcg cgccggcatg atcggcgacc gctgggtcaa
     8581 cagcacgagc aagcgcacgc ccgatggcca gccccatcca gagcagatgc tcacgctcat
     8641 gaacgtgcgc gcgatcgacg cgattgccga gcgcagctac tggccgcttg ccggtgacca
     8701 gttttacgtc gacttcgatc tctcgaaggc gagcttgccg cccggcacca cgctcgcgat
     8761 tggcaacgcg ctcgtcgtca tcacgccgat cccgcacacc ggctgcgcga agttcaccga
     8821 gcgattcggc agcgacgcga caaagtgggt gaacagcgac gagggtctcg agcttcgcct
     8881 gcgcggcgtg aatgcgcgag tgatgcgtgg cggcgtggtg cgcgtcggcg attcgctgcg
     8941 ccgcacgtag ctacgacgcc gcgagcacgc cgtcggcgat cgcgatcgcg cggactgcat
     9001 cgatcgtgtc ggcctgcacg gcggtcttgt catcgcgata gcgcacgacg cgcgcgagcc
     9061 gcagcgcgag gcccgctgga tagcgcggcg atcgctggac gtcgttgaac gcgatctcgg
     9121 caacgacctc gggcttcacg tagacgatgt ggccatcgcg atcgacctcg agtgagagga
     9181 acttctcggt ctgccacttg agcatctcgt cggtcatgcc cttgaacgtc ttgccgagca
     9241 tcacgtagtc gtcgccgctg cgcgcaccga ggtggatgtt cgagagccag cccttgcggc
     9301 ggccactgcc ccactccgcc gcgagcacga cgagatcgag gcgatggacc ttcttgatct
     9361 tcagccacga cgcgccgcga ttaccggcgt cgtacggagc gtcgagcgcc ttggccatca
     9421 cgccctcgtg gccgctagcg attgcctgcg cgtagaacgc ggacgcctcc tgcggatccg
     9481 acgtgatcaa tcgcttcacc gaatgcgcag gcgcgagcgc atcgagctcg tcgagacgac
     9541 gcttcgcggg ctcggtcatg agcgtttggc cgtcgatgag cagcgtgtcg aacagcgcga
     9601 gcgagagcgg catcttgtct gcgagcagcg ggccgttctt gccgaagcgc ttcatcgtgt
     9661 cctgaaatgg caacggccgg ccgttcgcgc cgaacacgat cgcttcgccg tcgacgatca
     9721 gcttttgtgc gggcagcttt gcaatcgccg caatcgcctc gggcacgtag tcggtgacct
     9781 cgttgagcgc gcgcgaatac gccttcacga tctcgccgtc cttgtgaatc tggacgcgaa
     9841 agccgtcgag cttgtactcg agcgacgcgg ggccgccaaa cgtcgtcagc gcatcggctg
     9901 cgtcgtcagc ggtctgcgcg agcatcggca gcacggggcg aaacagcgtg aggccgaagc
     9961 ggccgatctc ggcggcaccg ccttcgagcg cggcggctgc gacctctccg agatcgcccg
    10021 cgagcatgta cgcgcgacgc accgaatcgg cgggcagctc ggccgccttc gcgatcgcct
    10081 cgacgacgag accatcgagg gctccttgtc gcacttcacc gacggcaagc ccgccgagaa
    10141 acttctgctc ggcggccgtc gcgagcgaca gcagctggcc gaaatgatcg cgtcgcgcgt
    10201 tcgccgaacc ggtgccgcga agctcggcga tcgcggtgaa gcgccggtcg acatcgccga
    10261 gcgccagata tgcagcgacc gcgggcatcg cttgccgcat cacctctgcg accgtcgcgt
    10321 atccgatgtt cgtcttctgc ggcagctcac cggcgaggta gcgcgcgccg atcgcacgct
    10381 cttctgcttc catctgccgc aagcacttcg cgagcagctc gatcttcttc agccggccgc
    10441 tggtctcggt gacctgcgcg ctggtagcga cgagctcggc gagcagcacg ccgacacctt
    10501 agctcagttc gtcgcgactt cgagcttcca gctgttgagc gtgccgacgt cctgcgatgc
    10561 agtgtcgacg accttcagcg tccacgtgcc tttgagctct ccggagaagc cgctcacggt
    10621 gaacgtctgc ttgagatcgt cggcggaacc accttcgccg ttgaagacgg tgaccgtcgt
    10681 cgcgccgtgc gtcagcgtaa ctttgagatc accgcggtac gtgtgcgtga cgtcggtcgt
    10741 gagcttgacc gatgcgagcg tgcccgcgtc ggccacttcg atcgtgctcg acacgccggc
    10801 aggcgcgttg tcgggaatcg cggacgccgg catcgcggag tagctgtgcg tctgaccacc
    10861 cgacggcggc ggaggcgtcg gaccgttcag caccggcact tcgccgacga ccgccgagcc
    10921 gtactccttc acgtagcgca tcgacagcca gccgtagccc gcgccgtatg gatgatcgat
    10981 accgaagccg gccgtgcccc acgagttctt gaacaggaag aagcccttct ctttcttggg
    11041 ctggccgttc gcgtcgagga tcggcttgcc ttcgccatcg cgcatcggga tctcgaggtc
    11101 gtcatcccaa ccgatgatct cgatcgcgtg acccgcgcgg tgcgttgccg actcggtctt
    11161 gtccttgtcg tttggatacg tgacgaagcc ctggcgccac agctcgctgt cgaccggaat
    11221 cgtcgagcgg cggtgattcc acgattgata gaagaagttc aggccgacgc cgacgccggt
    11281 cttcttcgtc gtcaggtgtg ccttgatcga gttcgtgttg atccagcgcg acgacggcag
    11341 cttgaacttc ggcgcatcga gcgcggtctg cggcggctcg ccgttcgtgt agcacttcgt
    11401 cggtaggctc tcgccgcccg tgcactcggg atcatttgct gcggtccacg gcgccgattc
    11461 gtacgcccag tctgattcct tgatgtcgcc gaactcgacc accgcttgga ggttgtcgtc
    11521 gacgttgctg ccctcggtgt gctggaacgc accgacttgg ttcttcaccg accactgcat
    11581 gtactgctcg gagaagtcga cctcggccgc gggcatgccg gccttcaggt agaggttctc
    11641 gacgatcgca gtcgccgcaa agatcgagca cacgccgcgg ctgccctggc tcttcaccgc
    11701 ggactgctcg ttcttcggca gctcgaactt cgcgggataa acggcgtcag ccttgttgtc
    11761 gtcggggagt gagtcgttgt tcggcgcgcc ctcgttgatg ttgccgggcg tcacgccgta
    11821 atcgtcgtcg tacggggtcg gaccatcgtc ctgcatgtcg caggcaccga tgagaagagg
    11881 aaggctgaac agcaggacct tacgcatgcc ggctggccac tgcggaagtc atgccgaaag
    11941 accccagctg caaccccgca ttttgcggga cagcctgggg tctgtggcca gccgactagc
    12001 cggctaccgg acgagtgaat gtggttagaa cacgaacggg tagctgaacg agccaccgtt
    12061 ctgcgtcttg ccgaacgagg ccttctggac ggccgccgcg acgcacgcgc cgagcgccga
    12121 atccggcgtc gtctcgaccg caacgttgct cacgcgacca tcgccaccga ccgacacctt
    12181 caccttcact ttgcccttcg ccggcgactt gtcaccgcac gcttggacac gcgccttcac
    12241 gttcgcgatg ccagacgaga tcatcgagcg gtcgaggccc tcgggcagat cgctcttgcc
    12301 accaccaccg ccgccaccac cgccaccacc ggtcttcggc gcgccgccct tcttgaactt
    12361 cgcgcagcat gcaccttcgt agttgttgag cacgcacgag acttcgtcac agccaccacc
    12421 gccgccggtc tccgtcttcg tcgtggtcgc tgccgtcgtc gtggtgctgc cgccgccacc
    12481 ggtcttcggc gtcgtcgtgc cgccaccacc ggtcttcggc gtcgtcgtcg agccaccacc
    12541 gcccgtcttc ggcgtcgtgt gcttcgtctc ggtcgtcttc gtctcggtcg tcttggtctc
    12601 gggcgtcttc gtctcggtcg tcttcgtctc ggtcgcgctg cccgccgtcg agcccgtgtc
    12661 ggtcttggtc tcggtcgcgc tgcccgcttc gggcgtcttg acctcgtcct tcttgtcgtc
    12721 cttcgtctcg tccttcttct cttcggtatc ggtcttcggc gtggccgacg cgacctgcgg
    12781 gttctgtttt ccgcccttga ggaggaggac gaccataatg atcgcgacga cggccagcag
    12841 gccgaccgca ccgatcatga gcatgagcat cttgttgttc gccgcgggac gcgcggtcgg
    12901 aacgatgacg gcgggctcgc tgaagccacc accaccgaac accggcagat catcgatcga
    12961 gccgatgccc gacgacgccg atgccggctt gccgacggct gcaccgccgc ccgatccacc
    13021 gaggtacgcg ctcgccatcg agcgaatgtc gatgaggccg gagccttcgc cacccgctgc
    13081 gcccgttgcg tgacccgacg acgacgagcc cgaggagccc gacgaggacg acggagccgc
    13141 tgcgcgcggc gtgtcactcg cgagctgcgc gaggttgttc agcgagaaca gcacggagtt
    13201 ctcgttgcgc tcgccgcgaa gcttctgcga cgaggccgac gctgctgccg acgaggagga
    13261 cgatgccgac ggcttgctgc cgaacagatc gccgccgtcg tcttcatcgc gcgagctgcg
    13321 cgacgagccc gagctcgagc ttgcggctgc gaacagatcg cctggatcgc tgcggctcgt
    13381 accgctgtcg tccgtgccga tgccgccgct gccgaacatg ccggcgaccg ctgctgcgcc
    13441 accgccgctg ccggccgtcg tcgtcgatcc cgatgcgacc atcgcggaga actgatcgac
    13501 ctgcgcgagc ggtagccagt cggcaaagcc ttcacgccag gtgaacgtct cgccgtcgac
    13561 ttcgccggac gcgaacttcg attggacctc ggcgacgctc agcgggccga cttgatcttg
    13621 gttgatgacg agatgccaga ctgcatcgtc gccaccgcct ccaccaccag cgccaccttc
    13681 gtagccgtag tcgtaaacgc gggtgtcctt ctcggctgga gcgggcgcag cttcagccgc
    13741 acctgcaccg ccgcgaacga cgatgatgtt gctgcacttc ttgcagcgaa ttttgaagac
    13801 cttgccttga accttgtcgt cgctaatcga gtacttggct tggcaggcgt cacagacgat
    13861 cttcatacgc tttccctgtt tcgcggttca caccctacga cccccaagtg aaccgacggc
    13921 ttggagaaaa gatcgtatca caaatcgccg ggttgcctca gtttgcgaac ggatccatga
    13981 ggcggatcgt ttcggcgcga tcggggccga ccgagaccag gcagaacggc acgcctgcca
    14041 tctgcgagat ggtctgaacg tagctgcgcg ccgacgctgg taggtcttcg atcgagtggg
    14101 cgtcggcggg gagcttgtcc cagccgggga accgctggaa caccgcttcg gcgcgcgcca
    14161 aatcctctgg atccgtcggc ggctcgtcga gctccttgcc gtcgagcttg taggcgacgc
    14221 acacggagac ctcgccgaga cccgccagga catcgagctt cgtgagcgcg agcgactgca
    14281 tgcccgagag ccgaacgccc atgcgcagcg cgggtagatc gagccaacca cagcggcggg
    14341 ggcggcccgt cgtcgcgccg tattcgccgc ctgctttgcg cagccgctcg cccatctcgc
    14401 cgtgcagctc ggtcgggaat ggcccctcgc cgacgcgcgt ggcgtacgcc ttcgtgatgc
    14461 cgacgacgtg atcgatctgc gtgggaccga tgcccgcgct ctgacacgcg cctgccgcga
    14521 tcgtcgagct gctcgtcacg tacggatacg ttccatgatc gagatcgagc aggcagcctt
    14581 gcgcgccttc gaacagcacg tgcttgtggt cggcgatcac gttcgccacg aaccggccgg
    14641 catcgcccgt gtacttgcgc agcttctcgc ccgcggcgag cgcgtcgttg atccattgat
    14701 cgatctcggt cttcggcggc acggcgccac cgaggtgctg gatcagcggc gcgagatcgt
    14761 cgaggttcgc gctgacgagg ttgtgcaggc gctcgggctt cagcaggtcg cgcatgcgaa
    14821 tgccgcggcg cgcagctttc atctcgtacg ccggaccgat gcctcgacgc gtggtgccga
    14881 tcgctttgcc gctcttcgcg ccgtgatctt cgcgcaggcc gtcgatgagc ttgtggtacg
    14941 gcaggatcac gtgcgcgccg agaccgatga ggagctcgtt gcgcatgagc aggccgcgct
    15001 cctgacactc cgcgatctct tcgagcagcg tgtgcggatc gatgaccatg ccatcgccga
    15061 gcacgcac
//
LOCUS       DATLBZ010000208        12485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5422793, whole genome shotgun sequence.
ACCESSION   DATLBZ010000208 DATLBZ010000000
VERSION     DATLBZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12485
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5422793"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..2191
                     /locus_tag="VMZ53_10970"
     CDS             134..2191
                     /locus_tag="VMZ53_10970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HUS29028.1"
                     /translation="MTKLSLIAVLVLSAVTSCKRPGKGSYFATVGPTGPTHPVPKPAV
                     VETPSDVTWLEQCRGPIDTAKTQIAQILAVQGVRNATNTLDVYNEASRQLNNAGEWAH
                     LHAAVNPDAKIRDAGRVCEQDVQKFVSNLMLDNRVYTAIKSVDVSLADANTKRFVTVT
                     LRDFKRAGVELDDTGRTRIKQIDEEETRLGQQFQKNVDEDVRKVEIKDASQLAGLPPD
                     WVAAHKPDASGVIRVSTDYPDYIPFMTYAESDELRKQLYIAFRSRGDAKNEELMQKIL
                     TLRAEKAMLLGFKDWSDYQSDDKMLKGGKAAGEFLDRVNTVALRRVKKDYAELLAQLK
                     KKVPAATQVDDWQRMYLENQLRKDKYAVDSTEIRKYFPYEQTLKGLLDITSALYDITY
                     VPVEKDPRVWAPDVAVYDVMQKGQKLGRIFLDMHPRTGKYKHAAQYPIVDGVKGVQLP
                     EGALICNFADPATTKPALMEHADVVTMFHEFGHLMHHILGGQQHWIRQSGVATEWDFV
                     EAPSQMFEEWAWNYDVLSRFAKHAETGAVIPKELVEKMRKADRFGKGLWATQQLFYAA
                     LSLKFHQETPAKLNQLELLKTLQKKYTPFAYVEGTKFHTSFAHLVGYSSMYYTYLWSL
                     VIAKDLQSAFEKKGLLSTDVSYAYRDKILAAGGTKDAADLVKDFLGRPYNFKAFEKYL
                     SEQ"
     gene            complement(2192..2692)
                     /locus_tag="VMZ53_10975"
     CDS             complement(2192..2692)
                     /locus_tag="VMZ53_10975"
                     /inference="COORDINATES: protein motif:HMM:NF025918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HUS29029.1"
                     /translation="MTSQTIQLVERVRQRLDHQDNPVRIKGPVSEAAITQAEEQLDCT
                     FPPSYRAFLRTWGGIVLPTHLGIVHDFIGVAPNDSDGDVVARTLRAREERKLPEHLVV
                     VGMGAQHQEWFCLDLSKSLPNGEYPVMMYDARDNALDQQFYDDFGQMLEEVMGFVADS
                     LEQPLD"
     gene            complement(2758..3648)
                     /locus_tag="VMZ53_10980"
     CDS             complement(2758..3648)
                     /locus_tag="VMZ53_10980"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUS29030.1"
                     /translation="MQRSVKDLLGGGLKPNDPRRFLIEAMVAAMSADGHIDARESAMM
                     EQQIANHAFFAGLGAKSAKTLVELSTDAIRFAGGAIPRTPAIAKGLPARIHRLAAFGM
                     AAEVVVADQRLESGELSFLEALRIALRIAPNEAEDIINAARVGQLPPYLEDRYLRVKS
                     MVSVAAEVFALRALARAAANDEQRFKVRDFFLAIPDLALSTDELDAELYRAFRRPRAP
                     DAQVFGELSRVASTLPGPVDRYWMVVYTLVAESPASVPSWRVIPFMGVMQAAFQIVDT
                     DMELAVIDALTFPANLPRPA"
     gene            complement(3658..4521)
                     /locus_tag="VMZ53_10985"
     CDS             complement(3658..4521)
                     /locus_tag="VMZ53_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29031.1"
                     /translation="MGLIGHELVGHGGMALACGGTIDAVQLFWFAGGWIRYHLSEPSQ
                     AATLAIAMAGIALELVVGIVLWFAVRGDTLGKRITRGVGAALVLHAGWYLATGAFHGF
                     GDGLVLYRMLGSWRTPVALGAGMLTCIAGFAGAREVLGAIAGTLPARGRIAGTIVALL
                     LAAGVHGALTAGELHLRRDPTYANVMRPERDRVIAKELAQWQTEQQQRGTLVTEEQKR
                     IEQRRLAKVHRSFPFVWLLGAATGIAILLGVRRAKPATDSRITNRLLFITTTIAVASV
                     LAVIVLDALVV"
     gene            4613..5983
                     /locus_tag="VMZ53_10990"
     CDS             4613..5983
                     /locus_tag="VMZ53_10990"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS29032.1"
                     /translation="MRRLVVAGLLCVSALGGTAAAEQTRKVHVTTEPPNATVYLNDTD
                     SGPKCDQTPCDIDAPIGSPTIIIRLDKYEPVVEPLDVAKGKKPMNAHFKLKSAIGTIV
                     ISQPKGATVQIDGEDQGTIGSSPLRADVSAESHIVVITLKGKTFFDDAVDIQTGDELD
                     LGPDTPGWKSSGVVASAGGGGGDGDGDGDTGGGGVGDGDGGGSGDGGDTGVIGSTDVV
                     AHDHILSVGLAFDVGFRRFSYDMPQGMNLKPESEDGQVIIGPAVEVFPMRALKINALQ
                     GLSLFARAQFGVHGQQLTGGMIKGTVTTFWASYEGSLRYRRRITGGFHIEGSGGFVRD
                     QMQFNATDGDDIKLVPDATYDSLRLGVKLMIVSGKLEAYLGAENRIVLGGGTLATRFD
                     SAHATGLRGVVGATYTMGKLFIRGEGALMHYSWTFASTGGSGTEMADGATDSVRLISF
                     LAGYRY"
     gene            complement(5984..7414)
                     /locus_tag="VMZ53_10995"
     CDS             complement(5984..7414)
                     /locus_tag="VMZ53_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS29033.1"
                     /translation="MGTKQGKILVVDDDQDTAELLRDALAKRGWDVEAARSGMEALAR
                     FTRQPADVVVTDVQMPGMSGIELCQEIRATGMETLPIVITGVGNLDVAIGAMRAGAFD
                     FIQKPVKVDVLAIAIARALDHLALRRELKKLHSQALDAFMTDGIVGNSPAIRTTIEMV
                     HRVANSDATVLITGDSGTGKELVARAVHNLSPHRDKPFIAVNCAAMPAPLLESELFGH
                     VRGAFTDAKTSRAGLFVQAGSGTIFLDEIGEMPLEMQVKLLRVLQERKVRPVGSDEEI
                     PIHARVVTATNRDLEQEVEERRFREDLFYRINVVAIPVPPLRERPSDILVLAQHFIRR
                     AALRSKKDVRGIGEHAARYLLGYDWPGNVRELENSMERAVALCKLDQITIDDLPDKLL
                     QDHKSKMVISAEPSELISLAEMERRYVRQVLNAVDGNKTRAAQILGIDRRSVYRRLEG
                     LNQQQAAAAPPPPTGGDGAPPAPAAS"
     gene            complement(7684..9270)
                     /gene="pckA"
                     /locus_tag="VMZ53_11000"
     CDS             complement(7684..9270)
                     /gene="pckA"
                     /locus_tag="VMZ53_11000"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HUS29034.1"
                     /translation="MAAFSLEKQGISVAEIHRNATPAFLYEAALKFEKGTAISSTGAM
                     VAYSGKKTGRSPTDKRVVDEPEVRDEVWWGNVNVKLEPQSFVINRERAIDYLNTRDRL
                     YVVDGYAGWDEKYQLKIRVICARAYHALFMHNMLIRPTDKQLESFGEPDYVIYNAGGF
                     PANRHTQGMTSSTSIDLSFGRREFVILGSEYAGEMKKGVFTIMNYLMPKQGVLSMHCS
                     ATQARDGSDTSILFGLSGTGKTTLSADPKRLLIGDDEHCWTDDGVFNIEGGCYAKAIK
                     LSKESEPDIWNALQFGAVLENVVLDPYTRVVNFDDTSITENTRGSYILDTIPNAKLPA
                     VGGHPKNVIFLTADAFGVLPPVSKLTPAQAMYHYISGYTAKVAGTEVGVKDPEPTFSP
                     CFGGPFLVWHPTKYAEMLAEKLRKHGAQTWLVNTGWSGGAYGVGSRIKLKYTRAIIDA
                     IHSGALSNVPTVEDPIFGFAVPQSCPECPSEMLQPRNTWADKAAYDEKAKKVASLFKE
                     NFKKYEAQASTEVRGAGPKV"
     gene            complement(9291..12341)
                     /locus_tag="VMZ53_11005"
     CDS             complement(9291..12341)
                     /locus_tag="VMZ53_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HUS29035.1"
                     /translation="MNQKAVEFETFPLGDTSGTRRTMDCDYGTAVTAASIANVDAFEP
                     HTAEGIDLYAQNEFRCESSTTYDVTGTKISNDLLLPKTAMIHAIGLTADNYGAMALKN
                     TALIWSPRSNITLYGDTARVTTAHRFGVEIALGTDWTPTGSMNMLRELRCADSLNKTY
                     YDNYFTDKQLWEMTTLNAAAVTATDDVIGLLAPNHVADISIFKGKAGGPFRSIIDADP
                     QDVVLVMRGGKALFGDDASIAALSSDCDTIDVCGTGKRVCLKSEIGKTLAEVQAAVGT
                     IYPAFFCAVPDKEPSCTPKRPAAVSGSTIYTGAISADDSDGDGLPDTADNCPKVFNPI
                     RPMDGGKQPDTDNDMAGDACDPCPTDANTTACTPVNPDDRDHDGFINSADNCPDTANP
                     GQSDGDMDGHGDACDACPSNANPGTAGCPATIYAIKKGEVSTGTTVRIENALVTGKGT
                     NGFFMQTKAGDAGYMGADFSGIFVYTGAMAPTLAKATVGLRVTVDGKVTNFQNQLELD
                     TVASVTPTTMVGEAAPTPVATTYADVKTGGPKAAAYEAVLISVGAATVSALNATFGEY
                     TLTAGSDTLIVDDFLYVTPANGSAVGTSYSTVIGILALRQQASKLEPRSAADLVQGTP
                     TLAAFGPALGFVRAGQSGVNSIPAGMELTVTLSGPAQGNTDVTVSSPSGDVVVVGGKV
                     TVLNGETTAKVVLDGVNQNTTPITLTAQLGGGAAKTAQVRVLGANEAPTSVSIAPTST
                     AVAPLGSATFTATLDIPAPPGGTSLTLGVSPGTSGSTNPATTLVIAADTLSNTFVYGD
                     ATGANATVSAALSGGNTANATVTVNAGASHLVINEVDYDNTGAVPPNDNAEYIEIYNP
                     TSSAVSLANVALVLINGSTGDAYPNADSIIDLSSAGSIPAGGYLVVAGQALAVAPPAL
                     KFEPGWTTDGIQNGNPDGVALVDTSTNTLLDALSYEGSVTAANVPGIANPVSLVEGTA
                     LSTSIADSNTVLGSLCRSPNGRDTDSASADWKFCTSLTPGTANP"
BASE COUNT         2184 a         3968 c         4127 g         2206 t
ORIGIN      
        1 ccgtcgatcc acactcgccg ccaaagtttc gcgtctacgg cgcgctccgc aacctgccgg
       61 agttcgcgca ggccttccag tgtgcagcag gaacgccgat gcgtccgtcg gagacctgct
      121 cggtatggtg aacatgacaa agctctcgct gatcgctgtc ctcgtgctct cggctgtcac
      181 gtcctgcaag cgtcctggca aggggagcta cttcgcaacg gtcgggccga ccgggccaac
      241 gcatcccgtt ccgaagccgg ccgtcgtcga gacgccctcc gacgtgacgt ggctcgagca
      301 gtgccgcggc cccatcgaca cggcgaagac gcagatcgcg cagatcctcg ccgtgcaggg
      361 cgtgcgcaac gcgacgaaca cgctcgacgt gtacaacgag gcgtcgcgcc agctgaacaa
      421 cgccggcgag tgggcgcacc tgcacgccgc ggtaaatcca gacgcgaaga tccgcgacgc
      481 ggggcgcgtg tgcgagcaag acgtccagaa gttcgtctcg aacctcatgc tcgacaatcg
      541 cgtctacacc gcgatcaagt ccgtcgacgt ctcgctcgcg gacgcaaaca cgaagcggtt
      601 cgtgaccgtc acgctgcgcg acttcaagcg cgccggtgtc gagctcgacg atacgggccg
      661 cacgcgcatc aagcagatcg acgaagagga gacgcgcctc ggccagcagt tccagaagaa
      721 cgtcgacgag gatgtccgca aggtcgaaat caaagacgcg tcgcagctcg ccgggctgcc
      781 gccggattgg gtcgcggctc acaagcccga cgcaagcggc gtgattcgcg tgtcgacgga
      841 ctatcccgac tacatcccat tcatgacgta cgcggagagc gatgagctcc gcaagcagct
      901 ctacatcgcg ttccgcagcc gcggcgatgc gaagaacgaa gagctgatgc agaagatcct
      961 gacgctgcgc gctgagaagg cgatgctgct cggattcaaa gactggtcgg actaccagtc
     1021 ggacgacaag atgctcaaag gcggcaaggc cgctggcgag ttcctcgatc gcgtgaacac
     1081 cgtcgcgctg cgccgcgtga agaaggacta cgccgagctg ctcgcgcagc tgaagaagaa
     1141 ggtgccggcc gcgacgcagg tcgacgactg gcagcgcatg tacctcgaga accagctgcg
     1201 caaggacaag tacgcggtcg actcgacgga gatccgcaaa tacttcccct acgagcagac
     1261 gctgaagggc ctgctcgata tcacgtcggc tctctacgac atcacgtacg tgcccgtcga
     1321 aaaggatcca cgcgtgtggg cgcccgatgt cgctgtctac gacgtgatgc agaagggcca
     1381 aaagctcggc cgcatcttcc tcgacatgca tccgcgcacc ggcaagtaca agcacgctgc
     1441 tcagtacccg atcgtcgacg gcgtcaaagg cgtgcagctg cccgagggcg cgctcatctg
     1501 caacttcgcc gatccggcga cgaccaagcc cgcgctcatg gagcacgccg acgtcgtcac
     1561 gatgttccac gagttcggcc acctcatgca ccacatcctc ggtggccagc agcactggat
     1621 ccgccagagc ggtgtcgcca cggaatggga cttcgtcgag gcgccgtcgc agatgttcga
     1681 ggagtgggcg tggaactacg acgtcctctc gcgcttcgcg aagcacgccg agaccggcgc
     1741 ggtgatcccg aaagagctcg tcgagaagat gcgcaaagcc gaccggttcg gaaagggcct
     1801 gtgggcgacg cagcagctgt tctacgcggc gctgtcgctg aagttccacc aggagacgcc
     1861 cgcaaagctg aaccagctcg agctgctcaa gacactccag aagaagtaca cgccgttcgc
     1921 gtacgtcgaa ggcacgaagt tccatacgag cttcgcgcac ctcgttggct actcgtcgat
     1981 gtactacacg tacctgtggt cgctcgtgat cgcgaaggac ctccagtcgg cgttcgagaa
     2041 gaagggcctg ctctcgaccg acgtgagcta cgcgtatcgc gacaagatcc tcgcggctgg
     2101 cggcacgaag gacgccgcgg atctcgtgaa ggacttcctc ggccggcctt acaacttcaa
     2161 ggcgttcgag aaatacctct cagagcagta gtcaatctag cggttgttcg aggctatcgg
     2221 cgacgaagcc catgacctct tcgagcatct gcccgaagtc gtcgtagaac tgctggtcga
     2281 gcgcgttgtc gcgcgcgtcg tacatcatca cggggtactc gccgttcggt agggacttgg
     2341 acaggtcgag gcagaaccac tcctggtgct gcgcccccat cccgacgacg accaggtgct
     2401 cgggcagctt gcgctcctcg cgggcacgca gcgtgcgggc aacgacgtca ccgtcgctgt
     2461 cattcggcgc gacgccgatg aagtcgtgga cgatacccag atgcgtcggc agcacgatgc
     2521 cgccccatgt gcgaaggaac gcgcgatacg acggagggaa cgtgcaatcg agctgttctt
     2581 ccgcctgcgt gatcgcggct tcggatacgg gtcccttgat gcgaacggga ttgtcctgat
     2641 gatccaatcg ctggcgaacg cgttcgacga gctgaattgt ctgcgacgtc ataagcgggt
     2701 cgttcgatta tgcacgctcg ctccgaaaaa ccagtcgcag atttgcgcga tcttttttca
     2761 cgccggacga ggaagatttg cgggaaatgt caaggcgtcg atcaccgcga gctccatgtc
     2821 cgtgtcgacg atctgaaatg ccgcttgcat cacgcccatg aatggaatca cgcgccagct
     2881 cggaacggac gcgggagatt ctgcgacgag cgtgtagacg accatccaat agcgatcgac
     2941 gggaccgggc agcgtcgaag cgacgcgcga cagctctccg aaaacttgtg cgtccggtgc
     3001 gcgaggacga cgaaacgcgc gatagagctc cgcgtcgagc tcgtcggtag atagcgcgag
     3061 atcgggaatc gcgaggaaga aatcacgcac cttgaagcgt tgctcgtcgt tggcagcggc
     3121 tcgagcgagt gcgcgcaggg cgaacacctc ggccgcgacg gagaccatgc tcttgacgcg
     3181 caggtagcga tcctcgaggt acggcggaag ctggccgacg cgcgccgcgt tgatgatgtc
     3241 ttcggcctcg ttcggcgcga tccgcagcgc gatgcgcagc gcttcgagaa acgacagctc
     3301 gccggactcg aggcgctgat ccgcaaccac gacttccgcg gccatcccaa acgcagcgag
     3361 acgatggatg cgggcgggaa ggcccttcgc gattgcgggc gtgcgcggga tggcgccgcc
     3421 ggcgaagcga atcgcatcgg tcgagagctc gacgagcgtc ttcgcggatt tcgcgccgag
     3481 gcccgcaaag aacgcatgat tcgcgatctg ctgctccatc atcgcgctct cgcgcgcgtc
     3541 gatgtggcca tccgcggaca tcgccgcgac catcgcttcg atcaagaacc ggcgtgggtc
     3601 gttgggcttg agcccgccgc ccagcagatc cttgacgctt cgttgcatgg gccgagctta
     3661 cacgacgagc gcatcgagga cgatcaccgc caagacagac gcgaccgcga tcgtcgtcgt
     3721 gatgaacagc aagcggttcg tgatgcgcga atccgtcgca ggcttcgcgc gccgtacacc
     3781 gagcaggatt gcgatgcccg tcgccgcgcc gaggagccac acgaatggaa acgagcgatg
     3841 caccttcgcg aggcgccgtt gttcgatgcg tttctgctcc tcggtcacca acgtgccgcg
     3901 ctgctgttgc tccgtttgcc actgcgcgag ctccttggcg atcacgcgat cgcgctcggg
     3961 ccgcatgacg ttcgcatacg tcggatcgcg acgcaggtgc agctcgcccg cggtgagcgc
     4021 tccgtggacg ccagccgcga gcaagagcgc gacgatcgtt ccggcgatgc gtcctctggc
     4081 gggcagcgtt ccagcgatcg cgccgaggac ctcgcgcgca ccagcgaatc cggcgataca
     4141 cgtcagcatg cccgcgccga gcgcgaccgg cgtccgccaa ctgccgagca ttcgatagag
     4201 cacgaggcca tcgccgaacc cgtggaacgc acccgtcgcg agataccagc ctgcgtggag
     4261 cacgagcgcc gcgccgacgc ctcgcgtgat gcgcttgccg agcgtgtcgc cgcggaccgc
     4321 gaaccacagc acgatgccca cgacgagctc gagcgcgata cccgccatcg cgatcgcgag
     4381 cgtcgcggcc tggctaggct cgctcaggtg gtatcgaatc cagccgccgg caaaccagaa
     4441 cagctgaaca gcgtcgatgg tgcctccgca ggcgagtgcc attccgccat gcccgacaag
     4501 ctcgtgcccg atgaggccca tacggctcgt cgcaagtgct gcgcacgcaa gcaaaccggc
     4561 gaggcgtaag acttccttgt gcatcggggg aggcgggtta tcatcattat ctatgcgacg
     4621 gctggtggtc gccggcttgc tgtgcgtttc cgcgttgggc ggtaccgcgg cagccgagca
     4681 aacgcgcaag gtccacgtga cgaccgagcc gccgaacgcg accgtgtatc tcaacgacac
     4741 ggactcgggc ccaaagtgcg atcagacgcc gtgtgatatc gacgcgccga tcggcagccc
     4801 gacgatcatc attcgcctcg acaagtacga gcccgtcgtc gagccgctcg acgtcgcgaa
     4861 gggcaagaag ccgatgaacg cgcacttcaa gctgaagagc gcgatcggta cgatcgtcat
     4921 ctcgcagccc aagggcgcga ccgttcagat cgacggcgag gatcaaggca cgatcggctc
     4981 gtcgccgctt cgcgcggatg tctcggccga gagccacatc gtcgtcatca cgctgaaggg
     5041 caagacgttc ttcgacgatg ccgtggatat tcagaccggc gacgagctcg acctcgggcc
     5101 cgatacgccg gggtggaagt cgagcggcgt cgtggcgtcg gccggtggtg gtggcggcga
     5161 cggcgacgga gatggcgaca ccggcggcgg gggcgtggga gatggcgacg gcggtggaag
     5221 cggcgatggt ggcgacaccg gcgtgattgg ctcgacggac gttgtcgcgc acgaccacat
     5281 cctcagcgtc ggcctcgcgt tcgacgtcgg gtttcgccgc ttcagctacg acatgccgca
     5341 gggcatgaac ctaaagcccg agtccgaaga cggccaagtg atcatcggtc ccgccgtcga
     5401 ggtcttcccg atgcgcgcgc tgaagatcaa cgcgctgcag ggcctgtcgc tgttcgcacg
     5461 cgcgcagttc ggcgtgcacg gccagcagct caccggcggc atgatcaaag gcaccgtcac
     5521 taccttctgg gcgagctacg agggcagcct ccgctatcgc cgaaggatca cgggcggctt
     5581 tcacatcgaa ggcagtggcg ggttcgtgcg cgaccagatg cagttcaacg cgaccgacgg
     5641 tgacgacatc aagctcgtcc ccgatgcgac gtacgactcg ctgcgcctcg gcgtgaagct
     5701 catgatcgtg agcggcaagc tcgaggcgta cctcggcgcc gagaatcgca tcgtgctcgg
     5761 cggcggaacg ctcgcgacgc gcttcgattc agcgcatgcc accggcctgc ggggtgtcgt
     5821 cggcgcgacc tacacgatgg gcaagctctt catccgcggc gaaggcgcgc tgatgcacta
     5881 ctcgtggacg ttcgcgagca cgggcggctc gggcaccgaa atggccgacg gcgcgaccga
     5941 ctcggtgcgc ctgatctcgt tcctcgccgg ctatcgttac tagctagcta gcggcgggcg
     6001 cgggcggggc gccatcacca cccgttgggg gcggaggggc ggcagcggct tgctgctggt
     6061 tcaggccctc gaggcggcga tacaccgagc ggcgatcgat gccgaggatc tgcgcggcgc
     6121 gcgtcttgtt cccgtcgacg gcgttgagca cctgacggac gtagcgacgc tccatctcgg
     6181 ccagcgagat cagctcgctc ggctccgcgg agatgaccat cttgctcttg tgatcctgca
     6241 ggagcttgtc cggcaggtcg tcgatcgtga tttggtcgag cttgcacaga gcgacggcgc
     6301 gctccatcga gttctcgagc tcgcgaacgt tgccgggcca gtcgtagccg agcaggtagc
     6361 gcgctgcatg ctcgccgatg ccgcggacgt ccttcttgct gcgaagcgcc gcgcgacgga
     6421 tgaagtgctg cgcgaggaca aggatgtcgc tcggacgctc gcgaagcggg ggcaccggaa
     6481 tcgcgacgac gttgatgcga tagaacagat cttcgcggaa ccggcgctcc tcgacctctt
     6541 gttcgagatc gcggttcgtc gccgtgacga cgcgcgcgtg gatcggaatc tcctcgtcgc
     6601 tgccgaccgg ccgcaccttg cgctcttgca gaacgcggag cagcttgacc tgcatctcga
     6661 gtggcatctc gccgatctcg tcgaggaaga tcgtgccgct gcccgcctgg acaaacagac
     6721 cggcgcggct cgtcttggca tccgtaaacg cgccgcgaac atggccgaac agctcgctct
     6781 cgagcagcgg tgccggcatg gccgcgcagt tcaccgcgat gaacggcttg tcgcggtgcg
     6841 gcgagaggtt gtgcacggcg cgagcaacga gctccttgcc ggtgcccgag tcgccggtga
     6901 tcagcacggt cgcatcggag ttcgcgacgc gatggaccat ctcgatggtc gtgcgaatcg
     6961 ccgggctgtt gccgacaatt ccatccgtca tgaacgcatc gagcgcttgg ctatgcagct
     7021 tcttcagctc gcggcgcagc gcgaggtggt cgagggcgcg ggcaatcgcg atcgcgagca
     7081 cgtcgacctt caccggcttc tgaatgaagt cgaacgcgcc ggcgcgcatg gcaccaatcg
     7141 cgacgtccag gttaccgacg ccggtaatca cgatgggcag ggtctccatg ccggtcgcac
     7201 gaatctcctg acagagctcg atgccggaca taccgggcat ctggacgtcg gtcacgacca
     7261 catcggctgg ttgccgggtg aagcgcgcga gcgcctccat gccggaccgc gccgcctcga
     7321 catcccagcc tctcttcgcg agtgcatcgc gcagaagctc tgcggtgtct tgatcgtcat
     7381 cgacgacgag aattttgcct tgcttcgtgc ccatgttcgg ttccccgcct aatgcgaaga
     7441 caatgcccgc ggcactgtcg catgacggcg cgcccagagt gtggcggatc gacgcgtcga
     7501 accgcatagt tggcgaaatt tacgcagcta cttgctcctg cctagctgta gagccagcgc
     7561 tcaccgagcc aaatagctcg tggctattca caggagttag gtcttgccta tctcaccgca
     7621 acggcaccga aacgcacaac ggcccgccga agcggaccgt tcctgcagcc ggcctggagt
     7681 ggcttagacc ttcggacctg cgccgcggac ttcggtagag gcctgcgcct cgtacttctt
     7741 gaagttctct ttgaacagcg aggcgacctt ctttgccttc tcgtcgtacg cggccttgtc
     7801 agcccacgtg ttgcgcggct gcagcatctc ggacgggcac tccgggcagc tctgcgggac
     7861 cgcaaagccg aagatcggat cctcgaccgt cgggacgttc gagagcgcgc ccgagtggat
     7921 cgcgtcgatg atcgcgcgcg tgtacttcag cttgatgcgc gagccgacgc cgtacgcgcc
     7981 gcccgaccag cccgtgttga cgagccacgt ctgcgcgccg tgcttgcgga gcttctcggc
     8041 gagcatctcg gcgtacttgg tggggtgcca cacgaggaac ggtccgccga agcatggcga
     8101 gaacgtcggc tccggatcct tcacgccgac ttcggtgccg gcgaccttgg ccgtgtagcc
     8161 cgagatgtag tggtacatcg cctgcgcagg cgtcagcttc gagacgggcg ggagcacgcc
     8221 gaacgcgtcg gccgtgagga agatgacgtt cttcggatgg ccgccgaccg ccgggagctt
     8281 cgcgttcggg atcgtgtcga ggatgtagct gccgcgcgtg ttctcggtga tcgaggtgtc
     8341 gtcgaagttg acgacgcgcg tgtacggatc cagaacgacg ttctcgagca cggcgccgaa
     8401 ttggagcgcg ttccagatgt cgggctcgct ctccttggag agcttgatcg ctttggcgta
     8461 acagccgcct tcgatgttga agacgccgtc gtccgtccag cagtgctcgt cgtcgccgat
     8521 gagcaggcgc ttcggatcgg ccgagagcgt cgtcttgccg gtgcccgaga ggccgaacag
     8581 gatcgacgtg tcggagccgt cgcgagcctg cgtcgcggag cagtgcatcg acagcacgcc
     8641 ctgcttcggc atcaggtagt tcatgatcgt gaacacgccc ttcttcatct cgccggcgta
     8701 ttcactgccg aggatgacga actcgcggcg gccgaacgag aggtcgatcg acgtcgacga
     8761 cgtcatgcct tgcgtgtggc ggttcgccgg gaaaccaccg gcgttgtaga tgacgtagtc
     8821 gggctcgccg aacgactcga gctgcttgtc cgtcggacgg atgagcatgt tgtgcatgaa
     8881 caacgcgtgg taggcgcgcg cgcagatcac gcgaatcttg agctggtact tctcgtccca
     8941 accggcgtag ccgtcgacga cgtaaaggcg gtcacgcgtg ttgaggtaat cgatcgcgcg
     9001 ctcgcggttg atcacgaacg actgcggctc gagcttgacg ttgacgttgc cccaccacac
     9061 ctcgtcgcga acttctggct cgtcgacaac gcgcttgtct gtgggcgagc ggcccgtctt
     9121 cttccccgag tacgccacca tcgcgccagt cgacgagatc gcggtgccct tttcgaactt
     9181 cagcgctgct tcgtagagaa atgccggcgt cgcattgcga tgaatttcag cgacggagat
     9241 gccttgtttt tcgagggaga aagccgccat gcggggtttt atacactgaa tcagggatta
     9301 gcggtccccg gcgtgaggga cgtgcagaac ttccagtccg ccgatgcgct gtcggtgtcg
     9361 cgaccgttag gtgaccggca gagagagccg agcaccgtgt tcgagtccgc gatcgacgtg
     9421 ctaagtgcag tgccttcgac gagcgaaacc ggattcgcaa tgcccggtac attcgcggcc
     9481 gtgacggagc cctcatagct cagtgcgtcg agcagcgtgt tggtcgaggt atccacgagt
     9541 gcgacgccgt cggggttgcc gttctggatg ccgtccgtgg tccaacccgg ctcgaacttg
     9601 agcgccggcg gagcgaccgc gagcgcttgt cctgcgacga cgaggtagcc gcccgcggga
     9661 atcgagcccg cgctcgatag atcaatgatg gagtccgcgt tcggatacgc gtcaccggtg
     9721 gatccattga tgagcaccag cgcgacgttc gcgagactga ccgccgagct cgtcgggttg
     9781 taaatctcga tgtactccgc gttgtcgttc ggcgggaccg cgcccgtgtt gtcgtagtcg
     9841 acctcgttga tcacgaggtg gctcgcgccc gcgttgacgg tgaccgtcgc gtttgcagtg
     9901 ttgccgccgg agagcgcagc gctcacagtc gcgttcgcgc cggtcgcgtc gccgtagaca
     9961 aacgtgttcg atagcgtgtc cgccgcgatc acgagcgtcg tcgccggatt cgtcgagccc
    10021 gacgtgcccg gagagactcc gagcgtgagc gacgtgccgc ctggaggcgc cggaatgtcg
    10081 agcgtcgcgg tgaacgtcgc cgagccgagc ggagcgaccg cggtgctcgt cggagcgatc
    10141 gataccgacg tcggtgcttc gttcgcgccg aggacgcgga cttgcgcggt cttcgccgct
    10201 ccaccgccga gctgtgcagt gagcgtgatc ggcgtcgtgt tttggttcac gccgtcgagg
    10261 acaacctttg cggtcgtctc gccattcagg accgtcacct tgccaccgac gacgacgaca
    10321 tcaccgctcg gcgacgagac cgtcacgtcg gtgttgccct gcgcgggacc cgagagcgtg
    10381 accgtcagct ccatgccggc ggggatcgaa ttgacgccgc tctggcccgc gcgcacgaag
    10441 ccaagcgcag gaccaaacgc agcgagcgtg ggcgtgcctt gcacgagatc cgcggcgctg
    10501 cgcggctcga gcttcgatgc ttgctggcgc agcgcgagga taccgatgac cgtcgagtac
    10561 gacgttccga ccgcgctgcc gttcgcgggc gtgacataca ggaagtcgtc gacgatgagc
    10621 gtgtcggagc cggccgtcag cgtgtactcg ccgaacgtcg cgttgagcgc cgatacggtc
    10681 gccgcgccga cggagatgag caccgcttcg tacgccgctg ccttcgggcc gcccgtcttc
    10741 acgtcggcat acgtggtcgc cacaggagtt ggtgccgcct cgccgaccat cgtcgtcggc
    10801 gtgaccgatg cgacggtgtc gagctcgagc tgattctgga agttcgtgac cttgccgtcg
    10861 acggtgacgc gcagaccgac cgtcgctttc gcgagcgttg gcgccatcgc acccgtgtag
    10921 acgaagatgc cggagaagtc ggcgcccatg tagccggcgt cgcccgcctt cgtctgcatg
    10981 aagaagccgt tcgtgccttt gccggtgacg agcgcgttct cgatgcgcac ggtcgttccg
    11041 gtggagacct cgcccttctt gatcgcgtag atcgtcgcgg ggcagcccgc cgttcccggg
    11101 ttcgcattgc tcgggcacgc atcgcatgcg tcgccgtgac cgtccatgtc gccgtcgctc
    11161 tggcccgggt tcgcggtgtc gggacagttg tcggcgctgt tgatgaagcc gtcgtgatcg
    11221 cgatcgtcgg gattgaccgg cgtgcacgcg gtcgtgttcg catcggtcgg gcaggggtcg
    11281 cacgcgtcgc ccgccatgtc gttgtcggtg tcgggctgct tgccgccgtc catcggacga
    11341 atcgggttga agaccttcgg gcagttgtcc gcggtgtcgg gcagaccgtc gccatcgctg
    11401 tcgtcagctg aaatcgcgcc cgtgtagatc gtcgagccgc tcaccgctgc ggggcgcttc
    11461 ggcgtgcacg acggctcctt gtcgggaacc gcgcagaaga acgccggata gatcgtgccg
    11521 acggcagcct gcacttcggc gagcgtcttg ccgatttcac tcttcaagca gacgcgcttg
    11581 ccggtgccgc acacgtcgat cgtgtcgcag tcactcgaga gcgccgcgat cgacgcatcg
    11641 tcgccgaaca gcgccttgcc accgcgcatc acgagcacga cgtcctgcgg atcggcatcg
    11701 atgatcgagc ggaacgggcc gccggccttg cccttgaaga tcgagatgtc ggcgacgtga
    11761 ttcggcgcga gcaggccgat gacgtcgtcg gtcgcggtga ccgccgcagc gttgagcgtg
    11821 gtcatctccc acagctgctt gtcggtgaag tagttgtcgt aatacgtctt gttcaagctg
    11881 tcggcgcagc gcagctcgcg cagcatgttc atcgagcccg tcggcgtcca atcggtgccg
    11941 agtgcgatct cgacgccgaa ccgatgcgcc gtggtgacgc gcgcggtgtc gccatagagc
    12001 gtgatgttcg agcgcggcga ccagatcagt gccgtgttct tgagcgccat cgcgccgtaa
    12061 ttgtcggcgg tgagcccgat cgcgtgaatc atcgccgtct tcggcagcag cagatcgttc
    12121 gagatcttcg tgccggtgac gtcgtacgtc gtcgagctct cgcagcggaa ttcgttctgc
    12181 gcgtagaggt cgatgccctc ggcggtgtgc ggctcgaacg cgtcgacatt cgcgatcgat
    12241 gcagcggtca cggcggtgcc gtagtcgcag tccatcgtgc gccgtgtgcc cgacgtatcg
    12301 ccgagaggga acgtctcgaa ctcgacggcc ttctgattca agccctcttg tccggtcgcg
    12361 ttgtcgaggt tgcaaagagg gcggaatcgc gggcatggtc gtcgcagatg gcgatggctt
    12421 gccgctcgcg gctgccggtg atgtgtcatc gcgctgtcgg gctgcacttc atctgcggca
    12481 gcagc
//
LOCUS       DATLBZ010000209         3165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3935216, whole genome shotgun sequence.
ACCESSION   DATLBZ010000209 DATLBZ010000000
VERSION     DATLBZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3935216"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1991)
                     /locus_tag="VMZ53_11010"
     CDS             complement(<1..1991)
                     /locus_tag="VMZ53_11010"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29036.1"
                     /translation="MKTIKSIAFGAALFAAGAGVGCGSDDNTGPLGSVDSLVILQRPK
                     RNDMGDIFQYTSYIPGARLVKLSPPTADGKLEVLCCDKAGAEFAAIDISSYDLSFDAK
                     EIVFAGKLSANERYGLFRLKLETGAVEQLATDPGRDYVNPIYLPGDKVMFTTNAVVEP
                     GAPQHRDEYERGVTLQVGTINIDGTGEVLGPRNLSHRTFPSLASDGRIMLTQWDHLGP
                     VNAGHLMFMNTDMTELREAFGKEGTGASNSTLKAREVSPGRFVAIATSRDRTIQAGKL
                     VDIRLGVPSTKDGLVRADELMSEANASYVDLTPDVPMDREPSAMTIGRYYDAYPLNAK
                     EKLDLLVSWADGPVESGVLGQAQTSANFGVYLYDSTKLTRHPILDDPAMWDIFARPLQ
                     PRTAPPVTGSSIEASLGGSALVGSMNVYQSSLKTFVPGSVYGVRIMEGFSSEEGFPEM
                     FGTTMFEGQANLGVARVASDGSWLATVPANVPLHLQAVDNFGMSLENEPVWFSARAGE
                     ARVCGGCHENRAETTVINPGITQAAAIGPMAAMSAVPRLQRLSTDSQLASPTTPPDQL
                     VGMAWNKAVQPVFDAKCVSCHDSNNTAGIQGYTITDPTTGAVVSQWTFNLSNTPVTID
                     YGMGMMSTFSASYLTMAGPDMEAIEKNGLVVTGNFKVYM"
     gene            complement(2129..>3165)
                     /locus_tag="VMZ53_11015"
     CDS             complement(2129..>3165)
                     /locus_tag="VMZ53_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29037.1"
                     /translation="AREAELFRQEVLAVAEAAPKISAAGAALAVLDACASLAEVAAKR
                     SYCRPVVDDGLALELGDARHPVIEAMLPPGSFVPNDCRLDPAAEQLVLITGPNMAGKS
                     TYMRQVAQIALLAQIGSFVPAKSATIGICDRVFTRVGAADNLSRGDSTFMVEMRETAA
                     ILAKATKRSLVILDEVGRGTSTFDGVSIAWAVAEYIHDQIGARTLFATHYHELVALAD
                     SRPRVRNVYVAVREHKSEIVFLRRVVPGGANKSYGIDVARLAGLPKHVVARSREIMSK
                     LEGGGALAASPQLSLIAAPGNRISSLGDNRSGSIAEPSPALARIAAIDIDRTTPLEAL
                     QLLSELKKLV"
BASE COUNT          505 a         1044 c         1071 g          545 t
ORIGIN      
        1 ttcatgtaga ccttgaagtt gccggtcacg acgaggccgt tcttctcgat ggcttccatg
       61 tcggggccag ccatcgtgag gtacgaggcc gagaacgtcg acatcatgcc catgccgtag
      121 tcgatcgtga cgggcgtgtt cgacaggttg aaggtccact gcgagacgac cgcgcccgtc
      181 gtcgggtccg tgatcgtgta gccctggatg ccagccgtgt tgttcgagtc atggcagctg
      241 acgcacttcg catcgaacac cggctgcacc gccttgttcc acgccatacc gacgagctgg
      301 tcgggcggcg tcgtcgggct cgcgagctgg ctgtccgtcg acaggcgctg caggcgcggc
      361 acggcgctca tcgcagccat cgggccgatc gcagcggctt gcgtgatacc ggggttgatg
      421 acggtcgtct cagcgcggtt ctcgtggcaa ccaccgcaga cgcgcgcttc accggcgcga
      481 gccgagaacc acacgggctc gttctcgagc gacatgccga agttgtcgac ggcctggagg
      541 tggagcggga cgttcgccgg caccgtggcg agccagctgc cgtccgacgc gacgcgagcg
      601 acgccgaggt ttgcttggcc ttcgaacatc gtggtgccga acatctcggg gaagccttct
      661 tccgacgaga agccttccat gatgcggacg ccgtacaccg agccgggcac gaacgtcttg
      721 aggctcgact ggtagacgtt catcgagccg acgagcgccg agccaccgag gctcgcttcg
      781 atcgagctac ccgtaacggg cggcgccgta cgcggctgca gcgggcgcgc gaagatgtcc
      841 cacatcgccg gatcgtcgag gatcgggtga cgcgtgagct tcgtcgagtc gtagaggtag
      901 acgccgaagt tggccgacgt ctgcgcctga ccgagcacgc ccgactcgac ggggccgtcc
      961 gcccacgaca cgagcagatc gagcttctcc ttcgcattca gcggatacgc gtcgtagtag
     1021 cgaccgatcg tcatcgcgct cggctcgcgg tccatcggaa cgtcgggcgt caagtcgacg
     1081 tagctcgcgt tcgcttcaga catcagctca tccgcgcgga cgagaccgtc tttggtggac
     1141 ggaacgccga ggcggatgtc gacgagcttg ccggcttgaa tcgtgcggtc acgcgacgtc
     1201 gcgatcgcaa cgaagcggcc cgggctgacc tcgcgcgcct tgagcgtcga gttcgaagcg
     1261 ccggtgcctt ccttgccgaa tgcttcgcgc agctccgtca tgtccgtgtt catgaacatg
     1321 aggtggccgg cgttgacggg accgaggtgg tcccactgcg tcagcatgat gcggccatcg
     1381 ctcgcgagcg acgggaacgt gcggtgcgag aggttgcgcg ggccgagaac ctcgcccgta
     1441 ccatcgatgt tgatggtgcc gacctggagc gtcacgccgc gctcgtactc gtcgcggtgc
     1501 tgcggtgcgc ccggctcgac gacagcgttg gtcgtaaaca tgaccttgtc gccgggcaga
     1561 tagatcgggt tgacgtagtc gcggcccggg tcggtcgcga gttgttcgac ggcgcccgtc
     1621 tcgagcttga gacggaacag gccgtagcgc tcgttcgccg agagcttgcc ggcgaagaca
     1681 atctccttcg cgtcgaacga gaggtcgtac gacgagatgt cgatcgctgc gaactcggcg
     1741 ccggccttgt cgcagcagag gacttcgagc tttccatcag ccgtcggcgg cgagagcttc
     1801 accagccgcg cgcccgggat ataggacgtg tactggaaga tgtcgcccat atcgttgcgc
     1861 ttcgggcgct gcaggatcac gagcgagtcc acgctgccga gtggccccgt gttgtcgtcg
     1921 cttccgcagc ccacgcccgc ccccgcagcg aacagcgcgg caccgaacgc gatgctcttg
     1981 atggtcttca tgccggcctc taactacaag gcccaggcca gctccaccgg ctcggaatca
     2041 ttgcgttcgt cgtcgccgcg gctcggggcg cctgccccct atcctgcccc cttgggggtg
     2101 acgagccaca acgcgatgta ggcgtacgtc agacgagctt cttgagctcg gagagcagct
     2161 gcagggcttc gagcggcgtc gttcgatcga tgtcgatcgc ggcgatccgc gcgagcgcgg
     2221 gcgacggttc ggcgatgctg ccgctgcgat tgtcgccgag cgacgagatg cgattgccgg
     2281 gcgcagcgat cagcgacagc tgcggcgagg ctgcgagcgc gccgccgccc tcgagcttgc
     2341 tcatgatctc gcgcgaccgc gcgacgacgt gcttcggcag gccggcaagc cgcgcgacgt
     2401 cgatgccgta gctcttgttc gcgccgcccg gcacgacgcg ccgcaggaac acgatctcgc
     2461 tcttgtgctc gcgcacggcg acgtacacgt tgcgcacgcg cgggcggctg tccgcgagcg
     2521 cgacgagctc gtgatagtgc gtcgcgaaca gcgtgcgcgc gccgatttga tcgtggatgt
     2581 attcggcgac cgcccacgcg atcgacacgc cgtcgaacgt cgacgtgccg cggccgacct
     2641 cgtcgaggat cacgagcgat cgcttcgtcg cctttgcgag gatcgcggcg gtctcccgca
     2701 tctcgaccat gaatgtcgaa tcgccgcgcg agagattgtc cgccgcaccg acgcgcgtga
     2761 acacgcgatc gcaaattccg atcgtcgctg acttcgccgg cacgaacgag ccgatttgcg
     2821 cgagcagcgc gatctgcgcg acctggcgca tgtacgtcga cttgcccgcc atgttcggcc
     2881 ccgtgatgag cacgagctgc tcggccgcgg gatcgagccg gcagtcgttc ggcacgaagc
     2941 tgcccggcgg aagcatcgct tcgatcacgg gatgccgcgc atcaccgagc tcgagcgcca
     3001 agccatcgtc gacgacgggc cggcagtagc tccgcttggc cgcgacctcc gcgagcgacg
     3061 cgcatgcatc gaggaccgcg agcgccgcgc ctgccgctga aatcttcggc gccgcttctg
     3121 cgacggcgag cacctcttgg cggaacagct cggcctcgcg cgcga
//
LOCUS       DATLBZ010000210        10922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_956570, whole genome shotgun sequence.
ACCESSION   DATLBZ010000210 DATLBZ010000000
VERSION     DATLBZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956570"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VMZ53_11020"
     CDS             complement(<1..738)
                     /locus_tag="VMZ53_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HUS29038.1"
                     /translation="MSSDPKNATALKRGLGRGLSALLPTAAAPSPARIEHAPSRRTYF
                     SAQIEEVYPGPEQPRHNFDEGELSELADSIKVHGVIVPLVVRPRQGGGYFLIAGERRW
                     RAAQRAGLHEVPVVVQEVDSQVAFERALVENLQRSDLGPIEEATAFQRLIDEFRLTQD
                     QVAERIGKDRSTVSNAIRLLKLPTAVRQMVEDDRLSMGHARALLGLEDPVAIERTART
                     VVDKGLSVRATENLVKQDRAPEKAKPRI"
     gene            complement(738..1547)
                     /locus_tag="VMZ53_11025"
     CDS             complement(738..1547)
                     /locus_tag="VMZ53_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS29039.1"
                     /translation="MSRIVAVANQKGGVGKTTTSINLAASIASRGYRVLLVDFDPQGN
                     ASSGVGYPRDKVELTVYDALVGECTLEDVIRPTDIPTLFVAPATTDLVGAEIELIGAE
                     RRERFLADALAPIAAQYDYIVIDCPPSLGLLTLNALVAADGVVVPMQAEYFALEGLSA
                     LTATIQKVKEAYNPPLTIDGVIFTMFDGRMNLANQVRAEVAKHFGDKVLETTVPRNVR
                     LSEAPSHGKPVMLYDIRCPGAKSYLAVADEFIERTIVERAPKGQPAVGSQS"
     gene            1661..1846
                     /locus_tag="VMZ53_11030"
     CDS             1661..1846
                     /locus_tag="VMZ53_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29040.1"
                     /translation="MSSGIPRIYTSFAEFEREELRRLDSLHTSVDDLVEERFAEDLDF
                     GQSDAPRKRGRPRKNPR"
     gene            complement(1854..2939)
                     /locus_tag="VMZ53_11035"
     CDS             complement(1854..2939)
                     /locus_tag="VMZ53_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29041.1"
                     /translation="MGPFTGTTHRYVVDSIELPKTNTQAREFGDNLDGLRGVDNQLGM
                     VVSTISSQGLGTTHGPDMIAAGALSSSFEIVADDLANDDTVSVIYRGNDASGGQPVGG
                     RIVDGWFRSNRTSTTEHPGRAFARLPVFADADPSEIVISNMEIDLQPDGAGGFTGFVR
                     GTTDAEDTTSEAYRGWMQMLAADPDGHLTAMSLFDEPPRDWTITEYEFTHNSLIQSLL
                     LPDIELNGEDVLSFGFRVHIKPCDAGTCLTTPPADACHDRVIDGDETDVDCGGGCLAC
                     AAAATCEVAADCQSGTCAAGVCTASSCSDGLRNGFETDVDCGSACGATCQVGERCHSD
                     GDCAAGHCGPDCTDPLTCDYPYFDTCR"
     gene            complement(3157..3741)
                     /locus_tag="VMZ53_11040"
     CDS             complement(3157..3741)
                     /locus_tag="VMZ53_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29042.1"
                     /translation="MFWFIGGGFVLTTAAFAIAFMRSQRGPKEPIVPIASAPDGVRLR
                     VLGKVVLDGEPLRAPWTGRACVYYRLELRVFSGQARTVDTVAGACDFAVRDDSGQAQV
                     LSEHATFDLEADIVEAAPAASLSEKSRDLIREHGWNVPEVAQVELSEAALEVGATINV
                     VGAPTRETTLVFARDHEVIEKPRTRRFIGKRWRD"
     gene            complement(3741..5636)
                     /locus_tag="VMZ53_11045"
     CDS             complement(3741..5636)
                     /locus_tag="VMZ53_11045"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS29043.1"
                     /translation="MSLARLVVIEGPDAGREIELRMRGGKIGRGDDCEYKLTDPTVSR
                     NHGLIEMRDGALTFVSEKRTLINGAEATHHRLESGDEIVIGATKLAYLPEEGVAITRQ
                     ASNVTMEVSSRQLLALTGRDDRARRHLAAIAQLGDRLRAEAAAGRDAVARAACDAART
                     ALEAHRAFVLSAGKRPTPVAASIGPQESSQLQISGDIVDKVRDQRQVVTGESGGRALI
                     AAPVHGGADEVVAILWVDRRGAPYEEADSLAAGCLAHLTGAAWVGADTREQLTRRADA
                     LEERLADGDFVGKSAAAQRVIDFVNRVGPSDATILLGGESGSGKEMVARAIHRASRRA
                     KGPCVAVNCAALTESLIESELFGHEKGAFTGATEKKAGRFEMADRGTLFLDEVGELPL
                     GLQTKFLRVLEERRFERVGGQKAIEVDVRVVAATNRDLQEMVKRGQFREDLYYRLSVI
                     HIDVPPLRERLDDVPLLAEHFLARYRQQAARRISGFAPDAVAAMTRYSWPGNVRELRN
                     AVERAIVLGDREQIIAQDLPPQVLAQGSLPRARNSPPTPPLGSAMSTAVPSVSSAPTP
                     APIPQVASHAVIDAPPRARSLRELEKEGILAALAATGGNKAQAAAILEIDRSTLYKKL
                     KDYDIEG"
     gene            5666..6127
                     /locus_tag="VMZ53_11050"
     CDS             5666..6127
                     /locus_tag="VMZ53_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29044.1"
                     /translation="MKRSAIAFLAMASLAACSGKSKNGSTTDGEGSGSAAIYAKKYVV
                     SFGMTQAASSAEIFLQTTDETGKQISHPLGTFDGQCQVIKPAEDMKAVTGVSCTQTGS
                     DHGIELDAVIIGGEIVVLKLETHVGVAPDPMARTEVTRIKAPVGSAVSPEG"
     gene            6683..7354
                     /locus_tag="VMZ53_11055"
     CDS             6683..7354
                     /locus_tag="VMZ53_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29045.1"
                     /translation="MPLTFWTCMDAGGTNLVHRAELGPVLYVNSCAPGYLPLLRESST
                     VSTTICVAVCKPLNCYAGNCGTNAENRLGAAPHRCMNPDAAGTFDTGPNGEQCEFVWR
                     REIDPMAGTYLPSTTSDTLGFCFDHSKYLYAANGGGPDTPLPACSDLADGFGSGSDPS
                     SPLTYWGAADLGCVDSTHAGVHAATGKPLRRSPLDDLRPFFGSQHLSSARSEIRTTGR
                     NWQRP"
     gene            7473..7793
                     /locus_tag="VMZ53_11060"
     CDS             7473..7793
                     /locus_tag="VMZ53_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29046.1"
                     /translation="MVLASGCVAGGDEPTATIEIKTDTTTAVTIDHPVAELRDAVSEA
                     SVPDVCSLAAELPTSDVCSLICDPPAMAQYLIDEGMHGGTCYQLRCVLPGRENAVMVG
                     VCLL"
     gene            7843..8352
                     /locus_tag="VMZ53_11065"
     CDS             7843..8352
                     /locus_tag="VMZ53_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29047.1"
                     /translation="MKRLGMVAAALVGCTNGGSPASPDAAGVQPDAPALAPAVPGACH
                     ESITLYGLDGTNGRKTVGPLALDTQGVTLCLTLDATQNIVVGHFGADSGREQAAASTF
                     AMSLFDANGTLLRDGWDVTFGSSPPTTFANLEYGVTKGTILEAKLVIRTRAGQASTSV
                     GLDLIEPYE"
     gene            complement(8354..8959)
                     /locus_tag="VMZ53_11070"
     CDS             complement(8354..8959)
                     /locus_tag="VMZ53_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS29048.1"
                     /translation="MWRVLVLAVSLTGCGRLFGLEPPTLDDGTVDEDASVSDASSDAR
                     IADAPADTPSNALCPQSYSMVLGTSPSRYRFVNGGTAWDVAQAACLADQITGSTRFTH
                     LIVAANDAELSALDAALGGEKWVGLSDRAIEGAYQWVTAETNNYPPSTGGPWGTDEPK
                     ASGAEDCVLMSNTGDLEMRSCGLGRQYVCECDAFPNDPTKY"
     gene            complement(8972..9583)
                     /locus_tag="VMZ53_11075"
     CDS             complement(8972..9583)
                     /locus_tag="VMZ53_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29049.1"
                     /translation="MTTATAFSSSRSSRTLPTYKASAFPSEAALRLEQTPMSGLEKVF
                     VRGATPDLDSLVGWEFRGINHLPLNAIPLAQLAGIKKFVKGFHRREDGKVMGYNSPVK
                     NNVLDGRWNVGAKRFGFYETDHVDATSRDNAYLHAVLLDYGKADNGLNPMSQLRDYLV
                     QVDPANPDLFLGKAYAAVGPLRIPLNFFILERFRIGLTDYATR"
     gene            complement(9580..>10922)
                     /locus_tag="VMZ53_11080"
     CDS             complement(9580..>10922)
                     /locus_tag="VMZ53_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HUS29050.1"
                     /translation="TSAMVRQLYRDGGATMALGNPPILFQEGRAVGGSTVINGGMSWR
                     TPEDILARWRNEAGLSITPKELEPYYERVEKRINVAQNDEAAIGRDNLLLKKGADAMG
                     WKTVSNLRNQVHCAGSNRCPFGCPTGAKQSALVSYIPRALHFGARVYADVKVDRLTFH
                     GKRATGVIGRVQREGGGESWRVVVRAKLVVASCGAIHTPALLRRSGLESRSGMLGGNL
                     TLHPNVKVVPIFDEDVTSWKGVHQAFQVREFVDQGIGTFAAINVPPSIVAMSLPHKGK
                     QLGEIMQQYNNMFVAGLLMEDSTTGRVRTINGRPQAFYQLVDRDIALMQKGLTLLSEL
                     LFAAGAKKVLLPFHHASEMLSADDTRKFFSKPIPASAWEVVTVHMMGTCSMGTDRLKA
                     VTDEYGFMHDVDRLLISDASLFPTPIRVNPMETIMTLATRAAGYVIDNSRRLLS"
BASE COUNT         1820 a         3567 c         3601 g         1934 t
ORIGIN      
        1 gatgcggggc ttggcttttt cgggggcgcg gtcctgcttg acgaggttct ccgtcgcgcg
       61 gaccgacagg cctttatcga cgaccgtgcg cgcggtgcgc tcgatcgcga ctggatcttc
      121 caggccgagg agggcgcgcg catggcccat cgataggcgg tcgtcttcga ccatctggcg
      181 cacggcggtc ggtagcttca gcagacggat cgcgttcgat accgtgctgc ggtctttgcc
      241 gattcgctcg gcgacttggt cctgcgtcag gcggaactcg tcgatcaggc gctgaaacgc
      301 cgttgcctct tcgatcgggc cgaggtcgct gcgctgcagg ttctcgacga gcgcgcgctc
      361 gaacgcgacc tgcgagtcga cctcttgaac gacgaccggc acctcgtgca gccccgcacg
      421 ttgcgccgcg cgccaccgac gctcgcccgc aatcaggaag tagccgccgc cttggcgagg
      481 acggacgacg agcggaacga tgacgccgtg cactttgatc gaatcagcga gctcggagag
      541 ctccccttcg tcgaagttgt ggcgcggctg ctcggggcct gggtagacct cttcgatctg
      601 cgcgctgaag tacgtgcgcc ggctcggcgc gtgctcgatg cgtgcggggc tcggggccgc
      661 cgcggtgggc agcagcgccg acaggccgcg accgaggccg cgcttcaatg ccgtggcgtt
      721 ctttggatcc gagctcatca tgattggctc ccgacggcag gttgcccttt cggtgcgcgc
      781 tcgacaatcg tccgttcgat gaactcgtcg gcgacggcga ggtagctctt cgcaccgggg
      841 cagcggatgt cgtagagcat gacgggcttg ccgtggctcg gcgcttcgga gaggcgcacg
      901 tttcgcggca ccgtcgtctc gaggactttg tcgccgaaat gcttggcgac ctcggcgcgg
      961 acttggttcg cgaggttcat gcggccgtcg aacatcgtga agatgacgcc gtcgatggtg
     1021 agcggcgggt tgtacgcctc tttcaccttt tggatcgtcg cggtgagcgc ggacaaacct
     1081 tcgagcgcga agtattccgc ctgcatcggc acgacaacgc cgtcggccgc gacgagcgca
     1141 ttgagcgtca gaaggccgag cgacggcggg caatcgatca cgatgtagtc gtactgcgct
     1201 gcgatcgggg cgagcgcatc ggcgagaaac cgttcgcgcc gctctgcacc gatcagctcg
     1261 atctctgcac cgacgagatc cgtcgttgcg ggcgcgacga acagcgtcgg gatatccgtc
     1321 ggacggatga catcctcgag cgtgcattca ccgacgagcg cgtcgtagac cgtcagctcg
     1381 actttatcgc gcggatagcc aacaccgctc gacgcgttgc cttgcgggtc gaagtcgacg
     1441 agcaatacgc gatagccgcg tgacgcgatc gacgcagcga gattgatcga ggtcgtcgtc
     1501 ttgccgacgc cccccttctg attcgcgact gcgacgatgc gggacatgca acccgggtaa
     1561 cacgcagcta tgacgatcca aaaatttggc cccgagatcg cgctcagcgt accttgtact
     1621 cagatgtagg acatgtgggc atgtcctcga aggagcgcca atgtcgtctg gtattccgcg
     1681 tatttatact tcgtttgccg agttcgagcg tgaagagctt cgccgtctcg attcgctgca
     1741 cacctccgtc gacgatctcg tcgaagagcg cttcgccgag gatctcgact tcggtcagag
     1801 cgatgcgccg cgcaagcgtg gccgtccgcg caagaacccg cgctagtcgc tcgctaccgg
     1861 cacgtgtcga agtacggata gtcgcacgtg agcggatcgg tgcagtcggg gccgcagtgg
     1921 cccgccgcgc agtcgccgtc ggagtggcag cgttcaccga cctgacaggt cgcgccgcac
     1981 gcactgccgc agtcgacatc ggtctcgaag ccgttgcgaa gaccgtcgct acacgacgac
     2041 gcggtgcaca cgccagcagc gcacgttccg ctctggcaat cggctgcaac ctcgcacgtc
     2101 gcggccgccg cgcacgccag acaaccgccg ccgcagtcga cgtcggtctc gtcgccgtcg
     2161 atcactcgat cgtggcacgc gtccgcgggc ggcgtcgtca ggcacgtgcc ggcgtcgcac
     2221 ggcttgatgt gcacgcgaaa gccaaaggac aggacgtcct ctccgttcag ctcgatatcg
     2281 gggagaagca gcgattggat caacgagttg tgagtgaact cgtattcggt aatcgtccag
     2341 tcgcgcggcg gctcgtcgaa tagcgacatc gcagtgagat gaccgtcggg atcggcggcc
     2401 aacatctgca tccaaccgcg gtacgcctct gacgtcgtgt cctcggcatc cgtcgtcccg
     2461 cgaacgaaac cggtaaaccc gccggcgccg tcgggctgca gatcgatctc catgttcgag
     2521 atcacgatct ctgatgggtc ggcgtcggcg aacacgggca gtcgagcgaa cgcacgtccc
     2581 ggatgttctg tcgtcgacgt gcggttgctg cgaaaccaac catccacgat gcgcccgccg
     2641 acgggctgac ctcccgacgc gtcgttgccg cgatagatca cggagaccgt gtcgtcgttt
     2701 gccagatcat ccgcgacgat ctcgaagctc gacgagagcg cgcccgctgc gatcatgtcg
     2761 gggccatgcg tcgtgccgag gccctgcgac gagatcgtcg agacgaccat gccgagctgg
     2821 ttatcgacgc cgcgcagacc gtcgaggttg tcgccgaatt ctcgcgcctg cgtgttcgtc
     2881 ttcgggagct cgatggaatc gacgacgtag cgatgcgttg tccccgtgaa gggccccacg
     2941 acgtcgtcag atacggggtc ggatgcacag gcggcaaaga tcagaacgaa gggagcgaat
     3001 ctcatgacgg cgcggctctt tgcagcgaag gtgccaaggt ccgaccactg aattcgcgcg
     3061 agttccgcac gtcgcatcgc cgaaactcgc gagaggcacg acgcggatgt cgtgacctcg
     3121 acgccctgtt gaaagtctgt cagcgatcga gcgtcttcaa tcgcgccatc gcttgccgat
     3181 aaagcgacgc gttcgcggct tctcgatgac ttcatgatct cgcgcaaaca cgagggtcgt
     3241 ttcgcgggtc ggggccccga ccacgttgat cgtcgcgccg acctcgagcg cggcttcgct
     3301 gagctcgacc tgcgcgacct cgggaacgtt ccagccatgc tcgcggatga ggtcgcgcga
     3361 cttctcgctc aatgaagcgg caggcgcagc ctccacgatg tcggcttcga ggtcgaacgt
     3421 cgcgtgctcg ctgagcacct gcgcctgacc gctgtcatcg cgcaccgcga agtcacaggc
     3481 gcccgcgacg gtatccaccg tccgcgcttg gcccgagaac acgcgcagct cgaggcggta
     3541 atagacgcag gcgcgtccgg tccacggtgc gcgcagcggt tcaccgtcga gcacgacctt
     3601 gccgagcacg cgaagccgga ctccgtcggg cgcactcgcg atcggcacga tcggttcctt
     3661 cggcccgcgc tggctgcgca tgaacgcaat cgcgaacgcc gccgtcgtca ggacgaagcc
     3721 acctccgatg aaccagaaca tcagccttcg atgtcgtagt ctttgagctt cttgtagagc
     3781 gtcgagcgat cgatctcgag gatcgcggcc gcttgcgcct tgttgccgcc cgtcgcagca
     3841 agcgcagcga ggatgccttc cttctcgagc tcgcgcaggc tccgtgcgcg cggcggtgca
     3901 tcgatcaccg cgtgcgacgc gacttgcggt atcggcgcgg gggtcggtgc cgacgacacc
     3961 gatggcaccg cggtcgacat cgcagagccg agcggcggcg tgggcggcga attccgcgcg
     4021 cgtggcagcg agccctgcgc caggacctgc ggcggcagat cttgcgcgat gatctgttcg
     4081 cgatcgccga gcacgatcgc gcgctcgacg gcgttgcgca gctcgcgcac gttgccgggc
     4141 cacgagtagc gcgtcatcgc cgcgaccgca tcgggcgcga agccggagat gcggcgagca
     4201 gcttgctgcc ggtagcgcgc gaggaagtgc tcggcgagca acggcacgtc gtccaggcgc
     4261 tcgcgcagcg gcggtacgtc gatgtggatc acgctgagcc ggtagtagag gtcctcgcgg
     4321 aactggccgc gcttgaccat ctcttgcaga tcgcgattcg tcgccgcgac gacgcgcacg
     4381 tcgacctcga tcgccttttg cccgcctacg cgctcgaatc ggcgttcttc gagtacgcga
     4441 aggaacttcg tctgcagacc gagcggcagc tcgccgacct cgtcgaggaa cagcgttccg
     4501 cggtccgcca tctcgaagcg gccggccttc ttctcggtcg cgcccgtgaa tgcgcccttc
     4561 tcgtggccga acagctcgct ctcgatcagc gactccgtca gcgcggcgca gttgaccgca
     4621 acgcacgggc ctttcgcgcg acgtgaggcg cgatggatcg cgcgcgccac catctccttg
     4681 ccggagcctg actcgccgcc gagcaggatc gtcgcgtcac tcggcccgac gcggttcacg
     4741 aaatcgatca cgcgctgcgc cgctgcgctc ttgccgacga agtctccatc ggcgagccgc
     4801 tcttcgagcg catctgcgcg ccgcgtgagc tgctcgcgcg tgtcagcgcc gacccacgcc
     4861 gcaccggtga gatgcgcgag acatcccgcc gcgagactgt cggcttcttc atacggagcg
     4921 ccacgccgat cgacccacaa gatggcgacg acctcgtcgg caccgccgtg cacgggcgca
     4981 gcgatcaacg cgcgaccacc ggactcgccg gtgacgacct gacgctggtc gcgcactttg
     5041 tcgacgatgt cgcccgaaat ctgcagttgc gagctctcct gcgggccgat cgacgcagcg
     5101 accggcgtcg gccgcttgcc cgccgagagc acgaacgcgc ggtgcgcttc gagcgccgtc
     5161 ctcgcagcgt cgcaagcagc gcgcgccacg gcatcgcgac ccgccgcggc ttccgcacgc
     5221 aaccgatcgc cgagctgcgc gatcgcggcg aggtgccggc gtgctctgtc gtcgcggccg
     5281 gtcagcgcga gcagctggcg gctcgagacc tccatcgtca cgttgctcgc ctgccgcgta
     5341 atcgcgacgc cctcttcggg cagatacgcg agcttcgtcg cgccgatcac gatctcgtcg
     5401 ccggactcga gtcggtgatg cgtggcctcg gcgccattga tcagcgtcct cttctcggac
     5461 acgaacgtca gcgcgccatc gcgcatctcg atgaggccgt gattccgcga gaccgtcgga
     5521 tccgtcagct tgtactcgca gtcgtctccg cggccgatct tgccgccgcg catgcgcagc
     5581 tcgatctcgc ggcccgcatc cgggccttcg atcactacga ggcgcgccaa cgacatgcgc
     5641 gcatcttagt gtaagtttcg cgtcgatgaa gcgctccgcg atcgcgttcc tcgcaatggc
     5701 ctcgctggcc gcgtgcagcg gtaagagcaa gaacggctcg acgaccgacg gcgagggcag
     5761 cggctcggca gcgatctacg ccaagaaata cgtcgtctcg ttcggcatga cgcaggccgc
     5821 gtcctccgcc gagatcttcc tgcagacgac cgacgaaacg ggcaaacaga tcagtcatcc
     5881 gctcgggacc ttcgacggcc agtgtcaggt gatcaagccg gccgaagaca tgaaggcggt
     5941 cacaggcgtc agctgcacac agaccggcag cgaccacggc atcgagctcg acgccgtgat
     6001 catcggtggc gagatcgtcg tactgaagct cgagacgcac gtcggcgttg cgccggatcc
     6061 gatggctcgc acggaagtca cgcgaatcaa agcgcccgtc ggttctgccg tctcgccgga
     6121 aggctgacta ccctcgcggg tactagccgg ctgcacgacg gcgagcgatc gtcgtgcgat
     6181 gcggtgggta ttcgtgttgg tagcgttggg ggcgtgcggc gacggcggta catcgcaggt
     6241 ccccgcggat tccccttcgg tcgattcgtc ggtgggcccg tgcgatccga ttgcgcgaac
     6301 aggttgtgga gcgggctcgc agtgcacgtg gttctacgaa ggctcggggc accttggttg
     6361 tgcctcgcag ggaagcgtcg ggatcaacga gcgttgtctg cgcaacgaga acacgcacac
     6421 ggacaactgc gctccgggcc ttgcctgcgg tgcgtacggg ttatgtcgac ggatctgcga
     6481 tccctcgtcg aacgcgggat gtacagacct cgcggggaca acgtgtatcg ccgaggagcc
     6541 cgcattcggt agtgcgtcgc cgattgcagg actctgcgaa accatttgcg acccgctcac
     6601 cgacaacgac ttcacgggaa cgggcactac cggacactgc ggcgcctcgc atgggtgtta
     6661 tgggtttccg agttacggaa ccttgccact cacgttctgg acgtgcatgg acgcgggcgg
     6721 aactaacctt gttcatcgcg ccgagctcgg gcccgtcctc tatgtcaact cgtgcgcccc
     6781 cggttacctg ccgctcttgc gcgagagctc cacggtctcg acgacaattt gcgtcgcggt
     6841 ctgcaagcca ctcaactgct acgcgggcaa ctgcggcacc aatgcggaga atcgcctagg
     6901 cgctgcgccg catcgctgca tgaatcccga cgctgccggc acgttcgaca cgggcccaaa
     6961 tggcgagcag tgcgagttcg tgtggcgtcg cgagatcgat ccgatggcgg ggacgtacct
     7021 gccctcgacg acgagcgaca ctctcggctt ctgtttcgac cactcgaagt atctgtacgc
     7081 cgcaaacggc ggaggcccgg acaccccgct gccggcgtgc tcggacctcg ccgatggctt
     7141 tggctcagga agcgatccaa gcagcccgct cacgtactgg ggcgcggccg atctgggctg
     7201 tgtcgactcg acgcatgcgg gagtccacgc cgccaccggc aaaccgcttc ggcgctctcc
     7261 gctcgacgat ctgcggccct tcttcggctc acagcatctg tcatcagctc gcagcgagat
     7321 ccggacgacc ggtaggaatt ggcaacggcc atgaccacgc cacgttgagc gcggcgtcga
     7381 cgtgagcgac gcgcctacac cggaacttgc ccccacccat gtccactcct tagcgtggag
     7441 gatggacggt gttgcgctct tcgatactgg tgttggttct cgcgagcggc tgtgtggccg
     7501 gcggcgatga gcctactgcc acgatcgaga tcaagaccga cacgacgacc gctgtgacga
     7561 tcgatcaccc ggtcgcggag cttcgcgatg cagtgagcga ggcatcggtt cccgacgtct
     7621 gctcgctcgc cgccgagctg ccgacgagcg atgtctgttc gctcatctgt gatccgccgg
     7681 cgatggcgca gtacctgatc gacgagggca tgcacggcgg cacctgctat cagcttcggt
     7741 gcgtgctgcc cggccgtgag aacgcggtca tggtcggcgt ctgtttgctc tgagccgcat
     7801 tcttcgcgcg acctttctcg accgtgctgc cactaggcgg tcatgaagcg attgggaatg
     7861 gttgcggcgg cgctcgtggg ctgtacgaac ggaggcagcc ccgcgtcgcc ggacgcggca
     7921 ggcgtccagc cggatgcgcc agcgctcgcg cccgcggtgc ccggtgcatg tcacgagagc
     7981 atcacgctgt acggcttgga cggcacgaac ggacgcaaga ccgtcggacc gctcgcgctc
     8041 gacacgcaag gcgtgacgct gtgcctgacg ctcgatgcga cgcagaacat cgtcgtgggg
     8101 cacttcggtg cggattcagg ccgcgagcag gcggcagcgt cgacgtttgc gatgtcgctg
     8161 ttcgatgcga atggcacgct gctgcgcgac ggctgggacg tcacgttcgg cagctcgcca
     8221 ccgacgacgt ttgcgaatct cgagtacggc gtgacgaagg gaacgatcct cgaagcgaag
     8281 ctcgtgattc gcacgcgagc agggcaggcg agcacgagcg tcgggctgga tctgatcgag
     8341 ccgtacgagt agctcagtac ttcgtcggat cgttcgggaa cgcgtcgcac tcgcagacgt
     8401 actgcctgcc gagaccgcag ctgcgcatct cgaggtcgcc ggtgttcgac atgaggacgc
     8461 agtcttcggc accgctggct ttgggctcgt cggttcccca cggacctccc gtcgacggcg
     8521 ggtagttgtt ggtctccgcg gtcacccatt ggtacgcgcc ctcgatcgcc cgatccgaga
     8581 gaccgaccca tttttcaccg ccgagtgccg catcgagcgc agagagctcg gcgtcgttcg
     8641 ctgcgacgat caggtgcgtg aagcgcgtgc tgcccgtgat ttggtcggcg aggcaggctg
     8701 cctgcgcaac atcccaggcc gtgccaccgt tgacgaaccg atagcgcgat ggcgacgtgc
     8761 cgagcaccat cgaataggac tgcgggcaga gcgcgttact cggagtgtcg gcgggtgcat
     8821 cggctatacg cgcatccgac gatgcatcgc tgacgctcgc atcttcgtcg acggttccgt
     8881 catcgagcgt cggcggctcg aggccgaaca ggcggccgca gcctgtcagc gacaccgcga
     8941 ggacgaggac gcgccacacg gagcgatcgt atcaccgcgt cgcgtagtcg gtgagaccga
     9001 tgcgaaaccg ctcgaggatg aagaaattga gcgggatacg cagcgggccg accgcggcgt
     9061 acgccttgcc gaggaacagg tcgggattgg ccggatcgac ctgcacgagg tagtcgcgaa
     9121 gctgcgacat cgggttgagc ccgttgtcag ccttgccgta gtcgagcagc accgcgtgca
     9181 gatacgcgtt gtcgcgcgac gtcgcgtcga cgtgatcggt ctcgtagaag ccaaaccgct
     9241 tcgcgccgac gttccaccga ccgtcgagca cgttgttctt caccggcgag ttgtagccca
     9301 tcaccttgcc gtcctcgcgg cggtgaaagc ctttgacgaa cttcttgatg ccggcgagct
     9361 gcgcaagcgg gatggcgttg agcggcaggt gattgatgcc gcggaactcc cagcccacga
     9421 gcgaatcgag atcgggcgtc gcgccgcgca cgaacacttt ctcgaggcca gacatcggcg
     9481 tttgctcgag gcgaagcgct gcttccgacg gaaatgcgct tgccttgtac gtgggcagcg
     9541 tgcgcgacga gcgagacgac gagaatgcag ttgcagtggt catgacagga gcctccggct
     9601 gttgtcgatc acgtagcccg ccgcgcgagt ggcgagcgtc atgatggttt ccatcgggtt
     9661 gacgcgaatc ggcgtcggga acagtgacgc atcggagatg agcaagcggt cgacgtcgtg
     9721 catgaagccg tactcatcgg tgaccgcctt gaggcgatcc gtgcccatcg agcacgtgcc
     9781 catcatgtgg accgtgacga cttcccacgc actcgctggg atgggcttgc tgaagaactt
     9841 ccgcgtgtcg tcggccgaga gcatttcgct cgcgtggtgg aacggcagca acactttctt
     9901 cgcgcccgcc gcgaacagca gctccgagag cagcgtcagg cccttctgca tcagcgcgat
     9961 gtcgcgatcg acgagctgat agaacgcctg cggtcggcca ttgatcgtgc gcacgcgacc
    10021 ggtcgtcgag tcctccatca gcaggccagc gacgaacatg ttgttgtact gctgcatgat
    10081 ctcgccgagc tgcttgccct tgtggggcag cgacatcgcg acaatgctcg gcggcacgtt
    10141 gatcgcggcg aacgttccga tgccttgatc gacgaactcg cggacctgga atgcttgatg
    10201 gacacccttc cagctcgtca cgtcctcgtc gaagatcggg acgaccttga cgttcgggtg
    10261 cagcgtgagg ttgccgccga gcatgcccga gcgcgactcg agtcccgagc gacgcagcag
    10321 cgccggcgta tggatcgcgc cgcacgatgc gacgacgagc ttcgcccgca cgacgacgcg
    10381 ccagctctcg ccaccgcctt cgcgttggac gcggccgatc acgcccgtcg cgcgcttgcc
    10441 gtgaaacgtc aggcgatcga ctttgacgtc ggcgtagacg cgagcgccga agtgcagcgc
    10501 acgcgggatg tagctgacga gcgcgctctg cttcgcgccg gtcgggcagc caaacgggca
    10561 gcggttgcta ccggcgcaat gcacctgatt gcgtaggttg ctgaccgtct tccagcccat
    10621 cgcatcggcg ccctttttca ggagcaagtt gtcgcgaccg atcgcggctt cgtcgttctg
    10681 cgcgacgttg atgcgcttct cgacgcgctc gtaatacggc tcgagctcct tgggcgtgat
    10741 cgagagtccg gcctcgttgc gccagcgcgc gaggatgtcc tcgggcgtgc gccacgacat
    10801 gccgccgttg atcaccgtcg agccgccgac ggcgcggccc tcttggaaca ggatcggcgg
    10861 attgccgagc gccatcgtcg cgccaccgtc gcggtacagc tggcgaacca tcgccgaggt
    10921 gt
//
LOCUS       DATLBZ010000211         3642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2447104, whole genome shotgun sequence.
ACCESSION   DATLBZ010000211 DATLBZ010000000
VERSION     DATLBZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447104"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VMZ53_11085"
     CDS             <1..376
                     /locus_tag="VMZ53_11085"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS29051.1"
                     /translation="LMIDHIGIAVSDLERSVAFYTKALAPLGYELIMKFEGQAAGFGV
                     KGKPDFWIGKGDVKAQAHVAFRAGGRAEVRAFYEAALANGAKDNGAPGPRPHYHEHYY
                     GGFVHDPDGHNIEAVCHEPFLG"
     gene            complement(639..1667)
                     /gene="selD"
                     /locus_tag="VMZ53_11090"
                     /pseudo
     CDS             complement(639..1667)
                     /gene="selD"
                     /locus_tag="VMZ53_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            complement(1757..>3642)
                     /locus_tag="VMZ53_11095"
     CDS             complement(1757..>3642)
                     /locus_tag="VMZ53_11095"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29052.1"
                     /translation="AGRYRIVSKLGEGGMGAVYRGEQMSLKRAVAVKLLRPELSTNQM
                     ILPRFNAEAQAVAKLSHPNTVNIYDFGQDTDGSLFIAMEFIEGRSLREAIQREGPFQP
                     ARALQIASQVAASLSDAHSHNIVHRDLKPDNVMLQDRGKHKDVVRVLDFGIAKLRDDS
                     RATQAAMTQAGDMLGTPQYMAPEQIKGEPIDGRTDVYALGCMIYEMITARLPFEAPTV
                     MAMLSKHLMEMPPPPSQRRPELHLSPAIDQLVMAALAKDSRQRPPTMDAYAEMITAML
                     ATLPGDPNRSAPMSIQQPVVSGTAMTPVPAYPHAAAPAFTPAPPSPFGPPQMPPPGRP
                     MPPGPPGPPGPPGPQHGGYVPAPISPTMPVSAPHGAPPMHPHSLPPAHIAKPKGSKAP
                     LFAIIGVVVAAGAGVGVWQATKSDNKDATEEKGSADVGPDDPDKDDPDKDDPDDTHDT
                     PEPTKPDPWADNAEVDGVKALGGSRKPLPDTAITKKSVGTAKTPLPAGAYITPPPGFV
                     ETKLSNDSKSYINEDRNLLLLIAPLEAGTNDPKKLAAAWTKATGAKLIKQDKAPSHGA
                     SRDVVMFTTEYGGVAVMQIVVLYITDSYRIGVMLQTPLALFEDPAFETEIDQIYENNV
                     HLP"
BASE COUNT          586 a         1182 c         1218 g          656 t
ORIGIN      
        1 gctcatgatc gatcacatcg gaattgcggt cagcgatctg gaacgcagcg ttgcgttcta
       61 cacgaaggcg ctcgcgccgc tcggctacga gctcatcatg aaattcgaag gccaagcggc
      121 aggcttcggc gtgaagggca agcccgactt ctggatcggc aaaggcgacg tgaaggcaca
      181 agcgcacgta gcattccgcg caggtggtcg cgcggaagtg cgcgcgttct acgaagcggc
      241 gctggcgaac ggagccaaag acaacggcgc accgggcccg cgcccgcact atcacgagca
      301 ctactacggc ggcttcgtgc acgaccccga tggccacaac atcgaggctg tctgccacga
      361 accgttcctc ggctagcttg cgacgcaagc ggcttgaatt gcgacgcgac ggcctgattg
      421 cgacgcgacg gctggatcgc gacgcgaccc cgacgagata ccggtgatca ccaggtgtct
      481 tttcggatca cgtcatgacg ttgtactgac gagtaacttc ccggacttca tcgtcatgac
      541 gccgtcatga cgggtttccg gaggacacct actgagcacc gggatctgct catcgggatc
      601 tgatcaccga gagctgatca cgaggatctg ctccagcact acccgatgag cgtgatgcgc
      661 ggacctcgac ctggcgcggc gaggaatccg atgtgcgccg cggggaggcc ggcgtcgcgc
      721 agctccgcga cgcattcctt cacgcgttcc ggcggcaagc agagcagcag gccgccactc
      781 gtctgcgcgt cgcacgcgag cgaggcgagc agctcgcgat cgctgtcgtc accttcggcg
      841 gtgagcggcg gcccatcgag ccatcgcgta tctgcagcga catgcgcgcg gtttgctttg
      901 ctgcccgccg ggcagagctt cgcgcgcagg tgctccatcg cgtcgggaag cagcggcagc
      961 gccgcgaggt cgaccgtcgc cgcgacgtcc gaaccccgga cgatattgcg caggtgaccg
     1021 aggaggccaa agcccgtgac gtcggtcgcc gcggtaacgc catgcttgtg gccgatgcgc
     1081 gcggcagatg cgttcagcgt cgtcatcgac ttgatggcgg ccgcagcagt ggattcgctg
     1141 gcgaggcctt tcttgatcgc ttgcccgatc acgccggtgc cgagcgcctt cgtgaggatc
     1201 agcgtctggc caggtcgcgc ggtgcgattg ctccacagct cgccgggacc gacatcgccc
     1261 gtgacggaga gcccgaactt cacctcggga tcacgcacgg tgtgaccacc gacgacggcg
     1321 acacctgctt cgcggcagat gcgcgcgcca cctgcgagga tcgcgtcgag aatctcgagc
     1381 ggaagctggt cgtcgggaaa gaacacgagg ttgagcgcga accggggctc gccgcccatt
     1441 gcccatacgt ccgagagtga gttggcagcc gcgatcatgc cgaacgcttc tgcatcatcg
     1501 acgagcggcg cgatcacgtc agcggtgagc acgaggtcgc gaccacctgc aacgcgcacg
     1561 atcgcagcgt catcggagta ggcgtgatcg acgacgatgt cagggcgcac ctctcccgat
     1621 ccccgttttt cgaggtgatc gagaacatgt gccagctcgc cgagccggag cttggcggct
     1681 caaccaccgc cgcgcgcgta ttgcgtgagg cgaatggctg cctttgcttc aggcgtcatg
     1741 cccgtcagcg tagcacctac ggcaggtgga cgttgttctc gtagatttgg tcgatctcgg
     1801 tctcgaacgc cggatcttcg aacagcgcga gcggtgtctg cagcatcacg ccgatgcgat
     1861 agctgtccgt gatgtagaga acgacgatct gcatgaccgc gacgccgccg tactcggtcg
     1921 tgaacatcac gacgtcgcgc gatgcaccgt ggctcggcgc cttgtcctgc ttgatgagct
     1981 tcgcgccggt cgccttcgtc cacgcagcgg caagcttctt cggatcgttc gtgcccgctt
     2041 cgagcggcgc gatgagcagc aacagatttc gatcttcgtt gatgtacgac ttcgaatcgt
     2101 tgctgagctt ggtctcgacg aagcccggcg gcggcgtgat gtacgcgcct gcgggcagcg
     2161 gcgtcttcgc agtgccgacg ctcttcttcg tgatcgcggt gtcgggtagc ggcttgcgcg
     2221 agccgccgag cgctttgacg ccatcgacct cggcgttgtc ggcccacgga tccggcttcg
     2281 tcggctcggg cgtgtcgtgg gtatcgtcgg gatcgtcttt gtcaggatcg tctttgtcgg
     2341 gatcatccgg accgacgtcg gccgagcctt tctcttcggt cgcgtccttg ttgtcactct
     2401 tcgtcgcttg ccacacgccg acgccggcac ccgcggcgac gaccacaccg atgatcgcga
     2461 acagcggcgc cttgctgcct ttcggcttcg cgatatgcgc cggcggcagc gaatgcggat
     2521 gcatcggcgg ggcaccatgc ggtgcgctca cgggcatcgt cggcgagatc ggtgcgggca
     2581 cgtatccgcc gtgctgcggt cccggtggcc ccggcggccc cggcggcccc ggcggcatcg
     2641 gtcgacccgg cggcggcatc tgcggcgggc caaacggcga cggtggtgcg ggcgtaaatg
     2701 cgggcgcagc agcgtgcggg tacgccggca ccggcgtcat cgcggtaccc gagacgaccg
     2761 gctgctggat cgacatcggc gcgctgcgat tcggatcgcc gggcagcgtc gcgagcatcg
     2821 ccgtgatcat ctcggcgtac gcatccatcg tcggcgggcg ctgccgcgag tctttcgcga
     2881 gcgcggccat cacgagctga tcgatcgcag gcgacagatg cagctccggt cgacgctgcg
     2941 acggcggtgg cggcatctcc atcagatgct tgctcagcat cgccatcacc gtcggtgctt
     3001 cgaacggcag ccgcgcggtg atcatctcgt agatcatgca gccgagcgcg tagacgtcgg
     3061 tgcggccgtc gatcggctcg cccttgatct gctcgggcgc catgtactgc ggcgtgccga
     3121 gcatgtcgcc ggcttgcgtc atcgccgctt gcgtcgcgcg cgaatcgtcg cgcagcttcg
     3181 cgatgccgaa gtcgagcacg cgcacgacgt ccttgtgctt gccgcgatcc tgcagcatca
     3241 cgttgtcggg cttgagatcg cggtggacga tgttgtgcga gtgcgcgtcg gacagcgagg
     3301 ccgcgacctg cgatgcgatc tgcagtgcgc gcgccggctg aaacggtccc tcgcgctgga
     3361 tcgcttcacg cagcgagcgt ccctcgatga actccatcgc gatgaacagc gagccgtcag
     3421 tgtcttgacc gaagtcgtag atgttgaccg tgttcggatg cgacagcttc gcaaccgcct
     3481 gcgcctcggc gttgaagcgc gggaggatca tctgattcgt cgacagctcg ggccgcagca
     3541 gcttcaccgc gaccgcgcgc ttgagcgaca tctgctcgcc gcgatagacc gcgcccatgc
     3601 cgccttcgcc gagcttgctg acgatgcgat agcgacccgc ga
//
LOCUS       DATLBZ010000212        43418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9796372, whole genome shotgun sequence.
ACCESSION   DATLBZ010000212 DATLBZ010000000
VERSION     DATLBZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 43418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43418
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9796372"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VMZ53_11100"
     CDS             <1..991
                     /locus_tag="VMZ53_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29053.1"
                     /translation="EVCPMGKLTGTKTLSGPFSTDATNTMCDSTQLWRSTNANTCFVV
                     GNTITVTDLQVTGTRPLALVANTINVTGHLDAASRVTDGKTGPNSTANNTCGNPGTPS
                     TGNGAGGGVGASFAPQGMKAGSGGNGFGGGGASAINPFLTVPPGTLRARCRGQTGATG
                     NGGGGLGGYGGGAVYLLGAQTISIGAAGIVDACGAGGGTAGKEAGGGGGGSGGMIILW
                     AGTFSLTAGAIVVANGGSGSTGGDSNQGGAGTRGNDPDYANPQTPAPGPTTPAGKGGN
                     GFAQGTSATAATNGGEGTATGDGGGGGGGAGGYIQSNMALTNAKVSPAANVVQ"
     gene            1016..1258
                     /locus_tag="VMZ53_11105"
     CDS             1016..1258
                     /locus_tag="VMZ53_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29054.1"
                     /translation="MADDALPKPEPKEIDEKMALQLKHLMEDNVLKGQPYGDEKCGNC
                     LYYLNTDDAIAYCWHPKLRILVGTQWWCQWWEKIED"
     gene            1255..2091
                     /locus_tag="VMZ53_11110"
     CDS             1255..2091
                     /locus_tag="VMZ53_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29055.1"
                     /translation="MRQEEKEVDGPEIEHEEERKRESELVEEKEDEIHDLDGKPYEMD
                     GQRDKDRSGMVVGAMRKILEGEQIVINDLKLPQRELKALEALQTAKRGRDGQLDQFVY
                     AEDRRALLEQALAVLQPDIVTLEKEAGQPFEELMKDVAGLRDKLNVLEDSEEELFQRR
                     VENEKGETDSDDKPKPSDDDQTLSGPERKVEKKTSDLGGAERKIDKPKSTLDGPEVKE
                     EKKAKSTLDGPEVKEEKKPTTLTGGEAKEPKKPDTSLGEPKEIDDAQKKLPWWKAGSS
                     RG"
     gene            2172..4244
                     /locus_tag="VMZ53_11115"
     CDS             2172..4244
                     /locus_tag="VMZ53_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS29056.1"
                     /translation="MAELPAWLLGVVREVRLHAEAALLRRLNAGAGFADARVASQIYL
                     RDEEAMARLAGGTLRPQGTHAKAARELEAKLASAATDARGTRFATLTSRLGIDPVGGG
                     LVAAALAYEVDLDMRELVHALAPRRKPALYLETCVDVLGLPATEILLAVGPGSALRRG
                     RVISVQGDGLAADIEITGSAMTWLLGDDSLQPPLAGLCELIPPNVSLGVVLPKPVTTA
                     IDEVVPRAREAHVAVVAKGPRGSGRKAAAHRLAKALERPLVVIPIPEVLTAEKRGRTM
                     LLGAALAAARLRDAVPYLADADALYDESGNMLAEHADAIAGLPGVIVVGSTGRGGHLE
                     LRRPQLIVAMPRTELADREQAWTHALQARKAPTTHAAELAARYVIGPGAIAETVAEAH
                     RFAQAAAVPLELETLEGAVARRLSLRLGTFGSVVSRKARMSELVLPDDVVDTLRDMIA
                     MVRERAQILERWGYQRHLGISRGVAGLYSGEPGTGKTMAASVVASELGLELVRIDLSA
                     VVSKYVGETEKNLGRIFDEAQDAHAMLLFDEADSLFGKRTELKSAQDRFANLEVNYIL
                     QRMETFDGVSVLTTNAESSIDPALQRRLNFRIRFPEPEIEEREKLWKQLLPPATGLHD
                     NVDFHALAERFDMTGGYIKNAVVRAAVIAARAGRAMVAEDLWMGAHHEYVEMGKVMPQ
                     MTPTSSGL"
     gene            4257..5075
                     /locus_tag="VMZ53_11120"
     CDS             4257..5075
                     /locus_tag="VMZ53_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS29057.1"
                     /translation="MSLRGKTLFITGASRGIGLAIGVRAARDGANVVIAAKTAEPHPK
                     LPGTIHTAAKEIEDAGGKALPLVCDVRQEENVAAAVAKAVETFGGIDILVNNASAINL
                     TGTEMTDMKRYDLMHSINTRGTFLCSKLCIPHLKKAANPHVLNLSPPLNMEQRWFAPH
                     VAYTMAKFGMSMCVLGMSGEYKSAGIAFNALWPRTTIATAAINNLLGGGNMMKASRKP
                     EIMADAAYVIFNRPAKEFTGNFCIDDEVLASAGKTDLSEYAVDPSQKLMPDFFV"
     gene            complement(5072..5518)
                     /locus_tag="VMZ53_11125"
     CDS             complement(5072..5518)
                     /locus_tag="VMZ53_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29058.1"
                     /translation="MTRFLVLLAVVIASGCAKNESQGESSSGTSSAKPADPNAPLPAF
                     AVKPVEPSEAQDIATLKQRTSPQVSKWLSEQTTSDGFIVTYEIPRIEDVQDETTLRFE
                     RKQTGVDFGVYLRRNIDGHPHKCVTTRIAKREDIARVVEACKTAKP"
     gene            complement(5515..6132)
                     /locus_tag="VMZ53_11130"
     CDS             complement(5515..6132)
                     /locus_tag="VMZ53_11130"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUS29059.1"
                     /translation="MAKLRITYNAPVVLTFTLAAVAVFCLTRLVTPMQSWFQASPELD
                     SIQSYVGLFSHILGHASWEHLLGNFMLILLIGPILEERHGSGPLLVMILLTALITGIA
                     NALLSSAGLQGASGIVFMMILLASMANMKSREIPLTFIAVAIIYLGGEIVHSFRSDNI
                     SQLAHLVGGAAGAAFGFFMAGKRGKAAEPKAVVAAGLDLKGLKAK"
     gene            complement(6196..6777)
                     /locus_tag="VMZ53_11135"
     CDS             complement(6196..6777)
                     /locus_tag="VMZ53_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUS29060.1"
                     /translation="MSVEHAQVVVAYDFSKSAHIALTRAVALACRAPWHVLQIVCVID
                     PHFPFPAVPTDDKVDIHYADRVQEAAGKVVADELAKQDAKRAVHFFVHARIGKAAPEI
                     LGVAKDIGADLIMLGSRNVTGIERFLLGSVAERVVREAGCAVEIVKEKAYSYVPLLEV
                     TETPHQDHQYVRPHRYTYEDDRVERRPDDWPLY"
     gene            6962..7474
                     /gene="trxA"
                     /locus_tag="VMZ53_11140"
     CDS             6962..7474
                     /gene="trxA"
                     /locus_tag="VMZ53_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HUS29061.1"
                     /translation="MATVEITADNFKETIEKGGIVLLDFWAEWCGPCRAFAPVFEKAS
                     DSHTDVVFGKIDTEAQQELAGAAGIQAIPTLMLFRDGVLLFRESGALPPAALEELVSQ
                     AKKLDMAQVKKDIEEHKANHVHGPDCDHDHDHDHGHEGHDHGHDHGHAHGDAGHVHGP
                     DCKHGHDHDH"
     gene            7515..10148
                     /locus_tag="VMZ53_11145"
     CDS             7515..10148
                     /locus_tag="VMZ53_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29062.1"
                     /translation="MRQLVVGCMLCCAGLAHADDDVHDVFGFNKKPKQAPLDCSDGTA
                     FGCSDASDPLSEREQPYALTTWLPATYLLSLPVADSTHDQVASYGLGASRDETGASFA
                     GATGLENRWTVEGAPADGVRTGAADTRIPLAFLEGIRITAGGFTARDRASTGGTIDAE
                     LRRGTKTHEVEARAYLGWEAEARRRPIGPASYFTRRGVQDNGPSLSTSLVATGPLGAL
                     LGGSGWYAVGVAPEVDSTKFTFRSSTLTDMDGDGIPDGLPGVVNTQPVDKLEQTPITY
                     RVPFMLRGGLDRGVHHLDVSLVGTASSDARFLFNSTLQAAGVNGRTIAGDAIATWRGT
                     WKTTRARAQIAWHRTMRHEYARDPAAEDKPQLLSAYIPTTLDEDPFLAGACNDDIGSD
                     RWPTITNCPVPFGWFSSGGAGPMADVTGDRPSITADIAHKSGDHVVRAGVTGEDTRLV
                     TASRFSGGAQVRSLFDGHTSTRRFVDQDAACSTDLALPCPTVDTSVLNWRTRYTAAYL
                     EDTWHADPKVKFDGGMRWELMWVGTSLHFSHELAPRFGVSWDPLGGGRSRIWTSIGRS
                     YALLPAGLGSTILSRDRTVDLAQSPFGQSRSVDQGAVYPVVANVKPLAQDEATIGAQV
                     ALANAVRATTWLQGRWLREGLDTTQSGFDNPGHIAGAPAVRETGVFAFELATAPTAKL
                     VLRAGYMYGRTIGNWTGSYDPRQGITLYSGDDFDATSQGLLGRLPTDMGHRTYIEAQR
                     SAQVGRVKLSVATRLTTASGKPRSIVADSDDGIIYLLPRGSAGRGPIVHQANVRVAAT
                     WHDLDITLDLFNLFDRHTATNVEELYTTGTLHPIDHGTQADLPFLKTESGAEARRNPN
                     AGVATGFQAPFSAFLGVRRAF"
     gene            10264..18018
                     /locus_tag="VMZ53_11150"
     CDS             10264..18018
                     /locus_tag="VMZ53_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29063.1"
                     /translation="MADLSRFSEAVAEVKHSPHAVSAWEEVEALAGDLDKPDDIIALY
                     TETLAGKLEMQVAEMIGERAGRFCDEWFGDDPKVLEKILSRVLQLAPSSESALQRLSV
                     LYTVAERWDDVLGLYDRAIKQSKDKGGKVRWLREAAQLAKDVANQPEKAIGYYQQLLP
                     LVPDDAQVNTGLERLLERHERWADLISLWEGRLESQSKKDREKSRARIAQVWLDNLAD
                     PQSALAALKPLLVEAEDDKEPCALLERVIEAKGATTGVRASALDLLRSHYDATSRPRE
                     VIRVLEKIIQIDPGNTRELREEAGTRLADLGDLAAAMDHYAALLALAPDSAATEEKLR
                     QLAENGGQHERYATGIANAARAGNGTIDPTRKVELLAEAARTRLERLHDTNGAIEILV
                     EASSVSGAAEHEQLTVARRLAALYADTDKPKDRLAVLERQAHLEANDAARAAILSEAA
                     KLAESLGETDRALQLWERRVDSAPNDVSALDARIGILETQERWDDLVTALEARAAKAP
                     TSVQKRADLVRVAQIHHGKRSDRDAAIATWQRVVTDNRDDEEGISALADLLAEAGRWK
                     EMADLLEGASGRATKRTVGRLVRLGDALREHLDEPARALAAYRNAIAIEASSKEARAG
                     LTALLEVQSTRSPAADALAQAMRTTNDVTGVLDLLPARLAEAKDDRTKLALLREAAQL
                     RLEQKHDAAGALADLARAFPLAPRDQLIENQLQSLAKSTGDFTTLGIAYLEAIESLKD
                     DVREAARLRLAYADLATERLGDQQAAAQAYGQVVSVEPGSRRAVQMFAVLGGKQGQWA
                     AAVDAVVKYTENREAFDDELLTILETAAREQSAYDQLASALGDSLAKNRLPGSVAALY
                     YHRLSSLHRDQRSDRKSALTALRKALDLGGERAGWLSELVAMEKEEGNSPQLLEALRR
                     LADADGKDLDALVGAADVASKLGEREQALSTLSAVLGRATAAWRGTAAIRSARSVDAV
                     AKWAIDGLVDLYRTGGRARAAVDTLVEAARLPFDNATRRDMRLRAAQLATTELGDNAA
                     AIDMYRSVLATTPGDLEVIERLAHLLGVEDRVPELLTLRQIQLGLETDPERKLELRLE
                     LAKLVGIVEERGGRLDALRANLEDRPGHEASIDAISQLLSSKAQHKQLADLLENQANR
                     LEQMTEPQRAAKLWARYATVAENDTKEVERAIAGHRRVVALQPTSDSLRALARLNLER
                     GQSAQAVPWLESLLNTVSGGERLAVVHQLAKAHLAAAQPDRAITAIEMNLDDKTPAIE
                     LRTMLADLYRKADLWEPLARHLTRSLPLLQGDALAREFAREAVQLYISKLGSPAAAIP
                     ALETALQLDPTDKDLRAALAIGKRTGGQLPEARQILVELIADFGRRRSPERAALHVEL
                     ARVAQAEGKTDEAMTEMESASKMDASNTTIQRELAEMARAAGQMERAERTYRALLLVV
                     RRTPPGDDEMAVGPSEVLYELHKLAAARSEDDQAKELLESAIESAIQSDAGVRRMRRS
                     LLAHGDGARLIEVLEKRLATNPEAQSQARLLGDMAEALEQVGKHDESLDALIKAVNAK
                     PDRLDLHDKARDLAKRIDKTKKYVESIEQLVDRLRRKDDPPLIANLLMRAGEALENDA
                     SDLKGAANLYRRVEILGEKLAEAFYAQARVAAALGDTAEQARTLDKMFELAGTDTEPT
                     PQQVDALYRLAEIFLGSESRRKQGFELLERAFSAEPRWGQAGKLLKLAAANDADDRVL
                     SMYERVARHGGDQGLLLDFLELRAQQPGSSPQQIREAVDLAVELAHEERAEALLVRAV
                     AAARDTVDGIAGAPWAMLALAERRLAANDLAQARDLTYEIAPLVSDAETVEQVDQLAM
                     RIATRALAHRKIDIAGEVYEFLRERNPSDRAVWQPLLTIYRELGDGDRLGSVVSSTLP
                     NLTTAPERNALRIEHAKFLIENLKRHHDALDVMRDALNDDPDNLEVAQLYEATLRELG
                     DDDGIAEFLWSRFEDAQRRGHRDSTIDVAIRLGDLLEKQGSPDAIRVYRAALIVAPDD
                     REILRRVVAQLSPQDNPREGALLMERLLAVETPERAPELAGKLASMWEAAGDFKGVQR
                     TLELAHKSAPDDSAIHDRLEKWYRDNEQWSELATLMTGDAERMEDGPAVDRLREAASV
                     YSGFLGQPLKAAEVLRKARQRAPQNPELVTDHAAALAAAGELDAAQRAVGEALATVEG
                     VGRTSLLLLRASFRQQLGDDASAVQDLSEAYELDKDRSAESLLAGLERLRVRAERDSD
                     MTTERSATLKLAQLLVQHGDFERGRGLLVAWIERDARDAEPLYMLCDMDESIEHWDGV
                     AAAATRLAYVTEGEAQVAAALRSSEASAKAGRPGEAVPVLELVHQAQPGVEILRNKLR
                     ETYEAAGEYRLLSGILMADADHGDDPALRYANYKRAAELLLYELQDAPAAQVAAQKAL
                     ELQPDDHAALMLNVDVLIQSGQLEDAGRTLEAGIAAQKKRTPELATMQQRMARICGMQ
                     SDKDGQLNWLKKAFDVDRKNAEVAAELAQLATEMQDYELALKPLRAITLMDNPMPVTR
                     PMALLWEAKIEHARGNRAKAELWAKKALREDPQFADAQQFLDELGN"
     gene            complement(18666..19181)
                     /locus_tag="VMZ53_11155"
     CDS             complement(18666..19181)
                     /locus_tag="VMZ53_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29064.1"
                     /translation="MKSLKLLIATLILGSSSLALAQPIVRDHRSDDRFEDRRDDRRDD
                     RYDSRYDDRVEGRQLEQDSRVERFRWRRMRTAPVTLAQNMEVTLRRPAFLSLSGMTRL
                     RFDADEGHAYIHSIILTFSNGAQQTIDVRQRVTSRSMPLTVDIDSRATGVYVYGSTRG
                     RGALDVIGLRR"
     gene            complement(19258..19677)
                     /locus_tag="VMZ53_11160"
     CDS             complement(19258..19677)
                     /locus_tag="VMZ53_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29065.1"
                     /translation="MSLKAIITTLVLGSSSVALAAPSAPVPTLQPALWMRHVVETPKP
                     ILLASSARINGRDIIKVNDLRPFTKLELRAKLGRTNVDKIVITFANGQTQTINCNRAL
                     HANQSFSIDLAGNQRNLKKIVLVGKSGRRASVDVLAI"
     gene            complement(19879..20097)
                     /locus_tag="VMZ53_11165"
     CDS             complement(19879..20097)
                     /locus_tag="VMZ53_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29066.1"
                     /translation="MSLKHFVIVSILGISSFAFAGNDNKPVKVQNDNTRMWTLQAQEN
                     HAAKSNDSKTVTDPGVLVDAQRDPHDKK"
     gene            complement(20128..20718)
                     /locus_tag="VMZ53_11170"
     CDS             complement(20128..20718)
                     /locus_tag="VMZ53_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29067.1"
                     /translation="MRFAPTLIGIVFVVAGSRAAHAERVLSLDFGYLQNQIAVTDQTS
                     IEGSFARFGIKMSGRYLHAGAEAEEGWLTGQTWEAYGYVARADGSTPEPTQSSPLTGN
                     TLALKTFAGAHTTIGALTLGADLALGFRDTWVSSDRGMDVAGRKNERLLEARTRIDYW
                     LSPQFTLGFVASTDLNERRDTSVGAILAIHFPKLGR"
     gene            20746..21525
                     /locus_tag="VMZ53_11175"
     CDS             20746..21525
                     /locus_tag="VMZ53_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HUS29068.1"
                     /translation="MSDAVRYHAEATVGIITLNRPDNRNSMTPELLDAFSAASTAAKA
                     DAGIRCVVVTGTGNCFSSGADFKSNLQREVGASPEERSYAMYVPFLSLLDIDVPIVAA
                     LNGHTVGGGFGLALVCDVRIGAREAKYGANFVKLGLAPGMAISYLLPRIVGLPKANEL
                     LLTGRLVDGDEAERLGILNKCVPAADVMTEAMTIAKTIAGNAPLAVKATKAAIRRGLA
                     LTVREAARQEAVAQAATVDSEDCREGIAALLEKRTPNFTGR"
     gene            complement(21522..21926)
                     /locus_tag="VMZ53_11180"
     CDS             complement(21522..21926)
                     /locus_tag="VMZ53_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29069.1"
                     /translation="MRYVILFAAACGARSPAAPPPSPPSEMGRPISIEWKAEQADGDR
                     VNVLLVVDGKPEQIGLLDAATETEAGTPSTCALRAAHPLRSEFQCGAVNFYAVELHSG
                     ELVVSINDGSHTIEVKRFSVEGDGLAVRPYAL"
     gene            complement(21914..22927)
                     /locus_tag="VMZ53_11185"
     CDS             complement(21914..22927)
                     /locus_tag="VMZ53_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29070.1"
                     /translation="MRVLVVCSLLACTAPAPKPAGPAAPSEPTLVKRADGGAIRESVD
                     VIATLSADAQHTTWLVPGAAQLSLGGTSVPASEGAAMIEVDLLEEQGNDVRVGVRLAN
                     ARFAVWTSRARLLAVLSRDVRVEGDFGIGGNEVVLYAGARVRRIAHEGSRTKIRYVGA
                     LEVEGWAADDAIADRGAAGRAGGGRVPTGHKTLMVTPGTVIRTEPKWIGKQLAVMNQS
                     YFLDTINQLDDGWYEVAYEDADVRVHGFASTRDPPGRTHRRPPPEPQQAQYAPNATVA
                     ARTCLFADGEPIGFLVGDQQVLVEPGARVGWMTITIESAAWGPLRFDARGPNESALEA
                     CGT"
     gene            23066..24112
                     /locus_tag="VMZ53_11190"
     CDS             23066..24112
                     /locus_tag="VMZ53_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HUS29071.1"
                     /translation="MEIGVITNPNSRKNRNRPERVERLQRIVGQMGEVHQTSSVDSIK
                     PVLREFLRKRARYWVADGGDGALHWMLRMGMEVLQEEEFADESVTLPVTLPTKGGTID
                     FVANNVGIEGDAEGILATLRRTLEHGATIEETEVDSMVIDGIQLIDGKDVKFRTYGFA
                     SAAGGVGQRFYSKYYAEKDPNPRTIVKVVANTLASTPIALSPLSRLPLGQYRTYAKDI
                     FAPTPCKVTLDGMVLPGTEFTGVHIASMSINLGNVLRFFGKADQPGLMNAIVGTPSAW
                     GVIRNIPRMIRGQEMKGANVLDRPCRTMILDATGSELLEPIIDGEYYRNVKRLEFRIG
                     PRVRIPKVVAPKAA"
     gene            complement(24109..24720)
                     /locus_tag="VMZ53_11195"
     CDS             complement(24109..24720)
                     /locus_tag="VMZ53_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29072.1"
                     /translation="MDRLAALEAEVKADADAQKARKDAAIEKVRAQRAQQLADKQDLR
                     DRQAALVTKKKPAAREPIIDDDDEPSDADNLRGALELAGKANRVKNELTRRPKKGQKS
                     KSLVKSGVASLALGPIGWLYAGSMREAVPATVGWILLATLLSKILPTVLLMPVLLVVL
                     PLSGIAGVVYAMQFNKTGKRQRLFNDDEKAEKKLLAGRDPDED"
     gene            24772..25110
                     /locus_tag="VMZ53_11200"
     CDS             24772..25110
                     /locus_tag="VMZ53_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29073.1"
                     /translation="MSLRSLLLAATLLAACSSGSMKVRKGEPQTAREKMLAEEKKNPP
                     DKDEPAPPGSKKWGGWRYQGDRKDCIFVVGKKCFKSENAACQAAHCKAPAKCEAEGGG
                     PATMKCVKKG"
     gene            25336..26211
                     /locus_tag="VMZ53_11205"
     CDS             25336..26211
                     /locus_tag="VMZ53_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29074.1"
                     /translation="MPVRGITLLSLLAFASSAAADTPAPPPAEAIHVAVVPGIAVNMD
                     TARVDALSQDLAEALNTELDVVATGGLEVRRQLPADGLPADCVTNPTCTSDVARRTGA
                     TQLLFVVMVDTGSGGSIQIDTTWIDAASGRSASRPAIDLTSAGEAKGKFASSAKVLLP
                     DAPVRPKQTNGGGGIGVNTSMTEAQPRHFTTPAKITAAIAGVGLGVGIAAGLSTRSRY
                     NKCDGDPVHCSDGEKDSIRRMGFIADAGFVVALGCAITTGVLYATSAKESRLVVAPAP
                     TADGTGATLTALGRF"
     gene            26211..28007
                     /locus_tag="VMZ53_11210"
     CDS             26211..28007
                     /locus_tag="VMZ53_11210"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29075.1"
                     /translation="MSVELEESVYDKLIGQVLANRYLIETRLGEGAMGAVYRAKHVKV
                     GRAFAVKVLHARLLEDKKVLQRFEREAELAGRLRHPNVIGVVDVGEMPDGTRYMVMDF
                     AEGPDLATLLSEAPFPPQRVMTLTRQMLEGLYHAHEAGLIHRDFKPENVIVERDSHKA
                     EVPRIVDFGIAILREGGESSDSPGRLTTNGLVLGTPHYMAPEQAVADPIDHRIDLFAL
                     GIMVYEMLCGKLPFDGSGAEVARANLLLDPPLISERVPYLEVDPLLEAFARRLMAKKR
                     DLRPATAKVARELLDLIDKDREAAAAALGVPIASARAPMTTQPSAPVRDQRNHEGTFP
                     PPLRSEAETPQLAPSLKKVIATPVGARSREHRISTEPTDPVKKSRAPMIGAAIVALGI
                     LGGAAAVTLLGGKKDNKPTTQQVATNTTPDDVKQPDKQAEVKQPDVGSAVEVKAPETG
                     SAAIVKTPEAGSATETKTETKTEIKTTPKSGTATVKKGQTGTKTTTQKTGTQTTDTQA
                     VGSQTKVETVVTPPPEVKTPLDANAIAKQYQSVGAALKSLEQAKGTEAVSDLWSRYRL
                     IRITDAMSTQPKRDEAASLLSKLSTEIAARKK"
     gene            complement(28010..29596)
                     /locus_tag="VMZ53_11215"
     CDS             complement(28010..29596)
                     /locus_tag="VMZ53_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29076.1"
                     /translation="MRHHLAKLTPVLLGGCSLLYNPNNISKPPVDATDGPLEIDAAPM
                     IDGAIDAPPNYTVDPTMLTVIDAWPHDIYEGQGLDNSSPVNLVIYGHHFAPDATVAIN
                     PSAGLTVGTPTISANHDYIVVPITAAVSATDTGSAALTITVSETGATPFDLSGKVSLQ
                     YLKSLTNGTLTLPLDTRYARVALPATVTFAGDLASPVRIHSMSSITCGNGTTNGFAAK
                     GGTATNTTGAAGGPGGCSGGNEGAAGGCGAPLIGGGGSTSGANGGGGGGHGTAGTSGG
                     GNNGGAAHGSPLLLTFTGAAADGSDYNQASGGGGGTADGLILASGGGGGGGGGGSVEM
                     VAGGDIKCGAIDVSGGKGADGAGTLGGTGGGGGGAGGALIVRTGRGTIMNASLLASGG
                     GGGAKQGTGSNGGAGGVGRVRVDTAGALPTVTPGAALHRGLSFASSTMPFYTVPNPMV
                     TLLGTAGDSFDMYVVDDAGVAHEGEPKNQMIGTTGMTTLQVTLLPGYNRLCATIKPGV
                     RDKDNAFSLADTCIEIAYLP"
     gene            29697..30929
                     /locus_tag="VMZ53_11220"
     CDS             29697..30929
                     /locus_tag="VMZ53_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29077.1"
                     /translation="MGGMRILVIAMAMLAGCLPRPQPAQPAQPAQPPVQQTAQAQPPP
                     QQPPPQQPPPQQPLPQQPMQPTDPSQPQPVQNGAGYAPPPQGYYPQQGYPQQQYPQQQ
                     YPQQQYPQPYYPPAPAQPWPAPGTVVGGSEGHRRYHDGEVIADFAIVGTMGSIDFLAR
                     QDVQDGNVVTFAVLAGLAGGGGVGYLLTNKYEVDAGTAHATTLGMSLGFANGALMIEP
                     TGWKRGESVLNFLFFGSAIGAGAGFAYGKNANLTSGQATFVANMTLLGTATAALGAIS
                     GSRDGQYGTWENTTLLVGVDGGTAAGMIIAPSLDWSPHRAKLVMASTVIGAFAGGMLG
                     GLLTPTQQGESRSDNGDIVTAAMTAGLWGGFGLGIMMTKRDSPDPRFAQTASASPTTI
                     APWVGHQGTYGVMAGGSF"
     gene            31048..31794
                     /locus_tag="VMZ53_11225"
     CDS             31048..31794
                     /locus_tag="VMZ53_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS29078.1"
                     /translation="MAIASPGITSEQIFSRVQNGSAALVDAWGCAHTIADGSRVGRDP
                     RVDVAVLHASVSSEHATMKAAGGKWQIVDNTSRNGTEVEGHRITEQELQSGATIRLGD
                     VKFYFWPEALPKMEPPRGQGRTAPTRRDELVFSAKVTTPRGHQLDLRQRVDGGVLRIG
                     ESSIELAAMEFGLLQLLVERRRTVADPELAYVAWHEIADALSFRSVQADSENVRELVH
                     RVRRKLGNVQADDLIESKRGVGYRLAGEPG"
     gene            31794..34799
                     /locus_tag="VMZ53_11230"
     CDS             31794..34799
                     /locus_tag="VMZ53_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS29079.1"
                     /translation="MSERRKRQVRWAKRVRRLGLAWQNALEIARAGRLTAPYGAPFEV
                     QHEERVYKLRRYERTPDAGVEPIAAPLLLVPPLMVASEVYDISPDVSAVAYLVRQGCD
                     VWLVDFGAPEREEGGMNRTLDDHVRAVSEAIERVQKATGQSVHLAGYSQGGMFCYQAA
                     AFRRGSGLASIITMGSPVDLHRQLNIDENVTERLVAGLRALVEWPLERIEGLPGMFTS
                     TGFKLLSARKEAMQLVDFVKNLHDRTALEKREAKRLFLGGEGFVAWPGPAFRKFVDEV
                     IVGNRMASGGFVIDGHTVTLADLTCPILYFVGSRDEMGRPGSVRGIKRAAPRVQKMYE
                     VMVKAGHFGLVVGSTALTVTWPGVVEWMRGGVPPTAIDVRHERPTEPLSVADEDAEDD
                     DHDSPLDLAKELVEKTAHAALERVAELGEDLGAYVDNARWQLPRLHRLRNLQDDSLVS
                     LGKTLADQAATIGDRTFFLWRGRAFTYGEANKRVDAIVRGLLACGIRTGTRVGVMMKS
                     RPSHLSVVAACSRLGAVAVMLSPETTDEVLPRALELGEVEVLIVDPETAARVRAATTI
                     KVMVLGGVGEQGELPAGMQAAKRSIPSGVIDMETIDPATVTVPAWYKPDAGRARDLAM
                     VFVSSGKYEPPRATRITNRRWAFSAIGAAAAATLTTRDTVYCCLPLHHPSGSIVAAHS
                     ALAAGSRLALASRFDPATFWEEVRRYGVSVVFYAGEMCRSLVDADPVLGEKNNPVRLF
                     AGSGMRTDVWRQLVDRFGPVGVLELYASTEANTVIANAAGRKVGSLGRPLPGSPDLAV
                     AAYDFATQDLLRDGRGRLVRARLDEPGMLVAQLTPRADIAHIDPKRLIRDAFEVGDTW
                     FVTGDLAKVDTLGDYTFVDKPEYMIMTKQGPVASTRVEDGIYACDCVALCVAMGRPDP
                     DDPAHQVPIAAIQLGSPLDITDLSKAVAALPEYARPREVRVVEEVPLTDGYRPIKRRA
                     FDQPTGSLYRWNQRAQRYDVVTESLRTTG"
     gene            complement(34796..35689)
                     /locus_tag="VMZ53_11235"
     CDS             complement(34796..35689)
                     /locus_tag="VMZ53_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29080.1"
                     /translation="MAIGAGACGTNSVATGLAIDRIEPASGKNDAPVSVDIGGSFHLP
                     VRVNFDTGETVVSSPAASLGAAPLDSVQWSSEQRIAASVPAGLTPGLYDVTVALGDDT
                     ATLAMGYEVLTSEEPPPPPPAPPFMLTGANWLLPCVTNGMPYQTACTCSGGIVTQAKS
                     VGGMAGERWHVTVRIRGVMEQMTYSGGTPTGSTGWYTGGTYGGGNNYYGFTVSAPAST
                     YFLNNGPAAQTNSWPFDYQVTFDVYAGADVTFYAAGQDGVMWEGVTAGDQPISVAGVT
                     DPAQPYNGQWARMDVIDAVAF"
     gene            35761..36459
                     /locus_tag="VMZ53_11240"
     CDS             35761..36459
                     /locus_tag="VMZ53_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29081.1"
                     /translation="MVKALLLTSVLVACASSDPQPQYAQQPYPQQQQAYPQQQPYQQQ
                     PYQQQPYPQQQPQQPVTAARRITINGMPASARDLQTIEALERQWGIRAQDGDYWYDNT
                     TGATGLWGGPMVSLLPAGLALGGPMPAHASGGGNGALTGVFVNGRELHPYDVTRLQQL
                     VGQVYQGRWWVDAQGNFGQEGGAMLGNLYALQQKRSGGAKDSYWKKDDNGSVFVGGGC
                     VRLSNKNVDYMGGC"
     gene            complement(36456..37109)
                     /locus_tag="VMZ53_11245"
     CDS             complement(36456..37109)
                     /locus_tag="VMZ53_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUS29082.1"
                     /translation="METKPNSPRATIRRKPQRGSYDRAVVHRILDEGLVAHVGFAVDG
                     MPYVIPMVYGRLGDELILHGAVASRMLKQGSTSVPMCATVTLIDGLVLARSWFHHSMN
                     YRSVVVFGTSREITDRDEKLAALTVVVDHATPGRSRESRWPDDKELAATRVIALPIEE
                     ASAKQRIGGPLPDEGDDAALPHWAGEIPLALRATTPVADPSCAGVELPVSLRERWLR"
     gene            37129..38646
                     /locus_tag="VMZ53_11250"
     CDS             37129..38646
                     /locus_tag="VMZ53_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HUS29083.1"
                     /translation="MVGSFLPLLIIATYHYMRAWTFPVSVDAASTTPLFLQIARAISD
                     DVTRGRLHPGDPLPGTRTLADELGVHRATIVAAYEELIAQGWATARSGHATTIAPTSP
                     DVLETSRRPRAPHAPRGMGFDLAPVAGERILAPPAITRASTISLWGGVPDTRLIDLDA
                     VGRALRRATRTHGRSLLRYADDPRGHPALRTALASMLAASRGLAVTADDVLVTNGSQQ
                     ALDLAAHALLRPGDAVAIEALGYRPAWAALRRAGAELLAIPVDAEGLRTAELSALLRR
                     RRIRAVYVTPHHQYPTTVVMSPRRRAALLELARVHRFAIVEDDYDHEFHFEGRPILPI
                     ASTDRSGQVIYVGGLAKSVAPGLRTGYIVAPPAALARLAEERAISDRHGMRIAEAAVA
                     ELLDDGHLQRHVRRARRIYLERRDALATALRKKLGDKLAFDVPAGGLTLWARADGIDV
                     EAWRERAERAGVLVQSGKSFAFDGSRVPCLRLGFAAGTPRELAQAVDILATTFRR"
     gene            complement(38643..39746)
                     /locus_tag="VMZ53_11255"
     CDS             complement(38643..39746)
                     /locus_tag="VMZ53_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29084.1"
                     /translation="MKWVCLLALAACSSREHAAPKQQGSAAVAKDAVPARAQLPWKPL
                     EIEGQPVLDVKPMPNTSDGVFVYMGGQAFTAGGMYSLFWKLDKLDAVPARGRRSLPNG
                     SWLTYVESQGEKFVLSLGTSDAQGEHFRTFETADEPIEVVAQIGELRFRVLVFREGDH
                     WRIARSTDEGEHWSTDVLPMKGANVASAVDHGGIDLALTWNDGDKGKAIHLRAEDAEK
                     RKVSVDALPGKITSTCGGEVLWVTTDKNTLQSLPRGAQATFRSAPMIVACNADYAIVK
                     LADGSAQMCSFECSPTTLPKGEASVQALIGKDLVIVEQVGDTLAITRNNKRTEYDAAG
                     VIQRLQLIDFDGTPVLVAYGRSELDPTRWAVLP"
     gene            complement(39743..40312)
                     /locus_tag="VMZ53_11260"
     CDS             complement(39743..40312)
                     /locus_tag="VMZ53_11260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HUS29085.1"
                     /translation="MPRLYLARHGETEWNAIGRLQGHTDVPLNERGRQQARELAAAVR
                     TLGIGSVITSDLARARETGAIIAGELGLGEPRVVAELRERRIGVFEGLTRDECMVRYP
                     EAWAAWHARTGVPDGAEALAEATARMHAVLGTLAEEPGDPALVVSHGGVMRLWLVDVL
                     GRAVPPLHNGITFAIERDGAAWSAVMLRG"
     gene            complement(40312..41076)
                     /locus_tag="VMZ53_11265"
     CDS             complement(40312..41076)
                     /locus_tag="VMZ53_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29086.1"
                     /translation="MPTPRLAFVFALTACGRLSFDDGTHDAAQGDGRNGDSGPIADGP
                     GVDCHVSHASAIFCDGFENGMTPWGYSVIDQGTVDTTTLRAWGGTHALEVHTPDSAIY
                     KSARWGSFLPSAITTGELFVRQYMWLPSSVASSQVSLIVTGNGATPFPSTFMFLLGPE
                     LVISTDTNSFSTPDVLPRDRWVCFEMQTHIDPTAGFVKITFDGSTAFQTPLTDTDVAG
                     GYTNVDVGVHYATDTQPAVSLWIDDVVVDTNPIGCN"
     gene            complement(41098..41817)
                     /locus_tag="VMZ53_11270"
     CDS             complement(41098..41817)
                     /locus_tag="VMZ53_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29087.1"
                     /translation="MRRFLLAALVAGSGACAQDTSPCSEAAAQLTACSDDQRAAFVSA
                     CEETGGADPASLLADDSSAACKAVPSDGKADQATLAATGLCVASMYGVKWTVTALSPT
                     AQPLSAQTKTMLRPLYGSLVDEARVSIGAALPPAIKIAGHVLAVEPAAMTFGSSIFIL
                     QEVADSKSADRLLLTTVHELAHAKQAKTAGGYYNFAVSYCRDMIAVGFDYSHIHLEEA
                     AYAVQDQARSSLQTCGHVTCP"
     gene            complement(41948..42892)
                     /locus_tag="VMZ53_11275"
     CDS             complement(41948..42892)
                     /locus_tag="VMZ53_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29088.1"
                     /translation="MRRIVVLALLLIACEPPDEGGNAPDAGTIMVDAKPGQGSGSSCH
                     PTTCLAQGAQCGSIADGCGGELDCGFCDNWSDSCSNNTCVCEPYCGTRDCGDDGCGGS
                     CGTCDANESCSSGHCKYAGGGTSPPAAWHCNAAYWDKGDGCDCDCGALDPDCGQAGQA
                     LEGCTGLASPTCDPQGLCTGGGTCNAIPTGPYLTLYLVNGTAPTLSGGTIASGRWVAY
                     DVTHYGGASGYVTGGGDGSAIEISGTSWKRVTRRAGGYPNVDENFTASISGNTITLTR
                     GCPSSSTQTMTFSASATTLTISQYTGGKTVVYKYSKKW"
     gene            complement(42935..>43418)
                     /locus_tag="VMZ53_11280"
     CDS             complement(42935..>43418)
                     /locus_tag="VMZ53_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29089.1"
                     /translation="VGGLAGDDAQQRVSATVGEGGSLAFGDHTFGIDFGAHAWHGINL
                     ASSTLHGVGMAPALSNAVNCKALAQAVSAKCYNGACVGHSAEITSLCENSLKSLVHAL
                     ELEISGIEIAEVHFTSGAARLVDENGDGLANRIEMGTWTPDSALGVGTATFTAITAGR
                     "
BASE COUNT         7610 a        14500 c        14382 g         6926 t
ORIGIN      
        1 cgaagtctgt ccgatgggaa agctgacagg cactaagacg ctgagcggtc cgttcagcac
       61 agacgcgacg aacacgatgt gcgacagcac gcagctgtgg cgcagcacga acgcgaacac
      121 ctgcttcgtg gtcggcaaca cgatcaccgt cacggacctg caggtcacgg gcacacggcc
      181 actcgcgctc gtcgcgaaca cgattaacgt cacggggcac ctcgatgctg cgagccgcgt
      241 gaccgacggt aagaccggtc cgaattcgac cgcgaacaac acgtgcggca atccggggac
      301 gccgtcgacc gggaatggtg ctggtggcgg cgtcggcgcg agctttgcgc cgcaaggtat
      361 gaaagccggt tccggcggca atggattcgg cggcggcggc gcatcggcga tcaatccgtt
      421 cctgacggtg ccgcccggaa cgcttcgagc cagatgtcgt ggccagaccg gagctacagg
      481 caatggcggc ggcggcctgg gcggctacgg cggtggcgcc gtttatctgc tgggtgcgca
      541 aacgatctcg atcggcgctg ccggcatcgt cgacgcgtgc ggtgcaggcg gcggaacggc
      601 aggcaaggaa gcgggtggcg gcggtggcgg ttcgggcggc atgatcattt tgtgggccgg
      661 cacgttctcg ctgacggcag gcgcgatcgt cgtcgcgaac ggcggtagtg gctcgacggg
      721 tggcgatagc aatcagggcg gcgcaggcac gcgcggaaat gaccccgact atgcgaaccc
      781 gcaaacgcca gccccaggcc ctacgacgcc cgccggtaaa ggcggcaacg ggtttgcgca
      841 aggcaccagc gcgaccgcgg caacaaacgg cggcgaaggc accgccactg gcgacggtgg
      901 cggcggtggc ggcggcgcgg gcggctacat ccaatcgaac atggcgctga cgaacgcgaa
      961 ggtctcgccc gccgcgaacg tcgtccagta gcggatcggc tatgctcggc ccgtcatggc
     1021 cgacgacgca ttgccgaagc ccgagcccaa agagatcgac gagaagatgg cgcttcagct
     1081 gaagcacctc atggaggaca acgtcctcaa agggcagccg tacggcgacg agaagtgcgg
     1141 caactgcctc tactacctca acacggatga cgccatcgcg tactgctggc atccgaagct
     1201 tcgcatcctg gtcggcaccc agtggtggtg ccagtggtgg gagaagatcg aagattgagg
     1261 caggaagaga aagaggtcga cggacctgag atcgagcacg aagaagagcg caagcgcgag
     1321 agcgagctcg tcgaggagaa ggaagacgag atccatgatc tcgacggcaa gccgtacgag
     1381 atggatggcc agcgcgacaa agatcgtagc ggcatggtcg tcggcgcgat gcgcaagatc
     1441 ttggagggcg agcagatcgt catcaacgac ttgaagctgc cgcagcgcga gctgaaggcc
     1501 ttggaggcgt tgcagaccgc gaagcgcggg cgcgatggcc agctcgacca gttcgtctat
     1561 gccgaggatc ggcgcgcgtt gctcgagcag gcgttggccg tgttgcagcc agatatcgtc
     1621 acgctcgaga aggaagccgg gcagccattc gaggagctga tgaaagatgt cgccgggctg
     1681 cgcgacaagc tcaacgtcct cgaggattcc gaagaggagc tgtttcagcg tcgcgtcgag
     1741 aacgagaagg gcgagacaga ttccgacgac aagccgaagc cgagcgacga cgatcagacg
     1801 ttgagtggac cggagcgcaa ggtcgagaag aagacgtcgg acctcggcgg cgcggagcgc
     1861 aagatcgaca agcccaagtc gacgctcgac gggcccgagg tgaaagaaga gaagaaggcg
     1921 aagtcgacgc tcgacgggcc cgaggtgaaa gaagagaaga agccgacgac gctcacaggc
     1981 ggcgaggcga aagagccgaa gaagcccgac acgtcgctcg gggagccgaa agagatcgac
     2041 gacgcgcaga agaagctgcc gtggtggaag gccggctcgt cgcgtggcta agacgctcgt
     2101 cgcgtggctg agaagccgcc gtcgtggcgg tcgtcgcgtt gctgacgtga tggcgtggtg
     2161 gaggtggtcg cgtggctgag ttgccggcgt ggttgcttgg cgtcgtgcgc gaagtgaggc
     2221 tgcacgccga ggcggcactg ctgcggcgct tgaatgcggg cgcggggttt gccgatgcgc
     2281 gtgtcgcgtc gcagatctat ttgcgcgacg aggaggcgat ggcgcggctt gcgggcggca
     2341 cgctgcggcc gcagggcacg cacgcgaaag cggcgagaga gctcgaggcg aagctcgcga
     2401 gtgcggcgac ggatgcgcgg gggacgcgct ttgcgacgtt gacgtcgcgg ctcggcatcg
     2461 atcccgttgg tggtgggctt gtcgcggctg cgcttgcgta cgaagtggat ctcgacatgc
     2521 gcgagctcgt gcatgcgttg gcgccgcggc ggaagcccgc gctgtatctg gagacgtgcg
     2581 tcgacgtgtt gggcttgccg gcgacggaga ttttgctggc ggtgggaccg ggctcggcgc
     2641 tgcgtcgagg gcgcgtgatc agcgtgcaag gcgatgggct cgcggcggac atcgagatta
     2701 ccgggtcggc gatgacgtgg ctcctcggcg acgattcgtt gcagccgccg ctcgcggggc
     2761 tgtgcgagct gattccgccg aacgtgtcgc tcggcgtcgt gctgccgaag ccggtgacca
     2821 cggccatcga cgaggtggtg ccgcgcgcga gagaggcgca cgtcgccgtg gttgcgaagg
     2881 ggccgcgcgg ctcggggcgc aaagcggctg cgcatcggct ggcgaaagcg ctcgagcggc
     2941 cgttggtcgt cattccgatt cccgaggtgc tgaccgcgga gaagcgcggc cgcacgatgc
     3001 tgctcggtgc ggcgctagct gcggcgcggc tgcgcgacgc ggtgccgtat ctcgccgacg
     3061 ccgatgcgct gtacgacgag agcggcaaca tgcttgccga acatgccgat gcgatcgcgg
     3121 gattgccggg tgtgatcgtc gtcggctcga cgggacgcgg tgggcatttg gagctgagaa
     3181 ggccgcagct catcgtcgcg atgccgcgga cggagctcgc cgatcgcgag caagcatgga
     3241 cgcacgcgct gcaggcacgc aaggcgccga ctacgcatgc ggccgagctc gctgcgcgct
     3301 acgtgatcgg gcccggcgcg atcgcagaga cggtcgcgga ggcgcatcgc tttgcgcagg
     3361 ctgcggcggt gccgctcgag ctcgagacgc tcgaaggcgc ggttgcgcgg cggctgtcgc
     3421 ttcggcttgg cacgtttggc tccgtcgtct cgcgcaaggc gcgcatgagc gagctcgtgc
     3481 tgcccgacga tgtcgtcgac acgcttcgcg acatgatcgc gatggttcgc gagcgcgcgc
     3541 agatcctcga gcgctggggt tatcagcggc atctcggaat ctcgcgcggc gtcgccgggc
     3601 tgtactcggg cgagccggga actggaaaga cgatggccgc atcggtcgtc gcgtcggagc
     3661 tcggcctcga gctcgtgcga atcgatctgt cggcggtcgt ctcgaagtac gttggcgaga
     3721 ccgagaagaa cctcggcagg atcttcgacg aggcgcagga cgcacacgcg atgctgctct
     3781 ttgacgaagc cgactcgctg ttcggcaagc gcacggagct gaagagcgca caggatcggt
     3841 tcgcgaacct cgaggtgaac tacatcctcc agcgcatgga gacgttcgac ggcgtcagtg
     3901 tgctgacgac gaacgccgag tcgtcgatcg atcccgcact ccaaaggcgc ctcaatttca
     3961 ggatccggtt tccggagccc gagatcgagg agcgcgagaa gctgtggaag cagctacttc
     4021 cgccggcgac gggccttcac gacaacgtcg acttccacgc gctcgccgag cgcttcgaca
     4081 tgacgggtgg ctacatcaag aatgcggtcg tccgagcggc cgtcatcgct gcgcgcgcgg
     4141 gacgcgcgat ggtcgccgag gatttgtgga tgggcgctca tcacgagtac gtcgagatgg
     4201 gcaaggtgat gccgcagatg actccgacaa gcagcggtct gtagtagctt ccgcgcatgt
     4261 ctctgcgcgg aaagactctg ttcatcaccg gcgcgagccg cggtattggt ctggcgatcg
     4321 gcgtgcgtgc ggcgcgcgac ggagcaaacg tggtgatcgc ggcgaagacc gccgagccgc
     4381 atccgaagct gcccggcacg atccacaccg cggcgaaaga gatcgaagac gcgggcggca
     4441 aggcgctgcc cctcgtctgc gatgtgcggc aagaggagaa cgtcgccgcg gcggtcgcga
     4501 aggccgtcga gactttcggt ggcatcgaca tcctcgtgaa caacgcgagc gcgatcaatc
     4561 tgaccggcac cgagatgacg gacatgaagc gctacgatct gatgcattcg atcaacacgc
     4621 gcggcacgtt cctctgcagc aagctgtgca tcccgcacct caagaaggct gcgaacccgc
     4681 acgtcctcaa cctctcgccg ccactcaaca tggagcagcg gtggttcgcg ccgcacgtcg
     4741 cgtacacgat ggcgaagttc ggcatgagca tgtgcgtgct cggcatgtct ggcgagtaca
     4801 agtccgccgg catcgcgttc aacgcgctct ggccgcgtac gacgatcgcg accgccgcga
     4861 tcaacaacct gctcggcggc ggcaacatga tgaaggcgtc gcgcaagccg gagatcatgg
     4921 cggacgccgc gtacgtgatc ttcaatcgcc ccgcgaaaga attcaccggc aacttctgca
     4981 tcgacgacga agtgctcgcg tccgccggca agaccgacct cagcgagtac gccgtcgatc
     5041 cgtcgcagaa gttgatgccg gactttttcg tctagggctt ggcggtctta caggcctcga
     5101 cgacgcgcgc gatgtcctcg cgctttgcga tccgcgtcgt cacgcacttg tgcgggtggc
     5161 cgtcgatgtt gcgacggaga tagacgccga agtcgacgcc cgtctgcttg cgctcgaacc
     5221 gcagcgtcgt ctcgtcttgg acgtcttcga tgcgcggaat ctcgtacgtc acgatgaagc
     5281 cgtcgctcgt cgtctgctcg gagagccact tgctgacttg cggtgatgtg cgctgcttga
     5341 gcgtcgcgat gtcctgcgct tcgctcggct cgacaggctt gaccgcaaac gctggcagcg
     5401 gcgcgttggg atcggccggc ttggcggacg acgttcccga cgacgattcg ccctgcgact
     5461 cgttcttggc gcagcccgag gcgataacga ccgcgagcag aacgaggaag cgagtcactt
     5521 cgctttgagg cccttgagat cgaggcccgc ggcgacgact gctttgggct ctgccgcttt
     5581 gccgcgcttg cccgccatga agaagccaaa cgccgcgccc gcagcgccac cgacgaggtg
     5641 agcgagctgc gagatgttgt cgctgcggaa cgagtgaacg atctcgccgc cgagatagat
     5701 gatcgcgacc gcgataaacg tcagcgggat ttcgcggctc ttcatgttcg ccatcgaggc
     5761 gagcaggatc atcatgaaca cgatgccgct ggcgccttgc aggcccgcac tcgagagcag
     5821 cgcgtttgcg atgcccgtga tcagcgcggt gagcaggatc atcacgagca acggaccgga
     5881 gccgtgtcgc tcctcgagga tcggaccgat gagcaagatc agcatgaagt tgccgagcaa
     5941 gtgctcccag ctcgcgtgac cgaggatgtg cgagaagagg ccgacgtaac tctggatgct
     6001 gtcgagctcc gggctcgcct gaaaccagct ctgcatcggc gtcacgaggc gcgtcaggca
     6061 gaacaccgcc actgccgcca acgtgaacgt cagaacgaca ggagcgttgt acgtgattcg
     6121 cagcttcgcc acacctcaat aactagcacg cgagcaggcg caattcccgt cggaagccga
     6181 cgtggcaaat tgcagctagt acaacggcca gtcgtcgggg cgacgctcga cgcggtcgtc
     6241 ttcgtacgtg tagcgatgtg gccgcacgta ctgatgatcc tggtgcggcg tctcggtgac
     6301 ctcgaggagc ggcacgtacg aatacgcctt ctccttcacg atctcgaccg cgcagccggc
     6361 ttcacgaacg acacgttctg cgaccgaacc gagcaggaaa cgttcgatgc cggtgacatt
     6421 gcgggagccg agcatgatca agtcggcgcc gatatctttc gcgacgccga ggatctccgg
     6481 agccgctttg ccgatccgcg cgtggacgaa gaagtgcact gctcgcttcg cgtcctgctt
     6541 cgcgagctcg tccgcgacga ccttgccagc ggcttcttgc acgcgatcgg cgtagtggat
     6601 gtcgaccttg tcgtcggtcg ggaccgcggg aaacgggaag tgaggatcga tgacgcagac
     6661 gatctgcagc acgtgccacg gagctcgaca tgcgagtgca acggcgcggg tcagcgcgat
     6721 atgcgcgctc ttgctgaaat cgtaggcaac gacgacctgg gcatgctcga ccgacatgca
     6781 tcattaatca gcatcgcgcg tgccatcaaa accacacgga tgcaggaggt tgcgacgagc
     6841 gcctttgcct cggtgagtgc agatcgagcg tcgccgcaca gacacctgtt cagctcggcg
     6901 aatgggcagt ttctcgcgga atcccttgcc gttggaagag ccccaagcca acctccgggt
     6961 aatggcgacc gtcgaaatca cggcagataa cttcaaggaa acgatcgaaa agggcggcat
     7021 cgtcctgctc gatttctggg cggaatggtg cggtccgtgc cgtgcgttcg cacctgtgtt
     7081 cgagaaagcg tccgattcgc acaccgacgt cgtgttcgga aagatcgaca ccgaagcgca
     7141 gcaagagctc gccggtgctg ccggcatcca agccattccc acgttgatgc tgttccgcga
     7201 tggcgtgttg ctgttccgcg agagcggtgc gcttccgccg gccgcgctcg aggagctcgt
     7261 gtcgcaggcg aagaagctcg acatggcgca ggtcaagaag gacatcgagg agcacaaggc
     7321 gaaccacgtc catggtcccg actgcgacca cgatcatgat cacgaccacg gtcacgaggg
     7381 tcatgatcac ggccacgacc acggccacgc tcacggcgac gcgggtcacg tccacggtcc
     7441 cgactgcaag cacggccacg atcacgacca ctgaaagcgc gccccactcg cgcggcggtg
     7501 ataccgtcgt gtgagtgcgg cagctcgtcg tgggatgcat gctctgctgc gcgggtctcg
     7561 cgcacgcgga cgacgatgtc cacgacgtat ttggtttcaa caagaagccg aagcaggcgc
     7621 cgctcgactg cagcgatggc accgcgttcg gttgctccga cgcgagcgat ccgctctccg
     7681 agcgcgagca gccctacgcg ctgacgacgt ggctgcctgc gacgtatctg ctgtcactgc
     7741 ccgtcgctga ctcgacacac gatcaagtcg cgagctacgg gctcggcgca tcgcgcgatg
     7801 aaaccggggc gtcattcgca ggcgcgacgg ggctcgagaa tcggtggacc gtcgagggcg
     7861 cacccgccga tggcgttcgc accggtgccg cggatacccg catcccgctc gcatttctcg
     7921 agggcattcg gatcaccgct ggcggcttca ccgcacgcga tcgcgcatcg accggcggaa
     7981 cgatcgacgc cgagctgcga cgcggcacga agacgcatga agtagaagcg cgcgcgtatc
     8041 tcggctggga ggcggaagcg cggcggcgac cgattggtcc ggcgagctac ttcacgcggc
     8101 gcggtgtgca agacaacggt ccatcgctga gcacatcgct cgtcgcgacc gggccactcg
     8161 gcgctctgct tggcggcagc ggctggtacg cggtcggtgt cgcgcccgag gtcgactcga
     8221 cgaagttcac gtttcgctcg tcgacgctca cggacatgga cggcgatggc attcccgacg
     8281 ggcttcccgg cgtcgtcaac acgcagccgg tcgacaagct cgagcagacg ccgatcacct
     8341 atcgcgtgcc gttcatgctg cgaggtggac tcgaccgcgg cgtgcatcat ctcgacgtct
     8401 cgctcgtcgg caccgcgagc agcgacgcgc ggttcttgtt caactcgacg ctgcaggcgg
     8461 ccggcgtcaa cggccggacg atcgccggcg atgcgatcgc gacgtggcgc ggcacctgga
     8521 agacgacgcg cgcgcgagcg cagatcgcgt ggcaccgcac gatgcgccac gagtacgcac
     8581 gcgaccccgc ggccgaggac aaaccgcagc tgctcagcgc gtacatcccg acgacgctcg
     8641 acgaagatcc gttcctcgcc ggcgcgtgca acgacgacat cggcagcgat cgatggccca
     8701 cgatcacgaa ctgtccagtg ccgttcggct ggttctcgag cggcggcgca gggcccatgg
     8761 cggacgtcac cggcgatcgt ccttcgatca ctgcggacat cgcgcacaag agcggcgatc
     8821 acgtcgtgcg cgcgggcgtc accggcgagg acacgcgcct cgtcacggcg agtcggttct
     8881 ccggcggcgc gcaggttcgc tcgctgttcg atggccacac gtcgacgcgg cgcttcgtcg
     8941 atcaagatgc cgcatgctcg acggacctcg ctctgccctg cccgaccgtc gacacatccg
     9001 tgttgaactg gcgcacgcgc tacacggcgg cgtatctcga ggacacgtgg cacgccgatc
     9061 cgaaagtgaa gttcgacggc ggcatgcgct gggagctcat gtgggtcggc acgtcgctgc
     9121 acttttcgca cgagctcgcg ccgcgcttcg gcgtcagctg ggatccgctc ggcggtggtc
     9181 gctcgcgcat ctggacgagc atcggtcgca gctacgcgct gcttcctgcg gggctcggct
     9241 cgacaatcct gtcgcgcgat cgcaccgtcg atctcgcgca gtcgccgttc ggtcagtcgc
     9301 gatcggtcga tcaaggcgcg gtgtatccgg tcgtcgcgaa cgtgaagccg ctcgcgcagg
     9361 acgaagcgac gatcggcgcg caggtcgccc tcgcgaatgc ggtgcgtgca acgacgtggc
     9421 tgcaaggtcg ctggctccgc gaaggtctcg atacgacgca gtcgggcttc gacaatccag
     9481 gccacatcgc gggcgcaccg gcagttcgcg agaccggcgt gtttgcattc gagctcgcga
     9541 cggcgccgac cgcgaagctc gtgctgcgcg ctggctatat gtacggccgc acgatcggca
     9601 attggaccgg cagctacgat ccgaggcagg gcatcacgct gtactcgggc gacgatttcg
     9661 acgcgacctc ccagggcctc ctcggtcgcc tcccgaccga catgggccac cgtacatata
     9721 ttgaagcgca gcgcagcgcc caggttggcc gcgtgaaact ctcagttgcg acgcgcctga
     9781 cgacagcgag cggcaagccg cgaagcatcg ttgccgacag cgatgacggc atcatctatc
     9841 tgctcccgcg gggcagcgcg gggcgtggtc ccatcgtcca tcaagcgaac gtacgggtcg
     9901 ccgcgacttg gcacgacctc gacatcacgt tggatctctt caatctcttc gaccgccaca
     9961 ccgcgacgaa cgtcgaagag ctctacacga cgggcacgct ccacccgata gaccatggca
    10021 cccaggccga cctcccgttc ctcaaaacgg agtctggagc cgaggcgcga cgtaatccaa
    10081 acgccggggt cgcgactgga tttcaagcgc cgttctccgc gtttctcggc gtccgtcgcg
    10141 cattctgaat cagggatttt gcgaggcgtt cattgatctt gcgcgcgaac cggcgtgtta
    10201 cgatttttcg tccgcatcgc tactcggggt cgcgatgacg acgacacgct cgacgaggag
    10261 cccatggctg acctgtctcg attctctgaa gctgtagctg aagtcaaaca ctctccacac
    10321 gcggttagcg cgtgggagga agtcgaggcg ctcgccggtg atctcgacaa gcccgatgac
    10381 atcatcgcgc tgtacaccga gacgctcgcg ggcaagctcg agatgcaagt tgcggagatg
    10441 atcggcgagc gcgccggtcg cttctgcgac gagtggttcg gcgatgaccc aaaggtcctc
    10501 gagaagatcc tctctcgcgt ccttcagctc gctccatctt cggagtcggc gctgcaacgg
    10561 ctctcggtgc tctacaccgt cgccgagcgc tgggacgacg tgctcggtct ctacgaccgc
    10621 gccatcaaac agagcaagga caagggcggc aaggtccgct ggctgcgtga agccgcgcag
    10681 ctcgccaagg acgtcgcgaa tcagcccgag aaagcgatcg ggtattacca gcagctgttg
    10741 ccgctcgtcc ccgacgacgc gcaggtcaac acgggcctcg agcgcttgct cgagcgtcac
    10801 gagcgctggg ccgatttgat ctcgctgtgg gaaggccgcc tcgagagcca atcgaagaaa
    10861 gaccgcgaga agagccgcgc gcgcatcgcg caagtgtggc tcgacaacct cgccgatccg
    10921 cagagcgcgc ttgctgcgtt gaagcccctc ctcgtcgagg cggaagacga caaagagccg
    10981 tgtgcgctgc tcgagcgcgt gatcgaagcg aagggagcga cgaccggcgt tcgtgcatcg
    11041 gcgctcgatc tcttgcgctc gcactacgac gcgacgtcgc gcccgcgcga agtcattcgc
    11101 gtcctcgaga agatcatcca gatcgacccg ggcaacacgc gcgagctccg cgaagaagcc
    11161 ggcacgcgtc tcgcggacct cggcgatctc gcggccgcga tggatcacta cgcagcgctc
    11221 ctcgcgctcg cgcctgattc ggccgcgacc gaagagaagc tgcgccagct cgccgagaac
    11281 ggtggacagc acgagcgcta cgcgacgggc attgcgaatg ctgcgcgcgc cggcaatggc
    11341 accattgatc cgactcgcaa ggtcgagctg cttgccgaag ccgcgcgcac gcggttggag
    11401 cgcctgcacg atacgaacgg cgcgatcgag atcctcgtcg aagcatcgtc cgtgagcggc
    11461 gctgccgagc acgaacagct gaccgtcgcg cgccgcctcg cggctctcta cgcggatacc
    11521 gacaagccga aggatcgcct cgcggtcctc gagcgccagg cccacctcga agcgaacgac
    11581 gccgcacgcg cggcgattct ctcggaggcc gcgaagctcg ccgagtcgct cggtgagacg
    11641 gatcgcgcac tgcagctgtg ggagcgtcgc gtcgacagcg cgccgaacga tgtgtctgcg
    11701 ctcgacgcgc gcatcggcat cctcgagacg caggaacgct gggacgatct cgtgacggcg
    11761 ctcgaagcgc gcgccgccaa ggcgccgacg tcggtgcaaa agcgcgcgga tctcgtgcgc
    11821 gtcgcgcaga tccatcacgg caagcgcagc gatcgcgatg cagccatcgc gacgtggcag
    11881 cgcgttgtca ccgacaaccg cgacgacgaa gaaggcatca gcgcgctcgc tgacctgctc
    11941 gccgaagccg gccgttggaa agagatggcc gacctcctcg aaggagcatc cggccgcgcg
    12001 acgaagcgca ccgtgggacg gctcgtccgt ctcggcgacg cgctgcgcga gcacctcgac
    12061 gagcccgctc gcgcactcgc cgcctatcgc aatgcgatcg cgatcgaagc gtcgagcaaa
    12121 gaggcgcgcg caggcctcac cgcgctgctc gaagtccagt cgacgcgctc gcccgctgcc
    12181 gacgcactcg cgcaagcgat gcgcacgacg aacgatgtca cgggtgtcct cgacctgctc
    12241 cctgcccggc tcgccgaggc gaaggacgat cgcaccaagc tcgcgctgct gcgtgaagcg
    12301 gcgcagctgc gcctcgagca gaaacacgac gccgccggcg ctctcgccga cctcgcgcgc
    12361 gcgttcccgc tcgctcctcg cgatcaattg atcgagaacc agctccagtc gctcgcgaag
    12421 tcgaccggag acttcacgac gctcggcatc gcgtacctcg aagcgatcga gtcgctgaag
    12481 gacgacgtcc gtgaagcagc gcgcctgcgc ctcgcgtacg ccgatctagc gaccgagcgc
    12541 ctgggcgatc agcaagccgc cgcgcaggcg tacggtcaag tcgtctccgt cgagcccggc
    12601 agtcgccgcg ccgtccagat gttcgccgtc ctcggcggca agcagggcca atgggccgct
    12661 gccgtcgatg cggtcgtgaa gtacaccgag aatcgcgaag cgttcgacga cgaattgctc
    12721 acgatcctcg agaccgcggc acgcgagcag tccgcctacg atcagctggc gtcggcgctc
    12781 ggcgattcgc tcgcgaagaa ccggctgccc ggctctgtcg ctgcgctcta ctaccaccgc
    12841 ctgtcgtcgc tgcatcgcga tcaacgtagc gaccgcaagt cggcgctcac cgcgctccgc
    12901 aaggcgctcg acctcggtgg cgaacgcgcg ggctggctct ccgagctcgt cgcgatggag
    12961 aaggaagagg gcaactcgcc gcagctgctc gaggcgcttc gtcgtctcgc ggacgccgac
    13021 ggcaaggacc tcgacgcgct cgtcggtgct gccgatgtcg cgagcaagct cggcgaacgc
    13081 gagcaagcgc tctcgacgct gtcggctgtg ctcggtcgcg cgaccgccgc atggcgtggc
    13141 accgccgcga tccgctcggc tcgctcggtc gacgccgtcg cgaagtgggc gatcgatggc
    13201 ctcgtcgatc tgtatcgcac cggtggtcgc gcacgcgccg ccgtcgacac gctcgtagaa
    13261 gcagcgcgcc tgccgttcga caacgcgact cgccgtgaca tgcgcctgcg cgccgcgcag
    13321 ctcgcgacga cggagctcgg cgacaacgcc gccgcgatcg acatgtatcg cagcgtgctc
    13381 gcgacgacgc ccggcgacct cgaagtcatc gagcgcctcg cgcatctgct cggtgtcgag
    13441 gaccgtgtcc ccgagctgct gacgcttcgc cagatccagc tcggcctcga gacggatcca
    13501 gagcgcaagc ttgagctgcg tctcgagctc gcaaagctcg tcggcatcgt cgaggaacgt
    13561 ggtggccgtc tcgacgcgct ccgcgcgaac ctcgaagacc gccccggtca cgaagcatcg
    13621 atcgatgcga tctcgcagct gctctcgagc aaggcgcagc acaagcagct cgctgacctc
    13681 ctcgagaacc aggccaatcg cctcgagcag atgaccgagc cgcagcgtgc ggcgaagctg
    13741 tgggcgcggt acgcgacggt cgccgagaac gacacgaagg aagtcgagcg cgcgatcgcg
    13801 ggccatcgcc gtgttgttgc gctgcagccg acgagcgact cgcttcgcgc tctcgcgcgc
    13861 ctcaacctcg agcgcggtca gtccgcacag gccgtcccgt ggctcgagag cttgctcaat
    13921 acggtcagcg gtggcgaacg cctcgcggtc gtccatcagc tcgcaaaggc gcacctcgcc
    13981 gctgctcaac cggatcgcgc gatcaccgcg atcgagatga acctcgacga caagacgcct
    14041 gcgatcgagc tccgcaccat gctcgccgat ctctatcgca aggcagatct gtgggagccg
    14101 ctcgctcgtc acctgacgcg cagcctcccg ctcctgcagg gcgatgcgct cgcgcgcgag
    14161 ttcgcgcgtg aagcggtgca gctctacatc tcgaagctcg gctcgcccgc agcggcgatt
    14221 ccggcgctcg agactgcgct gcagctcgat ccgaccgaca aggacctgcg cgctgcgctg
    14281 gcaatcggca agcgcacggg cggtcagctc cccgaggctc gtcagatcct cgtcgagcta
    14341 atcgcggact tcggtcgtcg tcgctcgccc gagcgcgcag cgctgcacgt cgagctcgct
    14401 cgcgtggcgc aggccgaagg caagaccgac gaggccatga ccgagatgga atcggcctcg
    14461 aagatggatg cgtcgaacac gacgatccaa cgcgagctcg ccgagatggc gcgcgctgcg
    14521 ggtcagatgg agcgtgcaga gcgcacctat cgcgccctgc tcctcgtcgt tcggcgcacg
    14581 ccgcccggtg atgacgagat ggcggttgga ccgagcgaag tgctgtacga gctgcacaag
    14641 ctcgcagcgg cgcgcagcga agacgaccaa gcgaaggagc tcctcgaatc tgcaatcgag
    14701 agcgcgatcc agtcggacgc cggcgttcgc cggatgcgtc gctcgctgct cgcgcatggt
    14761 gatggcgcgc gtctgatcga agtgctcgag aagcgtctcg cgacgaatcc cgaagcacag
    14821 agccaggctc gcctcctcgg cgatatggcc gaagccctcg agcaggtcgg caagcacgac
    14881 gaatcgctcg acgcgctcat caaggccgtc aatgcgaagc ccgatcgact cgatctgcac
    14941 gacaaggcgc gcgacctcgc gaagcgcatc gacaaaacga agaagtacgt cgagtcgatc
    15001 gagcagctcg tcgaccggct ccgccgcaag gacgatccgc cgctgatcgc gaacctcctc
    15061 atgcgcgccg gtgaagcgct cgagaacgac gcgagcgatc tcaaaggcgc agcgaacctg
    15121 tatcgccgcg tcgagatcct cggcgagaag ctcgccgaag cgttctacgc gcaagcgcgc
    15181 gtcgccgcgg ctctcggcga caccgccgag caagctcgca cgctcgacaa gatgttcgag
    15241 ctcgcgggta cggacaccga gccgacgccg cagcaagtcg acgcgctgta tcgactcgcc
    15301 gagatcttcc tcggcagcga gtcgcgtcgg aagcaaggct tcgagctgct cgagcgcgca
    15361 ttcagcgctg agccgcgctg gggccaagcg ggcaagctgc taaagctcgc cgctgcgaac
    15421 gacgccgacg atcgcgtgct ctcgatgtac gagcgcgtcg cgcgtcacgg tggcgatcag
    15481 ggtctgctcc tcgacttcct cgagcttcgt gctcagcagc cgggcagctc gccgcagcag
    15541 attcgtgaag cggtcgacct cgcggtcgag ctcgcgcacg aagaacgcgc ggaagcccta
    15601 ctcgttcgcg ccgtcgctgc agctcgcgac accgtcgacg gaatcgccgg cgcgccgtgg
    15661 gccatgctcg cgctcgcaga gcgccggctc gctgcgaacg acctcgcaca ggcacgcgat
    15721 ctgacgtacg agatcgcgcc gctcgtctcg gatgccgaga cggtggagca agtcgaccag
    15781 ctcgcgatgc ggatcgcgac gcgcgctctc gcgcatcgca agatcgacat cgcaggcgaa
    15841 gtctacgagt tcctgcgcga gcgcaatccg tcggaccgcg ccgtgtggca gccgctgctc
    15901 acgatctatc gcgagctcgg tgatggcgat cgcctcggca gcgttgtctc gtcgacgcta
    15961 ccgaacctga cgaccgctcc agagcgcaac gcgctgcgca tcgagcacgc gaagttcctc
    16021 atcgagaacc tcaagcgtca tcacgacgcg ctcgacgtga tgcgcgacgc gctgaacgac
    16081 gatccggaca acctcgaagt tgcccagctc tacgaagcca cgcttcgcga gctcggtgac
    16141 gacgacggta ttgccgagtt cttgtggtcg cggttcgagg atgcgcagcg tcgcggtcat
    16201 cgtgactcga cgatcgacgt ggcgatccgc ctcggcgatc tgctcgagaa gcagggctcg
    16261 ccggacgcga ttcgcgtcta tcgcgccgcg ctgatcgtcg cgccggatga tcgcgagatc
    16321 ctccgccgcg tcgtcgcgca gctctcgccg caggacaacc cgcgtgaagg cgcgctcctc
    16381 atggagcgtc tcctcgccgt cgagacaccg gagcgcgccc ccgagctcgc cggcaagctc
    16441 gcgtcgatgt gggaagccgc tggcgacttc aagggcgtgc aacgcacgct cgagctcgcg
    16501 cacaagtccg cccccgacga cagcgcgatc cacgatcgcc tcgagaagtg gtatcgcgac
    16561 aacgagcagt ggtccgagct cgccacgctc atgaccggcg acgccgagcg catggaggac
    16621 ggccccgcgg tcgatcgcct gcgcgaagcg gcctccgtct actccggctt cctcggccaa
    16681 ccgctcaagg cggccgaagt cctgcgcaag gcgcgccaac gcgcaccgca gaacccggag
    16741 ctcgtcaccg atcacgccgc tgctcttgcc gccgccggcg agctcgatgc agcacagcgc
    16801 gcggtcggcg aagcgctcgc caccgtcgag ggcgtcggtc gcacgtcgct cttgctcctc
    16861 cgcgcatcgt tccgccaaca gctgggcgat gacgcctcgg ctgtccaaga cctcagcgaa
    16921 gcgtacgagc tcgacaaaga tcgcagcgcg gagtctctcc tcgcgggtct cgagcgccta
    16981 cgcgttcgcg ccgagcgcga cagcgacatg acgacggaac gctcggcgac gctcaagctc
    17041 gcgcagctgc tcgtgcagca cggcgacttc gagcgcggcc gtggcctcct cgtcgcgtgg
    17101 atcgagcgtg acgcccgtga cgccgagccg ctctacatgc tctgcgacat ggacgaatcg
    17161 atcgagcact gggacggcgt cgcggcagca gccacgcgcc tcgcctacgt gaccgaaggc
    17221 gaagctcaag tcgcggccgc actccgctcc tccgaggcct ccgccaaagc gggtcgccca
    17281 ggtgaagcgg tcccggtcct cgagctcgtc caccaagcgc agcccggcgt agaaatcctc
    17341 cgcaacaagc tgcgcgagac gtacgaagcg gcaggcgagt accgcctcct ctccggcatc
    17401 ctcatggccg acgccgacca cggcgacgat ccggcgttgc gctacgcgaa ctacaagcgc
    17461 gcagccgagc tcctcctgta cgagctccaa gacgcacccg ccgcccaagt cgctgcgcaa
    17521 aaagccctcg agctccagcc cgacgaccac gccgcgctga tgctcaacgt cgacgtgctg
    17581 atccaatcgg gtcagctcga agacgcaggc cgcacgctcg aagccggcat cgccgcgcag
    17641 aagaagcgca cgcccgagct cgccacaatg cagcagcgca tggctcgcat ctgcggcatg
    17701 caaagcgaca aagacggcca gctcaactgg ctgaagaagg cgttcgacgt cgaccgcaag
    17761 aacgccgaag tcgcggccga gctcgcccaa ctcgcgacgg agatgcagga ctacgagctc
    17821 gcgctcaagc cgctgcgcgc catcacgctg atggacaacc cgatgccagt cactcgccca
    17881 atggcgctcc tctgggaagc gaagatcgag cacgcgcgtg gcaaccgcgc aaaggccgag
    17941 ctgtgggcga agaaggcgct gcgcgaagac ccgcaattcg cggacgcgca gcagttcctc
    18001 gacgagctcg gcaactaagc cgccgctcgc acgcgctctc gacgacggcg gccttcgggt
    18061 cgccgttgtc gtttcacgaa cgcagcggac cggaatcgtc ggcgctgcgt cgctcgcatc
    18121 gcacgcgtcc gacgaagcga tgaccggtga cacgcagccg agtgcggcgg tgtccgctcc
    18181 gggcggccct cgggcgccgc tgcactgggc gtgcgcgttc gctcgaagcg ccgagcgcat
    18241 cgcagcgcat ccgactgcaa acgcgccgag tacgaacccg cgccacgcga cgcgatcgac
    18301 cacgcgaccg accacgcgat cgaccacgcg acggaccaca ccgatcgacc acaccgatcg
    18361 agcgtacgcg atcgaccgcg cgacacgcag ccgagtgcgg tggtgtccga gggctagcaa
    18421 cggagagcga gcgtaggcgc gcgacgagac gcacggccgc gccgtcagat ctccacaggg
    18481 cgtgcgaagc acgccaacga tcgtgcaacg acgagaaccc gcaacgggag cgccggcacg
    18541 cgagcgggtc cggggcggcc ctcgggcgcc gccgcactcg gcgtgcgcgt tctctcgaag
    18601 cgcgtctgtc ggaaacacgc gaagcgtggg cggcgggacg cggaacggcg gcgaaccgcc
    18661 gacgactagc ggcgcaggcc gatgacgtcg agcgcgccac gaccgcgcgt cgagccgtag
    18721 acgtagacgc cggtcgcgcg cgagtcgatg tcgaccgtga gcggcatcga gcggctcgtc
    18781 acgcgctggc gcacgtcgat cgtttgctgc gcgccgttgc tgaacgtcag gatgatgctg
    18841 tggatgtacg cgtggccttc gtcggcgtcg aagcggaggc gcgtcatgcc gctcagcgag
    18901 aggaatgcgg ggcggcgcag cgtgacttcc atgttctgcg cgagcgtgac cggggctgta
    18961 cgcattcggc gccagcggaa gcgctcgacg cgcgagtctt gctcgagctg gcggccttcg
    19021 acgcgatcgt cgtagcggct gtcgtagcga tcgtcgcggc ggtcgtcgcg gcggtcttcg
    19081 aagcggtcgt cggagcggtg gtcgcgaacg atcggttgtg cgagagcgag cgacgagctg
    19141 ccgaggatga gagtggcgat gaggagcttc agcgacttca tgcctggcag acgcgccgct
    19201 cgcgcgcgcg attcagagat tccgtgtgaa ccaatgtgaa cgttgcaggt cgcgcagtta
    19261 gatggcgagg acgtcgaccg acgcgcggcg gccactcttg ccgacgagga cgatcttctt
    19321 caggttgcgc tggttacccg cgagatcgat cgagaacgat tggttcgcat gcagcgcgcg
    19381 gttgcagttg atggtctgcg tctggccgtt ggcgaacgtg atcacgatct tgtcgacatt
    19441 cgtgcggccg agctttgcgc gcagctcgag cttcgtgaac gggcgcaggt cattgacctt
    19501 gatgatgtcg cgaccgttga tgcgcgcgga ggaggcgagc aggatcggct tgggcgtctc
    19561 gacaacgtgg cgcatccaca gcgcgggctg gagggtcggc acaggtgcag acggcgcggc
    19621 gagagcaacg gacgaagaac cgaggacgag cgtggtgatg attgccttga gcgacatgct
    19681 cggactttgt gcagccgcta ggccagcgcg aagtatgcgt cgcgattgag cgcgagctgc
    19741 gagggactgc acctcccgca gatcgacctg cagctttcgc aggtcgccgt cggcagcccg
    19801 cgatccggac gccgaaaagg cgaaacggcg accaagtggc cgccgtcacg cgaggaggaa
    19861 gacgaatgag agagcgcgtt acttcttgtc gtgcggatcg cgctgcgcat cgacgaggac
    19921 gcccgggtcg gtgaccgtct tgctgtcgtt gctcttcgcg gcgtggttct cctgcgcttg
    19981 gagcgtccac atgcgcgtgt tgtcgttctg caccttcacc ggcttgttgt cattgcccgc
    20041 gaatgcgaac gacgagatgc ccagaatcga gacgatcacg aagtgcttca gcgacatgcc
    20101 cttgagacgt ccgagaaact cgccgattca acggcctaac ttcgggaagt gaatcgcgag
    20161 gatcgcgccg accgacgtat cgcgacgctc atttaggtcg gtgcttgcga cgaagccgag
    20221 cgtgaattgc ggcgagagcc agtagtcgat gcgcgtgcgc gcttcgagca agcgctcgtt
    20281 cttgcgcccc gccacgtcca tgcctcgatc gctcgagacc cacgtatcgc ggaagccgag
    20341 cgcgaggtcc gcgccgagcg tgagcgcgcc gatcgtcgtg tgtgctccgg cgaatgtctt
    20401 gagcgcgagc gtgttaccgg tgagcgggct cgactgcgtc ggttccggcg tcgagccatc
    20461 ggcgcgagcg acgtagccgt acgcttccca cgtttgaccg gtcagccagc cttcttcggc
    20521 ttctgcgccg gcgtgcagat agcgccctga catcttgatg ccgaagcgcg cgaacgagcc
    20581 ttcgatcgaa gtttggtcgg tgacggcgat ctggttctgc aggtagccga agtcgagcga
    20641 gagcacccgc tcggcgtgcg ctgcgcggga acctgcgacg acgaagacga taccgatcag
    20701 cgtcggagcg aaccgcatgc tggaatgata gcggtataag gacgcatgtc cgacgctgtt
    20761 cgctatcacg cagaggcaac ggttggcatc atcacgctca accgtcccga caaccgcaac
    20821 agcatgactc ccgagcttct cgacgcattt agcgcggcga gcacggcggc gaaggcagac
    20881 gccggtatcc ggtgtgtggt cgtgacgggt acaggcaact gcttctcgtc gggcgccgac
    20941 ttcaagtcga acctgcagcg cgaagtcggc gcgtcacccg aagagaggtc gtacgcgatg
    21001 tacgtacctt tcctctcgct gctcgacatc gacgtgccca tcgttgcggc gctgaacggc
    21061 cacacggtgg gcggtggctt cgggctcgcg ctcgtctgcg atgttcgcat cggcgcgcga
    21121 gaggcgaagt acggcgcgaa cttcgtgaaa ctcggcttgg cgcccggcat ggcgatcagc
    21181 tatctgctgc cgcgcatcgt cggccttccg aaagcgaacg agctgctgct caccgggcgg
    21241 ctcgtcgacg gcgacgaagc agagcgcctc ggcatcttga acaagtgtgt gccggcggcg
    21301 gacgtcatga ccgaggcgat gacgatcgcg aagacgatcg ctgggaatgc gccgctcgcg
    21361 gtgaaggcga cgaaggcagc gattcgccgt ggtctggcgc tcaccgtccg cgaggccgcg
    21421 cgacaggaag cagtcgcgca agctgcgacc gtcgacagcg aagattgtcg cgagggcatc
    21481 gccgcgctgc tcgaaaagcg cacgccgaat ttcaccggcc gctagagcgc gtacggtctg
    21541 acggcaaggc cgtcaccctc gacggagaat cgcttcactt cgatcgtatg cgagccatcg
    21601 ttgatcgaga cgacgagctc gccggagtgc agctcgaccg cgtagaagtt cacggcgccg
    21661 cattggaact cgctgcgcag cggatgcgcg gcgcgcagcg cacacgtgct cggcgttcca
    21721 gcttctgtct ctgtcgcggc gtcgaggagg ccgatctgtt caggcttgcc gtcgacgacg
    21781 agcagcacgt tcactcgatc gccatcggct tgctccgcct tccactcgat cgagatcggg
    21841 cggcccatct cgctcggcgg cgacggtggt ggggcagcgg gcgagcgagc gccgcatgca
    21901 gcggcgaaga ggattacgta ccgcatgctt ccagtgcgct ttcgtttgga ccgcgcgcat
    21961 cgaaccgcag aggtccccag gctgcgctct cgatcgtgat cgtcatccag ccgacgcgcg
    22021 cacccggctc gacgaggact tgctgatcgc cgacgagaaa accgatcggc tcgccatcgg
    22081 cgaacaagca tgtgcgtgca gcgaccgtcg cgttcggcgc gtactgcgct tgttgaggct
    22141 ctggcggcgg gcggcgatgc gtgcgacctg gtggatcgcg cgtcgacgca aatccatgca
    22201 cgcgaacgtc ggcgtcttcg tacgcgactt cgtaccagcc gtcgtcgagc tggttgatcg
    22261 tgtcgaggaa atagctctga ttcatcaccg cgagctgctt gccgatccac ttgggctccg
    22321 tgcgaatcac cgtgccgggc gtcaccatca gcgtcttgtg tcccgtcgga acgcgcccac
    22381 cgccggcgcg acctgcggca ccgcggtcgg cgatcgcatc gtccgcggcc cagccttcca
    22441 cttcgagcgc accgacgtat cggatcttcg tacgcgagcc ttcatgcgcg atccggcgca
    22501 cgcgcgcgcc tgcatagagc acaacctcgt tgccgccgat tccgaagtcg ccttcaacgc
    22561 gcacgtcgcg agacagcacg gcgagtagtc gcgctcgaga tgtccagact gcaaaccgag
    22621 cgttcgcgag ccgaacaccg acgcgtacgt cgttgccctg ctcctcgagc aagtcgactt
    22681 cgatcattgc ggcgccttcg ctcgcgggca cgctcgtccc accgagcgac agctgcgcgg
    22741 cacccggcac gagccacgtc gtgtgctgcg cgtctgcgga caacgtggcg atcacgtcga
    22801 ctgattcgcg aatcgcaccg ccgtctgcac gtttcacgag ggtcggttcg ctcggcgcag
    22861 cgggaccagc gggtttcgga gcgggagccg tgcacgcgag gaggctgcac acgaccaaga
    22921 cccgcatttg accgacggta aaccgttatg cggtccgcgc gtaacgggct acgcagcaac
    22981 cggtttttgc ctcctcaccc tcatgtgatt ttcgccgctt acacaccgcg tcatctagcc
    23041 caggccttgc acttcactgg ggctgatgga aatcggcgtc atcaccaacc ccaacagccg
    23101 caagaaccgt aaccgcccgg aacgggtgga gcggcttcaa cggatcgttg ggcaaatggg
    23161 cgaggtccac cagacctcct ccgtcgactc catcaagcct gtcctgcgcg agttcctccg
    23221 caagcgcgcc cgctactggg tcgccgacgg cggtgacggc gctctgcact ggatgctccg
    23281 catgggcatg gaagtgctgc aggaggaaga gttcgccgac gagagcgtca cgctcccggt
    23341 cacgctcccc acgaagggcg gcacgatcga cttcgttgcg aacaatgtcg gcatcgaagg
    23401 cgacgcagag ggcatcctcg cgacgctccg ccgcacgctc gagcacggcg cgacgatcga
    23461 agagacggaa gtcgactcga tggtcatcga cggcatccag ctgatcgacg gcaaggacgt
    23521 caagttccgc acgtacggct tcgcgtcggc ggcgggcggc gtcggccagc gcttctactc
    23581 gaagtactac gccgagaaag atccgaaccc gcggacgatc gtgaaggtcg tcgcgaacac
    23641 cctcgcatcg acgccgatcg cgctgtcgcc actctcgcgc ctgccgctcg gtcagtaccg
    23701 cacgtacgcg aaggacatct tcgcgccgac gccgtgcaag gtcacgctcg acggcatggt
    23761 gctccccggc accgagttca ccggcgtgca catcgcatcg atgtcgatca atctcggcaa
    23821 cgtgctccgc ttcttcggca aggcagacca gccgggcctc atgaacgcga tcgtcggcac
    23881 gccgagcgcg tggggcgtca tccgcaacat cccgcgcatg atccgcggcc aagagatgaa
    23941 gggcgccaac gtcctcgatc gcccgtgccg cacgatgatc ctcgatgcga ccggcagcga
    24001 gctgctcgag ccgatcatcg acggcgagta ctaccgcaac gtcaagcgcc tcgagttccg
    24061 catcggcccg cgcgttcgga tcccgaaggt cgtcgcgccc aaagcggctt agtcttcgtc
    24121 gggatcgcgg ccggcgagca gcttcttctc ggccttctcg tcgtcgttga acaagcgctg
    24181 gcgcttgccg gtcttgttga actgcatcgc gtacacgacg ccggcgatgc ccgatagcgg
    24241 cagcacgacg agcaagacgg gcatcagcag caccgtcggc agaatcttcg agagcaacgt
    24301 cgcgagcagg atccagccca ccgttgcagg aaccgcctcg cgcatcgagc cggcgtagag
    24361 ccagccgatt ggcccgagcg cgagcgacgc gacacccgac ttcacgagcg acttcgactt
    24421 ctgtcccttc ttgggcctgc gcgtcagctc gttcttgacg cgattcgctt tgcccgcgag
    24481 ctcgagggca ccgcgcagat tgtccgcgtc actcggctcg tcgtcgtcgt cgatgatggg
    24541 ctcgcgcgcg gccggcttct tcttcgtgac gagcgcggcc tggcgatcgc gcaggtcctg
    24601 cttgtctgcg agttgctgcg cgcgctgcgc gcgaaccttc tcgatcgctg cgtccttgcg
    24661 agccttctgc gcgtcggcat ccgccttgac ctctgcctcg agcgccgcca ggcgatccat
    24721 caaggcttgg tcgtcatccg gcacatcgag actatggcat gatctcccta catgtcgctg
    24781 cgcagtttgc tcctcgccgc cacgcttctc gccgcgtgca gcagcggcag catgaaggtc
    24841 cgcaaaggcg agccgcaaac cgcgcgcgag aagatgctcg ccgaagagaa gaagaacccg
    24901 ccagacaagg acgaaccggc gccacccggc agcaagaagt ggggcggctg gcgctaccaa
    24961 ggcgatcgca aggactgcat tttcgtggtc ggcaagaagt gcttcaagag cgagaacgcg
    25021 gcttgccaag cagcgcactg caaagcgccg gcgaagtgcg aggcggaagg cggcgggccc
    25081 gcaacgatga agtgcgtcaa gaagggctga tgcgtgcggc cgctgctcgc agcgatgcga
    25141 gcgctcgagt caacgctcgc gctgtcgagc acctcgatgg aggacgggca cggcgacgat
    25201 cgacgaccca ccgctcacat gcagcgaata agctagtagg gcggcgtagg ccacacggag
    25261 cgctcacgtg aggagagagg tagggagcca ccgggtggat ccccatgaac cctaccgtta
    25321 gaatcgatgg cacaaatgcc ggtacgggga atcacgcttc tgtcgttgct cgcgttcgcc
    25381 tcctcggccg cagcagacac gccggcgcct ccgccagcag aagcgattca cgtcgcagtc
    25441 gtccccggca tcgccgtcaa catggacacc gcgcgcgtcg atgcgctgag ccaagatctc
    25501 gcggaagcgc tgaacaccga gctcgacgtg gtcgcgaccg gtggcctcga agttcgtcgt
    25561 cagctgcccg cagacggact gcccgcggac tgcgtcacga atccgacgtg caccagcgat
    25621 gtcgctcgcc gcaccggtgc gacacagctc ctcttcgttg tcatggtcga caccgggtcg
    25681 ggcggctcga ttcagatcga cacgacgtgg atcgatgccg cgagcggacg cagcgcgtcg
    25741 cgcccagcga tcgacctgac gtcagcgggc gaggcgaagg gcaagttcgc gtcttccgcc
    25801 aaggtcctgc ttcccgacgc accggttcgc ccgaagcaga ccaatggcgg gggtgggatc
    25861 ggggtcaata catcgatgac cgaagcgcag ccgcgccact tcacgacacc ggcgaagatc
    25921 accgcggcga tcgcgggcgt cggcctcggc gttggcatcg cagcgggact gtcgactcgt
    25981 tcacgctaca acaagtgcga cggagatccc gtgcactgct cggatggcga gaaggattcg
    26041 attcgacgca tgggcttcat cgccgacgct ggattcgtcg tcgcgctcgg gtgtgcgatc
    26101 acgaccggcg tgctgtacgc gacctctgcg aaggagtcgc gcctcgtcgt ggcgcccgca
    26161 ccgactgccg atggcaccgg ggcgacgctg acggcgctcg ggagattctg atgagcgtgg
    26221 agctcgaaga atccgtctac gacaagctga tcggccaggt tctcgcgaac cgctatctga
    26281 tcgaaacgcg tctcggtgaa ggcgcgatgg gcgcggtcta tcgggcgaag cacgtgaaag
    26341 tcgggcgcgc gttcgcggtg aaggtcctgc acgcgcgctt gctcgaggac aagaaggttc
    26401 tgcagcgctt cgaacgcgag gccgagctcg ccggccgcct gcggcatccg aacgtcatcg
    26461 gtgtcgtcga cgtcggcgag atgcccgacg gcacgcgcta catggtgatg gacttcgcgg
    26521 agggtccgga cctcgccacg ctattgtccg aggcaccgtt tcctccgcag cgcgtgatga
    26581 cgctgacgcg gcaaatgctc gagggcctct atcacgcaca tgaagcgggg ctcatccatc
    26641 gcgacttcaa gcccgagaac gtgatcgtcg agcgcgattc gcacaaagcc gaggtgccac
    26701 gcatcgtcga tttcggtatc gcgatcctgc gcgagggcgg cgagtcgtca gattcgccag
    26761 gccgcctcac gacgaacggc ctcgtcctcg gcaccccgca ctacatggcg ccggagcaag
    26821 ctgtcgccga tccgatcgat caccgcatcg acctgttcgc gctcggcatc atggtctacg
    26881 agatgctgtg cgggaagctg ccgttcgacg gctccggcgc agaggtcgcg cgcgcgaact
    26941 tgctgctcga cccgccgctc atctccgagc gcgttccgta tctggaagtc gatccgctgc
    27001 tcgaagcatt cgcgcgacgc ctgatggcga agaagcgcga tctgcgcccg gcgacggcga
    27061 aggtggcgcg cgagctactc gacctgatcg ataaagatcg cgaagccgca gcggcggccc
    27121 tcggcgttcc aatcgcgagc gcgcgggcgc cgatgaccac gcagccatcg gcacctgtcc
    27181 gcgatcagcg caatcacgag ggcacgttcc cgccgccgct tcgcagcgaa gccgaaacgc
    27241 cgcagctcgc gccctcgctg aagaaggtga tcgcgacgcc ggtcggtgcg cgcagccgcg
    27301 agcacaggat ctcgacggag ccgaccgacc cggtgaagaa gagccgtgcg ccgatgatcg
    27361 gcgcagcgat tgtcgcgctc ggaatcctcg gtggtgcggc ggctgtgacc ctgctcggtg
    27421 gcaagaagga caacaagcca accacgcagc aggtcgcgac gaacacaacg cctgacgacg
    27481 tcaagcagcc cgacaagcag gcggaggtga agcagcccga cgttggctct gccgtcgaag
    27541 tgaaggcgcc ggaaactggt tcggcagcga tcgtgaagac gcccgaggca ggctccgcga
    27601 ccgagacgaa gaccgagacg aagacggaga tcaagacgac gccgaagtcc ggcaccgcga
    27661 ccgtgaagaa gggccagacg ggaacgaaga ccacgacgca gaagaccggg acgcagacga
    27721 cagacacgca ggcggtcggc tcgcagacca aggtagagac ggtcgtaacg ccgccccctg
    27781 aggtgaagac accgctcgac gccaacgcga tcgcgaagca gtaccagagc gtcggcgccg
    27841 cgctgaagtc gctcgagcaa gcgaagggca ccgaagcggt ctccgacttg tggtcgcgct
    27901 atcggctcat tcgcatcacc gacgcgatgt cgacgcagcc caagcgcgac gaagccgcga
    27961 gcctgctgtc gaagctatcg accgagatcg cagcgcgtaa gaagtagcgc tacggcaggt
    28021 acgcgatctc gatgcacgtg tccgcgaggc tgaacgcgtt gtccttgtcg cgaacgcccg
    28081 gcttgatcgt cgcgcacagc cggttgtagc ccggcagcag cgtgacctgc agcgtcgtca
    28141 ttccagtggt gccgatcatc tggttcttgg gctcgccttc gtgcgcgacg cccgcatcat
    28201 cgacgacgta catgtcgaac gaatcgccag ccgtgccgag aagagtgacc atcggatttg
    28261 gcaccgtata gaacggcatc gtcgaggacg cgaacgagag cccacgatgc aacgcagctc
    28321 ccggcgtcac cgttggcagg gcacccgcgg tgtcgacgcg aacgcgtccc acgccaccgg
    28381 caccaccgtt cgagcctgtg ccttgcttcg cgccgccgcc tccaccgctc gcgagcagcg
    28441 acgcgttcat gatcgtgccg cgacccgtac gaacgatgag cgcaccgcct gcgccaccgc
    28501 caccgccgcc ggtccctccg agcgtcccgg cgccatctgc gcctttgcca ccggagacgt
    28561 cgatcgcgcc gcacttgata tcgccgcccg cgaccatttc gacagagccg ccaccgccgc
    28621 caccaccgcc accgccgctc gcgaggatga gtccatcggc cgtgccgccg ccgccgccgc
    28681 ttgcttggtt gtagtcggaa ccgtcagccg ccgcgcccgt gaacgtcagc aggagcggcg
    28741 agccatgcgc cgcaccgccg ttgttgccac cgcccgatgt gcctgccgtt ccatggccgc
    28801 caccgccacc gccgttcgcg ccgctcgtcg agccgccgcc tccgatgagt ggagcgccgc
    28861 agccgccggc cgcgccttcg ttgccgcccg agcagccgcc cggccccccg gcagcacctg
    28921 tcgtgttcgt cgccgtgccg cccttcgccg cgaagccgtt cgtcgtgccg ttgccgcacg
    28981 taatcgacga catcgagtga atcctgacgg gactcgctag atcgccggcg aacgtcaccg
    29041 tcgctggcaa cgccacgcgt gcgtaacgcg tgtcgagagg aagcgtgagc gtgccgttcg
    29101 tgagcgattt gagatattgg agcgacacct tgccgctcag atcgaacggc gtcgcgcctg
    29161 tttcactgac ggtgatcgtg agcgctgcgg aacccgtgtc cgtcgcgctg accgcagccg
    29221 tgatgggcac gacgatgtag tcgtggttcg cagagatagt tggagtacca acggtcaggc
    29281 ccgcggacgg attgatcgcg accgtcgcat caggcgcaaa gtggtgcccg tagatcacga
    29341 ggttcaccgg cgagctgtta tcgaggccct ggccttcgta gatgtcgtgc ggccacgcat
    29401 cgatcaccgt gagcatcgtc gggtcgacgg tgtagttcgg cggagcgtcg atcgcgccgt
    29461 cgatcatcgg tgctgcatcg atctcgagcg gaccgtccgt ggcatcgact ggtggcttcg
    29521 agatgttgtt cgggttgtag aggagcgaac aaccgccgag gagtaccggc gtaagctttg
    29581 ccaggtgatg ccgcatcgcg atcgattata tccctggatt ctcgacgggt ttcagctcac
    29641 ctgaccctca ccggtgcctc atggccgtct tgctgagggg tattgataat gaactgatgg
    29701 gtggaatgcg aatcctcgtg atcgcgatgg cgatgcttgc gggctgtctt cctcgtccgc
    29761 agccggcaca accggcgcaa ccggcgcagc ctccggtgca acaaaccgca caagctcagc
    29821 caccgccgca gcagcctccg ccgcagcagc ctccgccgca gcagccactg ccgcagcaac
    29881 cgatgcagcc gacggatcca tcacagccgc agcccgtgca aaacggcgcg ggctacgcac
    29941 caccgccgca ggggtactac ccacaacagg gctatccgca gcagcagtat ccgcagcagc
    30001 aatatccgca gcagcagtat ccgcagccgt attacccacc ggcgcccgca caaccgtggc
    30061 ccgcgcccgg aaccgtcgtc ggtggcagcg agggacatcg ccgctatcac gacggcgaag
    30121 tgatcgcgga cttcgcgatc gtcggcacca tgggttcgat cgattttctg gcgcgccaag
    30181 acgtgcaaga cggcaacgtc gtgacgttcg cggtcctcgc gggcctcgca ggtggcggtg
    30241 gtgttggcta cttgctcacg aacaagtacg aagtcgatgc agggaccgcg cacgcgacga
    30301 cgctcggcat gtcactcggc tttgcaaacg gtgcactgat gatcgagccg accggttgga
    30361 agcgcggtga gtcggtgctg aacttcttgt tcttcggtag cgcgatcgga gccggcgccg
    30421 gcttcgcgta cggcaagaac gcgaatctca cgtcgggcca ggcgacgttc gtcgcgaaca
    30481 tgacgctgct cggcaccgcg accgctgcac ttggcgcgat cagcggcagc cgtgatggcc
    30541 agtacggcac ctgggagaac acgacgctgc tcgtcggcgt cgatggcggc accgcggcgg
    30601 gcatgatcat cgcgccgagc ctcgattggt cgccgcaccg cgcaaagctc gtgatggcat
    30661 cgaccgtcat cggcgcattt gcgggcggca tgctcggcgg cctgctgacg ccgacccaac
    30721 agggcgaatc gcgcagcgac aacggcgata tcgtgaccgc ggcgatgacg gccggcctgt
    30781 ggggcggctt tggcctgggc atcatgatga cgaagcgcga ttcgccggat ccccgctttg
    30841 cacaaaccgc gagcgcatct ccgacgacga ttgcaccgtg ggtcggtcat cagggcactt
    30901 acggagtgat ggccggcggg tcgttttgaa caatcgccgc cagcacttgc accactcccg
    30961 tccgacgggc gaatcttgta ggtgatggtt ccctgcctgc tgtgctcatc gccccggtca
    31021 atcggagtcc tgtgcacgtc tcacggcgtg gcgatcgcgt ctcccggcat cacgagcgag
    31081 cagattttct cgcgcgtgca gaacggatcg gccgcgctgg tcgatgcgtg gggttgtgct
    31141 cacacaatcg ccgatggcag tcgcgtcggc cgtgatccgc gggtagacgt cgcggtcctc
    31201 cacgccagcg tctcgagcga gcacgccacg atgaaggcgg cgggtggcaa gtggcagatt
    31261 gtcgacaaca cgagccgcaa cgggaccgag gtcgagggcc atcgcatcac cgagcaggag
    31321 ctccagagcg gagcgacgat tcggctcggc gacgtgaagt tctatttctg gcccgaggcg
    31381 ctcccgaaaa tggagccgcc gcgtggccaa ggccgcaccg cgccgacgcg ccgcgacgag
    31441 ctcgtgtttt ccgcgaaggt caccacgccg cgcggtcacc agctcgatct gcgtcaacgc
    31501 gttgacggtg gtgtactccg tataggggaa agctcgatcg agctggccgc gatggagttc
    31561 ggtttgctgc agctcctcgt cgagcgccgc cgcaccgtcg cggatcccga gctcgcatac
    31621 gtcgcgtggc acgagatcgc cgatgccttg tcgttccgct cggtgcaagc agatagcgag
    31681 aacgtgcgcg agctcgtgca tcgcgtgcgc cgcaagctcg gcaacgtgca agccgacgat
    31741 ttgatcgaga gcaagcgcgg cgttggctat cggctcgccg gcgaacctgg ctgatgagcg
    31801 agcgccggaa gcggcaggtg cggtgggcaa agcgggtccg ccgattgggg ctcgcgtggc
    31861 agaacgcgct cgagatcgcg cgtgcaggcc gattgaccgc tccgtacggt gcgccgttcg
    31921 aggtgcagca cgaagagcgc gtctacaagc tgcgtcgcta cgagcgcacg cccgatgccg
    31981 gcgtcgagcc gatcgccgcg ccgctgctgc tcgttccgcc gctgatggtc gcgtccgagg
    32041 tctacgacat ctcgcccgat gtctcggcgg tcgcgtacct cgtgcgccaa ggctgcgacg
    32101 tgtggcttgt cgacttcggc gcacccgagc gtgaagaggg cggcatgaac cgcacgctcg
    32161 acgatcacgt ccgcgcggtg tccgaagcga tcgagcgcgt gcaaaaggcg acgggccaga
    32221 gcgtgcacct cgccggctac tcgcaaggcg ggatgttctg ctaccaggcc gccgcgttcc
    32281 gacgtggatc aggcctcgcg tcgatcatca cgatggggag ccccgtcgat ctgcatcgcc
    32341 agctcaacat cgacgagaac gtcacggagc ggctcgtcgc tggtctgcgc gcgctcgtgg
    32401 agtggccgct cgagcgcatc gaaggcttgc ccggcatgtt cacgtcgacg ggattcaagc
    32461 tgctgtccgc gcgcaaagaa gcgatgcagc tcgtcgactt cgtgaagaac ctgcacgacc
    32521 gcacggcgct cgagaagcgc gaggcgaagc ggctcttcct cggcggtgag ggcttcgtcg
    32581 cgtggcccgg tccggcgttt cgcaagttcg tcgacgaagt gatcgtcggc aaccgcatgg
    32641 cgtcgggcgg ctttgtaatc gacggccaca cggtcacgct cgcggatctg acgtgcccga
    32701 tcctgtactt cgtcggcagt cgcgacgaga tggggcgccc cggttcggtg cgtggcatca
    32761 agcgcgcagc gccgcgcgtg cagaagatgt acgaggtgat ggtcaaggcc ggtcacttcg
    32821 ggctcgtcgt cggttctaca gcgctgacgg tcacctggcc cggcgtcgtc gagtggatgc
    32881 gcggtggtgt gccgccgact gcaatcgacg tccggcacga gcgcccgacc gagccgctgt
    32941 ccgtcgcgga cgaagacgcg gaagacgacg atcacgacag cccgctcgat ctcgcaaagg
    33001 agctcgtcga gaagacggcg cacgcggcgc tcgagcgcgt cgcggagctc ggcgaggatc
    33061 tcggcgcgta cgttgacaac gcgcgctggc agctgccgcg gctgcatcgc ctccgcaacc
    33121 tgcaggacga ttcgctcgtc tcgctcggca agacgctcgc ggatcaagcc gcgacgatcg
    33181 gcgatcgcac gttcttcctc tggcgcggtc gcgcgttcac gtacggcgag gcgaacaagc
    33241 gcgtcgacgc gatcgtgcgc ggcttgctcg cgtgcggaat tcgcacgggc acgcgcgtcg
    33301 gcgtgatgat gaagtcgcgg ccgtcgcatc tctcggtcgt cgctgcgtgc agccgcctcg
    33361 gtgcggtcgc cgtcatgctg tcacccgaga cgaccgacga ggtgctgcct cgcgcgctcg
    33421 agctcggcga agtcgaagtg ctgatcgtcg atcccgagac cgctgcccgc gtgcgcgcag
    33481 cgacgacgat caaggtgatg gtcctcggtg gcgtcggcga gcaaggcgag cttcccgcgg
    33541 gcatgcaggc ggcgaagcgc tcgatcccgt ctggcgtcat cgacatggag acgatcgatc
    33601 cggccacggt caccgtaccg gcttggtaca agcccgacgc tggccgcgcg cgcgacctcg
    33661 cgatggtgtt cgtctcgagc ggcaagtacg agccgccccg cgcgacgcgc atcacgaatc
    33721 gccgctgggc gttctcggcg atcggcgctg ctgctgctgc gacgctcacg acgcgcgaca
    33781 ccgtctattg ttgcttgccg ctgcatcatc cgtcgggctc gatcgtcgcc gcgcactccg
    33841 cgctcgctgc gggctcgcgc ctcgcactcg cgtctcggtt cgaccctgcg acgttctggg
    33901 aagaagtccg ccgctacggc gtgagcgtcg tgttctatgc gggtgagatg tgccgctcgc
    33961 tcgtcgacgc cgacccggta ctcggcgaga agaacaatcc ggtgcgtctg ttcgcgggct
    34021 cgggcatgcg caccgatgtg tggcgccagc tcgtcgaccg cttcggtccc gtcggcgtgc
    34081 tcgagctcta cgcatcgacc gaggcaaaca cggtgatcgc gaacgctgca ggtcgcaagg
    34141 tcggctcgct cggtcgaccg ctgcccggca gccccgacct cgcagtggcg gcgtacgact
    34201 ttgcgacgca ggatctgctg cgtgacggcc gcggtcgcct cgttcgcgcg cgtctcgacg
    34261 agcctggcat gctcgtcgcg cagctcacgc cacgcgcaga catcgcgcac atcgatccga
    34321 agcggctcat ccgggatgcg ttcgaagtcg gcgatacgtg gttcgtgacc ggcgatctcg
    34381 cgaaggtcga cacgctcggc gactacacgt tcgtcgacaa gcccgagtac atgatcatga
    34441 cgaagcaagg tcccgtcgca tcgacgcgcg tcgaggacgg catctacgcg tgcgactgcg
    34501 tcgcgctgtg tgtcgcgatg ggtcgccccg atccggacga tccggcgcac caagttccga
    34561 tcgctgcgat ccagctcggc tcgcctctcg acatcacgga tctctcgaag gcagtcgccg
    34621 cgctgcccga gtacgcgcga ccccgcgagg tccgcgtcgt cgaggaagtt ccgctcaccg
    34681 acggctatcg gccgatcaag cgtcgcgcgt tcgaccagcc gacaggctcg ctgtatcgct
    34741 ggaaccaacg cgcgcagcgc tacgacgtcg tgaccgagtc gctccgcacg accggctaga
    34801 acgcgacggc gtcgatcacg tccatgcgcg cccactggcc attgtacggc tgcgccggat
    34861 ccgtgacacc cgcgaccgaa atcggctggt cacccgcggt gacgccttcc cacatgacgc
    34921 cgtcctgccc cgccgcatag aacgtcacgt ccgcgcccgc gtagacgtca aacgtgacct
    34981 gatagtcgaa cggccacgag ttggtctgcg cagcaggtcc attgttcagg aagtacgtgc
    35041 ttgccggcgc gctgaccgtg aagccgtagt agttgttgcc gccgccgtac gtgccgccgg
    35101 tataccagcc cgtcgagccc gtcggtgttc cacccgagta cgtcatctgc tccatcacgc
    35161 cgcgaatacg gaccgtgacg tgccatcgct caccggccat accgccgacg gacttcgctt
    35221 gcgtcacgat gccgccggag cacgtgcacg cggtttgata cggcatcccg ttcgtcacgc
    35281 acggaagcag ccagttcgcg cccgtcaaca tgaatggcgg cgccggcgga ggcggcggcg
    35341 gctcttcgct cgtcaacact tcgtagccca tcgcgagcgt cgcggtgtcg tcgccgagcg
    35401 cgacggttac gtcgtagaga cctggcgtga ggcccgccgg caccgacgca gcgatccgct
    35461 gttcggagct ccactgcacg ctatcgagtg gcgcagctcc gagcgatgct gccggcgatg
    35521 acacgaccgt ttcgccggtg tcgaagttca cgcgcacggg cagatgaaac gaaccgccga
    35581 tatcgacgga gacaggcgcg tcgttcttgc ccgatgctgg ctcgatgcga tcgatcgcga
    35641 ggcccgtcgc gacagaattc gttccacagg cgcccgctcc gatggccacg caaaccgcag
    35701 caactgcgcg ccgaaacatc gtgtccagac tactacatga cgcgttatcc tcgacgggtg
    35761 atggtgaaag cgctcctgct gacgtcggtg ttggtcgcat gtgcatcctc ggatccacag
    35821 ccgcagtacg cacaacagcc gtatccgcag cagcagcagg cgtatccgca acagcagccg
    35881 taccagcagc agccgtacca gcagcagccg tatccgcagc agcagcctca acagccggtg
    35941 accgcggcgc gtcgcatcac gatcaatggc atgccggcgt cggcgcgcga cctccagacg
    36001 atcgaggccc tcgagcggca gtggggcatt cgcgcgcaag acggcgacta ctggtacgac
    36061 aacaccacgg gcgcgaccgg cctctggggc ggtccgatgg tgagcttact gcccgcaggt
    36121 ctcgcgctcg gtggaccgat gcccgcgcac gcatccggtg gcggcaatgg cgcgctgacc
    36181 ggtgtgttcg taaacgggcg cgagcttcac ccgtacgacg tgacgcgcct gcaacagctc
    36241 gtcggccagg tctatcaagg ccgctggtgg gtcgacgcac agggcaactt cgggcaagaa
    36301 ggcggcgcga tgctcggcaa cctgtacgcg ctccagcaga agcgcagcgg cggggccaaa
    36361 gactcgtact ggaagaagga cgacaacggg tcggtcttcg tcggtggcgg ctgcgttcgc
    36421 ctgtcgaaca agaacgtcga ctacatgggc ggctgctagc gaagccaccg ctcgcgcagg
    36481 ctcaccggca gctccacacc cgcgcagctc ggatcagcaa cgggtgtcgt ggcgcggagg
    36541 gcgagcggga tttcgccggc ccagtgcggc agcgcagcgt cgtcgccctc gtcgggaagt
    36601 ggtccaccga tgcgctgctt ggccgacgcc tcttcgatcg gcagcgcgat gacgcgcgtg
    36661 gctgcgagct ccttgtcgtc gggccaccgt gactcgcgtg accgccccgg cgtcgcatga
    36721 tcgaccacga cggtgagcgc cgcgagcttc tcgtcgcgat cggtgatctc gcggctcgtg
    36781 ccgaacacga cgaccgatcg atagttcatc gagtggtgaa accacgagcg agcgagcacg
    36841 agcccgtcga tcaacgtcac cgtcgcgcac atcggcacgc tcgtgctgcc ttgcttcagc
    36901 atccggctcg caaccgcgcc atgcaggatc agctcgtcgc ccagccggcc gtagaccatc
    36961 gggatcacgt acggcatacc gtcgaccgcg aagccgacgt gcgcgacgag accctcgtcg
    37021 aggatgcggt gaacgaccgc gcgatcgtac gagccacgct gcggcttgcg gcgaatcgtg
    37081 gcgcgcgggc tgttcggctt cgtttccata cggacagcat gtcccagcat ggtaggctca
    37141 ttcctaccgc ttttaataat tgcgacctac cactacatgc gcgcctggac gttccccgtt
    37201 tccgtcgacg ccgcgagcac gacgccgctg ttcctgcaga tcgcgcgcgc gatcagcgat
    37261 gacgtcacgc gcggccggct gcatcccggc gatcccctgc ccggcactcg cacgctcgca
    37321 gacgagctcg gcgtccatcg cgccacgatc gtagcggcgt atgaagagct gatcgcgcag
    37381 ggttgggcca ccgcgcgctc tggtcacgcg acgacgatcg ccccaacgtc cccagacgtg
    37441 ctcgagacgt ctcggcggcc tcgcgcaccg cacgcgccgc gcgggatggg cttcgatctc
    37501 gcacccgtcg ccggcgagcg cattctggcg ccgcccgcga tcacgcgcgc gagcacgatc
    37561 tcgctgtggg gcggcgtgcc cgacacgcgg ttgatcgatc tcgatgccgt cggtcgcgcg
    37621 ctccgtcgcg ccacgcgaac gcacggtcga tcgctgcttc gctatgccga tgatccgcgc
    37681 ggtcaccctg ccctacgcac cgcgctcgcc tcgatgctcg ccgcatcgcg gggcctcgct
    37741 gtcactgccg acgacgtgct cgtcacgaat ggcagccagc aggcgctcga cctcgcggcg
    37801 cacgcgctac ttcgcccggg agacgccgtc gcgatcgaag cgctcggcta tcggccggca
    37861 tgggccgcgc ttcgccgcgc cggtgccgag ctgctcgcca ttcccgtcga tgctgaaggt
    37921 ctacgtaccg ccgagctctc cgccctgctc cggcgtcgcc ggattcgagc cgtctatgtc
    37981 acgccgcatc accagtatcc gacgaccgtc gtgatgtccc cgcgccgccg cgccgcgctc
    38041 ctcgagctcg cccgcgtgca tcgcttcgcg atcgtcgagg atgactacga tcacgagttc
    38101 cacttcgagg gtcgccccat cctgccgatc gcgagcacgg accgcagcgg tcaggtcatc
    38161 tacgtcggtg gcctcgcaaa atccgtcgcg ccgggtttgc gcaccggcta catcgtcgcg
    38221 ccgcccgcag cgctcgcacg tctcgcagaa gaacgcgcga tcagcgaccg ccacggcatg
    38281 cgcatcgccg aagcggcagt cgccgagctc ctcgacgacg gccacttgca acgccacgtg
    38341 cgccgcgctc gccggatcta tctcgaacgc cgcgacgcac tcgcaacggc gctgcgcaag
    38401 aagctcggcg acaagctcgc gttcgacgtg cccgccggcg gcctcaccct atgggcgcgc
    38461 gccgatggca tcgatgtcga agcgtggcgc gagcgagccg aacgcgcggg tgtcctcgtg
    38521 cagagcggca agagcttcgc cttcgacggc agccgcgtac cctgccttcg cctcggcttc
    38581 gccgcgggca cgccacgcga gctcgcacag gccgtcgaca tcctcgcgac gacgttccgc
    38641 cgctaaggca ggacagccca gcgcgtcgga tcgagctcgc tgcgcccata tgcgacgagc
    38701 acgggtgtgc catcgaagtc gatcagctgc aggcgctgga tgacgccggc cgcgtcgtac
    38761 tcggtgcgct tgttgttgcg cgtgatcgcg agcgtgtcgc cgacctgctc gacgataacg
    38821 aggtccttgc caatgagggc ctgcacggat gcctcgccct tgggcagcgt cgtcggactg
    38881 cactcgaacg agcacatctg cgccgagccg tcggcgagct tcacgatcgc gtagtctgca
    38941 ttgcacgcga cgatcatcgg cgccgaccgg aacgtagcct gcgcgccgcg cggcaatgac
    39001 tgcagcgtgt tcttgtctgt ggtcacccac aggacttcgc ctccgcacgt gctcgtgatc
    39061 tttcccggca gcgcatcgac ggagaccttg cgcttctccg catcctcagc gcgcagatgg
    39121 atcgccttgc ctttgtcgcc gtcgttccac gtgagcgcga gatcgatgcc gccatgatcg
    39181 acggcgctcg caacgttcgc gcccttcatc ggcagcacgt cggtgctcca gtgctcgcct
    39241 tcgtcggtgg aacgcgcgat ccgccagtga tcaccctcgc ggaacacgag cacacgaaac
    39301 cgcagctcgc cgatttgcgc gacgacttcg atcggctcgt ccgcggtctc gaacgtacgg
    39361 aaatgctcgc cttgcgcatc cgacgtcccg agcgacagca cgaacttctc gccctgcgat
    39421 tcgacgtacg tgagccacga gccgttcgga agcgagcgcc ggccgcgcgc tggaactgcg
    39481 tcgagcttgt cgagcttcca gaacagcgag tacatgccgc ctgccgtgaa cgcttgccca
    39541 cccatgtaaa cgaacacacc gtcgctggtg ttcggcatcg gcttcacgtc gagcactggc
    39601 tggccttcga tctcgagcgg cttccacggc agctgcgcac gcgctggcac ggcgtctttc
    39661 gcgacggccg cactgccctg ctgcttcggc gctgcatgct cacgcgacga gcacgctgcg
    39721 agcgcgagga ggcacaccca cttcacccgc gcagcataac agcgctccac gcggcgccgt
    39781 cgcgctcgat cgcaaacgtg atcccgttgt gcagcggcgg cacggccctg ccgagcacgt
    39841 cgacgagcca cagcctcatc acgccaccgt gcgagaccac gagcgccggg tcgccgggct
    39901 cctccgcgag cgtgccgagc actgcgtgca tgcgagccgt cgcttcggcg agcgcttctg
    39961 cgccgtcggg cactccggtc cgcgcatgcc acgcggccca cgcctcgggg taccggacca
    40021 tgcactcgtc gcgcgtgaga ccttcgaaca cgccgatccg gcgctcgcgc agctccgcga
    40081 ccacgcgtgg ctcaccgagc ccgagctcgc ctgcgatgat cgcgccggtt tctctcgctc
    40141 gcgccagatc gctcgtgatg acgctaccga tcccgagcgt gcgcaccgcc gcggcgagct
    40201 cgcgggcttg ctgccggccg cgttcgttca gcggcacatc ggtgtgaccc tgcagccgcc
    40261 cgatcgcgtt ccactcggtt tcaccgtgtc gcgcgaggta cagccgcggc atcagttgca
    40321 gccgatggga ttcgtgtcga cgacgacatc gtcgatccac agcgagacgg ccggctgcgt
    40381 atcagtcgcg taatgaacgc caacatcgac attcgtgtaa ccacccgcca catcggtatc
    40441 ggtaagcggc gtctggaacg ccgtgctgcc gtcgaaggtg atcttcacga agcctgccgt
    40501 cgggtcgatg tgcgtctgca tctcgaagca cacccagcgg tcgcgcggca aaacgtcggg
    40561 cgtggagaac gaattggtgt cggtgctgat caccagctcc gggcccaaca agaacatgaa
    40621 cgtcgacgga aacggcgtcg ccccgttgcc cgtgacgatc agcgaaacct gcgagctcgc
    40681 gaccgacgat ggcagccaca tgtactggcg gacgaacagc tcgccggtcg tgatcgctga
    40741 cggcaagaac gagccccacc gtgccgactt gtagattgcg ctgtcgggcg tgtgcacctc
    40801 gagcgcgtgc gtgccgcccc atgcccgcag cgtggtcgtg tcgacggtcc cttggtcgat
    40861 caccgagtag ccccacggcg tcatgccgtt ctcgaaaccg tcgcagaaga tggcactcgc
    40921 gtggctcacg tgacagtcga cacccggccc atctgcgatc ggaccgctat ccccgttgcg
    40981 gccgtcgcct tgcgcggcat cgtgtgtccc gtcgtcgaac gacagtcgcc cgcacgcagt
    41041 cagcgcgaag acaaacgcga gccgcggcgt gggcacccgc tcacgatagc agcgtcttca
    41101 cgggcaggtg acatgtccgc aggtttgtaa gctgctgcgc gcttggtctt ggactgcata
    41161 ggctgcctcc tcgagatgga tgtggctgta atcgaaaccc accgcgatca tgtcgcggca
    41221 ataactgact gcgaagttgt agtaaccgcc cgcggtcttc gcctgctttg catgcgcgag
    41281 ctcgtgcacg gtggtgagca gcaagcgatc ggccgatttg gagtcggcga cctcctgcag
    41341 gatgaagatg ctggagccga acgtcatcgc agccggttcg acggcgagca cgtgcccggc
    41401 gatcttgatc gcgggtggta gcgccgcgcc gatcgagacg cgcgcttcat cgacgagcga
    41461 gccgtacagc ggccgcagca tcgtcttcgt ttgcgcagac agtggctgcg cggtcggcga
    41521 gagcgccgtg acggtccact tcacgccgta catcgacgca acgcacaggc ccgtcgccgc
    41581 gagcgttgct tggtccgcct tgccgtcgct cggcaccgct ttgcaggcag cgctcgagtc
    41641 gtcggcgagc agcgacgccg ggtcggcacc gccggtctct tcgcacgccg acacaaacgc
    41701 agcgcgttgg tcgtcactgc aggccgtcag ctgcgccgcg gcctcgctac agggcgaggt
    41761 gtcctgcgcg catgcgccgg aaccggcgac caaggcggcg aggagaaagc ggcgcatgtc
    41821 ccgaccatgt gcgagccgcg tgcccggaaa atcgcggttt tggcagccga tcgcgccatc
    41881 cgccggtccg attcgcgcca gccgtcggac ggggcttttc ccctgatggc tgggggtgtt
    41941 gtcaccccta ccacttcttc gagtacttgt agaccacggt cttgccgccc gtgtactgcg
    42001 agatcgtgag cgtcgtcgcg ctcgccgaga acgtcatcgt ctgcgtcgac gacgacgggc
    42061 agccgcgcgt gagcgtgatc gtgttgcccg agatgctcgc ggtgaaattc tcgtcgacgt
    42121 tcggatagcc accggcgcgg cgcgtgacgc gcttccagct cgtgccggaa atctcgatcg
    42181 ccgagccatc gccaccgccc gtgacgtagc cggaggcacc gccgtaatgc gtgacgtcgt
    42241 acgcgaccca gcggcccgat gcgatcgttc cgcccgagag cgtcggcgca gtcccgttga
    42301 cgagatacag cgtcaggtac gggcccgtcg gaatcgcgtt gcatgtcccg ccgccggtgc
    42361 agagcccctg cggatcgcac gtcggactcg cgagaccggt gcagccttcg agcgcctgac
    42421 ccgcttggcc acaatcggga tcgagcgcgc cgcaatcgca gtcgcagcca tcgcccttgt
    42481 cccaatacgc cgcattgcag tgccacgccg ctggtgggct cgtcccgccg cctgcgtact
    42541 tgcagtgacc gctgctgcag ctctcgtttg catcgcaggt tccgcagctg ccgccgcagc
    42601 catcgtcgcc gcaatcgcgg gtgccgcagt acggctcgca cacgcacgtg ttgttcgagc
    42661 agctgtcgct ccagttgtcg cagaacccgc agtcgagctc gccgccacag ccatccgcga
    42721 tcgagccgca ctgcgcgccc tgcgcgagac acgtcgtcgg gtgacagctc gaaccgctgc
    42781 cctgccccgg cttcgcgtcg accatgatgg tcccggcgtc gggcgcgttt ccgccttcgt
    42841 cgggcggctc gcaggcaatc agcaggaggg caaggacgac gatacggcgc atgcccctac
    42901 aacgcaggcc gcgcgcggag tgggaacaaa aagactagcg gccggccgtg atcgccgtaa
    42961 acgtcgccgt cccgacgcca agtgccgaat ccggcgtcca cgtgcccatc tcgatgcggt
    43021 tcgcgaggcc atcgccattc tcgtcgacga ggcgagccgc gccgctcgtg aagtgaacct
    43081 cggcgatctc gatgcccgag atctcgagct cgagcgcgtg cacgagcgac ttcaggctgt
    43141 tctcacacag cgacgtgatc tccgccgagt gcccgacgca cgcgccgttg tagcacttgg
    43201 ccgaaacggc ctgcgcgagc gccttgcagt tgacggcgtt cgacagcgca ggcgccatgc
    43261 cgacaccgtg caatgtcgag ctcgcgaggt tgatgccgtg ccacgcgtgc gcaccgaaat
    43321 cgatgccgaa tgtgtgatcg ccgaacgcga gcgagccgcc ttctccgacg gtggccgaga
    43381 cgcgctgctg cgcatcatca cccgcgagcc cgccgacg
//
LOCUS       DATLBZ010000213        14263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9150934, whole genome shotgun sequence.
ACCESSION   DATLBZ010000213 DATLBZ010000000
VERSION     DATLBZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14263
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9150934"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..936
                     /locus_tag="VMZ53_11285"
     CDS             403..936
                     /locus_tag="VMZ53_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29090.1"
                     /translation="MRLSAVLVCAFVAQCIVFGVRARTGGVVASYQVPFAVAGTWAGC
                     VLGAAIAKPRERGGTVWLVLALGAIELGLFALFMTVATWVGFSPFVPIVVGVMGGAIL
                     FVVLVSDLQQWQFGVAAAGSIGTVVIASQPSVPDFGDVEFVLLFVAISMVGGMLGRTL
                     RSRIKTRSELPPAQLRK"
     gene            complement(939..1841)
                     /locus_tag="VMZ53_11290"
     CDS             complement(939..1841)
                     /locus_tag="VMZ53_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HUS29091.1"
                     /translation="MSRERFAALLDEPFELSRRDNRWLALAPNLVAFAADDFAGWRRL
                     QQEAWLLDRWRRIGGVPAPRVLREDAVRRVQVRERMHGLTGDELHREDSLSPLYSPAL
                     AANPDVRARLVDAGSAAPLSPFGERLAASYGDLAARIRRSVDTMEAETVAIGPTSRRS
                     LDVDAAIAALDASAAASAAAKAAAHRTRDWLATLTPINAVIHADLHFHNMCAAPSGDI
                     IGVFDLSDSGLDNAESEFLYAQSLGTQFAQIAMAAYGSPLDERAVVHAHLRTALDHVA
                     YHGPTTPRHASVVAWATAVFELLA"
     gene            complement(1851..2582)
                     /locus_tag="VMZ53_11295"
     CDS             complement(1851..2582)
                     /locus_tag="VMZ53_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HUS29092.1"
                     /translation="MQRLRVMTYNIHAARGLDMRVRSGGKPARLARIADVIRSADPHL
                     VALQEVDVGRARSGAMDMASELASMLGMDMRFVPCVERGTERYGICTLSSPSLPILES
                     RELRLPRHHDREQRCALITRHAWDGGEVELLNAHLSIVFRERPHQVAAITQEMLGEAL
                     VIAGDFNMTPWSSAYKVLRRGLTSATRFARTWPAPAPLIPLDHILYRGRLELIRGGAW
                     TEAGARSASDHLPVVAELEHLPMAA"
     gene            complement(2600..3091)
                     /locus_tag="VMZ53_11300"
     CDS             complement(2600..3091)
                     /locus_tag="VMZ53_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29093.1"
                     /translation="MFWVSILSAALLGGTACKKSAEEKAQDKFEKAQDNVKDQREDVR
                     DEQKDVKDKLEDVRDEQKDVNKEKGELVKAQTDLAAARVEYRTRVNERLARIDADIQR
                     LEAKADTKSHEKAAKLRERRNELATKVDHIGDQADAKWDEFKKDTDSKFDALENDIKN
                     DRD"
     gene            3276..5498
                     /locus_tag="VMZ53_11305"
     CDS             3276..5498
                     /locus_tag="VMZ53_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29094.1"
                     /translation="MIGEVIGNYRVVAELGKGGMGVVYRAEHTQLGRPAALKMLLPQF
                     SSDQGIVQRFFNEARAASAIDHPGIVEIYDFGTHRDGRAYIVMALLKGESLEHRLISG
                     PVSPQDGATILAQVAAALAAAHQRGIVHRDLKPDNIFLVPNELVPGGLQVKLLDFGIA
                     KLADEKTAGFKTQTGALMGTPAYMSPEQCMGKADLDHRTDIYSLGCILFHVLCGRPPF
                     TSDQGTGMMIAAHIRDAAPDPRTVNPAIPPDLAAIILRCLEKEPSARYQSATELRNAL
                     VQAGANAPLSKPPVASMGYDATIAPSMSPQLHALGTAPTSAPPLSYAPPASHHPLGTA
                     PTTLGGAAAQMTPVPTVAPSHPTPKKGKGALIAVIGGVAVLAAVGAGVAMTRKTDDKP
                     QVVAAKGSDGSATMPVVVATPDAAVAPLPSGAVDNEKPAEGSDAVPALAPVKCADGKV
                     TNVDTRGRCCWADQVWSESKAKCLGKPTCPPGMKLKGEECTAVVAAGVAPPPHGGHPV
                     VDSTPPPPSAVPIFTVDAKTYQPHESVTITFGSPVSSKSGAQAWVTISEKGAAETSYG
                     NWDYVKDGATTAELKAPRRAGEYEVRVHTDYPTKPYNVRYRIPITVKGKDAIAIDTSG
                     GTGSATTTTPTPAIATETPVDKQKFQLARKNVKLGGDAVLHFQTPLHAKDGEKFWITI
                     VAKDAPPDKYEHYEYVPQDAKTAKIAVPSTAGDYEVRLHANYPTKSTNVVHRARLHVE
                     "
     gene            5510..5908
                     /locus_tag="VMZ53_11310"
     CDS             5510..5908
                     /locus_tag="VMZ53_11310"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUS29095.1"
                     /translation="MQTVTVDVARDPETCWRAFVDVGQLPRWVPGLKRAEIITRAKGL
                     PEEVHFEFARELAYTLVYSYDKEERVVRWQPKLGKNAGVSGFVRFEAIENGWTRITYG
                     LEQGDARGPAERELGDLQRLAEAFRSHVQR"
     gene            5988..8321
                     /locus_tag="VMZ53_11315"
     CDS             5988..8321
                     /locus_tag="VMZ53_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUS29096.1"
                     /translation="MTEQAVPSGNVTTARAGLADHAAAVLDAVADGITVEDAAGNIVY
                     ANTAAAELRDLSRLDLFSTDGATLSPRELPSRYVLAGEPSRELVVKFRVKDTGVERWS
                     LVRSNEMRGPAGERWAVNTFRDITAQKAAEDVARENGRRLELALAAGKMGVFEWDIAA
                     NRVYWSPELEAIHGMAPGTFDGTVEMYRRDMHPVDRERVIAATKRTLERKAPHYLNYR
                     ILRPDGTTRWIEAHGQLELDTAGNPVRLVGISRDITEHHEGVRARIDAILDSITDPFT
                     ILDRDLRVVHVNKTAARLSGRSPEELAFKHLEEISPTAKESPFWPAYQKVLETKAPLH
                     VEEYIAAMDRWFEANIYPIDDGIGIYHRDVTMRKRALELTTRLARHSTLRADVAGALA
                     EERDVPKMLQRCCQLLVEHLDVAFARVWTVDDVGTTLMLQASAGMYTHTNGPHRAVPV
                     GKFKIGRIAASRKPHLTNDVAHDTQIGDPQWAAREQMVSFAGYPLLVDGKLVGVMAMF
                     AKHAMPDDTMSALGGIADVIAQGVVRRHTELELEQKVAELARSNADLEQFAYVASHDL
                     QEPLRMVASYNQLLARRYKGKLGEDADEFIAFTVEGVTRMQRLINDLLAYSRVGTRGS
                     DRVEVDMEKLLRGALQNLERALIEADAQVTHDPLPTITADEGQMLQLVQNLVGNAVKF
                     HGDAKPSIHIGVVNDAKGVTFSVRDNGIGIDPQYFERIFVIFQRLNPREKYPGTGIGL
                     AICKKIVERHGGRIWVESAPGAGSTIFFTIPHQRRAQ"
     gene            8318..8746
                     /locus_tag="VMZ53_11320"
     CDS             8318..8746
                     /locus_tag="VMZ53_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS29097.1"
                     /translation="MTGKPIEILLVEDNPADVRLTQEALKEGKVRNNLQIARDGVEAL
                     ELLLRKDAVRPDLILLDLNLPRKDGREVLAEIKNHPELKTIPVVVLTTSSAEADILKS
                     YNLYANCYITKPVDLDQFVQVIKSIDDFWLTVVRLPTDKR"
     gene            8743..10281
                     /locus_tag="VMZ53_11325"
     CDS             8743..10281
                     /locus_tag="VMZ53_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS29098.1"
                     /translation="MKILLVEDNLADARLFAELLAEVPGRPFQLTSVASFSSALAEVG
                     GHDIVFLDLSLPDAHGIDTVTRMVEAARELPIVVLTGTDDDRVATEAMKLGAQDYLLK
                     AEITPSQIARSARYAIERKHAEHHAKRAHQADEAARRARFLSSISGTITSSLELATTL
                     PDVARLLLPMLGDACAIDLLREDARVDRVARAGEAERLERIAMLGSRVIEERAPLAVN
                     EPGGESVLCLPLMGRGRVVGALTFVMAAPRMFDEELRRLADEAAERIAMGIDNASLYE
                     AAQRAIRGRDELLAVVSHDLRNPLNVVALALQSVAHDPMTVASALPRALRGVDRMQRL
                     IEDLLDVARIDTGTLGLELRPVNVTALVDEVVEQHRQLAAEKKISLKGETMSEHIGAR
                     ADRHRLLQVLANLLSNAFKFTPPGGTIKLGVEVRGERVGISVSDTGPGIPAEHLGHIF
                     DRFWQPERRRDGVGLGLAIVKGIVDAHGGTIEVDSKPGIGTTFRVFLDGAVLRPTGVV
                     AVVA"
     gene            complement(10284..10505)
                     /locus_tag="VMZ53_11330"
     CDS             complement(10284..10505)
                     /locus_tag="VMZ53_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29099.1"
                     /translation="MLRKRGNAWEDEHKLDKEIDDDMRDVATKSGIADVDPVPLSHVA
                     GEGIDPDGDVNAHEEIREQRDRLPDRRYR"
     gene            complement(10553..12811)
                     /locus_tag="VMZ53_11335"
     CDS             complement(10553..12811)
                     /locus_tag="VMZ53_11335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS29100.1"
                     /translation="MSFVPGANEGVAPARCVPCLRVAADTLFVDDGHEVETAMLRLDF
                     AYGDRRVRSDDVRWAGRDRDAESQACRVLESLGAIELTHLEDCAVTPGSVIDYVVAVD
                     GDVHALCAFAAYSIPQLRGLGWRVEIDSEYPWQVVGGDAPLYVAAEPDREKPDWFGLE
                     LGVEIDGQRVNLLPALLEMLDSASGLGALAKSARRCVAVPVGDRRWLPVPPARLKLLA
                     KVLMELYRENGKVRAPAVRAPLVAELCAAVHDGTRPFRWIGDTKFREQAYALAMGPRK
                     TGAMTTPATLRADLREYQRDGVAWLQNLREHGAGGVLADDMGLGKTLQTIAHVLLEKE
                     AGRLDRPAMIVTLTSLVGNWQREIAKFAPSLSVAVYHGPDRKKTTLAGHDIIITTYPL
                     VARDRDELATTPLHLLVLDEAHAIKNGSAQSSEAVRVLDARFRICLTGTPIENHLGEL
                     WSLFDFINPGLLGAKDEFTLQFRQPIEDRGDKVRLEALRDRVRPYILRRTKDAVAPEL
                     PAKTQLVRAVELSGAQRELYESIRVAAHADVRAHIRQRGFAGSQIAILDALLKLRQVC
                     CDPRLVSVDAAREISGSAKLDVLLELVTSQLADGRRILIFSQFARMLGLISESLLARG
                     IGHVTLTGNTPDRQKPIDAFTQGRADVFLISLKAGGAGLNLVGADTVIHYDPWWNPAA
                     QAQATDRAHRIGQTKPVFVHDLIAAGSVEERMLSLQRHKRMLANAILDNGKASSTSLT
                     ERELDDLLAPLA"
     gene            complement(12932..13537)
                     /locus_tag="VMZ53_11340"
     CDS             complement(12932..13537)
                     /locus_tag="VMZ53_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29101.1"
                     /translation="MRTVVVAAVLTALTSVAAAQAPGETMSWPVEEAAPSTTTPEPQT
                     VTVSYRRDILIADGLAVGAIFLGPLGDNEELTGWGMVGYMFAAPVVHIAHGRGVTALQ
                     SLGLRAGLPLLGAYVGYNVGPDDAICVESVSGYDGPSHSSCGGDGSLVGMILGGMAGG
                     VTAMVLDAKYLAKYEKRTAPTWSASIKPARGGLSLGVSGTF"
     gene            13678..>14263
                     /locus_tag="VMZ53_11345"
     CDS             13678..>14263
                     /locus_tag="VMZ53_11345"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS29102.1"
                     /translation="MDNVDVVIIGAGISGIDAAYHLQTTCPDRTYVILEGRDAIGGTW
                     DLFRYPGIRSDSDMYTLGFPFRPWPGDRSIGDGASILAYIRDTAAHYGIDRNIRFGQR
                     VEFAEWSSERSEWTVHTRDAHTGEQRQIACQFLFGCAGYYDYSKGYTPEFEGTSDFRG
                     QIVHPQFWTDDVDYTNKRVVVIGSGATAVTLVPAL"
BASE COUNT         2471 a         4600 c         4769 g         2423 t
ORIGIN      
        1 gaagcgtgcc gtcgagctcg cgtgtgccca cgtgggcacg aatgtgcccc tcaccgcgct
       61 catcaaagag gcactgcgcc actgcggata gtggatgcga gccagaagct cgatcgactg
      121 tgggcccttt ggggactcac gcgcaaagaa cgacttcttg cgaagcttcg acgttgattg
      181 gattacagat cggcggtgcg tcgatcatgt catcatcgtt cggtgcggat cgctcgacag
      241 catcgcgaca attttatcgc cgtgctggtc ttcgttggac cgagcgaagg gagtctgctc
      301 gtcgcgccga agctcacgtc ggcgcccgct gtgccctgct tggccgcctg ttttgtcggg
      361 gctgtcgtcg ctggactggt cgcgcgccgt gtcctgccta ccatgcgtct cagcgcggtc
      421 ttggtctgcg cgtttgtcgc gcagtgcatc gtgttcggag tgcgagcgag gactggcggc
      481 gtggttgcga gttaccaagt accgttcgcg gtcgccggca catgggccgg atgtgttctc
      541 ggcgcagcca tcgcgaaacc gcgagagcgc ggcggtaccg tgtggctcgt ccttgccctc
      601 ggtgcgatcg agctcggact cttcgcccta ttcatgacgg tcgcaacctg ggtgggcttc
      661 tcgcccttcg tgccgatcgt cgtaggagtg atgggaggcg cgatcttgtt cgtcgttctc
      721 gtctcagacc tccagcaatg gcagtttggt gttgcagctg ctgggtcgat cggaacggtg
      781 gtcatcgcga gccaaccatc cgtacccgac ttcggcgatg tcgagttcgt gctcctgttc
      841 gttgcgatca gcatggtcgg cggcatgctc gggcgcaccc ttcgaagtcg catcaaaacg
      901 agatccgaat taccgccggc gcagctgcgc aagtagcgct aggcgaggag ctcgaatacg
      961 gctgttgccc acgcgacgac ggaggcgtga cgcggcgtgg tcgggccgtg gtacgcgacg
     1021 tggtcgagcg cggtgcgcag atgcgcgtgg acgacggcac gctcgtcgag gggcgagccg
     1081 taggccgcca tcgcgatctg agcgaactga gtgccgagcg actgcgcgta gaggaattcg
     1141 ctctcggcgt tgtcgaggcc cgagtcgctg agatcgaaga cgccgatgat gtcgccggaa
     1201 ggggcggcgc acatgttgtg gaagtggagg tcggcgtgga tgacggcatt gatcggcgtg
     1261 agggtagcga gccaatcgcg tgtgcgatga gcggcggcct tggcggcagc ggaggccgcg
     1321 gcggacgcgt cgagcgcggc gatggcggcg tcgacgtcga gcgagcggcg ggaggtcggg
     1381 ccgatggcga cggtttcggc ttccatcgtg tcgacggagc ggcggatccg cgcggcgagg
     1441 tcgccgtagc tggcggcgag gcgttcgccg aacggtgaga gtggggcggc gctgcccgca
     1501 tcgaccaggc gggcgcggac gtccgggtta gcggcgagcg cgggagagta gagtggcgac
     1561 agcgagtctt cgcgatggag ctcgtcgccg gtgaggccgt gcatccgctc gcggacttgg
     1621 acgcggcgga cggcgtcttc gcgcaggacg cgcggggcgg gcacgccgcc gatgcggcgc
     1681 cagcgatcga ggagccaggc ttcttgctgg aggcggcgcc agcctgcgaa gtcgtcggcg
     1741 gcgaatgcga cgagattcgg ggcgagcgcg agccagcggt tgtcgcgacg cgacagctcg
     1801 aatggctcgt cgagcagtgc ggcaaatcgt tcgcggctca ccagctgcga ttatgcagcc
     1861 attggcaggt gctcgagctc ggccacgacg gggaggtgat cgctggcgct ccgcgcgccg
     1921 gcctcggtcc atgcgccacc gcggatcagc tcgaggcggc cgcggtagag gatgtgatcg
     1981 agcgggatga gcggagcggg ggcgggccac gtgcgggcga agcgggtggc tgaggtcagg
     2041 ccgcgacgaa ggactttgta ggcagaggac cagggcgtca tgttgaagtc gcccgcgatg
     2101 acgagtgcct cgccgagcat ctcttgggtg atggcggcga cttgatgggg gcgttcgcgg
     2161 aagacgatcg agaggtgagc gttgaggagc tcgacctcgc cgccgtccca cgcgtgacgc
     2221 gtgatgagag cgcagcgctg ctcgcgatcg tggtggcgag ggaggcgcag ctctcgggat
     2281 tcgagaatag gtaagctagg cgaagagagt gtgcagattc cgtagcgctc cgtgccccgc
     2341 tcgacgcagg ggacgaaccg catgtccatg ccgagcatcg acgccagctc ggaggccatg
     2401 tccatcgcgc cgctccgcgc gcgaccgaca tcgacttctt gcaaggcgac cagatgcggg
     2461 tcggctgaac gaatgacatc ggcaatgcgc gcgaggcgag cgggttttcc gcccgacctc
     2521 acgcgcatgt cgaggccgcg cgccgcatgg atgttgtacg tcatcactcg aagacgctgc
     2581 atgcggcccg ctttctccgc taatcgcggt cgttcttgat gtcgttctcg agggcgtcga
     2641 acttgctgtc cgtgtccttc ttgaactcgt cccacttcgc gtcggcctgg tcaccgatgt
     2701 gatcgacctt cgtcgcgagc tcgttgcggc gctcgcgcag cttggccgcc ttctcgtgcg
     2761 acttggtgtc ggccttcgcc tcgaggcgct ggatgtcagc gtcgatacgt gcgaggcgct
     2821 cgttcacgcg ggtgcggtac tcgacgcgcg cggcggcgag atccgtctgc gccttgacca
     2881 gctcgccctt ttccttgttg acgtccttct gctcgtcgcg gacgtcttcg agcttgtcct
     2941 tgacgtcctt ctgctcgtca cggacgtctt cgcgctggtc cttgacgttg tcctgcgcct
     3001 tctcgaattt atcctgcgcc ttttcctcgg cgctcttctt acaggcggtg ccacccagga
     3061 gggcggccga caggatcgaa acccagaaca tgcttcgcat aagaaaaatc tcctttgtgc
     3121 tctggagtga gtgcaacggg ggtgcccgcg aagcgccgcg aaaatgcaat tctgagcggg
     3181 atcgggcgcg accagagcgc tcggtctcga cgagccgcgt gcgcgaactg tcagcaggcg
     3241 atgacgtttg acgacagtgc tagacttgtt gtcgcgtgat cggagaggtc atcgggaact
     3301 atcgggtggt cgccgagctc ggcaagggcg gcatgggcgt tgtctaccgc gcggagcata
     3361 cgcagctcgg ccggccggca gcgctcaaga tgctcttgcc gcagttctcg agcgaccaag
     3421 gcatcgtcca gcgcttcttc aacgaggcgc gcgcggcatc cgcgatcgat catccaggca
     3481 tcgtcgagat ctacgatttc ggcacgcatc gggatggccg cgcgtacatc gtgatggcgc
     3541 tgctcaaagg cgaaagcctc gagcatcgac tcatcagcgg accggtctcg ccgcaggacg
     3601 gcgccacgat cctcgcgcag gtggcggctg cactcgcagc ggcgcatcag cgcggcatcg
     3661 ttcatcgcga tctgaagccc gacaacatct tcctcgtgcc gaacgagctc gtgccgggcg
     3721 gcctgcaggt gaagctgctc gacttcggca tcgcgaagct cgccgacgag aaaaccgcgg
     3781 gcttcaagac gcaaaccggc gcgctcatgg gaacgcccgc gtacatgagc cccgagcagt
     3841 gcatgggcaa ggcggatctc gatcaccgca cggacatcta ctcgctcggc tgcatcctgt
     3901 ttcacgtgct gtgcgggcgc ccgccgttca cgagcgatca gggcaccggc atgatgatcg
     3961 cggcgcatat tcgcgatgcg gctcccgatc cacgcacggt aaatccggcg attcctccag
     4021 acctcgctgc gatcatcctg cgctgcctcg agaaggagcc gagcgctcgc tatcaaagcg
     4081 cgaccgagct gcgcaacgcg ctcgtgcaag caggtgcgaa tgcgccgctg tcgaagccgc
     4141 ccgttgcctc gatggggtac gacgcgacga tcgcgccgag catgtcgccg caattgcacg
     4201 cgctcggcac cgcgccgacg tcggcgcctc cgctctcgta tgcgcctcct gcgtcgcatc
     4261 atccgctcgg cactgcaccg acgacgctcg gtggcgccgc cgcgcagatg actcccgttc
     4321 cgacggtcgc gccgtctcat ccaacaccga agaagggcaa gggcgcgctc atcgctgtga
     4381 tcggcggcgt cgccgttctg gccgccgtcg gtgctggcgt cgcgatgacg cgcaaaaccg
     4441 acgacaagcc gcaggtcgtt gcagcgaagg gctcggatgg ctcagccacg atgcccgtcg
     4501 tcgtcgcaac acccgacgcc gcggtcgcgc cgctgccaag cggggccgtc gacaacgaga
     4561 aaccggcgga gggcagcgac gctgttccgg cactcgcacc cgtgaagtgc gccgatggca
     4621 aggtcacgaa cgtcgacacg cgcggtcgct gctgctgggc cgatcaagtg tggtcggaat
     4681 ccaaggccaa gtgtctcggc aagccgacgt gcccaccggg catgaagctc aaaggtgaag
     4741 agtgcacggc cgtcgttgca gcgggcgtcg cgccgccacc acacggcggc catcccgtcg
     4801 tcgattcgac gccgccgcca ccgtccgcgg ttccgatctt cacggtcgac gcgaagacgt
     4861 atcagccgca cgaaagcgtc acgatcacgt tcggcagccc tgtcagctcg aagtccggcg
     4921 cgcaagcgtg ggtgacgatc agcgagaagg gcgccgccga gacgtcgtac ggcaactggg
     4981 actacgtcaa agacggcgcg acgaccgccg agctcaaggc gccgcggcgt gccggcgagt
     5041 acgaggtccg cgtccatacc gattacccga cgaagccgta caacgttcgc taccggatcc
     5101 cgatcaccgt caaaggcaaa gacgcgatcg cgatcgacac gagcggtggc accggcagcg
     5161 ccacgacgac gacgccgacg cccgcgatcg cgaccgagac tccggtcgac aagcaaaagt
     5221 tccagctcgc gcgcaagaac gtgaagctcg gcggtgacgc ggtgctccac ttccagacgc
     5281 cgctgcacgc caaagatggc gagaagttct ggatcacgat cgtcgcgaag gacgcgccgc
     5341 ccgacaaata cgagcactac gaatacgtcc cgcaggacgc caagaccgcg aagatcgcag
     5401 tcccgtcgac agccggcgac tacgaagtcc gcctgcacgc gaactatccg acgaagtcga
     5461 cgaacgtggt ccatcgcgct cggctccacg tcgagtaagc tcgggcgcga tgcaaacggt
     5521 gacggtcgac gtagctcgcg atccagagac ctgctggcgc gcgttcgtcg atgtcggcca
     5581 gctgccgcgc tgggtgcctg gcctcaagcg cgccgagatc atcacgcgcg cgaagggcct
     5641 gcccgaagaa gtccacttcg agttcgcacg cgagctcgcg tacacgctcg tctactcgta
     5701 cgacaaggaa gagcgcgtcg tccgctggca acccaagctc ggcaagaatg ccggcgtcag
     5761 cgggttcgtg cggttcgaag cgatcgagaa cgggtggaca agaattacct atgggttgga
     5821 acagggagac gcccgaggcc ctgcggaacg cgagctgggc gacctgcagc gcctcgcgga
     5881 agcgttccgg tctcacgtgc agcgttagat ctgagtttgt taggtcagct cacgtgaagc
     5941 gcggatgacg gaaccgcgat ctatgatgat actcgttcgc tgcgatggtg accgagcaag
     6001 ccgtaccgtc cgggaacgtc acaacagcac gagccggtct cgccgatcac gcggcggcgg
     6061 tgctcgatgc ggtggccgat ggcattacgg tcgaggatgc ggccggcaac attgtctatg
     6121 cgaacactgc ggccgccgag ctccgcgacc tgagccggct ggacctgttc tctaccgatg
     6181 gtgcgacgct gtctccacgc gagcttccgt cgcgatacgt gcttgcgggc gagccatcgc
     6241 gcgagctcgt cgtgaagttt cgcgtgaaag acaccggcgt cgaacgttgg tcgctcgttc
     6301 gcagcaacga gatgcgcggt ccagcgggcg aacgctgggc ggtgaacacg ttccgcgaca
     6361 tcaccgcgca aaaggccgcc gaagatgtgg cgcgcgagaa tggtcgccgg ctcgagctcg
     6421 ccctcgctgc gggcaagatg ggcgtcttcg agtgggacat cgccgccaat cgcgtttact
     6481 ggtcgcccga gctcgaagcg atccacggca tggcgccggg cacgttcgac ggcaccgtcg
     6541 agatgtaccg ccgcgacatg catcccgtcg atcgcgagcg cgtcatcgca gccacgaagc
     6601 gcacgctcga gcgtaaggcg ccgcactatc tcaactaccg gatcctgcgc cccgacggca
     6661 cgacgcgctg gatcgaagcg cacggccagc tcgagctcga caccgcgggc aatcctgttc
     6721 ggcttgtcgg catcagccgc gacatcaccg agcaccacga gggagtgcgc gcacggatcg
     6781 acgcaatcct cgacagcatc acggatccgt tcacgatcct cgatcgcgat ctacgcgtcg
     6841 tgcacgtcaa caagacggca gcgcgtcttt cgggacgttc tcccgaagag cttgccttca
     6901 agcacctcga agagatcagt cctacggcga aggagtcgcc gttctggccg gcgtatcaga
     6961 aggtgctcga gaccaaagca ccgcttcacg tcgaggagta catcgcggcg atggatcgct
     7021 ggttcgaagc gaacatctat ccaatcgacg acggtatcgg catctaccac cgcgacgtga
     7081 cgatgcgaaa gcgcgctctc gagctgacga cgcgcctcgc tcggcacagc acgctgcgcg
     7141 ccgacgtcgc cggagcgctc gccgaggagc gcgatgttcc caagatgctg cagcgctgct
     7201 gccagctgct cgtcgagcac ctcgacgtcg cgttcgcgcg cgtgtggacc gtggacgacg
     7261 tgggcaccac gctcatgctc caggcgagcg ccggcatgta cacgcatacg aacggtccgc
     7321 atcgcgcggt cccggtcggc aaattcaaga tcgggcgcat cgccgcgtcg cgcaaaccgc
     7381 acctgacgaa cgacgtcgcg cacgatacgc agatcggcga tccgcagtgg gccgcgcgcg
     7441 agcagatggt ctcgtttgct ggttatccgc tcctcgtcga cggcaagctc gtcggtgtca
     7501 tggccatgtt tgcgaaacat gcgatgcccg atgacacgat gagcgcactc ggcggaatcg
     7561 ccgacgtgat cgcgcaaggc gtcgtgcgcc gccacaccga gctcgagctc gaacagaagg
     7621 tcgccgagct cgctcgctcc aacgcagacc tcgaacagtt cgcgtacgtc gcatcacatg
     7681 acctgcagga gccgctgcgc atggtcgcga gctacaacca gctgctcgca cggcgctaca
     7741 agggcaagct cggcgaggac gccgacgagt tcatcgcctt taccgtcgaa ggcgtcacgc
     7801 ggatgcagcg gctcatcaat gacctgctcg catactcgcg cgtcggcacc cgcggcagcg
     7861 accgcgtcga ggtcgacatg gagaagctgc tgcgaggcgc gctgcagaat ctcgagcgcg
     7921 ctctcatcga ggccgacgcg caggtgacgc acgatccgct tccgacgatc acggccgatg
     7981 aaggccagat gctgcagctc gtgcagaacc tcgtcggcaa tgcggtcaag ttccacggcg
     8041 atgcgaagcc ctcgattcac atcggtgtcg tgaacgacgc gaagggtgtc acgttctcgg
     8101 tgcgcgataa cggcatcgga atcgatccgc agtacttcga acggatcttc gtcatctttc
     8161 agcggctcaa cccacgggag aaatatccgg gcaccggtat cggtctggct atttgcaaga
     8221 agatcgtgga acgccatggc ggtcggattt gggtggaatc cgcgccgggc gccgggtcga
     8281 cgatcttctt cacgattcct caccagcgga gagcgcaatg acaggtaagc cgatcgaaat
     8341 cctgctcgtc gaagacaacc ctgccgacgt tcggctcacg caggaagcgc tcaaagaggg
     8401 aaaggtccgc aacaacctcc agattgcgcg tgacggcgtc gaggcgctcg agctcttgct
     8461 tcgaaaggac gcggtgcgtc cggatctgat cctcctcgac ctcaacctgc ctcgcaaaga
     8521 cggacgcgag gtgctggccg agatcaagaa tcacccggag ctcaagacga ttcccgtcgt
     8581 cgtgctcacg acgtcctcgg cggaagcgga catcctgaag agctacaacc tctacgccaa
     8641 ttgctacatc acgaagcccg ttgacctcga tcagttcgtg caggtcatca aatcgatcga
     8701 tgacttctgg ctgacggtcg tgaggcttcc cacggacaag cgatgaagat cctcctcgtc
     8761 gaagacaacc ttgcagatgc gcggctgttc gccgagctgc ttgcggaggt gccggggcgg
     8821 ccgtttcagc tgacgagcgt cgcgtcgttc tcgtccgcac tggccgaggt cggcgggcac
     8881 gacatcgtgt tcctggatct atcgttgccg gatgctcacg gcatcgacac ggtcacgcgc
     8941 atggtcgaag cggcgcgcga gctgccgatc gtggtgctaa ccggaaccga cgacgatcgc
     9001 gtcgcgaccg aggcgatgaa gctcggcgcg caggactacc tgctcaaggc ggagatcacg
     9061 ccgtcgcaga tcgcgcgtag cgcacgctat gcgatcgagc gcaagcacgc cgagcatcac
     9121 gcgaagcgcg cccaccaggc cgacgaggcg gcacgccgcg cccgtttcct ctccagcatc
     9181 tcgggaacga tcacgtcgtc actcgagctt gcgaccacgc tgcctgacgt cgctcgcctg
     9241 ctcctgccga tgctcggcga cgcctgtgca atcgatctat tgcgcgagga tgcgcgcgta
     9301 gaccgcgtcg cacgtgcggg cgaagccgag cggctggaac ggatcgcaat gctcggcagc
     9361 cgcgtcatcg aagagcgagc cccgcttgcg gtgaacgagc ccggcggtga gtcggtgctg
     9421 tgtctgcccc tgatgggccg tggccgcgtc gtgggtgcgt tgacgttcgt catggcagct
     9481 ccgcgcatgt tcgacgagga gctgcgccgt cttgccgatg aagccgcgga gcgcatcgcg
     9541 atgggcatcg acaacgcgtc gctctacgag gccgcgcagc gcgcgatccg tggccgcgac
     9601 gagctgctcg ccgtcgtctc gcacgacctg cgcaaccctc tcaacgtcgt ggcgctcgcg
     9661 ctgcagagcg tcgcgcacga tccgatgacg gttgcgagtg cattgcctcg cgcgttgcgc
     9721 ggtgtcgatc gcatgcagcg gttgatcgaa gacctcctcg atgttgctcg catcgacacc
     9781 ggcacactgg ggctcgagct tcgccccgtg aacgtcaccg cgctcgtcga cgaagtcgtc
     9841 gaacagcacc gccagctcgc cgccgagaaa aagatctcgc tgaagggcga gacgatgagc
     9901 gagcacatcg gcgcgcgcgc ggaccgtcat cgcctcctgc aggtgctcgc gaacttgctc
     9961 tcgaatgcgt tcaagttcac gccgcccggc ggaaccatca agctaggtgt cgaggtgcgc
    10021 ggcgagcgcg tcggaatctc cgtctccgat acaggaccgg gaattccggc tgagcacctc
    10081 ggacatatct tcgaccgatt ttggcaaccg gagcgtcgcc gcgatggcgt cggcctcggt
    10141 ctcgcgatcg tcaaaggcat cgtcgatgcg cacggcggaa cgatcgaagt cgacagcaag
    10201 cccggcatcg gaacgacgtt ccgcgtgttc ctcgatggtg cggtgctgcg gccaacgggc
    10261 gtcgtagcgg tcgtcgcgta ggttcagcga taacgccgat ccgggaggcg atcgcgctgc
    10321 tctcggattt cttcgtgcgc gttgacgtca ccatctggat cgatgccttc gcccgctacg
    10381 tgcgacagcg gcacaggatc gacgtcggca attcccgact tcgtcgcaac atctcgcatg
    10441 tcgtcgtcga tttctttatc gagcttgtgc tcgtcctccc aggcgttgcc gcgtttacgc
    10501 agcatcgaga gtagtctgtc cgcgatcttc atacggatca gacgagcaat gctcaggcca
    10561 acggtgccag cagatcatcg agctcgcgtt ccgtgagcga cgtcgacgac gctttgccgt
    10621 tgtcgaggat cgcatttgcg agcatgcgct tgtggcgctg cagagagagc atgcgctcct
    10681 cgacggagcc ggccgcgatc aggtcgtgca cgaacacggg cttcgtctgg ccgatgcgat
    10741 gcgcgcggtc ggttgcctgc gcctgcgcgg ccgggttcca ccacggatcg tagtggatca
    10801 cggtgtctgc gccgacgagg ttcagcccgg cgccgcccgc tttgagcgag atgaggaaca
    10861 cgtccgcgcg gccctgggta aacgcatcga tcggcttttg gcgatccggc gtgttgccgg
    10921 tcagcgtgac gtggccgatg ccgcgcgcga gcagcgattc ggagatgagg cccagcatgc
    10981 gcgcgaactg cgagaagatc aggatgcggc ggccatctgc cagctgtgac gtgacgagct
    11041 cgaggagcac gtcgagcttc gcgctgccgc tgatctcgcg tgccgcgtcg acggagacga
    11101 gccgcggatc gcagcagact tggcggagct tgagcagcgc gtcgaggatc gcgatctgcg
    11161 agccggcgaa gccgcgctgg cgaatgtgcg cacggacgtc ggcgtgtgcc gccacgcgaa
    11221 tgctctcgta gagctcgcgc tgcgcgcccg acagctcgac agcgcgaacc agctgcgtct
    11281 tcgcgggaag ctccggtgcg accgcgtctt tcgtgcggcg caggatgtac ggcctcacgc
    11341 gatcgcgcag cgcttcgagc cggactttgt cgccgcggtc ctcgatgggc tggcggaact
    11401 ggagcgtgaa ctcgtccttc gcgccgagca ggcccggatt gatgaaatcg aacagcgacc
    11461 acagctcgcc gagatgattc tcgatcggcg taccagtgag gcagatgcgg aagcgcgcat
    11521 cgagcacgcg gacggcttcg ctcgactgcg ccgatccgtt cttgatcgcg tgcgcttcgt
    11581 cgaggacgag caggtgcaga ggcgtcgttg cgagctcgtc gcgatcgcga gccacgagcg
    11641 gataggtcgt gatgatgatg tcgtggcccg cgagcgtcgt cttcttgcga tcgggaccgt
    11701 gatacacggc gaccgacagc gacggcgcga acttcgcgat ctcgcgctgc cagttgccga
    11761 cgagcgacgt caacgtcacg atcatcgcgg gacgatcgag gcggccggcc tctttctcga
    11821 gcagcacgtg cgcgatcgtc tgcagcgtct tgccgaggcc catgtcgtcg gcgaggacgc
    11881 ctccagcgcc gtgctcgcgc aggttctgca gccacgcgac accatcgcgc tgatattcac
    11941 gcaggtccgc gcgcagcgtc gccggcgtcg tcatggcgcc ggtcttgcga gggcccatcg
    12001 cgagcgcgta tgcctgctcg cggaacttcg tgtcgccgat ccaacggaaa ggccgcgtgc
    12061 cgtcgtgcac ggctgcgcat agctcggcga cgagcggcgc gcggaccgcc ggcgctcgca
    12121 ccttgccgtt ctcgcgatac agctccatga gcaccttcgc gagcagcttc aatcgcgcag
    12181 gcggcaccgg cagccaacgg cggtcaccga cggggacagc gacgcagcgc cgcgcgctct
    12241 tcgcgagtgc accgagcccg ctcgcgctgt cgagcatctc gagcaacgcg ggcaggagat
    12301 tcacgcgctg gccatcgatc tcgacgccga gctcgagccc gaaccaatcc ggcttctcgc
    12361 gatcgggttc ggccgcgacg tagagcgggg catcaccgcc gaccacctgc cacggatact
    12421 cgctatcgat ctcgacgcgc cagccgagcc cccgcagctg cgggatcgag tacgcggcaa
    12481 acgcgcagag cgcatgcacg tcgccgtcga cggcaacgac gtaatcgatc acgctgcctg
    12541 gcgtcaccgc gcagtcttcg aggtgcgtca gctcgatcgc gccgaggctc tctagcacgc
    12601 ggcacgcctg cgattccgca tcgcgatcgc ggccggccca ccgcacgtca tccgagcgca
    12661 cgcgccgatc gccgtacgcg aagtcgaggc gcagcatcgc cgtttcgacc tcgtggccgt
    12721 cgtcgacgaa cagcgtatcc gcagcgacgc gcaagcacgg tacgcagcgg gcgggcgcca
    12781 ctccctcgtt ggcgccagga acaaacgaca tctgacgatt cacactgccg atgtcggatc
    12841 gatccgtcca gtttccggat catcgtcgat actgatcatc tggatcgtta gagatctccc
    12901 gacgtgattg ctgtccggac cggttgacgg atcagaaagt cccgctgacg ccgaggctca
    12961 ggccgccacg cgcgggcttg atcgacgcgc tccacgtcgg agccgtccgc ttttcgtact
    13021 tcgcgaggta cttcgcgtcg aggaccatcg cggtcacgcc gcccgccatg ccgccgagga
    13081 tcatgccgac gagcgagcca tcgccgccgc agctgctatg ggacggcccg tcgtacccgc
    13141 tgacggactc gacgcagatc gcatcgtcgg ggccgacgtt gtagccgacg tacgcgccga
    13201 gcagcggcag gccggcacgc aggccgaggc tctgcagcgc cgtgaccccg cggccgtgcg
    13261 cgatgtgaac gacgggcgcg gcgaacatgt agccgaccat gccccagccc gtgagctcct
    13321 cgttatcgcc gagaggacca aggaagatcg cgccgaccgc gaggccgtcc gcgatgagga
    13381 tgtcgcggcg gtagctcacg gtgacggtct gcggctcggg cgtcgtcgtg ctcggggccg
    13441 cttcttcgac cggccagctc atggtctcgc ctggcgcctg ggccgcggcg acggaggtca
    13501 gggcggtgag gacagcggca acgacgacgg ttcgcatggt gagcgtgatt agcagcgccc
    13561 atgccgtcgc aagtgcgcgg aatcggccgg gctgcagcgc gggccattcg gttggttttc
    13621 cgggcctcct cgaacctcgg acatccatgt cgcgatcgag gcgagtagac tgccggcgtg
    13681 gataacgtcg atgtcgtcat catcggtgcc ggcatttcgg gcatcgatgc cgcctatcac
    13741 ctgcagacga cgtgtcccga tcgcacgtac gtgatcctcg agggacgcga cgcgatcggc
    13801 gggacctggg acctgttccg ctatccgggc atccgctcgg actcggatat gtacacgctc
    13861 ggctttccgt tccggccatg gcccggcgac cgctcgatcg gcgatggcgc gtcgatcctc
    13921 gcgtacatcc gtgacaccgc cgcgcactac ggcatcgatc gcaacatccg cttcggtcag
    13981 cgcgtcgaat tcgcggaatg gtcgagcgag cgatccgagt ggacggtcca cacgcgcgac
    14041 gcgcacacgg gcgagcagcg ccagatcgcg tgtcaattct tgttcggctg cgcgggctac
    14101 tacgactaca gcaaaggcta cacgcccgaa ttcgagggca cgtcggactt tcgcggccag
    14161 atcgtgcatc cgcagttctg gaccgatgac gtcgactaca cgaacaagcg cgtcgtcgtg
    14221 atcggcagcg gcgcgaccgc ggtgacgctc gtgcctgcgc tcg
//
LOCUS       DATLBZ010000214         5087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1705825, whole genome shotgun sequence.
ACCESSION   DATLBZ010000214 DATLBZ010000000
VERSION     DATLBZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705825"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VMZ53_11350"
     CDS             complement(<1..524)
                     /locus_tag="VMZ53_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUS29103.1"
                     /translation="MARVLRLPDKGRLLVCTDLQGCMRDFDKMVEVFEQALSTYEGDA
                     HLLFTGDLIHGPHIEPEDLPDFLGEYYRDASGEVMIAYAGLAAQYPGKVHALLGNHEH
                     GHIGGPHTAKFAADEVALLEHILGPAGTARMRGIIHTFYLAAVSKCGAVFTHGAPAAQ
                     CESVADVEAADLSGA"
     gene            567..836
                     /locus_tag="VMZ53_11355"
     CDS             567..836
                     /locus_tag="VMZ53_11355"
                     /inference="COORDINATES: protein motif:HMM:NF011013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HUS29104.1"
                     /translation="MRRIRAIVSGRVQGVSYRASTADKARQLGLAGWVKNLPDGSVEL
                     EAEGSPDQVAALVTWCHHGPPAARVDNVTVEELSPTGGDDFDVRY"
     gene            complement(833..2266)
                     /locus_tag="VMZ53_11360"
     CDS             complement(833..2266)
                     /locus_tag="VMZ53_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29105.1"
                     /translation="MRVWLLVAVSLGACSPSVPEQQDAPITILSDGPNGPDPNDGARS
                     GTRLKLTWYQLADGTRQWDGFYDAQRKENCYIFDNWIDGKAYCTPDYDGSIVYSNATC
                     TTKVAEIYKDPTCPTTPQPYTLEYSYTPCESHPAHLYVRGAKVGLASYYYKSSDGTCG
                     GPYTTSTSYDYYAIGAEVPPSELVQVQLGAPIGNGRLSERFWQSADGMQRQATVHDAA
                     LGTSCYPGATGCQPSARYVSYEENATCTTPKLSIPKTCAVPKFAVYYPNNACYSDPGK
                     YYTVGAATTGTPLYYFNGSTCTAQTPSTTNNYYKMGTAVTLAPTTRQIGTAGQRIQLI
                     HYTNDEGLNYRSSTLYDAQKGAECYPTKLPDGTIRCIVYGGYVDTFYRDTTCTQPIDL
                     IQVSTGGASCTPPPAPKYARKYITPTPGSCSYNTAVYNVGAAYTATVYENNGTCVAHS
                     TADNKLYSLGAVIPVTDFITASITIDP"
     gene            complement(2286..4010)
                     /locus_tag="VMZ53_11365"
     CDS             complement(2286..4010)
                     /locus_tag="VMZ53_11365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS29106.1"
                     /translation="MAIDVSVVRDLTALRALESEWKALAGAGPGALFRGPDWLVPWWL
                     AYHNTLGAELHVLVGRATEADATGTQVGDIVCLAPFYRRTVKVALLDTRELRMMGDAG
                     PRPPSLDLLARKGWEDRAGAALAKALIDDAASWDLIDLEPLADPSRVRANLVQRIAPA
                     GFTVESSMSGGGASRVALALAPVDASDATGVIATHGDDLIALRKGMSVLRRLSRLEWA
                     ERDEPSPLADNEAASLLEEVALELGKQGRVRLAHLDDAAGEAVAAALVVDDGDRAVVL
                     AMAVDPQVPPKRAARLLHAEALSARSRGCIALDVVTGAVEYPMPQLPTSKQPALSVRI
                     WGHSTTASVGRTMTHVAKRARRARETPSVAAAQARAAWTKIRTAAANVAQYDRYGLYR
                     GQLWTRGIEPPPGLELVAFAEADFDKLDEARRAEIIDQLQLDEETARRFWKRGDNTVL
                     ATLGVRPAGILWTARGPVDVPELGRTLRMSKYDAYIHSVFVAPAARGRAVAPVMLEHM
                     AKELRASDAYRSWALISSHNQASIRAFVKASFTPVCDVIHAKMATVDRVVCRPPDPEA
                     EELLGLDT"
     gene            complement(4166..>5087)
                     /locus_tag="VMZ53_11370"
     CDS             complement(4166..>5087)
                     /locus_tag="VMZ53_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS29107.1"
                     /translation="NERNLEKRIALAASLPDWLAKRFVADWGAEAEPLALALNQRAPM
                     TIRANTLVTSRDALAKELASEKLDTHPGAWSDSALVVDSRTNLFASAAFKRGAMEAQD
                     EGSQLLAELATPGKLIVDYCAGAGGKTLAIAARLANRGRIIATDIDDKKLEELRKRAR
                     RAGVSNVQAVALDNGGWPRELDSVRGKADVVFVDAPCSGTGALRRNPEARWRMREQDI
                     ESFQQKQVAIMQQAIELAAPGARIVYATCSLLNAENDEVARRLTGVDLVDLGTVLANR
                     AELSREGAFTVTPHVHGTDGFYARIMTRAR"
BASE COUNT          798 a         1667 c         1727 g          895 t
ORIGIN      
        1 gcacccgaca ggtccgcggc ctcgacatcc gcgaccgact cgcactgcgc cgccggcgca
       61 ccatgcgtga agaccgcgcc gcacttgctg acggccgcca gatagaacgt gtggatgatg
      121 ccgcgcatgc gcgccgtccc ggcgggaccg aggatgtgct cgagaagcgc gacctcgtcg
      181 gcggcgaatt tcgcggtgtg cggcccgccg atgtgaccgt gctcgtgatt gccgagcagc
      241 gcgtgaacct tgcccggata ctgcgcagcc aatccagcgt acgcgatcat cacttcgccg
      301 gacgcgtctc gatagtactc gccgaggaag tccggcaaat cctccggctc gatgtgcggg
      361 ccgtgaatca ggtcgcccgt gaagagcagg tgcgcatcgc cttcgtacgt gctgagcgct
      421 tgctcgaaga cctcgaccat cttgtcgaag tcgcgcatgc atccctgcag atccgtgcac
      481 acgagcagcc gacctttgtc gggtagcctg agcacgcgag ccatcgcgac gaacctaaca
      541 cgccagtcat gtgggagagt tcgtccatgc gccgcatccg cgcgatcgtc tcgggccgcg
      601 tccagggcgt ctcgtatcgc gcatccaccg cagacaaagc gcggcagctc ggcctcgccg
      661 gctgggtgaa gaacctcccc gacggaagcg tcgagctcga agcagaaggc tcgccggacc
      721 aagtcgccgc gctcgtgacc tggtgccacc acggcccacc cgccgcgcgt gtcgacaacg
      781 tcacggtcga ggagctgtcg ccgacgggcg gcgatgactt cgacgttcgc tattaaggat
      841 cgatcgtgat cgacgccgtg atgaagtcgg tcacggggat gaccgcaccg aggctgtaga
      901 gcttgttgtc cgcggtcgag tgcgcgacgc acgtgccgtt gttctcgtag acagtggccg
      961 tgtacgcagc acccacgttg tagaccgcgg tgttgtacga gcagctgccc ggtgtcggcg
     1021 tgatgtactt gcgcgcgtac ttcggtgcag gaggcggcgt gcacgaagcg ccacccgtcg
     1081 agacctggat gagatcgatc ggctgcgtgc acgtcgtgtc tcggtagaac gtgtcgacgt
     1141 agccgccgta cacgatgcag cggatcgtcc cgtccggcag cttcgtcgga taacactcgg
     1201 cgcccttctg cgcgtcgtac agcgtcgagc tgcgatagtt gaggccttcg tcattcgtgt
     1261 agtgaatgag ctggatccgt tggccggccg ttccgatctg tcgcgtcgtt ggagcgagcg
     1321 tgaccgcggt gcccatcttg taatagttgt tggtcgtcga cggtgtctgc gcggtgcacg
     1381 tcgagccatt gaagtagtag agcggcgtgc ccgtcgtcgc ggcgccgacc gtgtagtact
     1441 tgccgggatc cgagtagcag gcgttgttcg ggtaatagac cgcgaacttc ggtaccgcgc
     1501 acgtcttcgg gatcgagagc ttcggcgtcg tgcacgtcgc gttctcttcg tagctgacgt
     1561 agcgcgcgct cggttggcag cccgtcgcgc cgggatagca gctcgtgccg agcgctgcgt
     1621 cgtgcacggt ggcctggcgc tgcatgccgt cggcgctctg ccaaaaccgt tcgctcaggc
     1681 ggccattgcc gatcggcgcg ccgagctgca cttgcacgag ctccgacggc gggacttcag
     1741 cgccgatcgc gtagtagtcg tagctcgtcg acgtcgtgta cggaccgccg cacgtgccat
     1801 cgctgctctt gtagtagtag ctcgcgaggc cgaccttggc gccgcgcacg tagagatgtg
     1861 cgggatgcga ctcgcacggc gtgtagctgt actcgagcgt gtacggctgc ggcgtcgtcg
     1921 ggcacgtcgg atctttgtag atctcggcga ccttcgtcgt gcacgtcgcg ttcgagtaga
     1981 cgatcgagcc gtcgtagtca ggcgtgcagt acgccttgcc gtcgatccag ttgtcgaaga
     2041 tgtagcaatt ctctttgcgc tgggcgtcgt agaagccatc ccactgccgg gtgccgtcag
     2101 cgagctggta ccacgtgagc ttcagccgcg tgcccgagcg agcgccgtcg ttcggatcgg
     2161 gaccgttggg gccgtcgctc aagatcgtaa tcggcgcgtc ttgctgctcc ggcacgctcg
     2221 ggctgcaggc cccgagactt accgcgacaa ggagccagac ccgcaccgcc tgaggatacc
     2281 gctccttagg tatcgagtcc gagcaattcc tctgcttcgg ggtcaggtgg ccgacaaacc
     2341 acgcgatcta cggtcgccat cttggcgtgg atgacgtcac aaaccggcgt gaacgacgcc
     2401 ttcacgaacg cgcgaatcga cgcttggttg tgcgacgaga tgagcgccca cgagcgatag
     2461 gcatcgctcg cgcgcagctc cttggccatg tgctcgagca tcaccggcgc gacagcacga
     2521 ccgcgggccg caggcgcgac gaacacgctg tggatgtacg cgtcgtattt cgacatgcga
     2581 agtgtgcggc cgagctcggg cacgtcgacg ggacctcgcg cggtccacaa gatgccggcg
     2641 ggacgcacgc ccagcgtcgc gagcacggtg ttgtcgccgc gcttccagaa gcgacgcgcg
     2701 gtctcctcgt cgagctggag ctgatcgatg atctcggcgc ggcgcgcttc gtcgagcttg
     2761 tcgaagtccg cttcggcgaa tgcgacgagc tcgaggccgg gcggcggctc gatgccgcgc
     2821 gtccacagct gcccgcggta gaggccgtag cgatcgtatt gcgcgacgtt ggcagcggcg
     2881 gtgcggatct tcgtccacgc ggcgcgcgct tgagcagcag cgaccgacgg agtctcgcga
     2941 gcgcggcgtg cgcgtttcgc gacgtgcgtc atcgtgcggc cgacggatgc cgtcgtgctg
     3001 tgaccccaga tgcggacgga gagcgccggc tgcttcgagg tcggcagctg cggcatcgga
     3061 tactcgacag cgccggtgac gacgtcgagc gcgatgcagc cgcgcgagcg tgcggagaga
     3121 gcttcggcgt gaagcaggcg agcggcgcgc ttcggcggca cctgcggatc gacggccatt
     3181 gcgagcacga ccgcgcgatc accatcgtcg acgacgagcg cagcggcgac ggcttcaccc
     3241 gccgcatcgt cgagatgcgc gagccgaacg cggccttgct tgccgagctc gagcgcgact
     3301 tcttcgagca gcgatgcagc ttcgttgtcg gcgagcggcg agggctcgtc gcgctctgcc
     3361 cactcgaggc gcgagagacg gcgcagcacg ctcatgcctt tgcgcagcgc gatcagatcg
     3421 tcgccgtgcg ttgcgatgac gccggttgcg tcgctcgcgt ccaccggtgc gagggcgagt
     3481 gcgacgcggc tcgcgccgcc gccggacatc gagctctcga cggtgaaacc ggcgggcgcg
     3541 atacgctgca cgaggttggc gcgaacgcgc gacggatcgg cgagcggctc gagatcgatc
     3601 agatcccagc tcgccgcgtc atcgatgagc gcctttgcca aagcagctcc ggcgcggtct
     3661 tcccagcctt tgcgcgcgag gagatcgagc gacggcgggc gagggccggc atcgcccatc
     3721 atgcgcagct cgcgcgtgtc gaggagcgcc accttcacgg tgcggcgata gaacggagcg
     3781 aggcagacga tgtcgccgac ctgcgtgccc gtggcgtctg cttcggtcgc gcgaccgacg
     3841 agcacgtgga gctcggcgcc gagcgtgttg tgatacgcga gccaccacgg cacgagccaa
     3901 tcgggtccgc gaaacaacgc gccgggcccc gcacccgcga gtgctttcca ttcggattcg
     3961 agagcgcgaa gcgccgtcag gtcgcgcacc acgctgacat cgatcgccat tcgcttctcg
     4021 aagctatcac ggcgtgagtc acccggcgcg ttgcggttgg caacgtcacg cgttgacgaa
     4081 gtcgtcgtca acgcagccaa atccggggct gcggggtcca tacttggggg aatgcatggt
     4141 ccattccgtt ggaccgggca aggcgctatc gagcccgggt catgatccgc gcgtagaacc
     4201 cgtcggtgcc atggacatgc ggcgtcaccg tgaaggcgcc ctcccgcgag agctcggcgc
     4261 gattcgcaag gacagttccg agatctacga gatcgacccc ggtcagccgg cgcgcaacct
     4321 cgtcgttttc cgcgttcaac agcgagcacg tcgcgtacac gattcgcgca cccggcgcgg
     4381 caagctcgat cgcctgctgc atgatcgcga cctgcttctg ctgaaagctt tcgatgtcct
     4441 gctcgcgcat ccgccaccgc gcctcgggat tgcggcgcag cgccccggtt cccgagcacg
     4501 gcgcatcgac gaacacgacg tcggctttgc cccgcacgct gtcgagctcg cgaggccacc
     4561 cgccgttgtc gagtgcgacg gcctgcacgt tcgagactcc cgcacgccgc gcgcgcttgc
     4621 gcagctcctc gagcttcttg tcatcgatgt cggtcgcgat gatgcgtccg cgattcgcta
     4681 ggcgcgctgc gatcgcgagc gtcttgccgc ccgcgcccgc gcagtagtcg acgatgagct
     4741 tgcccggtgt cgcgagctcc gcgagcagct ggctgccttc gtcttgcgct tccatcgcgc
     4801 cgcgtttgaa cgccgccgac gcgaacagat tcgtgcgcga gtcgacgaca agcgcgctgt
     4861 cgctccacgc tcccggatgc gtgtcgagct tctcgctcgc gagctccttg gcaagcgcgt
     4921 cgcgcgacgt cacgagcgtg ttcgcgcgaa tcgtcatcgg cgcgcgttga ttgagcgcga
     4981 gcgcgagcgg ttcggcttcc gcgccccaat cggcgacgaa tcgcttcgcg agccaatcgg
     5041 gcaacgacgc agcgagtgcg atccgcttct cgaggttgcg ctcgttc
//
LOCUS       DATLBZ010000215        33921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5432059, whole genome shotgun sequence.
ACCESSION   DATLBZ010000215 DATLBZ010000000
VERSION     DATLBZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 33921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33921
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5432059"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..8385)
                     /locus_tag="VMZ53_11375"
     CDS             complement(<1..8385)
                     /locus_tag="VMZ53_11375"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HUS29108.1"
                     /translation="MWRALARSTGISEELARALWARAEAESGKDPIRTERTFRRLVDE
                     AQELGQLARGLDGDAGLLDPGKWTRVLDLQNGDAKRGDIQQSHDVPGPKSEIDASALD
                     PATRLRQAFEKMVTKGEVIAETITHSDGDSIRDALRKFRNGQVGSTIAGVDNGGLDQI
                     LRIADALISIAEHDRDFRAGKPLPTNLRAELESKLGIDFSDVRVHDDSKAAREASSHG
                     AVAFARGKNVYFADGAYDPSSAEGKRLIAHEVVHVAQQRAGGNAAPGSMSEPGSAVER
                     EADRIANAFAGGPVAGAGAFRVRESAAAGTISRKTEGDSDKKITKWSPRLVGQALDLG
                     SRFVDAYDAGEGKKEFTIPAPLNRLGPLKLTKAQFKPNEAGDKVEQGTLFAKIDSGAL
                     SGTEGHLTVDANEGVSGKLTIPVNAPNMLSIKEVSVEVGAGEISTTVRVKPDFKASKH
                     FAVKETDFEVTISSKAGELELKVIGHTEVEIKNGFADGAGKLDLDLKANKEGVSFNAF
                     VNGEIKVAGLAEAKATIFFNGEKLEMTAGGTVPFNLPGLKGSANIKYEGGKLSLDSKD
                     LHFTWPKLAPINFSEVALGGESKVKAELHLNEAISVDVGGATISLEDSSIKIDGKKVE
                     GHITGGFALNNAGGFKAKAKIGYADEGGFTGEVSVEGGATVNVGPAKVTINEGSKLTV
                     SKTPTALDIAGDISATAKIPALGDLDAHLIAAKGKPIDLTIDAKMDMKKIQKDLRGEL
                     NVKYKRSSGSNAFALSATNVGVQVGPLKDKTIFSSVTGTYENGKVTANLTAASGTEIV
                     VSEGSSVTINSGHITLADKKLDGHLDATGKSGGSEVKATIGWKDGHFDWAAEGNFELG
                     GITNHMLEGSVHAAAGSDGTGNFVSNGPITFGNEKLKDIEITHLEGNKQTNKYVASIQ
                     AEKAINKAVKNVPDVQIESAGAGAKITYDNGKLSFGGMVQISAKYPKSGDPEVSGTLR
                     LEIPEGTTDLHGEIVDITVKKGDFFESKGGKVDLNSGQVDLGKNSTFKIPGVAEGKIN
                     SASFNIKTKEFAADIDVDPKQKALDGVQFNVTATNNSFVAKLRANTPPIALGSFATAT
                     LGPNSQVSITKASGLVADLEASVVAPNVGKGDFHLHYETKTRKFTGNAKVTVDPFAVF
                     DKVDIDVRVDENRQVSTNKPIEVSLAPNYTDQFVASATVEVTNNKFDVKGQVKEIRNL
                     GSVSDAFKEKGAQIHWSQGGGVTIDAGIAMHKVIPQLAEGSDLTLHYANKVFTINGTL
                     KPKTLGPVTFKPESHITANWTSNTKQLIVSGHATADIANLCEAEMTVRANAGGGLAGS
                     FHLDGKIDATKLSEKVPGVKFSSVVATFGVDIGGGAPPALDFGLKAGITSIADFKVAA
                     SLDANYKSGEGFGGTLAVTRAELGPVIADGSIVVEKNKFKEGNLHMMADFPTLKIEGN
                     AKVTPGEMGGLDTAVKLTVTPAKTSAIAEFVQSGEINVDIKKWKVMKAEGILNLVPPS
                     FLPLVDPKIVISYVADGDLSATLKTQFKAPMAKNNEMGNFEAGYSKSKGLFAHIDFPL
                     TVPGFQATTVYGDLDQTSAKVGVKLRPKDDSLVKEAVVELGYIKGSGFFIEGRIKLTP
                     TETLTLDVGLRYSSEGGLQVIGMEPDDKDANNDEHTVGKFGKSISVPLLTVGVAQLAL
                     KFGIEVGAGYRMPKIKFKNPKLEGGLDALDKGGMPSFTFGGSIAMGAYVYMALSVQIV
                     GEIQLLIATASAGIGAEIMARLNLELGADVDGRYQSGQGALITIDPYVAASLDLVASL
                     IATLYASIGWWTIVDKKWTLAQATLAHIDLGRFQPFQPVGLQFGGPGGTKLTRGLSLR
                     EDAFDNVEKGVKEGGQKAADNESNREAREKITPVLQAFKNAAPQFQELPEGWEKGMVA
                     APVDFSSMFPVQDKHWKFYEEHADQAEDMVPELRMSTPTERLAKAVAVTAKKDPTGAG
                     RLVLGWRRAQIAAKGINPDTGVNVIEERVIVQQLIEEKYQKDLKEAQEKQKKQDEDHA
                     AQVAKQAADFGKAEVAHAQVGIEQKTAFDQNVNKIQGEWDKAQEKKAEAGKAAAQEGA
                     QVKPVAQEKAPPPAKPAPPPMPKPLSKPAPIPVPPPIPLPPPAEVLPAVTLPALPADP
                     GVSVKAAGVIPPQKKKAEVQSPGKKDAPQGGAPNPTPGATSNTAAQSAGAGGSPMPQG
                     EGNQGGAPKVGGSTTATPPPGPKVAAGPDGIISQNKTIDGKHKQYDPSFKPAGVVAMP
                     TASMVSALFGDKKGPDKAKPGDGAKPGGPEGAPGGDAAKAGAAKPGAGAVDPSVQKVI
                     DEGKAGQQQQKAALDGEKQKYDKQVDQKNAQTAAETKKLDAAAAEAKKKKEEEKKAAD
                     AKAAAAAAAPKPGDEKDKDKKAKGPIGVKVSLDILGESHTLYIDELTGVAMVASTPTP
                     VSTKIGEAESAIKAAPDSLRTPAYDTVQTTKTAATTLDTTAKKAFAGDESAKSAAASD
                     QQTLSNPLKITWSWLKVAQDPAVTSGSIDKPLLHPYYNTFKGRVALMSMQGKLNVNVA
                     PFTEDIWIKICEAVKKAQPAMTDPAAYSDFAKGWLDMKSPVFKNALKEFDKIGDELAK
                     AGKSAFGQATSFGFWSKDEGRSLAESMSGSLTLETSAIGGLMDGMPTLDGKKAGWDPE
                     MWGALSNAYANAVVPEIVKGKKVKVCVGAGVPAGNIWEAVESVALEHGLKQAGISLES
                     VTTHYAAAAKSKKDRRTLDETKSTGGYKGCLYVGDRPGAIAAANAHFATVPEGNAPVV
                     PAPTPVAAAPAANAAPPAGAAPANA"
     gene            8645..9319
                     /locus_tag="VMZ53_11380"
     CDS             8645..9319
                     /locus_tag="VMZ53_11380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS29109.1"
                     /translation="MSRDEEELERAKEHLRSLQMSLPELIEYLRKLAEIYSRPDIARA
                     LPNAQQEAERIAGLLAYIERYTRVDAQTFGPVDLRAVLEEAVALTRAEIEQRGRVSVA
                     YLEAPLVRGNPRQLGHVFIALLINAAQSLAEGAPHANYVAVELDTNPDGGWARIAIAD
                     SGSGIYPEDVAHIFEPLYTTKRGAGMGIGLAAVREIITGLGGRISVESVPGSGSLFIV
                     ELPPAS"
     gene            9316..10551
                     /locus_tag="VMZ53_11385"
     CDS             9316..10551
                     /locus_tag="VMZ53_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29110.1"
                     /translation="MNKVSERAGSLGLFATANAFADAKLDPDVAPRDPRLKEAAATDL
                     KRLFGQMARRGHDLELVRAGWKMLERRPLERALFAVDVLAQSILPQNEPILLEMRTVA
                     NMLGGARPLGVEVGIDLNKVSASHLTEQHARRPEIAASDTVDLLPLAFEHHLGALAEL
                     HQRGDALISQAGTCESIQAFARLAALARLPTLASVYLDWLVRGVGWRVPVLDLCDTLL
                     DAGVPQKIPGDGIQAGDIPEKEMRDSVEYLVYRTHLSLGDYDRANALLSQNMEQRPRW
                     IGTPSYRLDAVRGHLGALFGHSNDVTLARIEAVCMEDRLWRYGAKVRAIVAAARQPGR
                     ASEMFHAYVGGFGNDYDMTFSVISLAPEQVKRDVARILAREAYYLPHEPAVWKLLAAL
                     AGNDKPVDEVDARLKAQAR"
     gene            10560..11969
                     /gene="mqo"
                     /locus_tag="VMZ53_11390"
     CDS             10560..11969
                     /gene="mqo"
                     /locus_tag="VMZ53_11390"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF003606.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HUS29111.1"
                     /translation="MDAHIDVALVGGGIMSATLATLLRKLDPTLRMVAYERLDHAAAE
                     SSDAWNNAGTGHSGFCELNYTPAKPDGTVECAKAIKIAGQFQQSVELWRSLIAAGELP
                     VESTFLREVPHISFVTGDDVPYLRTRAKTLRAECPLFHDLEYTEDVAKIAEWIPLVME
                     GRAAGTPVAATRMMHGTDVNFGALTRSLINWIDDESGFAIELSREVRDIRRAHPGEGW
                     LIDIKNLATGAEHTVHAKFVFIGAGGYSLKLLEKTGIPEASGYGAFPVSGQWLRCTNR
                     NVIDRHHAKVYGMAEVGAPPMSVPHLDTRWIDGTRELLFGPYAGFTTKFLKEGSWLDL
                     LGSIGIHNMRPVMAAGFDNMNLTKYLVGQALLSEEERVDLLRRYCPTARGDDWELQIA
                     GLRVQIIKSDGHGGGELKFGTEVVTSADGSIAALLGASPGASTAVSIMLELLQRCFPG
                     KWASAEWRSRLSAWIPSLG"
     gene            11979..13175
                     /locus_tag="VMZ53_11395"
     CDS             11979..13175
                     /locus_tag="VMZ53_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29112.1"
                     /translation="MSFAETANRRFLRSVSGHLGELSWEIDADGYQRLQVPPTADLDI
                     LLRLLTRRGLDPALTTLLLDRFANRPLERALIAWDVAAGSLLGSDDGALLERVRAELA
                     PLKKIGTSASVALSRGQVGALIEAVVAVGEKPMSEGSAPQPSLADAATSIATDTAGIE
                     AALAFADRLALAHLPSLSLAFAQILYSRHSLPAALDRIVETALDYERFDSIPAMTESD
                     DRSLQRQTYFGMRVALAQLDAQSAAKMLAEMAAKHPAVADLSHPTLAAVRVELEVVAD
                     RPVDYSMVATLEALAPDGSKWRYGSRVRDELRIEVAPQNSALTLDGFISSFGNDLRMW
                     AQAGYHDEVRTELLALASREIRYQSHDPDAWRSLAVFVEDGSPIELELQERLAAQLDA
                     VLVAVN"
     gene            13179..13748
                     /locus_tag="VMZ53_11400"
     CDS             13179..13748
                     /locus_tag="VMZ53_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29113.1"
                     /translation="MTTNVDFTKCDQPDELLQLAWNAGLDRRTIIREGSDAARLLLAG
                     EKNEIITLFWPVPRAIEAVDRWAGDPRDSAGNAETMRHFASAVVPGCVLGAAVAHWFV
                     SPHLSENNSATAMFGIIFATIVVLGVVFKVLIGASIRRQVARLDESKALEIVLARLRR
                     GMVAKPRLIPVVVKDIRRGFAASASDQAN"
     gene            complement(13749..14873)
                     /locus_tag="VMZ53_11405"
     CDS             complement(13749..14873)
                     /locus_tag="VMZ53_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29114.1"
                     /translation="MAAPSSPDPSCTDGGMLAGMDIWFPEVPSARFVSDQELVACNRL
                     FCQSCRSWIRSLDACALSQPTPIGDEIEQLFIDPRAERSARVTAALGSRTYVCRCTCV
                     DASKRRAARDAHRGWCCGGHPGAATDDALPSPPVASDTELEKMLRQSADGPPAFGDDY
                     PWHFAMRWRPAFVFDHVWPVVSALLTDGDPTVRRRALEIVFAWKPGSAVALGRLRDIA
                     TNHAELYQDSTLKTELCGALASKAVDLPLQGPLVAKLIVGLLNGEPPRDGAETVVAQF
                     EPNAVIQSASRWTEDASDRSCASAAARAMAIYRRDHLLGFLRRLAGWSEANREQILKP
                     LPNALAIPDETLRVILDKVGVPLPATQPTADECRHALGLP"
     gene            complement(14898..15371)
                     /locus_tag="VMZ53_11410"
     CDS             complement(14898..15371)
                     /locus_tag="VMZ53_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29115.1"
                     /translation="MSDDTETCEIAKGPRAVRIVSSADEVEPELVAFVRSSARWIDPT
                     FPQSGDRALGQKGQYTNDEGIECYFTQVEEYDSDGRGVRIVRYMRVLTPLGPHDTSMT
                     VTAIGLANNASLAVRWDFITTEQNLRGKSAAIFISGARKDQCIADFREWFGATTS"
     gene            complement(15385..15657)
                     /locus_tag="VMZ53_11415"
     CDS             complement(15385..15657)
                     /locus_tag="VMZ53_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29116.1"
                     /translation="MTADRVCALGGLLVDGENRLLGRWRQAMTLLTDRMHKPAQGHLI
                     VIAGARVIQAKLTTPAEFRAWMRSRRGFGWVDDSWVRPLETMLVQT"
     gene            15714..16277
                     /locus_tag="VMZ53_11420"
     CDS             15714..16277
                     /locus_tag="VMZ53_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29117.1"
                     /translation="MTFEEALASPGRKVQIPLAKVRDGKRALLILSVGTDGAQQLAHS
                     DVPEAEIDARVADLKARGIDVAATDIDSDATFVWIRKDGGIEVYDSSCKVFEAAGESA
                     TLLDGTSIARADIARVIAYADGYVHRGVKATLKSGTDVNLVAQVSTSSISDPMYTRNE
                     LLFETGWTATIGIAIADWAGAEFDDQV"
     gene            complement(16274..17590)
                     /locus_tag="VMZ53_11425"
     CDS             complement(16274..17590)
                     /locus_tag="VMZ53_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29118.1"
                     /translation="MTDRVCAQGGLLVDGDTRLLTDPCEYYQTGLDLPVGCGRLRCSN
                     CKEWVRAGPPKLGFKKDAPHDLRALHAAPDWSVLPFIESRYALSRQLRLYACTCTYWE
                     AESVDPIDNDHELASDPNVPWRCAGHPAPDLPLTLNGLRIDAATNWAQLVDKVLKGSC
                     PRELDRMLAIGPEPAIWLSWLYGYLQGLSVADALSAAIADRLQDSDPQVVGRVLFFFT
                     RFPRAVGIVKLVEYAEANPLRVALGYPIPEYRSAPTLWGVLIAHAVTERAELAARVDT
                     LIKRLMFIPRASLPHDDLGPAGTVDFERQRSAKLGYDAKTTDWLLKDFSAARTQERID
                     VVGNAIAHSPGLFDDEPTRTFLADKIVELDAACPGRWRQVMTLLTDWIGKPAQGHLVV
                     IAGARIIQAGLTTPAEFRAWIESRRAYGWVDDAWVLPLETMLDEKN"
     gene            complement(17590..25635)
                     /locus_tag="VMZ53_11430"
     CDS             complement(17590..25635)
                     /locus_tag="VMZ53_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29119.1"
                     /translation="MAAATQGSWIEWRVLAADKHVSLEIAGKLWEQAKAAATDPAQRS
                     TLFRKMLEEAAKNPTASPEPGKANLTDQKPPVDPASLDPGKTTQVQAGQEQKKGPPPI
                     PADAKKSGPPPIPADAKAKAADAKDPATPATPPPIPADAQKKDDAEQQLEDFVKEEIA
                     LLKPKGWATATKSEVLKLVRDLGSFELGLQMKTGKYASHPAVATAKTFITDSRTALED
                     QAQKAFTLSILVKTAAPKLDPTGYKEVEALKGEVTPWLTAFEQDAMFVRGVATCNGMI
                     KEVDTALASILSHRDKLLKAAKTAIDAVDVNTPGTAIADEMAKVQIWLDAILLPDPAA
                     LRASVVVAKKLKEEAIQKAVDAKAAADAKAAADAKAAETAKAAETAKSAAPPPPPPAA
                     GGPQPEAKKELTPEEKHALEKEKEHLELHKVEEQLDLAMENALKGWEVFNAGGAVGLS
                     AAVLIAHIAPQIAIPAAIGKAGIMFFTKYMKKRGLKQAKNQLLMLSPTQIQALGDLFD
                     ADLLSFKEAIVKIDKEAKVAKFQAGAGAATAKTEAAPLAVDTVEVGAGLAEMAGVHHW
                     VQHLAHEAAAFAGPVVAGIQLGLTFRGAKSTAEKIKKIKAKIAEIDKALGLPVAPETP
                     ELPEPAGEALVLPPLPAPPQAVTPKTPPQPQANEVAADAAEPIEETTDMAGAPHSLRT
                     DAKTGDIHFHSIPGFAVSKLKSVVDLVPEPAKTKALNAVATAVEATAMVADPALKPGG
                     KASERPAVVKKLAGKLKELAGAIRAFGNTSKLTDADPETAKRVEAENKKQQQLMTPPA
                     PPAIAAPPPPAAKDPASADASASTAAPVIAPPPPPPPPASVSVSPMAGPPTTEAKPPV
                     ANAPAPVTPGAPSPTATPPSPPPPAAPDPAAAKAPVDPAALKKADDDLKAVFRVVDKV
                     PAPIATLGEQERIDESKKDPKTLRAQHFPTFESLHPVMSATAQEKTTQVQAEKSLEPD
                     ALGLKEHFKADVIAGFHADKYGNAKSIEDVMKMVCILERKGEGGYHMRGTPLTAANFN
                     AAVPSDASLARVINTEGAFSGKNFKEGAAQADWLADPANAGQTAAPKGDKLIAWVLKK
                     AKADPSGTIQAYVNPSIVENPSWMFPEPDIKSDAVFSQYCDLLALYANWYPKGHLRMV
                     VKRTAVLAKITSGDKNQKLRKPTVFDGTLSPLWMQRDARDNNWGKTGGGIRETMMRVA
                     WDDIDHAKWQLVEMDPKYQAVLAAQNANTAAHKAGAAPDAANVATVAAHEQGMLGQSQ
                     ANQAEQRKQDTAAAAAAPAAGDVAATPKEEVTTTPAEPITVATSMAGKPHELVAKPDE
                     GKIEFNSIRGYAVQKLKSVLSLVPEAKRAKLTAAISSAVEAAAVVVDPSLKPGAKPND
                     RPAVVKQLAAKLHELAGAIKAFGDTTNYPDSDPGEVARVDAEEKKAQEKMAAAGVTPA
                     APNASPNATPTAPASVSAPTATPAAPSAPNALNAPPTAATDAPKADAKPEAPKADAKP
                     EAPKTDAGNPTAPAKDAAPTAPNTMASSTAPAAPDAAKKDAEAPAAPVAAKDGNAPTG
                     DAKDPGKDPGKDPGKDPGKDPGKDPPPADPTTAKKTPTRPKPPETYETYSTWLFSNFG
                     DKITALKPPPQTDDQKAAIAKERAETQLAFAAERLVAVKAELTALKKEVLDRGAAMSK
                     KELEDNATMQAVVASEIEAVKENNGLTMPQLLAKAQDIVTKIKAATDGAKKEKAELER
                     VLTASSLLASQLDGPGFEKGRLLYVAIDTKEHPRLLDDGKDWELGNDAIAAMAVNYRN
                     WLRKYWRTEVMQDKTHAEIMFTRDEGKEDSRDGMTVEKVRDKVIDRLRGVDEAIKTKL
                     KNNQLDFPDAEQLMDKVHLLMIESAKKTNPAVNRAAGGSQVPVAPAAATSNGAPTPTT
                     STEAPPPAPVQQAKVDAPPPPPAKVDAPPAPRTDAPPADAGGKADAPKADAPKADAAK
                     DAPSADASKPGDASKTDAPPAAPPKVETPPPPAAKSEAPAAPNADPAKADAAKDPTAA
                     APNAPVAPPSTVAPTAPTTTPVVDPAAPKAADPNAPAPGTPAPEDKPQAPAHTRAESE
                     MELASVTNATVPQGKGEPGAKLKLSDPPEMIEWKSDSKDPIRKKDELISLTKKYLALD
                     KEKLPDTQKQRDQLLIKIQEKFAEMREAMAYARGEDSQLTNVRSQFEKFIGVKAMSNA
                     WPQGHEGLNRMATQAKLMILEQQTKVIESAKTVDVKLQALARQNPAWATAIGDKLGTP
                     VNIGFAGVVGKEFKDVLDALTTGSMVQKAMALINFSEFLAKEMMGPGAPAYMRRVSEN
                     IDNTQKEEAAEAAKNGFAEPKPFNLDKRKENAAPDKMGAKKGILTAPEFADELKAIKK
                     ADKGNDADPSKGVAAKLGVDDERQKSTIDENGKRVPNPEHKLDNPSQFSSENTLLGGD
                     MDSKAPQLPVELLDETQLDFLATQGEPPVKWTTEITGIKDPEERRKAKFKALKAEGIH
                     LSKAPGRVPTGKPEIPAPLQGGVWPAFGEGIPAKDSNGQAIEPKGAGGVDATSIVPDA
                     RVAVADQITVQDPKTGKDQTYIEGKAENLVDPLHKWIKTARENHAPLKAGISGTTARF
                     VGAARLLGATTNQAVVAMIGHLQAIEAHSFYEIVSGAGLGLAPGKYTPFAPNQQGMED
                     AGTEFVKNHVECGPDDADKKKKVILGQ"
     gene            25796..26590
                     /locus_tag="VMZ53_11435"
     CDS             25796..26590
                     /locus_tag="VMZ53_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29120.1"
                     /translation="MWTVAIALAAIVGAGCGRFGFGADDPGRDAPQVGDGGTDVVTMG
                     GDGGVDAPEFFACTGSEVCEGFESGFGVWMADAMVSLDATRAHRGNHSVHVHSPAFAA
                     GGSSYQAIYQSQTVSANINPFWVRGWFWLSARPNFDNAMELMTAERPSTSSGDYVFVH
                     ADSTNVYSQFDAQIMQTSTLVPVGQWFCLIWKVTRNTGTSGALELSGDVTVSLANTKT
                     DSTTSSLQIVVLGIGYASSNTPVAQPAQDLWIDDVIVSASAVTCSD"
     gene            complement(26591..27229)
                     /locus_tag="VMZ53_11440"
     CDS             complement(26591..27229)
                     /locus_tag="VMZ53_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HUS29121.1"
                     /translation="MSARPLVFATRNKGKLVELRELLPNIAVLSIDEAAQRIGAPIPE
                     VIEDADTFAGNAAKKAREVSAFTKLPALADDSGLEVDALSGAPGVISARYAGPDHDDA
                     ANNAKLLAELTGVAQDERTARFRASLALADVTGPLRDRVITADGTCEGRILTAPRGTG
                     GFGYDPLFFAPELGMTFAEAGIGPKSDLSHRARAMRAMKPQLLSYLQQLAEQ"
     gene            complement(27226..27930)
                     /gene="rph"
                     /locus_tag="VMZ53_11445"
     CDS             complement(27226..27930)
                     /gene="rph"
                     /locus_tag="VMZ53_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HUS29122.1"
                     /translation="MRPDGRRPDQLRPLEITTGFQKHAEGSALVKLGDTWVLCAASVE
                     KGVPPFLVGKNQGWLTAEYAMLPRSTHTRSKRDPGGRGKEIQRLIGRSLRAAVDLQHL
                     GERTINVDCDVLNADGGTRVSAITGAWVACALAIKKLVAAGQLADMRALRPPVAAVSV
                     GIVGGEVVLDLPYIEDSKADVDMNVVGDESGKLIEVQGTAEHDTFDRKQLDAMLDLAL
                     AGIKQLTVAQRQAAGV"
     gene            27994..29910
                     /locus_tag="VMZ53_11450"
     CDS             27994..29910
                     /locus_tag="VMZ53_11450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29123.1"
                     /translation="MPDDPDKPVDLGNAPTTPALEGTIAAPSPGPFAQGSGNPDSTLD
                     MPAAEAAALAENQIAHFRVIRRLGMGGMGVVFACEDTNLARQVAVKLVRDENPAYRAR
                     LLREAQAMARLEHPNVVRVYEVGQDRGRMFIAMELVDGETLTSWLRSFRTWQDTVEMF
                     MQVGAGLAAVHGAGLVHRDFKPDNVLVDRRGRARVADFGLARLDAQGGPSPMAQPMTK
                     SGVVMGTPGYMAPEQHFGADVDARADQFAFCIALREALGGRPVDERRWAQVPESVRSI
                     VNRGLSYDVADRFPSLDALLVALRSALAPVGVTDEVRAAHTSSRGRPSARARSRSMVD
                     DGIRKSEPRWIVLLLVLAIVGATAGIVAFVGTRKKPTPPARPPVATIAPADAAVAPPI
                     APSIDAAVAQVQEPLAPDAAVTMLPPTKTVVRTPRDAGIDAMQQQVVAAAPGQLPVNG
                     NGQGGAPAWGPYSAAWQNAKGGKTDVVRDAVKDVGYDSFDASWKKADLEKTIEGATGA
                     EKAIAQVKLGMVLRRAGDCKAASSQFQAAIKSLKPFDDESEGKWRGRAAVARSLCTLA
                     AGDPRLARAHLMDAINTFPGEIQLVHGISQFEDNADRALAYAHLITAQKQGDASVKAA
                     VKIYLGGYGLSLGP"
     gene            complement(29907..30701)
                     /locus_tag="VMZ53_11455"
     CDS             complement(29907..30701)
                     /locus_tag="VMZ53_11455"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS29124.1"
                     /translation="MARTVVLELAGQAALARDLAAGGVLVPDVLDMGEDVDLHLRGSG
                     DELRVSAKCVWVNGDKGSGLQLFGCDAEMKQKITSLAAAASATTSDSPTADTPGQPAI
                     DAPADPDETVDEDAEDADADAKKAPLNVHERLRGLTLAQQIKMAHNGEVAERIVLERM
                     YGKAVWEGLLRNPRLTGPEVARIARMGALPRPLMEIILGNGGWLQIPEVRRALLTNPR
                     LGTDQITKILRLLPKHELKMAAMQTIYPFAVRDVAKRLLKELGIGT"
     gene            complement(30868..32025)
                     /locus_tag="VMZ53_11460"
     CDS             complement(30868..32025)
                     /locus_tag="VMZ53_11460"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HUS29125.1"
                     /translation="MKLKGKVPVEQLDDERLTNIERKLVVAVSEMAPQRESSWSRRGL
                     ALAGVAMAMAVAGAVGWKLHAPASTPSRPTVAEAPFTLSTTDGTKVEVTHTGTRTDLA
                     MGAGTLSLDVKHDPNRLLVVHAGDTEIEDVGTKFSVDYDGKGHVDVRVTEGEVKVKRA
                     GKDFEVTANNAWTTEQGTITLAQLDSATTVVAQNTGANGTGDDIEIGNPKPIGETPKS
                     GSNATTNAGSAIGSNTNGSASGSGAGSANGKHKAGATNIKKAILAVELNPLSGTMTDY
                     IDQLKTATNDADKSRIMYSIAVQQHLQKSDGSAEHSIEGIINGRTKDEAYKDAMWLQV
                     RITCLRAVESPTDERAREKCRVSAHRYVNNIPTGVGAGIADQILKAIANGL"
     gene            complement(32022..32651)
                     /locus_tag="VMZ53_11465"
     CDS             complement(32022..32651)
                     /locus_tag="VMZ53_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS29126.1"
                     /translation="MAAAYVEDVLLVDRCLTGEPAATRELFRKHRGRVHASLYRVLGS
                     NRDMDDLLQEAFIQVFQSLRGWRAEASLSTWIDRVAVRCAYRYLSQKGRRIVTEEMGD
                     DDATPGGEGASPSRQMARDGVKRLYAVLDELGAASRLAFTLHEIDGRPMAEVAKLVGS
                     SVTATKLRVWRARKKVEEAAAADPILSEFVQPSAGSSGATVIELPKEGA"
     gene            complement(32701..33771)
                     /locus_tag="VMZ53_11470"
     CDS             complement(32701..33771)
                     /locus_tag="VMZ53_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUS29127.1"
                     /translation="MARRRRSAARGANAAELVALARDIASAPRTARRAKKTTSSSLEK
                     TTSSSLAKTTSSSLAKTTRSSLADIAAELDAAQRAVRDGRRATTNGSSLADVAAELED
                     AQRATRRAKRKPTSLADVARAIATPDARGAGDTSAMSCASALVEAAGGSPLDLALPAR
                     AISMELVSGRGHYERVLRAVLDAKVSVWIATANVKELMVEDHRARPGKRRSMKKRPYV
                     SVLAHLDELAARGVELRLLHAELPSGPFRTELAQHPKLAGGGALALRRCPRVHLKAVI
                     VDGELLYLGSANWTGAGLGAKGSGRRNFELGFVTDDAQLLDQVQGLYERIWAGGECDA
                     CKLHEECPGPLVQLKTRAKTKG"
     gene            complement(33771..33917)
                     /locus_tag="VMZ53_11475"
     CDS             complement(33771..33917)
                     /locus_tag="VMZ53_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS29128.1"
                     /translation="MPAGRFGTIDEIAAAAVYLRSPAAAYVTGHIMVVDGGHCVSTPS
                     LMGA"
BASE COUNT         4995 a        11026 c        11114 g         6786 t
ORIGIN      
        1 tgcattcgca ggcgcagcac ccgcaggcgg tgcagcgttc gcagccggag ctgccgcgac
       61 gggcgtcggc gccggaacca cgggcgcgtt accttcggga accgtcgcga agtgagcgtt
      121 ggccgcggcg atcgcgcccg ggcgatcacc gacgtagagg cagcccttgt aaccaccggt
      181 cgacttcgtc tcgtcgagcg tgcggcgatc cttcttcgac tttgcggctg ctgcgtagtg
      241 ggtcgtgacg ctctcgagcg agatgccggc ctgcttgagg ccgtgctcga gagcgacgga
      301 ctcgacggct tcccagatgt tgccggcagg cacgcctgcg ccaacacata ccttgacctt
      361 cttgcccttg acgatctccg gcacgaccgc gttggcgtac gcgttcgaga gcgcgcccca
      421 catctcggga tcccagccgg ccttcttgcc gtcgagcgtc ggcatgccgt ccatgagccc
      481 gccgatcgcg gacgtctcga gcgtcaagct gccgctcatc gactcggcga ggctgcggcc
      541 ttcgtccttc gaccagaagc cgaacgacgt ggcctgaccg aacgccgact tgcccgcctt
      601 cgcgagctcg tcaccgatct tgtcgaactc tttgagcgcg ttcttgaaga ctggcgactt
      661 catgtcgagc cagcccttcg cgaagtcaga gtacgcggcc ggatcggtca tcgccggctg
      721 cgccttcttc accgcctcgc agatcttgat ccagatgtct tccgtgaagg gcgcaacgtt
      781 gacgttgagc ttgccctgca tcgacatcag ggcgacgcgt cccttgaacg tgttgtagta
      841 cgggtggagc aggggcttgt cgatcgagcc gctcgtgacg gccggatcct gcgcgacctt
      901 gagccacgac cacgtgatct tcagcgggtt cgacagcgtc tgctgatcgc tcgcggccgc
      961 gctcttcgcg ctctcgtcgc ctgcgaaggc tttcttcgcc gtggtgtcga gcgtcgtcgc
     1021 cgcggtcttc gtggtctgaa cggtgtcgta cgcgggcgtg cgcagcgaat cgggcgctgc
     1081 cttgatcgcg ctctcggcct cgccgatctt cgtgctgacg ggcgtcggcg tgctcgccac
     1141 catcgcgaca ccggtgagct cgtcgatgta gagcgtgtgg ctctcgccga ggatgtcgag
     1201 cgagactttg acgccgatcg ggcccttggc cttcttgtcc ttgtctttct cgtcgccggg
     1261 cttcggcgca gctgcagccg cagccgcctt cgcatcggcg gccttcttct cttcttcctt
     1321 cttcttcttt gcttcagccg ccgccgcgtc gagcttcttc gtctcggctg cggtctgcgc
     1381 gttcttttga tcgacttgtt tgtcgtactt ctgcttctca ccgtcgagcg cagccttctg
     1441 ctgctgctgg ccggccttgc cctcgtcgat gaccttctgc acgctcggat cgaccgcgcc
     1501 cgcgcccggc ttggcagcac cggccttcgc cgcatcacca ccgggagcgc cctcgggacc
     1561 accgggcttc gcgccgtcac cgggcttcgc cttgtcggga cccttcttgt cgccgaacag
     1621 cgccgagacc atgctcgccg tcggcatcgc cacgacaccc gcgggcttga agctcggatc
     1681 gtactgcttg tgcttgccgt cgatggtctt gttctgcgag atgatgccgt cgggaccggc
     1741 agcgaccttc ggacccggtg gcggcgtcgc ggttgtgctg ccgccgacct tcggcgcgcc
     1801 accctgattg ccttcgcctt gcggcatcgg tgagccacca gcgcctgcgg attgtgcggc
     1861 ggtattcgac gtcgcaccgg gcgtcgggtt cggtgcgcca ccttgcggcg catccttctt
     1921 gcccgggctc tgcacctcgg ccttcttctt ctgcggcgga atcacgcctg cagccttcac
     1981 actcacgccc ggatcggcgg gcagcgcggg aagcgtgacg gcgggcagaa cctcagccgg
     2041 aggcggcagt gggatcggag gcggaaccgg aatcggtgcc ggcttgctga gcggcttcgg
     2101 catcggcggt ggcgcgggct tcgccggcgg tggcgccttc tcctgcgcga ccggtttgac
     2161 ctgcgcgcct tcttgcgcgg ccgccttgcc ggcttcggct ttcttctcct gcgccttgtc
     2221 ccactcgcct tggatcttgt tgacgttctg atcgaacgcg gtcttctgct cgatgccgac
     2281 ctgcgcgtgc gcgacctctg ccttgccgaa gtcagcagcc tgcttggcga cctgcgcggc
     2341 gtggtcctcg tcctgcttct tctgtttctc ctgcgcctct ttgagatcct tttgatactt
     2401 ctcttcgatc agctgctgga cgatgacgcg ttcttcgatg acgttgacgc cggtatccgg
     2461 gttgatgccc ttcgccgcga tctgcgcgcg ccgccagcct aggacgaggc gacctgcacc
     2521 ggtcggatcc ttcttcgcgg tcaccgcgac tgccttcgcg agacgctctg tcggcgtgct
     2581 catccgcagc tcgggaacca tgtcttcggc ctggtcggcg tgctcctcgt agaacttcca
     2641 gtgcttgtcc tgtacgggga acatcgacga gaagtcgacg ggcgccgcga ccatgccctt
     2701 ctcccagcct tcggggagct cctggaactg cggcgcagcg ttcttgaacg cctgcagcac
     2761 cggcgtgatc ttttcgcgcg cctcgcggtt cgactcgttg tcggctgcct tctggccgcc
     2821 ttccttgacg cccttctcga cgttgtcgaa cgcgtcttcg cggagcgaga ggccgcgcgt
     2881 gagcttcgtt ccgccgggac caccgaactg cagaccgacg ggctggaacg gctggaaccg
     2941 gccgaggtcg atgtgcgcga gggttgcctg cgcgagcgtc cacttcttgt cgacgatcgt
     3001 ccaccagccg atcgacgcgt acagcgtggc gatcagcgat gcgacgagat cgagtgatgc
     3061 ggcgacatac ggatcgatcg tgatcagcgc gccctgtccc gattggtagc gaccgtcgac
     3121 gtcggcgccg agctcgaggt tcaggcgcgc catgatctcg gcgccgatac ccgcgctcgc
     3181 tgtcgcgatc aacagctgga tctcgccgac gatctgaacc gacagcgcca tgtacacgta
     3241 ggcgcccatc gcgatcgagc cgccgaacgt gaacgacggc atgccgccct tgtcgagggc
     3301 gtcgaggccg ccttcgagct tcgggttctt gaacttgatc ttcggcatgc ggtagccggc
     3361 gccgacctcg atgccgaact tgagggcgag ctgcgcgaca ccgacggtga ggagcggaac
     3421 ggagatcgac ttgccgaact tgccgacggt gtgctcgtcg ttgttcgcgt ccttgtcatc
     3481 cggctccatg ccgatgacct gcaagccgcc ttcgctgctg tagcgcaggc ccacgtcgag
     3541 cgtgagcgtc tcggtcggcg tgagcttgat gcggccttcg atgaagaagc ccgagccctt
     3601 gatgtagccg agctcgacga cggcttcctt tacgagcgag tcgtccttcg ggcggagctt
     3661 gacgccgacc ttggcgctgg tctgatcgag atcgccgtag accgtcgtcg cctggaaacc
     3721 aggaacggtg agcgggaagt cgatgtgcgc gaagagaccc ttcgacttcg agtagcccgc
     3781 ctcgaagtta cccatctcgt tgttcttggc catgggcgcc ttgaactggg tcttgagggt
     3841 cgccgacagg tctccatcgg ccacgtacga gataacgatc ttcggatcga cgagcggcag
     3901 gaacgaaggc gggacgaggt tgaggatgcc ctccgccttc atcaccttcc attttttgat
     3961 gtcgacgttg atctcgcccg actggacgaa ctcggcgatc gcggacgtct tcgcaggcgt
     4021 gacggtgagc ttgacggcgg tgtcgagacc gcccatctcg cccggcgtga ccttcgcgtt
     4081 gccctcgatc ttgagggtcg ggaagtccgc catcatgtgg aggttgccct ccttgaactt
     4141 gttcttctcg acgacgatcg agccgtcggc gatgaccggg ccgagctccg cgcgggtgac
     4201 cgcgagcgtg ccgccgaagc cttcgccgct cttgtagttc gcgtcgagcg aggccgcgac
     4261 cttgaagtcc gcgatcgacg tgatgcctgc ttttaggccg aagtcgagag ctggcggtgc
     4321 gccgccgccg atgtcgacac cgaacgtggc aacgacgctc gagaacttca cgcccggaac
     4381 tttttccgac agcttcgtcg cgtcgatctt gccgtcgagg tggaacgaac ccgcgaggcc
     4441 accgccggcg ttcgcgcgca ccgtcatctc cgcttcgcag agattcgcga tgtccgcggt
     4501 ggcgtgaccg ctgacgatca gttgcttcgt gttcgaggtc cagttcgccg tgatgtggct
     4561 ctctggcttg aacgtcaccg ggccgagcgt cttcggcttg agcgtgccgt tgatcgtgaa
     4621 gaccttgttc gcgtagtgca gtgtcaggtc cgagccctca gcgagctgcg gaatgacctt
     4681 gtgcatcgcg atgccggcat cgatcgtgac gcctccgccc tgcgaccagt gaatctgcgc
     4741 gcccttctcc ttgaaggcgt cggacacgct gccgaggttc ctgatctcct tgacctggcc
     4801 tttgacgtcg aacttgttgt tcgtgacctc gaccgtcgcg ctcgcaacga attgatcggt
     4861 gtagttcggg gcgaggctga cctcgatcgg cttgttcgtc gagacctggc ggttttcatc
     4921 gacgcgcacg tcgatgtcga ccttgtcgaa gaccgcgaac gggtcgacgg tcaccttggc
     4981 gttgcccgtg aacttgcggg tcttcgtctc gtagtggaga tggaagtcgc ctttgccgac
     5041 gttcggagcg acgacggagg cttcgagatc agcgacgagg ccactggcct tggtgatgct
     5101 gacctggctg ttcggtccga gcgtcgcggt cgcgaagctg ccgagtgcga tcggcggcgt
     5161 gttcgcgcgg agcttcgcga caaacgagtt gttcgtcgcg gtgacgttga actgaacgcc
     5221 gtcgagcgct ttctgcttcg gatcgacgtc gatgtcggcc gcgaactcct tcgtcttgat
     5281 gttgaaggac gcgctgttga tcttgccctc ggcaacaccg gggatcttga acgtcgagtt
     5341 cttgccgaga tcgacctggc cgctgttgag gtcgaccttg ccgcccttcg attcgaagaa
     5401 gtcacccttc ttgacggtga tgtcgacgat ctcgccgtgc agatccgtcg tgccttctgg
     5461 aatctcgagg cgcagcgtgc cggacacttc tggatcgccc gacttcggat acttcgccga
     5521 aatctggacc atgccgccga agctgagctt gccgttgtcg taggtgatct tcgcgccggc
     5581 gccggcgctc tcgatctgga cgtcggggac gttcttgacg gccttgttga tcgccttctc
     5641 ggcctggatc gaggcgacgt atttgttcgt ctgcttgttg ccctcgagat gcgtgatctc
     5701 gatgtccttc agcttctcgt tgccgaaggt gatcgggcca ttcgagacga agttgccggt
     5761 accgtccgag cctgcggcgg cgtggaccga accttcgagc atgtggttcg tgatcccacc
     5821 gagctcgaag ttgccttcgg cggcccagtc gaaatggccg tccttccatc cgatcgtggc
     5881 cttgacctcg gagccgcctg acttgcccgt ggcgtcgagg tgaccgtcga gcttcttgtc
     5941 ggccagcgtg atgtggccgc tgttgatcgt gaccgacgag ccttcgctga cgacgatctc
     6001 ggtgcccgac gccgcggtca ggttcgcggt gactttgccg ttttcatagg tgccggtgac
     6061 gctgctgaag atcgtcttgt ccttcagcgg accgacctgg acgccgacgt tcgttgcact
     6121 cagcgcgaac gcgttgcttc cgctcgagcg cttgtatttg acgttgagct caccgcgaag
     6181 atccttctgg atcttcttca tgtccatctt cgcgtcgatg gtcagatcga tcggcttgcc
     6241 cttggcagcg atcaggtgtg catcgagatc gccgagcgcg ggaatcttcg ccgtggccga
     6301 gatatcgccg gcgatgtcga gcgcggtcgg cgtcttcgaa acagtgagct tcgagccctc
     6361 gttgatggtg accttcgccg ggccgacgtt gacggtggcg ccaccctcga cgctgacctc
     6421 gccggtgaag ccgccttcat cggcgtaacc gatcttcgcc ttggccttga agccacctgc
     6481 gttgttgagc gcgaagccac ccgtgatgtg gccttcgacc ttcttgccgt cgatcttgat
     6541 cgatgagtct tcgagcgaga tcgttgctcc acccacgtca acgctgatcg cttcgttgag
     6601 gtgaagctcg gctttgactt tgctctcacc accgagcgcg acctcggaga agttaatcgg
     6661 agcgagcttg ggccaggtga agtggaggtc cttgctgtcg agcgacagct tgccgccctc
     6721 gtacttgatg ttcgcgctgc ccttgaggcc ggggagattg aacggcactg tgccgcccgc
     6781 ggtcatctcg agcttctcgc cgttgaagaa gatcgtggcc ttcgcctcgg cgagaccggc
     6841 gaccttgatc tcgccgttga cgaaggcgtt gaacgatacg ccttctttgt tcgccttcag
     6901 gtcgaggtcg agcttgcctg cgccatcagc gaagccgttc ttgatctcga cttcggtgtg
     6961 cccgatgacc ttgagctcga gctcgccagc tttgctcgag atcgtgactt cgaagtcggt
     7021 ctccttgacc gcgaaatgct tgctcgcctt gaagtccggc ttcacgcgga ccgtcgtgct
     7081 gatctcgcca gcgccgactt cgacgctgac ctccttgatc gagagcatgt tcggcgcgtt
     7141 gacggggatc gtcagcttgc cgctcacgcc ttcgttggcg tcgacggtga ggtgaccttc
     7201 cgtgccgctc agcgcaccgg aatcgatctt cgcgaacagc gtgccttgct cgaccttgtc
     7261 gcctgcttcg tttggcttga actgcgcctt cgtgagcttg agtggcccga ggcggttcaa
     7321 cggcgccggg atcgtgaatt ccttcttgcc ctcgccggcg tcgtacgcgt cgacaaaacg
     7381 cgagccgaga tcgagcgcct gaccgacgag cctcggcgac cacttcgtga tcttcttgtc
     7441 gctgtcgccc tcggtcttgc gcgaaatcgt gccggccgcc gcgctttcgc gaacgcggaa
     7501 tgcaccggct cctgctaccg ggccaccggc gaacgcgttc gcaatgcgat cggcttcgcg
     7561 ctcgacggcg ctaccaggct ccgacatcga gccgggcgcc gcgttgccgc ccgcgcgctg
     7621 ctgcgcgacg tggacgacct cgtgcgcgat gagtcgcttg ccttcggcgc tcgacggatc
     7681 atacgcaccg tcggcgaagt agacgttctt accgcgcgcg aatgcgacgg cgccgtggct
     7741 gctcgcttcg cgggccgcct tcgagtcgtc gtgaacgcga acgtcgctga agtcgatgcc
     7801 gagcttgctc tcgagctctg ctcgcaggtt cgtcggcagc ggcttgccgg cgcggaaatc
     7861 gcggtcgtgc tcggcgatcg agatcagcgc gtccgcaatg cggaggatct ggtcgaggcc
     7921 gccattgtcg acgcccgcga tcgtcgagcc aacctgaccg tttcggaact tccgcagcgc
     7981 atcgcggatg ctgtcgccgt cggagtgggt gatggtctcg gcaatgacct cgcccttcgt
     8041 gaccatcttc tcgaacgcct gacggaggcg ggtcgccggg tcgagcgccg acgcatcgat
     8101 ctcggacttc ggcccaggga cgtcgtggct ctgctggatg tcgccgcgct tggcatcgcc
     8161 gttctggaga tcgagaacgc gcgtccactt gccgggatcg agcagtccgg catcgccgtc
     8221 gagaccacgt gcgagctggc cgagctcctg cgcttcgtcg acgaggcggc ggaacgttcg
     8281 ctccgtccgg atcggatcct tgccggactc tgcctcggcg cgtgcccaga gtgcgcgcgc
     8341 aagctcttcc gaaatgccag tgctacgcgc aagagcgcgc cacacgacct catcatcacg
     8401 gcgattgaga agctctaagc tcatcccagt cgaagaccca cgtcagtatc caagtttacg
     8461 tcggtcgacg ggtcgttctc ccccggtatt gcccacaatt acgatcggtt cagtcctccg
     8521 atcggccgat cgcgggtttc tccgagcgga ctcccgttgc tgagatggga agaagtcgcg
     8581 gagggcattc gtagccggtg ggtaggcaga tgtgggccgc aggctgcgtg atatggtcgc
     8641 gcgcgtgagc cgcgacgagg aggagctcga gcgggcgaag gagcatctgc gttcgttgca
     8701 gatgtcgctt cccgaattaa tcgaatatct gcggaagctt gcggagattt acagccgccc
     8761 ggatatcgcc cgcgccctgc cgaacgcgca gcaagaggca gagcggatcg cgggcttgct
     8821 cgcctacatc gagcgctaca cgcgcgtcga cgcgcagacg ttcggcccgg tggacctccg
     8881 cgcggtgctc gaagaagcag tcgcgctgac gcgcgcggag atcgagcaaa gaggccgggt
     8941 cagcgtcgcg tatctcgaag cgccgctcgt gcgcggcaat ccgcgccagc tcggtcacgt
     9001 gttcatcgcg ctgctcatca acgctgcaca gtcgctggcc gagggcgcgc cgcacgccaa
     9061 ctacgtcgcg gtcgagctcg atacgaaccc ggatggtggg tgggcacgca tcgcgatcgc
     9121 cgacagcggc tcgggtatct atccggaaga cgtcgcacac atcttcgagc cgctctacac
     9181 gacgaagcgc ggcgccggca tgggaatcgg cctagcggcg gttcgcgaga tcatcacggg
     9241 actcggcggt cgaatttctg tcgagagcgt gccgggcagc ggcagcttgt ttatcgtcga
     9301 gcttcccccc gcgtcatgaa caaggtctcc gagcgagcag gttccctggg actctttgcg
     9361 accgcgaatg cgttcgcaga cgccaagctc gatcccgatg tcgcgccgcg cgacccgcgc
     9421 ctgaaggaag ccgcagcgac ggatctcaag cgcctgttcg gtcagatggc gcgtcgcggt
     9481 cacgacctcg agctcgtgcg cgccggctgg aagatgctcg aacgtcgacc gctcgagcgc
     9541 gcgctgtttg ccgtggacgt gctcgcgcag agcatcctcc cgcagaacga gccaatcctc
     9601 ctcgaaatgc gaacggtcgc gaacatgctc ggaggagcgc gcccgctcgg cgtcgaggtc
     9661 ggcatcgatc tcaacaaggt gtcggcgagc catctgacgg agcaacacgc gcggcgtccg
     9721 gagatcgccg cgagcgacac cgtcgatctt ctcccgctcg cgttcgagca ccatttgggt
     9781 gcgctcgccg agctgcatca gcgtggcgac gccctcatct cgcaagccgg cacctgcgag
     9841 agcatccaag cgttcgcgcg cctcgcagct ctcgcgcgtc tgccgacgct cgcgtccgtc
     9901 tatctcgact ggctcgtccg cggcgtgggt tggcgcgttc cggttctgga tctctgcgac
     9961 acgctgctcg atgcaggagt gccgcagaag attccgggcg atgggatcca ggcgggcgac
    10021 attcccgaga aggagatgcg ggactccgtc gagtatctcg tctatcgcac gcatctcagc
    10081 ctcggtgatt acgacagggc aaacgcgctg ctctcgcaga acatggaaca acgcccgcgc
    10141 tggatcggca cgccctcgta tcgcctcgat gcggttcgcg gtcatctagg cgcgttgttc
    10201 ggccacagca acgacgtcac gctggcgcgc atcgaagctg tctgcatgga ggatcgcctc
    10261 tggcgctacg gcgcgaaggt tcgcgcgatc gtcgcagctg cgcgccagcc aggtcgtgcg
    10321 agcgagatgt tccacgcgta cgtcggcggc ttcggcaacg actacgacat gacgttcagc
    10381 gtgatctcgc tcgcgcccga gcaagtgaaa cgtgacgtcg cgcggatcct cgcgcgcgag
    10441 gcgtactact tgccgcacga gccggccgtc tggaagctcc tcgctgcgct cgcaggcaac
    10501 gacaagcccg tcgatgaagt cgacgctcgc ttgaaggcac aggcgcgcta gtggcccgcg
    10561 tggacgccca catcgacgtc gcgctcgtcg gtggcggcat catgagcgca acgctcgcga
    10621 cgctgctccg caagctcgac ccgacgctgc gcatggtcgc gtacgagcgc cttgatcacg
    10681 cggccgccga aagttccgac gcgtggaaca atgcgggtac cgggcactcc ggcttctgcg
    10741 agctcaacta cacgccggcc aagcccgacg gcaccgtcga gtgtgcaaag gcgatcaaga
    10801 tcgcagggca gttccagcag agcgtggagc tctggcgctc gctcattgcc gcgggcgagc
    10861 tgcccgtcga gtcgacgttc ctgcgcgagg tgccgcacat ctcgttcgtg accggcgacg
    10921 acgtccccta cctacgcacg cgcgcgaaga cactccgcgc ggagtgcccg ctgttccacg
    10981 atctcgaata cacggaagac gtcgccaaga ttgccgagtg gatcccgctc gtgatggaag
    11041 gtcgtgctgc gggcacacct gtcgctgcga cgcgcatgat gcacggcacc gacgtcaact
    11101 tcggcgcgct cacgcgatcg ctgatcaact ggatcgacga cgaatcgggc ttcgcgatcg
    11161 agctctcgcg cgaggtccgc gacatccgcc gcgcgcatcc cggcgaaggc tggctcatcg
    11221 acatcaagaa cctcgcaaca ggagccgagc acacggtcca cgcgaagttc gtcttcatcg
    11281 gcgccggcgg ctactcgctc aagctcctcg agaaaacggg catccccgaa gcgagcggtt
    11341 acggcgcatt cccggtcagc ggccaatggc tgcgctgcac gaaccgcaac gtgatcgatc
    11401 gccaccacgc gaaggtctac ggcatggcgg aagtcggcgc gccgccgatg tccgtaccgc
    11461 atctcgacac gcgttggatc gacggcacgc gcgagctgct gttcggcccc tacgcgggct
    11521 tcacgacgaa gtttctcaaa gaaggctcgt ggctcgatct gctcggctcg atcggcatcc
    11581 acaacatgcg cccggtcatg gctgccggct tcgacaatat gaacctgacg aagtacctcg
    11641 tcggccaggc gctactctcc gaggaagaac gcgtagacct cctacgtcgc tactgcccaa
    11701 cggcgcgcgg cgatgactgg gagctgcaga tcgccgggct tcgcgtgcag atcatcaaga
    11761 gcgatggtca cggcggcggc gagctgaagt tcggtaccga agtggtcacg agcgccgacg
    11821 gcagcatcgc agcgctcctc ggtgcctcgc ctggtgcatc gacagcggtg tcgatcatgc
    11881 tcgagctctt gcagcgctgc ttcccgggga aatgggcgtc ggctgaatgg cggagcaggc
    11941 tgagcgcgtg gattccgtcc cttggttaag ataaccgcat gagctttgcc gagaccgcaa
    12001 accgacgctt cctgcgctcc gtgtcgggcc acctcggcga gctgtcgtgg gagatcgacg
    12061 cggacggcta tcagcgtttg caggtccctc ccaccgcgga tctcgacatt ctgctgcggc
    12121 ttctcactcg ccgtggtctc gaccccgcgc tgacgacgct cctgctcgat cgctttgcga
    12181 accggcccct cgagcgggcg ttgatcgcat gggatgtagc cgccggatcg ttgctcggaa
    12241 gcgatgacgg tgcgctgctc gaacgcgttc gcgcagagct cgctccgctc aagaagatcg
    12301 gtacgtccgc gtcggtggcg ctgtcgcgcg gtcaggtcgg cgcgctcatc gaggcggtag
    12361 ttgccgtggg ggaaaagccg atgtccgaag gcagcgcgcc gcagccgagc ctggctgacg
    12421 ctgcgacttc aatcgccacg gacactgcag gtatcgaggc ggcgctcgcg ttcgctgatc
    12481 gactcgcgct ggcgcacctt ccttcgctct cgctggcatt tgcgcagatc ctgtattcgc
    12541 gacattcgct tccggctgca ctcgatcgga tcgtcgagac cgcgctcgat tacgaacggt
    12601 tcgattcgat ccctgccatg accgagtcgg acgatcgctc gctgcagcgc cagacctact
    12661 tcggcatgcg agtcgcgctc gcccagctcg atgcgcagag cgctgcgaag atgctcgcgg
    12721 agatggccgc gaagcaccct gccgtcgccg atctgtcgca tccgacactg gccgctgtgc
    12781 gcgtcgagct cgaagtcgtt gctgataggc cggtcgacta ttcgatggtt gcaacgctcg
    12841 aggcactcgc gcctgacgga tcgaaatggc gttacggctc gcgcgtgcga gacgagctgc
    12901 gcatcgaggt cgctccgcaa aattcggcgc tgacgctcga cggattcatc agctcgttcg
    12961 gtaacgacct gcgtatgtgg gcgcaggccg gctatcacga cgaggttcgc actgagctgc
    13021 tcgccctcgc ttcgcgcgag atccgatatc agtcgcacga tccggatgcg tggcgctcgc
    13081 tcgcggtatt tgtcgaggat ggctcgccga tcgagctcga gctccaggag cgtttggcgg
    13141 cgcagctaga cgccgtcctc gtagccgtca actgaagcat gacgacgaac gtcgacttca
    13201 cgaagtgcga tcagcccgac gagcttctgc agctcgcatg gaatgcaggt ctcgatcgca
    13261 ggacgatcat ccgcgagggc agcgacgccg cgcggttact gcttgccggc gagaagaacg
    13321 agatcatcac gctgttctgg cccgtgccgc gagccattga agccgtcgat cgctgggccg
    13381 gcgatccgcg tgactccgcg ggcaacgccg aaacgatgcg ccacttcgcg agcgcagtcg
    13441 taccgggctg cgtgctgggc gccgcggtcg cgcactggtt cgtatccccg cacctctcgg
    13501 agaacaacag cgccaccgcg atgttcggca tcatcttcgc gaccatcgtc gtactcgggg
    13561 tcgtgttcaa agtgctgatc ggtgcatcga tccggcgtca ggtcgcgcgg ctcgacgagt
    13621 cgaaagcact cgaaatcgtc ctcgcccggc tccggcgtgg catggtcgcg aaaccccggc
    13681 tgatccccgt cgtcgtcaag gacatccgcc gaggcttcgc ggcaagcgcg agcgatcagg
    13741 cgaactgatc acggcagtcc gagcgcgtgc cggcactcgt cggctgtcgg ttgcgtcgcc
    13801 ggcagcggca cgccgacctt gtcgaggatc acgcgcaacg tctcgtcggg gatcgcgagc
    13861 gcattcggca acggcttgag gatttgctcg cggttcgcct cgctccatcc cgcgagcctg
    13921 cgcaggaatc cgagtagatg gtcgcgacga tagatcgcca tcgctctcgc agcggccgat
    13981 gcgcagctgc gatcggaagc gtcttctgtc cagcgactcg cgctctggat cacggcgttt
    14041 ggctcgaact gcgcgaccac cgtttcggcg ccgtcgcgcg gtggctcgcc gttcaatagt
    14101 ccaacgatca gctttgcgac gagcggaccc tgtaacggta ggtccaccgc cttgcttgcg
    14161 agcgcgccgc acagctccgt cttcaacgtc gaatcctgat agagctccgc gtgatttgtt
    14221 gcgatgtcgc gtagccgccc gagcgcgaca gcagatccgg gcttccaagc gaagacgatc
    14281 tcgagcgctc gacgtctcac cgtcggatca ccatcggtaa gcaacgcgct gacgacgggc
    14341 cacacatgat cgaatacaaa tgccggccgc catcgcatcg cgaagtgcca cggatagtcg
    14401 tcgccgaacg cgggtggccc atcggccgat tggcgaagca tcttctcgag ctcggtgtcc
    14461 gacgcgacgg gcggcgaggg cagggcgtcg tcagtagctg cacccggatg cccgccgcag
    14521 caccatcccc tgtgcgcgtc gcgcgccgcc cgacgtttcg acgcgtcgac acaggtgcag
    14581 cggcacacgt acgtccgcga gccgagtgct gcagtgacgc gtgcgctgcg ctctgcccgc
    14641 ggatcgatga acagttgctc gatttcgtct ccgataggcg ttggctgact cagcgcacac
    14701 gcatcgaggc tacggatcca cgagcggcac gactggcaga acagccgatt gcacgcgacg
    14761 agctcctgat cgctcacgaa gcgagcgctc ggcacctcgg gaaaccaaat gtccatgccg
    14821 gcgagcattc cgccatcggt acatgacgga tccggtgagc tgggcgcggc catgccggga
    14881 ctatagctcc gtgtcgctca cgacgtcgtt gcgccgaacc actcgcgaaa gtctgcgatg
    14941 cattggtctt tacgcgcgcc gctgatgaag atcgccgcag acttgccgcg caggttctgt
    15001 tcggtcgtga tgaagtccca ccgcacagca aggctcgcgt tgtttgccaa cccgatcgcg
    15061 gtcacggtca tgctcgtgtc gtgcggacct agcggcgtga ggacacgcat gtaccggacg
    15121 atccggacgc cgcggccatc cgagtcgtac tcctcgactt gcgtgaagta gcactcgata
    15181 ccctcgtcgt tcgtgtattg ccccttctgc ccgagtgcac ggtctcctga ttgcggaaac
    15241 gtcggatcga tccagcgcgc actgcttcgc acaaacgcca cgagctcggg ttcgacttca
    15301 tcggccgagc tcacgattcg caccgctcgc gggcccttgg cgatttcgca ggtctcggtg
    15361 tcgtcgctca tggtggaggc aatcctacgt ctggacgagc atcgtttcga gcggacgcac
    15421 ccatgagtcg tcgacccaac cgaagccgcg ccgactcctc atccatgcgc ggaattcagc
    15481 tggcgtggtg agctttgcct gaatgacgcg cgcgccggcg atcacgatga gatgcccttg
    15541 agcaggctta tgcattcgat ccgttagcag cgtcatcgcc tgtcgccagc gacccagaag
    15601 tcgattctct ccatcgacga ggagtccccc gagcgcgcaa acacgatcgg cggtcatttc
    15661 atctcagtgt cgtcgctcat ggtgaagaca atcgtaacgc gtacaatcag gccatgacgt
    15721 tcgaagaggc gctcgcgtca ccgggacgga aggttcagat tccgctggcg aaagtaaggg
    15781 acggcaagcg tgcactgttg atcctcagcg tcggcacgga tggcgcgcag cagctcgcgc
    15841 acagtgacgt tccggaagct gagatcgacg cacgggttgc agacttgaaa gcgcgcggaa
    15901 tcgatgtcgc ggcgaccgat attgattccg acgccacgtt tgtgtggatt cgcaaagacg
    15961 gtggcatcga ggtgtacgac tcgagctgca aggtattcga ggctgcaggc gagagcgcga
    16021 cgctcctcga cggcacgtcg atcgcgcgtg ccgatatcgc gcgcgtcatc gcgtacgcag
    16081 acggctacgt ccatcgcggt gtgaaggcca cgctcaaatc aggcactgac gtcaatctcg
    16141 tcgcccaggt ctcgacgtct tccatctcgg atccgatgta cacgcgcaac gagctgctgt
    16201 tcgagacggg atggaccgcg acgatcggaa tcgcgatcgc cgactgggcg ggcgccgaat
    16261 tcgacgatca ggtctagttc ttctcgtcga gcatcgtctc gagcggaagc acccatgcgt
    16321 cgtcgaccca accgtaggcg cgtcgacttt cgatccacgc gcgaaattcc gcgggcgtgg
    16381 tgagcccggc ctgaatgatt cgtgcgcctg cgatcacgac gaggtggcct tgggctggct
    16441 tgccgatcca atccgtcagg agcgtcatca cctggcgcca tcgaccggga cacgcggcat
    16501 cgagctccac gatcttgtcg gcgaggaagg tgcgcgtcgg ctcgtcgtcg aagagtcccg
    16561 gagaatgtgc gattgcgttg ccgacgacgt cgatacgctc ttgggttctc gcggccgaga
    16621 agtccttcaa gagccagtcg gtcgtcttcg cgtcgtaacc gagctttgcg gatcgctgcc
    16681 gttcgaagtc cacggttccc gcgggtccca gatcgtcgtg aggcagcgac gcgcgcggga
    16741 tgaacatcag ccgcttgatc agcgtgtcga cacgggctgc gagctcggct cgctctgtaa
    16801 cggcgtgcgc gatgagcact ccccatagcg tgggcgcgct gcggtactcg ggaatcggat
    16861 acccgagcgc gacgcgaaga gggtttgctt ctgcgtactc gacgagcttc acgattccga
    16921 ccgcacgagg aaacctggtg aagaagaaca ggacgcgtcc gacgacctgc ggatcggaat
    16981 cctgcagccg gtccgcaatg gcggcagaaa gcgcgtccgc gacggacagt ccctgcagat
    17041 agccgtagag ccacgacagc cagatggcag gttcgggacc gatcgcgagc attcgatcga
    17101 gctcgcgagg acagctcccc ttcaagacct tgtcgacgag ctgtgcccag ttcgtcgccg
    17161 cgtcgatcct gagaccgttc agtgtgagcg gcaggtcagg tgctgggtga cccgcacatc
    17221 tccacgggac gttcggatcg gaggcaagct cgtgatcgtt gtcgatcggg tcgacgctct
    17281 cggcctccca atacgtgcat gtgcatgcat acaaccgcag ttgtctcgat agcgcgtagc
    17341 gcgattcgat gaacgggaga acggaccaat cgggcgctgc atgcagcgcg cgtaggtcat
    17401 gtggtgcgtc cttcttgaag ccgagcttcg gtgggccggc gcgaacccac tccttgcagt
    17461 tggaacagcg caggcgcccg cagccgacgg gaaggtcgag acccgtctga tagtactcgc
    17521 acggatccgt cagaagtcgg gtgtctccgt cgacaaggag acctccctgc gcgcaaacgc
    17581 ggtcggtcat tactgaccga ggataacctt cttcttcttg tccgcgtcgt caggtccaca
    17641 ttcgacgtgg ttcttgacga actcggtgcc cgcgtcttcc atgccctgct ggttcggagc
    17701 gaacggggtg tacttgcctg gtgccaaacc aaggcccgcg ccggagacga tctcgtagaa
    17761 gctgtgggct tcgatcgcct gcaggtgacc gatcatcgcg acgaccgcct gattcgtggt
    17821 cgctccgagc agccgtgcgg cgccgacaaa gcgcgcggtc gttcccgaga tgcctgcctt
    17881 gagcggtgcg tgattttcac gagctgtctt gatccacttg tgcagcgggt cgacgaggtt
    17941 ctcggccttg ccttcgatgt acgtctggtc cttgccggtc ttcgggtcct ggaccgtgat
    18001 ctggtccgcg accgcaacac gcgcgtcggg aacgatcgac gtggcgtcca ctccgccagc
    18061 gcccttgggc tcgatggctt ggccattcga atccttcgcc ggaattcctt caccgaaggc
    18121 cggccatacg ccgccttgta ggggcgcggg gatctcgggc ttgcccgtcg gaactctgcc
    18181 gggcgccttc gacaggtgga ttccttccgc ctttagagcc ttgaactttg cctttcggcg
    18241 ctcctcagga tccttgatcc cggtgatttc ggtggtccat ttgaccggcg gctcaccctg
    18301 cgttgcgagg aagtcgagct gcgtctcgtc gagcagctcg accggaagct gcggagcctt
    18361 cgagtccata tcgccgccga gcagcgtgtt ctcgctcgag aactggctgg ggttgtccag
    18421 cttgtgctct ggattcggca ctcgcttgcc gttctcatcg atcgtggact tctgtcgctc
    18481 gtcatccacg ccgagcttcg ccgcgacgcc cttggaggga tccgcatcgt tgcccttgtc
    18541 agccttcttg atcgccttca gctcgtccgc gaattcgggc gcagtcagga ttcccttctt
    18601 ggcgcccatc ttgtcgggcg cagcgttctc tttgcgctta tcgaggttga acggttttgg
    18661 ttccgcaaac ccgttcttcg cagcctcggc cgcttcctct ttctgggtgt tgtcgatgtt
    18721 ctcgctgacc cgccgcatgt acgcgggtgc tcctggcccc atcatctcct tcgcgaggaa
    18781 ctccgagaag ttgatgagcg ccatcgcctt ctgaaccatg cttccggtgg tcagcgcgtc
    18841 gaggacatcc ttgaactctt ttccgacgac gcccgcgaag ccgatgttga ccggcgtacc
    18901 cagcttgtcg ccgatcgcgg tagcccacgc cgggttttga cgggctagcg cttgcagctt
    18961 cacgtcgacg gtcttcgcgc tttcgatgac cttcgtttgc tgctcgagga tcatcagctt
    19021 ggcctgcgtg gccatgcgat tcagaccctc gtgaccttgt ggccacgcgt tcgacatcgc
    19081 cttcacgccg atgaactttt cgaactggga gcggacgttc gtgagctgcg agtcttcgcc
    19141 gcgcgcgtac gccatcgctt cgcgcatctc ggcgaacttc tcctggatct tgatgagcag
    19201 ctgatcgcgc tgcttctgcg tgtccggcag cttctccttg tcgagcgcca gatacttctt
    19261 cgtgagggag atgagctcgt cttttttgcg aatcggatcc ttgctgtccg atttccactc
    19321 gatcatctcg ggcggatcgc tgagcttgag cttcgcaccc ggctcgccct tgccttgcgg
    19381 aaccgtcgcg ttcgtgacgc tcgcgagctc catctcgctc tctgcgcgcg tatgcgccgg
    19441 agcttgcggc ttgtcttccg gtgcaggtgt gcctggggcc ggagcgttcg ggtcggcagc
    19501 cttcggcgcc gccggatcga caacgggcgt cgtggtcggc gcggtcggcg cgacggtgct
    19561 cggtggtgca accggcgcgt tcggggccgc ggctgtcgga tccttcgcgg cgtctgcctt
    19621 cgcaggatcg gcgttcggag ccgcgggcgc ttcggacttt gctgccggcg gcggtggcgt
    19681 ttcgaccttc ggaggcgcag cgggcggtgc gtcggtcttc gatgcgtcgc ctggtttcga
    19741 tgcgtccgcg ctcggcgcgt ccttcgctgc gtcggccttt ggcgcgtcgg ccttcggcgc
    19801 atcggccttt ccgccggcat ctgcgggcgg tgcatccgtt ctcggtgcag gcggagcgtc
    19861 gaccttcgcc gggggcggag gaggcgcatc gacctttgcc tgctgaacag gcgcgggcgg
    19921 aggtgcttcc gtcgacgtag tcggagtcgg cgctccgttt gacgtagccg cggctggcgc
    19981 gactggcact tgactgccgc ccgcggcgcg attcaccgca gggttcgtct tcttcgcgct
    20041 ctcgatcatg agcaagtgga ccttgtccat gagctgttca gcgtcgggga agtcgagctg
    20101 gttgttcttc agcttggtct tgatcgcttc gtcgacgccg cggagcctat cgatgacctt
    20161 gtcgcgaacc ttctcgacgg tcatgccatc gcgcgagtcc tctttgccct cgtcgcgcgt
    20221 gaacatgatt tcggcgtggg tcttgtcctg catgacctcc gtacgccagt acttgcgaag
    20281 ccagttgcgg tagttcaccg ccatcgctgc gatcgcgtcg ttgccgagct cccagtcttt
    20341 tccgtcgtcg aggagacgcg gatgttcctt cgtgtcgatc gctacgtaga gcaaccgacc
    20401 cttttcgaag ccgggcccat cgagctgcga cgcgagcagc gacgatgccg tcagcacgcg
    20461 ctcgagctct gccttctcct ttttggcgcc gtcggtggcg gctttgatct tcgtgacgat
    20521 gtcctgcgcc ttcgcgagga gctgcggcat cgtgaggccg ttgttctctt tgacagcctc
    20581 gatctcgctc gccacgaccg cctgcatcgt cgcgttgtcc tcgagctcct tcttggacat
    20641 cgcggcgccg cgatcgagga cttccttctt cagcgcggtg agctccgcct tgacggcgac
    20701 gagtcgttcg gccgcgaagg cgagctgcgt ttcggcgcgc tctttcgcga tcgcggcctt
    20761 ttggtcgtcc gtctgcggcg gcggcttgag cgcggtgatc ttgtcgccga agttcgagaa
    20821 cagccacgtc gagtacgtct cgtaggtctc gggcggcttc ggtcgcgtgg gcgtcttctt
    20881 cgcagtggtt gggtccgcag gcggcggatc cttgcccgga tctttgcccg gatcttttcc
    20941 gggatccttg cccggatctt ttccgggatc ctttgcgtcg cccgtcggtg cattgccatc
    21001 tttggcggcg accggcgcgg cgggcgcttc tgcatccttc ttcgcggcat cgggagctgc
    21061 aggcgccgtc gacgacgcca ttgtgtttgg cgccgtagga gccgcgtcct ttgcgggtgc
    21121 ggtcggattg ccagcatccg tcttcggtgc ttctggcttc gcgtcggcct tcggcgcttc
    21181 gggcttcgcg tcggccttcg gcgcgtcggt cgctgccgtc ggcggtgcgt tcagtgcgtt
    21241 cggcgcgctc ggcgctgcgg gcgtcgcggt cggtgcggac accgacgcgg gggccgtcgg
    21301 cgtcgcattc ggagatgcgt tcggcgctgc aggcgtgacg cccgcggcgg ccatcttttc
    21361 ctgcgccttc ttctcttcgg cgtcgacgcg agcgacctcg cccggatcgg aatcggggta
    21421 gttcgtcgtg tcgccgaatg ccttgatcgc accggcgagc tcgtgcagct tcgccgcgag
    21481 ttgtttgacg acggcgggac gatcgttcgg cttcgcgcct ggcttgagcg atggatcgac
    21541 gacaaccgcc gcagcttcga cggcggacga gatcgcggcc gtgagcttcg cgcgcttcgc
    21601 ctcgggaacg aggctgagga ccgacttcag cttttgcacg gcgtagccgc ggatactgtt
    21661 gaactcgatc ttgccttcgt cgggcttcgc gacgagctcg tgcggcttgc cggccatgct
    21721 cgtggcaacc gtgatcggtt ccgcgggcgt cgttgtcact tcttccttcg gcgtcgcggc
    21781 aacgtcgccg gcggcgggtg ctgcggcagc agcagcggtg tcttgcttgc gctgctcggc
    21841 ctgattggct tgcgactggc cgagcatgcc ctgctcgtgt gcggcgacgg tcgccacgtt
    21901 cgcggcgtca ggcgcagcgc ctgctttgtg cgccgcggtg tttgcgttct gcgcagcgag
    21961 cactgcctgg tacttcggat ccatctcgac gagctgccac ttcgcgtggt cgatgtcgtc
    22021 ccaggcgacc cgcatcatgg tttcgcgaat gccgccgccg gtcttgcccc agttgttatc
    22081 gcgagcgtcg cgctgcatcc acagcggcga gagcgtgccg tcgaagaccg tcggcttgcg
    22141 aagcttctgg ttcttgtcgc ccgacgtgat cttcgcgagg accgcggtgc gcttgacgac
    22201 catgcgcagg tgacccttcg gataccagtt cgcgtagagc gcgagcagat cgcagtactg
    22261 cgagaacacc gcgtccgact tgatgtcggg ctccgggaac atccagctcg ggttctcgac
    22321 gatcgacgga ttgacgtatg cctggatcgt ccccgacgga tccgccttcg ctttcttcag
    22381 cacccacgcg atcagcttgt ctcccttggg agccgcggtt tgaccggcgt ttgcggggtc
    22441 cgccagccag tcggcttgcg cagcgccctc tttgaagttc ttgccgctga aagcgccttc
    22501 cgtgttgatc acgcgcgcga gcgacgcatc gctcggcacc gccgcattga agttcgcggc
    22561 cgtgagaggc gtgcctctca tgtgatagcc accctcgccc ttgcgctcga ggatgcagac
    22621 catcttcatc acgtcttcga tggacttcgc gttgccgtac ttgtccgcgt ggaacccggc
    22681 gatgacgtcc gccttgaagt gttccttcag gcccaacgcg tccggctcga gagacttctc
    22741 cgcctggacc tgcgtcgtct tttcctgcgc ggtcgccgac atcacgggat gcaggctctc
    22801 gaacgtcggg aagtgttgcg cgcgaagggt tttcggatcc ttcttgctct cgtcgatgcg
    22861 ctcttgctcg ccgagcgttg cgatcggcgc gggaactttg tcgacgacgc ggaagactgc
    22921 cttcagatca tcgtccgctt tcttgagcgc tgcgggatcg accggcgcct tcgcggcggc
    22981 gggatcgggt gcagccggcg gtggcggtga cggcggcgta gccgttggcg acggcgcgcc
    23041 gggcgtgacg ggagccggag cattcgcgac gggcggcttt gcttccgtcg tcggtggtcc
    23101 tgccatcggc gagaccgaca cactcgcggg cggtggtggc ggaggaggag gagcgatgac
    23161 cggcgctgca gtcgatgcag aagcatccgc tgatgctggg tccttcgctg caggcggcgg
    23221 cggcgccgca atcgcgggcg gcgcgggcgg cgtcatcagc tgctgctgct ttttgttctc
    23281 cgcttcgacg cgcttcgcag tctctggatc tgcgtcggtg agcttgctcg tattgccgaa
    23341 tgcgcgaatt gcgccggcga gctccttgag cttgcctgcg agcttcttca cgaccgcggg
    23401 acgctcgctg gccttgccgc ccggcttgag cgccgggtcc gcaaccatcg ccgtcgcttc
    23461 gacagccgtc gctaccgcgt tcaacgcctt ggtcttcgcg ggctcgggga cgagatcgac
    23521 gacagacttc agcttcgaga cggcgaagcc gggaatgctg tggaagtgga tgtctccggt
    23581 cttcgcgtcg gtgcgtaggg agtgcggcgc acccgccatg tccgtcgtct cttcgatcgg
    23641 ctcagccgca tcggccgcga cttcatttgc ttgcggctgc ggcggtgtct tcggcgtgac
    23701 tgcctgcggc ggcgcgggca gtggcggaag cacgagcgct tcgcccgcgg gctcgggcag
    23761 ctccggcgtt tcgggcgcga ccggcaggcc gagagccttg tcgatctcgg cgatcttggc
    23821 tttgatcttt ttgattttct cggcggtgga cttcgcgccc ctgaaggtca ggccgagctg
    23881 aataccggcg acgaccggac ccgcgaacgc agccgcctcg tgtgcgaggt gctgtaccca
    23941 gtgatggacg cctgccatct ctgcgaggcc ggcgccgacc tcgacggtgt cgacagccag
    24001 aggagctgct tcggtcttgg ccgtcgctgc acctgcgccc gcctggaact tggcgacctt
    24061 cgcttccttg tcgatcttga cgatcgcttc tttgaaggag agcaaatccg cgtcgaatag
    24121 atcgccgagc gcctggatct gcgtgggcga cagcatgagg agctggttct tcgcctgctt
    24181 gaggcccctc ttcttcatgt acttggtgaa gaacatgatg ccggccttgc cgatggcggc
    24241 cggaatcgcg atctgcggcg cgatatgggc gatgaggacg gcggcggaga ggcccacggc
    24301 gccacctgcg ttgaagacct cccagccctt gagagcgttc tccatcgcga gatcgagttg
    24361 ctcttcgacc ttgtgcagct cgagatgctc tttctctttc tcgagagcgt gtttctcctc
    24421 gggggtgagc tctttcttgg cttcgggctg cggtccgccg gccgcgggcg gtggcggcgg
    24481 cggtgccgcg ctcttcgcgg tttcggctgc cttcgcggtc tcggcggcct tggcgtcggc
    24541 ggcggccttc gcgtcggcag ctgccttcgc gtcgacggcc ttctggatcg cctcttcttt
    24601 gagcttcttt gcgacgacaa ccgatgcgcg cagcgcagcc ggatcgggca acaggatcgc
    24661 gtcgagccag atctgcacct tcgccatctc gtcggcgatg gcggtgccag gcgtgttgac
    24721 gtcgaccgcg tcgatcgcgg tcttcgctgc cttcagtagc ttgtcgcgat gcgagaggat
    24781 cgacgcgaga gccgtgtcga cctctttgat catgccgttg caggtcgcga cgccgcgaac
    24841 gaacatcgcg tcttgctcga acgccgtgag ccacggcgtg acttcgccct tcagcgcttc
    24901 gacttccttg tagccagtcg ggtcgagctt cggtgctgcg gtcttgacga ggatgctgag
    24961 cgtaaatgcc ttctgcgcct gatcctcgag agccgtgcgg ctgtcggtga tgaacgtctt
    25021 ggccgtcgcg accgcgggat ggctcgcgta cttgccggtc ttcatctgca agccgagctc
    25081 gaatgaaccg agatcgcgga cgagcttgag gacctcgctc ttcgtggcgg tcgcccagcc
    25141 tttgggcttc agcagcgcga tctcttcttt gacgaagtcc tcgagctgct gctcggcgtc
    25201 gtccttcttt tgcgcgtcgg ccgggatcgg cggcggcgtc gcgggcgtcg cgggatcttt
    25261 cgcgtcggcg gccttcgcct tcgcatccgc gggaatcggc ggtggaccgg acttctttgc
    25321 gtcggccggg atcgggggcg gccccttttt ttgctcttgc ccggcctgga cctgcgtcgt
    25381 cttgcccgga tcgagcgacg ccggatcgac gggcggcttc tgatcggtga ggttggcttt
    25441 gccaggctcg ggagatgcgg tcggattctt cgccgcctcc tcgagcatct tgcggaagag
    25501 ggtcgagcgc tgagccggat ccgtcgccgc cgccttggcc tgctcccaga gcttgccggc
    25561 gatctcgagc gagacgtgct tgtccgcggc aaggacacgc cactcgatcc atgaaccctg
    25621 tgtcgcagct gccactttcg ccatgatacg ggactgccgg ggccgatttc ccccgcaaag
    25681 gcgcagtttt gtccccgctt ggagctgccg tttcgacgcg cgaagccatg agggatatcg
    25741 cggtcctagc gtgcgtgcga ggaactcgtt tgcgaggtcg gagtacgatg caggcatgtg
    25801 gacggtagcg atcgcgctcg cggctatcgt cggcgcggga tgcgggcggt tcggctttgg
    25861 cgccgatgac ccgggtcggg acgctccgca agtgggtgac gggggcaccg acgtggtgac
    25921 gatgggcggt gatggcggcg tcgatgcgcc agagttcttc gcgtgcacgg gcagtgaagt
    25981 ctgcgaagga ttcgagagcg gcttcggcgt ctggatggcc gatgcgatgg tctcgctcga
    26041 cgcgacacgc gcgcatcgcg gcaaccactc ggtgcacgtg cattcgccgg cgttcgcggc
    26101 gggtggcagc agctaccagg cgatctacca gtcgcagacc gtgagcgcga acatcaaccc
    26161 gttttgggtg cgcggctggt tttggttgtc agcgcgcccg aacttcgaca acgcgatgga
    26221 gctcatgacc gcggagcggc ccagcaccag ctcgggcgac tacgtgttcg tgcacgccga
    26281 ctcgacgaac gtgtattcgc agttcgacgc gcagatcatg caaacgtcga cgctcgtacc
    26341 agtcggtcaa tggttctgct tgatctggaa ggtcacgcgc aacaccggca cgtcgggcgc
    26401 gctcgagctc agcggcgacg tcacggtctc gctcgcgaac acgaagacag attcgacgac
    26461 gtcgtcgctg cagatcgtcg tgctcggcat cggttatgcg tcgagcaaca cgccggtcgc
    26521 gcaacccgcg caggacttgt ggatcgacga cgtgatcgtc agcgccagtg ccgtgacgtg
    26581 tagcgattag ctactgctcg gcgagctgct gcaggtagct gaggagctgc ggcttcatcg
    26641 cgcgcatggc gcgtgcgcgg tgcgagagat cggacttggg gccgatgcct gcttcggcga
    26701 acgtcatgcc gagctcgggc gcgaagaaga gcgggtcgta cccgaagccg ccggtgccgc
    26761 gcggtgcggt gaggattctg ccttcgcagg tgccgtcggc ggtgatgacg cgatcgcgga
    26821 gtgggccggt gacgtcggcg agcgcgagcg acgcgcggaa gcgagcggtg cgctcgtctt
    26881 gcgcgacgcc ggtgagctcg gcgaggagct tcgcgttgtt cgccgcgtcg tcgtgatcgg
    26941 ggcctgcgta gcgcgcggag atcacgccgg gcgcgccgct tagcgcatcg acttcgaggc
    27001 cgctgtcgtc ggcaagggca gggagcttcg tgaacgcgga gacctcgcgc gctttctttg
    27061 ccgcgttgcc cgcgaaggtg tctgcgtctt cgatcacttc cgggatcggc gcgccaatgc
    27121 gctgcgcggc ttcgtcgatc gagagcaccg cgatgttggg caggagctcg cgaagctcga
    27181 cgagcttgcc tttgttgcgc gtcgcgaaca cgagcgggcg cgcgctcata cgccggcggc
    27241 ctgccgctgc gcgacggtga gctgcttgat gcctgcgagc gcgaggtcga gcatcgcgtc
    27301 gagctgcttg cgatcgaacg tgtcgtgctc ggcggtgcct tgcacttcga tgagcttgcc
    27361 gctctcgtcg ccgacgacgt tcatgtcgac gtcggccttg ctgtcttcga tgtacggcag
    27421 atcgaggacg acttcgccgc cgacgatgcc gaccgagact gcggcgacgg gcgggcgcag
    27481 cgcacgcatg tccgcgagct ggcccgctgc gacgagcttc ttgatcgcga gcgcgcaggc
    27541 aacccatgcg cccgtgatcg ccgagacgcg cgtgccgccg tcggcattga ggacgtcgca
    27601 gtcgacgttg atcgtccgct ccccgagatg ctggagatca acggcggcgc ggagcgagcg
    27661 accaatcagc cgctggatct ctttgccgcg gccacccgga tcgcgcttgc tgcgcgtgtg
    27721 cgtcgagcga ggcaacatcg cgtactccgc ggtgagccag ccttggttct tgccgacgag
    27781 gaacggcggc acacccttct cgacgctcgc agcgcacagc acccacgtgt cgccgagctt
    27841 cacgagggcc gaaccttccg catgtttctg aaagcccgtc gtgatctcga gcgggcggag
    27901 ctggtcggga cgtctaccgt cgggtcgcat cgcgcggagg ctaccctgcg agcgacttct
    27961 gcgcgaagct cttcgcgatg cattcgaggt ggcgtgcccg acgatccgga taagccggtc
    28021 gacctcggca acgcgccgac gacgcctgca ttggaaggga cgatcgctgc gccctcgccg
    28081 gggccgttcg cgcaggggag cggcaatccc gactcgacgc tcgacatgcc ggccgcggag
    28141 gctgcggctc tcgccgaaaa tcagatcgcg cactttcgcg tgatccgccg gctcggtatg
    28201 ggaggaatgg gtgtcgtgtt tgcctgcgag gacacgaacc tcgcgcggca agtcgcggtg
    28261 aagctcgtgc gcgacgagaa tcccgcgtat cgcgcgcggc tcttgcgcga ggcgcaggcg
    28321 atggcgcggc tcgagcatcc gaacgtcgtc cgtgtctacg aggtcggcca ggatcgaggc
    28381 cgcatgttca tcgcgatgga gctcgtcgac ggcgagacgc tcacgtcgtg gctgcgatcg
    28441 tttcgcacgt ggcaggacac cgtcgagatg ttcatgcaag tcggcgccgg gctcgcggcc
    28501 gtgcatggcg ctggactcgt gcatcgcgac ttcaagcctg acaacgtgct cgtcgacagg
    28561 cgtggccgcg cgcgggtcgc ggacttcggg ctcgcacgcc tcgacgcgca aggcgggcct
    28621 tcgccgatgg cgcagccgat gacgaagagc ggcgtcgtga tgggcacgcc gggctacatg
    28681 gcgccggagc agcattttgg tgccgacgtc gacgcgcgcg ccgatcagtt cgcgttctgc
    28741 atcgcgctgc gcgaagcgct cggcggacgg cccgtcgacg agcggcgctg ggcacaagtg
    28801 cccgagtccg tgcgttcgat cgtcaaccgc ggcctctcgt acgacgtcgc cgatcgattt
    28861 ccctcgctcg atgcgttgct cgtcgcgctg cgcagcgcgc ttgcgccagt gggagtcacc
    28921 gacgaggttc gcgctgcgca tacgtcgtcg cgaggccggc cttcggcgcg tgcgcgttcg
    28981 cgctcgatgg tggacgacgg catccgcaag agcgagccgc gctggatcgt attgctgctc
    29041 gtgctcgcga tcgtcggcgc aaccgcgggc atcgtcgcgt ttgtcggtac gcggaagaag
    29101 ccaacgccac ccgcgcggcc gcccgtcgcg accatcgcgc ccgcggatgc cgctgttgca
    29161 ccgccgatcg ctccttcgat cgatgctgcg gttgcgcagg tgcaagagcc gctagcgcct
    29221 gatgccgcag tgacgatgct cccgccgacg aaaacggtcg tgcgcacgcc gcgtgatgcg
    29281 ggtatcgatg cgatgcagca gcaagtggtc gcagccgcgc cgggacagct tcccgtcaac
    29341 gggaacgggc agggcggtgc acctgcatgg ggcccgtact ccgccgcgtg gcaaaacgcg
    29401 aagggcggca agacggacgt cgtccgcgac gcggtgaagg acgtcggcta cgacagcttc
    29461 gacgcgagct ggaagaaagc cgatctcgag aagacgatcg aaggcgcgac cggcgccgag
    29521 aaagcgatcg cgcaggtgaa gctcggcatg gtgctgcgcc gcgcgggcga ctgcaaagct
    29581 gcgagctcgc agttccaagc cgcgatcaaa tccctcaagc ccttcgacga cgagtccgaa
    29641 ggcaagtggc gtggacgtgc ggccgtcgcg cggtcgctgt gcacgctcgc cgctggcgac
    29701 ccacgccttg cgcgcgcgca cttgatggat gccatcaaca cgttccccgg cgagatccag
    29761 ctcgtgcacg gcatctcgca gttcgaggac aacgcggatc gcgcgctcgc gtacgcgcac
    29821 ttgatcaccg cacagaagca aggcgacgcg agcgtgaaag ccgccgtgaa gatctatctc
    29881 ggcggttacg ggctgtcgct cggaccttag gtcccgatgc ccagctcttt gagcagacgc
    29941 tttgcgacgt cacgcaccgc gaacggatag atcgtctgca tcgccgccat cttcagctcg
    30001 tgctttggca ggagccgcag gatcttcgtg atctgatccg tgccgaggcg cggattcgtc
    30061 aggagcgcgc ggcgcacttc gggaatctgc agccagccgc cgttgccgag gatgatttcc
    30121 atgagcggcc gcggcaacgc gcccatgcga gcgatgcgcg cgacctccgg gcccgtgagc
    30181 cgcgggttgc gcagcagccc ttcccagacc gccttgccgt acatgcgctc gaggacgatg
    30241 cgctcggcga cctcgccgtt gtgcgccatc ttgatctgct gcgcgagcgt caggccgcga
    30301 agccgctcgt gaacgttgag tggcgctttc ttggcgtcgg cgtcagcgtc ctctgcatct
    30361 tcgtcgacgg tctcgtcggg atccgccggc gcgtcgatcg cgggctggcc aggtgtgtcg
    30421 gcggtggggc tatcgcttgt cgtcgcggat gccgcggccg cgaggctcgt gatcttctgc
    30481 ttcatctcgg catcgcagcc gaacagctga aggccggagc ctttatcgcc gttgacccag
    30541 acgcacttcg cggacacacg cagctcgtcg ccggagcctc gcagatgcag gtcgacgtct
    30601 tcgcccatgt cgagcacatc ggggacgagc acgccgccgg ccgccaggtc acgcgcgagc
    30661 gccgcttggc ctgctagctc gagaacgaca gtgcgcgcca ccgccgcatc ataacgctag
    30721 tcggggacga gcacgccgcc tgcggccagg tcgcgcgagc gccgtcataa cgctactcgg
    30781 cgccatgctg gtgacggcga gacgttgcga ccgcgacgcg aacgacccac ctacgcgatg
    30841 ctgctggagc ccgcaggcgc gccgcggcta taggccgttc gcgatcgcct ttaggatctg
    30901 atccgcgatg ccggctccga cgcccgtcgg gatgttgttc acgtagcgat gtgccgagac
    30961 gcggcatttt tcgcgagcgc gttcgtcggt aggggactcg accgcgcgga ggcaggtgat
    31021 gcggacctgg agccacatcg cgtccttgta cgcctcgtct ttcgtgcggc cgttgatgat
    31081 gccctcgatg gagtgctcgg cggagccatc gctcttctgg aggtgctgct gaaccgcgat
    31141 gctgtacatg atgcgcgact tatccgcgtc gttcgtcgcg gtcttcagct ggtcgatgta
    31201 gtccgtcatc gtgccggaga gcggattgag ctcgacggcg aggatggcct tcttgatatt
    31261 cgtcgcgccg gccttgtgct tgccgttcgc gctgcctgca ccgctgccac tcgcgctgcc
    31321 gttcgtgttg cttccgatcg cgctgcctgc gttcgtcgtc gcgttgctgc cactcttcgg
    31381 ggtctcgccg atcggcttcg gattaccgat ctcgatgtcg tcgccggtgc cgttcgcgcc
    31441 ggtgttctgc gcgacgaccg tcgttgcgct gtcgagttgc gcgagcgtga tcgtgccttg
    31501 ctccgtcgtc cacgcgttgt tcgcggtgac ctcgaagtct ttgcctgcgc gcttcacctt
    31561 cacttcgccc tcggtaacgc gcacgtcgac gtggcccttg ccgtcgtagt cgacgctgaa
    31621 cttcgtgccg acgtcctcga tctcggtatc gcctgcatgc acgacgagga ggcgattcgg
    31681 atcatgcttc acgtcgagcg agagcgtgcc cgcgcccatc gcgaggtcgg tgcgcgttcc
    31741 cgtgtgcgtg acctccacct tcgtaccgtc ggtggtcgac agcgtgaacg gtgcctcggc
    31801 gaccgtcggc cgtgacggcg tcgacgcggg cgcatgcagc ttccacccga cggcacccgc
    31861 aactgccatc gccatcgcga cacccgcgag cgcgaggccg cgcctggacc acgagctctc
    31921 gcgctgcggc gccatctcac tgacggcgac gacgagcttg cgctcgatgt tcgtcagccg
    31981 ctcgtcatcg agttgctcga cgggaacttt ccctttgagc ttcatgcgcc ctccttcggc
    32041 agctcgatga ccgtcgcgcc cgacgagccg gccgacggct gtacgaactc gctcaagatc
    32101 ggatctgccg ccgccgcttc ttcgaccttc ttgcgtgcgc gccacacgcg cagcttcgtc
    32161 gcggtgacgg acgagccgac gagcttcgcg acttccgcca tcggacggcc atcgatctcg
    32221 tgcagcgtaa acgccaagcg cgatgctgcg ccgagctcgt cgaggacggc atacaggcgc
    32281 ttcacgccat cgcgcgccat ctgccggctc gggctcgcgc cttcgccgcc gggcgttgcg
    32341 tcgtcgtcgc ccatctcctc ggtgacgatc cgccgcccct tctgcgagag gtagcgatac
    32401 gcgcagcgca cggccacgcg atcgatccac gtcgagaggc tcgcttccgc gcgccagccg
    32461 cgcagcgact ggaacacctg gataaacgcc tcttgcagta gatcgtccat gtcgcgatta
    32521 cttccgagca cgcggtacaa gctcgcgtgc acgcgaccgc ggtgcttgcg aaacagctca
    32581 cgcgtagcag ccggctcgcc ggtgagacac cggtcgacga gcaggacatc ttccacgtag
    32641 gccgcagcca cggtcactgc ttagatggtg caccggatcg gcccgccggt tacacgctgt
    32701 tcaacctttt gttttcgctc gcgtcttcag ctgcacgagc ggcccggggc actcctcgtg
    32761 cagcttgcac gcgtcacact cgccgcccgc ccaaatgcgc tcgtagaggc cctgcacctg
    32821 atcgaggagc tgcgcatcgt cggtgacaaa gccgagctcg aagttgcgac gcccgctgcc
    32881 cttcgcgccg aggcctgcgc cggtccagtt cgcgctgcct agatacagca gctcgccatc
    32941 gacgatcacc gccttgagat gcacgcgtgg gcatcgccgc aacgcgaggg cgccgccgcc
    33001 cgcgagcttc ggatgctgcg cgagctccgt gcgaaacggt cccgacggca gctccgcatg
    33061 caataggcgc agctcgacgc cgcgcgcggc cagctcatcg agatgcgcga gcacagaaac
    33121 gtacggccgc ttcttcatcg agcgccgctt cccgggccgc gcgcgatgat cctcgaccat
    33181 cagctccttc acgttcgccg tcgcgatcca gacgctcacc ttcgcgtcga gcaccgcacg
    33241 cagcacgcgc tcgtagtggc cgcgacccga cacgagctcc atcgagatcg cgcgcgccgg
    33301 caacgcgaga tcgagcggcg acccgccggc ggcttcaacc agcgcgctcg cgcagctcat
    33361 cgcgctcgtg tcgcccgccc cgcgggcgtc cggcgttgcg atcgcacggg ctacgtccgc
    33421 tagcgaagtt ggctttcgct tcgcgcgacg tgttgcccgc tgcgcgtcct cgagctcggc
    33481 cgcgacgtcg gcgagcgatg agccgttcgt cgtcgcgcga cggccgtcac gcacggcacg
    33541 ttgcgcggcg tcgagctcgg ccgcgatgtc ggcgagcgat gaccttgtcg tcttcgcgag
    33601 cgatgagctt gtcgtcttcg cgagcgacga gcttgtcgtc ttctcgagcg atgagcttgt
    33661 cgtctttttc gcacggcgtg cagtgcgagg tgcactcgcg atgtcccgcg cgagcgcgac
    33721 cagttcggcg gcgttggcgc cacgcgcggc gctgcggcgg cggcgcgcca ttaggcgccc
    33781 atcagcgagg gcgtcgacac gcagtggccg ccatcgacga ccatgatgtg gcctgtcacg
    33841 tacgccgcgg cgggcgagcg caggtagacg gcggcggcgg cgatttcgtc gattgtgccg
    33901 aagcggccgg ccgggattcc g
//
LOCUS       DATLBZ010000216        17493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_962196, whole genome shotgun sequence.
ACCESSION   DATLBZ010000216 DATLBZ010000000
VERSION     DATLBZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17493
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962196"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VMZ53_11480"
     CDS             complement(<1..818)
                     /locus_tag="VMZ53_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HUS29129.1"
                     /translation="MPPALFHRLLIANRGEVAVRVARACDSMGIVPVFAVSEADREAP
                     YTRGREVVVLGPGRPALSYLDVERIVQAAVQARCTALHPGWGFLSENPLLATLCAQHG
                     VTFIGPPPHVTSLMGSKTRAKAAMRAAGLDVIPGSNGVLADAADALRVATATGFPVLF
                     KAENGGGGRGMRIAKRAEEVEQCYAEAQQEARAAFGGDRVFMEKLVEGGRHVEIQVFA
                     DRYGRAVHLGERDCTVQRNHQKLIEESPSPSLSADLRASVCERAARAAASIGYVG"
     gene            complement(845..1330)
                     /locus_tag="VMZ53_11485"
     CDS             complement(845..1330)
                     /locus_tag="VMZ53_11485"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HUS29130.1"
                     /translation="MIEVGQKIPSVKVKRANEEGGEEIDTAAFFAGKKVILFSLPGAF
                     TPTCSQKHLPGYVKELPQLRAKGVDMVACLSVNDSFVMRDWAKQHDALGKVEMIADGN
                     ADFSKALGIETDLTKANMGIRCRRGLIILEDGVVKSIEMEAPGKFEVSSAEACSLKLG
                     A"
     gene            complement(1336..2208)
                     /locus_tag="VMZ53_11490"
     CDS             complement(1336..2208)
                     /locus_tag="VMZ53_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HUS29131.1"
                     /translation="MPTHRRFDRAARLLGDEGIARLAGSTVSVFGVGGVGSFAAEALV
                     RSGVGRVILCDYDRICVTNVNRQIHAMKGTLGKPKVEIMAERLRLINPDAVIETRAEF
                     YSAETSARLLVPEPDVVIDAIDNIAAKMHLIATCVRDRLRIVSAMGAAARLDPTAVRV
                     ADLSQTKIDPFAKDLRRNLRAKHGLDCAKRTGVWAVYSEEPPQAPQSLAYDDGAFRCV
                     CPGGMNGVNDCEHKSRVEGSVAFVPSVFGMTMASVAVKLLCGLPLPRTCDEPAPPRVT
                     VRPSRGPADEIRGN"
     gene            complement(2219..2998)
                     /locus_tag="VMZ53_11495"
     CDS             complement(2219..2998)
                     /locus_tag="VMZ53_11495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HUS29132.1"
                     /translation="MIDTHCHLDLERFDADRAEVIARAEAAGVRGVLVPGIRPATWRA
                     LVELGRSHVMLRVALGVHPQIVPELAEAEVGDLAAQIIEATREAGALAVAVGECGLDG
                     GTGEHARQEELFRAHVRAARELKLPLVVHVLRAHDVAPRILREEKASDVGGVMHSYSG
                     GAALVPIYRDLGMAFAFAGPVTYPNARRPLEAARVVPGELLLAETDAPDQAPAAHQGS
                     RSEPAYVAEVIGGLAAARQTSPEEMARLTTENARRIFRWPP"
     gene            complement(3015..3353)
                     /locus_tag="VMZ53_11500"
     CDS             complement(3015..3353)
                     /locus_tag="VMZ53_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29133.1"
                     /translation="MLDERMLEVEGSPAARAAVIARALAGDRHRTIAFLAAYLAVTPR
                     AADVGPVIRQLIRCHERPDLAPTHLRRLAAIVHVACAVAEDREPEVAAVTAAGITREL
                     PIWRKVAARL"
     gene            3412..4347
                     /locus_tag="VMZ53_11505"
     CDS             3412..4347
                     /locus_tag="VMZ53_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HUS29134.1"
                     /translation="MVRKTLKGWSHWWRTSESVLARWLRAAHEALLGEMPILAAGTAL
                     FAIIAVVPTLAAAVALFGLMADPSEIAGQLTALRHVLPVEVVDFIANQLTRQASRGTT
                     ELGLQLGISTFLAVFSARGSARALIDSLNRAYRVRELRRPLARFALTLLMATATLVGL
                     ITMFAVLVALPTIVGAINKHWIAWAIVLRWPALLAVVFLSLLALYRYGPSPRPLGPKE
                     RIETPISLPPHPMRHVCLPGAVIATVLLLIVSWGLSLYVDNIANTELVYGAFGSVIVT
                     VLWFYFSTMALLIGGFVNAELERHRGAPQPDREMY"
     gene            complement(4351..5394)
                     /locus_tag="VMZ53_11510"
     CDS             complement(4351..5394)
                     /locus_tag="VMZ53_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29135.1"
                     /translation="MMKRSLFSTFSFVLALGATAAGCAAQAPGEDDDPGEGSGSGSGS
                     AEQPPLTAEGKYALVSDFDIATNMPGTAGEVVNMFIAATDDADDPAHWILDQLVAKLP
                     DGTFKNIVKGAIPFVSGYLNDRLLQVAPDFVVTIRDMGNKFGQAARHFGTLETLDVPA
                     NGMATHTMNGVQFTVDQIELQYMFKDYQMQDVAVPNVGVQVDKLGKLTVADHKLPLSY
                     GKVMRIAMDEVIIPLVDPSATNLEDILTGLVNCHAVGQYVYEAVGIGAPSTFEAACTA
                     GLHGGSQVIYAQIGKIDSSALEFNVNGVAKGIDKNHDGKMDSIQVGAWAGTLSYGGNG
                     APLAHGQFTGQRL"
     gene            complement(5628..6695)
                     /locus_tag="VMZ53_11515"
     CDS             complement(5628..6695)
                     /locus_tag="VMZ53_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29136.1"
                     /translation="MKTVKTLSLALALAIGSAVTGCAADSPDDMGGGGGGGGGGGGGD
                     DVPRALDASGKYQMRSTFDIATNMPGKVGEVSNAIIAATDGPADPTEWILDQIIAKMP
                     NGTFKNLLSGAKPFVAGYLNQRLLTIAPDFVSTMVQTGHDFGVMAKNFGLNEQLEVAQ
                     SGTDYNSTVTALGVHFKIDTLETDVNFADHQIANVAAQNVGVKIDLTGKLDIAEHKLP
                     LSYGKVLRIGLDEVIIPALDSNATNLQTLLADLVNCQAVGVQIDAALASQFGFGGGAS
                     FWQSACTGGLQYGAQTIYSKLDGVDASALEFGLVGTAKAVDTNADHKVDALQTGKWTG
                     NLSYSGTPAPLAAATFTAQRM"
     gene            complement(6930..8927)
                     /locus_tag="VMZ53_11520"
     CDS             complement(6930..8927)
                     /locus_tag="VMZ53_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HUS29137.1"
                     /translation="MRTAKLFPILLLAACGGSKPAPVPAPPPDQTTGDTAQTPPPDQT
                     QPPPPAGNDDAKLIAEAKQFVADADKQLRQLFTDSSVADWANQTDITPEHEAAAAKAG
                     EDLSNGITRLIKASRKFEPIMGKLDADTRRQLTLLKFSGQPAPDDPKQAEELAKLGAE
                     MTSIYGKGKVCDSKHQKELDAAGAKVVSAKDDKGKAAAQKALSKVEGKYCKDLDALSK
                     VLQKSKKPAELLAAWKGWHDNVGRAERPLFVRYVELANAGARGVGFKDVSSMWRSSYD
                     MPEEKFEQEVDRLYEQLKPLYAQLHCYTRRGLNKKYGDKVQPKNGPIFSHLTGNMWAQ
                     GWEYLYSDLEPYKGVAQVDVTSTLEKKFDPIKMVKMGEAFYVSLGMNPLPESFWQKSQ
                     FTKPAGKEVVCHASAWDVTLNNDLRIKMCINKNQEDLVTIHHELGHDYYYNNYYKLPV
                     LFQSGANDGFHEAIGDTIALSMTPEYLHTKGLLDKVEKNDKATINAQMHKALESIAFL
                     PFGLLVDKWRWDVFSGKVKPDEYNKHWWDLKLKYQGVVPPLPRTEPELFDPGAKYHVP
                     NNTPYMRYFLARVLQFQFHRALCKKAGWTGPLHECSIYGNKEAGAAFAKMLAMGASKP
                     WQDALFELTGQREMDATAMVEYFAPLQKWLEEQNKGDQCGW"
     gene            complement(9016..9564)
                     /locus_tag="VMZ53_11525"
     CDS             complement(9016..9564)
                     /locus_tag="VMZ53_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29138.1"
                     /translation="MRFVLSIALALAGVSACKEKSEVIEPGGPPPLPPASGTAVGYLV
                     DASGDLQLSGEQLEKLKKIDNSLAAQNGSLEAQIRQIEQPMPAEELSPQQQKAGEKGQ
                     RYDNAPGKSTMQTADSQRLHKMHDDNERDALKKALALLDAKQLEKAKRILQDRGVDVP
                     GEQKQQAPDSSDDGTPLPGMEP"
     gene            complement(9581..11467)
                     /locus_tag="VMZ53_11530"
     CDS             complement(9581..11467)
                     /locus_tag="VMZ53_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HUS29139.1"
                     /translation="MRWALAAAFVVFAAGCPSSQDPDLTGTPDAGMDAGSGETCAAVP
                     TCTTTIRFKGNASAVSLRGDFAADGWTTGIAMTKGADGSFEATLPVTDNQIIVYKLVV
                     DGNWIADPENPRKSPDGYGAFNSVARADCDKCPARPAMEWSDAIMYFVMIDRFADGDS
                     TNNAPISGVEQPGQYQGGDFVGLKQKIEAGYFNDLGINTIWITSPLDNADNANPGSDG
                     HSYSGYHGYWPKDLSKPESKLGSEAELKALVDTAHAHGIQILIDYVMNHVHSASPIYA
                     QHPEWFWPNDNGFGGNCVCGGGCDWGNDRLKCWFDPFLPDFNFQNADARKWSVDNAVA
                     WAKRMGIDGFRLDAVKHIETSWLTDVRARLNAEVAFDQKFYMVGETFDGDRGIIKSYV
                     NPNTMLDGQFDFPLRGQILGTLLRRDGSMNDLAGFLDANDNYYGQGSVMSTFIGNHDV
                     PRAIELALDNPMFGAWDGGKNLAWNGQPSLPTSANPFQRLVVAYTLLMTIPGIPMIYY
                     GDEVGMPGAGDPDNRRFMQWDNYTANQTMLRDRIAALAKLRSAHASMRKGSRQTLGVS
                     QDVLVYKMTMPGDSVIVALNRGDNSQQATNLPTGTYTDLLSGATVTAPLTLEARSARV
                     LAPQ"
     gene            11653..13233
                     /locus_tag="VMZ53_11535"
     CDS             11653..13233
                     /locus_tag="VMZ53_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HUS29140.1"
                     /translation="MLRRGSLVIVLALTSRALAQGTEMPQEPGAGSGSGSGSGSATTS
                     VPEQPPPSQPTAPDDSAPAPVKPERADEPPQDLLKLFGPTPHEAPTSDSGFRFGSYGR
                     VGVGTDLRGGKPEKISLVAHGPRIVEPSYLELELSYGFVRQHADTTEPIILRPIITIA
                     FDDTLFHETGQFDAQPALRNMYLDAKVYNHLSLWCGSRMYRGDDIYLFDYWPLDDQNL
                     VGAGAEVTQLNDLSFAAAVGANRLEHPFQHQEIDVANPVQGATTVEQLNRQRVVVSGS
                     TTYIHMPENATWGAKAKLHAEIHSLPSGTRKREDGTFEALPHDTGELIGLEVGGFQNE
                     HDGLRRHLNGFIRYAKGLAAFDELAPPTTFGPELKTSNASELTFGLSGNYDHELGQLM
                     LGALSRRFIDADADATDADDGWEYAVDVRPLARVVRDVYVGADISYQARFPRGLNPIT
                     LRAEDPAMFQIAPMIAFSPMGPSAYDRPQLRLVYRYAHLNDAALDSYVPDDPRHAHAT
                     VHYLGVQAEWWFNSTTYR"
     gene            complement(13234..13845)
                     /locus_tag="VMZ53_11540"
     CDS             complement(13234..13845)
                     /locus_tag="VMZ53_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29141.1"
                     /translation="MRSIAVCIGLLLAACSINHKSDAYSCELNTDCDSGRVCSGGFCV
                     VAGSQIDAPRLDGPRPGDANQGCPAACSSCNVQQKTCSINCMTANCQNTVVCPAGYKC
                     DISCNTDNACRNGINCTMGASCNIECTSRSSCENVQCGAGPCAVACSGPQSCKNVACG
                     NSCACDVLCTGSQSCQQGINCSSLACRSGLGCTSVPALCHSCQ"
     gene            complement(13842..14813)
                     /locus_tag="VMZ53_11545"
     CDS             complement(13842..14813)
                     /locus_tag="VMZ53_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29142.1"
                     /translation="MKRIVAAAVLVVFAFASTASAAPKAILVLKAEGTADASTRTNLD
                     ASVLKLAKNLDGKVEMGDVTLSDAAAIVGCNPSEASCKDDVLSTLGVDEIVATTATSV
                     PTGTNVTVRRLAKGSAPRAAQSVQPAGKPSDAKLTQDLGPLFGVSAVSTTPAPDKTPP
                     PITPANPPPEAAPPPSNPTYDAPPVQPQPDPSVSAAPAGQVAPMQQDQPQRSYRWQKI
                     GMGVGGTLVVLSFLMWSKAADTQQLIDESPANTPADFVKLGQLEKDADGYASGGNLFF
                     LTGVVLGTVSAYSYWRGHRRARAQTARITPTLTPNSAGIVLTFGGAP"
     gene            complement(14810..17191)
                     /locus_tag="VMZ53_11550"
     CDS             complement(14810..17191)
                     /locus_tag="VMZ53_11550"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29143.1"
                     /translation="MAAADTLRQLLDAGKSSKRLDELSSARVIGKVALQVHAAQQKAG
                     AGKAVGPITPTAITFAPSGEVTFSPADTSSLGYSAPEQLTGGGDRKSDVFSLGTVLWE
                     VLTYQRLFDAMSDAAVRAAVQERDITPPTELNANVPAELSAICMRALARNPSDRYQSL
                     KAMAVEIEEFLSEAGYDDNDERIAQYLKAMPRTSPGVALASKLASAASSGTAVGVPAA
                     PAPTPAAAPAPVPAPAELVPMAPVLPSASTSIRNDKSTISVSVPPQVKVATNGAAVVE
                     AKHEPSKPHALIVEDKPKAVIVDDKVSTSPGLGQTIREPAKPAEKIETNGTPTVELKK
                     PLEAPPVVAPVVKVEAKADVRDTLPDAKADVRDTQPDAKVEEKKPDDKKATKRTGPHP
                     VAAVSLGPAPRESKGDVLAGWGWGTDKHEALAPEGYAHDDEDFHAPPDNKKTLMYVIG
                     GGVAVAALVTIIALVAGGSSPKKKSNRPVAAAQTADAQQAATEPTPTPLPATDTGSAG
                     SAVDPAATNTAATDQAASDQAAAQKAADDKAAAEKAAADQAAAEKATAEQTAADKKAA
                     EKLAAEQAAADKKAAADKALLDKKAAEQAAADKKAADKAAAAQALADKRAAEKAAAEQ
                     KAADKKAAADKLAADRKAAQEKAAADKKAAAEKLAAERAAKQNTRPTTTTTAKTNTKV
                     ATADTKMTPKVDTKTTAKTDTKNTAKVDVESAYRQGLQQFARGDTAGALASLRTSLAA
                     NPNYAPTWRGLGLVFEKMGETQQARAAYKRYLQLAPGANDSEQIKGRLERLGS"
     gene            17276..>17493
                     /locus_tag="VMZ53_11555"
     CDS             17276..>17493
                     /locus_tag="VMZ53_11555"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUS29144.1"
                     /translation="MIMSTSTLTRRAFLGAELPADADAFTPDGMRVAGVVENSMAMRA
                     GLLPGDIVTSIAKQPTRNLCELSHALRVA"
BASE COUNT         2707 a         5780 c         5722 g         3284 t
ORIGIN      
        1 ccgacgtagc cgatgctcgc tgctgcgcgc gcggcgcgtt cgcacacgct tgcgcggagg
       61 tcggccgaga gtgaagggga ggggctctcc tcgatcagct tctggtgatt gcgctgcacc
      121 gtgcagtcgc gctcgccgag gtgaacggcg cggccatagc gatctgcgaa gacctggatc
      181 tcgacgtggc ggccgccctc gacgagcttc tccatgaaga cgcgatcgcc accgaatgcg
      241 gcgcgcgctt cttgctgcgc ttcggcgtag cactgctcga cttcttcggc gcgcttcgcg
      301 atccgcatgc cgcgtccgcc gccaccgttc tccgccttga agaggacggg aaagcccgtc
      361 gccgtcgcaa cgcgcagcgc gtcggctgca tcggcgagca caccgttgct gccgggaatc
      421 acgtcgaggc cggccgcacg catcgcggct ttcgctcgcg tcttgctgcc catcagcgac
      481 gtcacgtgcg gcggtggacc gatgaacgtg acgccgtgct gagcacacag cgtcgcgagg
      541 agcggattct ccgagaggaa gccccagccg ggatgcagtg ccgtgcagcg cgcctgcacc
      601 gcggcttgga cgatgcgctc gacgtcgagg tagctgaggg cagggcggcc gggcccgagc
      661 acgacgactt cgcgaccgcg cgtatacggc gcctcgcgat ctgcctcgct gaccgcgaac
      721 acgggaacga tgcccatgga gtcgcacgcg cgcgcgacgc gcacagcaac ctcgccgcga
      781 ttggcgatca acaatcgatg aaacagggcg gggggcatcc gcgcgtcagc ctatcgcaaa
      841 cgcgctacgc gccgagcttg agcgagcagg cctcggcact cgagacctcg aacttgccgg
      901 gcgcttccat ctcgatcgac ttcacgacgc cgtcctcgag gatgatcagg ccgcggcgac
      961 agcgaatgcc catgttggcc ttcgtcagat ccgtctcgat gccgagcgcc ttcgagaagt
     1021 cggcgttgcc gtcggcgatc atctcgacct tgccgagcgc gtcgtgctgc ttcgcccagt
     1081 cgcgcatcac gaacgagtca ttgaccgaga ggcacgcgac catgtcgacg cccttcgcgc
     1141 ggagctgcgg cagctccttc acgtagccgg gcaggtgctt ctgcgagcac gtcggcgtga
     1201 acgcgcccgg aagcgagaac aggatgactt tcttgcctgc gaagaacgca gcggtgtcga
     1261 tctcttcacc gccctcttcg ttcgcacgct tgaccttgac gctcgggatt ttctggccga
     1321 cttcgatcat gccgctcagt taccacgaat ctcgtctgcg ggccctcgtg acgggcgcac
     1381 agtgacgcgc gggggagcag gctcgtcgca cgtccgcggg agcggaagcc cgcacagcag
     1441 cttcaccgcg acggacgcca tcgtcatgcc gaacacgctc ggcacgaacg cgacggagcc
     1501 ctcgacgcgg ctcttgtgct cgcagtcgtt cacgccgttc atgccgcccg ggcagacgca
     1561 gcggaatgcg ccatcgtcgt acgcgagcga ctgcggcgct tgcggcggct cctcggagta
     1621 gaccgcccac acgcccgtgc gctttgcgca atcgaggccg tgcttagcgc gcaggttacg
     1681 gcggaggtcc ttcgcgaatg gatcgatctt cgtctgcgag aggtccgcaa ctcggacagc
     1741 cgtcggatcg aggcgcgcgg cggcgcccat cgccgagaca atgcgcagcc gatcgcgcac
     1801 gcacgtcgcg atgaggtgca tcttcgccgc gatgttatcg atcgcgtcga tcacgacatc
     1861 gggctccggc acgagcaagc gcgcgctcgt ctcggcgctg tagaactcgg cgcgcgtctc
     1921 gatcacagcg tctggattga tgagccgcag gcgctccgcc atgatctcga ccttgggctt
     1981 gccgagcgtg cccttcatcg cgtggatctg gcggttcacg ttcgtcacgc agatccgatc
     2041 gtagtcgcag aggatcacgc gaccgacgcc gctgcgcacg agcgcttccg ccgcgaacga
     2101 gccgacgccg ccgacaccga acacgctcac cgtcgaaccg gcaagccgag caattccctc
     2161 atcgccgagg agacgggcag cgcgatcgaa ccggcgatgc gtgggcatgc ggcgcattct
     2221 agggcggcca acgaaaaatg cgacgagcat tttccgtcgt cagccgggcc atctcctccg
     2281 gcgatgtctg acgcgcggcc gcgagtccac caatgacttc agccacgtac gcgggctcgc
     2341 tccgactgcc ttggtgggcc gcgggcgcct ggtccggcgc gtcggtctcc gcgagcagca
     2401 gctcgcccgg cacgacgcgt gctgcttcga gcggccgcct cgcattcggg tacgtcaccg
     2461 gcccggcgaa cgcgaacgcc atcccgaggt ctcgatagat cggcacgagc gccgcaccgc
     2521 ccgaatagct gtgcatcacg ccaccgacgt cgctcgcttt ctcctcgcgc agaatgcgcg
     2581 gcgcgacatc atgcgcacgc agcacgtgca ccaccagcgg cagcttcagc tcgcgagccg
     2641 cccgcacgtg cgcacgaaac agctcctcct gccgcgcgtg ctcgcccgtc ccaccatcga
     2701 gcccgcactc gccgaccgcg acggcgagcg ctccggcctc ccgcgtcgcc tcgatgatct
     2761 gcgccgcgag atcgccgact tcagcctccg cgagctccgg cacgatctga ggatgcacgc
     2821 caagcgcgac gcgcagcatc acgtgtgatc ggcccagctc gacgagcgcg cgccacgttg
     2881 ccggccggat cccgggcacc agaacgccgc gaacgcccgc agcctccgct cgcgcgatca
     2941 cctcggcgcg atcggcgtcg aagcgctcga ggtcgaggtg gcagtgcgtg tcgatcaacg
     3001 cacgcagtgt accgttacag gcgtgcggcg accttgcgcc agatcggcag ctcgcgcgtg
     3061 atgccggcag cggtcacggc cgcaacctcc ggctcgcgat cttcggcgac cgcgcacgcg
     3121 acgtggacga tcgcggcgag ccggcgcaag tgggtcggcg ctaggtctgg acgctcgtgg
     3181 cagcggatga gctggcgaat gaccgggccc acgtcggcag cgcgcggcgt gaccgcgagg
     3241 taggccgcga ggaacgcgat cgttcgatga cggtcgccgg caagtgcacg cgcgatgacg
     3301 gctgcacggg cagcgggtga accctcgacc tcgagcatgc gttcgtccag cacgttgatc
     3361 tggatcggcc gggtgcgggc gctttgtatg tgagacaggt aggatgcatg ggtggtccgt
     3421 aagaccctga agggctggtc tcactggtgg aggaccagcg agagcgtgct cgcgcgatgg
     3481 cttcgtgcgg cacacgaggc attgctcggc gagatgccga tcctggcggc aggtaccgcg
     3541 ctgttcgcga tcatcgccgt cgtacctaca ctcgccgcgg cggtcgcgct gttcggcctc
     3601 atggcggacc cgtcggagat cgcgggccag ctcacggcgc tgcgccatgt actgcccgtc
     3661 gaggtcgtcg acttcatcgc gaaccagctc acgcggcagg cgtcgcgagg caccacggag
     3721 ctagggctgc agctcggtat cagcacattc ctcgccgtgt tctccgcgcg tggctctgcg
     3781 cgtgcgctga tcgactcgct caatcgtgcg tatcgcgtgc gggagctacg ccggccgctc
     3841 gcccgcttcg cgctcacgct gctgatggca accgcgacgc tcgttggcct catcacgatg
     3901 ttcgcggtgc tcgtcgcatt gccaaccatc gtcggagcga tcaacaagca ttggatcgct
     3961 tgggcgatcg tgctgcgctg gccggcgctg ctcgcggtcg tgttcctgtc gctgctcgca
     4021 ctctatcgct acggtccatc gccgcggccc ctcggcccga aggagcgcat cgagacgccg
     4081 atcagcctgc cgccacatcc gatgcgccac gtctgcttgc ccggtgccgt aatcgccacg
     4141 gtgctgctct tgatcgtctc gtggggcctc tcgctctacg tcgacaacat cgcgaacacc
     4201 gagctcgtct acggtgcgtt cgggtcggtg atcgtcaccg tgctctggtt ctacttctcg
     4261 acgatggctc tgctcatcgg cgggttcgta aacgcagaac tggagcgtca ccgtggtgcg
     4321 ccccagcccg atcgcgagat gtattagcga ctagagacgc tggccggtga actggccgtg
     4381 cgcgagcggc gcgccgttac cgccgtagct gagtgtgcct gcccacgcgc cgacttggat
     4441 cgaatccatc ttgccgtcgt ggttcttgtc gatgcccttg gcgacgccgt tcacgttgaa
     4501 ctcgagcgcc gacgaatcga tcttgccgat ctgcgcgtag atgacctgcg agccaccgtg
     4561 cagacctgcg gtgcatgccg cttcgaacgt gctcggcgcg ccgatgccga cggcctcgta
     4621 cacgtactgg ccaacggcgt ggcagttgac caggcccgtg aggatgtctt cgaggttcgt
     4681 cgcgctcgga tcgacgagcg ggatgatgac ttcgtccatc gcgatgcgca tgaccttgcc
     4741 gtacgagagc ggaagcttgt ggtcggcgac ggtcagcttg ccgagcttgt cgacctgcac
     4801 gccgacgttc ggcacggcga cgtcctgcat ctggtagtcc ttgaacatgt actgcagctc
     4861 gatctgatcg accgtgaact gcacgccgtt catcgtgtgc gtcgccatgc cgttcgcggg
     4921 aacgtcgagc gtctcgagcg ttccgaagtg gcgcgcggcc tggccgaact tgttgcccat
     4981 gtcgcggatc gtcacgacga agtcgggcgc gacctgcagg aggcgatcgt tgaggtaacc
     5041 cgagacgaac gggatggcac ccttcacgat gttcttgaac gtgccgtcgg gcagcttcgc
     5101 gacgagctgg tcgaggatcc agtgcgccgg atcatcggca tcgtccgtcg cggcgatgaa
     5161 catgttgacg acttcgccgg cggtgcccgg catgttcgtc gcgatgtcga agtcgctgac
     5221 gagtgcgtac ttgccttcgg ccgtgagcgg cggctgctcg gcagagccag agccgcttcc
     5281 gctgccttcg cccgggtcat catcttcacc gggcgcttga gcagcgcagc cagcggcggt
     5341 cgcgcccagt gcgagcacga aggagaacgt cgagaacagg ctgcgcttca tcatggtgtg
     5401 cagccttggt gcagtgcgcg tgccgtgcgc gattcgcgat cgatcaagca cttcgcgatc
     5461 agactcttct ccccggcaat tcgtctggcc gggttcacag tccgatcctc ggacattcgg
     5521 agcgggctcc gaaaacgccg aaaaaaaacc gacccaggga ccattccctg ggccggccag
     5581 ccatcctcga cgcgcgggct caccacccgt gtgcgtcgaa ccgtttgtta catgcgctgc
     5641 gcggtgaagg tcgcggccgc gagcggggcg ggcgtacccg agtagctcag gttgccggtc
     5701 cacttgcccg tctgcagagc atcgaccttg tggtcagcgt tcgtgtcgac tgccttcgcc
     5761 gtgccgacga ggccgaactc gagagccgat gcatcgacgc cgtcgagctt gctgtagatc
     5821 gtctgggcgc cgtactggag gccgccggtg caggccgact gccagaacga agcgccgccg
     5881 ccgaagccga actgcgaggc gagagccgcg tcgatctgca cgccgactgc ttggcagttg
     5941 acgaggtccg cgaggagcgt ctggaggttc gtggcgttcg agtcgagcgc cgggatgatg
     6001 acctcgtcga ggccgatgcg gaggaccttg ccgtacgaga gcggcagctt gtgctcggcg
     6061 atgtcgagct tgccagtcag gtcgatcttc acgccgacgt tctgcgccgc gacgttcgcg
     6121 atctggtggt cagcgaagtt cacgtcggtc tcgagcgtgt cgatcttgaa gtgaacgccg
     6181 agcgccgtaa cggtcgagtt gtagtccgtg cccgactgag cgacttcgag ttgctcgttg
     6241 aggccgaagt tcttcgccat cacgccgaag tcgtggcccg tctgcaccat ggtgctgacg
     6301 aagtcaggag cgatcgtgag gaggcgctgg ttcaggtaac cggcgacgaa cggcttcgcg
     6361 ccagagagaa gattcttgaa cgtgccgttc ggcatctttg cgatgatctg gtcgaggatc
     6421 cactcggtcg gatcagccgg gccgtccgtc gccgcgatga tcgcgttcga gacttcgccg
     6481 accttgccgg gcatgttcgt cgcgatgtcg aacgtgctgc gcatttgata cttgccgctc
     6541 gcgtcgagag cgcgggggac gtcatcaccg ccaccgccac caccgccgcc gccaccacca
     6601 ccacccatat cgtcggggga gtcggcggcg cagccggtaa cagcggaacc aatggcgagg
     6661 gcgagtgcga ggctgagggt cttgacggtc ttcatttgaa atctcccggt cgaggattgc
     6721 tggttgatgg caaacccata tagcgaggga gatgccagat ccggccgatc cggaaaccgg
     6781 gggaagacgc gcacttgcga gccctccgag gaggaccggc aaattcgcta gccaggtcag
     6841 acgagctttc gtggagtcag ctgaccgggg cagaagccat tgcaaacgat cggttgcagg
     6901 ccgtcacctg cagatcgttt ggactcaaac taccacccgc actgatcgcc cttgttctgc
     6961 tcttcgagcc acttctgcag cggcgcgaag tactcgacca tcgcggtggc atccatctcg
     7021 cgctgaccgg tgagctcgaa caacgcgtcc tgccagggct tcgacgcgcc catcgcgagc
     7081 atcttcgcga acgcagcgcc ggcttccttg tttccataga tcgagcactc gtgcagcggg
     7141 cccgtccagc cagccttctt gcagagcgcg cgatggaact ggaactggag cacccgcgcg
     7201 aggaagtagc gcatgtacgg cgtgttgttc ggcacgtggt acttcgcgcc cgggtcgaag
     7261 agctccggct cggtgcgcgg cagcggcggg acaacgcctt ggtacttcag cttcagatcc
     7321 caccagtgct tgttgtactc gtccggcttc accttcccgc tgaacacgtc ccagcgccac
     7381 ttgtcgacaa gcaggccgaa cggcaggaac gcgatgctct cgagcgcctt gtgcatctgc
     7441 gcgttgatcg tcgccttgtc gttcttctcg accttgtcga gcaggccctt cgtgtggagg
     7501 tactcgggcg tcatcgagag cgcgatggtg tcgccgatcg cctcgtggaa gccgtcgttc
     7561 gcgccgctct gaaagaggac cggcagcttg tagtagttgt tgtagtagta gtcgtgaccg
     7621 agctcgtgat ggatcgtgac caggtcttcc tggttcttgt tgatacacat cttgatgcgc
     7681 aggtcattgt tgagcgtcac gtcccacgcg ctcgcgtggc agacgacctc tttgccggcc
     7741 ggcttcgtga actgcgactt ctgccagaac gactctggta gcgggttcat cccgagcgac
     7801 acgtagaacg cctcgcccat cttcaccatc ttgatgggat cgaacttctt ctcgagcgtc
     7861 gacgtgacat cgacctgcgc gacgcccttg tacggctcga gatcgctgta gaggtactcc
     7921 cagccctgcg cccacatgtt gccggtgagg tggctgaaga tcgggccgtt cttcggttgg
     7981 accttgtcgc cgtacttctt gttgaggcca cgccgcgtgt agcagtgcag ctgcgcatag
     8041 agcggcttga gctgctcgta caggcggtcg acttcctgct cgaacttttc ctcgggcatg
     8101 tcgtacgagg agcgccacat cgacgagacg tccttgaagc cgacgccacg tgcacccgca
     8161 ttcgcgagct cgacgtagcg cacgaacagc ggccgctccg cgcgacccac gttgtcgtgc
     8221 cagcccttcc aggcggcgag cagctccgcg ggcttcttgc tcttctgcag caccttcgac
     8281 agcgcgtcga gatccttgca gtacttgcct tcgactttcg acagcgcctt ctgcgctgcg
     8341 gccttgccct tgtcgtcttt cgccgagacg accttcgcgc ccgctgcgtc gagctccttc
     8401 tgatgcttcg agtcgcagac cttgcccttg ccatagatcg acgtcatctc ggcgccgagc
     8461 ttcgcgagct cttcggcctg cttcggatcg tcgggcgcag gctgccccga gaacttgagc
     8521 agcgtcagct ggcgacgcgt gtcggcgtcg agcttgccca tgatcggctc gaacttgcgc
     8581 gatgccttga tgagccgcgt gatgccattc gagaggtctt cgccggcttt cgccgcggca
     8641 gcttcgtgct ccggcgtgat gtccgtctgg tttgcccaat cagcgaccga cgaatcggtg
     8701 aacagctggc gcagctgctt gtcggcatcc gcgacgaatt gcttcgcttc agcgatcagc
     8761 ttcgcatcgt cgttgcccgc gggcggcggc ggttgcgtct gatccggtgg cggcgtctgt
     8821 gcggtgtcgc cggtcgtttg atccggcggt ggtgccggca ccggagcggg cttcgaaccg
     8881 ccgcaagcag cgaggaggag aatcgggaaa agcttggccg tacgcatcgc cgcggagcct
     8941 acacccgagg agcggcgttt caagcgcgcg caacgtgcgt tcacattgtg cgaccgctgc
     9001 gacagcctgg gacgtctacg gttccatgcc gggcagcggc gtaccgtcgt cgctcgagtc
     9061 cggcgcctgt tgcttctgct cgccgggaac gtcgacgccg cgatcctgca ggatgcgctt
     9121 ggccttctcg agctgcttgg catcgagcaa tgcgagcgcc ttcttcagcg catcgcgttc
     9181 gttgtcgtca tgcatcttgt ggaggcgctg cgagtccgcg gtctgcatgg tgctcttgcc
     9241 aggcgcgttg tcgtagcgct gccccttttc gccggccttc tgctgttgcg gcgagagctc
     9301 ctcggcgggc atcggctgct cgatctggcg aatctgcgcc tcgaggctgc cgttctgcgc
     9361 tgcgaggctg ttgtcgatct tcttcagctt ctcgagctgc tcgcccgaca gctgcaggtc
     9421 gcccgacgca tcgacgagat accccaccgc ggtgcccgac gcaggcggca agggaggcgg
     9481 cccacccggc tcgatgactt ccgacttctc tttgcacgct gagacgcccg caagcgcgag
     9541 cgcgatcgac agcacgaacc tcatggcgct agttatagcg tcattgcggc gcgagcaccc
     9601 gagcgctgcg agcctcgagc gtcagcggcg ccgtaaccgt cgcgcccgac agcagatccg
     9661 tatacgtgcc ggtgggcagg ttcgttgcct gctgcgaatt gtcgccgcgg ttgagcgcga
     9721 cgatcaccga gtcgcccggc atcgtcatct tatagacgag cacgtcttgc gagacaccga
     9781 gcgtctggcg tgagcccttg cgcatcgatg cgtgcgcgct acgcaacttc gcaagcgccg
     9841 cgatgcgatc gcgcagcatc gtctgattcg ccgtgtagtt gtcccactgc atgaagcggc
     9901 ggttgtccgg atcgcccgcg ccgggcatgc cgacctcatc gccgtaatag atcatcggga
     9961 tgccgggaat cgtcatgagc agcgtgtacg cgacgacaag gcgctggaac ggattcgcgc
    10021 tcgtcggcag cgacggctgg ccattccacg cgaggttctt gccgccgtcc cacgcgccga
    10081 acatcggatt gtcgagagcg agctcgatcg cgcgtggcac gtcgtggttg ccgatgaacg
    10141 tcgacatcac ggagccctgc ccgtagtagt tgtcattcgc gtcgaggaat ccggcgaggt
    10201 cgttcatcga gccatcgcgc cgcagcagcg tgccgaggat ctggccacgc agcgggaagt
    10261 cgaactgacc gtcgagcatc gtgttcgggt tgacgtacga cttgatgatt ccgcgatcgc
    10321 cgtcgaacgt ctcgccgacc atgtagaact tctggtcgaa tgcgacctcg gcattgagcc
    10381 gcgcgcgcac gtcggtcagc cacgacgtct cgatgtgctt caccgcatcg agccggaacc
    10441 catcgatgcc catgcgcttc gcccatgcga ccgcgttgtc gacggaccac ttgcgcgcat
    10501 ccgcattctg gaaattgaag tcgggcagga acggatcgaa ccagcacttc agccgatcgt
    10561 tgccccagtc gcagccgccg ccacacacgc agttgccgcc gaagccattg tcgtttggcc
    10621 agaaccactc gggatgctgc gcatagatcg gcgacgccga gtgcacgtgg ttcatcacgt
    10681 agtcgatgag gatctggatg ccgtgcgcgt gcgccgtatc gacgagcgcc ttcagctccg
    10741 cttcgctgcc gagcttactc tccggcttgc tcaagtcctt cggccagtag ccgtgatagc
    10801 cggagtagct gtggccgtcg gagccggggt tcgcgttgtc cgcgttatcg agcggcgacg
    10861 tgatccagat cgtgttgatg ccgaggtcgt tgaagtagcc ggcttcgatc ttctgcttca
    10921 ggccgacgaa gtcgccgcct tgatactgcc ccggctgctc gacgccggag atcggcgcgt
    10981 tgttcgtcga gtcgccatcg gcgaagcgat cgatcatcac gaagtacatg atcgcgtcgg
    11041 accactccat cgcaggacgc gcggggcact tgtcgcaatc cgcacgagcg acgctgttga
    11101 atgcgccgta gccatccggg ctcttgcgcg ggttctccgg gtcggcgatc cagttgccgt
    11161 cgacgacgag cttgtagacg atgatctgat tgtcggtgac gggcagcgtc gcttcgaacg
    11221 agccgtcggc gcccttcgtc atcgcgatgc cggtggtcca gccgtccgca gcgaagtcgc
    11281 cacggagcga caccgccgag gcattgccct tgaaccgaat cgtcgtcgtg caggtcggca
    11341 ccgcggcaca ggtctcgccc gagcctgcat ccatgcccgc atcgggagtc ccggtcagat
    11401 cagggtcctg cgacgaaggg cagccagccg cgaagacaac aaacgcagct gcaagggccc
    11461 aacgcatggc tgagcccgca tcaagtcgca tgccaggcgg ggtgtccagg acaatcgata
    11521 gttgctgcaa aacacgccgg attctcgttg ccgatcgcaa cgcgaaacgc caagccacgt
    11581 cgcgacttgc cgcagatcca gcgtcctgca ccgttgcgat tcgggcatgc attgtgctcg
    11641 attgccgggc ccatgcttcg gcgcggcagt ctcgtgatcg ttctcgcgct gacgagtcgc
    11701 gcgctcgcgc agggcaccga gatgccgcaa gaacccggcg ctggttccgg ttcaggctct
    11761 ggctctggct ctgcaacgac gtcggttccc gagcagccgc cgccgagcca gccgaccgcg
    11821 cccgatgact cggcgcccgc gcccgtaaag cccgagcgcg ccgacgaacc gccgcaggat
    11881 ctcctgaagc tgttcgggcc tacgccgcac gaagcgccga caagcgacag cggatttcgc
    11941 ttcggctcgt acggccgcgt cggcgtcggc accgatcttc gcggtggcaa gccggagaaa
    12001 atttcgctcg tcgcgcacgg cccgcgcatc gtcgagccga gctacctcga gctcgagctg
    12061 tcgtacggct tcgtccgcca gcatgcggac acgaccgagc cgatcatcct gcggccgatc
    12121 atcacgatcg cattcgacga cacgctgttt cacgagaccg gccagttcga cgcgcagccg
    12181 gcgctgcgca acatgtatct cgacgcgaag gtctacaatc acctgtcgct gtggtgcggc
    12241 tcgcgcatgt atcgcggcga cgatatctac ctgttcgact actggccgct cgacgatcag
    12301 aacctcgtcg gcgccggcgc cgaagtgacg cagctcaacg acctgagctt cgccgcagcg
    12361 gtcggtgcca atcgcctaga gcatccgttc cagcaccaag agatcgacgt cgcgaatccg
    12421 gtgcagggcg cgacgaccgt cgagcagctc aaccgccagc gcgtcgtcgt cagcggcagc
    12481 acgacgtata tccacatgcc cgagaacgca acgtggggcg cgaaggcgaa gctgcacgcc
    12541 gagatccact cgctgccgtc gggaacgcgc aagcgcgaag acggaacgtt cgaagcactg
    12601 ccgcacgaca cgggcgagct catcggcctc gaggttggcg gctttcagaa cgagcacgac
    12661 ggcctgcgcc gtcacctcaa cggcttcatc cgttacgcga agggcctcgc cgccttcgac
    12721 gagctcgcac cgccgacgac gttcgggccc gagctgaaga ccagcaacgc gagtgagctc
    12781 acgttcggcc tctccggcaa ctacgatcac gagctcggcc agctgatgct cggcgcgctg
    12841 tcgcgtcgct tcatcgatgc cgatgccgat gcgacggatg ccgacgacgg ctgggagtac
    12901 gcggtcgatg ttcgcccgct cgcgcgcgtc gttcgcgacg tctacgtcgg cgcggacatc
    12961 tcgtatcaag cgcgcttccc acgcggtctc aatccgatca cgctgcgcgc agaagatccg
    13021 gcgatgttcc agatcgcgcc gatgatcgcg ttttcgccga tgggaccgtc ggcgtacgac
    13081 cggccgcagc tgcgcctcgt ctatcgctac gcgcatctca acgacgccgc gctcgactcg
    13141 tacgttccgg atgatccgcg gcacgcgcac gcgacggtcc actatctcgg agtgcaagcc
    13201 gagtggtggt tcaactcgac gacgtatcgc tgactactgg cagctgtggc agagcgcggg
    13261 aacgctcgtg cagcccaagc cactgcggca tgcgagcgag ctgcagttga tgccttgctg
    13321 acagctctgg ctgcccgtgc agaggacatc acacgcgcac gagttgccgc acgcgacgtt
    13381 cttgcacgac tgcggtcccg agcaagccac cgcgcatggg cccgcgccgc actgcacgtt
    13441 ctcgcacgat gacctcgacg tgcattcgat gttgcacgac gcgcccatgg tgcagttgat
    13501 gccgttgcga cacgcgttgt cggtgttgca ggagatgtcg catttgtagc cagcggggca
    13561 gaccacggtg ttctggcagt tcgccgtcat gcagttgatc gagcaggtct tctgctggac
    13621 gttgcacgag ctgcacgctg cggggcagcc ttggttcgcg tcgccggggc gaggaccgtc
    13681 gaggcggggc gcatcgatct gcgagcccgc cacgacacag aagccgccgc tgcatacgcg
    13741 gccgctatcg cagtcggtgt tgagctcgca cgagtacgcg tcgctcttgt gattgatcga
    13801 gcacgcggcg agcaggaggc cgatgcaaac ggcgatgctt ctcatggcgc gcctccaaag
    13861 gtgagcacga tgccagcact gttcggcgtc agcgtcggcg tgatacgcgc cgtctgcgct
    13921 ctggctcgac ggtggccacg ccagtacgag tacgcgctca ccgtgccgag aaccacgccg
    13981 gtgaggaaga acagattgcc gccgctcgcg tagccgtccg catctttctc gagttgcccg
    14041 agcttcacga agtcggcggg cgtgttggcc ggcgactcgt cgatcagctg ctgcgtgtcg
    14101 gccgccttcg accacatcag gaagctcagc acgacgagcg tgccgccgac gcccattccg
    14161 atcttctgcc agcgatagct gcgctgcggc tggtcctgct gcatcggcgc gacttggccg
    14221 gccggcgcag cgctgacgct cgggtcgggc tgcggctgaa cgggcggcgc gtcataggtg
    14281 ggattgctcg gcggcggcgc agcctcgggc ggcggattgg cgggcgtgat cggaggcggc
    14341 gttttgtcgg gcgcgggcgt cgtcgaaacg gcagagacgc caaacagtgg accgaggtcc
    14401 tgcgtcagct tcgcatcgga tggcttgccc gcaggttgca ccgattgcgc ggcgcgcggc
    14461 gccgaaccct tggctagacg gcgcacggtg acgttcgtgc cggtcggtac cgaagtcgca
    14521 gtcgtcgcga cgatctcgtc gacaccaagc gtcgagagga cgtcgtcttt gcacgacgct
    14581 tcggacggat tgcagccgac gatagctgcg gcgtccgaga gcgtgacgtc gcccatctcg
    14641 actttaccgt cgaggttctt ggcgagcttg aggacgctcg cgtcgaggtt cgtgcgcgtg
    14701 ctcgcatccg ccgtgccctc ggctttgagc acgaggatcg ctttgggcgc agcgctcgcg
    14761 gtcgacgcga atgcgaacac gacgaggaca gctgcggcaa cgatacgctt catgagccga
    14821 gccgctccaa tctgcctttg atttgttcgg agtcgttcgc gcccggcgcg agctgcaggt
    14881 accgcttgta cgcagcgcgt gcttgctgcg tctcgcccat cttctcgaac acgagaccga
    14941 ggcctcgcca cgtcggcgcg tagttcggat tcgccgcgag cgacgtgcgc agtgacgcca
    15001 gtgcacccgc cgtatcaccg cgcgcgaact gctgcaggcc ttgccgatac gcgctctcga
    15061 catcgacctt cgcggtgttc ttcgtgtcgg tcttcgcggt cgtcttcgtg tcgaccttgg
    15121 gcgtcatctt cgtatcagcc gtcgcgacct tcgtgttcgt cttcgcggtc gtcgtcgttg
    15181 tcgggcgcgt gttttgcttc gcggcgcgct cggcggcgag cttctcggca gcggccttct
    15241 tgtcggcagc tgccttctct tgcgcggctt tgcgatcggc ggcgagcttg tccgcggccg
    15301 ccttcttgtc cgctgctttc tgctcggcgg cagccttctc ggccgcgcgc ttgtccgcga
    15361 gcgcctgcgc ggccgctgcc ttgtcggcag ccttcttgtc ggcggctgct tgttccgccg
    15421 ctttcttgtc gaggagcgcc ttgtccgcgg cagccttctt gtcggcagcc gcttgctccg
    15481 cggcgagctt ctcggcggcc ttcttgtccg cggcggtctg ctcggctgtc gccttctccg
    15541 cagcagcctg atccgccgca gctttctcgg cggcggcttt atcgtcggcg gctttctgcg
    15601 ccgcggcttg atcggaagcg gcttgatccg tcgcagcggt gttcgtcgcg gcagggtcga
    15661 cggcggagcc ggccgagccg gtatcggtcg ccggcagcgg ggttggcgtc ggctcggtcg
    15721 ccgcctgctg cgcgtcggca gtttgtgccg cggcgaccgg gcgattcgat ttcttcttcg
    15781 ggctcgagcc gccggcgacg agcgcgatga tcgtgacgag cgcagcgacg gcgacgccgc
    15841 caccgatcac gtacatcagc gtcttcttgt tatcgggcgg tgcgtgaaaa tcctcgtcgt
    15901 cgtgcgcgta gccctccggc gcgagcgcct cgtgcttgtc ggtgccccag ccccagcccg
    15961 cgagcacatc gcccttcgac tcgcgcggcg ccggtccgag cgaaacggcg gcgacgggat
    16021 gcgggcccgt tcgcttcgtc gccttcttgt cgtcgggctt cttctcctcg accttcgcgt
    16081 caggctgcgt atcccttacg tcggccttcg cgtcggggag tgtgtcgcga acgtcggctt
    16141 tcgcctcgac cttcacgacg ggcgcgacca caggcggagc ctcgagtggc ttcttcagct
    16201 cgacggtcgg cgtgccgttg gtttcgatct tctccgcggg cttcgcgggc tcgcgaatcg
    16261 tctgaccgag gcccggcgat gtggagacct tgtcgtcgac gatgacggcc ttcggtttgt
    16321 cctcgacgat caacgcgtgt ggcttgcttg gctcgtgctt ggcttcgacg actgcggcgc
    16381 cgttcgtcgc aactttcacc tgcggcggca cggagacaga gatcgtcgac ttgtcgttac
    16441 gaatcgatgt cgacgcgctc ggcaacacgg gcgccatcgg cacgagctcc gcgggtgctg
    16501 gcactggcgc aggcgcagcc gcgggcgtgg gcgccggcgc agcgggaaca ccgacggcgg
    16561 ttccgctcga cgctgcactt gcgagcttgg aggcgagtgc gacgcctggt gacgtgcgcg
    16621 gcatcgcctt gaggtactgc gcgatccgct cgtcgttgtc gtcgtagccg gcttcggaga
    16681 ggaactcctc gatctcgacg gccatcgcct tgaggctctg atagcgatcg gatggattgc
    16741 gggcgagcgc gcgcatgcag atcgccgaga gctccgcggg cacgttcgcg ttgagctcgg
    16801 tcggcggcgt gatatctcgc tcctgcacgg cagcgcgcac ggctgcgtcg ctcatcgcgt
    16861 cgaacaggcg ctgatacgtg agcacttccc agagcacggt gccgagcgag aacacgtcgc
    16921 tcttgcgatc gccaccgccg gtcagttgct ccggcgcgct gtagccgagc gagctcgtgt
    16981 ccgccggtga gaacgtgact tcaccggacg gcgcgaacgt gatcgcagtg ggcgtgatcg
    17041 ggccgaccgc tttgccggcg ccggcctttt gctgcgcggc atgaacttgc agtgcgacct
    17101 ttccgatcac acgcgcgctg cttagctcgt cgagtctctt actgctcttg ccggcgtcga
    17161 ggagctggcg gagcgtgtcg gctgctgcca cggccacatc gtaacgtagg ctcgagcgaa
    17221 acgcccctgt tccaaccctg cacaatgctt ggcggctata cctacatccc gggccatgat
    17281 catgagtact tcgacgctga ctcgccgtgc gtttctgggc gcggagctgc cggccgatgc
    17341 cgacgcattt acacccgatg gaatgcgcgt ggccggtgtc gtggagaaca gcatggcgat
    17401 gcgcgcgggg ctccttccgg gcgatatcgt gacttcgatc gcgaaacagc cgacgcgcaa
    17461 tttgtgcgag ctgtcccatg cgctgcgcgt cgc
//
LOCUS       DATLBZ010000217        13066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_218937, whole genome shotgun sequence.
ACCESSION   DATLBZ010000217 DATLBZ010000000
VERSION     DATLBZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13066
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218937"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2028
                     /locus_tag="VMZ53_11560"
     CDS             <1..2028
                     /locus_tag="VMZ53_11560"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HUS29145.1"
                     /translation="KKGKPDEGVPGITLGAFSIAAKAFGLAIEPSGPDGSFEIYGVKP
                     AAYMLFAVGEGTVPEIGKNVEVVDKDVTGVIVEMTAGVTVSGVVSPPLAGALITVELE
                     GDFNMARMFEMAKLVLIRGETDQAGAFTLKNVPPGAFKLNAGAPDGHTGEQPVVVADT
                     DKAGIVIKLEPRASVSGRVTDSNGQPVAGGRVNVDPLDPDADKKMNISFNDMRNRNSA
                     TTGPDGTYKVVGLEAGKVRVRASDSMGESVYYMAKKDQPSKSAVEITLVAAQNTPNVN
                     LQVEARDGTIKGTVLLPDGKPAQDAWVTAQRAREKSVGDKEVVFETWYGPSAPPVLTD
                     SAGAFTIGKLRKGTYNLIVDGPKGTSHAESNGIKTGESVTIKLLALGTLSGVVTLAGA
                     PVPSYDIECRGPHFDNSEKHVDDKTGAYTLERLTPGEYTCSVDSPTGSGSGKVTVPPG
                     EAKLDITLTRFASVTGIVVNVLDKKPLPNVVVFAGNDANMNSATFSQMLSGTAPKTDA
                     TGHFKVNRVPTGKGKVAVMPKDGFMPLGTADYTVAEGQSVDVGTIAVVPPREGDAGTF
                     GMSVAPFGATPPGSASGSGSGAGAGSGSGFEGDRLAVLSVKDGGPAALAGIKEGDQIT
                     KINGQDVAKLTPFVASQLLASGTVGVGVTVALTIERAGAPAFAASITSIKW"
     gene            2068..2859
                     /locus_tag="VMZ53_11565"
     CDS             2068..2859
                     /locus_tag="VMZ53_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29146.1"
                     /translation="MRQFATIAMFATLALASLDATACPLDEGGTMLFPGGRSAFTGEV
                     LPLPSQPSLYYVEHYDGDDPTIEAESNGQPVPFTIVDISVDGSGASKRIDFAIESGPL
                     VVHSNSYRSFTFHYRVVPGYTPSHAATLELGPFQYGMIEYADIESEAGLYRIDRAGYS
                     GPSDQRAYTEDIYPRSGDFRVTAFFSDRTTAVIYDRDAALVGVIVDTARFARAWLPSF
                     ERQPEPPPPTWPQWLALALLLVACSLVRPRGSVLGAVEQNGQHDE"
     gene            complement(2815..3081)
                     /locus_tag="VMZ53_11570"
     CDS             complement(2815..3081)
                     /locus_tag="VMZ53_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29147.1"
                     /translation="MTRAAVLSLLLSAGCITNAQGRVNVTASTGTLGGALILGGAIVG
                     AGECQPTPDQCDHVERGEPAVAATLVLVGASLVVLAVLFDRAEN"
     gene            complement(3277..3801)
                     /locus_tag="VMZ53_11575"
     CDS             complement(3277..3801)
                     /locus_tag="VMZ53_11575"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUS29148.1"
                     /translation="MRSLVVAVFLLAGLLTAQVAHADALKVGDRLAELDVAVDGAGKS
                     FKLKALKGGWVLVTVGADWCKPCAKELPAWDKLAPEFKSKVKFVAINIADNIDDGKKF
                     NAKLKLKNLQLVYMPADKSAVVAKYGSDNMPTTFIADPQGVIRYVRAGFEKGDVSGET
                     KKMREQLTKLTAAK"
     gene            complement(3824..4729)
                     /locus_tag="VMZ53_11580"
     CDS             complement(3824..4729)
                     /locus_tag="VMZ53_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUS29149.1"
                     /translation="MTKGFLALNLAVLVALAAGCEKKPAKNGDTGAINAMDRAGSNDG
                     PVDTAPLDGIDISKLNKDKQATFYKLVGSLSSPCGTGKSLRTSYASEPSCKRAPFAVR
                     YVAALLEDEATEAQVKEEYEHKYESDAKPVKIDVSKAPRVGNDDAPIRLVEFFDYQCP
                     HCQAFKPMLEQVMADRPGKVVVHYMMFPLEKHTNSKLAAQAALAAAQMGKFHEMHDVL
                     FAKSPNHSKDDITGYAKEIGLDPAQFSSLLDAQGAHVVTDVAQGDAAGVDSTPTLYFN
                     ERKYEGPIHPKYIEMWIDEELAVNR"
     gene            4815..5462
                     /locus_tag="VMZ53_11585"
     CDS             4815..5462
                     /locus_tag="VMZ53_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS29150.1"
                     /translation="MTVHSAQDAVDKRDKRERILAAAERIFARHGFFAAKVSDVAKEA
                     GVADGTIYLYFKNKDDLLISLFERRMQQVNEALRAATAKVKSPREQLRAFVKTYLQLV
                     ADEPTAAEVLTIELRQSSKFMKEYENPEFADFLRLLGGIISTAQDKGEIESSIPAHVA
                     ARMIFGMLDELALAWVLAKQPLPAASGARPKKFDIVRAADWVITLVTRGLEKESL"
     gene            5459..6277
                     /locus_tag="VMZ53_11590"
     CDS             5459..6277
                     /locus_tag="VMZ53_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HUS29151.1"
                     /translation="MKILVPVKRVPDPTQKAKIKDGHIDLTGASWIVNTFDEYAVETA
                     LRLLENGESNSRTGGEIVVVSIGPDDAAKEIRTCLAMGADRAIHVVANDAEIDSLAVV
                     KVLQGVIAAEKPDLVVMGKQAVDGDANQVGQMLAGKLGWPQATFAASIEKQGDALVVG
                     REVDGGVEYKKVPTPAVITVDLRIVASKAVKNGVTPDSFSYSGEGARLPSLKGIMAAK
                     KKPMEAKKLADYGVEPQPKIKELSVMLPPPRPPGQIVADIPTLVKKLAEEAKVL"
     gene            6277..7245
                     /locus_tag="VMZ53_11595"
     CDS             6277..7245
                     /locus_tag="VMZ53_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HUS29152.1"
                     /translation="MGNVLAFCEFSGDALRTSALANIAFARTAAQAHGGEVIALLVGA
                     GAAGAAANAAKFAPKVITVEDGKLGNYLAETYAPVLAHLAKENNATVVTATATSVGKD
                     VMPRVAALLDAGMASDIAKADGANKFVRPILAGNAYSTVEITTPIICVTVRQSEFEPA
                     AELGSAGAVSSAPAGDVDAKGAEFVRLDAQKSDRPDLGDAKVVVSGGRGMKNGENFKV
                     LEQLADLLGAAMGASRAATDAGMVPADWQVGQTGKIVAPNLYFAVAISGAIQHLAGMK
                     GSKTIVAINKNKDEPIFQVADYGVVADWEKAVPELISEIQKLKAKG"
     gene            complement(7211..7681)
                     /locus_tag="VMZ53_11600"
     CDS             complement(7211..7681)
                     /locus_tag="VMZ53_11600"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HUS29153.1"
                     /translation="MPDASARSRRLGLGLAIMTIGIAVVVTQWRFHFDATRFGMHVRW
                     NRIDWHWFPRTPMGHVRLDKDFALNLLMLVPLGIGFALWRRASGLRIVIEALLLGFAT
                     SALLELAQLANAYRYTTFADVWRNTLGCVVGAIVIVALRRSAKVSPSPSASGSR"
     gene            7751..8998
                     /locus_tag="VMZ53_11605"
     CDS             7751..8998
                     /locus_tag="VMZ53_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29154.1"
                     /translation="MSFVVAVRLAAATLLLAACSFKHGALGGGGGDERVDAAIDPTDP
                     DATMSGGMDTMMTMPPPFMPLHVPTAQPGTGALTLSGTLTIDTDNLTYGPTVNGVAFA
                     TSPQTTGPTVAVLRVSTFTVSAGATISVRGARPLIVVASGTIQIDGILDAGGHGTTPG
                     AGGSASAAGAAKGGNGAHSGTYLDSGGGGGGYGAPGGAGGSASGLGGGGAGGTTGDAT
                     LTSLAGGSGGGNPAVLSCGTRYGGAGGGAIQLSTPTTIAIGANGRVLAGGGGGQGGTN
                     CNARNGNWGGGGGGGSGGAIVLQASTVTNTGRVAANGGGGGGGAGDPTGDGGAEGTVG
                     GPGEDGRSDTTQADGGDDVGSWSAAGGKGGALAGGAQDGVDENTGNGNAGGAGGGVGR
                     IVVSATTKNGAGTYSPAAGSVPF"
     gene            9040..9846
                     /locus_tag="VMZ53_11610"
     CDS             9040..9846
                     /locus_tag="VMZ53_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29155.1"
                     /translation="MRFIALGCLLAGCGRVGFESSRVAQSDATSSSPLDAITDTLAPL
                     SVCPFSANAPDPLTVSGITFQYTGPSNMTGATPSILVTASRGGTQIAQTTSDAAGDYT
                     LVIPTGGASTPIEIDYGPSGYWTTQVIRNVALDTNVTGPNTMFWRIGDGPIWGDGQMG
                     AVYAAASLTYDLAKATLQIAARDCAGAPIGGVTVTVEPPPEKLLYINSNGDVDGSLTT
                     SDAMYGHAVGFNAVAGPTHITASKDGVSIYEMDVDVQAGRYNVYPVFYIP"
     gene            9843..10631
                     /locus_tag="VMZ53_11615"
     CDS             9843..10631
                     /locus_tag="VMZ53_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29156.1"
                     /translation="MRICCAAVLLSGCGRLGFDARVASDAPDATDAAQPVCPVQSVPS
                     TITVSGDTFRYTTFTNMTAPIAGATVQARDRTDLSILATVTSDGSGAYSLSYSTDGDV
                     TPLALIYAAPTYNETIVIPSVVDRDIMGMTVAVFSVGDGPLWNDGALDSVYSAYGSGL
                     PRDATLGTINVAVRDCDGNPLEGASVTFDRTPDDFAYQRPDGAFDLSLTATVQPYTHL
                     VAFNMPLDSYRVSAQRPGYRFTDESIFTDASATTLVVIRGALAP"
     gene            complement(10620..10997)
                     /locus_tag="VMZ53_11620"
     CDS             complement(10620..10997)
                     /locus_tag="VMZ53_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29157.1"
                     /translation="MRFLLAALLVLTACSEKEDKLSTARRQADEAKATAVKAEADAAE
                     AQRKLLEALAEEKAALEDKASLEEQLAEAKQQTRELIEMANKKIAALKKQRDALPDGP
                     DRKALDTQIDELAKVVQDAQSGR"
     gene            complement(11007..>13066)
                     /locus_tag="VMZ53_11625"
     CDS             complement(11007..>13066)
                     /locus_tag="VMZ53_11625"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HUS29158.1"
                     /translation="ETIYWNANVSTNADGTASVSFPVSDAVTSFRATAEGVSAGGLPG
                     AGDVVIQSKMPLSLDAHLPVEVTSGDKIKLPVTLTNETDEEMDADLTAAFGSAFKLGA
                     NPSGKIHLKAHEKKSLFFPLDVVATTGEADVSLGVTARGLQDKLDKKIRVVPLGFPFE
                     LAASGTAKTGTPVKHDFELADALPGSIEATVTMYPSPVASMTQGMEAMIREPGGCFEQ
                     TSATNYPNIMILSYLATNDAADAQLVQKTQGHLDRGYKLLTGYETKQKGFEWFGQTPG
                     HEALTAYGLMEFEDMGKVYEVDHSMVERTADWLMSRRDGKGGFLRSSQALDNFGRANE
                     QTTNAYIMWALSEAHRTKGLDAELTAQKKLGEGTRDPYLLALVANTATLAKMPEADSY
                     LKKLVGLQDKDGSFQGAKESITMSGGESLTIETTALATLAMIKASPNNEYETQIRASV
                     DWLNKKRGGFGEWSNTQATILGLKAMTAYSDYAKQMASSGDATLMINGEPAGSIHFEK
                     GRKDALVWDDLEGKLKPGHNTIELSLAGGATLPYSIAVTYRAKNPASSADAKVNVTTQ
                     LAKEKVKLGEGVKLRARIENKTAEGLPMTLARIGIPGGLTFQTWQLKELRDKGVIDFY
                     ETRPREVIVYFRALAPNAKKDVELDLLAQTPGSYEAPASTTYLYYTAEDKTWVAPVKV
                     AIE"
BASE COUNT         2240 a         4273 c         4282 g         2271 t
ORIGIN      
        1 aagaagggca agcccgacga aggcgtccct ggcatcacgc tcggcgcatt ctcgatcgcc
       61 gcgaaggcgt tcggtctcgc gatcgagccg agcggccccg atggctcgtt cgagatctac
      121 ggcgtgaagc ccgccgcgta catgctgttc gccgtcggcg aaggcacggt tccggagatc
      181 ggcaagaacg tcgaggtcgt cgacaaggac gttaccggcg tgatcgtcga gatgaccgcc
      241 ggcgtcaccg tgtcgggcgt cgtctcgccg ccgctggccg gtgcgctgat caccgtcgag
      301 ctcgagggcg acttcaacat ggcgcgcatg ttcgagatgg cgaagctcgt gctgatccgc
      361 ggcgagaccg atcaagcagg cgcgttcacg ctgaagaacg tgccgccggg cgcattcaag
      421 ctcaacgccg gtgcacccga cggccacacc ggcgagcagc ccgtcgtcgt cgccgacacg
      481 gacaaggcag gcatcgtgat caagctcgaa ccgcgcgcgt cggtcagcgg ccgcgtcacc
      541 gattcgaacg gccagcccgt cgcaggcggc cgcgttaacg tcgatccgct cgatcccgat
      601 gcggacaaga agatgaacat ctcgttcaac gatatgcgca accgcaacag cgcgaccacc
      661 ggtcccgacg gcacgtacaa agtcgtcggt ctcgaagccg gcaaggtccg cgtgcgcgcg
      721 agcgacagca tgggcgagag cgtctactac atggcgaaga aggatcagcc atccaagtcc
      781 gccgtcgaga tcacgctggt cgccgcgcag aacacgccga acgtgaacct gcaggtcgag
      841 gcacgcgacg ggacgatcaa aggcaccgtg ctgctacctg acggcaagcc tgcgcaggac
      901 gcgtgggtta ccgcgcaacg cgcgcgggag aaatcggtag gcgacaaaga ggtcgtgttc
      961 gaaacttggt acggcccatc ggcgccgccc gtgctcaccg atagcgcagg cgcgttcacg
     1021 atcggcaagc tccgcaaagg tacgtacaac ctgatcgtcg acggccccaa gggcacgagc
     1081 cacgccgaat cgaacggcat caagacgggc gagagcgtca cgatcaagct gctcgcgctc
     1141 ggcacactct cgggcgtcgt cacgctcgca ggtgcgccag tcccgagcta cgacatcgag
     1201 tgtcgcggcc cgcacttcga caacagcgag aagcacgtcg atgacaagac cggcgcgtac
     1261 acgctcgagc gcctgacgcc cggcgagtac acatgctccg tcgattctcc gacgggcagc
     1321 ggctccggca aggtcacagt cccacccggc gaggccaagc tcgacatcac gctcacgcgc
     1381 ttcgcgtcgg tgaccggcat cgtcgtgaac gtcctcgaca agaagccgct gccaaacgtc
     1441 gtcgtgttcg caggcaacga cgcgaacatg aacagcgcga ccttcagcca gatgctcagc
     1501 ggcaccgcgc caaagacaga cgcgaccggc cacttcaaag tcaatcgcgt tccaacgggc
     1561 aaaggcaaag tcgcggtgat gccgaaggac ggcttcatgc cgctcggcac agccgactac
     1621 accgtcgcgg aaggccagtc cgtcgatgtc ggcacgatcg cggtcgttcc gccgcgcgaa
     1681 ggcgatgcag gcacgttcgg catgagcgtg gccccgttcg gcgcgacgcc accgggcagc
     1741 gcctcgggct ccggctccgg cgcaggcgca ggctcgggct cgggcttcga aggcgaccgc
     1801 ctcgccgtgc tctcggtcaa agacggcggc cccgccgcgc tcgccggcat caaagagggc
     1861 gatcagatca cgaagatcaa cggccaggac gtcgcgaagc tgacaccgtt cgtcgcctcg
     1921 cagctgctcg cctcgggcac tgtcggtgtc ggcgtcaccg tcgcactgac gatcgaacgc
     1981 gccggcgcgc ccgcgttcgc cgcgtcgatc acatcgatca agtggtgagc tagcgcaacg
     2041 ccagcccgtt cggcgtgtct tgcgttcgtg aggcaattcg ccacgatcgc gatgttcgct
     2101 acgctcgcgc tcgcatcact cgatgcgacg gcctgccctc tcgatgaagg cggcacgatg
     2161 ctgtttcccg gaggacgctc ggcgttcacc ggcgaggtcc tgccgctgcc gtcgcagcca
     2221 tcgctgtact acgtggagca ttacgacggc gacgatccga cgatcgaagc ggagagcaac
     2281 gggcagccgg ttccgttcac gattgtcgat atctctgtcg atggcagcgg cgcgtcgaag
     2341 cgcatcgact tcgccatcga gagcggcccg ctcgtcgttc actcgaacag ctatcgctcg
     2401 ttcacgtttc actatcgggt cgtgcccggc tacacgccga gccacgccgc gacgctcgag
     2461 ctcggcccgt ttcagtacgg catgatcgag tacgccgaca tcgagagcga ggccggcctc
     2521 tatcgcatcg accgcgccgg ctattcggga ccgtccgatc agcgcgcgta caccgaggac
     2581 atctatccgc ggtcgggcga ctttcgagtg acggcgttct tctcggatcg aacgaccgcc
     2641 gtgatctacg accgcgatgc ggcgcttgtc ggcgtcatcg tcgacaccgc tcgcttcgct
     2701 cgcgcatggc tgccgagctt cgagcgccaa cccgagccgc caccaccgac ctggccgcag
     2761 tggctcgcgc tagcgctgct cctcgtggct tgctcgctcg taaggccacg aggatcagtt
     2821 ctcggcgcgg tcgaacagaa cggccagcac gacgagtgat gcgccgacga gcacgagcgt
     2881 cgctgcgacg gctggctcgc cgcgttcgac gtggtcgcat tggtcgggcg ttggctggca
     2941 ctcgcctgcg ccgacgattg cgccgccgag gatcagggcg ccgccgagcg tgccagtcga
     3001 tgcggtgacg ttgacgcgac cttgcgcgtt cgtgatgcag ccggcgctaa gcaggagcga
     3061 gaggacggcg gcacgggtca ccgcacgacg ttttgcaacg cacatgcctc gcgcttggtt
     3121 gcgagatccg gtcgacggct cagccgcagc gcgggcgctg cgtatcgaag tgacgacagg
     3181 ggtggcgcac gggaatcacg gtcgcgatgg cgggaatagt cggcttgcag tccgcgtttg
     3241 cgcgtccgct ggccagctca ttgcgacggg gcggcactac ttcgcggcgg tcagcttcgt
     3301 cagctgctcg cgcatcttct tcgtctcgcc cgacacgtcg cccttctcga atccggcgcg
     3361 cacgtagcgg atgacgccct gcgggtctgc gatgaacgtc gtcggcatat tgtccgagcc
     3421 atacttcgcg acgaccgcgc tcttgtccgc gggcatgtag acgagctgca agttcttcag
     3481 cttgagcttc gcgttgaact tcttgccgtc gtcgatgttg tccgcgatgt tgatcgcgac
     3541 gaacttcacc ttgctcttga actcgggcgc gagcttgtcc cacgcgggca gctccttcgc
     3601 gcacggcttg caccagtcgg cgccgaccgt gacgagcacc cagccgcctt tgagagcttt
     3661 gagcttgaac gacttgccgg caccatcgac ggcgacgtcg agctcggcga ggcgatcacc
     3721 gactttgagg gcgtcggcgt gggcgacctg tgcggtcaga agcccggcta ggagaaagac
     3781 ggcaactacg agcgatcgca tggggtttgg acgggctccg gggctatcga ttcactgcca
     3841 gctcttcgtc gatccacatc tcgatgtact tgggatgaat gggtccttcg tacttgcgct
     3901 cgttgaagta cagcgtcggc gtggagtcga ctcccgccgc atcgccctgc gccacatcgg
     3961 tgactacatg ggcgccttgg gcgtcgagca gcgaggagaa ctgggccggg tctaggccga
     4021 tctctttggc gtagcccgtg atgtcgtcct tggagtggtt cggcgacttc gcgaacagca
     4081 cgtcgtgcat ctcgtggaac ttgcccatct gcgcggcggc gagcgcggcc tgcgcggcga
     4141 gcttggagtt cgtgtgcttc tcgagcggga acatcatgta gtggacgacg accttgcctg
     4201 ggcgatcggc catcacttgc tcgagcatcg gcttgaacgc ttggcagtgc gggcactggt
     4261 agtcgaagaa ctcgacgagg cgaatcggcg catcgtcgtt gccgacgcgc ggtgccttgc
     4321 tgacgtcgat cttcactggc ttcgcatcgc tctcgtactt gtgctcgtac tcctctttga
     4381 cttgagcctc ggtggcttcg tcctcgagga gcgcggcgac gtagcgaacc gcaaacggcg
     4441 cgcgcttgca cgatggctcc gaggcgtacg aggtgcgcag gctcttgccg gtgccgcacg
     4501 gcgagctcag cgagccgacg agcttgtaga acgtcgcttg cttgtccttg ttgagcttgc
     4561 tgatgtcgat gccatcgagc ggcgcggtgt cgacggggcc gtcgttggag cccgcgcgat
     4621 ccatcgcgtt gatggcaccg gtgtcgccgt tcttggccgg cttcttttca cagcctgccg
     4681 ccagcgcgac cagcacggcg aggttcagcg cgaggaatcc ctttgtcact gccaggttgt
     4741 agtgcggccg tgccctcgaa tcaactcggg ggaacccttg acggagtgcg tcacgagcgc
     4801 tatcaagcga ctgaatgacg gttcattcag cgcaggacgc cgtcgacaag cgcgataagc
     4861 gcgaacgcat cctcgctgcg gcagagcgga tcttcgcccg ccacgggttc ttcgccgcaa
     4921 aagtctcgga cgtcgccaag gaagcgggcg tcgccgacgg gacgatttac ctctacttca
     4981 agaacaagga cgacctgctg atctcgctgt tcgagcggcg catgcagcag gtgaacgagg
     5041 cgctgcgggc tgcaaccgcc aaggtgaaga gcccgcgcga gcagctccgc gcgttcgtga
     5101 agacatatct gcagctcgtt gccgacgagc cgaccgcggc tgaagtgctg acgatcgagc
     5161 tccgccagtc gagcaagttc atgaaggagt acgagaaccc cgagttcgcg gacttcctac
     5221 gcctgttggg cggcatcatc tccacggcgc aagacaaggg tgagatcgag agctcgatcc
     5281 ccgctcacgt cgccgctcga atgatcttcg gcatgctcga cgagctcgca ctcgcatggg
     5341 tccttgccaa acaaccttta cctgccgcgt ctggtgcgcg cccgaagaag ttcgatatcg
     5401 tgcgcgccgc tgattgggtc atcactctcg tcacgagagg cctggagaag gagtcgctat
     5461 gaagatcctc gttcccgtga agcgggtccc cgacccgacg cagaaagcca agatcaagga
     5521 cggtcacatc gacctgaccg gtgcttcttg gatcgtcaac acgttcgacg agtacgccgt
     5581 cgaaaccgca ctgcgcttgc tggaaaacgg cgagagcaac tcacgcacgg gcggcgagat
     5641 cgtcgtcgtg tcgatcggcc cggatgacgc tgcgaaagag attcgcacgt gtctcgcgat
     5701 gggcgcggat cgcgcgatcc acgtcgtcgc caacgacgcc gagatcgatt cgctcgcggt
     5761 cgtcaaagtc ctccaaggcg tgatcgcggc ggagaagccg gacctcgtcg tgatgggcaa
     5821 gcaagccgtc gacggcgacg cgaaccaagt gggccagatg ctcgccggta agctcggctg
     5881 gccgcaggcg acgttcgctg catcgatcga gaagcaaggc gatgcgctcg tcgtcggtcg
     5941 cgaagtcgac ggcggcgtcg agtacaagaa agtcccgacg ccggccgtga tcacggtcga
     6001 cctccgcatc gtcgcgtcga aagccgtcaa gaacggcgtc acgcccgatt cgttcagcta
     6061 ctcgggtgaa ggcgctcgcc tcccctcgct gaagggaatc atggctgcga agaagaagcc
     6121 gatggaggcg aagaagctcg ccgactacgg cgtcgagccg cagccgaaga tcaaagagct
     6181 gtccgtgatg ctgccgcccc cgcggccgcc cggtcagatc gtcgctgaca tccccacgct
     6241 ggtgaagaag ctcgccgaag aagcgaaggt gctctaatgg gtaacgtcct cgcattctgt
     6301 gaattctccg gtgatgcgct ccgcacgagc gcgctcgcca acatcgcgtt cgctcgcacg
     6361 gcggcgcagg ctcacggcgg cgaagtcatc gcgctcctcg tcggcgcggg tgctgctggc
     6421 gccgccgcaa acgcggcgaa gttcgcgccg aaggtcatca cggtcgaaga cggcaagctc
     6481 ggcaactacc tcgccgagac gtacgcgcct gtgctcgcgc acctcgcgaa agagaacaac
     6541 gcgacggtcg tcaccgccac ggcaacgtcc gtcggcaaag acgtgatgcc tcgcgttgct
     6601 gcgctgctcg atgccggcat ggcgtcggat atcgcgaagg cggatggcgc gaacaagttc
     6661 gtgcgtccga tcctcgcggg caacgcgtac tcgacggtcg aaatcacgac gccgatcatc
     6721 tgcgtcaccg ttcgtcagtc ggagttcgag cccgcagccg agctcggctc cgccggcgcg
     6781 gtgtcgtctg ctcctgccgg cgatgtcgat gcaaagggcg cggagttcgt tcgtctcgac
     6841 gcgcaaaaga gcgaccgtcc ggatctcggc gacgcgaagg tcgtcgtctc cggtggccgc
     6901 ggcatgaaga acggcgagaa cttcaaggtg ctcgagcagc tcgccgactt gctcggtgca
     6961 gcgatgggcg catcgcgcgc agcaaccgat gccggcatgg tccccgccga ttggcaggtc
     7021 ggccagaccg gcaagatcgt cgcgccgaac ctctacttcg ccgtcgcgat ctccggtgcc
     7081 atccagcacc tcgccggtat gaagggctcg aagacgatcg tcgcgatcaa caagaacaag
     7141 gacgagccga tcttccaggt cgccgactac ggtgtggtcg ctgactggga gaaggccgtg
     7201 cccgagctga tcagcgagat ccagaagctg aaggcgaagg gctaaccttc gcggagcggc
     7261 ggagcgcgac gatcacgatc gcgcccacca cgcatccgag tgtgttgcgc cacacgtccg
     7321 caaacgtcgt gtagcggtat gcattcgcga gctgcgcgag ctcgagtagc gcgctcgtcg
     7381 caaagccgag cagcagcgct tcgatcacga tgcgcagccc gctcgcgcgt cgccacagcg
     7441 cgaagccgat gccgagcggc acgagcatga gcagattgag cgcgaagtct ttgtcgagcc
     7501 gcacgtggcc catcggcgtg cgcggaaacc agtgccagtc gatgcggttc cagcgaacgt
     7561 gcatgccgaa gcgcgtcgcg tcgaagtgaa accgccactg cgtgacgacg accgcgatgc
     7621 cgatcgtcat gatcgcgagg ccaaggccca ggcgacgaga acgagcggat gcatccggca
     7681 tcatgggcat tttagcggtt aacccagtgt tcatattcag gggtcggccc tgtcggacgg
     7741 ctcgggtatc atgagctttg tggtagccgt gcgccttgcc gctgcgacac tcctactagc
     7801 cgcgtgcagc ttcaagcacg gcgcgctggg cggtggcggc ggtgacgagc gagtcgacgc
     7861 ggctatcgat ccaacggacc cggacgcgac gatgtcgggc ggcatggaca ccatgatgac
     7921 gatgccgccg ccgttcatgc cactgcatgt tccgacggcg caacccggca ccggtgcgct
     7981 gacgctgagc ggcacgctga cgatcgacac cgacaacctg acctatgggc cgacggtgaa
     8041 cggggtggcg ttcgcgacgt caccgcagac gactggaccg acggtcgccg tgctgcgcgt
     8101 gtcgacgttc acggtttcgg cgggcgcgac gatcagcgtg cgcggtgcgc gtccattgat
     8161 cgtcgtcgcg agcggcacga tccagatcga cggcatcctc gatgcgggcg gtcacggcac
     8221 gacgccgggc gccggcggaa gcgcatccgc tgcaggcgct gcgaaaggcg gcaacggcgc
     8281 gcactcgggc acgtatctcg actccggcgg cggcggcggt gggtacggcg cgcccggtgg
     8341 tgccggcggt tcggcgagcg gcctcggcgg cggcggtgcc ggcggcacga caggcgatgc
     8401 aacgctcacc tcgctcgcgg gcggatcagg cggcggcaat cccgcagtgc tctcatgcgg
     8461 cacgcgctac ggcggcgcag gcggcggtgc gatccagctg agcacgccga cgacgatcgc
     8521 gatcggcgca aacggccgcg tgctcgcagg cggtggcggc ggacaaggcg gcacgaactg
     8581 caacgcgcgc aacgggaact ggggcggcgg cggtggcggc ggatccggcg gtgcgatcgt
     8641 gctgcaagcg tcgacggtga cgaacaccgg ccgtgtcgct gcgaacggcg gcggtggtgg
     8701 tggcggcgcg ggcgatccca caggcgatgg cggcgcggaa ggcaccgtcg gtggtccggg
     8761 cgaggacggc cgatcggata cgacgcaagc ggatggcggc gacgacgtcg gctcgtggag
     8821 cgcagccgga ggcaagggcg gcgcactcgc aggaggcgca caagacggcg tcgacgagaa
     8881 caccggcaac ggcaacgcgg gaggcgccgg tggtggcgtc ggccgcattg tcgtatctgc
     8941 gacgacgaag aacggtgcgg gcacctacag ccctgccgcc ggatccgtac cgttctgagg
     9001 caattcgccc acacggcata tccccgctac gatcgtcagg tgcggttcat cgcgctcggc
     9061 tgcctgcttg ccggctgcgg tcgcgtcggg ttcgaatcgt cgcgcgtggc acaaagcgat
     9121 gcgacgtcat cgagccctct cgacgcgatc accgacacgc tggcgccgtt gtctgtgtgc
     9181 cccttttcag cgaacgcgcc tgatccgctg accgtttcgg gcatcacgtt tcagtacacg
     9241 ggcccatcga atatgacggg tgcaacgcca agcattctgg tcaccgcgag tcgtggcggc
     9301 acgcagatcg cgcagacaac aagcgatgcg gcgggtgact acacgctcgt gattccgaca
     9361 ggcggcgcgt ccacaccgat cgagatcgac tacgggccgt cgggctactg gaccacacag
     9421 gtcattcgaa acgtggcgct cgacaccaat gtgacgggtc ccaatacgat gttctggcga
     9481 atcggcgacg gcccgatctg gggcgacggg cagatgggcg cggtgtatgc ggcggccagc
     9541 ctcacctatg acctcgcgaa ggcgacgctg cagattgccg cgcgcgattg cgccggtgcg
     9601 ccgatcggcg gcgtcacggt taccgtcgag ccaccgcccg agaagctcct ctacatcaat
     9661 agcaacggcg acgtcgacgg ctcgctcacc acgtccgatg cgatgtacgg gcacgccgtc
     9721 gggttcaacg cagtcgcggg ccccacgcac atcaccgcat cgaaggacgg agtctcgatc
     9781 tacgagatgg acgtcgacgt ccaggcgggc cgctacaacg tgtaccccgt cttctacatc
     9841 ccatgaggat ctgctgcgct gctgtcctgc tctccggatg tggccgactc ggcttcgacg
     9901 cccgtgtcgc gagcgatgcg ccggacgcga cggacgccgc gcagccggtg tgtcccgtgc
     9961 aatcggttcc gtcgacgatc acggtcagcg gcgacacgtt tcgctacacg acattcacta
    10021 acatgaccgc gccgatcgcc ggtgccacgg tgcaagcgcg cgaccgcacc gatttgagca
    10081 tcctcgccac agtaacgagc gatggaagcg gcgcgtactc gctctcgtat tcgacggatg
    10141 gcgacgtaac accgcttgcg ctcatctatg ccgcgccgac gtacaacgag acgatcgtca
    10201 ttccgagcgt cgtcgatcgc gacatcatgg gcatgacggt cgccgtgttc tccgtcggag
    10261 atggtccgct gtggaacgac ggcgcgctcg acagcgtcta cagcgcgtac ggcagcggac
    10321 taccgcgcga cgcaacactc ggcacgatca acgtcgcggt tcgcgactgc gacggcaatc
    10381 cgctcgaagg cgcatccgtg acattcgaca ggacgcccga cgacttcgct taccaacgcc
    10441 cagacggcgc gttcgacttg tcgctcaccg cgaccgtcca gccgtatacg catctcgtcg
    10501 cgttcaacat gccgctcgac agctaccgcg tcagcgcgca acggcccggc tatcgcttca
    10561 ccgacgagtc gatcttcaca gatgccagtg cgacgacgct cgtcgtgatt cgcggcgcgc
    10621 tagcgccctg actgcgcgtc ttgcacgacc ttcgcgagct cgtcgatctg cgtatcgagg
    10681 gccttccggt cgggaccgtc cggcaacgca tcacgctgct tcttcagcgc cgcgatcttc
    10741 ttgttcgcca tctcgatcag ctcgcgcgtc tgctgcttcg cttctgcgag ctgctcctcg
    10801 aggctcgctt tgtcctcgag cgcggctttc tcctcggcga gcgcttcgag cagcttgcgc
    10861 tgcgcctcgg ctgcgtctgc ttcggccttt acggcggtcg ccttggcctc gtcggcttgg
    10921 cggcgcgccg tgctcagctt gtcttccttc tcgctgcagg cggttagtac gagcaacgcg
    10981 gcgagcagga agcgcacacg cagagcttac tcgatcgcca ccttcacggg cgcgacccac
    11041 gtcttgtcct cggccgtgta gtacaggtac gtcgtcgacg caggagcttc gtagctgccc
    11101 ggcgtctgcg cgagcaggtc gagctcgacg tccttctttg cattcggcgc gagcgcgcgg
    11161 aagtacacga tgacctcgcg cgggcgcgtc tcgtagaagt cgatgacgcc cttgtcgcgg
    11221 agctccttca gctgccacgt ctggaacgtg aggccgccgg ggataccgat gcgggcgagc
    11281 gtcatcggca ggccttcggc cgtcttgttc tcgatgcgcg cgcgcagctt cacgccttcg
    11341 ccgagcttca ccttctcttt cgcgagctgc gtcgtgacgt tcaccttcgc gtcggccgac
    11401 gacgccgggt tcttcgcgcg atacgtgacc gcgatcgagt acggcagcgt cgcgccaccc
    11461 gcgagcgaca gctcgatcgt gttgtgaccg ggcttgagct tgccttcgag atcatcccag
    11521 acgagcgcgt ccttgcggcc cttctcgaag tggatggagc ccgcgggctc gccgttgatc
    11581 atcagcgtcg cgtcacccga cgatgccatc tgctttgcgt agtcgctgta cgcggtcatc
    11641 gccttgaggc cgaggatcgt tgcttgcgta ttcgaccact cgccgaagcc gccgcgcttc
    11701 ttgttgagcc aatccaccga cgcgcgaatc tgcgtctcgt actcgttgtt cggcgatgcc
    11761 ttgatcatcg cgagcgtcgc gagcgcggtc gtctcgatcg tgagcgactc accgccggac
    11821 atcgtgatcg attctttggc gccctggaag ctgccgtctt tatcctgcag gccgacgagc
    11881 ttcttcagat agctgtcagc ctcgggcatc ttcgcgagcg tcgccgtgtt cgcgacgagc
    11941 gcgagcaggt atgggtcacg cgtgccctcg ccgagcttct tctgcgcggt gagctccgcg
    12001 tcgagaccct tcgtgcgatg cgcttcggag agcgcccaca tgatgtacgc gttcgtcgtc
    12061 tgctcgttcg cgcgcccgaa gttgtcgagc gcttgtgacg agcggaggaa tccacctttg
    12121 ccgtcgcgcc ggctcatgag ccaatccgcg gtccgctcga ccatcgagtg atcgacctcg
    12181 tacaccttgc ccatgtcctc gaactccatg aggccgtacg cggtgagtgc ctcgtggcct
    12241 ggcgtctgtc cgaaccactc gaagcccttc tgcttcgtct cgtagccggt gagcagcttg
    12301 tagccgcgat cgaggtgacc ttgcgtcttc tgcacgagct gcgcatcagc tgcgtcgttc
    12361 gtcgcgaggt agctgaggat catgatgttc ggatagttcg tcgccgacgt ttgctcgaag
    12421 cagccgcccg gctcgcgaat catcgcctcc atgccctgcg tcatcgacgc gaccggcgac
    12481 ggatacatcg tgaccgtcgc ttcgatcgag cctgggagcg catcggcgag ctcgaagtcg
    12541 tgcttcaccg gcgtgcccgt cttcgcggtg cccgacgcgg cgagctcgaa cgggaagccg
    12601 agtggcacga cgcggatctt cttgtcgagc ttgtcttgca ggccgcgcgc cgtgacgccg
    12661 agcgagacat cggcttcgcc ggtcgtcgcg acgacatcga gcgggaagaa cagactcttc
    12721 ttctcgtgtg ccttgaggtg gatcttgccg ctcgggttgg cgccgagctt gaatgcagag
    12781 ccgaacgccg cggtgaggtc ggcgtccatc tcctcgtcgg tctcgtttgt cagcgtgacg
    12841 ggcagcttga tcttgtcgcc gctcgtgacc tcgacgggca gatgcgcgtc gagtgacagc
    12901 ggcatcttcg attgaatgac gacatcgccc gcaccgggca gaccgcccgc cgacacaccc
    12961 tcggcggtcg cgcggaacga cgtcaccgca tcggagaccg gaaacgacac gctcgcggtg
    13021 ccgtcggcat tcgtcgagac atttgcgttc cagtagatcg tctcgc
//
LOCUS       DATLBZ010000218         8519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4684012, whole genome shotgun sequence.
ACCESSION   DATLBZ010000218 DATLBZ010000000
VERSION     DATLBZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4684012"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VMZ53_11630"
     CDS             <1..241
                     /locus_tag="VMZ53_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29159.1"
                     /translation="YNLNLDNAPFKDVVMEITFGPMGPARLKLTIDGAVIVDKAATHP
                     IPPSTLLLELGAGVSRNGMSPWAVRYDSLTVDLDR"
     gene            238..789
                     /locus_tag="VMZ53_11635"
     CDS             238..789
                     /locus_tag="VMZ53_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29160.1"
                     /translation="MRGTVLVVLAACGHAPATRPPSSPCEGAIAGFASAEPAHLVVMP
                     ATCTRAELAAMMHDDNLWTRGSLGDANATYEMGYLSSPRLKNANAFLDEHGHVRLITA
                     EMPPGTEAQYRAALGEPDVAIDYVFGDPDVVLRKGELLWLSRGVTLVVSKGLIRVGIF
                     PPTTLEQYAAKLKFIDIEDVDEG"
     gene            855..2516
                     /locus_tag="VMZ53_11640"
     CDS             855..2516
                     /locus_tag="VMZ53_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HUS29161.1"
                     /translation="MITEKKAAAKDGGAMQADAHGQADVLAGGGDHDAQTQTRYSGHG
                     QIPDVAPLRDATSPTASQRAYGEVGRSGRGERDEDASATTTTTTAAATTTGANDSGGP
                     LSDRFKGDGTLASVELDERTLKLGDTGRSVRKVQQALFDLNYGPITVNGTYDAATVAC
                     VKRFQGDHKLAKTDGELDSTTFETIERQFSSLAAYADAAHHAPPGLQHTPFKTDAKDV
                     PVHLAETRELDDEDRAEADIVISDTRTGASGKAPAAFKPEVDGKTYGQRVEELLVEKI
                     AKDKSRGQAIKAAHDKGKLFTMEAMESVGNAAKGQVDKVFGSFAEGPSFEAGTNLVDR
                     FEADTEEHAQQGPKANHRDAVRRLQYLLNQHDGFAAINHMHAANPTRDREKAILERCI
                     QNIATQHEEDVLFIISTAAANTDRETRTVSLQRHKTGKADADRKTLWKKFATMVHEYV
                     HVLAHPNWHANRTAKGKSDQQAGNTLGEGVPELLARTVLSQVNFHDKALRKAVLGSTK
                     DDGEVPEIDRGYDAPFAHAMSLVGVVGIHNVYAAYFLGETKLIGS"
     gene            2657..3085
                     /locus_tag="VMZ53_11645"
     CDS             2657..3085
                     /locus_tag="VMZ53_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29162.1"
                     /translation="MSCTLDDARQVLQPTKAQTPTYLGTSTEKVRLYFFKSEKLKIVR
                     VWVNETGHLILVDADYPPAPPEQYFQTLGEPDAHLDVHWGSGEIPGGEHVWLQRGATI
                     VAKPEWTKGVAHVGIFPAGLTLDDYQKSHRYMKITDEDEG"
     gene            complement(3082..3540)
                     /gene="ybeY"
                     /locus_tag="VMZ53_11650"
     CDS             complement(3082..3540)
                     /gene="ybeY"
                     /locus_tag="VMZ53_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HUS29163.1"
                     /translation="MPLDLAIDDGVKVPAAMRTRLRREVARMVKAAALYEGRTDLEVA
                     LRLCDDAAIHELNRDYRNKNKPTDVLAFAQREAQSADQSLLGDIVISVEAAQRQAKKG
                     LYGELLHLASHGLCHLLGYDHRNDKEERQMNARAAALRKEAARTGKPRAA"
     gene            complement(3540..4733)
                     /locus_tag="VMZ53_11655"
     CDS             complement(3540..4733)
                     /locus_tag="VMZ53_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUS29164.1"
                     /translation="MLAVPMISARAEVIGVIQLINKRAKGWIQLDLPSDFEDGVVPFD
                     DVSEAYVAALASQAGIALENVLLYEEVKTLFDSFVKAAVTAIESRDPTTSGHSERVAE
                     LTVGLARAVNRAESGHYREVRFSRDDLTQIEYAALLHDFGKVGVREHVLVKAKKLYEH
                     EREMILQRFQLIKRGFKIEGLEKKVRYLSEATREQVAAELASVDKDIVAKIGELDNII
                     TFILQANEPTVLDQGGFERIAELSGMKYRDEAGHEHPFLSPAEASCLQIRRGSLTENE
                     RLEINSHVVHTFNFLVRIPWSRTLRDVPDIAGAHHEKLDGTGYPNKLKGDQIPIPARM
                     MAISDIYDALTASDRPYKKAMPAEKALDIVGSEVKRGQLDAELFEVFVGARVYALTNR
                     GDGKS"
     gene            complement(4788..5534)
                     /locus_tag="VMZ53_11660"
     CDS             complement(4788..5534)
                     /locus_tag="VMZ53_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUS29165.1"
                     /translation="MVVTSPDDLVNAVARDRRMSGYRVRRQGDGWIELEVIAQRARSP
                     VMLVLAWPEVWKDAEALTPHLVRARSGNYGLIMVGADQELEDARLDAAPSDADLTAIS
                     SPVAMVRLLLLLRTRSEAIAMRMQAAGLELELERARHENDMLISIGRALSQERDIQSL
                     LAIILRRACEVTNADAGSVYIVEGHDEDVSKRMLRFAVSQNESRNVQSAGFTMPISAS
                     SIVGTCVLSGDRINVPDLYNLDEPGVGNNP"
     gene            complement(5537..6592)
                     /locus_tag="VMZ53_11665"
     CDS             complement(5537..6592)
                     /locus_tag="VMZ53_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HUS29166.1"
                     /translation="MAKQLHREKLAFDDPEALQALCGSNNSRLKLIERTAGAEVHLRG
                     NEITIEGDEAATSTAKTAINQLYDMALDGKPLSSDDVVRAVRLLQGTDDGAPRQTISQ
                     VFGETILTPRAGRPIAPKGATQKKYVDMVRANDIVFAIGPAGTGKTYLAMAMAVRALL
                     DKQVRRIILTRPAVEAGERLGFLPGTLEEKVDPYLRPLFDALNDMLDPERLQRMMADN
                     IVEVAPLAFLRGRTLNEAFIVLDEAQNTTPAQMKMFLTRMGYGAKVIITGDITQTDLP
                     PGQQSGLRDALELVRDIRGIGGIEFSDADVVRHPLVAALIRAYDARDRARFEERETRD
                     RERAGGAPNRDRDELDS"
     gene            6559..7422
                     /gene="mazG"
                     /locus_tag="VMZ53_11670"
     CDS             6559..7422
                     /gene="mazG"
                     /locus_tag="VMZ53_11670"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HUS29167.1"
                     /translation="MRVSRGEAAWPRTERRYSTCMQAGSSLSRLVEIMDRLLAPGGCP
                     WDREQTLDTLRPFLVEETYEVLDALVRDNAEDHCEELGDLMMQIVFQAALRREQGKFT
                     IDDVCDSISEKLIRRHPHVFAKVEGVDTPAKVLAQWDEIKDKEKAEKAAPEKKRRVLD
                     GIPPGPALARAQKIGSRCGKVGFDWPGWEGSFAKVEEEVTEVLGAVKAQDAREVHNEL
                     GDLLLAVVNLSRKLGVDAENALLDATQRFKTRFEYVEDKLVDRGKTPQQSNLEEMDAL
                     WNEAKKHTPRK"
     gene            7597..7866
                     /gene="rpsT"
                     /locus_tag="VMZ53_11675"
     CDS             7597..7866
                     /gene="rpsT"
                     /locus_tag="VMZ53_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HUS29168.1"
                     /translation="MANIASAEKQNRKMIKNRARNRAAMGSLRTAVKTARTAVDSKAA
                     NAPELVKKAVSIIDSAVTKGILKRQTASRYVSRLARRTSSTAAAR"
     gene            7912..>8519
                     /locus_tag="VMZ53_11680"
     CDS             7912..>8519
                     /locus_tag="VMZ53_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29169.1"
                     /translation="MKTYVLALVVAMGCGGSSYDPSIDPAHFVSGVDNPLFPLVPGTV
                     FQYRVQETGEEVTVTVTADTKLVMGVPCVVVRDTARSGGQIVEDTYDWYAQDDQGTVW
                     YMGEDTTAYDGGMTSKEGSWQGGVDGALPGKIIPGSPHVGDTYRQEYYEDEAEDMGQI
                     LALDASITVPAGSYVGCLQTRDFSALEPEIEENKWYCPGVGQV"
BASE COUNT         1556 a         2669 c         2846 g         1448 t
ORIGIN      
        1 ctacaacttg aatctcgaca acgctccgtt caaggacgtg gtcatggaga tcacgttcgg
       61 accgatgggc cccgcgcggt tgaagctcac gatcgatggg gccgtgatcg tcgataaggc
      121 cgcgacgcat ccgattccgc cgtcgacgtt gttgctcgag ctcggcgccg gcgtgtcgcg
      181 aaatggcatg tcgccgtggg cggtgcgcta cgacagcttg acggtcgacc tcgatcgatg
      241 aggggaacgg tgctcgtcgt tctcgcggca tgcggccacg caccagcgac gcgaccgccg
      301 tcatcgccgt gcgagggcgc gatcgctggg tttgcatcgg cggagcccgc acatctcgtc
      361 gtcatgcccg cgacctgcac gcgcgcggag ctcgcagcga tgatgcacga cgacaatctc
      421 tggactcgcg gcagcctcgg agatgccaac gcgacttacg agatggggta tctgtcgtca
      481 cctcgactga agaacgcgaa cgcgtttctc gacgagcacg ggcacgtgag actcatcacc
      541 gccgagatgc cgccggggac ggaggcgcag taccgcgccg cgttgggcga gccggacgtg
      601 gccatcgatt atgtgttcgg cgacccggac gtcgtgcttc gcaagggcga gctgctgtgg
      661 ctgtcgcgcg gcgtaacgct cgtggtctcg aagggcttga tccgcgttgg aatctttccg
      721 ccgacgacgc tcgagcagta cgccgcgaag ctcaagttca tcgacatcga ggacgtcgac
      781 gagggatagg aatctgtggc cgatcgctgc gatttcgggc atctcgggca gtcggcgcac
      841 gtatattggc ggtggtgatc acggagaaga aggcggcggc caaggacggc ggggcgatgc
      901 aggcggacgc gcatgggcaa gcggacgtgc ttgccggcgg cggcgaccac gacgcgcaga
      961 cgcagacgcg gtactcgggt cacggccaga ttcctgacgt cgcgccactg cgtgatgcaa
     1021 cgtcgcccac cgcgagccag cgcgcgtacg gcgaggttgg ccgcagcggc cgcggtgagc
     1081 gcgacgaaga cgcgtcggcg acgacgacga cgacgaccgc ggcggcgacc acgaccggag
     1141 cgaatgacag cggcgggccg ctcagcgatc gcttcaaggg cgacggtacg cttgcgtctg
     1201 tcgagctcga cgagcggacg ctgaagctcg gcgataccgg gcgctcggtc cgcaaggtcc
     1261 agcaggcgct gttcgatctg aattatggcc ccatcaccgt caacgggacg tacgacgcgg
     1321 cgaccgtcgc gtgcgtcaag aggttccagg gcgaccacaa gctcgcgaaa acggatggcg
     1381 agctcgactc gacgacgttc gagacgatcg agcggcagtt ctcgagcctc gctgcgtacg
     1441 cggacgctgc gcatcatgcg ccgcctggcc tgcagcacac gccgttcaag acagacgcga
     1501 aagacgtgcc cgtgcacctc gccgagacgc gcgagctcga cgacgaagat cgcgcagaag
     1561 ccgacatcgt catcagtgac acgaggaccg gagcgagcgg caaggcgccg gcggccttca
     1621 aaccggaggt cgacggcaag acgtatggcc agcgcgtaga ggagctgctc gtcgagaaga
     1681 tcgcgaagga caagtcgaga ggccaggcga tcaaggcggc gcacgacaag ggcaagctct
     1741 tcacgatgga ggccatggag agcgtcggaa acgccgcgaa gggccaggtc gacaaggtct
     1801 tcggcagctt cgccgaaggg ccgagcttcg aagctgggac gaacctcgtc gatcgtttcg
     1861 aggccgacac cgaggagcac gcgcagcaag gacctaaggc gaaccatcgc gacgccgtcc
     1921 gccggttgca gtatctgctc aaccagcacg acggattcgc cgcgatcaat cacatgcacg
     1981 ccgccaatcc gacgcgagat cgcgagaagg caatcctcga gcgatgcatt cagaacatcg
     2041 ctacgcagca cgaggaagac gtgctcttca tcatctcgac agcagccgcg aacaccgatc
     2101 gagagacgcg taccgtcagc cttcagcgac acaagacggg taaggcggac gccgaccgga
     2161 agacgttgtg gaagaagttc gccacgatgg tccacgagta cgtccacgtc ctcgcgcatc
     2221 cgaactggca cgcgaatcga acagcgaagg gcaagtccga ccagcaggcc ggcaacacgc
     2281 tcggcgaagg tgtgccggag ctgctcgctc gcaccgtcct atcgcaggtc aattttcacg
     2341 acaaggcgct tcgcaaggcg gtccttggct cgacgaagga cgacggcgag gtgcccgaga
     2401 tcgatcgcgg ctacgacgca cccttcgcgc acgcgatgtc actcgtcggc gttgtaggca
     2461 ttcacaacgt ctatgcggcg tactttctcg gcgagacgaa gttgatcggt tcatgatgcg
     2521 tggcgctgct gtcgcgatca ttgtcgctac cgcctgctgt tcgcatccga agggcccggc
     2581 catgtcgaaa tcagaatgtg ctctcgcgat agaaggcttc gcgtcgaccg aaccagcgcg
     2641 acttcgcgcg ctgcctttga gctgcaccct cgacgacgcg cggcaggtcc tgcaaccaac
     2701 gaaggcgcag acgcctacgt acctcggcac gagcaccgaa aaggtccgcc tctacttctt
     2761 caagtccgag aagctgaaga tcgtccgcgt ttgggtgaac gagaccggtc acctcatcct
     2821 cgtcgatgcg gactatcccc cggcaccgcc agagcagtac ttccagacgc tcggtgagcc
     2881 agacgctcat ctcgacgttc actggggatc cggagagatt cccggcggcg agcacgtctg
     2941 gctgcagcgc ggcgcgacga tcgtagcgaa gccggagtgg acgaaaggtg tcgcgcacgt
     3001 cggcatcttc ccggccgggc tgacgctcga cgactaccag aagtcgcacc gctacatgaa
     3061 gatcacggac gaagacgaag gctaggccgc acgaggcttg ccggtgcgag cagcttcttt
     3121 gcgcagcgcc gcggcacgcg cgttcatctg ccgctcttct ttgtcgttgc ggtgatcgta
     3181 gccgagcaag tggcagagcc catgcgacgc gagatggagg agctcgccgt agaggccctt
     3241 cttcgcctgg cgctgtgcgg cctcgacgga gatcacgatg tcgccgagca gcgactgatc
     3301 cgcggactgc gcctcgcgct gcgcgaatgc gagcacgtcg gtcggcttgt tcttattgcg
     3361 gtagtcgcgg ttcagctcgt ggattgcggc gtcgtcgcat aggcgcaacg cgacctcgag
     3421 atccgtgcgg ccctcgtaga gcgccgccgc cttgaccatg cgcgcgacct cgcgacgcag
     3481 gcgcgtacgc atcgcggccg gcactttcac gccgtcatcg atcgcgagat cgagaggcac
     3541 tacgacttgc cgtcgccacg attcgtcagc gcgtagacgc gggcgccgac gaacacttcg
     3601 aacagctcgg catcgagctg gccgcgcttc acttccgaac cgacgatatc gagcgccttc
     3661 tccgcgggca tcgccttctt gtacgggcga tcgctcgcag tgagcgcgtc atagatatcc
     3721 gagatggcca tcatgcgggc ggggattgga atctggtcgc ccttgagctt gttcgggtag
     3781 cccgtaccgt cgagcttctc gtggtgcgca cccgcgatgt cggggacgtc gcgcagcgtg
     3841 cgactccagg ggatgcggac gaggaagttg aacgtgtgga cgacgtgcga gttgatctcg
     3901 aggcgctcgt tctcggtcag cgagccgcgg cggatctgca ggcacgacgc ttcggcgggc
     3961 gacaggaacg ggtgctcgtg tcccgcttcg tcgcggtact tcatcccgct gagctccgcg
     4021 atgcgctcga agccgccttg gtcgaggacc gtgggctcgt ttgcttgcag gatgaacgtg
     4081 atgatgttgt cgagctcgcc gatcttcgcg acgatgtctt tgtcgacgga ggcgagctcg
     4141 gcagcgactt gctcgcgcgt ggcttcggag aggtagcgga ccttcttctc gagcccttcg
     4201 atcttgaagc cgcgcttgat gagctggaag cgctgcagga tcatctcgcg ctcgtgctcg
     4261 tagagcttct tcgctttgac gaggacgtgc tcgcgcacgc cgaccttgcc gaagtcgtgc
     4321 agcagcgcgg cgtactcgat ttgcgtgagg tcgtcgcgcg agaagcggac ttcgcggtag
     4381 tggccgctct cggcgcggtt gaccgcgcgt gcgaggccga ccgtcagctc ggcgacgcgc
     4441 tcggagtgac ccgacgtcgt cggatcgcgc gactcgatcg cggtgaccgc ggcttttacg
     4501 aagctgtcga acagcgtctt gacctcttcg tagaggagga cgttctcgag cgcgatgcct
     4561 gcttgcgatg cgagcgcggc gacgtatgcc tccgacacat cgtcgaacgg cacgacgccg
     4621 tcttcgaagt cggatggcag gtcgagctgg atccagccct tcgcgcgctt gttgatcagc
     4681 tggatgacgc cgatcacttc ggcgcgggcg ctgatcatcg gcaccgcgag caccgagcgc
     4741 gtctggtagc ggtacttgtc gtcgaacgag cggtcgtgga cgaagcccta cgggttattg
     4801 ccgacgccgg gctcgtcgag gttgtagaga tcgggcacgt tgatgcgatc gcccgagagc
     4861 acgcacgtgc cgacgatcga cgacgcgctg atgggcatcg tgaagccggc cgactgcacg
     4921 ttgcgcgact cgttttgcga gaccgcgaag cgcagcatgc gcttggagac atcctcgtcg
     4981 tggccttcga cgatgtagac cgagccggcg tccgcgttcg ttacctcgca cgcgcggcgc
     5041 aagatgatcg cgaggagcga ctggatgtcg cgttcttgcg agagcgcgcg gccgatcgag
     5101 atgagcatgt cattctcgtg acgcgcgcgt tcgagctcga gctcgagccc agcagcttgc
     5161 atgcgcatcg cgatcgcctc ggagcgcgtc cgcaacagga gcagcaggcg caccatcgcg
     5221 acaggcgacg agatcgccgt caggtcggca tcgctcggcg cagcatccag gcgcgcatct
     5281 tcgagctctt gatcggcgcc gaccatgatg aggccgtagt tcccggagcg cgcgcgaacg
     5341 agatgcggcg tcagcgcttc ggcgtccttc cagacctcgg gccatgcgag cacgagcatc
     5401 accgggctgc gcgcacgctg cgcgatcacc tcgagctcga tccagccatc gccctgacgg
     5461 cgcacgcgat atccggacat ccgccggtca cgcgcgaccg cgttgaccag gtcgtctgga
     5521 ctggtcacca ccatgcttac gagtctaact cgtcgcggtc gcgattgggc gccccgcccg
     5581 cacgttcgcg gtcgcgggtt tctctctcct cgaaccgcgc acgatcacgg gcgtcgtacg
     5641 cgcggatgag ggctgcgacg aggggatgtc gcacgacatc ggcgtcggag aactcgatcc
     5701 cgccgatgcc gcgaatatca cggacgagct cgagcgcgtc gcgcaggccc gattgctgac
     5761 cgggcggcag gtccgtctgc gtgatgtcac ccgtgatgat gaccttggcg ccgtagccca
     5821 tgcgcgtcag gaacatcttc atctgcgcgg gcgtcgtgtt ctgcgcctcg tcgaggacga
     5881 tgaacgcctc gttgagcgtg cgaccgcgaa ggaatgcgag cggcgcgacc tcgacgatgt
     5941 tgtccgccat catgcgctgg aggcgctcgg gatcgagcat gtcgttgagc gcgtcgaaca
     6001 gcgggcgcag gtatgggtcg accttctcct cgagcgtgcc gggcagaaag ccgaggcgct
     6061 cgccggcttc gacggccgga cgcgtgagga tgatgcggcg aacctgcttg tcgagtagcg
     6121 cgcggacggc catcgccatt gcgagatacg tcttcccggt gcccgcgggg ccgatcgcga
     6181 acacgatgtc gttcgcgcga accatgtcga cgtacttctt ctgcgtcgcg cccttgggtg
     6241 cgatcgggcg acctgcgcgc ggcgtcagga tcgtttcgcc gaacacttgg ctgatcgtct
     6301 ggcgcggcgc gccatcgtcg gtgccttgca gcaggcggac ggcgcgcacc acgtcgtcgc
     6361 tcgacagcgg cttgccgtcg agggccatgt cgtagagctg attgatggcg gtcttcgccg
     6421 tggacgtcgc agcctcgtcg ccctcgatcg tgatctcgtt gccgcgcagg tggacttcgg
     6481 cccctgccgt acgctcgatc agcttgagcc ggctgttgtt gctcccgcag agcgcttgga
     6541 gcgcttcggg atcatcgaat gcgagtttct cgcggtgaag ctgcttggcc acggacggag
     6601 cggcggtata gcacgtgtat gcaggctgga agctcgctct cccgtctcgt cgaaatcatg
     6661 gaccgtttgc tcgcgccggg cggctgtccg tgggaccgcg agcagacgct cgatacgctc
     6721 cggccattcc tcgtcgagga gacgtatgaa gtcctcgacg cgctcgtgcg cgacaacgcg
     6781 gaggatcact gcgaagagct cggcgacctg atgatgcaga tcgtgtttca ggcggcgctg
     6841 cgccgcgagc aggggaagtt cacgatcgac gacgtctgcg acagcatttc ggagaagctg
     6901 atccgccggc acccgcacgt gttcgcgaag gtcgaaggcg tggatacccc tgcgaaggtc
     6961 ctcgcgcagt gggacgagat caaagacaag gagaaggccg agaaggcggc cccagagaag
     7021 aagcggcgcg tcctcgacgg catcccgccc ggccctgccc tcgcccgcgc gcagaagatc
     7081 gggagtcgct gcggcaaggt cggcttcgat tggccaggct gggaaggctc gttcgccaaa
     7141 gtcgaagaag aagtcaccga ggtgctcggc gccgtgaagg cgcaagacgc tcgcgaggtc
     7201 cacaacgagc tcggcgacct gttgctcgcg gtcgtgaacc tgagtcgcaa gctcggcgtc
     7261 gatgcggaaa acgcgctgct cgatgccact cagcgattca aaactcggtt cgagtacgtc
     7321 gaggacaagc tcgtcgatcg tggcaagacg ccacaacaat ccaacctcga ggagatggac
     7381 gcgctgtgga acgaggcgaa gaaacacact ccgagaaagt gatgcttttt tgtgcagccg
     7441 agatcgactc gaagggatat cggccagatc cccgttagga tcaccaagca ttccccagct
     7501 tatccacagc ggttagtcgg cgaactaccc tcatccactg ggcattgaca cccctgcccc
     7561 cagtggggta cacgctcctc cctccaggag aacccagtgg ccaatatcgc ctcggccgag
     7621 aaacagaacc gcaagatgat caagaatcgc gcgcgcaacc gcgccgcgat gggatcgttg
     7681 cgcaccgccg tgaagaccgc ccgcaccgcc gtggacagca aagctgccaa cgcgccggag
     7741 ctcgtgaaga aggccgtgtc gatcatcgac agcgccgtca cgaagggcat cctcaagcgt
     7801 cagactgctt ctcgctacgt ctcgcgtctc gctcgtcgca ccagcagcac cgcagccgct
     7861 cgctgagccg gcgctcatct tggcttcatg ttgacggcgt aggctgtcga catgaagaca
     7921 tacgtgctcg cattggtcgt ggcgatgggt tgcggagggt cgagctacga tccttcgatc
     7981 gatccggcgc acttcgtcag tggcgtcgac aacccgctgt tcccgctcgt gccagggacg
     8041 gtctttcagt atcgcgtgca ggagaccggc gaagaggtca cggtcaccgt cacggcggat
     8101 accaagctcg tgatgggcgt gccgtgcgtc gtcgtgcgcg acaccgcgcg ctcgggcggc
     8161 cagatcgtcg aagacaccta cgactggtac gcgcaggacg atcaaggaac cgtctggtac
     8221 atgggcgagg acacgaccgc gtacgacggc ggcatgacga gcaaagaagg ctcgtggcaa
     8281 ggcggtgtcg acggcgcgct gcccggcaag atcatccctg gctcgccgca tgtcggtgac
     8341 acataccgcc aggagtatta cgaagacgaa gccgaggaca tgggtcagat cctcgcgctc
     8401 gatgcatcga tcacggtgcc cgcaggctcg tacgtcggct gcctgcagac gcgtgacttc
     8461 tccgcgctcg agccggagat cgaagagaac aagtggtact gccccggtgt cggtcaggt
//
LOCUS       DATLBZ010000219         2529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10545955, whole genome shotgun sequence.
ACCESSION   DATLBZ010000219 DATLBZ010000000
VERSION     DATLBZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10545955"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VMZ53_11685"
     CDS             <1..652
                     /locus_tag="VMZ53_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29170.1"
                     /translation="KVLKGLNHQLTNRLAALDAVSQLFEPGETTDPQLVSTMAREVER
                     VATLLRLYRMLPAEPTALPEAVRVQDIVPGVLELHTYHADLRGVACVMGEASDPAPVC
                     VRPSALLRSLLVLLESAAGHSYRTGSGLPIVLSYDGTPTTVTLTIEAPSPNADGLFAG
                     SGSLIDAVRSALAHAGGKINAGRDAREGLPLIKYCLTLPTLREVRRKGAIATKAEA"
     gene            complement(649..1389)
                     /locus_tag="VMZ53_11690"
     CDS             complement(649..1389)
                     /locus_tag="VMZ53_11690"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HUS29171.1"
                     /translation="MPSTLARRSRTTDDANRDAILRQHLGLVYHVAQQLTRARANDVE
                     LDDLVSAGTLGLIDAFENFDTTRGLAFSTFAAPRIRGAMLDELRRLDRVPRSVRRKTR
                     QIDGACTALAGVLGREPGHAELAEGLGIDLATFWRWQTERESTQVVSIDRAPSSSDDG
                     RGAGEWLAGETGDEADESITLAQEAAVLRDALLEIPEQERTVLSLYYFEELKLNDIAR
                     VLQVSESRVSQIRSKAIQRLRRRLANLR"
     gene            1822..2130
                     /locus_tag="VMZ53_11695"
     CDS             1822..2130
                     /locus_tag="VMZ53_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29172.1"
                     /translation="MEKLPMRDEHVLPIDRENAGRGDKGTGRENDPSSAQADGITADL
                     RTDAPAPLSAASHPADAEDPGLSDEEIATYRQRVADGMYNTREVADEVARRMLRRGDI
                     "
     gene            2191..2415
                     /locus_tag="VMZ53_11700"
     CDS             2191..2415
                     /locus_tag="VMZ53_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29173.1"
                     /translation="MKDRTNDQPGAQRHAEGTQGKATREANRERIASQPDDDNVINHG
                     PDGRHRIHEDRQQHDEADKNSEKNRLDREK"
BASE COUNT          463 a          836 c          820 g          410 t
ORIGIN      
        1 gaaagtgctc aaggggctga accaccagct gaccaaccgg ctggccgcgc tcgacgccgt
       61 ctcccaactg ttcgagccgg gcgagacaac cgatccgcag ctcgtctcca ccatggcgcg
      121 cgaagtcgag cgcgttgcaa cccttctgcg gctctaccgg atgcttcccg ccgagccgac
      181 cgcgttgccc gaggcggtgc gcgttcagga catcgtgccg ggcgtgctcg agctccatac
      241 ctaccacgcg gatcttcgcg gcgtcgcctg tgtgatgggc gaggcgagcg atcccgcgcc
      301 ggtgtgcgtg cgaccgtcag cgctgctgcg atccctgctc gtcctcctcg agagcgccgc
      361 gggtcattcc taccgcaccg gcagcgggct cccgatcgtg ctgtcctacg acggtacgcc
      421 gacgaccgtc acgctgacga tcgaagcgcc gtcgcctaac gcggacgggc tgttcgccgg
      481 cagcggcagt ctcatcgacg ccgtgcgcag cgcgctcgcg cacgcgggcg gaaagatcaa
      541 cgccgggcgc gatgcgcgcg aagggcttcc gctgatcaag tactgcctga cgctgccgac
      601 gttgcgtgag gtccgtcgca agggcgccat cgcgacgaaa gcggaagcct aacgaaggtt
      661 cgccagccgc cgccgcagcc gctggatcgc cttcgaccga atctgcgaca cacgcgactc
      721 gctcacctgc agcactcgcg cgatgtcgtt cagcttgagc tcttcgaagt agtagagcga
      781 cagcaccgta cgctcctgct cgggaatctc gagcagcgcg tcacgaagca ccgcggcctc
      841 ctgcgcgagc gtgatcgact catcggcctc atcgccggtt tcgcctgcga gccattcgcc
      901 ggcaccgcgc ccatcatctg agctgctcgg cgcgcgatcg atcgatacga cctgcgtgga
      961 ttcgcgctcg gtctgccacc gccaaaacgt cgcgagatcg atgccgagtc cttcagccag
     1021 ctccgcgtgg cctggctcgc gaccgagcac gcccgcgaga gcggtgcagg cgccgtcgat
     1081 ctgtcgcgtc ttccggcgta ccgagcgcgg aacgcggtcg agccgacgca gttcgtccag
     1141 catcgcgccg cgaatgcggg gcgcggcgaa ggtgctgaac gccaggccgc gcgtcgtgtc
     1201 gaagttttcg aacgcgtcga tgaggccgag ggttcccgca cttaccaggt cgtcgagctc
     1261 cacatcgttc gcccgtgccc gcgtgagctg ctgggcgacg tgatacacga ggccaaggtg
     1321 ctggcgcagg atggcatcgc gattggcgtc gtccgtggtg cgcgagcggc gagcgagagt
     1381 gcttggcatc gactacgtct gctccaacgg gggacctgga attcacgagc gtgcgcatag
     1441 ccgtccgcga cgctccgcat gttcacggag agcgtctcaa ggcaaccgac gttccagggc
     1501 tgccggcact gcgcaagcga gcgttcggat gttcgtagcc gtcgcataag acgttggcag
     1561 gcaacgcgct atggacaggc gagggcggtt ggtacgcgtc gcgatctgct cgcacgcgag
     1621 gcactctctt gacgaatcac gctgcacatc gttccgtccg gcaacgattg ccgactaaac
     1681 cgcgtcggcc tcgtgccgat actgagagac acagatcaca acgaccggat cgggagttgc
     1741 gatctcagcg cggagcggcg tcgaacacgg ggcgcttcag tttccggctt cgaggccgat
     1801 acctgatctt ggaaatcact tatggagaag ctgccgatgc gcgacgagca tgtgctgccg
     1861 atcgaccgcg agaacgcagg gcgcggcgac aagggcaccg gacgcgagaa cgacccatcg
     1921 tcagcacagg cggatggcat caccgccgat ctccgcactg atgcgccggc tccgctgtcg
     1981 gccgcttcac atcctgccga cgccgaggat ccgggtttga gcgacgaaga gattgcgacg
     2041 tatcgccagc gtgtggcgga cggcatgtac aacacgcgcg aagtcgccga cgaggttgct
     2101 cgccgcatgc tccgccgcgg cgacatctga tcgacagcac ggcagtcggc atatcgattg
     2161 cccgacgctt tctcccaacg aggaggcgtc atgaaggatc ggacaaacga ccagccgggc
     2221 gcgcagcgcc atgcggaagg gacccagggc aaagcaaccc gtgaagcgaa ccgcgagcga
     2281 attgcgtcgc agcctgacga cgacaacgtg atcaaccacg gccccgacgg ccgtcatcgc
     2341 attcacgagg accgtcagca gcacgacgag gcggacaaga acagcgagaa gaatcggctc
     2401 gaccgcgaga aatagcgtcg gttttcaagg ggccaggtac gttgattcaa tcaaggtacc
     2461 tggccccttg aaaactgaag ctatttctcg cgatcgaggc ggttcttctc gctgttcttg
     2521 tcggcctcg
//
LOCUS       DATLBZ010000220         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3944391, whole genome shotgun sequence.
ACCESSION   DATLBZ010000220 DATLBZ010000000
VERSION     DATLBZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3944391"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..601
                     /gene="queF"
                     /locus_tag="VMZ53_11705"
     CDS             248..601
                     /gene="queF"
                     /locus_tag="VMZ53_11705"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HUS29174.1"
                     /translation="MTTTPSKQLDTFPNPRRVRDYHIVFDCPEFTCLCPMTGQPDFAT
                     ILIDYVPDELCVELKSLKLYLWSYRNEGAFHEAVTNQICDDLVAAISPRSITVTGKFW
                     VRGGITTSVVVTHSK"
     gene            complement(630..1931)
                     /locus_tag="VMZ53_11710"
     CDS             complement(630..1931)
                     /locus_tag="VMZ53_11710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HUS29175.1"
                     /translation="MSGEIAHPLIVPLPRLVAPAGRALRWAALRQLRSTWAGGRLDLT
                     LPSGECVRLGDEHGPADARARILDDRFFLRMMMRGEIGAGESFVAGEWTSDDLVGTLR
                     VFLRGTRARGIESPLTKIAQLPALLRHRRSANTHVGSERNVHAHYDLGNPFYRTFLDD
                     ETLAYSAAYFVRAHHGDASAHRDDARGGIDLTMSLADAQRAKLERLCEWLELSPRDHL
                     LEIGCGWGGMAIHAARTRGCRVTAITVSREQFELASARVAAAGLADRVSSEYRDYRTL
                     ATSGGSAFTKIVSIEMLEAVGYEYLAEYFAICARVLPRGGRFAVQTISMPDERFDAYR
                     RRVDWMQTYIFPGTLIPSVAAIRAASAPAGLELVRVDDIGLHYAPTLRMWRERFVAAL
                     PKVRALGFDTPFIRTWLLYLAFSEAAFAERTLGDHQLLFTR"
     gene            2137..>2318
                     /locus_tag="VMZ53_11715"
     CDS             2137..>2318
                     /locus_tag="VMZ53_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29176.1"
                     /translation="MPGTKSLRSVPAGPSESRVDGRLASMVVRPSRVDGRLASMVVRP
                     SRVDDRRASKVGSRRWS"
BASE COUNT          412 a          814 c          726 g          366 t
ORIGIN      
        1 gcgttcttcc tcccctggcc ttagccagaa gcccgacggc acggcccgcc agcagaattg
       61 cagcggctcc cgtcatcggg ccttcatgat gggcacttcg gcccgtctcg aagcccactt
      121 ctcgtgatgt ccacatggcc accgcgtgtg ttctcgacgg ccagaaccgg ccgcactttc
      181 agtctagggc ataccgatat ccagcgccag cctccggagt tggcattcgt gctaatcacc
      241 ccgctgcgtg acgacgaccc cgagcaaaca gctggacacg ttcccgaatc cgcgaagggt
      301 ccgcgactac cacatcgtct tcgactgccc cgagttcacc tgcctctgcc cgatgaccgg
      361 gcagccggat ttcgccacga ttctcatcga ctacgttccg gatgagctct gcgtcgagct
      421 taaaagcctc aagctctacc tttggagcta ccgcaacgag ggcgccttcc acgaagcggt
      481 caccaaccag atctgcgacg acctcgtagc cgcgatctcg ccgcgatcga tcacggtgac
      541 cggcaaattc tgggtgcgcg gcggcatcac gacgtcggtc gtcgtgacgc acagcaagta
      601 gctgtcgccc gcagaccaac gcgccggcgc taccgcgtga acagcagctg gtgatcgccg
      661 agcgtccgct ccgcgaaggc cgcttcgctg aacgccaagt acagaagcca cgtccgaatg
      721 aacggcgtgt cgaagccgag cgcgcggacc ttcggcaacg cggcgacaaa tcgctcgcgc
      781 cacatgcgca acgtcggcgc gtagtgcaag ccgatatcgt cgacacgcac gagctcgagt
      841 cccgccggtg ccgaggccgc gcggatcgcc gcgaccgacg gaatcagcgt gcccggaaaa
      901 atgtacgtct gcatccagtc gacgcgcctg cgatacgcgt cgaagcgttc gtcgggcatc
      961 gagatggttt gcaccgcgaa ccgcccgccg cgcggcagca cgcgcgcaca gatcgcgaag
     1021 tactccgcga ggtactcgta ccccacggcc tcgagcatct cgatcgagac gatcttcgtg
     1081 aacgcgctgc ctccgctcgt cgccagcgtc cgatagtcgc gatattcgct cgagacgcga
     1141 tcggcgaggc ccgccgcggc gacgcgcgcg gacgcaagct cgaactgctc gcgcgatacc
     1201 gtgatcgccg tgacgcgaca accgcgcgtg cgcgccgcat ggatcgccat gccgccccac
     1261 ccgcagccga tctcgagcag atgatcgcgg ggcgacagct ccagccactc gcagagccgc
     1321 tcgagcttcg cgcgctgcgc gtcggcaagc gacatcgtca aatcgatgcc gccacgcgca
     1381 tcgtcgcgat gggcagacgc atcgccgtga tgcgcccgca cgaagtacgc ggccgagtag
     1441 gcgagtgtct cgtcgtcgag aaacgtccgg tagaacggat tcccgagatc gtagtgcgcg
     1501 tggacgttgc gctcgctacc cacgtgggta ttcgcggagc gccggtgccg caagagcgca
     1561 ggcagctgcg cgatcttcgt cagcggtgat tcgatgccgc gcgcacgcgt gccgcgcaaa
     1621 aagacgcgca gcgttccgac gaggtcatca ctcgtccact cacccgcgac gaacgactcg
     1681 ccggcgccga tctcaccgcg catcatcatg cgcagaaaaa aacgatcgtc gaggatgcga
     1741 gcgcgtgcgt cggctggccc gtgctcgtcg ccgagtcgaa cgcattcgcc cgacggcaac
     1801 gtgaggtcaa ggcggccacc agcccacgtc gatcgtagct gccgcagcgc tgcccatcgc
     1861 agcgcgcgac ctgccggcgc gacgaggcgc ggcagcggaa cgatcaaggg atgagcgatt
     1921 tccccggaca cccgacagca cactactacg accgacgcgc cgcgcacatc gcgccgcgtg
     1981 cttgcgccgc gtgcattgcc gtccgcgtcg cgcgcggtgc gaaggtcgcg aggcgccgcc
     2041 tcctaccatc ggggtgtctt ttcggaaccc gtcatgacgc cgtcatgaca ctcaagtgtg
     2101 cgaacacccg tcgatgagcg cacgacgcgc gagtctgtgc ctgggactaa atcgctccgt
     2161 tcggtgcccg cgggaccgtc ggagtcgcgt gtcgacggtc ggctcgcgtc gatggtcgtg
     2221 cgaccgtcgc gtgtcgacgg tcggctcgcg tcgatggtcg tgcgaccgtc gcgtgtcgac
     2281 gatcgtcgcg cgtcgaaggt cggctcgcgt cgatggtc
//
LOCUS       DATLBZ010000221         5566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1712234, whole genome shotgun sequence.
ACCESSION   DATLBZ010000221 DATLBZ010000000
VERSION     DATLBZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712234"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..627)
                     /locus_tag="VMZ53_11720"
     CDS             complement(190..627)
                     /locus_tag="VMZ53_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29177.1"
                     /translation="MQIRDLIPAPIVPLFDFVCWVGSVTLATAIFWMLFGTFVLGGCI
                     DTPLPDVEPQARLLASWDPLSCGDPHRVVIELEDDAGAKLSSSVPCEIGEMHFDIWHW
                     GIYRGRIYAWMLDAGSGAVIRSEKPVRLEIDAPIVQWLVETPR"
     gene            827..1696
                     /locus_tag="VMZ53_11725"
     CDS             827..1696
                     /locus_tag="VMZ53_11725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS29178.1"
                     /translation="MSTSTLEGNREAIEAWNTVLFDKFVRYREILSKALGAHGDRGME
                     KLGVHRGARVVDIGCGFGDTTVQLGELVGPEGRAVGVDAAKSFIESARREYAAHRNVS
                     FEVADVEASVPGGPYDVAYSRMGTMFFASPVFALRNTRKTLVPGGRLSMVVWRKKETN
                     DCFYPAELAVRELLGDPPKNDQVTCGPGPFSMASPDLVSDQLIGAGFANISFERSDAL
                     MKIGRTIEEAVEFALMVGPAGEVVRLAGDAAVSRRAEIEAAVRKVVEPYATADGVMAP
                     SSTWIVTATNPAT"
     gene            complement(1697..3241)
                     /locus_tag="VMZ53_11730"
     CDS             complement(1697..3241)
                     /locus_tag="VMZ53_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS29179.1"
                     /translation="MSKADVPSEQDIINALADDLPVAVWVARAPMGEEIYTNHEFGEI
                     MGQHLAHVRVGGYTEPYGILTRDGRPYPEEKLPFVRALVEKRVVVVDDIMIRRPDRTI
                     RHVRASARPVEKAGVVSHVIVAFIDITREVEAEHAREESETRLRRAQRLEAIGTLAGG
                     IAHDFNNLIFGIKLLAAELAASESDPQRLASLKMIDDVTERSAMLTRSLLGFARKGKH
                     RAMPVAINDVVESMRELLTRTLTGIQISFELGAQDRGTVIGDASQLEQVVMNLVVNAR
                     DAVKDNGGQVIVRTSTAGERVLLDVLDDGPGIPHEIRERVFEPYFTTKTHGSQRGTGL
                     GLATVFGIAESHGGTVEVGDGIGTGAMLRVSLPASKKPAEAPAIPEAAATVEPGTGTI
                     LVVDDDRLVRRAVANTLRSLGYNTFEAMNGDEALRLFREHRDIISAVVLDMMMPGMSG
                     SSTFMAMRDIDPTVAVLLMSGYSVNEDVQGLLDAGASGFVTKPYSPDVLARSLASAIQ
                     ARSAQS"
     gene            complement(3325..3906)
                     /locus_tag="VMZ53_11735"
     CDS             complement(3325..3906)
                     /locus_tag="VMZ53_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUS29180.1"
                     /translation="MNTFKADLEEIRRRARDKMEEGAVTASYGADREKVIEVLNEVLA
                     TEIVCTLRYKNHYYMAHGIHAQSVAAEFLEHAQEEEVHVEQVAKRITELNGRPNFNPD
                     GLKTRSHAEYAEGETLDEMIREDLVAERIAIATYSEIIRWLANDDPTTRRMIEEILAK
                     EEEHADDLANLLGHLGAGNASANGNGNGNGAAH"
     gene            4028..5359
                     /locus_tag="VMZ53_11740"
     CDS             4028..5359
                     /locus_tag="VMZ53_11740"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HUS29181.1"
                     /translation="MLRWPGNILHFFFLLLVLLLAGFMYGIARLGIFLFVWGPRRTTA
                     VGTLRGWILKQSMSSLGATFIKLGQVMSTRPDLFAPEVIEQLRSLQDRIPPFAFRKVK
                     AVIESDFGKPLHDVFSEFDEKPVAAASVAQVHRARIKGGDEVAVKILRPGVRRQVERD
                     QAILILFARVLALRPTWRRSDPVGHTRHFIEAIHDQTDLGIERDNYRRFHANFANSPT
                     VRFPKVYEDLCTPRVLTMEFIRGVKVDALPPGKYPKLAETVRMTFFKMCFDHGFVHAD
                     LHPGNMVVQDETKGELVIYDVGLAKLLHEDVLIQFIDMTKCIAMGTPDDIVAHLKRFH
                     TYIGEIDWAALRVEVTAFAENFRGKDTAKLEYGELIGDMFAIGRKHKVRPVTDMMLVF
                     VALITAQGIGKMLEPEHNVFGAVAQFLIPILMKRNESVPETPEAKAARATA"
BASE COUNT          974 a         1927 c         1695 g          970 t
ORIGIN      
        1 gcgtgtagtc ggtgggcttt gacgtgtcga tgcccaacgc ctccgcggcg agattcgaca
       61 ggatcgtcat cccggatcct gtgtcgatga agccgccaac tactcggcgt tttttcgctt
      121 gcaccgtcac gtttaggtac atgcgtccca cgggcatacc tccagcctag cagccaccgt
      181 ccgcacgacc tagcgcggcg tttcgacgag ccactgcacg atcggcgcat cgatctcgag
      241 ccgcaccggc ttctccgatc ggatcacggc ccccgagccg gcgtcgagca tccacgcgta
      301 gatgcgccct cgatagatgc cccaatgcca gatgtcgaaa tgcatctcgc cgatctcgca
      361 cggtaccgac gacgagagct tcgcgccggc atcgtcctcg agctcgatca ctacgcgatg
      421 cggatcgccg cacgagagcg ggtcccacga cgcgagcagc cgcgcttgcg gttcgacatc
      481 gggcagcggt gtgtcgatgc aaccgccgag cacgaacgta ccgaacagca tccagaagat
      541 cgcggtggca agcgtcacgg agccgaccca gcacacgaag tcgaagagag gaacgatagg
      601 agcgggaatg agatcgcgaa tctgcatggc ggcgcgtctt cgcaaccggc gggccagcga
      661 cgtgacgcgc gaagctcgcg agatcatgag ggcggcgacg gcactttgtc cggtgtccga
      721 cggtgggcgg acagcgcgtc cggctgccgg ttacggcgca tgcattggac tgttccgtcg
      781 aagtcacgat tcgttacgtc ggttgttacg cctatagtgc ggacctatga gcacgagcac
      841 gttggagggg aatcgagagg cgatcgaggc gtggaacacg gtgctgttcg acaagttcgt
      901 gcgctaccgc gaaatcctga gcaaagcgct cggcgcgcat ggcgatcgcg gcatggagaa
      961 gctcggcgtg caccgcggcg ctcgcgtcgt cgacatcggg tgcggcttcg gcgatacgac
     1021 ggtgcagctc ggcgagctcg tcggacctga aggtcgtgca gtcggcgtcg atgcggcgaa
     1081 gagcttcatc gagagtgcgc gccgggaata cgcggcacac cgaaacgtca gcttcgaggt
     1141 cgccgatgtc gaggcgagtg tgccgggcgg tccgtacgac gtcgcgtact cgcgcatggg
     1201 cacgatgttc ttcgcgagcc cggtgttcgc gctgcggaac acgcgcaaga cgctcgtgcc
     1261 gggcggccgg ctgagcatgg tcgtgtggcg caagaaggag acgaacgact gcttctatcc
     1321 agcagagctg gccgtccgcg agctgctcgg tgatccgccg aagaacgatc aggtgacgtg
     1381 cggtcccggt ccgttctcga tggcgagccc cgatctcgtg agcgaccagc tcatcggcgc
     1441 cggcttcgcg aacatcagct tcgagcgcag tgatgcgctg atgaagatcg gccgtacgat
     1501 cgaggaagcg gtcgagttcg ccctcatggt cggccccgcc ggggaagttg ttcggctcgc
     1561 cggtgacgcc gcggtttcaa ggcgcgcgga gatcgaggct gccgtgcgca aggtcgtcga
     1621 gccgtacgcg accgccgacg gcgtgatggc accgtcgtcc acgtggatcg tgaccgcgac
     1681 gaatcccgcg acgtagctac gactgcgccg agcgcgcctg gatcgcagac gcgagcgaac
     1741 gcgcgagcac gtccggcgag tacggcttcg tcacaaagcc gctcgcgccc gcatcgagca
     1801 ggccctggac gtcctcgttg accgagtagc ccgacatcag gagcacggcc acggtcggat
     1861 cgatgtcacg catcgccatg aacgtcgagc tgccgctcat gccgggcatc atcatgtcca
     1921 gcacgacggc actgatgatg tcgcggtgct cgcgaaagag gcgcagcgcc tcgtcaccgt
     1981 tcatcgcctc gaacgtgttg tagccgaggc tccgcagcgt attcgcgacg gcgcgccgca
     2041 ccaggcgatc gtcatcgacg acgaggatcg tgccggtgcc cggctcgacg gtcgccgccg
     2101 cttcgggaat cgcgggcgct tccgctggct tcttcgacgc cggcaagctg acgcgcagca
     2161 tcgcgcccgt gccgatgcca tcgccgactt cgacggtgcc gccatgactt tcggcgatgc
     2221 cgaacacggt cgcgagacca aggcccgtgc cgcgttggct gccgtgcgtc ttcgtcgtga
     2281 aatacggctc gaacacgcgt tcgcggatct cgtgcgggat gcccggcccg tcgtcgagca
     2341 cgtcgagcag cacgcgctcg cccgccgtcg acgtccgcac gatcacctga ccgccattgt
     2401 ccttcaccgc gtcacgcgcg ttgacgacca gattcatcac gacctgctcg agctgcgacg
     2461 cgtcgccgat taccgtgcct cgatcctgcg cgccgagctc gaacgagatc tggatcccgg
     2521 tcagcgtgcg cgtcagcagc tcgcgcatcg actcgaccac atcattgatc gcgactggca
     2581 tcgcacggtg cttgcccttg cgcgcaaagc cgagcaacga gcgggtcagc atcgccgatc
     2641 gctccgtcac gtcgtcgatc atcttgagcg acgcgagccg ctgcggatcg gactccgagg
     2701 cggcgagctc agccgccagc agcttgatgc cgaagatcaa attgttgaag tcatgcgcga
     2761 tgccaccggc gagcgtgccg atcgcctcga gccgctgcgc gcgacgcagc cgcgtctcgc
     2821 tctcctcgcg cgcgtgctct gcctcgacct cgcgcgtgat gtcgatgaac gcgacgatga
     2881 cgtgcgagac gacgcccgct ttctcgacgg gacgtgccga cgcacgcacg tgtcgaatcg
     2941 tccgatccgg ccgtcggatc atgatgtcat cgacgactac gacgcgcttc tcgacgagcg
     3001 cgcgcacgaa cggcagcttc tcttccggat acggtcgacc gtcgcgcgtc aggattccgt
     3061 acggctccgt gtagccaccg acgcgaacgt gcgccaaatg ctgccccatg atctcgccga
     3121 actcgtggtt cgtgtagatc tcttcgccca tcggcgcacg cgccacccat acggcgaccg
     3181 gaagatcatc ggccagcgcg ttgatgatgt cctgctcgga cggtacatcg gctttcgaca
     3241 acgctcactt ttaacacaag cgtcgacgcg gccgatacgt ctcgcatcgg tcgcaccgag
     3301 tcagcaaacg acgcgctggt acgatcagtg tgccgcgccg ttgccgttgc cgttgccgtt
     3361 ggcgctcgcg ttgccggcac caagatgtcc gagcaagttg gcgaggtcgt cggcgtgctc
     3421 ttcttccttc gcgagaatct cttcgatcat gcgacgcgtg gtcggatcgt cgttggcgag
     3481 ccaccggatg atttcggagt aggtcgcgat cgcgatgcgc tctgccacga gatcctcgcg
     3541 gatcatctcg tcgagcgtct cgccttcggc gtactcggca tggctgcgcg tcttcagtcc
     3601 gtctggattg aagttcggcc ggccattcag ctcggtgatt cgtttggcga cctgctcgac
     3661 gtgtacctct tcctcttggg cgtgctccaa gaactccgcg gccaccgact gcgcatgaat
     3721 gccgtgcgcc atgtagtagt ggttcttgta gcggagcgtg cagacgatct cggtcgccag
     3781 cacctcattg agtacctcga tcaccttctc gcggtccgcg ccgtaactcg ccgtcaccgc
     3841 tccctcttcc atcttgtcgc gggcgcgccg ccggatctct tccaggtctg ccttgaaggt
     3901 gttcatgacg tggcgcgttc caagaaccgg gcccccagca accggtcgca accctccgaa
     3961 acctccaggc gcacgacgcg cgccgatgac acacgcgaac gccgcgctcg gttacgatgg
     4021 gcgcgcagtg cttcgctggc ccggcaacat cctccacttc ttcttcctgc tgctcgtcct
     4081 cctgctcgca ggattcatgt acgggatcgc gcggcttggc atcttcctct tcgtatgggg
     4141 cccgcgccgc accaccgcgg tcggcacact acgcggctgg atcctcaaac agtcgatgag
     4201 ctcgctcggc gcgaccttca tcaagctcgg ccaagtcatg tcgacgcggc cagatctctt
     4261 cgctccggaa gtgatcgagc agctgcgctc actccaagac cggatcccgc cgttcgcctt
     4321 tcgcaaggtg aaagcggtta tcgaatccga tttcggcaaa ccgctgcacg acgtcttcag
     4381 cgaattcgac gaaaagccgg tcgccgccgc gtccgtcgcc caagtccacc gcgctcgcat
     4441 caaaggcgga gacgaagtcg ccgtcaaaat cctgcgccca ggcgtccgcc gccaagtcga
     4501 gcgcgaccaa gcgatcctca tcctcttcgc ccgcgtcctc gccctccgcc cgacgtggcg
     4561 ccgctccgac cccgtcggcc acacgcgcca cttcatcgaa gcaatccacg atcaaacaga
     4621 cctcggcatc gagcgcgaca actaccgccg cttccacgcc aacttcgcaa actccccgac
     4681 cgtccgcttc cccaaagtct acgaagacct gtgcacgccg cgcgtgctca cgatggagtt
     4741 catccgcggc gtcaaggtcg acgccctgcc gccgggcaaa tacccgaagc tcgccgaaac
     4801 cgtccgcatg acgttcttca agatgtgctt cgaccacggc ttcgtccacg cagacctcca
     4861 ccccggcaac atggtggtcc aagacgaaac caagggcgag ctcgtcatct acgacgtcgg
     4921 cctcgccaag ctcctgcacg aagacgtcct catccaattc atcgacatga cgaagtgcat
     4981 cgcgatgggc acgcccgacg acatcgttgc ccacctcaag cgcttccaca catacatcgg
     5041 cgaaatcgac tgggccgccc tccgcgtcga agtcacggcc ttcgccgaaa acttccgcgg
     5101 caaggacacc gccaagctcg aatacggcga gctcatcggc gacatgttcg cgatcggccg
     5161 caagcacaag gtccgcccgg tcaccgacat gatgctcgtc ttcgtcgcgc tcatcaccgc
     5221 ccaaggcatc ggcaagatgc tcgaacccga gcacaacgtg ttcggcgcgg tcgcccaatt
     5281 cctgatcccg atcctgatga agcgcaacga gtcggtgccc gaaacgcccg aagccaaagc
     5341 ggcgcgcgcg accgcctaac caacccgcgc tccccccaat ccgcgctctc cccagcgctt
     5401 cgcacgtcac gcttgccgcg cccgccgcgg tcgtggcgcc gaaccgtagc gctgcgtgcg
     5461 tcggtcgtct gcaacgctgc agcgaaccgc cctcgtcctc gatgccgctc agagcgccgg
     5521 ccggcgggaa gccgcgcgcg cggccctctc aacgggccgc cagcgc
//
LOCUS       DATLBZ010000222         5249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9161766, whole genome shotgun sequence.
ACCESSION   DATLBZ010000222 DATLBZ010000000
VERSION     DATLBZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9161766"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VMZ53_11745"
     CDS             <1..195
                     /locus_tag="VMZ53_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS29182.1"
                     /translation="LLADNVQMVFDGVRVTRVDPGTGSGDDQLDPPGDESSGCNARGG
                     SGTGAGLLLALGLVLRRRRR"
     gene            complement(199..375)
                     /locus_tag="VMZ53_11750"
     CDS             complement(199..375)
                     /locus_tag="VMZ53_11750"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HUS29183.1"
                     /translation="MEDTVRLRCPYCREWVDLYVDPDTQGTLVEDCAVCCRPWAVSVY
                     RDGDRLRVQVSRAQ"
     gene            complement(378..1466)
                     /locus_tag="VMZ53_11755"
     CDS             complement(378..1466)
                     /locus_tag="VMZ53_11755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS29184.1"
                     /translation="MPDGPTGQSAPVPGVLVVWTGAAPTVQVYRVPAAGLVIGRELLQ
                     TTTDDRISRQHARIMWKDRRFVVTDLGSRNGTYAGGHALIDREVTVTPPSVVRTGRTV
                     SVLIEDVRRFESGAIETKHDAIVGASTHALWTNMEQAANAGTNLLIIGEPGSGKGRMA
                     RGYARVRGRPEKVFNPTIQAVPLDRVITEQIETLVLEQVGKFGAKNTETLGKLLAERP
                     NLRVCTTAVLPLEHLGMPPDIAKRLSQAVIHMPPLRDRPDEMAFLVHDAVRSSEPGLT
                     IHSTLIEACLLRPWPGNSRELVSEVSRTAHTVASQGKNNVRGEDLDNDAGHLMVGAPT
                     LNAAVTPTAVGKQRRKRPTSRPPSSRGD"
     gene            1530..2195
                     /locus_tag="VMZ53_11760"
     CDS             1530..2195
                     /locus_tag="VMZ53_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUS29185.1"
                     /translation="MTFDAGLTLIELDLDFLAQRLGEREIAVDVAALVASAPSAWLHY
                     DALAPTSTHPWQDFMAKLLEGAGVPNVGPVVDWLWSQQPHKNLFRKPIAPMVELARDL
                     SRRGITVAVLSNSEGRLRELLTEIGVADPFAAIIDSGLLEFAKPDPRIFAHTLAELGT
                     PDAVPIHIGDSWTADIAAALAVGWRAIWYRSRGGIATGNASVPIAQNADEVRRALRDY
                     GVL"
     gene            2205..2885
                     /locus_tag="VMZ53_11765"
     CDS             2205..2885
                     /locus_tag="VMZ53_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29186.1"
                     /translation="MPLALRAWLRYLVPLTLLAAIACGPLLYTALHAHVPKDIPSARM
                     QVRIPWLLASFAWISQLWLVAAAAPLVRSVDMAEPLSQRRALWVGLCGLVRGALPVAA
                     AVIAILLGGLALAIPGVLLLALLSLTGASDALREPLPAPLLDSISAVRASWRRVVLTL
                     AVIIVVALVITFVVQKLYVPAIKSKVPPAKMLPIRTFVRVVGISLFALSPLAACALAA
                     CYRSQRSR"
     gene            complement(2861..4480)
                     /locus_tag="VMZ53_11770"
     CDS             complement(2861..4480)
                     /locus_tag="VMZ53_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS29187.1"
                     /translation="MARKPDFDLAAELAKPSFTPAVKDAPALVDLVIEGHERAGDALA
                     TLGATARTAIEQRLTPELVARDRAALISVLGQLARKDDATARDIVIAQLSIADSQVRK
                     AAVQALGKIAGEEATRVLLGRWDASDVTPDERRALAEALGKVGGEAALSRLRALDPGD
                     DAELARRRDRALLMAGRDAKRSEDSYVRVDVAPTWALDLRLRCKSGLAPLLREELGAL
                     GIRAKVPLDDSADIVLEKPWSALFASRLWASAAIRVPLAAIAAKPAAKSKGEAPSDDP
                     RAIVDVLTSDRVRELFRAWTSGPIRWRLGFAEGKQRAAIWRVAKDVTARAPELVNDPS
                     QTTWDVRIDDDSHGDRVLEISPKRYDDPRFAWRVAEVPAASHPSVAAALAYVAADGAA
                     KSGVRVWDPFCGSGAELVERSVRGGVAALVGTDLDDAALDAAKRNIDAAKLTATLAKA
                     DARSHDPGSVDLVITNPPLGSRVHVDAGKLLVDALPNFVKRLAPGGRLVWITPQTKKT
                     SPVAERLGLRLAKRYAVDLGGVRGHLERWDR"
     gene            complement(4489..>5249)
                     /locus_tag="VMZ53_11775"
     CDS             complement(4489..>5249)
                     /locus_tag="VMZ53_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29188.1"
                     /translation="KRVGDGSGVVIVSWEPGGNLTRDELRAMLPIFTKAMSVADAGGT
                     TKLGSVVGPDGKPLDTITFDTDSLDLELSMLPCGVRHVMIATAATHGVMPLHQRIVGS
                     FACHPDAALEATATSLAFPLALDLPGWYVVGRDEEVTQISSDTASLVLRTLPPSLKTP
                     DLATFIGPAFEQTGAHVEIIEKRGGDFIKVRLEDQGDSGMGWVALFRCPTGIALVLGL
                     GHDDATVTALYEKVKGSGCLAAGEKPTQFAESPN"
BASE COUNT          784 a         1848 c         1724 g          893 t
ORIGIN      
        1 ctcctcgcgg acaacgtgca gatggtgttc gacggcgtgc gcgtgacgcg cgtcgatccg
       61 ggcacgggct cgggtgacga ccagctcgac ccgccgggtg acgagtcgtc cggttgcaac
      121 gcacgcggtg gctcaggcac cggtgcaggc ttgctcctcg cgctcggcct cgtgctgcgc
      181 cgccgccgcc gctagaaatc actgcgcgcg cgagacttgg acgcgaagac gatcgccgtc
      241 gcggtagacg ctcacggccc atggccggca gcagaccgca caatcttcta cgagcgtgcc
      301 ctgcgtatcg ggatcgacgt agagatcgac ccactcgcgg cagtacgggc agcgaagtcg
      361 gacggtgtct tccacagtta atcgccgcgc gagctgggcg ggcgcgacgt cgggcgcttg
      421 cggcgctgct tgccgacggc ggtcggtgtg acggcagcgt tgagcgtcgg cgcgccgacc
      481 atcaggtgac cggcgtcgtt gtcgaggtcc tcaccgcgca cgttgttctt gccctgcgat
      541 gcgacggtgt gcgcggtgcg gctgacttcc gagacgagct cgcgcgagtt accgggccat
      601 gggcggagta ggcacgcctc gataagcgtc gagtgaatcg tcaggccggg ctccgacgag
      661 cgcacagcgt cgtgaacgag gaacgccatc tcgtcgggac ggtcgcgcag cggcggcatg
      721 tgaatgacgg cttgcgaaag gcgcttcgcg atatccggcg gcatgccgag gtgctcgagc
      781 ggtaggaccg cggtcgtgca gacgcgtagg ttcggtcgct ctgcgagcag cttgccgagc
      841 gtctcggtgt tctttgcgcc gaacttgccg acttgctcga gcacgagtgt ctcgatttgc
      901 tccgtgatca cgcgatcgag cggcaccgct tgaatcgtcg ggttgaaaac cttctccggg
      961 cgaccgcgca cacgcgcgta gcctcgcgcc atgcggccct tgccgctgcc gggctcgccg
     1021 atgatcagga ggttcgtgcc ggcgttcgcg gcctgctcca tgttcgtcca gagcgcgtgc
     1081 gtgctcgcgc cgacgatcgc gtcgtgcttc gtctcgatcg caccgctctc gaatcgccga
     1141 acgtcttcga tgaggacaga caccgtgcgg cccgtgcgaa cgacggaagg cggcgtcacc
     1201 gtgacctcgc gatcgatcag cgcatgacca ccggcgtacg tgccgttgcg actgcccaga
     1261 tccgtcacga cgaaccgacg gtccttccac atgatccggg cgtgctggcg gctgattcga
     1321 tcgtcggtcg tggtctgcag gagctcgcga ccgatcacca gaccggcggc tgggacgcga
     1381 tagacctgga cggtcggcgc agcgccagtc cacacgacca acacgccggg aacaggcgct
     1441 gattggccag tcgggccatc cggcatcacc taccgagtat acccctccgg cgggttcgag
     1501 gtatcaccac acctatgccc aagcccgtcg tgacgttcga cgcgggcctg acgctgatcg
     1561 agctagacct tgacttcctg gctcagcggt taggcgagcg agagattgcc gtcgacgttg
     1621 ccgccctcgt ggcctcggcg ccatccgcgt ggcttcacta cgatgcgctc gcgcctacga
     1681 gcacccaccc ctggcaagac ttcatggcga agctgctgga aggagcgggc gtgccaaatg
     1741 taggcccggt cgtcgactgg ctctggtccc agcagcccca caagaatctc ttccgaaagc
     1801 ccatcgcccc gatggtcgag ctggcccgcg atctcagccg ccgcgggatc acggtggccg
     1861 tcctctcgaa ttccgagggc cgcctgcgcg agctgctcac agaaatcggt gtggccgatc
     1921 cgttcgcagc gatcatcgat tcgggcctgc tcgaatttgc caagccggac ccgcgaatct
     1981 tcgcgcacac attggcagag ctcggcacgc ccgacgcggt gccgatccac atcggcgact
     2041 cgtggacggc cgacatcgca gccgcgctcg cagtgggctg gcgcgcgatc tggtatcggt
     2101 cgcgtggcgg catcgcgacg ggcaacgcgt ccgtgccgat cgcgcagaat gcggatgagg
     2161 tgcggcgagc actccgcgac tacggcgtgc tttagtatcg cgtcgtgccg ctcgcgctcc
     2221 gcgcctggct tcgctacctc gtgccgctca cgctgctcgc agcgatcgcg tgcgggccgc
     2281 tgctctacac cgcgcttcat gcccacgtgc cgaaggacat cccgagcgcg cgcatgcagg
     2341 tgcgcatccc gtggctcctc gcgagcttcg cctggatctc gcagctctgg cttgtcgcgg
     2401 cggccgctcc gctcgttcgc tccgtcgaca tggccgagcc gctctcgcag cggcgcgcgt
     2461 tgtgggtcgg tttgtgcggt ctcgtgcgag gcgcgctgcc cgtcgcggcc gccgtcatcg
     2521 cgattctcct cggcggtctc gcactcgcga ttcccggcgt gctcctgctc gccttgctgt
     2581 cgctcaccgg cgcaagcgac gcgctccgcg agccgctgcc cgcaccactg ctcgacagca
     2641 tctccgccgt tcgcgcgagc tggcgccgcg tcgtgctcac gcttgccgtg atcatcgtcg
     2701 tcgcgctcgt gatcacgttc gtcgtgcaga agctctacgt gccggcgatc aagtccaagg
     2761 tgccgcccgc gaagatgcta ccgattcgaa cgttcgtgcg cgtcgtcggc atctcgctgt
     2821 tcgcgctctc gccgctcgcc gcctgcgcac tcgccgcttg ctatcgatcc cagcgctcga
     2881 gatgaccgcg cacgccgccg aggtcgacgg catagcgctt cgcgaggcgc agcccgagtc
     2941 gctccgcgac cggcgacgtc ttctttgtct gcggcgtgat ccacacgagc cggccgcccg
     3001 gcgcgagccg cttcacgaag ttcggcagcg cgtcgacgag cagcttgccc gcatcgacat
     3061 gcacgcgact tcccagcggc ggattcgtga tcacgagatc cacgctcccc ggatcatgcg
     3121 agcgcgcatc tgccttcgcg agcgtcgccg tgagcttcgc tgcgtcgatg ttgcgcttcg
     3181 ccgcgtcgag cgccgcatcg tccaagtcgg tgccgacgag cgccgcgacg ccgcctcgaa
     3241 cgctgcgctc cacgagctcc gcacccgagc cacagaacgg atcccacacg cgaacgccgc
     3301 tcttcgccgc gccatccgcc gccacatacg cgagcgctgc cgcaacgctc ggatgcgacg
     3361 ccgccggcac ttccgccacg cgccacgcaa agcgtggatc gtcgtatcgc tttggcgaga
     3421 tctcgagcac gcggtcacca tgactgtcgt cgtcgatgcg gacgtcccac gtggtctgcg
     3481 acggatcgtt gacgagctcg ggcgcgcgcg ccgtcacgtc tttcgcaacg cgccagatcg
     3541 ccgcgcgctg cttgccctcg gcaaaaccca ggcgccaccg aatcgggccg ctggtccacg
     3601 cgcggaatag ctcgcgcacg cgatcgctcg tgagcacatc gacaatcgca cgcggatcat
     3661 cgctcggagc ctcgcccttc gatttcgcgg cgggcttcgc agcgattgca gccagcggaa
     3721 cgcgaatcgc cgcgctcgcc cacagcctcg acgcgaatag cgcgctccac ggcttctcga
     3781 gcacgatatc cgccgaatca tcgagcggca ccttcgcgcg gatgccgagc gcaccgagct
     3841 cttcgcgcaa cagcggcgcg agccccgact tgcatcgcaa ccgaagatcg agcgcccacg
     3901 tgggcgcaac atcgacgcgc acgtatgaat cctcgctgcg cttcgcgtcg cggcccgcca
     3961 tgagcaatgc gcgatcgcgg cgtcgcgcga gctccgcatc gtcgcccgga tcgagcgcgc
     4021 gcaaccgcga cagcgctgcc tcgccgccga ctttgccgag cgcctccgcg agggctcgcc
     4081 gctcgtccgg cgtaacatcg gaggcgtccc agcgccccag cagcactcga gtcgcttcct
     4141 ctcccgcgat ctttccgagc gcttgaactg ccgccttgcg tacctgcgaa tcagcgatgc
     4201 tgagctgcgc gatgacgatg tcgcgtgcag tcgcgtcgtc tttgcgcgca agctgcccga
     4261 gcacggagat cagcgcggcg cgatcacgcg ccaccagctc gggcgtcaac cgctgctcga
     4321 tcgcggttcg cgcggtcgcg cctaacgtcg ctagcgcatc gccagcgcgt tcgtggccct
     4381 cgatgacgag gtcgacgagc gcgggcgcat ctttgaccgc gggcgtgaag ctcggcttcg
     4441 ccagctcggc ggcgagatcg aagtctggtt tgcgcgccat gggagccgtc agttcgggga
     4501 ttcggcgaac tgcgtcggct tctcgcccgc cgcgaggcaa cccgagcctt tcaccttctc
     4561 gtagagcgcg gtgactgtcg cgtcgtcgtg gccgaggccg agcacgagcg cgatgcccgt
     4621 cgggcagcgg aacagcgcga cccatcccat gcccgaatca ccttggtctt cgagcctcac
     4681 cttgatgaag tcgccaccgc gcttctcgat gatctcgaca tgcgcgccgg tttgttcgaa
     4741 cgcggggccg atgaacgtcg cgaggtccgg cgtcttcagc gacggtggca gcgtccgcag
     4801 gacgagcgac gcggtgtctg acgagatttg cgtgacctct tcgtcgcgac cgacgacgta
     4861 ccaaccggga aggtcgagcg cgagcgggaa cgcgagtgag gtggccgtgg cttcgagcgc
     4921 cgcgtcgggg tgacacgcga agctcccgac gatgcgttgg tggagcggca tcacgccgtg
     4981 cgtcgcggcc gttgcgatca tcacgtggcg cacgccgcac ggaagcatcg agagctcgag
     5041 gtcgagcgag tcggtgtcga acgtgatggt gtcgagcggc ttgccatcgg gaccgacgac
     5101 gctgccgagc ttggtcgtgc cgcccgcgtc agcgaccgac atcgccttcg tgaagatggg
     5161 cagcatcgcg cgcagctcgt cacgcgtgag attgccgccc ggttcccacg acacgatgac
     5221 gacgcccgag ccgtcgccga cgcgcttca
//
LOCUS       DATLBZ010000223        25960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_225007, whole genome shotgun sequence.
ACCESSION   DATLBZ010000223 DATLBZ010000000
VERSION     DATLBZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 25960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25960
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225007"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VMZ53_11780"
     CDS             <1..974
                     /locus_tag="VMZ53_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29189.1"
                     /translation="TRFRNAPIDPFREPPFGVLWHRTTLAFRCHHAAADGELFIEIVR
                     HFLAALSADVPTSTKPIDHPLALRHLRKQATVRNSLRYARWLATEAKSNRSLRLHLAR
                     SAPGPIAICERVLSTADRDRLFSHAREERVRPAWLIAAAWMRALHAFSVTTNDATTPL
                     VSLEVPVTLRRGSDIGGNALAVTTLFGDARRPLADLARGLWRDYAKHIKQRDHLAVPL
                     LSAPIRLLPWPLFKRVAVTTTSTGFATSHFTWLDHAPEGLSIERRHLYTPVCQHMGAA
                     LCALASRDSLQLSITHRTNGISSAAASSLADHLLEALTSRAVTSAAS"
     gene            complement(958..1383)
                     /locus_tag="VMZ53_11785"
     CDS             complement(958..1383)
                     /locus_tag="VMZ53_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS29190.1"
                     /translation="MFVKNVVGVLVAMAIITTLALSWGRPSNHPQPKYKPLPTISKAM
                     PPEEVGEIAFQKMGCATCHTIDGSTRIGPTFLHDFGSRITLDTGEMIVVDDAYLRESI
                     EQPRAKSRPGYPPAMPAEYATLLTDLEKEGLVAYIKTLR"
     gene            complement(1436..1912)
                     /locus_tag="VMZ53_11790"
     CDS             complement(1436..1912)
                     /locus_tag="VMZ53_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29191.1"
                     /translation="MNWGSWLIWGFASTVVLTTLMAGSQGLGITRMNIPYLLGTMVTP
                     DRDRAKLLGIGVHLVNGWLFALIYIAVFHASGGPTWWKGAAIGAVHSMFVLVVAMPAL
                     PGMHPRMASETRGPTVTRLLEPPGFLALHYGTRTPLSVVASHLVFGALLGLLYRPL"
     gene            complement(1909..3741)
                     /locus_tag="VMZ53_11795"
     CDS             complement(1909..3741)
                     /locus_tag="VMZ53_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HUS29192.1"
                     /translation="MKRAVSVDDYGIIGDGRSAALVGRDGCIDWLCWPRFDSPPIFGA
                     LLDSRAGFWSIAPVEPGRVSRRYVERTNVLETRFDTATGSVVLTDSMSIASEDVKHKE
                     LVPEHELLRRVECLSGFVELEVVVCVQPDFGRFRARTLDLGALGIRWEIGSQLLTLRS
                     DRPLMLGDDGRVRARVRITAGKALHWSLSFDAEAPAVLPPLGAAASERIARSVEWWRQ
                     WVGRTRYNGPHRDAVLRSVLVLKLMSFAPSGAIIAAPTTSLPEVPGGEHNWDYRYCWL
                     RDASFTARVFLQLGFIEDAQAFASWLLHATRLTQPALRVLYDVYGNQPPPEHIVPLEG
                     FGGSQPVRVGNGADRQLQLDLHGEVIDACAQVIETTGHIDQETRELLRGFGEHVIENW
                     REPDYGIWETRTAPVHHTHSRLLSWVALDRLDRLADAGHLQLDRARIADERTAIRRQI
                     EDRAWHRGLQSYTAELDGDQLDAAVLLMSWYGFHPANSARMRSTYARLTAELSPMPGL
                     LYRRESSNQIGEGAFWLCSYWAVEHLIRGGGSLEQALTLFGDATSYANDLGLMAEEVD
                     VHTGRALGNFPQAYTHVGLISAALSIADRLPVARALPRRAEARP"
     gene            complement(3738..4757)
                     /locus_tag="VMZ53_11800"
     CDS             complement(3738..4757)
                     /locus_tag="VMZ53_11800"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS29193.1"
                     /translation="MDDNDPWIPEGALAGKVAVITGASAGVGRAIARAFSAAGAKVAL
                     IARDHVGLAATADELGISLVCPLDVADAAEVDRVADAVVGRWGQIDIWVNNAMESVFA
                     PVDQITAAEYRRVMEVNYLGYVHGTLAALRHMKKRNRGVIMQIGSALAYRSIPLQSAY
                     CASKSAIRAFSESLRCELLHEKSGIQVTQLHLPAVNTPQFEVVRNRLPGHPQPMGQIH
                     QPELIARTAVRAALKPKRELWIGWPTVKAILGQRVLPGWLDKKLARDAWEGQTTDDVP
                     VGHRGKHRDNLYLPIERDFGAHGIFDDRAQDSSITEWWREHKGAVVVASAAASAIAAL
                     AWRWR"
     gene            4898..6133
                     /locus_tag="VMZ53_11805"
     CDS             4898..6133
                     /locus_tag="VMZ53_11805"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HUS29194.1"
                     /translation="MTFGRNSLLEVGDRARALGMRRVALVTDRQLRALPWFDDVSRSL
                     ATAGCEIIIFDDVSIEPTDASFEAAARMASDAKPDGYVSLGGGSVIDTAKAANLYATY
                     PAALRAYVNAPLGDARAIPGPLAPHIACPTTSGTGSEVTGIAIFDWREHHAKTGIASP
                     RLRPTEAIVDPRTTETLPATVVAASGMDVLCHALESFTARAFSHRTSPKPATARPMSQ
                     GSNPWSDLGCREAMRLAATYLERAVLDAADSEAREQIMWAATLAGIAFGNAGVHVPHA
                     MAYAVAGRVRDFAPAGYPATPIVPHGMAVSVGAPSVFRAFGTFCSDRHLEAAALLGAD
                     TRGASPSDAGALLADRVIAISQSIGIPNGMSGVGYTRSDIADLVAGTLPQQRLLSNAP
                     CDVDAECLHALFEGALAYW"
     gene            6137..6568
                     /locus_tag="VMZ53_11810"
     CDS             6137..6568
                     /locus_tag="VMZ53_11810"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HUS29195.1"
                     /translation="MPAKERPTIDTYPYATAITTRWMDNDIYGHINNVTFYSFFDTAA
                     NKFLIDRGGLDIANDPVIALVVESNCSYHAPLAYPANIRAGVRVAHLGNRAVTYDIAI
                     FSDRDPAAAANGKFVHVFVDRATRQPTMIPAAIRKALQTLQ"
     gene            6565..7029
                     /locus_tag="VMZ53_11815"
     CDS             6565..7029
                     /locus_tag="VMZ53_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS29196.1"
                     /translation="MTLEVRPVEARDVPEVLELVAETLAEFGLEFGKGSPTDDQLRQL
                     PASYSDHGGAFWVALRDGELLGTAGVFPVEDRMYELRKMYLRPASRGLGVGKRLLHAA
                     VDWVRARGGTHIVLDTIEEMTRAIAFYEANGFVRDDAQKRAARCTRGYARKL"
     gene            7067..8461
                     /locus_tag="VMZ53_11820"
     CDS             7067..8461
                     /locus_tag="VMZ53_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29197.1"
                     /translation="MIEGSTIGQYRVIRKIGEGGMGAVYEAQHTLIGRRAAIKVLLPE
                     LSSHREIVDRFFNEARATTSISDPGIVQVFDFGVHTDNSAFIVMEFLEGEPVDSRMKR
                     FGRLTPFDALRITRQCAGSLAAAHQRGIVHRDLKPENIFLVKDQEAQGGERAKILDFG
                     IAKLSNNESSTKTRTGMMMGTPVYMSPEQCRGAGSVDHRSDIYSMGCVLYHMLTGRPP
                     FDAEGMGELIAMHLREQPKPPSELVQGINIVDEVVLTCLAKDPAQRLQTMTDLQRACD
                     AVMARITNSGASAQTVALATPVPPGFRSEFPQAAAAPLQPATQVLGKPTTLSAATGEA
                     GTTPPTPRRRVGLFAGIGVLVAGGIVAAVVMAGGSKPKPESGTAATPGSNAAPVTAVP
                     ADAPAVVTAVPPDAAPVAPPDAAEIATSPPDAGTTAAAVVTTPVKDPKRPKKPTTTKP
                     NTGSASGGNLYDNR"
     gene            8448..9449
                     /locus_tag="VMZ53_11825"
     CDS             8448..9449
                     /locus_tag="VMZ53_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29198.1"
                     /translation="MTIAKLALAALVLLVAVRPALAQSVEAEALFREGKELLKQGKLG
                     EACAKFDASDRIEPSVGTELNAADCHEKNNKLATAWAGFLKAAGNAAKAGNDKQREAE
                     AKRRASLLEPKLSYLTISVSESSRVTGLSIKRNGVDVDQALWNTGIPVDAGDYEIAGQ
                     APGHEAWSTKVTVAVSERKSVEVPRFKEITALVTPPPDQNPNPLEKEPVEQPHETPST
                     FTPMRKAAIGAAAVGVIGIAGTVVFGLKANSKQSEAEDLCPAAPACADPMAIQLNDDA
                     RSAAKLSNVMLGVGVVGVGAAVALWFIGAPKEGTPNDVAIVPVTNGRDVGLSFSGSF"
     gene            9453..11528
                     /locus_tag="VMZ53_11830"
     CDS             9453..11528
                     /locus_tag="VMZ53_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29199.1"
                     /translation="MRVSLYLALAFVAGCSVPDFFLVDAGHGGDDGGADAKPDAALDA
                     PPGAATISFVSSSNTDGYYKAADTISIQVTFSEAVTVDTTGGTPTLTLNSGASASYAS
                     GSGTTILTFSYTVAAGNAAADLEYTTATALSANGGTIKNGSVNAFLVLPVPGAAGSLG
                     ANKAIVIDAVAPTISAVTGPNAYAPSASATLSYAVTDANPGTTTCTQVTGTGTMASCT
                     ATGVTFSGLNQGAHTISITHTDAAGNISAAQTYSWTVDTTAPVVSTISGPALFAPSTS
                     ASLTYSVTETNQGTTTCTFTTGSGTITSCTASGASVTGMNQGAHTISVTHTDLATNAS
                     TAQTYSWTVDTVDPVISITGPTNNWTAGNNVSLTVSITDANPSTTSCAHPVGTGTQTN
                     CSPTALTYTGLSTGAHEVHVIHTDRAGNTTDQTYTFSVCPPVSFATPSIPNFQVPTGC
                     GTVMDVQAWGAGGAGGASNGGAGGGGGYARGTLTVAAGQSFIIRVGGGGTRSTTGAGG
                     RGFNDGGSAGLNGGGGGGWSGVNFGNGSDMLRAPGGGGGGGCNGAAAGGGGGGLNGSP
                     GGGSLPGGGASQGAAGVGGGTGSSGGVGQGGSGGDSASGCSGGGGGGGRFGGGGGNGP
                     NTGPSSGAGGGGSVFLDSSLASTMTQAGTGVNAGGVAEPNYINGRGFGGSSNGGTGGD
                     GLVVLHVHP"
     gene            complement(11554..13644)
                     /locus_tag="VMZ53_11835"
     CDS             complement(11554..13644)
                     /locus_tag="VMZ53_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding EGF-like domain-containing
                     protein"
                     /protein_id="HUS29200.1"
                     /translation="MKRFTRGGTMQFTKIGLLALALAACGDDSGNGPSDAAVVVIPDA
                     PEFAPVITGLAASPASGLSPNVPTAVTFNWAYLVEPTYPEPTCTIDNGVGTVMRGVPV
                     MITISTLTLFTMTCSNRAGQGMRQVIVNVTPSAPQIATFTVTPSSVTPGTATPLTFTW
                     TYSNTPSPVPTCSIDNGQTTITSGTPTTLTLTQARTFRLRCTNGVGGPVYSGGAIADV
                     TVAANECGTAYAQCDAHAACTDTTNGYTCACGNGYNGDGDTCSLTTTVSTDCDSNAQF
                     LGTSCVCKAGYFGSGAVGDCKRGRFTFVTSVTGSGLLSSWLGAGTNNGLAAADAICQA
                     RAAAHTSTAYPSGLPGTYVAWISDSTHDAYCRAQTDGATGHLNGKKAGNCGLGQLPIA
                     AGPWVRVGDWKQWSPAIDKLLTPNSITYFPANRDENGNEITSTTDRVWTGTDNSGQYV
                     AGATCNDWLSSSSSFAGVAGEVHGGGSSWTQTSATNPFCNNTTGFHLRCVEVSATTPA
                     ALPPRHPTLVKKAFITSVQGNGLLSSWPDSYGQTGSNAPDAICQARARYAGFANAAAF
                     KAWLAYPSYISNKFTSTTFTYVRPDGVPLGTSRNDMLDSKLSAAWNQTELGTYLTGNG
                     DTGSVTTNIYGASGTTGQYYGSSSAVCSNWSSNASFYNWYIGRYDLLDSRAYNLQTTS
                     TCDQTNIRFYCVED"
     gene            complement(13641..14720)
                     /locus_tag="VMZ53_11840"
     CDS             complement(13641..14720)
                     /locus_tag="VMZ53_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29201.1"
                     /translation="MRLALISAFGLLAAACGGDSSVDISTNQDNFCEQIADVVCHNLY
                     QCCTESEIEDRLSVSEPRTEAQCRQDYSRLCQRGGADVRDSLQDGRITFDPAKLNGCL
                     EAILAPTDTCSAVAEATVPWKEACKDAPWVGTVAVGAACLFPFDCAGAPDAFCGPDLK
                     CKMKPTAGFPCSTSNPCASAYYCQPTTSTCAPKVAAGAACTSSTQCGTDLFCDNKNTI
                     DPADDVCAPKGAGGAACHQAASSQFTSQALANAACISGTCNPGKCSGTSQNCYSDLNC
                     GGRCSLSPTTSCVNSYPDCNPSGGQCTGNGQTCTSDATCIAASAGTCFFTQSCVPSQC
                     VGDVVCTSTTITADYCTAVGSIPSP"
     gene            complement(14741..15445)
                     /locus_tag="VMZ53_11845"
     CDS             complement(14741..15445)
                     /locus_tag="VMZ53_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS29202.1"
                     /translation="MGVVFASATALAQPGPLPSADFAKQGTDLYRAGDYAGAVAPLEK
                     AVELEPNNFEYRFMLAQSYRQSGHCDKAVPLYRALVDVAPDAQRKTDVETNMALCPAT
                     SVAAPPSQPAPAPEPPEPPAPAVVTSSSGGSISGANMGLLMGAGVGIGAGVVLLLSGY
                     NDSKDADRAAKFSDHDAIDTRASTEYVLGAVGVGVGVALGVVAVVRIKNAKEHDTSIS
                     FKPRSGGGTFVWERTW"
     gene            15620..16561
                     /locus_tag="VMZ53_11850"
     CDS             15620..16561
                     /locus_tag="VMZ53_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29203.1"
                     /translation="MRRLLPALAISIPLVSLVFARHLSATTSWLTPFEIAISIALGIA
                     SLTTIVALFAGWKRVSIAVPVALLSINVVFMLARAADVKLGTLPPPDVKFAIVLSAGM
                     LLSMVGLVMRRPWGRWLGMAFGAAAIGCGALNLVWYWPASGTPNVSYPEWYADVCRTT
                     CFHMVTCLGGAVILINTIAASDIFHTSATWSRREGVIRWLRASMVATFVAVPMLLVYA
                     WMQPLAASTKPTALALAAVLAVGAFLGVRGKLVGALLLVLGGAGLAVQTAMTMSLTHG
                     TTRSISYYYAAFWTPCALILLITGCVLARPTLRLLRG"
     gene            16689..16988
                     /locus_tag="VMZ53_11855"
     CDS             16689..16988
                     /locus_tag="VMZ53_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29204.1"
                     /translation="MSPDDDDLPSIDPAALDTVTGGTASNDDVTAAITSLSNDLKDFV
                     SSQKNQGSNTFQQMLPFLLMMSGGNWSFSSSGGFNCSGGGGGCGRCGRRGCAGNC"
     gene            complement(16989..17735)
                     /locus_tag="VMZ53_11860"
     CDS             complement(16989..17735)
                     /locus_tag="VMZ53_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29205.1"
                     /translation="MKLSVRTFAVLAGFIIVGCGGEEKPVSNDARPRPDAPPSCPPAA
                     QPLATGTFKLFVSFEGVTVKLGDCDDSRTNCSALVTQASTVVPSFLSGDAARASHIST
                     IVGMAQDALAPFSVDVVTTRPASGNYWMVSVGGTSDAITGVADKLLAVKPVCDAMNKN
                     GIAFVFEQDATLDTPDRAYADTIAGAFGHLVGLVPTRSARDCMCVSSTCAHTQACTWG
                     TATLPEAGNSCDRTMQNEQLLLMDSIGCRS"
     gene            complement(17829..18326)
                     /locus_tag="VMZ53_11865"
     CDS             complement(17829..18326)
                     /locus_tag="VMZ53_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29206.1"
                     /translation="MRSFAWLLVVVGGCVASIDGGVLQQPTLSPKGGSVTGHVGIGGG
                     GVSTSPYMLAIDLDTRVDVASGGSRWTAGASALGGIKIVPSFFLDARIGIWRAIVSGA
                     PEASAVPSFELGGFVPLRERFDKKHPEHGADSSGVVFGVREDLDELNYFTVFVGYAIF
                     ILPGY"
     regulatory      18554..18629
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18871..20649
                     /locus_tag="VMZ53_11870"
     CDS             18871..20649
                     /locus_tag="VMZ53_11870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29207.1"
                     /translation="MATEDDERGTVVDRPTARESKPHIESARDAATAILSSAPGGTGA
                     PGSSLAQADVPSRSSSSSLGGADSLGASMSTIATARGALDHSEVVRTRNFCLVGMTIA
                     AAGLAAIPLLPGGYFTTRLFMVCIGVCIACLVYILYRTRTPETFHAGSAISIAWFIPV
                     VGVTSAIPFFGAMSPAPILLVLGIFFNGLGTTRGVGVATYVICALVQGVTGFLVAFGV
                     MADPGFIHPVHLAREYEVTCQILVQLILAGTFVVARASRRSHLAALSDLEVAVRAIAQ
                     RQALLEEARAELRRAMGSTRGRFTDQVIGVYQLGDLIGHGAMGEVYQAVDTRDNQAVA
                     IKMLSRASLGNTQHVERFMRELRTTTAITSPHVVHVLAVGEHPLPHLVMERLRGRDLS
                     AILKSRGTLNTKATIELVRQVAEGLAAAASAGIVHRDIKPQNLFLADGTWKILDFGVA
                     RVAASTDTLTQGHVIGTPAYMAPEQARGAQVDHRADIYALAAVIYRALTGHSLFKGGE
                     VADMLYKVVHTAPRKPSGLASVPTDLDFALAIGLAKKPDDRFATAGELADAVEAALTG
                     KLSAAMRERAEKITNAWESEEASPRR"
     gene            20692..22569
                     /locus_tag="VMZ53_11875"
     CDS             20692..22569
                     /locus_tag="VMZ53_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HUS29208.1"
                     /translation="MAALAETADTLRSTGIAELLAAHPWPREFADARRLEWLWTVDVS
                     VTPEVLWPLLADLSRLNRALKLPQMTFVEKEGVRWGTARYTGVKHEWQEVPWNWVAGR
                     WYELVRIYRKGSMRALHGIYDLQPTPTGTRVSIYYGVVPSSRIFDLGLEFNFRAMGRA
                     YKRLLPRIAEEAKREPHAPPCLRAPSPKLQPDAQTRLDQLAKSLEDQGLDQKLVRQLV
                     DWLGSATDEELERIRVRERARVWSIDEDELLRLFLHATRTGLLDLSWDVVCPHCRGVR
                     DATGQLDSLPTSGQCDACGIAFGTDSPDAVEITFHAHSSIRPVERGVYCSAEPVHKPH
                     IHIQHTVQPGERATVTVPVGAGKYRMRVRGEMRGAWLEIADGAPTTLAWSSADLPETA
                     KLSSKGTLELTNTGDKPATFVVETAVWLELALRPGRLLSFQEFRDLFSDQYIGTGVQL
                     AVGEQTILFTDLVGSTAMYAERGDPAAFVEVKKHFDEVFAIIGKHRGAVVKTIGDAVM
                     GAFNEPLDAVKASRAIHDAFPPSSKPGALRLRVSLNTGTCIAVRLNSAIDYFGHTVNL
                     AAKLQALAEAWQIAMSETTATAHGVADWLASQNAQLEALTIQLKGIDEPTRAKRWTVY
                     T"
     gene            complement(22576..22896)
                     /locus_tag="VMZ53_11880"
     CDS             complement(22576..22896)
                     /locus_tag="VMZ53_11880"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS29209.1"
                     /translation="MDQRAAVRVPVRVRAQFRAHGLVIDGLVEDVSRNGLFMRSPRQL
                     EEGATAEIHLDLPGEETLHLSAQVVRVELAPRAGMALRFVGDVERNRRPLANFIMRQH
                     AVLR"
     gene            23065..23139
                     /locus_tag="VMZ53_11885"
     tRNA            23065..23139
                     /locus_tag="VMZ53_11885"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23097..23099,aa:Lys,seq:ttt)
     gene            23168..23240
                     /locus_tag="VMZ53_11890"
     tRNA            23168..23240
                     /locus_tag="VMZ53_11890"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23202..23204,aa:Glu,seq:ttc)
     gene            complement(23394..23984)
                     /locus_tag="VMZ53_11895"
     CDS             complement(23394..23984)
                     /locus_tag="VMZ53_11895"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUS29210.1"
                     /translation="MTATKKIGIIGNGNVGGALSRGLASARQDVRIVDNEPAQVRATA
                     EWADIVILAVPFGAVSNVATTIAPAVEGKTIIDVTNALTEKMTLALGYTTSGAEELQK
                     LLPSAHVVKAFNTQFAQRMDTGRLGDQRVTTFVSADNAAAKLAVLELANAIGFDAVDA
                     GPLANARLLEPLGYLNIQLGYVLGLGTQIGLKLLRS"
     gene            24103..25719
                     /locus_tag="VMZ53_11900"
     CDS             24103..25719
                     /locus_tag="VMZ53_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HUS29211.1"
                     /translation="MRLDDLDLRELFAFDPQGGVMRFAGERAVIFDAVALGLLRKTLI
                     ETLGTDAARGLMTRFGYVHGRRSAENMKTAVPWDSDDDWRRAGGRLHKLQGLVVPAPV
                     GSARATTPAPFVEDLWQDSYEAEQHLIHLGRSDEPVCWSQCGFASGYLSYANGREIYC
                     IEVRCRGRGDAVCHMVCRPREDWDDKHAPDLAYFEMPRLDAMLTQVTKKLRSAERQLR
                     TRKQELASYRAEPGMTKSAAMNKVLELVERVAQVDSTVLVTGESGVGKERIARMIHER
                     SARAQRALVAINCGALPENLLESELFGHAKGSFTGATQDRAGLFEEASGGTLFLDEIG
                     EVTPALQVKLLRALQEHEIRRVGENKNRTVDVRVIAATNRNLTEEVKAGRFRQDLYYR
                     LRVIEIAVPPLRQRREDILPLARELLAAAATRTKSKATSFTPAVIDQLQRYAWPGNVR
                     ELENALERAAVMARGTRIDVADLPAEIAGAIASSWTPGDERSLKQVEKDYTLAVLEAN
                     GGNRTKTAAQLEIAPATLYRKLAEYGVTGK"
     gene            complement(25744..>25960)
                     /locus_tag="VMZ53_11905"
     CDS             complement(25744..>25960)
                     /locus_tag="VMZ53_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29212.1"
                     /translation="PDHFIPLLYLAGLAKHCKRTPRILVEGYALGSISMTSYTLDCEC
                     PGGDSKQSPIADPLPDPNVVPPDQTNM"
BASE COUNT         4617 a         8753 c         8245 g         4345 t
ORIGIN      
        1 tcacgcgctt ccgcaacgcg ccgatcgatc cattccgcga accaccattc ggcgtcctct
       61 ggcatcgcac cacgctcgcg ttccgctgcc accacgccgc ggccgacggc gagctcttca
      121 tcgagatcgt gcgccatttc ctcgcggctc tctccgccga cgtcccgacg tcgacaaaac
      181 cgatcgacca cccgctcgcg ctccgccatc tccgcaagca agcaaccgtt cgcaacagcc
      241 tccgctacgc gcgctggctc gcgaccgaag cgaaatccaa ccgctcgttg cgcctccacc
      301 tcgccagatc cgcgccgggc ccgatcgcga tctgcgaacg cgtcctgtcc accgccgatc
      361 gcgaccgcct cttcagccac gcgcgcgaag aacgcgtccg ccctgcctgg ctcatcgcgg
      421 ccgcatggat gcgcgcactg cacgcattca gcgtcaccac gaacgacgca acgacgccgc
      481 tcgtctcgct cgaagtcccc gtcacgctgc gccgcggctc cgacatcggc ggcaacgcgc
      541 tcgccgtcac gactctcttc ggcgacgctc gccgtcctct cgccgatctc gcacgcggcc
      601 tctggcgcga ctacgccaaa cacatcaagc aacgcgatca cctcgccgtg cctcttctct
      661 cggcgccgat ccgcttgctg ccatggccgc tgttcaagcg cgtcgccgtg acgactacct
      721 cgacgggatt cgcaacgtcg cacttcacgt ggctcgacca cgcgccggaa ggcctctcca
      781 tcgagcgacg ccacctctac actccggtct gccaacacat gggtgcggcg ctctgcgcgc
      841 tcgcatcgcg cgattcactc caactctcga tcacgcatcg cacgaacggc atttcatctg
      901 cagctgcgag ctctctggcc gatcatctcc tcgaggcact cacttcgcgc gccgtgacta
      961 gcgcagcgtc ttgatgtacg ccacgaggcc ctccttctcg aggtcggtga gcagcgttgc
     1021 gtactccgcg ggcatcgccg gcgggtagcc cggtcgcgac ttcgcgcgcg gctgctcgat
     1081 cgactcgcgc aggtacgcat cgtcgacgac gatcatctcg cccgtgtcga gcgtgatgcg
     1141 agagccaaag tcgtggagaa acgtcgggcc gatgcgcgtc gagccatcga tggtgtggca
     1201 cgtggcgcaa cccatcttct ggaacgcgat ctcgccgact tcctccggcg gcatcgcctt
     1261 cgagatcgta ggcagcggct tgtacttcgg ctgcgggtga ttggacggcc tgccccacga
     1321 gagcgcgagc gtcgtgatga tggccattgc cacgaggact ccgacgacgt ttttgacgaa
     1381 catgcgcgtt cgatgcgcga ggcgccggcg cagggctgtg gcagagcgtc gcgcgtcaga
     1441 gtggccggta gagaaggcca agcagcgcgc cgaagaccag gtgcgacgcg acgacggaga
     1501 gcggggtgcg cgtcccgtaa tgcagcgcga ggaagccggg cggttcgagc aggcgcgtca
     1561 ccgtcgggcc tcgcgtttcg ctcgccattc gcgggtgcat cccgggcaga gcgggcatcg
     1621 cgacgacgag tacgaacatc gaatggactg cgccgatcgc tgcgcccttc caccacgtcg
     1681 gcccgccgga ggcgtggaac acggcgatgt agatgagcgc gaacagccag ccgttgacga
     1741 ggtggacgcc gatgccgagg agctttgcgc gatcgcgatc gggcgtgacc atcgtgccga
     1801 gcaggtacgg gatgttcatg cgcgtgatgc ctaggccttg cgagccggcc atcaaggtgg
     1861 tgagcacgac agtgctcgcg aaaccccaga tgagccagct gccccaattc atgggcgggc
     1921 ctccgcgcga cgaggtagcg cgcgagcgac cggcaagcgg tcggcgatcg agagcgcggc
     1981 gctgatgagg ccgacgtgcg tgtacgcctg ggggaagttg ccgagcgcgc ggccagtgtg
     2041 gacatcgact tcttccgcca tgaggccgag gtcgttggcg taggacgtcg cgtcgccgaa
     2101 tagcgtgagc gcttgctcga gcgagccgcc gccgcggatg aggtgctcga ctgcccagta
     2161 gctgcagagc cagaacgcgc cttcgccgat ctggttgctg gactcgcggc ggtacaagag
     2221 gccgggcatc ggcgagagct ccgcggtgag gcgcgcgtac gtcgagcgca tccgggcgct
     2281 gttcgcgggg tgaaagccgt accaagacat gaggagtacg gcggcgtcga gctgatcgcc
     2341 gtcgagctct gcggtgtagc tctgcaggcc gcggtgccat gcgcgatctt cgatctgacg
     2401 gcgaatcgcg gtgcgctcgt cggcgatacg agcgcgatcg agctggaggt gacccgcatc
     2461 ggcgaggcga tcgaggcgat cgagtgcgac ccatgacagc aaacgcgaat gggtgtggtg
     2521 caccggcgcc gtgcgcgtct cccagatgcc gtagtcgggc tcgcgccaat tctcgatgac
     2581 gtgctcgcca aagccgcgca gcagctcgcg cgtctcttgg tcgatgtggc cggtcgtctc
     2641 gatcacttgc gcgcacgcgt cgatgacctc gccatggaga tcgagctgca gctggcggtc
     2701 agcgccattg ccgacgcgca ccggttgcga gcctccaaag ccttcgagcg gcacgatgtg
     2761 ctcgggcggc ggctggttgc cgtagacatc gtagagcacg cgcaaggcgg gctgcgtgag
     2821 gcgcgttgcg tgcagcagcc agctcgcgaa tgcctgcgca tcctcgatga agccgagctg
     2881 caggaagacg cgcgccgtga acgaggcgtc gcgcagccag cagtagcggt aatcccagtt
     2941 gtgctcgcca cccggcactt cgggcagcga cgtcgtcggc gccgcgatga tcgcgcccga
     3001 tggtgcgaac gacatcagct tgagcacgag cacgctgcgc agcacggcgt cgcgatgcgg
     3061 cccgttgtag cgagtgcggc cgacccactg acgccaccac tcgaccgatc gcgcgatccg
     3121 ctcgctggca gcggcgccga gcggcggtag caccgcgggt gcctcggcat cgaacgacag
     3181 cgaccaatgc agcgccttgc cggcggtgat gcgaacgcgc gcgcgaacgc gaccgtcgtc
     3241 accgagcatg agcgggcgat cgctacgcag cgtgagcagc tgcgagccga tttcccagcg
     3301 aatgccgagt gcgccgagat cgagcgtgcg tgcgcggaag cgtccgaagt ccggctgcac
     3361 gcagaccacc acttcgagct cgacgaatcc actcaagcac tcgacgcgcc gaagcagctc
     3421 gtgctccggc accagctcct tgtgcttgac gtcctcgctc gcgatcgaca tcgaatccgt
     3481 gaggaccacg gagccggtcg ccgtatcgaa gcgagtctcg agcacgttgg tgcgctcgac
     3541 gtaacgccgc gacacgcgtc ccggttcgac cggcgcgatc gaccagaagc ctgcacgcga
     3601 gtcgagtagc gcaccgaaga tcggcgggct gtcgaagcgc ggccagcaca accaatcgat
     3661 gcagccatcg cggccgacga gcgcagccga gcgtccatcg ccgataattc catagtcgtc
     3721 gaccgatacg gcccgtttca tctccacctc cacgccaatg ccgcgatcgc ggaagccgct
     3781 gcgcttgcga cgacgaccgc gcctttgtgc tcgcgccacc actcggtgat cgacgagtcc
     3841 tgcgctctgt cgtcgaagat tccatgcgca ccgaagtcgc gctcgatcgg cagatacaag
     3901 ttgtcgcgat gcttgccgcg atgcccgacc ggcacgtcgt cggtcgtctg accttcccac
     3961 gcatcgcgcg cgagcttctt gtcgagccac cccggcagca cgcgctggcc caggatcgct
     4021 ttcacggtcg gccaaccgat ccagagctcg cgcttgggct tcagtgccgc tcgcaccgcg
     4081 gttcgcgcga tcagctctgg ttgatggatc tgtcccatcg gctgcgggtg acccggcagg
     4141 cgattgcgca cgacctcgaa ctgcggcgtg ttcactgccg gcaagtgcag ctgcgtgacc
     4201 tggatgccgc tcttctcgtg caagagctcg caccgcagtg actcgctgaa cgcacgaatc
     4261 gccgacttcg acgcgcagta ggcgctctgt agcggaatcg accggtacgc gagcgcggag
     4321 ccgatctgca tgatcacgcc gcggttgcgt ttcttcatgt gacgaagcgc tgccagcgtg
     4381 ccgtgcacgt aaccgaggta gttgacctcc atcacgcgcc gatattcagc ggccgtgatc
     4441 tgatcgactg gcgcgaacac cgactccatc gcgttgttca cccagatgtc gatctgcccc
     4501 cagcgaccga cgactgcatc tgcgacgcga tcgacctctg ccgcatcggc cacatcgagc
     4561 gggcacacga gcgaaatccc aagctcgtca gctgtcgcgg cgagcccgac gtgatcacgg
     4621 gcgatcagcg ccaccttcgc gccagccgca gagaacgcgc gcgcgatcgc tcgaccgacg
     4681 cccgccgagg cgccggtgat caccgcgact ttcccagcga gagcaccctc gggaatccac
     4741 ggatcgttgt cgtccatgca acgatcacgt gcgaccgcga tgccaccgcg ctcacgattc
     4801 ttcggagacg tgagctactg tcggcgccgt gacgtgtcgc caccttgctg cgctgactgc
     4861 cggatgcgat acggcattta cagtcgatgc ttcgcgcgtg acgttcgggc ggaattcgct
     4921 gctcgaagtc ggagaccgcg cgagggcgct cggcatgcgc cgcgtcgcgc tcgtcaccga
     4981 tcgccagctg cgtgcactcc cgtggttcga cgacgtctcg agatcgctcg cgaccgcggg
     5041 ctgcgagatc atcatcttcg acgacgtctc gatcgagccg accgacgcat cgttcgaggc
     5101 cgctgcacgg atggccagcg atgcgaagcc cgatgggtac gtctccctcg gcggcggctc
     5161 cgtcatcgat accgcaaagg cggcgaacct ctacgcgacc tatcccgccg cgctccgcgc
     5221 atacgtgaac gcgccgctcg gcgacgcacg cgcgattcct ggtccgctcg caccgcacat
     5281 cgcatgtccg acgacgtcgg ggaccggcag cgaggtcacc ggcatcgcga tcttcgattg
     5341 gcgcgagcac cacgcgaaga ccggcatcgc atcaccgcgg ctgcgcccga ccgaagcgat
     5401 tgtcgatccg cgcacgaccg agacgctacc cgcgaccgtc gtcgcagcga gcggcatgga
     5461 cgtcctgtgc cacgccctcg agtcgttcac cgcgcgcgcc ttctcgcatc gcacctcgcc
     5521 gaagcccgcg accgctcgac cgatgagcca aggcagtaac ccctggagcg acctcggctg
     5581 ccgcgaagcg atgcgcctcg ccgcgacgta cctcgagcgt gccgtgctcg acgcagccga
     5641 cagcgaagcg cgcgagcaga tcatgtgggc cgccacgctc gcaggtatcg cattcggcaa
     5701 cgcaggcgtg cacgtgccgc acgcgatggc ctatgcggtc gctggtcgcg ttcgagactt
     5761 cgcaccagcc ggctacccgg cgacgccgat cgtgccgcac ggcatggcgg tctccgtcgg
     5821 tgcgccgtcg gtgttccgcg cgttcggcac attctgctcg gaccgccacc tcgaagctgc
     5881 ggcgctcctc ggcgctgata cgcgcggcgc ctcccccagt gacgccggcg cgctcctcgc
     5941 cgatcgagtc atcgcaatct cgcaatcgat cggcatcccg aacggcatgt ccggcgttgg
     6001 ctacacgcgt tccgacatcg ccgacctcgt cgccggcacg ctgccgcagc agcgcctgct
     6061 ctcgaatgcg ccctgcgacg tcgacgccga gtgccttcac gcgctattcg aaggtgccct
     6121 cgcctactgg tgacgcatgc ccgccaaaga acgcccgacg atcgacacct acccgtacgc
     6181 caccgcgatc acgacgcgct ggatggacaa cgacatctac ggccacatca acaacgtcac
     6241 gttctatagc ttcttcgaca cggcggcgaa caagttcctc atcgatcgcg gtggcctcga
     6301 cattgccaac gatccggtca tcgcgctcgt cgtcgaatcg aactgcagct accacgcgcc
     6361 gctcgcgtat ccggccaaca tccgcgcggg cgtgcgcgtc gctcacctcg gcaatcgcgc
     6421 cgtcacctac gacatcgcga tcttctccga ccgcgatccg gccgccgccg cgaacggcaa
     6481 gttcgtccac gtgttcgtcg accgcgcgac gcgccaaccg acgatgattc ccgccgccat
     6541 ccgcaaggcg ctccagactc tgcaatgacg ctcgaagttc gcccggttga agcacgcgac
     6601 gttcctgagg tgctcgagct cgtcgccgaa acgctcgcgg agttcggcct cgagttcggc
     6661 aaaggctcgc cgaccgacga ccaactacgt cagctccccg cgtcctactc cgatcacggc
     6721 ggcgcgttct gggtcgcgct ccgcgacggc gagctgctcg ggactgcagg cgtatttccc
     6781 gtcgaagacc gcatgtacga gctccgcaag atgtacctgc gccccgcatc gcgcggcctc
     6841 ggcgtcggca agcgcctgct ccatgccgcc gtcgattggg tccgcgcccg cggcggtacc
     6901 cacatcgtcc tcgatacgat cgaagagatg acgcgcgcga tcgcgttcta cgaagcgaac
     6961 gggttcgtgc gggatgatgc gcagaagcgc gcggcgcgct gtactcgcgg gtacgcgcgc
     7021 aagctctaga atctttgcga tcgcccgacg tgataaagtc gtaggagtga tcgagggaag
     7081 tacgatcggg caataccgcg tcatccggaa aatcggtgaa ggcggaatgg gtgcggtata
     7141 cgaggcgcag cacacactga tcggacggcg agccgcgatc aaagtactgc tcccagagct
     7201 gtcatcgcat cgcgagatcg tcgaccgctt cttcaacgag gcgcgcgcga cgacatcgat
     7261 ttcggatccc ggcatcgtcc aggtcttcga cttcggcgtc cacacggaca acagcgcgtt
     7321 catcgtgatg gagtttctcg agggcgagcc cgtcgattcg cgaatgaagc gatttggccg
     7381 gctcactccg ttcgacgcac tccgcatcac gaggcaatgc gccggctcgc tcgccgctgc
     7441 acaccagcgc ggcatcgttc accgcgacct caagcccgag aacatcttcc tcgtaaaaga
     7501 tcaggaagcg cagggtggcg agcgcgcgaa gatcctcgac ttcggaattg cgaagctctc
     7561 gaacaacgaa tcgagcacca agactcgaac gggcatgatg atgggcacgc ccgtctatat
     7621 gtctccagag cagtgccgcg gggcgggctc ggtcgatcac cgctccgaca tctactcgat
     7681 gggctgcgtg ctctatcaca tgctgacggg ccgcccgccg ttcgatgccg aaggcatggg
     7741 cgagctgatc gcgatgcacc ttcgcgagca acccaagcca ccgagcgagc tcgtgcaagg
     7801 gatcaacatc gtcgacgagg tcgtgctcac gtgtctcgcg aaggatcccg ctcagcgcct
     7861 ccaaacgatg accgacctgc agcgcgcgtg cgacgcagtg atggcgcgga tcaccaacag
     7921 cggcgcatcc gcacaaacgg tcgcgctcgc aacgcccgtg cctcctggct ttcgatccga
     7981 gttccctcaa gccgccgcgg cgccgctgca acccgcgacg caggtcctcg gcaagccgac
     8041 gacactcagc gcagctacgg gcgaggccgg aaccactccg ccaacgccgc gtcgtcgcgt
     8101 cggactcttt gccggcatcg gcgttctcgt cgccggcggt atcgtcgcgg cagtcgtgat
     8161 ggctggcggc tccaaaccaa agccagagag cggtactgcg gcgacaccgg gctcgaatgc
     8221 cgcgcctgtc accgccgtgc cggcagatgc gccagctgtc gtgactgcgg tgccgccgga
     8281 tgcagcgccg gtcgcgccgc ctgacgccgc cgagatcgca acgtcgccgc cggacgccgg
     8341 cacgaccgcg gccgccgtcg tcacgactcc agtcaaagat ccgaagcgac cgaagaagcc
     8401 gactacgacc aagcccaaca cgggctccgc ctctggaggc aacctctatg acaatcgcta
     8461 agctcgcact ggccgcgctg gtgctgttgg tcgccgttcg tcccgcgctc gcgcagagcg
     8521 tcgaagccga ggcgttgttt cgcgaaggca aggagctgct caaacaaggc aagcttggtg
     8581 aagcgtgcgc gaagttcgac gcgagcgatc gtatcgaacc gagcgtaggc accgagctca
     8641 acgctgccga ctgtcacgag aagaacaaca agctagcgac cgcgtgggct gggtttctca
     8701 aggccgccgg aaatgctgcg aaggcaggca atgacaaaca gcgagaggct gaagcgaagc
     8761 ggcgagcgag cttgctcgag ccgaagctct cgtacttgac gatctcggtc tcggagtcga
     8821 gtcgcgtcac cggactctcg atcaagcgca acggcgtcga tgtcgaccaa gcattgtgga
     8881 acacgggtat ccccgtcgac gccggcgact atgaaatcgc tggccaagca cctgggcacg
     8941 aagcctggag cacgaaggtg acggtcgcgg tctccgagcg aaagagcgtg gaggtgccgc
     9001 gcttcaagga gatcacggcg ctcgtcacgc cgccccccga tcaaaacccg aatccgctcg
     9061 agaaggaacc tgtcgagcaa ccgcacgaga cacccagcac cttcacgccg atgcgaaagg
     9121 ccgcaatcgg agcggcagcc gtcggtgtca tcggcatcgc cgggacggtg gtgttcggct
     9181 tgaaggcgaa cagcaagcag agcgaggcgg aggacctctg ccctgctgca ccggcatgcg
     9241 ccgacccaat ggcgatccag ctcaacgacg acgcacggag tgcagcgaag ctgtcgaacg
     9301 tgatgctcgg cgtcggtgtt gtcggcgttg gtgccgcggt cgcgctgtgg tttatcggcg
     9361 cgccgaaaga aggcacaccg aacgacgtcg cgatcgttcc ggtcacgaac ggacgggacg
     9421 tcggactttc attctcgggg agcttctaac gaatgcgcgt atcgctttac ctcgcgctcg
     9481 cgtttgttgc gggctgtagc gtgccggact tctttctcgt cgatgcaggc cacggcggcg
     9541 atgacggtgg tgccgacgcg aaaccggacg ccgcgctcga cgcaccacca ggcgcggcaa
     9601 cgatcagctt tgtgagctcc agcaacaccg acggctacta caaggccgcc gacacgatct
     9661 cgatccaagt aacgttcagc gaagccgtca ccgtcgatac gaccggcgga acgccgacgt
     9721 tgacgctcaa cagcggagcg agtgcaagct acgcgagcgg cagcggcacg acgatcctga
     9781 cgttcagcta tacggtcgcc gctgggaacg ccgctgcgga tctcgaatac acgacggcaa
     9841 cggctctctc cgcgaacggt ggcacgatca agaacggaag cgtcaacgcg ttcctggtgc
     9901 tgcccgtccc cggtgcggcc ggctccctcg gcgcgaacaa ggcgatcgtg atcgatgcgg
     9961 tcgcccccac gatctccgca gtcacgggcc ccaacgcgta cgcaccgtcg gcgtcagcaa
    10021 cgctctcgta cgcggtcacc gatgccaatc ccgggaccac gacgtgtacg caagtgacgg
    10081 gcaccggcac gatggcgagt tgcaccgcaa ccggcgtgac gttctcgggc ctcaatcaag
    10141 gcgcgcacac gatttcgatc acgcataccg atgcagcggg caacatctcc gcggcccaga
    10201 cgtactcgtg gacggtcgac acgactgccc ccgtcgtttc gacgatcagc gggccggctc
    10261 tgttcgcgcc atcgacgagc gcaagcttga cgtactcggt caccgaaacg aaccagggca
    10321 ccacgacctg cacgttcacc acaggtagcg gcacgatcac gagctgcact gccagcggcg
    10381 cgtccgtgac aggcatgaac caaggtgctc acacgatctc tgtcacgcat acggacctcg
    10441 ccaccaatgc gtcgactgca cagacgtact cgtggacggt cgataccgtc gatccagtca
    10501 tctcgatcac gggccccacc aacaattgga cggcaggcaa taacgtctcg ctcacggttt
    10561 cgatcaccga cgcaaacccg agcacgacgt cctgtgctca ccccgtcggc accggtactc
    10621 aaacgaactg cagccccacg gcccttactt atacgggcct cagcacgggc gcgcacgagg
    10681 tccacgtcat ccacaccgat cgcgccggca acacgacgga ccagacgtac acgttcagcg
    10741 tttgtccgcc ggtcagtttc gcgacgccaa gcattcccaa cttccaggtg cccaccggct
    10801 gcggaaccgt gatggacgtc caggcgtggg gagctggcgg cgccggtggc gcgtccaacg
    10861 gtggtgccgg cggtggcggc ggttacgcgc gcggtacgct gacagtcgcc gcgggacaga
    10921 gcttcatcat cagagtcggc ggcggtggga ctcggtcgac caccggtgcg ggcggtcgcg
    10981 gtttcaacga cggcggcagc gctgggctca acggcggcgg cggcggtgga tggagcggcg
    11041 tcaatttcgg aaatggaagc gacatgcttc gcgcgcccgg tggcggcggt ggtggcggct
    11101 gtaacggtgc cgccgcgggc ggaggaggcg gtgggctcaa tggatcacct ggcggcggtt
    11161 ccttgcccgg cggcggcgca agtcagggtg cggccggtgt cggcggaggc actggctcgt
    11221 cgggtggcgt tgggcagggt ggcagtggcg gagactcggc gagcggctgc agtggcggcg
    11281 gcggtggcgg cggcaggttt ggcggcggtg gcggtaacgg tcctaatacc ggcccgagca
    11341 gcggcgctgg cggaggcggc tcggtcttcc tcgactcgag tctcgcatcg acgatgacgc
    11401 aagccggcac gggcgtgaac gccggcggtg tcgccgagcc caactacatc aacggtcgtg
    11461 gcttcggtgg cagcagcaac ggcggcacgg gtggcgacgg acttgtcgtc ctgcacgtcc
    11521 acccgtagcg gccggcgtta accgcgcaaa acgctagtcc tcgacgcagt agaagcgaat
    11581 gttcgtctgg tcgcaggtcg acgtcgtttg caaattatac gcgcgcgagt cgagcaggtc
    11641 gtaacggccg atgtaccagt tgtagaagct cgcgttcgac gaccagttcg agcagaccgc
    11701 tgacgaagat ccgtagtact gccccgtcgt tcctgaggcg ccgtagatgt tcgtcgtcac
    11761 gctaccggta tcgccattac ccgtcaggta cgtgccgagc tcggtctgat tccacgcggc
    11821 agacagcttg ctgtcgagca tgtcgttacg cgacgtgccg agcggaacgc cgtcgggacg
    11881 cacatacgtg aacgtcgtcg acgtgaactt gttcgagatg tacgacggat acgcgagcca
    11941 cgccttgaac gctgccgcgt tcgcaaaccc agcgtaacgc gcgcgggcct gacaaatcgc
    12001 atccggtgca ttcgaacccg tttggccata tgaatcgggc cagctgctca gcaagccgtt
    12061 gccttggacc gacgtgatga aggctttctt cacgagcgtc ggatggcgag gcggcagcgc
    12121 ggccggtgtt gttgcgctca cctcgacgca gcgcaagtgg aacccggtag tgttgttgca
    12181 gaacgggttc gtcgcgctgg tctgcgtcca cgagctgccg ccgccgtgca cttcgcccgc
    12241 gacgccagca aatgacgagc tgctcgagag ccagtcgttg cacgttgcgc cggcgacgta
    12301 ttgcccggaa ttgtccgtgc ccgtccacac gcggtccgtt gtcgacgtga tctcgttgcc
    12361 attctcgtcg cggtttgcgg ggaagtaggt gattgaattc ggagtgagaa gcttgtcgat
    12421 cgcgggagac cattgcttcc aatcaccgac gcgaacccac gggcccgcgg cgatcggcag
    12481 ctggccaagg ccgcagttgc ccgccttctt gccattgagg tgccccgtcg cgccgtccgt
    12541 ttgtgcacgg cagtacgcgt cgtgcgtgga gtcggagatc caggcgacgt acgtgccggg
    12601 cagacccgac ggatacgccg tggacgtgtg ggctgcggcg cgcgcctgac agatcgcatc
    12661 cgccgccgcg agaccgttgt ttgttccagc gccgagccag ctcgacagca aaccggagcc
    12721 cgtcacagat gtgacgaacg tgaagcgtcc acgcttgcag tcaccgacgg cgccgcttcc
    12781 gaagtagccc gctttgcata cgcacgacgt cccgagaaac tgcgcgttcg aatcgcagtc
    12841 ggtcgataca gtcgttgtca agctgcacgt gtcgccatcg ccgttgtagc cattcccgca
    12901 cgcgcacgtg tagccgttcg tcgtgtctgt acacgccgca tgcgcatcac actgggcgta
    12961 cgctgtgccg cactcgttcg ctgcgaccgt cacgtcggcg atcgcgccgc ccgaatacac
    13021 cggaccgccg acgccgttcg tgcagcgcag ccggaacgtg cgcgcttgcg tgagcgtgag
    13081 cgtcgtcggg gtgcccgacg tgatcgtcgt ttgtccgtta tcgatcgagc acgtcggcac
    13141 gggcgatggc gtgttcgaat acgtccacgt gaacgtcagc ggcgtcgcgg ttcctggcgt
    13201 caccgacgaa ggtgtcaccg tgaacgtcgc gatctgcggc gcggaaggcg tcacattcac
    13261 gatcacctgg cgcatcccct ggcccgctcg gttcgagcac gtcatcgtga acagcgtcag
    13321 cgtggagatc gtgatcatga cgggcacgcc cctcatgacc gtacccacgc cgttgtcgat
    13381 cgtgcacgtc ggctcggggt acgtcggctc gaccagatat gcccagttga acgtgaccgc
    13441 cgttgggaca ttcggtgaca gccccgacgc cggcgatgcg gccaagccgg taatgaccgg
    13501 ggcgaactcc ggtgcatccg ggatgacgac aactgcggcg tcgctcgggc cattgcccga
    13561 atcgtcacca caggcggcaa gcgctagcgc gagtaaccca atcttcgtga actgcatcgt
    13621 gccccctctc gtgaaccgct tcatggactc ggaatgctgc cgacggccgt gcagtagtcc
    13681 gcggtgatcg tcgtcgacgt gcagacaacg tcacccacgc attgggatgg aacgcacgac
    13741 tgcgtgaaga agcatgttcc ggccgacgcg gcgatacacg tcgcgtcgct cgtgcacgtc
    13801 tgcccgttgc cggtgcactg gccaccgctc gggttgcaat ccgggtagct gttcacgcag
    13861 ctggtggtcg gcgagagcga acagcgtccg ccgcagttta gatccgagta gcaattctgc
    13921 gacgtgccgg agcacttgcc tggattgcag gttcccgaga tgcacgccgc gtttgcgagc
    13981 gcctgggagg tgaattgcga gcttgctgcc tgatggcagg ccgcgccgcc ggcgcccttc
    14041 ggtgcgcaaa cgtcgtcggc aggatcgatc gtgttcttgt tgtcgcagaa caaatcggtg
    14101 ccgcactgcg tgctgctggt gcatgccgcg ccggctgcaa ccttgggcgc gcatgtgctc
    14161 gttgttggct ggcagtaata cgcgctcgcg cacggattgc tggtgctgca ggggaagcca
    14221 gccgtcggct tcatcttgca cttcaggtct ggaccgcaga atgcgtcggg cgcacctgcg
    14281 cagtcgaacg ggaagaggca ggcagctcca acggcgacag tgccgaccca cggagcgtcc
    14341 ttgcaggctt ccttccacgg gaccgtcgcc tcggcgaccg ccgagcaggt gtccgtcggc
    14401 gcgaggatcg cctcgaggca accgttgagc ttcgcgggat cgaacgtgat gcgaccatcc
    14461 tggagcgagt cacgaacgtc ggccccaccg cgctgacaca agcgcgagta gtcctggcgg
    14521 cactgcgcct cggtgcgcgg ctcgctcacg ctgagacgat cttcgatctc gctctcggtg
    14581 cagcattgat agaggttgtg acagacgacg tccgcgatct gctcgcagaa gttgtcctgg
    14641 ttcgtcgaga tatcgacgct gctgtcgccg ccgcacgcag ccgcgagcaa gccaaaagcc
    14701 gaaatcaaag ctagacgcat gacatctcct cgttgagtac tcaccacgtt ctctcccaga
    14761 caaaggtgcc gccgccgctg cgcggcttga atgaaatgga tgtgtcgtgc tccttcgcat
    14821 tcttgatgcg aacgaccgca acgacgccga gcgcgacacc gacgccaaca cccacggcgc
    14881 cgaggacata ctcggtgctc gcgcgcgtat cgatcgcgtc gtggtcggag aacttcgccg
    14941 cgcgatcggc gtccttcgaa tcgttgtagc ccgagagcag cagcacgacg ccagcaccga
    15001 tgccgacgcc ggcgcccatc aagaggccca tgttcgcgcc gctgatgctg ccgccgctgc
    15061 tcgatgtgac gacggccgga gcgggaggct cgggaggctc cggtgcgggc gcgggctggc
    15121 tcggaggcgc cgcgaccgag gtcgctggac acagcgccat gttcgtctcg acgtccgttt
    15181 tcctctgcgc gtcaggggca acatcgacga gcgcgcggta cagcgggacc gctttgtcgc
    15241 aatgaccaga ctgccggtac gactgcgcga gcatgaaccg atactcgaag ttgttcggct
    15301 cgagctcgac cgccttctcg agaggcgcca ccgcgccggc gtaatcgccc gcccgatata
    15361 aatccgtgcc ttgctttgca aaatcggccg acggcaaggg tccaggctgc gccagcgccg
    15421 tcgccgacgc gaacacaacc cccactgccg aaacgagagt gaacctcgat cccacggatc
    15481 ctccttgagc gggcccccgc acgctctacc agaacgataa cccgaaaaac tcgcaagcgc
    15541 gcaatttgcg ccgtgactct cgtctcataa aacggcactc gcgagaaatc gttcgccccg
    15601 ctcgtgcgtt gaacgccaga tgcgccgctt gttgccagcc ctcgcgatct cgatcccgct
    15661 cgtgagtctc gtcttcgccc ggcacttgag cgcaactaca tcgtggctaa cgccgttcga
    15721 gatcgcgatc tcgatcgcac tcggcatcgc gtcgctcacg acgatcgttg cgctgtttgc
    15781 gggctggaag cgcgtatcga tcgcggttcc cgtcgcgctc ctttcgatca acgtcgtctt
    15841 catgctcgcg cgcgccgctg acgtaaagct cggcacgttg ccaccgccgg atgtgaagtt
    15901 cgcgatcgtg ctctccgccg gcatgctcct ctcgatggtc ggcctcgtga tgcgccgtcc
    15961 atgggggcgt tggctcggga tggcgtttgg tgctgctgcg atcggttgcg gtgcgctgaa
    16021 cctcgtctgg tattggcccg cgagcggaac gccgaacgtc tcctacccgg agtggtacgc
    16081 ggacgtctgc cgcacgacgt gcttccacat ggtgacgtgc ctcggtggcg cggtgatcct
    16141 catcaacacg atcgcggcat cggacatctt ccacacgagc gccacgtgga gccgccgcga
    16201 aggcgtgatt cgctggctgc gcgcctcgat ggtcgcgacg ttcgtcgccg tgccgatgct
    16261 gctcgtctat gcgtggatgc agccgctcgc agcatcgacg aagccgaccg cgctcgctct
    16321 cgccgccgtc ctcgcagtcg gcgcgttcct cggcgttcgc ggcaagctcg ttggcgcact
    16381 gctcctcgtt ctgggcggcg ccggtctcgc cgtgcagacc gcgatgacga tgtcgctcac
    16441 gcacgggacg acccgcagca tcagctacta ctacgccgcg ttctggacgc cctgcgcgtt
    16501 gatcctgctg atcaccggct gcgtgctcgc ccgaccgacg ctgcgcctgc tccgcggctg
    16561 agtccccaag taggcgggcg ccgggggaaa gcctcagccc ccacgggaca agtccctgat
    16621 cgcacacggg agtctcggtg gcatgtcggc tgctctaagg gatggctgcg caacccggag
    16681 catccaccat gtcacccgac gacgatgacc tcccctctat cgatcccgcc gccctcgaca
    16741 ccgtcaccgg cggtacggcc tccaacgacg acgtcacggc agcgatcacc tcgctgtcga
    16801 acgacctgaa ggacttcgtc tcgagccaga agaatcaagg ctcgaacacg ttccagcaga
    16861 tgctgccatt tctgctcatg atgagtggcg gaaactggag cttcagctcg agcggcggct
    16921 tcaactgcag cggcggcggc ggcggctgcg gacgctgcgg tcgtcgcggc tgcgccggca
    16981 actgctgatc acgagcgaca gccgatcgag tccatcagca gcagctgctc gttctgcatc
    17041 gtccgatcac agctgttgcc ggcttccgga agcgttgccg taccccacgt gcacgcctgc
    17101 gtatgcgcgc acgtcgagct gacgcacatg cagtcgcgcg cgctgcgcgt cggcacgaga
    17161 ccgacgaggt gaccgaacgc tccggcgatc gtgtccgcgt atgcgcgatc cggcgtgtcg
    17221 agcgtcgcgt cctgctcgaa cacgaacgcg atgccgttct tgttcatcgc atcgcacacg
    17281 ggcttcaccg cgaggagctt gtcggcgacg ccggtgatcg cgtccgatgt gccgccgacg
    17341 gagaccatcc agtagttgcc gctggcgggt cgcgtcgtga cgacatcgac ggagaacggt
    17401 gcaagcgcgt cttgcgccat gccgacgatc gtcgagatgt ggctcgcgcg cgcggcgtcg
    17461 cccgagagaa agctcggcac gacggtcgat gcttgcgtca cgagcgccga gcaattcgtg
    17521 cgcgagtcat cgcagtcgcc gagcttgact gtcacgcctt cgaacgagac gaacagcttg
    17581 aacgtgcccg tcgcgagtgg ttgcgcagcg ggaggacacg acggtggagc atcgggtcgc
    17641 ggccgcgcgt cgttcgacac gggcttctct tctccgccgc acccaacgat gatgaaacct
    17701 gcgagcactg cgaacgtccg gacagaaagt ttcatgcgct gacggtagag aactgcgcaa
    17761 gcgccgccca ggtacacgcg gagcgacgtc gcgcgacggt cgcgccatgt cccgcgacac
    17821 gtcgcagctc agtaacccgg cagaatgaag atcgcgtagc cgacaaacac ggtgaagtaa
    17881 ttcagctcgt cgagatcttc gcgcacgccg aatacgacgc ccgacgaatc cgcgccgtgc
    17941 tctggatgtt tcttgtcgaa gcgctcgcga agaggaacga agccaccgag ctcgaacgag
    18001 ggaactgcgc tcgcttccgg tgcgcccgaa acgatcgcgc gccagatgcc gatgcgtgca
    18061 tcgagaaaaa acgacggcac gatcttgatg ccaccgagcg cactcgcgcc cgcggtccaa
    18121 cgcgatccac cgctcgcaac atcgacgcgc gtgtcgagat cgatcgcgag catgtacggg
    18181 ctcgtgctca cgcctccacc gccgatgcca acgtgtccgg tgaccgaacc acctttgggt
    18241 gacaacgtgg gttgctgcag tacgccgccg tcgatgctcg cgacgcatcc accgacgaca
    18301 acgagcaacc acgcgaagct ccgcatagat cggttctatc cgacatgtga tcgattcgca
    18361 tctcacgcgg gagcgcgatc ttctctgacg attgttggtg acaaaccgca ccgcggttct
    18421 ctagattaaa aattgtcagc gactcactcg tcggaaaccg aaccgaaagg aggagcgtga
    18481 agagcacgac tcgtgtgagg gccggcccta gctagccaca accgagcaag aggcaaggca
    18541 ggaacgatcc tgcgccacga aacggcaatc ctcgcgcgag cgggggaacg caaagccacg
    18601 ggactcattg agtcagccga gccgccgaag gaggcacgtt tgcacgcgag agacgatccg
    18661 tagctctcat atccgaggct cttcgcccgt tacgttgtca cgatcgcgag cccgtcttgt
    18721 cccggcgtag ccgcggacct gttcactccg atctgcacgt ccggcgaacc acctcgcacc
    18781 aaagcacgct cagctctgtc gggccccctc gccgcctcat cgcggttgca gggggttcgg
    18841 cgctttcggc gacgcgatac gatgaccgcg atggcgacgg aagacgacga gcgcggaacg
    18901 gtcgtggacc ggcccaccgc gcgcgagtcg aagccgcaca tcgagagtgc gcgcgacgcg
    18961 gcgacagcga tcctgtcatc ggctcccggg ggcaccggcg caccagggtc gtcgctggcc
    19021 caagcggatg tgccttcgcg ttcgtcgagc tcatcgctcg gcggtgcgga ctcgctcggc
    19081 gccagcatgt cgacgatcgc gaccgcacgc ggagcgctcg atcactccga ggtggtgcgc
    19141 acgcgcaact tctgcctcgt cggcatgacc atcgccgcgg caggcctcgc ggcaatcccg
    19201 ctccttcccg gcggctactt cacgacgcgc ctgttcatgg tgtgcatcgg cgtctgcatc
    19261 gcctgtctcg tctacatcct ctaccgcacg cgcacgcccg agacgtttca cgccggctcc
    19321 gcgatcagca tcgcgtggtt catccccgtc gtcggtgtga cgagcgcgat tccgtttttc
    19381 ggcgcgatgt cgcctgcgcc gatcctgctc gtgctcggca tcttcttcaa cgggctcgga
    19441 accacgcgcg gcgtcggcgt cgcgacctat gtcatatgcg cgctcgtgca aggcgtgacc
    19501 ggctttctcg tcgcattcgg cgtgatggca gatccgggct tcatccatcc ggtgcacctc
    19561 gcgcgcgaat acgaagtcac ctgccagatc ctcgtgcagc tcattctcgc cggcacgttc
    19621 gtcgtcgcgc gtgcgtcgcg tcgctcgcac ctcgccgcac tctcggatct cgaagtcgcc
    19681 gtccgtgcga tcgcgcagcg tcaggcgctc ctcgaagaag cacgtgccga gctccgccgt
    19741 gcgatgggaa gcacgcgcgg gcgcttcacc gatcaagtga tcggcgtgta tcagctcggc
    19801 gacctcatcg gacacggcgc gatgggcgag gtctatcaag cagtcgacac gcgcgacaac
    19861 caagccgtcg cgatcaagat gctgtcacgc gcgtcgctcg gcaacacgca gcacgtcgag
    19921 cgcttcatgc gcgagctgcg cacgacgacc gcgatcacgt cgccgcacgt cgttcacgtc
    19981 ctcgccgtcg gcgagcatcc acttcctcat ctcgtgatgg agcgactacg cggccgcgat
    20041 ctgtcggcga tcctcaagtc gcgcgggacg ctgaacacaa aggcgacgat cgagctcgtg
    20101 cgccaggtcg ccgaaggtct cgccgcagct gcgagcgcgg gcatcgtgca tcgcgacatc
    20161 aagccgcaaa atctcttcct cgccgacggc acgtggaaga ttctcgactt cggtgtcgcg
    20221 cgcgtggccg cgagcacgga cacgctcacg caaggccatg tcatcggcac gcccgcgtac
    20281 atggctcccg agcaggcgcg tggcgcgcag gtcgatcacc gcgctgatat ctacgcgctc
    20341 gctgcggtga tctatcgcgc gctgaccggc cactcgctgt tcaaaggcgg cgaagtggcc
    20401 gacatgctct acaaggtcgt gcacacggcg ccgcgcaagc cgagcgggct cgccagcgtg
    20461 ccgacggatc tcgacttcgc gctcgcgatc ggtctcgcga agaagcctga cgatcggttc
    20521 gcgaccgcgg gcgagctcgc cgatgccgtc gaggcggcgc tgaccggcaa gctatctgca
    20581 gcgatgcgcg agcgagccga gaagattacg aatgcatggg aatccgaaga ggcgtcgccc
    20641 cgtcggtaag tagccggcca ctccgattcg tgggatcttt ggctcgttcg catggcggca
    20701 ttggccgaga ccgcagacac gctgcgcagc actgggatcg cagagctctt agcggcgcat
    20761 ccgtggccgc gcgaattcgc cgacgcgcgc aggctcgagt ggctctggac cgtcgatgtc
    20821 tcggtcacgc ccgaggtgct ctggccgctg ctcgccgatc tgtcgcgcct caatcgcgcg
    20881 ctcaagctgc cgcagatgac gttcgtcgag aaggaaggcg tgcgctgggg aaccgcgcgc
    20941 tacaccggcg tcaaacacga gtggcaagag gtcccgtgga actgggtcgc cggtcgctgg
    21001 tacgagctcg ttcgcatcta ccgcaaaggc tcgatgcgcg cgctgcacgg catctacgac
    21061 ctgcagccga cgccgaccgg cacgcgcgtg tcgatctact acggcgtcgt cccgagctcg
    21121 cgcatcttcg acctcgggct cgagttcaac ttccgcgcga tgggacgcgc gtacaaacgc
    21181 ctgctgccgc ggatcgccga agaagcgaag cgcgagccgc acgccccgcc ctgcctccgc
    21241 gcgccgtcac cgaagctgca gcccgacgcg caaacacgcc tcgaccagct cgcgaagagc
    21301 ctcgaagacc aaggcctcga tcagaagctc gtgcgtcagc tcgtcgactg gctcggctcg
    21361 gcgaccgatg aagagctcga gcgcattcgc gtccgcgagc gcgcgcgtgt gtggtcgatc
    21421 gacgaagacg agctacttcg cctcttcctc cacgcgacgc gaacgggcct tctcgatctg
    21481 tcgtgggacg ttgtctgccc gcactgccgc ggtgtacgcg acgcgaccgg acagctcgac
    21541 tcactaccga cgagcggcca gtgcgacgcg tgcggcatcg cgtttggcac cgacagtccc
    21601 gacgccgtcg agatcacatt ccacgcgcac tcgtcgatcc ggcccgtcga acgcggcgtg
    21661 tactgcagcg cagagcccgt tcacaagcca cacatccaca tccagcacac ggttcagccg
    21721 ggcgagcgcg cgacggtcac cgtcccggtc ggcgccggca agtaccgcat gcgcgtgcgc
    21781 ggcgaaatgc gcggcgcgtg gctcgagatc gccgacggcg ctcccaccac gctcgcctgg
    21841 tcctccgccg acctccccga aaccgcgaag ctctcgtcga agggaacgct cgagctcaca
    21901 aacaccggcg acaaacccgc gacgttcgtc gtcgagaccg ccgtctggct cgagctcgcg
    21961 cttcgccccg gtcgcctcct cagcttccaa gaattccgcg acctcttcag cgatcaatac
    22021 atcggcacgg gcgtgcagct cgcagtcggc gagcaaacga tcctcttcac cgatctcgtc
    22081 ggctccaccg ccatgtacgc ggagcgcggc gatccagccg ccttcgtcga ggtgaaaaag
    22141 catttcgacg aagtgttcgc gatcatcggc aagcaccgag gcgcggtcgt caagacgatt
    22201 ggcgatgcgg tgatgggcgc attcaacgag ccgctcgacg cggtcaaagc ctcacgcgcg
    22261 atccacgacg cgttcccacc gagcagcaag ccgggcgcgc tccgcctgcg cgtgtcgctg
    22321 aacacgggca catgcatcgc agtccgcctc aactccgcga tcgattactt cggccacacc
    22381 gtcaacctcg cggcgaagct gcaagcactc gcagaagcct ggcagatcgc gatgagcgaa
    22441 acgaccgcga ccgcacacgg cgtcgccgac tggctcgcgt cgcagaacgc tcagctcgaa
    22501 gcgctgacaa tacagctcaa aggcatcgac gagcccacgc gcgcgaagcg ctggaccgtc
    22561 tacacctagc tcagctcacc gcaagacagc gtgctgacgc atgatgaagt tcgcgagcgg
    22621 gcgccgattg cgctcgacat cgccgacgaa cctcagcgcc attccagcgc gcggagcgag
    22681 ctcgacgcga accacctgcg cagacaaatg cagcgtctct tcgcccggca aatcgagatg
    22741 gatctccgca gtagcgccct cttcgagctg ccgcggagac cgcatgaaca gcccattccg
    22801 cgacacatct tcgacgaggc catcgatgac caggccgtgc gcacgaaact gggcgcgcac
    22861 tcggacaggg actcgaacag ctgccctctg gtccatgtat tgaaagctac gctatccccg
    22921 caccaccacg taagcaaccc tttcgatccc ctcacgcttg ccgcatcgcg gcgaaaccgt
    22981 tcgagaagat cgggagtgcg gtcggaccgg ctcgcaggac attgctttct acgctcgggt
    23041 acggcagatt gcgccaccca aactggggct atagctcagt ggtagagcac cggcctttta
    23101 agccgagggt cgaaggttca agcccttctg gcctcaccag tagatgaagt aaacaaacag
    23161 cgactcagac cccgtcgtcc agaggcctag gacacccgcc tttcacgcag gtaacacggg
    23221 ttcgaatccc gtcggggtcg ctcaccatca ctcgctcgcg cgactcgctt cgctcgccgg
    23281 cgctcgctca ctccggttcg aatcccggga atcccggaga acggttccgt cggtcgccat
    23341 tcgaccaaca tggtccaaca agctcgagaa gtgctttgta ttgtctgctt tcgctagctc
    23401 ctgagcagct tgagtccaat ctgcgttccg aggccgagca cgtagccgag ctggatgttg
    23461 aggtagccga gcggttcgag gagacgcgca ttcgcaagtg gacccgcatc aacggcatcg
    23521 aagccgatcg cgtttgcgag ctcgagtacc gcgagcttgg ccgccgcgtt gtcagccgag
    23581 acgaacgtgg tcacccgctg gtcaccgagc cgccccgtat ccatgcgctg cgcgaactgc
    23641 gtgttgaacg ccttcacgac gtgtgcgctc ggcagcagct tctggagctc ctccgcacca
    23701 ctcgtcgtgt agccgagcgc gagcgtcatc ttttcggtga gcgcgttggt gacgtcgatg
    23761 atcgtcttgc cttcgaccgc cggcgcgatc gtcgtcgcga cgttcgacac tgcgccgaac
    23821 ggtacggcca ggataacgat gtccgcccac tccgccgttg cacgaacttg tgcaggctcg
    23881 ttgtcgacaa tgcgaacgtc ttggcgcgca gaggccagcc cccgagagag agcgccgccg
    23941 acgtttccgt tgccgatgat tccgattttc ttcgtggccg tcatgttcat tctccttgtt
    24001 gaccagagat agagcatggg gcgtgccgac cgaggccgtc gtaaaatcag gcgtttgcgt
    24061 cgcttcggct ctcgaaatga gataagacac tctcatcctg atatgcgcct agatgacctc
    24121 gacctgcgtg aattgttcgc tttcgatcca cagggtggcg tgatgcgctt cgcgggcgag
    24181 cgcgcggtca tcttcgatgc cgttgccttg ggcctcctgc gcaagacgct catcgaaaca
    24241 ctcggcaccg atgcagcgcg cggtctcatg actcgctttg gatacgtgca tggccgtcgc
    24301 agcgcggaga acatgaagac ggcggtcccg tgggactccg atgacgattg gcgtcgcgcc
    24361 ggagggcgtc tgcacaagct gcagggactt gtcgtgcccg caccggtggg ttcggcgcgc
    24421 gcgacgacgc cggcaccgtt cgtcgaggat ctttggcagg actcgtacga agccgagcag
    24481 cacctgatcc acctcggccg atccgacgag cccgtgtgct ggagtcagtg cgggttcgct
    24541 agtggctatc tcagctacgc aaacggtcga gagatctact gcatcgaggt ccgctgccgc
    24601 ggtcgtggcg acgcggtctg ccacatggtc tgcagaccac gcgaggactg ggacgacaag
    24661 cacgcgcccg acctcgcgta cttcgagatg ccacgcctcg acgcgatgct cacacaggtg
    24721 acgaagaagt tgcggagtgc ggagcggcag ctgcgcacgc gaaagcagga gctcgcctcg
    24781 tatcgcgccg aaccaggcat gacgaagagc gcggcgatga acaaggtgct cgagctcgtc
    24841 gagcgtgtcg cgcaggtcga ttccacagtg ctcgtcaccg gcgagagtgg tgtcggcaag
    24901 gagcggatcg cgcgcatgat ccacgagcgc tccgcgcgtg cacagcgtgc tctcgtggcg
    24961 atcaactgcg gcgcgctgcc cgagaacttg ctcgagagcg agctgttcgg acacgccaag
    25021 ggatccttca ctggcgcgac acaggaccga gccgggctct tcgaagaggc gagcggcggc
    25081 acgttgttcc tcgatgagat cggtgaggtc acacccgcac tccaggtgaa gctcttgcgt
    25141 gcgctccagg agcacgagat ccggcgcgtc ggcgagaaca agaaccgcac cgtggacgtg
    25201 cgggtgatcg ctgcgacgaa tcgcaatctc accgaggagg tcaaggctgg acggtttcgg
    25261 caggacctct actaccggtt gcgcgtgata gagattgccg tgccgccgct tcggcagcgc
    25321 cgcgaagaca tcctgccgct tgcacgtgag ttgctcgcag cggctgcgac ccgcacgaaa
    25381 tcgaaggcga cgtcgttcac gcctgccgtg atcgatcagc tccagcgcta tgcatggccg
    25441 ggaaacgtgc gcgagctcga gaacgcactg gagcgcgccg cggtgatggc gcgcggtacg
    25501 cggatcgacg tcgcagactt gcctgccgag atcgcgggcg ccatcgcgag ctcgtggacg
    25561 cccggcgacg agcgctcgct caagcaggtc gagaaggatt acaccctcgc ggtgctcgaa
    25621 gcgaacggcg gcaaccgcac caagacggcg gcgcagctgg aaatcgcacc ggccacgctc
    25681 taccgcaagc tcgccgagta cggcgtgacc ggcaagtagt ccacgtcgat cgcgtgcact
    25741 ccgctacatg ttcgtctgat cgggcgggac gacgtttgga tccggaagcg ggtccgcgat
    25801 cggagactgc ttcgaatccc cgcccgggca ctcgcaatcg agcgtgtacg acgtcatcga
    25861 aatggatccg agtgcgtaac cctcgacgag gatgcgtggg gtgcgcttgc agtgtttcgc
    25921 gagaccggcg aggtagagca gcggaatgaa gtgatcgggc
//
LOCUS       DATLBZ010000224         6011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_970340, whole genome shotgun sequence.
ACCESSION   DATLBZ010000224 DATLBZ010000000
VERSION     DATLBZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970340"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VMZ53_11910"
     CDS             <1..1187
                     /locus_tag="VMZ53_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29213.1"
                     /translation="LKPGNILLGRRGEVYVIDWGIARELGTPDAFAGFILGTRGYMAP
                     EQERGDADLDERVDIYALGCILIDILECRATSAPPPELVKACQRATNSERDERFRTVA
                     ELGDIVQRYLDGDRDVEQRRALARSHLASARAAFATVSEDPERRATVMREAGKALALD
                     PTLDGAAELIGRLMLEPPAVKPREVERELIELDMRSAKLMSRVAVAVSSIYLAMLPLY
                     VIRGITDVPYLSAYGGLASFMLAIALVNRCRPRRALIITHVIAMLIMFALLSRMFTPL
                     AISPAIAAVTLVALSFNPLLGGRTWSIIVTLGTIVSVLGMWCAEWVGLLPPSVVHEHG
                     VLRLISPVDGVASFPPTPILPAMYALVCAIAGTVGYLATRQIRAARDKLVLQAWHLRQ
                     LV"
     gene            1272..3038
                     /locus_tag="VMZ53_11915"
     CDS             1272..3038
                     /locus_tag="VMZ53_11915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29214.1"
                     /translation="MRISAGVTVPLGHDDSLEPVTDPEVITSAEPQFARGSEPFTRVD
                     AAATRILTGEPRTRPASEPSVEPERYDLGGVIGRGGMGEVVAARDVQIGRDVAIKRLH
                     AAKPSEAQLARFLREARIQGRLEHPAIPPVHEIAHAEDGRPYFAMRKLEGVTLADTLR
                     DPKSPFTRQRLLRAFVDICHAIDLAHSRRIVHRDIKPSNILLGPRGEVFVLDWGIARE
                     LDAPDAFAGAVLGTPGYMAPEQARGDADLDERVDIFALGRLLSEILRRTNADPPVELT
                     AACERAMQENRACRFSTVAELCDVVQRFLDGDRDLEQRRALARHHLTAARDAFLQRRD
                     DGERRTIVMHEAGKALALDPTLNGAAELIGRIMLEPPTSKPAAVERELTELDLRGAKL
                     MSRVAIAASLAFVSMLPMFVLLGIHDVPYLLAYAGVSLGMLGIAIAGRRAPRRPLIAA
                     HIASMMVMFALLARMFSPLLISPALAAVTLVTISFNPLSSGRTTSIITTIGTAVSVLG
                     VWSLEWIGVLPSSVVRVPSGLLLLTPVEGAASFPAAPVLSGIYLLVFCMAGAVGYFAQ
                     REIRLAREKLVLQAWHLRQLVP"
     gene            3035..4900
                     /locus_tag="VMZ53_11920"
     CDS             3035..4900
                     /locus_tag="VMZ53_11920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29215.1"
                     /translation="MTTSSTHGVTIARDDASVEVTDVARPCEDIGPLFGAEGTPPQSR
                     RIATEMPAVRPETEETAVAAVVDPRGARRASAPLIPGGERYELGGEIGRGGMGEVLTA
                     RDVQIGREVAIKRLHLARSTNAAEARFLREARIQGRLEHPAIPPVYELAEDAEGRPYF
                     AMRKLEGVTLAATLRDASSPFTQQALLRAFVDICNAVDLAHSRRIIHRDLKPANILLG
                     ERGEVYVLDWGIARELDAPDAFSGAVIGTPGYMAPEQLRGERDLDERVDIYALGCMLY
                     EIVTRRPLHAKGDAGIEQALRGEFPYDAQIPLELSNACRCATAVQRSQRTSSARELAQ
                     TVQRYLDGDRDLEQRRTLAQHHFASARAAFETVRDDTDRRSIVMREAGRALALDPTLS
                     GAAELIGHLMLRAPATIPAAVHQEIDAIDVTSARLMIKLSQRTTVLYLLMLGVFIACG
                     VRDVLYLCAYGGVATLLHALTIVGLRRPHVFLGPIFGMGLVALVAVLARMFTPVLVAP
                     SIAAVVVGSTVFNPTMKRRDVIILTAGMLLAIGGVWIAELVGLVSETMTRTGDTVHLR
                     MPVEGISSFPIFASLAFSSTAIILVSALSSWIAMREIRVGREKLVMQAWHLRQLV"
     gene            complement(4901..5530)
                     /locus_tag="VMZ53_11925"
     CDS             complement(4901..5530)
                     /locus_tag="VMZ53_11925"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HUS29216.1"
                     /translation="MATVVIKKYGNRRLYDTGDSRYVTLDELAAKIRTGVDLRIVDAQ
                     TNEDLTQATLTQIVLETGHASKFLPVQLLTQMIRLSDDSLAEFFSRYVTGALDMYLQA
                     KRGVQAITTYNPLAQIPMAATDALTRMWMGSAFGGGPQYGAPPPQYGYPAPPPYPVAS
                     PPAAPEDETEEAPASNGDSRDDVAAMRRELDELKQAVRESLPKKKKKKS"
     gene            5635..>6011
                     /locus_tag="VMZ53_11930"
     CDS             5635..>6011
                     /locus_tag="VMZ53_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29217.1"
                     /translation="MTSRRALQFLLLCSIGVAAGGGCSGPAGETCSVDEDCASHFCRA
                     DGTCGPAPVDGAMDTGDAGIDSPSGLCAPNHDGQITLGELPLIAGRSATFRVATGATW
                     NTAGTSNTNGTHTWNLTGALAGDA"
BASE COUNT          935 a         2086 c         1953 g         1037 t
ORIGIN      
        1 atttgaagcc tggaaatatc ctgctcggcc gccgcggcga ggtctacgtc atcgactggg
       61 gcatcgcacg cgagctcggc acacccgacg cattcgcggg attcatcctc gggacgcgcg
      121 gatacatggc tcccgagcaa gagcgcggcg acgcggatct cgacgagcgt gtcgacatct
      181 acgctctggg ctgcatcctg atcgacatcc tcgagtgccg cgcgacgagc gcgccgccgc
      241 cggagctcgt aaaagcatgc cagcgcgcaa cgaacagcga gcgcgacgag cgattccgaa
      301 ccgtcgccga gctcggcgat atcgtgcagc gctacctcga cggcgaccgc gacgtcgagc
      361 agcggcgcgc gctcgcccgc agtcacctcg catcggcgcg ggcagccttc gcgacggtca
      421 gcgaggaccc cgaacgtcgc gcgactgtca tgcgagaagc cggcaaagcg ctcgcactcg
      481 acccgacgct cgacggcgcg gctgagctca tcggccgtct catgctcgaa ccacccgccg
      541 tgaagccacg cgaggtcgag cgcgagctca tcgagctcga tatgcgcagc gcaaagctga
      601 tgtcgcgtgt cgcggtcgct gtgtcgagca tctacctcgc gatgctgccg ctctacgtga
      661 tcagaggcat caccgacgtg ccttatctgt cggcgtacgg cggcctcgcg tcattcatgc
      721 tcgcgatcgc gctcgtgaac cgatgcagac cgcgccgcgc gttgatcatt acgcacgtca
      781 tcgcaatgtt gatcatgttc gcgctgctat cgcgcatgtt cactccactg gcgatttcac
      841 cggcgatcgc cgccgtcaca ctcgtcgcgc tctcgttcaa tccgctgctc ggcgggagaa
      901 cctggtcgat catcgtgacg ctcggcacga tcgtctccgt gctcggcatg tggtgcgccg
      961 agtgggtcgg actgcttccg ccatcggtcg ttcacgagca cggggtgctg cggctcatca
     1021 gccccgtgga tggggtcgcg agctttccgc cgacgccaat cctgccggca atgtacgcac
     1081 tcgtctgcgc aatcgccgga acggtcggct acctcgcgac gcgtcagatc cgggcagccc
     1141 gcgacaagct cgtgctccag gcctggcacc tgcggcagct tgtctgaagg ccttgggccc
     1201 cagccgcgcg cggctaatgc cgtggattaa tgggcgccgc gacaggcacc ccggttgcac
     1261 atcatgccgt cgtgcgcatc tccgcgggcg tgacggtgcc cctgggtcac gacgacagcc
     1321 tcgagcccgt cactgatcca gaggtgatca cgagcgccga gccccagttc gcccgaggca
     1381 gtgagccgtt cacccgcgtc gatgctgcgg cgacccgcat cctcacgggc gagccccgta
     1441 cccggcctgc ctcggagccc agcgtcgagc cggagcgtta cgacctcggc ggcgtgatcg
     1501 gccgcggcgg catgggcgag gtcgtggctg cgcgcgatgt ccagatcggc cgcgacgtcg
     1561 ccatcaagcg gctgcacgcg gcaaagccca gcgaggcgca gctcgcgcgg tttctgcgcg
     1621 aggcccgcat ccaaggccgc ctcgagcatc ctgcaattcc gcccgtgcac gagatcgcgc
     1681 acgctgaaga cggccggccg tacttcgcga tgcgcaagct cgaaggcgtc acgctcgcgg
     1741 acacgctgcg cgatccgaag tcgccgttca ctcgccagcg cttgctgcgc gcattcgtcg
     1801 acatctgtca cgcgatcgat ctcgcgcact cgcgccgcat cgttcaccgc gacatcaaac
     1861 cctcgaacat cctgctcggt cctcgcggcg aggtattcgt gctcgattgg ggcatcgcac
     1921 gcgagctcga cgcacccgac gcgtttgcgg gcgccgtcct cggcacgccc ggctacatgg
     1981 cgccggagca agcacgcggt gacgccgacc tcgacgagcg cgtcgacatc ttcgcactcg
     2041 gtcgcctgct gtccgagatc ctgcggcgca cgaatgccga cccgccggtg gagctcaccg
     2101 ccgcctgcga gcgagcgatg caggagaatc gcgcgtgccg gttctcgacg gtggccgagc
     2161 tgtgcgacgt cgttcagcgc ttcctcgacg gcgatcgcga tctcgaacag cgccgcgcgc
     2221 tcgcacgcca ccacctcacc gcggctcgcg atgcctttct gcagcgccgc gacgatggcg
     2281 aacgccgcac gatcgtcatg cacgaggcag gcaaggcgct cgcactcgat cccactctga
     2341 acggcgcagc cgagctcatc ggtcgcatca tgctcgagcc gccgacgtcg aagccggccg
     2401 cggtcgaacg cgagctcacc gagcttgatc tgcgcggggc gaagctgatg tcgcgcgtcg
     2461 cgatcgcggc atcactcgcg tttgtctcga tgttgccgat gttcgtcctg ctcggcattc
     2521 acgacgtgcc gtacctgctc gcctacgcgg gcgtttcgct cggcatgctc ggcatcgcga
     2581 tcgcaggacg gcgcgcgcca cgccggccgc tcattgccgc gcacatcgcg tcgatgatgg
     2641 tgatgttcgc gctgctcgcg cggatgttct cgccgctgct gatctcgccg gcgctcgcag
     2701 cggtcacgct cgtgacgatc tcgttcaatc cactgtcgag tggacgcacg acctcgatca
     2761 tcacgacaat cggcaccgcg gtctccgtgc tcggcgtgtg gagcctcgag tggattggag
     2821 tcctaccgtc gtcggtcgtg cgcgtgccgt ccggcctgtt acttctgaca cccgttgaag
     2881 gcgcggcgag ctttccagcg gcacccgtcc tctcgggcat ctacttgctc gtgttctgta
     2941 tggccggcgc ggtcggctat ttcgcgcagc gcgagatccg gctcgctcgc gagaagctcg
     3001 tgctgcaggc gtggcacctg aggcagctag ttccatgacg acgagctcga cccacggcgt
     3061 aacgatcgcg cgagacgacg catccgtcga agtcacagac gtcgcgcgtc catgcgagga
     3121 catcggcccc ttgttcggcg ccgaaggcac gccgccgcag tcacgccgga tcgcgaccga
     3181 gatgccggcg gttcgcccag agaccgagga gactgccgtc gcggctgtcg tcgatccgcg
     3241 tggcgccagg cgcgcgtctg ctcctctgat tccaggcggg gagcgctacg agctcggcgg
     3301 cgaaatcggc cgcggcggca tgggcgaagt gctcaccgcg cgcgatgttc aaatcggccg
     3361 cgaggtcgcg atcaaacggc ttcatctcgc gcgctccacg aatgcagcgg aagcgaggtt
     3421 tctgcgcgag gcgcgcattc aaggccgcct cgagcatccc gcgattccac cggtctacga
     3481 gctcgccgaa gacgccgaag gccgacccta cttcgcgatg cgcaagctcg aaggcgtcac
     3541 actcgcggca acgctgcgcg atgcgagctc gccgttcacg caacaagcgc tgctgcgtgc
     3601 cttcgtcgac atctgcaacg cggtcgatct cgcgcactcg cgccgcatca tccaccgcga
     3661 tctcaaaccg gcgaacatcc tgctcggcga gcgtggcgag gtgtacgtcc tcgactgggg
     3721 catcgcgcgc gagctcgacg cacccgacgc attttccggc gccgtcatag gaacgcccgg
     3781 ctacatggcg cccgaacagc tccgcggtga gcgagacctc gacgagcgcg tcgatatcta
     3841 cgcgctcggt tgcatgctct acgagatcgt gacgcgacgc ccacttcatg cgaagggcga
     3901 cgccggtatc gagcaagcat tgcgcggtga gtttccgtac gacgcgcaga taccgctcga
     3961 gctctcgaac gcgtgccggt gtgcgaccgc cgtgcagcgc agccagcgca cgagctcagc
     4021 gcgcgagctc gcgcagaccg tccagcgcta cctcgacgga gatcgcgatc tcgagcagcg
     4081 tcgcacgctc gcgcagcatc acttcgcatc ggcgcgcgcc gcattcgaga cggtccgcga
     4141 cgatacggac cgccgcagca tcgtcatgcg cgaagccggc agagccctcg cactcgaccc
     4201 gacgctctcg ggtgcagcgg agctcatcgg ccacctaatg cttcgggcac cggcgacgat
     4261 ccccgccgcg gtccaccagg agatcgacgc gatcgacgtc acgtcggcgc gcctgatgat
     4321 caagctctcg cagcggacca cggtgctcta cctcctcatg ctcggcgtgt tcatcgcgtg
     4381 cggcgtgcgc gacgtgctgt acctgtgcgc gtacggcgga gtcgccacct tgctgcatgc
     4441 gctcacgatc gtcggcctac gccggccaca cgtgttcttg ggcccgatct tcggtatggg
     4501 tctcgtcgca ctggtcgccg tcctcgcgcg catgttcacg ccggtgctcg tcgcaccttc
     4561 gatcgcggcc gtcgtcgtcg gctcgacggt gttcaacccc acgatgaagc gccgcgatgt
     4621 gatcatcctg accgcgggga tgctcctcgc gattggtggc gtctggatcg cagagctcgt
     4681 aggcctcgtg tcggagacga tgacccgcac cggcgacacg gtgcatctgc gcatgcccgt
     4741 cgagggcatc agcagcttcc ccatcttcgc gagcctcgcg ttctctagca ccgcgatcat
     4801 cctggtctcg gcgctatcga gctggatcgc gatgcgcgaa attcgagtcg gacgcgagaa
     4861 gctggtcatg caggcgtggc acctgcgcca gctcgtgtag ctacgacttc tttttcttct
     4921 tcttcggcag gctctcgcga actgcttgct tgagctcgtc gagctcgcgt cgcatcgcag
     4981 caacgtcgtc gcgcgaatcg ccattcgaag cgggtgcttc ttcggtctcg tcctcgggcg
     5041 cagcaggagg cgacgcgacg ggatatggcg gcggcgccgg atagccgtac tgcggcggcg
     5101 gagcgccgta ctgcgggccg ccaccgaagg cggagcccat ccacatccgc gtgagcgcat
     5161 cggtcgcggc catcgggatc tgcgcgagcg ggttgtacgt cgtgatcgcc tgcacgccgc
     5221 gcttggcctg caggtacatg tcgagggcgc ccgtgacata gcggctgaag aactcggcga
     5281 gcgagtcgtc ggacagccgg atcatctggg tgagtagctg gaccggcagg aacttcgagg
     5341 cgtggcccgt ctcgagcacg atctgagtca gcgtcgcctg ggtcaggtct tcgttggtct
     5401 gcgcgtcgac gatgcggagg tccactcccg tgcggatctt cgccgccagc tcgtcgaggg
     5461 tgacgtagcg gctatctccg gtgtcgtaga gacgccggtt cccgtatttc ttgatgacca
     5521 ccgtcgccac gggtcggacc ttactggggg cctcgctgca ctgcaagcag actttttgct
     5581 gcgccgcaaa aacgggcttg acgccggccc ggccacggtc gtagcgtgcc gcgcatgacg
     5641 tcccgccgcg cgctccaatt tttgctgctc tgcagcatag gagttgctgc aggcggcgga
     5701 tgtagcgggc cggccggcga gacgtgctcg gtcgatgaag attgcgcgtc gcacttctgc
     5761 cgcgccgatg gcacgtgcgg accggcaccg gtcgatggcg cgatggatac gggcgatgcc
     5821 ggcatcgatt cgccgagcgg cctgtgcgca ccgaaccacg atggccagat cacgctcggc
     5881 gagctgccgc tgatcgcggg gcgctctgcg acgtttcgcg tggcgaccgg cgcgacgtgg
     5941 aacaccgccg gcacctcgaa cacgaatggc acgcacacgt ggaatctcac cggcgcgctc
     6001 gcgggtgacg c
//
LOCUS       DATLBZ010000225         3850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6821099, whole genome shotgun sequence.
ACCESSION   DATLBZ010000225 DATLBZ010000000
VERSION     DATLBZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6821099"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VMZ53_11935"
     CDS             complement(<1..514)
                     /locus_tag="VMZ53_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29218.1"
                     /translation="MLALVQDPLLSRVDRRLAAILGGSIALHIAIAAYAWSTDIEEPA
                     PLATSAIPVEYQDIDILIPDFTPPQKVTSTAPGTAKPVAPNQTPTPIVRRQTIRPAIT
                     TGNDVDPAKLAGLFTNDDTAHRRPGMDLGKQIIDARNNHVEVGKDSAPDPSPRLGTQP
                     DLPLTDPTLTH"
     gene            596..1540
                     /gene="cysD"
                     /locus_tag="VMZ53_11940"
     CDS             596..1540
                     /gene="cysD"
                     /locus_tag="VMZ53_11940"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HUS29219.1"
                     /translation="MRTAPETDICLSLRTHRRTHLRTLEDEAVHILRELAAERERPCL
                     LFSGGKDSIVLLRLAEKAFRPARFPFPLVHVDTGHNFPETLVYRDRRVEELGERLIVA
                     SVQASIDAGRVSEEKGPRASRNRLQTTTLLDCIEEHQFDAAIGGARRDEERARAKERI
                     FSHRDEFGQWDPKNQRPELWSLYNGRVRKGEHLRVFPLSNWTELDVWEYIRLEDIPLP
                     SIYFAHEREVFERDGMLYAANPYVTLLPNERIRKEVVRFRTIGDMTCTGAVKSDARTL
                     DEVIAEVAAARITERGATRADDRFTEAAMEDRKREGYF"
     gene            1540..2850
                     /locus_tag="VMZ53_11945"
     CDS             1540..2850
                     /locus_tag="VMZ53_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUS29220.1"
                     /translation="MSAAALPAPSLATSGLADDSELLRFTTAGSVDDGKSTLIGRLLY
                     DTKTAFIDQLEHVEDVSRRRGDERVNLALLTDGLRAEREQGITIDVAYRYFATKTRKF
                     IIGDTPGHVQYTRNMVTGASTAHLAIVLVDARQGVVEQSRRHALIASLLRTPHLLFAI
                     NKMDLVGWSEARFREIEAELRAFASHLDVNDIAFIPISALTGANVVERSLEMPWHQGP
                     TLLEHLESVPITTDRDQFSARFPVQWVIRPQSDAHHDYRGYAGQVAGGVLRPGDEVTV
                     LPSGRTTTISRIEEGGEPIEAAFAPMSVTVHLADDLDVGRGDLICRRSNELPIVGQDL
                     EAMICWMTDKPLQARGRYALKHTTKWTRAIVSEIVHRFDVSTGEVDATATELPLNGLG
                     RIKLRTTNPLVYDPYAQNRTTGSFILVDESTNATVAAGMLLGDT"
     gene            2854..3492
                     /locus_tag="VMZ53_11950"
     CDS             2854..3492
                     /locus_tag="VMZ53_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29221.1"
                     /translation="MSGRNEQLGKQLRRLRTSAAIEDADACETALRRATHLVRDLVHE
                     HAEADEHDAALAELVSKRIVDAALAERIAACIERFDEESGVEPAGIEELLADGAVLDR
                     FLRSIEHGALMPELPFEAYVSVGGIAESATSKSALTVSGRHVLLAERGREGMVFIDDK
                     PVGRPFPLLAAPRTKPDVRHEATGVTRIVWEAENLVVELAADFTSASIIAAE"
     gene            3489..>3850
                     /locus_tag="VMZ53_11955"
     CDS             3489..>3850
                     /locus_tag="VMZ53_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29222.1"
                     /translation="MTGPNLNHIKIVDVTFGNNVTVLQPANMYGCILGDDVFIGPFVE
                     VQKEVTIGARTRVQSHSFICELVTIGEDCFIAHGVMFINDLFETGKPAKGRKELWKST
                     KVGNRVSIGSNATILPVE"
BASE COUNT          689 a         1214 c         1281 g          666 t
ORIGIN      
        1 catgcgtcag cgtcggatcg gtgagcggca ggtcgggctg cgtgccgaga cgcgggctgg
       61 gatccggcgc cgagtccttg ccgacttcga cgtgattgtt gcgtgcgtcg atgatctgct
      121 tgccgagatc catgcccggg cgccggtgcg cggtgtcgtc gttcgtgaag aggccggcga
      181 gctttgcggg atcgacgtca ttgccggtgg tgatcgcagg acggatcgtc tggcgacgta
      241 cgatcggagt tggcgtctga ttcggcgcga caggcttcgc ggtgcccggt gctgtgctcg
      301 tcaccttttg cggcggcgtg aagtcgggga tgaggatgtc gatgtcttgg tattcgaccg
      361 ggatcgcgga ggtcgcgagc ggcgcgggct cctcgatgtc cgtcgaccag gcgtaggcag
      421 cgatcgcgat gtggagcgcg atcgagcctc ccaaaatggc ggccagacgg cggtcgacgc
      481 ggctcagaag cgggtcctgg accagcgcca acatcgggtc attgcgtcgt aatctcgcca
      541 tgttcccttc aacgtccgag ggtcccgagc gatctaagcg gacgcatatc cggtattgcg
      601 gacggcgcca gaaacggata tatgtctgtc attgcggacg caccggcgaa cacacctgcg
      661 gaccctcgag gacgaggccg ttcacatcct gcgcgagctc gcggccgagc gtgagcgtcc
      721 gtgcttgctg ttctccggcg gcaaggactc gatcgttctg ctgcgcctcg ccgagaaggc
      781 attccggccc gcgcggtttc catttccgct cgtgcacgtc gataccggcc acaacttccc
      841 ggagacgctc gtgtaccgcg accgtcgcgt tgaagagctc ggcgagcggt tgatcgtcgc
      901 cagcgtccag gcatcgatcg atgcgggccg cgtgagtgaa gagaagggac cgcgcgcttc
      961 gcgcaatcgg ttgcagacga cgacgctgct cgactgcatc gaagagcatc agttcgacgc
     1021 tgcgatcggc ggagcgcggc gcgatgaaga aagagcgcgc gcgaaggagc gcatcttcag
     1081 ccaccgcgac gagttcggtc agtgggatcc gaagaaccag cggccagagc tgtggagcct
     1141 ctacaacggg cgcgttcgga agggcgagca cctgcgcgtg ttcccgctgt cgaattggac
     1201 cgagctcgac gtctgggaat acatccggct cgaagacatc ccgctgccgt ccatttactt
     1261 cgcgcacgag cgcgaggtgt tcgaacgcga tggaatgctc tacgccgcga acccgtacgt
     1321 gacgctcttg ccgaacgagc gcattcgcaa ggaggtcgtt cggtttcgga cgatcggcga
     1381 catgacgtgc accggcgcgg tgaagtccga tgcgcggaca ctcgacgagg tgatcgccga
     1441 agttgccgcg gcgcgaatta cggagcgtgg cgcgacgcgt gccgacgacc gcttcaccga
     1501 agctgccatg gaagaccgca agcgagaggg gtacttctga tgtctgccgc cgcgctaccc
     1561 gcgccatcgc tcgcaacctc aggtctcgcc gacgacagcg agctgcttcg cttcacgacc
     1621 gccggctccg tcgacgacgg caagtcgacg ctcatcggcc gcctgctcta cgacacgaag
     1681 acggcgttca tcgatcagct ggagcacgtc gaggacgtct cgcgccggcg tggcgacgag
     1741 cgcgtgaacc tcgcgctgct caccgatggc ctgcgcgccg agcgcgaaca aggcatcacg
     1801 atcgatgtcg cgtatcgcta cttcgcgacg aagacgcgca agttcatcat cggggacacg
     1861 ccgggccacg tgcagtacac gcgcaacatg gtgacgggcg cgtcgacggc gcatctcgcg
     1921 atcgtgctcg tcgatgcgcg gcaaggcgtc gtcgagcaat cgcgccgcca cgccctgatc
     1981 gcgtcgctgc tgcgcactcc tcacctgctg ttcgcgatca acaagatgga tctcgttggc
     2041 tggagcgagg cgcggtttcg cgagatcgag gcggagctcc gcgcgtttgc gtcgcacctc
     2101 gacgtcaacg acatcgcatt cattccgatc agcgcgctga cgggtgcgaa tgtcgtcgag
     2161 cgctcgctgg agatgccgtg gcaccagggc cctaccctcc tcgagcatct cgagagcgtt
     2221 ccgatcacga cagaccgcga ccaattctcc gcgcggttcc ccgtgcagtg ggtgattcgc
     2281 ccgcaatcgg acgcgcacca cgactaccgc ggctatgcgg ggcaagtcgc aggcggcgtg
     2341 ctgcgtcccg gcgacgaagt cacagtcctg ccgtcgggcc gcacgacgac gatctctcgt
     2401 atcgaagaag gcggcgagcc gatcgaggca gcgttcgcac cgatgagcgt caccgttcat
     2461 ctcgccgatg atctcgatgt cggccgcggc gacctgatct gcaggcgctc gaacgagctg
     2521 ccgatcgtcg gccaggatct cgaggccatg atctgctgga tgacggacaa gccgctgcaa
     2581 gcgcgaggtc gctacgcgct gaagcacacg acgaagtgga cgcgcgcgat cgtcagcgag
     2641 atcgtgcacc gcttcgacgt ctcgacgggc gaagtcgacg ccactgcgac ggagcttccg
     2701 ctcaacggcc tcggccgcat caagctgagg accacgaatc cgctcgtcta cgatccgtat
     2761 gcgcagaatc gcacgaccgg ctcgttcatc ctcgtcgacg aatcgacgaa cgcgaccgtt
     2821 gcggccggca tgcttctggg cgacacctaa cgggtgtcag gacgtaacga gcagctcggc
     2881 aaacagttgc gccggctccg cacgagcgct gcgatcgaag acgccgatgc gtgcgagacc
     2941 gcgctgcgcc gtgcaacgca tctcgttcga gacttggtcc acgagcacgc cgaagccgac
     3001 gagcacgatg cggcgctcgc cgagctcgtc agcaagcgca tcgtcgacgc agcactcgcc
     3061 gagcggatcg ctgcttgtat cgagcggttc gacgaggaga gcggcgtcga gccagcgggc
     3121 atcgaagagc tgctggccga cggagccgtc ctcgatcggt ttctgcgcag catcgagcac
     3181 ggtgcgttga tgcccgagct gccgttcgag gcgtatgtca gcgtcggtgg gatcgccgag
     3241 tcggccacgt cgaagtccgc cctgacggtc tcgggccggc acgtcttgct cgccgagcgt
     3301 ggccgcgaag gcatggtctt catcgacgac aaaccggtcg gcaggccgtt cccattgctg
     3361 gcagcaccgc ggaccaaacc cgatgtacgt cacgaggcga cgggcgtcac gcggatcgtg
     3421 tgggaagccg agaacctggt cgtcgagctt gcggcggact tcacgtcggc gtctatcatc
     3481 gcggctgaat gaccgggccc aatctcaacc acatcaagat tgtcgacgtg acgtttggga
     3541 acaacgtcac cgtccttcag cccgcgaaca tgtacggctg catcctcggc gatgacgtgt
     3601 tcatcggccc gttcgtcgaa gtgcagaagg aagtaacgat cggtgcgcgc acgcgtgtgc
     3661 aatcgcactc gttcatttgc gagctcgtca cgatcggcga ggattgcttc atcgcgcacg
     3721 gcgtgatgtt catcaacgat ctcttcgaga ccggcaagcc ggcgaagggc cgcaaggagc
     3781 tctggaaatc gacgaaggtc ggcaatcgcg tgagcatcgg ctcgaacgcg acgattcttc
     3841 ccgtcgagat
//
LOCUS       DATLBZ010000226         8572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7567356, whole genome shotgun sequence.
ACCESSION   DATLBZ010000226 DATLBZ010000000
VERSION     DATLBZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7567356"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VMZ53_11960"
     CDS             complement(<1..1213)
                     /locus_tag="VMZ53_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HUS29223.1"
                     /translation="MAGFDHKNIDQKWQQYWDKEQTFLTPTDRTKPKYYVLDMFPYPS
                     GDGLHVGHPKGYTATDVVARAKRMMGFNVLRVMGWDSFGLPAERRAERTGEHPSVITA
                     RNIATFKSQLKKLGLSYDWTRELATSEPKYYKWTQWIFEVLAQRGLAYRSEVPVNFCP
                     ALGTVLANEEVKDGKYIETGDDVEKRLMTQWMLKITQYADRLSEDLAGVDWPHGTLVA
                     QRDWIGKSIGANVTFKTDADDIVVFTTRPDTLFGGTYVVLAPEHPLVDKLTVAAQRDA
                     VAQYREQVSKRSERDRTTEAADAPKTGVPTGAFAVNPVNGKKIPIWVADYVLVSYGTG
                     AVFACPAHDERDHAFAKKFNLPIIEVVEGGNVDEAAFTGDGPHVNSAFLDGLDIAAAK
                     DKVIAWLAERSL"
     gene            complement(1213..1842)
                     /locus_tag="VMZ53_11965"
     CDS             complement(1213..1842)
                     /locus_tag="VMZ53_11965"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HUS29224.1"
                     /translation="MRMILVGPPGAGKGTQAARLVEQFRIPHISSGDMFRAEVKSGSE
                     LGKELDRHMKTGGLVPDEVTIPIVLKRITSPDCANGFLLDGFPRTRPQAEALDREMTK
                     ANVALDAVVLIEVPDQLLEERAVMRRSDPETGTIYHLKYNPPPEAILARLVHRADDTK
                     EAVDKRIAKYHSETAPIVPFYKEKGILKNVDGVGDPNAITDRIKSVLKS"
     gene            1909..2283
                     /locus_tag="VMZ53_11970"
     CDS             1909..2283
                     /locus_tag="VMZ53_11970"
                     /inference="COORDINATES: protein motif:HMM:NF026420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane 220 family protein"
                     /protein_id="HUS29225.1"
                     /translation="MKPVPRWFLALCWAMAVLFSLCVGLQANDPDPARWMALYGAAAL
                     ACAVLPARRFVAIGAAVIGLIAAAWGAYLAHQVIDVLSFSDLFMKMDEKGGAVEVGRE
                     AGGLAIVAAMMLGGAGFRAMRA"
     gene            complement(2307..2744)
                     /locus_tag="VMZ53_11975"
     CDS             complement(2307..2744)
                     /locus_tag="VMZ53_11975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS29226.1"
                     /translation="METTWLLRTFDELTAAQLYAIVELRERVFVVEQRCVYLDADGLD
                     RSARHLWAERGGAMVAYLRILPAGLKYKEVAIGRVVTARAARGGGLGRELVRRGIDAC
                     GHVPIRISAQAHLEEWYRQLGFKRASEIYDEDGIAHIDMLRTP"
     gene            2767..3303
                     /locus_tag="VMZ53_11980"
     CDS             2767..3303
                     /locus_tag="VMZ53_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HUS29227.1"
                     /translation="MRQVCVYLSSSVGLPSYVAMIDELGRELANRDLTLVYGGAHRGL
                     MGVLADSVLGAGGRALGVIPQALVEAEVAHTGLTKLFVVETMHQRKAMMFDLADAFIV
                     APGGFGTLEEAFEMLTGLQLGYHAKPIVFLDQDAFWAPLGAFLDHAVTQGVLQPSVRA
                     LFRTAPTPAAALDLLGGR"
     gene            3343..5280
                     /locus_tag="VMZ53_11985"
     CDS             3343..5280
                     /locus_tag="VMZ53_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29228.1"
                     /translation="MAPRALGALFALVAAIAFAASIATSAWWSGPPTVDGDLREVQNV
                     HVGLLGGEGCNPGGEGGCETVEVSDTLRLMSYGVLGVHALATLFALLLAISAARVGDS
                     RKGLATTSLIMTLLAAGGAAAMLVLGPAIQAGSSVVEVPFGYGLYILGGAILSSFIAT
                     LLVRRIEPEPLRLKPGLAPTAQHAPPDIRDILRSDHEGVRPSAPNLFDGAPQLRPLYD
                     AEGVPPAPTAPNVPTRAPTPLPQRQIDVLTGKATPPPLARPAPDAFAQTMPAPAIMQP
                     PPPRAKPVTSPPRTKPPSAAPPAAKVDLAGPTLNAPKQPRAKTLPAGSDPGIKRPTQP
                     PPSPAASQPMLRSSAPTLAHSVPPMPTLDNQPPPAVLPAGQRATTETDDSLETGMRPT
                     ENITAVEIDHEAKAKAQHARITGQRPAPSTKPPAIKSPPIKPLVPAPAKGAKPVPPQR
                     HDTEVATGDEAALGDVTETGMEAAPPRPGNIITGEQRSLSTVQREVARAPTDDPFDAR
                     EVTMPPRSHHDPVHAPISETFPVARISELVPASIVPDEGADDGPEDLMATMQRDKTPI
                     PTLNSREQNRMPSETPAPGTQLPSPRSMSTAPSSLPPPKNLTVDTVPSGPTPACPQCE
                     SPMAWVEEHLRFYCKSCRMYF"
     gene            complement(5277..7271)
                     /locus_tag="VMZ53_11990"
     CDS             complement(5277..7271)
                     /locus_tag="VMZ53_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29229.1"
                     /translation="MKLARVCLLAVLVSCGPSTGGDDDGTGTPGDADGDLISDADEGQ
                     ATLSDTDHDGTPDFQDDDSDGDGIPDYREAGDSDPDSAPLDSDGDGTPDFRDLDSDNN
                     ERSDTLDGLDDLDNDSVANFQDNDDDGDQIPDTGELGPNPQQALDTDADGTPDFHDLD
                     SDADTITDVLETNEDYDGDGGGNFIDTDSDGDCVPDKIEAGGSPPVDTDADHHYDFID
                     RDSDDDGVADVTEDANCNGVRDGGETNSKASDSDADGVSDLIEIAAGTNPNNATSNPQ
                     ANGDFVFVEPYMKTQSPVDDDLDFSTKLQAVDMYVLIDRSGSMSQEIVSVRDNLATAI
                     AHLTCGGLGSNPATCIPDLWAGAGTIGYHGSGAAAFQNYKDIQKNPNISGIPVSEPTG
                     TDSMEPLTFAAYAAVTGQGGANFTMGSVPARGTCAGSPAALNNYGTFGYPCFRQGALP
                     VILLATDEPAISAGDTYTSPGWSATVKQSFLNAKAKLVGIMGSGVAGTSVETDMRTMA
                     ADTGAVDAANGNAPLVFDGSGANAATAIENGIRTLASGLPLDINAIGVDDPTDSVNAI
                     TSFVDHLQTLQLGTAACANGLTDTDTNADGFRDKYLHVRTGTPVCWKVVSKQNTTVPA
                     KDVPQLFKAKVKLYGDGVTQLDDRNVFFLVPPQPLDNPIF"
     gene            7408..8100
                     /locus_tag="VMZ53_11995"
     CDS             7408..8100
                     /locus_tag="VMZ53_11995"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HUS29230.1"
                     /translation="MGSLLLIRHGQAAYGEADHDRLTPRGVQQAQALGHFLATARIDR
                     VYVGPHRRHQQTFDHAHATKPLPEPTLLPGLAEYPAVELLTHFMPKLIAEDPRFAELP
                     RTPTRELAGRALHTILGRWARDEWQVDGVERVEKFAERVRGALDQIIAEAKSGARIAV
                     FTSAGPIGVSAGLTFGATPHHMVRSSIVIRNTSVSEIRFRSDDFAWHPERIALVSFNS
                     THHLPPELHTEY"
     gene            8102..>8572
                     /locus_tag="VMZ53_12000"
     CDS             8102..>8572
                     /locus_tag="VMZ53_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS29231.1"
                     /translation="MNFDPPESVRPLLAKYEQFISEVVMPAEAVVLERGFKEAAPQLL
                     ALRARAKQEGLWLPQMDKHAGGLGLSLVEHGLVSERLGRSPLGHYVCNAQAPDAGNME
                     ILHKYGTPDQKKRWLEPLTSGEIRSCFSMTEPENPGSNPTLLSCTAKRDGDHYVI"
BASE COUNT         1316 a         2895 c         2826 g         1535 t
ORIGIN      
        1 cgagcgatcg ctccgcgagc cacgcgatga ccttgtcctt cgccgcggcg atgtcgagcc
       61 cgtcgaggaa cgcgctgttg acgtgcggtc catcgcccgt gaacgctgcc tcgtcgacgt
      121 tgccaccctc cacgacctcg atgatcggca gattgaactt cttcgcgaat gcatgatcgc
      181 gctcgtcgtg cgcggggcac gcgaacaccg caccggtgcc gtagctgacg agcacgtagt
      241 cggcgaccca gatcggaatc ttcttcccgt tgaccggatt caccgcgaat gcgcccgtcg
      301 gcacgccggt cttgggcgcg tctgccgcct cggtcgtgcg atcgcgttcg ctgcgcttcg
      361 acacctgctc gcggtattgc gcgacggcat cgcgctgcgc cgcgacggtc agcttgtcga
      421 cgagcggatg ctccggtgcg agcacgacgt acgtaccgcc gaacagcgtg tccggacgcg
      481 tcgtgaacac gacgatgtca tcggcatcgg tcttgaacgt gacgttcgcg ccgatcgact
      541 tgccgatcca atcgcgctgc gcgacgagcg tgccgtgcgg ccagtcgacg ccggcgagat
      601 cttcgctgag gcgatccgcg tactgcgtga tcttcagcat ccactgcgtc atcaggcgct
      661 tctcgacgtc gtcgcccgtc tcgatgtact tgccgtcttt gacttcctcg ttcgcgagca
      721 cggtgccgag cgcggggcag aagttgacgg gcacttccga gcgatatgcg aggccgcgct
      781 gcgcgagcac ctcgaagatc cactgcgtcc acttgtagta cttcggctcg ctcgtcgcga
      841 gctcgcgtgt ccagtcgtac gagagcccca gcttcttcag ctggctcttg aacgtggcga
      901 tgttgcgcgc cgtgatgacg ctcgggtgct cgcccgttcg ctcggcgcga cgctcggccg
      961 gcagaccgaa cgagtcccag cccatcacgc gcagcacgtt gaagcccatc atgcgcttcg
     1021 cacgcgcgac gacgtccgtc gccgtgtagc ccttcggatg gccgacgtgg aggccgtcac
     1081 ccgacgggta cgggaacatg tcgagcacgt agtacttcgg cttggtgcga tccgtcgggg
     1141 tcaggaacgt ctgttccttg tcccagtact gctgccactt ctgatcgata tttttgtggt
     1201 cgaagcccgc cattaggatt tcagtaccga cttgatgcga tcggtaatcg cgttcgggtc
     1261 gccgacgccg tcgacgttct tgaggattcc cttctcttta tagaagggaa cgatcggcgc
     1321 ggtctccgaa tgatacttcg cgatccgctt gtcgacagct tccttcgtgt cgtcggcgcg
     1381 atgcacgaga cgcgcgagga tggcctcggg tggcgggttg tacttgaggt ggtagatcgt
     1441 tcccgtttcg gggtcgctgc gacgcatcac cgcgcgctct tcgagcagct ggtcagggac
     1501 ctcgatcagg acgaccgcgt cgagggcgac gttggccttc gtcatctcgc gatcgagcgc
     1561 ttcggcctgt ggacgcgtgc gcgggaagcc gtcgagcaaa aagccgttcg cgcaatcggg
     1621 cgaggtgatg cgcttgagca cgatcgggat cgtgacttca tcgggcacga ggccgccggt
     1681 cttcatgtgg cgatcgagct ctttgccgag ctcactgccg gacttgacct cggcgcgaaa
     1741 catgtcgccg ctcgagatgt gcggaatgcg aaactgttcg acgaggcgtg ccgcctgcgt
     1801 gcctttgcca gctcccggcg gaccgacgag gatcatgcgc atgagcggca tccaagcatg
     1861 tgcgcgccgc ttctgcaatg caggccagct ctgcgttatg gtggatccat gaagccggtg
     1921 ccgagatggt tccttgccct gtgttgggcg atggccgtct tgttctcact gtgcgtcggc
     1981 ttgcaggcga acgaccccga tccggcgcgc tggatggcgc tctatggtgc ggctgcgctc
     2041 gcgtgtgcgg tgctcccggc gcggcgattc gtcgcgatcg gcgcagcggt catcggtctc
     2101 atcgccgccg catggggcgc gtatctcgct catcaagtca tcgacgtcct gtcgttctcg
     2161 gacctcttca tgaagatgga cgagaaaggc ggcgccgtcg aagtcggccg tgaagccggc
     2221 ggcctcgcga tcgtcgcggc aatgatgctc ggcggcgcgg gcttccgcgc gatgcgcgcg
     2281 tagcggctca ctcgcgcgat gcgcgctcaa ggcgtccgca gcatgtcgat gtgcgcgatg
     2341 ccgtcctcgt cgtagatctc cgacgcgcgc ttgaagccga gctgccggta ccactcttcg
     2401 aggtgcgcct gcgcgctgat ccggatcggc acgtggccgc acgcatcgat gccgcgccgt
     2461 acgagctcgc ggccaagccc gccgccccgc gccgcgcgag ctgtgaccac gcggccgatc
     2521 gcgacctctt tgtacttcag ccccgcgggc aggatgcgca aatacgcgac catcgccccg
     2581 ccgcgctcgg cccataaatg acgcgcgctg cggtcgagcc cgtccgcgtc gaggtacacg
     2641 cagcgctgct cgacgacgaa cacgcgctcg cggagctcga cgatcgcgta gagctgcgca
     2701 gcggtcagct cgtcaaacgt gcgcagaagc cacgtcgtct ccatgacctg atagtaaggt
     2761 acgcacgtgc gccaggtctg cgtatacctg tcatcgtcgg tggggttgcc atcgtatgtg
     2821 gcgatgatcg atgagctcgg acgggagctt gccaatcgcg acctcacgct cgtgtatggc
     2881 ggcgcgcatc gcggcctgat gggcgtgctc gcggattcgg tcctcggtgc cggtggtcgc
     2941 gcgctcggcg tcatcccgca ggcgctcgtc gaagccgagg tcgcgcatac gggtttgacg
     3001 aagctgttcg tcgtcgagac catgcatcag cgcaaggcga tgatgttcga cctcgccgat
     3061 gcgttcatcg tcgcgccggg cggcttcggc acgctcgaag aggcgttcga gatgctcacc
     3121 ggcttgcagc tcggctatca cgccaagccg atcgtgttcc tcgaccagga cgcattctgg
     3181 gcgccgctcg gtgcgtttct cgatcacgcc gtgacgcagg gcgtgcttca gccgtctgtc
     3241 cgcgcgctat tccgcacggc gccaacgccc gcagctgcgc tcgacctgct cggcggccgc
     3301 tagcttttcc gaccgcagcg gggtgcgtgg tagccttcgg tcatggcccc acgcgcgctc
     3361 ggcgcgctct tcgcgctagt ggccgcgatc gcgttcgccg cgtcgatcgc gacatcggcg
     3421 tggtggtctg gaccgccgac cgtcgatggt gatctgcgcg aagtgcagaa cgtccacgtc
     3481 ggcctgctcg gtggcgaggg ctgtaacccg ggcggcgaag gcggctgcga gacggtcgag
     3541 gtcagcgaca cgctgcggct catgagttac ggcgtcctcg gcgtgcatgc gctcgcgacg
     3601 ttgttcgcgc tgctgctcgc gatcagtgcg gcgcgcgtcg gtgactctcg caagggcctc
     3661 gcgacgacgt cgctgatcat gacgctgctc gctgcaggcg gtgcagctgc gatgctcgtg
     3721 ctcggacctg cgatccaagc gggcagttcc gtcgtcgagg tgccgttcgg gtacggcctc
     3781 tacatcctcg gcggcgcgat cctgtcgtcg ttcatcgcga cgctgctcgt ccgccgcatc
     3841 gagccggagc cacttcgcct caagccgggg ctcgcaccga cggcgcagca cgcgccgccc
     3901 gacatacgcg acatcctgcg cagcgaccac gaaggtgttc gaccgagcgc accgaatctg
     3961 ttcgacggtg cgccgcaact gcggccgctc tacgacgccg aaggtgttcc gcccgcaccc
     4021 acggcaccga acgttccgac gcgcgcgccg acgccgctgc cacaacgcca gatcgatgtg
     4081 ctcaccggca aggccacacc gccgccgctc gcaaggcccg cgcccgacgc gttcgcgcag
     4141 acgatgcccg cgcccgcgat catgcagcct ccgccgccgc gcgctaagcc ggtcacgtcg
     4201 ccgccgcgca cgaagccacc gagcgccgca ccaccggcag cgaaggtcga cctcgccggc
     4261 ccgacgctca acgcgccgaa gcagccgcgc gcgaagacgc tccctgcggg cagcgatccg
     4321 ggcatcaagc gcccgacgca gccacctccc tcgcccgccg catcacagcc gatgcttcgt
     4381 tcatctgcgc cgacgctcgc gcactccgtg ccgccgatgc cgacgctcga caatcagccg
     4441 ccaccggccg tgctaccagc cggccaacgc gcgacgacgg agaccgacga ctcactcgag
     4501 acgggcatgc gcccgaccga gaacatcacc gcggtcgaga tcgatcacga ggccaaagcg
     4561 aaagcgcagc acgcgcggat caccggtcaa cgccccgcgc cgtcgacgaa gccgcctgcg
     4621 atcaaatcgc cgccgatcaa gccgctcgtc cccgcgcccg ccaaaggagc gaagcccgtc
     4681 cctccgcagc ggcacgacac cgaagtagca acgggcgacg aagccgcgct cggagacgtc
     4741 accgagaccg gcatggaagc tgcgccgccg cgaccgggca acatcatcac gggcgagcag
     4801 cgatcgctgt cgacggtgca acgcgaagtc gcgcgcgctc ccaccgacga tccattcgac
     4861 gctcgcgaag tgacgatgcc accgcgctcg caccacgacc ccgttcacgc gccgatcagc
     4921 gagacgttcc cggtcgcgcg gatctccgag ctcgtgcccg cctcgatcgt ccccgacgaa
     4981 ggcgccgacg atgggcccga agatttgatg gcgacgatgc aacgcgacaa gacgccgatc
     5041 ccgacgctca actcgcgcga gcaaaaccgc atgccgtcgg agacgcccgc gcccggcacg
     5101 cagctgccat cgccgcgctc gatgtcgacc gcgccgtcgt cgctaccacc gccgaagaat
     5161 ctcaccgtcg acaccgtgcc gagtggaccg acgcctgcgt gcccgcagtg cgaatcgccg
     5221 atggcgtggg tcgaggagca cttgcgcttc tactgcaaga gctgccgcat gtacttctag
     5281 aagatcgggt tgtcgagcgg ctgcggcggc acgaggaaga acacgttgcg atcgtcgagc
     5341 tgcgtgactc cgtcgccgta gagtttgacc ttcgccttga acaactgcgg gacgtccttg
     5401 gccggcactg tcgtgttctg cttgctcacg accttccagc agacgggcgt gcccgtgcgg
     5461 acgtggaggt atttgtcgcg gaagccgtcg gcattcgtgt ccgtatcggt gaggccgttc
     5521 gcgcatgcgg cggtgccgag ctgcagcgtc tgcaggtgat cgacgaacga cgtgatcgcg
     5581 ttgacggagt cggtcggatc gtcgacgccg atcgcattga tgtcgagcgg caggccggat
     5641 gcgagcgtgc ggatgccgtt ttcgatcgcc gttgccgcgt tcgcgcccga gccatcgaac
     5701 acgagcggcg cgttgccgtt cgcggcgtcg accgcgcccg tgtccgcggc catcgtgcgc
     5761 atgtcggtct cgacggacgt gcccgcgacg ccggagccca tgatgccgac gagcttggcc
     5821 ttcgcgttca ggaatgattg cttcaccgtc gcgctccagc ccggcgacgt gtacgtgtca
     5881 cccgcggaga tcgccggctc gtcggtggcg agcaggatga cgggcagcgc gccttggcgg
     5941 aagcacgggt agccaaacgt tccgtagttg ttgagcgcgg ccggggagcc agcgcacgtg
     6001 ccacgcgcgg gcaccgagcc catcgtgaag tttgcgccac cttggccggt gaccgctgcg
     6061 tacgccgcga acgtgagcgg ctccatcgag tccgtgcccg tcggttcgct gacgggaatg
     6121 cccgagatgt tcgggttctt ctggatgtct ttgtagttct ggaacgccgc cgcgcccgag
     6181 ccgtgatagc cgatcgtgcc cgcgccggcc catagatcgg ggatgcacgt cgccgggtta
     6241 ctgccgagac cgccgcacgt gaggtgcgcg atggcggtcg cgaggttgtc gcgcacgctg
     6301 acgatttctt ggctcatcga gccggagcga tcgatcagca cgtacatgtc gacggcctgc
     6361 agctttgtcg agaagtcgag gtcgtcgtcg acgggcgact gcgtcttcat gtacggctcg
     6421 acgaacacga agtcgccgtt tgcctgcggg ttgctcgtcg cgttgttcgg gttcgtgccc
     6481 gccgcgatct cgatgaggtc cgacacgccg tcggcatcgg agtccgacgc cttcgagttg
     6541 gtctcgcctc catcgcgcac gccgttgcaa ttcgcgtctt cggtcacgtc cgcgacgcca
     6601 tcgtcatcgc tgtcgcgatc gatgaagtcg tagtgatggt ctgcgtctgt gtcgacgggc
     6661 ggcgagccac ccgcttcgat cttgtccggg acgcagtcgc cgtcggagtc ggtgtcgatg
     6721 aagttgccgc cgccatcacc gtcgtagtct tcgttcgtct cgaggacatc ggtgatcgtg
     6781 tcggcgtcgc tgtcgaggtc gtggaagtcg ggcgtgccgt ccgcgtcggt gtcgagcgcc
     6841 tgctgcgggt tgggcccgag ctcgccggtg tccggaatct gatcgccgtc gtcgtcgttg
     6901 tcttggaagt tcgcgaccga gtcgttgtcg agatcatcga gaccgtcgag cgtgtcggag
     6961 cgctcgttgt tgtcgctgtc gaggtcgcgg aagtccggcg tgccgtcgcc atcgctatcg
     7021 agcggcgcgg agtctgggtc gctatcgccg gcctcgcggt agtcggggat cccgtcgcca
     7081 tcgctgtcat cgtcttggaa gtcgggcgtg ccgtcgtggt cggtgtccga gagcgtcgcc
     7141 tggccttcgt cggcgtccga gatgaggtcg ccatcggcgt cgccgggcgt cccggtcccg
     7201 tcgtcgtcgc cccccgtaga cggaccgcag ctgacgagca cggcgagcag gcagacccgg
     7261 gcgagcttca tgggtgttgc gataccaagc gccatgccaa gaagacgtcg gcaggttcgc
     7321 ctctatgcat gcgaaatggc cgccttgacg tcgatgcagt gaggtctgct gacctccttc
     7381 ttttgtcctc agttacattc cgcggccatg gggtcgctcc tgctgatccg tcacggtcag
     7441 gctgcttacg gcgaggccga tcacgatcgg ctgacgccga ggggcgtcca gcaggcgcag
     7501 gcgctcggac attttctggc gaccgcgcgg atcgatcgcg tgtacgtcgg cccgcaccgc
     7561 cgtcaccagc agacgttcga tcacgcgcac gcgacgaagc cgctgccgga gccgacgctc
     7621 ttgcccggcc tcgccgagta tcccgccgtc gagctgttga cccacttcat gccgaagctg
     7681 atcgcggaag atccgcggtt cgccgagctg ccgaggacgc cgacgcgcga gctcgccggc
     7741 agagcgctgc acacgatcct cggcaggtgg gcccgcgacg agtggcaggt cgacggcgtc
     7801 gagcgcgtcg agaagttcgc cgagcgcgtg cgcggagcgc tcgatcaaat catcgccgaa
     7861 gccaagagcg gtgcgcgtat cgcggtgttc acgagcgcgg gcccgatcgg tgtctcggcc
     7921 gggctgacat tcggcgcgac gccgcatcac atggtgcgaa gcagcatcgt cattcgcaac
     7981 acgtcggtga gcgagatccg atttcgctcc gacgatttcg cctggcaccc cgaacgcatc
     8041 gcgctcgtct cgttcaactc gacgcatcac ctgccgcccg agctgcacac ggagtactga
     8101 catgaacttc gatccacccg agtccgtccg cccgctgctc gcgaagtacg agcaattcat
     8161 cagcgaggtc gtgatgccgg ccgaagcggt cgtgctcgag cgcggcttca aagaggccgc
     8221 accacagctg ctcgcgttgc gcgcgcgcgc gaagcaagaa ggcctctggc tcccgcagat
     8281 ggacaagcac gccggcggcc tcggcctgtc gctcgtcgag cacggcctcg ttagcgaacg
     8341 cctcggtcgc tcgccgctcg gtcactacgt gtgcaacgcg caagcgcctg acgccggcaa
     8401 catggagatc ctccacaagt acggcacgcc cgatcagaag aagcggtggc tcgagccact
     8461 gacgagcggc gagattcgct cgtgcttctc gatgacggag ccggagaatc ccggcagcaa
     8521 tccgacgctg ctctcgtgca ccgcaaagcg cgatggcgat cattacgtca tc
//
LOCUS       DATLBZ010000227        11157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6077766, whole genome shotgun sequence.
ACCESSION   DATLBZ010000227 DATLBZ010000000
VERSION     DATLBZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11157
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6077766"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2278)
                     /locus_tag="VMZ53_12005"
     CDS             complement(<1..2278)
                     /locus_tag="VMZ53_12005"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HUS29232.1"
                     /translation="MTTHSTLRQFLLGTALVTAGSCGSHSGPPAKDMAAMPPAQQQTA
                     FDKQIAKLVPAKTVKNAKLDATSSAIADYFQRNPSQRAYVMTDKPLYQPGETIWFRAD
                     LRATASLVEKSQMGMNAMLMSPRGAIVGQKRILAKDGVAANDFELSPDIEGGEYTMQL
                     TADDGTMTTKKIIVNTYEAPRLKKSVEFVRKAYGEGDTVTAAVEIKRATGEAFADQAL
                     TGIVTIDDVEVTRVQLKTSSDGTTTAKFTLPAKIAKGDGLLTILADDGGVTESIQKRI
                     PIVMKTMQVSLFPEGGDLVEDVPGRVYFTAKNTLGKPADIEGKVIDDRGTVVAQFSSV
                     HDGMGRFELTPSADRTYHVEITKPVGIAQKFDVPAAKAGGCVVRSVDQADAKQLRVAA
                     ICSSSRTVLVEAVVREKRIAAGAFEVTAGKPALIELPIDGEVQGAARVTLFSAKQEPL
                     AERLVYQGRGRDLKISMTADKKQYSPRDPVKLNIKTVDAEGKPVKANIGVAVVDDTVL
                     SYADDKSGRILAKLYLEPELNVTDADPIEEPNFYFGSKPEASAAMDALLATRGYRKFE
                     WQQVFAPPPPVTTATTGAGWGGEDKWAAVEGRAMELDEAPRAVAAAPPPMRAAEAKKP
                     RDADRKEQVAKGAGGGIAQAQNRPVLIKEADLAKDERQGRGRRIANAEKADPWAGDNE
                     DGLIAGGLSPVRVFPVPAYTKAYEGPRTDFRETIYWNANVSTNADGIASVSFPVSDAV
                     TSFRATAEGVSSGGLPGAG"
     gene            2392..3627
                     /locus_tag="VMZ53_12010"
     CDS             2392..3627
                     /locus_tag="VMZ53_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HUS29233.1"
                     /translation="MLVAAIAAMVVTLAIALGWRNPWAPAAGALLGVGVVIAAGVIGV
                     HDIELALRDLWRPLLVILSIMVTSSCAAELGVFSLLARWIEPRTRGPVRHAFRFTFIL
                     SAITAALLSNDAAILLVTPVVIELLRSVYPKRNPKFVVPFAFAAFVAAGVAPLPTGNP
                     MNMVVASRAGIHFNDYALHMIPVALAGWITAYIALAWCFRDALSDEAPALGQAPPAVV
                     LGTPARIVLVTIAVALASYPVLAVFDIRPWVMAVPAALVCLVAARGVGIGRISRGVSW
                     ELLPFVLGVLLLATALSRVGVTAELSELYARSPARLPTVGTVAAIGSALVNNHPMALL
                     HSVVLAGAPDRLVYAALVGGDLGPRLLPIGSLAGLLWLHALRTRGIDVPLTTFLRVGV
                     IVTIPSLIVSLAVLWLVTL"
     gene            3635..5749
                     /locus_tag="VMZ53_12015"
     CDS             3635..5749
                     /locus_tag="VMZ53_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29234.1"
                     /translation="MRWLLLLVAACGRIGFDATDTEPDAAVVGRWAKVSTDDSGTCAI
                     TLTGELWCWGAGGPGRLGRGAVHLQGIERVGTSTSWTSVSIGTSHSCGLQGDQLWCWG
                     TNNHGQLTGRAAFDVALPYQLPAAWSQIDVSEAHTCAIRSDGQLFCWGEGASNTLGNG
                     SQVDQSIPTAVAAGPSTWKQVATSEITTCAIGGDDSLWCWGFNSTGQVGDGTTMPRLT
                     PVQVDAARKWKSVDVAFFHTCAIDIDGVPYCWGDEYSGENNVGPSQARAPMPVMNMPV
                     ALESIDIGRYHSCGRTSDGSVYCWGSSEQGQLGILQQVVLPTKIATASAYNALREQTC
                     IIADARLSCAGRNGRGQVIPPAAELHEPTRADQRTDWQDIVVASRHGCGLVTGDATYC
                     WGENYRGELGNGTSSDIESPVPIGRTYARVAVGSGMAGGIDSAGAMWLGGVSPDQSQY
                     IPAHQQLYAPGFTSRVAFGAEHFCKVAPTNAVVCGGDNAHGQLGNGSFTNAAETAITG
                     SYIDISARENTTCAIDAGGMVACWGDNFYGQVGNGSTADANAPQLINTLTGVTQVALG
                     RTFTCALAGGEIWCWGAGSSGQLGPGSTGSTTPVKVSPRTDWARISAGDEHACAIDTS
                     GALWCWGHGDDGQLGVWSEGVYNSPALVRIGTETTWARVACGDHFTCALKTDGTRWCM
                     GSNNWAELGDGQGWHEQFTVIP"
     gene            complement(5751..6332)
                     /locus_tag="VMZ53_12020"
     CDS             complement(5751..6332)
                     /locus_tag="VMZ53_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29235.1"
                     /translation="MPSVRYQERSLAGEKQKLIIKPGHIATQTFDIDVFGHDDGSSRD
                     PNDSFRLIFQLGYGYFIYLFVVGMAQPDGLGRFTSFWLKDIVAVDIEMEEYGAPSCVE
                     GGPYELPVNWGGVTLWRGDQETARFSISGCAFEATTKEDDKLLLTMRWRLSDGGVLSW
                     TSATTNGWHLFSRQGDRELEAGEYVTFIDQRAT"
     gene            6416..7180
                     /locus_tag="VMZ53_12025"
     CDS             6416..7180
                     /locus_tag="VMZ53_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HUS29236.1"
                     /translation="MAFAQAASNRPIRGAVATLGVSDRIDFLRKTYAHLGGALIALAL
                     LTGLIIKYATNFSMQVATVSPWIVLIVFIGGNIFAQRLAMSESSRATQYAGLGLAVTI
                     WAFFLQGMIWFVIAKFGGAYVDPRTREIHYVMTGTAMNVILQSVVITLAIFIGLTVTV
                     FVTRKDFSFLRGVLSIALWAMIGVGIASWIFGFTFGALLSGVVVLIMAGYILYQTSMI
                     MNSFPPRMYVAAALMLFGTVATLFIHVLNIVASRDR"
     gene            7278..8252
                     /locus_tag="VMZ53_12030"
     CDS             7278..8252
                     /locus_tag="VMZ53_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUS29237.1"
                     /translation="MPAVVAPAPLSTTDIGAVRKLEDAIATVIRGKPEAIRAATVTLL
                     ARGHLLIEDIPGVGKTTLARAVAAALGGTFRRIQFTSDLLPSDIVGVSVYDSHGKVFE
                     LKRGPIFANVVLADEINRTTPRTQSALLEAMSEGQVSIDDATHDLPKPFMVVATQNPA
                     EHYGTYPLPESQMDRFLLRVSLGYPQKTIERDLLRNRHGGDPVAALQPVVDLSAVRTL
                     QDGVETIRMEDALLDYAMQIIEETRRHPAISVGISTRGALAWYRAAQAAALAAGREFV
                     IPDDIKALAIPCLSHRLALSQAHDSLGRQRTEAERVIAEIVGRVAVPT"
     gene            8249..9346
                     /locus_tag="VMZ53_12035"
     CDS             8249..9346
                     /locus_tag="VMZ53_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUS29238.1"
                     /translation="MTFWERLRRRLRPPRRLSFTREGKIIVVLSVGVGFAAINTGNNL
                     LYLLLGWLLSFIIASGVLSEMTLRALTVERRPPPRVFAGEPFLMEVVIKNGKPNRASY
                     SIEVEDLQGKTPIDKRCYFLKIPEAKSQRTSYRHTFVKRGLYTLSGYRLATKFPFALF
                     RKSRDVDAPLEMLVYPARVPVPRPPPRALTRGDAIADRLGRRGEFFGLRESRVGDDRR
                     DVHWKSTARTGRLLVREYEDELARRVVVAVDNALPKDVREAVADGHVTPAQEAQVSAV
                     ERGISIAASLAAAYLEVGWTVELVSRNCHVLPGNGRMHEAKIARALALLPYEGEETPF
                     SDMPKRIESVLVQPRGVPAIGRPSTQTVMDA"
     gene            9343..>11157
                     /locus_tag="VMZ53_12040"
     CDS             9343..>11157
                     /locus_tag="VMZ53_12040"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HUS29239.1"
                     /translation="MRFAVAHKAATYLMVGFAYLAMIAGGGIAPMIALAGVVGLIGSW
                     WWEPPNVKLERWAWIWTLASVFALIYSVLTAVVTGDFLGVGAQFLIWLVVAKAYNRRA
                     ARDWQQMYLLAFLMLVAGSVLNADLTYGVCFLGFVITATWAITLFHLRREMEDNLLVK
                     HAADRASERVEVRRILDSKRIVGTRFFVGTGFLSFGVFIIASIVFLAIPRVGFGFFLK
                     GHKGQTFSGFNDGVKLGGHGVIKNDSTVVMRVEIDAQYGGRDKPEVHWRGVAFDHYSK
                     GQWTRDPASPWNREIVEDGNPPSSVEKHKIIWPDAGPFLRDAAVKQGIWLDPLDSDVL
                     FGGGIPRQYEVAKIARKRKQLATRNQEMRLDHGETLHYTVWSDFFPPPPDELRAANGK
                     LSPFYENVYFQIPDEVRRDAELYKRVDEITAGLTNNYDKAKAIETWLETNLSYTLELA
                     DPGSVEPIHFFVFQRKKGHCEYFSSAFVIMARIAHIPTRQVNGFLGGEWNEYKGYVAV
                     RAGDAHSWAEVYFPGKGWVTFDPTPSAPGGELGRGGNGWTAKMGRMLDTLRFQWSKWV
                     IEYDLVSQISLFKDIGNAFKRAAVALRDVFKWLLSFWPL"
BASE COUNT         1793 a         3809 c         3483 g         2072 t
ORIGIN      
        1 cgcctgcacc gggcagaccg cccgacgaca caccctcggc cgtcgcgcgg aacgacgtca
       61 ctgcatcgga gaccgggaac gagacgctcg cgatgccgtc ggcattcgtc gagacatttg
      121 cgttccagta gatcgtctcg cggaagtcgg tacgcggtcc ttcgtacgcc ttcgtgtacg
      181 ccggcaccgg gaacacacgc acaggcgaga gcccgccggc aatgaggccg tcttcgttat
      241 cgcctgccca cgggtccgcc ttctcggcat ttgcgatccg ccgtccgcga ccctgccgtt
      301 cgtctttcgc gagatcggct tcctttatca ggacgggacg attctgggct tgcgcgatgc
      361 caccaccggc gcctttcgcg acctgctcct tgcgatcagc gtccctcggc ttcttcgctt
      421 cggcggcacg catcggcggt ggcgctgcgg cgacggcccg cggcgcttcg tcgagctcca
      481 tcgccctgcc ctctactgcc gcccatttgt cctcgccgcc ccaacctgcg cccgtcgtgg
      541 cggtggtcac cggcggcgga ggcgcgaaaa cctgctgcca ctcgaacttg cggtagccgc
      601 gcgtcgcgag cagcgcgtcc atcgccgcgg acgcttcggg cttgctgccg aagtagaagt
      661 tcggctcttc gatcggatcg gcgtcggtga cgttgagctc tggctcgaga tagagcttcg
      721 cgaggatgcg accactcttg tcgtctgcgt acgagagcac cgtgtcatcg acgacggcga
      781 caccgatatt cgccttcacc ggcttgccct cggcgtcgac ggtcttgatg ttgagcttca
      841 ccggatcgcg gggcgagtac tgcttcttgt cggcggtcat gctgatcttc agatcgcggc
      901 cgcggccttg atagacgagg cgctccgcga gcggctcttg cttcgccgag aagagcgtca
      961 cgcgcgcggc gccttggacc tcgccatcga tcggcagctc gatcagcgcc ggctttcccg
     1021 ccgtcacttc gaacgcgccg gccgcgattc gcttctcgcg gaccaccgct tcgacgagga
     1081 ccgtccgcga gctcgagcaa atcgctgcga cgcgcagctg cttcgcgtcg gcctgatcga
     1141 cgctgcgcac gacgcaacca ccggccttcg ccgcaggcac atcgaacttc tgcgcgatgc
     1201 caaccggctt cgtgatctcg acgtggtacg tgcgatcggc gctcggcgtg agctcgaacc
     1261 ggcccatgcc gtcgtgcacc gacgagaatt gcgcgacgac cgttccgcga tcgtcgatca
     1321 ccttgccctc gatgtcagcc ggcttgccga gcgtgttctt tgcggtgaag tacacgcgac
     1381 cgggcacgtc ctcgacgaga tcgccgccct cggggaacag cgacacctgc atcgtcttca
     1441 tcacgatcgg gatgcgcttc tggatcgatt cggtgactcc accgtcgtcg gcgaggatcg
     1501 tgagcaggcc atcgcccttc gcgatcttcg caggcaacgt gaacttcgcc gtcgtcgtgc
     1561 cgtccgagga cgtcttcagc tgcacgcgtg tgacctcgac gtcgtcgata gtgacaatgc
     1621 ccgtcaacgc ctgatcggca aacgcttcgc cggtcgcgcg cttgatctcg acagccgcgg
     1681 tgacggtgtc gccttcgccg tacgccttgc gcacgaattc gacgctcttc ttgaggcgcg
     1741 gcgcttcgta cgtgttgacg atgatcttct tcgtcgtcat cgtgccgtcg tcagcggtga
     1801 gctgcatcgt gtactcgccg ccctcgatat cgggcgacag ctcgaagtcg ttcgcggcga
     1861 cgccatcctt cgcgaggatg cgcttctgcc cgacgatcgc gccgcgcggc gacatcagca
     1921 tcgcgttcat gcccatctgg ctcttctcga cgagcgatgc cgtcgcacgc aggtcggcgc
     1981 ggaaccagat cgtctcgccg ggttgataca gcggcttgtc ggtcatcacg tacgcgcgct
     2041 gcgacggatt gcgctggaag tagtccgcga tcgcgctcga ggtcgcatcg agctttgcgt
     2101 tcttcacggt ttttgcggga accagctttg cgatctgctt gtcaaacgca gtttgctgct
     2161 gcgcgggcgg catcgctgcc atgtctttgg ccggcgggcc ggagtgcgag ccgcaactgc
     2221 cggcggtaac gagagcggtt ccgaggagga attgacggag cgtcgaatgg gtcgtcatga
     2281 cggttttcgt tccaacgggc cgtcttccga aatggtctag tcgcaccgca tccgcgcacc
     2341 agacacagcg ctcgtcgccg tccttgggtc cgtgcgtgtt accgtcgctg cgtgctagta
     2401 gcggccatcg ccgccatggt cgtgacactc gcgatcgcgc tgggttggcg caatccatgg
     2461 gcgcctgctg ccggtgcgct cttgggtgtg ggtgtggtca tcgccgcggg tgtgatcggc
     2521 gtccacgata tcgagctcgc gctgcgcgac ttgtggcgcc ctctcctcgt gatcctctcg
     2581 atcatggtga cgagctcgtg cgccgcggag ctcggcgtgt tctcgctgct cgcacgatgg
     2641 atcgaaccgc gcacgcgagg cccggtgcgt catgcgttcc gcttcacgtt catcctctcc
     2701 gcgattaccg ccgcgctgct ctcgaacgac gccgcgattc tcctcgtcac gcctgtcgtg
     2761 atcgagctgc tgcggagcgt ctatcccaag cgcaatccaa agttcgtcgt gccgttcgca
     2821 ttcgccgcgt tcgtcgccgc cggcgtcgcg ccgctgccga ccggcaatcc gatgaacatg
     2881 gtcgtcgcga gtcgcgcggg cattcacttc aacgactacg cgctccacat gatcccggtc
     2941 gcgctcgccg gatggatcac cgcgtacatc gcgctcgcgt ggtgctttcg cgatgcgctc
     3001 tccgacgaag cgcctgcact cgggcaagca cctcccgcgg tcgttctcgg cacaccggcg
     3061 cgcatcgtgc tcgtgacgat cgccgtcgcg ctcgcttcgt atccggtgct cgccgtcttc
     3121 gacatccgcc cgtgggtaat ggccgtcccc gctgcgctcg tctgtctcgt cgctgcgcgt
     3181 ggtgtcggca tcggacgcat ctcgcgcggc gtcagctggg agctcttgcc gttcgtgctc
     3241 ggcgtgctgc tgctcgcgac cgcactctcg cgcgtcggcg tcaccgctga gctctccgag
     3301 ctctacgctc gctcacccgc gcgcctgccc accgtcggca ccgtcgccgc tatcggctcc
     3361 gcgctcgtga acaatcaccc gatggcgctg ctccattcgg tcgtcctcgc cggcgcgccg
     3421 gaccgcctcg tctatgcagc gctcgtcggc ggtgacctcg gccctcgcct gcttccaatc
     3481 ggctcgctcg ccggtctgct gtggctacac gcgctgcgga cgcgcggcat cgatgtccct
     3541 ctgacgacgt tcctgcgcgt cggtgtgatc gtaacaattc cgtcactcat cgtgtcgctc
     3601 gcagttctct ggctcgtgac gttatgatcg ccggatgagg tggctgttgc tgctcgtcgc
     3661 cgcgtgcgga cgcatcggct tcgacgcgac ggacaccgag cccgatgcgg cggtcgtcgg
     3721 cagatgggcg aaggtctcga cggacgacag cggcacgtgc gcgatcacgc tgaccgggga
     3781 gctatggtgc tggggcgctg gggggcccgg acgtcttggc cgtggcgcgg tccacctgca
     3841 aggcatcgag cgcgtcggca cctcgacgag ctggacgtcg gtctccatcg gaacgtctca
     3901 cagctgcggg ctgcaaggcg atcagctctg gtgctggggc acgaacaatc atggccagct
     3961 gacgggtcgc gccgcgttcg acgtagcgct gccgtatcag ctgcctgccg cgtggagcca
     4021 gatcgacgtc tccgaagcgc acacgtgcgc gattcgcagt gatggccagc tgttttgctg
     4081 gggtgaaggc gcgagcaaca cgctcggcaa tggctcgcaa gtcgatcaat ccattcccac
     4141 cgccgtcgct gcgggtccga gcacatggaa acaagtggcg acgagcgaga tcacgacgtg
     4201 cgcgatcggt ggcgatgact cgctgtggtg ctggggcttc aacagcacgg gacaggtcgg
     4261 tgacggcacg accatgccgc gtctcacgcc ggtgcaagtc gatgcggcgc ggaagtggaa
     4321 gagcgtcgac gtcgcgttct tccacacgtg cgcgatcgac atcgacgggg ttccctactg
     4381 ctggggcgac gagtactccg gcgagaacaa tgttggacca tcgcaagcgc gtgctccgat
     4441 gccggtcatg aacatgcccg tcgcgctcga gtcgatcgac atcggccgct atcactcatg
     4501 cgggcgcacc agcgacggat cggtgtactg ctggggatcg agcgagcagg gccagctcgg
     4561 catactccag caggtggtcc ttccgacgaa gatcgcgacg gcctctgcgt acaacgcgct
     4621 gcgcgagcaa acatgcatca tcgcggatgc tcgattgagc tgcgcgggtc gcaacggtcg
     4681 tggccaggtc attcctcccg cggccgagct ccatgagccg acgcgcgccg accagcgcac
     4741 agactggcag gacatcgtgg tcgcgagccg tcatggttgc ggcctcgtga caggcgacgc
     4801 gacgtactgc tggggcgaga attatcgagg cgagctcggc aacggcacgt cgtccgatat
     4861 cgagagcccg gtaccgatcg gccgcacgta cgcgcgcgtt gcggttggca gcgggatggc
     4921 gggcggcatc gattccgctg gagcgatgtg gctcggcggc gtgagccccg accagtcgca
     4981 atacatcccg gcgcaccagc agctgtacgc gcccggcttc acgagccgcg ttgcattcgg
     5041 cgccgagcat ttctgcaagg tcgcgcccac caatgctgtc gtctgcggcg gtgacaacgc
     5101 gcatggtcaa ttgggcaacg gcagctttac gaatgccgcc gagaccgcca tcacaggctc
     5161 gtacatcgac atcagcgcgc gtgaaaacac gacgtgcgcg atcgatgctg gcggcatggt
     5221 cgcgtgctgg ggcgataact tttacggcca ggtcggcaac ggctcgacgg cggacgcgaa
     5281 tgctccccag ctgatcaaca cactgaccgg ggtgacgcag gtcgcgctgg gtcgcacgtt
     5341 cacgtgcgcg ctcgcgggcg gtgagatctg gtgttggggc gccggcagct cgggacagct
     5401 cggcccgggc tcgaccggta gcaccacacc cgtgaaagtc agcccgcgca cggactgggc
     5461 tcgcatctcc gcgggtgatg aacatgcctg tgcgatcgat acgtcaggcg cgctgtggtg
     5521 ctgggggcat ggggacgacg gccagctcgg cgtgtggagc gaaggcgtct ataactctcc
     5581 cgcgttggtt cgcatcggca ccgagaccac gtgggcgcgc gtcgcgtgcg gtgatcactt
     5641 cacatgcgcg ctgaagaccg acggaacacg ctggtgcatg ggctcgaaca actgggcgga
     5701 gctcggcgat ggccagggct ggcacgaaca attcaccgtg atcccgtagc tcacgtcgcg
     5761 cgctgatcga tgaacgtcac gtactcgccc gcctcgagct cgcggtcgcc ttgcctcgaa
     5821 aacagatgcc agccattcgt ggtcgcgctt gtccatgaca acacgccgcc gtcggagagc
     5881 cgccatcgca tggtgagcag cagcttgtcg tcctccttcg tcgttgcctc gaacgcgcat
     5941 ccggagatcg aaaagcgcgc cgtttcctgg tcgccgcgcc acagcgtgac gccgccccag
     6001 ttcacgggca gctcgtaggg gccgccttcc acgcagctcg gcgcgccgta ctcttccatc
     6061 tcgatgtcga cggccacgat gtccttcagc cagaagctcg tgaagcgccc gagcccatcc
     6121 ggctgcgcca ttccgacgac gaacaggtag atgaagtaac cgtagccgag ctggaagatc
     6181 aggcggaacg agtcattggg gtcgcggctc gagccgtcat cgtggccgaa aacgtcgata
     6241 tcgaacgttt gggtggcgat gtgccccggc ttgatgatga gcttctgctt ctcgccggcc
     6301 agcgatcgct cctggtaccg cacgctgggc atttcatcgt gatcccgtag gaataggtag
     6361 ggatccgagg gcggtttcac gtgctaagaa ccctatagat aaagaggcaa aacccatggc
     6421 cttcgcacaa gcagcatcca accgtccgat tcgcggagcc gtcgccactc tcggcgtcag
     6481 cgaccgaatc gattttcttc gtaagacata tgcgcatctc ggcggtgcgc tgatcgcgct
     6541 cgcgttgctc acgggcctga tcatcaagta cgcgacgaac ttcagtatgc aggtcgccac
     6601 ggtctcgccg tggatcgtcc tcatcgtgtt catcggcgga aacatcttcg cgcagcggct
     6661 cgcgatgtcg gagtcgtcgc gcgcaacgca gtacgcgggc cttggccttg ccgtcacgat
     6721 ctgggcgttc ttcctccagg gaatgatctg gttcgtgatc gcgaagttcg gcggcgcgta
     6781 cgtcgatccg cgcactcgcg agattcacta cgtgatgacg ggcaccgcga tgaacgtgat
     6841 cctgcagtcc gtcgtgatca cgctcgcgat cttcatcggc ctgaccgtga ccgtgttcgt
     6901 cacgcgcaag gacttctcgt tcttgcgtgg cgtcctgtcg atcgcgctgt gggcgatgat
     6961 cggcgtcggc atcgcctcgt ggatctttgg atttacgttc ggcgccctgc tgagcggcgt
     7021 cgtcgtgctg atcatggctg gctacatcct gtaccagacg tcgatgatca tgaattcgtt
     7081 cccgccacgg atgtatgtgg ccgccgcact catgctcttc ggcaccgttg cgacgctgtt
     7141 catccacgtg ttgaacatcg tggcgtcgcg cgaccgctag tttctgccga tttggcagcg
     7201 gcgcaggtac agagccgcga aatgacgggg gccgcagttc ggtccccgtc gtgcatttcg
     7261 gggctataat gggacgaatg cctgccgtgg tggcgccagc gccgctctcg acgaccgaca
     7321 tcggtgctgt ccgaaagctc gaagatgcaa tcgcgaccgt cattcgcggc aagcccgaag
     7381 caattcgcgc cgcgacggtc acgctgctcg cgcgcggtca tctcctgatc gaagacatcc
     7441 cgggcgttgg caagacgacg ctcgctcgcg cggtcgctgc cgcactcggc ggcacgtttc
     7501 gtcgcatcca attcacgagc gacttgttgc cgtcggacat cgtcggcgtc tccgtctacg
     7561 acagccacgg caaggtcttc gagctcaagc gcgggccgat cttcgcgaac gtcgtgctcg
     7621 ccgacgagat caaccgcacg acgccgcgca cgcagtccgc cctcctcgag gccatgagcg
     7681 aaggccaagt ctcgatcgac gacgcgacgc acgatctgcc gaagcccttc atggtcgtcg
     7741 cgacgcagaa ccctgccgag cactacggca cgtatccgct gcccgagtcg cagatggacc
     7801 ggttcctgct gcgcgtctcg ctcggctatc cgcagaagac gatcgagcgc gacctccttc
     7861 gcaaccgcca cggcggtgat ccggtcgccg cgctccagcc cgttgtcgat ctgtcagcgg
     7921 tccggacgct gcaagacggt gtcgagacga tccgcatgga agatgcgctg ctcgactacg
     7981 cgatgcagat catcgaggag acccgccgcc atccggcaat ctccgtcggt atctcgacgc
     8041 gtggcgcact cgcctggtac cgcgccgcgc aagccgccgc gctcgccgcc ggccgcgagt
     8101 tcgtcatccc cgacgacatc aaagctctcg cgatcccctg tctctcgcat cgcctcgcgc
     8161 tgtcgcaggc gcacgactcg ctcggtcgcc agcgcaccga agcagagcgc gtgattgcag
     8221 agatcgtcgg tcgcgtcgcc gtgccgacgt gacgttctgg gagcgccttc ggcgccggct
     8281 gcgtccgccg cgacgcctct cgttcacgcg agaaggcaag atcatcgtcg tcctctcggt
     8341 cggcgtcggc ttcgccgcga tcaacaccgg caacaatctg ctctacctcc tcctcggctg
     8401 gctgctgagc ttcatcatcg cgtcgggcgt gctctccgag atgacgctgc gcgcgctcac
     8461 cgtcgagcgg cggccaccac cgcgggtgtt cgcgggcgag cctttcttga tggaagtcgt
     8521 gatcaagaac ggcaagccga accgcgcgtc gtactcgatc gaggtcgagg acctgcaagg
     8581 caagacgccg atcgacaagc gctgctactt cctgaaaatc cccgaggcaa agtcgcagcg
     8641 aacgagctac cgccacacgt tcgtcaaacg cggcctctac acgctctctg gctaccggct
     8701 cgcgacgaag ttccccttcg cgctgttccg caagtcgcgc gacgtcgacg cgccgctcga
     8761 gatgctcgtc tatcccgcgc gcgtgcccgt gcctcgccca ccgccgcgcg cgctcacgcg
     8821 aggcgatgcg atcgccgatc gcctcggccg ccgtggcgaa ttcttcggtt tgcgcgaatc
     8881 gcgcgtcggt gatgaccggc gtgatgtgca ttggaagtcg accgcgcgca ccgggcgcct
     8941 cctcgtccgc gagtacgaag acgagctcgc acgccgggtc gtcgtcgccg tcgacaacgc
     9001 gctgcccaaa gacgtgcgtg aagccgtcgc cgacggccac gtcacgccgg cgcaagaagc
     9061 gcaggtctcc gccgtcgagc gcggcatcag catcgccgcc tccctcgcgg ccgcgtatct
     9121 cgaagtcggc tggaccgtcg agctcgtctc gcgcaactgc cacgtgctgc ccggcaacgg
     9181 ccgcatgcac gaagcgaaga tcgcgcgcgc tctcgcacta ctcccctacg aaggcgaaga
     9241 gacgccattc tcggacatgc cgaagcgcat cgagagcgtg ctcgtacagc cgcgcggtgt
     9301 gccggcaatc ggacgcccgt caacgcagac ggtgatggac gcatgagatt cgcggtcgcg
     9361 cataaagccg ccacctatct catggtcggc ttcgcctacc tcgcgatgat cgcgggaggc
     9421 ggtatcgcac cgatgatcgc gctcgctggc gtcgtcggcc tcatcggctc gtggtggtgg
     9481 gagccgccga acgtcaagct cgagcgctgg gcatggatct ggacgctcgc gtccgtgttc
     9541 gcgctcatct acagcgtgct caccgccgtc gtgaccggcg acttcctcgg cgtcggcgcg
     9601 caattcctga tctggctcgt cgtcgcgaaa gcctacaacc ggcgcgcagc tcgcgactgg
     9661 cagcagatgt atctgctcgc gttcctcatg ctcgtcgcag gctcggtcct caacgccgac
     9721 ctcacatacg gcgtctgctt cctcggcttc gtcatcactg ccacttgggc catcacgctc
     9781 ttccacctcc gccgcgagat ggaagacaac ctgctcgtca agcacgcggc cgaccgcgcg
     9841 agcgagcgcg tcgaagtgcg ccgcatcctc gacagcaaac gcatcgtcgg cacgcgcttc
     9901 ttcgtcggca ccggcttcct ctcgttcggc gtgttcatca tcgcgtcgat cgtctttctc
     9961 gcgatcccgc gcgtcggctt cggcttcttc ctcaaaggcc acaagggcca gacattctcg
    10021 ggcttcaacg acggcgtgaa gctcggcggc cacggcgtga tcaagaacga ctcgaccgtc
    10081 gtgatgcgcg tcgagatcga tgcccaatac ggcggccgcg acaaacccga agtccactgg
    10141 cgcggcgtcg cgttcgacca ctactccaaa ggccaatgga cacgcgaccc agcctcgccc
    10201 tggaatcgcg agatcgtcga agacggcaac ccgccgagca gcgtcgagaa acacaagatc
    10261 atctggcccg acgcaggccc gttcctccgc gacgccgcgg tcaagcaagg catctggctc
    10321 gacccgctcg actccgacgt cctcttcggc ggcggcatcc cacgccaata cgaggtcgcc
    10381 aagatcgcac gcaagcgcaa gcagctcgcc acacgcaacc aagagatgcg cctcgatcac
    10441 ggcgagacgc tccactacac cgtgtggtcc gacttcttcc caccaccacc cgacgagcta
    10501 cgcgccgcga acggcaagct ctcgccgttc tacgagaacg tgtacttcca aatcccagac
    10561 gaagtccgcc gcgacgccga gctctacaag cgcgtcgacg aaatcaccgc cggcctcacc
    10621 aacaactacg acaaagccaa agcgatcgag acgtggctcg agaccaacct ctcgtacacc
    10681 ctcgagctcg ccgaccccgg ctccgtcgag ccgatccact tcttcgtctt ccaacgcaag
    10741 aagggccact gcgagtactt ctcctccgcc ttcgtaatca tggcgcgcat cgcgcacatc
    10801 ccaacgcgcc aggtcaacgg cttcctcggc ggcgagtgga acgaatacaa aggctacgtc
    10861 gcggtccgcg cgggcgacgc ccactcgtgg gccgaggtct acttcccagg caaaggctgg
    10921 gtgacattcg atccaacgcc gtccgccccc ggcggcgagc tgggccgcgg cggcaacggc
    10981 tggacggcca agatgggccg catgctcgac accctccgct tccagtggtc gaagtgggtg
    11041 atcgagtacg acctcgtctc gcagatctcg ctcttcaaag acatcggcaa cgcgttcaag
    11101 cgtgcggccg tcgcactgcg cgatgtcttc aagtggctgc tgagcttctg gccgctc
//
LOCUS       DATLBZ010000228        27007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9806859, whole genome shotgun sequence.
ACCESSION   DATLBZ010000228 DATLBZ010000000
VERSION     DATLBZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 27007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27007
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9806859"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            767..1984
                     /locus_tag="VMZ53_12045"
     CDS             767..1984
                     /locus_tag="VMZ53_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29240.1"
                     /translation="MGYTERAAVERLRVAKAIEEVPQLGEAMTQGDLSFSGARELSRV
                     ITPETQQEWIDAAQEKSVRQIEEMVSGHKPGDRPTDGPDPALKTRVLRHEVRLSVAEL
                     ERRARKALQRQRGSAVDDSDFMEACFKALLDRVEGERAASRSKRSETSTVTDAVGTRT
                     IVGAEAVVVAPQDDMMELPTRGAAETSERGLVVGDRVLTESDIERLLVPRYQLAATRC
                     VDCKRGWQHGTSATSLLTPPELERAMCDCIDIGDVDADEPARAKRTVPKALKRHVLHR
                     DGYQCRVPGCRATENLDAHHIKFWMHGGVTTAPNLLCLCEGHHLAVHAGSLVITGDAV
                     TAAFTWSKQNSFKIETRAVECTAALRARGIAKRAVRTAVDATRTHVGQQDLTAQQWID
                     IALSKLPPEARGA"
     gene            2055..2843
                     /locus_tag="VMZ53_12050"
     CDS             2055..2843
                     /locus_tag="VMZ53_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS29241.1"
                     /translation="MRRWLMAATMCVAACGRISFDPLAATRDGAIAIDDSSGGGLDTT
                     ASIDAGLPAGLVGWYPFEAAATSDDVIANHDGMCGLNLCPQPAAGHIGGGVQFDGIDD
                     CFTVSDAGQLQLPQITLAIWANQATGGTLSHVSKRVVGGSVNSWQLETEALGMPANSL
                     SFTTYDNSGFNQYARTAANTIVLGTWQHLAATYDGTTKRVYINGIEQASVAEPTALPY
                     DNMPMMIGCDDNNPISEYYNGTLDDLQIYNRALSATEIADLAAR"
     gene            2858..4132
                     /locus_tag="VMZ53_12055"
     CDS             2858..4132
                     /locus_tag="VMZ53_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29242.1"
                     /translation="MKRAALLGLLVACAGKTQESGIAPLPPDLPDEHKAHATTKITLP
                     EQLAGVPLFVSDLDGFALFEEEQLQAANILATWARAQGLTVEDPKSTRQIFYRAARGQ
                     HAVTGQACGAPLWRTLAISRWREQMHAKGRIEAGVYCTPTCWLDVRISLGLDVGLDDG
                     PTAFYAAPFDPSQPWATELKKRLGELITWRAPGEPSEPIAAVPGAVPLPAVTDPTFHR
                     DQEQAQPAVPAALVAQADKCVGNAAGAGMVLEISASGVVEKCEPFDRRVIANVAVSDC
                     VCKAFVGQTIPGASGRMPLAVFGDETKVTTKAGLVVTASADAERALDPMTRLYKPVVS
                     DKSIEEWESPQPWVLAPCFATATGNVEASVKLEFGRDGHATNATFKATTGSLPKEAEA
                     CLKSVYMNIVAPCPDVEKSTATGVVHAMLAKP"
     gene            4194..4376
                     /locus_tag="VMZ53_12060"
                     /pseudo
     CDS             4194..4376
                     /locus_tag="VMZ53_12060"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4369..4629)
                     /locus_tag="VMZ53_12065"
     CDS             complement(4369..4629)
                     /locus_tag="VMZ53_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29243.1"
                     /translation="MPMEQRALEALEYKLKKRGFKQEDVYHHECPSCKEQAVRIYAIG
                     GKLGGRDIRVCLACSKTTSYRTGAGMEGRVEDQSFDLDDFLK"
     gene            complement(4646..5395)
                     /locus_tag="VMZ53_12070"
     CDS             complement(4646..5395)
                     /locus_tag="VMZ53_12070"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUS29244.1"
                     /translation="MDVKSQVSEAEWKLRVDLAAAYRLVALYGWDDLIFTHISARVPE
                     SDHHFLINPYGMTFDEVTASSLVKIDLEGRKVMDSPYEINPAGFTIHSAIHAAREDAQ
                     CVMHTHSVNGVAVSATKGGVLPVSQQSIFVLSSLAYHPYEGVALLEGEKPRLVRDLGD
                     KSFFMLKNHGLLTVGASIPDAFLFMYIFESACTIQLRAQSASPELITIDQKIVTGAQM
                     AAKQVTRNAGGALAWPGLLRKLDRLDRTYRD"
     gene            complement(5422..5793)
                     /locus_tag="VMZ53_12075"
     CDS             complement(5422..5793)
                     /locus_tag="VMZ53_12075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUS29245.1"
                     /translation="MATNYDIHEDVKFAPLEVIEAGALVDQCDEKWWNQTLCQVNESV
                     ARIGVFHGEFHWHKHDREDELFFVLEGTLLLDIEGKGSFELKPRQGMVVPHGVVHRTR
                     AAERTVVLMVEPAGVKPTGDS"
     gene            complement(5802..6056)
                     /locus_tag="VMZ53_12080"
     CDS             complement(5802..6056)
                     /locus_tag="VMZ53_12080"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HUS29246.1"
                     /translation="MDILTWIIVGLMAGVLASLLVGGYGLIADIVIGIVGAFVGGFIF
                     RAAGWASPFGGLAGTIFVAFIGAVVLLVILNLFNRRRAAV"
     gene            6139..6660
                     /locus_tag="VMZ53_12085"
     CDS             6139..6660
                     /locus_tag="VMZ53_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29247.1"
                     /translation="MHKAPTAAPQTRTPYLALFEKGKTWTIDKVTCRVAEVKPVGDST
                     VAHLACDPPNAGMLIVGTWVATPAGLYHPLVPIDEVDELTLLGEDDLLLTANAAEHSH
                     DRVIAGAKVSVEALVHEGSWCVRETTTAGSESRSYTLCFNSSGITGGSDSVVSAGETQ
                     ASTFGSVPADEVE"
     gene            complement(6662..7132)
                     /locus_tag="VMZ53_12090"
     CDS             complement(6662..7132)
                     /locus_tag="VMZ53_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HUS29248.1"
                     /translation="MRLLCILGILALCLLAPKADARTVTGYSHGQKTRIQLVTVSGVE
                     LEAATARAFKVMAKAARKAGIDIAIRSGFRSHEKQKQLYRDYKRGWGHLAAKPGYSNH
                     QNGKALDIYIDDYKVYEWLKKNATKFGFKKTVRREAWHWEYVGGGKAQARIAKR"
     gene            7334..8851
                     /locus_tag="VMZ53_12095"
     CDS             7334..8851
                     /locus_tag="VMZ53_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HUS29249.1"
                     /translation="MRVQIGCFLIASLAACTEETVAPPPEAPAIDAYVRSLPYLPVAQ
                     PQVTTGPSSAAQRDGDYSCTTENLKETRQFDRIVAYAANSDSLYPGAIVSADSVVSGL
                     FTQIVMPRAPETFSVSLENLSGTKKATIENPSLSTYREALSSVLASEITGSTPANIFS
                     EIEEVHTESQLNMALGVQAGWALGLASLKTSFDWANKSVRSRYVVRYTQAYYTVDLDA
                     PQSPSALLGTDVTLADVQAKMDAQHPPAYVSSVTYGRMIVFTFESQYSSSELGAALEF
                     AYSGGYDIKGDVSVTYKDILSSSKITAFILGGDAGSAAQTIDSYESLIAFIKGGGNYS
                     RQSPGAPIAYKLSYLQDNSPARMSFTTDYTTKDCVRVAQKIKVTLQSIAVESAAGDLD
                     NNLELYGTISASGTTLSTLFAKNSGSSVTIEEGKSYGSAAQPIAEGVIEVKPQAGQVI
                     RLRANLIDHDPAFGDDSSGDDTVLAPFETGWRKDVSVVLTGSGSRVRVNFQLAPI"
     gene            complement(8826..9878)
                     /gene="corA"
                     /locus_tag="VMZ53_12100"
     CDS             complement(8826..9878)
                     /gene="corA"
                     /locus_tag="VMZ53_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HUS29250.1"
                     /translation="MLGRIVDNGELKTITDCEEMKKAIAAGTHIWIELEAQCAEADDL
                     LVAALNIHPLTIEDIWAKRTQPKLEDYRNYLYLIVHALKGQKRQSLETVEVDVILGKT
                     FLITHDSGAEITKQVAADLQRDPSPLVKGPAWLAHLVLDEIVDQYLPVVDELDGAIES
                     LTNDALTRAGTPKGPPVLRRILRYKRLLQNMRRMSIHQREIFLRVGRGEFEEIPRETT
                     PFFRDVYDHFLRINDLVEGYRDLVTSALEAYLTVQANRMNEIMKTLTMISTVMLPLTF
                     IAGLYGMNFKHMPELEWVAGYPFALGLMAFIAAAILYWFRRKHWLGSDDIPIIDDDAP
                     KATKKRPGAKSAPTGS"
     gene            complement(9878..10690)
                     /locus_tag="VMZ53_12105"
     CDS             complement(9878..10690)
                     /locus_tag="VMZ53_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29251.1"
                     /translation="MRISAAVLCLVAGCSGCKDRDAVPKQAPSPRTTAVADENRPAPP
                     RPSLEPAEVSGLGVVPATQLGPKIVMSKDGISLDGQQVVAISEDGVIDRARLEALTQQ
                     LEQKATSDAPVALTLDATLPYRRIAILLDALKKGGFRNIALLTGSGSTMIPIELKDTN
                     EANTEGVRPIVTVKQNYVSVWSASGTEGTRKQPKLAYELTDPPDFKPVTRALADIVQS
                     HWPDGKRSDAERIIILQLDGDKPAQLLLQLAAAVRADGSLVLFPGIYLSAGA"
     gene            10715..10969
                     /locus_tag="VMZ53_12110"
     CDS             10715..10969
                     /locus_tag="VMZ53_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HUS29252.1"
                     /translation="MSDLAKQFADAQARIKPVTGLGNDTMLELYALYKQATTGDVAGD
                     RPGMLDLRGRAKYDAWAKKKGLSKDAAMTAYCALVDKHVR"
     gene            complement(10966..11871)
                     /locus_tag="VMZ53_12115"
     CDS             complement(10966..11871)
                     /locus_tag="VMZ53_12115"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HUS29253.1"
                     /translation="MNKLKVLATGICIAALAACSGEIVDDSSIVQDQDDPGVQEGSTS
                     VNRGCATFDLDDLQKARVEQDISNALAARGNVLAAGTTTAINVHVHIITDGAGKGAPT
                     SGMVSSQITVLNNAYATAGYSFVITSTDTTANSSWYTAQPNTTAESQMKNALRIGGAG
                     DLNMYFNNMGGGLLGWATFPSSYNSQPKMDGVVILTQSLPGGSASPYNHGDTATHEVG
                     HWMGLYHTFQGGCSGSGDGVSDTPAERSAAYGCPVSRDTCTGKRYPGLDPITNFMDYT
                     DDDCMDRFSPNQRTRMSNMWLTYRS"
     gene            complement(12018..14243)
                     /locus_tag="VMZ53_12120"
     CDS             complement(12018..14243)
                     /locus_tag="VMZ53_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29254.1"
                     /translation="MKRWLVLVLGVVACGDNDHSARVVMDVGEVAPAYGRSPFPTDAL
                     IEGRNIGPIARLDRMAKQHFDLIGAHLATLDGWGLRPTVQFFIEGELDADSVPIATRE
                     LSDALVVLDVDPNTGEDGAAIPFEWRYIRDRDTHRNYIAGSPAMGTQLREGTRYAAVL
                     TKDVKGIDGTPVFGARELGLLEEGPPARWQTTADAYTHLKTFSALEDRIAGIAVFTTQ
                     NASDTLVKARNEISNTSAVPAPTLTFDDDELIFDTPGKLTKLLGNAARETAGPRVGLE
                     RWGTDNANGMAHDHIAVIATGKTTIARFIGDDTGTDGPEDETFALGANGVPMVRSVDT
                     IPITVILPNTPMPATGFPVIVFGHGLGGSRRDMLNLAEPLCAKGYAMIGIDMWNHGLR
                     LSTTDTGNNLGSKPDFTGDRTIRDGFSDDADSVAYVSFFESFMNLSAIRDSIRQSALD
                     ISRVALLIQTGPSLLDLRGPFGATTPKLDKTRVAYLGQSFGTIVGTDLAAIEPTIGLY
                     VLNVAGGGLLDQIFPASAKIGELAVPLAEQLYRTQGTLDRFHPLVGAMQATFDGADSL
                     TFARHVLKDRFMIDNAYLDRRHVVVIEAIGDEIMPNRATEALARSFGLHLLRPNVQAP
                     TGMLQIESPGAGNVNNQTAILVQYEPATHGYNWSAERGELEYQPGYPFAGDVPYPKLP
                     AKITIKEPIYETLDQVSEILESYFAGENPRVRSTLAPVADFDGDGKPDTSDAYPFDPT
                     K"
     gene            complement(14261..15076)
                     /locus_tag="VMZ53_12125"
     CDS             complement(14261..15076)
                     /locus_tag="VMZ53_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29255.1"
                     /translation="MRPWLILVAAACSPSREQPPPPSHQPEPRHSIDVTVRDAPVAIR
                     GVELTPGPKHSLELGYRLEAQDKSVSIPGEIMCRVQGYNLVYPAATTTQVAGARLTGV
                     WRPDPFNEEPEICQVDWWLKRTRVAAACYAHGKLVDGACPGDTFTWDDNDGIHAIQLS
                     HAKLEFREGAAVMTGVFTVVDRVPEHHVARISCGDSGGPIVGEAAMPLLPLDRIGGGA
                     SVFGPVAILLDRSPRPLAACTFTIVAAGGKGEVALAEYCATTEAVRLGRCDGN"
     gene            15207..16757
                     /locus_tag="VMZ53_12130"
     CDS             15207..16757
                     /locus_tag="VMZ53_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HUS29256.1"
                     /translation="MRVRPRSPAFAGFAPFLGLSLLLTLAGACTAEVGVMPEPQQDSI
                     DDYIRSLPYLPVAPAQVVQGAASEPKRDGDYNCVTENLQETRQYDRIVAYAANSDSLY
                     PGALVSADSVLTGLFTQIVLPRQPETISVSLENLGGTKKATVKEPSLSAYREALAGIL
                     NAEITGSTPANLYSEIEQVHSQEQLNMALGIQASWGFGVASLKSSFNFSNEQIRSHYI
                     VKYTQAYYTVDLDAPSLPSELFPPEVTLDDVKQHMDDTRPPAYVSSITYGRMVLFTFE
                     SEYSAEEMGAALEFAYNGTADVSGQVSVTYKDIISKSKITAFILGGDAGTAVQTIDSY
                     EKLIAFIKSGGNYSKESPGAPIAYKLNYLKDNSPARMSTTTDYEMKTCERVSQKLRVV
                     LSAIAVDDAGGDSGDDLELYGAITASGVDSLVLFNKNSDNYVQIHQGNQFGQPIAEGI
                     LNVVPKAGQALTFRANLRDQDTFSPDDTIANDSVSAPFETGWRRDVVINGTGDSARIR
                     VVLSISPI"
     gene            16858..17730
                     /gene="panB"
                     /locus_tag="VMZ53_12135"
     CDS             16858..17730
                     /gene="panB"
                     /locus_tag="VMZ53_12135"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HUS29257.1"
                     /translation="MKTQRVTIHTLRQMKERGEKIAVLTAYDATFARLLDEGGADVLL
                     VGDSLGMVVQGNDTTLPVTLDEMAYHCRAVVRGTKRAHVVGDMPFMTYQSSVEQGMAN
                     AGKLIKEGGCHSVKLEGGAQHAELVSRLVGAGIPVMGHIGLTPQSYHQLGGFKVQGRE
                     QGGREKLLADARALEEAGVYALVLEAIPADIAREITASVSIPTIGIGAGAACDGQVLV
                     SYDMLGMDETFKPRFVRRFANLGNTIKEAVASYVSEVRGSTFPSDAESFAAAETKAPH
                     AQTGPLYSTVAGKK"
     gene            18086..18937
                     /locus_tag="VMZ53_12140"
     CDS             18086..18937
                     /locus_tag="VMZ53_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HUS29258.1"
                     /translation="MDLSALVELARGKRVVALTGAGCSTESGIPDYRGVDTPPLPRAP
                     IQHREFVDSEDNRRRYWARSLLGWPRLVQAKPNAGHHALAALERAGVIEGVITQNVDS
                     LHTEAGSRNVVELHGALARVRCLGCAAISSRASLQDRLLAANPTWAGLSRVIAPGAAE
                     LGPPKSRPDGDVDLSDEVVSSFVHVPCIECGGVLMPDVVFFGGSVPRDTLDSAWSLFD
                     RAELLLVIGSSLTVFSGYRFVRRAAEREVPVAILNRGPTRGDPHAQLRIDARIGDALP
                     LLVTALA"
     gene            18956..19447
                     /locus_tag="VMZ53_12145"
     CDS             18956..19447
                     /locus_tag="VMZ53_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29259.1"
                     /translation="MRKLVIIAIVVLASSTAVERSARADVGIGAFFGEPTGLDVKLGL
                     DRRSSLDIVVGWYSDWRHDGFNDGAYGHVTYLLTPMVGHGQSVNVPLRIGIGGAIYDQ
                     GGHFNDDLNLGVRFPFEVGLAFRRTPLEIYFEVALKVTVLDDNDNHPNVDLDGGIGIR
                     FYF"
     gene            complement(19438..19962)
                     /locus_tag="VMZ53_12150"
     CDS             complement(19438..19962)
                     /locus_tag="VMZ53_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29260.1"
                     /translation="MGKLDEAAFAKVIASCAKCEAKAFEVASYIDRQTTVMLGERNDD
                     GRWTHDGEKFVDGIYRVTCIRCRDDAFTTADCPRCHREGGLADALGHESRLAVPKRCP
                     TCKGTELTTTGFAPAKVRTGEGQRPSPTPTALFGDVGYHVANVMCEGCDWVATADGCP
                     LCGGSGALRARPQK"
     gene            20007..20264
                     /locus_tag="VMZ53_12155"
     CDS             20007..20264
                     /locus_tag="VMZ53_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29261.1"
                     /translation="MGINEACLAIEKFLLTYDGGSGKPVSVQVRPSGDDVDVIKVWVD
                     LGTGRRGMDTAAWERAAAASIREALPDTQAYRLQVRAETDG"
     gene            20335..22356
                     /locus_tag="VMZ53_12160"
     CDS             20335..22356
                     /locus_tag="VMZ53_12160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HUS29262.1"
                     /translation="MQPQQVHAALNRQQQTAVLHRGEAPLLVIAGAGTGKTTTLAHRI
                     AQLVIDGADPRRILLLTFSRRASQEMIRRAQRVIQHADIPWAGTFHAVGSRLLRMHAL
                     ALGLDPAFTVMDRGDAADLVDLVRVDRGLAKGERRFPKKDTCLAIYSRVVNARVPLEQ
                     ILNEAYPWCREHAAPLRQLFAAYVDEKLARHVLDYDDLLLWWWHAMSSPEVAALIRGR
                     FDHVLVDEYQDTNALQAEIIVGLSPSGKGVTAVGDDAQAIYSFRAASVHNILAFPTAF
                     TQRAEVVPLEDNYRSTVPILTASNAVIAESAQRYEKRLRSCRPSHEKPILALVDDELA
                     EVDYVIEKILAYREAGLQLRDQAVLVRAAHHADMLELELGRRRIPYVKYGGLKFLEAA
                     HVKDLLGILRWAENPIDPIAGFRTTQLVTGVGPAFARRAVDAIHSAPAGTPLLAPLTQ
                     LKPPAAAREQWPKLCDLLFALANPATPWLGQLGRVREFYEPILVERYDGAEARLGDLQ
                     QLESLSATYPSRAAFLDDLTLDPPSATGDLAGPPVIDEDWLILSTIHSAKGQEWKAVF
                     LLDVIDGRIPSDLSVGTQDQLEEERRLLYVAMTRAKDHLHLVQPLRMFVEKQHRFGDR
                     HVYAPRSRFLPDSILEHFDRVTRGRGVAGLVAEPAAVAVDVASRLRAMW"
     gene            22688..24289
                     /locus_tag="VMZ53_12165"
     CDS             22688..24289
                     /locus_tag="VMZ53_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29263.1"
                     /translation="MKLASISFLGLAAGLTVAAAGCIDLPGSGGTDDDLTSVGGDEYV
                     IDYDGYVDVAPGASDDVVKGVIHRQLKSALGALREQGIGVADRDAVRNLANPTLTRTR
                     VTVVAPGGAVRGELDRVRYHYHDQALVEKGHGTTSPLSLTLLYGDYIANAGSLIPICS
                     DDQTTAADSLWYHYQPNLSACKAAIVAEQRAIDHDREALSAATQIPASDADRKFLTTR
                     ATLVKATAAPDKWPEHDQLWGFAGDTSRTKLVVYSFFGVDSDINNPSDSGLVEYMRFE
                     RELRKRLPKLAVTETSPNAWLLDFYVDGQKLPNVTWDTVEKWIVDGTGFPASVANDQA
                     KKAALLRQVITNFSDRWIVWSAPMTIIQNGATRTMTLEIRTWHGFEDGSPDIRQRARW
                     RYLEAFWHADVFAYTGHSHFGHGPLEPWEYSAANFPDRYQVMLFNSCLSFNYYDEDFL
                     RMHPGGSQKLDVVVNGLPAYWMGMGGATAAYIAGITSGTQSWRQLLTGMRVNLPWQSS
                     YDPMRAVNGELDNQFTPSAGAISVQ"
     gene            complement(24290..24532)
                     /locus_tag="VMZ53_12170"
     CDS             complement(24290..24532)
                     /locus_tag="VMZ53_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29264.1"
                     /translation="MIDMLEESAMMRRPVVVSLSDGRVFEDRVKEIVKWEGEDHAVFK
                     DHELTPLSAIRTIQRGRPPELTYAGKHGTAGMQNGR"
     gene            complement(24606..25865)
                     /locus_tag="VMZ53_12175"
     CDS             complement(24606..25865)
                     /locus_tag="VMZ53_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29265.1"
                     /translation="MMNKLAASLFVFAVGCASATDGAFHVSGRVDDARVTHVVAGNPA
                     DGSRIIVAIEDGSFNLALPAGKQWVLTFADATKSGSAMQVATLQAGGLDALVPQEGGA
                     LDLGLVKMNGARASSTTDFGDIIDALGLSSAEATRMGNVDNLALRVANPDIDNDGFID
                     ERSPLLQVSGTFQVQLGGNSLSMADALRGDYKTASIKYMGTTLIAGVPSSMNMAMKTG
                     TLTFEQPFYGTAMGDSPAIMPGTPIGAPHVKFGQLDGMQTIGLVSRPGHDAPRGTYEL
                     GFANGALTFTDVMPPSTPIVESAQSYSVPFVHVIGTDSKCQGSCTIDSIALDWKSLSA
                     SGWTQATQQPAHVDIVLERGGKRSYLAADVSGTTMKWQDLDVTNTGLTWNELSYFTTA
                     NICYVGVSYESELGMKMTNALYNTGCF"
     gene            complement(26005..>27007)
                     /locus_tag="VMZ53_12180"
     CDS             complement(26005..>27007)
                     /locus_tag="VMZ53_12180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HUS29266.1"
                     /translation="NVGNEAIFAIDVTNGTAKPLVEKGSNNSAQLAGDRLVFSKDTLK
                     MPAELFTMKLDGSDVKQLTHFNDARVAKIQWGDYEQFSFKGAKGDTVYGYVMKPAGYT
                     SGKAPVAFLIHGGPQGSFGDHFHYRWNPEFYAGHGYATIFIDFHGSAGYGQAFMDEIS
                     GDWGGAPFEDLMSGLDAAIAKYPYLDGNRVAALGASYGGYMINWINGHTDRFKALVCH
                     DGIFDTKFGYYDTEEVWFPEWEHKGTPWTAPEEFEKFNPANFVKNWKTPTLVVHGSLD
                     YRIPESHGFAAFTALQRRKIPSRFLSLPDENHWVLKPQNSKVWHDEVLGWIDRYTKK"
BASE COUNT         4734 a         8784 c         8736 g         4753 t
ORIGIN      
        1 acacccacgt gggtcggcct gatcgcttcc gtccgcgagc tcgatgcgtc gcttgtcgtc
       61 ttacttcggc tcggctcaac ttcttcacga gatcgagttc gcggtgtcga tgcgacgcga
      121 tgaaccgacg tcttagggaa cgtcgcggca aattttcatt ccacatttct gcaggaagag
      181 atctccttcg tcgacgaatt catttgacgg cctacacgcg cagcgacaac acgagtcggc
      241 gacgcggcac gaaacagtgc gcgagacgcg accactcgcg ttctccgctg ccttcatcgc
      301 gcagtcgctt tctcgacgac aacgcacgat ttatcgcgct cacatcgcac ctcatcgctt
      361 cgaaaacctc ggtggtagaa cgagacgttg cgagtaaagc aggaggtgca gagtcatgtt
      421 gcgcgatgga tcgacaaccg agagcggcgc tggaacgacg aggggtgcgg aagtttggag
      481 cgcagtcgcg agcgagcgcg gcacgcgagc tacgagcgag cgcggcacgc gagctacgag
      541 cgaccacggc acgcgagcta cgagcgagca cgagcacgag cacgagcacg agcacgagca
      601 cgagcacgcg cagagctgcg agcgcgtgca ccgcacgttg aagcgaatcg tgaaagcgcg
      661 cggtgcgctg gacgcgcagg agtgtgcggc cttgcgcgag gcgcagacgt tgtgcgtttg
      721 gcgggcgtat gggtgcgcca gcttggtcga gtatatgacc cgcgagatgg gctacaccga
      781 gcgcgcggct gtcgagcggc tgcgcgtcgc gaaggcgatc gaggaggttc cgcagctggg
      841 cgaagcgatg actcagggcg acttgtcgtt ttcgggcgca cgtgagctca gccgcgtgat
      901 cacgcccgag acgcagcagg aatggatcga tgcggcgcag gaaaagagcg tccggcagat
      961 cgaagaaatg gtgtcggggc acaagcccgg cgatcggccg acggatgggc cggatcctgc
     1021 gttgaagacg agagtgctga ggcacgaagt gaggctgtcc gttgcggagc tcgagaggcg
     1081 tgcgcgcaag gcgttgcagc ggcagcgggg cagcgcagtg gacgacagcg acttcatgga
     1141 ggcgtgcttc aaggccctgc tagatcgcgt cgagggtgag cgcgctgcgt cgagatcgaa
     1201 acgcagcgaa acgagcaccg tcaccgacgc ggtcggcaca cgcacgatcg tcggtgcgga
     1261 ggcggtggtg gtcgcgccgc aggacgacat gatggagttg ccgacacggg gagctgcaga
     1321 gaccagcgag cgagggctcg tcgttggcga tcgcgtgctc accgagagtg atatcgaacg
     1381 tctgctcgtg ccgcggtatc agctcgccgc gacgcgatgc gtggactgca agcgcggttg
     1441 gcagcatggg actagcgcga cgagcttgct tacgccgccg gagctcgagc gtgcgatgtg
     1501 cgattgcatc gacattggcg acgtcgatgc agacgaaccg gcacgcgcga agcgcaccgt
     1561 gccgaaggcg ctcaagcggc acgtgcttca tcgagatgga taccagtgtc gtgtgccggg
     1621 ctgtcgtgcg acggagaatc tcgatgcgca tcacatcaag ttctggatgc acggtggcgt
     1681 gacgaccgcg ccgaacttgt tgtgcctgtg tgaaggccat cacctggcgg tgcacgcggg
     1741 ctctctcgtc atcacgggcg acgcggtgac ggctgcattt acgtggagca agcagaacag
     1801 cttcaagatc gagacgcggg ccgtggaatg tacggctgca ttgcgggcac gcggcatcgc
     1861 gaagcgggcc gtacggacgg cggtcgacgc aacccgaacc cacgtgggtc aacaggacct
     1921 gactgcgcag cagtggatcg acatcgcact gtcgaagctg ccaccggagg ctcgtggcgc
     1981 atgaagcacg agccgaagca cgagactcca ttaccgacga gcgagaggga cggcttgccg
     2041 cgtatcatta cgacatgagg cgatggctca tggcggcgac gatgtgcgtc gcggcgtgcg
     2101 ggcgcatcag cttcgatcca ctcgcggcga cgcgagatgg cgcgatcgcg atcgatgaca
     2161 gcagcggcgg cggtctcgac acgaccgcga gcatcgacgc tgggctacct gccgggctcg
     2221 tcggctggta tccgttcgag gcggccgcaa cctcagacga cgtgatcgca aaccacgacg
     2281 gcatgtgcgg gctgaacctg tgcccgcaac ccgcggccgg gcacatcggc ggcggtgtgc
     2341 agttcgacgg catcgatgac tgcttcaccg tcagcgatgc aggacagctg cagcttccgc
     2401 agatcacgct cgccatctgg gcgaatcagg cgacgggcgg cacgctgtcg cacgtgtcca
     2461 agcgcgtcgt gggcggaagc gtcaacagct ggcagctcga gacggaagcg ctcggcatgc
     2521 ctgccaactc gctctcgttc acgacgtacg acaacagcgg cttcaatcaa tacgcgcgca
     2581 ccgcggcaaa cacgatcgtg ctcggcacgt ggcaacacct cgcggccacg tacgacggca
     2641 cgacgaagcg cgtgtacatc aacggcatcg aacaagcgag cgtcgccgag ccgactgcgc
     2701 tgccctacga caacatgccg atgatgatcg gctgcgacga caacaacccc atcagcgagt
     2761 actacaacgg cacgctcgac gaccttcaga tctataatcg cgcactctct gcgacggaga
     2821 tcgcggacct cgcggcacgc tgacgtatga ttcgctcatg aagcgcgcgg cccttctcgg
     2881 tcttctcgtc gcctgcgcgg gcaagacgca ggagagcggc atcgcaccgc tgccaccgga
     2941 cctccccgac gagcacaagg cacacgccac gacgaagatc acactgcccg agcagctcgc
     3001 cggcgtgccg ctcttcgtga gcgacctcga cggcttcgcg ctcttcgaag aggagcagct
     3061 ccaagccgcg aacatcctcg cgacgtgggc ccgcgctcag ggcctcaccg tcgaggatcc
     3121 caaatcgacg cgccagatct tctatcgcgc cgcgcgcggc cagcatgcag tgaccgggca
     3181 agcgtgtggc gcaccgctat ggcggacgct cgcgatctcg cgctggcgcg agcagatgca
     3241 cgcgaagggg cgcatcgagg caggcgtgta ttgcacgccg acgtgctggc tcgacgtgcg
     3301 catctcgctc gggctcgacg tcggcctcga cgacgggccc accgcatttt acgccgcgcc
     3361 cttcgatccc tcgcagccgt gggcgacgga gctgaagaag cgcctcggcg agctgatcac
     3421 gtggcgagcg cctggcgagc cgagcgagcc gattgcagcg gtgcccggcg cggtgccgct
     3481 acccgccgtc accgacccga cgttccatcg cgatcaagag caggcgcagc cggccgtgcc
     3541 tgcggcgctc gtcgcgcaag cggacaagtg cgtcggcaac gcggccggcg cgggcatggt
     3601 gctagagatt tcagcgagcg gcgtcgtcga gaaatgcgag cccttcgatc gcagggtgat
     3661 cgcgaacgtc gcggtgtcgg attgcgtctg caaggcgttc gtcggtcaaa ccattcccgg
     3721 cgcgtccggc cgcatgccac tcgccgtctt tggcgacgaa acgaaagtga caacgaaggc
     3781 cgggctcgtc gtcaccgcaa gcgccgatgc cgagcgcgcg ctcgatccaa tgacgcgact
     3841 ctacaagccc gtcgtcagcg acaaatcgat cgaagagtgg gagtcgccgc agccttgggt
     3901 gctcgcgccg tgcttcgcga ccgcgaccgg caacgtcgaa gcgagcgtga agctggagtt
     3961 tggccgcgat ggtcacgcga cgaacgcgac gttcaaagcg acgaccggct ccctacccaa
     4021 agaggcagag gcctgcctga agagcgtcta catgaacatc gtcgcgccct gccccgacgt
     4081 cgagaagtcg acggcgaccg gcgtcgtcca cgcgatgctc gccaagccat gagccagtgc
     4141 tagctttggg catgctgtcg attcgctact gcagcgcttg agggtatcga gcgaaggctg
     4201 cccgggtggc ggccgagatc aagcgcgaga ccgggctcga tacgaagctc gtcgtcggcg
     4261 gctccggcga attcaccgtg tggctcgacg acaagttgct cgccgagaag aagatgggcg
     4321 tctttccaga tgccgccgcg gtcgttgcag cggtgaagag cgcgctcgct acttgagaaa
     4381 gtcgtcgagg tcgaagctct gatcttcgac gcgaccttcc atgccggcgc cggtgcgata
     4441 cgacgtcgtc ttgctgcacg cgaggcagac gcggatgtcg cgcccaccga gcttgccgcc
     4501 gatcgcgtag atgcgaacgg cctgctcttt acaggacgga cactcgtggt ggtagacgtc
     4561 ctcttgtttg aagcctcgct tcttcagctt gtactcgagg gcttcgaggg cgcgttgctc
     4621 catcggcatc gcgccgagta tcgcattaat cgcggtaggt gcggtcgaga cggtcgagct
     4681 tgcgaaggag gcccggccag gccagcgcgc cgccggcatt gcgcgtgacc tgcttcgctg
     4741 ccatctgtgc gccggtgacg atcttctgat cgatcgtgat gagctccggc gacgccgact
     4801 gcgcgcgcag ctggatcgtg caggcactct cgaagatgta catgaacagg aatgcgtctg
     4861 ggatcgacgc gccgaccgtg agtaggccgt gattcttgag catgaagaag ctcttgtcgc
     4921 cgaggtcgcg cacgagccgc ggcttctcgc cttcgagcag cgcgacgcct tcgtacgggt
     4981 gatacgcgag cgacgacagc acgaagatcg actgctgcga gaccggcagc acgccgccct
     5041 tcgtcgccga caccgcgacg ccgttgacgc tgtgcgtgtg catcacgcat tgcgcgtctt
     5101 cgcgagctgc gtggatcgcg ctgtggatcg tgaagcccgc gggattgatc tcgtacgggc
     5161 tgtccatcac cttgcggcct tcgagatcga tcttgacgag gctcgacgcg gtgacctcgt
     5221 cgaacgtcat gccgtacgga ttgatcagga agtgatgatc gctctcgggc acgcgcgccg
     5281 agatgtgcgt gaagatcagg tcgtcccagc cgtagagcgc gaccagccga tacgcagccg
     5341 cgaggtcaac gcgtagcttc cactcggctt cactgacctg cgatttaacg tccatgccgc
     5401 cgatgctaca tcagcgcggg atcaggaatc gccggttggc ttgacgcccg cgggctcgac
     5461 catgagcacc acggttcgct ccgccgcccg tgtccgatgc acgacgccat gcggcacgac
     5521 catcccttgc ctgggcttga gctcgaagct gcccttgccc tcgatgtcga gcagcagcgt
     5581 gccttcgagc acgaagaaca gctcgtcctc gcggtcgtgc ttgtgccagt ggaactcgcc
     5641 gtggaacacg ccgatgcgtg caaccgactc gttcacctgg cagagcgtct ggttccacca
     5701 tttctcgtcg cactgatcga cgagcgcgcc ggcctcgatt acctcgagcg gcgcgaactt
     5761 cacatcttca tgaatgtcgt agttggtcgc cacgagcgac gctatacagc ggcgcgtcgt
     5821 ctgttgaaca gattcagtat gaccagaagt acgacggcac cgatgaatgc gacgaagatg
     5881 gtgcccgcaa gtcctccaaa cggcgatgcc cagcccgccg cgcggaagat aaagccgccg
     5941 acgaacgcac cgacgatgcc gatgacgatg tcagcaatga ggccgtaccc gcccaccaga
     6001 aggctcgcga gcacaccggc catgagacca acaatgatcc aggtaagaat atccatgccc
     6061 ggggttagag catcacgcgt gccacatgct gcatcagtca ggactctcgt tctgctgtgc
     6121 gcgctctccg gctgtcgcgt gcacaaggcg cccaccgcgg caccgcagac gcgcactccg
     6181 tacctcgcgc tgttcgagaa gggcaaaacc tggacgatcg acaaggtcac gtgccgcgtt
     6241 gccgaggtaa agccggtcgg agattcgacg gtcgcgcatc ttgcctgtga tccgcccaac
     6301 gccgggatgc tgatcgtcgg cacgtgggtc gcgacgccgg ccggcctgta ccatccgctc
     6361 gtcccgatcg acgaagtgga cgagctgacg ttgctcggcg aagacgactt actcctcacg
     6421 gcgaatgccg cggagcactc gcacgatcgc gtgatcgcgg gtgcgaaggt ctccgtcgaa
     6481 gcgctcgtgc acgagggcag ctggtgcgtg cgtgaaacga cgacggccgg ctccgagagc
     6541 cgcagctaca cgctgtgctt caacagcagt ggcatcacgg gcggcagcga cagcgtcgtg
     6601 tccgcaggcg agacgcaggc ttcgacgttc ggctccgtgc ccgcagacga agtcgagtag
     6661 ctcagcgctt ggcgatgcgc gcttgcgcct tgccgccgcc gacgtactcc cagtgccacg
     6721 cctcgcgccg cacggtcttc ttgaagccga acttcgtcgc gttcttcttc agccactcgt
     6781 agaccttgta gtcatcgata tagatgtcga gcgccttgcc gttctgatga ttggagtagc
     6841 ccggcttcgc cgcgaggtgg ccccagccgc gcttgtagtc gcggtacagc tgcttctgtt
     6901 tctcgtgact gcgaaaccca gagcggatcg cgatgtcgat ccccgctttg cgtgccgcct
     6961 tggccatgac cttgaacgcg cgggcggttg ccgcttcgag ctcgacgccc gaaacagtga
     7021 cgagctggat gcgcgtcttc tgcccgtggc tgtagcccgt gaccgttcta gcgtctgctt
     7081 ttggcgcgag tagacacagc gccaagatcc ccagaatgca cagcaatctc atcggcgagt
     7141 cgtaatcatg aggccaccca agttgtcggg caactcttct agccgcgggg gccgctgtgc
     7201 acacaaaaat ttgcacttgc cgctgtctgt aacaaatcgg tcatcggcca ctacttggag
     7261 tgcaaacctt cgtgctcgct gcgatctgca catgttgcgc tggtctggcc gcttgcttgc
     7321 ttagtcgtcc ggcatgcgcg tccagatcgg ttgtttcctg atcgcttcac tcgcggcctg
     7381 cacagaggag acggtggctc caccgccaga ggctcccgcg atcgacgcgt acgtacgctc
     7441 gctcccctac ctgccggtcg cgcagccaca agtgacgacc ggtccatcga gcgctgcaca
     7501 gcgcgatggt gactactcgt gcacgaccga gaatctcaag gagacgcgcc aattcgatcg
     7561 gatcgtcgcg tacgccgcga actccgactc gctgtacccc ggcgcgatcg tcagcgcgga
     7621 ctcggtcgtc agcggcctgt tcacacagat cgtcatgccg cgtgcgcccg agacgttctc
     7681 ggtcagcctc gagaatctgt ccggcacgaa gaaggcgacg atcgaaaacc cgagcctgtc
     7741 gacgtaccgc gaggcgctct cgagcgtgct cgcttcggag atcaccggct cgacgcccgc
     7801 gaacatcttc tcggagatcg aagaggtcca caccgagtcg cagctcaaca tggcgctcgg
     7861 cgtccaagcg ggatgggcgc tcggcctcgc gagcctcaag acgagcttcg actgggccaa
     7921 caagagcgtc cgctcgcgct acgtcgtccg ctacacgcag gcgtactaca cggtcgacct
     7981 cgacgcgccg cagtcgccga gcgcgctgct cggtacggac gtcacgctcg ccgacgtgca
     8041 agcgaagatg gacgcgcagc atccgcccgc atacgtctcg tcggtcacgt acggtcgcat
     8101 gatcgtcttc acgttcgagt cgcaatacag ctcgagcgag ctcggcgcag cgctcgagtt
     8161 cgcgtactcg ggcggctacg acatcaaggg cgacgtctcg gtcacctata aagacatcct
     8221 ctcgtcgtcg aagatcaccg cgttcatcct cggtggcgac gcaggctcgg cggcacagac
     8281 gatcgacagc tacgaatcgc tcatcgcctt catcaagggc ggcggcaact actcgcgcca
     8341 atcgcccggc gcgccgatcg cgtacaagct cagctacctg caggacaact cgccggcgcg
     8401 catgtcgttc acgacggact acacgacgaa ggactgcgtg cgcgtcgctc agaagatcaa
     8461 agtgacgctg cagtcgatcg cggtcgagag cgccgccggt gatctcgaca acaacctcga
     8521 gctgtacggc acgatctcgg cctcgggcac gacgctgtcg acgctgttcg ccaagaacag
     8581 tggcagctcg gtcacgatcg aagaaggtaa gagctacggc agcgcggcgc agccgatcgc
     8641 cgaaggcgtc atcgaagtga agccgcaggc cggccaagtg atccggctgc gcgcgaacct
     8701 catcgaccac gacccggcat tcggcgacga ctcgtcgggt gacgacacgg tgctcgctcc
     8761 attcgagacg ggctggcgca aggatgtgtc ggtcgtgctg accggctcgg gctcgcgcgt
     8821 tcgcgtcaac ttccagttgg cgccgattta gcgcccggcc gcttcttcgt cgccttcggc
     8881 gcatcgtcgt cgatgatcgg gatgtcatcc gagccaagcc agtgcttgcg ccggaaccag
     8941 tacaggatgg cagcggcaat gaatgccatg agcccgagcg cgaatggata gcccgcgacc
     9001 cactcgagct ccggcatgtg cttgaagttc atcccgtaga gacccgcgat aaacgtcagc
     9061 ggcagcatca cggtcgagat catcgtcagc gtcttcatga tctcgttcat gcggttcgct
     9121 tgcacggtca gatacgcctc gagtgcgctc gtcacgagat cgcgataacc ctcgaccaga
     9181 tcgttgatgc gcaggaagtg atcgtagacg tcgcggaaga acggcgtggt ctcgcgagga
     9241 atctcttcga actcgccgcg cccgacgcgc agaaagatct cgcgctggtg aatgctcatg
     9301 cggcgcatgt tctgcagcag ccgcttgtag cgaaggatcc tgcgtaacac cggcgggccc
     9361 ttcggcgtac ccgcgcgcgt caacgcatcg ttcgtgaggc tctcgatcgc accgtcgagc
     9421 tcgtcgacga ccggcaggta ctggtcgacg atctcgtcga gcaccaagtg agcgagccac
     9481 gcggggccct tcacgagtgg gctcggatcg cgctgcaaat ccgcggcaac ctgcttcgtg
     9541 atctcggcgc cggaatcgtg cgtgatgaga aacgtcttgc cgaggatcac gtcgacctcg
     9601 acggtctcga gcgattgacg cttctgtccc ttcagcgcgt gcacgatcag atagaggtag
     9661 tttcgatagt cctcgagctt gggctgcgtg cgcttcgccc agatgtcttc gatcgtgagc
     9721 gggtggatgt tgagcgctgc gacgaggaga tcgtcggcct cagcgcactg cgcctcgagc
     9781 tcgatccaga tgtgcgtgcc agccgcgatc gccttcttca tctcctcgca gtcggtgatg
     9841 gtcttcagct cgccgttgtc gacaattcgt ccgagcatca ggcccccgcg ctcaagtaaa
     9901 tgcctgggaa cagcacgagc gagccatccg cgcgcacggc cgctgcgagc tgtaagagca
     9961 gctgcgcggg tttgtctccg tcgagctgca agatgatgat ccgctccgca tcgctacgct
    10021 ttccgtccgg ccagtgcgac tgcacgatat cggcgagcgc gcgcgtgacg ggcttgaagt
    10081 ctggcggatc ggtcagctcg taggcgagct ttggctgctt gcgcgtgccc tcggtgccgg
    10141 acgccgacca gacgctcacg tagttctgct tgactgtcac gatcggccgc acaccctcgg
    10201 tgttcgcctc gttcgtatct ttgagctcga tcgggatcat cgtcgagccg ctgccggtga
    10261 gcagcgcgat gttgcggaag ccgcccttct tgagcgcgtc gagcaggatc gcgatgcggc
    10321 gatacggcag cgtcgcgtcg agcgtgagcg cgaccggagc atcgctcgtc gccttctgct
    10381 cgagctgttg cgtcagcgcc tcgagccgcg cgcgatcgat gacgccgtcc tcgctgatcg
    10441 caacgacctg ctgcccgtcg agtgagatcc cgtccttgct catgacgatc ttcggaccga
    10501 gctgcgttgc gggcacgacg ccaaggcccg aaacctcggc gggttcgagc gaaggtcgcg
    10561 gcggcgccgg cctgttctcg tcagcgacgg ccgtcgtccg cggagacggc gcctgcttcg
    10621 gcacggcgtc gcggtccttg cagccgctac agccggctac caggcacagt acagccgccg
    10681 aaatcctcat gcgtgcggtg gtatcacaac gggcatgtcg gatctcgcca agcagttcgc
    10741 ggacgcgcag gcgcgcatca agcccgtcac cggtctcggc aacgacacga tgctcgagct
    10801 ctacgcgctc tacaagcagg caaccaccgg cgacgtggcc ggtgatcgcc caggcatgct
    10861 cgacctacgc ggtcgcgcga aatacgacgc gtgggcgaag aagaagggct tgtcgaagga
    10921 cgcggcgatg actgcgtact gcgcgctcgt cgacaagcac gtccgctagc tgcggtacgt
    10981 cagccacatg ttcgacatgc gcgtgcgctg gttcgggctg aagcgatcca tgcagtcgtc
    11041 gtccgtgtag tccatgaagt tcgtgatcgg atcgagaccc ggataccgct tgccggtgca
    11101 cgtgtcgcgg ctgaccgggc agccgtaagc cgcgctgcgc tcggcgggcg tatcggagac
    11161 gccgtcaccc gagccggagc agccaccttg gaacgtgtga tagaggccca tccagtgacc
    11221 gacttcgtgc gtcgcggtgt cgccgtggtt gtacggcgat gccgagccgc ccggcagcga
    11281 ctgcgtgagg atcacgacgc cgtccatctt gggctggctg ttgtagctcg acgggaacgt
    11341 cgcccaaccg agaaggccgc cgcccatgtt gttgaagtac atgttgaggt cacccgcgcc
    11401 gccgatgcgc agcgcgttct tcatctgcga ctcggcggtc gtgttcggtt gcgccgtgta
    11461 ccagctgctg ttcgcggtcg tgtccgtcga cgtgatgacg aacgagtagc ccgcggtcgc
    11521 gtacgcattg ttcagcacgg tgatctgcga cgagaccatg ccgctcgtcg gcgcgccctt
    11581 gcccgcacca tccgtgatga tgtggacgtg gacgttgatc gccgtcgtcg ttcccgctgc
    11641 caggacgttg ccgcgtgcgg cgagtgcgtt cgagatgtct tgctcgaccc gcgccttctg
    11701 caggtcatcg aggtcgaacg tggcgcagcc acggttcaca ctcgtcgagc cttcttgaac
    11761 accaggatcg tcttggtcct ggacgatgct cgaatcgtcg acgatttcac ccgagcacgc
    11821 cgccaacgcc gcgatacaga ttccggtagc gagaactttg agcttgttca tggtgggagc
    11881 ctccgacgtg ccggagcgtt ccacacacga accgccactg gcgatgtggg agttgtaggt
    11941 gcctcattga agtcagcaga tttgagacta cttggtgctc aaaccaacca cacttcacct
    12001 acatcgtcgc caacgcgcta cttcgttgga tcgaaaggat atgcgtcgga cgtgtccggt
    12061 ttgccgtctc cgtcgaagtc agcgacggga gcgagcgtgc tgcgcacacg cggattctcg
    12121 ccggcgaagt agctctcgag gatctcggaa acttgatcga gcgtttcgta gatcggctcc
    12181 ttgatcgtga tcttcgcggg cagcttcgga tacggaacat cgcctgcgaa cggatagccc
    12241 ggctgatact cgagctcgcc gcgctcggcg ctccagttgt agccatgcgt tgcgggctcg
    12301 tactgcacga ggatcgcggt ctggttgttc acgttgcccg caccgggcga ttcgatctgc
    12361 agcatgcccg tcggtgcttg cacgttcggt cgcagcaagt ggagcccgaa cgatcgcgcg
    12421 agcgcttcgg tcgcacggtt cggcatgatc tcgtcaccga tcgcttcgat cacgacgacg
    12481 tgccgtcggt cgaggtacgc gttgtcgatc atgaagcggt ctttgaggac gtgccgcgcg
    12541 aacgtgagcg agtccgcgcc atcgaacgtc gcttgcatcg caccgacgag cggatgaaac
    12601 cgatcgagcg tgccttgcgt gcggtagagc tgctccgcga gcggaaccgc gagctcgccg
    12661 atctttgcgc tcgccggaaa aatctgatcg agcagaccgc cgcccgcgac gttgagcacg
    12721 tagaggccga tggtcggctc gatcgccgcg aggtccgtgc cgacgatcgt gccgaacgat
    12781 tgcccgagat acgcaacgcg cgtcttgtcg agcttcggcg tggtcgcgcc gaacgggccg
    12841 cgcagatcga gcaggctcgg tcccgtttgg atgagcagcg cgacgcgcga gatatcgagc
    12901 gccgattggc ggatcgagtc gcggatcgcc gagaggttca tgaagctctc gaagaacgag
    12961 acgtacgcga cgctgtcggc atcgtcggag aagccatcgc ggatcgtgcg atcgccggtg
    13021 aagtccggct tactgccgag gttgttgccg gtgtctgtcg tcgacagacg caggccgtga
    13081 ttccacatgt cgatgccgat catcgcgtag cccttcgcgc agagcggctc ggcgaggttc
    13141 agcatgtcgc gacggcttcc gccgagaccg tgaccgaaca cgatcaccgg aaagccagtc
    13201 gcgggcatcg gcgtgttcgg gaggatcacg gtgatcggaa tcgtgtcgac ggagcgaacc
    13261 atcggcacgc cattggcgcc gagcgcgaac gtctcgtctt cggggccgtc ggtgccggtg
    13321 tcgtcgccga tgaagcgcgc gatcgtcgtc ttgccggttg cgatgaccgc gatgtgatcg
    13381 tgggccatgc cgttcgcgtt gtcggtgccc caccgctcga ggcccacgcg gggccccgcc
    13441 gtctcgcgcg ctgcgttgcc gagcagcttc gtcagcttgc cgggcgtatc gaagatcagc
    13501 tcgtcgtcgt cgaacgtgag cgtcggcgca ggaaccgcgg acgtgttcga gatctcgttg
    13561 cgcgctttga cgagcgtgtc gctcgcgttc tgcgtcgtga acaccgcgat gcctgcgatg
    13621 cggtcctcga gcgccgagaa cgtcttcaaa tgcgtgtatg catcggcggt cgtttgccag
    13681 cgcgccggcg gaccctcctc gaggaggccg agctcgcgtg caccgaacac gggcgtgccg
    13741 tcgatgccct tcacgtcctt cgtgagcacc gcggcgtagc gcgtgccttc gcgaagctgc
    13801 gtgcccatgg cagggctgcc cgcaatgtaa ttgcgatgcg tgtcgcgatc gcggatgtag
    13861 cgccactcga acgggatcgc ggcgccgtcc tcgccggtgt ttggatcgac gtcgagcacg
    13921 acgagcgcat ccgacagctc gcgtgtcgcg atcggcacgg agtcggcgtc gagctcgcct
    13981 tcgatgaaga actgcacggt cgggcgcaag ccccacccgt cgagcgtcgc caggtgcgcg
    14041 ccgatcagat cgaagtgctg cttcgccatt cggtcgagcc tcgcgatcgg accgatgttg
    14101 cggccttcga ttagcgcgtc ggtcggaaac ggtgaacgtc catacgcggg cgccacctct
    14161 ccgacgtcca tcaccacgcg tgcgctgtga tcgttatcgc cgcacgcgac aacgcccaag
    14221 accaagacaa gccaccgctt cacgaccgcg gagcatatat ctaattgccg tcgcagcgtc
    14281 cgaggcgaac cgcttctgtc gtcgcgcagt actcggccag cgcgacctcg cccttcccgc
    14341 ccgcagcgac gatcgtgaac gtgcacgcgg cgagcggccg tgggctgcga tcgaggagga
    14401 tcgcaacggg accgaacacc gatgcgccac cgccgatccg atcgagcggc aacagaggca
    14461 tcgcggcctc gcccacgatc gggccgcccg aatcgccgca cgatatccgc gcgacgtgat
    14521 gctcgggcac gcggtcgacg accgtgaaga cgccggtcat gacggccgcg ccctcgcgga
    14581 attcgagctt cgcgtgcgag agctgaatgg catggatgcc gtcgttgtca tcccacgtga
    14641 acgtgtcgcc ggggcacgcg ccatcgacga gcttgccgtg cgcgtagcag gcagcagcga
    14701 cgcgcgtacg cttcagccac cagtcgacct ggcagatctc tggctcctcg ttgaacggat
    14761 ccggacgcca cacacccgtg agccgcgcac ccgcaacctg cgtcgtcgtc gccgcgggat
    14821 agacgaggtt gtagccctgg acgcggcaca tgatttcgcc cgggatgctg accgacttgt
    14881 cctgcgcctc gagccggtag ccaagctcga gcgagtgctt cggtccaggc gtgagctcga
    14941 cgccgcgaat cgcgaccggt gcatcgcgca cggtgacgtc gatcgagtgc cgcggctcgg
    15001 gctggtgaga cggtggcggc ggttgttcgc gcgacggcga gcaggccgct gcgacgagga
    15061 tgagccacgg cctcacgaag tgaagatacg acagtggaag atcgactggc caggtcggct
    15121 gcagacatgg gtacgacggg cccgagacca agtacgcgaa cgctctgcat tctgcgctgg
    15181 ccacgtcctt gctttacgtc atgcgcatgc gcgtccgtcc ccgctcgcca gcgttcgctg
    15241 gcttcgctcc atttttgggc ctctcccttc tcctcacgct cgctggcgcc tgcaccgcgg
    15301 aggtcggcgt catgcccgag ccgcagcagg actcgatcga cgactacatc cgctcgctgc
    15361 cgtacctccc ggtggcgccc gcgcaggtcg tgcaaggtgc cgcgagtgag cccaagcgcg
    15421 acggcgacta caactgcgtg accgagaatc tccaggagac gcgccagtac gatcgcatcg
    15481 tcgcgtacgc ggcgaactcc gactcgctgt atcccggcgc gctcgtcagc gcggactcgg
    15541 tcctcaccgg cctgttcacg cagatcgtgt tgccgcgcca acccgaaacg atctcggtca
    15601 gcctcgagaa cctcggcggc acgaagaagg ccacggtcaa ggagccgagc ctctcggcgt
    15661 atcgcgaggc gcttgccgga atcctgaacg cggagatcac gggctcgacg cctgcgaacc
    15721 tctactcgga gatcgagcaa gttcactcgc aagagcagct caacatggcg ctcggcatcc
    15781 aggcgagctg gggcttcggc gttgcgagcc tcaagtcgag cttcaacttc tcgaacgagc
    15841 agatccgctc gcactacatc gtgaagtaca cgcaggccta ttacaccgtc gatctcgacg
    15901 cgccgagcct gccgagcgag ctgttcccgc ccgaggtcac gctcgacgac gtcaagcagc
    15961 acatggacga cacgcgcccg ccggcgtacg tctcgtcgat cacgtacggc cgcatggtcc
    16021 tgttcacgtt cgagtcggag tactcggccg aagagatggg cgctgcgctc gagtttgcgt
    16081 acaacggcac cgccgacgtc tcgggtcagg tgtcggtcac gtacaaggac atcatctcga
    16141 agtcgaagat caccgcgttc atcctcggcg gcgacgccgg cactgcggtt cagacgatcg
    16201 acagctacga gaagctgatc gcgttcatca agagcggcgg caactactcg aaggaatcgc
    16261 cgggcgcgcc gatcgcgtac aagctgaact acctcaagga caactcgccg gcgcgcatgt
    16321 cgacgacgac ggactacgag atgaagacgt gcgagcgcgt gtcgcagaag cttcgcgtgg
    16381 tgctctccgc aatcgcggtc gatgacgccg gtggcgactc gggcgacgac ctcgagctct
    16441 acggcgcgat cacggcgtcg ggcgtcgaca gcctcgtgct gttcaacaag aacagcgaca
    16501 actacgtgca gattcaccag ggcaaccagt tcggccagcc catcgcggaa ggcatcttga
    16561 acgttgttcc caaggccggc caggcgctga cgttccgcgc gaacctccga gatcaagaca
    16621 cgttttcccc cgacgatacg atcgcgaatg actcggtcag cgcgccgttc gagaccggct
    16681 ggcgccgcga cgtcgtcatc aacgggacgg gcgattccgc acgcattcgg gttgtcctct
    16741 cgatctcgcc catttgatgc tacggtccac gtaagtcctc gataggcgct cgtacccaga
    16801 tgttctgggg cgggacacca acaacccgaa ggacgaaaaa cagaaaggtc gggcggaatg
    16861 aagacccagc gggtcaccat ccacaccctc cggcagatga aagagcgagg cgagaagatc
    16921 gccgtactca ccgcgtacga cgcgacgttc gcgcggttgc tcgatgaagg cggagcggac
    16981 gttctcctcg taggcgattc cctcggcatg gtcgtgcaag gcaacgacac cacgctgccg
    17041 gtgacgctcg acgagatggc ctaccactgc cgcgcggtcg tccgcggaac gaagcgggcg
    17101 cacgtcgtcg gcgacatgcc gttcatgacg taccagtcgt ctgtcgaaca aggcatggcc
    17161 aacgccggca agctgatcaa agagggcggc tgccactcgg tgaagctcga aggtggcgcg
    17221 caacacgcgg agctcgtgag ccggctcgtc ggcgcgggta tcccggtgat gggccacatc
    17281 ggcctgacgc cgcagtcgta tcaccagctc ggcggcttca aagtccaagg gcgcgagcaa
    17341 ggcggtcgcg agaagctgct cgccgatgcg cgcgcgctcg aagaggctgg cgtctacgcg
    17401 ctggtgctcg aggcgattcc cgcggacatc gcgcgcgaga tcacggcgtc ggtgtcgatt
    17461 ccgacgatcg gtatcggcgc gggtgcagcg tgcgacggcc aggtgctcgt cagctacgac
    17521 atgctcggca tggacgagac gttcaagccg cgcttcgtgc ggcggtttgc gaacctcggc
    17581 aacacgatca aggaggcggt cgcttcctac gtgagcgagg tccgcggcag cacgttcccg
    17641 tcggacgcag agtcgttcgc agcggcggag acgaaagcac cgcacgcgca gaccggcccg
    17701 ctgtactcga cggtcgccgg caagaagtag gctggcgggc tacgccaccc cggccaggct
    17761 cgaatcgact cggatcgatc tcggatcgat tcaacaggga gacacggaga cacgaaggtc
    17821 ggaatacgga tattggcgcg ggtcgtgcgc caatccgcac cgtcgcgctc ggcccgaatc
    17881 ggcgcgccgc aatcggcccg ccttcggccc gctatctctc tgcttcgcgc gccgcaatcg
    17941 gcccgccttc ggccccctcg gccccctatc tctctgcttc gcgcgccgca atcggcccgc
    18001 cttcggcccc ccatctctct gcttcgtgtc ttcgtgtctc cctgttgaat catccggttc
    18061 tggtgacgcg tgctatccat gcggggtgga tctctccgcc ctcgtcgagc ttgctcgcgg
    18121 taagcgcgtc gtcgcgctga cgggcgcggg ctgctcgacg gagtcgggca ttcccgatta
    18181 tcgcggtgtc gatacacctc cgctgcctcg cgcgccgatc cagcatcgcg agttcgtcga
    18241 cagcgaggac aatcgccgcc gctattgggc gcgctcgctg ctcggctggc cgcggctcgt
    18301 gcaggcgaag ccgaacgctg ggcatcacgc gctcgcggcg ctcgaacgcg ctggcgtgat
    18361 cgaaggcgtc atcacgcaga acgtcgacag cctccacacc gaggccggta gtcgcaacgt
    18421 cgtcgagctg catggcgcgc tcgcgcgcgt gcgttgtctt ggctgcgccg cgatctcgtc
    18481 gcgtgcgagt ctgcaagaca ggctgctcgc ggcgaatccg acgtgggcgg ggctctcgcg
    18541 cgtgatcgcg ccaggcgctg ccgagctagg tccgccgaag tcgcgacctg atggcgatgt
    18601 cgatctgtcg gatgaggtcg tgtccagttt cgtacacgtt ccgtgtatcg agtgtggtgg
    18661 cgtactgatg cccgacgtcg tgtttttcgg cggaagcgtg ccacgcgaca cgctcgactc
    18721 ggcatggagc ttgttcgatc gcgcagagct gctgctcgtg atcggatctt cgctcacggt
    18781 gttcagcggt tatcgattcg tgcgacgcgc agcagagcgc gaggtgcctg tcgccatctt
    18841 gaatcgaggt ccgacacgcg gcgatccaca cgcacagttg cgcatcgatg cacgcatcgg
    18901 agacgcactg ccactacttg tcactgcgct cgcgtaacgc gttaggctcg tacgcatgcg
    18961 caagctcgtg atcatcgcga tcgttgtact cgcgtcatcg acagcagtgg agcgctccgc
    19021 gcgcgcggac gtcggcatcg gtgccttctt cggcgagccg acgggcctcg atgtaaagct
    19081 cggcctcgat cgccggtcct cgctcgacat cgtcgtcggc tggtacagcg actggcgcca
    19141 cgacggcttt aatgacggcg cgtacggcca cgtgacgtat ctcctgacgc cgatggtcgg
    19201 ccatgggcag agcgtcaacg tgccgcttcg cattggcatc ggcggcgcga tctacgatca
    19261 gggcggccac ttcaacgacg acttgaacct cggcgtgcgg tttccgttcg aggtcggcct
    19321 cgcgtttcgt cgcacgccgc tcgagatcta cttcgaggtc gcgctgaagg tcaccgtcct
    19381 cgacgacaac gacaaccacc cgaacgtcga tctcgacggc ggcatcggca tccgcttcta
    19441 cttctgaggt cgagcgcgaa gcgcgccgga gccgccgcac agcgggcagc catccgcggt
    19501 cgcgacccaa tcgcagcctt cgcacatgac gttcgcgacg tgatagccga catcgccgaa
    19561 caacgcggtc ggcgtcggcg acggacgctg gccttcgccg gtgcgcacct tcgcaggcgc
    19621 gaagccggtc gtcgtcagct cggtgccttt gcacgttggg caacgcttcg gcacggcgag
    19681 ccgggactcg tggccgagcg catcggcgag cccaccctcg cggtgacagc gcgggcagtc
    19741 cgccgtcgtg aaggcatcgt cgcggcagcg aatgcacgtg acgcggtaga ttccgtcgac
    19801 gaacttctcg ccgtcgtggg tccaccgacc gtcgtcgttg cgctcgccga gcatcaccgt
    19861 ggtctggcga tcgatgtacg acgcgacttc gaacgccttc gcctcgcact tggcacagct
    19921 cgcgatcacc ttcgcgaatg cggcttcgtc gagcttcccc atcgagcggg catcctacgc
    19981 gagcgcgtcg cgtgtaagct gggttcatgg ggatcaacga ggcgtgcctt gccatcgaga
    20041 agtttctcct cacctacgat ggcggctccg gaaagccggt gagcgtccaa gtgcggccat
    20101 ccggcgacga cgttgacgtg atcaaggtct gggtcgacct cggtacgggg agacgcggga
    20161 tggacacggc tgcgtgggag cgcgcggcgg cagcatcgat tcgcgaagcg ctgcccgaca
    20221 cgcaggccta ccgcctccaa gtccgcgccg agactgacgg ctgagcagat ctcgaccggg
    20281 gtaagtcaag atccggaagt tgcgcgggat cctacgtact gttcaaatgg acaggtgcag
    20341 ccgcagcagg tccacgccgc cctcaatcgc cagcaacaga ccgccgttct ccatcgcggc
    20401 gaggctccgc tgctcgtcat cgcgggggcc ggcacgggca agaccacgac gctcgcgcat
    20461 cgcatcgcgc agctcgtgat cgatggcgcg gatccgcgcc gcatcctgct gctgacgttc
    20521 tcgcggcgcg cgtcgcaaga gatgatccgg cgcgcgcagc gcgttatcca gcacgcggac
    20581 attccgtggg cgggcacgtt tcacgcggtc gggtctcgcc tgctgcgcat gcacgcgctc
    20641 gcgctcggcc tcgacccggc gttcacggtg atggatcgcg gcgatgcggc cgacctcgtg
    20701 gatctggtgc gcgtcgatcg cggcttagca aaaggcgagc ggcggtttcc caagaaggac
    20761 acgtgtctcg cgatctactc gcgcgtcgtc aatgctcgcg tgccgctcga gcagatcttg
    20821 aacgaagcgt atccatggtg tcgcgagcac gctgcgccgc tgcgccagct gttcgcggcg
    20881 tacgtcgacg agaagctcgc gcgtcacgtg ctcgattacg acgacttgct gctgtggtgg
    20941 tggcacgcga tgtcatcgcc ggaggtcgcg gcgctgattc gcggccgctt cgatcacgtg
    21001 ctcgtcgacg agtatcagga caccaacgcg ctgcaggccg agatcatcgt cgggctctcg
    21061 ccgagcggca aaggcgtgac cgcggtcggc gacgacgcgc aggcgatcta ctcgttccgc
    21121 gcggcgagcg tgcacaacat cctcgcgttc ccgacggcct tcacgcagcg cgccgaagtc
    21181 gtgccgctcg aggacaacta tcgctcgacg gttccgatcc tcaccgcgtc gaacgccgtg
    21241 atcgccgaat cagcgcagcg ctacgagaag cgattgcgca gctgccggcc ctcgcacgaa
    21301 aagccgatcc tcgctctcgt cgacgacgag ctcgcggaag tcgactacgt catcgagaag
    21361 atactcgcgt atcgcgaagc cggcctgcag ctgcgcgatc aagcggtgct cgtgcgcgcc
    21421 gcgcatcacg ccgacatgct cgagctcgag ctcggtcgcc gccgcattcc gtacgtcaaa
    21481 tacggcggcc tcaagttcct cgaagccgcg cacgtcaaag atctgctcgg cattctgcgc
    21541 tgggccgaga atccgatcga tccgatcgcg ggctttcgca cgacacagct cgtcaccggc
    21601 gtcggtcccg cgttcgcacg ccgcgccgtc gacgcgatcc attcggctcc cgcgggcaca
    21661 ccgctgctcg ccccgctgac gcagctcaag ccgcccgccg ccgcgcgcga gcagtggcca
    21721 aagctctgcg acctgctgtt cgcgctcgcg aatcccgcca cgccgtggct cggccagctc
    21781 ggccgcgtcc gcgaattcta cgagccgatc ctcgtcgagc ggtacgacgg cgccgaagcg
    21841 cggctcggcg atctgcagca gctcgaatcg ctctcggcca cctacccttc gcgcgccgca
    21901 ttcctcgacg acctcacgct cgatccaccg tccgcgaccg gcgacctcgc aggcccaccc
    21961 gtcatcgacg aagactggct catcctctcg acgattcact cggcgaaagg ccaggagtgg
    22021 aaagcggtct tcttgctcga cgtgatcgac ggccgcatcc cgagcgacct cagcgtcggc
    22081 acgcaggacc agctcgaaga agagcgccgc ctgctctacg tcgcgatgac gcgcgcgaag
    22141 gatcacctgc acctcgtgca gccgctgcgc atgttcgtcg agaagcagca ccgcttcggc
    22201 gatcgccatg tctacgcgcc gcgctcgcgc ttcttgcccg attcgatcct cgagcacttc
    22261 gatcgcgtca cgcgcggccg cggtgtcgcg gggctcgtcg ccgagcctgc cgcggtcgcg
    22321 gtcgacgtcg cgtcgcgcct gcgcgcgatg tggtgagtcc ctctccggaa gaaaaaacag
    22381 ggagacacga agcacacgaa gcagaggggg tcgtcgagga ggataaacag ggagacacga
    22441 agcacacgaa gcagaggctg atcggtcaac gcggttcccc cctccgcccc ctccgccata
    22501 tgtctctctg ccctccgtgt gctccgtgtc tccctgttca atcgtcttcg gggaagcggg
    22561 atgcccggct ggcaactggc gttgccactg acggcgttgc gaacatcgac gtgccgtcgg
    22621 ttgccagccg caaggccgca gaagcacagg gccggtccgt ggcctagagc gtgctcatgc
    22681 cgcggccgtg aaactggctt cgattagctt cttgggtttg gcggcgggac tgacggtggc
    22741 ggcggcgggt tgtatcgatc ttccgggcag cggcgggacg gatgacgacc tgacctccgt
    22801 gggcggcgac gagtacgtca tcgactacga cggctacgtc gacgtggcgc ccggcgcgag
    22861 cgatgacgtc gtcaagggcg tcattcatcg ccagctcaag agcgcgctcg gtgcgctgcg
    22921 cgagcaaggc atcggcgtcg ccgatcgcga tgcggtccgc aatctcgcga acccgacgct
    22981 gacgcggacg cgcgtgaccg tcgttgcgcc cggtggcgcg gtgcgcggcg agctcgatcg
    23041 agtgcgctac cactatcacg atcaagcgct cgtcgaaaaa ggccacggca cgacgagccc
    23101 gctctcgctc acgcttctgt acggcgacta catcgcgaac gccggctcgc tcattccgat
    23161 ctgcagcgac gaccagacga ccgccgcgga ctcgctctgg taccactacc agccgaatct
    23221 ctcggcgtgc aaagccgcga tcgtcgccga gcagcgcgcg atcgatcacg accgcgaagc
    23281 gctgagcgca gcgacgcaga ttcccgcgag cgacgccgac cgcaagttcc tgacgacgcg
    23341 cgcgacgctc gtgaaagcga ccgcagcgcc cgacaagtgg cccgagcacg atcagctgtg
    23401 gggattcgcc ggcgatacgt cgcgcaccaa gctggtcgtc tactcgttct tcggcgtcga
    23461 cagcgacatc aacaatccga gcgacagcgg cctcgtcgag tacatgcgct tcgaacgcga
    23521 gctccgcaag cggctgccga agctcgccgt caccgagacg agcccgaacg cgtggctcct
    23581 cgacttctac gtcgatgggc agaagctgcc gaacgtcacg tgggacaccg tcgagaaatg
    23641 gatcgtcgac ggcaccggct ttccggcgag cgtcgcgaat gatcaggcga agaaggccgc
    23701 gctgctgcgc caggtgatca ccaacttctc cgatcgctgg atcgtgtgga gcgcgccgat
    23761 gaccatcatc cagaacggcg cgacgcgcac gatgacgctg gagattcgca cgtggcacgg
    23821 cttcgaagac ggctcgcccg atatccggca gcgcgcgcgg tggcgctacc tcgaagcgtt
    23881 ctggcacgcg gacgtgttcg cgtacaccgg ccacagccac ttcggtcacg gcccgctcga
    23941 gccgtgggag tactcggcgg cgaacttccc ggatcgctac caagtcatgc tcttcaactc
    24001 gtgcctgtcg ttcaactact acgacgagga cttcctgcgc atgcaccccg gcggctcgca
    24061 gaagctcgac gtcgtcgtca acgggctccc cgcgtattgg atgggcatgg gcggcgcaac
    24121 ggctgcgtac atcgccggca ttacgagcgg cacgcagtca tggcggcagc tgctcaccgg
    24181 gatgcgtgtg aatctgccgt ggcagagcag ctacgacccg atgcgcgcgg tcaatggcga
    24241 gctggacaac cagttcacgc catcggccgg cgcgatctcg gtccagtagt cagcgtccgt
    24301 tctgcattcc agccgtgccg tgcttgcctg cgtaggtcag ctccggcggc ctgccccgct
    24361 ggatggtcct gatcgccgac agcggcgtca gctcgtggtc tttgaagacc gcgtggtctt
    24421 cgccttccca cttcacgatc tccttcacac gatcctcgaa cacccgtcca tcgctcagcg
    24481 aaacgacaac aggtcggcgc atcatggccg actcttcgag catatcgatc acgtcaccga
    24541 tatcgtatcg atgtcccatt gggtgcctcc tatcagaagc ctccctcccg ccgggacatc
    24601 ggcaatcaga aacatcccgt gttgtagagc gcgttcgtca tcttcattcc gagctccgat
    24661 tcgtagctga cgcccacgta gcagatgttt gccgtcgtga agtagctgag ctcgttccag
    24721 gtgaggccgg tgttcgtcac atcgaggtct tgccacttca tcgtggtgcc gctgacgtct
    24781 gctgcgagat acgagcgctt gccaccacgc tcgagcacga tgtcgacgtg cgcgggctgc
    24841 tgcgtcgcct gcgtccaacc cgacgccgac aggctcttcc agtcgagcgc gatcgagtcg
    24901 atcgtgcacg agccctggca cttgctgtcg gtgccaatga catggacgaa cggaaccgag
    24961 tagctctgag cgctctcgac gatcggcgtg ctcggcggca tcacatccgt gaacgtcagc
    25021 gcgccattcg cgaagccgag ctcgtacgtg ccgcgcggcg cgtcgtgacc ggggcgcgag
    25081 acgagaccga tggtctgcat gccgtcgagc tgaccgaact tgacgtgcgg cgcaccgatc
    25141 ggggtgccgg gcatgatcgc gggcgagtcg cccatcgccg tgccgtagaa cggctgctcg
    25201 aacgtgagcg tgccggtctt catcgccatg ttcatcgagc tcggaacgcc cgcgatgagc
    25261 gtggtgccca tgtacttgat gctcgcggtc ttgtagtcac cgcggagggc gtccgccatc
    25321 gacagcgagt tgccgccgag ctggacttgg aacgtgccgc tgacttggag gagcggcgag
    25381 cgctcgtcga tgaagccgtc gttgtcgatg tcggggttcg cgacgcgcag cgcgaggttg
    25441 tcgacgttgc ccatgcgcgt cgcttcggcg ctgctcaggc cgagcgcgtc gatgatgtcg
    25501 ccgaagtccg tggtcgagct cgcgcgcgcg ccgttcatct tgacgaggcc gaggtcgagc
    25561 gcgccgcctt cttgaggaac gagcgcatcg agaccgcccg cttgcagcgt cgcgacctgc
    25621 atcgccgagc ccgacttcgt tgcatcggcg aacgtcagca cccactgctt gcctgccggc
    25681 agcgcgaggt tgaacgagcc gtcttcgatc gcgacgatga tgcgcgagcc gtctgccggg
    25741 ttaccggcaa cgacgtgcgt cacgcgtgcg tcatcgacgc gaccggagac gtgaaacgcg
    25801 ccgtccgtcg cggacgcgca gccaacagcg aacacgaaga gggatgcggc gagtttgttc
    25861 atcatggtga cgacggctat tgcggctgcc gcgccaactc gcccgcattc tcaagtacgc
    25921 gggaccacac tctttgatct ggggtaattt ttgccccagg gtccgccgct accctcggta
    25981 atcctacccg gtgccgatgt acgcctactt cttcgtatag cgatcgatcc agccgagcac
    26041 ttcgtcgtgc cacaccttcg agttctgcgg cttgagcacc cagtggttct catcgggcag
    26101 cgagaggaag cggctcggaa tcttgcgacg ctgtagcgcc gtgaacgcgg cgaagccgtg
    26161 ggactcgggg atgcggtagt cgaggctgcc gtgcacgacg agcgtcggcg tcttccagtt
    26221 cttcacgaag ttggcagggt tgaacttctc gaactcttcg ggcgcggtcc acggcgtgcc
    26281 cttgtgctcc cactccggga accagacttc ctccgtgtcg tagtaaccga acttcgtgtc
    26341 gaagatgccg tcgtgacaga cgagcgcttt gaagcgatcc gtgtggccgt tgatccagtt
    26401 gatcatgtag ccgccgtacg acgcgccgag cgcagcgacg cggttgccgt cgaggtaggg
    26461 atactttgcg atcgcagcgt cgaggccgct cattaggtct tcgaatggcg cgccgcccca
    26521 gtcgccgctg atctcgtcca tgaacgcttg gccgtagcca gccgatccgt ggaagtcgat
    26581 gaagatcgtg gcgtagccgt ggcccgcgta gaactctggg ttccagcggt agtggaagtg
    26641 atcgccgaag ctgccctgcg gtccgccatg aatgaggaac gcgaccggcg ccttgccgct
    26701 cgtgtagccg gcgggcttca tcacgtagcc gtagaccgtg tcgcccttcg cgcccttgaa
    26761 gctgaactgc tcgtagtcgc cccactggat cttcgcaacg cgcgcgtcgt tgaagtgagt
    26821 gagctgcttc acgtcgctac cgtcgagctt catcgtgaag agctccgccg gcatcttgag
    26881 cgtgtccttg gagaagacca ggcgatcgcc ggcgagctgc gcggagttat tcgagccttt
    26941 ctcgacgagc ggcttcgcgg ttccgttcgt cacgtcgatt gcaaagatcg cctcgttgcc
    27001 gacgttc
//
LOCUS       DATLBZ010000229        11332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5451945, whole genome shotgun sequence.
ACCESSION   DATLBZ010000229 DATLBZ010000000
VERSION     DATLBZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11332
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5451945"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(341..1441)
                     /locus_tag="VMZ53_12185"
     CDS             complement(341..1441)
                     /locus_tag="VMZ53_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HUS29267.1"
                     /translation="MLRTFASLAALSLPLLATAHTAAADQCTPARVLVVLDKSSSMVT
                     GQINGVTKWDTAADGLGKLLEQYQTKAEFGLMTFPQPNQCGPGALDVAPALNNKASII
                     SALGSPPPTSGNYTPMSQSLEAAANLSAMSVTGPKHVVLITDGWQYCVPYDNGTRFDG
                     VDAIAALNAKGVTTWIVGFGGEVDALGLNKMAVAANTAKTGCNPTSEDAAAANNCYFQ
                     VDNAQELLTALNQIAGSVSSETCDGLDNDCDGKVDEDLTRACSNACGAGTETCHAGNW
                     EGCTAPPASASTEVCDGVDNDCDGQTDEPGADLCDNGDVCDDGMCVPPNGEDDGGGMH
                     AGCCDAGGAPTGQSLALFGLVGLALFARRRRR"
     gene            complement(1607..3391)
                     /locus_tag="VMZ53_12190"
     CDS             complement(1607..3391)
                     /locus_tag="VMZ53_12190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29268.1"
                     /translation="MSSDKADQPTIKVAITGDELAGEDRPEPAPNAIVSGRFRLGRRI
                     GKGGMGEVMAARDEQIARDVAVKRMRAANPSEKSIQRFLREAMVQGRLEHPAIVPVHE
                     IGRDSDGLPYFVMKKLTGTSLAKILEDRAHMPLQRVLRAFADVCLAVEFAHVRGIVHR
                     DLKPDNIMLGDYGEVYVLDWGVAKIIGEEDGEFADIGSGSGEHVTRAGTAIGTPGYMA
                     PEQVRGLTDIDGRADVYTLGCLLFEILSGEPLHPRGQLGMESALTDIDARPSVRAPDR
                     EIAPELDEMCVKATAVARANRTQTARELGERVERFLDGDRDLALRRNLAREHLDRARA
                     AFANDSEENRRTAMREAAGALALDPALSGAAELVGRLMLEPPRETPIEVKEAMTAVDV
                     HDAKAIAKAGVWAVIGSLLFLPLVWWMAPGSVNGMWVLAVLLAIDGVVAVVAVRAVKP
                     RPGLVVIANTVIVIAVARMFSPILIAPGVAASLGMAMVLTPRFSWLGSPITIGALMVG
                     AMLVPLGLEQLGIVSTTMTVTPHGVVFSGPALDGARSTPTIVVAALYAACLAGASIFA
                     GYQMRTRALTAAHSLQLQAWQLRQLVPR"
     gene            3510..4670
                     /locus_tag="VMZ53_12195"
     CDS             3510..4670
                     /locus_tag="VMZ53_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29269.1"
                     /translation="MKRFSLLALSFTACIDSSSTDLDLASADPEITSAEKADAPSWEN
                     AATLHEGTRIFDRAGANSRRVHSLWISGSNMNHVPLVVDARATDANDVRIAVLGPLVN
                     GVRPVLAADGYAQRKRDASVTLDISKPGEHLVVVGSYNLASETFYDLHATCPGPDGCG
                     VSRYDVLATPKDGALVGDSTRLVSMLLGDVMVGHSYDVNVELWASPPMQPWNATKVGT
                     STASGTQVNAIIPASVHEGDDLKLVVRQAGGRVLDTGVTTRFFPNPHSFARLDSILYG
                     DIASLQIAGVVGYFEGVADMRLFSETREMELAHTTLHADRPGQEGNGYNSFDATFLPD
                     YATNASLAPHDGEILSVGYINGNGDYRRLGCFEYCNNLSGLSSCTGGARSCP"
     gene            4687..5079
                     /locus_tag="VMZ53_12200"
     CDS             4687..5079
                     /locus_tag="VMZ53_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HUS29270.1"
                     /translation="MEQDPFTLVGGADKIRALVETFYDVMSEREPALARLHKCDPDGK
                     VDRGSRDRFALFLIGWLGGPDDYVEQHGHPRLRMRHGRVAVNLAMRDAWLRCMQVAMD
                     QHAISGDVRTFLDARFAEVADFMRNVDG"
     gene            complement(5295..5633)
                     /locus_tag="VMZ53_12205"
     CDS             complement(5295..5633)
                     /locus_tag="VMZ53_12205"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUS29271.1"
                     /translation="MLDYEKLDVYQCSIEHLTFVFKVVPKIPRGHSAVSDQWKRAAMS
                     IPLNIAEAVGKKTAADRENRYAIARGEAMECGAILDVIRFLRIASDSDIADAKRCITR
                     IVEMLSKMCR"
     gene            complement(5868..7175)
                     /locus_tag="VMZ53_12210"
     CDS             complement(5868..7175)
                     /locus_tag="VMZ53_12210"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HUS29272.1"
                     /translation="MSRKGDHIKRVGLVFSGGPAPAANAVISSAAISFLEDNRECIGF
                     FHGYSNLEGYHPVTHRLLPDEHYRIFNLKDLSGLRNTRGIVIGTARTNPGKGIEKPAD
                     LDDPAKTARLRNVYNAMVDLEIDALISIGGDDTLKTANFMYEYQKHLPKGARRVQVIH
                     LPKTIDNDYRGIDFTFGFFTAVDVMAKELQNLRADAIATSSYFIVETMGRKAGWLSYG
                     VAIAGEANLVLAVEDIKGDLTIDGAGKYLSLDALVDLIVDLITTRERRGKHYGTVVLA
                     EGLAEMLPESTIANLPLDEHGHLPLGRFDLGKLVAQLVMKKYEEKKQRKKKLIGLQLG
                     YESRCAPPHAFDVMLGSQLGIGAYRALVEENLDGHMVSVSGQLDLNYVPFKDLINPVN
                     MKTEVRFIKTGSDYHKLARFLETRTDRIQEWSPGRRQEPNDRR"
     gene            7310..8629
                     /locus_tag="VMZ53_12215"
     CDS             7310..8629
                     /locus_tag="VMZ53_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29273.1"
                     /translation="MGARIVDARELDQAPRALVNFGQAPDGLPWLCDVDRSARIDKLY
                     RTIASLFDVEKVDDLPPSVREATQGALARGLMGVHAVVLDQRGGLPAFVTRRTARSLF
                     GPTLLQQMLASKNAPHRLPDSERDPITSVSPIGMSEALEQAIEHSKAGRHVVFTLGGE
                     TWTAMVTDANRSLRVPAAPFRRAVFESLAAVAGTTARIGVGYESWTASLTDTRAYIDA
                     LMRAGEAPNGGWVSYEVPRATVLRILAGVAELAGELHRRGIVHGDLTPSNVILDGARP
                     TSADALHIESGQIAAAATFEWAAPEQVVGRPLDPRADVYAIGRMMCELVGAVPFGEKI
                     EYVVPTGGTKAKTVQLLKTDGVFIDAAALGVNREWQARWQDALGKLVAYDRERRVDSG
                     VAAAQLLGELVEKFPPPGHVSVAGHFGVIAPLARPATFPLARVVTDG"
     gene            complement(8636..9799)
                     /locus_tag="VMZ53_12220"
     CDS             complement(8636..9799)
                     /locus_tag="VMZ53_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29274.1"
                     /translation="MREGYTLWQIAAMRILVALLMIVASTVAAHADAPAHDFIDDARA
                     LLVVGACAEGKAPETVKADVVEAHCKKVRKVQDDYKTSWVTPASEWMATNVPKTVSKT
                     VVYPFAGGDLSTALTVYPDADEITTLSLEPAGDPRALGKLNDKQLKAALKVVETELGS
                     LYKSNFSVTMNMIGAMRGGQLPTQLIFSLSALAIHGYEPVSVKYFKLDKDGAIHYLTD
                     DDLAAIDKIKNVGKHNQELANVELVFQKKGGKKQQTYRHIMANLDDNHLKKDPSALAH
                     LEKKGHVAGMTKAASYLLSFTGFDTIRKYIIDHVDWMISDTTGLPPKYGTPAGFEYEV
                     WGVWGTPNMKAAYGIQGDWKALFKQQTKRELKFRFGYPDMKLNGHIVIMKRKS"
     gene            9798..10577
                     /locus_tag="VMZ53_12225"
     CDS             9798..10577
                     /locus_tag="VMZ53_12225"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HUS29275.1"
                     /translation="MLCRTLLAPLLVLVAVGACDSGHKAPSKERRAPITETRPAEPDL
                     YTTLRNEMVTDTIETRGVHDPKVIAAMRLVPRHAFVPPQVRDRAYEDHAVPIGFDKTI
                     SQPYIVAMMTAAAHVKAGDKVLEIGTGSGYQAAVLAMMGAKVFTIEIHDQLATRTKEV
                     LKKSGFSQVKLRTADGYYGWPEEAPFDAIIITCATPEIPPALLEQLKTGGRVVVPLGE
                     AEQTIISVTKGADGDLRKEIVLDGVIFGPMIGEIENRADDL"
     gene            complement(10574..>11332)
                     /locus_tag="VMZ53_12230"
     CDS             complement(10574..>11332)
                     /locus_tag="VMZ53_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29276.1"
                     /translation="IRLGSLALLSLVAACTGEIESDNLEGLPPEEQVARTAWVDKALP
                     IFAENCNSCHGGSMPMIAYVAGTDDLMKRDTLVNYVPRIVNLGAPQSSRVLTKGDHSA
                     TGGGPALLAPQATDILMWILAESRAHPVTALRTAPATAMTGGMMNTVDLSSLGVPATL
                     TFSLDAVGADAYFTNLKLTAGASGLYFEHPLLETNPAGSTEPKPDPIDRFFAVQGNLM
                     PNQSITIGDGTASVAGFNPTDPLSFRFDVFEAKH"
BASE COUNT         1866 a         3670 c         3728 g         2068 t
ORIGIN      
        1 cgggctcgca tcaggcccac gcgcatcagc ggccgatccg gcagcatcgc cgcccacgcc
       61 atcgacgcta ccgccaccac acgccgccag aaggatcgct agaacagcag cgcgcatgtc
      121 ccaattgtag aggaagccgc gatgatcgcg cagatcaaac gcaaccaccc atcccccaac
      181 ctttgcttgc ggcctctgcc ctcgtttgcg cctttgcgtt cctccggaag ggaccgaggc
      241 ggcgcagcag cgcagcggtc agcggccgcg gcggcggcgg cgggcgaaca tcgcgaggcc
      301 gacgaggccg agcagcgcag cgattagcgg cggcgagcga ctagcggcgg cggcggcggg
      361 cgaacaacgc gaggccgacg aggccgaaca gcgcgagcga ttggcccgtc ggtgcgccac
      421 cggcgtcgca acagccggcg tgcatgccgc caccatcgtc ttcgccgttg ggcgggacgc
      481 acatgccgtc gtcgcagacg tcgccgttgt cgcagaggtc agcgcccggc tcgtcggttt
      541 gaccgtcgca atcgttgtcg acgccgtcgc agacttccgt cgaggcggat gccggcggag
      601 cggtgcagcc ttcccagttg ccggcgtggc aggtttcggt gcccgcgccg caggcgttcg
      661 agcatgcgcg cgtgaggtct tcgtcgactt tgccgtcgca gtcgttgtcg aggccgtcgc
      721 aagtttcaga ggagaccgag ccggcgatct ggttgagcgc ggtgagcagc tcttgcgcgt
      781 tgtcgacttg gaagtagcag ttgttcgcgg cggccgcgtc ttcgctcgtc gggttgcagc
      841 ccgtcttcgc ggtgttcgcg gcgacggcca tcttgttgag gccgagcgca tcgacttcgc
      901 caccgaagcc gacgatccac gtcgtgacgc ccttggcgtt gagcgcggcg atcgcgtcga
      961 cgccgtcgaa gcgagtgccg ttgtcgtacg gcacgcagta ctgccagccg tcggtgatga
     1021 ggacgacgtg ctttggaccc gtcacgctca tcgcggagag gttcgcggcg gcttcgagcg
     1081 attgcgacat cggcgtgtag ttgccggacg tcggcggcgg cgagccgagc gcgctgatga
     1141 tcgaggcctt gttgttgagc gcgggggcga cgtcgagcgc gccgggaccg cattggttcg
     1201 gctgcgggaa cgtcatcagg ccgaactcgg ccttcgtctg gtactgctcg aggagcttgc
     1261 cgaggccgtc ggcggccgtg tcccacttgg tgacgccgtt gatctggccc gtcaccatcg
     1321 acgatgactt gtcgaggacg accaggacgc gcgcgggcgt gcattggtcg gccgccgccg
     1381 tgtgggcggt cgcgaggagg ggaagcgaga gggcggcgag cgaggcgaag gtacgaagca
     1441 tggcgtgccc gcgccatgag caaccggggt gccatcgatt tgtcgacgac gatcggccga
     1501 tcacgcgtac ttggaagtgg ctagtcgagt agccacggcg gatcgatccg tcgcgaaaac
     1561 cagtttgcag ttaccaactt gtgcacgttt ctagggtcgc gtgaccctag cgaggcacga
     1621 gttggcgcag ctgccaggct tggagctgga ggctgtgggc agcggtgagc gcgcgcgttc
     1681 gcatctggta gcccgcgaag atcgacgcgc cggcgaggca tgccgcgtag agcgcagcga
     1741 ccacgatcgt cggggtcgac cgcgcgccat cgagcgccgg gccgctgaag acgacgccgt
     1801 gcggcgtgac ggtcatcgtc gtcgagacga tgccgagctg ctcgagcccg agcgggacga
     1861 gcatcgcgcc gaccatgagg gcgccgatcg tgatcggcga gccgagccac gagaagcgcg
     1921 gcgtgagcac catcgccatg ccgagcgacg cggcaacacc gggcgcgatc aagatcggcg
     1981 agaacatgcg cgcgaccgcg atgacgatca cggtgttcgc gatcacgacg aggcctggac
     2041 gaggcttgac cgcgcgcacc gcgacgaccg caacgacgcc atcgatcgcg agcagcacgg
     2101 cgagcaccca cattccgttg acgctgccag gcgccatcca ccacacgagt ggcaggaaga
     2161 gcagcgagcc aatgacggcc catacgcctg ctttcgcaat cgccttggcg tcatgcacgt
     2221 cgacggcagt catcgcttct ttgacctcga tcggcgtctc gcgcggcggc tcgagcatca
     2281 gccgaccgac gagctcggcg gcgcccgaca gtgcgggatc gagcgcgagc gcgccggcag
     2341 cttcacgcat cgcggtgcgg cggttttctt cgctgtcgtt ggcgaacgca gcgcgtgcgc
     2401 gatcgagatg ctcgcgtgcg aggttgcggc gcagcgcgag gtcgcggtca ccatcgagga
     2461 acctctcgac tcgttcgccg agctcgcgtg cagtctgcgt gcgattcgcg cgcgcgacag
     2521 ccgttgcctt cacgcacatc tcgtcgagct cgggcgcgat ctctcggtcc ggcgcccgga
     2581 cgctcggccg cgcgtcgatg tccgtgagcg cagactccat accgagctga ccgcgtggat
     2641 gcagcggctc accagagagg atctcgaaca gcaggcagcc gagcgtgtag acgtcggcgc
     2701 ggccatcgat gtcggtgagc ccgcggactt gctccggcgc catgtagccg ggcgtgccga
     2761 tcgccgtgcc cgcgcgcgtg acgtgctcgc cactgccgct tccgatgtcg gcgaactcgc
     2821 cgtcttcctc gccgatgatc ttcgcgacgc cccaatcgag gacgtacacc tcgccgtagt
     2881 cgccgagcat gatgttgtcg ggcttgagat cgcgatgcac gatgccgcgc acatgcgcga
     2941 actcgaccgc gagacagacg tcggcgaagg cgcgtagcac gcgttgcagc ggcatgtgag
     3001 cgcggtcttc gagaattttc gcgagagacg ttccggtgag cttcttcatc acgaagtacg
     3061 gcaaaccgtc gctgtcgcgc ccgatctcgt gaaccgggac gatcgcggga tgctcgaggc
     3121 ggccctgcac catcgcctcg cgcaagaatc gctgaattga tttctcgctc gggttcgcag
     3181 cgcgcatgcg cttcaccgcc acatcgcgcg cgatctgctc gtcacgagct gccatcacct
     3241 cgcccatgcc gcctttgccg atgcgacgac cgagacgaaa gcgcccactg acgatcgcgt
     3301 ttggcgcggg ctcgggacga tcttcgccag ccagctcgtc gccggtgata gcgaccttga
     3361 tcgtcggctg gtcggctttg tcagagctca tgactccacg ataaccgaat cacgttcgcg
     3421 cacgacggtg cacaacacac ctcctcctgg ctactccgtt agccacgtgc attcgtcttg
     3481 cagatggtct acgtaccgtg ccctggctta tgaagcgatt ctccttactg gctctctctt
     3541 tcaccgcctg catcgattct tcgtcgacgg atctcgatct cgcctctgcg gatcccgaga
     3601 tcacgagcgc cgagaaggcc gacgcgccca gttgggagaa cgcggcgacg ctgcatgaag
     3661 gcacgcgcat cttcgatcgc gccggcgcca attctcgtcg cgttcactcg ctgtggatca
     3721 gcggcagcaa catgaatcac gtgccgctcg tcgtcgacgc gcgcgcgacc gacgcgaacg
     3781 atgtccgcat cgcggtgctc ggtccgctcg tcaacggcgt ccgtcccgtc ctcgcagccg
     3841 atggctacgc acaacgcaag cgcgacgcga gcgtgacgct cgatatcagc aaacccggcg
     3901 agcacctcgt cgtagtcggc tcgtacaacc tcgcgagcga gacgttctac gatcttcatg
     3961 cgacgtgccc gggccccgat ggctgcggtg tctcgcgcta cgacgtgctc gcgactccga
     4021 aggacggcgc gctcgtcggt gattcgacgc gcctcgtctc gatgctgctc ggcgacgtga
     4081 tggtcggcca ttcgtacgac gtcaacgtcg agctctgggc atcgccaccg atgcagccgt
     4141 ggaacgcgac aaaggttggc acgagcacgg cgtcgggcac acaggtcaac gcgatcattc
     4201 cggcgtcggt ccacgaaggc gatgacctca agctcgtcgt tcgccaagcc ggcggccgcg
     4261 tcctcgacac cggcgtcacg acgcgcttct tcccgaaccc gcactcgttc gcgcgcctcg
     4321 actcgatcct ttacggcgac atcgcctcgc tgcagatcgc aggcgtcgtc ggctacttcg
     4381 agggcgtcgc cgatatgcga ctcttctcgg agacgcgcga gatggagctc gcacacacca
     4441 cgcttcacgc ggatcgcccc ggccaggaag gcaacggcta caactcgttc gacgcgacgt
     4501 tccttccgga ctacgcgacc aacgccagcc tcgctccgca cgacggcgaa atcctctcgg
     4561 tcggctacat caacggcaac ggcgactacc gtcggctcgg ctgcttcgag tactgcaaca
     4621 atctctcggg tctctcgagc tgcaccggcg gcgcgcgctc ctgcccataa gcgctaagct
     4681 ccggccgtgg agcaggaccc gttcacgctc gtcggcggag ccgacaagat ccgcgcgctc
     4741 gtcgagacgt tctacgatgt gatgagcgaa cgcgagcccg ctctcgcgcg ccttcacaaa
     4801 tgcgatcccg acggcaaggt ggaccgcggc tcgcgtgatc ggttcgcgct gtttctcatc
     4861 ggctggctcg gagggcccga cgattacgtc gagcagcacg gccatcctcg gctgcgcatg
     4921 cgtcacggcc gcgttgctgt gaacctcgcg atgcgcgacg cgtggctgcg ctgcatgcaa
     4981 gtggcgatgg accagcacgc gatcagcggc gacgttcgca cgttcctcga cgcgcgcttc
     5041 gccgaggtag cggacttcat gcgcaacgtc gacggctaag aacagcaata caacgctgta
     5101 gcgcacgtga cccgtgctcg tccttgttgt ccacgtccag gtccagcgtc cgcgtgtctc
     5161 gtgctcgtgc tcgtccacgt ccacgtccac gtgtcacgtg ctcgtgcacg tccacgtgtc
     5221 tcgtccacgt ccacgtccac gtgtcacgtg ctcgtgcacg tcccacgatg cgtccacgtt
     5281 ccttccgcgc gagctcagcg gcacatcttg ctcagcatct cgacgatgcg cgtaatgcac
     5341 cgcttcgcat ccgcaatgtc cgagtccgaa gcaattcgca gaaatcgaat gacgtcgagg
     5401 atggcaccgc actccatcgc ctcgccacga gcaatcgcat agcggttctc gcggtccgcg
     5461 gcggtcttct tgccgaccgc ttccgcgatg ttcagtggaa tcgacatggc ggcgcgcttc
     5521 cactgatcgc tgacggccga gtggccacgg ggaatcttgg ggacgacctt gaagacgaac
     5581 gtaaggtgtt cgatcgagca ttggtagacg tctagctttt cgtagtcgag cattgcgtgg
     5641 gttgtcgacc gaactgcaga tcggttgcgg tctggccgag cgtgcggcca agtccgcgtt
     5701 ctcattcgtg gacgtgcacg tgcacgtgac acgtggacgt ggacgtggac gtggacgtgc
     5761 acgtgcacgt ggcacgtgga cgtggacgtg cacctcgacg gcgcacgaca acgtggacgc
     5821 gagcggactc ggtcctcgtg tcgctcgcct tcttcgcttt ttgtgtttta ccggcggtcg
     5881 ttcggttctt gccggcgtcc cgggctccac tcctggatgc gatcggtccg cgtctcgagg
     5941 aaccgcgcga gcttgtggta gtcgcttccc gtcttgatga agcgcacctc ggtcttcatg
     6001 ttgaccggat tgatcaggtc tttgaacggc acgtagttga gatcgagctg gcccgacacg
     6061 ctcaccatgt ggccatcgag gttctcttcg acgagcgcac gatacgctcc gataccgagc
     6121 tggctgccga gcatcacgtc gaacgcgtga ggcggcgcgc agcgcgactc gtagccgagc
     6181 tggagaccga tcagcttctt cttgcgctgc ttcttctcct cgtacttctt catgacgagc
     6241 tgcgcgacga gcttgccgag atcgaagcgg ccgagcggca agtggccgtg ctcgtcgagc
     6301 ggaaggttcg cgattgtcga ttcgggcagc atctcggcga ggccttcggc cagcacgacc
     6361 gtgccgtagt gcttgccgcg gcgctcgcgc gtcgtgatca gatcgacaat aagatcgacg
     6421 agcgcgtcga gcgagaggta cttgcctgcg ccgtcgatcg tcagatcgcc tttgatgtct
     6481 tcgaccgcga gcacgaggtt cgcctcgccc gcgatcgcga cgccgtacga gagccagcct
     6541 gctttgcggc ccatggtctc gacgatgaag tagctgctcg tcgcgatcgc gtctgcgcgc
     6601 aggttctgca gctccttcgc catcacgtcg accgcagtga agaagccgaa cgtgaagtcg
     6661 atgccgcggt agtcgttgtc gatcgtcttc ggcaggtgga tgacttggac ccggcgcgcg
     6721 cccttcggca ggtgcttctg gtactcgtac atgaagttcg cggtcttcag cgtgtcgtcg
     6781 ccaccgatcg agatcagcgc atcgatctcg agatcgacca tagcgttgta gacgttgcgc
     6841 aagcgcgcgg tcttcgcggg gtcgtcgagg tcggcgggct tctcgatgcc tttgcctgga
     6901 ttcgtacgcg ccgtgccgat cacgatgccg cgcgtgttgc gaagccccga gaggtccttg
     6961 aggttgaaga tgcggtagtg ctcgtccggt aggagacgat gcgtgaccgg gtggtaaccc
     7021 tcgaggttcg agtagccgtg gaagaagccg atgcactcgc ggttgtcttc cagaaacgag
     7081 atcgcagccg acgaaatcac ggcgttagca gcaggcgcgg gcccgccgga gaacaccaac
     7141 ccgacgcgct tgatgtggtc gcccttgcgg ctcatgtgag gcagacccta cctccggctg
     7201 tgcggtgcgt ccatgtcgga gggcatcatc acgatggact ctcctggcac gctcagcagc
     7261 gatctgggac gctggcgtga tgacctcgat cccgcctctt cgttcggccg tgggagcacg
     7321 catcgtcgac gcgcgcgagc tcgatcaggc gccgcgtgcg ctcgtcaatt tcgggcaagc
     7381 cccggacggt ttgccatggc tgtgcgacgt cgatcgatcg gcgcgtatcg acaagctgta
     7441 tcgaacgatt gcgagcttgt tcgacgtcga gaaggtcgac gaccttcccc cgagcgtgcg
     7501 ggaggcgact caaggcgcgc tcgcccgcgg cctcatgggc gtgcacgctg tggtgctcga
     7561 tcagcgcggc ggtctgccgg cattcgtgac gcggcgaacc gcgcgatcgc tgttcgggcc
     7621 gacgctgctg cagcagatgc tcgcgtcgaa gaacgcgccg catcgccttc ccgatagcga
     7681 gcgcgatccg atcacgagcg tgtcgccgat cggaatgagc gaagcgctcg aacaggccat
     7741 cgaacattcg aaagcgggac gccacgtcgt cttcacgctc ggcggcgaga cgtggactgc
     7801 catggtgacg gatgcgaacc gctcgctgcg cgtacctgcg gcaccgtttc gccgtgcggt
     7861 cttcgaatcc ctcgctgctg tggccggaac gactgcacgc atcggcgttg gctacgagtc
     7921 gtggaccgcg tcgctcaccg atacgcgcgc gtacatcgat gcgctcatgc gcgccggcga
     7981 ggcgccgaac ggcgggtggg tgagctacga agtaccgcgc gcgacggtgt tgcgcattct
     8041 cgctggcgtc gcggagcttg ccggagagct gcaccgccgc ggcatcgtgc atggcgacct
     8101 caccccgagc aacgtgatcc tcgacggtgc gcgcccgacg tctgccgatg cgctgcatat
     8161 cgagagcgga cagattgcag cggcggcgac gttcgagtgg gcagcgcccg agcaagtcgt
     8221 cgggcgtcca ctcgatccgc gagccgacgt gtacgcgatc ggccgcatga tgtgcgagct
     8281 cgtcggcgcg gtgccgttcg gcgagaagat cgagtacgtc gttccgaccg gcggcaccaa
     8341 ggccaagacg gtccagctgc tcaagaccga cggcgtgttc atcgacgcgg ccgcgctcgg
     8401 cgtcaatcgc gagtggcagg cgcgttggca ggatgcgctc ggcaagctcg tcgcatacga
     8461 tcgcgagcgg cgggtcgatt caggcgtcgc tgcagcgcag ctcctcggcg agctcgtcga
     8521 gaagtttccg ccgcccggtc acgtcagcgt tgccggccac ttcggcgtga tcgcgccgct
     8581 ggcgcggcca gcgacctttc ctctcgcgcg cgtcgtgacg gacggatgat cgcggctagc
     8641 tcttccgctt catgatcacg atgtgaccgt tgagcttcat gtccggatag ccgaaccgga
     8701 acttcagctc gcgcttcgtc tgctgcttga acagtgcctt ccagtccccc tggatgccgt
     8761 acgcggcctt catgttcggc gtgccccaga cgccccacac ttcgtactcg aagcccgcag
     8821 gcgtgccgta cttcggcggc agccccgtcg tgtcggagat catccagtcg acgtgatcga
     8881 tgatgtactt gcggatcgtg tcgaagccgg tgaacgagag cagatagctc gccgccttcg
     8941 tcatgccggc gacgtggccc ttcttctcga gatgcgccag cgcgctcgga tccttcttga
     9001 gatggttgtc gtcgaggttc gccatgatgt ggcgatacgt ctgctgcttc ttgccgccct
     9061 tcttctggaa gaccagctcg acgtttgcga gctcctggtt gtgcttgccg acgttcttga
     9121 tcttgtcgat cgccgcgaga tcgtcgtcgg tgaggtagtg aatcgcgccg tccttgtcga
     9181 gcttgaagta cttgacgctg accggctcgt agccgtgaat cgcgagcgcc gagaggctga
     9241 agatgagctg cgtcggcagc tgcccgccgc gcatcgcgcc gatcatgttc atcgtcaccg
     9301 agaagttcga cttgtagagc gagccgagct ccgtctcgac gaccttgagc gccgccttga
     9361 gctgcttgtc gttgagcttg ccgagcgcgc gcggatcgcc ggcaggctcg agcgaaagtg
     9421 tcgtgatctc gtcggcgtcg ggatagaccg tcagcgccgt cgacaaatcg ccgcccgcga
     9481 acgggtagac cacagtcttg ctcaccgtct tcggcacgtt cgtcgccatc cactcgctcg
     9541 ccggtgtgac ccagctcgtc ttgtagtcgt cctgcacctt gcggactttc ttgcaatgcg
     9601 cttcgacgac gtctgccttc acggtctcgg gcgctttgcc ctccgcgcat gcaccgacga
     9661 cgagcagcgc acgcgcgtca tcgatgaagt cgtgagctgg ggcatctgca tgagcggcta
     9721 ccgtcgacgc gacgatcatc agcaaggcga cgaggattcg catggccgca atctgccaca
     9781 gcgtgtagcc ttcgcgcatg ctctgccgca cgctcttggc gccactcttg gtgctggttg
     9841 cggtcggcgc gtgcgattcg gggcacaaag ccccatccaa agagcgacgc gcgcccatca
     9901 ccgaaacgcg gcccgcggag cccgacctct acacgacgct gcgcaacgag atggtcacgg
     9961 acacgatcga gacgcggggc gtccacgatc cgaaagtcat cgcagcgatg cgcctcgtgc
    10021 ctcgtcacgc gtttgtgccg ccacaggttc gcgaccgagc gtacgaggac cacgcagtcc
    10081 cgatcggttt cgacaagacg atcagccagc cgtacatcgt cgcgatgatg accgctgctg
    10141 cgcacgtcaa agcgggcgac aaggtcctcg agatcggaac cggcagtggc tatcaggcag
    10201 ccgtgctcgc gatgatgggc gcgaaggtct tcacgatcga gatccacgat cagctcgcga
    10261 cgcgcacgaa agaggtgctg aagaagtcgg gcttctcgca ggtgaagctg cgcaccgccg
    10321 acggctacta cggctggccc gaggaagcgc cgttcgacgc gatcatcatt acgtgcgcaa
    10381 cgccggagat tccgcccgcg ttactcgagc agctgaagac cggtggtcgc gttgtcgtgc
    10441 cgctcggcga agcggagcag acgatcatct cggtgacgaa aggggccgat ggtgatctgc
    10501 gcaaggagat cgtcctcgac ggtgtgatct tcggaccgat gatcggcgag atcgagaacc
    10561 gcgccgacga tctctaatgc ttcgcttcga acacgtcgaa ccggaagctc agcgggtcgg
    10621 tcgggttgaa gcctgccacc gacgcggtgc cgtcgccgat cgtgatcgat tggttcggca
    10681 tcaggttgcc ctgcacggca aagaagcgat cgatgggatc cggtttgggc tcggtggagc
    10741 cggccggatt cgtctcgagc aacggatgct cgaaatacag gccgcttgcg ccggcagtga
    10801 gcttgaggtt cgtgaagtac gcgtcggcgc cgaccgcgtc gagagagaac gtcagcgtcg
    10861 cggggacgcc gagtgacgag aggtcgaccg tgttcatcat gccgccggtc atcgcggtcg
    10921 ccggtgcggt gcgcagcgcg gttacgggat gcgcgcgcga ttcggcgagg atccacatca
    10981 ggatgtctgt cgcctgcggc gcgagcagtg ctggaccgcc gccggtcgcc gaatgatcgc
    11041 ccttcgtcag gacgcggctc gactgcggcg cgccgagatt gacgatgcgc ggcacgtagt
    11101 tgacgagcgt gtcccgcttc atcagatcgt cggtgccggc cacgtacgcg atcatcggca
    11161 tcgagccgcc gtggcacgag ttgcagttct ccgcgaagat cggcaaggcc ttatcgaccc
    11221 aggcggttcg ggcgacctgc tcctccggcg gaagaccttc tagattgtcg ctctcgattt
    11281 cacccgtaca ggcggccacg agcgacagca gcgcgaggga ccccaggcga at
//
LOCUS       DATLBZ010000230        22838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5455506, whole genome shotgun sequence.
ACCESSION   DATLBZ010000230 DATLBZ010000000
VERSION     DATLBZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22838
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5455506"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VMZ53_12235"
     CDS             complement(<1..1257)
                     /locus_tag="VMZ53_12235"
                     /inference="COORDINATES: protein motif:HMM:NF017541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HUS29277.1"
                     /translation="MFDFTKHFSTKTTSQSEPIPGTNQVPNSAGGFTWKLNDWARLDR
                     FLVLGSEAGTFYVGERELTRENAAVVARCIAADGIRTIHRIVAISTTGRAPKNDPAIF
                     SLAMASKLGDEPTRRAAYAAVPKVCRIGTHLMHFAEYAQAFGGWGRGMRRAIGAWFNA
                     RPATELALQLAKYQSRDGWSNRDLLRLAHPRAASPSHDRLFHWAVSGELPIDANDDEA
                     LQLIVAMHALRSMTDVPTAAWLIRDKKLPRECVPTELLVHPEVWDALLEAMPMTAMIR
                     NLATMTRVGLLTQTSSATKRVVEQLRDAARLQRARIHPLAVLAALMTYKSGRSVRGSS
                     SWQPVAQIVDALDAAFYASFAAVEPSNKRTLLALDVSGSMHGGIVGGVVGLTPRIASS
                     AMALVTAATERDHSFTAFTDGITPLSI"
     gene            complement(1375..1446)
                     /locus_tag="VMZ53_12240"
     tRNA            complement(1375..1446)
                     /locus_tag="VMZ53_12240"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1412..1414),aa:Thr,seq:cgt)
     gene            complement(1591..1664)
                     /locus_tag="VMZ53_12245"
     tRNA            complement(1591..1664)
                     /locus_tag="VMZ53_12245"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1629..1631),aa:Lys,seq:ctt)
     gene            complement(1671..1743)
                     /locus_tag="VMZ53_12250"
     tRNA            complement(1671..1743)
                     /locus_tag="VMZ53_12250"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Phe,seq:gaa)
     gene            complement(2287..3276)
                     /locus_tag="VMZ53_12255"
     CDS             complement(2287..3276)
                     /locus_tag="VMZ53_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HUS29278.1"
                     /translation="MRAVAIVNGNARRLRGRLRSRLEKALPGRVRFTESLDEARTTIR
                     AEVKRGAELIVLGGGDGTVVMGLTLIGEACRGAGKAEPSIGILRLGSGNAIADTLGAG
                     DDVVEDLERLVRGGGRRRTVPMIEVLGVRAPFVGMGVDALLLEDQEAVARVVDRVPVA
                     RRLLGGASRYALSVALRSVPRFAAGARPNAVVTNLGGPAIEMARSGPTGREEPAGQII
                     WKGACTLVAGATIPFFGFGLKMFAFANRREGKFHLRCGDAGLFEIMRNTPAAFRGDYF
                     SDNVTDYLCDRVAIELDQETAIEAGGELLGRRNHVELAIAPAITMLALENSPR"
     gene            3305..4252
                     /gene="mtgA"
                     /locus_tag="VMZ53_12260"
     CDS             3305..4252
                     /gene="mtgA"
                     /locus_tag="VMZ53_12260"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HUS29279.1"
                     /translation="MPPARKRRSSRAGTPPPVKPTRRWAARILLAIEAVVIATGLIAF
                     AIYASLPNTRRLATENPTSTAFIDLRRDQAAGKKDWKLEWKWRPIGKISRYLRAAVIY
                     AEDASFYRHDGVDWHAIEQAFDKNLEAGELSVGGSTITQQLAKNLYLSPSRNPIRKLR
                     ELLIAFSLEDHLTKTRILELYLNVVEWGDGVFGAEAASQHWFGHSAQTLSPSEAARLA
                     IALPNPFKRNPTVQTEELRKKAVRIVRLLRMQGLINASQERQALDDVGAQSAVVRPDK
                     SLPPAPPPQTAPPQTRPPETPELETPEETDPMPPEAPPE"
     gene            complement(4256..5005)
                     /locus_tag="VMZ53_12265"
     CDS             complement(4256..5005)
                     /locus_tag="VMZ53_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS29280.1"
                     /translation="MKWSTEHGMVVRRTGGDGPRIVWIHGLGESSVSFEPVVAKMPAF
                     SHVMIDLPGYGRSAWPDAATNLETLADHLASWLSSAAPAFLVGHSMGGVLATLVAERA
                     TVAGIVNIDGNISSGDCTFSAKASAYSLADFRDQGFGEMRAQVYADGMQRPELRTYHA
                     AMCLAHPESFHRHAGDLVEMSATNTLGPRLAALTCPALFVAGVPDGVCVETRRQLTEL
                     KVRWVGIEPAGHWVYLDQLDKFVAELSRFLR"
     gene            complement(5005..5472)
                     /locus_tag="VMZ53_12270"
     CDS             complement(5005..5472)
                     /locus_tag="VMZ53_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HUS29281.1"
                     /translation="MKRLALVLVLAACGGDDGGGDDGGGGADPAHQACVDETNTYRAM
                     KSKPAVAWSMELEAYADTGAQDDFSSSPHHHFSSTSGGGIAFAENECPQQGNWQLPAG
                     GDMVALVKQCVKAFYDEGPGGGHYENMMGPYGKLGCGIYQSGTKVTIVQDFGN"
     gene            complement(5472..6704)
                     /locus_tag="VMZ53_12275"
     CDS             complement(5472..6704)
                     /locus_tag="VMZ53_12275"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HUS29282.1"
                     /translation="MARINEHYAKLPASYLFPEIGRRVRAYQAANPDAKVIRLGIGDV
                     TEPLAPAIVEAMHAAVDEMGKRETFHGYGPEQGYDFLVEAIRQHDYASRGVDIAADEI
                     FVSDGSKCDSGNVQEIFALAAKIAVTDPVYPVYVDSNVMAGRTPAAGGDGRYPGLVYL
                     VGNEHNGFQPDPPSEKVDVVYLCSPNNPTGTVATRAQLERWVAWAKQNDSIILFDAAY
                     ESYISDSSLPHSIYEIPGARDVALELRSFSKRAGFTGVRCAFMVVPKTVTGTNPAGER
                     VSLNALWARRHTTKFNGASYVVQKGAAAAYSAEGSKQTNAQIAFYMENARLLREGLTA
                     SGFTIFGGVHAPYIWIRTKNAATSWEFFDQLLSKAQVVGTPGSGFGPAGEGYFRLSAF
                     NSRANIEEALSRIKKVFS"
     gene            6761..7150
                     /locus_tag="VMZ53_12280"
     CDS             6761..7150
                     /locus_tag="VMZ53_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29283.1"
                     /translation="MKWLLVVALTGLVAGCAHDVAAQFPSQPTEPSGTLVLQLTRPAS
                     GVTVAINGVLVVDDEHTQRVVIAGVPSGNTEVVMAANGGDKAFRVWIDSARPTTVPLG
                     VPDEGSGFLKSVAGSILSIVVYSLLHR"
     gene            7207..8100
                     /locus_tag="VMZ53_12285"
     CDS             7207..8100
                     /locus_tag="VMZ53_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29284.1"
                     /translation="MTRLWTTLTLCLAATSPAFAQSAGSDDDSNQAGPVETNTNADGL
                     TREGNVQDRTGTDTDNPSDSTAPTNEQVDTMDPNNTQNTSSDETMQGTSEQPGYVVAS
                     TRSTTIEQSSERERLGIAIAAGAGAGGFTSDALRGSAGLGGDWDVRLTLGTRSPLAIE
                     ASYIGSAQRLSALGLENNAVLVGNGAQGALRLNGTIDFPVQPFIFGGLAWRRYDVTNS
                     AANTSDVAETDDVLEVPAGAGLAGKVSGLIVDVRGEFRYTMNADLVRTGNVDTPVIGV
                     SDNEFGTMHRWGINANVGYEF"
     gene            8152..8607
                     /locus_tag="VMZ53_12290"
     CDS             8152..8607
                     /locus_tag="VMZ53_12290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HUS29285.1"
                     /translation="MRALGLLALAACGGTADSTIDVTHDPCAPLALVSADVTPSETAG
                     MQGAQKLWREHGAPALGLRAGATLEVRFDDASPAFHGLYDDEAGVIYINRSIQDETTL
                     SIVIAHELGHAFGLEHVAGRTSVMNAGNLKVEPNDEDRSALGNLWGSCN"
     gene            8720..9085
                     /locus_tag="VMZ53_12295"
     CDS             8720..9085
                     /locus_tag="VMZ53_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS29286.1"
                     /translation="MHRVMVVDDEPDTVEVLSTLIEILGYEPRVAKSGKEAIELASVF
                     DPHVVLLDIALPDMSGYDVVQQLRADTARRRFMVAATGYALERDRVRALEAGFDQHLP
                     KPATVQKVREVMQIASRYV"
     gene            complement(9057..10772)
                     /gene="treZ"
                     /locus_tag="VMZ53_12300"
     CDS             complement(9057..10772)
                     /gene="treZ"
                     /locus_tag="VMZ53_12300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HUS29287.1"
                     /translation="MAVRIWAPRAQRLDVVVGETRVAARQDADGWWEGPELAEGDRYV
                     LSIDGAAPCPDPRSRWQPDGVNAASCWVEPQPAPRGHFHPAALRDSVIYELHVGTFSQ
                     EGTFEGAVPYLDHLVQLGITHVELMPVAQFSGTHGWGYDGVDEFAPHAAYGGPRGLRE
                     LIRQCHLRGLGVIIDVVHNHFGPEGAYWTQFGPYTTSKHKTPWGEAINLDDTGSNEVR
                     RFFIDSALAWLRDYGADGLRLDALHSLKDTSERHFVAELVDEVRTLEHQLRRRIVLIG
                     EYDDHDPSAVAPRANGGWGLDAHWNDDFHHAIHVLVANERAGYYGDFAAPGTLAKILE
                     DGYALDGGFSKFRQTRHGKPFGQLPRDRLVAYIQSHDQVGNRALGERLHHLAGNEKTA
                     LAAAILLTSPFVPMLFQGEEWAASTPFFYFAQLESEELRKAVREGRAAEHGGAGWSKP
                     PPDPTQPSTRDASVLRWDERSEPEHAQMLGWYQSLIAARKEYFALRDPRPSSTRVEHD
                     GDVLAIHRGDFTLLCNFADEPRRAQVANTIAATAGLANSRELPPTSCALVAPASVRRS
                     EKQSA"
     gene            complement(10813..11877)
                     /locus_tag="VMZ53_12305"
     CDS             complement(10813..11877)
                     /locus_tag="VMZ53_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS29288.1"
                     /translation="MLHELLTRFRDEIMIRCERKLRARHPQRPAEEVVDTIPLFIDEL
                     IKAERREGGFAEDTPLPVHVPHAKEHGAQRYHNGFDISEIAMDFGSISDTIGEIAIEQ
                     DFYLDPRSYKLLNECIDKAIAESLEQYFALDRASHEEGVAVWIGSLGHELRNALGSAL
                     MAYNVLKAGTVGTASRTARVLERSLQRLHVLITETLATVQLRGGKQLFRQKLYLHELL
                     DDIIQAIPNDRRVSIHNLVEQALYIEADATLLESALSNLILNAVKFTRPGGHVDVRAV
                     AMQGATLLEIEDECGGLGTGNPDELFTPFFQTNPGRGGVGLGLAITREAVAAHGGDIT
                     VRDLAPKGCVFVVRLPQPSP"
     gene            complement(11907..12641)
                     /locus_tag="VMZ53_12310"
     CDS             complement(11907..12641)
                     /locus_tag="VMZ53_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUS29289.1"
                     /translation="MHKPTDIGPNRTGVKTSPFDSRKTIQGAEEGTPAAGMADGHVLE
                     VERVRWSREAMPVGTVPPPGTVKGVVKAIVETLEGNVPNVLIDKLGERLAFERTGARL
                     YDALLAKFEAASVHDDGPTRADIEHIRDDERRHFGIVRDAMVQLGADPTAMTPCADIA
                     GVASLGWIQVLGDPRTTLTQALDIMLAVEAADNEGWTLLVELAETMGFADLARQFRVA
                     IAEEEEHLVLVRTWASTAILGQSRAG"
     gene            complement(12649..13815)
                     /locus_tag="VMZ53_12315"
     CDS             complement(12649..13815)
                     /locus_tag="VMZ53_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29290.1"
                     /translation="MALDIRKEKGVPLDRQVFTWRELAGPPFSKLDDDAFTRVRVILM
                     NGVESEAVRFSHSCARMNRDLRGPLARVRRIEHLQQTLVNWLNPADQSPLETTIGFEQ
                     TAIEITAWVAQQEPDPYLAQVYRFGLLEDFDHLYRFSALMDRMSGKDANNILQSYTDV
                     RPGRPTADEHRAPEDDIRDSYAKLTAAPLSKMHALTIVSAEHQVHDYYMNIGPSFADP
                     VARMLYAEIAAIEEQHVTQYECLIDPSESWLEKWLLHECNEVYTYWSCLGQEPNPRLK
                     KIWERMLDYELGHLHYVMDLVRQIEKRDPEELLPPTLPDPIQFASQREFIRKTLHAER
                     DLRAAGTLFVPRDAEPEGSLQYRQQLNSSGSPSELVSTDYVYTPGTELERMTIH"
     gene            complement(13932..14369)
                     /locus_tag="VMZ53_12320"
     CDS             complement(13932..14369)
                     /locus_tag="VMZ53_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29291.1"
                     /translation="MRLALLVVVGFVVGCGGAPARQAPPKELPVERIGDLAGRWVTSD
                     DMDWSYEMTIDTKGGIDVWIDRGKMGRCEQKGTIVALADRLVRVIYTRGECNPQAVKV
                     PIDMKVTSFTGQSLTVEVANQARTYQRASDDSDGKQPAVQLQP"
     gene            14406..16640
                     /locus_tag="VMZ53_12325"
     CDS             14406..16640
                     /locus_tag="VMZ53_12325"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HUS29292.1"
                     /translation="MASERNDFIRDIIDEDLRTGRHTRVATRFPPEPNGYLHIGHAKA
                     ICVDFGVARDYNGTCNLRYDDTNPTKEDVEYVESIERDVRWLGFAPTAVLFSADYFPK
                     MYELAERLVREGKAYVDDLPDEQIKEYRGTLSEPGKPSPFRDRSVDENLDLLRRMKAG
                     EYPDGARVLRAKIDLASANMKMRDPLLYRIRHAHHHRTGDAWCIYPMYDYAHPLEDAI
                     EGITHSICTLEFENNRELYDWVIDNTGPWNPRPRQYEFARLALEYTLMSKRKLRQLVE
                     EGHVRGWDDPRMPTLAALRRRGYSPEAIRAFCDMIGVAKANSMVDIGKLEYCVRDDLN
                     QNAPRVMGVLRPIEVEIAGDVGAGATDAPYFPPDVGKPGGRALSISNRIFIDRDDWSD
                     DPPADYKRLAPGRTVRLRHGVCITAEEVVSRDAAGAPTKIRAKVTDQGKGAGVIHWVD
                     AATAVRAEVRIYERLFKVPKPEEGGGDWMEHLDRDSLHVVADARVEASLASAAVGSRW
                     QLERVGYFVVDDDSKPGALVLNRIITLRDDKPAVVAAAPVEKKQNVKAATRPKSKSPT
                     EYRAEARARDPELARAYREAEGLGLPAEQADLVSGEPALARLFLAAATKDNAAIVAKW
                     MINELPRVDAETKIEPARFKEFIELVASGALATAGAKSVLADMVKTGKRAGELAASAG
                     QAISADELGTRVQSVIEANPDKAAQYRSGKTGLLGFFVGQVMKSAPNADAAEVNRVVR
                     ERLG"
     gene            16747..17508
                     /locus_tag="VMZ53_12330"
     CDS             16747..17508
                     /locus_tag="VMZ53_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29293.1"
                     /translation="MAALLAGCGARLGGDDQQGATTDANGSNGSNGSNGSNMQMDASV
                     DAPPACANGRVVFLNFEGVAPLTQAATTDATLNRANWLTNTSATIPPYHQGVAGRATE
                     IQTITDGVKSRLAMTPIKVVTTRPASGPYVMVVFGGQRTADGGTVGTPYTLATSEHDC
                     GDTAKNDVGWVSDTASVSYAQDLAIGAIGWGLGLNGTNDPNDCMCSWANTCNSAAGAC
                     TLSASIASTTSTGNTTCPNQNPQNEVAAFSTGFCQ"
     gene            17569..19110
                     /locus_tag="VMZ53_12335"
     CDS             17569..19110
                     /locus_tag="VMZ53_12335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29294.1"
                     /translation="MIGTTVGSYRVTEKVSVGGMGTVYKAEHTLIGKHAAVKVLHPEL
                     RNNRDVVQRFFNEAKATSQINHPGIVEMFDFGYLPSGDGYIVMEFLEGMSLARRLKRA
                     GKMPEGEAALILRSVCSALAAAHAKNIVHRDLKPDNIFLSPDPESPIGERPKILDFGI
                     AKLNVGNPRGTHATQAGAVMGTPTYMSPEQCKGTGDVDQRADLYSIGCIFFEMVAGRP
                     PFTEKGSGELLGAHLYLEPKTPTQVGAKISREAEALIMQLLQKEPSKRVQSAQELAKK
                     FAELAAHVGLVARATPHPLVEYAADAHATTPDHDAHTSALTSAQTTPAGWMTPTPPGS
                     STSSPTPSGVMPKPTTLSGAASQASIARPRSSRVAIAVAAIAVAMVVGGGVTMALKGK
                     KSKTAPAPIATQPTQPAPTPPTAAASAQPPADKSAVVEQPAGVDTRAGVDKPAVVGTP
                     AVVDKPAVVDKPAVVDKPAVVDKPAVVDKRVVTTKKPKTKAKPPTDAGKGSGSAQKIL
                     IETDI"
     gene            19110..20174
                     /locus_tag="VMZ53_12340"
     CDS             19110..20174
                     /locus_tag="VMZ53_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29295.1"
                     /translation="MRFLLAAFLLAPTIAVAQPATGDRGDAKALMQTGVKLLDAKDYL
                     GALATFKEAYSRYPSAKILLNIGTTLKLLGRDAEAANTYQRYLDSSDADAARSEEVKQ
                     ALVELDAKLGRISIQTTPPDAEVQVGDEWMPAAKAKLVRFAPGDVKLSARKAGFDPAS
                     TTVRAIAGAETMAALTLTATLVKVDDAGLEQQRIEGDLRAHDELPIIDEPSVQRSRVG
                     ALANVHVAVYPKLGSAWLVGGTFDALPRLAIDAALLLGPGLVSDGMATLPPPSFGGYV
                     GASYALMLGKLRPRALVGVPVFASDGARLFVRVGGGAEYAATRHLAVIVELAGEIALN
                     PQNDIRDVAIVPALGVTGRL"
     gene            complement(20171..21034)
                     /gene="ttcA"
                     /locus_tag="VMZ53_12345"
     CDS             complement(20171..21034)
                     /gene="ttcA"
                     /locus_tag="VMZ53_12345"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HUS29296.1"
                     /translation="MAEAARQEQRLYRTVRTTCEEYNLLSAGDRIMVAMSGGKDSYTL
                     FHLLMRLIPRLPFKVEMVAVHLDQAQPNYDGTGLRGYLEATGWPYEILREDTYSVVTE
                     HIDDKSTYCSLCSRLRRGILYTAAERLGCNKLALGHHRDDSLETFLLNLFYSGKLQAM
                     PASYRTDDGRFEVIRPLIDCAERDIAEYAATLAFPIIPCNLCGSQDGLKRDAMTALLA
                     QLEQTNPHVRSIMANALRNVRPSHLLDRDVMKAWAERSPDIRPAAEAPPKKQHAAALP
                     VFSGGKLRVVD"
     gene            21033..21785
                     /locus_tag="VMZ53_12350"
     CDS             21033..21785
                     /locus_tag="VMZ53_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HUS29297.1"
                     /translation="MSGLLLDPGTLVILGIVALVAFTTEGAIGFGGTVLAASIGAQFV
                     NLEVLLPAFVPLNILMSSWLLGRGVKVIAWRMLLTEVAPAVAVGTALGLLLFHLPAKT
                     LLALVFGVFVIGLALLQLTRPADRPLARPLQLGFLGLGGVAHGLFGTGGPMIVYVVRR
                     RLPDKLAFRSTLAVLWLALNAALLANYATSGLYTRTTAHVGAVIALALVPGLVLGNKL
                     HHKLDASKFERIVWIVLLLAGLALAVRSAFAL"
     gene            complement(21786..>22838)
                     /locus_tag="VMZ53_12355"
     CDS             complement(21786..>22838)
                     /locus_tag="VMZ53_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29298.1"
                     /translation="ASSVTANAGHSCAVRDDHGVSCWGNNTQNEIGTITGSISCQASG
                     TQFRCTPTPQALAMPPIDKLGMADQASCAIAMDGTTVYCWGTNGGEVGGPIGNLPSPT
                     VIADRAGSQQLIGGQHHLCSLAANGDVLCAGMNTYGEVGNGSQLAVTTPTTVISAATA
                     IGAGFNHTCAVTDGNVACWGANAKMQADGATTNPGLVSPTLVAGVTNAKAVAAGSEHT
                     CALISDGTVKCWGSNQFAQLGMLTASTTSGAVSLPLSQVTAISAGVNHTCTVDAGGRV
                     VCWGEGYSTPGIDDYLVTVTLQKRALSITSGSYHDCAVVDGGDVYCWGWNAYGQLGTG
                     VVSEMNEPPSRVHLCP"
BASE COUNT         4057 a         7430 c         7395 g         3956 t
ORIGIN      
        1 gatcgagagt ggagtgatgc catccgtgaa tgccgtgaag gagtgatcgc gttctgttgc
       61 cgcggtgacg agcgccatgg cggagctcgc gattcgcggt gtcaggccga cgacgcctcc
      121 gacgatgcct ccgtgcatcg agccactgac gtcgagtgcg agcaacgtac gtttgttgct
      181 cggctcgacg gcagcgaagc tcgcgtagaa ggcggcatcg agtgcgtcga cgatttgcgc
      241 gaccggttgc catgagctgg agccacgcac gctgcgtcct gacttgtagg tcatgagcgc
      301 ggcgagcacg gcgagcgggt gaatgcgggc tcgctggagg cgagctgcat cgcgcagctg
      361 ctcgacgacg cgtttcgtcg cgctggaggt ctgcgtgagc aggccaacgc gggtcatcgt
      421 cgcgaggttt cggatcatgg cggtcatcgg catcgcttcg agaagcgcat cccagacttc
      481 gggatgcacg aggagctcgg tgggtacgca ttcgcgtggc agcttcttgt cgcggatgag
      541 ccaggcggcc gtggggacgt ccgtcatgct gcgcagcgcg tgcatcgcga cgattagctg
      601 aagtgcttcg tcgtcgtttg catcgattgg aagctcgccg gagaccgccc agtggaagag
      661 gcgatcgtgc gaaggcgacg cggctcgcgg gtgagcgagg cgcagtagat cgcgattact
      721 ccagccgtca cgcgactgat atttcgcgag ctgcaacgcc agctcggtgg cggggcgtgc
      781 gttgaaccac gcgccgattg ctcgacgcat gcctcgtccc caaccgccga aagcctgcgc
      841 gtactccgcg aagtgcatca ggtgcgtgcc aatgcggcag accttcggca cagcggcgta
      901 cgccgcgcga cgcgtgggct cgtcaccgag cttggacgcc atcgcgagcg aaaagatggc
      961 cggatcgttc ttgggcgcgc gtcccgtagt tgaaatagca acgatccggt gaatggtacg
     1021 aataccgtcg gcggcgatgc accgcgcgac gacagctgcg ttctctcgag tgagctcgcg
     1081 ctcgccgacg tagaacgtgc ctgcctcgga accgaggact agaaagcgat cgaggcgagc
     1141 ccagtcattt agcttccagg tgaagccgcc cgccgaattc ggaacctggt tggtgcccgg
     1201 aatcggttcg ctttgtgacg ttgttttggt cgagaagtgc ttggtgaaat cgaacatggc
     1261 gaacctttcc ggcgagaagt tagtgagtgc ggtgcgcgcc ttgccttcag cgcacaccga
     1321 cgaacctgcg cggcgagact gctgcctcgc agaacgcgca cacgctggca ctcgagcccc
     1381 cgagaagagt cgaacttcca atccgcggtt tacgggaccg ctgcattacc gttgtgctac
     1441 aggggcaaga tcgagcaggt gaccggagtc gaaccggcct gggcttgtgt ggcgcacaag
     1501 cttgtcacca ggacacacct gcttggtggt ggcgttagcc acctggtttt cgagtcagcg
     1561 tcggcagcga tctgcgttcg tgactctgcg tgggcccggt cggattcgaa ccgacgtttt
     1621 gcctgggtaa gaaccaggtg caatccgagt tttgctacag acccgatcag tcccaccctc
     1681 tggaatcgaa ccagaacctc ccgggcttca gccggacgcg cagaccaact acgcaagagt
     1741 gggatacgag tgctgcacgc gtgctttgca ggcgcacgtg cagcaccaga tcatcatcgt
     1801 catcatcttc agttttcaca gaacgggtcg ttcgccggtc ggggcgtaca caagacgcac
     1861 ctcgggcctt gatgcagtcg ctgttacagc gagcgcacca ctcgcgattc gagatctgca
     1921 ttcagatatc gagtcgcaaa tcgctagttg tattgtcgcg aaaacgaagt ggccgaaaac
     1981 gtcgaagggc cacccggttt cccttgggcg gcccttcagc gcacagacgt ggtgttcacg
     2041 tcatgtacga ggaggacctc cgcaggtacc cgtgtcacgc atatccgggc cataacctgg
     2101 acggacgcag ctaaagggca tctgccacga gggcatctgc gccttcaagg cggcttccga
     2161 ggggttatgt ccgttgagtt tccgcacgac ttgttccttt gacgttaaga cgaataaccg
     2221 tgcgcgaacg atctgtcaaa ctgtttctcg acgagaatta caaaggtgca ttgcgattgt
     2281 gttccgtcat cgtggagagt tttccagtgc gagcatcgtg atcgccggcg cgatcgcaag
     2341 ctccacgtgg ttccgccgac ctagcagctc gccacccgcc tcgatcgccg tttcctggtc
     2401 tagctcgatg gctacgcggt cgcagaggta atccgtgacg ttgtcgctga agtagtcacc
     2461 gcgaaaagcg gccggtgtgt tgcgcatgat ctcgaacaaa ccggcatcgc cacagcgcaa
     2521 atggaacttc ccttctcgcc gattcgcaaa tgcgaacatc tttagtccga atccaaagaa
     2581 tggaatcgtc gcgccggcca cgagcgtgca cgcgcccttc cagatgatct gacccgccgg
     2641 ctcttcacgg cctgtcgggc ccgagcgcgc catctcgatc gcaggtcctc cgagattcgt
     2701 gacgaccgcg ttcggccgcg cgcctgccgc aaagcgaggc actgatcgca acgcgaccga
     2761 cagcgcgtag cgcgacgcac cgccgagcag ccgccgcgca acgggcacgc gatcgacaac
     2821 gcgcgcgacc gcttcttgat cttcgagcag cagcgcatcg acgcccatgc cgacgaacgg
     2881 ggcgcgcacg ccgagcactt cgatcatcgg caccgtccgg cgtcgccctc cgccgcgcac
     2941 gagccgctcg agatcctcga ccacgtcgtc gccggcgccg agggtatcgg cgatcgcgtt
     3001 gcccgaaccg agccgcagga taccgatcga cggctccgcc ttccccgcgc cgcggcaagc
     3061 ctcgccgatc agcgtgagcc ccatgaccac ggtcccgtcg ccgccgccga ggacgatcag
     3121 ctcggcgccg cgcttcacct ccgcccggat cgtcgtacgt gcctcgtcga gcgattccgt
     3181 gaaccgcacg cgaccgggca gcgccttctc gaggcgcgaa cgtaggcgtc ctcgcaatcg
     3241 gcgtgcattg ccgttcacga tcgcgacggc tctcactcgc gcacgatacc actatgatca
     3301 ggcaatgccg ccggcgcgca aacggaggtc atcgcgagcg ggcaccccgc cgcccgtgaa
     3361 accgacgcgt cgttgggcag cgcgcatcct gctcgccatc gaggcggtcg tgatcgcaac
     3421 gggcctcatc gcattcgcga tctacgcgtc gctgccgaat acgcgccgcc tcgcgaccga
     3481 gaacccgacg tcgaccgcgt tcatcgatct gcgccgcgac caggccgccg gtaagaagga
     3541 ctggaagctc gaatggaagt ggcgaccgat cggcaaaatc tcgcgctacc tccgcgccgc
     3601 cgtgatctac gccgaagacg cgagcttcta ccgccacgac ggcgtcgatt ggcacgcgat
     3661 cgaacaagcg ttcgacaaga acctcgaagc cggcgagctc agcgtcggcg gcagcaccat
     3721 cacgcagcag ctcgccaaga acctctacct gtcgccgagc cgcaacccga tccgcaagct
     3781 gcgcgagctc ctcatcgcgt tctcgctcga agaccacctc acgaagacgc gcatcctcga
     3841 gctctacctc aacgtcgtcg agtggggcga cggcgtgttc ggcgcagagg ccgcgtcgca
     3901 gcactggttt ggccactccg cacagacgct gtcgccatcg gaagcggctc gcctcgcgat
     3961 cgcgctgcca aacccattca agcgcaaccc gacggtccag acggaagagc tgcgcaagaa
     4021 ggcagtgcgc atcgttcgcc tgctccgcat gcaaggcctc atcaatgcgt cgcaagagcg
     4081 ccaagcactc gatgatgtcg gcgcgcaatc ggccgtcgtg cggcccgaca aatcgctgcc
     4141 tcctgcgccg ccgccgcaaa ccgcgccgcc acaaacgcgg ccgccagaaa cgccggagct
     4201 cgagacgccg gaggagacgg atcccatgcc gcccgaggcg ccgccggagt agtcgtcagc
     4261 gtagaaagcg ggagagctcg gcgacgaact tgtcgagctg atcgagatag acccagtgtc
     4321 ccgccggttc gatgccaacc cagcgcacct tcagctccgt cagctggcgc cgcgtctcga
     4381 cgcacacgcc gtcgggcacg cccgcgacga acagcgcggg gcacgtcagc gccgccagcc
     4441 gcgggccgag cgtgttcgtc gccgacatct cgacgagatc gccagcgtgg cggtgaaacg
     4501 actcgggatg cgcgaggcac atcgctgcgt gatacgtgcg cagctccggg cgctgcatgc
     4561 cgtcggcgta cacctgtgcg cgcatctcac cgaagccctg gtcgcggaag tccgcaagcg
     4621 agtacgcgct cgcctttgcg ctgaacgtgc agtcaccgct cgagatattg ccgtcgatgt
     4681 tgacgatgcc cgcaacagtt gcgcgctcag cgacgagcgt cgcgagcacg ccgcccatcg
     4741 agtgcccgac gaggaatgcg ggcgccgcgc tcgagagcca gctagcgaga tggtcggcga
     4801 gcgtctcgag attggtcgca gcgtcaggcc acgcggagcg gccgtagccg ggcaaatcga
     4861 tcatcacgtg cgagaacgcc ggcatcttcg ccacgaccgg ctcgaagctc accgacgatt
     4921 cgccgaggcc atgaatccag acgatacgcg gcccgtcccc gccggtgcgc cgcacgacca
     4981 tgccgtgctc cgtcgaccac ttcatcagtt gccgaaatcc tgcacgatcg tgaccttcgt
     5041 tccggactga tagatgccgc agccgagctt gccgtacggg cccatcatgt tctcgtaatg
     5101 tccgccgccc ggaccttcgt cgtagaacgc cttcacgcac tgcttgacga gcgcgaccat
     5161 gtcgccgccc gccggcagct gccagttgcc ttgctgcggg cactcgttct cggcgaatgc
     5221 gatgccaccg ccgctcgtcg agctgaagtg atggtgcggc gatgacgaga agtcgtcctg
     5281 cgcgcccgtg tcggcgtatg cctcgagctc catggaccac gcgaccgccg gcttgctctt
     5341 catcgcgcga tacgtgttcg tctcgtcgac gcacgcctga tgcgcgggat ctgcgccgcc
     5401 gccgccatca tctccaccgc cgtcatcacc accgcacgct gcgagcacga gaacgagcgc
     5461 gagtcgtttc atcagctgaa taccttcttg atgcgcgata gcgcttcctc gatgttcgca
     5521 cgcgagttga acgcggagag gcggaaatag ccctcgcccg cggggccgaa acccgagcct
     5581 ggagttccaa cgacctgcgc cttcgatagg agctggtcga agaactccca cgacgtcgcg
     5641 gcgttcttcg tccggatcca gatgtacggc gcgtgcacgc cgccgaagat cgtgaagccc
     5701 gacgccgtga ggccttcgcg caggaggcgc gcgttctcca tatagaaagc gatctgcgcg
     5761 ttcgtctgct tcgagccttc ggcggagtac gccgcggccg cgcccttttg cacgacgtac
     5821 gacgcgccgt tgaacttcgt cgtgtggcga cgagcccaca gtgcattgag cgacacccgc
     5881 tcgcccgcgg gattcgtgcc cgtcaccgtc ttcggaacga ccatgaacgc gcaccgcact
     5941 ccggtgaaac cggcgcgctt cgagaagctg cgcagctcga gagcgacgtc tctggcgcct
     6001 gggatctcgt agatcgagtg cggcagcgaa gaatcggaga tgtatgactc gtacgcggcg
     6061 tcgaacagaa tgatcgagtc gttctgcttc gcccatgcaa cccaacgttc gagctgtgcg
     6121 cgcgtcgcga cggtgcccgt cggattgttc ggcgagcaga gatagacgac gtcgactttc
     6181 tcgctcggcg gatcgggctg gaatccgttg tgctcgttgc cgacgagata gacaaggccc
     6241 ggatagcgac catcacctcc agcagctggc gtgcggcccg ccatcacgtt cgagtcgacg
     6301 tagacgggat acaccggatc ggtcacggcg atttttgcgg cgagcgcaaa gatctcctgc
     6361 acgttgccgc tgtcacactt gctgccgtcg ctcacgaaga tctcgtcggc cgcgatgtcg
     6421 acgccacggg acgcgtagtc gtgctgccga atcgcttcga ccaagaagtc gtagccctgc
     6481 tcggggccgt agccatggaa cgtctcgcgc ttgcccatct cgtcgacggc cgcgtgcatc
     6541 gcttcgacga tcgcgggggc gagcggctcg gtgacgtcac cgatgccgag gcggatcact
     6601 ttcgcatcgg ggttcgcagc ttgatacgcg cgcacccgcc gcccgatctc cggaaacaga
     6661 taggacgcag gcagcttggc gtagtgctcg ttgatacgtg ccatggatgc ggcgaacgta
     6721 tccgagcaac cgtttcgccg ccagcgatcg tctgagcagc gtgaagtggt tgttggtcgt
     6781 cgccctcacc gggctcgtcg ccggttgtgc acacgacgtc gcggcacagt tcccgtcgca
     6841 gccgacggag ccgagcggga cgttggtgct gcagctcacg aggccggcgt cgggtgtgac
     6901 ggtcgcgatc aacggtgttc tcgtcgtcga tgacgagcac acgcagcgcg ttgtcatcgc
     6961 aggtgtgccg agcggaaaca ccgaggtcgt catggcagcg aacggcggcg acaaagcctt
     7021 tcgcgtgtgg atcgacagcg cgcggccgac gaccgtccct ctcggtgttc ccgacgaagg
     7081 ctccggcttt ttgaagtccg tcgccggctc gatcctctcg atcgtcgtgt actcgctcct
     7141 tcaccgatag cgatcggctt acacgcgtgg cacgcgtgat gctgccgacg agagtaggag
     7201 gaagccatga cccgtctgtg gaccacgctg acgctctgcc tcgcggcgac aagccccgcg
     7261 ttcgcgcaga gcgccggtag cgatgacgat tcaaaccaag caggccccgt cgagacgaac
     7321 acgaatgcgg acggactgac ccgtgaaggg aacgttcaag atcgcaccgg gaccgatact
     7381 gacaatccgt ccgattcaac tgctccgacg aacgagcagg tagacacgat ggacccgaac
     7441 aacacgcaga acacatcgag cgacgagacg atgcaaggca cgagtgagca gcctggatac
     7501 gtcgtcgctt cgacgcgcag caccacgatc gagcagagca gcgagcgcga gcgtctcggt
     7561 atcgcgatcg ctgctggcgc cggcgccggt ggtttcacca gcgatgcgct gcgcggctcg
     7621 gcggggctcg gcggcgattg ggacgtgcgg ctgacgctcg gtacgcgttc accgctcgcg
     7681 atcgaagcct cgtacatcgg ttcggcgcaa cggctgagcg cgctcggctt agagaacaac
     7741 gcggtgctcg tcggcaacgg agcgcaaggc gcgctgcgcc tcaacggcac gatcgatttc
     7801 ccggttcagc cgttcatctt cggagggctc gcgtggcggc gttacgacgt gacgaatagt
     7861 gccgcgaata cctcggacgt cgctgaaacc gacgacgtcc tcgaagtgcc agcgggcgca
     7921 ggtctcgccg gcaaggtgtc ggggctcatc gtcgatgtac gcggggaatt ccgatacacg
     7981 atgaacgcgg acctcgtgag aaccggaaat gtcgacacgc ccgtgatcgg agtcagtgac
     8041 aacgaattcg gcacgatgca ccgctggggc atcaacgcga acgtcggata cgagttctga
     8101 tcggaagtcc tccgatggtc gtaagcctcg cgggttgcta ccgtcggggc cttgcgagca
     8161 ttggggctcc tcgcactcgc cgcttgtggc ggaacggccg attcaacaat cgacgtgacg
     8221 catgacccat gcgcgccgct cgccctcgtc agtgctgacg tcacaccgag cgagaccgca
     8281 ggcatgcagg gtgcgcagaa gctctggcgc gagcacggcg cacccgcgct cgggcttcgt
     8341 gcaggcgcga ccctcgaggt tcgctttgac gacgcgtcgc cggcgtttca cggcctctac
     8401 gatgacgaag cgggcgtcat ctacatcaat cggtcgatcc aagacgagac gacgctctcg
     8461 atcgtcatcg cgcacgagct cggtcacgcg tttggcctcg agcacgtcgc tggacgaacg
     8521 tcggtcatga acgccggcaa cctgaaggtc gaaccaaacg acgaggaccg cagcgcgctc
     8581 ggaaacttgt ggggcagctg taactgacga tcagagtgtg tagagaccgc gcgctcccgc
     8641 gagaacgaga gaaaaaaatt tgagctgcaa cgacttagtc cgttgacgag gtcgtcgcga
     8701 ctatggtttt tctagcgacg tgcatagggt gatggtcgtc gacgacgagc ccgacacggt
     8761 cgaggttctg tcgacgttaa tcgagattct tgggtacgag ccacgcgtcg cgaagagcgg
     8821 caaagaagcc atcgagctgg cgtcggtctt cgatccgcac gtcgtgttgc tcgacatcgc
     8881 gttgcccgac atgagcggct acgacgtcgt gcagcagctt cgtgccgaca ccgcgcggcg
     8941 gcgcttcatg gtcgcggcaa ccggctacgc gctggaacgc gatcgcgtac gcgcgctcga
     9001 agcgggcttc gatcaacacc taccgaaacc ggcgacggtt cagaaggtgc gcgaggtcat
     9061 gcagattgct tctcgctacg tctgacgctg gccggcgcga cgagcgcaca ggacgtcgga
     9121 ggcagctcgc ggctatttgc gagacccgct gtcgcggcga tcgtgttggc aacttgcgcg
     9181 cgccgtggct cgtccgcgaa attgcatagc aacgtgaaat cgccgcgatg gatcgcgagc
     9241 acatctccat cgtgctccac gcgcgtcgag ctcggccgag gatcgcgcag cgcgaagtac
     9301 tccttgcgcg cagcaatgag cgactggtac cagccgagca tctgcgcgtg ctccggctcg
     9361 ctgcgctcgt cccagcgaag aacgctcgcg tcgcgagtcg agggctgcgt cggatccggc
     9421 ggcggcttcg accagcccgc cccgccatgc tccgcggccc tgccctcgcg caccgccttt
     9481 cgcagctctt cggactcgag ctgcgcgaag tagaagaacg gcgtcgacgc cgcccactcc
     9541 tcgccttgga acagcatcgg cacgaatggc gacgtcaaca ggatcgccgc tgcgagcgcg
     9601 gtcttctcgt tgcccgcgag gtgatgcagc ctctcgccga gcgcgcgatt gccgacctgg
     9661 tcgtgcgact ggatgtatgc gacgaggcga tcgcgaggga gctggccgaa cggcttccca
     9721 tgacgggtct ggcgaaactt cgagaatcca ccgtcgagcg cgtagccgtc ctcgaggatc
     9781 ttcgcgagcg tgccgggtgc ggcgaagtcc ccgtagtagc cggcgcgctc attcgcgacg
     9841 agcacgtgga tcgcatgatg gaagtcgtcg ttccaatgcg cgtcgaggcc ccagccgcca
     9901 ttggcgcgag gcgcgaccgc gctcggatcg tggtcgtcgt actcgccgat cagcacgatg
     9961 cgacgacgca gctgatgctc gagcgtacga acttcgtcga cgagctctgc gacgaagtgg
    10021 cgctcgctcg tatccttcag cgaatgcaat gcatcgaggc ggaggccatc ggcgccgtag
    10081 tcgcggagcc atgcgagcgc actgtcgatg aagaagcgac gaacttcgtt gctgcccgta
    10141 tcgtcgaggt tgatcgcttc gccccacggc gtcttgtgct tgctcgtcgt gtacggaccg
    10201 aactgcgtcc agtacgcgcc ttcgggaccg aagtggttgt gaacgacgtc gatgatgacg
    10261 ccgagcccgc gcagatgaca ttgccggatc agctcgcgca ggccacgcgg gccaccgtat
    10321 gccgcatgcg gcgcaaactc gtcgacgccg tcgtagcccc aaccatgcgt ccccgagaac
    10381 tgcgccacgg gcatgagctc gacatgcgtg atgccgagct gcacgagatg atcgagatac
    10441 ggcaccgcgc cctcgaacgt gccctcctgc gagaacgtgc cgacgtgcag ctcgtagatc
    10501 accgagtcgc gaagcgccgc tgggtgaaaa tggcctcgcg gtgccggttg cggttcgacc
    10561 caacatgagg cggcgttcac gccgtcgggc tgccatcgcg atctcggatc ggggcacggc
    10621 gccgcaccat ctatcgacag aacgtagcga tcgccctcgg cgagctccgg accttcccac
    10681 cagccgtctg catcttgtcg cgcggcaacg cgggtttccc cgacaacgac gtcaagccgc
    10741 tgtgctcggg gagcccagat ccgaacagcc atgcccgaag gtgatgccag ccgcgtgccg
    10801 ccgacggggt aactatggag acggctgggg taaccgaacc acgaacacgc atcccttcgg
    10861 cgccaggtcg cgcacggtga tgtcgccgcc gtgcgccgcc accgcttcgc gagtgatcgc
    10921 aaggccgagc ccgacgccgc cgcgtcctgg gttggtctgg aagaacgggg tgaagagctc
    10981 gtcgggatta ccggttccga gaccgccgca ctcgtcttcg atctccagga gcgtcgctcc
    11041 ctgcatcgct accgcgcgaa cgtcgacgtg gccgccagga cgcgtgaact tcaccgcgtt
    11101 cagaatcaga ttcgacagag cggactcgag aagtgtcgca tccgcctcga tgtagagcgc
    11161 ctgctccacg aggttgtgaa tcgagacccg gcgatcattg gggattgcct gaatgatgtc
    11221 gtcgagcagc tcgtgcaagt acagcttctg ccggaacagc tgctttcctc cacgcagttg
    11281 aacggtcgcc aatgtctcgg tgatgaggac gtgcaggcgt tgaagacttc gctcgagcac
    11341 acgtgcggtt cggctcgctg tgccaacggt gcctgccttg agcacgttgt acgccatcag
    11401 cgcgctaccg agcgcattgc gtagctcgtg gccgagggat ccgatccaca cggccacgcc
    11461 ctcctcatga ctggcgcgat cgagcgcgaa gtattgctcg agcgactcag cgatcgcctt
    11521 atcgatacac tcgttgagca gcttgtacga acgcggatcg agatagaagt cctgttcgat
    11581 cgcgatctcg ccgatcgtgt cggagatgga accaaagtcc atcgcgatct cgctgatgtc
    11641 gaacccgttg tgataacgct gcgcgccgtg ttccttcgcg tggggaacat gcacggggag
    11701 tggcgtatcc tcggcgaaac cgccctcgcg gcgttccgcc ttgatcagct cgtcgatgaa
    11761 caacggaatc gtatcgacga cctcttcggc aggccgctgc ggatgccgcg cgcgaagctt
    11821 tcgctcgcag cgaatcatga tctcgtctcg aaatcgcgtc agcagctcgt gcaacatgta
    11881 tgagtccccc ctcccttgca ccggcgtcag ccggctcgag actggccaag gatggccgtg
    11941 ctcgcccacg tccgtacgag gacgaggtgc tcctcctcct cggcaatggc gacgcggaac
    12001 tggcgcgcta gatccgcgaa gcccatcgtc tcggcgagct cgacgagcag cgtccagcct
    12061 tcgttgtccg ccgcttcgac cgcaagcatg atgtcgagtg cctgcgtcag cgttgtgcgc
    12121 ggatcgccga gcacctggat ccagccgaga ctcgcgacac ccgcgatgtc cgcacatggc
    12181 gtcatcgctg tcggatccgc gccgagctga accatcgcat cacggacgat cccgaagtgg
    12241 cggcgctcgt catcgcggat gtgctcgatg tcggcgcgcg tcgggccatc gtcgtggacg
    12301 ctcgcggcct cgaacttcgc gagcagcgca tcatagagac gcgcgccggt gcgttcgaag
    12361 gcgaggcgtt cgccgagctt gtcgatcagg acgttcggga cgttcccttc gagcgtctcg
    12421 acgatcgctt tgaccacgcc cttcacggtg ccgggtggtg ggacggtgcc gacgggcatc
    12481 gcctcgcgcg accatcgaac gcgctcgacc tcgagcacgt ggccgtctgc catgccggcc
    12541 gcaggcgtac cttcttcggc gccttggatc gtcttgcgac tgtcgaacgg cgatgtcttc
    12601 acgccggtgc ggtttggacc gatatcggtt ggcttgtgca tggtgtcctc agtggatggt
    12661 catgcgctcg agctcggtgc ccggcgtgta gacgtagtcc gtggaaacga gctcggacgg
    12721 cgagcccgac gagttgagct gttgtcggta ctggagcgac ccctctggct cggcgtcgcg
    12781 cggcacgaac agcgttccgg ccgctcgcag gtcgcgctcg gcatggagtg tcttgcgaat
    12841 gaactcgcgt tggctcgcga actggatcgg atcgggcagt gtcggcggca acagctcctc
    12901 cggatcgcgc ttctcgatct gacgcacgag gtccatcacg tagtgcaggt ggccgagctc
    12961 gtagtcgagc atgcgttccc agatcttttt cagacgtggg ttcggctctt ggccgaggca
    13021 gctccagtac gtgtagacct cgttgcactc gtggagcagc cacttctcga gccagctctc
    13081 ggacggatcg atcaggcact cgtactgcgt cacgtgctgc tcctcgatcg cggcgatctc
    13141 ggcgtacagc attcgcgcga ccggatcggc gaacgacgga ccgatgttca tgtagtagtc
    13201 gtgcacttga tgctccgccg acacgatcgt gagcgcgtgc atcttcgaca gcggcgctgc
    13261 ggtgagcttc gcgtacgagt cgcggatgtc gtcctccggg gcgcggtgct cgtccgcagt
    13321 gggccggccg ggacggacgt ccgtatacga ctgcaggatg ttgttagcgt ctttcccgct
    13381 catgcgatcc atgagcgcgc tgaaccgata caaatgatcg aagtcctcga ggaggccgaa
    13441 ccgatacacc tgcgcgagat acggatcggg ttcctgctgg gccacccatg ccgtgatctc
    13501 gatggccgtc tgttcgaagc cgatcgtcgt ctcgagcggc gactggtcgg cgggattgag
    13561 ccagttgacg agcgtctgct gcaaatgctc gatccggcgc acacgcgcga gcgggccgcg
    13621 cagatcgcga ttcatccgcg cgcaggaatg actgaaccga accgcctccg attcgacgcc
    13681 attcatcagg atcacgcgga cgcgcgtgaa cgcatcgtca tcgagcttgc tgaacggcgg
    13741 gcctgcgagc tcgcgccagg tgaagacctg acgatcgagc gggacgccct tctctttgcg
    13801 aatgtcgagt gccacgactg cctccttgcg gaggcgtgcg ctgcaagcac ggggcccgcg
    13861 cgcacatggc gattcttgct gatgggcgtt gcggagcgtg ccgcacgcgc tcgtgagagc
    13921 gctgcttcga gctagggctg cagttgaacc gcgggttgtt tgccgtcgct gtcgtcggac
    13981 gcgcgttgat acgtccgcgc ttggttcgcg acctcgacgg tcaaactctg tccggtaaac
    14041 gacgtgacct tcatgtcgat cgggaccttg accgcttgcg gattgcattc accgcgggtg
    14101 taaatgacgc gaacgaggcg atcggcgagc gcgacgatcg ttcccttctg ctcgcatcgc
    14161 cccattttgc cgcgatcgat ccagacgtcg attccgcctt tggtatcgat cgtcatctcg
    14221 tacgaccagt ccatgtcgtc ggaggtcacc cagcgaccgg cgaggtctcc aatccgttcg
    14281 acgggcagct ctttcggagg cgcctgccgg gctggggcac cgccacaccc gacgacaaac
    14341 ccgacgacga ccaatagggc gagcctcatg acgagatcgc attatgcggt agagttcgcc
    14401 tccgcatggc gtccgagcgc aacgacttca tccgcgacat catcgacgaa gatctccgta
    14461 cggggcggca cactcgcgtc gcgacgcggt tccccccgga gccgaacggc tatctccaca
    14521 tcgggcacgc gaaagcgatc tgtgtcgatt tcggcgtagc ccgcgactac aacggcacgt
    14581 gcaacctccg ctacgacgac acgaacccga cgaaagaaga tgtcgagtac gtcgagtcga
    14641 tcgagcgcga cgtgcggtgg ctcgggtttg cgccgaccgc cgtgctgttc tcggccgact
    14701 acttccccaa gatgtacgag ctcgcggagc gtctcgtacg cgagggcaag gcatatgtcg
    14761 acgatcttcc cgacgagcag atcaaggagt accgcggcac gctgagcgag cccggcaagc
    14821 cgtcgccgtt tcgcgatcgc tccgtcgacg agaacctgga cctcttgcgg cgtatgaagg
    14881 ccggcgaata tccggacggc gcacgcgtgc tgcgcgcgaa gatcgatctc gcgagcgcga
    14941 acatgaagat gcgcgatccg ctgctctatc gcattcgcca cgcgcaccat caccgcaccg
    15001 gcgacgcgtg gtgcatctac ccgatgtacg actacgcgca tccgctcgag gacgcgatcg
    15061 aaggcatcac gcattcgatc tgcacgctcg agttcgagaa caaccgcgag ctgtacgact
    15121 gggtgatcga caacacgggt ccgtggaatc cgcgcccgag gcagtacgag ttcgcgcgcc
    15181 tcgcgctcga atacacgctg atgagcaagc gcaagctgcg ccagctcgtc gaagaaggcc
    15241 acgtgcgcgg ctgggacgat ccacgtatgc cgacgcttgc agcgctgcgt cgtcgcggct
    15301 acagccccga agcgatccgc gcattctgcg acatgatcgg cgttgcgaag gcgaactcga
    15361 tggtcgacat cgggaagctc gagtactgcg tgcgcgacga tctgaatcag aacgcgccgc
    15421 gcgtcatggg tgtgctgcgg ccgatcgaag tcgagatcgc gggcgatgtc ggcgcgggcg
    15481 ccacagatgc accgtatttc ccgccggacg tcggcaagcc gggtggtcgc gcgctgtcga
    15541 tcagcaatcg catcttcatc gatcgcgatg actggagcga cgatccgccc gccgactaca
    15601 agcggctcgc gccgggccgc acggtgcgtc tgcgccacgg cgtgtgcatc accgccgagg
    15661 aagttgtttc gcgtgatgcc gcgggcgctc cgacgaagat ccgcgcgaag gtgacggatc
    15721 agggcaaggg cgcgggcgtg atccactggg tcgatgcagc gacggccgtg cgcgccgagg
    15781 tccggatcta cgagcgcctg ttcaaggtgc cgaagcccga agaaggtggc ggcgattgga
    15841 tggagcacct cgatcgcgat tcgctgcacg tcgtcgcaga cgcgcgcgtc gaagcgtcac
    15901 tcgcatcggc cgccgtggga tcgcgctggc agctcgagcg cgttggctat ttcgtcgtcg
    15961 acgacgattc gaagccgggc gctctcgtgc tcaaccggat catcacgctg cgcgatgaca
    16021 agcccgcggt cgttgcggcg gcgcccgtcg agaagaagca gaacgtaaag gcggccacgc
    16081 ggccgaagag caagtcaccg acggaatatc gagcggaagc acgcgcacgc gatccagagc
    16141 tagctcgcgc gtatcgcgag gccgaaggct tggggctgcc cgcggagcaa gccgacttgg
    16201 tttcgggcga gcccgccctc gcgcgcctgt tcctcgcggc cgcaacgaag gacaacgcgg
    16261 caatcgtcgc gaagtggatg atcaacgagc tgccgcgcgt cgatgcggag acgaagatcg
    16321 agccggcgcg cttcaaggaa ttcatcgagc tcgtcgcgag tggcgcgctc gcaacggccg
    16381 gcgcgaagtc cgtgctcgcc gatatggtga agaccgggaa gcgagccggg gagctggcgg
    16441 cgagcgctgg acaggcaatc agtgccgacg agctggggac ccgagtccag agtgtgatcg
    16501 aggccaaccc ggacaaggcg gcgcaatacc gctccggcaa gacgggcctc ctcggcttct
    16561 tcgtgggcca ggttatgaaa tccgctccaa atgccgatgc tgccgaggtc aatcgcgtgg
    16621 tccgcgagcg gctcggctag gcacgctggc caggggcgga agtccccaac agttgggaag
    16681 tttcttccca gtcgcaacgc gtacttagct gcgctagttt aacgctcgat gtggaaggtc
    16741 ttggccatgg ctgcgctcct cgccggttgc ggcgcgcggc ttggtggcga cgatcagcag
    16801 ggcgcgacga cggatgcgaa cggttcgaac ggcagcaacg gatcgaacgg cagcaacatg
    16861 cagatggatg catccgtcga tgcaccaccg gcgtgcgcga atggacgcgt cgtgtttctc
    16921 aacttcgagg gcgtcgcgcc gctaacgcag gcagcaacga cggacgcgac cctcaatcgc
    16981 gcgaactggc tgacgaacac gagcgcaacg atcccgccgt atcaccaggg cgttgcgggt
    17041 cgcgcgaccg agatccagac gatcacagat ggtgtgaagt cgcgcctcgc gatgacgccg
    17101 atcaaagttg tcacgactcg tccggcttcc ggaccgtacg tgatggtcgt cttcggcggt
    17161 caacgcaccg cagatggcgg cacggtcggc acgccgtaca cgttggcgac gagcgagcac
    17221 gactgcggcg acaccgcgaa gaacgatgtc ggttgggtct cggacactgc gagcgtctcg
    17281 tatgcgcaag acctcgcgat cggcgccatc ggctggggcc tcggtctcaa cggcacgaac
    17341 gatccgaacg actgcatgtg ctcgtgggcg aacacgtgca actcggcagc gggtgcgtgc
    17401 acgctctccg catcgatcgc gtcgacgacg agcacgggca atacgacgtg cccgaatcag
    17461 aacccgcaaa acgaagtcgc cgcgttctcg acgggtttct gccaatagct ctgtgtcggc
    17521 tcgtgtaggg gagccctcgc ggtagccgtg ggttaagatc gcggccggat gatcggcacg
    17581 accgtcggca gctatcgcgt caccgagaag gtgagcgtcg gcggcatggg tacggtctac
    17641 aaggcggagc acacgctcat cggcaagcac gccgcggtca aggtcctcca ccccgagctg
    17701 cgcaacaatc gcgacgtcgt gcagcgcttc ttcaacgaag ccaaggcgac gagccagatc
    17761 aaccatcccg gcatcgtcga gatgttcgac ttcggctacc tgccgagcgg cgatggttac
    17821 atcgtgatgg agtttctcga gggcatgtcg ctcgcacggc ggctcaagcg cgccggcaag
    17881 atgcccgagg gcgaagcggc gctgatcctg cgcagcgtgt gcagtgcgct cgccgcggcg
    17941 cacgcgaaga acatcgtcca tcgcgatctc aagcccgaca acatcttcct cagccccgat
    18001 ccggaatcgc cgatcggcga gcggcccaaa atcctcgact tcggcatcgc gaagctcaac
    18061 gtcgggaacc cgcgcggcac gcacgcgacg caggcaggcg ccgtcatggg cacgccgacg
    18121 tatatgtcgc cggagcagtg caaaggcacc ggcgacgtcg accagcgcgc cgacctctac
    18181 tcgatcggct gcatcttctt cgagatggtc gcgggccgcc caccgtttac ggagaaggga
    18241 tccggcgagc tgctcggcgc gcatctctat ctcgagccga agacgcccac ccaggtcggc
    18301 gcgaagatct cgcgtgaagc cgaagcgctg atcatgcagc tgctccagaa ggagccgagc
    18361 aagcgcgtgc agagcgcgca agagctcgcg aagaagttcg ccgagctcgc cgcgcatgtc
    18421 gggctcgttg cgcgcgcgac gccgcacccg ctcgtcgagt acgcagccga cgctcacgcg
    18481 acgacgcccg atcacgacgc gcacacgagt gcgctgacga gcgcgcagac aacgcccgcg
    18541 ggctggatga cgccaacgcc gccgggctcc tcgacatcat cgccgacgcc gtccggcgtg
    18601 atgccgaagc cgacgacgct gtcgggtgca gcaagccaag cgtcgatcgc gcgtccccgc
    18661 agctcgcgcg tcgcgatcgc ggtcgcggcg atcgcggtgg cgatggtcgt cggtggcggc
    18721 gttacgatgg cgctcaaagg caaaaagtcg aagaccgcac cggcgccgat cgcgacacag
    18781 ccgacgcaac cagcgcctac gccgccaacc gccgccgcct cggcgcaacc tcccgccgat
    18841 aagtccgcgg ttgtcgagca gcctgcgggt gtcgatacgc gtgcgggtgt cgataagcct
    18901 gcagttgtcg gtacgcctgc ggttgtcgac aagcccgccg tcgtcgataa gcctgcggtc
    18961 gtcgacaagc ccgcggttgt cgataagcct gcggttgtcg acaagcgcgt cgtcacgacg
    19021 aagaagccca agacgaaggc gaaaccgcca accgatgctg gcaagggctc tgggtcggcg
    19081 cagaagatcc tgatcgagac ggatatctga tgcgatttct actcgctgct tttctactgg
    19141 cgcccaccat tgcggtcgcg caaccggcga cgggcgatcg cggcgatgcg aaggcgctca
    19201 tgcagaccgg cgtgaagctg ctcgatgcga aggactacct cggcgcgctg gcgacgttca
    19261 aagaagccta ctcgcggtac ccgagcgcga agatcctgct caacatcggc acgacgctga
    19321 agctgctcgg ccgcgatgcg gaggcggcca acacgtacca gcgctatctc gactcgagcg
    19381 atgcggatgc ggcgcgctcc gaagaagtga agcaggcgct cgtcgagctc gacgccaagc
    19441 tcgggcgcat ctcgatccag acgacgccgc ctgacgccga agtgcaagtc ggcgacgagt
    19501 ggatgccggc cgcgaaagcg aagctcgtgc ggtttgcgcc gggcgacgtg aagctcagcg
    19561 cgcgcaaagc tggcttcgac cccgcgtcga cgacggtgag agcgatcgct ggagccgaga
    19621 cgatggccgc gctgacgctc acggcgacgc tcgtgaaggt cgacgacgcg ggtctcgagc
    19681 aacagcgcat cgaaggagac ctgcgtgcgc acgacgagct gccgatcatc gacgagccct
    19741 ccgtccagcg ctcgcgcgtc ggtgcgctcg cgaacgtcca cgtcgccgtg tatccgaagc
    19801 tcggcagcgc gtggctcgtc ggcggcacct tcgacgcact gccgcgcctc gcgatcgacg
    19861 ctgcgttgct cctcggtccc ggtctcgtca gcgatggtat ggcgacgctg ccgccgccat
    19921 cgttcggcgg ctacgtcggt gcgagctacg cgctgatgct cggcaagctc cgcccgcgtg
    19981 cgcttgtcgg tgtgccggtg tttgcgtcgg acggcgcgcg gctgttcgtg cgcgtcggcg
    20041 gcggagcgga gtacgccgcg actcgccacc tcgcggtgat cgtcgagctc gccggtgaga
    20101 tcgcgctcaa tccgcagaac gacatccgcg acgtcgcgat cgtgcccgcg ctcggcgtga
    20161 ccggccggct ctaatcgacg acgcgcagct tgccgcccga gaacacggga agcgcagctg
    20221 cgtgctgctt cttcggtggt gcttccgcgg cgggacggat gtcgggcgag cgctcggccc
    20281 acgccttcat cacatcgcga tcgaggaggt gtgacggccg cacgttgcgc agcgcgttcg
    20341 ccatgatcga tcgcacgtgc ggattcgttt gctcgagttg cgcgaggagc gccgtcatcg
    20401 catcgcgctt gaggccgtcc tgcgagccgc acaggttgca cgggatgatc ggaaacgcga
    20461 gcgtcgcggc gtactcggcg atatcgcgct cggcgcaatc gatgagcgga cggatgacct
    20521 cgaagcgccc atcgtcggtg cggtagctcg cgggcatcgc ctgcagcttg ccgctgtaga
    20581 acaggttcag caaaaacgtc tcgagcgagt catcgcgatg gtgaccgagc gcgagcttgt
    20641 tgcagccgag gcgctcggcg gccgtataga ggatgccgcg gcgaaggcgc gagcacagcg
    20701 agcagtacgt cgacttgtcg tcaatgtgct cggtgacgac cgagtacgtg tcctcgcgca
    20761 ggatctcgta cggccagccg gtcgcctcga gataaccgcg caggccggtg ccgtcgtagt
    20821 tcggctgcgc ctgatcgagg tgcaccgcca ccatctcgac cttgaacggc aggcgcggga
    20881 tgaggcgcat cagcaggtgg aacagcgtgt agctgtcctt gccgcccgac atcgcgacca
    20941 tgatgcggtc gcccgccgat agcagattgt attcctcaca cgtcgtacgg acggtgcgat
    21001 acaagcgctg ctcctggcgt gcggcctcag ccatgagcgg acttctactc gatcccggga
    21061 ctctcgtcat cctcggaatc gtggcccttg tggctttcac gaccgaaggc gccattggct
    21121 tcggcgggac cgtccttgca gccagtattg gcgcgcagtt cgtgaatctg gaggtcctgc
    21181 tgcccgcatt cgtgccgctc aatattctga tgtcgagctg gctactcggc cgcggcgtga
    21241 aggtcatcgc gtggcgcatg ctgctgaccg aggtcgcacc cgcggtggca gtcgggacgg
    21301 cactcggcct cctgctgttt cacttgcccg caaaaacact gctcgcgctc gtgttcggcg
    21361 tgttcgtcat cggcctcgcg ctgctgcagc tcacgcgtcc cgccgaccgc ccgctcgctc
    21421 gcccactgca gctcggcttc ctcggcctcg gcggtgtcgc gcatggcttg ttcggcacag
    21481 gcggcccgat gatcgtctac gtcgtgcgac ggcgcctgcc cgacaagctc gcgtttcgct
    21541 cgacgctcgc cgtgctgtgg ctcgcgctca acgccgcgct gctcgcgaac tacgcgacga
    21601 gtggcctgta tacgaggaca actgcgcacg tcggcgcggt catcgcgctc gcgctcgtgc
    21661 ccggtttggt cctcggcaac aagctgcatc acaagctcga cgcctcgaag ttcgagcgca
    21721 tcgtgtggat cgtcctcttg ctcgcaggcc tcgcactcgc ggtccgctct gcgttcgcgt
    21781 tgtagtcacg gacagagatg gacgcgactc ggcggctcgt tcatttcgga tacgacgccg
    21841 gtaccgagct ggccatacgc gttccagccc cagcaataga cgtcgccgcc gtcgacgacg
    21901 gcacagtcgt ggtagctccc cgacgtgatc gacaatgcac gtttttgtag cgtgaccgtc
    21961 acgaggtaat cgtcgattcc gggcgtcgag tatccctcac cccagcacac gacccttccg
    22021 ccggcgtcga cggtgcacgt gtgattcacg cccgcactga tcgcggtcac ctgcgagagc
    22081 ggcagcgaga ccgcaccgct cgtcgtcgac gccgtcagca tgccgagctg cgcgaactgg
    22141 ttcgagcccc agcacttcac cgtgccgtcg ctgatgagag cgcacgtgtg ctcgctgccg
    22201 gcggcgaccg ccttcgcgtt cgtcacgccc gccacgagcg tcgggctgac gaggccgggg
    22261 ttggtcgtgg ctccgtctgc ctgcatcttc gcgttcgcgc cccaacacgc gacgtttccg
    22321 tcggtcacgg cgcaggtatg gttgaaaccg gcgccgatcg cggtggcagc cgagatcacc
    22381 gtcgtcggcg tcgtaactgc cagctgcgag ccgttaccga cttcgccgta cgtattcatt
    22441 ccggcgcaca acacgtcgcc gttggcggcc agcgagcaca gatgatgctg tcctccgatc
    22501 agctgctggc tgccggccct atcggcgatc accgtaggcg aaggcagatt cccgatcggt
    22561 ccgccgacct caccaccgtt cgttccccag caatacacgg tcgtgccgtc catcgcgatc
    22621 gcgcaactcg cttggtcggc catgccgagc ttgtcgatcg gtggcatcgc gagcgcctgc
    22681 ggcgttggcg tgcatcggaa ctgcgtaccg ctcgcctgac aactgatcga ccccgtaatg
    22741 gttccgatct cgttctgcgt gttgttgccc cagcacgaga cgccgtggtc atcgcgcacc
    22801 gcgcagctgt ggcctgcgtt cgcggtgacg ctcgacgc
//
LOCUS       DATLBZ010000231         2377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1732231, whole genome shotgun sequence.
ACCESSION   DATLBZ010000231 DATLBZ010000000
VERSION     DATLBZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732231"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VMZ53_12360"
     CDS             complement(<1..783)
                     /locus_tag="VMZ53_12360"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29299.1"
                     /translation="MSSLRYAVRVDAPGTHLAEIELRFTPSGESVDLTLPAWCPGSYL
                     IRDYARFVRDVEVRGDDGRPRVVHKVDKQTWRVARDGARELTVRYGVYGNDLTVRTNH
                     IGDDHAFLHGPATFMFPVDMRREACEVELHFPTGWELTTAAQSVGELSATAAPKKRLS
                     GSRIRTASIDELYDHPIHLGVVRTYALTASVPTKLVIWGQRSPGGQFDEQRLVTDLSA
                     IVDDHIKRLGEAPFTEYTFILMLSHDAYGGLEHRGSSVNLFNP"
     gene            820..1431
                     /locus_tag="VMZ53_12365"
     CDS             820..1431
                     /locus_tag="VMZ53_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29300.1"
                     /translation="MRFAGLLFVALLCASAFAAPRAGKVVRVERRPQQIVGSPRYCTV
                     SPSDNVGYCITTTAPEIGERITVLDNARVLGTIRITTVTPLSDGCQQNSSWMTQGTLE
                     SGDLSNPQGAMIGVLDVAVDLRSGKLINVDKSPSGHQWGTDTVYAIDNNNDGNAEVEF
                     VQYGCDDVGNYSVQSTGLCTEVWSTRSNKGLEKIRSERVKTCY"
     gene            1431..>2377
                     /locus_tag="VMZ53_12370"
     CDS             1431..>2377
                     /locus_tag="VMZ53_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUS29301.1"
                     /translation="MDRRQFLEAERGTLFKEAETRIALVYPSPYRAAMSSLGYQQIYR
                     TLHSMPAVAADRAMLPEDDGKFETLEEGRPVGSYPMLAYSVAYELEIAGVVETLQRAS
                     IPVLRDERDNRHPLIIAGGPLTFSNPAPLAPFCDVIVVGEGEELIVELVTLAREVGFV
                     RERLWDLLAGRPGYYLPHIHGETVPKVAAVDDSLLPARSVIATPHTELADMFMTEAAR
                     GCSRGCTYCVMRRSTNGGMRIVPRADIIAGIPDGTRKVGLVGAAVTDHPDIEGIVADV
                     VSGGRQVGISSLRADKLTDELVGLLAKGGYRTLTVAADGA"
BASE COUNT          402 a          766 c          803 g          406 t
ORIGIN      
        1 cgggttgaag agattcacgc tcgagccgcg atgctcgagg ccgccgtacg catcatgcga
       61 gagcatgagg atgaacgtgt actcggtgaa cggtgcctcg ccgagtcgct tgatgtgatc
      121 gtcgacgatg gccgacagat ccgtgacgag gcgttgctcg tcgaattggc cgcccggcga
      181 tcgctgcccc cagatgacga gcttggtcgg caccgatgcg gtgagcgcgt acgtcctgac
      241 gacgccgaga tggatcggat gatcgtagag ctcgtcgatc gaagcggtgc ggatgcggct
      301 gccggacagg cgcttcttcg gagcggccgt tgccgagagc tcgccgacgg attgcgcggc
      361 ggtggtgagc tcccagcccg ttgggaagtg cagctcgacc tcgcacgcct cgcgccgcat
      421 gtcgaccgga aacatgaacg tcgcgggacc gtgcaagaac gcgtggtcgt cgccgatgtg
      481 gttcgtgcgg acggtgagat cgttgccgta cacgccgtag cggaccgtca gctcgcgggc
      541 gccatcgcgg gctacgcgcc aggtctgttt gtcgaccttg tggaccacgc ggggccggcc
      601 gtcgtcgccg cggacctcca catcgcgcac gaaccgcgcg taatcacgga tcagataact
      661 tcccgggcac caggcgggga gcgtcaaatc gacgctttcg ccgctcgggg tgaatcgcag
      721 ctcgatctcg gcgaggtggg tcccaggcgc gtcgacgcgg accgcgtagc gaagactcga
      781 catacgtggg gcgcagcata gctgctaaac tcgacgctgg tgcgtttcgc cggtctcctc
      841 ttcgtcgccc tcctctgcgc gtccgcgttt gcggcacctc gcgcgggcaa ggtcgtgcgc
      901 gtcgaacgcc ggccgcaaca aatcgtcggt tcgcctcgct actgcacggt ctcgccgtcc
      961 gacaacgtcg ggtattgcat cacgacgacg gcgccggaaa tcggcgagcg catcacggtg
     1021 ctcgataacg cgcgcgtgct cggaacgatt cgcatcacga cggtcacgcc gctgtcggac
     1081 ggctgtcagc agaactcgtc gtggatgacg caaggcacgc tcgagagtgg cgacctgtcg
     1141 aatccgcagg gcgcgatgat cggcgtgctc gatgtcgcgg tcgacctgcg cagcggcaag
     1201 ctgatcaacg tcgacaagtc tccgtcgggt catcagtggg gcaccgacac cgtctacgcg
     1261 atcgataaca acaacgatgg caacgccgaa gtcgagttcg tgcagtacgg ctgcgacgac
     1321 gtcggcaatt actccgtgca atcgaccggg ctgtgcaccg aagtgtggag cacacggtcg
     1381 aacaagggcc tcgagaagat ccggtccgag cgcgtgaaga cctgttactg atggatcgtc
     1441 gtcagttcct cgaagccgag cgcgggactc tgttcaaaga agcagagact cgcatcgcgc
     1501 tcgtgtatcc gagcccgtac cgcgcggcga tgtcatcgct cggctatcag cagatctatc
     1561 gcacgctgca ctcgatgccc gcggtcgccg ccgatcgcgc gatgctgccc gaggacgacg
     1621 gcaagttcga gacgctcgag gaaggccgcc cggtcggcag ctatccgatg ctcgcgtatt
     1681 cggtcgcgta cgagctcgag atcgccgggg tcgtcgagac gctgcagcgc gcaagcattc
     1741 cggtgctgcg tgacgagcgc gacaatcgcc acccgctgat cattgccggt ggaccgctga
     1801 cgttctcgaa ccccgcgcca ctcgcgccgt tctgcgacgt gatcgtcgtc ggtgaaggcg
     1861 aggagctgat cgtcgagctg gtcacgcttg cgcgcgaggt cggcttcgtt cgcgagcggc
     1921 tgtgggacct actcgcgggc cggcctggct actacctgcc gcacatccac ggcgagaccg
     1981 tgccgaaggt cgcggccgtc gacgattcgc tgctccctgc ccgctccgtg atcgcgacgc
     2041 cgcacaccga gctcgccgac atgttcatga ccgaggcggc acgcggttgc tcgcgtggct
     2101 gcacgtactg cgtgatgcgt cgctcgacga acggcggcat gcgcatcgtt ccgcgcgccg
     2161 acatcatcgc ggggattccg gacggcacgc gcaaggtcgg cctcgtcggt gcggccgtga
     2221 cggatcatcc cgacatcgaa ggcattgtcg ccgacgtggt gagcggtggc cggcaagtcg
     2281 gcatctcgag cctgcgcgca gacaagctca ccgacgagct cgtcggcctg ctcgccaaag
     2341 gcggctatcg cacgctgacc gtcgctgccg acggagc
//
LOCUS       DATLBZ010000232        12969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_243026, whole genome shotgun sequence.
ACCESSION   DATLBZ010000232 DATLBZ010000000
VERSION     DATLBZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12969
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243026"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VMZ53_12375"
     CDS             <1..551
                     /locus_tag="VMZ53_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29302.1"
                     /translation="GKTTLANAIREAAIDGVVVPQRFVTRAPRADDVAAEADYVMPAA
                     LDALIARGVVSIYWSRTFEPGHVERYGFESSASGFPVYSANNAICADVRAHAALGNAL
                     FVGVYAADSERGERLRGRSPALFADRPDEARARLEEASDAMRAFVHVIVDNGAGNEAA
                     AQRDIVTLVRNALSVSCSSRLL"
     gene            complement(526..1563)
                     /locus_tag="VMZ53_12380"
     CDS             complement(526..1563)
                     /locus_tag="VMZ53_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29303.1"
                     /translation="MNRAPYLVLVAAVAAGCGGDDSSSGPLGGSLTVTGDVHDFQSGA
                     VLDVTAMVSTTALDPAPTIMTSGAHFTIDGVPENSAFQILATAAPTHHATYSPAIEVT
                     TDDIDGAKAYAVSETFLNAIATGFNVTPSATKGILLLHLVDSQGAAKSGVAASNIVIA
                     NAGSASAPKFLDAQLAAAPAATASSASGWVVVFEVPAGVVELGTAATATATLAMPTSP
                     IAAGAITIAKAVVTDGAPPPLPTNVSFATQVYPIFSARGCIACHSGGGPGKDLGGLML
                     DAGANKVYNELVTETPLRVQKATPEKSLVLTMPSAESPSDGHPNVTFTGPQDADYLKI
                     LAWIKEGAKNN"
     gene            1678..2295
                     /locus_tag="VMZ53_12385"
     CDS             1678..2295
                     /locus_tag="VMZ53_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29304.1"
                     /translation="MRLAASVALVLAALATPARADGFFYGQSYGVSSARSDARPMMGE
                     SLQLRIQMGWRWGNWSVGPWFAGHLAAPREGAKFGVVGGEPAADDSDFESIGADVRYN
                     APIREHLSAYVRGGPRIAQGIGALDGYRGAGLGVGTGVQLTGEVRALGFLFAPLFFAK
                     KGPRIHASLYLDQNVDWYRLSADQMPSLSMPLVGTSIGIGAGSYF"
     gene            complement(2296..2679)
                     /locus_tag="VMZ53_12390"
     CDS             complement(2296..2679)
                     /locus_tag="VMZ53_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29305.1"
                     /translation="MEVTTNNPSASKIDPNVIGEEGASKIKQTFGAARRRIKQIDLRA
                     IIVDNPLAAVGIGLAAGAAVGLIRPMPKRGPVDRLLTGLVTTMVYRAIKQAAFTQLAS
                     YAREYIADHTESKSSARTADVNAPF"
     gene            complement(2690..2947)
                     /locus_tag="VMZ53_12395"
     CDS             complement(2690..2947)
                     /locus_tag="VMZ53_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29306.1"
                     /translation="MQKNGTASALENRFDSLKSSVRDLVDAGGERADQLKSKAIDVKD
                     AVVENGGKYLDRGVTLVKENPFVAIGVAFGVGYLVIRMLRK"
     gene            complement(3209..4357)
                     /locus_tag="VMZ53_12400"
     CDS             complement(3209..4357)
                     /locus_tag="VMZ53_12400"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29307.1"
                     /translation="MTSEPAVELGPDAVVGSYRLMREIGRGGMGTVYEARHVVLPRRA
                     AIKVMHAELRRQPGMATRVVQEASILEDIRHPGVVRVYECALLPDHRPYIAMELVEGE
                     TLASRLAHQGTLSPLEVATLLAELCDVLASVHARGVVHRDLKPDNILITPCDRDFPLR
                     VIDWGVARLGPLGRLTLDGLTPGTPIYMSPEQAMGRNIAAPCDMYSLGVIAYELLTGH
                     PPFDGRTLAEVVSMHLTSAPVPLTEVSNTPPELAELVHRMLDKDLTTRPGAIEVRQIA
                     RGIAFELSTAYESFEVQGAEPPRLPRAVRPRRHMPMTAPPTDDAFVVDPDALEFGITE
                     MVPTIRKPRWTPEIGMAPPPEARPTRAPITPKAASDEIAGEIDARKRR"
     gene            4499..5647
                     /locus_tag="VMZ53_12405"
     CDS             4499..5647
                     /locus_tag="VMZ53_12405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29308.1"
                     /translation="MAGASAKLPPDDRHEGTLRTGSMVSAYRIERLLGTGGMGWVYRA
                     MHVIEAKYVALKVLREDQLRQDRAIDRMMREAQILATVPHPGIPTFFECGLLPDGRPW
                     IAMELIEGTPLSVRIAEGALDHEQVLGILGDVAAVLAAAHQRGVTHRDLKPDNILLTP
                     TDPSHPLRVIDWGIAHHVAGARYTHHDEAIGTPTYMAPEQARGGPSEGHCDVYGLGVV
                     AYQALAGRPPFVGKTSVEILVQHLNKPVPPLAPRCPEAPIGLIELVESMLIKNYCDRP
                     TAEEVLESVATLRSLDDHRAVPTYMTWSDEGTAPRPRIALDDECALELIVPIAAGTPD
                     PDGPKVDFGALENALDAPTTREIPKRPRTHELTPSTTPLRPSLIRSDD"
     gene            5701..5934
                     /locus_tag="VMZ53_12410"
     CDS             5701..5934
                     /locus_tag="VMZ53_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29309.1"
                     /translation="MPKQSQEREYEGNAELERERNQRDSDREHDGGGDELEDDSSQDG
                     ERELERSPMKRGEDRDDIEAELDDLYGMEGPDA"
     gene            complement(5955..6077)
                     /locus_tag="VMZ53_12415"
     CDS             complement(5955..6077)
                     /locus_tag="VMZ53_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29310.1"
                     /translation="MKESDQPRIVQLVLGRMLELVVFSVEGVRGRLARGRNIVF"
     gene            complement(6217..7308)
                     /locus_tag="VMZ53_12420"
     CDS             complement(6217..7308)
                     /locus_tag="VMZ53_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS29311.1"
                     /translation="MSGRLRLLVIDDDLVDRLAIRRAVEQSGLDAQIDEAADADEALL
                     KVGQATYDCLLLDQDLPGMSGIELTRALRAAKRMMPIVFVTGRQAEEVLIEAIDAGVT
                     DFIPKNDLSPRRVGLRVQFAIRIGKAEAESAQAVRSAREAARARDEVLAVVSHDLRGP
                     LHAISLATEALRDEITGPAQRYLGAIERASQRAERLIADLLEASAIENGALTLARAPV
                     DAGSILKQAAADYELLVKETGARINAHIPDDPILVQADRDRVLQVLSNLIGNSLKHAK
                     GAPIELVLERREDAAMILVRDHGPGIPPSELPHVFDRYWSGRTKKGGAGLGLAIAKGI
                     VSAHGGRIEVDSKQGDGAKFLFTLPLARS"
     gene            complement(7337..8830)
                     /locus_tag="VMZ53_12425"
     CDS             complement(7337..8830)
                     /locus_tag="VMZ53_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS29312.1"
                     /translation="MNGTILIVDDDEDTAVLLRDSLRKRGFDVDAVTSGQAALEHLRT
                     SPADVVVTDVQMAGISGIELCQELRQRHPDLLPIVLTGQGGLDTAIAAIRAGAYDFIT
                     KPVKVDALAISVGRAIEHLSLRREVKRLKAASDRDVPIDGIAGNSPAIRETIELVRRV
                     ADSDATVLITGESGTGKELVARALHNLSPRREQPFVAVNCAAMPAPLLESELFGHVRG
                     AFTDAKRSRAGLFVQAGAGTIFLDEIGEMPIEMQVKLLRVLQERKVRPVGGDEEVPFE
                     ARVITATNRDLETEVDEKRFREDLFYRINVVAIPVPPLRARAGDILLLAQYFLKRIAG
                     RTNKPVQGISGPAARLLMDYDWPGNVRELENCMERAVALCRLDEITVDDLPAKIQEHQ
                     SSKIVITTESPGELITLDEMERRYVRQVLNAVGGNKTHAARILGIDRRSLYRRLEEPR
                     TEGQQRKEEPKMSSDHGHPRAPSAPPAHSSAPPPSIPVGGSHPGELE"
     gene            complement(9129..9497)
                     /locus_tag="VMZ53_12430"
     CDS             complement(9129..9497)
                     /locus_tag="VMZ53_12430"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HUS29313.1"
                     /translation="MIGERFRRASTRVATAIGTPFAFIIAFLFVLAWAVTGPLFGFSD
                     TWQLIINTSTTVVTFLMVFIIQTTQNHDAKAIHLKLDELIRAVREARTGLVDLEDLPD
                     DQIDQLQKEFRQLRLQEHTR"
     gene            complement(9494..10600)
                     /locus_tag="VMZ53_12435"
     CDS             complement(9494..10600)
                     /locus_tag="VMZ53_12435"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HUS29314.1"
                     /translation="MTGASEPKTTADAGVEGEKACPAGEHTPRWLHWVTRISIVIGLI
                     ALVITVWIVGPHTLWEQLRKIGWFFIALIALEIVTSICDATAIYFMADGPGRPSWRDT
                     VVAQLAGRGINSITPGGNLGEAVKVGLLAQRCSPRRIVAAVMYVNLVAVVINFAVIAV
                     CSAATAFMFDLPRAAFIGLLVGAAVAAAIAVGIMFLIKRGMLSTLSNALAHMHIISKK
                     RRQSWNNTLEEVDRRLRGQDTAHKSKATLFIAISQIGQKGLTYATVLAAGYMLSPGQF
                     MAYCSAGVLLGWISTIIPMGLGISEGGNVALFSLIGAPASLGLALALARRVNQIAFAI
                     IGFFVLTADRVASRVKGRVKVPLLGKLKPSASHS"
     gene            complement(11057..>12969)
                     /locus_tag="VMZ53_12440"
     CDS             complement(11057..>12969)
                     /locus_tag="VMZ53_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29315.1"
                     /translation="ARARPPDSIPPQFVAPSPPVARRIPLALVGFALAFVGLVLDAGG
                     GPGWGEASARAVLATQQRHLATSPLYDVLANIAALLLPAGEPGFRLGVLAALLGAATL
                     AGITAAVRALVPKEPAAALAPIALLAITPAFREAAAFPTPALLAACGAAWSLAFAIRF
                     ARSSQLAGDSSDARDARDAISALACCAIVVGSAPYLGAVWTVVMLVWLARSGAARSHL
                     AICVGAIGLFVVVLWVGARGELPGIGGASLAATLAATGRGPAAIVVGAGLLGVGFGAL
                     TKMPHARWLACFVGVASLHAAVDGGTGAAGAAGATGAAGAMGAAAGAAVVLVCVLAIG
                     VAILPAAIVRVVANDGSAMRRNAIAALAAVPLVGVALATGAMVRVDDPGGAPTQLARD
                     LENGVPPGPGTFVTTRATSFVVLEYESVVAGRRPDLALVPPLPSREADAIVANALRGD
                     RIAAADAAAFGRLDIRRAIPRRRGFQLVGEAPEKVPAVEGPATYATEIGEQEATLLAV
                     ERARFEAASDRLDGAARAAGLAGTRFHAADLAVLAATTPVPERPAFFGLLPHDPTSPP
                     GPSLLELFGDDLAWVAGLPIAPVAADAPMPRRLHEKWRGILAGTTKLDDPAIAAMGPD
                     AVRATHDVFDAK"
BASE COUNT         2191 a         4195 c         4400 g         2183 t
ORIGIN      
        1 gcggcaagac gacgctcgcg aacgcgatcc gcgaagcagc gatcgatggc gtcgttgttc
       61 cgcagcgatt tgttacgcgc gcgccgcggg ccgacgacgt tgcggcggag gcggactacg
      121 tgatgccggc cgcgctcgac gcgctaatcg ctcgtggcgt cgtgtccatc tactggtcgc
      181 gaacattcga gcccggccac gtcgagcggt atggcttcga gtcttccgcg agcggcttcc
      241 ccgtgtattc ggcgaacaac gcgatttgcg ctgatgtgcg cgcgcatgcg gcgcttggga
      301 atgcgctgtt cgtgggcgtc tatgcagctg atagcgagcg cggtgagcga cttcgcgggc
      361 gatcacctgc gttgttcgcg gaccggcccg atgaggcacg cgcccggctc gaagaagcga
      421 gcgacgcgat gcgcgcgttc gttcacgtca tcgtcgacaa tggcgccggc aacgaagcag
      481 ctgcgcagcg cgacatcgtc actctcgtgc gcaacgcgct tagcgtcagt tgttcttcgc
      541 gccttctttg atccacgcga ggatcttcag atagtcggcg tcttgcggtc cggtaaacgt
      601 gacgtttggg tggccgtccg acggagattc ggcgcttggc atcgtgagca cgaggctttt
      661 ttcgggcgtc gccttctgca cgcgcagcgg cgtttcggtg acgagctcgt tgtagacctt
      721 gttcgcgccg gcatcgagca tgagaccgcc gaggtccttg cctgggccac cgcccgagtg
      781 acacgcgatg cagccgcgtg cgctgaagat cggatagacc tgcgtggcga acgacacgtt
      841 ggtcggcagc ggcggcggcg cgccatcggt gacgacagct ttcgcgatcg tgatcgcgcc
      901 cgcggcgatc ggtgaggtcg gcatcgcgag cgtggcggtg gcggttgcgg ccgtgccgag
      961 ctcgacgaca ccagcgggca cttcgaacac gacgacccag ccggatgcgc tgcttgcagt
     1021 cgcggctgga gcagctgcga gctgcgcgtc gaggaatttg ggggcgctcg cgctgcctgc
     1081 attcgcgatc acgatgttcg acgcagcgac gccggacttc gctgcgcctt ggctgtcgac
     1141 gagatgcaac aacaaaatac ctttcgtcgc cgacggcgtg acgttgaagc ccgtcgcgat
     1201 ggcgttgagg aatgtttcgc tcactgcgta ggctttggcg ccgtcgatgt cgtcggtcgt
     1261 gacttcgatc gcggggctgt acgttgcgtg atgggtgggg gcggccgtcg cgaggatctg
     1321 gaaggctgag ttttcgggca cgccgtcgat cgtgaagtgc gcaccgctcg tcatgatcgt
     1381 gggcgctgga tcgagcgcgg tcgtcgagac catcgcggtc acgtcgagca cggcgcctga
     1441 ttgaaaatcg tgcacgtcac ccgtgacggt gagtgagccg ccgagcggtc cggacgatga
     1501 atcatcgcca ccgcagccag cagcaactgc ggcgacgagg acgaggtagg gcgcgcggtt
     1561 catgacggtc cttcctatcc cagccccact acagtgcgac caggccacac catggtcgca
     1621 gatttttgct atctgcgtga tttttcatgt ggcgcgtcga ttgctgtcta tagatctatg
     1681 cgtcttgcag cgtcggtcgc cctcgtgctg gcagcacttg cgacacccgc tcgcgccgac
     1741 ggcttctttt atgggcagtc gtacggtgtc tcgagcgcgc gcagcgatgc gcgtccaatg
     1801 atgggtgagt cacttcagct gcgaatccag atgggctggc gctggggcaa ctggtccgtc
     1861 gggccgtggt tcgcgggaca cctcgctgcg ccgcgcgaag gcgcgaagtt cggcgtcgtc
     1921 ggtggcgagc ctgcggcgga tgattccgac ttcgaatcga tcggcgcgga cgttcgctac
     1981 aacgcgccga ttcgcgagca tctgtccgcg tacgtgcgcg gtggtccacg catcgcgcag
     2041 ggcatcggtg cactcgacgg ttatcgcggt gcgggtctcg gcgtgggaac cggcgtgcag
     2101 ctcaccggtg aggtgcgtgc actcggcttc ttgttcgcgc cactgttctt cgcgaaaaaa
     2161 ggcccgcgca ttcacgcgag cctctatctc gatcagaacg tcgactggta tcgactgtcc
     2221 gccgatcaga tgccgtcgct ctcgatgccg ctcgttggaa cgagtatcgg catcggagct
     2281 ggcagttact tctgatcaga acggcgcgtt gacgtcggcc gtgcgcgcgc tgctcttgct
     2341 ctcggtgtgg tccgcgatgt actcgcgagc gtacgaggcg agctgcgtga acgcggcctg
     2401 cttgatcgcg cggtagacca tcgtcgtcac gagacccgtg agcagacggt ccaccggacc
     2461 gcgcttcggc atcggacgaa tgaggccgac agccgcaccg gcagcgaggc caatgccgac
     2521 agccgcgagc gggttgtcga cgatgatcgc gcgcagatcg atctgcttga tgcgacgacg
     2581 cgcagcgccg aacgtctgct tgatcttcga cgcgccttct tcgccgatca cgttcggatc
     2641 gatcttcgac gcgctgggat tgttcgtggt gacttccatg gctagctcct tatttcctga
     2701 gcatccggat aacgagatag ccgacgccga acgcgacacc gatcgcgacg aacgggttct
     2761 ctttgacgag cgtgacgccg cgatcgaggt acttgccgcc gttctcgacg accgcgtctt
     2821 tgacgtcgat cgctttgctc ttcagctggt cagcgcgctc gccgccagcg tcgacgaggt
     2881 cccgcacact cgacttgagc gagtcgaaac gattctccag tgccgacgcc gttccgttct
     2941 tctgcatcgt gaattctccc gtgaatggtt gctgctaagt gatcaagacg agcaacacgc
     3001 gtgccccgct cgatgcgttg cagcgcggtg tttgtaggag gcgtcatgcg gagtggccgt
     3061 gcgatgcggt cccgacgtgc gaatagcccg tgcacagagc ctcggcgtcg gcgtttcggg
     3121 gtgtgcgcga gtgctacggc cgagccctca cgctcgccta accgatgcgt tctgccacag
     3181 aaccgccctc ggtcggaccg cggcgcgatc agcgacgctt gcgcgcgtcg atctcgccgg
     3241 cgatctcgtc ggaggctgct ttaggcgtga tgggcgcgcg agtcgggcgt gcctcgggcg
     3301 gaggcgccat tccgatttcc ggcgtccagc gcggcttcct gatggtcgga accatctcgg
     3361 tgatgccgaa ctcgagtgca tccggatcga ccacgaatgc atcgtcggtc ggcggcgccg
     3421 tcatcggcat gtggcggcga ggacgaactg cgcgcggcaa tcgcggcggt tctgcacctt
     3481 ggacctcgaa gctctcgtac gccgtcgaca gctcgaaggc gatgccacgg gcgatttggc
     3541 ggacctcgat cgcaccgggg cgcgtcgtca gatccttgtc gagcatgcga tggacgagct
     3601 ccgcgagctc gggcggcgtg ttcgagactt ccgtcagcgg aaccggcgcg ctcgtcaggt
     3661 gcatcgagac gacttccgcg agcgtgcggc cgtcgaacgg tggatgaccg gtgagcagct
     3721 cgtacgcgat gacgccgagc gagtacatgt cacaaggcgc ggcgatgttg cgacccatcg
     3781 cttgctcggg ggacatgtag atcggcgtgc ctggcgtcaa accgtcgagg gtgagccttc
     3841 cgagggggcc gaggcgagcg acaccccagt cgatcacgcg cagcggaaag tcgcgatcgc
     3901 agggtgtgat caggatgttg tcgggcttga gatcgcgatg cacgacgccg cgtgcatgca
     3961 cggacgcgag cacgtcgcag agctcggcga gcagcgtcgc gacctcgaga ggcgacaggg
     4021 tgccttgatg cgcgaggcgg ctcgcgagtg tctcgccttc gacgagctcc atcgcgatgt
     4081 acgggcgatg gtcgggaagc agagcgcact cgtagacgcg gacgacgccg ggatgccgga
     4141 tgtcttcgag gatcgaggct tcctgcacca cgcgcgttgc catgcctggc tgcctgcgca
     4201 gctcggcgtg catcaccttg atcgcagcgc gacgaggcag cacgacgtgg cgggcttcat
     4261 agacggttcc catcccgccg cgaccgatct cgcgcatgag tcggtaggag ccgaccacgg
     4321 cgtcggggcc cagctcgaca gcgggttcgg aggtcatggc gacccatgct gcacaaagct
     4381 tgcctacaaa ttcgtaagcc aagtagcggt cgaatgtagg cgcgcagggg cggttttgac
     4441 acggcgcgga tcacaccggg ctgtctggca cgcagcgtgc tcggaatgcc gccaatcgat
     4501 ggctggagcc tccgccaagc tacctcccga cgaccggcac gaaggaacac tccggaccgg
     4561 ttcgatggtc tcggcgtatc gcatcgagcg tctgctcggc actggcggta tggggtgggt
     4621 ctatcgcgcg atgcacgtga tcgaagcgaa gtacgtcgcg ctgaaggttc tgcgcgaaga
     4681 tcaactccgc caagatcgcg caattgaccg catgatgcgc gaggcgcaga tcctcgcgac
     4741 ggtcccgcat cccggcatcc cgacgttctt cgaatgcggt ctgcttccgg atggccgccc
     4801 gtggatcgcg atggagctca tcgagggcac accgctctcg gtgcggattg ccgaaggggc
     4861 gctcgatcac gagcaagtgc tcggcatcct cggcgacgtg gcagctgtgc tggcagcagc
     4921 gcaccagcgt ggcgtcacgc accgtgatct caagcccgac aacatcctcc tcacaccgac
     4981 ggatccatcg caccctctgc gcgtgatcga ctggggcatc gcccatcatg tcgccggcgc
     5041 tcgctacacg caccacgacg aagcgatcgg aacgccgacg tacatggctc cggagcaggc
     5101 gcgcggcggt ccgagcgaag gccactgcga tgtgtacggc ctcggcgtcg tcgcatatca
     5161 agcgctcgcg ggtcgcccgc cgttcgtcgg caagacgagc gtcgagattc tcgtccagca
     5221 tctcaacaag cccgtgccgc cactcgcgcc gcggtgtccg gaagctccga tcggtctcat
     5281 cgagctcgtc gagtcgatgc tgatcaagaa ctactgcgac cggccgaccg ccgaggaagt
     5341 tctcgagtct gtcgcgacgc tgcgctcgct cgacgatcac cgcgccgtgc cgacgtacat
     5401 gacgtggtcc gatgaaggca ccgcgcctcg tccgcgcatc gcgctcgacg acgaatgcgc
     5461 tctcgagctg atcgttccga tcgcggcggg cacgccggat ccagacggcc cgaaggtcga
     5521 tttcggcgcg ctcgagaatg cgctcgatgc accgacgacg cgggaaattc cgaagcgtcc
     5581 ccgcacgcac gagctgacgc cgagcacgac gccactcagg ccttcgctga ttcgcagcga
     5641 cgactgatca gatcgcggca tttcgccgcg ggtggcacct ggcttgctcc ttcgctgctc
     5701 atgccgaagc agtcgcaaga gcgcgagtac gaggggaatg ctgagctaga gcgtgagcgc
     5761 aaccagcgag attcggatcg cgagcacgat ggaggcggtg acgagctcga agacgatagc
     5821 tcgcaggacg gcgagcgcga gctcgagcgc agtccgatga agcgcggcga ggaccgagac
     5881 gacatcgagg ccgagctcga tgatctgtac gggatggaag gccccgacgc ttagcggagg
     5941 ccaagaccga cgcgttagaa gacgatgttt cgtcccctgg ccaaccggcc gcggacaccc
     6001 tcgaccgaga acacgacgag ctcgagcatg cgcccgagca cgagctgaac gatgcgtggc
     6061 tggtcggact ctttcatgcg gccgcgatca agcacgcgtc atgccgcgtc aacatgctga
     6121 ttcagccagc agccaagctc agccaatgcg cccccgcagc gcgctgagct gcctcgcacg
     6181 cgctgttgat gcatacgcga ggagcgtgag cggcggctag ctgcgagcga gcggcaacgt
     6241 gaagagaaac ttcgcgccgt cgccctgctt gctgtcgact tcgatgcggc cgccgtgtgc
     6301 ggacacgatg cccttggcga ttgcgagccc gaggccagcg ccgcccttct tcgtgcgtcc
     6361 gctccagtaa cggtcgaaca cgtgcggcag ctccgacgga ggaatgccag gcccgtggtc
     6421 gcggacgagg atcatcgcgg catcctcgcg gcgctcgagg accagctcga tgggcgcgcc
     6481 cttcgcgtgc ttgagcgagt tgccgatgag gttgctgagg acttgcagca cgcggtcgcg
     6541 atccgcctgg accagtatcg gatcgtcggg gatatgcgcg ttgatgcgag cgccggtctc
     6601 tttgacgagc agctcgtagt cggcggcggc ttgcttgagg atcgagccag cgtcgacggg
     6661 cgcacgcgcg agtgtcagcg cgccgttctc gatcgcactc gcttcgagga ggtcggcgat
     6721 gagccgctct gcgcgttgcg atgcacgctc gatcgcgcct aggtagcgct gcgccggccc
     6781 ggtgatctcg tcgcgcaacg cttccgtcgc gaggctgatc gcgtgcagag gtccgcgcag
     6841 atcgtgggac acgaccgcaa gaacctcgtc gcgcgcgcgg gcagcttctc ttgcactgcg
     6901 aacggcctgc gccgactccg cctctgcctt gccgatgcga atcgcaaatt ggactcgcag
     6961 gccgacgcgg cgtggcgaca ggtcgttctt gggaatgaag tccgtaacgc cggcgtcgat
     7021 cgcttcgatc aagacttctt cggcctggcg tcccgtcacg aagacgatcg gcatcatccg
     7081 cttggccgcg cgcaacgctc gcgtcagctc gatgcccgac atgccgggaa gatcttgatc
     7141 gaggagcagg cagtcgtacg tcgcctgccc gaccttcagc agcgcttcgt cagcgtcggc
     7201 agcctcgtcg atctgcgcat cgagcccgct ctgctcgacc gcccggcgaa tggccagacg
     7261 atcgaccaga tcatcgtcga tcacgagtag gcggaggcgg cctgacatag cgtcccttca
     7321 gacgcggacg aacgtactac tcgagctcgc ccggatggct gccgccaacc ggaatcgacg
     7381 gcggaggagc cgaagaatga gccggcgggg cgctcggagc acgggggtga ccgtggtcgc
     7441 tcgacatctt cggctcttcc ttacgctgct gcccttccgt acgcggctct tcgaggcggc
     7501 ggtagagcga gcggcggtcg atgccgagga tgcgagctgc gtgcgtcttg ttgccgccga
     7561 cggcgttgag cacctggcgg acgtaacgac gctccatctc gtcgagcgtg atgagctcgc
     7621 ccgggctctc cgtcgtgatg acgatcttcg agctctggtg ctcttggatc ttcgccggca
     7681 ggtcgtcgac cgtgatctcg tcgaggcggc acagagcgac agcgcgctcc atgcagttct
     7741 cgagctcgcg gacgttgccc ggccagtcgt agtccatcaa caggcgagcc gcgggaccgc
     7801 tgatgccctg cacgggcttg ttggtgcggc ctgcgatgcg cttgaggaag tactgcgcga
     7861 ggagaaggat atcgccagcg cgagcgcgga gcggaggcac cggaatcgcg acgacgttga
     7921 tgcggtagaa gagatcttca cggaagcgct tctcgtcgac ttccgtctcg agatcgcggt
     7981 tcgtcgccgt gatgacgcgt gcctcgaacg gcacttcttc gtcaccgccg acagggcgaa
     8041 ccttgcgctc ctggaggacg cggagcagct tgacctgcat ctcgatcggc atctcgccga
     8101 tttcgtcgag gaagatcgtg ccggcgccgg cctgaacgaa gaggcctgca cggctgcgct
     8161 tcgcgtcggt gaacgcgccg cggacgtggc cgaacagctc gctctcgagg agtggagccg
     8221 gcatcgccgc gcagttcact gcgacgaacg gctgctcgcg gcgaggcgag aggttgtgca
     8281 gtgcacgagc aacgagctct ttacccgtac cggactcacc ggtgatgagc accgtcgcat
     8341 cgctatcagc gacgcggcgg acgagctcga tggtctcgcg aatcgccggg ctgttgcccg
     8401 cgatgccgtc gatcggcaca tcgcgatccg acgctgcctt gaggcgcttg acctcgcggc
     8461 ggagcgacag gtgctcgatg gcgcggccga ccgagatcgc gagagcgtcg accttcaccg
     8521 gcttggtgat gaagtcgtac gcgccggcgc gaatggcggc gatcgcggtg tcgagaccgc
     8581 cctggccggt caagacgatc ggcaggaggt ccggatggcg ctgacgcagc tcttggcaca
     8641 gctcgatgcc cgagatgcca gccatctgga cgtcggtgac aacaacgtcg gcaggagagg
     8701 tgcgcaggtg ttccagcgca gcctgacccg aggtgacggc atcgacatcg aagccgcgct
     8761 tacgcagcga gtcccgcaaa agcactgcgg tgtcttcatc gtcgtcgacg atcaggattg
     8821 ttccattcac ctcggtctct tacctttctg ccgccggaat ctacaagaca gtcgtggggg
     8881 tcgaatcgcg ctcatggcgg catccaggac ctcttacgag gatcgctgga atgaacgcga
     8941 aggggagaaa aatccgtgtc ggagcggcca atgggctgga agccctggga ggttggcgac
     9001 tcgcctcggg ggcatttgcg tataacgtgc cgaaatcgat cggctcgaat ccgcgcagtt
     9061 gcagtgcgca gacgctacaa cgggcgtgcg cccgagcccg tcgagcgcta gagcaccggg
     9121 cgattgcctc acctcgtgtg ctcctgcagc cttagctgac ggaattcttt ctgtagctgg
     9181 tcgatctggt cgtcgggcag gtcctcgagg tcgacgaggc ctgtacgtgc ttcacgcacc
     9241 gcccggatga gctcgtcgag cttgaggtgg atcgccttgg cgtcgtggtt ctgggtcgtc
     9301 tggatgatga agaccatcag gaaggtcacg acggtcgtcg acgtgttgat gatgagctgc
     9361 caggtgtccg agaacccgaa cagcggtccg gtcaccgccc aggcgagcac gaacaaaaac
     9421 gcgatgatga aagcgaatgg ggtcccgatg gccgttgcca cgcgggtcga cgctctgcgg
     9481 aaccgttcgc cgatcatgag tgggatgcgc tcggcttgag cttgccgagg agcggcacct
     9541 tcacgcggcc tttaacgcgc gaggccactc ggtcagcggt gagcacgaag aagccgatga
     9601 tggcgaaggc gatctgattc acgcgacgcg cgagcgccag cgcgaggccg agcgacgccg
     9661 gtgcaccgat gagcgaaaac agcgcgacgt tgccgccttc gctgatgccg aggcccatcg
     9721 ggatgatcgt cgagatccag ccgagcaaga cgccggcgga gcagtacgcc atgaactgtc
     9781 cgggcgagag catgtagccc gctgcgagga cggtcgcgta ggtcaggccc ttctggccga
     9841 tctgcgagat cgcgatgaac agcgtcgcct tgctcttgtg cgcggtgtcc tgaccgcgga
     9901 gccggcgatc gacttcctcg agggtgttgt tccagctctg gcggcgcttc ttcgagatga
     9961 tgtgcatgtg cgcgagcgcg ttcgacagcg tcgagagcat gccgcgcttg atgaggaaca
    10021 tgatgccgac ggcaatcgcg gcggcgacgg cggcgccgac gagcaatccg atgaacgctg
    10081 cccgcggcag atcgaacatg aatgcggtcg cggcgctgca caccgcgatc accgcgaagt
    10141 tgatgaccac ggcgacgagg tttacgtaca tgaccgcggc gacgatgcgg cggggcgagc
    10201 agcgctgcgc gagcaagccg actttgacgg cctcgccgag gttgccgccg ggagtgatcg
    10261 aattgatgcc gcgtccggcg agctgagcga cgacggtgtc acgccacgac gggcgtcctg
    10321 gcccatcggc catgaagtag atcgcagtcg cgtcgcagat cgacgtgacg atctcgagcg
    10381 cgatgagcgc gatgaagaac cagccgatct ttcggagctg ctcccagagc gtgtgcggtc
    10441 cgacgatcca aacggtgatg acgagcgcga tcaacccgat cacgatcgag atgcgcgtga
    10501 cccaatgaag ccagcgcggc gtgtgctcgc cggcggggca ggccttttca ccctcgacgc
    10561 ccgcgtctgc ggtcgtcttc ggttcgctcg ctcccgtcac tcgcgcgctc tccacgtaga
    10621 aacgtgctcg gctgcctcac cgcagcaaga ttcaccgagc gccagcacct cgcctcgacg
    10681 aggaccggaa gtgccaagcg gggtcatgtg tgtagattct gcagtcgggg tgccaatggg
    10741 aactcctgga aactcctgaa agatgccctg cgggctgcgc gaatacgccc gctgtgtcga
    10801 aaacgcgcag tctgcaacct tcggaagtat cggattcccg atctgtttgc gggcatctag
    10861 ccgcgaaacg tcgcttggta tcgagcgtgc aaaggtgctc atccgtcgcc gggcagcctc
    10921 ggcgcgaccc cacatctcga tcggcgtcct ccggttgcgt tccacccgcg cagccaggtc
    10981 gccatgaccc ccgagccccc cagctcgggt cccgccggtg cacctccccc aggtcatcgg
    11041 cggttttttt ttcggcctac ttcgcgtcga acacgtcgtg cgtggcgcga accgcgtcgg
    11101 ggcccatcgc ggcaattgcc ggatcgtcga gcttcgtcgt gcctgcgaga atcccgcgcc
    11161 acttctcgtg cagacgacgc ggcatcggcg catccgcagc caccggcgca atcgggagtc
    11221 ccgcgaccca cgcgaggtca tcgccgaaga gctcgagcag cgatggtcca ggcgggctcg
    11281 tcgggtcgtg cggcagtaag ccaaagaatg ctggtcgctc gggcacgggc gtcgtcgccg
    11341 cgagcacggc caaatccgcc gcatgaaagc gcgtgccggc caaccccgca gcccgcgccg
    11401 ctccatcgag ccgatcgctc gcggcttcga agcgcgcgcg ctcgaccgcc agcaacgtcg
    11461 cctcctgctc gccgatctct gtcgcgtacg tcgccggtcc ctcgacggcc ggcaccttct
    11521 ccggcgcttc gccgacgagc tgaaagccgc gtcgacgcgg aatcgcgcga cgaatgtcga
    11581 ggcggccaaa cgccgcggcg tccgctgccg cgatgcgatc gcctcgcaac gcattggcga
    11641 cgatcgcgtc ggcttccctc gacggcagag gcggcacgag cgcgaggtcc ggccgcctac
    11701 ccgcgacgac gctctcgtac tcgagcacga caaagctcgt cgcgcgcgtc gtcacgaacg
    11761 tgcccggtcc aggcgggacg ccattctcga gatcgcgcgc gagctgcgtc ggcgcgccac
    11821 ctggatcgtc gacgcgcacc atcgccccag tcgctagcgc aacgccgacg agcggcaccg
    11881 ccgcaagcgc ggcgatcgcg ttgcgccgca tggccgagcc atcattcgcg acgacgcgca
    11941 cgatcgcagc cggcagaatc gcgacgccga ttgcgagcac gcagacgagc acgaccgccg
    12001 ctcccgccgc tgcgcccatc gcgcccgccg cgcccgtcgc gcccgccgct cccgccgcgc
    12061 ccgtcccgcc gtcgacggcc gcatgcagcg acgcgacgcc gacgaaacac gccagccacc
    12121 gcgcatgcgg catcttcgtg agcgcgccga acccgacgcc gagcaacccc gcgccgacga
    12181 cgatcgcggc cggccctctg cccgtcgctg ccaacgtcgc ggccaagctc gcgccaccga
    12241 tgccaggaag ctcgccgcgc gcgccgaccc acaacacgac gacaaacagc ccgatcgctc
    12301 cgacgcagat cgcgagatga gagcgcgccg cgcccgaccg cgccaaccac acgagcatca
    12361 cgaccgtcca caccgcgccc aaatacggcg cgcttccgac gacgatcgcg cagcacgcga
    12421 gcgcgctgat cgcatcgcgc gcatcccgcg cgtccgacga atcgccggcg agctgcgatg
    12481 agcgcgcgaa gcggattgcg aaggcgagtg accacgctgc gccgcaagcg gcgagcaatg
    12541 cgggagtcgg aaacgcggcc gcttcgcgga atgcaggcgt gatcgcgagc agcgcgatcg
    12601 gcgcgagcgc ggcggccggc tccttgggga cgagggcgcg caccgcggcg gtgatgccgg
    12661 cgagcgtagc cgcgccgagc agggcggcga gcacgccgag gcggaagccg ggctcacccg
    12721 cgggcaggag cagcgcggcg atattcgcga gcacgtcgta gagcggcgat gtggcgaggt
    12781 ggcgctgctg cgtggccagg accgcgcggg ccgacgcctc gccccagccg ggaccgccgc
    12841 ccgcgtcgag gacgagcccg acgaatgcga gcgcgaaccc gacgagggcg agcgggatcc
    12901 ggcgagccac gggcggcgac ggtgccacaa actgtggcgg aattgagtcc ggtggacgcg
    12961 cacgagcac
//
LOCUS       DATLBZ010000233         2192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9183528, whole genome shotgun sequence.
ACCESSION   DATLBZ010000233 DATLBZ010000000
VERSION     DATLBZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9183528"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VMZ53_12445"
     CDS             complement(<1..680)
                     /locus_tag="VMZ53_12445"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29316.1"
                     /translation="MTERFERLLTLRGDAVKGLPVSAWEANLNDVDRDQLLRAVIDVI
                     RKLLLPDWEVGHKDDRRPQAALEAAEKWIETKSADAATEAKTAAKACTAARNETFGKD
                     HRAPEAARAVAWAAGAKDNSNIWEALTAIEGELLARITLVGEYHRQPEMRRAIVDALR
                     NALTPKQAATESATSSDPVPYAASGNFAVGQKLVHAKFGNMVVAAAGDKWIDVDLEDG
                     TRKRLAQKP"
     gene            708..1217
                     /locus_tag="VMZ53_12450"
     CDS             708..1217
                     /locus_tag="VMZ53_12450"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29317.1"
                     /translation="MKRTLDAKRLTETEIRCISRWVAACAARVLPVFEAAAPNDSRPR
                     DAIEGARAFARGGKRTATLRSLALAALAAAREVRDPAAMAAARAAGYAASSAFTHPGM
                     EKHQIKHVLGPVVYAVLAKDDREISWALRRATPALRNIVRRMQPHRVSRSRLDQLYAR
                     LDTGLRHRG"
     gene            1225..1560
                     /locus_tag="VMZ53_12455"
     CDS             1225..1560
                     /locus_tag="VMZ53_12455"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS29318.1"
                     /translation="MSRERRQHERFELLAQIELSRGTKIETFVAINISAGGVLLRNDD
                     NVEFSVGETVRIQFDVPELAAKFAIDATVIRTIAPTARPAAIAAMWTSSDAPATAALG
                     EMLWRLKGT"
     gene            1561..1944
                     /locus_tag="VMZ53_12460"
     CDS             1561..1944
                     /locus_tag="VMZ53_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29319.1"
                     /translation="MPVTIDRVNAHAMKFPDVSVGTKWGNRTWMINNKGFVWQRPLRA
                     SDLKRLGDEVPPSDPIIAIRTENLEAKDGLLAMDLPGFFTIQHFNGYPAVLVELKKAR
                     AADVRMAIELAYRAVATRRPRRRSR"
     gene            complement(1965..>2192)
                     /locus_tag="VMZ53_12465"
     CDS             complement(1965..>2192)
                     /locus_tag="VMZ53_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29320.1"
                     /translation="QNVSRISCWILPFEALYQSALGRLTTDTFGITRIAIDLGPFGGA
                     QNFGSLMWPWTMAYLLIIGGLALRSFARRDL"
BASE COUNT          371 a          705 c          728 g          388 t
ORIGIN      
        1 ggcttctgcg cgagacgctt gcgcgtgccg tcttcgagat cgacgtcgat ccacttatcg
       61 cctgcagcgg cgacgaccat gttgccgaac ttcgcatgca cgagcttctg gcccactgca
      121 aagttgccgc tcgcggcgta cggcaccgga tcgctcgacg ttgccgattc cgttgcggct
      181 tgcttgggtg tgagcgcgtt gcggagcgca tcgacgatcg cgcggcgcat ctcgggctgg
      241 cggtggtatt cgccgaccag cgtgatccgt gcgagcagct caccctcgat ggcggtcagc
      301 gcttcccaga tgttgctgtt gtctttcgcg cctgccgccc acgcaaccgc gcgtgcagct
      361 tcgggcgcgc gatggtcttt gccgaacgtc tcgttgcgcg cagcggtgca cgccttcgcg
      421 gcggtcttcg cctctgtcgc ggcgtctgcc gacttcgtct cgatccactt ctccgccgct
      481 tcgagcgcgg cttgcggccg gcgatcgtcc ttgtgcccga cttcccaatc ggggagcagc
      541 agcttacgga tcacgtcgat cacggcgcgt aggagttgat cgcggtcgac gtcattgagg
      601 tttgcttccc acgcggaaac cggaagacct ttgactgcat cgccgcggag agttaacagg
      661 cgttcgaagc gctccgtcat cgcggctcga ctataagctg cattgcaatg aagcgaacgc
      721 tcgatgcgaa gcgcctcacc gaaaccgaga tccggtgcat ttctcggtgg gttgcggcgt
      781 gcgcggcgcg cgttttgcct gtattcgagg ccgcggcgcc gaacgacagc cgccctcgcg
      841 atgcgatcga gggagcccga gcgtttgcgc gcggtggcaa gcggacggcg acgctgcggt
      901 cactcgcgct ggcagcactc gcggcagcac gtgaggttcg cgatccggcg gcgatggcag
      961 cggcacgcgc agcgggctac gcggcatcga gcgcgttcac gcatcccggt atggagaagc
     1021 atcagatcaa gcacgtgctc ggcccggtcg tgtacgcggt gctcgcaaag gacgaccgcg
     1081 agatcagctg ggcgctgcgg cgagccaccc cagcgctgcg gaatatcgtt cggcgcatgc
     1141 agcctcatcg ggtcagccgg agccgcctcg atcagctgta cgcgcgtctc gacaccgggc
     1201 tgcggcatcg cggatgatcc gtggatgtct cgcgaacgtc gccagcacga acgcttcgag
     1261 ctgctcgcgc agatcgagct gagccgcggc acgaagatcg agaccttcgt cgcgatcaat
     1321 atctcggccg gcggcgtgct gctgcgaaac gacgataacg tcgagttctc cgtcggcgag
     1381 acggtccgca tccagttcga cgttcccgag ctcgccgcga agttcgcgat cgacgcgacg
     1441 gtcatccgca cgattgcacc gaccgcgagg cccgccgcga tcgctgcgat gtggacctcg
     1501 agcgacgcac cggcgaccgc agcgctcggc gaaatgttgt ggcgcctgaa gggaacctag
     1561 gtgccggtca cgatcgaccg tgtgaacgcg cacgcgatga agtttcccga cgtcagcgtg
     1621 gggacgaagt ggggcaaccg gacgtggatg atcaataaca agggcttcgt gtggcagcgg
     1681 ccgctgcgcg catctgatct gaagcgcctc ggcgatgaag tgccgccctc cgatccgatc
     1741 atcgccattc gcaccgagaa tctcgaagcg aaggacggtc tcctcgcgat ggaccttcca
     1801 ggcttcttca cgatccaaca cttcaacggc tatcccgcgg tgctggtcga gctgaagaag
     1861 gcgcgcgcgg ccgacgtgcg catggcgatc gagctggcgt atcgcgcggt ggcgactcgc
     1921 cgaccccgac ggcgctcacg ctgatgagct gcaagtcgtc gtgattacag gtctcgacgt
     1981 gcaaatgacc tgagcgcgag gccgccgatg atgagcagat aggccatcgt ccagggccac
     2041 atcagcgacc cgaaattctg ggccccgccg aacggaccga ggtcgatcgc gatgcgcgtg
     2101 atgccgaacg tatcggtcgt gagccggccg agcgcggact gatagagcgc ctcgaacggc
     2161 aggatccagc acgagatgcg cgacacattc tg
//
LOCUS       DATLBZ010000234        23077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10557887, whole genome shotgun sequence.
ACCESSION   DATLBZ010000234 DATLBZ010000000
VERSION     DATLBZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 23077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23077
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10557887"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VMZ53_12470"
     CDS             complement(<1..408)
                     /locus_tag="VMZ53_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29321.1"
                     /translation="MRRMLADRPGVLRYVLFAIAVTGVAASTRCADDRGTSSPRTVDG
                     QRASIHIKFREAHVHALGLGEQPLFAKHYGSYAGAWGPPRQDPRDRWMRVDVPASEAA
                     SVVAQLEQDVAIEQAFVAPEITLPRSERVRDDDE"
     gene            complement(418..972)
                     /locus_tag="VMZ53_12475"
     CDS             complement(418..972)
                     /locus_tag="VMZ53_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HUS29322.1"
                     /translation="MYSPDRYVAAMRFAAERHLAQKEPGSELPYLVHIVSVAAEVIAA
                     LPTTTLADPDLAVTCALLHDTVEDTATTLDEVTAKFGAAVAAGVSALSKNDALLVDGR
                     TATKREKMLDSLRRIREQPTEIAVVKLADRITNLSEPPHYWPPEKRAAYREEGLLIAD
                     TLGYASATLDARLRARAEAYKAFC"
     gene            complement(982..1683)
                     /locus_tag="VMZ53_12480"
     CDS             complement(982..1683)
                     /locus_tag="VMZ53_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS29323.1"
                     /translation="MRPIRQRRADRREEREERREARRRRTPEVARLELLDAAERLFNV
                     ERPDDVGLKDVAKEAGTSHALITHYFGTYAGLVEAVLQRRLLRLRETVATRLGSAGAL
                     ERPDEMLAQLFSTLGDPVHVRLMKWVMASGRGESMQVFALQQQGLQAVAHQVASAFLA
                     MPGCPPVPETKRRELVEKIELSLVTAVAAALGYALTKDALSGAIGRQQTPELDTAVQR
                     TLGAMLQAYMRELIG"
     gene            1795..2553
                     /locus_tag="VMZ53_12485"
     CDS             1795..2553
                     /locus_tag="VMZ53_12485"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HUS29324.1"
                     /translation="MNSSSTTPPRSTDRSHIVDVADLPPHIRWARALRALGRILRNPE
                     ETDQVLVFSNLANAGRRDPDELNRLFTDPQAARLFREQRALDSKTIDVDVLAALPEGT
                     LGRAYATFMKAHGLTPNVFDGSPDDVRDERAAYLIQRMRQTHDLWHVVTNCETDPAGE
                     IALQAFTYAQVRAPSSAILALLGTLKSLRYTRDVAPDALELYRAGAAAKPLVFFPWED
                     HWATPLVDVRRLLGLPANPRKVGGYLTEAMTLAA"
     gene            2800..3111
                     /locus_tag="VMZ53_12490"
     CDS             2800..3111
                     /locus_tag="VMZ53_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29325.1"
                     /translation="MGSRNGDGVRAKRRRLRVPAALAVALVGSSMSIAASVVGCSPSE
                     EPPVPDAQPGGTIGGTRQDASVSGDGAGSEDAGLPPDAPSVTDAALDAPLEPTPDAPH
                     T"
     gene            3213..3410
                     /locus_tag="VMZ53_12495"
     CDS             3213..3410
                     /locus_tag="VMZ53_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29326.1"
                     /translation="MKRPRLRIPAVLAVAVVGTSATVTLTFSGCGAEPGPEPIDANRI
                     NSVKVDAGVDAVDAPDARPPI"
     gene            3457..5004
                     /locus_tag="VMZ53_12500"
     CDS             3457..5004
                     /locus_tag="VMZ53_12500"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HUS29327.1"
                     /translation="MSEQRWIRSQRAAVVDAPSRGSGSDGDGDGLIVVGRDARVFKLE
                     GDSAQLARVVVAFLGRPHSEREVLAHLEALGGEPLGDRTTVVQQLLTMLAETGAISSA
                     PADTPTTAANANEPPPKVRPAGLNVVVGITGAIAASSAPGLVTALQRRGHTVEICVTP
                     TAQRFVAIDALTAIAQREVHTSMWPRTAHAPVPHVALAQWADLVIVYPASGTTIARIA
                     HGEFSELVAAVALTTRAPVVLVPSMNAEMLAAAPVQRNLDQLREDGFAIVHGVPSHEV
                     ADAPSVRDSIAGAAPAPGEVVATIEALRAAAVLERRTDDAPGPRAWEAVYRKASASEP
                     AANLLPWASSECDADIVQALAEHAPRGRLLDVGCGLGQVARHAARSNYRVVATDVSDS
                     ALALARDVTPDDDIIWLRDDICASGLAGTFDVIVDRASLHTFSPLRAHAWAATLARIT
                     RAGSTVIVKAHKAGVPPATRGYSAQSIAALLPDFAVIAERDAELPGLVDANPIASTLV
                     VLRRRGR"
     gene            complement(5001..5213)
                     /locus_tag="VMZ53_12505"
     CDS             complement(5001..5213)
                     /locus_tag="VMZ53_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29328.1"
                     /translation="MPVAGRPGGTYISNEAIPVLHPGRMMRAPSSTNSARARFDAAMG
                     ATAPVGIGKRIVRPSALIAAAMSLSA"
     gene            5366..6355
                     /locus_tag="VMZ53_12510"
     CDS             5366..6355
                     /locus_tag="VMZ53_12510"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS29329.1"
                     /translation="MSRVAITGASGLLGGNLAAELVAAGHQVVATRRAGTKVRHLDDL
                     AIDWRDGDLANAGSLTRAFENCDAVFHCAAAVGVEKIVTKEMRDTNVGGTERVIDACV
                     AAGVKRLVHTSSVVAIGLTTSGVPSDETATWNFDKEGLVDAYAITKHEAEEVVRKAAA
                     ATLDAVIVNPTYMFGPRDARPSSGTLILKIAKRRVPGWTPGYNNFVDVRDVARGMIAA
                     WHKGRRGERYILGGHDMTYRACFETIAKVARVKPPRLGVPYPLAKLLGRFGDILEARG
                     REPLVNSTQIEYAFTDKFRFHSTKAASELGYTYGPLEPAIREALDWFRANGML"
     gene            complement(6352..7479)
                     /locus_tag="VMZ53_12515"
     CDS             complement(6352..7479)
                     /locus_tag="VMZ53_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HUS29330.1"
                     /translation="MSSEGDARTKLDPLRQELGQIDREILALVSKRQAVAQKIGQVKR
                     DGGIPTRDYRQERDVVERARAAAVQHGLTPQLGEELILALIRGSLTVQEKDTVAASGE
                     GSGRRALVIGGSGHMGRWFVRYLAAQGFTVESADPTEPPVRHANIAYHRDWKTLALDH
                     ELIVIAAPMPATNEILEEMAKQPPKGVVFDVGSLKSPLRKGLTALRAAGAQVTSVHPM
                     FGPDTELLSGRHVIFIDLGAPQATAAARALFEPTMATLVEMDLESHDRLIAYVLGLSH
                     ALNIAFFTALAESGEAAPKLAQLSSTTFDAQLGVAAKVAAENPDLYFEIQTLNDYGTE
                     SLAALMYAVERLRSVVRAGDIEGFRGLMTRGQKYLTTRQPR"
     gene            complement(7503..8123)
                     /locus_tag="VMZ53_12520"
     CDS             complement(7503..8123)
                     /locus_tag="VMZ53_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HUS29331.1"
                     /translation="MEELRILARLNEETQVHHADADSDVDRYLFCSNVSSADYRTYLQ
                     RVYGFLVPLEAALATAPDLDTVIDAKGRAKSALVAHDLFALGMTMDEVSALPHCTRIP
                     AFRGPAAALGWMYIAERPLLASAVIRGHLASYLPTEMATASAYLMCYAGQVGAMWREL
                     GLAMQQVAGSPAVADRIVVAAHDAFRTLHRWRTQELGRASVIRYAG"
     gene            complement(8276..9301)
                     /locus_tag="VMZ53_12525"
     CDS             complement(8276..9301)
                     /locus_tag="VMZ53_12525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS29332.1"
                     /translation="MLSRIDRLAYHSQHLTFLVNATMMEETARLISRNPRPHVPFAAW
                     KTLRERRGELHRIDLANVEAGYYPRELLFDVPVRRYLREFPRLVLDAPKVVRRMRKNA
                     FKDIPDVDKERFPPYYRRNFHWQTDGYFSDFSAQVYELGVELLFRGTADVMRRQVIPP
                     ITELVRATEGGAAKMKLLDIGCGTGRTLHQIARTHPQMRLHGCDLSPNYIKLARKRLE
                     DVAELTLAVENGEALPWADNTFDVVTSTYMFHELPRNARRNVIREMLRVVRPGGLCVL
                     EDSAQASESPELAEVLLGFPQEFHEPFYADYLRDDLAAILDEIGFDVRETQARLVSKI
                     VVANKPV"
     gene            complement(9311..10768)
                     /locus_tag="VMZ53_12530"
     CDS             complement(9311..10768)
                     /locus_tag="VMZ53_12530"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HUS29333.1"
                     /translation="MRKALAVLFLTALASAAQANGRDPYTVTIHFKRGAENNIISGMT
                     FGLVISHDGGTTWEWMCEKAIGYGGMWDPDYEYTSSDAIFATTFDGLSVMRNRCDFAS
                     TMFGTTFVSRITEASNGTLYVAASDSTIGDSKIYKSTDNGMTFPTSTTPPGGIAGDWW
                     QSIEVAPSDPTRVYLTGYRLVMKCTANSGNAGAACTANAMCTNGGMCEPQKEFLLYKS
                     VDSGANYTAISKTGIGTTSSNSTIDIVGISPTDPNTLYVKVTFETGSSGDTVYRSTNG
                     GTNWTPILSKDSKFGLSFLVRYDGTCVAGTRELGAWKSADCATAATPTWTPLTTAPHI
                     GCLYENSAHEVWACTQMMASVQLNIDADGYGIMKSTDLSTWTGVLKYPDIKAPVACAV
                     GTVQNDQCVERFEGMQSPWCCLVPQLGITSTEVDCTGARGCFNDTIDGAIDGIQKPEK
                     PGCCEAGSSGSTAMLSGLVVAGLVFRRRRRPTARR"
     gene            complement(10797..11732)
                     /locus_tag="VMZ53_12535"
     CDS             complement(10797..11732)
                     /locus_tag="VMZ53_12535"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HUS29334.1"
                     /translation="MASQNIPVVDLGDWHEGGAARQRFVTTVGESLADIGFFAVRNHG
                     VPDTLLARSYEVAKAFFHQPNEAKQRYHDPKLKGQRGFTGMGREHARDSEAADLKEFW
                     QVGRPGMNHPQYGDNVWPAEVPDFGPTMTDLYQRLDSLGTNLLRACAAYIGEASERFA
                     ELAQDSDTIVRVLYYPPVGSKVPPGAVRSAAHEDINLITLLSGATAEGLELLRRDGSW
                     MPVHTGFDTIVVDSGDMLQNLTNGLYKSTTHRVVNPPDATSERYSIPCFVHARSECDL
                     TPLPSCVARTGRVAKYPSVTAGAYLEQRLREIGLA"
     gene            complement(11744..12334)
                     /locus_tag="VMZ53_12540"
     CDS             complement(11744..12334)
                     /locus_tag="VMZ53_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS29335.1"
                     /translation="MKWLIALVALAGCDKLFSVTHVPLHVDSGADAPLEACPTVDYVA
                     VGGTAQTSKYRYVSPKGLPWDAAELDCETDSLTKITHLVVFDDPSEMAAVRSLVPASP
                     PYWQVFTGYARNADAQGGIPFSFTSVTGTPIEASSPIWEKVNGVEPDNGGTMFPEETI
                     TFFEMTRNLTDGPASMQLGYICECDGKVANQTFKIR"
     gene            complement(12331..12627)
                     /locus_tag="VMZ53_12545"
     CDS             complement(12331..12627)
                     /locus_tag="VMZ53_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004900862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HUS29336.1"
                     /translation="MWVVYLVRCADATLYCGITNDLEARIAAHSVGKGAKYTRRRGPV
                     TLVFSQRCRTKHLALRIEYAIKQLTRPEKDALVAEPTRIAPIVVRARRQLRAAA"
     gene            complement(12630..14321)
                     /locus_tag="VMZ53_12550"
     CDS             complement(12630..14321)
                     /locus_tag="VMZ53_12550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUS29337.1"
                     /translation="MLTFSTNPDVAEQQMNAIIFYMTAFGYIDGDFDFTEKTFVRIYI
                     RQLVTARANTALPDADAKTKDEVINKFTTHFHEVFEQIDRGVKGLFDEAVADGEDVEK
                     FVYSKLKLRSYEIFQSFDRDNQHELLATIDELIYADGSVHPAEKKFREEIEALLDTGA
                     TPLGESDIEVVPTKHVEVTEPGAPRTRHDDHPFFQTFEHHYSADPDRIKKQIAADLDL
                     LHRFSEQLNAQRAKGQGKLAGKQSVTDFAGQAPFLDGHVWVHPIQPDRVYDLTVLGDL
                     HGCYSCLKAALLQADFFAKLEAWKLDPTKPEPKLIFLGDYIDRGMFSYNGVLRAIMQL
                     YLAAPDHVFPIRGNHEYYIEYRGRIYGGVKPAEAINTLIGLVPGEVFQEYMKMFEDMP
                     NMLFFDDLMFVHAGIPRDTDIKAKLADMSGLNDADIRFQMLWSDPSTADFIPDDLQQQ
                     NARFPFGKKQFEAFMARFGCSMMVRGHEKVDSGFKTMYGDVASLLSLFSAGGAENHDL
                     PPDSSYRSVTPMAATIRLEGGNTQVTPWLIDYKRFNDPKRNRFFASPPEIEHKVG"
     gene            14378..15685
                     /locus_tag="VMZ53_12555"
     CDS             14378..15685
                     /locus_tag="VMZ53_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS29338.1"
                     /translation="MRVFLAVLVVSTASPAFADPADRLEAGGFLGVDYFGDDIGLGSA
                     LAPEQRPQTAPILGGRLTYVALRTNSDIQLDVGGELELSFAPSWTGYGFEEERPSYFA
                     PVFGFRGNLLARIGSGWLQPHAIVGAGGATVISSSPYMKKETDPVYFWGLGATFPVAG
                     RWQLRFDGIQTRMPARDETGMGDAGTTSTYELRMSVAARFGAGYTAKTDEHVDVVVDN
                     QPPRPPPPDPDKDSDNDGIPDRLDACPAEGELVNGVDDQDGCPEQDPDKDNLVGALDK
                     CPDQPEDVDKFQDEDGCPDPDNDNDGVADANDACPTEMETKNGITDDDGCADQVPKSV
                     TDALTVGKQIKFDPGKQRLKTATKAALDKVAAVLLGNRFLRIRIVVHPDADTEAAKAL
                     ASKRAEAMKWYLNEQGVAPGQLDVATGGVAPKNAPVADLLVAP"
     gene            15682..16239
                     /locus_tag="VMZ53_12560"
     CDS             15682..16239
                     /locus_tag="VMZ53_12560"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HUS29339.1"
                     /translation="MIGESIARALAKLLEIPLALDSVKLEPLDAIVVLGAPLGPGGAL
                     TVVLRERVDAAVALYSAGAAPVVVVSGGVTHGAPRAEADVLADALREAGVSEVIVERA
                     SKTTAENARFTAKLLAARNGRRVWLVTQPFHGKRAALLFRRAGLEPHIWHIEDSLQYR
                     DRRRAVKWLAREWLSWGVLVARGRR"
     gene            complement(16236..16940)
                     /locus_tag="VMZ53_12565"
     CDS             complement(16236..16940)
                     /locus_tag="VMZ53_12565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HUS29340.1"
                     /translation="MRFALACVLPALLFATAQAKPRVVLDPGHGGSNLGAQGVKLREK
                     QLTLTLATEVAARLRDKGIDVTLTRTADRTLTLRQRVEVANKLPADVFVSIHANASPA
                     RTQRGYETFVLTVKGLDIDGRALRSDTTTPRPGVDADIALVLDDVERGAAQWESAELA
                     ARMQKALKARRGNEGDRGVRQDAQHVLLGATMPAVLVEVGFIDHPLEGKELADPRIQA
                     QLADAIAEAIEDQLAD"
     gene            17041..18666
                     /locus_tag="VMZ53_12570"
     CDS             17041..18666
                     /locus_tag="VMZ53_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29341.1"
                     /translation="MSSRRAAGGIAPIHILLLVAFLEVAINRVAVPMLRPLKGTPPTW
                     HTVLDYSGLFLFYFTGTLAAFLIFAHVAKLFERRSPLVLVASIALALAGVLAAVPLVM
                     HVPAALSIPLETVFALAVILLLASALGRHRDVGAQLGLISLALPLLLHTASVYAARYV
                     WPEGAFDGPVTTIVRLGVMALSLAALVSPYVFAPRPFARAVTRPGPVLFAMGIAALGA
                     IIARSFYSKVAKAASLAIGVELSTSHADPRLALYLLAIATLAWTLASCAFAESPARRQ
                     IGSGIALVVLGGYGFHWPNHYLLPLFGFALVADAITRVREEELSGLPIAAETPPIADA
                     AWSAYVGTVKAGVERTLANVHTLTTRGEGGLSSSVIVGDRNGLPVRTRIERIDGGVIA
                     IDVVLGREYDEMRGATLTLWAIPPRELGVNPAGPPAAPVFKTGDVGFDERFRARGNRL
                     SFDRLFDDGLRARIVATLDGWFAYWAPEGMRYRVYPGRGAPLDHPIALSDLALGRTAS
                     PDRLVAVIELLVEIAERGIVRDAAAAPSALDAE"
     gene            18670..20121
                     /locus_tag="VMZ53_12575"
     CDS             18670..20121
                     /locus_tag="VMZ53_12575"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUS29342.1"
                     /translation="MARFTIFTVVALAGCAPLTYEAATPAWVNDTSTLQPKTPTPIAD
                     TYREVAATIIKTARSDRGAYAKLSELTDKVGNRLAGSAALDKAIDWATKTLAADRITV
                     KTEKVMVPHWTRGTEAAAVMSPVSHDLRLLALGGSVATPKPGLTAPVVVVHDWAELDA
                     KAEQVKGAIVVYNVPMPAYSEQEGSGYGKTVAYRTQGASRAAKKGAVAALVRSVTAHS
                     LGTPHTGAMHYDDDAPKIPTAAIAVEDAELIDRLATKGPVTIWLRLDTATYPDAESAN
                     VIGELKGSTNPDEVVVIGAHLDSWDVGQGAHDDGAGVVTMMQALQTLAKLGLHPRRTI
                     RVVLFTNEENGLRGGKTYAEVHAKELPSTVFALEADSGGFPPRGFTVSHKDEAAATRM
                     RGRLADITSLLHELGAMTIRQGGGGADISPMGPAGVPQAGLEVDGRTYFDYHHTEADT
                     LDKVKPQDLSDMVAAIAVVAYVVADMPDRVDAP"
     gene            20365..20565
                     /locus_tag="VMZ53_12580"
     CDS             20365..20565
                     /locus_tag="VMZ53_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29343.1"
                     /translation="MMTVPAARPYRMNADVMELDIAQIIASLEAEQTLVVSRRQVAAF
                     SARKRPRFVQRVLAWFGGRVAS"
     gene            complement(20562..21473)
                     /locus_tag="VMZ53_12585"
     CDS             complement(20562..21473)
                     /locus_tag="VMZ53_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29344.1"
                     /translation="MRRVALVLATLGCGSGSHSPDARDASEGTDADASSSRCDVTKPF
                     GAPSLVEGVNTDEDDRWGWLTADERTMYFARALAGGEGFDLFTATRATASGAFGNVTM
                     LATSTALSEKRPVVSGDGLTLYMEYTTAAGDADLRVATRATLTDEFSAHSSVSVDSPS
                     SNELNPWITSDGLTLYFTSDRDGFNDIFVATRPPTSASFSTPVAVAELNSTAGDYMGA
                     LSGDGLELFFGSSRDTNLANDDIFRATRATPSGPFGAPVKVTELSDAATNEYPTWLSA
                     DRCQLLLTSNRPGGPASYNIWLATRPL"
     gene            complement(21495..21884)
                     /locus_tag="VMZ53_12590"
     CDS             complement(21495..21884)
                     /locus_tag="VMZ53_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HUS29345.1"
                     /translation="MASAAHDHHDAHDDHGIAHTATIKTLVATGGTLLVLTLVTVLAT
                     KIDFGANINLAIAMVIAVTKATLVVLFFMHLRYDRLFHSVIFVSAILAASLFVGFTLM
                     DTGQYQHTNIWHPSAPPSAPVGPRPTP"
     gene            complement(21904..>23077)
                     /locus_tag="VMZ53_12595"
     CDS             complement(21904..>23077)
                     /locus_tag="VMZ53_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HUS29346.1"
                     /translation="LFLAQEVLFFSALFVAYVLYRVHHGELYSYAHKWLDVKYGAINT
                     GVLIFSSLTAAWAVRCAQLGQRKGLILCIVTTIVCAFAFLGIKYIEYSHKYHEQILFG
                     RYFDPCVSPGGNELITKKNECPGANDTIVWDAGAGKAIEGCFEGATIDQNREEEGVQA
                     DCKVFEVKGEHERGADGKMAFKEKGRREITERCHVWSLEEEEALKASGKKEEPEKFPC
                     WKTSYQPAVCPDLTDAAARARGTPGRPQVGILVAYGDHEEREDVLIKAECKEAPKVTQ
                     EIDILGTPEHNQQLGKGTLVKKSRELTTHELHEIESMGPPPEHTNMFFTIYFAMTGLH
                     GIHVLAGIVVFIWLLIRALKGHFTPDYFGPVDYAALYWHIVDLIWIFLFPLLYLIH"
BASE COUNT         3826 a         7514 c         7752 g         3985 t
ORIGIN      
        1 ctcgtcgtcg tcgcgaacac gctcgcttcg cggcagcgtg atctcgggcg cgacgaacgc
       61 ctgctcgatc gccacgtcct gctcgagctg cgcgaccacg ctcgccgcct ccgatgcggg
      121 cacgtcgacg cgcatccagc ggtcgcgcgg atcctggcga ggtggtcccc acgcgcccgc
      181 atacgaaccg tagtgcttcg cgaagagcgg ctgctcgccg agaccgagcg catgcacgtg
      241 ggcctcgcga aacttgatgt ggatcgaagc gcgctggccg tcgacagtgc gcggtgaaga
      301 cgtgccgcgg tcatcggcgc atcgcgtgga ggcggccacg ccggtcaccg cgatcgcaaa
      361 caagacgtac cgcaggacac caggtcgatc ggcgagcatg cggcgcatac ctctatctta
      421 gcagaacgcc ttgtacgcct cggcgcgagc ccgcagtcgc gcgtcgaggg tggcgctcgc
      481 atatcccagc gtgtcggcga tgagcagccc ctcctcgcga tacgccgcgc gcttctcggg
      541 aggccagtaa tgcggcggct ccgagagatt cgtgatgcgg tcggcgagct tcacgaccgc
      601 gatctccgtc ggctgctcgc gaatgcggcg aagcgagtcg agcatcttct cgcgcttcgt
      661 agctgtccgc ccgtcgacga ggagggcgtc gttcttcgag agggccgaca cgccggcggc
      721 gaccgcagcg ccgaatttgg cagtgacctc gtcgagcgtc gtcgccgtgt cttcgacggt
      781 gtcgtgcagc agcgcgcagg tgacggcgag atcggggtcg gcgagcgtgg tcgtcggcaa
      841 tgcggcgatg acttcggcgg cgaccgacac gatgtgcacg agatacggca gctcgctgcc
      901 cggctctttc tgcgcgaggt ggcgttcggc ggcgaagcgc atggccgcga cgtagcggtc
      961 gggcgagtac atgcgtgaag gctagccgat cagctcgcgc atgtacgctt ggagcatcgc
     1021 gccgagcgtt ctttgcacgg cggtgtcgag ctctggcgtt tgctgccggc cgatcgcgcc
     1081 ggagagcgcg tccttcgtga gcgcataccc gagggcggcc gcgacagcgg tcacgagcga
     1141 gagctcgatt ttctcgacga gctcgcgacg cttcgtctcc ggcaccggcg ggcatcccgg
     1201 catcgcgagg aaggcactcg cgacctgatg cgcgacggcc tgcagtccct gttgttgcag
     1261 cgcgaatacc tgcatcgatt cgccacgccc gctcgccatc acccacttca tcaggcgcac
     1321 gtgaaccgga tcgccgagcg tgctgaacag ctgcgcgagc atctcgtcgg gccgctcgag
     1381 agcacccgcg ctgccgagcc gagtcgccac cgtctcgcgc aacctcagga ggcggcgctg
     1441 cagcacggcc tcgaccaagc ccgcgtacgt gccgaagtaa tgcgtgatga gcgcgtggct
     1501 cgtaccggcc tccttcgcga cgtccttgag gccgacatcg tccggccgct cgacgttgaa
     1561 cagacgctcg gcggcgtcca ggagctctag gcgcgcgacc tccggagtcc gccgccggcg
     1621 agcttcgcgt cgctcctctc gctcctcgcg ccgatcggcg cggcgctgcc ggatgggcct
     1681 caactgtttg cgatcacgag ccattgcgga gcacagcgtc gcacacttat tggcaccttg
     1741 acaataacga ctgcgtcgct attatcactg tgacaataag aaatggagcc gaccatgaac
     1801 agctcatcca ccacgcctcc ccgctccacc gaccgcagtc acatcgtcga tgtcgcggac
     1861 ctcccgccgc acattcgctg ggcccgcgcg ctgcgcgccc tcggccggat tttgcgcaat
     1921 cccgaagaga ccgaccaggt gctcgtgttc tcgaacctcg cgaacgcagg ccgccgcgat
     1981 cccgacgagc tgaaccggtt gttcaccgac ccgcaggcgg cgaggctgtt ccgcgagcag
     2041 cgcgcgctcg actcgaagac gatcgatgtc gatgtgctcg ccgcgctgcc cgagggcacg
     2101 ctcggtcgtg cgtacgcgac gttcatgaag gcgcacgggc tgacgcccaa cgtcttcgac
     2161 ggctcgcctg acgacgtccg cgacgagcgc gcggcctact tgatccagcg catgcggcag
     2221 acgcacgacc tgtggcacgt cgttaccaac tgcgagacgg atccggcggg cgagatcgcg
     2281 ctgcaagcat tcacgtacgc gcaagtgcgt gcgccgagct cggcgatcct ggcgctgctc
     2341 ggaacgttga agagcctgcg ctacacgcgc gacgtcgcgc ccgatgcgct cgagctctat
     2401 cgcgcaggcg ccgccgcaaa gccgctcgtg ttcttcccgt gggaggacca ctgggcgacg
     2461 ccgctcgtcg acgtccgccg cctgctcggc cttcccgcga atccgcgcaa ggtcggcggc
     2521 tatttgaccg aagccatgac gctcgccgct tgatggcgag caacgaacag gaactgacat
     2581 gaacatcgaa cgaaaactga tcaagcaact gaagcgcgcc ctcgtgcgcg aaatccgccg
     2641 agcatcgcgt gtcgagcgcc gcgcgctacg tcgcctccgg aacacaatcc gccgctgaac
     2701 aatctcccgc gctcgcaaga aatcggagcg cgacgacgca cggcgtgccg tgctcggggc
     2761 acagctgcgc gtcgatggtc tgcgtgcaac acccccatca tgggctcccg aaacggcgac
     2821 ggcgtgagag cgaagcgacg tcgactgcgt gtgcccgccg cgttggcggt tgcgctcgtt
     2881 ggctcgtcga tgtcgattgc ggcatcggtg gtgggctgta gcccgtccga ggagccgccg
     2941 gtccccgacg cacaaccagg cggcacgatc ggcggtacgc gtcaggacgc tagcgtcagc
     3001 ggagacggtg cgggtagcga agacgcgggc ctgccgcccg atgcgccgag cgtgaccgac
     3061 gcagccctcg acgcgccgct cgagccaacg cccgacgctc cccacacgtg atgacgctgc
     3121 cgcgacccgg tcgggtcgat ttgacgtcgg cgcacggtgt gcctgtggtc cgcacagatg
     3181 ctcgaaaaac gagcgcgtga caaacaccga tcatgaagcg accacgcttg cgaattcccg
     3241 cggtgctggc ggtcgcggtc gtgggcacct cggcgaccgt cacgttgacg ttctcgggct
     3301 gcggagcgga gccgggaccg gagccgatcg atgcaaatcg catcaactcg gtgaaggtcg
     3361 atgcgggtgt cgatgccgtg gacgcccccg acgcgcgtcc gcctatttga tagcgccgag
     3421 gcggccggtt cggctgccac gtggtatcgg aggagcgtga gcgagcagcg ctggatccgg
     3481 tcgcaacgcg cggcggtcgt cgatgcgccg agtcggggca gcggtagtga cggtgacggc
     3541 gatgggctca tcgtcgtcgg gcgcgatgca cgcgtgttca agctcgaggg cgatagtgcg
     3601 cagctcgcgc gcgtcgtcgt tgcattcctc ggacggccgc acagcgaacg cgaggtgctc
     3661 gcgcatctcg aggcgctcgg cggggagccg ctcggcgacc gcacgacggt cgtgcaacag
     3721 ctgctcacca tgctggcgga gaccggcgcg atttcgagcg ctccagcgga cacgccgacg
     3781 acggccgcga atgcgaacga gccgccaccg aaggtgcgcc ccgcagggct caacgtcgtc
     3841 gtcggtatta ccggtgcgat cgcggcgagc tctgcgccgg ggcttgtcac cgcactccag
     3901 cgacgtggcc acaccgtcga gatttgcgtc acgccgaccg cacagcgatt tgtcgcgatc
     3961 gatgcactca ccgcgatcgc gcagcgtgaa gtgcacacat cgatgtggcc gcggacggcg
     4021 cacgcacccg tgccgcatgt cgcgctcgcg cagtgggcgg acctcgtcat cgtgtacccg
     4081 gcgagcggca cgacgatcgc gcggatcgcg catggcgaat tctccgagct cgtcgcagcg
     4141 gtcgcgttga cgacgcgtgc gccggtcgtg ctcgtgccat cgatgaacgc ggagatgctc
     4201 gcggccgcgc ccgtgcagcg caatctcgac cagctgcgcg aggacggctt cgcgatcgtc
     4261 catggcgtgc cgtcgcacga ggtcgctgat gcgccgagcg tgcgcgactc gatcgccgga
     4321 gccgcgcctg cgccgggcga agttgttgcc acgatcgaag cgctgcgcgc agcggccgtc
     4381 ctcgagcgcc gcaccgatga cgcacctgga ccgcgcgcgt gggaggcggt gtatcgcaag
     4441 gcgtctgcgt cggagcccgc agcgaatctc ctgccgtggg cgagcagcga gtgcgatgca
     4501 gacatcgtgc aggcacttgc ggagcatgcg ccgcgcggac gcttgctcga cgttggctgt
     4561 ggccttggcc aagtcgcgcg tcacgcagcg cgcagcaact accgagtcgt cgcgacggat
     4621 gtctccgatt cggcgctcgc gctcgcacgt gacgtcacgc ccgacgacga catcatctgg
     4681 ctgcgcgatg atatctgcgc gagcgggctc gccggcacgt tcgatgtcat cgtcgatcgc
     4741 gcaagcctcc acacattctc gccgctgcgc gcgcatgcgt gggccgcgac gctcgctcgg
     4801 atcacgcgcg ctgggagcac cgtgatcgtg aaggcgcaca aggccggcgt gcctcccgca
     4861 acgcgcggct attcagccca gagcatcgcg gctctgttgc cggacttcgc agtcatcgcg
     4921 gagcgagatg cggagctgcc cggactggtc gacgcaaatc cgatcgcgtc gacgctcgtc
     4981 gtcctgcggc ggcgcggtcg ttaagcggac agcgacatcg cggcggcgat cagcgccgac
     5041 gggcgaacga tgcgcttgcc gatgccgacg ggggcggtag cgcccattgc ggcgtcgaag
     5101 cgcgcacgag ccgaattcgt cgacgacgga gcgcgcatca tgcgaccggg gtgcagaacc
     5161 ggaatcgcct cgttggagat gtacgtgccg ccgggacgac ccgcgacggg caaactgaat
     5221 acgatattgg gaccacgccg aatcatgccc gcgccatgag caagctctgg gccgatccgg
     5281 agggcgcgca accgctcaga tcaggccgtg tttcaattgt aacgggcgct ggtgactagc
     5341 ggagtagccg gtaagctcgc ccgccatgag tcgcgtcgcc atcacgggtg ctagtgggtt
     5401 gctaggaggg aatctggccg cggagctggt cgcggccggg caccaggtcg tcgccacgag
     5461 gcgcgcgggc acgaaggttc gtcacctcga cgatctggcg atcgactggc gtgacggcga
     5521 tctcgcaaac gcgggctcgc tcacgcgcgc gttcgagaat tgcgacgccg tctttcactg
     5581 cgccgccgcg gtcggcgtcg agaagatcgt cacgaaggag atgcgcgaca cgaatgtcgg
     5641 cggtaccgag cgcgtgatcg acgcgtgcgt cgccgcgggc gtgaagcgac tcgtgcacac
     5701 gtcgtcggtc gtcgcgatcg gcctcaccac gagcggcgtg ccgagcgatg agaccgcgac
     5761 gtggaacttc gacaaggaag gcctcgtcga cgcgtatgcg atcacgaagc acgaagcgga
     5821 agaagtcgtg cgcaaggccg cggctgccac gctcgacgcc gtgatcgtca acccgacgta
     5881 catgttcggc ccgcgcgacg cgcgtccttc gagcggcacg ctgatcctca agatcgcgaa
     5941 gcggcgcgtg cccggctgga caccgggcta caacaacttc gtcgacgtgc gcgatgtcgc
     6001 gcgcggcatg atcgcagcgt ggcacaaggg acgccgcggt gagcgctaca tcctcggcgg
     6061 tcacgacatg acctatcgcg cgtgcttcga gacgatcgca aaggtcgcgc gcgtcaagcc
     6121 gccgcgcctc ggcgtgccct acccgctcgc gaagctgctc ggtcggttcg gcgacatcct
     6181 cgaagcgcgc ggccgcgaac cgctcgtgaa ctcgacgcag atcgaatacg cgttcaccga
     6241 caagtttcgg ttccactcga cgaaggccgc cagcgagctc ggctacacgt acggcccgct
     6301 cgagcccgcg atccgcgagg cgctcgattg gttccgggcg aatggaatgc tctagcgcgg
     6361 ctggcgcgtc gtcagatatt tctgcccgcg cgtcatgagg ccgcggaagc cttcgatgtc
     6421 gcccgcgcgc acgacggacc gcagccgctc caccgcgtac atcagcgccg cgagcgattc
     6481 ggtcccgtaa tcgttgagcg tctggatctc gaaatacaaa tcgggattct ccgccgcgac
     6541 tttcgctgcc acgccgagct gcgcgtcgaa cgtcgtcgac gatagctgcg cgagcttcgg
     6601 tgcagcctcg ccgctctctg cgagcgccgt gaagaacgcg atgttgagcg cgtgcgagag
     6661 cccgagcacg tacgcgatca agcgatcgtg actctcgaga tccatctcga cgagcgtcgc
     6721 catcgtcggc tcgaacagtg cgcgagctgc agccgtcgct tgcggcgcgc cgagatcgat
     6781 gaagatgacg tggcgcccgc tgagtagctc ggtatcggga ccgaacatcg gatgcacgct
     6841 cgtgacttgc gcaccggcag cacgcaacgc ggtgagcccc ttgcgcagcg ggctcttgag
     6901 cgacccgaca tcgaacacga cgccctttgg cggctgcttc gccatctcct cgaggatctc
     6961 gttcgtcgcc ggcatcggtg cggcgatcac gatcagctcg tgatcgagcg cgagcgtctt
     7021 ccaatcgcgg tggtacgcga tgtttgcgtg gcgcaccggc ggctccgtcg gatcagcgct
     7081 ctcgacggtg aagccctgcg ctgcgaggta gcgcacgaac cagcgcccca tgtggccgga
     7141 gccgccgatc acgagagcgc gccgccccga gccttcgccg ctggccgcga ccgtgtcctt
     7201 ctcctgcacg gtcagcgagc cgcgaatcag cgcgaggatc agctcctcgc cgagctgcgg
     7261 cgtcaggcca tgctggacgg ctgctgcacg agcgcgctcg accacgtcgc gctcctggcg
     7321 gtagtcccgt gtgggaatgc cgccatcgcg cttcacctgg ccgatcttct gcgcgacggc
     7381 ctggcgcttg gagaccagcg ctaggatctc gcgatcgatc tgcccgagct cctggcgcaa
     7441 cgggtcgagc ttggtcctag catcaccttc ggaggacata gccccgaagg tatcagggac
     7501 gctcagcccg cgtagcggat gacgctcgcg cgaccgagct cttgggtgcg ccagcggtgc
     7561 agcgtgcgga acgcgtcgtg agcagcgacg acgatacggt cagcgacagc gggcgagccg
     7621 gcgacctgct gcatggcaag tccgagctcg cgccacatcg cgccgacttg gcccgcgtag
     7681 cacatgagat acgccgaagc ggtcgccatc tcggtcggca aatacgaagc aaggtggccg
     7741 cgaataaccg cgctcgccag cagcggacgc tctgcgatgt acatccagcc gagcgcagca
     7801 gcgggaccgc ggaacgcagg aatgcgcgtg cagtgcggca gcgcgctgac ttcatccatc
     7861 gtcataccga gcgcgaacaa gtcatgcgcg acgagcgccg acttcgcgcg cccctttgca
     7921 tcgatgacgg tatcgaggtc gggtgccgtc gcgagcgccg cctcgagggg tacgaggaag
     7981 ccatacacgc gctgcaggta cgtgcggtag tcggcagagc tgacgttgga gcagaacaga
     8041 tagcggtcga cgtcggaatc cgcgtccgcg tggtggacct gcgtctcttc gttaaggcgc
     8101 gcgagaatcc gcagctcttc catggccgcg gttgtattgc aatttacgga caaccgagga
     8161 gttgatgcaa atacgcacgg ttaacgcgac tcactatgtg cggctagagc gcgccgcgcg
     8221 ccgctgctac gtgctcccgt gcctcagagg aggattcgta cgtaaggtcg cgctctcaga
     8281 cgggcttgtt tgcgacgacg attttggaga cgaggcgggc ctgcgtctca cgaacgtcga
     8341 acccgatctc gtcgaggatc gctgcgaggt cgtctcgcag atagtccgcg tagaacggct
     8401 cgtggaactc ctgcgggaac ccgaggagca cctctgcaag ctcggggctc tccgacgcct
     8461 gcgctgaatc ttcgagcacg cacaggccac ctggccgcac gacgcgaagc atctcgcgaa
     8521 tgacgttgcg acgcgcattg cgaggaagct cgtggaacat gtacgtgctc gtcacgacgt
     8581 cgaacgtgtt gtcagcccac ggcagtgctt cgccgttttc gactgcgagc gtcagctcgg
     8641 cgacgtcctc gagccgcttg cgcgccagct tgatgtaatt cggcgacagg tcgcagccgt
     8701 gcagtcgcat ctgcggatgc gtacgcgcga tctggtgcag cgtgcgcccc gtaccgcagc
     8761 cgatatcgag cagcttcatc ttcgccgcgc cgccttccgt cgcacgcacg agctcggtga
     8821 tcggcgggat gacctggcgg cgcatcacat cggcggtgcc gcggaacagc agctcgactc
     8881 cgagctcgta gacctgcgcg gagaagtcgc tgaagtagcc atccgtctgc cagtgaaagt
     8941 tgcgccggta gtacggaggg aagcgctcct tgtcgacgtc gggaatgtcc ttgaacgcgt
     9001 tcttgcgcat gcgacgcacg accttgggcg cgtcgaggac gagacgcgga aactcgcgca
     9061 ggtagcggcg caccggcacg tcgaacagca gctcgcgcgg gtagtagccc gcctcgacgt
     9121 tcgcgagatc gatgcgatgc agctcgccgc gccgctcgcg cagcgtcttc catgccgcga
     9181 acggaacgtg cggccgcgga ttgcgcgaga tcagccgcgc tgtctcctcc atcatggtcg
     9241 cgttgaccaa gaaggtcagg tgctgcgagt ggtacgcgag ccgatcgatg cgggacagca
     9301 tggtgctcct ttagcggcga gcggtgggac gccttcttcg acggaatacc aagcctgcga
     9361 caacgaggcc actcagcatg gccgtcgatc ccgacgaccc tgcctcgcag cagcctggct
     9421 tctcgggctt ctgaattccg tcgatggcgc cgtcgatcgt gtcgttgaag cacccgcgcg
     9481 cacccgtgca gtcgacttcc gtggacgtaa tgccgagctg aggcacgaga cagcaccaag
     9541 gcgactgcat gccctcgaag cgctccacgc actgatcgtt ttggaccgtg ccgacagcgc
     9601 acgctacagg cgctttgatg tcgggatatt tcagtacgcc cgtccacgtg ctgaggtcgg
     9661 tggacttcat gatgccgtag ccgtcggcat cgatgttcag ctgcaccgat gccatcatct
     9721 gcgtgcaagc ccagacttcg tgcgcgctgt tctcgtagag acagccgatg tgcggcgccg
     9781 tcgtgagcgg agtccacgtc ggtgtcgccg ctgtcgcgca gtcagccgat ttccaagcgc
     9841 cgagctcacg ggtgccggcg acgcaggtgc cgtcgtaacg gacgaggaac gagagcccga
     9901 acttcgaatc cttcgacagg atcggcgtcc agttggtgcc gccattcgtc gagcgataca
     9961 ccgtgtcgcc gctgctgccc gtctcgaacg tgaccttgac gtacagcgtg ttgggatccg
    10021 tcggcgagat accgacgata tcgatcgtcg agttcgacga cgtcgtgccg atgcccgtct
    10081 tggaaatcgc ggtgtagttg gcgccggaat cgacgctctt gtagagcagg aattctttct
    10141 gcggctcgca catgccgccg ttcgtgcaca tcgcgttcgc cgtgcacgcc gcgcccgcgt
    10201 tgcccgagtt cgccgtgcac ttcatgacga gccggtagcc cgtcaggtac acgcgcgtcg
    10261 gatccgacgg cgccacttcg atcgattgcc accaatcgcc ggcgatgccg cccggtggcg
    10321 tcgtcgacgt cgggaacgtc atcccgttgt cggtcgactt gtagatcttg ctgtcaccga
    10381 tcgtggaatc agacgccgcg acgtagagcg tgccgttcga tgcctcggtg atgcgcgaaa
    10441 cgaacgtcgt gccgaacatc gtcgacgcga agtcgcagcg attgcgcatg accgacagac
    10501 cgtcgaacgt cgtcgcgaag atcgcgtcgc tcgacgtgta ctcgtagtcc ggatcccaca
    10561 tgccaccgta gccgatggcc ttctcgcaca tccattccca cgtcgtgccg ccgtcatgcg
    10621 agatgacgag gccaaacgtc atgcccgaga tgatgttgtt ctcggcgccg cgcttgaagt
    10681 ggatcgtgac ggtgtacggg tcgcggccgt tagcctgggc ggctgatgcc agtgcggtca
    10741 gaaacaaaac cgcgagcgcc ttacgcatag gccgagccta tcccgcgcgc gccggttcag
    10801 gcgaggccga tttcgcgcag tcgctgctcg agatatgcgc ccgccgtcac actcggatac
    10861 ttcgcgaccc ggccggtccg cgcgacgcag ctcggaagcg gcgtcagatc acattcggag
    10921 cgcgcatgca cgaagcacgg gatcgagtag cgctcactcg ttgcatcggg cgggttgacc
    10981 acgcgatgcg tcgtgctctt gtagaggccg ttcgtcaggt tctgcaacat gtcgcctgaa
    11041 tcgacgacga tcgtgtcaaa gcccgtgtgc accggcatcc acgagccatc gcggcggagc
    11101 agctcgagac cttcggcggt cgcgcccgag agcagcgtga tcagattgat gtcctcgtgc
    11161 gccgcggagc gcacggcacc gggcggtacc ttcgagccga cgggcgggta atacagcacg
    11221 cgcacgatcg tgtcgctgtc ctgcgcgagc tccgcaaacc gctccgatgc ctcgccgata
    11281 taggcggcac acgcgcgcag caagttcgtg ccgaggctgt cgagccgttg atagagatcc
    11341 gtcatcgtcg gaccgaagtc agggacctcg gcaggccaga cgttgtcgcc gtattgcggg
    11401 tgattcatcc ccgggcggcc gacttgccag aattccttta ggtcggctgc ctcggagtcg
    11461 cgcgcgtgct cgcgtcccat gccggtgaag ccgcgctgcc ccttgagctt cggatcgtgg
    11521 tagcgctgct tcgcttcgtt tggctgatgg aagaacgcct tcgcgacttc atacgaacgc
    11581 gcgagcaacg tgtcgggcac gccgtgattg cggacagcga agaaaccgat gtcggcgagc
    11641 gattcaccga cggtcgtcac gaatcgctgc cgtgcagctc caccctcgtg ccaatcccca
    11701 agatcaacta ccgggatatt ttgcgaggcc atgcccgccg atattagcgg atcttgaacg
    11761 tctgattcgc gaccttgccg tcgcactcgc agatgtaccc gagctgcatg gacgcgggac
    11821 catcggtcaa gttccgagtc atctcgaaga acgtgatcgt ttcctcggga aacatcgtgc
    11881 cgccgttgtc aggctcgacg ccgttgacct tctcccagat cggcgagctt gcctcgatcg
    11941 gcgtgccggt caccgaggtg aagctgaacg ggatgccgcc ttgcgcgtcg gcgttgcgcg
    12001 cgtagccggt aaagacttgc cagtacggtg gcgacgccgg tacgagcgaa cgcacagcgg
    12061 ccatctccga cggatcgtcg aacacgacga ggtgcgtgat cttcgtcagg ctgtcggtct
    12121 cgcagtcgag ctccgcggca tcccagggca gacctttcgg cgacacgtac cggtacttgc
    12181 tcgtttgggc cgtaccaccg acggctacgt agtcgacggt cgggcacgct tcgagcggtg
    12241 catcggcccc actgtcgacg tgaagcggga cgtgcgtcac gctgaacagc ttgtcgcatc
    12301 cggcgagcgc gacgagcgca atgagccact tcacgcagcc gccctgagct ggcgacgtgc
    12361 gcgcaccacg atcggcgcga tgcgcgtggg ttcggcgacg agcgcgtctt tctcgggacg
    12421 cgtcagctgc ttgatcgcgt actcgatgcg gagcgcgagg tgcttcgtgc gacagcgctg
    12481 cgagaacacg agcgtgaccg gcccgcggcg gcgcgtgtac ttcgcgcctt tgcccacgct
    12541 atgcgcggcg atgcgcgcct cgagatcgtt cgtgatgccg caatacagag tggcgtcggc
    12601 gcagcgaacg agatagacga cccacacagc tatccgactt tgtgctcgat ctccggcggc
    12661 gacgcgaaga atcgattgcg cttgggatcg ttgaagcgct tgtagtcgat gagccacggc
    12721 gtgacctgcg tgttgccacc ttcgagccgg atcgtcgcgg ccatcggcgt caccgagcgg
    12781 tagctcgagt cgggcggcag gtcgtgattc tcggcgccac cggcggagaa cagcgacaac
    12841 agcgacgcga cgtcgccgta catcgtcttg aagccagaat cgaccttctc gtggccgcgc
    12901 accatcatcg agcagccaaa gcgcgccatg aacgcttcga actgcttctt gccgaacggg
    12961 aagcgcgcgt tctgctgctg gagatcatcg gggatgaagt ccgccgtcga cggatcgctc
    13021 cacagcatct ggaatcgaat gtccgcatcg ttgagaccgg acatgtccgc gagcttggct
    13081 ttgatgtcgg tatcgcgggg aatgccggcg tgcacgaaca tgagatcgtc gaagaacagc
    13141 atgttcggca tgtcctcgaa catcttcatg tactcctgga acacctcgcc aggcacgaga
    13201 ccgatcagcg tgttgatcgc ttcggccggc ttcacgccgc cgtaaatgcg accgcggtac
    13261 tcgatgtagt actcgtgatt gccgcggatc gggaacacgt gatccggtgc ggcgagatag
    13321 agctgcatga tcgcgcgcag cacgccgttg tagctgaaca tgccgcggtc gatgtagtcg
    13381 ccgaggaaga tgagctttgg ctcgggcttc gtcgggtcga gcttccacgc ctcgagcttc
    13441 gcgaagaagt cggcctggag cagggcggcc ttgaggcagc tgtagcaacc gtgcagatcg
    13501 ccgaggacgg tgaggtcgta gacgcgatcg ggctggatcg gatgcaccca gacgtgaccg
    13561 tcgaggaacg gagcctggcc cgcgaagtcc gtgacggact gcttgccggc gagcttgccc
    13621 tgccccttcg cgcgctgcgc gttgagctgc tcgctgaacc gatgcaggag atcgaggtcg
    13681 gcggcgatct gtttcttgat gcgatcggga tcggccgagt agtgatgctc gaacgtctgg
    13741 aagaacggat ggtcgtcgtg ccgcgtgcgt ggcgcgccgg gctcggtgac ctcgacgtgc
    13801 ttcgtcggca caacctcgat gtcgctttcg ccgagcggcg tagcgccggt gtcgagcagc
    13861 gcttcgatct cttcgcggaa cttcttctcg gcgggatgca ccgagccgtc tgcgtagatc
    13921 agctcgtcga tcgtcgcgag gagctcgtgt tgattgtcgc gatcgaagct ctggaagatc
    13981 tcgtagctgc ggagcttgag cttgctgtag acgaacttct cgacgtcctc gccgtccgcg
    14041 acggcttcat cgaacaggcc cttcacgcca cggtcgatct gctcgaagac ctcgtggaag
    14101 tgggtcgtga acttgttgat gacctcgtct tttgtcttcg catcggcgtc gggtagcgcc
    14161 gtgttcgcgc gggcggtgac gagctggcga atgtagatcc gcacgaacgt cttctcggtg
    14221 aagtcgaagt cgccgtcgat gtacccgaac gcggtcatgt agaagatgat cgcgttcatc
    14281 tgctgttcgg cgacgtccgg gttcgtgctg aacgtgagca tcgaatcgag cttgccctgc
    14341 ccgcgcgcgc ggctccaggc tgcgatatga ttatttcgtg agggtgttcc tagcagtgct
    14401 ggtcgtcagc accgcatctc cggcgtttgc cgatccggca gaccggctgg aggcgggcgg
    14461 gttcttgggc gtcgattact tcggcgacga catcgggctc ggtagcgccc tcgcccccga
    14521 gcagcggcct caaaccgcgc ccatcctcgg cggtcgcctg acctacgtcg cgctgcgcac
    14581 gaattccgat atccagctcg acgttggcgg ggagctcgag ctgtcatttg cgccgagctg
    14641 gaccgggtac ggattcgagg aagagcggcc gtcgtacttc gcaccggtgt tcggcttccg
    14701 cggcaacttg ctagcccgca taggaagtgg ctggctgcag ccccacgcca tcgtcggtgc
    14761 cggcggcgcg acggtgatct cgagctcgcc gtatatgaag aaggagaccg acccggtcta
    14821 cttctggggc ctcggcgcca cgttcccggt cgcgggtcgc tggcagctcc ggttcgacgg
    14881 gatccaaacg cgcatgcccg cacgcgacga aaccggcatg ggcgacgccg gcacgacgag
    14941 cacgtacgag ctccgcatgt cggtcgctgc gcggtttggc gcgggctaca cggcgaagac
    15001 cgacgagcac gtcgatgtcg tcgtcgacaa tcagccgccg cgcccgccgc cgcccgatcc
    15061 ggacaaagac agcgacaacg acggcatccc cgatcggctc gacgcatgcc cggccgaagg
    15121 cgagctcgtc aacggcgtcg atgatcagga cggctgcccc gagcaggacc cggacaagga
    15181 caatctcgtc ggcgcactcg ataagtgccc cgatcagccg gaagacgtcg acaagttcca
    15241 agacgaggac ggctgccccg atcccgataa cgacaacgac ggcgtcgccg atgccaacga
    15301 cgcgtgtccg acggagatgg agacgaagaa cggaatcacc gacgacgatg ggtgcgcgga
    15361 tcaagttccg aagtcggtca cggacgcgct caccgtcggc aagcagatca agttcgatcc
    15421 gggaaagcag cgcctcaaga cggcaacgaa ggcagcgctc gacaaggtcg ctgcggtgtt
    15481 gctcggcaat cgcttcctgc gcattcgcat cgtcgtgcat cccgatgccg acaccgaggc
    15541 tgccaaggcg ctcgcgtcca agcgcgccga agcgatgaag tggtacctca acgagcaagg
    15601 cgtcgcgccc ggccagctcg atgtggcgac gggtggcgtc gcgccgaaga atgcgccggt
    15661 cgctgacctg ctcgtcgctc cgtgatcgga gagtcgatcg ctcgggcgct cgcgaagctg
    15721 ctcgagatcc cgctcgcgct cgactcggtg aagctcgagc cgctcgacgc gatcgtcgtg
    15781 ctcggagcgc cgctcggacc cggcggagcg ctgacagtgg tgctgcgcga gcgagtggat
    15841 gcggcggtgg cgctgtattc cgcgggtgca gcgccggtcg tcgtcgtgag cggcggggtc
    15901 acacacggcg cgccgcgagc ggaggccgat gtcctcgccg acgcgttgcg cgaagcgggc
    15961 gtctccgagg tcatcgtcga acgcgcgtcg aagacgactg ccgagaacgc gcgattcacg
    16021 gcgaagctgc tcgcggcgag aaatgggcgg cgggtctggc tcgtcacgca accgtttcac
    16081 ggcaagcgtg cggctctctt gtttcggcgc gccggtctcg agccgcacat ctggcacatc
    16141 gaagacagcc tgcagtatcg cgaccgtcgc cgcgcggtga agtggctcgc tcgtgagtgg
    16201 ctgtcgtggg gcgtgctggt cgcgcgcggt cgacgctagt cggccagctg atcttcgatg
    16261 gcttccgcga tcgcatcggc gagctgcgct tggatccgcg gatcggcgag ctctttgccc
    16321 tcgagcggat gatcgatgaa gccaacctcg acgagcacgg cgggcatcgt cgcgccgagc
    16381 agcacgtgtt gcgcgtcttg ccgcacgccg cgatcgcctt cgttgccgcg ccgcgctttg
    16441 agcgccttct gcatgcgcgc cgcgagctcg gccgattccc attgggcagc gccgcgttcg
    16501 acatcgtcga gcacgagcgc gatgtctgcg tcgacaccgg ggcgcggcgt cgtcgtgtca
    16561 ctgcgaagcg cgcggccgtc gatgtcgagg cctttgacgg tcaggacgaa cgtctcgtag
    16621 ccgcgctgcg tgcgcgcagg tgatgcattc gcgtgaatgc tgacgaacac atcggccggc
    16681 agcttgttcg cgacttcgac gcgttggcgc agcgtcaacg tccggtcggc ggtgcgcgtc
    16741 agcgtgacgt cgataccttt gtcccgcagg cgagcggcga cttcggtcgc gagggtcagc
    16801 gtcaactgct tctcgcggag cttgacgccc tgtgcgccga gattcgagcc gccgtgtcca
    16861 ggatcgagga cgacccgcgg ttttgcctgt gcggttgcga agagcaaagc cggcaggaca
    16921 catgcaaggg caaaccgcac ctgagaattc tccgcgagcg gcgaccgaag cggtagtttt
    16981 cggcggtcgg cgcggaacgc cagcttgatc gaagggccca acaaagctac gttttttcgg
    17041 ttgtcttcgc gacgcgctgc agggggaatt gcccccatcc atattctcct gctcgtcgcc
    17101 ttcctcgagg tcgcgatcaa tcgcgtcgcc gtgcccatgt tgcggccgct caaaggcacg
    17161 ccgcccacgt ggcacacggt gctcgattac agcggcctgt tcctcttcta cttcacgggc
    17221 acgctcgcgg cgtttctgat cttcgcccac gtcgcgaagc tgttcgaacg gcgcagcccc
    17281 ctcgtgctcg tcgcgtcgat cgcgctcgcg ctcgccggtg tgctcgccgc cgtgccgctc
    17341 gtcatgcacg tgcccgcggc gctgtcgatc ccgctcgaga cggtgttcgc gctcgccgtc
    17401 atcttgctgc ttgcatcagc gctcggccgg catcgcgatg tcggcgcgca gctcggtctt
    17461 atctcgctcg cgctgccgct gctgctgcac acggcgtcgg tttacgccgc gaggtacgtc
    17521 tggcccgaag gcgcgttcga cggacccgtc accacgatcg tgcgcctcgg cgtgatggcg
    17581 ctcagcctcg ctgccctcgt gtcgccgtac gtgttcgcac ctcgtccgtt cgcgcgcgcg
    17641 gtcacgcggc ccggccccgt cttgttcgcg atgggcatcg cggcgctcgg cgcgatcatc
    17701 gcgcgcagct tctattcgaa ggtcgcgaag gcggcgagcc tggcgatcgg tgtcgagctg
    17761 tcgacgagcc acgccgatcc tcggctcgcg ctgtacttgc tcgcgatcgc gacgctcgcg
    17821 tggacgcttg cttcgtgcgc gttcgcggag agcccggcgc gccgtcagat cggctcgggc
    17881 atcgcgctcg tcgtcctcgg cggctacggc tttcattggc cgaatcacta tctgctgccg
    17941 ctgttcggct ttgcgctcgt cgccgatgcg atcacgcgcg tccgcgaaga ggagctgtcc
    18001 ggtctgccga tcgccgccga gacgccgccg atcgccgatg cagcgtggtc cgcgtacgtc
    18061 ggcaccgtga aagccggcgt cgagcgaacg ctcgcgaacg tccacacgct gacgacccga
    18121 ggcgaaggcg gcctgtcgtc gtcggtgatc gtcggcgacc gcaatggcct gccggtgcgc
    18181 acgcgcatcg aacgcatcga cggtggcgtg atcgcgatcg acgtcgtgct cggccgcgaa
    18241 tacgacgaga tgcgtggcgc gacgctgacg ctgtgggcga tcccgccgcg cgagctcggc
    18301 gtcaatcctg cgggcccgcc agctgctccg gtgttcaaga cgggtgacgt cggcttcgac
    18361 gagcggttca gggcgcgcgg caatcggctc tcgttcgacc gcctcttcga cgatggcctg
    18421 cgcgcgcgga tcgtcgcgac gctcgacggc tggttcgcgt actgggcgcc cgagggaatg
    18481 cgctatcgcg tgtatccagg acgcggcgcg ccgctcgatc atccgatcgc gctctcggac
    18541 ctcgcgctcg ggcgcacggc aagcccggac cgcctcgtcg cggtgatcga gctgctcgtc
    18601 gaaatcgccg aacgcggtat cgtccgcgac gcagcggccg cgcctagcgc gctcgacgcc
    18661 gaataagaca tggctcgctt cacgatcttc acggttgtcg cgctcgcggg ttgcgcgccg
    18721 ctcacgtacg aagcggcaac tccggcgtgg gtcaacgaca cgtcgacgct acagccgaag
    18781 acgccgacgc cgatcgccga tacgtatcgc gaggtcgcgg ccacgatcat caagacagcg
    18841 cgctcggatc gcggcgcgta cgcgaagctg tcggagctga ccgacaaagt aggcaaccgc
    18901 ctcgccggct ccgctgccct cgacaaagcg atcgactggg cgacgaagac gctcgctgcc
    18961 gatcggatca cggtgaagac cgagaaggtg atggtcccgc attggacgcg aggcaccgaa
    19021 gcagccgcgg tgatgtcgcc ggtctcgcac gacctgcgtc tcctcgcgct cggcggctcc
    19081 gtcgcgacgc cgaagcccgg gctcaccgcc ccggtcgtcg tcgttcacga ttgggcggag
    19141 ctcgacgcga aggccgagca agtcaaaggc gcgatcgtcg tctacaacgt gccgatgccc
    19201 gcgtactccg agcaagaagg ctcgggctac ggcaagaccg tcgcgtatcg cacgcaaggc
    19261 gcatcgcgcg cagcgaagaa aggcgcggtg gctgcactcg ttcggtccgt caccgcgcac
    19321 tcgctcggca cgccgcacac gggcgccatg cactacgacg acgatgcgcc gaagattccg
    19381 actgcggcga ttgcggtcga agatgcagag ctcatcgatc ggctcgcgac gaaggggccc
    19441 gttacgatct ggctgcgtct cgataccgcg acgtatccgg acgccgagtc cgcgaatgtc
    19501 attggcgagc tgaagggctc gacgaatccc gacgaggtcg tcgtcatcgg cgcacacctc
    19561 gattcgtggg acgtcggcca aggcgcgcac gacgacggcg cgggtgtcgt caccatgatg
    19621 caagcgctgc agacgctcgc gaagctcggc ctgcatccgc gtcgcacgat ccgcgtcgtg
    19681 ctgttcacga acgaagagaa cggcctgcgc ggcggcaaga cgtacgccga ggtccacgcg
    19741 aaggaactgc cgagcacggt gttcgcgctc gaagcagaca gcggagggtt tccgccgcgc
    19801 ggcttcacgg tgtcgcacaa agacgaggcg gcggcgacgc gcatgcgcgg gcggcttgct
    19861 gacatcacgt cgctcttgca cgagctcggc gcgatgacga tccgccaagg cggcggcggc
    19921 gcggacatct cgccgatggg gccggcgggt gtgccgcaag caggcctcga ggtcgatggc
    19981 cgcacgtact tcgactatca ccacaccgaa gcagacacgc tcgacaaggt gaagccgcag
    20041 gatctctccg acatggtggc ggcaatcgcg gtcgtcgcgt acgtcgtcgc ggatatgccc
    20101 gaccgcgtcg acgcgcccta gaggatcgtg cgacgtcgcg ggcttgggat tcggcgtgat
    20161 ctcgtacgtc gggccgaaaa cttaccccgg ttgttttcct cgtcatccaa gcccgcgttt
    20221 ttcaagcggt caccgcggta gccagcgcgg cttcgattgc gacaggctgc gagcacgtcg
    20281 cgccatgcag tggatgccac cgttgccggt cggacgccgc gtcgcgtggt tacgcgtggc
    20341 gtgggcgttg ctgatatggc gcgcatgatg accgtcccgg ccgcccggcc ctaccgcatg
    20401 aacgccgacg tgatggagct cgatatcgcg cagatcatcg cgagtctcga ggccgagcag
    20461 acgctcgtcg tctcgcgacg gcaggttgcg gcgttctcgg cgcgcaagcg tccgaggttc
    20521 gtccagcgcg tgctcgcgtg gttcggcggg cgcgtagcga gctagagcgg gcgcgtcgcg
    20581 agccagatgt tgtagctcgc aggaccaccg ggccggttgc tcgtcagcag cagctggcac
    20641 cgatcggcgg agagccacgt cgggtattcg ttcgtcgccg catcggatag ctcggtgacc
    20701 ttgacgggtg cgccgaacgg accgcttggt gtcgcgcgcg tcgcgcggaa gatgtcgtca
    20761 ttcgcgaggt tcgtgtcgcg cgacgagccg aagaacagct cgaggccatc gccgctgagc
    20821 gcgcccatgt agtcacccgc tgtgctgttg agctccgcga ccgcgacggg cgtggagaag
    20881 ctcgcggacg tcggcgggcg cgttgcgacg aagatgtcgt tgaatccgtc gcgatccgat
    20941 gtgaagtaca gcgtcaggcc atcgctcgtg atccacgggt tgagctcgtt ggacgacggc
    21001 gagtcgaccg agacggatga atgcgcggag aactcgtccg tcaacgtcgc gcgcgtggcc
    21061 actcgaagat cggcgtcgcc cgccgcggtc gtgtactcca tgtagagcgt gaggccatcg
    21121 ccgctcacga ccgggcgctt ctcgctcagc gcggtgctcg tcgcgagcat cgtgacgttg
    21181 ccgaacgcac ccgatgccgt ggcgcgtgtc gcggtgaaca gatcgaatcc ctcgccgcct
    21241 gcgagcgcgc gcgcgaagta catcgtgcgc tcgtcggcgg tcagccagcc ccagcgatca
    21301 tcctcgtcgg tattcacacc ctcgacgagt gacggcgcgc caaacggctt tgtcacatca
    21361 cagcgcgacg acgacgcatc tgcgtcggta ccttcgctgg catcgcgagc atcgggactg
    21421 tgcgatcccg agccgcaacc gagcgtcgca agaacgagag cgacgcggcg catgcgcctc
    21481 ctcgcagagc ctctttaggg cgtcggccgc ggaccgacgg gggccgacgg cggcgcgctc
    21541 gggtgccaga tgttcgtgtg ctgatactgg ccggtgtcca tcagcgtgaa gccgacgaac
    21601 aacgatgcgg cgaggatcgc ggacacgaag atgaccgagt ggaacaggcg gtcatagcga
    21661 aggtgcatga agaagaggac gacgagcgtc gccttcgtca ccgcgatcac catcgcgatg
    21721 gcgaggttga tgttcgcacc gaagtcgatc ttcgtggcga gaacggtgac gagcgtcaga
    21781 acgagcagcg tgccgcccgt ggcgacgagc gtcttgatcg tcgcggtgtg agcgatgccg
    21841 tgatcgtcgt gagcgtcgtg atggtcgtgc gctgcgcttg ccatgatggg ctccgtcgat
    21901 ccgctagtgg atgaggtaga ggagtgggaa caggaagatc cagatcaagt cgacgatgtg
    21961 ccagtacagc gcggcatagt cgaccggtcc gaagtagtcc ggtgtgaagt ggcccttgag
    22021 cgcgcggatg agcagccaga tgaacacgac gatgcccgcg aggacgtgga tgccgtggag
    22081 gcccgtcatc gcgaagtaga tcgtgaagaa catgttcgtg tgctcgggcg gcggtcccat
    22141 cgactcgatc tcgtggagct cgtgcgtcgt gagctcgcgg ctcttcttga cgagcgtgcc
    22201 tttgccgagc tgctggttgt gctccggcgt gccgaggatg tcgatctctt gcgtgacttt
    22261 gggggcctct ttgcactcgg ccttgatcag cacgtcctcg cgctcttcgt gatcgccata
    22321 cgcgacgagg atgccgacct gcggacggcc gggcgtgcct ctcgcgcgcg cggcagcgtc
    22381 ggtgagatcg gggcagacgg ccggctggta cgacgtcttc cagcagggga acttctccgg
    22441 ctcttctttc ttgccggatg ctttgagcgc ctcttcttcc tcgaggctcc agacgtggca
    22501 gcgctcggtg atctcgcggc gccccttctc cttgaacgcc atcttgccgt cggcgccgcg
    22561 ctcgtgctcg cccttgacct cgaagacctt gcagtccgct tggacgcctt cctcttcgcg
    22621 gttctgatcg atcgtcgcac cttcgaagca gccttcgatc gccttacccg cgcccgcatc
    22681 ccacacgatg gtgtcgttgg cgccggggca ctcgttcttc ttcgtgatca gctcgttacc
    22741 gcccgggctg acgcacggat cgaagtaacg gccgaacaga atttgctcgt gatacttgtg
    22801 cgagtactcg atgtacttga tgccgaggaa cgcgaacgca cacacgatcg tcgtgacgat
    22861 gcagagaatg aggcccttgc gctgaccgag ctgcgcgcag cgaacggccc atgccgcggt
    22921 gagcgacgag aagatgagga cgccggtgtt gatggcgccg tacttcacgt ctagccactt
    22981 gtgcgcgtag ctgtagagct cgccgtggtg cacgcggtag agcacgtacg cgacgaacag
    23041 cgccgagaag aacagcacct cctgcgcgag gaacagc
//
LOCUS       DATLBZ010000235        19847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1737712, whole genome shotgun sequence.
ACCESSION   DATLBZ010000235 DATLBZ010000000
VERSION     DATLBZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19847
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737712"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2352
                     /gene="dnaE"
                     /locus_tag="VMZ53_12600"
     CDS             <1..2352
                     /gene="dnaE"
                     /locus_tag="VMZ53_12600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HUS29347.1"
                     /translation="RFLNPERVSMPDFDVDFCMNRRGEVIKYVQDKYGHDRVGQIATF
                     HQLKARGVIRDIARAMEIPFGEADKLAKLVPEPVQGKSPPVREAIEQTPELKQLYNES
                     PLHRELLDLAASLEGLNRNAGMHAAGIVIAEEPLWEYVPCFRGQNDEIVTQFAMKEVE
                     KAGLVKFDFLGLKTLTVIQTAVKLINQQKPPGEELDITVIPKEDPDVYKMIARGDTTG
                     VFQLESSGFREMLKKLKPDCLEDIVAAGALYRPGPLEGGMVDQYIDVKHGRKAADYMH
                     PWLEPVLKDTYGVIVYQEQVMQIAQVLGGYTLGGADLLRRAMGKKKPEEMAKQKQTFL
                     DGAKNKGVDVKRASEIFELVEKFAGYGFNRSHSAAYGWVTYQTAYLKHHYPHEFMAGL
                     MSCDADNIDNVVKFIAEARSMGLTVERPDINESHQDFTVTPSETAQGGKVIRFGLGAV
                     KGVGGTAVDAILEARSSEGTFTSLYEICRRVDTQKCNRRVLEQLIKSGALDGLPGGHH
                     RAQLLAVLDAALERGASEQRDRKSGQTSLFGLLAPEPIKAPGQAHAQGQDLYPDIEIW
                     GPKQLLAFEKEALGFYVSGHPLDRYRGDLTRYASATTSDFSMGARSVGEHSIGGIVSQ
                     YREMITKKGDKMARFMLEDAGGTLEVIAFPKTFEKTRSVLVSDEPILCSGQVKNEGNS
                     EAPEWKMLLESAAPLSQLREQKTSRVDIHLNADILTPDQVDELKTILANAQRGACTPF
                     VRLKISSRSETVIALPDAWAVSPTEDLLTRLERLFGDRVATLA"
     gene            2349..3149
                     /locus_tag="VMZ53_12605"
     CDS             2349..3149
                     /locus_tag="VMZ53_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29348.1"
                     /translation="MTAAATFCCLECRLLQAPIVDCRECGSAQVAALAFEGEVLRQRF
                     KRQRGKVANAAVQGGLGIGALGAYVGAVAGGVLVSPLVPAVALGALIGGLLIVRKNKR
                     ITTVPFYEPVTAKGAVTKRGVARKLAETIETCDGAERVLAEEATLRGKADGVYFRRSR
                     VARFMVEVEGGERVVVDGVVRVTGPSTRTPAKAGTDPRLVALGIEGVQVAGDLELGVL
                     RDGDPVEVTGIPVEEAVPELAFDRDAGQATVMRGGATAVVVVAVDRRV"
     gene            3271..3621
                     /locus_tag="VMZ53_12610"
     CDS             3271..3621
                     /locus_tag="VMZ53_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29349.1"
                     /translation="MSKATIVSASIYVLLGVACATGGQNGGDIDANTSTHQDASNVIS
                     PDAPRIRMDAAVVTPPQDAQVSQVPQDAPAQSGPFCSANSQCTTAGQCCVTLGGPQGF
                     CGPGTVVLGQCVPQ"
     gene            complement(3645..4655)
                     /locus_tag="VMZ53_12615"
     CDS             complement(3645..4655)
                     /locus_tag="VMZ53_12615"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HUS29350.1"
                     /translation="MARQDRLFADVDRGFTSLEEGEIEEAEKALERCSRIDRHNPDVV
                     ALAAAVADAKGEVDTALAKYTELAELRPDDPMPKVCLARIQLHDVGDADTALETIEKA
                     FDFIDEEDDLVEAIVVRTEALLAVDDAEGARESLAELSTSVIEDPQLALDLGELALAA
                     EDTSGALKWVDLAKKDDATKADAYHLLGRIHEAREERAEMIAAWQEVLKLDAKALPGP
                     VTMSEDELERIATETLHELPDDVRKKLEAVPILIDDLPSEDLINDGLDPRLLGVFNGP
                     AMPDVGSSPAVTNIMLFKKNLERIAHDLDELAEEVRITVLHETAHYFGLDEDDLEKLG
                     LD"
     gene            4692..6557
                     /locus_tag="VMZ53_12620"
     CDS             4692..6557
                     /locus_tag="VMZ53_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HUS29351.1"
                     /translation="MRALLIALSLVVFAAGCHRHTRKTLTPDVPSSGDQEARTRFLEA
                     RSKFLRDGGQAQDFKAIAEDFPSDPIVPWAELYAGIAAVKQRQFPEAEKQLGKVIESN
                     VDPALTARANLFLGIAKNYQGDTEAARRLLANAGNAVENDDERTEFLAAVAYSTAAGD
                     KPLQALGIFDQLYSRVTPTEKALIVVRCQELVASADRNLLERLFDSIADRRGPAIAAV
                     GSRLVMIYAHDGDASREQQMRDNMVPVRAAVGLPRTITESEVGAASGGGGDASIIGAV
                     VPLGSKEENRIAEAAVAGLGLAAGAPDGKGVAAIETRAAIDKTQSAEAVDALARQNAL
                     AIIGPVKGSSVDMAAGRAESLGIPLISLATNADLRTTGKFVFHIRHSPDARARVLAQR
                     ALAAGVKTFAVFAPESDYGKGTSKAFADEITKGGGRIVTTVTYAADTKSFADKTGKLK
                     TGWDAVFIPDDATKLALIAGALAAAGNVSYALPFPKKKLAAGRPVLVLSLAEGLDPSF
                     LASAGRQTVGAMLAPGFYPDAADPIEKPFIDRFVAAYGRQPGPTEAYAFDAAQLAAAA
                     GTGNRAALANALAKAQLQGVTGQIKFDADHRRADPGIVYSVVEETGGVFAIRAVK"
     gene            6585..7274
                     /gene="pyrF"
                     /locus_tag="VMZ53_12625"
     CDS             6585..7274
                     /gene="pyrF"
                     /locus_tag="VMZ53_12625"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HUS29352.1"
                     /translation="MAFSVGNRLIAALDTPTRADADVFVDKLANTPSWVKIGLELFCS
                     EGPSIVRDYTARGLSVMLDLKLHDIPETVARATARVASLGAGLLTVHAGGGRAMLEAA
                     VKNAGEMRVLAVTVLTSLDDADLVDVGAQGPVSELVQRRAQLAVKAGCHGIVASPHEV
                     TSVRANVPEQFLIVTPGVRPAGTAAGDQKRVTTPAQARTAGADMIVVGRPLRDAPDPA
                     AAARAIIAELA"
     gene            7271..8362
                     /gene="rlmB"
                     /locus_tag="VMZ53_12630"
     CDS             7271..8362
                     /gene="rlmB"
                     /locus_tag="VMZ53_12630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HUS29353.1"
                     /translation="MSRPPRGGGRSGGGQRPQGGAGGYRGDGGRGAPTGGAGYRGGEG
                     RGAPTGGGRGDAARGGGEWQREGGRSEGGRGEGGRGDGGRGDGGRGEGGARGDGGGRG
                     GGSSGPGERAVYGVGPVREVVSRRPQSVKALWVDPGRAGKTSGDPVAQIVTAARAAGV
                     RVEDRERQQLEQAAGEGARHQGVVAWLGPFEYAQIEDMVAAAAEQGEPALLVALDGVE
                     DPRNLGAILRSAYLLGAHGVIIPEHRAAQVTAVVAKSSAGASELVPIAQVGNLVRALE
                     EMRDLGLWRVAVHAEMDAKPISEIDGTMPLVLVLGAEGSGVRQLVAKNCDFHALIPMQ
                     RSAVGSFNVSVAGALALYEMRRQRLAKLG"
     gene            complement(8714..10684)
                     /locus_tag="VMZ53_12635"
     CDS             complement(8714..10684)
                     /locus_tag="VMZ53_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HUS29354.1"
                     /translation="MHPFAKPAAVVRASNDEEERLADDSDTIVVDTHDDHASVLDEHH
                     DDHDHLDLSGDDDSIDAHMLELGKRRFGINSFRPGQALAIRNVLAGIDTLAIMPTGAG
                     KSLCYQLPALELDGVTLVVSPLIALMKDQHDKLAELDIDVLRLDSTLSPRDEAAALAR
                     LAEPKPCIAYVTPERLGEPRFRDHLASVKVALFVVDEAHCISQWGHDFRPAYLGLGEA
                     VRALNKPPVLALTATAPPKVKDDILAQLQIPEASVIDIGLDRPNLRYHVIKASSERKK
                     QAMLMRLIDKTPGCGIIYAATVKTVDALADFLWSQGVECGRYHGRMRAKDRERVQTAF
                     MEHSEPRLMVATNAFGLGVDKQDLRFVVHYNFPGSLESYYQEAGRAGRDGAPADCVLL
                     YQPEDKRIQSFFLGGRYPTPEQTRAVAEALVHVTGGKVATETTDDHDPVFVSVKDIAE
                     RAETPAKKARVVLSFLKEVGFAAEAPGARFAPLGLEPPTIEELARAASRYEQKRAQDR
                     ARLASMLRYSQSHLCRTRLLVTYFGYLDAPACGTCDNCQRAARSAPPPSREIEQTEIA
                     ARRNSGEPTTRKQMLEDAMRRRHSAGRGRALKIERVKRPVFGPLEKGDIVRHATWGEG
                     EVVRVVGDSIYTFFPVHGEKLLKANFLEKISA"
     gene            complement(11030..12115)
                     /gene="asd"
                     /locus_tag="VMZ53_12640"
     CDS             complement(11030..12115)
                     /gene="asd"
                     /locus_tag="VMZ53_12640"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HUS29355.1"
                     /translation="MANKLKVGVLGATGMVGQRFVSLLADHPWFEVSAVAASANSAGK
                     TYADAVANRWTLAMDVPAKVAALTVGDASQVSRIAEQCDFVVCAVDMDKAATAKLEED
                     YARAETPVVSNNSAHRWTPDVPMMIPEINADHVAVIEAQRKRLGTKRGFISVKPNCSI
                     QSYVPAIHPLKSFKPTKIAVCTYQAISGAGKTFESWPEMIDNVIPFIKGEEEKSEKEP
                     LKVWGSVQNGQIVAATSPVITAQCIRVPVSDGHMAAVFVAFEKKPSREQILSAWKEFQ
                     GKPQQQKLPSAPTPFLQYFDDDSRPQTKLDRDNGNGQAISIGRLRDDAMFDWRFVALS
                     HNTVRGAAGGAVLTAEFLKSEGFLVAK"
     gene            12159..12644
                     /locus_tag="VMZ53_12645"
     CDS             12159..12644
                     /locus_tag="VMZ53_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HUS29356.1"
                     /translation="MPLLDGLKVVHSSIHGYGVITTRPFKAGELVCYGDGVLYKEDED
                     FDDTYALVLPGEDSGLGEQFFWDLADQTRWFNHSCDPNSEVRSKWDHEANTCRAWWVA
                     LRDIPVGEEITYDYAFVAEVAEPCACGAKTCRGLIVDPDPAELAKLPEHLRQLLRPQA
                     A"
     gene            12650..13738
                     /locus_tag="VMZ53_12650"
     CDS             12650..13738
                     /locus_tag="VMZ53_12650"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HUS29357.1"
                     /translation="MSTRIGILTGGGDCPGLNGVIRAVTLHARNTFGWEVVGIRNGFE
                     GLYEENYVDLHVSTVQHLLGRGGTMLGSSNRANPFAYPVKLPDGRVEIHDVSKRCLTN
                     LEKLDLHGLIVVGGDGTMSFAQKFIDCGMPRIVGVPKTIDNDLEATDYTVGFQTAVEI
                     VVEALERLHTTAESHERIMIVEVMGRYAGWIALTAGLAGGAHVVLIPEIDYDPERVIA
                     AFNRRHGRGVSHSIVVISEGAKPRGGSLSVVTAGDPTKQERLGGAAQRLTDYLCKETE
                     YEVRSTVLGHVQRGGAPCAFDRELATRFGVRAVELIAEGKFGHVAALEAGKLVAKPIG
                     TAVKKLKLVDPNGELVRTAKGIGIELGG"
     gene            13753..15660
                     /locus_tag="VMZ53_12655"
     CDS             13753..15660
                     /locus_tag="VMZ53_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29358.1"
                     /translation="MRRVAWLLILLAACSSKPRRGAALDQPGSCLALPPGLRFHLEPD
                     PRGDGLYWMEAVEYFDYLSGLRSFENLIRYDLRTRTTTVITDHVIKPLLFSDGDVYVR
                     RSFGDKKEFVHIDKGKRVHTLTPDYLDVDDVLQLDETTFALLADGDGARAVYILATDR
                     PRPRYLAEANALVGHDGTHVLLQKGAEYLSVDVATGAATPFKNVPGATVLGPRAFLVE
                     NDQIHVHHLIKGDDYVAVDKKNQWKLVFQRDSMLVRTAPKDDRSEAFLIEDGKATPLP
                     SVSGGTSIYGTAKLGDRLWALIGHNTANSILDPADTVTESEVCALPAGGPVIFKSRIV
                     PGRFLAKSDKLMGALIAAAPGAGVQIAQDYNYPVAVSVTMQTEYARRDFLKMRARVSE
                     IHEAATRLLEDPDVRTEVTWADGWIAIKAWRRDRLREAISVGMGDALLSNPLDVDVIV
                     TDLVDAHVDNGFECSGSLTNTHAEKLENLELRCGTDRMHTIHVAAIGPHETVKFSKVW
                     PQGDEDTWISPLFEITRNGELMQMIDDYHEKQTERLLDLCERQYAASKFTLLTHTIDR
                     DIALSFWAPAEFVDYTEAEQLTIVRDLYRRFREVAKIYDLPTTTPMTITISVHMHTET
                     YDYDGTELTKR"
     gene            15681..16703
                     /locus_tag="VMZ53_12660"
     CDS             15681..16703
                     /locus_tag="VMZ53_12660"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HUS29359.1"
                     /translation="MSDLVQRDEVRPEVNAWDAPLPDPNIQDARIAHTFAPLAGVPVV
                     GRVEAAYRQRFRLKSWQYMTAVTDELFIAFVVGTAGFASNGFVYAAELATGRVHKKFA
                     ITPLMVGTELAPSSTQGMHRFATRGLGVTIENHGRAFDARVDAKTDGGGKLVADLAFS
                     SRKNDEHLAICVPLPGKRWNYTHKFAAFDVTGSVTIDDRRIDFAPGRSFGTMDFTKMY
                     ALRHAVWRWIALCGRTKEGRIVGLNLVDPTPDAPISENALWVDGKRQALTNVRIGADD
                     AQADGVSIQLREVAQVEQKLDVPLVRHRLRHVIGSFSGSVTTQTGEKLDVKDVVGIYE
                     DYDTWW"
     gene            16867..18687
                     /locus_tag="VMZ53_12665"
     CDS             16867..18687
                     /locus_tag="VMZ53_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29360.1"
                     /translation="MFGVQRIGRYEVLSHLASGGMGQVYLARATGLGGFERQVVVKTL
                     DLSITDDDDSFVTMFLDEARLVGALHHQYIAPVYEVGCDEEGRYFLVMDYVHGETAEA
                     VFRDANERKHAIPLSFALTVVSCVASALDYAHSLCAADGTPLEIVHRDISLSNVMIGY
                     DGGVKLIDFGIAKASNRTTKTQVGTLKGKIGYLAPEQILRKHVDHRADIFSLGIVLYE
                     LTTQARAFKAGSDLITLERITKGDIVAPSKIVPDYPRELEAIVMRALEVDPDERFQDA
                     GEMGRALESLAEHKGLRLGHGPVIDVMRALFAEKRPRRRLARSSDELRKQNQAIEADA
                     DEDTDVRSSHPRSHKPTLPMKPSPTSMPAVVPPSGADDDRATVPIMARGTRDGMVPMD
                     HPSRLPTPLPIEEQSTGVVDPNLEVRFAHAKIPLEDRPTPIADASLIDPPTHRDAKPL
                     VIDDTQPRERLTPPERPSGNGIAVPRTLTGQPPPPTRRATSQPPPIPAGIPPFPRSVT
                     GPPLPPHLEDAPTEIAAMRTEREDPTDVSGVEPLPRTTTQLTPTAQLALRQKWGPAYV
                     ATAVVNRYRKKSRDTATQTWFILLGLFGVAVITAMLVALL"
     gene            complement(18684..19502)
                     /locus_tag="VMZ53_12670"
     CDS             complement(18684..19502)
                     /locus_tag="VMZ53_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29361.1"
                     /translation="MARVYLFVTYRNDLEALQARKVALDAAVDSATRERDATAQILEE
                     AKARTRLPVLPNLRIASPCTERWDAMSGDERVRHCGKCDKDVFDLSALTREQAESLIR
                     ERAGDLCARYYQRKDGTILLADCEVGARGVRRRNYLAAGIAVTLAGGTAGAIALSAND
                     PPEQVGSLSTDELEHTMGKIDFGSPVEPQHWTKTGIDECDAYGDSLYSYFWCDSVTAD
                     TGATMLDGFRQSEEAWKLVPPDQRSKIAESCTSAREELESRAHAAGCELHRFFR"
     gene            complement(19560..>19847)
                     /locus_tag="VMZ53_12675"
     CDS             complement(19560..>19847)
                     /locus_tag="VMZ53_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29362.1"
                     /translation="DEVCRAICDRFDHLIRLYYDPNDRQRGYIETKDRFGVQRKFPIM
                     SVSIAGISLSRSKTYAGLAELAAVGKGTAKAIPGSSYVRDGQTMTADAGTS"
BASE COUNT         3415 a         6500 c         6599 g         3333 t
ORIGIN      
        1 cgcttcttga atcccgagcg cgtgtcgatg cccgacttcg acgtcgactt ctgcatgaat
       61 cgccgcggcg aggtcatcaa gtacgtccaa gacaagtacg gccacgaccg cgtcggccag
      121 atcgcgacgt tccaccagct caaggctcgc ggcgtcatcc gcgacatcgc gcgcgcgatg
      181 gagattccgt tcggcgaggc cgacaagctc gcgaagctcg tgcccgaacc cgtgcagggc
      241 aagtcgccgc cggtgcgcga agcgatcgag cagacgcccg agctcaagca gctctacaac
      301 gagagccccc tgcatcgcga gctactcgat ctcgccgcat cgctcgaggg cttgaaccgc
      361 aacgcaggca tgcacgccgc cggcatcgtc atcgccgagg agccgctgtg ggagtacgtg
      421 ccgtgcttcc gcggtcagaa cgacgaaatc gtcacgcagt tcgcgatgaa ggaggtcgag
      481 aaagccggcc tcgtgaagtt cgacttcctc gggctcaaga cgctcaccgt cattcaaacc
      541 gcggtgaagc tgatcaacca gcagaagccc cctggcgaag agctcgacat cacggtcatc
      601 ccgaaggaag accccgacgt ctacaagatg atcgcgcgcg gtgacacgac cggcgtgttc
      661 cagctcgagt cgagcggctt ccgcgagatg ctgaagaagc tcaagcccga ctgtctcgag
      721 gacatcgtcg cggccggtgc gctctatcgt ccgggcccgc tcgaaggcgg catggtcgat
      781 cagtacatcg acgtcaagca cggccgaaag gctgccgact acatgcaccc gtggctcgag
      841 ccggtgctca aggacacgta cggcgtcatc gtctaccaag agcaggtgat gcaaatcgcg
      901 caggtgctgg gcggctacac gctcggcggc gcagacctgc ttcgtcgcgc catgggcaag
      961 aagaagcccg aggagatggc gaagcagaag cagacgttcc tcgacggcgc gaagaataaa
     1021 ggcgtcgacg taaagcgcgc gtctgagatc ttcgagctcg tcgagaagtt cgccggctac
     1081 ggcttcaacc ggtcgcactc cgccgcgtac ggctgggtca cgtatcaaac cgcgtatctc
     1141 aagcaccact acccgcacga gttcatggcg ggcctcatgt cgtgcgacgc ggacaacatc
     1201 gacaacgtcg tgaagttcat cgccgaggct cggtcgatgg gcctcaccgt cgagcgtccc
     1261 gacatcaacg agtcgcatca ggacttcacg gtgacgccgt cggagaccgc gcaaggcggc
     1321 aaggtgatcc ggttcggtct cggcgcggtg aagggcgtcg gcgggacggc ggtcgatgcg
     1381 atcctcgagg cgcgctcgtc ggagggtacg ttcacgtcgc tgtacgaaat ctgccgccgc
     1441 gtcgatacgc agaagtgcaa tcgccgcgtg ctcgagcagc tgatcaagag cggcgcgctc
     1501 gacggactgc cgggtggtca ccaccgcgcg cagctgcttg cagtgctcga tgcagcgctc
     1561 gagcgtggcg cgtccgagca gcgcgatcgc aagagcggcc agacatcact gttcggcttg
     1621 ctcgcacccg agccgatcaa agcgccgggc caggcgcacg cgcagggcca ggacctctat
     1681 cccgacatcg agatctgggg cccgaagcag ctgctcgcgt tcgagaagga agccctcggc
     1741 ttctacgtgt cggggcatcc gctcgatcgc tatcgaggcg acctcacgcg ctacgcatct
     1801 gcgacgacga gtgacttctc gatgggcgca cgcagcgtcg gcgagcactc gatcggcggc
     1861 atcgtcagcc agtaccgcga gatgatcacg aagaagggcg acaagatggc ccgcttcatg
     1921 ctcgaagacg cgggcggcac gctcgaagtg atcgcgtttc cgaagacgtt cgagaagacg
     1981 cgctcggtgc tcgtttccga cgagccgatt ctctgctcgg gccaggtcaa gaacgagggc
     2041 aactccgaag cacccgagtg gaagatgctg ctcgaatccg cggcgccgct ctcgcagctg
     2101 cgcgagcaga agacgtcgcg cgtcgacatc catctcaacg ccgacatcct cacgcccgat
     2161 caagtcgacg agctgaagac gatcctcgcg aacgcccagc gcggtgcgtg cacgccgttc
     2221 gtgcggctca agatctcgtc gcgctccgag acggtgatcg cgctgcccga tgcgtgggcg
     2281 gtgtcgccga ccgaggatct gctcacgcgg ctcgagcgac tgttcggcga tcgcgtcgcg
     2341 acgctcgcgt gacggctgca gcgacgttct gctgcctcga gtgccgcttg cttcaggcgc
     2401 cgatcgtcga ctgtcgcgag tgtggctcgg cgcaggttgc tgcgctcgcg ttcgaaggcg
     2461 aggtgctgcg ccagcggttc aagcgacagc gcggcaaggt cgcgaatgca gccgtgcaag
     2521 gcggcctcgg tatcggcgcg ctcggtgcct acgtaggcgc ggttgcgggt ggcgtgctcg
     2581 tgtcgccgct ggttccggcc gtcgcgcttg gcgcattgat cggcggcctg ctgatcgtcc
     2641 gcaagaacaa gcgcatcacg acggtgccgt tctacgagcc ggtgacggcg aagggcgccg
     2701 tgacgaagcg gggcgttgcg cgcaagctcg ccgagacgat cgagacgtgc gatggcgccg
     2761 agcgcgtgct cgccgaggaa gccacgctgc gcggcaaagc cgacggcgtc tacttccgcc
     2821 gcagccgcgt ggcgcgcttc atggtcgagg tagaaggcgg cgaacgcgtc gtcgtcgacg
     2881 gtgtcgtccg cgtgaccgga ccgagcacgc gcaccccggc aaaggctgga acagacccgc
     2941 gcctcgtcgc gctgggaatc gagggcgtcc aggtcgccgg cgacctcgag cttggcgtgc
     3001 ttcgcgatgg cgatcccgtc gaggtcactg ggatccccgt cgaggaagcg gtcccggagc
     3061 tcgccttcga tcgcgacgct gggcaagcga ccgtgatgcg cggcggcgcg acagccgtcg
     3121 tcgtcgttgc cgtcgacagg cgcgtttaag cgccgcgttg gcgcgtcttg gtccgaccgg
     3181 cgtgcacgag ttgcgcataa cgcaggtggc cacgaagaaa gtcgttgagg tctacaaagc
     3241 cggagatagg atgcgcggcg agggtagcga atgtccaaag cgacaatcgt cagcgcatcg
     3301 atctacgtac tgctcggcgt tgcgtgcgcg accggcggac aaaacggcgg cgacatcgac
     3361 gcgaacacat cgacgcatca agacgcgtcg aacgtgatct cgccggacgc tccgcgcatc
     3421 cgcatggacg ccgcggtcgt cacaccgccg caggatgcac aggtctcgca ggttccgcag
     3481 gacgcacccg cgcaatcggg tccgttctgc tctgcgaaca gccagtgcac gactgcaggc
     3541 caatgctgcg tcacgctcgg tggcccacag ggcttctgtg gtccgggcac cgtcgtgctc
     3601 ggccagtgcg tgccgcagta acgcaacgac gcacgacgag ctaactagtc gaggccgagc
     3661 ttttcgagat cgtcttcgtc gaggccgaag tagtgcgcgg tctcgtgcag cacggtgatg
     3721 cgcacttctt ccgcgagctc gtcgaggtcg tgcgcgatcc gctcgaggtt cttcttgaac
     3781 agcatgatgt tcgtgacggc cggcgagctg ccgacgtcgg gcatcgcggg gccgttgaac
     3841 acgccgagca ggcgagggtc gaggccgtcg ttgatgagat cttcggacgg caggtcgtcg
     3901 atgaggatcg gcaccgcctc gagcttcttg cgaacgtcgt cgggaagctc gtgcagcgtt
     3961 tcggtcgcga tgcgctcgag ctcgtcttcg gacatcgtga cggggccggg cagggctttc
     4021 gcgtcgagct tcagcacctc ctgccacgcc gcgatcatct cggcgcgttc ttcacgcgct
     4081 tcatggatgc gaccgagcag gtgatacgca tcggccttcg tggcgtcgtc cttcttcgcg
     4141 aggtccaccc acttgagggc gccgctcgtg tcttctgctg caagagcgag ctcgcctaga
     4201 tcgagcgcca gctgcggatc ttcgatgacc gacgtcgaga gctcggcgag cgattcgcgc
     4261 gcgccttcgg catcgtcgac ggcgaggagt gcctcggtgc gaacgacgat cgcttcgacg
     4321 aggtcgtcct cttcatcgat gaagtcgaac gccttctcga ttgtctcgag ggctgtatcc
     4381 gcatcgccga cgtcgtgcag ctggatgcgc gcgaggcaga ccttcggcat cggatcatcg
     4441 ggacgcagct ctgcgagctc ggtgtacttc gcgagtgccg tatcgacttc gcccttggcg
     4501 tctgcgaccg ccgcggcgag cgccacgacg tccgggttgt ggcggtcgat cctggaacaa
     4561 cgctcgagag ccttttctgc ttcctcgatt tcaccttctt cgagggaggt gaagccgcga
     4621 tcgacatcgg cgaacaaccg gtcctggcga gccatgggcc ttccatacct cgatttgcta
     4681 ctgtcaccag tgtgagagca ctcctgattg cactgagcct ggtcgtcttc gctgctggct
     4741 gccaccggca cacgcgaaag acgctcacgc ctgacgtgcc gtcgtcgggt gaccaagaag
     4801 cgcgcacgcg ctttctcgaa gcccgcagca agttcctccg cgacggcggc caggcgcagg
     4861 acttcaaggc gatcgccgaa gacttcccga gtgaccccat cgtgccgtgg gccgagctct
     4921 acgcaggcat cgccgcggtg aagcagcgcc agttccccga ggccgagaag cagctcggca
     4981 aggtgatcga gtcgaacgtc gatccggcgc tcacggcgcg cgcgaatctg ttcctgggca
     5041 tcgcgaagaa ctatcagggc gataccgaag cggcgcgcag gctgctcgcg aacgcgggca
     5101 acgccgtcga gaacgacgac gaacgtacgg agtttctcgc cgctgtcgcg tactcgacgg
     5161 ccgcaggcga caagccgctg caggcgctcg gcatcttcga ccagctctac tcgcgcgtca
     5221 cgccgacgga gaaggcgttg atcgtcgtgc gctgccaaga gctcgtcgcg agtgccgatc
     5281 gcaacctgct cgagcgcctg ttcgactcca tcgccgatcg cagaggcccg gccatcgccg
     5341 cggtcggcag ccggctcgtg atgatctacg cgcacgacgg cgatgcatcg cgcgagcagc
     5401 agatgcgcga caacatggtg ccggtgcgcg ccgccgtcgg cctgccgagg acgatcaccg
     5461 agagtgaagt cggcgccgca tcgggcggtg gcggagacgc ctccatcatc ggagcagtcg
     5521 tgccgctcgg cagcaaggaa gaaaaccgca tcgccgaagc agcggtcgcg ggcctcggcc
     5581 tggccgccgg cgcgcccgac ggcaaaggcg tggccgcgat cgagacgcgc gctgcgatcg
     5641 acaagacgca gtccgctgaa gccgtcgatg cgctcgctcg ccagaacgcg ctcgccatca
     5701 tcggccccgt caaaggctcg tcggtcgaca tggcggcagg ccgcgccgag agccttggca
     5761 tcccgctcat ctcactcgcg acgaatgccg acctccgcac gaccggcaag ttcgtctttc
     5821 acatccgcca ctcgcccgac gcgcgcgcgc gtgtgctcgc gcaacgagcg ctcgctgccg
     5881 gcgtcaagac attcgccgtc ttcgcgcccg agtccgacta cggcaaaggc acgagcaagg
     5941 ctttcgccga cgagatcacg aaaggcggtg gccgcatcgt caccaccgtc acgtacgcgg
     6001 ccgacacgaa gtcgttcgcc gacaagacgg gcaagctgaa gaccggctgg gacgcagtct
     6061 tcatccccga cgacgccacc aagctcgcgc tcatcgcggg cgcgctcgcc gccgcaggca
     6121 acgtctccta cgcgctgcca ttcccgaaga agaagctggc ggccggccgc cccgtgctcg
     6181 tcctctcgct cgccgaaggc ctcgacccga gcttcctcgc atccgccggc cgccaaaccg
     6241 tcggcgccat gctcgcgccc ggcttctacc cagacgccgc cgacccgatc gagaagccgt
     6301 tcatcgatcg cttcgtcgcc gcctacggcc gccaaccggg cccgacggaa gcctacgcct
     6361 tcgacgccgc acagctcgcc gctgccgccg gcaccggcaa ccgcgccgcg ctcgcgaacg
     6421 cgctcgcgaa agcgcagctc caaggcgtga ccggccagat caagttcgac gccgaccacc
     6481 gccgcgccga tcccggcatc gtctactccg tcgtcgaaga gacgggcggc gtattcgcaa
     6541 tccgcgccgt gaagtagccg cccactccgt ggtatctccg cggcgtggcg ttctccgtcg
     6601 gtaatcgcct aatcgcagcg ctcgataccc ccacgcgtgc cgacgccgac gtcttcgtcg
     6661 acaagctcgc gaacaccccc agctgggtaa agatcggcct cgagctgttc tgctccgaag
     6721 gcccatcgat cgtgcgcgac tacaccgcgc gcggcctctc ggtgatgctc gacctcaagc
     6781 tgcacgacat ccccgaaacc gtcgcgcgcg caacagcccg cgtcgcgtcg ctcggcgccg
     6841 gcctactcac cgtccacgcc ggcggtggcc gcgcaatgct cgaagccgca gtaaaaaacg
     6901 cgggcgagat gcgcgtcctc gcggtcaccg tcctcacatc gctcgacgac gccgacctcg
     6961 tcgacgtggg cgcgcaaggg ccggtcagcg agctcgtgca acgccgcgcg cagctcgcag
     7021 tcaaagccgg ctgccacggc atcgtcgcat cgccgcacga ggtgacgagc gtgcgcgcaa
     7081 acgtgcccga gcaattcctc atcgtcacac caggcgtgcg ccccgcaggc acggccgcag
     7141 gcgatcaaaa acgcgtgacg acgcccgcgc aagcgcgcac cgccggtgca gacatgatcg
     7201 tcgtcggtcg tccgttgcgc gacgcgccag accccgcagc cgctgcacgc gcgatcatcg
     7261 ccgagctcgc atgagcaggc ccccgcgcgg cggaggacgt agtggcggcg gccagcgccc
     7321 gcaaggcggc gccggtggat atcgcggcga tggtgggcgt ggcgcgccga cgggcggcgc
     7381 cggatatcgc ggcggcgaag gtcgtggcgc gccgacgggc ggcggacgtg gtgatgccgc
     7441 gcgcggcggt ggcgaatggc agcgtgaagg cgggcgcagc gaaggcggac gcggcgaagg
     7501 cggacgcggc gatggcggac gcggcgatgg cggacgcggc gaaggcgggg cgcgtggcga
     7561 tggcggcggg cgtggcggtg gctcgtcggg acctggcgag cgcgcggtct acggcgtcgg
     7621 gcctgtgcgc gaggtggtgt cgagacggcc gcagtcggtg aaagcgctgt gggtcgatcc
     7681 aggacgcgcg gggaagacga gcggcgatcc tgtcgcgcag atcgtgacgg ccgcgagagc
     7741 agcgggcgtg cgcgtcgagg atcgcgagcg ccagcagctc gagcaggccg caggcgaggg
     7801 cgcgcggcat caaggcgtcg tggcgtggct gggcccattc gagtacgcgc agatcgagga
     7861 catggtcgca gcggcggcgg agcagggtga gcctgcgttg ctcgtcgcgc tcgacggcgt
     7921 cgaggatccg cggaatctag gcgcgatcct gcgttcggcg tacttgctcg gcgcgcacgg
     7981 cgtgatcatt cccgagcatc gcgcggcgca agtgacggct gtcgtcgcga agtcgtcggc
     8041 cggtgcgagt gagctcgttc ctatcgcgca ggtcggcaat ctcgtgcgcg cgctcgagga
     8101 aatgcgcgac ctcgggctgt ggcgcgttgc cgtccacgca gagatggatg cgaagccgat
     8161 cagcgagatc gacggcacca tgccgctcgt gctcgtgctc ggcgccgagg gctcgggtgt
     8221 gcggcagctc gtcgcgaaga actgcgactt tcacgcgttg attccgatgc agcgcagcgc
     8281 cgtcggctcg ttcaacgtgt cggtcgccgg tgcgctcgcg ctgtacgaaa tgcgtcgcca
     8341 gcgcctcgcg aagctcggct gagcggctcg caccgggatg cggacgcaga tcgatcggga
     8401 tccgcagctc cgatcggcgg ctcggaatcg cagtcggagt cactcggaga cggcctcgga
     8461 agacggactc ggaggcggac tcggagacgg actcggagac ggactcggag acggactcgg
     8521 agacggactc ggaggcggat cggattcggt gccggacgcg gctcggactc gcagccagac
     8581 gcggcgcgcg tggacaccgc ctcgggccca cgtgggcacg gacgactcga ctccactcaa
     8641 cccggatccg gactcggact cggactcggg ctcggacacg gatccggact cggcgtcgga
     8701 ctcggatccg gcgttaggcg ctgatcttct cgaggaagtt cgctttgagc agcttctcgc
     8761 cgtgcacggg gaagaacgtg tagatgctgt cacccaccac gcgcacgacc tcgccttcgc
     8821 cccacgtggc gtgacggacg atgtcgccct tctcgagcgg gccgaagaca gggcgcttca
     8881 cgcgctcgat cttcagcgca cgaccgcggc ctgcggagtg gcggcggcgc atcgcgtctt
     8941 cgagcatctg cttgcgcgtc gtcggctcgc cactgttgcg acgtgctgcg atctcggttt
     9001 gctcgatctc gcgcgacggc ggcggtgcgc tgcgcgcggc gcgctggcag ttgtcacatg
     9061 tgccgcatgc cggtgcgtcg aggtagccga agtacgtgac gagcagacgc gtcctgcaga
     9121 gatgcgactg cgagtagcgc agcatcgagg cgaggcgtgc gcgatcttgc gcgcgctttt
     9181 gctcgtagcg gctggcggcg cgcgcgagct cttcgatggt ggggggctcg aggccgagcg
     9241 gtgcgaagcg cgcgcccggg gcttcggctg cgaagccgac ttccttcagg aaggagagga
     9301 cgacgcgagc ttttttggcc ggcgtttcgg cgcgctcggc gatgtctttg acggagacga
     9361 agactggatc gtgatcgtcc gtggtttcgg tcgcgacctt gccgcccgtg acgtgcacga
     9421 gggcttcggc gacggcgcgc gtttgctccg gcgtcgggta gcgaccaccg aggaagaagg
     9481 attggatgcg cttgtcttcg ggctggtaga ggaggacgca gtcggcgggc gcaccgtcgc
     9541 ggcctgcgcg gccggcttct tggtagtagc tctcgagcga gcccgggaag ttgtagtgaa
     9601 cgacgaagcg gaggtcttgc ttgtcgacgc cgagaccgaa ggcgttggtg gcaaccatca
     9661 ggcgtggctc ggagtgctcc atgaacgcgg tctgcacgcg ctcgcgatct ttggcgcgca
     9721 tccggccgtg gtagcgaccg cattccacgc cttgcgacca gaggaagtcg gcgagggcgt
     9781 cgaccgtctt caccgtggcg gcgtagatga tgccgcagcc cggcgtcttg tcgatcaggc
     9841 gcatcagcat cgcttgcttc ttgcgctcgc tcgacgcttt gatgacgtgg tagcgcaggt
     9901 tcggtcgatc gagaccgatg tcgatgacag aggcctcggg gatctggagc tgcgcgagga
     9961 tgtcgtcttt gactttgggc ggcgcggtgg ccgttagcgc gaggacgggc ggcttgttga
    10021 gcgcgcgcac cgcttcgccg aggccgaggt aggccgggcg gaaatcgtga ccccactgcg
    10081 agatgcagtg cgcttcgtcg accacgaaga gggcgacctt cacgctcgcg aggtgatcgc
    10141 ggaagcgcgg ctcgccgagg cgctccggcg tgacgtatgc gatgcacggc ttgggctcgg
    10201 ctagacgcgc gagcgccgcc gcttcatcgc gaggcgagag cgtcgagtcg aggcggagga
    10261 catcgatgtc gagctcggcg agcttgtcgt gctggtcctt catcagtgcg atgagaggag
    10321 agacgacgag cgttacgccg tcgagctcga gcgcgggcag ctgatagcag agcgacttgc
    10381 cagcgccggt cggcatgatc gcgagcgtgt cgatacctgc gagcacgttg cgaatcgcga
    10441 gcgcttgacc gggacggaat gaattgatgc cgaaccggcg cttgccgagc tcgagcatgt
    10501 gggcgtcgat cgagtcgtcg tcgccggaga ggtcgaggtg gtcgtggtcg tcgtgatgct
    10561 cgtcgagaac gctggcgtgg tcgtcgtgcg tatcgacaac gatcgtgtcg gagtcgtctg
    10621 ccaggcgctc ttcttcgtcg ttcgacgctc gcacgacagc agccggcttc gcgaacgggt
    10681 gcacgttgtc gcgcgtgcct ctggcgatct tcggcaatcc cgccggtggc gtcgtttcga
    10741 gagacgaaga ggctttccga cgcctctcat cgaccgtact tctcggcatc aaacggtacc
    10801 gtcgcgcatc tcgctctgtg ggcgcaagcg aaagaaagcg agtctgaagt tagcgaaaaa
    10861 atcatttcgt gcgcgccgat cacgctgtgc gctggtgcgc ggtgatctcg cgtgtcctac
    10921 atctgtacgg gcgcgaaacc gctgctcttc agcaccttaa ggaaaaagtg cgaggacgaa
    10981 tacgaaacaa gcatcagcgg atctgctgca acaaactgct gcagcgacgc tatttcgcga
    11041 cgaggaaccc ttcgctcttc aaaaactccg cggtgagaac tgcaccacct gcagcgccgc
    11101 gcacggtgtt gtgcgagagc gcgacgaagc gccaatcgaa catcgcgtcg tcgcgcagcc
    11161 ggccgatcga gattgcttgg ccgttgccgt tgtcgcgatc gagcttcgtc tgtgggcgcg
    11221 agtcgtcgtc gaaatattgg aggaacggtg tcggggcgct cggcagcttc tgctgctgcg
    11281 gcttgccctg gaactctttc cacgcggaga gaatctgctc gcgcgacggc ttcttctcga
    11341 acgcgacgaa cacagctgcc atgtggccat cgctgaccgg cacgcggatg cactgcgccg
    11401 tgatgaccgg ggaggtcgcc gcaacgatct gaccgttctg cacggagccc caaactttga
    11461 gcggctcctt ctcgctcttc tcttcttcac ctttgatgaa aggaatgacg ttgtcgatca
    11521 tctccggcca cgactcgaac gtcttgcccg cgccgctgat cgcttgatac gtgcacaccg
    11581 cgatcttggt cggcttgaac gacttcagcg ggtgaatcgc gggcacgtag ctctggatcg
    11641 agcagttcgg cttgaccgag atgaagccgc gcttcgtgcc gagtcgcttg cgctgcgctt
    11701 cgatcaccgc gacgtggtcc gcgttgatct ccggaatcat catcggaacg tccggcgtcc
    11761 agcgatgtgc cgagttgttc gagacgacgg gcgtctccgc gcgcgcgtag tcttcttcga
    11821 gcttcgcggt ggcggccttg tccatgtcga ccgcgcacac gacgaagtcg cactgctcgg
    11881 cgatgcgcga gacttgcgat gcgtcgccga cggtgagcgc agcgaccttc gccggtacat
    11941 ccatcgcgag cgtccagcga ttcgcgacgg cgtcggcgta tgtcttgccc gcggaattcg
    12001 cgctggcggc gaccgccgaa acttcgaacc acggatgatc cgcgagcagc gaaacgaacc
    12061 tctggccgac catgccggtc gcgccgagga cgcctacctt gagcttgttt gccattgcac
    12121 tctgcaatct agcaaatccc tgataatgat cacgcgccgt gcctctgctc gatggactca
    12181 aagtcgttca ctcctcgatc catggctatg gcgtcatcac gacgcggcct ttcaaggcgg
    12241 gagagctcgt ctgctacggc gacggagtcc tctataaaga ggacgaggat ttcgacgaca
    12301 cgtacgcgct cgtgctgccc ggcgaagact cgggcctcgg tgagcagttc ttctgggacc
    12361 tcgccgatca gacgcggtgg ttcaatcact cgtgcgatcc gaacagcgag gtccgctcga
    12421 agtgggacca cgaggcgaac acgtgccgcg cgtggtgggt cgcgcttcgc gatatccccg
    12481 tcggtgagga gatcacgtac gactacgcgt tcgtcgccga ggttgccgag ccctgcgcgt
    12541 gcggcgcgaa gacctgccgc gggctcatcg tcgatccgga tccggcggag ctcgcgaagc
    12601 tgcccgagca cctgcgccag ctgctgcgcc cgcaggctgc gtagctgcaa tgagcacgcg
    12661 gatcggaatc ctgaccggcg gcggcgattg ccccgggctc aacggcgtca tccgcgcggt
    12721 gacactccat gcgcgcaaca cgttcggctg ggaggtcgtc gggattcgca acggcttcga
    12781 aggcctctac gaagagaact acgtcgacct ccacgtctcg acggtgcagc acctcctcgg
    12841 ccgcggcggc accatgctcg gctcgagcaa tcgcgccaac ccattcgcgt atccggtgaa
    12901 gctgcccgat ggccgcgtcg agatccacga cgtctcgaag cgctgcctga cgaacctcga
    12961 gaagctcgac ctccacgggc tcattgtcgt cggtggcgac ggaacgatgt cgttcgcgca
    13021 gaagttcatc gattgcggga tgcctcggat cgtcggcgtg cccaagacga tcgacaatga
    13081 cctcgaagcg acggactaca cggttggctt ccagaccgcg gtcgagattg ttgtcgaggc
    13141 gctcgagcgg ctccacacga cggcggagag ccacgagcgc atcatgatcg tcgaggtcat
    13201 gggccgctac gcgggctgga tcgcgctgac cgccggtctc gcaggtggcg cccacgtcgt
    13261 gctgattccg gagatcgatt acgaccccga gcgcgttatt gccgcattca accggcggca
    13321 tggtcgcggc gtcagccact cgatcgtcgt catctccgag ggcgcgaaac cgcgaggcgg
    13381 cagcctatcg gtcgtcacgg ccggcgaccc gacgaagcag gagaggctcg gcggcgccgc
    13441 ccaacggctc acggactacc tctgcaagga gaccgagtac gaggttcgct cgacggtgct
    13501 cggtcacgtg cagcggggtg gtgcgccgtg tgcgttcgat cgcgagctcg ccaccagatt
    13561 cggcgtccgc gccgtggagc tcatcgccga gggcaagttc ggccacgtcg ccgcgctcga
    13621 agccggaaag ctcgtcgcga agcccatcgg aacggccgtg aagaagctca agctcgtcga
    13681 tccgaatggc gagctcgtcc ggacggccaa gggcattggc atcgagctgg gtgggtgata
    13741 accctctcgg ccatgcgccg cgtcgcgtgg ctcctcattc tcctggccgc ctgcagctcg
    13801 aagcccagga gaggtgcggc tctcgaccag ccgggctcgt gtctcgcgtt gccgcctggg
    13861 ctgcgctttc atctcgagcc cgatccgcgt ggtgatggcc tctattggat ggaggcggtc
    13921 gagtacttcg actacctctc gggactgcgc tcgttcgaga acctcatccg ctacgatctg
    13981 cgcacgcgca ccacgacggt gatcaccgac cacgtgatca agccgctgct gttctccgac
    14041 ggcgatgtct acgtgcggcg cagcttcggc gacaagaaag agttcgtcca catcgacaag
    14101 ggcaagcgcg tgcacacgct gacgcccgac tatctcgacg tcgacgacgt actgcagctc
    14161 gacgagacga cgttcgcgct cctcgcggat ggcgacggcg cacgcgcggt ctacatcctc
    14221 gccaccgacc gcccgcgtcc gcgttacctc gcggaggcta atgcgctcgt cggccatgac
    14281 ggcacgcacg tcttgctgca gaagggcgcc gagtacctgt ctgtcgacgt tgcgacgggc
    14341 gccgcgaccc cgttcaagaa cgtgccaggc gctacggtgc tgggcccacg cgcattcctc
    14401 gtcgagaacg accagatcca cgtccaccac ctcatcaaag gcgatgacta cgtcgcggtc
    14461 gacaagaaga atcagtggaa gctcgtcttc cagcgcgact cgatgctcgt gcggactgca
    14521 ccgaaggacg atcgctccga ggcgttcctc atcgaagacg gcaaggcgac gccgctgccc
    14581 agcgtcagcg gtggcacgtc gatctacggc acggcgaagc tcggcgaccg gctgtgggcc
    14641 ctcatcggtc acaacacagc aaactcgatt ctcgatcctg ccgacacggt gacggagtcc
    14701 gaggtgtgcg cgctgcccgc gggcgggccg gtgatcttca agtcgcgcat cgtgccgggc
    14761 cgctttctcg cgaagtccga caagctgatg ggtgcgctga tcgccgcagc gcccggtgct
    14821 ggcgtccaga tcgcgcagga ctacaactat ccggtcgcgg tctcggtaac gatgcagacg
    14881 gaatacgcgc ggcgagactt tctgaagatg cgcgcgcgcg tgtccgagat ccacgaagca
    14941 gcgactcggc tcctcgaaga tccggacgtg cgcaccgagg tcacctgggc cgacggttgg
    15001 atcgcgatca aagcatggcg gcgcgaccgg ctgcgcgagg cgatatcggt cggcatgggc
    15061 gacgcgttgc tgtcgaaccc gctcgacgtc gacgtgatcg tcaccgatct cgtcgatgcg
    15121 catgtcgaca atggcttcga gtgctccggc tcgctcacga acacgcacgc cgagaagctc
    15181 gagaatctgg agctgcgctg cggcaccgac cgcatgcaca cgatccatgt cgcggcgatc
    15241 ggaccgcacg agaccgtcaa gttctcgaag gtctggccgc aaggcgacga ggacacctgg
    15301 atctcgccgc tgttcgagat cacgcgcaac ggcgagctca tgcagatgat cgacgactat
    15361 cacgagaagc agaccgagcg tctcctcgat ctgtgcgagc gccagtacgc ggcctcgaag
    15421 ttcacactgc tcacgcacac gatcgaccgc gatatcgcgc tgtcattctg ggctccggcg
    15481 gagtttgtcg actacaccga ggcagagcag ctgacgatcg tgcgcgacct ctatcggcgc
    15541 ttccgcgaag tggcgaagat ctacgacctg cctacgacga cgccgatgac gatcacgatc
    15601 tcggtccaca tgcacaccga gacctacgac tacgacggca ccgagctgac gaagcgctag
    15661 cctcggcgca tactcgcgcc atgtccgatt tggttcagcg cgacgaggtg cggccggagg
    15721 tcaacgcgtg ggatgcgccg ctccctgatc cgaacattca agacgcgcgc atcgctcaca
    15781 cgttcgcacc gctcgctggc gtacccgtcg tcggccgcgt cgaagcggca tatcgccagc
    15841 gcttccggct gaagtcctgg caatacatga cggcggtcac cgacgagctg ttcatcgcgt
    15901 ttgtcgtcgg cacggcgggc ttcgcgagca atggcttcgt ctatgccgcc gagctcgcga
    15961 ccggacgcgt ccacaagaag ttcgccatca cgccgctcat ggtcggcacc gagctcgcgc
    16021 catcgagcac gcaaggcatg catcggttcg ccacgcgcgg gctcggcgtg acgatcgaga
    16081 atcacggccg agcgttcgac gcgcgcgtgg atgcgaagac ggatggcggc ggcaagctgg
    16141 tcgccgacct cgcgttctcg agccgcaaga atgacgagca cctcgcgatc tgcgtgccgc
    16201 tccctggcaa acgctggaac tacacgcaca agtttgccgc gttcgacgtg accggctcgg
    16261 tgacgatcga cgatcgccgc atcgacttcg cgcccgggcg ttcgttcggc acgatggact
    16321 tcacgaagat gtacgcgctg cgccacgcgg tatggcgatg gatcgcgcta tgcggccgca
    16381 cgaaagaggg acgcatcgtc gggctcaacc tcgtcgatcc gacgcccgat gcgccgatca
    16441 gcgaaaacgc gctctgggtc gacgggaaac gccaggcgct gacaaacgtc cgcataggcg
    16501 cagatgacgc gcaggccgat ggcgtctcga tccaattgcg cgaggtcgcg caggtcgaac
    16561 agaagctcga cgtgcccctc gttcgtcatc gcttgcgcca cgtgatcgga tcgttctccg
    16621 gaagtgtaac gacccaaacg ggtgagaagc tcgatgtaaa agacgtcgtc ggaatttacg
    16681 aggactacga cacgtggtgg tgatgttgct cgcacgttac aggttgcatt tctcgacgag
    16741 gattgcgcac tgaagtgcgc atgagggtcc gaccaatgtg gatcccttca ttcgcttctt
    16801 gcaccacgca cactcgatct gtgtatggtg agcccgctct gagtctgagt cggagcaggg
    16861 tttcacgtgt tcggggttca gcgcatcggt cgatacgaag tcttgagcca cttggcgtcg
    16921 ggagggatgg ggcaggtgta cctggctcgc gcgaccggcc ttggtggctt cgagaggcaa
    16981 gtcgtcgtca agacgctcga cctcagcatc accgatgacg acgactcgtt cgtgacgatg
    17041 ttcctcgacg aggctcgcct cgttggagcg ctgcatcatc agtacatcgc gccggtctac
    17101 gaagtcggct gtgacgaaga gggtcgctac ttcctcgtca tggactacgt gcacggcgag
    17161 accgccgagg ccgtgttccg cgatgcgaac gagcgcaagc acgccatccc gctctcgttc
    17221 gcgttgaccg ttgtctcgtg cgtcgcgtcc gcgctcgact acgcgcacag cctctgcgct
    17281 gcggacggca caccgctcga gatcgtgcat cgcgacatct cgctgtcgaa cgtgatgatc
    17341 ggctacgacg gcggcgtcaa gctgatcgat ttcggcatcg cgaaggcgtc gaaccgaacc
    17401 acgaagacgc aggtcggcac gctcaaaggc aagatcggct acctcgcgcc cgagcagatc
    17461 ctgcgcaagc acgtcgatca ccgcgccgac atcttctcgc ttggcattgt tctctacgag
    17521 ctgacgaccc aagcgcgtgc gttcaaggcg ggctcggacc tgatcacgct cgaacgcatc
    17581 acgaagggcg acatcgtcgc gccctcgaaa atcgtgccgg actatccgcg cgagctcgaa
    17641 gcgatcgtga tgcgcgcgct cgaggtggac ccagacgagc ggttccaaga tgccggcgag
    17701 atggggcgcg cgctcgaatc actcgccgag cacaaggggc tgcgcctcgg ccacggcccc
    17761 gtcatcgatg tgatgcgcgc gctgttcgcc gagaagcgcc cgcgccgccg cctcgcgcgc
    17821 tcgagcgacg agctccgcaa gcagaaccaa gctatcgaag cagatgcgga tgaggatacg
    17881 gatgtccgta gctcgcatcc gcgctcgcac aagccgacgc tgccgatgaa accgtcgccc
    17941 acgtcgatgc cagcggtggt tccaccgtcg ggcgccgacg acgatcgtgc caccgtgccg
    18001 atcatggctc gcggcacacg cgacggtatg gtgccgatgg atcatccgtc gcgcctaccg
    18061 acgccgctgc cgatcgagga gcaatcgacg ggcgtcgtcg atcccaacct cgaggtccgc
    18121 ttcgcgcacg cgaagattcc actcgaggac cggccgacgc caatcgccga tgcgtctctg
    18181 atcgatcccc ccacgcatcg cgacgcgaag ccgctcgtga tcgacgacac ccaaccgcgc
    18241 gagcgattga cgccgccgga gcgtccctcg ggcaatggca tcgcggttcc gcgtacgctc
    18301 acggggcaac cgccgccacc gacgcgccgc gcgacgtcgc agccgccgcc gattccggcc
    18361 ggcatccctc cgttcccgcg cagcgtcaca gggccgccgt tgccgcctca cctcgaggac
    18421 gcgccgacgg agatcgcggc gatgcgcaca gagcgcgagg acccgaccga cgtctcgggt
    18481 gtcgagccgc tgccgcgcac gaccacgcag ctcacgccga ctgcgcagct cgccctgcgc
    18541 cagaagtggg gcccggcgta cgtcgcaacc gccgtcgtca atcgctaccg caagaagtcg
    18601 cgcgatacgg cgacgcagac gtggttcatc ttgctcggac tgttcggcgt cgccgtgatc
    18661 accgcgatgc tcgtcgcgct gctctagcga aagaagcgat gtagctcgca gccagcggcg
    18721 tgcgctcggc tctcgagctc ttcacgcgcg ctcgtacagc tctccgcaat cttcgaacgc
    18781 tggtcgggcg gcacgagctt ccacgcctct tccgattgcc ggaatccgtc gagcatcgtg
    18841 gcacccgtat ccgcggtgac gctgtcgcac cagaagtagc tgtacagcga gtcgccgtac
    18901 gcgtcgcact cgtcgatgcc ggtcttggtc cagtgttgcg gctcgaccgg actgccgaaa
    18961 tcgatcttgc ccatcgtgtg ctcgagctcg tcggtgctca ggctcccgac ctgttcgggc
    19021 gggtcattcg cggacagcgc gatcgcacct gcggtgccgc ccgcgagtgt caccgcgatc
    19081 cctgcagcga ggtagttacg ccgccggacg cctctggcgc cgacctcgca gtccgcgagc
    19141 aggatcgtgc cgtccttgcg ttggtagtaa cgcgcgcaca ggtcgcccgc gcgctcgcga
    19201 atcagcgatt cggcttgctc gcgtgtgagc gctgagagat cgaacacgtc cttgtcgcat
    19261 ttgccgcagt gacggactcg ctcgtcgccg ctcatcgcat cccagcgctc ggtgcacggt
    19321 gacgcgatgc ggagattcgg cagcaccggc aggcgagtgc gcgccttcgc ctcctcgagg
    19381 atctgcgccg ttgcgtcgcg ttcacgcgtt gcgctgtcga cggcggcatc gagtgcgacc
    19441 ttgcgcgcct gcagtgcttc gaggtcgttg cggtaggtca cgaatagata gacacgcgcc
    19501 accctacgat cgattgcaga aatcgacagg ccgccgcaga tcgcgcattt cgcgctgcgt
    19561 cagctcgtgc cggcgtcggc ggtcatcgtt tggccgtcgc gcacatagct cgatccgggg
    19621 atggccttcg ccgttccctt accgacggcc gccagctcgg cgagacccgc gtacgtcttg
    19681 gaccgcgaca gcgagatgcc cgcgatcgac acgctcatga tcgggaactt acgctgcacg
    19741 ccgaagcggt ccttcgtctc gatgtagcca cgctgccgat cgttcggatc gtagtagagg
    19801 cggatcaagt gatcgaagcg atcgcagatc gcgcggcaca cttcgtc
//
LOCUS       DATLBZ010000236         7744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6086512, whole genome shotgun sequence.
ACCESSION   DATLBZ010000236 DATLBZ010000000
VERSION     DATLBZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6086512"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5616)
                     /locus_tag="VMZ53_12680"
     CDS             complement(<1..5616)
                     /locus_tag="VMZ53_12680"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HUS29363.1"
                     /translation="MKRALVLLVVAACGSKSDTKKQPQPPLSTKLAQQAKDLPDGIDI
                     RLSDGKQGPPPFDHNKLAAAKKLGDADVQQLLARAKPLETDPDDSKDFALRPMSQPPP
                     RTGNTIKDTFPAAVTMPPPPTAANANKDLEVLRFMPEGEIPYAPELSVTFNQPMVAVT
                     SQDDAAKVQPVKLDPQPKGKWRWIGTRTILFDPDPRFPQSTTYKITIPEGTKSATGQT
                     LKKAVSFSFETPAPKLVAQFPYSGAPQHLDVPMFVMFDQRIDPQSVLSKIKVTVPGPA
                     KQQQQQPQQKPNPLKPQIKKQDTPDPWAAGATPSGSHTVGLKLLSDAEIQKNNTLKNL
                     VEGASKDENNKHKWLAFRATEEFPPNASITVEIPEGTPSAEGPNKTKQSQSFSFSTYP
                     PLQVISTSCDYNRDQCPPSVPLAMYFNNPIDADKFDEKLVTFSPEIEHSQIVQSGSMV
                     AVYGFTKSRTTYKMTIGGGIIDEFGQKLGHDETRTFTIGDAAPTFYGPSGMVVVDPQG
                     QKPTLDFFSTNYESLKVRIYKVTPGNLRGYYTYLENLWNKDHPPTPPGTKVFDQLVTT
                     GGKQNELVETHVDLAVAMNKAGLGHAIVIVEPHPWNQEWDPPRMISWVQSTKLAVDAH
                     VDGDHLVAFTTELSSGKPLSGIDVELAPTGTHGTSDDKGLASIPLPSGQQSTNVLYAR
                     RGDDTALLLENEGYWSGYGSWYRQGRDKQLAWYVVDDRKLYKPGEEVSLKGWLRTIDY
                     GKGGDVGGQPDAIKTITYTVTDAVGNQIGKGSMQVSPVGGFDTKFTLPKTPNLGYANV
                     SMQTVGYPGYYSHSFQLQEFRRPEFEVSAQASTGPFSIGEGGDITVSAKYFAGGALPG
                     AEVNWYVNASQTSFTPPNRDDFTFGQWTPWWGYRSWYDDTSNDYKPQPSWNWHGKTDA
                     LGEHVLHMDFLSAKPSLPYSVTANASVMDVNRQQWNASTSLIVHPSSRYVGVKVQRPY
                     VEKGTPFDLEVIGVDIDGKATPGAKIEVKTVRLDYEYKRGRYQTKEVDPQTCMVTAAK
                     DPSPCEFKTDKGGTYQVTATIVDDKGRANTTKLTYWVSGGDNPPARDVAMEQVQLIPD
                     KKEYSAGNTAEILVQAPFYPAEGIVTWRRSGIVKTERISITGPTASLKVPITEDLVPN
                     LYVQVDLVGMQLRTDDAGVPDPKLPKRPAYAAGSIDLPIPPKQRTLTVDVTPSAEKLG
                     PGEETSLALVVKDAQGKPVADAEAAVIVVDEAILSLTGYQFPNPVDSFYPQRGPDTRD
                     YYQRAYVKLARPDLTKAQGNMPMPGGGGSFRDRGGVEEGDFEMAKQSALYDEKPMDMP
                     PVAAAAPAAEPMMKKAESKSTFSPKPAPPAKTMTSTGSRIAVGGKDKADAISLARNQG
                     VLGSVATTPTTQPQPNTPVTVRTNFNPLAAFSPTVKTDASGKATVTVKMPDNLTRYRV
                     VAIAVAGNKQYGKGESAITARLPLMVRPSPPRFLNFGDSFQMPVVVQNQTDKPLTVRI
                     AARTANMSLTDGAGREFMVPPNDRVEVQLPAAAEMAGTARFQVIAAAGDASDAADLEL
                     PVWTPATTEAFATYGVIDEGAMAQPVALPGKVWPQFGGLEVTTASTNLQSLTDAVIYL
                     VKYPFDCAEQRSSRILALASLRDVLAAFKSKDLPSTAAMEHSVDIDVEHLTQMQNWDG
                     GFVYWERGHPSEPYLSVFVMQALYKAKSHGYTVPAQMTTRGLQFLRNIEGYYPYWYDA
                     DIRHAISAYALYVRKQHGDLDIAKAKALYAELGKLDTASMETLGWLLSTFAGNAGAAK
                     ERQEIVRYAINHVSETAGAANFTTGYGDGKYLLLASDRRTDGVMLEALILEDPKNDLI
                     PKIVTGLLAHRTKGHWLNTQENAFVLTALE"
     gene            complement(5626..>7744)
                     /locus_tag="VMZ53_12685"
     CDS             complement(5626..>7744)
                     /locus_tag="VMZ53_12685"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HUS29364.1"
                     /translation="SPAVKTDAQGKAVVELKMPDNLTRYRIVAIVASGENHFGKGENA
                     VTARLPLMVRPSPPRFLNFGDTFKLPVVVQNQTDSPMTVKVAVRATNAAITDGAAREV
                     TVPPNDRVEVQFPAAAEMAGTARFQIVGASGANSDASELALPVWTPATTEAFATYGVI
                     DDGATRQPVALPGQVIKEYGGLEITTASTNLQSLTDAFLYLVHYPFECAEQRSARILG
                     IAALRDVLEAFQTKDMPTRAALEKSVAEDIEHLSQMQNYDGGFAFWDRGYPSEPYLTV
                     FVTNALTHAKAKGFKVPQQMLDRAKPYLQQIEQHYPWYYSKEVRWAISAYALYTRKQM
                     GDLDIKKGQALYKEAKIGDPKTTASMETTGWLLGTFAGNKDAEVERKAIIKYALNHVS
                     ERAGVANFTTGYADGNYLLLSSDRRVDGVMLESLIQEEPKNDLIPKIVTGLLAHRKAG
                     RWLNTQENSFILLALDLYFQTYEKVTPDFIARVWLGKDYAGDHPFKGRSTDYFDIDIP
                     MAFVATHDKQDLIIQKDGKGRLYYRVGMTYAPASLKLEPADYGFVVQRVYEGADDPKD
                     VTRAADGSWHIKAGARVRVKLTMVNENRRYHVALVDPIPAGLETMNPALAVTGPIPQD
                     PNEQKSRGRYWWWYGPWYEHQNMRDERNEAFAALLWEGVHEYEYVARATTPGNFVVPP
                     AKAEEMYMPETFGRSGSDRVIVE"
BASE COUNT         1180 a         2418 c         2527 g         1619 t
ORIGIN      
        1 ctcgagcgcg gtgagcacga acgcgttctc ttgcgtgttg agccagtggc ctttcgtgcg
       61 gtgagcgagc aggcccgtga cgatcttcgg aatgaggtcg ttcttggggt cttcgaggat
      121 cagcgcttcg agcatcacgc cgtctgtccg gcgatcggac gctaggagga ggtacttgcc
      181 gtcgccgtag ccggtcgtga agttggccgc gcccgccgtc tcgctaacgt ggttgatcgc
      241 gtagcgcacg atctcttggc gctctttggc tgcacctgcg ttgcccgcga acgtcgacag
      301 cagccagccg agcgtctcca tcgacgcggt gtcgagcttg ccgagctcgg cgtagagcgc
      361 cttggctttc gcgatgtcga gatcgccgtg ctgcttgcga acgtagagtg cgtacgcgct
      421 gatcgcgtgg cgaatgtctg cgtcgtacca gtacgggtag tagccctcga tgttgcggag
      481 gaactgcagg ccgcgcgtcg tcatctgagc gggcacggtg tagccgtgcg acttcgcctt
      541 gtagagcgcc tgcatcacga atacggagag atacggctcg cttggatgac cacgctccca
      601 gtacacgaag ccgccgtccc agttctgcat ctgcgtgagg tgctcgacgt cgatgtcgac
      661 cgagtgctcc atcgcggcgg tgctaggcag atccttgctc ttgaatgcag cgaggacgtc
      721 gcgcagcgac gcgagtgcga ggatgcgaga gctgcgctgc tcggcgcagt cgaatggata
      781 tttgacgagg tagatcacgg cgtcggtgag cgactgcaga ttcgtcgacg cggtcgtcac
      841 ttcgaggcca ccgaattgcg gccagacctt gcccggtagc gcgaccggtt gcgccatcgc
      901 gccttcatcg atgacaccgt aggtcgcgaa tgcttcggtc gttgccggcg tccacaccgg
      961 cagctcgagg tcggcagcgt cgctcgcgtc gccagcggcg gcgatcactt ggaagcgagc
     1021 cgtgcccgcc atctcggctg cggcggggag ctgcacctcg acgcgatcgt ttggcggcac
     1081 catgaactcg cggcctgcgc cgtcggtgag cgacatgttc gcggtgcgcg ctgcgatgcg
     1141 aaccgtcagc ggtttgtccg tctggttctg cacgacgacc ggcatctgga acgagtcgcc
     1201 gaagttgagg aagcggggcg gcgacggacg aaccatcagc ggcaagcgcg cggtgatcgc
     1261 actctcgcct ttgccgtact gcttgttgcc cgcgacggcg atcgcgacga cgcgataacg
     1321 cgtgaggttg tcgggcatct tcacggtgac cgtcgccttg ccactcgcgt cggtcttgac
     1381 cgtcggcgag aacgcagcga gcgggttgaa gttcgtgcgc accgtgacgg gcgtgttcgg
     1441 ctgcggctgc gtcgttggcg tcgttgcgac gctaccgagc acaccttggt tgcgcgcgag
     1501 cgagatcgcg tctgctttgt ccttgccacc aaccgcgatg cgactgccgg tcgatgtcat
     1561 cgtcttcgcc ggtggagctg gcttcggtga gaatgtcgac ttcgattccg ccttcttcat
     1621 catcggctct gctgcaggtg cagcggccgc gacgggcggc atgtccatcg gcttctcgtc
     1681 atatagcgcc gactgcttcg ccatctcgaa gtcaccttct tcgacgccac cgcggtcgcg
     1741 gaagctcccg ccgccgccgg gcatcggcat gttgccttgc gccttcgtca gatccgggcg
     1801 cgcgagcttc acgtacgcac gctggtagta gtcgcgcgta tcggggccgc gctgcggata
     1861 gaaggagtcg accggattcg ggaactgata gccggtgagc gagaggatcg cttcatcgac
     1921 gacgatcacc gccgcctctg catcggcgac cggcttgccc tgtgcgtctt tgacaacgag
     1981 cgcgagcgac gtctcttcgc cgggcccgag cttctcggcg gagggcgtca cgtcgacggt
     2041 gagcgtgcgc tgcttcggcg gaatcggcag atcgatgctg cctgccgcat acgcaggacg
     2101 cttcggcagc ttcggatcgg gcacgcccgc gtcgtcggtg cgcagctgca tgccgacgag
     2161 atcgacttgc acgtagaggt tcggcacgag gtcctcggtg atcggaacct tgagcgacgc
     2221 cgtcgggccc gtaatcgaga tgcgctcggt tttcacgatg ccgctgcggc gccacgtgac
     2281 gatgccttcc gccggataga acggtgcctg cacgaggatc tcggccgtgt tgcccgccga
     2341 gtactccttc ttgtcgggga tgagctgcac ttgctccatc gcgacatcgc gcgccggcgg
     2401 gttgtcacca ccggagaccc aatacgtgag cttcgtggtg ttcgcgcggc ctttgtcgtc
     2461 gacgatcgtc gccgtcacct gatacgtgcc gcccttatcg gtcttgaact cgcagggcga
     2521 cggatctttc gcggccgtca ccatgcacgt ctgcggatcg acttccttcg tctgatagcg
     2581 accgcgcttg tactcgtaat cgagacgcac ggtcttcact tcgatcttcg cgccgggcgt
     2641 tgccttgcca tcgatatcga cgccgatgac ttcgagatcg aacggcgtgc ccttctcgac
     2701 gtacggccgc tggaccttca cgccgacata gcgcgacgac ggatgcacga tgagcgacgt
     2761 cgaggcgttc cactgctggc ggttgacgtc catgacgctc gcattcgcgg tgaccgagta
     2821 cggcagcgac ggcttcgccg agaggaagtc catgtggagc acgtgctcgc cgagcgcatc
     2881 ggtcttgccg tgccagttcc agctcggctg cggcttgtag tcgttcgacg tgtcgtcgta
     2941 ccagctgcgg tagccccacc acggcgtcca ctgaccgaac gtgaagtcgt cgcggttcgg
     3001 cggcgtgaac gacgtctgcg acgcgttgac gtaccagttc acttcggcgc caggcagcgc
     3061 gccgccggcg aagtacttcg cgctgacggt gatgtcgccg ccctcgccga tcgagaacgg
     3121 tcccgtcgac gcttgtgccg agacttcgaa ctcggggcga cggaactctt ggagctggaa
     3181 cgaatgcgag tagtagccgg gatagccgac cgtctgcatc gagacattgg cgtagccgag
     3241 gttcggcgtc ttcggcagcg tgaacttcgt gtcgaagcca ccgactgggc tcacctgcat
     3301 cgagcctttg ccgatctgat tgccgaccgc gtccgtgacc gtatacgtga tcgtcttgat
     3361 cgcatcgggc tggccgccga cgtcgccgcc cttgccgtaa tcgatcgtgc gcagccaacc
     3421 cttgagcgag acttcttcgc cgggcttgta gagcttgcga tcgtcgacga cgtaccaggc
     3481 gagctgtttg tcgcgacctt ggcgatacca gctgccgtac ccactccagt agccctcgtt
     3541 ctcgaggagc agcgcggtgt catcgccgcg gcgcgcgtag agcacgttcg tcgactgctg
     3601 ccccgaagga agcggaatcg atgcgagacc cttgtcgtcg ctcgtcccgt gcgtgcccgt
     3661 tggcgcgagc tcgacatcga tgcccgatag cggcttgcca ctcgagagct ccgtcgtgaa
     3721 cgcgacgagg tgatcgccat cgacgtgcgc gtcgaccgcg agcttcgtcg actgtaccca
     3781 cgagatcatg cgcggtggat cccactcctg attccacgga tgcggctcga cgatcacgat
     3841 cgcgtgaccg aggccggctt tgttcattgc gacggccaga tcgacgtgcg tctcgacgag
     3901 ctcgttctgc ttgccgcccg tggtgacgag ctggtcgaag actttcgtgc ccggcggcgt
     3961 cggcgggtga tctttgttcc agagattttc gagatacgtg tagtagccgc gcaggttgcc
     4021 cggcgtgacc ttgtagatgc ggaccttgag cgactcgtag ttcgtcgaga agaagtcgag
     4081 cgtcggcttc tggccctgcg gatcgacaac gaccatgccg ctcggtccgt agaacgtcgg
     4141 cgcggcgtcg ccgatcgtga acgtgcgcgt ctcgtcgtgg ccgagcttct gcccgaactc
     4201 gtcgatgatg ccgccgccga tcgtcatctt gtacgtcgtg cgcgacttcg tgaagccgta
     4261 caccgcgacc atcgagccgc tctgcacgat ctgcgagtgc tcgatctctg gcgagaacgt
     4321 cacgagcttc tcgtcgaact tgtcggcgtc gatcggattg ttgaagtaca tcgcgagcgg
     4381 cacgctcggc gggcactgat cgcggttgta gtcgcacgag gtcgagatga cctgcagcgg
     4441 tggataggtg ctgaacgaga agctctgcga ctgcttcgtc ttgttcggac cctcggcaga
     4501 cggcgtgcct tccggaatct cgacggtgat cgacgcgttc ggcgggaact cttcggtcgc
     4561 gcggaacgcg agccacttgt gcttgttgtt ctcgtccttg ctcgcgccct cgacgaggtt
     4621 cttcagcgtg ttgttcttct ggatctcggc gtcgctgagg agcttcagcc cgacggtgtg
     4681 cgagcccgac ggcgttgcgc ctgccgccca cggatctggg gtgtcctgct tcttgatctg
     4741 cggcttcagc ggatttggct tctgctgcgg ctgttgctgc tgctgcttcg cgggccccgg
     4801 cacggtgacc ttgatcttcg acagcaccga ctgcggatcg atccgctggt cgaacatcac
     4861 gaacatcggc acgtcgaggt gctgcggtgc gccgctatat gggaactgcg cgacgagctt
     4921 tggcgccggc gtctcgaacg agaagctgac ggccttcttc aacgtctgtc ccgtcgcgct
     4981 cttcgtgccc tcggggatcg tgatcttgta cgtcgtcgac tgcgggaacc gcggatctgg
     5041 atcgaacagg atcgtgcgcg taccgatcca gcgccacttg cccttcggct gcggatcgag
     5101 cttcaccggc tggaccttcg ctgcatcgtc ttgcgacgtg accgcgacca tcggttggtt
     5161 gaacgtcacc gacagctccg gtgcgtacgg aatctcgccc tcgggcatga agcgaaggac
     5221 ctcgagatcc ttgttcgcat tcgcggccgt cggcggaggc ggcatcgtca ccgctgcagg
     5281 gaacgtgtcc ttgatcgtgt tgcccgtgcg aggcggcggt tgcgacatcg gccgcagcgc
     5341 gaagtccttc gagtcatcgg gatccgtctc gagcggcttc gcgcgcgcga gcagctgctg
     5401 cacgtcggca tcgccgagct tcttcgccgc ggcgagcttg ttgtgatcga acggcggcgg
     5461 accctgtttt ccgtcggaga gccggatgtc gatgccgtcc ggcaagtctt tcgcttgctg
     5521 ggccagcttc gtcgacagcg gtggttgcgg ctgcttcttc gtatcggact tgctgccgca
     5581 cgcagctact accagaagga ctagtgcacg tttcacgcga gctccttatt cgacgatgac
     5641 tcggtcacta ccgctgcgcc cgaacgtctc gggcatgtac atctcttcgg cctttgcagg
     5701 cgggacgacg aagttgcctg gcgtcgtcgc gcgcgcgacg tactcgtatt cgtggacgcc
     5761 ttcccagagc agcgccgcga atgcttcgtt gcgttcgtcg cgcatgttct ggtgctcgta
     5821 ccaaggtccg taccaccacc agtaacgacc gcgcgatttc tgctcgttcg gatcctgcgg
     5881 aatcggaccg gtgaccgcga gcgccggatt catcgtctcg agccccgcag gaatcggatc
     5941 gacgagcgcc acgtggtagc gccggttctc gttgaccatg gtcagtttga ccctcacgcg
     6001 cgcgccggct ttgatatgcc agctgccatc ggccgcgcgc gtgacgtcct tcggatcgtc
     6061 ggcgccctcg taaacacgct gaaccacgaa gccatagtcc gcgggctcga gcttgaggct
     6121 cgcgggagcg taggtcatgc cgacgcggta gtagaggcgg cctttgccgt ccttctggat
     6181 gatgaggtcc tgcttgtcgt gcgtcgcgac gaacgccatc gggatgtcga tgtcgaagta
     6241 gtccgtcgag cggcctttga acggatggtc gcccgcgtag tccttgccga gccacacacg
     6301 cgcgatgaag tcgggcgtga ccttctcgta cgtctggaaa tacagatcga gcgcgagcag
     6361 gatgaagctg ttctcctgcg tgttgagcca acggccggct ttgcgatgcg cgagtaggcc
     6421 cgtcacgatc ttcgggatca gatcgttctt cggctcttct tggatgagcg actcgagcat
     6481 cacaccatcg acgcggcgat cgctcgacag cagcaagtag ttgccgtcgg cgtagccggt
     6541 cgtgaagttc gcgacgcccg cacgctcgct cacgtgattg agcgcgtact tgatgatcgc
     6601 cttgcgctcg acctctgcat ccttgttccc ggcgaacgtg ccgaggagcc agccggtcgt
     6661 ctccatcgac gcggtcgtct tcggatcgcc gatcttcgct tctttataga gagcctgccc
     6721 cttcttgata tcgaggtcgc ccatctgctt gcgcgtgtag agcgcatacg cgctgatcgc
     6781 ccagcggact tccttgctgt agtaccaggg atagtgctgc tcgatctgct gcagatacgg
     6841 cttggcgcga tcgagcatct gctgcggaac cttgaagccc ttcgccttcg cgtgcgtgag
     6901 cgcgttcgtg acgaagacgg tgaggtatgg ctccgacgga taaccgcgat cccagaacgc
     6961 gaagccgccg tcgtagttct gcatctgtga cagatgctcg atgtcctcgg cgacggactt
     7021 ctcgagcgcc gcgcgcgtgg gcatgtcctt tgtctggaat gcttcgagca cgtcgcgcag
     7081 cgctgcgatg cctaggatgc gcgcgctgcg ttgctcggcg cactcgaacg ggtagtgcac
     7141 gagatagagg aacgcgtcgg tgaggctctg tagatttgtc gacgcggtcg tgatctcgag
     7201 accgccgtac tccttgatga cttggcccgg cagcgcgacc ggttggcgcg tggcgccgtc
     7261 gtcgatcacg ccgtaggtcg cgaatgcttc cgtcgtcgcg ggtgtccaca cggggagcgc
     7321 gagctccgat gcatcgctgt tcgcgccact cgcaccgacg atctggaagc gcgcggtgcc
     7381 ggccatctcg gctgcagccg ggaactgcac ttcaacgcga tcgttcggcg gcacggtgac
     7441 ctcgcgggcc gcaccgtcgg tgatcgcagc gttcgtcgcg cgcaccgcga ctttcaccgt
     7501 catcggcgaa tcggtctggt tctgcacgac gacaggcagc ttgaacgtgt caccgaagtt
     7561 caagaaccgc ggcggcgatg ggcgcaccat gagtggcagc cgcgcggtga ccgcgttctc
     7621 gcctttgccg aagtggttct ctcccgaggc aacgatcgcg acgatgcgat aacgcgtcag
     7681 gttgtccggc attttgagct cgacgacggc cttgccttgc gcgtcggtct tcaccgcggg
     7741 cgag
//
LOCUS       DATLBZ010000237         2015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5470199, whole genome shotgun sequence.
ACCESSION   DATLBZ010000237 DATLBZ010000000
VERSION     DATLBZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5470199"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VMZ53_12690"
     CDS             complement(<1..536)
                     /locus_tag="VMZ53_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS29365.1"
                     /translation="MAEDERTRRWQRISSPAIGADPVHALRSAIAEIRNAPRDQEARR
                     QLRALAAEQGTWEQLALLLDDESRAAHKKPELAAAMFEELADVHENLDQVVEAISAME
                     NVVELQPEDLQHHDRLAWLYRKAGAGVKAAEAFERVAELARDDRARAALRAAGRLYRD
                     AGKSEQAVRVYRAIVKRRP"
     gene            573..>2015
                     /locus_tag="VMZ53_12695"
     CDS             573..>2015
                     /locus_tag="VMZ53_12695"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HUS29366.1"
                     /translation="MQLRRVVIGIGTSLVFAAPSHAGGLFVPGSGAVSTSRAGAAVAS
                     TDDGEALSINPSGLAKADGLKLTVAATLIKYYMSFARRGTSDPVGVVGDDRPYEGNPY
                     VVVENDPKPPLGIGTFQPLPVIVITSDLGDRVPGLTIGGGLYTPSGYPFRDMSQGYQF
                     QTDPNADYSVAPPPTRYDVMTQESALLLPSVAAAYRITPDLDVGVRLTAGNLKSKTQV
                     VVWGTPGNVEEDIKKDALFTADVKDGFIPAAGLGVTYRATPNIELGANWTSPVVVRAK
                     GTAKSVRGASVDMSRSIGNVPDSEAKCETGGTMTEQKACISLQLPMTATVGGRYKILD
                     EKDELLADVELDVNWENWGKTCDFDDASIVGSTCTAPGQYRIQIDAGLYTNNTYAQPI
                     NSTGGANVLNLGLKNTYGVRLGGSYHLPAGDNKVVLRGGVAYDTAAAKTGWLRANFDG
                     ASRITTALGGAYEVSGWQINAGFGYVFEGTN"
BASE COUNT          347 a          724 c          615 g          329 t
ORIGIN      
        1 ggtcgccgct tcacgatcgc gcgatacacg cgcacggctt gctcgctctt gccggcgtcg
       61 cgatagagcc ggcccgccgc gcgcagtgct gctcgcgctc gatcgtcacg cgccagctcc
      121 gccactcgct cgaacgcctc cgccgctttc accccggcgc ccgccttgcg atacagccac
      181 gcgaggcggt cgtgatgctg caaatcctcg ggctgcagct cgacgacatt ctccatcgcc
      241 gagatggcct cgacgacctg atcgaggttc tcgtgcacat ccgcgagctc ctcgaacatt
      301 gcggccgcga gctcgggctt cttgtgcgcc gcgcgcgatt cgtcgtcgag caggagcgcg
      361 agctgctccc acgtgccttg ctccgctgcg agcgcgcgca gctgccgcct cgcctcctga
      421 tcgcgtgggg cgttgcggat ctcggcgatc gcgctgcgca gcgcatggac cgggtccgcc
      481 ccgatggccg gcgacgatat ccgctgccag cgtctcgttc gttcgtcctc ggccacgact
      541 actcccaact ctagtgtaga ttcgcgacca gcatgcagct ccggcgggtt gttatcggca
      601 ttggcacatc cctggtcttt gcagctccgt ctcacgcagg cgggctcttc gtgcccggct
      661 cgggcgcggt gtcgacatcg cgcgcaggcg cggcagtcgc atcgaccgac gacggcgaag
      721 ctctctcgat caatccgtcg ggcctcgcaa aagccgacgg cctcaagctc accgtcgcag
      781 caacgctcat caagtactac atgagcttcg cgcgccgcgg cacgtcggac ccggtcggcg
      841 tcgtcggtga tgatcgcccg tacgaaggca acccgtacgt cgtcgtcgag aatgacccga
      901 agccgccgct cggcatcggc accttccagc cgctcccggt catcgtgatc acgagcgacc
      961 tcggcgatcg cgtcccgggt ctcacgatcg gtggcggtct ctacacgccg agtggctatc
     1021 cgttccgcga catgtcgcag ggctaccagt tccagaccga cccgaacgcc gactactccg
     1081 ttgcgccacc gccgactcgc tacgacgtga tgacgcaaga gagcgcgctg ctcctgccgt
     1141 cggtcgcggc cgcgtatcgc atcacgcccg acctcgacgt cggcgtccgt ctcaccgccg
     1201 gcaacctcaa gagcaagacg caagtcgtgg tctggggcac accgggcaac gtcgaagagg
     1261 acatcaagaa agatgctctc ttcaccgccg acgtcaaaga cggcttcatc cccgctgccg
     1321 gcctcggcgt cacgtatcgc gcgactccga acatcgagct cggcgccaac tggacgtcgc
     1381 ccgtggtcgt ccgcgcgaag ggcaccgcca agagcgttcg cggtgcgagc gtcgacatgt
     1441 ctcgctcgat cggcaacgtg cccgactccg aagcgaagtg cgagaccggc ggcacgatga
     1501 cagagcaaaa ggcctgcatc tcgctgcagc tgccgatgac cgccacggtc ggcggtcgct
     1561 acaagatcct agacgagaaa gacgagctgc tcgcggatgt cgagctcgac gtcaactggg
     1621 agaactgggg caagacgtgc gacttcgacg acgcgtcgat cgtcggctcg acgtgcaccg
     1681 cgcccggcca ataccgcatc cagatcgatg caggcctcta cacgaacaac acctacgccc
     1741 aaccgatcaa ctcgaccggc ggcgcgaacg tcttgaacct cggcctcaag aacacgtacg
     1801 gcgttcgtct cggcggcagc tatcacctgc ccgcgggcga caacaaggtc gtgctccgcg
     1861 gcggcgtcgc gtatgacact gcagccgcga agacaggctg gctgcgcgcg aacttcgacg
     1921 gtgcatcgcg catcaccacc gcactcggcg gcgcatacga agtgagcggc tggcagatca
     1981 acgccggctt cggctacgtg ttcgaaggca cgaac
//
LOCUS       DATLBZ010000238        10449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7578991, whole genome shotgun sequence.
ACCESSION   DATLBZ010000238 DATLBZ010000000
VERSION     DATLBZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7578991"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VMZ53_12700"
     CDS             complement(<1..437)
                     /locus_tag="VMZ53_12700"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HUS29367.1"
                     /translation="MRWDRSYRSSNIEDRRGEGFSGGGGGFGFGGGGGGFNILGLLGM
                     FGWKGILIGLVLMAVLAGGGTCLGGGQGCALGGGGSQPTSPTRSGGGTGGGPVQSSAE
                     EDDLVNFVGYVFDDVQNTWKKTFDNYEVTRIVLFRNGTQSACGT"
     gene            complement(572..1258)
                     /locus_tag="VMZ53_12705"
     CDS             complement(572..1258)
                     /locus_tag="VMZ53_12705"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HUS29368.1"
                     /translation="MRYALLVLILAGVAAGAGVTLRGQSTFRLHAASAETEAVPLVVV
                     LHGDHEDSRASLRRWQTAVEARGWELLALDCPRDRGCEDGSWYRWNGNPRWLRDRVHD
                     VTSKRAIDPTRIYLVGWSGGATYIGMHLQAWPDLFAAVVIHGGGVPPRNAHCIDRTFP
                     AYFLVGDQNPAHGAAVRLRDHFDRCGQEVKWDLVPGANHPREDAALTRAKADAILRWL
                     YERRRNDALS"
     gene            1476..3296
                     /locus_tag="VMZ53_12710"
     CDS             1476..3296
                     /locus_tag="VMZ53_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HUS29369.1"
                     /translation="MTTARASSQALVPKLTAEGECIHVQLFEDGQDKGPICIADAQAR
                     GLTVVDLTDQWTPTLFDPTDGQAPTFRARYLELAAEKSEGDSLEELTELYGVVPALSI
                     VHKRLADDQRHACHAAIDPSPILALDTTLSQGNKDTVQAGIVGRDWLEKELEKERARQ
                     KLPDIAALEADPKWHDRFIRYKKLDQTHTALLAAQQQLHCEGWMQDQDVDGTFTWRMG
                     MAVEMFQRRNFLMPTQRLDPETREALQLDSRELDFRLALRILRERVTAAAGLIEDGTA
                     GEGPQPVLGRQLDPPAMRAARGHKPMANAAPDLVDLATEAAAKQLGWTTPNDVRAFLD
                     RMGGSGVRVAIELPPLPAYHSTHMDITAEIDRGDIYYEDQPPPFKRILPHRPTLVLYA
                     MDGETKRPLVRWPTTVGGWADQRLKSGRIVQKWKESDVGPRVWKDLTAAPTWIPPKTT
                     PDKDLVKPTYPGWALKSEIMGPGPRAAYGMVLLEHVLPVKLKDGTERFDDNGIGTHGS
                     SSVTSIVGGNSHGCHRLYNQLAVRLGDFLLHHRNHVVKGETPVGFRRIVWHSGEAFRA
                     EIESRGFQYELTPPVPVNVLKGNILSKRKTPPKNSAPARP"
     gene            complement(3301..3648)
                     /locus_tag="VMZ53_12715"
     CDS             complement(3301..3648)
                     /locus_tag="VMZ53_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUS29370.1"
                     /translation="MALRVRVCRLVDVADLDGDRMRGFAVTGVTWPVIVARLDGELIA
                     FPGVCPHDDVSLVSYGVIEPSEDGSGGRDLRCRVHGYRFDLATGRCEHSPGLHLRRYR
                     VTIAGDELLVDLL"
     gene            complement(3862..5259)
                     /locus_tag="VMZ53_12720"
     CDS             complement(3862..5259)
                     /locus_tag="VMZ53_12720"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HUS29371.1"
                     /translation="MKWAIVIVLVAGCYDSGTVACGDLLCPHGSVCAPEEDRCVTPNQ
                     ITACAGFAEGAGCSAGTIAGGVCHRQVCIEAGCGNSVVEAGELCDDGNRFSLDGCSSD
                     CRSNERCGNGVVDIALGEQCDCGDATVAAGCERANSENAGAECRTNCKTGRCGDGVVD
                     PGEWCDDGNTAPGDGCRADCTGRFTLMPSGTLHKLNDVWGRSPDDVWAVGEQRILHFD
                     GVLWHLVEPPALNSIYQQVCGTGPTTLYVKAYGSSPIIYRLEGSTWSTLAMPTTPSPH
                     VPGSIACAGSDLIIGNVGTSPVFVDVWNGTSFSSLPTTTTQIAAMSARSRNDIYALLL
                     SSGDTMAVRYFNGAAWIDAGYSVTSLYGKDILALSDGSAFTYSPVYRFTDAWRVQPLA
                     DDISASTVAGVPGDVILAGATPKIDFAAAAMRFDGTAWSLLPMPDLPGVRINGAWVAS
                     PGHAFLVGDSGTILY"
     gene            complement(5273..5587)
                     /locus_tag="VMZ53_12725"
     CDS             complement(5273..5587)
                     /locus_tag="VMZ53_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29372.1"
                     /translation="MRAAGVMLLLIACKPHHAMPPPAGPDQSVHDAMVLVCDAPVRAE
                     GEKAATRADAIAGHLTDGVGNAKVLDIVEAWKTDGVNRTQLTKLVSEAKLSSCALRDE
                     LR"
     gene            5611..6513
                     /locus_tag="VMZ53_12730"
     CDS             5611..6513
                     /locus_tag="VMZ53_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS29373.1"
                     /translation="MSTVLFALLALFVIIAVAVAAAIVLRPRKVAPAEGVEPIAGPAL
                     ATAPLPVVLVHGLFGFDRIGVPGVKLHYFRGIVKHLESLGCHAHAVRLPAAASVPDRA
                     KILVEKIAELGHDRVDIIAHSLGGLDARYALAKLGLAARVRSLVTIGTPHHGTPLANF
                     ATEGPLGLTRKALALVGMPMHALDWLSPAALERFNAEVPNATGVSYACVVAGIREPGT
                     PVSLAIAPAHAYLRRVSGPNDGLVPIASQYWGETLAEIEADHFEQVGWRMAMRGTFDA
                     LALYAFILARLGDAPATPAQAAAS"
     gene            6688..7329
                     /locus_tag="VMZ53_12735"
     CDS             6688..7329
                     /locus_tag="VMZ53_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUS29374.1"
                     /translation="MSRFLFPEWTETLKKWVNLLVLGAPVYLVALIAYGVTPEAIREG
                     YQPEQPVPYSHALHAGELGLDCRYCHTTVERAAKAAIPPAATCMNCHSTVKKDSDVLL
                     PIRQAFAENEAVRWTRVHDLPDYVYFNHMAHVNAAIGCESCHGRIDRMVTVYQAKPLT
                     MEWCIECHKNPAPYLRQPSMVTQMGYKPGAADGVAIMKKNEVSPPTNCSTCHR"
     gene            7339..>10449
                     /locus_tag="VMZ53_12740"
     CDS             7339..>10449
                     /locus_tag="VMZ53_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUS29375.1"
                     /translation="MSAKEHDNTHDQASGDNTASHPQAPTGPLPATPQHGFWKSLREL
                     DGKAPWQLEPHIKEFEPGTEPTATPLLDPMSRRNFFNLMGASLGLAGIAGATGCRYDK
                     EEIVPLSRRPDGHVPGQVLQYATTFELGGSAHAVVATSFEGRPIKIDGNPDHPFAGGA
                     IVAGTKRHAGAHTFAQAAILHLYDPDRSQSVLNSNKGSSFDAFKASLGDIKKALTQNA
                     RVLSEATSSPTVLALRRRLEQGGVGWHEYEPLSWDNERLGMKAAFGRSVRPIARLDRA
                     ETIVTIDCDIFVEHPAAMKYNRDFARSRRKDGSLGVGKMNRLWVAESVMSNTGAMADH
                     RLGMRAELGLPFAMALDAAVSGGGAPNSEFLKETKVAQFITILAEELKVNAGRAVVIA
                     GRRQPAEVHALVAKINQAIGAPGATLDYVEDPDPERPSHLQSITALAQDIQGGRVSTL
                     IILGGNPAYDAPADLDFARLLGDLTKKPGGVVVHLGEYADETSQLATWHLPKAHFLEA
                     WGDARTWDGTVSVAQPLINPMWGGISSAELLSMLLGDELPSEQLVKATVGGMAGGANW
                     KQVVHDGFVPGSNLATAGVQFQALPAFNLTPSQQAGSRRKNGELEVVYHYSSFTYDGR
                     FSNNPWLWETPDFLTKVTWDNYALVSPETAVSLGLENNHHMKVTVDGRSITLPCYVMP
                     GQAKSSIGLVLGGGRKVAGRSVIAGGEKSAGPGYNTYVVRSSKAFDFAANASVSNTGD
                     HYVLASVQDHWNYKPGNNKLMGYKDDAGLRFDPEVERRSREDVEETTAEELEKNRTWS
                     AAHESEFWDDTAEAATEKSKARHLSLFEEHDYHGHRWAMAIDLTTCTGCNSCMVACQS
                     ENNVPVVGRTQVNNNREMHWIRIDRYFQGSPEEPHVVHQPLACQQCENAPCEQVCPVG
                     ATQHSDEGLNDMAYNRCIGTRYCANNCPYRVRRFNFLDWNKEWREARNKVRRLLFNPD
                     VTVRMRGVMEKCTFCVQRIQNGKIKAKAQIRSGQRQGAVGAPLPDGEIETACQQACPT
                     EAIVFGD"
BASE COUNT         1876 a         3544 c         3398 g         1631 t
ORIGIN      
        1 gtgccgcacg cggactgcgt gccgttgcgg aagaggacga tgcgcgtgac ttcgtagttg
       61 tcgaacgtct tcttccaggt gttctgcacg tcgtcgaaga cgtagccgac gaagttgacg
      121 aggtcgtctt cttcggcgga cgactggacg ggcccgccgc cggtgccgcc gccgctgcgc
      181 gtcgggctcg tgggttggct gccgcctccg ccgagcgcgc aaccttggcc gccgccgagg
      241 cacgtgccgc cgccggcgag gacggccatg agcacgaggc cgatgaggat gcctttccag
      301 ccgaacatcc cgagcaggcc gaggatgttg aacccgccgc cgccgccgcc gaagccgaag
      361 ccgccgcctc cgccgctgaa gccttcgccg cgacgatctt cgatgttcga gcttctgtag
      421 gatcgatccc aacgcatact cgaatcgaag catcgagtgc tgcgagcgac aagcagcgcc
      481 acgtctttct cgcgggcgaa gttttcggtg ggcggtcgcg cgcgcgtggc ggctgcgcaa
      541 cggtcggcgc cgaattgcga tcagcggtgt gtcagctcaa cgcatcgttg cgccggcgct
      601 cgtagagcca gcgcaggatc gcgtcggcct tcgctcgtgt gagcgcggcg tcttcgcggg
      661 ggtggttggc gcccggcacg agatcccact tcacttcttg gccgcagcga tcgaagtgat
      721 cgcggaggcg gactgcggcg ccgtgggcgg gattttgatc gccgacgagg aagtaggcgg
      781 ggaatgtgcg atcgatgcag tgcgcgttcc gcggcgggac gccgcctccg tggatcacga
      841 ccgccgcgaa caagtcgggc cacgcttgca ggtgcatgcc gatgtacgtc gcgccgccgg
      901 accagccgac gagatagatc cgcgtcgggt cgatcgcgcg ctttgacgtg acgtcgtgaa
      961 cgcgatcgcg cagccaccgc gggttgccgt tccaccgata ccagctgccg tcttcgcagc
     1021 cgcgatcgcg cgggcagtcg agcgcgagca gctcccagcc gcgcgcttct accgccgttt
     1081 gccagcggcg aaggctcgcc cgcgagtctt cgtgatctcc gtgcagaacg acgacgagtg
     1141 gaactgcttc cgtttctgcg gacgcggcat ggaggcgaaa cgtcgactga ccgcgcagcg
     1201 tgacacctgc gcccgccgca actccagcaa ggatgaggac gagcaacgcg taacgcatac
     1261 cgtcggagat tgcatctcgc ttgccgtcac agacatgtgc gacggacccg cacagcgcac
     1321 ggccgtcgtc gtgaatccgt cacgtgctgc tctacggaaa cgcggcacac tttcagcacg
     1381 tgcgcgcgca gttcgtcctc tcgatagcag ccgtagcggg cagcatctcc gcgtgttcga
     1441 cgccgacgtc cggaaatcgg acaaccacgt cgccggtgac gaccgcacgg gcaagctcgc
     1501 aggcgctcgt cccgaagctg acagcggaag gcgaatgcat ccacgtgcag ctgttcgagg
     1561 acggtcaaga caagggccca atctgtatcg ccgacgcgca ggcgcgcggg ctgaccgtcg
     1621 tcgatctgac ggatcagtgg acgccgacgt tgttcgatcc taccgacggt caagcgccga
     1681 cgtttcgcgc gcgctatctc gagctcgctg cggaaaagag cgaaggcgat agtctcgaag
     1741 agctgaccga gctgtacggc gtcgtgcccg cgctgtcgat cgtccacaag cggttggcgg
     1801 atgaccaacg ccacgcgtgt cacgccgcga tcgatccctc gccgattctc gcgctcgaca
     1861 cgacgctctc gcaggggaac aaggacaccg tgcaggccgg catcgtcggt cgcgactggc
     1921 tcgagaaaga gctcgagaag gaacgcgcgc gtcagaagct gcccgacatc gctgcgctcg
     1981 aagccgatcc gaagtggcac gaccgcttca tccgctacaa gaagctcgat cagacgcaca
     2041 ccgcattgct cgccgcacaa cagcaactgc attgcgaagg ctggatgcag gaccaagacg
     2101 tcgacggcac gtttacgtgg cgcatgggta tggccgtcga gatgttccag cggcgcaact
     2161 tcctcatgcc gacgcagcgc ctcgatccgg agacgcgcga agcgctgcag ctcgacagtc
     2221 gcgagctcga cttccgtctt gcacttcgca tcctgcgcga gcgcgtgacg gcggccgcgg
     2281 ggctcatcga ggacggcacc gccggtgagg gtccgcagcc cgtgctcggc cgccagctcg
     2341 atccaccggc gatgcgtgcc gcgcgcgggc acaagccgat ggcgaatgca gcgcccgatc
     2401 tcgtcgacct cgcgacggag gctgctgcga agcagctcgg gtggacgacg ccgaacgacg
     2461 tgcgcgcgtt cctagacagg atgggcggct ccggcgtgcg cgtcgcgatc gagctaccgc
     2521 cgctgcccgc ttatcacagc acgcacatgg acatcaccgc cgagatcgat cgcggcgaca
     2581 tctactacga ggatcaaccg ccaccgttca agcgcatcct gccgcaccgg ccgacgctcg
     2641 tgctctacgc gatggacggc gagaccaagc gtccactcgt gcgctggccg acgacggtcg
     2701 gtgggtgggc cgatcagcga ttgaagagcg gccgcatcgt ccagaagtgg aaggagtccg
     2761 acgtcgggcc gcgcgtctgg aaggatctca ccgcggcgcc gacgtggatc ccgccgaaga
     2821 cgacgcccga taaagatctc gtgaagccca cgtatcccgg ctgggcgctg aagtccgaga
     2881 tcatggggcc gggaccgcgc gcggcgtacg gcatggtgct gctcgagcac gtgctgccgg
     2941 tgaagctcaa ggacggcacc gagcggttcg acgacaacgg catcggcacg catggctcgt
     3001 cgagcgtcac gtcgatcgtc ggcggcaaca gccacggctg ccatcgcctc tacaaccagc
     3061 tcgcggtgcg cctcggcgac ttcctgctcc accatcgcaa ccacgtcgtg aagggcgaga
     3121 cgcccgtcgg cttccgtcgc atcgtctggc acagcggcga agcgttccgc gccgagatcg
     3181 aatcgcgcgg ctttcagtac gagctcacgc cgccggtgcc ggtcaacgtg ctcaagggca
     3241 acatcctcag caagcgcaag acgccgccga agaacagcgc gcccgcgcgg ccgtaggcgc
     3301 ctacagaaga tcgacgagca gctcgtcgcc ggcgatcgtc acgcgatagc ggcgcaggtg
     3361 cagacccggc gagtgctcgc agcgtcccgt cgcgagatcg aagcgatagc cgtgcacgcg
     3421 gcagcgcagg tcgcgaccac cgcttccgtc ttcgctcggc tcgatcacgc cgtagctaac
     3481 gagcgagacg tcgtcgtgcg ggcatacgcc gggaaacgcg atgagctcgc catcgagccg
     3541 cgcgacgatc acgggccacg tcacacccgt caccgcgaag ccgcgcatgc gatcaccgtc
     3601 gaggtccgcg acatcgacga gccggcagac gcgaacacgc agcgccacga ataatgagcg
     3661 tacccgatcg ccgacgacgc gccgacccgc agacgagcac ggcgctacga aaccggtcgt
     3721 accgatcgcc gacgatgcgc cgacccgcag acgagcgcag cggtacgatc gaggatcgta
     3781 cccgatcgcc gacgatgcgc cgacccgcaa gcgcgcacag cgcgacgaga gccgacgctg
     3841 ctgttcgccg acgacgcgcc gttagtagag gatcgtgccc gaatcgccga cgagaaatgc
     3901 gtggccaggg ctcgcaaccc acgcaccatt gatccgcacg ccgggcaagt ccggcatcgg
     3961 caagagggac cacgcggttc cgtcgaatcg catcgccgct gccgcgaaat cgattttggg
     4021 agtcgccccg gcgaggatca cgtcgccggg aacgccggcc accgtcgatg ccgagatgtc
     4081 gtcggccaac ggttgtaccc gccacgcgtc cgtgaagcga tagacgggcg aatacgtgaa
     4141 cgcactgcca tcagagagcg caagaatgtc tttgccgtac agcgaggtga ccgagtagcc
     4201 agcgtcgatc catgcggcgc cgttgaagta gcgcacggcc atcgtgtcgc cactcgacaa
     4261 caacagcgcg tagatatcgt ttcgactgcg cgccgacatg gccgcgatct gagtcgtggt
     4321 tgtcggcagt gacgaaaagc tcgtgccgtt ccagacatcg acgaaaacgg gcgacgtgcc
     4381 gacattgccg atgatcagat cagagcctgc gcacgcgatg cttccaggaa cgtgtggcga
     4441 tggcgtcgtc ggcatcgcga gcgtcgacca cgtggaccct tcgaggcggt agatgatcgg
     4501 cgaggaaccg tacgctttca cgtacagcgt cgtcggcccc gttccgcaca cctgttgata
     4561 gatcgagttc agggcgggtg gctcgacgag gtgccagagc acgccgtcga aatggagaat
     4621 gcgctgctcg ccgacggccc acacatcgtc aggtgagcgg ccccacacat cattgagctt
     4681 gtgcagcgtt cccgacggca tcagcgtgaa gcgccccgtg cagtccgcgc gacagccatc
     4741 gcccggagcg gtgttgccgt catcgcacca ctcgcccgga tcgacgacgc catcgccgca
     4801 ccgaccggtc ttgcagttcg tgcggcactc ggcgcctgcg ttctcgctat tcgcgcgttc
     4861 gcatcccgca gccaccgtcg cgtcgccgca atcgcattgc tcgccgagcg cgatgtcgac
     4921 gacaccattg ccgcatcgct cgttggagcg acagtccgag ctgcagccgt cgagcgagaa
     4981 tcggttgccg tcgtcgcaca gctcgcccgc ttccacgacg ctgttgccgc aacctgcttc
     5041 gatacagacc tggcgatggc agacgccgcc ggcgatcgtg cctgcgctgc aaccggcgcc
     5101 ttcggcgaag cctgcacacg ccgtgatctg gttcggcgtg acgcagcgat cttcctcggg
     5161 cgcgcagacg ctgccgtgag ggcagagcag gtcgccacac gcgacagtgc cgctgtcata
     5221 gcagcccgca acgagcacga tcacgatcgc ccacttcacg aacgcgatcg tatcaccgca
     5281 gctcgtcgcg gagcgcgcag ctcgagagct tcgcttcgct gacgagcttc gtcagctgcg
     5341 tgcgattcac gccgtcggtc ttccacgctt cgacgatgtc gagcaccttc gcgtttccga
     5401 cgccgtcggt cagatgtccg gcgatcgcat ccgcgcgtgt cgctgccttc tcgccctcgg
     5461 cgcgcacggg tgcatcgcac acgagcacca tcgcatcgtg cacggattga tcgggcccgg
     5521 ccggcggcgg catcgcgtga tgcggcttgc aagcgatcag gaggagcatc accccggcag
     5581 cgcgcatgcc ctgatcctat actgctcgca gtgagcaccg tcctcttcgc gctgctcgcg
     5641 ctcttcgtca ttattgcggt cgcggtcgcc gccgcgatcg ttctgcgccc gcgcaaggtc
     5701 gcccccgccg agggcgtcga gccgattgcc ggcccggcgc tcgcgaccgc gccgctgccc
     5761 gttgtcctcg tgcacggcct gttcggcttc gatcgaatcg gcgtgcccgg cgtcaagctc
     5821 cactacttcc ggggtatcgt gaaacacctg gaatcgctcg ggtgccacgc gcacgctgtc
     5881 cggctcccgg ccgccgcgag cgtgcccgat cgcgcgaaga tcctcgtcga gaagatcgcc
     5941 gagctcggcc acgaccgcgt cgacatcatc gctcacagcc tcggcggcct cgacgcgcgg
     6001 tatgcgctcg cgaagctcgg gctggcagcg cgtgtccggt cgctggtcac gatcgggacg
     6061 ccgcatcacg gaacgccgct cgcgaatttc gcgaccgaag gcccgctcgg tctcacgcgc
     6121 aaggcgctcg cgctggtcgg catgccgatg cacgcgctcg attggctctc gccggccgcg
     6181 ctcgagcggt tcaacgccga ggtcccgaac gctaccgggg taagttatgc ctgcgtcgtt
     6241 gctggcattc gcgagcccgg gacgcccgtg tcgctcgcga tcgcgccggc gcatgcgtat
     6301 ctgcggcggg tgagcggccc caatgacggc ctcgtgccga tcgcttctca atattggggc
     6361 gagacgctcg cggagatcga agcggatcac ttcgagcagg tcggctggcg catggcgatg
     6421 cgaggcacgt tcgatgcgct cgcgctgtac gccttcatcc ttgctcggtt aggggacgct
     6481 cccgcgaccc ccgcgcaggc tgcggcgtcc tgacgcaggc accgtgcggt cttttgtcgc
     6541 gttgggaatc aacgacgttt tgccgcacgc gaatgttcga cgaggcatgc gatcacacgc
     6601 atcggacgtc agggcgatct ccaagatcgc cgcctcgcaa ccttgcgaag tacttacgag
     6661 tcgtgatgaa agccgcgtag ggaaaccatg tcgagattcc tatttccgga atggaccgag
     6721 accctcaaga agtgggtcaa cctgttggtc ctcggcgcgc cggtgtatct ggtcgcgctc
     6781 atcgcttatg gcgtcacgcc cgaagcgatc agagagggct atcagcctga acaacccgtg
     6841 ccgtacagcc atgcgctcca cgcaggcgag ctcggcctcg attgtcggta ctgccatacg
     6901 acagtcgaac gcgcagcgaa ggcggccatc ccgcccgcgg cgacgtgcat gaactgtcac
     6961 tcgacggtga agaaggacag cgatgtcctc ctgccgatcc ggcaggcctt cgccgagaac
     7021 gaggcagttc gctggacgcg cgtccacgac cttcccgact acgtctactt caatcacatg
     7081 gcgcacgtga acgcagcgat cggctgcgag tcgtgccatg gccgcatcga tcggatggtc
     7141 acggtctacc aggccaagcc gctgacgatg gagtggtgca tcgagtgcca caagaaccca
     7201 gcgccgtatc tgcgccagcc ctcgatggtg acgcagatgg gctacaagcc cggcgccgcc
     7261 gatggtgttg cgatcatgaa gaagaacgaa gtcagcccgc ccaccaactg ctcgacctgt
     7321 caccgctagg agccgagcat gtctgccaaa gaacacgaca acactcacga tcaggcgagc
     7381 ggcgacaaca ccgcttcgca tccccaggct cccacgggtc cgctgccggc aacgccgcag
     7441 cacggcttct ggaagtcgtt gcgcgagctc gacggcaagg cgccgtggca gctcgagccg
     7501 cacatcaaag agttcgagcc cggcaccgag ccaacggcga cgccgctgct cgatccgatg
     7561 tcgcgccgta acttcttcaa cctgatgggc gcgagccttg gcctcgccgg aatcgccggc
     7621 gccacgggtt gccgctacga caaagaagag atcgttccgc tctcgcgtcg ccccgacggc
     7681 cacgtgccgg gtcaggtgct gcagtacgcg acgacgttcg agctcggcgg ctctgcacac
     7741 gcagtcgtcg cgacgtcgtt cgagggccgc ccgatcaaga tcgacggcaa ccccgatcat
     7801 ccgttcgcgg gtggcgcgat cgtcgccggc acgaagaggc acgcaggcgc gcacacgttc
     7861 gcgcaagcgg cgatcctcca cctctacgat ccggaccgct cgcagagcgt cctcaacagc
     7921 aacaagggca gctcgttcga cgcattcaag gcgtcgctcg gcgatatcaa gaaggcgctg
     7981 acgcagaacg cgcgcgtgct cagcgaagcg acgtcgtctc cgacggtgct cgcgctccgt
     8041 cgccgcctcg agcagggcgg tgtgggctgg cacgagtacg agccgctgtc gtgggacaac
     8101 gaacgcctcg gcatgaaggc agcgtttggt cgcagcgtgc ggccgatcgc gcgcctcgat
     8161 cgcgccgaga cgatcgtcac gatcgattgc gacatcttcg tcgagcaccc cgcggcgatg
     8221 aagtacaacc gcgacttcgc gcgcagccgc cgcaaggacg gctcgctcgg tgtcggcaag
     8281 atgaaccgcc tctgggtcgc agagagcgtg atgagcaaca ccggcgcgat ggcggatcac
     8341 cgcctcggta tgcgcgccga gctcggcctt ccgttcgcga tggcactcga cgctgcggtc
     8401 agcggcggcg gggcaccgaa cagcgagttc ctcaaggaaa cgaaggtcgc gcagttcatc
     8461 acgatcctcg ccgaggagct caaggtcaac gcaggccgcg cggtcgtcat cgccggtcgt
     8521 cgtcagcctg ccgaagtcca cgcgctcgtc gcgaagatca accaagcgat cggcgcgccc
     8581 ggtgccacgc tcgactacgt cgaggatccg gatcccgagc gtccgagcca tctgcagtcg
     8641 atcaccgcgc tcgcgcagga tatccaaggc ggccgcgtct cgacgctgat catcctcggc
     8701 ggcaaccccg cctacgatgc gccggcggat ctcgatttcg cgcgcctcct cggcgatctc
     8761 acgaagaagc ccggcggcgt cgtcgttcac ctcggcgagt acgccgacga gacctcgcag
     8821 ctggcgacct ggcacctgcc gaaggcgcac ttcctcgagg cgtggggcga cgcgcgcacg
     8881 tgggacggca ccgtgtccgt cgcgcagccg ctcatcaacc cgatgtgggg cggcatctca
     8941 tcggccgagc tgctgtcgat gctcctcggg gacgagcttc cgagcgagca actcgtgaag
     9001 gcgacggtcg gcggcatggc cggcggcgcg aactggaagc aggtcgtgca cgacggcttc
     9061 gtcccgggct cgaacctcgc gaccgcgggc gttcagttcc aggcgctgcc cgcgttcaat
     9121 ctgacgccga gccagcaagc cggcagccgc cgcaagaacg gcgagctcga agtcgtctat
     9181 cactactcga gcttcacgta cgacggccgg ttctcgaaca acccgtggct gtgggagacg
     9241 cctgacttcc tcacgaaggt cacgtgggac aactacgcgc tcgtctctcc cgagacggcc
     9301 gtgtcgctcg gtctcgagaa caaccatcac atgaaggtca cggtcgacgg caggtcgatc
     9361 acgctgccct gctacgtgat gcccggccag gcgaagtcgt cgatcggtct cgtcctcggc
     9421 ggtggccgca aggtcgccgg tcgcagcgtg atcgccggcg gcgagaagtc ggcgggcccc
     9481 ggttacaaca cgtacgtcgt tcgctcctcg aaggcctttg acttcgcggc gaacgcctcg
     9541 gtgtcgaaca ccggcgatca ctacgtgctc gcgagcgtcc aggatcactg gaactacaag
     9601 cccggcaaca acaagctgat gggctacaag gacgacgcgg gcctccgctt cgatcccgag
     9661 gtcgagcgtc gctcgcgcga ggacgtcgaa gagacgaccg cggaagagct cgagaagaac
     9721 cgcacctgga gcgccgcgca cgagagcgag ttctgggacg acaccgcgga agccgcgacc
     9781 gagaagtcga aggcgcgcca cctctcgctg ttcgaagagc acgactatca cggccaccgt
     9841 tgggcgatgg cgatcgacct cacgacgtgc acgggctgca actcctgcat ggtcgcgtgt
     9901 cagtcggaga acaacgtgcc ggtcgtcggc cgcacgcagg tgaacaacaa ccgcgagatg
     9961 cactggatcc gcatcgaccg ctacttccag ggcagcccgg aagagccgca tgtcgtgcat
    10021 cagccacttg cgtgtcagca gtgcgagaac gcgccgtgcg agcaggtgtg cccggtcggt
    10081 gcgacgcagc acagcgatga aggcctgaac gacatggcct acaaccgctg catcggcacg
    10141 cgctactgcg cgaacaactg cccgtaccgc gtccgtcgct tcaacttcct cgattggaac
    10201 aaggagtggc gcgaggcgcg taacaaagtc cgtcgcctcc tgttcaaccc ggacgtcacc
    10261 gtgcgtatgc gcggcgtgat ggagaagtgc acgttctgcg tgcagcgcat ccagaacggc
    10321 aagatcaagg cgaaggcgca gatccgcagc ggtcagcggc agggcgcagt cggcgctccg
    10381 ctccccgatg gcgagatcga gacggcgtgc cagcaggcgt gcccgaccga ggcgatcgtg
    10441 ttcggcgat
//
LOCUS       DATLBZ010000239         3300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6090601, whole genome shotgun sequence.
ACCESSION   DATLBZ010000239 DATLBZ010000000
VERSION     DATLBZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6090601"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VMZ53_12745"
     CDS             complement(<1..635)
                     /locus_tag="VMZ53_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29376.1"
                     /translation="MGRVATNALLLVLAGCNQFFDLDTTQLTDASHPFFDAAPDAPPM
                     CADPSMTPRFDRELHQAVYIDEKCESYQTSAVGLALAACDLSKLNPAIREAPVGPWKE
                     MTLAKGNIQQANVVYNLALAPEGNEVFLARYDAVLGAGIWRYARDANGAWNLQEKLPV
                     TFTAAQRISTVTRGPKRHMFVIETNQQLHEWATDDNGTWSSVRTSTATNLGV"
     gene            complement(636..1097)
                     /locus_tag="VMZ53_12750"
     CDS             complement(636..1097)
                     /locus_tag="VMZ53_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29377.1"
                     /translation="MLLVALIVVGSIVALVVVMALIGLALPRDHVATRSAVLGAAPAD
                     VWREMVAVSQEPRFQQGITFEIDEDRPPGSGQAGRRVTRIADDKLPFGGRWIYEVAAE
                     GSGSRITITEEGFVKNPVFRLLSATVFKQTATMEKLIRALAAKLGVDVAWR"
     gene            complement(1253..1984)
                     /locus_tag="VMZ53_12755"
     CDS             complement(1253..1984)
                     /locus_tag="VMZ53_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29378.1"
                     /translation="MIIIYGQRLYGAVDEQGGQYAVTKFAHIYYMPLFPVGSVWLTAP
                     DRGIPTPLNGKSVLAAYARTWGLVAGGALIVSGLSTSIIVSILGLVVLAASIATWKLW
                     GKRATHDAQLRGNLNALAFGTYCDPQLFTPPMREHYKLELQRRQQVAEHARPPEDVAR
                     FGTRDLGELVRSYGILSLHGSAQAREQLSQLLKLDAPSAQLTDGGIYREKHGDDATET
                     LQLPADRVQMLAAIDDIARQRMLRA"
     gene            2069..>3300
                     /locus_tag="VMZ53_12760"
     CDS             2069..>3300
                     /locus_tag="VMZ53_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29379.1"
                     /translation="MAVSIASPTLAAYGAGGGGGGGGGGGGPSSGGGGPSGGGGGGGG
                     GGGGGGGGGGGGGGGGGGTGQGGARGQGGGGGAAGKVGGAVVVHKSVGGPPPPEKIEL
                     KSYLGVFRYIRRAIELVWSTNRGLTIGLALGSLLAGLLPTGIAYVGKRLINSIDIARN
                     LPGHDHSSAMMWVGAELALVVGLALVQRTLGIFRSLLRQQLGQRINVLVLEKALTLEL
                     TQFEDSELYDKMTRARREASSRPLSLVSQAFELGQSMVTLIGLGGLLAAFSPIALCVL
                     IAAAIPPFVAELKFSGDAFRLSRWRTPETREQMYVEMVIAQDAYAKEVKLFGLGPRFL
                     RRYREIYEKLYAGDRKITISRGIWALILGTIGQAAFYGMYLWIAFATIDNTISFGDMT
                     MYVLVFKQAQSALSSALG"
BASE COUNT          529 a         1051 c         1133 g          587 t
ORIGIN      
        1 acgccgagat tcgtcgcggt ggacgtgcgc acggagctcc acgtgccgtt gtcgtcggtc
       61 gcccactcgt gcagctgttg attcgtctcg atgacgaaca tgtggcgctt cggcccgcgc
      121 gtcaccgtcg agatccgctg cgccgccgtg aacgtgacgg gcagcttctc ctggagattc
      181 cacgcaccgt tcgcatcgcg cgcgtaccgc cagatgccag cgccgaggac cgcgtcgtag
      241 cgcgcgagaa acacctcgtt gccttccggc gcgagcgcga ggttgtagac gacgttcgcc
      301 tgctggatgt tgcccttcgc gagcgtcatc tccttccacg gaccgaccgg cgcctctcga
      361 atcgcgggat tgagcttgct caggtcgcac gcggcgagtg cgagaccgac cgcgctcgtc
      421 tgatacgact cgcacttctc gtcgatgtag acagcttgat gcagctcgcg atcgaaccgc
      481 ggcgtcatgc tcggatcggc gcacatcggc ggcgcgtcag gggccgcgtc gaagaaggga
      541 tgactcgcgt ctgtcagctg cgtggtgtcg aggtcgaaga actggttgca gccggcgagc
      601 acgagcagca gcgcattcgt cgcgacccgt cccattcatc tccatgctac gtcgactccg
      661 agcttcgcgg cgagtgcgcg gatgagcttc tccatcgtcg cggtttgctt gaacaccgtc
      721 gccgaaagaa gccggaacac cgggtttttc acgaagcctt cctcggtaat cgtgatgcgc
      781 gagccgcttc cctcggcggc gacctcgtag atccagcgcc cgccaaacgg cagcttgtcg
      841 tcggcgatcc gggtgacgcg cctgcccgct tggccgctgc cgggagggcg gtcctcgtcg
      901 atttcgaacg tgatgccctg ctgaaagcga ggctcctgtg agaccgcgac catctcgcgc
      961 cagacatcgg ccggtgcggc gccaaggact gcgctgcgtg tcgccacgtg atcgcgcggc
     1021 agcgccaacc cgatgagcgc catcacgacg acgagcgcga cgatcgaacc aacgacgatg
     1081 agcgcaacga gcagcatgag actcggttac cgcgcgcgtc gatacttcgt caaaacgaac
     1141 aatcaggcgc ggcggcgggt cgtgtgctcg acgtgacacg cgagatagag ctgcgggtat
     1201 gcgcgctgga tgcgccggac gtgatcgctg gctcgaaccc gcgacatcga ggtcaggcgc
     1261 gcagcatccg ctggcgagcg atgtcgtcga tcgccgcgag catctgcacg cggtcggcgg
     1321 ggagctgcag cgtttcggtc gcgtcgtcgc cgtgcttctc gcggtagatg ccaccgtcgg
     1381 tgagctgtgc cgacggcgcg tcgagcttca ggagctgcga gagctgctca cgcgcttggg
     1441 cgctgccgtg caaactgagg atgccgtacg agcggacgag ctcgccgagg tcacgcgtgc
     1501 cgaagcgtgc gacgtcctcc ggtgggcgcg catgctcggc gacttgctgg cggcgttgca
     1561 gctcgagctt gtagtgctcg cgcatcggtg gcgtgaacag ctgcgggtcg cagtacgtgc
     1621 caaatgcgag cgcgttgaga ttgccgcgca gctgcgcatc gtgcgtcgcg cgcttgcccc
     1681 atagcttcca cgtcgcgatc gatgccgcga gcacgacgag gccgagaatc gacacgatga
     1741 tgctcgtcga caagccggac acgatcaggg cgccgccggc gacgaggccc caggtgcgcg
     1801 cgtacgcggc gaggacgctc ttgccgttga gcggtgtcgg gatgccgcgg tcgggtgcgg
     1861 tgagccagac cgagccgacg ggaaacagcg gcatgtaata gatgtgcgcg aatttcgtga
     1921 cggcgtactg cccgccctgc tcgtccaccg cgccgtacag ccgctgcccg tagatgatga
     1981 tcacgacgcc ctcaaaggtg cacgagcctg cccgaaaagt cagcgaagaa gggccggctg
     2041 atcacgcgag tcgtcataca gtggaggcat ggctgtctcg atcgcgtcac caactctcgc
     2101 tgcatacggc gcgggtggcg gtggaggtgg aggcggcggc ggtggtggcc caagcagtgg
     2161 cggtggtggc ccgagtggcg gtggtggtgg tggcggcggt ggcggcggtg gtggtggcgg
     2221 tggcggtggc ggtggcggcg gtggtggcgg cggcaccggt cagggcggcg cacgtggcca
     2281 aggcggtggc ggtggcgctg cgggcaaagt gggcggcgcc gtcgtcgtcc acaagtctgt
     2341 cggtggccca ccacctcccg agaagatcga gctcaagagc tacctcggcg tgttccgcta
     2401 tatccggcgc gcgatcgagc tcgtgtggtc gacgaatcgt ggcctcacga tcggcctcgc
     2461 actcggcagt ttgctcgccg gtctgttgcc gaccggtatc gcgtatgtcg gcaagcggct
     2521 gatcaactcg atcgacatcg cgcgcaacct cccgggccac gatcactcgt cggcgatgat
     2581 gtgggtcggc gccgagctcg ctctcgtcgt cggcctcgcg ctcgtgcagc gcacgctcgg
     2641 catcttccgc tcgctgcttc gccaacagct cggccagcgc atcaacgtgc tcgtcctcga
     2701 gaaggcgctg acgctcgagc tcacgcaatt cgaagacagc gagctgtacg acaagatgac
     2761 gcgtgcacgt cgcgaagcgt cgtcgcggcc gctgtcgctt gtctcgcagg cattcgagct
     2821 cggccagagc atggtcacgc tgatcggcct cggcggtctg ctcgcagcgt tctcgccgat
     2881 cgcactttgc gtattgatcg ctgctgcgat tccgccgttt gtcgccgagc tcaagttctc
     2941 gggcgatgca ttccggctct cgcggtggcg cacgcccgag acgcgcgagc agatgtacgt
     3001 cgagatggtg atcgcgcagg acgcatacgc aaaggaggtc aagctcttcg gcctcggccc
     3061 gcgcttcctt cgccgctatc gcgagatcta cgaaaagctc tacgcaggcg atcgcaagat
     3121 cacgatcagc cgcggcatct gggcactcat cctcggcacg atcggtcaag cggcgttcta
     3181 cggcatgtac ctgtggatcg cgtttgcgac gatcgacaac accatctcgt tcggcgacat
     3241 gacgatgtac gtcctcgtct tcaagcaagc gcagtccgcg ctatcgagcg cgctcggcga
//
LOCUS       DATLBZ010000240         8304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9196707, whole genome shotgun sequence.
ACCESSION   DATLBZ010000240 DATLBZ010000000
VERSION     DATLBZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8304
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9196707"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VMZ53_12765"
     CDS             complement(<1..528)
                     /locus_tag="VMZ53_12765"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HUS29380.1"
                     /translation="MAETIRAPRGSQLTCKGWQQEAALRMLMNNLDPDVAERPQDLVV
                     YGGTGKAARSWEDYHVIVRELRKLENDQTLLVQSGRAVGVFRTHEEAPRVLIANSNLV
                     GKWANWEHFRELEGRGLMMYGQMTAGSWIYIGSQGIVQGTFETFAAAGKTHFGSDLAG
                     RVVLSGGLGGMGGAQP"
     gene            complement(960..2000)
                     /locus_tag="VMZ53_12770"
     CDS             complement(960..2000)
                     /locus_tag="VMZ53_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUS29381.1"
                     /translation="MSATPNPIDVVERAYDLDAPMKPWLEALLRSIAPLIDGDRGFMA
                     YEFDMTTLPARWLESGVTIDFDVDLYDVLQAMMGSYDRADVNRFQVAPTTLTTLGQTS
                     KQIGVADPRKHPEFARLIAKLGAVDMAAYRTVEPRGKGLAFCAPQRRDRSFDPRVRRL
                     WAKVAAHVAAGHRLRDRLASATGTGAKTTAARTSEAFDAVLTPSGKLEHAEGETKHGS
                     TREALRTAVQRQERARGRARREDPEAATTMWTALVAGRWSLVDHFESGGRRYIVARRN
                     EHGGPDPRALGARERDVAQLAALGKSNKLIAYELGIAESTVASHLSSAMRKLGVKTRV
                     ELLRMLMQLAQA"
     gene            complement(1997..2299)
                     /locus_tag="VMZ53_12775"
     CDS             complement(1997..2299)
                     /locus_tag="VMZ53_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HUS29382.1"
                     /translation="MVRPRGVEQFEVEIGGERLVVTSLPADSAADTAETLAALTAAER
                     AVACDAAAGLSNAAIAKKRKRAVRTIANQLASIYRKLGVVSRAELALLVLGAADLL"
     gene            2490..3230
                     /locus_tag="VMZ53_12780"
     CDS             2490..3230
                     /locus_tag="VMZ53_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HUS29383.1"
                     /translation="MSIETIANRLARANDFVQVAIEVCAMTRELFEVVECGVGLHAPG
                     GRPTLAVDNLRLPSDEPRLQWLAGAWERDPLHRELIAMTAPVEDGAHFIEFARRSGYT
                     GPTLHMLLLPLIYPRGLLGHIRCGKPAPFSAEARRDLQTLATAASVRLTELHVQPGDD
                     PRLARLTARQRDVARLAGKGMSNAEIGRTLDLSHNTIKKHLKDVFETLGVTSRAQLAV
                     IMCDIGPVHDYPFGITRVDDITIMRGAL"
     gene            3261..4067
                     /locus_tag="VMZ53_12785"
     CDS             3261..4067
                     /locus_tag="VMZ53_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29384.1"
                     /translation="MLVVAGTLVYAALQNDVLSELIPPMDDLGFTHDNVLAIGVARGD
                     YVIGGKLFDRWITSRTDRRRWDQLDLLATYEQRLWPAGDEPSQWQLTAQARLGPTFGG
                     NFGGQYLQNGWHYISKTGPTVDEGLANDYRGDDTIGVLAGAHLHAERWLRSEPFREGV
                     APSSPLRAFVYSNVDGQGTFGAGVSSIEGALGAWIGVHRIGAHVEAAVTRYHVADANL
                     ALPGGYGVGWQFEWRAGIDVTWSRYRLAYEYRANEGGSGEPIGVVAFSLH"
     gene            complement(4192..6786)
                     /locus_tag="VMZ53_12790"
     CDS             complement(4192..6786)
                     /locus_tag="VMZ53_12790"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HUS29385.1"
                     /translation="MTSLIPWLATAAEGTAEHGGRPHSEAQLTLPDLHQVKLMGMSGW
                     NILAIGLAVCAIGLLFGLIKYQKLKNLPAHKAMIEISELIYSTCKTYLRTQAKFIFQL
                     WVLIAAVIVVYFAVLETPRVPIPKVACIAAFSVIGILGSSLVAFFGIRVNTFANSRAA
                     FSSLRGKPYGTYAIPLEAGMSIGTVLISVELLLMLIIMLFLPGDYAGPSFIGFAVGES
                     LGAAALRIAGGIFTKIADIGSDLMKIVFNIKEDDARNPGVIADCTGDNAGDSVGPTAD
                     GFETYGVTGVALITFILVAFPNIPGMPALLGNDVKDMGEWIQTVLLVWLFAMRIIMIL
                     ASVGSYLLNEAVTKARFANAKTMNFEHPLTQLVWLTSLVSVGLTFGVSYLLIGQGDST
                     LPHGLWWKLSAIVSCGTLAGAIIPELVKVFTSVSSGHVRETVTASKEGGPSLNILAGL
                     TAGNFSAYWMGLVIAGLMGIAYAISSAIGIQDLLLRSNDQAHMAISPFAAQAAGTVFA
                     FGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSLIESRPGIEDEIEKDFKFRPDFE
                     KAKAFLEENDGAGNTFKATAKPVLIGTAVVGAATMIFALIMLVTNGLQPEAVGKLSMI
                     HPPFLLGLVVGGAIIYWFTGASTQAVSTGAYRAVEFIKKNIKLDTSAQAKASTEDSNK
                     VVAICTEYAQKGMVNIFMVVFFSTLAFANLDPYFFIGYLISMALFGLYQAIFMANAGG
                     AWDNAKKVVETELRAKGTDLHAATVVGDTVGDPFKDTSSVALNPVIKFTTLFGVLAVG
                     LALQLHERGLDNVKYTVAGIAFLISLFFAHRSFYGMRIATTPMTAESVRADAKAAADA
                     KAESQKEA"
     gene            6950..>8304
                     /locus_tag="VMZ53_12795"
     CDS             6950..>8304
                     /locus_tag="VMZ53_12795"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HUS29386.1"
                     /translation="MRRPEILAPAGDRDALAAALAAGADAVYFGLDDGFNARARAENF
                     SSTNLASIVAQIHRAGVRAYVTLNTLVFEPELPIVEELIRRVAAAGVDAIIVQDPAVA
                     LLARAISPHASSPPHASAPNASRVASRALEIHASTQMTASSPLATRLLAPALDLSRVV
                     VPRELSVDEIRAYREGTNVELEVFVHGALCVAWSGQCLSSEAWGGRSANRGQCAQACR
                     LPYSLVVDGAARDLGEVEYLLSPKDLVGLDAIADLAKIGVASLKIEGRLKGPGYVATA
                     VAQYRHAAASSVGERAPTPPAGVDIPLDRESLHVAYSRGVSRGFLTGADHQTLVEGRF
                     PRHRGLPLGRVIAIEGDEVELVHDPAQRPVTGGLALELESRTSDTAPNGRTSDTDDRS
                     TDDRSTDDRYSDGRHPDGHVRLSDVRAGMGIVFDSGRPEDEEQGGPIFAVAETPRGYR
                     VR"
BASE COUNT         1403 a         2791 c         2681 g         1429 t
ORIGIN      
        1 gggctgcgcg ccacccatgc cgccgaggcc gccggacagc acgacgcgtc ccgccagatc
       61 gctgccgaag tgggtcttgc ccgccgccgc aaacgtctcg aacgttccct gcacgatgcc
      121 ttgcgagccg atgtagatcc acgagcccgc ggtcatctgg ccgtacatca tcaagccgcg
      181 gccctcgagc tcgcggaagt gctcccagtt cgcccacttc ccgacgaggt tcgagttcgc
      241 gatgagcacg cgcggcgctt cttcatgcgt ccgaaacacg ccgacggccc tgcccgactg
      301 cacgagcagc gtctgatcat tctcgagctt gcgcagctcg cggacgatca cgtggtagtc
      361 ctcccagctt cgcgctgcct tgcccgtccc gccgtagacg accaagtcct gcggccgctc
      421 ggccacatcc ggatcgaggt tgttcatcag catgcgaagc gcggcttcct gctgccagcc
      481 cttgcacgtc agctggctgc cgcgaggagc gcgaattgtc tctgccatgc ggcggttcgt
      541 atcacgctcc tgccattctg gcgcgggttc tcggcgagct cgcgcgtcgt cgcacagttg
      601 cgcgatcgtc ttcgctggtc ttgccgttgc agtcgcctcg gcccatgacc ggttgcctag
      661 tgactctcgg aatttggatc gcgctgctcg tcggcgctgc ggcgaagctc gcccggagca
      721 gcgcgtgatc atcgcggcac tcgcggtgtg gattccggcg tggctcattt acaaccgctt
      781 gcctgccgaa gagcgactgc cgatggcgtg gctgatacgg ctgctcccat ttgtgatcgc
      841 gccgcaggtg gcgatggacc tagtgatccc atgctcggga gagcttctcg tccttcttgg
      901 tacggcgtcc gccgtgagtg aaacgctgcg gcaccggagg ctgcctcgcg cgaaggtgct
      961 caggcctggg cgagctgcat cagcatccgc aggagctcga cgcgcgtctt gacgccgagc
     1021 ttgcgcatcg cgctcgagag atgcgacgcg accgtcgatt cggcgatacc gagctcgtac
     1081 gcgatgagct tgttcgactt gccgagcgcc gcgagctgcg cgacgtctcg ctcacgagca
     1141 ccgagtgcgc gcgggtccgg gccaccgtgc tcgttgcgcc gcgcgacgat gtagcgacga
     1201 ccaccgctct cgaagtgatc gacgagcgac cagcgacccg cgacgagcgc cgtccacatc
     1261 gtcgtcgcag cttcgggatc ctcgcgacgt gcacgaccgc gtgcgcgttc ctgacgctgg
     1321 acagccgtgc gtagcgcttc gcgcgtcgag ccgtgcttcg tttccccttc ggcatgctcg
     1381 agcttgcccg atggcgtgag cactgcatcg aacgcttcgc tcgtcctcgc cgcagtcgtt
     1441 ttcgcccccg tgccggttgc tgaagccagg cgatcgcgca gccgatgtcc tgcggcgacg
     1501 tgcgcggcga ccttcgccca caaccgtctc acacgcggat cgaagcttcg gtcgcgacgt
     1561 tgtggcgcgc aaaacgcgag gcccttgccc cgtggctcca cggtgcggta cgcggccatg
     1621 tcgacggcgc cgagcttcgc gatcagacgc gcgaactcgg gatgcttgcg cggatccgcg
     1681 acgccgatct gtttgctcgt ttggccgagc gtcgtgagcg tagtcggcgc gacctggaag
     1741 cgattgacgt ccgctcgatc gtacgagccc atcatcgctt gcagcacgtc gtacagatcg
     1801 acgtcgaaat cgatcgtgac tccgctctcg agccagcgag ctggcaacgt cgtcatgtcg
     1861 aactcgtacg ccatgaagcc acggtcgcca tcgatcagcg gcgcgatgct gcgcagcaac
     1921 gcttcgagcc acggcttcat tggcgcgtcg agatcgtacg cgcgctcgac gacgtcgatc
     1981 ggattcggtg tcgcgctcat aggagatccg ccgctccgag caccagcagc gcgagctctg
     2041 cgcgcgatac gacgccgagc ttgcgataga tcgacgcgag ctggttggcg atcgtccgca
     2101 ccgcgcgttt gcgcttcttg gcgatcgccg cattcgataa acctgccgcg gcgtcacaag
     2161 cgactgcacg ttcggcggcg gtcagcgccg cgagcgtctc ggcagtgtcg gcggccgagt
     2221 ctgcgggcag gctcgtcacc acgaggcgct ctccgccgat ctcgacctcg aactgctcca
     2281 caccgcgcgg ccgcaccatc caagtacacg ataccagtgc gctcggctgg acgaaatgac
     2341 catggccacg accacacgaa cgcacctacg ctgacgcgac ctgtgccgcc tacgcgttct
     2401 tcactcgagg gtcgcgacct gtgccgctaa cgcgttcttc acgcgagggt cacggcaacc
     2461 tcgcgagtga gtcctctaac gtggttctcg tgagcatcga aaccatcgcg aatcgtctgg
     2521 cacgtgcgaa tgacttcgtt caagtcgcca tcgaggtctg cgccatgacg cgcgagctct
     2581 tcgaggtggt cgagtgtgga gtcgggcttc atgcacccgg cgggcggccg acgttggccg
     2641 tcgataatct gcggcttcca tccgacgagc cgcgcctgca atggctcgcc ggcgcgtggg
     2701 agcgggatcc gctacaccgc gagctgatcg cgatgacggc gccggtcgaa gacggtgcgc
     2761 acttcatcga attcgcacga cgtagcggct acaccggccc cacgctccac atgctgctgc
     2821 tgccgctcat ctacccccgc gggttgctcg gtcacattcg ctgcggcaag ccagcgccgt
     2881 tttccgcgga agcgcgccgc gatctccaga cgctcgcgac cgcggcctcg gtccggctca
     2941 cggagctgca cgtccagccc ggcgacgatc cgcggcttgc gcgactgaca gcgcgccaac
     3001 gtgatgtcgc acggctcgcc ggcaagggca tgagcaacgc cgagatcggg cgcacgctgg
     3061 atctgtcgca caacacgatc aagaagcact tgaaggacgt cttcgaaacg ctcggcgtga
     3121 cgagccgcgc gcagctcgcc gtgatcatgt gcgacatcgg cccggtacac gattatccat
     3181 tcggcatcac gcgcgtcgac gacatcacga tcatgcgcgg cgcgctctaa ccgagcactt
     3241 gcgatcgcta tgatgcggtg atgctcgtcg tcgccggcac gctcgtctat gcagcgctgc
     3301 agaacgacgt tctcagcgag ctcattccgc cgatggacga cctcggcttc acgcacgaca
     3361 acgtgctcgc gatcggcgtg gcgcgcggcg actacgtgat cggcggcaag ctgtttgatc
     3421 gctggatcac gtcgcgtacg gaccggcgtc gttgggatca gctcgatctc ctcgcgacgt
     3481 acgagcagcg cttgtggccc gcgggcgacg agccgtcgca atggcagctc accgcgcagg
     3541 cgcgcctcgg gcccacgttc ggcggcaact tcggcggcca gtacctgcag aatggatggc
     3601 attacatctc gaagacgggc cctaccgtcg acgaaggcct cgcgaacgac tatcggggcg
     3661 atgacaccat cggcgtcctt gccggtgccc atctgcacgc ggagagatgg ctgcggagtg
     3721 agccgtttcg cgagggcgtc gctccaagct cgccgcttcg cgcgttcgtg tattcgaacg
     3781 tcgatgggca gggcacgttc ggcgccggcg tatcgtcgat cgaaggggcg ctaggcgcgt
     3841 ggatcggcgt gcatcgcatc ggcgctcatg tcgaagcggc cgtgacgcgc taccacgtgg
     3901 cggacgcgaa cctcgcgctg cccggtggct acggcgtcgg atggcaattc gaatggcgag
     3961 ccggcatcga cgtcacgtgg agccgatacc ggctcgccta cgaataccgt gcgaacgaag
     4021 gtggctcggg tgagccgatc ggcgttgtcg ccttctcgtt gcactgacac ccgggcgttg
     4081 tagctgcctc gggccaccgc ggcaccaaat acgatcggcc aggtaggcac tgcccgaaaa
     4141 gcgaaacggg gcgctgcgaa cgccccgtcg actcagccgt ctagacgacg tctaggcttc
     4201 cttttggctc tccgccttcg cgtcggcagc tgccttggcg tcggccctca cgctctcggc
     4261 cgtcatcggc gtcgtcgcga tgcgcatgcc gtagaagctg cgatgcgcga agaacagcga
     4321 gatcaggaac gcgatgcccg cgaccgtgta cttcacgttg tcgaggccgc gctcgtgcag
     4381 ctgcagcgcg agaccgacgg cgagcacgcc gaacagcgtg gtgaacttga tcaccgggtt
     4441 cagcgcgacc gacgacgtgt ccttgaatgg atcgccgacc gtgtcaccga cgacggtggc
     4501 cgcatgcagg tccgtgccct tggcgcggag ctcggtctcg acgaccttct tcgcgttgtc
     4561 ccaagcgccg ccggcgttcg ccatgaagat cgcctgatag aggccgaaca gcgccatcga
     4621 gatcaggtag ccgatgaaga agtacggatc gaggttcgcg aacgcgagcg tcgagaagaa
     4681 gacgaccatg aagatgttga ccatgccctt ctgcgcgtac tcggtgcaga tggcgacaac
     4741 cttgttcgag tcttccgtcg acgccttcgc ctgcgcgctg gtgtcgagct tgatgttctt
     4801 cttgatgaac tcgaccgcgc gatacgcgcc cgtcgaaacg gcctgcgtcg acgcgccggt
     4861 gaaccagtag atgatcgcgc cgccgacgac gagaccgagc aagaacggcg ggtggatcat
     4921 cgacagcttg ccgacagcct caggctggag gccgttcgtg acgagcatga tgagcgcgaa
     4981 gatcatcgtc gcagcaccga caacggcggt accgatgagc accggctttg cagttgcctt
     5041 gaacgtgttg ccagcgccgt cgttctcctc gaggaacgcc ttcgccttct cgaagtcagg
     5101 gcggaacttg aagtccttct cgatctcgtc ctcgatgccg ggacgcgact cgatgagcga
     5161 aagctcgtag accgactgcg cgttgtccgt gaccggaccg tacgagtcga ctgcgatcgt
     5221 caccgggccc atgccgagga agccgaacgc gacgagaccg aatgcaaaga cggtcccggc
     5281 ggcctgcgcg gcaaacgggc tgatcgccat gtgcgcctga tcgttcgagc gcagcagcag
     5341 gtcttggata ccgatcgcgg agctgattgc atacgcgatg cccatgaggc ccgcgatgac
     5401 gagacccatc cagtatgccg agaagttacc cgccgtgaga ccggcgagga tgttgagcga
     5461 cgggccgcct tctttcgagg ccgtcaccgt ctcgcgcacg tggccagagc tcaccgaggt
     5521 gaacaccttc acgagctcgg gaatgatcgc gccggcgagc gtgccgcagg aaacgatggc
     5581 ggagagcttc caccacaggc cgtgcggcag cgtcgagtcg ccctgaccga tgaggaggta
     5641 cgagacgccg aacgtgaggc cgaccgagac gagcgacgtc agccacacga gctgcgtgag
     5701 cggatgctcg aagttcatcg tcttcgcgtt cgcgaagcgt gccttcgtga cggcctcgtt
     5761 cagcaggtag ctgccgaccg acgcaaggat catgatgatg cgcatcgcga acagccagac
     5821 gaggagcacc gtctggatcc actcgcccat gtccttgacg tcgttgccga gaagcgcggg
     5881 catgcccgga atgttcggga acgcgacgag gatgaacgtg atgagcgcga cgcccgtgac
     5941 gccgtaggtc tcgaagccgt cggccgtcgg gccaaccgag tcaccggcgt tgtcgcccgt
     6001 gcagtcggcg atgacgccag ggttacgtgc atcgtcttcc ttgatgttga agacgatctt
     6061 catgaggtcg gagccgatgt cggcgatctt cgtgaagatg ccgcccgcga tacggagcgc
     6121 tgcagcgccg agcgactcac cgaccgcgaa gccgatgaac gacgggcctg cgtagtcacc
     6181 ggggaggaac agcatgatga tgagcatcag caggagctcg acggagatga gcaccgtgcc
     6241 gatcgacatg ccggcctcga gcggaatcgc gtacgtgccg tacggcttgc cgcggagcga
     6301 cgagaacgcc gcgcgcgagt tcgcgaacgt gttcacgcga atgccgaaga acgcgacgag
     6361 cgagctgccg aggatgccga tgaccgagaa ggccgcgatg caggcgacct tcggaatcgg
     6421 tacgcgcggc gtctcgagca ccgcgaagta cacgacgatg acggccgcga tgagcaccca
     6481 gagctggaag atgaacttcg cctgcgtacg cagatacgtc ttgcaggtcg agtagatcag
     6541 ctcgctgatc tcgatcatcg ccttgtgcgc cggcaggttc ttcagcttct gatacttgat
     6601 caggccgaag agcaagccga tcgcgcagac ggccaagcca atcgcgagaa tgttccagcc
     6661 ggacattccc atgagcttca cttggtgcaa gtcagggagg gtgagctgcg cttcgctgtg
     6721 ggggcggccg ccgtgttcgg cagtgccttc cgccgctgtg gcgagccatg gaatcaggga
     6781 cgtcatgggg gttcctcggt gggtctcgtg aaggggggcg ttataaggac ggaagtctcc
     6841 ggagtgcaag gccctaaggc cccgcaaacc acgctcaagt gcgcgtttcg ttggtgcttc
     6901 cggagtcgat cgtggtatgg aacgctgccc gaaaggtttt ccacccccga tgcgccgccc
     6961 cgaaatcctg gcgcctgccg gtgaccgcga tgcgctggcg gctgcgctcg cagcaggtgc
     7021 ggacgcggtc tacttcggcc tcgacgacgg cttcaacgcg cgcgcccgcg ccgagaactt
     7081 ctcgtcgaca aacctcgcat ccatcgtcgc gcaaatccac cgcgcgggtg tccgcgccta
     7141 cgtcacgctg aacaccctcg tcttcgagcc cgagctcccg atcgtcgagg aactgatccg
     7201 ccgcgtggca gccgccggcg tggacgcgat catcgtgcaa gaccccgcgg tcgccctgct
     7261 cgcacgcgcg atctcgccgc acgcctcgtc gccgccgcac gcgtcggcgc cgaacgcatc
     7321 tcgcgttgcg agccgcgcac tcgagattca tgcgtcgacg cagatgacgg cgtcctcgcc
     7381 gctcgcgacg cgcctgctcg cgcccgcgct cgatctctct cgcgtcgtcg tgccgcgcga
     7441 gctgtccgtc gacgaaattc gcgcgtatcg cgaaggcacg aacgtcgagc tcgaagtctt
     7501 cgtgcatggc gcgctctgcg tcgcgtggag tggccaatgc ctgtcgagcg aggcgtgggg
     7561 cggccgctcg gcgaaccgcg gccaatgcgc gcaggcctgc cgcctgccct actcgctcgt
     7621 cgtcgacggc gcggcccgcg acctcggcga agtcgaatac ctcctctcgc cgaaagacct
     7681 cgtcggcctc gacgccatcg ccgacctcgc gaagatcggc gtcgcgtcgc tcaagatcga
     7741 aggccgcctc aaaggtcccg gctacgtcgc cacggcggtc gcccaatacc gccacgccgc
     7801 cgcctcctca gtcggtgaac gcgcccccac gccacccgcc ggcgtcgata tcccgctcga
     7861 ccgcgagtcc ctgcacgtcg cctacagtcg cggtgtctcg cgcggctttc tcaccggcgc
     7921 cgatcatcaa acgctcgtcg aaggtcgctt cccccgccac cgcggcctcc ccctcggccg
     7981 cgtcatcgcg atcgaaggcg acgaagtcga gctcgtccac gaccccgcgc aacgccccgt
     8041 caccggcggc ctcgcgctcg agcttgaaag ccgcacctcg gacacggcac cgaatggccg
     8101 cacctcggac accgatgacc ggtccacgga tgaccggtcc acggatgacc gttactcgga
     8161 tggacggcac ccggacgggc atgtccggct ttcggacgtc cgcgcgggca tgggcatcgt
     8221 cttcgacagt gggcggcccg aggacgagga gcaaggcggg ccgatctttg ccgtcgccga
     8281 aaccccacgt gggtatcgcg tgcg
//
LOCUS       DATLBZ010000241         5125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5477138, whole genome shotgun sequence.
ACCESSION   DATLBZ010000241 DATLBZ010000000
VERSION     DATLBZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5477138"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VMZ53_12800"
     CDS             <1..753
                     /locus_tag="VMZ53_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HUS29387.1"
                     /translation="EHGTALPTIAVGEVPRFAAGDAPYRALHARVVATSGRRVELETR
                     ESGDELWQALYASGAPVQYAHRPELLPLYAVQTAYAARPWAAEMPSAGRPLTWDVLIG
                     LRRAGVEIATLTHAAGLSSTGDDALDRALPWPERYEIPERTAAAIAETKARGGRVIAI
                     GTTVVRALESSALANGGRVVGGAGIATLRLDARYVLRVVDGLVSGLHTPGESHFDLLS
                     AFAPRERLAAAIELAAAHGLSSHELGDACLIL"
     gene            complement(750..1280)
                     /locus_tag="VMZ53_12805"
     CDS             complement(750..1280)
                     /locus_tag="VMZ53_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29388.1"
                     /translation="MKLVGMLAVLVVALLGGTARAEKTQKVTALWSPIHLVLPVVEVE
                     GEFNAAPHIGAGLILGAGRVSSEDDTVTATVFEVGAQFNYYFLRPFHGLHAGAEANYV
                     HAGDVAQDSSLTATGLELGPYVGYKVLTDIGFTFVAQVGVAFLTLQAENSSGTVMPTK
                     PKDVFPLINLNIGWSF"
     gene            complement(1291..2040)
                     /locus_tag="VMZ53_12810"
     CDS             complement(1291..2040)
                     /locus_tag="VMZ53_12810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HUS29389.1"
                     /translation="MNLLLLDPGELRSDGTCVIADRRMQHLRTVLGVEVGSTVKAGVL
                     GGALGTGEVIAIDGDGITLRLALTNSPSRPLDVELLLAVPRPKVITRTIEIAASFGVR
                     RIDLTNAWRVDKSYLRSPRLDPVALTLAARFGAEQGGTTYLPEIALHDRLMGILDARF
                     PPRELGDSTTELRLLAHPNAPAIEQVVTSRKPTVLAIGPEGGWIEREVETFVARGFQP
                     VSLGAPILRVEAAVASALGQLLLLHRLRGSS"
     gene            2071..3738
                     /locus_tag="VMZ53_12815"
     CDS             2071..3738
                     /locus_tag="VMZ53_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS29390.1"
                     /translation="MPFFQEPPRLGNQFDDDPLLPSWLARHIPEAHHGEVATEMHQLG
                     DLAVEMYAKQLADLENEPVLTQWDAWGQRIDKIEVSPLWHEAQVLAARHGMVAAGYES
                     RLGIHARTHQFAIIHVLGPSLDVYSCPLAMTDGAARTLLASGNAELAEKYVPLLTSRD
                     PSRMWTSGQWMTERTGGSDVSQSETVAKQDANGQWRLYGTKWFTSATTSNMALTLARP
                     EGNGEGSRGLALFFVELRDSNGRLRNIQVNRLKDKLGTRKVPTAELTLDGTPATMVGA
                     ASDGVRQITPMLSITRTWNAISAVSAMRRGLALARDFARRRKAFGALLVDKPLHADTI
                     AGLEAEYAGAFCLAFRSAQLIGILEHGHDDAAERLARALTPIAKLTTGKQAVAVASEA
                     IEACGGAGYIEDTGLPRILADAQVLPIWEGTTNVLSLDTLRALGKGGAFEAIMAEVES
                     CCASATDPGLAKPVEAARSAMRHATAWIQEAMPNAQRLEAGARRFAMTLGRTLELALL
                     CAHAQWCTDHPLGHGQGPRLAAAARRFAMNGVDQIADAPLDDNRALV"
     gene            complement(3795..4136)
                     /locus_tag="VMZ53_12820"
     CDS             complement(3795..4136)
                     /locus_tag="VMZ53_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29391.1"
                     /translation="MIELQGDRGKAMEQANQEMASHCGPNNYTIVQEGEEAVGTDTYT
                     REDTNAGGTSNGRSYQEGSTTQGQQSTRTATAWRVHYQCNGAGGGPAPMPAAAPGGGP
                     PPGGPPPGPGY"
     gene            4307..>5125
                     /locus_tag="VMZ53_12825"
     CDS             4307..>5125
                     /locus_tag="VMZ53_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HUS29392.1"
                     /translation="MRLEESRIEQVTVYAHGARVRRVATLRAPVPTVVRIAGLPVAVI
                     DDTVRTEAEGAGTVSSVRVGIDAPGGEARSEETAELRAARRRVAVAETEIERLRVALE
                     QLESATIIEDDPTDEPPAAWAAVLTARRSLVALRASRERTLREQQAAARRELEEARRA
                     FEVAAERDRDSSSAKAARLHEVRKYVELEVIATGDVTIHLEYQIAAARWAPSYVARLD
                     GERASFELRAVVAQDSGEDWTGVALRLSTAEPERFTQLPELHAQKIGRRQHEPAK"
BASE COUNT          823 a         1658 c         1841 g          803 t
ORIGIN      
        1 gagcacggca ctgcactacc gacgatcgcg gtgggcgagg tcccgcgatt cgcggcggga
       61 gatgcgccgt accgcgcgct gcacgcgcgg gtcgtggcga cgagcgggcg gcgtgtcgag
      121 ctcgagacac gcgagagcgg cgatgagctg tggcaggcct tgtatgcgag cggtgcgccc
      181 gtgcagtacg cgcatcggcc ggagctgctg ccgctgtatg cggtgcagac ggcgtatgcg
      241 gcgaggccgt gggccgcgga gatgccgtcg gcggggcggc cgctgacgtg ggatgtgctc
      301 atcgggctgc ggcgtgcggg cgttgaaatc gcgacgctca cgcatgcggc gggactgtcg
      361 tcgacgggag acgacgcact cgacagggcg ttgccgtggc ccgaacgcta cgagattccc
      421 gagcgcacgg cggccgcgat cgcggagacg aaggcgcggg gagggcgcgt gatcgcgatc
      481 ggaacgaccg tggtgcgtgc gctcgagtcg tcggcattgg cgaatggtgg gcgcgtcgtc
      541 ggcggcgcgg gcatcgcgac gctgcgcctc gatgcgaggt acgtgctgcg cgtcgtcgac
      601 gggcttgtga gcgggctgca tacgcccggc gagagccact tcgatctgct gagcgcgttt
      661 gctccgcgcg agcggctggc cgcggcgatc gagcttgctg cggcgcacgg gctgtcgagc
      721 cacgagctcg gcgacgcgtg cctgattctc tagaacgacc agccgatgtt gagattgatg
      781 agcgggaaga cgtctttggg cttggtcggc atcacggtgc ccgagctgtt ctccgcctgc
      841 agcgtcagga atgcgacgcc cacttgcgcg acgaacgtga agccgatgtc cgtgaggacc
      901 ttgtagccga cgtacggtcc gagctcgagg cccgtcgcgg tgagcgacga gtcctgcgct
      961 acgtccccgg cgtggacgta gtttgcttcg gcgccggcgt gcaggccgtg gaatgggcgc
     1021 aggaagtagt agttgaactg cgcgccgacc tcgaagacgg tcgccgtgac ggtgtcgtct
     1081 tcgctcgaga cgcgccccgc gccgaggatg aggcctgcac cgatgtgcgg cgccgcgttg
     1141 aattcgccct cgacttcgac gacggggagc acgagatgga tcggcgacca cagcgccgtc
     1201 accttttgcg tcttctcggc gcgagccgtt ccaccgagca gcgccaccac caataccgcg
     1261 agcatcccga cgagcttcat cgcggcgaca ctacgacgag cctcgcagcc tgtgaagcag
     1321 caacagctgg ccaagcgcag atgcgaccgc agcctcgacg cgcaggatgg gggcaccgag
     1381 tgagaccggc tggaagccgc gcgcgacgaa cgtttcgacc tcgcgctcga tccagccgcc
     1441 ttcgggtccg atcgcgagga ccgttggctt gcgcgacgtg acgacttgct cgatcgcggg
     1501 cgcgtttgga tgcgcgagta gccgaagctc ggtggtgctg tcgccgagct cgcgaggcgg
     1561 gaagcgtgcg tcgaggattc ccatcaagcg atcgtggagg gcgatttcgg ggaggtaggt
     1621 cgtgccgcct tgctcggcgc cgaagcgcgc ggccagcgtg agcgcgacgg gatcgaggcg
     1681 tggcgagcgc aggtagctct tgtcgacgcg ccaggcgttg gtgagatcga tgcgccgcac
     1741 gccgaacgac gcggcgatct cgatcgtgcg cgtgatgact ttcgggcgcg gcactgcgag
     1801 cagcagctcg acatcgagcg ggcgcgacgg cgaattcgtg agcgcgagcc gcagcgtgat
     1861 tccgtcgccg tcgatcgcga tcacctcgcc cgtgccgagc gcgccgccga ggacgccggc
     1921 cttcaccgtg gagccgacct cgacgccgag caccgtgcgc aagtgctgca tacggcggtc
     1981 cgcgatcacg caagtaccgt cgctgcgcag ctcgccgggg tcgagtaaca acaggttcac
     2041 gtcctgacct taccgcggta ggttcccgtc atgccgttct tccaggaacc gcctcgcctc
     2101 gggaaccagt ttgatgacga tccgctgttg ccgtcgtggc ttgcgcgtca cattccggag
     2161 gcccaccacg gcgaggtcgc caccgagatg catcagctcg gtgacttggc ggtcgagatg
     2221 tacgccaagc agctcgcgga tctcgagaac gagcccgtct tgacgcagtg ggatgcgtgg
     2281 ggccagcgca tcgacaagat cgaggtctcg ccgctgtggc acgaggcgca ggtcctcgcg
     2341 gcgcggcacg gcatggtggc cgcgggctac gagtcgcggc tcggcattca tgcgcgcacc
     2401 caccagttcg cgatcattca cgtgctcggg ccttcgctcg acgtctactc gtgtcctctc
     2461 gcgatgacag acggcgcggc gcgcacgttg ctcgcgagtg gcaacgcgga gcttgccgag
     2521 aagtacgtgc cgctgctgac gtcgcgcgat ccgtcgcgca tgtggacgag cgggcagtgg
     2581 atgacggagc gcaccggtgg ctccgatgtg tcgcagagcg aaaccgtcgc gaagcaagac
     2641 gcgaacgggc agtggcgcct ctacggtacg aagtggttca cgtctgcgac gacgtcgaac
     2701 atggccctca cgctcgcgcg cccggaaggc aatggcgagg gcagccgcgg cctcgcgctg
     2761 ttcttcgtcg agctgcgcga tagcaacggg cggcttcgca acatccaggt caaccgcctg
     2821 aaggacaagc tcggcacgcg caaggtgccg acggcggagc tcacgctcga cggtacgccg
     2881 gcgacgatgg tgggcgctgc gagcgacggc gttcgtcaga tcacgccgat gctctcgatc
     2941 acgcgcacgt ggaatgcgat cagcgcggtg tcggcaatgc gtcgcggcct tgcactcgcg
     3001 cgcgattttg ctcgccgtcg caaagcattc ggcgcgttgc tcgtcgacaa gccgctgcac
     3061 gccgacacga tcgcgggcct cgaagcggag tatgcgggcg cattctgcct cgcattccgc
     3121 agcgcgcagc tgatcggcat cctcgagcat ggtcacgacg atgctgccga gcgcctcgcg
     3181 cgtgcgctga cgccgatcgc gaagctgacg acgggcaagc aagcggtcgc cgtcgcatcg
     3241 gaagccatcg aagcgtgcgg cggtgccggc tacatcgagg acaccgggct gccgcgcatc
     3301 ctcgccgacg cgcaagtgct gccgatctgg gaaggcacga caaacgtgct ctcgctcgac
     3361 acgttgcgcg cgctcggcaa aggtggcgcg ttcgaagcga tcatggccga ggtcgagagc
     3421 tgctgcgcgt ctgcgacgga tccggggctc gcgaagcccg tcgaagcggc acgatcggcg
     3481 atgcgtcacg cgaccgcgtg gatccaagaa gcgatgccga atgcgcagcg cctcgaagcg
     3541 ggtgcgcgtc gctttgcgat gacgctcggc cgcacgctcg agctcgcgct gctctgcgcg
     3601 cacgcccaat ggtgtacgga ccatccgctc ggccacggcc agggaccgcg tctcgcggcg
     3661 gcggcgcggc ggtttgcgat gaacggcgtc gatcagatcg ccgacgcacc gctcgacgat
     3721 aaccgcgcgc tcgtctgacc ggccgaaacg acaaaaggcg cctcgcggcg ccctgcatcg
     3781 ttatcagctc gagctcagta gcccgggccc ggaggcggac cacccggggg aggaccacca
     3841 cccggtgcgg cggccggcat cggagcgggg ccaccgccag cgccattgca ctggtagtga
     3901 acgcgccatg cggtcgccgt gcgggtgctc tgctgaccct gcgtcgtcga gccttcctga
     3961 tacgagcggc cgttcgatgt gccacctgcg ttcgtatcct cgcgcgtgta cgtgtccgtg
     4021 ccgacggcct cttcgccctc ttgcacgatc gtgtagttat tcgggccgca gtgcgacgcc
     4081 atttcctggt tcgcctgctc catcgccttg ccgcgatcgc cttggagctc gatcacgccg
     4141 cccgcttggg tacgctgaat gacgcgcgcg ctaccacagg cagccagcgc caaaatcaaa
     4201 ccaagagtta ctgtgcgaag catgtttcct tctccgtgtg acggcccgta gacggagacg
     4261 accgtaaacc atacgctgtc gtcgttcaac aggtccttgg gtgggaatgc ggctcgaaga
     4321 atcgcgtatc gaacaggtga ccgtctatgc gcacggcgcg cgtgtgcggc gtgtcgcaac
     4381 gttgcgtgca cccgtcccaa ccgtcgttcg aatcgcgggc ttgccagtcg ccgtgatcga
     4441 cgacaccgtc cgcacggagg ccgaaggcgc aggcacggtt agctcggttc gcgtcgggat
     4501 cgacgcacct ggcggtgagg cgcgcagtga ggaaacggcg gagctgcggg cggcgcggcg
     4561 gcgcgttgcg gtagcggaga ccgagatcga gcggctgcgc gtcgcgctcg agcagctcga
     4621 gtccgccacg atcatcgaag acgatccaac ggacgagccg cctgccgcgt gggccgcggt
     4681 gctcacggcg cggcgctcgc tggtggcgtt gcgggcaagt cgcgagcgca cgttgcgcga
     4741 gcagcaagcg gctgcgcgcc gcgagctcga ggaagcacga cgcgcgttcg aagtcgccgc
     4801 ggaacgcgat cgcgacagca gcagcgcgaa ggctgcgcgc ctgcatgaag tgcgcaagta
     4861 cgtcgagctc gaggtcattg ccaccggtga cgtgacgatc catctcgagt atcaaatcgc
     4921 cgcggcgcgc tgggcgccga gctacgtcgc gcggctcgac ggcgagcgtg cgagcttcga
     4981 gctccgcgca gtcgtcgcgc aggattccgg cgaggactgg acgggcgtcg cgctgcggct
     5041 ctcgaccgcg gagcccgagc gattcacgca gctgcccgag ctacacgcgc agaagatcgg
     5101 ccgtcgccaa cacgagccgg cgaag
//
LOCUS       DATLBZ010000242        13647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2496509, whole genome shotgun sequence.
ACCESSION   DATLBZ010000242 DATLBZ010000000
VERSION     DATLBZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13647
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496509"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VMZ53_12830"
     CDS             <1..609
                     /locus_tag="VMZ53_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29393.1"
                     /translation="QAEPPGITASLGPRIPILLQHVITPFPVDGKAPGKVWGLGYEFE
                     PHDVDANTVSVAPDDEVLKIGQIGQEVEMGVELGGKIGVPQGALSALAAAPAVSLTNA
                     SLRASTSQSFQFSLHLKLTLRKLVGAGFGAGGAQWKLYRQDEPIDKPHTLLQTLLVAE
                     GTKSIKCSVRTWAKQAGFLGTSLGAKFWPYPDQAFEISLAGL"
     gene            complement(616..3051)
                     /locus_tag="VMZ53_12835"
     CDS             complement(616..3051)
                     /locus_tag="VMZ53_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HUS29394.1"
                     /translation="MGRREDFMAGLAPLQDDAVPPLHRDIKQLFANDAAFTKWEDSLW
                     LGLFFARRLTGVLANRPRVESDSPAGRIQSFLEVAQGRGAFFGPQTLLRDLPFAIRRT
                     CCVRIDGSAMGTGFLIGPDLVITNWHVVRAILKPEEHPERVSVAFDYLHEAGVGEPPI
                     SLAATKITRWRPARQEFLPGAEPSPHPLDYAVLKLAATLPGERGFFNLESTPDLATGS
                     RVVIVGHPEGKPLAVSFSRSAGLHGLDGALAYYDADTLHGSSGSLVLLESQNELKAAL
                     LHAGGKTADENFGIHLRAIHDDLQGIVSSEPAAPTKPAPKLESFFGSEPEQRAGQLAT
                     LLSVLPHDVKLDTTAPVSQQIESLEAQWDERFAEALQIARAVAVPVSDERIAEVSTHE
                     TPVAPKPEQTVGAQTARKLTTYIVMSALVVVTALIVIPKLFKKDKPPPPQATETCGIT
                     QSSYFTPQRRELECSLVPTKQPGRIQCSLHTLQGGTIHGECYHNRPSGEWSSVTPTGE
                     KLWSVDFEKGEWEVPYTYENRIKVKALERRTGADWEVESRSYPLPLGSDVVELPKQHV
                     TRIRCPKVDASAELRDGGDRVGFTIQAGNTTLDADTQHITYRGTQVASSQVDADKLST
                     QFKYLWSQIEQCHPIAIEAPSCKLGTWDAGCGNVAGPHQDCFQHLDAMKSVDLRPCGV
                     PGALEIMPPPGTPVISGDAAKALSPTCRTVLKCTAPRRPIPDLHVTPRIDPYVATLKS
                     RVTFPIGKCKSAEWDAPKKSGDKWSVTAKCLCGDGTKTPAKVVASFTNEADMPAADPL
                     TARFKLVGNQCGL"
     gene            3230..4423
                     /locus_tag="VMZ53_12840"
     CDS             3230..4423
                     /locus_tag="VMZ53_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29395.1"
                     /translation="MADVKALEKVAVRADEDLARARAYYAIANIPVDDKKIGELAAAA
                     EEAHKSLAAAVAATKPAAPAKAAPGPAAGGTLMGAETTELIATVTMRMDRLATSMAHL
                     YALRGTPLVSVRVDNKSVNRKRRVCVRCHIEGYSAEAIASAELAPNGGETFDLFPPVF
                     PEKTRQLMELTAGALHVRVDDLDSEKVELVMAKPIPMLPPTTAVLGYRDPQTGATVDQ
                     REELAAYVTPNSPEILELLRTAVDGSKMKAMIGYQSMSVDDVREQVRAIYSALAAAKI
                     TYVDTRIAFGAGAGQILQRIRLPRESIESQSANCIDGTVLMASLLEATGLDPFLVIVP
                     GHAYLAYRLQPKGGDLEYVETTVVSSKPFDEAVKIATERSSTTSSILRLDVRELRAKG
                     ITPME"
     gene            4427..8188
                     /locus_tag="VMZ53_12845"
     CDS             4427..8188
                     /locus_tag="VMZ53_12845"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HUS29396.1"
                     /translation="MAHRLVTIERKKVGLAPGQSFDIAFRPESTKNMSAFAELSADGY
                     VELRLLDASDKLLASDPLSRAPIVNYQPKAIADFKARVINRSAVTQNVVLTVTYYSEK
                     ELPTVEIETAKITSVATKVFRDELKPHVTLQGKRLKITGDPSLTSLAGKWADKELDIL
                     PNVDGLEVDRNGFKLVFEDPSDGYPNGAIVLELKCVRPITLSKLIYKVRIPDPVIRIR
                     AGLNAYGDSLHLSPVVLQLPEFQPNWVASLVEHAKSVLQDATEGLSKAVANAVAEMEA
                     NGKLRAYTQQLKQALHMQELRNFRIGASKIVLDYEPLPPEFPAPAVVTAPKPKSQRLE
                     HLVIVMLENRSFDHMMADIVAGRNDVRLAPADYSETLKDAHGIARVYKRGKTKLNALP
                     HDPAHDSTSQEIQAGGQFVRSFLEDHKGSPYGAEVLAYQPRENVAFFDFLAREFVMCD
                     DWRAALLGHTWPNRQYSLAGASRKTKKGYRDNPSGSEFEFYTIPTICDVLEAQGVDWA
                     YYKDDFAFVELYRRWVFDRTRVRTRAQFDAAIANGKLPTVTWYEPNISDFGKAVGNDD
                     HPPISVYGAQRLLANLYAQLCKLPTPNWMLAITYDESGGFYDHLRAELAPDDVEPKQG
                     FRVPAFLVSPWLAAGKVCPTRFDHTSLIRTILDNFCAPEPIFVSNKRVSNAKGFAKIL
                     ETGSGFSVDAARSMPKFTAVIPEAVSGLEMAGALEEDRVQPQHPLFAAFENAKQDLAA
                     DIAEKRAPETPGLELAGLEGAAAPPAAPAVRFEGLYIETRPVFPELAALGWTTRPMFD
                     DALEAIPPTEVSVDVAWDLVHDLRTRHPGVVIDPLWETRFPELEPDAADPDLQVAGTA
                     APQDHRWHLKAVNAEAAWKMLPGPGGKDLPGEGIVVGHLDTGYREHPDVMSMLMPAYG
                     WDVYRNDNDPHDDMEHGFLKFPGHGTGTVSIIGSRGATSDVKGTAPAVSAIPIRISAS
                     VVHISMANMVRGIQMAVERNVHIISLSAGGLWSAALHRAVRAAVDAGVIVVAAAGNYT
                     EIVVWPARFDEVICCGAINVKGADWRWSNGGRGEHVTVMAPGEDVWVLRPKSLTSIVV
                     GPGSGTSFATACTAGAMATWLAYHGRDKLIEKYGRAGLPRVAKQVLRSVTHAGSAAGV
                     LDMEKLLLAQLPAVAPAPPAPPIDPLGFAIDELQIAGTPEATIADELQFRYTVAKLSK
                     RTTAKDAGAGGVPPIDTLGALESVAAATPELVLPMSARLAIVEKKPGGN"
     gene            8188..10998
                     /locus_tag="VMZ53_12850"
     CDS             8188..10998
                     /locus_tag="VMZ53_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29397.1"
                     /translation="MGPWTRTLVTVHIVCTAEGKLTAEVTAARTPRYPRRPADPAETK
                     LPASASKDSTIASADELIPWLGSLGIDEDDLAHVRIVPFGTPAQRAALIALPWHKAIA
                     GAVVSVPRGKRPTRSQLRIPVVISSSRDKQFLDERIDSFVDATKASKTGTQGDIVLAV
                     QGDAIGRAVGTRTRLVVIETDAIGAPPDGAFNVVQLSGPPQQRVEFVQRLLRAIFHDA
                     PLDAAVLEGMRAAKLEANHVRFDLLEGDEECLRLSHALKHAATSMKHAKPFLPKNGGG
                     MPKAIPIPPPVAASPLESMAGPLDGWADHLSSTADNLDFTHEIHGANATADVLGQAGQ
                     ASDVAEAATAAADEIELDRSAQLWLEDEVGDVVAAETPLKHLGTYWLNFQIAKDIKSH
                     ATAAKLPSEELRILFEKKDKLDLDIVLYADPDKLRIEGHRFELTLQRWGATQIIRTTL
                     KPLECGIHQVRACVYYKGNMLQSLAMQVGVECASVPAHVDYTGIETFRGIMQLPQVAY
                     SLWINDTEDKHWIGVYATQDALGDLDTYDLKTPATKTVDKAVAPARKALALLEGDDGN
                     NSSYLFSPSLPTSDALTQKQHELFVELAKEGYRMFTALMQPSETPDNDYIPKLKKRLS
                     TPDQILSIARLDDESSVPWALMYDQRIDPADPGLQLCTRFQRERADGTKLVHSPADCR
                     AQADCPLASKATLRKTVCPFGFWGFRHQVELRLRNQSGGTNAVDRTRISASPKPRAAS
                     AMFEFAESKEHLKALASIIDTGEALRDRDKILDALREGNRSLYYFFCHADQDNVLHLR
                     IADGQLIEPASIAARSEAEDDGWELATWDAQRPLVFLNACESLALDAQVISPFLDKFL
                     GLGASGLVGTEVKVFTELAAGLATELLTRMVKHRESLGAALLHVRREMLGNGNPMGLA
                     YSVYGSAALHFHLDDCACGGPG"
     gene            complement(11047..>13647)
                     /gene="hrpB"
                     /locus_tag="VMZ53_12855"
     CDS             complement(11047..>13647)
                     /gene="hrpB"
                     /locus_tag="VMZ53_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HUS29398.1"
                     /translation="LVVVVDWPVGNSGIGGAENIEPQHRLALGHLLSHLPIDDVMPQL
                     VAAVKQRRTCVLVAPPGAGKTTRVPGALLDAGIIDGSIVVLQPRRLAARLAAARIASE
                     RGVQLGSEVGYQVRFDRKVSAQTRIELVTEGVLTRRLLGDPELRGVGCVIIDEFHERH
                     LDGDLALAMVARLRERRPELALVVMSATLDAEPVAAYLDGAPIVRSEGRTFPITIEHA
                     AQPDDRHLGKQVASAVRRVAQEGLDGDVLVFLPGAGEIRRCAEDLDDAARTFDLAIMP
                     LHGDLTADDQDAAVRPASRRKVILSTNVAETSVTIDGVTAVIDSGLARIARHSPWSGV
                     PSLVVEPVSKASCAQRAGRAGRTRPGRVVRLFTKHDHDLRRAFEVAEIARSDLSGIAL
                     ELHGAGLAMSELRWFEAPPAIAAQAGEDLLVRLGAVAAGTITELGRRMLRFPVHPRVA
                     RLLCEAETRGVAKEACILGGLLGQRELRLERRGRAGEAKLSSPSDLIDDLDIVLSGQR
                     EGVDRGTLFAVERATKQLERMVDTRTRSPSSDDAVDEALMIAILTAYPDRVGKRRAPK
                     SADIVFAGGGSGALAPSSAVIEPDLVVAVDVADTGARGQASRVQIRRASAVQPGWLLD
                     LYLDRIAETDELVWNAQKERVERITQMTYDGLPIDESRDVDGARRAGPAAAALLAKQA
                     LAAGIERFVDKDSLAQWRARVMFAATTAPESGLVAPTDEALAGVVARACDGLISFGEL
                     RNASLLDLLDAQLGDKRSLVNRLAPTHFTLPYRGRRAQIHYELDKPPWIASRMQDFFG
                     LARAPAIGDGRVPLVLHLLAPNQRPVQVTTDLPGFWVKHYPALRKQLMRRYPKHQWPE
                     DPTQLITGDD"
BASE COUNT         2399 a         4262 c         4649 g         2337 t
ORIGIN      
        1 caggctgagc cgccaggcat caccgcatcg ctcggcccgc gaattccgat tttgctccaa
       61 cacgtgatca cgccattccc ggtcgacggc aaggcgcccg gcaaggtgtg ggggctcggc
      121 tacgaattcg agccgcacga cgtcgatgca aacaccgtga gcgtcgcgcc cgatgacgaa
      181 gtcctcaaga tcggacagat cggccaagag gtcgagatgg gcgtcgaact gggcggcaag
      241 atcggcgtcc cccaaggcgc gctatccgct ctcgcggcgg cgccggccgt ctcgctgacc
      301 aacgcgtcac tgcgcgcatc aacgagccag agcttccaat tctcgctgca cctcaagctc
      361 acgctgcgta agctcgtcgg cgcgggcttc ggcgcaggcg gcgcgcagtg gaagctgtac
      421 cgccaggacg agccgatcga caagccgcac acactgctgc agacgctgct cgtcgccgaa
      481 ggcacgaagt cgatcaagtg ctcggtccgg acgtgggcga agcaagctgg cttcctcggc
      541 acgagcctcg gtgcgaagtt ctggccgtat ccggatcagg cgttcgagat cagcctcgct
      601 ggtctctgac cgccgctata ggccgcactg atttccgacg agcttgaacc ttgcggtgag
      661 cggatcggcc gccggcatgt cggcttcgtt cgtgaaggat gcgacgacct ttgcaggcgt
      721 cttcgttccg tctccgcaga ggcacttcgc ggttacgctc cacttgtctc ccgacttctt
      781 cggcgcgtcc cactccgcgc tcttgcactt gccgatcgga aacgtgacgc gcgacttcag
      841 cgtcgcaacg tacggatcga tgcgcggtgt cacgtgaaga tcaggaatcg gccggcgtgg
      901 cgcggtgcac ttcagtacgg tcctgcacgt cgggctcagc gccttggccg cgtcaccgct
      961 gatcacgggc gtgccgggcg gaggcatgat ctcgagcgca ccgggaacgc cacacggccg
     1021 caggtcgacc gacttcattg cgtcgagatg ctggaagcag tcctggtgcg gcccggcgac
     1081 gttcccgcag cccgcgtccc acgttccgag cttgcacgac ggagcctcga ttgcgatcgg
     1141 atgacattgc tcgatctgcg accacagata tttgaactgc gtgctcagct tgtctgcgtc
     1201 gacttgtgag ctcgcaacct gcgtgccgcg atacgtgatg tgttgcgtat cggcatcgag
     1261 cgtcgtgttg ccggcttgga tcgtgaagcc cacgcggtcg ccaccgtcgc gcagctcggc
     1321 ggatgcatca accttcggac agcggatgcg ggtgacatgt tgtttcggca gctcgacgac
     1381 gtcggagccc agtgggagcg gataggagcg cgactcgacc tcccaatcgg cgccggtgcg
     1441 acgctcgagc gctttgacct tgatgcggtt ctcgtacgtg tacgggactt cccactcgcc
     1501 cttctcgaag tcgacgctcc atagcttctc gcccgtcggc gtcaccgacg accattcgcc
     1561 cgacggtcgg ttgtgatagc actcgccgtg aatcgtgccg ccttgcagcg tgtgcaggct
     1621 gcattggatg cggcctggct gtttcgtcgg gacgaggctg cactcgagct cacgccgctg
     1681 cggcgtgaag taactgctct gcgtgatgcc gcacgtctcg gttgcttgcg gcggtggtgg
     1741 cttgtccttc ttgaagagct tggggatcac gatcagggcc gtgaccacca cgagcgcgct
     1801 catgacgatg tacgtcgtca gcttgcgcgc cgtttgcgcg cctacggtct gctcgggctt
     1861 cggtgcgacg ggcgtttcgt gcgtcgatac ctcggcgatg cgctcgtccg acaccggcac
     1921 ggcgaccgcg cgcgcgatct gtagcgcttc ggcgaaccgc tcatcccact gcgcttcgag
     1981 gctctcgatc tgctgagaga ccggcgcggt cgtgtcgagc ttcacgtcgt gcggcaatac
     2041 gctgagcagc gtcgcgagct ggccagcacg ctgttcgggc tcggagccga agaagctctc
     2101 gagcttcggc gctggcttcg tcggcgccgc gggttccgag gacacgatgc cttgcagatc
     2161 gtcgtggatc gcgcgcaggt ggatgccaaa gttctcgtcg gcggtcttgc cgcccgcgtg
     2221 cagcagcgcc gctttcagct cgttctgact ctcgagcaac acgagcgagc ctgacgatcc
     2281 gtgcagcgta tcggcatcgt agtacgcgag cgcaccgtcg aggccgtgca ggcccgcgct
     2341 gcgactgaac gagacggcaa gcggcttgcc ctcgggatgg ccgacgatga cgacgcgact
     2401 gcccgtcgcg agatcgggcg tgctctctag attgaagaag ccgcgctcgc ccggcagcgt
     2461 ggccgcgagc ttgagcacgg cgtagtcgag cggatgcggg cttggttcgg cgccgggcag
     2521 aaactcttgc cgcgcgggtc gccaccgcgt gatcttcgtc gccgcgagcg agataggagg
     2581 ctcgccaact ccagcttcat ggaggtagtc gaacgcgaca ctcacgcgct ccgggtgttc
     2641 ttcgggcttg aggatcgcgc ggacaacgtg ccagttcgtg atcacgagat ccgggccgat
     2701 gagaaatccc gtccccatcg ccgagccatc gatgcgtacg caacatgtgc ggcggatcgc
     2761 gaacgggaga tcgcgcagta gcgtctgcgg gccgaagaac gcgccgcggc cttgcgcgac
     2821 ttcgaggaaa gactggatgc gacctgcggg cgagtcagac tcgacgcgcg ggcggtttgc
     2881 gagcacacca gtcaggcgcc gcgcgaagaa cagcccgagc cagaggctgt cttcccactt
     2941 cgtgaatgca gcgtcgtttg caaagagctg cttgatgtcg cggtgcagcg gtggaacggc
     3001 gtcgtcctgc agtggcgcaa gacccgccat aaagtcttcg cgtcgaccca tgcttccccc
     3061 actgcattag atcgcatctt gcgccgctgt gtgcggcgtc gagtgcaaca aattgctgcg
     3121 gccgcgcgac aacacctctc gtcgatcgtt gataggctga tcgacctagg gtggacgctc
     3181 tgtcgtgagc gcagggcgaa cgtgtgcgct cgattcaatg ggggacgaca tggctgatgt
     3241 gaaggcgctc gagaaagtgg cggtgcgcgc agatgaggat ctcgcgcgtg cacgcgcgta
     3301 ctacgcgatc gcgaacattc ccgtcgacga caagaagatc ggcgagcttg ctgcggcggc
     3361 ggaggaagcg cacaagtcac tcgctgcggc agtcgccgcg acgaagcctg cggcacccgc
     3421 gaaggctgcg ccgggaccag cggctggcgg cacgctcatg ggcgcggaga cgaccgagct
     3481 aatcgcgacc gtgacgatgc gtatggaccg gctggctacg agcatggcgc atctctatgc
     3541 actgcgcggc acgccactcg tgagcgttcg cgtcgacaac aagtcggtga atcgcaagcg
     3601 ccgcgtttgc gtgcgctgtc acatcgaggg ctactccgca gaagcgatcg caagcgccga
     3661 gctcgcgccg aatggtggtg agaccttcga tttgttcccg cccgtattcc cggagaagac
     3721 gcggcagctg atggagctga ccgcgggcgc gctgcacgtg cgcgtcgacg acctcgacag
     3781 cgagaaggtc gagctcgtga tggcgaagcc gattccgatg ctgcctccaa cgacggcagt
     3841 gctcggctac cgcgatccgc agaccggcgc gacggtggat cagcgcgagg agctcgctgc
     3901 ctacgtgacg ccgaactcgc cggagattct cgagctgctg cgcaccgcag tggatggctc
     3961 gaagatgaag gcgatgatcg gatatcagtc gatgtctgta gatgatgtgc gcgagcaagt
     4021 gcgcgcgatc tacagtgcgc tggcggcggc gaagatcacg tacgtcgaca cgcgcatcgc
     4081 attcggggcg ggggcgggcc agatcctgca gcggattcgc ctgccgcgcg aaagcatcga
     4141 gagtcagtca gcgaattgca tcgacgggac ggtgctgatg gcgagcttgc tcgaagcgac
     4201 cggcctcgat ccgttcctcg tgatcgtgcc tggccacgcg tatctcgcgt accggctgca
     4261 gccgaagggc ggcgacctcg aatacgtcga gacgaccgtg gtctcgagca agcccttcga
     4321 cgaggccgtc aagatcgcga cggagagatc gagcacgacg tcttcgattc tgcgcctgga
     4381 tgtccgcgag ctgcgggcca agggcattac cccgatggag tgagccgtgg cgcaccgtct
     4441 agttacgatc gagagaaaga aagtcgggct ggcacccggc cagtcgttcg acatcgcgtt
     4501 ccgcccagag tcgacgaaga acatgtcggc gttcgcggag ctgtccgcgg atggatatgt
     4561 cgagcttcgg ttgctcgatg cgagtgacaa gctgctcgcg agcgatccgc tatcgcgcgc
     4621 gccgatcgtc aattaccagc cgaaggccat tgctgacttc aaggcgcgcg tcatcaatcg
     4681 ctcggcggtg acgcagaacg tggtcttgac ggtcacgtac tacagcgaga aggagctgcc
     4741 gaccgtcgag atcgagacag cgaagatcac gtcggttgcg acgaaagtct ttcgcgatga
     4801 gctgaagccg catgtcacgc tgcaggggaa gcggctgaag attacgggcg atccgtcgtt
     4861 gacgtcgctt gcgggcaagt gggcggacaa ggagctcgac atcctgccca atgtcgacgg
     4921 gctcgaggtc gatcggaatg gattcaagct cgtgttcgag gatccgtcgg atggatatcc
     4981 gaacggcgcg atcgtgctgg agctcaagtg tgttcggccg atcacgctga gcaagctcat
     5041 ctacaaggtt cgcattccgg atcctgtcat tcggatccgc gcgggactga acgcgtatgg
     5101 cgactcgctg catctgtcgc ccgtcgtgct gcagctgccg gagtttcagc cgaattgggt
     5161 agcgtcactc gtcgagcacg cgaagagcgt cttgcaggat gcgacggagg gactgtcaaa
     5221 ggcagtcgcg aatgcggtcg ccgagatgga ggccaacggg aagctgcgcg cgtacacgca
     5281 gcagttgaag caggcgctcc acatgcagga gctccgcaac ttccgcatcg gtgcgtcgaa
     5341 gatcgtgctc gattacgagc cgctgccgcc ggagtttcct gcgcctgccg ttgtgacggc
     5401 gccgaagccg aagtcgcagc ggctcgagca cctcgtgatc gtgatgctcg agaatcgttc
     5461 gttcgaccac atgatggctg acatcgtcgc gggacggaac gacgtgcgac tcgcgcccgc
     5521 ggactacagc gagacgctga aggacgcgca tggcatcgcg cgcgtgtaca agcgcggcaa
     5581 gacgaagctg aacgcgctgc cgcatgatcc cgcgcacgat tcaacgagcc aggagattca
     5641 ggcgggcggc cagttcgtgc gcagcttttt ggaggatcac aagggaagcc cgtacggcgc
     5701 ggaggtgctc gcgtatcagc cgcgcgagaa cgtcgcgttc ttcgacttct tggcgcgcga
     5761 gttcgtgatg tgtgacgact ggcgagcggc gttgctcggt catacgtggc cgaatcgcca
     5821 gtattcactc gcgggcgcgt cgaggaagac gaagaagggc taccgcgaca acccgtcggg
     5881 atcggagttc gagttctata cgattccgac gatctgcgat gtgctcgagg cgcagggcgt
     5941 cgattgggcg tactacaagg acgacttcgc gttcgtcgag ctgtaccggc gctgggtgtt
     6001 cgaccgcacg cgagtgagga cgcgcgcgca gttcgatgcg gcgatcgcga acggcaagct
     6061 gccgaccgtg acctggtacg agccgaacat ttccgacttc ggcaaggccg tcggtaacga
     6121 cgaccatcca ccgatcagcg tgtatggcgc gcagcggttg ctcgcaaatc tgtatgcgca
     6181 gctgtgcaag ctgccgacgc cgaactggat gcttgcgatc acctacgacg agagcggcgg
     6241 cttctacgat caccttcgtg cagagctcgc gcccgacgat gtcgagccca agcagggctt
     6301 ccgcgtgccg gcgttcctcg tctcaccctg gttggcggcc gggaaagtgt gtccgacgcg
     6361 cttcgatcac acgtcgctca ttcgcacgat cctcgacaac ttttgcgcgc cggagccgat
     6421 cttcgtcagc aacaagcggg tgtctaacgc caagggcttc gcgaagatcc tcgagacggg
     6481 ctccggcttt tcggtcgacg ccgcgcgctc gatgccgaag ttcaccgcgg tcattcccga
     6541 ggcagtcagc gggctcgaga tggcaggcgc actcgaggaa gatcgcgtgc agccgcagca
     6601 tccgctgttt gccgcgttcg agaatgcgaa gcaggaccta gcggcggaca tcgcggagaa
     6661 gagagcgccg gagacgccgg ggctcgagct cgctgggctc gaaggtgctg cggcgccacc
     6721 cgctgcgccg gccgtgcgat tcgaggggct ctacatcgag acgcgtcctg tattccccga
     6781 gctagctgcc ctcggctgga cgacgcggcc gatgttcgac gacgcactcg aggcaattcc
     6841 gccgacggag gtgtctgtcg atgtggcgtg ggatctcgtg cacgaccttc gcacgcggca
     6901 tccgggtgtc gtgatcgatc cgttgtggga aacgcggttc ccagagctcg agcccgacgc
     6961 tgccgatcct gacctccagg tcgccggcac tgctgctccg caggatcatc gctggcactt
     7021 gaaggcggtc aacgctgagg cggcgtggaa gatgctgccg ggcccgggcg ggaaggacct
     7081 ccccggagaa ggaatcgtcg ttggacatct cgataccggc taccgcgagc acccggatgt
     7141 gatgtcgatg ctcatgccgg cgtatgggtg ggatgtctat cgcaacgaca acgatccgca
     7201 cgacgacatg gagcacgggt tcttgaagtt cccgggccac ggaacgggga ccgtgagcat
     7261 cattggcagt cgtggtgcca cgtcggacgt gaagggtacg gcgcccgccg tctcggcaat
     7321 tccgattcgc atctccgcat cggtcgtgca catctcgatg gcgaacatgg tgcgcgggat
     7381 ccagatggcc gtggagcgca acgtccacat catctcgctc agcgcgggtg ggctgtggtc
     7441 tgcggcgctg catcgcgcgg ttcgcgcggc agtcgatgcg ggcgtgatcg tcgtcgcggc
     7501 cgcaggcaac tacacggaga tcgtcgtgtg gcccgcacgg ttcgacgagg tcatctgctg
     7561 tggcgcaatc aacgtaaagg gcgccgattg gcggtggtcc aacggcgggc gtggcgagca
     7621 cgtcaccgtg atggcgccag gcgaggacgt ctgggtgctg cggccgaaga gcttgaccag
     7681 catcgtcgtc ggaccaggct cggggacgtc gtttgcgacg gcatgtacgg cgggcgcgat
     7741 ggcgacatgg ctcgcgtatc acggtcgcga caagctgatc gagaagtatg gtcgcgctgg
     7801 gctgccccgc gtcgcaaagc aggtgctgcg cagcgtgacg cacgcgggct cggcggcagg
     7861 cgtgctcgac atggagaagc tgctgctagc gcagttgccc gccgtggcgc ccgcaccgcc
     7921 ggctccaccg atcgatccgc tcggcttcgc gatcgacgag ctgcagatcg cagggacgcc
     7981 ggaagcgacg atcgccgacg agctgcaatt ccgctacacc gtcgcaaagc tatcgaaacg
     8041 gacgaccgcg aaggatgcag gcgcgggcgg tgtgccgccg atcgatacgc tcggtgcgct
     8101 cgagagcgtc gcggctgcga cacccgagct ggtgttgccg atgtccgcgc gccttgccat
     8161 cgtggagaag aagcccgggg ggaattgatg gggccttgga cgcgcacgct cgtcaccgtt
     8221 cacattgtct gtacggccga aggcaagctg accgcagaag tcacagctgc gcgaacgccg
     8281 cgctatccgc gacgtcctgc cgatccagcg gaaacgaagc tccccgcgtc ggcgagcaag
     8341 gacagcacga tcgcgtctgc ggacgagctg attccgtggc tgggctcgct cggcatcgac
     8401 gaggacgacc tcgctcacgt gcgcatcgtt ccgttcggta cgccggcgca acgcgcggca
     8461 ctgatcgcgc tgccgtggca caaggccatc gccggcgccg tcgtgagcgt cccgcgcggt
     8521 aagcgaccga cgcgctcgca gcttcgtatc ccggtggtca tctcgtcgag ccgggacaag
     8581 caattcctcg acgagcgcat cgattcattt gtcgacgcga ccaaggcgtc gaagaccgga
     8641 acgcagggag acatcgtgct cgccgtgcag ggtgatgcga ttggccgcgc agtcggcaca
     8701 cgcacgcgcc tcgtcgtgat cgagacggat gcgatcggag cgccgccgga tggcgcgttc
     8761 aatgtcgttc aactgtcggg cccgccacag cagcgtgtcg aattcgtgca gcgcctgctg
     8821 cgcgcgatct ttcacgatgc gccgctggac gccgcggtcc tcgagggaat gcgtgccgcg
     8881 aagctcgaag cgaaccatgt gcgcttcgat ctactcgagg gagatgagga gtgcctgcgg
     8941 ctgagccacg cgctgaagca cgccgcgacc agcatgaagc acgcgaagcc gttcctgccg
     9001 aagaatggcg gcggcatgcc gaaggcgatt ccgattccac cgccggtcgc tgcgtcgcct
     9061 ctggagagca tggccggtcc gctcgacggt tgggccgacc atctctcgtc gacggccgac
     9121 aacctcgact tcacccacga gattcacggc gcgaacgcga ccgccgacgt gctgggccag
     9181 gccggacagg catccgatgt cgccgaggct gcgaccgcgg ccgccgacga gatcgagctc
     9241 gatcgatcgg cgcagctgtg gctcgaggac gaggtcggcg atgtcgtcgc agccgagacg
     9301 ccgctgaagc atctcggcac ctactggctc aacttccaga tcgcgaagga catcaagtcg
     9361 cacgcgacgg cggccaagct accgagcgaa gagctccgca tcttgttcga gaagaaggac
     9421 aagctcgatc tcgatatcgt cctgtatgca gatccggaca agctgcgcat cgaaggtcat
     9481 cgcttcgagc tcacgctcca gcgatggggc gccacgcaga tcattcgcac gacgctgaag
     9541 ccgctcgagt gcggcattca ccaggtgcgc gcgtgcgtct attacaaagg caacatgctc
     9601 cagtcgctcg cgatgcaagt gggcgtcgaa tgcgcgagtg tgccggcgca tgtcgattac
     9661 accggcatcg agacgttccg cggaatcatg cagctgccgc aggtcgcgta ctcgctgtgg
     9721 atcaacgaca ccgaggacaa gcactggatc ggcgtgtacg cgacgcagga tgcactcggt
     9781 gatctcgata cgtacgatct caagacaccg gccacaaaga ccgtcgacaa ggcagtcgcg
     9841 cccgcacgca aggcgcttgc gctattggag ggcgatgacg gcaacaactc gtcgtacctg
     9901 ttctcgccat cactgcccac gagcgacgcg ctgacgcaaa aacagcacga gctctttgtc
     9961 gagctcgcga aggaaggcta ccggatgttc acggcgctga tgcagccgtc ggagacgccg
    10021 gataacgact acatcccgaa gctcaagaag cgccttagca cgcccgatca gattctctcg
    10081 atcgcgcgtc tcgatgatga atcgagcgtg ccgtgggcgc tcatgtacga ccagcgcatc
    10141 gatcccgcgg atcccggact gcagctgtgt acgcgctttc agcgagagcg cgcagacggc
    10201 acgaagctcg tgcattcgcc cgccgactgc cgagcacaag ccgactgccc actcgcgagc
    10261 aaggcgacgc tgcgcaaaac ggtttgcccg ttcggattct ggggcttccg ccaccaggtc
    10321 gagcttcgct tgcggaacca atcgggtgga accaacgccg tcgaccgcac tcgaatctct
    10381 gcatctccga aaccgcgcgc cgcgagcgcg atgttcgagt tcgcagaatc gaaggaacac
    10441 ctcaaagctc tcgcgtcgat catcgataca ggtgaagcgc tccgcgaccg cgacaagatt
    10501 ctcgatgcgc tgcgcgaggg aaatcgcagt ctctattact tcttctgcca cgcggatcaa
    10561 gacaacgtcc tgcacctacg catcgccgat ggccagctca tcgagccggc atcgatcgcg
    10621 gcacgttcgg aagccgagga tgacggctgg gagctcgcga cgtgggacgc gcaacgcccg
    10681 ctcgtctttc tcaacgcgtg tgaaagcctc gcgctcgatg cgcaggtgat ctcgccgttc
    10741 ctcgacaagt tcttgggcct cggtgcaagc gggttggtcg gaaccgaagt gaaggtattc
    10801 accgagctcg cagccggcct cgcgaccgag ctgttgacgc gaatggtgaa acaccgcgaa
    10861 tcactcggtg ccgcgctcct ccacgttcgc agagagatgc tcggcaacgg caatccgatg
    10921 ggcctggcgt actccgtcta cggatctgcg gcgctgcact ttcatctcga tgactgcgcg
    10981 tgcggaggcc ccggatgacc gcgtgcaggt ggtgcatgca ggtcggtaga gcaagcgcga
    11041 tgccgatcag tcgtcgcccg tgatgagctg cgtcggatct tcgggccact ggtgtttcgg
    11101 atagcgacgc atgagctgct tgcgcagtgc agggtagtgc ttgacccaga agccgggcag
    11161 gtcggtcgtg acttgcaccg ggcgttggtt cggcgcgagg aggtggagga cgagcgggac
    11221 gcgaccgtcg ccgatcgctg gagcgcgcgc gaggccgaag aagtcctgca tgcgcgatgc
    11281 gatccatggc ggcttgtcga gctcgtagtg gatctgcgcg cggcggccgc ggtacggcag
    11341 cgtgaagtgc gtgggcgcga gacggttgac gagcgagcgc ttgtcgccga gctgagcgtc
    11401 gagcagatcg aggagcgagg cgttgcgcaa ctcgccgaac gagatgaggc cgtcgcatgc
    11461 gcgcgcgacg acgccggcaa gcgcttcgtc ggtcggcgcg acgaggccgg attcgggcgc
    11521 cgtggtcgcc gcgaacatca cgcgcgcgcg ccattgggcg agggagtctt tgtcgacgaa
    11581 gcgttcgatg ccggcggcga gggcttgttt ggcgagcagc gcggcggcgg cgggacctgc
    11641 gcggcgtgcg ccatcgacgt cgcgcgattc gtcgataggg aggccgtcgt aggtcatttg
    11701 cgtgatgcgc tcgacgcgtt ctttctgcgc gttccacacg agctcgtcgg tttcggcaat
    11761 gcgatcgagg tagaggtcga gcagccagcc cggttgaacg gcggacgcac ggcggatttg
    11821 cacgcgagag gcttggccgc gcgcgcccgt gtcggcgaca tcgactgcga cgacgaggtc
    11881 gggctcgatg acagcggacg atggcgcgag tgcgccgctg ccaccgcctg cgaagacgat
    11941 gtcggcagac ttgggggcgc gccgcttgcc gacgcgatcg gggtacgcgg tcaggatcgc
    12001 gatcatcagc gcttcgtcga cggcgtcgtc agaagagggc gaccgcgtgc gggtgtcgac
    12061 catgcgctct agctgcttcg tcgcgcgctc gacggcgaac agcgtgccgc gatcgacgcc
    12121 ttcgcgctgg ccggagagca cgatatcgag atcgtcgatg aggtcagacg gcgacgagag
    12181 cttcgcttcg ccggcgcggc ctcgccgttc gagacgcagc tcgcgctggc cgagcaggcc
    12241 gccgaggatg caggcctcct tcgcgacgcc acgcgtttcg gcttcgcaga ggaggcgagc
    12301 gacgcgcggg tggactggaa accgcaacat gcggcgaccg agctccgtga tcgtgccggc
    12361 ggcgacggcg ccgaggcgaa cgagcaggtc ttcgccggct tgggccgcga tcgccggcgg
    12421 cgcttcgaac cagcgcagct cgctcatcgc gagaccggct ccatggagct cgagcgcgat
    12481 gccggagaga tcgctgcgcg cgatttcggc gacctcgaat gcgcggcgca gatcgtgatc
    12541 atgcttcgtg aacagacgca ccacgcgacc tgggcgtgtg cggcccgcgc gaccagcgcg
    12601 ctgcgcgcag ctcgctttgc tgacgggctc gacgacgagc gatgggacgc cggaccacgg
    12661 cgagtggcga gcaatgcgcg cgaggccgct gtcgatcacc gcggtgacgc cgtcgatcgt
    12721 gacggaggtt tcagcgacgt tcgtcgagag gatcaccttg cgacgcgagg cggggcgcac
    12781 cgctgcgtcc tgatcgtcgg cggtgaggtc gccgtgcagc ggcatgatcg cgagatcgaa
    12841 cgtgcgcgcg gcgtcgtcga gatcttcggc gcaccggcgg atctcgcccg caccggggag
    12901 gaacacgagc acgtcgccgt cgaggccttc ttgcgcgacg cgccggaccg cggaggcgac
    12961 ctgcttgccg aggtgacggt cgtccggctg tgcggcgtgc tcgatcgtaa ttgggaatgt
    13021 gcggccttcg ctgcggacaa tcggcgcgcc gtccagatac gccgcgaccg gctctgcatc
    13081 gagcgtcgcg gacatcacga cgagcgcgag ctcgggtcgg cgttcgcgca ggcgcgcgac
    13141 catcgcgagt gcgaggtcac cgtcgagatg tcgctcgtgg aactcgtcga tgatcacgca
    13201 gccgacgccg cgcagctctg gatcgccaag cagccgtcgc gtcagcacgc cttcagtgac
    13261 gagctcgatc cgcgtctgcg cgctgacctt gcgatcgaag cgaacttgat agccgacctc
    13321 gctgccgagc tgcacgcctc gttcactcgc gatccgcgcc gcggccaacc gggcggcgag
    13381 ccggcgaggt tggagcacga cgatgctgcc atcgatgatg cccgcgtcga gcagagcgcc
    13441 gggtacgcgc gtcgtcttgc ctgcgccggg aggcgcgacg agcacgcacg ttcgccgctg
    13501 tttcaccgcg gcaacaagct gcggcatcac atcgtcgatc ggcaggtgtg acagcaagtg
    13561 gcccaaagct agcctatgct gcggctcgat gttctccgcg ccgccgatgc cagagtttcc
    13621 gacgggccag tcgaccacga cgaccag
//
LOCUS       DATLBZ010000243        11277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1006254, whole genome shotgun sequence.
ACCESSION   DATLBZ010000243 DATLBZ010000000
VERSION     DATLBZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006254"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VMZ53_12860"
     CDS             complement(<1..193)
                     /locus_tag="VMZ53_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HUS29399.1"
                     /translation="MSRDRRVLPHNLDAEASILGGIILRNEILLQLETLETDDFYHHQ
                     HKVVFEAIRNLEATAKPIDV"
     gene            complement(233..505)
                     /locus_tag="VMZ53_12865"
     CDS             complement(233..505)
                     /locus_tag="VMZ53_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29400.1"
                     /translation="MKPEAVPAPTSCVALMPRIRAAVVAYDASYTMLVDHALPTILAS
                     CLNDDWPLELRTCIAQSNPDALAHEHACEKLVPVELEKKLRARLTP"
     gene            complement(550..1005)
                     /gene="rplI"
                     /locus_tag="VMZ53_12870"
     CDS             complement(550..1005)
                     /gene="rplI"
                     /locus_tag="VMZ53_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HUS29401.1"
                     /translation="MAMQIILTQDVENLGKAGELVSVRPGYGRNYLVPRGMAVTATVK
                     NKNRLDHEKALIERRVAKERANATDIAARINGMTLQFERQTGEDEKLFGSVTSRDIAD
                     QLKKANVEIDHRWVALDGAVKALGKYEVPVKLAAGVTATLKFWVVGKDK"
     gene            complement(1008..1304)
                     /gene="rpsR"
                     /locus_tag="VMZ53_12875"
     CDS             complement(1008..1304)
                     /gene="rpsR"
                     /locus_tag="VMZ53_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HUS29402.1"
                     /translation="MSTRMDAGMGDDGLDRDGDDRRGGKGARGATRRKLLSDEIFQKV
                     DYKNPQVLRSFVTDRGKMIPRRISGASARQQRVISTAIRRARMLALLPFSVTGD"
     gene            complement(1308..1835)
                     /gene="rpsF"
                     /locus_tag="VMZ53_12880"
     CDS             complement(1308..1835)
                     /gene="rpsF"
                     /locus_tag="VMZ53_12880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HUS29403.1"
                     /translation="MARLQSLRDTPNTAREYETIYILRPNTPNEGVAEVNTRIKGIIE
                     GMGGKIIKVDNWGKRRLAYEVAKERKGIYLYWQYLAQPGVVEETERNLRMLDSVIRYL
                     TTKVDTDIDVAARPSELDDSSFEKAAQTAADEEDLFLSRGTDDSSSSDDDDDDYSDDF
                     ADEPVSGNVPTTEKE"
     gene            complement(1907..2515)
                     /gene="pth"
                     /locus_tag="VMZ53_12885"
     CDS             complement(1907..2515)
                     /gene="pth"
                     /locus_tag="VMZ53_12885"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HUS29404.1"
                     /translation="MWLVVGLGNPGSKYARNRHNVGFMVVDELAKKHALPAWKASVKG
                     GEATTGLVTTERGREKAVLLKPLEFMNLSGFAVSRAAQFYSVEPDKILVIHDEIDLDF
                     GIVRLKNGGGHGGHNGLRSIMEQLGGGAFARLRMGVGRDPNKPPGSKDAASWVLADFP
                     SAQAAALERVISAGCEDTEATLGLGIVPAMNKHNARSNLASS"
     gene            complement(2591..3388)
                     /locus_tag="VMZ53_12890"
     CDS             complement(2591..3388)
                     /locus_tag="VMZ53_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HUS29405.1"
                     /translation="MATSTEVKKPEIGKLTVALRDSSGKGAAIKLRQQGKVPGVCYGA
                     SPDGRIEPVSIVVEVKALRAALDPVRKQNTVIDLTIEGGGQAQKLHALVKEYQLHPLR
                     REITHVDLLAIDPNKEVNANVPLEFVGKPKGAVDGGMIRIVIRALEVRAKPSDIPVKL
                     TVDVSPLEIGDVIHVNAIALPTGVTAVTGRDLAVVTCAKPEEEKVETPAAGDAAAAGA
                     AAGAAPAAGAAPAAGAAPAAGAKDAKAAPAAAGAKDAKAAAAPAKKK"
     gene            complement(3407..4354)
                     /locus_tag="VMZ53_12895"
     CDS             complement(3407..4354)
                     /locus_tag="VMZ53_12895"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HUS29406.1"
                     /translation="MLKSLSIFTGNANRALADEICKFVEIPLGRADVTHFSDGEIYVE
                     IGENVRGVNCFVVQPTCSPPNQSLMELLIMIDALKRASAGSIVAIMPYFGYARQDRKA
                     KPRTPITSKLVANLITAAGADRALAMDLHAGQIQGFFDIPFDHLFAMPVLIDELRVLG
                     FGGDSTVVVSPDAGGVERARAFSKRLGASLAIIDKRRPAPNVSEVMNIVGDVKGKKAV
                     IVDDIIDTAGTLTSAAHAIMNAGAASVSSCATHPVFSGKAVPRITESPLQNVVVTNTI
                     PLSEAGQECSKVRVKSVGRLLGEAIKRIHHGDSISSLFV"
     gene            complement(4447..4674)
                     /locus_tag="VMZ53_12900"
     CDS             complement(4447..4674)
                     /locus_tag="VMZ53_12900"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HUS29407.1"
                     /translation="MPLAPELLETLVCPKSKQKLVYFEAEQKLVCPASRLSYRIDRGI
                     PVLLVDEATELSQPEVDRFVAKAKELGLPNA"
     gene            complement(4779..6275)
                     /locus_tag="VMZ53_12905"
     CDS             complement(4779..6275)
                     /locus_tag="VMZ53_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS29408.1"
                     /translation="MNRLFRPTIAALGLAAAATVAPSTASANVEIGGTAGIHVFSTTN
                     ELGVPDVDDAPSERNSALFGLRLGVTFNDMLGVEGEVGVIPSETRGMSLADIWNVTYR
                     AHLIAQFGAADPAKKLVPFALVGGGATSVIDSKNEDLVEKDTDAAMYIGVGAKYRVDN
                     GWGLRADARLLFPPSSSGNGPTLDFEALLSIYKEFGRKVVEKVVETPKGPNDTDGDGL
                     TDDVDKCPNEAEDKDGFQDDDGCPDLDDDGDGVPDATDKCKMEPEDKDSFQDEDGCPD
                     PDNDADGIPDTADKCATEPEDKDNFQDEDGCPDPDNDGDGVLDGADKCIDQPETKNGY
                     QDEDGCPDEIPQKVKQFTGAIQGINFKTSSAELLPTSNKTLDKAVAVLKEFSDLKLQI
                     EGHTDDVPLKPKAGSQFQDNKSLSQGRADAVKAYFVKAGIEESRLTAIGFGPDKPAVD
                     PTGLKGGKLKAAQAKNRRVEFTLISNLVPGNGAAPAPAQGGSAMPTPP"
     gene            6402..7508
                     /locus_tag="VMZ53_12910"
     CDS             6402..7508
                     /locus_tag="VMZ53_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HUS29409.1"
                     /translation="MQDARAQLQAMLRPSAGGLYVVSTGRAAQLEVQKHLYGTDDESQ
                     IHARFTAALDRIATARAIILGVPSDVGAGYRRGANLGPQAIRSALIAADPDLRASFDK
                     LGVVDIGDVAVVPQLLHDSMLSTQQIEASRAALYKDAPPALAAQLPVSPLSIEARVLD
                     LVLAINPRVMPIVLGGDHSVAWPVAEALAKHRTDRWSIVQPDAHTDLLSERLGIKYCY
                     ATWSYHANELLGRDGRLVQVGVRASRFQREHWESTLGVRQFWASEIEADRERALDDVI
                     AHLKARNVTGVYFSNDIDGTDAKFAEATGTPEPDGLDPDWLLALIARLGKEIGLIAAD
                     CVEVAPPLGPTPQATDATTRLAARYVRACLDQLR"
     gene            complement(7525..7902)
                     /locus_tag="VMZ53_12915"
     CDS             complement(7525..7902)
                     /locus_tag="VMZ53_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29410.1"
                     /translation="MKSLKVAASKPKWRAEVFLDATGGASNVYTSTVDTVFRLTVFAD
                     EWNLLVSKPGAATSIRVHDTVELDDQLKVVKGKSPPPLSELGSLLVAAEAKLGVKFPR
                     DKIFIRTNLAGAKAALTKWIGTL"
     gene            complement(7964..8662)
                     /locus_tag="VMZ53_12920"
     CDS             complement(7964..8662)
                     /locus_tag="VMZ53_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29411.1"
                     /translation="MRGLVAFCALAGCGRIEFSDVTDARPDAVPPGFCATATFSNPPA
                     SSLHDDFSVDPFTDRWSPVNPMMPCIVQTGGELVATPDSQGLFCHAYTIGDVHLTCDA
                     ITVRVPEVTSPAELGIQTFMYAYVPAVDTSVAVIVEGGGLQIGVIGKTGEQLAGSVFD
                     PTQDRWWRLREHDGELAFDAAVDGIAWRELMRAPTPLSLDHVTIALGAGTYKPVGPPG
                     QARFRCYNVPPPCN"
     gene            complement(8664..9704)
                     /gene="hemH"
                     /locus_tag="VMZ53_12925"
     CDS             complement(8664..9704)
                     /gene="hemH"
                     /locus_tag="VMZ53_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HUS29412.1"
                     /translation="MPTGLLLINLGTPDEPTTPAVRRYLAEFLSDPRVIDINAVGRAV
                     LLHGVILRTRPAKSAEAYQKVWDAKTGSPLLHYSKELAAGVAAKLGETWRVELAMRYG
                     NPSIASALDRLAAAAVDRIVVLPLYPQYAASSTATSVARVMELQTSQWDVTPIDVVPA
                     FHADPGFLAAWEAVARPALAAAKPDHVLFSFHGLPERQIQKSDPSGSHCLKSETCCDA
                     LGPTNHRCYRAQCYATARDLVARLGIASSTVCFQSRLGRTPWITPHTDVLLDELPKQG
                     VKRLAVMCPAFVADCLETLEEIGIRAKEQFKAAGGEELTLIPSLNATPAWTDAVAAMA
                     ARHAARLPVLST"
     gene            9942..10481
                     /locus_tag="VMZ53_12930"
     CDS             9942..10481
                     /locus_tag="VMZ53_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29413.1"
                     /translation="MAGYAGKPLPKKLGIKAGHKVCLLNAPGAFARHLQQHEDVRIAH
                     DLRLPPVDVVVLFVDRVADLERRFADITSRMHPSGGFWVAFPKARRGCDITEDIVRRI
                     ALACGMNANKVCSMGDCLGLRLVLRAEHRDAVAYRAAPPPPISRRVRRPTAAARVVHR
                     RSSSGAGSTLHRARARSTK"
     gene            10548..11102
                     /locus_tag="VMZ53_12935"
     CDS             10548..11102
                     /locus_tag="VMZ53_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29414.1"
                     /translation="MVRLLVASALFAAAASGCVGEEGPDYRPLELGYLTQSVFAPTCG
                     ATQCHSTFKQAATDVFDTPDGVRRSLVNNALVRLDPAKYDPDDAKNADLIIWITQIDP
                     FGAGIGRMPYDAPMPNRDVKLLIEWIEHDAPGAQCNPELNSGKSCTVDSSGRNVVANC
                     SDDWNFDLSTAIPCTSGCAAGQCL"
BASE COUNT         1857 a         3645 c         3671 g         2104 t
ORIGIN      
        1 cgacgtcgat cggcttcgcg gttgcttcga ggttgcgaat cgcttcgaac acgaccttgt
       61 gctggtggtg atagaagtcg tcggtctcga gcgtctctag ctggagcagg atctcgttgc
      121 gcaggatgat gccgccaagg atcgacgctt cggcgtcgag gttgtgcggc agcacgcggc
      181 ggtcacggga catcgtgccg acaatacgat ctcggtacga caatcgaaat cgttaaggcg
      241 tcagacgcgc gcgaagtttc ttctcgagct cgacggggac gagcttctcg cacgcatgct
      301 cgtgcgctag cgcatccgga ttcgactgcg caatgcacgt gcgcagctcg agcggccagt
      361 cgtcgttcaa gcacgaggcg agaatcgtcg gaagcgcatg atcgacgagc atcgtgtagc
      421 tcgcgtcata tgcgacgacc gctgcgcgaa tgcgcggcat cagcgcgaca cacgaggtgg
      481 gcgctggcac cgcctctggc ttcacaccgc gatcgcaagc gaccacgagt gccaagccga
      541 gcagcgcgac tacttgtctt tgccgacgac ccagaacttc agcgtcgcgg tgacacccgc
      601 ggcgagcttg accggcactt cgtacttgcc gagcgccttg accgcgccgt cgagcgcgac
      661 ccagcgatgg tcgatctcga cgttcgcctt cttgagctga tcggcgatgt cgcggctcgt
      721 gacggagccg aacagcttct cgtcttcgcc ggtctggcgc tcgaactgga gcgtcatgcc
      781 gttgatgcga gcggcgatgt ccgtggcgtt cgcgcgctct ttggcgacgc ggcgctcgat
      841 gagagccttc tcgtggtcga ggcggttctt gttcttgacg gtggccgtga cagccatgcc
      901 gcgcggaacg aggtagttgc gaccgtagcc agggcggacc gagacgagct cgccagcctt
      961 gccgaggttc tcgacgtcct gcgtgaggat gatttgcatc gccatgacta gtctcccgtc
     1021 accgagaacg gcagcagcgc cagcatgcgc gcgcggcgga ttgcggtgga gatgacgcgc
     1081 tgctgacgag ccgatgcgcc gctgatgcga cgcgggatca tcttgccgcg gtccgtaacg
     1141 aacgaccgca gcacctgcgg gttcttgtaa tcgaccttct ggaagatctc gtcggagagg
     1201 agcttgcggc gtgttgcacc gcgcgcgcct ttgccaccgc ggcgatcgtc gccgtcgcgg
     1261 tcgagaccat catcacccat gcctgcgtcc atgcgagtcg acatggctta ctccttctcc
     1321 gtggtgggga cgttgcccga gaccggctcg tcggcaaaat catcgctgta atcgtcatcg
     1381 tcgtcgtcgg acgacgagct atcgtccgtg ccgcgcgaca ggaacaagtc ttcttcgtca
     1441 gcggccgtct gcgcggcctt ctcgaacgac gagtcgtcga gctcgctcgg acgagcggcg
     1501 acgtcgatgt cggtgtcgac cttcgtcgtg aggtagcgga tcacgctgtc gagcatgcgg
     1561 aggttgcgct ccgtctcctc gacgacgccc ggctgcgcca ggtactgcca gtagaggtag
     1621 atgcccttgc gctctttggc gacttcgtaa gcaaggcggc gcttgcccca gttgtcgacc
     1681 ttgatgatct tgccgcccat gccctcgatg attcccttga tgcgggtgtt cacctcggcg
     1741 acgccctcgt tcggggtgtt cggacggagg atgtagatcg tctcgtattc gcgcgccgta
     1801 ttcggcgtgt ctctcagtga ctgcagtcgt gccatttcga ttgcctcttg gatgtaaagc
     1861 cctcccttgg cagaggaaga gcgaggttgt ggagggcggg tctatatcac gaacttgcga
     1921 ggttggaacg ggcgttgtgc ttgttcatcg cgggcacgat tccgaggccg agcgtcgctt
     1981 cggtgtcctc gcaacccgcc gagattactc gctcgagagc ggcggcctgc gccgacggaa
     2041 agtctgcgag aacccacgag gccgcgtcct tgctgcccgg tggcttgttc gggtcgcgac
     2101 ccacgcccat gcgcagccgc gcaaacgcgc cgccgccgag ctgctccatg atcgagcgca
     2161 gcccgttgtg gccgccgtgg ccgccgccgt tcttgagccg cacgatgccg aagtcgagat
     2221 cgatctcgtc gtggatgacg aggatcttgt cgggctcgac cgagtagaac tgcgccgcac
     2281 gcgagaccgc gaagccggag aggttcatga attcgagcgg cttgagcagc acggccttct
     2341 cgcgtccgcg ctcggtcgtg acgagcccgg tcgtggcttc gccgcctttg acgctcgcct
     2401 tccatgcggg cagcgcgtgc ttcttcgcca gctcgtcgac gaccatgaag ccgacgttgt
     2461 gccgattgcg cgcgtacttg ctgcccgggt tgccgagtcc gacgacgagc cacatgacga
     2521 tgaccttcta aaaaacgaaa aggcgagcac ggtgctcgcc tcgcagatcg cttctcgaat
     2581 gagagagcga ctacttcttc ttggccggag ccgcggccgc cttggcgtcc ttcgcgccag
     2641 ctgctgccgg agccgccttg gcatccttgg cgcccgctgc gggagccgcg cctgccgccg
     2701 gagccgcacc cgctgccggg gctgcacctg ctgcggcgcc tgctgctgct gcatcgcccg
     2761 ctgccggcgt ctcgaccttc tcctcttccg gcttcgcgca ggtcacgacc gcgaggtcac
     2821 gacccgtcac cgcggtgacg ccggtgggga gagcaatcgc gttgacgtga atgacgtcgc
     2881 cgatttcgag cggcgagaca tcgaccgtca gcttgaccgg gatgtcgctc ggcttcgcgc
     2941 gaacttcgag tgcgcgaatg acgatgcgga tcatgccgcc gtcgacagcg cccttcggct
     3001 tgccaacgaa ctcgagcgga acgttcgcgt tgacttcctt gttcggatcg atcgcgagga
     3061 ggtcgacgtg cgtgatctcg cggcgcagcg gatggagctg gtactccttc acgagcgcgt
     3121 ggagcttctg ggcctgaccg ccgccttcga tcgtcagatc gatcacggtg ttctgcttgc
     3181 gaacgggatc gagcgcggcg cgcagcgcct tcacttcgac gacgatcgac acgggctcga
     3241 tgcggccgtc cggcgacgcg ccgtagcaaa cgcccggaac tttgccctgc tggcgcagct
     3301 tgatcgcagc gcccttaccg gacgaatcgc gcagggcaac ggtgagcttt ccaatttcgg
     3361 gcttctttac ttcggtcgac gtggccatga cagattccct tcgagatcag acgaacaggc
     3421 tggagatcga gtctccatga tggatgcgct tgatcgcttc gccgagcaac cgcccgacgc
     3481 tcttcacgcg caccttggaa cattcttgac ccgcctcgga cagcgggatg gtgttggtaa
     3541 cgacgacgtt ctgcagcggc gactcggtga tgcgaggaac agccttgccc gagaacaccg
     3601 gatgcgtcgc acacgacgag acgctggcag cgcctgcatt catgatcgcg tgcgcggccg
     3661 acgtgagcgt gccggcggtg tcgatgatgt cgtcgacgat gacggccttc ttgcccttca
     3721 cgtcgccgac gatgttcatc acttcgctga cgttcggcgc cggacggcgc ttgtcgatga
     3781 tggcgaggct cgcaccgagg cgcttcgaga aggcgcgtgc gcgctcgacg ccgcctgcat
     3841 cgggagagac gacgacggtg ctgtcgccgc cgaagccgag cacgcgaagc tcgtcgatga
     3901 ggacgggcat tgcgaacaag tgatcgaatg gaatgtcgaa gaagccctgg atctgaccag
     3961 cgtgcagatc catcgcgagc gcgcggtccg caccagcagc cgtgatcaag ttcgcgacga
     4021 gcttgctcgt gatcggcgta cgcggcttcg ctttgcgatc ctgccgcgcg taaccgaagt
     4081 acggcatgat cgcgacgatc gagccggccg acgcgcgctt gagcgcatcg atcatgatga
     4141 gcagctccat cagcgactgg ttgggcggcg agcacgtcgg ctgcacgacg aagcagttca
     4201 cgccgcgcac gttctccccg atctcgacgt agatttcgcc gtcactgaaa tgcgtgacgt
     4261 ccgcgcggcc gagcggaatc tccacgaact tgcagatttc gtctgcgagc gcgcggttcg
     4321 cgttaccagt gaaaatagat agggacttca gcacggtagg gcggaggcgt tggaaccggc
     4381 gatctaccgt tatcagcgca aaaggtcaag agtcgtcttg ggatacgcgg agcgtcccga
     4441 cgagagctaa gcgttcggaa ggcctagctc cttggccttg gcgacgaacc ggtcgacttc
     4501 cggctgcgaa agctcggtcg cctcgtctac gaggagcacc gggatccctc ggtcgatccg
     4561 atacgaaagc ctcgacgcgg ggcacacgag cttctgctcg gcctcgaagt acacgagctt
     4621 ctgcttcgat ttggggcaga cgagcgtctc gaggagctcc ggcgcgaggg gcatgccgct
     4681 caggtagcac ccctcgcgaa ggatgagaaa ggcgcggagt agccgaaaac gaaacgaggc
     4741 cctttcgggc cccgctcgct acgtgcgttc agctccgatt agggaggtgt cggcatcgcg
     4801 ctgccaccct gcgccggtgc gggagccgca ccattgccgg ggaccaggtt gctgatcagc
     4861 gtgaactcga cgcggcggtt cttggcctga gccgccttga gcttgccacc cttgaggccc
     4921 gtcggatcga cggccggctt gtcaggaccg aagccgatcg cggtgaggcg gctctcttcg
     4981 atgcccgcct tcacgaagta cgctttgacc gcgtcggcgc ggccttgcga cagcgacttg
     5041 ttgtcctgga actgcgagcc cgccttcggc ttcaggggca cgtcgtcggt gtgaccttcg
     5101 atctggagct tcaggtcgga gaactccttg agaactgcga cggccttgtc gagcgtcttg
     5161 ttcgacgtcg gcagaagctc ggcggagctc gtcttgaagt tgatgccttg gatcgcgccg
     5221 gtgaactgct tcaccttctg cgggatctcg tcagggcaac cgtcctcgtc ctgatagccg
     5281 ttcttggtct ccggctgatc gatgcacttg tccgcgccgt cgaggacgcc gtcaccgtcg
     5341 ttgtccggat cggggcagcc gtcttcgtct tggaagttgt ctttgtcttc cggctccgtc
     5401 gcgcacttgt cggcggtgtc ggggatgccg tcggcgtcgt tgtccgggtc gggacagccg
     5461 tcttcgtctt ggaaggagtc cttgtcttca ggctccatct tgcacttgtc ggtcgcgtcc
     5521 ggaacgccgt cgccgtcgtc gtcgagatcg gggcagccgt cgtcgtcctg gaagccgtct
     5581 ttgtcttcgg cttcgttcgg gcacttgtcg acgtcgtccg tcaggccgtc gccgtcggtg
     5641 tcgttcgggc ccttcggcgt ctcgacaacc ttctcgacaa ccttgcgacc gaactccttg
     5701 tagatggaga gcagcgcttc gaagtcgagc gtcgggccgt taccgctcga gctcggcggg
     5761 aagagcagac gcgcatccgc gcgaaggccc cagccattgt cgacgcgata cttggcgcca
     5821 acgccgatgt acatcgcggc gtcggtgtct ttctcgacga ggtcttcgtt cttcgagtcg
     5881 atgaccgagg tcgcgccgcc gccaacgagg gcaaacggaa cgagcttctt ggccggatcg
     5941 gctgcgccga actgcgcgat gaggtgcgcg cgatacgtga cgttccagat gtccgcgagg
     6001 gacatgccgc gcgtctcgct cggaatgacg cccacttcgc cttcgacgcc caacatatcg
     6061 ttgaacgtca cgccgaggcg gaggccaaag agtgccgagt tgcgctcgct cggagcgtcg
     6121 tcgacatcgg gcacgccgag ctcgttcgtg gtgctgaaga cgtggatgcc ggcagtgccg
     6181 ccgatctcta cgttggccga cgccgtggac ggcgcgaccg tcgctgcggc cgcaagcccc
     6241 agcgcagcaa tggtgggtct aaacaggcga ttcatggagt ctcccgcagg tgttagcgat
     6301 acgttcttat ccgcgatgga acctgaattc cagcggttgc gccagtttag ttcggggatc
     6361 ttccgatcag gctcccctga cgatgcggta gggtgccccg catgcaggac gctcgcgcgc
     6421 agctccaagc gatgctccgc cccagtgctg gtggtctcta cgtcgtctcc accggtcgcg
     6481 ctgcccagct cgaagtgcag aaacatctct acggcaccga cgacgaatcg cagatccacg
     6541 cgcggttcac cgcggcactc gatcggatcg ccacggcgcg cgccatcatt ctcggggtcc
     6601 catcagacgt cggcgcgggc taccggcgag gcgccaacct agggccgcag gcgattcggt
     6661 ccgctctgat cgctgccgat cccgatctgc gggcgagctt cgacaaactg ggggttgtcg
     6721 acatcggcga tgtcgccgtc gtcccccagc tgctgcacga ctcgatgctc tcgacccagc
     6781 agatcgaagc gagccgcgcc gctctctata aagatgcgcc gcctgcgctc gctgcgcagc
     6841 tgcccgtctc gccgctctcg atcgaagcac gcgtgctcga cctcgtgctc gcgatcaacc
     6901 cgcgcgtcat gccgatcgtg ctcggtggcg atcactccgt cgcgtggccc gtcgccgaag
     6961 cgctcgcgaa gcaccgcacc gatcgctgga gcatcgtcca gcccgacgcc cacaccgacc
     7021 tcctctccga gcgcctcggc atcaagtact gctacgcgac gtggagctat cacgcgaacg
     7081 agctcctcgg ccgcgatggc aggctcgtcc aagtcggcgt gcgcgcgtcg cggtttcagc
     7141 gcgagcactg ggaatcgacg ctcggcgtgc gccaattctg ggcgagcgag atcgaggccg
     7201 atcgcgagcg cgcactcgac gacgtcatcg cgcatctcaa ggcgcgcaac gtcaccggcg
     7261 tgtacttctc gaacgacatc gacggcaccg acgcgaagtt cgccgaagcg acaggcacgc
     7321 ccgaaccgga cggcctcgat cccgattggc tgctcgcgct gatcgcgcgc ctcggcaaag
     7381 agatcgggct catcgccgcc gattgcgtcg aggtcgcgcc tccgctcggc ccgacgccgc
     7441 aggccaccga cgccacgacg cgcctcgctg ctcgctacgt ccgcgcctgc ctcgatcagc
     7501 tgcgctgacg cagatcggta cgcgctagag cgtcccgatc catttcgtga gtgctgcttt
     7561 cgcaccggcg aggttggtgc gaatgaagat cttgtcgcgt gggaacttca cgccgagctt
     7621 ggcttcggcc gcaacgagca agctgccgag ctccgacagt ggcggcgggc tcttgccctt
     7681 gacgaccttc agctggtcgt cgagctcgac ggtgtcgtgc acgcgaatgg aggtcgctgc
     7741 accgggtttg ctgacgagaa ggttccattc gtcggcgaag acagtgaggc gaaagacggt
     7801 gtccacggtg ctcgtgtaga cgttgctggc tccgccggtc gcgtcgagga acacttccgc
     7861 gcgccacttc ggcttgctcg ctgccacctt cagcgacttc aaccagtctg cggcttctcg
     7921 cgctcctcgc gatttctcac tcggcatcgc ggtggttatc gcatcagttg cagggcggcg
     7981 gcacgttgta gcaacggaag cgcgcttgcc ccggtgggcc gacgggcttg tacgttcccg
     8041 caccgagcgc gatcgtgacg tgatcgagcg acagcggcgt cggtgcgcgc atcagctcgc
     8101 gccacgcgat tccgtcgaca gccgcatcga acgcgagctc gccgtcgtgc tcgcgaagcc
     8161 gccaccatcg atcctgcgtc ggatcgaaca ccgagcccgc aagctgctcg ccggtcttgc
     8221 cgatcacgcc gatctggagg ccgccgcctt cgacgatcac cgcgacgctc gtatcgacag
     8281 ccggcacgta cgcatacatg aatgtttgaa tgccgagctc ggccggcgag gtcacctcgg
     8341 gaacgcgcac cgtgatcgcg tcgcacgtga gatggacgtc gccgatcgtg tacgcgtggc
     8401 agaagaggcc ttgcgaatcg ggtgtagcga cgagctcgcc gcccgtttgc acgatgcacg
     8461 gcatcatcgg attgaccggc gaccagcgat cggtgaacgg atcgacgctg aagtcgtcgt
     8521 gcaggctgct cgcgggtgga ttggagaacg tcgcggtggc gcagaatccg ggcggcaccg
     8581 catcggggcg cgcgtccgtg acgtcggaaa actcgatacg cccgcatcca gccagcgcac
     8641 agaacgcgac gagcccccgc accctacgta gagagtaccg ggagacgagc agcgtggcgc
     8701 gcagccatcg ccgcgactgc atcggtccac gcgggggtcg cgttgagcga cggaatcagc
     8761 gtgagctcct cgccgcccgc ggccttgaac tgttccttcg cgcgaatccc gatctcttcg
     8821 agcgtctcga ggcaatcggc gacgaacgcc gggcacatca cggccagccg cttgacgcct
     8881 tgcttcggca gctcgtcgag gaggacgtcg gtgtgtggcg tgatccacgg cgtgcgcccg
     8941 agccgcgact gaaagcagac ggtggagctc gcgatgccca gccgcgcgac gaggtcacgc
     9001 gccgtcgcgt agcactgcgc gcgatagcag cgatggttcg tcgggccgag cgcgtcgcag
     9061 cacgtctccg acttcaggca gtgactgccg gatggatcgc tcttctggat ctggcgctcg
     9121 ggcaggccgt gaaacgagaa cagcacgtga tcgggcttgg ctgccgcgag ggcagggcgc
     9181 gcgaccgctt cccacgccgc gagaaatccc gggtcggcgt ggaacgcggg caccacatcg
     9241 atcggggtga catcccattg cgaggtctgc agctccatca cgcgcgcgac cgacgtcgcc
     9301 gtcgacgatg ccgcgtactg cggatagagc ggtagcacca caatccgatc gacggccgct
     9361 gcggcgagac gatcgagtgc actggcgatc gacgggttgc cgtaccgcat cgcgagctcg
     9421 acgcgccatg tctcgccgag cttcgcagcg acaccggccg cgagctcctt cgaataatga
     9481 agcagcggcg agccggtctt ggcgtcccac accttctggt acgcctcggc gctcttcgcc
     9541 ggtcgcgtcc gcaggatcac gccgtgtagc aaaaccgcac gaccgactgc gttgatgtcg
     9601 atgacgcgcg ggtcggagag gaactcggcg aggtagcgac gaaccgccgg cgtggttggt
     9661 tcatcgggcg ttcccagatt gatgagcagg agaccagtgg gcacagacgt cacaccttgc
     9721 tgaccaagtg ggaccgcaaa gtcaaggaaa cggccggccg ggagcgagac atttcatagt
     9781 cgcccagaaa tccacgctat ccacaggcgg aatggtcatc gtccctacga aagagcggga
     9841 gtacggtgat cgcaccaaag tctagggtct gccgagactt agaagaagat ttggccatcc
     9901 tgtttgacct gccgcggggc ggctgcatta tccaacacgt catggctggc tacgcgggaa
     9961 agccactacc gaagaagctt gggatcaagg caggacacaa ggtgtgtctg ctgaacgccc
    10021 cgggcgcatt cgcacgtcac ctccagcagc acgaggacgt tcgcatcgcg cacgaccttc
    10081 ggttgccgcc ggtcgatgtg gtcgtgctct tcgtcgaccg cgttgcggat ctcgagcgcc
    10141 gctttgccga catcacctca cgcatgcatc cctcgggcgg cttctgggtc gcgttcccga
    10201 aggcgcgtcg cggctgtgac atcaccgaag atatcgttcg tcgaatcgcg ctcgcctgcg
    10261 gcatgaacgc caacaaggtg tgctcgatgg gcgactgcct gggccttcgc ctcgttctgc
    10321 gcgccgagca tcgtgacgcc gtcgcgtatc gcgccgcgcc gccgccgccg atctctcgcc
    10381 gtgttcgccg tccgacggcc gctgcgcgag tcgtgcatcg ccgctcgtcg agtggtgccg
    10441 gctccacgct gcatcgcgct cgcgctcgtt cgacgaagta agtcgcgaga cattctggtg
    10501 caccgagcga gtgcccggaa actctctcat tgtagtaggg tccgcgcatg gttcgactcc
    10561 tggtcgccag cgcgctcttc gcagctgcag cttccggatg cgtgggcgaa gaggggccgg
    10621 actaccggcc tctcgagctc gggtacctga cgcaatcagt cttcgcgccg acgtgcggag
    10681 ctacgcagtg tcactcgacg ttcaagcaag ccgccaccga cgtgttcgat acgcccgacg
    10741 gcgttcgccg ctcgctggtg aacaacgcgc tcgtgcggct cgatcccgcg aagtacgacc
    10801 ccgacgacgc gaagaacgct gacctcatca tctggatcac ccagatcgat ccgttcggcg
    10861 ctggaatcgg gcgcatgccg tacgacgcgc cgatgcccaa ccgcgacgtg aagctcctca
    10921 ttgaatggat cgagcacgac gcgccgggcg cgcagtgcaa tccggagctc aacagcggca
    10981 agtcgtgcac ggtcgattcg tcgggacgca atgtcgtcgc gaactgcagt gacgattgga
    11041 acttcgatct gtcgaccgcg attccgtgca cgagcggctg cgcggcgggg caatgtctat
    11101 gacccgcgtc gtcctcgccc ttctgatcgt cgccgcggct gcgggttccg cgcacgcata
    11161 tccgcagttc gagctcgcga tggatcagtc gtgtgccggc tgtcacatct cgccgagtgg
    11221 cggcggtccg ctctccgaga acggcatggc cgtcgccgag tcgatctcga agttcgg
//
LOCUS       DATLBZ010000244         5863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4726650, whole genome shotgun sequence.
ACCESSION   DATLBZ010000244 DATLBZ010000000
VERSION     DATLBZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4726650"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VMZ53_12940"
     CDS             <1..638
                     /locus_tag="VMZ53_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29415.1"
                     /translation="FEENHDLTDGGRSVTGWGAAPYIGITDQLELQLPVEFEWFGKTG
                     AGAGTAFTTYGGELRYRMVTNDAENRPPFAPLVRVGAHRIIRTRDVAELNVGLSGSYE
                     SGIVHALADVNFIGDVDFNDRDTDPDDGSEFAIRAGAGVSVEVYDDIRLGAELFAHVG
                     LGDAAMGEEKMWIIAGPGGSWTHGRFWLSAMYGIGVANIGTAPRVQWGIAF"
     gene            620..1282
                     /locus_tag="VMZ53_12945"
     CDS             620..1282
                     /locus_tag="VMZ53_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HUS29416.1"
                     /translation="MGHRLLAIAVVALVGGNAHADGGRVVGKVTVTEADGKAATADVI
                     VYIVGYTEPGVGKATAQVAQKGRKFVPDLVAVTAGESVSFPNQDSFLHNVFSQSSARK
                     FDLGSFKKGDTKEKDFTTAGVIDVYCNIHPEMAATILVLPNKRHTRTKPDGTFVIDGV
                     KPGKWTVFAYGRRALKPASGTVTVTAGQDATVNLALTRGAEPPHVNKYGEKYKDPTTT
                     YR"
     gene            complement(1273..2160)
                     /locus_tag="VMZ53_12950"
     CDS             complement(1273..2160)
                     /locus_tag="VMZ53_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29417.1"
                     /translation="MPPDRVEQLAKTSGLPASSRTAVVAAWESLLATAREKWPTVTFD
                     EDALVDFIGPRLSGDDLAVALASAPAADLALAAACTAQDKTAHSAFDAVLTEVDAAGI
                     STNATKDVVTEVKQVLRMQLLVPKDSKPPGIVGYRGKGPLRGWVRITATRELIRHKKK
                     RQREAPLSDRTLDHLLTSGIDPGLAALKAEYRTEFALALREAIEDLGVEDRTLLRQQI
                     VDGLSIDAIGAAFGVHRATAARWLNRARGALVAATHRRLAARLKLPVEQIESVIRLVS
                     SQLDASVIRYLRDGSSVQR"
     gene            2193..4268
                     /locus_tag="VMZ53_12955"
     CDS             2193..4268
                     /locus_tag="VMZ53_12955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29418.1"
                     /translation="MECLDANVVQDLMSGALDSQVRLQVLGHIDTCDDCRELLNITAS
                     DTTNESLPQFHDSMNIGLEATQAAGDVGLEETKPANPHSLAGARIPRSPEEGKRLAHY
                     TLIERLGAGAMGVVWKADDPKLGRQVAVKVLKRADEALTERLVREARSMAQVNDPHVV
                     TVYEVGEGDGTTFIAMELVSGKSLRAWQRERTVPELVNAYSAAGRGLAAAHAAGIVHR
                     DFKPDNVLVGDDGRIRVTDFGLAAAKPTPGAASPQIGDVNLTTSGSVLGTPAYMAPEQ
                     FTGGNVDPRTDQFNFCVALYEALYGERPFEGKTFAELGDNVMHGQIKPAPSGSQVSSA
                     LRAILLKGLSPKPGDRFPTMNDLIKELGRDRARPWRRTAVIAGALAIALLIFLAADWV
                     VRARVSGEIHQSFALTKSQTDKTMSRLTESFSRSSEVAYQVSALRELAGHHDQFDFGL
                     GTADADTAELERLHGTFEAADWGMLHGTQLIIGDYKGRLIYTSAAPKEWGGDLRVFES
                     VKRALDAGKFDSIEILSYADSRVAASGILGKAAPKQGIGLLMEKTLALGETEGREARA
                     LYLQLQDGKKLLEEIQLDDETGLALVTPDGTSLSNAGMRPELIAAATSGADEARIDGK
                     TYQLQSFPVLGLDKQTPIGRVVMARPNDAVLSIFPGARLVFALTMFAALLLAAFTWNR
                     ARQITVGRV"
     gene            4371..5342
                     /locus_tag="VMZ53_12960"
     CDS             4371..5342
                     /locus_tag="VMZ53_12960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29419.1"
                     /translation="MRAFAPGDVFADRYEILASAGHGGMGHVYRARHITLRKQVALKV
                     LASNPTGDYEIRFAREARALARLDHPNCVRVLDHGQHDATQFLVMEHLEGETLTARIA
                     QGRLPTSNALQIARQLLFALAHAHEHGVIHRDVKPENVMLVKGGTRAILIDFGLASIA
                     DEAALTGAGMCLGSPSYLAPERLLGQPHDTRTDLYAVGVILYEMVAGVRPFAGDSPRE
                     TMQLAIHRPPRPLRAVRRDIAPALERIISRALAKDPARRFADAEEMLLALADVPSDED
                     AAARKVQDERDEAAETLMISTLDVKEPGVLSRAWTWVRYGGWRWRHS"
     gene            5359..>5863
                     /locus_tag="VMZ53_12965"
     CDS             5359..>5863
                     /locus_tag="VMZ53_12965"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HUS29420.1"
                     /translation="MSALVLLAATASLAPARVEHAPTIDGHLDDAAWTSIPTTTAFTQ
                     AFPDDGKPPSDPTSVRVAYDDENLYVAIACEQREPIVARLTRRDREIEGDRVSIDLDT
                     SGDKRGAFHFQVSAAGVMVDALRYDDTEVATEWDEIWRAEIATTPTGWSAELAIPLRI
                     LRLRRGVA"
BASE COUNT         1015 a         1925 c         1965 g          958 t
ORIGIN      
        1 cgttcgagga gaaccacgac ctgaccgacg gcgggcgctc ggtgacgggg tggggcgcgg
       61 cgccgtacat cgggattacc gatcagctcg agctccagct gcccgtcgaa ttcgagtggt
      121 tcggcaagac cggcgcgggc gcgggcactg cgttcacgac gtacggcggt gagctgcggt
      181 atcggatggt gacgaacgac gccgagaatc ggccgccgtt tgcgccgctc gttcgcgtcg
      241 gcgcgcatcg catcattcgc acgcgcgatg tggccgagct caatgtaggg ctcagcggct
      301 cgtacgagtc cggtatcgtg cacgcgctcg ccgacgtgaa cttcatcggc gatgtcgact
      361 tcaacgacag ggacacggat ccggacgacg gctcggagtt tgcgattcgc gcgggcgcgg
      421 gcgtgagcgt cgaggtctac gacgacattc gcctcggcgc ggagctgttc gcgcacgtcg
      481 ggctcggcga cgcagcgatg ggcgaagaga agatgtggat catcgcgggg cccggtggct
      541 cgtggacgca cggccgcttc tggctctctg cgatgtatgg gatcggagtt gcgaatatcg
      601 gcacggcgcc gagggtgcag tggggcatcg ccttctagcg atcgccgtcg tcgcgctcgt
      661 gggcgggaac gcgcatgccg atggtggccg cgtcgtcggg aaagtgacgg tcaccgaggc
      721 cgacggcaaa gcggcaactg cggacgtgat cgtctacatc gtcggttaca cggagccggg
      781 cgttggcaag gcgaccgcgc aggtagcgca gaagggccgc aagttcgtgc ccgatctcgt
      841 cgccgtgacc gccggtgaga gcgtgagctt tccgaatcaa gattcgtttc tgcacaacgt
      901 gttctcgcaa tcgagcgcgc gcaagttcga cctcggctcg ttcaagaagg gcgatacgaa
      961 ggagaaagac ttcacgacgg cgggcgtgat cgacgtctac tgcaacatcc atcccgagat
     1021 ggccgcgacg atcctcgtct tgccgaacaa gcgccacaca cgcacgaagc ccgacggcac
     1081 attcgtcatc gacggcgtga agccgggcaa gtggaccgtg ttcgcgtacg ggcgtcgcgc
     1141 gctcaagccg gcgagcggga cggtgaccgt gaccgccggg caagacgcga cggtgaacct
     1201 cgcgctcacg cgtggcgccg agccgccgca cgtgaacaag tacggcgaga agtacaaaga
     1261 tccgacgacg acttatcgct gaacgctgct gccgtcgcgc aggtagcgaa tcaccgatgc
     1321 atcgagctgg ctcgacacga gccggatgac gctctcgatc tgctcgacgg gcagcttcaa
     1381 ccgggctgcg agccggcgat gtgttgccgc gacgagcgca ccacgcgcgc gattgagcca
     1441 gcgggccgcg gtcgcgcgat gcacgccgaa ggcggcgccg atcgcatcga tcgagagacc
     1501 gtcgacgatc tgctggcgca gcagcgtgcg atcctcgacg ccgagatcct cgatcgcttc
     1561 gcgcagcgcg agcgcgaact cggtgcggta ctcggctttg agtgccgcga ggcccggatc
     1621 gatcccgctc gtgagcaagt ggtcgagcgt gcggtcggac agcggtgctt cgcgttggcg
     1681 cttcttcttg tgacggatga gctcgcgcgt cgcggtgatg cgcacccagc cgcgcagcgg
     1741 gcctttgccg cgatagccga cgatgccggg aggcttgctg tctttgggca cgagcagctg
     1801 catgcgcagc acttgtttca cttcggtgac gacatccttc gtcgcgttcg tcgagattcc
     1861 ggcggcgtcg acttcggtga gcaccgcatc gaacgcgctg tgcgcggtct tgtcttgcgc
     1921 ggtgcatgcg gccgcaagag ctagatccgc ggcgggcgcg gatgcgagtg caacggcgag
     1981 gtcgtcgccg gagagtcgcg ggccgatgaa gtcgacgagc gcgtcttcgt cgaacgtgac
     2041 ggtcggccac ttctcgcgcg cggtcgcgag cagcgattcc cacgcagcga cgaccgcggt
     2101 tctcgacgac gcgggaagtc cgctcgtctt cgccagctgt tcgacgcggt cgggcggcac
     2161 gccttgattg tctcatgtca caatggctcc gcgtggagtg cctagacgcc aacgtcgtcc
     2221 aggatctgat gtcgggcgcg ctcgacagtc aggtgcgcct ccaagtgctc ggccacatcg
     2281 acacctgcga cgactgccgc gagcttctca acatcaccgc gagcgacacg acgaacgagt
     2341 cgctgccgca gtttcacgac tcgatgaata tcggcctcga agcgacgcaa gcggcgggcg
     2401 acgtcgggct cgaggagacg aagccagcca atccgcacag cctcgccggt gctcgcatcc
     2461 cgcgctcccc cgaggagggc aagcgactcg cgcactacac gctgatcgag agactcggcg
     2521 caggtgcgat gggcgtcgtg tggaaggccg acgatccaaa gctcggtcgc caagtcgcgg
     2581 tcaaagtcct caagcgcgcg gacgaagcgc tgaccgagcg gctcgtgcgt gaagcgcgat
     2641 cgatggcgca ggtcaacgac ccacacgtcg tcacggtcta cgaagtcggc gagggcgacg
     2701 gcacgacgtt catcgcgatg gagctcgtgt cgggcaagag cctgcgcgcg tggcagcgcg
     2761 agcgcaccgt gcccgagctc gtcaacgcgt acagtgccgc agggcgcggg ctcgctgcag
     2821 cgcatgccgc gggcatcgtt catcgcgact tcaaaccaga caacgtcctc gtcggtgatg
     2881 atggccgcat ccgcgtgacg gacttcggtc tcgccgccgc gaagccaacg ccaggcgctg
     2941 catcgccgca gatcggcgac gtgaacttga cgacgtcggg ctccgtgctc ggcacgccgg
     3001 catacatggc gccggagcag ttcaccggcg gcaacgtcga tccgcgtacg gatcagttta
     3061 acttctgcgt cgcgctgtac gaagcgctct acggcgagcg accgtttgaa ggcaagacct
     3121 tcgcggagct cggcgacaac gtcatgcacg gtcagatcaa gccggcgccg tcgggctcgc
     3181 aggtctcgag tgcgttgcgc gcgattctcc tcaaggggct gtcgccaaaa ccaggcgatc
     3241 gctttccgac gatgaacgat ctcatcaagg agctcggtcg cgatcgtgca cggccgtggc
     3301 gtcgcaccgc ggtcatcgcc ggcgcgctcg caatcgcgtt gttgatcttc ctcgccgcgg
     3361 actgggtcgt gcgcgcgcgc gtgagcggcg agattcacca atcattcgcg ctgaccaaga
     3421 gccagaccga caagacgatg tcgcgcctga ccgagtcgtt ctcgcgctcg tcggaggtcg
     3481 cgtaccaagt gtccgcgctc cgcgagctcg caggtcatca cgaccagttc gacttcggcc
     3541 tcggcaccgc cgacgcggac accgccgagc tcgagcgcct gcacggcaca ttcgaagcag
     3601 ccgattgggg catgctgcac ggcacgcagc tcatcatcgg cgactacaag ggccgcctga
     3661 tctacacgag cgcagcgccg aaagaatggg gcggcgacct gcgcgtcttc gagtcggtca
     3721 agcgcgcgct cgacgcaggc aagttcgaca gcatcgagat cctgtcgtac gcagactcgc
     3781 gcgtcgccgc gtccggcatc ctcggcaagg ccgcaccgaa gcaaggcatc ggcctgctga
     3841 tggagaagac gctcgcgctc ggcgagacgg aagggcggga ggcgcgcgcg ctctacctcc
     3901 agctgcaaga tggcaagaag ctcctcgagg agatccagct cgacgacgaa actgggctcg
     3961 cgctcgtgac gcccgatggc acgtcgctct cgaacgccgg catgcggccc gagctgatcg
     4021 ctgcggcgac gagcggcgcg gacgaagcgc gcatcgacgg caagacgtac cagctgcagt
     4081 cattccccgt gctcgggctc gataagcaga caccgatcgg ccgcgttgtc atggctcgcc
     4141 caaacgacgc cgtgctctcg atattcccag gcgcacgact cgtattcgcg ctcacgatgt
     4201 tcgcagcact tttgctcgct gcattcacgt ggaaccgcgc tcgccagatc accgtcggtc
     4261 gcgtgtgacc gtgcgttcac gctagccacg acatccgtcg acgattgcgt gacggattgt
     4321 ttcgagctct ggcgtgaaca caaacgcgtg tcattcgtct cattatcaat atgcgcgcgt
     4381 tcgcaccggg tgatgtgttc gccgaccgct acgaaatcct cgcgtccgcg gggcacggcg
     4441 gtatgggcca cgtctaccgc gcgcggcaca tcacgctgcg caagcaggtc gcgctcaagg
     4501 tgctcgcgtc gaatccgacc ggcgattacg agatccgctt cgcccgcgaa gctcgggcgc
     4561 tcgcgcggct cgatcatccg aactgcgtgc gcgtgctcga tcacggccaa cacgatgcga
     4621 cgcagttcct cgtgatggag catctcgaag gcgagacgct caccgcgcgg atcgcgcaag
     4681 gccgcctccc gacgagcaac gctctgcaga tcgcgcgtca actcctgttc gcgctcgcgc
     4741 acgcccacga gcacggcgtg atccaccgcg acgtcaaacc cgaaaacgtc atgctcgtca
     4801 aaggcggcac gcgcgcgatc ctgatcgact tcggactcgc gagcatcgcc gacgaagctg
     4861 cgctcaccgg cgcgggcatg tgcctcggct cgccgtccta cctcgcgccc gagcgcctgc
     4921 tcggccagcc gcacgatacg cgtacggacc tctacgccgt cggcgtgatc ctctacgaga
     4981 tggtcgccgg cgttcgtcca ttcgccggtg actcgccgcg cgagacgatg cagctcgcga
     5041 ttcatcgccc gccgcggccg cttcgcgccg tccgtcgcga catcgcgcct gcgctcgaac
     5101 ggatcatctc gcgcgcgctc gcgaaagatc cggcgcgccg ctttgccgat gccgaagaga
     5161 tgctactcgc gctcgccgat gtcccgtccg acgaggatgc ggcggcccgc aaggtgcaag
     5221 acgagcgcga cgaagccgct gagacgctca tgatctcgac gctcgacgtg aaagaacctg
     5281 gcgtcctctc ccgcgcgtgg acatgggtgc gctacggcgg ctggcgctgg cgccactcct
     5341 gataatgtcg cgcggttcat gtccgcgctc gttttgctcg ctgccaccgc cagcctggcg
     5401 cccgcgcgcg tcgagcatgc accgacgatc gatggccatc tcgacgatgc ggcgtggacg
     5461 tcgattccca ctacgacggc gttcacgcag gcgtttcccg acgacggcaa gccaccgagc
     5521 gatccgacga gcgttcgcgt tgcctacgat gacgagaatc tgtacgtcgc aattgcgtgc
     5581 gagcagcgcg agccgatcgt cgcgcggctg acgcgtcgcg atcgcgagat cgagggggat
     5641 cgcgtcagca tcgatctcga tacgagcggg gacaagcgcg gcgcctttca ttttcaggtc
     5701 agcgccgcgg gcgtgatggt cgatgcactt cgctacgacg acaccgaggt cgcgacggag
     5761 tgggacgaga tctggcgcgc agaaatcgcg acgactccca cgggatggtc cgcggagctc
     5821 gcgatcccgc tgcggattct gcgcttgcgg cgaggcgtcg cga
//
LOCUS       DATLBZ010000245        24011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2497379, whole genome shotgun sequence.
ACCESSION   DATLBZ010000245 DATLBZ010000000
VERSION     DATLBZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 24011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24011
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497379"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..528)
                     /locus_tag="VMZ53_12970"
     CDS             complement(88..528)
                     /locus_tag="VMZ53_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29421.1"
                     /translation="MLDGLFVTPAVTAIVEALSSANAEGKLAVIGNAKLATALAETKR
                     EVLPIAMPARASKKLTNALADLSSIDDGSLAGVIGVDIATDDGWELTLKEWRRVVRDG
                     GALVFVDRGHAPEASRRALCSGLSEIEQRHVGRTIVTSGLVSHL"
     gene            complement(542..1300)
                     /locus_tag="VMZ53_12975"
     CDS             complement(542..1300)
                     /locus_tag="VMZ53_12975"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HUS29422.1"
                     /translation="MRFAATLVALAVLAPTARADDSKLAKEAVPLQPKAPLRGKNKLC
                     CGYPLVDDQRWALRFYWMVLERDYNEPASPNAPKSGRGEADRVSEDARVDLYTPKGWF
                     LGNVTEKLAWAVRLEGSLLMEDGRVISYAGKCDYGYGTCFEQLNVKEHPFGRGAKKRP
                     LTPFKSVAVDQRLIPIGEPLYIPEFDGLTLPDGSIHDGCVRADDTGGGIKRRKMDFFV
                     VTYGNFRYLLDQVLGVNWITPHIEAPRCEYLRDL"
     gene            1315..3345
                     /locus_tag="VMZ53_12980"
     CDS             1315..3345
                     /locus_tag="VMZ53_12980"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HUS29423.1"
                     /translation="MIRVWVHDLRDAHDARCGGKARGLATLIGAGVDVPPGFVIDDRA
                     FRNIVGELKIDDVGDIGHALAQAAERIASARIPDELMAAVNDRVGELGHLLAVRSSAT
                     IEDQAAGAAAGVFSSRGSVHVDELWDAIRAVWTSALTPLAAAYARRRGGELSIGVIVQ
                     EFVIGVPTVVYTRPPGQPESHEILVQRNDHVSRHSRADLPREIEHQHAAVLAMRVEQI
                     LAMPSGVDVELVQIRKQSGFDVAMETVVVQARPIVHPKPNALTPPPPNVLAALDDGRT
                     WRWDVAHNPDPLSPAQQGLVERVERTGIAPWSVRVVAGFLYSASRGEPAPSVGEPAAF
                     AARAAELEARLEKTLHVEGTPTVAEAIERYLAFYALWARELSPLIAAAKAAAPASAFV
                     VGTRPSSVEATLLAAARGDISEEDVERQLGCLAPAWDVAVRTYAERPGVLRDAVARAR
                     VALTKHADIVPLPVEQTPEMKLARLAGELAERDDALFARAQLLVRKALLARGKEAGVG
                     EDIFWLPLDEAAATKALDPIDARRRAAGARAAAERAARWQMPLVHPTTEQPATEQSSA
                     IQLRGVGTGPRVGGRVVRFASLASAIAVGAGDVVVTRAVTPALAVLVAGCAALVSESG
                     GLLDHGAALARELGIPCVVGCRNAWSQLADGMLVTVDGEGGTVTTTYSSSSS"
     gene            complement(3324..3689)
                     /locus_tag="VMZ53_12985"
     CDS             complement(3324..3689)
                     /locus_tag="VMZ53_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HUS29424.1"
                     /translation="MTKSELIEKISETLKLPAGKAEAIVNTIFDSMVKALERGEGIEI
                     RGFGSFTVRKYKAYEGRNPRTGETVHVAEKRLPFFKVGKDLRERVNAGLGTPLPADKP
                     DEPDDTDPNEPDDYDDDDE"
     gene            complement(3772..4611)
                     /locus_tag="VMZ53_12990"
     CDS             complement(3772..4611)
                     /locus_tag="VMZ53_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29425.1"
                     /translation="MSDSQFRLGTLAGAFFLVALIVYLRFCGSISLPSKPPPPTGPSG
                     TQRELLNKSTGSKSVYKSFLDSDAQNAGVSSPSVSDMSKKLAYHVEDARHVLEPGKPP
                     IDLAGLRLHLERSSDQVVLVIENKLDSDVAYNVTTSVSSGSSQCTKAPALPFNAMVIS
                     KGSSERRTECLWRDGMSITVTKVETLEVLPLSAWYLSQVSPQQVGVEDRIARGHHGVQ
                     TSETCSQIMPAVVRTGLERGDLGWRDLVDFYARHRCQSYQFPVTYRALKSDGERAIPA
                     VDG"
     gene            4713..5621
                     /locus_tag="VMZ53_12995"
     CDS             4713..5621
                     /locus_tag="VMZ53_12995"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS29426.1"
                     /translation="MFGRGNRIAKLVAVAVLALAVPHRAHAADRVTELTSMLSSSSEK
                     TRISAVLSLARLGDKSALKPLVTALHDPSAQVRAIAATALGKLGHKAALPALKSAATD
                     DVDETVRQKAAEAAASVAKLNHLDESPAEKDASAQARVQQKTSGFGRSPHAVTDRPDL
                     FITVKGANDDSPGTTDKATRKLNADLLKASLQTSLAKAPQVTMAPDEAGRWGLDVRSV
                     DISVVKLELSQDGNYMVMAAELRLAISDDKGKMLSFLSGGAKVQIPRSKYSAKILPNM
                     RKDALEGAMQGMFDKLLVHLRQITTT"
     gene            5639..8254
                     /locus_tag="VMZ53_13000"
     CDS             5639..8254
                     /locus_tag="VMZ53_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS29427.1"
                     /translation="MRVVLVLLIALCAVAHADKVITKQPKLLQAVAPEYPPKALEDGK
                     QAKVKVRIHIDATGIVTKVDVLEPVGDGFDQAAIDAAMQYVFEPAEIDGKPGEIAVET
                     SINFVIEQRPEEEPPPLPTPPQEREGPPNHAGAMSQPITLQGEAIERGTRRRLPGVIV
                     SIAELKLDAVTGEDGTFYFHGVAPGHYQLLAVDPKFDKFERPVVIEKREQLEVRLWMR
                     PRGGNPYETIVEGEREQLEVTKRTLHRQQLTSVPGTFGDPIRVIQTLPGMQRAPFGLG
                     LLLVRGSNPDDTGIFVDGHEVPALFHFLGGPSIFNAEMLESIDLYPGGFPSRFGRHHG
                     GVVAIETRPTKSDGIHGSAKVDLIDSGGYIRAPITKNVSAAIAGRRSYIDFILPLFLP
                     EPSAGSQRIVTPVYYDYQARLDWNLHENGRLSTFLIGSSDKLHVFQKDADTMTSANLD
                     TAVKFFRVMATYERRLGKDLKLTLSPSWGQDSLTFSGAQAENTGPFTSLTLTNKALSY
                     RFRVHGKLRPSLTIDTGIDLLSRATKFDALVPVDDTLIDSSGVDVEPSKLLRGTSTIG
                     IATYADVAIDATSKLKLIPGLRLDGYFINGQERSSVDPRLVVRYKTAPQWTVKGYVGK
                     FTQPPQPESLDSRFGNPKVGLEHGYHYGLGYEWKPERLWSIDSELYYVDRRDVVVFSP
                     DVIDNGDGTFTYENFTNNGRRASYGFEAIIKREISERAFGWLSYTYSHSRQTNRRGEN
                     WFPTGFDQPHVLNAVASFKPGGGWELGTRFQLASGRPDAVVIGSTFDADCGCYDAVVT
                     GNRATRVPTYMQLDARIEKTWLFELWSIGAYLDVINVTNRKNQEGIDYDYRYRQSSPI
                     TSFPIFPTIGVRGQW"
     gene            8251..9222
                     /locus_tag="VMZ53_13005"
     CDS             8251..9222
                     /locus_tag="VMZ53_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29428.1"
                     /translation="MKRAVLLLALSACTTFEDPDIVLDFRVLGAQAEIPEQIIDIDIT
                     MPPKADDVLAQLVDTRVCWLMSDKNFDRSLRWSATLCNLNGDERCPEGSPQANLGSGV
                     WDDPDTAPTPDSFCTNVPANGNLLGVALDYLENDQLHGLGGIYYGVSLRVGGVDGDPA
                     LDLYAAKSLRIQPRIPPELTANTNPTLVQIEASVDGAANVLLVDARCVDQPAPLEVAA
                     GATLRFYPVEPDGVRETYVVPTIDGMVRTFTESLTYQWLATAGNYAGGRSGGTRDAFG
                     NPATLWTDWNAPDAEDLEGPLDVDFWIIQRDERLGLKWFRGCVRVVP"
     gene            complement(9219..9647)
                     /locus_tag="VMZ53_13010"
     CDS             complement(9219..9647)
                     /locus_tag="VMZ53_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HUS29429.1"
                     /translation="MATRMNIDTRAELQQTVRAKGLRATPSRLAVLELLRVSDVPMSH
                     GDVADRLSASSWDRATIYRNLTDLAEVGLVRRTDIGDHIWRFEAINGDHEHPHFVCTE
                     CGTVECLPEIELAVRRAKAPRAIKQRKVEVHVRGLCDACI"
     gene            9686..10957
                     /locus_tag="VMZ53_13015"
     CDS             9686..10957
                     /locus_tag="VMZ53_13015"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUS29430.1"
                     /translation="MSLRSHFETLVRTEPPEVVADRGDEVDPHSVGLDRKIIDAIWQA
                     TMATYRTGLYPALSLCIRRRGEVVLDRSIGHVSGNAPHDPPDSRKVIASPKTLFNMFS
                     ASKMVTAMLVHLCDERGLLHLDDPVARYLPDFGEHGKHRITIRHVLTHRAGIPHIPPQ
                     FADVGLLERPDEILSLLSRQKPRWRAGQRLAYHALTGGFVLGAIVEKVMGKPLRQVMH
                     DEMLGPLGFDAFNFGVPQARLHEVAMNAFTGPPILPPISQLLKRMLSVHFVEAVRVSN
                     DPRFLTSVVPAGNIVTTANEASRFMQLLLNGGELDGHRVFETRTIQRAVAEQTYFEID
                     LTMGAPIRYSMGFILGARFASPYGLNTSKAFGHLGFTNVFVYADPARDIAVGFMTSGK
                     PALSIGITRTLLTMQTIARRIPRDGRGPLRR"
     gene            complement(10946..11842)
                     /locus_tag="VMZ53_13020"
     CDS             complement(10946..11842)
                     /locus_tag="VMZ53_13020"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HUS29431.1"
                     /translation="MRAALAATLLLTAACGADDSMMTTPDAPTGSSDGSLSDGAGGCM
                     RPALAQTWLPTLLNDVITNIPAPRATGTQRTAARNYLQTQLTQMGWSPSLAAYSTGAN
                     VVARIPATVTTTKRIIVGAHFDTVSGSPGANDNATGTAAALAVARYLKDMTCRKVNVD
                     IVFFDEEEVGLVGARAYSGAITATADVIAVHTLDQLGYDNDNDHRFEIESPTNTLLAE
                     YQAAAGVVGVPVVKTTTMGTDHEAFRDDGFAAVGVTEEYVGGDTTPFRHLSGDTASTV
                     KQSYLQLGAQLAAQVVMTELTP"
     gene            complement(11842..12555)
                     /locus_tag="VMZ53_13025"
     CDS             complement(11842..12555)
                     /locus_tag="VMZ53_13025"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HUS29432.1"
                     /translation="MSRVPLILVATLLSGVAVPVEAAKKPKKHKKQKDDRRVTKKTKA
                     NMPRGFVWPASKNMVAMADACEQELDELGIARQAAKDAGRVVRPMKPDADIGGITYTA
                     VYSKNQVFDCQLVLALAKFAPRLGELGVAEVRFGSAFRWSKVRVNGKTKNILSRHGLG
                     LALDIVSFVDTAGIDHNVKQDYKRGDDLLHEIERSVNDSGQFRLLLTPRNDPKSHSDH
                     FHLEANPDYTETVTERPSS"
     gene            12587..18901
                     /locus_tag="VMZ53_13030"
     CDS             12587..18901
                     /locus_tag="VMZ53_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29433.1"
                     /translation="MDLEILEALALAPDRSQALGQLLPGSEDHDYFRCLHAQHRGALD
                     EAAKILDVWTERHGHTDRYERLRQRQLWYRLDREVDEVSDELRDHFGVDHSHEAEVPE
                     LDPSRPTKLAPGTFDGAALLAEAVEYDNDLTQVTDEGLLELLGADLSSSRRRALLSRI
                     GHSPRPELVALVAADLDVRYFGQLSAHQQLTLDQLHALAARKPELRTHRTWIEFVLRR
                     MQPPVTVDLELDLATRETYIRELWAFVAPLPPAMNSLKAHVLWHLLDTLRRRDVTPDL
                     ALVVMYLQLPRSAGYLPQRWIERIRNDEIAQLGADFRGVTGLPPAGDDEELVRDLLRR
                     NLSSAEQFAQWLERTWLDIEIATAQLLLGVGDPDKATRILGPSAAAALRERVELAWCA
                     HNPKQFRVDEPIVLEADVKHVPELVVKVFRIDPLAYFQHNRREVNTDLDLDGLAASHE
                     QTMHFSEPAVRRVRRRIELPMCARPGTYVIDLIGNGIASRALVTKGRLRYVSRVGAPG
                     TLVTILDEAGRPRPDARAWLGDREYTPDAEGTFVVPFSTSPASQAMLLSCGDIATVAY
                     ISLVSEAYALDLALSLDRQALTDGATARAIARLRLTVAGAPVSLALLEQPQWSVSLTD
                     RQGVTTTKSFPLALSDDNAAVLELPLGEAIAYVSVAVSAKVKVISEQREQDLAQSAYV
                     QVAEMHATTDTEALYLARTSSHGWVLSALGKTGEPRAQRPLTITLVHRWALTELNVEL
                     ATDAYGRCELGELPGISRITAVLGDQTQTWNLLDEPRQLTLNTRDVLIALPPSRTADE
                     VIARASLVELRGGAPARHASATIEPASALFSASGYAGQTTRPDRPNTGQATSGGGTAG
                     GGAAGGGGIVIRGLPPGDYWLRAPGVAATIRVPRAGVDVATSVLTESEVVELTRREPV
                     IADVTISAGGASSGGATSGGASSGGDELVVRVLPASPRTRVHVIATRFIAAPSTALGS
                     VVPAAQYRFDRARGAAYVSGRELGDEYRYILERRTAKRFPSVIADKPSLLLNPWARRT
                     TSTAVAHAATGRAFAASAPAPMAGGYYRQQAQEPAAGNSEAYVAYDFLPERPVVIANL
                     IPDNGVVRVPMSELGRATCVTVIADDVGGGRHVRQAFLRETALEPKDLRLRLALDPSR
                     PTTQQKSIAPLAPAQSLVIADLATAKVHLLDSVEKAHAYLLALRDDPTLREFAFVTRW
                     HSTADAERRELYSKYACHELHLFLYFRDRAFFDAVVKPYLAHKRVKTFVDHWLLEADL
                     RWYLEPARLERLNAVERALLAQRLRADPELARMLADEVLLIPPDPARDTRLIDALLGA
                     SALDGDEQMKGFAADALQMAESAAAPMMELARGGFGAGAPGAAMPPPMPQAAPAKPKA
                     AKKRAMEKMDDADEEVAADEPMDSREMERDMSRRREAAPMFRAQDKTQEWAENNWWHL
                     TPAESSAELIAVNRLWRDFARHEGGAFLSPSLGLATNSFAEAMCALAVTDLPFVVPAH
                     AYNAEGPTLTITAAGNSLVGTSQLVAGELVPGGAPLVVGQSYVRTDDRHQWVDGEQVD
                     KYVEGSFLAGVVYTCQIVLANPSSSRQRIAALVQIPRGSMPLAGARPTHTLDVVLEPY
                     GTHGHEYSFYFPAPGEYTHFPVHVSRGGTIVAAAPSRPLIVTADAEQLDLNSWPHISQ
                     RGSIADVVAYLGRANLAEIELERVAWRIDDRAAYDAILGVLERRHAYDETLWGYALLH
                     NDLPRIKVWARTLGDRLLSAGPVLEMLGIDSEALGSYEHLEYAPLTNARAHRLGSKLR
                     ILNDGLSAQYTRFLELVAHRPAPTAEDVLAFAMYLLAQDRNEAALAALARVDTTAISD
                     KLQHDYLAAYAACLTGEVRRAKELVAMWRGLPVDRWRRKFEAIGAMLAELDGAAPAIV
                     DPRSRDQQQSELAAKQPTFDIALDRDGVVIRQQHVGSLELRFFEMDVELLFSRQPFVQ
                     SDVSRFSFIEPGYRETLTGIPVEHRVPWPAALRGKNVVVEAVGVGQRKAKIHYANDLA
                     TNIAQQYGQVRVQRASDHGSLSATYVKVYARHRGGAVEFYKDGYTDLRGWFDYATLST
                     NDLERVERFAILVVSDQAGSAIFEATPPMR"
     gene            complement(18898..20187)
                     /locus_tag="VMZ53_13035"
     CDS             complement(18898..20187)
                     /locus_tag="VMZ53_13035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HUS29434.1"
                     /translation="MRSLLVPLLLVVASCADDPSPIDDVDGEGDLGLGDRLPDDMKAD
                     GNWGAALTCKPVPNLPKLANPKLTISLDGLTLHLVDVGGTYDKVFPIGPGKVDTTTTD
                     LEYRESLSLRPVLSRGTGDFKITPSSIQPCKTWWTDPETGEKSPVFAGLPFMSWSGNY
                     AIHGPIDNFRAENGGTLRRGYVSHGCIRMEAADVLEVYARIKGIASVPVHVQRERERR
                     GDGSVVDVASKWIGAACSSDAECGYTNGFCATNPLSQRGFCSARCSGLCADKKGYPST
                     FCVADPATPGQGMCVAKVQSENFECRPYDHFAAARTARFNQPAISANVCMPKSPGWVG
                     DHCRTTSECQTGTECRGATATAPGICSMACTSLCADQPGWADTTCAAVPSLGSGGSCV
                     RTCTPSSNGSECPRDMTCGTVQRFGTTTPRNVCLPAR"
     gene            complement(20314..20784)
                     /locus_tag="VMZ53_13040"
     CDS             complement(20314..20784)
                     /locus_tag="VMZ53_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29435.1"
                     /translation="MIRRSLLHIVSFVLLSLLAGMAHADTAKEAQAVTDEAMARWNAA
                     ASERGIFAKVWDLIDSTAWDNMKRGKESVLDRKRDIDKRLKNHEDVDAELRLADLKRD
                     AKMMDHEIAAYRDAMSSTVWKLQAGVALFIIGVFGASIWRFLKSRQRRYRPLQR"
     gene            20806..21222
                     /locus_tag="VMZ53_13045"
     CDS             20806..21222
                     /locus_tag="VMZ53_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HUS29436.1"
                     /translation="MFHRGELDVATKGRGTYDITQQVEDIVRASKAREGLATVFIHHT
                     SASLIICENADPSVRRDLETFAAKLVPDGDRMFTHDAEGDDDMPAHVRTVLTQTSIGI
                     PIVGGKLALGTWQGLYLWEHRTSPHRRHVTITVIGE"
     gene            21215..22012
                     /locus_tag="VMZ53_13050"
     CDS             21215..22012
                     /locus_tag="VMZ53_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29437.1"
                     /translation="MSELHTRLRQILAALTASDPACKRFGAAQHRYELLPPVTDIELS
                     QIDERLAATDAAPSGVLPEDYADYIARFSAGGVGPYYGLLRADRAAAFLIDAPSTVTA
                     WKRALPIAHVGCGYAAVMPLDGAAAGQIWIDARSLQLVAPVRPSFTAFYLDWIDRLAQ
                     PQGLDPFVPPNQCAVVAALTGYLAMTEQQRGLAAGSLDGEALRETLGELGVGAIEVTA
                     SAPLPIFHAGDRVDPCVACARALQGFAEQGLRLDVVAPGLPPLPARN"
     gene            22068..22598
                     /locus_tag="VMZ53_13055"
     CDS             22068..22598
                     /locus_tag="VMZ53_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29438.1"
                     /translation="MVVFSNHRLGPSAEVLVSDDVARAVLGATADAARPFAEARWEIE
                     LVMWLDRRGDQIGEAAARSEGATRESSRGERSDRGGRTGRAGEDGGGGGAGAGGRRGG
                     GGGGGHVLDVADIAWTPEHFEAQRRFLINAIDRAAASEGGGAHGLAFDRWRRMIEAHP
                     ADSVQVGRRWQWQPTA"
     gene            22720..23691
                     /locus_tag="VMZ53_13060"
     CDS             22720..23691
                     /locus_tag="VMZ53_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29439.1"
                     /translation="MAEAMVARGFYPQPNEWTCGPFALKHALLALGRMVDVKQLATTA
                     KTHWWSGTDEIQLARAAREFECDLVLERRSDPEQARKLLVHYLREQTPVLLCVDEWTH
                     WITVLRAEDRRFVVVDSNDDPLLSVRTWPQLRNWWKYRDVDYNKHEPPVLYDLMAVTP
                     RFRTTVKADFSVERVKFLRRPENRRLARHWNEYLEDLLEICKPPSVRIAQPLSMGEFL
                     RRHAELLMTRVVYWHGDVNRDEVARLLRDLRFVSETYGLVIPASMSRRALGDLAILVS
                     LWVCADRGVDPMFGATGAEVRPVTRSRSKKNGRANGRANGRANGKRH"
     gene            complement(23719..>24011)
                     /locus_tag="VMZ53_13065"
     CDS             complement(23719..>24011)
                     /locus_tag="VMZ53_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HUS29440.1"
                     /translation="VVTRFFTDAPTPGSPWRLGWDAPSHTPGVSQAGDRWPRTGAVGH
                     TGFTGTSVWLDIAHRRWVVLLTNRVHPSRHGTTAEAIKSLRRAIHDAVVDGL"
BASE COUNT         4041 a         7747 c         8065 g         4158 t
ORIGIN      
        1 tggcatccga cgaagcaaga ggtgtcgaaa gacgccgaca gccgcccgcc agtctgactc
       61 gctgtcgaca acgagcccgt cgcgattcta taggtgagaa acgaggccgc tcgtcacgat
      121 cgtgcgaccg acgtggcgct gctcgatctc cgataggccc gagcacaacg cgcgacgact
      181 cgcctcgggt gcgtgaccac gatcgacgaa gacaagtgcg ccgccatcgc gaacgacgcg
      241 acgccattcc ttgagcgtca gctcccagcc atcgtcggtc gcgatgtcga cgccgattac
      301 gccggccagc gagccgtcgt cgatgctcga gagatcagcg agtgcgttcg tgagcttctt
      361 cgacgcgcgc gcgggcatcg cgatcggcag gacctcgcgc ttcgtttcgg ccagtgccgt
      421 tgcgagcttt gcgttgccga tcacggcgag ctttccctcg gcgtttgcgg acgagagggc
      481 ctcgacgatt gccgtgacag cgggcgttac gaagaggcca tcgagcacgg acgggaccgt
      541 actacaggtc gcggaggtac tcgcagcgcg gcgcttctat gtgcggcgtg atccagttga
      601 cgccgagcac ttggtcgagc aagtagcgga agtttccgta cgtgacgacg aagaagtcca
      661 tcttgcgccg tttgatgccg ccacctgtgt cgtccgcgcg aacgcagccg tcgtggatcg
      721 agccgtcggg gagcgtgagg ccgtcgaatt cggggatgta gagcggctcg ccgatcggga
      781 tgaggcgctg gtcgacggcg acggacttga acggcgtgag cggccgcttt ttggcgccgc
      841 gaccgaatgg gtgctctttg acgttgagct gctcgaagca tgtgccgtag ccgtagtcgc
      901 acttgccggc gtagctgatc acgcggccgt cttccatgag cagcgagcct tcgaggcgca
      961 cggcccacgc gagcttctcc gtcacgttgc cgaggaacca gcctttgggc gtgtagagat
     1021 cgacgcgcgc gtcttcggag actcggtcgg cttcgccgcg gccgcttttc ggggcgttcg
     1081 ggctcgccgg ctcgttgtag tcgcgctcga gaaccatcca gtagaagcgc agcgcccacc
     1141 gttgatcgtc gacgagcgga tagccgcagc agagcttgtt cttgccgcga agcggggctt
     1201 tgggctggag cgggacggct tctttcgcga gcttgctgtc gtcggcgcgg gctgtgggcg
     1261 cgagcacggc gagcgcgacg agagttgcgg caaagcgcac gaaacgaggg tactgtgatt
     1321 cgcgtgtggg tgcacgatct gcgcgatgcg cacgacgcga ggtgtggcgg caaggcgcgc
     1381 ggcctcgcga cattgatcgg tgcgggcgtc gatgtgccgc ccggcttcgt gatcgatgat
     1441 cgtgcgtttc gcaacatcgt cggcgagctg aagatcgatg acgtcgggga tatcgggcat
     1501 gcgctcgcgc aagcggcgga gcgaattgcc agcgcgcgaa ttcccgacga gctgatggca
     1561 gcggtcaacg atcgcgttgg cgagctcggt catctgctgg cagtgcgctc gtcggcgacg
     1621 atcgaagatc aggcggcagg tgctgcggcg ggcgtgttct cgtcgcgcgg ctccgtgcac
     1681 gtcgatgagc tgtgggatgc gattcgcgcg gtgtggacga gcgcgctcac gccgttggcc
     1741 gctgcgtatg cgcgacgacg cggcggtgag ctatcgatcg gcgtcatcgt gcaagagttc
     1801 gtcatcggcg tgccgaccgt cgtgtacacg cggccgcctg gacagccgga gtcacacgag
     1861 atcctggtgc agcgcaacga tcacgtgtca cgacactcgc gcgctgatct gcctcgcgag
     1921 atcgagcatc agcatgcggc cgtgctcgcg atgcgcgtcg agcagatctt ggcgatgccg
     1981 tcgggcgtcg acgtcgagct cgtccagatc cgaaagcagt cggggttcga cgtcgcgatg
     2041 gagacggtcg tcgtgcaggc gcggccgatc gtgcatccga aaccgaatgc gctcacgcca
     2101 ccgccgccca atgtgctcgc ggcactcgat gacgggcgta cgtggcgatg ggacgtcgcg
     2161 cacaatccag atccactctc gccggcgcag cagggactcg tcgagcgcgt cgagaggacg
     2221 ggcatcgcgc cgtggtccgt gcgcgtggtc gccggcttcc tttattcggc gtcacgcggc
     2281 gagcccgcgc cgtcggtcgg agaaccggct gcctttgctg ctcgcgcggc ggagctcgaa
     2341 gcgcgcctcg agaagacgct gcacgtcgag ggcacaccga cggtcgccga agccatcgag
     2401 cgctacctcg cgttctatgc gctgtgggcg cgcgagctat cgccgctcat cgcggccgcg
     2461 aaggccgcag ctcccgcgag tgcgttcgtc gtcggtactc ggccgtcatc ggtcgaagcg
     2521 acattgctcg cagcggcgcg cggtgacatc agcgaggaag acgtcgaacg tcagctcggc
     2581 tgtctggcgc ccgcgtggga cgtcgccgtg cggacctatg cggagcgacc cggcgttctg
     2641 cgcgatgccg tcgcgcgagc gagagtcgcg ctgaccaagc acgccgacat cgtcccgttg
     2701 cccgtcgagc aaactccgga gatgaagctg gcgcgactgg ccggtgagct cgccgagcgc
     2761 gacgacgcct tgttcgcgcg cgcgcaactg ctcgttcgca aagcgttgct cgctcgaggc
     2821 aaggaggccg gcgtcggcga ggacatcttc tggctcccac tcgacgaggc tgcagcgaca
     2881 aaagcgctcg atccgatcga cgctcgccga cgagccgccg gtgcccgcgc tgccgcggag
     2941 cgcgccgctc gttggcagat gccgctcgtg cacccgacga cggaacagcc cgcgaccgag
     3001 cagagcagtg cgatacaact tcgcggggtt ggaacgggtc cgcgcgtcgg cggccgggtc
     3061 gtccgcttcg cctcacttgc gagcgcgatc gccgtcggcg ctggagatgt cgtcgtcact
     3121 cgcgcagtta cgccggctct cgcggtactc gtcgcaggct gcgccgcact cgtgagtgaa
     3181 agcggcggct tgctcgatca cggagcagcg ctcgcgcggg agttgggcat cccgtgtgtc
     3241 gtcggctgtc gcaacgcgtg gtcgcaacta gcggacggca tgttggttac cgtcgacggc
     3301 gaaggcggaa cggtgacaac gacttattcg tcgtcgtcgt cgtaatcgtc gggttcgttc
     3361 gggtccgtat cgtcgggctc gtcaggcttg tccgctggaa gcggcgtgcc gagaccggcg
     3421 ttgactcgtt cgcgcaagtc tttgcctacc ttgaagaaag gcaaccgctt ctcggcgacg
     3481 tgaacggtct cgccggttcg cgggttgcgc ccttcatagg ctttgtattt tcgaacggtg
     3541 aagcttccaa agccgcggat ctcgatacct tccccgcgtt ctagagcctt caccatcgag
     3601 tcaaagatgg tgttgacgat cgcctccgcc ttaccagccg gcagcttcaa cgtctcagat
     3661 atcttctcga tgagctcgga cttggtcacc cgtaactccg cgtaagtacg gaatcaggct
     3721 aaacgccctt ggaggtccgg cgcaagtcta tctatgcgga aactcgtctt ttcatccgtc
     3781 gaccgctggg atggcacgct cgccatcgct cttaagcgct cgatatgtca ccggaaactg
     3841 gtaactttgg cagcggtgac gcgcatagaa atcgacgaga tcccgccacc ctagatcgcc
     3901 ccgttcgagg cccgttcgga ccaccgcggg catgatttga gagcaagtct cgctcgtctg
     3961 taccccgtga tgcccccggg cgatccggtc ttcgacgccc acttgctgcg gcgagacctg
     4021 gctcagatac caggccgaca gcggcaacac ctcgagcgtc tcgaccttcg tcacggtgat
     4081 cgacatgccg tcgcgccaca gacattcggt gcgccgctcc gacgagccct tgctgatcac
     4141 catcgcgttg aagggcagcg ccggcgcctt cgtgcactgc gacgagccgc tgctcaccga
     4201 ggtcgtgacg ttataagcga cgtcgctgtc gagcttgttc tcgatgacaa gcacgacctg
     4261 atcgctcgag cgctcgaggt gcaggcgaag tccggcaaga tcgatcggcg gcttgccggg
     4321 ctcgagcaca tgccgcgcgt cttccacgtg atacgcgagc ttcttcgaca tgtcgctaac
     4381 actcggagag ctcacgcctg cgttctgcgc gtcgctatcg agaaagctct tgtagacgga
     4441 cttcgaaccc gtcgacttgt tgagcagctc gcgctgcgtt ccactcggtc ccgtcggcgg
     4501 cggcggcttc gacggcagcg aaatactccc gcagaaccgc agatagacaa tcaatgcgac
     4561 gaggaagaac gcgcccgcga gtgtccccaa gcggaactgc gaatccgaca tccacttcac
     4621 catacacctg gaactgcgca gttcgcgcag ggctacgcag gtagaaaaca ttgacttcgt
     4681 tctgcggcca agcgcctaat cgaaccacga gcatgttcgg cagagggaac cggatcgcaa
     4741 agctcgtcgc agtcgcggtg ctcgcgcttg ccgtgccgca ccgggcgcat gctgcagatc
     4801 gcgtcaccga gctgacgtcg atgctatcga gctcgagcga aaagacgcgc atctcggcag
     4861 tgctctcgct cgcacgcctc ggtgacaaga gcgcgctcaa gcctctcgtc acggcgcttc
     4921 acgatccatc tgcgcaggtc cgcgcgatcg ctgcgaccgc tctcggcaag ctcggccaca
     4981 aggctgcgtt gcccgcgctc aagagcgctg cgacagatga tgtcgacgag accgttcgtc
     5041 agaaggccgc cgaagcagca gcgagcgtag cgaagctcaa ccacctcgac gagtcgcccg
     5101 ccgagaaaga tgcgtccgca caagcgcgcg tccagcagaa gacgtcgggc ttcggtcgct
     5161 cgccgcacgc ggtgacggat cgtcccgacc tgttcatcac ggtgaaaggc gcgaacgacg
     5221 attcgcccgg cacgaccgac aaagcgacgc gcaagctcaa cgccgacctc ctcaaagcat
     5281 cgctgcagac ctcgctcgcc aaagcgccgc aggtcacgat ggcgcccgac gaagcagggc
     5341 gctggggcct cgatgttcgc agcgtcgaca tcagcgtcgt gaagctcgag ctgtcgcaag
     5401 acggcaacta catggtgatg gccgcggagc tgcgcctcgc gatctccgac gacaagggca
     5461 agatgctctc gttcttgtcg ggcggcgcga aggtccaaat cccgcgcagc aagtacagcg
     5521 cgaagatcct gccgaacatg cgcaaagacg ccctcgaagg cgcgatgcaa ggaatgttcg
     5581 acaagctgct cgttcacctg cggcagatca cgacgacctg agctaaaaca gccgcgcaat
     5641 gcgcgtcgtc ctcgtgctgt tgatcgccct ctgtgcggtc gctcatgcgg acaaggtcat
     5701 tacgaagcag ccgaagctgc tgcaggcggt cgccccggag tacccgccga aggcactcga
     5761 agacggcaag caagcgaagg tcaaagtccg catccatatc gatgcgaccg gcatcgtcac
     5821 gaaggtcgac gtcctcgagc ccgtcggcga tggcttcgat caagctgcga tcgacgcggc
     5881 gatgcaatac gtgttcgagc ccgccgagat cgatggcaag cccggcgaga tcgcagtcga
     5941 gacatcgatc aacttcgtaa tcgagcagcg gcccgaggaa gagccgccgc cactgccgac
     6001 gccgccgcag gagcgcgaag gtccgccgaa tcacgcgggc gcgatgagcc agccgatcac
     6061 gctgcaaggc gaggcgatcg agcgcgggac acgtcgacgg ctgcccggtg tgatcgtatc
     6121 gattgcagag ctgaagctcg acgcggtcac cggcgaggac ggcacgtttt acttccatgg
     6181 cgtcgccccg ggtcactatc agctgctcgc cgtcgatccg aagttcgaca agttcgagcg
     6241 ccccgtcgtg atcgagaagc gcgagcagct cgaggtgcgg ctgtggatgc gcccgcgtgg
     6301 tggcaatcca tacgagacga tcgtcgaggg cgagcgcgaa cagctcgagg ttacgaagcg
     6361 cacgctgcac cgccagcagc tcacgagcgt gcccgggaca tttggcgatc cgattcgcgt
     6421 gattcagacg ctgcccggca tgcagcgcgc gccgttcggg ctcgggctct tgctcgtgcg
     6481 cggcagcaat cccgacgaca ccggcatctt cgtcgacggc cacgaggtac ctgccctatt
     6541 ccacttcctc ggtggtccat cgatcttcaa cgcggagatg ctcgagtcga tcgatctcta
     6601 tcccggcggc tttccgtcga ggttcggtcg ccatcacggc ggtgtcgtcg cgatcgagac
     6661 ccggccgacg aagagcgacg gcattcacgg ctcggcgaaa gtcgacctca tcgatagcgg
     6721 cggctacatc cgcgcgccga tcacgaagaa tgtctcggcg gcgatcgccg gacgtcgctc
     6781 gtacatcgac ttcatcctgc cgctgtttct gcccgagcca tccgccggct cgcagcgcat
     6841 cgtcacgccg gtctactacg actaccaggc gcgcctcgac tggaatctgc acgagaacgg
     6901 ccgcctctcg acgttcttga tcggctcgtc cgacaagctg cacgtattcc agaaagacgc
     6961 cgatacgatg acgtcggcga atctcgacac ggcggtgaaa ttcttccgcg tgatggcaac
     7021 gtacgagcgg cgactcggca aagacttgaa gctgacgcta tcgccgtcgt ggggacagga
     7081 ctcgctcacg ttctccggtg cacaagcaga gaatacggga ccgttcacga gcctcacgct
     7141 gacgaacaag gcgctgtcgt atcggttccg cgtgcacggc aagctgcggc catcactgac
     7201 gatcgacacc ggcatcgatc tgctgtcgcg agcgacgaag ttcgacgcgc tcgttcccgt
     7261 cgacgacacg ctgatcgatt cgagtggcgt cgatgtcgag ccgtcgaagc tgctgcgcgg
     7321 cacgtcgacg atcggaattg cgacctatgc agatgtcgcg atcgatgcga cgtcgaagct
     7381 caagctcatt cccggtctgc gactcgacgg ctacttcatc aacgggcagg agcgttcgtc
     7441 ggtcgaccct cggctcgtcg ttcgctacaa gacggcgccg caatggacgg tgaagggcta
     7501 cgtcggcaag ttcacgcaac cgccacagcc cgagtctctc gactcgcgat tcggcaatcc
     7561 gaaggtcggg ctcgagcacg gctaccacta cggccttggc tacgagtgga agcccgagcg
     7621 tctgtggtcg atcgacagcg agctctatta cgtcgaccgc cgtgatgtcg ttgtgttctc
     7681 gcccgatgtg atcgacaacg gggatggcac gttcacgtat gagaacttca ccaacaatgg
     7741 gcggcgtgcg tcgtatggtt tcgaagcgat catcaagcgc gagatcagcg agcgtgcgtt
     7801 cggttggctc tcgtatacgt acagccactc gcggcagacg aatcggcgcg gcgagaactg
     7861 gttcccgacg ggcttcgatc agccgcacgt gctcaacgcc gtcgcgagct tcaagcccgg
     7921 cggtggttgg gagctcggca cgcgattcca gctcgcgagt ggtcgccccg atgcggtcgt
     7981 gatcggctcg acgttcgacg ccgattgcgg ctgctacgac gctgtggtca ccggcaaccg
     8041 cgcgacgcga gtacccacgt atatgcagct cgacgcgcgc atcgagaaga cgtggctgtt
     8101 cgagctgtgg agcatcggcg cgtatctcga cgtgatcaac gtgacgaatc gcaagaacca
     8161 agagggcatc gactacgact atcgctaccg ccagtcgtcg ccgatcacga gcttcccgat
     8221 ctttccgacg atcggcgtga ggggccaatg gtgaagcgcg ccgtcctact gctcgcgctg
     8281 tccgcgtgca cgacgttcga ggatccggac atcgtgctcg acttccgcgt gctcggcgca
     8341 caagcggaga ttccagagca gatcatcgac atcgacatca cgatgccgcc gaaggccgac
     8401 gacgtgctcg cgcagctcgt cgatacgcgc gtgtgctggc tgatgtccga caagaatttt
     8461 gatcggtcgc tgcgctggtc cgcgacactc tgcaatctca acggtgacga gcgctgcccc
     8521 gaaggctcgc cgcaggcaaa cctcggctcc ggcgtgtggg acgatccgga cacagctccc
     8581 acgcccgaca gcttctgcac gaacgtgccc gcgaacggca acttgctcgg cgtcgcgctc
     8641 gactatctcg agaacgatca gctgcacggc ctcggcggca tctactacgg cgtgtcgctg
     8701 cgcgtcggtg gtgtggacgg cgatccggcg ctcgatctct acgccgcgaa gtcgctgcgc
     8761 atccagccgc gcatcccgcc ggagctgacg gcgaatacga atccgacgct cgtgcagatc
     8821 gaggcgtccg tcgatggtgc agccaacgtg ctgctcgtcg atgcgcgatg tgtcgaccaa
     8881 ccggcgccgc tcgaggttgc ggccggcgcg acgctgcgct tctatcccgt cgagcccgac
     8941 ggtgttcgcg agacgtacgt cgtgccgacg atcgacggca tggttcgcac gttcaccgaa
     9001 agcctcacgt accagtggct cgccacagcc ggaaactacg caggtggccg ctccggcggt
     9061 acgcgcgacg cgttcggcaa tccggcgacg ctatggaccg actggaacgc gcccgacgcc
     9121 gaggatctcg aagggccgct cgatgtcgac ttctggatca tccaacgcga cgaacgcctc
     9181 ggcctgaagt ggttccgcgg ctgcgttcgc gtcgtgccct agatacaagc gtcgcagagg
     9241 ccgcggacgt gcacttcgac cttgcgctgc ttgattgcgc gaggcgcttt cgcacgccgc
     9301 accgcaagct cgatctcggg caagcactcg acagtgccgc actccgtgca gacgaaatgc
     9361 ggatgctcgt gatcgccatt gatcgcttcg aaccgccaga tgtgatcgcc gatatccgtg
     9421 cggcgcacga ggccaacttc ggcgaggtcc gtcaggttgc gatagatcgt cgcgcgatcc
     9481 catgacgacg cggacaaccg atcggcgacg tcgccgtgcg acatcggcac gtcggagaca
     9541 cgaagcagct cgagcacggc gagcctcgac ggcgtcgcgc gtaaaccctt cgcgcgcacg
     9601 gtttgctgca gctccgcgcg cgtgtcgatg ttcatgcgag tagccatgac ggtcgagctt
     9661 accgcattcg ggtatgctcg ggtcgatgtc cttgcggtcg cacttcgaga cgttggtgcg
     9721 aacggagccg cccgaagtcg tcgcggatcg aggcgatgaa gtcgacccgc acagcgtcgg
     9781 cctcgatcgc aagatcatcg atgcgatctg gcaagcgacg atggcgacgt acagaacggg
     9841 gctctaccct gctctctcgc tgtgcattcg ccgtcgcggc gaggtcgtgc tcgatcgctc
     9901 gatcggtcac gtgtcgggaa acgcgccgca cgatccgccg gactcgcgca aagtgatcgc
     9961 gtcgccgaag acgctgttca acatgttcag cgcgtcgaag atggtgacgg cgatgctcgt
    10021 gcatctgtgc gacgagcgcg gcctgttgca cctcgacgat ccggtcgccc gctacctccc
    10081 cgacttcggc gagcacggca agcaccgcat cacgatccgc cacgtgctca cgcatcgcgc
    10141 aggcatcccg cacatcccgc cgcaattcgc ggatgtcggc ctcctcgagc ggcccgacga
    10201 gatcctctcg ctgctctcgc gacaaaagcc gcgctggcgt gccggccaac gcctcgccta
    10261 tcacgcgctc acgggcggct tcgtgctcgg cgcgatcgtc gagaaggtga tggggaagcc
    10321 gctgcgccag gtgatgcacg acgagatgct cgggccgctc ggcttcgacg cattcaactt
    10381 cggcgtcccg caagcacgcc tgcacgaagt cgcgatgaac gcgttcacgg gcccgccgat
    10441 cctgccgccg atctcgcagt tgttgaagcg aatgctcagc gtgcacttcg tcgaggcggt
    10501 gcgcgtgtcc aacgatccgc ggtttctcac gtcggtcgtg cccgcaggca atatcgtgac
    10561 gaccgcgaac gaggcgtcgc gcttcatgca gctgctgctg aacggcggcg agctcgacgg
    10621 ccatcgcgta ttcgagacgc ggacgatcca gcgcgcagtc gccgagcaga cgtacttcga
    10681 gatcgacctg acgatgggcg cgccgatccg ctactcgatg ggcttcatcc tcggcgcacg
    10741 attcgcctcg ccgtacggcc tgaacacgtc gaaggcattc ggccacctcg gcttcacgaa
    10801 cgtgttcgtc tacgcagatc ccgcgcgcga tatcgcggtc ggcttcatga cgtcgggaaa
    10861 gccggcgctg tcgatcggca tcacgcgcac gctgctgacg atgcagacga tcgcccggcg
    10921 aatcccgcgc gacggccgcg gcccgctacg gcgttagctc ggtcatgaca acttgcgcgg
    10981 cgagctgggc accgagctgc aggtatgact gcttcaccgt cgaggcagtg tcgccggaca
    11041 agtggcggaa cggcgtcgtg tcgccaccga cgtattcttc ggtcacgccg accgccgcga
    11101 agccgtcgtc gcgaaacgct tcgtgatccg tgcccatcgt cgttgtcttc acgaccggca
    11161 caccgacgac gcctgctgcc gcttggtatt cggcgagcag cgtattcgta ggcgattcga
    11221 tctcgaagcg atgatcgttg tcgttgtcgt agccaagctg atcgagcgtg tgcaccgcga
    11281 tgacgtcggc ggttgccgtg atggcgccgg agtacgcgcg cgcaccgacg aggccgactt
    11341 cttcttcgtc gaagaacacg atgtcgacgt tgaccttgcg gcacgtcatg tctttgagat
    11401 agcgcgcgac cgcgagcgcg gcagcagtgc cggtggcgtt gtcgttcgcg cccggcgagc
    11461 cggatacggt gtcgaagtgc gcgccgacga tgatgcgctt cgtcgtcgtg acggtcgcgg
    11521 gaatgcgtgc gacgacgttg gcgccggtcg agtacgcggc gagcgacgga gaccagccca
    11581 tctgcgtcag ctgcgtctgg aggtagttgc gcgcggccgt gcgttgggtg cccgtggcgc
    11641 gaggtgcggg gatgttcgtg atgacgtcgt tgagcagagt cggcagccac gtctgtgcga
    11701 gggctggacg catgcagccg cccgcgccat cggagagcga gccgtcgctg gagccagtgg
    11761 gcgcgtcggg cgtcgtcatc atgctgtcgt ccgcgccgca cgctgcggtc agaagcagcg
    11821 ttgccgcgag cgcagcgcgc atcacgacga cggacgttcc gtgaccgtct cggtgtagtc
    11881 cgggttcgct tcgaggtgga agtgatcgga gtgcgacttc ggatcgttgc gcggtgtgag
    11941 gagcaggcgg aactggccgc tgtcgttgac cgagcgctcg atctcgtgga gcaagtcgtc
    12001 gccgcgcttg tagtcctgct tgacgttgtg gtcgatgccg gccgtgtcga cgaacgagac
    12061 gatgtcgagc gcgaggccaa ggccgtggcg cgagaggatg ttcttcgtct tgccgttgac
    12121 gcgcactttg gaccagcgga acgcgctgcc aaacctcact tcggcgacgc cgagctctcc
    12181 gagccgaggc gcgaacttcg cgagtgcgag cacgagctgg cagtcgaaaa cttggttctt
    12241 cgagtagacg gccgtgtacg tgatgccgcc gatatcggcg tcgggcttca tcggacgaac
    12301 gacgcgaccg gcgtctttcg cggcttgtcg cgcgatgccg agctcgtcca gctcctgctc
    12361 gcaggcgtcg gccatcgcga ccatgttctt gctcgcgggc cagacaaaac cgcgcggcat
    12421 gttcgccttc gtcttcttcg tgacgcggcg atcgtctttc tgtttcttgt gcttctttgg
    12481 tttcttcgca gcctcgacgg gaactgccac tcccgacagg agtgtggcta cgagaatgag
    12541 aggcacccgc gacatcgccg cggaagttac cagatacgct ggcgcgatgg acctcgagat
    12601 tctcgaagct ctagccctcg cgcccgaccg gtcgcaagct ttgggccagc tgctaccggg
    12661 cagcgaagac cacgactatt tccgctgctt gcacgcgcag catcgtggtg cgctcgacga
    12721 ggccgcgaaa atcctggacg tctggacgga gcgtcacggc catacagatc gctacgagcg
    12781 gctgcgccaa cgtcagctct ggtatcggct cgatcgcgag gtcgacgagg tctccgacga
    12841 gctgcgcgat catttcggcg tcgatcactc gcatgaagcc gaagtgcccg agctcgatcc
    12901 gagccgaccg acgaagctcg cacctggcac attcgatggc gcggcgctgc tggccgaggc
    12961 cgtcgagtac gacaacgacc tcacgcaggt caccgacgaa ggtctgctcg agctgctcgg
    13021 cgcagatcta tcgagcagcc gccggcgtgc gctgctgagc cgtatcggcc actcgcctcg
    13081 gcccgagctc gtcgcgctcg tcgcggccga tctcgacgtt cgctactttg gtcagctcag
    13141 cgcgcatcag cagctcacgc tcgatcagct gcatgcgctc gctgcgcgca agccggagct
    13201 gcgcacgcat cggacgtgga tcgaattcgt cctgcgcagg atgcaaccgc ctgtcaccgt
    13261 cgatctcgag ctcgatctcg caacgcgcga gacatacatc cgcgagctgt gggcattcgt
    13321 cgcgccgctg ccgcccgcga tgaactcgct gaaggcgcac gtgctctggc acttgctcga
    13381 cacgctacgg cgtcgggacg tgacgccgga tctcgcgctc gtcgtgatgt acctccaact
    13441 gccgcgctca gccggctatc tgccgcagcg ctggatcgaa cgcattcgca acgacgagat
    13501 cgcgcagctc ggtgccgact tccgtggcgt gacgggtctg ccgcccgctg gcgacgacga
    13561 agagctcgtg cgcgacctcc tgcgccgcaa cctcagcagc gcggagcagt tcgcgcagtg
    13621 gctcgagcgc acgtggctcg atatcgagat cgcgaccgcg cagctgctcc tcggcgtcgg
    13681 agatccagac aaggcgacgc gcatcctcgg ccctagcgct gccgcggcgc tgcgcgaacg
    13741 cgtcgagctc gcgtggtgcg cgcataaccc gaaacagttc cgcgtcgacg agccgatcgt
    13801 cctcgaggcc gacgtcaagc acgtgcccga gctcgtcgtc aaagtgttcc ggatcgaccc
    13861 gctcgcgtac ttccaacaca accgtcgcga ggtgaacacc gatctcgacc tcgacggtct
    13921 ggccgcgtcg cacgagcaaa cgatgcattt cagcgagccg gccgtgcgcc gcgttcggcg
    13981 tcggatcgag ctgccgatgt gtgcgcgtcc cggcacgtac gtgatcgatc tcatcggcaa
    14041 cggcatcgcg agccgcgcgc tcgtcacgaa aggccgcctg cgttacgtgt cgcgcgtcgg
    14101 tgcgccgggc acgctcgtca cgatcctcga cgaggcaggc cgcccacgtc cggacgcgcg
    14161 cgcgtggctc ggcgatcgcg aatacacgcc cgacgccgag ggcacattcg tcgtcccatt
    14221 ctcgacgtct ccggcttcgc aagcgatgct gctgtcgtgc ggcgacatcg cgacggtcgc
    14281 gtacatctcg ctggtctccg aggcgtacgc tctcgacctc gcgctctcgc tcgatcgcca
    14341 agcactcacc gacggcgcaa cggctcgcgc gatcgcacgc ctacgcctca ccgtcgccgg
    14401 tgcaccggtc tcgctcgcac tcctcgagca gccgcaatgg agcgtgtcgc tcaccgatcg
    14461 ccaaggggtc acgaccacca agtccttccc gctcgctctc agcgacgaca acgcggcggt
    14521 tctcgagctg ccgctcggcg aagcgatcgc atacgtcagc gtcgccgtca gcgcgaaggt
    14581 caaggtcatc agcgagcagc gagaacaaga cctcgcgcaa agtgcgtacg tgcaagtcgc
    14641 cgagatgcac gcgacgaccg acaccgaggc gctctacctc gcgcgcactt cttcgcatgg
    14701 ctgggtgctg tcggcgctcg gcaagacagg cgagccgcgt gcgcaacggc cgctcacgat
    14761 cacgctcgtg catcgatggg cgctcacgga gctcaacgtc gagctggcca ccgacgcgta
    14821 cgggcgctgc gagctcggtg agcttcccgg catctcgcgc atcacggcgg tgctcggcga
    14881 ccaaacgcag acgtggaatc tcctcgacga gccgcgccaa ctaacactca acacgcgcga
    14941 cgtgctgatc gctctgccgc cgagccgcac tgccgacgag gtgatcgcac gcgcgtcact
    15001 cgtcgagctt cgcggtggcg ccccggcgcg tcacgcatcg gccacgatcg agcccgcctc
    15061 tgcgctcttc tcggcctcgg gctatgcggg tcagaccacg cgacccgatc gtccgaatac
    15121 cggacaagct acgagtgggg gcgggaccgc gggcggaggc gcggcgggcg gtggcggaat
    15181 cgtgatccgc gggttgccgc cgggtgacta ttggctgcgc gcgccgggcg tggcagcgac
    15241 gattcgcgtg ccgcgcgcag gcgtcgacgt cgcgacatct gtgctgacag agagcgaggt
    15301 tgtcgagctg acgcggcgcg agccggtgat cgccgacgtg acgatcagcg cgggcggcgc
    15361 gtcgagcggc ggagcgacga gcggtggagc gtcgagcggc ggcgatgagc tcgtcgtgcg
    15421 ggtccttcct gcgtcgccga gaacgcgcgt gcatgtgatc gcgacgcggt tcatcgcggc
    15481 accgtcgaca gcattgggca gcgtggtgcc ggcggcgcag taccgattcg atcgcgcacg
    15541 tggtgcggcg tacgtgtcgg gtcgcgagct cggcgacgag tatcgctaca ttttggagcg
    15601 acgcaccgcg aagcgctttc cctcggtgat cgcggacaag ccgagcttgc tgctcaatcc
    15661 atgggcgcga cgcacgacga gcacagcggt ggcgcatgcg gcgacggggc gtgcgtttgc
    15721 agcgtctgca cctgcgccga tggcgggtgg gtattaccgg cagcaagcgc aggagcccgc
    15781 agccggcaat agcgaagcgt atgtggcgta cgacttcttg ccggagcggc ctgtcgtgat
    15841 cgcgaacttg atcccggaca atggcgtcgt tcgcgtcccg atgagcgagc tcgggcgcgc
    15901 gacgtgcgtg accgtgattg ccgacgacgt cggcggtggc cgccacgtgc ggcaggcgtt
    15961 cctgcgcgag accgcgctcg agccgaagga tctgcgcctc cgcttggcgc tcgatccatc
    16021 gcgaccgacc acgcagcaga agagcattgc gccgctcgct cctgcacagt cgctcgtgat
    16081 cgcggatctc gcgacggcga aggttcacct gctcgacagc gtcgagaaag cgcatgcgta
    16141 cctgttggcg ctgcgcgacg acccgacgtt gcgcgagttt gcgttcgtca cgcgctggca
    16201 ttcgaccgct gatgcagagc gacgcgagct gtactcgaag tacgcgtgtc acgagctgca
    16261 tctgtttctg tatttccgcg atcgcgcgtt cttcgacgca gtcgtaaagc cgtacttggc
    16321 gcacaagcgc gtaaagacgt ttgtcgatca ctggctgctc gaggcggacc tgcgctggta
    16381 tctggagccg gcgaggctcg agcggttgaa cgcggtggag agagccctgc tcgcgcagcg
    16441 gttgcgtgcg gaccccgagc ttgcgcggat gctcgccgac gaggtgttgc tcatcccgcc
    16501 cgatccagcg cgcgatacgc gcctcatcga tgcgttgctc ggtgcgtcgg cgctcgacgg
    16561 cgacgagcag atgaaaggct ttgcagcaga cgcgctgcag atggcggagt cggccgcagc
    16621 gccgatgatg gagctcgcgc gcggtggatt cggcgccggt gcaccaggag ccgcgatgcc
    16681 gcccccgatg ccgcaggcgg cgcccgccaa gccgaaggct gcgaagaagc gcgccatgga
    16741 gaagatggac gacgccgacg aggaggtcgc cgccgacgag ccgatggact cgcgcgagat
    16801 ggagcgcgat atgagccggc gacgcgaggc cgcgccgatg ttccgcgcgc aggacaagac
    16861 gcaggagtgg gcggagaaca actggtggca cctgacgccc gcggagagca gtgccgagct
    16921 gatcgcggtg aaccggctgt ggcgcgactt tgcgaggcac gagggcggcg cattcctgtc
    16981 gccatcgctc gggctcgcga cgaacagctt cgccgaggcg atgtgcgcgc ttgccgtgac
    17041 cgacttgccg ttcgtcgtgc cggcgcatgc gtacaacgcc gaaggtccga cgctgacgat
    17101 cacggcggcc ggcaattcgc tcgtcggaac gtcgcagctc gtcgccggtg agctcgtgcc
    17161 aggtggcgcg ccgctcgtcg tcgggcagag ctacgtgcgc accgatgatc gtcatcagtg
    17221 ggtcgatggc gagcaagtcg acaagtacgt cgagggcagc ttcctcgccg gcgtcgtcta
    17281 cacgtgtcag atcgtgctcg ccaatccgtc gagctcgcgg cagcgcatcg cggcgctcgt
    17341 gcagattcca cgtggcagca tgccgctggc gggcgcgcgc ccgacgcata cgctcgacgt
    17401 cgtgctcgag ccgtatggca cgcatggtca cgagtactcg ttctactttc cagcgcctgg
    17461 tgagtacacg cacttcccgg tgcacgtcag tcgcggcgga acgatcgtcg cagccgcgcc
    17521 atcacgcccg ctcatcgtca ccgcggatgc agaacagctc gatctgaatt cgtggccgca
    17581 catttcgcag cgaggcagca tcgccgatgt ggtcgcgtat ctcggtcgcg caaacctcgc
    17641 cgagatcgag ctcgagcgcg tcgcctggcg gatcgacgat cgcgcggcgt atgacgccat
    17701 cctcggcgtg ctcgagaggc ggcacgcgta cgacgagacc ctgtggggct atgcgctgct
    17761 ccacaacgat ctgccgcgca tcaaagtctg ggcgcgcacg ctcggcgatc ggctgctctc
    17821 cgcgggacca gtgctcgaga tgctcggcat cgacagcgag gcgctcggca gctacgagca
    17881 tctcgagtat gcgccgctga caaatgcgcg cgcgcatcgg ctcggctcga agctgcgcat
    17941 cttgaacgac gggctctccg cgcagtacac gcggttcctc gagctcgtcg cgcatcggcc
    18001 cgcgccgacg gcggaagacg tgctcgcgtt tgcgatgtac ttgctcgcgc aggatcgcaa
    18061 cgaagcggcg ctggcggctc tcgctcgcgt cgacacgacg gcgatcagcg acaagctaca
    18121 gcacgactat ctcgcggcgt atgcggcgtg cttgaccggc gaggtgcggc gcgcgaaaga
    18181 gctcgtcgcg atgtggcgcg ggctgccggt cgatcgctgg cgtcggaagt tcgaggcgat
    18241 tggcgcgatg ctcgcggagc tcgacggtgc agcacctgcg atcgtcgatc cgcgcagtcg
    18301 cgatcaacag cagagcgagc tcgcggcgaa gcagccgacg ttcgacattg cgctcgatcg
    18361 cgatggcgtc gtcatccggc agcagcatgt cggctcgctc gagctgcgtt tcttcgagat
    18421 ggacgtcgag ctgctgttct cgcggcagcc gttcgtgcag agcgacgtgt cacggttctc
    18481 gttcatcgag cccggctatc gcgagacgct caccggcatt cctgtcgagc atcgcgtgcc
    18541 gtggccggcg gcgttgcgcg ggaagaatgt cgtcgtcgag gccgtcggcg tgggtcagcg
    18601 caaggcgaag atccactacg cgaatgacct cgcgaccaac atcgcgcagc agtacgggca
    18661 agttcgcgtg caacgtgcgt cggaccacgg ctcgctatcg gcgacgtacg tgaaggtgta
    18721 cgcgcggcat cgcggtggcg ctgtggagtt ctacaaagac ggctacaccg atctgcgcgg
    18781 ttggttcgac tacgcgacgc tgtcgacgaa cgatctcgag cgcgtcgagc gattcgcgat
    18841 cctcgtcgta tcggatcaag cgggctcggc gatcttcgag gccacgccgc ccatgcgtta
    18901 gcgagcgggt aagcagacgt tgcgcggcgt cgtcgtgccg aagcgctgca ccgtgccgca
    18961 cgtcatgtca cgcgggcatt cagagccgtt cgagctcggc gtgcacgtgc gaacgcagct
    19021 accgcccgaa ccgaggctcg gaaccgcggc gcatgtcgtg tccgcccagc cgggctgatc
    19081 agcgcacagc gaggtgcacg ccatcgagca gatgcccggt gcggttgccg tcgcgccgcg
    19141 gcactcggtg ccggtctggc actcgctcgt cgtgcggcag tgatcgccga cccaaccggg
    19201 cgacttcggc atgcacacgt tcgcggaaat cgccggctga ttgaatcgcg cagtgcgagc
    19261 ggccgcgaag tgatcgtagg ggcggcactc gaagttctcc gactggacct tcgcgacgca
    19321 catgccttgg cccggcgtcg ccggatccgc gacgcagaac gtcgacgggt agcccttctt
    19381 gtcggcgcac agcccgctgc acctcgcgct gcagaagccg cgctgcgaga gtggattcgt
    19441 cgcgcagaag ccgttcgtgt aaccgcactc cgcatcgctg ctgcaagctg cgccgatcca
    19501 cttcgatgcg acgtcgacga cgctgccatc tccacgacgc tcgcgctcgc gctgcacgtg
    19561 gaccgggacg ctcgcgatgc ctttgatgcg cgcgtacacc tcgagcacgt cggccgcttc
    19621 catgcggatg cagccgtgcg agacgtagcc gcggcgaagc gtgccgccgt tctcggcgcg
    19681 gaagttatcg atcggtccgt gaatggcgta gttgccagac cagctcatga atggaaggcc
    19741 ggcgaacacg ggcgacttct cgccggtctc tggatcggtc caccacgtct tgcacggctg
    19801 gatcgatgac ggcgtgatct tgaagtcacc ggtgccgcgg ctgagcacgg gccgcagcga
    19861 gagcgactcg cgatattcga gatcggtcgt ggtcgtgtcg accttgcccg ggccgatggg
    19921 aaagaccttg tcgtaggtgc caccgacgtc gacgagatgg agcgtgaggc cgtcgagcga
    19981 gatggtcagc ttcgggttcg cgagcttcgg cagattcggg acgggcttgc aggtgagcgc
    20041 tgcgccccag ttgccgtcgg ctttcatgtc gtcgggcagc cggtcaccca gaccgaggtc
    20101 accttcgccg tcgacatcgt cgatcgggga cggatcatcg gcgcatgacg cgacgacgag
    20161 gaggagcggt acgaggaggc tgcgcacgcg cgcccgttgt gcaagcagca caccgcgtca
    20221 cggatcgcgc aactccgcgg cggcaaagct ggctactcga gctgccagct accccggtgc
    20281 tcgggacaac acgcccaacg agcgcgtttg tcctcagcgt tggagtgggc ggtagcggcg
    20341 ctggcgactc ttgagaaacc gccagatcga ggcgccgaag acgccgatga tgaagagcgc
    20401 gacgccggct tgcagcttcc agacggtgga gctcatcgcg tctcggtacg cggcgatctc
    20461 gtggtccatc atcttcgcgt cgcgcttgag gtcggcgagg cgcagctcgg cgtcgacgtc
    20521 ttcgtggttc ttcagccgct tgtcgatgtc gcgcttgcga tcgagcacgg actctttgcc
    20581 gcgcttcatg ttgtcccacg cggtgctgtc gatgaggtcc cagaccttgg cgaagatgcc
    20641 gcgttccgag gcggcggcat tccagcgcgc catcgcctcg tcggtgacgg cttgtgcctc
    20701 tttggccgtg tcggcgtgcg ccatgcccgc gagcagcgac aggagcacga acgaaacgat
    20761 gtggagaagc gaacgtcgaa tcaccgacgg agtataccac cggggatgtt ccaccgaggc
    20821 gagctcgacg ttgcgaccaa aggccgtggc acctacgaca tcacgcagca ggtcgaagac
    20881 atcgtgcgcg caagtaaagc gcgtgaaggt ctcgcgacgg tgttcatcca ccacacgagc
    20941 gcatcgctca tcatctgcga aaacgccgat ccgagcgtgc gccgcgacct cgagacgttc
    21001 gccgcgaagc tcgtccccga cggcgaccgc atgttcaccc acgacgccga aggcgacgac
    21061 gacatgcccg cgcacgtgcg caccgtactg acccagacat cgatcggtat tccgatcgtc
    21121 ggcggcaagc tcgcgctcgg cacatggcag ggcctctatc tctgggagca ccgcacgtcg
    21181 ccgcatcgtc gccacgtgac gatcacggtg atcggtgagt gagctgcaca cgcgcctgcg
    21241 ccaaatcctc gccgcgctga ctgcgtccga tccggcgtgc aagcgctttg gcgctgcgca
    21301 acaccgctac gagctgctgc caccggtgac ggacatcgag ctgtcgcaga tcgatgagcg
    21361 gctcgctgcg acggacgccg cgcccagtgg cgtgcttccc gaagactacg ccgactacat
    21421 cgcgcggttc tccgcgggcg gcgtcggtcc gtactacggc ctccttcgcg cagatcgcgc
    21481 cgcggcattc ttgatcgacg cgccgtcgac cgtgacggcc tggaagcgcg cgcttccgat
    21541 cgcgcacgtc ggctgtggct acgcggcggt gatgccgctc gacggcgcag ctgccggcca
    21601 gatctggatc gacgcgcgct cgctgcaact ggtcgcgccg gtgcggccga gcttcacggc
    21661 gttctatctc gattggatcg atcggctcgc gcagccgcag ggcctcgacc cgttcgtgcc
    21721 gccgaatcaa tgcgcggtcg tcgcggcgct gacgggctac ctcgcgatga ccgaacaaca
    21781 gcgcggcctc gctgcgggct cgctcgacgg tgaagcgcta cgcgagacgc tcggcgagct
    21841 cggcgtcggt gcgatcgaag tgacggcgag cgctccgctg ccgatctttc atgcaggtga
    21901 tcgagtcgat ccgtgcgttg cctgcgcccg cgcgctgcag ggcttcgcgg aacagggcct
    21961 gcgcctcgac gtcgtcgcac cgggcctgcc tcccctgccc gcgcgcaatt aattcagtcc
    22021 gacctaattt ccggggagtg tcacaccggt tgtctagggt gcgggccatg gtcgtcttct
    22081 cgaaccatcg gctcgggccg agcgcggaag tcctcgtttc ggatgacgtt gcgagggcgg
    22141 tgcttggcgc gacggccgat gcggcgcggc cgtttgccga agcgcggtgg gagatcgagc
    22201 tcgtgatgtg gctcgatcgg cgaggcgatc agatcgggga ggcggcggct cgcagcgaag
    22261 gagcaacgcg cgagtcgagt cgcggcgagc gcagcgatcg gggcggccgg acgggtcgag
    22321 ccggcgagga cggcggtggc ggtggcgccg gcgctggcgg tcgtcgcggt ggcggcggcg
    22381 gcggcgggca tgtcctcgat gtggctgaca ttgcgtggac gccggagcat ttcgaagcgc
    22441 agcgcaggtt cttgataaat gcgatcgacc gggcggcagc cagcgagggt ggcggtgcgc
    22501 atgggctggc gtttgaccgc tggcggcgga tgatcgaggc gcatcccgcc gattcggtgc
    22561 aggtcgggcg gcggtggcaa tggcagccaa ctgcctgatc ggtgtgcggg atctgcacgc
    22621 caagtgctcg acaactggtt gattgttcag gagaaagttc gtatcgggag tggcttctag
    22681 gaaccgggcc acgggggcgg tttgagctag agtcgcaccg tggcggaagc gatggttgcc
    22741 cgcgggttct acccgcagcc caacgaatgg acctgtggtc cgttcgcgct gaagcacgcg
    22801 ttgctcgcgc tcggccgcat ggtcgacgtg aagcagctcg cgacgactgc gaagacgcac
    22861 tggtggtcgg gcacggatga aatccagctc gcgcgtgcag cgcgcgaatt cgaatgcgac
    22921 ctcgtgctcg agcgccgcag tgatcccgaa caggcgcgaa agcttttggt gcattacctg
    22981 cgcgagcaga cgccggtgct gctgtgtgtt gatgagtgga cgcactggat caccgtgctg
    23041 cgcgcggagg atcggcgctt tgttgtcgtc gattcgaatg acgatccgct gctgtcggtc
    23101 cggacgtggc cgcagctgcg taattggtgg aagtaccgcg acgtcgacta caacaagcac
    23161 gagccgccgg tcctctatga cttgatggcg gtgacgccgc gcttccggac gacggtgaag
    23221 gccgacttct ccgtcgagcg cgtgaagttt ttgcgccggc cggagaatcg ccgcctcgcg
    23281 cggcattgga acgagtacct cgaagatctg ctcgagatct gtaagccgcc gagtgtgcgc
    23341 atcgcgcagc cgctctcgat gggcgagttc ttgcggcggc acgccgagct gttgatgacg
    23401 cgcgtcgtgt actggcatgg cgacgtcaat cgcgacgagg tcgcgcggtt gcttcgcgat
    23461 ctgcggttcg tgtccgagac gtacggtctg gtgattccgg catcgatgag caggcgtgcg
    23521 ctcggcgatc tcgcgatcct cgtctcgctg tgggtctgcg ccgatcgagg ggtggatccg
    23581 atgttcggtg cgacgggcgc ggaagtccgg ccggtaacac ggtcacgttc gaagaagaac
    23641 ggccgcgcga atggtcgggc aaacggccgc gcgaacggca agcggcactg agccgccggg
    23701 ccgcgtcgtc agagcccgtt acagcccgtc gacgacggcg tcgtgaatgg cgcggcgcag
    23761 cgacttgatc gcttcggccg tcgtgccgtg acgcgaggga tgcacgcgat tcgtgagcag
    23821 cacgacccag cggcgatgcg cgatgtcgag ccagacggat gtgcccgtga agccggtgtg
    23881 accgacggcg cccgtgcgtg gccagcgatc gccggcctgc gaaactcccg gcgtgtgcga
    23941 cggcgcatcc caaccgaggc gccacggtga gccaggtgtc ggcgcatcgg tgaagaagcg
    24001 cgtgacgacg t
//
LOCUS       DATLBZ010000246         3606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3982072, whole genome shotgun sequence.
ACCESSION   DATLBZ010000246 DATLBZ010000000
VERSION     DATLBZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3982072"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="VMZ53_13070"
     CDS             complement(<1..2004)
                     /locus_tag="VMZ53_13070"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HUS29441.1"
                     /translation="MKRALSLLMTVALVPAAYAAPAKATKVRSVEGITEYKLANGLQV
                     LLYPDQTQSTVTVNITYLVGSRHEGYGETGMAHLLEHMMFKGSPRHRNVLKIVGERGG
                     QVNGTTWLDRTNYYETLPASQENLEWTLDLEADRMRNASISNDDLKTEFSVVRNEFEM
                     NENNPSSILDERLVSTAFLWHNYGKSTIGSRADIERVPTTALRTFYDKYYQPDNAVLV
                     VSGKFDEATALASVEKFFGPIAKPSRSLSATYTVEPVQDGERNVTLRRNGDVHVVALA
                     YHTVAGTSDDFPAVEAAVDVLTREPSGILYKKIVETKLASSVGGWDMATHDPYLAQFS
                     AQISDAKNVDKVEKIMQTEIEKLGAGKVDEKAVERWKVATLKEIELSMADSQAIAVEI
                     SEFAALGDWRTIFAYRDRVSKVTAADVQRIAKTFFKASNRTTGRFIPTKENDRAPLVE
                     TPDVAAYVKGIEGGTVKEQGEAFAATLDNIESRTKRVDLKGGIKAAFLPKKTRGGKVT
                     LQLQLHWGDEKSLKNTAIAASLLCSMMARGTAKKSYQDLQDAEDKLKAHIWCSSNADG
                     LVANIDTLRDNLPGAIDLATEMLKEPSFPAKEFEIVKQEQITSLEERKQDPMTVAFTT
                     LSQLTSKWPKGDPRYPMSTEEQIAAIKKTTLAEVKAFYKSFVGA"
     gene            complement(2130..>3606)
                     /locus_tag="VMZ53_13075"
     CDS             complement(2130..>3606)
                     /locus_tag="VMZ53_13075"
                     /inference="COORDINATES: protein motif:HMM:NF040596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MXAN_2562 family outer membrane beta-barrel
                     protein"
                     /protein_id="HUS29442.1"
                     /translation="SGLTARYQTAEQLCGAPSTIALMPSDITSTTDPDAGVDSLALGE
                     GLAKLDPAFLCGETTSATDRQLRIEGLKNGTPYTVVLLAIDKYGNAAGTYFTSRLTPV
                     PATDFWEDLQDQGSGVEGGFCLVAQAYGDDNPLTGAMRRFRDQTLASTAFGRALTKIY
                     YDSGVDLHGSVVLRAFTAIHLLPLIVVALLWHLFTLPGLLGLFALALISRRTSKKWRA
                     RMATAAALGTVLLLPTKAHAQQPYWEDETLGDTQDELAIGDPLRVKWHAGLRLGPYVP
                     GIDAQINMPHGKYEGPYEQMFGGYRLLPMLDIERFFWRGFGQAGIGLSIGYMSKSAKA
                     WQAGSDPNDPARPRSQGDENKFRLFPLSVSAVYRFTYLDDEFGVPLIPYARGGIGYYV
                     WWIVGPNGDFASACTDGGDTMGCAKTTAAGASLGLVGSIGLSIRAERIDESAARSMRE
                     SGIEHAGFYGEYSVGKVDGFGSDKKLSVGDATWFAGVDFEF"
BASE COUNT          562 a         1164 c         1185 g          695 t
ORIGIN      
        1 ggcgccgacg aagctcttgt agaacgcctt cacctcggcg agcgtcgtct tcttgatcgc
       61 ggcgatctgc tcctcggtcg acatcgggta gcgcggatcg cccttcggcc acttcgacgt
      121 cagctgcgac agcgtcgtga acgcgaccgt catcggatcc tgcttgcgct cctcgaggct
      181 cgtgatttgc tcctgcttca cgatctcgaa ttcctttgcc gggaagctcg gctccttcag
      241 catctcggtc gcgagatcga tcgcgccggg caggttgtcg cgcagcgtgt cgatgttcgc
      301 gacgaggccg tccgcgttgc tcgagcacca gatgtgcgcc ttgagcttgt cttcggcgtc
      361 ctgcagatct tggtagctct tcttcgcggt gccgcgcgcc atcatcgagc agaggaggct
      421 cgctgcaatc gcggtgttct tcagcgactt ctcgtcaccc cagtgcagct ggagctggag
      481 cgtgactttg ccgccgcgcg tcttcttcgg caggaatgcg gccttgatgc cgcccttgag
      541 gtcgacgcgc ttggtgcgcg attcgatgtt gtcgagcgtc gcggcaaacg cctcgccttg
      601 ctccttcacg gtgccgccct cgatgccctt cacgtaggca gcgacgtctg gcgtctcgac
      661 gagcggagcg cgatcgtttt ccttcgtcgg gatgaagcga cccgtcgtgc ggttgctcgc
      721 cttgaagaac gtcttcgcga tgcgctgcac gtctgcggcc gtgactttcg agacgcgatc
      781 gcgatacgcg aagatcgtgc gccagtcgcc gagcgcggcg aactccgaga tctcgacggc
      841 gatcgcttgg ctgtccgcca tcgacagctc gatctccttg agcgtggcga ccttccagcg
      901 ctcgactgcc ttctcgtcga ccttgcccgc gccgagcttc tcgatctcgg tctgcatgat
      961 cttctcgacc ttgtcgacgt tctttgcgtc gctgatctgc gccgagaact gcgcgagata
     1021 cggatcgtgc gtggccatgt cccagccacc gacgctcgac gcgagcttcg tctcgacgat
     1081 cttcttgtag aggatgccgc tcggctcgcg cgtcaggacg tcgaccgcgg cttcgacggc
     1141 cgggaagtcg tcagacgtgc cggcgaccgt gtgatacgcg agcgcgacca cgtgcacgtc
     1201 gccattgcgg cgcagcgtga cgttgcgctc gccgtcttgc acgggctcga cggtgtacgt
     1261 cgcggagagc gagcgcgatg gcttggcgat cggaccgaag aacttctcca cggacgcgag
     1321 cgccgtcgct tcatcgaact tgcccgagac gacgagcacc gcgttatcgg gctggtaata
     1381 cttgtcgtag aacgtgcgga gcgccgtcgt cggaacgcgc tcgatatctg cgcgcgagcc
     1441 gatcgtgctc ttgccgtagt tgtgccagag gaacgcggtc gacacgagcc gctcgtcgag
     1501 gatgctcgac gggttgttct cgttcatctc gaactcgttg cggacgaccg agaactccgt
     1561 cttgaggtca tcgttcgaga tcgacgcgtt gcgcatgcga tccgcttcga ggtcgagcgt
     1621 ccactcgagg ttctcctgcg acgccggcag cgtttcgtaa tagttcgtgc gatcgagcca
     1681 cgtcgtgccg ttcacctgac caccgcgctc accgacgatc ttcagcacgt tgcgatggcg
     1741 cggcgagccc ttgaacatca tgtgctcgag gaggtgcgcc atgccagtct cgccgtagcc
     1801 ctcgtgacga gagccgacga ggtacgtgat gttgacggtc acggtcgact gcgtctgatc
     1861 gggatagagc aggacttgca gaccgttcgc cagcttgtac tcggtgatgc cttcgaccga
     1921 gcggaccttc gttgccttcg ccggagccgc gtatgccgcg ggcactagcg ccaccgtcat
     1981 caatagagag agcgcacgtt tcatgggtag cctcgtagca cggacgccgt cggtgctgca
     2041 gcggttgcac gctgaccgca cctgttagca atgcgcagaa cgcgcagcgc ggcgcgccgg
     2101 ctgtcgcagg gtgtcgcggc cgggccgagt taaaactcga agtcgacgcc ggcgaaccag
     2161 gttgcgtcac cgacggagag cttcttgtcg gacccgaacc cgtcgacttt gccgaccgag
     2221 tactcgccgt agaaacctgc gtgctcgata ccactttcgc gcatcgaacg cgccgcgctc
     2281 tcgtcgatcc gttcggcgcg aatcgacagg ccgatcgacc cgacgaggcc aaggctcgcg
     2341 cctgccgcgg tcgtcttcgc gcaacccatc gtgtcgccgc cgtcggtgca cgccgatgcg
     2401 aagtcgccgt tcggcccgac gatccaccag acgtagtagc cgatgccgcc gcgggcatac
     2461 gggatgagcg gcacgccgaa ttcgtcgtcg agatacgtga agcgatacac ggcgctgacc
     2521 gacagcggga acaggcggaa cttgttctcg tcgccttgcg agcgcggccg agccggatcg
     2581 ttcggatcgc tgccggcctg ccacgccttc gccgacttgc tcatgtagcc gatcgagagg
     2641 ccgatgcctg cctggccaaa gccgcgccag aagaagcgct cgatatcgag catcggcagc
     2701 aagcgatagc cgccgaacat ctgctcgtac ggaccttcgt acttgccgtg cggcatgttg
     2761 atctgcgcgt cgatgccggg aacgtacgga ccgaggcgaa ggcctgcgtg ccacttcacg
     2821 cgcagcgggt cgccgatcgc gagctcgtct tgcgtgtcgc cgagcgtctc gtcttcccag
     2881 tacggctgct gcgcgtgcgc cttcgtcgga agaagcagca cggtgccgag cgcagcggcg
     2941 gtcgccatgc gcgcgcgcca cttcttgctc gtgcgtcgcg agatcagcgc gagtgcgaag
     3001 aggccgagca ggcccggcag cgtgaagagg tgccacagca gcgcgacgac gatgagcggc
     3061 aacagatgga tcgcggtgaa cgcgcggagc acgacgctgc cgtgcagatc gacgccgctg
     3121 tcgtagtaga tcttcgtgag cgcgcggcca aatgccgtcg acgcgagcgt ctgatcgcgg
     3181 aagcggcgca tcgcgcccgt cagcgggttg tcgtcgccgt acgcctgcgc gacgaggcag
     3241 aagccgcctt cgacgccaga gccttggtct tggaggtctt cccagaagtc ggtcgcggga
     3301 accggcgtga gcctcgacgt gaagtaggtg cccgccgcgt tgccgtactt gtcgatcgcg
     3361 aggagcacga ccgtgtacgg cgtgccgttc ttgaggccct cgatgcgtag ctgccggtcg
     3421 gtcgcgctcg tggtctcgcc gcagaggaac gcgggatcga gcttcgcgag cccttcgccg
     3481 agagcgagtg agtcgacgcc cgcatcggga tccgtcgtcg aggtgatgtc gctcggcatg
     3541 agagcgatcg tcgagggcgc gccgcacagc tgctcggccg tctggtagcg cgcggtcagg
     3601 ccgctc
//
LOCUS       DATLBZ010000247         7914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4731128, whole genome shotgun sequence.
ACCESSION   DATLBZ010000247 DATLBZ010000000
VERSION     DATLBZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4731128"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VMZ53_13080"
     CDS             complement(<1..1433)
                     /locus_tag="VMZ53_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS29443.1"
                     /translation="MGQILVVDDEQSMREFLAICLRRAGHEVTVAKSGSEALEKLRTK
                     PIDVVLTDLKMPGDLDGLGILKAIKTGAVRYTAQPGGTPSIIEPEVVLVTAYATADTA
                     LAAMKQGAYDYLTKPFQVEEINAVIARALEKRALVFENLALRDQVAGRARLANLLGKS
                     RAMQKVFELIGKIHSTKTSVLITGESGTGKELVARALHSEGSRAKTPFIAVNCGAIPE
                     ELMESELFGHKKGSFTGAVADHPGMFQEADGGSLFLDEIGELSPSLQVKLLRALQERK
                     VKPVGASDELEVDVRVIAATNRDLEAEVARGAFRADLYYRLNVIEIRIPPLRHRREDI
                     PLLAEHFLKRFGTEHGRALTITSEAMRKLESYDFPGNVRELENMIERAVALSSSNVIG
                     ASDLPEVKVPARQAAEVPADFPADGVDLDRLVADYERNWVLRALEQTGGIRKRAAVLL
                     GISFRSLRYRLEKLGIEKPDSGDDEDTG"
     gene            complement(1452..1979)
                     /locus_tag="VMZ53_13085"
     CDS             complement(1452..1979)
                     /locus_tag="VMZ53_13085"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS29444.1"
                     /translation="MLIPCERCKRHISTREDACPFCRHTSAFAAAALSVGMLLAGCDA
                     GNNKKPQGQGQGEGSAAAKRTYATLRGKVTDRNGRPLAGAQVSVQTANAVNGRSLMKS
                     VPADADGRYTLDLIEPGAYVLYFGYGNDSQQGSDQRAITVKADEDQTLDVSLDMRDVQ
                     QAMPYGAPPARRRVV"
     gene            complement(1995..2711)
                     /locus_tag="VMZ53_13090"
     CDS             complement(1995..2711)
                     /locus_tag="VMZ53_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS29445.1"
                     /translation="MSRLDNRAYYDDFAGWYERERHLPYHRMLDDLEVEIVERYARDK
                     QVLEVGCGTGLILHRVASFARDARGIDLSAGMLEKAEARGLAVAQASATELPIATASV
                     DVAYSFKVLAHIPDIQGAMREMARVVRPGGYVIAEFYNARSLRRLVKAIKPPSAVSEQ
                     TNDEHVYTRYDDADAIRSYLPPELEWVTTRGIRVITPVAAVLRVPLLGSAVRWAEHRL
                     ADAPVARDHGGFLVAICQRR"
     gene            2823..3311
                     /locus_tag="VMZ53_13095"
     CDS             2823..3311
                     /locus_tag="VMZ53_13095"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUS29446.1"
                     /translation="MRTQRGFTIIEIMIVVVIIGVLAAIAIPMFTGQMTKSKQSEALL
                     RLNELGKNAKVYYQANTTYPQGTAGVLPGADGDACKGPGRKFAADSAAWQGDQVWLAL
                     DFHVDEANLFSYHYTSTTANNAEALAVGDTDCDKHLVTYKLALSVPENPAGEIIKPTS
                     DD"
     gene            complement(3325..4362)
                     /locus_tag="VMZ53_13100"
     CDS             complement(3325..4362)
                     /locus_tag="VMZ53_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HUS29447.1"
                     /translation="MGIISFIKGGVAELAIARPDAAKDFWVYKHPDQTIPMKAQLTVD
                     SDEVALFFKDGKYVGSFSAGRHTMDASNIPFLGQLVDKFTGGNVFICEVYFVNTREMP
                     SLKFGTSIGDVMDPQTKLRVRIMVYGEFSAKVIDPVKFVIGLVGQRATTNDAFLGWFK
                     SQVQKTMKDGIAELIVKQDWPLIKVTSGAFTEEIEATTLQNVRKNIEPYGVEIIRFGD
                     FSISMDQADRERLDKLVERMAYISASGGMAGYQQLAQAEMMMGAAEGMKKGGGAGTGL
                     EGAGIGMGFAMANQMGGMMMGNQQRPGGGGGESKDQIMGMLKQLGELKAAGVLTDAEF
                     EAKKKELLAKL"
     gene            4508..5863
                     /locus_tag="VMZ53_13105"
     CDS             4508..5863
                     /locus_tag="VMZ53_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29448.1"
                     /translation="MTAFVKKALLLVAFVAACGPGDRGDDGNGSGSGSGSNGCADCVT
                     VTGKVYAPKWAPGQVPAGQEIPVFGATIYVTDAKPAPIPQQTYCESCADTPQGSVASK
                     HDGSFELQVTGPGHYWLIIEKGSFRLEQELDLTFGTTTLPPTATTLPDRQDPAHGAWI
                     PKVAVVRGNYDAVEDILGKIGFGTMSGNKMTNPDGEITFYEWGGTGNTSADYLISNIS
                     ELRKYHIVFFPCSTLVNDTATADQAKLKNIRQFVADGGKLYVTDWSGEVGDRAFPPQI
                     KLGSDGFGTPDTVGTYNPQTLDGTITTPGDADGSLYNSNDGKAIDPDLNMWLGLQTGP
                     TEENPVPTLYNANSFPVNDNWNWIDKLNSVMKGVDENNMPVYDDPKAWVTASDESGHG
                     PKPVAVTYEPAGCGKVLYSTFQTAGASASTSHAGLTPQERVLLFLIMEISDCTQNPVI
                     L"
     gene            5877..6905
                     /locus_tag="VMZ53_13110"
     CDS             5877..6905
                     /locus_tag="VMZ53_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HUS29449.1"
                     /translation="MLTPARLIGLLVVLNVALVIVGAVAVRAPTKAKGAGIRVGLVFD
                     VGGKNDKSFNEAAYRGLERARTELGVRVQYIEPSEGADRESAMRQLAAQNVDLVIGVG
                     FIFGPDLERLSKQFPNVKFAGIDYSPSPGVGDLPNLAGLRFREHEGSFLVGAIAGLTT
                     HSRIVGFVGGMKIPLIRKFEAGYEAGVKHVCPSCRVLSAYAGSEPKAFADPPLGQELA
                     AAQYGQGADIIFHAAGKTGDGVFAAARQRKARAIGVDSDQFESAPCCIVTSMVKRVDV
                     AVVDVIKDVIEHRFQGGLHELGLAEHGVTFVADERNQGRLLPLDVVQKVNVLREEIIA
                     GKIVVPSQ"
     gene            6902..>7914
                     /locus_tag="VMZ53_13115"
     CDS             6902..>7914
                     /locus_tag="VMZ53_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS29450.1"
                     /translation="MSAASNEAAALLLDATLTKRFGAREVLQDATLRVRPGTVHALVG
                     ENGAGKSTLVKIVAGVLRGDRGTLTLHGAPRPVQTWTRQHARAAGIGIVQQHGAFAGT
                     LSIVENAVLGVEPFRGPLLDLEPTATKLRDLATKVGLPIDPHAPAGALSLGAAQRAEI
                     VTALHHGARLLILDEPTAVLAPIEVEGLLERLRSLAASGTTIVIVTHKLDEVRAVADD
                     VTVLRAGKTVATFDVHDAADAADAADAADAADAASDAPYRKLDVQAVAKAMVGADLAE
                     PTTLAAPAADAKLAFHCADLGVAADLQNISLQVRNGERVGVAGVDGNGQRELALAIAG
                     LVP"
BASE COUNT         1355 a         2594 c         2578 g         1387 t
ORIGIN      
        1 ccggtgtcct cgtcgtcgcc ggaatcgggc ttctcgatgc cgagcttctc gaggcgatag
       61 cgcagcgagc ggaagctgat gccgaggagc acagcggcgc gcttgcgaat cccgccggtc
      121 tgctcgagcg cgcgcagcac ccagttgcgc tcgtagtccg cgaccagacg atcgaggtcg
      181 acgccatccg ccggaaagtc cgcgggcact tcggcggctt gccgcgccgg cactttgacc
      241 tcgggcaaat cgctcgcgcc gatcacgttg ctcgatgaca gcgcgaccgc gcgctcgatc
      301 atgttttcga gctcgcgcac gttgccgggg aagtcgtagc tctcgagctt gcgcatcgct
      361 tcgctcgtga tcgtgagcgc gcgaccgtgc tcggtgccga accgcttgag gaagtgctcg
      421 gcgagcagcg ggatgtcttc gcggcgatgg cgcagcggtg ggatgcggat ctcgatcacg
      481 ttgaggcggt agtagaggtc tgcgcgaaac gcaccgcgcg cgacttccgc ttcgagatcg
      541 cgattcgtcg cagcgatgac gcgcacgtcg acttcgagct cgtccgacgc gccgaccggc
      601 ttcactttgc gctcctgcag cgcgcgcaac agcttcacct gcagcgacgg cgacagctca
      661 ccaatctcgt cgaggaacag cgagccgccg tcggcttctt ggaacatgcc ggggtggtcg
      721 gcgacggcgc ccgtgaacga gcccttcttg tgaccgaaca gctccgactc catgagctct
      781 tcgggaatcg cgccgcagtt gaccgcgatg aacggcgtct tcgcgcgaga gccttcgctg
      841 tgcagtgcgc gcgcgacgag ctctttgccc gtgccgctct cgcccgtgat gagcacgctc
      901 gtcttcgtcg agtggatctt gccgatcagc tcgaagacct tctgcatcgc gcgcgacttg
      961 ccgagcagat tggcgaggcg cgcacggccg gcgacttgat cgcgaagcgc gaggttctcg
     1021 aagacgaggg cgcgtttctc gagcgcgcgc gcgatgacgg cgttgatctc ttcgacctgg
     1081 aacggcttcg tcaggtagtc ataggcgcct tgcttcatcg cggcgagcgc ggtgtcggcg
     1141 gtcgcgtatg cggtgacgag cacgacttcc ggctcgatga tcgatggcgt gccgccgggc
     1201 tgcgcggtgt aacgcacggc gccggtcttg atcgctttca ggatgccgag gccgtcgagg
     1261 tcgccgggca tcttgaggtc ggtgagcacc acgtcgatcg gcttggtgcg cagcttctcg
     1321 agtgcttcgc tgccgctctt ggcgaccgtg acctcgtggc ccgcacgacg cagacagatc
     1381 gcgaggaact cgcgcatcga ctgctcgtcg tcgacgacca ggatctggcc cacgtgctga
     1441 ctatacctgg tctagaccac gcgacgacgc gccggtgggg ctccgtaggg cattgcctgt
     1501 tgcacgtcgc gcatgtcgag ggagacatcg agtgtctggt cctcgtccgc cttcacggtg
     1561 atcgcccgct gatcgctgcc ttgctgcgaa tcgtttccgt agccgaagta gaggacgtag
     1621 gcgccaggct cgatcaggtc gagcgtgtag cgtccgtccg cgtcggcagg caccgacttc
     1681 atgagcgagc gcccgttcac cgcgtttgcg gtttggaccg agacctgcgc gccggccaat
     1741 gggcgaccgt tccggtcggt gaccttgccg cgaagcgttg cataggtgcg cttcgccgcg
     1801 gccgagcctt cgccctgtcc ttggccctgc ggcttcttgt tgtttcccgc atcgcatccg
     1861 gcgaggagca tgccgaccga tagcgctgct gctgcaaacg cactcgtgtg ccggcagaac
     1921 ggacaagcat cttcacgcgt ggagatgtgt cgcttgcagc gctcgcatgg gatcagcatc
     1981 cgctgatcat aacgctaacg gcgctggcag atcgcaacga gaaatccgcc gtggtcgcgc
     2041 gcgacgggtg cgtcggcgag gcggtgctcc gcccagcgca cggcagaacc gagcagcggg
     2101 acgcgcagca cggcggcgac cggcgtgatc acgcgaatgc ctcgcgtcgt gacccactcg
     2161 agctccggcg ggagatagct gcgaatcgca tcggcgtcgt cgtagcgcgt gtacacgtgc
     2221 tcgtcgttcg tctgctcgga gacagcgctc ggcggcttga tcgctttgac cagacgacgc
     2281 agcgatcgcg cgttgtagaa ctcggcgatc acgtagccgc cggggcgaac gacgcgcgcc
     2341 atctcccgca tcgcgccttg gatgtccggg atgtgagcga gcactttgaa cgagtacgca
     2401 acgtcgaccg aggcggtcgc aattggcagc tcggtggcgc tcgcttgcgc gacggcgagc
     2461 ccgcgcgctt ctgctttctc gagcattccg gccgagagat cgatgcctcg cgcatcgcga
     2521 gcaaacgacg cgacacgatg aaggatgagg cccgtgccgc aacccacctc caggacctgc
     2581 ttgtcccggg cgtacctctc gacgatttcg acctccaggt cgtcgagcat gcggtggtag
     2641 ggcaggtggc gctcgcgctc gtaccagccg gcgaagtcgt cgtaatacgc gcggttgtcc
     2701 aagcgagaca tcggtaagtg cttactacag cggtgcgata aacgctcagg atcgccagcc
     2761 ttacctcact ggtacctgaa tgaaaatccc cctcggtcga cccctatgtc agggtgatca
     2821 gtatgaggac gcaacgggga ttcacgatca tcgagatcat gatcgtcgtc gtgatcatcg
     2881 gcgtcctagc cgcgatcgcg attccgatgt tcacgggaca aatgacgaag tccaagcagt
     2941 cggaggcgct gctccgactc aacgagctcg gaaaaaacgc gaaggtttac tatcaagcca
     3001 acacgacgta cccgcagggg acggccggcg ttctccccgg cgccgacggt gacgcgtgca
     3061 agggaccggg gcgcaagttt gccgccgaca gcgcggcgtg gcagggcgat caggtgtggc
     3121 tcgcgctcga cttccacgtc gacgaggcga atctgttctc gtatcactac acgtcgacca
     3181 cggcgaacaa cgccgaggcg ctagccgttg gcgatacgga ttgcgacaag cacctcgtca
     3241 cgtacaagct cgcgctgtcc gtgccggaga atccggcggg cgagatcatc aagcccacaa
     3301 gcgacgacta ggccgcgagc ggtcctagag cttcgcgagc agctctttct tcttcgcttc
     3361 gaactcggca tcggtgagga cgccggcggc cttcagctca ccgagctgct tgagcatgcc
     3421 catgatctgg tccttgctct cgccaccgcc gccacctggg cgctgctggt tgcccatcat
     3481 catgccgccc atttggttcg ccatcgcgaa gcccatgccg atgcccgcgc cttcgaggcc
     3541 ggtgccggca ccgccgccct tcttcatgcc ttcggccgcg cccatcatca tctcggcttg
     3601 cgcgagctgc tgatagcccg ccatgccgcc cgacgcgctg atgtacgcca tgcgctcgac
     3661 gagcttgtcg aggcgctcgc ggtcggcctg atccatcgag atcgagaagt cgccgaagcg
     3721 gatgatctcg acgccgtacg gctcgatgtt cttgcgaacg ttctgcagcg tcgtcgcttc
     3781 gatctcttcg gtgaacgcgc ccgaggtgac tttgatcagc ggccagtctt gtttgacgat
     3841 cagctcggcg atgccgtcct tcatcgtctt ttggacttgg ctcttgaacc agccgaggaa
     3901 cgcgtcgttc gtcgtggcgc gctggccgac gagaccgatc acgaacttca ccgggtcgat
     3961 gactttggcg gagaactcgc cgtagaccat gatgcggacg cgcagcttcg tctgcggatc
     4021 catcacgtcg ccgatcgagg tgccgaactt gagcgacggc atctcgcgcg tgttgacgaa
     4081 gtagacctcg cagatgaaca cgttgccgcc cgtgaacttg tcgacgagct ggccgaggaa
     4141 cgggatgttc gacgcgtcca tcgtgtgacg gccggcggag aagctgccga cgtacttgcc
     4201 gtctttgaag aagagcgcga cctcgtcgga atcgacggtc agctgcgcct tcatcgggat
     4261 cgtttggtcg gggtgcttgt agacccagaa gtctttcgcg gcgtccggac gcgcgatcgc
     4321 aagctcggca actccaccct tgatgaacga gatgattccc atcgtttgct ctcctgcgca
     4381 gggccgtttg cagtccagtg gcccggctgt aacctagcac acagcctaac cacgatcggc
     4441 gcgaacagaa gtcctcggtc acgttttcac ttgcacggcg aaggtagcga acgtgtaatg
     4501 cgcgccgatg acggcctttg tgaagaaagc tcttctgctc gtcgcattcg tcgctgcgtg
     4561 cggtcctggt gatcgcggcg acgacgggaa tggaagtggc agcggcagcg gttccaacgg
     4621 gtgcgctgac tgcgtcacgg tgacgggcaa ggtctacgcg ccgaagtggg caccgggcca
     4681 ggttcctgcg ggtcaggaga ttccggtgtt cggtgcgacg atctacgtga ccgacgcaaa
     4741 gccggcgccg attccgcagc agacgtactg cgagtcgtgc gcggacacgc cgcaaggcag
     4801 cgtcgcttcg aagcacgatg gctcgttcga gctgcaggtg acgggccccg gtcattactg
     4861 gctcatcatc gagaagggct cgttccgcct cgagcaggag ctcgacttga cgttcggcac
     4921 gacgacgctt ccgccgacgg ctacgacgct tcccgatcgt caggacccgg cgcacggcgc
     4981 atggattccg aaggtcgcgg tcgttcgcgg caactacgac gcggtggaag acatcctcgg
     5041 caagatcggc ttcggcacga tgtcggggaa caagatgacg aaccccgatg gcgagatcac
     5101 gttctacgag tggggcggca cgggcaacac gagcgccgac tacctcatct cgaacatcag
     5161 cgagctgcgc aagtatcaca tcgtgttctt cccgtgctcg acgctcgtga atgacacggc
     5221 gacggcggat caggcgaagc tgaaaaacat ccgtcagttc gtcgccgatg gcggcaagct
     5281 gtacgtgacg gactggtcgg gtgaagtcgg cgatcgcgcg ttcccgccgc agatcaagct
     5341 cggcagcgat ggcttcggta cgccggacac cgtgggcacg tacaacccgc agacgctcga
     5401 tggcacgatc acgacaccgg gcgacgcgga cggcagcctc tacaactcca acgacggcaa
     5461 agcgatcgat ccagacctca acatgtggct cggcctgcag acgggaccca ccgaggagaa
     5521 tccggtcccg acgctctaca acgcgaactc gttccccgtg aacgacaact ggaactggat
     5581 cgacaagctg aactccgtga tgaagggcgt cgacgagaac aacatgcccg tctacgacga
     5641 cccgaaggcg tgggtgacgg cgtcggatga atcgggtcac ggcccgaagc cggtcgcggt
     5701 gacgtacgag cccgccggtt gcggcaaggt tctctactcg acgttccaga cggcaggcgc
     5761 atcggccagc acgtcacacg ccggcctgac tccgcaggag cgcgtgctgc tgttcctgat
     5821 catggagatc agcgactgca cgcagaaccc cgtcattctc tagtactgtc cggcgggtgc
     5881 tcacgcccgc gcgcttgatc gggctgctcg tcgtactgaa cgtcgcgctg gtcatcgtcg
     5941 gcgcggtggc ggttcgcgcg cccacaaagg cgaagggcgc tggcattcgc gtcggcctcg
     6001 tgttcgacgt cggcggcaag aacgacaagt cgttcaacga ggcggcgtat cgcggtctcg
     6061 agcgcgcgcg taccgagctg ggcgtgcggg tgcagtacat cgagccgtcc gagggcgcag
     6121 atcgcgagag cgcgatgcgg cagctcgctg cgcagaacgt cgacctcgtg atcggcgtcg
     6181 gcttcatctt tggtccggat ctcgagcggc tctccaagca gttcccgaac gtgaagttcg
     6241 cgggcatcga ttactcgcct tcgcccggcg tcggcgacct gccgaacctc gcgggcctgc
     6301 gctttcgcga gcacgaaggc agctttctcg tcggcgcgat cgcgggactc acgacccact
     6361 cgcggatcgt cggcttcgtg ggcggcatga agatcccact gattcgcaag ttcgaagcag
     6421 gctacgaggc gggcgtgaag cacgtgtgcc cgtcgtgtcg cgtgctctcg gcctacgcgg
     6481 gcagcgagcc aaaagcgttc gccgatccgc cgctcggtca ggagctcgcc gccgcgcagt
     6541 acgggcaggg tgccgacatc atctttcacg ccgcgggcaa gacaggtgac ggcgtgttcg
     6601 cagccgcgcg ccagcgcaag gcccgcgcaa tcggcgtcga cagcgatcag ttcgagtccg
     6661 cgccgtgctg catcgtgacg agcatggtga agcgcgtcga tgtcgccgtg gtcgacgtca
     6721 tcaaggacgt gatcgagcat cgcttccaag gcggcctgca cgagctcggc ctcgcagagc
     6781 atggcgtgac gttcgtcgcc gacgagcgca accaaggtcg cctgcttccg ctcgacgtcg
     6841 tgcagaaggt caacgtgctg cgcgaagaga tcatcgcggg caagatcgtg gtgccgtcgc
     6901 aatgagcgcc gcatcgaacg aggcggcagc gctgttactc gacgcgacgc tcacaaagcg
     6961 gttcggcgcg cgcgaagtgc tgcaagacgc gacgctccgc gttcgtccgg gaaccgtaca
     7021 cgcgctcgtc ggcgaaaacg gcgcaggcaa gagcacgctc gtgaagatcg tcgccggtgt
     7081 cctgcgcggc gatcgcggaa cgcttacgct gcacggtgcg ccgcgccccg tccagacatg
     7141 gacgcgccag cacgcgcgcg ccgccggcat cggcatcgtc cagcagcacg gcgcattcgc
     7201 gggcacgctg tcgatcgtcg aaaacgccgt cctcggtgtc gagccattcc gcggaccgct
     7261 gctcgatctc gagccgacgg ccaccaagct tcgcgacctc gcgacgaaag tcggactgcc
     7321 gatcgatccg cacgcgcctg ccggcgccct ttcgctcggc gccgcacagc gcgccgaaat
     7381 cgtcacggcc ctccaccacg gcgcacgcct tcttatcctc gacgagccga ccgcggtcct
     7441 cgctccgatt gaagtcgaag gcctgctcga gcgccttcgc tcgctcgccg cgtccggcac
     7501 gacgatcgtg atcgtcacgc acaagctcga cgaagtgcgc gccgtcgccg acgacgtcac
     7561 ggtcctccgc gcaggcaaga ccgtcgcgac gttcgacgtg cacgacgccg ccgacgccgc
     7621 cgacgccgcc gacgccgccg acgccgccga cgccgcgagt gacgctccat atcgcaagct
     7681 cgacgtccaa gccgttgcca aagcgatggt cggcgcggac ctcgccgaac cgacgacgct
     7741 cgctgcgccc gccgccgacg cgaagctcgc gttccactgc gcggacctcg gcgtcgcggc
     7801 cgacctccag aacatctcgc tgcaggtgcg caatggcgag cgcgtcggtg tcgccggcgt
     7861 cgacggcaac ggccagcgcg agctcgctct cgccatcgcg ggcctcgtcc cgca
//
LOCUS       DATLBZ010000248         5324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8329739, whole genome shotgun sequence.
ACCESSION   DATLBZ010000248 DATLBZ010000000
VERSION     DATLBZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8329739"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..632
                     /locus_tag="VMZ53_13120"
     CDS             117..632
                     /locus_tag="VMZ53_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HUS29451.1"
                     /translation="MTATVRTFGGKTPQLGAGVFLAETCSVIGDVVIGDESSIWYSTV
                     VRGDVMPIRIGARTSIQDGAVIHVTSEFSGTIIGDDCTVGHSAIIHACTVEDFCLIGM
                     GSIILDGARIGRGSLVGAGALVTPGTDIPPGSLVIGSPAKVKRPLNEKERTQIAYGAQ
                     HYVELARRYRE"
     gene            723..1583
                     /locus_tag="VMZ53_13125"
     CDS             723..1583
                     /locus_tag="VMZ53_13125"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS29452.1"
                     /translation="MRSSIVLLITLVLSMRVAHADSVDTLVKQLGDSSKNVRLAAALN
                     LAKLADQKAVLPLAKTVTSDDDASVRAAAAVALGKTVNSSTKESVKKLAIANLKAAAN
                     DSSSTVKAQAQKALETITGASSSSGGTNTGTASGSGKGGIYVNIGPMSSKTSSSKNDK
                     LRALMVKTTEKTLAKVASSMQTTWAGGAPSKSTLASKGVAGFYVDGTLNTLDIKTSGG
                     GATISCKVSMLLADFPDKSVFGFLNGGASVTASTSQSDQDLASEDCVTAVIENLIANK
                     IVPTIKSKVP"
     gene            1585..2730
                     /locus_tag="VMZ53_13130"
     CDS             1585..2730
                     /locus_tag="VMZ53_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29453.1"
                     /translation="MADDAVPKPMPRANTGAYKRSWKNLLLNKRYQLRFTLFMVGVSA
                     VLMIVLGWWVMKEANEATTVAMARVRGEACPKVPEVIEVPGLTQEESVPMNIEEAPPT
                     DTPPPAQDKPPVEEKLDPQAPKEAKPEPSKVDPAHVAANNGQNDILAVKALWCTDAEC
                     KPEAAMPLQVKVKGCDEYVKQKLANADAVDALRTAQIPVVKCEGGGEPFSVADAEPER
                     RATVQLEESSMTITPTLPTDYADRIVAHYTCEMRQVGSIRALEAGRMRILYVLLATGL
                     LLMVGLAMYGIKMTHKVAGPLFKVSLYLAKMRDGRLDKVWNLRKGDQLVDFYDHFKSA
                     HAGIVQLEKDDIAKLKDTIAAAEAAGIDDEGLDVLRRTLARKEKSIE"
     gene            2750..3412
                     /locus_tag="VMZ53_13135"
     CDS             2750..3412
                     /locus_tag="VMZ53_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29454.1"
                     /translation="MKDGKLGGDVPKGGGPGKPKTVYKRRLSNYLLDKRLQLRYVLLV
                     TILSGVISGSLGYLIYQQRHAASESIERDLAALTQDDQSLKDFQHQVAAGMAAEDRAL
                     VYKMVGVGIGLVVILSLYLLIMTHKVAGPLYKVSGYFDKMAAGRVGQVWGLRKGDMLQ
                     DFFANFKEMHDAVRGRLQVDVTNMETASGVLRARAAGNEKLIAALDEMDKHIAARKQA
                     LA"
     gene            3435..5291
                     /locus_tag="VMZ53_13140"
     CDS             3435..5291
                     /locus_tag="VMZ53_13140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS29455.1"
                     /translation="MGNALLELGDRYWALGLPAAAKSALQRALAITDDVAPALRLTTI
                     ALAQGDSAAARAFANEAAKRSPGPDTKILVGRAQLAAGEVAAARMSLSAALDSPKLKP
                     WERARAHLELSRAADAQSDTQGAAAQAGAAFEAVVTAAQKGPPELAIIEEVAAAVVAH
                     GRGADAMQTLDTVRGTTGSQLCIALLLTARQAASDSGVTDTMIDDALAAAEEESPSSA
                     LRLRRLERRARKLSQSERDQLIGELETLAINVAAEDLAAYDRARLHFLRASLCEEDPN
                     TRERAEAAYRKGLTFQPGHTAAACRLALLVLDRGDQAGALAEIERALRIDSSHGMAWR
                     NAARMLDAQSPSLGVVVGRLLDAANPGAGSAAGGVAPRLVTATAEVARHDVLAGVYAH
                     GHRVKNLLGIIGARTRSARKLAGEHEVGDRLKELEKDVTALYEEWAQYLRSMQAPTPT
                     VVVVPLSPLLHEVVIAAQARTQNVSILLELTAALPDIRGDRMLLREALLNVISNAAEA
                     CGPTNGDVSVRVRSIAGPAASAAPIIEIVVADTGPGIPRAHLGRLFVPGFTTKETGSG
                     VGLAIAERVVSAHHGRITVDSEEGKGTTITITLPTDKHGLAVLPRWTSAERG"
BASE COUNT         1003 a         1709 c         1792 g          820 t
ORIGIN      
        1 gagagggacg ccctttcccg tatctgctcg tggtttgtgc ggtcctttcg cagcagcgaa
       61 agatcaggac gggtgctcgc ttacgcggac gcttacgcct acgcttacga acgccgatga
      121 ccgcaaccgt tcggacgttc ggcggcaaga cgcctcagct cggcgcaggc gtgtttctcg
      181 ccgagacctg ctcggtgatc ggcgacgtcg tcatcggcga cgagagctcg atctggtaca
      241 gcaccgtcgt gcgcggggac gtcatgccga ttcgcatcgg cgcgcgcacg tcgatccagg
      301 atggcgccgt cattcacgtg acgagcgagt tctccggcac gatcatcggc gacgactgca
      361 ccgtcggtca ctccgcgatc attcacgcgt gcacggtcga agatttctgc ctcatcggca
      421 tgggctccat catcttggac ggcgcgcgca tcggtcgcgg cagcctcgtg ggtgcaggtg
      481 cgctcgtgac gccaggcact gacatccctc ccgggagtct cgtgattggc tcgcctgcaa
      541 aggtgaaacg gccactgaat gagaaggagc gaacgcagat cgcgtacggc gcgcagcact
      601 acgtcgagct cgcacgtcgc tatcgcgagt gatcttcgtc ggcggtcgct tgcacgagtg
      661 gggatcgact cgttactctg aatcgagtgg ccgggggctc cgtctcatca ggcacaccgc
      721 gcatgcgctc gagcatcgtc ctcttgatca cgttggtgtt gtcgatgcgc gtcgcgcatg
      781 ccgatagcgt cgacacgctc gtcaagcagc ttggcgattc gtcgaagaac gttcggctcg
      841 ccgcggcgct gaacctcgca aagctcgccg atcagaaggc ggtcctgccg ctcgcgaaga
      901 ccgttacgtc ggatgacgac gcgagcgtcc gcgcagcggc agcagtcgcg ctcggcaaga
      961 ccgtgaactc gagcacgaag gagtcggtca agaagctcgc gatcgcgaac ctcaaggccg
     1021 ctgcaaacga ctcgagctcg acggtgaaag cgcaggcgca aaaagcgctc gagacgatca
     1081 ccggagcgag cagcagctcg ggcggcacga ataccggcac cgcgagcggc agcggcaagg
     1141 gcggcatcta cgtgaacatc gggccgatgt cgtcgaagac gagcagcagt aagaacgaca
     1201 agctccgcgc gttgatggtg aagacaacgg agaagaccct cgccaaggtc gcgagcagca
     1261 tgcagacgac gtgggcgggc ggcgcgccca gcaaatcgac cctggcctcc aagggtgtcg
     1321 cgggcttcta cgtcgacgga acgctcaaca cgctggacat caaaacttcg ggtggcggcg
     1381 cgacgatctc gtgtaaagtc agcatgttgc tcgcagactt tcccgataag agcgtgtttg
     1441 gcttcttgaa tggcggcgcg tccgtcacgg ccagcacgtc gcagagtgac caggacctcg
     1501 cgagcgagga ttgtgtcacc gcagtgatag agaacttgat cgcgaacaag atcgtaccga
     1561 cgatcaagtc gaaggttccc tgagatggcg gacgacgcgg ttccaaagcc aatgccccga
     1621 gccaacacgg gtgcgtacaa gcgcagctgg aagaacctcc tgctcaacaa gaggtaccag
     1681 ctccgcttca cgctcttcat ggtgggcgtc tcggccgtgc tgatgatcgt cctcggttgg
     1741 tgggtcatga aggaggccaa cgaggcgacg accgtcgcga tggcccgcgt gcgcggcgaa
     1801 gcgtgtccga aggtgcccga agtgatcgag gtgcccggcc tgacgcaaga agagtccgtg
     1861 ccgatgaaca tcgaggaggc accgccgacg gatacgccgc ctcctgcgca ggacaagccg
     1921 cccgtcgagg agaagctgga tcctcaagcg cccaaagagg cgaagcccga accgtcgaag
     1981 gtcgatccgg cgcacgtcgc cgcgaacaac gggcagaacg acatcctcgc cgtgaaggcg
     2041 ctgtggtgca ccgacgccga gtgcaagccc gaagcggcca tgccgctgca ggtgaaggtc
     2101 aaaggctgcg acgagtacgt caaacagaag ctcgcgaacg cagacgcggt cgacgcgctg
     2161 cgcaccgcac agattcccgt cgtgaagtgc gaaggcggcg gggagccgtt cagcgtcgcg
     2221 gacgccgagc ccgagcgccg cgcgacggtg cagctcgaag agtcgtcgat gacgatcacg
     2281 ccgacgctgc cgaccgatta cgcggaccgc atcgtcgcgc actacacctg cgagatgcgc
     2341 caggtcggct cgatcagagc gctcgaagcc ggccgcatgc gcattctcta cgtgctgctc
     2401 gcgaccggtc tgctgctcat ggtcggcctc gcgatgtacg gcatcaagat gacccacaag
     2461 gtcgccggcc ctctgttcaa ggtgagcctc tacctcgcga agatgcgcga cggccgcctc
     2521 gacaaggtgt ggaacctccg caagggcgac cagctcgtcg atttctacga ccacttcaag
     2581 agcgcgcacg ccggcatcgt gcagctcgag aaggacgaca tcgcgaagct caaagacacg
     2641 atcgctgccg ccgaagccgc cggcatcgac gacgaaggcc tcgacgtgct tcgcaggacg
     2701 ctcgcgcgca aggagaagtc cattgagtga cgtaccctcc gcaaccgtcg tgaaggacgg
     2761 caagctcggt ggcgacgtgc cgaaaggcgg cggccccggg aagcccaaga cggtctacaa
     2821 gcgccgcctg tcgaactacc tgctcgacaa gcgattgcag cttcgttacg tgctgctcgt
     2881 aacgatcctg tccggcgtga tctccggatc gctcggctac ctcatctatc agcagcggca
     2941 cgccgcatcg gaatcgatcg agcgcgacct cgctgcactc acgcaagacg atcagtcgct
     3001 caaggacttc cagcaccagg tcgccgcggg catggctgcc gaagatcgcg cgctcgtcta
     3061 caagatggtc ggcgtcggca tcggcctcgt cgtcatcctg tcgctgtact tgctcatcat
     3121 gacccacaag gtcgctggcc cgctctacaa ggtcagcggc tacttcgaca agatggcggc
     3181 gggacgcgtc gggcaggtct ggggcttacg caaaggcgac atgctccaag acttctttgc
     3241 gaacttcaaa gaaatgcacg acgccgtacg agggcgattg caggtcgacg tgacgaacat
     3301 ggagaccgcc tcgggcgtgc tgcgcgcgag ggccgccggc aacgagaagc tgatcgctgc
     3361 gctcgacgag atggacaaac acatcgcggc gcgcaagcaa gcgctcgcgt aagtcgctgg
     3421 agtaacatcg cggcgtgggc aacgcgctgc tcgagctcgg tgatcgctac tgggcgctcg
     3481 gtctgccggc agccgcgaag agcgcgctcc agcgtgcgct ggcgatcacg gatgatgtcg
     3541 cgccagcgct gcgcctgacg acgatcgcgc tcgcgcaggg cgattcggct gcggcgcgtg
     3601 cgtttgcgaa cgaggcggcg aagcgatcgc ccggccccga tacaaagatt ctcgtcggtc
     3661 gcgctcagct cgcagcggga gaagtcgcgg cggcgcggat gtcgctgtcg gccgcgctcg
     3721 attcgccgaa gctgaagccg tgggagcggg cgagggcgca tctcgagctg tcgcgcgccg
     3781 ccgatgcgca gagcgatacg caaggtgcag cggcgcaagc aggcgcagcg ttcgaggcgg
     3841 tcgtgacggc agcgcagaag gggccgcccg agctcgcgat catcgaggag gtcgcagcgg
     3901 cggtcgtcgc gcacggccgc ggtgccgatg cgatgcagac gctcgacacc gtgcgcggaa
     3961 cgacggggag ccagctctgc atcgcactgc tgctgaccgc gcggcaagca gcgagtgaca
     4021 gcggtgtgac ggacacgatg atcgacgacg cgctcgcggc ggcggaggaa gaatctccgt
     4081 cgagcgcgct gcgccttcgc cggctcgagc gacgcgcgcg caagttgtcg cagagcgaac
     4141 gcgatcaatt gatcggcgag ctcgagacct tggcgatcaa cgtcgcggcg gaggacctcg
     4201 ctgcgtacga ccgcgcgcgg ctgcacttct tgcgcgcgag cctgtgcgaa gaagatccga
     4261 acacacgcga gcgtgcagaa gcggcctatc gcaaaggcct cacgttccag cccggccaca
     4321 ccgcagcggc gtgccgtctc gctctgctcg tgctcgatcg cggcgatcaa gcgggcgctc
     4381 tcgccgagat cgaacgcgcg ctgcgcatcg actcgagtca cggcatggcg tggcgcaacg
     4441 cagctcgcat gctcgacgcg cagtcgccgt cgctcggcgt cgtcgtcggc cggttgctcg
     4501 acgcggcgaa tcccggtgcg ggctccgcgg caggtggcgt cgcaccgagg ctcgttaccg
     4561 cgacggcgga ggtcgcgcgc cacgacgtgc tcgcgggcgt ctatgcgcac ggccatcgcg
     4621 tgaagaatct gctcggcatc atcggcgccc gcacgcgcag cgcacgcaag ctcgccggcg
     4681 agcacgaggt cggcgatcgc ttgaaggagc tggagaagga cgtcacggcg ctctacgaag
     4741 aatgggcgca gtacctccgt tcgatgcagg cgccgacgcc gaccgtcgtt gtcgtgccgc
     4801 tgtcgccgct tctgcacgag gtcgtcatcg ccgcacaggc acgcacgcag aacgtgtcga
     4861 tcctcctcga gctgacggcc gcgcttcccg acattcgtgg cgatcgcatg ctgctgcgcg
     4921 aggcgctgct caacgtgatc agcaacgcgg ctgaagcgtg tggcccgacg aacggtgacg
     4981 tctccgtccg cgttcgctcg atcgcaggcc ccgccgcgag tgcggcaccg atcatcgaga
     5041 tcgtcgtcgc ggataccggc ccgggaatcc cgcgcgcgca cctcggccgc ttgttcgtac
     5101 cgggcttcac gacgaaggag accggctcgg gcgttggcct cgcgatcgcg gagcgcgtgg
     5161 tgtctgcgca tcacgggcgc atcaccgtcg attcggaaga gggcaagggc acgacgatca
     5221 cgatcacgct gccgaccgac aagcacggcc tcgccgtcct accgcggtgg acgagcgcgg
     5281 aacgtggatg agatgatggc ggcgatccga tgatcggcac gatc
//
LOCUS       DATLBZ010000249        20804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1015502, whole genome shotgun sequence.
ACCESSION   DATLBZ010000249 DATLBZ010000000
VERSION     DATLBZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20804
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015502"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VMZ53_13145"
     CDS             <1..565
                     /locus_tag="VMZ53_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS29456.1"
                     /translation="ETIKVDVRVVAATNKDLHAEIAAGRFREDLYYRLAVVPIEMPPL
                     RARREDIHVLVEHFLEQVCADNERRPKKIASGAQTLLMQHDWPGNVRELKNVVERLAI
                     LTGDAEIITEADVGDALPRVKAVKAEFARGTPFKDLVAAAERDIIVAALDANDHHVSN
                     TARELQLERSHLYKKMRALGIDPREPE"
     gene            complement(573..863)
                     /locus_tag="VMZ53_13150"
     CDS             complement(573..863)
                     /locus_tag="VMZ53_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29457.1"
                     /translation="MERNGSELARYIQRYRSSLLGGIASAAVVALTLDQSSSAAHSSF
                     EFALWFAMIATLIAVVLTGMLSIAEYRAARPAMLPKATARPRRTISVEFIDN"
     gene            complement(943..2643)
                     /locus_tag="VMZ53_13155"
     CDS             complement(943..2643)
                     /locus_tag="VMZ53_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29458.1"
                     /translation="MRRKAWIAAVPLLAFAGCQDDTYLIVTVDKRPAVHQAAKLKITL
                     SNSGSMRTDDLDLAGHDFPVTFSLTAPGRGGELGISVDALDAGGALVGRGSGQATLSD
                     DTATVTLDSADFVVNSEYAGNQFLSNDFEAVGLQLAAITNGTWMPVFRDDCTGTPAAC
                     DIYGRRFDAAGLPVNSTLAAGTNQFKISTTLTSPGAIPAVASSGITTMVFWDYFDTVG
                     SGQGVACRAINEQGAGTPAQLSISTESADVVTTAPIGNGNFVVTWQTFMTQETIHALV
                     VKPDCTTLQATPINVSTSTISLGHRRSHVAGNGTGLLFVWVADDSVYARPAMNNGTLV
                     GVEVKPVAKTTSMVVDHVRVVPYGTGYALGVRWAAVDTSSPGKIDVYKLDAMGNVVGT
                     PTLITDKSGSDFASDKGFSMAARSDGALLVVWHTCSSGPGSCDVYGRVLRPTGVPVGE
                     EFMIPTANGSDQVNPSAVALADSFVAAWNDSSQLDPDKSMSAVRARVLYPPYDDARSV
                     LGATCGASAPGAPTCGPGLACANGSDQVQRCYQTCTPPSCPGGGTCSTVEGGMSACTF
                     "
     gene            complement(2643..3179)
                     /locus_tag="VMZ53_13160"
     CDS             complement(2643..3179)
                     /locus_tag="VMZ53_13160"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS29459.1"
                     /translation="MIPRLFLLMVVCLAVAAPTVARADDPATRAARRHYERGEKLFAL
                     GKFDESLEEYQEAFDAKPLPGFLYNIGQCYRNLGDYDQAIFSFKKYLKLQPDADNKDA
                     VEQLISDLEDKKARGDGAKFVRKKPKPVEDDKPITSKWWFWTGVGVVAVAGGAGIYSA
                     TRPSGPPTTDLGNIVFGK"
     gene            complement(3176..5278)
                     /locus_tag="VMZ53_13165"
     CDS             complement(3176..5278)
                     /locus_tag="VMZ53_13165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29460.1"
                     /translation="MTTRDPRSRVPLRPQTGPSESAREPTQSLSIEIDPADAESDSSL
                     HPSLAVGTSDAVREDRTRVESPNGRIKTPQPKPATKPPPTPRGGEPDYALGTVLGAYK
                     LVEMLGKGGMGFVYRAEHIKLGREVALKLLRGDYAKRRDAISRFFQEARTVNRVRHRN
                     IVDVTDLVELDDGTTYFVMELLRGQSLGKWARTGIDLPRALAVLVQICDGLGAAHAVG
                     VVHRDLKPDNVFVVPTSDGAELVKILDFGVAKLVNRDDEDVGYQTAAGSVIGTPAYMS
                     PEQAGGMLVDNRSDIYSLGAIMYEMFCGQPMFRGRSFGEYVRKHLTENPMPPRQTAGG
                     ASMDPNLEALIQKCLSKDPNERFGHILELRDGLLHLLGGIETHPPAYVAMSSIRTGPP
                     AAIATIPPLVPQPATQLALPSLPPHMLPLPPGMSTTVPTPPSITPSQSHFSQSHLTQS
                     SVQYSHYTMQPESAHTEPGTPWWVWFVGGAIAVGLGIAGALWYANSSSEPTPTAKEPI
                     TAPIENGSAVPPQAQLVELKFDSLPSAGVYADGRSAELCKTPCNFNVDLGDGGPTDKR
                     TFVVRTDGFKDRIVVVDFAAAQRAFSVTLDRVEATVPTVTDDTQTEEAVATTDDAQGG
                     DDKTPTSTKKKKKKKVSKTETPDPKAQGSAQIEEKQPPPPLPDDTSLEKVEKKPEEKK
                     PPGTIDPTDTIDPFSRKK"
     gene            complement(5390..6001)
                     /locus_tag="VMZ53_13170"
     CDS             complement(5390..6001)
                     /locus_tag="VMZ53_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29461.1"
                     /translation="MKAIAGAIVVAVLVALASGASPLPQQATNTLTAIDTVPTRQQID
                     QAFINSPQTPLQNLSFLATDTDAGTDIGVRLRAIHALAKYCTSTPCLPADEAHQTVRT
                     VVAGNAHELSGPRALILRAGIETLGAMRVGQDADFLVAQLSLLDHPSRDIRASTARAL
                     AELCSGPSAVNALRLRYSHELTEQVKLAISEALRILGQCTTNP"
     gene            complement(5998..7377)
                     /locus_tag="VMZ53_13175"
     CDS             complement(5998..7377)
                     /locus_tag="VMZ53_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29462.1"
                     /translation="MRAPECGDGFQCDAAGFCIAATGQEGDSCTSEVECAAGLACEIE
                     AAATMDTGKLVASCVPENAGRPTASVCSKDGDCRSGTCEIGRCIDLCNETRDCGAGTS
                     CTAMPRVAAQGHMFNGCLQSTGALSWTIPITGPASEVALPIPASARSLSVLFTVEDQN
                     QKVGATEIFAPGGLPLISPLQYSYFTNPFVRHRPELGQSVLAIPSTPLHDLQAGLYRL
                     TVRSLRPRVDPLDPNHLIDVTGTATPSMTAVIKLGYGRQLDLHFYFLNLDDHPCADSF
                     GGKLDATTAPDAPFFSNYLEELSRIFAPAATIEHVTYENLRDHPDLDGLDVANAPALL
                     SLGKHATGINVFFVRTLSPVGLQAIGPNPGPAGLAGTRQSGVIIALDSLCYRSWNELA
                     RITAHELSRYMGLYRNVEQDPVTPDVHIDPIDDSDRSSSNLMFPSELGGTELSFGQAD
                     ILSRSAVLR"
     gene            7608..8360
                     /locus_tag="VMZ53_13180"
     CDS             7608..8360
                     /locus_tag="VMZ53_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29463.1"
                     /translation="MTLIGLSGTAWGGAALDDAGSPQRTTDPNASPDTPATAEPKLEY
                     GVDIRLRHVMIPQGLVELFVERAAGGSGNNGIGLDLVRRRGNLELQLGFEFEHIEPGE
                     GVWINKGDNVAGGDEADYIISAEHAGKSLGWFTLEFTFLNHAPINKYLAVRYGGGAGI
                     GIITGELQHYDVICNGATNGNPEPGCVPPAFGGTPQSSTGPVAYDFPPIFPVVNAIIG
                     LQIRPAPKATINIEGGIRTVPFIGISAGYFFN"
     gene            complement(8476..10380)
                     /gene="selB"
                     /locus_tag="VMZ53_13185"
     CDS             complement(8476..10380)
                     /gene="selB"
                     /locus_tag="VMZ53_13185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HUS29464.1"
                     /translation="MLHPIVLGTAGHIDHGKTSLVRALTGIDTDRLPVEKQRGITTEL
                     GFARLDLDRGAGQQPQRVAVVDVPGHERFVKSMVAGATGLDLVMLVIAADEGVMPQTR
                     EHLDICDLLGVRRGLIVLSKKDLVDAEWLTMVTADVRTAVAGTFLAEATIVAVSTKDE
                     ATIEGLKRELSALIEKLPPRAQTGVFRLPIDRVFTVKGFGTIVTGTVLGGEVKLGDEL
                     AVIPSGLAPRVRGIEVHGAAVDKAIAGHRAALNLGGVAVEDLARGDLLAHPGKVAASH
                     ILDVELRYLRSAPGPLAARSKVLVHHGTAQVLAALHLVDKPTLAPGETGLAQLRLDQT
                     TPLGALPEDHFIVRGFVETSTGGSTIGGGRIIRVLAPKARKGEAHAQTVAALAAAKLD
                     GRIALDIKTSAFAGLSVADLVRRLGVPANELEPPLAKLTASRELLAIGDHYLHATAVA
                     ELEQKVSKLVGAAENGLPREDLRTQLPAALSARAYDAILAGLDKRGLVAAVGDRVTKS
                     AKGATAALSSVEQAVLSKLEQTGIEPPRPKELPASLGLAEPQVKTALDKLIAAKLAIK
                     VKPDLVMHAKTVADVRGKLVAHLERHGTIDAQQWKDLTGASRKFTIPLAEYFDAEKLT
                     LRIGDVRRKR"
     gene            complement(10385..12121)
                     /locus_tag="VMZ53_13190"
     CDS             complement(10385..12121)
                     /locus_tag="VMZ53_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUS29465.1"
                     /translation="MLDRLNKLRERAERVVTRAERAVFFASVLRASKVVSPMRPSAFA
                     QFAKSARQTKLGPHVAIMFHAAAHPEKEAIVEYGEHGVRRLTWGEFDATINRVAHALV
                     ARGVKGGSRVALMLPNGSEYLIAQQALARLGATAVQIGYRLKAGEIAFILENSGPTAT
                     IVYGEYVGTMLEARKQANKGGAMLVVGGEVDPARRSQSAGLHSGPGSDEIEDWDRALA
                     AASPEMPPRVRGGDGGGVIVYTSGTTGKPKGANRSWRKTGFESVADMILQVGMRADDR
                     HLVTCPLYHSAAPAFVAIMMSLGATCVLQNHFEPEAALDIIQKERITCSLMVPTMLIR
                     MSNLPPETRKKYDTSSLRWVMSGAAPLTTEAARRFMEQFGPVLWNFYGATETGLVTLA
                     GPHDHVSRPGTIGKKLRGNDIRLLDDSGSDVGPGQVGELYARNPTLISGYHGNDDATK
                     SSQRDGFFSVGDVGRIDDEGYYYLESRKHDMVISGGVNIYPREIEDHLSTHPAILEAA
                     VIGVPDPEWGETLRAFIVLRHGQSLTETEVVTYCREALADYKRPRKVTFMAELPRNPT
                     GKVLKRELRELA"
     gene            complement(12220..16272)
                     /locus_tag="VMZ53_13195"
     CDS             complement(12220..16272)
                     /locus_tag="VMZ53_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS29466.1"
                     /translation="MIEATCAACGTVARIAESDVPVGAKFVTCASCKARVVLPQQAAS
                     STPAKGIKSVSIPAIPKTPPPIPAKGRSDTIDLADLPAPKRSSPLAGAEPSKPAPRSA
                     LSGADLPAPKVAKPPAPAPGALDLDDLMPADLPAPKGKSDIGLADLPAPKPKASSLGD
                     LPAGKGKQASALTDLPAPKSKASALTDLPAPKAKSEALADLPTPKRSGLPPGKIPDVD
                     DGLDLPMPKPGANNDLPAPKGFFDDLPQPAKPSTGGAPTIDLPAPKGFFDDLPQPAKA
                     SAQTRGPGQEVAPKGFFDDLPQPAKGGGASTDVTPKGGGSQSVTPKGAGSQAVTPKGP
                     GQEVAPKGFFDDLPQPAKGGGKAGLFDDLPQPTKPAAGGLFDDLPQPSTSQDIPLELG
                     NGAGPDLDLGLPLGESGGFQDLDLGEPLKRGGAAPPDEPASPIKIKTPAKGAAPAKPI
                     PIVVPKDPKQGGELKLDLADDPHGGVGAPAATSAKKAAAGKKKGPTPEEAAEVRAAKR
                     RRSRIILGAVLGLAALGAGGFYVYQRQMKQKERREKIETGITGARAALKAEAPTHWAN
                     ALGQAQQVIDMDPNNTVALGLASEASFAGALDSGVNGPARIAQGRKFMTDGLGAGKVS
                     KELDRAQALSYVAANQFDKAIPRLQGLIQQDPTDGWLQLYLGWAELGAGDADAALKAF
                     DQAVAKTKQTKIPALYGHGRAKLLLADIAAARTDFETILQTSKDHVCAQVQLAATLEP
                     SKAAQRDADLKGILERKDVKAKQADPRCITQVYVNLGDVAASNGRLDIARDNYRKAIG
                     ATANDVDALVGLARVEIRDNKLPVAADHVQKALAANANAPHAQLANAELLVAQGKLPD
                     AQKQIDALAARKPPLVKLDQATLQVVKGKLLVALGKPEEAIEAYAEGAKLAGELDLTP
                     TMAAVTLLTDLAKKDETKAADYRKRADEMLSAFATRAQDDPQLSAQLGVAYLQAGDAT
                     KAETFLRRAVTMRDEDPEPKISLAIALSQLGRTDEALEQIQSAIKLDGKRVDFQLQLA
                     LTFQNAGRDAQAIEAYDKLLAMPDAPIIVRANAGKYFAKKGLLDRNLDLIKKGAVQSE
                     PILKAEPENAAGLYLKGEGLIQTGKFDEATPVLTKASDIDPDAQYLDALGRAYEGRIE
                     SDKKFIEAARNAYDRASKADPKLVHAMVGQGAMLVRQTHYDQALKPLLAAIALDRTNS
                     EIQFWIGEAYYGLRNVDKDYAKTGAQWLDTAVHKGVPELPIERRAEAAHDLGTLYDEK
                     LNNAAGCAAAWELAQSLGEQIIKDKGEAPALEKMRWFVEGYYYLGDTYAKMNNPVAQK
                     KAWLKYLERGKDTTHVKVVNQQLATNLQRY"
     gene            complement(16367..17017)
                     /locus_tag="VMZ53_13200"
     CDS             complement(16367..17017)
                     /locus_tag="VMZ53_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29467.1"
                     /translation="MIRWLVLALAFVLVTPAWAQPAPQQERREKIKKRIRALRAYTLT
                     EELSLDEKTAGKLFPMLAKYDDEFDKLLVARVDLEKQLEAAGTQTDTKAIDKLIDDAS
                     ANQKALWDTEVKRLAELRKILTPQQVARTLVVLPKMERQIQNQLRKAAAAKQKAQAAP
                     VDPYDDELRDPFAAHPKGKATKKPVDSGELSGNPYGPPKKQKKPCDPFGDARGCVE"
     gene            complement(17014..17508)
                     /locus_tag="VMZ53_13205"
     CDS             complement(17014..17508)
                     /locus_tag="VMZ53_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29468.1"
                     /translation="MRRPGEDDELDDRDHELIAKLRALPPEGTEPNWQKLEAAIRAEV
                     GDRAPRPWWRNWKWIIPVWALATTAAVALIVMRDHDAPAPQHALTSPSQHDTPSTQRE
                     VTNAPAMWLDGEALNLDDVDDASLDNLDQAAREVLDTDDVGLDELDDAALDEIEQWLE
                     KERS"
     gene            complement(17492..18049)
                     /locus_tag="VMZ53_13210"
     CDS             complement(17492..18049)
                     /locus_tag="VMZ53_13210"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS29469.1"
                     /translation="MTDAIDPDRELAEKFRQGDRSAFDVLVRRHQKGVWRIVRRYVKR
                     DADAADVTQLVFVRAFKALGGFRGAASVRSWLYRIAINCSLSWLRDHRREEPTEIAED
                     SLTELPIAPGRIASDQESAQLRKAIAQLPPKQRMVLELRVFDDLSFKEVAELADCSEN
                     TAKVSFHYAVKKLRDILGGDHASAR"
     gene            18179..19462
                     /gene="serS"
                     /locus_tag="VMZ53_13215"
     CDS             18179..19462
                     /gene="serS"
                     /locus_tag="VMZ53_13215"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HUS29470.1"
                     /translation="MLDPARMDDVLRRGGDVATVVGSWKSLDERRRKLQGDLDTLRQQ
                     RNAANDKMSKLDKKSDEFKAAVAEMKGVSTKIKEGEAELGTVEQEASALLLTIPNAPH
                     ESVPNGTSEHDNVELHVWGTKPTFSFAPKTHADLGPSLGLDFEAGAKITGSRFTVLRG
                     AASRLTRGLINYMLDLHTQKHGYGEIWPPAIVKRASLRGTGQLPKFEADLFRLDIPVG
                     PDHVPDNDLFLSPTAEVQVTNLYNDHIFEAGELPVRLTAYSPCFRAEAGAGGKDTRGL
                     IRQHQFDKVELVKFVEPEHSYGELEALRQDAEAVLQGLGLHYRVMTLCSGDMGFASAK
                     TYDLEVWLPGQDAFREISSCSNFESFQARRAGIRYRTEPKAKPRAVHTLNGSGIAIGR
                     TIVAIIEQYQQADGTVVIPAALRPYVGGMERLTPV"
     gene            19548..19630
                     /locus_tag="VMZ53_13220"
     tRNA            19548..19630
                     /locus_tag="VMZ53_13220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19582..19584,aa:Ser,seq:tga)
     gene            19752..19842
                     /locus_tag="VMZ53_13225"
     tRNA            19752..19842
                     /locus_tag="VMZ53_13225"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19786..19788,aa:Ser,seq:gct)
     gene            19861..19933
                     /locus_tag="VMZ53_13230"
     tRNA            19861..19933
                     /locus_tag="VMZ53_13230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19894..19896,aa:Arg,seq:acg)
     gene            19955..20431
                     /gene="tadA"
                     /locus_tag="VMZ53_13235"
     CDS             19955..20431
                     /gene="tadA"
                     /locus_tag="VMZ53_13235"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HUS29471.1"
                     /translation="MTTTDDQWMQVALEEARAAAAAGDVPVGAVIVGSDGALLGRGRN
                     RRELDKDPTAHAEIVALRAAAATLGMWRVEATLFVTQEPCPMCAGALVNARVTRLVYG
                     CANPKAGAVASLFSLVTDSRLNHRMAVTSGVLATECAAVLSQFFADLRRGPTYGGS"
     gene            20439..20521
                     /locus_tag="VMZ53_13240"
     tRNA            20439..20521
                     /locus_tag="VMZ53_13240"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20473..20475,aa:Ser,seq:cga)
     gene            20549..20633
                     /locus_tag="VMZ53_13245"
     tRNA            20549..20633
                     /locus_tag="VMZ53_13245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20583..20585,aa:Ser,seq:gga)
     gene            20689..20786
                     /gene="ffs"
                     /locus_tag="VMZ53_13250"
     ncRNA           20689..20786
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VMZ53_13250"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT         3365 a         6738 c         6919 g         3782 t
ORIGIN      
        1 cgagacgatc aaggtcgacg tgcgcgtcgt cgcggcgaca aacaaggatc tgcacgccga
       61 gatcgccgcg ggccgctttc gcgaggatct ctactaccgc ctcgccgtcg tgccgatcga
      121 gatgccgcct ctgcgcgcgc ggcgcgaaga tatccacgtg ctcgtcgagc attttctcga
      181 gcaggtctgc gccgacaacg agcgccggcc caagaagatc gcgtcgggcg cgcagacgct
      241 gctcatgcag cacgactggc cgggcaacgt gcgcgagctg aagaacgtcg tcgagcggct
      301 cgccattctc acgggtgacg cggagatcat caccgaggcc gacgtcggcg atgcgctacc
      361 gcgcgtcaaa gcggtcaagg ccgagttcgc acgcggcacg ccattcaagg atctcgtcgc
      421 tgcagcggag cgcgacatca tcgtcgctgc gctcgacgca aacgatcatc acgtgtcgaa
      481 caccgcccgc gagctgcagc tcgagcgcag ccatctctac aagaagatgc gcgcgctcgg
      541 gatagatccg cgcgagcctg agtgaagcgg cgctagttgt cgatgaattc gacgctgatc
      601 gtgcggcgcg gccgcgcggt cgctttcggc agcatggcgg gacgcgctgc gcggtactcc
      661 gcgatcgaga gcatgccggt cagcacgaca gcgatcagcg tcgcgatcat cgcgaaccac
      721 agggcgaact cgaagctcga gtgcgcggcg ctggagctct gatcgagcgt gagcgcgacg
      781 acggccgctg aggcgatgcc accgagcaag gagctgcggt agcgctggat gtagcgagca
      841 agctcagacc cgttgcgttc caccgtccat catcgtcgat gatctggccg ggcgagacaa
      901 gcagattgtc gtcggagaac ttccgagcag cgacgccggc gactagaacg tgcaggcgga
      961 catgccgccc tcgaccgtcg agcaggtgcc gccaccgggg cacgacggcg gcgtgcacgt
     1021 ctgatagcag cgctggacct ggtcggagcc gtttgcgcag gccaggccgg ggccgcaggt
     1081 cggggcgccg ggggcgctcg caccgcacgt cgctccgagg acgctgcggg cgtcgtcgta
     1141 cggcgggtag agcacgcggg cacggacggc cgacatcgac ttgtcggggt cgagctggct
     1201 cgagtcgttc cacgcggcga cgaaggaatc cgcgagcgcg acggccgacg ggttgacctg
     1261 gtcgctgcca ttcgcggtcg ggatcatgaa ctcctcgccg acggggacgc cggtcggccg
     1321 aagcacgcgg ccgtacacgt cgcacgagcc aggcccgctc gagcaggtgt gccagacgac
     1381 gagcagtgcg ccgtccgagc gagccgccat cgagaagccc ttgtcggacg caaagtccga
     1441 gccgctcttg tcggtgatga gcgtcggcgt accgacgacg ttgcccatgg cgtcgagctt
     1501 gtagacgtcg atcttgccgg ggctcgacgt atcgaccgcc gcccaccgaa caccgagcgc
     1561 gtagccggtg ccgtacggaa cgacgcgcac gtgatcgacg accatcgacg tggtcttcgc
     1621 gaccggcttc acttccacgc cgacgagcgt gccgttgttc atcgccgggc gcgcgtacac
     1681 ggaatcgtcc gccacccaca cgaacagcag gcccgtaccg ttgccggcga catgcgagcg
     1741 gcggtgaccg agcgagatcg tgctcgtcga cacgttgatc ggcgtcgctt gcagtgtcgt
     1801 gcagtccggc ttcacgacga gcgcgtgaat cgtctcctgc gtcatgaacg tctgccacgt
     1861 gacgacgaag ttgccgttgc cgatcggcgc ggtggtcacg acgtcggccg attccgtcga
     1921 gatcgacagc tgcgccggcg tcccagcgcc ctgctcgttg atcgcgcgac acgcgacgcc
     1981 ctgcccactt ccgacggtgt cgaagtaatc ccagaacacc atcgtcgtga tgcccgacga
     2041 cgcgaccgcg ggaatcgcgc cgggcgacgt cagcgtcgtc gagatcttga actgattcgt
     2101 gccggcggcg agcgtcgaat tcacgggcag ccctgcggcg tcgaaccggc ggccgtagat
     2161 gtcacatgcc gcaggcgtgc ccgtgcagtc gtcgcgaaag accggcatcc acgtgccgtt
     2221 cgtgatcgct gcgagctgca ggccgacggc ctcgaagtcg ttcgacagga actgattgcc
     2281 ggcgtactcg ctgttgacga cgaagtccgc gctgtcgagc gtgaccgttg cagtgtcgtc
     2341 gctcagcgtc gcttgcccgc tgccgcgacc gacgagtgcg ccgcccgcat cgagtgcgtc
     2401 gacggagatg ccgagctcgc caccgcggcc gggagccgtg aggctgaatg tcacggggaa
     2461 gtcgtgaccc gcgagatcga gatcgtcggt gcgcatcgat cccgagttcg acagcgtgat
     2521 cttgagcttc gccgcttggt gcacggccgg ccgtttatcg acggtgacga tcaagtacgt
     2581 gtcgtcctgg cagcctgcaa aggccaacag cgggacggcg gcgatccaag ccttgcggcg
     2641 catcactttc cgaagacgat gttaccgagg tccgtcgtcg gtgggccact cggacgcgtt
     2701 gctgagtaga tgccagcacc gccggcgacc gcaacgactc cgacaccggt ccaaaaccac
     2761 catttcgagg tgatcggctt gtcatcctcg acgggctttg gcttcttgcg aacgaacttg
     2821 gcgccatcgc cgcgcgcctt cttgtcctcg agatccgaga tcagctgctc gaccgcatcc
     2881 ttgttatccg cgtcgggctg gagcttcagg tacttcttga agctgaagat cgcttggtcg
     2941 tagtcgccga ggtttcggta gcactggccg atgttgtaga ggaagccagg cagcggcttg
     3001 gcgtcgaacg cttcctgata ctcctcgagg gactcgtcga acttgccgag tgcgaacagc
     3061 ttctcgccac gctcgtagtg gcggcgggcc gcgcgggttg caggatcatc tgcgcgcgcc
     3121 accgtcggag cggcgacggc gagacacacc accatcaaga gaaataggcg aggaatcatt
     3181 ttttgcgaga gaacggatcg atcgtatccg tggggtcgat cgtacccgga ggcttcttct
     3241 cttcgggctt cttctccact ttttcgaggc tcgtatcgtc tggcagcggc gggggcggct
     3301 gtttctcttc gatttgcgcg ctgccctggg ccttcggatc cggggtttcc gtcttcgaga
     3361 ccttcttttt cttcttcttc ttggtcgacg tcggcgtctt gtcgtctccg ccctgcgcgt
     3421 cgtcggtcgt cgcgacggct tcttccgtct gcgtgtcgtc ggtcacggtc ggcacggttg
     3481 cctcgacgcg atcgagcgtg accgagaatg cgcgctgagc cgccgcgaaa tcgacgacga
     3541 cgatgcgatc cttgaacccg tccgtgcgga cgacgaacgt gcgcttgtcc gtcgggccgc
     3601 cgtcgccgag atcgacgttg aagttgcacg gcgtcttgca gagctccgcc gagcggccgt
     3661 ctgcatagac gcctgcgctc ggcagcgaat cgaacttgag ctcgacgagc tgcgcttgcg
     3721 gcggcaccgc agagccgttc tcgatcggcg ctgtgatcgg ttccttcgcc gtcggtgtcg
     3781 gctcgctcga gctgttcgcg taccagagtg cgccggcgat cccgaggcca acggcgatcg
     3841 caccgccgac gaaccacacc caccacggcg tccccggctc ggtgtgcgcc gactcgggct
     3901 gcatcgtgta gtggctgtat tgcaccgacg actgcgtgag gtgcgattgc gagaagtgcg
     3961 attgcgatgg cgtgatcgac ggaggcgtcg gcacggtcgt cgacatgccc ggcggcagcg
     4021 gcagcatgtg cggcggcagg ctcggcagcg cgagctgggt cgccggttgc ggcacgagcg
     4081 gcgggatcgt cgcgatcgca gcgggcggtc cggtgcggat gctcgacatc gcgacgtagg
     4141 caggaggatg cgtctcgatg ccgccgagca ggtgcagcag gccgtcacgc agctcgagga
     4201 tgtggccgaa ccgttcgttc ggatctttcg agagacactt ctggatcagc gcctcgagat
     4261 tgggatccat gctcgcgccg cccgccgttt gccgcggtgg catcggattc tcggtgaggt
     4321 gcttgcgcac gtactcgccg aacgagcggc cgcggaacat cggctggccg caaaacatct
     4381 cgtacatgat cgcgccgagc gagtagatat cgctgcggtt gtcgacgagc atgccgcccg
     4441 cttgctccgg gctcatgtat gcgggcgtgc cgatcaccga gcctgcagcg gtctggtagc
     4501 cgacgtcctc gtcgtcgcgg ttgacgagct tggccacgcc gaaatcgagg atcttcacga
     4561 gctcggcacc gtcggaggtc ggcacgacga acacgttgtc gggcttgaga tcgcgatgca
     4621 cgacgccgac cgcgtgtgcg gcaccaaggc catcgcagat ctgcacgagc acggcgagcg
     4681 cgcgcggtag atcgatgccg gtgcgcgccc acttgccgag gctctgcccg cgcagcagct
     4741 ccatcacgaa gtacgtcgtg ccgtcgtcga gctcgacgag atcggtgacg tcgacgatgt
     4801 tgcgatggcg aacgcgattg acggtgcgcg cctcttggaa gaagcggctg atcgcatcgc
     4861 gacgtttcgc gtagtcgccc cgcaggagct tcagcgcgac ctcgcggccg agcttgatgt
     4921 gctccgcgcg atagacaaag cccatcccgc ctttgccgag catctcgacg agcttgtagg
     4981 cgccgagcac ggtgccgagc gcgtagtcgg gctcgccacc gcgcggagtc ggcggcggct
     5041 tggtagcggg cttgggttgc ggagtcttta tgcggccgtt cggggactcg accctcgtgc
     5101 gatcctcgcg cactgcgtcg ctcgtcccga ccgcgagtga gggatgcagc gagctgtcgg
     5161 actcggcatc ggcgggatcg atctcgatcg acagcgactg tgtcggttcg cgtgcgctct
     5221 cgctaggccc ggtttgcggg cgcaacggta cgcgtgaacg cgggtcgcgg gtcgtcatgg
     5281 cacctcacag cccgcgtgat cagcgcagac gtgtagcgag gtaacacgaa ttgtacgatc
     5341 agcacccctg ctgccgcaaa ccaaacattt cgagtgtttg tgcgctactt cacgggtttg
     5401 tcgtgcactg acccaaaatc cgaagagcct ctgaaatcgc tagcttcacc tgctccgtca
     5461 gctcgtggga gtagcgaagg cgcaaggcat tgactgcact gggcccgctg cacagctcgg
     5521 cgagggccct cgcggtcgac gcccggatgt cccgcgatgg gtggtcgagc agcgagagtt
     5581 gggcgaccag gaagtccgcg tcctgaccga ctcgcatcgc gccgagcgtc tcgatacccg
     5641 cgcgaaggat cagggcgcgc ggtccactga gctcgtgcgc gttgccggcg accacggtgc
     5701 gcacggtttg atgagcttca tccgcgggca agcatggtgt cgacgtgcag tacttcgcga
     5761 gcgcgtgaat ggcgcgaagg cgaacgccga tatcggtccc ggcatcggtg tcggtcgcca
     5821 ggaacgacag gttctgcagc ggtgtctgcg gcgagttgat gaatgcctga tcgatctgct
     5881 gccgcgtcgg cacggtgtcg atcgcggtga gcgtgttcgt cgcttgttgc ggcagtgggc
     5941 tcgcgccgct ggcgagcgca acgagcacgg ccacgacgat ggcgcctgcg atcgccttca
     6001 tcgcagcacc gcgctgcggc tgaggatatc cgcttggcca aacgagagct ccgtgccgcc
     6061 gagctcgctc gggaacatca ggttcgacga cgaccggtcg ctatcgtcga tcggatcgat
     6121 gtgaacatcg ggcgtgaccg ggtcttgctc gacgttgcga tagagaccca tgtagcgcga
     6181 cagctcgtgc gcggtgatcc gtgcgagctc gttccacgag cgataacaga gcgagtcgag
     6241 cgcgatgatc acgccggatt gacgcgtccc tgcgagaccg gcgggaccgg gattcggccc
     6301 gatcgcctgc aaaccgacgg gcgagagcgt gcgaacgaag aagacgttga tgccggtcgc
     6361 gtgcttgccg agcgagagga gcgcgggcgc gttcgcgacg tcgagaccgt cgaggtcggg
     6421 atggtcgcgc aggttctcat acgtgacgtg ctcgatcgtt gccgccggcg cgaagatgcg
     6481 agacagctcc tcgaggtagt tgctgaagaa cggcgcatcg ggggcagtcg tcgcgtcgag
     6541 cttgccgccg aacgaatccg cgcacggatg gtcgtcgagg ttcaagaagt agaagtggag
     6601 gtcgagctgg cgcccatagc cgagcttgat caccgccgtc atcgacggtg tcgccgtgcc
     6661 ggtgacgtcg atgaggtgat tgggatcgag tggatcgacg cgcggccgca gcgagcgcac
     6721 cgtcagccgg tagaggccgg cctgcagatc gtggagcggc gtcgacggaa tcgcgagcac
     6781 ggactggccg agctccgggc gatgccggac gaatggattc gtgaagtagc tgtactgcag
     6841 cggcgagatc agcggcagcc caccgggggc gaagatctcc gtcgcgccta ccttctgatt
     6901 ctgatcctcg acggtaaaca gcaccgacag cgatcgcgcc gacgcgggaa tcggcagtgc
     6961 gacctcggac gcgggccccg tgatcggaat cgtccagctc agcgcgccgg tcgactgcaa
     7021 gcagccgttg aacatgtggc cttgcgccgc aacgcgaggc atcgcggtgc acgacgtgcc
     7081 cgcgccgcaa tcgcgcgtct cgttgcacag gtcgatgcag cgcccgatct cgcacgttcc
     7141 gcttcgacag tcgccgtctt tgctgcagac cgacgccgtg ggccgtccgg cattctccgg
     7201 cacacagctc gcgaccagct tgccggtatc catcgtcgcc gccgcttcga tctcgcacgc
     7261 cagcccggcc gcgcactcga cctcgctcgt gcacgaatcg ccctcttgcc ccgtcgctgc
     7321 gatgcaaaac cccgctgcat cgcactgaaa tccatcgccg cattcgggtg cgcgcacgca
     7381 ccggggaacg cacgtgcctg cgaagcactg cagctcgctt ttgcattcgt tctggctcgc
     7441 acaccgatcg ccgatcaacc cgtcgccgcc tgggcaccct gcgagggtga ggacggagaa
     7501 gatcagtgtg cgaagtcgca tagcgtttct cgccggaatc gcgggactct gacgcccgga
     7561 gtatatattg tcgcctcgcc gggggaatca tgcgtttagc accgctcgtg actttgatcg
     7621 gcctttcggg aactgcatgg ggtggagccg cgctcgacga tgcgggatct ccgcagcgga
     7681 caacggatcc caacgcatct ccggatactc cggcgaccgc cgagcccaag ctcgagtacg
     7741 gcgtcgatat ccgactgcga catgtgatga tcccgcaagg cctcgtcgag ctgtttgtcg
     7801 agcgagccgc aggtggctcg ggtaacaacg gcatcggcct cgacctcgtt cgccgccgcg
     7861 gcaatctcga gctccagctc ggcttcgagt tcgagcacat cgagcccggc gaaggcgtct
     7921 ggatcaacaa gggcgacaac gtggcgggcg gcgacgaagc cgactacatc atcagcgccg
     7981 agcacgccgg caagagcctc ggctggttca cgctcgagtt cacgttcctg aaccacgcgc
     8041 cgatcaacaa gtacctcgcg gttcgctacg gcggtggcgc aggtatcggc atcatcacgg
     8101 gcgagctgca gcactacgac gtgatctgca acggcgcgac gaacggcaat cccgagccag
     8161 gctgcgttcc gcccgcattc ggtggcacgc cgcagagctc gacgggccca gtcgcgtacg
     8221 acttcccgcc gatcttcccg gtcgtcaacg cgatcatcgg tctgcagatc cggcccgcgc
     8281 cgaaggcgac gatcaatatc gagggtggca tccgcacggt gccgttcatc ggtatctccg
     8341 ccggctactt cttcaactga gcgtcgtcgt cgcccgatca accgatcggt ctgaccacta
     8401 agggacggac gcaacttccg caacctgcgc aaccacgagg gacggaccgc gtccggtcgg
     8461 cggccacgtt cggagttagc gtttgcggcg aacgtcgccg atgcggagcg tgagcttctc
     8521 ggcgtcgaag tattcggcga gcgggatcgt gaatttgcgc gacgcgccgg tcagatcttt
     8581 ccactgctgc gcgtcgatcg tgccgtgacg ctcgagatgc gcgacgagct tgccgcggac
     8641 gtccgcgacc gtctttgcgt gcatcacgag gtcgggcttc accttgatcg cgagcttcgc
     8701 ggcgatcagc ttgtcgagcg cggtcttcac ttgcggctcg gcgaggccga gtgaggccgg
     8761 cagctctttc gggcgcggcg gctcgatgcc ggtctgctcg agcttcgaga gcacggcttg
     8821 ctcgaccgat gagagggccg cggttgcgcc tttcgccgat ttggtcacgc gatcgccgac
     8881 cgcagcgacg aggccgcgtt tgtcgagacc ggcgaggatc gcgtcgtatg cgcgagcgga
     8941 cagtgcggct ggcagctgcg tgcggaggtc ttcgcgaggc aggccgtttt cagccgcacc
     9001 gacgagcttg ctcacctttt gctcgagctc ggcaaccgcg gtcgcgtgca ggtagtgatc
     9061 gccgatcgcg aggagctcgc gcgatgcagt gagcttggcg agcggcggct cgagctcgtt
     9121 ggccggcacg ccgaggcgac ggacgaggtc ggcgacagag aggcctgcga acgcagacgt
     9181 cttgatgtcg agcgcgatgc gaccgtcgag cttcgcggcg gcgagggcgg cgacggtctg
     9241 cgcgtgtgct tcacctttgc gggctttcgg ggcgagcacg cggatgatgc gaccgccgcc
     9301 gatcgtcgag ccacccgtcg acgtctcgac gaaaccgcgc acgatgaagt gatcctcggg
     9361 cagcgcgccg agcggcgttg tctggtcgag gcgtagctgc gcgaggcccg tttcgccggg
     9421 cgcgagcgtc ggtttgtcga cgagatgcag cgcggccagc acctgcgcag ttccgtgatg
     9481 gacgagcacc ttgctgcgcg cggcgagcgg gcccggcgcg gagcgcaggt agcgcagctc
     9541 gacgtcgaga atgtgcgacg ccgcgacttt gccgggatgc gcgagcaagt ctccgcgcgc
     9601 cagatcttcc acggcaacgc cgccgaggtt cagcgccgcg cgatgtcctg cgatcgcttt
     9661 gtcgaccgcg gcgccatgga cttcgatgcc tcgcacgcga ggtgccagcc ccgacgggat
     9721 cactgcgagc tcgtcgccga gcttgacctc gccgccgagg acggtgccgg tgacgatcgt
     9781 gccgaagcct ttcaccgtga atacgcgatc gatcgggagg cggaacacgc cggtctgcgc
     9841 gcgcggaggc agcttctcga tcagcgcgga cagctcgcgc ttcagccctt cgatcgtcgc
     9901 ctcgtccttc gtcgacaccg cgacgatcgt cgcctcggcg agaaacgttc ccgcgacggc
     9961 agtgcggaca tcggccgtaa ccatcgtcag ccactcggcg tcgacgaggt ctttcttcga
    10021 aagcacgatg aggccgcgcc gcacgcctag cagatcgcaa atatcgaggt gctctcgcgt
    10081 ctgcggcatg acgccttcgt cggccgcgat cacgagcatg acgagatcga ggccggtcgc
    10141 gccggcgacc atcgacttca cgaatcgctc gtggcctggc acgtcgacga ccgccacgcg
    10201 ctgcggctgt tggcctgctc cgcgatcgag atcgagccgc gcgaacccga gctcggtcgt
    10261 gatgccgcgc tgcttctcga ccggcaagcg atcggtatcg atgcccgtca aggcgcggac
    10321 gagcgacgtc ttgccgtgat cgatgtgccc ggcagtgccg aggacgatcg ggtggagcac
    10381 ggctctacgc tagctcgcgc agctcgcgct tcaagacctt gcccgtcgga ttccgcggca
    10441 gctctgccat gaacgtgact ttgcgagggc gcttgtagtc agcgagagcc tcgcggcagt
    10501 acgtcacgac ctcggtctcc gtcagcgatt ggccgtggcg caagacgatg aacgcgcgga
    10561 gcgtctcgcc ccactcggga tcgggcacgc cgatcaccgc ggcctcgagg atcgcgggat
    10621 gcgtgctcag gtgatcttcg atctcgcgtg gatagatgtt cacgccgccg gagatcacca
    10681 tgtcgtgctt gcgcgactcg agatagtagt aaccctcgtc gtcgatccgg ccgacatctc
    10741 cgaccgagaa gaagccgtcg cgctgcgagg acttcgttgc gtcatcgttg ccgtgatagc
    10801 cgctgatgag cgtcgggtta cgcgcgtaga gctcgccgac ctggccgggg cctacgtcac
    10861 tgccgctgtc gtcgagcagc ctgatgtcgt tgccgcgcag tttcttgccg atcgtgcccg
    10921 gccgcgacac gtgatcgtgc gggcccgcga gcgtgacgag acccgtctcc gtcgcgccgt
    10981 agaagttcca cagcaccgga ccgaactgct ccatgaagcg gcgcgcagcc tccgtcgtga
    11041 gcggcgctgc gcccgacatc acccagcgca ggctcgacgt gtcgtacttc ttgcgcgtct
    11101 cgggcggcag gttcgacatg cggatcagca tcgtcggcac catcagcgag cacgtgatgc
    11161 gctccttctg aatgatgtcg agcgcggcct ccggctcgaa gtggttctgc agcacgcacg
    11221 tcgcgccgag cgacatcatg atcgcgacga acgccggcgc cgccgagtga tagagcgggc
    11281 acgtgacgag gtgacgatcg tcggcgcgca ttccgacctg caggatcatg tcggcgaccg
    11341 actcgaagcc ggtcttgcgc cacgagcgat tcgcgccctt cggtttgccg gtcgtgcccg
    11401 acgtatagac gatgacaccg ccgccatcgc cgcctctcac gcgcggaggc atctccggcg
    11461 acgcggcagc gagcgcgcgg tcccagtctt cgatctcgtc ggagcctggc ccggaatgga
    11521 gcccggcgga ttgactccga cgggcgggat cgacctcgcc gccgactacg agcatcgcgc
    11581 cgcccttgtt cgcctgcttg cgcgcttcga gcatcgtgcc gacgtactcg ccgtagacga
    11641 tcgtcgcggt cgggcccgag ttctcgagga tgaatgcgat ctcgccggcc ttgagccggt
    11701 agccgatctg cacggcagtt gcgccgagcc gcgcgagcgc ttgctgcgcg atgaggtact
    11761 cgctgccatt cggcaacatc agcgcaacgc gcgacccgcc cttcacaccg cgcgcgacga
    11821 gtgcatgcgc gacccgattg atcgtcgcgt cgaactcgcc ccacgtcagg cgccgaacgc
    11881 cgtgctcacc gtactcgacg atcgcttctt tttcggggtg tgccgccgcg tgaaacatga
    11941 tcgcgacgtg cgggccgagc ttcgtctggc gcgcgctctt ggcgaactgc gcgaacgccg
    12001 acggccgcat cggtgacacg accttgctcg cacgcagcac gctcgcgaag aacaccgcgc
    12061 gttcggcgcg cgtaacgacg cgctctgcgc gctcacggag cttgttcaat cggtccagca
    12121 cggacgcagc atatcccggc ctgcccctcg aagatcgcaa cagtcacgtt gcaacggctg
    12181 gccagtcgag tagccacaac ggcgctaact gcgcgtcgtc taatagcgct gcaggttcgt
    12241 cgcgagctgc tggttcacga ccttcacgtg ggtcgtgtct ttgccgcgct cgaggtactt
    12301 cagccacgcc ttcttctgcg cgacagggtt gttcatcttc gcgtacgtat cgccgaggta
    12361 gtagtacccc tcgacgaacc agcgcatctt ctcgagtgca ggcgcctcgc ccttgtcctt
    12421 gatgatctgc tcgccgagcg actgtgcaag ctcccacgcc gccgcgcacc ctgcagcgtt
    12481 gttgagcttc tcgtcgtaga gcgtgccgag atcgtgtgct gcttcggcgc gacgctcgat
    12541 ggggagctcg gggacgccct tgtgcacggc ggtatcgagc cactgcgcac cggtctttgc
    12601 gtagtctttg tcgacgttcc gcagcccgta gtaggcctcg ccgatccaga actggatctc
    12661 gctgttggtt cgatcgagcg caatggccgc gagcaacggc ttgagcgcct gatcgtagtg
    12721 cgtctgccgc acgagcatcg cgccctggcc gaccatcgcg tgcacgagct tggggtctgc
    12781 tttcgaagcg cggtcgtacg cgttgcgcgc cgcctcgatg aacttcttgt cgctctcgat
    12841 gcggccttcg tacgcacggc cgagcgcgtc tagatactgc gcgtcgggat cgatgtcgct
    12901 cgccttggtg agcaccggcg tcgcttcgtc gaacttgccg gtttgaatga gcccttcgcc
    12961 cttgaggtag agacccgcgg cgttctccgg ctccgccttc aagatcggct cggactgcac
    13021 agcgcccttc ttgatgaggt cgaggttgcg atcgagcagg cccttcttgg cgaagtactt
    13081 gccggcgttc gcgcgcacga tgatcggtgc gtccggcatc gcgagcagct tgtcgtacgc
    13141 ctcgatcgcc tgcgcatcgc gccccgcgtt ctggaacgtc agtgcgagct ggagctggaa
    13201 gtcgacgcgc ttgccgtcga gcttgatcgc gctctgaatc tgctcgagcg cttcgtcggt
    13261 gcggccgagc tgcgacagcg cgatcgcgag cgagatcttc ggttccggat cctcgtcgcg
    13321 catcgtcacc gcgcggcgca agaacgtctc tgccttcgtc gcatcgcctg cttgcagata
    13381 cgcgacgccg agctgcgccg agagctgcgg atcatcctgc gcgcgcgtcg caaacgcgga
    13441 gagcatctcg tctgcgcgct tgcggtagtc ggctgctttc gtctcgtcct tcttcgcgag
    13501 gtcggtcagc aatgtgaccg cggccatcgt cggcgtcaga tccagctcgc cggcgagctt
    13561 cgcgccctcg gcgtaagctt cgatcgcctc ctccggcttg ccgagcgcga cgagcagctt
    13621 gcccttcacg acctgcagcg tcgcctggtc gagcttcacg agcggaggct tgcgtgcggc
    13681 gagcgcatcg atctgcttct gcgcatcggg tagcttgcct tgcgcgacga gcagctcggc
    13741 gtttgcgagc tgggcatgcg gtgcattcgc gttggcggcg agcgctttct gcacgtggtc
    13801 cgcagcgact ggcagcttgt tgtcgcgaat ctcgacgcgg gcgagaccga cgagcgcatc
    13861 cacgtcgttc gcggtcgccc cgatcgcctt gcgatagttg tcgcgcgcga tatcgagacg
    13921 gccattcgat gcggccacat cgccgaggtt cacgtagacc tgcgtgatgc agcgagggtc
    13981 ggcctgcttc gccttgacgt ccttgcgctc gaggatgccc ttgagatcgg cgtcccgctg
    14041 cgcggccttc gatggttcga gcgtcgccgc gagctgcacc tgcgcgcaga catgatcctt
    14101 cgaggtctgc aggatcgtct cgaagtcggt gcgcgcagcc gcgatgtcgg cgagcaagag
    14161 cttcgcgcgg ccgtgaccgt agagcgccgg aatcttcgtc tgcttcgtct ttgcgacggc
    14221 ttgatcgaat gccttcagcg ccgcatcggc gtcgcccgca ccgagctctg cccagcctag
    14281 atacagctgc agccagccat cggtcgggtc ctgctggatg aggccctgca accgaggaat
    14341 cgccttgtcg aactgatttg cggcgacgta cgacaacgct tgcgcgcgat cgagctcctt
    14401 cgagactttg ccggcaccga ggccgtccgt catgaacttc cgcccctgcg cgatgcgagc
    14461 gggaccattc acgccgctat cgagcgcgcc cgcgaacgac gcctcggatg cgaggccgag
    14521 cgcgaccgtg ttgttcggat ccatgtcgat gacttgctga gcttggccga gtgcgttcgc
    14581 ccagtgcgtc ggagcttctg ccttgagagc cgcgcgcgcg ccggtgatgc cggtctcgat
    14641 cttctcgcgg cgctccttct gtttcatctg ccgctggtag acgtagaagc cgcctgcgcc
    14701 gagcgcggca aggccaagca ctgcaccgag gatgatgcga gaacgacggc gcttcgccgc
    14761 gcggacttcc gccgcctcct cgggggtcgg gcccttcttc ttgccggcag ccgccttctt
    14821 cgcagacgta gcggcaggtg cgccgacgcc gccgtgagga tcgtcggcga ggtcgagctt
    14881 gagctcccct ccttgcttcg gatccttcgg cacgacgatc gggatcggct ttgccggtgc
    14941 ggcgcccttc gcaggcgtct tgatcttgat cggggatgcg ggctcgtcgg gcggagctgc
    15001 gccgcctctc ttcagcggct cgccgagatc gagatcctga aagccgcctg attcgccgag
    15061 cgggagaccg agatcgagat cgggccccgc gccgttgccg agctcgagag gaatgtcctg
    15121 ggaggtcgat ggctgcggca gatcatcgaa caggccacct gcggccggct tcgtcggttg
    15181 cggcaggtca tcgaagaggc cggccttgcc gccgcccttt gcgggttgcg gaagatcgtc
    15241 gaagaatccc tttggcgcga cctcttgccc agggcccttc ggcgtgacgg cctgcgagcc
    15301 cgcacccttc ggcgtgacgg attgcgaccc accgcccttc ggcgtgacgt cggtgctagc
    15361 tccgccaccc ttcgcaggct gcggcagatc gtcgaagaat cccttcggcg cgacctcctg
    15421 accgggcccg cgtgtttgcg cactggcctt cgcaggctgc ggcagatcgt cgaagaaacc
    15481 tttcggcgcg ggcagatcga tcgtcggcgc gccacccgtc gatggcttcg cgggctgcgg
    15541 aagatcgtcg aagaatccct tcggcgccgg cagatcattg ttggcgccgg gcttgggcat
    15601 cggcagatcg aggccgtcgt cgacgtcggg gatcttgccg ggcggcaggc cgctgcgttt
    15661 cggcgttggg agatcggcga gcgcttcgga cttggccttc ggcgcgggca gatccgtgag
    15721 agccgacgcc ttcgatttgg gcgcggggag atcggtgagc gcggatgctt gcttgccctt
    15781 gcccgccggg agatcgccga gcgacgacgc cttcggcttg ggcgccggca gatcggcgag
    15841 gccgatatcg ctcttgccct tcggcgcggg caaatccgcc ggcatcagat cgtcgaggtc
    15901 gagcgcacca ggcgcaggcg cgggaggctt cgcgactttt ggagctggca ggtcggcccc
    15961 cgagagcgcg gaacgcggcg caggcttgct gggttcggca cccgcgagcg ggctactgcg
    16021 cttcggcgcc gggagatccg cgagatcgat cgtgtcgctc ctgcccttcg caggaatcgg
    16081 cggcggcgtc ttcggaatcg ccggaatcga gacgctcttg ataccctttg caggcgtcga
    16141 gctcgccgct tgctgcggca gtaccacgcg cgctttgcag ctcgcgcagg tcacaaactt
    16201 ggcgcccaca gggacgtccg attcggcgat gcgggccacc gtcccgcagg ccgcacaagt
    16261 ggcctcgatc atccagcgag catcataccc tggacccgct gaagatgccg gttcctggca
    16321 gtgcgccggc tacccgatcg ttcatggttg gccgcggggc gcgcggttac tccacgcagc
    16381 cgcgggcgtc cccgaacggg tcgcacggct tcttctgctt cttcggcggc ccatacggat
    16441 tgcccgacag ctcgccactg tcgaccggct tcttcgtggc cttgcccttc gggtgagcgg
    16501 cgaacgggtc gcggagctcg tcgtcgtagg gatcgacggg cgctgcttgc gccttttgct
    16561 tcgcggccgc ggccttgcgc agctggttct ggatctgacg ctccatcttc ggaaggacga
    16621 ccagcgtgcg cgcgacctgc tgcggcgtca ggatctttcg cagctcggcg aggcgcttca
    16681 cctcggtgtc ccacagcgcc ttttgattcg cggacgcgtc gtcgatcagc ttgtcgattg
    16741 ccttcgtatc cgtctgcgtg cccgccgctt cgagctgctt ctcgagatcg acgcgcgcga
    16801 cgagcagctt gtcgaattca tcgtcgtact tcgcgagcat cggaaacagc ttgccggccg
    16861 ttttctcgtc gagcgacagc tcttccgtga gcgtgtaagc gcgcagcgcg cggatccgct
    16921 tcttgatctt ctctcgacgt tcttgctgcg gtgcaggctg cgcccacgct ggtgtaacga
    16981 gcacgaaggc gagcgcgagt acgagccagc ggatcatgag cgttccttct cgagccattg
    17041 ttcgatctcg tcgagtgctg cgtcatcgag ctcgtcgagg ccgacgtcat cggtatcgag
    17101 cacctcgcgc gctgcttgat cgagattgtc gaggcttgcg tcgtcgacgt cgtcgaggtt
    17161 gagcgcttca ccatcgagcc acatcgcggg cgcattcgtg acttcgcgct gcgtgctcgg
    17221 cgtgtcgtgc tgcgatggtg acgtcagcgc atgctgagga gctggcgcgt cgtgatcgcg
    17281 catgacgatc aaagcgacag ccgccgtcgt cgcgagcgcc cacacgggga tgatccactt
    17341 ccagttccgc caccacggcc tcggtgcgcg gtcgccgact tcggcacgaa tcgccgcttc
    17401 gagcttctgc caattgggct cggttccctc cggcggcagc gcgcgaagct tcgcgatcag
    17461 ctcgtgatcg cgatcgtcga gctcgtcatc ctcacctggc cgacgcatgg tcacctccga
    17521 ggatgtcacg cagcttcttg acggcgtaat ggaagctgac cttcgcggtg ttctcgctgc
    17581 aatccgcgag ctcggcgact tccttgaacg acaggtcgtc gaacacgcgc agctcgagga
    17641 ccatgcgttg cttcggcggc agctgcgcga tcgctttgcg gagctgcgcg ctctcttgat
    17701 cgctcgcgat gcgacccggc gcgatcggga gctcggtgag cgagtcttcg gcgatctcgg
    17761 ttggctcttc gcggcgatga tcgcgcagcc acgacagcga gcagttgatc gcgatccgat
    17821 agagccagct gcgcacgctc gcggcaccgc gaaagccgcc cagcgccttg aatgcacgaa
    17881 cgaaaacgag ctgcgtgacg tccgcggcgt cggcatcgcg cttcacgtaa cggcggacga
    17941 tccgccagac acctttttga tgacgcctga caagcacgtc gaatgcgcta cggtcgccct
    18001 gccggaactt ctcggcgagc tcgcgatcgg gatcaatcgc gtcggtcacg cgagtgagcg
    18061 tggttgcgag gaccgggagc gccgccgtcg acacgtccta ttggaccccg cgcttgccac
    18121 gactgttaag agcaaggtag ggtccgcatc caactcacgg tcatcattga ggtaagccat
    18181 gctcgaccca gcccgaatgg acgacgtcct tcgccgagga ggcgatgttg ccacggtggt
    18241 gggctcgtgg aagagcctcg acgagcgccg gcgcaagctg caaggcgatc tcgacacgct
    18301 tcgccagcag cgcaacgccg ccaacgacaa gatgtcgaag ctcgacaaga agtcggacga
    18361 gttcaaggcc gccgttgccg agatgaaagg cgtctcgacg aagatcaaag aaggcgaagc
    18421 ggagctcggc accgtcgagc aagaagcgag cgcgctgctc ctgacgatcc cgaacgcgcc
    18481 gcacgagagc gttccgaacg gcacgagcga gcacgacaat gtcgagctgc acgtctgggg
    18541 caccaagccg acgttctcgt tcgcgccgaa gacgcacgcc gatctcggtc cgtcgctcgg
    18601 tctcgacttc gaagcgggcg cgaagatcac gggctctcgc ttcacggtgc tgcgtggcgc
    18661 ggcgtctcgc ctcacgcgcg gcctcatcaa ctacatgctc gatctgcaca cgcagaagca
    18721 tggctacggc gagatctggc caccggcgat cgtcaagcgc gcgtcgctgc gcggcaccgg
    18781 ccagctgccg aagttcgaag cggacttgtt caggctcgac attcccgtcg gcccggacca
    18841 cgtccccgat aacgatctgt tcctgtcgcc gaccgccgaa gtacaggtga cgaacctcta
    18901 caacgatcac atcttcgagg cgggcgagct gccggttcgc ctgaccgcat actcgccgtg
    18961 cttccgcgcc gaagccggcg ccggcggcaa agacacgcgc ggcctcatcc gccagcatca
    19021 gttcgacaag gtcgagctcg tgaagttcgt cgagcccgag cattcgtacg gcgagctcga
    19081 ggcactgcgc caagacgccg aagcggtact gcagggcctc ggccttcatt atcgggtgat
    19141 gacgctgtgc tcgggcgaca tgggctttgc gtccgcgaag acgtacgacc tcgaggtgtg
    19201 gctgcccggc caggacgcgt tccgcgagat ctccagctgc tcgaacttcg agtcgtttca
    19261 ggcgcgtcgc gcgggaatcc gctaccggac ggagccgaag gccaagccgc gcgcggtcca
    19321 cacgctcaac ggctcgggca tcgcaatcgg ccggacgatc gtcgcgatca tcgagcagta
    19381 ccagcaggcg gacggaaccg tcgtcatccc ggcggcgctc cgcccgtacg tcggcggcat
    19441 ggagcgcctg acgccggtct aggtccgcga gactagggaa aaaggtagat ccagctttac
    19501 atccctacat tgatccctat aaatacgccc tgcctctggg accgcctgga ggagtggccg
    19561 agcggtcgaa ggcagcggtc ttgaaaaccg ccgagcgcaa gctccggggg ttcgaatccc
    19621 tcctcctccg cagagttgat aatcgaatag tgtcacgcaa gtggtcatgg gcgggacgtg
    19681 ccccgtgtcg acgaaggcag cttcttcgac gagccgtccc gcaccacaca ccgttttggc
    19741 aagcccatct tggacagatg gccgagtggc tgaaggcacc ggtttgctaa accggcatat
    19801 ggggtttttc ctgtatcgag ggttcgaatc cctctctgtc cgcccgtaat ttgatcattt
    19861 ggatccgtag ctcaatggat agagcaccgg tctacggaac cgggggttag aggttcgacc
    19921 cctctcggat ccgcaacaag cagaaggcac atcgatgacg acaacagatg accaatggat
    19981 gcaggtcgcg ctcgaagaag cgcgagctgc agcagcggcg ggagatgttc ccgtcggtgc
    20041 ggtcatcgtc ggaagcgatg gtgcgctgct cggtcgcgga cgcaatcgac gcgagctcga
    20101 caaggatcca accgcgcatg cggagatcgt cgcactgcgc gcagcggcag caacactcgg
    20161 catgtggcga gtcgaagcaa cgctgttcgt cacgcaggag ccctgcccga tgtgcgcagg
    20221 cgcgctcgtc aacgcgcgag tcacccggct cgtctacggc tgcgcaaatc cgaaggccgg
    20281 cgcggtcgca tcgctcttct cgctcgtcac cgattctcga ctcaatcacc ggatggcggt
    20341 cacgtcgggt gttctcgcga cggaatgcgc agcggtgctc tcacaatttt ttgccgatct
    20401 tcgtcgaggt ccgacgtacg gcggctcgta aagtttctgg acaggtgtcc gagtggccga
    20461 tggtgtctga ttcgaaatca gatgtacgca agtaccgggg gttcaaatcc ctccctgtcc
    20521 gcaatttccg atggtcacca ccatcattgg agcggtgacc gagtggccga aggtgcacga
    20581 ttggaaatcg tgtgaactga aaggttccga gggttcgaat ccctcccgct ccgcaacctt
    20641 ctactgccga gagaagctgc ggcaccacac tgcgtgacgc ggaccattct tatggtcccg
    20701 gatgacgtcg aggctgtgaa ccccgccagg cccggaaggg agcaacggta gcggagtagg
    20761 cgggtgtctg gggccacctt ttagatgagc gactcaactc gagt
//
LOCUS       DATLBZ010000250        12337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4735827, whole genome shotgun sequence.
ACCESSION   DATLBZ010000250 DATLBZ010000000
VERSION     DATLBZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4735827"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VMZ53_13255"
     CDS             <1..382
                     /locus_tag="VMZ53_13255"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUS29472.1"
                     /translation="DVVCGYDELRVAAPDGVDIVLDGVGGQVFRDSLAVLKPRGVVVV
                     VGASSGEPQLIDPQKLVMRSHTVVGLHLRHLLANPDELAHTIDACRALDVTARVTAMP
                     MADIANAHARLQGREIAGKLVLTW"
     gene            complement(389..1108)
                     /locus_tag="VMZ53_13260"
     CDS             complement(389..1108)
                     /locus_tag="VMZ53_13260"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HUS29473.1"
                     /translation="MVVLAIVIGALAMLVCEQVRPARRWPRVETWWVRALAINGIQVA
                     AVFIAGATWERWIRGPHVLSVASLPWPAEVAVAYLAYAMWLYWWHRARHAIGPLWRWL
                     HQVHHSPARIEVLTAFYKHPLEIVADSVLASVVLFVVLGVSKESAFLASNVSGILGLF
                     YHWNIRTPRWLGYIVQRPESHCVHHERGVHAFNYSELPLIDMLFGTFRNPHTFDRECG
                     FGDERERKVAALLLGSDVTRS"
     gene            1133..1888
                     /locus_tag="VMZ53_13265"
     CDS             1133..1888
                     /locus_tag="VMZ53_13265"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HUS29474.1"
                     /translation="MNRVQKLLSNLPELLRRDAAGEPPLWAPADPLQYARFLIPDLTG
                     KPWHDAHDFAFTRAFEANHAAIRAELEAFLAQQRVTFRNYVGPILHEQADSGAWHVLY
                     LDYRGHLWDDHIKCFPTLWSVVQQVPRRSGTVFISRLTPGAHIPKHCGSTNTQLTCHF
                     GIVVPTGVEMRVAREVRQQTEGRCTIFDDSFEHEVWNRADANRYNVFMQFWHPELSDE
                     EIAGIQQLEKLPQIQNVITQYLEGASALAPVEN"
     gene            complement(1860..2444)
                     /locus_tag="VMZ53_13270"
     CDS             complement(1860..2444)
                     /locus_tag="VMZ53_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29475.1"
                     /translation="MKPLPSTLDVWMQRAGVLLLALLPLHVWLRPEGGWALLAACDIC
                     YIACALGLLFRSHRLVGAAFLFSLTVSLPAMTMGLATGTYPWNWSGIAIHIVPATFGG
                     VRVYREGLPRTAAFDAWFVYAAAVMVASVVSPPELNINFGARPWTPVAGTFSLRVFQT
                     LLIMTVGALLWLGQLVGWLISRRRAASSRPAPAR"
     gene            complement(2441..4426)
                     /locus_tag="VMZ53_13275"
     CDS             complement(2441..4426)
                     /locus_tag="VMZ53_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29476.1"
                     /translation="MKQLLARPAVVVVAALLAFALTLPSITNGLAIDDHLYRARVVDD
                     EWSAGRSARDLFVFANPDHASPSPAADERTQQIESGELSWWAAPKLRWGFMRPLPALI
                     HHAEWRALGRSDGGTMWMHLHSVLWMAALSAVVALLYRRLIGGLSGSTGATWIAGLAA
                     VLYAIDDAHGFAVGWLANRCGIQGAVFAVLALLAHDRWRRRARKAASASGATSATPTN
                     GTTTADGATRASGVTRVGTFAFVAPLLFLLSLLCSEQMVAAAGFLFAYALCLDDRRGR
                     FAALAPYALITVAWFVARSVLGYGSVGTGSYTDPFHEPATYALHVLERVPILVHSQLG
                     ALPADLWEVLFVRQGLTWVMVLAGVVYAALFALGVAKLVRADRVAKFWVVGGLLALGV
                     VCGAHPNDRHLLLVGIAGSGLVAQFCGAWLDRERAVLPRLAPVLGVFLVFVHAIAAPI
                     LLPVRARIPGSVARGVERIDQLVPGDAALAQQDLVLVNVPFKYLCNFASVVRRSNGGV
                     SPRRWRCLGVSPDDVVVARVDDRTISLTPTTATSAANATGTHVGYLRFFEDTNVRARG
                     IPFATGDRVDVPGLAITIREVTGDGRPATIEAHFDVPLEDASLRWLVWRDGAYRPFVP
                     PAIGESATIPAQHYAFGDLLSDDAAKPQAAPAKAASP"
     gene            complement(4436..5254)
                     /locus_tag="VMZ53_13280"
     CDS             complement(4436..5254)
                     /locus_tag="VMZ53_13280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HUS29477.1"
                     /translation="MPRSLLFTVPALLAAALLIVLELRWTRRHAEAKGYSLGDSLANG
                     ALGGLKVSFGALVKLGALPLFAFVYDHRIADLGRPDAWWSWLILLFAEDLCFYTFHRT
                     HHAVRILWAAHVSHHSSEHYNLSTAFRQTLLTTITSPIFWMPLALLGFSPWMILLAQV
                     WSQLYQFGLHTEAVGKLGPLEWIMNTPSHHRVHHGKQPQYIDRNHAGIFIIWDRLFGT
                     FEPERERVQYGLTRDIKTNNVGRILFHELVDITRDARSAPTLRGKLDAVFGRPR"
     gene            complement(5284..6750)
                     /locus_tag="VMZ53_13285"
     CDS             complement(5284..6750)
                     /locus_tag="VMZ53_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HUS29478.1"
                     /translation="MRRLAVLLTLTLAGACGGDSAPTQPDAPAPDVPVDTATTFPACA
                     EFSATGMAVPAHIVGSLSASDVQSPTSCTSIDAPFGVESAGPDAVVRLDGLVTGNPYI
                     VRLNSGDDLAFYVASGCSTPNGPASTECALFVDASYGAREVGRFVATAETEYVIVDYY
                     ASAPPDDASFALDVYAETCSTDAQCSGSMPACVAGRCVECDSSFDCSNAAKPACNRDT
                     NTCVVGTELCAPDDVGEPANDGPSGAIALVLDGTGLATISGDICQANTPLLEADFYKF
                     QVTTLGDTWDISLAWTGNRDLDLRVYDAQGESLGLSFWEHPETIRLTYLAVGTYYIRV
                     TNASPISATSLGYTLTAQRTAGAGCTARSQCAAEHRNQLFRGDCVAGACVPIDGAGAL
                     TEGNACDSVSDCASALSCPSFYFTANADTRDVCARSCTTDAGCSALGSNYVCSTYLDT
                     NMCVQKCTADDQCPTTFDQPSTGPWYRLHCDIPSGKCM"
     gene            complement(6871..8001)
                     /locus_tag="VMZ53_13290"
     CDS             complement(6871..8001)
                     /locus_tag="VMZ53_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HUS29479.1"
                     /translation="MSIVSRLTKLSVVAGERIGLFEPPPAALKPGEKPPRHTFRWDLD
                     KTYLRTEFDSLKDLAKSAIETAADKQAFPGATALLRALRQDGNRICIVSGSPTQMRQV
                     LAAKLALDGVEYDEFVLKNNLKNILKGRFRALRAQIPYKLPAMLASRIAAPGEAETLF
                     GDDAEADAIIYCLYADVVAGVVSLQDLERVLTASRAYEDDVERCLDLARRVPKAPIVR
                     RMFIHLDRRSPPMGFRRFGPRLVPVFNYFQAALVLYADKVLTARQVIFVALEMLDSGQ
                     FELGHLATSVQDVIRRGRITRDKALELVAEANEAAKSGALSGRSDLPPFEQIAESFRV
                     RVRELGAQPVNEPPPDEEPLDYVRLVDEEHEHRHRRRRSGMG"
     gene            complement(8004..9071)
                     /gene="plsX"
                     /locus_tag="VMZ53_13295"
     CDS             complement(8004..9071)
                     /gene="plsX"
                     /locus_tag="VMZ53_13295"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HUS29480.1"
                     /translation="MHRIVVDAMGGDHAPDEVVRGAAEASLSLNSAEIILVGDAAVLG
                     RLLPRMRHDGARVRVHHAPTFVEMDEKPAEALAAKPEASIVVAADLVARGEGDALVSA
                     GNTGASVLACARRWQLLQGVRRAALAAVYPTELRRGEKDDPFSLILDVGATIEASAED
                     LVGFAVMGSAYAKLVSQNRRPRVALLSNGTEAGKGPTAVVDAHARLVETTELNFIGNI
                     EGLDIPRGVADVVVTSGFVGNVVLKMLEGVSETVVRLARYAHKERLAWRLGLVALSSA
                     IDQLKEVTDWQQYGGAPLLGFTHPFIKAHGRSNARAVQNAVKVAHKALAGNLCGNIAR
                     TMAELDERAQRRASSQSDAAG"
     gene            9104..9955
                     /locus_tag="VMZ53_13300"
     CDS             9104..9955
                     /locus_tag="VMZ53_13300"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HUS29481.1"
                     /translation="MSENRRELEDSIHVTLQKGATYGDYLKLDEVLRAQQPVTGVHDE
                     MLFIIQHQTSELWIKLLLHELDRAVELVRTDRLEESFKIFARVGHIQRMLFEQWAVLE
                     TMTPSEYMQFRDALGKSSGFQSYQYRALEFVLGNKDAATLRPHAHDKKIHGELERRLS
                     QPSLYDEFLRWLARRGHAIPEDRVNRDFTLPYEKSDLVTEAFRVIYAEPERHYAAYDM
                     AEKLVDVEERFQLWRFRHMTTVKRIIGYAPGTGGSSGVGFLKRALDLTFFPELWDVRT
                     HLASPKK"
     gene            9952..>12337
                     /gene="polA"
                     /locus_tag="VMZ53_13305"
     CDS             9952..>12337
                     /gene="polA"
                     /locus_tag="VMZ53_13305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HUS29482.1"
                     /translation="MKERLYIVDGHGYIFRAHYGLMNVSRGERREVKLSTAEGMPTGA
                     LYVFSRMLLRLEEDNQPKRMAVVFDHKGGRNFRAEMYADYKAHRKEAPEELTVQMEYF
                     PKIVNGLGWPCLAIPGVEADDVIATLVEKAKEKDWEVVIFSADKDIMQLIGPDVTMID
                     ALHQKVYTREEVIKKMGVPPEKIPDFLALVGDTSDNIPGIKGVGDKTAAGLLEKYGTL
                     DALIAENPVVPRLSVKQPFSDPEQLERVTVSRKLVELKRDVEVPELDSLVVGDWDLPG
                     LLQLFTELEFNLLVEKVKMKMPPSDDMVVVAAPEASPMPTVETRQDTRVVVRPDVIAE
                     VAAAAERAGKMAITVELDPERIEKARMVAVVIAVPGEPPAYVPLGHRYIGAPAPPPAT
                     DLKPLYQVLEDPTVAKICHDAKTVARAFLDVGVVVAGVVEDTMLAAFLLDPTQEAEPG
                     ETVAQRLGGVLLPARATIAGKAKVSLEAVEVERAAPWAGAITHSLLPISEQLPARLET
                     GGLAKLYREIELPTALLLAQVERIGIHIDTAHFKKLANEVSTKIEQLEKQIFELAGTE
                     FNVGSPKQLGEILFEKLGLATKGVRRTKTGWSTEAETLEQLDHPIVAPIMEHREISKL
                     KGTYLDALPPLVSARTGRVHTKFNQVVAETGRISSQDPNLQNIPIRSDLGMQIRKGFI
                     AAPGKVLIAADYSQIELRILAHLSGDPVLSSAFRDRVDVHTQTAAEVFSVAREEVTPE
                     QRRIAKAVNYGLSYGQSDFGLARTLDITRTQAAEYSRRYFQRFPSIHKFMDEIVA"
BASE COUNT         2236 a         4005 c         4162 g         1934 t
ORIGIN      
        1 agatgtcgtg tgcggctatg acgagctgcg cgtggcggca cccgatggtg tggacatcgt
       61 gctcgatggc gtcggcgggc aggtctttcg cgacagcctc gcggtgctga agccgcgggg
      121 cgtggtcgtc gtcgtcggtg cgtcgagcgg cgagccccag ctgatcgatc cacagaagct
      181 cgtcatgcga tcgcacacgg tcgtcggctt gcatctgcgg cacttgctcg cgaatcccga
      241 cgagctcgcg cacacgatcg atgcgtgtcg agcgctggac gtcacggcgc gcgtcacggc
      301 gatgccgatg gcggacatcg cgaacgcgca cgcacgcctg cagggacgcg agatcgccgg
      361 caagctcgtg ttgacctggt agcgggcgct acgagcgcgt cacgtcgctg ccgagcagga
      421 gcgctgcgac cttgcgctcg cgctcgtcgc cgaagccgca ttcgcgatcg aacgtgtgcg
      481 ggttgcggaa cgtgccgaac agcatgtcga tgagcggcag ctcggagtag ttgaatgcgt
      541 gcacgccacg ctcgtggtga acacagtgcg actccgggcg ttggacgatg tagccgagcc
      601 agcgcggcgt tcgaatgttc cagtgataga agagcccgag gatgccgctc acgttcgacg
      661 cgaggaaggc gctctccttc gagacgccga gcacgacgaa cagcacgacg gacgcgagca
      721 ccgagtcggc gacgatctcg agcgggtgct tgtagaacgc ggtcagcacc tcgatgcgcg
      781 cggggctgtg atggacctgg tggagccacc gccacagcgg cccgatcgcg tgccgcgcgc
      841 ggtgccacca atacagccac atcgcgtaag cgaggtaggc gacggcgact tccgccggcc
      901 atggcagcga cgcgaccgag aggacgtgag ggccgcgaat ccaacgctcc caggtcgcac
      961 cggcgatgaa gacggccgcc acttgaatgc cgttgattgc caacgcgcgc acccaccacg
     1021 tttcgacgcg cggccaccgt cgagccggac gcacttgctc gcacacgagc atcgcgagtg
     1081 cgccgatcac gatggcgagg accaccacgc tcgtgttgta acatcccgcg cgatgaacag
     1141 agtgcagaag ctcctctcga acttgcccga gctcctgcga cgcgacgctg ccggagagcc
     1201 gccactgtgg gcgcccgccg atccgctgca gtacgcgcgg ttcctcatcc cggatctaac
     1261 tggcaagccg tggcacgacg cgcatgactt cgcgtttacg cgcgccttcg aagcgaacca
     1321 cgccgcgatt cgcgccgagc tcgaagcgtt tctcgcgcag cagcgagtga cgtttcgcaa
     1381 ctacgtcggg ccgatcctgc acgagcaggc ggactccggc gcgtggcacg tcctctatct
     1441 cgattatcgt ggccatcttt gggacgacca catcaagtgc ttcccgacgc tgtggagcgt
     1501 ggtgcagcag gtgccgcgtc gctcgggcac cgtgttcatc tcgcggctca cgcccggcgc
     1561 gcacatcccg aagcactgcg gctcgacgaa cacgcagctc acctgtcact tcggcatcgt
     1621 cgtgccgacc ggcgtcgaga tgcgcgtcgc gcgcgaggtc cgtcaacaga ccgaaggtcg
     1681 ctgcacgatc ttcgacgatt cgttcgagca cgaggtctgg aaccgcgccg acgcgaaccg
     1741 ctacaacgtg ttcatgcagt tctggcatcc cgagctctcc gacgaggaga tcgcgggcat
     1801 ccagcagctc gagaagctgc cgcagatcca gaacgtgatc acgcagtacc tcgaaggtgc
     1861 tagcgcgctg gcgccggtcg agaactagcc gcgcgtcgtc gcgagatgag ccacccgacg
     1921 agctgaccga gccagagcag cgcgccgact gtcatgatca gcagcgtctg aaagacgcgg
     1981 agcgagaacg ttccggcgac cggcgtccaa ggccgcgcac cgaagttgat gttgagctcg
     2041 ggtggggaga caacggacgc caccatcacg gcagctgcgt acacgaacca cgcatcgaaa
     2101 gcggcggtcc gcgggaggcc ttcgcgatag acgcgcacgc cgccgaatgt cgcgggcacg
     2161 atatggatcg caatgccgct ccagttccac ggatacgtcc ccgtcgcgag gcccatcgtc
     2221 atggcgggga gactgacggt gagcgagaac aagaacgccg cgccgacgag ccgatgcgat
     2281 cgaaatagga ggccgagcgc gcacgcgatg taacagatgt cgcacgccgc caagagcgcc
     2341 caaccgcctt cgggtcgaag ccacacgtgg agtggcaaga gcgcgagcag caacacgccc
     2401 gcgcgctgca tccagacatc gagcgtgctc ggcagcggct tcacgggctc gccgccttcg
     2461 ccggtgccgc ctgcggcttc gccgcgtcgt cggagagcag atcgccgaat gcgtagtgct
     2521 gcgcgggaat ggtcgcgctc tctccgatcg cgggcggcac gaacggacga tacgcgccat
     2581 cgcgccagac aagccagcgc aacgatgcgt cctcgagcgg tacatcgaag tgcgcctcga
     2641 tcgtcgccgg ccgcccgtcg ccggtcactt cgcggatggt gatggcaaga cccggcacat
     2701 cgacgcggtc acccgtcgca aagggaatgc cgcgcgcgcg cacgttcgtg tcctcgaaga
     2761 accgcagata acccacgtgg gtgcccgtgg cattcgcagc gctcgtggcg gtggtcggcg
     2821 tcaacgaaat tgttcgatca tcgacgcgcg cgaccacgac gtcgtcgggc gacacgccca
     2881 ggcatcgcca gcgccgcggc gacacgccgc cattggaccg ccgcacgacg ctcgcaaaat
     2941 tgcacaggta cttgaacggt acgttgacga gcaccaagtc ctgctgcgcg agcgcggcgt
     3001 cgccggggac gagctgatcg atgcgctcga caccacgcgc cacggatccc ggaatgcgcg
     3061 cgcgcacggg caacaagatc ggcgctgcga tcgcgtgaac gaatacgagg aacacgccca
     3121 acaccggcgc gagcctcggc aacaccgcac gctcgcgatc cagccacgcg ccgcaaaact
     3181 gcgcgacgag tcccgagccc gcgatcccga cgagcagcaa atggcgatcg ttggggtgcg
     3241 cgccgcacac gacgccgagg gcaagcaagc cgcccaccac ccagaacttc gcgacgcggt
     3301 ccgcgcggac gagcttcgca acgccgagcg cgaagagcgc cgcatacacg acgcccgcga
     3361 gcaccatgac ccacgtcagc ccctggcgaa cgaacaggac ctcccacaga tccgcgggca
     3421 acgcaccgag ctggctgtgg acgagaatcg gcacgcgctc gagcacgtgc agcgcatacg
     3481 tggcgggctc gtgaaacggg tcggtatacg agccggtccc gacgctgccg tacccgagca
     3541 cgctgcgcgc gacaaaccac gcgacggtga tgagcgcata cggcgcgagt gccgcgaaac
     3601 gcccgcgccg atcatcaagg cacagtgcgt acgcaaacag aaagcccgcg gccgcgacca
     3661 tttgctccga gcacagcagg gagagcagga acaggagagg ggcgacgaac gcgaaggtac
     3721 caactctcgt cacgccgctt gccctcgtcg cgccgtccgc cgtcgtcgtg ccgttcgtcg
     3781 gcgtcgcgct tgtcgcgccg cttgcgctgg ccgccttgcg agctcgtcgt cgccatcgat
     3841 cgtgggcgag cagcgcgagc accgcgaaca ccgcaccctg gattccgcag cgattcgcga
     3901 gccagccgac cgcgaagccg tgcgcatcgt cgatcgcgta gagcacggcg gcgagccccg
     3961 cgatccaggt cgcgcctgtc gagccgctga ggccgccgat cagccgccgg tacagcagcg
     4021 cgacaaccgc ggagagcgcc gccatccaca gcacgctgtg taagtgcatc cacatcgtgc
     4081 cgccgtcgct gcggccgagc gcgcgccact ccgcgtggtg aatcagcgcc ggcagcggcc
     4141 gcatgaagcc ccaccgcagc ttcggcgcgg cccaccacga cagctcgccg ctctcgatct
     4201 gctgcgtgcg ctcgtcggca gcgggcgacg gcgatgcgtg atcgggattc gcgaacacga
     4261 acaggtcgcg cgcggagcgc ccggctgacc actcgtcgtc gacgacacgt gcgcggtaga
     4321 ggtggtcgtc gattgcgagg ccgttcgtga tcgacggcag cgtcagcgcg aatgcgagca
     4381 gcgccgcgac tacaacgacg gcgggcctcg cgaggagctg cttcacgcgc accggctacc
     4441 gagggcggcc gaacacggcg tcgagctttc cgcgcagcgt cggggcggaa cgcgcgtcgc
     4501 gcgtgatgtc gacgagctcg tggaacagga tgcgtccgac gttgttcgtc ttgatgtcgc
     4561 gggtgaggcc gtattgcacc cgttcgcgtt cgggctcgaa cgtgccgaac aggcgatccc
     4621 agatgatgaa gatgcccgcg tgatttcgat cgatgtactg cggctgcttg ccgtggtgca
     4681 cgcggtggtg cgacggcgtg ttcatgatcc attcgagcgg gcccagcttg ccgaccgctt
     4741 cggtgtggag gccgaattgg tagagctggc tccagacttg cgccagcagg atcatccacg
     4801 gcgagaagcc gagcagcgcg agcggcatcc agaagatcgg cgacgtgatc gtggtgagca
     4861 gcgtttggcg gaacgccgtc gagaggttgt agtgctcgct cgagtgatgc gagacgtgcg
     4921 cggcccacag gatgcgcacg gcgtggtgcg tgcggtggaa cgtgtagaag cagaggtctt
     4981 cggcgaacag gaggatgagc cacgaccacc acgcgtcggg gcggcccaga tcggcgatgc
     5041 ggtggtcgta gacgaacgcg aacaggggga gtgcgccgag cttgacgagc gcgccgaagc
     5101 tgaccttgag cccgccgagc gcgccgttcg cgagcgagtc gccgagcgag tagccttttg
     5161 cttcggcgtg gcgacgcgtc caccgcagct cgagcacgat cagcagcgca gcggcgagca
     5221 gcgcggggac cgtgaagagc agcgagcggg gcacgggacc attctacgcg cgtgcggccg
     5281 cagctacatg catttgccgg atgggatgtc gcagtgcagt cggtaccagg ggcccgtcga
     5341 cggctggtcg aacgtcgtcg ggcactgatc gtccgccgtg catttttgga cgcacatgtt
     5401 cgtgtcgaga tacgtgctgc agacgtagtt gctgccgagc gcgctgcagc cggcgtcggt
     5461 cgtgcagctg cgagcgcaca catcgcgcgt gtcggcgttt gccgtgaagt agaaggacgg
     5521 gcaggagagg gcggacgcgc agtccgagac gctgtcgcac gcgttgcctt cggtgagagc
     5581 accggcgcca tcgatgggga cgcatgcgcc cgcgacgcag tcaccgcgga agagctggtt
     5641 gcggtgctcg gccgcgcatt gcgaacgcgc cgtgcagcct gcgcctgcgg tccgttgcgc
     5701 ggtgagcgtg tagccgagcg acgtcgctga aatgggcgac gcgttggtca cgcggatgta
     5761 gtacgtgccg acggcgaggt acgtgaggcg gatggtttcg ggatgctccc agaacgagag
     5821 gccgagcgat tcgccttgtg cgtcgtagac gcgcagatcg aggtcgcgat tgccggtcca
     5881 cgcgagcgag atgtcccacg tgtcgccgag cgtcgtgact tgaaacttgt agaagtcggc
     5941 ttcgagcagc ggcgtgtttg cctggcagat gtcgccgctg atcgtcgcga ggccggtgcc
     6001 gtcgaggacg agcgcgattg cgccggacgg gccatcgttg gcgggttcgc cgacatcgtc
     6061 gggcgcacag agctcggtgc ctaccacgca cgtgttcgtg tcgcgattgc acgcgggctt
     6121 tgcggcgttg ctgcaatcga agctcgagtc gcattccacg catcgaccgg cgacgcacgc
     6181 gggcatagag ccgctgcact gcgcgtcagt cgagcaggtc tcggcgtaga catcgagcgc
     6241 gaacgacgcg tcgtccggag gtgccgatgc gtagtagtcg acgatgacgt actcggtctc
     6301 ggcggtggca acgaagcgcc cgacttcacg cgcgccgtag ctcgcatcga cgaacagagc
     6361 gcactcggtg gatgcgggac cgttcggcgt cgagcaaccg gacgcgacgt agaacgcgag
     6421 gtcgtcgccc gaattgaggc gcacgatgta cgggttgcct gtcacgaggc catcgaggcg
     6481 gacgactgcg tccggccccg ccgactccac gccgaacggc gcgtcgatcg acgtacacga
     6541 cgtcgggctc tgcacgtccg atgcggagag cgagcccacg atatgcgctg gaaccgccat
     6601 gcccgttgcg ctgaactcgg cgcatgcggg aaacgtcgtc gcggtgtcga ccggcacatc
     6661 cggcgccggc gcatcgggtt gggtcggcgc agaatcgcca ccgcaggcac ccgcgagggt
     6721 gagcgtcagc aagaccgcga gtcggcgcac gaaaccacac tacaaaaccg acgccggcaa
     6781 agcgaaacgt tggtcctcac atggggtgat ctagctgcga tgcgctgacc tcaacgcgga
     6841 actgtcgctg tcgcgcggtg tcgcggccgc ttagcccatg ccggagcgac gccgccgatg
     6901 tcggtgttcg tgctcctcgt cgaccaggcg cacgtaatcg agcggctctt cgtcgggcgg
     6961 cggctcgttc acaggctgag ccccaagctc gcggactcgc acgcgaaacg attctgcgat
     7021 ctgctcgaac ggcggcaagt cgctgcgacc agagagcgcg cccgattttg ccgcctcgtt
     7081 tgcttcggcc acgagctcga gcgccttgtc gcgtgtgatc cgaccgcgcc ggatcacatc
     7141 ctgaacactc gttgcgaggt ggccgagctc gaattgtccc gagtcgagca tctcgagtgc
     7201 gacgaagatc acctgacgcg cggtgaggac tttgtcggcg tacagaacga gcgctgcctg
     7261 gaaatagttg aagaccggaa cgaggcgcgg accgaaccgg cggaagccca tcggcggcga
     7321 gcggcgatcg agatggatga acatgcggcg aacgatcggc gccttcggca cgcgacgcgc
     7381 gagatcaagg cagcgctcga cgtcgtcttc atacgcacgt gacgcggtga ggacgcgttc
     7441 gagatcttgg agcgacacga cgcccgcaac gacatcggcg tagaggcagt agatgatcgc
     7501 atcggcttcg gcgtcgtcgc cgaatagcgt ctcggcttcg ccgggcgcgg caatgcgtga
     7561 cgcgagcatc gcgggcagct tgtagggaat ctgtgcgcgc agcgcgcgga agcggccctt
     7621 caggatgttc ttcagattgt tcttgaggac gaactcatcg tactcgacgc cgtcgagcgc
     7681 gagttttgcc gcgagcacct ggcgcatttg cgtcggactt cccgacacga tgcagatgcg
     7741 gttgccgtct tggcgaagcg cacgaagcag cgcggtcgcg ccaggaaacg cctgcttatc
     7801 agcggcagtt tcgatcgcgc tcttcgcgag atccttcagc gagtcgaact cggtgcgcag
     7861 atacgtcttg tcgagatccc agcggaacgt gtggcgcggt ggtttctcgc cgggcttgag
     7921 cgcggcaggt ggtggctcga acagaccgat gcgctcgccg gcaacgaccg agagcttcgt
     7981 gaggcgactg acgatcgaca tgattagccg gctgcgtccg actggctgct ggcgcgacgc
     8041 tgcgcgcgtt cgtcgagctc ggccatcgtg cgcgcgatgt tgccgcagag atttccggcg
     8101 agtgccttgt gcgcgacctt cacggcgttc tgcaccgccc gcgcgttcga gcggccatgc
     8161 gctttgatga acggatgcgt gaagccgagg agtggcgcgc cgccgtactg ctgccagtcc
     8221 gtgacttcct tcagctgatc gatcgccgac gacagtgcga cgagcccgag gcgccacgcg
     8281 agccgctctt tgtgcgcgta ccgcgcgagc cgaacgacgg tctcgctgac accttcgagc
     8341 atcttcagca cgacgttgcc gacgaagccg ctcgtgacga cgacatctgc gacgccgcgc
     8401 gggatatcga gaccttcgat gttgccgatg aagttcagct ccgtcgtctc gacgagccgg
     8461 gcgtgcgcat cgacgacggc cgtcgggccc ttgcccgctt cggtgccgtt cgacagcagc
     8521 gcgacgcgcg gccgccggtt ctgcgagacg agctttgcgt aggcagagcc catcacggca
     8581 aagccgacga gatcctcggc gctcgcttcg atcgtcgccc cgacgtcgag gatgagcgag
     8641 aacggatcgt ctttctcgcc gcgccgtagc tcggtcgggt agacggccgc gagtgccgca
     8701 cgccgcacgc cttggagcag ctgccaccgt cgcgcgcacg cgaggacgct cgctcccgtg
     8761 ttgccggccg agacgagcgc atcgccttcg ccgcgagcga cgagatcggc agcgacgacg
     8821 atcgaggcct cgggcttcgc agcgagtgcc tctgcgggtt tttcgtccat ttcgacgaac
     8881 gtcggcgcgt ggtgaacgcg tacgcgcgcg ccatcgtggc gcatgcgggg caggagcctg
     8941 ccgagcaccg ccgcatctcc gacgaggatg atttccgcgg agttgagcga tagcgaggcc
     9001 tcggccgcac cccgaacgac ctcgtccggt gcgtgatcgc ctcccatcgc gtcgacgacg
     9061 atgcggtgca ttgcggggcc atattgccat aagctgcccg cagatgagtg agaaccgccg
     9121 cgagttagaa gactccattc acgtcacgct ccagaagggc gcgacctacg gcgactacct
     9181 caagctcgac gaggttctca gagcgcagca gccggtcacc ggcgtccacg acgagatgct
     9241 gttcatcatt cagcatcaaa cgagcgagct gtggatcaag ttgctgctgc acgagctcga
     9301 tcgcgccgtg gagctggttc gcaccgatcg cctcgaggag tcgttcaaga tcttcgctcg
     9361 cgtcggtcac atccaacgaa tgctcttcga gcagtgggcc gtcctcgaga cgatgacgcc
     9421 gagcgagtac atgcagtttc gcgacgcgct cggtaaatcg agtggctttc agagctatca
     9481 gtatcgcgcc ctcgagttcg tgctcggcaa caaggacgcc gcgacgctgc ggccgcacgc
     9541 gcacgacaag aagatccacg gcgagctcga gcgtcgcctc tcgcagccgt cgctctacga
     9601 cgagttcttg cgctggctgg cacgccgcgg tcatgcgatc cccgaggatc gcgtcaatcg
     9661 cgacttcacg ctgccgtacg agaagagcga tctcgtgacc gaagcattcc gcgtgatcta
     9721 cgccgagccc gagcgtcact acgcggcgta cgacatggcg gagaagctcg tcgacgtcga
     9781 ggagcggttt cagctctggc gcttccgcca catgacgacg gtcaaacgca tcatcggtta
     9841 tgcgccagga acgggtggaa gctctggcgt cggcttcttg aaacgggcac tcgatctgac
     9901 attcttcccc gagctctggg atgtgcgaac gcacctcgct tcgccgaaga aatgaaagaa
     9961 cgcctctaca tcgtcgacgg gcacggatac atcttccgcg cccactacgg cctcatgaac
    10021 gtctcgcgtg gcgagcgtcg cgaggtgaag ctgtcgacgg ccgagggcat gccgaccggg
    10081 gcgctctacg tgttctcccg aatgctgctg cgcctggagg aagacaacca gcccaaacgc
    10141 atggctgtcg tcttcgatca caaaggcggc cgcaactttc gcgcggagat gtacgccgac
    10201 tacaaggcgc accgcaaaga ggcaccggaa gagctcacgg tgcagatgga atacttcccg
    10261 aagatcgtga acggcctcgg ctggccatgc ctcgcgatcc ccggcgtcga agccgatgac
    10321 gtgatcgcga cgctcgtcga gaaagcgaag gagaaggact gggaggtcgt catcttctcc
    10381 gccgacaaag acatcatgca gctcatcggt cccgacgtga cgatgatcga tgcgctccat
    10441 cagaaggtgt acacgcgcga agaagtcatc aagaagatgg gcgtgccgcc cgagaagatt
    10501 ccagacttcc tcgcactcgt cggcgatacg tccgacaaca ttccgggcat caagggcgtc
    10561 ggcgacaaga cggcggcggg cctgctcgag aaatacggca cgctcgacgc actcattgcc
    10621 gagaatccgg tcgtgccgcg gctgagcgtc aagcagccgt tcagcgatcc cgagcagctc
    10681 gagcgcgtca ccgtttcgcg caagctcgtc gagctgaagc gcgacgtcga agtgccggag
    10741 ctcgactcgc tcgtcgtcgg cgactgggac ttgcccgggt tgctgcagct gttcaccgag
    10801 ctcgagttca acctgctcgt cgagaaggtg aagatgaaga tgccgccgtc ggacgacatg
    10861 gtcgtcgtcg cggcgcccga ggcctcgccg atgccgaccg tcgagacgcg gcaggacacg
    10921 cgcgtggtcg tgcggcccga cgtgatcgcc gaggtggcag cggcggccga gcgggcaggg
    10981 aagatggcga tcaccgtcga gctggatccc gagcggatcg agaaggcgcg catggtcgcg
    11041 gtggtgatcg ccgtgccggg cgaaccgccc gcatacgttc cgctcggtca tcgctatatc
    11101 ggcgcgcccg cgccgccgcc tgctaccgat ctcaagccgc tctatcaagt cctcgaggat
    11161 ccgacggtcg cgaagatctg ccacgacgcg aagaccgtgg cgcgcgcctt cctcgacgtt
    11221 ggcgttgtcg tcgcaggtgt cgtcgaagac acgatgctcg cggcgttcct cctcgatccg
    11281 acgcaagaag cggagcccgg cgagaccgtc gcgcagcgtc tcggtggcgt gctgcttccg
    11341 gcgcgcgcga cgatcgcggg caaggcgaag gtctcgctcg aggccgtcga ggtcgagcgt
    11401 gcggcgccgt gggccggtgc gatcacgcac tcgttgttgc cgattagcga gcagctgcca
    11461 gcgcgtctcg agacgggcgg gctcgccaag ctctatcgcg agatcgagct gccgaccgcg
    11521 ctgttgctcg cgcaggtcga acgcatcggt attcacatcg acaccgcgca cttcaagaag
    11581 ctcgcgaacg aggtctcgac gaagatcgag cagctcgaga agcagatctt cgagctcgcc
    11641 ggtaccgagt tcaacgtcgg ctcgccaaag cagctcggcg agatcctgtt cgagaagctc
    11701 ggcctcgcga cgaagggcgt gcgtcgcacg aagacggggt ggtcgaccga ggccgagacc
    11761 ctcgagcagc tcgaccatcc gatcgtcgct ccgatcatgg agcaccgcga gatcagcaag
    11821 ctcaagggca cgtacctcga cgcgctgccg ccgctcgtgt cggcgcgcac cggccgcgtc
    11881 catacgaagt tcaaccaagt cgtggccgag acgggccgta tctcgtcgca ggatccgaat
    11941 ctccagaaca tcccgatccg ctccgacctc ggcatgcaga tccgcaaggg cttcatcgcc
    12001 gcgcccggca aggtgctcat cgcagcggac tactcgcaga tcgagctgcg catcctcgcg
    12061 catttgtcgg gcgaccccgt gctgtcgtcg gcattccgcg atcgcgtcga cgttcacacg
    12121 cagaccgcgg ccgaggtgtt tagcgtcgcg cgcgaagagg taacgcccga gcagcgccgc
    12181 atcgcgaagg cggtgaacta cgggctgtcg tatggccaga gcgacttcgg cctcgcacgc
    12241 acgctcgaca tcacgcgaac gcaggcggcc gagtactcgc gtcgctactt ccagcgcttc
    12301 ccgtcgatcc acaagttcat ggacgagatc gtcgcgg
//
LOCUS       DATLBZ010000251         7193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9209007, whole genome shotgun sequence.
ACCESSION   DATLBZ010000251 DATLBZ010000000
VERSION     DATLBZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9209007"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VMZ53_13310"
     CDS             <1..1319
                     /locus_tag="VMZ53_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS29483.1"
                     /translation="RNHAKVTRDPETGRYTISDLQSSNGVRVNGQDYGKVELRRGDTV
                     DLGHVRLRFVEPGEDFVFNSSMVADIPETGSKRGLLVAIILGVLVLGGVGAFFFLNKK
                     DTTQTAGGQKTDLGSQKGSGDVVADNGNGGGSEIAVNPVTTDGSGSETNTMVKPPPDD
                     VAGNLALECKGYQAEAKWTDLSRCGEKLAKLDATEGKKFGATAASEIKAEGTLADLRK
                     EKDFAKAKRQSDAISKDSYYRKDADARIEELKAHDVGQAVSKAEAMKSKGQCDQIARL
                     ANDIKDKYGPEWGQRVAEQKQGCVESTGGNTGTTTVVQKDCSTRLVDNSKSCKTQFCK
                     ANPNDSNCATGQTTVTAPANCDAEALKDKGMENVNMGQHAAALAQFEASLRCKKDDYV
                     LALAFMAACNAQNAAKAKSYYGKLNPSAQTKYKVMCIRNHIDPAVP"
     gene            1466..3349
                     /locus_tag="VMZ53_13315"
     CDS             1466..3349
                     /locus_tag="VMZ53_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS29484.1"
                     /translation="MRVLGSAALMLALVGSTAHAQNAGGDVPLDTFRPAMDSRGYLTV
                     NASQVLGHKELSFGLGSLDWGYKMLNLEGGGSTYDVNNIVTATLIAAFGLKAGPAELE
                     FGASVPFVILFGDRGPDNLGDPNNANDDQFYKLDGQGLGNIGLHFKTRFLKTSRPPHL
                     GIGVIASIYLPTASPDSRWLGDKKPIPQVIGIIDKEFGSRGQLRLAANAGIRIRPDTT
                     WTNNDPTDGKMPAPNPPVPTTGASVTAGTEIPFGVGVAYAISVQKFDVVGEVIGAVPV
                     AGNENYQPLEAIGGIKLYLARNSFLSLGGGAGLLRKKETGANPDVRAFIGIVFEPNIG
                     DRDGDGIKDDIDKCPDDPEDYDRFEDEDGCPEPDNDRDGILDEDDKCPNIPEDKDGFQ
                     DEDGCPEGNKNDRDGDGILDDVDKCPDDPEDFDQFQDEDGCPDPDNDQDGILDVDDLC
                     PNDPEDKDGFEDEDGCPDLDNDKDRILDKDDKCPNEPETYNGFEDEDGCPDRGRVVVT
                     DTSIEILDMVYFEYNKAIIKSESFPILDAVAATLQGNPSISLVEIQGHTDERGDDAYN
                     LDLSDRRAASVKKYLIDKGVEEKRLESQGYGETQPLIREHNEKAWAKNRRVAFLILKR
                     ATD"
     gene            3441..3707
                     /locus_tag="VMZ53_13320"
     CDS             3441..3707
                     /locus_tag="VMZ53_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29485.1"
                     /translation="MIRGLLWLVAIVAFIWFGATVKLGHRTFFGHVRAIWHTDEAQDL
                     KHGVQEKAGPAVHKLEKGVKAGINAMKDEGSNGSGAGSAAPVHP"
     gene            3710..3925
                     /locus_tag="VMZ53_13325"
     CDS             3710..3925
                     /locus_tag="VMZ53_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29486.1"
                     /translation="MKFPLTQQLIDEAKRFELRSACRDCFYFDAAKDVCRHDWPDEGQ
                     RRWPLDAKDSNGNLPTDVAFCKEFELK"
     gene            3982..4938
                     /locus_tag="VMZ53_13330"
     CDS             3982..4938
                     /locus_tag="VMZ53_13330"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HUS29487.1"
                     /translation="MLDLVLSPEVAEARVDRRAVVALESTLVAHGLPYPQNLEVAQEL
                     QQAVRDAGAIPATIAIVDGRPHIGLTDALLERLAKDGKTFAKAGSTDLAVHMTRGKSA
                     ATTVSATAMLAARAKIKVFATGGIGGVHRGDAGDVSHDLVALAKTPIAVISAGAKAIL
                     DLPRTLETLETLGVLVVGYRTDEMPAFYTVRSGLRLEHFAGTAADLSAILKMHWEELG
                     GGGVLVANPIPSEASLDERLISDAIATSLLEASAAGITGKALTPFLLARLADVTGGAS
                     VRANRALALHNAKVGAELAIALNPHPIEGALTQRALLSRMCN"
     gene            complement(4957..6153)
                     /locus_tag="VMZ53_13335"
     CDS             complement(4957..6153)
                     /locus_tag="VMZ53_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29488.1"
                     /translation="MAAHADKAAPTPPTAPTTTPPTPVTKGAALPQTVGANEIMRLTP
                     PAGFVDDVVAYENQRIAYIVADTSTKAELHVVQLGCAKCAEEKKEIVVDLSPVTLRPI
                     SLRFVGQGRIFVVGATEEGKQVGALVELAKSPAAPVYKVGPAAHINVMKRDSAQRVVA
                     HFADPTKKGVKHHVEVFVLESGKRLITSKPLELEGAHDKRIDLTVNHWADGWTRAIGT
                     KGGEWVKKENQRSPDTEAVYDVVAGKYIENKPIGDLFEQRKRFQVLADANGQAEFLRM
                     TPDNQSIQVWRGGAMKTVDLDQPLASYDPKSMQGLVNADGTAWIVLKVDPVNADAVAR
                     KKADPEYLDIFKLGADGKATRKARILAKGVRHRFGVVGDFFWLLERSTGFDRGGKALI
                     VYSLAS"
     gene            6345..6680
                     /locus_tag="VMZ53_13340"
     CDS             6345..6680
                     /locus_tag="VMZ53_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HUS29489.1"
                     /translation="MSDDKIDLPQGTLELLVLKTLGLGAQHGWAISERIKQMSRDALQ
                     VQQGSLYPALHRMERQGWISAKWRASENNRKAKYYELTAAGRRRLDATTQEWRRLIAA
                     VQLVLDGGS"
     gene            6780..>7193
                     /locus_tag="VMZ53_13345"
     CDS             6780..>7193
                     /locus_tag="VMZ53_13345"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HUS29490.1"
                     /translation="MRAIFRRREVEAELDDELQFHLEQSFEANVKAGHSPAEARRLAR
                     IELGGVASVKEGVREARGVRLLFDLGADLRYGLRGLRRNPLFATVAIITLALGIGATT
                     AMFAIVDDVLLHPVPYEQPDRLVRLHASKPAFDKGS"
BASE COUNT         1346 a         2401 c         2314 g         1132 t
ORIGIN      
        1 gccgcaacca cgcgaaggtc acgcgcgatc ccgagacggg tcgctacacg atcagtgatc
       61 tgcagtcgag caacggcgtg cgggtcaacg gtcaggacta cggcaaggtt gagctgcgcc
      121 gcggcgacac cgtcgatctc ggccacgtcc gcctgcgctt cgtcgagccg ggtgaggact
      181 tcgtcttcaa ctcgtcgatg gtcgcggaca tcccggagac cggcagcaag cgcgggctcc
      241 tcgtcgcgat catcctgggc gtgctcgttc tcggtggcgt cggcgcgttc ttcttcttga
      301 acaagaagga caccacgcaa accgccggcg gtcagaagac cgatctcggc agccaaaagg
      361 gctcgggcga cgtcgtcgcc gacaacggca acggcggcgg ttcggagatc gccgtcaacc
      421 ccgtgacgac cgacggcagc ggcagcgaga cgaacacgat ggtgaagccg ccgcccgatg
      481 acgtggccgg caacctcgcg ctcgagtgca agggctacca agccgaggcg aagtggaccg
      541 acctctctcg ctgcggcgag aagctcgcga agctcgacgc gaccgagggc aagaagttcg
      601 gcgcgaccgc tgcgtcggag atcaaggccg agggcacgct ggcggacctg cgcaaagaga
      661 aagacttcgc aaaggccaag cgccagtccg acgcgatctc gaaggactcg tactaccgca
      721 aggacgcgga cgcgcgcatc gaggagctca aggctcacga cgtcggtcag gcggtcagca
      781 aggccgaggc gatgaagtcc aagggccagt gcgaccagat cgcgcggctc gccaacgaca
      841 tcaaagacaa gtacggcccg gaatggggcc agcgcgtcgc cgaacagaag caaggctgcg
      901 tcgagagcac cggtggcaac accggcacga ccaccgtcgt ccagaaggac tgctcgacga
      961 ggctcgtcga caattcgaag agctgcaaga cgcagttctg taaggcgaac ccgaacgact
     1021 ccaattgcgc gaccgggcag acgacggtca ccgcgcccgc gaactgcgac gccgaggccc
     1081 tcaaggacaa gggcatggag aacgtgaaca tgggccagca cgctgctgca ctcgcgcagt
     1141 tcgaagcgtc gctgcgctgc aagaaggacg attacgtcct cgcgctcgcc ttcatggcgg
     1201 catgcaacgc ccagaacgcc gcgaaggcga agtcgtatta cggcaagctc aatccttccg
     1261 cgcagacgaa gtacaaggtg atgtgcattc gcaatcacat cgaccccgcg gtgccgtagc
     1321 accggcccga ccgcatcacg ggtaatcgag atctctcgaa acccttttgg tgccagtagt
     1381 ttgaccacag ggatcggact ggtgtaacca gggctcagcc tgggtgcact agctcggagg
     1441 ctacatgtcc ggaggcctac ctgtgatgag agttcttggt tcggcggcac tcatgctcgc
     1501 gctcgtggga tcgaccgcgc acgcgcaaaa cgcgggcggt gacgtcccgc tcgacacatt
     1561 ccgtcccgcg atggactcgc gcggttacct cacggtgaac gcgtcgcaag ttctcgggca
     1621 caaggagctg tcgttcggcc tcgggtcact cgactggggc tacaagatgc tcaacctcga
     1681 gggcggcggc agtacgtacg atgtgaacaa catcgtcacg gcgacgctga tcgcggcgtt
     1741 cggcttgaag gcgggaccgg ccgagctcga gttcggcgcg agtgttccgt tcgtgatcct
     1801 cttcggcgat cgcggtcccg ataacctcgg cgatccgaac aacgccaacg acgaccagtt
     1861 ctacaagctc gacggtcagg gcctcggcaa catcggcctt cacttcaaga cgcgctttct
     1921 caagacgagc cgcccgccgc acctcggcat cggcgtcatc gccagcatct acctgccgac
     1981 ggcgagcccc gactcgcgct ggctcggcga caagaagccg atcccgcagg tcatcggcat
     2041 catcgacaag gaattcggca gccgcggtca gcttcgcctc gccgcgaacg ccggcattcg
     2101 cattcgcccc gacacgacgt ggacgaacaa cgacccgacc gacggcaaga tgcccgcgcc
     2161 gaacccgccg gttcccacca cgggcgcatc ggttacggcc ggcacggaga tcccgttcgg
     2221 cgtcggcgtc gcgtacgcga tctcggtgca gaagttcgac gtcgtcggtg aagtgatcgg
     2281 cgcggtgccg gtcgccggca acgagaacta ccagccgctc gaagcgatcg gcggcatcaa
     2341 gctctacctc gcgcgcaact cgttcctctc gctcggcggt ggcgcgggcc tgctccgcaa
     2401 gaaggagacc ggcgcgaacc ccgacgtgcg cgcgttcatc ggcatcgtgt tcgagccgaa
     2461 catcggcgac cgcgatggcg acggcatcaa ggacgacatc gacaagtgcc cggacgaccc
     2521 ggaagactac gaccgcttcg aggacgaaga cggctgtccc gagcccgaca acgatcgcga
     2581 cggcatcctc gacgaggacg acaagtgccc gaacatcccg gaagacaaag acggcttcca
     2641 ggatgaagac ggctgccccg aaggcaacaa gaacgatcgc gacggcgacg gcatcctcga
     2701 cgatgtcgac aagtgcccgg acgatccgga agacttcgac cagttccagg acgaagacgg
     2761 ctgccccgat cccgacaacg atcaggacgg catcctcgac gtcgacgatc tctgcccgaa
     2821 cgatcccgaa gacaaggacg gcttcgagga cgaagacggc tgccccgatc tcgacaacga
     2881 caaggaccgc atcctcgaca aagacgacaa gtgcccgaac gagcccgaga cgtacaacgg
     2941 cttcgaggat gaagacggct gtccggatcg cggacgcgtc gtggtgaccg acacgtcgat
     3001 cgagatcctc gacatggtgt acttcgagta caacaaggcg atcatcaagt ccgagtcgtt
     3061 cccgatcctg gacgcggtcg ccgctacgct gcagggcaac cccagcatct cgctcgtcga
     3121 gattcagggt cacaccgacg agcgcggcga tgacgcctac aacctcgacc tgtcggaccg
     3181 ccgcgcggcg tcggtgaaga agtacctcat cgacaagggc gtcgaagaga agcgcctcga
     3241 gtcgcagggc tacggcgaga cccagccgct gattcgcgag cacaacgaga aggcgtgggc
     3301 gaagaaccgc cgcgtcgcct tcctcatctt gaagcgcgcg accgactaac cccgccgctt
     3361 ctggctctcg agaaaggctc cactcttggg gcctttcgca ctttcgggca cgcggcgtgc
     3421 cggcaagcta tgctccgcgc atgatccgtg gcctcctctg gctcgtcgcg attgtcgcgt
     3481 tcatctggtt tggcgccacg gtgaagctcg gccacaggac gttcttcgga cacgtccgcg
     3541 cgatctggca cacggacgag gcgcaggact tgaagcacgg cgtgcaggag aaggcagggc
     3601 cggcggtgca caagctcgag aagggcgtca aagccggcat caacgcgatg aaggacgaag
     3661 gctccaacgg ctcgggcgca ggctcggcgg cgccggttca cccgtagtca tgaagttccc
     3721 gctgacccag cagctcatcg acgaggcaaa gcgcttcgag ctgcgctcgg catgtcgcga
     3781 ctgcttctac ttcgacgctg ccaaagacgt ctgccgccac gactggcccg acgagggtca
     3841 gcgacgttgg ccgctggatg cgaaagactc caacggaaat ctcccgacgg acgtcgcgtt
     3901 ctgcaaagaa ttcgagctta agtagccgcc gccgctcaac tgcgaacact gccgacgggg
     3961 tgtgtgttta ctatcgggat cgtgctcgat ctcgttctct ctcctgaggt ggcggaagcg
     4021 cgtgtcgacc ggcgagcggt cgtcgcgctg gaatcaacgc tcgtcgctca cgggttgccg
     4081 tatccgcaga atctcgaagt cgcccaagag ctgcagcagg cagtgcgcga tgcgggtgca
     4141 atccccgcga cgatcgcgat cgtcgacggt cgcccgcaca tcggcctcac cgatgcgctg
     4201 ctcgagcggc tcgcgaaaga cggcaagacg ttcgcgaaag cgggctcgac ggatctcgcc
     4261 gtgcacatga cacggggcaa gtccgcggcg accacggtca gcgcgaccgc gatgctcgct
     4321 gcacgcgcga agatcaaagt gttcgcgacg ggcggcatcg gtggcgtgca tcgcggcgat
     4381 gcaggcgatg tctcgcacga cctcgtcgca ctcgcgaaga cgccgatcgc ggttatctcc
     4441 gccggcgcga aggcgatcct cgatctccca cgcacgctcg aaacgctcga gacgctcggc
     4501 gtgctcgttg tcggttaccg caccgatgag atgcccgcgt tctacaccgt gcgctcgggc
     4561 ctgcgcctcg agcacttcgc gggcaccgcc gcggatcttt cggcgatcct caagatgcac
     4621 tgggaggagc tcggcggcgg tggcgtgctc gtcgcaaatc ccatcccgtc ggaggcatcg
     4681 ctcgacgagc gcctcatctc cgacgcgatc gcgacgtcgc tgctcgaggc ctcggccgcg
     4741 ggcatcacgg gcaaggcgct gacgccattc ctgctcgcgc gcctcgccga cgtcacgggc
     4801 ggcgcatcgg tccgcgcgaa ccgcgcgctc gcgctgcata acgcgaaggt cggcgcggag
     4861 ctcgcgatcg cgctcaatcc gcatccgatc gaaggcgcac tcacgcagcg cgctctgctc
     4921 agccgcatgt gtaactagcg cgcgcggcgc cgctcgttag ctcgcgagcg agtagacgat
     4981 cagcgctttg ccgccgcggt cgaatccggt cgagcgctcg agcagccaga agaaatcgcc
     5041 gacgacgccg aacctgtggc gcacgccctt cgcgaggatg cgcgccttgc gcgtcgcttt
     5101 gccgtcggcg ccgagcttga agatgtcgag gtactcgggg tccgccttct tgcgcgcaac
     5161 cgcgtccgcg ttcaccggat cgaccttcag cacgatccag gccgtgccgt cggcgttgac
     5221 gagaccctgc atcgacttcg ggtcatacga cgcgagcggc tgatcgaggt cgaccgtctt
     5281 catcgcgccg ccgcgccaca cctggatgct ctgattgtcg ggcgtcatgc gcagaaactc
     5341 ggcctggcca ttcgcatcgg cgagcacctg gaagcgcttg cgctgctcga acagatcgcc
     5401 aatcggtttg ttctcgatgt acttgccggc aacgacgtcg tagaccgctt cggtatcggg
     5461 cgagcgctgg ttctccttct tcacccactc gccgcccttc gtgccgatcg cgcgcgtcca
     5521 gccgtcggcc caatggttga cggtgaggtc gatgcgcttg tcgtgcgcgc cctcgagctc
     5581 cagcggcttg ctcgtgatga gccgcttgcc gctctccagc acaaacacct cgacgtgatg
     5641 cttcacgccc ttcttcgtcg gatcggcgaa gtgcgccacc acgcgctggg cgctgtcacg
     5701 cttcatcacg ttgatgtgcg ccgcgggccc gaccttgtac acgggcgcgg ccgggctctt
     5761 cgccagctcg acgagcgcgc cgacctgctt gccctcctcg gtcgcgccga cgacaaagat
     5821 gcggccctgc ccgacaaacc gcagcgagat cgggcgcagc gtcacgggcg acagatcgac
     5881 gacgatctct ttcttctcct ccgcgcactt cgcgcagccc agctgcacga catgcagctc
     5941 cgccttcgtc gatgtatcgg ccacgatgta cgcgatgcgc tgattctcgt atgcgacgac
     6001 atcgtcgacg aatcccgcag gcggcgtgag cctcatgatc tcgttggcgc caacggtctg
     6061 cggcagcgca gcacccttcg tgaccggcgt tggcggcgtc gtcgtcggcg ccgtcggcgg
     6121 agtcggcgct gctttgtccg catgagccgc catacccgcc aacgccacca tcacaacgga
     6181 ggacagaaac cgattccgag cacccataac gcagtgtcgt gccacggccg ccaccgtcgg
     6241 tgcaagccga atcccggcag ggcgctcacg ccgtgacgaa cgcttcccct tgcgcttcta
     6301 caggagtcgc gctacctctt ctgtagaagc aacaggggag cgacgtgtcc gacgacaaga
     6361 tcgatctgcc gcaaggaacc ctcgagctcc tcgtcctgaa gacgctgggc ctcggcgcgc
     6421 agcacggctg ggcgatcagc gaacgcatca agcagatgtc gcgcgacgcg ctgcaagtcc
     6481 agcagggctc gctctacccg gcgctgcacc ggatggagcg ccaaggctgg atctccgcga
     6541 agtggcgggc gtccgagaac aaccgcaaag cgaagtacta cgagctgacg gccgccgggc
     6601 gccgccgcct cgacgcgacg acgcaagaat ggcgccgcct catcgccgcg gttcagctcg
     6661 tcctcgacgg aggctcgtga cgatgcacgc gccgcaagcg acggtcctcc gccgcctgac
     6721 gacgagcttg tcgcgttcgt gtcggaggac gcgcgatgtt cgctgatctg cgttaccggg
     6781 tgcgggcgat ctttcggcgg cgcgaggtcg aggcggagct cgacgacgag ctgcagtttc
     6841 acctcgagca gtcgttcgag gcgaacgtga aggcggggca ttctccggcc gaggcgcggc
     6901 ggctcgcgcg gatcgagctc ggcggcgtgg cgtcggtgaa agagggcgtg cgcgaggcga
     6961 ggggcgtccg gctgctgttc gatctgggcg cggatctgcg atacgggttg cgcgggttgc
     7021 gacgtaatcc gctgttcgcg accgtcgcga tcatcacgct ggcgttgggc atcggcgcga
     7081 cgacggcgat gttcgcgatc gtcgacgacg tcttgctgca tcccgtgccg tacgagcaac
     7141 ccgatcggct cgtgcgcctg catgcgagca agccagcctt cgacaagggt tcg
//
LOCUS       DATLBZ010000252         3881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_271253, whole genome shotgun sequence.
ACCESSION   DATLBZ010000252 DATLBZ010000000
VERSION     DATLBZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271253"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1223)
                     /locus_tag="VMZ53_13350"
     CDS             complement(63..1223)
                     /locus_tag="VMZ53_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUS29491.1"
                     /translation="MSRIALALLVAGVTAGCARVPLHQADAMSAVPREPLGEARLSFK
                     WKFETADRRTEVTPQEFASPAVWADTLYVGSARGWFYALRATDGRVRWRKLLGSVACA
                     PLVDRGALYVGTNDGFLVALDSQTGVEKWRYQSRGPISQTPAATRDLIVFSNEADQVI
                     AVDAITGKFKWQYKGETPEEYTLRGHAGVSIDGDLIYTGFSNGSLVALRRDTGTLAWS
                     TSLKGDADRFTDVDATPLIMEGMVYASSSSGGVYAIEKATGLVRWRVPFWDASQPSST
                     GNVGGIATDGKAIYVSVADLGTYAIDLGGNVLWRVGAHGGGEPAEPVVHGDLIVYSLA
                     NAGLFVAERKTGNTLEYFDPGDGISAQPTVTGDGRLFVISNLGILYAFDLED"
     gene            complement(1220..1999)
                     /locus_tag="VMZ53_13355"
     CDS             complement(1220..1999)
                     /locus_tag="VMZ53_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29492.1"
                     /translation="MAAKDDKKETKDSKKDPAKPGFDPKPVQLGGESLIERLLPYMKQ
                     IVYVLVGIAIIISIVFMVRHFKERGREKTTNKLVKVLEIADREVKDPNEPDPMADPNK
                     KKEPTFASSKERAEAVLAALSKEGATATTAYKASLLMQAGKLDEAIAEYRKGEDGKGL
                     DGVLSREGLGIALETKALAEKDATARQKGLEEALAVFQKMQPDEKGPRIAYAYYHQGR
                     LLAHLQRAAEARTALEKAKELGEGSELPTLIEERLAMLGGS"
     gene            2033..2596
                     /locus_tag="VMZ53_13360"
     CDS             2033..2596
                     /locus_tag="VMZ53_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29493.1"
                     /translation="MALTARPLILLPALAIFATPTRAHAEGERAFSVGLGWATFSTPG
                     VKMGNMEPPQVSPDVGGALALTYEHAIGSDFSLRGEAAFGVFYGGQAKDQTATSYAGL
                     GDIGATFRFDVLKYVPYAFGGIGAVQTTGGPILAGTNFVIAVGGGVDMLVSRKRSYGI
                     EGRLASFGGDVTIFTFGLRGTVRWGYF"
     gene            2620..2940
                     /locus_tag="VMZ53_13365"
     CDS             2620..2940
                     /locus_tag="VMZ53_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUS29494.1"
                     /translation="MTTVASADPPAPTPKLLELGKQVYTVNCAACHGEKGDGKGPVAF
                     SINPPPRNFQKDPFKQGDTVPQIFTTLTNGLAKTKMVAYKQLPEEQRWALAYFVRAFR
                     IKGS"
     gene            complement(2946..>3881)
                     /gene="der"
                     /locus_tag="VMZ53_13370"
     CDS             complement(2946..>3881)
                     /gene="der"
                     /locus_tag="VMZ53_13370"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HUS29495.1"
                     /translation="PKKEPVDEPDPLFDADPEDEFDDEEDEGEGEGGRRKERADNIPA
                     GPLRVAFVGRPNAGKSSLTNRLLGEERSLVHDEAGTTTDPVDAQFSIGGRDYILVDTA
                     GIRRKAKIDVEIEKISVSMALGQIERADVAVLVIDGGLGPAEQDARIAGKIEEAGRAL
                     VIALNKSDLVSGEAKKSTLRDVTKDTFHFLEWAPTVMLSAQRGDGVDRLLDLVDQVAG
                     EHRRRIPTAQLNRFFADVIEEMPPPLHHGRAIRINYITQAAIRPPTFILWANSLEGLA
                     PSYKRFIANRLRQAYGFRGTPIRIFVKARKDRHKD"
BASE COUNT          618 a         1266 c         1270 g          727 t
ORIGIN      
        1 tcgctcggcc actgcaagta agcgcttatc gatgccgcct gcgcggcgcc gcgagcgagc
       61 gctcagtctt cgaggtcgaa cgcgtacagg atgccgaggt tcgagatcac gaacagccgg
      121 ccatcacccg tgaccgtcgg ctgcgccgag atgccgtcgc ccggatcgaa gtactcgagc
      181 gtgttgcccg tcttgcgctc ggcgacgaac aggcccgcgt tcgcgagcga gtagacgatc
      241 aaatcgccgt gcacgaccgg ctccgccggc tcgccaccgc cgtgtgcgcc gacgcgccac
      301 agcacgttgc caccgagatc gatcgcgtac gtgccgaggt cggcgacgga gacgtagatc
      361 gccttgccgt cggtcgcgat gccgccgacg ttgcccgtcg agctcggctg cgatgcatcc
      421 cagaacggca cgcgccagcg cacgaggccg gtcgccttct cgatcgcgta gacgccgccg
      481 gacgacgacg acgcgtagac catgccctcc atgatgagcg gcgtcgcgtc gacatcggtg
      541 aagcgatcgg catcgccttt gagcgaggtc gaccacgcga gcgtgccggt atcgcggcga
      601 agcgcgacga gcgagccatt cgagaagccc gtgtagatca ggtctccatc gatcgagacg
      661 cctgcgtggc cgcggagcgt gtactcctcg ggcgtctcgc ctttgtattg ccacttgaac
      721 ttgccggtga tcgcgtcgac cgcgatgact tgatcggcct cgttcgagaa cacgatgaga
      781 tcgcgcgtgg cagcgggcgt ctggctgatc ggcccgcggc tctggtagcg ccacttctcg
      841 acgcccgtct gcgaatcgag cgcgacgaga aacccgtcgt tcgtgccgac gtagagcgcg
      901 ccgcgatcga cgagcggtgc acatgcgacc gagccaagca gcttgcgcca ccgcacgcgg
      961 ccatcggttg cgcgcagcgc gtagaaccaa ccgcgcgccg agccgacgta gagggtgtcg
     1021 gcccagacgg ccggagatgc gaattcctgc ggcgtgactt cggtgcggcg atccgccgtc
     1081 tcgaacttcc acttgaacga cagacgcgct tcgccgagag gctcacgcgg gaccgcgctc
     1141 atcgcgtcgg cttggtgcag cggtacgcgc gcgcaaccgg cagtgacgcc ggcgacgagc
     1201 agcgcgagcg cgatgcggct cacgagcctc cgagcatggc gaggcgctct tcgatcagcg
     1261 tcggtagctc actgccttcg ccgagctcct tcgccttctc gagcgcagtg cgcgcttcgg
     1321 ctgcgcgttg caggtgcgcg agcagacggc cctggtgata gtacgcatac gcgatgcgcg
     1381 ggcccttctc gtcgggctgc atcttctgga agaccgcaag cgcttcctcg aggcccttct
     1441 gacgagcggt cgcatctttc tctgcgagcg ctttcgtctc gagcgcgatg ccgagacctt
     1501 cgcgcgagag cacgccgtcg aggcctttgc cgtcttcgcc cttgcgatat tcggcgattg
     1561 cttcgtcgag cttgccggcc tgcatcagca gcgaggcctt gtacgcggtg gtcgcggtgg
     1621 cgccctcttt cgagagagct gcgagcacgg cttccgcgcg ttccttggag ctcgcgaacg
     1681 tcggctcctt cttcttgttc ggatccgcca tcgggtcggg ctcgttggga tctttgacct
     1741 cgcggtcagc gatctcgagg accttcacga gcttgttcgt cgtcttctcg cggccgcgct
     1801 ctttgaagtg ccggaccatg aagacaatcg agatgatgat cgcgatgccg accagcacgt
     1861 acacgatttg cttcatgtac gggagcaggc gctcgatgag cgactccccg cctagctgga
     1921 ccggcttcgg atcaaagccc ggtttcgcag gatccttctt ggaatccttg gtttcctttt
     1981 tgtcgtcctt ggcggccacg gggctcggga tgtagcacaa ttcaggggct acgtggctct
     2041 gaccgcgcga cccctcatcc tgttgcctgc gctcgcgatc ttcgcgacgc cgacgcgggc
     2101 tcatgccgaa ggcgagcggg cattttcggt cggtctcggc tgggcgacgt tctcgacccc
     2161 cggcgtgaag atggggaaca tggagccgcc gcaggtgtcg cccgacgtcg gtggcgcgct
     2221 ggccctgacg tacgagcacg ccatcgggag cgacttctcg ctgcgtggcg aagcggcgtt
     2281 cggcgtgttc tacggcggcc aagcgaaaga tcagaccgcc acctcgtacg caggcctcgg
     2341 tgatatcggc gcgacgttcc gcttcgatgt cctgaagtac gtgccttacg cgttcggcgg
     2401 tatcggtgcg gtgcagacga cgggtggccc gatcctggcc ggcacgaatt tcgtgatcgc
     2461 ggtcggcggc ggcgtcgaca tgctcgtcag tcgcaagcgc tcgtacggca tcgagggccg
     2521 gctcgcgtcg ttcggcggcg atgtcacgat cttcacgttc gggctgcgag gcaccgtacg
     2581 ctggggctac ttctgaaggc cgcgctgctt ctgtgcgcgc tgacgacggt cgcatctgcg
     2641 gatccgccgg cgcctacgcc gaagctcctc gagctcggca agcaggtcta caccgtgaac
     2701 tgcgccgcgt gtcacggcga aaaaggtgac ggcaaaggtc cggtcgcgtt ctcgatcaat
     2761 ccgccgccgc gcaatttcca gaaggatccg ttcaagcagg gcgacaccgt gccgcagatc
     2821 ttcacgacgc tgacgaacgg cctcgcgaag acgaagatgg tcgcctacaa gcagctgccc
     2881 gaagagcagc gctgggccct cgcctacttc gtgcgcgcat tccggatcaa gggatcataa
     2941 aggtctcaat ccttatggcg gtctttacgc gccttcacaa agatgcgaat cggcgtgccg
     3001 cggaatccgt acgcttggcg taggcgattc gcgatgaagc gcttgtagct cggcgcgagt
     3061 ccctcgagcg agttcgccca caggatgaat gtcggcggac gaatcgcagc ctgcgtgatg
     3121 tagttgatgc gaatcgcgcg accgtgatga agcggcggcg gcatctcttc gatcacgtcg
     3181 gcgaagaagc ggttgagctg cgcggtcgga atccgacgac gatgctcgcc tgcaacctga
     3241 tcgacgagat cgagcaaccg atcgacgcca tcgccgcgct gcgcggagag catcacggtc
     3301 ggcgcccact cgaggaagtg gaacgtgtcc ttcgtgacgt cgcgcagcgt cgactttttc
     3361 gcttcaccgc tcacgagatc cgacttgttc agcgcgatca cgagcgcacg gcccgcttct
     3421 tcgatctttc cggcgatgcg cgcgtcctgc tcggcggggc caaggccacc gtcgatgacg
     3481 agcactgcga cgtcggcacg ttcgatctgg ccgagcgcca tcgacacgct gatcttttcg
     3541 atctcgacgt cgatcttcgc tttgcggcga atgccggcgg tgtcgacgag aatgtagtcg
     3601 cgaccgccga tcgagaactg cgcgtcgacc ggatccgtcg tcgtcccggc ctcatcgtgg
     3661 acgagcgagc gctcttcgcc gagcaagcga ttggtgagcg acgacttgcc ggcattcggg
     3721 cgaccgacaa acgcgacgcg cagcgggcct gccggaatgt tgtcggcgcg ctctttgcgt
     3781 cggccgccct cgccctcgcc ctcgtcttct tcgtcgtcga attcgtcctc gggatccgca
     3841 tcgaacagcg ggtcgggctc gtcgacgggc tccttcttcg g
//
LOCUS       DATLBZ010000253        16064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9209311, whole genome shotgun sequence.
ACCESSION   DATLBZ010000253 DATLBZ010000000
VERSION     DATLBZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16064
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9209311"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1552
                     /locus_tag="VMZ53_13375"
     CDS             71..1552
                     /locus_tag="VMZ53_13375"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HUS29496.1"
                     /translation="MRGLVVMLVVGACAHTPNLALRPDPSPPAGSTTETIATPDGTQL
                     LARHWAATGDTRGVLVIMHGLKDYSARYADLASQLAATGYSVYAFDLRGHGRSSGPRV
                     APAHWTDYVADLDRFLSLVEKREPGRPIFLFGHSMGGAIAARAAEIHTPQLAGLVLSG
                     PALAIDAPPLLIAATRMSGFLTPKFPALKLPNGDFSSVPGSGDAMSRDELVSNPPAPA
                     RTAAGLVDGMRAIWGDADRLTMPVLALHGTADKLTAPSGSRALIARIPSTDKALKIYA
                     GFFHDLLHEPKRADVEADIHAWLDAHTGGASFTQPPPYVEHLAGDPRGWTQAIEFAGG
                     ISRGFDEKVTRFGGEFALSLARPRPLGWHGQLTARYANTRYALALKPIGVAVRFGGAV
                     VGLSGGGALLTGAHPAGAASAWYEQPAGPVHLGIRADYEHAFFDGARNVGLLIGSLRL
                     GGDRHYWPHAVAGVGPIVSGGYECQGDTCGFVALAGLQLYGAD"
     gene            complement(1537..3939)
                     /locus_tag="VMZ53_13380"
     CDS             complement(1537..3939)
                     /locus_tag="VMZ53_13380"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HUS29497.1"
                     /translation="MSDDARRFADELLEKLYVELAKFPGVASKNDHYLALSYAVRDRL
                     LHRWVDSARAVLLLKQRTVVYLSAEYLIGPQLGSNLQALGIELEVKQAFSSASGYQLD
                     DLLEHEEEPGLGNGGLGRLAACYMDSLATLDIPAVGHGLRYEFGIFDQDIRDGWQVER
                     TDRWLRHGFPWEIRRYDIEHSVGFGGYTEFSPEGVRWIPERVVKGVPFDVPVAGYGTP
                     TTNFLRLWSAFAAEEFDLDAFQVGEYWRAVDSKIRSENLTKVLYPNDSSPAGKQLRLE
                     QQYFFVSCALQDCLRLLFMSGATVSDIPRLLAIQLNDTHPTLAIPELMRLLMDVHHVS
                     WNDAWDITRQTFAYTNHTLLPEALETWPLPLFRRLLPRHLEIIYEINRRFLDEVRAKF
                     PGENERLSRMSLIGEQGEKQVRMAHLATVASHHINGVAALHSRLLRETVLKDFAEMWP
                     ERFTNVTNGVTPRRFVSLANPRLSHLLDATIGPGWLRDLDKLRELEKLADDASFRDRW
                     RAVKHANKAALAAAYGNGFDPASLVDAQCKRIHEYKRQHLNLLHAIVLWQRLRSGSTR
                     DVPRTIVLAGKAAPAYRAAKLIIRLAHGIAEAIESDPNAHSQLRLVFIPDFSVKNAQK
                     IYPAAELSEQISTAGMEASGTGNMKFTLNGALTIGTLDGANVEIREHVGADNFFLFGL
                     TAEEVLARKRAGYDPRTELARDGELQQAIDLIASGEFSRGDRELFAPLVRDLVERDPF
                     LVLADFRAYVEAQEQVAKAYRDQTWWQRASILNVARAGYFSSDRSIREYAEQIWRVQS
                     AP"
     gene            complement(3962..4264)
                     /locus_tag="VMZ53_13385"
                     /pseudo
     CDS             complement(3962..4264)
                     /locus_tag="VMZ53_13385"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            complement(4319..6349)
                     /locus_tag="VMZ53_13390"
     CDS             complement(4319..6349)
                     /locus_tag="VMZ53_13390"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HUS29498.1"
                     /translation="MSQEKNRVLAGELVSRASVEAATGSLDDALNACDFDLAASIALS
                     RAKAGDAPDAHAISRILAGLEFPPIATALVATVRDRTALLDHVAAGLYPRTKDAGEIE
                     TIVLYAAWKAGASVEKIIPPLRRLAARQLTAEAYALLATIAEKIDDANVRAATKPIAT
                     FAKEYAKSVAADDKALNQPIDAAIAALPAAIESSRGGFTVRAAATVGRNDPCPCGSGL
                     KYKKCCADKPQTAAAPSPIPGVSWDEFIAGDKLTPEHIDELALKDLVRVDVSKVSSEC
                     LGHLASRFAAARQWTHVERVVEQASGRDGAIGQNLRELFVDHLLEVEDVEWAKHHLAG
                     MPREDANLFHLELAIRERGATAYNALVDFARTAVENAKNDDGKDNLATVELAHVLLRA
                     EPVLGILAARACIGAWLVDDAELLLEAVEEARDRLNLPPTDPAWDVYDTIVAGDTKRG
                     KKGAKAEGDVAIREAMAESSAKIDQLERALAAVRTELADAKTRPAAELVRQGSSTGSD
                     KLGERVRELEALIREGNEERRELRKQLEAAASTDSAQEPRPGAHDDDRPRRGRHLDDD
                     DAGDDGDALEPGSRNVAIPRFERRALDAIAEVPATVAAETMRTVGTLAAGDPFAWRGV
                     KAAKDMPRQVLMQRVGIHHRLIFRSEGGNLDVLDLITREQLQTTLKRLRQGR"
     gene            complement(6346..7296)
                     /locus_tag="VMZ53_13395"
     CDS             complement(6346..7296)
                     /locus_tag="VMZ53_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29499.1"
                     /translation="MSQKFQASVQHRGDVSYVKLGGVIDEDNELGDLVDKIPSGTAVI
                     DLGEIERINSCGVRDWVNWLSKLESNGTRSVLVECSPAIVAQINLVNNFTGNGVVKSF
                     YVPYFCPECDEEKVLLVEAGDMGPPPHEPPTCRCDECDLVMDFDDMPDSYFAFLANQK
                     KLAEPDKVNGAMKELARGSGADSGEKSGAKGKVKSRSSAPQIGGAPGSKPSVPSLPSI
                     QRTSTQPATGATHSSGPRLPSGKSGSSSKPPSKPPSAPRLGSPSKAPSNSHITPGMSL
                     RMSPSSNAMAAPGKSNALVFTLVGILVAAIAVLAYLVLTK"
     gene            complement(7430..13741)
                     /locus_tag="VMZ53_13400"
     CDS             complement(7430..13741)
                     /locus_tag="VMZ53_13400"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS29500.1"
                     /translation="MGEGPPIRGSSTKPPPPPARAPIAPAPAKTDVVPAQRDPELDVG
                     WAGGEEVSEITKNLEAPDATQLVHKMLELVASEAEALLTAEDPDGRLADLNVRTAFAS
                     WDGLHEPDEALRYLELAESHPLAPRLRLSAALAAHSPEALAQAQGRIESLPASQTTTA
                     LLIEVTEAWLFRYGRADVAAALVERVLEAEIPAPWREHVTNLAALAKAATGRQWDRVV
                     EIRRAAAERSASLHDIAASAALVLDRGRDALGALQLCWGAIERLEGTDADVRDTQPSA
                     AVNRAGQLRLIDVGIDAAMRAGDERVLELLDRRAELVANLPGGALEALMTRHAVAAAL
                     SRDGQHPEASALWAQLADDPVASAPATARRVALLAGAWAAAAAGDAKAALAARRRLVD
                     SECPEAAAAHAWRALELAAAVGESPQVLSDLAHAVVDAADTPVAEWWLDLLDHPAPTQ
                     GTVARLEAREGLALRWAAELAEKLGNIPKALELWRRACALDGRLGTEYDHLVRLLRAM
                     DEDELAEAYSLWALAETDMRCASALQAARGIVDLVRGDFVEAEETLQRAADLDPKDSF
                     CRAALAAVYRAGKRYDMLAQVLAELSTSLTSRDARASAAREYAELLDEHLGDPMAARQ
                     ALERMIAERPDDDDAMLTLAKLYDRDQQWARSIELRRKAVELSTTPDERAVRWIDIAV
                     REERRGDRDAAIAALDKAAQTGVRKIEVMREQARVHRQAGRFDKALEIVRSELAGEPG
                     LARRMQLRTEEAQLLTALDSEPEAVVAAYLDVLSIEPDQTEALAGIEAPARKLELWDE
                     LARAFRGAPQTPRNLEVLAEALAKIAEWSELAEVRRKQLEAAGSTDEKARRAAELARL
                     YEHELGDNDAAIRMLVVAQASLPDAQRQKDLLRLLRAAERWGELAQALERELPQIRPS
                     DVLRQADVLLELGALRADKLNRLPEAAQAFESVLERDPKNSTAAERLEKLYEQLGRDR
                     ELARMLELRAEATQDLVARAALMARVATLRTNRGDVDGAIAAYISAFSADPTNRDVFT
                     AMERVCYKAERWAAAMQMYEIAIQHVETGQGRAYRLGDLYSRRGNVQLSFLGQVDAAI
                     ASYQKVVEVDSQPQAAVKVLEDLSKQRGDWKPLIEAYEKRAETQRDPQRKADALRAAA
                     ALATDRMDDVRNSVRLNRKLLTVDPNDAGAVSTLEKYFEETQDKSGLIDILKTRLAHS
                     QTHGGAESVEIMKRIARVSEEGARDVDTATEHYQKVLEVQPENRDALDALGRIYESTE
                     QWADFIDVTRRLIKITNDRNTKALLYFKCGSVMEAKFGREHDAIRYYDAAIKTSPNCM
                     PAVHGLRDLYRRREEWPRVIETLELEVKLWSDDKERAGVFAQIGRIYAQQLNDPDRAM
                     QYYDSALAVDPDCLPANQALFEHYFERGEWDKAMPIATSLAQKAMRDGDPGTRSEFYR
                     KRGVVARMAGDLKSAADSFVVALEIKPLNTAALDDLGSLARDQPDAWDFDATYKELEK
                     VYKKRDDAGPLFARVNVARAAIVERDGDLDAAGELYHAALELAPTDLTVLNALVDFYA
                     DMRRWKQAVDAIQKFVEAGASGTERLLALMRQANIHADGEMDPTRAIAVLKHVIQIEP
                     THQDAYYLLAQQCFLVGRYQEARTFIERVIDLATAPGMPLSAEALARYYYYKGRILDA
                     AGDARAAAPQYRRAIEYDPGYAPPALVLARRAADGGDQRQAETLLIEAAHAAMAQGGP
                     RAAVPLQRGLARILLSSGDRPAAIEAYRGILNVEPDGASDRVALAEIYAVDDPTRAIG
                     ELRKVLERDIHHAPAYRLLASFYTRTGDNERATRVLTALDLLGFAEETDRQTVQRLRQ
                     SRQSQPLRRTLDENNRERLMANTAPREPLGEVWTAFAEEITALVAQPSLGENVLPAEQ
                     VDARLLQLAGEIGQLFEVEPEIFVGDKVPGLLAVTAFPRQIVVVDRSLLEEKDLPLRF
                     MFGYAFEAIRGGYAALLQLGARQRRELGQLMRTLISDVEPTGAAGELVRTATVRAAKV
                     LERHAGTRDLDPGSWIDNMLACAKRAGLVACDDFGAAIWMISRLTGELLESNDDTVAL
                     GSVLGGPDLVRFYLSDNYQMIRDLLALPA"
     gene            13840..14730
                     /locus_tag="VMZ53_13405"
     CDS             13840..14730
                     /locus_tag="VMZ53_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29501.1"
                     /translation="MRPRAICICRDDGVCRVVGHALDSAGVEVEHLAQVPTDPGEVAL
                     FVVDRETGATSENDLRGVGAPVVLVGNDLEDEGMINLMHEAPFSHLVRDPADRELGIT
                     SEKLVSGDLFGLEKYLAKGARVGERVVSTDVEKRAAMHEVIAWAEAIGARRPIVHRLA
                     TVIDELLMNALHDAPRESKPVISIEDELAKKAVLRWGADEQSIAVSVSDPFGALRQRD
                     VIDHVRRARDERGRPIAGTTEDPGAGLGLYLVLANVASLIVNVDPGHQTEVVCLFDRA
                     RPGTRAVTSGVRSLHVFQQH"
     gene            complement(14742..15179)
                     /locus_tag="VMZ53_13410"
     CDS             complement(14742..15179)
                     /locus_tag="VMZ53_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUS29502.1"
                     /translation="MKPIRDALELLTAQHEELDELCDRVARSKDAAAFALLADKLPHH
                     LAVEQELLYPAIGAHVSRDVMNEVLAEHVAIKHLLAKLVWAGVEDPEFSGHFECLREL
                     LVGHSAWQEDQLFTTAAESLPADRLAILCDELQAFDNVIAVAA"
     gene            complement(15215..>16064)
                     /locus_tag="VMZ53_13415"
     CDS             complement(15215..>16064)
                     /locus_tag="VMZ53_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29503.1"
                     /translation="PIAGPPPGALSPATFFLRHLALSASLGIVAAFFLPAVATWAATL
                     VRRSVLEIATTIAGAPTRQATPVNAGGSAVILGALPGFASTVIIVITLLTAAWLENAA
                     PVLPPLATLVTLVALSLLSALAIRSSASRVMGDVLRDVSALDRQQLATLEIRPPTALE
                     RTVGKLLGHDGGIVYRKDARLMRRRYPMAFALGFVSFATLVIIGLARPDDPAPWLVVV
                     LAGTGLYAIALARRLFTPPIEMVRLAATLPIPIEARTRAKVMWVFAWLVVFVVVPLGF
                     AAARLL"
BASE COUNT         2503 a         5139 c         5651 g         2771 t
ORIGIN      
        1 gccgcaacca accagcaacg cgaacgctcc gcagcgaatc caccgcatga agccaggcta
       61 tcatggcggc gtgcgtggcc tcgtcgtgat gctcgtggtg ggtgcgtgcg cgcacactcc
      121 aaatcttgca ctgcgtcccg atccgtcgcc gcccgcgggc tcgacgacgg agacgatcgc
      181 tacgcccgac ggcacgcagc tgctcgcgcg tcattgggcg gcgaccggcg acacgcgcgg
      241 cgtgctcgtc atcatgcacg ggctcaagga ctactcggcg cgctacgccg acctcgcgtc
      301 tcagctcgcc gcgaccgggt actccgtcta tgcgttcgac ctgcgtggcc acggtcgctc
      361 gtccgggccg cgcgtcgcgc ccgcgcactg gaccgattac gtcgcggatc tcgatcggtt
      421 cctctcgctc gtcgagaagc gcgagcccgg caggccgatc tttctgttcg gccactcgat
      481 gggtggcgcg atcgccgcac gtgcagccga gatccatacg ccgcagctcg cgggcctcgt
      541 gctctcgggc cctgcgctcg cgatcgatgc accgccgttg ctgatcgccg cgacccggat
      601 gtccggtttc ctgacgccca agttcccggc gctgaagctc ccgaacggcg atttctcgtc
      661 ggtgcccggt tcgggcgacg cgatgtcgcg cgacgagctc gtgtcgaatc caccggcgcc
      721 ggcgcgtacg gcagcaggtc tcgtcgatgg catgcgcgcg atctggggcg atgccgatcg
      781 gctgacgatg ccggtgctcg cgctgcacgg cacggcggat aagctcacgg cgccatcggg
      841 aagccgcgcg ctgatcgctc gtattccgtc gacggataag gcgctgaaga tctacgcggg
      901 cttcttccac gatctgctgc acgagccgaa acgcgccgac gtcgaggcgg acatccatgc
      961 gtggctcgat gctcacaccg gcggtgcctc attcacacag cctccgccgt acgtcgagca
     1021 cctcgcaggc gatccgcgcg gctggacgca agcgatcgaa ttcgcgggtg gcatctcgcg
     1081 cggcttcgac gagaaagtga cgcgcttcgg tggtgaattc gcgctctcgc tcgcgcgtcc
     1141 tcgcccgctc ggctggcacg gtcagcttac cgcgcgctac gcgaacaccc gctacgcact
     1201 cgcgctcaaa ccgatcggcg tcgcggttcg cttcggcggc gcggtcgtcg gcctttccgg
     1261 tggcggcgcg ctgctcaccg gcgcacaccc ggctggtgcg gcgagtgctt ggtacgaaca
     1321 gccggcgggc ccggtgcacc tcggcattcg cgcggactac gaacacgcgt tcttcgacgg
     1381 cgcgcgcaac gtcggccttc tcatcggctc gcttcgcctc ggcggcgatc ggcattactg
     1441 gccacacgcc gtcgccggtg tcggaccgat cgtcagcggc ggctacgaat gccagggcga
     1501 tacgtgcggg ttcgtggcgc ttgccggcct gcagctctac ggcgcggact gaacgcgcca
     1561 gatctgctcg gcgtattcgc gaatcgagcg gtccgacgag aagtagccgg cgcgagcgac
     1621 gttgaggatc gacgcgcgct gccaccacgt ttgatcgcgg taggccttcg cgacctgctc
     1681 ttgcgcctcg acgtacgcgc ggaaatcagc gagcacgagg aacggatcac gctcgacgag
     1741 gtcgcgcacg agcggcgcga acagctcgcg atcgccgcgc gagaattcgc cggaggcgat
     1801 gaggtcgatc gcctgttgca gctcgccatc gcgcgcgagc tcggtgcgcg gatcgtagcc
     1861 ggcgcgtttg cgcgcgagca cctcttcggc ggtgaggccg aacaggaaga agttgtcggc
     1921 accgacgtgc tcgcggattt cgacgttcgc gccgtcgagc gtgccgatcg tcagcgcgcc
     1981 gttgagcgtg aacttcatgt tgcccgtgcc cgacgcctcc atgcccgccg tcgagatttg
     2041 ttcggagagc tccgccgccg gatagatctt ctgcgcgttc ttgacgctga agtccgggat
     2101 gaagacgagc cgcagctgcg agtgtgcgtt ggggtcggat tcgatcgctt cggcaatgcc
     2161 gtgcgcgagg cggatgatga gcttggccgc gcgatacgca ggagcggctt tgccggcgag
     2221 cacgatcgtg cgcgggacgt cgcgcgtcga gccggagcgc agacgctgcc aaagcacgat
     2281 cgcgtgcaat agattcaggt gctgacgctt gtactcgtgg atgcgcttgc actgggcgtc
     2341 gacgagcgat gcgggatcga acccgttacc gtatgcagcg gcgagcgcgg ccttgttggc
     2401 gtgcttgacc gcgcgccagc gatcgcgaaa cgacgcgtcg tcggcgagct tctcgagctc
     2461 gcgcagcttg tcgagatcgc gcagccagcc agggccgatg gtcgcgtcga ggagatgaga
     2521 caggcgcgga ttcgcgagcg acacgaaccg gcgcggcgtg acgccattcg tgacgttcgt
     2581 gaagcgctcg ggccacatct cggcgaagtc tttgagcacg gtctcgcgaa ggagccgcga
     2641 gtgaagcgcg gccacgccgt tgatgtggtg ggaggcgacc gttgcgaggt gcgccatgcg
     2701 gacttgcttc tcgccctgct cgccgatgag cgacatccgg ctgagacgct cgttttcgcc
     2761 tgggaacttc gctcgaacct cgtcgaggaa gcgccggttg atctcgtaga tgatctcgag
     2821 gtggcgcggc aggaggcgac ggaacagcgg cagcggccac gtctcgagcg cttcgggcaa
     2881 cagcgtgtgg ttcgtgtacg cgaatgtctg gcgcgtgatg tcccacgcgt cgttccagct
     2941 cacgtggtgc acgtccatga gcaggcgcat cagctcggga atcgcgagcg tcgggtgcgt
     3001 gtcgttcagc tggatcgcga ggaggcgcgg gatatcggag acggtcgcgc cgctcatgaa
     3061 gaggaggcgc aggcaatctt gcaacgcgca cgagacgaag aagtattgct gctcgagcct
     3121 gagctgcttg cccgcgggcg aggagtcgtt cgggtagagg accttcgtca ggttctcgct
     3181 gcggatcttc gagtcgaccg cgcgccagta ctcgcccact tgaaatgcgt cgaggtcgaa
     3241 ctcttcggcg gcgaacgccg accacaggcg caggaagttt gtcgtcggcg tgccatagcc
     3301 cgcgaccggg acatcgaacg gcacgccttt gacgacgcgc tccgggatcc agcgcacgcc
     3361 ttcgggactg aactcggtgt agccgccgaa cccgacggag tgctcgatgt cgtaccggcg
     3421 gatctcccag gggaagccgt ggcggagcca gcgatccgtg cgctcgacct gccagccgtc
     3481 gcggatgtct tgatcgaaga ttccgaactc gtagcgaagg ccgtggccaa ccgccgggat
     3541 gtcgagtgtc gcgagcgaat ccatgtagca ggcagcgagg cgcccgaggc cgccgttgcc
     3601 gagccctggt tcttcttcgt gctcgagcag atcgtcgagc tgatagccgc tcgcgctgct
     3661 gaacgcttgt ttgacttcca gctcgatgcc gagggcttgc aggttcgagc cgagctgcgg
     3721 cccgatcaga tattcggcgg acaaatagac gacggtgcgc tgcttgagga gcaggaccgc
     3781 gcgagccgag tcgacccagc gatggagcag gcggtcgcgg acggcgtacg aaagcgcgag
     3841 gtagtgatcg ttcttcgacg cgacgcccgg aaacttcgcg agctcgacgt acagcttctc
     3901 gagaagctcg tcggcaaacc gccgtgcgtc gtcgctcatc cgcgtattta accgcagaag
     3961 gctacttcgc gagctttgcg taggccgctt tgtcgaatcc gacgatgatc ttcttgcctg
     4021 cgaccacgat cggtcgcttc aggaggttca cgtccttgac gacggccttc gcaaacgtcg
     4081 cggcgtcagg cggcgcggca acccagccct tctccttgac gtccttgttg cgggtgttca
     4141 gcacggcggc cacggagccc gcgcgttcga tgaggtcctt cacggtcgcc tcgtcgagcg
     4201 gcttcttcgc gtaatcgatc tcggtcatct cgtgtcctgc gctgcgcaag gaactcctcg
     4261 cgtcacgaca ggacgttcaa gtgctcttcc agaacagggt gcccttcatg ggcacagctt
     4321 accgcccttg gcgcagccgt ttcagcgtcg tctgcagctg ttcgcgcgtg atcaggtcga
     4381 ggacatcgag gttgccgccc tcggagcgga agatgagccg gtggtggatg cccacgcgct
     4441 gcatgagcac ctgccgtggc atgtccttgg ccgccttgac gccgcgccac gcaaagggat
     4501 cgcccgcagc gagcgtgccg acggtccgca tcgtttcagc ggcgacggtc gccggaacct
     4561 ctgcgatcgc gtcgagtgcg cggcgttcga agcgcgggat ggcgacgttg cgagagcccg
     4621 gctcgagcgc atcgccgtcg tcgccggcgt catcgtcgtc gaggtggcgg cctcgtcgag
     4681 gacgatcgtc gtcgtgggcg cccgggcgcg gctcctgagc gctgtcggtg ctcgcggcgg
     4741 cttcgagctg cttgcggagc tcgcggcgct cttcgttgcc ctcgcggatg agcgcttcga
     4801 gctcgcgcac gcgttcgccg agcttgtcgc tgcccgtcga cgagccttgg cgtacgagct
     4861 cggcagccgg ccgcgtcttc gcatcggcga gctcggtgcg aacggccgcg agcgcgcgct
     4921 ccagttgatc gatcttcgcg gaggactccg ccatcgcttc gcggatcgcc acgtcgcctt
     4981 cggccttggc gcccttcttc ccgcgcttgg tgtcgcccgc aacgatcgtg tcgtagacgt
     5041 cccacgccgg atccgtcggc ggaagattga gccggtcgcg tgcttcctcg acggcctcga
     5101 gcaacagctc cgcgtcgtcg acaagccagg cgccgataca cgcgcgcgcc gcgagaatgc
     5161 cgagcacagg ttccgctctc agcagcacgt gcgctagctc gacggtcgcg aggttgtcct
     5221 tgccatcgtc gtttttggcg ttctcgacgg cggtccgcgc aaaatcgacg agcgcgttgt
     5281 acgcagtcgc gcctcgttcg cggatcgcga gctcgagatg aaacaggttt gcatcctcgc
     5341 gcggcatgcc ggcgaggtga tgctttgccc attcgacatc ctcgacctcg agtaaatgat
     5401 cgacgaagag ctcgcgcagg ttctggccaa tcgcgccgtc gcggccgctg gcttgctcga
     5461 ccacccgctc gacgtgcgtc cactggcgag ccgctgcgaa ccgagacgcg aggtgcccga
     5521 ggcactcgct cgacaccttc gagacgtcga cgcgaacgag atctttcagc gcgagctcgt
     5581 cgatatgctc gggcgtgagc ttgtcgcctg cgatgaactc gtcccacgaa acgccgggga
     5641 tcgggctcgg cgctgccgcg gtctgcggct tgtcggcgca gcacttcttg tatttgagcc
     5701 cgctgccgca cggacacgga tcgttacgcc cgaccgtggc agcggcgcga acggtgaacc
     5761 caccccgcga cgattcgatc gccgcgggca gcgctgcgat tgcggcgtcg atcggctgat
     5821 tgagcgcctt gtcgtcggcc gcgacactct tcgcgtactc tttcgcgaac gtcgcgatcg
     5881 gcttggtcgc ggcgcgaacg ttcgcgtcgt cgatcttctc ggcgatcgtt gcgagcagtg
     5941 cgtaggcctc ggcggtcagc tgccgcgcgg cgagccggcg cagcggcggg atgatcttct
     6001 cgacggacgc gcccgccttc cacgccgcgt acagcacgat cgtttcgatc tcgcccgcat
     6061 ccttcgtgcg cggatagagg cccgccgcga cgtggtcgag cagcgcggtg cgatcgcgga
     6121 ccgtcgcgac gagcgccgtg gcgatcggcg ggaactcgag cccggcgagg atgcggctga
     6181 tcgcgtgagc atcgggggcg tcgccggcct tcgcacgcga gagcgcgatg cttgctgcga
     6241 gatcgaagtc gcacgcgttc agtgcgtcgt cgagcgagcc ggtggccgct tcgaccgagg
     6301 cgcgcgagac gagctctcct gcgagaaccc gattcttctc ctgactcact tggtcaggac
     6361 gagatacgcg agcacggcaa tcgctgcaac caagatcccg accagcgtga acacgagcgc
     6421 gttgctcttg cccggcgcgg ccatcgcgtt cgagctcggc gacatgcgca gggacatgcc
     6481 cggcgtgatg tgcgagttcg acggtgcctt cgacggcgag ccgaggcgag gcgcgctggg
     6541 cggcttgctc ggtggcttgc tcgacgagcc gctcttaccg ctcggcaggc gagggccgct
     6601 cgaatgggtg gcgcccgttg ccggctgcgt gctcgtgcgc tgaatcgacg gcagcgacgg
     6661 aacgctcggc ttgctgcccg gcgcgccacc aatctgcgga gcgctcgagc gcgacttgac
     6721 cttgcccttg gcgccgctct tctcgccgga gtcggcaccg gagccgcggg ccagctcctt
     6781 catggcgccg ttcaccttgt caggctcggc gagcttcttc tggttcgcga ggaacgcgaa
     6841 gtagctatcc ggcatgtcgt cgaagtccat gacgagatcg cactcgtcac agcgacacgt
     6901 cggcggctcg tgcggaggcg ggcccatgtc gccggcttcg acgaggagga ccttctcttc
     6961 gtcgcactcg ggacagaagt acgggacgta gaagctcttg acgacaccgt tgccggtgaa
     7021 gttgttgacc aggttgatct gcgcgacgat cgcgggcgag cactcgacga gcaccgagcg
     7081 cgtgccgttg ctctcgagct tgctgagcca gttgacccag tcgcgaacgc cgcacgagtt
     7141 gatgcgctcg atctcgccga gatcgatcac ggccgtgccc gacgggatct tgtcgacgag
     7201 atcgccgagc tcgttatcct cgtcgatcac gccgccgagc ttcacgtagc tgacgtcacc
     7261 gcggtgctgg accgatgcct ggaacttttg actcatggaa gtaacggcgg tagtccgaat
     7321 tacaccacag actcgtaagc caccgctgca cagagtagcg tgcgtttttc taatgatcca
     7381 gtggggctag gtagtttgcc ttcccacatc cgctaacgct cctgccgctt caggcaggca
     7441 gcgcgagcaa gtcacggatc atctggtagt tgtcacttaa ataaaaccgc acgagatcgg
     7501 ggccgccgag gacggatccg agtgcgacgg tgtcgtcgtt gctctccaag agctcgccgg
     7561 tgagtcgcga gatcatccag atcgcggcac cgaaatcgtc gcaggcgacg aggccggctc
     7621 gtttggcgca cgcgagcatg ttgtcgatcc atgaacccgg atcgaggtcg cgcgtgccgg
     7681 cgtggcgctc gaggactttg gcggcgcgga cggtggccgt tcgcacgagc tccccggcgg
     7741 ctcccgtggg ctcgacgtcg gagatcagcg tgcgcatcag ctggccgagc tcgcggcgtt
     7801 gccgagcacc gagctggagg agggccgcat agccgccgcg gatcgcttcg aaggcgtagc
     7861 cgaacatgaa gcgaagcggc aggtccttct cttcgagcag cgagcgatcg acgacgacga
     7921 tctggcgcgg gaacgcggta acggcgagga ggccaggcac cttgtcgccg acgaagatct
     7981 ccggctcgac ctcgaacagc tggccgattt cgccggcgag ctgcaggagg cgcgcgtcga
     8041 cttgctccgc cggcagcacg ttctcgccaa gcgatggctg cgcgacgagc gcggtgatct
     8101 cctcggcgaa tgccgtccac acttcgccga gcggctcgcg tggggcggtg ttcgccatga
     8161 ggcgctcgcg gttgttctcg tcgagcgtgc ggcgcagcgg ctggctctga cgcgattggc
     8221 gcaggcgctg gacggtctgg cgatccgtct cttccgcgaa gccgaggagg tcgagcgcgg
     8281 tgagcacgcg agtcgcgcgt tcgttgtcgc ccgtgcgcgt gtagaacgac gcgaggaggc
     8341 gataggccgg cgcgtggtgg atgtcgcgct cgagcacctt gcgcagctcg ccgatcgcgc
     8401 gcgtcggatc gtcgacggcg tagatctccg cgagtgcaac gcgatcgctc gcgccatccg
     8461 gctcgacgtt caagatgccg cgatacgcct cgatcgctgc ggggcggtca cccgacgaca
     8521 ggagaatgcg cgcgagacca cgctgcagcg gcaccgcggc cctcggacca ccttgcgcca
     8581 tcgccgcgtg cgcggcttcg atcagcagcg tctcggcttg gcgctggtcg ccaccatccg
     8641 cagcgcggcg cgcgagcacg agcgcgggag gcgcgtagcc cgggtcgtat tcgatcgcgc
     8701 ggcggtactg cggcgcggct gcgcgcgcat cgcctgctgc atcgaggatg cggcccttgt
     8761 agtagtagta gcgagcgagc gcttcggcgg acagcggcat gccgggcgcg gtcgcgagat
     8821 cgatcacgcg ctcgatgaac gtacgcgctt cttggtagcg accgacgagg aagcactgct
     8881 gcgcgaggag gtagtaagcg tcttggtgcg tgggctcgat ctggatgacg tgcttgagca
     8941 ccgcgatcgc gcgcgtcgga tccatctcgc cgtcggcgtg aatattcgcc tgccgcatga
     9001 gcgccaatag acgctccgtg cccgacgcgc cggcctcgac gaacttctgg attgcgtcga
     9061 ccgcctgctt ccagcgccgc atgtccgcgt agaaatcgac gagcgcgttg agcacggtga
     9121 ggtccgtcgg cgcgagctcg agcgcggcgt gatagagctc gccggcagca tcgaggtcgc
     9181 catcgcgctc gacgatcgca gcacgcgcga cgttcacacg agcgaacagc gggcccgcgt
     9241 cgtcgcgctt cttgtagacc ttctcgagct ctttgtacgt cgcgtcgaag tcccacgcgt
     9301 cgggttggtc gcgggccagc gagccgagat cgtcgagggc ggcggtgttg aggggcttga
     9361 tctcgagcgc gacgacgaag ctgtctgcgg cgctcttgag gtcgccggcc atgcgcgcga
     9421 cgacaccgcg cttgcgatag aactcgctgc gcgtgccggg atcgccgtcg cgcatcgcct
     9481 tctgcgcgag cgacgtcgcg atcggcatcg ccttgtccca ctcgccacgc tcgaagtagt
     9541 gctcgaacag ggcctgattc gcgggcaagc agtcgggatc gacggcgagc gcgctgtcgt
     9601 aatactgcat cgcgcgatcg gggtcgttga gctgctgcgc gtagatgcgg ccgatctgcg
     9661 cgaacacgcc ggcgcgctct ttgtcgtcgg accagagctt cacttcgagc tcgagcgtct
     9721 cgatgacgcg cggccactct tcgcgtcgac gatagagatc gcgaaggccg tggactgcag
     9781 gcatgcagtt cggcgacgtc ttgatcgcag cgtcgtagta gcgaatcgcg tcgtgctcgc
     9841 ggccgaactt cgcttccatg acggagccgc acttgaagta gagcagcgcc ttcgtgttgc
     9901 ggtcgttcgt gatcttgatg aggcggcgcg tgacgtcgat gaagtcggcc cactgctcgg
     9961 tcgattcgta gatacggccg agcgcgtcga gcgcatcgcg gttctcgggc tgcacctcga
    10021 ggaccttttg gtagtgctcg gtcgccgtgt cgacgtcgcg cgcgccttct tcggagacgc
    10081 gagcgatgcg cttcatgatc tcgacggact cggcgccgcc gtgggtctgc gagtgcgcga
    10141 ggcgcgtttt caagatgtcg atgaggcccg acttgtcctg cgtctcctcg aagtacttct
    10201 cgagcgtcga cacggcgccg gcatcattcg gatcgaccgt gagcagcttg cgattgagac
    10261 gcaccgagtt gcgcacgtcg tccatgcgat ccgtcgcgag cgcggcggcg gcgcggagtg
    10321 cgtcggcttt gcgctgcgga tcgcgctgcg tctcggcgcg cttctcgtac gcctcgatga
    10381 gcggtttcca gtcgccgcgt tgcttgctga ggtcctcgag gactttcacc gcggcctgcg
    10441 gttggctgtc gacctcgacg accttttgat acgacgcgat ggcggcgtcg acttgaccga
    10501 ggaaggagag ctgcacgttg ccgcggcgcg agtacagatc accgaggcga tacgcgcggc
    10561 cttggcccgt ctcgacgtgc tggatcgcga tctcgtacat ctgcatcgca gccgcccagc
    10621 gctcggcctt gtagcaaacg cgctccatcg cggtgaacac gtcgcggttc gtcggatcgg
    10681 cgctgaatgc tgagatgtac gcagcgatcg ctccgtcgac gtcgccgcga ttcgtgcgca
    10741 gcgtcgcgac gcgagccatg agcgctgcgc gagccacgag atcttgcgtc gcttcggcgc
    10801 gcagctcgag catgcgtgcg agctctcggt cgcggccgag ctgctcgtag agtttttcga
    10861 ggcgctctgc ggcggtcgag ttcttgggat cgcgctcgag caccgattcg aacgcttgcg
    10921 cagcctcggg caggcggttg agcttgtctg cgcgcagcgc accgagctcg aggaggacgt
    10981 cggcttggcg cagcacgtcc gacgggcgaa tctgcggcag ctcgcgctcg agcgcctgcg
    11041 caagctcgcc ccagcgctcg gcggcgcgga ggaggcgcag cagatctttt tgccgctgcg
    11101 catccggcag cgacgcctgc gcgacgacga gcatgcgaat cgccgcatcg ttgtcaccca
    11161 gctcgtgctc gtacaggcga gcgagctctg ctgcgcggcg cgccttctcg tcggtggagc
    11221 cggcggcttc gagctgcttg cgacgaacct cggcgagctc ggaccactcc gcgatcttcg
    11281 cgagcgcttc ggcgagcacc tcgaggttgc gcggcgtctg cggcgcaccg cggaatgcac
    11341 gagcgagctc gtcccacagc tcgagcttgc gcgcaggcgc ttcgatgccg gcgagcgctt
    11401 cggtctgatc gggctcgatg ctgaggacgt cgaggtacgc ggcgacgacg gcttccggct
    11461 cggagtcgag cgcggtgagc agctgcgcct cctcggtgcg aagctgcatg cggcgcgcga
    11521 ggcccggctc gccggcgagc tcgctgcgca cgatctcgag cgccttgtcg aagcgacccg
    11581 cttggcgatg cacgcgcgct tgctcgcgca tgacctcgat cttgcgaacg ccggtctgcg
    11641 ctgccttgtc gagcgccgcg attgccgcgt cgcgatcgcc tcgacgttct tcgcggaccg
    11701 cgatgtcgat ccagcgcacg gcgcgttcgt cgggcgtcgt cgacagctcg acggccttgc
    11761 ggcggagctc gatcgagcgc gcccactgtt gatcgcgatc gtagagcttg gcgagcgtca
    11821 gcatcgcgtc gtcgtcgtcg gggcgctccg cgatcatgcg ctcgagcgct tggcgtgcgg
    11881 ccatgggatc gccgagatgc tcgtcgagga gctcggcgta ttcgcgagct gcggaggcgc
    11941 gggcatcgcg gctcgtgagc gacgtcgaga gctcggcgag gacctgcgcg agcatgtcgt
    12001 agcgcttgcc tgcgcgatag acggccgcga gtgcggcgcg gcagaacgaa tctttgggat
    12061 cgagatcggc ggcgcgctgc agcgtttctt ctgcctcgac gaagtcgccg cgcacgagat
    12121 cgacgatgcc gcgtgcagcc tggagcgccg atgcgcagcg catgtcggtc tcggcgagcg
    12181 cccacagcga gtacgcctcg gccagctcgt cttcgtccat cgcgcgcagc aggcgaacga
    12241 ggtgatcgta ttcggtgccg aggcggccat ccagcgcgca cgcgcgacgc cacaactcga
    12301 gtgcctttgg aatgttgccg agcttctcgg ccagctctgc ggcccaacgc aatgcgaggc
    12361 cttcgcgcgc ttcgaggcga gcgacggtgc cctgcgtcgg cgcgggatga tcgaggagat
    12421 cgagccacca ttccgcgacc ggcgtgtccg ctgcgtcgac gacggcgtgc gcgagatcgg
    12481 agagcacctg cggcgattca cctacggcgg cggcaagctc gagtgcgcgc catgcgtgag
    12541 cggcggcagc ttcgggacac tcgctgtcga cgagacgacg tcgcgctgcg agagcggcct
    12601 tcgcgtcgcc ggcggcggca gcagcccatg caccggcgag gagagcgacg cgacgcgcgg
    12661 tggcaggagc actcgcgacg ggatcatcgg cgagctgcgc ccacagcgca gaggcttccg
    12721 gatgctggcc gtcacgcgac agagccgccg caaccgcgtg gcgtgtcatg agtgcttcga
    12781 gcgcgccgcc gggaagattc gcgacgagct cggcgcggcg atcgagaagc tcgagcacgc
    12841 gctcgtcgcc ggcgcgcatc gctgcatcga tgccgacgtc gatcaggcgg agttggccag
    12901 cgcgattgac ggcggcggaa ggctgcgtgt cgcgaacgtc ggcgtcggtt ccttcgagcc
    12961 gctcgatggc gccccaacac agctgcagtg cgccgagcgc atcgcggccg cgatcgagga
    13021 cgagagcggc actcgccgcg atgtcgtgca gcgatgccga acgctcggcg gcggcgcggc
    13081 gaatctcgac gacgcggtcc cactgccggc ccgtcgcggc cttcgcgagt gcggcgagat
    13141 tcgtgacgtg ctcgcgccac ggcgccggaa tttcggcttc gagcacgcgc tcgacgagag
    13201 cagcggcgac atcggcgcgg ccatagcgga acagccacgc ctcggtgact tcgatgagca
    13261 gtgcagtcgt agtctggctc gcggggagcg actcgatgcg gccttgcgct tgcgcgagtg
    13321 cttcgggcga gtgagcggcg agggcggccg agaggcgcag gcgcggcgcg agcggatgac
    13381 tctcggcgag ctcgagatag cgaagtgcct cgtcgggctc gtgcagaccg tcccagctcg
    13441 cgaacgccgt gcgcacgttg agatcggcga gacggccgtc gggatcctcg gcggtaagca
    13501 gcgcctcggc ttcgctcgcg acgagctcga gcatcttgtg aacgagctgc gtggcgtcgg
    13561 gcgcttcgag gttcttcgtg atctcggaga cttcttcgcc gccggcccaa ccaacatcga
    13621 gctctggatc tcgctgcgcc ggaacgacgt ccgttttcgc gggagcaggc gcgatcggag
    13681 cacgcgccgg tggcggcggc ggcttcgtcg aagacccgcg aatcggaggc ccttccccca
    13741 tgaaggatcg gatcgtaccg gggatagcgc gacaccgcca gctagtggat tttccttgat
    13801 tgaccaaagc gcattcgccc cgctaacctc tgcgcccaga tgaggccgcg tgcgatctgc
    13861 atttgccgcg atgatggcgt ctgccgcgtc gtcggacatg cgctcgacag cgcgggcgtc
    13921 gaggtcgagc atctcgcgca agtgcctacg gatcccggtg aagtggcgct gtttgtcgtc
    13981 gatcgagaga ccggcgcgac gtctgagaat gaccttcgcg gagtcggtgc accggtcgtg
    14041 ttggtcggta atgacctcga agacgaggga atgatcaacc tgatgcacga ggcgccgttc
    14101 tcgcacctcg tgcgcgatcc agccgaccgc gagctgggaa tcacgtccga gaaacttgta
    14161 tctggcgacc tcttcggctt ggaaaaatac ctcgcgaagg gcgcgcgcgt tggcgagcga
    14221 gtcgtcagca cggacgtcga gaagcgcgcc gcgatgcatg aagtgatcgc gtgggccgaa
    14281 gcgatcggtg cgcgtcgtcc gatcgtgcat cgcctcgcga cggtgatcga cgagctgctc
    14341 atgaacgcgt tgcacgatgc accgcgcgag tcgaagccgg tgatctcgat cgaagacgag
    14401 ctagcgaaga aagcggtcct gcgctggggc gcggacgagc agtcgatcgc ggtgtcggtc
    14461 agcgatccgt tcggagcgtt gcggcagcgc gatgtgatcg atcacgtgcg gcgcgcgcgc
    14521 gatgagagag ggcgaccgat cgccgggacg accgaggatc cgggcgcagg cctcggcctc
    14581 tacttggtgc tcgcaaatgt cgcgtcgctg atcgtcaatg tggatccggg gcatcagacg
    14641 gaggtcgttt gcctcttcga tcgcgcccgg ccgggaacgc gcgctgtcac ctcaggcgtg
    14701 cgctcgctcc acgtcttcca gcagcactga gcgctgctgt ctcaggccgc gacggcgatc
    14761 acgttgtcga aggcctgcag ctcgtcgcac aggatggcga ggcgatccgc gggcaacgac
    14821 tcggcggccg tcgtgaacag ctggtcttcc tgccacgccg agtgaccgac gagcagctcg
    14881 cgcaggcact cgaagtgccc gctgaactcg gggtcctcga cgccggccca cacgagcttc
    14941 gcgagcaagt gcttgattgc aacgtgctcg gcgaggacct cgttcatcac gtcgcgagag
    15001 acgtgtgcgc cgatggcggg gtagagcagc tcctgctcga cggcgaggtg gtgaggcagc
    15061 ttgtcggcga ggagagcgaa cgcggccgcg tccttcgagc gagcgacgcg gtcacacagc
    15121 tcgtcgagct cctcgtgttg cgcggtcagt agctcgaggg catcacggat gggcttcatt
    15181 gcgatgccac gctgtgcagc gcgcgtgcca cgccttacag aagtcgcgcg gcggcgaatc
    15241 ctagcgggac cacgacgaac acgacgagcc acgcgaacac ccacatcacc ttcgcgcgcg
    15301 tcctcgcctc gatcgggatg ggcagcgtgg ctgcgaggcg caccatctcg atgggaggag
    15361 tgaaaaggcg gcgggcgaga gcgatcgcgt agagaccggt accggcgagt acgacaacca
    15421 gccagggtgc aggatcgtcg ggacgcgcga gcccgatgat gacgagcgtc gcgaaggaga
    15481 caaaaccgag cgcgaatgcc atcggatagc gtcggcgcat gaggcgcgcg tccttgcgat
    15541 agacgattcc gccgtcgtga cctaggagct tgccgaccgt gcgctcgagc gctgtaggag
    15601 gacgtatctc gagtgtcgcg agttgttgac gatcgagtgc agagacgtcg cgaaggacat
    15661 cgcccatgac gcgcgaagcg gagctgcgga tggcgagggc ggagaggaga gagagggcga
    15721 cgagcgtgac gagcgtcgcg agtggaggca gaacgggcgc cgcattctcg agccacgcag
    15781 cggtgaggag cgtgatgacg atgatgaccg tcgaggcgaa accgggcagt gcgccgagga
    15841 tgacggcgga accgccggcg ttgacgggtg tggcttggcg cgtgggcgcg ccggcaatcg
    15901 tggtggcgat ttcgaggacg ctgcggcgaa cgagagtggc agcccaggtt gcgacggcgg
    15961 ggaggaagaa ggcggccacg atgccgagcg aggcggagag agcgaggtgg cggaggaaga
    16021 acgttgcggg agagagagcg ccgggaggag gaccggcgat cggt
//
LOCUS       DATLBZ010000254         4012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9210046, whole genome shotgun sequence.
ACCESSION   DATLBZ010000254 DATLBZ010000000
VERSION     DATLBZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9210046"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VMZ53_13420"
     CDS             <1..270
                     /locus_tag="VMZ53_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29504.1"
                     /translation="GTPCPTPTGPPCDGAYPNYEVVVYAEDGSTVAGKATTGSDGTFS
                     LDLPPGKYVVFTQAGLQATDRQRTDVVISRDALAKVELRVDTGIR"
     gene            complement(281..2380)
                     /locus_tag="VMZ53_13425"
     CDS             complement(281..2380)
                     /locus_tag="VMZ53_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS29505.1"
                     /translation="MRAAGLITIVAALASTHSAAADDAADDDAADDAEDDVDEPIASA
                     TSTTLHRRELDHRPHLRARELLRNVPGLYTLWVGAQAPQYLARGYAAGQGADLGVLVD
                     NVPINIGSHAFAHGYADPNFLIADTISSIALHEGAYASRFGSYSVAGTLDIKTLDALP
                     KGSGAVVRISSGTELTTPLLASRLRRLRYQLVGLFSPELERGSALLAAEVGIDDGPYV
                     HPQRYRRGVVLGKWKRPLADGELATSIQFYSGRWFDSGFLSRAAIAASRLTPFSAADP
                     SQGGIAQRASASLAYEVTDANHATWHLAAYVVDSDVRLYANPTLYLRDAMNGDEIEYV
                     DQRVYYGIDAFYRRPHRLGPLRGRLRVGVQARADHADDFTWHDARRLRLSDCFGATNP
                     CTDLSPATNQVAAYLEDHLRYSKHLRFYAGVRLDEESWNVDDRDADTMLGPTSLGGTG
                     SRARIGPKAGILVSNEANDAELSLLAGAGSHSTDARAAVDRSGFGAFIRTYNAELGAK
                     LAPTDTLTAAVATWWSFVYENQVWDPTTATAYRADKATHYGLDARVTFTPTTWLATDA
                     SLSTARGTSTPDASASRPPELLQLAPRIMAHAGISVFRATGFVSLRVRAIGARATGDP
                     SLTADGHTIVDLVAAQRFRDFELGLTIDNVLDARWYEQQLASDVRTSRRVDVTRDVLV
                     TPGVPLTALVTLGYVPR"
     gene            complement(2380..3093)
                     /locus_tag="VMZ53_13430"
     CDS             complement(2380..3093)
                     /locus_tag="VMZ53_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUS29506.1"
                     /translation="MRLVAVLVLLAGCDVTLSAMDQIYSHGADHPVLCAFNVDNKNAV
                     SADSIAAGLDRAQAEHVVLHIYTHRPEGTVDESTIEQIVAGAADRGMPFVTYRDLVAG
                     TATEGLALSFDDHDIGGWHALRPMFDLYGAKVTFFISAFHVFDDNARMLLRDLFLDGH
                     SIDYHSTEHRNAETYSAEFGVERYIADDIVPDLELMRADGYDPTTFAYPFGARTAATD
                     AALLKLFPLVRASSFDCPH"
     gene            complement(3106..3402)
                     /locus_tag="VMZ53_13435"
     CDS             complement(3106..3402)
                     /locus_tag="VMZ53_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29507.1"
                     /translation="MIAVSALVTAVALTAVLIHKSHDTDDEKPVQHAKMTDAELRLER
                     MYAQAHLSARAGRCEAVTAIGKKLSVDDPAEYARYSSDHELAVCLPSLQTAALR"
     gene            complement(3708..>4012)
                     /locus_tag="VMZ53_13440"
     CDS             complement(3708..>4012)
                     /locus_tag="VMZ53_13440"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUS29508.1"
                     /translation="GHPYMAAFLAAWGLLVVGLVDNLVKPLLIRRGLETHGAIVFFSL
                     IGGLAAFGAIGLLLGPLLISFFLALLRIYHRDYTPDDSRVASVPGLRGNDVPSASQ"
BASE COUNT          676 a         1142 c         1535 g          659 t
ORIGIN      
        1 ggcacgccgt gtccgacgcc gacggggccg ccttgtgatg gtgcgtatcc gaattacgag
       61 gtcgtcgtgt acgcggagga tggcagcacc gtcgctggga aggcgacgac tggttcggac
      121 ggcacgttct cgctcgatct gccgcctggg aagtacgtcg tgtttacgca ggcgggtttg
      181 caggcgacgg atcggcagcg cacggatgtc gttatcagcc gtgatgcgct cgcgaaggtc
      241 gagcttcgcg tcgataccgg cattcggtag tagagcgggt ctagcgaggc acgtagccga
      301 gcgtcaccag cgcggtgagc ggcacgcccg gcgtgacgag cacgtcgcga gtgacgtcga
      361 cgcggcggga tgtgcggaca tcgcttgcga gttgctgctc gtaccagcgc gcgtcgagca
      421 cgttgtcgat cgtcaggccg agctcgaagt cgcggaagcg ttgggcggcg acgaggtcga
      481 cgatcgtgtg gccgtcggcg gtgagcgatg ggtcgcccgt cgcgcgtgca ccgatcgcgc
      541 gaacgcgaag cgatacgaag ccggttgcgc ggaagacgga gatgccggcg tgcgccatga
      601 tgcgcggcgc gagctggagg agctcgggcg ggcgggacgc agaggcgtcg ggggtggacg
      661 tgccgcgtgc ggtcgagagc gacgcgtcgg tcgcgagcca ggtagtcggc gtgaacgtga
      721 cgcgcgcgtc gaggccgtag tgggtcgcct tgtcggcgcg gtaggcggtg gcggtggttg
      781 gatcccagac ttggttctcg tagacgaatg accaccacgt ggcgacggcg gcggtcagcg
      841 tgtctgtcgg cgcgagcttg gcgccgagct cggcattgta tgtgcggatg aacgcgccga
      901 agccgctccg gtcgacggca gcgcgtgcgt cggtcgagtg cgagcctgcg ccggcgagga
      961 gcgagagctc ggcgtcgttg gcctcgttgc tgacgaggat gccggctttg gggccgatgc
     1021 gagcgcgcga gccggttccg ccgagcgacg tcgggcccag catcgtgtcg gcgtcgcgat
     1081 cgtcgacgtt ccacgattct tcgtcgaggc gaacgccggc gtagaagcgg aggtgcttgc
     1141 tgtagcgaag gtgatcttcg aggtaggcgg cgacttggtt ggtggctggg gagagatcgg
     1201 tgcacgggtt ggtggcgccg aagcagtcgg agaggcgcag gcgtcgcgcg tcgtgccagg
     1261 tgaagtcgtc ggcgtggtcg gcacgcgctt ggacgccgac gcgtagacgt ccgcgcagcg
     1321 gaccgaggcg gtgaggacga cgatagaagg cgtcgatgcc gtaatagacg cgctgatcga
     1381 cgtactcgat ctcgtcgccg ttcatcgcgt cgcgcaggta gagcgtcggg ttcgcgtaga
     1441 gccgaacgtc ggagtcgacg acgtacgctg cgaggtgcca cgtcgcgtgg tttgcgtccg
     1501 tgacttcgta cgcgaggctc gcgcttgcgc gttgcgcgat gccaccttgc gatggatcgg
     1561 cggcggagaa tggggtgagg cgacttgcgg cgatcgcggc acgcgagagg aagccggagt
     1621 cgaaccagcg gccggagtag aactggatcg aggtcgcgag ctcgccgtcg gcgagcgggc
     1681 gcttccactt gccgaggacg acgccgcggc ggtaccgctg cgggtggacg tacgggccat
     1741 cgtcgatgcc gacttcggcg gcgaggagtg cgctgccgcg ctcgagctcg gggctgaaga
     1801 gtccgacgag ctggtagcgg agccgccgca agcgtgaggc gagcagcggc gtggtgagct
     1861 cggtgcccga cgagatgcga acgacagcgc cgctgccttt cggcaacgcg tcgagcgtct
     1921 tgatgtcgag cgtgcccgcg accgagtagg agccgaagcg actggcgtag gcgccttcgt
     1981 gcagagcgat cgagctgatc gtgtctgcga tcaggaaatt cgggtcggcg tagccgtgcg
     2041 cgaatgcgtg actgccgatg ttgatcggca cgttgtcgac gaggacgccg aggtccgcgc
     2101 cttgcccagc ggcgtagcca cgcgctagat attgcggcgc ttgggcgccc acccacagcg
     2161 tgtagaggcc ggggacgttg cggagtagct cgcgtgcgcg caggtgcgga cggtggtcga
     2221 gctcgcgacg gtgaagcgtt gtcgaggtcg ccgatgcgat cggctcgtcg acatcgtctt
     2281 cagcgtcgtc agcagcgtcg tcgtcggcag cgtcatcggc cgcggcgcta tgcgtgctcg
     2341 cgagcgcggc gacgatcgtg atcagtccgg cggcgcgcat cagtgtggac agtcgaagct
     2401 actcgcgcgc acgagcggga atagcttcag cagcgctgca tcggtagcag cggtccgcgc
     2461 gccgaaggga tacgcgaacg tcgtcggatc gtagccgtcg gctcgcatca gctcgagatc
     2521 aggaacgatg tcatccgcga tgtagcgctc gacgccgaat tccgccgagt aggtctccgc
     2581 gtttctgtgc tccgtcgagt ggtagtcgat cgagtgaccg tcgaggaaga ggtcacgcag
     2641 gagcattcgc gcattgtcat cgaacacgtg aaacgcactg atgaagaatg tgaccttcgc
     2701 gccgtacaga tcgaacatcg gccgcagtgc gtgccagccg ccgatatcgt gatcgtcgaa
     2761 gctgagggcg aggccttcgg tggccgtgcc cgcgacgaga tcgcgatacg tgacgaacgg
     2821 catgcctcga tcagccgcgc ccgcgacgat ctgctcgatc gtgctctcgt cgacggtgcc
     2881 ctccggccga tgcgtataga tgtggagcac gacatgctcc gcttgcgcgc gatcgagacc
     2941 ggcggcgatc gagtcggcgc tgaccgcgtt cttgttgtcg acgttgaacg cgcagagcac
     3001 cggatggtcg gcaccgtgcg aatagatctg gtccatcgcc gacagcgtga cgtcgcagcc
     3061 cgcgagcaga acaaggacgg cgaccaatcg cacacggcga cgatatcagc ggagcgccgc
     3121 tgtctgcagg ctcggaaggc agacagcgag ctcgtgatcg gaagagtagc gcgcgtattc
     3181 ggcggggtcg tcgaccgaga gcttcttgcc gatcgccgtg acggcctcgc agcggccagc
     3241 gcgcgccgag agatgcgctt gcgcgtacat gcgttcgagg cggagctccg cgtccgtcat
     3301 ctttgcgtgc tggacgggct tctcgtcgtc ggtgtcatgg ctcttgtgga tgagaacagc
     3361 ggtgagcgcg acggcggtga cgagcgcgct gacagcgatc acggcaccgg tgtaatcggc
     3421 gctatcgtgc gaagactgcg acgacgacga ggagtcaggc cagccctgtg tagtcgtcgt
     3481 ttgcggctcg ccgcagatgg gaccgacgca ggtctgtgtg gtggtggtcg tggcgcagcc
     3541 agcgaggagc gaggaggaga tgagggcgat tccaactagg cgacggatca tgcccggcca
     3601 tagagcaacg cctataccgc ggcgcgtcgc gtgtgggatt cgcggcttag gccgtgcgcg
     3661 cgcgtcgaaa agctgacgag ctgatgagct gacggacaga ccggcagcta ttggctggca
     3721 gaaggcacgt cgttgccacg caggccgggc accgacgcga cgcgcgagtc gtcgggcgtg
     3781 taatcgcggt gatagatgcg caggagcgcg aggaagaacg agatgagtag cgggccgagg
     3841 agaaggccga tcgcgccgaa cgccgctagg ccaccgatca acgagaagaa gacaatcgct
     3901 ccgtgcgtct cgagaccgcg gcggatgaga agtggcttga ccaagttgtc gacgaggccg
     3961 acgacgagaa ggccccacgc cgcgaggaat gcagccatgt agggatggcc cg
//
LOCUS       DATLBZ010000255         5796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2511896, whole genome shotgun sequence.
ACCESSION   DATLBZ010000255 DATLBZ010000000
VERSION     DATLBZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2511896"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VMZ53_13445"
     CDS             <1..588
                     /locus_tag="VMZ53_13445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HUS29509.1"
                     /translation="VLRDADFVKIGAAIFKFLSGSGIEASYHEEIYKMTIVDALTGAH
                     NKRYFLEFLEREIARCARYRRPLSLLMFDIDHFKQINDKHGHLTGDYVLREMSRRLLG
                     RVRREELLARYGGEEFAAVLPETDITGARTFGEQIRRLVGDQPFEYEGDKFPVTVSVG
                     VACVEGEDVDVTNFIKIADDNLYRAKREGRNRVIG"
     gene            633..4124
                     /locus_tag="VMZ53_13450"
     CDS             633..4124
                     /locus_tag="VMZ53_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS29510.1"
                     /translation="MGDQRHDAHAEELLAHSAWLRALALRLSRDADDADDLVQETWLA
                     SLRRSPDEREKVRPWLSKILLNAFRMRLRGDTRRSAREEASHIVEDGPAADVLVARAE
                     MQRMLVDLVLKLDEPYRSTVLLHFSEGMSLADIARSQDVPQGTVRWRLKFALDRLRVA
                     LDVEKGGRKQWAVPLLAFSKGFLVAQKTSKAVAIAALLLLLLVVGGVILYRRGSHPPG
                     SAESGSSHGGGGGGALISGHGGTTSSSGDSDEALPRWLAQPGVAARRIAGRVTFNGTP
                     VAGAAVELASVASEAGLVAAPRKTTDASGSFDFGTQLAMEWSVRASSPGKTSATIELD
                     LRDPTSRPAPDHLELRLGTCSAAIFGNVRDASGGPIVGARLLHLNVDGPAMGPTGSTS
                     VPGGPSVITDDKGGYELCAEAKWPGWVSVDVSADGYGAIVFTGLVPGRMRVDFALVPE
                     ATINGRVVDAETGQPVPHAYVFVPRGAPGSETTAQRATFSDDAGKFKLDRVAPGPHVV
                     FAEGAGYVSSSRGVPVTIDAGQTSAEIVIRLDHGSTIRGVVRKDEKPVAGAHVSFVGT
                     DDVWRRPIALSQSDGSFVLTSVPRGTIQFTALPYDVTTPETFLVSLPEHDGVVLDVKE
                     RGAIVGHVTRDTKPVPGAHLFINGPNERELPPVIADADGHYEVHGLRAGPWTVAASSE
                     RDGAFGTEPKAVDVRLGKTSEVNIDLAFAASISGKVVDQTGAPVPGVAVLFRHTQMDD
                     AGWAVTSLDGTFRAAMMTGGGQYTPTVRLRPLSMLALRPASGADFPLITVSDGGSAVT
                     GIVLAVKIDHMTISGKVVDAAGGPVPDVRVVAELLEGSGEPLFWRGLQDPAATTDVEG
                     KFAIDDLIDGTYALRARSSTGSETTQLGVRAGTTGLSIVLAEPGVIEGTLAGFKTQPQ
                     ISAIREHSTMAPIFASPQGSAFVLRDLTPGTYVVSARSASEGATATVVVHAGTTAHAT
                     LTSKGSGIVAGHVIEFRSRKPVEGITCSALPRIENTTPIGTSGGGVATNNGGAFLIPA
                     APAGEVVIQCEGLRGLYSDGLRAVTLQPGKRLDVDVPVVGVAQPTPISGFGCDFVDGA
                     LVPTLSRIEPGGPAAVAGLQNGDVIVSVDGASITELSPQGVWILITNRTTGSKVKLGA
                     MRAGKSVTAEVVLGPLR"
     gene            complement(4126..5202)
                     /locus_tag="VMZ53_13455"
     CDS             complement(4126..5202)
                     /locus_tag="VMZ53_13455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUS29511.1"
                     /translation="MPAKGKPLITANQVTLARLIPMPLLAWLIYHGAKLGYEGNWYMW
                     SALIAGTVIGCTDWVDGMLARKYGPTVLGGLLDPIADKVFIAFAYTPFADPAINLVPW
                     WACALMFTREFFITALRSAYEQRDLSLKTSYIAKAKTWTQMQGIGVMLLFPLLENKDI
                     LMGFFVVGAAIPIIAMAVLWFTKKKFWRGALVMTGSFLAMIAVFTWDERYAPRALMVG
                     ILAITWISGIDYIVAGWTQLRGRGDFRRADGVRLIGSLSLPVLLFAVLVYTDAPRWCI
                     FTILAFELAVGGLDNLLSHHKVATLALAWGGRVLGVSILLGGALLLKDQAAALTIVAT
                     CISVFGVTAEFYRGRAYYIDHLME"
     gene            complement(5248..>5796)
                     /locus_tag="VMZ53_13460"
     CDS             complement(5248..>5796)
                     /locus_tag="VMZ53_13460"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HUS29512.1"
                     /translation="ALMSADTGGPVYPIAAPGVSMRIEADQLVARAAIYNGDAVERHG
                     VPTHLAGDSLAIGEVQLAGLVQLGAWHHTETGNGYYAIVDHQLEPLLGAFARVAIAPD
                     QPVSRYIDTGVRLRMGPWRPRDFASAGVAFARTNAGTQTLLELTYQILIRGWLTVQPD
                     AQLVLQHDKGSVVMAVRAVIAL"
BASE COUNT         1004 a         1854 c         1968 g          970 t
ORIGIN      
        1 gtcctgcgcg atgccgactt cgtgaagatc ggcgctgcca tcttcaagtt cctctcgggc
       61 tcgggcatcg aggcgagcta ccacgaagag atttacaaga tgacgatcgt ggacgctctg
      121 acgggcgccc acaacaagcg ctacttcctc gaattcctcg agcgcgagat tgctcgctgc
      181 gcgcgctatc gccgcccgct ctcgctcttg atgttcgaca tcgatcactt caagcagatc
      241 aatgacaagc acggtcacct cacgggcgac tacgtgctgc gcgagatgtc gcgccggctc
      301 ctcggacgcg tgcgtcgtga agagctgctc gctcgctacg gcggtgaaga attcgcggcg
      361 gtgttgcccg agacggacat cacgggcgcg cgtacgttcg gtgagcagat tcgccggctc
      421 gtgggcgatc agccgttcga gtacgaaggc gacaaattcc cggtgacggt ctccgtcggc
      481 gttgcgtgcg tcgagggcga ggacgtcgac gtgacgaact tcatcaagat tgcggacgac
      541 aacctgtatc gcgcgaagcg cgagggtcgt aaccgcgtca tcggttagat ccgacccaac
      601 ggatcgcggt cgggtggcat cgtgtaaggg cgatgggcga ccagcgtcac gatgcacatg
      661 cagaggagct cctcgcgcac tcggcgtggc ttcgtgcact ggcgttgcgt ctgtcgcgcg
      721 atgcggatga cgcggacgac ctcgtccagg aaacgtggct agcttccctg cgccgctcgc
      781 ccgacgagcg cgagaaggtt cggccgtggc tgtcgaagat cctgctcaac gcgtttcgca
      841 tgcggctgcg tggtgacact cgccgttccg cgcgcgaaga ggcgagccat atcgtcgaag
      901 atggacccgc cgcggatgtc ttggtcgcac gcgccgagat gcagcgcatg ctcgtcgatc
      961 tcgtcctgaa gctcgacgag ccgtaccgct cgacggtcct gctgcacttc tccgaaggca
     1021 tgtcgctcgc agacatcgcg cggagccagg acgttccgca agggacggtg cgatggcggc
     1081 tcaagttcgc gctcgatcgt ctgcgcgtcg cactcgatgt cgagaagggc ggtcgcaagc
     1141 aatgggccgt gccgctgctc gcattctcga aaggatttct cgtggcgcag aaaacatcca
     1201 aggcggtcgc gatcgcggcg ctgctcctgc tgttgctcgt ggtcggcgga gtcatcttgt
     1261 atcgacgagg ttcgcacccg ccaggttccg cggagagcgg gagctcgcac ggtggtggtg
     1321 gtggcggcgc gttgatatcg gggcacgggg gcacgacaag ctcgagtggt gacagcgacg
     1381 aggcgctccc tcgatggctc gcgcagcctg gtgttgccgc gcgccgcatc gcaggtcgcg
     1441 tgacgttcaa cggaacgcct gttgccggcg cagcggtcga gctcgcgagc gttgcgtcgg
     1501 aagccggctt ggtggcggct cctcgcaaaa cgaccgacgc atcgggctcg ttcgatttcg
     1561 gtacccagct cgcaatggaa tggagcgtgc gcgcttcgtc gccgggcaag acgagtgcaa
     1621 cgatcgagct cgatctgcgc gatccgacgt cgcgaccggc gcccgaccat ctcgagctgc
     1681 gtctcgggac gtgcagcgcg gcgatcttcg gcaacgtgcg cgacgcgtcg ggcggcccga
     1741 ttgtcggcgc tcgcttgctg catctgaatg tcgacggccc tgcgatgggg cccacaggtt
     1801 cgacgagcgt tccgggcggc ccatcggtaa tcacggatga caaaggtggc tacgagctgt
     1861 gcgccgaagc gaagtggccg ggctgggtct ccgtcgacgt ttctgcggat ggctacggcg
     1921 cgatcgtgtt caccggcctc gtgcccggac gcatgcgcgt cgatttcgca ctcgtcccag
     1981 aagcgacgat caacgggcgc gtcgtcgacg ccgaaaccgg acagccggtg ccgcacgcat
     2041 acgtgttcgt gccacgcggt gcgccaggca gcgagaccac cgcgcaacgc gccaccttca
     2101 gcgacgacgc tggcaagttc aagctcgacc gcgtcgctcc aggaccgcac gtcgtgttcg
     2161 cggaaggtgc gggctacgtc tcgtcatcgc gcggagtgcc ggtgacgatc gatgctggcc
     2221 agacgagcgc cgaaatcgtg attcgcttgg accatggctc gacgatccgc ggcgtcgttc
     2281 ggaaggacga gaagcctgtc gccggtgcac acgtgtcgtt tgtcggcacc gatgatgtgt
     2341 ggcggcgacc gatcgcactc tcgcagagcg acggaagctt cgtcctcacg agcgtgccgc
     2401 gcggcacgat ccaattcacc gcactgccgt acgacgtgac gacgcccgag acgtttctgg
     2461 tctcgctccc cgagcacgat ggcgtcgttc tcgacgtcaa agagcgaggc gcgatcgtcg
     2521 gtcacgtgac gcgcgacacg aagcccgtgc ccggcgcgca tctcttcatc aacggaccga
     2581 acgagcgcga gctaccgccg gtgatcgcgg acgccgacgg tcactacgaa gtgcacggcc
     2641 tccgtgcggg gccatggacg gtcgctgcgt cgagcgagcg cgacggtgcg ttcggaaccg
     2701 agcccaaagc cgtcgacgtg cggctcggca agacgtccga ggtcaacatc gaccttgcgt
     2761 tcgcagcatc gatctcgggc aaggtcgtcg atcagaccgg agcgccggta cctggtgtcg
     2821 cggtgctgtt ccgccatacc caaatggatg acgctggctg ggcggtcacg tccctcgacg
     2881 gaacctttcg cgctgcgatg atgaccggcg gcggccagta cacgccgacg gtgcgcttgc
     2941 gcccgctctc gatgctggcg ctgcggcccg catccggcgc cgactttccg ttgatcacgg
     3001 tgagcgacgg cgggtcggcg gtgacgggca tcgtcctcgc ggtcaagatc gatcacatga
     3061 cgatcagcgg caaggtcgtc gacgccgcag gtggtcccgt tcccgacgta cgcgtcgttg
     3121 ccgagctcct cgagggcagc ggtgagccgc tgttctggcg cgggcttcaa gatccggcgg
     3181 cgaccacgga cgtcgaaggt aaattcgcga tcgacgatct catcgatggc acgtacgcgc
     3241 ttcgtgcccg ctcgtcgacg ggtagcgaga ccacacagct cggcgtccgc gcgggtacga
     3301 ccgggctctc gatcgtgctc gcagaaccgg gtgtcatcga aggcacgctc gcgggcttca
     3361 agacgcagcc ccagatcagc gcgatccgcg agcactcgac gatggcgcca atcttcgcgt
     3421 cgccgcaagg tagcgcgttc gtgctgcgcg acctgacgcc gggaacgtac gtggtctccg
     3481 cacggagcgc gagcgagggc gcaaccgcga cggtcgtggt gcacgcaggc acgacggcgc
     3541 atgcaacgct cacgagcaaa ggctcgggaa tcgtcgctgg tcacgtgatc gaatttcgat
     3601 cacgcaagcc cgtcgagggc atcacgtgca gcgcgctgcc gcgcatcgag aacacgacgc
     3661 cgattggaac ttccggtggc ggtgttgcaa cgaacaatgg aggcgcgttc ctgattcccg
     3721 cagcgccggc gggcgaggtc gtgatccaat gtgaaggtct gcgaggcctg tactccgacg
     3781 gccttcgtgc cgtgactcta cagcccggca agcgcctcga cgtcgatgtc cccgtggttg
     3841 gcgtcgctca gccgacaccg atctccggct tcggctgcga cttcgtcgac ggtgcgctcg
     3901 tgccaacgct ctcgcggatc gagcccggcg gtccggcggc ggtcgcggga ctccagaacg
     3961 gagatgtcat cgtgtccgtc gacggagcaa gcatcaccga gctcagcccg cagggcgtgt
     4021 ggattctgat cacgaaccgc accacaggct cgaaggtcaa gctcggcgcg atgcgtgcgg
     4081 gtaagagcgt gaccgccgag gtcgtcctcg gcccgttgcg ctagctcatt ccatcaagtg
     4141 atcgatgtag tacgcgcggc ctcggtagaa ctccgccgtc acgccgaaca cgctgatgca
     4201 cgtcgcgacg atcgtgagcg cagcggcctg atcctttagc agcagcgcgc cgccgaggag
     4261 gatgctgacg ccgagcacgc gcccacccca tgccagcgcg agcgtggcga ccttgtggtg
     4321 cgagagcaga ttgtcgaggc cgccgaccgc gagctcgaac gcgaggatcg tgaagatgca
     4381 ccagcgcggc gcatcggtgt agacgagcac cgcgaacagg agcacgggca gcgagagcga
     4441 gccgatgagg cgtacaccgt cggcgcggcg gaagtcgcca cggccgcgca gctgcgtcca
     4501 acccgcgacg atgtagtcga tgccgctgat ccacgtgatc gcgaggatgc cgaccatcag
     4561 cgcgcgcggc gcatatcgct cgtcccaggt gaagaccgcg atcatcgcga ggaacgagcc
     4621 ggtcatgacg agcgcgccgc gccagaactt cttcttcgtg aaccagagca ctgccatcgc
     4681 gatgatcggg atggccgcgc cgacgacgaa gaagcccatc aagatgtctt tgttctcgag
     4741 cagcgggaat agcagcatca cgccgatgcc ctgcatctgc gtccacgtct tggccttcgc
     4801 gatgtagctg gtcttcagcg agaggtcgcg ttgctcgtac gccgagcgca gcgcggtgat
     4861 gaagaactcg cgcgtgaaca tgagcgcaca tgcccaccac gggacgagat tgatcgccgg
     4921 gtccgcgaac ggcgtgtacg cgaacgcgat gaagactttg tcggcgatcg gatcgagcag
     4981 gccgccgagc acggtcgggc cgtacttgcg cgcgagcatg ccgtcgaccc agtcggtgca
     5041 accgatcacc gtgccggcga tcagcgcgct ccacatgtac cagttgcctt cgtagccgag
     5101 cttcgccccg tgatagatga gccacgccag aagaggcatg gggatcaggc gcgccagcgt
     5161 gacctggttt gccgtgatga ggggcttgcc cttggcaggc acgggcgcac cctatcatca
     5221 agtccaacgc cctcacaccc ccaacgatta caacgcgatc actgcccgga ctgccatcac
     5281 caccgagcct ttgtcgtgct gcaggacgag ctgcgcgtcg ggctgaacgg tgagccagcc
     5341 gcgaatcagg atctgatacg tgagctccag cagcgtctgg gtgcccgcgt tcgtgcgtgc
     5401 gaaggcgact ccggcgctgg cgaagtcgcg tggacgccac ggacccatgc gcaggcgaac
     5461 gcccgtgtcg atgtagcgcg agaccggctg atcgggcgcg atggcgacgc gtgcgaatgc
     5521 gccgagcagc ggctcgagct gatgatcgac gatcgcgtag tagccgttgc ccgtctcggt
     5581 gtggtgccac gcgccgagct gcacgaggcc agcgagctga acctcgccga tcgcgagcga
     5641 gtcaccggca aggtgcgtgg gaacgccgtg tcgttcgacg gcatcgccgt tgtagatcgc
     5701 agcgcgtgcg acgagctgat cggcttcgat gcgcatgctc acgccgggcg ccgcgatcgg
     5761 atagacgggg ccgccggtat ccgccgacat gagcgc
//
LOCUS       DATLBZ010000256         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2512702, whole genome shotgun sequence.
ACCESSION   DATLBZ010000256 DATLBZ010000000
VERSION     DATLBZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2512702"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VMZ53_13465"
     CDS             complement(<1..578)
                     /locus_tag="VMZ53_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HUS29513.1"
                     /translation="MARAKALTAHDPLRRWTASDANDTYNIPRWGCGYFGVNEQGNLI
                     VTPQGEGNGAIDMKELIDELVARGIQLPILLRFSDILKARIELLCASFNSAIKEYGYS
                     GQYRGVYPIKVNQNRTVVEEIIEYGRPFPYGLEAGSKPELLAIMAIHQDDEALIVCNG
                     YKDEEYIETALLASKMGKTVILVVEKPTELDN"
     gene            complement(629..1810)
                     /locus_tag="VMZ53_13470"
     CDS             complement(629..1810)
                     /locus_tag="VMZ53_13470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29514.1"
                     /translation="MAATDVCGSRLGNYELVRLLAQGGMADIYLARQHGLNRLCAVKV
                     LNGQRARDAESCALFMDEARLAGLLTHANLASVYDVAAEDGVHYLAMEYVQGADLREI
                     LARCEQKQMAVTFESALTIVAGAASALDYAHRRCDADGRPLHLVHRDVSLSNIMVGHD
                     GSVKVIDFGIAISTASMHHTNPGIVRGKASYMSPEQCLGDAVDLRTDVFALGIVLYEL
                     TTGRRCFSGNSDFERMLAVVQGEWTAPRDFIPEFPESLEQVIKTALALDPDHRYSSAA
                     ALIEALEAVAMLEGWTLGTAATVELMQSLYATQQQTWATAANDDETVVTPPPAPPTKI
                     MTSPRRFARGTESDVYEPVVVVEVPEAANVPAANDWQDEAPTRGRRSMPRMWSALSLA
                     A"
     gene            2007..>3286
                     /locus_tag="VMZ53_13475"
     CDS             2007..>3286
                     /locus_tag="VMZ53_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29515.1"
                     /translation="MANEVAKDTPAAAIIAEEERLFGQVQARVAMGEEDDGRPTFGAS
                     DLDADLLSLRDQISEARAEDLPPLVEQMTRLAALRTRLGGGRSLPVDITSPYFAHLRL
                     REGGKSRDVMVGKRGFIDRASNVQIVDWRNAPVSRIYYRSEEGDDYEEEIAGRRVEGV
                     VEARRNVSIAKGNLRRIGTPQGTYLKSASGHWVQAVGQIAPVLHGGMGKAARPVAAPG
                     QKGKLGIHHGAAARADKSLPEIAALIDKEQFELITQPSSGFVVIQGGAGSGKTTVALH
                     RVAYLNFQDPRRFNPKNCLFVVPSQALVRYVSGVLPALGVSGVPVITYTGWARSTRMR
                     CYPDAPTKYNADPPEQVSRVKKHPAMLQILDRWVEAQAEKIGTELAAVCADAVEPWNA
                     KVNRPLVPRLSSVASWTKKQTTLDPTVRVALEGFV"
BASE COUNT          557 a         1087 c         1072 g          570 t
ORIGIN      
        1 atgttgtcga gctcggtcgg cttctcgacg acgaggatga cggtctttcc catcttcgac
       61 gcgagcagcg cggtctcgat gtactcctcg tccttgtagc cgttgcagac gatgagcgct
      121 tcgtcatctt ggtggatcgc catgatggcg agaagctcgg gcttgctgcc ggcttcgagg
      181 ccgtagggga acgggcggcc gtactcgatg atctcttcga cgaccgtgcg gttctggttg
      241 accttgatcg gatacacgcc gcggtactgg ccggagtagc cgtactcctt gatcgcgctg
      301 ttgaacgacg cgcacagcag ctcgatgcgc gccttcaaga tgtcgctgaa gcgcagcaga
      361 atcggcagct ggatgccgcg cgcgacgagc tcgtcgatga gctccttcat gtcgatcgcg
      421 ccgttgcctt cgccctgcgg cgtgacgatc aggttgccct gctcgttcac tccgaagtag
      481 ccacaacccc accgcgggat gttgtaggtg tcgttggcat cggaggcggt ccaccggcgc
      541 aacggatcgt gtgcggttag agctttcgcg cgtgccacat cacggtgtta cggccaattg
      601 ccccccaccg caatggggat ctccatgatc acgcggcgag ggacagcgcc gaccacatcc
      661 gcggcatcga gcgacggccg cgcgttggcg cctcatcttg ccaatcgttc gctgccggaa
      721 cgttcgcagc ctcgggaacc tcgacgacaa cgacgggctc gtagacgtcg ctctcggtgc
      781 cacgcgcgaa gcggcgcggg ctcgtcatga tcttcgtcgg cggagccggc ggaggcgtca
      841 cgaccgtctc gtcgtcgttc gcggcggtcg cccacgtctg ctgctgcgtg gcgtacagcg
      901 actgcatgag ctcgaccgtt gccgcggttc cgagcgtcca gccttcgagc atcgccaccg
      961 cttcgagcgc ctcgatcagc gccgcagccg acgagtaacg gtggtccgga tcgagcgcga
     1021 gcgccgtctt gatgacctgc tcgagcgact cgggaaactc gggaatgaag tcgcgcggcg
     1081 cggtccactc gccttgcacg accgcgagca tgcgctcgaa gtcgctgttg cccgagaagc
     1141 agcggcggcc cgtcgtcagc tcgtagagca cgatgccaag cgcgaacacg tcggtgcgca
     1201 gatcgaccgc atcaccgagg cactgctcgg gagacatgta gctcgccttg ccgcgcacga
     1261 tgcccgggtt cgtgtggtgc atcgatgccg tggagatcgc gataccgaag tcgatgacct
     1321 tcaccgagcc gtcgtgaccg accatgatgt tcgacagcga cacgtcgcga tgcacgaggt
     1381 gcagcgggcg gccgtcggcg tcgcagcggc ggtgcgcgta gtcgagtgcc gacgccgcac
     1441 cggcgacgat cgtcagcgcc gattcgaatg tcacggccat ctgcttctgc tcgcagcgcg
     1501 cgaggatctc gcggaggtcc gcaccttgga cgtactccat cgcgaggtag tgcacgccgt
     1561 cctctgccgc cacgtcatag acgctcgcga ggttcgcgtg cgtgagcagg cccgcgaggc
     1621 gcgcctcgtc catgaacagt gcgcacgact cggcgtcgcg agcgcgctgg ccattgagca
     1681 ccttcaccgc gcacagccgg ttgaggccgt gctgacgagc cagatagatg tccgccatcc
     1741 cgccctgcgc gagaagacga accagctcgt agttgccgag ccgagacccg cagacgtcgg
     1801 ttgccgccat cgcacatcct acgaacgagc cggtcgatcg gttccgattg actgagtgcg
     1861 tcgggagtgt gtggtgcatg tgcccttcta ttcgccaaga ggagggctct ggatgcacgt
     1921 cgcagcgaaa tcgtaagcgc tcgattccag agggttactt acgacttgtc atggccaagc
     1981 tttcggatag attcgccgtt tcccaaatgg ccaacgaagt ggcgaaagac acacccgccg
     2041 ccgcgatcat cgcggaggag gaacggctat tcgggcaagt gcaggcgcgc gtcgcgatgg
     2101 gcgaggagga cgacggccgc ccgactttcg gcgcgtccga cctcgatgcc gatctgctct
     2161 cgctgcgcga tcagatctcg gaagcacgcg cggaagatct cccgccgctc gtcgagcaga
     2221 tgacgcgcct cgcagcactg cgcacgcgcc tcggtggcgg tcgctcgctg ccagtcgaca
     2281 tcacgtcgcc atacttcgcg catctgcggc ttcgcgaggg cggcaagagc cgcgacgtca
     2341 tggttggcaa gcgcggcttc atcgatcgcg cgagcaacgt gcagatcgtc gattggcgca
     2401 acgcgccggt ctcgcgcatc tactaccgct ccgaagaagg cgacgactac gaagaagaga
     2461 tcgccgggcg ccgcgtcgaa ggcgtcgtcg aagcacgccg caatgtctcc atcgcgaagg
     2521 gcaaccttcg tcgcatcggc acaccgcagg gcacgtatct caagagcgcg tcgggccatt
     2581 gggtccaagc ggtcggccag atcgcgccgg tgctccacgg cggcatgggc aaagccgcgc
     2641 gccccgtcgc tgcgcccggc cagaagggca agctcggcat tcaccatggt gccgcggcac
     2701 gcgccgataa gtcgctgccc gaaatcgccg cgctgatcga caaggagcag ttcgagctga
     2761 tcacgcagcc gtcgagtggc ttcgtcgtga tccaaggcgg cgccggctcg ggcaagacca
     2821 ccgtcgcgct tcaccgcgtc gcgtacctca acttccagga tccgcgccgc ttcaatccga
     2881 agaactgcct gttcgtcgtc ccgtcgcagg cgctcgttcg ttacgtctcg ggcgtgttgc
     2941 ccgcgctcgg cgtctcgggc gttccggtca tcacgtacac gggctgggct cgctcgacgc
     3001 gcatgcgctg ctatcccgac gcaccgacga agtacaacgc ggatccgccc gagcaagtct
     3061 cgcgcgtgaa gaagcatccc gcgatgctcc agatcctcga ccgctgggtc gaagcgcaag
     3121 cggagaagat cggcaccgag ctcgcggccg tctgcgccga cgcggtcgag ccgtggaacg
     3181 cgaaggtcaa ccgcccgctc gtgccgcgcc tgtcgtcggt tgcctcgtgg accaagaagc
     3241 agacgacact cgatccgacg gtgcgcgtcg cgctcgaagg cttcgt
//
LOCUS       DATLBZ010000257        10764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3994904, whole genome shotgun sequence.
ACCESSION   DATLBZ010000257 DATLBZ010000000
VERSION     DATLBZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3994904"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VMZ53_13480"
     CDS             complement(<1..350)
                     /locus_tag="VMZ53_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29516.1"
                     /translation="MRLWMLLISLAGCSGVFSDPGAGELVTYGTRPTAIAMSGTDLYF
                     VSDSSASQDFLLRVPKFPGGSAQLVAAGDVITAITADAHGVYWLETSGGQTRLLAWSQ
                     GDSAPRVLGSNPNFA"
     gene            complement(398..2035)
                     /locus_tag="VMZ53_13485"
     CDS             complement(398..2035)
                     /locus_tag="VMZ53_13485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUS29517.1"
                     /translation="MTRQRLVGYGLALLTFFVVLANQREVGIARDEVVYFNAGSRYAE
                     WWDRAFTGKGVVSKKGITDYWGGIGGGNNAEHPPLAKTLIGASHKLFHDKLHILDELT
                     AFRLPGALFAALVVWLVFSMTLAIWGFAEAGLAAACMLLMPRALFHEGLACFDAPIMA
                     MWFLTIYWYWRCLDGRRFPVEVGAAFGLALATKHNAILLPFALGLHYVIIGAVAGGLR
                     GAFLHRWRVIVSMLILGPVVLFYLWPWLWIAPIKHTKEWLSFHLTHVHYNFEYLGHNW
                     NAPRFPWHVALVTTLFTVPIGTLAGALTGIGVWIGRWRTKTSADRERAPALLLGLSAA
                     ASMGPFFLGTTPIFGAEKHWMPALPTLCIAAGVGGIWAARQAARYLETFRSIGEATAR
                     KAALSLVAGTMILAAATETIDAQPYGLTWYNSLAGGAPGGADRGMNRQFWGVSAKGVL
                     PFLQWMPPGPVYSHDASPAWGWYMRLGMLSRGYPDAGHEYDGIQRSQYALVIHEKHFN
                     RHDYLIWKSYGTVQPVFVLRSDGVPIVSVYKRPPKAP"
     gene            complement(2032..2829)
                     /locus_tag="VMZ53_13490"
     CDS             complement(2032..2829)
                     /locus_tag="VMZ53_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29518.1"
                     /translation="MPTRRPSPVAVVPALLLIVIATWEVCSTPRLAASVPGDDAWDRA
                     EKLVRAGYQPGDLIVFAPSWNDPVGRLHLGDLIPIDVAARMDASKFGRIWEVSIEGEH
                     APEVAGLRPVKTATVAGVTVNLYTREPAIVVSDLKSAMATMKVEGGGRPSLELAEVGF
                     APHECLQIVPAPNHPVRITFPQLQLGSKLVVGVGLADVFTRRDIRAPATLAIEIAGKQ
                     VAATSAGVDDGWVKLTAATAPGPSDVTFIVSANAPQRLVCFMAEAHQ"
     gene            2864..4192
                     /locus_tag="VMZ53_13495"
     CDS             2864..4192
                     /locus_tag="VMZ53_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29519.1"
                     /translation="MRLYALLFVIGLVAYGGLAAKRVFSPSAAPQFVVQADSWLHGKL
                     SVTPTKTDADWSKVETVELRDRPGELVQGRRMITRPFFHPLRGAEIPNAQIGGTREVT
                     WYMSFPPFPTVIMLPSAIISGRDGNDVLPTILFAALILPLSLLVLRRLAAAQLSQRSL
                     GDDLWLVAMLAFGSVMFFSAVQGSVWYTAHVVGVVLALIYAWGSIEAKRPIIAGLALG
                     AAALTRTPMAFMFPLFLAEAWRMAGGDRRTFVRGVVPFAIPVAAFAIAGMAYNIVRFH
                     SPTEFGHTYLDVRQQAQIEQWGLASYHYLTRNLAVAFTLLPELPGHWPWIQISGHGLA
                     LWLTTPILLYALWPTQKPAIHRALWITVACVAIPSLFYQNSGWVQFGYRFSLDYMVFL
                     MMLLAIGGRRFGWLARGLIIVGILINLFGAYTFNRSGQYYHYNYDVVVAH"
     gene            4205..6625
                     /locus_tag="VMZ53_13500"
     CDS             4205..6625
                     /locus_tag="VMZ53_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29520.1"
                     /translation="MKLAVLVCALAVSACIQSGLVPCGDRLCARDAMCIAGSVCANAD
                     QLSACAGADEGTVCTLVSGTVGRCTLGVCVPSGCGNMRMDPGEVCDDGNVTAGDGCSG
                     TCDSDEMCGNGVLDAIKGESCDCGADDTRVPQSCTGPNSDDPSSPCTTGCRLRRCGDG
                     EVAGPEQCDGTMMPDGIACTSYGFYTGTVACSALCQFDIAGCSERCGDSIVQTAYEVC
                     DGTPPADQTCLDFGYDLGWLGCTGVCTPGLADCDRLGWARAHQRIATATDVIPRGNRL
                     LVRFFDGKVSLLIDDVAKDPTGTFLATASTETDSYAIAQTSVMHHATDWQALPIAPPW
                     TAQPTYAFASSSLGLFVVVEGDANAYRWNGTQWLTYAIPGGAIAKPAIGGSRTRPYIY
                     DPSGTSIAMWNGAGFAAVTPPANTTTVHGVAETSTALYVATEDGLFGRAIVSSGFGDQ
                     GIPALQILANSNDGVDVLTTAGVILRLGNGALQFSAPPGTAGISEEGGRIFVTGVPSG
                     VFAMLKGTWQANDPPAATPQTYTYIHGSGPLKDDVYLVTGPATWELQPGRWVNITVPP
                     GTVDAIRTEDSLVSIDTSLTISKATGSTTKSFSVITSPKMLFYPPTSGKVFIAGDKGA
                     AILNTTSGVLDEVNLNADLKAIHGASATNVFAVGSDRTTGVGVVYRYDGINWKPIDVP
                     LSPFPPLRDVFVSPTGKAYAVTRNTVFFGSDKPSDWTALAVPGADLVSISGTKDDDFF
                     VAGPSQFGFPFSGLAHWDGTHFYPVRLPATAAPRDVFVSIGAVYVLLTDATANRDNVY
                     ALVRATRF"
     gene            complement(6626..8854)
                     /locus_tag="VMZ53_13505"
     CDS             complement(6626..8854)
                     /locus_tag="VMZ53_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29521.1"
                     /translation="MSRAGTDLDRPADLPERYVLREVIGTGGMGRVYRAHDTTLGRDV
                     AVKVIEHIAVTGDDTQPRDRFIREARAAARLAHPNIVAVHDANPEAGWLVMDLISGES
                     LREIAARGPSSPVRVRGFAEQVLGALDAAHSSGVIHRDIKPSNIIVDARGKVTLVDFG
                     VARLVDAEVTRTGESLGTPAYMAPEQLRGAKVDARTDLYGLAATLYELVSGERMVAFE
                     TPSDAAIASVRLKCDPEYGLGELISRCLRAAPEDRIGSAREALAVLTDSKRPRSRRKT
                     AAVLAGLLALGAGCGAVAWTRMHHEKPAEDPRRLELFALTQSGDHRRAELLLQQYVRA
                     HPNDPDARMMMLLTSWWTHGVFPSNPVEGRDKLRPVHRDMIEGLQLVASRRESQAIAY
                     LEDAEHRYPSSVEIQYALGEARWHGQQIERGVETLEHAFELDSRWLMALHHVIEYHLA
                     RGETTQLRPLVEKVRHVNEARGAILECQIAISDRDYAQAARLARAAISAQPPTTELYG
                     CLMQSQILAGDFAGAEATGKEASERAPFDLEDFGNRTMAAELMLYRGQLSQYLSSLPD
                     TADRQRKITLAYWRTPTEFEELVQPAFGTRGQPIVPASQTLLAHMQGRDLQISYQKAF
                     EAELRAYGQGLAAELRGDLREAATELRHAMTAPAKGDIRMLAAHDLARVLVAQGDAAG
                     AKAACDEVIRPHLYMAYRAALLPDCVLWSGDVAQWRALADRWVGEFEQPSVIEIRRRL
                     AE"
     gene            complement(8873..9169)
                     /locus_tag="VMZ53_13510"
     CDS             complement(8873..9169)
                     /locus_tag="VMZ53_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29522.1"
                     /translation="MTATERITMVVLNEDGSRDKFTLSSWVGIPRKHELMWIGDDPYM
                     IIEVEYAVSEGFFGARSIDAAGVYVRRLTKDEQDAVARRLANPVRGKDEQPFRP"
     gene            complement(9166..9903)
                     /gene="dapB"
                     /locus_tag="VMZ53_13515"
     CDS             complement(9166..9903)
                     /gene="dapB"
                     /locus_tag="VMZ53_13515"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HUS29523.1"
                     /translation="MTKVCVLGPSGKMGRAVIEAAKAREDLQVTSTVDLGEDVAKGLA
                     AAQVYVDFTSPDGTRAAARAALANKTAAVIGTTGLRAGEEQAIAELAKVAPVVVAANF
                     SLGVNLLLGLVKQAAKALGTEWDAEVVETHHKAKRDAPSGTALAIARAIAAGHGVDYD
                     QVKRHTRDGDIGPRPRGEIGVATLRGGDVVGEHVAYFFGQAERIEIAHRATSRTIFAA
                     GSLRAATWVVGKPPGRYDMLDVLGFGS"
     gene            complement(9900..>10764)
                     /gene="dapA"
                     /locus_tag="VMZ53_13520"
     CDS             complement(9900..>10764)
                     /gene="dapA"
                     /locus_tag="VMZ53_13520"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HUS29524.1"
                     /translation="GCLTALVTPFANGKVDFDGLAKLVDWQIENGVDGIVSVGTTGES
                     ATLDVDEHVAVIAATVKAAKGRVPVVAGAGGNATSEALQLTKASEDAGADALLHVTPY
                     YNRPNQEGLYRHFEAIARSTKLPIILYNVPTRTACDLLTDTVVRLTAFDNIVGIKDAT
                     GNLVRGSELIAKVGDRLTVLSGDDGTSFPLYACGSRGVISVVSNVAPREMADMWDHVK
                     AGNWDKAKQIHYKLRVLNQLLFAEPSPAPTKAALSLLNRCSLECRLPLVQATGKLVEE
                     IRTEMRAQGLV"
BASE COUNT         1797 a         3582 c         3528 g         1857 t
ORIGIN      
        1 gcaaagttcg ggttcgaacc gagcacgcgc ggtgcggaat cgccttgcga ccacgcgagc
       61 aatcgcgtct gaccgccgga tgtctcgagc cagtacacgc catgcgcgtc ggcggtaatc
      121 gcggtgatca catcgccggc cgcgacgagc tgcgccgaac cgcccgggaa cttcggtacg
      181 cgcaacagga agtcctggct cgcggacgag tcgctcacga agtagaggtc ggtgccgctc
      241 atcgcgatcg cggtcgggcg cgtaccgtac gtgacgagct cgccggcgcc cggatcgctg
      301 aacacgccac tacagcccgc aaggctgatg aggagcatcc agagccgcat tcgcatgtcc
      361 ctataacccc gctcgccgcg cccgtgcgca aaatatttca gggggccttc ggcggccgtt
      421 tgtagacact cacgatgggc acaccgtcgg agcgcagcac gaacacgggc tgcacggtcc
      481 cgtacgactt ccagatcaaa tagtcgtgcc gattgaagtg cttctcgtgg atcacgagcg
      541 cgtattggct gcgctggatg ccgtcgtact cgtgcccggc gtcggggtag ccgcgcgaca
      601 gcatgccaag ccgcatgtac caaccccacg ctggcgacgc gtcgtgcgag tagaccgggc
      661 ccggcggcat ccactgcaaa aacggcaaca cgccctttgc cgagacgccc cagaattggc
      721 ggttcatgcc gcggtccgcg ccgccaggtg cgccgcctgc gagcgagttg taccaagtga
      781 gaccgtacgg ctgcgcatcg atcgtttcgg tcgccgccgc gaggatcatc gtccccgcga
      841 cgagcgatag ggcggccttg cgtgccgtcg cctcgccgat cgagcggaac gtctcgaggt
      901 agcgagctgc ttgccgcgcg gcccagatgc cgccgacacc ggcagcgata cacagcgtcg
      961 gcagcgcggg catccaatgc ttctcggcgc cgaagatcgg cgtggtacct aggaagaacg
     1021 ggcccatcga cgcggcagcg gaaagcccga gcaagagcgc cggtgctcgc tcgcgatctg
     1081 cgctcgtctt cgtgcgccag cggccgatcc acacgccgat gccggtcagc gcgccagcga
     1141 gagttccgat cgggaccgtg aacagcgtcg tcaccagcgc gacgtgccac ggaaaccgcg
     1201 gagcattcca gttgtggccg agatactcga agttgtagtg gacgtgcgtc aggtgaaacg
     1261 acagccactc cttcgtgtgc ttgatcggcg cgatccagag ccacggccac agatagaaca
     1321 gcacgaccgg cccgaggatc agcatcgaca cgatgacgcg ccagcgatgg aggaatgcgc
     1381 cgcgcaggcc gcccgcgacc gcgccgatga tcacgtagtg aagaccgagg gcgaacggca
     1441 acagaatcgc gttgtgcttc gtcgcgagag cgaggccgaa cgccgcgccg acttcgacgg
     1501 gaaaccgccg gccatcgagg cagcgccagt accagtagat cgtgagaaac cacatcgcca
     1561 tgatcggcgc gtcgaagcac gcgaggcctt cgtgaaacag cgcgcgcggc atcagcagca
     1621 tgcacgcagc tgcaagccct gcttcggcga agccccaaat cgcgagcgtc atcgagaaca
     1681 cgagccacac gacgagagcc gcgaatagcg cgcccggcaa ccgaaacgcg gtcagctcgt
     1741 cgaggatgtg cagcttgtcg tgaaacagct tgtgcgacgc accgatcagc gtcttcgcga
     1801 gcggcggatg ctcggcgttg ttgccgccgc cgatgccacc ccaatagtcg gtgatgccct
     1861 tcttcgagac gacgcccttg cccgtgaacg cgcggtccca ccactcggcg taacgcgagc
     1921 ccgcgttgaa gtagacgacc tcgtcgcgcg cgatgccaac ctcgcgctga ttcgcgagca
     1981 cgacgaagaa cgtcagcagc gcgagcccgt acccgacgag gcgttggcgt gtcactggtg
     2041 cgcctccgcc atgaagcaga cgagtcgctg cggcgcgttc gcgctgacga tgaacgtcac
     2101 gtcgctcggg ccaggcgccg tcgcggcagt gagcttgacc cacccatcgt cgacgcccgc
     2161 gctcgtcgca gcgacttgct ttccggcgat ctcgatcgcg agcgttgccg gggcgcgaat
     2221 gtctcggcgg gtgaagacat ccgcgaggcc gacaccgacg acgagcttcg accccagctg
     2281 cagctgcggg aacgtgatgc gcaccggatg attcggcgct ggcacgatct gcaagcactc
     2341 gtgcggcgcg aacccgactt cggcaagctc gagcgagggg cgcccgccgc cttcgacctt
     2401 catcgtcgcc atcgcgctct tcagatcgct cacgacgatc gccggctcgc gcgtatagag
     2461 attcaccgtc acgccggcga ccgtcgcggt cttcacgggc cgcagccccg cgacctccgg
     2521 tgcgtgctca ccctcgatcg acacttccca gatgcggccg aacttcgatg catccatccg
     2581 cgccgcgacg tcgatcggga tcagatcacc gaggtgcagg cgaccgaccg gatcgttcca
     2641 gctcggcgcg aacacgatga gatcaccggg ctgatacccc gcgcgcacca gcttctcggc
     2701 gcggtcccac gcatcgtcgc ccggaacgct cgcggcaagc cgaggcgtcg agcagacctc
     2761 ccacgtcgcg atgacgatca agaggagcgc cgggacaacc gcgacaggcg atggtctccg
     2821 agtcggcaac gcgtccgacg ctaccatgcg gtaatgtcgg gcggtgcggc tgtacgcgct
     2881 gctgttcgtg atcggcttgg tcgcctatgg cggccttgca gccaagcgcg tcttctcgcc
     2941 gtccgcggcg ccgcaattcg tcgtgcaggc cgactcgtgg ctccacggca agctctctgt
     3001 cacgccgacg aagaccgacg cagattggtc gaaggtcgag accgtcgagc tgcgagaccg
     3061 accgggcgag ctcgtgcagg ggcggcgcat gatcacgcgg ccgttctttc atccgctgcg
     3121 cggcgcagag atcccgaacg cgcagatcgg cggcacgcgc gaagtcactt ggtacatgtc
     3181 gttcccgccg ttcccgacgg tgatcatgct gccgtccgcc atcatctcgg gacgcgatgg
     3241 caacgatgtc ctgccgacga tcctgttcgc cgcgctgatc cttccgctct cgctgctcgt
     3301 gttgcgacgg ctcgccgctg cgcagctctc acagcgctca ctcggcgatg acctgtggct
     3361 cgtcgcgatg ctcgcgttcg gcagcgtgat gttcttctcc gcggtgcaag gaagcgtctg
     3421 gtacacggcg catgtcgtcg gtgtcgtgct cgcgctgatc tacgcgtggg gctcgatcga
     3481 ggcgaagcgg ccgatcatcg cgggtctcgc gctcggcgcc gcggctctca cgcgcacgcc
     3541 gatggcgttc atgttcccgc tgtttctcgc cgaggcgtgg cgcatggcgg gcggcgatcg
     3601 ccggacattc gtccgcggtg tcgtgccgtt cgcgattccg gtcgcggcgt tcgcgatcgc
     3661 gggcatggcg tacaacatcg ttcgctttca ctcgccgacc gagttcggtc acacgtacct
     3721 cgacgtccgc cagcaagcgc agatcgagca gtggggcctt gcgagttatc actacctgac
     3781 gcgcaacctc gccgtcgcgt tcacgctgct gccggagctg ccgggccact ggccgtggat
     3841 ccagatcagc ggtcacggcc tcgcgctctg gctgacgacg ccaatcttgc tgtacgcgct
     3901 gtggcccaca cagaagcccg ccattcatcg cgcgctgtgg atcaccgtcg cttgcgtcgc
     3961 gatcccgtcg ctgttctatc agaactcggg gtgggtgcag ttcggctatc ggttcagcct
     4021 ggactacatg gtgttcctga tgatgttgct cgcgatcggc ggccggcgct tcggctggct
     4081 cgcgcgaggc ctgatcatcg tcgggattct gatcaatctg ttcggcgcct acacgttcaa
     4141 tcgctcgggc cagtactacc actacaacta cgacgtcgtc gtcgcgcact gatacgatgg
     4201 tcgaatgaag ttggccgtgc tggtgtgcgc gcttgcggtg tcggcgtgca tccagtcggg
     4261 gctggttccg tgcggcgatc gactctgcgc gcgcgatgcg atgtgtatcg ccggcagcgt
     4321 ctgcgcgaat gccgatcaac tgagcgcgtg cgcaggcgcc gacgaaggca cggtgtgcac
     4381 gctcgtctcc ggaaccgtcg gtcgctgcac gctcggcgtc tgcgtgcctt cggggtgcgg
     4441 caacatgcgc atggatccgg gcgaggtctg cgacgacggc aacgtcaccg ccggcgacgg
     4501 ctgcagcggc acgtgcgact ccgacgagat gtgcggcaac ggcgtcctcg atgcgatcaa
     4561 aggtgagagt tgcgattgcg gcgccgacga cacgcgcgtg ccgcagagct gtacggggcc
     4621 gaactccgac gatccgagct cgccgtgtac gacgggttgc aggcttcggc gttgcggcga
     4681 cggcgaagtc gcgggccccg agcagtgcga cggaacgatg atgcccgacg gaatcgcgtg
     4741 cacgtcgtac ggcttctaca cgggaacggt tgcctgctcg gcgctgtgtc agttcgacat
     4801 cgcagggtgc agcgagcgtt gcggcgactc gatcgtgcag acggcctacg aggtctgtga
     4861 cggcacgccg cccgcggatc agacgtgtct cgacttcggc tatgacctcg gctggctcgg
     4921 ctgtacgggc gtctgcacgc caggtctcgc cgattgcgat cgcctcggat gggctcgtgc
     4981 acaccagcgc atcgcaactg caaccgacgt gattccccgc ggcaatcgat tgctcgtgcg
     5041 gtttttcgac ggcaaggtct cgctcctcat cgacgacgtc gcgaaggatc caacgggtac
     5101 gtttctcgcg accgcgtcga cggaaacgga ctcgtacgca atcgcgcaga cgtcggtcat
     5161 gcaccacgcg acggactggc aagcgctacc gatcgcgccg ccgtggacgg cccagccgac
     5221 ctacgcattc gcatcgtcgt cgctcggact gtttgtcgtc gtggagggcg acgccaatgc
     5281 gtatcgttgg aatggcacgc agtggcttac atacgcgatt ccgggtggag cgatcgccaa
     5341 gccggcgatc ggcgggtcac gcacgaggcc atacatctac gatccgagcg gcacctccat
     5401 cgcgatgtgg aacggcgcgg gcttcgctgc ggtgacgcca ccggcgaata ccacgacggt
     5461 gcatggcgtc gcagagacca gcacggccct ctacgttgcg accgaagacg gattgttcgg
     5521 gcgcgcgatc gtgtcgtcgg gcttcgggga tcaggggatc cctgctctgc agatcctcgc
     5581 caacagcaat gatggcgtcg acgtcctgac gacggccggc gtgatcctgc gactcggcaa
     5641 cggcgcgctg cagttctcgg caccgcccgg cacggcaggc atcagcgaag aaggcggccg
     5701 aatttttgtc acgggtgttc caagcggcgt gttcgcgatg ctgaagggca cgtggcaagc
     5761 gaacgatccg ccggccgcga cgccacagac gtacacgtac atccatggct cgggcccgct
     5821 gaaggacgac gtctacctcg tgacgggccc cgcgacgtgg gagctccaac cgggccgctg
     5881 ggtgaacatc acggtgcctc ccggaacggt cgacgcgatc cgcacggaag attcgctcgt
     5941 ctcgatcgat acatcgctca cgatctcgaa ggccactggc tcgacgacga agagcttctc
     6001 ggtgatcacc tcgccgaaga tgctgttcta cccgccgacg tcagggaagg tgttcatcgc
     6061 gggcgataag ggcgccgcca tcctgaacac gacatcgggc gtgctcgatg aagtgaatct
     6121 caacgcagat ctaaaagcga tccacggcgc gagcgcgacg aatgtgtttg cagttggtag
     6181 cgaccgcacc acgggcgtgg gcgtcgtgta tcggtacgac ggcatcaact ggaagccgat
     6241 cgacgtgccg ctcagtccgt ttccaccgct acgcgacgta ttcgtctctc cgacgggaaa
     6301 ggcctacgcc gtcacgcgaa acacggtgtt ttttggcagc gataagccct cggactggac
     6361 agcgctcgcg gtgccaggcg ccgacctcgt ctcgatcagc gggacgaagg acgacgactt
     6421 cttcgtcgca gggccgagcc agttcgggtt tccattcagc gggctcgccc actgggatgg
     6481 cactcacttc tatccggtgc gattgccggc taccgccgcg ccgcgcgacg tgtttgtttc
     6541 gatcggcgcc gtgtacgtgt tgctgaccga cgccacggca aaccgcgaca acgtctacgc
     6601 gctcgtccgc gcgacgcggt tctgattact cggcgagccg gcgccggatc tcgatgacgc
     6661 tcggctgctc gaattcaccg acccagcggt cggcaagcgc acgccactgc gcgacatcac
     6721 cgctccacag cacgcagtct ggaagcagcg ccgcgcgata cgccatgtag aggtgcgggc
     6781 gaatgacttc gtcgcacgct gctttcgcgc ccgcagcatc tccttgtgcg acgagcacgc
     6841 gcgcgagatc gtgtgccgca agcattcgga tgtcgccctt cgcgggtgcg gtcatcgcgt
     6901 ggcgcagctc ggtcgccgct tcgcgaagat cgccacgcag ctcggcggcg aggccttgtc
     6961 cgtacgcgcg cagctccgct tcgaatgcct tctgatacga gatctggaga tccctgccct
     7021 gcatgtgcgc gagtagcgtc tgcgacgccg gcacgatcgg ctgaccgcgt gtaccgaacg
     7081 ccggctgcac gagctcctcg aactccgtcg gcgtacgcca gtacgcgagc gtgatcttgc
     7141 gttggcggtc cgcggtatcg ggaagcgacg acaggtactg cgacagctgt ccgcgataga
     7201 gcatgagctc ggctgccatc gtgcgattgc cgaagtcctc cagatcgaac ggagcccgct
     7261 cgcttgcttc cttgcccgtc gcttcagcgc ccgcgaagtc accggcgagg atctgcgatt
     7321 gcatcagaca gccgtagagc tccgtcgtcg gcggctgtgc cgagatcgcc gctcgtgcga
     7381 gtcgcgccgc ctgcgcgtag tcgcggtcgc tgatcgcgat ctgacactcg aggatcgcgc
     7441 cgcgcgcctc gttcacgtgc cgcaccttct cgacgagcgg ccgcagctgc gtcgtctctc
     7501 cgcgcgcgag gtgatattcg atcacgtggt gcagcgccat gagccagcgc gaatcgagct
     7561 cgaacgcgtg ctcgagcgtc tcgacgccgc gctcgatctg ctggccatgc caacgcgctt
     7621 cgccgagggc gtactggatc tcgacggagc tcggatagcg atgctcggca tcctcgagat
     7681 atgcgatcgc ctgcgattcg cgacggctcg cgacgagctg tagtccctcg atcatgtcgc
     7741 ggtgaacggg gcgcaacttg tcgcgacctt cgaccgggtt cgatggaaag acgccgtgtg
     7801 tccaccacga cgtcaggagc atcatcatgc gcgcgtctgg atcgttcgga tgcgctctga
     7861 cgtactgctg gagcaggagc tcggcgcgac ggtgatcgcc gctctgcgtg agcgcaaaga
     7921 gctcgagtcg ccggggatcc tctgcgggct tctcgtgatg catgcgagtc cacgcgaccg
     7981 ccccgcagcc agcaccaagg gcaagcaatc cggccagcac agctgcggtc ttcctgcgcg
     8041 agcgcggtcg cttcgagtcg gtgaggacag cgagcgcttc gcgcgcagag ccgatgcgat
     8101 cctctggcgc agcgcgcagg cagcgcgaga tcagctcgcc gagcccgtac tcgggatcgc
     8161 acttcagccg cacgctcgcg atcgcggcgt cgctcggcgt ctcgaacgcg accatgcgct
     8221 ctcccgatac gagctcgtag agcgtggcag cgaggccgta tagatcggtg cgcgcgtcga
     8281 ccttcgcgcc gcgcagctgc tcgggcgcca tgtacgccgg cgtgccgagg ctctctccgg
     8341 tgcgcgtcac ttctgcgtcg accagccgcg cgacgccgaa atcgacgagc gtcaccttgc
     8401 cgcgcgcatc gacgataatg ttcgacggct tgatatcgcg atgaatgacg ccactcgagt
     8461 gagcggcatc gagcgctccg agcacctgct cggcaaaccc gcgcacacga acgggcgatg
     8521 acggaccgcg cgctgcgatc tcgcgcagcg actcgccgct aatgaggtcc atcacgagcc
     8581 agcccgcttc tgggttcgcg tcgtgaacgg cgacgatgtt tggatgcgcg agacgcgctg
     8641 cggcgcgagc ctcgcggata aaccgatcgc gcggctgcgt gtcgtcgccg gtgactgcaa
     8701 tgtgctcgat cactttgacg gcgacgtcgc gaccgagcgt cgtgtcatgc gcgcgataca
     8761 cgcggcccat gccgccggta ccgatcacct cgcgcagcac gtagcgctca gggagatccg
     8821 ctggacgatc gagatccgtt ccagcgcggc tcaccgcctc gatgataccg cctcagggac
     8881 ggaacggctg ttcgtctttg ccgcggaccg gattcgcgag acgccgtgca acggcatcct
     8941 gctcgtcctt cgtgagtcgt ctaacgtaga cgcctgctgc gtcgatgctg cgcgcgccga
     9001 agaaaccttc tgacaccgcg tactcgacct cgatgatcat gtacgggtcg tcgccgatcc
     9061 acatcagctc gtgcttgcgt gggatgccga cccacgagct cagcgtgaac ttgtcgcgcg
     9121 atccgtcctc gttgaggacg accatcgtga tgcgctcggt cgcggtcacg agccgaaccc
     9181 tagcacgtcg agcatgtcgt aacgacccgg cggtttgccg acgacccagg tcgccgcgcg
     9241 caacgagccc gccgcgaaaa tcgtccgcga ggtggcgcgg tgtgcgattt cgatgcgctc
     9301 cgcctgaccg aagaaatacg cgacgtgctc gccgacgacg tcaccgccgc gaagcgttgc
     9361 cacgccgatc tcgccgcggg ggcgagggcc gatgtcgcca tcgcgcgtgt ggcgtttcac
     9421 ctggtcgtag tcgacgccat gaccggcggc aatcgcgcgg gcgatcgcga gcgctgtccc
     9481 cgatggagcg tcgcgcttgg ccttgtgatg agtttcgacg acttccgcat cccactcggt
     9541 gccgagcgcc ttggcagcct gcttcacgag gcccagcaga agattcacgc cgagcgagaa
     9601 gttcgcggcg acgacgacgg gcgcgacttt cgccagctcg gcgatcgctt gctcttcgcc
     9661 ggcgcgaagg ccggtggtgc cgatcaccgc ggctgtcttg tttgcgagcg cggcgcgagc
     9721 ggctgcacgc gtgccatcgg gcgatgtgaa atcgacgtag acctgtgccg ctgcgaggcc
     9781 cttcgcgacg tcctcgccga gatcgaccgt cgacgtgact tgcagatcct cgcgcgcctt
     9841 cgccgcttcg atcacggcgc gacccatctt tccggacggg ccgagcacgc agaccttcgt
     9901 catacgaggc cctgagcgcg catctccgtc cgaatctctt cgacgagctt tccagtcgct
     9961 tgcacgagcg gcaggcggca ttcgagcgag cagcgattga ggagcgagag cgcggccttc
    10021 gtcggcgcgg ggctcggctc ggcgaacaag agctggttca ggacgcgcag cttgtagtgg
    10081 atctgcttcg ccttgtccca gttgccggcc ttcacgtgat cccacatgtc cgccatctcg
    10141 cgaggcgcga cgttggacac gaccgagatc acaccgcgcg agccacacgc gtagagcggg
    10201 aacgaggtgc cgtcgtcgcc gctgagcacc gtcaagcggt cgccgacctt cgcgatgagc
    10261 tcgctgccgc gcacgaggtt gccggtcgcg tctttgatgc cgacgatgtt gtcgaacgcg
    10321 gtgaggcgca cgacggtgtc ggtgagcaga tcgcacgcgg tgcgcgtcgg cacgttgtag
    10381 agaatgatcg gcagcttcgt cgagcgcgcg atcgcttcga agtggcgata gaggccttct
    10441 tgattcggcc ggttgtagta gggcgtgaca tgcagcagcg catccgcgcc ggcgtcctcg
    10501 gacgctttcg tcagctggag cgcttcgctc gtcgcgttgc cacccgcgcc tgcgaccacg
    10561 ggtacgcggc ccttcgccgc tttcacggtc gcggcgatga cagcgacatg ctcgtcgaca
    10621 tcgagcgtgg cggactcgcc ggtcgtaccg accgagacga tgccgtcgac gccgttctcg
    10681 atctgccagt cgaccagctt cgcgaggccg tcgaaatcga ccttgccgtt cgcgaatgga
    10741 gtgacgagcg cggtgagaca gcct
//
LOCUS       DATLBZ010000258        17345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6849020, whole genome shotgun sequence.
ACCESSION   DATLBZ010000258 DATLBZ010000000
VERSION     DATLBZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6849020"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /gene="murF"
                     /locus_tag="VMZ53_13525"
     CDS             <1..1695
                     /gene="murF"
                     /locus_tag="VMZ53_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HUS29525.1"
                     /translation="GAAVAELADLAVVTSDNPRTEDPRKILEAILPAVPKPFFVDVDR
                     RKAIRAAIAEATPGDVVVIAGKGHEDYQILGTTKIHFDDREEAAAAAAEREQRLIAGL
                     ARDASGELLGDGNGVCDRYTIDSRNVLPGSLYIAIRGENHDGHRFCLDAVKAGATAVM
                     VDREGRRWAPADKPELLAPELRALKHAAIVVDDTRIALGKLARAHRRAWEGEHRKLVA
                     ITGSAGKTTTKELTRAALAMSGTTHAAEGSLNNETGLPLTLLGLRLFHQFGVVEMGMR
                     GLGQIEYLTKIAEPDIAVVVNAGTAHIELLGSTDAIAQAKGEIFLGLRPGGTAIYPAG
                     DERLYRWAHTNAPGARFVTFGDGDGDVRIVEYAPTDAGGTAKLDVFGEIRELRLQLVG
                     KHAVLDACAALAAAHAAGASLDQAIAGLERAMPVAMRGEVVELGGRKVIVDCYNANPA
                     SMAAALRTLAERAKGGQKIAVLGDMLELGDHSVQAHREIGDLANKLGIGVIAIGTQAK
                     HIVDAAGGDAEHVASVDDAADRALARTEKAGGWILLKASRGMKLERVLEAMKESTP"
     gene            1695..2852
                     /gene="mraY"
                     /locus_tag="VMZ53_13530"
     CDS             1695..2852
                     /gene="mraY"
                     /locus_tag="VMZ53_13530"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HUS29526.1"
                     /translation="MLFHLLYGVLSKYVHVFQVFRYPSTRILAAAITSLLLSFLIGPW
                     FIERLKARQIGETIRDDGPQTHKKKAGTPTMGGSLILFCLAISTLLWCDLHSRLVWLA
                     ITVTISFGAIGFADDYEKVAKKNKKGISGKLRLFLEFAIAGGAMAYLFYSDTMSSDVR
                     LHVQLPFTNFYEQGLIIPAWAYVIFGAFVVVGTANAVNLTDGLDGLAIGPSIMNAGVF
                     LILAYIAGVPTTILSAQGPLSIAQYLHIAHINGSEELAIFAAALFGSGIGFLWYNSYP
                     AQVFMGDVGSLGIGSAIGMMAVLTKNELVLLIVGGLFVVEALSVIIQTSVFKATKRVE
                     GGKTIGKRVFLMAPIHHHYEKLGWDEPKIIVRFWLVSALLALLALGTLKMR"
     gene            2855..4243
                     /gene="murD"
                     /locus_tag="VMZ53_13535"
     CDS             2855..4243
                     /gene="murD"
                     /locus_tag="VMZ53_13535"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HUS29527.1"
                     /translation="MQLEGKKVVVVGLALTGVAVAKFCARRGARVIVTDGKPADKLSE
                     QMKLLGGVPVTLELGGHNESSFTSADLVVMSPGVPTLPEMTAARKAGVEVLAEIELAY
                     RFLDPGATLIAITGTNGKSTTTALTGKLCEHSGRPTFCGGNLGNMPLIDAVDHPANVE
                     GGWIVAEVAAFMLENCSTFAANAGVLTNVTEDHLDRFGTMERYAEMKGRIWDWQTPGD
                     VAIANAADPWVMKETAGIRSQLYTFDSRKGATAERGAFLDGNDIVLRLPDEERYAKDD
                     LVIVGNHNMENAMCAYLATRTLGLPPDAVRAGARAYRPLPHRMELVGDRGNIYYYDDS
                     KGTNVASVAASVRGFPRPLGLIAGGVDTGGSYEPMLEALDGVCKGIVLIGKAGPLIRD
                     AAAAHRVTYPVIDATDMHDAVRRATEMCSAGDAVVLSPACASYDMFQNYGHRGRVFRE
                     AVSAVGAKRLDQ"
     gene            4253..4642
                     /locus_tag="VMZ53_13540"
     CDS             4253..4642
                     /locus_tag="VMZ53_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29528.1"
                     /translation="MRALAIAVLLAACGGKPDPQPPKRPNNELIVGEFERRHQEAHTA
                     ARFKADGSITIASKADELDSKAIATGTYKLDGEKLTLVYTKGDLCEAGVEGTYNVVIS
                     KVGIRFTKLDDACERRAKIDGETWYRR"
     gene            4707..6020
                     /gene="ftsW"
                     /locus_tag="VMZ53_13545"
     CDS             4707..6020
                     /gene="ftsW"
                     /locus_tag="VMZ53_13545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HUS29529.1"
                     /translation="MAVLRRLVERVSSVRARLVGRLRRERSDDEPTKLPASASLIAIQ
                     HPSATLPERSYDLLLIGAVFALLTIGTIEIYSATAAEALTQWGSSTHFLTRQLLYITV
                     GAVAMWQAARFDYRRLRAWVYPLLLISLFLLAAVLGMPKINGAKRWIPLGPFTFQPVE
                     IAKVALVTYLAYSLGRKADQVKTFTIGFVPHLIVCGMMMVLLLAQPDLGSSVVLGLTT
                     LGMLFMAGARVSYILLAVLGAAPVAYHMVVGTPWRMQRFLAYFNPDAYANKEAYQFLQ
                     ARLAIGSGGLTGNGLGHGHQTLGYMPEAQNDFILAPIGEELGWIGIAVVLALFAILMW
                     RGIRAALGARDVFGGYLAFGITLMFGVQVLFNVGVVLGVVPNKGITLPLVSYGGSSLV
                     ITMFLVGLMLNVGRRAERTAKRASTERDPAARKKRVRVRVVSSAA"
     gene            6017..7093
                     /gene="murG"
                     /locus_tag="VMZ53_13550"
     CDS             6017..7093
                     /gene="murG"
                     /locus_tag="VMZ53_13550"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HUS29530.1"
                     /translation="MMRLLIAGGGTGGHLFPGVAIAEELRARDPDAVVKFVGTRRGIE
                     ARVLPDLGWDLEFIEVSGLKTVGAAGAVKGMFRLPKAMLQARRIVKTFKPDAVIGVGG
                     YASGPVVLAAKLRGIPTAICEQNSIPGLTNKLLGRVVRNVFLSFDESRRFFKAKKIVM
                     SGNPVRRDLVQKLLSAGASTDEKVHILVVGGSQGAVAVNELASIALSTLAKQLPLAIV
                     HQTGEKDLDATAARYKAAGVEADTRAFIKDMAAAYGRADLIIGRAGATTVAELAIAGK
                     PAIFIPYPFAADNHQELNAREMADKGAALMFKQSDLTADKLADALRPLVSDAAKRSEM
                     GARMKSLAKPGAAAAVIDWAQAKQ"
     gene            7137..8582
                     /gene="murC"
                     /locus_tag="VMZ53_13555"
     CDS             7137..8582
                     /gene="murC"
                     /locus_tag="VMZ53_13555"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HUS29531.1"
                     /translation="MFRKQDTKIHFVGIGGMGMCGIAEVLANMGYRVSGSDMNDTEIT
                     RHLSSIGCTVKQGHRADNLGESDVVVVSSAIKGYNPEVEAARARQIPIIPRAEMLNEL
                     MRMKYGIAVAGAHGKTTSTTMMHTVLMAAGYDPTAVIGGRVNSLGLANARWGKSDYLV
                     AEADESDGSFLTLTPTVAIVTNVDAEHLDHYGTYDNVKKAFTDFCNRVPFFGMSALCM
                     DNEGVQDILPNMTKRFTTFGISSQATYRARNIRPDGMSTRFVAWRRADELGEVTLPMP
                     GHHNVLNALGVLAVADFLGIPFDTYAKALGSFEGVARRFTVRGEVGGVTVVDDFGHHP
                     AEVRATLSGAKGSFGGRRVIAAFQPHRFTRTRDQWAEFPKAFHDADKVVICDIFAAGE
                     KPIDGITSEGLVRLAREAGHKDISYVPKREDLAKWLDQHAKAGDLVITLGAGNIQLTC
                     NDLIDLLEKRLGPATKKNLVRVQESSATVAR"
     gene            8579..8878
                     /locus_tag="VMZ53_13560"
     CDS             8579..8878
                     /locus_tag="VMZ53_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29532.1"
                     /translation="MNRLVVLALLVACKGDAPKPTMTPEQVISAVRDLADRGCECGTD
                     KECFRDVRTEWEQTRREILSNAKLLKDADLDAYNAERKRFGACGDAAGLAVFDNV"
     gene            8950..9711
                     /locus_tag="VMZ53_13565"
     CDS             8950..9711
                     /locus_tag="VMZ53_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29533.1"
                     /translation="MATSCEACGGPVNEAVVKCPHCGEKRASTAPAYTKAEIRAIIAN
                     EEALIAHQSSSTLFKALVLPHKHTRGLLRLVELALTAVTLPAVAAGAIGIWFLGARRS
                     KGAFSTSGEIAPVVVMTLLGGGAIWGWLGGQYAMMAVTAMWARAGLRMIAAGQAERAL
                     IDEDKRDAHRAASDPLIANKRERELIDEDAKRPSALAPSRVAVAPPRPSAPALPTARV
                     VSTAEPVKPSVPAIAVKTPEPVTPTDPNGEPSILR"
     gene            9745..10479
                     /locus_tag="VMZ53_13570"
     CDS             9745..10479
                     /locus_tag="VMZ53_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29534.1"
                     /translation="MRFTIALLVAGCSASHAPKSSVSHRAAPAADGTLAPVLETPAGS
                     DDCPLRWDELARATPPIVEMVQSATPRKAMAADTNCPGFDPTRMTCPDGVDCETGRVR
                     GGHQAVVTRVSTSSASFVTIGFAVEDKGFACIAMSMPLMRSIYPFAPRMLDEPLLSDL
                     DGDGESELLVWSRLPWGDAEEDSAFVPVVYAFDGSRLVRRDDFATKLRSKVATVYRDV
                     VDRGKAAKNAACFTSVATAFSPPPME"
     gene            10508..11281
                     /locus_tag="VMZ53_13575"
     CDS             10508..11281
                     /locus_tag="VMZ53_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29535.1"
                     /translation="MRLLFAVMLLGCSSTPSADPAARQGPPDPAKPAPIANRTPPDAA
                     VAGDAPLAKLDPILIEPASGRSCALRWDELRGAAPPTIDLVPASSKLTDDCPEGFDPV
                     SNICAEGAGCVHATMRGGEEATLSIRGPEGSGRFNAVGLSIGGKRFACITASTVGWRL
                     IAESGDKLAPLKWLEDVDGDKDAELVIWTRLPWGDSEVANALMPVVYVLDGDRLVRRD
                     DRAKALRVKVAGVYHGLVNLAPDGEKKACFVALTAALGS"
     gene            11305..12333
                     /locus_tag="VMZ53_13580"
     CDS             11305..12333
                     /locus_tag="VMZ53_13580"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HUS29536.1"
                     /translation="MPIADVKRDVPLAPLTTLGIGGLAKRFARVTSEGELADALGDAD
                     SANERVLILGGGSNLVVLDEGWSGLVLQMAIEGVTIESDGDGFARVHAAAGVVWDDFV
                     DEMVRNGLGGVECLSGIPGLVGATPMQNVGAYGQEVADTIVRVHAFDREAFEPVAFAP
                     AACEFAYRTSAFKGKDRYVITEVEFRLPKRVDSMPIKYAELAKALGLQEGATASLASV
                     RDTVIGLRRKKGMVVDAADPDSRSAGSFFTNPIVAAEVAATLTDAPMWPQPDGRVKLS
                     AGWLIERAGFTKGTVRGNVGISSKHALALVNRGGATAAELLAFAREIQEAVRSKLGVE
                     LQMEPVIV"
     gene            12330..13265
                     /locus_tag="VMZ53_13585"
     CDS             12330..13265
                     /locus_tag="VMZ53_13585"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HUS29537.1"
                     /translation="MKHPFAGKRVAVVMGGLSAEREVSLNTGAGVLAALQEKSWDAVG
                     IDWKEGTSLPSLLAASGAHVVWNALHGTYGEDGAVQGLCGCMLLPCTGSGILASALAM
                     DKVMSKRIFESNGVPTPRWKLLPHDGPADGSDAEALLADFPLPCVVKPANEGSSVGVS
                     IVEERGQLAAAVATARAFHGQVIVEDYIAGTEVFVGILDGRVLGSVEVRPATKFYDYE
                     AKYKRNDTQYLIPPQLPADVIARTEQLALAAYNALGCSGHSRPDLRITTAGEPYVLEV
                     NTLPGMTKTSLLPKIAKSIGMDYGTLCEQILASAK"
     gene            complement(13324..14079)
                     /locus_tag="VMZ53_13590"
     CDS             complement(13324..14079)
                     /locus_tag="VMZ53_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29538.1"
                     /translation="MKKISIAMFSMIAMAGVASAQTAKGSASAGATVNAGGATGAAKG
                     TAGAATGTATGAAKSGAGAATGAAKTGADAAAGAAKGGATATVQAGAAMEMPKPPAEI
                     KDTLKAAGARATCKGTGLGMDMKSEVKFAGTVTRKVDLDGWWIHESMTGTMGEGKAKG
                     TMKMENYMTFDSKLGKWRAVAVMNDGTQMVGTADFKDGKLEAVWDTKGGMGDGMFKDH
                     GDMTDKKVGMHMWGEMSMDKGKTWTKVYDMNCK"
     gene            14131..15477
                     /locus_tag="VMZ53_13595"
     CDS             14131..15477
                     /locus_tag="VMZ53_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29539.1"
                     /translation="MIVGLIDDVIGSRFTLSLFVSVAYLAACGGTPRETAKPVVAKPH
                     TAAPHHLDDPDLNPPPPKKLLSIEWKSVTLTSEADALALWKTIAPTGDDYTEKLDEIP
                     DDGPIATQLALALLHGGNFSCSPTPSQCGAPVDVPEPMPTATFDDPCLRRILAMWSLQ
                     QLDDDDVQWVRDSLKTIVAIPPPESQLVMAALDVVPTSEQDLKLELLSIAQSAGHREL
                     ANGLVGSLDEAHLVDAAQKHHMSGPLDMLSAEAHRAVFIAAINDAKLTTNARLQAITE
                     IVTSEDKLSKETRATLATATRSPDCTVAAAAARALVQHGEKKFAPARPANRSSAAMMR
                     AMCVLASYEQLQGNDEASYLLGYVPAKGLEVVTVTYDQYSDADDDGDGDPHTQRTTNI
                     VPRTEVVLPELEDLVRAMAHCTGTVCRSDDREFRFTFKGDQLRRVEVVERPPCVPR"
     gene            15483..15782
                     /locus_tag="VMZ53_13600"
     CDS             15483..15782
                     /locus_tag="VMZ53_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29540.1"
                     /translation="MSRWLGLVLVLGLTATPAYAQVFKPKTGKTAKAKSDKAEKAEKT
                     EKAEPAEKKAKSPGKKRVTTKSKKSSSRDSDQASSSGPKDSDKDFVKITDDDDVE"
     gene            16054..16815
                     /locus_tag="VMZ53_13605"
     CDS             16054..16815
                     /locus_tag="VMZ53_13605"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HUS29541.1"
                     /translation="MLRRSVPALIGLAVLGTVGGTAWAGYRFVTHSPRFAVTTIDVTG
                     NRQVTTDEIMGSLPAHAGDNVFSVDLDAVSRAVKTNPWIAQAEAHRVLPHTIAISVRE
                     HVAAAVVELSGLYLADEQGHPFKKADLTIDEGAGLPIITGLDRAQYGANPDAGAAQVR
                     SALDALARWNENGDRPSIGELHLDPHGNLTLVTYEHAIKIQLGVPNDFLAMRLATFDA
                     TWNELSSSERSRMRAIHLDAHSDQVTVAFAPEKDQ"
     gene            16822..>17345
                     /gene="ftsA"
                     /locus_tag="VMZ53_13610"
     CDS             16822..>17345
                     /gene="ftsA"
                     /locus_tag="VMZ53_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HUS29542.1"
                     /translation="MAKPKSNEIIVGLDIGTTKIACIVGEVTEEGVDIIGIGTSASKG
                     LRRGCVVNIDATVASIQQAVDEAENMAGCEISSVYAAISGAHVKGLNSHGIIAVKDKE
                     VRESDIARVIEQAKAVAIPMDREVLHVLPQQYVVDDQDGIRDPLGMAGVRLEAKVHIV
                     TTAVTSAQNVVKCA"
BASE COUNT         3036 a         5801 c         5608 g         2900 t
ORIGIN      
        1 ggcgctgcgg tcgcggagct cgccgacctc gccgtcgtga cgagcgacaa cccgcgcacc
       61 gaagatccgc gcaagatcct cgaggcgatc ctgcccgctg tcccgaaacc gttcttcgtc
      121 gatgtcgatc ggcgcaaggc catccgcgct gcaatcgccg aggccacgcc gggcgacgtc
      181 gtcgtgatcg ccggcaaggg ccacgaggac taccagatcc ttggcacgac gaagatccac
      241 ttcgacgatc gcgaagaagc cgcagctgca gcggccgaac gcgagcagcg gttgatcgca
      301 ggccttgctc gcgacgcgag tggcgagttg ctcggcgacg gcaacggcgt ctgcgatcgc
      361 tacacgatcg atagccggaa cgtgctcccg ggttcgctat acatcgcgat ccgtggcgag
      421 aaccacgacg gccatcgctt ctgcctcgat gccgtcaaag ccggcgcgac agccgtcatg
      481 gtcgatcgcg agggtcgtcg ctgggctccc gccgacaagc cggagctgct cgcacccgag
      541 ctccgcgcgc tgaaacacgc cgcgatcgtc gtcgacgaca cccgcatcgc gctcggcaag
      601 ctcgcccgcg cacaccgccg cgcgtgggaa ggcgaacacc gcaagctcgt cgcgatcacc
      661 ggttccgccg gcaagacgac gaccaaagag ctgacgcgcg ccgcgctcgc gatgtcgggc
      721 acgacgcacg cggccgaagg ctcgctcaac aacgagaccg gcctgccgct cacgctcctc
      781 ggcttgcgcc tgttccatca gttcggcgtc gtcgagatgg gcatgcgcgg cctcgggcag
      841 atcgaatatc tgacgaagat cgcggagccc gatatcgccg tcgtcgtcaa cgccggcacc
      901 gcgcacatcg agctgctcgg ctcgacggat gcgatcgccc aagcaaaggg cgagatcttc
      961 ctcggcctgc gtcccggcgg caccgcgatc tatcccgcgg gcgacgagcg cctctatcgg
     1021 tgggcacaca cgaatgcccc cggtgctcgc ttcgtcacat tcggcgacgg cgatggcgac
     1081 gtgcggatcg tcgagtacgc gccgaccgac gccggtggca cggcaaagct cgacgtgttc
     1141 ggcgaaatac gcgagcttcg gctccagctc gtcggcaagc acgcggtgct cgatgcgtgc
     1201 gccgctctcg ctgctgcaca tgctgccggc gcgtctctcg atcaagcgat cgccggtctc
     1261 gagcgtgcga tgccggtcgc gatgcgcggc gaagtcgtcg agctcggtgg ccgcaaggtg
     1321 atcgtcgatt gctacaacgc gaatcccgcg tcgatggccg ctgcactgcg cacgctcgcg
     1381 gagcgcgcca aaggtggcca gaagatcgcg gtgctcggtg acatgctcga gctcggcgac
     1441 cacagcgtgc aagcccatcg cgagatcggc gacctcgcga acaagctcgg catcggcgtg
     1501 atcgcgatcg gcacgcaggc caagcacatc gtcgatgccg ccggcggcga tgccgagcat
     1561 gtcgcatccg tcgacgacgc cgccgatcgt gcgctcgctc gcaccgagaa ggcaggcggc
     1621 tggatcctgc tcaaggcatc gcgcggcatg aagctcgagc gcgtcctcga ggcgatgaaa
     1681 gagagtacgc cctagtgctg tttcatctgc tgtacggcgt cctctcgaaa tacgtccacg
     1741 tatttcaggt ctttcgttat ccgtcgacgc gcatcctcgc ggccgcgatc acgtcgctgc
     1801 tgctctcgtt cttgatcggt ccgtggttca tcgagcgact caaggcgcgg cagatcggcg
     1861 agacgatccg cgacgacggc ccgcagacgc acaagaagaa ggccggcacg ccgacgatgg
     1921 gcggctcgct gatcctgttc tgcctcgcga tctcgacgct gctctggtgc gacctccata
     1981 gccgcctcgt gtggctcgcg atcaccgtca cgatctcgtt cggcgcgatc ggcttcgcgg
     2041 acgattacga gaaggtcgcg aagaagaaca agaagggcat tagcggcaag ctgcgcttgt
     2101 tcctcgagtt cgcgatcgcc ggtggcgcga tggcctacct cttctacagc gacacgatga
     2161 gcagcgacgt gcgcctccac gtccagctgc cattcacgaa cttctacgag cagggcctca
     2221 tcattcctgc gtgggcgtac gtgatcttcg gcgcgttcgt cgtcgtcggc accgcgaacg
     2281 ccgtcaacct caccgacggt ctcgacggtc tcgcgatcgg tccgtcgatc atgaacgccg
     2341 gcgtgttcct gatcctcgcg tacatcgcgg gcgtcccgac gacgatcctg tccgcacaag
     2401 gtccgctctc gatcgcgcag tacctccaca tcgcgcacat caacggctcc gaagagctcg
     2461 cgatcttcgc cgccgcattg ttcggaagtg gcatcggctt cctttggtac aactcgtatc
     2521 ccgcgcaggt attcatgggc gatgtcggct cgctcggcat cggcagcgcg atcggcatga
     2581 tggcagtgct gacgaagaac gagctcgtgc tgctcatcgt cggcggtctg ttcgtcgtcg
     2641 aggcgctgtc cgtgatcatc cagacgtcgg tgttcaaggc gacgaagcgc gtcgagggcg
     2701 gcaagacgat cggcaagcgc gtgttcttga tggcaccgat ccatcaccat tatgaaaagc
     2761 tcggctggga cgagccgaag atcatcgttc ggttctggct cgtctccgcg ctgctcgcgc
     2821 tgctcgcgct gggcacgctg aagatgcgat agctatgcaa ctggaaggca agaaggtcgt
     2881 cgtggtcggc ctcgcgctga ccggcgtcgc agtcgccaag ttctgcgcgc gccgcggcgc
     2941 tcgcgtgatc gtcaccgacg gcaaaccagc cgacaagctc tccgagcaga tgaagctcct
     3001 aggcggcgtg cccgtcacgt tggagctcgg cggtcacaac gagtcgtcgt tcacgtcggc
     3061 cgatctcgtc gtgatgtcgc ccggcgtgcc gacactcccc gagatgaccg ccgcacgcaa
     3121 agccggcgtc gaagtcctcg ccgagatcga gctcgcgtat cgcttcctag atccgggcgc
     3181 gacgctcatc gcgatcacgg gcacgaacgg caagtcgacg acgaccgcgc tcaccggcaa
     3241 gctgtgcgag cactccgggc gtccgacgtt ctgcggcggc aacctcggca acatgccgct
     3301 catcgatgcc gtcgatcatc ctgcgaacgt cgagggtggc tggatcgtcg cggaggtcgc
     3361 cgcattcatg ctcgagaact gctcgacgtt tgcggctaac gccggcgtgc tgacgaacgt
     3421 caccgaagat cacctcgatc gcttcggcac gatggagcgc tacgccgaga tgaaggggcg
     3481 catctgggac tggcagacgc ccggcgatgt cgcgatcgcc aatgccgctg atccatgggt
     3541 catgaaggag acggcgggca ttcgcagcca gctctacacg ttcgattcgc gcaagggcgc
     3601 cacggccgag cgcggcgcat tcctcgacgg taatgacatc gtgcttcgcc taccagacga
     3661 agagcgctac gcgaaagacg acctcgtgat cgtcggcaat cacaacatgg agaatgcgat
     3721 gtgcgcctat ctcgcgacgc gcacgctcgg tctcccgccc gatgccgttc gcgccggtgc
     3781 tcgcgcgtat cgcccgttgc cgcaccgcat ggagctcgtc ggcgatcgcg gcaacatcta
     3841 ctactacgac gacagcaagg gcacgaacgt cgcgtcggtc gcggccagcg tgcgcggctt
     3901 ccctcgccca ctcgggctca tcgccggtgg cgtcgacacg ggcggctcgt acgagcccat
     3961 gctcgaagcg ctcgacggtg tgtgcaaagg catcgtgctc atcggcaagg cgggcccgct
     4021 cattcgcgac gccgccgccg cgcatcgcgt cacgtacccg gtgatcgacg cgaccgacat
     4081 gcacgacgcc gttcggcgcg cgacggagat gtgcagtgcc ggcgacgccg tggtgctctc
     4141 gcctgcctgc gcgagctacg acatgttcca gaactacggc catcgggggc gcgtgttccg
     4201 cgaagcggtt tccgccgttg gcgccaaacg cctcgatcag taagatcgag acatgcgcgc
     4261 actcgcgatc gctgtcctgc ttgcagcatg tggtggcaag cccgatccac agccaccgaa
     4321 gcggccgaac aacgagctca tcgtcggcga gttcgagcgc cggcatcagg aggcgcatac
     4381 cgcggcgcgg ttcaaggcgg atggctccat cacgatcgcg tcgaaggccg acgagctcga
     4441 cagcaaggcg atcgcgaccg gcacctacaa gctcgacggc gagaagctga ccctcgtcta
     4501 tacgaagggc gacctctgcg aggcgggcgt cgagggcacc tataacgtcg tcatctcgaa
     4561 ggtcggcatc cgatttacga agctcgacga cgcgtgcgag cggcgcgcga agatcgacgg
     4621 cgagacctgg taccggcgct agccaaccta cctacatcac gctgaaaagt ggcgccgtgg
     4681 acacgccggt ccgaggatga ggggccatgg cagtgctccg tcgcctcgtc gaacgtgtgt
     4741 cgtccgttcg ggctcgtctc gtcggcagac tccgacgcga gcgcagtgac gacgaaccga
     4801 cgaagcttcc ggcgagcgca tcgctcatcg cgatccagca tccgagcgcg acgttgcccg
     4861 aacggagcta cgaccttctg ctcatcggcg cggtgttcgc gctgctcacg atcggcacca
     4921 tcgagatcta ctcggcgacc gcggccgaag cgctgacgca gtggggaagc tcgacgcact
     4981 ttctgacgcg ccagctgctc tacatcaccg tcggtgcggt ggcgatgtgg caagcggcgc
     5041 gcttcgacta ccgccggctg cgcgcatggg tgtatccgct gctgctgatc tcgctgttcc
     5101 tgctcgcggc ggtgctcggc atgccgaaga tcaacggtgc gaagcgctgg attccgctcg
     5161 ggccgttcac gtttcagccc gtcgagatcg cgaaggtcgc gctcgtgacg tatctggcgt
     5221 actcgctcgg ccgtaaagcg gatcaggtga agacgttcac gatcggcttc gttccgcact
     5281 tgatcgtgtg cggcatgatg atggtgctcc tcctcgcgca accggacctc ggctcgtcgg
     5341 tggtgctcgg cctgacgacg ctcggcatgc tgttcatggc cggcgcgcgc gtctcgtaca
     5401 tcttactcgc cgtcctcggt gcggcgcccg tcgcgtatca catggtcgtc ggcacgccgt
     5461 ggcgcatgca gcgcttcctc gcgtacttca atcccgacgc atatgcgaac aaggaggcgt
     5521 accagttcct ccaagcgcgt ctcgcgatcg gctccggcgg gctcaccggc aatggcctcg
     5581 gccacggtca ccaaacgctc ggctatatgc cggaggcgca gaacgacttc atcctcgcgc
     5641 cgatcggcga ggagctgggt tggatcggca tcgccgtcgt gctcgcgctc tttgcgatcc
     5701 tcatgtggcg aggcattcgc gcggcactcg gcgcacgcga cgtgttcggt ggctacctcg
     5761 cgttcggcat cacgctgatg ttcggcgtgc aggtgctgtt caacgtgggc gtcgtgctcg
     5821 gcgtcgtgcc gaacaagggc atcacgctgc cgctcgtcag ctacggtggc agctcgctcg
     5881 tgatcacgat gttcctcgtc gggctgatgc tcaacgtcgg ccgccgcgcc gagcgcaccg
     5941 ccaagcgcgc gagcacagag cgagatccag ccgcgcgaaa gaaacgcgtg cgcgtccgcg
     6001 ttgtgtcgtc tgcagcatga tgcgcctact cattgccggc ggcggaaccg gtggccactt
     6061 gtttcccggc gtcgcgatcg ccgaggagct gcgcgcgcgt gatccagacg cggtcgtgaa
     6121 gttcgtcggt acgcgtcgag gaatcgaagc tcgcgtgctg ccggatctcg gttgggacct
     6181 cgagttcatc gaagtgagcg ggctcaagac cgtcggtgcg gccggcgccg tgaagggcat
     6241 gttcaggctc ccgaaggcga tgctccaggc gcgccggatc gtgaagacgt tcaagcccga
     6301 tgcggtgatc ggcgtcggtg gatacgcgtc gggcccggtc gtcctcgccg cgaagctgcg
     6361 cggaattcct acggcgatct gcgagcagaa ctccatcccg ggcctgacga acaagctgct
     6421 cggccgcgtc gttcgcaacg tgttcctgtc gttcgacgag agcaggcgct tcttcaaggc
     6481 caagaagatc gtgatgagcg gcaatcccgt gcgtcgcgat ctcgtgcaga agctgctgtc
     6541 cgcgggcgcg agcaccgacg agaaggtgca catcctcgtc gttggtggct cgcaaggcgc
     6601 cgtcgcggtg aacgagctcg catcgatcgc gctctcgacg ctcgcgaagc agctgccgct
     6661 cgcgatcgtg caccagactg gcgagaagga tctcgatgcg acggctgctc gctacaaggc
     6721 cgctggcgtc gaagcggaca cgcgcgcgtt catcaaggac atggccgccg cgtacgggcg
     6781 cgcggatctc atcatcggcc gcgccggtgc gacgacggtg gccgagctcg cgatcgccgg
     6841 taagcccgcc atcttcattc cctacccgtt cgccgcggac aatcaccaag agctcaacgc
     6901 gcgcgagatg gccgacaagg gcgcagcact gatgttcaag cagtccgacc tcacggcgga
     6961 caagctcgcc gatgcccttc ggccgctcgt ctccgacgct gcgaagcgct ccgagatggg
     7021 tgcgcgcatg aaatcgctcg caaaacccgg tgccgctgcc gcggtcatcg attgggccca
     7081 agcgaagcaa tgatgcgtgg tatttaggcg ttcgatctac ggaaggtcgt tcgcagatgt
     7141 ttcgcaagca agacaccaag atccacttcg tcggcatcgg cggcatgggc atgtgcggca
     7201 tcgccgaggt gctcgccaac atgggctacc gcgtgtcggg ctccgacatg aacgacaccg
     7261 agatcacgcg ccacctctcg tcgatcggct gcaccgtcaa gcaaggccat cgcgcggaca
     7321 acctcggcga gtccgatgtc gtcgtcgtgt cgagcgcgat caagggctac aaccccgagg
     7381 tcgaggccgc acgcgcgcgc cagattccga tcatcccgcg cgccgaaatg ctcaacgagc
     7441 tgatgcgcat gaagtacggc atcgccgtcg cgggcgctca cggcaagacc acgtcgacga
     7501 cgatgatgca caccgtgctc atggccgccg gctacgaccc gaccgccgtc atcggtggcc
     7561 gcgtcaactc gctgggtctc gcgaacgcgc gctggggcaa gagcgattac ctcgtcgccg
     7621 aagccgacga atccgacggc tcgttcctca cactgacgcc caccgtcgcg atcgtgacga
     7681 acgtcgacgc cgagcatctc gatcactacg gcacgtacga caacgtgaag aaggcgttca
     7741 ccgacttctg caatcgcgtg ccgttcttcg gcatgtcggc gctgtgcatg gacaacgaag
     7801 gcgtccaaga catcctgccg aatatgacga agcgcttcac gacattcggc atctcgtcgc
     7861 aggccaccta ccgtgcgcgc aacattcgtc ccgatggcat gagcacgcgc ttcgtcgcgt
     7921 ggcgccgcgc cgacgagctc ggcgaagtca cgctgccgat gcccggccat cacaacgtgc
     7981 tcaacgcgct cggcgtcctc gccgtcgccg acttcctcgg cattccgttc gacacctacg
     8041 ccaaagcgct cggctccttc gaaggcgtcg cccgtcgctt cacggtgcgt ggcgaagtgg
     8101 gcggtgtcac cgtcgtcgac gatttcggtc atcacccggc cgaagtccgc gccacgctct
     8161 ccggcgcgaa gggcagcttc ggcggccgtc gcgtcatcgc cgcgttccag ccgcaccggt
     8221 tcacgcgcac gcgcgatcag tgggcagagt tcccgaaggc attccacgac gccgacaagg
     8281 tggtcatctg cgacatcttc gccgcaggcg agaagcccat cgacggcatt acgagcgaag
     8341 gcctggtgcg gctcgcgcgc gaagcgggtc acaaagacat ctcgtacgtg ccgaagcgcg
     8401 aggacctcgc gaagtggctc gaccagcacg cgaaggcggg cgacctcgtc atcacgctcg
     8461 gcgccgggaa catccagctg acgtgcaacg acttgatcga tctcctcgag aagcgcctcg
     8521 gcccggcgac gaagaagaat ctcgtgcgcg tgcaggaatc gagcgcgacg gtcgcgcgat
     8581 gaatcgcctc gtcgtgctcg cgctgctggt tgcctgcaaa ggcgatgcac cgaagccgac
     8641 aatgacgccc gagcaagtga tcagcgcggt tcgcgatctc gccgatcgag gctgcgagtg
     8701 cggcacggac aaggaatgct ttcgcgacgt gcgcaccgag tgggagcaga cgcgccgcga
     8761 gatcctgtcg aacgcgaagc tgctgaagga cgccgacctc gacgcataca acgccgagcg
     8821 caagcgcttc ggtgcgtgcg gcgacgcggc cggcctcgcc gttttcgaca acgtgtaatc
     8881 agcgctacgc caattcggca acggccggtt tttcgccgcg cagacgccaa gctctggtag
     8941 atcttcgtcg tggcgacgag ctgtgaagcc tgcggcggac cggtcaacga agcggtcgtg
     9001 aaatgcccgc actgcggcga gaagcgcgcg tcgaccgcgc cggcgtacac gaaggccgag
     9061 attcgcgcga tcatcgcgaa cgaagaggcg ctcatcgctc accagagctc gtcgacgctc
     9121 ttcaaggcgc tcgtgctacc ccacaagcac acgcgcggcc tgctccgtct cgtcgagctc
     9181 gcactcacgg ccgttacgct gcctgcggtc gcagcgggcg cgattggcat ttggttcctc
     9241 ggcgcgcgtc gcagcaaggg cgcgttctcg acgagcggtg aaattgcgcc cgtcgtcgtc
     9301 atgacgctgc tcggcggcgg cgcgatctgg ggctggctcg gcgggcagta cgcgatgatg
     9361 gccgtcactg cgatgtgggc tcgcgccggc ctacgcatga tcgcggcggg ccaggcagag
     9421 cgcgccctga tcgacgaaga caagcgcgac gcgcaccgcg ctgcgtccga cccgctcatc
     9481 gcgaacaagc gcgaacgcga gctgatcgac gaggacgcca aacggccgag cgcactggct
     9541 ccctctcgcg tggccgtcgc tccgccgcgc ccgtccgcgc ccgcattgcc aaccgcacgc
     9601 gtcgtctcga cggccgagcc cgtgaagcct tcggtgcccg cgattgccgt gaagacaccc
     9661 gagccggtca ctccgaccga tccgaacggc gagccgagta tcctgcgcta aggaatgcgc
     9721 ctgcccgcat cacgtcgctc gctcatgcgg ttcaccattg cgcttctcgt cgcgggctgt
     9781 tcggcttcgc acgccccgaa gtcgtcggtg tctcatcgcg ccgcgcctgc cgcggacggg
     9841 acgctggcgc cggtcctcga gacgcccgca ggaagtgacg actgcccgct ccggtgggac
     9901 gagctcgctc gcgcaactcc accgatcgtc gagatggtgc agtcggcaac tccccgcaag
     9961 gcgatggccg cagacacaaa ctgtcccggg ttcgatccga cccgcatgac gtgcccggat
    10021 ggagtcgatt gcgagaccgg acgcgtccgg ggcggtcacc aggcggtcgt gacgcgcgtg
    10081 tcgacgagct cggcgagctt cgtcacgatc ggtttcgccg tcgaagacaa gggatttgcg
    10141 tgtatcgcga tgtccatgcc gctcatgcga tccatctatc cgttcgcgcc tcgcatgctc
    10201 gacgagccat tgctctctga cctcgacgga gacggcgaga gcgagctcct ggtttggtct
    10261 cggttgccat ggggagacgc ggaggaggac agcgcgttcg tgccggtggt ctacgcgttc
    10321 gacggctcac gcctggtccg tcgcgacgac ttcgcgacga agctgcgcag caaggtcgcg
    10381 accgtgtatc gagacgtcgt ggaccgcgga aaagccgcaa agaacgcagc atgcttcaca
    10441 tccgtcgcga ccgcgttctc gccaccgccg atggaatgag cgcatcgcac aaggcgttac
    10501 aatgatcgtg cgcctcctgt tcgcggtcat gcttctgggt tgctcgagta ccccgtcggc
    10561 cgatcccgct gcacgtcagg gaccgcccga ccccgcgaag cctgcaccga tcgcgaatcg
    10621 cacgccgccg gatgcagcgg tggcaggcga tgcgccgctc gcgaagctcg acccgatact
    10681 gatcgagccc gcgagtgggc ggtcgtgcgc gctccgttgg gacgagctcc gcggtgcggc
    10741 gccgccgacg atcgatctcg tgcccgcgag cagcaagctc accgacgact gccccgaagg
    10801 attcgatccg gtgagcaaca tctgtgcgga aggcgccggc tgtgttcacg cgacgatgcg
    10861 cggcggcgag gaagcgacgc tctcgattcg cgggcccgag ggctccggtc gcttcaacgc
    10921 ggtcggtctg tcgatcggcg gcaagcgctt cgcgtgcatc accgcgtcga cggtcggttg
    10981 gcggctcatc gcggagtccg gcgacaagct cgcgccgctg aagtggctcg aggatgtcga
    11041 tggcgacaag gatgccgagc tcgtgatctg gacgcggctg ccgtggggcg actcggaggt
    11101 cgcgaacgcg ctgatgccgg tcgtatatgt cctcgacggt gaccggctcg tgcgacgcga
    11161 tgatcgtgcg aaggcactgc gcgtgaaagt cgcgggcgtg tatcacgggc tcgtcaatct
    11221 cgcgcccgat ggagagaaga aggcctgctt cgttgcgttg acggccgcgc tcgggagctg
    11281 acggtggtga tatcgtcgcg tcgcatgccg atcgcggatg tgaagcgcga tgtgccgttg
    11341 gcaccgttga cgacgctcgg catcggcggc ttggcgaagc ggtttgctcg cgtgacgtcg
    11401 gaaggcgagc ttgcggacgc gcttggcgac gcagactccg cgaacgagcg cgtgctgatc
    11461 ctcggcggtg gctcgaatct cgtcgtgctc gacgagggct ggtccgggct cgtcctgcag
    11521 atggcgatcg agggcgttac gatcgagagc gacggcgacg gctttgcgcg cgtgcacgcg
    11581 gcggcaggcg tggtgtggga cgacttcgtc gacgagatgg ttcgcaacgg gctcggcggt
    11641 gtcgagtgct tgtcgggaat tcccgggctc gtcggcgcaa cgccgatgca gaacgtcgga
    11701 gcgtatgggc aggaggtcgc cgatacgatc gtgcgcgtgc acgcattcga tcgcgaagcc
    11761 ttcgagcccg tcgcgtttgc tcccgccgcg tgcgagttcg cgtatcggac gagcgcgttc
    11821 aagggcaaag accgctacgt gatcaccgag gtcgagtttc gcctgccaaa gcgcgtggac
    11881 agcatgccga tcaagtatgc ggagctcgcg aaggcactcg ggctgcaaga aggtgcaacg
    11941 gcctcgctcg cgagtgtgcg cgatacggtg atcgggctgc gccgcaagaa gggcatggtc
    12001 gtcgacgccg cagaccccga ttcgcgcagc gccgggtcct tctttacgaa tccgatcgtg
    12061 gctgcagagg tcgcagcgac gctcaccgac gcaccgatgt ggccgcagcc tgatggccgc
    12121 gtgaagctca gcgcgggctg gctgatcgag cgcgcgggtt tcacgaaggg caccgtgcgc
    12181 ggcaacgtcg gcatctcgag caagcacgcg ctcgcgctcg tcaatcgcgg tggcgccacc
    12241 gccgccgagc ttctggcgtt cgcgagagag atccaagaag ctgtacgcag caagctcggg
    12301 gtcgagctgc agatggaacc cgtgatcgta tgaagcatcc gttcgccggc aagcgcgtcg
    12361 cggtcgtcat gggcgggctc tccgccgagc gcgaggtgtc gctcaacaca ggcgcgggtg
    12421 tgctcgctgc gctgcaagag aagagctggg acgcggtcgg catcgattgg aaagagggca
    12481 cgagcttgcc ctcgctgctc gcggcgagcg gagcgcacgt cgtgtggaac gcgctgcacg
    12541 gtacgtacgg tgaggacggc gcggtgcagg gcctctgcgg ttgcatgctg ctgccttgca
    12601 cgggatccgg aatcctggcg agcgcactcg cgatggacaa ggtgatgtcg aagcggatct
    12661 tcgagagcaa cggcgtgccg acgccgcgct ggaagctcct gccgcacgat ggtcccgcgg
    12721 atggcagcga tgccgaagcg cttctcgccg atttcccgct tccgtgcgtc gtgaagcccg
    12781 cgaacgaggg cagctcggtc ggtgtgtcga tcgtcgagga gcgcggtcag ctcgcggcag
    12841 ccgtcgcaac tgcgcgtgcg tttcacggcc aagtgatcgt cgaggattac atcgcgggca
    12901 ccgaagtgtt cgtcggcatc ctcgatggcc gcgtgctcgg atccgtcgag gtcaggcccg
    12961 cgacgaagtt ctacgactac gaggcgaagt acaaacgcaa cgacacgcag tacttgatcc
    13021 cgccgcagct accggccgac gtgatcgcgc gaacggagca gctcgcactc gctgcgtaca
    13081 acgcgctcgg atgcagtggc cactcgcgcc ccgacttgcg catcacgaca gccggcgagc
    13141 cgtacgtgct ggaagtcaac acgttgccgg gcatgacgaa gacgagcctt ctgccgaaga
    13201 tcgcgaagtc gatcggcatg gactacggaa cgctgtgcga gcagatcctc gcgtcggcga
    13261 agtagctgcg ccgaaaatga aaagagccgc gagtgcggcc cttctcgtcg gtgatctctt
    13321 agatcacttg cagttcatgt cgtagacctt cgtccacgtc ttgcccttgt ccatcgacat
    13381 ctcaccccac atgtgcatgc cgaccttctt gtcggtcatg tcgccgtggt ccttgaacat
    13441 gccgtcgccc atgccgccct tggtgtccca cacggcctcg agcttgccgt ccttgaaatc
    13501 ggcggtgccg accatctggg taccgtcgtt catgacggcg acagcgcgcc acttaccgag
    13561 cttgctgtcg aaggtcatgt agttctccat cttcatggtg cccttcgcct tgccctcgcc
    13621 catcgtgccg gtcatcgact cgtgaatcca ccagccgtcg aggtccacct tgcgcgtgac
    13681 cgtgccggcg aacttcactt cggacttcat gtccatgccg agacccgtgc ccttgcacgt
    13741 cgcgcgtgca ccggcggcct tcagcgtgtc cttgatttcg gcgggcggct tcggcatctc
    13801 catcgccgcg cctgcctgca ccgtcgccgt cgcgccgccc ttcgcagcgc ccgcggccgc
    13861 atccgcgccg gtcttcgcag cgcccgtggc cgcgcccgca ccgctcttcg cagcgcccgt
    13921 cgccgtgccg gttgcagcac ccgcggtgcc ctttgcagcg cccgtcgcgc cgcccgcgtt
    13981 cacggtcgcg cctgccgacg ccgaaccttt tgccgtctgc gcgctggcca caccggccat
    14041 cgcaatcatc gagaacatcg caatcgagat cttcttcatc tgcctctcct cctcaagtgc
    14101 gcgcactata ctgaaattga aactgcacgc gtgatcgtgg gtctcataga cgacgtgatc
    14161 ggatctcgat tcacactctc gctgttcgtg agcgttgcct acttggcagc gtgtggcgga
    14221 acgccccgcg aaacagcgaa gcctgtcgta gcaaaaccgc acactgcagc cccacatcat
    14281 ctcgacgatc cggatctcaa tccgccgccg ccgaagaagc tgctctcgat cgagtggaag
    14341 agcgtcacgc tcacctcgga ggccgacgcg ctcgcgttgt ggaagacgat cgcgccgacg
    14401 ggtgacgact acaccgagaa gctcgacgag atccccgacg acggacccat cgcgacgcag
    14461 ctcgcactcg cgctgttgca cggcggtaac ttcagctgct ctccaacgcc gtcgcagtgc
    14521 ggtgcaccgg tcgatgtccc cgagccgatg ccgaccgcga cgtttgatga tccatgtctg
    14581 cgacgcatcc tcgcgatgtg gtcgctgcaa cagctcgatg acgacgacgt gcagtgggtg
    14641 cgcgattcgt tgaagacgat cgtcgcgatc ccgccgccgg aatcacagct cgtcatggcg
    14701 gcactcgatg tcgtgcctac gagcgagcaa gatctcaagc tcgagctgct ctcgatcgcg
    14761 cagagcgctg gacatcgcga gctcgcaaac ggcctcgtcg gatcgctcga cgaagcgcac
    14821 cttgtcgacg ccgcgcagaa gcatcacatg agtggcccac tcgacatgct ctccgcggaa
    14881 gcgcaccgcg cggtcttcat cgccgcgatt aacgacgcga agctgacgac gaatgcacgt
    14941 ctgcaagcga tcaccgagat cgtgacgagc gaagacaagc tctcgaagga gacgcgcgcc
    15001 actctcgcga ccgcaacgcg ctcgcccgat tgcaccgttg cagctgcggc tgcgcgcgca
    15061 ctcgtgcagc acggcgagaa gaagttcgcg ccggcgcgtc ccgcgaacag gtccagcgcc
    15121 gcgatgatgc gcgcgatgtg cgtgctcgcg agctacgagc agctgcaagg caacgacgag
    15181 gcgtcgtact tgctcggcta cgtaccggcg aagggtctcg aagtcgtgac ggtgacctac
    15241 gatcagtaca gcgacgccga cgacgacggc gacggcgacc cgcacacgca gcgcaccacg
    15301 aacattgtcc cgcgcaccga agtcgtgctg cccgagctcg aggatctcgt ccgcgcgatg
    15361 gcccactgca ctgggaccgt ctgccgctcg gacgaccgag aatttcgctt tacattcaag
    15421 ggagaccagc tccgacgagt cgaggtggtc gaacggccgc cttgcgtgcc aagataaccg
    15481 gcatgtcgcg ctggctcggc ctcgtcctgg tgctcggtct gacggcaacg ccggcgtacg
    15541 cgcaggtctt caaaccgaag acgggcaaga ccgcgaaggc caagagcgac aaggccgaga
    15601 aggccgagaa aaccgagaag gccgagcccg ccgagaagaa ggcgaagtcg cccggcaaga
    15661 agcgcgtcac gacgaagtcg aaaaagagct cgtcacgcga cagtgatcag gcgtcgtcct
    15721 cgggtcccaa agactcggac aaggacttcg tgaagatcac cgatgacgac gacgtcgagt
    15781 aggtgcatct gcgcaaagcg caaagggatc cggagtgcgg ccgaaaaatt gccccggtga
    15841 tcccgacatg agtatttcca catcatggat cgatccctcg gcccgagcgc agctcgtgcc
    15901 gcttcagcat cggcccgccg ggatgggacg aagcagcctc ctgcggcttc gcgcctaacc
    15961 gtcaagcctc gcggtaaccg ccgcaagacc cagccaggat cggtctggtc caagctcccc
    16021 aagcccgcgg tgatcgcgaa cgcatgctcg cgcgtgctgc gccgatcggt ccccgcgctg
    16081 atcggtctcg cggtcctcgg caccgtcggc ggcaccgcgt gggccggcta tcgattcgtc
    16141 acgcactcgc cgcgcttcgc ggtcacgacc atcgacgtca ccggcaatcg ccaggtcacg
    16201 accgacgaga tcatgggctc gcttcccgcg catgccggcg acaacgtgtt ctccgtcgat
    16261 ctcgacgcag tctcgcgcgc ggtcaagacg aatccctgga tcgcacaagc cgaagcgcac
    16321 cgcgtgttgc cgcacacgat cgcgatcagc gttcgcgagc acgtcgccgc tgcggtcgtc
    16381 gagctctcgg gcctctacct cgccgacgag caggggcatc cgttcaagaa agcagacctc
    16441 acgatcgacg agggcgccgg cctgccgatc atcaccggcc tcgatcgcgc acagtacggc
    16501 gcgaatcccg acgcaggcgc tgcccaggtt cgcagcgcgc tcgatgcact cgctcggtgg
    16561 aacgagaacg gcgatcgccc ttcgatcggc gagctgcacc tcgatccgca cggcaacctg
    16621 acgctcgtca cgtacgagca cgcgatcaag atccagctcg gtgttccgaa cgacttcctc
    16681 gccatgcgtc tcgccacgtt cgacgcgacg tggaacgagc tctcgagcag cgagcgctcg
    16741 cgcatgcgcg ccatccatct cgacgcccac tccgatcagg tcaccgtcgc cttcgcccca
    16801 gagaaagatc agtaacctca catggccaag cccaagagca acgagatcat cgtcggcctc
    16861 gacatcggca ccaccaagat cgcctgcatc gtcggcgagg tgacggagga gggtgtcgac
    16921 atcatcggca tcggaacgtc agcctccaag ggcctgcgcc gcggctgcgt cgtcaacatc
    16981 gacgcgacgg tcgcctcgat ccagcaagcg gtcgacgaag ccgagaatat ggcgggctgc
    17041 gagatctcgt cggtgtacgc cgcgatctcg ggcgcccacg tcaagggcct caactcgcac
    17101 ggcatcatcg ccgtcaagga caaggaagtc cgcgagagcg acatcgcccg cgtgatcgag
    17161 caggccaagg cggtcgcgat cccgatggac cgcgaggtgc tccacgtcct gccgcagcag
    17221 tacgtcgtcg acgaccagga cggcatccgc gacccgctcg gcatggccgg ggtgcggctc
    17281 gaggccaagg tccacatcgt cacgaccgcg gtgaccagcg cccagaacgt cgtgaagtgc
    17341 gccaa
//
LOCUS       DATLBZ010000259        15237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5499493, whole genome shotgun sequence.
ACCESSION   DATLBZ010000259 DATLBZ010000000
VERSION     DATLBZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15237
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5499493"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3032)
                     /gene="carB"
                     /locus_tag="VMZ53_13615"
     CDS             complement(<1..3032)
                     /gene="carB"
                     /locus_tag="VMZ53_13615"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HUS29543.1"
                     /translation="MPRRKDLESILIIGSGPIVIGQACEFDYSGTQACKALREEGIRV
                     ILINSNPATIMTDPELADATYVEPLTVEVLDKVLAKEKPTAILPTLGGQTGLNLAMAA
                     AKAGLLEKHGVELLGATVKTIEMAEDRELFKQAMERIGLACPKSVMVTSVEQARTIID
                     EIGFPAILRPSFTMGGSGGGIAYNKPDFDRMVQFALDQSPVHSVLVEESVLGWKEFEL
                     EVVRDKADNAVIVCSIENLDPMGVHTGDSITVAPAMTLTDKEYQRMRDASIAILREIG
                     VTTGGSNVQWAVDPKTGRMLVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     LKNDITRVTPASFEPTIDYVVVKWPRFAFEKFPSATPVLGPQMKSVGEAMSIGRTFRE
                     ALGKAIRSLETGRAGFDLPMHNMGDDLKTIERALAIASPDRLFQVGRAFQLGLTIERA
                     HELTRIDPWFLHHVHAIAMAEVEVSTKKVTELGPDLRRYKRLGMSDRRIAALTGATEA
                     QVRATRHAAGIRPVYKRVDTCGAEFESHTPYMYSSYEDECESKPTTKKKVIILGGGPN
                     RIGQGIEFDYCCVHAALALREQGIESIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLE
                     ICDKEKPWGVIVQFGGQTPLKLAVPLAAAGVPIIGTPADAIDRAEDRERFGEVMKKLG
                     LHAPRWGIARSLDEARKVAAEIGFPVMVRPSYVLGGRAMERVYDPRHLEDYFDRFLRA
                     SGKGGNDESATVGFPLLVDQFLDEAVELDVDVVADKTGAVVVGGVMEHIEEAGIHSGD
                     SACALPPYSLPADIVDEVKRQGRMLATELGVIGLMNVQFAVHDGEVYVLEVNPRASRT
                     IPFVSKAIGKPLAKIAAKIMAGQTLAELGLSEIEPRHVSVKEVVFPFVKFEGVDTILG
                     PEMRSTGEVMGIDSDFARAFAKSQLAVGMKLPTKGVAFLSVREQDKPALPAIARRLVN
                     LGFTLVATHGTAAYLKSHGMADVAGINKVLEGRPHCVDA"
     gene            complement(3101..3832)
                     /locus_tag="VMZ53_13620"
     CDS             complement(3101..3832)
                     /locus_tag="VMZ53_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29544.1"
                     /translation="MVFGLFSKERSLQKTIEKATNKLAQQPDRWGALEKLRDDGSEDA
                     LFGLCKRFGVVSQKGIEDEQEKEWVVDVLVEKGGASLEPVRRYMKSADQLSFPLRVLG
                     RIADHDKVVSICDEVFKAEPPGYARFPERRIDLIKWFGEWKGATEDEVIERITPYLQD
                     FDENVRFTAIDMLSRWTPAKIAPGLIDALVRPEEESGRIKRTIVEVLEKIKAPLGGRG
                     KEVQAAMTGTAADEFKVDGDVVKKR"
     gene            3880..4935
                     /locus_tag="VMZ53_13625"
     CDS             3880..4935
                     /locus_tag="VMZ53_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29545.1"
                     /translation="MRLCLVLLLAVMACGAKSKPRQTTPSDGQCFYDCKPEGQTDTSQ
                     ASPQPAAAPTKPGGKLTPAGEKAALLRQAADLLEKAGDSLASGNKNLAENLFSTAELL
                     VGPEALASLAGQFREGAPPRVNTPTKKFDTNIAAQSRAVGSSEEEDEADKVPPPPKVE
                     LGTLSGTLQIDGKPLTGAFGLVTLEPAGGKWKPRPAKGHVMAQRGREFNPHVMAISVG
                     STVSFPNFDTVFHNVFSTSPQAAFDLGIYKQGEAREYKFEKEGIIRLGCNLHANMSAY
                     LVVVSAPAYVVTDDKGNFKFNRLSPGKYKLKAWSEKSKQPITQDITIKTGANTANVGV
                     AADAPAGPPPDKFGGKR"
     gene            4932..6821
                     /locus_tag="VMZ53_13630"
     CDS             4932..6821
                     /locus_tag="VMZ53_13630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29546.1"
                     /translation="MKGSLVAIGALLLVGGGAALGYGVTRKPTGGMSPAAEQAMTSAV
                     DRLDSDVKAASASVKERVATLSTLLQVRAAVGTSPDTAKDMLARGELQFAPQAGEVVE
                     LGQLPSPDKPITLLIQPDGAMHAPHAGMIGSFTDVVDTKLVITEVTAVMSADPKRPSG
                     YLSVTRPLDLAPQIKSLAAAGINGRIAIGDQMLDIGSPQAGAQTIEKSLPSSPNIKVT
                     AAVPPKTGGMPMPLVAGGGIAAGLGLLLVVLGMMTKSSAAAASTQPQSQPQPQPSGLG
                     GAATQLSTPGVAAGTPSPIDAGSSMISPTNLGPGAMVGRWEVIKRLGSGGMADVFLAR
                     ARGDAGFEKTVAVKVMHPHLARNQRAVDHFLDEARLAARITHPNVVQILDLGKIGNDY
                     VIVMEFVEGVDLERLLASARAAERPVPIDVGLGILCRICDGLNAAHKALAPDGSPLNI
                     VHRDVKSANVLVSRQGGVKVVDFGIAKAAQQSHNTVAGETKGTPSMMAPEQRVGEQVD
                     VRADVYSVAAVGFEILTGHGVNLDLAALAHLGVENWPHLPLPSSLRPQLPPELDGLLL
                     GAMSFDRTKRPDDCTVLEAALEAIMKKHAMVCSDKDIARWVTSEMSALASGPGTEATL
                     SPSVR"
     gene            complement(6828..7637)
                     /locus_tag="VMZ53_13635"
     CDS             complement(6828..7637)
                     /locus_tag="VMZ53_13635"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS29547.1"
                     /translation="MMKVALVLIALAACTHPSTVTAPSARIERNVEFAPATTLDVYAP
                     LNATSAPVIVWFHGGALVEGDKQDEAAIAARFAEAGFVTIVPNYRLSPGVAHPAHVQD
                     AASAVAWSIAHARDYGGDSARMFVGGYSAGAYLAALLATDERYLAAHQQSPSALRGVI
                     LVSGFFWVERPGVAPDRPTSVWGADAGAWPDASPAHHLHDDMRPHLIIYADGDDEWRR
                     QQNTEYAETLKSAGQLVMVHDRNHTTIHTTLSAPKNLVLRQLFDFVRRESQ"
     gene            complement(7634..8278)
                     /locus_tag="VMZ53_13640"
     CDS             complement(7634..8278)
                     /locus_tag="VMZ53_13640"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HUS29548.1"
                     /translation="MGTTLSVAPHRLLDAGAFVTRFAQPMAQLTAPPQLADLLTSGAA
                     PLSSSETVRSEVRQLLREGGFKPSGRSKPASEYLVAAHAEARFPRINAAVDACNVASL
                     FSGLPISLIDLDRVVGGLEIRVCPPNTTYVFNPSGQVIDASGLLAVFDAEGPTGTPVK
                     DAQRTKTHDDTRTTLAVVWGTNALPGRTEQATRWYRELVAMIPGATNEDVEVRR"
     gene            8337..10340
                     /locus_tag="VMZ53_13645"
     CDS             8337..10340
                     /locus_tag="VMZ53_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29549.1"
                     /translation="MPAAGEASRPTRLGRYELITRIGQGGMAEVQLALQRGPAGFEKL
                     VVVKLVHNNLASQKAFVDMLLDEARVAALVKHPNVVDIYDLGQAEGRFFIAMEYLEGE
                     PLLAVLRAGREGKRLDPLSTGRLIADTAEGLDAAHELRSMSGERLELVHHDISLGNIV
                     VLYNGQVKLVDFGVAKATQNAAGSKAKVQGKFSYMAPEKLKGAPGDRRSDIFSLGCVM
                     WEALTLKRLFRGGNDAETMKQVLELAIQPPSKVNGDVPPEYDAIVMRSLDRDPDKRHG
                     TAKELAVEIEELLRKRGYGAKNDRVAKYMQETFKSHIDARKKLVQEVVSKGSASAEVL
                     DAAFSDPVTLASGSPVTPGSQPGTDFSGKYRRVTPAAGVPVVTAAWADASAKTTISTP
                     PVHPMFDGGKATELDGMPLGDGDDVTVKAKPAGDANTLVEKPISPIDRAKAFALAVWG
                     DKTKRMIALGAGGVLLLILVIAIAAGGGDGPKAAAKQNDGKGSGSAAPIVDNGSDRVV
                     ADNTGSGSAEATGSAGSAAVVPENGSGAGSDDVIEMHEDGTGSGSATKPNDRGSNDRR
                     NNDRTNDRHIDHHIDRPKVDVKETVNQATILHVRGDNKAALTLLQTAKAAQPGYAPTY
                     RVLGNVYKKLGNNSGAKSAFQTYVKLAPKAGDVDTIKDEIKKL"
     gene            10337..11203
                     /locus_tag="VMZ53_13650"
     CDS             10337..11203
                     /locus_tag="VMZ53_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29550.1"
                     /translation="MRFAIVIVIAAFVLAATVTAGNRKVFVLPVDGNAPNAQRIGIND
                     AIVSLAKTGMDGDVSSGDTTFNETAAAVGCTPDQASCAETVMTTLAVDELVWGTAQTE
                     NGSTTVTVHRMAKGESPREQKSVIPESANGLAVSTGLTPLFSRDQGIGSAEGSDAGSG
                     SAAVERPRPKSFFDTTERKLGVGLGAASVTCLIIGFSLWSSESGLQDQIDAHPRDTLG
                     QLQDLHELEDRAASKALWGNVFVVLGVAAGGVSGYYFWKDHKNRSERTTVIAPVPTAD
                     GTGVKMVLGGRW"
     gene            11200..12198
                     /locus_tag="VMZ53_13655"
     CDS             11200..12198
                     /locus_tag="VMZ53_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29551.1"
                     /translation="MKRAGRSAGAMGAMVGLIIAASAVLWSCRTDRVSDGYACNDNND
                     CDADRVCNTGYCVVGQRPVDARLQDAFIVDAATCPDVCKNTCNLTTRDCPITGTGSGN
                     ITCPTGWNCDISCPTAGACGTISCTSAADCDIHCTADDACLGITCGTTDCKVDCTGVN
                     ACGNIGCTTGKCEALCTGSDACGTLTCTSGDCTQNCSGGGGAQVCGNLSCTTGDCSRT
                     CTGAGACGSMSCNGNTASKCSETCSGGNAACGPMTCTTSKCTATCTGAAPSSCGNVNC
                     DTSCGCEVNCDLTTNACPASMMCATPGARYCSLTGASGSRCTLDGTGGIQQCNACQ"
     gene            12189..12716
                     /locus_tag="VMZ53_13660"
     CDS             12189..12716
                     /locus_tag="VMZ53_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29552.1"
                     /translation="MPMKSTTVIVAVALVALSIAACFVHRPSDELVCSSDADCMDGRK
                     CTSGYCVVPQCPSDCDGCDEAGKSCTMNCSSNDDCNSVNCPSGWTCIINCIGDGACDD
                     VSCSSGSHCTITCTGDGACNDISCRDACECDLLCSGTACDAISCPQTSGGIKCTTDGT
                     IGSPCLSTAAGCTKC"
     gene            12745..13251
                     /gene="greA"
                     /locus_tag="VMZ53_13665"
     CDS             12745..13251
                     /gene="greA"
                     /locus_tag="VMZ53_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HUS29553.1"
                     /translation="MSDKFPMTPSGYATMKAHLYRLKNVDRLANSKAIEIARGHGDLS
                     ENADYSAAKEEQGLIEAKIRELEAKIAMAEVIDPTKLSGSRVKFGATVTIEDSESGET
                     QRYTIVGEHEADIKKGRISVGAPVARALIGKEIGDAVTVTTPKGKREYEVTEVEWLEV
                     PPLEEISQ"
     gene            13248..13448
                     /locus_tag="VMZ53_13670"
     CDS             13248..13448
                     /locus_tag="VMZ53_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29554.1"
                     /translation="MKRALVICLLLVGCESGEGERCQVDSDCANGLVCNKAKNVCTTG
                     KGGDLDASVPDGPPADAVPDAP"
     gene            complement(13445..14503)
                     /locus_tag="VMZ53_13675"
     CDS             complement(13445..14503)
                     /locus_tag="VMZ53_13675"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUS29555.1"
                     /translation="MRIRAILAAATASSVAVAIAAAGNGKVIRIEQAPSREVFVPAGS
                     FWMGVGEADAETIAGQCEMLFDPQSQILAINGRVTLCTGYRDELAQMTLRRVFISAFA
                     IDRNEVSVRDFRECITAGVCSLDPLIGGDERYIQDRWPIVNVTWFEAQEYCRWRGGRL
                     PTEAEWERAARGDDPDEARAAEKVWPWCDEAIKDQTDKLCVERTRDFNHGQPRAQAMR
                     DIDRVGDPLHLLGDPDDVDGFALIAPPGSYPWGEGPYGTRDQAGNVAEWVADARGTGE
                     MALGYRSLAGCTETEDGTLCINPKREGNDRDLRIVRGGSWRQPSFVSRSNLRDPYGVV
                     YEPNRRFSHVGFRCARPL"
     gene            complement(14524..>15237)
                     /locus_tag="VMZ53_13680"
     CDS             complement(14524..>15237)
                     /locus_tag="VMZ53_13680"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS29556.1"
                     /translation="GLVAMPEVSAQIGTWLASKHARTRAAACEALGSFAPADAKKWLV
                     KGMSDRDASVRAACTSSVRDPGSVRAKLRVLAKDRDIATRAAAVAHLARLPDDVVSVD
                     DDPASQVRAAYAAAVTPTHDLSTLLADRDPDVRAAAWQTHARGGAPTLKAEAADAADD
                     PSPFVRRALIPALDDDETISRLARADVDGDVRRDALVRWAERRGRASVADSLLTNFAL
                     APPGSRDRVRTVLAWLLAR"
BASE COUNT         2721 a         4962 c         5056 g         2498 t
ORIGIN      
        1 gcgtcgacgc agtgcgggcg gccttcgagc accttgttga tgccggcgac gtcggccatg
       61 ccgtggctct tgagataggc ggcggtgccg tgcgtggcga cgagcgtgaa gccgaggttg
      121 acgaggcggc gcgcgatcgc aggcagcgcg ggcttgtctt gctcgcgcac ggagaggaag
      181 gcgacgccct tcgtcggcag cttcatgcct accgcgagct gcgactttgc gaacgcgcgc
      241 gcgaagtcgg agtcgatgcc catgacttcg cccgtcgagc gcatctcggg gccgaggatc
      301 gtgtcgacgc cctcgaactt gacgaacggg aacacgacct ctttgacgga gacgtggcgc
      361 ggctcgattt cggagaggcc gagctcggcg agcgtctggc cggccatgat cttcgcggcg
      421 atttttgcga gcggcttgcc gatcgctttt gagacgaatg ggatcgtgcg cgacgcgcgc
      481 gggttgacct cgaggacgta gacctcgcca tcgtggacgg cgaactgcac gttcatgagg
      541 ccgatcacgc cgagctccgt cgcgagcatg cggccctggc gtttgacctc gtcgacgatg
      601 tcggcgggca gcgagtatgg cggcagcgcg caggcggaat cgccggagtg gatgccggct
      661 tcttcgatgt gctccatcac gccgccgacg acgaccgcgc cggtcttgtc tgcgacgacg
      721 tcgacgtcga gctcgaccgc ttcgtcgagg aattggtcga caaggagcgg gaagccgacg
      781 gtcgcggatt cgtcgttgcc gcctttgccc gacgcgcgaa ggaagcgatc gaagtagtcc
      841 tcgaggtgac gagggtcgta gacgcgctcc atcgcgcgac cgccgaggac gtacgacggt
      901 cgaaccatca ccgggaagcc gatttcggcg gcgaccttgc gcgcttcgtc gagcgagcga
      961 gcgatgcccc agcgcggcgc gtggaggccg agcttcttca tgacttcgcc gaagcgctcg
     1021 cgatcttctg cgcgatcgat cgcgtcggcg ggcgtgccga tgatcgggac gccggcagcg
     1081 gcgagcggta cggcgagctt gagtggggtc tggccgccga attggacgat cacgccccac
     1141 ggcttttctt tgtcgcaaat ctcgaggacg tcctcgagcg tgagcggctc gaagtagaga
     1201 cgatcggacg tgtcgtaatc cgtcgagacc gtctccgggt tgcagttgac catgatcgat
     1261 tcgatgcctt gctcgcgcag cgcgagggcg gcgtgcacgc agcagtaatc gaactcgatg
     1321 ccttggccga tgcggttcgg accgccgccg aggatgatca ctttcttctt cgtcgtcggt
     1381 ttcgattcgc actcgtcttc gtacgagctg tacatgtacg gcgtgtgcga ctcgaactcg
     1441 gcgccgcacg tgtcgacgcg cttgtagacc ggacgaatgc cagcggcgtg gcgagtggct
     1501 cgcacttgcg cttcggtcgc gccggtcagt gcggcgatgc gacggtcgga catgccgagc
     1561 cgcttgtagc gacggaggtc ggggccgagc tcggtgacct tcttcgtcga gacttcgacc
     1621 tcggccatcg cgatcgcgtg cacgtgatgg aggaaccacg gatcgatgcg cgtgagctcg
     1681 tgtgcgcgtt cgatcgtcag gccgagctgg aaggcgcgac cgacttggaa caggcgatcg
     1741 ggcgatgcga tcgcgagcgc gcgttcgatc gtcttcaggt cgtcgcccat gttgtgcatc
     1801 ggcagatcga agcccgcgcg gcccgtctcg agtgagcgga tagctttgcc gagcgcttcg
     1861 cggaacgtgc gaccgatcga catcgcctcg ccgaccgact tcatctgcgg gccgagcacc
     1921 ggcgtcgccg acgggaactt ctcgaacgcg aagcgcggcc acttcacgac gacgtagtcg
     1981 atcgtcggct cgaacgatgc gggcgtcacg cgcgtgatgt cgttcttgag ctcgtcgagc
     2041 gtgtaaccga tggcgagctt cgccgcgatc ttcgcgatcg ggaagcccgt cgccttcgac
     2101 gcgagcgccg aggaccgcga cacgcgcggg ttcatctcga tcacgagcat gcggcccgtc
     2161 ttcggatcga cggcccactg gacgttggag ccgccggtcg tcacgccgat ctcgcgaagg
     2221 atcgcgatcg aggcgtcgcg catgcgctgg tactctttgt ccgtcagcgt catcgcgggc
     2281 gcgaccgtga tcgaatcgcc cgtgtgcacg cccatcggat cgagattctc gatcgagcag
     2341 acgatcaccg cgttgtcggc cttgtcgcgc acgacctcga gctcgaactc tttccagccg
     2401 aggaccgatt cttcgacgag caccgagtgc accggcgatt gatcgagcgc gaactgcacc
     2461 atgcgatcga agtcgggctt gttgtacgcg atgccgccac ccgagccgcc catcgtgaac
     2521 gagggccgca ggatcgcggg gaagccgatc tcgtcgatga tcgtgcgcgc ctgctcgacg
     2581 ctcgtgacca tcaccgactt cgggcatgcg aggccgatgc gctccatcgc ttgcttgaac
     2641 agctcgcgat cctcggccat ctcgatcgtc ttgaccgtcg cgccgagcag ctcgacgccg
     2701 tgcttctcga gcaggcccgc cttcgccgcg gccatcgcga ggttcagacc ggtctggccg
     2761 ccgagcgtcg gcaggatcgc ggtcggcttc tctttcgcga ggaccttgtc gagcacctcg
     2821 accgtgagcg gctcgacgta ggtggcgtcg gccagctccg ggtccgtcat gatcgtggcc
     2881 gggttgctgt tgatcagaat gacgcgaatg ccctcctcgc ggagcgcctt gcacgcctgc
     2941 gtgcccgagt aatcgaactc gcacgcttga ccgatcacga tcggcccgga gccgatgatg
     3001 agaatcgact cgagatcctt ccgacgtggc atcgggacgt tcgtttagca catcccgaga
     3061 gatctacatc agctgatcgc cggcgattta ccgacgaccg ctatcgcttc ttcacgacgt
     3121 cgccgtcgac cttgaactcg tcggctgccg taccggtcat cgcggcctgg acttccttgc
     3181 cgcggccgcc gagcggcgcc ttgatcttct cgagcacctc gacaatcgtg cgcttgatgc
     3241 ggccggactc ttcctcgggg cgaacgagcg catcgatgag gcccggagcg attttcgcag
     3301 gcgtccacct cgacagcata tcgatcgcgg tgaatcggac attctcgtcg aagtcttgca
     3361 ggtacggcgt gatgcgttcg atcacctcgt cctcggtcgc gcctttccac tcgccgaacc
     3421 acttgatgag atcgatgcgg cgctcgggaa aacgcgcgta gcccggcggc tccgccttga
     3481 agacttcgtc gcagatcgag acgaccttgt cgtgatcggc gatgcggccg agcacgcgaa
     3541 gcgggaacga gagctggtct gcggacttca tgtagcggcg cacgggctcg agcgaggcac
     3601 cgcctttttc gacgagaacg tcgacgaccc attccttctc ttgctcgtcc tcgatgccct
     3661 tctgcgacac gacgccgaag cgcttgcaga ggccgaacag tgcgtcttca ctgccgtcgt
     3721 cacggagctt ttcgagggct ccccagcggt cgggctgttg cgcgagcttg ttcgtcgcct
     3781 tctcgatcgt cttctgcagt gatcgctctt tggaaaacag cccgaatacc atgcgtaggc
     3841 tccgaaaatc tgaagggaag ctagcgtaca atacgctcga tgcgactctg tctcgtgctc
     3901 ctgcttgccg taatggcttg cggagccaaa agcaagccca ggcagaccac gccttccgac
     3961 ggccagtgct tttacgactg caagccggaa ggccagacag acacgtcgca agcgtcaccg
     4021 cagcccgcgg cagcgcccac gaaacccggc ggcaagctca ctcccgccgg cgaaaaagca
     4081 gcgttgctcc gacaagctgc cgatctgctc gagaaggccg gcgactcgct cgcgtcgggt
     4141 aacaagaacc tcgccgagaa tttgttctcg accgcggagc tgctcgtcgg tcccgaagcg
     4201 ctcgcatcgc tcgccggtca attccgcgaa ggcgcgccgc cgcgcgtgaa tacgccgacg
     4261 aagaagttcg acacgaacat cgccgcgcag tcgcgagcgg ttggctcgtc ggaagaagaa
     4321 gacgaagcag acaaggttcc gccgccgccc aaagtcgagc tcggcacgct cagcggcacg
     4381 ctgcagatcg acggcaagcc gctgaccggt gcgttcggtc tcgtcacgct cgagcccgcg
     4441 ggtggcaagt ggaagccgcg gccggcgaag gggcacgtga tggcgcagcg cggccgcgag
     4501 ttcaacccgc acgtgatggc gatctcggtc ggctcgacgg tgtcgttccc aaacttcgac
     4561 accgtgttcc acaacgtgtt ctcgacgtca ccgcaagccg cgttcgacct cggcatctac
     4621 aagcaaggcg aagcgcgcga gtacaagttc gagaaagagg gcatcattcg cctcggctgt
     4681 aacctgcacg cgaacatgag cgcatacctc gtggtggtat ccgcgcccgc gtacgtcgtc
     4741 accgacgaca aaggcaactt caagttcaat cgcctctcgc ccggcaagta caagctcaag
     4801 gcgtggagcg agaagagcaa gcagccgatc acgcaagaca tcacgatcaa gaccggcgcg
     4861 aacaccgcca acgtcggcgt tgctgctgac gcgcctgccg gtccgccacc cgacaagttc
     4921 ggaggcaagc gttgaagggc tcactcgtcg cgatcggtgc gctgctgctc gtcggtggcg
     4981 gcgcggcgct cggctacggg gtgacgcgca agccgaccgg cgggatgtcg cccgcggccg
     5041 agcaagcgat gacgagcgcg gtcgatcgcc tcgatagcga cgtcaaagca gcgagcgcgt
     5101 cggtgaaaga gcgcgtcgca acgctctcga cgttgctgca agtgcgcgcc gccgtcggca
     5161 cgagccccga caccgcgaaa gatatgctcg cgcgcggcga gctgcagttt gcaccgcaag
     5221 ccggcgaggt cgtcgagctc ggtcagctgc cgtcgcccga taaaccgatc acactgctca
     5281 tccaacccga cggcgcgatg catgcgccac acgcgggcat gatcggaagc ttcaccgacg
     5341 tcgtcgatac aaagctcgtc atcaccgaag tcaccgccgt catgtcggcc gatccgaaga
     5401 ggccgtccgg ctacctcagc gtcacgcgcc cgctcgacct tgcgccacag atcaagtcgc
     5461 tcgccgcggc aggcatcaac ggccggatcg cgatcggcga tcagatgctc gacatcggtt
     5521 caccgcaggc cggcgcgcag acgatcgaga agtcgctgcc gtcgtcgccg aacatcaagg
     5581 tcacggccgc ggtgccgccg aagaccggcg gaatgccgat gccgctcgtc gcaggtggcg
     5641 gcatcgccgc agggcttggt ctcttgctcg tcgtgctggg gatgatgacg aagagcagcg
     5701 cggccgcggc aagcacgcag ccgcagtcgc agccgcagcc gcagccgagc gggctcggcg
     5761 gcgcagcgac gcagctctcc acgccgggtg tcgccgcggg aacgccgtcg ccgatcgatg
     5821 cgggtagctc gatgatctcg ccgacgaacc tcggtccggg cgcgatggtc ggccgctggg
     5881 aagtcatcaa gcgcctcggc tcgggcggca tggccgacgt gttcctcgct cgcgcgcgcg
     5941 gtgacgccgg cttcgagaag acggtcgccg tcaaagtcat gcacccgcat ctcgcgcgca
     6001 accaacgcgc cgtcgatcac ttcctcgacg aagcgcgact cgccgctcgc atcacgcatc
     6061 ccaacgtcgt gcagattctc gacctcggca agatcggcaa cgactacgtc atcgtcatgg
     6121 agttcgtcga aggcgtcgat ctcgagcgct tgctcgcctc cgcccgcgcc gccgaacgcc
     6181 cagtcccgat cgacgtcggc ctcggcatcc tgtgccgcat ctgcgacggt ctcaacgccg
     6241 cgcacaaggc gctcgcgccc gatggctcgc cgctcaacat cgtccaccgc gacgtgaaga
     6301 gcgcgaacgt cctcgtctcg cgtcaaggcg gcgtcaaagt cgtcgacttc gggatcgcga
     6361 aggccgcgca gcaatcgcac aacacggtcg cgggagagac caaagggacg ccgtcgatga
     6421 tggcgcccga gcagcgcgtc ggcgagcaag tcgacgtgcg cgcggacgtc tactccgtcg
     6481 ctgcggtcgg cttcgaaatc ctcaccggcc atggcgtgaa cctcgacctc gctgcgctcg
     6541 cgcacctcgg cgtcgagaat tggccgcact tgccgctccc ttcgtcgctg cgcccgcagc
     6601 taccgcccga gctcgacggc ctattgctcg gcgcgatgtc gttcgatcgc acgaagcgtc
     6661 ctgacgactg cacggtgctc gaagcggcgc tcgaagcgat catgaagaag cacgcgatgg
     6721 tctgcagcga caaagacatc gcgcgctggg tcacgtcgga gatgtccgcg ctcgcgagtg
     6781 ggccggggac cgaagcgacg ctcagcccga gcgtccgctg atactgccta ctgcgattcg
     6841 cgccggacga agtcgaagag ctggcgcagt acgaggttct tcggcgcgct gagcgtcgta
     6901 tggatcgtcg tgtggtttcg atcgtgaacc atcacgagct ggccggcgct cttcagcgtt
     6961 tcggcgtact cggtgttctg ttggcggcgc cattcgtcgt caccgtcggc gtagatgatc
     7021 aggtgaggtc gcatgtcgtc gtgaaggtga tgggcgggtg aagcgtcggg ccacgcacct
     7081 gcgtcggctc cccagacgga ggtcggccga tcgggcgcga cgccggggcg ctcgacccag
     7141 aagaagccgc tgacgaggat cacgccgcga agagcggacg gcgattgctg gtgcgccgcg
     7201 aggtaacgct cgtcggttgc gaggagcgca gcgaggtacg cgcccgccga gtagccgccg
     7261 acgaacatgc gcgctgaatc gccgccgtag tcgcgcgcgt gcgcgatcga ccatgcgacc
     7321 gcggacgctg cgtcttggac atgcgcggga tgcgcgacgc cgggcgacag gcggtagttc
     7381 ggcacgatcg tcacgaagcc cgcttcggcg aaccgcgctg cgatcgctgc ttcgtcttgc
     7441 ttgtcgccct cgacgagcgc gccgccgtga aaccacacga tgaccggcgc agaggtcgcg
     7501 ttcagcggtg cgtagacgtc gagcgtcgtc gccggcgcga attcgacgtt gcgttcgatg
     7561 cgagcagagg gggcagtcac ggtggacggg tgggtgcatg ccgcgagcgc gatcagcacg
     7621 agcgcaacct tcatcatcgt cgcacctcca cgtcctcgtt tgtcgcgccg ggaatcatcg
     7681 cgacgagctc acgataccag cgcgtcgctt gctcggtgcg ccccggaagc gcgttggttc
     7741 cccacacgac ggcgagcgtc gtgcgcgtat cgtcgtgcgt cttcgtgcgc tgcgcatctt
     7801 tgaccggcgt gcccgtcgga ccttcggcgt cgaacacggc gagcaggcca ctcgcgtcga
     7861 tcacttggcc cgatgggttg aacacgtagg tggtgtttgg cggacacacg cggatctcga
     7921 gaccaccgac gacgcggtcg agatcgatca gcgagatcgg caggccggag aacagcgagg
     7981 cgacgttgca tgcgtcgaca gcggcgttga tccgaggaaa gcgagcttcg gcgtgggccg
     8041 ccacgaggta ttccgaggct ggtttgctgc ggcccgacgg cttgaaaccg ccctcgcgca
     8101 agagctgccg cacctcggaa cgcactgttt cggacgacga aagtggtgct gctcccgatg
     8161 tgagcaggtc ggcgagctgc ggcggcgcag ttagttgcgc catcggctgc gcaaaacgcg
     8221 tcacgaatgc gcctgcatcg agcagccgat gcggtgcgac cgagagggtg gttcccatac
     8281 ccacacatta gccaggcgcg caccctcgca aacccaagct gcgatacaat gttttggtgc
     8341 ccgcagcggg ggaggcttcg cgcccaacca ggcttggacg ctatgagctg atcacccgga
     8401 tcggccaggg cggaatggcc gaggtgcagc ttgcgctgca acgtggtccg gccggcttcg
     8461 agaagctcgt ggttgtgaag ctcgtgcaca acaacctcgc gagtcagaag gcattcgtcg
     8521 acatgttgct cgacgaggcg cgcgtcgcgg cactcgtgaa gcatccgaac gtcgtcgaca
     8581 tctacgacct cgggcaggcc gaaggccgct tcttcatcgc gatggagtac ctcgagggcg
     8641 agccgctgct tgccgtgttg cgcgccggtc gcgagggcaa gcgactcgat ccgctgtcga
     8701 cgggccgtct gatcgcggac accgccgaag gactcgacgc cgcacacgag ctgcggagca
     8761 tgagcggcga gcgactcgag ctcgtccatc acgacatctc gctcggcaat atcgtcgtgc
     8821 tctacaacgg gcaggtgaag ctcgtcgact tcggtgtcgc gaaggcgacg cagaacgcgg
     8881 ccggctcgaa ggcgaaggtc cagggcaaat tcagctacat ggcgcccgag aagctcaagg
     8941 gcgcgcccgg cgatcggcgc agcgatattt tttcgcttgg ctgcgtgatg tgggaagcgc
     9001 tcacgctgaa gcgcttgttc cgcggcggca acgacgccga gacgatgaag caggtcttgg
     9061 agctcgcgat ccagccgccg tcgaaggtga acggcgacgt gccacccgag tacgacgcga
     9121 tcgtcatgcg gtcgctcgat cgcgatcccg ataagcggca tggaacggcg aaggagctcg
     9181 cggtcgagat cgaagagctc ctgcgcaagc gcggctacgg cgcgaagaac gatcgcgtcg
     9241 cgaagtacat gcaggagacg ttcaagtcgc acatcgacgc gcgcaagaag ctcgtgcagg
     9301 aagtcgtgag caaaggctcc gcgagcgctg aagttctcga cgcggcgttc agcgatccgg
     9361 tcacactcgc atcgggctcg ccggtgacac ccggctcgca gcccggcacc gacttctctg
     9421 gaaagtaccg gcgcgtgacg cccgcagcgg gcgtgcctgt cgtgaccgca gcgtgggcgg
     9481 acgcgagcgc gaagacgacg atctcgacgc caccggttca cccgatgttc gacggcggca
     9541 aggcaaccga gctcgacggc atgccgcttg gcgacggcga cgacgtcacc gtcaaggcga
     9601 agcctgcggg ggatgcgaac acgctcgtcg agaaaccgat cagcccgatc gaccgcgcga
     9661 aggcattcgc ccttgccgtg tggggcgaca agacgaagcg gatgatcgcg ctcggcgcgg
     9721 gcggcgtctt actcctcatc ctcgtcatcg cgatcgcggc gggcggcggt gatggaccga
     9781 aggcggcggc aaagcaaaac gacggcaagg gcagcggcag cgctgcacct atcgtcgaca
     9841 acggcagcga tcgcgtggtc gccgacaata cggggagcgg cagcgccgag gcgacaggca
     9901 gcgcgggctc agccgccgtc gtgcccgaga acggcagcgg cgccggcagt gacgacgtca
     9961 tcgaaatgca cgaggacggc accggctcgg gaagtgcgac gaagcccaac gatcgcggca
    10021 gcaacgatcg ccgaaacaac gatcgcacga acgaccgcca catcgaccat cacatcgacc
    10081 gtccgaaagt ggacgtcaaa gagacggtca accaggcgac cattctgcac gtgcgaggcg
    10141 ataacaaagc tgcgctcaca ctgctgcaga ccgcaaaggc cgcgcagccc ggctacgcgc
    10201 cgacctatcg cgtgctcggc aatgtctaca agaagctcgg caacaacagc ggcgcgaagt
    10261 ccgcgttcca gacctacgtg aagctcgcgc cgaaggccgg cgacgtcgac acgatcaaag
    10321 acgaaatcaa gaagctatga gatttgcgat cgtcatcgtc atcgctgcct tcgtgctcgc
    10381 cgcgacggtt accgccggaa accggaaagt gttcgtgctg cccgtcgacg gcaacgcgcc
    10441 gaatgcgcag cgaatcggga tcaacgacgc gatcgtgagc ctcgcgaaga ccggcatgga
    10501 tggcgatgtc tcgtcgggcg acacgacatt caacgaaact gccgccgccg tcggctgcac
    10561 gccggatcag gcgagctgcg ccgagactgt catgacgacg ctcgctgtcg acgagcttgt
    10621 ctggggcacc gcgcagacgg agaacggatc gacgacggtc accgtgcatc gcatggcgaa
    10681 aggtgaatcg ccgcgcgagc aaaagagcgt tatcccggag agtgcgaacg ggcttgcggt
    10741 atcgacgggc ctcacgccgc tgttttcgcg cgatcagggc atcggctccg cggaaggctc
    10801 cgacgccggc tccgggtcgg cggccgtcga gcgtccccgg ccgaaatcgt tcttcgacac
    10861 gacggagcgc aagctcggcg ttgggctcgg tgcggcgagc gttacgtgcc tgatcatcgg
    10921 cttctcgctg tggtcgagcg aatcgggtct gcaggaccaa atcgacgcgc atccacgcga
    10981 cacgctcggg caactgcaag acctccacga gctcgaggat cgcgctgcaa gcaaggcgct
    11041 gtggggcaac gtcttcgtcg tgctcggtgt cgccgccggc ggcgtcagcg gctactactt
    11101 ctggaaggac cacaagaatc gcagcgagcg gacgaccgtt atcgctccgg tgccgaccgc
    11161 agacggaacc ggcgtgaaga tggtcctcgg aggacgctgg tgaaacgcgc gggtcgcagc
    11221 gcgggtgcga tgggcgcgat ggtcggcttg atcatcgccg catcggccgt actgtggagc
    11281 tgccgcaccg accgcgtctc cgacgggtac gcgtgcaacg acaacaatga ctgcgacgcg
    11341 gaccgcgtct gcaacaccgg ctactgcgtc gtcggtcaac gccccgtcga tgcgcgcctg
    11401 caagacgcat tcatcgtcga tgcggctacc tgccctgacg tgtgcaagaa cacctgcaat
    11461 ctgacgacgc gcgactgtcc gatcaccggc acgggcagcg gcaacatcac gtgccccacc
    11521 ggatggaact gcgatatctc gtgcccgacc gcgggggcat gcggaacgat cagctgcacg
    11581 agtgcggccg attgcgacat ccactgcact gcagacgacg cgtgcctcgg catcacgtgc
    11641 gggaccaccg actgcaaagt cgactgcacc ggcgtcaacg cgtgcggcaa catcggctgc
    11701 accaccggca agtgcgaggc cctgtgcacg ggctccgacg catgcggaac gctgacctgc
    11761 acgtctggcg attgcacgca gaattgctcc ggcggcggcg gagctcaggt gtgcggcaac
    11821 ctctcgtgca cgacgggcga ctgctcgcgc acgtgcacgg gcgctggagc gtgcggctcg
    11881 atgtcgtgca atggcaacac cgcgtcgaag tgctccgaga catgcagcgg cggtaatgcc
    11941 gcgtgcggtc caatgacgtg cacgacgtcg aagtgcactg caacgtgtac cggtgctgcg
    12001 ccgtcgtcat gcggcaacgt caactgcgat acctcctgtg gctgcgaggt gaactgcgac
    12061 ctcacgacga atgcatgtcc ggccagcatg atgtgtgcga cgcccggcgc gcgctactgc
    12121 agcctcaccg gtgcaagcgg gtcgcggtgc actctcgacg gtacgggcgg catccaacag
    12181 tgcaacgcgt gccaatgaaa tcgacgaccg tcatcgtcgc tgtcgcgctc gtcgcgctgt
    12241 cgatcgcagc gtgcttcgtc catcgtccat cggacgagct cgtctgctcc tcggatgcgg
    12301 actgcatgga cggccgcaag tgcacgagtg gctattgcgt cgtgccgcag tgcccatcgg
    12361 attgcgacgg ctgcgacgaa gcgggcaagt catgcacgat gaattgctcg tcgaacgatg
    12421 actgcaacag cgtgaattgc ccgagcggct ggacatgcat catcaactgc attggcgatg
    12481 gcgcatgcga cgacgtctcc tgctcgagcg gctcgcattg cacgattacg tgcacgggcg
    12541 acggcgcctg caatgacatc tcctgccgcg acgcctgcga gtgcgacctt ctctgctccg
    12601 gcacggcatg cgacgccatc agctgcccgc agaccagcgg cggcatcaaa tgcacgaccg
    12661 acggaaccat cggctcccca tgcctttcga ccgccgccgg ttgcacgaag tgctgagccg
    12721 cagtacgact gtccccctgc ggccatgagc gataaattcc cgatgacccc ctcgggttac
    12781 gcgacgatga aggcccatct gtaccggctg aagaacgtcg accggctcgc gaacagcaag
    12841 gctatcgaga tcgctcgcgg ccacggcgac ctgtcggaaa acgccgacta cagcgccgcc
    12901 aaagaggaac aggggctaat cgaggccaag attcgcgagc tcgaagcaaa aatcgcgatg
    12961 gccgaggtta tcgatccaac caaattgtcg ggttcgagag tcaaattcgg ggcaaccgtg
    13021 actatcgagg acagcgagag cggcgagacc cagcgctaca cgatcgtcgg cgagcatgaa
    13081 gccgacatca agaagggccg gatctcggtt ggcgcgcctg tcgcgcgtgc gctcatcggc
    13141 aaagagatcg gcgacgccgt taccgtgacg acccccaagg gcaagcgcga gtacgaggtc
    13201 accgaagtcg agtggctcga agttccaccg ctcgaggaga tttcccagtg aagcgtgccc
    13261 tagttatttg tctcttgctc gttggctgtg agtcgggtga aggcgagcgc tgccaagtcg
    13321 actccgactg cgcgaacggc ctcgtctgca acaaagcgaa gaacgtgtgc acgaccggaa
    13381 agggcggcga tctcgatgcc tccgtgcccg atgggccgcc cgccgacgct gttccggacg
    13441 cgccctagag cggacgcgcg cagcggaagc cgacgtgcga gaaccgccgg ttcggctcgt
    13501 acacgacgcc gtatggatcg cgcagattcg agcgggacac gaagctcggc tgccgccacg
    13561 atccgccgcg aacgatgcgt agatcgcgat cgttgccttc gcgcttcggg ttgatgcaca
    13621 gcgtgccgtc ctcggtctcg gtgcagcccg ctagcgagcg ataaccgagc gccatctcgc
    13681 ccgtaccgcg cgcatcggca acccactcgg cgacgttgcc cgcttgatcg cgcgtgccgt
    13741 acgggccttc gccccatgga tacgagccgg gtggcgcgat cagcgcgaag ccatcgacgt
    13801 cgtccggatc gcctaacagg tgcagcggat cgccgacgcg atcgatatcg cgcattgcct
    13861 gcgcgcgcgg ctggccatga ttgaaatcgc gcgttcgctc gacgcagagc ttgtcggtct
    13921 ggtctttgat cgcctcgtcg caccacggcc acaccttctc cgcggcacgc gcctcgtctg
    13981 gatcgtcacc gcgtgcagcg cgctcccact cggcctcggt cggcaaccgc ccaccgcgcc
    14041 accggcagta ctcctgcgcc tcgaaccacg tgacgttcac gatcggccag cgatcttgga
    14101 tgtagcgctc gtcgccgccg atgagcggat cgaggctgca gacgcccgcg gtaatgcact
    14161 cgcggaagtc gcggacgctg acttcgttgc ggtcgatggc aaaggcgctg atgaatacgc
    14221 gccgtaacgt catctgcgcg agctcgtcgc gatagccggt gcagagcgtg acgcgtccgt
    14281 tgatcgcgag gatctgcgac tgcggatcga agagcatctc gcactgaccg gcgatcgtct
    14341 cggcatccgc ttcgccgacg cccatccaga aggagcctgc cggcacgaac acttcgcggc
    14401 tcggcgcttg ctcgatccgg atcaccttgc cattgcccgc ggccgcgatc gcgacagcga
    14461 cgctggaggc ggtggcggcg gcgaggatgg cgcggatgcg catcacgggg gaatcctggc
    14521 acgtcagcga gcgagaagcc acgcgaggac ggtgcggacc cggtcacgcg agcccggcgg
    14581 cgccaacgcg aagttggtga ggagcgagtc ggcaacggac gcacgcccgc ggcgctcggc
    14641 ccagcgtacg agtgcatctc tgcgaacatc accgtcgacg tcggcacgcg cgagccggct
    14701 gatcgtctcg tcatcatcga gcgcgggaat cagcgcgcgg cgcacgaacg gtgacggatc
    14761 gtctgcagca tcggcggctt cggccttcag cgttggcgca ccaccgcgcg cgtgcgtctg
    14821 ccaggcggcg gcgcggacgt ccggatcgcg atccgcgagg agcgtgctca ggtcgtgcgt
    14881 cggcgtcacg gctgctgcgt aggcggctcg cacctgcgat gcaggatcat cgtcgacgct
    14941 gacgacgtcg tccggcaacc tggcgaggtg cgcgaccgcg gccgctcgtg ttgcaatgtc
    15001 gcgatccttg gccaggacgc gcagcttggc gcgaacgctt ccgggatctc ggacgctgct
    15061 cgtgcacgcc gcgcgcacgc tcgcatcgcg atcggacatg cccttcacga gccacttctt
    15121 cgcatccgcc ggcgcgaagc tgcccagcgc ctcgcatgcg gccgctctcg tgcgtgcgtg
    15181 cttgctcgcg agccacgtcc cgatctgtgc actgacttcg ggcatcgcga cgaggcc
//
LOCUS       DATLBZ010000260        14394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1028190, whole genome shotgun sequence.
ACCESSION   DATLBZ010000260 DATLBZ010000000
VERSION     DATLBZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14394
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028190"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VMZ53_13685"
     CDS             <1..232
                     /locus_tag="VMZ53_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29557.1"
                     /translation="DADNDDAEEAAGQVAMKEPRRPSQRSFTTSDEGDKTPLPPPTAH
                     DDEDSKPGDAPKFADPPSDADANAKDSKEAQS"
     gene            453..2615
                     /locus_tag="VMZ53_13690"
     CDS             453..2615
                     /locus_tag="VMZ53_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29558.1"
                     /translation="MKRILLAALVAFAVAPNGGAPAQAQISAAIGKPLPSADMPAGQV
                     SVRVVAGSPSAPVSGTEVTLLVNGTPQIARTDSAGRAFFKDLPAGAKLQAKAVDEDKK
                     EIASEEFTAPSDSGVRVMLTTRPWNPGGGAGMTGGEGGAMPNPRQMSGEPRPEDKDPP
                     GSLTVRLSYDDFKDAPPVGVPVALVGYSADDSVKFARVESDKEGRATFTGLDRTGATS
                     YFAMTLLPRGTGVDRLSSTPAVLDPRVGVRLILSGEKRSSTAPNVDDLTKLEKQEVAV
                     PAGKVRIALEGGVEDQEIRLIDAESKTVLGRGRPQLGKPNPQDIQASAQFSAKPDLEA
                     GTLDVLVHGGGGGDDRPLSDVSIKVVAANADGVPAPDAVALADLQTTDNGQARTKIAK
                     LDVPLVATILINGKPLTSKPFDLVKSGGQLVVEAHWDSAGKPEAMFDLVPRPGQVVFA
                     ETSMHGNLYRSLPFQPVTDRGSSATLFIFPRILFTFSITSRLDDEYLAVNGRFELSNN
                     SWVPYSGGPDGVMVPLPKGFTGGLVAEMDQADVAIEPKEGFRIIRPIAPGTKQFHGAF
                     SLPVDDGSVSWSMDLPWGAFNSGMEILQVPGMSVDTPKGADGQVMNVPQGSFFVMPRI
                     QILPKQAMVLTIHGLPQPAKWRVWVPRIIGSLVVLLIIGGVVFAMVRSKSDLAGRGAR
                     RQQLLDALVAMEKAGSTNQARKDELIKELEELWDDGPT"
     gene            2618..3331
                     /locus_tag="VMZ53_13695"
     CDS             2618..3331
                     /locus_tag="VMZ53_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS29559.1"
                     /translation="MAELHRVEIQKIAKRYGSERALAGVTLELRKGSMTALLGHNGAG
                     KTTLLGVLSTLVRPNSGEVRYKTADKDIKGEELRREIGMLAHSSLCYAELSAVENLEL
                     VAGLYGVDGSDASIKAVLDRVGLDPRARGRASRTYSRGMLQRLALARALLTKPSLLLL
                     DEPFTGLDRDGALNLGKQLGELKDAGAIVVVVTHDLEAIAGRTDHLAILRRGALVFED
                     RATSYSYEELKDLYHRHAN"
     gene            3334..4014
                     /locus_tag="VMZ53_13700"
     CDS             3334..4014
                     /locus_tag="VMZ53_13700"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HUS29560.1"
                     /translation="MSLLRQAGRVAWKDLRIELRSKEIVMTMVFFAALLVVIYSFAFP
                     RDDDAVRGAAPGMLWVALAFTGTIALGRAFDRERENDTMRALLLSPIPRLAVFLGKAA
                     SMCLLILAVAVIAVPLLALWLNAPLFQYPGELALVVVLGAIGISVVGSVFAATLLKVR
                     SRDVLLPVILYPLLVPLFVAGTKTTASLVAQHPNLDAAWYWIEFLAVYDAAFLVLSLW
                     IFESLVIE"
     gene            4011..4724
                     /gene="ccsA"
                     /locus_tag="VMZ53_13705"
     CDS             4011..4724
                     /gene="ccsA"
                     /locus_tag="VMZ53_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HUS29561.1"
                     /translation="MKKLAVPILCVLAALGFAASVYLVFMKAPLQYGYTQDGHLNGSS
                     LFWNQKIFYWHVAHAFWLFGAVFVAGVASIIFLWKRDPNWDDIASAAVDVAIAFGAVV
                     LITGSIWAKAAWDVWWNWEPRLTMSLLLWLILVGYALVRKFAGPSADRVAAGMAIFGM
                     VGVPFIYKMVGEDSHPASGKEGVVATLGPGMKGAFWLSVAAFLLWFIALMITRIQSTR
                     CERELRELRERGLDLGVLE"
     gene            4721..5116
                     /locus_tag="VMZ53_13710"
     CDS             4721..5116
                     /locus_tag="VMZ53_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29562.1"
                     /translation="MKKWILIFTLALTGVTFADEPRVPPEAPTAIAPIPGAEARQCCT
                     QAMNADPDFAKSIISTVDKQIDQKTIDAHEAAAKKIQRDDAHVIYAYAAMFVVAALFL
                     AFLWMRQQALMKEIAQLRRDLEAAAKGSK"
     gene            5113..5289
                     /locus_tag="VMZ53_13715"
     CDS             5113..5289
                     /locus_tag="VMZ53_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29563.1"
                     /translation="MTSSHVIFIPGILMVGMFLGFILGTRAARNQFDLQRKREEERAA
                     AKAARAAKKSAKSE"
     gene            complement(5294..6118)
                     /locus_tag="VMZ53_13720"
     CDS             complement(5294..6118)
                     /locus_tag="VMZ53_13720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29564.1"
                     /translation="MSESFGAWQLESLVAVGGLGEVWRARRGDRVAALKRLHTHLARN
                     EEGLRQFSVEQHLTTTLPHHRNLVHAFEAGDVEGRPYVALELIEGEDVRRLVAPPVPQ
                     KTAAPVHVVIPRSRAVGIVLAACDAASHLHAHGYVHGDICPGNLVVDAFGGQANEGSA
                     DRTVLVDLGVSRVIGEAGAVRGTHAYMAPEQVRAEPWTQATDVFAIGIVLWELLAGAR
                     LFHRGPPWLSMAAVVEADVPPLADAALDAIAQAALAKDPAARIQTAAELAQRLRAL"
     gene            6198..8255
                     /locus_tag="VMZ53_13725"
     CDS             6198..8255
                     /locus_tag="VMZ53_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29565.1"
                     /translation="MGERLGRYTVLKHLASGGMADVLLGRTDGIEGFERHVVLKRIRA
                     EHAKDQRFISMFLDEARVAANLHHQNVVQVYDIGESGGEFFFAMEYIHGEDLRRLLSM
                     VSKSKTHMPLGYVVAIVSSAASGLHYAHDRKGNDKKPLNIVHRDVSPSNILIGYDGAV
                     KVVDFGIAKAAMRQVETKSGSLKGKVSYMSPEQCKGEDIDRRSDVYALGVLLYELSTT
                     TRLFKGDNDYLLMDAIVNGKVPLPRVRRPDLPNELSSIIMRALSVDPNRRYQSADELR
                     IALDQFAVQAGLTCSTSALAGYMHKMFGERPEPWLDTAGATVDQAKAIDVEGPTAAGD
                     SKPKVHHSWTELSREQIDAEAETSTGGSENDDAKTRTDGKGNSGRVASRRSSLNNVVA
                     LQDTIAPPITAKETRTSMKMGWEAQGSMPSLPANRARWALAAIPLLALVGVAVWQFSK
                     KNDEQKPAAQPVAATMNPAPSAPTTPTPIVAPAPAAAPVAEEPPALRVAGFEGDKPEE
                     KPPVATPPPAVVKQPVHVAAAKKTPVAPTKVAPPPVAPQKTPTPAVVQTPVAPTPAPT
                     PAPTPPPAATPPPQPAVAAQPAPQIVEAPQMSVLSNATVSAVASDHSRELGKCEGGAE
                     LHGDVAVSFQIDAAGKVVKSQLSASIKNPKVSACILKSVQGWKFPKPPSGAAKGVYSI
                     SYQ"
     gene            8279..9421
                     /locus_tag="VMZ53_13730"
     CDS             8279..9421
                     /locus_tag="VMZ53_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS29566.1"
                     /translation="MDLRLTEEQDALVSTAREFTRKEIIPKAGHWDESGEFPRDLMKK
                     AWETGLMNIEVPAEYGGLGGSCLDNCLVQEEVAFGCAGVNTSMAANSLGATPLLVAGT
                     DEQKKKFLGRLTSEFGFCAYCCSEPDAGSDVAGMRTRVTKHGDEYVLNGQKRWITNGG
                     VAGFYTVFATFDPAMKHKGIACFVVDANTPGVKAGRKENKMGHRASNTTDVIFEDVKL
                     PKNALVGTEDGGFKVAMKTFDRSRPWIAASAAGLIRRCLEESRAYALERKTFGVAIAQ
                     HQAIQFMLAEMAIAYESTRLLTHKAAWLVDQGDLSSIVSSYAKAYGADAAMRCSTDAV
                     QIFGGYGYTKEYPVEKLMRDAKLLQIYEGTSQIQRMVIARNVLGGK"
     gene            complement(9422..10351)
                     /locus_tag="VMZ53_13735"
     CDS             complement(9422..10351)
                     /locus_tag="VMZ53_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HUS29567.1"
                     /translation="MLRGWIPILLLAACGYESDLVACGELLCPAGSTCTANDTCNNPL
                     LPDGDGDGVIDLDDNCVLESNDSQSDVDHDGVGDECDNCPLLPNTEQKNVGDADEVGD
                     GCDPHPTLDGDCLVLVDSFRTPELFPLHWRVFHATGDSSTFTPGDGFVQITPGGTGGA
                     TTAVLEGTTPYTGRFDVQIRAEAALGANGSIGALSAGGAGQGYICGVGRDNVGAAVEL
                     PNQTLQPTINPLLAEPVGDDLLLWLSVPAGTRKDLLCRATYGLALGVASYLDAPLFEG
                     VAGFGVSGDPVKVQAFAMFRFTPGAACAKPIVR"
     gene            10383..11201
                     /locus_tag="VMZ53_13740"
     CDS             10383..11201
                     /locus_tag="VMZ53_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29568.1"
                     /translation="MRAWALLLLGGCGRLAFDPLATDAPKGDAAILPPFVVGQQIASL
                     ASPFADDDPTITPDGLELIFASDRTGNTDLYVSRRASRGDPWSAPTLIAELATVGVEK
                     GPEYSDDGLTLWFEDDGKIAMTTRATRGSAWATPQIALAFTVSLGTPTVCNGDLQMIM
                     RTVDATSANELYTSSRASVGDAWPTPVPLDDIETGQDESGPFLTPDCSRLYFDSVGVF
                     VSDRDPVSGHYGAPVRVIDQALQTCHDISLSDDEHYAVFSKKVSNIEQLWEATR"
     gene            11221..13083
                     /locus_tag="VMZ53_13745"
     CDS             11221..13083
                     /locus_tag="VMZ53_13745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29569.1"
                     /translation="MGVELDSTQQSFPPYGELTQTKAWAAKTPLVHTNVDEATAAVEV
                     SHGSRYTEGAELGRGGMGRVVLARDTRVGRDVAVKVLHPERELEPEERSRFLREAQVQ
                     GQLEHPSIVPVYDIERRPDGTTFFTMRRVLGRTLSAILDDLRTGVPAAKARYTQRELL
                     TAFSTVCLTIDYAHSRGVVHRDIKPANIMLGDFGEVYVLDWGLARLVDDKGESSDKPQ
                     ERLSMPGAFLGTPMYMAPEQMADPDVAPVADVYSLGLILFEILTLERCRDPNALFAPV
                     NAKASVRTPHRDVPPELEAVCVRATEMEPSARYSTPRAMQEAINRYLEGDREHEARRQ
                     LAAKHMDAARIALGKAAGPNADFEKYRGRAITDLGRALALDPGNTEYLALLGELMNAQ
                     TSQTPAAVVQQIEDDNQRVIHAGAKHSATAIVSWWLFLPILLWLGIRDAWQMWLIGVP
                     VTVSALIGLVMVRQKRIGNGIQIVAIFFMLLATMAVSRIYGPFVLLPTLICTYAIVLQ
                     AHPTRMMRIAGAVIATIAITVPALLEWMGALPASYRFEDGAWKIVPQLVDLPKTGTNV
                     FLIAANLAMIIVPCLFIAKLRDELSRAQIRLAVQAWHWQNLGAQLIGRPEATRP"
     gene            13096..13272
                     /locus_tag="VMZ53_13750"
     CDS             13096..13272
                     /locus_tag="VMZ53_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29570.1"
                     /translation="MPECRECKDEVEKLVSVKVGGKARKVCADCADRLREEGEIAEAS
                     EGVIQGMMGYKGRR"
     gene            13307..14053
                     /locus_tag="VMZ53_13755"
     CDS             13307..14053
                     /locus_tag="VMZ53_13755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HUS29571.1"
                     /translation="MQSGVQNIAAGSGPTTIAPPSPPVRGQAPDVFGNIFIGIAGMIG
                     AGKSTLATALGQHLGIDCYYEPVADNEYLADFYKDTARYSFAMQVYLLNRRFQQHQEI
                     IWRGKSAVQDRTIYEDSIFAKMLAQTGLMDERDYRTYVELFRNMSNFMCKPSVIVYLD
                     VSPDSSAERIRMRSRDVESQIPLGYLQALHAGYQEFIANISRTIPVIRIDYERFATAE
                     EMAEVIKREYLDASFLRQVTRFDPATGVSP"
     gene            complement(14100..>14394)
                     /locus_tag="VMZ53_13760"
     CDS             complement(14100..>14394)
                     /locus_tag="VMZ53_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29572.1"
                     /translation="EAVWLREYIRERLKLGVEMSGISCAGNVCSYDGMEYAPNGYLVF
                     KEIDDRWTLIAWTQVYSAALSQEAADANIRSVIANMIKLRDTRCAGEPAGTYY"
BASE COUNT         2573 a         4683 c         4700 g         2438 t
ORIGIN      
        1 ggacgccgac aacgacgatg ccgaagaggc cgccggccag gtcgcgatga aggaaccgag
       61 gcggccttcg cagcgatcgt tcacgacgtc ggacgaagga gacaagacgc cgctgcctcc
      121 gccgacggcg cacgacgacg aagactcgaa gcccggcgac gcgcccaagt tcgccgatcc
      181 gccgtcggac gcggatgcaa acgcgaagga ctcgaaggag gcgcagtcgt gatgcgcgca
      241 ttcgtcatgg agcttcctcg agggacccgg atcgtgaaac acggagccac ggagcacacg
      301 aaggtgagat cggaacgcca acccgatcgc aacgtgcaag ggctcgaggg acgtgttcgc
      361 gacgcgtctc cgacccccac actcctctct gactccgtgt gcttcgtgtc tccgtgttca
      421 agatccggct gggtcctagc ggaggcgtcg tcgtgaagcg catcctattg gcggccctcg
      481 tcgcgtttgc ggtcgcgccg aacggtggcg cacctgcgca ggcgcagatc agcgccgcga
      541 tcggcaagcc gcttccttcg gccgatatgc cggcggggca ggtgagcgtg cgcgtggtcg
      601 ccggcagccc gtccgcgccg gtgagcggca ccgaggtcac gctgctcgtg aacggcacgc
      661 cgcagatcgc gcgcaccgat tcggcaggcc gcgcattttt caaggatctg ccggcgggcg
      721 cgaagctcca agcgaaggcc gtcgacgaag acaagaaaga gatcgcgagc gaggagttca
      781 ctgcgccgag cgacagcggc gtgcgtgtga tgctgacgac gcggccgtgg aatcccggcg
      841 gcggcgcggg catgacgggc ggcgagggcg gcgcgatgcc aaaccctcgg cagatgtcgg
      901 gtgagccgcg tcccgaggat aaagatccgc cgggctcgct gacggtgcgg ctctcgtatg
      961 acgacttcaa agatgcaccg ccggtcggcg tgcccgtcgc gctcgtcggc tactcggccg
     1021 acgacagcgt gaagttcgcg cgcgtcgaga gcgacaaaga aggtcgcgcg acgttcacgg
     1081 gattggaccg cacgggcgcg acgtcgtact tcgcgatgac gctgctgccg cgcggcaccg
     1141 gcgtcgatcg tttgagctcg acgcccgcgg tgctcgatcc gcgcgtcggt gtgcgcctca
     1201 ttctgtccgg agagaagcgc agctctaccg cgccgaacgt cgacgatctg acgaagctcg
     1261 agaagcaaga agtcgcggtc ccagccggca aggttcggat cgcgctcgag ggcggcgtcg
     1321 aggatcagga gatccggctc atcgatgccg agtcgaagac cgtgctcggc cgcggacggc
     1381 cgcagctcgg caagcccaat ccgcaggaca tccaggcgtc ggcgcagttc agcgcgaagc
     1441 cggatctcga agcgggaacg ctcgacgtgc tcgtgcacgg cggtggcggt ggcgatgacc
     1501 gcccgctctc cgacgtgtcg atcaaagtgg tggcggcgaa cgccgacgga gtgcccgcac
     1561 cggacgccgt cgcgctggcg gatctgcaga ccaccgacaa cggacaagcg cgcacgaaga
     1621 tcgcgaagct cgatgtaccg ctcgtcgcga cgatcctcat caatggcaag ccgctcacga
     1681 gcaagccatt cgatctcgtg aagagcggcg gccagttggt cgtcgaagcg cactgggatt
     1741 cggcgggcaa gccggaggcg atgttcgatc tcgtgccgcg acccggtcag gtcgtgttcg
     1801 ccgagacgtc gatgcacggc aatctgtatc gctcgctgcc gtttcagccc gtcaccgatc
     1861 gcggctcgag cgcaacgctg ttcatcttcc cgcgcattct gttcacgttc tcgatcacgt
     1921 cgcgtctcga cgacgaatac ctcgctgtga acggacgctt cgagctgtcg aacaactcct
     1981 gggtgccgta ctccggcgga ccagatggcg tgatggtccc gctgccgaag ggatttaccg
     2041 gtgggctcgt cgccgagatg gatcaggccg acgtcgcgat cgagccgaag gaaggcttcc
     2101 ggatcatccg gcctatcgcg ccgggcacga agcagttcca cggcgcgttc tcgctacccg
     2161 tcgacgacgg cagcgtcagt tggtcgatgg atttgccgtg gggcgcgttc aacagcggca
     2221 tggagatcct gcaggtgccc ggcatgtcgg tcgacacacc gaagggcgcc gacggccagg
     2281 tgatgaatgt gccgcaaggc tcgttcttcg tgatgccgcg cattcaaatc ctgccgaagc
     2341 aggcgatggt gctgacgatc cacggcttgc cgcagccggc gaagtggcgc gtctgggtgc
     2401 ctcgcatcat cggctcgctc gtcgtgctgc tgatcatcgg cggcgtcgtg ttcgccatgg
     2461 ttcgcagcaa gtctgatttg gctggccgcg gagcgcgtcg ccagcagttg ctcgacgcac
     2521 tggtcgcgat ggagaaggcc ggaagcacga accaggcgcg caaggacgag ctgatcaagg
     2581 agctcgaaga gctctgggac gacggcccga cgtaggcatg gcggagctgc atcgcgtcga
     2641 gattcaaaag atcgcgaagc ggtatggcag cgagcgcgcg cttgcgggcg tgacactgga
     2701 gctgcgcaag ggctcgatga ccgcgctcct cggtcacaac ggcgccggta agacgacgct
     2761 cctcggtgtg ctctcgacgc tcgttcgacc caactcgggc gaggtccgtt acaagaccgc
     2821 ggacaaggac atcaagggcg aagagctgcg ccgcgagatc ggcatgctcg cccacagctc
     2881 gctctgctac gcggagctct ccgccgtcga gaacctcgag ctggtcgcag ggctctacgg
     2941 cgtcgacggc agcgatgcgt cgatcaaggc tgtcctcgat cgcgtcggcc tcgacccccg
     3001 cgcccgtggt cgcgcctcgc gtacgtactc gcgcggcatg ctgcagcgcc tcgcgctcgc
     3061 tcgcgcattg ctcacgaagc cgtccctgct cctcctcgac gagccattca cgggcctcga
     3121 tcgcgatggt gcgttgaacc tcggcaaaca actcggcgag ctcaaagacg cgggcgcgat
     3181 cgtcgtcgtc gtgacgcacg atctcgaagc gatcgcgggc cgcaccgatc acctcgcgat
     3241 cttgcgtcgc ggtgcgctcg tcttcgaaga tcgcgccacc tcgtattcat acgaggagct
     3301 caaagacctc tatcatcggc acgccaactg accatgtcgc tccttcgcca agccggccgc
     3361 gtcgcatgga aggacctgcg catcgagctg cgcagcaaag agatcgtgat gacgatggtc
     3421 ttcttcgcgg cgctgctcgt cgtgatctac tcgttcgcgt tcccgcgcga cgacgatgcg
     3481 gttcgcggcg cagcacctgg catgttgtgg gtagcgctgg cgttcacggg cacgatcgcg
     3541 ctcggccgcg ccttcgatcg cgaacgcgag aacgacacga tgcgcgcgct cttgctctcg
     3601 ccgatcccgc ggcttgcggt gttcctcggc aaggccgcgt cgatgtgcct cctcatcctt
     3661 gcagtcgccg tgatcgcggt cccgctgctc gcgctgtggc tcaacgcgcc gctgtttcag
     3721 tatcccggcg agctcgcgct cgtcgtcgtg ctcggtgcga tcggcatctc cgtcgtcggc
     3781 tccgtgttcg cggcgacgct gctcaaagtt cgctctcgtg acgtcctgct gcccgtgatc
     3841 ctctatccgc tgctcgtgcc gctgttcgtc gcaggaacga agacgacggc gtcgctcgtc
     3901 gcgcaacacc cgaacctcga tgccgcttgg tattggatcg agttcctcgc ggtctacgac
     3961 gctgcgttcc tggtactgtc cctttggatt ttcgaatccc tggtgatcga atgaagaagc
     4021 tcgctgttcc gatcctgtgc gtgctcgccg ccctcggctt tgcggcatcg gtctacctcg
     4081 tcttcatgaa ggcgccgctg cagtacggct acacgcagga cggccacctc aacggctcgt
     4141 cgctgttctg gaaccaaaag atcttctact ggcacgtcgc gcacgcgttc tggctgttcg
     4201 gcgcggtctt cgtcgccggc gtcgcatcga tcatcttcct gtggaagcgc gacccgaatt
     4261 gggacgacat cgcaagcgca gccgtcgacg tcgccatcgc ctttggcgcg gtcgtgctca
     4321 tcacgggctc gatctgggcg aaggctgcgt gggacgtgtg gtggaactgg gaaccgcggc
     4381 tgacaatgtc gctgctgctc tggctcatcc tcgtcggcta cgccctcgtc cggaagttcg
     4441 cggggccgag cgcggatcgc gtcgccgcgg gcatggcgat tttcggcatg gtcggcgtgc
     4501 cgttcatcta caagatggtc ggagaggact cgcatcccgc gtcgggcaag gagggcgtcg
     4561 tcgcgacgct cgggcctggc atgaagggcg ccttctggct gtctgtcgcg gcgttcttgt
     4621 tgtggttcat cgcgctgatg atcacgcgga ttcagagcac gcgttgcgag cgcgagcttc
     4681 gtgagctgcg cgagcgcggt ctcgatttgg gagtgctcga atgaagaaat ggatcttgat
     4741 cttcacgctg gctctcaccg gcgtcacgtt tgccgacgag ccgcgcgttc ccccggaggc
     4801 gccgaccgca atcgctccga ttccgggagc agaggcacgc cagtgctgta cgcaggcgat
     4861 gaacgccgac cctgatttcg cgaagtcgat catctcgacg gtcgataagc agatcgatca
     4921 gaagacgatc gacgcgcacg aagcagcggc caagaagatc cagcgcgacg atgcgcacgt
     4981 catctacgcc tacgccgcga tgtttgtagt tgcagcgctc ttcctcgcgt tcttgtggat
     5041 gcggcagcag gcgctcatga aagagatcgc gcagctgcgc cgtgatctcg aggcggccgc
     5101 gaagggcagc aaatgacgtc gagccacgtc atcttcattc ccggcatcct gatggtcggc
     5161 atgttcctcg gcttcatcct cggcacgcgc gcggctcgca atcagttcga cctgcagcgc
     5221 aagcgcgagg aagaacgcgc cgccgcgaag gctgcacgcg ccgcgaagaa gtccgcgaag
     5281 tcagagtaac gagctagagc gctcgcaacc gctgcgcgag ctcggccgcg gtctggattc
     5341 gcgccgccgg atctttcgcg agcgcggcct gcgcgatcgc atcgagcgct gcatccgcga
     5401 gcggcggcac atcggcttcg acgaccgcag ccatcgacag ccacggcggt ccgcgatgaa
     5461 acagccgcgc gcccgcgagc agctcccaga gcacgatgcc gatcgcgaac acgtccgtcg
     5521 cttgcgtcca tggctcggcg cgcacttgtt ccggcgccat atacgcgtgc gtgcctcgca
     5581 ctgcacctgc ctcgccgatc acacgcgaga cgccgagatc gaccagcacc gtgcgatcgg
     5641 ccgagccttc gttcgcttga ccaccgaagg cgtcgaccac gaggttccct gggcagatgt
     5701 caccgtgcac gtagccgtgc gcatgtaggt gcgacgccgc atcgcacgcc gccaagacga
     5761 tcccgactgc tcgactgcgg ggaatcacga cgtgcacggg agcagcggtt ttttgcggca
     5821 ccggtggagc gacgaggcgg cgcacatcct cgccttcgat cagctcgagc gcgacatatg
     5881 gacgcccttc aacatcgcct gcctcgaacg cgtgcacgag gttgcgatgg tgcggcagcg
     5941 tcgtcgtgag gtgctgctcg acggagaatt ggcgaagacc ttcttcgttg cgggcgaggt
     6001 gggtgtgcag gcgcttcagc gccgcgacgc gatcgccacg ccgcgctcgc cacacttcgc
     6061 caaggccacc gaccgcgacg agcgattcga gttgccatgc gccgaagctc tccgacatgt
     6121 aggcgggaga aattatcacg tgagcgtgcg tactcgtcga tcttgatccg atcgaccgcg
     6181 cttcgtacga tcaacgaatg ggtgagcgcc tggggcggta tacggtcctg aagcatttgg
     6241 cgtcaggcgg aatggctgac gtgttgctcg gccgcacgga cggaattgaa ggcttcgagc
     6301 gccacgtggt gctcaaacga attcgcgcgg agcacgcgaa ggatcagcgc ttcatctcga
     6361 tgttcctcga cgaagctcgc gtcgctgcga acctgcacca ccagaacgtc gtgcaggtct
     6421 acgacatcgg cgagtccggc ggcgagttct tcttcgcgat ggaatacatc cacggtgagg
     6481 atctgcgccg cttgctctcg atggtgtcga agagcaagac gcatatgccg ctcggctacg
     6541 tcgtcgcgat cgtgtcgtcg gcggcgtcgg gcctgcacta cgcgcacgat cgcaaaggca
     6601 acgacaagaa gccgctcaac atcgtccatc gcgatgtgtc gccgtcgaac attctcatcg
     6661 gctacgacgg cgcggtgaag gtcgtcgact tcggtatcgc gaaggccgcg atgcgccagg
     6721 tcgagacgaa gtcgggcagc ctcaaaggca aagtcagtta catgtccccc gagcagtgca
     6781 agggcgagga catcgatcgc cgcagcgacg tctacgcgct cggcgtcctg ctctacgagc
     6841 tctcgacgac gacgcgcctg ttcaagggcg acaacgacta tctgctgatg gatgcgatcg
     6901 tgaacggcaa ggtgccgctg ccgcgcgtcc ggcgtccgga ccttccgaac gagctgtcgt
     6961 cgatcatcat gcgcgcgctc tcggtcgatc cgaatcgccg ctatcaaagc gccgatgagc
     7021 tgcgcatcgc gctcgatcaa ttcgccgtcc aagcaggcct gacgtgcagc acgtccgcac
     7081 tcgcgggcta catgcacaag atgttcggcg agcgtcccga gccgtggctc gacaccgccg
     7141 gagcgacggt cgatcaagcg aaggcgatcg acgtcgaagg cccgacggcc gccggtgatt
     7201 cgaagcccaa ggtccatcac agttggaccg agctctcgcg cgaacagatc gatgcggagg
     7261 cggaaacctc gacgggtgga agcgagaatg acgacgcaaa gacgcgcacc gacggcaagg
     7321 gcaacagcgg acgcgtcgct tcgcggcgca gctcgttgaa caacgtggtc gcactgcagg
     7381 acacgatcgc gccgccgatt acggcgaaag aaacgcgcac cagcatgaag atgggctggg
     7441 aagcgcaggg ctcgatgccg agcctgcctg ccaaccgcgc ccgatgggcc ctcgcggcga
     7501 tcccgctgct ggcgctcgtc ggcgttgcag tctggcagtt ctcgaagaag aacgacgagc
     7561 agaagccggc agcgcagccc gtcgccgcga cgatgaatcc agcgccgtcc gctccaacga
     7621 cgccaacgcc gatcgttgcg ccggcgccag ctgctgcgcc cgtagccgaa gaaccacccg
     7681 cgctgcgcgt tgcaggcttc gagggcgaca agccggagga gaagccgcct gtggcaaccc
     7741 cgccgccagc cgtcgtgaag cagcccgtcc acgtcgcagc ggcgaagaag acgccggtcg
     7801 caccgacgaa ggtcgcgccg ccgccagttg cgccgcagaa gacgccgacc cccgccgtcg
     7861 tgcagacgcc ggtcgcgcca acgcccgcgc cgacgccggc tccgacgccg ccgcccgctg
     7921 ccacgccgcc tccgcagccg gctgttgccg cgcagcccgc gccgcagatc gtcgaggctc
     7981 cgcagatgtc ggtgctgtcg aacgcgaccg tctccgcagt ggcgtcggat cactcgcgcg
     8041 agctcggcaa gtgcgaaggc ggcgcggagc tccacggtga cgtcgcggtg tcgttccaga
     8101 tcgacgcggc gggcaaggtc gtcaagagcc agctctccgc gagcatcaag aatccgaaag
     8161 tctctgcctg cattctgaag tcggtgcagg gctggaagtt cccgaagccg ccgtcgggcg
     8221 cagcgaaggg cgtttactcc atctcgtatc agtagcgctg cggtagagtg cggcggtcat
     8281 ggacctccgc cttaccgaag agcaagacgc cctcgtctcc accgcgcgcg aattcacgcg
     8341 caaggaaatc atccccaaag cggggcactg ggacgagtcc ggcgagtttc cgcgcgacct
     8401 gatgaagaag gcctgggaga cgggcctgat gaacatcgag gttcctgccg agtacggcgg
     8461 tctcggtggc agctgcctcg ataactgcct cgtgcaagaa gaggtcgcgt tcgggtgtgc
     8521 gggcgtgaac acgtcgatgg ctgcaaactc gctcggcgcg acgccgctcc tcgttgctgg
     8581 caccgacgag cagaagaaga aattcctcgg tcgcctcacg agcgagttcg gcttctgcgc
     8641 gtattgctgc agcgagcccg acgccggcag cgacgtagct ggcatgcgca cgcgcgtgac
     8701 gaagcacggc gacgagtacg tgctgaacgg ccagaagcgt tggatcacga acggtggtgt
     8761 cgccggtttc tatacggtgt tcgcgacgtt cgatccggcg atgaagcaca agggcatcgc
     8821 gtgcttcgtc gtcgacgcga acacgccggg cgtgaaggcc ggccgcaaag agaacaagat
     8881 gggccaccgt gcgtcgaaca cgacggacgt gatcttcgag gacgtgaagc tccctaagaa
     8941 cgcgctcgtc ggcacggaag acggcggctt caaagtcgcg atgaagacgt tcgatcgctc
     9001 gcgtccgtgg atcgcggcga gtgctgcggg cctcatccgt cgctgcctcg aagagagccg
     9061 cgcgtacgcg ctcgagcgca agacgttcgg cgtggcgatc gcgcagcatc aagcgatcca
     9121 attcatgctc gccgagatgg cgatcgcata cgagtcgacg cgcctgctca cgcacaaagc
     9181 ggcatggctc gtcgatcagg gcgacctctc gagcatcgtg tcgagctacg ccaaggcgta
     9241 cggcgccgac gcggcgatgc gctgctcgac ggacgccgtc cagatcttcg gcggctacgg
     9301 ctacacgaag gagtatcccg tcgagaagct gatgcgcgat gcgaagctgc tccagatcta
     9361 cgagggcacc tcgcagatcc agcgcatggt catcgcgcgc aacgtgctcg gcggaaagta
     9421 gctaccgcac gatcggcttg gcgcacgctg cgcctggcgt gaagcgaaac atcgcgaacg
     9481 cttggacctt caccggatcg ccgctgacgc cgaatccggc gacaccctcg aacaacggag
     9541 cgtcgagata cgaggcgacg ccgagagcga ggccgtacgt cgcacgacac agcaggtcct
     9601 tgcgcgtgcc ggcgggcaca ctcagccaca gcagcaagtc atcgccgacg ggctctgcca
     9661 gcagcggatt gatcgtcggc tgcagagtct gattggggag ctcgaccgca gcacctacgt
     9721 tgtcgcggcc gacaccacag atatagccct ggccggcacc tccggcggac agcgcaccga
     9781 tcgacccgtt cgcaccgagt gccgcctcgg cgcggatctg gacgtcgaag cgtcccgtgt
     9841 acggcgttgt tccttcgagc actgccgttg tcgcgccgcc tgttccgccc ggcgtgatct
     9901 ggacgaatcc atcgcccggc gtgaacgtcg acgaatcgcc ggtagcgtgg aagacgcgcc
     9961 agtgcagtgg gaagagctcg ggcgtgcgaa acgaatcgac gaggacgagg cagtcgccat
    10021 cgagcgtcgg atgcggatcg caaccatcgc cgacctcatc ggcatcgccg acgttcttct
    10081 gctcggtgtt cggaagcaac ggacagttgt cgcattcatc accgacgccg tcgtgatcga
    10141 catcgctctg gctgtcgttc gactcgagca cgcagttgtc gtcgagatcg atcacgccgt
    10201 cgccatcacc gtcgggcaag agcgggttgt tgcacgtgtc gttcgccgtg caggtgctcc
    10261 cagcgggaca cagcaactct ccgcacgcaa cgagatcgct ctcgtagccg cacgcagcga
    10321 gcagcaatat cgggatccac ccgcgcagca ctccgctcat tgtactcgcg tatgctcggt
    10381 acatgcgcgc gtgggcgctc ctgctgctag gcggctgcgg ccggctcgca ttcgatcctc
    10441 tggcgacgga tgcgccaaag ggtgatgccg cgatactccc gccgttcgtt gtcggtcagc
    10501 agatcgcgtc gctggcgagc ccattcgccg atgacgatcc gacgatcacg ccggatgggc
    10561 tcgagctgat cttcgcgagc gatcgcaccg gcaatacgga tctctacgtg tcgcgtcgag
    10621 catcgcgcgg cgatccttgg tccgcgccga cgctcatcgc cgagctcgcg accgttggtg
    10681 tcgagaaggg tcccgagtat tccgacgacg gcttgacgct gtggttcgag gacgacggaa
    10741 agatcgcgat gacgacgcga gccacgcgcg gatcagcatg ggccacgccg cagatcgcgc
    10801 tggcgttcac cgtgagcctc ggaacaccga ccgtctgcaa tggcgacttg cagatgatca
    10861 tgcgcactgt cgatgccacg agcgcaaacg agctgtacac gagctcgcgc gcgtccgtcg
    10921 gtgatgcgtg gcccaccccg gttccgctcg acgacatcga aaccgggcag gacgaatcgg
    10981 gaccgtttct cacaccggat tgctcgcgac tgtatttcga tagcgtcggc gtcttcgtaa
    11041 gtgaccgcga tcccgtatcc ggacactacg gcgcgcccgt gcgtgtgatc gatcaggcgc
    11101 tgcagacgtg ccatgacatc tctctctccg acgacgaaca ctacgcagtg ttcagcaaga
    11161 aggtctcgaa catcgagcag ctctgggagg cgacgcgatg aacggcggta tgctcgccgc
    11221 atgggcgtag agctcgattc gacgcagcag agcttccctc cgtacggcga gctgacccag
    11281 acaaaggcgt gggctgcgaa gacaccgctc gttcacacga acgtcgacga agcgacagca
    11341 gcggtcgagg tctcgcatgg ctcgcgttac accgaaggcg cggagctcgg acgtggaggc
    11401 atgggccgcg tcgtgctcgc tcgcgatacg cgcgtcggcc gcgatgtcgc cgtcaaggtg
    11461 ctgcatcccg agcgcgagct cgaaccggag gagcgttcgc ggttcttgcg cgaggcgcag
    11521 gtccaggggc agctcgagca tccatcgatc gttcccgtct acgatatcga gcgccgcccc
    11581 gacggcacga cgttcttcac gatgcggcgc gtgctcggtc ggacgctgtc cgcgatcctc
    11641 gacgatctgc gcacgggcgt acctgcggcg aaggcgcgct acacgcagcg cgagctgctc
    11701 actgcgtttt cgacggtgtg cctgacgatc gattacgcac acagccgcgg cgtcgtgcat
    11761 cgtgacatca agccagcgaa catcatgctc ggcgacttcg gtgaggtgta cgtgctcgac
    11821 tggggccttg cgcgtctcgt cgacgacaag ggcgaatcga gcgacaagcc gcaggagcgc
    11881 ctctcgatgc cgggcgcgtt cctcggcacg ccgatgtaca tggcacccga gcagatggcc
    11941 gacccggacg ttgcgcccgt cgcagatgtc tactcgctcg gcctgatcct gttcgagatc
    12001 ctcacgctcg agcgctgtcg tgatcctaac gcgctgtttg cgccggtgaa cgcgaaggcg
    12061 agtgtgcgga cgccgcatcg cgatgtgccg cccgagctcg aggccgtctg cgttcgcgcg
    12121 accgagatgg agccatccgc tcgctattca acgccgcgcg cgatgcagga ggcgatcaac
    12181 cgctatctcg agggcgatcg cgagcacgaa gcgcgtcgtc agctcgcggc gaagcacatg
    12241 gatgctgcac gcatcgcgct cggcaaagcc gccggcccga atgctgactt cgagaaatac
    12301 cgcggccgcg cgatcaccga tctcgggcgc gcgctggccc tcgatccagg caacaccgag
    12361 tacctcgcgc tgctcggcga actgatgaac gcgcagacgt cacaaacgcc cgccgccgtc
    12421 gtgcagcaga tcgaggatga caaccagcgg gtcattcatg cgggtgcgaa gcactctgcg
    12481 acggcgatcg tctcgtggtg gttgttcctg ccgatcctgc tgtggctcgg catccgcgat
    12541 gcgtggcaga tgtggctcat cggcgttccg gtcacggtgt ccgcactgat cggcctcgtg
    12601 atggtgcgcc agaagcgaat cggcaacggc atccagatcg tcgctatctt cttcatgctg
    12661 ctcgcgacga tggccgtcag tcgcatctac ggcccgttcg tgctgctgcc gacgctgatc
    12721 tgcacgtacg cgatcgtgtt gcaggcgcac ccgacgcgga tgatgcgcat cgcaggagcg
    12781 gtcatcgcga cgatcgcgat cacagtgccc gcgctcctcg aatggatggg cgcgctgccg
    12841 gcgtcgtatc gcttcgagga cggcgcgtgg aaaatcgtgc cgcagctcgt tgatctgccg
    12901 aagaccggca cgaacgtgtt tctcatcgcg gccaacctcg cgatgatcat cgtgccgtgc
    12961 ctgttcatcg cgaagcttcg cgacgagctg agccgcgcgc agatacgtct cgccgtgcag
    13021 gcctggcact ggcagaacct tggcgcgcag ctcatcggcc ggcccgaagc cacgcgcccg
    13081 tagtacgatc ggcccatgcc ggaatgccgc gagtgcaagg acgaagtcga gaagctcgtc
    13141 agtgtcaaag tcggaggcaa agcccgcaaa gtctgtgcgg actgcgccga tcgcctgcgc
    13201 gaagaaggcg aaatcgctga agctagtgag ggcgtgatcc agggaatgat gggttacaaa
    13261 ggccgtcgct gagctgatgt cataggcctc tgcgaggctg ccgcccatgc agagtggcgt
    13321 gcagaacatc gcggctggtt cgggtccaac gacgatcgcc ccgccgtcgc ctccggttcg
    13381 tggccaggcg ccggatgtgt tcggcaacat cttcatcggc atcgccggaa tgatcggtgc
    13441 ggggaagtcg acgctcgcca cggcgctcgg tcagcacctc ggcatcgact gctattacga
    13501 gcccgtcgcc gacaacgagt acctcgcgga cttctacaaa gacaccgcgc gctactcgtt
    13561 cgcgatgcag gtctacctct tgaaccgccg cttccagcag caccaagaga tcatctggcg
    13621 cggcaagagc gcggtgcagg accgcaccat ctacgaagac tcgatcttcg ccaagatgct
    13681 cgcgcagacc ggcctgatgg acgagcgcga ctaccgcacg tacgtcgagc tgttccgcaa
    13741 catgagcaac ttcatgtgca agccgtcggt gatcgtctac ctcgacgtat cgccggattc
    13801 gtcggcggag cgcatccgca tgcggtctcg cgatgtcgaa tcgcaaatcc cgctcggcta
    13861 cctgcaggcg ctgcacgccg gctaccagga attcatcgcc aacatctcgc ggacgattcc
    13921 ggtgatccgc atcgactacg agcggttcgc gaccgccgaa gagatggccg aagtcatcaa
    13981 gcgcgagtac ctcgacgcgt cgttcctccg ccaagtcacg cgcttcgatc cggcgaccgg
    14041 cgtctcgccc taacgaacga ctccgccggc gtagaaggcg cgatcgacgg cgccggccgt
    14101 cagtagtacg tgcccgcggg ctcccctgcg caacgcgtgt cgcgcagctt gatcatgttc
    14161 gcgatcacgc tgcggatgtt cgcatcggcg gcttcctgcg agagcgcggc cgagtagacc
    14221 tgcgtccaag caatgagcgt ccaccggtcg tcgatctcct tgaagacgag atagccgttc
    14281 ggcgcgtact ccatgccgtc gtacgagcac acgttgccgg cgcacgaaat gccgctcatc
    14341 tcgacaccga gcttcaggcg ttcgcgaatg tactcgcgca gccagacggc ctcg
//
LOCUS       DATLBZ010000261         3112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1030123, whole genome shotgun sequence.
ACCESSION   DATLBZ010000261 DATLBZ010000000
VERSION     DATLBZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030123"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..990)
                     /gene="gshB"
                     /locus_tag="VMZ53_13765"
     CDS             complement(25..990)
                     /gene="gshB"
                     /locus_tag="VMZ53_13765"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HUS29573.1"
                     /translation="MDVLFIVDPLDKLGLAGDTSYALMLEAAQRGWHVWTCQLEHLGL
                     EHDEPITTAVPTSVVDAATPADAFKTDKPQLKRLGDFDIVLMRKDPPVDVRYLHATWI
                     LEHARGKTLLVNDPRGLRELNEHLAVLRFPHLTPPTIVTRSIDRLRAFQREQGGSIVV
                     KPVDGYGGLGIFVVREGDMNASSIMESSMAIDQPGARWTLAQKYLPEAAAGDKRIILA
                     DGEPVGAVLRVPAEAEARGNLHVGGRAVKTTLDDRDKEIIATIAPFLRSHGQILVGLD
                     VIGGMLTEINITSPTGVRHVSRLDNRNVAAPILDCFERLARAQRS"
     gene            1039..1818
                     /locus_tag="VMZ53_13770"
     CDS             1039..1818
                     /locus_tag="VMZ53_13770"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29574.1"
                     /translation="MRRSWLLVLAVGCGRLQFDARDDAMAGSNGDAPRADAVGDGVAA
                     TTCGTFGSRACDGFEQGAIDPMWTLDTSAGSITRDTTRAFRGSASVHVHIDQITVTTT
                     NPRATLFGPAGLNATVTGMIYFRVWMYVASPLATNLFNQMINAANMSGQGMSLGTRNG
                     VFANNNYTDVNYSESATPFPLDRWMCLQYEMPSNTTGTARMFVDGAELTDAALVKTTP
                     QPPPTHVYLGLEWVGNPSSMPAADAWLDEIIVDTNPTTCAQ"
     gene            complement(1825..>3112)
                     /locus_tag="VMZ53_13775"
     CDS             complement(1825..>3112)
                     /locus_tag="VMZ53_13775"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS29575.1"
                     /translation="DVDVLVEAINLGQVYRYITKPWDAKEVRGVLQYAIERFHLQREN
                     KKLEAQLAEYTGYLNQQLHGEFDFGNIIGESPALREVLSKVEQVAPTVSTVLLRGETG
                     TGKELVAHAIHINSPREEKPFVRVNCAALAPGILESELFGHEKGSFTGAMERRRGRFE
                     LADGGTLFLDEVGDLPMEVQIKLLRTLQERELERVGGNETIKVDVRVVSATNRNLEKM
                     IEEGEFREDLYYRLNVFPINLPPLRDRPEDLPVLLNHFIAKFARQMGVPVQPASPEAV
                     AKLREYNWPGNVRELENIVERAMILARGQPLGAAHLDFGRRSSAPNQSGAVAAPASAA
                     PSAPVAVPTAAAEDGKSLAERLLDSERKEIVAAVEKSRGNIASAARQLGINRSTLYYR
                     LRKHGLEHLLPTKVQVGGEEVPPTGGGGEPPAGSTP"
BASE COUNT          516 a         1015 c         1039 g          542 t
ORIGIN      
        1 gtctgcgccg tcagcccgta agcgctagct gcgttgcgcg cgagcgaggc gttcgaagca
       61 gtcgaggatc ggcgcggcga cgttgcgatt gtcgaggcga gagacgtgcc ggacgcccgt
      121 cggcgacgtg atgttgattt cagtgagcat gccgccgatc acgtcgaggc cgacgaggat
      181 ctggccgtgc gagcgcaaga acggcgcgat cgtcgcgatg atttctttgt cgcgatcgtc
      241 gagcgtggtc ttcacggcac gaccaccgac gtgcaagttg ccgcgcgctt cggcttcggc
      301 gggaacgcgc aggacggcac cgacgggctc gccatcggcg aggatgatgc gcttgtcgcc
      361 tgccgcggct tcgggcaagt acttctgcgc gagggtccaa cgcgcgccgg gctgatcgat
      421 ggccatcgac gattccatga tggagctcgc gttcatgtcg ccttcgcgca cgacgaagat
      481 gccgaggccg ccgtaaccgt cgaccggctt gaccacgatc gagccacctt gctcgcgctg
      541 gaatgcgcgc agccgatcga tcgatcgtgt gacgatcgtc ggcggcgtga ggtgcgggaa
      601 gcggaggacc gcgaggtgct cgttgagctc gcgcaggccg cgcggatcgt tgacgagcag
      661 cgtcttgccg cgcgcgtgct cgaggatcca cgtcgcgtgg aggtagcgca cgtcgaccgg
      721 cggatccttg cgcatcagca cgatgtcgaa gtcgcccaga cgcttgagtt gaggcttatc
      781 ggtcttgaat gcatcggcgg gggtcgccgc gtcgacgacg ctcgtcggca ccgccgtcgt
      841 gatcggctcg tcgtgctcga ggcccaggtg ctcgagctga cacgtccaca catgccagcc
      901 gcgctgcgca gcttcgagca tcaacgcgta cgacgtgtcg cccgcaagtc ccagcttgtc
      961 gagcggatcg acgatgaaga gtacgtccac ccgcagactc tagcaccgca atgaatcatc
     1021 gtgacgtacg ctagaccgat gcggcggtcg tggctcctcg tcctcgcagt tggctgcggc
     1081 cggctgcagt tcgacgcacg cgacgacgcg atggccggca gcaacggcga cgcaccgcgc
     1141 gcagacgctg tgggcgatgg agtcgcggca acgacgtgcg gcacgtttgg cagccgcgcg
     1201 tgcgatggct tcgagcaagg cgcgatcgat ccgatgtgga cgctcgacac gtcggcagga
     1261 tcgatcacac gcgatacgac ccgcgccttc cgtggcagcg cgtcggtgca cgtgcacatc
     1321 gaccagatca cggtcacgac cacgaaccct cgcgcgactc tgttcggtcc ggccgggttg
     1381 aacgcgaccg tcaccggcat gatctacttc cgcgtctgga tgtacgtcgc ctctccgctc
     1441 gcgacgaacc tgttcaatca gatgatcaac gcggcgaaca tgagtggcca gggcatgtcg
     1501 ctaggcacgc gcaacggcgt gttcgcgaac aacaactaca cggacgtcaa ctacagcgaa
     1561 tccgctacgc cgtttccgct cgatcgctgg atgtgcctgc aatacgagat gccgagcaac
     1621 acgaccggca ccgcccgcat gttcgtcgac ggcgccgagc tcacggatgc agcgctcgtg
     1681 aagacgacgc cgcagcctcc gccgactcac gtctatctcg gcctcgagtg ggtcggtaat
     1741 ccgagctcga tgccggcggc ggacgcgtgg ctcgacgaga tcatcgtcga taccaacccg
     1801 actacgtgcg cgcagtgagc gcgatcaggg cgtcgaaccc gcgggaggct cgccaccccc
     1861 accagttggc ggaacttctt cgccgccgac ctgcaccttc gtcggcagca agtgctcgag
     1921 accgtgcttg cgaaggcggt agtagagcgt cgagcgattg atgccgagct ggcgcgcagc
     1981 gctcgcgatg ttgccgcgcg atttctcgac ggcggcgacg atctctttgc gctcgctatc
     2041 gagcaggcgc tccgcgaggg acttgccgtc ttcggccgca gcagtcggga ccgcgaccgg
     2101 cgcagacgga gcggcagagg ccggcgcagc aaccgcaccc gattgattcg gcgccgaaga
     2161 ccgtcgaccg aagtccagat gcgcagcgcc gagcggctgg cccctcgcga ggatcatcgc
     2221 gcgctcgacg atgttctcga gctcgcgcac gttgccgggc cagttgtact cgcgcagctt
     2281 cgcaacggcc tcgggcgacg cgggctgcac ggggacgccc atctggcgcg cgaacttcgc
     2341 gatgaagtga ttgagcagca ccggcaagtc ttcgggccga tcgcgcagtg gcggcagatt
     2401 gatcgggaag acgttgagcc ggtagtagag gtcctcgcgg aactcgcctt cctcgatcat
     2461 cttctcgagg ttgcggttcg tcgcgctgac gacgcgcacg tcgaccttga tcgtctcgtt
     2521 gccaccaacg cgctcgagct cgcgctcttg cagcgtgcgc agcagcttga tctggacttc
     2581 catcggcaga tcgccgacct cgtcgaggaa gagtgtcccg ccatcggcga gctcgaagcg
     2641 gccgcgccgg cgctccatcg cacccgtgaa gctgcccttc tcgtgaccga acagctccga
     2701 ctcgaggatg cccggcgcga gcgcggcgca gttcacgcgc acgaatggct tctcttcgcg
     2761 cggcgagttg atgtggatcg cgtgtgcgac gagctctttg ccggtgcccg tctcgccgcg
     2821 cagcaggacc gtcgagactg tcggtgcgac ttgctcgacc ttcgacagga cttcgcgcag
     2881 cgccgggctc tcgccgatga tgttgccgaa atcgaactcg ccgtgcagct gctggttcag
     2941 gtagccggtg tactcggcca gctgcgcctc gagcttcttg ttctcgcgct gcaggtgaaa
     3001 gcgctcgatc gcgtactgga ggacgccgcg cacttccttc gcgtcccacg gcttggtgat
     3061 gtagcggtag acctgaccca ggttgatcgc ctcgacgagc acgtcgacgt cg
//
LOCUS       DATLBZ010000262        18682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3129992, whole genome shotgun sequence.
ACCESSION   DATLBZ010000262 DATLBZ010000000
VERSION     DATLBZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18682
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129992"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VMZ53_13780"
     CDS             complement(<1..1631)
                     /locus_tag="VMZ53_13780"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase/hydrolase"
                     /protein_id="HUS29576.1"
                     /translation="MNARPIVIACTLVACRSAARAPVDPHSFAEPDRVSVRALTLELT
                     VDFDKKVLAGTAKLELRRVDRSAQLVLDDEGLVIEGVTDCAGKKLGWHVGKHVNIGAP
                     LRIDLGAATECVELTYRTAPDAGALLWVEPSGTAGGVKPMLFTESQAIRARTWIPLQD
                     SPSVRFSYKATIHAPAGMRALMSGENAAEPSGDAWHVEQKQPIPSYLMALAVGDFVFR
                     ATGPRSGVYAEPAIADAAAHEFGEVEQMMAAAEKLYGPYRWGRYDMLVLPPSFPYGGM
                     ENPNLTFLTPTVITGDRALVGLIAHELAHSWSGNLVTNKTWNDVWLNEGITTYVEHRI
                     MEELRGVEYSELLWSIASRDIDETVAEAGSALQTTSLAHAYGRDIAPDDFPADLAYDK
                     GALLMRTLELTYGRATFDAFLRGWFDGHAFSSVDSRMFVAEAKRVLGTKVDLDAWLYK
                     GGIPPGAAPSSSTIATRLAAAANAFATTGTLPDASAWATMEWVVFLRALPKDIKAPQL
                     DALDAAFHLTSTTNNEIAMHWLPLLVNADDHAGAAA"
     gene            1666..2448
                     /locus_tag="VMZ53_13785"
     CDS             1666..2448
                     /locus_tag="VMZ53_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS29577.1"
                     /translation="MRTLVLLTVALLATSCVKKSTYQKAVEENKRLKGELADTQKKLA
                     DTEQTLSDKIKELQAQLGILDETTKNKEAELGKVRTEKEATMAELAELRRQKEAAEKR
                     IAAYKALQDKFRALTDTGKLQVVFRNGQMTLKLPSGILFPSGSDKLSKDGETALADVA
                     KILMEFKERRFIVAGHTDNQPIKTAQFPNNWYLSTARANSVVQFMIKQGFPAKSLAAS
                     GYGEFDPVAANDTDKNRELNRRIEIILVPNLEELPSLTGNQP"
     gene            2508..3077
                     /locus_tag="VMZ53_13790"
     CDS             2508..3077
                     /locus_tag="VMZ53_13790"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29578.1"
                     /translation="MRTLLLAALLFAAACKDDKKPSSSEGATPTERSGDHSAAVSSAK
                     HPALPHDGDDVGSGARPRLPGVDDQRPDWNDPAIRAEWEEKREERRKRMEAELDTNKD
                     GKVSPEEHAQRVKPMLERMDQNNDGKLTPDEMAESERRMGFDDPAALDTDKSGDISLM
                     ELDTAVTARREKMRTKWRGRTGPGMGSDE"
     gene            complement(3081..3971)
                     /locus_tag="VMZ53_13795"
     CDS             complement(3081..3971)
                     /locus_tag="VMZ53_13795"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HUS29579.1"
                     /translation="MKLACAALALIGCVDVADQGGPWEDASSVNGVSIGPAPALRATP
                     GCTLRIASWNVHKLPDANELATALLATQEISRADVVLMQESSGYAAEPMPRTAQLAEA
                     LAMTWAHQPIRDLGDGGMQANAIMSRYPLDRVMVKRLPYIEQPYHAQPRGAIAADIIV
                     GDKRVRVVSVHLDVRISISDRIRQLDPAVSELDEAAVIGGDFNTAPWQWIDGIVPLTS
                     SEAVLGMKQAAALDDYMASRRFDGNVSPNTVTFPIPGFPMRLDNLYSRGVAVTAAGVE
                     QSNGSDHYPLWVDIDLCAAP"
     gene            complement(3968..4459)
                     /locus_tag="VMZ53_13800"
     CDS             complement(3968..4459)
                     /locus_tag="VMZ53_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29580.1"
                     /translation="MRWIAATLLLSTPTAALAEDGITETADAQPSKHLVYVEALGKGG
                     LYGIGYEHGITSRLALGAAGSFAVVSDQQVLTFAPYVHGTIVAGRRHAFFTEVGAVLA
                     HSHLPSPVEGWDGMSDSGGGGFASLGWEYRRNHVVLRASGSVVAGEGGIGPMFGFAVG
                     VRP"
     gene            complement(4482..5882)
                     /locus_tag="VMZ53_13805"
     CDS             complement(4482..5882)
                     /locus_tag="VMZ53_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HUS29581.1"
                     /translation="MFPRLLVGLTLVASVWPGRTASAQPDSDQVYQPAVTEPEDLPVK
                     QKSYLVPTLEVVLGNAAIFTGAYLAGMDWAHVSVDTTKEHLSGGWAFDDDAYSTNQLG
                     HPFTGSLAFSAARSTGHEFWVAGAYSFAGSLLWELVENEIPSTNDMITTPIGGMFIGE
                     AMHRFSRALLYRGYGKPTWIRKTAASVIDPVGAINRAAWGDAWAKTIPPALNAHFAVG
                     WQQPTHLLGAEHGGDGQLHVEAYVEHGVMGDRAFQPRRPLDHFTLQTAIDSSTDELEA
                     LLYVRGMLVGSGVRGDQVRGMYGLFSGYDYNSNHFVRASMLGIGPGGMVEARLGDRGY
                     VAGTGAAYLVPYGAAGGVNEEEGPMNDHNDGPGMAQLLELQGGQRGLWNVRSTTRLYE
                     IRGRLVGDDANEFVATSTLGARVQIKTHQAIGVEGTYSYRRADYADPLMNASTPDTTM
                     DVRAFYAITTDEILGR"
     gene            complement(5972..7120)
                     /locus_tag="VMZ53_13810"
     CDS             complement(5972..7120)
                     /locus_tag="VMZ53_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29582.1"
                     /translation="MRTALLLVCASGCIISPPEGATQSWPPSLVTVQAVTSGDLDGNG
                     STDIVVYGTGGENRAGLYLLTGGQDLGGGQSKPVRSFSTFVPAELAIPTAALQLGGAA
                     PSIYVATAEDGPGDLSNGNIEITQYSNILGEKARETTAVPAGNATLWLHAINFPGAML
                     HIAVSNGGTINHASANLGDVKPIPAASGPTWDLAQLATSYPSGMDQIAVVATPMQIQR
                     ASVPGPSGGMFAWTVVRNGPAWVGQTSIDLDGDGREEILGLDLANKQVCVVDPGASTV
                     PVTPVCLAIPMLPGASEVTLLAGANITMTPGPDILIVQASGSDTQFTLVEDYTYTPGT
                     LISTTIHPLQLAGPAHGRTVIANSGPGTPNSVLVFGTDGAVGCVLGPC"
     gene            complement(7120..8724)
                     /locus_tag="VMZ53_13815"
     CDS             complement(7120..8724)
                     /locus_tag="VMZ53_13815"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29583.1"
                     /translation="MIHRICPTCDKDVQADVTSCPTCNSKLVEIRDEADQVVGTVIDQ
                     RFEVRDKIGQGGMGSVYRAWQRSVGREVAIKLIDRSFSRDPMGVRRFLREARLASQLS
                     QPNTVSVFDFGQADDGRLFIAMELIRGRTLYQVLQAEGAFPIERVKRIGIQICDALEA
                     AHSLGIVHRDLKLENAIVLDHPPGRDLLKVLDFGLAKNIRDPGSRATESGIVVGTPRY
                     MSPEAAMNGTSLPAGDVYALGVILGELATGKPLWEGDNLPQLIGQKMDNAAALARIPA
                     PIRSTIKAMLEPEPDRRPTAAQARAMLSTSDEPNAGDTINEEPNASATVNLRGSSDQQ
                     KSVVGKSDPYAATRKLETPPPGSAPAAPVAARSPTATPAPADSVAAMVDEVKRPARRI
                     ALLAIAAALAIVVVAAIMFMKTGNRSPQVSTAPRDASVQTAVATPDAAQSTVVVDAAV
                     VEAKTAKVMIDTHPSGASITHKGTTYKTPFILELPADSAKVELTASLSGYQTAKLVVG
                     PSDTTKKLVLKKKKAASRDSGEQVPF"
     gene            complement(8751..9803)
                     /locus_tag="VMZ53_13820"
     CDS             complement(8751..9803)
                     /locus_tag="VMZ53_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29584.1"
                     /translation="MTLAAAAIAIALGTSIGSADERWIIEPGPEAADAQKLGAADVER
                     AQALAKKKQFGEAAAVLEAVARKWPAAVHDCNLALAYLRSGELTKAQLVWDLAALRNG
                     VRPKWCTGEVSTQLSDALRKGGYVPTTIDVAPSDAIVEVNGIAMRGMRTVWLKPGVVM
                     FSASAQGRLPKAAQASIAAPVSRVAIVLEEPKVEPPPAPDAGVPIVTPSVTPDAAVVA
                     TPIEPVVAPDAGVDNSFVRVNGRPVGAKKIALVTMLSSGLSAALLGGLAYKTKLDANG
                     LFPTDPAFEEQKQVFDNFAIATTICAGVSVVSAGFYIYFLVAGDTPVSKPGKIQVGGG
                     PGDIGISFSGTFGETR"
     gene            complement(9870..10649)
                     /locus_tag="VMZ53_13825"
     CDS             complement(9870..10649)
                     /locus_tag="VMZ53_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29585.1"
                     /translation="MRDRIETSIMTSLSELRDIEKQRIADERNAAERARNAEIEAKRA
                     AEQAKVDAEEARKRAEREEIIKIQLAQAEREKQARLRVEAAEAAERARLAAELEAQRM
                     AAEVELRREEVARKRPTWMVAVTAVAFVASIGLGVFAYNRAQDTAKAEQAARAAQLRQ
                     EQARQDAAEAQRGLDKLKLESDERAKRVAALELELQGKIDAAKAEEIKKKLADEKKAE
                     DAAKHEAWLLEQKRLSDERNRPVDVSNCVGQVVCKDTGKKK"
     gene            complement(10712..10942)
                     /locus_tag="VMZ53_13830"
     CDS             complement(10712..10942)
                     /locus_tag="VMZ53_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29586.1"
                     /translation="MLAVRTPIAGIFIAVLVLVGQVAVMRAYVEASRPAPAKEHRLHA
                     HHSKVHVPPPKFGHRRGCADGVRISGPDDITR"
     gene            complement(11059..11838)
                     /locus_tag="VMZ53_13835"
     CDS             complement(11059..11838)
                     /locus_tag="VMZ53_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29587.1"
                     /translation="MAQGEKRENSVLFSLRELRQIEENRVQEEASAVKTAEEARIRAR
                     EEAERRKREEEEAKIQAERDHQRQIEEARVNAEREARMRVEAAEAAERQRHQAALEQS
                     RLQQEMELRRAEVAKKRPTWMLAVTGIALVGAIGLIFFAVNRMRESEASDVRRKKAEA
                     EAAQAVKDANDAKDKLDRIMKDLDDLDTKVGAAVSAVADAQNDADRASAKARLVALQR
                     EQFEMKQRAAAARDAAEKAERRKGVKVSKECLDNPLAKGCS"
     gene            11973..12686
                     /locus_tag="VMZ53_13840"
     CDS             11973..12686
                     /locus_tag="VMZ53_13840"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HUS29588.1"
                     /translation="MGFVDLHSHVLYGLDDGAPDETTALAMLDGLTTLGISEQCLTPH
                     QKASQYLPAWDRIEATMAKLEQVRKPHHPTLRLAAENMWDDVFYKRVVEDAIPGYRNT
                     VAFLVELPPPVMPPGIIEQMFKWRMAGKVPVLAHPERYHALWDNDDLAQKLRTHCAFL
                     IDLGALGGFHGKREMKAARHLCEKGLVTAVATDAHQLGDLQQAVEGLRWVEKKLGAAA
                     VTRLFDHAPRAILAGELPD"
     gene            12688..13740
                     /locus_tag="VMZ53_13845"
     CDS             12688..13740
                     /locus_tag="VMZ53_13845"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HUS29589.1"
                     /translation="MKLAINLLLSLGMLALCVWLIWPDPKTRHELSQVFHAMNFREFA
                     PYLAGYIGLLVVVQLTRALRWNYLLAPLGVRIPPGPLLAISSVGFMAILALPARLGEF
                     VRPGLLRKRGISATSALGTVAVERIVDGLLVSLFVFGAFFALRGPSSPGWMMPTAYAA
                     LGIFALAMIFLVGALKWPEQSVQFWLRISLLPRISPRIAAIIERKALDMIKGFEVMRD
                     TRNLAWFVAYSVVYWTCNGLGVYILARGFGLDLGIVGAFATMGLVAVGITLPNSPGLV
                     GQFQWFTLLGLSLYIHGASTETSSIYPTTFAFANTHYGLQVAWYILCGAIGLATRYVS
                     FHDMWEARKTGDADPE"
     gene            13755..14222
                     /locus_tag="VMZ53_13850"
     CDS             13755..14222
                     /locus_tag="VMZ53_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29590.1"
                     /translation="MRRVLLALLFAVPVAGGTAHARSEKMLAYEREPAWAASVRFLRV
                     DAHLKLIEKDAEAGYVIFEYTEEKKTFRGSLELIETVKDGRKVVRFVMTIEDRPAWVE
                     IGLLTKLESKLRSELGSPAPAPSPPAKKDEPKKDEPKPDDKPKDDGPPVSDKP"
     gene            14249..15280
                     /locus_tag="VMZ53_13855"
     CDS             14249..15280
                     /locus_tag="VMZ53_13855"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HUS29591.1"
                     /translation="MSGFVDLRSDTVTRPSAAMRLAMANAEVGDDVFGDDPTIKKLEE
                     TVAGLLGMEAGLFVPSGTMANQIALRSQTRPGDEVIIGQSAHVWRHESGALAALAGVQ
                     TQVVGTDGTFTGADVRAAFKGSDPYQSPTTVVEVENTHGATGGTIWPRERLADVVATG
                     KQLGMALHLDGARIWNAAAATGASEKELAAGFDSVSVCLSKGLGAPVGSVVAASKPII
                     KACHRLRKMQGGGMRQAGILAAAGLFALEHNRARVVEDHANARAFAEFLAAVPGLRVD
                     LANVQTNIVMIDVERGTVAAVIELAKKAGVLIGANGQRQIRAVMHLDVDREGVMRAAR
                     VIAEIAAKL"
     gene            15280..16308
                     /locus_tag="VMZ53_13860"
     CDS             15280..16308
                     /locus_tag="VMZ53_13860"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS29592.1"
                     /translation="MRCALLALLVGCSAPATLPELVQAEKLADHGDTDGAVTAYREAQ
                     RKCKSLKPARRATQACGEALLGEANALADGGRTDAAIAAYVAIPNRAPDDPVTQSTAM
                     YRAGELLLKQGKTVEAWTVLWKTVTDWPDEALAGDALKTLVADGRTRNAQALADEIGK
                     LITPLAETGVADNLVWWLADLSEHELANVAAARSLYDRIPADWPKSGFRDDARWHAAR
                     LSLALKDPQGAVDRLRALLATREVALGAGSYFSIWLDDAQLKLGLVLRDELHDYAAAA
                     AALRKLPKDYPASILRDDALYELTVTLAKAADKPGACRAIADLRKQSPDSKYTDEASA
                     LSAELSCP"
     gene            16299..17123
                     /locus_tag="VMZ53_13865"
     CDS             16299..17123
                     /locus_tag="VMZ53_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS29593.1"
                     /translation="MSVTAIRMHDVHKRYGHRDVLDGVSLEVPRGGKLGLIGPAASGK
                     SVVLKLVCGLEQPDRGEIEVLGESVVGKREVQLGDLRQRIGMLFQNYALFDFLDVRGN
                     VAFPLEQRGGLKADEIATRVATRLRAVGLAGSEHKLTGELSGGMKKRVGIARATVADC
                     ELVIYDEPSAGLDPVTTQKIYDLLAEDQQRTGAAVLAVSSDVVALASFVDEIAFLYRG
                     RILYRGPAKTIADAPNAIVRQFVRGELEGPLDETRDGGHHDVPPETSLEMPAEPRE"
     gene            17120..17896
                     /locus_tag="VMZ53_13870"
     CDS             17120..17896
                     /locus_tag="VMZ53_13870"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS29594.1"
                     /translation="MIGERALSGIGGGAIRIAEEVGGILILTGQVLRALVPPRIDGRE
                     LMKNLYKMGVKSVPIVVLTAFFAGGLMTLQTGPFVKKLGATSLAGWGAGYAVLREIGP
                     ILIALMFSGRVGANNTAELATMTVTEQLDGLRALAIDPIRFLVVPRVIAMVVMLLALT
                     VIGDLVALTGASIVGRAMLDIDWSTMYYSFAENLQPYDFLHGVYKSIAFGAAIAVTSC
                     YFGVTVRGGAVGVGKAVNAAVVAAAVSIMLLDFFLTYTTG"
     gene            17896..>18682
                     /locus_tag="VMZ53_13875"
     CDS             17896..>18682
                     /locus_tag="VMZ53_13875"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS29595.1"
                     /translation="MTRVERIGESVLGVVRAGAELWMVYVSTIAGIIRGFSRRRGGRG
                     RPKGEIWRQLYGIGNKSLVFITVTIGFIGMVSTYQSCLQLSRVTGDYSQIGPQFARLI
                     VSDFGPTLTALMLATRVGAGIAAEIGSMKVTEQVDALRMSGVLPIDYLIVPRFIACIV
                     MTLMLAVLGSAIMYAAGGLTAQYSFGVNPNLFFDLSLLRFRHFALMIVKATAYGAAIP
                     VIAGFCGLRARGSSEGVGWATTAAVIGSSFAVIMLDFIISAAAL"
BASE COUNT         3120 a         6323 c         6018 g         3221 t
ORIGIN      
        1 gccgcagcgc cggcgtgatc gtcggcattc acgagcagcg gcagccaatg catcgcgatt
       61 tcgttgttcg tcgtcgacgt cagatgaaat gccgcgtcga gcgcgtcgag ctgcggagcc
      121 ttgatgtcct tcggcagcgc gcgcaggaac acgacccact ccatcgtggc ccacgcactc
      181 gcgtcgggga gcgtccccgt cgtcgcgaag gcattcgcgg ccgcagccag ccgcgtggcg
      241 atcgtggagc tcgagggtgc ggcgcccggc ggaatgccac ccttgtagag ccacgcatcg
      301 aggtcgacct ttgtaccgag cacgcgcttg gcctccgcga cgaacatgcg cgaatccacc
      361 gacgagaacg cgtggccgtc gaaccagccg cgcaagaacg cgtcgaacgt cgcacggccg
      421 tacgtcagct cgagcgtgcg catgagcagc gcgcctttgt cataggcgag gtcggccgga
      481 aaatcgtcgg gcgcgatgtc tcgtccgtag gcatgcgcga ggctcgtcgt ctgcaacgcg
      541 gagccagcct ctgcgacggt ctcgtcgata tcgcgcgacg cgatcgacca cagcagctcc
      601 gagtactcga cgccgcgaag ctcctccatg atccgatgct cgacgtacgt cgtgatgccc
      661 tcgttgagcc agacgtcgtt ccacgtcttg ttcgtaacga ggttgcccga ccacgaatgc
      721 gcgagctcgt gcgcgatgag gccgacgagc gcgcggtcgc ccgtgatcac cgtcggcgtc
      781 aagaacgtca ggttcggatt ctccatgccg ccatacggaa agctcggcgg caacacgagc
      841 atgtcgtagc gaccccagcg atacgggccg tacagcttct cggcggctgc catcatttgc
      901 tcgacctcgc cgaactcgtg cgcagccgca tcggcgattg ccggctctgc atagacgccg
      961 ctgcgtgggc ccgtcgcgcg aaatacgaag tctccgacgg cgagcgccat caggtacgac
     1021 ggaatcggct gcttctgctc gacgtgccac gcgtcgccgc tcggctcggc cgcattctct
     1081 cccgacatca gcgcgcgcat cccggccggc gcgtggatcg tcgctttgta cgaaaaccgg
     1141 acgctcggcg agtcttgcag cgggatccac gtgcgcgcgc gaatcgcttg cgactcggtg
     1201 aacagcatcg gcttcacgcc accagcggtc ccgcttggct cgacccacag cagggctcca
     1261 gcatccggcg ccgttcgata ggtgagctcg acgcactccg tcgctgcgcc cagatcgatg
     1321 cgcaggggcg cgccgatgtt gacgtgcttg ccgacgtgcc aaccgagctt cttgccggcg
     1381 cagtccgtca cgccctcgat cacgagtcct tcgtcgtcga gcacgagctg cgcgctgcga
     1441 tcgacacgcc ggagctcgag cttcgctgtt ccggcgagca ccttcttgtc gaagtcgacc
     1501 gtgagctcga gcgtcaacgc gcgcacgctc acgcgatcgg gctccgcgaa cgaatgcggg
     1561 tccaccggcg cgcgggctgc gctccgacac gccacgagcg tgcacgcgat cacgataggt
     1621 ctggcgttca tgcgcgggag gcccaaatcc tatatcgtcc gggcgatgcg tacactcgtt
     1681 ctcctaaccg tggcgctgct cgcaacgagc tgcgtgaaaa agtcgaccta ccagaaagcc
     1741 gtcgaagaga acaagcgcct caaaggcgag ctcgccgaca cgcagaagaa gctcgccgac
     1801 accgagcaga cgctgtccga caagatcaag gagctgcagg cccagctcgg catcctcgac
     1861 gagacgacga agaacaagga agccgagctc ggcaaggtgc gcaccgagaa agaagccacg
     1921 atggccgagc tcgccgagct gcgccgtcag aaggaagcgg cagagaagcg catcgccgcg
     1981 tacaaggcgc tgcaggacaa gttccgcgcg ctcaccgata ccggcaagct gcaggtcgtg
     2041 ttccgcaacg gtcagatgac gctcaagctg ccgtcgggga tcttgttccc atcgggctcc
     2101 gacaagctgt ccaaagacgg cgagaccgcg ctcgcggacg tcgccaagat cctcatggag
     2161 ttcaaagagc gccgcttcat cgtcgcgggc cacacggata accagccgat caagaccgcg
     2221 cagttcccga acaactggta cctgtcgacc gcgcgcgcaa actcggtcgt ccaattcatg
     2281 atcaagcagg gcttccccgc gaagagcctg gccgcctctg gttacggcga gttcgacccg
     2341 gtcgcggcga acgataccga caagaaccgc gagctcaacc gccgcatcga gatcattctc
     2401 gttccgaatc tcgaggagct gccgagcctg acgggcaatc agccctgacg ggataaagaa
     2461 gtatgaatcg acgagccacg ggcaaaaagc cgcgccatca tcaggctgtg agaacccttc
     2521 tcctcgccgc tctgcttttt gctgccgcct gcaaagacga caagaagccg tcgtcgagcg
     2581 aaggcgcgac tccgacggag cgctccggcg atcactccgc cgcggtctcg tccgcgaagc
     2641 acccggcgct gccgcacgat ggggacgacg tcggatccgg tgcacgccct cgcctccccg
     2701 gcgtcgacga ccagcgcccc gactggaacg atccggctat tcgcgccgag tgggaagaga
     2761 agcgcgaaga gcgccgcaag cgcatggaag ccgagctcga caccaacaag gacggcaagg
     2821 tcagccccga agagcacgct caacgcgtca agccgatgct cgagcgcatg gatcaaaaca
     2881 acgacgggaa gctgacgccc gacgagatgg ccgagtccga acgccggatg ggcttcgacg
     2941 atccggccgc gctcgatacc gacaagagcg gtgatatctc gctgatggag ctcgacacgg
     3001 cggtgaccgc tcgccgcgag aagatgcgta cgaagtggcg aggccgcacc ggcccgggca
     3061 tgggctccga cgagtagtcg ctacggcgcg gcgcagaggt cgatgtcgac ccacagcgga
     3121 tagtgatcgg agccgttcga ctgctcgacg cccgctgcgg tcacggcgac gccacgcgag
     3181 tacagattgt cgaggcgcat cgggaagccc ggaatcggga acgtgacggt attcggcgag
     3241 acgttgccgt cgaaccgacg cgacgccatg tagtcgtcga gcgcggcggc ttgcttcatg
     3301 ccgaggacgg cttccgagga cgtgagcggc acgatgccgt cgatccactg ccagggtgcg
     3361 gtgttgaaat cgccgccgat gactgccgcc tcgtcgagct cggagactgc ggggtcgagc
     3421 tggcgaatgc gatccgagat cgagatgcgc acgtcgaggt gcacgctgac gacccgcacg
     3481 cgcttgtcgc cgacgatgat atccgccgcg atcgcgccac gcggctgcgc gtgatacggc
     3541 tgctcgatgt acggcaggcg cttgaccatc acgcggtcga gcggatagcg gctcatgatc
     3601 gcgttcgcct gcatgccgcc gtcgccgaga tcgcgaatcg gttgatgcgc ccacgtcatc
     3661 gcgagcgctt cggcgagctg cgcggtgcgc ggcattggtt cggccgcgta tcccgatgac
     3721 tcttgcatca gcacgacgtc ggcgcggctg atctcttgcg tcgcgagcag cgcggtcgcg
     3781 agctcgttag cgtcgggcag cttgtgcacg ttccacgacg cgatgcgcag cgtgcagccg
     3841 ggtgtggcgc gcagtgccgg cgccggaccg atcgatacgc catttacact cgacgcgtct
     3901 tcccatgggc caccttgatc ggcgacatcg acgcaaccga tcaacgcgag cgcggcacaa
     3961 gcgagcttca tggacgtact ccgacggcga aaccgaacat cgggccgatg ccgccctcgc
     4021 ccgcgacgac cgagccgctc gcgcgaagca cgacgtgatt gcgcctgtac tcccagccga
     4081 gcgacgcgaa gccgccgccg cctgaatcgc tcatgccgtc ccagccttcg accggcgatg
     4141 gcagatggct atgcgcgagc accgcgccga cctcggtgaa gaatgcgtgg cgccgtcccg
     4201 cgacgatcgt cccgtgaacg tacggcgcga acgtgagcac ttgctgatcg ctgacgaccg
     4261 cgaacgagcc agcggcgccg agggcgagcc gcgacgtgat gccgtgctcg taaccgatac
     4321 cgtagagacc gcccttgccg agcgcctcga cgtagacgag gtgtttgctc ggctgcgcgt
     4381 cggcggtttc cgttatgccg tcctcagcga gtgccgcggt tggggtggaa agcaggagag
     4441 tcgcggcgat ccacctcatc aacgaggtag gagcctagca gctagcggcc gagaatctcg
     4501 tcggtcgtga tcgcgtagaa cgcgcggacg tccatcgtgg tgtcgggcgt gctggcattc
     4561 atcagcgggt cagcataatc cgcacggcgg tagctgtaag tgccttcgac gccgatggcc
     4621 tgatgcgtct tgatctgcac gcgcgcgcca agcgtggacg tcgcgacaaa ctcgttcgcg
     4681 tcatcgccga cgaggcgtcc gcgaatctcg tagaggcgcg tcgtcgaccg aacattccag
     4741 aggccgcgct gaccgccctg cagctcgagc agctgcgcca tacccggacc gtcgttgtga
     4801 tcgttcatcg ggccctcttc ctcattcacg ccacctgcgg cgccgtatgg aacgagatac
     4861 gccgcgcccg tgcccgcgac gtagccgcga tcaccgaggc gagcttcaac catgccgccc
     4921 ggaccgatgc cgagcatgct cgcgcgcacg aagtggttgc tgttgtagtc gtagccgctg
     4981 aagaggccgt acatgccgcg gacctgatcg ccacggacgc cactgccaac gagcatgccg
     5041 cgcacgtaga ggagcgcttc gagctcgtcg gtcgatgaat cgatcgcggt ctgcagcgtg
     5101 aagtgatcga gcgggcggcg cggctggaac gcacgatcgc ccatcacgcc gtgctcgaca
     5161 tacgcctcga cgtgcagctg gccgtcaccg ccgtgctcgg cgccgagcag atgcgtcggc
     5221 tgctgccagc caaccgcgaa gtgtgcgttg agcgcgggcg gaatcgtctt ggcccatgcg
     5281 tcgccccatg cggcgcggtt gatcgcaccg accggatcga tcaccgaggc cgcggtcttg
     5341 cggatccacg tcggcttgcc gtagccgcga tagagaagcg cgcggctgaa gcggtgcatc
     5401 gcttcgccga tgaacatgcc gccgatcggc gtcgtgatca tgtcgttcgt cgatgggatc
     5461 tcgttctcga cgagctccca caggaggctg cccgcgaacg agtacgcgcc ggcgacccag
     5521 aactcgtgac cggtcgaccg ggccgcagag aacgcgagcg agccggtgaa cgggtggccg
     5581 agttggttcg tcgagtacgc gtcgtcgtcg aatgcccagc cgccgctgag gtgttccttc
     5641 gtggtgtcga cgctgacgtg cgcccaatcc atgccggcga gatatgcacc cgtgaagatc
     5701 gcggcgttgc cgagcacgac ctcgagtgtc ggcacgaggt agctcttctg ctttaccggc
     5761 agatcctcgg gctcggtgac tgcgggttga tagacctgat cgctatcagg ttgagccgag
     5821 gccgtgcgcc caggccacac cgacgccacc aacgtgagac cgacgagtaa gcgtggaaac
     5881 atagaggcgt gcttttgcaa tgagcaggcc cgtcgaaacg cgcgaatggt caccactccg
     5941 acgcgtcaga tcgcgagcgc cgcgcgcaca cctagcaggg tcccaacacg cagccgactg
     6001 cgccgtcggt accgaacaca agaacagagt taggtgtgcc gggtccgctg ttcgcgatca
     6061 ccgtgcgccc gtgcgcagga cctgcgagct gcagaggatg gatcgtcgtc gagatcagcg
     6121 tgccgggcgt gtacgtgtag tcctcgacga gcgtgaactg tgtgtcgctg ccactggctt
     6181 gcacgatcaa gatatcgggc cctggagtca tcgtgatgtt cgcgccggcg agcagcgtga
     6241 cttcactggc gccgggcaac atcgggatcg cgagacacac cggcgtcacg ggcaccgtcg
     6301 acgcgccagg atcgacgaca cacacttgtt tgttcgcgag atcgagaccg aggatctctt
     6361 cgcgtccgtc tccgtcgaga tcgatcgatg tttggccaac ccatgcggga ccgttgcgga
     6421 cgaccgtcca cgcaaacatg ccgccgctcg gcccaggaac gctcgcgcgc tggatctgca
     6481 tgggcgtcgc aacgaccgcg atctgatcca taccggacgg atagctcgtc gcgagctgcg
     6541 cgaggtccca cgttggaccg ctcgccgcag ggatcggttt gacgtcgccg aggtttgccg
     6601 atgcgtgatt gatcgtgccg ccgttactga ccgcgatgtg gagcatggcg ccgggaaaat
     6661 tgatcgcgtg caaccacagc gtggcgttgc cagccggcac cgcggtcgtt tcccgcgcct
     6721 tctcgccgag gatgttcgag tactgcgtga tctcgatgtt gccgttgctc agatcgcccg
     6781 ggccgtcttc cgccgtcgcg acgtagatgc tcggtgcagc gccgccgagt tggagcgccg
     6841 cggttggaat cgcgagctcc gccggcacga acgtcgagaa cgatcgcacg ggcttgctct
     6901 gcccgcctcc aagatcctgg ccgccggtga gcagatacag accggctcgg ttctcgccgc
     6961 ccgttccata cacgacgatg tccgttgagc cgttgccatc gaggtcgccg gaggtcactg
     7021 cctgcacggt gacgagcgag ggcggccacg actgcgtcgc gccttccggc ggggagatga
     7081 tgcagcccga cgcgcacaca agcagcaaag cggtgcgcat cagaacggca cctgttcgcc
     7141 ggaatcacgc gacgccgcct tcttcttctt gaggaccagc ttcttcgtcg tatcactcgg
     7201 cccgacgacg agcttggctg tctgataacc ggacagactt gccgtgagct cgactttggc
     7261 gctgtcggcg ggaagctcga ggatgaacgg cgtcttgtac gtcgtgcctt tgtgcgtgat
     7321 cgacgcgcca ctcgggtgcg tatcgatcat gaccttggcg gtcttcgctt cgaccaccgc
     7381 tgcatcgacg acgaccgtcg actgtgcggc atcgggcgtc gccacggccg tctgcacgga
     7441 cgcatcgcgc ggtgcggtgc tcacctgcgg cgaccggttg ccggtcttca tgaacatgat
     7501 tgccgcgaca acaacgatcg ccagagccgc ggcgatcgcg agcaacgcga tccgacgtgc
     7561 gggacgcttg acctcgtcga ccatcgcagc gacggaatct gcgggcgcgg gcgtggcggt
     7621 cggcgagcgc gcggcgacag gcgcagcagg agctgagccc ggcggcggcg tctcgagctt
     7681 gcgcgtcgcg gcgtacggat cgctcttgcc gacgacagac ttctgttggt cgctcgagcc
     7741 acgcaggttc accgttgcgc tcgcgttcgg ctcctcgttg atcgtgtcgc cggcgttcgg
     7801 ctcgtcggac gtgctcagca tcgcgcgcgc ttgcgcagca gtcggacggc gatccggctc
     7861 tggctcgagc atggctttga tcgtcgacct gatcggcgca gggatgcgcg cgagcgccgc
     7921 ggcgttgtcc atcttctgcc cgatcagctg cggcaggttg tcgccttccc acagcggctt
     7981 gcccgtcgcg agctcgccga gaatcacgcc gagtgcataa acgtcaccgg ccggcagcga
     8041 cgttccgttc atcgccgcct ctggcgacat gtagcgcggc gtaccgacga cgatgcccga
     8101 ttcggtcgca cgcgagcccg gatcgcggat gttcttcgcg aggccgaagt cgagcacctt
     8161 cagcagatcg cgcccaggcg gatgatcgag cacgatcgcg ttctcgagct tgagatcgcg
     8221 atgcacgatg ccgagcgagt gcgcggcttc gagcgcgtcg cagatctgga tgccgatccg
     8281 cttcacccgc tcgatcggga acgcgccctc cgcctgcagc acctggtaca gcgtgcgacc
     8341 gcggataagc tccatcgcga tgaacaagcg accgtcatcc gcttggccga agtcgaagac
     8401 gctgacggta ttcggctgcg acagctggct cgcgagcctc gcttcgcgca agaaccgccg
     8461 cacgcccatc ggatcacgcg agaacgatcg atcgatgagc ttgatcgcga cctcgcgtcc
     8521 gaccgagcgc tgccacgcgc gatagacgct gcccataccg ccctgcccga tcttgtcgcg
     8581 gacctcgaag cgctgatcga tcacggtgcc gacgacctga tcggcttcat cgcgaatctc
     8641 gacgagcttc gagttgcacg tcggacacga cgtcacgtcg gcctgcacgt ccttgtcgca
     8701 cgtcgggcag atccgatgaa tcacacgccc gcttgtcgac ggcgccgagc tcaccgcgtt
     8761 tctccgaacg tgccactgaa gctgatgccg atatcgcccg gcccgccgcc gacctggatc
     8821 ttgccgggct tgctcacggg cgtatcaccc gccaccaaga agtagatata gaaaccggcg
     8881 ctgacgacgc tcacacctgc gcagatcgtc gtcgcgatcg cgaagttgtc gaacacctgc
     8941 ttctgctctt cgaacgcagg gtccgtcggg aacaagccat tcgcgtcgag cttcgtcttg
     9001 tatgcgagcc cgccgagcag cgcggccgac aagcccgacg acagcatcgt tacgagcgcg
     9061 atcttcttcg cgccaaccgg acgaccgttg acgcgcacga acgaattgtc gacgcccgca
     9121 tctggcgcaa cgaccggctc gatcggtgta gcgacaaccg ccgcatcggg cgtcacgctc
     9181 ggcgttacga tcggcacgcc tgcatcgggc gccggcggcg gctcgacctt cggctcttcg
     9241 aggacgatgg cgacgcggct caccggtgcg gcaatgctcg cctgcgcggc cttcggcagc
     9301 cgcccttgcg cgctcgccga gaacatcacg acgcccggct tcagccacac cgtccgcatc
     9361 ccgcgcatcg caatcccgtt gacctcgacg atcgcatcgg acggcgcgac atcgatcgtc
     9421 gtcggcacgt agccgccctt gcgaagcgca tcggacagct gcgtcgagac ctctcccgta
     9481 caccacttcg gtcgcacgcc attgcgcagc gccgccaaat cccagacgag ctgcgccttc
     9541 gtcagctcac cactgcgcaa gtacgcgagc gcgaggttgc agtcatgcac cgccgctggc
     9601 cacttgcgcg cgactgcctc gagcacagca gcggcctcgc cgaactgctt cttcttcgcc
     9661 agcgcctgtg cacgctcgac gtccgcagcg ccgagcttct gcgcgtcagc cgcctcgggc
     9721 cccggctcga tgatccatcg ctcgtccgcc gagccgatac tcgtgccgag cgcgatggcg
     9781 atcgccgcgg cggcgagagt cacgacgaaa aagcgcagca tccccctgga gagccaaggg
     9841 tatcactgcc agcccgcagg cgcgcgcggc tacttcttct tacccgtgtc cttgcacacg
     9901 acctggccga cgcaattcga gacgtcgacg ggtctgttgc gctcgtcgct gagccgcttc
     9961 tgctcgagga gccacgcctc gtgcttcgcc gcgtcttccg ccttcttctc gtccgcgagc
    10021 ttcttcttga tctcctccgc cttcgccgcg tcgatcttgc cttgcagctc gagctcgagc
    10081 gctgcaacgc gcttcgcgcg ctcgtcggac tcgagcttca gcttgtcgag tccgcgctgc
    10141 gcttcggctg cgtcctgcct cgcttgctcc tgcctcagct gtgcggcgcg cgccgcttgc
    10201 tccgccttcg cagtgtcctg cgcgcggttg tacgcgaaca cgccgaggcc gatcgacgcg
    10261 acaaacgcga ccgcggtcac cgcgaccatc cacgtcggtc gcttgcgcgc gacctcttcg
    10321 cgacgcagct cgacctcggc cgccatgcgc tgcgcctcga gctcggccgc cagacgcgcg
    10381 cgctccgctg cttccgccgc ttcgacgcgc agccgcgcct gcttctcgcg ctcggcctgc
    10441 gcgagctgga tcttgatgat ctcttcgcgc tcggcgcgct tgcgcgcttc ttccgcgtcg
    10501 accttcgcct gttcggccgc tcgctttgcc tcgatctctg cattgcgcgc acgctcggct
    10561 gcgttgcgct cgtccgcgat gcgctgcttc tcgatgtcgc gcagctcgga caacgacgtc
    10621 atgatgctgg tctcgatcct gtcccgcatg cctagaagac acgcggcccg aatcgagatt
    10681 gcgcggctgc tacccgaccg ctgcggcgcg gctagcgggt gatgtcgtcg ggaccagaaa
    10741 tcctgacgcc atcggcacat ccgcgacgat gcccgaactt cggcggcggg acgtggacct
    10801 tcgagtgatg cgcgtggagc cgatgctcct ttgccggcgc cggccggctg gcctcgacat
    10861 acgccctcat caccgccacc tgtccgacga gcacgagcac cgcgatgaag attccagcga
    10921 tcggcgtgcg aacggccagc atgtctaaag cagaccagct tccgtcgacg cgcgcaacgc
    10981 gaaaacgggg tccgccgaaa atcaaaacgg cgccccgatc gagatcgacg cgccgcgtga
    11041 gccgaacccg gcgaggggtt agctgcagcc cttggcgagc gggttgtcga ggcactcctt
    11101 cgagaccttg actcccttgc ggcgctcggc cttttcggcg gcgtcgcgag cggccgcggc
    11161 gcgctgcttc atctcgaact gctctctctg gagagcgacg aggcgagcct tcgcggacgc
    11221 gcgatcggcg tcgttttggg catcggcgac cgcgctcacg gctgcgccga ccttcgtatc
    11281 cagatcgtcg aggtccttca tgatgcggtc gagcttgtcc ttcgcatcat tggcgtcctt
    11341 cacggcttgc gccgcctcgg cctccgcctt cttacggcga acatcggatg cctcggactc
    11401 tctcatgcgg ttgaccgcga agaagatgag gccgatcgcg ccgacgagcg cgatgcccgt
    11461 gaccgcgagc atccacgtcg ggcgcttctt ggcgacctcg gcgcggcgca gctccatttc
    11521 ctgctgcagg cgcgattgct cgagcgccgc ttggtgacgc tgacgctcgg cggcttcagc
    11581 agcctcgacg cgcatgcgtg cttcgcgctc ggcgttcact cgcgcttctt cgatctggcg
    11641 ctggtgatcg cgctccgcct ggatcttcgc ttcctcttcc tcgcgcttgc ggcgctcggc
    11701 ttcttctcgg gcgcgaatgc gtgcctcttc ggcggtcttt actgcactcg cttcctcttg
    11761 gacgcggttc tcctcgatct ggcgaagctc gcgcagggag aacagaactg aattttcgcg
    11821 cttctcaccc tgtgccatgg atgaacctct gctcggtgac ccgactctcg atctctaaca
    11881 tgcacgatct ctccgggcaa tgaggtcgcg cacttggact tcgaaccagc attgtacgct
    11941 catcccgagt tgggctacac cccacatcgc gcatgggctt cgtcgatctc catagccacg
    12001 tcctctatgg cctagacgac ggcgctcccg acgaaacgac cgcgctggcg atgctcgacg
    12061 ggctaactac gctcggcatc agcgagcagt gtctgacgcc ccatcagaag gcgtcgcagt
    12121 accttccagc gtgggatcga atcgaggcca cgatggccaa gctggagcag gtgcgcaaac
    12181 cgcaccaccc gacgctccgg ctcgccgccg agaacatgtg ggacgatgtg ttctacaagc
    12241 gcgtcgtcga ggatgcgatc cccggttatc gcaataccgt tgcgttcctc gtcgagctcc
    12301 cgccgcccgt catgccgccc gggatcatcg agcagatgtt caagtggcgg atggctggca
    12361 aggtcccggt gctcgcgcat cccgagcgct atcacgcact ctgggacaat gacgacctcg
    12421 cccagaagct gcgcacccac tgcgcgttct tgatcgacct cggcgccctc ggtggcttcc
    12481 acggcaagcg cgagatgaag gccgcgcgcc acctctgcga gaagggcctc gtcaccgcgg
    12541 tcgccaccga tgcgcatcag ctcggcgatc tgcagcaagc cgtcgaaggt ctgcgctggg
    12601 tcgagaagaa gctgggagcc gccgcagtca cgcgcctctt cgatcacgcg ccgcgcgcga
    12661 tcctcgccgg cgagcttccg gactgagatg aagctcgcga tcaatctgct gctgtcgctc
    12721 ggcatgctcg cgctgtgcgt ctggctgatc tggccggatc ccaagacgcg acacgagctc
    12781 tcgcaggtct tccacgcgat gaatttccgc gagttcgcgc cgtacctcgc gggctacatc
    12841 gggttgctcg tcgtcgtgca gctcacgcgc gcgctgcgct ggaactacct cctcgcgcca
    12901 ctcggcgtgc gcatcccgcc cgggccattg ctcgcgatct cgagcgtcgg cttcatggcg
    12961 atcctcgcgc tgcccgcgcg cctcggcgaa ttcgtccgcc ccggtctgct gcgcaagcgc
    13021 ggcatctcgg cgacgtcagc gctcggcacg gtcgcggtcg agaggatcgt cgacggcctg
    13081 ctcgtctcgc tgttcgtgtt cggcgcgttc ttcgcgctgc gcggtccgag ctcgccgggc
    13141 tggatgatgc ccactgcgta cgccgcgctc ggcatctttg cgctcgcgat gatcttcctc
    13201 gtcggtgcgc tcaagtggcc ggagcagagc gtgcagttct ggctgcgcat ctcgctcttg
    13261 ccccgcatct cgccacgcat tgccgcgatc atcgaacgca aagcgctcga catgatcaaa
    13321 ggcttcgaag tcatgcgcga cacgcgaaac ctcgcgtggt tcgtcgcgta cagcgtcgtc
    13381 tactggacct gcaacggcct tggcgtctac atcctcgcgc gcggctttgg cctcgacctc
    13441 ggtatcgtcg gtgcattcgc gacgatgggc ctcgtcgcgg tcggcatcac gctgccgaac
    13501 tcgcccggcc tcgtcggcca attccagtgg ttcacgctgc tcggcctctc gctctacatc
    13561 cacggcgcgt cgacggagac gagctcgatc tatccgacga ccttcgcatt cgcgaacacg
    13621 cactacggcc ttcaggtcgc ctggtacatc ctgtgcggcg ccattgggct ggcaacccgc
    13681 tacgtgtcgt tccacgacat gtgggaagcc cgaaaaactg gcgacgcgga cccggagtag
    13741 cgaccatcgg gcagatgcgc cgcgtgttgc tcgccctgct cttcgccgtc cccgttgccg
    13801 gcggcaccgc gcacgcgcgc tccgagaaga tgctcgcgta cgagcgagag cccgcatggg
    13861 ccgcatccgt ccgttttctg cgcgtcgatg cgcacctgaa gctgatcgag aaggacgccg
    13921 aagcgggcta cgtcatcttc gaatacaccg aggagaagaa gacgtttcgc ggctcgctcg
    13981 agctcatcga gacggtcaag gacggccgca aggtcgtgcg cttcgtgatg acgatcgagg
    14041 atcgtccggc gtgggtcgaa attggcctgc tcacgaagct cgagtccaag ctccgctccg
    14101 agctcggctc tccggccccc gccccgtcgc cgccggcgaa gaaggacgag ccgaaaaaag
    14161 acgagccgaa gcccgacgac aagccgaagg atgatggtcc gccggtgagc gacaagccgt
    14221 gaattcgcgt ctgcggtagg ctcgccgcat gtcagggttc gtcgacctgc gcagcgatac
    14281 cgtcacgcgt ccgagcgccg cgatgcggct ggcgatggca aacgccgaag tcggcgatga
    14341 tgtgttcggc gacgacccca cgatcaagaa gctcgaagag acggtcgccg ggctgctcgg
    14401 catggaagcc ggactcttcg tcccgagcgg cacgatggca aatcagatcg cgctgcgcag
    14461 tcaaactcgc cccggcgatg aagtgatcat cgggcaaagc gcacatgtct ggcgtcacga
    14521 gtcgggcgcg ctcgcagcgc tcgccggcgt ccaaacgcaa gtcgtcggca ccgacggcac
    14581 gttcaccggc gcagacgtgc gcgctgcatt caagggaagc gatccgtacc aatcgccgac
    14641 caccgtcgtc gaagtcgaga acacgcacgg agcgacgggc ggcacgatct ggccacgcga
    14701 gcggctcgcc gatgtcgtcg caacgggcaa gcagctcggc atggcgctgc atctcgacgg
    14761 cgcacgcatc tggaacgcgg cggccgcgac cggcgcgagt gagaaggagc tcgccgcggg
    14821 cttcgattcg gtgagcgtgt gcctgtcgaa gggcctcggt gcgccggtcg gttccgtcgt
    14881 cgctgcgagc aagccgatca tcaaggcctg tcatcggctg cgcaagatgc agggtggcgg
    14941 catgcggcaa gcaggcatcc tcgccgcagc gggcctgttc gcgctcgagc acaatcgcgc
    15001 gcgtgtcgtc gaggatcacg cgaacgcccg cgcgtttgcc gagtttctcg cggcggtgcc
    15061 gggcctgcgt gtcgacctag cgaacgtcca gacgaacatc gtgatgatcg atgtcgagcg
    15121 cggcaccgtc gcagccgtca tcgagctcgc gaagaaagca ggcgtgctca tcggtgcgaa
    15181 tggccagcgc cagattcgcg cggtgatgca cctcgatgtc gatcgcgaag gcgtgatgcg
    15241 cgccgcgcgc gtgatcgcgg agatcgccgc gaagctgtga tgcggtgtgc gctgctcgcg
    15301 ctgctggttg gttgctcggc gccggcaacg ctgccggagc tagtgcaagc ggagaagctc
    15361 gcggatcacg gtgacaccga cggcgcggtc accgcgtatc gagaagcaca gcgcaagtgc
    15421 aagagcttga agcccgcgcg ccgcgcgacc caggcatgtg gcgaagcgct gctcggcgaa
    15481 gcgaacgcac tcgcagacgg tggccgcacc gacgcggcga tcgcggcgta cgtcgcgatt
    15541 cccaatcgcg cgcccgacga tccagtgacg caatcgaccg cgatgtatcg cgccggcgag
    15601 ctactgctga agcaaggcaa gacggtcgag gcgtggaccg tgctgtggaa gacagtgacg
    15661 gactggcccg atgaggcgct cgcgggagac gcgctgaaga cgctcgtcgc agacggccgc
    15721 acgcggaacg cgcaggcgct cgccgacgag atcggtaagc tcatcacgcc gctcgccgag
    15781 accggcgtcg cagacaacct cgtgtggtgg ctcgccgacc tcagcgagca cgagctggcc
    15841 aatgtcgcag cggcgcgctc gctctacgat cgcattcccg ccgattggcc caagagcggc
    15901 tttcgcgacg atgctcgctg gcacgcggcc cgcctctcgc tcgcgctgaa ggatccgcaa
    15961 ggtgcggtcg accggctgcg cgcattgctc gcgacgcgag aggtcgcgct cggcgcggga
    16021 agctacttct cgatttggct cgacgatgca cagctcaagc tcggcctcgt gctgcgcgac
    16081 gagctgcacg attacgccgc tgctgctgcc gcgttgcgca agctgccgaa ggactatccc
    16141 gcttcgatcc tgcgcgacga cgcgctgtac gagctcacgg tcacgctcgc caaggccgcg
    16201 gacaagccgg gcgcgtgtcg agcgatcgcg gatctgcgca agcagtcgcc cgactcgaag
    16261 tacaccgacg aggcctcggc cctctccgcg gagctgtcat gtccgtaacc gcgattcgca
    16321 tgcacgacgt gcacaagcgg tacggccatc gcgatgtgct cgacggcgtg tcgctcgaag
    16381 taccgcgcgg aggcaagctc ggcctcatcg ggcccgcggc gtcgggcaag agcgtcgtgc
    16441 tcaagctcgt gtgcggtctc gagcagcccg atcgcggaga gatcgaagtg ctcggcgaat
    16501 ccgtcgtcgg caagcgcgag gtccagctcg gcgatctgcg gcagcgtatc ggcatgctgt
    16561 tccagaacta tgcgctgttc gattttctcg acgtccgagg caatgtcgcg ttcccgctcg
    16621 agcagcgcgg cggcctgaaa gccgacgaga tcgccacccg cgtcgccacg cggctgcgcg
    16681 ccgtcggcct cgcaggaagc gagcacaagc tgaccggcga gctgtcgggc ggcatgaaga
    16741 agcgcgtggg catcgcgcgc gcgaccgtcg ccgattgcga gctcgtgatc tacgacgagc
    16801 cgtccgccgg cctcgatcca gtcacgacgc agaagatcta cgatctgctc gccgaagatc
    16861 agcagcgaac cggcgcggcg gtgctcgcgg tatcgtccga cgtggtggcg ctcgcgagct
    16921 tcgtcgacga gatcgcattt ctgtatcgcg gtcgtatcct ctatcgcggt cccgcgaaga
    16981 cgatcgccga cgcgcccaac gcgatcgttc gtcagttcgt gcgcggcgag ctcgaaggcc
    17041 cgctcgacga aacgcgcgat ggcggccacc acgatgtgcc acccgagaca tcgctcgaga
    17101 tgcccgcgga gccacgcgaa tgatcggcga gcgcgcactc tcgggcatcg gcggcggcgc
    17161 gattcgcatc gctgaagaag tcggcggcat cctgatcctc acgggccagg tgctgcgcgc
    17221 gctcgttccg ccccgcatcg acgggcgcga gctcatgaag aacctctaca agatgggcgt
    17281 gaagagcgtg cccatcgtcg tgctcacggc gttcttcgcc ggcggcctca tgacgctgca
    17341 gacgggcccg ttcgtgaaga agctcggcgc gacgtcgctc gcgggttggg gcgcgggcta
    17401 cgcggtgttg cgcgaaatcg gcccgatcct gatcgcgctg atgttctcgg ggcgcgtcgg
    17461 cgcgaacaac acagccgagc tcgcgacgat gacggtcacc gagcagctcg acggattgcg
    17521 cgcgctggcg atcgatccca ttcgcttcct cgtcgtgccg cgcgtcatcg cgatggtcgt
    17581 catgttgctc gcgctcacgg tgatcggcga cctcgttgcg ctcacgggcg catcgatcgt
    17641 cggccgcgcg atgctcgata tcgattggtc gaccatgtac tactcgttcg ccgagaatct
    17701 gcagccgtac gacttcttgc atggcgtcta caagtccatc gcgttcggcg ccgcgatcgc
    17761 ggtcacgagc tgctacttcg gcgtgaccgt gcgcggtggc gcggtcggtg ttggcaaggc
    17821 ggtcaatgcg gcggtggttg ccgcagccgt ctcgatcatg ctcctcgatt tcttcctgac
    17881 atacacgact ggctgatgac ccgcgtcgaa cgcataggcg agagcgtgct cggtgtcgtg
    17941 cgagcgggcg ccgagctatg gatggtctac gtgtcgacga tcgcgggcat catccgcggc
    18001 ttctcgcgtc gacgcggcgg gcgcgggcgc cccaagggcg agatctggcg ccagctctac
    18061 ggaatcggca acaagtcgct cgtcttcatc acggtgacga tcggcttcat cggaatggtg
    18121 agcacgtacc agtcgtgcct gcagctctcg cgcgtcacgg gcgactactc gcagatcgga
    18181 ccgcagttcg cgcgcctcat cgtcagcgac ttcggcccaa cactcaccgc gctcatgctc
    18241 gcgacgcgcg tcggcgcggg catcgccgcc gaaatcggct cgatgaaagt caccgagcaa
    18301 gtcgatgcgt tgcgcatgag cggcgtgttg ccgatcgatt acctcatcgt gccgcgcttc
    18361 atcgcatgca tcgtgatgac gctgatgctc gcggtcctcg gctcggcgat catgtacgcc
    18421 gcgggcgggc tcaccgcgca atactcgttc ggcgtcaatc cgaacctctt cttcgatctt
    18481 tcgctgcttc ggttccgcca cttcgcactg atgatcgtga aagcgacggc ctatggcgcc
    18541 gcgatccctg tcatcgccgg attctgcggc ctgcgcgcgc gtggcagctc ggaaggcgtt
    18601 ggttgggcta cgaccgcggc tgtcatcggc tcgagcttcg cggtcatcat gctcgacttc
    18661 atcatctccg cggccgcgct ct
//
LOCUS       DATLBZ010000263         8124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_286759, whole genome shotgun sequence.
ACCESSION   DATLBZ010000263 DATLBZ010000000
VERSION     DATLBZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286759"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..1555
                     /locus_tag="VMZ53_13880"
     CDS             323..1555
                     /locus_tag="VMZ53_13880"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HUS29596.1"
                     /translation="MHTQADLLALADQVLLKNNKPQPIVFERGRGCEVWDRAGNRYLD
                     MTAGIAVCGLGHGHSLYTARVTEQLLKLVHISNLYYNDQQILAAKAIVDRSFADRVFF
                     CNSGAEANEGALKLARRYQAVVAGAPQRTKIVSTIGSFHGRTFATVAITGQPKYRENF
                     GPLVEPVVHIPYGDLEAAAKALEARDACCIIVEPIQAEGGIIVPPAGYLAGLRKLCDD
                     TGTILIFDEVQTGIGRTGKWFGHEHDNVKPDVMTLAKGLGGGVPIGAVAMSEKAAAGL
                     AQQAGGAVPHATTFGGNPLACASAIAVLEIIDSEGLLDRVAQAGEYLGSRLAELVKEF
                     PGHVTEARGRGLLRGVAVTGAPAQITTRAREKGMLLSIAGANVIRFAPPYIVERHQLD
                     EAVKILRDVLAEGHGKAA"
     gene            1555..2475
                     /gene="argF"
                     /locus_tag="VMZ53_13885"
     CDS             1555..2475
                     /gene="argF"
                     /locus_tag="VMZ53_13885"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HUS29597.1"
                     /translation="MPRGFLTLGDVPVAEWPALWDRAAYLKANRVQKTLAGKSIALVF
                     EKASTRTRVSFEVGVFELGGHAVVLSSQGSQIARGEPIEDTARVLSGYCQGIVLRTFG
                     QERVATLAKHATVPVVNGLSDQHHPCQVATDLFTVREHFGRIDGLRYAWIGDGNNMAA
                     SWIEAAGMFGLDLAIACPEGFAPDPLLVKAARDLQARNGRGSLTLTTDPKQAAAGAHV
                     LSTDVWASMGQEDDAARRKLAFTGYCIDAALLKRGAPDAMVLHCLPAHRGEEITGDVL
                     DGPKSLAWVEAENRLHVGKAILEHVMSVAG"
     gene            2488..3876
                     /locus_tag="VMZ53_13890"
     CDS             2488..3876
                     /locus_tag="VMZ53_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HUS29598.1"
                     /translation="MSSAVPPEIAGLGSSAMKLRVSPGFDPLKAQVGPDEYFVLSRID
                     GAMTLRDVLLSTGLPVERGIAIVMKLRSIGALLLPGETSAPVPAAAPTKPSQPRIATQ
                     TPSAPVTPARGVAVVTDLGRAPTVNVLPENIPAISRTATPATPMPASKMPARPLPPPA
                     KAIDLDPPTARGNDAGTVRGMPNVGRTPASTSKAPPSPEGLDAPTVTRAPRADINLVL
                     PQPTDVERAALAEPGDLGPDDRLKILAMARLLDAKDPWALLGVPRGADAKMLKRAYFK
                     LSKDIHPDRYYGRSLGSFAERLTVVFEAVSRAYARLTSPEKSKGSGAHVAVNAEQPQS
                     PQEYASELFERACGLEVQGDALGAMKLFAAVVRIDGQTRYLRRAASCALAAGQPKTAL
                     EYAKKAQTQAPQDPSSARLLAQAFRAAGKLGDAEEVLVMAMALKSENDVLTAELRSDL
                     AEVRRQIAAENR"
     gene            3959..5800
                     /gene="dnaK"
                     /locus_tag="VMZ53_13895"
     CDS             3959..5800
                     /gene="dnaK"
                     /locus_tag="VMZ53_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HUS29599.1"
                     /translation="MPSDKVIGIDLGTTNSCVAIVEDGTPSVIPNRGGYKTTPSMVAI
                     AENGKRLVGHIAKRQAITNAENTVYAAKRLIGRKWGSAAVRSSLEIMPYRIVEGPHDD
                     VRIMLRDQVFSVPEVSAYILQEMKLISEEYLGEEVQKAVITVPAYFNDGQRQATKDAG
                     RIAGLDVIRIINEPTAAALAYGFGRNIERKVAVYDLGGGTFDISILEIGNGVFEVIST
                     AGDTFLGGEDFDRRIIDWLVFGFEKQHGIDLRKDKMALQRLKDVAEKAKCELSSVKET
                     EINLPFIISTGRNEALHLQTMLSREKLEELTSDLVDRTVEICARTLEEAEIEKKQIED
                     VILVGGMTRMPRVQQAVAEFFGLEPSKGVHPDEVVALGAAIQGAALVADDERSGDILL
                     LDVTPHSLGIMIVGGYFHKLIEQNTTVPTSESHVFTTVKDNQTSVKILVLQGESDRAE
                     ENELLGEFVLTGLRRAPRGEVEIEVQFHISADGIVSVKAKDLETSLEQSITVTATSGL
                     TEDEIKKMIDANKDYMVEVRTGQEFDKARHQAEKSLEEIEALFPQVTDAIAGSDFGQD
                     AVKKARAVVERTRQAIQAKDLTTLADATEALNRTLNMFRGVVSKTRG"
     gene            5807..7327
                     /locus_tag="VMZ53_13900"
     CDS             5807..7327
                     /locus_tag="VMZ53_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29600.1"
                     /translation="MPKQSDIDRLIELGLNRYGAGEIDEALLMWEQALAADPDNPQAN
                     SYVDYVRSNYELLTSGEPALVDDPSYALDEEPDYQIEILPGELPRPSQPLLSLADEEL
                     DAGWDLEETQDAGGPMQRNYGDSFDEDEGGTREYYNAANPPSDFAAESETSEFQQREV
                     TGSFQNEGTPVGFAQQETSVRQRELGFVKPQGAPQPVQDKPSSPMAIGTASTVELGAA
                     LEAAGDNNPFAEVTLDRSLENQQTKETVLPKRSTRDQLEQSLRPPARRTSEELSQAEV
                     MLPHAPTQDFDGHGRIDVGAPTRELGLRPPARLSNPVDEDAPTGEADVRAIREAAGRR
                     EGNARPVSEGTRHDIVLQFDPIAARSAQVLAEVDADAPPDEPKEEQTRRRITMLLQKA
                     IEYNAAHETDKAVTAVDLALAEDPNSALGQKLVTRNKDTIMQIFQSFIGDLDRQPHLA
                     RPLHELAKEPISPRAAFLLSRIDGTLTVDELLDVSGMPRLEAYRHLCQLFLRGILR"
     gene            7330..>8124
                     /locus_tag="VMZ53_13905"
     CDS             7330..>8124
                     /locus_tag="VMZ53_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HUS29601.1"
                     /translation="MLVTRVTNMTTSRPHAGSALLRVVDGPDRGLELELPHAGVVLGT
                     DKACDVVLKDPFVSRRHCSVAPHAQGFAISDLGSSNGTVIDGVAVGKVVAPPGVALRI
                     GKTLVQLMPADEVIDIPPSTSDRFGALLGASHVMRQVFALLERASKSNAPVLFLGESG
                     TGKELMARGVHDASPRHDGPFVVFDCGASTDALIESDLFGHLKGAFTGAASDRQGAFA
                     AAHGGTLFLDEIGDLPVALQPKLLRMLEAGEVVPLGGRKSERYDVRI"
BASE COUNT         1496 a         2694 c         2700 g         1234 t
ORIGIN      
        1 cgtgatccat cggcgcctgc ggatgcaccg acggaacgct gaacgagaac gcggctgcca
       61 cgtagacgcc atcgtcggcg tgcgcggtcg cggagccagc tagcagaaag gggagggcaa
      121 gggcgagagg agcgagcttc atggcgcagc accgtcgcaa cggacgtgcc gcgcgtgcgc
      181 tccgctactt gccgctgact cgtgaaccag ccgtcacacg taactcgata caccgccttg
      241 cgatctgaca ggtcctgacg gccggagcgc tcgcgtcatc gagattgtta cctcctaact
      301 tcggcagtga tacaccttcg tcgtgcacac gcaggccgac cttcttgctc tcgccgacca
      361 ggtcctcctc aagaacaaca agccgcagcc gatcgtcttc gagcgcgggc gcggatgcga
      421 agtctgggat cgcgcgggca accgctacct cgacatgacg gcaggcatcg ccgtgtgcgg
      481 gctggggcac gggcactcgc tgtacaccgc gcgcgtgacc gagcagctgc tcaagctcgt
      541 tcacatctcg aacctctact acaacgatca gcagatcctc gcggcgaagg cgatcgtcga
      601 tcgctccttt gcggatcgcg tgttcttctg caactccggc gcagaagcga acgaaggcgc
      661 gttgaaactc gcgcgtcgct atcaagcggt ggtcgctggc gcgccgcagc gcacgaagat
      721 cgtctcgacg attggcagct tccatggccg cacgttcgcg accgtcgcga tcaccgggca
      781 gccgaagtat cgcgagaact ttggcccgct cgtcgagccc gtcgttcaca tcccgtacgg
      841 cgatctcgaa gcggccgcca aggcgctcga agcacgcgac gcgtgctgca tcatcgtcga
      901 accgatccaa gccgagggcg gcatcatcgt tcctccggcc ggctacctcg cgggcttgcg
      961 caagctctgc gacgacaccg ggacgatcct catcttcgac gaagtgcaga cgggcatcgg
     1021 ccgcaccggc aagtggttcg ggcacgagca cgacaacgtc aagcctgacg tcatgacgct
     1081 cgcgaagggc cttggcggtg gcgtgccgat cggcgccgtc gcgatgagcg aaaaggcggc
     1141 cgccggtttg gcgcaacagg ctggtggcgc agtgccgcac gcgacgacgt tcggcggaaa
     1201 tccgctcgca tgcgcgtcgg cgattgcggt gctcgagatc atcgacagcg agggcctgct
     1261 cgatcgcgtc gcgcaagccg gcgagtacct cggcagccgg ctcgccgagc tggtgaaaga
     1321 gttccctggt cacgtcaccg aagcgcgcgg ccgcgggttg ctccgcggtg tcgcggtgac
     1381 cggcgcgcct gcgcagatca cgacgcgtgc gcgcgagaag ggcatgctgc tctcgatcgc
     1441 gggtgcgaac gtgatccgct tcgcgccgcc gtacatcgtc gagcgccatc agctcgacga
     1501 ggctgtgaag atcctccgcg atgtgctcgc ggaaggccac gggaaggcag cctgatgccg
     1561 cgcgggtttc tcacgctcgg cgacgttccc gtcgcagaat ggcctgcgct ttgggatcgc
     1621 gccgcgtatc tcaaggcgaa ccgcgtgcag aagacgctcg ccggaaaatc gatcgcgctc
     1681 gtgttcgaaa aagcgtcgac gcgcacgcgc gtatcgttcg aggtcggcgt gttcgagctc
     1741 ggcggccacg cagtcgtgtt gtcgtcgcag ggcagccaga tcgcgcgcgg cgaaccgatc
     1801 gaagacacgg cgcgcgtcct ctcgggctat tgccagggca tcgtgctgcg cacgtttggc
     1861 caagagcgcg tcgccacgct cgcgaagcac gcgacggtgc ccgtcgtgaa cgggctctcc
     1921 gatcagcacc atccgtgtca ggtcgcgacg gatctcttca ccgtccgcga gcacttcggc
     1981 cgcatcgatg gccttcgcta cgcgtggatc ggcgacggca acaacatggc ggcgtcgtgg
     2041 atcgaagcgg ccggcatgtt cggcctcgac ctcgcgatcg catgtcccga gggcttcgcg
     2101 cccgatccgt tgctagtcaa agcagctcgc gatctacagg cgcgcaacgg ccgcgggtcg
     2161 ctcacgctga cgacggatcc gaagcaggct gccgccggcg cgcacgtgct ctcgacggac
     2221 gtgtgggcgt cgatggggca ggaggacgac gcggcccggc gcaagctcgc gttcacgggc
     2281 tactgcatcg atgcggcgct gctgaaacgc ggcgcgccgg atgcgatggt gcttcactgc
     2341 ctgccggccc accgcggtga ggagatcact ggcgacgtcc tcgacggtcc gaagtcgcta
     2401 gcttgggtgg aagccgagaa ccggctccac gtcggcaagg cgattctcga gcatgtgatg
     2461 tccgtcgccg ggtgatagag tgcggctgtg agtagtgcgg taccgcccga gattgcggga
     2521 ctgggttcgt cggccatgaa gctccgcgtg agcccgggct ttgatccgct caaagcgcag
     2581 gtcggccccg acgaatactt cgtgctctcc cgcatcgacg gcgcgatgac attgcgcgac
     2641 gtgctgctgt cgaccggcct tcccgtcgag cgtggcatcg cgatcgtcat gaagctccgc
     2701 tcgatcggcg cgctgctcct cccgggtgag acgagcgcgc ctgtgcccgc cgccgcaccg
     2761 acgaagcctt cgcagccgcg aatcgcgacg cagacgccat ccgcaccggt cacgccagcg
     2821 cgcggcgtcg cagtcgtcac ggatctcgga cgcgcgccga cggtaaacgt cctgcccgag
     2881 aacatccccg cgatctcgcg cacggcgacg cctgccacgc cgatgcccgc gagcaagatg
     2941 cctgcgcgcc cgctgccgcc gccggccaaa gccatcgatc tcgatccacc gacggcgcgt
     3001 ggcaacgacg ctggcaccgt acgcggcatg ccgaacgtcg gacgcacgcc ggcgagcacg
     3061 tcaaaagcgc cgccgtctcc cgaagggctc gacgcgccga cggtcactcg cgcaccgcgc
     3121 gccgatatca acctcgtctt gccgcagccg accgacgtcg aacgcgccgc actcgcggag
     3181 ccgggcgacc tcgggcccga cgatcgcctg aagatcctcg cgatggcgcg gctcctcgat
     3241 gcaaaggatc cgtgggcgct gctcggtgtg ccgcgtggcg ccgacgcgaa gatgctcaag
     3301 cgcgcgtact tcaagctctc gaaggacatc catcccgacc gctactacgg ccgtagcctc
     3361 ggcagcttcg cggagcgtct gacggtggtg ttcgaggcgg tgagtcgcgc gtacgcgagg
     3421 ctgacgtcgc cggagaaatc gaagggcagc ggcgcgcatg tggccgtcaa tgcagagcag
     3481 ccgcagagcc cgcaggaata tgccagcgag ctgttcgagc gcgcgtgcgg gctcgaagtc
     3541 caaggcgacg cgctcggcgc gatgaagctg ttcgcagccg tcgtccgcat cgatggtcag
     3601 acgcgctacc tccggcgagc cgcgagctgc gcgctggctg ccggtcagcc gaagaccgcg
     3661 ctcgagtacg cgaagaaggc gcagacgcag gcgccgcaag atccgtcgtc ggcgcgtctc
     3721 ctcgcgcaag catttcgcgc ggccggcaag ctcggcgacg ccgaagaagt gctcgtcatg
     3781 gcgatggcgc tgaagagcga gaacgatgtg ctgacggccg agctgcgcag cgatttggcc
     3841 gaggttcgca ggcagatcgc cgccgaaaac cgctgaaaca ctgctacccg tcagctaccg
     3901 tcgattcgag gcccggggac tggcgatctc cgcgaaattg aggggaaact agccccgcat
     3961 gcccagcgac aaggtgatcg gcatcgacct cggcacgacc aattcgtgcg tggcgattgt
     4021 cgaagatggg accccttcgg tcattccaaa ccgtggcggc tacaagacga cgccgtccat
     4081 ggtcgcgatt gccgaaaacg gtaagcggtt ggtcggccac atcgcgaagc gccaggcaat
     4141 cacgaacgcc gagaacacgg tgtacgccgc gaagcgcctc atcggtcgca agtggggctc
     4201 ggctgcagtg cgcagctcgc tcgagatcat gccgtaccga atcgtcgaag gcccgcacga
     4261 tgacgtccgc atcatgctgc gcgaccaggt cttcagcgtg cccgaggtca gcgcgtacat
     4321 cctccaggag atgaagctga tctccgagga gtacctcggc gaggaagtgc agaaggccgt
     4381 gatcacggtc cccgcgtact tcaacgacgg tcagcgccaa gcgacgaaag atgccggccg
     4441 catcgccggt ctcgacgtca ttcgcatcat caacgagccg acggcagcag cgctcgcata
     4501 cgggtttggt cgcaacatcg agcgcaaggt cgcggtctac gacctcggcg gcggcacgtt
     4561 cgatatctcg atcctcgaga tcggcaacgg cgtgttcgag gtcatctcga cggccggcga
     4621 cacgttcctc ggcggtgagg acttcgatcg ccgcatcatc gattggctcg tgttcggctt
     4681 cgagaagcag cacggcatcg atctgcgcaa agacaagatg gcgctgcagc gcctcaagga
     4741 cgtcgccgag aaggcgaagt gcgagctgtc gagcgtgaag gagacggaga tcaatctacc
     4801 gttcatcatc tcgacgggcc gcaacgaagc gcttcacctg cagacgatgc tgtcgcgcga
     4861 aaagctcgaa gagctcacga gcgacctcgt cgatcgcacc gtcgaaatct gcgctcgcac
     4921 gctcgaggaa gccgagatcg agaagaagca gatcgaggat gtcatcctcg tcggtggcat
     4981 gacgcgcatg ccgcgcgtgc agcaggccgt tgccgagttc ttcggcctcg agccgagcaa
     5041 gggcgtgcat cccgacgagg tcgtcgcact cggtgcagcg atccaaggcg cggcacttgt
     5101 tgccgacgac gagcgcagcg gcgacatcct gctcctcgac gtcacgccgc actcgctcgg
     5161 catcatgatc gtcggtggct acttccataa gctcatcgag cagaacacca ccgtgccgac
     5221 cagcgaatcg cacgtgttca cgacggtcaa agacaaccag acgtcggtga agatcctcgt
     5281 gctccaaggc gagagcgacc gcgccgaaga gaacgagctc ctcggcgagt tcgtgctgac
     5341 gggtctgcgt cgcgcgccac gtggcgaggt cgagatcgag gtccagttcc acatcagcgc
     5401 cgacggcatc gtgtccgtga aggcgaagga cctcgagacg agcctcgagc aatcgatcac
     5461 ggtgaccgcg acctcgggtc tgaccgaaga cgagatcaag aagatgatcg acgcgaacaa
     5521 ggactacatg gtcgaggttc gcaccggcca ggagttcgac aaggcgcgcc accaagccga
     5581 gaagtcgctc gaggaaatcg aagcgctgtt cccgcaggtc accgacgcga tcgccggctc
     5641 cgacttcggt caggacgccg tgaagaaagc gcgcgcggtt gtcgagcgca cgcggcaagc
     5701 gatccaagcg aaggatctga cgacgctcgc cgatgcgacc gaagcgctga accgcaccct
     5761 caacatgttc cgtggcgtgg tgtcgaagac gcgcggctag gagatcgtgc cgaagcagtc
     5821 cgatatcgat cgactcatcg agctcgggtt gaaccgctac ggtgccggcg agatcgacga
     5881 ggcgctcctc atgtgggagc aggccctcgc tgcagatccg gacaatccgc aggcaaacag
     5941 ctacgtcgat tacgttcgct ccaactacga gctgctgaca agcggtgagc ccgcgctcgt
     6001 cgacgatccg agctacgcgc tcgacgaaga gcccgactat cagatcgaga tcctgccggg
     6061 tgagctccca cggccgagcc aaccgctact gtcgcttgcc gacgaggagc tcgacgcggg
     6121 ctgggatctc gaggagaccc aggatgccgg tgggccgatg cagcgcaact acggcgacag
     6181 cttcgacgaa gacgagggcg gcacacgcga gtactacaat gcggcgaatc cgccgtccga
     6241 tttcgcggct gagtcagaga cgtcggagtt tcagcagcgg gaagtgaccg gcagctttca
     6301 gaacgagggc acgcccgtcg gttttgcgca gcaagagacg tcggtgcgac aacgcgagct
     6361 cgggttcgtg aagccgcagg gcgcgccgca gccggtgcaa gacaagccgt cgagtccgat
     6421 ggcgatcgga accgcatcga ccgtcgagct cggtgcagcg ctcgaggcag ccggcgacaa
     6481 caatccgttc gccgaggtca cgctcgaccg cagcctggag aaccagcaga cgaaggagac
     6541 agtgctgccg aagcgctcga cgcgcgacca gctcgagcaa tcgcttcgtc ctcctgcgcg
     6601 acgcacgagc gaagagctct cgcaggccga ggtcatgctg ccgcacgcac cgacgcagga
     6661 cttcgacggg cacggccgga tcgatgtcgg cgcaccgacg cgcgagctcg gattgcggcc
     6721 acccgcgcgt ctgagtaacc ccgtcgacga ggatgcaccg acgggcgagg cggacgttcg
     6781 cgcgattcga gaagcggcag ggcgtcgcga gggcaatgcg cgccccgtca gcgagggcac
     6841 gcgacacgac atcgtgctgc agttcgatcc gatcgcagca cgcagcgcgc aggtcctcgc
     6901 cgaggtagat gccgatgcgc cgcccgacga gccgaaggaa gagcagacgc gccgccgcat
     6961 cacgatgctg ttgcagaaag cgatcgagta caacgccgcg cacgaaaccg acaaggcagt
     7021 gaccgcggtc gacctcgcgc tcgccgaaga tccgaacagc gcgctcggtc agaagctcgt
     7081 cacgcgcaac aaagacacga tcatgcagat cttccagagc ttcattggtg atctcgatcg
     7141 ccagccgcac ctcgcgcggc cgctgcacga gctcgcgaaa gaaccgatca gcccgcgcgc
     7201 cgcgttcctc ctctcgcgca tcgacggcac gctcaccgtc gacgagctcc tcgatgtctc
     7261 cggcatgccc cggctcgagg cgtaccgcca cctttgccag ttgttcctgc gcggcatcct
     7321 ccggtgaacg tgctcgtcac gcgagtcacg aacatgacga ccagccggcc gcacgccggt
     7381 tcggcgctac tgcgcgtcgt cgacgggccg gaccgcggtc tcgagctcga gctgccgcac
     7441 gccggcgtcg tcctcggcac cgacaaggcg tgcgacgtcg tgctgaagga tccgttcgta
     7501 tcgcgccgcc actgctcggt cgcgccacat gcgcagggct tcgcgatcag cgatctgggc
     7561 tcgagcaacg gcacggtgat cgacggcgtc gccgtgggaa aagtcgtggc gccgccgggt
     7621 gtcgcgctgc gcatcggcaa gacgctcgtg cagctgatgc cggccgacga ggtgatcgac
     7681 atcccgccgt cgacgagcga ccgcttcggc gcgctgctcg gtgcgagcca cgtgatgcgc
     7741 caggtgttcg cgctgctcga gcgcgccagc aagtcgaacg cgccggtgct gttcctcggc
     7801 gagagcggca ccggcaagga gctgatggcg cgcggggtcc acgatgcctc gccgcgccac
     7861 gacggcccgt tcgtcgtgtt cgactgcggc gcgtcgaccg acgcgctgat cgagagcgac
     7921 ctgttcggcc acctcaaggg cgcgttcacc ggcgcggcgt cggatcgtca gggcgcgttc
     7981 gccgcggcgc acggcggcac gctgttcctc gacgagatcg gtgatctacc ggtcgcgctg
     8041 cagccgaagc tcctgcgcat gctcgaggcc ggcgaagttg tcccgcttgg cgggcgcaag
     8101 tccgagcgct acgacgtgcg catc
//
LOCUS       DATLBZ010000264         9443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7594606, whole genome shotgun sequence.
ACCESSION   DATLBZ010000264 DATLBZ010000000
VERSION     DATLBZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7594606"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VMZ53_13910"
     CDS             <1..277
                     /locus_tag="VMZ53_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29602.1"
                     /translation="SHGMADVAGINKVLEGRPHCVDAMDNHEIDFVINTTEGAQAIHD
                     SQSLRRAALMNGIAYYTTLRAAKAALEAIAVEKRNDMRVQALQIYAR"
     gene            complement(274..870)
                     /locus_tag="VMZ53_13915"
     CDS             complement(274..870)
                     /locus_tag="VMZ53_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29603.1"
                     /translation="MRSAVCLAGILAICALAHAQGSPDAGVPSPDAGLAAPSVAVDAG
                     VAPMIDAPPAPMAIDAAAAGPVDAAGQADAAADVSLALPPPSRSQDAAWLLVGGTLTF
                     ITTGIVLAYAAESTETDIEDLYVSLNGQSPTFDAKTQERYDELVKQGNRYQYLSWISF
                     GIAGGMAIGATVLFWKHHKEPAVVPVVTPNSAGVSVRF"
     gene            930..2966
                     /gene="fusA"
                     /locus_tag="VMZ53_13920"
     CDS             930..2966
                     /gene="fusA"
                     /locus_tag="VMZ53_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HUS29604.1"
                     /translation="MAKIDMVRNLGVVAHIDAGKTTVSERFLFHSGKIHKVGEVHDGE
                     TEMDWMEQERERGITITAAATTFEWKKHEIHLIDTPGHVDFTIEVERSLRVLDGAVVV
                     FCGVAGVQPQSETVWHQADKFKVPRLAFVNKLDRIGASFSAVVADIKERLGANAVPIQ
                     LPIGTEDAFTGCVDLVAMKAYMFSGDVTETPEPVDIPADMAATAADARDQMIQALADL
                     DDGIAAKYLEGQDLTEAEIKAVIRKSTINVKMIPVLAGTALRNKGIHPLLDAVIDYLP
                     SPADLPPVAGVDPRDTNEKLQRAAKNNEPFAALAFKIAMDEGRKVVFMRIFSGAVEAG
                     GEVLNVRTNKKEKIARLFRIHAAKRERLDKAFAGEIVAAAGLKDATTGDTICDPKSPI
                     LLERIDTYEPVISQAIEAENQAAKERLDFALGKMGEEDPTFRVKDDPDTGQTIISGMG
                     ELHLEIIVDRMKREYGVNARAGKPQVVYRETVLKSGEGTAEFERDLKEQAIFGSATCK
                     VQGRARGSGMEFKKSLPASAALLPPHIVEAGMQGLRDAASSGPDGYPLEDVEVTLTNL
                     VIREGANGEIGCRAAAAEAFRRAVQGATPSRLEPIMAVEVTTDDEFLGGVIGDLNQRR
                     GHVQDVSSRGAKRVVTAHVPLRNMFGYSTRLRSLSEGRATFSMQFQAYDTLQSA"
     gene            complement(2963..4300)
                     /locus_tag="VMZ53_13925"
     CDS             complement(2963..4300)
                     /locus_tag="VMZ53_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29605.1"
                     /translation="MRWALFAIALAACNGGQGVDIEVHSDGKSIDAVELWLAYGICKL
                     PDGSDCDGIAWPGAKARPPGTVFTLPENDERVFRTETLVDGVAQIHLETVPGAEAPFA
                     MAVVGFHGTDVVGVKLISPVEIPTDDQARWVVKLREANDATDDITKDPGEGSKFNARV
                     WARKPSSTLPDATGYTGCLAYQHWTGSEWESLYFVPNSDPDCDGDPPDCNAYWYDAPV
                     GSAKCVGEPVTVANVCAVGNVSCRAETDQIGCDVQAPLVCVPSALCNTCADTPDLVAC
                     LKAQVGTSSGIAMMPAQKCSFVQADAAPCANSTNPVGWTARFTIRGATCATAGGEIAV
                     LRPMSAPFSGGGATLPVNGAGMLVHAATSGGNCLVDVTQIDGPSLNAGPVPVIVAIHF
                     GTAREILIPVLLDFQTVATMNNCPIATPVNCMQIGQWPETTVPGDSMFRCAVP"
     gene            complement(4313..5629)
                     /locus_tag="VMZ53_13930"
     CDS             complement(4313..5629)
                     /locus_tag="VMZ53_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29606.1"
                     /translation="MRAFSIGVVMMLASCGSSGGVQLLVTPTPDVERVALYVGVGDED
                     RQGLAPPGFGSPISTSTWVRDDHNELSEVMNVGGNQVSFGLLGPGDDLSIVIAVGFDG
                     GGKAVAAAVLPKELRPLAIPSSYVARYELTLAPITAFDSASKLDVRVWKPIDPDAPGE
                     KAECVFAADGRTGERVAVVAGNDADCDAWPTEPRDKECLPAVFMGTKLPKLDASTSCL
                     KAIVANDPNASVAAPAAVLGGKKCVDGQGSIDGCFESNYCIPKSVLDSCMSFGDFTCA
                     QDITAHQINVPTHMECYIHNDGNGKLCADPIPALSDPRQLIDVHSCKASSARISRPGQ
                     VWSNKVTFADPTNGTAFDITVANPTACDITLTPSGTLLAGTVDTERRGALVAGDLDNG
                     RGLAMPYVFIIDPLKRGCGSPTPCTLAYGNGSIADGTFQCMNTAPL"
     gene            5752..6609
                     /locus_tag="VMZ53_13935"
     CDS             5752..6609
                     /locus_tag="VMZ53_13935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HUS29607.1"
                     /translation="MARAVRAAPHVQEVVASPVVKWVGGKTKLLPELLSRMPERFNRY
                     YEPFAGGAALFFRLAPKRAVLADSNHDLVGLYTAIAKDVNAVIRRLEMHREAHDESHY
                     YETRERWNDREVSWSTPERAAAFIYLNKTCFNGLWRVNRSGAFNVPIGRYTDPPICVP
                     DTLRAAQQVLARAEIRRADYREAVHDAKAGDFLYFDPPYDPVTTTANFTAYTANVFGA
                     DEQRMLADTARQLVAKGCYVMLSNSDTPFMRSIYKGFRVDRVKCARAINSNAAKRGDV
                     DELVITGGY"
     gene            complement(6619..6951)
                     /locus_tag="VMZ53_13940"
     CDS             complement(6619..6951)
                     /locus_tag="VMZ53_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29608.1"
                     /translation="MIQTTTSQTLDGLWVLLVSNDGELQAQDANGRVPVMCRAGNDQT
                     YLLVFKNVVKARQFLSAQSIDDAEPRMVVRGNRDDIVRIAKSAGVVGTMLDYDPSTQK
                     YASAAALA"
     gene            7043..8089
                     /gene="moaA"
                     /locus_tag="VMZ53_13945"
     CDS             7043..8089
                     /gene="moaA"
                     /locus_tag="VMZ53_13945"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HUS29609.1"
                     /translation="MSRLHLPVLGKPVLPAVAPADPLLDGFARRIRYLRISVTDRCNY
                     RCTYCMPETLGKEIEFQPRSAVLTFEELEQIATVFASLGVRKIRLTGGEPTVRHGIVE
                     LVRRVAAVPGIEQVVMTTNGHLLAELAVPLRDAGLSAVNVSVDTLDADRFRAITSRGD
                     LAKVLAGIDAAVAAGLRVKTNAVALEGINDTELVALVEDAWRRGAIPRFIEHMPMSDG
                     VLYEAARGITAATIRRTIEAQLGPLTRADAGGADAGPARYWALADGRQVGIISAMTEH
                     FCDDCNRLRLTATGELHACLGHDDAVSLRDVLRNGGLREDLVRAIAGAVTGKRAGHLF
                     DSNAPQKHMIGIGG"
     gene            8179..8694
                     /locus_tag="VMZ53_13950"
     CDS             8179..8694
                     /locus_tag="VMZ53_13950"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HUS29610.1"
                     /translation="MDTRLLPKIALTLGIVVGGGGFLVYSSRSHAQHYEMVDKLVDKG
                     IDNYKGKELKVHGYVEAGSIVESIVNQEQRRTFVLQKAGKKIRVFSVGPKPDTFKDQS
                     EVVATGRLIASADVQKLADEICASAKGNPSCPIRTDAEQHMVVESSELMAKCPSKYEG
                     AQSNKLDTNFK"
     gene            8701..>9443
                     /gene="ccsA"
                     /locus_tag="VMZ53_13955"
     CDS             8701..>9443
                     /gene="ccsA"
                     /locus_tag="VMZ53_13955"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HUS29611.1"
                     /translation="MTDQGVPFSPIALVGTISLLLAYLVAAWCVASGIAGNARKSRRL
                     VTSSVYGLYGFAALVSLSAALIIYAFVTHDFSIKYVAMTSKTSMPTAYKITAFWGGLD
                     GSLLFWVLVLALFSTIAIATNHRRHRDMIGYVVATIMVVQVFFLSLLIFSKNPFSTYL
                     TTPPVDGKGLNPLLQNYWMVIHPPSLYTGFVAATIPFAFGIGALASGRLDDKWLGSVR
                     VWVLVCFGFLSLGLILGGRWAYEELGWGG"
BASE COUNT         1690 a         3073 c         3035 g         1645 t
ORIGIN      
        1 gagccacggc atggccgacg tcgccggcat caacaaggtg ctcgaaggcc gcccgcactg
       61 cgtcgacgcg atggacaacc acgagatcga tttcgtcatc aacacgacgg aaggcgcgca
      121 agcgatccac gattcgcagt cactgcgccg cgccgcgctg atgaacggca tcgcgtacta
      181 cacgacgctg cgtgccgcga aggccgcgct cgaagcgatc gccgtcgaga agcgcaacga
      241 catgcgcgtg caagcgctgc agatctacgc gcgctagaag cgaacgctga cgcctgcgct
      301 gttcggcgtt acgaccggca cgaccgccgg ctctttgtgg tgcttccaga acagcaccgt
      361 cgcaccgatc gccatgccgc ccgcgatgcc gaacgagatc cacgacaggt actggtagcg
      421 gttgccctgc ttgacgagct cgtcgtaccg ctcctgcgtc tttgcatcga acgtcggtga
      481 ctggccgttg aggctcacgt agagatcctc gatgtccgtc tccgtcgact cggcggcgta
      541 cgcgagcacg atgcccgtcg tgatgaacgt gagtgtcccg ccgacgagca gccacgcggc
      601 gtcctgactg cgcgaagggg gagggagggc gagcgagacg tcggctgcag cgtcggcttg
      661 ccctgccgca tcgaccgggc cggcagcggc tgcatcgatg gccatcggcg cgggcggtgc
      721 gtcgatcatg ggcgcgacgc ctgcgtccac ggcgacggaa ggcgccgcta aacctgcatc
      781 gggtgagggc acacccgcat cgggagaacc ttgtgcgtgc gcgagcgcgc agatcgcgag
      841 aatcccggcg aggcagacgg ctgaccgcac gaccggagcg tacagatccg gcgagagatg
      901 cgctataccg cgtcccggag taaccccgca tggccaagat cgatatggtc cgcaacctcg
      961 gtgtggtcgc gcacatcgac gcaggcaaga cgaccgtctc ggagcggttc ttgttccatt
     1021 cgggaaagat ccacaaggtc ggcgaggtcc acgacggtga gaccgagatg gactggatgg
     1081 agcaggagcg cgagcgcggc atcacgatca ccgcggccgc gaccacgttc gagtggaaga
     1141 agcacgagat tcatctcatc gatacgcccg gacacgtcga cttcacgatc gaggtcgagc
     1201 gttcgttgcg cgtgctcgac ggcgcggtcg tcgtgttctg cggcgtcgcc ggcgtgcagc
     1261 cgcaatcgga gacggtttgg caccaggccg ataagttcaa agttccgcgg ttggcgttcg
     1321 tcaacaagct cgatcgaatc ggcgcgtcgt tcagcgccgt cgtcgctgac atcaaggagc
     1381 ggctcggcgc gaacgccgtg cccattcagc tgccgatcgg caccgaggat gcgtttaccg
     1441 gctgcgtcga tctcgtcgcg atgaaggcgt acatgttctc gggcgatgtg accgagacgc
     1501 ccgagcccgt cgacatcccg gccgacatgg ccgcaacggc cgcggatgcg cgcgatcaga
     1561 tgattcaagc gctcgccgat ctcgacgatg ggatcgcggc gaagtacctc gagggccaag
     1621 acctgaccga ggcggagatc aaggcggtca tccgcaagtc gacgatcaac gtcaagatga
     1681 tccccgtgct cgccggtacg gcgctgcgca acaagggcat ccatccgctg ctcgacgcgg
     1741 tgatcgacta tttgccgtcg ccggctgatt tgccgcctgt ggcgggcgtc gatccgcgtg
     1801 acacgaacga gaagcttcag cgcgcggcga agaacaacga gccgtttgcg gcgctcgcgt
     1861 tcaagatcgc gatggacgag ggccgcaagg tcgtcttcat gcgcatcttc agcggcgcgg
     1921 tcgaggcggg cggcgaggtg ctcaacgtcc gcacgaacaa gaaagagaag atcgcgcggc
     1981 tcttccgcat tcatgcggcg aagcgcgagc gcctcgacaa ggcatttgcc ggcgagatcg
     2041 ttgcggctgc gggcctcaag gacgcgacga cgggcgacac gatctgcgat ccgaagtcgc
     2101 cgatcctcct cgagcgcatc gatacctacg agccggtgat ctcgcaggcg atcgaggcgg
     2161 agaaccaggc ggcgaaggag cgcctcgact tcgcgctcgg caagatgggc gaagaggatc
     2221 cgacgttccg cgtgaaggac gatccggata ccggacagac catcatctcg ggcatgggcg
     2281 agctccacct cgagatcatc gtcgaccgca tgaagcgcga gtacggcgtg aatgcgcgcg
     2341 ccggtaagcc gcaggtcgtc tatcgcgaga cggtgctgaa gtcgggcgag ggcaccgcgg
     2401 agttcgagcg cgatctcaag gagcaggcga tctttggatc cgcgacgtgc aaagtgcagg
     2461 gcagggcgcg cggctccggc atggagttca agaagtcgct gcctgcctcc gcggcgctgc
     2521 ttccgcctca catcgtcgag gctggaatgc agggacttcg cgacgccgcg tcgagcggtc
     2581 ccgacggcta tccactcgag gacgtcgagg tcacgctgac gaacctcgtg atccgagaag
     2641 gcgcgaacgg cgagattggc tgtcgcgctg ccgccgcgga agcgttccgc cgcgcggtcc
     2701 aaggtgccac gccgtcacgg ctcgagccga tcatggccgt cgaggtcacg accgacgacg
     2761 agttcctcgg cggtgtcatc ggtgatctca atcagcggcg tggccacgtt caggacgtca
     2821 gctcgcgcgg cgccaagcgc gtcgttacag ctcacgttcc gctgcgcaac atgttcggct
     2881 actcgacgcg gcttcgcagc ctcagcgaag gtcgcgcgac gttctcgatg cagttccagg
     2941 cctacgacac gctgcagtcg gcttagggca ctgcgcagcg gaacatcgag tcgccgggga
     3001 ccgtggtctc cggccactgt ccgatctgca tgcagttcac tggcgtcgcg atcgggcagt
     3061 tgttcatcgt cgccaccgtc tgaaaatcga gaaggacggg aatcaggatc tctctcgcgg
     3121 ttccgaagtg gatggcgacg atgacgggaa ccggccctgc attcagcgat gggccatcga
     3181 tctgcgtgac atcgacgagg cagtttccgc ccgaggtggc cgcatgaacg agcatgcctg
     3241 caccgttgac gggtagcgtc gcgccgccgc cggagaacgg tgcactcatc gggcggagca
     3301 ccgcgatctc gccgccggct gtggcacacg tcgctccgcg aattgtgaat cgcgcggtcc
     3361 agccgacggg gtttgtcgag ttcgcgcacg gagctgcgtc tgcttgaacg aacgagcact
     3421 tctgcgctgg catcatcgcg atgcccgacg acgtacccac ttgcgccttc agacacgcga
     3481 caagatctgg cgtgtccgca catgtgttgc agagcgcgct tggaacgcag acgagcggtg
     3541 cctgcacgtc gcagccgatt tgatcggtct cggcgcgaca cgagacattg cccaccgcgc
     3601 agacgtttgc gaccgttacc ggctccccaa cgcacttcgc gctgccgacg ggagcgtcgt
     3661 accaatacgc attgcagtcg ggcggatcgc cgtcgcagtc cggatctgaa ttcgggacga
     3721 agtagagcga ctcccactcg ctgccggtcc aatgctgata cgcgaggcag ccggtgtagc
     3781 cagtcgcgtc gggtaacgtg gacgatggct ttcgcgccca cacccgcgcg ttgaacttgg
     3841 agccttctcc tgggtccttc gtgatgtcgt cggtggcgtc gttcgcttcg cgcagcttca
     3901 ccacccagcg cgcttgatcg tcggtcggaa tctcgacggg actgatcagc ttcacgccga
     3961 cgacgtccgt tccgtggaag ccgacgaccg ccatcgcgaa cggtgcttcg gcgcccggca
     4021 ccgtctcgag atgaatctgc gcgacgccgt cgacgagcgt ttcggtgcga aacaccctct
     4081 cgtcattctc gggcaacgtg aacaccgtcc ccggcggtcg ggccttcgcg cccggccacg
     4141 cgatgccgtc gcagtcgctg ccatcgggaa gcttgcagat gccgtaggcg agccacagct
     4201 cgacggcatc gatcgatttg ccatcgctat gcacttcgat gtcgacaccc tgaccgccat
     4261 tgcatgcggc caacgcgatc gcgaacagtg cccagcgcac ggcttcagcg tatcagagcg
     4321 gcgcggtgtt catgcactgg aacgtgccgt cggcaatgga cccgttgccg taggccaagg
     4381 tgcacggcgt cggggaccca catccgcgct tcagcggatc gatgatgaag acgtacggca
     4441 tcgcgaggcc gcggccattg tcgaggtcgc cggcgacgag tgcgccgcgg cgctcggtgt
     4501 cgacggtgcc agccaatagg gttcccgacg gagtgagcgt gatgtcgcac gccgtgggat
     4561 ttgccacagt gatatcgaac gcggtcccgt tggtcgggtc ggcgaacgtc accttgttcg
     4621 accagacttg tcccggccgc gagatccgcg ccgagctcgc cttgcacgaa tgcacgtcga
     4681 tcaactgccg ggggtccgac aaggccggaa tcggatcggc gcacagcttt ccattgccgt
     4741 cgttgtggat gtagcactcc atgtgcgtcg ggacgttgat ctgatgcgcc gtgatgtcct
     4801 gcgcgcaggt gaagtcgccg aagctcatgc acgagtcgag cacgctcttc ggaatgcagt
     4861 agttgctctc gaagcaacca tcgatcgagc cctggccatc gacgcacttc ttgccgccga
     4921 gcaccgccgc cggtgctgca acgctggcgt tcggatcgtt cgctacgatc gccttgaggc
     4981 acgacgtcga tgcgtcgagc ttcggcagct tcgtgcccat gaagactgcc ggcaagcatt
     5041 ccttgtcgcg cggctccgtc ggccacgcat cgcagtcagc gtcattaccg gcgacgaccg
     5101 cgactctctc gccggtcctg ccatcggccg cgaacacgca ctccgccttc tcgcccggcg
     5161 cgtccggatc gatcggcttc cacacacgga cgtcgagctt cgatgctgaa tcgaacgccg
     5221 tgatcggagc cagcgtgagc tcgtagcgcg cgacgtacga ggagggaatg gcgagcggtc
     5281 gcagctcctt tggcagcact gcagcagcaa cggctttgcc accgccatcg aagccgaccg
     5341 cgatcacgat cgacaagtcg tcgcccggac cgagcaatcc gaacgagacc tgattgccgc
     5401 cgacgttcat cacttcggac agctcgttgt gatcgtcgcg cacccacgtc gacgttgaaa
     5461 tcggcgaccc gaacccgggt ggcgcgagac cttgtctgtc ttcgtcaccg acgccgacgt
     5521 agagcgcgac gcgttcgaca tccggtgttg gcgtgacgag cagctgcacg ccgcccgacg
     5581 agccacagct cgcgagcatc atcaccacgc cgatcgaaaa cgctctcact cgatcacgat
     5641 acatcgcgct gcttatcaaa gcgcaattat cgaagtgacg tagcgctcgt tcgaaaattg
     5701 gcccgcgatc ttcgcgccct cgacgatact tttatggctc atcgctcgag ggtggcgcgt
     5761 gctgtacgcg ctgctccgca cgtccaggaa gttgtcgcgt ctccggtggt caagtgggtc
     5821 ggtggcaaga cgaagctatt gcccgagctg ctctcgcgga tgcccgagcg attcaatcgc
     5881 tactacgagc cgttcgccgg cggcgcagcg ctgttcttcc ggctcgcgcc caagcgcgcg
     5941 gtgctcgcgg attccaacca cgatctcgtc ggcctctaca cggcgatcgc gaaagacgtg
     6001 aacgcggtca tccgccgcct cgagatgcat cgcgaagcgc acgacgagtc gcactattac
     6061 gagacgcgcg agcggtggaa cgatcgcgaa gtgtcgtggt cgacgccgga gcgcgcggct
     6121 gcgttcatct atctcaacaa gacgtgcttc aacgggctct ggcgtgtgaa tcgctcgggt
     6181 gcgttcaacg tgccgatcgg tcgctacact gatccgccga tttgcgtgcc cgataccctg
     6241 cgcgctgcgc agcaagtcct cgcgcgcgcc gagattcgcc gcgccgacta tcgcgaagcc
     6301 gtgcacgacg cgaaggcggg cgacttcctc tacttcgatc cgccgtacga cccggtgacg
     6361 acgaccgcga acttcacggc gtacaccgcc aacgtgttcg gcgcagacga gcaacgcatg
     6421 ctcgcggaca ccgcgcgcca gctcgtggcg aagggctgct acgtgatgct gtcgaacagc
     6481 gacacgccgt tcatgcgctc gatctacaaa ggcttccgcg tcgaccgcgt gaagtgcgcg
     6541 cgcgccatca actcgaacgc cgccaagcgt ggcgacgtcg acgagctcgt aattaccggc
     6601 ggctattagc cgcgacggct acgcgagcgc agcggcggac gcgtacttct gcgtgctggg
     6661 gtcgtagtcg agcatcgtgc cgaccacacc agcagatttc gcaatccgca cgatgtcgtc
     6721 gcggttgccg cgaaccacca tgcggggctc ggcgtcgtcg atcgactgcg ccgagagaaa
     6781 ctgccgtgct ttcacgacat tcttgaacac cagaaggtac gtctggtcgt tgcctgcgcg
     6841 gcacattacg ggaacgcggc cgttggcgtc ttgcgcctga agctcaccgt cgttcgacac
     6901 cagcaagacc cacagtccgt cgagggtctg ggaggtcgtc gtttggatca tatttaggtg
     6961 cgagatacat gcggtgtttc ggcggatctg tcaaggatct ttgttacctg cggaggccgc
     7021 tgacgtatgc tccgctgacg tcatgagtcg gctccatcta ccggtactcg gcaagcccgt
     7081 gctgccagcg gtagcccccg ccgacccgct cctggacggc tttgccaggc gcatccgata
     7141 cctgcgcatc agcgtgacag accgctgcaa ctaccgctgc acgtactgca tgcccgagac
     7201 gctcggcaag gagatcgaat tccagccccg ctcggcggtc ctcacgttcg aagagctcga
     7261 gcagatcgcc accgtgtttg cgtcgctcgg cgtgcgcaag attcgcctga ccggcggtga
     7321 gccgacggtt cgccacggca tcgtcgagct cgtacgccgc gtcgcggcgg tgcccggcat
     7381 cgagcaggtc gtcatgacga cgaacggcca tctgctcgcc gagctcgcag ttccgctgcg
     7441 cgatgcgggc ctctccgccg tgaacgtcag cgtcgatacg ctcgacgcag atcgcttccg
     7501 cgcgatcacg agtcgcggcg acctcgcgaa ggtcctcgcc ggcatcgatg cggcggtcgc
     7561 ggcaggcctg cgcgtcaaga cgaacgccgt cgcgctcgag ggcatcaacg acaccgagct
     7621 cgtcgcgctg gtcgaagatg cgtggcggcg tggcgccatc ccgaggttca tcgagcacat
     7681 gccgatgagc gacggcgtcc tgtacgaggc cgcacgcggg atcaccgccg cgactattcg
     7741 ccgcacgatc gaagcgcagc tcggtccgct gacgcgcgcc gacgcgggcg gagccgacgc
     7801 aggtcctgcg cgttactggg cgctcgcaga cggccgccag gtcggaatca tctcggcgat
     7861 gaccgaacat ttctgcgacg actgcaaccg gctgcgcctc accgcgacgg gcgagctgca
     7921 tgcgtgcctg gggcacgatg acgcagtcag tcttcgcgac gtgctgcgca acggcggctt
     7981 gcgcgaggac cttgtccggg cgatcgcagg ggctgttacg ggcaaacgcg cgggacatct
     8041 tttcgactcc aatgccccgc aaaagcacat gatcggcatc gggggttaac gagccggaat
     8101 ttgcctggcc atgctacggt tgcccccggg taacgccaac gggacgccgg gcctgtcgca
     8161 cgagcgaggc acccccatat ggacacacga ctccttccaa agatcgcgct gaccctcggc
     8221 attgtcgtcg gtggcggcgg ctttctcgtc tactcgtcgc gtagccacgc gcagcactac
     8281 gagatggtcg acaagctcgt cgacaagggc atcgacaact acaagggcaa ggagctcaag
     8341 gtccacggtt acgtcgaggc cggatcgatc gtcgaatcga tcgtgaacca ggagcagcgc
     8401 cgcacgttcg tgctgcaaaa ggccggcaag aagatccgcg tgttcagcgt gggtccaaag
     8461 cccgacacgt tcaaagatca atccgaggtc gtcgcgaccg gacggctgat cgcatcggcc
     8521 gacgtgcaga agctcgccga tgaaatctgc gcctccgcga agggcaaccc gagctgtccg
     8581 attcgaaccg acgccgagca gcacatggtc gtcgagtcga gcgagctaat ggcgaagtgc
     8641 ccgtcgaagt acgaaggcgc tcaatcgaac aagctcgaca cgaacttcaa gtgaacccta
     8701 atgactgacc aaggggttcc gttctcaccg atcgcgctgg tcggtacgat ttctctcctg
     8761 ctcgcgtatc tcgtcgccgc atggtgcgtg gcgtcgggca tcgcaggcaa tgcgcgcaag
     8821 agccgtcgcc tcgtcacgtc gagcgtgtac ggcctctacg gcttcgccgc gctcgtgagc
     8881 ctgtcggccg cgctcatcat ctacgcgttc gtcacgcacg acttctcgat caagtacgtc
     8941 gcgatgacga gcaagacgtc gatgccgacg gcgtacaaga tcaccgcgtt ctggggcggc
     9001 ctcgatggct cgctcttgtt ctgggtgctc gtgctcgcgc tgttctcgac gatcgcgatc
     9061 gcgacaaacc accggcgaca tcgcgacatg atcggttacg tcgtcgcgac gatcatggtc
     9121 gtgcaggtgt tcttcctgtc gctcctgatc ttctcgaaga acccgttctc gacgtacctg
     9181 acgacgccgc ccgtcgacgg caagggcctc aatccgcttc tccagaacta ctggatggtc
     9241 atccacccgc cgagcctcta cacgggcttc gtcgcggcga cgatcccgtt cgcattcggc
     9301 atcggcgcgc tcgcgtccgg ccgcctcgac gacaagtggc tcggctcggt gcgcgtctgg
     9361 gtgctcgtct gcttcggctt cctctcgctc ggtctcatcc tcggcggtcg ctgggcctac
     9421 gaggagctcg gctggggcgg cta
//
LOCUS       DATLBZ010000265         2733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10581491, whole genome shotgun sequence.
ACCESSION   DATLBZ010000265 DATLBZ010000000
VERSION     DATLBZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10581491"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1793
                     /gene="istA"
                     /locus_tag="VMZ53_13960"
     CDS             195..1793
                     /gene="istA"
                     /locus_tag="VMZ53_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HUS29612.1"
                     /translation="MANVLSDEKRQQVEALGRLGWTLRRIQRETGVRRETASDYLRAA
                     GIAIRSRGGAPPKPATSAEVITDPNSKPATSVEVITDSGNSKPATSAVVITDSTPRSA
                     SSCEPYRELIERFVGDGRHAMSIWQELIEKHGFTARYAAVQRFVRGLRETQPPEARVV
                     IATTPGEEAQVDYGDGPMVRYAATGKYRRTRLFLMTLAHSRKAVRLLTWQSSSKIWAQ
                     LHEQSFRRLGGATATVVLDNLREGVITPDVYDPLLNPLYREVLAHYGAVGLPCRVRDP
                     DRKGKVESGIAHTQKALRGLRFESLEEAQKWLDNWDARWADTRIHGTTKRQVAAMFAD
                     ERPHLRSLPLEPFRYYSFGERAVHLDGFVEVLGAYYAPPPGWIGRSVRVQWDEQHVRL
                     LDPTSGALLREHLVADRGRHRIRAEDQPSRTPPDVAVLFMRAHGIGPATGAIADAEYA
                     TNRVHGIRRTLGLCSLAKKHGATAVERAANVALDAGALTYRAVKACLVHHQAAPLALR
                     QVDPLIRGLTEYRDLVCRITGEPS"
     gene            1790..2551
                     /gene="istB"
                     /locus_tag="VMZ53_13965"
     CDS             1790..2551
                     /gene="istB"
                     /locus_tag="VMZ53_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HUS29613.1"
                     /translation="MNIVEIDRALRRLRLSGMAEVLEVRIQGAAAERQPHLDFLSALV
                     GDELLRRQDRLLGRRVKQAGFRDTGKTLDTFDLDFNKKMNRALMYELATAHFVEKRED
                     VLFLGAPGTGKSHLAQAIAHSAIQQGHRVRYREAHTLLEELADASLERRRKEVMTDLA
                     TVPLLVIDDLGMRKLPTTAAEDLLELIMRRYERASTLITSNRPVDDWGKLLGDTAAVT
                     ALLDRLLHHAHVLQCGPRSWRTHHAASLPKDSNSK"
BASE COUNT          501 a          928 c          874 g          430 t
ORIGIN      
        1 tctgtcaaca ggtcgccaac ggagtgaagc atctcgtgtt gagcgacccg cgtttgcagg
       61 ggttcgacgt aacgaagaat gttgcctcgg tgatcaccca aaaccggcca ctgaagatca
      121 cctgaaaacc ggccactgaa ggccgctcgc gggacgtcga caaagttgtc ggacctctgt
      181 ggtccgccga cggaatggcg aacgtcttga gcgacgagaa gcgacagcag gtagaagcac
      241 tgggccgact cggctggacg ctgcggcgga tccagcgcga gacgggcgtg cgtcgcgaga
      301 ccgccagcga ttacctccgc gcggccggca tcgccattcg cagtcgcggt ggcgcgccac
      361 caaaaccggc cacttccgcc gaggtgatca ccgaccccaa ctcaaaaccg gccacttcgg
      421 tcgaggtgat caccgactcc ggcaactcaa aaccggccac ttcggccgtg gtgatcaccg
      481 actcgacccc gcggtcggcg agttcgtgcg agccgtaccg cgaactgatc gagcgctttg
      541 ttggggacgg acggcacgcg atgtcgatct ggcaggagct gatcgagaag cacggtttca
      601 ccgcgcgcta cgccgcggtc cagcgcttcg tgcgaggtct acgagagacg cagccacccg
      661 aggcccgcgt ggtgatcgcc acgacgcccg gcgaggaggc gcaggtcgac tacggcgacg
      721 gaccgatggt ccgctacgcg gcaaccggca agtaccgacg cacgcggctc ttcctgatga
      781 cgctcgcgca cagtcgcaag gcggtgcggc tgctgacgtg gcagtcgtcg tcgaagatct
      841 gggcgcagct gcacgagcaa tcgtttcgtc gtctcggtgg tgcgactgcg acggtcgtgc
      901 tcgacaacct gcgcgagggc gtgatcacgc ctgacgtcta cgatccgctg ctcaacccgc
      961 tctaccgcga ggtgctcgcg cactacggcg cggttgggct gccgtgccgc gtccgcgatc
     1021 cggatcgcaa gggcaaggtc gagtccggca ttgcgcacac gcagaaggcg ctgcgcgggc
     1081 ttcgcttcga atccctcgag gaagcgcaga agtggctcga taattgggat gcgcgctggg
     1141 ccgacacgcg gatccacggc acgacgaagc gccaggtcgc ggcgatgttt gccgacgagc
     1201 ggccgcatct gcgctcgctt ccgctcgaac cgtttcgcta ctattccttc ggcgagcgcg
     1261 cggtgcacct cgatggtttc gtcgaggtcc tcggcgcgta ctacgcgcca ccgccgggct
     1321 ggatcggtcg cagcgtgcgg gtgcaatggg acgagcagca cgtgcgcctg ctcgatccga
     1381 cgagtggcgc gctgcttcgc gagcatctcg tcgccgatcg tggccgccac cggatccgcg
     1441 ccgaggatca gccatcgcgc acgccgccgg atgtcgcagt cctcttcatg cgtgcacacg
     1501 gcatcggtcc ggccaccggc gcgatcgccg acgccgagta cgcgaccaat cgagtgcacg
     1561 gcatccgtcg cacgctcggg ctgtgctcgc tcgcgaagaa gcacggcgcg actgctgtcg
     1621 agcgagcggc gaacgtcgcg ctcgacgccg gcgcgctcac gtatcgcgcc gtcaaggcgt
     1681 gtcttgtcca ccaccaggcg gcaccgctcg cactccgcca ggtcgatcca ctgatccgcg
     1741 ggctgactga atatcgcgac ctcgtctgtc gcatcaccgg agaaccatcg tgaacatcgt
     1801 agaaatcgat cgcgcgctgc gcaggctgcg tctctcgggc atggccgagg tgctcgaagt
     1861 ccgaatccaa ggcgccgcgg cagagcgcca accgcacctc gactttctct ccgcgctcgt
     1921 cggtgacgag ctgctgcgtc gacaagatcg cttgctcggc cgacgcgtca agcaggcggg
     1981 cttccgcgac accggcaaga ccctcgacac cttcgacctc gacttcaaca agaagatgaa
     2041 tcgcgcgctg atgtacgagc tcgccaccgc tcactttgtc gagaagcgag aggacgtgct
     2101 ctttctcggt gcgccgggca cgggcaagag ccacctcgca caggccatcg cgcacagcgc
     2161 gatccagcaa gggcatcgtg tccgctaccg cgaggcgcac acgctgctcg aagagctcgc
     2221 cgatgcgagc ctcgagcgtc ggcgcaagga ggtcatgacc gatctcgcga ccgtgccgct
     2281 actcgtgatc gacgacctcg ggatgcgcaa gctcccgacc accgcggccg aggacctgct
     2341 cgagctgatc atgcgccgct acgagcgcgc ctcgacgctc atcacctcga accgcccggt
     2401 cgacgactgg ggcaagctgc tcggcgacac agccgctgtc accgcgctgc tcgaccggct
     2461 cctccaccat gcccacgtgc tccagtgcgg acctcgcagc tggcgaactc atcacgcggc
     2521 gagcttgccg aaggactcca actcgaagta aggaacaccg cgtcccaaca acgacacgaa
     2581 gtggccggtt ttcagacgat cacaagtggc cggttttgag gtgatcgccg aggcaaatgc
     2641 gcagcgaaat cctgcgaagg cacgaggcct cggcatgagc ccggctatat tccgacccga
     2701 tgactccgat tgcgcaagca ttagcgagag tac
//
LOCUS       DATLBZ010000266        11374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4010375, whole genome shotgun sequence.
ACCESSION   DATLBZ010000266 DATLBZ010000000
VERSION     DATLBZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4010375"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VMZ53_13970"
     CDS             <1..1054
                     /locus_tag="VMZ53_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS29614.1"
                     /translation="PDGMRVAGVVENSMAMRAGLLPGDIVTSIAKQPTRNLCELSHAL
                     RVAGRAPTTQLIFVRGADLYVREVEAIELARDPNVTYGEHRGLRTLATQPPNARALIV
                     FLQGIACESVDAPLADTPLEALIQSWTAAGYATLRFDKRGIGDSDGGPCREIDFATEL
                     ADARAIVEHAASRGLPLIIFGHSVGGIMAPLLAKHASALIVYGTPVMPWLECLQDSVR
                     RQLELRHVGEHTIVEQVAQLARLADDGELNGRSAAYHRQLAELDIAAAWRTVDAPVLV
                     VRGEHDWVVRPDDQARIAELARGAVTIVDVPALDHLFGAHADRDASLRDYGIGAARGG
                     DSLARVTLDWLARGVA"
     gene            1060..1524
                     /locus_tag="VMZ53_13975"
     CDS             1060..1524
                     /locus_tag="VMZ53_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29615.1"
                     /translation="MELSPCPACKRHVRIAAASCPFCATRLPVLQARRAIPRSLTRAA
                     IFSAALAGCQTKQSAPAPSAGSAAPIVAPPAPVADAADMPITGSNAGSAVVADDASVA
                     ADAAAAGSGVGSALRQEKLHRLKEKRTQHIKIPDERQMAKPYGAPPARRRVV"
     gene            complement(1521..2735)
                     /locus_tag="VMZ53_13980"
     CDS             complement(1521..2735)
                     /locus_tag="VMZ53_13980"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUS29616.1"
                     /translation="MKKLPLAPKAPPARRNLPLAEQVREIDVAARPILAVWEITLACD
                     LACGHCGSRAGKARPDELSTAEALSLVDQLAELGVIEVVLIGGESYLREDWCEIIARI
                     AERGMEPLLTTGGRNMTLERAHAAKRAGLVSASVSFDGMEGTHDLQRGVKGSWAAALQ
                     AIKNLREADVLVSANTQINKLSLPELEQVLELIIENGVHSWQIQLTVAMGRAADRPEW
                     ILQPYDLLELFPLLERIAARCKEADVLLWPGNNVGYFGPLESTLRGTLPRGHHVGCGA
                     GVWGIGIEADGTIKGCPSMATETWGSGNVRDARLVDIWQRAAPMRFNRDRGVESLWGY
                     CRECYYADVCKAGCTWTSEALLGKPGNNPMCHHRALEMQRAGKRERLVQVQRAPGLPF
                     DQSKFELIVEDL"
     gene            2768..3544
                     /locus_tag="VMZ53_13985"
     CDS             2768..3544
                     /locus_tag="VMZ53_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29617.1"
                     /translation="MRIAFGLVVFLAACGSDSDMLPVGGGSGNGLPDSGMTNNVDAHL
                     IDARMIDGAPLTIDAALFSGRVCLLTDPRDFNSCSATGAANLTVHIGGSVAITAADGT
                     FKIAAPQGGASTWSVTGSNIVSSYKVKNDYFIPALTRTMYDAMKTANGIFLAQGEGSI
                     MVRVIHNGSGYAGSTASASTQPNPAKYLPFYDGSDPTTWNHAATSTTGAQGTIWLVGY
                     DVGDAQFTIAPPAGGGTAVTESGQPVNDQAITWDDVIING"
     gene            complement(3541..5040)
                     /locus_tag="VMZ53_13990"
     CDS             complement(3541..5040)
                     /locus_tag="VMZ53_13990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29618.1"
                     /translation="MSADRYPKAGTVLLGKYRVEAVVGEGGMGAVIKAWHLDLEEPVA
                     LKILLPEMMDRKEIVTRFTREAKAAVKLKGEHVARVLDVGRLEPDADGHFDGTPYIAM
                     EFLEGADLNQIVKAHGPQEASLACDMMLQACEAIAEAHSLGIIHRDIKSSNFFITQAE
                     DQPPHLKVLDFGIATPPKTTSDLTDGESVIGTPAYMAPEQMRSSRQADARSDIWSMGV
                     VLYELLEGRKPFRSEAYSDLCLKVGMDPPDAMVHPDVPEALRAVVMKCLEKTVERRYQ
                     SAAELAFDLLPFASDPVRARASVEQTARLLSRRGTRAFDASRAPDDVTPGAGPKRLTP
                     PAQPAQQSPISESHPSGLTPAPGVPIFDPNAQRTPTSNGQMSPLVTPKRRGVVVAASF
                     ATVIVLAGGGALLFGGKKHPPAAATRPADPQPTEMTETKVEEPATPVQVKETPVEVPA
                     AGSADVAETGSADEGSAANTGSATEKKVIKKRGTGGRVIKKNDDPFGRR"
     gene            complement(5111..6370)
                     /locus_tag="VMZ53_13995"
     CDS             complement(5111..6370)
                     /locus_tag="VMZ53_13995"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HUS29619.1"
                     /translation="MGRLALLVLVCVAGCKVPNAHRDDVDASLSESCGDGVLNGGEIC
                     DDGNTAGGDGCAANCLSDESCGNGVIDDSAGETCDDDNTVAGDGCSADCSSDETCGNG
                     VVDTIKGESCDDANSVSGDGCGKNCQTNEDCGNGTVNPGEECDSGNQFTAQCNPNCTK
                     PACGDGIRNAPAGEECDDHNTSNNDACDAMCRNVVCGNLRVEGPEQCEDGNTKTNDAC
                     INCMNARCGDMYKWPGHEDCDDGNAVQTDSCLNNCTDPTCGDTFIRVGFEDCEDGNTI
                     TTDGCPNCHFARCGDGFKYNGVENCDDGNTTTETACPYGTATCTRCNATCTTSLALTG
                     NVCGDNVRATANEACDDGNTITEAACPYGTPTCTKCSGNCLTSLSLTGPYCGDGMINA
                     GNGEICDDAAMNGMTGFCNASCNGITP"
     gene            complement(6384..7247)
                     /locus_tag="VMZ53_14000"
     CDS             complement(6384..7247)
                     /locus_tag="VMZ53_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29620.1"
                     /translation="MKRLLFLLPLAGCAQLLGLDNTELEQKDARVDAPSVCDGAPRCT
                     STTGRSVCGQLSGTGAMGGMPMRVAAPTGMLCSETGSSEGPCALSIQGQSKASFYAAN
                     GVDVIAGEIDDCGRFVVPDLDATATDVAVVAKGGTDFVDSAALLLGRPAMAGTDMNVQ
                     LPVVSAATETAWGMQLSSTNPPTMTGGYLVSYVDMNGVVVEGLEIRMNTGSGSGPVGK
                     PPSTPWGAYFTGSFDMFDATLTTTQTSGTAAVHPPAGTFMLGGFRTGRTCTQVSLQGV
                     PTAIVHVVVRC"
     gene            complement(7244..8227)
                     /locus_tag="VMZ53_14005"
     CDS             complement(7244..8227)
                     /locus_tag="VMZ53_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29621.1"
                     /translation="MRALLVVMAFLAVPSAAFADKASKARADKLFEEGRGYLQRKEYA
                     LACTAFEQSQHADAAIGTQLNIALCYEEWGKLAAAYKAYLEAERQAKAKKDNREKVAH
                     KKVGELEPKLSRLRVSIPPSADPYSIFLFDGKEVSREDLVEEQLLDAGKHTIEVRIAG
                     SAPKTTPFDLAPGERKSVTLEVPVAIATPPPPPNGGSGPAGGATQTTVVTPRKKNKLY
                     GGIGLIGGGTIAIGIASYVALIARSDYNTAILNCHDQKCPNRADYDATQDARGRANVA
                     TFVTGAGVAMIGVGVYLVLTSKGDPVTVEKVSRVRPMITNDGIGLAIGGSL"
     gene            complement(8251..10275)
                     /locus_tag="VMZ53_14010"
     CDS             complement(8251..10275)
                     /locus_tag="VMZ53_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HUS29622.1"
                     /translation="MPAVSRVLVGIFTLAAACGGASDVVQPSDLPHRSNADVDWRDQV
                     IYQIVIDRFANGDPNNDIHVEPSVPGKFHGGDWQGVIDNLDYLETLGVTALWISPVVK
                     NVEEDAGFHSYHGYWTQDFLRPNAHFGDMSKLRELIDKAHEKNMLVILDVVTNHMGQL
                     FYYDINGNGQPDDGLFGGGYGHTCLQICNQNPTACSTDEMEYCTKGKDYLERIIEWDP
                     DYDPRGVQGWTSLGFSGPADVRFTDWPDKNRTPPPRPPEWFKWPDDKPWFDDPNWYNR
                     RGRVYVWWHEADYTNDFVRDQETKGDFPGGLKDLNTDNPDTKEALIRAFEYWIEVADF
                     DGFRIDTVKHIDRPEVNRNVRGFWGDFADRMRKKAHDLGKQNFFIFGEAFDGKDDLIG
                     SYTWGGQDAQGKFGRLDSVFYFSQKYRGIDAVFAQGQPTKNLECLYNSRVGRAEPMGN
                     YCQMNGYPAGPDYQPTPHAASADGGIGLAPQQVLVNFLDNHDLPRFMFEKNDPAILKV
                     ALAYLMTWDGIPCVYYGTEQGFAGGVDPKNREDMFLGNRALDLAPFATDHELFQYVHG
                     LIQMRKDNIALRRGTVTPVWSTTTTTGPDTGIFAFERSVPGDQTVLVVINASTQTSHT
                     CAGASNCLKTTLSGTLTDVMPGANGATFTVSGGAIDVTMPPRSARVLVKK"
     gene            10446..>11374
                     /locus_tag="VMZ53_14015"
     CDS             10446..>11374
                     /locus_tag="VMZ53_14015"
                     /inference="COORDINATES: protein motif:HMM:NF039689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6209 family protein"
                     /protein_id="HUS29623.1"
                     /translation="MLRLALLAVLVAACVDAPAEEAVNAPQPLVGVDGSHDSADRNCN
                     VVLRDLQRTFTGLSYLTNGSSWVWQGTVEISQAAAAEGLQPYVLYHLAPNGPWQEVAA
                     VPVQAAATPGFVRYTVTLDKDLPGPGWSGTSLSNARIESVPFLKMPQGGRLFDHNRNA
                     GETANYVMTNPDLAIWRDDSRCAPPAGPTRAKVNFNADWTISREGVLAPGGELTITYA
                     QTRLDGCKKTQNGYQLWDITAHVKFEPGGQLIGASVRGGYATMTVPNDARRAVVYFES
                     VNTSGCHQFDSNYGNNYPFDCATPPQWVGNASTL"
BASE COUNT         1864 a         3743 c         3665 g         2102 t
ORIGIN      
        1 acccgatgga atgcgcgtgg ccggtgtcgt ggagaacagc atggcgatgc gcgcggggct
       61 ccttccgggc gatatcgtga cttcgatcgc gaaacagccg acgcgcaatt tgtgcgagct
      121 gtcccatgcg ctgcgcgtcg ccggtcgggc tccgaccacg cagttgattt tcgtgcgggg
      181 cgccgatctc tacgtgcgtg aggtcgaggc catcgagctc gcgcgcgatc cgaacgtgac
      241 ctacggcgag caccgtggtc tgcgcacgct cgcgacgcag ccgccgaacg cgcgcgcgct
      301 gatcgtcttt ctgcagggca tcgcgtgcga gtccgtcgac gcgccgctcg cagacacgcc
      361 actcgaagcg ctcatccaat cgtggaccgc cgccggttat gcgacgctgc ggttcgacaa
      421 gcgcggcatc ggcgatagcg atggtgggcc gtgtcgcgag atcgatttcg cgacagagct
      481 cgcggatgca cgggcgatcg tcgagcacgc ggcgtcacgc ggtctaccac tcatcatctt
      541 cggccactcc gtcggtggca tcatggcgcc gctgctcgcg aagcacgcat ccgcgctcat
      601 cgtctacggc acgccggtca tgccgtggct cgagtgcttg caagacagcg ttcgccggca
      661 gctcgagctt cgccacgtcg gcgagcacac gatcgtcgag caagtggctc agctcgcgcg
      721 gctcgccgac gacggagagc tcaacggtcg ctccgccgcg tatcaccgcc agctcgccga
      781 gctcgatatc gccgcggcgt ggcgcaccgt cgacgctccg gtcctcgtcg tgcgaggcga
      841 gcacgattgg gtcgtgcgtc ccgacgatca agcgcgaatc gccgagctcg ctcgcggcgc
      901 cgtcacgatc gtcgatgttc ccgcgctcga tcacctgttc ggcgcgcacg cggatcgcga
      961 cgcgagcttg cgcgactacg gcatcggtgc cgcgcggggc ggcgattcgc tcgcacgcgt
     1021 gacgctcgac tggctcgcgc gtggtgtagc ctaaccgcta tggagctgtc gccctgtccc
     1081 gcgtgcaagc gccacgttcg gatcgcagcc gcgagctgtc cgttctgcgc gacgcgattg
     1141 ccggtgctgc aggcgcggcg cgcgattccg cgctcgctca cccgcgccgc gatcttttcc
     1201 gcggcgcttg ccggctgcca aacgaaacag tccgcgccag caccgtcggc tggaagtgcc
     1261 gcgccgatcg tcgctccgcc ggcgcccgtt gccgatgccg ccgacatgcc gatcaccgga
     1321 tcgaacgccg gctcggccgt ggttgccgac gacgcgagcg ttgccgcgga cgccgctgcg
     1381 gccggtagtg gtgtcggctc ggctctacgt caggagaagc tgcaccgcct caaggagaag
     1441 cggacgcagc acatcaagat tccggacgaa cgccagatgg cgaagccgta cggtgctccg
     1501 cccgctcgcc gccgcgtcgt ctagagatct tcgacgatca gctcgaactt gctctgatcg
     1561 aacggcaggc caggcgcgcg ctggacctga acgagacgct cgcgcttgcc tgcgcgctgc
     1621 atctcgagtg cgcggtgatg gcacatcgga ttgttgcctg gcttgccgag cagcgcttcg
     1681 ctcgtccacg tgcagcccgc tttgcagacg tcggcgtagt agcactcgcg acagtagccc
     1741 cacagcgatt cgacaccgcg gtcgcgattg aagcgcatcg gtgcagcgcg ctgccagata
     1801 tcgacgagcc gcgcatcgcg cacgttgcca gagccccacg tttcggtcgc catcgacgga
     1861 cagcctttaa tcgtgccgtc ggcttcgatg ccgatgcccc acacgcccgc gccgcaaccg
     1921 acgtgatgac ctcgcggcag cgtgccgcgc aacgtgctct cgagcggacc gaagtaaccg
     1981 acgttgttgc caggccaaag gaggacgtcg gcttctttgc agcgcgccgc gatgcgctcg
     2041 aggagcggga acagctcgag aagatcgtac ggctgcagga tccactcggg acgatcggcg
     2101 gcgcggccca tcgcgaccgt cagctggatc tgccagctgt gcacgccgtt ctcgatgatg
     2161 agctcgagaa cttgttcgag ctcgggcagc gagagcttgt tgatctgcgt gttcgccgag
     2221 acgagcacgt cggcttctcg cagatttttg atcgcctgca gagcggctgc ccacgagccc
     2281 ttcacgccgc gctgcagatc gtgcgtgccc tccatgccgt cgaacgaaac gctcgcgctg
     2341 acgagccccg cacgcttcgc cgcatgtgcg cgctcgagcg tcatgttgcg gccaccggtc
     2401 gtgagcagcg gctccatgcc tcgctcggcg atgcgagcga tgatttcgca ccagtcctcg
     2461 cggaggtacg actcaccgcc gatgagcacc acttcaataa ctccgagctc ggcgagctga
     2521 tcgacgaggc tcaaggcttc agcggtactg agctcgtcgg ggcgtgcctt gccagcgcgc
     2581 gatccgcagt ggccgcacgc gaggtcgcag gcgagcgtga tctcccacac ggcgaggatg
     2641 ggacgagcgg ctacatcgat ctcgcgtacc tgctcggcga ggggcaggtt gcggcgcgcg
     2701 ggcggcgcct tcggtgcgag cggcagcttc ttcacctggg tggtcgtatc acgtaagatc
     2761 gcaagtaatg aggattgcct tcggtctggt cgtgtttctg gcggcgtgcg gcagcgactc
     2821 ggacatgctg ccggtcggtg gcggatcggg caacggcctt cccgatagcg gcatgacgaa
     2881 caacgtcgat gcgcatctca tcgacgctcg catgatcgac ggtgcgcctc tcacgatcga
     2941 cgccgcgctg ttctcgggcc gcgtctgtct gctgaccgat ccgcgcgact tcaactcgtg
     3001 ctcggcgacc ggtgctgcga acctgaccgt gcacatcggc ggcagcgtcg cgatcacggc
     3061 ggccgacggc acgttcaaga tcgctgcacc tcaaggcggc gcgtctacgt ggagcgtgac
     3121 cgggtcgaac atcgtcagct cgtacaaggt caagaacgac tacttcatcc cggcgcttac
     3181 gcgcacgatg tacgacgcga tgaagaccgc gaacggaatc tttctcgcgc agggcgaggg
     3241 ctcgatcatg gtgcgcgtga tccataacgg ctcgggctac gccggctcga cggcctctgc
     3301 gtcgacgcag ccaaatcccg cgaagtacct gccgttctac gatggcagcg atccgacgac
     3361 gtggaaccac gccgccacga gcacgacggg ggcacaaggc acgatctggc tcgtcggtta
     3421 cgacgtcggc gatgcgcagt ttacgatcgc gccccccgcg ggcggaggca ctgcggtcac
     3481 ggagtcggga cagcccgtga acgatcaggc gatcacgtgg gatgacgtga tcatcaacgg
     3541 ctagcgacga ccgaacggat cgtcgttctt cttgattacg cggccgccgg tgccgcgttt
     3601 tttgatcact ttcttctcgg tcgcggagcc tgtgttcgct gccgagcctt cgtcggcgga
     3661 gccggtctct gcgacgtcag cggaaccagc cgccggtact tcgacgggcg tctccttcac
     3721 ctgcaccggc gtcgcgggct cttcgacctt cgtctcggtc atctccgtcg gctgtggatc
     3781 tgcgggccgc gtggcagcag cgggcggatg cttcttgccg ccgaacagca gcgcgccacc
     3841 gcctgcgagc acgatcaccg tcgcaaagct cgctgcgacg acgacaccgc gccgcttggg
     3901 cgtgaccagc ggcgacatct ggccgttgct cgtcggcgtc cgctgcgcat tcggatcgaa
     3961 gatcggcaca cccggtgccg gcgtcaggcc tgaaggatgg gactcggaga tggggctttg
     4021 ctgcgcgggc tgggcgggcg gcgtcagccg cttcggtccg gcgcctggcg tgacgtcgtc
     4081 gggcgcgcgc gaggcatcga acgctcgtgt accgcggcgg ctcagcaggc gcgccgtttg
     4141 ctcgaccgac gcacgcgcac gcacgggatc cgacgcgaac ggaagcagat cgaacgcgag
     4201 ctcggcggcg gactgatagc ggcgctcgac cgtcttctcg agacacttca tcacgaccgc
     4261 gcgcagcgct tcgggaacgt cggggtgaac catcgcgtcg ggcggatcca tgccgacctt
     4321 gagacacaaa tccgagtacg cctcggatcg gaatggcttg cggccctcga ggagctcgta
     4381 gagcacgacg cccatcgacc agatatcgct tcgcgcatcg gcctggcggg acgaccgcat
     4441 ctgctcgggt gccatgtacg ccggcgtgcc gattaccgat tctccgtcgg tgagatcact
     4501 cgtcgtcttc ggcggcgtcg cgattccaaa atcgagaacc ttgagatgcg gcggctgatc
     4561 ctcggcctgc gtgatgaaga agttcgacga tttgatgtcg cgatgaatga tgccgagcga
     4621 gtgcgcttcc gcgatcgctt cgcacgcttg cagcatcatg tcgcacgcga gcgatgcttc
     4681 ttgaggaccg tgcgctttga cgatctgatt caggtccgca ccctcgagga actccatcgc
     4741 aatgtatggc gtgccgtcga agtggccatc ggcgtccggc tcgagcctgc cgacgtcgag
     4801 cacgcgcgcg acatgctcgc ccttgagctt caccgcggct ttcgcttcgc gcgtaaagcg
     4861 cgtcacgatc tccttgcggt ccatcatctc gggcaggagg atcttcagcg cgaccggttc
     4921 ctcgaggtcg aggtgccacg ccttgatgac ggctcccatg ccgccttcgc cgacgacggc
     4981 ttcgactcgg tacttgccga gcaagaccgt gcctgccttg ggataccgat cggccgacaa
     5041 cgcgttctca gtctaaccga tcagtaaccg gcacgacaaa gtgcgcggca cggtaggtct
     5101 gctgcctaca ctacggcgtg atgccgttgc agctcgcgtt gcagaaacca gtcattccat
     5161 tcatcgcggc gtcgtcgcaa atctcgccat tgccggcgtt gatcatccca tcgccgcagt
     5221 agggcccggt cagcgacagc gacgtcaggc agttcccgct gcatttcgta cacgtcggtg
     5281 tcccgtacgg acacgcggcc tcggtgatgg tgttgccgtc gtcgcacgcc tcgttcgcag
     5341 tcgcgcgcac gttatcgcca cacacgttgc cggtgagcgc gagcgacgtc gtgcaggtcg
     5401 cattgcatct cgtgcaggtc gcggtgccgt agggacatgc ggtttccgtg gtcgtgttgc
     5461 cgtcgtcgca attttcgacg ccgttgtact tgaagccgtc gccacacctc gcgaagtggc
     5521 agttcggaca tccgtccgtc gtgatcgtgt tgccgtcctc gcaatcctcg aagcccaccc
     5581 ggatgaacgt gtcaccgcac gtcgggtccg tgcagttgtt caggcacgaa tcggtttgca
     5641 cggcgttgcc gtcatcgcag tcctcgtggc cgggccactt gtacatgtcg ccgcagcgcg
     5701 cgttcatgca gttgatgcag gcgtcgttcg tctttgtatt gccgtcctcg cactgctcgg
     5761 gaccttcaac gcgaaggttg ccgcatacga cgttgcgaca catcgcatcg cacgcgtcgt
     5821 tgttgctcgt gttgtgatcg tcgcattcct cgccggcggg tgcgttgcgg atgccgtcgc
     5881 cacacgcggg cttcgtgcag ttcggattgc actgcgcggt gaactgattg cccgagtcgc
     5941 attcctcgcc gggattcacg gtgccgttac cgcagtcttc gttggtctgg cagttctttc
     6001 cgcaaccatc tccggacacg ctgttcgcgt cgtcgcagct ctcgcctttg atcgtgtcga
     6061 cgacgccgtt gccgcacgtt tcgtcggacg aacagtcggc gctgcagcca tcaccggcaa
     6121 ctgtgttgtc gtcgtcgcac gtctctccgg cggagtcgtc gatcacgccg ttgccgcagg
     6181 attcgtcgga caagcaattc gccgcacagc catcaccgcc ggcggtgttg ccgtcgtcgc
     6241 agatctcgcc gccgttcaag acgccatcgc cgcacgactc ggacaggctc gcgtcgacat
     6301 catcgcgatg cgcgttcggc actttgcagc cggcgacgca tacgagcacg agcagcgcga
     6361 gacgacccat catcgtcatc ctatcaacac cgcacgacga cgtggacaat cgccgttgga
     6421 acgccctgta gggatacctg cgtacacgtc cggcccgtgc gaaagccacc gagcatgaac
     6481 gtaccggcgg gaggatggac cgcagcggtt ccgctggtct gggtcgtcgt gagagttgcg
     6541 tcgaacatgt cgaacgatcc cgtgaagtac gcaccccacg gcgtcgacgg cggcttgccg
     6601 acggggccgc tgccggagcc ggtgttcatg cgaatctcga gaccctcgac gacgacacca
     6661 ttcatgtcga cgtacgacac gaggtagcca ccagtcatcg tcggcggatt ggtcgagctc
     6721 agctgcatgc cccacgcggt ctcggtcgca gcgctcacga ccggtagctg cacgttcata
     6781 tcggtgcccg ccatcgcagg cctaccgagc aggagcgcgg ccgagtcgac gaagtccgtc
     6841 ccgcctttgg caacgaccgc aacatcggta gccgtggcgt cgagatcagg cacgacgaag
     6901 cggccgcagt catcgatctc gccggcaatc acgtcgacgc cgttcgcagc gtagaagctc
     6961 gccttcgact ggccttggat cgagagcgcg cacggtcctt cactcgagcc ggtctcggag
     7021 cacagcatgc ccgtgggtgc agcgacgcgc atcggcatgc cacccatcgc gccggtgccg
     7081 ctgagctgcc cgcacaccga gcggccggtc gtcgacgtgc agcgaggcgc accgtcgcac
     7141 accgaaggcg catcgacgcg tgcgtccttc tgttcgagct ccgtgttatc gaggccgagg
     7201 agctgcgcgc agccggccag tggaaggagg aacagcagcc gcttcacagc gagccgccga
     7261 tcgcgagccc gatgccatcg ttcgtgatca tcggccgcac gcgagacacc ttctcgacgg
     7321 tgacagggtc gcctttgctc gtcagcacga ggtacacgcc gacgccgatc atcgcgacgc
     7381 ccgcgccggt cacgaaggtc gcgacgttcg ctcggccgcg cgcgtcttgg gtcgcgtcgt
     7441 aatcggcgcg attcggacac ttttgatcgt ggcaattcaa gatcgcggtg ttgtagtcgg
     7501 agcgagcgat gagcgcgacg tagctcgcga tgccgatcgc tatcgtcccg ccgccgatca
     7561 ggccgattcc gccgtagagc ttgttcttct tgcgtggcgt cacgacggtc gtctgcgttg
     7621 cgccgccggc tgggccggag cctccattcg gcggtggtgg cggtgtcgcg atcgcgacgg
     7681 ggacctcgag cgtcaccgac tttcgctcgc ccggagcgag atcgaatggc gtggtcttcg
     7741 gcgcgctgcc ggcgatgcgg acttcgatcg tgtgcttgcc cgcatcgagc agctgctcct
     7801 cgacgagatc ctcgcgactc acttctttcc cgtcgaacaa gaagatcgaa tacggatccg
     7861 cgctcggcgg gatcgacacg cgcaggcgcg acagcttcgg ctcgagctcg ccgaccttct
     7921 tgtgcgcgac cttctcgcga ttatccttct tcgccttcgc ctggcgctcc gcctcgaggt
     7981 acgccttgta cgccgcagcg agcttgcccc actcctcgta gcagagcgcg atattcagct
     8041 gcgtgccgat cgctgcatct gcatgctggc tctgctcgaa cgcggtgcac gcgagtgcgt
     8101 actccttgcg ctgcaggtag ccgcgacctt cctcgaacag cttatccgcg cgcgccttgc
     8161 tcgccttgtc agcgaacgcg gcggacggca cggccaagaa ggccatgacg accaggaggg
     8221 ctctcatcgc cacgatcgta tcgcgaaagc ctacttcttg acgagaacgc gtgccgatcg
     8281 cgggggcatc gtgacgtcga tcgcgccgcc gctaaccgtg aacgtggcgc cgtttgcacc
     8341 gggcatcacg tcggtgagcg tgcccgagag cgtcgtcttc aagcagttcg atgcgcccgc
     8401 gcacgtgtgg ctcgtctgcg tgctcgcatt gatgacgacg agcaccgttt ggtcgccagg
     8461 aacgctgcgc tcgaacgcga agatgcccgt atcgggtccg gtcgtcgtcg tcgtcgacca
     8521 caccggcgtc acggtaccgc ggcgcagcgc gatgttgtcc ttgcgcatct ggatcaggcc
     8581 gtggacgtat tggaacagct cgtggtccgt cgcgaacggc gcgagatcga gagcgcgatt
     8641 gccgaggaac atgtcctcgc ggttcttcgg atcgacgccg ccggcgaagc cctgctcggt
     8701 gccgtagtag acgcacggga tgccgtccca cgtcatcagg taggcgaggg cgaccttcag
     8761 gatcgccgga tcgttcttct cgaacatgaa gcgcggcaga tcgtggttgt cgaggaagtt
     8821 gacgagcacc tgctgcggcg cgaggccgat gccaccgtca gcagacgcag cgtgcggcgt
     8881 cggttggtaa tcggggcccg ccgggtagcc attcatctgg cagtagttgc ccatcggctc
     8941 ggcgcgaccg acgcgcgagt tgtagaggca ttcgaggttc ttcgtcggct gaccctgtgc
     9001 gaacacggcg tcgatgccgc gatacttctg cgagaagtag aagaccgagt caaggcgccc
     9061 gaacttgccc tgcgcgtctt ggccgcccca cgtgtagctg ccgatcagat cgtctttgcc
     9121 gtcgaacgct tcaccaaaga tgaagaagtt ctgcttgccg agatcgtgcg ccttctttcg
     9181 catgcgatcg gcgaagtcgc cccagaagcc gcgcacgttg cggttcacct cgggacgatc
     9241 gatgtgcttg acggtgtcga tgcggaagcc gtcgaagtcc gcgacctcga tccagtactc
     9301 gaacgcgcgg atgagcgctt ctttcgtgtc gggattatcg gtgttgagat ccttcaggcc
     9361 gcccgggaag tcgcccttcg tctcctgatc gcggacgaag tcgttcgtgt agtcagcctc
     9421 gtgccaccag acgtagacgc gaccgcggcg gttgtaccaa ttgggatcgt cgaaccacgg
     9481 tttgtcgtcg ggccacttga accactcggg cggacgcggc ggcggcgtgc ggttcttgtc
     9541 aggccagtcc gtaaaccgga cgtcagcagg accggagaac ccaagtgagg tccagccctg
     9601 cacgccgcgc gggtcgtagt ccggatccca ctcgatgatc cgctcgaggt agtccttgcc
     9661 cttcgtgcag tactccatct cgtcggtgct gcacgcggtc gggttctgat tgcagatctg
     9721 caggcacgtg tggccgtagc cgccgccgaa caagccgtcg tcgggctgac cgttgccatt
     9781 gatgtcgtaa tagaagagct gccccatgtg gttcgtcacc acatcgagga tcacgagcat
     9841 gttcttctcg tgtgctttgt cgatcagctc gcgcagcttc gacatgtcgc cgaagtgagc
     9901 gttcggccgc aagaagtcct gcgtccagta gccgtgatag ctgtgaaagc cggcatcctc
     9961 ctcgacgttc ttcacgaccg gagaaatcca cagtgcggtc acaccgagcg tctcgaggta
    10021 atcgaggttg tcgatgacgc cctgccaatc gccgccgtgg aacttgcccg gcacgctcgg
    10081 ctcgacgtgg atgtcgttgt tcggatcgcc gttcgcgaac cgatcgatca cgatttgata
    10141 gatgacttgg tcgcgccagt cgacatcggc gttgctgcgg tgaggcaggt ccgatggctg
    10201 aacgacatcc gacgcgccac cgcatgctgc agcgagggtg aaaattccga cgaggacgcg
    10261 cgacacggcg ggcatgggca gcacgattcg tgccccaaaa tgcgcctcgt gatcaacatg
    10321 ttaaggatca cagtgctcct cggcgcgttg cgaatcgcaa cgagctgcaa acggccgtcg
    10381 cgactaacct cgcgcagccg ctgagggcac cgctggcata ggacgcgcag cccgcgcgaa
    10441 cgcatatgct tcgcctggct ctactggctg ttctagttgc cgcttgcgtc gacgcgccgg
    10501 cggaagaagc ggtgaacgcg ccgcagcctc tcgtcggcgt cgacggcagt catgacagcg
    10561 ccgatcggaa ctgcaacgtc gtgctccgcg acctgcagcg cacgttcacg ggcctcagct
    10621 atctgacgaa cggatcgagc tgggtgtggc aaggcaccgt cgagatttcg caggccgccg
    10681 ccgccgaggg ccttcagccg tatgtgctct atcacctcgc gccgaacggt ccgtggcaag
    10741 aagtcgcggc ggttcctgtg caggccgcgg cgacgccggg cttcgttcgc tacaccgtaa
    10801 ctctcgacaa agatctgccg ggtcccggct ggtcgggcac gtcgctgtcc aacgcgcgca
    10861 tcgagagcgt gccgtttctg aagatgccgc aaggcggccg cctcttcgat cacaaccgca
    10921 acgcgggcga gaccgccaac tacgtgatga cgaatcccga tctcgcgatc tggcgtgacg
    10981 actcgcgctg cgctccgccg gccggtccaa cgcgcgcgaa ggtcaacttc aacgcggact
    11041 ggacaatctc gcgcgaaggc gtgctcgctc ctggcggcga gctgacgatc acgtacgcgc
    11101 agacgcggct cgacggctgc aagaagacac agaacggcta ccagctctgg gacatcaccg
    11161 cgcacgtgaa gttcgagccg ggtggtcagc tgatcggcgc gtcggtgcgc ggcggctacg
    11221 cgacgatgac cgtccccaac gatgcgcggc gcgcggtcgt gtacttcgag tcggtgaaca
    11281 cgagcggctg ccatcagttc gactccaatt acggcaacaa ctacccattc gactgcgcga
    11341 cgccaccgca atgggtcggc aacgcgagca cgct
//
LOCUS       DATLBZ010000267         5887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6856406, whole genome shotgun sequence.
ACCESSION   DATLBZ010000267 DATLBZ010000000
VERSION     DATLBZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6856406"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..1570)
                     /locus_tag="VMZ53_14020"
     CDS             complement(332..1570)
                     /locus_tag="VMZ53_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS29624.1"
                     /translation="MRRILDAFVVALESLAEHKLRTTLTMLGMMFGVGAVIAMLSIGA
                     GAERQALALIDRLGTRNVVVRAKNFKPEELAEIRKKSMGLSQRDVEAIQEAVPGVSFA
                     APKNEIEPYKILAAGAKTKAQVYGVGHRHREVTTFALAEGRFIDLGDEKHHSQVAVIG
                     AGVRRDLFGADVAIGKDIKINDVWFEVVGVLAPEPGAVAAVEGVPVSSTEREIYLPFT
                     TALRKLDREPMKSPLSEIVVRLDANAPAAETGAVIGTLLDRLHGGTRDYEIIVPEALL
                     RHSQQTQQLFNLVMGLIAGISLLVGGIGIMNIMLASVLEQTREIGVRRAVGARRSDIR
                     FQFLVTAFSLALIGGLLGVAMGLGIARGVASYASWPTVVTTWSIALSLGVSVVVGVAS
                     GLYPAVRAAKLDPIEALHAE"
     gene            complement(1567..2862)
                     /locus_tag="VMZ53_14025"
     CDS             complement(1567..2862)
                     /locus_tag="VMZ53_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HUS29625.1"
                     /translation="MKKLAITLVVLAGLGGGGWFVLGKTTDKKDAVPTQKVAKTPFVR
                     RVTAEGNLRAVKATPLAAPMQNGGWDGPVKIAWLAQDGSKVKAGDVVVRFDPSDPEKQ
                     LREAQADLASADAKLAGEAIKSKNAIEGRDQDAESAADELEQQRKFKAKDEQIFSHNQ
                     IVEGQIDEKLAGAKQDHANKAKEIERKLAKSNAGVIAVEKQKAQITISHAKSALESME
                     IKAPHDGILVLRRNWRGQLPRVGEQLWPGQKVAEIPLLDEMEAEVFVLEVDGSGLAND
                     QKAELVIEAQPDVVYNAKIKLVDKLAKPRDPGVPVQYFAVVLTLDKTDKALMKPGQRV
                     RATLILDQEDAIVVPRQAVVMKDGKNVVFKKTKTGTFDPIPVELGPGTSGRVVIAKGL
                     EPTDEIALRDPTLSLDSALGSGSAASTPGGASGGKGGKP"
     gene            complement(2859..4043)
                     /locus_tag="VMZ53_14030"
     CDS             complement(2859..4043)
                     /locus_tag="VMZ53_14030"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HUS29626.1"
                     /translation="MKRLLVLAVIGLGAACSKGSTPAQLVDVKRADLVLGVEVEGELE
                     AVDSTDVKPPALPGVWNYKVSNLGNEGADVKPGELTVAFDSSEQMRELEERNNEAEAA
                     KKKLDKKRDDAALARRNEELEIAQSEANLRKSSLKTTSSPDLVASVDQKVIELDEQLA
                     TLSLEQTKAKAAQAAASDKAEIGQLADHHKFLMDRVAELQANIAKLEVKAPRAGTLVF
                     PTNWRGEKKKVGDPAWRGETVVQVVALGKMRGQGMIDEIDIARVHLGQTVSMHLDALP
                     DVTLRGTIEEIAKSVAPHSGTDPSNIVKVKIAIDPAQKVPLRPGMRFRGEVETERLAN
                     VTQVPAEAVFVTPEGPVAYRADGSRVKLKVGKRNASAIEILDGVKPGDRISRVDPTRG
                     EP"
     gene            complement(4040..5239)
                     /locus_tag="VMZ53_14035"
     CDS             complement(4040..5239)
                     /locus_tag="VMZ53_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29627.1"
                     /translation="MKRFVIYSLGLALCACGGGGGKHAAATEEAATTVKVHKGEVVDR
                     VVLTGSIEATHAEEMRVPRTDAWELPIRWMAEDGAQVKQGDRVLEFDNAQFTAQLQQQ
                     KLAVLDAEINFETFLGVSALQIQDKQFALDSAKIQLEKAKVLAAVPEDILPARTYQER
                     QLDLKQKQRAVEKAEKDLAAAKASASLDRQVKQIELEKARAQIEHAEKSIDALTIKAP
                     RDGIAVVDEHPWEGRKYQIGDTVQPGWTIVSLPEFAAGMEVHAALSDVDDGRINLGMT
                     GTCTLDAYPADPLPCVVKELMPVASGDRESLRRGFAVVISIDKFDRDRMIPGMAVKVE
                     LRRQPLAAVVVPRGAVRFDEAGKKARIRLASGETKDVELDACDAQSCAVKSGVNDGDA
                     VVVGGGR"
     gene            complement(5236..>5887)
                     /locus_tag="VMZ53_14040"
     CDS             complement(5236..>5887)
                     /locus_tag="VMZ53_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS29628.1"
                     /translation="FTVHEGEMVAIVGTSGSGKSTLLNILGMLDTPSGGRYRLGGRDV
                     QDLSDDDRARARNQQIGFVFQSFQLLHRASALKNVELPLQYRGDVPARDRRERATKAL
                     TRVGLAQRMKHKPFQLSGGQRQRTAIARALVTEPSLLLCDEPTGNLDSSTTEDIMALF
                     HRLHEEKHTILIVTHEPAIAARCPRAIRIKDGKIKADGPGAEVAREASTVALGGAA"
BASE COUNT          837 a         1855 c         2018 g         1177 t
ORIGIN      
        1 gcttctgctt ctgcttctgc ttctgcttct gcttctgctt ctgcttctgc gtggcagaga
       61 gcgccgggcc ggcggggagc ggcgcgcccc ggccttcaaa tgggccaggg cgcgcgccgc
      121 gacttcgccg ccggcccgag cgcggctcgc gaaatgttcg tgctccacac gaggtcgaag
      181 cgtgatcgta gtgttcgtcg tgtcgaatgg cagagagcgc cgccggccgg ggcgcggctc
      241 gcgagacttt tctcggtcac acgaacgtgg tcgcgatcga ccgctctcgc gccttcgtac
      301 ttggcgcgga gcgacggggc gcgccgccgg cttactcggc gtgcagcgct tcgatcggat
      361 cgagcttcgc cgcgcgaacg gcggggtaga ggcccgaggc cacgccgacc acgacggaga
      421 cgccgaggga gagcgcgatc gaccacgtcg tgacgacggt cggccagctc gcgtagctcg
      481 cgacgcctcg tgcgatgccg aggcccatcg cgacgcccag gaggccgccg atgagggcga
      541 gggagaatgc ggtgacgagg aactgaaagc ggatgtcact gcggcgggca ccgaccgcgc
      601 ggcgcacgcc gatctcacgc gtttgctcga ggacggaggc gagcatgatg ttcatgatgc
      661 cgatgccgcc gacgaggagg gagatgccgg cgatgaggcc catgaccagg ttgaagagct
      721 gctgggtttg ttgcgagtgg cggaggagtg cttcggggac gatgatctcg tagtcgcgcg
      781 tgccgccgtg gaggcgatcg aggagcgtgc cgatgacggc gcccgtttcg gcggcggggg
      841 cgttggcgtc gaggcggacg acgatttcgg agagcgggga tttcatcggc tcgcgatcga
      901 gcttgcggag ggcggtcgtg aacgggaggt agatttcgcg ctcggtggag gagaccggga
      961 cgccttcgac tgcggcgact gcgccgggct cgggggctag gacaccgacg acttcgaacc
     1021 agacgtcgtt gatcttgatg tctttgccga tggcgacgtc ggcgccgaag aggtcgcggc
     1081 ggacgccggc gccgatgacc gcgacttggc tgtggtgttt ttcgtcgccg agatcgatga
     1141 agcggccttc cgcgagcgcg aatgtcgtga cttcgcggtg gcgatggccg acgccgtaga
     1201 cttgggcttt ggttttggcg ccggcggcta ggatcttgta gggctcgatc tcgttcttgg
     1261 gcgcggcgaa ggagacgccg gggacggctt cctggattgc ttcgacgtcg cgctgcgaga
     1321 ggcccatcga cttcttgcgg atttcggcga gctcttcggg cttgaagttt ttggcgcgga
     1381 cgacgacgtt gcgcgtgccg aggcgatcga tcagggcgag agcttggcgt tctgcgcctg
     1441 caccgatcga gagcatcgca atgacggcgc cgacgccgaa catcatgccg agcatcgtga
     1501 gcgtggtgcg tagcttgtgc tcggctaggc tttcgagcgc gacgacgaat gcgtcgagga
     1561 tgcgcctcac ggcttgcctc ctttgccgcc agaggcgccg cccggggtgc ttgcggcgga
     1621 gccgcttccg agcgccgagt cgagcgagag ggtcgggtca cgcagggcga tctcgtcggt
     1681 gggctcgagg cctttggcga tcacgacgcg gcctgaggtg ccggggccga gctcgacggg
     1741 aattgggtcg aacgttcccg tcttcgtctt cttgaagacg acgttcttgc cgtccttcat
     1801 cacgacggct tggcgcggga cgacgatcgc gtcttcttga tcgaggatga gcgtcgcgcg
     1861 gacgcgctgg ccgggcttca tcagcgcctt gtccgtcttg tcgagcgtga gcacgacggc
     1921 gaagtattgg acgggcacgc ccgggtcgcg cggcttcgcg agcttgtcga cgagcttgat
     1981 cttcgcgttg tagacgacat cgggctgcgc ctcgatgacg agctcggctt tctgatcgtt
     2041 tgcgaggccc gagccgtcga cttcgaggac gaagacttcc gcttccatct cgtcgaggag
     2101 cgggatctcg gcgacctttt ggccgggcca gagctgctcg ccgacgcgcg ggagctggcc
     2161 gcgccagttg cggcggagca cgaggatgcc gtcgtgcggc gctttgatct ccatcgactc
     2221 gagcgcgctc ttcgcgtgcg agatcgtgat ttgggctttc tgtttctcga cggcgatcac
     2281 gccggcgttg gatttggcga gcttgcgctc gatctctttg gccttgttcg cgtgatcttg
     2341 ttttgcgccc gcgagcttct cgtcgatttg gccttcgacg atctggttgt ggctgaagat
     2401 ctgctcgtcc tttgccttga acttgcgctg ctgctcgagc tcgtcggccg cgctctcggc
     2461 gtcttggtcg cgaccctcga tcgcgttctt cgacttgatc gcttcgcctg cgagctttgc
     2521 atcggcggag gcgagatcgg cctgcgcttc acgcagctgc ttctcgggat ccgagggatc
     2581 gaagcgcacg acgacgtcac cggctttcac cttcgagccg tcttgggcga gccacgcgat
     2641 cttgaccggg ccatcccagc caccgttttg catcggtgcc gcgagcggcg tggccttgac
     2701 tgcgcgcaga ttgccttcgg ccgtgacgcg tcgaacgaac ggcgtcttcg cgaccttctg
     2761 cgtcgggacc gcgtccttct tgtccgtcgt cttgccgagg acgaaccagc cgccaccgcc
     2821 gagtccggcg agcacgacca atgtgatggc gagcttcttc atggctcacc tcgcgtcgga
     2881 tcgacgcgtg agatgcgatc gcctggcttc acgccgtcga ggatctcgat cgccgacgcg
     2941 ttgcgcttgc cgactttcag cttcacgcgc gagccatcgg cgcgatacgc gacggggcct
     3001 tcgggcgtca cgaacacggc ctcggcagga acctgggtga cgttcgcgag gcgttccgtc
     3061 tcgacctcgc cgcggaagcg catgcccgga cggagcggca ccttctgcgc gggatcgatc
     3121 gcgatcttca ccttgacgat gttgctcggg tccgtgcccg agtgcggcgc gaccgacttc
     3181 gcgatctctt cgatcgtgcc gcgcagcgtg acgtcgggca gcgcgtcgag gtgcatcgag
     3241 acggtctggc cgaggtggac gcgagcgata tcgatctcgt cgatcatgcc ttggccgcgc
     3301 atcttgccga gcgcgacgac ctgcacgact gtctcgccgc gccacgcggg gtcgccgacc
     3361 ttcttcttct cgccgcgcca gttcgtcggg aacaccagtg tgccggcgcg cggcgctttg
     3421 acttcgagct tcgcgatgtt cgcctgcagc tcggcgacgc ggtccatgag gaacttgtgg
     3481 tgatcggcga gctggccgat ttctgctttg tccgatgcgg cggcctgcgc ggccttcgct
     3541 ttcgtctgtt cgagcgacag cgtcgcgagc tgctcgtcga gctcgatgac tttttgatcg
     3601 accgatgcga cgagatccgg cgagctcgtc gtcttcagcg acgacttcct gagatttgct
     3661 tccgactgcg cgatctcgag ctcttcgttg cggcgagcga gcgctgcgtc gtcacgcttc
     3721 ttgtcgagct tcttcttcgc ggcttcggcc tcgttgttgc gctcttcgag ctcgcgcatc
     3781 tgctcggagc tgtcgaacgc gacggtcagc tcgcccggct tcacgtccgc gccctcgttg
     3841 ccgaggttcg agactttgta attccagacg ccgggcaggg caggcggctt cacgtccgtc
     3901 gagtcgacgg cttcgagctc accctcgact tcgaccccga gcacgagatc ggcgcgcttg
     3961 acgtcgacga gctgtgcagg cgtcgagcct ttactgcagg cagcgccgag accgatgaca
     4021 gcgagcacga ggagccgctt catcggccac ctccgacgac gactgcgtca ccatcattga
     4081 caccgctctt cacggcgcag ctttgggcgt cgcacgcgtc gagctcgaca tctttggtct
     4141 ctccgctcgc gaggcgaatg cgcgctttct tacccgcttc atcgaagcga accgcgccgc
     4201 gcggcacgac gacggctgcg agaggctggc gccgaagctc gaccttcacg gccatacccg
     4261 gaatcatccg gtcgcgatcg aacttgtcga tcgagatcac gacggcgaag ccacgccgca
     4321 gcgactcgcg atcgccgctc gcgacgggca tcagctcctt gacgacgcac ggcaacggat
     4381 cggcgggata cgcatcgagc gtgcacgtgc ccgtcatgcc gaggttgatg cggccatcgt
     4441 cgacatcgga gagcgcggcg tggacttcca tgccggcggc gaactcgggc agcgagacga
     4501 tcgtccagcc aggctgcacg gtgtcgccga tctgatactt gcggccttcc cacgggtgtt
     4561 cgtcgacgac cgcgatgcca tcacgcggcg ctttgatcgt cagcgcgtcg atcgatttct
     4621 cggcgtgctc gatctgcgcg cgcgctttct cgagctcgat ctgcttcacc tggcgatcga
     4681 gcgatgcgga tgccttcgcc gctgcgagat ccttctcggc tttctcgacg gcgcgctgct
     4741 tctgcttgag atcgagctgg cgttcttgat acgtgcgcgc cggcaagatg tcctcgggaa
     4801 cggccgcgag gaccttggct ttttcgagct ggatcttggc gctgtcgagc gcgaattgct
     4861 tgtcttggat ctgcagcgcg ctgacgccga ggaacgtctc gaagttgatc tccgcgtcga
     4921 gcacagcgag cttctgctgc tggagctgcg cggtgaactg cgcgttgtcg aactcgagca
     4981 cgcggtcgcc ttgcttgacc tgcgcgccgt cttccgccat ccagcgaatc ggcagctccc
     5041 acgcatctgt gcgtggcacg cgcatctctt cggcgtgcgt tgcttcgatg ctgccggtca
     5101 gaacgacgcg atcgacgacc tcgcccttgt gcaccttgac cgtggtcgcg gcttcttccg
     5161 tcgccgcagc gtgcttgccg ccgccgccac cgcatgcgca gagcgcgagg ccgagagagt
     5221 agatgacaaa gcgtttcacg cggcgcctcc caaagcgacc gtcgaggctt ctcgcgcgac
     5281 ctcggcgcca gggccatccg ctttgatctt gccgtccttg atgcgaatgg cgcgcgggca
     5341 ccgagcggcg atcgcaggct cgtgcgtgac gatgaggatc gtgtgcttct cttcgtgcag
     5401 gcgatggaac aacgccatga tgtcctcggt cgtcgaggaa tcgaggttgc ccgtcggctc
     5461 gtcgcacagc agtagcgaag gctccgtcac gagcgcgcgc gcgatcgcag tgcgctggcg
     5521 ctgaccgccc gagagctgaa acggcttgtg cttcatgcgc tgggcgaggc cgacgcgcgt
     5581 cagtgccttc gttgcacgct cgcggcgatc gcgtgccgga acgtcaccgc ggtattgcag
     5641 cggtagctcg acgttcttca gcgcgctcgc gcgatgcagc agctggaagc tctggaacac
     5701 gaagccgatc tgctgattgc gagcgcgcgc gcgatcgtcg tcggagaggt cctggacgtc
     5761 gcggccacca aggcggtagc ggccgccgga tggtgtgtcg agcatgccga ggatgttgag
     5821 cagcgtcgac ttgccggagc ccgaggtccc cacgatggcg accatctctc cctcatgcac
     5881 ggtgaag
//
LOCUS       DATLBZ010000268        17238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6109692, whole genome shotgun sequence.
ACCESSION   DATLBZ010000268 DATLBZ010000000
VERSION     DATLBZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17238
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6109692"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VMZ53_14045"
     CDS             <1..908
                     /locus_tag="VMZ53_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29629.1"
                     /translation="ENTGSGSANVVQNNGSAGSAVGVVATGSDTGSAGTMTGSDNGSA
                     TTMVGSGAGKPVDTVIASAAKGAMVEIEGTDMKGPAPFTAKLEKDRAYKARVTAPGFV
                     AQEIEVKGGAPKVTAKLAAKSKIISVTTDPPGADILVDGVPTSKLTPSDVQLTAAQSA
                     RARVRITLRRPGYKQQDQYVETAAWNEGDSSMTASVTAKLVVAPKVVPNNNGNTTTNT
                     GSGSGSAGTGDGSATTPTGGGTGSASGTTTTPPTGGTTTPPTGGTTTPPAGGTTTPPS
                     GGTTPPSGGTTPPATGSASGGQTLK"
     gene            915..1412
                     /locus_tag="VMZ53_14050"
     CDS             915..1412
                     /locus_tag="VMZ53_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29630.1"
                     /translation="MRVVLLALLLAAACKEKDDKKSAKPVVVDPRPLARGSSNGSATP
                     TPVAVEAHKAPAAPMQIEDARAVLPKIEGNVILDLKQTSDKRQVHATWCIDGTGADEV
                     AKLVGKWMSDAGYTALSIRGDARKAGVSGDRGEIRLSMVVASSGAANCPAPTHYFASA
                     TIFRP"
     gene            1533..2129
                     /gene="raiA"
                     /locus_tag="VMZ53_14055"
     CDS             1533..2129
                     /gene="raiA"
                     /locus_tag="VMZ53_14055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HUS29631.1"
                     /translation="MQFAVTFRHMEPTDALKSYARERMERVRKYLPDPIACHVVLSTE
                     RHNHRIDVNFQLHNGLSIAGHETTENMYSSIDLCIAKIERQVRKYKGKLEGMRARPHH
                     VVALPWSHSIIDEAFHELKDDGANGHAATKPAPEFTVVKTEKFHAQPMSVSDAIVQLN
                     LLHEPFLVFRADTDGRVAVVYKREDGSYGLIETPTASS"
     gene            2164..2625
                     /locus_tag="VMZ53_14060"
     CDS             2164..2625
                     /locus_tag="VMZ53_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HUS29632.1"
                     /translation="MKIVDLIKRDMVVPALQATDKRGILEELAAYMSAHHTRIDRASL
                     SKVLIEREQLASTAIGEGVAIPHGKLGAVGEIVACLGRAPTGVEFDSMDGQPTFLFFV
                     LVAPESSTGAHLKALARISRVFKDPEFRRRLMAAGDAESMYHVVADEDAKY"
     gene            2626..3609
                     /gene="hprK"
                     /locus_tag="VMZ53_14065"
     CDS             2626..3609
                     /gene="hprK"
                     /locus_tag="VMZ53_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HUS29633.1"
                     /translation="MSGSITVGELLERAELGVTTVAGASGLGRTVIVPRIQKPGLALT
                     GWKEQLHSNRVLVLGGTEIEYLADHEEARKNGIATMLASDPACVVICRGLDAPDELVH
                     ACEERGVPLVQSTLVTADFIHAVTAWMADRLAPSTERHGVLMDVLGIGVLMTGKSGIG
                     KSETALDLVMRGHRLVGDDVIRIRRQGSALSGRGAGILGHHMEIRGLGIINIKDLFGI
                     SAVREAKVIDLIVELHEWDADAEYDRLGFDDQTERLLDYPVPKLRLPVRPGRNLATLI
                     EVAARNQLLKQQGIHSAKAFRDKLHRAMGSAGSPSELDSLHHRNDGPGATE"
     gene            3610..4446
                     /gene="rapZ"
                     /locus_tag="VMZ53_14070"
     CDS             3610..4446
                     /gene="rapZ"
                     /locus_tag="VMZ53_14070"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HUS29634.1"
                     /translation="MQIVIVTGLSGAGKSTALRALEDIEFTTVDNIPVPLVGQLVEQL
                     AHEGDRDKVAIAIDARQRRNLGMWAQMLALLRGAGHRLEVMFLDANDEVLLRRFSETR
                     RRHPLSGDDLIEGVRRDREVMTEMRHGAAVVDTSSFNMHQLKKIIQERYGGTGRLAVT
                     IVSFGFKHGLPLEFNLVFDVRFLPNPFFDPALTHLTGRDPEVVKFVMGPEGTQYLEHI
                     ESMLRFALPQFQKEGKLYVTVCVGCTGGRHRSVAVVEELRRRLGQEWDILIRHRDVER
                     GE"
     gene            4446..4724
                     /locus_tag="VMZ53_14075"
     CDS             4446..4724
                     /locus_tag="VMZ53_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HUS29635.1"
                     /translation="MAAVAEKTLKIQNELGLHARAATKLVQLASKYPCEITVTKDGNE
                     VNGKSIMGVLMLVASKGTTVTVRAKGDKADEAVAAIAALIDDKFGEGK"
     gene            4729..6501
                     /gene="ptsP"
                     /locus_tag="VMZ53_14080"
     CDS             4729..6501
                     /gene="ptsP"
                     /locus_tag="VMZ53_14080"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HUS29636.1"
                     /translation="MTAAREEVRKTGLGVSAGIAFGRAYLIGRDTLKAPRHHIEADDV
                     DTEVARLYKAIAASDKQLAKIKEKLASENESDYHIITAHQMMLHDEHLVGAAVEYMRE
                     EMVNAEWALRRAVDDIRSVFDSIEDPYLRERRSDVEFVFERVLRNLLGRDMGPLSPPP
                     DAIVVAYDLSPADTATLHKAAVAGLLTDAGGKTSHTAIIARAHEIPAVVGLENITELV
                     ETDDLLLLDGGTGIVIVNPSAATVAQYRDEQRRQVAEGVRFHAMRDLPARTRDDLDVA
                     LLANIDGPDELDDAIEYGAIGVGLFRTEYLFMTGAELPDEERHYQTAVAVLDKLKGAQ
                     ATIRTFDLGSDKLARFIEQADLGEQNPALGLRSIRLCLSTLGRDLFRAQLRGLLRASA
                     HGPMKIMFPMISGVAELRAVKLVVEDVKKELTAEGVAFDPNIKLGIMIEMPSAALTAD
                     LLAQECDFFSIGTNDLIQYTMAVDRVNEYVSYLYEPLHPSLIRLIGEVAKAGKAANIP
                     VTICGEMAGEPMIAPVLFGLGIREFSMSAVSIPEVKATIRQMTVAQTEILVERVRNLP
                     TAAEVRAVVTDYVFGLATPKKEPV"
     gene            6498..9395
                     /locus_tag="VMZ53_14085"
     CDS             6498..9395
                     /locus_tag="VMZ53_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29637.1"
                     /translation="MRWLVAAALVAAAAGRVEAGDSSRVYKTVESEHFIVHYWEPLDD
                     VARRVAVVAERAHTVLTVSLDHVPDTKTIIYLTDDTDSANGFAGVLPRNEITLYATAP
                     SGFSELEDYDDWLYGLVAHEYTHILHIDTMEGLPRIYNKIFGKTWAPNQIMPRWIIEG
                     IATYEETKRTSGGRNRGTRFDEFIRVAERGGTDLTLDQISGAPRQYPRGNAAYVYGSH
                     FLNYVFDRFGDDALRKMSHTAGAFPPPFAINRQIAKVVGEPFTELYDDWKGYLRDKYG
                     MQEQAAARRGLRPGKQLTTTAESNLLPHYSADGKEIYWLFYDGYSLPRVRAMPVGSTL
                     TDVNDGARDIVQIDAMGPFDLLPDGSLVYEQGRQFRSVYGYEDIFRWDAKTGQTTRLT
                     TGRRAREPSVSPDGRKIAYSQNGRSESSLAVMDAVPNAPATIVWKGERYDQAFQPAWS
                     PDGTRIAFSAFLKGGFRDILVVDVATGAVERITHDRAVDLHPEWSSDGTLLFFASDRT
                     GISNIYAFDLAKRATWQLTNVIGSAFEPHPSPDGKRLSYIASVAKGGYDLFELALDDR
                     SAWQPARDYVDDRPPASNIPDASAVVSAPRPYRPFESLAPQSWTLSVDSASRTASIQT
                     SGSDSVGLHAWSLAVGLDQLGGVDVGGSYGYFGFLPALRFAGARTLLERGGWRIDGVN
                     KRYTEEDWSATLSVGIPFESRPSSSWTLSFDYDTDWFRLVKAPNFMDDPNNRAPVMPA
                     TDYFQAGIGTRIGFSTVRGTTFGLGSQSGFDGSVGIRIDDPLLGATYRNFTFSYAADR
                     FQRLAFLGDSPVLSVRVAGGLRAGDLVRLGGFGLGGVPAQDIVQGIVNSQRTGSSGYL
                     RGYPARTVAGNQYHLLNLEYRQALYYVERGLQTLPVYLRRVHMAVLADVGTAFDTTFE
                     PRRDFRASLGAALRLDAFFGYFVPGTFEVGYSHGLTSEGVHETWFLLTGSL"
     gene            9395..10051
                     /locus_tag="VMZ53_14090"
     CDS             9395..10051
                     /locus_tag="VMZ53_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29638.1"
                     /translation="MKPSDLAGDVAIEISRQVVDPLRGLRDRLGLIVDLIERHVATST
                     GPTPYPWRSLTTLRQDLAAAYLEATQLSRRLEELDRALSEEQPVAGFDLSAAVDLGLR
                     LAGHHMGTGIELLFDLGNVPPAKGVPGMLSLIVAQLVAVSARSAHVVAGSSLSVRVWQ
                     EEEWAVVTIADNGGGAADAEQLGELARSVVTEWGGTVDAAAAEGKGCAFELRLATIAS
                     "
     gene            10690..11037
                     /locus_tag="VMZ53_14095"
     CDS             10690..11037
                     /locus_tag="VMZ53_14095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUS29639.1"
                     /translation="MTTFAAYEVAIELIKELRPIVEIVGKHDSNLSDQMKRAGTSVVL
                     NLSEGARRQRGNQQRAYEIAHGEARELLGALDCAAAWGYVLDAANARRTLDRLLGLLW
                     GLTHPGRMRAPRA"
     gene            complement(11065..12513)
                     /locus_tag="VMZ53_14100"
     CDS             complement(11065..12513)
                     /locus_tag="VMZ53_14100"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HUS29640.1"
                     /translation="MRRLMLIALGFAVGCGDDTIRDPVNFPDAGPPDASTCTTRITYA
                     STWMKPDGHVGSTDVVDGVVEWDGTCTDDGANSYATLSNGWKPYFSGKQACAIALDYS
                     ECRVTGRCTTRITYASTWSHATTHPEQFDDVNARVFWDGACTNVASASYAKLSNAWQP
                     YFTGNNACGMSFRWTNCGGLYSNPVLPMDCPDPFVAGSVLTCTSGNAPNHFPLYTSDN
                     LITWQPIGHIFAGVRPAWAVSDFWAPEIHYVNGSYVAYFTARGTDGRLAIGVATSPSA
                     IGPFTPRPTPLVHSTSVGLIDATYFSDGTTGYLAWKEDGNAQNQPTPIRASALAPDGL
                     SLIGLTTQLITNDQTWEGNLVEAPEILVWQNSYYLFYSGNAYYDGRYAVGVAKASSPL
                     GPYTKYANNPILATSNEWVGPGHCSITAGPQVSTDEELANDLYMVYHAWQAGHVNGPG
                     DGRMVLVDQIQFGSDGWPHMFGAPSSASRPSP"
     gene            12601..13218
                     /locus_tag="VMZ53_14105"
     CDS             12601..13218
                     /locus_tag="VMZ53_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS29641.1"
                     /translation="MKSAARKRTYDSELRKQHAEHTAERILDAVKTLLGRGDRELAYT
                     SIAREARVAVPTVYRHFPTQDDLFRAFAVHEQKRYGEVTLDRASMPKLVRGFYSRFDD
                     PNDAFHKQQRLATIWRFSRVGTVPGRRVAFEKLIDAEAPGLAEPERTQIVDLAVVALS
                     SAMGEAMRGYLELDGEAMAARALLMIEALLRHARELTKAKGKGKS"
     gene            13289..15505
                     /locus_tag="VMZ53_14110"
     CDS             13289..15505
                     /locus_tag="VMZ53_14110"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HUS29642.1"
                     /translation="MRASGGTLVTFVVRLDHARARERARCGGKASALAEMIAAGFSVP
                     AGVVVTIDAFDDAGALRAEARDELRGELAALHGPFAVRSSGIAEDLVGASFAGQYETV
                     LDVEGFDAVVAAIATCVASVNAPRVAHYRRDHDIRETRMAVLVQELVRPRAAGVAFTA
                     HPVTGERDVVVVSATAGLGEALVSGERDGEEWDVRRDVAPIRRRGRERVLREGDARNI
                     AELARQVASASTGGDVPTDIEWALREDGSVALLQARPMTAVPEPVSWQSPAPGGYLRN
                     FRLGEWLGAPMTPLCRTWIVDDLERQLHAYFYELSGARMAAEPHVVVNGWYFYGGLNF
                     KMGFGALVRIIKTLLSKRRAQLLAAIPPVAHKGFDREVARWRDELLPALRKTVADAEA
                     RVERARPVELLAIVQSILDATAAQFQSVVGVAGYAAKAEGKLFALWRKHLREHDGAVL
                     DVVVGAQTSPAAHDVEGLDPIFPTLGERGALPASISDETRARVLARRDEAEARALEKL
                     KPRAKRIVAAAIAEARRAHAIRIEQTGLVTLGWPVLRRALLRLGEHAVEQGAIAQHDE
                     IFFVERAELEAVAAGRATHIDVEARRALWERQRKLVPPLVVGELTGLAKKIFAEIDQL
                     LHHAERDIPGAIVGMPGSPGRVTGPARIVRSVDELDRLQPGEVLIAPVTTPAWTLGFG
                     RAIAIVTDTGSIASHASIVAREHGIPAVVGTGTATAQLVDGALVTVDGGRGVVLRA"
     gene            complement(15506..16552)
                     /locus_tag="VMZ53_14115"
     CDS             complement(15506..16552)
                     /locus_tag="VMZ53_14115"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HUS29643.1"
                     /translation="MREWCWIALCIAACGPTPGHHGNGDDDTTGDDDTTVDALVNLYN
                     DAAIDSQCGAQMQNIGVVNLGDPPDLLVVLDRSGSMSESPPVFPPVFDTKWNIMKGAL
                     NNVAAQKDQNIKFGLLEFPSDQNCAADATAEVSVALGTHSQIATYFAGRSPGGNTPAH
                     VALSSALTYFNSIPVNTAGRYVLFATDGLPNCQGGDPNAASDAATVAAVTALKTAGIP
                     TYVIGFGTFGLNTGVLNDAAVAGGKAKPGNTKFYEANNAQDLANALQAIAGGIIVPSC
                     TFQLQSAPPDPANVTVTVNGTPVPKDASHANGWDYYPNAMTITFFGSYCQQVQMGASN
                     AVSFIYGCPGPILQ"
     gene            complement(16632..>17238)
                     /locus_tag="VMZ53_14120"
     CDS             complement(16632..>17238)
                     /locus_tag="VMZ53_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29644.1"
                     /translation="ASGLATAGVAAAALSLVPGGVFACAGLAYLAHERFSEARRRRRS
                     RDLLLALGELEVALHVADGPEAAKGIADQLAPYTRDAPITRPEVYEDAKRRIRAQAEG
                     RGEASARRELEHALLVGSDVVFLRNDYLQVGQVAFKIGEVREYALRGANLPLTGGRLL
                     QAAMGLLVVEAEERAAAGEDIALLSKRVADYEAWSGHTAGR"
BASE COUNT         3012 a         5372 c         5960 g         2894 t
ORIGIN      
        1 tcgagaacac cggcagcggc agcgcgaacg tcgtccagaa caatggcagc gcgggcagcg
       61 cggttggcgt tgtggcgacg ggcagcgata ccggcagcgc cggcacgatg acgggcagcg
      121 acaacggcag cgcaaccacg atggtgggca gcggcgcggg caagccggtc gacaccgtga
      181 tcgcatcggc cgcaaagggc gcgatggtcg agatcgaagg cacggacatg aagggtcctg
      241 cgccattcac cgccaagctc gagaaggacc gcgcatacaa ggcacgcgtc accgcgccgg
      301 gctttgtcgc gcaagagatc gaagtgaagg gcggcgctcc gaaggtcact gcgaagctcg
      361 ccgcgaagtc gaagatcatc tcggtcacga ccgatccgcc gggcgccgac atcctcgtcg
      421 atggcgtgcc gacgtcgaag ctcacgcctt cggacgtgca gctcacggcg gcgcaatcgg
      481 cacgcgctcg cgtgcgcatc acgctgcgcc gtcccggcta caagcagcag gatcaatacg
      541 tcgagaccgc cgcgtggaac gaaggcgaca gctcgatgac cgccagcgtg accgcgaagc
      601 tcgtcgtcgc gccgaaggtc gtgccgaaca acaacggcaa tacgacgacg aacaccggct
      661 cgggctcggg ctcggctggt actggcgacg gctcggcgac gacgccgacg ggtggcggca
      721 ccggctctgc gtcgggcacg acgacgacgc ctccaacagg cggcacgacg acgccaccga
      781 ccggtggaac gacgacacct ccggccggcg gaacgaccac gcctccgagc ggtggcacga
      841 ctcctccgag cggcggaacg acgccacccg cgacgggctc cgcctcgggc ggtcagacac
      901 tcaagtagct cgcgatgcgc gtcgtcctct tggcactgct cctcgcagcg gcctgcaaag
      961 agaaggacga caagaagagc gcgaagcccg tcgtcgtaga tccgcggcct ctcgcgcggg
     1021 gatcctcgaa tgggtcggcg acaccaacac ccgtcgccgt cgaagcgcac aaggcaccgg
     1081 cggcaccgat gcagatcgaa gacgcgcgtg ccgtgctgcc gaagatcgaa ggcaacgtga
     1141 tcctcgatct caagcagacg agcgacaagc ggcaggtgca tgcgacgtgg tgcatcgatg
     1201 gcaccggcgc cgacgaagtc gcgaagctcg tcggcaagtg gatgtccgat gcgggctaca
     1261 ccgcgctatc gattcgcggc gacgcgcgca aagcaggcgt ttccggtgac cgcggcgaga
     1321 tccggctctc gatggttgtc gcgtcgtcgg gtgccgcgaa ctgtcccgca ccaacgcatt
     1381 acttcgcgag cgcgacgatc ttcagaccct aggcgaccct aggtgggatg aggtagactc
     1441 gcggcgtccg cgaatttctc gtcgaggatc gctaggatct tccgagcgca tcggcggtat
     1501 gatcgaaggc gaaccgattt gggaggccct cgatgcaatt cgcggtgacg ttccgccata
     1561 tggaaccgac cgatgctctg aagtcctatg cacgcgagcg aatggagcga gttcgaaagt
     1621 acctgcctga tccgatcgcc tgtcacgttg tcttgtcgac cgaacgtcac aaccaccgca
     1681 tcgacgtcaa cttccagttg cacaacggcc tgtcgatcgc cggccacgaa acgaccgaga
     1741 acatgtacag ctcgatcgat ctctgcatcg cgaagatcga gcgtcaggtc cggaagtaca
     1801 aaggcaagct cgaaggcatg cgcgcgcgcc cgcatcacgt ggtcgcgttg ccttggtcac
     1861 actcgatcat cgatgaagcg tttcacgagc tgaaagacga cggtgcgaac ggtcacgctg
     1921 cgacgaagcc ggcacccgag ttcaccgtcg tcaagactga gaagtttcat gcacagccga
     1981 tgtcggtctc cgacgcgatc gtgcagctga accttctgca tgagcccttc ctcgtgttcc
     2041 gtgcggacac cgacggtcga gtggccgtgg tctacaagcg tgaggacggc agctacggtc
     2101 tcatcgaaac gccaacagca tcttcataga aatttacatg tggtagggtc gatttggccc
     2161 tagatgaaga tcgtcgactt gatcaagcgc gacatggtcg tccccgcgct gcaggcgacc
     2221 gacaaacgcg gcatcctcga ggagctcgcc gcgtacatgt ccgcgcatca cacgcggatc
     2281 gatcgcgctt cgctctcgaa ggtgttgatc gagcgcgagc aactcgcatc gacggcgatc
     2341 ggcgagggcg tcgcgattcc gcacggcaag ctcggcgcgg tgggcgagat cgtggcgtgt
     2401 ttgggccgtg caccgaccgg cgtcgagttc gactcgatgg atggccagcc gacgtttttg
     2461 ttcttcgtgc tcgtcgcgcc cgagtcatcg accggtgccc acttgaaggc actcgcgcgg
     2521 atcagccgcg tgttcaagga cccggagttc cgtcgccgcc tcatggctgc aggggacgcc
     2581 gaatcgatgt accacgtggt agccgacgag gacgccaagt actagatgtc gggttcgatc
     2641 acggtcggcg agttgctcga acgggccgag ctcggcgtga ccacggttgc cggggcgagt
     2701 ggccttggcc gcacggtgat cgtgccgcgc attcagaagc ccggtctcgc actgacggga
     2761 tggaaagagc agctgcacag caatcgcgtg ctcgtgctcg gcggaaccga aatcgagtac
     2821 ctcgcggatc acgaagaagc gcgcaagaac ggcatcgcga cgatgctcgc gagcgatcca
     2881 gcgtgtgtcg tgatctgtcg cgggctggac gcaccggacg agctcgtcca cgcgtgcgaa
     2941 gagcgcggcg ttccgctcgt ccaatcgacg ctcgtgacgg cggacttcat tcatgcggtg
     3001 acggcgtgga tggccgaccg gctcgcgccc tcgacggagc gccacggcgt gctcatggac
     3061 gtgctcggca tcggcgtgct gatgacgggc aagagcggca tcggcaagag cgagacagcg
     3121 ctcgatctcg tgatgcgtgg gcatcggctc gtcggggacg acgtgatccg cattcgccgg
     3181 caaggcagcg cgctgagcgg acgcggtgcc ggcatcctcg gccaccacat ggagatccgc
     3241 ggtctcggaa tcatcaacat caaagatctg ttcggcatct ccgccgtgcg cgaagccaag
     3301 gtcatcgacc tcatcgtcga gctccacgag tgggatgccg atgccgagta cgaccggctc
     3361 ggattcgacg atcaaacgga gcgtttgctc gactacccgg tgccaaagct gcggctgccc
     3421 gtgcggccgg gtcgcaacct cgcgacgctg atcgaagtcg ccgcgcgcaa ccagctgctc
     3481 aagcagcagg gcatccactc cgcgaaagca ttccgcgaca agctgcaccg cgcgatgggc
     3541 tccgccggta gtccaagtga attggactcc ctccatcatc ggaacgacgg tccaggggcg
     3601 accgagtaaa tgcagatcgt gatcgtcacg gggttgtcgg gtgctggaaa gagcaccgcg
     3661 ctgcgtgcgc tcgaggacat cgagttcacg acggtcgaca acattcccgt gccgctcgtc
     3721 ggtcagctcg tcgagcaact cgcgcacgaa ggtgatcgcg acaaggtcgc tatcgcgatc
     3781 gacgcgcgcc agcgccgcaa cctcggcatg tgggcacaga tgctcgcgct cctacgcggc
     3841 gcgggtcacc gcctcgaggt gatgttcctc gacgcgaacg acgaggtact cctccgccgg
     3901 ttctcggaaa cgcgacggcg acatccactc tccggcgacg atctgatcga gggcgtgcgg
     3961 cgcgatcgcg aggtgatgac ggagatgcgc cacggcgcgg ctgtcgtcga cacctcgagc
     4021 ttcaacatgc atcagttgaa gaagatcatc caagagcgtt acggcggcac cgggcgtctt
     4081 gcggtgacga tcgtgtcgtt cggcttcaag cacggtctgc cgctcgaatt caatctagtc
     4141 ttcgacgtgc ggtttctgcc gaacccattc ttcgaccccg cgctgacaca cctgaccggt
     4201 cgcgatcccg aggtcgtgaa gttcgtgatg gggcccgaag gcacgcagta cctcgagcac
     4261 atcgagtcga tgctgcggtt tgcgctaccc cagttccaaa aagagggaaa gctgtacgtc
     4321 accgtgtgcg tcggttgcac gggtggccgg catcgctcgg ttgcagtcgt cgaggagctg
     4381 cgtcgccggc tcggccagga gtgggatatc ctgattcgtc atcgcgacgt ggagcgaggc
     4441 gagtaatggc ggctgtggcg gaaaagacgc tgaagattca gaacgagctc ggcttgcacg
     4501 cgcgcgcagc gacgaagctc gtgcagctcg cctcgaagta tccgtgcgag atcaccgtta
     4561 cgaaagacgg caacgaggtc aacggcaaga gcatcatggg cgtgctcatg ctcgtcgcga
     4621 gcaaaggcac gacggtcacg gtgcgggcga agggcgataa ggcggacgag gccgtcgccg
     4681 cgatcgcggc gctgatcgac gacaagttcg gcgaaggaaa atgacgcgat gacggccgca
     4741 cgcgaggagg tccgcaagac gggtctcggc gtctcggccg ggattgcgtt cggtcgcgcg
     4801 tatctcatcg gccgcgacac gctcaaggcg ccgcgtcatc acatcgaagc tgacgatgtc
     4861 gacaccgaag tcgcgcgtct ctacaaagcg atcgcggcga gcgacaagca gctcgcgaag
     4921 atcaaagaga agctcgcgag cgagaacgag agcgactacc acatcatcac cgcgcaccag
     4981 atgatgctcc acgacgagca cctcgtcggg gccgccgtcg agtacatgcg cgaggagatg
     5041 gtcaacgccg agtgggcatt gcgccgcgcg gtcgacgaca tccgcagcgt gttcgacagc
     5101 atcgaggatc cctatctgcg cgagcggcgc agcgacgtcg agttcgtgtt cgagcgcgtg
     5161 ctgcgcaacc tgctcggccg cgatatgggc ccgctgtcgc cgccacccga tgcgatcgtc
     5221 gtcgcgtacg acctgtcgcc cgcggatacc gcgacgctgc acaaagcggc ggtcgccggg
     5281 ctcctcaccg atgccggcgg taagacgtcg cacaccgcga tcattgcgcg cgcgcatgag
     5341 attccggcgg tcgtcggcct cgagaacatc accgagctcg tcgagacgga tgacctgctg
     5401 ctgctcgatg gcggcacggg catcgtgatc gtcaatccgt cggcagcgac ggtcgcgcag
     5461 tatcgtgacg agcagcgccg gcaagtcgcg gagggcgtgc gcttccacgc gatgcgcgat
     5521 ttgcccgcgc gcacgcgaga cgacctcgat gtcgcgctgc tcgcgaatat cgacggcccc
     5581 gacgagctcg acgacgcgat cgaatacggc gcgatcggtg tcggcctgtt ccggaccgag
     5641 tacctcttca tgacgggtgc cgagctgccc gacgaggaac gccactacca gacggcggtc
     5701 gcggtcctcg acaagctcaa aggtgcgcag gcgacgatcc gcacgttcga cctcggcagc
     5761 gacaagctcg ctcgcttcat cgagcaagcc gacctcggcg agcagaatcc ggcgctcggc
     5821 ctgcgctcga ttcgcctgtg cctctcgacg ctcggccgcg atctgtttcg cgcgcagctt
     5881 cgcggcctgc tccgcgcgtc cgcgcatggc ccgatgaaga tcatgttccc gatgatctcg
     5941 ggcgtcgccg agctgcgcgc cgtgaagctc gtcgtcgagg acgtcaagaa ggagctgacg
     6001 gccgaaggcg tcgcgttcga tccgaacatc aagctcggga tcatgatcga gatgccgtcg
     6061 gccgcgctca ccgcggacct cctcgcccag gagtgcgact tcttctccat cggcacgaac
     6121 gacctcatcc agtacacgat ggcggtcgat cgcgtgaacg agtacgtctc gtatctctac
     6181 gagccgctgc atccgtcgct gattcgcttg atcggcgagg tcgcgaaagc agggaaggcc
     6241 gcgaacattc cagtcacgat ctgcggcgag atggcgggcg agccgatgat cgcgcccgtg
     6301 ctgttcggcc tcggcattcg cgagttctcg atgagcgcgg tgtcgattcc tgaagtgaag
     6361 gcgacgatcc ggcagatgac ggtcgcgcag acggagatcc tcgtcgagcg agtgcgcaac
     6421 ttgccgaccg ccgcagaggt tcgcgccgtc gtcacggatt acgtgtttgg gctcgcaacg
     6481 ccgaagaagg agcccgtgtg aggtggctcg tggcggcggc gctcgtcgct gctgctgcgg
     6541 gaagggtaga ggcgggtgac tcgtcgcgcg tctacaagac cgtcgagagc gagcacttca
     6601 tcgttcacta ctgggagccg ctagacgacg tcgctcgtcg cgtggccgtc gtcgcggagc
     6661 gcgcgcatac ggtgctcacc gtctcgctcg atcacgtgcc cgacacgaag acgatcattt
     6721 atctcaccga cgacaccgat agcgcgaacg gctttgcggg cgtgctgcct cgcaacgaga
     6781 tcacgctcta cgccacggca ccctcgggat tctcggagct cgaagactac gacgactggc
     6841 tgtacggcct cgtcgcgcac gagtacacgc acatcctcca catcgacacg atggagggcc
     6901 tgccacgcat ctacaacaag atcttcggca agacctgggc gccaaaccag atcatgccgc
     6961 gctggatcat cgaaggcatc gcgacgtacg aagagacgaa gcgaacgtcg ggtgggcgca
     7021 accgaggcac gcgattcgac gagttcattc gcgtggccga gcgtggcggc accgacctca
     7081 cgctcgatca gatcagcggg gcgccgcgcc aatacccgcg tggcaatgcg gcgtacgtgt
     7141 acggctcgca ctttctcaat tacgtcttcg atcgcttcgg tgacgacgcg ctccgcaaga
     7201 tgtcgcacac cgccggcgcg ttcccgccgc cgttcgcgat caaccgccag atcgcgaagg
     7261 ttgtcggcga gccgttcacc gagctgtacg acgattggaa aggctacctc cgcgacaagt
     7321 acggcatgca agagcaggcg gccgcgcgtc gaggattgcg gccgggcaag cagctcacga
     7381 cgaccgcaga gtcgaacctg ctgccgcatt actcggccga cggcaaagag atctactggc
     7441 tcttctacga cggctactcg ctgccgcgcg tgcgtgcgat gccggtgggc tcgacgctga
     7501 cggacgtcaa cgacggcgcc cgcgacatcg tgcagatcga tgcgatgggc ccgttcgatc
     7561 tgcttcccga cggctcgctc gtctacgaac aaggccgcca gttccgctcg gtgtacgggt
     7621 acgaggacat ctttcggtgg gacgcgaaaa cggggcagac gacgcgcctc acgaccggcc
     7681 gccgcgcgcg cgagccgagc gtgtcgcccg acggccgcaa gatcgcgtac tcgcaaaacg
     7741 gtcgctccga atcctcgctc gccgtcatgg atgcagtgcc gaacgcgccg gcgacgatcg
     7801 tctggaaagg cgagcgctac gaccaagcct ttcagccagc gtggtccccg gatggcacgc
     7861 gcatcgcgtt ctccgcattt ctgaaaggcg gcttccgcga catcctggtc gtcgacgttg
     7921 cgaccggcgc cgtcgaacgc atcacgcacg accgcgcggt cgacctgcat cccgagtggt
     7981 cgtccgacgg cacgctcctg ttcttcgcga gcgaccgcac cggcatctcc aacatctacg
     8041 cattcgatct cgcgaagcgc gcgacgtggc agctgacgaa cgtcatcggc agcgcattcg
     8101 agccgcaccc atcgcccgac ggcaagcgcc tctcgtacat cgcatccgtc gccaaaggcg
     8161 gctacgacct gttcgagctc gcgctcgacg atcggtccgc gtggcaaccc gcgcgcgact
     8221 acgtcgatga tcggccgccc gcatccaaca ttcccgacgc gagcgcggtg gtttcggcgc
     8281 cgcgtccata tcgccccttc gaatcgctcg cgccgcagag ctggactctg tccgtcgaca
     8341 gcgcgtcgcg caccgcctcg atccagacgt cgggctccga ctccgtcggc ctgcacgcgt
     8401 ggtcgctcgc tgtcggcctc gaccaactgg gcggcgtcga cgtcggcggg tcgtacggct
     8461 acttcggctt cctacccgca cttcgcttcg cgggcgcacg cacgctcctc gagcgcggcg
     8521 gttggcgcat cgacggcgtg aacaagcgct acacggaaga agactggtcg gcgacgctgt
     8581 ccgtcggcat tccgttcgaa tcgcggccgt cgtcgagctg gaccctctcg ttcgattacg
     8641 acaccgactg gttccgcctc gtgaaggcgc ccaacttcat ggacgatccg aacaaccgcg
     8701 cgcccgtgat gccggctacc gactacttcc aggcaggcat cggcacgcgc atcggcttct
     8761 cgaccgttcg cggcacgacg ttcggcctcg gctcgcagtc aggcttcgat ggctccgtcg
     8821 gcatccgcat cgacgatcct ctcctcggcg cgacgtatcg caacttcacg ttcagctacg
     8881 cggccgatcg cttccagcgc ctcgcattcc tcggcgactc gcccgtcctc tcggtccgcg
     8941 tcgccggtgg cctgcgcgcg ggtgatctcg ttcgcctcgg cggcttcggc ctcggtggcg
     9001 tgccggccca agacatcgtc caaggcatcg tcaactcgca gcgcacgggc tcgtcgggat
     9061 acctgcgcgg ctatccggct cgcaccgtcg cgggaaacca gtaccacctg ctcaatctgg
     9121 aataccgcca agcgctctac tacgtcgagc gcggcctgca aacgctgccg gtctacctgc
     9181 gccgcgtcca catggcggtc ctcgcggacg tcggcaccgc gttcgacacg acgttcgagc
     9241 cgcgccgcga ttttcgcgcc tcactcggcg ctgccttgcg tctcgacgcc ttcttcggct
     9301 acttcgttcc aggaaccttc gaagtcggct actcgcacgg cctcacctcg gaaggtgtcc
     9361 acgaaacctg gttcttgctg acgggaagtc tgtagtgaag ccaagcgatc tagctggaga
     9421 cgtcgcgatc gaaatctcgc gacaggtcgt cgatccgctg cgtggtctgc gagaccgcct
     9481 cggcctgatc gtcgatctga tcgaacgaca cgttgcgacg tcgacgggac cgacgccata
     9541 tccctggcgc tcgctcacga cgctgcggca agatctcgcg gctgcatatc tcgaagcgac
     9601 gcagctgtcg cgccggctcg aagagctcga tcgcgcgcta tccgaagagc agccggttgc
     9661 gggattcgat ctctcggctg cggtggactt agggcttcgg ctcgcgggcc atcatatggg
     9721 gacggggatc gagctgctct tcgatctcgg caacgtgccg ccggcgaagg gagtaccggg
     9781 gatgctgtcg ctgatcgtcg cgcagctcgt agcggtgtcg gcgcgttcgg cgcacgtcgt
     9841 cgcgggctcg tcgctgtcgg tgcgcgtgtg gcaggaagag gagtgggcgg tcgtcacgat
     9901 cgcggataat gggggagggg cggcggatgc ggagcagctg ggagagctgg cgcggtcggt
     9961 ggtgacggag tggggaggaa cggtggatgc ggcagcagcg gaagggaaag gctgtgcgtt
    10021 cgagctgcgg ctggcgacga tagcgtcgta gcggcttcgc gggcggtggc cgtcgcggaa
    10081 gcggtatcgg ccgcggaagc cgtctcggtc tcggtatcgg aagcggtatc ggtatcggaa
    10141 gcggtatccg aagcggaagc ggtatccgaa gcggaagcgg tatcggtatc ggtatcggta
    10201 tcggtatcgg aagcggaagc ggaagcggaa gcggaagcgg aagcggaagc ggtagcggta
    10261 tcggaagcgg tatcggaagc ggaagcggta gcggaatcgg aagcggaagc ggaatcggaa
    10321 gcggtatcgg aagcggtatc ggaagcggta gcgggtgtcc ggaacccgga cagtaatgct
    10381 acggcgccct cggcgacggg gcgcgggttc ggcggcgctc tcgctgcgta ctcgctccgg
    10441 cacgagacga tccaagcgcc cgatcgcggg tgcagatcgc gaacgcgggc cgctcggacc
    10501 cacgtgggtc gtgaacacgc acgtggggcg cgagcgcttg gatcgcctcc tgcctccgct
    10561 cgcacgcgac gcgaagagcg ccgccgcccc cgctgccccg ccgcctcggg agggagggcc
    10621 ccagtcctag gggagccgga gcaagcgagt cgcaaagcgg gtgctcacca cccagaaacg
    10681 gagaacatca tgaccacgtt tgctgcatat gaagtcgcta tcgagctgat caaagagctg
    10741 cgtccgattg tcgagatcgt ggggaagcac gattcgaacc tgtcggatca gatgaagagg
    10801 gctgggacga gcgtggtgct caacctcagc gagggcgcgc gtcgtcagcg cggcaatcag
    10861 cagcgcgcgt acgagatcgc gcacggcgag gcgcgcgagc tactcggtgc gctcgattgc
    10921 gcggcggcgt ggggctacgt gctggatgct gcgaatgcgc gccgcacact cgatcgcctg
    10981 cttggcttgc tgtggggcct cacgcatccg ggacgcatgc gcgcgccacg cgcgtgattt
    11041 ctgtgcccac gtgggcacgc aatctcacgg cgaaggacgc gaagcgctcg acggcgcgcc
    11101 gaacatgtgc ggccagccgt cggatccgaa ctggatctga tccacgagga ccatgcgtcc
    11161 gtcgccagga ccgttgacat ggccggcctg ccacgcgtga tagaccatgt agaggtcgtt
    11221 cgcgagctcc tcgtccgtcg acacctgcgg tcctgccgta atggaacagt ggcccgggcc
    11281 gacccactcg ttgctcgtcg cgaggatggg gttgttcgcg tacttcgtgt acgggccgag
    11341 aggcgagctc gccttcgcga cgccgaccgc gtatctaccg tcgtagtacg cgttgccgct
    11401 gtagaagagg taatagctgt tctgccagac gagaatctcg ggcgcctcga cgaggttgcc
    11461 ttcccacgtc tgatcgttcg tgatgagctg cgtcgtcaga ccgatgagcg acaagccgtc
    11521 gggagcgagt gcgctcgcgc ggattggcgt gggctgattc tgagcgttgc cgtcttcctt
    11581 ccacgcgaga tagccagttg tgccatcgct aaaatatgtc gcgtcgatca atccgacgga
    11641 cgttgagtgc acgagcggag tggggcgcgg cgtgaacggg ccgatggccg acggcgacgt
    11701 tgccactccg atcgcgagcc ttccgtcagt gcctcgcgcg gtgaaatacg cgacgtagct
    11761 gccgttgacg tagtggatct cgggagccca gaagtccgat accgcccacg cgggtcgaac
    11821 gcccgcgaag atgtgcccga ttggttgcca cgtgatgagg ttgtcggacg tgtacagcgg
    11881 gaagtggttg ggagcattgc ccgacgtgca cgtgagaacg ctgccggcga caaacggatc
    11941 cggacagtcc atcggcagga ccgggttcga gtagagaccg ccgcaattcg tccagcggaa
    12001 tgacatgccg cacgcattgt tgccggtgaa gtatggctgc cacgcgttcg agagcttcgc
    12061 gtacgaggcg ctcgcgacat tcgtgcatgc tccgtcccag aacacgcgcg cgttcacgtc
    12121 atcgaattgc tccgggtggg tggttgcgtg tgaccacgtc gaggcatatg taatgcgcgt
    12181 cgtgcagcgt ccggtgacgc gacactcgga gtaatcgagc gcgatcgcgc atgcctgctt
    12241 gccactgaag tacggcttcc atccattgga caatgtcgcg tacgaattcg cgccatcgtc
    12301 ggtgcaggtg ccgtcccact cgacgacgcc gtccacgaca tcggtgcttc cgacgtggcc
    12361 gtcaggcttc atccacgttg acgcgtacgt gatgcgcgtg gtgcacgtcg atgcgtcagg
    12421 cgggccggca tcggggaagt taacggggtc gcggatcgtg tcgtcgccgc aaccaacggc
    12481 gaacccgagc gcgatcagca tgaggcgacg caccgctgca gcgtacaccg ccggcttacc
    12541 gagaaaatca gcttgtgttt cgtgttatac aggtgataca ttaattaacg ctgcacacac
    12601 atgaaatccg ccgcacgcaa acgtacgtac gacagcgagc tccgcaagca gcacgccgag
    12661 cacaccgccg agcgaatcct cgacgcggtg aagacactgc ttggaagagg cgatcgcgag
    12721 ctcgcgtata cgtcgatcgc gcgagaggcg cgcgtcgcgg tgccgaccgt gtaccggcac
    12781 tttccgacgc aagacgacct gtttcgcgca ttcgccgtcc acgagcaaaa gcgctatggc
    12841 gaagtgacgc tcgatcgcgc gtcgatgccc aagctcgtgc gcggctttta tagccggttc
    12901 gacgatccca atgacgcgtt ccacaagcag cagcgacttg cgaccatctg gcggttcagc
    12961 cgcgtcggta cggtgccggg gaggcgggtc gcattcgaga agctgattga cgccgaagct
    13021 ccggggctgg ccgagcccga gcggacgcag atcgtcgact tggcggtcgt tgcgctatcg
    13081 agcgcgatgg gagaggcgat gcgcgggtac ctggagctgg atggtgaggc gatggctgcg
    13141 cgagcgttgc tgatgatcga ggcgttgctg cggcatgcgc gcgagctgac gaaggcgaaa
    13201 gggaagggaa agtcatgaag aggcgcgtga gttgggtggc ggatgcgggc ggcggcgtgc
    13261 gcggggcggg gaaggtgttc gaggatctgt gcgggcgagt ggaggcacgc tcgtgacctt
    13321 tgtggtgcga ctcgatcatg cgcgtgctcg cgagcgtgcg cggtgtggcg gcaaggcgag
    13381 cgcgttggcg gaaatgatcg ccgcgggctt tagcgtgcca gcgggcgtcg tcgttacgat
    13441 cgatgcgttc gacgatgcgg gggcgctgcg cgcggaggcg cgtgacgagc tgcgcggcga
    13501 gctcgcggcg ttgcatgggc cgtttgccgt tcggtcgtcg ggcattgcgg aggatttggt
    13561 gggcgcgtcg ttcgcggggc agtacgagac ggtgctcgac gtcgagggct tcgatgcggt
    13621 cgttgcggcg atcgcgacgt gtgtggcgtc ggtgaatgcg ccgcgcgtcg cgcactatcg
    13681 acgcgaccac gatattcgcg aaacgcgcat ggctgtcctc gtgcaagagc tcgtgcggcc
    13741 gcgcgcggcg ggcgtagcgt tcaccgcgca tccggtgacc ggcgagcgcg acgtcgtggt
    13801 ggtcagcgcg acggctggat tgggcgaagc gctggtgtcg ggagagcgcg acggcgagga
    13861 gtgggacgtg cggcgtgatg tcgcgccgat ccgccgacgc ggacgagagc gcgttctgcg
    13921 tgagggagat gcgcgcaaca tcgcggagct cgcgcggcag gtcgcgagcg cgagcaccgg
    13981 cggtgacgtg ccgacggaca tcgagtgggc gctgcgcgag gacggcagcg ttgcgctctt
    14041 gcaggcgcgg ccgatgaccg cggtgccgga gcctgtgagc tggcagtcgc cggcgcccgg
    14101 cgggtacctg cgcaactttc gcctcggcga gtggctcggc gcgccgatga cgccgctttg
    14161 ccggacgtgg atcgtcgacg atctcgagcg gcagctgcac gcgtacttct acgagctcag
    14221 cggcgcacga atggccgcgg agccgcacgt cgtcgtgaac ggttggtact tctacggtgg
    14281 cctcaacttc aagatgggct tcggggcgct cgttcggatc atcaagacgc tcctgtcgaa
    14341 gcggcgtgcg caactgctcg cggcgattcc gccggtcgcg cacaagggct tcgatcgtga
    14401 ggtggctcgc tggcgtgacg agctgttgcc cgcgctgcgg aagacggttg cggatgcgga
    14461 ggcgcgcgtc gagcgtgcga gaccggtgga gctcctcgcg atcgtgcagt cgatcctgga
    14521 cgcgacggcg gcgcaatttc agtcggtcgt cggtgtggcc ggttatgcgg cgaaggccga
    14581 gggcaagctc ttcgcgctct ggcgcaagca tctgcgcgag cacgacggcg cggtgctcga
    14641 cgtcgtcgtc ggcgcgcaga cgtcgccggc tgcgcacgat gtcgagggcc tcgacccgat
    14701 ctttccgacg ctcggcgagc gcggcgcgct accagcgtcg atctccgacg agacgcgtgc
    14761 gcgcgtgctg gcccgacgcg acgaagccga ggcacgtgcg ctcgagaagc tgaagccgcg
    14821 tgcgaagcgc atcgtcgcgg cggcgattgc cgaggcacgg cgtgcgcacg caattcgcat
    14881 cgagcagacc gggctcgtca cgctcgggtg gccggtgttg cggcgcgcgc tgctacggct
    14941 tggcgagcat gcggtggagc agggtgcgat cgcgcagcac gacgagatct tcttcgtaga
    15001 gcgcgccgag ctcgaggcag ttgcggccgg cagagcgacg cacatcgatg tcgaggcacg
    15061 gcgagcgttg tgggagcgcc agcgcaagct tgttccaccg ctcgtcgtcg gggagctgac
    15121 agggctcgcg aagaagatct tcgccgagat cgatcagctg ctgcaccacg cagagcgcga
    15181 tatccctggc gcgatcgtcg gcatgccggg aagcccgggg cgcgtaacag gacctgcgcg
    15241 catcgtccgc agtgtcgacg agctcgatcg cctgcaacct ggcgaggtgc tcatcgcgcc
    15301 cgtgacgacg cccgcctgga cactcggctt tggtcgcgcg atcgcgatcg tcaccgacac
    15361 cggcagcatc gcgtcgcatg cgagcatcgt cgcgcgcgag cacggaattc ctgcggtcgt
    15421 cggcacgggg acggcgacgg cgcagctcgt cgatggcgcg ctcgtcaccg tcgacggcgg
    15481 acgcggcgtc gtcctgcgtg cgtgattatt gaaggatcgg gccggggcag ccgtagatga
    15541 agctgaccgc gttcgacgcg cccatctgca cttgttggca gtagctgccg aagaacgtga
    15601 tcgtcatcgc gttcgggtag tagtcccaac cgttggcgtg cgacgcgtct ttggggacgg
    15661 gcgtgccgtt gacggtgacc gtgacgttcg ccggatcggg cggcgcgctc tgcagctgga
    15721 acgtgcacga cggcacgatg atgccgcccg cgatcgcttg gagggcgttc gctagatctt
    15781 gggcgttatt tgcttcgtag aactttgtgt tgcccggttt ggctttgccg cccgcgacgg
    15841 cggcgtcgtt gagcacgccc gtgttgaggc cgaaggtgcc gaagccgatg acgtacgtcg
    15901 ggatgcctgc ggtcttcagc gcggtgactg cggcgacggt ggctgcgtcg gatgcggcgt
    15961 tgggatcgcc gccttggcag ttgggaaggc cgtcggtcgc gaacaggacg tagcgacctg
    16021 cggtgttgac cgggatcgag ttgaagtagg tgagggcgga ggagagggcg acgtgcgcgg
    16081 gcgtgttgcc gcctggcgag cggcctgcga agtaggtcgc gatttgggag tgtgtgccga
    16141 gcgcgacgga gacttcggcg gttgcgtcgg cggcgcagtt ttgatcgctg gggaactcga
    16201 gcaggccgaa cttgatgttc tggtcctttt gcgcggcgac gttgttgagt gcgcccttca
    16261 tgatgttcca cttcgtgtcg aacaccggcg ggaagacggg cggcgattcg gacatcgagc
    16321 cggaacgatc gaggacgacg agcaggtccg gcggatcgcc gagattgacg acgccgatgt
    16381 tttgcatctg cgcgccgcac tgcgagtcga tcgcagcgtc gttgtagagg ttcacgagtg
    16441 cgtcgacggt cgtatcgtcg tcgccggtgg tgtcgtcgtc accattgccg tggtgaccgg
    16501 gcgttggacc acacgcagcg atgcagagcg caatccagca ccattccctc atgcccgacg
    16561 atggacacga gatggcgtgc atgctcaacg ccagttagcg catgcgcgaa tcgtgaagcg
    16621 atctgcacta tctatcggcc ggcggtgtga ccggaccacg cctcgtaatc ggcaacgcgc
    16681 ttcgagagca gggcgatgtc ttcgcccgcg gcggcgcgtt cctcggcttc gacgacgagg
    16741 aggcccatcg cggcttggag gaggcggccc ccggtgagcg gcaggttggc accgcgcagc
    16801 gcgtattcgc ggacctcgcc gatcttgaag gcgacctggc cgacttgcag gtagtcgttg
    16861 cgcaggaaga ccacgtcgct gccgacgagg agcgcatgct cgagctcgcg acgggcggag
    16921 gcttcaccgc ggccttcggc ttgggcgcga atgcggcgct tcgcgtcttc gtagacttcg
    16981 ggccgcgtga tcggggcgtc gcgcgtgtag ggcgcgagtt ggtcggcgat ccctttggcg
    17041 gcttcggggc cgtccgcaac gtggagggcg acttcgagct cgccgagcgc gagcagcaga
    17101 tcgcggctgc gccggcggcg gcgtgcttcg ctgaagcgct cgtgcgcgag atacgcgagg
    17161 ccggcgcagg cgaacacgcc gccgggaacg agggaaaggg cggcagcggc gacgcctgcg
    17221 gtggcgaggc ccgatgcg
//
LOCUS       DATLBZ010000269         5764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9235507, whole genome shotgun sequence.
ACCESSION   DATLBZ010000269 DATLBZ010000000
VERSION     DATLBZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9235507"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VMZ53_14125"
     CDS             <1..818
                     /locus_tag="VMZ53_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29645.1"
                     /translation="HADEPRVAADVEPLAVERVVPGEACDDAVIDPVVTPMRDAYLDA
                     QRSACLRDELSAGLDVHALIDTPGFHGVLGGDLALGGRMIVKQAHELSVHVRVVDYAF
                     VQNAVNKVTNTGLGPVVLGAAAGKAMSETARAALVLRVELPFTRDNMDTMRSAAEIGG
                     VMTGKLSSSVTLNARLSALGMHASSLAGSTSRLAFRAGTDVVWQPRRTIGLQAGAELQ
                     AGWTRGFDAVLLRAGIHWAIHGGPWRFRSGIGAPIGGTERTNAIVDIAVLHDL"
     gene            870..1226
                     /locus_tag="VMZ53_14130"
     CDS             870..1226
                     /locus_tag="VMZ53_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29646.1"
                     /translation="MPRADDAKTRLTALAARVESDPAALADRAKGDVLAHEVGGEAAL
                     RWRIAVIRALIANPPDSDAVREAYGELVDRYRDDAKALSSIKSIGDEIRRRESEGSLA
                     STMVARTPRPRKQTKS"
     gene            1223..1624
                     /locus_tag="VMZ53_14135"
     CDS             1223..1624
                     /locus_tag="VMZ53_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29647.1"
                     /translation="MTTTIDRFESIHSTITITRPVPHVVVLTITGRDGGEHGNAPLRA
                     LDEQLKAGPYALFVDARETKGASVDVSNVWAQWLRAHRDDLHGIHMLTGSKFVQLTAD
                     FVRRFAELGDAMRVYTDGAAFDDELQTATRK"
     gene            1624..2106
                     /locus_tag="VMZ53_14140"
     CDS             1624..2106
                     /locus_tag="VMZ53_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29648.1"
                     /translation="MRIALLVVAVTASVAHAEPSASPPGLTSSIEDTEDVTEIQRIMG
                     PPVAFTLGFGLGHVVEGRWREKGWIFTTSETLATGLFVGGMLADMSCTQHCGYGGAAF
                     ATGMIALVGLRIWESVDAVNGARRKHARFMQRQMRLGLHLAPSKGGGDGAVAGLSLSF
                     "
     gene            complement(2103..2531)
                     /locus_tag="VMZ53_14145"
     CDS             complement(2103..2531)
                     /locus_tag="VMZ53_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29649.1"
                     /translation="MKSILVALVAITGCVANDPIEPEGASDDSTDSPTVDKIHQVDCM
                     PVMFVAGAGPYSVQCAALYSVSDAGVQVAYTATDSSGHEWVSARAAAPQSAGQASVVF
                     AIESASAPALGALTVHARLISDAQSTTASSEMSATIDVRY"
     gene            complement(2634..3350)
                     /locus_tag="VMZ53_14150"
     CDS             complement(2634..3350)
                     /locus_tag="VMZ53_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29650.1"
                     /translation="MASETKERERKERVPEETDTPYRGDREADHARIASLEGELAQAN
                     ARIAELEGKRSQALVLAGSKDLAATGKAKTASAKWFGAPLKLELTQTWDTEFPLDKLE
                     DLLDVIREATGDSGRVELLKTSMTWWSSSPEKGTGPFIEVRISVRDGKTRLEVKDRLG
                     QLAGVIYGAIGGGVGGGAIMLPIGASIAVPILAPVFALAWLGGGYFGLRKLFKSRARK
                     RAESLQRVFERVSAEIAKKS"
     gene            3388..5382
                     /locus_tag="VMZ53_14155"
     CDS             3388..5382
                     /locus_tag="VMZ53_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS29651.1"
                     /translation="MSLAVLDGVTLFFGDRMIFDNVSLRLGQGDRLGLIGPNGSGKTT
                     LLKVIAGEQEIDDGSVTRANGVRVGWLPQDIAIEGGRSLIDMILSSVPGRKELDERLA
                     RVEVDIEEATKQGRPEEELLELASEVGELHERIDHFDRFFGEHEAHAILAGLGFQTRD
                     ANRDLNEFSGGWKMRGVLAGLLFQRPDVLLLDEPTNHLDMPSVAWFGDFLQKWNRCFI
                     LISHDRDFLNDQINRVVSLELEGIRTYPGNYEKYVAARAEEETILAGKAKNLARERER
                     LTRFVDRFRAQANKAKAVQSRIKMLEKMEDVETYQKRGTMRFSFPDSARTVADVVRIE
                     KLKKAYGQHVVFPGIDLTVRRGEKIGIIGVNGAGKTTLLRMIAGELPHDSGTIKIGSG
                     VQVGYYAQHHADTLDMGATVYEIIQRAAPETPPARVRSMAGAFMFSGDDIDKPAKVLS
                     GGERARVALAKLCIAPGNLLLMDEPTNHLDLASSESLAQSLKTYDGTLVFVSHNRSLI
                     RQLATRIWNVEDGTVETYPGTLDEYMYSMAQRRQALAEAEDATTKKPAPQAAAPKISK
                     EEEKARKRREAEQRQKRSATLGPLEKQVAQLEERIATLEAEQKVRSSELADPTVYDDA
                     ARRNKLLADYQSSADKLEELTARWEKAMAQLEAAKAQLED"
     gene            complement(5379..>5764)
                     /locus_tag="VMZ53_14160"
     CDS             complement(5379..>5764)
                     /locus_tag="VMZ53_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29652.1"
                     /translation="RHDQFYGAIRRALDIEGLYEEAKEEITEIHEHAGARQAERINHV
                     LAFLTIVLTPMGMVVGVFQDKTLPESRRDDAFTLATFLTPSGWKDLLHHGPFQLLLLF
                     AVVGVALFLFFRTDRRIRDLGRTKR"
BASE COUNT         1008 a         1923 c         1891 g          942 t
ORIGIN      
        1 cgcacgccga cgagccgcga gtcgcagcgg atgtcgagcc gctggcggtc gagcgagtcg
       61 tgcctggcga ggcgtgcgac gacgcggtca tcgatccggt ggtgacgccg atgcgcgacg
      121 cctacctcga tgcgcagcgc agtgcgtgtc tgcgcgacga gctgtccgcg ggcctcgatg
      181 tgcacgcact gatcgacacc cctggctttc acggcgtgct cggcggcgac ctcgcgctcg
      241 gcggccgcat gatcgtcaag caggcgcacg agctgtccgt gcacgtgcgc gtcgtcgatt
      301 acgcgtttgt gcagaacgcg gtgaacaagg tgacgaacac cggcctcggg cccgtcgtcc
      361 tcggtgccgc ggcaggcaag gccatgtccg agaccgcgcg cgcggcactc gtacttcgcg
      421 tcgagctgcc gttcacgcgc gacaacatgg acacgatgcg cagcgccgcc gaaatcggtg
      481 gcgtcatgac ggggaagctc tcgagtagcg tcacgctgaa cgcacgcctg tccgcgctcg
      541 gcatgcacgc gtcctcgctc gccggctcca cctcgaggct cgcgtttcgc gccggtaccg
      601 atgtcgtctg gcaaccgcgc cgcacgatcg gcctgcaggc aggtgcggag ctgcaggccg
      661 gttggacccg cggcttcgat gcagtgctac tccgcgctgg gattcattgg gcgatccacg
      721 gcggaccgtg gcgattccgc tcgggcatcg gcgcgcccat cggcggcacc gaacgcacga
      781 atgcgatcgt cgacatcgcc gtccttcacg atctttgaga cccccaccgc tagcccgctc
      841 ctcgatcttc gacgtgctac gctcgcgccg tgccccgtgc ggacgatgcc aagactcgct
      901 tgaccgcgct ggctgcgcgg gtcgagagcg atccggccgc gctcgcggat cgcgcgaaag
      961 gcgatgtgct cgcgcacgaa gtcggcggcg aagccgcgct tcgctggcgc atcgcggtca
     1021 ttcgcgcgct gatcgcgaat cctcccgata gcgacgccgt ccgcgaagca tacggcgagc
     1081 ttgtcgaccg ctatcgcgac gacgcgaagg ccctgtcctc gatcaaatcg atcggcgacg
     1141 agattcgccg ccgagagtct gaaggctcgc tggcgagcac gatggttgct cggacgccac
     1201 gccccagaaa gcagaccaag tcatgacgac gaccatcgac cgcttcgaga gcatccattc
     1261 gaccatcacg atcacgcgcc cggtgccgca tgtcgtcgtc ttgacgatta caggccgcga
     1321 cggaggcgag cacggcaacg cgccgcttcg cgcgctcgac gagcaactga aggccgggcc
     1381 ctacgcgctg ttcgtcgatg cgcgcgagac caagggcgca tcggtcgatg tctcgaacgt
     1441 gtgggcgcag tggctgcgcg cgcatcgcga cgacctccat ggcatccaca tgctcaccgg
     1501 cagcaagttc gtgcagctca cggccgactt cgtgcgccgg tttgccgagc tcggcgatgc
     1561 gatgcgcgtc tacaccgacg gcgcggcgtt cgacgacgag ctgcagacgg cgacacgcaa
     1621 atgatgcgga tcgcgctgct tgttgttgcg gtgacggcga gcgtcgccca tgcggagccg
     1681 agtgcgtcgc cgccggggct gacttcgtcg atcgaagaca ccgaggacgt caccgagatc
     1741 cagcggatca tggggccgcc cgtcgcgttc acgctcggct ttggtctcgg ccacgttgtc
     1801 gaagggcggt ggcgcgagaa gggctggatc ttcacgacga gcgagacgtt ggcgaccggg
     1861 ctgttcgtcg gcggcatgct ggccgacatg agctgcacgc agcactgcgg ctacggcggc
     1921 gcggccttcg ccacgggcat gatcgcgctc gtcggcctgc gcatttggga gagcgtcgac
     1981 gcggtcaacg gtgcacggcg caagcacgcg cgcttcatgc agcgccagat gcgcctcgga
     2041 ctgcacctcg cgccatccaa aggcggcggc gacggcgcgg tcgctggact ctcgctgagc
     2101 ttttagtagc gcacgtcgat cgtcgcgctc atctcgctcg aagcggtcgt gctctgagcg
     2161 tcagagatga gccgcgcgtg cacggtcagc gcgccgaggg caggtgcgct cgcgctctcg
     2221 atcgcgaaca cgaccgacgc ttgtcccgct gattgcggtg cggcggcgcg tgcgctgacc
     2281 cactcgtggc cgctcgaatc ggtggcggtg tacgcgacct gcacgcctgc gtctgagacc
     2341 gagtagagag ccgcgcactg gaccgagtag ggacccgcgc ccgcgacgaa catgactggc
     2401 atgcagtcga cttgatggat cttgtcgacg gtggggctgt ccgtcgaatc gtcactggca
     2461 ccttctggct cgatcggatc gttggctacg catcctgtta tcgccacgag agccacgagg
     2521 atcgatttca tggcggcctg cttcgcaagg cgtgtgccgc cgtaggtgcc ccaaatgacg
     2581 tgcgcgaacg ctgttctgct gggggtgatg ccccggcgct ggtgcagcgt ccctcagctc
     2641 ttcttcgcga tttccgcgga gacgcgctcg aacacgcgtt ggagcgactc ggcacgcttg
     2701 cgtgcgcggc tcttgaacag cttgcgaagc ccgaagtagc cgccaccgag ccacgcgagc
     2761 gcaaacacgg gcgcgaggat cgggaccgcg atcgacgcac cgatcggcag catgatcgcg
     2821 ccaccgccaa cacctccgcc gatcgcgccg tagatgacgc cggcgagctg accgaggcgg
     2881 tctttcactt cgaggcgcgt cttgccgtcg cgcaccgaga tgcgaacctc gatgaagggt
     2941 cccgtgcctt tttctggcga cgacgaccac cacgtcatcg acgtcttcag cagctcgacg
     3001 cggccgctat cgccggtggc ctcgcgaatg acgtcgagca aatcttcgag cttgtcgagg
     3061 ggaaattcgg tgtcccacgt ctgcgtcagc tcgagcttga gcggcgcgcc gaaccacttc
     3121 gccgacgcgg tcttcgcttt gcccgtcgca gcgaggtcct tgctccccgc gagcacgagc
     3181 gcttgcgagc gctttccttc gagctcggcg attcgcgcgt tcgcctgcgc gagctcaccc
     3241 tcgagcgacg cgatccgcgc gtggtcggct tcccggtcgc cacggtacgg cgtgtccgtc
     3301 tcctccggca cgcgctcttt gcgctcacgt tccttcgtct cgctcgccat tccgcggcga
     3361 gtgtaacgtg ctatttagtc gccgctcatg agtctcgcgg ttctcgacgg cgtcacgttg
     3421 ttcttcggag atcgaatgat cttcgacaac gtgagcttgc gcctcggcca gggtgaccgc
     3481 ctcggcctca tcgggccaaa cggcagcggc aagacgacgc tgctgaaggt cattgccggc
     3541 gagcaagaga tcgacgatgg cagcgtgact cgcgcgaatg gtgtgcgcgt cggctggttg
     3601 ccacaggaca tcgcgatcga aggcggtcgc tcgctcatcg acatgatctt gtcgagcgtg
     3661 cccgggcgca aggagctcga cgagcggctc gcccgcgtcg aagtcgacat cgaagaagcg
     3721 acgaagcagg gcaggcccga ggaagagctg ctcgagctcg catcggaagt cggcgagctc
     3781 cacgagcgca tcgatcactt cgatcgcttc ttcggcgagc acgaagcgca cgcgatcctc
     3841 gcgggcctcg ggtttcagac gcgcgatgcc aaccgcgacc tcaacgaatt ctccggcggc
     3901 tggaagatgc gcggcgtgct cgcggggctg ttgttccagc gcccggacgt cctcctgctc
     3961 gacgagccga ccaaccatct cgacatgccc tcggtcgctt ggttcggcga ttttctccag
     4021 aagtggaatc gctgcttcat cctcatctcg cacgatcgcg acttcttgaa cgatcagatc
     4081 aatcgcgtcg tcagcctcga gctcgaaggc attcggacgt atcccggcaa ctacgagaag
     4141 tacgtcgcgg cgcgcgccga agaggagacg atcctcgccg gcaaagcgaa gaacctcgct
     4201 cgcgagcgag agagactcac gcgcttcgtc gatcgcttcc gcgcgcaggc gaacaaggcg
     4261 aaagccgtgc agtcgcgcat caagatgctc gagaagatgg aggacgtcga gacgtatcag
     4321 aagcgcggca cgatgcgctt ctcgttcccc gactccgcac gcaccgtcgc agatgtcgtg
     4381 cgcatcgaga agctgaagaa ggcgtacggc cagcacgtcg tgttcccggg catcgatctc
     4441 accgttcgtc gcggcgagaa gatcggcatc atcggcgtca acggcgcggg caagaccacg
     4501 ctcctgcgca tgatcgcggg agagcttccg cacgactccg gcacgatcaa gatcggctcg
     4561 ggcgtgcaag tcggctacta cgcgcagcac catgccgaca cgctcgacat gggcgcgacc
     4621 gtctacgaga tcatccagcg cgctgcgccc gagacgccac cggcacgcgt gcgctcgatg
     4681 gcaggcgcct tcatgttctc gggcgacgac atcgacaaac ccgccaaggt tctctcgggt
     4741 ggtgagcgcg cgcgcgtcgc gctggcgaag ctctgcatcg cgccgggcaa cctcctcctg
     4801 atggacgagc cgacgaacca cctcgacctc gcatcgagcg agagtctcgc gcagtcgctc
     4861 aagacctacg acggcacgct cgtgttcgtc agccacaacc gctcgctcat ccgccagctc
     4921 gcgacgcgga tctggaacgt cgaggatggc accgtcgaga cctacccggg cacgctcgac
     4981 gagtacatgt actcgatggc gcagcggcgc caagcgctcg ccgaagcgga agacgcaacg
     5041 acgaagaagc ccgctccgca ggccgccgcc ccgaagatca gcaaagagga agaaaaggcg
     5101 cgcaagcggc gtgaagccga gcagcggcaa aaacgctccg cgacgctcgg accgctcgag
     5161 aagcaggtcg cgcagctcga ggagcgcatc gcgacgctcg aagccgagca aaaagttcgc
     5221 tcgagcgagc tcgccgaccc gaccgtctac gacgacgccg cgcgccgcaa caagctcctc
     5281 gccgactatc aatcgtcggc cgacaagctc gaagagctca ccgcgcggtg ggaaaaggcg
     5341 atggcgcagc tcgaagccgc gaaggcgcag ctcgaggact aacgcttcgt cctgccgagg
     5401 tcgcgaatcc ggcggtcggt gcggaaaaac aagaacagcg cgacgccgac gaccgcgaac
     5461 agaagcagca gctggaacgg gccgtggtgg aggagatcct tccagccact cggcgtcagg
     5521 aacgtagcga gcgtgaacgc gtcgtcacgg cgtgactcgg gaagtgtctt gtcctggaaa
     5581 actccgacca ccatgcccat cggagtgagc acgatcgtga gaaacgcgag cacgtgattg
     5641 atgcgctccg cttggcgcgc gccggcgtgc tcgtggatct cggtaatctc ttccttggcc
     5701 tcttcgtaca ggccctcgat gtcgagcgcg cggcggatcg caccgtagaa ctgatcgtgg
     5761 cgct
//
LOCUS       DATLBZ010000270         8342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9840359, whole genome shotgun sequence.
ACCESSION   DATLBZ010000270 DATLBZ010000000
VERSION     DATLBZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9840359"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VMZ53_14165"
     CDS             complement(<1..198)
                     /locus_tag="VMZ53_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29653.1"
                     /translation="MRHLTSKRRFRFTALAGLAVVVALAGVAVATDSFALISNYSQVI
                     KFVGLNGDGTADSPISINTATQ"
     gene            complement(338..856)
                     /locus_tag="VMZ53_14170"
     CDS             complement(338..856)
                     /locus_tag="VMZ53_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29654.1"
                     /translation="MRGFFALMLASAALLSAACAPTIDGPLEHQRAVDRADAAHLASQ
                     LAQLPGAVRAEVTLHRPVVDPLTEATTPASAAILVVVDDKADRRAITRSAIALARGTA
                     PEIAEPEIVVELGATRPTLASVGPFSVEARSKPTLVGVLAAVLGLVAALAAWIAWRER
                     WRLSGGSQPSSR"
     gene            944..1933
                     /locus_tag="VMZ53_14175"
     CDS             944..1933
                     /locus_tag="VMZ53_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29655.1"
                     /translation="MNAVRTYARLLPVEAARRLAALRVMRFDAAVGIGGVMAPLGRPI
                     VLDADEARERVLDRWEGDLCGLLNAMTRGELEWLAAMVDVKREGRAPELRERLWGHGS
                     LLERCGEDVPASVQPRPVLLGGHLVVMAPPRGMFPQSESWPRPVPAERVAQPPSEEPD
                     SLDELLAAADRAIGVRLGRRGRDKGAWGMRAARLLGVEERGGGEPDWRGDVEIKTVPV
                     AREASGLWRIVEDPAIAMIGEGGALAKLQRTLWLARADVDGPDGDADATIVCWYLLDW
                     DPMVARLVRRYLHVRPKGPKGTDQRGLYLHKRFFAECGLLAALNGPAPGPRPR"
     gene            1930..3120
                     /locus_tag="VMZ53_14180"
     CDS             1930..3120
                     /locus_tag="VMZ53_14180"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29656.1"
                     /translation="MIIDELRIVGSTQTNVVMSAELIRVAQRAVKVRPPNPRKEGLGQ
                     LVYPFDRELAWAAVTYMRTPTRVLWDVYRSQSERLEPLYDELCADIANDGRALWRDGD
                     GISVEARHVENFPAGERQIVGTVKNALVDTMARRGIRLRVDPQRPATRWVARQDDKGE
                     MVISIDLGGGSLSQRGWRRDAGEAPLREHLAAVLLMLCRFDPRRDALVDPMCGSGTIP
                     IEAVLSARATPRPTPGLSALGIKYVAEDAKSLEPAPLYSDAAPLVVGCDIDLDVLAAA
                     RDNARAARVGEAITWQRADVATLTPAIIDDIARERGREPPREGVLLSNPPYGERLDEA
                     DLVPIYRALAETVQRFPRGWRAGFLVGNPLLEEVFADAGGRPRIKKPLANANLRAYFY
                     LYEL"
     gene            complement(3117..3629)
                     /locus_tag="VMZ53_14185"
     CDS             complement(3117..3629)
                     /locus_tag="VMZ53_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29657.1"
                     /translation="MDPVDRRNLHITGRHKAVTGSHVAVQPAPSPATDDVVVIVTQAY
                     GPQGHNLVGIADVQFDGHPAVTVGLRAEGREGLVHLSPIHGDGRKQGLTDLPQGTRCE
                     LYCPVSGQSLDLVGEVEDGSGARYYAIYLTPQLSRGSMVMVSDVWGHYHSRIIDDFEL
                     ISAWAAQQED"
     gene            3694..5142
                     /locus_tag="VMZ53_14190"
     CDS             3694..5142
                     /locus_tag="VMZ53_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29658.1"
                     /translation="MRSLVLAIGVVLLLSSAAWAKPRVALAPFEGDSGGAVSDMVAEL
                     LEGDYTVSGPSQVQRKIDELGYGDNLSDKQLKKLANELEAEAIVRGDLATKGKHKVLH
                     VRLFVKGKRVKGFKVEFGSTRSQKVKDALKEKLLEKLGGDSGGGGGGEDEKPAKVEET
                     DTPAETDEKPEKPEKPEKKKPVEQETDEEGDEPPGGKKKTATAEEDEEEIDETVVPLT
                     KKSELHSANRAAVRVDFGPSASSRQLLFTSRNFEQAPKPYKNAVVPGGRVAGELYPLA
                     FGNPNGIVAGLGLGGHFDQTIGLKLRSTAQLGTQFPVDQKHWSVGARFRVAFGTKPTS
                     PTVTLRGGIFHRKFTVDRTALEMGNVIDLPDVLYQGYDPGVDIRFPIIRPIALVLGGE
                     AYLVTNTGSIQKLNQYGQARVTGGEGYAGLDISIGSRLAVRALVEASQVGFAFTGNGE
                     LSNNRDGDPSTPDVGGAADRVIGGSLTVGVLY"
     gene            5187..8072
                     /gene="gcvP"
                     /locus_tag="VMZ53_14195"
     CDS             5187..8072
                     /gene="gcvP"
                     /locus_tag="VMZ53_14195"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HUS29659.1"
                     /translation="MQPDVLAPSDTFARRHIGPQKSETAEMLALLGYKSLDELADATV
                     PQKIRLKSPLRLGPALGEHELLEAMKNLASDNLVMRSFIGQGYYDTITPPVILRNVLE
                     NPGWYTQYTPYQAEISQGRLEALINFQQMIEDLTGLPMANASLLDEATAAAEAMHMCF
                     GQAEHKRAAFWVQDATHPQTIAVMRTRAEPLGIDLRVGSVQQIVAALDDTVAGVLLSY
                     PTTDGQVVDLRDVIAKIHAAGAGVAMATDLLALTLLTPPGELGADIAIGTAQRFGVPM
                     GFGGPHAAFLSSKDEYRRQMPGRIIGVSRDAKGKTAFRLALQTREQHIRREKATSNIC
                     TAQVLLAVMASMYAVYHGPEGLTRIAQRVRAYTSLVAAGLGKLGVKVRGGAFFDTLRV
                     DLDPHAQARVLARALERGINLRRYADGLGISCDETTVSADVEALLEAFATPNADGDAL
                     PFTIEDLVKSTNVPELPKGLARTSQFLTHPTFHRYQAEHEMLRYLNRLTTKDLSLTTS
                     MIPLGSCTMKLNATTEMLPVTWPEFGRMHPFAPAEQWGGYKAMFEQLEAWLAEITGFA
                     AVSLQPNSGAQGEYAGLLAIRGYHRARNEAHRTVCLIPQSAHGTNPASAVMAGMEVVV
                     VGTAPDGTIDLADLEAKAKKHAAKLSCVMVTYPSTHGVFEDGITELCAIVHANGGQVY
                     MDGANMNAQVGLTRPAECGADVCHLNLHKTFCIPHGGGGPGMGPIAVAQHLAPYLPGH
                     PLAPAGSQPVGPVSAAPWGSASILPISFAYIAMMGEAGLRRATEVAILGANYIAKRLG
                     EHYPVLYTGQNGRVAHELILDCRGFKKTAGIEAEDIAKRLMDYGFHAPTMSFPVPGTL
                     MVEPTESEAKSELDRFCDAMIAIRAEIAAIENGQMDRANNPLKNAPHTANAVMSTEWD
                     RPYTREQAAYPAPWLRAHKYWPPVARVDNAYGDRNLVCACPPVDAYS"
     gene            8100..>8342
                     /locus_tag="VMZ53_14200"
     CDS             8100..>8342
                     /locus_tag="VMZ53_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29660.1"
                     /translation="MDLVGLIAVLGAFAVPLYALKTRHEAKMRELQAKNPQLQLQAGK
                     QAQLVLEENKMLRERVENLEAIVCSVDYELNKKLAKV"
BASE COUNT         1439 a         2659 c         2916 g         1328 t
ORIGIN      
        1 ttgcgtcgcg gtattgatgc tgatgggcga gtcagccgtc ccgtcgccgt tcaggccgac
       61 gaacttgatg acttgggagt agttactgat cagcgcgaag ctgtcggtcg cgactgcgac
      121 gccagccaac gcgaccacca ccgcgagtcc agctaaggca gtgaaacgaa aacgacgctt
      181 actggtgaga tgccgcatgt ttgatcacac ccttctacat cagtcgcgat cgcggcctgg
      241 gttacgcgcg gggtaacgag atcgtttgag tgtcaactat cgtgttcatc gcgacgcgat
      301 cgagcggctg tgatcgaagg tgcccgcggc gaacgcgtca acgtgaagac ggttgtgagc
      361 cgcccgacag gcgccagcgt tcccgccaag cgatccacgc cgcgagcgca gcgacgagac
      421 caagcacggc ggccagcaca ccgacgagcg ttggcttcga gcgcgcctcg acgctgaacg
      481 ggccgacgga ggcgagcgtg gggcgcgttg cgccgagctc gacgacgatc tcgggctccg
      541 cgatttcggg cgcggtgccg cgcgcgagcg cgatagctga gcgcgtgatc gcgcggcggt
      601 cggctttgtc gtcgacgacg acgaggatcg cggcggatgc gggtgtcgtg gcttctgtga
      661 gcggatcgac gacggggcgg tgaagcgtga cttcggcgcg gaccgcgccg gggagttgcg
      721 cgagctgcga ggcgaggtga gccgcgtcgg cgcgatcgac ggcgcgttgg tgctcgaggg
      781 gaccgtcgat cgtcggagcg caggctgcgg acagcagcgc ggcggacgcg agcatcagtg
      841 cgaagaatcc gcgcacggga gcacgctaac acgcggaggg agatcgctct ttggtcgaga
      901 cgcgtgcgcg cgctcccggt ggaagcggtt taacgtggtg agcgtgaatg ccgtgcggac
      961 gtatgcgcgc ctgctgcccg tcgaagcggc gcgccggctc gcggcattgc gcgtgatgcg
     1021 gtttgatgcg gcggtgggca ttggaggcgt gatggcaccg ctggggcggc cgatcgtgct
     1081 cgatgccgac gaggcccgcg agcgcgttct ggatcggtgg gaaggcgatc tgtgcgggct
     1141 gctcaatgcg atgacacgcg gcgagctcga gtggttggcc gcgatggtcg atgtgaagcg
     1201 tgagggcagg gcgccggaat tgcgcgagcg gctgtggggg catggctcgc tgctcgagcg
     1261 gtgcggcgag gatgtgccgg cgagcgtgca gccgaggccc gtgctgctcg gtgggcatct
     1321 cgtcgtgatg gcgcctccgc gcggcatgtt tccgcagagc gagagctggc cgcgtcccgt
     1381 gcccgccgag cgagtcgcgc agccgccgag cgaggagccc gacagcctcg atgaattgct
     1441 ggctgcggcg gatcgcgcga tcggcgtgcg actcgggcgg cgaggacgcg acaagggcgc
     1501 gtggggcatg cgggcggcgc ggttgctagg cgtcgaggag aggggtggcg gagagcccga
     1561 ttggcgcggc gacgtcgaga tcaagaccgt gcccgtcgcg cgcgaagcga gcgggctgtg
     1621 gcgcatcgtc gaggatccgg cgatcgcgat gatcggcgag ggtggcgcgc tcgcgaagct
     1681 gcagcggacg ttgtggctcg cacgcgcgga tgtcgacggg cccgacggcg atgccgacgc
     1741 gaccatcgtc tgttggtatc tgctcgattg ggatccgatg gtcgcgcgac tcgtgcggcg
     1801 atatctgcac gtgcggccga agggcccgaa gggcacggac cagcgcgggc tgtatttgca
     1861 caagcgattc ttcgccgagt gcgggctgct cgctgcgttg aatggacccg caccgggacc
     1921 gcggccgcga tgatcatcga cgagcttcgc attgtcggct cgacgcagac gaacgtcgtc
     1981 atgagcgcag agctcattcg cgtcgcgcag cgcgcggtga aagtgcggcc gcccaatccc
     2041 cgcaaggaag gcttggggca gctcgtctat ccgttcgatc gcgagctcgc gtgggcggca
     2101 gtgacgtaca tgcgcacgcc gacgcgcgtg ctctgggatg tgtatcgctc gcaatcggag
     2161 cggctcgagc cgctctacga cgagctgtgc gccgacatcg cgaacgacgg gcgcgcgctg
     2221 tggcgcgacg gcgatgggat ctctgtcgag gccaggcacg tcgagaactt tcctgctggc
     2281 gagcgacaga tcgtcggcac ggtgaagaac gcgctcgtcg acacgatggc gcggcgtggg
     2341 attcggctcc gcgtcgatcc gcagaggccc gcgacgcgat gggtggcgcg gcaagatgac
     2401 aagggcgaga tggtcatctc gatcgatttg ggcggcggct cgctctcgca gcgaggctgg
     2461 cgacgtgacg ccggtgaggc gccgcttcgc gagcatctag cggcggtgtt gctgatgctg
     2521 tgtcgcttcg atccgcgccg cgacgcactc gtcgatccga tgtgtggctc tggaacgatt
     2581 ccgatcgaag ccgtcctgtc cgcgcgcgcg acgccgcgcc cgacgccggg cctgtctgcg
     2641 ctcgggatca agtacgtggc cgaggacgcg aagagcctcg agcccgcgcc gctctattcg
     2701 gacgccgcgc cgctcgtcgt cggctgcgat atcgatctgg atgtcctcgc agcggcgcgt
     2761 gacaacgcga gggcggcgcg cgtcggtgaa gcgatcacgt ggcagcgcgc cgacgttgcg
     2821 acgctcacgc ccgcgatcat cgacgacatt gcgcgcgagc gtggccgcga gccgccgcgt
     2881 gaaggcgtgt tgctgtcgaa tcctccgtat ggcgaacgcc tcgacgaagc tgacctcgtg
     2941 ccgatctatc gcgcactcgc agaaacagtt cagcggtttc cgcgcggctg gcgcgccgga
     3001 tttctcgtcg gcaatccgct cctcgaagaa gtgtttgccg atgcgggcgg caggccgcgg
     3061 atcaagaagc cgctcgcgaa tgcgaacctg cgcgcgtact tctatttgta cgagctctag
     3121 tcctcttgct gcgcagccca ggcgctgatc agctcgaagt catcgatgat gcgcgagtgg
     3181 tagtgccccc acacgtcgct gaccatcacc atcgatccgc gcgacagctg cggcgtgagg
     3241 tagatggcgt agtaacgcgc gcccgagccg tcttctacct cgccgaccag atcgagcgat
     3301 tgccctgaca cggggcagta cagctcgcaa cgtgttcctt gcggcagatc cgtcagacct
     3361 tgtttgcgac catcgccgtg aatcggggac aggtggacga ggccttcgcg accttccgcc
     3421 cgcaggccga ccgtcaccgc ggggtggcca tcgaactgca cgtccgcgat tccgacgagg
     3481 ttgtggccct gtggcccgta cgcctgcgtg acgatcacca cgacgtcatc cgtcgccggc
     3541 gaaggggctg gctgcactgc gacatgcgag ccggtgacgg ccttgtgtct gccggtgata
     3601 tggaggtttc ggcgatcgac ggggtccacg aagcgagctg acaacgcctg caaggggatc
     3661 gcaagcgctc accgtgtata ctcgtggctc gcgatgagaa gccttgtcct cgcgatcggc
     3721 gtggtgctgc tgcttagctc agcagcctgg gccaagccac gcgtggcgct cgcccccttc
     3781 gagggcgata gcggcggtgc tgtctcggac atggtcgcgg agctgctgga gggcgactac
     3841 acggtcagcg ggccgtcgca ggtccagcgc aagatcgacg agctgggcta cggcgacaac
     3901 ctgagcgaca agcagctcaa gaagctcgcc aacgagctcg aggccgaagc gatcgtgcgc
     3961 ggcgatttgg cgacgaaggg caagcacaaa gtcctgcacg tgcggttgtt cgtgaagggc
     4021 aagcgcgtga agggcttcaa ggtcgagttc ggcagtacgc gctcgcaaaa ggtgaaggac
     4081 gcgctcaagg aaaagctcct cgagaagctc ggcggcgaca gcggcggcgg cggcggcgga
     4141 gaagacgaga agccggcgaa ggtcgaggag accgacacgc ccgccgagac ggacgagaag
     4201 cccgagaagc ccgagaagcc cgagaagaag aagcccgtcg agcaagagac cgacgaagaa
     4261 ggcgacgagc cgcccggcgg caagaagaag accgcgacgg ccgaagaaga cgaagaagag
     4321 atcgacgaga cggtcgtacc gctcacgaag aagagcgagc tccacagcgc gaatcgcgcc
     4381 gcggtgcgcg tcgacttcgg tccgtcggcg tcgagccggc agctgctgtt cacgtcgcgc
     4441 aacttcgagc aggcacccaa gccgtacaag aacgccgtcg ttcccggcgg gcgcgtcgcc
     4501 ggtgagctgt atccgctcgc attcggcaac cccaatggca tcgtcgcggg cctcgggctc
     4561 ggcggtcact tcgatcagac gatcggcctg aagctccgca gcaccgcgca gctcgggacg
     4621 cagtttcccg tcgaccagaa gcattggtct gtcggtgcgc ggttccgcgt cgcgttcggc
     4681 acgaagccga cgagcccgac ggtgacgctg cgcggcggca tctttcatcg caagttcacc
     4741 gtcgatcgca ccgcgctcga gatgggcaac gtgatcgacc tgccggatgt gctctatcaa
     4801 ggctacgacc cgggcgtcga tattcgattc ccgatcatcc gccccatcgc actcgtgctc
     4861 ggaggcgagg cgtatctcgt cacgaacacc ggctcgatcc agaagctgaa ccaatacggt
     4921 caggcgcgcg tgaccggcgg cgagggctac gcgggcctcg acatctcgat cggcagccgg
     4981 ctcgcggtgc gggctctcgt cgaagcatcg caggtcggtt tcgcgttcac gggcaacggt
     5041 gagctgtcga acaatcgcga tggcgatccg tcgacacctg acgttggcgg cgctgccgat
     5101 cgcgtgatcg gcggatcgct cacggtcggc gttctgtact gattcgtcga cgatcagcgg
     5161 ccgttcggac gtgctagggt gcgcccgtgc aacccgacgt tctcgcacca tcggatactt
     5221 tcgcacgacg tcacatcggg ccgcagaagt ccgagaccgc cgagatgctc gcgctgctcg
     5281 gctacaagtc gctcgacgag ctcgcggacg cgaccgttcc acagaagatc cggctcaagt
     5341 cgccgctgcg tctcggtccc gccctcggcg agcacgagct gctcgaagcg atgaagaacc
     5401 tcgcgtccga caacctcgtc atgcgctcgt tcatcggcca gggctactac gacacgatca
     5461 cgccgcccgt gatcctgcgc aacgtgctcg agaacccggg ctggtacacg cagtacacgc
     5521 cgtaccaggc tgagatttcg caaggccgcc tcgaggcgct gatcaacttc cagcagatga
     5581 tcgaggacct caccgggctg ccgatggcaa acgcatcgct gctcgacgaa gcgaccgccg
     5641 ctgctgaagc gatgcacatg tgcttcggcc aggcggagca caagcgcgcc gcgttctggg
     5701 tgcaggacgc aacgcatccg caaacgatcg ccgtcatgcg cacgcgcgcc gagccactcg
     5761 gcatcgatct gcgcgtcggc tccgtgcagc agatcgtcgc tgcgctcgac gacaccgtcg
     5821 cgggcgtgct gctgtcgtat ccgacgacgg acggtcaggt cgtcgacctc cgcgatgtga
     5881 tcgcgaagat tcacgccgcg ggtgccggcg tcgcaatggc gacggacctt ctcgcgctga
     5941 cgctgctcac gcctcctggc gagctcggcg cggatatcgc gatcggcacg gcgcagcgct
     6001 tcggcgtccc gatgggcttc ggtggcccgc acgccgcgtt cctgtcgtcg aaggacgagt
     6061 accgacggca gatgccgggc cgcatcattg gcgtctcgcg cgacgcgaag ggcaagaccg
     6121 cattccgcct cgcgctgcag acgcgcgagc aacacatccg ccgcgagaag gcgacgtcga
     6181 acatctgcac cgcgcaggtg ctcctggccg tgatggcctc gatgtacgcc gtctatcacg
     6241 gccccgaagg cctcacgagg atcgcgcagc gcgtccgcgc gtacacgtcg ctcgtcgcgg
     6301 ccggtctcgg caagctcggc gtgaaagttc gcggcggcgc gttcttcgac acgctgcgcg
     6361 tcgatctcga tcctcatgcg caggctcgcg tgctcgcacg agcgctcgag cgcggcatca
     6421 atctgcggcg ctacgccgac ggcctcggca tctcgtgcga cgagacgacg gtgtcggcag
     6481 acgtcgaagc gctgctcgag gcattcgcga caccaaacgc ggacggagat gcgctgccgt
     6541 tcacgatcga agatctcgtg aagtcgacga acgtgcccga gctgccaaag ggcctcgcgc
     6601 gaacgagcca gttcctcacg cacccgacgt tccatcgcta tcaggccgag cacgagatgt
     6661 tgcgttatct caaccggctc acgacgaagg acctgtcgct cacgacgtcg atgatcccgc
     6721 tcggctcgtg cacgatgaag ctcaacgcga cgacggagat gctccccgtc acgtggcccg
     6781 agttcggccg catgcatccg ttcgcgcctg ccgagcagtg gggcggctac aaggcgatgt
     6841 tcgagcagct cgaggcgtgg ctcgcggaga tcaccggctt cgcggccgtc tctctgcagc
     6901 cgaactctgg cgcgcaaggc gagtacgcag gcctgctcgc gattcgcggc taccaccgcg
     6961 cgcgcaacga agcgcatcgc acggtgtgcc tgatcccgca gagcgcgcac ggcacgaatc
     7021 ccgcgtcggc cgtgatggcc ggcatggaag tcgtcgtcgt cggcacggcg cctgacggca
     7081 cgatcgatct tgcagacctc gaggcgaaag cgaagaagca tgccgcgaag ctctcgtgcg
     7141 tgatggtcac gtatccgtcg acgcacggcg tgttcgagga cggcatcacc gagctctgcg
     7201 cgatcgttca cgcgaacggc ggccaggtct acatggacgg cgcgaacatg aacgcacagg
     7261 tcggcctgac gcggcctgct gaatgcggcg cagatgtctg tcacctgaat ctgcacaaga
     7321 cgttctgcat cccgcacggt ggcggtggtc ccggcatggg cccgatcgcc gtcgcgcagc
     7381 acctcgcgcc gtatctgccc ggtcacccgc tcgcacccgc gggcagccag ccggtcggtc
     7441 cggtgtcggc agcgccgtgg ggctccgcgt cgattctccc gatctcgttc gcgtacatcg
     7501 cgatgatggg cgaggcgggt ctgcgtcgcg cgacggaagt cgcgatcctc ggcgcgaact
     7561 acatcgcgaa gcggctcggc gagcactatc ccgtgctcta caccggccag aacggtcgcg
     7621 tcgcgcacga gctcatcctc gattgtcgcg gcttcaagaa gactgccggc atcgaggccg
     7681 aggacatcgc gaagcgcctc atggactacg gcttccacgc gccgacgatg tcgttcccgg
     7741 tgccgggcac gctgatggtc gagccgacgg agagcgaggc gaagtccgag ctcgatcggt
     7801 tctgcgacgc gatgatcgcg atccgcgcgg agattgcagc gatcgagaac ggccagatgg
     7861 atcgcgcgaa caacccgctc aagaacgcgc cacacacggc gaacgccgtg atgagcaccg
     7921 agtgggatcg accgtacacg cgcgagcaag cggcgtatcc ggcgccgtgg ctgcgcgcgc
     7981 ataagtactg gccgccggtc gcgcgcgtcg acaacgccta cggcgatcgc aatctcgtgt
     8041 gcgcgtgccc gcctgtcgac gcgtactcgt agcccggctt tgatgcatag tcggggtcca
     8101 tggacctcgt tggcctcatt gcggtgctcg gcgcgttcgc cgtgccgctc tatgcgctga
     8161 agacgcgaca cgaggccaag atgcgcgagc tgcaggcgaa gaacccgcag ctgcagctgc
     8221 aggccggcaa gcaagcgcaa ctcgtgctcg aagagaacaa gatgctgcgc gagcgcgtcg
     8281 agaatctcga agcgatcgtc tgctccgtcg actacgagct gaacaagaag ctcgcgaaag
     8341 tg
//
LOCUS       DATLBZ010000271         7392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9238324, whole genome shotgun sequence.
ACCESSION   DATLBZ010000271 DATLBZ010000000
VERSION     DATLBZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9238324"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VMZ53_14205"
     CDS             complement(<1..1200)
                     /locus_tag="VMZ53_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29661.1"
                     /translation="MALALVALGGLFGCQIGCDGERAHKSQTPHPPVAVEDSGLGALP
                     ASASSPQLTPEERGALDVSPGSLDYLFTALAAAEAGEPGGRVDVIVFGDSHTAGDAMT
                     GRLRLVWQRRFGDAGRGVVAAGKPPVKHYYQRDVKYGRTGTWTGAVGGKRGDSEPFGI
                     AGLRVSGTKKGAQLWVETCADCPSGKTTAQFEILYYAAPDHGLLRYKVDDGKWLDLST
                     KTTAIEPPHPARQVVPVAEGAHKLTLEHGGGGPVDLFGVVMENVKPGVIIDALGVVGR
                     RLGSLRSWDWSIIGEQLATRDPRLVILQYGTNEADDPELVLEDMARYYDETILRIRAA
                     APTASILILGPPDMGVREAGKSCDKMKPPKGGVDAGVIPECEWHTPGILREIVAVEHA
                     AAVRNKVA"
     gene            complement(1202..2587)
                     /locus_tag="VMZ53_14210"
     CDS             complement(1202..2587)
                     /locus_tag="VMZ53_14210"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HUS29662.1"
                     /translation="MSAQDRGWSVAPTLVTFVGVFAASLFLLDGNFSALADRLTTKRE
                     PEAAGAGPVIVAKSDQASAVEEADLVAALPTPPAGSHDIGALDDVCIDGTEEKCAKWA
                     MDGFYRAVSEAKNAKLGRALRVSWYGDSVSASDELPGRLRSRLQAELGDGGPGFVYIL
                     PPHRFCHHDAITRSGGDNFLSYAISTNHVTDGFYGPGGATVETNSGRSTIKLVNGKVT
                     SAELYYLAQPKGGTTTIAADGTDIASISTLADAKAPGFLVGSIAAGATKFEIRGEGKT
                     RLFGVSLENATGAVVDNFGIVSVHTKSFAASQSEHFKTELEHRSADLIIIMIGANEAM
                     WLKAGDSTQKTYQAEYEKMLVPFRKARPDASCLVVSPSDQAEAKDDGYQSKPVMRTIA
                     DAQRKAAHASGCAFYSTYDWMGGKGSAMKWSKKHLIGGDFIHLTHAGASKMADGLFDS
                     LLAGSKSYATK"
     gene            2688..4016
                     /locus_tag="VMZ53_14215"
     CDS             2688..4016
                     /locus_tag="VMZ53_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29663.1"
                     /translation="MQKSLRIGFALCVLVGCGDDAATTPDSPTGNRPPPRAISGGGIG
                     DGAIDGVVNLYVMDDATRMPLANATVRVGTLDGTTDATGLFVAEGVVGPQTVLAKLAG
                     YRSEMWIGANGANMTVSLKPDVDPTPARAKLSGSITGFDQLTVPTGHRKAAIVTYSND
                     DKAGDAANNIDTANATNICDAGTGMPCNFSITTRTGTVALIAIVYDHDTKNTLTPNDD
                     TYTPITWAYKTGISVAGGVDQTGIALSLVDAADLANVIVDFGTPPAGMANIAAVIGIE
                     VGSSGTMQLVPSFATQSAPTVLAPKLSAISGATYRLTAIAQNGNTELDPGSFVLHRAQ
                     TSTSLGAGTWLGVPSGVTLTRTTASWTAVPGAALQGVEYDIDKTHHVLSITSFDGSTS
                     ATIPELLALPSSPLVGRVTALQGTVDLMDFSVDDVLTKITGFSAQPLQIN"
     gene            4044..5012
                     /locus_tag="VMZ53_14220"
     CDS             4044..5012
                     /locus_tag="VMZ53_14220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUS29664.1"
                     /translation="MRWLLVVTAALGCGPTASQSPQPSYFTMNGPSPLLAPAAASYDP
                     TQAGTVVDRMVDFAFDGGDIEFEMRRDGGRVIEVARNHYMVPVVMQWTVGDLDNLLPS
                     GAVSGVVVLPPADRPNGTGAGVLLTTYNLDDPRAAYRRQLNMHARFGDPNARPQPYAY
                     RLPFKAGKTFSILQGFHGTFSHRGSNEFAVDFDCPVATPVLATRPGIVVAANAAAQGS
                     GTTPDFLEYRRTNFVLVLHDDGTLGEYMHLAASGVRASVGQKVERGDELALSGNTGYS
                     STPHLHFQVMTAAGDGISAQSFPFELQISAHKVEPPVQGRSYPSWE"
     gene            5052..6437
                     /locus_tag="VMZ53_14225"
     CDS             5052..6437
                     /locus_tag="VMZ53_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29665.1"
                     /translation="MGRAWFIAVGLTLASSAAAAPLEAPQFTIRPQGAMAAFTGETVS
                     ITIGWRPVAQAAKYRLAITDAKNQTTQIDVTGLRHELTDLAPGKYQVTVAALDASGMA
                     GPTSELLYLSVIEMRAIPPGSSQSMPPQRGAYAVGTRFAVGGMHCEFSDAPIDDLLVG
                     PENEVRMPIAGVARLRCAGIPGYLEKQVVIAPVAIQTVTPATRGVTTDVHVTVASVAF
                     LGEQLDVEAIGDLTLGDAQRTDFGFEVPVHVAPTATSAALSIQTGDFELGRTSLQLID
                     APPPPPPPVRKPLDWRALDLGGHAGAFVPSDGGHAPTIGMPTDPADVVSGGPLVGLRL
                     GFFPTRRVGLEGEISLIEAGHGDREGTSHVVAGRAQLAVRAVESGNLGLRVIAGTGTF
                     TNLSESGTARVATQGEIHAGGALTIETSPNLWLRFQFVDAVTTARDDGYSHCLELQLG
                     VVTRVGRRDSW"
     gene            6448..7338
                     /locus_tag="VMZ53_14230"
     CDS             6448..7338
                     /locus_tag="VMZ53_14230"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS29666.1"
                     /translation="MRCPVCSERPAEVGVLCEECRDELDSPIKISQEQILARVQTPTN
                     AALIDLWGRPHQLDAHARIGRQIEAGGVAVLEPSVSRLHAELVFEGISWTIRDLGSAN
                     GTFVEDKLVESVQTLQPGDRVRFGHIGFYLVEDIATLPPPRSSRVVTTTIKPPMASQA
                     PARPLATSADEDFAEERTDVGLPEMRFRLHEPTGGGGGLIEVDGKQVQLTTTQFELMA
                     LMIKRMASEASQPEVVRGFVRSSELIADLSWDTREPSENHVKQLVRRVRRALLKSDIG
                     DLIESRHRFGYRLRAIPKIA"
BASE COUNT         1259 a         2508 c         2332 g         1293 t
ORIGIN      
        1 ggcgaccttg ttgcgaaccg cagccgcatg ctcgaccgcg acgatctcgc gcaggatgcc
       61 cggcgtgtgc cactcgcatt ccggaatcac gccggcatcc actccgccct tcggcggctt
      121 catcttgtcg cagctcttgc ccgcttcgcg cacgcccatg tccggcggcc cgaggatgag
      181 aatcgacgcc gtcggcgcgg cggcgcgaat gcgcaggatc gtctcgtcgt agtaacgagc
      241 catatcctcg agcacgagct cggggtcatc ggcttcgttc gtgccgtatt gcaggatcac
      301 gagccgcgga tcgcgcgtgg cgagttgctc tccgatgatc gaccagtccc acgagcgaag
      361 actgccgagc cttcgaccga cgacgccgag tgcatcgatg atcacgcccg gcttcacgtt
      421 ctccatcacg acgccgaaca gatcgacggg gccaccgccg ccgtgctcga gcgtcagctt
      481 gtgcgctcct tccgcgacgg ggacgacttg ccgcgcagga tgcggcggct cgatcgcggt
      541 cgtcttcgtc gagagatcga gccacttacc gtcgtcgacc ttgtagcgaa gcaggccgtg
      601 atcgggcgcc gcgtaataga gaatctcgaa ctgcgcggtg gtctttcccg acggacaatc
      661 cgcacacgtc tcgacccaca gctgcgcgcc tttcttcgtc cccgacacgc gaaggcccgc
      721 gatcccgaac ggctcgctgt cgccgcgctt tccgccgacg gcgccggtcc acgtgccggt
      781 gcggccgtac ttcacgtcgc gctggtagta atgcttcacg ggcggcttgc ccgccgcgac
      841 gacgccgcgc cccgcatcac caaatcggcg ctgccacacg agccgcagcc ggccggtcat
      901 cgcatcacct gcggtgtgcg aatcgccgaa cacgatcaca tcgacgcgac caccgggctc
      961 gcccgcttcg gccgccgcca gcgccgtgaa caagtagtcg agactgcccg gcgagacatc
     1021 cagagcgccg cgctcttcgg gcgtgagctg tggagagctc gcactcgcgg gtaacgcacc
     1081 gaggccgctg tcctcgacgg cgaccggcgg gtgcggcgtt tgcgatttgt gcgcgcgctc
     1141 gccatcgcaa ccgatctgac acccgaacag cccgccgaga gcgacgagcg ccagcgccaa
     1201 gctacttggt cgcatagctc ttcgatccag cgagcagcga gtcgaacagg ccgtccgcca
     1261 tcttgctggc accggcgtgg gtgagatgga tgaagtctcc gccgatgagg tgcttcttcg
     1321 accacttcat cgccgagcct ttgccgccca tccagtcgta cgtcgaatag aacgcacagc
     1381 ccgacgcgtg cgccgccttg cgctgtgcat cggcgatcgt tcgcatcacc ggcttcgact
     1441 gatagccatc gtctttggcc tcggcctgat cgctcggcga cacgacgaga cacgacgcat
     1501 cgggacgcgc cttgcgaaac ggcacgagca tcttctcgta ctcggcttga tacgtcttct
     1561 gcgtcgagtc gccggccttg agccacatcg cttcgttagc gccgatcatg atgatgatca
     1621 gatccgcgct gcgatgttcg agctccgtct tgaagtgctc gctctgcgac gccgcaaagc
     1681 tcttcgtgtg aacgctgacg atgccgaagt tgtcgacgac cgcaccggtc gcattctcga
     1741 gcgacacgcc gaacagccgg gtcttccctt cgccgcggat ctcgaacttc gtcgcgcctg
     1801 ccgcgatact gccgacgaga aatcccggtg ccttcgcatc cgccagcgtc gagatgctcg
     1861 cgatgtctgt tccatccgcg gcgatcgtcg tcgtgccgcc tttgggctgc gccaagtagt
     1921 agagctcggc gctcgttacc ttgccgttga cgagcttgat cgtcgagcgt ccgctgttgg
     1981 tctcgaccgt cgcgccgcca gggccataga agccgtcggt gacgtggttc gtcgaaatcg
     2041 cgtagctgag aaagttgtca ccgccactgc gcgtgatcgc gtcgtgatga cagaagcgat
     2101 gcggcggcag gatgtacacg aagccaggtc cgccgtcgcc gagctcggct tgcagacgcg
     2161 agcgcagccg gcccggaagc tcgtcactcg cggagaccga gtcgccgtac cacgacacgc
     2221 ggagcgcgcg accgagcttg gcgttcttcg cctcggagac cgcacgatag aacccgtcca
     2281 tcgcccactt cgcgcacttc tcttccgtgc cgtcgatgca cacgtcgtcg agtgcaccga
     2341 tgtcgtggct gccggcgggt ggtgtcggca acgcagcaac gaggtcagcc tcttcgacgg
     2401 cgctcgcttg atcggacttt gcgacgatga ccggacccgc gcccgccgct tccggttcgc
     2461 gcttcgtcgt gaggcgatcc gcgagcgccg agaagtttcc atcgagcaag aacagcgacg
     2521 ccgcgaacac tccgacgaac gtcacgagcg tcggggctac tgaccacccg cggtcctgcg
     2581 cagacacgcc tgaaaggtaa gcgaacgaat gcagcttctc aacacactct cgagcccgcg
     2641 ttggctcgct gctcgcaatg acaggcgatc acctcgggag ggtactgatg cagaagtcct
     2701 tgcgaattgg ctttgccctg tgtgtgcttg tcggctgcgg cgacgatgcc gcgacaaccc
     2761 ctgattctcc aaccggtaac cggccgccgc ctcgcgctat ctccggcggt ggcatcggcg
     2821 acggcgccat cgacggtgtc gtgaatctct acgtgatgga cgacgccaca cgaatgccgt
     2881 tggcgaatgc gacggtccgc gtcggcactc ttgacggcac cacggatgcc accggcctgt
     2941 tcgtcgccga aggcgttgtc ggtccgcaga ccgtcctcgc aaagctggcc ggctatcgct
     3001 cggagatgtg gatcggcgcg aacggcgcga acatgacggt gtcgctcaag cccgacgtcg
     3061 acccgacgcc agcgcgcgcg aagctctcgg gctcgatcac cggtttcgac cagctcacgg
     3121 taccgacggg gcatcgcaag gctgcgatcg tcacgtactc gaacgacgac aaggccggcg
     3181 acgcggccaa caacatcgac accgcgaacg cgacgaacat ctgcgacgcg ggcaccggca
     3241 tgccgtgcaa cttctcgatc acgacgcgca ccggcaccgt cgcgctcatc gcgatcgtct
     3301 acgaccacga tacgaagaac acgctcacgc cgaacgacga cacgtacacg ccgatcacgt
     3361 gggcctacaa gaccggcatc tccgtcgccg gcggtgtcga tcaaacgggc atcgcgctct
     3421 cgctcgtcga tgcggctgat ctcgcgaacg tcatcgtcga cttcggcacg ccgccggcgg
     3481 gcatggcgaa catcgcggcc gttatcggca tcgaggtcgg cagctcggga acgatgcagc
     3541 tcgtgccttc gtttgccacg cagtccgcgc caacggtgct cgccccgaag ctctcggcga
     3601 tcagcggcgc gacctaccgg ctcacagcga tcgcgcagaa cggcaacacc gagctcgacc
     3661 ccggtagctt cgttctgcat cgcgcgcaga cgtcgacgtc gctcggggcg ggcacctggc
     3721 tcggcgttcc gagcggtgta acgctgaccc gcacgactgc atcgtggact gcagtgcccg
     3781 gcgctgcgct ccaaggcgtc gagtacgaca tcgacaagac gcaccacgtc ctctcgatta
     3841 cgtcgttcga tgggtctacc tccgcgacga ttcccgagct cctcgcattg ccgagcagtc
     3901 ccctcgtcgg gagggtcacg gcactgcaag gcaccgtcga tctcatggat ttctcggtcg
     3961 acgatgtgtt gaccaagatc acgggattct ccgcgcaacc gctgcaaatc aactgaccga
     4021 gcgtctgcta ccattggagc ggcatgcggt ggctgctcgt cgttacggct gcgctcggtt
     4081 gcggccccac cgcatcgcaa tcgccgcagc cgagctactt cacgatgaac ggcccgagcc
     4141 cgctgctcgc gccggccgct gcgtcctacg atccgacgca agcaggcact gtcgtcgatc
     4201 gaatggtcga cttcgcgttc gacggcggtg acatcgagtt cgagatgcgt cgcgatggcg
     4261 ggcgcgtgat cgaagttgct cgcaatcact acatggtccc ggtcgtcatg cagtggaccg
     4321 ttggcgacct cgacaatctg ctgccgagcg gcgcggtctc tggcgtcgtc gtgctgcctc
     4381 cggccgatcg accgaatggc acgggtgccg gcgtcctact cacgacgtac aacctcgacg
     4441 atccgcgcgc cgcatatcgc cggcagctca acatgcacgc tcgcttcggc gatcccaatg
     4501 cgcgcccgca gccatacgcg tatcggcttc cgttcaaggc cggaaagacg ttctcgatcc
     4561 tgcagggctt ccacggcacg ttctcgcatc gcggctcgaa cgagtttgcg gtcgacttcg
     4621 actgccccgt cgcgacgcca gtgctcgcaa cgcgtcccgg aatcgtcgtc gccgcgaatg
     4681 cagccgcgca gggctcgggc acgacgcccg acttcctcga gtaccgccgc acgaacttcg
     4741 tgctcgttct ccacgacgac gggacgctcg gcgaatacat gcatctcgcc gcgagcggag
     4801 tgcgtgcgag cgtcgggcaa aaggtcgagc gcggggacga gctcgcgcta tcgggaaaca
     4861 ccggctactc gtcgacgccg cacctgcatt tccaagtgat gaccgcggcc ggcgacggaa
     4921 tttccgcgca gtcattccca ttcgagctcc agatcagcgc ccacaaggtc gaaccaccgg
     4981 ttcaaggccg ctcgtatccg agctgggaat aagaggcgcg ccgcggcgtc cattgcgata
     5041 atgggcgcgc gatgggacgg gcatggttta ttgcggtcgg gttgacgctc gcgtcgtcag
     5101 ctgccgctgc gccgctcgaa gcaccgcagt tcacgattcg cccgcaaggc gcgatggcgg
     5161 cattcaccgg cgaaaccgtc tcgatcacga tcggctggcg tccggtcgca caggccgcga
     5221 agtatcgact cgcgatcacc gacgcgaaga atcagacgac gcagatcgat gtgacggggc
     5281 tgcgccacga gctgacggac ctcgcgcccg gcaagtacca agtcaccgtc gccgcgctcg
     5341 acgcgtccgg catggcggga cctaccagtg agctgctgta cctgagcgtg atcgagatgc
     5401 gcgcaattcc tccgggcagt agccaatcca tgccgccgca gcgtggcgcg tatgcggttg
     5461 gcactcgctt cgcggtcgga gggatgcact gcgaattctc cgacgcgccg atcgacgatc
     5521 tgctcgtcgg tcccgagaac gaagtgcgaa tgccgatcgc gggcgtggcg cggctgcgct
     5581 gtgcaggcat ccccggctac ctcgagaaac aggtcgtcat cgcgcccgtc gcgatccaga
     5641 ccgtgacgcc agcgacccgc ggggtgacca cggatgtcca cgtgacagtc gcgtcggtcg
     5701 cgttcttggg agagcagctc gatgtcgaag cgattgggga tctgacgctc ggcgacgcgc
     5761 agcggacgga cttcggcttc gaggtgcccg tccacgtcgc cccgactgcc acctcggcgg
     5821 cgctgagcat ccagacaggt gacttcgagc tgggccgcac gagccttcaa cttatcgatg
     5881 cgccacctcc cccgccgccg cccgtgcgca agccgctcga ctggcgagca ctcgatctcg
     5941 gaggtcatgc gggcgcgttc gtcccttcgg atggcggcca cgctccgacg atcgggatgc
     6001 cgaccgatcc ggcagacgtg gtcagcggcg gtccgcttgt cggacttcgc ctgggcttct
     6061 tcccgacgag gcgcgtcggt ctcgagggcg aaatctctct gatcgaggcc ggtcatggcg
     6121 accgcgaagg tacgagccac gtcgtcgccg gtcgggcgca actcgcggtt cgtgccgttg
     6181 agagcggaaa tctcggactc cgtgtgatcg ccggcaccgg gacgttcacg aacctcagcg
     6241 agtccggcac cgcgcgcgtt gcgacccagg gcgagattca cgcgggcggg gcactgacga
     6301 tcgagacgag cccgaatctg tggctcagat tccaatttgt agacgcggtg acgaccgcac
     6361 gcgatgacgg ctactcgcat tgtttggagc tccaactagg ggtcgtgacg cgcgttggcc
     6421 gccgcgactc ctggtaggat gattcacgtg aggtgcccgg tatgttccga acgaccggcg
     6481 gaggttggag tcctctgcga agagtgtcgt gacgagctcg atagtccgat caagatcagc
     6541 caagagcaga tccttgctcg cgtgcaaacg ccgacgaacg cagccctgat cgatctgtgg
     6601 ggtcgtcccc atcagctcga cgcccacgca cgcatcggcc gccagatcga agcgggaggc
     6661 gtcgccgtgc tcgagccgag tgtctcgcgc ctgcatgccg agctcgtgtt cgagggcatc
     6721 tcgtggacga ttcgcgacct cggttccgca aacggcacgt tcgtcgaaga caagctcgtc
     6781 gagtcggtgc agacgctgca accgggcgac cgcgtccgct tcggccacat tggcttctat
     6841 ctggtcgaag acatcgcgac gcttccgcca ccgcgctcgt cgcgcgtcgt cacgacgacc
     6901 atcaagccgc cgatggctag tcaggctccc gcacgtccgc tcgcgacgtc cgcggacgag
     6961 gacttcgccg aggagcgcac cgatgtcggc ctgcccgaga tgcggttccg cctgcacgag
     7021 ccgaccggcg gcggcggcgg tctcatcgag gtcgacggca agcaggtgca gctgacgacg
     7081 acccaattcg agctgatggc gctgatgatc aagcgcatgg cgtcggaagc gagccagccc
     7141 gaggtcgttc gcggcttcgt tcgctcatcg gagctcatcg cggatctgtc gtgggacacg
     7201 cgcgagccga gcgagaacca cgtgaagcag ctcgtgcgcc gggtccgtcg agccttactg
     7261 aagtctgata ttggcgatct catcgagtcg cgccaccgct tcgggtatcg ccttcgagca
     7321 attccgaaga tcgcgtgaag ttgttaccgt cggtgaatgc gacgtttggc attcgccttg
     7381 gtaatggctt cg
//
LOCUS       DATLBZ010000272        22643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1046983, whole genome shotgun sequence.
ACCESSION   DATLBZ010000272 DATLBZ010000000
VERSION     DATLBZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22643
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046983"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VMZ53_14235"
     CDS             <1..480
                     /locus_tag="VMZ53_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29667.1"
                     /translation="SMHGGIVGGVVGLTPRIASSAMALVTAATERDHSFTAFTDGITP
                     LSISPRQRLDDVVNEVARLPFGGTDCALPMLWAQKHRVDVDTFVIYTDSETWAGHVHP
                     SQALRSYRDARGIPAKLVVVGMTSNGFSIADPDDAGMLDVVGFDSSTPPVIADFARA"
     gene            556..629
                     /locus_tag="VMZ53_14240"
     tRNA            556..629
                     /locus_tag="VMZ53_14240"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:590..592,aa:Asn,seq:gtt)
     gene            1436..1505
                     /locus_tag="VMZ53_14245"
     tRNA            1436..1505
                     /locus_tag="VMZ53_14245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1466..1468,aa:Arg,seq:gcg)
     gene            1742..1812
                     /locus_tag="VMZ53_14250"
     tRNA            1742..1812
                     /locus_tag="VMZ53_14250"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1773..1775,aa:Trp,seq:cca)
     gene            1907..1980
                     /locus_tag="VMZ53_14255"
     tRNA            1907..1980
                     /locus_tag="VMZ53_14255"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1941..1943,aa:Pro,seq:cgg)
     gene            1994..2065
                     /locus_tag="VMZ53_14260"
     tRNA            1994..2065
                     /locus_tag="VMZ53_14260"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2026..2028,aa:Ile,seq:gat)
     gene            2707..2779
                     /locus_tag="VMZ53_14265"
     tRNA            2707..2779
                     /locus_tag="VMZ53_14265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2740..2742,aa:Leu,seq:cag)
     gene            2800..2872
                     /locus_tag="VMZ53_14270"
     tRNA            2800..2872
                     /locus_tag="VMZ53_14270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2833..2835,aa:Leu,seq:gag)
     gene            2918..3475
                     /locus_tag="VMZ53_14275"
     CDS             2918..3475
                     /locus_tag="VMZ53_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HUS29668.1"
                     /translation="MTHVESTRTLLLTQGYEPIQIISWQRAITLLALDKVDVVEQYDA
                     IIRAPSVVVNVPAVVRLRKAFRRHAKPVKFSRVNIYARDNYRCQYCGQKCTIDELTYD
                     HIVPRSKGGRTTWENIVSACYLCNRRKANRTPVEAGMKLLSVPARPTWMPSVQIRVSA
                     RSVPDAWRDYVYWTAEIDNEEGGSD"
     gene            3539..3611
                     /locus_tag="VMZ53_14280"
     tRNA            3539..3611
                     /locus_tag="VMZ53_14280"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3572..3574,aa:Gln,seq:ctg)
     gene            3709..3783
                     /locus_tag="VMZ53_14285"
     tRNA            3709..3783
                     /locus_tag="VMZ53_14285"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3740..3742,aa:Asp,seq:gtc)
     gene            3785..3860
                     /locus_tag="VMZ53_14290"
     tRNA            3785..3860
                     /locus_tag="VMZ53_14290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3818..3820,aa:Val,seq:tac)
     gene            3908..4849
                     /locus_tag="VMZ53_14295"
     CDS             3908..4849
                     /locus_tag="VMZ53_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29669.1"
                     /translation="MSTGRADIARMVLREPGVSDAAHYGKRVRWLAAVHAALFVLSLV
                     GFALLIWKGAFFVTLSQRSNVETLTIVFFLLFFGYFAVVSSPGALGALRILALRGDEK
                     KKQANLEKRGRTPGAGAAFDKAVELEGRLGEPFTIEIRDDIGSLGRLQLDGVKITHLD
                     SFRDGSNTLLAYVEHKIEQITGHEVEIVQWRSTDDEALAQFVATSHAFRALGRKLESD
                     VWPTVVLTEEQRQRLEDEVAAICPALRTEALLPDWEFEGEHKLPIIPEPLGIISLSRK
                     EKRVDTLSAMVAAVSVVAIVVALIIFFVVRPPWLPGR"
     gene            4831..5133
                     /locus_tag="VMZ53_14300"
     CDS             4831..5133
                     /locus_tag="VMZ53_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29670.1"
                     /translation="MAARSLIFILLVACSGPQPKVEHATASPSPVPGATRVAMDIVNS
                     GGEGTVAIHVELRDARRIIRAERTLAVQGKQTIHFETDVETPPGEYRVAASAQYPD"
     gene            5186..5869
                     /locus_tag="VMZ53_14305"
     CDS             5186..5869
                     /locus_tag="VMZ53_14305"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HUS29671.1"
                     /translation="MWRMWLTGVVMLAAGACGRSGFGAVIDAQSTAADGRVSTSDADQ
                     IAIDAAAGTVIRYSIADTTIRMSGGVPSGNDNVVICGQYSSNLDDTRPLLLLDLSGVT
                     GPVTNATLRMYMTLSGGSSPIDIGIYRVTVAWTEAGASWTRPAPSQTWTTAGGDTAPL
                     LYAHTTITPAMFAFYDWNITSLVNEWLTGTPNFGLQMRYVTVPATPSNVGLRSREYPA
                     SQPELLITP"
     gene            5953..7596
                     /locus_tag="VMZ53_14310"
     CDS             5953..7596
                     /locus_tag="VMZ53_14310"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HUS29672.1"
                     /translation="MRRALFVLTLLGGLASCHHGGVPASAKTKPKLVVVVVIDQWPSW
                     VFQKQRDLFTGGIGHLVKEGAVVPEAELPYGNPFTAPGHAAISTGATPRVHGIVGNSW
                     WRRDEGRERPAEYDPDAPVFSVGDAYNGATMDAGVSAKMLRVDGIADALRAQTNGAGK
                     SVAISFKPRSAVLIAGRKPDLAIWYDAGAGGMTTSKAYVDTVPAWLQQMAKDSPVNKF
                     FTATWDARDPVMYGEVLDVPDDAKGEASEHGLGARFPHQLATSEHPEHAIVETPFADR
                     VVSRTVAVAIDAMELGRDEVPDLLAISYSAHDYAGHNWGPDSWEVLDDTMRLDAELGE
                     LFESLDSRIGKDNWALVVTSDHGATPVVERARIRSARRIPPVEVEQAADKAIAQLQGG
                     GPWVATQTSNTIYMTAKFAALPEAQKNESLDAAVEALKHLPGIAVAGRTDRFSKDCTV
                     EKDIYRVMCNAYVPGESGELYAVATTGSLISEYKAGTGHDAPYDDNRHVPILIKAPGV
                     KAQQGQGTLLQVAPTVAALLGIDPPSAARAPTLFKIQKR"
     gene            complement(7593..8417)
                     /locus_tag="VMZ53_14315"
     CDS             complement(7593..8417)
                     /locus_tag="VMZ53_14315"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HUS29673.1"
                     /translation="MPPVDRCYVYQPDFIDPASRAEILAWLATLHPLWEQRYSTKRPP
                     PAGKTQRGLLRPVYWLGNWQFACLGYYEPPKRTKHVAVAAEPFPPVLARLVAAIEKRV
                     RHGFPQQTVPRQWHLNTCLVNFYGSREQDGKWSDAARVGEHRDFEPGPVASLSLGERA
                     RFQFVRRGATESTPPVRTEWLDDNSLQLFAGPRWKDDLLHRVQRVENKREVDLPPKIA
                     GFRTRRVNFTFRYVPDEHVTPFAQLPAAAREDVRDYMAELAQHSPFFAKALARGGS"
     gene            8467..9081
                     /locus_tag="VMZ53_14320"
     CDS             8467..9081
                     /locus_tag="VMZ53_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HUS29674.1"
                     /translation="MLRWLYRPQCIACAKPSSSELCADCAISLVPLTSQAAAPAPLLG
                     IASAWAFGGALASAIRRLKFAGATHIARTLAPLWAPVIEAAVIEHDAIVVPVPLHWRR
                     RARRGFDQSWLLAAHACAFAKLPPPVAALRRIRHAKAQSTLPAAERSENVAGAFRLRR
                     DIAGRAIVLVDDVSTTGATLAAAARPLIDGGASLVVGVTVARAE"
     gene            complement(9082..14517)
                     /locus_tag="VMZ53_14325"
     CDS             complement(9082..14517)
                     /locus_tag="VMZ53_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HUS29675.1"
                     /translation="MTIRRWAQLAAFVLLFFVTRNASAIVVGVCNSTNCSGGKTAAVG
                     NVVVNATGTNSDVYLTSDTQNYNADLVISGCTGTGTGTFTARIGGASTNIQLKNNQNN
                     VVDVAYTPTTRGGRSCTVNVYPAGTTTGSLGSFTITATGVAPVISTVASLAFGTVRYN
                     DATPLSTHTATLNLQISNTGDAGQPLQINAITLTGAGAGDYAVSSTPALPATVNPGTP
                     VTWVITFDPIAAGASSATINIDSTDPVTATKTVSVSGTGGTAVIGVTTAINFNTINQG
                     TTSTTQNISVSNNGAGTKATMGVTQAALVNNTAGWFKFNQAGCVGSTTTCTLAMAIVG
                     TPGTVGVQCAPPAGASGTQTASVQFTSDADDNSANTTSLSCTAGRADIVVTTTPLAFN
                     NQLINTTSTGQTVMIQNTGNATLTYNVGLAGVDPTQFILTGAAGCTSGCNVSAMSSVL
                     VTVQFRPTSVGAKNAVLRVSPTNDPDTAGPIDVGLSGTGIAPLAAYNVTSMAFGNIEV
                     GATATAQTLTVTNNGTSDLTVSQAQLSAGATDYVVQTGTTGATISIVIPPTMTATWTI
                     ACKPTQQGSRAGTFHVNTDSNNTLTGQDIALSCNGQKGNLTITASSFDFMQKLEGSMT
                     PQTFTLTNTGNLPVTNINYAMTGTGTGYSILSPTFPIASLGANASVTVTVQFYPLNGN
                     DGGALTYTFSGVWGSGNVPTSTTLSLNGDGLTTGYDTSPSNPYPVDWGDIRFDTPTNT
                     TLNIVNTAGTAYNIMSLSITPDMNTASSEIAIIQCRHNGVAAAATTNGCPAGTTYAPS
                     SGANDTTVVTVQCKPNNRIGMVGGTLTVHTSLPLNPDRVVALKCNSTTAVLDLAPGNV
                     VDFGPVDLDITPTTKTKTVTLKNTGAAMMNIGAVSKTTTGPFTFTATPTAAVGPNGTY
                     SFDVTYTPTAERAPNQPETATVVVPLTGAIGTPGSATVMISGYGVDRHIGVAPAPVFP
                     NTFKNPGDKAPILPVTITNNGDANLNVSAVMLSDAPIWTITNPDPVDVSGRQSYEFLV
                     KFEPVTAGKAPTGHMVIMNNDNGKPVVSVDLDGIALDRLVEFGPNEINLGYVGIGIST
                     RLSEAGVTLEATPTTPVLSLDVTNHEMTEQFELSAMTITGGDGAFRVLDLTDDHEIGD
                     TETMQLTPQSTHKFDVVFTPTHEGDFVADVNLFLDNADTSHSTVRLRGRGLYVDVRGG
                     GGCSTGGNSGGGALLLVLGALLVGRRRKARGLLGLIGVVGAASAAHAETRNLDTTLFD
                     PTPATQVTALQSQSADVGKDGDYAISALLTYADNPLVLGTSQNTYTAVDNRSVLVLGG
                     AYAFGTRFEAGLRMPLLLQNGQATGEQNKMFQVVQVDSVTRGDATVHAKMNGGALEAL
                     GGDLSYGGAFALSIPTRSGSEFAGTNMPQARLLGLATLTKGRLVLQMNAGAIIRSKDR
                     YSSLVQRSGGTFAVGGTWRAANKVWIGGEVFGNLIPGGKTGDPEGMEMYGPPELSSSI
                     EGLAELTYRMSRTATLGVALGRGITSGVGTSNARGALMLSFTPGADAVVALPKKIHIE
                     GPNDKDGDGVKDKLDACPNEAEDKDLFEDGDGCPDVDNDNDGISDEKDKCALDAEDRD
                     RFEDEDGCPERDNDNDGVADDQDKCPVAAEDKDGFQDTDGCAEADNDNDGLVDKADKC
                     PNEPESINGNADDDGCPDRGDALVVLSPDRLDLLDNILFEGGAKIARGSKNILSQIGA
                     TLRAHPELLRIRLTAHVQPTGNPKNDLALSEARAKAVREWLVDWGIDPLRLQSSGFGS
                     SKPLVDPKSKGAREINDRFELIVLERK"
     gene            14683..16464
                     /locus_tag="VMZ53_14330"
     CDS             14683..16464
                     /locus_tag="VMZ53_14330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29676.1"
                     /translation="MTDDGSAPTIPDTQAPTHGSTPSITPARSPDPRYGLRTLLGKGG
                     MGEVWLAHDSRIDREIAIKLMRGSSDPDSAARFLREARVQGRLEHPSVVPVHDLGTEP
                     GSPFFAMKRLTGTTLADVLAERDHTSTIPRRTLLQKFVDICLAVEFAHQRGVIHRDLK
                     PANIMLGDFGEAYVLDWGLARMAGDSSESIVRPSDRAGDSGGQTEVGAMLGTPGYMAP
                     EQMRGEQIDPRADVFALGCILFEILAGQAAIPRAHPYEVTLASPHYRPSERAADVPPE
                     LDAAVVRATAALPADRHDTARALADEVQRFLDGDRDLERRRQLSMQHTERARQLAGNT
                     DIESRAEAMREAGSAIALDAGNREAHAVLMQLLLERPKELPETVKKKIDEDRQRAFQP
                     VIRGMVFVYAFALVTALVVRQWSAPGDWRLALLALENGVLAILLSVAWWRRWYITPSL
                     FTIGALLHAAILTTVGFAVGPLLLLPVLLFGSLPVLMVAPIIRVPIAVFAAHIVAFAV
                     PLALEWLNVVPSSYRLDGNLLVLEPGVGLPASVTVAGVITIVLLQLVGLSTVLDAQRR
                     EQDSAQETVQLQSWQFAQLVRSSGSST"
     gene            16487..16837
                     /locus_tag="VMZ53_14335"
     CDS             16487..16837
                     /locus_tag="VMZ53_14335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HUS29677.1"
                     /translation="MEKTSSGLEYEDTQVGTGPSPQKGQTCVMHYTGWLWVNGAKGSK
                     FDSSVDRGSPFEFPLGMGRVIKGWDEGVASMKVGGKRTLRIPPDLGYGQRGAGGVIPP
                     GATLVFDVELLGVR"
     gene            complement(16762..17169)
                     /locus_tag="VMZ53_14340"
     CDS             complement(16762..17169)
                     /locus_tag="VMZ53_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29678.1"
                     /translation="MTRILLVLALAACGGGTQAGPVTAHPEPPPPTRIESTPPVTKAL
                     VAEECQQLAKHVVQLSVKERPEDQQPSAEDLDKLDLRSTVELCLTLPRGTYDCAMAAS
                     TTTAMAACDQRTPSSSTSNTSVAPGGITPPAPR"
     gene            17197..18012
                     /gene="uppP"
                     /locus_tag="VMZ53_14345"
     CDS             17197..18012
                     /gene="uppP"
                     /locus_tag="VMZ53_14345"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HUS29679.1"
                     /translation="MPLWFAALLGLVQGLTEFIPISSTAHLRVVPALLGQPDPGAAYT
                     AVIQLGTLVAVLAYFAKDLAHLPAAMMRESGTFEARLPWLLAVGTIPIVIAGLVLKKH
                     VEGDLRSLWVIGGALLTIGIVLALVDRHVAGRPSFRPLADLTYVDALIVGLAQTMALV
                     PGVSRSGSTMCAILLLGFSRSDSARFSFLLSIPAIAGAGIFEARDAFRTLGSSAVPAI
                     AVGMIVAAVTGYVAIAWFIKFLGQNSMMGFAVYRIACGAALLTALAAGFLRRV"
     gene            18066..20246
                     /locus_tag="VMZ53_14350"
     CDS             18066..20246
                     /locus_tag="VMZ53_14350"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS29680.1"
                     /translation="MRITRLVVLSLITLGMLATAALSAPCPTTTPGKYPVKIDSAPQG
                     AQVYINDKTCLVGNTPWSGKLAKGTHTVIIEATGYEPMSRQFNVAAVRKTQELFVPLV
                     KKADPPKIDVKADADPKGVAGAQVWVDGEMKGAAPISLTVTEGRHLLQLKKDGYETYE
                     TWVPTSANNTATLLPQMKEVPKPKFGTVVVEADVPDAEVYIDGNKHPDNTPAMIANVI
                     EGPHIIEVRKPPSLPWKQTVQVTADKSTKVRAELQATMHGGVGVVRVLSDAQGARAFI
                     DGTDMGPVPVDIKDVKAGEHIIQVKAPGMKTDEKKVTVAAGSSQIVKFDLNAETPADQ
                     GTLKVVSMVPEAQVFIDGAAVGKVPVDKKLSAGEHPVVVRLEGFKQFEQKVRVEAGQT
                     VTVQATLKPVGRLRILSTPAQAEVVINGVPAGKTPLDQEVETGETVIRIELAGFQPFE
                     QTLMIEGGKTQTLSRELAIAGLSEAELASQQRGLSSFGARTLPRGRSTVDFSGGYPYY
                     LTARINVGAGKIAKKFGFDAGVGVRTMFARNELGLGGRMMLADADPFSAGVFTDIWWG
                     SKLLDDSRRNGFTWDVGAAASLTALSHVTITGRFYFDIWSDRHCPELKTGGTGGFDGD
                     PIAVCEAYAANTLTVDETMRINKLTTGDEATAWKFADRETGIRLMASVIAEIAVEQRW
                     NLFGILEGAPFQNERALFTSDFSHSMPSDSDFNLYLRLGASYKF"
     gene            complement(20247..20801)
                     /locus_tag="VMZ53_14355"
     CDS             complement(20247..20801)
                     /locus_tag="VMZ53_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HUS29681.1"
                     /translation="MGGEHDHLADNIKALRETRGLSQQQMAKAAGIPRATWTHLESGA
                     ANPTLAVLIKVAAALQVRLDELLASPRQPARFLKAAELPTRDRGQVLIRKLLPEPLPG
                     LDMERMTLPPGARMAGVPHTPGTREYLTCERGSVELAVAGQRYTLDEGDVVSFRGDQR
                     HAYFNPGKSTAIAYSAIAFAPTTA"
     gene            20903..21694
                     /locus_tag="VMZ53_14360"
     CDS             20903..21694
                     /locus_tag="VMZ53_14360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS29682.1"
                     /translation="MKIQGQGALITGASRGLGRALAEALAQRGAKVALVARDIRPLQD
                     VVATIRAQGGDAHAIAGDIAAKDAVHRIAGQAGGLVGEIGIAIHNASSLGPTPLRLLL
                     DTECEDLAAVLETNLVGPFRLTKILAGAMALRGAGTVVHISSDAAVEPYARWGAYAIS
                     KAAQDHLSRILAAELADTGVRILAIDPGEMDTKMHADAIPDADRATLQRPADVATRIV
                     QMIEDEVRAPTGSRLTAPTWAAAPTALTSVPPPGATPASTTEAKP"
     gene            22027..>22643
                     /locus_tag="VMZ53_14365"
     CDS             22027..>22643
                     /locus_tag="VMZ53_14365"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HUS29683.1"
                     /translation="MTVARADERVAITSFAELPSVLRAGDLVVVNDAATLPGSLRGQT
                     ERGEAIELRLSGPVERNRLVGVLFGAGDHRTRTEHRAAPPTIAVGEVVQFGVGDHRTR
                     TEHGTALPTIAVGEVPRFAAGDAPYRALHARVVATSGRRVELETRESGDELWQALYAS
                     GAPVQYAHRPKLLPLYAVQTAYAARPWAAEMPSAGRPLTWDVLIGL"
BASE COUNT         3770 a         7381 c         7453 g         4039 t
ORIGIN      
        1 tcgatgcacg gaggcatcgt cggaggcgtc gtcggcctga caccgcgaat cgcgagctcc
       61 gccatggcgc tcgtcaccgc ggcaacagaa cgcgatcact ccttcacggc attcacggat
      121 ggcatcactc cactctcgat ctcgccgcgg caacgcctcg acgatgtcgt gaacgaagtg
      181 gcccgccttc cgtttggagg aacagactgc gccctaccga tgctctgggc gcagaagcac
      241 cgcgtcgacg tagacacgtt cgtgatctac accgactccg agacctgggc gggccacgtg
      301 cacccgtccc aagctctgcg gtcttaccgt gacgcacggg gcattccggc caagctcgtc
      361 gtggtcggaa tgacgtcgaa tgggttcagc attgctgatc cggacgacgc cggcatgctc
      421 gacgtagtcg gtttcgactc gtcgacgccg ccggtcatcg ccgacttcgc tcgcgcgtag
      481 tgcactacgt gctcgatgtg aaggcgcggc tcgcgacagc tgccggcgcg gcgggaaacc
      541 tccgcgtcgg tatttggggt cgaagctaag ctggtgctag cggccgtttg ttaaacggaa
      601 gaatgttggt tcgattccaa ccgaccccgc tctcgttttg attttcgcgg gccgaaaggt
      661 agttggttac cgtaactata acgggcctaa ggtagcgaac ctggggcgac ccagttagcg
      721 aagcagcagg ctgtctcgac aaggcttcgg ctacagctca ttgcgtgccc actgccggca
      781 acgctcgctc gcaagagcga atgtccggta agagcgccga cgcgcaagcg tcacccaact
      841 ccacacactt gtccgcattt gtttccgtct ctgtcggcta acagaaggcc gcaggtttct
      901 cagccctgct gtacgggtgc aagtcccgtc agagaccccg cagtgatctc aggtgagacg
      961 acgatcgaaa gcgctaatca cgcccagatc cgttcgttag gcgcgaacga acgaagggtt
     1021 cgcgaggcgc cagctggtcg cttcgctcca gctgcgacac gtgcttcgca cgcgtcggcc
     1081 cgcttcgcgg gcgcgcctcg ctcttcacgc gcccgctgct tcgcagccgc gccgcatgct
     1141 gcgcatccgg cggctcgcgg tgcgagctgc gcgtgtggcg attccctcgt gctcgacgtg
     1201 agctccgcaa ttgctgggca ggtgccgtga ggcacagacg gttcgattcc gtcctttgcg
     1261 accgcagtga tctcaggtga gacgacgatc gaaagcgcta ataccgccta ggtccgttcg
     1321 ttaggcgcga acgaacgaag ggttcgattc cctccactgc gaccaaagct tctaggaagc
     1381 ctcgggctca cgaagcgaac agctcccagg agcctcgggc tcgaggagca gatacgcccc
     1441 cgttccaagg atgggacacg acagtgcgaa cgtcgttggc agaggttcga atcctctcgg
     1501 tggcactcct cgcgtagcgc agtcgcgctt cgctctatcg cagtccgtgc tgggcaccgc
     1561 cgtggctcat tcccgtgtgc gcgacggttc gattccgtcg actgcgacga ccagagatcg
     1621 aattcgccga aaggcgacag cagatcgact tcgcgcttcg tgcgtgaaga gcaaacgtat
     1681 tcgcgggtag ctcgcgcggt gtttcccccc attccgccgc gcgagccttc gcccgctatc
     1741 tacatcgcgc gtgttggtgc acggcctcga ctccaaatcg agagcggctg ggttcaaatc
     1801 ctaggcggtg tgcgaatcgt ccgtcccgct cgcaagagtg agacgggccg ccgtgtggca
     1861 gcagagccgt tgggtggtgc ccaacttttc agcacggcaa cttttacggg gtgtagctca
     1921 gcctggcaga gcgctcggtt cgggaccgag aggccgatgg ttcgagtcca tttaccccga
     1981 cgacgtgacg tgaccgcctg taactcagca gcagagtgct tgcccgataa gcgagatgtc
     2041 gaaggtgcga atccttccag gcggactgtg gtcgaggccg aagaagccga ggcgcgagga
     2101 tgtggccctc gatgaagtgg gtgcgagtcc catcgatcac cccaagggag tgttgacggt
     2161 aaaggaaccg atgctggccg taacccagtc gtctcgcgac caactccgtg caagtcggag
     2221 cactcccaca agatttagat gtcagaccat cgatgtacag atcctcaatg gaggacgcga
     2281 agtcgaaggt ctgtgcgcgc tgcgaggttg agaagccgat cgatcgtttc gcgcgtaagt
     2341 acgacgcgca tcagtcgtac tgcaaggagt gccagcgggc atcgagccag gcgcactact
     2401 accagcacaa agactattac ctcgcgaaga acgccagagc acgcaggcga aagagcgcgt
     2461 tcatcgaaga gcttcggaag caaccatgca ctgactgcgg aggagtcttc cctccgtacg
     2521 tcatggagtt tgatcacgtg caggagaagc gctggcccat cgggcgcatg tcggcgcaca
     2581 cgtggaccca acttcggtcc gagttggaac gcgtcgaggt tgtctgcgcg aactgccact
     2641 gcatccgaac gcaccgtcga aaaatgggct cgcgcgccga agaaccgcgc gctgtgtacc
     2701 aattttgccg ccgtagacca atggcagagt cgatgcgctc agaacgcatt aagtaggagt
     2761 tcgagtctcc tcggcggtac tgcccgtttg ctttgttgag ccgccgtaga ccaacggcag
     2821 agtcactagc ccgaggagct agcaagtgcc agttcgaatc tggccggcgg cacgtcgccc
     2881 gcaatttcgc gggccgttaa cataagggag gcatgccgtg acgcatgtcg agagcactcg
     2941 cacgctcctg ctcacgcagg gctacgagcc gatccagatc atctcttggc agcgtgccat
     3001 cacgctgctc gcactggaca aggtcgacgt cgtcgagcag tacgacgcga tcatccgtgc
     3061 gccgtccgtc gtcgtgaacg tcccggctgt cgtgcgtctg cgcaaggcgt ttcgtcgcca
     3121 cgccaagccg gtgaagttct ctcgcgtgaa tatttacgcg cgtgacaact accgctgcca
     3181 gtactgcggt cagaagtgca cgatcgacga gctgacgtac gaccacatcg tgccgcgctc
     3241 gaagggcggt cgcacgacgt gggagaacat cgtgtccgct tgctacctgt gcaaccgccg
     3301 caaggcgaac cgcacgccgg tagaggcagg catgaagctg ctgtctgtgc cggcgcgtcc
     3361 gacgtggatg ccgtccgtgc agatccgcgt gagcgcacgc agcgtcccgg acgcgtggcg
     3421 cgactacgtg tactggacag ctgagatcga caacgaggag ggcggctcgg actgacgcgt
     3481 gaaagcgcgc aggcacggaa cgccgctctc ggagctcgac ggcaatcgag ctccatttgg
     3541 aggactagtt caatcggtag aacgtgggtt tctgagtccc gaagttggtg gttcgactcc
     3601 accgtcctct tccgaacgga tcgtcatcat cttttgggga agccagaagg ctggagcact
     3661 gtcttgcaca cagagctttg cgggagcgtt acccgtcttc tccacgttgg ctccgtgcgt
     3721 ctagtgacgc gcgctgattg tctatcagac gcttggaggg tgcgatcccc ttcggggccg
     3781 ccaacgggac cagttcacct aggggtgatg tcgttcttac aaagcgactg cggtgggtgc
     3841 gattcccacg gtcctgacca gaaatttcgt agagccctgg catcgcctct gcacttacgt
     3901 actcccggtg agcacaggga gagcggacat cgcacggatg gtgttgcgag agccgggcgt
     3961 ttcggacgca gcgcactatg gcaagcgtgt tcgatggctc gctgcggtcc acgcggcgct
     4021 gttcgtgctg tcgctggtcg ggttcgcgct gctgatttgg aagggcgcct tcttcgtgac
     4081 cctgtcgcag cgctcgaatg tcgagacgct gaccatcgtg ttcttcctgc tgttcttcgg
     4141 ctacttcgcg gtggtgtcgt caccgggcgc actcggagcg cttcgcattc ttgcgttacg
     4201 cggggacgag aagaagaagc aagcgaacct cgagaagcgc ggccggacgc ctggcgcggg
     4261 cgctgccttc gacaaggcgg tcgagctcga ggggcggctc ggcgagccgt tcacgatcga
     4321 gattcgcgac gacatcggga gcctcggcag gttgcagctc gatggcgtga agatcacgca
     4381 tctcgattca tttcgcgacg gctcgaacac gctgctcgcg tacgtcgagc acaagatcga
     4441 gcagatcacc gggcacgaag tcgagatcgt gcaatggcga tcgacggatg acgaagcgct
     4501 cgcgcagttc gtcgcgacgt cgcatgcgtt ccgcgcgctc ggtcgcaagc tcgagagcga
     4561 tgtctggcct acggtcgtgc tgaccgaaga gcagcggcag cgcctcgagg atgaggtcgc
     4621 cgccatctgt cccgcgttgc gaaccgaagc gctgcttccc gattgggagt tcgaaggcga
     4681 gcacaagctg ccgatcattc ccgagcccct cggcatcatc agcttgagtc gtaaagagaa
     4741 gcgcgtcgat acgctgtcag ccatggtggc cgcggtgtcc gtcgtcgcga tcgtcgtcgc
     4801 actcatcatc ttctttgtcg tgaggccgcc atggctgccc ggtcgctgat cttcatcttg
     4861 ctcgtcgcct gttcgggacc ccagccgaag gtcgagcacg cgaccgcgag cccgtcgcca
     4921 gtacccggcg caacgcgcgt tgcgatggac atcgtcaaca gcggaggtga aggcacggtt
     4981 gcgattcacg tcgagcttcg cgatgcgcgc cggatcatcc gcgccgagcg gaccctcgcc
     5041 gtgcagggca agcaaaccat ccatttcgag acggacgtcg agactccgcc gggcgagtac
     5101 cgcgtcgccg caagtgcgca gtaccccgac tgaccggatt gaatgacctc tgcacgccgc
     5161 ccaaatgtcg gattacgatg gggacatgtg gcggatgtgg ctgacgggcg tggtgatgct
     5221 cgccgcgggt gcgtgcggcc gcagtggatt tggcgcagtg atcgatgcgc agagcaccgc
     5281 cgccgatggc cgcgtgtcga cgagcgatgc cgaccagatc gcgatcgatg cggcggcagg
     5341 caccgtgatc cgctattcga tcgccgacac gacgatccgt atgagcggcg gtgttcccag
     5401 cggcaacgac aacgtcgtca tctgcggcca atacagctcc aacctcgacg acacgcgccc
     5461 gctcttgctc ctcgatctca gcggcgtcac cggacccgtc acaaacgcga cgctgcgcat
     5521 gtatatgacg ctctccggtg gctcctcgcc gatcgatatc ggcatttacc gcgtgaccgt
     5581 cgcgtggaca gaagctggcg cttcttggac tcggcctgct ccgtcgcaga cgtggacgac
     5641 cgccggcggc gatacagcgc cgttgctgta cgcgcacacg accatcacgc ccgcgatgtt
     5701 cgccttctac gactggaaca tcacgtcgct cgtcaacgag tggctgaccg gtacaccgaa
     5761 cttcggactg caaatgcgat acgtcacggt gcctgcaacg ccgtcgaacg tcggcctgcg
     5821 cagccgcgag tatccggcta gccagcccga gctgctcatc actccgtagc ctccgcacgt
     5881 agatcgtgac ccgggaatcc gaatcctggc gaggtggtgc gattccgccc ggcggcactg
     5941 tagagtcgcg gcgtgcgacg cgcgctgttt gtgctcacgc tgctcggagg actggcctcc
     6001 tgtcatcacg gcggcgtacc ggcaagcgcg aagacgaagc ccaagctcgt cgtggtcgtc
     6061 gtcatcgatc agtggccgag ctgggtcttc cagaagcaac gcgatctgtt caccggcggc
     6121 attggccacc tcgtcaagga aggcgcggtc gtgcccgagg cggagctgcc gtatggcaac
     6181 ccgttcaccg cgcctggcca tgcggcgatc tccaccggtg ccacgccgcg cgtgcacgga
     6241 atcgtcggca acagctggtg gcgtcgtgac gaaggccgcg agcgaccggc cgagtacgat
     6301 ccggacgcgc ccgtattctc cgtcggtgac gcctacaacg gcgcgacgat ggatgcgggc
     6361 gtctcggcga agatgctgcg cgtcgacggc atcgccgatg cgctgcgcgc gcaaacgaac
     6421 ggcgccggca agtccgtcgc gatctcgttc aagcctcgct ccgccgtgct gatcgccgga
     6481 cgcaagccgg acctcgcgat ttggtacgac gcaggcgctg gcgggatgac gaccagcaag
     6541 gcctacgtcg atacggtgcc agcatggctt cagcagatgg cgaaggactc cccggtgaac
     6601 aagttcttca ccgcgacgtg ggacgcgcgc gaccccgtca tgtacggcga agttctcgac
     6661 gttcccgacg acgcaaaggg cgaagcatcg gagcacgggc tcggtgcgcg gtttccgcat
     6721 cagctcgcga cgtcggagca tcccgagcac gccatcgtcg agacaccgtt tgccgatcgc
     6781 gtcgtgtcgc ggacggtcgc cgttgcaatc gatgcgatgg agctcggccg cgacgaggtt
     6841 ccagacctac tcgcgatcag ctacagcgcg cacgactacg ccgggcacaa ctgggggccc
     6901 gactcgtggg aagtcctcga cgacacgatg cgcctcgacg ccgagctcgg cgagctgttc
     6961 gagtccctcg attcgcgcat cggcaaggac aactgggcgc tcgtcgtcac gagtgatcac
     7021 ggggcgacgc cggtcgtcga acgtgcgcgc attcgctcgg cgcgccggat cccgcccgtc
     7081 gaggtcgagc aagcggcgga caaggcgatc gcgcagctgc agggtggtgg accgtgggtc
     7141 gcgacgcaga cgtcgaacac gatctacatg acggcgaaat tcgcggcgct tcctgaagcg
     7201 cagaagaacg agtcgctcga tgccgcggtc gaagcgctca agcatctgcc tggcatcgcc
     7261 gtcgctggcc gcaccgatcg gttctcgaag gactgcaccg tcgagaaaga catttaccgc
     7321 gtgatgtgca acgcgtacgt gcccggcgag agcggcgagc tctacgccgt tgcgacgacg
     7381 ggctcgctca tcagcgagta caaggcgggc accggccatg acgcgcccta cgatgacaac
     7441 cgccacgtgc ccatcctcat caaggcgccc ggcgtgaagg cgcagcaagg gcagggcacg
     7501 ctgctgcagg tcgcaccgac cgtcgccgcg ttgctcggca tcgatccgcc gagcgccgcg
     7561 agagcgccga cgctgttcaa gatccaaaag cgctagcttc cgccgcgcgc gagcgccttc
     7621 gcgaagaacg gcgagtgctg cgcgagctcg gccatgtagt cgcgcacgtc ctcgcgagcg
     7681 gcggccggca attgtgcgaa cggcgtgacg tgctcgtccg gcacgtagcg aaatgtgaag
     7741 ttcacgcggc gcgtgcgaaa gcctgcgatc ttgggcggca gatcgacttc gcgcttgttc
     7801 tcgacgcgct gcacgcgatg caggagatcg tctttccagc gcgggcccgc gaacagctgc
     7861 agcgaattat cgtcgagcca ttcggtgcgc accggtggcg tgctctccgt cgcaccacgc
     7921 cggacgaact gaaagcgcgc gcgctcgccg agggatagcg atgcaaccgg tccaggctcg
     7981 aagtcgcggt gctcgcctac gcgcgcggca tccgaccact tgccgtcttg ctcgcggctg
     8041 ccgtagaagt tcacgagaca cgtgttgagg tgccactgcc gcggcacggt ctgttgcggg
     8101 aatccgtggc gaacccgttt ctcgatcgcc gcgacgaggc gcgcgagcac cggcggaaat
     8161 ggctcggccg cgacggccac gtgctttgtc cgtttcggcg gctcgtagta accgaggcac
     8221 gcgaactgcc agttgccgag ccagtacacc gggcggagca ggccgcgctg cgtcttgccc
     8281 gcgggcggcg ggcgcttcgt cgagtagcgc tgttcccaga gtgggtgcaa cgtcgcgagc
     8341 caggccagga tctccgcgcg ggacgcagga tcgatgaagt ccggctggta gacgtaacag
     8401 cggtcgaccg ggggcatcgg cgcgccacta tagcaaccac tcccgggcgt ggccgatagc
     8461 gtgcccatgc tccgctggct ctatcgtcct cagtgcatcg cctgcgcgaa accctcatcg
     8521 agcgagctgt gcgccgattg cgcgatctcg ctggtgccgc tcacgtcaca ggccgccgcg
     8581 cctgcaccgc ttctggggat cgcatcggca tgggctttcg gtggcgcact tgcctcggcg
     8641 attcgcaggc tcaagttcgc cggcgctacg cacatcgctc gcacgctcgc gccgctgtgg
     8701 gcgccggtca tcgaggccgc cgtcatcgag catgatgcga tcgtcgtccc ggtgccgctg
     8761 cattggcgac gtcgcgcgag gcgcggattc gatcagagct ggctgcttgc cgcgcatgcc
     8821 tgcgcgtttg cgaagcttcc gccgcccgtc gcggcactcc gccgcattcg ccacgcgaag
     8881 gcgcagagca cgctgcctgc agccgagcgc agcgagaacg tcgctggagc gtttcgcctg
     8941 cgccgcgaca tcgccggccg cgcgatcgtg ctcgtcgacg atgtcagcac gacaggcgcg
     9001 acgctcgctg ctgccgcacg gccgctgatc gacggcggtg catccctcgt cgtcggcgtg
     9061 accgtcgctc gcgcggagtg actactttcg ctcgaggacg atcagctcga accgatcgtt
     9121 gatctctcgc gcgcccttgc tcttcggatc gacgagcggt ttgctgctac cgaagcccga
     9181 gctctgcaga cgcagcggat cgatgcccca atcgacgagc cactcgcgca cggccttcgc
     9241 gcgcgcttcc gacagcgcca ggtcgttctt cgggttgccc gtgggctgga cgtgcgcggt
     9301 caggcgaatg cgaagcagct cgggatgcgc gcggagcgtc gcgccgatct gcgacaggat
     9361 gttcttgcta ccgcgcgcga tcttcgcgcc gccctcgaac aggatgttgt cgaggagatc
     9421 gaggcggtcc gggctcagca cgacgagcgc gtcgccacga tccgggcagc cgtcgtcgtc
     9481 cgcgttgccg ttgatcgact cgggctcgtt cggacacttg tcggccttgt cgacgagccc
     9541 gtcgttatcg ttgtcggcct cggcgcagcc atcggtgtcc tggaagccgt ctttgtcttc
     9601 ggcggcgacc ggacacttgt cctgatcatc ggccacgccg tcgttgtcgt tgtcacgctc
     9661 tggacaaccg tcctcgtctt cgaagcgatc gcgatcctcc gcgtcgaggg cgcacttgtc
     9721 cttctcgtcg gagatgccgt cgttgtcgtt gtcgacatcg gggcaaccat cgccatcctc
     9781 gaacagatcc ttgtcctcgg cctcgttcgg gcaggcgtcg agcttgtcct tcacgccgtc
     9841 gccgtctttg tcgttcggcc cctcgatgtg gatcttcttc ggcagtgcga caaccgcatc
     9901 agcgccgggc gtgaacgaca acatcaatgc accgcgcgcg ttcgacgtgc cgacacccga
     9961 ggtgatgcct cggccgagcg cgacgccgag cgtcgccgtg cgcgacatcc gatacgtgag
    10021 ctccgcgagg ccttcgatcg agctcgagag ctcgggcggg ccgtacatct ccatgccctc
    10081 cggatcgccg gtcttgccgc cgggaatcag gttgccgaag acctcgccgc cgatccacac
    10141 cttgttcgcg gcgcgccacg tgccaccgac ggcgaacgtt ccgccgctgc gctgcacgag
    10201 gctcgagtag cgatctttgc tgcgaatgat cgcgcctgca ttcatctgca ggacgaggcg
    10261 gcctttggtc agcgtcgcga ggccgaggag ccgagcttgc ggcatgttcg tgccggcaaa
    10321 ctcgctgccg ctgcgcgtcg gaatcgacag tgcgaacgcg ccgccatagc tgaggtcgcc
    10381 accgagtgct tcgagcgcgc cgccgttcat cttcgcgtgc accgtcgcgt cgccgcgcgt
    10441 gacgctatcg acctgcacga cctggaacat cttgttctgc tcgccggtcg cctgaccgtt
    10501 ttgcaacagc agcggcatgc gcaggcctgc ttcgaagcgc gtgccgaatg cgtacgcccc
    10561 gccgagcacg agcacgcttc gattgtcgac cgcggtgtac gtgttctgcg acgtcccgag
    10621 cacgagcggg ttgtccgcgt aggtgagcaa cgcgctgatc gcgtagtcac catccttgcc
    10681 gacgtcggct gactgcgatt gcagcgcggt cacctgcgtc gccggcgtcg gatcgaacag
    10741 cgtcgtatcg agattgcgcg tctcggcgtg cgccgcgctc gctgcaccga cgacaccaat
    10801 gaggccgagc aggccacgtg cctttcggcg tcggcccacg agcaatgcac cgagcacgag
    10861 cagcagcgca ccaccaccgc tgttgccgcc cgtcgagcag ccaccgccgc cgcgcacgtc
    10921 gacatagaga ccgcggccgc ggaggcgcac cgtcgagtgc gacgtatctg cgttgtcgag
    10981 gaagaggttc acgtcagcga cgaagtcacc ctcgtgcgtc ggcgtgaaca cgacgtcgaa
    11041 cttgtgcgtg ctctgcggcg tcagttgcat cgtctcggtg tcaccgatct cgtgatcgtc
    11101 ggtcaggtcg agcacgcgga acgcgccgtc gccgccggtg atcgtcatcg cgctcagctc
    11161 gaactgctcg gtcatttcgt gattggtgac gtcgagcgag agcactggcg tcgtcggcgt
    11221 cgcctcgagc gtcacgcccg cttcggatag gcgggtcgag atcccgatgc caacgtagcc
    11281 gaggttgatc tcgttgggcc cgaactcgac gaggcgatcg agcgcgatgc cgtcgagatc
    11341 gacggagacc accggcttgc cgttgtcgtt gttcatgatc accatgtggc ccgtcggagc
    11401 cttgcccgcg gtgacgggct cgaacttcac caagaactcg tagctctgac gaccggacac
    11461 atcgacggga tcgggattcg tgatcgtcca gatcggcgcg tcggaaagca tcacggccga
    11521 gacgttcaga ttcgcgtcgc cgttgttcgt gatcgtgacc ggcaggatgg gcgccttgtc
    11581 gccggggttc ttgaacgtgt tcgggaacac gggcgcaggc gccacgccga tatgacgatc
    11641 gacgccgtat cccgagatca tgaccgtcgc cgagcccggc gtgccgatcg cgcccgtcag
    11701 cggcacgacc acggtcgcgg tctccggctg attcggcgcg cgctcggccg tcggcgtata
    11761 cgtgacatcg aacgagtacg taccgtttgg cccgacggcc gccgtgggcg tcgccgtgaa
    11821 cgtgaacgga cccgtcgtgg tcttcgagac cgctccgatg ttcatcatcg ccgcgcccgt
    11881 gttcttgagc gtgaccgtct tcgtcttcgt cgtcggggtg atgtcgagat cgacagggcc
    11941 gaagtcgacg acgttaccgg gcgcgaggtc gaggaccgcg gtcgtcgagt tgcacttcag
    12001 agcgacgacg cggtcgggat tgagcgggag gctcgtgtgt accgtcagcg tgccaccgac
    12061 catgccgatg cgattgtttg gcttgcactg aacggtgacg accgtggtgt cattcgcacc
    12121 tgacgatggc gcgtacgtgg ttccagccgg acagccattc gtcgtggcag cagccgcgac
    12181 gccgttgtga cggcattgga tgatcgcgat ctcgctgctc gccgtgttca tgtccggcgt
    12241 gatcgacagc gacatgatgt tgtaggcggt acccgccgtg ttcacgatgt tcagcgtcgt
    12301 gttcgtcggc gtgtcgaatc ggatgtcacc ccagtcgact gggtatggat tcgacggaga
    12361 cgtgtcgtag ccggtcgtca ggccatcgcc gttgagactg agcgtcgtcg acgtcggcac
    12421 gttgccagag ccccacacgc cggagaacgt gtacgtcagc gcgccgccgt cgttgccgtt
    12481 cagcgggtag aactgcaccg tgacggtgac gctcgcattc gcgccgagcg acgcgatcgg
    12541 gaacgtcggg ctcaggatcg agtagccggt gcccgtgccg gtcatcgcgt agttgatgtt
    12601 cgtcaccggg aggttgccgg tgttcgtgag cgtgaacgtc tgcggtgtca tcgagccttc
    12661 gagcttctgc atgaagtcga acgagctcgc ggtgatcgtc aggttgccct tctggccgtt
    12721 acacgacagc gcaatgtcct ggccggtgag cgtgttgttc gagtccgtat tgacgtggaa
    12781 cgtgccggcg cggctgccct gctgcgtcgg cttgcacgcg atcgtccacg tcgccgtcat
    12841 cgtcggcggg atcacgatcg agatcgtcgc gccggtcgtg ccggtctgga cgacgtagtc
    12901 ggtcgcgcct gcgctgagct gcgcttgcga gacggtgagg tccgacgtcc cgttgttcgt
    12961 gacggtcaac gtctgcgccg tcgcggtggc gccgacctcg atgttgccga aggccatcga
    13021 cgtcacgttg tacgcggcaa gcggcgcgat gccggtgccg ctgagcccga cgtcgatcgg
    13081 cccggccgtg tctggatcgt tcgtcggcga gacgcgcagc acggcgttct tcgcgccaac
    13141 gctggtcggc cggaactgga cggtcacgag gaccgacgac atcgcggaca cgttgcagcc
    13201 cgacgtgcag cccgcagcgc ccgtcaggat gaactgcgtc gggtcgacgc ccgcaagccc
    13261 gacgttgtac gtgagcgtcg cgttgccggt gttctggatc atcaccgtct gaccggtgct
    13321 cgtcgtgttg atcagttggt tgttgaacgc gagcggcgtc gtcgtgacga cgatgtctgc
    13381 acggcccgcg gtgcagctga ggctcgtcgt attcgccgag ttgtcgtcgg catcgctcgt
    13441 gaactgcaca gacgcggttt gcgtgccgct cgcacctgcc ggcggggcgc actggacacc
    13501 gacggttccc ggcgtgccga cgatcgccat cgccagcgtg cacgtcgtcg tcgagccgac
    13561 gcagcctgcc tggttgaact tgaaccagcc ggcggtgttg ttgacgagcg ccgcttgcgt
    13621 gacgcccatc gtcgctttgg tgcccgcacc gttgttgctg accgagatgt tctgcgtcgt
    13681 cgatgtggtg ccttgattga tcgtgttgaa gttgatcgcg gtcgtcacgc cgatgactgc
    13741 ggtgccaccg gtgccgctca cagacaccgt cttggtggcg gtcaccgggt ccgtgctgtc
    13801 gatgttgatc gtcgcgctgc tcgcaccggc cgcaatggga tcgaatgtga tgacccacgt
    13861 gaccggtgtg cccggattca ccgttgcggg aagcgcgggc gtcgagctca ccgcgtagtc
    13921 gcctgcgccc gcccccgtga gcgtgatcgc gttgatctga agcggctgtc cggcatcgcc
    13981 cgtgttcgag atctggaggt tgagcgtcgc cgtgtgcgtc gagagaggcg tcgcgtcgtt
    14041 gtagcgaacc gtgccgaaag cgagtgaagc gaccgtactg atcaccggtg caacaccagt
    14101 tgctgtgatc gtgaacgagc cgagcgatcc ggtcgtcgtg cccgccggat acacgttgac
    14161 ggtgcacgat cgaccgccgc gtgtcgtcgg cgtgtacgcg acatcgacga cgttgttctg
    14221 gttgttcttg agctggatgt tcgtcgacgc gccaccgatg cgagccgtga acgtgcccgt
    14281 gccggtaccc gtgcagccgg agatcacgag atccgcgttg tagttctgcg tgtcgctcgt
    14341 cagatagacg tcggaattcg tgcccgtcgc attcacgacg acgttgccaa ccgccgcggt
    14401 ctttccgccg ctgcagttcg tcgagttaca aactccaacg acgatcgcgc tcgcattgcg
    14461 cgttacaaaa aacagcagca cgaacgctgc gagctgtgcc caacgcctga tggtcattac
    14521 gactcctgca gctcgtccgg agaccctctc ccggcgacga tgccgcgcat ctcccatcgt
    14581 acatgctcgg ctatcgctcg ggtaagcggc ccgaggcaca gacgtctgtc gtacatcctg
    14641 cgactcgcta cgaccgagaa cgtctcgtat cgtggttcgc acatgacgga cgacggcagc
    14701 gcgccaacca ttccggatac acaggcgccc acgcacggct cgacgcccag catcacgccc
    14761 gcgcgttcgc ccgatccccg ctatggcctg cgaacgcttc tgggaaaggg cggcatggga
    14821 gaggtctggc tcgcgcacga ttcgcgcatc gatcgagaga tcgcgatcaa gctcatgcgc
    14881 ggctcgagcg atccagattc ggcagcgcgc tttctccgcg aggcgcgcgt gcaaggacgg
    14941 ctcgagcatc cgagcgtcgt gcccgttcac gatctcggta cggagcccgg ctcgccgttc
    15001 ttcgcgatga agcgactcac cggcacgacc ctcgccgatg tcctcgcgga gcgcgaccac
    15061 accagcacca tccctcgccg cacgctgctc cagaagttcg tcgacatctg cctcgccgtc
    15121 gagttcgcgc atcagcgcgg cgtgatccat cgcgatctaa agccggcgaa catcatgctc
    15181 ggcgatttcg gcgaagccta cgtgctcgat tggggcctcg cgcgcatggc gggagacagc
    15241 agcgagagca tcgtccggcc aagcgatcgc gctggcgatt ccggaggcca gaccgaagtg
    15301 ggcgcgatgc tcggcacgcc gggctacatg gcgccggagc agatgcgcgg cgagcagatc
    15361 gatccgcgag ccgatgtgtt cgcgctgggc tgcatcttgt tcgagatcct cgccgggcaa
    15421 gccgcaatcc ctcgcgcgca tccctacgag gtgacgctcg cgtcgccgca ctatcggcct
    15481 tccgagcgcg cggccgatgt cccgccggag ctcgatgccg ccgtcgtgcg agccaccgcc
    15541 gctttgccgg ccgaccggca cgacaccgcg cgcgcgctcg ccgatgaggt tcagcgattc
    15601 ctcgacggag atcgcgatct cgagcgccgt cgccagctca gcatgcagca caccgaacgc
    15661 gcgcggcagc tcgcagggaa cacggacatc gagtcgcgcg ccgaagcgat gcgcgaagcg
    15721 ggaagtgcga tcgctctcga tgccggcaat cgcgaagcgc atgcagtgct gatgcagctc
    15781 ttgctcgagc ggccgaagga gctgcccgaa accgtgaaga agaagatcga tgaagatcgg
    15841 caacgcgcat ttcagccggt catccgcggc atggtgttcg tctacgcgtt cgcgctcgtc
    15901 accgcgctcg tcgtccggca gtggagcgcg cccggcgatt ggagactcgc gctactcgcg
    15961 ctcgagaatg gtgtgctcgc aatcctgcta tccgtcgcgt ggtggcgtcg ctggtacatc
    16021 acgccgtcgc tgttcacgat cggggcgctg ctccacgcgg cgatcctcac gacggtaggc
    16081 ttcgccgtcg gtccgctcct gctgttgccc gtattgctct tcggctcgtt gccggtcctg
    16141 atggtcgcgc cgatcattcg cgttccgatc gcggtattcg cggcgcacat cgttgcgttc
    16201 gcagttccgc tcgcgctcga gtggctgaac gtcgtgccgt cgtcgtatcg actggacggt
    16261 aacttgctcg tgctcgaacc gggcgtgggt ctaccggcga gcgtgacggt cgcgggcgtg
    16321 atcacgatcg tcctcttgca gctcgtcggc ctcagcacgg ttctcgatgc gcaacgccgc
    16381 gagcaagaca gcgcgcagga gaccgtgcag ctgcagtcct ggcagttcgc tcaactcgtg
    16441 cgcagctccg gatcctcgac gtgaacgaac ggagtatggt ccgcgcatgg agaagacatc
    16501 gagcggtttg gaatacgaag acactcaggt cggcacggga ccgagcccgc aaaagggcca
    16561 gacgtgcgtc atgcactaca ccggctggtt gtgggtcaac ggcgcgaagg gatcgaagtt
    16621 cgatagctcc gtcgaccgcg gcagcccgtt cgagtttccg ctcggcatgg gccgcgtcat
    16681 caaaggctgg gacgaaggcg tcgcgtcgat gaaggtcggc ggcaagcgca cgctgcggat
    16741 cccgccggac ctcggttacg gtcagcgcgg cgcaggcggc gtgattccgc cgggcgcaac
    16801 gctcgtgttc gatgtcgagc tgctcggcgt tcgctgatcg cacgcagcca tcgcggtcgt
    16861 ggtgctcgcc gccatcgcgc agtcgtacgt gccgcgcggc aaggtcagac acagctcgac
    16921 cgtcgagcgc agatcgagct tgtcgagatc ctcggcgctc ggctgctgat cctcggggcg
    16981 ctctttcacc gagagctgga cgacgtgctt cgcgagctgc tggcactcct ccgcgacgag
    17041 cgcttttgtc acaggcggtg tcgactcgat cctcgtcgga ggaggcggct cgggatgcgc
    17101 ggtcacgggg cccgcctgcg ttccgccgcc acacgctgcg agagcgagca ccagaagtat
    17161 gcgcgtcacg ctctaaatgt tagcgtggga ctcgcgatgc cgctctggtt tgccgcgctg
    17221 ctcggtctcg ttcagggctt gaccgagttc atcccgatct cgtcgactgc gcacctccgc
    17281 gtcgttccag cactcttggg tcaacccgac ccgggcgctg cgtacacggc ggtgattcag
    17341 ctcggcacgc tcgtcgcggt gctcgcgtac ttcgcgaagg acctggctca tctccccgcc
    17401 gcgatgatgc gcgagtctgg gacgttcgaa gcgcgactgc cgtggctgct cgccgtgggc
    17461 accatcccga tcgtgatcgc agggctcgtc ttgaagaagc acgtcgaggg agacctgcgc
    17521 tcgctctggg tcatcggtgg cgccttactc acgatcggta tcgtcttggc gctcgtcgat
    17581 cgtcatgtcg ccggccgtcc gtcgtttcgc ccgctcgccg atctgacgta cgtcgatgca
    17641 ctgatcgtcg gtctcgcgca gacgatggcg ctcgttcccg gcgtctcgcg ctccggctcg
    17701 acgatgtgcg cgatcctgct gctcggcttc tcgcgctcgg actcggcgcg gttctcgttc
    17761 ctgctgtcga tcccggcgat cgcgggcgca ggcatcttcg aagcgcgtga tgcctttcgc
    17821 acgctcggct cgagcgcggt gcctgccatc gcggtgggca tgatcgtcgc tgccgtcacg
    17881 ggctacgtcg caattgcgtg gttcatcaag ttcctcggcc aaaactcgat gatgggcttc
    17941 gccgtctacc ggatcgcgtg cggggcagca cttctgaccg cattggctgc aggttttctg
    18001 cgccgcgttt gagaaacatc cctacggctg atagcctcgt aggcgtccgt acttagaggc
    18061 acgccatgag gattactcgg ctcgttgtct tgtcgctcat caccctgggg atgctcgcga
    18121 ctgcggcgct atcagcgcca tgccccacaa ccacacctgg taagtacccg gtgaagatcg
    18181 attctgcgcc ccaaggagcg caggtctaca tcaatgacaa gacgtgcctc gtcggcaaca
    18241 cgccgtggag cggcaagctc gcgaagggca cgcacacggt catcatcgag gctaccggct
    18301 acgagccgat gtctcgccag ttcaacgtcg cagccgtgcg caagacgcag gagctgttcg
    18361 tcccgctcgt gaagaaagcg gacccgccga agatcgacgt caaagccgac gccgatccga
    18421 agggtgtcgc gggcgcgcaa gtctgggtcg acggcgagat gaaaggcgcc gcgccgatct
    18481 cgctgacggt caccgaaggc cgccacctct tgcagctgaa gaaggacggc tacgagacct
    18541 acgagacgtg ggtcccgacg tcggcgaaca acaccgcgac gctgcttccg cagatgaagg
    18601 aagtcccgaa gccgaagttc ggcaccgtcg tcgtcgaagc cgacgttccc gacgccgagg
    18661 tctacatcga cggcaacaag catcccgaca acacgcccgc gatgatcgcg aacgtgatcg
    18721 aaggtccgca catcatcgaa gtacgcaagc cgccgagcct gccgtggaag caaacggtgc
    18781 aggtcaccgc agacaagtcg acgaaggtcc gcgccgagct gcaagcgacg atgcacggcg
    18841 gcgtcggtgt cgtgcgcgtg ctctcggatg cgcagggtgc gcgcgcgttc atcgacggca
    18901 ccgacatggg accggtcccc gtcgacatca aggacgtcaa agcgggcgag cacatcatcc
    18961 aggtcaaagc gcctggcatg aagaccgacg agaagaaggt cacggtcgcc gccggtagct
    19021 cgcagatcgt gaagttcgat ctcaacgccg agacgcccgc cgatcaaggc acgctgaagg
    19081 tcgtctcgat ggttcccgaa gcgcaggtct tcatcgacgg cgcggcggtc ggcaaggttc
    19141 cggtcgacaa gaagctgtcg gccggcgagc acccagtcgt cgttcgcctc gagggcttca
    19201 agcagttcga gcaaaaagtg cgcgtcgagg cgggccagac ggtcaccgtg caagcgaccc
    19261 tcaagccggt cggccgtctc cgcatcctgt cgacaccggc gcaggccgag gtcgtcatca
    19321 acggcgttcc ggccggcaag acgccgctcg atcaagaggt cgagacgggc gagaccgtca
    19381 ttcgcatcga gctggccggc ttccagccgt tcgagcaaac gctgatgatc gaaggcggca
    19441 agacgcagac cctctcgcgc gagctcgcga tcgcaggtct ctccgaggcg gagctcgctt
    19501 cgcagcagcg cggtctgtcg tcgttcggtg cacgcacgct gccgcgcggc cgctcgacgg
    19561 tcgacttcag cggcggctat ccgtactacc tcacggcgcg catcaacgtc ggcgccggca
    19621 agatcgccaa gaagttcggc ttcgacgcag gcgtcggtgt ccgcacgatg ttcgcgcgca
    19681 acgagcttgg cctcggtggc cgcatgatgc tcgccgatgc cgatccgttc tcggccggcg
    19741 tgttcacgga catctggtgg gggagcaagc tcctcgacga ttcgcgccgc aacggcttca
    19801 cgtgggacgt cggcgcggcc gcgtcgctca cggcgctctc gcacgtcacg atcacgggcc
    19861 gcttctactt cgacatctgg agcgaccggc actgccccga gctgaagacg ggcggcaccg
    19921 gcggattcga cggcgatccg atcgcggtct gcgaggcgta cgccgcgaac acgctgaccg
    19981 tcgacgagac gatgcgcatc aacaagctga cgacgggcga cgaagccacc gcatggaagt
    20041 tcgccgaccg cgaaacgggc attcgtctga tggcgagcgt catcgccgag atcgccgtcg
    20101 agcagcgctg gaacctgttc ggcatcctcg agggtgcgcc attccagaac gagcgcgcgc
    20161 tcttcacgag cgacttctcg cactcgatgc cgtcggactc ggacttcaac ctgtacttgc
    20221 gcctcggcgc ttcgtacaag ttttaatcag gcggtcgtcg gtgcgaacgc gatcgcggag
    20281 tatgcgatcg ccgtcgactt gcccgggttg aagtacgcgt ggcgctggtc gccgcggaag
    20341 ctgacgacgt cgccttcgtc gagcgtgtag cgctgtcccg cgacggcaag ctcgacgctg
    20401 ccgcgctcgc acgtcaggta ctcgcgcgtg cccggcgtgt gcggcacacc cgccatccgc
    20461 gcgccgggcg gtagcgtcat gcgctccata tcgaggcccg gcagcggctc gggcaggagc
    20521 ttacgaatga gcacctgacc gcgatcacgc gtcggcagct ccgcggcttt caggaagcgc
    20581 gcgggctgtc tgggtgatgc aagcagctcg tcgaggcgca cctgcagcgc cgccgcgact
    20641 ttgatcaaca cggccagcgt ggggttcgct gcgcccgact cgagatgcgt ccacgtcgcg
    20701 cgggggatgc cggccgcctt cgccatctgc tgctgtgaca gcccgcgggt ctcgcgtaac
    20761 gccttgatgt tgtctgcgag gtggtcgtgc tctccgccca tgcagtggcc tcctgccaac
    20821 gtattagcac gcctgatgac atattggctg gcggtgtgta cccctgaata gcaggtagcc
    20881 gaacgaacag gaggagacac agatgaagat ccaaggacag ggcgcactca tcaccggagc
    20941 aagtcgcggt ctcggccgcg cgctcgctga agcactcgca caacgcggcg cgaaggtcgc
    21001 gctcgtcgct cgcgatatcc gcccgctgca agacgtcgtc gcaaccatcc gcgcgcaagg
    21061 cggcgacgcc cacgctatcg ccggcgacat cgcggccaaa gacgcggtgc atcgcatcgc
    21121 cggtcaagcg ggtggcctcg tcggcgagat cggcatcgcg atccacaatg cgagctcgct
    21181 cggcccgacg ccgctgcgcc tcctcctcga caccgaatgc gaagacctcg ccgccgtcct
    21241 cgagacgaac ctcgtcggtc cattccgcct gacgaagatc ctcgccggcg cgatggcgct
    21301 gcgcggcgcc ggcactgtcg tccacatctc gtcggacgcc gctgtcgagc cgtacgcacg
    21361 gtggggcgcc tacgcgatct cgaaggccgc gcaagatcac ctcagccgca tcctcgccgc
    21421 cgagctcgca gacacgggcg tccgcatcct cgcgatcgat ccaggcgaga tggacaccaa
    21481 gatgcacgcc gacgcgatcc ccgacgcaga ccgcgcaacg ctgcagcgcc ccgccgacgt
    21541 cgccacgcgc atcgtccaaa tgatcgaaga cgaagtgcgc gcgcccaccg gctcccgcct
    21601 caccgcgccc acgtgggcgg ctgcgccgac cgcactgacg tccgtgccgc cgcccggagc
    21661 gacgcccgcg tcgacgacag aggcaaagcc atgagcgccg cgcctttccg tcgctacgcg
    21721 cacaccgtgc cgagtgagct gaagctggcc acgaccgctc catccggcga acgggagagt
    21781 ctcgtccgag gcgcggacac tgtccgtgcg tcggacgcgg acggccggcg ctcggacgat
    21841 cgccaggact cggacgcgga tgcccgacgt gcggacgcgg aggatcgccg ctcggacgcg
    21901 gatggccggc gctcggacgc ggatggccgg cgctcggacg ccgatggccg gcgcgcaggc
    21961 gcgagcgagc tagaacctac gcgtgcgttg cgcacggacg aggtctctcg tcacgctgtt
    22021 cggctcgtga cggtggcgcg cgccgacgag cgcgttgcga tcacgtcgtt cgcggagctg
    22081 ccgagcgtat tgcgcgcggg cgatctcgtc gtcgtcaacg atgccgccac gttgccgggc
    22141 tcgctgcgcg gccagaccga gcgcggcgag gcgatcgagc tgcggctatc ggggcccgtc
    22201 gagcgcaatc ggctcgtcgg cgtgctcttc ggcgccggcg atcatcggac gcgcaccgag
    22261 caccgcgctg caccgccgac gatcgcggtg ggcgaggtcg tgcaattcgg cgtgggcgat
    22321 catcggacgc gcaccgagca cggcactgca ctaccgacga tcgcggtggg cgaggtcccg
    22381 cgattcgcgg cgggagatgc gccgtaccgc gcgctacacg cgcgggtcgt ggcgacgagc
    22441 gggcggcgcg tcgagctcga gacgcgcgag agcggcgatg agctgtggca ggccttgtat
    22501 gcgagcggtg cgcccgtgca gtacgcgcat cggccgaagc tgctgccgct gtatgcggtg
    22561 cagacggcgt atgcggcgag gccgtgggcc gcggagatgc cgtcggcggg gcggccgctg
    22621 acgtgggatg tgctcatcgg gct
//
LOCUS       DATLBZ010000273        22491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9841439, whole genome shotgun sequence.
ACCESSION   DATLBZ010000273 DATLBZ010000000
VERSION     DATLBZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22491
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9841439"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VMZ53_14370"
     CDS             <1..262
                     /locus_tag="VMZ53_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS29684.1"
                     /translation="LQCEQFAGRIHLMVTDVVMPRMSGRQLADRLEVQRPDMNVLFVS
                     GYTEDAIVRHGVLNAGLEFLSKPITPDTLARKVREVLDTPRS"
     gene            271..909
                     /locus_tag="VMZ53_14375"
     CDS             271..909
                     /locus_tag="VMZ53_14375"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HUS29685.1"
                     /translation="MKILYLHGFASGPLSKKGVEFATYFTERGHQVERLNLRVPSFEH
                     LRLSAMLDTVRDAIDRVGAPVVLIGSSLGGLTAARVAERDERVAACVLLAPAFQLVER
                     WQQQLGAEWDTWRERGTREVADYTTGGMSSVDFGFVEDVARIDVGYPDVRVPALVMHG
                     IHDEVVPVDRARTFAAGRANVRLLELDDGHELVASIPRLLAESDAFLAGLAL"
     gene            925..1806
                     /locus_tag="VMZ53_14380"
     CDS             925..1806
                     /locus_tag="VMZ53_14380"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HUS29686.1"
                     /translation="MMEALARARLALDGLSVADAFGEQLLHAGPAARSIALEHRSAPI
                     GRKWMWTDDTAMALSIIEQLAERGGIEPHALAAAFARRYIAEPMRGYGGGAHKVLGEI
                     AAGEAWDVAARRLFDGQGSCGNGGGMRSAPIGAFFCDDLAAVVDHAARSAAPTHAHPD
                     GEAGAVAVAVAAARVFAGERDPRALLEAVVEHTPAGATRDGIRRSIPMLKAEHYTVAA
                     ELGNGSRVLSADTVPFAIWCAATHLEDFVGACWSCVEVGGDIDTTCAMAGGIIVGATG
                     LAGIPDGWLSSREPLPV"
     gene            complement(1810..2655)
                     /locus_tag="VMZ53_14385"
     CDS             complement(1810..2655)
                     /locus_tag="VMZ53_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29687.1"
                     /translation="MKHVIALVCLVGCNQFFDLEKTESFDSGGDLDRDGVTDDRDNCP
                     SLANDQANADGDAFGDACDTCPQLASTSTHDEDRDHVGDDCDACPGVGDFGEDPDGDG
                     IGAVCEVRPAIADVRVAFEPFDDMPAGWQADSVAWTQSDDAIAPDADLPMSDRGLQNL
                     SLATPMGVWVAGIGVTSRSRWMAADRYGIVAHRGTRTFACEMACSSAMCVGSTKVDGA
                     TASSFKLLPQPYTRVRMVASNGQALCHFEGSGSAPASAFPTDGATFALIASPRIRVAY
                     FEYIE"
     gene            complement(2652..3521)
                     /locus_tag="VMZ53_14390"
     CDS             complement(2652..3521)
                     /locus_tag="VMZ53_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29688.1"
                     /translation="MRWAGIGVYALVAVSAAGCNAVFDLAPTIPLDAATDFDHDGVDD
                     GRDNCALLANADQANRDEDAFGDACDTCPQLASASVHDEEGDFTGDDCDPCPGIPDFN
                     DDTDGDGIGDLCDPEPGPPNRRLLFETFEAIAPEWHATGVPWTGGQDAVWPTTNLPST
                     DRGLENSSLIVTNGYTVVVGVESSMRWTTGDRVEITATRSADAYGCKLECTASTCNGS
                     LTFGSSGGSGVGIVGARPYMRAVMARSTAGYLNCSWGEAGGIGTPSTTASPTYFSVAG
                     SAQVHVAYVEVIE"
     gene            3557..4192
                     /locus_tag="VMZ53_14395"
     CDS             3557..4192
                     /locus_tag="VMZ53_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HUS29689.1"
                     /translation="MSHEDLKFALVAFSAIFFVIDPLANVPVFLTITATDTPDQRRRT
                     AARGALAAWVTLTIFAIAGGLIFQAFGISLAAFKIAGGIMLLLMAIDMMRAQPSPTRT
                     TAEEQGEAQARDDVAIFPLAIPMLAGPGAIATVMVLMSRAAWDPVRTPLVFVAVSVTC
                     IVCWLTMRSAANAEKWIPKTLLRAFERIMGLLLAAVAVEFIAGGVKEIIGK"
     gene            4192..4512
                     /locus_tag="VMZ53_14400"
     CDS             4192..4512
                     /locus_tag="VMZ53_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29690.1"
                     /translation="MNRALLTLVLVGCSNPPPPKPPAPPPSQCARVADHLLELMTPTA
                     QDAPTDQLDTMRRTFNDHCKNDGWSSRAQDCMLAASSLQQVGERCQLLLTPEQNAALV
                     PPAS"
     gene            4670..6205
                     /locus_tag="VMZ53_14405"
     CDS             4670..6205
                     /locus_tag="VMZ53_14405"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS29691.1"
                     /translation="MGDAALKVQTRFATAVELLKAVGTALERGSLRLPLPLKFETPFK
                     VTLVAANGAEAVRGTAEIVEHAGHATWIRFLSAGPELPGDDASRCMLADVDVDVTGPQ
                     EAVVEPAASSTSEAFDAITAVNDRPPALASMMGTAEVVPLVAAPPEPQPTVAATEAPS
                     AELPGSIADAVRSVEASEASTAPIPVETMAEVVASSREVVAVVDDRSDVSSSTGAAVS
                     VVTEDAEAIYVNEKRDTEASPPLVVGEHLKDSVAAERGTEPIAAHTIDAVAAERGIEP
                     IAEPYVRESLPVVPQAMPTARRIVIASVSFAAVCMAVSMVALVWAHDAVAEAETKPTP
                     ASTPVVEADPVEAVADVEAAPVVEAAPAVEQVADEPTPSTCTLEIDASVAESRVKIDG
                     KDRGLSPTTISVPCGQPVDVEVRHARYATYERTITVTGVREALRAKLERDKTTVTLTS
                     DPPATVTLDGHVIGKTPMTTTVSRFEQSTFRFSAPGYESDWRRILPKTGASSVVLTLK
                     PQR"
     gene            6253..8412
                     /locus_tag="VMZ53_14410"
     CDS             6253..8412
                     /locus_tag="VMZ53_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HUS29692.1"
                     /translation="MSLPARQLFSYRPYWAKRFGVAPFLPTTRAEMDELGWDSCDVIL
                     VTGDAYIDHPSFGMALVGRLLESHGFRVGILDQPEWRTVDAFRSLGKPNLFWGVTAGN
                     MDSMVNRYTSDKKVRSDDAYSPDGQAGLRPDRSVLVYSQRCREAFGDVPLVIGGIEAS
                     LRRIAHYDYWSDKVRRSVLAETRADLLVYGNAERAIVEIAHRIAARTPVTEIRDIRGT
                     AFIGKRDEFSEVDSTSIDAPGRVEPKPDPYAMEAERQEAPCATGDAAAAPADNLIQLR
                     PRKVDREKQAIRMPSFEQVTADPVLYAHASRILHLESNPGNARALVQRHGNHDVWINP
                     PPLPLTTQEMDALYELPFTRLPHPKHQPTKLPAYEMIKFSVTILRGCFGGCSFCSITE
                     HEGRVIQSRSEKSILAEIERIRDQTPGFTGIISDLGGPTANMYRMACKTREVESACRK
                     PSCVFPDICDNLNTDHSSLVGLYRKARAIKGVKRIFIASGVRYDLAVRSPEWIRELAQ
                     HHTGGYLKIAPEHTEDGPLRHMMKPGIGAYDRFKELFDRYTAEAGKEQYLIPYFIAAH
                     PGTTDEDMVNLALWLKRNGFRPDQVQAFLPSPMASATAMYHSGFNPLRSLKAKERVGT
                     VKGLDRRRLHKAFLRYHDPNNWPMLREALERMGRSDLIGSGKDKLVPAWQPRGTGSAH
                     EGRRGSKGIRPGTALTQHTGLPPQPHKNRSRKPRSSR"
     gene            8480..10042
                     /locus_tag="VMZ53_14415"
     CDS             8480..10042
                     /locus_tag="VMZ53_14415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29693.1"
                     /translation="MGTPSPDDSGVVALGSVLDGRYRVEAILGKGGMGRVYKAEHTSL
                     GRAVAIKVLHARLGGSKEAAQRFQREAMASGRLDHPNIVSVSDFGTLEDGSLFLVMEA
                     LEGEPLGNRIERDKRIPWQESLVIIRGVLAGLRHAHDKGVVHRDVKPDNIFLANKDGE
                     PVVKILDFGIAKLYAGNADDPASTRAGLTVGTPAYLSPEQAVGGAITPASDLYSTSVV
                     LYEMLTGRPPFLDDEPLAMLTAHVSREPPPFSEVAPDLQIPPGLEAVIQHGLAKVSAE
                     RISSANEYLGRLDEVTRAAGYDLGMMLPRSSGSLAIPAGPHAVTPSPGFLMTPAPFPA
                     HPGNTPAAGVHVMTPPGGVHAMTPMPFSSEMGTAPTQSLDSMPQAARMVSVADIASKE
                     PLPSKYKKIGIAMIVMAIALSIFAVVRSSDKAGATRGTTGPIPGGPPIDNETRLKAAL
                     HDLETGKTCADRKAAIPTLVDIGDERAIPSLKKARYRMRGGVIGIGDSNTNACLKQDA
                     EMAIQAIAPQSD"
     gene            complement(10043..10921)
                     /locus_tag="VMZ53_14420"
     CDS             complement(10043..10921)
                     /locus_tag="VMZ53_14420"
                     /inference="COORDINATES: protein motif:HMM:NF025391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HUS29694.1"
                     /translation="MAKTSKSQKGKAAAQKALKTVKAAGKAAKAAGKAAKAAVVNVVK
                     PTILKAKETVDKKTGRAVVRAIKPAKATKPSMPAPPPPPKVEEPAQRTELAAPRDIAR
                     LTRYGERFGANKIDVRWLPIQLPVTSGSLAVMDPGVPKSFKVIDRPVGNGQFRAMLSI
                     ARTDDGKERIAAITIHVGRPPITRWTVAHWRGQKPPKSADQLPRTEVSTGWLALLDAG
                     KGEKDSPGMLAVAAANGLNPVEVPLTDGRRALALPCGNGEFVAYWAVDAADKPICLVI
                     DFEAFSQKDWKARPPA"
     gene            10982..11959
                     /locus_tag="VMZ53_14425"
     CDS             10982..11959
                     /locus_tag="VMZ53_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29695.1"
                     /translation="MRQVTWTLATLVGLGGVAAANPGESPGVDSSTVTLGVESRSQRG
                     VAQDYLVLPSGGELGAQMRFITAPRGPIFEGEGEQKLEFTDLALFGVSARWSLFSKLE
                     LSASATLLPKQPAFTDEKPWQSVGVGLRSPLGRNAAVAVSGGGGHLMSHAGMWTRESL
                     MLEWKKPIHEDFLSFDIQGGIQGLGIQAPKTTKSTAMITEVAIQTTALFREPHGKWGA
                     WLGMGYAVPVQFSGKDPTTEMWINPQPRLDFHMGTVLALVPKWDLFVDFAVVDRGELS
                     DPTTRLPILDGGFDQRQVIFGVTRHIEGSKARSYDDYGDDDGAIRVGTL"
     gene            12101..12814
                     /locus_tag="VMZ53_14430"
     CDS             12101..12814
                     /locus_tag="VMZ53_14430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS29696.1"
                     /translation="MQAFGSKTSPAARNRDQLIREHADVARRIALRMARRCPEWISRE
                     DLVSAGMVGLIEAADRYDESRQEPFIAFAEHRIRGAILDELRRGDIMPRRVRQLARKI
                     SAAIKELEKDGTQASDQRVADALGVPVEQYRTGLAQLVHVEMQPLDGEETRLVAQDVL
                     PDMQADHRQMLARIRSSLDRMEARDVTILGLHYVEDLTYQEIADTLKITPSRVCQLLW
                     RAVERLRTQLGAEVMAAAA"
     gene            12811..13197
                     /locus_tag="VMZ53_14435"
     CDS             12811..13197
                     /locus_tag="VMZ53_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS29697.1"
                     /translation="MSTRVLVVDDDPLVCRAISVVLARGGFDVQNANDAAPALQIAAQ
                     WTPDIAVVDYNMPTCGLALVRELKARLGDNVFVAVLTGDDDDEMREKCHAGGADAVLI
                     KPIAPVELRRRLTAAAVALKTLAAAS"
     gene            13272..14648
                     /locus_tag="VMZ53_14440"
     CDS             13272..14648
                     /locus_tag="VMZ53_14440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS29698.1"
                     /translation="MVLEDDEDVSRAIARKLRADNFAIEESMDPVPVLQRLEQGNADW
                     DVVILDMGLPGMTGLEVLHRFREAGSFAACIMLTGDRSAGTATECMRAGAFYYLTKPF
                     EPFQLSSMVQSAARYSQMRRQLATRETKEDVSLVGASAAMRKLRASLDRLGDQDVPIL
                     IQGESGTGKELVARALHERGARRRKRFVALNCGAIPETLIDSELFGHIKGAFTGATTD
                     RAGVFVEADGGTLFLDEIGDMPLAVQARLLRVLQESEVRPLGGSGNRKVDVRVIAASH
                     VDLAQAVEQGRFRQDLFYRLNVVVLRVAPLRERLDDLPLLAAHFIRKHGGSKPPTLSP
                     DALEAMMDYPWPGNVREVENAVLHAIALHHGDAIGAESLPATISGRRASASSPSLQSV
                     DDDAANLEPLTEAKRRASSAFEKRYLTKAMERAKGSVSEAARLAGLDRTNFRRLLQRH
                     GIDPASFK"
     gene            14731..14991
                     /locus_tag="VMZ53_14445"
     CDS             14731..14991
                     /locus_tag="VMZ53_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29699.1"
                     /translation="MRDEILDEAKALEAFGVLTRSATHDLNNQMAAIMTFADLVLEAL
                     PESHPIRHEVQQILAAGGRAVAKTRELSMIARRLSPIGRRAS"
     gene            14997..15254
                     /locus_tag="VMZ53_14450"
     CDS             14997..15254
                     /locus_tag="VMZ53_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29700.1"
                     /translation="MSIVSAIEKLLLIAEYRDDVRQLSLDVVEISGQIQTILIVVESA
                     MRNVGHDAALRADLEEIRQAAVRAIMKVEPLSLGYGVLRAV"
     gene            complement(15202..16755)
                     /locus_tag="VMZ53_14455"
     CDS             complement(15202..16755)
                     /locus_tag="VMZ53_14455"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HUS29701.1"
                     /translation="MGLVAIALVLSSMHYEGDVTAAGGDYVDVPFDVPVGTVEIQITH
                     TDGSADVILDWGVWEPGGRFRGWGGGNTEDAIIGVDESSRSYLPGPIPAGTWTVSIGK
                     ALLDGAGTGHYSIDVTCSDTATLTPQARAAFAPVVLSTERRWYKGDFHVHSVQSGDAN
                     ASFAAIATLAKSRGLDFVNLSDHNTYSQHALIAAAQPSYPDFLFLRGAEITTYSGHGN
                     SVGTHDYVDHRLGYNGRTVQGIVDDVAAQEGVFIVNHPVLDLGTQCIGCSWKHVDDTP
                     WDKVSGMELITGNFEIGIQAFVPRVVTLWDQLEGADHHIAVVGGSDDHRAGTDTGPTA
                     SKIGSPTTLVLADNLSEAAIVKAIREGRTAVMLRNAEDPTIDLTITRADGTAAEIGDE
                     VQDLGEVHATVHVVGTDPDGAPLIVQLWRDGEKIDQKPVTAADVKIAFRDTPGVQLRR
                     YRAELINDLNQRLVVTSHIYVHGAAADEGCGCSTSTGRSDGLFVVGALLFVRRRAGRR
                     SRATAARPS"
     gene            complement(16768..17640)
                     /locus_tag="VMZ53_14460"
     CDS             complement(16768..17640)
                     /locus_tag="VMZ53_14460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS29702.1"
                     /translation="MAQDLVGKNFIVTGGNSGIGMVAARDLASRGARVIIASRSEAKT
                     RPVIDEIKKTTGNDAVEYAKLDLGDLDSVRACAKELLARDIPIHGLLNNAGFASGLRG
                     QRGVTKQGFELTFGTNHLGHYLLTRLLLDRIKQTPGARIVNVSSVSHYQVRNAFPWDK
                     LQKPTKSFAGLGDYAVSKLANVLFTAELAKKLEGTGVTTYAVHPGTVATDVWRGVPQP
                     LRWAMKKAMRMLTPEQGAVSMLRAATDPALANDTGKFYWLDGNERKPSRLARDADLAR
                     ELWNRSAEWVGLPA"
     gene            17667..18113
                     /locus_tag="VMZ53_14465"
     CDS             17667..18113
                     /locus_tag="VMZ53_14465"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HUS29703.1"
                     /translation="MTRWLLALVTVAACGGRTTTPAPPAKPAGKPLYDRMGGMDVIDA
                     VVKDFVEVRIAQDDRIRDRFKTADRAHLQMRLAEQICELSSGPCKYSGKHMRDAHAGI
                     GIGDAELRVFLDDLRRSLETLRVPAREQDELLTALGKLHDEIVQAQ"
     gene            complement(18107..18715)
                     /locus_tag="VMZ53_14470"
     CDS             complement(18107..18715)
                     /locus_tag="VMZ53_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HUS29704.1"
                     /translation="MKGLVVAALLLAACDGDIDSSMMTGDAAGSGSNHVYMDAPAGTG
                     EPMNLAGITDAHNMVRAAVDTTGIAAGPLPLMVWDPALATLANNWASQCIDADNNGFV
                     DHSSQAYRTNAAGYSYVGENIFASGGAATAQQAVATWASEKSKFTYPTGCSGTCGHYT
                     QIVWRTSIHIGCANVTCGSLQYKGNLVCNYGPGGNSSGGAPY"
     regulatory      18795..18923
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            18994..21090
                     /locus_tag="VMZ53_14475"
     CDS             18994..21090
                     /locus_tag="VMZ53_14475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS29705.1"
                     /translation="MNKTGSTLLLLALCARASAQDAPTGSAAQEVDPETIVIIDRAPD
                     PDAAPGARDRDRMLGDAPFVTIVHADDHPGTTSVADAVGATVGVQSRSLGGLGAYESI
                     SVRGAAPGHTVVLVDGIPLARLAAVTTDLGRYSLSSFSEVELYRGAVPVELGGAGVGG
                     ALNLVTRLGRGERGERITASLGAGSYGARHLRARYGDRHFGRIESASTLGYQAATGDY
                     TFFSDNGTLLNKNDDTYQVRKNNGFSQLDAATRLGNLDRSSVGGVRLAWKNQGLPGSA
                     TQPSLEAGMSTLDVIGDARLDLHAGSATARQLVYGLVEKQRLDDPAGELGLGSQERGY
                     LTLSGGASSTWQLPLGVHRATAGIELRADRFKDSDGTGMRQPFVGDREGGAALAAIDF
                     NLDPTIVVTPALRFDLVRTAPTPMGTGPTAGQPMPIRRDAVPSPRISARAAVTEDVSI
                     KTSAGWYVRLPTLVELFGNRGAILGSPDLLPERGPTADAGVVWAPAKARGDFDRILVE
                     ASVFGTRSHDTIAFINSSGFITRAVNLANTQTYGGELVASMRIGRALSVTANYTRLVT
                     QQLSEDVSFANKAVPREPGHAVYARADLAHRLFHRRASIWLDGSWQSSTYLDQANFMM
                     VPARLLAGTGARIELAGGLAASLAVENLADTRVQQLPLDPPPRPDLTATPTALADVAG
                     FPLPGRTVYVSLDWSY"
     gene            21091..22236
                     /locus_tag="VMZ53_14480"
     CDS             21091..22236
                     /locus_tag="VMZ53_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29706.1"
                     /translation="MRRSIVLSLALSVVLVACGDNKDVPDARMGIDAPPPDAHIVTPR
                     AVAVAGDFGSPGVGTVSKLEVTDLMMRQNVVQGAAQGDPALRVFGNRVYVVNRFGSNN
                     ITILDAKTLSVVDQMSTGASSNPQDVAVVDNKMYIPALGTAGVVVLAPGSPTPQVIDL
                     ATPLGDADGKPDCVSAFAVGKYVYVACDMQTNFANVLDSKIAVIDTANNHVVGQVITP
                     YKNPLGLFAHAPQAAVYFGDLLIPLVPNFGDLTQGCIARISTVNPAAPTAACGPTNAE
                     LGGFANRLAVDSENNKLYIAVAESFTSGKLRTVDLSNGALAAGSLSKAGQLIVDVQVC
                     PGGDVVASDQTTNATGLRVWRGTTERTTDAISIGMNPTVNGLACYDP"
BASE COUNT         3793 a         7477 c         7349 g         3872 t
ORIGIN      
        1 ccttcagtgc gagcagttcg cgggccggat ccacctgatg gtcacggacg tcgtgatgcc
       61 gcgaatgagc gggcggcagc ttgccgatcg tctcgaggtt caacgcccag acatgaatgt
      121 cttgttcgtc tccgggtaca ccgaggacgc gatcgtccgc cacggcgtgc tcaacgccgg
      181 actcgagttc ttgagcaagc cgatcacgcc ggacacgctc gcgcgcaagg tacgcgaagt
      241 cctcgataca ccgaggtcgt agcttatcgg atgaagattc tctatctcca cggcttcgcg
      301 tcgggaccgc tctcgaagaa gggcgtcgaa ttcgctacgt acttcacgga gcgcgggcat
      361 caggtcgagc gtctgaatct tcgtgtgccg tcgttcgaac atttgcgtct ctccgcgatg
      421 ctcgacaccg ttcgcgatgc aatcgatcgc gtgggcgcgc cggtcgtgct gatcggctcg
      481 agcctcggcg ggctcacggc tgcgcgagtt gccgaacgcg atgagcgcgt cgccgcatgc
      541 gtgttgctcg cgcccgcgtt ccagcttgtc gagcggtggc agcagcagct cggtgcggaa
      601 tgggacacgt ggcgggagcg cggcacgcgc gaggtcgcgg actacacgac cggtgggatg
      661 tcgtccgtgg actttggctt cgtcgaagac gtcgcgcgca tcgatgtcgg ctatccggat
      721 gtgcgcgtgc cggcgcttgt catgcacggc atccacgacg aggtcgtgcc cgtcgatcgc
      781 gcgcggacat ttgcggcggg gcgagccaac gtgcggctgc tcgagctcga cgacggtcac
      841 gagctcgtcg cctcgatccc gaggcttctc gcggagtcag acgcgttcct tgccggtctg
      901 gcgttatagt gtcaatctaa caccatgatg gaagcactgg cgcgcgcacg gcttgctctc
      961 gacggtctgt ccgtcgcgga tgcgttcggc gagcaactgc tccacgcggg acccgccgcg
     1021 cggtcgatcg cactcgagca tcggtctgca ccgatcggtc gcaagtggat gtggaccgat
     1081 gacaccgcga tggcgctctc gatcatcgag cagctcgccg agcgaggcgg gatcgagccc
     1141 cacgcgctcg cggccgcatt cgcgcgtcgc tacatcgcgg agccgatgcg tgggtacggc
     1201 ggcggcgcgc acaaggtgct cggcgagatc gctgcgggcg aagcgtggga cgtcgcggcg
     1261 cgccggttgt tcgatggtca aggctcgtgc ggcaacggcg gtggcatgcg gtcggcgcca
     1321 attggcgcat tcttctgcga cgacctcgcg gcggtggtcg accatgcggc tcggtctgcg
     1381 gcgccgacgc acgcgcatcc cgatggcgaa gcaggcgcgg tcgcagttgc cgtcgcagcg
     1441 gcacgcgtgt ttgcaggcga gcgcgatcca cgcgcgttac tcgaggcagt tgtcgagcac
     1501 acgccggccg gcgccacgcg cgatggcatc cggcgttcga tcccgatgct gaaggccgag
     1561 cactacacgg tcgcagccga gctcggcaac ggtagccgcg tgctcagcgc ggacacggtg
     1621 ccgttcgcga tctggtgcgc agcgacgcac ctcgaagact tcgtcggcgc atgttggtcg
     1681 tgcgtcgaag tcggcggtga catcgatacg acgtgcgcga tggcaggcgg catcatcgtc
     1741 ggcgcgaccg gactcgcggg cattccggac ggctggctgt catcgcgcga gccgctgccg
     1801 gtctgatagt cattcgatgt attcgaagta ggcgacgcgg atccgcgggc tcgcgatcag
     1861 ggcaaaggtc gcgccgtccg tcgggaatgc cgaggccggt gcgcttcccg agccctcgaa
     1921 gtggcacagc gcctgaccgt tcgacgcgac cattcgtacc ctcgtgtacg gttgcggtag
     1981 gagcttgaag ctgcttgcgg tcgcaccgtc gaccttcgtc gagcctacgc acatagcgct
     2041 gctgcacgcc atttcgcacg caaatgtgcg cgtaccgcga tgcgcgacga tgccgtatcg
     2101 atcggcggcc atccagcgcg atcgcgatgt caccccaatg ccggcgaccc acacccccat
     2161 cggtgtcgcg agcgacagat tctgcagtcc acgatcgctc atcggaaggt cggcgtcggg
     2221 cgcgatcgca tcatcactct gcgtccacgc gaccgagtcg gcttgccagc ctgcgggcat
     2281 atcatcgaag ggttcgaacg cgacacgcac gtcggcgatc gccggccgca cctcgcacac
     2341 cgcaccgata ccatcgccat ccgggtcctc gccgaagtcg ccgacgcctg gacacgcatc
     2401 gcaatcatcc ccgacatggt cgcgatcttc gtcgtgcgtc gacgtcgacg cgagctgcgg
     2461 gcacgtatcg catgcatcac caaatgcatc gccgtccgcg ttcgcttggt cgttcgcgag
     2521 actcggacaa ttgtcgcgat catcggtgac accgtcgcga tcgagatcgc cgccgctatc
     2581 gaagctctcg gtcttctcga ggtcgaagaa ctggttgcag cccacgaggc agacgagcgc
     2641 gatgacgtgc ttcactcgat tacctcgacg tacgcgacat gaacttgcgc gctgccggca
     2701 acggagaagt acgtcgggct ggcagtggtc gaaggcgtgc cgattccgcc ggcttcgccc
     2761 cagctgcagt tcaggtagcc agcggtcgac cgtgccatga ccgcgcgcat gtacggcctc
     2821 gctcctacga tacccacgcc gctgccaccg ctggaaccga acgttaggct gccgttgcac
     2881 gtcgacgccg tgcattcgag cttgcagccg tatgcatcgg cggatcgcgt tgccgtgatc
     2941 tcgacgcgat cgcccgtcgt ccaccgcatc gacgactcga ctccgacgac gactgtgtag
     3001 ccattcgtga cgatgaggct cgagttctcg aggccgcgat ccgtgctcgg cagattcgtc
     3061 gtcggccata cggcgtcttg gccgccggtc cacggcaccc ctgtcgcatg ccattccggc
     3121 gctatcgcct cgaacgtctc gaacaggagt cgtcgattcg gcgggccggg ctcgggatcg
     3181 cacaagtcgc cgatgccgtc gccatcggtg tcgtcattga agtccgggat gcccggacac
     3241 gggtcgcagt cgtccccggt aaagtcgccc tcttcgtcgt gcaccgatgc cgatgcgagc
     3301 tgaggacacg tgtcgcacgc atctccgaac gcatcctcgt cgcggttcgc ctgatcggcg
     3361 ttcgcgagga gtgcgcagtt atctcggcca tcgtcgacac cgtcgtgatc gaagtccgtc
     3421 gcggcgtcga gtgggatcgt gggcgcgagg tcgaacaccg cgttgcaccc ggccgccgac
     3481 acggcaacga gcgcgtacac gccgatcccg gcccatcgca tatcccatcg tcgcacacgg
     3541 cgttattgtt ggcagcgtga gtcacgagga tctgaagttc gcgcttgtcg cgttctccgc
     3601 gatcttcttc gtgatcgatc cgctcgcaaa cgttcctgtc tttttgacga tcactgcgac
     3661 cgacacaccg gatcaacgcc ggcgcacggc cgcgcgcggt gcactcgccg cgtgggtcac
     3721 gctcaccatc ttcgcgatcg cgggcggcct catcttccag gcgttcggca tctcgctcgc
     3781 ggcgttcaag atcgccggcg gcatcatgct gctgctgatg gcgatcgaca tgatgcgcgc
     3841 gcaaccttcg ccgacgcgca cgactgccga agagcagggc gaagcccaag cgcgcgacga
     3901 cgtcgcgatc ttcccgctcg cgatcccgat gctcgccggt ccgggggcga tcgcgacggt
     3961 gatggtgctg atgagccgcg ctgcgtggga tcccgtgcgc acgccgctcg tgttcgtcgc
     4021 agtcagcgtc acgtgcatcg tctgttggct cacgatgcgc agcgcagcca acgccgagaa
     4081 atggatcccc aagacgctgc tgcgtgcgtt cgagcgcatc atgggccttc tcctcgccgc
     4141 ggtcgccgtc gagttcatcg caggtggcgt gaaggagatc atcggcaagt gatgaaccgc
     4201 gcgctgctca cgctcgtcct cgtcggatgc agcaatccgc cgccgccgaa gccgcctgca
     4261 ccgccgccgt cgcagtgcgc gcgcgtcgcc gatcatctcc tcgagctgat gacacccacc
     4321 gcgcaggacg cccccaccga ccagctcgat acgatgcgcc gcacgttcaa cgatcactgc
     4381 aagaacgacg gttggtcgtc gcgagcccaa gactgcatgc tcgccgcgtc gtcgctccaa
     4441 caggtggggg agcgctgcca actgctgctg acgccggaac agaatgcggc gcttgttcct
     4501 cctgcaagct agcccacagt cttctgccgt ctgactcgcg gttcacgtta acgagctctc
     4561 atcgctcgtt ggacctcaaa ccattcggcg cacttccgca cgcgtctcac ggaggcgtgc
     4621 acacctccgc gactttctac ccacaagcgt cgctgactac cgtgaagcca tgggtgacgc
     4681 cgcactgaag gtgcagacac ggttcgcgac tgccgtcgag ctactcaagg ccgtggggac
     4741 ggcgctcgag cgagggtcgc tgcgcctgcc gctccctctc aaattcgaga ctccgttcaa
     4801 ggtgacgctc gtcgctgcca acggcgccga agccgttcgt ggcactgccg agatcgtcga
     4861 gcacgccggc cacgcaacgt ggattcgctt cctctcagcg gggcccgagc tcccaggcga
     4921 cgatgcgtcg cgctgcatgc tcgcggatgt cgacgtcgat gtgacgggcc cgcaagaagc
     4981 tgtcgtcgag cctgctgcgt cgtcgacgag tgaagcgttc gatgcgatca ccgcggtaaa
     5041 cgatcgcccc ccagcgctcg cgtcgatgat gggcacggcg gaggtggtgc cgctcgtcgc
     5101 ggcgccgccc gagccgcagc caacggtcgc tgccaccgaa gcgccgagcg ctgagctgcc
     5161 cgggtcgatt gcggatgctg tgcgctccgt cgaagcctcc gaggcgagca ccgcgccgat
     5221 ccccgtcgag acgatggcgg aggtcgtcgc atcgagccgc gaggtcgtag ccgtcgtcga
     5281 cgaccggagt gacgtttcgt cgtcgaccgg ggcggcggtg tccgtcgtga ctgaggatgc
     5341 ggaggcgatc tacgtcaacg agaagcgcga taccgaagcg agcccaccgc ttgtcgtcgg
     5401 cgagcatctg aaggactccg tcgctgcaga gcgtggcacc gagccgattg cagcgcacac
     5461 gatcgacgca gtcgcagccg agcgcggcat cgagccgatc gcggagccgt atgttcgcga
     5521 gtcacttccc gtcgtgccgc aggcaatgcc gactgcgcga cgaatcgtca tcgcatcggt
     5581 atcgtttgcc gcggtgtgca tggcggtgag catggtcgcg ctcgtttggg cgcacgacgc
     5641 cgtcgccgaa gcggagacga agccaacgcc cgcctcgacg ccggtcgtcg aggctgaccc
     5701 ggtcgaggct gtggcggacg tcgaggcggc gccggtcgtc gaggctgcgc cggccgtcga
     5761 acaagtcgcg gacgagccta cgccctcgac atgcaccctc gagatcgacg cttccgtcgc
     5821 cgagtcacgc gtgaagatcg acggcaagga tcgcggtctc tcaccgacga cgatctctgt
     5881 accgtgcgga cagccggttg acgtcgaagt gcgccacgcg cgctacgcga cgtacgagcg
     5941 tacgattacc gtgactggtg tgcgcgaggc gttgcgcgcg aagctcgagc gcgacaaaac
     6001 gaccgtgacc ctcacgtcgg atccgcctgc gaccgtcacg ctcgatggcc acgtgatcgg
     6061 caagacaccg atgacgacga ccgtctctcg gttcgagcag tcgacctttc gattttcggc
     6121 gccggggtac gagtccgact ggcggcgcat cctgccgaaa acaggggcat cgagcgtggt
     6181 cctgacgctg aagccgcagc gctgaaaaat ggggtcggac cccattcgca ggcatggtaa
     6241 ccaaccgagc ccatgtcgtt gccagcgcgt cagctgttct cgtatcgccc gtattgggcg
     6301 aagcgattcg gcgttgcgcc gtttctaccg acgactcgcg cggagatgga cgagctcggc
     6361 tgggatagct gcgacgtcat cctcgtcacc ggcgatgcgt acatcgatca tccgagcttt
     6421 ggcatggcgc tcgtcgggag gctgctcgag tcgcacggct ttcgcgtcgg cattctcgac
     6481 cagccggagt ggcgcacagt cgatgcgttc aggagccttg gcaaaccgaa tctgttctgg
     6541 ggcgtcaccg ccgggaacat ggattcgatg gtcaaccgct acacgtcgga caagaaagtc
     6601 cgcagcgacg atgcgtactc gccggatggc caagccggcc tgcgtcccga tcgctcggtg
     6661 ctcgtctact cgcagcggtg tcgcgaagcg ttcggcgatg tgccactcgt gatcggcggc
     6721 atcgaagcat cgctgcggcg catcgcgcac tacgactatt ggagcgacaa ggttcgccgc
     6781 agcgtgctcg ccgagacgcg cgcggacttg ctcgtctacg gcaacgccga gcgcgcgatc
     6841 gtcgagattg cgcatcgcat cgccgcgcgg acgcccgtca ccgagatccg cgatatccgc
     6901 ggcaccgcgt tcatcggcaa gcgcgacgaa ttctccgagg tcgattcgac gtcgatcgat
     6961 gcgcccggcc gcgtcgagcc caagccggat ccgtacgcga tggaagccga gcggcaggaa
     7021 gcgccgtgcg cgacgggcga cgcagcagca gcgcccgcag acaacctgat ccagctgcgc
     7081 ccgcgcaagg tcgatcgcga gaagcaggcg atcaggatgc cgagcttcga gcaggtgacc
     7141 gcggatcctg tgctgtacgc gcacgcgtcg cgcatcctgc atctcgagag caatccggga
     7201 aatgcgcgcg cgctcgtgca gcggcacggc aatcatgacg tgtggatcaa tccaccgccg
     7261 ttgccgctga cgacgcagga gatggacgct ctctacgagc tcccgttcac gcggctgccg
     7321 catccgaagc atcagccgac gaagctgccg gcgtacgaga tgatcaagtt ctcggtgacg
     7381 atcctgcgcg gctgcttcgg cggctgcagc ttctgctcga tcaccgagca cgagggccgc
     7441 gtcatccaga gccgcagcga gaaatcgatc ctcgccgaga tcgagcgcat tcgcgatcag
     7501 acgccaggct tcaccggcat catcagcgac ctcggcggcc cgacggcgaa catgtatcga
     7561 atggcgtgca agacgcgcga ggtcgagagc gcgtgccgca agccgtcgtg cgtgttccct
     7621 gatatctgcg acaacctcaa caccgaccac agctcgctcg tcgggctcta tcgcaaggcg
     7681 cgcgcgatca aaggcgtgaa gcgcatcttc atcgcgtcgg gtgttcgtta cgacctcgcc
     7741 gtccgctcgc ccgagtggat ccgcgagctc gcgcagcatc acaccggcgg ctatctcaag
     7801 atcgcgcccg agcacaccga agacggcccg cttcgccaca tgatgaagcc gggcatcggc
     7861 gcgtacgacc gcttcaagga gctgttcgat cgctacacgg cggaggcggg caaggagcag
     7921 tacctgatcc cgtacttcat cgccgcgcat ccggggacga cggacgagga catggtgaac
     7981 ctcgcgctgt ggctcaagcg caacggattc cgtcccgacc aggtgcaggc attcctgccg
     8041 tcaccgatgg cgagcgcgac cgcgatgtat cactccggtt tcaacccgct tcgctcgctc
     8101 aaggcgaaag agcgcgtcgg caccgtgaag ggcctcgatc gccgccgcct tcacaaggcg
     8161 ttcctgcgct atcacgatcc gaataactgg ccgatgctgc gtgaggcgct cgagcggatg
     8221 ggccgtagcg acctcatcgg ctcgggcaag gacaagctcg tcccggcctg gcagcctcgc
     8281 gggacgggca gcgcacatga gggcaggcgc gggagtaagg gcatccggcc gggaactgca
     8341 ctcacgcaac acaccgggtt gccaccgcag ccgcacaaga atcgctcacg caagccccgt
     8401 agcagtcgat aggcgatcac ggactacatc ggggcctgtt gtggctgtgt cgcagactgt
     8461 tatcctcgac gagtaagcga tggggacgcc ttcacctgac gactcaggag tcgtcgcgct
     8521 cggtagcgtg ctggatggcc gctatcgcgt cgaagccatt ctcggcaagg gtggcatggg
     8581 gcgcgtctac aaggccgagc acacgagcct cggtcgcgca gtcgcgatca aagtcctgca
     8641 cgcgcgactc ggcgggagca aagaggcggc gcagcggttt cagcgcgagg cgatggcatc
     8701 gggtcgtctc gatcacccga acatcgtctc ggtcagcgac ttcggcacgc tcgaagacgg
     8761 ctcgctgttc ctcgtcatgg aggcgctcga gggtgagcca ctcggcaacc gcatcgaacg
     8821 cgacaagcgt attccctggc aggagtcgct cgtcatcatt cgcggtgtgc tcgccggttt
     8881 gcgccacgcg cacgacaagg gcgtggtcca tcgcgacgtg aagccggaca acatcttcct
     8941 cgcgaacaaa gacggcgagc cggtcgtcaa gatcctcgac ttcggcatcg cgaagctcta
     9001 cgcgggaaat gcggacgatc cggcgtcgac gcgcgcgggt ctcaccgttg gtacgccggc
     9061 gtatctgtcg cccgagcagg cggtcggcgg cgcgatcacg cccgcgagcg atctgtattc
     9121 gacgagcgtc gtgctctacg aaatgctcac cgggcggccg ccgtttctcg acgacgaacc
     9181 gctcgcgatg ctgaccgcgc acgtgagccg cgagccgccg ccgttctccg aagtggcgcc
     9241 cgatctacag attccgccgg gcctcgaagc ggtgatccag cacggcctcg cgaaagtgtc
     9301 ggcggagcgc atcagctcgg cgaacgaata tctcgggcgt ctcgacgagg tcacgcgcgc
     9361 cgctggctac gacctcggga tgatgttgcc gcgctcgtcg ggctcgctcg cgatccctgc
     9421 gggaccgcac gccgtcacgc cgtcacccgg ctttctcatg acacctgcgc cgttccccgc
     9481 gcatcccggc aacacacccg cggcgggcgt tcacgtgatg acaccgccgg gaggcgtgca
     9541 cgcgatgaca ccgatgccgt tctcgtcgga gatgggcacg gcacccacgc aatcgctcga
     9601 ctcgatgccg caggcagcgc gcatggtttc ggtcgcggat atcgcgagca aagagccgct
     9661 gccgtcgaag tacaagaaga tcggcatcgc gatgatcgtc atggcgatcg ctctctccat
     9721 cttcgcggtg gttcgcagca gcgacaaagc gggcgcgacg cgaggtacga cgggaccgat
     9781 tcccggtggc cctcccatcg acaacgagac gcgcctcaaa gcggcgctgc acgatctcga
     9841 gaccggcaag acgtgtgcgg atcgcaaagc ggcgattccg acgctcgtcg acatcgggga
     9901 tgaacgcgcg attccttcac tcaagaaggc gcgctatcgg atgcgcggcg gcgtcatcgg
     9961 tatcggcgac agcaacacga acgcgtgtct caagcaggat gccgagatgg cgatccaagc
    10021 gatcgcccca cagtcggact gatcacgccg gcgggcgcgc tttccagtct ttctgcgaga
    10081 acgcttcaaa atcgatcacg agacagatcg gcttgtcggc cgcatcgacc gcccaatagg
    10141 cgacgaactc gccattgcca cacggcagcg cgagcgcgcg acgtccgtcc gtgagtggaa
    10201 cctcgacggg gttgagccca ttcgcggcgg caaccgcgag catgcccggc gaatccttct
    10261 cgcctttgcc tgcatcgagc agtgcgagcc aacccgtcga gacttcggtg cgcggcaact
    10321 gatcggccga cttcggcggc ttctggccac gccagtgcgc gacggtccag cgcgtgatcg
    10381 gcgggcgacc gacgtggatc gtgatcgcgg cgatgcgctc tttaccgtcg tcggtgcgtg
    10441 cgatcgagag catcgcgcgg aactgcccgt tcccgacggg gcgatcgata accttgaacg
    10501 acttcggcac gcccgggtcc atcaccgcga gcgaccccga cgtcacgggc agttggatcg
    10561 gcaaccagcg gacatcgatc ttgttcgcgc cgaaccgctc gccgtagcgc gtgagccgcg
    10621 cgatgtcgcg gggagctgcg agctcggtgc gctgcgccgg ctcttcgact ttcggcggtg
    10681 gcgggggcgc cggcatcgaa ggcttcgtcg ccttcgcggg cttgatcgca cgaacgaccg
    10741 cgcggccggt cttcttgtcg acggtctcct tcgccttgag gatcgtcggc ttcacgacgt
    10801 tgacgaccgc tgccttcgcc gctttgcctg cggccttcgc ggccttgccg gcagccttca
    10861 ccgtcttcag tgccttctgc gcggcagctt tccccttctg tgacttgctc gtcttcgcca
    10921 cggcgcgacc gtaacacggt cgtcgttaga tccatccgcc gggccatgcg ttcacggccc
    10981 catgaggcaa gtaacttgga cactcgcgac cctggttggc ttgggtggcg ttgcagcggc
    11041 gaatccaggc gaatcgcccg gtgtggactc gtcgaccgtc acgctcggcg tcgagtcgcg
    11101 atcgcagcgc ggcgtcgcac aggattacct cgtgctgcca agtggcggcg agctcggtgc
    11161 gcagatgcgg ttcatcacgg cgccgcgtgg cccgatcttc gaaggcgagg gcgagcagaa
    11221 gctcgagttc accgacctcg cattgttcgg ggtgtcggcg cgctggtcac tgttctcgaa
    11281 gctcgagctc tcggcgtcgg ccacgttgct cccgaagcag ccggcattca ccgacgagaa
    11341 accgtggcag agcgtcggcg ttggcctacg cagcccgctc ggtcgcaatg cagcggtcgc
    11401 tgtctccggt ggtggcgggc atctcatgag ccacgccggg atgtggacgc gcgagtcgct
    11461 catgctcgag tggaagaagc cgatccacga agacttcctc tcgttcgaca ttcagggagg
    11521 cattcagggc ctcggcatac aggcgccgaa aacgacgaag tcgaccgcga tgatcaccga
    11581 ggtcgcgatt cagacgaccg cgctgtttcg cgagccgcac ggcaaatggg gcgcatggct
    11641 cggcatgggc tacgcggtgc ccgtacagtt ttccggaaaa gatccgacca cggagatgtg
    11701 gatcaaccca caacctcgtc tcgacttcca catgggcacc gtgctcgcgc tggttcccaa
    11761 gtgggatctg ttcgtcgact tcgcggtcgt cgatcgcggg gagctgagtg acccgacgac
    11821 ccggctgccg atcctcgatg gtgggttcga tcaacgtcag gtcatcttcg gagtgactcg
    11881 ccacatcgag ggctcaaagg cgcgcagcta cgacgactat ggcgatgacg acggcgcgat
    11941 tcgggtgggt actctctagg gggtaaatct ttaccctggg tgagtgacgc cctacccagt
    12001 aacgccccta cctgagatgg ttatctcgtt ggaattgtag cggttcgtgt agaccgcgca
    12061 gccgcgcacg gttggatcga gggttgtagt tacccccagc gtgcaggcat tcggctccaa
    12121 gacctccccc gccgctcgca accgtgacca gctgatccgc gagcacgcgg acgtcgctcg
    12181 tcgcatcgcc cttcggatgg cacgtcgctg ccccgaatgg atctcgcgcg aggacctcgt
    12241 gtccgcgggc atggtgggcc tcatcgaagc ggccgaccgc tacgacgaga gccgccagga
    12301 gccgttcatc gcgttcgccg agcaccgcat ccgcggcgcg atcttggacg agctgcgccg
    12361 tggcgacatc atgccccgcc gcgttcgcca actcgcccgc aagatctcgg cggcgatcaa
    12421 agagctcgag aaggacggca cgcaggcctc cgaccagcgc gttgccgatg cactcggcgt
    12481 cccggtcgag cagtaccgca cgggcctcgc tcagctcgtt cacgtcgaga tgcagccgct
    12541 cgatggcgag gagacccgcc tcgtcgcgca agacgtgctc cccgacatgc aggcggatca
    12601 ccgccagatg ctcgctcgca tccgcagctc gctcgatcgc atggaagcgc gcgacgtcac
    12661 gatcctcggc ctgcactacg tcgaggacct cacgtaccaa gagatcgccg acacgctgaa
    12721 gatcacgccg tcgcgcgtgt gtcagctgct gtggcgcgcg gtcgagcggc ttcgcacgca
    12781 actgggcgcc gaagtcatgg cggcggcagc gtgagtaccc gcgtcctcgt cgtcgacgac
    12841 gacccactcg tgtgtcgcgc gatttcggtc gtgctcgcgc gcggcgggtt cgatgtccag
    12901 aacgcgaacg acgcagcacc ggcgctgcag atcgctgcgc agtggacgcc ggatatcgcg
    12961 gtggtcgact acaacatgcc gacgtgcggg ctcgctctcg tgcgcgagct caaggcgcgg
    13021 ctcggcgaca acgtgttcgt cgcagtgctg accggtgacg atgatgacga gatgcgcgag
    13081 aagtgccacg caggcggcgc agacgccgtt ttgatcaaac cgatcgcgcc tgtcgagctc
    13141 aggcgccggc tcacggctgc ggccgtcgcc ctcaagacgc tcgccgcagc gtcttgatcg
    13201 gatggatttc caccttcgac catgcgagcc ttcctcctcc gcggatggct gaggcgccta
    13261 tcgcaagagt gatggtgctc gaagacgacg aggacgtctc gcgagcgatc gcgcgcaagc
    13321 tgcgtgcgga caactttgcc atcgaagagt cgatggaccc ggttccggtg ctccagcgcc
    13381 tcgagcaggg caacgccgat tgggacgtcg tcatcctcga tatggggctg ccgggcatga
    13441 cgggcctcga agtgctgcac cggttccgcg aagcgggatc gtttgcggcg tgcatcatgt
    13501 tgacgggcga tcgcagtgca ggcaccgcga cggagtgcat gcgcgcgggc gcgttctatt
    13561 acctgacaaa gccgttcgag ccgttccagc tctcgtcgat ggtgcagtcg gcggcgcgct
    13621 attcgcagat gcggcgtcag ctggcgaccc gcgagacgaa agaggacgtc tcgctcgtcg
    13681 gcgcgtcggc agcgatgcgc aagctgcgcg cgtccctcga tcgcctcggc gatcaggacg
    13741 taccgatcct catccaaggc gagagcggca cgggcaagga gctcgtcgcg cgcgcgttgc
    13801 acgagcgcgg tgcacgtcgc cgcaaacggt tcgtcgcact caactgcggc gcgattcccg
    13861 agacgctgat cgacagcgag ctgttcggtc acatcaaggg cgcatttacc ggcgcgacga
    13921 cggatcgcgc aggcgtgttc gtcgaagccg atggcggcac gctgttcctc gacgagatcg
    13981 gtgacatgcc gctcgccgtg caggcgcgcc tcttgcgcgt gctgcaagag agcgaggtac
    14041 gcccgctcgg tggcagtggc aatcgcaagg tggacgttcg cgtgatcgcg gcgagccacg
    14101 tcgatctcgc acaagcggtc gagcagggca ggttccggca ggacttgttc taccggctca
    14161 acgtcgtcgt cctgcgcgta gctccgctgc gcgagcgcct cgacgacctg ccgctgctcg
    14221 cagcccactt catccgcaag cacggtggca gcaagcctcc aacactgtcg cccgacgcac
    14281 tcgaagcgat gatggactac ccgtggcccg gcaacgtgcg cgaggtcgag aacgcggtgt
    14341 tacacgcgat cgcgctgcat cacggagatg ccatcggcgc cgagtcgctt cccgcgacga
    14401 tttctggacg ccgcgcgtca gcgagcagcc cttcgctgca gagcgtcgac gacgacgccg
    14461 cgaatctcga gcctctcacc gaggccaagc gccgcgcgag ctcggccttc gagaagcgct
    14521 acctcacgaa agcgatggaa cgtgccaagg gctcggtctc tgaagccgcg cgcctcgcag
    14581 gtctcgaccg cacgaacttc cgccggctgc tccagcgcca cggcatcgat cccgcgtcgt
    14641 tcaagtagga cgatctttcg cagcgttttc cgaacgctct tatccctccg ctgcccgaaa
    14701 caagttcagt gggcggccga accaagaaac atgagggacg aaattctcga cgaggcgaag
    14761 gcgctcgagg cctttggagt gctcacgcgg agcgcgacgc acgatctcaa taaccagatg
    14821 gctgcgatca tgacgttcgc agacctcgtg ctcgaggcac tgccggagtc gcatccgatt
    14881 cgccacgagg tgcaacagat ccttgccgcg ggcggacgcg cagtcgcgaa gacgcgcgag
    14941 ctgtcgatga tcgcgcgccg cctgtcgccg atcggccgga gggcctcgtg aaccacatgt
    15001 ccatcgtctc tgcgatcgag aagctgctcc tcatcgccga atatcgcgac gacgttcgtc
    15061 agctgtcgct cgacgtggtc gagatctcgg gtcagattca gacgatcctc atcgtcgtcg
    15121 agagcgcgat gcgcaacgtc ggtcacgacg ccgcactgcg cgccgacctc gaagagattc
    15181 gtcaggcagc cgtgcgcgcg atcatgaagg tcgagccgct gtcgctcggc tacggcgtcc
    15241 tgcgcgccgt ctgacgaaca gcagcgcgcc gacgacgaac agcccgtcgc tccgtcccgt
    15301 gctcgtcgag cagccgcatc cctcatcggc cgctgctccg tgcacgtaga tgtgactcgt
    15361 cacgacaagc cgctgattga gatcgttgat cagctccgct cgataccgcc gcagctgcac
    15421 gccgggcgta tccctgaatg cgatcttcac gtcggcggct gtgacgggtt tctgatcgat
    15481 cttctcgccg tcgcgccaca gctggacgat cagcggtgcg ccatctggat cggtgccgac
    15541 gacatgcacg gtcgcatgca cctcgccgag gtcttgcact tcgtcgccga tctctgcagc
    15601 ggtgccatcg gcgcgcgtga tcgtcagatc gatcgtcggg tcctcggcat tgcgcagcat
    15661 caccgccgtt cggccctcgc ggatcgcttt cacgatcgcc gcttcgctca gattgtcggc
    15721 gaggacgagc gtcgtcgggc tgccgatctt cgacgccgtc ggtccggtat cggtgccggc
    15781 gcgatgatcg tcgctgccgc cgacgacggc gatgtgatgg tcagcgcctt ccagctgatc
    15841 ccacagcgtg acgacgcgcg gcacgaacgc ttgaatgccg atctcgaagt tgccggtgat
    15901 cagctccatg ccggagacct tgtcccaggg cgtgtcgtcg acgtgcttcc acgagcagcc
    15961 gatgcactgc gtgccgagat cgagcacggg atgattgacg atgaagacgc cctcctgcgc
    16021 tgcaacgtcg tcgacgattc cctgcaccgt gcggccgttg tagccaagcc gatgatcgac
    16081 gtagtcgtgc gtgccgacgg aattcccgtg gcccgagtac gtcgtgatct ccgcgccgcg
    16141 cagaaacaga aagtcgggat acgacggctg cgccgctgcg atcagggcgt gctgcgagta
    16201 cgtgttgtga tcggagagat tgacgaagtc gaggccgcgg ctcttcgcga gagtagcgat
    16261 cgccgcaaag ctcgcgttcg cgtcaccgct ctgcaccgag tgcacgtgaa agtcgccctt
    16321 gtaccagcgg cgctccgtcg agagcacgac cggcgcgaac gcggcgcgcg cctgcggtgt
    16381 gagtgtggcg gtatcgctgc aggtgacgtc gatcgaatag tggccggtgc ccgcaccgtc
    16441 gagcagtgcc ttgccgatcg agaccgtcca cgtgccggcc ggaatgggcc ccggcagata
    16501 gctgcgcgat gattcgtcga cgccgatgat cgcgtcttcg gtgttgccgc cgccccagcc
    16561 gcgaaaccgg ccgccgggct cccacacgcc ccaatcgaga atcacatcgg cgctgccgtc
    16621 ggtgtgcgtg atctgaatct cgacggtgcc gaccggaacg tcgaagggga catcgacgta
    16681 atcgccgccg gctgcagtga cgtcgccctc gtaatgcatc gacgacaaca cgagcgcgat
    16741 cgcaacgagc cccatgcgtc gatcttacta cgcgggtagc ccgacccact cggcggagcg
    16801 gttccacagc tcgcgggcga ggtctgcgtc acgcgcgaga cggctcggct ttcgctcgtt
    16861 accgtcgagc cagtagaact tgccggtgtc gttcgcaagc gcggggtcgg tcgcggcgcg
    16921 cagcatcgag acggcgcctt gctcgggcgt cagcatgcgc atcgctttct tcatcgccca
    16981 gcgcagaggc tgcgggacgc cgcgccagac gtccgtcgcg acggtaccgg gatggaccgc
    17041 gtacgtcgtg acgcctgtgc cttcgagctt ctttgcgagc tcggcggtga acaggacgtt
    17101 ggcgagcttg ctgaccgcgt agtcgccgag gccggcgaac gacttcgtcg gcttctgcag
    17161 cttgtcccag gggaacgcgt tgcgcacttg atagtgcgag acggacgaga cgttgacgat
    17221 gcgcgcgccc ggcgtctgct tgattcgatc gaggagcagg cgcgtgagga gatagtggcc
    17281 gagatggttc gtcccgaacg tcagctcgaa gccttgcttc gtaacgccgc gctggccgcg
    17341 caagccggac gcgaagcccg cgttgttgag gaggccgtgg atcgggatgt cgcgagccag
    17401 cagctctttg gcgcacgcgc gcacggagtc gagatcgccg agatcgagct tcgcgtattc
    17461 gacggcgtcg ttgccggtcg tcttcttgat ctcgtcgatg accgggcggg tcttcgcttc
    17521 ggagcgggat gcgatgatca cgcgcgcgcc gcgactggcg agatcgcgcg ctgcgaccat
    17581 gccgatgccg ctgttgccgc cggtaacgat gaagttcttg ccgacgagat cttgggccat
    17641 gcgggcgaca tgatacgcct tgccgcatga cgcgttggtt gctcgcgctc gtaacggttg
    17701 ccgcatgcgg cggccgcacg accacgccgg cgccacccgc gaagcccgct ggcaagccgc
    17761 tctatgatcg catgggtggc atggacgtga tcgacgcggt cgtgaaggac ttcgtcgagg
    17821 tgcggatcgc gcaggacgac cgcattcgcg atcgcttcaa gaccgccgac cgcgcgcact
    17881 tgcagatgcg tcttgccgag cagatctgcg agctgagcag tggaccttgc aagtactccg
    17941 gcaagcacat gcgcgatgcg cacgcgggaa ttggcatcgg cgacgccgag ctgcgcgtgt
    18001 tcctcgacga tctgcggcga tcactcgaga cgctccgcgt tcccgcgcgc gagcaagacg
    18061 agctgctcac agcgctcggc aagctccacg acgagatcgt gcaggctcag taaggcgcgc
    18121 cgccggagga gtttccgccg ggcccgtagt tacagacgag gttgcctttg tactggaggc
    18181 ttccgcacgt gacgttcgcg caaccgatgt ggatcgacgt tcgccacacg atctgcgtgt
    18241 agtgaccgca cgtgccggag cagccggtgg gatacgtgaa cttcgatttc tcgctggccc
    18301 acgtcgcgac cgcttgctgc gcggtggcgg ctccgcccga cgcgaagatg ttctcgccga
    18361 cgtacgagta gcccgccgca ttcgtgcggt acgcctgcga gctgtggtcg acgaacccgt
    18421 tgttgtcggc gtcgatgcat tgcgacgccc aattgttcgc gagcgtcgcg agcgctgggt
    18481 cccacaccat cagtggcagc ggtccagccg caatcccggt cgtatcgacc gcggcgcgca
    18541 ccatattgtg agcgtcggtg atccccgcga ggttcattgg ctcgcccgtg ccggcaggag
    18601 cgtccatgta cacgtgattg ctgccgctgc ctgcggcgtc accggtcatc atcgacgagt
    18661 cgatgtcgcc atcgcatgca gcgagtagga gcgccgcaac aacaagcccc ttcatgcgcc
    18721 aagaataacg tggtacgtcc cggaccggag gcacaaagca agaccgacaa gcgacgacac
    18781 actccgatgg atgcagggaa gctggtgaaa aagccagcac agcccccgct actgtgaccg
    18841 gggacgacgc agcgaggccg gatggccagc cactgggaaa ctgggaaggc gctgcgaagg
    18901 acgaaccgga agtcaggaca cctcactgtc ggaaacaacc tcggggtgta ggacgcgtcg
    18961 cgttcgtcat cccgccgtta cgtggggtga cgaatgaaca aaaccggatc cacattgctt
    19021 ctgctcgcgc tctgcgcgag agcctccgcg caagacgcgc ctaccggcag cgctgcgcag
    19081 gaagtcgatc cggaaacgat cgtcatcatc gatcgcgcgc ccgatccgga tgccgcgccc
    19141 ggggctcgcg accgcgaccg catgctcggc gatgcgccgt tcgtcacgat cgtgcatgcc
    19201 gacgatcatc cagggacgac atccgtcgcc gatgcggtgg gcgcgacggt gggcgtgcaa
    19261 tcgcgatcgc tcggcggtct cggcgcatac gagtcgatct cggtgcgcgg cgcggcgccc
    19321 ggtcacacgg tcgtgctcgt cgacgggatt ccgctcgcgc gtctggccgc ggtgacgacc
    19381 gatctcgggc gctattcgct ctcgtcgttc tcggaagtcg agctctatcg cggcgcggtc
    19441 cccgtcgagc tcggcggtgc aggcgtgggc ggtgcgctca atctcgtcac gagactcggt
    19501 cgcggcgagc gcggcgagag gatcacggcg tcgctcggcg caggctcgta cggcgcgcgt
    19561 cacctgcgtg cgcgttatgg cgaccgccac ttcggccgca tcgagtccgc gtcgacgctc
    19621 ggctatcagg cggcgaccgg cgactacacg ttcttctccg acaacggcac gttgctgaac
    19681 aagaacgacg acacgtatca ggttcgcaag aacaacgggt tctcgcagct cgacgccgca
    19741 acgcgcctcg gcaatctcga tcgcagcagc gtcggcggcg tgcgcctcgc ctggaagaac
    19801 cagggcctgc cgggcagcgc gacacaacca tcgctcgaag cgggtatgtc gacgctcgac
    19861 gtgatcggcg atgcgaggct cgatctgcac gcaggctcgg cgacagcgcg gcagctcgtg
    19921 tacggcctcg tcgagaaaca acgtctcgat gatcccgctg gcgagctcgg cctcggttcg
    19981 caggagcgcg gttatctgac gctctcgggc ggcgcgtcgt cgacgtggca actgccgctc
    20041 ggcgtgcatc gcgcgaccgc aggcatcgag ctgcgcgccg atcgcttcaa agacagcgac
    20101 ggcaccggca tgcggcagcc attcgtcggc gatcgagaag gtggagcggc gctcgcggcg
    20161 atcgacttca atctcgatcc gacgatcgtc gtcacgcccg cgctgcgctt cgacctcgtg
    20221 cgcaccgcac cgacgccgat gggaacggga ccgaccgcag ggcagccgat gccgatccgg
    20281 cgcgatgccg tgccgagccc gcgcatttcc gcgcgagccg cggtgaccga ggacgtctcg
    20341 atcaagacga gcgccggttg gtacgtccgc ctgccgacgc tcgtcgagct gttcggcaat
    20401 cgcggcgcga tcctcggctc gccggatctg ctgccagagc gcgggccgac cgccgacgcc
    20461 ggcgtcgtgt gggcgcctgc gaaagcgcgc ggcgatttcg atcgaatcct cgtcgaagcc
    20521 agcgtgttcg gtacgcgcag tcacgacacg atcgcgttca tcaactcgag cgggttcatc
    20581 acgcgcgcgg tgaatctcgc gaacacgcag acgtacggcg gcgagctcgt cgcgtcgatg
    20641 cgcatcggcc gcgcgctctc ggtcacggcg aactacacga ggctcgtcac ccagcagctc
    20701 tccgaggacg ttagcttcgc gaacaaggct gtacctcgcg agccggggca cgccgtctac
    20761 gcacgcgcgg atctcgcgca tcgcctgttt caccgacgcg cctcgatctg gctcgatggc
    20821 agctggcaat cgtcgacgta tctcgatcag gcgaacttca tgatggtgcc agcgcgcctg
    20881 ctcgcaggga cgggcgctcg catcgagctc gccggtggcc tcgccgcgtc gctcgccgtc
    20941 gagaacctcg ccgacacgcg cgtgcagcag ctgccgctcg atcctccgcc tcgtcccgat
    21001 ctcacggcca cgcccaccgc tcttgccgac gtggccggct ttccgcttcc cggacgcacg
    21061 gtctacgtgt ccctcgattg gagctactaa atgcgtcgaa gtatcgttct ctctctcgct
    21121 ctctctgtcg ttctcgtcgc gtgcggcgac aacaaggatg tgcccgatgc gcgcatgggc
    21181 atcgatgcac caccgcccga tgcgcacatc gtcacgcctc gtgccgtcgc ggtcgcgggc
    21241 gacttcggtt cgcccggtgt cggcaccgtc agcaagctag aggtcaccga cctcatgatg
    21301 cggcagaacg tcgtgcaagg cgcggcgcaa ggcgatccgg cgctgcgcgt gttcggtaat
    21361 cgcgtctacg tcgtgaatcg gttcggcagc aacaacatca cgatcctcga cgcgaagacg
    21421 ctcagcgtcg tcgatcagat gtcgacgggc gcgagctcga atccccagga cgtcgcggtc
    21481 gtcgacaaca agatgtacat ccccgccctc ggtaccgcgg gcgtcgtggt gctcgcgccc
    21541 ggcagcccga cgccgcaggt gatcgatctc gcgacgccgc tcggcgacgc cgatggcaag
    21601 cccgactgcg tctccgcgtt cgcggtcggc aagtacgtct acgtcgcgtg tgacatgcag
    21661 acgaacttcg ccaacgtgct cgattcgaag atcgcggtga tcgacaccgc gaacaaccac
    21721 gtcgtcggcc aagtcatcac gccgtacaag aacccgcttg gcctcttcgc gcacgcaccg
    21781 caggcggccg tctacttcgg cgacctactg atcccgctcg tgccgaactt cggcgatctc
    21841 acgcagggct gtatcgctcg catctcgacc gtcaaccctg cggcaccgac ggcggcatgc
    21901 ggtccgacga acgcggagct cggcggcttc gcgaatcgcc tcgccgtcga cagcgagaac
    21961 aacaagctct acatcgcagt cgcggagagc ttcacgagcg gcaagctgcg cacggtcgat
    22021 ctctcgaacg gcgccctcgc agcgggctcg ctctcgaaag caggccagct gatcgtcgac
    22081 gtccaggtct gccctggcgg cgatgtcgtc gcatccgatc agacgacgaa cgcgaccggg
    22141 cttcgcgtgt ggcgcggcac gaccgagcgc acgaccgatg cgatctcgat cggtatgaat
    22201 ccaacggtca acggtctcgc ctgctacgac ccgtagtgtg aagggcgctg cagctgtcgc
    22261 ccagccagct tcagcgacat gcacagcttg tagagcgctc gcagcgcatc gaggcggctg
    22321 ctgacggcgc ggtcagacat gtccggttca ggtttcgtcg tcatcggtgc cgtcgagctt
    22381 ggctaggtcg gcgaggtcct gcggtcggcc cgccaatcgc ttcatcgtag caagcccgtc
    22441 ccgcgagact acccaaagct tccggccgtt ccagtcgacg ctggttcggc c
//
LOCUS       DATLBZ010000274        12292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_300414, whole genome shotgun sequence.
ACCESSION   DATLBZ010000274 DATLBZ010000000
VERSION     DATLBZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12292
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300414"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /locus_tag="VMZ53_14485"
     CDS             <1..1672
                     /locus_tag="VMZ53_14485"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS29707.1"
                     /translation="GVPNGQWIADAFSPGYISPGGVELTAGQGIVELAMARGATIEGR
                     VLDGEGRGIENATVRAITAGQNPTEISALVEQDQLRRFSGRTAAPAAVSTTNSFNGDP
                     DFIPRGELGVTVGPIPPIPPPGVVAARPASVIDPKAASASLVGEPPPLAVDPDRASIW
                     TTGRDGRYRIRGLSKTKLSVLAAAAGYAEARSKDVSIGNGQVVLGVDIILTAGTFIFG
                     KVADKRGAPIVGAQITAQPELGAPLDAFSDADGMYRLGPISGKIDLGANSYGFVETHR
                     RVDLAPAKGRLAAEQREDITLEVADAVLAGTLDDAAGAPIPGANIEVISGPGSGRHAV
                     VAADGTFNIDMLPAGPLRLRIDHPQYPSDELDATASTTGQRVRLRLALGGQVEGVLLD
                     SATGKPLGGMTLDARGPAGRTADTTSDDKGLFKLGPLKPGKWKLDIKLPGYLAQTRDV
                     DVPVSRAPGETSVRDIRIELQRGALVGGTVRDRRGQRVAGAKVTVRRIDGTGEPVEED
                     TNAQGEFLIHDAPTGELMVAAQSGDAGGSTHVTVRPGAEVLSLTIEIR"
     gene            2022..3101
                     /locus_tag="VMZ53_14490"
     CDS             2022..3101
                     /locus_tag="VMZ53_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29708.1"
                     /translation="MRSLIFVAVFLASTTAIAGVPTRMPVQGYLTDTNGTVVDASVQI
                     QFQIYATATGGTALYSENQAVTVDDGNLTVYLGSNVPLDMSLFRGGTAYLGITLQGEA
                     EMSPRLELATVPFAAVAQYAEETASVPTGAVMMFDLDTCPAGWTALDAARGRAIVGVP
                     AGGTRGGTVGTALSDVEDRAHSHSVGPFSTTTSSAGNHVHNVPLSFSTNQSTLDLTHV
                     HTVDPPPHQHRWASYFIDPTSLNSVFHTYAANDGSTTGAITLQDWDNGMDSAGAGYYP
                     VAAPAAAATGSTSFYYTQTTDMGSMTSSTANPPSTHAHQVSSNATAQLGGTHMHDVAI
                     AASASGGATTGAVMPYLQLLICRKN"
     gene            complement(3098..4519)
                     /locus_tag="VMZ53_14495"
     CDS             complement(3098..4519)
                     /locus_tag="VMZ53_14495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29709.1"
                     /translation="MRRAQQLGRYHLLDRIAFGGMAEIYRAKTFDANGTPHLVAVKRV
                     LAHLAEDDDFIQMLVDEAKIASVLRHQSIARVYEFARAHGEYFIAMEHVDGKDMRTVL
                     ERCRAKKKPIPPEHAAYICAEVGGALHAAHSAVDSRGRPLRIIHRDVSPSNIICSYAG
                     EVKLCDFGIAKATLSRVTTKTGVIKGKVKYMSPEQALGRKLDHRSDVFSLGSCLYEML
                     TRIPPFTATNEMDLLIKVRDAKYRPIAELQPGIPPELEQIADKCLTRSRANRYQTAAE
                     AEGDLRAFLRKFMPNYSRSHLGRFIRKMFNTEIERELRMLEEFVMADEPSDDVGESLM
                     LRDEDFEPPEVPFQPKPTSVSVALAPSNDFSGDSNFTTMPKVDQDGRRIDSLGEDIHQ
                     QQTMILDSRGRRAVPEAPPPKRQQQSTARGQPTPRGRTQPPPVPPQARKPSGVKPIYD
                     EELHAAPTMIIDINRMRRQRGRG"
     gene            4580..5170
                     /locus_tag="VMZ53_14500"
     CDS             4580..5170
                     /locus_tag="VMZ53_14500"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HUS29710.1"
                     /translation="MVRALGISLLLISACGKSASKQDNTKATTPSGEEVIQVPGSKGG
                     PAVGAVEGAGPDDGNVQATGAGAPPNPAFFLKPEEGTLTVDKAEGKAGAESTAKLSVA
                     PAKGYHMATDYPIKLELEPTAGVTLQKAKLEAGGRNKVQGDAETLSEQSLAFAVKATA
                     EKAGTYEIKGTFKFGICEKDSCHPKKQPVTITVAAN"
     gene            5187..7418
                     /locus_tag="VMZ53_14505"
     CDS             5187..7418
                     /locus_tag="VMZ53_14505"
                     /inference="COORDINATES: protein motif:HMM:NF018504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29711.1"
                     /translation="MADLPPTVKDTLVGLHQSVREDFTRNRRVLSFSEYLALAFAEPA
                     RQLRSSTQYIVDCFDHYGAEKVKYPWGEVTRFKLFDAPWAGGEDRLFGQEQVQNTTYR
                     ILRTFVQDGRPNKLILLHGPNGSAKSTFIRCLGRALEHYSTLDEGALYRFSWIFPAQK
                     STKGGIGFGGGGQSIDTLDSFAYLPDDQIDARLGDELRDHPLLLVPFPERQKLIGKLL
                     DGKAFHLSDYLRGGALSPRNRAIYDALLASYQGDYVQVLRHVRIERFEVSHRYRQAWV
                     TVEPQLSVDASERQITADRTLAALPPSLQTVSLHEYGGEVVGANRGMIEFDDLLKRPL
                     EHYKYLLTTVERAALSMQSATLFLDLCFLGSCNDIHLSAFREIPDFQSFKGRIELVRV
                     PFILDVNHEEALYQERLQESAGGRHIAPHTAYVAALWAVLSRMRKPQIERFPSTLAEV
                     IGRLTPLDKVELYTSGEVPEDLSPDRARELHAHIKDLFHESDTYPIYEGRVGASPREM
                     QTVLLAAAASPRYEYVSPLVVLDEIQELCKQASLYEFLRQDVQPGGYHDHKKLADVAK
                     GRLFDVIDDEVRVAVGLVEESEYTRVFERYVTHVMHSIKKEKVRNTSTGRQEDPDENM
                     MREVERTLEIPGRAEDFRQSLIAKIGAWSLDHKGQKPVMADIFADLLRKLRDAYYERH
                     KKVIAKGITDLVVLLSGNEGHLSSADRTRADAALATMITRYGYTRESARDLVGALASM
                     RYR"
     gene            7472..8776
                     /locus_tag="VMZ53_14510"
     CDS             7472..8776
                     /locus_tag="VMZ53_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUS29712.1"
                     /translation="MLRGIVCICVLAVAGVAAADAPRMLKGPYLQDLAPTSITVMWQM
                     EPQQAATLVVEGPGGTRTIDVKADRVAEAQIAGLTPSSRYRYRVIAGDQQWQGEFATA
                     PPIGKDVPFSFVVFGDSRYGEAHHRRVVNRMSQEVPDFMLGTGDMVDEGYRQEQWQSF
                     FDIENEMLRDNVYFPALGNHDRQGRGRTADSYRAFFSVPENGNETERYYAFTYATSRF
                     LVLDSNLYSFALTDQTSWLERELIAARQDPAIHHIFVVMHHPPFSISLHGGAVDLRER
                     WTPLFEQYNVTAVFSGHDHVYTRAMHNGLHYFISGGGGAPLYPRRPKSNPIDVAAVKK
                     FERVLHYLRVTITGNRVEVTAVRADGTTIETTSWKDGPEPTLEQPKLSATGAPPAAAA
                     TAAPTAPRAKVPPPDDGNMLWIGLGGLVVVLGAAVFVVRSLR"
     gene            8795..9289
                     /locus_tag="VMZ53_14515"
     CDS             8795..9289
                     /locus_tag="VMZ53_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29713.1"
                     /translation="MRRLVLLCLVGCGGEPPECGTSLAEAIFVETALDAYDHAFGPTA
                     QLTATCPGGGTSTVEGASGPSATAPRVDDYKVTYAGCATLDARLTLDGVVEIRGTWVG
                     GTDAAYCDECTITSSALMIRGTEDRCDDSHIARTCPVDFKVKGSASNVPQRYEGSICD
                     FDFP"
     gene            9300..9824
                     /locus_tag="VMZ53_14520"
     CDS             9300..9824
                     /locus_tag="VMZ53_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29714.1"
                     /translation="MRRCVGVGTVLFSVMVAVACAEPEEPECTSSIAFDIHYLYVQAT
                     NATTRSGGSGSNWTCPQGGSATVTETSTTNNATQTNDSQLTFHMCSYVMNGFDIRLDG
                     PLHAHNVSNIVGNLVYCNTCELTSPGLKIYGSELLCNADPIDATCEVSFTIKNESTQS
                     MGDYAGSICSLTFP"
     gene            complement(9811..10389)
                     /locus_tag="VMZ53_14525"
     CDS             complement(9811..10389)
                     /locus_tag="VMZ53_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29715.1"
                     /translation="MFECRHQPVHLPDDVARELVAAYSEPHRAYHNLTHIAELLGWFD
                     RVADDIGWKDPAAVYAAIVFHDAIYVPGAKDNEARSADWARRAGFGDDVAQLILMTMS
                     HTSHGDAGHDAAHFLDADMAIVGAPAEQFAAYDAAIAREYSHVPPDAFRAGRKAFLAG
                     LAKLPRIFITEYFHSRLDAQARANLAAAIRGM"
     gene            10413..11762
                     /gene="hisS"
                     /locus_tag="VMZ53_14530"
     CDS             10413..11762
                     /gene="hisS"
                     /locus_tag="VMZ53_14530"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HUS29716.1"
                     /translation="MPVQPRTTQGVLELLPAQQIAFQRMLDTIRRNFELFGFLPVETP
                     VFELTDVLLTKSGGETEKQVYFVQSTGSLKQGDTPELALRFDLTVPLARYVAQHEHQL
                     NFPFRRYQIQRVYRGERAQKGRFREFYQCDIDVIGKDTLPLAYDAEVPALIHSIFKQL
                     DFGPFTIFINNRKLLRGLLESLGIAIDKHPDVLNQVDRLGKQEREQIEAKITEHCDAE
                     TSKRLLDLLTMHPGDSRSAIAALHTFEPANANFKEGLRELEDVYNRTLALGVPPAALQ
                     INLSIARGLDYYTGTIYETFLTDHPKLGSICSGGRYADLAGFYTKSKLPGVGISIGAT
                     RLFSQLLELNLVKVDRPSTAQVVILNVEPALAEDYLHMAASLRSAGINTEVYGGDDKL
                     GKQMKYADRSGAPIGVLVGSRERDANQVKLKLFRENREIDVPRSELLGQIQKLLTPA"
     gene            complement(11759..>12292)
                     /locus_tag="VMZ53_14535"
     CDS             complement(11759..>12292)
                     /locus_tag="VMZ53_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29717.1"
                     /translation="SLADGTVRRFDVPAGPARDQHLGMHWAPTSDAVIVDYDTSASLD
                     IVTGALTPLAKADEMQWLERRSPGLACQTKGFVLEERQQNHEQQIVLLPTASTTNPEQ
                     LSSLETRVLVRATDKSGHSGDGAINLGKKNPEPLSLDAVLPSCEHFLFSLEGKTFVGN
                     IATGKIAFVAFGWGAVL"
BASE COUNT         2197 a         4166 c         3917 g         2012 t
ORIGIN      
        1 aggcgtgccc aacggccagt ggatcgccga cgctttctcg cccggctaca tctcgcccgg
       61 tggcgtcgag ctgacagcgg ggcagggcat cgtggagctt gccatggcgc gcggcgcgac
      121 gatcgaaggc cgtgtcctcg acggagaagg ccgcgggatc gagaacgcga cggttcgcgc
      181 gatcacggca ggccagaacc cgacggagat cagcgcgctc gtcgagcaag accagctgcg
      241 ccggttctcg ggccgcaccg ccgcaccggc cgctgtctcg acgaccaaca gtttcaacgg
      301 tgatccagac ttcatcccgc gcggcgagct cggcgtcacc gtcggaccca ttccgccgat
      361 tccgccacct ggcgtcgttg ccgcgcgtcc cgcatcggtg atcgatccca aggctgcgag
      421 cgcgtcgctc gtgggggagc caccaccgct cgccgtcgat ccggatcgcg catcgatctg
      481 gactacaggt cgcgacggcc gctaccgcat ccggggcctc tcgaagacca agctcagcgt
      541 gctcgccgcg gcagccggct acgccgaagc gcgcagcaag gacgtctcga tcggtaacgg
      601 ccaagtcgtc cttggcgtcg acatcatcct caccgccggc acattcatct tcggcaaggt
      661 cgccgacaaa cgcggcgctc cgatcgtcgg cgcgcagatc accgcgcagc cagagctcgg
      721 cgcaccgctc gatgcattca gcgacgccga cggcatgtat cgcctcggcc cgatcagcgg
      781 caagatcgat cttggagcga acagctacgg cttcgtcgag acgcatcgcc gcgtcgacct
      841 cgcgccggct aaaggccgcc tcgccgccga gcaacgcgaa gacatcacgc tcgaggtcgc
      901 agatgcggtc ctcgccggca cgctcgacga cgcggccggc gcacccatcc ccggcgcaaa
      961 catcgaagtc atcagcggtc cgggatccgg acgtcacgcc gtcgtcgcag cggacggcac
     1021 gttcaacatc gacatgctac cggcgggtcc gctgcgcctc cgcatcgacc atccgcagta
     1081 cccgagcgac gagctcgacg cgacggcctc gaccaccggc cagcgtgtcc gcttgcgcct
     1141 cgccctcggt ggccaggtcg aaggcgtgct gctcgacagc gcgaccggta agccgctcgg
     1201 cgggatgacg ctcgacgcgc gcggtcccgc aggtcggacg gccgatacga cgagcgacga
     1261 caagggcctc ttcaagctcg gcccgctgaa gcccggcaaa tggaagctcg acatcaagct
     1321 acccggctat ctcgcgcaga cccgcgacgt cgacgttccg gtctctcgcg cgccgggcga
     1381 aaccagcgtg cgtgacatcc gcatcgagct gcagcgcggc gcgctggtcg gcggcactgt
     1441 ccgcgatcgt cggggccaac gcgtcgcggg cgcgaaggtc accgtgcgtc gcatcgacgg
     1501 tacgggcgag cccgtcgaag aggacacgaa cgcgcaaggc gagttcctga tccacgacgc
     1561 accgacaggc gagctgatgg tcgccgcgca atccggcgat gccggtggct cgacgcacgt
     1621 cacggtgcgg cccggcgcgg aagtgctgag tctgacgatc gagatccgct agacgtccgt
     1681 cggacattcg gtggacgtcc aaggtgtccc gggacatcgc caaaggttgc taaccgcgcg
     1741 accgcacgcg cacgaacggt ggcctgcgtc gtgctgagca cacgcgcatg caactacgaa
     1801 cactcttgct agtcgccgca gccgcatgtg gcagcgtcga acacaaaccg cccgaggtcg
     1861 atgcgcctgc atcgatcgac tcgcgaatcg atacgccgga tgcagcagca tcgacatcgc
     1921 cgggccgcat cgcctcgacc agcggcggag gcacgaccgc cagcgcacaa caccgcctgc
     1981 gcattcgcat cggtgcacca cccagctaca ggagataagc catgcgaagc ctcatctttg
     2041 tcgcagtatt cctcgccagc acgaccgcaa tcgccggcgt gcctacgcgc atgcccgtgc
     2101 agggttacct caccgacacg aacggaactg tcgtcgatgc gtcggtgcaa attcaatttc
     2161 agatctacgc caccgcgacc ggcggcacgg cgctgtacag cgagaaccag gcggtgaccg
     2221 tcgatgacgg caacctgacg gtctacctcg gcagcaacgt tccgctggac atgtctctct
     2281 tccgcggtgg caccgcgtat ctcggcatca cgctgcaggg cgaggccgaa atgtcgccgc
     2341 gcctcgagct cgccaccgtg ccgttcgcag cggtcgccca gtacgccgaa gagacagcgt
     2401 cggtgccgac cggcgcggtg atgatgttcg acctcgatac gtgcccggcg ggttggaccg
     2461 ccctcgatgc agcgcgcggg cgagcgatcg tcggcgtacc ggccggcggc acgcgcggag
     2521 gaacggtcgg cacggcgctt tcagatgtcg aagatcgcgc gcactcgcat tcggtcggac
     2581 cgttctcgac gacgacttca tcggcgggca accacgtgca caacgttcca ctgagcttca
     2641 gcaccaatca atcgacgcta gacctcacgc atgtacacac cgtcgatcca ccgccgcacc
     2701 agcaccggtg ggcctcgtac ttcatcgatc cgacttcttt gaactcggtg tttcacacgt
     2761 acgccgcgaa cgacggctcg acgaccggcg ccattactct tcaagattgg gacaacggga
     2821 tggacagcgc gggcgctggc tattacccag tcgccgcgcc ggcggcggcc gcgacaggct
     2881 cgacgagctt ctattacacg cagacgacgg atatggggtc gatgaccagc tcgacggcga
     2941 acccaccgtc gacgcacgcg caccaggtca gcagcaacgc gaccgcgcag ctgggcggca
     3001 cgcatatgca tgacgtggcg attgccgcga gcgcgtccgg cggtgcgacg accggcgccg
     3061 tcatgccgta cctgcagctg ctgatctgcc ggaagaacta gccgcggccg cgctggcgac
     3121 gcatccgatt gatgtcgatg atcatcgtcg gcgcggcgtg cagctcttcg tcgtagatcg
     3181 gcttcacgcc actgggcttg cgcgcttgcg gcgggaccgg cggcggttgc gtgcgaccgc
     3241 gcggcgtcgg ctggccacgc gccgtggact gctgctggcg cttcggcggc ggcgcctcgg
     3301 ggacggcgcg acgacctcgg ctgtcgagga tcatcgtctg ctgctggtgg atgtcctcgc
     3361 cgagcgagtc gatgcggcgg ccgtcttgat cgaccttggg catcgtcgtg aagttcgagt
     3421 cgccggagaa atcgttcgat ggcgcgagcg cgacggagac cgaggttggc ttgggctgga
     3481 acggaacctc gggcggctcg aagtcttcgt cgcgcagcat gagcgattcg ccgacgtcgt
     3541 cggacggctc gtcggccatg acgaactctt cgagcatgcg cagctcgcgc tcgatctcgg
     3601 tgttgaacat cttgcggatg aaccgaccga ggtggctgcg cgagtagttc ggcatgaact
     3661 tgcgcaggaa cgcgcgcagg tcgccttcgg cttcggcagc tgtctggtag cggttcgcgc
     3721 ggctgcgcgt caggcacttg tcggcgatct gctcgagctc gggcgggatg cccggttgca
     3781 gctcggcgat cggccggtac ttcgcgtcgc gaactttgat gagcaggtcc atctcgttcg
     3841 tcgcggtgaa cggcgggatg cgcgtcagca tctcgtagag acagctgccg agcgaaaaca
     3901 catcagagcg atggtcgagc ttgcggccga gcgcctgctc ggggctcatg tacttgacct
     3961 tgcccttgat gacgccggtc ttcgtcgtaa cgcgcgacag cgtggccttc gcgatgccaa
     4021 agtcgcacag cttcacttcg ccggcgtagc tgcagatgat gttcgacggc gagacgtcgc
     4081 gatggatgat gcgcagtggg cggccgcgcg agtcgacggc ggagtgagca gcgtgcagcg
     4141 cgccaccgac ttcggcgcag atatacgcgg cgtgctcggg tggtatcggc ttcttcttcg
     4201 cccgacagcg ctcgaggacg gtgcgcatgt ccttgccgtc gacgtgctcc atcgcgatga
     4261 agtactcgcc gtgcgcgcgc gcgaactcgt agacgcgagc gatcgattgg tggcgcagca
     4321 ccgacgcgat cttcgcttcg tcgacgagca tctggatgaa gtcgtcgtct tccgcgaggt
     4381 gcgcgagcac gcgcttgacg gcgacgagat gcggcgtccc gttcgcgtcg aacgtcttcg
     4441 cacgatagat ctcggccata ccgccgaacg cgatgcggtc gagcagatgg taacggccga
     4501 gctgctgagc ccttctcacc ggcgtcgacc agcgtaccat ggcgtggcgt aaccgccgag
     4561 atccgctatg ctacctggca tggttcgcgc gctcggtatc tcactcctgc tgatctccgc
     4621 ctgcgggaag tctgcgagta aacaggacaa cacgaaggcg accacgccaa gtggcgagga
     4681 agtcatccag gtaccgggct ccaaaggtgg gcctgccgtc ggtgccgtcg agggcgcagg
     4741 tcctgacgac ggcaacgtcc aggcgaccgg cgctggcgca cccccgaatc cagcattctt
     4801 cctcaagccc gaagagggca cgctgaccgt cgacaaggcg gaaggcaaag ccggcgcaga
     4861 gtcgaccgcg aagctcagcg tcgcgcccgc gaagggctat cacatggcga ccgactatcc
     4921 gatcaagctc gagctcgagc cgaccgcggg agtcacgctg caaaaggcga agctcgaagc
     4981 aggtggtcgc aacaaagtgc aaggcgacgc cgaaacactc tcggagcaat cactcgcgtt
     5041 cgcggtgaag gcgactgccg agaaggccgg cacgtacgag atcaagggca ccttcaagtt
     5101 cggcatctgc gagaaggaca gctgccatcc gaagaagcag cctgtgacga tcacggtggc
     5161 tgcgaactag cgtactgtcg cgacgcatgg cggaccttcc tccgaccgtc aaggacacgc
     5221 tcgtcggcct gcaccaatcg gtgcgcgagg acttcactcg caaccgccgc gtgctgtcat
     5281 tcagcgagta cctcgcactc gcattcgccg agccggctcg ccagctgcgt agctcgacgc
     5341 agtacatcgt cgattgcttc gaccactacg gcgccgagaa ggtgaagtat ccgtggggcg
     5401 aggtcacgcg cttcaagctg ttcgacgctc cgtgggccgg cggcgaggat cgcctgtttg
     5461 gccaagagca ggtccagaac acgacgtatc ggatcctgcg cacgttcgtt caggacggcc
     5521 ggccgaacaa gctcatcctg ctgcacggac cgaacggctc ggcgaagtcg acgttcatcc
     5581 gctgcctcgg ccgcgcactc gagcactact cgacgctcga cgaaggtgcg ctctatcgct
     5641 tcagctggat cttcccggcg cagaagagca cgaagggcgg cattggcttt ggcggcggcg
     5701 ggcaatcgat cgacacgctc gactcgttcg cgtatctgcc cgacgatcag atcgacgcgc
     5761 gcctcggcga cgagctccgc gatcatccgc tgctcctcgt gccattcccc gagcggcaga
     5821 agctcatcgg caagctgctc gacggcaagg cgttccatct cagcgattac ctgcgtggcg
     5881 gagcgctctc gccgcgcaat cgcgcgatct acgacgcgct gctcgcgagc taccagggcg
     5941 actacgtgca ggtgcttcgg cacgttcgca tcgagcgctt cgaggtctcg catcgctatc
     6001 gccaggcctg ggtgaccgtc gagccgcagc tgtccgtcga cgccagcgag cgtcagatca
     6061 ccgccgaccg cacgcttgcc gcgctaccgc cgtcgctgca gacggtgtcg ctgcacgagt
     6121 acggcggcga agtcgtcggc gccaatcgcg gcatgatcga gttcgacgat ctgctgaagc
     6181 gtccgctcga gcactacaag tatctgctca ccaccgtcga gcgcgcggcc ctgtcgatgc
     6241 agagcgcgac gctgttcctc gacctctgct tcctcggcag ctgcaacgac atccatctgt
     6301 cggcgttccg cgagatcccc gacttccaga gcttcaaggg ccgcatcgag ctcgtgcgcg
     6361 tgccgttcat cctcgacgtc aatcacgagg aagcgctgta ccaagagcgg ctgcaggaat
     6421 cggccggtgg gcgccacatc gctccgcaca ccgcctacgt cgccgcgcta tgggccgtgc
     6481 tctctcgtat gcgcaagccg cagatcgagc ggttcccgtc gacgctcgcc gaagtgatcg
     6541 gccgtctcac gccgctcgat aaggtcgagc tctatacgtc gggcgaagtc cccgaggacc
     6601 tgtctcccga tcgcgcgcgc gagctccacg ctcacatcaa agacctgttc cacgagagcg
     6661 acacgtatcc gatctacgag ggtcgcgtcg gcgcgtcacc gcgcgagatg cagacggtgc
     6721 tgctcgcggc cgcggcgtcg cctcgctacg agtacgtctc gccgctcgtc gtgctcgacg
     6781 agatccagga gctctgcaag caagcgagcc tctacgagtt cctgcgccag gacgtgcagc
     6841 ccggtggcta tcacgaccac aagaagctcg ccgacgtcgc gaagggtcgc ctcttcgatg
     6901 tcatcgacga cgaagtgcgc gtcgccgtcg gcctcgtcga ggagagcgag tacacgcgcg
     6961 tattcgagcg ctacgtgacg cacgtgatgc actcgatcaa gaaagagaaa gtgcgcaaca
     7021 cgtcgacggg acgccaagag gatcccgacg agaacatgat gcgcgaggtc gagcgcaccc
     7081 tcgagattcc aggtcgcgcg gaagacttcc ggcagagcct gatcgcgaag atcggcgcat
     7141 ggtcactcga ccacaaaggc cagaagccgg tgatggccga catcttcgcc gacctcctcc
     7201 gcaagctgcg cgacgcctac tacgagcgtc acaagaaggt catcgcgaag ggcatcacgg
     7261 acctcgtcgt tcttctgtcc ggtaacgagg gtcacctttc gagcgccgac cgtacgcgcg
     7321 ccgatgccgc gttggcgacg atgattaccc gctacgggta cacccgggag tcggcgcggg
     7381 atctggtcgg ggcactcgcc tcgatgcgct atcgctgacc cgtcgaactg ttgcgcctac
     7441 atcgcgggcg ctcgtatcct cgaggatggt catgttgcga gggatcgtct gcatttgcgt
     7501 gctcgccgtc gccggcgtgg ccgctgccga cgcaccacgc atgctgaagg gtccctacct
     7561 ccaggacctc gcgccgacgt cgatcaccgt gatgtggcag atggagccgc agcaggcagc
     7621 gacgctcgtt gtcgaagggc cgggcggtac gcgcacgatc gacgtcaagg cggatcgcgt
     7681 cgctgaagcg cagatcgctg ggctcacgcc gtcgagccgt tatcgctatc gcgtcatcgc
     7741 gggcgatcag caatggcagg gcgagttcgc gaccgcgccg ccgatcggca aggatgtccc
     7801 gttctcgttc gtcgtgttcg gcgacagccg ttacggcgaa gcgcaccacc gccgcgtcgt
     7861 caaccgcatg agccaagagg tgccggactt catgctcggc acgggcgaca tggtcgacga
     7921 gggctatcgc caggagcagt ggcaatcgtt cttcgacatc gagaacgaga tgctgcggga
     7981 caacgtctac ttcccggcac tcggcaatca cgatcgccag ggcaggggcc gcaccgcaga
     8041 ttcgtatcgc gcgttcttct cggtccccga gaacggcaac gagaccgagc gctactacgc
     8101 attcacgtac gcgacatcgc ggttcctcgt cctcgactcg aatctctact cgttcgcgct
     8161 caccgaccag acgtcgtggc tcgagcgcga gctcatcgcc gcgcgccagg atccggcgat
     8221 ccatcacatc ttcgtcgtga tgcaccaccc gccgttctcg atctcgctgc acggcggcgc
     8281 ggtcgatctg cgcgagcggt ggacgccgct gttcgagcaa tacaacgtga cggccgtctt
     8341 cagcggccac gaccacgtgt acacgcgcgc gatgcacaac ggtctccact acttcatcag
     8401 tggcggcggc ggcgcaccgc tctatccacg ccgcccgaag tcgaatccga tcgacgtcgc
     8461 cgccgtgaag aagttcgagc gcgtgctgca ctacctgcgc gtgacgatca cgggcaatcg
     8521 cgtcgaggtc accgcggtgc gcgccgacgg aacgacgatc gagacgacct cgtggaagga
     8581 cgggcccgag ccgaccctcg agcagccgaa actgtcggcc acgggcgctc cgcctgcggc
     8641 tgcagcgact gccgctccca cggccccgcg cgcgaaggtg ccgccgcccg acgacggcaa
     8701 catgctctgg atcgggctcg gcggcctcgt cgtcgtgctc ggcgccgcgg tgttcgtcgt
     8761 ccgatcgctc cgctgatcag cgatactggt cgcgatgcgg cgcttggtgc tcctctgtct
     8821 cgtcggttgt ggcggcgagc cacccgagtg cggcacgagc ctcgccgagg cgatcttcgt
     8881 cgagaccgcg ctcgatgcgt acgaccacgc attcggcccg acggcgcagc taaccgccac
     8941 gtgtccaggc ggcgggacgt cgacggtcga aggcgcatcg gggccgagcg cgacagcgcc
     9001 gcgcgtcgac gactacaagg tgacgtacgc tggctgcgcg acgctcgatg cgaggctcac
     9061 actcgacggc gtcgtcgaga ttcgcggcac gtgggtcggc ggcacggacg ccgcgtactg
     9121 cgacgaatgc acgatcacgt cgagcgcgct gatgatccgc ggcaccgagg accgatgcga
     9181 cgactcgcac atcgcccgga cgtgcccggt cgacttcaag gtgaagggtt cggcgtcaaa
     9241 tgtgccgcag cggtacgagg gctcgatctg cgacttcgat ttcccgtaga ctggggatca
     9301 tgcgcaggtg tgtgggagtt ggaacggtcc tgttctccgt catggttgca gtcgcgtgcg
     9361 ccgaacccga ggaaccggaa tgcacgtcga gcatcgcgtt cgacatccat tacctctacg
     9421 tgcaggcaac gaatgccacg acgcgctcgg gcggctccgg ttcgaactgg acgtgtccgc
     9481 agggcggttc ggcgacggtg accgagacgt cgacgacgaa caacgcaacg cagacgaatg
     9541 attcgcagct gacgttccac atgtgcagct acgtcatgaa cggcttcgac atccgcctcg
     9601 atggcccatt gcacgcgcac aacgtgagca acattgtcgg caacctcgtc tactgcaata
     9661 cgtgcgagct cacgtcgccg ggcctcaaga tctacggcag cgagcttctc tgcaatgcgg
     9721 atcctatcga cgcgacgtgc gaagtctcgt tcacgatcaa aaacgagagc acgcaatcga
     9781 tgggcgacta cgccggctcg atctgctcgc tcacattccc ctgatcgcag ccgcgaggtt
     9841 cgcgcgcgct tgcgcgtcga gacgcgagtg gaagtactcg gtgatgaaga tgcgcggcag
     9901 cttcgcgagg cccgcgagaa acgccttgcg ccccgcgcga aacgcatcgg gcggcacgtg
     9961 gctgtactcg cgcgcgatcg ccgcgtcgta ggctgcgaac tgctccgccg gcgccccgac
    10021 gatcgccatg tcggcatcga ggaagtgcgc cgcgtcgtga ccggcatcgc cgtgcgaggt
    10081 gtggctcatc gtcatcagga tcagctgcgc gacgtcgtcg ccaaaacccg ctcgccgcgc
    10141 ccaatccgcc gagcgcgcct cgttgtcctt ggccccgggc acgtaaatcg cgtcgtgaaa
    10201 cacgatcgct gcatagacgg cggccgggtc cttccacccg atgtcatcgg cgacgcgatc
    10261 gaaccacccg agcagctccg cgatatgcgt caggttgtga tacgcgcgat gcggctcgct
    10321 gtacgccgca accaattcac gcgcgacatc gtcgggaagg tgcactggct ggtggcggca
    10381 ctcgaacacg aggctatagt gccgtgcttc ccatgccagt acagccccgc accacgcaag
    10441 gagtcctcga gctcctgccg gcgcagcaga tcgcgttcca gcggatgctc gatacgatcc
    10501 gccgcaactt cgagctcttc ggcttcctgc ccgtcgagac gcccgtcttc gagctcaccg
    10561 acgtcctcct cacgaagtcc ggcggcgaaa cggagaagca agtctacttc gtccagtcca
    10621 ccggctcgct caagcaaggc gacacccccg agctcgcgct ccgcttcgac ctcaccgtgc
    10681 cgctcgcgcg ctacgtcgcg cagcacgagc accagctcaa cttcccgttc cgtcgctacc
    10741 aaatccaacg cgtctatcgc ggcgagcgtg cgcaaaaggg ccgcttccgc gagttctatc
    10801 aatgcgacat cgacgtgatc ggcaaggaca cgctgccgct cgcgtacgac gccgaagtcc
    10861 ccgcactcat ccactcgatc ttcaagcagc tggacttcgg cccgttcacg atcttcatca
    10921 acaaccgcaa gctgctgcgc ggcctgctcg aatcgctcgg catcgcgatc gacaaacacc
    10981 ccgacgtcct caaccaagtc gatcgcctcg gcaagcaaga gcgcgagcaa atcgaagcca
    11041 agatcaccga gcactgcgac gccgaaacgt cgaagcgcct gctcgatctg ctcacgatgc
    11101 acccaggcga ttcgcgctcc gcgatcgccg cgctccacac gttcgagccg gccaacgcga
    11161 acttcaaaga gggcctgcgc gagctcgagg acgtctacaa ccgcacgctc gcgctcggcg
    11221 tcccgcccgc ggccctccaa atcaatctct cgatcgcacg cggcctcgat tactacaccg
    11281 gcacgatcta cgagacgttc ctgaccgacc accccaagct cggctcgatc tgctccggcg
    11341 gccgttacgc cgacctcgcg ggcttctaca cgaagagtaa actgcccggc gtcggcatct
    11401 cgatcggcgc gacgcgcctc ttcagccaac tcctcgagct gaacctcgtc aaagtcgatc
    11461 gcccgtccac cgcgcaggtc gtgatcctca acgtcgagcc ggcgctcgcc gaggactacc
    11521 tgcacatggc agcctcgctc cgcagtgccg gcatcaacac cgaggtctac ggcggtgacg
    11581 acaagctcgg caagcagatg aagtatgccg accggtcggg cgccccgatc ggtgtcctcg
    11641 tcggctcgcg cgagcgcgac gcaaaccagg tcaagctgaa gctgttccgc gagaaccgcg
    11701 agatcgatgt tccgcgcagc gagctgctcg gccagatcca aaagctgctc acacccgcct
    11761 agagaacggc gccccagccg aaggcgacaa acgcgatctt tccggtcgcg atgttgccga
    11821 cgaacgtctt gccctcgagg ctgaagagga agtgctcgca cgatggcagc acggcgtcga
    11881 gcgagagcgg ctctgggttc ttcttgccga ggttgatcgc gccgtcgccg ctgtggcctg
    11941 acttgtcggt ggcacgcacg aggacgcggg tctcgagcga tgacagctgc tcggggttcg
    12001 ttgtcgaagc cgtgggtaac agcacgatct gttgctcgtg gttctgttgg cgctcttcca
    12061 gcacgaagcc ttttgtctgg caggcgaggc caggcgacct gcgttcgagc cactgcatct
    12121 cgtcggcctt ggcgagagga gtgagcgcgc cggtgacgat gtcgaggctc gcggacgtgt
    12181 cgtagtcgac gatcacggcg tcgctcgtcg gggcccagtg catgccgagg tgctgatcgc
    12241 gtgcggggcc ggccgggacg tcgaagcgac gcacggtacc gtcggcgaga ct
//
LOCUS       DATLBZ010000275         4718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1792286, whole genome shotgun sequence.
ACCESSION   DATLBZ010000275 DATLBZ010000000
VERSION     DATLBZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792286"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..525
                     /locus_tag="VMZ53_14540"
     CDS             352..525
                     /locus_tag="VMZ53_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29718.1"
                     /translation="MTTRSSSPRLDTIVTRQRSSRIRDIAFALLVVAAGAVSLSTVSY
                     AAHAANQSDVAKR"
     gene            559..864
                     /locus_tag="VMZ53_14545"
     CDS             559..864
                     /locus_tag="VMZ53_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29719.1"
                     /translation="MWRACVALTISGCSLISVRVPDQRQPDRECTYKMPIIDTVFAVS
                     AIAGDLVYVATSKPQGGFVDMRPVFIAIPLIVAGVDAASAIYGFVEERRCYRLPPAA"
     gene            complement(883..2157)
                     /locus_tag="VMZ53_14550"
     CDS             complement(883..2157)
                     /locus_tag="VMZ53_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HUS29720.1"
                     /translation="MTATPDEETAIAYLRSPLAIRARCENVLDAALADKLAHFSIDLS
                     ALPAVVDEVVAVTRQQYPSLQIPVHGRINHFRAGGVDRVARLDRRMQALPKDERSRAW
                     IDLVLVSVLLDAGAGNQWRYREAASNLELGRSEGLAVASLHAFEVGMFSADPSQPFRV
                     DAAALRALEPRALAQAFQVSDKNPLVGIEGRIGLIRALGGALTDAPTIFPGERPGGIL
                     DHLIWIAAEKRSPGGSVVPDMSMQIDAPRILAALLEGLGPIWPGRIELAGTNLGDVWR
                     HPYAGGTGPSAGLVPFHKLSQWLAYSLFEPLQRAGHMIAQPGALTGLPEYRNGGLLVD
                     LGVLVPKHDAVRAEVHAPGDEVIVEWRALTVALLDRVAEKVREKLGKSPTELPLANIL
                     EGGTWAAGRAVAARLRPDGGSPIQIASDGTVF"
     gene            complement(2169..2738)
                     /locus_tag="VMZ53_14555"
     CDS             complement(2169..2738)
                     /locus_tag="VMZ53_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29721.1"
                     /translation="MRRAILGALMLGGCLYNPSVDVDKQAVTMPQGTHEDVVISLDGE
                     AINDFDGVYWVVDDPAMITVAPAWDGKHLTIGGNLPGVTRVHVNSHGQTFDIEVQVGP
                     PAIVKMWIEPEVVQASVGGDVQVKAIAVDTTFHIVDISHESRWTIRNPDLASLDMNGM
                     MLHASAQGQTTLHAVNGGVATVVPITILK"
     gene            complement(2811..4064)
                     /locus_tag="VMZ53_14560"
     CDS             complement(2811..4064)
                     /locus_tag="VMZ53_14560"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HUS29722.1"
                     /translation="MSDKVKHKSKHIKLTSHPGSTKPIPITWGAGDPQTRGPIIATLT
                     TPEHRNVIGTHSGSYAVYRALAIAAKALDPQHVPDLTNTAPTDPVGPYPSWHDPERIV
                     SIDPYGHIVQDVFLDYLKKGYDIRPTIAVTKAHIDMPEIVDAMEAGRIPIDGKIVKSK
                     REVPVTKAATEPVWYLPGVAKRFGCTESELRRTLFEQTAGMFPELVTRGDLDVFLPPI
                     GGQTIYMFGDPKKLADPNVTLTARVHDECNGSDVFGSDICTCRPYLAHAIEECIRGAQ
                     AGGVGLIVYFRKEGRALGEVTKFLVYNARKRQQGGDSAAQYFARTECVAGVQDMRFQE
                     LMPDVLHWLGVKKIHRLVSMSNMKYDAIVRAGIEVGERVKIPEELIPADAKVEMDAKR
                     AAGYFTPDAPLAPEELSKAKGRSLE"
     gene            4180..>4718
                     /locus_tag="VMZ53_14565"
     CDS             4180..>4718
                     /locus_tag="VMZ53_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29723.1"
                     /translation="MKQAVIALALASLLCACTTLGPNPATTGVSAIPAGRPSLDAQVG
                     TVPAFYASQSAQNEAKGAAVAQAGFLLEPDRYLKLPGLVIGGRLYGQSGDTLGEPYVG
                     YRANIDESIAVGGGVYGSAKRSTKQLASYHGTRVGAEAGIDAELSSLTSWLTLHTQAS
                     LAATRIMTSGTYCVDPMGIA"
BASE COUNT          793 a         1507 c         1564 g          854 t
ORIGIN      
        1 gcgtgcgcga cctgcgccgt cgtcgtcacg gtgacgaacg agaccgtcgc gacgagagcg
       61 acgcacgcgg cgaagaatgc gtcgcggacg cggttcgagt tgcgggcggc gatcgtcgag
      121 aggcggttgg aggttgcttt cggcatgtcg acctctactg cgaccggcgg gccagtccag
      181 agcggccgaa ttccaggcac ttgcgggggc tgacgcggcg ttgccctgtc gcaggcgctg
      241 tcgcagggac cgtcacactg gcgatgagaa cgagacaagt gaccgcagct gttcgagcga
      301 ctcatggcat cgcagttgaa gaaggaacga aacgccggaa cggaggcttt catgaccacc
      361 cgatcttctt ccccgcgtct cgacaccatc gtcactcgtc aacgcagctc gcgcattcgc
      421 gacatcgcat tcgcactgct cgtcgtcgcc gcaggcgccg tctcgctgtc gactgtctcg
      481 tacgcagcac acgccgcgaa ccagagcgac gtcgccaagc gctagcccga cccgcgcagt
      541 agacgctaca ttcaagctgt gtggcgagcg tgtgtggcgt tgacgatcag cggttgctcg
      601 ctcatcagcg ttcgggtgcc tgaccaaaga caacccgacc gcgagtgcac gtacaagatg
      661 ccgatcatcg atacggtatt cgccgttagc gccatcgccg gcgacctcgt gtacgtcgcg
      721 acgagcaagc ctcagggtgg cttcgtcgac atgcgtcccg tgttcatcgc gatcccgctg
      781 atcgtcgctg gcgttgatgc ggcgtcggcg atctacggct tcgtcgaaga acgccgttgc
      841 taccggttgc cgcccgccgc ttagaagccg gtgccgtcga gatcagaaga cggttccgtc
      901 ggaggcgatc tgaattggag aaccgccgtc gggacgaagc cgtgcagcga ctgcgcgtcc
      961 tgcggcccac gttccgcctt cgagaatgtt cgcgagcggc agctccgtcg gtgacttgcc
     1021 gagcttttcg cgaacttttt cggcgactcg atcgaggagc gcgaccgtca acgctcgcca
     1081 ctcgacgatg acttcgtcgc ctggcgcgtg gacctcggcg cgaacagcgt cgtgcttcgg
     1141 cacgagcacg ccgagatcga cgagcaagcc accgttgcgg tattcgggca gacccgtgag
     1201 cgcgcctggt tgggcgatca tgtgaccggc gcgttgcagc ggttcgaaca acgagtacgc
     1261 gagccactgg ctcagcttgt gaaacggcac gagaccagcc gagggtccgg tgccgcctgc
     1321 atacggatga cgccacacgt cgccgagatt cgtgcctgcg agctcgatgc gtcccggcca
     1381 gatcgggccg aggccctcga ggagcgcggc gagaatgcgt ggcgcatcga tctgcatcga
     1441 catgtcgggc acgaccgagc cgccgggcga gcgtttctcg gccgcgatcc agatgagatg
     1501 atcgaggatg ccgcccgggc gttcaccggg gaagatcgtc ggtgcgtcgg tgagtgcgcc
     1561 gccgagcgcg cggatgagac cgatgcgccc ctcgatgccg acgagcggat tcttgtcgga
     1621 gacctgaaat gcttgggcga gtgcgcgcgg ctcgagcgca cgaagtgccg cggcgtcgac
     1681 gcgaaatggc tgtgacggat cggccgagaa catgccgacc tcgaacgcgt gcagacttgc
     1741 gacggcgaga ccttccgagc ggccgagctc gaggttgctc gcagcttcgc gatagcgcca
     1801 ctggttgccg gcgcccgcgt cgaggaggac ggacacgagg acgagatcga tccacgcgcg
     1861 cgaacgctcg tctttaggca gcgcttgcat gcggcgatcg agacgcgcga cgcgatccac
     1921 gccgccggcg cggaagtggt tgatgcgccc gtgcacggga atctgcagcg acgggtattg
     1981 ctgtcgcgtc actgccacga cctcgtcgac gaccgccggc agcgccgaga gatcgatcga
     2041 gaagtgcgcg agcttgtcgg cgagcgccgc gtcgagaacg ttttcgcagc gcgcgcgaat
     2101 ggcgaggggc gaccgcaggt acgcgatcgc ggtttcctcg tcgggcgtgg ccgtcatcgg
     2161 ccgcaaggct acttcaagat cgtgatcggg actaccgttg ctaccccacc gttcacggca
     2221 tgcaaggttg tctgaccctg cgccgacgcg tggagcatca tgccgttcat gtcgaggctc
     2281 gcgaggtcgg gattgcggat ggtccaccgg ctctcgtgcg agatgtcgac gatgtgaaac
     2341 gtcgtgtcga cggcaatcgc cttcacttgg acgtcgccgc cgaccgatgc ctgcaccacc
     2401 tcgggctcga tccacatctt cacgatcgcg ggcggcccga cctgcacctc gatgtcgaag
     2461 gtctggccgt gcgagttgac gtggacgcgc gtgacgcccg gaaggttgcc gccgatggtg
     2521 aggtgcttgc cgtcccacgc cggagccacc gtgatcatcg ccggatcgtc gacgacccag
     2581 tacacgccat cgaagtcgtt gatcgcctcg ccatcgaggg agatgacgac atcctcgtgc
     2641 gtgccctgcg gcatcgtcac cgcctgtttg tcgacgtcga cgctcgggtt gtagaggcag
     2701 ccacccagca tcagcgcacc aagaattgct cgtcgcattc ctcggtcgta accgaatacc
     2761 tacatgtggg cgacttatcg gcagcgagcc atgtcgctta ataagtgcgg ctactcgagc
     2821 gagcggcctt tggccttcga cagctcttcc ggtgccagcg gcgcgtccgg tgtgaagtag
     2881 ccagcggccc gcttcgcgtc catctcgacc ttcgcgtccg cgggaatgag ctcttcggga
     2941 atcttcacgc gctcgccgac ctcgatgccc gcgcgcacga tcgcgtcgta cttcatgttc
     3001 gacatcgaca cgaggcgatg gatcttcttc acgccgagcc aatgcaggac atccggcatc
     3061 agctcctgga agcgcatgtc ctgcacgccg gcgacgcact cggtgcgagc gaagtactgc
     3121 gccgcggagt cgccaccctg ctgccgcttg cgcgcgttgt agacgaggaa ctttgtcacc
     3181 tcgccgagcg cgcgaccttc tttgcggaag tagacgatga ggccgacgcc gcctgcttgc
     3241 gcgccgcgaa tgcattcctc gatcgcgtgc gcgagatacg gccggcacgt gcagatgtcc
     3301 gagccgaaca cgtctgagcc gttgcactcg tcgtgaacgc gcgcggtgag tgtgacgttc
     3361 ggatcggcga gcttcttcgg atcgccgaac atgtagatcg tctggccacc gatcggcggc
     3421 aggaacacgt cgaggtctcc gcgcgtgacg agctcgggga acatgccggc cgtctgctcg
     3481 aacagcgtgc ggcgcaattc gctctcggtg cagccgaacc gcttcgcgac accgggcaga
     3541 taccagacgg gctcggtcgc cgctttcgtg accggcacct cgcgcttgct cttcacgatc
     3601 ttgccgtcga tcggaatgcg gcccgcctcc atcgcatcga cgatctcggg catgtcgatg
     3661 tgcgccttcg tgaccgcgat cgtcgggcgg atgtcgtagc ccttcttcag gtagtcgagg
     3721 aacacgtcct gcacgatgtg gccgtacgga tcgatcgaga cgatgcgctc tggatcgtgc
     3781 cagctcggat acggaccaac gggatccgtc ggcgcagtgt tcgtgagatc cggaacgtgc
     3841 tgcgggtcga gcgccttcgc tgcgatcgcg agtgcgcgat acacggcgta gctgcccgag
     3901 tgcgttccga tcacgttgcg gtgttcgggg gtcgtgagcg tcgcgatgat cggcccgcgc
     3961 gtctgtggat cgccggcgcc ccacgtgatc gggatgggct tcgtgctccc gggatgtgag
     4021 gtcagcttga tgtgcttgga cttgtgtttg accttgtcgg acatagggcg ctcgccgcgc
     4081 gggcgaggga agtcctcgaa tctagactcc aaatccggtg ccgtccactg ccgggaaatt
     4141 ccagctctca cgccgaggcg ctcgcgtgtt gtctctttca tgaagcaagc cgtcattgct
     4201 ctcgcgctcg cgtctttgct gtgtgcctgc acgacgttgg ggccgaaccc cgccacgacc
     4261 ggtgtatccg cgattcccgc aggaaggccg agcctcgacg cacaagtcgg caccgtcccc
     4321 gcgttctacg cgagtcagtc tgcacagaat gaagcgaagg gcgcagcggt cgcgcaggcc
     4381 ggctttctgc tcgagccgga tcgctacttg aagctgccgg ggctcgtcat cggtggtcgt
     4441 ctgtacggac agtcgggcga cacgctcggc gagccgtacg tcggctatcg cgcgaacatc
     4501 gacgagagca tcgcggtcgg tggtggcgtc tacggcagcg cgaagcgctc gacgaagcaa
     4561 ctcgcgagct atcacggcac gcgcgtcggt gcagaagcgg gcatcgatgc ggagctctcg
     4621 tcgctcacgt cgtggctcac gctccacacg caagcgtcac tcgcagcgac acgcatcatg
     4681 acgagcggca cctactgcgt cgatccgatg ggcatcgc
//
LOCUS       DATLBZ010000276         6198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2537740, whole genome shotgun sequence.
ACCESSION   DATLBZ010000276 DATLBZ010000000
VERSION     DATLBZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537740"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VMZ53_14570"
     CDS             <1..216
                     /locus_tag="VMZ53_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29724.1"
                     /translation="NLVGVLSAGPRVPAIVGNAVLYVDGHPVASVEGGELVMRGPLPP
                     GARVDDDLTYHPPPRPASPAPQAALPL"
     gene            332..736
                     /locus_tag="VMZ53_14575"
     CDS             332..736
                     /locus_tag="VMZ53_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29725.1"
                     /translation="MDPVRHVQALLATWRGRFIVAFLAVQLLAPLTYYIARRDPHDER
                     FAWRMFSPMRMAQCKVAISVDGEPVALYAKFHEAWIEMAQRGRFVVIEEMSSKLCEQN
                     RGKRVEVSLDCTYLDRKPAHYGGQNMCEHPTL"
     gene            747..2465
                     /locus_tag="VMZ53_14580"
     CDS             747..2465
                     /locus_tag="VMZ53_14580"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HUS29726.1"
                     /translation="MAKRRNKGGNKRQSDKRDTRDRGNDKRESDKRASDSDGGIDKRD
                     SDKRASDTRDGGNDKRESDKRPSDTRDGGNDKPSRGKQRREAVRLERAAGAQTSELSS
                     SEPVAEPAFWFGFDISWAKLVLGRFVFFALLAIDSLLNFAHAPRYGAGDFNVAQLPIF
                     DVLAPTRVSYAIGSLLCAWLFALAACGAATRIVLPIATVVYGWLYFGSHLDSYQHHYL
                     VWLIVLLSCFVPWQKPADAVPATRVRSWALRLVLVQLGILYLWAAISKLSPAWFDGRT
                     LGSQIHGGMRSFIDATIGIKGASAMVVFVELALAGTVWNKRTWWLAAPLGLALHLGIV
                     KTNLEIGLFAWLMVGLYIFIIPDRVWLWLASRRPVAATYRFVGAVRESFGGALAWIVL
                     MMSVGLGFVLAFVSRFDYSAVVGLSLVGGVIVLAIFERRHVGRLAAAHLVALTLWVVV
                     DRSSSSAADYYRFWGGSSRRLGDTKTSEYAYRKMTRVAPDDGGGHFQLGKLLLARDAN
                     DEGLAELRLAQQLEPLKSRAYTAEAKWLATHGKKDDAIVKAREATIIDPTDADARSLL
                     DSLGGQ"
     gene            2462..3202
                     /gene="nth"
                     /locus_tag="VMZ53_14585"
     CDS             2462..3202
                     /gene="nth"
                     /locus_tag="VMZ53_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HUS29727.1"
                     /translation="MTAVATDRREDRRESRVQDGPVAPRDRKARAAKILATLESLFPT
                     PAIPLDHQDPFQLLVAVILSAQCTDARVNMVTPALFKRAPDAHAMAKMTAAQIMPFIR
                     TCGLAPAKAKNLAAMARTLVAEHGGTVPNNFDALERLAGVGHKTASVVMAQAFGVPAF
                     PVDTHIHRLAARWQLSRARNVEETERDLKAVFPQDRWNVLHLQIIYFGRQHCPARGHT
                     PASCPICSWAMSKARADREARAQRRTKS"
     gene            3390..4310
                     /locus_tag="VMZ53_14590"
     CDS             3390..4310
                     /locus_tag="VMZ53_14590"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HUS29728.1"
                     /translation="MRRLAVVVAVAVAGCSFGLQGIPKDRKLETTFPKTAAATELPEQ
                     FKVVAFNIHKEPAAKFIAGMQADRATRDADLILLEEVPRFDGQEGCSAACGAGKLLGY
                     YAIYAPAHHAEERDIGVAILSRAPITSARVIELPWNDVHFNAGRRAALAVTIQQAGRP
                     ITVYAVHLENRLSVGDRKKQMTPILEDAKRQPTPVLIAGDFNTNPFHWVSHWLPLPTA
                     GHQATKLESLVRSYGFDTPVKDSGPTHRFIGMKLDAIYTRGFETRNFGTAHAKDVSDH
                     LALWATVNAKRAATKPVAPAANVATVAATK"
     gene            4392..4976
                     /locus_tag="VMZ53_14595"
     CDS             4392..4976
                     /locus_tag="VMZ53_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29729.1"
                     /translation="MRTLTTLGLLAVLTGTAAADHGVYRDRSFSSQQSLRNAPQNRQP
                     QTEERYQERNKLADAGLSPYSNHAVVQLPQAGGSLDYLELRAGRTRVALRDVVVQFAD
                     GSTIHTGSRGVVEPFEGRVIDLPSGCAQVVAVMPEYETPGHGASLEVFGVHEHRTGWG
                     YQPNSYQPNSYQPNGYQPTSYQPTGYQTNGWGYR"
     gene            5056..5868
                     /locus_tag="VMZ53_14600"
     CDS             5056..5868
                     /locus_tag="VMZ53_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUS29730.1"
                     /translation="MTDSQLELISHPLCPFVQRAAALLTECGVPFTQRHVDLQNKPAW
                     FLALSPRGKVPVLRVDGTTIFESNVILEYVAERFAPQLLTGDPLLRARRRMWMTISDD
                     LLMTNYKVAVAATRADRDSAVAAAKETLGRFEPLLSARTFIGVDPAQLSSNDNASNGD
                     NAARASNPDNAAPASNRDNAAPASNPVDAASMATKRATAKDAPGLIDFAAGPGLVRFE
                     KLGRELGIDFYEGLPQVARWSRAIAQRAAFTRTLVADFDARFREFVRHELAA"
     gene            complement(5977..>6198)
                     /locus_tag="VMZ53_14605"
     CDS             complement(5977..>6198)
                     /locus_tag="VMZ53_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29731.1"
                     /translation="AARYVAPFSTNFVILFARLFPTRVWDWAMRQVAYLNAKNLDVTS
                     TSSAKPAESPASAPFSSAKPAVDARASAN"
BASE COUNT         1045 a         2176 c         1952 g         1025 t
ORIGIN      
        1 aacctcgtcg gtgtgctgtc cgccggcccg cgcgtccccg cgatcgtcgg caacgccgtc
       61 ctctacgtcg acggccatcc cgtcgcgtcc gtcgagggcg gcgagctcgt catgcgcggc
      121 ccgctcccac cgggagctcg cgtcgacgat gacctcacgt accacccgcc gcctcgcccc
      181 gcctcgcccg cgccgcaagc cgcgctgccg ctgtagcacg gccgcgggcc gcacctgtct
      241 tctcgcgcat cgccccgccg caagccgcgc tgccgctgta gctcggccgc gcgcctcgca
      301 tcgcgtcgcc ccgccgtgtt agctctgcag tatggatccg gttcgccacg tccaagcgct
      361 tctcgccacg tggcgtgggc gcttcatcgt cgcgttcctc gcggtccagc tcctcgcgcc
      421 tctcacgtat tacatcgcgc gccgcgatcc acacgacgag cgcttcgcat ggcgcatgtt
      481 ctcaccgatg cgcatggcgc aatgcaaagt cgcgatctcc gtcgacggcg agccggtcgc
      541 gctctacgcg aagttccacg aagcgtggat cgagatggcg cagcgcggcc gcttcgtcgt
      601 catcgaggag atgtcctcga agctatgcga gcaaaatcgc ggcaagcgcg tcgaagtctc
      661 gctcgactgc acgtacctcg accgcaagcc cgcgcactac ggcggtcaaa acatgtgcga
      721 gcaccccacg ctgtaacgcg accacgatgg ccaagcgacg caacaaaggc ggcaacaagc
      781 gccagagcga caagcgcgac acgcgcgatc gcggcaacga caaacgcgaa agcgacaagc
      841 gcgcgagcga cagcgacggc ggcatcgaca agcgcgatag cgacaagcgc gcgagcgaca
      901 cgcgcgacgg cggcaacgac aaacgcgaaa gcgacaagcg cccgagcgac acgcgcgacg
      961 gcggcaacga taagccaagc cgtggcaagc agcggcgcga agcagtgcgc ctcgagcgcg
     1021 cggccggtgc gcagacgtcg gagctttcgt cgagcgagcc cgtcgcggag ccggcgttct
     1081 ggttcggctt tgacatctcg tgggcaaagc tcgtgctggg ccgcttcgta ttttttgcgc
     1141 tcctcgcgat cgactcgctg ctgaacttcg cgcacgcccc gcggtacggc gccggcgact
     1201 tcaacgtcgc gcagctgccg atcttcgacg tgctcgcgcc gacacgcgtc tcgtatgcca
     1261 tcggctcgct gctttgcgcc tggctgttcg cgctcgccgc gtgcggagcc gcgacgcgca
     1321 tcgtgctgcc gatcgcgacc gtcgtctatg gctggctgta tttcggcagc cacctcgact
     1381 cgtatcagca ccactacctc gtgtggctca tcgtgctgct gtcgtgcttc gtgccgtggc
     1441 agaagcccgc cgacgccgta cccgcgacgc gcgtgcggtc gtgggcgctg cgcctcgtgc
     1501 tcgtgcagct cggcatcctc tatttgtggg ccgccatctc gaagctgagc cccgcgtggt
     1561 tcgacggccg cacgctcggc agtcagatcc atggcggcat gcgcagcttc atcgatgcga
     1621 cgatcggcat caaaggtgcg agcgcgatgg tcgtgttcgt cgagctggcg cttgcgggca
     1681 ccgtctggaa caagcggacg tggtggctcg cggcgccgct cgggctcgcg ctgcatctcg
     1741 gcatcgtcaa gacgaacttg gagatcgggc tgttcgcgtg gctgatggtc ggcctctaca
     1801 tcttcatcat tccggatcgc gtgtggctgt ggcttgcatc gcgacggcca gtggcggcga
     1861 catatcgatt cgtcggcgcg gtgcgcgaat cgttcggcgg cgcgctcgcg tggatcgtgc
     1921 tcatgatgag cgtgggtctt ggcttcgtgc tcgcgttcgt ctcgcggttc gactacagcg
     1981 cggtcgttgg cctctcgctc gtgggcggcg tcattgtcct cgcgatcttc gagcggcggc
     2041 atgtcggacg cctcgccgcg gcgcacctgg tcgcgctgac gttgtgggtg gtcgtcgatc
     2101 gatcgtcgtc gagcgcggcg gattactacc gcttttgggg cggctcctcg cgtcgcctcg
     2161 gcgatacgaa gacgtcggaa tacgcgtacc gcaagatgac gcgcgtcgcg cccgacgatg
     2221 gcggcggcca cttccagctc ggcaagttgc tgctcgctcg tgacgcgaac gatgaaggcc
     2281 tcgccgagct tcggctcgct cagcagctcg agccactcaa gtcgcgtgcg tacaccgccg
     2341 aggccaagtg gctcgcgacg cacggcaaga aagacgacgc catcgtcaaa gcgcgcgaag
     2401 ccacgattat cgacccgacc gacgccgacg cgcgaagtct gctagacagc ctcgggggac
     2461 aatgactgcc gtcgctacag accgtcgcga agatcgccgc gaatctcgcg tgcaggacgg
     2521 cccggtcgca ccacgcgatc gcaaggcacg cgccgcgaag atcctcgcga cgctcgagtc
     2581 gctgtttccg acgcccgcca ttccgctcga tcatcaggat ccatttcagc tgctcgtggc
     2641 cgtgatcctg tcggcgcagt gcaccgacgc gcgcgtcaac atggtcacgc ccgccctctt
     2701 caagcgcgcg ccggatgcac atgccatggc gaagatgacg gctgcgcaga tcatgccgtt
     2761 catccgcacg tgcggtctcg cccccgccaa ggcgaagaac ctcgccgcga tggcgcgcac
     2821 gctcgtggcg gagcacggcg gcaccgtgcc gaacaacttc gacgcgctcg agcgactggc
     2881 cggcgtcggg cacaaaacgg caagcgtcgt catggcgcag gccttcggcg tgccggcatt
     2941 tcccgtcgac acgcacatcc atcgactcgc cgctcgctgg cagctgtcgc gcgcacgcaa
     3001 cgtcgaggag accgaacgcg acctgaaggc cgtcttcccg caagaccgct ggaacgtcct
     3061 ccacctccag atcatctact tcggtcggca gcactgcccg gcgcggggcc acacgccggc
     3121 gagctgtccg atctgcagct gggcgatgtc gaaggcgcga gccgaccgcg aagcgcgcgc
     3181 gcaacgacgc acgaagtcgt agctgacccg tccgtagctc acgcgccgta gctcacccga
     3241 tcgtgtgccc ccactcgacg ttacgtaccc gagtgggtga ttctcgtcgg cgaattctgc
     3301 atggacggcg atcttcggac gactccggca aattcccgac ccgtttttcc aggcggcgaa
     3361 cccgcccgaa agtgctaagt ctggtgttca tgcgccgtct tgccgttgtc gtcgccgttg
     3421 ctgtcgcggg ttgcagcttc ggtctccagg ggattccgaa agaccgcaag ctcgagacga
     3481 ccttcccgaa aaccgccgca gcgaccgagc tgcccgagca gttcaaggtg gtcgcgttca
     3541 acatccacaa ggagccggcc gcaaagttca tcgcgggcat gcaggccgat cgcgccacgc
     3601 gcgacgccga cctcatcctc ctcgaggaag tgccgcgatt cgatggccaa gaaggctgca
     3661 gcgctgcatg cggcgccggc aagctactcg gctactacgc gatctacgcg cccgcgcacc
     3721 acgccgaaga gcgggacatc ggcgtcgcaa ttctgtctcg cgcgcccatc acatccgcgc
     3781 gcgtcatcga gctgccgtgg aacgacgtgc acttcaacgc cggccgtcgc gccgcccttg
     3841 ctgtgacgat ccagcaagcg ggccgcccga tcacggtcta tgccgtccac ctcgagaacc
     3901 gcctctcggt cggtgaccgc aagaagcaga tgacgccgat cctcgaggac gcaaagcgcc
     3961 aaccgacgcc cgtcctcatc gccggcgact tcaacacgaa tccattccac tgggtttcgc
     4021 actggctgcc gctccccacc gcgggtcatc aagcgacgaa gctcgagtcg ctcgtgcgtt
     4081 cgtacggctt cgatacgccg gtcaaggaca gcggcccgac tcaccgcttc atcggcatga
     4141 agctcgacgc gatctacacg cgcggcttcg agacgcgcaa cttcggtacc gcgcacgcaa
     4201 aggatgtctc tgaccacctc gcgctctggg cgacggtgaa cgcgaagcgc gcggccacga
     4261 agcctgttgc gcccgcagcg aacgtcgcga ccgtcgctgc aacgaagtag cgacctgcgt
     4321 cgtacgccgt cggagttttt cgctgacctt tcggcattga agcaaggcgg tgcgtcgcac
     4381 gtcggatcga catgcgtact ctcaccaccc tcggacttct cgcggttctc accggaacgg
     4441 ctgcggccga ccacggcgtg taccgcgacc gtagcttctc gtcacagcag tcgttgcgca
     4501 acgcgccgca aaaccgtcaa ccgcagacgg aagagcgtta tcaggagcgg aacaagctcg
     4561 ctgacgctgg gctgtcgccg tacagcaatc acgcggtcgt ccaactgccg caggccggcg
     4621 gatcactcga ctacctcgag ctgcgagcag gccgcacgcg cgtcgcgctg cgcgatgtcg
     4681 tcgtgcagtt tgccgatggc agcacgattc acacgggctc acgcggtgtt gtcgaaccgt
     4741 ttgaaggtcg cgtgatcgat ctaccgtcgg gctgcgcgca ggtcgtcgcg gtgatgccgg
     4801 aatatgaaac gccgggtcac ggcgcgagcc tcgaagtgtt cggcgttcac gagcatcgca
     4861 ctgggtgggg ctaccagccg aacagctacc agccgaacag ctaccagccg aacggctacc
     4921 agccgaccag ctaccagccg accggttacc agacgaacgg ctggggctac cgctgacgcg
     4981 caacgctcca ttccgctagc cgtctgtgcg ggcgcgctcg gatgacgttc gtcgtcgacg
     5041 agccaagctt cgagcatgac cgactcgcag ttggagctga ttagtcatcc gctctgcccc
     5101 ttcgtccagc gagctgccgc actcctcacc gaatgcggcg tcccgttcac gcaacgccac
     5161 gtcgacctcc agaacaagcc tgcgtggttt ctcgcgctct cgccgcgcgg caaagtaccg
     5221 gtgcttcgcg tcgacggcac gacgatcttc gagtcgaatg tcatcctcga gtacgtcgcc
     5281 gagcgcttcg cacctcagct cctcacgggt gacccgctgc tgcgcgctcg ccgccggatg
     5341 tggatgacga tcagcgatga cctgctgatg acgaactaca aggttgcagt tgccgccacg
     5401 cgcgcggacc gcgacagtgc ggtcgctgct gcaaaggaaa cgctcggccg cttcgagccg
     5461 ctgttgtcgg cgagaacgtt catcggcgtc gatccggcac aactgtcgtc gaacgacaac
     5521 gcgtcgaacg gggacaacgc agctcgcgcg tcgaacccgg acaacgcagc tcccgcgtcg
     5581 aaccgggaca acgcagctcc cgcgtcgaac cccgtcgacg cagcctcgat ggcaacgaag
     5641 cgcgccacag ccaaagacgc gcccggcctc atcgattttg ccgccgggcc aggcctcgtg
     5701 cggttcgaga agctcggccg cgagctcggc atcgacttct acgagggcct gcctcaggtc
     5761 gcgcgctggt cacgcgccat cgcgcaacgc gcggccttca cacgtacgct cgtcgctgac
     5821 ttcgacgcgc gctttcgcga attcgtccgt cacgagctcg ccgcctagct cgctagcgac
     5881 tgcgacttcc ggcaccgctc gcgcgattag ttcgccgagg cgcgggcgtc gacggctagc
     5941 tcgctagcga cggcgacttc cgcagcgctc gcgcgactag ttcgccgagg cgcgggcgtc
     6001 gacggccggc ttcgccgacg agaacggcgc ggaggccggc gactcggccg gcttggccga
     6061 cgacgtgctg gtgacgtcga ggttcttcgc attgaggtac gcgacttggc gcatcgccca
     6121 atcccacacg cgcgtcggga acaaccgcgc gaagaggatc acgaagttcg tcgagaacgg
     6181 cgcgacgtag cgagcagc
//
LOCUS       DATLBZ010000277        10186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6112845, whole genome shotgun sequence.
ACCESSION   DATLBZ010000277 DATLBZ010000000
VERSION     DATLBZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10186
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6112845"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VMZ53_14610"
     CDS             <1..634
                     /locus_tag="VMZ53_14610"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HUS29732.1"
                     /translation="GKSSLLNALVGKERALVAARPGTTRDYLEVGDDWNGVAVTIVDT
                     AGTRETSDEIEARGIALGEQRVTNADVVVVVNDHEHPWDDGARFGERALVVRSKADLG
                     GDDRGVLATAATTGLGLEELKRRVLMLAGVADREGSEDAFVTTARQQATAASARDGFT
                     AALAAWRNHKVFEVVALELRQGVHALAQLRGQEVGDRVLDEVFARFCIGK"
     gene            671..1852
                     /locus_tag="VMZ53_14615"
     CDS             671..1852
                     /locus_tag="VMZ53_14615"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HUS29733.1"
                     /translation="MSLDDDVRAELRELEAVHRLRTPRVVDGMQGPRVVLDGVEVTSF
                     ASNDYLSLAGDRRLARAAASAVDEDGTGAGASRLIVGNHRRHMALEASIADWLCVDGA
                     RLFNSGFAANVGVLTSLLGPDDVVFSDELNHASIIDGCRLSRARVVVYPHCDVAALER
                     ALSSDGVASARRRIVVSETLFSMDGDIADVAALAALARRHEAALILDEAHAIGARGPE
                     GRGVAAEANVSPDVLVGTCGKALGSFGAFAATSRAVADLLWNRARPLVFSTGLPPMIC
                     AAVQAALEIVRGAEGQERRQDLAAVARELRRLVPTLGGYPDSAIAPLLIGDDRVAVQL
                     TEHLLERRLYVQGIRPPTVPEGTARLRISLAGGHTNQDVVHLASALRDAPRQFPVKRS
                     P"
     gene            1951..3243
                     /gene="bioA"
                     /locus_tag="VMZ53_14620"
     CDS             1951..3243
                     /gene="bioA"
                     /locus_tag="VMZ53_14620"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HUS29734.1"
                     /translation="MWHPFTQHREWAPLVIARGDGNFLIDTEGRRYLDGVSSLWCNVH
                     GHHHPRLDAALRQQLDQLAHSTFLGLSHEPGIRLAAELLDVAPRGTTGGSAERGPLAR
                     VFYSDSGSTAVEIALKQSFQYWALRGRPKKQRFLRMTDAYHGDTLGAVGVGGIDVFHR
                     IFGPLLVQSIPIPTPAGTDGSASLARIESELQARSFEIAAFIFEPLIQGAAGMLVHPP
                     GFLAKVAELCQRHNVHLILDEVATGFGRTGRMFACEHELVSPDFLCLAKGISGGYLPL
                     AATLCTEEIFSAFLGTRAEMKHFFHGHTYTANPLACAVGLESLRLLREQTLANAQARE
                     PEIAAALSRVGQLPGVKEARRVGLMIGIELEGRADRFVGVEVCERARAHGVILRPLGD
                     VVVWMPPLSIRSEEVDLLESATTAAIRESCGSSFHVKQ"
     gene            3240..3905
                     /gene="bioD"
                     /locus_tag="VMZ53_14625"
     CDS             3240..3905
                     /gene="bioD"
                     /locus_tag="VMZ53_14625"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HUS29735.1"
                     /translation="MKGYFVTGTDTGVGKTFVGAALAKRARELGERVFGFKPIETGVP
                     AQREGEDQVLLCDAAGGWQTGELRGVYRYRQPAAPRVAAEAERTKIDLDRIARVFREG
                     LAATHPNGGTPTFAVTEGAGGWRVPITDTADTSTLAKAIGLPVLVVARAGLGTINHSL
                     LTLEAIERDGCEIAALVLNVRHDDDRIFARSNEQEIRTRWPGRIVYFLEPTDLDPLIV
                     GPS"
     gene            complement(3902..4534)
                     /locus_tag="VMZ53_14630"
     CDS             complement(3902..4534)
                     /locus_tag="VMZ53_14630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HUS29736.1"
                     /translation="MTSKLHAAFLDLFEKWNKSINLSAASTRAELQEHLADSLHVIPH
                     LRAHLLDRNDARQNASQLTPVVNPETAALGAETETPRVLDVGSGGGFPVVIAAIEMPE
                     VHFTALEPVHKKHAFLRTAARELKLSNLEALARRLDDHDVADYDVAMSRATFDLAEWL
                     AIGSKRVRPGGLVLGFEAVPRTDLPPATVRHPYALAGKQRAIVALSVGGS"
     gene            complement(4531..5736)
                     /locus_tag="VMZ53_14635"
     CDS             complement(4531..5736)
                     /locus_tag="VMZ53_14635"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HUS29737.1"
                     /translation="MRVELPYGRFPLAFDAGAHPVTVVKAAELPEPPPVRQLLISALA
                     SAARPTGSLAEASERVTIIVSDPTRKEPRQELLTALSEYIDTKTRVTLAVATGTHGPC
                     RIEDLGISQRSLQNAHAIVNHDGHSDVDLVDLGTTTRGTPIRVHRCLVEADVVIATGC
                     IRPHYFAGFGAGAKAFFPGLGQAAAIRMNHRLKTEPLSRAGIVDGNPCREDIEEAVAK
                     IPTRKFILNGVAGPDDRIHAVVAGGRDDSARFTDRHETIDEVFRRGVSISRPWFTARA
                     RRAPLVIASDDLPVTASLYQAAKIAAAVAPLVEENGTLALVADCHEGVGPLETVNEAI
                     FRIGILPRLPQGVRLVLISSLAPDVVAQTLCNYAPSIDALIAELALPSSAPITVVPRA
                     SQLLCEAHS"
     gene            5921..8959
                     /locus_tag="VMZ53_14640"
     CDS             5921..8959
                     /locus_tag="VMZ53_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUS29738.1"
                     /translation="MDGDTRSLRVLEVSLRKAGFSVTPAVSVGDALDKLEVNTPDLII
                     SETKFSTELDGFELRRRLLATQAWSGIPFIFLTAETAIENKIKGLELGVDDYLTKPIY
                     IKEIITRINILLQKRQRQFLDAKKDGTRFAGRVADMPVVDVIQTIEVSRKSGVIQFVG
                     GPNRQAAIYFRDGKVIDAEAGTLQGEDAVYRLLTWNDGEFEVEFRTVRRREAITTSSQ
                     GLLMEGMRRLDEWSRLLEQLPSLSHRFEIDAAELSMRLGEVPDENNRILRLFDGKRTL
                     IEVIDASDFGDLECLQAISRLYFEGMLVDLDQGTEERPRRDTGRPMPLAMLEDGGSSR
                     RTPMEVVASGPIDLTNIEARAYAQEIPVPLPMGQSGPVIAAIEAQPSMPVRTNITQIE
                     SGPVEASGPTQLAAAAAVPEMPRARRESGEIVSDQEVLSEEEAPIPGPLLGGYRASSL
                     RLIDEAVAAAQLIEPSLFTDEELVAAGVKRPSPDAKPSEDKPIARVQLEKAPSNGGVP
                     RTRRPATLPPPLPMSMSARLKKEIDDAPMLEADLVSGEIKVPTEAELADAPMLEADLD
                     TSGEHTPVAPPPRPSAPSAPPPIPPSKSEPDTSASNGAAATASSASPAPVSPASEEDS
                     GGVPTAIATEEESNELADEGPTDPSRRRFAATVKRDDDAALRMIGSHGKDRAEASGEL
                     KPVSPKGAESETQREVVTIMPRRNTREIPQIPDGKPAEASTAAEAKTDAKTDEAKTEK
                     KDAKPAVEPPRTPPRRVSVAGGVSKASGPGKLAGILLTLASVLIGATVFSYCRKNKPA
                     TAVATREDAAAVAPEIDAEYYAGFGIDAPAVVTTADATQVVQQRPDAAVQLRPDATQV
                     AMQTPDAAVPRPDAAQVVQVRPDAAQVAVSTADAGIDKTKEAKALYDKAHAALEDGDP
                     QAALDMLDKSLALRKTARTYLEKARALQRMQKIDDALGAVDSAIKMSGEYAPAWEQKG
                     MMLWSAQRYSEARPVLEHYLELDPTGKKADTIRSMLEENK"
     gene            9052..10068
                     /gene="tsaD"
                     /locus_tag="VMZ53_14645"
     CDS             9052..10068
                     /gene="tsaD"
                     /locus_tag="VMZ53_14645"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HUS29739.1"
                     /translation="MRVLGLESSCDETAAAIVEDGRIVLSDVVASQNEVHARYGGVVP
                     ELASRAHILNVVPVVQAALDRASMKLDDVDGIAVTNAPGLVGALLVGLQTAKALAWVT
                     GKPLVGVHHLEGHLSAIYLEPDPPPFPHLALIVSGGHTSLVRVDGHGQLTELGATRDD
                     AAGEAFDKGAKLLGLGYPGGAVIDKLAQTGDPKAVPFPRSMTQGEHGAEFSFSGLKTA
                     LLHHVRAQGVPDGQALADLCASYQAAIVEVLVRKTRKQARILGMQHVQICGGVAANSG
                     LRKAMLAEGEEYDFRVYVPPIARCTDNAAMIAAAGYYHLLAGERDGLELDANPSKPLA
                     RAPA"
BASE COUNT         1772 a         3317 c         3413 g         1684 t
ORIGIN      
        1 cggaaagtca tcgctgctga atgcgctcgt cggtaaggag cgtgcgctcg tcgccgctcg
       61 gcccggaacg acgcgcgact acctcgaggt cggcgacgat tggaacggcg tcgcggtcac
      121 gatcgtcgat accgccggaa cgcgcgagac gtccgacgaa atcgaggcac gtggcatcgc
      181 gctcggcgag cagcgcgtga caaacgcaga cgtggttgtc gtcgtcaacg atcacgagca
      241 tccgtgggat gacggcgcgc ggttcggcga gcgagctctc gtcgtccgca gcaaggcgga
      301 tctcggtggc gacgatcggg gcgtgctcgc gaccgcggcc acgacgggcc tgggcctcga
      361 agagctgaag cggcgcgtgc tgatgctcgc aggtgtcgcg gatcgcgaag gcagcgagga
      421 cgcattcgtg acgaccgcgc gccagcaggc gacggccgcc tcggctcgcg atggattcac
      481 ggcggcgctc gccgcatggc gcaaccataa ggttttcgaa gtcgtggcgc tcgagcttcg
      541 ccaaggggtg cacgcactcg cgcagttgcg aggccaggaa gtcggcgacc gcgtgctcga
      601 cgaggtgttt gcgcggttct gtatcggaaa gtgactacaa ctaggctgcg cgccgctata
      661 gtcgcgcgcc gtgagcctcg acgacgacgt gcgcgccgag cttcgtgagc tcgaggcggt
      721 acatcgcctg cgcacgccac gcgtcgtcga cggcatgcag ggcccgcgcg tcgtgctcga
      781 cggtgtcgaa gtcacgagct ttgcctcgaa tgattatctc tcgctcgcgg gagatcgtcg
      841 gctcgctcgc gccgccgctt cggccgtcga cgaggatggt accggcgccg gcgcatcgcg
      901 attgatcgtc ggcaatcacc gtcgccacat ggcgctcgag gcgtcgattg ccgattggct
      961 gtgcgtcgac ggagctcggc tcttcaacag cggatttgca gcaaacgtcg gcgtgctgac
     1021 gtcgttgctc ggccctgacg acgtcgtgtt ctccgacgag ctcaatcacg ccagcatcat
     1081 cgatggctgc cggctctctc gcgcgcgcgt cgttgtctat ccgcactgcg atgttgctgc
     1141 gctcgaacgc gcgttgtcgt cggatggcgt cgcctccgcc cgcaggcgca tcgtcgtcag
     1201 cgagacgctg ttctcgatgg acggcgacat cgcggatgtc gcggcgctcg ccgccctcgc
     1261 ccgccgtcac gaagctgcgc tgatcctcga cgaggcgcat gcgatcggtg cacgtggtcc
     1321 cgaaggtcga ggtgtcgccg ccgaggcgaa cgtgtcgccg gacgtcctcg tcgggacgtg
     1381 cggcaaggca ctcggcagct tcggggcatt cgccgcaacg tcgcgcgccg tcgccgatct
     1441 gctgtggaat cgcgcgcggc cgctcgtgtt ctcgacgggc ttaccgccga tgatctgcgc
     1501 ggcggtgcag gccgcgctcg aaatcgttcg cggagccgaa ggtcaggagc gccgtcagga
     1561 tctggcggcg gtcgctcgcg agctccgccg gctcgttccg acgctgggtg gttatcccga
     1621 ctcggcgatc gcgccgctct tgatcggcga cgaccgggtc gcggtccagc tgaccgagca
     1681 cctgctcgag cgtcgcctct acgtgcaggg catccggccg ccgacggttc cagaagggac
     1741 tgcgcgtctt cggattagcc tcgcaggtgg tcataccaat caggacgttg tccatctcgc
     1801 ctcggctctc cgtgacgcgc cgcgtcagtt tcccgtgaaa cgttcgccat gaaaccagga
     1861 cacccgacga gcgccgcgac ttcggctggg gatagtgccg cgacttcggc tggcgatagt
     1921 gccgagcttt ctgctctcga tcagaaagtc ctgtggcatc cgttcactca gcacagggag
     1981 tgggccccgc tcgtcattgc acgtggcgac gggaacttcc tgatcgacac cgagggccgc
     2041 aggtacctcg atggagtcag ctcgctttgg tgcaacgtcc acggccacca tcacccgcga
     2101 ctcgacgcgg ccttgcgtca gcagctcgac caactcgccc actcgacgtt tcttgggctg
     2161 tcgcacgaac ccgggattcg cctcgcggcc gagctgctcg acgttgctcc gcgcggcacg
     2221 accggtggct ccgcagagcg cggtccactg gctcgcgtct tctactcgga ttctggctcc
     2281 acggcggtcg agattgcact caagcagtcg ttccaatact gggcactgcg cgggcggcca
     2341 aagaagcagc gcttccttcg aatgacggac gcgtatcacg gcgacacgct cggcgcagta
     2401 ggcgtcggcg ggatcgacgt cttccaccga atcttcggtc cactactcgt ccagtccatt
     2461 ccgatcccga cgccagcggg caccgacggc tccgcgtccc tggctcgcat cgagtccgag
     2521 ctgcaagcgc gctctttcga gattgctgcc ttcatcttcg agccgctcat ccagggcgcc
     2581 gccgggatgc tcgtgcatcc gccgggattc ctggcgaagg tcgccgagct ctgccagcgc
     2641 cacaacgtcc acctgatcct cgacgaggtc gcgactggct tcggccgaac cggccggatg
     2701 ttcgcgtgcg agcacgagct cgtctcgccg gacttccttt gcctcgcgaa ggggatctcc
     2761 ggcggctacc tcccgctggc cgcgaccctg tgcaccgagg agatcttcag tgcctttcta
     2821 ggtacgcggg ctgagatgaa gcacttcttc cacgggcaca cgtacacggc aaacccgctc
     2881 gcttgcgcgg taggtctcga gtcgctccgc ctactccgcg agcagaccct cgcgaacgcg
     2941 caggcccgtg agcccgagat cgctgctgcg ctgtcgcgag tcggtcagct ccctggcgtc
     3001 aaagaagctc gtcgcgtcgg ccttatgatc ggaatcgagc tcgagggtcg agccgatcgc
     3061 tttgtgggcg tcgaggtctg cgagcgagct cgggcccatg gcgtgatcct tcgtccgctt
     3121 ggggacgtgg tcgtttggat gccgcccctc agcatccggt ccgaagaggt cgacctactc
     3181 gagtcggcaa cgaccgcggc gattcgcgag agctgcgggt cttcgtttca cgtgaaacag
     3241 tgaagggcta cttcgtcacg ggcacagaca cgggcgtcgg caagacgttc gtcggcgccg
     3301 ctctcgccaa gcgcgcccgg gagctcggcg agagagtctt cggcttcaag ccaatcgaga
     3361 ctggagttcc tgctcagcgc gaaggcgaag accaagtgct gctctgcgat gccgcgggcg
     3421 gatggcagac cggcgagctc cgcggcgtgt atcgctaccg ccaaccagca gcgccgcgag
     3481 tggcagcgga ggctgaacgc acgaagatcg atctcgatcg gatcgcgcgg gtcttccgag
     3541 aaggcctggc agcaacgcat ccgaacggag ggacgccgac cttcgcggta acggagggcg
     3601 ctggtggctg gcgcgtcccg atcacggata ccgcagacac ctcgacgctc gcgaaggcga
     3661 tcggtctgcc ggtcctcgtc gtcgcgcgcg ctgggctcgg gacgatcaac cacagcctac
     3721 tcaccctcga agcgatcgaa cgagacggct gcgagatcgc agcgctcgtc ctcaatgttc
     3781 ggcacgacga cgaccgcatc tttgcgcgaa gcaacgagca ggagatccgc acgcgctggc
     3841 ccggccgaat cgtttacttc ctcgagccca cggacctgga cccgctcatc gtcggtccga
     3901 gctagctacc accaaccgac aacgcaacga tcgctcgctg cttgccggca agcgcgtacg
     3961 gatgccgaac agttgccggc ggaagatccg tacgcggcac cgcctcgaaa ccaagcacca
     4021 gcccaccagg ccgcacgcgc ttgctcccga tcgcgagcca ctccgcaagg tcaaacgtcg
     4081 cgcgcgacat cgcgacgtca tagtcggcga catcgtgatc gtcgagtcgt cgcgcgagcg
     4141 cctcgaggtt ggagagcttg agctcgcgag cagccgtccg cagaaacgca tgcttcttgt
     4201 gcaccggctc gagtgccgtg aagtggacct cgggcatttc gatcgcggca atgacgaccg
     4261 gaaagccgcc gcccgatcca acgtcgagca cgcgcggcgt ttccgtctcc gcgcccagcg
     4321 ccgcagtctc cggattcact accggggtaa gttgtgacgc gttttgacgc gcgtcatttc
     4381 ggtcgaggag atgagcacgc aggtgcggaa tgacgtgcag gctgtcggcg aggtgctctt
     4441 gcagctcggc acgagtagag gcggccgaga gattgatgct cttgttccat ttctcgaaga
     4501 ggtcgagaaa ggcagcgtgg agcttgctcg tcatgaatgt gcctcacaca gcagctggct
     4561 cgctcgtgga acgaccgtga tcggagctga cgagggcagc gcgagctcgg cgatgagcgc
     4621 gtcgatcgag ggcgcgtagt tgcaaagcgt ttgcgcgacg acgtcaggcg cgagcgacga
     4681 gatcaggacg agccgaacgc cttggggcag gcgcggcagg atgccgatac gaaagatcgc
     4741 ttcgttgacc gtctcgagcg ggcccacgcc ttcatggcaa tcggccacca gcgcgagcgt
     4801 gccgttctct tcaacgagcg gggcgacggc ggctgcgatc tttgctgctt ggtaaagcga
     4861 ggcggtgacc ggaagatcgt ccgaagcgat cacgaggggc gcgcggcgcg cacgagcggt
     4921 gaaccacggc cgcgagatgc tcacgccacg gcgaaacact tcgtcgatcg tctcgtggcg
     4981 gtccgtgaaa cgagcgctgt cgtcgcgacc gccggcgacc accgcgtgaa tccgatcgtc
     5041 gggtccggcg accccgttga ggatgaactt tcgcgtcggg attttcgcga ccgcctcttc
     5101 gatgtcttcg cgacatggat ttccgtcgac gattcctgcg cgcgagagcg gctcggtctt
     5161 gaggcgatgg ttcatgcgaa tcgccgcggc ttggccgagc ccggggaaga acgcctttgc
     5221 gccggcaccg aagccagcga agtagtgcgg gcggatgcag cccgttgcga taacgacgtc
     5281 ggcttctacg aggcaacggt gcacgcgaat tggcgtgccg cgcgtcgtcg tgccgaggtc
     5341 gacgagatcg acgtcgctgt ggccatcgtg attgacgatc gcgtgcgcgt tctgcagaga
     5401 gcgctgtgag atgccgaggt cttcgatgcg gcaagggccg tgcgtgccag tcgcgactgc
     5461 gagcgtgacg cgcgtcttcg tatcgatgta ctcgctgagc gccgtcagga gctcctgcct
     5521 tggttccttc cgcgtcgggt cgctgacgat aatcgtgacg cgctcgctgg cctccgcgag
     5581 tgagccggtt ggccgggcgg cacttgcgag cgcgctgatg agcaactgac gcaccggcgg
     5641 cggctcggga agctccgcgg ccttgaccac cgtcaccggg tgcgcgccag cgtcgaacgc
     5701 gagcggaaat cggccatatg gaagctcgac tctcaccggc agctacccga gcgtcccctc
     5761 ggcgattagt tgtttaagtg gggtccgtct gccgatcagc aggtttcgga cgggggagtg
     5821 cacgacgaga gggtacgttg ctggcggcgt ctcgtgtagt tggttacgat tcttcggccg
     5881 gcctccgtcg gtgtccgtgt cgaagcaaag cttgctactg gtggacgggg acactcgcag
     5941 tctgcgagtg ctcgaggtgt ctttacgcaa ggcagggttc agcgtgaccc cggccgtcag
     6001 cgttggcgac gcgctcgaca agctcgaggt caacacgccc gacctcatca tctccgagac
     6061 caagttctcg accgagctcg atggcttcga gctgcggcgg cgattgctcg cgacccaagc
     6121 gtggtcgggt atcccgttca tcttcctgac cgccgagacc gcgatcgaaa acaagatcaa
     6181 aggcctcgag ctcggcgtcg acgactacct gacgaagccg atctacatca aagagatcat
     6241 cactcgcatc aacatcctgc tgcagaagcg gcagcgtcag ttcctcgatg cgaagaagga
     6301 cggcacacgg ttcgcgggac gtgtcgcgga catgccggtc gtcgatgtca ttcagacgat
     6361 cgaagtcagc cggaagtcgg gcgtcatcca gtttgtcggc gggccgaatc ggcaagccgc
     6421 gatctacttc cgcgatggca aggtgatcga cgccgaggcg ggcacgctcc agggcgaaga
     6481 cgccgtctat cgcctgctca cgtggaacga tggcgagttc gaggtcgagt tccgcacggt
     6541 gcgccggcgt gaagcgatca cgacgagctc gcaaggcctg ctcatggagg gcatgcgccg
     6601 cctcgacgag tggtcgcgcc tgctcgagca gctgccgtcg ctgtcgcatc gattcgagat
     6661 cgacgcagcc gagctctcga tgcgtcttgg cgaagttccc gacgagaaca atcgcatcct
     6721 gcgtctgttc gatggcaagc gcacgctgat cgaagtgatc gatgcgtctg acttcggcga
     6781 cctcgagtgc ctgcaggcga tctcccgcct ctacttcgaa ggcatgctcg tcgatctcga
     6841 ccagggcacg gaagagcgtc cgcgtcgcga tacgggaagg ccgatgccgc tcgcgatgct
     6901 cgaagacggc ggctcgagcc ggcgcactcc gatggaggtc gtcgcatcgg gaccgatcga
     6961 tctaacgaac atcgaggcgc gcgcctacgc ccaagagatt ccggtgccgc tgccgatggg
     7021 ccaatcgggc cccgtgatcg cagcgatcga agcgcagcct tcgatgccgg tgcggacgaa
     7081 catcacgcag atcgagagtg gccccgtcga agcgtcggga cctacgcagc tcgcagcggc
     7141 cgcggccgtc cccgagatgc cgcgcgcacg acgcgagtcc ggcgaaatcg tgtccgacca
     7201 ggaagtgctc tccgaagaag aggcgccgat tcctggcccg ttgctcggcg gctatcgcgc
     7261 atcgagcttg cgtctgatcg acgaggccgt tgccgccgcg cagctgatcg agccgtcgct
     7321 gttcaccgac gaggagctcg tggccgcggg cgtaaagcgt ccgtcgccgg atgccaaacc
     7381 gagcgaggac aaaccgatcg cgcgcgtcca gctcgagaag gcgccgtcga acggcggtgt
     7441 gcctcgcacg cgccggccgg caacgctgcc accaccgctg ccgatgtcga tgtcggcacg
     7501 tctcaagaaa gagatcgacg acgcgccgat gctcgaagcc gatctcgtca gcggcgagat
     7561 caaagttccg accgaagccg agctcgcgga cgcgccgatg ctcgaagcgg atctcgacac
     7621 gagcggagag cacacgcctg tcgcgccgcc gccacgcccg tcagcgccgt ccgctccgcc
     7681 tccgattccg ccctcgaagt ccgagcccga cacatcggcg agcaatggcg ccgctgcaac
     7741 cgcgtcgtcg gcatcgcctg cgcctgtttc gcctgcatcg gaagaagatt cgggcggcgt
     7801 tccgacggcg atcgcgaccg aagaagagag caacgagctc gccgacgaag gacctacgga
     7861 tccatcgcgt cgtcgattcg cagcgacggt caagcgcgat gatgatgccg cgctgcggat
     7921 gatcggctcg cacggcaagg atcgcgcaga ggcatccggt gagctcaagc ctgtttcgcc
     7981 gaagggcgcg gagagcgaga cgcagcgcga ggtcgtcacg atcatgcctc gccgcaacac
     8041 gcgcgagatc ccgcagatcc cagacggcaa gccggcggaa gcgagcaccg ctgccgaagc
     8101 caagaccgac gcgaagaccg acgaagcgaa gaccgagaag aaagatgcga agccggccgt
     8161 tgaaccaccg cgcacgcctc ctcgtcgcgt gtcggttgcc ggcggggtat cgaaagcatc
     8221 gggcccgggc aagctcgccg gcatcctgct cacgctcgca tccgtcctca tcggtgcgac
     8281 ggtcttcagc tactgccgca agaacaagcc ggccaccgcg gttgcgacgc gagaagatgc
     8341 agccgcggta gcgcccgaga tcgacgccga gtactacgca ggcttcggga tcgacgcgcc
     8401 ggcggtcgtc acgacggcgg atgcgacgca agtcgtgcag caacgtcccg acgcggccgt
     8461 gcaactgcgg cccgatgcga cgcaagtcgc gatgcaaacg cccgatgctg cggtgccgcg
     8521 gcccgatgca gcccaggtcg tgcaggtccg tcccgacgca gcgcaggtcg cggtgtcgac
     8581 cgcggatgca ggcatcgaca agacgaagga agcgaaggcg ctctacgaca aggcgcacgc
     8641 cgcactcgag gacggcgatc cgcaggccgc gctcgatatg ctcgacaagt cgctcgcgct
     8701 ccgcaagacg gcgcgcacgt acctcgagaa agcgcgcgca ctgcagcgca tgcagaagat
     8761 cgacgacgcg ctcggtgcag tcgactccgc gatcaagatg agcggcgagt acgcgccggc
     8821 atgggagcag aagggcatga tgctgtggtc cgcgcagcgc tactcggaag cgcgccccgt
     8881 gctcgagcac tacttggagc tcgatccgac cggcaagaag gccgacacga tccgctcgat
     8941 gctcgaagag aacaaatgag gccgagggat ctcgcgtgtt ccgagtcctc gaggatcacg
     9001 cggaccttgg gccgaatgcg tcccgtggac aaatagggta agaacccgcc gatgcgtgtc
     9061 ctcggcctcg aaagctcgtg cgacgagacc gccgcggcga tcgtcgagga tggccgcatc
     9121 gtgttgtccg acgtcgtcgc gtcgcagaac gaagtgcacg cgcgatatgg cggcgtcgtg
     9181 cccgagctcg catcgcgtgc gcacattctc aacgtcgtgc ccgtcgtcca ggcggcactc
     9241 gatcgcgcga gcatgaagct cgacgacgtc gacgggatcg ccgtcacgaa tgcgcccggc
     9301 ctcgtcggcg cgttgctcgt tggactgcag accgcgaagg cgctcgcgtg ggtcaccggc
     9361 aagccgctcg tcggcgtcca ccatctcgag ggccatctga gcgcgatcta tctcgagcct
     9421 gacccaccgc cgtttccgca tctcgcgctg atcgtatcgg gaggtcatac gtcgctcgtg
     9481 cgtgtcgatg gacacggtca gctcaccgag ctcggcgcga cgcgcgatga tgcagcgggc
     9541 gaagcgttcg acaaaggagc caagctgctc gggctcggct atccgggcgg cgccgtgatc
     9601 gacaagctcg cgcagactgg cgatccgaaa gcagttccgt ttccgcgctc gatgacgcaa
     9661 ggcgagcacg gcgcagagtt ttcgttctcg gggctcaaga ccgcgctgct gcatcacgtg
     9721 cgcgcgcaag gcgttcccga cgggcaggcg ctggccgacc tgtgcgcgag ctatcaagct
     9781 gcgatcgtcg aggtcctcgt gcgcaagacg cggaagcagg cgcgcattct cggcatgcag
     9841 cacgtgcaga tctgcggcgg cgtggcggcg aattccgggc tgcgcaaggc gatgctcgcc
     9901 gagggtgagg aatacgactt tcgcgtttac gtgccgccga tcgcgcgctg caccgacaac
     9961 gccgcgatga tcgccgctgc gggctactac catttgctcg ccggcgagcg cgatggcctc
    10021 gagctcgacg cgaatccgag caagccgctc gcacgggctc cggcatgagt gggggcaacg
    10081 gcacgaacgg caatggcaac ggcaacggca atggccgcga gtcgcacacg aggctcgccg
    10141 aagcgatcgg gccatttccc gacgcgcgcg tgctgctcga gcgcta
//
LOCUS       DATLBZ010000278        24309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6113601, whole genome shotgun sequence.
ACCESSION   DATLBZ010000278 DATLBZ010000000
VERSION     DATLBZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 24309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24309
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6113601"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VMZ53_14650"
     CDS             <1..610
                     /locus_tag="VMZ53_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29740.1"
                     /translation="SIFVTIGFAFMPGHVAEAFSDKKQPAKQAHDAFAQFQAKRATTI
                     KAGHDAAKGSAAFLKLDAEERATLRDAPAAKPKLKPFNPPKLVPNLQYRCDQAHMLIP
                     SVHTPGPIASFESERDMREQCRPFAVDPTDADKVCTNPELLHCGIGDENNREFCCPKT
                     HPIFNVCDKQCYRNTDFNNALEDKDEGFHCGTSRDCGATIKP"
     gene            607..1590
                     /locus_tag="VMZ53_14655"
     CDS             607..1590
                     /locus_tag="VMZ53_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29741.1"
                     /translation="MTRVALAAIVALAACQPWYRDAERAGQRATDTKYSSKLIADANA
                     ARDRGDHAGAASLLRRAITEIPDQPADTFLSLARESFAANDIAGGRAACRRGMELWPN
                     DTSFRALLIDQKVRDDLTALAIEEAGHKTFSDLAADSSFTTVVDALKRAMDAEHGDTA
                     AGELALWLAHYGVPDHVLLVGAREAAADKVSRLMETTPALEGLRTAADRADAELAAGR
                     FARALALYSDVYRLLPASAISAHFANFAKAAQQATDPEAIDSGAYALAVQGDADAQAG
                     NLGLAIHSYRKAVARAPWWADARRNLAALYDSAGMTEMANAERAFADRLGR"
     gene            complement(1587..4892)
                     /locus_tag="VMZ53_14660"
     CDS             complement(1587..4892)
                     /locus_tag="VMZ53_14660"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS29742.1"
                     /translation="MHRIVLLACALLPALAHADRVTKPPVVELSERVKPVAQDKAPPP
                     KPVATPDSIMDSQLAAVPYREEQVDLLKKLIKSTPDSAADEKADYYFRLGELYASQQR
                     VYRLQSARAEIAGDKAAAKAAADKAHSFMIGAVSTYKDLTDNDSFRNFPKMDLALFYY
                     AYTLQSGKYVKEARSVFDKLLKNYPSSKYVPEAHLAFADYYFESGQLADAEARYKRVL
                     EFPSSNAYWYAMYKLGWIDLNLAKHQDALEVFYKVANATKANAKYEVLNRAAKKDFVR
                     AYAEIGKADKAFDAFKRVDTKAAFGMLETLADFYLGQGKSDRAIYVLRELMKLQPTSP
                     RVCTWQYEVAHATLALSGATNADKVKEIVDLDRVYLAVAGKLPKGDADECRDNASAMA
                     GELARAYHSEWTKTKSVDSFSASEKLYKAYLATFPKASDFPATHYYFAELLWSRAETE
                     KDARLQTQLWENAGVEFTDVVKAGGLDAVLSKEAAYAAVLAFLYAHDRDPRSAAPAKA
                     GEIPERDQKMLAAFDLYFATVKDAKNDDELVRIKFTKGNVLRRYARFDQAIPIFRDIV
                     AKYPTHDLAEDAANLWLDSLNQKHDEVGLVAAARELLANGKFLDDKPKAKEVAEDIEL
                     TALRKIAEKLEKEARATKDPAKLVECGNAYLAIYNRNPEAKKNDEVLYNAGVCYEDGH
                     SIGVAIRMYGLLEQYYPQSPVLPRAIARLGKAYGDIAFYDRAADRLEQYAKKFAAEED
                     AHDAMNDAVLYRKGTGDDAKAIEDTKFFVRTFGKSNPAEVANAAFALTSVYEKKGDHE
                     AVIKHLREYIRVHGDKGGADKVVIAHARIGELLFEDSCPVKTVDDACVKITRERAVAT
                     RGRLARLPTQCGPDTKIKLTLVPRDERKVRDALAELATAAKHATKATTGDTAGVRYHL
                     ALARMIEANVEYEKYLGLAFPQNLDFDPKNKAVRDKSLARFDAWFAAKAKAGEAVSKK
                     YVSVLELNDSANSIAARARLAQIAQNFSDALFTAEIPKDVRSGSYAEDKIEAYCDVLT
                     TKADPLAVAAVKNYDVCLAKSTELGWFSEWSRLCERELGQLEPEQYPTATERHAESDR
                     FAAVIALETPPTL"
     gene            complement(4970..6763)
                     /locus_tag="VMZ53_14665"
     CDS             complement(4970..6763)
                     /locus_tag="VMZ53_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29743.1"
                     /translation="MAACSHGAGLKTQVAACTSKAGGDPGACLAVANDRMMAKDEAGA
                     KQYVEAATDAIGPTCLHDHAAAGCFQTIVLLLGEEPVGLLASFKVSEDLRVLAPKGEG
                     DDATNPRTRARTALLGMCGNPAGDAIERQRACIVLGDLVAEEQAKRCGEGCDVATAKL
                     GGWGQSEIIDAYGAACKVASERTDAARASAFADAVRKLYAVTGSGEPACGLATSPLHG
                     ASIADALDNIRRIQQDANVRTRGAKTSQQREAIRIAQMKQNAVAEQAKAQSDADAKAA
                     HDLKVALDSANWGIAYELIRKRDARSPVDEASATALVHVWDSFLAWAIQQTTPMAVYV
                     DVADAFSKLPASHALPALVTGLRDRALSDLKGRSKATRGRGGAWLYAALAARVAGSSS
                     EEARVAFEQYEKLVAAVKTSVAFDKLSPACTPLVKETAGKRVRATAALACSVIPEKKW
                     TAQEPMTIKQHVVKDGGEEDVEQVVQVEVAHRSFSVTVHGTVTVQGKTVPVDFEETLD
                     DKDGTSTRTFEQARKAAMAAVVDAVVAPLDAATAAKELTAAQNALKVQRQGAAEDHLV
                     NHALLVGSSAELDEILSPFNVTFGELVQASR"
     gene            complement(6839..7501)
                     /locus_tag="VMZ53_14670"
     CDS             complement(6839..7501)
                     /locus_tag="VMZ53_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29744.1"
                     /translation="MKRWGPLVGALVGGAIGFAIGWHFASNDVNKLGDWRNGAARFIG
                     MTSAIGMIVGYVVAMLATRGTHVTRDGFTVSYRPAEAVPQGYRELKTLTVGDLVERLQ
                     AVGYRPVIEACDEVGERGKPGDASIPLVGANIALVDPGVRGWVRVELPIPAAGQARAL
                     GVVEIWSTTGESAEELALFTLRSLGELVHGVAASRESSRLSEDSVNMLTAGLAERPQH
                     RK"
     gene            7571..8329
                     /locus_tag="VMZ53_14675"
     CDS             7571..8329
                     /locus_tag="VMZ53_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS29745.1"
                     /translation="MRQRTRLFKVSRDSQREGSATGCKSVACKLLGSDAVSGGVLKSR
                     AMLVDDHPLFRTGLAITLAKEPDLHVTADVGSAVEALEAMRASAIDIAVIDVLMPTTT
                     GVSLAAELLELRPSCKILALSVLDEPMIIATMLRAGALGYALKTQPPNEITQAIRIVL
                     SGARYLPPFVSHEAVLGLVADTVERPMDRLTRREREVFDLLIRGHSNDEIATRLFISR
                     RTVETHRQRITKKLATHSILDMIRLAARQGALCD"
     gene            complement(8343..9194)
                     /locus_tag="VMZ53_14680"
     CDS             complement(8343..9194)
                     /locus_tag="VMZ53_14680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS29746.1"
                     /translation="MVQYYGNICQAITELAVEVGPQITNADFQTLNLCLDIAIADAVT
                     EFARQSDESIIGRNVEQLGFLAHELRNLLNAATLAFEAVRSGSVGVDGTTANLVVRSH
                     AAMDDLVARALAEVRLEAGPPRDERVSLAEIFEHVEIAAATHAKSQDVMLSIKLVPPG
                     LAVDGDTQIVNSIVINLVQNACKFTQRHSHVTVGVRVLDDQVFIDVADQCGGLPRGKL
                     EELFMPYAQRHSNRAGVGLGLAISRRGARAIGGDIMVTDMPGIGCVFTVALRRSAEEQ
                     RPEPPAA"
     gene            9697..10713
                     /locus_tag="VMZ53_14685"
     CDS             9697..10713
                     /locus_tag="VMZ53_14685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS29747.1"
                     /translation="MTDAELEHGIPLFLDQLIGMLRKELGPPIEASNQAAVLTATAEK
                     HGNEIFRKGFALDQVVHDYGDLCQSVTELAVETDVPITVDEFRTFNRCLDNAIADAVT
                     AFGDVRDKTISAEGAEAMNERLGSLAHELRNSLNAAILAFDAIKTGSVPVTGATGAVL
                     DRSLLALRDVIDRSLAEVRLGAGLDAHRESINVREFVQELQVAAAMDAKTREITFNVS
                     PIDNRLAVDTDRQMLGSAISNLLQNAFKFSRHNGSVLLTARAVDDRVILEVEDECGGL
                     PAGKAEELFRPFEQKSGDRTGLGLGLSISRRAVQANDGHLNVRNIPGKGCVFTIDLPR
                     ASGN"
     gene            10734..11123
                     /locus_tag="VMZ53_14690"
     CDS             10734..11123
                     /locus_tag="VMZ53_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS29748.1"
                     /translation="MLDDGPKTTRANRLLLVEDNVEMRELVAEALRSFGFDVEEAGDG
                     VDALAAIRRMRPSLLITDCDMPNMTGNELVRALACDERLCSLPAIVISALKQPPMPDN
                     VVRFLAKPFTLEQLRAAVRDCLVTASL"
     gene            11253..11894
                     /locus_tag="VMZ53_14695"
     CDS             11253..11894
                     /locus_tag="VMZ53_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS29749.1"
                     /translation="MTRPAKILVVDDHPLFRQGLVMALRAQPDFEVAGEAGDSAEALA
                     LIGREVIDVAVVDLLMPNVSGISLAGDLYDRQPGCRVLGLSVIDEPGLIADMLRAHAC
                     GYALKTQPVSEIVEAIRHVLGGCRYLPPTVSANAVDTELATDIVDPLQRLTKREREVF
                     ELLIRGNSNDEIAIRLFIARRTVETHRQRVMGKLSAHSIVKMQRVAARHGGLA"
     gene            12089..13813
                     /locus_tag="VMZ53_14700"
     CDS             12089..13813
                     /locus_tag="VMZ53_14700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS29750.1"
                     /translation="MRLELANNAWRAELAEAPDAIIDVARRVVETNQAIHVPEIAVAR
                     GGRPAYYAATVHPLRGCLGAATGAIVVCAEVTDRVIAQRLGVARAALIWSGVLGGGAD
                     FANGALRSYAGDEWSTAVHADDSEAVATATQQAADEIEIRLRRADGDYRWFRVGFKLG
                     PDGTRWFACASDIHDARAVEREISRLLASARAARAEAEQANRLKDQFLATLSHELRAP
                     LTTMLLWEKLLRDDPQEPIRTQALDAIRQSALAQARMVGDLLDVSRAIAGKLFVDRRP
                     LLLRTVVADALEAASPAALAKGLTLSCSGSRLGHVDGDVARLRQVLDNVLANAVKFTE
                     PGGRIEVMLRRDAGTASIMITDTGRGIAPDYLERIFVPFHQADDLLARRDRGLGLGLA
                     ISRQLVELHGGTLTASSDGLGCGATLKLELPLSRRRRSARLNENVDSAALLRHKRILV
                     IDDDERVRDALSLLLERAGAVVYAADSAAHGREVLETESPDVIVCDIAMPGEDGHSFV
                     RTLRRLRIRIPTIALTAHALPADATLALDAGFDIHLAKPVSFEWLVTSIAALLRRSPH
                     SDRGSAPT"
     gene            13961..14725
                     /locus_tag="VMZ53_14705"
     CDS             13961..14725
                     /locus_tag="VMZ53_14705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS29751.1"
                     /translation="MDFPSTPDDTHFALGTVEMDKLTTEDLDLSSAGMRESEVVQIVH
                     DLKNPLATVSLEMCLLQEKLANSPADVVRAITRVMQNVVYLDRMVQDILDLAAIDTGT
                     FRLGRRPTELRDFLEHVIERSVATRDRPRVFFDAPSTVTMSIDDLRIERVVANLLQNA
                     LKYAPRSTAVVVRLEVSAQSTCVSVIDAGPGLTSNEASYVFDKYRRGPQARTLEGSGL
                     GLYVSRRIVEAHGGCIGVESAQNVGSRFFFELPVTA"
     gene            14722..16083
                     /locus_tag="VMZ53_14710"
     CDS             14722..16083
                     /locus_tag="VMZ53_14710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29752.1"
                     /translation="MIEEEASRTATVVQPGEIVADNYEIVDVLGIGGMGVVYKAVQRS
                     LDRVVAIKLPRPELAADLVVRRRLRCEAIAGARIDHPNIVRVLDFGSHREVPFVVMEH
                     VAGPRLAHLLAEHGPLPVEMVVQIARQIVAGLQDAHDKGVIHADVKCDNVLVETLRDG
                     NLFPRLIDFGIARFVDDGSLRELEDTTVIGTPEYLAPEVIRGEPPGFTADVYAIGIML
                     YELVSGATPFGGGTSATVMMRGLEDDAVPLTLRCPDDVIPTGFEEVVMRSLAKDPADR
                     FADARMLAKALDSIDLSVMPEVTDLTRVRQPPPIFSMEATTATMSAEAITTAALDASE
                     QNPSLGVEGVARDADTIVVSYLELARSLVDDHRLPAAIAELERGIAELMQHGDTPVWR
                     LWLTLAAVYDGVADRTRARVAARQGRECAVRVGAMIGVERADLLLTRLELRHSVTRRE
                     RPW"
     gene            16189..17178
                     /locus_tag="VMZ53_14715"
     CDS             16189..17178
                     /locus_tag="VMZ53_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS29753.1"
                     /translation="MKPLRGRVVVVTGASSGLGRAIAIELAGRGCALVLAARGRDALE
                     AVARQCEELGARTLVVQTDVTRHEEVDALAAATLEAFDRIDVWINNAGVTYYSFLEDG
                     VLELHERVIQTNLLGSVYGARAVVPVFRRQHEGVLINVGSVLSEVGQAFVPSYVISKF
                     GVHGLTEALRVELADERDIHACTIFPFAIDTPHFQSAANAIGKAPRSLPPMQPPEKVA
                     RAVARLIEYPRRTTFVPKVIRLGLLLHMIMPRAVEHLLLDTLRRWHISDRPELPSTGN
                     LYAPGEDQPHGDRPPMISTPRLLAWAAMRFARNQISAVAHRLRPTPKPQLQGT"
     gene            17175..18701
                     /locus_tag="VMZ53_14720"
     CDS             17175..18701
                     /locus_tag="VMZ53_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS29754.1"
                     /translation="MRTKQQYDVVVVGSGPNGLAAAVGLAGSGLSTLVIEAKDTPGGG
                     TRTSELTLPGFLHDVCSSVHPLGIASPWFRALGLDKDVEWIQPPASVAHVLDEQRVVT
                     LERSVDDTAAQMGRDAKAYRALMTPFVERFDELVDMALAPLRFPKAPLLMARFGNDAL
                     RSMRGLARVRFRGDEAPALLGGIAAHAMIPLDNLASASFGLLLGLAGHAAGWPIIRGG
                     SRTLTDALLARLYRAGGELELANPIASMDQLPPARAYVFDVTPRQLLAIAGDRLPSAY
                     RARLSRFRYGPGVYKIDWALRGPVPWRDPRCARSATVHLGGTIEQIARAEQAVHDGHL
                     GAPPFTLFVQPSLFDSSRAPSGMHVGWAYCHVPHGSTTDARDAIENRIERFAPGFKDL
                     ILARSELSPAQLNAYNANYIGGDINGGLSDLRQLFTRPLAKLDPYATGAPDIFLCSSS
                     TPPGGGVHGMCGYYAARSVLANVFARELTAEAGRSQLCAGRSEPRVEGRRKPTTATSG
                     "
     gene            complement(18607..19746)
                     /locus_tag="VMZ53_14725"
     CDS             complement(18607..19746)
                     /locus_tag="VMZ53_14725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS29755.1"
                     /translation="MGVTLHEFTSANRERILERTIDIVAQYADNVPRDELAGSLPQII
                     DELVAALGDGHVAPHDSLRMSPSDAATRQGVLRFELGFDLETVVRCFGALCDSITALA
                     DEERLTFGAHEFRILNQTIDTGIADAVEAFSDQVRHTDNAAATERIGALAHELRNALA
                     AVVVGFDALKDGRAGFASKTATIVSRGLERMETLIDQTLAASKLQADSQLELTRQGLG
                     SIIREVVDMVPASDVRLEVDIDGDATIDADRTLVMSAISNLVQNAVKFTHARGTVTVR
                     ARVNEGDGEAVIEVEDECGGLRVEDPETLFLPFRQRDASRGGAGLGLAIVRDAVVAHG
                     GTVEVRDEAPKGCRFILRWPLSASSDLRRAAHCDPHTTANDQPPR"
     gene            complement(19909..21129)
                     /locus_tag="VMZ53_14730"
     CDS             complement(19909..21129)
                     /locus_tag="VMZ53_14730"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29756.1"
                     /translation="MKAAFVCALALAGCSNKDAATKPRARDAAVISTDAARPPADAAQ
                     ADAPAAPQIRGLFELPAIKGNTDNLFGSELALSDTELFIGESLAGNGRVHVYAFENDA
                     WKKTATLQPGKTGNFGADVDVDGDTLAVSAIEDRQRGKSAGAVFVFERKAGAWKQTAK
                     LVPDKPNYQFGWQLALSGNTLVVGAPWDAGNAHRGSVFVYERVDGTWKLSTTLVAPTR
                     TGTDLFGYDVAVSGDHIAVGSLSAKGAPKDSGAAYVFERGPDGTWPLVSTLVSDDRSA
                     RDSFGNDVALVGDAVIVGAPAHVGGMLRGAVYVFQRKPDGYLQTQRLQPDESQALDYY
                     GDSIAALGDSVVVSAYGTGAAWLYEYRAGTLTQVDYVDGGRGFGSTITLGPDHMAAAM
                     DGRVHGVASFAIKR"
     gene            complement(21126..23105)
                     /locus_tag="VMZ53_14735"
     CDS             complement(21126..23105)
                     /locus_tag="VMZ53_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29757.1"
                     /translation="MGHPFPYVGSGIAAARKAMSCMVCFANPVPKAARAAIEKGAPSP
                     LAGFFDWNDKLLVFGNDDDSLQWVVRATYDHIRDEDDEFEPDPELPSEAMWKEFNAEL
                     DRWLLRVHAKHPIALVIKPIDEEYSTATDAWHDWTCERIPSLVLPLAEKQLDEHSAQY
                     IAEMWRMWIEGQPFDTQQKLVAALAPAEKKALGARKVLVEPKPPVAEIPAPTLEQVDQ
                     IWRALVAQGGSMRLEDAFTAAVIERGLPPTLSLASHLGELFEAKRHSDVIDLARAVLA
                     SGAEFPTNWYPTLADALIATGKLDEAEVVVRQLVGSLESYKPEMLTTAMRYHAARGEH
                     DVEAMLYHAGITYFSTFSYSVPKTQVKQYGPRPSDISARFFAWLERWLSVSVWKPAQM
                     LNVAHWLVWFDSIGVDVCAARVAEFTAERDRRLALYKRLEMAPDEATAREVIDELAPV
                     VSAETAARAFPTFHERAPLAAFDLLDRGIANERREGFRYPTGDRVNAIVCLTYLALNQ
                     PALEGKLAHVYAIARGYVLVKNAGLHFNLACVACRLGMRTEALGHIARSLELGFPNPL
                     QIHDDNDLAPLRGDAVFEKLFVDDVARRERAAKAVLEGEPKKPSKKKPSKKKPSKKKP
                     SKKKPNKKKPAKKPAAKKPAKRKTKTGATSKAKKR"
     gene            complement(23124..>24309)
                     /locus_tag="VMZ53_14740"
     CDS             complement(23124..>24309)
                     /locus_tag="VMZ53_14740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HUS29758.1"
                     /translation="EKRFVPLVLVIAAAVPIVLIAIGTSWQVAILRGVSLIVAASPCA
                     LAISTPSAVLAAVARAARGGVLIKGGAHLESLGKVRAIAFDKTGTLTHGRPKLISVVP
                     ADGITEDDLLATAAGLEALSSHPLAKAIVAGAQERKLVPQVAEQGDAIHGKGIQGKVA
                     GKAVRIGNLAMFEKDAVPAAVSQSAGTLEDKGQTTMIVWREGQFLGVLGVADTLREEA
                     KVALRKLGELGIATTIMLSGDNARVASSIASSVGIREVRAPLLPDGKVAEIRKLARLP
                     GGVAMVGDGINDAPALAAASVGVAMGGTAADATLETADVVLLDDSLTRLPFAVGLARR
                     ASRVIRQNVFIAMGVSGLLVVASTFGWTSIAQAVVLHEGSTLLVVANALLLLRHPLET
                     RS"
BASE COUNT         4145 a         7977 c         7747 g         4440 t
ORIGIN      
        1 cagcatcttc gtcacgattg gcttcgcgtt catgccgggc cacgtcgcag aggcgttctc
       61 ggacaagaag caacccgcga agcaagcgca cgacgccttc gcgcagttcc aagcgaagcg
      121 ggcgacgacg atcaaggccg ggcacgatgc cgcgaaaggg agcgccgcgt ttttgaagct
      181 cgacgccgaa gagcgcgcga cgctccgcga tgcaccggcc gccaagccga agctaaagcc
      241 gttcaacccg ccgaagctcg tcccgaacct gcagtatcgc tgcgaccagg cgcacatgct
      301 gatcccgagc gtgcataccc caggaccgat tgcgtcgttc gagagcgaac gggacatgcg
      361 cgagcagtgc cggccgttcg ccgtcgatcc aaccgacgcc gacaaggtgt gcacgaaccc
      421 cgagctgctt cactgcggaa tcggcgacga gaacaaccgc gaattctgct gcccgaagac
      481 gcatccgatc ttcaacgtct gcgacaagca gtgctatcgc aacaccgact tcaacaacgc
      541 gctcgaggac aaggacgaag gcttccactg cggcacgtcg cgcgattgcg gagcgaccat
      601 caagccatga cgcgcgtcgc actcgccgcg atcgtcgcgc ttgccgcatg ccagccctgg
      661 taccgcgacg ccgagcgtgc cggtcagcgc gccaccgata ccaagtactc gtcgaagctg
      721 atcgccgacg cgaacgccgc gcgcgaccga ggagaccacg ccggtgcggc ttcactcctg
      781 cgccgcgcga tcaccgagat tcccgatcag cccgccgaca cgttcctctc gctcgcgcgc
      841 gaatcgttcg ctgcgaatga catcgccggc ggtcgcgcgg cctgccggcg gggaatggag
      901 ctgtggccca acgatacgag cttccgcgca ttgttgatcg atcagaaggt ccgcgacgat
      961 ctcaccgcac tcgcgatcga agaggccggc cacaagacgt tcagcgatct cgcggctgac
     1021 tcgagcttca ccaccgtcgt ggatgcgttg aagcgtgcga tggacgcaga gcacggtgac
     1081 accgctgccg gcgagctcgc cctgtggctc gcgcattacg gcgttcccga tcacgtcttg
     1141 ctcgtcggtg cgcgggaggc ggccgcggac aaagtctcgc gactcatgga aacgacgccg
     1201 gcgctcgagg ggctccgaac cgctgcggat cgcgccgatg ccgagcttgc ggctggacgc
     1261 ttcgctcgcg cgctcgcgct gtacagcgac gtctatcggc tgctgcccgc ttcggcgatc
     1321 agtgcgcact tcgccaactt cgcgaaggca gcgcagcagg cgaccgatcc agaagcgatc
     1381 gattccggcg catacgcact cgcggtgcaa ggcgacgccg acgcgcaagc tgggaacctc
     1441 gggctcgcca ttcactccta tcgcaaggcc gtggcacgcg caccgtggtg ggccgacgcg
     1501 cgccgcaacc tcgcagcgct ctacgacagc gccggcatga ccgagatggc gaacgccgag
     1561 cgcgcctttg cggatcgcct cggccgctag agcgtcggcg gcgtctcgag cgcgatgacc
     1621 gccgcgaacc gatcggattc ggcgtggcgc tccgtcgcgg tcggatactg ctcgggctcg
     1681 agctggccga gctcacgttc gcagaggcgc gaccattcgg agaaccagcc gagctcggtc
     1741 gacttcgcga ggcacacgtc gtagttcttc acggcggcca ccgcgagtgg atcggccttc
     1801 gtcgtgagca cgtcgcagta cgcttcgatc ttgtcctcgg cgtaggagcc ggagcgcacg
     1861 tccttcggaa tctctgccgt gaacaacgca tcggagaagt tctgcgcgat ttgcgcgagg
     1921 cgcgcgcgag ctgcgatcga gttggccgag tcgttgagct cgagcaccga gacgtacttc
     1981 ttgctgacgg cctcgcctgc tttcgccttc gcggcgaacc acgcgtcgaa ccgcgcgagg
     2041 ctcttgtcgc ggacggcctt gttcttggga tcgaagtcga ggttctgcgg gaacgcgagg
     2101 ccgaggtact tctcgtactc gacgttcgct tcgatcatgc gcgcgagcgc gaggtgatag
     2161 cggacgcctg cagtgtcacc ggttgtcgcc ttggtcgcgt gcttcgcggc ggttgcgagc
     2221 tcggcgaggg cgtcgcggac tttgcgctcg tcacgcggca cgagcgtcag cttgatcttc
     2281 gtgtcgggcc cgcactgcgt aggaagacga gcgagacggc cccgcgtcgc caccgcacgc
     2341 tcgcgcgtga tcttgacgca cgcgtcgtcg acggtcttga cggggcagct gtcctcgaac
     2401 aacagctcgc cgatgcgcgc atgggcgatc acgaccttgt cggcgccgcc tttgtcgccg
     2461 tgaacgcgga tgtattcgcg caggtgcttg atgactgcct cgtggtcacc cttcttctcg
     2521 tagaccgacg tcagggcgaa cgcggcgttc gcgacttcag ctgggttcga cttgccgaac
     2581 gtacgaacga agaacttggt gtcttcgatc gcctttgcgt catcgccggt gcccttgcga
     2641 tagaggaccg cgtcgttcat cgcgtcgtgc gcgtcctcct cggccgcgaa cttcttcgcg
     2701 tactgctcga gccgatccgc cgcgcggtcg tagaacgcga tgtcgccgta cgccttgccg
     2761 agcctcgcga tcgccctcgg cagtaccggt gactgcgggt agtactgctc gagcaacccg
     2821 tacatccgga tcgcgacgcc gatcgagtgg ccgtcctcat agcagacgcc tgcgttgtac
     2881 aggacctcgt cgttcttctt cgcctcggga ttgcggttgt agatcgcgag atacgcgttg
     2941 ccgcactcga cgagctttgc gggatctttc gtcgccctcg cctccttctc gagcttctcg
     3001 gcgatcttgc gcagcgcggt cagctcgatg tcttcggcga cttccttggc ctttggcttg
     3061 tcgtccagaa acttgccgtt cgcgagcagc tcgcgcgctg ctgcgacgag gccgacctcg
     3121 tcgtgcttct ggttgagcga atcgagccag aggttcgcgg cgtcctcggc gaggtcatgg
     3181 gtcgggtact tcgcgacgat gtcgcggaag atcggaatcg cttgatcgaa gcgcgcgtac
     3241 cgccgcagca cgttgccctt cgtgaacttg atgcggacga gctcgtcgtc gttcttggcg
     3301 tccttcactg tcgcgaagta aaggtcgaac gccgcgagca tcttctgatc gcgctcggga
     3361 atctcacccg cttttgccgg agcggcgctg cgcggatcgc gatcatgggc gtagaggaac
     3421 gccaacaccg ccgcgtacgc cgcttctttc gacagcaccg catcgaggcc gccggccttc
     3481 acgacgtccg taaactcgac gccggcgttc tcccacagct gcgtttgcag ccgcgcgtcc
     3541 ttctcggtct ccgcgcgaga ccacaggagc tccgcgaagt agtagtgcgt cgcgggaaag
     3601 tcagacgctt tcgggaacgt cgccaggtac gccttgtaga gcttctccga cgcgctgaac
     3661 gaatcgacgc tcttcgtctt ggtccattcc gagtgatacg cgcgcgcgag ctcgcccgcc
     3721 atcgccgacg cgttgtcgcg acactcgtcg gcatcaccct tcggcagctt gcctgcgacc
     3781 gcgagataca cgcgatcgag atcgacgatc tctttcacct tgtccgcatt cgttgcgccc
     3841 gacagcgcga gcgtcgcgtg tgccacctcg tattgccacg tgcagacgcg cgggctcgtt
     3901 ggctggagct tcatgagctc gcgaagcacg tagatcgcgc ggtcgctctt gccctggccg
     3961 agatagaagt cggcgagtgt ctcgagcatg ccgaatgcag ccttcgtgtc gacgcgcttg
     4021 aatgcatcga atgctttgtc cgctttgccg atttcggcgt acgcacgcac gaagtccttc
     4081 ttcgccgcgc ggttgagtac ttcgtacttc gcgttcgcct tcgtcgcatt cgcgaccttg
     4141 tagaacacct cgagcgcatc ttgatgcttg gcaagattga gatcgatcca gccgagcttg
     4201 tacatcgcgt accagtaggc gttcgagctc gggaactcga gcacgcgctt gtagcgagcc
     4261 tcggcgtcgg cgagctgacc cgactcgaag tagtagtccg cgaacgctag gtgcgcctcg
     4321 ggcacatact tgctcgacgg atagttcttg agcagcttgt cgaagacgga gcgcgcctcc
     4381 ttcacgtact tgccggactg cagcgtgtac gcgtaataga acagcgccag gtccatcttc
     4441 gggaagttgc gaaacgagtc gttgtcggtg aggtccttgt acgtgctgac cgcgccgatc
     4501 atgaacgagt gcgctttgtc cgccgccgct ttcgccgctg ccttgtcgcc ggcaatctcc
     4561 gccctcgcag attgcaggcg gtagacgcgc tgttggctcg cgtacagctc gccgaggcgg
     4621 aagtagtagt ccgccttctc gtcggccgcg gagtcgggcg tcgacttgat gagcttcttc
     4681 aagaggtcga cttgctcctc gcgatacggc acggccgcta gctgcgagtc catgatcgag
     4741 tcgggcgtcg cgacgggttt gggcggcggc gctttgtcct gcgcaaccgg ctttacccgc
     4801 tcggagagct cgacgacggg tggcttcgtg acgcgatccg cgtgtgccag ggccggaagc
     4861 agtgcgcagg cgaggaggac gatgcggtgc atgtcctcga tgacacagcg tcaccgccgg
     4921 tgcagcgtca ccggtccgca aaacgttgtc acggtttcgt atcgcttcgt catcgagacg
     4981 cttgcacgag ctcgccgaac gtcacgttga atggcgagag gatctcgtcg agctctgcgc
     5041 tcgaaccaac gagcaacgcg tgattgacga ggtgatcttc tgccgcgcct tgccgctgca
     5101 ccttcagcgc gttctgcgcg gcggtgagct ccttcgctgc ggtcgcggcg tcgagcggtg
     5161 cgacaactgc atcgacgacc gcagccatcg cggccttgcg cgcttgttcg aacgtacgcg
     5221 tgctcgtgcc atccttgtcg tcgagcgtct cctcgaagtc gacgggtacg gtcttgcctt
     5281 gcaccgtcac cgttccatgc acggtcaccg agaacgagcg gtgtgcgacc tcgacttgca
     5341 cgacctgctc gacatcttcc tcgccgccgt ccttcacgac atgctgcttg atcgtcatcg
     5401 gctcctgcgc tgtccacttc ttctcgggaa tgaccgagca cgcgagcgct gcggtcgcgc
     5461 ggacgcgctt gccggcggtc tccttcacga gcggcgtgca tgcgggcgag agcttgtcga
     5521 atgcgaccga tgtcttcacc gcggcgacaa gcttctcgta ttgctcgaac gcgacgcgcg
     5581 cttcttcgct gctcgaaccc gcgacacgcg cagcaagcgc tgcatagagc cacgctccgc
     5641 cgcggccgcg cgtcgccttg ctgcgaccct tcagatccga gagcgcgcga tcgcggagcc
     5701 cggtcacgag cgccggcaac gcgtgcgacg cgggcagctt cgagaacgca tcggccacgt
     5761 cgacgtagac cgccatcggc gtcgtttgct ggatcgccca cgcaaggaac gagtcccaca
     5821 cgtgaacgag ggccgtcgcg ctcgcctcgt cgacgggaga ccgtgcatcg cgcttgcgaa
     5881 tgagctcgta cgcgatgccc cagttcgcgg aatcgagcgc gaccttgaga tcgtgcgcgg
     5941 ctttcgcgtc cgcatcactc tgcgccttcg cctgctcggc cacagcgttc tgcttcatct
     6001 gcgcgatgcg gatcgcctcg cgctgctgtg acgtcttcgc gccgcgcgtc cgcacgttcg
     6061 cgtcctgctg aatgcggcga atattgtcga gcgcgtctgc aatcgaggct ccgtgcagcg
     6121 gggacgtcgc gagcccgcat gcaggctcac cactgccggt caccgcgtag agcttgcgaa
     6181 cggcatccgc gaatgcgctc gcacgcgccg catccgtgcg ctcgctggcc actttgcatg
     6241 ccgcaccata cgcatcgatg atctccgact gtccccaacc gccgagcttc gcggtcgcga
     6301 catcgcagcc ttcaccgcag cgcttcgcct gttcctccgc gacgagatcg ccgagcacga
     6361 tgcacgcgcg ttggcgctcg atcgcatctc ccgccggatt gccacacatc ccgagcagcg
     6421 cggtgcgcgc acgcgtgcgt ggattcgtcg cgtcgtcgcc ctcgcccttc ggcgcgagca
     6481 cgcgcaaatc ctccgacacc ttgaacgatg cgagcagacc gaccggctcc tcgccgagca
     6541 acaggacgat cgtctgaaag cagcctgctg ctgcatggtc gtgcaagcac gttggcccga
     6601 tcgcatccgt cgccgcttcg acgtattgct tcgccccggc ttcgtctttc gccatcatgc
     6661 gatcgttggc gaccgcgaga cacgcgcccg gatcgccgcc ggccttcgac gtgcacgccg
     6721 ccacttgcgt cttgagcccg gcgccgtgcg agcaagccgc caccgccacc actatcacca
     6781 cgacaatcga acgcagcatc ggccctgcgt atcacgactc ccctagccca aaacagccct
     6841 acttgcggtg ctgcggacgc tctgccaagc cggcggtaag catgttcacc gaatcctcgg
     6901 agagccgcga agattcacgg cttgcggcga cgccatggac aagctcgccg agcgaacgca
     6961 gcgtaaacag cgccagctct tccgccgatt cgccagtcgt actccagatc tctacgacgc
     7021 cgagggctct cgcctgtcct gccgcgggaa tcggcagctc gacgcgaacc cacccgcgca
     7081 ccccgggatc gacgagggcg atgttggcgc cgaccagcgg aatgctcgcg tcgcccggct
     7141 tgccgcgctc accgacctca tcgcacgcct cgatcaccgg tcgatatccg accgcctgca
     7201 accgctcgac gagatcgcca accgttagcg tcttcagctc gcgatacccc tgcggcaccg
     7261 cctcggctgg cctgtacgaa accgtgaagc catcgcgcgt gacatgcgtg ccgcgcgtcg
     7321 ccaacatcgc gacgacgtag ccgacgatca tgccgatcgc gctcgtcatg ccgatgaagc
     7381 gcgccgcgcc gttgcgccag tcgccgagct tgttgacgtc gttcgacgcg aagtgccaac
     7441 cgatcgcaaa cccgatcgcg ccaccgacta gcgcacccac aagcggtccc cagcgcttca
     7501 tggcgggagc gtcctactta cgccgttgcc gcgcaatcgt gttggttacg cacgatgagc
     7561 gtccgcatcg atgcggcagc gcacgcgtct attcaaggtg agtcgagatt cgcaacgcga
     7621 aggatccgcg accggatgta agtccgtggc atgtaagctg cttgggagcg acgccgtgag
     7681 cggcggggtc ctcaaatcac gagcgatgct ggtcgacgat cacccgctct ttcggacggg
     7741 cctcgcgatc acactcgcga aggagccaga ccttcacgtc acggctgacg tcggcagtgc
     7801 cgtcgaagct ctggaggcga tgcgtgcgtc agcaatcgat atcgccgtga tcgatgtctt
     7861 gatgccgacc acgactggcg tctcgctcgc tgccgagctt ctcgagttgc ggccaagctg
     7921 caagatcctc gcgttgtcgg tgctcgacga gccaatgatt atcgcgacaa tgcttcgggc
     7981 cggcgccctt ggttacgcgt tgaaaacgca accaccgaac gagatcacgc aggcaatccg
     8041 catcgtgctt agcggcgctc gatacctacc gccattcgta tcgcacgagg cggtgctcgg
     8101 tctcgtcgcc gatactgtgg agcgtccgat ggatcgcctg acccggcgcg aacgagaggt
     8161 ctttgatctg ttgatccgcg gtcattccaa cgacgagatt gcgacgagac tgttcatttc
     8221 gcgacgcacc gtcgagacgc atcggcaacg aattacgaag aaactggcga cgcactcgat
     8281 cctcgacatg atccgactgg ccgcacgtca aggtgcactg tgcgactgat cattcgatcg
     8341 agttaggcag ccggcggttc gggccgctgc tcttcggcgg accgacgtag agcgacggta
     8401 aacacgcatc cgatgcccgg catatccgtc accatgatgt ccccacctat ggctctcgca
     8461 cctctccggc taatcgccag gccgaggccg actcccgcgc ggttactgtg acgttgtgcg
     8521 tacggcatga agagctcctc gagcttgccg cgaggaaggc cgccgcattg gtctgcaaca
     8581 tcgatgaaga cttggtcatc caggacgcgt acaccgacgg tcacgtggct gtggcgctgc
     8641 gtgaacttgc acgcattctg tacaagattg atgacgatcg agttcacgat ctgcgtgtcg
     8701 ccgtcgaccg ccagccccgg aggtacgagc ttgatcgaga gcatgacgtc ttgagacttt
     8761 gcgtgcgtcg ccgccgcgat ctcgacatgt tcgaaaatct ccgcgagcga cactcgttcg
     8821 tcgcgtggag gtcctgcctc gagccgcacc tccgcgagag ctcgggccac gaggtcgtcc
     8881 attgcggcat ggcttcggac gactaggttc gcagtcgtcc catcaacgcc gacgcttccg
     8941 ctgcgaacgg cctcgaacgc gagcgtcgct gcattgagta ggttcctgag ctcgtgtgcg
     9001 aggaatccga gttgctctac gttacggccg atgattgatt cgtcgctctg tcttgcgaac
     9061 tcggtaactg catctgcgat cgcgatgtcg aggcacaggt tcaacgtttg aaagtctgcg
     9121 ttcgtgatct gcgggccgac ctccaccgcg agctccgtga tcgcttggca gatgttcccg
     9181 tagtactgca ccacctgacc gatggtgtag ccggcgctca gcgattcgct tccgtgaatc
     9241 gaggcactcg cgcccagctg gtcgccaccc tgatccgcac ttacgtccaa ccgacgacta
     9301 agctgatcca ggaaaagaga cactccgtcc aacaaggaga gctcacccgg gcgaggcgtg
     9361 attcggctgg caaccatctc gcgagctcgc gcgatgatta cctctcggta agtcgcgatg
     9421 aactcggaaa gcatgttgca cggtcccaca gtcggtcgtt agtagctcac ccaatcgcga
     9481 tcgcgaccgt gctgtgcggt ttcgctacgt tctgatcgcg cccaaaactc cggcgctgac
     9541 gtggcacgta ttgcgcactc atagaggccg atactcggcg ccaagcgccg taagacgagc
     9601 aggagggcga cgatgctgca cgagtttctg accgcaaatc gcgaagagct tgtaaagcgc
     9661 tgcagggcgc gggtcgccac acgtgccgcc ccgagtgtga cggacgccga gctggagcac
     9721 gggatcccgc tattcctcga ccagctcatt ggcatgctgc ggaaggagct cggaccgccc
     9781 atcgaggctt cgaatcaggc cgcggtgctc accgccaccg cggaaaagca tggaaatgaa
     9841 attttccgga agggattcgc tctcgatcag gtggtccacg actacggcga cctctgccag
     9901 tccgtcaccg agcttgctgt ggagacggac gtgccgatca ccgtcgacga gtttcgcacg
     9961 ttcaatcgat gcctcgacaa cgcaatcgcg gacgcggtca ccgcattcgg agacgtgcgc
    10021 gacaagacga tctccgcaga aggcgcagag gcgatgaatg aacgcctcgg ctcgctcgcg
    10081 cacgagcttc gcaattcgct caatgccgca atcttggcct tcgacgcgat caagaccggc
    10141 agcgtccccg tcacgggcgc gaccggagcg gtactcgatc gcagcctact ggcgttgcgc
    10201 gacgtcatcg atcgctcgct cgcggaggtg cgcttgggtg cgggtctcga tgcgcatcgc
    10261 gaatccatca acgtccgcga gttcgttcag gagctccagg ttgcggcagc gatggacgcg
    10321 aagacgcgag agatcacgtt caacgtctcg ccgatcgaca atcgcctggc ggtcgacacg
    10381 gatcggcaga tgctgggctc cgcaatttcg aacttgctgc aaaacgcgtt caagttttcg
    10441 cggcacaacg gcagcgtgtt gctgaccgcg cgcgccgtcg acgatcgagt catcctcgag
    10501 gtcgaagacg agtgtggcgg tctgcctgcg ggcaaggccg aggagctgtt tcggccgttc
    10561 gaacagaaga gcggagaccg aaccggtctt ggtctaggac tgtcgatcag ccgccgcgcg
    10621 gtccaggcga acgatggcca cctcaacgtg cgcaacatcc ccgggaaagg ttgcgtcttc
    10681 acgatcgacc ttcctcgcgc gagtgggaac tagcacgcgt ggcgggcgcg aacgtgctcg
    10741 acgacgggcc gaagaccacc agggcgaatc gtttgctgct ggtcgaggac aatgtggaga
    10801 tgcgagagct tgttgcagag gcgctgcgca gctttggctt cgacgtcgaa gaggcaggcg
    10861 atggagtaga tgcactcgcg gcaatcaggc gcatgcgtcc gtcgctgctc atcaccgact
    10921 gcgacatgcc aaacatgacg gggaacgagc tagtccgagc cctcgcctgc gacgaacggc
    10981 tgtgttcgct ccctgcgatc gtcatctctg cattgaagca accgccaatg ccggacaacg
    11041 tggtcaggtt tctggcgaag ccattcacac tggagcagct ccgcgcagcg gtccgcgatt
    11101 gtctcgtgac cgcaagtctc taactgcgcg cttcctacgt gctggatatt tcctcgcgcg
    11161 aggaattgcg tcgaggtttt attgcccctc cgttcgattc gtcaaaacag atcgatctca
    11221 tcgcgtcgag acgtgccatg catgaaacgc gcatgactcg accagcgaag atactcgtcg
    11281 tcgacgacca tccattgttc cgacagggac tcgtgatggc cctgcgcgcg cagcccgatt
    11341 tcgaggtcgc cggtgaagca ggagacagcg ccgaagcgct ggcgctcatc ggccgcgaag
    11401 tgatcgacgt cgcggtcgtt gacctgctca tgccaaacgt ctccgggatt agcctcgcgg
    11461 gagacttgta cgaccggcaa ccagggtgtc gcgtgctcgg cttgtctgtg atcgacgagc
    11521 ccggcttgat cgcggacatg ctgcgtgcgc acgcgtgcgg atatgcgctc aagacgcagc
    11581 ccgtcagcga gatcgttgaa gcgattcgtc acgtgctcgg tgggtgccgc tacttgccgc
    11641 ctacggtgtc ggccaacgcc gtcgacaccg agctcgcgac cgacatcgtc gatccattgc
    11701 agcggctgac gaagcgcgag cgtgaggtgt tcgagctatt gatccgaggc aactcaaacg
    11761 acgagattgc gattcgtctg ttcattgcgc ggcgcaccgt cgaaacacac cgccagcgcg
    11821 tcatgggcaa gttgtccgca cactcgatcg tgaagatgca acgcgtcgcc gctcgccacg
    11881 gcggcctggc ttgatgctag atgcgctgac ctcggacgca gcgcgcgagt ggcaacttga
    11941 agtggcgtgc tcgctcatgc acggcctcgc cgagctattg ctgccgagcc cgcccgtggg
    12001 gatcgtggag gagcgcgtgc ggcgccgcaa cgctctgcga atgctagatc agttcgcgct
    12061 gcctgctgcc gtttacagtc acgcaggggt gcgcttggag cttgcgaaca acgcgtggcg
    12121 agccgagctc gcggaggcgc cggatgcgat catcgacgtg gctcgccgcg tagtcgaaac
    12181 gaatcaggct attcatgtgc cggagattgc tgtcgcacgt ggaggccgcc cggcctacta
    12241 cgccgcgact gtgcatccgt tgcgcggctg tctcggtgca gcgaccggcg cgatcgtcgt
    12301 gtgtgcagag gtgaccgacc gcgtgatcgc gcagaggctc ggcgtcgcac gcgcggcgct
    12361 gatctggagt ggcgtgctcg ggggtggagc cgacttcgcg aacggcgcgc tgcgttctta
    12421 cgccggcgac gagtggtcca ccgcggtgca cgccgacgac agtgaagcag tcgccacagc
    12481 aacacagcaa gcggcagacg agatcgagat tcgcctgcga cgcgcggatg gcgactaccg
    12541 ttggtttcgc gtcggattca agctgggccc ggatgggacg cgttggtttg catgcgcgag
    12601 cgacatccat gacgcccgtg ccgtcgaacg cgaaatctcg agactgttgg cgagcgcgcg
    12661 agcagcgcgc gcagaggcgg agcaagcgaa tcggctcaag gatcaatttc tcgccacgct
    12721 gtcccacgag ctacgtgcac cgctcacgac gatgttgttg tgggaaaaac tgctgcgcga
    12781 tgacccgcaa gagccgatca gaacgcaagc gttggacgcg atccgccaga gcgcgctcgc
    12841 gcaggcacga atggttggtg atctgctcga cgtgtcgcgc gcgatcgctg ggaagctgtt
    12901 tgtcgatagg cggccgctcc tcctcagaac cgtggtcgcg gacgccctcg aggccgcgtc
    12961 gccggcagcg ctcgcgaagg gcctgacact gtcgtgttcg gggagccgac tgggccatgt
    13021 cgacggcgac gtcgcgcggc tgcgccaggt gctcgacaac gtcctcgcca acgcggtcaa
    13081 gttcacggag cccggtggtc gcatcgaagt gatgctccgc cgcgacgccg gcaccgcctc
    13141 gatcatgatc accgacacag gtcgcgggat cgcgcccgac tacctcgagc gcatcttcgt
    13201 accgtttcat caagctgatg acctgttggc gcgccgagac cgtggcctcg gactcggcct
    13261 tgcaatctca cgccaactcg tcgagctgca tggaggcacg ctgaccgcct ctagcgacgg
    13321 actcggctgt ggcgcgacgc tgaagctaga gttgccactg tctcgcagac ggcggagcgc
    13381 tcgcttgaac gagaatgtcg acagcgcggc gcttctgcgg cacaagcgta ttcttgtcat
    13441 cgacgacgac gagcgcgtac gcgatgcgct ctcgctcctg ctcgagcgag caggtgctgt
    13501 ggtctatgcg gcggactcgg cagcgcacgg ccgtgaggtt ctcgaaaccg aatcgccgga
    13561 tgtaatcgtt tgtgacattg cgatgccagg cgaggacgga cattcgttcg tacgtacact
    13621 gcgccgcttg cggatccgga ttccgacaat cgcgctcacg gcgcacgcac tcccggctga
    13681 cgccacgctc gctctcgacg cggggttcga tatccacctc gcaaagcccg tgagcttcga
    13741 atggctcgtc acgagcatcg cagctcttct acgtagatct ccgcatagtg atcgcggatc
    13801 agcaccgacg taacggagcc gtaacgagcg catcgaagat tacgtaaaca accgcatggt
    13861 cacgcggcgt cgtcttgcgc atcttgttcg tgaacggagg attctcatgc gacgcgagga
    13921 tggtcagaca cgacaggccg acgcactccg acacgctgca atggacttcc catcaactcc
    13981 ggatgacacg cactttgcgc tcggcaccgt cgagatggac aagctgacga cggaggatct
    14041 cgatctctcg tcggctggga tgcgcgagtc tgaggtcgtg cagatcgtcc acgatctgaa
    14101 gaatccactc gcgacggtga gcctcgaaat gtgcctgctg caggaaaagc tcgcgaattc
    14161 acctgccgac gtagtccgcg cgatcacgcg tgtgatgcag aacgtcgttt acctagatcg
    14221 catggtgcag gacatcctcg accttgcggc gatcgacacc ggcaccttcc ggctgggccg
    14281 gcgaccgacc gagctgcgtg attttctcga gcacgtgatc gaacgctcag tcgcaacgcg
    14341 cgaccgcccg cgtgtcttct tcgacgcgcc gtccactgtc acgatgtcga tcgatgacct
    14401 tcgcatcgag cgagtggtcg caaatctcct gcagaacgct ctcaaatacg cgccccgctc
    14461 gacggctgtg gtggttcgcc tagaagtaag cgcacagtcg acgtgcgtat cggtgatcga
    14521 tgcggggccc gggttgacga gcaacgaggc cagctacgtc ttcgacaagt atcggcgcgg
    14581 tccgcaggcg cgcacgctcg aaggcagtgg gctcggtttg tacgtgtcga ggcgcatcgt
    14641 cgaggcgcac ggtggctgca ttggagtcga gagcgcccaa aacgtcgggt cacgtttctt
    14701 tttcgagctg ccggtaacgg catgatcgaa gaggaggcgt cccggaccgc gacggtcgtg
    14761 cagcccggcg aaatcgtcgc ggacaattac gagatcgtcg acgtcctcgg catcggcggc
    14821 atgggcgtcg tgtacaaggc cgtacagcgc tcgctcgatc gcgttgtcgc gatcaagctc
    14881 ccgcgcccag agctggcagc cgatctcgtc gtgcgccggc ggctgcgttg cgaggcgatc
    14941 gccggtgcgc gcatcgacca ccccaacatc gtccgcgtgc tcgacttcgg cagtcatcgt
    15001 gaagtgcctt tcgtcgtcat ggagcacgtc gccggtccgc ggctcgcaca tctgctcgcc
    15061 gaacacgggc ctttacccgt cgaaatggtg gttcagatcg cgcggcaaat cgtcgccggc
    15121 ctacaagacg cacatgacaa gggcgtaatc cacgcagacg tgaaatgcga caacgtgctc
    15181 gtcgagacgt tgcgcgacgg caacctattt ccgcgcctga tcgattttgg gatcgcccgt
    15241 ttcgtcgacg atggctcgct ccgcgagctc gaggacacga cggtcatcgg gacgccggag
    15301 tacctcgcgc cggaagtgat ccgcggcgag ccaccgggat ttactgccga tgtctatgcc
    15361 atcggcatca tgctctacga gcttgtgagc ggtgcaacac cgttcggtgg tggcacgagc
    15421 gcgaccgtga tgatgcgcgg cctcgaggac gacgcagtgc cgctcacatt gcgctgcccc
    15481 gacgacgtga ttccaactgg gttcgaagaa gttgtaatgc gctccctggc gaaagatccg
    15541 gctgaccgat ttgccgatgc gcggatgctg gcaaaggccc tggactcgat cgacctcagc
    15601 gtgatgcccg aggtcacaga tctcacacgc gtcaggcagc ccccaccgat cttctcgatg
    15661 gaggcgacga cggctacgat gagcgctgag gcgatcacca ccgctgctct cgacgcgtcc
    15721 gagcaaaatc cgtccctcgg agtggagggt gtggcacgtg acgccgacac gatcgtcgtc
    15781 agctatctgg agctcgcacg ttcgcttgtc gacgatcatc gcctccctgc cgcgatcgcc
    15841 gagctcgagc gcgggatcgc agagctcatg caacatggcg acacgccggt gtggcggttg
    15901 tggctgacgt tggccgcggt ctacgacgga gttgcggacc gtacgcgtgc acgagttgcg
    15961 gcgagacagg gtcgagagtg tgctgttcgc gtaggcgcta tgatcggggt cgagcgtgcg
    16021 gacctactgc tgacgcgact cgagctccgt cactcagtca ctcgtcgcga gcgaccgtgg
    16081 tagccacacg gcagcggtga gtcgacgcgc tacgatctcg gacgtgcgtg ggcgctcggg
    16141 ggcgaggctc gacggtaacg aggcacggcg gctgcacgat ttcacggcat gaagccacta
    16201 cgagggcgcg ttgttgtcgt caccggcgcg tcgagtggcc tcgggcgtgc gatcgccatc
    16261 gagctggcag ggcgagggtg cgcgctcgtc ctcgccgcac gtgggcgcga cgcgctcgaa
    16321 gcagtggcgc ggcagtgcga agagctcggg gctcggacgc tcgtcgtgca gaccgatgtg
    16381 acgcggcacg aggaagtcga tgcgctcgcg gcggcgactc tcgaggcgtt cgaccgcatc
    16441 gatgtctgga tcaacaacgc gggagtcacc tactactcgt ttctcgagga cggggtactc
    16501 gagctgcacg agcgggtgat ccagacgaac ttgctcgggt cggtgtacgg cgcacgagcg
    16561 gtcgtgcccg tgttccggcg ccagcacgaa ggcgtgctca tcaacgttgg ctccgtgctg
    16621 agtgaagttg gtcaggcatt cgtgccgtcg tacgtcatca gcaagttcgg cgtccacggg
    16681 ctcaccgagg ccttgcgcgt cgagctcgcc gacgagcgcg atatccatgc gtgcacgatc
    16741 ttcccgttcg cgatcgacac gccgcatttc cagagtgcgg caaatgcgat cggcaaggca
    16801 ccacgctcgc tgccgccgat gcagccgccg gagaaagtgg cgcgcgcggt ggcgaggctg
    16861 atcgaatatc cgcgccgcac gacgttcgtg ccgaaggtga tccgacttgg tttgctgctg
    16921 cacatgatca tgccgcgcgc cgtcgagcat ctcctgctcg atacgctgcg gcgctggcac
    16981 atcagcgaca gacccgagct gccgtcgacg ggaaacctct acgcgcctgg cgaagatcag
    17041 ccgcacggcg atcgcccacc gatgatctcg acgccgcgcc tgctggcgtg ggcagcgatg
    17101 aggttcgcaa ggaatcagat tagcgcggtg gcgcaccgac tgcgtccgac gccgaagccg
    17161 cagctccagg gaacatgagg acgaagcagc aatacgacgt cgtcgtggtc ggctcgggac
    17221 cgaacgggct cgcggccgct gtcggcttgg ccggctcggg gctctcgacg ctcgtgatcg
    17281 aagcgaagga cacgccgggt ggaggcacgc gaacgagcga gctgacgctg cccggctttc
    17341 tgcacgacgt ctgctcgagc gtgcatccgc tcggcatcgc atcgccatgg tttcgcgcgc
    17401 tcggcctcga taaggacgtc gagtggattc agccgcccgc gtcggtggcg catgtcctcg
    17461 acgagcaacg tgtcgtcacg ctggagcgat cggtagacga cacggctgcg cagatgggac
    17521 gcgacgcgaa ggcgtatcgc gcgctgatga ctccgttcgt cgagcgcttc gacgagctcg
    17581 tggacatggc gctcgcaccg ctgcgctttc ccaaagcgcc gctactcatg gcgcggttcg
    17641 gcaacgacgc gttgcggtcg atgcgaggcc tcgcacgggt ccgcttccgc ggcgacgaag
    17701 ctcctgccct gctcggcggc atcgccgctc acgcgatgat tccgctcgat aacctcgcga
    17761 gtgcgtcgtt cggcctgctg ctcggcctcg cgggacatgc ggccggctgg ccaatcatcc
    17821 gcggcggcag tcggaccctc acggatgccc tgctcgctcg cttgtatcgc gcaggcggcg
    17881 agctcgagct ggcgaacccc atcgcgtcga tggatcagct tccgcccgca cgcgcgtacg
    17941 tcttcgacgt gacgcctcgc cagttgctcg cgatcgcggg tgaccggttg ccctcggcat
    18001 atcgcgcgcg gctgtcccgg tttcggtatg gccccggcgt ctacaagatc gattgggcgt
    18061 tgcgcggccc cgtgccgtgg cgcgacccac gctgcgcgcg ttcggcgacc gttcatcttg
    18121 gaggcaccat cgagcaaatc gcgcgagcgg agcaagcggt ccacgacggc catctgggcg
    18181 cgcccccttt cacgctgttc gtacaaccct cgctgttcga ttcatcgcgc gcgccaagcg
    18241 gcatgcacgt cggttgggcg tactgccacg tcccgcacgg ctcgacgacc gacgcccgag
    18301 acgcgatcga gaatcgcatc gagcggtttg ccccgggctt caaggatttg atcctcgcgc
    18361 gctcggagct gagccccgcg cagctgaacg cgtacaacgc caactatatc ggcggcgaca
    18421 tcaacggcgg cctctccgac ctgcggcagc tgttcacccg gccgctcgcg aagctcgacc
    18481 catacgcgac cggcgcgccc gatatcttcc tctgctcgtc gtcgactccg cccggtggcg
    18541 gcgttcacgg catgtgcggc tactacgcgg cgcgcagcgt gctcgcgaac gtgttcgcac
    18601 gcgagctcac cgcggaggct ggtcgttcgc agttgtgtgc gggtcgcagt gagccgcgcg
    18661 tcgaaggtcg gaggaagccg acaacggcca cctcaggatg aagcgacaac ccttgggtgc
    18721 ttcgtcgcgg acttctaccg tgcctccgtg ggcgacgacc gcgtcgcgaa cgatcgcgag
    18781 ccctagcccg gcgccacctc gagatgcgtc acgctgacga aaaggcagga acagagtctc
    18841 cgggtcttct acccggaggc cgccgcactc gtcttctact tcgatgactg cttcgccgtc
    18901 gccttcgtta acgcgtgcgc ggaccgtaac ggttccgcgt gcgtgagtga acttgacagc
    18961 gttctggacg agattgctga tggcagacat caccaacgta cggtcagcat cgatggtcgc
    19021 gtcgccgtcg atgtcgacct cgagtcgaac gtcggaggca ggcaccatgt ccacgacctc
    19081 gcggatgatc gatcctagcc cttgacgagt gagctcgagc tgggaatccg cttgcagctt
    19141 cgacgcagcg agcgtttggt cgatgagcgt ctccatgcgc tcgagacccc ggctcacgat
    19201 cgtcgccgtc ttacttgcga acccagcgcg gccgtccttc aaggcatcaa aaccgacgac
    19261 aaccgcagcc aaggcgttac gcagctcgtg cgccagcgcg ccgatgcgtt cggtcgctgc
    19321 cgcattgtcg gtgtggcgga cctggtcgga aaacgcctcg acggcatcgg cgatcccagt
    19381 gtcgatcgtt tgattcaaaa ttcgaaactc gtgcgcgccg aacgtgagtc gctcctcgtc
    19441 ggcgagcgcc gtgatcgagt cgcagagagc gccgaagcat cgaacgaccg tttcaaggtc
    19501 aaagcccaac tcaaagcgca acacgccctg ccgcgtcgct gcgtcactcg gtgacatccg
    19561 aagtgaatcg tgcggagcta catgaccatc ccctaacgcg gccaccagct catcgataat
    19621 ttgcggcaac gaaccggcga gctcatcgcg cggcacgttg tcggcgtact gcgcgacgat
    19681 gtctatcgtc cgctcgagga tgcgctcgcg attcgcgctc gtgaactcat gcagggtgac
    19741 gcccataacc ctccgtcctc ggcattcgtg ctcacaggtt ttgcatgcgt catgccgcgc
    19801 gtgccgcacg ccgagtttcg aaaggcctgc ctatccaccg tcggattgcg gacgcgatga
    19861 acgcgtctgc gatcaaaacg tgtcctaggc gcactccgat gaccgtcgct accgcttgat
    19921 cgcgaacgac gcgacgccgt gcacccgacc gtccatggcc gcggccatgt gatcgggacc
    19981 taacgtgatc gtcgacccga atccgcgacc gccgtcgaca tagtcgacct gtgtcagcgt
    20041 tcccgcacgg tactcgtaga gccacgcggc gccagtgccg tacgcggaga ccacgacgct
    20101 gtcaccaagc gccgcgatcg agtcgccgta gtaatcgagc gcttgcgact cgtcgggttg
    20161 caacctctgc gtctggagat atccgtcggg cttgcgctgg aagacgtaga ccgcgccgcg
    20221 aagcatgccg ccgacgtgcg ccggagcgcc gacgatcacc gcatctccga cgagtgccac
    20281 gtcgttgccg aacgagtctc gcgccgagcg atcgtcggag acaagcgtcg agacgagcgg
    20341 ccaggtgccg tcggggccgc gctcgaacac gtatgcagca cccgagtctt tcggcgcgcc
    20401 cttcgccgag agcgagccga ccgcaatatg gtcaccgctg accgcgacgt cgtagccgaa
    20461 caggtccgtc cccgttcgcg tgggcgcaac cagcgtggtc gagagcttcc acgtgccgtc
    20521 cacgcgctcg tagacgaaca cgctgccgcg atgtgcattg cccgcatccc acggcgctcc
    20581 gacgacgagc gtgttgccgg acagcgcgag ctgccagccg aattgatagt tcggcttgtc
    20641 cgggacgagc ttcgcagtct gcttccacgc gccggctttg cgctcgaaga cgaacacggc
    20701 acctgcgctc tttcctcgct gacggtcttc gatcgcgctc accgcgagcg tgtcaccgtc
    20761 gacgtcgacg tcggcgccga agttgccggt cttgcccggc tgcagcgtcg cagtcttctt
    20821 ccacgcatcg ttctcgaacg cgtacacgtg gacgcggccg ttgccggcaa gcgactcgcc
    20881 gatgaacagc tcggtgtcgg agagcgcgag ctcgctaccg aacaggttgt cggtgttgcc
    20941 cttgatcgcc ggcagctcga agagcccgcg gatctgtggc gctgccggtg catcggcctg
    21001 ggctgcatcg gcgggcggac gagccgcgtc tgtcgatatg acagcggcat cacgcgcacg
    21061 cggcttcgtg gcggcgtctt tgttcgagca gccggcgagc gcgagagcac aaacgaacgc
    21121 ggctttcatc gcttcttcgc cttcgacgtg gccccggtct tcgtcttgcg ctttgctggt
    21181 ttcttcgccg cgggcttctt cgccggcttc ttcttgttcg gcttcttctt gctcggcttc
    21241 ttcttgctcg gcttcttctt gctcggcttc ttcttgctcg gcttcttcgg ctcgccttcg
    21301 agcacagcct tcgcggctcg ttctcgacgt gctacatcgt cgacgaacag cttctcgaag
    21361 accgcatctc cgcgcagcgg cgcgaggtca ttgtcgtcgt ggatctggag cggattcggg
    21421 aacccgagct cgagcgagcg cgcgatgtgg ccgagtgcct cggtccgcat cccgaggcgg
    21481 cacgcgacgc acgcgaggtt gaagtgcagg cccgcattct tgacgagcac gtagccgcgc
    21541 gcgatcgcgt agacgtgcgc gagcttgccc tcgagcgccg gctgattgag cgcgagatac
    21601 gtgaggcaca cgatcgcatt gacgcgatca cccgtcggat agcgaaagcc ctcacggcgt
    21661 tcgttcgcga tcccgcgatc gagcaggtcg aaggcggcga gcggcgcgcg ttcgtgaaat
    21721 gtcggaaacg cgcgagctgc agtctccgcg ctgacgaccg gcgcgagctc gtcgatgacc
    21781 tcgcgcgctg tcgcctcgtc cggcgccatc tcgagccgct tgtagagagc gaggcggcgg
    21841 tctcgctccg ccgtgaattc ggcaacgcgc gctgcgcaga cgtcgacgcc gatcgaatcg
    21901 aaccacacga gccaatgcgc gacgttgagc atctgcgccg gcttccacac gctcacggac
    21961 agccagcgct cgagccatgc gaagaacctc gcgctgatgt ccgatgggcg cggcccatac
    22021 tgtttcacct gcgtcttggg cacgctgtac gagaacgtcg agaagtacgt gatgcccgcg
    22081 tgatagagca tcgcttcgac gtcgtgctcg ccgcgcgccg cgtgatagcg catcgcggtg
    22141 gtcagcatct ctggcttgta cgactcgagc gagccgacga gttgccgcac gaccacctcg
    22201 gcttcgtcga gcttgcccgt tgcgatcaat gcatccgcga gcgtggggta ccagttcgtc
    22261 gggaattcgg cgccggatgc cagcacggcg cgcgcgaggt cgatgacatc gctgtgccgc
    22321 ttcgcctcga acaactcgcc gaggtgcgac gcgagcgaca gcgtcggcgg aagcccgcgc
    22381 tcgatcacgg cggcggtgaa cgcatcttcg agccgcatcg agccgccttg cgcgacgagc
    22441 gcgcgccaga tctggtcgac ctgctcgagc gtcggggcgg gaatctcggc gaccggcggc
    22501 ttcggctcga caagcacctt gcgcgcgccg agcgccttct tctccgcggg cgctagcgca
    22561 gcaacgagct tctgctgcgt atcgaacggc tgcccctcga tccacatgcg ccacatctcg
    22621 gcgatgtatt gcgcgctgtg ctcgtcgagc tgcttctccg cgagcggtag cacgaggctt
    22681 ggaatgcgct cgcaggtcca gtcgtgccac gcgtcggtgg ccgtcgagta ctcctcgtcg
    22741 atcggcttga tgacgagcgc gatcggatgc ttcgcatgca cgcggaggag ccagcgatcg
    22801 agctcggcgt tgaattcctt ccacatcgcc tcggacggca gctcgggatc cggctcgaat
    22861 tcgtcgtcct cgtcgcggat gtgatcgtac gtcgcgcgga caacccactg cagcgagtcg
    22921 tcgtcgttgc cgaacacgag cagcttgtcg ttccagtcga agaagcctgc gagcggcgac
    22981 ggcgcgccct tctcgatcgc tgcgcgcgcg gccttcggca ccggattcgc gaagcagacc
    23041 atgcacgaca tcgccttgcg cgccgccgcg atcccactac cgacgtacgg aaatggatgc
    23101 cccaccggcc gtagagtaac agcctagctg cgcgtttcga gcgggtggcg caggagcagc
    23161 agcgcgtttg cgacgacgag gagcgtactg ccctcgtgga gcaccacggc ttgcgcgatg
    23221 ctggtccagc cgaacgtcga tgcgacgacg agcaggccgc tcacgcccat cgcaatgaac
    23281 acgttctggc gaatcacgcg cgacgcgcga cgggcgagac cgacagcgaa cggcagtcgc
    23341 gtgagcgaat cgtcgaggag cacgacgtcc gcagtctcga gcgtcgcgtc tgcggccgtg
    23401 ccacccatcg caacaccgac ggatgcggca gcgagcgcgg gcgcatcgtt gatgccgtcg
    23461 ccgaccatcg cgacaccgcc gggcaggcgc gccagcttgc ggatctcggc gaccttgccg
    23521 tcggggagca gcggcgcgcg cacttcgcga atgccgacgc tcgatgcgat cgagctggcg
    23581 acgcgcgcgt tgtcgcccga gagcatgatc gtcgtggcga tgccgagctc gcccagcttg
    23641 cggagggcga ccttcgcttc ttcgcggagt gtatcggcga cgcccagcac gccgaggaac
    23701 tgcccctcgc gccacacgat catcgtcgtc tggcctttat cttcgagcgt gccggcggac
    23761 tgcgaaacgg cggccgggac ggcgtccttc tcgaacatcg cgagattgcc gatgcgcacg
    23821 gctttgccgg cgaccttgcc ttggatgccc ttgccgtgaa tcgcgtcgcc ctgctctgcc
    23881 acctgcggaa cgagcttcct ctcctgcgcg ccggcgacga tcgccttggc gagcggatgg
    23941 ctcgacagcg cttcgaggcc ggcggcggtc gcgagcaagt cgtcctcggt gatgccgtcg
    24001 gcggggacca ccgagatgag cttcggccgg ccgtgcgtca gcgtgccggt cttgtcgaat
    24061 gcgatcgcac ggaccttgcc gagcgattcg aggtgcgcgc cacctttgat tagcacgcca
    24121 ccgcgcgccg cgcgagccac cgcagcgagc accgcgctcg gcgtcgagat ggcgagtgcg
    24181 catggcgacg cggcgacgat cagcgacacg ccccggagga ttgcaacttg ccagctcgtc
    24241 ccgatcgcga tgagcacgat gggcacggca gccgcgatca cgagcacgag cggcacgaag
    24301 cgcttctcg
//
LOCUS       DATLBZ010000279         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4020155, whole genome shotgun sequence.
ACCESSION   DATLBZ010000279 DATLBZ010000000
VERSION     DATLBZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4020155"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2085)
                     /locus_tag="VMZ53_14745"
     CDS             complement(<1..>2085)
                     /locus_tag="VMZ53_14745"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUS29759.1"
                     /translation="ARERSNDRQHISLFREKGAQSLRDQRRDPDDENDLKIAVARERK
                     IWMHEVLTKLGTERSRHWGWTNTYTYTKSLGEQIILSDPTVPVTIVRPAVVESAVRYP
                     FPGWNEGFNTTAPLMYLVLKGHRNVPMGDDTALDVIPVDFIATGMLLATAAILADQHE
                     PVYQLGSSDVNRITSKRLTELTSLAVRRHFREKSEDPNESGTEKLRYKLRARLEGKAV
                     TYEYFEKWSAPRFKRIADRLMTLIDEKMPKWGAPRLEAFGERARDELQKVSTFTGQIN
                     ELVDLFKPFTNDHDVAFRCDNIRALWARLTPVDQEKLLWAPHLINWREYWMNTHFPGL
                     QKWTFDKLDEEFGAKPKSVYTYKELVEMFEATVKLHRNRTALRLLRKEEGVDPIAYTY
                     GHMSELAWQGAGMLRQLGVKAGDRVMLMSENRPEWGISYFAIILAGATVVPLDKELTM
                     PEVQNLAKVSKAKVIVFSRKVAERLAGEANLAIPVGSDEDDITVVWSPAHTALHEWFG
                     KQLDTSPRVVAYDELLTEPDVSVGAVQPEVKGDSVASLIFTSGTTGQPKGVMLTHKNL
                     TSMVSKLSSLFTLYKHDKLLSVLPLHHTFEFSAGFLMPLLHGASIDYLEEIEADSLAR
                     ALEDEGVTGMVGVPALWQLLERKIYKNVSDAGVLVEKAFDSIVDLNRSLRDKIPWDLG
                     TGKLLFFPVHRKL"
BASE COUNT          332 a          675 c          667 g          411 t
ORIGIN      
        1 gagctttctg tgcacgggga agaacagcag cttgcccgtg ccgaggtccc acggaatctt
       61 gtcgcggagt gagcgattga gatcgacgat cgaatcgaac gccttctcga cgagcacgcc
      121 tgcatcgctg acgttcttgt agatcttgcg ctcgagcagc tgccacagcg ccggcacgcc
      181 gaccatgccc gtgacgcctt cgtcttcgag cgcgcgcgcg agcgagtccg cctcgatctc
      241 ctcgaggtag tcgatcgatg cgccgtggag cagcggcatc aggaagcccg cggagaactc
      301 gaacgtgtga tggagcggca ggacgctcag cagcttgtcg tgcttgtaga gcgtgaacag
      361 cgacgacagc ttgctcacca tcgacgtgag gttcttgtgc gtgagcatca cgcccttggg
      421 ctgccccgtc gtgccgctcg taaagatcag cgacgcgacc gaatcaccct tcacctcggg
      481 ctgcactgcg ccgaccgaca catcgggctc cgtcagcagc tcgtcgtacg ccaccacgcg
      541 cggcgacgta tctagctgct taccgaacca ctcatggagc gccgtgtgcg cgggcgacca
      601 gacaacggtg atgtcgtcct cgtcgcttcc caccggaatc gccaggttcg cttcgcccgc
      661 aagccgctcg gcgaccttgc gcgagaacac gattaccttc gccttgctga ccttcgcgag
      721 gttctggacc tcgggcatcg tgagctcttt gtcgagcggt acgaccgtcg cgcccgcgag
      781 gatgatcgcg aagtacgaga tgccccactc cgggcggttc tcgctcatca gcatcacgcg
      841 gtcgccggcc ttgacgccga gctggcgcag catgcccgcg ccttgccacg cgagctcgct
      901 catgtggccg tacgtgtacg cgatcgggtc gacgccctct tctttgcgca ggagacgcag
      961 cgcggttcga ttgcggtgga gcttcaccgt cgcctcgaac atctcgacga gctctttgta
     1021 cgtgtagacg ctcttcggct tcgcgccgaa ctcttcgtcg agcttgtcga acgtccactt
     1081 ctggagaccc gggaagtgcg tgttcatcca gtactcgcgc caattgatca ggtgcggcgc
     1141 ccagaggagc ttctcctgat cgacgggcgt gagccgcgcc cacagcgcgc ggatgttgtc
     1201 gcagcggaat gcgacgtcgt gatcgttggt gaacggcttg aacagatcga cgagctcgtt
     1261 gatctggccg gtgaacgtcg aaactttctg cagctcgtcg cgagcgcgct caccgaatgc
     1321 ctcgaggcgc ggcgcgcccc acttcggcat cttctcgtcg atcagcgtca tcaaccgatc
     1381 ggcgatgcgc ttgaaccgcg gcgcggacca cttctcgaag tactcgtacg tgacggcctt
     1441 gccctcgagg cgcgcgcgca gcttgtagcg cagcttctcc gtgccggact cgttcggatc
     1501 ttccgacttc tcgcgaaagt gccggcgcac cgccagcgag gtcagctcgg tgaggcgctt
     1561 gctcgtgatg cgattcacgt cgctcgagcc gagctgatac acgggctcgt gctggtccgc
     1621 gaggatggcg gccgtcgcga gcagcatgcc ggtggcgatg aagtcgacgg ggatgacgtc
     1681 gagcgccgtg tcgtcgccca tcgggacgtt gcggtgtccc ttgagcacga gatacatgag
     1741 cggcgcggtc gtgttgaagc cttcgttcca acccgggaac ggatagcgca ccgcactctc
     1801 gacgaccgcg ggtcgcacga tcgtgaccgg cactgtcggg tcgctcagga tgatctgctc
     1861 gccgaggctc ttcgtgtacg tgtacgtgtt cgtccagccc cagtgacgcg agcgctccgt
     1921 gccgagcttc gtcaggacct cgtgcatcca gatcttgcgc tcgcgggcga cggcgatctt
     1981 gaggtcgttc tcgtcgtccg gatcgcggcg ttggtcgcgc agtgactggg cgcccttctc
     2041 gcggaatagc gagatgtgct ggcggtcgtt cgaacgctcg cgcgc
//
LOCUS       DATLBZ010000280        19163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8350071, whole genome shotgun sequence.
ACCESSION   DATLBZ010000280 DATLBZ010000000
VERSION     DATLBZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19163
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8350071"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /gene="pyk"
                     /locus_tag="VMZ53_14750"
     CDS             complement(<1..1820)
                     /gene="pyk"
                     /locus_tag="VMZ53_14750"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HUS29760.1"
                     /translation="MPRAKIVCTLGPASASPERIGELIDAGMNVARLNFSHGTHEDHA
                     KMLAVVRAEADRKGRAVAVLLDLQGPKIRVGKFASPAGVELKPGAQFTITTDTSVIGD
                     DKRVSTVYSMLPHDVKPGDQILLDDGYLSLTVMEVSDKEVKTVVVTGGVLKNNKGINL
                     PGVEVSAPALSEKDRTDIGFAMRLGVDYVALSFVRRPEDVLEAKRLLTADQVSIPVIA
                     KIEKPQALERLVEIIDVTDGIMVARGDLGVEMGPEKVPLWQKRIIEETNKRGKIVITA
                     TQMLESMITQPRPTRAEASDVANAVLDASDALMLSGETASGVNPVEAVRTMARIIEEI
                     EKSEYYRANTEVPVINLVNSTNAIAHAAMTAARSMNLKTIVCVSDSGGAARLVSEYRP
                     EDVVEAKRLLTVDEVCIPVIAKIEKPQALDRLGDIIDVSDGIMVARGDLGVELGPEKV
                     PLWQKRIIEETNKRGKIVITATQMLESMITQPRPTRAEASDVANAVLDATDALMLSGE
                     TASGLYPVEAVKTMARIIEEIERSEYYRANTSAPEINLVNTTNAIAHAAMTAARSMKL
                     KTIVVLSHSGGAARLVSEYRPEANIICMTTNEVTFRRLALV"
     gene            1888..3777
                     /locus_tag="VMZ53_14755"
     CDS             1888..3777
                     /locus_tag="VMZ53_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HUS29761.1"
                     /translation="MRGAVHAGVPTVLCAMLCASCTVDRPQPKLEGQDIRLTVIHTSD
                     IHSRLFPYNFVPNTFDQDYGLLPANAPFGGVARISTLVKRIRRTTGRSLWLDSGDAFQ
                     GAPVFNMFKGEAEMRSLSLAGMEGEVIGNHEFDLGAKNLYEKIDSWSQFPHLAANYAW
                     DDAPGGNRSLRDVVEPYHIYDVDGLRIGVIGMGNEDTLSSIFEGGNSLGFRPVDNVLA
                     LEAYTRLIRPMVDLVVVVSHVGLDADEGLTATDVEDQNVTVPGVDLILGGHLHIVTNP
                     PKLLPNDDGSEYCKTHDCNTLLVHSGAFAKYVGRLDLVVHVGTDNADPEKRSRIVSFA
                     YKNLPVDSTIPDDPDIANLMWPYSVKLNQEIDLNGVFAYVGPEGGAKILRNDQGGGDS
                     QLGNLVARAMQVQQGVDAEFAITNSLGIRADFERGPLTNEQMYNVFPFENAITVMYLS
                     GQEVQDTLDFVARKSASRGCRTQAQVSGIAFDMVCKGDCGSIDPITGTKATACAKNIS
                     IGEHCRGTNPDGPIDPAKCAPLSLTGLYRVAVNDYIAAGGSGFIVLKRNTSQQNTGIS
                     LRDALTVYLNGQPASCNGKTTAEIPDETDTNTPQRSIEERWGKVSCLDGKIEAHDGRI
                     RPTFE"
     gene            3798..4970
                     /locus_tag="VMZ53_14760"
     CDS             3798..4970
                     /locus_tag="VMZ53_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29762.1"
                     /translation="MKYTLALLTIAGCVETRDPLMGTQSLRVELKSPTGSEASRLDPN
                     AAAPALVDVTALGPDGQPDTAYNNSVQVYVNYLGTLTPYLGNPPLATIAMSAGHAANQ
                     SVSLPRVFGPATLWLDDGTSQSPTFASGASDTIWYRDPYISDIQKPANPAALDALEKS
                     PLEQKQLRVEESEFGTATGRLVVTSVFAQGYTVSDVSCASGGAPPCTTMPYHHAMVFS
                     FSAARDEHGCIIHEGQVIDGFAGGVSEFNGLTEIGFPQSFDSRTDADCGAQDINPARI
                     PAPVALDSTWFANPIKFEENEAGLIAITNAKVCNLDTDYTTYKQWKIDPAGVGGDCKG
                     NKNVINVISTGVVPTDPMTLVGKTLPRIVGTLRPVNIGSFNVWIVYPRSAADLTLQ"
     gene            5010..6707
                     /locus_tag="VMZ53_14765"
     CDS             5010..6707
                     /locus_tag="VMZ53_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29763.1"
                     /translation="MGALCAPFAAFAQPGADQPPAPEQPATVDDAHVKELVDKEVARI
                     LNERAAQEAAQRAQAEQATKDAGATAKDDGPSDLKGSSGFMDTRLAFTITNENVLVKP
                     GETIPSVPGWRFGTPNSLGVLFFDNYDTRFSGFETLTHAVAYRTYHKDHFDAEGALVI
                     RINEISEKTLDLSDAGTYITLSNWKDPTHKDPTRISLTAFPVSSDRMRLGYSYRLSWG
                     GSPEYRRVSSRQATPGIKLQYDAKNAYAFVGAKTAVVLDRRTAEEQAEHAFLGGVGVD
                     PTDLVRIELNGGFFDRGNNELVDVNDQPVQLFGASGQIALHKGMPVQSSVDYKLYKNN
                     NERISGLFNPVKYKEGLSWLAMAEFTALGQTLKDPEKTGATVTQKGYAGDINVRVMLD
                     RIRIRFDASYRTLAFILHSQPSLPTYEDFPDEYKVSPDYFAAIGFDRNWNDFLTLGII
                     AGIDKPATLTSSKGIPGGTTGVAGQSTAVIRNNNIDTLITILPVGQKAVPQFALKTSA
                     KLDFGRIYTALVEVFYSYDGNVTRYRRVEEGNPESPFAFEFGNFNQLGINATLQARF"
     gene            complement(6709..9123)
                     /locus_tag="VMZ53_14770"
     CDS             complement(6709..9123)
                     /locus_tag="VMZ53_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HUS29764.1"
                     /translation="MKRWLAFALLTGCVSSQSVLCDEALCPEHTTCASIVYPSGEAKL
                     YCATAEEVASCAGIADGKACSGALQGTCHSGVCLENRCGNELQDVDEACDDGNTTDGP
                     LGEGDGCAADCRSNETCGNLVVDPITFEECDLADAREHDGCTSACRAETLTSTNLHSL
                     GARNAFGLAYDRERHRVVLFGGSLAGTSQNETWEWDGRRWQRVHSPISPPPRALTAVA
                     YDTDHHELVLFGGLGFFDTWTRDAFGWHLRAPSDVPHLHAAELVFDAAHHRVVVFGPD
                     PDDQKIARCATWDGTDWTEIPMPAGFIDHAASTMAYDEKRGVVVMFGGSVQLTYVNDV
                     WELDGTQWTQKTFSGPAPSPRGFAAMTYDPLAQQLLLVGGNSDSGPLGDAWLWNGTSW
                     QSTAAPTSAVTIGFAAHEMVTDSSRNVIVLVGPNGDTYERRGSTWKHIVTTSGMPGPR
                     TYPAAALDPLTREVVMSGGNNQLVGVVTDTWVWNGSWVREQSAPTGRYASAMTYDPIR
                     RELVRVGGLGTGVVDETFVRTHDTTTDVVTWTKRTPAMTPGPRRNHVLAWDGRRGRVL
                     LFGGFNGTTTVNETWSWDGTTWTHDFPTTSPSPRHSAAMAYDPIRDEVVLFGGIDAGG
                     KELADTWRWDGTTWTQVKNPSHPSARNGASLAWDPARQRLLLFGGTALLSGIFNDAWE
                     WDGTTWTMLPTTTAIPTQGHVGVSSPDGGGVLQVAGISYFDQAQELSVLRWEDVEVRD
                     DACTLTDFDGDGLAGCADPDCWPICTPLCPPGVATCAGPSCGDAVCDALREACDSCPA
                     DCGVCL"
     gene            complement(9127..9624)
                     /locus_tag="VMZ53_14775"
     CDS             complement(9127..9624)
                     /locus_tag="VMZ53_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29765.1"
                     /translation="MRAAVALLLTMTMTLAGCFPHDPHKRTIAKLAEGGALVGGIAIS
                     AFANTGADCDNNANMPGQDPSNCRSTAKWLSTLGVTLILAGLLGFVATVSTAEDEDKT
                     TATTTTVPDGAGSGSGSATTTTTTTTTTPPPTQTTPPPTDTPPPPPAGTGEGSGSATP
                     TPPTP"
     gene            complement(9648..10373)
                     /locus_tag="VMZ53_14780"
     CDS             complement(9648..10373)
                     /locus_tag="VMZ53_14780"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HUS29766.1"
                     /translation="MRLLDCLVLANCVLLGCATAASGETRVEPDQAPLVVELFTSQGC
                     SSCPPADALLNKLAHDGKLAGRPLAPLAFHVDYWDDLGWADPYALPAWSERQRQYARA
                     LGDDRVYTPELVVGGRAGMVGSDSTRAHKAIAAAPTQQKLTATASWSAKTVTIEATAP
                     ADADVLVAIYQDGTKTKVPRGENAGETLAADRVVRRLERVAPAGKTGKLTLPLDERWG
                     AIGAIAFAQRADRAIVGSALLSR"
     gene            complement(10403..11140)
                     /locus_tag="VMZ53_14785"
     CDS             complement(10403..11140)
                     /locus_tag="VMZ53_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HUS29767.1"
                     /translation="MLRLSIALVLVSGCRIAFDPLASDAAPQDTSTDVADRVIPSGPK
                     VWLRMDTDPNGGIVDSGAAHAVRCTNGPCPTLAQGRYDNGFAFNGQQVVVDYRADLDS
                     SAGFSAAIWIELRSVSTTSVTAAWTKTFNTASGFDTFALTIQTTTGQSRFDGESPAGV
                     PLTQYGPVVPTGEWHHLALTYDGTTRRDYFDGAQVMTSNVQIGVGTLPMELGFARNAY
                     YIDAVLDDAVYYTRALTQAEVTQLATP"
     gene            complement(11158..12036)
                     /locus_tag="VMZ53_14790"
     CDS             complement(11158..12036)
                     /locus_tag="VMZ53_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUS29768.1"
                     /translation="MKQLRKEVAETSRFLHQQGWCANHDGNVTARDGTRFIATPTATS
                     KRLVQERDLIEVDAKGQVHGAGKVFGEIGLHLVIYERRPDIGAVVHAHPPYATAISAS
                     RGNPIERPFIAEAFVSLGPVIPKLPYSQPGDSAKQALAPWCELVDAVILGNHGVIAWG
                     KDCETALLRLELVEHLAKIAVAANQLGGIDPLPESAYQPLLAARAKANIGRAADRAVD
                     TAQQLLGRSFTPSGAASPSSAPSSSPSSTSAKKPVVACAPAPHSETPTVPPSGGKPHD
                     IAQLVREEIVRALRGS"
     gene            complement(12033..12305)
                     /locus_tag="VMZ53_14795"
     CDS             complement(12033..12305)
                     /locus_tag="VMZ53_14795"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HUS29769.1"
                     /translation="MRQCRVVGPMWAAVKHPAFAGRPLFVVQPLDETGKDTGASFVAI
                     DTVQAGIGDKVIVLTEGNGVRQILKMGDTVPIRSMIVGIVDHVEMA"
     gene            complement(12305..12577)
                     /locus_tag="VMZ53_14800"
     CDS             complement(12305..12577)
                     /locus_tag="VMZ53_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HUS29770.1"
                     /translation="MYLGIVTGTVVAERKAAGLEGKKILLVQPVDEHKKAVGDVQAAI
                     DTVQAGVGDFVYLVGSREAALACDPWFVPVDAAIVGIVDGLDVERR"
     gene            12619..14409
                     /locus_tag="VMZ53_14805"
     CDS             12619..14409
                     /locus_tag="VMZ53_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29771.1"
                     /translation="MERDDDPPTLKSMGSAPDTLVKTTPHPKQSVGDVEDVTKSLDGY
                     AFGAVIGRGGVGEVVAAHDLRMGRDVAIKRLRTTSPSADEVNRFLREARIQARLDHPA
                     IPPVHELGAGPDGKPFFTMKKLVGQTLSELLAAPEPNRPRLLRAFVEVCQALDFAHAR
                     GVVHRDLKPGNIMLGDYGDVYVLDWGLARVVGEAITEVVTDDIDSLDLKSGDILGTPG
                     YMAPEQLQRVGDTGRPADIYALGSILFELLAGEPLHPRGPTAIASTVGNEAVTSAAKR
                     RPDRAVPPELDAICVAMLTVDPTTRPTARRVADRVQAYLDGDRDVARRKTMAVDLVWS
                     ARAQMNEGNRADAMRAAGRALALDPESTGAAELVTALMLEPPKEPPAELKMALRDAED
                     TGVRRHARTAILAYLAIASFLPIAMWNGIRKWDVVLAVFVVAIVMAAGAFHIMRRGQP
                     SNAFMMLYAFGNALLLGVLTRLAGPFTFVPALSCVVIMSVMAYPLFTVRSWVLIVMIA
                     GGFMVTIALELNGMLPMTWEIQEGVLISHAGALSLRGDPTLALLISSTVATIVIAGIH
                     AARIYRAGRDARLQLVTQAWHLRQLLPKTA"
     gene            complement(14406..15224)
                     /locus_tag="VMZ53_14810"
     CDS             complement(14406..15224)
                     /locus_tag="VMZ53_14810"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HUS29772.1"
                     /translation="MEALVRIVRRIIGGAPIVMAISCTGPRVDKEPPKPAVTVAPDVT
                     DRVEDNTPKQLGLFDITFYYVVTEDDVVPKAAPANDNTSEVTLAAGTQPELVTLYDYK
                     SCEPIAEVTPEFASMARLQGTGKLRDGRLVNIASRCDCETKRCFQVLSEKIQWGTAGT
                     GKPLQPFRTVAVDPKVVKLGALLYVPLLEGRTMPGRAPWGGFVHDGCVVADDTGGGID
                     DYQLDLFVGKKAYYQGMKSAHGGSHAWARHVPVYDGSKICERKGRRVGKKQASI"
     gene            complement(15321..19145)
                     /locus_tag="VMZ53_14815"
     CDS             complement(15321..19145)
                     /locus_tag="VMZ53_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29773.1"
                     /translation="MRVAQEHKAWDRLADLYEGMAESADTAVAAADLLMEVAQIRYEQ
                     KRPREAEAQLRRILGMLPNDATARSRLEELYRNEGRWVELAASLEERSDPRLGTAAPE
                     SERPQLLRELAAIYVDKLHRTHDALDALERLRNLAPADIAILNQVASLYADVGRWSKV
                     IETLSRITEIAEGTEDMRQALHAIARIYVKELEQPGKAIDAYVQTVTTWPDDIDAWGA
                     LDELYQSAQRWAELADVLRRRAALAREPAERSALLARRAHVLIDHLDSPEEAAAALKH
                     ARTVAPDDPALADQMVAALSKAGRSREAAAILEGRIEQLAEQLKNAPPEPVATAPTKP
                     PPGAKRPAPGPKAQGTDKSQPTATTKGSSRGDLAVLYIRLAQLRNAEEDKPGARAAID
                     QALALVPEHPTALSVLAELSSPDEDPRAFADAKLREADSARDEDNKIAALMAAGQVLQ
                     QRVGDLAAAQSAYERVLALRPYHADATWALAGLSEQGGNAESAQHILEKKLEDESLTP
                     PEKARILTQLAALSRAAGVEPAAERRLLEALGTVPDHIPAIVALADFYADALRWNDLE
                     AFLREILDGTTLTGAPGALVADLHRRLATAHEKLGRDDDAYQTLTAADRLHRGHLLIK
                     LALGENRYKARRWRESALHLSPLATHEDAAKYPSEVAQGLYHAALAEIRSLRPEKAPA
                     LYERALELKPNYGPALQALAEIAMEQGDHRKAADLLTRQATATEDPGERLKLFEALGD
                     TSLMMLKDEERARTCYAAAVQSAQPLEAKHVPLLEKLLDLQNKHGDLAGSARTAELLA
                     AFGATPADRAARHLSAAHDYLAAGDKIRARAAAERAVEHDPYDVDAVDLASTLALDQT
                     DFDAAVGMLTRLLTAKDDRFTATQAAHRAMLSYRLGHARTQRGDVRQAIPALERAVQL
                     APESDGATLARRSLVELSKQSDDPNRKEQAAAHLAAITHATGALADLVAWGDEMRRQN
                     RADAARATLELAIACGHTADVHQSAYLQINKAYQMRDDESYKAVVDDPSMLVSEDHPL
                     AAIASTLAEAAALIWPDLEETLSRAGCAGAKRIPATSKAAAVGMFPRLTTALGTGAVM
                     LYFADTQADVTVVAAGTPVIVLGRRLTSEASPPPLEETRALLARAVQLTKPEHLAFAG
                     LPPRDATRLLTSVVRLFGPPALREVVSAFVDEDVQRAHDEAVKGALPVKLRTRLEGLL
                     ATMPAGALDNARYLMVCERNADRAALLVGGAPQTIVACAKTRGDGVEHLIRAIGHPSW
                     LATRQKLGVGVR"
BASE COUNT         3145 a         6369 c         6325 g         3324 t
ORIGIN      
        1 accagcgcga ggcggcggaa cgtgacctcg ttcgtcgtca tgcagatgat gttcgcctcg
       61 gggcgatact cgctgacgag ccgcgcggcg ccgcccgagt gggagagcac gacgatcgtc
      121 ttgagcttca tcgaccgggc cgcggtcatc gcggcatgcg cgatcgcgtt cgtcgtgttg
      181 acgaggttga tctcgggagc cgacgtgttc gcgcggtagt actcgctgcg ctcgatctcc
      241 tcgatgatcc gcgccatcgt cttgaccgcc tcgactgggt acagccccga cgcggtctcg
      301 cccgacagca tcagcgcgtc ggtcgcgtcg agcaccgcgt tcgcgacgtc cgacgcctcg
      361 gcgcgcgtcg ggcgcggctg cgtgatcatc gactcgagca tctgcgtcgc ggtgatgacg
      421 atcttgccgc gcttgttggt ctcctcgatg atccgcttct gccacagcgg gaccttctcg
      481 ggtccgagct cgacgccgag atcgccgcgc gcgaccatga tgccgtccga gacgtcgatg
      541 atgtcgccga gccggtcgag cgcctgcggc ttctcgatct tcgcgatcac cgggatgcag
      601 acctcgtcga ccgtcagcag gcgcttggcc tcgaccacgt cctcgggccg gtactcgctg
      661 acgaggcgcg cggcgccgcc cgagtccgag acgcacacga tcgtcttcag gttcatcgac
      721 cgcgccgccg tcatcgcggc gtgcgcgatc gcgttcgtcg agttgacgag gttgatcacc
      781 gggacctcgg tgttcgcgcg gtagtactcg ctcttctcga tctcttcgat gatgcgcgcc
      841 atcgtgcgca ccgcttcgac cgggttcacg ccgctcgcgg tctcgcccga gagcatgagc
      901 gcgtcgctcg cgtcgagcac cgcgttcgcg acgtcacttg cttccgcgcg cgtcgggcgc
      961 ggttgcgtga tcatcgactc gagcatctgc gtcgcggtga tcacgatctt tccgcgcttg
     1021 ttcgtctctt cgatgatgcg cttttgccag agcggaactt tttccggccc catctcgacg
     1081 cccagatcgc cgcgcgcgac catgatgccg tccgtgacgt cgatgatttc gacgagccgc
     1141 tcgagcgcct gcggcttttc gatcttggcg atcaccggga tcgagacctg atcggccgtc
     1201 agcaggcgct tcgcttcgag gacatcttcg ggccgccgca cgaacgacag cgcgacgtaa
     1261 tcgacaccga ggcgcatcgc gaaaccgatg tccgtgcggt ccttctctga cagcgcgggg
     1321 gcgctgacct cgacgccggg caggttgatg cccttgttgt tcttcagcac gccgccggtg
     1381 acgacgaccg tcttgacctc tttgtccgag acttccatca cggtcagtga gagatatccg
     1441 tcgtcgagca ggatctgatc gcccggcttc acgtcgtgcg gcagcatcga gtaaacggtc
     1501 gagacgcgct tgtcgtcgcc gataacactc gtgtccgtcg tgatcgtgaa ctgcgctccg
     1561 ggcttgagct cgacgcccgc cggagaagcg aactttccga cgcggatttt cggaccttgc
     1621 aggtcgagca gaaccgcaac cgcgcggcct ttccggtcgg cctctgcgcg gacgaccgcg
     1681 agcatcttgg cgtgatcctc gtgcgtaccg tgcgaaaaat tcagacgcgc gacgttcatg
     1741 cccgcgtcga ttagctcgcc gattcgctcg ggcgaggcgg aggcagggcc gagggtacag
     1801 acgatcttcg cccggggcat cggaggcgca gtagaccatc gaatcctatt gctgaaaacc
     1861 ggccgccccg ggtattaccg cgggccaatg cgtggcgctg tacacgcggg tgttccgacg
     1921 gtgctctgcg ccatgctgtg cgcgagctgc accgtcgatc gtccgcagcc gaaactcgaa
     1981 ggtcaggaca ttcgcctgac cgtcatccat acgtcggaca tccactcgcg gctgttcccg
     2041 tacaacttcg tccccaacac gttcgatcag gactacggac tgctccccgc gaacgcaccg
     2101 ttcggcggcg tggctcgcat ctcgacgctc gtgaagcgca tccgtcgcac gacgggccgc
     2161 tcgctgtggc tggactccgg cgacgcgttc caaggcgcgc ccgtcttcaa catgttcaag
     2221 ggcgaagccg aaatgcgctc gctctcgctc gccggtatgg agggcgaggt catcggcaat
     2281 cacgagttcg atctcggcgc gaagaacctc tacgagaaga tcgactcgtg gtcgcaattc
     2341 ccgcacctcg ctgcgaacta cgcgtgggac gacgcgccgg gcggcaaccg ctcgctgcgt
     2401 gacgtcgtcg agccctatca catctacgat gtcgacggcc tgcgcatcgg cgtcatcggc
     2461 atgggcaacg aagacacgct gtcgtcgatt ttcgaaggcg gcaactccct cggcttccgc
     2521 cccgtcgaca acgtcctcgc gctcgaggcg tacacgcgcc tgattcggcc gatggtcgat
     2581 ctcgttgtcg tcgtcagcca cgtcggcctc gatgcggacg aaggcctcac cgcgacggac
     2641 gtcgaagatc agaacgtcac cgtgcccggc gtcgatctca ttctcggcgg tcacctgcac
     2701 atcgtcacga atcccccgaa gctcctgccg aacgacgacg gctccgagta ctgcaagacc
     2761 cacgattgca acacgctgct cgttcactcg ggcgcgttcg cgaaatacgt cggccgcctc
     2821 gacctcgtcg ttcacgtcgg caccgacaac gccgaccccg agaagcgcag ccgcatcgtg
     2881 tcgttcgcgt acaagaacct gcccgtcgac tcgacgattc ccgacgatcc ggacatcgcg
     2941 aatctgatgt ggccgtactc ggtgaagcta aaccaagaga tcgatctcaa cggcgtgttc
     3001 gcctacgtcg gccccgaagg cggcgcgaag attttgcgca acgaccaagg cggcggcgac
     3061 agccagctcg gcaacctcgt cgcgcgcgcg atgcaggtgc agcagggcgt cgatgcagaa
     3121 ttcgcgatca cgaactcgct cggtattcgc gcggacttcg agcgcggccc gctgacgaac
     3181 gagcagatgt acaacgtgtt cccgttcgag aacgcgatca ccgtgatgta cctatcgggt
     3241 caggaagtgc aggacacgct cgatttcgtc gcgcgcaaga gcgcatcgcg cggctgccgc
     3301 acgcaagcgc aggtcagcgg catcgcattc gacatggtgt gcaaaggcga ttgcggcagc
     3361 atcgatccga tcaccggaac gaaggcgacc gcatgcgcga agaacatctc gattggcgag
     3421 cactgccggg gcacgaaccc cgacgggccg atcgatccgg ccaagtgcgc gccgctctcg
     3481 ctgaccggcc tctatcgcgt cgccgtcaac gattacatcg cggccggtgg ctcgggcttc
     3541 atcgtgctga agcgcaacac gtcgcagcag aacactggca tctcgctgcg cgacgcactc
     3601 accgtctatc tcaacggcca gccggcttcg tgcaacggca agacgaccgc cgagatcccc
     3661 gacgagaccg acacgaacac gccgcagcgc tcgatcgaag agcgatgggg caaggtgtcg
     3721 tgcctcgacg gcaagatcga agcgcacgat ggccgcatcc gcccgacgtt cgaatgagcc
     3781 cgcgaatgag agcctccatg aaatacaccc tcgcgctgct cacgatcgcc ggttgcgtcg
     3841 agacgcgcga tccgctcatg ggcacgcagt cgctgcgcgt cgagctgaag tcgccgacag
     3901 gaagtgaagc gagccgcctc gatccgaatg cggcagcgcc cgcgctcgtc gacgtcaccg
     3961 cgctcggccc cgacggccag cccgataccg cgtacaacaa ctcggtccag gtctacgtca
     4021 actacctcgg cacgctcacg ccgtatctcg gcaacccgcc gctcgcgacg atcgcgatga
     4081 gcgctggcca tgccgcgaat caatcggtct cgctgccgcg cgtgttcggt ccggcgacgt
     4141 tgtggctcga cgacggtacg tcgcaatcgc cgacgttcgc gtcgggcgca tcggacacga
     4201 tctggtatcg cgatccgtac atcagcgaca tccagaagcc cgcgaacccg gcggcgctcg
     4261 atgcgctcga gaaatcgccg ctcgagcaga agcagctgcg cgtcgaggaa tccgaattcg
     4321 gcacagccac cgggcgcctc gtcgtgacga gcgtgttcgc gcagggctac acggtctcgg
     4381 acgtgtcatg cgccagcggc ggcgcgcctc cgtgcacgac gatgccgtac caccacgcga
     4441 tggtgttctc gttctcggcc gcccgcgacg agcacggctg catcattcac gaaggccaag
     4501 tcatcgacgg gttcgccggc ggcgtgtcgg agttcaacgg cctcaccgag atcgggttcc
     4561 cgcaatcgtt cgattcgcgc accgacgccg actgcggcgc gcaggacatc aacccagctc
     4621 gtatcccggc gccggtcgcg ctcgactcga cgtggtttgc aaacccgatc aagttcgagg
     4681 agaacgaggc gggcctcatc gcgatcacga acgcgaaggt ctgcaacctc gacaccgact
     4741 acacgacgta caagcagtgg aagatcgatc ccgccggggt cggcggcgac tgcaagggca
     4801 acaagaacgt catcaacgtg atctccaccg gcgtcgtgcc gaccgatccg atgacgctcg
     4861 tgggcaagac actgccgcgc attgtcggta ccctccgccc ggtcaatatc ggcagcttca
     4921 acgtctggat cgtttatccg cgctcggctg cggatctgac tctgcaatag ggtgacttca
     4981 tgggtgctcg tttagctttc gctctcgtta tgggcgcgct ctgcgctccg tttgccgcct
     5041 tcgcacaacc cggcgccgat cagcctcctg cgccggagca gccagcgacg gtcgacgacg
     5101 cgcacgtcaa agagctcgtc gacaaagaag tcgctcgcat cttgaacgaa cgcgcagcgc
     5161 aagaagcggc gcagcgggcg caggcggagc aagccacgaa agatgccggc gcgacggcga
     5221 aagacgacgg cccatcggac ctcaagggtt cgagcggctt catggacacg cgtctcgcgt
     5281 tcacgattac gaacgagaac gtgctcgtca aaccgggcga gaccattccg agcgttccag
     5341 gttggcgctt cggcacgccg aactcgctcg gcgtcctgtt cttcgacaac tacgacacgc
     5401 ggttctcggg cttcgagacg ctgacgcacg ccgtcgccta ccgcacgtat cacaaggatc
     5461 acttcgacgc cgagggcgcg ctcgtcatcc gcatcaacga gatcagcgag aagacgctcg
     5521 atctctccga cgccggcacg tacatcacgc tctcgaactg gaaggatccg acgcacaaag
     5581 atccaacgcg catctcgctc accgcgttcc cggtgtcctc cgaccgcatg cgcctcggct
     5641 actcgtatcg cctctcgtgg ggtggcagcc ccgagtaccg ccgcgtgagc tcgcgccaag
     5701 cgacgccggg catcaagctc cagtacgacg cgaagaacgc gtacgcgttt gtcggcgcga
     5761 agaccgccgt cgtcctcgat cgccgaaccg ccgaagagca agcggagcac gcgttcctcg
     5821 gcggcgtcgg tgtcgatccg acggacctcg ttcgcatcga gctcaacggc ggcttcttcg
     5881 atcgcggcaa taacgagctc gtcgacgtca atgatcaacc cgtgcagctg ttcggcgcgt
     5941 cgggacaaat cgcgttgcat aagggcatgc ccgtgcagtc gtcggtcgac tacaagctct
     6001 acaaaaacaa caacgagcgc atcagcggcc tgttcaatcc ggtgaagtac aaagaaggcc
     6061 tgtcgtggct cgcgatggcc gaattcaccg cgctcggcca gacgctcaaa gatcccgaga
     6121 agaccggcgc gacggtcacg caaaagggct acgccggcga catcaacgtt cgcgtgatgc
     6181 tcgatcgcat tcgcatccgc ttcgacgcca gctatcgcac gctcgcgttc attctccaca
     6241 gccagccgtc actgccgacg tatgaagact tccccgacga gtacaaggtc tcgccggact
     6301 acttcgcggc gatcggcttc gatcgcaact ggaacgactt cctcacgctc ggcatcatcg
     6361 cgggcatcga caagccggct acgctgacgt cgagcaaggg cattccgggc ggcaccaccg
     6421 gcgtcgccgg ccagtcgacc gcggtcatcc gcaacaacaa catcgacacg ctgatcacga
     6481 tccttcccgt cggccagaag gcggttccgc agttcgcgct caagacgtct gcgaagctcg
     6541 acttcggccg catctacacc gcgctcgtcg aggtcttcta cagctacgac ggcaacgtca
     6601 ctcgctaccg ccgcgtcgaa gagggcaacc cagagtcgcc gttcgcgttc gagttcggca
     6661 acttcaatca gctcggcatc aacgcgacgc tgcaagcgcg cttctgatct agaggcacac
     6721 gccgcagtcg gcgggacacg agtcgcaggc ttcgcggagc gcgtcgcaca cggcgtcgcc
     6781 gcagctcgga cccgcacacg tggcgacacc cggcgggcag agcggcgtgc agatcggcca
     6841 gcagtctgga tccgcgcagc ccgcgaggcc atcgccgtcg aagtccgtca gcgtgcacgc
     6901 gtcatcgcga acctcgacgt cttcccagcg gaggacgctg agctcttgcg cctgatcgaa
     6961 gtacgagatg cctgcgacct gaagcacgcc gccgccgtct ggtgacgaca cgccgacgtg
     7021 gccttgcgtt ggaatcgccg tggtcgtcgg gagcatcgtc cacgtagtgc cgtcccattc
     7081 ccacgcatcg ttgaagatgc cgctgagcaa tgcggtgcca ccgaacagca agaggcgttg
     7141 gcgcgcagga tcccaggcga gcgaagcgcc gttgcgcgca gaagggtgcg aaggattttt
     7201 gacctgcgtc cacgtggtcc cgtcccagcg ccacgtgtcg gctagctcct taccgcccgc
     7261 gtcgatgcca ccgaaaagca cgacttcgtc gcgaatcgga tcgtaggcca tcgcggcgct
     7321 gtgtcgcggg cttggtgacg tcgtcgggaa gtcatgtgtc cacgtcgtgc cgtcccacga
     7381 ccacgtctca tttacggtcg tggtcccgtt gaagccaccg aacagcagca cgcgtccacg
     7441 ccggccgtcc catgcgagga cgtggtttct gcgaggaccg ggcgtcatcg ccggcgtgcg
     7501 cttcgtccac gtgaccacgt ccgtggtcgt gtcatgcgtg cgcacaaacg tctcgtcgac
     7561 gactccggtc ccgaggccgc cgacgcggac gagctctcga cgaatcggat cgtaggtcat
     7621 cgccgaggcg tagcgcccag tcggcgcgct ttgctcgcgc acccacgagc cgttccatac
     7681 ccacgtatcg gtgacgacac caacgagctg gttgttgccg cccgacatca cgacctcgcg
     7741 cgtaagcgga tcgagcgcgg cggctggata cgttcgagga ccgggcattc cgctcgtggt
     7801 cacgatgtgc ttccacgtcg agccgcgccg ttcgtacgtg tcaccgttgg ggcccaccag
     7861 cacgatcacg ttgcgcgacg agtcggtcac catctcgtgg gcggcaaacc cgatcgtaac
     7921 ggcgctcgtc ggcgccgccg tcgactgcca actcgttccg ttccagagcc acgcatctcc
     7981 gaggggccca ctgtccgaat tcccgccgac gaggagcagc tgctgcgcga gcggatcgta
     8041 cgtcatggct gcgaatcctc tcggcgatgg cgcggggccc gagaacgtct tctgcgtcca
     8101 ctgcgtgccg tcgagctccc agacgtcgtt gacgtacgtg agctgcaccg agccaccaaa
     8161 catcacgaca acgccgcgtt tctcgtcgta cgccatcgtg ctcgccgcat ggtcgatgaa
     8221 gccggccggc atcgggatct cggtccagtc ggtgccgtcc cacgtcgcgc agcgcgcgat
     8281 tttttggtcg tcaggatctg ggccgaagac gaccacgcgg tggtgcgcgg cgtcgaacac
     8341 gagctctgcc gcatgcaggt gcggcacgtc ggacggtgcg cggagatgcc agccaaatgc
     8401 atcgcgcgtc cacgtgtcga agaagccaag cccaccgaag aggacgagct cgtgatgatc
     8461 ggtgtcgtac gccacggctg tgagtgcgcg cggcggtggc gagatcgggg aatgcacgcg
     8521 ctgccagcgc cgaccgtccc actcccacgt ctcgttctgc gatgtgccgg cgagcgagcc
     8581 accgaacaag accacgcggt ggcgttcgcg gtcgtacgcg agtccaaacg cgttgcgtgc
     8641 tccgagcgag tggaggttcg tcgacgtcag cgtttcagcg cggcacgcgc tcgtacaacc
     8701 gtcgtgctcg cgcgcatcgg cgaggtcgca ctcctcgaac gtgatcggat cgacgacgag
     8761 gttgccgcac gtctcgtttg atcggcagtc ggccgcacag ccgtcgcctt cgccgagcgg
     8821 gccgtccgtc gtgttgccgt cgtcgcatgc ctcgtcgaca tcctgcagct cgttgccaca
     8881 gcgattctcg aggcacacgc cgctgtgaca cgtgccctgc aacgcgccgc tgcatgcttt
     8941 gccgtccgcg atacccgcgc agctcgcaac ttcctcggct gtcgcgcagt acagcttcgc
     9001 ttcgccgctc ggatagacga tcgacgcgca ggttgtatgt tctgggcaga gcgcttcgtc
     9061 gcagaggacg gattgcgacg acacgcatcc ggtcaggagc gcgaatgcga gccagcgctt
     9121 cacgtgttac ggcgtcggcg gcgtcggagt ggccgagccg gagccttcgc cggtgcccgc
     9181 aggcggtggc ggcggcgtat ccgtcggcgg tggcgtcgtc tgcgtcggag gcggcgtggt
     9241 ggtcgtggtc gtggtcgtgg tcgtcgccga gccagagccc gatccagcgc cgtcgggaac
     9301 cgtcgtcgtc gtcgcggtcg ttttgtcctc gtcctcggcg gtcgacaccg tcgcgacaaa
     9361 gccgagcaag ccggcgagga tgagcgtcac gccgagtgtc gagagccact tcgccgtcga
     9421 gcgacagttc gacggatctt ggcccggcat gttcgcgttg ttgtcgcagt cggcgccggt
     9481 gtttgcgaac gcggagatgg cgataccgcc gacgagcgcg ccgccctcgg cgagcttcgc
     9541 gatggtccgc ttgtgcggat cgtgcgggaa gcagcccgcg agcgtcatcg tcatcgtgag
     9601 aaggagagca accgctgcac gcatgatgag cggttttacc agaggactca gcgcgacagg
     9661 agcgcagagc ccacaatggc gcggtcggct cgttgggcaa aggcaatcgc cccgatggct
     9721 ccccaccgct cgtcgagcgg gagtgtgagc ttcccggtct ttcccgcggg tgcgacacgc
     9781 tcgaggcgtc gcaccacccg gtccgcggcg agggtctccc ccgcgttttc tccgcgcggc
     9841 accttcgtct tcgtgccgtc ttgatagatc gcgacgagca cgtcggcgtc ggcgggtgcg
     9901 gtggcttcga tggtgacggt cttcgcggac cagctcgcgg ttgcggtgag cttctgttgc
     9961 gtgggcgcgg cggcgatcgc tttgtgcgcg cgcgtcgaat ccgagccgac cattcctgcg
    10021 cggccaccga cgacgagctc gggcgtgtac acgcgatcgt cgccgagcgc gcgtgcgtat
    10081 tgacgctggc gctctgacca cgcgggcagg gcatacggat cggcccagcc gaggtcgtcc
    10141 cagtagtcga catgaaatgc gagcggcgcg agaggacgac cggcgagctt gccgtcgtgc
    10201 gcgagcttgt tcagcagcgc gtcggcgggc gggcacgagc tgcagccttg cgacgtgaac
    10261 agctcgacga cgagcggtgc ttgatccggc tcgacgcgcg tttcgccaga tgccgcggtg
    10321 gcgcagccga ggagcacgca gttcgcgaga acgaggcagt cgaggagccg catggtgtgc
    10381 ttacgccgcc gagggtagcc ggttacgggg tcgcgagctg cgtgacttcg gcctgggtga
    10441 gtgcgcgggt gtagtagacg gcgtcgtcga gaacggcatc gatgtagtac gcattgcgcg
    10501 caaacccgag ctccatcggc agcgtcccga cgccgatctg gacgttgctc gtcatcacct
    10561 gcgcgccgtc gaagtagtcg cggcgggtcg tgccgtcgta cgtgagcgcg aggtggtgcc
    10621 attcgcccgt cgggacgacc gggccgtact gcgtgagcgg gacgcccgct ggcgattcgc
    10681 cgtcgaagcg gctctggccg gtcgtcgttt gaatcgtgag cgcgaacgtg tcgaagccgc
    10741 tcgcggtgtt gaacgtcttg gtccacgcag ccgtgacgct cgtcgtggag acggaccgca
    10801 gctcgatcca gatcgcagcg ctgaagcccg cgctcgagtc caaatcggct cggtagtcga
    10861 cgacaacctg ctggccgttg aacgcgaagc cattgtcgta gcgtccctgc gccagcgtcg
    10921 gacagggtcc gttggtgcaa cgcaccgcgt gtgcagctcc gctgtcgacg atgccgccgt
    10981 tcgggtcggt atccattcgc agccagacct tcgggccgga cgggatgact cggtcggcga
    11041 catccgtcga tgtgtcctgc ggcgcggcat cggacgcgag aggatcaaat gcgatcctgc
    11101 acccgctgac gagcacgagg gcaatcgaga gccgcagcac gcgctcgatg gtacagccta
    11161 cgagccgcgg agcgcgcgca cgatctcttc gcgtacgagt tgagcgatat cgtgcggttt
    11221 gccgccgctg ggcgggaccg tcggagtctc ggagtgcggt gcgggcgcgc atgcgacgac
    11281 cggctttttc gcggacgtcg acgacggcga cgacgaggga gccgatgacg gagacgctgc
    11341 gcccgacggt gtgaacgagc gcccgagcag ttgctgcgcg gtgtcgacag cgcgatcggc
    11401 agcgcggccg atgttcgctt tcgcgcgcgc ggcgagcagc ggctggtacg cggactcggg
    11461 cagcggatcg atcccgccga gctggttcgc agcgaccgcg atcttcgcga gatgctcgac
    11521 cagctcgaga cgaaggagcg cggtctcgca gtccttgccc cacgcgatca caccgtggtt
    11581 gccgagaatg acggcatcga cgagctcgca ccacggcgcg agtgcctgct ttgcggaatc
    11641 accgggctgc gagtacggca gcttcgggat caccgggccg agcgagacga acgcttccgc
    11701 gatgaacggg cgctcgatcg gattgccgcg gctcgcgctg atcgcggtcg cgtacggcgg
    11761 atgcgcgtgg acgactgcgc cgatatcggg acgacgttcg tagatgacga ggtgcaggcc
    11821 gatctcgccg aagaccttgc cggcgccgtg cacctggccc ttcgcatcga cctcgatcaa
    11881 gtcgcgctct tgcacgaggc gcttggacgt cgccgtcggc gttgcgatga agcgggtgcc
    11941 gtcgcgcgcg gtgacgttgc catcgtggtt cgcgcaccaa ccctgctgat ggaggaagcg
    12001 cgacgtctcg gcgacctctt tgcggagctg cttcacgcca tctccacgtg atcgacgatg
    12061 ccgacgatca tcgagcgaat cggaaccgtg tcgcccatct tcagaatctg gcgcacgccg
    12121 ttgccctcgg tgagcacgat gaccttgtca ccgatgccgg cttgcacggt gtcgatcgcg
    12181 acgaagctcg cgcccgtatc cttgccggtc tcgtcgagcg gctggacgac gaacagtggc
    12241 cggcctgcaa acgccggatg cttcaccgca gcccacatgg gaccgacgac gcggcactga
    12301 cgcattagcg ccgctccacg tcgaggccat cgacgatgcc gacgatcgct gcatcgaccg
    12361 ggacgaacca cggatcgcac gcgagtgccg cttcgcgcga gcccaccaga tagacgaaat
    12421 cgccgacgcc ggcctgcacg gtgtcgatcg ccgcttggac gtcgccgacg gccttcttgt
    12481 gctcgtcgac gggctgcacg agcaggatct tcttgccctc gaggccggcg gctttgcgct
    12541 cggcgaccac ggtgccggtg acgatgccca agtacatcgg ccgcgactat aaacccaact
    12601 cgggcgtacg atcgctagat ggagcgggat gatgaccctc cgacgctgaa gtcgatgggc
    12661 tcggcgccgg acacgctggt gaaaacgaca cctcacccca agcagtcagt gggcgacgtg
    12721 gaggacgtca cgaaatctct cgacggctac gccttcggcg ccgtgatcgg gcgcggcggc
    12781 gtcggcgagg tggtggcggc tcatgatctg cgaatggggc gcgacgttgc gatcaagcgg
    12841 ctgcggacga ccagcccgtc cgcggacgag gtgaaccggt tcctgcgaga agcccgcatt
    12901 caagcgcgcc tcgatcatcc cgcgatcccg ccggtgcacg agctcggagc gggaccggac
    12961 ggcaagccgt tcttcacgat gaaaaagctc gtcggccaga cgctctcgga gctgctcgcg
    13021 gcgcccgagc cgaatcgccc gcgcttgctt cgcgcattcg tcgaggtctg ccaggcgctc
    13081 gacttcgcgc acgcacgcgg tgtcgtccat cgcgatctca agcccggcaa catcatgctc
    13141 ggcgactacg gcgacgtcta cgtcctcgat tgggggctcg cacgcgtcgt cggtgaagca
    13201 atcaccgagg tcgtcaccga cgacatcgac agcctcgatc tcaagagcgg cgacatcctg
    13261 ggcacgcccg gctacatggc acccgagcag ctgcaacgcg tcggcgacac gggccggccc
    13321 gcagacatct atgcgctcgg ctcgatcttg ttcgagctcc tcgcgggcga gccgctgcat
    13381 ccgcgtgggc ccacggcgat cgcgagcacc gtcggcaacg aggccgtgac atccgcggcg
    13441 aagcgccgtc ccgatcgcgc agtgccgccg gagctcgacg cgatctgcgt cgcgatgctc
    13501 acggtggatc cgacgacgcg gccgaccgca cgccgtgttg cggatcgcgt gcaggcatat
    13561 ctcgacggcg atcgcgacgt cgcgcgccgc aagacgatgg ccgtcgatct cgtgtggagc
    13621 gcgcgtgcgc agatgaacga aggcaaccgt gccgacgcca tgcgtgcagc cggtcgcgcg
    13681 ctcgcactcg atccagaatc gaccggtgcg gcggagctcg tcacggcgct gatgctcgag
    13741 ccgccgaagg agccgcccgc cgagctgaag atggcgctgc gagacgcgga agacaccggc
    13801 gttcgccgcc acgcgcgcac cgccatcctc gcgtatctcg cgatcgcatc gttcttgccg
    13861 atcgcgatgt ggaacggcat ccgcaagtgg gacgtcgtcc tcgccgtgtt tgttgtggcc
    13921 atcgtgatgg cggcgggcgc gttccacatc atgcggcgcg gtcaaccgtc gaatgcgttc
    13981 atgatgctct acgccttcgg caacgcattg ctgctcggcg tgctgacacg tctagcagga
    14041 ccgttcacgt tcgtgccggc gctgtcgtgc gtcgtgatca tgagcgtgat ggcgtatccg
    14101 ctgtttacgg tgcgctcgtg ggtgctgatc gtgatgatcg caggcggctt catggtgacg
    14161 atcgccctcg agctaaacgg catgttgccg atgacgtggg agatccaaga aggtgttctc
    14221 atcagccacg caggcgcgct gtcgctacga ggtgatccga cgctcgcgtt gctgatcagc
    14281 tcgacggtcg cgacgatcgt gatcgccggc attcacgcgg cgaggatcta tcgcgcagga
    14341 cgcgacgcgc gactgcagct cgtcacgcaa gcgtggcatc tgcgtcagct gttgccgaag
    14401 accgcttaga tcgacgcttg cttcttgccg acgcgacggc cttttcgctc gcagatcttc
    14461 gagccgtcgt acaccggcac gtgacgcgcc cacgcatgac tgccgccgtg cgcgctcttc
    14521 atgccttggt agtaggcctt cttgccgacg aacaaatcga gctggtagtc gtcgatgccg
    14581 ccgccggtat cgtctgcgac gacgcagccg tcatggacga agccgcccca tggcgcgcgc
    14641 cccggcatcg ttcggccttc gagcagcggg acatagagca gggcgccgag cttgacgacc
    14701 ttcgggtcga cggcgaccgt gcggaacggc tggagcggct tgccggtgcc cgccgtgccc
    14761 cattggatct tctcgctgag gacctggaag cagcgcttgg tctcgcagtc gcagcgactc
    14821 gcgatgttga cgagccggcc gtcgcgcagc ttgccggtgc cttgcagtcg ggccattgac
    14881 gcgaattcgg gcgtcacttc ggcgatgggc tcgcagctct tgtagtcgta gagcgtgacg
    14941 agctcgggct gcgtgcctgc tgcgagggtg acctcggagg tgttgtcgtt cgcgggcgcg
    15001 gccttcggaa cgacgtcgtc ttcggtcacg acgtagtaga acgtaatgtc gaacaatccc
    15061 agttgcttgg gcgtgttgtc ttcgaccctg tcggtgacgt ccggcgccac ggtcacggca
    15121 ggcttcggcg gctctttgtc gacccgtggc cccgtgcaac tgatcgccat gacgatcggc
    15181 gcgccgccga taatccgtct gacgattcgt actagggctt ccaccgagga tcgatggtag
    15241 cgggcgccct ccggagtaaa gagccggtcc gcgcgaaaag aacgtgagac cggctaaatg
    15301 ccgttcactt ggtgaagcgc ttaccgaact ccgacgccga gcttctgccg cgtcgcgagc
    15361 cagctcggat ggccgatcgc ccggatcagg tgctcgacac catcgccgcg agtcttcgcg
    15421 cacgcgacga tcgtttgcgg tgcaccgccg accagtagcg cagcgcgatc cgcgttgcgt
    15481 tcgcacacca tgaggtaacg cgcgttgtcg agcgcgcccg cgggcatcgt cgccagcaaa
    15541 ccttcgagcc gcgtgcgcag cttcacgggc agcgcgccct tcaccgcttc gtcatgcgcg
    15601 cgctgcacat cctcgtcgac gaatgccgag acgacttcgc gcaacgcggg cggtccgaac
    15661 aggcgcacga ccgacgtgag caagcgcgtt gcatcgcgcg gaggcagacc tgcgaacgcg
    15721 aggtgctcgg gctttgtgag ctggacggcg cgagcgagca gtgcgcgagt ctcctcgagt
    15781 ggcgggggcg atgcttcgct cgtcaggcga cgaccgagaa cgatcaccgg ggtgccggca
    15841 gcgacgacgg tgacgtccgc ttgcgtgtcc gcgaagtaca gcatcacggc gccggttccg
    15901 agcgccgtcg tgaggcgagg gaacatgccg acggcggccg ccttgctcgt cgcagggatc
    15961 cgcttggccc ccgcgcaacc agcgcgcgag agggtctctt cgagatcggg ccagatcagc
    16021 gcggccgctt cagcgagcgt cgacgcgatc gcggcgagcg gatgatcctc gctgacgagc
    16081 atcgacggat cgtcgacgac ggccttgtac gactcgtcgt cgcgcatctg gtaggccttg
    16141 ttgatctgga gatacgcgct ctggtgaacg tctgcggtgt gaccacacgc gatcgcgagc
    16201 tcgagcgtgg cacgcgcggc atcggcgcga ttttggcggc gcatctcgtc gccccaggca
    16261 acgaggtctg caagtgcgcc cgtcgcgtgc gtgatcgcag cgaggtgagc ggcggcttgt
    16321 tccttgcgat tcggatcgtc gctctgcttc gagagctcga cgagcgagcg acgcgcgagc
    16381 gtagcgccgt ccgattctgg agcgagctgt acggcgcgtt cgagcgcggg aatcgcctgg
    16441 cgaacgtcac cgcgttgcgt acgcgcatga ccgaggcgat acgacagcat cgcgcgatgt
    16501 gccgcctgcg tcgcggtgaa gcggtcgtcc ttcgccgtga gcaggcgcgt cagcatgccg
    16561 accgcagcgt cgaagtcggt ctgatcgagc gcgagcgtgg acgcgagatc gacggcgtcg
    16621 acatcgtacg gatcatgctc gacggcacgc tcggcggcgg cacgcgcgcg aatcttgtcg
    16681 ccggcggcga ggtaatcgtg cgcagcggac aagtgacgag cggcgcgatc cgcgggcgtg
    16741 gcgccgaatg ccgcgagcag ctcggcggtg cgcgcggagc ccgcgaggtc gccgtgcttg
    16801 ttctgcaggt cgagcagctt ctcgagcagt gggacgtgct ttgcctcgag cggctgcgcc
    16861 gattgcactg ccgccgcgta gcacgtgcga gcgcgctctt cgtccttcag catcatcagc
    16921 gacgtgtcgc cgagcgcttc gaacagcttg aggcgctctc ccggatcttc cgtcgcggtc
    16981 gcttggcgcg tgagcaggtc ggcggccttg cggtggtcgc cttgttccat cgcgatctcg
    17041 gcgagcgctt gcagcgcggg cccgtagttc ggcttcagct cgagcgcgcg ctcatacagc
    17101 gcgggcgcct tctccggacg cagcgagcga atctcggcga gcgcggcgtg atagagaccc
    17161 tgcgccactt cactcggata cttcgcggca tcttcgtgcg tcgcgagcgg cgacaggtgc
    17221 agtgcgctct cgcgccagcg ccgcgctttg tagcggttct cgccgagcgc gagcttgatc
    17281 aagagatgtc cgcggtgcag gcgatcggcg gccgtcagcg tctggtacgc gtcgtcgtcg
    17341 cggcctagtt tctcgtgcgc ggtcgcgagg cggcgatgca gatcggcaac gagcgcgccc
    17401 ggggcacccg tcagcgttgt tccgtcgaga atctcgcgca agaacgcctc gagatcgttc
    17461 caccgcagcg catcggcata gaagtcggcg agggcgacga tcgcggggat gtgatcgggc
    17521 accgtgccga gcgcttcgag gaggcgacgc tccgccgctg gctcgacacc cgctgcgcgt
    17581 gatagcgccg cgagctgcgt caggattcgc gccttctcgg gcggcgtcag tgattcgtcc
    17641 tcgagcttct tctcgaggat gtgctgcgcg ctctcggcat tgccgccttg ctccgagaga
    17701 ccggcgagcg cccacgttgc gtcggcatga taggggcgca gcgcgagcac gcgctcgtac
    17761 gccgactgcg cggccgcgag atcaccgacg cgctgctgga gcacttggcc cgccgccatc
    17821 agtgccgcga tcttgttgtc ttcgtcgcgc gcgctatccg cttcgcggag cttcgcatcg
    17881 gcaaaggcac gcggatcctc gtcgggcgac gacagctcgg cgagcaccga gagcgcggtt
    17941 ggatgctccg gcacgagcgc gagcgcttga tcgatcgcag cgcgcgcacc gggcttgtcc
    18001 tcctcggcgt tgcgcagctg cgcgaggcgg atgtagagca ccgcgagatc gccgcgcgat
    18061 gaccccttcg tcgttgcggt cggctgcgac ttgtcggtgc cttgcgcctt cggtccgggc
    18121 gccggacgct ttgcaccggg aggcggcttc gtcggagcgg tcgcaacagg ctccggcggc
    18181 gcgttcttca gctgctcggc aagctgctcg atacgaccct cgaggatcgc cgcggcttcg
    18241 cgcgagcgac cggccttcga cagcgccgcc accatttgat cggcaagcgc cggatcatcg
    18301 ggggccacgg tacgcgcgtg cttgagcgcg gctgccgctt cttcgggcga atcgagatgg
    18361 tcgatgagca catgcgcgcg acgggcgagc agcgcggatc gctccgcagg ctcacgcgcg
    18421 agggcggcac gcctacgaag cacgtccgcg agctcggccc agcgctgcgc gctctgatac
    18481 agctcgtcga gcgcacccca cgcgtcgatg tcgtcgggcc acgtcgtcac cgtttgcacg
    18541 tacgcgtcga tcgccttgcc cggctgctcg agctctttga cgtaaatgcg cgcgatcgcg
    18601 tgcagcgcct gccgcatgtc ctcggtgccc tcggcaatct ccgtgatgcg cgacagcgtc
    18661 tcgatcacct tcgaccaccg gccgacgtcc gcgtagagcg aagcgacctg attcaagatc
    18721 gcgatgtcgg caggcgcgag gttgcgcagg cgctcgagtg cgtcgagtgc gtcgtgcgtg
    18781 cgatgcagct tgtcgacgta aatcgcggcg agctcgcgga gcagctgtgg acgctcggat
    18841 tcgggggccg ctgtaccgag gcgcggatcg ctgcgctctt cgagcgacgc cgcgagctcg
    18901 acccagcgac cttcgttgcg atacagctct tcgagccgag agcgtgcggt cgcatcattg
    18961 ggcaacatcc cgaggatgcg gcgcagctgc gcttctgctt cgcgcggacg cttctgctcg
    19021 taacgaatct gcgcgacctc catgaggaga tccgcggcag cgacagcggt atcggcggac
    19081 tccgccattc cttcatagag gtcggcgagc cggtcccacg ctttgtgctc ttgcgcgacg
    19141 cgcatcaggc gagcgcgaac ttc
//
LOCUS       DATLBZ010000281         4296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1053849, whole genome shotgun sequence.
ACCESSION   DATLBZ010000281 DATLBZ010000000
VERSION     DATLBZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053849"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VMZ53_14820"
     CDS             <1..464
                     /locus_tag="VMZ53_14820"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HUS29774.1"
                     /translation="PDLVESTRDVWPRFVKNRSERFEARVVMLRIEQSPSLFFTGMQG
                     SRIPIASAHGEGRAELAGGAAQLEALEAQKLVAARFVDGKGEVTDLYPQNPNGSPNGI
                     ASVTTSDGRVTVIMPHPERVFRTVQLSWHPPDWGEDSPWMRMFRNARVWVG"
     gene            complement(466..1860)
                     /locus_tag="VMZ53_14825"
     CDS             complement(466..1860)
                     /locus_tag="VMZ53_14825"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HUS29775.1"
                     /translation="MRALAASIALVCGCAVEPGEPLGDPHGLPSCASPQVDQIFKAVR
                     SYIPKGSVTDQNFVTAANHADEHLYLDGPQIFPAMRELIAGAQRDVNLQTYVWEPGSD
                     PANEILDGLAELNRNRMYANATSPVTVRFLFDVSTTSYGSRVDALPLTWAAVEALKLD
                     PRFVKFELAGVARSATGALHKKTLVVDGKLAFITGANPQAHHNYAAPWRDAGYRLDGE
                     VALALQDDFDATWAAGMLWTCAGDVAGKWSACSAATQAIVREPVATTATAEADACQTM
                     LVATRVADSSPLANSTDNPSDQAFIAAMGAATDHVRIQTPNLNDDAAKKAIVDAVKRG
                     VRVDVVLSKGFNDSTEVYPGQGGTNEDNVQMLYDTLGAAGVPDKCDKLRFRWYTRNGV
                     VVDGNGLYASHAKYMSLDDSIVIVGTSNMDTQSWNNSREINVVVDDPAITQAWDRQMF
                     VPEMAAGEQVDYCK"
     gene            2055..3890
                     /locus_tag="VMZ53_14830"
     CDS             2055..3890
                     /locus_tag="VMZ53_14830"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29776.1"
                     /translation="MYPKSTLEPGTEAPLGRYRLLALLGQGGMADVYLACTQGPRNFT
                     KLLVVKLARFSGDPMFATMFLEEARLAAQLSHPNVVQTFEIGEEGSRYYIVMEYLDGG
                     VLSRLRQKCVKTGGIPLRTALTIMVHVLEGLEYAHEAVSIEGKQLKVVHRDLSPSNIM
                     VTAQGVVKILDFGIAKAADTQGFTQTGKFSGKLAYMPPEQLRGERVDQRADLFSAGVI
                     IAESALNEKLWGKSLDPEIASKLGQGLLPSLDREGKPIDPELRKIVDRALAIGRDERY
                     ASASEMKADLVKYLDSIGGLLPQKELADFVRKTIEDDRAKLQTVIDSQLARKSLQTWG
                     GETPLPSELPRIDRSGSRSKSPVILDDATVKSDVVVEDDEKELVVSRPSAPSSSKKPL
                     LFGVLGLAAVVAIGAIVVLGKNDDKPRAEPAALPAQPTQPTQPPTPQTVRLEVVASPS
                     DAQVALDGKPLGQNPYVGQLARDGNVHELTITAPGYRTMRQQITLERDASFRTSLEKE
                     VAVAPVPPVKTEEVATPPSTGKRTPTRVVTKKNTTATTAGTGTGSATIAVQPPPPPPP
                     DPKKSSTLDTDPYAATKKKRSLDSDVLDGGGSNKQKKQLENPWQK"
     gene            3890..>4296
                     /locus_tag="VMZ53_14835"
     CDS             3890..>4296
                     /locus_tag="VMZ53_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29777.1"
                     /translation="MARLLAVMVLLGAGAAAADDKIDEAKQHVQTGMQLYDENNFRAA
                     LVEFQRAYDIAPSYRIMYNIAQVQMELHDYAGALKAYTRYLKEGGPDVPAERQKEVNE
                     EIDRLKGRVGSVTIQTAPGAEILIDDVAVGFAPL"
BASE COUNT          790 a         1362 c         1399 g          745 t
ORIGIN      
        1 cgcccgatct cgtggagagc acgcgcgatg tgtggccgcg cttcgtcaag aaccgcagcg
       61 agcgattcga agcgcgcgtc gtgatgcttc gcatcgagca gagcccgtcg ctgttcttca
      121 caggcatgca gggctctcgc atcccgatcg cgagcgcgca cggcgagggc agggcagagc
      181 tcgcgggcgg cgcggcgcaa ctcgaagcgc tcgaagcgca gaagctcgtc gccgcgcggt
      241 tcgtcgacgg caaaggtgag gtcaccgacc tctacccgca gaaccccaat ggctcgccga
      301 acggcatcgc gtcggtcacc acatcggacg gccgcgtcac ggtgatcatg ccgcaccccg
      361 agcgcgtgtt ccgcacggtg cagctgtcgt ggcacccgcc cgactggggc gaggacagcc
      421 cgtggatgcg gatgttccgc aacgctcgcg tctgggtcgg ctgagctact tgcagtagtc
      481 gacctgctcg cccgccgcca tctcgggcac gaacatctgg cggtcccatg cttgtgtgat
      541 cgccggatcg tcgacgacga cgttgatctc gcgcgagttg ttccaggact gcgtgtccat
      601 gttcgacgta ccgacgatga cgatgctgtc gtcgagcgac atgtacttcg cgtggctcgc
      661 gtagaggcca ttgccgtcga cgacgacgcc gttgcgcgtg taccaacgaa agcgcagctt
      721 gtcgcatttg tcgggcacgc ccgcggcgcc gagcgtgtcg tagagcatct gcacgttgtc
      781 ttcgttcgtg ccgccttggc cgggatagac ctcggtcgaa tcgttgaagc ctttcgagag
      841 cacgacatcg acgcgcacgc cgcgcttcac cgcgtcgacg atggccttct tggcagcgtc
      901 atcgttgagg ttcggtgtct ggatgcggac gtggtccgtc gcggcaccca tcgctgcgat
      961 gaatgcttga tcggacgggt tgtcggtcga attggcgagt gggctcgaat cggcgacgcg
     1021 cgtcgcgacc agcatcgtct ggcatgcgtc ggcttctgca gtcgcggtcg tcgcaacggg
     1081 ctcgcgcacg atcgcttgcg tggctgccga gcacgcggac catttgcctg cgacatcgcc
     1141 cgcgcacgtc cacagcatgc ctgcagccca cgtcgcgtcg aagtcgtctt gcaatgcgag
     1201 cgcgacctcg ccatcgaggc ggtagccggc atctcgccac ggcgctgcgt agttatgatg
     1261 cgcttgtggg ttcgcacccg tgatgaatgc gagcttgccg tcgacgacga gcgtcttctt
     1321 gtgcagcgcg cctgtcgcac tacgcgcaac gcctgcgagc tcgaacttca cgaacctcgg
     1381 atcgagcttc agtgcttcga cggcggccca cgtcaggggc agcgcgtcga cgcgcgagcc
     1441 gtagctcgtg gtcgagacat cgaacagaaa gcggacggtg accggcgacg tcgcgttcgc
     1501 atacatgcga ttgcgattga gctccgcgag gccgtcgagg atttcgttcg ccggatcgct
     1561 gccgggctcc cacacatacg tctggaggtt gacgtcgcgc tgcgcgcccg cgatcagctc
     1621 gcgcatcgcc ggaaagatct gcggtccatc gagatacaag tgctcgtcgg cgtggttggc
     1681 cgcggtcacg aagttctgat cggtgacgga gcccttcggg atgtagctgc ggacggcctt
     1741 gaagatctgg tcgacttgcg ggctcgcgca cgatggaagg ccgtggggat cgccgagcgg
     1801 ttcacccggc tcgactgcac agccgcaaac caaagcgatc gaggctgcga gtgcgcgcat
     1861 ggtgcaagtg actgctcatc tcgtgccgaa cgatcatcgt tttccttacg cgtagttcgc
     1921 cgacgagtgg ctagttgcga agccggtcac tccaactttt tgtttgcagt caaagtcccc
     1981 acattggctc ctaaacttcg ctggtccgcg cctcttccgg tggagccaac tgcgcgcaaa
     2041 aataggctac cgagatgtat ccgaagagca ctctcgagcc tgggacggag gctccacttg
     2101 gccgctatcg ccttctcgct ctgctggggc agggcggaat ggcggacgtg tatctcgcgt
     2161 gcacgcaagg cccacgcaac ttcacgaagc tgctcgtcgt aaagctcgcg cggttctcgg
     2221 gcgatccgat gttcgcgacg atgtttctcg aggaagcgcg actcgccgcg cagctctcgc
     2281 atccgaatgt cgtccagacc ttcgagatcg gcgaggaagg ctctcgctac tacatcgtca
     2341 tggagtacct cgacggcggt gtgctgtcgc ggctccggca gaagtgcgtc aagaccggcg
     2401 gtattccgct gcgtacggcg ctgacgatca tggttcacgt gctcgaaggt ctcgagtacg
     2461 cgcatgaagc tgtgagcatc gagggtaagc agctcaaggt cgtgcatcgc gatctgtcgc
     2521 cctcgaacat catggtgacc gcgcagggcg tcgtgaagat cctcgacttc ggcatcgcga
     2581 aggccgctga tacgcaaggc ttcacgcaga ctggcaagtt cagcggcaag ctcgcgtaca
     2641 tgccgcccga gcagctgcgc ggcgagcgcg tcgatcagcg tgcggacctg ttctccgcag
     2701 gcgtgatcat cgcggagtcc gcactgaacg agaagctgtg gggcaagtcg ctcgatccgg
     2761 agatcgcgtc gaagctcggg caggggctgt tgccgtccct cgatcgcgag ggcaagccga
     2821 tcgatcccga gctgcgcaag atcgtcgatc gcgcactcgc gattggccgc gatgaacgct
     2881 acgcgtccgc gagcgagatg aaagccgacc tcgtgaagta tctcgactcg atcggcggcc
     2941 tactccccca gaaggagctc gcagacttcg tgcgcaagac gatcgaggac gatcgcgcga
     3001 agctgcagac cgtcatcgac tcgcagctcg cgcgcaagag cctccagacg tggggcggcg
     3061 aaacgccgct gccgagcgag ctgccgcgta tcgatcgctc gggaagccgc agcaagagtc
     3121 ccgtcatcct cgacgacgcg acggtgaaga gcgatgtcgt cgtcgaggac gacgagaagg
     3181 agctcgtagt ctcgcgtccc agcgcgccat cgtcgagcaa gaagccgctg ctgtttggcg
     3241 tgcttggtct cgcggccgtg gtcgcgatcg gcgccatcgt cgtcctcggg aagaatgatg
     3301 acaagccgcg cgccgagccc gcagccctcc cggcgcagcc gacgcagccg acgcagccgc
     3361 cgacgccgca gacagtgcga ctggaggtcg ttgcgagccc ctccgacgcg caagtcgcgc
     3421 tcgacggcaa accgctcggg caaaacccgt atgtcggaca gctcgcacgc gatggcaatg
     3481 tccacgagct cacgatcacc gcgccgggct atcgcacgat gaggcagcag atcacgctcg
     3541 agcgcgatgc gagctttcgc acgagcctcg agaaagaggt cgctgtcgcg ccggtaccac
     3601 ctgtgaagac cgaggaggtc gcgactccgc cgtcgactgg caagcgaacg ccgacgcgcg
     3661 tcgtgacgaa gaagaacacg accgcgacga cagcgggcac ggggacgggg tccgcgacaa
     3721 tcgccgtaca gccaccgccg cctccgccgc ccgatccgaa aaagtcctcg acgctcgaca
     3781 ccgatccgta cgccgcgacg aagaagaagc ggtcgctcga cagtgacgtg ctcgacggtg
     3841 gtggctcgaa caagcaaaag aagcagctgg agaatccatg gcaaaagtga tggctcggtt
     3901 acttgcagtg atggtgctgc tcggcgccgg tgcagcagcc gccgacgaca agatcgacga
     3961 agcaaagcag cacgtgcaga ccggcatgca gctctacgac gagaacaact tccgcgctgc
     4021 gctcgtcgag ttccaacgcg cgtatgacat cgctccgtcg tatcgcatca tgtacaacat
     4081 cgcgcaggtg cagatggagc tgcacgacta tgccggcgcg ttgaaggcgt acacgcgcta
     4141 tctcaaagag ggcggcccgg acgttccggc cgagcgccag aaggaagtca acgaagagat
     4201 cgaccgcctc aaaggacgcg tcggctcggt gacgatccag actgcgcctg gcgctgaaat
     4261 cctcatcgat gatgtcgccg ttggtttcgc gccgct
//
LOCUS       DATLBZ010000282         4536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7604802, whole genome shotgun sequence.
ACCESSION   DATLBZ010000282 DATLBZ010000000
VERSION     DATLBZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7604802"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VMZ53_14840"
     CDS             <1..1066
                     /locus_tag="VMZ53_14840"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUS29778.1"
                     /translation="IADAIRYAADNGAKVINMSLGGAFPSNVLKKAVAYAHAKGVTVV
                     CAAGNESRGKVGYPAAYPGAIAVSATQNDEAITFYSNYGKDIDIAAPGGNTRDSSGGR
                     NNPDGGVLQNTIKIQDPTKAEYAAYMGTSMASPHAAGVAALVVGEGISDPDMVEKILK
                     DSARKPQNQKYTTDKYGAGIIDAPAAVLKARASGGGWQLGLGLLMAGAVAASARRRGL
                     GTKLGAGYIAGVVVGASGLFFLPYIAPQLSSMPGIHTLTHGLPSWDLGLLGPSGHGNA
                     LFFSALVPLGLLALGYGVPKARAPLAGLAVGVAAHLAFFAVVPMTSVQYVPAAFGLAS
                     MWLVLNAVICLALARLALRR"
     gene            1104..1844
                     /locus_tag="VMZ53_14845"
     CDS             1104..1844
                     /locus_tag="VMZ53_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29779.1"
                     /translation="MRKLAATLFVLSAPALAYADDAVKRDASLSADTATFLPFTDSPG
                     SQSHMVKTLGVYDGARGGAALDTTVEAKLSEKFQLEAQLEVEAGEASPSIEGQFDLLE
                     EKQHGIDLQAAVGVDASGFNEVPAVFAKATVGGHVSGTYLVGDSQFELGTQRGERAAV
                     LGFAGIRDLGGHLYMGFDSRLRVDLEKDMSEPEGEASWDLVAGPLATYATGRYAITAA
                     LGLSGTEPRDAMTSDVGAFASLGVGAVF"
     gene            1864..2961
                     /locus_tag="VMZ53_14850"
     CDS             1864..2961
                     /locus_tag="VMZ53_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29780.1"
                     /translation="MRLALLLVAVTGCGRIGFDTPGDGNNDSQASLALTCNKPVRLVQ
                     PAELVYPRLAGIATQQRIGAAWIEKSDAIVSGGAAFETDELLANPGTTVFTPGSVGYT
                     AVSLASNGPTFIGAGAINDATVFRLYDSASLQPIAPDLTDSTVIQNPHAITSVDAASG
                     KSYAFVGSDPAGNVNLYGISATNQISGGVVIGFQDSYIGITTMGARFTIAIVSTDSGQ
                     CELASVAVDFGDTGPLIPFGGAECVQPTMAYAPGRSDFLLLTYDTALDTILQRITTIS
                     GSTLTPGAPTDFGGGTQPRAVATPSGYWATYHVASTVRAKHLSFAGVEDFDVFLGAAP
                     DDTSHDLVTRKGEAYALWMSDALYVAHLCAN"
     gene            complement(2958..3443)
                     /locus_tag="VMZ53_14855"
     CDS             complement(2958..3443)
                     /locus_tag="VMZ53_14855"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HUS29781.1"
                     /translation="MKHRIGIAREQYKFSCAHMTVFPDGTKERLHGHNYTIAVALEVD
                     TVELSAMIPFAPIKSALSEICAAWKERVLLAAHNPRFELVRDESEIEFRLCGERYVLP
                     RGDALLLPIDNISVEALAAHVASLLHTKLGPLLGAHVHSIEATVEESPGQGASCRVEL
                     R"
     gene            complement(3495..4460)
                     /locus_tag="VMZ53_14860"
     CDS             complement(3495..4460)
                     /locus_tag="VMZ53_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29782.1"
                     /translation="MRGLVAVALLAGCGGASASGSASISIHDDLAVAPTALQACASGD
                     INAQMQIVADLRDSYHEMIVCGGLSMQFNNSITSVIANALLQKAMGTPLQYQGNGVYA
                     TPNGVMWIRVSTDAGPLSFNALDPQSYLAGLTVTVNAQGMVGGAMQGGGGWGSMLGRA
                     AGKADVQVSFQGEGPGFNLLGITPQEARSGRLDQYLKRIEHSLGSLIRVENRVTVNNQ
                     HGGTTIQYLLQSPPTPIVDMSKKNAVPMQLVSITATNQLGQQVSITKWTMDYKGDGGK
                     VLDGTIGMDIAGGTFPYHVEMTYPHRAEPDITLSCGNAGAVAPIQ"
BASE COUNT          764 a         1473 c         1495 g          804 t
ORIGIN      
        1 catcgcggac gcgattcgct acgcagccga caacggcgcg aaggtcatca acatgtcgct
       61 cggcggcgcg ttcccgtcga acgtgctgaa gaaggcggtc gcctacgcgc atgcgaaggg
      121 cgtcaccgtc gtgtgcgccg ccggtaacga gagccgcggc aaggtcggct atccggccgc
      181 atatcccggc gcgatcgcag tcagcgcgac gcagaacgac gaagcgatca cgttctactc
      241 gaactatggc aaggacatcg acatcgcagc accgggcggc aacacgcgcg actcgagcgg
      301 tggtcgcaac aacccggatg gcggcgttct ccagaacacg atcaagatcc aggacccgac
      361 gaaggcggag tacgccgcgt acatgggcac gtcgatggcg tcaccacacg cagcgggcgt
      421 tgcggctctc gtcgtcggtg aaggcatcag cgatcccgac atggtcgaga agatcctgaa
      481 agacagcgct cgcaagccgc agaaccagaa gtacacgacc gacaagtacg gcgcgggcat
      541 catcgatgcg ccggcagcgg tcctcaaggc gcgtgcatcg ggcggcggtt ggcagctcgg
      601 tctcggcctc ctcatggcgg gcgcggttgc tgcgagcgcg cgtcgtcgcg gtctcggcac
      661 gaagctcggc gcgggctaca tcgctggcgt cgtcgtcggt gcgtcgggcc tcttcttcct
      721 gccctacatc gcgccgcagc tgtcgtcgat gcctggcatc cacacgctga cgcacggcct
      781 gccgtcgtgg gacctcggcc tcctcggacc gtcgggtcac ggcaatgcgc tgttcttcag
      841 cgcgctcgtc ccgctcggtc tcctcgcact cggttatggc gtcccgaagg cgcgcgcgcc
      901 gcttgctggc cttgcagtcg gtgttgccgc gcatctcgcg ttcttcgcgg tcgtgccgat
      961 gacgagcgtg cagtacgtgc cggcggcctt tggcctcgcg tcgatgtggc tcgtgctgaa
     1021 cgccgtgatc tgtctcgccc tcgcgcgcct cgcgctgcgt cgctaagcga atcactggca
     1081 catcgctcgc agaagagcgt ggtatgcgaa agctcgctgc cacgctcttc gtactttcgg
     1141 cgcccgccct cgcgtacgcg gacgacgctg tcaaacgcga tgcgagcctc agcgcggaca
     1201 cggcgacgtt tcttccgttc accgactcgc ccggcagcca gtcgcacatg gtgaagacgc
     1261 tcggcgtcta cgacggtgcg cgcggtggcg cggcgctcga taccaccgtg gaagcaaagc
     1321 tctcagaaaa attccaactc gaggcgcagc tcgaggtcga ggcaggcgaa gcgtcgccga
     1381 gcatcgaagg tcagttcgac ctcctcgaag agaagcagca cggaatcgat ttgcaggccg
     1441 cggtcggcgt cgacgcgagt ggcttcaacg aagtgccggc ggtcttcgcg aaggcgaccg
     1501 taggcggtca cgtcagtggc acgtacctcg tcggcgactc gcagttcgag ctcggcacgc
     1561 agcgcggcga gcgtgcggcg gtcctcggct tcgcgggcat tcgcgatctc ggcggccacc
     1621 tctacatggg cttcgactcg cggctgcgcg tcgatctcga gaaggacatg agcgagcccg
     1681 agggtgaagc gtcgtgggat ctcgtggcag gtccactcgc gacgtatgca acgggccgct
     1741 acgcgatcac cgcggcactc gggctctcgg gcaccgagcc acgcgatgcg atgacgagcg
     1801 acgtgggtgc gttcgcctcg ctcggcgttg gcgcggtgtt ctgaacccac gtatcgtgga
     1861 ggcgtgcggc tcgcgttgtt gctcgttgcg gtcacggggt gcggccgcat tgggttcgac
     1921 acgccgggcg acggaaacaa cgactcgcaa gcgtcactcg ctctgacgtg caacaagccg
     1981 gtgcgcctcg tccagccagc ggagctcgtg tatccgcggc tcgcggggat cgcgacgcag
     2041 cagaggatcg gcgcggcgtg gatcgagaaa tcggacgcga tcgtctccgg cggcgccgcg
     2101 ttcgagaccg acgagctgct ggcaaatcca gggacgacgg tattcacgcc agggagcgtc
     2161 ggctacaccg cggtgtcact cgcgtcgaac ggtccgacgt tcatcggcgc cggcgcgatc
     2221 aacgacgcca ccgtgttccg gctctacgac tcggcgtctc tacagccgat cgcgccggat
     2281 ctcaccgaca gcaccgtgat ccagaatccg cacgcgatca cgtcggtcga cgccgcgagt
     2341 ggcaagagct acgcgttcgt cggcagcgat ccggcaggca acgtgaacct gtacggcatc
     2401 tctgcaacga accagatctc cggcggtgtc gtcatcggat ttcaagacag ctacatcggc
     2461 atcacgacga tgggcgctcg gttcaccatc gccatcgtca gcacggatag cgggcagtgc
     2521 gagctcgcgt ccgttgcggt cgacttcggc gacaccggac cgctcatccc attcggtggc
     2581 gcagagtgcg tgcaaccaac gatggcatac gcgcctggcc gctcggactt cctgctgctc
     2641 acgtacgaca cggcgctcga cacgattctc cagcgaatca cgacaatcag tggctcgacg
     2701 ctcacgccgg gcgcgccgac ggacttcggt ggcggcacgc aaccgcgtgc agttgcgaca
     2761 ccgagcggat actgggcgac ctatcacgtc gccagcaccg ttcgcgcgaa gcacctgtcg
     2821 tttgccggcg tcgaagattt cgatgtgttc ctcggcgcag cgcccgacga cacgagccat
     2881 gacctcgtca cgcgcaaggg cgaggcgtac gcgttgtgga tgtcggacgc gctctacgtc
     2941 gcgcatctct gcgcgaatta gcgcagctcg acgcggcagc tggcgccctg gccgggactc
     3001 tcttcgacgg tggcttcgat cgaatggacg tgcgcgccga gcaggggccc cagcttggtg
     3061 tgcaagagcg atgcgacgtg cgcggcaagc gcttcgacgg agatgttgtc gatcggcagg
     3121 agcagcgcat caccacgcgg cagcacgtag cgctcgccac acagccggaa ctcgatctcg
     3181 ctctcgtcgc gtacgagctc gaagcgcggg ttatgagcgg cgagcaggac gcgttctttc
     3241 cacgcggcgc agatctcgga cagcgcactc ttgatcggcg cgaatggaat catcgccgac
     3301 agctcgacgg tatcgacttc gagcgcgacg gcgatcgtgt agttgtgacc gtgcaggcgc
     3361 tctttggtgc cgtcgggaaa caccgtcatg tgcgcgcacg agaacttgta ttgttctctg
     3421 gcgatgccga tgcggtgctt catggccgaa ccataccgcg tcatcgcgcg accgtcgtgg
     3481 tcgtgggcgt agcgctactg gatcggcgca acagcgccag cgttgccgca cgagagcgtg
     3541 atgtccggct cggcgcggtg cggatacgtc atctcgacgt gatacgggaa cgtgccgccg
     3601 gcgatgtcca tgccgatggt tccgtcgaga accttccctc cgtcgccctt gtagtccatc
     3661 gtccacttcg tgatcgagac ctgctgaccg agctggttcg tcgcggtgat cgagacgagc
     3721 tgcatcggga ccgcgttctt tttgctcatg tcgacgatcg gcgtcggcgg gctctgcaac
     3781 agatattgga tcgtcgtgcc gccgtgttgg ttgttgacgg tgacgcggtt ctcgacgcga
     3841 atcagcgagc cgagtgagtg ctcgatcctc ttgaggtatt gatcgagccg gccggacctc
     3901 gcttcctgcg gcgtgatgcc gagcaagttg aagcccgggc cttcgccttg gaagctgacc
     3961 tgcacatcgg ctttgcccgc ggcacgcccg agcatcgagc cccaacctcc gccgccttgc
     4021 atcgcgccgc cgaccatgcc ctgcgcgttg acggtgacgg tgaggccggc gaggtagctc
     4081 tgcggatcga gcgcgttgaa cgagagcggc cctgcgtcgg tgctcacgcg tatccacatc
     4141 acgccgttcg gcgtcgcgta gacaccgttg ccttgatact gcagcggcgt tcccatcgcc
     4201 ttctgcaaga gcgcgttcgc gatcacgctc gtgatcgagt tgttgaactg catcgagagg
     4261 ccgccgcaca cgatcatctc gtgatagctg tccctcaggt ccgcgacgat ctgcatctgc
     4321 gcattgatgt cgcccgacgc gcacgcttgc agagcggtgg gagcgaccgc gaggtcgtcg
     4381 tggatggaga tcgaagcgct gccactggcg gacgcaccgc cacagcccgc gagaagtgcc
     4441 actgcaacta agccacgcat gttcagacca tcaacgcacg aatggtgccg tgcttccagt
     4501 ctgtggctaa ctcgcgtgat ctcgtcgagg gtagcg
//
LOCUS       DATLBZ010000283         2392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4776228, whole genome shotgun sequence.
ACCESSION   DATLBZ010000283 DATLBZ010000000
VERSION     DATLBZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4776228"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..776)
                     /locus_tag="VMZ53_14865"
     CDS             complement(135..776)
                     /locus_tag="VMZ53_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HUS29783.1"
                     /translation="MTTLVQLRRHVARLRTCGDCPDMIGPSVSGGAVMSPVMLIGQAP
                     GVKEGPAGKPFAWTAGKTMFGWFATLGLDEEQFRARVYMAAVCRCFPGKAASGGDRVP
                     SPEEIAKCSRHIQAEIKILQPRLVIPVGKLAIAQLYPELDKLTEIIGSTRRGTLHGHE
                     FDVIALPHPSGASTWHRTEPGKSLLDKALRTIGKHEAWRSLQAAPAPSAEASL"
     gene            900..1490
                     /locus_tag="VMZ53_14870"
     CDS             900..1490
                     /locus_tag="VMZ53_14870"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HUS29784.1"
                     /translation="MTTLEHEQLIRRVLGTNYLSLSLAKLTVEEDPKDGASVKVLVNE
                     GEQPSAVEGKGVGVVDALWAALLDRYAREYQSLKTIQLVGFQVGADMDTKKAQAGVDA
                     IGRVTLDVTNSEGRHFTFSDQSRSIMTSTARAVVACVQYFVNAERAFLTLHNARRDAM
                     ARGREDLVARYTAEMAEVVEATSYAEVIANIRKELG"
     gene            1501..>2392
                     /locus_tag="VMZ53_14875"
     CDS             1501..>2392
                     /locus_tag="VMZ53_14875"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HUS29785.1"
                     /translation="MKRGFALALLLVVACAVRCETCRDSPRAPVRVATFNIENFPQSR
                     AQIEGAFAELRDIHAGIIAVQEIGDPDLFAREARQRLGDEWRFVSVDSRPIGERRPGH
                     HLGVLFDSSTWKLLATRAHDDTRLAGGRHKPVLDVRLQRGSLKLHVLVVHLKSNTEGR
                     PIRAAQYAALRDVIREVQRAKERIVLMGDFNATEAGDRDDLALLARDTHMTWASEPLA
                     CSAFWAREDGCPRSRLDHVLTWLPTSRSSLSSASSPSSSSPTSPSSSLSLASPTIEVA
                     GACAREGCEWQQSCPVYARQV"
BASE COUNT          419 a          811 c          759 g          403 t
ORIGIN      
        1 agaggcgtcg ctcgttgccg gcggtgccgt cgggtcccgc ttggtgcgtc ggaatggcga
       61 ccagctcgga caacagctcg gcgacacggc tcatgtcgcg gaccgtatca cttcagcgct
      121 tctcagaggt tcgatcagag cgaggcttcg gcactcggcg cgggagccgc ttgcagcgag
      181 cgccacgcct cgtgcttgcc gatcgtgcgc agcgctttgt cgagcagcga cttgccgggc
      241 tccgtgcgat gccacgtcga tgcgcccgat ggatgtggca gcgcgatgac gtcgaactcg
      301 tgaccgtgaa gcgtgccgcg ccgcgtgctg ccgataatct ccgtcagctt gtcgagctcg
      361 ggatagagct gcgcgatggc gagcttgccg acgggaatca cgaggcgcgg ctggagaatc
      421 ttgatctccg cctggatgtg acggctgcac ttcgcgattt cctcgggcga aggcacacga
      481 tcgccgccgc tcgccgcctt gcccggaaag cagcgacaca cagccgccat gtacacacgt
      541 gcgcggaact gttcctcgtc gaggccgagt gtcgcgaacc agccgaacat cgtcttgccg
      601 gcggtccatg cgaatggctt tccagccggt ccttctttga cgccgggcgc ttggccgatc
      661 agcatcaccg gcgacatcac ggcgccgccg ctcaccgatg gcccgatcat gtccggacag
      721 tcaccgcacg tgcggaggcg tgcgacgtgg cgccgcagct ggactagcgt ggtcatcgtg
      781 cgcagccact atggcagccg tatgtgacat cgcaacactc gggagtatgc gtaaccatct
      841 acagatccca ccgatctaga tcacgggcag gcacccgaaa cccacgctaa gatctcgaag
      901 tgaccaccct cgagcacgag cagttgatcc gtcgggtcct cggcaccaac tacctctcgc
      961 tgagcctcgc aaagctcacc gtcgaggaag atccgaaaga cggcgcctcg gtgaaggtgc
     1021 tcgtcaatga aggcgaacag ccgagcgcgg tcgagggcaa gggcgtcggt gtggtcgatg
     1081 cgctctgggc cgctctcctc gatcggtacg cacgcgagta ccaatcgctc aagacgatcc
     1141 aactcgtcgg cttccaagtc ggcgccgaca tggacacgaa gaaggcccaa gcaggcgtcg
     1201 acgccatcgg tcgcgtcacg ctcgacgtga cgaactccga aggccgtcac ttcacgttca
     1261 gcgatcaatc gcgttcgatc atgacgtcca cggcacgcgc cgtcgtcgct tgcgtccaat
     1321 acttcgtgaa cgcagagcgc gcattcctca cgctgcacaa cgcacgacgt gatgcgatgg
     1381 ctcgcggccg cgaagacctc gtcgcgcgct acaccgccga gatggccgaa gtcgtcgaag
     1441 cgacgagcta cgccgaagtg atcgcgaaca tccgcaaaga gctcggctaa gctccgccgc
     1501 gtgaaacgcg gattcgcgct cgcgctgttg ctcgtcgttg cgtgcgcggt ccgatgcgag
     1561 acatgccgtg actcaccacg cgcacccgtc cgcgtcgcga cgttcaacat cgagaacttc
     1621 ccgcagtcgc gcgcgcaaat cgaaggcgcg ttcgccgagc tgcgcgatat ccacgccggc
     1681 atcatcgccg tccaagaaat cggcgacccc gatctgttcg cgcgcgaagc tcgccagcgc
     1741 ctcggcgacg agtggcggtt cgtcagcgtg gactcgcgtc cgatcggcga gcggcgtcca
     1801 ggtcatcacc tcggcgtgct cttcgactcc tcgacgtgga agctgctcgc cacgcgcgcg
     1861 cacgacgata cgcgtctcgc gggtggtcgc cacaagccag tgctcgacgt ccgcctgcaa
     1921 cgcggctcgc tgaagctcca cgtgctcgtg gtccacctca agtcgaacac ggaagggcgc
     1981 ccgattcgcg ccgcgcaata cgccgcgctc cgcgacgtaa tccgcgaggt gcagcgcgca
     2041 aaagagcgca tcgtcctcat gggcgatttc aatgcaaccg aagcgggcga ccgcgacgac
     2101 ctcgcgctac tcgcgcgcga tacgcacatg acgtgggcaa gcgagccgct cgcatgcagc
     2161 gcattctggg cgcgcgaaga cggctgcccg cgctcgcgcc tcgaccacgt gctgacctgg
     2221 ctcccgacgt cgcgctcgtc gttgtcgtcg gcgtcgtcgc cgtcgtcatc gtcgccgacg
     2281 tcgccgtcgt catcgttgtc gttggcgtcg ccgacgatcg aggttgctgg cgcgtgcgcg
     2341 cgtgaaggtt gcgagtggca gcagagctgc ccggtgtacg cacgccaggt ct
//
LOCUS       DATLBZ010000284         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1804439, whole genome shotgun sequence.
ACCESSION   DATLBZ010000284 DATLBZ010000000
VERSION     DATLBZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804439"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VMZ53_14880"
     CDS             complement(<1..1019)
                     /locus_tag="VMZ53_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS29786.1"
                     /translation="MQPAKILVADDEQNLRRVLTALLRRDGHDVVQAGSGLEAIEHLG
                     QVDVVITDLRMPGADGMEVLRTASAKHPHVPVIMITAYGSVGQAVEAIKAGAFDYIEK
                     PFEQDAIRMVIEKAIGQAAANKLAPQPTLYLSSESGPSEKSKFGLVGMSPEMHGIFAV
                     IEKVADTPSTVLITGESGTGKELVAKALHEHSSRKNGPFIKINCAAIPKTLMESELFG
                     YEKGAFTGATSSKPGRFELADRGTLFLDEIGEIPVEMQVKLLRAIQESEFERVGGIKT
                     IKVDVRLITATNRDLELEIQKGNFREDLFYRLNVVPLQIPPLRKRTGDIPLLVGHIIK
                     KFNERL"
     gene            complement(1036..>2365)
                     /locus_tag="VMZ53_14885"
     CDS             complement(1036..>2365)
                     /locus_tag="VMZ53_14885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS29787.1"
                     /translation="RIMTALEESRRVTDAAVYLLDADGAGFDRAGFHGQAPPERLDAN
                     GERALLDRVRTGYLDVDHLQQELDDIEQGPDVEARRTALMALKARVEELQATLIFPLF
                     GSAETEQGPWLLGLFCVRDDRTNSAFDADDIDTFRQLAIAAARVIESSQAYERVKERD
                     RLAALGEMAAGLAHEIRNPLGAIKGAAQLLMTSDGQPAPQHSENAEFLQIIVEEANRL
                     NNVVTRFLDYARAERPGREGADKVDLNNVVRKTVQLLQKEQHPKIDLRVRTDDQLPQV
                     AGDPDSLLQVFLNLGQNALQAMPDGGTLEILTTRRRRSRLGYGQFCEVRFRDTGHGIA
                     RDRLKKLFIPFYTTKQKGTGLGLAISHRIINQHGGTIEVRSTIGQGSTFSVFLPAAEP
                     VPASKVEDITETGRLTPVGDEAAAEKSAEAEDDSLTPLPAAEPAPSTSTG"
BASE COUNT          352 a          742 c          808 g          463 t
ORIGIN      
        1 aggcgctcgt tgaacttctt gatgatgtgg ccgacgagca gcgggatgtc gcccgtgcgc
       61 ttgcgcagcg gtggaatctg cagcggcacg acgttgaggc ggtagaacag atcctcgcgg
      121 aagttgccct tctggatctc cagctcgagg tcgcggttcg tcgccgtgat caggcggacg
      181 tcgaccttga tcgtcttgat gccgccgacg cgctcgaact cgctctcctg gatcgcgcgc
      241 agcagcttga cctgcatctc gacggggatt tcgccgatct cgtcgaggaa cagcgtgccg
      301 cgatcggcga gctcgaaccg gccgggcttg ctcgatgtcg cgccggtgaa cgcgcccttc
      361 tcgtagccga atagctcgct ctccatgagc gtcttgggaa tcgcggcgca gttgatcttg
      421 atgaaaggac cgttcttgcg gctcgagtgc tcgtgcagcg ccttcgcgac gagctccttg
      481 ccggtgcccg actcgccggt gatgagcacc gtcgacggcg tgtcggcgac tttctcgatt
      541 accgcgaaga tgccgtgcat ctcgggcgac atgccgacga ggccgaactt gctcttctcg
      601 ctcggcccgc tctcgctcga cagatagagc gtcggctgcg gtgcgagctt gttcgcggcg
      661 gcttggccga tcgccttttc gatcaccatg cgaatcgcgt cctgctcgaa cggcttctcg
      721 atgtaatcga acgcgcccgc cttgatcgct tcgaccgcct gaccgacgga gccgtacgcg
      781 gtgatcatga tgaccggcac gtgtggatgc ttcgccgatg cggtgcgaag cacctccatg
      841 ccgtcggcac cgggcatgcg caaatccgtg atcacgacgt cgacctgacc gaggtgctcg
      901 atcgcttcga ggccgcttcc tgcctggacc acgtcgtgac catcgcgacg caacagcgcc
      961 gtgagaacac gacgcaggtt ctgctcgtcg tcggcaacga ggatcttggc gggctgcatg
     1021 ggtggccgcg ctactctacc ctgtactcgt gctgggtgcg ggctccgcgg ccggcagtgg
     1081 cgtcaaggag tcgtcctcgg cctctgcgga cttttcggcg gccgcctcgt cgccgacggg
     1141 tgtaagccgt cccgtctcgg tgatgtcctc gaccttgctc gcgggcaccg gctcggcggc
     1201 gggcaggaag acggagaacg tcgagccctg accgatcgtc gagcgcactt cgatcgtgcc
     1261 gccgtgctgg ttgatgatgc gatggctgat cgcgaggccg aggcccgtgc ccttctgctt
     1321 cgtcgtgtag aacgggatga acagcttctt gaggcgatcg cgcgcgatgc cgtgaccggt
     1381 gtcgcggaac cggacctcgc agaactggcc gtagccgagg cggctgcgcc ggcgacgcgt
     1441 cgtgaggatc tcgagcgtgc cgccatcagg catcgcctgc agcgcgttct ggccgaggtt
     1501 caagaacact tgcagcagcg agtctggatc gccggcgacc tgtggcagct gatcgtcggt
     1561 gcgcacgcga agatcgatct tcggatgctg ctccttctgg aggagttgga ccgtcttgcg
     1621 cacgacgttg ttgagatcga ctttgtcggc gccttcgcga cccgggcgtt ctgcgcgcgc
     1681 gtaatcgagg aagcgcgtca cgacgttgtt cagccggttc gcttcctcga cgatgatctg
     1741 gaggaactcg gcattctcgg agtgctgcgg ggcgggctgg ccgtcggatg tcatgagcag
     1801 ctgcgcggca cctttgatcg cgccgagcgg attgcgaatc tcgtgcgcga gaccagcggc
     1861 catttcaccg agggcggcga ggcggtcgcg ttctttgacg cgctcgtatg cttgcgacga
     1921 ttcgatgacg cgcgcggcgg cgatcgcgag ctggcggaat gtatcgatgt cgtcggcgtc
     1981 gaacgcgctg ttcgtacgat cgtcgcgcac gcagaacagg ccgaggagcc acgggccttg
     2041 ttccgtctcg gccgagccga acagcgggaa gatcagcgtc gcctgcaatt cctcgacgcg
     2101 cgccttgagc gccatcagtg cggtgcggcg ggcctcgacg tcgggacctt gctcgatgtc
     2161 gtcgagctcc tgctgcagat gatcgacgtc gaggtagccg gtgcggacgc gatcgagcag
     2221 cgcgcgctcg ccattcgcgt cgaggcgctc cggcggtgcc tggccgtgaa agcccgcgcg
     2281 atcgaagccc gcaccatcgg cgtcgagcag gtagaccgcc gcgtcggtga cgcgtcgcga
     2341 ttcttcgagc gcggtcatga tgcga
//
LOCUS       DATLBZ010000285        13956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5533709, whole genome shotgun sequence.
ACCESSION   DATLBZ010000285 DATLBZ010000000
VERSION     DATLBZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5533709"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4336
                     /locus_tag="VMZ53_14890"
     CDS             <1..4336
                     /locus_tag="VMZ53_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HUS29788.1"
                     /translation="NLPPAGGAPRASAESGGTATEVDTETDTTETLKLEAFAITPNGT
                     YAAAKVGKTLHVWNVSSGDQVGVAELGCQVAIDPSGIDLDTPCNERNLGSVIDLQISP
                     DGAWIIVVGQSAAELFHRTKDALVPAPVPAGGTAHCRLKPDDELYSYERSRLGFSAAF
                     SGNSSVLAVGRGRSIDTWRLDGTAPTRIETICANAESLALDFGGTWLARRNDIERSRC
                     YYETTKPCGFFLYVLRDDRWIGFSSQHIRAFDSHQAGVALEPSKIGFSRTDLAAHSSS
                     PVRTSCDYDSYFLSQGTAVSASSCGYVVDDGKSVIAGVLPLGTTLAVAPLARVVADAD
                     DGTVRIWKLSRSLENVVQTTADATPLAKTSIAPGGVWSGTIAVARDGLTLAIGSSDTA
                     SDSREQIVDGKPPVLALFSTVESAEKTELVRTSGEMPQLLDKNGNHGVAYVDFDPAQE
                     RVYSLGRDGHVRIWRRSDGSKLLDLGNEQGRILAAGFTPDGSKIATGDHVGRLCAWNS
                     ATGQKLACSEPTNVALRRGAFAMTPAGAFITTDVKGTIRLWKLGAEAPIVVGNHALAV
                     DRLEVDKTGKWLASASNDGTARLWDLTTCAPSGCKESLRLEDPSGFDDVLLPSDNGTT
                     WLIATPHDDRVVVVDLAKPAERHVLTTRNWASSVDVSRPGHLLLCEFGGTVEEWDLRT
                     KRLSGRLLPSDDRAWCGAKYVGENRFITQFTNGEVALWSIDALARGLTGWSTMGGLSF
                     DASGAIVGTPRLSSGARDVLVMSRDGNVTARVPAVSRAVVDDKAIIFGTPNGGIGVVD
                     AAGNGALSSGTDYVASTIAASHGERLVVTWDLHYELAPSASVQAPVWRALPSSLPHGS
                     VRDARLAAGSWIVAAAQDDSVTRYDATGHRNDTSTLKGAAHVVEADNGDGLLVVGDFD
                     VATIGATGPPVVHKMMSGVYSRAAAWDVGASLLALASRNNVVEIRDLSTNKVLWSESK
                     TFGTDKYYAQLIKFGTVGTQRVIALATEGYIGIYAVATGKLVQEFLEKTKQKGDVLDL
                     WFLEDGNLLTHDDAARLVLWDSRTGKQLEMLQDINDVVLGPGNRFLVTRWGEMRYVTY
                     KNKKLDLSDPHLDCGRYVYRIASSATGKLFAAASQPKNETDSAQLCLIDAATGRVVTT
                     WSSKQTQITRLAFRRDDTALLSIADEPAPVLWEVPTGKQLQVFEHEDLLVAGFAKGTP
                     YIVTADTSGKLRIYDARAGRMVAESTQPPGAVQVVSSGLIDETSGILTTTDARGVIVQ
                     IDLATGRRLREFSTQRREIRAIAVAPGGSTIAAAVGSEVQLWRNAPGLLVPVALPPIT
                     LDTGEVDGLSFSPDGRWLAAADGFSVENTEAEHGLLTLWDASDGSNLLRKDTPVRLTS
                     VAWSNDAREVVVAGNAGYLQVVSFGLEQRPVEVVRKILERMAPRTTYHPTQSPRKPST
                     SGGGMIDI"
     gene            complement(4333..5496)
                     /locus_tag="VMZ53_14895"
     CDS             complement(4333..5496)
                     /locus_tag="VMZ53_14895"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HUS29789.1"
                     /translation="MRASDLAAEVRATEYSGTTLGADLDRLERVAGVDAPEAAVFYAG
                     RILEVLIAEEVRALGGEPSNNVYGNLVRLSELGVIPEFPARLAHALRRVANEARHVQR
                     HLTDDDVSLVRALLVHVLRWHFSAFPRGPRLPSLWAHDSPELDPLALFVDSLELGEAI
                     QQKWDADPRFARSSLTSALYIETLIADGSEDARIVAERVITDALTRHPKDLRLRQLDG
                     WLARLRGDLDASAQKLEAVLDESEDVETVGLLAGTYKRMGKLQRSAELYRRAWKQSER
                     RSTYAGINAASMSLLAGQRDVASQIAMQVLGLFERRNTQGGALEFTGYWELATYAEAK
                     LLTGDVDGARALYRRVFSTYASQRGWIASTRSQLEQLLRVLGGGNVETFLTAV"
     gene            complement(5512..7026)
                     /gene="ligD"
                     /locus_tag="VMZ53_14900"
     CDS             complement(5512..7026)
                     /gene="ligD"
                     /locus_tag="VMZ53_14900"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HUS29790.1"
                     /translation="MGLDRRRGGRRKRRPSGESAFSFARQITLAPITSGTAVAVSEHL
                     IEPAWDGHRVLATRVGDDVRIAALDFRDWTQTFPAAARALAKLTAQKLAIDGVVCVLD
                     ERGVPSFDKLREAVAAGAAVTDAVVICWDLLWDGDEDLRTKPLTERRKRLTALLGNAP
                     RTLMHSEALQGSLARVLEAAAPLGLRGVVARPLGGDYTAPWCAFSTSSAAIDFRRSLS
                     PPPPLSNADKVLYPRDGICKRDIVAYYRDVAPLLLPHLLDRPVVIQRWPDGIDEFDWY
                     QHRVPPRAPDYIRAAYVDGVRRIVIENADALLWMVNQAGLTYHGFLSRLSTLAEPDVA
                     MIDLDPGDKTTWPDIVEIALAVRRLLELLELPSVLKTSGQRGLHILIPLAPGHTFAQA
                     EELCRGVARMLLRLMPDKITDEQEKKERGGKLLVDTKQFMAKTLVVPYSLRAADGATL
                     STPLAWDELTPKLDPRQFTLRSVRSRLDAKGDVAAPLLRGTTKLEAALTLLRRQ"
     gene            complement(7017..7838)
                     /locus_tag="VMZ53_14905"
     CDS             complement(7017..7838)
                     /locus_tag="VMZ53_14905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HUS29791.1"
                     /translation="MAYRLLAVDLDGTLLRADKSIDPTDIAAIRELAGAGVTVTIVTG
                     RLHSGSVEAARACGIAGAIACVEGSHIVDAESELTHHRHGMAPDVTRVLRTTFGTHGL
                     ASFIFEHDGIHHDRAGEPYAGYVSTWSPRMRMVEEALDERVWSNDPLAAVAIGSEAEV
                     AAAHAELKAHATNIFSVSFAVAWVPGKYALLVRAAGPTKGTALRQLCERQGITTADAV
                     VVGDWVNDVPMFEVAGRAFVMGGAPDAVRSKATDTLERQTGDGGGIAEAIRRAWG"
     gene            7888..8700
                     /locus_tag="VMZ53_14910"
     CDS             7888..8700
                     /locus_tag="VMZ53_14910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29792.1"
                     /translation="MPDLSGVVSRIAAAGRAAWPDVRLSEDIIARNLSDRVKQDPETQ
                     LENLHDADLYLAMALAEADQAALRAFETDLVPQMDIALRRLRLAGGTAEEVKQAIRFE
                     LLVGEGRKKISDYAGRGELAAWLRVTATRKALKAVRRLDREETLDEILLDHWPDATPG
                     PEKKQLRGQYTAELKKAIREAFGALEVRQRNLLRQHILDELTIDDLARLYRVHRATCA
                     RWLADARADLGKHTRKKLTQALGLGNKDEMDSMLRFLDSDIELSISRILMSR"
     gene            8697..10793
                     /locus_tag="VMZ53_14915"
     CDS             8697..10793
                     /locus_tag="VMZ53_14915"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29793.1"
                     /translation="MSLCPAPDQLLGFASGELSDDQSASLEQHLDSCAECRAVLSNMA
                     RGGPPPSFGRYRIDTVLGSGGMGIVYRAYDPQLARSLAIKVVRRAGDDTQGRARLVRE
                     AQALARLSHPNVCHVYDVGTEGEEVWLAMELIDGVQLRQWAGERRQREEILSVLLGAA
                     EGIAAAHNAGLVHRDIKPENVLVTRDNRAIVTDFGLARAEDAVNPNASTISADPHLTA
                     TGAIAGTPAYLAPEQLLGDPIDQRVDQFAWAVMAWELLTGSRPFPIVFAVRVEAIRAG
                     VKAPPELPRGLAAPLMRAMAASPSDRYASMRELIDALKQPGTATLKTKRRDRKSAVPV
                     IAAAVGLLALGGGATAIALMNRDDKKQVAAAQPAPPPPIDPVVQPQPQPAPPDPAPVQ
                     QPQVATPAPQPVQTPKPVTVAKVTKKDPKGVPVQPTATQPAQPAQPTAQQQPAAPPKP
                     QGTIGLTPQTSADPSAWADSQGHKYCRSCQVATADSFCFIPYDFDKPSGKGRHGIVDW
                     GTVTKVDDEIGSFRDEQFKETVITMRGQRKTYRFTADLMLGRLVTKVGALLAVCEEDE
                     SDFYQFNGGPTGRMQAVITLSAPPAIALAAKNRAQHVNELTFARVSLNADLENLDPKG
                     SYLVYVRPESIDGPEWRMDRYWVDVPAGTPGEKLLAPKKMVWMIAKNPEIYDRPDGKK
                     GLRVHAVAFFDELFPD"
     gene            complement(10794..11693)
                     /locus_tag="VMZ53_14920"
     CDS             complement(10794..11693)
                     /locus_tag="VMZ53_14920"
                     /inference="COORDINATES: protein motif:HMM:NF007695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUS29794.1"
                     /translation="MLPRFAHGPETITLGDLEVRRLGYGAMRLPGKQVWGEPDDPATA
                     HAVLRRAIELGINFIDTAWYYGPLVANRLIVESLHPYPRDLVIATKLGAKRLEDKSWA
                     PALTVDELREGVHTDLRTLKLEQLPLVHLRWTERAQISFGEALDVLIDMQKAGKIRHL
                     ALSNVTVEQLEAATKKTAIVAVQNMFNVSGTAGPGPAAGNPEAVLAKCEEKGIAFVPF
                     FPLGAGAGGSSTVEQVAKRLNTSASQISIAWLLARSPQMLPIPGTSKISHLEENVGAV
                     HVALDDATLAELSARKSDPQIRS"
     gene            complement(11729..12493)
                     /locus_tag="VMZ53_14925"
     CDS             complement(11729..12493)
                     /locus_tag="VMZ53_14925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS29795.1"
                     /translation="MPRPVSLSSTFADHFSSVAQRYAQYRPHYPQALADALAALCSRR
                     DAAWDAGAGNGQLSVQLAKRFAKVYAQDPSQAQLDAALPFPNVEYRCAKAEESGLPDG
                     SVDLAVAAQAAHWFDWPRYVAEVERIARPGALVALVSYGIMHVGGEADAIVARYYHDD
                     VGAYWPKGREHVENGYRDLVWPWPAVEAPAIDMSVDWTRDELCGYLATWSATAKRIEA
                     KGPAAYEAVCSRLASVWPDGERRTVRWPLAIRLARR"
     gene            complement(12472..13080)
                     /locus_tag="VMZ53_14930"
     CDS             complement(12472..13080)
                     /locus_tag="VMZ53_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29796.1"
                     /translation="MVRGGALALLLGMAVATPAWADEPVSPVTFHKGQFGGSVRFGVG
                     ARGIATYENSVYCGKTDPQAEYGFASVCTGRTPLALDLEAAYGVTGAIELTLELRIGI
                     ESDFGTTPSEDGPRPFHVAPGARFFFSEAARSKLFVQPELVFDFAGYDRGNDFGVRGI
                     EGYWIDLHKTYGLYFFVAETLEFSRWLSASFEAGVGFQGRYP"
     gene            13115..13906
                     /locus_tag="VMZ53_14935"
     CDS             13115..13906
                     /locus_tag="VMZ53_14935"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HUS29797.1"
                     /translation="MLTRRQLLGGAALGLLATRFRTAHAIGPSSKFRFGQLQLGAAWN
                     PRPNALRRLGWEVEKQTSIDVELDPAIVTPTSETLHETPFLYFAGEKAVDLPTSAGIE
                     ALRRFLTFGGFLLIDNAEGSTDGAFDGSVRKLMQAIFPAPNKGLEIVPTDHVVYKSFF
                     LLDKPVGRLAIAPTMEGIFYDKRLVCVYVANDLGGAWARDDFGNYDFPCEPGGEKQRD
                     LAFRLGINLVQYALCLDYKSDQVHVPFIMKRRRWKPDDGASSPTK"
BASE COUNT         2489 a         4527 c         4589 g         2351 t
ORIGIN      
        1 caacttgccg ccagccggcg gtgcgccgcg tgcgagcgcc gagtccggcg ggaccgcgac
       61 cgaagtcgac acggagacgg acacgacaga gacgctgaag ctcgaggcat ttgccatcac
      121 accgaacggc acgtacgcgg ccgcgaaggt tggcaaaaca ctccacgtct ggaacgtgtc
      181 cagcggggac caagtcggag ttgcggagct gggatgccag gttgccatcg atccgagcgg
      241 catcgacctc gatacgccct gcaacgagcg caacctgggc tcggtcatcg atcttcagat
      301 cagtcccgat ggcgcctgga tcatcgtggt cggacaaagc gccgcagagc tctttcatcg
      361 caccaaagac gctcttgttc ccgcgccggt accagccggt ggtaccgccc attgccggct
      421 caagcccgac gacgagctct atagctatga gcgctctcgc cttggattct ctgccgcgtt
      481 ctccgggaat agctcggtac tcgcggttgg acgtggccgt tccatcgaca cgtggcgcct
      541 cgacggcacc gcaccaactc gcatcgagac gatctgtgca aacgcggaat cgctagcgct
      601 cgatttcggc gggacgtggc ttgcgcgtcg taatgatatc gagcgcagcc gttgttacta
      661 cgagacaacg aagccgtgcg ggttctttct gtatgtctta cgcgacgacc gatggattgg
      721 cttctcgagc cagcacattc gagccttcga ttcgcaccaa gcgggcgtcg cactcgagcc
      781 cagcaagata gggtttagcc gtacggatct cgcggcgcat tcgagctcgc ccgttcgaac
      841 gagctgcgac tacgacagct acttcctatc gcaaggcacc gccgtctcgg cctcttcgtg
      901 cgggtacgtt gtcgacgacg gcaagtccgt cattgcaggc gtgcttccac tcggcactac
      961 gctcgccgtc gccccgctag cgcgtgtcgt cgccgatgcc gacgatggca cggtacggat
     1021 ctggaagctg agtcgttcgc tcgagaatgt cgtgcagacc actgcggatg ccaccccgct
     1081 tgcgaagacg agcattgcac ccggcggtgt ctggagcggc acgattgcag tagcccggga
     1141 cggactgacc ctcgcgatcg gaagcagcga tacagcgtcc gattctcgag aacagatagt
     1201 cgatgggaag cctccggtcc tggcgctgtt ctcgacggtc gagagcgccg aaaaaacgga
     1261 gctcgtccgc acgtccggcg agatgccgca attgctcgac aagaacggca accacggcgt
     1321 ggcatacgtc gatttcgatc ctgcgcaaga gcgcgtttac tcgctcggac gcgacggcca
     1381 cgtccggatc tggcggcgaa gcgatggctc gaagctcctc gacctcggca acgagcaggg
     1441 ccgtatcctg gccgctgggt tcacgcccga cggctcgaag atcgcgacgg gtgatcacgt
     1501 cggccggctt tgcgcgtgga acagcgcgac gggtcaaaag ctcgcctgta gcgagccgac
     1561 gaacgtcgcg ctccgtcgcg gcgcattcgc gatgacaccc gccggagcct ttatcactac
     1621 ggacgtgaag ggaacgatca gactctggaa gctcggagcc gaagcgccca tcgtcgtcgg
     1681 caaccacgcg ctggccgtcg atcggctcga agtcgacaag actggaaaat ggctagcgag
     1741 tgcaagcaac gatggaaccg cgcgcttgtg ggatctgacg acctgtgcgc ccagcggatg
     1801 caaggagagc cttcgtctcg aggatccatc ggggttcgat gatgtcctgc ttccgagcga
     1861 taacggcacg acgtggctca tcgcgactcc acatgacgat cgagtcgtcg tcgtggacct
     1921 cgcgaaaccg gccgagcgcc atgttctgac aacgcgcaac tgggccagct ccgtcgacgt
     1981 gagtcgtccg ggacacctgc tcttgtgtga gttcggcggc accgtcgaag aatgggacct
     2041 ccgtacgaag cggttgtcgg gccgcttgct gccaagcgac gaccgcgcct ggtgcggtgc
     2101 gaagtacgtc ggcgaaaatc ggttcatcac gcaattcacg aacggcgagg tagccctgtg
     2161 gagtatcgac gctctcgcgc gtggcttgac cggatggtcg acgatgggag gcctgtcctt
     2221 cgatgcgagc ggcgcaatcg tcggcacgcc gcgactctcg agtggtgcac gcgacgtact
     2281 cgtcatgtcg cgggatggca acgtcacagc acgtgtgcca gcggtttcgc gcgcggttgt
     2341 cgacgacaag gccatcatct tcggaactcc caatggaggc attggcgtcg tcgacgctgc
     2401 cggcaatgga gcgttgtcga gtggaaccga ttacgtcgcg agcacgattg cggcgtcaca
     2461 tggagagcgg ctcgtcgtca cgtgggatct ccactacgag ctcgcgccga gcgcgtcggt
     2521 ccaagcgcct gtctggcgtg cgctgccatc gagtcttccg catggatccg tccgtgatgc
     2581 tcggcttgcc gctggctcct ggatcgtggc tgccgcgcaa gacgattcgg tgacccgcta
     2641 cgacgccaca ggacaccgaa acgacacgag cacactcaaa ggtgccgctc atgtagtcga
     2701 ggcggataac ggcgacggct tgctggtggt cggagatttc gatgttgcga ccatcggcgc
     2761 aaccggacct ccggtggtgc acaagatgat gtcaggcgtc tactcgcggg cagcggcatg
     2821 ggacgtcggg gcctcgttgc tcgcactcgc atcgagaaac aatgttgtcg agattcgcga
     2881 tctctccacg aacaaggtcc tgtggagtga gagcaagaca ttcggaaccg acaagtacta
     2941 cgcgcaactg atcaagttcg gcacggtggg aacgcagcga gtgatcgcgc tcgcgacgga
     3001 aggctatatc gggatctacg ccgtcgcgac gggcaagctc gtccaggagt tcctcgagaa
     3061 gacaaagcag aagggcgacg ttctagacct ctggttcctc gaagacggca acttgctcac
     3121 gcatgacgat gcagcacgcc tagtgctctg ggattcccgc acggggaagc agctcgagat
     3181 gctgcaggac atcaatgacg ttgtactggg ccccggaaat cgcttcctcg tgacgcggtg
     3241 gggcgagatg cgctacgtga cgtacaagaa caagaagctc gacctgtcgg atccgcatct
     3301 cgactgcgga cgctacgtgt atcgaatcgc gtcatcggcg accggcaaac ttttcgcggc
     3361 cgcttcccaa cccaagaacg agaccgatag cgcgcagctc tgcctcatcg atgccgccac
     3421 cggacgagtg gtcacgacgt ggtcatcaaa gcagacgcag atcacgcgcc ttgcgtttcg
     3481 tcgcgacgac accgcgctgc tgagcattgc ggacgagccg gcgccggtcc tgtgggaagt
     3541 cccaacgggc aaacagctac aggtattcga gcacgaagac ttgctcgtgg cgggctttgc
     3601 caaaggcacg ccgtacatcg tgacggctga taccagcggc aagcttcgaa tctacgacgc
     3661 gcgcgcgggc cgaatggtcg cggagtcgac gcaacccccc ggagcggtcc aggtggtcag
     3721 ctcgggtttg atcgacgaga cctccggaat tctcacgacc accgatgctc gcggtgtgat
     3781 cgtccagatc gatctcgcga ctggcaggcg tctgcgcgag ttctcgacgc agcgccgaga
     3841 gatccgagcg atcgcggttg cgccgggtgg aagcacgatc gcggcagccg tcggcagcga
     3901 ggtccagctg tggagaaacg cgcctgggct gctcgttccg gtcgcgctcc cgccgatcac
     3961 gctcgatacg ggcgaggtcg atggcttgag cttctcgcca gatggccgtt ggttggcagc
     4021 ggctgatgga ttcagcgtgg agaacaccga agcggagcac ggtttgttga cgctctggga
     4081 tgcaagtgac ggcagcaacc tgctgcgcaa ggacacgccg gttcggctta cctcggtcgc
     4141 atggtcaaac gacgcccgcg aggtcgtcgt cgcgggcaac gccggatatc tgcaagtggt
     4201 ctcgtttggc ctcgagcagc gtcccgtcga ggtcgtgcgc aagattctcg aacgcatggc
     4261 acctcggacc acgtaccacc cgactcagtc gccgcgaaaa ccgagcacca gcggcggtgg
     4321 aatgatcgat atctagacgg cagtgagaaa cgtctcgacg ttcccgcctc cgagcacgcg
     4381 caacagctgc tcgagctgcg agcgagtcga cgcaatccag ccccgctgcg acgcatacgt
     4441 cgagaacaca cgccggtaca gcgctcgcgc accgtcgacg tcgccggtga gcagctttgc
     4501 ttccgcgtac gtcgcgagct cccaataacc ggtgaattcg agggcaccac cttgggtgtt
     4561 gcggcgctcg aacaagccga gaacctgcat agcaatctgc gacgcaacgt ctcgctgtcc
     4621 tgccaacagc gacatgctcg ccgcgttgat accggcgtac gtgcttcgcc gctcgctctg
     4681 cttccacgcg cgtcggtaca gctcggcgct tcgttggagc tttcccatgc gcttgtacgt
     4741 accggcgaga agacccaccg tctcgacgtc ctcgctctcg tcgagtaccg cttcgagctt
     4801 ttgtgcggaa gcgtcgaggt cgccgcgcaa gcgcgccagc caaccgtcga gctgccgcag
     4861 ccgcaggtcc ttcggatgac gggtcagagc gtccgtgatt acgcgctcgg cgacgatgcg
     4921 cgcatcctcg gacccatcgg cgatcaacgt ttcgatgtag agcgcgctcg tcaacgatga
     4981 ccgcgcgaaa cgcgggtccg cgtcccactt ctgttggatt gcttcgccga gctcgagact
     5041 gtcgacgaac agcgcgaggg gatcgagctc gggtgagtcg tgggcccaga gcgagggcaa
     5101 ccgtgggcca cgagggaatg cgctgaagtg ccaccgcagt acgtgcacca gcagcgcgcg
     5161 aacgagcgag acgtcgtcgt cggtcaagtg gcgttgcacg tgacgcgctt cattcgctac
     5221 gcgccgcaat gcatgcgcga gccgcgccgg gaattcgggg atcacgccga gctcggagag
     5281 ccggacgaga ttgccgtaca cattgttgga gggctctccg ccgagcgctc gcacttcttc
     5341 ggcgattagg acctccaaaa tgcgtcctgc atagaacaca gcagcctcgg gagcatcgac
     5401 acccgcgacg cgctcgagtc ggtcgagatc ggcgccgagc gttgtgccgg agtattcggt
     5461 ggcgcgaact tcggccgcga ggtcactggc gcgcatcgat gaccattcta gctattgtcg
     5521 cctcaggagc gtgagcgccg cttcgagctt cgtggtgccg cgcaggagcg gcgcggcgac
     5581 gtcgcccttt gcatcgaggc gcgagcgaac gctgcgcagc gtgaattgcc gaggatcgag
     5641 ctttggcgtt agctcgtccc aggcgagcgg cgtcgagagc gtcgcgccgt cggccgcgcg
     5701 cagcgagtag gggacgacga gcgtcttcgc catgaactgc ttggtgtcga cgagaagctt
     5761 accgccgcgc tctttcttct cctgctcgtc ggtgatcttg tcgggcatga ggcggagaag
     5821 catgcgagcg acgccgcggc agagctcctc ggcttgcgcg aacgtgtgac cgggcgcgag
     5881 cgggatgagg atgtggaggc cgcgctggcc gctcgtcttg aggacggacg ggagctcgag
     5941 cagctcgagg aggcgacgga cggcgagcgc gatctcgacg atgtcgggcc acgttgtctt
     6001 gtcgccggga tcgagatcga tcatcgcgac gtctggctcg gcgagcgtcg agaggcgcga
     6061 gaggaagccg tgatacgtga gcccggcttg gttgaccatc cagaggagcg catcggcgtt
     6121 ctcgatcacg atgcggcgaa ctccgtcgac gtatgcggcg cggatgtagt ccggcgcgcg
     6181 cggtgggacg cggtgttggt accagtcgaa ctcgtcgatg ccgtcgggcc agcgttggat
     6241 cacgaccggg cgatcgagga gatgcgggag gagaagcgga gcgacgtccc ggtaataggc
     6301 gacgatgtcg cgcttgcaga tgccgtcgcg cggatagaga actttgtcgg cattcgagag
     6361 cggtggcggt ggcgagaggc tgcggcggaa gtcgatcgca gcggagctcg tcgagaatgc
     6421 gcaccacggc gcggtgtaat cgccgccgag cggacgcgcg acgacgccgc gaaggccgag
     6481 tggagcggct gcttcgagca cgcgcgcgag cgagccttgc agagcttcgg agtgcatcag
     6541 cgtgcgaggt gcgttgccga gcagcgcggt gagccgcttg cggcgctcgg tgagcggctt
     6601 tgtgcggagg tcttcgtcgc cgtcccagag gaggtcccag cagatgacaa ctgcatccgt
     6661 cacggcagcg ccggccgcga ctgcttcgcg cagcttgtcg aacgatggga cgccgcgctc
     6721 gtcgagcacg cagacgacac cgtcgatcgc gagcttctgc gcggtgagct tggcgagagc
     6781 gcgtgcggcg gccgggaagg tctgcgtcca gtccctgaaa tcgagtgccg cgatgcgaac
     6841 gtcgtcgccg acgcgcgttg cgagcacgcg atggccgtcc cacgctggct cgatcaaatg
     6901 ctcggagacc gcgaccgcgg tgcccgacgt gatcggcgcg agcgtgatct ggcgcgcgaa
     6961 cgagaatgca ctctcgccac tcggacggcg cttgcgacga ccacctcgtc gccgatctag
     7021 ccccatgcgc gtcgaatggc ttctgcgatc ccgccgccgt cgcccgtctg ccgctcgagc
     7081 gtatcggtcg ctttgctgcg aactgcgtct ggcgcaccgc ccatcacgaa cgcgcggccc
     7141 gcgacctcga acatcggcac gtcgttgacc caatcgccga cgacgactgc atctgcggtg
     7201 gtgatgcctt ggcgctcgca gagctggcga agtgcggtgc ctttcgtcgg gcctgccgcg
     7261 cgcacgagca gcgcgtattt gcccggaacc catgcgaccg cgaagctgac cgagaagatg
     7321 ttcgtcgcgt gcgccttcag ctcggcgtgc gcggcggcga cttccgcttc actgccgatc
     7381 gcgaccgcgg cgagtggatc gttggaccac acgcgttcgt cgagcgcttc ctcgaccatg
     7441 cgcatgcgcg gcgaccacgt cgagacgtag ccagcgtatg gctcccctgc ccgatcgtgg
     7501 tggatcccgt catgctcgaa gatgaagctc gctagaccgt gcgtaccgaa cgtggtccgc
     7561 aatacgcgag tcacgtcggg tgccatgccg tgccggtggt gggtcagctc ggattccgcg
     7621 tcgacgatat ggctgccttc gacgcatgca atcgcgcctg cgatcccgca agctcgcgcc
     7681 gcctcgacgg atcccgaatg cagccggcct gtaacgatgg tgaccgtgac accggccccc
     7741 gcaagctcgc ggatcgccgc gatatctgtg gggtcgatcg acttgtcggc gcgcagcaag
     7801 gtgccgtcga ggtccacggc caacagccga tacgccatgg ccgattctac cagtggcagt
     7861 cgaccggcgg gatagtagga tgagcgcgtg ccggacctga gtggcgttgt ctcccgcatt
     7921 gcggcggccg gccgtgctgc gtggcccgac gtccgcctat ccgaggacat catcgctcgg
     7981 aatctctcgg atcgcgtgaa gcaggatccc gagacgcagc tcgagaacct gcacgacgcc
     8041 gacctctatc tcgcgatggc gcttgccgaa gcagaccaag cggcgctgcg tgcgttcgag
     8101 accgacctcg ttccgcagat ggacatcgcg ctgcgccggc ttcggctcgc gggtggaacc
     8161 gcagaagaag tgaagcaggc gatccgcttc gagctcctcg tcggcgaggg ccgcaagaag
     8221 atttccgact acgcgggccg cggcgagctc gctgcatggc tacgcgtgac cgcgacgcgc
     8281 aaggcgctca aagcggtgcg ccgtctcgat cgcgaagaga cgctcgacga gatcctgctc
     8341 gaccactggc cggatgcgac gccaggcccc gagaagaagc agctgcgtgg ccagtacacg
     8401 gccgagctga aaaaagcgat ccgcgaagca ttcggcgcgc tcgaagtacg ccagcgcaat
     8461 ctgctgcgcc aacacattct cgacgagctg acgatcgatg atctcgcgcg cctctaccgc
     8521 gtgcatcgcg cgacgtgtgc acggtggctc gccgacgcgc gcgccgatct cggcaagcac
     8581 acgcgcaaga agctcacgca agcactcggc ctcggcaaca aggacgagat ggacagcatg
     8641 ctgcggttcc tcgacagcga tatcgagctc tcgatctcgc gcattctgat gtcgcgatga
     8701 gcctctgccc tgctcccgat caactgctcg gcttcgcctc cggagagctg tccgacgatc
     8761 aatcggcgtc gctcgagcag cacctcgaca gctgcgcgga gtgtcgcgcg gtgctctcga
     8821 acatggcgcg tggtggtcca ccgccgagct ttggccgcta tcgcatcgac acggtgctcg
     8881 gctcgggcgg catgggcatt gtctatcgcg cgtacgatcc gcagctcgcg cgctcactcg
     8941 cgatcaaagt ggtgcgccgc gcgggcgacg acacgcaagg tcgtgcgcgc ctcgttcgcg
     9001 aagcgcaagc tctcgcgcgg ctctcgcatc cgaacgtctg ccacgtctac gacgtcggca
     9061 ccgaaggcga agaagtctgg ctcgcgatgg agctcatcga cggcgtgcag ctgcgccagt
     9121 gggccggcga gcgacgtcag cgcgaagaaa tcctctcggt gctcctcggt gcggccgaag
     9181 gaatcgccgc tgcgcacaac gccgggctcg tgcatcgcga catcaagccg gagaacgtgc
     9241 tcgtcacgcg cgacaaccgc gcgatcgtca ccgacttcgg tctcgcgcgc gccgaggacg
     9301 cggtgaaccc gaacgcgtcg acgatctccg ccgatccgca tctcaccgcg accggcgcga
     9361 tcgccgggac ccccgcgtat ctcgcgcccg agcagctcct cggcgatccg atcgatcagc
     9421 gcgtcgatca attcgcgtgg gcggtcatgg cgtgggagct gctcaccgga agtcgcccgt
     9481 tcccgatcgt cttcgcggtg cgcgtcgaag cgattcgcgc gggcgtgaag gcgccgcccg
     9541 agttgccgcg cgggctcgcg gcgccgctga tgcgcgcgat ggcagcatcg ccttccgatc
     9601 gctacgcgtc gatgcgcgag ctgatcgatg cactgaagca gcccggtacc gcgacgctca
     9661 agacgaagcg ccgcgaccgc aagagcgcag ttcctgtcat cgccgctgcc gtcggcctgc
     9721 tcgcgctcgg cggaggtgcc acggcaatcg cgctgatgaa tcgcgacgac aagaagcaag
     9781 tcgccgccgc gcagcctgct ccaccgccgc cgatcgatcc ggtcgtgcag ccgcagccgc
     9841 agcccgcacc gcctgatccc gcgccggtgc aacagccgca ggtcgccact ccagcgccgc
     9901 agccggtgca gacgccgaag ccggtcacgg ttgcgaaggt gacgaagaaa gacccgaagg
     9961 gcgtcccggt gcagccaacg gcgacgcaac cggcgcagcc cgcgcaacca acggcgcaac
    10021 agcagcccgc cgcgccgccg aaaccgcagg gcacgatcgg tctcacgccg cagacatcgg
    10081 ccgatccgag cgcgtgggcg gactcccagg gacacaagta ttgccgcagc tgccaggtcg
    10141 cgacggccga ctcgttctgc ttcatcccgt acgacttcga caagccgagc ggcaagggcc
    10201 gtcacggcat cgtcgattgg ggcaccgtca cgaaagtcga cgacgaaatc ggatcgtttc
    10261 gcgacgagca gttcaaagaa acggtcatca cgatgcgcgg ccagcgcaag acgtaccgct
    10321 tcaccgcaga cctcatgctc ggccgcctcg tgacgaaagt cggtgcgctg ctcgcagtgt
    10381 gcgaggaaga cgagagcgac ttctaccagt tcaacggcgg gccgacgggc cgcatgcaag
    10441 ccgtcatcac gctctccgca ccacctgcga tcgcgctcgc cgcgaagaat cgcgcgcagc
    10501 atgtcaacga gctgacgttc gctcgcgtca gcctcaacgc cgacctcgag aatctcgatc
    10561 ccaaaggttc atatctcgtc tacgtgcggc ccgagtcgat cgacggcccc gaatggcgca
    10621 tggatcgcta ctgggtcgat gtccctgccg gtacgcccgg cgagaagctg ctcgcgccga
    10681 agaagatggt ctggatgatc gcgaagaacc cagagatcta cgatcgcccc gacggcaaga
    10741 agggcctgcg cgtccacgcc gtcgcgttct tcgacgagct gttcccggac tgactaagac
    10801 cggatctgcg gatcggactt gcgtgccgac agctcggcaa gcgtcgcgtc gtcgagcgcc
    10861 acgtgcaccg caccgacatt ttcttcgaga tgcgaaatct tgctcgtgcc gggaatcggc
    10921 agcatctgcg gcgagcgcgc gagcaaccac gcgatcgaga tttgcgacgc cgacgtgttg
    10981 agccgcttcg cgacctgctc gaccgtgctc gacccgccag cgcccgcacc gagcggaaag
    11041 aacggcacga acgcgatgcc cttctcttcg cacttcgcga ggacggcttc gggatttcct
    11101 gccgctggac cggggccggc tgtgccactc acgttgaaca tgttctgcac cgctacgatc
    11161 gcggtcttct tcgtcgcagc ttcgagctgc tcgaccgtta cgttgctgag cgcgaggtgg
    11221 cgaatcttgc cggccttctg catatcgatg agaacgtcga gcgcctcacc gaacgagatc
    11281 tgcgctctct cggtccaacg caggtgtacg agaggaagct gttcgagctt cagcgttcgc
    11341 agatcggtgt ggacgccttc gcgcagctcg tcgaccgtga gcgcgggagc ccagctcttg
    11401 tcttcgaggc gcttcgcgcc gagcttcgtc gcgatcacga ggtcgcgcgg atatggatgg
    11461 agcgattcga cgatcaggcg attcgcgacg agcgggccgt agtaccaggc ggtatcgatg
    11521 aagttgatcc cgagctcgat cgcgcgccgc aggacggcgt gtgcagtcgc cggatcgtcg
    11581 ggctcgcccc acacttgttt gccgggcagg cgcatcgcgc cgtagccgag gcgacgcacc
    11641 tcgaggtcgc cgagcgtgat cgtttcgggg ccatgagcga agcgcggcag catctaccgc
    11701 acacgatgcc atcgcccgcg gatggtgcct agcgacgcgc gagccggatc gcgagaggcc
    11761 atctaactgt gcggcgttcg ccatcgggcc agaccgacgc gagtcgggag cagaccgctt
    11821 cgtacgcggc gggccctttc gcctcgatgc gctttgccgt cgcagaccac gtcgcgaggt
    11881 agccgcacag ctcgtcgcgc gtccagtcaa cgctcatgtc gatcgcgggc gcctcgacgg
    11941 cgggccatgg ccacacgaga tcgcggtagc cgttctcgac gtgctcgcgg cctttcggcc
    12001 agtacgcgcc gacatcgtcg tggtagtagc gcgcgacgat cgcgtctgct tcgccgccca
    12061 cgtgcatgat gccgtagctc acgagcgcga cgagtgcgcc cggccgcgcg atgcgctcga
    12121 cctccgcgac gtagcgaggc cagtcgaacc agtgtgcagc ctgtgcggcg accgccagat
    12181 cgacgctgcc gtcgggcagg cctgactctt cggctttcgc gcaccggtat tcgacgttgg
    12241 gaaacggcag cgccgcgtcg agctgtgctt gcgacggatc ttgggcgtag accttcgcga
    12301 agcgctttgc gagctgcacg gagagctggc cgttgccggc acccgcgtcc cacgcagcgt
    12361 cgcgtcgcga gcacagcgcg gcgagcgcat cggcgagcgc ttgtggatag tgcgggcggt
    12421 actgcgcgta gcgctgcgcg accgaggaaa aatgatctgc gaacgtgctg ctcagggata
    12481 ccggccttgg aatccgacgc cagcttcaaa cgatgcggac aaccaccgcg agaactcgag
    12541 ggtctcggcg acgaagaagt agaggccgta cgttttgtgc aggtcgatcc aatagccttc
    12601 gatgccgcgc acgccgaagt cgttgccgcg gtcgtacccc gcgaagtcga agacgagctc
    12661 gggctgcacg aacagcttgc tgcgcgccgc ctcgctgaag aagaaccgcg cgcctggcgc
    12721 gacgtggaat ggacgcggtc cgtcctcgga cggggtcgtc ccgaaatcgc tctcgatacc
    12781 gatacgcagc tcgagcgtca gctcgatagc gccggtgacg ccgtaggctg cttcgaggtc
    12841 gagcgcgagc ggcgtgcggc ccgtgcagac cgatgcaaag ccgtattccg cctgcggatc
    12901 tgtcttgccg cagtacacgc tgttctcgta ggtcgcgatg ccgcgcgcgc ctacgccgaa
    12961 ccgcacggac ccgccgaact ggcccttgtg gaacgtcacc ggggacaccg gctcatccgc
    13021 ccacgcaggc gtcgcgaccg ccatcccaag caggagcgcc agagcaccac ctcgcaccat
    13081 tgcggcgaac ctatcacgac gctaggttct cggaatgctt acccggcggc agctgctcgg
    13141 tggtgctgcg ctcggcctcc tggccacgcg ctttcgcact gcgcacgcga tcgggccgag
    13201 ctcgaagttc cgcttcggtc agctccagct cggcgccgcg tggaacccgc gacccaatgc
    13261 gctgcggcgg cttggctggg aggtcgagaa gcagacgtcg atcgacgtcg agctcgatcc
    13321 ggcgatcgtg acgccgacga gcgagacgct gcacgagacg ccgtttctct acttcgccgg
    13381 cgagaaggcc gtcgaccttc cgacgtcggc aggcatcgag gcgttgcggc gcttcctcac
    13441 cttcggcggg ttcctcctca tcgacaacgc ggaaggctcg accgacggcg cgttcgacgg
    13501 cagcgtccgc aagctgatgc aggcgatctt tcccgcgccg aacaaagggc tcgaaatcgt
    13561 cccgaccgat cacgtcgtct acaaatcgtt cttcttgctc gacaagcctg tcggtcgcct
    13621 cgcgatcgca ccgacgatgg aaggcatctt ctacgacaag cggctcgtct gcgtgtacgt
    13681 cgccaatgat ctcggcggcg cgtgggcccg cgacgatttc ggcaactacg atttcccgtg
    13741 cgagcccggc ggcgagaagc agcgcgacct cgcgttccgg ctcggcatca acctcgtgca
    13801 gtacgcgctg tgtctcgact acaagtcgga tcaggtccac gtgcccttca tcatgaagcg
    13861 gcggcgctgg aagcccgacg acggcgcgag tagcccgacc aaatgaacat cgtcctgttt
    13921 tcctcgacgc tgcggctagg gctcgcggtc gcagga
//
LOCUS       DATLBZ010000286         5695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2550938, whole genome shotgun sequence.
ACCESSION   DATLBZ010000286 DATLBZ010000000
VERSION     DATLBZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550938"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /gene="queG"
                     /locus_tag="VMZ53_14940"
     CDS             <1..853
                     /gene="queG"
                     /locus_tag="VMZ53_14940"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HUS29798.1"
                     /translation="RSLIVVALAYDRVDPVVSLDRLRGRIARYARGEDYHLVMRDKLV
                     LLADRLSAALGRPVASRPCVDSAPVLEREWAERGGLGFVAKNTMVIAPGLGSYIVLGE
                     LLVDVELATTAPAEPAKPRCGSCRACLDACPTGAFVDAYVLDARKCISYLTIENEGPI
                     PVELRPAIGTWVFGCDICQEVCPFNAGAGDAPDPMLRPRTGEHALPDLVALATKNTNQ
                     LRQFVKRTALRRVSRENLLRNVAVALGNTGSAAAIPALEHLLQNKSELVREHAQWALD
                     HLRAMAG"
     gene            965..1471
                     /locus_tag="VMZ53_14945"
     CDS             965..1471
                     /locus_tag="VMZ53_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29799.1"
                     /translation="MATLTRKQFLQSALGACAGLFGLSMLSSCNDQTMPRGDGGVGAD
                     SVGGAGGGGGGGGGGGGGGETVDAGGGGGGGGGNETVDGGTTNRCLANGTVSTIGTNH
                     GHVLVVPKEDVAAGTAKTYNIQGTSGHPHTVTIRSEHFAQLAEEEAIMTVSSSDAGHS
                     HAVMVACA"
     gene            complement(1475..1855)
                     /locus_tag="VMZ53_14950"
     CDS             complement(1475..1855)
                     /locus_tag="VMZ53_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUS29800.1"
                     /translation="MASKHSTWHVVTVKLRYALGLVATVALSGCEQKLAGGSADGAKV
                     FQSICAACHGPKGKPSAQAVATTGVKDLTSQELRHRITPALVENQVRKGSQNKLMPAF
                     EGALTDEQIKAVAAYVASPEFLDQ"
     gene            1854..2741
                     /locus_tag="VMZ53_14955"
     CDS             1854..2741
                     /locus_tag="VMZ53_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29801.1"
                     /translation="MGRASVLLLLIVAACGGAQIPQHNGYKAKAKPWKKAKTLKFDDK
                     SEAKATGELSYAEFKRAAWFVADLQQPGQLDIKLDITPPGEEVDEDFDLGFEVLDAGN
                     RVIARSNKEEGDDTGEMQKSKSLLDLEPGRYMIHLYLQDRRDTCDYVMRATFKPMSSV
                     GKSDFPSQVAFTPSLPMVPLQDDTPKGYKSPTTTTTVVVKKPRTGGGQKPPPPPPPPP
                     PTTKSARIIGMQVVGGGTQITIGLGTAGGASVGMKGKINNVASGGFTLAACNERTCTA
                     TVSATPDQIKTSGAVTLGQ"
     gene            2820..3788
                     /locus_tag="VMZ53_14960"
     CDS             2820..3788
                     /locus_tag="VMZ53_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29802.1"
                     /translation="MRHIGLTLVLVACAHNVPQDKATGPDGKQKGAVPVALVENEAKV
                     KGVVTYPGGDRIDWKKIELPQGTKGKLDLTLTWDTPRPGLRVAFDVFDQWNTPVVPAA
                     KKPAGRMQDASIDVAKGTYYVRIYAPRRKDAGSYKLVANFTPEAPAVPPDMTVPDPPK
                     LPAIPVAEPGCDVFDPSVKACEKVCPEFGAPAGWKACADKEKAEKEQQACEEAQKARI
                     EAMKNAPKSMDKRIIDVKVNGDEATITIAAGTDGQALLDKSWKGEVLNTQTGKPLVGG
                     TVTIVRVGKLQTLAKVNLSVDRLNQNPTVRLTPPAFDQPAVAACKR"
     gene            3812..4879
                     /locus_tag="VMZ53_14965"
     CDS             3812..4879
                     /locus_tag="VMZ53_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29803.1"
                     /translation="MLGKSGGLALGLLILFVAACPHNVPQDSKSGEDAKTKGAKPMTF
                     DNGEAKSSGIVTYPGGDRVDWKMIEIPEKQRGTLDIKLQWKPPRPGLQLQFDVFDEWN
                     TPIVQAKKQRGSKKSRSKIRTASIDGARGKYFIRVFAPDRGDAGAYKLTVEFKENNGP
                     LAIDLTKVEIPEPPKLAAIPEPEATCSEFDFDPKNKACMTVCPAAGAPAGWPGCKGKC
                     PDPPDANNPACWATMPCPNPPDRRVKACPKSKWPKCADINNPDPQNPNCDDAKADPVT
                     GRVIKTEIQGSDVLITISAGSDQGVGKGWRGKVLRGDGDSPLDGGDVSVIRVGKRDTL
                     GKVHLTTDQIGANPRVKLSPP"
     gene            4959..>5695
                     /locus_tag="VMZ53_14970"
     CDS             4959..>5695
                     /locus_tag="VMZ53_14970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29804.1"
                     /translation="MTQDPFVGKVIDGRYEIQQRVGEGGMGVVYKARQMSIDRVIALK
                     MLNQQMAGDQQWVQRFYNEAKACSRLQHPNTIRMFDFGQTQDGRLFMTMEFLDGMSLR
                     DALDRGPIAPQRVIKVLIQCCASLAEAHSIGIIHRDIKPDNVFLLNMAGSPDFVKLLD
                     FSVAKLLEGDRMKTQAGVVFGTPQYMSPEQGRGLPLDARSDLYALGILAFEMLTGNVP
                     YNDDNPMTVIQMHLHGQVPPMPDAVPYS"
BASE COUNT         1090 a         1845 c         1866 g          894 t
ORIGIN      
        1 gcgctcactc atcgtcgtcg ctctcgccta cgaccgggtc gatcccgtcg tttcgctcga
       61 tcgactgcgc ggccgcatcg cgcgatatgc gcgcggcgag gactaccacc tcgtcatgcg
      121 cgacaagctc gtgctgctcg ccgacagact ctcggcggcg ctcggccggc ccgtcgcttc
      181 gcgaccgtgc gtcgattcgg cgcccgtgct cgagcgcgag tgggcggagc gcggcgggct
      241 tggcttcgtc gcgaagaaca cgatggtgat cgcgccgggc ctcggcagct acatcgtcct
      301 cggcgagctg ctcgtcgatg tcgagctcgc aacgaccgca ccggcggagc ccgcgaagcc
      361 tcgatgcggc agctgtcgtg cgtgcctcga cgcgtgtccg acgggtgcgt tcgtcgacgc
      421 gtacgtgctc gatgcgcgga agtgcatctc gtatctgacg atcgagaacg agggcccgat
      481 tcccgtcgag ctgaggccgg cgatcggcac gtgggtcttt ggctgcgata tctgccaaga
      541 ggtctgccca ttcaacgccg gcgccggcga tgcacccgat ccgatgctgc ggccgcggac
      601 cggcgagcat gcgctgcccg atctcgttgc gctcgcgacg aagaacacca accaacttcg
      661 gcaattcgtg aagcgcacgg cgctgcgccg cgtgtcgcgt gagaatctgc tgcgcaacgt
      721 tgccgtcgcg ctcggcaaca ccggctcggc tgcggcgatt cctgcgctcg agcatctgct
      781 ccagaacaag agcgagctcg ttcgcgagca cgcgcaatgg gcgctcgatc acttgcgcgc
      841 gatggcgggg tgagcgccaa cgcgacggtg cgtacgcggt tcgcgccgcc cggcgagtgg
      901 gcacgacgta ttctcgcgaa tttgcgacat cgatcgtggc acctcgggtg cagcgcctcg
      961 ctgcatggcg accttgaccc gcaagcaatt cttgcagtcg gcgctcggcg cgtgcgccgg
     1021 cctcttcgga ctctcgatgc tctcgagctg caacgaccag acgatgccgc gcggcgacgg
     1081 tggcgtcgga gctgacagcg tcgggggcgc gggaggcgga ggcggaggag gaggaggagg
     1141 aggaggcggc ggtgagacgg tcgacgctgg cggaggcggc ggcggtggag gcggcaacga
     1201 gacggtcgac ggcggcacga cgaatcgctg cctcgccaac ggcacggtct cgacgatcgg
     1261 aaccaatcac ggtcacgtgc tcgtcgtgcc gaaagaggac gtcgctgctg gtaccgcgaa
     1321 gacgtacaac atccagggca cgtccggtca tccgcatacg gtgacgatca ggagcgaaca
     1381 cttcgcgcag ctcgcggagg aggaggcgat catgaccgtg tcgtcgtcgg acgccggcca
     1441 ttcacacgcc gtgatggtcg cctgcgcgta gtcgctactg atcgaggaac tcgggcgatg
     1501 ccacgtatgc cgccacggcc ttgatctgct cgtcggtcag cgcgccctcg aacgcgggca
     1561 tcagcttgtt ctgcgatccc ttgcgcactt ggttctcgac gagcgctggc gtgatccggt
     1621 gccgcagctc ctgcgaggtg agatctttca cgcccgtcgt cgcgaccgct tgcgcgctcg
     1681 gcttgccctt cggcccgtga caagccgcgc agatcgactg aaataccttc gcgccgtcgg
     1741 cgctgccgcc cgcgagcttt tgttcgcagc cagagagcgc gaccgtcgct acaaggccga
     1801 gcgcgtaccg cagcttcacg gtcacgacat gccacgtgct atgtttgctc gccatgggac
     1861 gggcttctgt tcttctgctg ctaattgtcg ctgcgtgcgg tggtgcgcag attcctcagc
     1921 acaacgggta caaggcgaaa gccaagccgt ggaagaaagc caagacgctc aagttcgatg
     1981 acaagagcga agccaaggca accggcgagc tgtcgtacgc cgagttcaag cgcgctgcgt
     2041 ggttcgtcgc cgatctgcag cagcccggcc agctcgacat caagctcgac atcacgccgc
     2101 ccggcgaaga agtcgacgaa gacttcgacc tcggcttcga ggtcctcgac gccggcaatc
     2161 gcgtgatcgc gcgctcgaac aaagaagagg gcgacgacac cggcgagatg cagaagagca
     2221 agtcgctcct cgatctcgag cccggtcgct acatgatcca tctctatctg caggatcgcc
     2281 gcgacacgtg tgactacgtc atgcgcgcga cgttcaagcc gatgagctcg gtcggtaaga
     2341 gcgacttccc ctcgcaggtg gcgttcacgc cgagcctgcc gatggtgccg ctccaggacg
     2401 acactccgaa aggctacaag tcgccgacga ccaccacgac cgtcgtcgtg aagaagccga
     2461 ggaccggcgg tggacagaag ccgcctccgc cgcctcctcc gcctcccccc accacgaagt
     2521 cggcgcgcat catcggcatg caggtcgtcg gtggcggcac gcagatcacg atcggtctcg
     2581 gcactgcggg cggcgcgtcc gtcggcatga agggcaagat caacaacgtc gcgagcggtg
     2641 gcttcacgct ggccgcctgc aacgaacgca cgtgcacggc gaccgtctcg gcgactcccg
     2701 accaaatcaa gacgtcgggc gccgttacgc tcgggcagtg atcggtcgtc atcgcgccaa
     2761 tctaggttgg atttgcgatc tcgcttgaac gagcgggatc cagcagctac caccaaggga
     2821 tgaggcacat cgggctaacg ctcgtgctgg tggcgtgcgc gcataacgtt ccgcaggaca
     2881 aggcgaccgg acctgacggc aagcagaaag gtgctgtgcc ggtcgctctc gtcgagaacg
     2941 aggcgaaggt caaaggtgtg gtcacctatc ccggtggcga tcgcatcgac tggaagaaga
     3001 tcgagctgcc gcaaggcacg aagggcaagc tcgatctgac gctgacgtgg gacacgccgc
     3061 gtcctggtct gcgcgtcgcg ttcgacgtgt tcgatcagtg gaacacgccg gtcgttcccg
     3121 ccgcgaagaa gcccgccggt cgcatgcagg atgcgtcgat cgacgtcgcg aagggcacgt
     3181 actacgtccg catctacgcg cctcgtcgca aagacgccgg cagctacaag ctcgtcgcca
     3241 acttcacgcc cgaagcgccg gcggtgccgc ccgatatgac ggtgcccgat ccgccgaagc
     3301 tgcctgcgat ccccgtggcc gagcccggct gcgacgtgtt cgatccctcg gtgaaggcct
     3361 gcgagaaggt ctgcccggaa ttcggcgcgc cggctggctg gaaggcgtgc gccgacaaag
     3421 agaaggccga gaaagagcaa caggcatgcg aggaagccca gaaggctcgc atcgaggcga
     3481 tgaagaacgc gccgaaatcg atggataagc ggatcatcga cgtgaaggtc aacggcgacg
     3541 aagcgacgat caccatcgcc gccggaaccg atggccaagc gctcttggac aagagctgga
     3601 agggcgaggt cctcaacacg caaacgggca agccgctggt cggcggcacg gtcacgatcg
     3661 tgcgcgtcgg caagctgcaa accctcgcca aggtcaacct cagcgtcgac cggctgaacc
     3721 agaaccccac ggttcgcctg actccgccgg cgtttgatca gcctgccgta gccgcttgta
     3781 agaggtagat tcgctggcat aacatcgagt tatgctcggg aaatcggggg gcctggccct
     3841 tggcctcctc atccttttcg tcgccgcgtg cccccacaac gttccgcagg acagcaagtc
     3901 cggcgaggac gcgaagacca agggcgcgaa gccgatgacg ttcgacaacg gcgaggccaa
     3961 atcctccggc atcgtgacgt atccaggtgg cgaccgcgtc gactggaaga tgatcgagat
     4021 tccggagaag cagcgcggca cgctcgatat caagctgcaa tggaagccgc cgcggccggg
     4081 tctgcagctc cagttcgacg tgttcgacga gtggaacacg ccgatcgtgc aggcgaagaa
     4141 gcagcgcggc agcaagaaga gccgctcgaa gattcgcacc gccagcatcg acggcgcgcg
     4201 cggcaagtac ttcatccgcg tgttcgcacc cgaccgcggt gacgccggcg catacaagct
     4261 caccgtcgag ttcaaagaga acaacgggcc gctcgcgatc gacctgacga aggtcgagat
     4321 tcccgagccg ccgaagctcg ccgcgattcc cgagccggaa gcgacgtgca gcgagttcga
     4381 tttcgatccg aagaacaagg cctgcatgac cgtgtgtccc gcagcgggtg cacctgcagg
     4441 ttggccgggc tgcaaaggca agtgccccga tccgccagac gcgaacaacc ccgcgtgctg
     4501 ggcgacgatg ccctgcccca acccgccgga tcgtcgcgtg aaggcgtgcc cgaagtcgaa
     4561 gtggccgaag tgcgccgaca tcaacaatcc tgatccgcag aacccgaact gcgatgacgc
     4621 caaggccgac ccggtcacgg gccgcgtgat caagaccgag atccaaggca gcgacgtgct
     4681 gatcacgatc tcggctggca gcgatcaagg cgtcggcaaa ggctggcgcg gcaaggtgct
     4741 gcgcggcgac ggcgactctc ccctcgacgg tggcgacgtc agtgtcattc gcgtcggtaa
     4801 gcgcgacacg ctcggcaaag tccatctcac caccgaccag attggcgcga acccgcgcgt
     4861 gaagttgagc ccgccgtgag cgaagcgaac cttccggata ggcgagcgct tcgcgagccg
     4921 aacgagacgg cgcgcccggg ttccgagacg ggggcgccgt gacgcaagac cccttcgtcg
     4981 gcaaagtcat cgacggtcgc tacgagatcc agcagcgggt cggcgaaggt ggaatgggcg
     5041 tggtctacaa ggcgcggcag atgtcgatcg atcgcgtcat cgcgctgaag atgctcaacc
     5101 agcagatggc gggcgatcag caatgggtgc agcgcttcta caacgaagca aaggcgtgct
     5161 ctcgcctcca gcatccgaac acgatccgca tgttcgactt cggccagacg caagacggcc
     5221 ggctgttcat gacgatggag ttcctcgacg gcatgtcgct acgcgacgcg ctcgaccgag
     5281 gcccgatcgc gccacagcgc gtgatcaaag tgctgatcca gtgctgcgca tcgctcgcgg
     5341 aagcgcactc gatcggcatc attcatcgcg acatcaagcc cgacaacgtg ttcctgctga
     5401 acatggccgg ctcgccggat ttcgtgaagc tcctcgactt ctcggtcgcg aagctgctcg
     5461 aaggcgatcg catgaagacg caagctggcg tcgtattcgg aacgccgcag tacatgtcgc
     5521 cggaacaagg ccgcggcctt ccactcgacg cgcgcagcga tctctacgcg ctcggcatcc
     5581 tcgcgttcga gatgctgacg ggcaacgtcc cgtacaacga cgacaacccg atgacggtca
     5641 tccagatgca cttgcatggg caggtgccgc cgatgcccga cgcagtgccg tactc
//
LOCUS       DATLBZ010000287         9818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1807227, whole genome shotgun sequence.
ACCESSION   DATLBZ010000287 DATLBZ010000000
VERSION     DATLBZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9818
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807227"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VMZ53_14975"
     CDS             complement(<1..854)
                     /locus_tag="VMZ53_14975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS29805.1"
                     /translation="MTFDVRAATEAERGDVAQLIREMIPGVAADARLAWLYEKNPAGR
                     ALTWIARENGELAGCTSYFPWRLVLDGEDRLCALGGDGYVRPKFRRRGLGALLHDAAR
                     DDMDRTGIGCMYGAPGAMNLTPLKHGGSREVGHVARWARPLRGAGIGVKPAPLDKLVR
                     EVLRPRRAAALEPMVREDERVDEVWRAFAPTLRLAAVRDATFFTWRFLDAPSGREPAF
                     VITDKGRPIGACALEVMNEGRSVRIVDVMAIPGEWHRCLSAIASHATTATDAHFVDIK
                     LMALDGRRR"
     gene            complement(920..3688)
                     /locus_tag="VMZ53_14980"
     CDS             complement(920..3688)
                     /locus_tag="VMZ53_14980"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS29806.1"
                     /translation="MSMIRVLATTVACVFALSCSAPSSPKFALKAAERRGRLESNGLR
                     FVIMPDDTTQLAQVDIRYDVGAREDPPGRAGLAHLVEHIMFQTRPDGPNTPPIFQTIL
                     DIATDMNAYTIWDMTHYHMASRTENLDAMLKIEAMRMYYGCQNIPEGEFEREREVVRN
                     EIRAQSSAEGQVVQYVEAAFYPKGHAYERMVGGNDEQIASASLQEVCDFMKKYYTPDR
                     AVLLVAGGVDVDGTIEMIKKWFAKIPKRDASPRVDVKPFTVTHDKKVIEADVERPMVF
                     IGWPLPAANTPEGELARFGIGRLVGEIAQKSSEYEFAYGVGADFLGGELAPLFIIEIE
                     LKDMSRLDEALEFAQKSAKQAYRGFDMAGYEANESFRKRSQAQFVQSLERLSNRTETV
                     ATMVQFSKDFDFDSTDMYLFHQLDKIAKFDIAKTSAVVKKYLAWENSGIVVVKPGTKG
                     IGQDKRSNVKYSAKSDAAFTDPPVDPAEAKRPIRNAGQLKNLDAAQRFKMKNGMDVVL
                     LPMHAMPLATASLVFKNAGDASTPSNPLLAAMAAEFLDFPMDAEVFGATGIGVNCGTT
                     DDYMQCNTRGINIYLDVMMKGLERKIKAGTYSQDGIEEWQKGVKAQFKLHSVQEQNEY
                     IRQVVTALYGPEHAYTKTAVLTPEYANKIHKDALDDFRDQHYRAGNATLIVVGDFDAK
                     YAEKLAKDTFGDWSTGTIDKPVDKNAAKRTGPAYVGVTKNKVDQQITVTLAYPAPGGI
                     DGQEAAREVLTRMLGIRAEDIRFKLGSTYGLTFARQTKVGPTSYMLRGGAVLGGTIDA
                     ERAGESIKVLRESLDALRNGDSFDEHFVRARRKLLQELLTESTVTGELAARLGKIAIY
                     NLDPNHYNTLIQQIAAVSPAQVKALIKQELNPQNEVLIMLGDRDHLNKTFKDVGITDV
                     KIIDPDYK"
     gene            3863..5530
                     /locus_tag="VMZ53_14985"
     CDS             3863..5530
                     /locus_tag="VMZ53_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HUS29807.1"
                     /translation="MSKDRDTAFFGHPAGLSTLFFTEMWERLSYYGARAFLFVYMTTA
                     AKLGGLEMSAGSAGLVMALYMSSVYLLSLPGGWIADRFLGQRKAVTFGGLGIMIGNIL
                     LAVPGDTMFYPGLAAIAIGTGLLKPNISTIVGQLYRKDDIRRDAGYTIYYMGINIGAG
                     IAPFVATLIAQSKSFRAFLESHHWDPNLCWKVGFAVPALGMAFGLVQYLIGYKKMGEA
                     GLHPHVPDDAKQAARDRTILIGIVGGIAGVIALGFGLNASGAVHLTGEMIGNIFGIGL
                     LVGAVLLFVGLYQTARNADERKGITAMIPLFIGAIAFFAIFEQASTTLSDYAERFVRR
                     EIVPGVELSSGGYQFFNAAFIVILATPFAFMWTRLAKSGKEPSSVLKFAIGMILAGVS
                     FAVLLPTLSSITELDHLQKAAPKDQIESVAPHLRVSGLYLVALYFVSTLSELFISPVG
                     LSSMNKLAPARLAGMVMGTWFLATAIGNYLAGRAAGFSESRGYSFLVYTLIISSFVVA
                     GVLFAVAPMIKRMMATRGNDKAQAPADRSEKAEPDPLPAARVTKESA"
     gene            5540..5734
                     /locus_tag="VMZ53_14990"
     CDS             5540..5734
                     /locus_tag="VMZ53_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29808.1"
                     /translation="MLPDARDGLTRLERIILWQLHEAQKELGRDSVPTALLYGRVVEH
                     INVSVDEFQAVLQRLVGSRY"
     gene            5735..6064
                     /locus_tag="VMZ53_14995"
     CDS             5735..6064
                     /locus_tag="VMZ53_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29809.1"
                     /translation="MTKKKIGELTVAITPSGRGFKGALYYEGGPLPMTDEERVLKQIK
                     GKTEDEVMTKANDWVAKRTPKKILICCERCKNGVELTKKTKKGAMEIYCGACGAVVYR
                     GAPIAWE"
     gene            complement(6065..7054)
                     /locus_tag="VMZ53_15000"
     CDS             complement(6065..7054)
                     /locus_tag="VMZ53_15000"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29810.1"
                     /translation="MADDMSLDDNIIALPPEGALTLGDVLSYQFERLRAIRDAPSDDD
                     IRAVLAREHYFKTWDRAMAHANERFDRRFEAAADAVVAGDLAALQGLLAEDPALATAR
                     SSYGHRQPLLHHVAQNGIESPRQKSGAPANMPDIARALLAAGADPNTTSQSYGGNDTV
                     LTLLCSSVHPHDAGVQAALVEVLVAGGADVNERDSAPLWTAITWGYAKAADALVRCGA
                     LPDNLVLAAGAGDLERTRAFADELDDTPRRDLRIPNTTKVLPARHLLEYALIYAASCN
                     RADVVELLLERGPDLTIREPIYGASAVSIAEHHLAARPEYGPLVELLRARSGG"
     gene            complement(7039..8574)
                     /locus_tag="VMZ53_15005"
     CDS             complement(7039..8574)
                     /locus_tag="VMZ53_15005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUS29811.1"
                     /translation="MTGEAKREALPTWVLALVTTAALLALVAYSAWQRWDALAVSPFP
                     LGVDGYFYPIQVRSLMEQHTLQYPASPLTFWFMLPFAQATDPIVGAKLGAAIGGALIA
                     LPAYAVGARLGRSRGAGYIAAVLASQLAASQYLSIEFVKQGFGLTVAMTALWLLLRAL
                     ERPSNARVLIALLGFVAALATHKLAGGLVVVVAVPGIIEEARGRGALRGRRLLYLMVG
                     LIATGGALVLLGLLLPQRFLGEQDVSLLRALFTDTPEWDLPALALPRYRLLFDREALI
                     GGVAAIAALVLVLVVPYDRKAYGPRIVAWISIVLALAIELPWLAVDDPQGLGFRLRLA
                     AFVPASLCAAIAIGAIGSLIKEPVAALLRDAILLVAAAILVVLIPVDRAEGRVLTHPA
                     LATAAMAASGEIPKNATVIVPERHILFMVAWYTRAKVSLHPDDVPPQERVRLMPLSFI
                     GMGSPLDRVLDEARREPGIAPPIGFHPRHRNGLVLVTEATWQWILARLGDQGTYWAKW
                     PTI"
     gene            8604..9278
                     /gene="queC"
                     /locus_tag="VMZ53_15010"
     CDS             8604..9278
                     /gene="queC"
                     /locus_tag="VMZ53_15010"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HUS29812.1"
                     /translation="MAKAVILLSGGLDSTTALAVAAQSFDCYALTVRYGQLHAVELDA
                     AKRVAALAGVKDHRVIDIDLAQLAASALTTPNVAVPKDRSLAEIGAPGDVPSTYVPAR
                     NTVLLSLALAWAESLGARDIFVGVNVLDASGYPDCRPEFIRAFEALAQVATRTGGFTV
                     HAPLIELTKAQIIELGVKLGVDYGLTHSCYDPSASGAACGRCDACQLRKKGFAEAGIA
                     DPTRYS"
     gene            9293..>9818
                     /locus_tag="VMZ53_15015"
     CDS             9293..>9818
                     /locus_tag="VMZ53_15015"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29813.1"
                     /translation="MKLGTLLLRNAAIGLSQLEAALRNQVLYGGRLGTNLVELGFIDL
                     ELLSTYLAELTGYPIATPTLLDDADKSLLDKLGSDDAHRLRSVPLGYLGEGTETVAVA
                     FVDPTDQLAMQTVEARLGAKVTPYVVPELRALYYLERHFGTPRRARFIRSTPRVGTDP
                     GELIGDDRRRSQPAG"
BASE COUNT         1618 a         3209 c         3252 g         1739 t
ORIGIN      
        1 cgacggcggc cgtcgagcgc catcagcttg atgtcgacga agtgcgcgtc ggtcgcggtc
       61 gtcgcgtgcg atgcgatcgc cgacaggcag cggtgccact cgccgggaat cgccatcacg
      121 tcgacgatgc gcacgctgcg gccttcgttc atgacctcga gcgcgcatgc tccgatcggg
      181 cggcccttgt cggtgatcac gaacgcgggc tctcggcccg acggtgcgtc gagaaaccgc
      241 cacgtgaaga acgtcgcgtc gcgaacggca gcgaggcgga gcgtcggcgc gaatgcacgc
      301 cacacttcat cgacgcgctc gtcttcgcgg accatgggct cgagcgcggc ggcgcggcgc
      361 gggcgaagca cttcgcgcac gagcttgtcg agtggtgcgg gctttacgcc gatgccggca
      421 ccgcggagtg ggcgcgccca gcgagcgaca tggccgactt cgcgcgagcc gccgtgcttg
      481 agcggcgtga ggttcatcgc gcccggtgct ccgtacatgc agccgatacc cgtgcgatcc
      541 atgtcgtcgc gagcggcgtc gtgcagcagg gcgccgagac ctcgacggcg gaacttcggg
      601 cggacgtagc cgtcaccgcc gagcgcgcaa aggcggtctt ctccgtcgag gacgagccgc
      661 cacgggaagt acgacgtgca gccggcgagc tcaccgttct cgcgagcgat ccacgtcagc
      721 gcgcggccgg cgggattctt ctcgtagagc cacgcgaggc gggcgtcggc agccactccg
      781 ggaatcatct cccggatgag ctgcgcgacg tcgccgcgct cggcttcggt tgctgcgcga
      841 acgtcgaacg tcacgccgca gagactagcc gggagcgaag gcggcgtgtt gtcacgcagc
      901 cccgctctct acggcctcac tacttatagt cggggtcgat gatcttgacg tcggtgatcc
      961 cgacgtcctt gaacgtcttg ttcaagtgat cgcggtcgcc gagcatgatc aggacttcgt
     1021 tctgcggatt cagctcctgc ttgatcagcg cctttacctg cgcaggcgag acggcggcga
     1081 tctgctggat cagcgtgttg tagtggttcg gatcgaggtt gtagatcgcg atcttgccga
     1141 ggcgggcagc gagctcgccg gtcaccgtcg attccgtgag cagctcttgg agcagcttgc
     1201 ggcgcgcgcg cacgaagtgc tcgtcgaagc tgtctccgtt gcggagagcg tcgaggctct
     1261 cgcggagcac cttgatcgac tcgccggcgc gttccgcgtc gatcgtgccg ccgaggacgg
     1321 cgccaccgcg cagcatgtac gacgtcgggc cgactttcgt ttgacgtgcg aacgtcagac
     1381 cgtaggtcga gccgagcttg aagcggatgt cttcggcgcg gatgccgagc atgcgagtca
     1441 gaacctcgcg cgcagcctct tggccgtcga tgccgcccgg cgcgggatac gcgagcgtga
     1501 ccgtgatctg ctgatcgacc ttgttcttgg tcacgccgac gtacgccggg cccgtgcgct
     1561 tggccgcgtt cttgtcgacc ggcttgtcga tcgtgccggt gctccagtcg ccgaacgtat
     1621 ccttcgcgag cttctcggcg tacttcgcgt cgaagtcacc gacgacgatc agcgtcgcgt
     1681 tacccgcgcg atagtgctgg tcacggaagt catcgagcgc gtccttgtgg atcttgttcg
     1741 cgtactcggg cgtcagaacc gccgtcttcg tatacgcgtg ctcgggtccg tagagcgcgg
     1801 tcacgacctg gcggatgtac tcgttctgct cctggacgga gtgcagcttg aactgcgcct
     1861 tgacgccctt ctgccactcc tcgatgccgt cctgggagta ggtgccggct ttgatcttgc
     1921 gctctaggcc cttcatcatg acgtcgaggt agatgttgat gccgcgcgtg ttgcactgca
     1981 tgtagtcgtc cgtcgtgccg cagttcacgc cgatgcccgt cgcgccgaac acttcggcgt
     2041 ccatcgggaa gtcgaggaac tcggccgcca tcgcggcgag tagcggattg ctcggcgtcg
     2101 acgcgtcacc ggcgttcttg aagaccaacg atgcggtggc gagcggcatc gcgtgcatcg
     2161 gcaagaggac gacgtccatg ccgttcttca tcttgaagcg ctgcgcggca tcgaggttct
     2221 tcagctgacc ggcgttgcgg atcgggcgct tcgcctcggc cggatcgaca ggcggatcgg
     2281 tgaacgccgc gtcgctcttc gccgagtact tcacattcga gcgcttgtcc tggccgatgc
     2341 ctttcgtgcc cggcttcacg acgacgatgc cggagttctc ccacgcgaga tacttcttca
     2401 cgaccgcgct cgtcttcgcg atgtcgaact tcgcgatctt gtcgagctgg tggaacaggt
     2461 acatgtcggt cgaatcgaag tcgaagtcct tcgagaactg gaccatcgtg gcgacggtct
     2521 cggtgcggtt cgacagacgc tcgaggctct ggacgaactg cgcctggctg cgcttgcgga
     2581 acgattcgtt cgcctcgtag cccgccatgt cgaagccgcg atacgcctgc ttcgccgact
     2641 tctgcgcgaa ctcgagcgcc tcgtcgaggc ggctcatgtc cttcagctcg atctcgatga
     2701 tgaacagcgg cgccagctcg ccgccgagga agtcggcgcc gacgccgtac gcgaactcgt
     2761 actcgctgct cttctgtgcg atctcgccaa cgaggcggcc gatgccgaac cgggcgagct
     2821 caccttcggg ggtgttcgca gctggaagcg gccagccgat gaacaccatc ggacgctcga
     2881 cgtccgcctc gatcaccttc ttgtcgtgcg tcaccgtgaa cggcttgacg tccacgcgcg
     2941 gcgacgcgtc acgcttcgga atcttcgcga accacttctt gatcatctcg atcgtgccgt
     3001 cgacatcgac gccaccagcg acgagcagca cggcgcgatc cggcgtgtag tacttcttca
     3061 tgaagtcgca gacctcttgc agcgaggcgc ttgcgatctg ctcgtcgttg ccgccgacca
     3121 tgcgctcgta cgcgtggcct ttcggataga acgcggcctc gacgtactga acgacttggc
     3181 cttccgcgct cgactgcgcg cggatctcgt tgcgaacgac ctcgcgctcg cgctcgaact
     3241 ccccttctgg gatgttctgg caaccgtagt acatgcgcat tgcctcgatc ttgagcatcg
     3301 cgtcgaggtt ctcggtacgc gacgccatgt ggtaatgcgt catgtcccag atcgtgtagg
     3361 cgttcatgtc cgtcgcgata tccaggatcg tctggaagat cggcggcgtg ttcggaccat
     3421 cgggacgcgt ctggaacatg atgtgctcga cgaggtgcgc gagacccgcg cgacccggcg
     3481 gatcttcacg cgcgccgacg tcgtaacgaa tgtcgacctg cgcgagctgg gtcgtgtcat
     3541 ccggcatgat cacgaagcga aggccgttgg actccaagcg tccgcgtcgc tcggctgcct
     3601 tcagcgcgaa ctttggcgac gaaggcgcag agcacgagag cgcgaacaca caggcgaccg
     3661 ttgtcgccaa cacccgaatc atcgacatgc gcgcgatctt tgccaaatcc gccgcgattg
     3721 caagctgtct tcgtcggtgc acccgcctga cggtaaatga cctagggagc gaccgggcac
     3781 ctcgctggga caccccccag tcgcatacca actcgtgcat ttcgcagtca cgaggtgcgc
     3841 gaattgcgat aggttgcgcg cgatgtccaa ggacagggat acggcgttct tcggccaccc
     3901 ggctggcctc tccacgctgt ttttcaccga gatgtgggag cgcctttcgt attacggcgc
     3961 gcgcgcgttc ctcttcgtct acatgacgac ggccgccaag ctcggcggtc tcgaaatgtc
     4021 cgcggggtcg gcgggcctcg tgatggcgct ctacatgtcg agcgtctatc tgctctcgct
     4081 gcccggtggc tggatcgcgg atcgcttcct cggtcagcgc aaggccgtca cgttcggcgg
     4141 cctcggcatc atgatcggca acatcctgct cgcggttccc ggcgacacga tgttctaccc
     4201 gggcctcgca gcgatcgcga tcggcaccgg cctcctgaag ccgaacatct cgacgatcgt
     4261 cggccagctg tatcgcaagg acgatatccg tcgcgacgct gggtacacga tctattacat
     4321 gggcatcaac atcggcgccg gcatcgcgcc gttcgttgcg acgctcatcg cacagagcaa
     4381 gtcgttccgc gcattcctcg agagccacca ctgggatccg aacctctgtt ggaaggtcgg
     4441 cttcgcggtt cccgcgctcg gcatggcctt tggtctcgtg cagtacctca tcggctacaa
     4501 gaagatgggt gaagccggcc tgcatccgca tgtgccggac gatgcgaagc aagcagcgcg
     4561 tgaccgcacg attctgatcg gcatcgtcgg cggcatcgct ggcgttatcg cgctcggctt
     4621 tggtctgaat gccagcggtg cagtgcacct cacgggcgag atgatcggca acatcttcgg
     4681 catcggtctc ctcgtcggtg cggtgctgct gttcgtcggc ctctatcaga ccgcgcgcaa
     4741 cgccgacgag cgcaagggca tcacggcgat gatcccgctc ttcatcggcg cgatcgcgtt
     4801 cttcgcaatc ttcgagcagg cgagcacgac gctctctgac tacgccgaac gcttcgtccg
     4861 ccgcgagatc gttccgggcg tggagctgtc gtcgggcggc tatcagttct tcaacgcggc
     4921 attcatcgtg atcctcgcga cgccgtttgc gttcatgtgg acgcgcctcg cgaagtcggg
     4981 caaggagccg tcgtcggtct tgaagttcgc gatcggtatg atcctcgcgg gcgtgtcatt
     5041 cgcggtgctg ctgccgacac tctcgtcgat caccgagctc gaccatctcc agaaggccgc
     5101 cccgaaggat caaatcgaat cggtcgcacc gcacctgcgc gtcagcggcc tctacctcgt
     5161 agctctctac ttcgtgtcca cgctgtccga gctgttcatc tcgcctgtag gcctgtcgtc
     5221 catgaacaag ctcgcgcctg cgcgtctcgc cggcatggtc atgggcacgt ggtttctcgc
     5281 cacggcgatc ggcaactacc tcgcgggtcg cgccgcaggc ttctccgagt cgcgcggcta
     5341 ctcgttcctc gtctacacgc tgatcatctc gtcgttcgtc gtcgccggcg tgctgttcgc
     5401 cgtcgcgccg atgatcaagc gcatgatggc cacgcgcggc aacgacaagg cgcaagcgcc
     5461 ggccgatcgc tccgagaagg ccgagccgga tccgcttccg gctgcgcgcg ttaccaaaga
     5521 gtccgcctag tagcccgcca tgctgcccga cgcgcgcgat ggcctcactc gcctcgagcg
     5581 catcatcctc tggcagctgc acgaagcgca gaaggagctc ggccgcgact cggtgccgac
     5641 ggcactgctc tacggccgcg tcgtcgagca catcaacgtc agcgtcgacg agtttcaagc
     5701 ggtgctgcag cgtttggtag gctcgcgcta ctagatgacg aagaagaaga tcggggagct
     5761 gacggtggcg atcacgcctt cgggacgcgg gttcaagggc gcgctgtatt acgagggtgg
     5821 tccgctcccg atgaccgacg aagagcgcgt gctcaaacag atcaagggca agaccgaaga
     5881 cgaggtcatg acgaaagcga atgactgggt cgccaagcgc acgccgaaga agatcctcat
     5941 ctgctgcgag cgctgcaaga acggcgtcga gctgacgaag aagacgaaga agggcgcgat
     6001 ggaaatctac tgcggcgcct gcggtgccgt cgtctatcgc ggcgcgccga tcgcgtggga
     6061 gtagctagcc gcccgagcgt gcgcgcagca gctcgacgag ggggccgtac tcggggcgag
     6121 cagcgaggtg atgctcggcg atgctgacgg cgctcgcgcc gtagatcggc tcgcggattg
     6181 tcaggtcggg cccgcgctcg agcagcagct cgacgacgtc tgcgcggttg cagcttgcgg
     6241 cgtagatcag ggcgtactcg aggaggtggc gcgcggggag caccttcgtg gtgttcggaa
     6301 ttcggaggtc gcggcgcggc gtgtcgtcga gctcgtcggc gaatgcacgc gtgcgctcga
     6361 gatcgccggc accggctgcg agcacgaggt tgtcgggcag cgcgccacag cggacgagcg
     6421 cgtcggccgc cttcgcgtag ccccacgtga tcgcggtcca cagcggcgcg ctgtcgcgct
     6481 cgttgacgtc agcaccgcct gcgacgagga cctcgacgag cgccgcctgc acgcccgcgt
     6541 cgtgcggatg cacggacgag cagagcagcg tgagaaccgt gtcgttgcca ccgtagctct
     6601 gtgatgtcgt gttcgggtca gcgccggcag cgagcaacgc acgtgcgatg tcgggcatgt
     6661 tcgcgggcgc gccggatttc tgccgcgggg actcgatgcc gttctgcgcg acgtgatgca
     6721 ggagcggctg gcgatggccg tagctcgagc gcgccgtcgc gagcgccggg tcttcggcga
     6781 ggaggccctg cagcgctgcc agatcgcccg cgacgaccgc atcggccgcg gcttcgaatc
     6841 gtcgatcaaa gcgctcgttg gcgtgtgcca tcgcgcggtc ccacgtcttg aagtagtgct
     6901 cgcgtgcgag gactgcgcgg atgtcgtcgt cggaaggagc gtcgcgtatc gctcgaagcc
     6961 gctcgaattg atacgacagc acgtcgccga gcgtcagcgc accttcgggt ggcagcgcga
     7021 tgatgttgtc gtcgagactc atatcgtcgg ccacttcgcc cagtacgtac cctgatcgcc
     7081 gagccgcgcg aggatccact gccacgtcgc ttcggtgacg agcacgaggc cgttgcgatg
     7141 gcgtggatga aagccaatgg gcggcgcgat gccgggctcg cggcgtgctt cgtcgaggac
     7201 gcggtcgagc ggtgatccca tgccgatgaa cgacagcggc atcaagcgca cgcgctcctg
     7261 tggcggaacg tcgtccggat gaagcgaaac ctttgcgcgc gtgtaccaag ccaccatgaa
     7321 gaggatgtgg cgttcgggaa cgatcaccgt cgcgttcttc gggatctcgc cgctcgccgc
     7381 catcgccgcc gtcgcgagcg cgggatgcgt gagcacgcga ccctccgcgc ggtcgaccgg
     7441 aatcagcacg acgaggatcg ccgcggcgac gagcaggatc gcatcgcgca gcagcgccgc
     7501 gacaggctcc ttgatgagcg agccgatcgc gccgatcgcg attgctgcac acagcgacgc
     7561 cggcacgaac gcggcaagtc gaagtcgaaa gccgaggccc tgcggatcgt cgacggcgag
     7621 ccatggcagt tcaatcgcga gcgcgagcac gatcgagatc cacgcgacga tgcgcgggcc
     7681 gtacgccttg cggtcgtagg gcacgacgag gacgagcacg agcgcggcga tcgcggcgac
     7741 cccgccgatc aatgcttcgc gatcgaacag caagcgatag cgaggcaacg cgagcgcagg
     7801 cagatcccac tcgggcgtgt cggtgaacag cgcgcgcaga agcgagacgt cctgttcgcc
     7861 gagaaaccgc tgcggcaaca gcaacccgag caacacgagc gcaccgccgg tggcgatcaa
     7921 gccgaccatc aagtagagga gccgtcgtcc acgcaacgcg ccgcgacctc gcgcttcctc
     7981 gataatgccg ggcaccgcga cgacgacgac gagcccgccg gcgagcttgt gcgtcgcgag
     8041 cgccgcgaca aacccgagca gtgcgatcag gacacgagcg ttcgacggcc tctcgagcgc
     8101 gcgcagcagc agccacagcg cagtcatcgc gacggtgagg ccgaagccct gcttgacgaa
     8161 ttcgatcgac aagtactgcg atgccgcgag ctgcgacgcg agcacggccg cgatgtagcc
     8221 cgcgccgcgc gagcgtccga ggcgcgcacc gaccgcatac gccggcaacg cgatgagcgc
     8281 gccaccgatc gccgcaccca gcttcgcgcc gacgatcgga tcggttgcct gcgcgaacgg
     8341 cagcatgaac cagaacgtca gcggcgatgc gggatactgc agggtgtgct gctccatcag
     8401 cgagcgcacc tgaatcggat agaagtaacc gtcgacgcca agtggaaacg gcgagacggc
     8461 gagcgcatcc cagcgctgcc atgctgaata cgcgacaagg gcgagcagcg ccgccgtcgt
     8521 cacgagcgcg agcacccacg tgggtagagc ctcgcgcttc gcctcgccgg tcacgcacga
     8581 aggatacgcg ctaggatgcg cgcgtggcga aggccgtcat tcttctctcc ggtgggctcg
     8641 actcgacaac cgcgctagcc gtcgcggcgc agtccttcga ttgctacgcg ctgacggtgc
     8701 gctacgggca gctgcacgcg gtcgagctcg acgcagcgaa gcgtgtcgca gcgctggccg
     8761 gcgtgaaaga tcaccgcgtg atcgacatcg atctcgcgca gctcgcggca tcggcgttga
     8821 ccaccccgaa cgtcgccgtg ccgaaggatc gttcgctcgc cgagatcggc gcacccggcg
     8881 acgtgccgtc gacgtacgtg cccgcgcgta acacggtgct gctttcgctc gcgctcgcgt
     8941 gggcggagtc gctgggcgcg cgcgacatct tcgtcggcgt gaacgttctc gatgcgagcg
     9001 gctatcccga ttgccggccc gagttcatcc gcgcgttcga agcgctcgca caggtcgcga
     9061 cgcgcaccgg cggcttcacc gtgcacgcgc cgctgatcga gctcaccaag gcgcagatca
     9121 tcgagctcgg cgtgaagcta ggtgtcgact acggactgac gcacagctgc tatgacccgt
     9181 cggcgagtgg cgctgcgtgt ggtcgctgcg acgcgtgcca gcttcgcaag aagggctttg
     9241 ccgaagcggg catcgccgat ccaactcgct attcgtagta ccctgacgag ggatgaagct
     9301 cggcacgctg ctcctgcgca acgcggcgat cggcttgtcg cagctcgaag cagcgctgcg
     9361 caatcaggtg ctctacggcg gccggctggg cacgaatctc gtcgagctgg gcttcatcga
     9421 tctcgagctg ctgtcgacgt acctcgcgga gctcaccggg tatccgatcg cgacgccgac
     9481 gctcctcgac gacgccgaca agtcattgct cgacaagctc ggctccgacg atgcgcatcg
     9541 gctgcggtcc gtgccgctcg gctatctcgg cgagggcacg gagacggtcg cggtggcctt
     9601 cgtcgatccg acggaccagc tggcgatgca gaccgtcgag gcccggctcg gcgcgaaggt
     9661 cacgccgtat gtggtgcccg agcttcgcgc gctctattac ctcgagcgtc acttcggcac
     9721 gccgcgccgc gcgcgcttca ttcgcagcac tccgcgcgtc ggcaccgatc cgggcgagct
     9781 catcggcgac gatcgcagac gcagccagcc cgccggcg
//
LOCUS       DATLBZ010000288        10430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8357975, whole genome shotgun sequence.
ACCESSION   DATLBZ010000288 DATLBZ010000000
VERSION     DATLBZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8357975"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VMZ53_15020"
     CDS             complement(<1..1297)
                     /locus_tag="VMZ53_15020"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HUS29814.1"
                     /translation="MKKLGLLLVVLAACKGSDPTPQSAKASSTKAAPEDPWAKKSGTD
                     NIKDPALKRLVELQQNGPGKDKYPQADAVVALEQDDVTYKPDGTIITKHHSIVKIIDP
                     QRGKEKFADLHIPYDSKRQTLEVELARTVNDDGKPEVASKDEIGDIVPPYLADATIYS
                     DVRERVVTFPAVDAGSVLELVYTRTSKPSADSPMGGEQMLGRWNPILERVVTITAPSN
                     VTPKFAVEGMKLDPKQSDASGNHTWTFRIENQPDRQPEMGSLSDAAVLPRLIYGFAPS
                     WQKVLEPVAGRYLDKAVPNPLPEAIKAEADRVVAGAKDEAEKAQKLYAFVAHDIRSID
                     LPLGAAGYEPHAPEVVLQNRYADDRDKVGLLLAMASSQGIRGRAVLVRTGKVPVISSV
                     PTLAQFDRMIAKLDVGSKDVWLDPSDENGQYAVAFAGQDN"
     gene            complement(1300..3219)
                     /locus_tag="VMZ53_15025"
     CDS             complement(1300..3219)
                     /locus_tag="VMZ53_15025"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HUS29815.1"
                     /translation="MLCAVLLCISVAPASAGVDPEILDAIKKVKPADYPSANTVSVIS
                     SQSVVFQPDGQFTNTMHQATLVLTTAGKEQAASASFYYTKDAEKMEILEAQVVKPDGK
                     VVTIDKSEFQDVEQSGEANIYDPQGRALKVTFKNLAVGDVVDITTKLTRLLPTRMNYF
                     NDVFGFQSTDPILHASYNVDGPASRPLTTKIYHQERTSPIKASKTKTGDRVLYSWSVT
                     NAPQLVPEVAMNFRTEVPMLVVSTDPSWKNFSKWWGELTEPLMKPTPEIQTKVAELIK
                     DSKTDDEKIRALYNFVAQDIRYRGLGVGPRTGYTPRPAHDTMTSRWGVCRDVSILLTS
                     MLREAKIEAYPVITNAGDPVLEDIAHDGFNHAIVAIKKPGGWEYVDPTAKNNNALLPG
                     NEAEQHTLVATLKGEPLTKIPAADPSVNMGHAIAETTINADGSLSSKVKLQTKGMFDL
                     VVRSVGAMMSKDQQKEMLETLLHHSLPDAELVSFEMTSALALFTPMEVSLEIKVPNAA
                     PKTGDYRLLRSIVTSGSLGLVENVLPQVLGAQPTRKYGLDAQVTFEYDQTETIKLPAD
                     TKIVALPNSAQSSNKVSAVTSSCTKQDATTISCKRTFQLKSRHIAPDQYKTLRTSLAS
                     MALIGHQPVILAGGK"
     gene            complement(3394..4536)
                     /locus_tag="VMZ53_15030"
     CDS             complement(3394..4536)
                     /locus_tag="VMZ53_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29816.1"
                     /translation="MATALARRYRAIAEADSHSELEVTIGEARQIYPEMWRHLDEARI
                     ALGERGVDCTAYDNLRRQELGTIGVDVGMTVGVEFYTYGLKVGEIYKKTASFDADGAH
                     RAIAAIAILRQLMPEVDWAARARAEDQLIREVGSLKSSTWIGLAKLAGAAAAIAIVAL
                     VIFHFLSDKGSEIENAPRPVTAPVVETPKPVGPPDPVPLAVRAQCQKARQTITERIAK
                     EHDLVRGPQWSMNCEGILIDNQPVAFAVSVHAPSRGGALIELRGVVSLNGMRDLRPFL
                     PVPAATALAFVGDLDGDTNDELVFVGPQSLVVTRITKDGFVDIAGPAMPTGCAADAKV
                     TGDYRNGRPNERNILVLTAPDDQVGSGCLSAGKHYFRLAGDHLEET"
     gene            4658..5620
                     /locus_tag="VMZ53_15035"
     CDS             4658..5620
                     /locus_tag="VMZ53_15035"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HUS29817.1"
                     /translation="MIKRPKIAIVGAGGNVGAAVAQWAVQKELGDLVLIDVKPGPAEG
                     RALDLTQCGPWEGFNSTSFVATDKPEMMAGADVVVMTAGVPRKPGQSREELIGINAGI
                     VKGICENVKKHAPDAVLIVVSNPLDAMVFVAKQVTGFPRERVIGSAGVLDSARFRTYL
                     AMAAGVSVEDVHAIVLGAHTDKDMVPITSNATIGNVPVTKFLAADKLNEVVENTKKGG
                     ATLTGLIGTSAWLAPGAGTCLMVEAIVRNQGRVLPCSIELNGEFGVKGAFLGVPCKLT
                     AKGAEAVYEFELSAQEKDAFGKALAANTELMNIARGLLSGNTPG"
     gene            5813..6766
                     /locus_tag="VMZ53_15040"
     CDS             5813..6766
                     /locus_tag="VMZ53_15040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29818.1"
                     /translation="MREAMHSLNTLVGSIVEDRYEIQEELGAGAMGVVYRGRHLKVGR
                     TVAIKVLHDHLVKDPSMVERFEDEARAVARLRHRNLVGVLDVGVTDDGQRCMVLEFAP
                     GCSMAKLVTVAMKRERVIALVRQLLLGLEHAHEAGLVHRDLKPENVLVERSADGTEIP
                     RIVDFGIAQLRDRADRGDGRRHTTDGLVVGTPMYMSPEQARGEAVDERTDLFALGVMV
                     YEMLAGCTPFEGSGVEVMMLNIMQDPPSIATRTGRDVDAQLEAFARKLMARDKADRYA
                     SATDALAALDAIASNAVPRPVVDNPTVRHRTTRITMPALKR"
     gene            6907..8550
                     /locus_tag="VMZ53_15045"
     CDS             6907..8550
                     /locus_tag="VMZ53_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29819.1"
                     /translation="MIGSIVEDRYLVEEELGTGAMGRVFAARHVKVGRRVAIKIMHDE
                     LAKNAAIVERFAREAMVAARLRHPNLVSVLDVGQTAMHQPLLVLELAPGIPLSHMTCE
                     PLHGPRVVNLIRQLLHGLSHAHTAGLIHRDLKPDNILVETTADGLEIPRIVDFGIAVA
                     ADRDDTVAGRRLTDANMVIGTPFYMSPEQARAKDIDHRTDLFSLGVIMYELLAGMPPF
                     PGSSLDVALANVSKDAPTIAERAGIDVDPLLEAFMRKLMARRLETRFSSAREALDVLA
                     LIEIDRMAAARVLGGSPLVEVPRPMLAQGSAPYAIPTPPNSDELTTITEPPVASDEST
                     PTEHVDPTDDDFSDLEFDPARSQRRRKRLALGVVFAALTAVVAVFALQAPPRVDAPAV
                     DVASQDDASMAIRPVQTKAPLLPTTSVAVVKKQHVARPTTKPVTAPNVVAPPPMMVAA
                     PMIVAAPRAPTMVLAAAPVIAPTAAPAAAPPVAIDSVDALVAKYQAVAKKLKHSAAGP
                     QTEDLWMRFRLIRINESIATQQTRRQALALLGAIDNAIE"
     gene            complement(8578..9480)
                     /locus_tag="VMZ53_15050"
     CDS             complement(8578..9480)
                     /locus_tag="VMZ53_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29820.1"
                     /translation="MRTAVHVVVTIGLLGACNGDGPKGDMGEMGTMGIEGSPGPQGPA
                     GPQGPAGPQGPAGPQGSAGPQGATGATGSQGAQGPQGPAGADGAMGPQGPQGIQGPVG
                     PQGPAGGSSPITFTLSAVSEQLPYTTPTVVSNPTACTNIPLGFTYTGFGASTSTISVS
                     PYGMLFLGTGCALSSSNTALPSSLSTDPFVAYLWDLNNNNNGGWFQYHTHGTSPGRAF
                     SLNHRCTTNFPESAVVETTITLFESTGLITVQYHLYSGSVAGGGATIGMQTAGGSNAT
                     ARMISLNVPMISPTSTALSFQPSP"
     gene            complement(9571..>10430)
                     /locus_tag="VMZ53_15055"
     CDS             complement(9571..>10430)
                     /locus_tag="VMZ53_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS29821.1"
                     /translation="QTGQRVTWLGHSTVLLELDGRRVLTDPVFANRIGPSPSLVGPRR
                     FHPTPAQLSELPKLDVILLSHDHFDHLCAPTMREIAKLDVPIVTALGVGRYLEKYGVA
                     ASRITELDWHESATVGGIRFTATPSQHFSGRSIGGRNKTLWAAWVIESERHKIFFSGD
                     TGLTKEFIETGRQYGPFDLVMLEIGAFHPSWGTIHLGPANALEAFKMLGGGTLLPVHW
                     GTFSLGFHAWNEPGEELMALAPKHDARILTPRLGQVFEPDHIEGPTPWWRDLVPARDR
                     EALIAAAAK"
BASE COUNT         1731 a         3354 c         3464 g         1881 t
ORIGIN      
        1 ggttgtcctg gccggcgaat gcgaccgcgt actggccgtt ctcgtcggac ggatcgagcc
       61 aaacgtcttt gctgccgaca tcgagcttcg cgatcatgcg atcgaattgc gcgagcgtcg
      121 gcacgctcga gatgacgggc accttgcccg tgcgcacgag caccgcgcgg ccacgaatgc
      181 cttgcgacga tgccatcgcg agcagcaggc cgactttgtc gcggtcatcg gcgtagcgat
      241 tctgcagcac gacttcgggt gcatgcggct cgtagcctgc agcaccgagc ggcagatcga
      301 tcgagcgaat gtcatgcgcg acgaacgcgt agagcttctg cgccttctcc gcttcgtcct
      361 tcgcgccggc gacgacgcga tcggcctcgg ccttgatcgc ctctggcagc ggattcggga
      421 cggccttgtc gaggtaacga ccggcgacgg gctcgagcac cttctgccag ctcggcgcga
      481 acccgtagat caggcgcggc agcacggccg cgtcggatag cgagcccatc tcgggttggc
      541 gatccggctg gttctcgatg cggaacgtcc acgtgtggtt gccgctggcg tcgctctgct
      601 tcgggtcgag cttcattcct tcgaccgcga acttcggcgt gacgttcgac ggagcggtga
      661 tcgtgacgac gcgctcgagg atcggattcc agcggcccag catctgctcg ccacccatcg
      721 gcgaatccgc gctcggcttg gacgttctcg tgtagacgag ctcgagaacg ctgccggcgt
      781 cgaccgcggg gaacgtgacc acgcgctcgc ggacgtcgga gtagatcgtc gcgtcggcga
      841 ggtacggcgg cacgatgtcg ccgatctcgt ctttgctcgc gacctcgggc ttgccgtcgt
      901 cgttgacggt gcgcgcgagc tcgacttcga gcgtctggcg cttgctgtca tacgggatgt
      961 gcagatcggc gaacttctct ttgccgcgct gcggatcgat gatcttcacg atcgagtgat
     1021 gcttcgtgat gatcgtgccg tcgggcttgt aggtcacgtc gtcttgctcg agcgcgacga
     1081 cggcatcggc ctgcggatac ttgtccttgc ctggaccgtt ctgctgcagc tcgacgaggc
     1141 gcttgagcgc cggatccttg atgttgtcgg taccgctctt cttcgcccac ggatcctccg
     1201 gggcagcctt cgtcgacgat gctttcgccg actgcggcgt tggatcgcta cctttgcagg
     1261 cggcgagcac gactagcagg agacctagct tcttcatgac tacttgcctc ccgcgaggat
     1321 caccggctgg tgaccgatga gcgccatcga cgcgagcgac gtgcgcagcg tcttgtattg
     1381 atcgggcgcg atgtgccggc tcttgagctg gaacgtgcgc ttgcagctga tcgtcgtcgc
     1441 atcctgcttc gtgcacgacg acgtcaccgc cgacaccttg ttcgacgact gcgcggaatt
     1501 cggcagcgcg acgatcttcg tatcggcggg caacttgatc gtctcggtct ggtcgtactc
     1561 gaacgtgacc tgcgcatcga ggccgtactt ccgcgtcggc tgggcgccga gcacttgcgg
     1621 cagcacgttc tcgacgaggc cgagcgagcc gctcgtcacg atcgatcgca gcaggcggta
     1681 gtcgcccgtc ttcggcgcag cgttcggcac cttgatctcg agcgacactt ccatcggcgt
     1741 gaacagcgcg agcgcgctcg tcatttcgaa cgacacgagc tcggcatccg gcagcgagtg
     1801 gtggagcagc gtctcgagca tctccttctg ctggtccttg ctcatcatcg cgccgaccga
     1861 acgcacgacg agatcgaaca tgcccttcgt ctgcagcttc accttgctcg acagcgagcc
     1921 atcggcgttg atcgtcgtct cggcgatcgc gtggcccatg ttgacgctcg ggtccgccgc
     1981 cggaatcttc gtgagcggct cgcccttcag cgtcgcgacg agcgtgtgct gctcggcttc
     2041 gttgccggga agcagcgcgt tgttgttctt cgccgtcggg tcgacgtact cccagccgcc
     2101 cggcttcttg atcgcgacga tcgcgtgatt gaagccgtcg tgcgcgatgt cctcgagcac
     2161 cgggtcaccg gcgttcgtga tcaccggata cgcttcgatc ttcgcttcgc gcagcatcga
     2221 cgtcagcagg atcgacacgt cgcggcagac gccccaacga gaggtcatcg tgtcgtgcgc
     2281 gggacgcggc gtgtagccgg tgcgcggacc aacgccgagg ccgcgatagc gaatgtcctg
     2341 cgcgacgaag ttgtagagcg cgcggatctt ctcgtcgtcg gtcttcgaat ccttgatgag
     2401 ctcggcgacc ttggtctgga tctcgggcgt cggcttcatg agcggctcgg tcagctcgcc
     2461 ccaccacttc gagaagttct tccagctcgg atccgtcgag acgacgagca tcgggacttc
     2521 ggtgcggaag ttcatcgcaa cttccggaac gagctgcggc gcgttcgtga cgctccagct
     2581 gtagagcacg cggtcgcccg tcttcgtctt gctcgccttg atcggtgacg tgcgctcctg
     2641 gtgatagatc ttcgtcgtga gcggccgcga tgccggaccg tcgacgttgt agctggcgtg
     2701 caggatcggg tcggtcgact ggaagccgaa cacgtcgttg aagtagttca tgcgcgtcgg
     2761 cagaaggcgc gtgagcttcg tcgtgatgtc gacgacgtcg ccgaccgcga ggttcttgaa
     2821 cgtgaccttc agcgcgcggc cctgcgggtc atagatgttg gcctcgccgg actgctcgac
     2881 gtcttgaaac tccgacttgt cgatcgtgac gaccttgccg tcgggcttga cgacctgcgc
     2941 ctcgaggatt tccatcttct cggcgtcctt cgtgtagtag aagctcgcgc tcgcggcttg
     3001 ctccttgccc gccgtggtca gcacgagcgt cgcttgatgc atcgtgttcg tgaactggcc
     3061 gtcgggctgg aacacgacac tctgcgacga gatcacactg acggtgttcg cggacggata
     3121 gtccgcgggc ttcaccttct tgatcgcgtc gagaatttca gggtccacac ccgcgctcgc
     3181 aggcgctaca gagatgcaca gcagaacggc acagagcacg tgtcgcatcg tcggtcctcc
     3241 aatggctgtc gaggttcgta ccacggcaac cttcgagggc ccggaagttc aggctcgcac
     3301 ctcctgaagt catgtgatgc gacgaggcga tgcggcgttc tctgggcgtc gcacccccgt
     3361 cacacccgac ctcagccgcg ccgatttggc tgtctaggtc tcttcgaggt ggtcgccggc
     3421 gagcctgaag tagtgcttgc cggcagagag gcatcccgag ccgacttggt cgtccggtgc
     3481 cgtcaagacg aggatgttgc gctcgttcgg ccggccattg cggtaatcgc ccgtgacctt
     3541 ggcgtcggcg gcgcagccag tcggcattgc aggccccgca atatcgacaa agccgtcctt
     3601 cgtgatgcgc gtgacgacga gcgattgcgg cccgacgaac accagctcat cgttcgtatc
     3661 accgtcgagg tcgcctacaa atgcgagcgc ggtggccgcg ggaacgggaa ggaacggccg
     3721 caggtcgcgc atgccgttga gcgacacgac gccgcgcagc tcgatgagcg caccaccgcg
     3781 cgacggcgcg tgcacagaca cggcgaacgc gaccggctgg ttgtcgatga ggatgccttc
     3841 gcagttcatc gaccattgcg ggccgcgcac gaggtcgtgc tccttcgcga tgcgttctgt
     3901 aatcgtctgc cgcgccttct gacattgcgc gcgtaccgcg agaggcaccg gatcgggtgg
     3961 gccgaccggc ttcggcgttt cgacgacggg cgctgtcacc ggtcgaggag cattctcgat
     4021 ctcggagccc ttgtccgaga ggaagtggaa gatcacgagc gcgacgatcg cgatcgccgc
     4081 cgcggcgcct gccagcttcg cgaggccgat ccacgtcgag gacttcagcg agcccacctc
     4141 gcgaatgagc tggtcttcgg cgcgtgcgcg tgccgcccag tcgacctcgg gcatcagctg
     4201 ccgcagaatc gcgatggctg cgatcgcgcg gtgggcgccg tcggcgtcga agctcgccgt
     4261 cttcttgtag atttcgccga ccttgagacc gtacgtatag aattcgacgc ccacggtcat
     4321 gccgacatcg acgccgatcg taccgagctc ctggcggcgc agattgtcgt aggcggtgca
     4381 gtcgacgcca cgctcgccga gcgcgatgcg ggcctcgtcg agatgtcgcc acatttctgg
     4441 atagatctgc cgggcctcgc cgatcgtcac ctcgagctcc gagtgagagt cggcctccgc
     4501 gatcgcgcga tatcggcgcg ccagggccgt cgccacgcca accgattgaa ccgggtccgc
     4561 catgccccga gtcatccaac gatccggggc cgcggagcaa tctgatttgc tgtgacattg
     4621 cagcgtcgac ggcgcggggg tatgacccgg accaatcatg atcaagcgtc cgaagatcgc
     4681 catcgtcggc gccggcggca acgtcggtgc agcagttgct caatgggccg ttcagaagga
     4741 gctcggtgac ctcgtcctca tcgacgtcaa gccgggcccc gcagagggtc gtgcgctcga
     4801 cctgacgcag tgcggcccgt gggagggctt caacagcacg agcttcgtcg ccacggacaa
     4861 gcccgaaatg atggccggcg ctgatgtcgt cgtgatgact gccggcgtgc cgcgcaagcc
     4921 gggtcagtcg cgcgaagagc tgatcgggat caacgcgggc atcgtcaaag gcatctgcga
     4981 gaacgtcaag aagcacgcgc ccgacgccgt gctgatcgtt gtctcgaacc cgctcgacgc
     5041 gatggtgttc gtcgcgaagc aggtgactgg cttcccgcgc gagcgcgtga tcggctcggc
     5101 cggcgtgctc gactcggcgc gcttccgtac gtacctcgcg atggccgcgg gcgtcagcgt
     5161 ggaagacgtg cacgcgatcg tgctcggcgc gcataccgac aaggacatgg tgcccatcac
     5221 gtcgaacgcg acgatcggca acgtgccggt cacgaagttc ctcgccgccg acaagctcaa
     5281 cgaggtcgtc gagaacacga agaagggcgg cgcgacgctc accggtctca tcggcacgag
     5341 cgcgtggctc gcaccgggcg ccggcacctg tctcatggtc gaagcgatcg tgcgcaatca
     5401 gggccgcgtt ctgccgtgct cgatcgagct caacggcgag ttcggcgtga agggcgcgtt
     5461 cctcggcgtg ccgtgcaagc tgacggcgaa gggcgccgag gcggtctacg agttcgagct
     5521 gtccgcgcag gagaaggacg ccttcggcaa ggcgctcgcc gcgaacaccg agctcatgaa
     5581 catcgcgcgc ggtctgctgt ccggcaacac gccgggctga acgcctacac gcagcgtaag
     5641 tccagctcac gatgagacgg gcttacgcag cggttcattc gcagtagata aatcgacggc
     5701 aactaacgtt cgcagctggc ggtaggccgc gaaacatcgc ggttcgacca ccggccagtg
     5761 gtgaacgact gacggatcgc tcggagtccg gcggttgcac ttctctccgc tcgtgaggga
     5821 agcgatgcat tcgctgaata cgctcgttgg ttcgatcgtc gaggaccggt acgagatcca
     5881 agaggagctc ggcgccggtg cgatgggtgt ggtgtacagg ggccgccatc tcaaggtggg
     5941 ccgcaccgtc gcgatcaagg tgcttcacga tcacctcgtg aaggatccat cgatggtcga
     6001 gcgcttcgag gacgaggcac gcgcggtggc tcgtctccgt cacagaaacc tcgtgggcgt
     6061 gctcgatgtc ggtgtcacgg acgacggtca gcgctgcatg gtgctggaat tcgcgccggg
     6121 atgctcgatg gcgaagctcg tgacggtggc gatgaagcgt gagcgggtga tcgcgcttgt
     6181 tcgtcagctg ctgctcgggc tcgagcacgc ccatgaggcc ggtctggtcc atcgcgatct
     6241 caagccggag aatgtgctcg tcgagcggag cgccgacggg acggagattc cgcgcatcgt
     6301 cgacttcggc atcgcgcagc tgcgcgaccg cgcggatcga ggagacggtc gccgccatac
     6361 gacggacggt ctcgtggtcg gcacgccgat gtacatgtcg ccggagcagg ctcgcggtga
     6421 ggctgtcgac gagcgcaccg acctcttcgc gctcggcgtc atggtgtacg agatgctggc
     6481 gggctgcacg ccgttcgaag gctcgggcgt cgaggtgatg atgctcaaca tcatgcagga
     6541 cccgccgtcg atcgcgacgc ggacgggacg cgacgtcgac gcgcagctcg aagcgttcgc
     6601 gcgcaagctg atggctcgcg acaaggcgga tcgctacgcc tcggccaccg acgcgctcgc
     6661 ggccctcgac gcgatcgcga gcaacgcggt gccgcgccct gtcgtcgaca acccgacggt
     6721 gcgccaccgt acgacgcgca tcacgatgcc cgcgctgaag cgttgaggcg cccctaacgg
     6781 aaggcgacta acggaaggac gactaacgct agacgtcctc accagcaaca cacgccatgt
     6841 ggactgcagg ccgcaggagg aatcgcggta gcttgcgcgc gcacactgcg tgcagaggtc
     6901 gggcgcatga tcggcagcat cgtcgaagat cgctatctcg tggaggagga gctgggtact
     6961 ggcgcgatgg gccgggtatt cgcagcgcgc cacgtaaaag tcggacgccg cgtcgcgatc
     7021 aagatcatgc acgacgagct cgcgaagaac gccgcgatcg tagagcgctt cgcgcgcgag
     7081 gcgatggtcg cagcacgcct gcgccacccc aatctcgtga gcgtgctcga tgtgggtcag
     7141 acggcgatgc accagccgct gctcgtgctc gagctcgcac ccggcattcc gctatcccac
     7201 atgacatgcg agccgctgca cggtccgcgc gtcgtcaatc tcatccgcca gctgctgcac
     7261 ggtctctcgc acgcgcacac ggctggtctc atccatcgcg atctcaagcc cgacaacatt
     7321 ctggtcgaaa cgaccgccga cggcctcgag atcccgcgca tcgtcgactt cggtatcgcc
     7381 gtcgccgccg atcgcgacga tacggtcgct ggccgccgcc tcaccgacgc gaacatggtg
     7441 atcggcacgc cgttctacat gtcgccggaa caagcgcgcg cgaaggacat cgatcaccgt
     7501 acggacctct tctcgctcgg cgtgatcatg tacgagctgc tcgccggaat gccgccattc
     7561 ccgggctcga gcctcgacgt ggcactcgcg aacgtgtcga aggacgcacc gacgatcgcc
     7621 gagcgcgcgg gcatcgacgt cgacccgctg ctcgaggcat tcatgcgcaa gctcatggcg
     7681 cgccgtctcg agacgcgctt ctcctcggcg cgcgaagcgc tcgacgtgct cgccctgatc
     7741 gagatcgacc gcatggcggc cgcgcgcgtg ctcggtggct cgccgctcgt cgaggtgcca
     7801 cgaccgatgc tcgcgcaggg ctccgcgccg tacgcgattc ccacgccccc caacagcgac
     7861 gagctgacga cgatcaccga gccgccggtc gcctccgacg aaagcacgcc aacggagcat
     7921 gtagacccta cggatgacga cttctcggac ctcgagttcg atcccgcgcg cagccaacgc
     7981 cgtcgcaagc gtctcgcgct cggggtggtg ttcgccgcgc tgaccgccgt cgtcgcggtg
     8041 ttcgcgttgc aggcgccgcc gcgcgtcgat gcgcctgctg tcgatgtcgc gtcgcaagac
     8101 gatgcgtcga tggcgattcg ccccgtccag acgaaggctc cgctactacc gacgacctca
     8161 gtggccgtcg tgaagaagca gcacgtcgca cgcccgacca cgaagccagt gaccgctcca
     8221 aacgtcgtcg cgccgccgcc gatgatggtc gccgcgccaa tgattgtcgc cgcaccgcgc
     8281 gcgccgacga tggtgctcgc agcagcgccg gtcattgcgc caacggccgc gccggcggca
     8341 gctccacccg ttgcgatcga ttcggtcgat gcgctcgtcg cgaagtatca ggccgtcgcc
     8401 aagaagctca agcacagcgc tgccgggccg cagacggagg atctgtggat gcgcttccgc
     8461 ttgattcgca tcaacgagtc catcgcgacg cagcaaaccc gtcgtcaagc gctcgcgctg
     8521 ctcggcgcga tcgacaacgc catcgagtag cccactcagg cgagcccgga attccgatca
     8581 gggtgacggc tggaaggaca acgctgtgga tgtcgggctg atcatcggga cgttgaggct
     8641 gatcatgcgt gccgtcgcgt tgctgccgcc ggcggtttgc atcccgatcg tagcgccgcc
     8701 tccggcaacg ctgccgctat agagatggta ttgaaccgtg atcaggccgg tggactcgaa
     8761 caacgtgatc gtcgtctcga cgacggcact ctcggggaag ttcgtcgtgc agcgatggtt
     8821 aagcgagaac gcacgcccgg ggctcgtgcc atgcgtgtgg tactgaaacc aaccgccgtt
     8881 gttgttgttg ttgaggtccc agagatacgc aacgaatgga tcggtcgaca gcgagcttgg
     8941 taacgcagtg ttcgagctcg acagagcgca tccggtaccg agaaacagca ttccgtatgg
     9001 cgaaacgctg atagtggacg tcgatgcgcc gaaccccgtg tacgtgaatc ctaaagggat
     9061 gttcgtgcac gccgttggat tcgagacgac cgtcggagtc gtgtacggca actgctcgct
     9121 gacagcgctc agagtgaacg tgatcgggct cgagccgccc gctggaccct gcggccccac
     9181 gggaccttga atgccttgcg gtccttgcgg ccccatcgca ccgtctgcac cggcaggacc
     9241 ttgcggacct tgcgcgcctt gtgagcccgt cgctccggtt gcgccttgcg gaccagcgga
     9301 accttgtgga ccagcgggac cttgtggacc ggcgggacct tgcggaccgg caggaccttg
     9361 cgggccgggc gatccttcga tgcccatcgt gcccatctcc cccatgtccc ctttggggcc
     9421 gtcaccgtta caagcgccga gcaagccgat cgttaccact acatgtacag cagttcgcac
     9481 gacgccatct caacacaggt cgtgaagcga ccgaaacagc attcgtttgc aaacgcgctc
     9541 gtgccgagct cgtgtctcca cgcgtccgct tcacttcgcg gcggcggcga tcagcgcttc
     9601 gcgatcacgc gcggggacga ggtcgcgcca ccagggcgtc gggccttcga tgtggtcggg
     9661 ctcgaacacc tggccgaggc gcggcgtcag aatgcgggcg tcgtgcttcg gcgcgagcgc
     9721 catgagctct tcacccggct cgttccaggc gtggaagccg agcgagaacg tgccccagtg
     9781 gactgggagg agcgtgccac cgccgagcat cttgaatgct tcgagcgcgt tcgctgggcc
     9841 gaggtggatg gtgccccacg atgggtggaa cgcaccgatc tcgagcatca cgagatcgaa
     9901 tgggccgtat tggcggccgg tctcgatgaa ttctttcgtc aggcccgtgt cgccggagaa
     9961 gaagatcttg tggcgctcgc tctcgatcac ccatgcggcc cacagcgtct tgttgcggcc
    10021 accgatcgag cgacctgaga agtgctgcga cggggtggcc gtgaagcgga tgccgccgac
    10081 ggtcgcggac tcgtgccagt cgagctcggt gatgcgcgac gcggcaacgc cgtacttctc
    10141 gagatagcga ccgacgccga gcgccgtgac gatcgggacg tcgagcttgg cgatctcgcg
    10201 catcgtcggt gcacagaggt gatcgaagtg atcgtgcgag aggaggatga cgtcgagctt
    10261 gggcagctcc gagagctggg ctggtgtcgg gtggaagcgg cgcgggccca cgagcgacgg
    10321 cgacggcccg atgcgattcg cgaagacggg atcggtgagc acgcggcgac cgtcgagctc
    10381 gagcaggacc gtggagtggc cgagccaggt cacgcgctgg ccggtctggg
//
LOCUS       DATLBZ010000289         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8358023, whole genome shotgun sequence.
ACCESSION   DATLBZ010000289 DATLBZ010000000
VERSION     DATLBZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8358023"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VMZ53_15060"
     CDS             <1..519
                     /locus_tag="VMZ53_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29822.1"
                     /translation="EQQAGWKHNWGPPHDRAWVEKSLAGCGHHTILLRVAGSNSDLLF
                     GLEAGMHRFIGLAGEPCHVTVELVEPQAEFTDAEWGVLGIPGAPPTARGKPMREVTIP
                     NDRVVIVGDECEAPWSELGQRLPEAAAVRYISALQHTGHHCAYDESEKLWVYENPTAA
                     LKAAEKKKKEEP"
     gene            516..>2288
                     /locus_tag="VMZ53_15065"
     CDS             516..>2288
                     /locus_tag="VMZ53_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS29823.1"
                     /translation="MRVHFTVGVYQRKDEEEEWTALVPVQYGAYISGSGESKLRDRMI
                     DRLRDVLRKAAPVHQELFQLPVGTELVRVPVDFKAKAGSMHGHLPLIVEPRWTSPERQ
                     HLFVYHPSRRDMWFVTEERDDIPALALALVREHLSDVEDEDDILGLFSEGRDRLITLA
                     FSAEPMSLLDMLPSRKKDSRTAAGTPRPDRVLHELAVNETQRISNGIVRLGVPRSPYR
                     ERMSYLLGGQRPRAVAVVGPPGAGKTMIIEQWISDRLVEDGYPIHKNLDRCFQVWRLS
                     GKRLIAGMSYLGQWEERCLSVLDEARKRKGILWIEDLHLFGRLGQSRQSERSFADFFR
                     GPVRRGDLAVVAELTQEQHARLERDAPGLAEALSLVVVPPASPQETAALLLHEVRALE
                     VKHDDISLHPFVPRTALELGTALFPWRARPGVAIEIVRRVAEEAAARSDDREIAPNDV
                     LEMLARTTGLSSRLLTLEEALDPAEVEASFAARVIGQPAATKAATDVILRVRAGLADP
                     NRPVSVLLFTGPTGTGKTELATAIAEYLYGDQSRLVRVDMGEMSGPDAVSRLIGDRWN
                     PEGMLTSRVRSQPFCVVLLDEIEKA"
BASE COUNT          395 a          767 c          750 g          376 t
ORIGIN      
        1 gagcagcagg cgggctggaa gcacaactgg ggaccaccgc atgaccgcgc gtgggtcgag
       61 aagagcctcg ccggttgcgg ccatcacacg atcctcctgc gcgtcgcggg ctcgaacagc
      121 gatctgctct tcggcctcga agcgggcatg catcgcttca tcggcctcgc gggtgagccg
      181 tgccacgtaa cggtcgagct cgtcgagccg caagccgaat tcaccgacgc agaatggggc
      241 gtgctcggga ttccgggcgc accaccgacg gctcgcggca agccgatgcg cgaggtgaca
      301 attccaaacg atcgcgtcgt gatcgtcggc gacgaatgcg aagcgccgtg gagcgagctc
      361 ggtcagcgcc tgcccgaagc ggccgccgtg cgttacatct ccgcgctcca gcatacgggt
      421 caccactgcg cgtacgacga gagcgagaag ctgtgggtct atgaaaaccc gaccgctgcg
      481 ctcaaggccg ccgagaagaa gaagaaggag gagccgtgag ggtccacttc accgtcggcg
      541 tgtaccagcg caaggatgaa gaggaagagt ggactgccct cgtgccggtg caatacggcg
      601 cgtacatctc gggtagcggc gagtcgaagc tccgcgaccg catgatcgat cgcctgcgcg
      661 atgtgctgcg aaaagctgcg cccgtgcatc aagagctgtt tcagctcccc gtcggcacgg
      721 agctcgtgcg cgtgccggtc gatttcaagg cgaaggccgg cagcatgcac ggtcatctgc
      781 cgctgatcgt cgagccgcgc tggacgagcc ccgagcggca gcacctgttc gtctatcacc
      841 cgtcgcgtcg cgacatgtgg ttcgtcaccg aggagcgcga cgacatcccc gcgctcgcac
      901 ttgcactggt gcgcgagcac ctcagcgacg tcgaggacga agacgacatc ctcggcttgt
      961 tcagcgaggg tcgcgatcgg ctgatcaccc tcgcgttctc cgccgagccg atgtcgctgc
     1021 tcgacatgct gccgtcgcgc aagaaggaca gccgcaccgc tgccggcacg ccgcgtcccg
     1081 atcgcgtgct gcacgagctc gccgtcaacg agacgcagcg catctcgaac ggaatcgttc
     1141 ggctcggtgt cccgcggtct ccgtatcgcg agcgcatgag ctatttgctc ggcgggcaac
     1201 gtcctcgcgc ggtggctgtc gtcggcccgc ccggtgcggg caagacgatg atcatcgagc
     1261 agtggatctc ggatcgcctc gtcgaggacg gctatccgat ccacaagaac ctcgatcggt
     1321 gctttcaagt ctggcgactg tccggcaagc gcctgatcgc gggcatgtcg tatctcgggc
     1381 aatgggaaga gcgctgtctc tccgttctcg acgaggcgcg caagcgcaag ggcatcctct
     1441 ggatcgagga cctgcatctg ttcggcaggc tcggccaatc gcggcagagc gagcgcagct
     1501 tcgccgattt cttccgcggc cccgtgcgtc gaggcgacct cgcggtcgtc gcagagctca
     1561 cgcaagagca acacgctcgg ctcgaacgcg atgcgccggg tcttgccgaa gcgctgtcgc
     1621 tcgtcgtcgt gccgcccgca tctccgcagg agaccgccgc gctgctgctc cacgaagtgc
     1681 gcgcgctcga ggtcaagcac gacgatatct cgctgcaccc gttcgtaccg cgcaccgcac
     1741 tcgagctcgg caccgcgctg tttccgtggc gcgcacgccc cggtgttgcg atcgagatcg
     1801 tacgtcgcgt cgccgaagaa gcagcggctc gcagcgatga ccgcgagatt gcgccgaacg
     1861 atgtgctcga gatgctcgcg cgcacgaccg gcctgtcgtc gcgccttctc acactcgaag
     1921 aagcgctcga tccagcggag gtcgaggcgt cgtttgcagc gcgcgtcatc gggcagcccg
     1981 cagcgacgaa ggcggcaacc gatgtgatcc tgcgcgtgcg cgccggcctc gccgatccga
     2041 atcgtccggt ttccgtactg ctcttcaccg gtccgacggg cacaggaaag accgagctcg
     2101 cgaccgccat cgctgaatat ctgtacggcg atcaatcgcg gctcgtacgc gtcgacatgg
     2161 gcgagatgtc cggccccgac gccgtctcgc gcctcatcgg cgatcgctgg aatccagaag
     2221 gcatgctcac gtcgcgcgtg cgcagccagc cgttctgcgt cgtcctcctc gacgagatcg
     2281 agaaggcg
//
LOCUS       DATLBZ010000290         6259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9853311, whole genome shotgun sequence.
ACCESSION   DATLBZ010000290 DATLBZ010000000
VERSION     DATLBZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9853311"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VMZ53_15070"
     CDS             <1..386
                     /locus_tag="VMZ53_15070"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUS29824.1"
                     /translation="ISVVPDKTRVFRIDEAWITMDAGTVFNKERVHAQMEGSIVMGIS
                     NALYGGISQTNGVTDQTNFRDARIARIRDIPQTIHTDLIASTAPPSGVGEPGVPPVGP
                     ALANALFALTGKRLREFPLAAALGV"
     gene            complement(598..3048)
                     /locus_tag="VMZ53_15075"
     CDS             complement(598..3048)
                     /locus_tag="VMZ53_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUS29825.1"
                     /translation="MTACSTADAPDDPWGGDGKGDGWATERHVEVLFNEPFCDVCTAA
                     DKTIMLDRSPMTKRLVALMDGAQTSIDIANFTFSVKPLEEAILRAKMRGVAVRVAMDK
                     GQETADTVATRLKAAGIDVRFVAGQSGDPVGLQHAKFMIVDKLTLASGSNNWSSTGTS
                     INEESTIVTKSVDGDPLLAGFACHFTAIWGAKPGDAGACSNTEVAFAPSSKPIALIKE
                     EIARGQKSIDVLMHHFVFDDLVTELAKAAERGVRVRVIINAADRAEATGAKWNRLFAA
                     GGQLRYKQTNADLFQLMHHKLMVVDDRVVVNGSGNWSGSAFFKNFENYVRYRDPRIAR
                     PYSELFDRLWLWSLSGASLDAGKTAAQQHAEETKIFFGNLHAHFAANAQGAALDDGKA
                     IRADASGTMQPVDVGMTPSDAARYAYEYARDEGKMDFLALSPHTADDAPAESGDPANM
                     IPQQFAQLRLVAEDVTQQSTGSFVAIPAMEWSTNSSGNHVNIFGSKELAKLERGRFDL
                     LFDDFLVGREHDGDHPLVQFNHPRTFRQQTVSLDGNWDQIFDTKLTDIPSQADREKKF
                     NDFGLDDYEPMKSALPTWISGTAMPSHDVVNQTLSAVELAGRPYVRLFEVLVGRGTSI
                     AGETHENSSLMLDSTTNTISRYTRVHSDWDYYLLHGFKTAPTAPHDNHFANWGTGHST
                     RTAIIAEKLTEDSLLEAIDHRAAYASEDEELQLRVYANKRIPMGRSLTTHDATIQLDV
                     QLSDADYTGSFEVVVYGGAIGGDAVHEVKRQTITSGSWQSITVDVPSLGQQFFYLEIK
                     EPSPDRMAWSAPIWVERI"
     gene            complement(3173..3691)
                     /locus_tag="VMZ53_15080"
     CDS             complement(3173..3691)
                     /locus_tag="VMZ53_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29826.1"
                     /translation="MRAASLLLLFVACGGDDGATMRDAAIDSAPQFSMTCTGACQTTT
                     LTAMMSATRVLDRAYFGITAADNTLHVEAYKGGAAGCPTMSSPTPQYTLVLGRVTRPT
                     STTTSSSPGNMLDYQGDLLGGGLGQAATSVTLTPVAAMGDAFVAFDVMLTFPSGTVTG
                     HFYATHCASLDG"
     gene            3863..4234
                     /locus_tag="VMZ53_15085"
     CDS             3863..4234
                     /locus_tag="VMZ53_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29827.1"
                     /translation="MKLGILSCVAVLSLSSIAMADVRVDLNAGATTLSECGGTIEATA
                     RGSSPNVNLVLKNVEKCSNFVLQNTKKVYKVPGADGDRAGSFSISAGKLHNGWNQVKL
                     TVRSNSGKTRDDISIWVQVAR"
     gene            complement(4231..5661)
                     /locus_tag="VMZ53_15090"
     CDS             complement(4231..5661)
                     /locus_tag="VMZ53_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS29828.1"
                     /translation="MRSRLFVLVFLAACGDPLTTDLGEGGFGDSGGTSGGGIDPGGAQ
                     DIGAAREIINSGGVPSGSLITVEGLLSEHDVPTIGAPCQSLICLRPATGVAPSLETGK
                     LSRWVHIGMTTALDPATFQRPPVDLVVAIDKSSSMSIDMAQTTEAVSRLVGHMRADDR
                     IAILAFDENVHIIHELGTVADVPALQTQIKAVHASGSWDINMAMNAAYGRLAEPSEGR
                     IRHVVVLSCGYPGVSPTADDPTSMLIRDRGTAGVGITFVGVLLGWDPNLAALLGKTNG
                     ANYYYLGDLNRVQEVFDKDADLIIAPIAYDLSMQLSLPQGWKLARMYGIPGDKDGTPA
                     SGYTIATGFLSRKRGSIVARLEWGGTGSAPATMGTLQLSYRPEPALGWTAAENQSAAA
                     ESGTPEADGTYFAGPGVHKATMLVNMAVQMIEGCNKYPSNAADARAIIATLRDYLVDE
                     QAKLGAPDMDAEITLVNKLLTNMGGT"
     gene            complement(5860..>6259)
                     /locus_tag="VMZ53_15095"
     CDS             complement(5860..>6259)
                     /locus_tag="VMZ53_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS29829.1"
                     /translation="INPSQLPTNGGRMGIVDMNGQYGFFVHETGNLQCTMVNGVSSAP
                     VTANVATGAWSHVACTYDGTTTTIYKDGVAVFTGTGGGTLATSGGTGISIAADNPPNA
                     GSRLLGLIDEVRIMSRARTAKEICNDAGCP"
BASE COUNT         1050 a         1979 c         2065 g         1165 t
ORIGIN      
        1 tcatctccgt cgtcccggac aagacgcgcg tcttccgcat cgacgaagcc tggatcacga
       61 tggacgcggg caccgtcttc aacaaagagc gggtccacgc ccaaatggaa ggctccatcg
      121 tgatgggcat cagcaacgcc ctctacggcg gcatctccca aaccaatggc gtgaccgacc
      181 aaaccaactt ccgcgacgcg cgcatcgccc gcatccgcga catcccgcag acgatccaca
      241 ccgacctcat cgcctccacc gctccgccgt ccggcgtcgg cgagcctggc gtgccgcccg
      301 tcggcccggc gcttgcgaac gcgctgttcg ccctcaccgg caaacgcctc cgcgaattcc
      361 ccctcgccgc cgccctcggc gtctagcgct catgaccgcg cgcgcgctca tgacgcggct
      421 tgacgcgcgt cataaacgac cggggtaagt gactccggaa ccgcaagtcg ttgatgacgc
      481 gtctttgacg cgcgtcatga actaccgggg taagtgaatc cggaggcagt gtgtccggcc
      541 gccggacgaa cgcgcttctc tccgccgatg gcgattagga aattcggtcg gactcgatta
      601 aatgcgttcg acccagatcg gcgcggacca cgccatgcgg tcggggctcg gctctttgat
      661 ctcgaggtag aagaactgct ggccgaggga ggggacgtcg accgtgatcg actgccacga
      721 gccggacgtg atcgtctggc gtttcacttc gtggacggcg tcaccgccga tcgcgccgcc
      781 gtagacgacg acctcgaacg agccggtgta gtcggcgtcc gagagctgga cgtcgagctg
      841 gatcgtcgcg tcgtgcgtcg tcagggagcg gcccatcggg atccgcttgt tggcgtagac
      901 gcggagctgc agctcttcgt cttcggaggc gtacgcggcg cggtggtcga tcgcctcgag
      961 cagcgagtct tcggtcagct tctccgcgat gatcgcggtt cgcgtcgagt gaccggtgcc
     1021 ccagttggcg aagtggttgt cgtgtggggc ggtcggtgcc gttttgaagc cgtggagcag
     1081 gtagtagtcc cagtcgctat gcacgcgcgt atagcggctg atcgtgtttg tcgtcgagtc
     1141 gagcatcagc gaggagttct cgtgcgtctc gccggcgatg gatgtgccgc ggccgacgag
     1201 gacttcgaac agccgcacgt acgggcgacc ggcgagctcg acggcggaca gcgtctggtt
     1261 gacgacatcg tgcgacggca tcgcggtgcc agagatccat gtcgggaggg cggatttcat
     1321 tggctcgtag tcgtcgagac cgaagtcgtt gaacttcttt tcgcgatcgg cttgggatgg
     1381 gatgtcggtg agcttcgtgt cgaagatttg gtcccagttg ccgtcgagcg agacggtttg
     1441 ttggcggaac gtccgcgggt ggttgaactg gacgagcggg tggtcgccat cgtgctcgcg
     1501 gccgacgagg aaatcgtcga acaggaggtc gaagcggccg cgctcgagct tcgcgagctc
     1561 cttggagccg aagatgttga cgtggttgcc cgacgagttc gtgctccatt ccatcgctgg
     1621 gatggccacg aacgagccgg tggattgctg cgtgacgtct tcggcgacga ggcgcagctg
     1681 cgcgaactgc tgcgggatca tgttcgcggg gtcgcctgat tcggcgggcg cgtcatcggc
     1741 cgtgtgcggc gacagcgcga ggaagtccat cttgccttcg tcgcgggcgt actcgtacgc
     1801 gtagcgcgcg gcgtcgcttg gtgtcatgcc gacatcgacg ggctgcatcg tgcccgatgc
     1861 gtcggcgcgg atcgctttgc cgtcgtcgag cgcggcacct tgcgcgttgg ccgcgaagtg
     1921 agcgtgcagg ttgccgaaga agatcttcgt ctcctcggcg tgctgctgcg cggccgtctt
     1981 gccagcatcg agcgacgcgc ccgagagcga ccacagccac aggcgatcga acagctcgct
     2041 gtatgggcgg gcgatgcgcg ggtcgcggta gcgcacgtag ttctcgaagt tcttgaagaa
     2101 cgcactgccg ctccagttgc ccgagccatt cacgacgacg cgatcgtcga cgaccatcag
     2161 cttgtggtgc atcagctgga ataggtcggc gttcgtctgc ttgtagcgga gctggccacc
     2221 tgccgcgaac aggcgattcc acttcgcgcc cgtggcttcg gcgcggtcgg cggcgttgat
     2281 gatcacgcgc acacgcacgc cgcgctcggc ggccttggcg agctccgtca cgaggtcatc
     2341 gaacacgaag tggtgcatga gcacgtcgat ggacttctga ccacgcgcga tctcttcttt
     2401 gatcagcgcg atcggtttgc tgctgggagc gaacgcgacc tcggtgttcg agcacgcgcc
     2461 ggcatcaccc ggcttcgcgc cccagatcgc ggtgaagtga cacgcgaagc ccgcgaggag
     2521 tgggtcgcca tcgacggact tcgtgacaat cgtgctctct tcgttgatcg acgtaccggt
     2581 cgatgaccag ttgttgcttc cgctcgcgag cgtaagcttg tcgacgatca tgaacttcgc
     2641 gtgctgcaag ccgaccggat cgccgctctg acccgcgacg aatcgcacgt cgatgcctgc
     2701 cgctttgagc cgcgtcgcga cggtgtcggc ggtctcttgc cctttgtcca tcgcgacgcg
     2761 aacggcgacg cctcgcattt tcgcgcgcag gattgcttct tcgagcggct tcacgctgaa
     2821 cgtgaagttc gcaatatcga tcgacgtctg cgcgccatcc atcagcgcga caagccgctt
     2881 cgtcatcggc gagcggtcga gcatgatcgt cttgtcggcc gcggtgcaga cgtcgcagaa
     2941 aggctcgttg aacaggacct cgacgtgccg ctcggtggcc cagccgtcgc ctttgccgtc
     3001 accgccccac gggtcgtcgg gagcgtctgc agtgctgcag gcggtcatgc aaacgaaaag
     3061 cagcagtggc gacacggctt ttcggcgcat acgcgcgttt tgtgcaccgt gcatgccctc
     3121 gtaacccagc gatcacaggc gtccgagggg cggccagtcg acctgccggt ggctagccat
     3181 ctagcgatgc acagtgcgtc gcatagaagt gaccggtcac ggtgcccgac gggaacgtca
     3241 gcatcacatc gaacgccacg aacgcatcac ccatcgccgc gaccggcgta agcgtcaccg
     3301 acgtcgcagc ctggccgagg ccgccgccga ggaggtcgcc ttgatagtcg agcatgttgc
     3361 cgggtgagct cgacgtcgtc gtcgatgtcg gacgcgtgac gcgaccgagc acgagcgtgt
     3421 attgcggcgt cggcgagctc atcgtcggac agcccgcagc gccgcccttg tacgcctcga
     3481 cgtgcagcgt gttatcggcc gccgtgatcc cgaagtacgc acgatcgagc acacgcgtcg
     3541 ccgacatcat cgcggtgagc gtcgtcgtct gacacgcgcc ggtgcacgtc atcgagaatt
     3601 gcggcgcgct atcgatcgct gcgtcacgca tcgtcgcgcc atcatcacca ccgcacgcga
     3661 cgaacaacag caacaacgaa gcagcgcgca tgtcgcattc tattctggct gggggtggag
     3721 ccggaggaaa gagtcgccgg caactgcacg ctccgctttc ggctcacatc gcgccagggg
     3781 cagcgtcgat tgtgacaacg cgatgaaaac cgcacggcac gcgcgttgct cgttgggttg
     3841 gcaactcaac aaggagatcg ccatgaagct cgggattctt tcgtgtgtcg ctgtgttgtc
     3901 gctctcgtcg atcgcgatgg cggatgtacg cgtcgatctc aacgcaggtg ccacgacgct
     3961 cagcgaatgc ggcggcacga tcgaagcgac cgcgcgcggc tcatctccga acgtcaacct
     4021 cgtgctcaag aacgtcgaga agtgctcgaa cttcgtcctg cagaacacga agaaggtcta
     4081 caaggtaccg ggcgccgacg gtgaccgcgc cggcagcttc tcgatctcgg cgggcaagct
     4141 ccacaacggt tggaaccaag taaagctcac ggttcgctcg aactccggca agacgcgcga
     4201 cgacatctcg atctgggtac aagtcgcgcg ctaggtgccg cccatgttcg tcagcagctt
     4261 gttgacgagc gtgatctccg cgtccatgtc aggcgcgccg agcttcgcct gttcatcgac
     4321 gaggtaatcg cgcagcgtcg cgatgatcgc gcgggcgtcc gctgcgttgc tcgggtattt
     4381 gttgcagccc tcgatcatct gcacggccat gttcacgagc atcgtcgcct tgtgaacgcc
     4441 gggaccggcg aagtacgtgc cgtcggcttc gggcgtgccg gactctgcag cagcagactg
     4501 gttctccgca gcggtccagc cgagcgccgg ctcggggcga tagctgagct gaagcgttcc
     4561 catcgtcgcc ggtgcgctgc cggtgccgcc ccactcgagg cgcgcgacga tcgaaccgcg
     4621 tttgcggctc aagaatcctg tcgcgatcgt gtagccgctc gcgggcgtgc cgtccttgtc
     4681 gccggggatg ccgtacatgc gcgcgagctt ccaaccttgc ggcagtgaca gttgcatcga
     4741 gaggtcgtat gcgatcggcg cgatgatgag atcagcgtct ttatcgaaca cttcctgcac
     4801 gcgattgaga tcgccgaggt agtagtaatt ggcgccgttc gtcttgccga gcaacgcggc
     4861 gaggttcgga tcccaaccga gcagtacgcc gacaaacgtg atgccgacgc cggcggtacc
     4921 gcgatcgcga atcagcatgc tcgtcggatc gtcggcggtt ggcgagacac ctggatagcc
     4981 gcacgacagc acgacgacgt gccggatgcg tccctcgctc ggctccgcca ggcggccgta
     5041 cgcggcattc atcgccatgt tgatgtccca cgagccactc gcgtggaccg ctttgatctg
     5101 cgtctgcaac gccggaacat cggcgaccgt accgagctcg tgaatgatgt ggacgttctc
     5161 gtcgaacgcg aggatcgcga tccggtcgtc tgctcgcatg tgcccgacga gccgcgacac
     5221 cgcctcggtc gtctgcgcca tatcgatgga catcgagctc gacttatcga tcgcgacaac
     5281 gagatccacg ggcgggcgtt ggaacgtcgc gggatcgaga gcggtcgtca tgccgatgtg
     5341 gacccagcgc gagagcttgc ccgtctcgag tgacggcgcg acgcccgtcg cgggcctgag
     5401 gcaaatgagc gactggcacg gtgcgccgat cgtcgggacg tcgtgctcgg agagcagacc
     5461 ctcgaccgtg atcaggctgc cgctcggcac gccgccgctg ttgatgatct cgcgcgctgc
     5521 gccgatgtcc tgcgcgccgc cgggatcgat gccgccgccg ctggtcccgc cactgtcgcc
     5581 aaacccgcct tcgccgagat cagttgtgag gggatcgccg cacgcggcga ggaagacgag
     5641 gacgaaaaga cggcttcgca tggagcgatg gacttcgaag gccgtgccac cgacgcgcga
     5701 gcgatctcgc atggttgagc gtcacgcgtt ccggaaagcc gcctcgcccc acgacatccg
     5761 cgtcagtttt ttgagagggg tcaggcacaa gtaaacaagt aacgcggctt cctccacgga
     5821 gagcgcgccg agtttcttgt gaacttgtgc ctgaccccgt tatgggcagc cggcgtcgtt
     5881 gcagatctct ttggctgttc gggcgcgcga catgatgcgg acctcgtcga tgaggccgag
     5941 caggcgcgag cctgcgttcg gtgggttgtc cgcggcgata gagatgcccg tgccaccgct
     6001 cgtcgcgagc gttccgccgc ccgtgccggt gaagacggcg acgccgtcct tgtagatcgt
     6061 cgtcgtcgtg ccgtcgtagg tgcacgccac atgagaccac gcaccggtcg cgacgttcgc
     6121 agtcacgggc gcgctgctga cgccgttgac catcgtgcac tggagatttc cggtctcgtg
     6181 cacgaagaag ccgtactggc cgttcatgtc gacgatgccc atacggccgc cgttcgtcgg
     6241 cagttgcgac ggattgatc
//
LOCUS       DATLBZ010000291        17948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6123756, whole genome shotgun sequence.
ACCESSION   DATLBZ010000291 DATLBZ010000000
VERSION     DATLBZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17948
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6123756"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VMZ53_15100"
     CDS             <1..1128
                     /locus_tag="VMZ53_15100"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS29830.1"
                     /translation="VPLELGASLPFSIVDGSVDGQGIGDFGFHAKARVARVGRVGVGA
                     IASLYVPTGSDRNMFVGEAATTPQLMAVADANLGRLRLAVNGGVRLRRTTTMGSVTSA
                     TTVPVGIAAAYALSPEKVELVAEVFGSLPTGDGYRSAEALGGLKVYLAKNSYMSLGAG
                     RGLLPGEAGNPDFRAVIGIVFEPKPAQRVSHSIADDVVAEMTPPKKAGDLDADSDNDL
                     IRDRDDRCPDDMEDYDGEEDEDGCPEASRERIVDVGSELVTLQPIEFEFDKDVLRDSA
                     FPILDEVVKAFIDNSDIELVEVQGHTDEQGNDAYNLDLSKRRAATVMRYLDDHGIAAS
                     RLTSNGYGETKPVDKRHTQDAYKANRRVQFVIQKRGGNLMP"
     gene            1125..2978
                     /locus_tag="VMZ53_15105"
     CDS             1125..2978
                     /locus_tag="VMZ53_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS29831.1"
                     /translation="MIRPALLLLVFTASARAQELTDEELLKMSEEETIEVYDERPDKP
                     FDRDTEVRLTGEELAARGAVDLATALDLLPDVDVRDGGRGGFNVDIRGARKGAVSVLI
                     DGVLVSDPYYGTFDVSTIPITDIVQIRMSTSPQSPIDGPGGPGGVIEVLTRDAIGPQL
                     VLVRTTGDTLPSFGLAGSARAGLAKNLALRLSASGLAGAREFDAKGGGLDENRHAATG
                     AARVEYRDDTRRLVVDGFLDDRHYISPPSETSSILVIDRETTERVSAKGDVQRGKLQV
                     QGQAFVSHLHRISRNFMDVNLTVQQRVENLLALRTGAGALVTKPINQEMRWTASASIA
                     RDSGRVAGGNNGPVEGHVTTLEAAGDFQYERGPARIDAAVGAAVPFGIDANPWPEAKL
                     VAKWRPSYGPVELTATGARKGRVPSLRERFDPSQGGNDGLDPEFATHAELRAVAEKKD
                     VYRVELAPFFRRTNGTIRRSTDPADMGMLANLDRLDAKGFDLIARFTPHPRVTVGGAF
                     DYITLSGGMLDRLPQRRAEAWIQGTPEKRISLLARVRYYGAFYDQAPSGATEPNLPAY
                     TTVEASATATLSKQYLAVLRVDDLLDVRPETHLGYFGPGRVVMLVLQGQWE"
     gene            3127..4563
                     /locus_tag="VMZ53_15110"
     CDS             3127..4563
                     /locus_tag="VMZ53_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS29832.1"
                     /translation="MRSWSGLVVCGFLLAACGGDSGPGGGGSFGATAGGVKDMRYARD
                     LISKGVIPPPDAILVEAMFSEHDLALDGPPCARTLCLRSAAGFADSRGFVQVGLSSTI
                     DPETWERPPTTFIFTVDVSGSMGWGYGDGATPGELSRALLHTLTEQLREDDQVAIVTY
                     GDVVSTKLAITSGAAKPAIHQIIGALQTEGVTAMEKGMQRAYEVAANAPASTPNVRVI
                     VFTDTQPNVGATSPESFNAMVAAAADGGVYTTVLGLGVGMAPDVFRAMASLRGANAFS
                     LTSQVHIGEWFLDEWPWFTTPIAFDLRVNASLSPGWAIERGFGFPAATDAQQVGLKAS
                     SVFLSKRRGALLVELDSPNGTPAGLDGTFSLAYVDANGSQVTETTPFAYDGSALARGQ
                     WFAQHGVARATALALLTDAMHQAAIDYAAHPDFAEVTMRLAYDRFTADAAQLGDADLA
                     QEVDFARSLLTLIEERAEQGALYGDYAF"
     gene            complement(4551..5405)
                     /locus_tag="VMZ53_15115"
     CDS             complement(4551..5405)
                     /locus_tag="VMZ53_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29833.1"
                     /translation="MAKPKAKAAKKKPVAKKKPSAKKPSAKKSVAKKSVAKKVAPKRP
                     IGASAGHSGELLGEITCPSGTLDVFDVGLLGYLGRDALEPAIVKAQVPSDRALAVIGH
                     RVGAGKEYGDCWDRVIVKLASGVPTHWKKLGEASVDFARLVVMDHSALDVWTHEDTLD
                     GLADVVFWGRDAAAAARASGAKSMKEGYGWTNLSLDVAEEKALEVDKLKHANKWAMNI
                     DFRPHSHHFYALAEARKNKLGAGTIEVGDAKVCLFFTSWGDGVFPVFLDLDEQDRPLQ
                     VRVQLQKA"
     gene            complement(5415..7064)
                     /locus_tag="VMZ53_15120"
     CDS             complement(5415..7064)
                     /locus_tag="VMZ53_15120"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29834.1"
                     /translation="MRNLLLASTLTLLVTSACNDEVGALPEPPVLKITSPQRSLIRDH
                     AGSIEVTGTVQPSVDGSPVKKVMVNGVAATVNTDGTFTAQLNLAAGATLITTTAEAKN
                     GGKATDTRSVEAGQLRAPGSNIENAVTTAISKEAFAKIAGAGGTMLKAMDFKPMLTPM
                     NPIAHTGDEGGEDCLFARGYVDDFKMSNATIQLVPVVGGLSMSIKLDGVDVPGHARYA
                     AACISGSNNLRVQASSVLVKGTLMITPDGMAGFKTDLKNETVQITGLNISASGIPGTI
                     LDMLPLESLIEKAAPLAAKLFMGPMMNKALGGLGGPKELNVLGKTISMQVSPSDIMFD
                     AEGGLITLDMKMLIKGTESSKGFIFTDNGIPTMDPGSGLMIGLADDLANSMVSQLVAT
                     GMLNLSMPAQGGTFNKTEIAMTSPPMISADPVDGKMKLVLPDMMATFTLDGSPVGRAA
                     INATVDLKITPALNGYGVAIELGKPEIHANTVEDEIQNHTMMTDEDLGKAVELCLDSQ
                     ISSISALLGSIPLPAMPAGIVMTNMSVTSDDGYVMVKGTLQ"
     gene            complement(7295..7642)
                     /locus_tag="VMZ53_15125"
     CDS             complement(7295..7642)
                     /locus_tag="VMZ53_15125"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HUS29835.1"
                     /translation="MSTKRDSPFSQLEHLRDQLPKGVQQTVAPVPDAPKPGPKRAVVR
                     YERKQRGGKEVTVVEKLDLKAAELEQWCKELKQALGCGGFVEETTIVLQGDLRKRLPD
                     LLTKKGVAKVTVS"
     gene            7731..9902
                     /locus_tag="VMZ53_15130"
     CDS             7731..9902
                     /locus_tag="VMZ53_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29836.1"
                     /translation="MGVTANREAVVKTGDMVEGRYRIIKTLGAGGMGTVYLAEHALIK
                     RRVALKVLHPELAADSNVVERFMNEARAAGTLGHPNIVESTDMGFTHDQVPYIVFEYL
                     EGTLLTDEIYRVGGLPVRRAVRIADQMASALSAAHNANIIHRDLKSDNIFLTDKDDAL
                     DHVKVLDFGVSRFLEAEERQRGLVVGTPEFMAPEQITSPDHVDTRADIYALGVIMYEM
                     LTARRPFSNDDDPRQLMHRIVHESPPPLQRAEVPHALSTMILERMMAKSPAHRFQTMA
                     DVQSALEQFTTGGLASQPRRRSAPIAAQTMAFEDDQPETSIRLPKPAPVRNTPWPVDT
                     LDTPIGVGQVSLPMPPPVKRPYALYGVAGAGILMGIIGLVVGMRGGKEQAPQPVAQQQ
                     QQMQQPQQLPAAATQAAPEKIEVKLSADVPNARVVFRRRVADAPVQMEINSTDVVELV
                     EVSAPGYKTTRYWLTFDRATYLTAHLVKGTGSVEATEEDTLVALGEVIMVKDTAPAPA
                     AAPVQVAMAEKPVEAVVEAAKPVEAKAVVAVVAPTVAPRKIGRSAASDEVTEATTAPP
                     AETKAEEPKPVETKPLETKPAEAQPAIAKTETVLPKEEPVQQPKQEPVKPVEQPKEVV
                     AEPVRPAIDKATVTSVISQHRPEVLKCFSEGKKKNAQMKGTLGLQLQVEPSGKVFRVQ
                     VQSTLNAPLVAACVVKAANGWKFPTRSGGDVATVEYPFTIN"
     gene            9937..12081
                     /locus_tag="VMZ53_15135"
     CDS             9937..12081
                     /locus_tag="VMZ53_15135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HUS29837.1"
                     /translation="MRALLVFGVSFLVACGDDATPLPEVTDSTEHVDPRIGTGGLGFA
                     HGSCFVGALVPHGLAKPGPDTSGPFGVVSFQHYSGYFAEDNKIRGFSQLHLHGTGATD
                     YGVLSLMPTLAFDTAKQKVTDYETLFAKEDERAEAGYYGVKLANGIDVELTATTRVAV
                     HRYTLPSAGAVVVDLVKVLDGGMVDASEIHVATDEVYGSLHHKGGMSGGFGGYTLYFT
                     MRGTVPFSAAAEWTGGAALSLPAGASTIAIGLSFVSLDGARANLAAEVPTVDFDGVRA
                     TARDAWRAKLDVVKLTGGTAEQRRTFYTSFYHAFMMPTVIDDHSGDYVLAYSPVVQHH
                     EAGWSMVSDMSLWDTYRTTVPLYSWLSPQSARDQVKSLEGFAAQLGFYPKWPVAIGES
                     GTMLGSSAEIAIADTIVRGGQTDLGVNMTMLLTSTGGGRDKVADYTQYGYVPRASGNR
                     TASLTVEYAHDDYALAQLTGDAALLERSHGWRKLYDPAVGFVRARNADGSFPAGAFDA
                     TTWIDDYAEADAWQSLFEAGVHDPDGIAEILGGTDAAVAKLTEFFEKAKTDWENSDES
                     ANNFPRRYYWAGNETDLNAAYLFAQLGRPDLTQKWSRWAIDTFFTDQPSGVPGNDDGG
                     TMGAWYVFATLGLYPVAGGDRYVIAAPRFPKARIAIGGHELTIVADGVSESAMYVSSV
                     DLDGVPVDMPYLTQAQLNSASTLHFVMTSKAP"
     gene            12131..12556
                     /locus_tag="VMZ53_15140"
     CDS             12131..12556
                     /locus_tag="VMZ53_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29838.1"
                     /translation="MSRLVLLFGFAVAACVAQASPQPTSPGGPPPNGEYAAAPAAMPA
                     GGQVCGSGQVCNWTCDPGNCNFMCQSGATCNMTCDGGNCAIDCQGGAVCNATCDGGNC
                     QARGANGASLNGTCDGGNCAIACEHGAVCSGTCDGGNCH"
     gene            12613..13764
                     /locus_tag="VMZ53_15145"
     CDS             12613..13764
                     /locus_tag="VMZ53_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29839.1"
                     /translation="MVPSLLLATLCFACGNDERPLPPPPEVRPPVRGAVGDTDLRVML
                     AEIASSKACEIIKDSFRALKGTQDPNTMTGLLWIRECKITNAGTKVTYELAGQGWQWA
                     EQTKKKAGGTFELSEYVKFGVKAHIEGSIDIAYDTKNHIASIWYSPTEKPSVTFEPIG
                     DVDVEEKGAWSEVVGGLSTLVGSSPDRQGEKQAKKQGTNEFERQLGEGMTVAVDLCTG
                     YQRFTLGRPDKGDLGPPNVGESFTQPVELAKNGMIVFGPYFAQNGMHVKLNSDGPVRA
                     GLACADDVHAAADAFVHEQPLPQIATLAQADITGTGTLKIKPQRCKVVLVAKSLSENK
                     VRFDWARPPQEIAQSTGGPAVHCARKGTLSSASNAAGSGASPSTAARRR"
     gene            13715..14170
                     /locus_tag="VMZ53_15150"
     CDS             13715..14170
                     /locus_tag="VMZ53_15150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS29840.1"
                     /translation="MLPDLEPRLLPPLVDGEVELHLAERSLADPARNRSAQYRWEIRI
                     GISVVGNISLRLGHSEYIEKYAGHIGYGIDAEHRGHHYAARAVKLVLPIAKSHDFRVV
                     WITCNPENVASRRTCELAGGQLIDIVDLPPEIDMYKEGERQKCRYRITL"
     gene            14167..15075
                     /locus_tag="VMZ53_15155"
     CDS             14167..15075
                     /locus_tag="VMZ53_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HUS29841.1"
                     /translation="MTGRDVIAFIKLGRPMFLGGGFILYALGAVIAARHGHAIDWRHY
                     LVGQGAVTAFQLMTHYANDYFDYDADRQNATPTKWSGGSRVLPDGDLPRHVALVAALV
                     FAALGITASIALAASRAGSLAVPTLALVLVLAWEYSAPPLRLCAAGLGELTTAIVVTV
                     LVPWLGFYLQAPAGGGGRALALTIVPLALLQIAMLLAIEFPDAAGDAATGKRTLVVRL
                     GAATAARLYVAITALAYLWPIAALALGLPARVVVALAIPAPIAIWRMMRIADHADASA
                     FERLTFWAVFLLVATSALALGAWLTL"
     gene            15083..16411
                     /locus_tag="VMZ53_15160"
     CDS             15083..16411
                     /locus_tag="VMZ53_15160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS29842.1"
                     /translation="MTYDAIIVGARCAGAPLARFLARAGKKVALVDSATFPSDQPMST
                     HFIHPYGMRVLEDLGLAERIRDISPPVNLMRQDVGGHIVEFRINPGGSCPRRIDLDQV
                     LLDGAREAGAEVRLGERVIEIIREKSRVVGVVTASREATRHELRGNVVVGADGRHSTI
                     GDLVEAERYFEYVGPRGAYWAYWPRPKFFDEAPYNGATMLRFVGEHFQFVFPTNRDQL
                     ILGVGFPREELATWKTDPHAKLLEHLRSTPFFKPLVEAQPISKTLGLTKAEYFFRRAA
                     GPGWALVGDAGLFKDPTPGLGITDALRDAKNLAAAIIEGGDQALERYWRQRDVDSIEL
                     FNFARDMGELFYNNALTRMLFAKLETDPELGQRMVRVIEREISPYGAFKPSEVVRWTF
                     GALLRGKLGVLPAFFRAGFRGAQVQKELKRRRALLVPALPASPVLAEKAA"
     gene            complement(16408..16896)
                     /locus_tag="VMZ53_15165"
     CDS             complement(16408..16896)
                     /locus_tag="VMZ53_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29843.1"
                     /translation="MRKLRVAVAVMLVACDRGSAKQPPPAPAPRDATAARLDASPVDA
                     HLDDAPAVDAMVLAPPKPAALQTRDCTKIAKRHLGRTYSNEERREYDEIIRKRCVETP
                     WSATVVDCLVKARADDAFACFDLLPADQKKQFDAESKESFCKYNDCIPHGIEPNGAEI
                     DL"
     gene            complement(16909..>17948)
                     /locus_tag="VMZ53_15170"
     CDS             complement(16909..>17948)
                     /locus_tag="VMZ53_15170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS29844.1"
                     /translation="DPLRADIEQIHRAGVRASEMTRQLLAFSRKQMLQPSAVDVSAVV
                     RGWEKMLGRMIGEDIELRLHAAADAGRAFVDPSQLEQVIMNLVVNARDAMPDGGNLTI
                     ETSSVILDEAAAANAGVTPGSYVLVAVTDTGGGMDRATQSRVFEPFYTTKEQGKGTGL
                     GLSTVYGIVRQSGGNIWLYSELGKGTTFKIYLPRTDRIDEAAAQPASNDSLHGSETIL
                     LVEDEAPVRQVVRSILRKAGYHVLDAENPGEAILVGEQFKGRIHLLLTDVVMPRMSGK
                     DLAERLAPLRPDMSVLFVSGYTENTIVHHGVLDAGVEFLTKPILPDALLRKVRHVLDG
                     AARRAAAQRGD"
BASE COUNT         3072 a         5785 c         5880 g         3211 t
ORIGIN      
        1 gttccgctcg agctcggcgc atcgttgccg ttctcgatcg tcgatggctc ggtcgatggg
       61 caaggcatcg gcgacttcgg cttccatgcg aaggcgcgcg tcgctcgcgt ggggcgcgtc
      121 ggtgttggcg cgatcgcgag cctctacgtg ccgaccggct ccgatcgcaa catgttcgtc
      181 ggtgaagcag caacgacgcc gcagctgatg gcagtcgccg atgcgaacct cggccggctg
      241 cgcctcgcgg tgaacggcgg cgttcgcctc cgtcgcacga cgacgatggg ctccgtgacg
      301 agcgcgacga ccgtgcccgt cggtatcgcc gctgcgtatg cgctctcgcc cgagaaggtc
      361 gagctcgtcg ctgaagtgtt cggctcgctg ccgaccggcg acggttatcg ctcggccgag
      421 gcgctcggcg gtctcaaggt ctacctcgcc aagaattcgt acatgtcgct aggcgccgga
      481 cgtggcctcc tccccggcga agcaggcaac ccagacttcc gcgcagtgat cggcatcgtg
      541 ttcgagccga agcccgcgca gcgcgtctcg cactcgatcg ccgacgacgt cgtggcggaa
      601 atgacgccgc cgaagaaagc gggagacctc gacgccgaca gcgacaacga cctgattcgc
      661 gatcgcgacg atcgctgccc cgacgacatg gaggactacg acggcgaaga ggacgaagat
      721 ggctgcccgg aggcgtcgcg cgagcgcatc gtcgacgtcg gcagcgagct cgttacgctc
      781 cagccgatcg agttcgagtt cgacaaggac gtcctgcgtg acagcgcgtt cccgatcctc
      841 gacgaggtcg tgaaggcgtt catcgacaac tcggacatcg agctggtcga agtccaaggc
      901 cacaccgacg agcaaggcaa cgacgcgtac aacctggacc tctcgaagcg ccgcgctgcg
      961 accgtcatgc gctatctcga cgaccacggc atcgccgcaa gtcgcctgac ctcgaacggc
     1021 tacggcgaga cgaagcccgt cgacaagcgc cacacgcagg acgcgtacaa ggcgaaccgc
     1081 cgcgtgcagt tcgtcatcca gaagcgcggt ggtaatctga tgccttgatt cgcccggctc
     1141 tgttgctcct cgtcttcaca gcttcggcgc gagcccagga gctgaccgac gaagagctgc
     1201 tgaagatgtc ggaagaggag acgatcgagg tctacgacga gcgtcccgac aagccgttcg
     1261 atcgtgacac cgaggtgagg ctgaccggcg aggagctcgc tgcgcgtggc gccgtcgatc
     1321 tcgccaccgc gctcgatctt ctgcccgacg tcgacgttcg tgatggcggc cgcggcggct
     1381 tcaacgtcga tattcgtggt gcacgcaaag gcgcggtcag cgtgctgatc gacggcgtgc
     1441 tcgtgagtga tccgtactac ggcacgttcg acgtctcgac gatcccgatc acggacatcg
     1501 tgcagatccg catgtcgacc tcgccgcaat ctccgatcga cggaccgggc ggcccgggcg
     1561 gcgtgatcga ggtgctgacg cgcgacgcga tcggcccgca gctcgtgctc gtgcgcacga
     1621 ccggcgacac gctaccgagc ttcggtctag cgggctcggc gcgcgctggt ctcgcgaaga
     1681 acctcgcgct tcgcctctcc gcttcgggcc tcgccggcgc gcgcgaattc gatgcgaagg
     1741 gcggtggcct cgacgagaat cgtcatgctg caaccggtgc agcgcgagtc gaataccgcg
     1801 acgatacgcg ccggctcgtc gtcgacgggt ttctcgacga tcgccactac atctcgccgc
     1861 catccgagac cagctcgatc ctcgtgatcg atcgcgagac gaccgagcga gtgtcggcga
     1921 agggcgacgt gcagcgcggc aagctgcagg tgcagggaca agcattcgtc agtcatctgc
     1981 atcgcatctc gcgaaacttc atggacgtga acctgacggt tcaacagcgc gtcgagaact
     2041 tgctcgcact gcgcaccggt gccggcgcgc tcgtcacgaa gccgatcaat caagagatgc
     2101 gctggaccgc gtctgcgagc atcgcgcgcg attcgggtcg cgtggccggc ggcaacaacg
     2161 gtcccgtcga gggccacgtc acgaccctcg aagcggcggg cgacttccag tacgagcgcg
     2221 gtccggcgcg aatcgatgca gcggtgggcg ccgcggtgcc gttcggcatc gacgcaaatc
     2281 cctggcccga ggcgaagctc gtcgcgaaat ggcgcccgtc gtatggcccc gtcgagctca
     2341 ccgcgaccgg tgcgcgcaag ggcagagtgc cgagcctgcg cgagcgattc gatccgtcgc
     2401 agggcggcaa cgatggtctc gatccggaat tcgccacgca cgcagagctg cgcgccgtcg
     2461 ccgagaagaa agacgtctat cgcgtcgagc tcgcaccgtt cttccggcgc acgaacggca
     2521 ccattcgccg ctcgaccgat cccgccgata tgggaatgct cgcgaacctc gatcggctcg
     2581 acgcgaaggg cttcgatctg atcgctagat tcacgccgca tccacgcgtg acggtcggcg
     2641 gcgcgttcga ctacatcacg ctctcgggag gcatgctcga tcgactgccc caacgccgcg
     2701 cggaagcgtg gatccaaggc acgccggaga agcgcatctc tctgctcgcg cgcgtgcgct
     2761 actacggggc gttctacgat caagcgcctt ccggcgcaac cgaaccgaac ctgccggcgt
     2821 acacgaccgt cgaagcgagc gcgacggcga cgctctcgaa gcaatacctc gcggtgctcc
     2881 gcgtcgacga tttactcgat gtccgtcccg agacccatct tggctatttc ggccccggtc
     2941 gcgtcgtgat gctggtgctc caaggccagt gggagtagct gctctaaccg atcgatgcgc
     3001 acagatcgcg gtgtgaaagc cgcgacatcg atgccctgaa atcgcgatga ccgacgcgcc
     3061 atcgtcgccg aaggtcgcga gacgcaacgc acacgatccg gcattcgatt tgctcgtcga
     3121 gaccacatgc gttcgtggag tgggctcgtc gtctgcggct tcttactcgc cgcctgcggt
     3181 ggcgacagtg ggcccggcgg cggtggaagc ttcggcgcga cggccggcgg cgtgaaggac
     3241 atgcgctacg cgcgcgatct gatctcgaag ggcgtcatcc cgccgccgga cgcgatcctc
     3301 gtcgaggcga tgttctccga gcacgacctc gcgctggacg gaccaccatg cgcgcgcacg
     3361 ctctgccttc gctcggccgc cggtttcgcg gactctcgcg gcttcgttca ggtcggcctg
     3421 tcctcgacga tcgatcccga gacgtgggag cggccaccga cgacgttcat cttcacggtc
     3481 gacgtttcgg gctcgatggg ctggggctac ggagacggcg ccacacccgg cgagctatcg
     3541 cgcgctcttc tgcacacgct caccgagcag ctgcgtgaag acgatcaggt cgcgatcgtc
     3601 acgtatggcg atgtcgtgtc gacgaagctc gcgatcacat cgggcgccgc gaagcctgcc
     3661 attcatcaga tcattggagc gctccaaacc gagggtgtca ccgcgatgga gaagggcatg
     3721 cagcgcgcgt acgaggtcgc cgcgaatgcg cccgcctcga cgccgaacgt ccgcgtcatc
     3781 gtcttcaccg atacgcaacc caacgtcggc gcgacgagcc ctgagagctt caacgcgatg
     3841 gtcgcagcag ccgcggacgg cggcgtctac acgacggtgc tcgggctcgg cgtcggtatg
     3901 gcccccgatg tgtttcgcgc gatggcgagc ctgcgcggcg cgaatgcatt cagcttgacg
     3961 tcgcaggtgc acatcgggga gtggttcctc gacgagtggc cgtggttcac gacgccgatc
     4021 gcgttcgact tgcgcgtcaa cgcatcgctg tcgcccggct gggcgatcga gcgcggtttc
     4081 ggctttccgg ccgcgaccga cgcgcaacag gtcggtctca aagcgagcag cgtatttctc
     4141 tcgaagcggc gaggggcgct gctcgtcgag ctggactctc cgaacggcac gccggccggc
     4201 ctcgacggca ccttctcgct cgcatacgtc gacgccaacg gctcgcaggt gacggagaca
     4261 acgcccttcg catatgacgg ctctgctctc gcccgcggcc aatggttcgc gcaacacggc
     4321 gtcgctcgtg cgaccgcgct cgcactgctc accgatgcga tgcaccaagc ggccatcgac
     4381 tacgcggcgc atcctgattt cgcggaggtc acgatgcgtc tcgcatacga ccgcttcacg
     4441 gcagacgcag ctcagctcgg cgacgccgac cttgcgcaag aagtcgactt cgcacggtct
     4501 ctcctcacgc tcatcgaaga gcgcgcagag cagggcgcgc tgtatggcga ttacgccttc
     4561 tgaagctgca cgcgaacctg cagaggacgg tcctgctcgt cgagatcgag gaacaccggg
     4621 aacacgccgt ctccccagct cgtgaagaag aggcatacct tcgcgtcgcc gacttcgatc
     4681 gtgccggcac ctagcttgtt cttgcgcgcc tctgcgagcg cgtagaagtg atgcgagtgc
     4741 gggcggaaat cgatgttcat ggcccacttg ttcgcgtgct tcagcttgtc gacctcgagc
     4801 gccttttcct cggcgacgtc gagcgacagg ttcgtccagc cgtagccctc cttcatcgac
     4861 ttcgcgcccg acgcccttgc agcagccgca gcgtcgcgtc cccagaacac cacgtcggcg
     4921 aggccgtcga gcgtgtcctc gtgcgtccac acgtcgagcg cgctgtgatc catgacgacc
     4981 aagcgcgcga agtccacgct tgcctcgcca agcttcttcc aatgcgtcgg tacacccgac
     5041 gcgagcttga cgatcacgcg gtcccagcaa tcgccgtact ccttgccggc tccgactcga
     5101 tggccgatca ccgcgagcgc gcgatcactc gggacctgcg ccttcacgat ggctggctcg
     5161 agcgcgtcgc ggccgagata gccgagcaga cccacgtcga agacatcgag cgtgccgctc
     5221 ggacacgtga tctcaccgag gagctcgccg ctatgacctg cagacgcgcc gataggccgc
     5281 ttcggagcga ccttcttcgc gactgacttc ttcgcgactg acttcttcgc gctcggcttc
     5341 ttcgcgctcg gcttcttctt ggcgaccggc ttcttcttcg ccgccttcgc cttcggtttt
     5401 gccatccagc gagcttactg cagcgtgccc ttcaccatga cgtagccgtc atccgacgtg
     5461 accgacatgt tcgtcatcac gatgccggcg ggcatggcgg ggagcggaat cgagccgagg
     5521 agcgcgctga tcgagctgat ctgcgagtcg aggcagagct cgacagcctt accgaggtcc
     5581 tcgtccgtca tcatcgtgtg gttctggatt tcgtcctcga cggtgttcgc gtgaatctcc
     5641 ggcttgccga gctcgatcgc gacgccgtag ccgttgagcg ctggcgtgat cttgaggtcg
     5701 accgtcgcgt tgatcgcggc gcggccaacg ggcgagccgt cgagcgtgaa cgtcgccatc
     5761 atgtcgggga ggacgagctt catcttgccg tcgaccgggt cggcgctgat catcgggggc
     5821 gacgtcatcg cgatttccgt cttgttgaac gtgccgccct gcgcgggcat cgagaggttc
     5881 agcatgccgg tcgcaacgag ctggctgacc atcgagttcg cgaggtcgtc ggcgagaccg
     5941 atcatgaggc ccgagcccgg gtccatcgtc gggatgccgt tatccgtgaa gatgaagccc
     6001 ttgctgctct cggtgccctt gatcagcatc ttcatgtcga gcgtgatgag gccgccttct
     6061 gcgtcgaaca tgatgtccga cggcgaaacc tgcatcgaga tcgtcttgcc gaggacgttg
     6121 agctccttcg gaccgccgag gccgccgagc gccttgttca tcatcgggcc catgaacagc
     6181 ttcgccgcga gcggcgccgc cttctcgatg aggctctcga gcggaagcat gtcgaggatc
     6241 gtgccgggga tgcccgacgc cgagatgttg aggccggtga tctgaaccgt ctcgttcttg
     6301 agatccgtct tgaagcccgc catgccgtcc ggcgtgatca tcagcgtgcc cttcacgagg
     6361 acgctcgatg cttgtacgcg gaggttgttc gagccggaga tgcaggccgc ggcgtagcgc
     6421 gcgtggccgg ggacatcgac gccgtcgagc ttgatcgaca tcgagagacc gccgacgacc
     6481 ggcacgagct ggatcgtcgc gttgctcatc ttgaagtcat cgacgtagcc gcgcgcgaac
     6541 aagcagtctt cgccgccttc atcgccggtg tgcgcgatgg gattcatcgg cgtcagcatc
     6601 ggcttgaagt ccatcgcctt gagcatcgtg ccgccggcgc cggcgatctt cgcgaatgct
     6661 tctttgctga tcgcggtggt gaccgcgttt tcgatattgg aaccaggcgc acgaagctga
     6721 ccggcttcga ccgagcgcgt gtcggtggcc ttgccgccgt tcttcgcttc cgccgtcgtc
     6781 gtgatgagcg ttgcgccagc agcgaggttg agctgcgcgg tgaacgtgcc gtccgtattc
     6841 acggtcgcgg cgacgccgtt gaccatgacc ttcttgacgg gagagccatc cacgctcggt
     6901 tgcaccgtac cggtgacctc gatcgaaccc gcgtgatcgc gaatgaggct gcgctgcgga
     6961 gacgtgatct tcaggaccgg cggctcgggc aacgcaccga cttcgtcatt gcaggcggag
     7021 gtgacgagga gcgtcagagt gctcgcgagt aggaggttgc gcatggctgt cttccctttc
     7081 agcaaggggt gcgccactca cgcatgtgcg ctgtcctctc ctcgaacgca acagatcgaa
     7141 ttggttagcc gcggacagat ctcgacgcag tgcgcgaaag aaagctcgat ccgccaccgc
     7201 ggtggcttgc cggtccccat gtccggctag tggactagcc gctgcaacca atcgttagct
     7261 ctggggtatt ggccccactt cgcgcttgta aatttcagct gaccgttacc ttcgcgacgc
     7321 ccttcttcgt caagagatcc ggaaggcgct ttcgaagatc gccttgcagc acgatcgtcg
     7381 tttcctcgac gaagccgccg catccgagtg cttgcttgag ctctttgcac cactgctcga
     7441 gctccgccgc tttcagatcg agtttttcga cgacggtcac ttctttaccg ccacgctgtt
     7501 ttcgttcgta gcgaacgacg gcgcgtttgg gacccggttt tggcgcatcc ggaaccggcg
     7561 caacagtttg ttgcacccct ttgggtaatt ggtcgcgcag gtgctcgagc tgcgagaacg
     7621 gtgaatcgcg tttggtactc atgaggcctt acatcgtcgg ctaaaaacca caatcggaat
     7681 cagcactttg acacttgtga gaagcattcg cacgactcac gatggtgaac atgggcgtaa
     7741 ctgcgaacag ggaagcggtg gtgaaaaccg gtgacatggt cgagggccgt taccggatca
     7801 tcaagacact cggtgcgggc gggatgggca ccgtgtactt ggccgagcac gcgctgatca
     7861 agcgtcgtgt tgcgttgaaa gtcctccacc cggagctcgc tgcggactcc aacgtcgtcg
     7921 agcgcttcat gaatgaagca cgtgcggcag gcacgttggg tcatcccaac atcgtcgagt
     7981 ccacggacat gggcttcacg cacgatcaag ttccgtacat cgtcttcgag tatctcgagg
     8041 gcacgctgct cacggacgag atctatcgcg tcggcggatt gccggtgcgg cgtgctgtcc
     8101 ggatcgcgga ccaaatggct tccgcgctct cggccgcgca caacgcaaac atcatccacc
     8161 gcgatctcaa gagcgacaac atcttcctga ccgacaagga cgacgcgctc gatcacgtga
     8221 aggtgctcga cttcggtgtg tcgcgcttcc tcgaagcgga agagcgtcag cgcggtcttg
     8281 tcgtcggcac gcccgagttc atggcgcccg agcagatcac gagccccgat cacgtggaca
     8341 cgcgtgcgga catctacgcg ctcggcgtga tcatgtacga gatgctgacg gcgcgccgtc
     8401 cgttctccaa tgacgacgac ccgcggcagc tcatgcaccg catcgttcac gagtcgccgc
     8461 cgccgttgca gcgcgcggaa gttccgcatg cgctctcgac gatgatcctc gagcgaatga
     8521 tggcgaagag cccggcgcat cgcttccaga cgatggccga cgtgcagagc gcgctcgagc
     8581 agttcacgac cggtggcctc gcttcgcagc cgcgtcgccg cagcgctccg atcgccgcgc
     8641 agacgatggc gttcgaagat gatcagccgg agacgagcat tcgcctgccg aagccggcgc
     8701 ccgtgcgcaa cacgccgtgg cctgtcgata cgctcgacac gccgatcggc gttggccagg
     8761 tctcgctgcc gatgccaccg cccgtgaagc gtccgtacgc actctacggt gtggcaggcg
     8821 cgggcattct gatgggcatc atcggtctcg tcgtcggcat gcgcggcggt aaggagcaag
     8881 cgccgcagcc agtggcgcag cagcagcagc agatgcagca accgcagcag ctgcccgccg
     8941 ccgcaacgca ggccgcgccg gagaagatcg aagtgaagct gtcagcggac gtgccgaacg
     9001 cgcgcgtggt gttccgccgt cgcgtcgctg atgcgccggt gcagatggag atcaactcga
     9061 cggacgtcgt cgagctcgtc gaagtttcag cgccgggtta caagacgacg cgctactggc
     9121 tgacgttcga tcgcgcgacc tatctcaccg cgcacctcgt caagggcact ggctctgtcg
     9181 aggcgacgga agaagacacg ctcgtcgcgc tcggcgaagt gatcatggtg aaggacaccg
     9241 cgccggcgcc ggcagcagcg cccgtgcagg tcgcgatggc tgagaagcct gtggaagccg
     9301 tcgtggaggc agccaagccg gtcgaggcga aggccgttgt cgctgttgtc gcgccgaccg
     9361 tggcacctcg caagatcggt cgctcggccg cgagcgatga agtcaccgaa gcgacgacgg
     9421 cgccaccggc ggagacgaag gctgaagagc cgaagcccgt ggagacgaag cccctggaga
     9481 cgaagcctgc cgaggcgcag cctgcgatcg cgaagaccga gacggtgctg ccgaaggaag
     9541 agcccgtgca gcagccgaag caagaaccgg tgaagccggt cgagcagccg aaggaagtcg
     9601 tcgccgagcc cgtgcgccct gcgatcgaca aggcgacggt gacgtcggtc atctcgcagc
     9661 atcgccccga ggtgctgaag tgcttctccg agggcaagaa gaagaatgcg cagatgaagg
     9721 gcacgctggg cctgcagctg caggtcgagc cgagtggcaa ggtgttccgc gtgcaggtgc
     9781 agtcgacgct gaacgcgccg ctcgtcgctg cgtgcgtcgt caaggccgcg aatggctgga
     9841 agttcccgac gcggtcgggc ggtgacgtgg cgacggtcga gtacccgttc acgatcaact
     9901 gacgcaaacc caccgctagt gtgtagagtc gggcccgtga gggcccttct cgttttcggc
     9961 gtgtcgttcc tcgtcgcgtg cggcgatgac gcgacgccgc tgcccgaggt caccgactcg
    10021 accgagcacg tcgacccgcg gatcggcacc ggcggccttg gcttcgcgca cggcagctgt
    10081 ttcgtcggtg cgctcgtgcc gcatggcctt gccaagccgg gccccgacac gagcggccca
    10141 ttcggcgtcg tgagctttca gcactactcg ggctacttcg ccgaggacaa caagatccgt
    10201 ggcttctcgc agctgcacct gcatggcacc ggcgcaaccg actacggcgt gctctcgctc
    10261 atgccgacgc tcgcgttcga cacggcaaag cagaaggtca ccgactacga gacgctgttc
    10321 gcgaaagaag acgagcgcgc ggaggctggc tactacggcg tgaagctcgc caatggcatc
    10381 gacgtcgagc tcaccgcgac gacgcgcgtt gccgtccatc gctacacgct gccgtcggcc
    10441 ggcgcggtcg tcgtcgatct cgtgaaggtg ctcgacggcg gcatggtcga cgcgagcgag
    10501 atccatgtcg cgaccgacga ggtgtacggc tcgctgcacc acaagggcgg catgtccggc
    10561 ggcttcggcg gctacacgct gtacttcacg atgcgcggca cggtgccgtt ctcggctgca
    10621 gcggaatgga ccggcggcgc ggcgctctcg ctgcctgcgg gcgcgtcgac catcgcgatc
    10681 ggcttgtcgt tcgtctcgct cgacggcgcg cgcgcgaacc tcgccgccga agtgccgacc
    10741 gtcgacttcg atggcgtgcg tgcaacagcg cgcgatgcat ggcgcgcgaa gctcgacgtc
    10801 gtgaagctca ccggcggcac cgccgagcaa cgccgcacgt tctacacgag cttctatcac
    10861 gcgttcatga tgccgaccgt gatcgacgat cacagcggcg actacgtgct cgcgtattca
    10921 cccgtcgtgc agcaccacga agccggctgg tccatggtct cggacatgtc gctctgggac
    10981 acgtaccgca cgacggtgcc gctctattcg tggctctcgc cgcagagcgc gcgcgatcag
    11041 gtgaagtcgc tcgaaggatt cgcggcgcag ctcggcttct atccgaagtg gccggtcgcg
    11101 atcggcgagt cgggaaccat gcttggctcg agcgcagaga tcgcgatcgc ggacacgatc
    11161 gtccgcggtg gtcagacgga cctcggcgtg aacatgacga tgctgctcac gtcgacgggc
    11221 ggcggtcgcg acaaagtcgc cgactacaca cagtacggct acgtgccgcg cgcgagtggc
    11281 aatcgcacgg cgagcctcac cgtcgagtat gcgcacgatg actacgcgct cgcgcagctc
    11341 accggtgatg cggcgctcct cgagcgaagc cacggttggc gcaagctcta cgacccggcc
    11401 gttggcttcg tgcgtgcgcg caatgcagac ggcagcttcc cggcaggcgc gttcgacgcg
    11461 acgacgtgga tcgatgacta cgccgaagcc gatgcatggc aatcgctgtt cgaagcgggc
    11521 gtccacgatc cggacggcat cgcggagatc ctcggcggta ccgacgccgc ggtcgcgaag
    11581 ctcaccgaat tcttcgagaa ggcgaagaca gactgggaga attccgacga gagcgcgaac
    11641 aacttcccgc gtcgctacta ctgggcgggc aacgagaccg atctcaacgc ggcttacttg
    11701 ttcgcgcagc tcggccgccc cgacttgacg cagaaatggt cgcgctgggc gatcgatacg
    11761 ttcttcacgg atcagccgag cggtgttccg ggcaacgacg acggcggcac gatgggcgcg
    11821 tggtacgttt tcgcgacact cggcctctat cccgtcgccg gcggtgatcg ctacgtgatc
    11881 gctgcgccgc ggtttccgaa agctcgcatc gccatcggcg gccacgagct gaccatcgtc
    11941 gcggacggcg tctccgagag cgccatgtac gtcagctccg tcgatctcga cggcgtgccg
    12001 gtcgacatgc cgtatctcac acaagcgcag ctcaacagcg cgagcacgct gcacttcgtg
    12061 atgaccagta aagcgccgta aaccgcgccg ggctctcgtg gctcttggta tcgtccgttg
    12121 tggagggaac atgagccgac tggtgctgct gtttggattt gctgttgccg cgtgtgttgc
    12181 tcaggcgtcg ccgcaaccga cgagccccgg agggccgccg ccaaatggcg aatacgctgc
    12241 agctccggcc gcgatgccgg cgggtggcca ggtgtgcggc tccggccagg tgtgcaactg
    12301 gacctgcgac ccgggcaact gcaacttcat gtgtcagagc ggcgcgacgt gcaacatgac
    12361 ttgcgatggc ggtaactgcg cgatcgactg ccaaggcggc gcggtgtgca acgcaacgtg
    12421 cgacggcggt aactgtcagg cgcgcggagc caacggagcg tcgctcaatg gcacgtgcga
    12481 cggcggcaac tgcgcgatcg cgtgcgagca cggcgccgtc tgcagcggaa cgtgcgacgg
    12541 cggtaactgt cactagcgct cgcgagcgcg cacgcccacg ctccacgttt tgcatcacta
    12601 gggatcatgc gaatggtccc gagtcttctt ctcgcaacgc tgtgtttcgc gtgcggtaac
    12661 gacgagcgtc cattgccgcc gccgcccgag gtcagaccac cggtgcgtgg cgccgtcggc
    12721 gataccgatc tgcgcgtgat gctcgccgag atcgcctcgt cgaaggcgtg cgagatcatc
    12781 aaggactcgt ttcgggcgct gaagggaacg caggatccga acaccatgac gggcctgctg
    12841 tggatccgcg agtgcaagat cacgaacgcg ggcacgaagg tcacgtacga gcttgccggc
    12901 caaggttggc agtgggccga gcagacgaag aagaaggcag gcggtacgtt cgagctgagc
    12961 gagtacgtga agttcggcgt gaaggcgcac atcgaaggct cgatcgatat cgcgtacgat
    13021 acgaagaatc acatcgcgtc gatctggtac tcgccgaccg agaagccgag cgtcaccttc
    13081 gagccgatcg gtgacgtcga tgtcgaggaa aaaggcgcgt ggtccgaggt cgtcggcggg
    13141 ttgtcgacgc tggtcggctc gtcgccggat cggcagggcg agaagcaggc gaagaagcag
    13201 ggcacgaacg agttcgagag acagctcggc gagggcatga ccgtcgcggt cgatctttgc
    13261 actggctatc agcgcttcac gctcgggcgt cccgacaagg gcgaccttgg cccgccgaac
    13321 gtcggcgagt cattcacgca gccggtcgag ctcgcgaaaa acggcatgat cgtgttcggt
    13381 ccgtacttcg cgcagaacgg catgcacgtg aagctgaaca gcgatggacc agttcgcgcg
    13441 ggcctcgcgt gcgctgacga cgttcacgcg gcggccgatg cattcgtgca cgagcagccg
    13501 ttgccgcaga ttgcgacgct cgcacaggcc gatatcaccg gaactggcac gctgaagatc
    13561 aagccgcagc gctgcaaagt cgtgctcgtc gcgaagtctc tgtcggagaa caaagtgagg
    13621 tttgattggg ctcgcccgcc gcaagaaatc gctcagtcga caggcggtcc agcggttcac
    13681 tgcgcacgca aggggacgct atcctccgcg agcaatgctg ccggatctgg agcctcgcct
    13741 tctaccgccg ctcgtcgacg gtgaggtaga gctccacctc gcggaacgct cgctcgccga
    13801 tccggctcgc aatcggtcgg cgcaataccg gtgggagatt cgcatcggca tctcggtcgt
    13861 cggcaacatc agcttgcggc tcggccatag cgagtacatc gagaagtacg ccggccacat
    13921 cggctatggc atcgacgccg aacatcgcgg tcatcactac gccgcgcgcg cggtgaagct
    13981 cgtgctgccg atcgcgaagt cgcacgattt ccgcgtcgtg tggatcacgt gtaacccgga
    14041 gaacgtcgcg tcgcgccgaa cgtgcgagct cgcgggtggc caactgatcg atatcgtcga
    14101 cttgccgccc gagatcgata tgtacaagga gggcgagcga cagaagtgtc gctatcgcat
    14161 cacgctgtga ccggccgcga cgtcattgcg ttcatcaagc tcggccggcc gatgttcctc
    14221 ggcggcggct tcatcctgta cgcgctcggc gctgtgatcg cggcgagaca cggccacgcg
    14281 atcgactggc gtcactacct cgtcggccaa ggcgcggtca ccgcgttcca gctgatgacg
    14341 cactacgcga acgactactt cgactacgac gcggatcgcc agaacgcgac accgacgaag
    14401 tggtcgggcg gcagccgcgt gttgcccgac ggcgacttgc ctcgccacgt agcgcttgtc
    14461 gccgcgctcg tgttcgctgc gctcggcatc acagcaagca tcgcgcttgc cgcatcgcgt
    14521 gcgggcagcc ttgcggtgcc gacgctcgcg ctcgtgctcg tgctcgcgtg ggagtacagc
    14581 gcgccgccgc ttcgtctgtg cgccgcgggc ctcggcgagc tgacgaccgc gatcgtcgtg
    14641 accgtgctcg tgccgtggct cggcttctat ctgcaagcac ccgcgggtgg cggcggtcgc
    14701 gcgctcgcgt tgacgatcgt gccgctggcg ctgttgcaga tcgccatgct gctcgcgatc
    14761 gagtttcccg acgctgcagg tgatgcagcg accggaaagc ggacgctcgt cgtgcggctc
    14821 ggtgctgcga cggccgcgag attgtatgtc gcgatcaccg cgctcgcgta cttgtggccg
    14881 atcgctgcgc tggcgcttgg tctgccggcg cgcgtcgtcg tcgcactcgc gatcccagcg
    14941 cccatcgcga tctggcggat gatgcgaatc gccgaccatg ccgatgcctc cgccttcgag
    15001 cgcctgacgt tctgggctgt gttcttgctc gtcgcgacgt ctgccctggc gctcggcgcg
    15061 tggctcactc tatgatgagg tgatgaccta cgacgcgatc atcgtggggg cgcgctgtgc
    15121 aggggctccg ctcgcacggt tcttggcacg tgctgggaag aaggtcgcgc tcgtcgattc
    15181 ggcgacgttt ccgagcgacc agccgatgtc gacgcacttc attcatccgt acgggatgcg
    15241 cgttctcgag gatctcggcc tggcggagcg gatccgagat atctcgccgc cggtaaatct
    15301 gatgaggcag gacgtcggcg gccacatcgt cgagtttcga atcaacccgg gcggttcgtg
    15361 cccgcgtcgc atcgatctcg atcaagttct tctcgacgga gcgcgcgaag caggtgcgga
    15421 ggttcggctc ggcgagcgtg tcatcgagat tatccgtgag aagtcgcgcg tcgtcggtgt
    15481 ggtaaccgca tcacgcgagg cgacgcgtca cgagctgcgc ggcaatgtcg tggtcggtgc
    15541 agacggcagg cactcgacga tcggcgatct agtagaagcc gaacgctact tcgagtacgt
    15601 ggggccacga ggtgcgtact gggcgtactg gccgcgtccg aagttcttcg acgaagcccc
    15661 atacaatggt gcgacgatgc tgcgcttcgt cggcgagcat tttcagttcg tctttccaac
    15721 gaatcgcgat caactgatct tgggcgtcgg cttcccgcgc gaggagctcg cgacgtggaa
    15781 gacggacccg cacgcgaagc ttctcgagca tctccgctcc acccctttct tcaagccgct
    15841 cgtcgaggcg caaccgatct cgaagacgct aggcctcacg aaagcggagt acttcttccg
    15901 gcgcgcggca ggacccggct gggcgctcgt cggggacgcg ggcctgttca aggatccgac
    15961 gcccggcctc ggcatcaccg atgcactgcg cgacgcgaag aacctggcgg ccgcgatcat
    16021 cgaaggcggc gaccaggccc tcgagcgtta ttggcggcaa cgcgacgtcg actccatcga
    16081 gctgttcaac tttgcgcgcg atatgggcga gctcttctac aacaacgcgc taactcgcat
    16141 gctcttcgcg aagctcgaga ccgacccgga gctcggtcag cgaatggtgc gcgtgatcga
    16201 gcgcgagatt tcaccgtacg gcgcgttcaa gccgagcgag gtcgttcgct ggacgttcgg
    16261 agcgctgctc cgcggaaagc tcggcgtact gcctgcgttc tttcgcgcag ggtttcgcgg
    16321 agcgcaggtg cagaaggagc tgaagcgtcg ccgcgcgttg ctcgtcccgg cgttgccggc
    16381 atcgccggtg ctcgccgaga aggccgccta gagatcgatc tctgcgccgt tcggctcgat
    16441 gccgtggggg atgcagtcgt tgtatttgca gaagctctcc ttcgactcgg cgtcgaattg
    16501 cttcttctgg tccgccggca acaagtcgaa gcacgcgaac gcgtcgtcgg cgcgagcctt
    16561 caccaggcag tcgacgacgg ttgcggacca cggcgtctcg acgcagcgct tgcggatgat
    16621 ctcgtcgtac tcgcgtcgtt cttcgttgct gtacgtgcgg ccgaggtggc gttttgcgat
    16681 cttcgtgcag tcgcgcgttt gcagtgcggc gggcttcggc ggtgcgagca ccatcgcgtc
    16741 gacagccggc gcgtcatcga gatgcgcgtc gacaggcgat gcatcgaggc gtgccgcggt
    16801 tgcgtcacgt ggcgcaggcg caggcggtgg ttgcttcgcc gatcctcggt cacacgcgac
    16861 aagcatcacc gcgacagcca ctcggagttt ccgcatatgg atgaagagtc agtctccgcg
    16921 ctgcgcggcg gcgcgccggg cggcgccatc gaggacgtgg cgcactttcc gcaagagggc
    16981 atccggcaga atcggcttgg tgagaaactc gacgcctgcg tcgagcaccc cgtggtgcac
    17041 gatcgtgttc tccgtatagc cggagacgaa gagaacgctc atgtcaggac gtaacggcgc
    17101 gagccgctcg gcaagatctt ttccgctcat gcgcggcatg acgacgtcgg tgaggagcaa
    17161 atggattctg cctttgaatt gctcgccgac gagaatggct tctccgggat tctcagcgtc
    17221 gaggacgtgg tagccagcct tgcgtagaat gctgcgcacg acttgtcgta cgggcgcctc
    17281 gtcttcgacg agcaggatgg tctccgatcc gtgcagcgag tcgttggacg ccggctgcgc
    17341 cgctgcttcg tcgatacggt ccgtgcgagg caggtagatc ttgaaggtcg ttcctttgcc
    17401 gagctcgctg tagagccaga tgttcccgcc actctgtcgc acgattccgt agaccgtcga
    17461 gagcccgagg cccgtgcctt tgccttgctc cttggtcgtg tagaacggct cgaacacgcg
    17521 gctctgcgtc gctcgatcca tcccgccgcc ggtgtcggtg acagccacga gaacatacga
    17581 gcccggcgtc acgccggcat tcgccgcagc cgcctcgtcg agaatcacgc tcgacgtctc
    17641 gatcgtcaga ttcccgccat ccggcatcgc gtcacgcgcg ttgacgacga ggttcatgat
    17701 cacctgttcg agctgactcg ggtccacgaa cgccctcccc gcatctgccg cggcgtgaag
    17761 acggagctcg atatcctcgc cgatcatgcg tccgagcatc ttttcccagc ctcggacgac
    17821 ggcgctgacg tcgacggcgc tgggctggag catctgcttg cgactgaaag cgaggagctg
    17881 tcgcgtcatc tcgctcgcgc gcactcctgc gcgatggatt tgctcgatat cggcgcgcag
    17941 aggatcgg
//
LOCUS       DATLBZ010000292        10137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4039123, whole genome shotgun sequence.
ACCESSION   DATLBZ010000292 DATLBZ010000000
VERSION     DATLBZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4039123"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /gene="ureC"
                     /locus_tag="VMZ53_15175"
     CDS             <1..1665
                     /gene="ureC"
                     /locus_tag="VMZ53_15175"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HUS29845.1"
                     /translation="VGDRVRLGDTDLWLEVERDFAHPGDELTFGGGKVLRDRMGQTEG
                     VSDIEALDLVITNALIVDWTGVYKADVGVKGGRIVGIGKAGNPRVMAGVTPGMMVGVT
                     TEAIAGEGMILTAGGIDTHIHFICPQLADEAIASGITTFVGGGTGPTTGTNATTCTPG
                     ARHIELMLQATDNIPLNIGLTGKGNSSLSNGLVDQIRAGAIGLKLHEDWGTTPAAIDC
                     CLSVADDEDIQVTIHTDTLNESGYVDDSIAAFKGRTIHTYHTEGAGGGHAPDIMRVCG
                     EPNVIPSSTNPTRPYTVNTLDEHLDMLMVCHHLDREIPEDVAFAESRIRGETIAAEDI
                     LHDIGALSIISSDSQAMGRVGEVITRTWQTAHKMREQRGKLPSERGDNDNNRIRRYVA
                     KYTINPAIAHGLSHDIGSIELGKLADLVLWRPAFFGIRPELVLKGGFIAWAQMGDANA
                     SIPTPEPMLMRPMFGARGKAIGRTSLAFISERALQEGHVVNLGLTKQLSVVRGCRGIG
                     KKHMKLNDALPKLEIDPETYEVRADGVALTCEPAKTLPLTQLYSLF"
     gene            1665..2291
                     /locus_tag="VMZ53_15180"
     CDS             1665..2291
                     /locus_tag="VMZ53_15180"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HUS29846.1"
                     /translation="MTSWIVLQLADSAFPTGGFAHSAGLEAHVQAGELASVEVYAREY
                     LDQLALGSLPLVAAVHDDRARIAEVDALARATLWNHVAARASRMQGRALLDVAARSFG
                     VAKPTGIEAHLAPAFGYVTRSLGVDRDEALATFLHLALRGLLSAAVRLGCAGPNEAQQ
                     LHHALHSALDAALARGRALTLADVGQPSPLAELIAGTHDRLYSRLFQS"
     gene            2337..3008
                     /gene="ureG"
                     /locus_tag="VMZ53_15185"
     CDS             2337..3008
                     /gene="ureG"
                     /locus_tag="VMZ53_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HUS29847.1"
                     /translation="MDHPGHFHERDAMRRRDFTQRAFTVGIGGPVGSGKTALVLSLCR
                     ALRNELRLGVVTNDIFTREDAEFLTRNEALPTAQIRAVETGGCPHAAIREDISPNLMA
                     LEDLMTAVRPELLVVESGGDNLAAQYSRELVDYTIYVIDVAGGDKVPRKGGPGITQSD
                     LLVINKTDLAPHVGADLGVMERDAKKMRGSGPTVFAQVTKDIGMSDVIRHILHARAHA
                     LGVEH"
     gene            3024..6773
                     /locus_tag="VMZ53_15190"
     CDS             3024..6773
                     /locus_tag="VMZ53_15190"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUS29848.1"
                     /translation="MDEQQLFRQALVHSPEGVSVYELDGTLVWMNPATERMMGVSLAE
                     LQGKRIFELYPDGLGTPFHAAFLRVVEGAPAQQFEQYYPRFDGWYSLCVVRVGDRVHA
                     YARETTTDIRHRRRLETLARISEVVTADEQATTSTARAVSQIITDVIDADCTIALLSD
                     DSQWLEVSARTSKDSTTLELMNTVMRWPSDTGTPGDALRTRQVVLVAANPKDVTTDHT
                     PELRQLVARYQPSAICVGPLVVGDAAIGVIIVTRREGRPPLTEDDRALMSAIGPSVAL
                     YIALARRRAESAIAQQRLGLVADSLPALVAFIDRDERYQYVNATYAAWLGARASDVIG
                     KRPRDMFRNEYPAIEEFVRRALAGETVRFRTRFAYPTGVREVDAQYVPVHAPSGEQTG
                     FAVLVQDISGEARIAELERERLEAERNLNARLQAFLSVTGQLAGATTTDDIARILVDE
                     GVRALDASIAGLWLRSGDDAALTLVRHANFRPEFQRSFARVPIAASEPLGDCVLSGKP
                     VFISSRTEYVARYPSFESTHRPDPSPPISFALLPLAFEGRVAGCINFSFHDERRLTAD
                     ERTYLELLASHGAEALRRAKVASELREVSETREAMIHASPAAIAVTDGDAIVRVWNPA
                     AETILGVSAADAIGKPHMFAADPAWRRDLDAVLAGNTIHGRQVRRVRRDGTLFDVELY
                     AAPVALGDGRVVALSMVVDVTERKRVERGRQMIANATTIFNRSLDLEETLRQIVRVPL
                     GGWAEWCGVDILGDDGMLDRISLSLEDSGPRLPARLAHDPRYSGSSSAIARRETFILR
                     DATDEDLQTIARNADHLRALRMLRLRSVLSVPLVAGERVLGAITIGTSNHNFDDLDAS
                     ILAELATLATTAIENARLYKDAQLARGEAEAASRAKDEFMAMLGHELRNPLAPIVTAL
                     DLMQLRAPGQFERERAVIGRQVHHLSRLVDDLLDVSRITRGKVELHKERLAVAAIVAK
                     AIEQASPLLEQKNHRLVIDVPYDLHVYGDPLRLAQIVSNLVGNAAKYTPDAGLVEVSA
                     RGEGDRVVLTVRDNGIGITPEMLPHVFDMFVQAPQSSSRPAGGLGLGLTIVKSLVDMH
                     GGTVRVESAGKNKGSSFSVSLPIATERIAVVESAALMQIACTAVPRRILVVDDNEDAA
                     MLLASVLEAHGHDVRTAFDGPSALRIANEFQPEIAVLDIGLPVMDGYELAERLLTEFA
                     GVRLIAVSGYGQESDRARTRAAGFQAHLAKPVPIDALVRLVDESS"
     gene            6841..7338
                     /locus_tag="VMZ53_15195"
     CDS             6841..7338
                     /locus_tag="VMZ53_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29849.1"
                     /translation="MRLAIGVVLILGACGGDDGGAQKDAAVDSKAIDAPIDGKTFLDA
                     PAGTTALTIKNELAWCEISVMGGTASTASTITTNVTPGSITLVAKAAPGAGSAPSAFK
                     IAPNMWHHTTNDTGTGETGTVAGTGDAATSTVMALVVSGTPKCVWVCCPFKNGTGCGT
                     TDLCP"
     gene            complement(7335..8009)
                     /locus_tag="VMZ53_15200"
     CDS             complement(7335..8009)
                     /locus_tag="VMZ53_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29850.1"
                     /translation="MTRIAVTVLVLVAAARTAHAGWDCPEKHSDAIKNIETYAKNPKA
                     FEPPSDTSFGPDFLCADGVAKEFPDRVAAACKTILDRKGDKHIDDCIDLAAFAGLTQV
                     GDHDIFALIAARKENPLSYPGGFWWPRLNFLGAMRDARGLPIIIDEWTDASAIAPKKK
                     GWGAVKSWALWRRTACNMLASFGGADEVAFLEREVAATRGKDARSVKNACNAAITAIK
                     KRLGTP"
     gene            8065..9687
                     /locus_tag="VMZ53_15205"
     CDS             8065..9687
                     /locus_tag="VMZ53_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HUS29851.1"
                     /translation="MAPVKKPETPQEFKTMSGIPLKAAYTPQDLESRGWTYAEKLGDA
                     GSFPYTRGPHATMYRGKPWTMRMFAGFGTPEDTNKRFKFLLDSGQTGLSTAFDMPTLM
                     GYDPDHARSLGEVGREGVSVATLDDMFRLFGDIPLDKVSTSMTINAPAAVLLALYVAV
                     AEKQGVSPDKLRGTLQNDMLKEFIAQKEWISPIRGHMRIIRDMLVWCTKTMPQWNTIS
                     ISGYHIREAGATAVQELAFTIADGIGYVQLGIDAGLDVDDFAPRLSYFWDVHNDFFEE
                     IAKFRAGRRMWAKIMKERFGAKNPKSMLLRTHAQTAGVSLMAQQPLNNVVRTTMQALA
                     AVLGGTQSLHTNSYDETYALPTEDAAQLALRTQQVIYEETNVANVVDPLAGSYFIETL
                     TDQVEAAAWEYIHKIDAMGGITKAVEEGYPQREIARSAFEFQREVDSGRRSVVGVNKY
                     QSPDEGDHIPTLKIEQEIEHNQIAQVKKFRSARDAAKVEAALAAVKAALADDKQNVMQ
                     PIFEAVKANATLGEICDLFRTELGEYRDPAYL"
     gene            complement(9741..>10137)
                     /locus_tag="VMZ53_15210"
     CDS             complement(9741..>10137)
                     /locus_tag="VMZ53_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29852.1"
                     /translation="SDCIVWSLEVGAKTIGKLDLFVQFAKLFPIDAFEGHSFGMYAFM
                     DDARALPAIRDAWLRAAADKRSKNPGRNQKYLLAVWHHDALRLFTSKGTAEDAKFLAE
                     QLPKASDKGVRRGMHKTIDAIKKRDPAAK"
BASE COUNT         1762 a         3377 c         3327 g         1671 t
ORIGIN      
        1 gtcggcgacc gcgtgcgcct cggcgacacc gatctctggc tcgaggtcga gcgcgacttc
       61 gcccatcccg gcgacgagct gacgttcggc ggcggcaagg tcctgcgcga tcgcatgggc
      121 cagaccgagg gcgtctccga catcgaggcg ctcgacctcg tgatcacgaa cgccctgatc
      181 gtcgactgga ccggcgtcta caaggccgac gtcggcgtca agggcggccg catcgtcggc
      241 atcgggaagg ccggcaaccc gcgcgtcatg gccggcgtca cgcccgggat gatggtcggc
      301 gtcaccaccg aggcgatcgc cggcgaagga atgatcctga ccgcgggcgg catcgatacg
      361 cacatccatt tcatctgccc gcagctcgcc gatgaagcga tcgcgagcgg catcacgacg
      421 ttcgtcggcg gcgggactgg gcccaccacg gggacgaacg cgaccacgtg cacaccgggc
      481 gcgcgccaca tcgagctgat gttgcaggcg accgacaaca tcccgctgaa tatcgggctg
      541 accggcaagg gcaacagctc gctctcgaac gggctcgtcg atcagatccg cgcgggcgcg
      601 attggcctca agctgcacga ggattggggc acgacgcccg cggcgatcga ctgttgtctc
      661 tccgtcgccg acgacgagga catccaggtc acgatccaca ccgatacgct caacgaatcg
      721 ggctacgtcg acgattcgat cgctgccttc aaggggcgaa cgattcacac gtatcacacc
      781 gagggtgcag gcggcgggca cgcaccggac atcatgcgcg tgtgcggcga gcccaacgtg
      841 attccgtcat cgacgaatcc aacgcggccc tacaccgtca acacgctcga cgagcacctc
      901 gacatgctca tggtgtgtca ccacctcgat cgcgagattc ccgaggacgt cgcgttcgcg
      961 gagagccgca ttcgcggcga gacgatcgcg gccgaggaca ttctccacga catcggcgcg
     1021 ctctcgatca tctcgagcga tagccaagcg atggggcgcg tcggcgaggt catcacgcgc
     1081 acgtggcaga ccgcgcacaa gatgcgcgag cagcgcggca agctgccgag cgagcgcggc
     1141 gacaacgaca acaatcgcat tcgccgctac gtcgcgaagt acacgatcaa tccggcaatc
     1201 gcgcatggcc tgtcgcacga catcggcagc atcgagctcg gcaagctcgc agacctcgtg
     1261 ctgtggaggc cggcgttctt cggcattcgg ccggagctcg tgctgaaggg tggcttcatc
     1321 gcgtgggcgc agatgggcga tgcgaatgcg tcgatcccga cgcccgagcc gatgctcatg
     1381 cgaccgatgt tcggtgcgcg cggcaaggcg atcggccgca cgtcgctggc gttcatctcg
     1441 gagcgcgcgt tgcaagaagg acatgtcgtc aacctcgggc tcaccaagca gctgtcggtc
     1501 gtgcgcggct gccgcggcat cggcaagaag cacatgaagc tcaacgacgc actgccgaag
     1561 ctcgagatcg atcccgaaac gtacgaagtc cgcgccgacg gtgtggcgct cacgtgcgag
     1621 cccgcgaaga cgctgcctct cacccagctg tactcgctgt tctgatgacg agctggatcg
     1681 tcctgcagct cgcggactcg gcgttcccga cgggaggctt cgcgcattcc gcgggtctcg
     1741 aggcgcacgt gcaggcgggc gagctggcga gcgtcgaggt gtatgcacgc gagtatctcg
     1801 atcagctcgc gctcggctcg cttccgctcg tcgctgcggt tcatgacgat cgtgctcgca
     1861 tcgcagaagt cgacgcgttg gcgcgcgcca cgctgtggaa tcacgtcgct gcgcgtgcca
     1921 gccgcatgca gggccgcgcg cttctcgatg tcgcggcccg cagcttcggc gtcgcgaagc
     1981 cgaccggcat cgaagcgcat ctcgcacccg cgttcggcta cgtcacgcgc tcactcggcg
     2041 tggatcgcga cgaggcgctc gcgacgttct tgcatctcgc gctgcgcggt ctgctgtccg
     2101 ccgccgtgcg cctcggctgt gcgggaccga acgaagcgca gcagcttcat catgctctgc
     2161 actctgcgct cgacgccgca ctcgctcgcg gccgcgcatt gacgctcgcc gatgtcgggc
     2221 agccctcccc tctcgccgag ctgattgccg gcacgcatga tcgtctctac tcgcgcctct
     2281 ttcagagctg aggacctatg ggccacgaca tcgacacccc acatacgcac gagcacatgg
     2341 accaccccgg ccactttcac gagcgcgatg cgatgcgccg tcgcgacttc acgcagcgcg
     2401 cgttcaccgt cggcatcggc ggtccggtcg gcagcgggaa gacagcgctc gtgctctcgc
     2461 tgtgccgcgc actccgcaac gagctgcgcc tcggcgtcgt cacgaacgac atcttcacgc
     2521 gcgaggatgc cgagtttttg acgcgcaacg aagcgctgcc gaccgcgcag atccgtgccg
     2581 tcgagacggg cggctgccca cacgcggcga ttcgcgagga catctcgcca aacttgatgg
     2641 ccctcgagga tctgatgacg gccgtgcgcc ccgagctgct cgtcgtcgag agcggtggcg
     2701 acaacttggc cgcgcagtac agccgcgagc tcgtcgacta tacgatctac gtgatcgacg
     2761 tcgcgggcgg cgacaaggtc ccgcgcaagg gcggcccggg gatcacacaa agcgacttgc
     2821 tcgtgatcaa taagacggac ctcgcgccgc acgtcggagc tgatctcggc gtcatggagc
     2881 gcgacgcgaa gaagatgcgc gggagtggtc cgacggtctt cgcgcaggtg accaaggaca
     2941 ttggaatgtc cgacgttatt cggcacatcc tgcatgctcg cgcgcacgcg cttggcgtcg
     3001 aacactgata ggttcgcccc gcagtggacg agcagcagtt atttcgacag gcgctcgttc
     3061 actcgcccga gggtgtctcg gtctacgagc tcgacggcac gctcgtctgg atgaatccag
     3121 ccacggagcg aatgatgggc gtgtcgctcg cagagctcca ggggaagcgc atcttcgagc
     3181 tgtaccccga tggcctcggc acgccgtttc acgcggcgtt cctgcgcgtg gtcgagggcg
     3241 cgcccgcgca acaattcgag caatactacc cgcggttcga cggctggtat tcgctgtgcg
     3301 tcgtccgcgt gggcgatcgt gtccacgcgt acgcgcgcga gaccaccacc gatattcgtc
     3361 atcgccgccg gctcgagacg ctcgcgcgga tctcggaggt cgtcactgcg gacgagcaag
     3421 cgacgacgag cacagcgcgc gcggtctcgc agatcatcac agacgtcatc gacgccgact
     3481 gcacgatcgc cctcttgtcc gacgactcgc agtggctcga ggtctccgct cgcacgtcga
     3541 aagactccac gacactcgag ctgatgaaca cggtgatgcg gtggccgtcg gacaccggca
     3601 cgccaggcga tgcgctgcgc acgcggcagg tcgtgctcgt cgcggcgaat ccgaaggacg
     3661 tcacgacgga ccacacgccg gagctgcgcc agctcgtcgc gcgctatcag ccgtccgcga
     3721 tctgcgtcgg tccactcgtc gtcggcgatg cagcgatcgg cgtcatcatc gtcacgcggc
     3781 gtgaggggcg ccctccgctc accgaggatg accgcgcgct gatgtccgcg atcgggccaa
     3841 gcgtggcgct gtacatcgcg ctcgcacgtc gccgtgccga gagtgcaatt gcccagcagc
     3901 gcctcgggct cgtggcggat tcacttccgg cgctcgtcgc attcatcgat cgggacgagc
     3961 ggtatcaata cgtcaacgcg acgtacgcgg cgtggctcgg cgcgcgagcg agcgatgtca
     4021 tcggcaagcg cccgcgtgac atgttccgca atgagtatcc ggcgatcgaa gagttcgtgc
     4081 ggcgcgcgct cgcaggagaa accgttcgct tccgcacgcg attcgcctac ccgaccggcg
     4141 tgcgagaggt cgacgctcag tacgtcccgg tgcacgcgcc ctctggcgag caaacgggct
     4201 tcgcggtgct ggttcaggac atcagcggcg aagcgcgcat cgccgagctc gaacgcgaac
     4261 gcctcgaggc cgaacgcaac ctaaatgccc ggctgcaggc attcttgtcc gtcaccggcc
     4321 agcttgcggg tgcgacgaca acggacgaca tcgctcggat cctcgtcgac gaaggcgtgc
     4381 gcgcgctcga tgcttcgatc gcgggattgt ggctgcgctc cggcgatgac gcggctctga
     4441 cgctcgtccg gcacgcgaac ttccgccccg aattccagcg ctccttcgca cgcgttccga
     4501 tcgcagcaag tgagccactc ggcgactgcg ttctgtccgg aaagccggtg ttcatatcga
     4561 gccgcacgga gtacgtcgcg cggtatccga gcttcgagag cacgcatcgg ccggatccgt
     4621 cgccgccgat ctcgttcgct ttattgccac tcgcattcga aggtcgcgtc gcgggctgca
     4681 tcaacttctc gtttcacgac gagcgccggc tcaccgccga tgaacgcacg tatctcgagc
     4741 tgctcgcgtc gcacggggct gaggcgctgc gccgcgcgaa ggtcgcgagc gagctgcgcg
     4801 aagtcagcga gacccgcgag gcaatgatcc acgcgtcgcc agccgcgatc gcggtgaccg
     4861 acggcgacgc catcgttcgg gtctggaatc cggcggctga aacgatcctc ggcgtctctg
     4921 ccgccgacgc gatcggcaag ccccatatgt tcgcggcgga tcccgcatgg cggcgcgacc
     4981 tcgacgctgt cctcgccggc aacacgatcc acggacgtca ggttcggcgc gttcggcgtg
     5041 acggcaccct cttcgacgtc gagctctatg ccgcaccggt cgcgctcggc gatggccgcg
     5101 ttgttgcgct ctcgatggtc gtcgacgtga cggagcgcaa gcgcgtcgag cgcggccgtc
     5161 aaatgatcgc gaacgcgacg acgatcttca atcgctcgct cgatctcgag gagacgcttc
     5221 gccagatcgt gcgcgtgccc ctcggtggtt gggccgagtg gtgcggtgtc gacatcctgg
     5281 gcgacgacgg catgctcgat cgtatctcgc tttccctcga ggattccggc ccccgcctac
     5341 ccgcgcgtct cgctcacgat cctcgctaca gtggctcgag cagcgcgatc gcgcgccgtg
     5401 aaacgttcat cttgcgcgac gcgaccgacg aagatctgca gaccatcgcg cgcaacgccg
     5461 accacctgcg cgccctgcgg atgcttcgcc tgcgctcggt gctctcggtc ccattggtgg
     5521 caggtgaacg cgtgctcggt gccatcacga tcggcacgag caaccacaac ttcgacgacc
     5581 tcgatgcgtc gatcctcgcc gagctcgcga cgctggcgac aacagcgatc gagaacgcgc
     5641 gcctctacaa agacgcccag ctcgcccgcg gcgaggcaga ggcggcgagc cgagcgaagg
     5701 acgaattcat ggcgatgctc ggtcacgagc tgcgcaaccc gctcgcaccg atcgtcacag
     5761 cgctcgatct catgcagctc cgcgcgcctg gccaattcga gcgcgagcgc gccgtcatcg
     5821 gccgccaggt ccaccacctc tcacggctcg tcgacgatct gctcgatgtc tcgcgcatca
     5881 cgcgcggcaa ggtcgagctg cacaaagagc ggctcgccgt cgctgccatc gtcgcaaagg
     5941 cgatcgagca agccagcccg ctgctcgagc aaaagaacca tcgcctcgtg atcgacgtgc
     6001 cgtacgacct gcacgtctac ggcgacccgc tgcggctcgc gcagatcgtc agcaacctcg
     6061 tcggcaacgc cgccaagtac acgcccgacg cgggcctcgt cgaagtgagc gcgcgcggcg
     6121 aaggcgaccg cgtcgtgctg accgttcgcg acaacggcat cggtatcacc cccgagatgc
     6181 tgccgcatgt gttcgacatg ttcgtgcagg cgccgcaatc gagctcgcgt cccgcaggtg
     6241 gtctcggcct cggcctcacg atcgtaaagt cgctcgtcga catgcacggc ggcaccgtgc
     6301 gcgtagagag cgctggcaag aacaagggct cttcgttctc ggtgtcgctg ccaatcgcga
     6361 ccgaacgcat cgccgtcgtc gagtccgcgg cgctcatgca gatcgcgtgt acggcggtac
     6421 cgcgccgcat cctcgtcgtc gacgacaacg aagatgccgc gatgctcctc gcgtcggtgc
     6481 tcgaagcgca cggccacgac gtccgcacgg cgttcgatgg tccctccgcg ctgcgcatcg
     6541 ccaacgagtt ccagcccgag atcgcggtgc tcgacatcgg cctgccggtg atggacggct
     6601 acgagctggc cgagcgcctg ctcaccgaat tcgcgggcgt gcggctgatc gccgtaagtg
     6661 gctacggcca agaatccgac cgcgcgcgca cgcgtgccgc gggcttccaa gcgcacctcg
     6721 ccaagccggt gccgatcgac gcgctcgttc gcctcgtcga cgagagcagc tgagcgcgga
     6781 acgcattgtt gcggcggcga tcacagcatg ccgccatcgt ctcgtgccta tagtgcgctc
     6841 atgaggctag cgattggtgt ggtgttgatc ctcggtgcgt gcggtggcga cgacggcggc
     6901 gcgcaaaagg atgcggccgt cgactcgaaa gcgatcgatg cgccgatcga cggcaagacg
     6961 ttcctcgacg cgccagcggg tacgacggcg ttgacgatca agaacgagct tgcctggtgt
     7021 gagatcagcg tcatgggtgg cacagcgtcg accgcgtcga cgatcacgac gaacgtgacg
     7081 cccggaagca tcacgctcgt tgcgaaggcg gcacctggtg caggcagcgc tccgagtgcg
     7141 ttcaaaatcg cgccgaacat gtggcaccac acgacgaatg acacggggac gggtgagact
     7201 gggacggtcg ctggtacggg tgacgctgcg accagcacag tgatggcgct cgttgtttca
     7261 ggaacgccga agtgcgtctg ggtctgctgc ccgttcaaga acggcacggg ctgcggcaca
     7321 acggatttgt gtccctaggg cgttcctagc cgcttcttga tcgcggtgat ggccgcattg
     7381 cacgcgttct tcacggagcg cgcgtccttc ccccgcgtcg cggccacctc gcgctccagg
     7441 aaggcgacct cgtcggcacc gccgaagctc gcgagcatgt tgcacgcggt tcggcgccag
     7501 agcgcccagc tctttaccgc gccccagcct ttcttcttgg gagcgatcgc ggacgcgtcg
     7561 gtccactcgt cgatgatgat cgggagccca cgcgcatcgc gcatcgcgcc gaggaagttg
     7621 agccgcggcc accagaagcc gcccgggtac gacagcgggt tctctttgcg tgcagcgatc
     7681 aacgcaaaga tgtcgtggtc gccgacttgg gtgaggccag caaaggccgc gaggtcgatg
     7741 cagtcgtcga tgtgcttgtc gcccttgcgg tcgaggatcg tcttgcacgc ggcggcgaca
     7801 cggtccggga attccttcgc gacgccgtcg gcgcagagga agtcggggcc gaagctcgta
     7861 tcgctcggcg gctcgaatgc tttggggttc ttcgcgtagg tctcgatgtt cttgatcgcg
     7921 tcgctgtgct tctcggggca atcccagccg gcgtgggcag ttcgggccgc agcgacgagg
     7981 acgaggaccg ttacggcgat gcgcgtcatg ggtccggaga aaacgccccg atcggcgcga
     8041 cgattcccgt ggtaagtgaa ggccatggcg ccggttaaga agcccgagac cccgcaggaa
     8101 ttcaagacca tgagcggcat cccgctcaag gcggcgtaca cgccgcagga cctcgagtcg
     8161 cgcggatgga cctacgcgga gaagctcggc gatgcgggaa gctttccgta cacgcgcggg
     8221 ccacacgcga cgatgtatcg cggcaagccg tggacgatgc gcatgttcgc gggcttcggt
     8281 acgccggaag acacgaacaa gcgcttcaag ttcttgctcg acagcgggca gaccggtctg
     8341 tcgaccgcct tcgatatgcc gacgctcatg ggctacgacc cggaccatgc gcgctcgctt
     8401 ggcgaagtcg gtcgcgaggg cgtgagtgtc gcgacgctcg acgacatgtt ccgcctgttc
     8461 ggcgatatcc cgctcgataa ggtgtcgacg tcgatgacga tcaacgcgcc ggcggctgtg
     8521 ctgctcgcgc tctacgtcgc ggtggccgag aagcagggcg tctcgcccga caagctgcgc
     8581 ggcacgctac agaacgacat gctcaaggag ttcatcgcgc agaaggagtg gatcagcccg
     8641 atccgcggcc acatgcggat catccgcgac atgctcgtgt ggtgcacgaa gacgatgccg
     8701 cagtggaaca cgatctcgat cagcggctat cacatccgcg aagctggcgc gacggccgtg
     8761 caggagctcg cattcacgat cgccgacggc atcggctacg tgcagctcgg gatcgatgcg
     8821 ggcctcgacg tcgatgattt cgcgccgcgc ctctcgtact tctgggacgt ccacaatgac
     8881 ttcttcgagg agatcgcgaa gttccgcgcg ggccgccgga tgtgggcgaa gatcatgaag
     8941 gagcgcttcg gcgcgaagaa cccgaagtcg atgctgcttc gcacgcacgc gcaaacggcc
     9001 ggcgtgtcgc tcatggcgca gcagccgctc aacaacgtcg tgcgcacgac aatgcaagcg
     9061 ctcgccgcgg tcctgggcgg cacgcagtcg ctgcacacga acagctacga cgagacctac
     9121 gcgctgccga cggaagacgc cgcgcagctc gcgctccgca cgcagcaagt catctatgaa
     9181 gagacgaacg tcgcgaacgt cgtcgatccg ctcgcgggca gttacttcat cgagacgctg
     9241 acggaccaag tcgaagccgc cgcgtgggag tacatccaca agatcgacgc gatgggcggc
     9301 atcacgaagg ccgtcgaaga aggctacccg cagcgcgaga tcgcgcgctc tgcattcgag
     9361 ttccagcgcg aggtcgacag cggccgccgc tccgtcgtcg gcgtgaacaa gtaccagtcg
     9421 cccgacgaag gcgatcacat ccccacgctc aagatcgagc aagagatcga gcacaaccag
     9481 atcgcgcagg tgaagaagtt ccgctcggcg cgcgatgccg cgaaggtcga ggcggcgctc
     9541 gccgcggtga aggccgcgct cgccgacgac aagcagaatg tgatgcagcc gatcttcgag
     9601 gccgtgaaag cgaacgcgac gctcggcgag atctgcgatt tgttccgcac cgagctcggc
     9661 gagtaccgcg accccgccta tctctagcga gtcggtgagt accgcgctgc gtggcgccct
     9721 tctagcgagg cgagcgcgcc ttacttcgcg gctggatcgc gcttcttgat ggcgtcgatc
     9781 gttttgtgca tgccgcggcg aacgcctttg tcgctggcct tcggcagctg ctcggcgagg
     9841 aacttcgcgt cctcggcggt gcctttgctg gtgaacagcc gaagcgcgtc gtggtgccag
     9901 accgcgagca ggtacttctg attgcggccg ggatttttgg accgcttgtc ggcggcggcc
     9961 cggagccacg cgtcgcgaat tgcgggaagc gcgcgggcgt cgtccatgaa cgcgtacatg
    10021 ccgaagctgt ggccttcgaa ggcgtcgatc gggaacagct tcgcgaactg gacgaagagg
    10081 tcgagcttgc cgatcgtttt cgcgccgact tcgaggctcc acacgatgca gtcggag
//
LOCUS       DATLBZ010000293         4823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_326834, whole genome shotgun sequence.
ACCESSION   DATLBZ010000293 DATLBZ010000000
VERSION     DATLBZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326834"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VMZ53_15215"
     CDS             complement(<1..344)
                     /locus_tag="VMZ53_15215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HUS29853.1"
                     /translation="MERKGAIRARDASRKLIEPDDALNALARRVLEAAIEVHRYLGPG
                     FVESVYEQSLAIELGARSIKFEQQIRFPVLYKDTVVGTSQLDFLVEERLVVELKAVEE
                     LRVVHRQQLFSY"
     gene            435..1031
                     /locus_tag="VMZ53_15220"
     CDS             435..1031
                     /locus_tag="VMZ53_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29854.1"
                     /translation="MRFVILVVGIVLGIAGTVAYGMFAATDPVPVARPVPTHAPMTVT
                     LDESFLTALMQRSLSGSGSNAVPGVDVEKTRVRAELRGDQIIVHASVEVLGAPTEGTV
                     AMRPVLRDGRIVLDVVETNLGSIALPAMDQLLDKQINDRIHSLLDGMPVTITALGIDP
                     VLRSLVLSCQVDLERLEREGIPAAAAPAPAAAAPQAAR"
     gene            1205..2356
                     /locus_tag="VMZ53_15225"
     CDS             1205..2356
                     /locus_tag="VMZ53_15225"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUS29855.1"
                     /translation="MKNASQLLIAFVAVGVFQLAPKDAAADSHAAAVLDSNAIADVAE
                     KMVDSVVNISTESVIENEYEDPFSQFFGGPSGPMKAEGKGSGVIVTASGRILTNAHVV
                     NGATHITVTLQDGTELPAKVVGKDTRADLAVIQLDGKVPALKPIPWGDSSSLRLGEIV
                     LAIGDGLGVGKSVSMGIVSAKGRGLRIAEYEDFIQTDAAINPGNSGGALVNLKGELVG
                     INTAIASRSGGYQGIGFAIPTNMAKPIMEQLMKDGHVSRGYLGVGIATVNAALATEYQ
                     LPAQKGVLVASVEDGSPADKAGLIAGDIVTAINGAAVRTDDALKNTIAAIKPGTSIDL
                     DVIHKDGKTASVKAKLAELPEQSRASIAPQIKKPAAKAKSKRMLTPLTP"
     gene            2374..2745
                     /locus_tag="VMZ53_15230"
     CDS             2374..2745
                     /locus_tag="VMZ53_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29856.1"
                     /translation="MVKHATFALLGTLLLVGCMKQAQQVMGPDPDAVGASSCREIVES
                     CDAQCTDPICVNKCTEQGTREAQPQHAALVDCAQRNSCMDRDCMEQNCPQEIQTCMGP
                     PAEEQSQPPEVTKESQTTSGQ"
     gene            2854..>4823
                     /locus_tag="VMZ53_15235"
     CDS             2854..>4823
                     /locus_tag="VMZ53_15235"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS29857.1"
                     /translation="MFTFPIEGQNDVPVGSRIVVTFSEKVTAGALGPCTASTGAFCVV
                     GPSGPVDAMPVVSEDGTSVSLQGDLAPGTTYDVFARSELAPFAENLPSGKLFSFTTRS
                     DRPRAAAPTLVSINGGDPAKVGASFRPMFETTTLRLLFSEPLDPRSIKMGAGTFELLD
                     TSGSAVPATIVSDGIHVSIDPKADLTADANYTLRLGNQIVDLGGQPLAPFNATIKPAK
                     SRSATPVLHVLKPAQQGDPGFGVSRSGAKVNEIVIDKPLIGRETSQFTPVSLVGEIGD
                     PKALGGPIAFTLRKGQRLATTPLDIKLGGQIPSGLKTGDVMIELLTDATGRLYRNPYQ
                     PAEQRPENANAPLYVDLSMDVAIFAKDPKGNAVMTQTVLGMQATGTAIGTDGALVIET
                     VSSMELGLLGVTKAPSNLVLSLFTEGPGPMPAADTEAPTLVTSNPGQSDEANVDAGIE
                     LTFSEPIDLDRARAGGIKLQDTAGGADVAAVIESHGAGVVVRPLTPLPYGKIYRVVLG
                     DIADVAGNKANIPNVSFTTPVLQSTGVPGSVVAIHPGVPCALTGGTASSPGRCAGGDD
                     TDDLYKPFTIGPNEPVEVVYSEPVRRTSVTRGMACNQGTFRVEETDAAGTCTAVVAGT
                     LIARERTMTFIPDAPWVMGKRYKLTLVSGTND"
BASE COUNT          877 a         1533 c         1596 g          817 t
ORIGIN      
        1 aagtacgaga agagttgttg tcgatgaacg acacgaagtt cttcgaccgc ctttagctcg
       61 accacgagcc tttcctccac gaggaagtcg agctgcgatg tgccgacgac ggtgtctttg
      121 tagagcaccg gaaaacgaat ttgctgctcg aacttgatgc tgcgtgcgcc gagctctatc
      181 gcgagcgact gttcgtacac gctctcgacg aagccgggac ccaggtagcg gtgcacctcg
      241 atcgcggctt cgagtacacg tcgggcgagt gcgttcaggg cgtcatctgg ttcgatgagc
      301 ttccgacttg cgtcccttgc tctgattgct cccttgcgtt ccatccggcg gttatcggcc
      361 gctgcggccg gaggttgcgg cgagcgccgg tgtcacggac cggcgagggc attgcgattg
      421 caccactcgc aatcgtgcgc ttcgtaatcc tcgtcgtcgg aatcgttttg ggcatcgccg
      481 gcacggtcgc ctacggaatg ttcgcggcca cggatcctgt gccggtcgcg cgccctgtgc
      541 ccacgcacgc gccaatgacc gttactctcg acgaaagctt tctgacggcg ctcatgcagc
      601 gctcgctgtc gggaagcggt tcgaatgcgg tgcccggcgt agacgtggag aagacgcgag
      661 tgcgcgcgga gctgcgcggc gaccagatca tcgtgcacgc aagcgtcgaa gtactgggcg
      721 cgccgacgga gggcaccgtg gcgatgcgcc cggtcctgcg agatggccgc atcgtcctcg
      781 atgtggtcga gacgaacctc ggctcgattg cattgccggc gatggatcag cttctagaca
      841 agcagatcaa tgatcgcatc cactcgctgc tcgacggcat gccggtgacc atcaccgcgc
      901 tgggcatcga tccggtgctc cgcagcctcg tgctgtcatg ccaggtggac ctcgagcggc
      961 tcgagcgcga gggcattccc gctgcggctg cgccggcacc ggctgcagcg gcgccgcagg
     1021 ccgcccgcta agtacgcgtc tgagggagat tccgatcagg tggcggcggc tgcgtgatct
     1081 cgctgtcagc gattggcagc ggagggcgtg tctgaacagg tggttagctc gtgggaatgc
     1141 aggcaaccac ttgcgcggtc caaggttcgt aaccaacggg ccaagtgaga ggaccgagcg
     1201 acctatgaag aatgcctccc agttgctgat cgcctttgtc gcggtcggcg tgttccaact
     1261 cgcgccgaag gatgccgctg cggattcgca tgcggcggcg gtgctcgata gcaacgcgat
     1321 cgcggatgtc gccgagaaga tggtcgacag cgtcgtgaat atctcgaccg agagcgtgat
     1381 cgagaacgag tacgaagatc cgttctcgca gttcttcggc ggtccgtcgg gaccgatgaa
     1441 ggcggaaggg aagggctctg gcgtcatcgt gacggcgagc ggacgcatcc tgacgaacgc
     1501 gcatgtcgtg aacggcgcga cgcacatcac ggtcacgttg caggacggaa cggagttgcc
     1561 cgcgaaggtt gtgggtaagg acacgcgcgc ggacctcgcg gtcattcagc tcgatggaaa
     1621 agttccggcg ctcaagccga ttccgtgggg cgatagctcg tcgttgcggc tcggcgagat
     1681 cgtgctcgcg atcggcgatg gattgggcgt cggcaagagc gtgtcgatgg gcatcgtctc
     1741 ggcgaaaggc cgcggcctgc gcatcgcgga gtacgaggac ttcatccaga ccgacgcggc
     1801 gatcaatcca ggtaactcgg gcggcgcgct cgtgaacctc aagggcgagc tcgtcggcat
     1861 caatacggcg atcgcgtcgc gcagtggcgg ctatcagggc atcggcttcg cgatcccgac
     1921 gaacatggcc aagccgatca tggagcagct catgaaagat gggcacgtct cccgcggcta
     1981 cctcggcgtc ggcatcgcga ccgtcaacgc agcgctcgcg acggagtatc agttgcccgc
     2041 gcagaagggc gtgctcgttg cgagcgtcga ggatggcagc cccgcggaca aggcgggcct
     2101 catcgccggc gacatcgtca cggccatcaa cggtgctgcg gtgcgaacgg acgacgcgct
     2161 gaagaacacg atcgcggcga tcaagcccgg cacctcgatc gacctcgacg tgattcacaa
     2221 ggacggcaag acggccagcg tgaaggcgaa gctcgcagag ctgcccgagc agagccgggc
     2281 gtcgatcgcg ccgcagatca agaagccggc cgcgaaggcg aagtccaagc gcatgctcac
     2341 gccgctgacg ccttgagctg ataggctcgg cgcgtggtga agcacgcgac ctttgcactt
     2401 ctcggcacgc tcctgctcgt cggttgcatg aagcaggcgc agcaagtgat gggccccgat
     2461 ccggacgcgg ttggggcctc gtcgtgtcga gagatcgtcg agagctgcga cgcgcagtgc
     2521 accgatccca tttgcgtgaa caagtgcacc gagcagggca cgcgcgaggc ccagcctcag
     2581 cacgcggccc tcgtcgattg cgcacagcgc aacagctgta tggatcgtga ctgtatggag
     2641 cagaactgtc cgcaggagat ccaaacctgc atgggaccgc cagctgaaga gcagtcgcaa
     2701 ccgcccgagg tcacaaaaga atcacagacg acttcgggtc aatagctgta gtgtgggcga
     2761 gtcgtgcagc ggttcgtcct cctagcttcg gtcctttcgt tcgtcgcgtg cggtgactcc
     2821 accgagcccc tcgcgacagc acgacctggt gtcgtgttca cctttccgat cgagggacaa
     2881 aacgatgtcc ccgtcggttc acgcatcgtc gtcacgttct ccgagaaggt gactgcgggt
     2941 gcgctcgggc catgcaccgc gagcaccggt gcgttctgcg tcgtcgggcc gagcggtccg
     3001 gtcgatgcga tgccggtcgt cagcgaagac ggcacgagcg tgtcactgca aggtgacctc
     3061 gcgcccggta cgacgtacga cgtctttgcg cgcagcgagc tcgcaccatt tgccgagaat
     3121 ctgccgtcgg ggaaactgtt ctcgttcacg acgcgtagcg atcgcccgcg tgccgccgca
     3181 ccgacgctcg tgtcgatcaa cggcggtgat ccggcgaagg tcggcgcgtc gttccgaccg
     3241 atgttcgaga ccacgacgct gcggctgttg ttctccgagc cgctcgatcc gcgcagcatc
     3301 aagatgggcg ccggcacgtt cgagctcctc gatacgagcg gcagcgcagt gcccgcgacg
     3361 atcgtctccg atggcattca cgtctcgatc gatccgaagg cggacttgac cgcggacgcg
     3421 aactacacgc tgcgcctcgg caaccagatc gtcgatctcg gtggccaacc actcgccccg
     3481 ttcaacgcga cgatcaagcc tgcgaagagc cgctcggcca cgcccgtctt gcacgtgctg
     3541 aaaccggcgc agcaaggcga ccccggtttc ggcgtgtcgc gctccggtgc gaaggtcaac
     3601 gagatcgtga tcgacaagcc gctgatcggc cgcgagacgt cgcagttcac accggtgtcg
     3661 ctcgtcggtg aaatcggcga tccgaaagcg ctcggcggac cgatcgcatt cacgctgcgc
     3721 aaaggtcagc gcctcgcgac gaccccgctc gacatcaagc tcggcgggca aatcccgtcg
     3781 gggttgaaaa cgggcgacgt catgatcgag ctgctcaccg acgcgaccgg ccgcctctat
     3841 cgcaatccat atcagcccgc cgagcagcgg cccgagaatg caaacgcgcc gctgtacgtc
     3901 gacctctcga tggacgtcgc gatcttcgcg aaggatccga agggcaacgc cgtgatgacg
     3961 cagaccgtcc tcggcatgca ggcgaccggt acggcgatcg gcacagacgg cgcgctcgtg
     4021 atcgagaccg tgtcctcgat ggagctcggc ctgctcggcg tcaccaaggc gccatcgaat
     4081 ctcgtgctct ccctgttcac cgaaggtcca ggcccgatgc ctgcggccga caccgaagcg
     4141 ccgacgctcg tcacgagcaa tcccggccag agcgacgaag caaacgtcga cgcgggcatc
     4201 gagctcacgt tctccgagcc gatcgatctc gaccgcgcgc gcgcaggcgg catcaagctg
     4261 caggacaccg caggtggtgc cgacgtcgca gcggtgatcg agagccatgg cgctggcgtc
     4321 gtggtgcggc cgttgacgcc gcttccgtac ggcaagatct atcgcgtcgt cctcggtgac
     4381 atcgccgacg tcgcgggcaa caaggcgaac attccgaacg tcagcttcac gacgccggtc
     4441 ttgcagtcga caggcgtgcc tggctcggtt gtcgcgattc atcctggcgt gccgtgcgcg
     4501 ctgaccggcg gcaccgcgtc gagcccgggc cgctgcgcag gcggcgacga caccgacgat
     4561 ctctacaagc cgttcacgat tggcccgaac gagcccgtcg aggtcgtcta tagcgagccc
     4621 gttcgccgca cgtcggtgac ccgcggcatg gcgtgcaacc agggcacgtt ccgcgtcgaa
     4681 gaaaccgatg cagcgggaac gtgcacggcg gtcgtcgcgg gcacgctgat cgctcgcgag
     4741 cgcaccatga cgtttatccc cgacgcgccg tgggtaatgg gcaagcgcta caagctgacg
     4801 ctcgtctcgg gcaccaacga cag
//
LOCUS       DATLBZ010000294         4389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6124749, whole genome shotgun sequence.
ACCESSION   DATLBZ010000294 DATLBZ010000000
VERSION     DATLBZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6124749"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VMZ53_15240"
     CDS             complement(<1..1159)
                     /locus_tag="VMZ53_15240"
                     /inference="COORDINATES: protein motif:HMM:NF039639.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HUS29858.1"
                     /translation="MPSGWTRFVRAAFVLYLAATAAHIGWVVAHEPFTFDAWNVAVNS
                     GAKPITLERFFGFWRFEYTHSNPRIGQVLAYLAYKLEYFAVIATPLAYLAISLAVFVL
                     GTARLPSWRRSRDFMLWMLAIGYLWFALPQLGKTLFCRAYCANYIYAAAIQLWFVAVL
                     RLQPATCEGRASQRRCLAYALFGILAGMCNEHSGPTMIAFLLGYAWWLKRTTGKQPTL
                     VWSGALGAIIGFLAIFFAPGQGERYEGLGQRVTLFGRLLQRGVVGNVEILRDLLLAAA
                     PTIGLILVVVLLAKDDNREALRKALRFVVLAIVVSVAVAVTIFVSPKLGPRFFYVGCA
                     LLLAGFIGVADVALHRRRVLAPFVALAVIASIYAAARTVPLYGRVKKVSDSR"
     gene            complement(1178..2203)
                     /locus_tag="VMZ53_15245"
     CDS             complement(1178..2203)
                     /locus_tag="VMZ53_15245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUS29859.1"
                     /translation="MAPPVLSIVAPAYNEERNIPAFIAAIVPILESIGEPFEVIFVND
                     GSRDGTLGMLAAASSQDPRIKVVGLARNFGKDVALSAGLQHASGKAIIPIDCDLQHPV
                     ELIPQFVAKWREGFDMVIGVRSRRDEEGWLRRTLSQSYYKFMRSMTHIEIPPNAGDFR
                     LLDRKILDVINMMPERHRFMKGIFAWPGFKVASIEFQANIRANETRSSWSFFKLWRFA
                     LDGLFSFSSAPLKWWTYIGVLSAVGAFIYLVITLIQKLFFGIDAPGYASLLIVMLFFN
                     ALILISNGIQGEYIARIFEEVKGRPLYVIGQLYGFEQKPEVVTLPSSSSPSSSPPSGT
                     TQAKSMH"
     gene            2256..3428
                     /locus_tag="VMZ53_15250"
     CDS             2256..3428
                     /locus_tag="VMZ53_15250"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUS29860.1"
                     /translation="MRQAALALAILVGCGGNEVPPLPPACEVPVSGTSLTFRLIPNTR
                     GAIMIVTSPPRDLRKFAVDEGGVIKQMDDTGFTTTLLDISNQSDLAAGGEQGLLGLAF
                     HPQFATNGYFYIFYTTSNANVVDRYQISATDPNKADPATRTEILSIPDFAANHNGGMM
                     EFGSDGFLYIGTGDGGGGGDPNKNGQNQSSLLGKMLRIDVDHPANGKAYGIPKDNPYG
                     TEVFMMGLRNPWRWAFDPANGDMYIADVGQNEVEELDIVPAGKGKGLNFGWNMYEGDS
                     CYAPPCDAASKTMPQFTKTHGDSWCSIIGGAVYRGSCYPDIVGQYYYSDYCAHQLWAA
                     TKTGTTWTFSQPTVSYIDANGTHAGFPDAPTSLHPDSRGELFLTAQNGVYRLEAGQ"
     gene            complement(3385..>4389)
                     /locus_tag="VMZ53_15255"
     CDS             complement(3385..>4389)
                     /locus_tag="VMZ53_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29861.1"
                     /translation="PYISGVTGLIAKDPLMAGAILVGIACMLRSKNKLALAFLIFASL
                     LRWNALAATFAPMLLLFRWRELTGVRRYAIALVAWLGVTFAAYVANELLTDEREYLWY
                     WSYAYEDIGGTLEHVPEMDDATMERQLDGVPLVYHDRLHERFHKLYDPASHYHLMRNP
                     GHIFWIPKNQAERDAVAAAWKRIVLGNPDAYLKYRVDNFRLLLAIDRPPSFSNVYVWF
                     NVIAAPQVVEELGHDAGPSRIQAKLIDGSIWLSLTPLYYTFIYFALCLLLVPLVRERL
                     ELAVILSALGYELQWFFLAATADTRYSQWMAIATAVTAVLVARRLIARLRADTRRSAP
                     "
BASE COUNT          865 a         1355 c         1469 g          700 t
ORIGIN      
        1 tgcgcgagtc gctgactttt ttcacgcggc cgtagagcgg gacggtgcgc gcggcggcat
       61 agatcgatgc gatcacggcg agcgcgacga acggcgcgag gacgcggcgg cggtggagag
      121 cgacgtcggc cacgccgatg aagcctgcaa gcaggagcgc gcaaccgacg tagaagaagc
      181 gcggaccgag cttcggtgag acgaaaatgg tgacggcgac cgcgaccgac acgacgatcg
      241 cgagcacgac gaatcgcagc gctttgcgca gtgcctcgcg gttgtcgtct ttcgcgagga
      301 gcacgacgac gaggatgagc ccgatcgtcg gtgcggcagc gagcaggagg tcccggagga
      361 tctcgacgtt gccgacgacg ccgcgctgga gaaggcggcc gaacagcgtg acgcgctggc
      421 cgaggccttc gtagcgctcg ccctgccccg gcgcgaagaa gatcgcgagg aagccgatga
      481 tcgcgccgag cgcgccggac cagacgagcg tcggctgctt gccggtcgtg cgcttcagcc
      541 accacgcgta gccgaggaga aacgcgatca tcgtcgggcc gctgtgctcg ttgcacatgc
      601 cggcgagaat gccgaacagc gcgtacgcga gacagcggcg ttggctcgcg cgaccttcgc
      661 acgtggccgg ctggaggcgc agcaccgcga cgaaccacag ctggatcgcc gccgcgtaga
      721 tgtagttcgc gcagtacgcg cggcagaaca acgtcttgcc gagttgcgga agtgcgaacc
      781 acaggtagcc gatcgcgagc atccacagca tgaagtcgcg gctgcggcgc cagctcggca
      841 gcctcgcggt accgaggacg aacacggcga gcgagatcgc gagatacgcg agcggcgtcg
      901 cgatcacggc gaagtactcg agcttgtatg cgaggtacgc gaggacctgg ccgatgcgcg
      961 gattgctatg cgtgtactcg aagcgccaga acccgaagaa gcgctcgagc gtgatgggct
     1021 tcgcgccgct gttcaccgcg acgttccacg catcgaacgt gaatggctcg tgcgcgacga
     1081 cccagccgat atgcgctgcc gttgccgcga ggtacagcac gaacgcggcg cgcacgaagc
     1141 gagtccagcc cgacggcatg tcgccgtagg gatatcacta atgcatggat ttcgcctggg
     1201 tagtcccgct cggcgggctg ctactcggcg agctgctgct cggaagcgtc acgacctcgg
     1261 gcttctgctc gaacccgtac agctggccga tgacgtagag cggccggcct ttcacttctt
     1321 cgaagatgcg agcgatgtac tcgccctgaa tgccgttgct gatgaggatc agcgcgttga
     1381 agaacagcat cacgatcaag agcgacgcgt agccaggcgc gtcgatgccg aagaacagct
     1441 tctggatcag cgtgatgacc aagtagatga acgcgccgac cgcggagagc acgccgatgt
     1501 acgtccacca cttgagcggc gcgctcgaga acgagaacag gccgtcgagc gcgaaccgcc
     1561 acagcttgaa gaagctccac gagctgcggg tctcgttcgc gcgaatgttc gcctggaact
     1621 cgatcgaggc gaccttgaac cccggccacg cgaagatgcc cttcatgaag cggtgacgct
     1681 ccggcatcat gttgatcacg tcgaggatct tgcgatcgag caggcggaag tcgcccgcgt
     1741 tcggcgggat ctcgatgtgc gtcatcgagc gcatgaactt gtagtagctc tgcgagagcg
     1801 ttcggcgaag ccagccttct tcatcgcgcc ggctgcgcac gccgatcacc atgtcgaagc
     1861 cctcgcgcca cttcgcgacg aactgcggga tgagctcgac gggatgctgc aggtcgcaat
     1921 cgatcgggat gatcgccttg cccgatgcgt gctgcaggcc tgccgagagc gcgacgtcct
     1981 tgccgaagtt gcgcgcgagc ccgacgacct tgatgcgagg gtcctgcgag gacgcggcgg
     2041 cgagcatgcc gagcgtcccg tcgcggctgc cgtcgttgac aaagatcact tcgaacggtt
     2101 cgccgatgct ctcgaggatc gggacgatcg ccgcgatgaa cgcaggaata ttgcgctctt
     2161 cgttgtacgc gggggccacg atcgacaaca ccggcggagc cattgccacc ctcttatcac
     2221 agcacgctcc ggaccgaacg gcttatcctc ggagcatgcg gcaggcagca ctggccttgg
     2281 caattctagt aggttgcggc gggaacgaag ttcctccact gccgccggcg tgcgaggtgc
     2341 ccgtgagcgg gacgtcactg acgtttcgcc tcattccgaa cacgcgcggc gcgatcatga
     2401 tcgtcacgtc cccaccgcgg gacctgcgca agttcgccgt cgacgagggc ggcgtcatca
     2461 agcagatgga cgacacgggg ttcacgacga cgctgctcga tatcagcaat cagtcggacc
     2521 tcgcggcggg cggcgagcaa ggcctgctcg ggctcgcgtt tcatccgcag ttcgcgacga
     2581 acggctactt ctacattttc tatacgacgt cgaatgccaa cgtcgtcgat cgctatcaga
     2641 tcagcgcgac cgatccgaac aaagccgacc ccgcgacgcg caccgagatc ctgtcgattc
     2701 ccgacttcgc cgcgaaccac aacggcggaa tgatggagtt cggctccgac ggctttctgt
     2761 acatcggcac cggcgatggc ggcggcggcg gcgatccgaa caagaacggt cagaatcaga
     2821 gctcgctgct cggcaagatg cttcgcatcg acgtcgacca tccggcgaat ggcaaggcgt
     2881 acgggatccc caaggacaat ccgtacggca ccgaagtctt catgatgggt ctgcgcaacc
     2941 cgtggcggtg ggcgttcgat ccggcgaacg gcgacatgta catcgccgac gtcgggcaga
     3001 acgaagtcga agagctcgac atcgttcccg ccggcaaggg caaggggctt aacttcggct
     3061 ggaacatgta cgaaggcgat agctgctacg caccgccgtg cgatgcggcg agcaagacga
     3121 tgccgcagtt cacgaagacg cacggcgaca gctggtgctc gatcatcggc ggcgccgtgt
     3181 atcgcggcag ctgctacccg gacatcgtcg gccagtacta ctacagcgac tactgcgcgc
     3241 atcagctgtg ggccgcgacg aagaccggca cgacgtggac gttcagccag ccgaccgtca
     3301 gctacatcga cgcgaacggc acgcacgcag gattcccgga tgcaccgacg agcctgcatc
     3361 ccgattcacg cggcgagctg ttcctcacgg cgcagaacgg cgtgtatcgg ctcgaagccg
     3421 ggcaatgagc cgccgcgcga cgagcacggc cgtaaccgcg gttgcgatcg ccatccactg
     3481 cgagtagcga gtatccgcgg tcgctgcgag gaagaaccac tgcagctcgt agccgagcgc
     3541 gctgaggatc acggcgagct cgaggcgttc gcgcacaagc gggacgagca gcaggcacag
     3601 cgcgaagtaa atgaacgtgt agtagagcgg cgtcaggctg agccagatgc tgccgtcgat
     3661 gagcttggct tggatgcggc tcggaccggc gtcgtgaccg agctcctcta caacttgcgg
     3721 cgcggcgatc acgttgaacc agacatagac attcgagaac gacggcggtc gatcgatcgc
     3781 gagcagcagg cggaagttgt cgacgcgata cttcaagtag gcgtccgggt tgccaagcac
     3841 gatgcgcttc cacgccgccg cgaccgcatc gcgctctgcc tggttcttcg ggatccagaa
     3901 gatgtgtccc ggattgcgca tcaggtggta gtggctcgcc ggatcgtaga gcttgtgaaa
     3961 tcgctcgtgc aggcggtcgt gatagacgag cggcactccg tcgagctggc gttccatcgt
     4021 cgcgtcgtcc atctcgggga cgtgctccag cgtgccgccg atatcttcgt acgcgtatga
     4081 ccagtaccag aggtactcgc gctcgtcggt gagcagctcg tttgcgacat acgcggcaaa
     4141 cgtgacgccg agccatgcga ccagcgcgat tgcatagcgg cgtacgccgg taagctcgcg
     4201 ccagcgaaat agcagcagca tcggcgcgaa tgtcgcggcg agtgcgttcc agcgcaggag
     4261 gctcgcgaag atcaggaacg cgagcgcgag cttgttcttg ctgcgcagca tgcacgcgat
     4321 gccgacgagg atcgcgccgg ccatcagcgg gtcctttgcg atgaggccgg tgacgccgga
     4381 gatgtacgg
//
LOCUS       DATLBZ010000295         7501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2567363, whole genome shotgun sequence.
ACCESSION   DATLBZ010000295 DATLBZ010000000
VERSION     DATLBZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567363"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..577)
                     /locus_tag="VMZ53_15260"
     CDS             complement(248..577)
                     /locus_tag="VMZ53_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29862.1"
                     /translation="MTNTIFKFAPGLALALTTLVAPVAFAETAPKAPAAAKTTDTKAA
                     DAKTETKTTDTKTTDTKTSKVASKTPAKSTAKTTTTKTTTAKTAKTKTADAKTAPKAD
                     AKPDATK"
     gene            735..2048
                     /locus_tag="VMZ53_15265"
     CDS             735..2048
                     /locus_tag="VMZ53_15265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS29863.1"
                     /translation="MATRSTNGTNGTGGPVSASRAAWLAVTVAALGYFVDIYDLILFS
                     MVRKPSLVGLGVCGKLETSSAACDEILKQHGIYLINMQMGGMLLGGILWGMLGDKRGR
                     LSVLFGSITLYSIANIANGMVHTVDSYAVWRLLAGIGLAGELGAGITLVSELMGKEKR
                     GIGTTVVAAFGVSGGLLAGIIGGAFPSIPHPEWRTAYFIGGGLGLALLVMRIGVAESG
                     MYKAAASDSGLSRGNFFRIFTSARRVRRFLAIIAVGIPVWFVIGIPVTFADAIGRALG
                     MTPPPAPATAAFCAYGGAALGGLASGLISHWIKSRKRTLGIFLASNVVAIAVWFTLGA
                     SSLTAFYIMSFVAGAATGYWSVFISMAAELFGTNMRATVTTSVPNFVRGSLPLLTLSW
                     KALQESMSLIAATVVVGAGALALAVAGLASLDETFAVDLDFHERD"
     gene            2230..2928
                     /locus_tag="VMZ53_15270"
     CDS             2230..2928
                     /locus_tag="VMZ53_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29864.1"
                     /translation="MAAAPLLLDATTAAAQPEVRDHRTAPPPAPPPSSPEVRDHRSGG
                     PSGSVRYRRRPGPRFMMPLKIDIGAAGANTSRGFAPGIGAAVGIHWASLSPQPTQTDV
                     GIGVFGAILGAPSDPNAMSDNNSAAYGGAYLELAHTLSHGDFWRTWASGRGEYLGSKA
                     FDQPDHVGFGASGRLSAELFISGVGIEPRGLFLGTYALGVYVEGGLRDTASDIGKIHV
                     SGGLTFRTPLVFSP"
     gene            complement(2925..3203)
                     /locus_tag="VMZ53_15275"
     CDS             complement(2925..3203)
                     /locus_tag="VMZ53_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29865.1"
                     /translation="MNDQLQDISVAELEHVTGGARGGGSWNPFGWLDNLYHSVVNHFG
                     SQIGGTKLAEKLYGRRTTDADRSRAQAAMKKFLVAGHKLPKGVPNIFG"
     gene            3483..3770
                     /locus_tag="VMZ53_15280"
     CDS             3483..3770
                     /locus_tag="VMZ53_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29866.1"
                     /translation="MLTDSFITLSSSDLNTVSGGEGGFDAFADRERARIAEPYKQIVC
                     AGAGVKGGQELAKGVYGPNATDTDKIRAAEVLKSYCLGGAQLPAAAPKTPF"
     gene            3782..4036
                     /locus_tag="VMZ53_15285"
     CDS             3782..4036
                     /locus_tag="VMZ53_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29867.1"
                     /translation="MSTNHATELSLDDLSTITGGFGLRSIYREAVKGVTAGIGGWKLA
                     NEMYGTPEHGAPWAEKKRAMSALKGYLDASDKLPSWAPNW"
     gene            4045..4326
                     /locus_tag="VMZ53_15290"
     CDS             4045..4326
                     /locus_tag="VMZ53_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29868.1"
                     /translation="MSFTSIPTDELAVVCGGGAAWDAYVKKQRAAVEPQYKTVVCTAT
                     GVKGGRQFATEVYGKDRTTSGDMIRAAETLKGVCMGGAHLPEQAPQSPF"
     gene            4383..5033
                     /locus_tag="VMZ53_15295"
     CDS             4383..5033
                     /locus_tag="VMZ53_15295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HUS29869.1"
                     /translation="MARFRGVLLDVDGTLVDSNDAHANAWVEALAKFGHDVSFARARQ
                     FIGMGGDRLVEEVTGLPRDTRANKRIGDARSEIFLEKWLPSVRPVQGSRALLLRLLAE
                     GYQYALATAAKSNELEPLLEIADIADIVPNRTTSSDVEHSKPDPDIIEAALGKLASVE
                     RHQAVMIGDTPFDVLASRQANVAFIGVTCGGWSAEALNGAIEIATGPAELAIRFPS"
     gene            5053..5208
                     /locus_tag="VMZ53_15300"
     CDS             5053..5208
                     /locus_tag="VMZ53_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29870.1"
                     /translation="MCLICVEIAKSKMTVREARAQLREMREGMDRDHLREVEAKLEEV
                     EKTEPKP"
     gene            5412..6290
                     /locus_tag="VMZ53_15305"
     CDS             5412..6290
                     /locus_tag="VMZ53_15305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS29871.1"
                     /translation="MTTASTAATTTTTSKPVKTGHIDANGVNYYYEIHGKGEPVLLLH
                     GGLGSIDMFQGVWLPALAKSREVIAVDLHGHGRTPLGNRTINYVDLGNDMATLLDKLG
                     YKQVDAIGYSMGAAVAFRLSIQHSDKVRRLALVSAGFSPDGFYPEMRPMQSRLTGAMA
                     PMMKDTPMYKSYVEVAPNPSEFPKLLDQMGELMRTEFNWADDVKKLQTPVILVWGDSD
                     MFRPEHMVEFYKLVGGGQRDAGWQREHMSKNRMAILPNVTHYDMCMSQALVPTVLPFL
                     DGNQGQASWAEQVAKQ"
     gene            complement(6292..>7501)
                     /locus_tag="VMZ53_15310"
     CDS             complement(6292..>7501)
                     /locus_tag="VMZ53_15310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29872.1"
                     /translation="RSTRTERVRFELPDLGSIQRLVSAPDGKLLVIGDEGIARLALNA
                     SGGNAGAANGVAAKPALDASFGQAGVARIELKNAELQAATIDAKGRVIVVGHITTMRR
                     AEDLLVVRLRPNGVLDASFGKEGIVTADFGSTDDRPSAVFAVPGGDIVVIGSHQQGKD
                     GERYEGYLVRLDAKGQRRDKVAFFDCIPKSREFVTRALRDGDGFVVAGYAYNATADDS
                     GVYIARVRENGTLDPAFGEAGVRRVRGGDLGRSVAWELATAKNGDFLLGGSSGEEIAK
                     YRGFVMRFDHRGALVPTWGTEGTAWPSEGVKWISMVVEDHDDGPGGGSGDGGGDDSGS
                     GGDAGGVIAVNQLDGSLTRFDAQGVVDRGFGSDGIVKGRADDDYDAGIALDNGDIVVA
                     GDKQLVVYAR"
BASE COUNT         1275 a         2478 c         2387 g         1361 t
ORIGIN      
        1 gacacgcccc cgtccggccc aaacccgtcg tcgccgccgc cgccatcgtc accgccaccc
       61 ggccggatat cggtaccgca ggcgccgagc atgctggcga ctaacgccgc ggcagcgccg
      121 cgcagaacaa aacgacgatc gacttcaacg acaggcaaat aacgaggcac gccctctaga
      181 ggagggcact tcgcgaatgt cgcaaacgtc gtaagtgcgc gttccgtcta aggctgccgc
      241 gcccggtcta cttggtggcg tcgggcttgg cgtccgcctt cggagcggtc ttcgcgtcgg
      301 cggtcttcgt cttcgcagtc ttcgcggtgg tcgtcttggt cgtcgtcgtc ttggcggtcg
      361 acttcgccgg cgtcttcgac gcgaccttcg aggtcttcgt gtcggtcgtc ttcgtgtcgg
      421 tcgtcttcgt ttcggtcttc gcgtcagcag ccttggtgtc ggtcgtcttt gcggcggccg
      481 gcgccttcgg cgcggtttcg gcgaacgcga ccggagcgac gagcgtggtg agggcgagcg
      541 cgaggcccgg agcaaatttg aaaatcgtgt tggtcatgcg ttcgctgtaa cgcgtggcgt
      601 ggagtgcggc agcgatttgt gttcgggcgt ttttcatcgg cgcaaatcgc taggggcgtt
      661 tcgccccgcg ttgacgcgac tgcacgttcg ggcgatttgc gtcgcgctcg tcattcgtgg
      721 ctaagatggc cgcgatggcg acgaggtcga cgaacgggac gaacggaacc ggcgggccgg
      781 tcagtgcatc gcgcgcggcg tggctcgccg tgaccgtcgc ggcgctcggc tacttcgtcg
      841 acatctacga tctgattttg ttctcgatgg tccgcaagcc cagcctcgtc gggctcggcg
      901 tgtgcggcaa gctcgagacg tcgtcggccg cgtgcgacga gattctcaag cagcacggca
      961 tctacctcat caacatgcag atgggcggca tgctgctcgg cggcattctg tggggaatgc
     1021 tcggcgacaa acgcgggcgg ctcagcgtgc tgtttggatc gatcacgctg tattcgatcg
     1081 cgaacatcgc gaacggcatg gtgcacaccg tcgattcgta cgcggtgtgg cggttgctcg
     1141 cgggaattgg cttggccggc gagctcggcg ctggcatcac gctcgtctcg gagctgatgg
     1201 gcaaagagaa gaggggcatc ggcacgaccg tcgtcgcagc gttcggcgtg tcgggcggat
     1261 tgctcgcggg aattattggc ggcgcgtttc cgagcattcc gcacccggag tggcgcacgg
     1321 cgtattttat tggcggtggg ctcgggctcg cgctcctcgt aatgcgcatc ggcgtcgcgg
     1381 agtcgggcat gtacaaggcg gccgcttcgg atagcgggct gtcacgcggc aacttcttcc
     1441 gcatcttcac gtcggcgcga cgcgtgcggc gattcctcgc gatcatcgcg gtcggcattc
     1501 cggtctggtt cgtgatcgga attcccgtga cgtttgcgga cgcgatcggc cgcgcactcg
     1561 ggatgacgcc accacctgca ccggcgacgg ccgcgttttg tgcgtatggc ggcgcggcac
     1621 tcggcggcct cgcgagtggg ctcatctcgc attggatcaa gtcgcgcaag cgcacgcttg
     1681 gcattttctt ggcgagcaac gtcgtcgcga tcgcagtgtg gttcacgctc ggcgcgagct
     1741 cgctgacggc cttctacatc atgagcttcg tcgcgggcgc ggcgaccggc tattggtccg
     1801 tcttcatctc gatggcagcc gagctattcg gcacgaacat gcgcgcgacc gtgacgacat
     1861 cggtgccgaa cttcgtgcgc ggctcgctgc cgctgttgac gctcagctgg aaagcacttc
     1921 aggagagcat gtcgctcatc gcggcaacgg ttgtggttgg cgcaggcgca ttggcgctgg
     1981 cggtcgctgg tttggcgtct ctcgacgaga cgtttgccgt cgacctcgac tttcacgagc
     2041 gcgactgacg gtccgaccgc attgggccgc attgggccgc accacaccgc cgttaacaga
     2101 ggttcaccgt ttgccgggca accgcacgtg gtgtcaggga gttgcgcgtg gcacgcggag
     2161 tggatttact ggaccgaaca tctatttgag gtgcatgtga ccctagctcg ccttgtttgc
     2221 ttagccggag tggccgccgc gccgcttctc ctcgacgcca ccaccgcagc cgctcaacct
     2281 gaagttcgcg atcaccgcac ggcgcctccg cctgcaccgc cgccttcgtc gcccgaggtt
     2341 cgcgaccatc gaagcggtgg accgtcgggc agcgttcgct atcgcaggcg tccgggaccg
     2401 cgcttcatga tgccgctcaa gatcgacatt ggtgcggcgg gtgcgaacac gtcgcgcggc
     2461 tttgcgcccg gcatcggcgc cgcggtcggc attcactggg catcgctgtc accgcaaccg
     2521 acgcagaccg atgtcggcat cggtgtgttc ggcgcgatcc tcggtgcgcc ttccgatccg
     2581 aacgccatga gcgacaacaa cagcgcggcg tatggcggcg cgtatctcga gctcgcgcac
     2641 acgctctcgc atggcgactt ctggcgcacg tgggcatctg gccgcggcga gtacctcggc
     2701 agcaaggcat tcgaccaacc cgatcacgtc ggcttcggtg cgtcaggtcg actcagcgca
     2761 gagctgttca tcagcggcgt tggcatcgag ccgcgcggct tgttcctcgg cacgtacgcg
     2821 ctcggcgtct atgtcgaagg cggtctgcgt gacaccgcat ccgacatcgg caagatccac
     2881 gtcagcggtg gcctgacgtt ccgcacgccg ctcgtgttct ctccttaacc gaaaatattc
     2941 ggcacgccct tcggcagctt gtggccggcg acgaggaact tcttcatcgc tgcctgcgcc
     3001 cgcgagcggt cggcgtcggt cgtccggcgt ccgtacagtt tctcggcgag cttcgtgcca
     3061 ccgatctgcg atccgaaatg attgacgacg ctgtgataga ggttgtcgag ccagccgaac
     3121 ggattccagc tgccgccacc gcgcgcgccg ccggtcacgt gctcgagctc tgcgaccgag
     3181 atgtcctgta gttgatcatt catggcgctg gcgcatcgtg cagcgcgagt gccaagcggc
     3241 aagccgcgaa actacatcga tcctcgacga gagaagccgg ctctcggcgt ggccacgatc
     3301 gcagtgcagt tgccgtcaac tcgagcactc gacgtgtcgc gatcgaggag gttcgcgagc
     3361 ttgcgcggtc cagcctgcgt cctcgcggct acaagccgtc aaccgagggt cgcgagtgat
     3421 gcctggcatt tgcgctgcac ttgtcgtcga gcagcttcca acctccaaac aaggaatcag
     3481 ccatgctcac cgactcgttc atcacgctct cttcttcgga cctcaacact gtttccggcg
     3541 gcgaaggtgg cttcgacgca ttcgcggatc gcgagcgcgc tcgtatcgcc gagccgtaca
     3601 agcagatcgt ttgcgcaggc gccggcgtca aaggcggcca ggagctcgcc aagggcgtct
     3661 acggtccgaa cgcgacggac accgacaaga tccgcgcagc tgaagttctc aagagctatt
     3721 gcctcggtgg cgcgcagttg ccggccgctg ctcccaagac gccgttctga tccacagcgt
     3781 catgtccacg aatcatgcga ccgagctctc gctcgacgat ctctcgacga tcaccggagg
     3841 ctttggcctg cgttcgatct atcgcgaagc cgtcaaggga gtcaccgccg gcatcggcgg
     3901 ctggaagctc gcaaacgaga tgtacggaac gcccgagcac ggcgcaccgt gggccgagaa
     3961 gaagcgcgcg atgagtgcgc tcaagggcta tctcgatgcg agcgacaagc tgccgagctg
     4021 ggcacccaac tggtagaggc gaccatgtca ttcacatcca ttccaaccga cgagctcgcg
     4081 gtcgtctgcg gcggcggcgc tgcgtgggac gcttacgtca aaaagcagag agcagccgtc
     4141 gagcctcagt acaagaccgt cgtctgcacg gcgacgggcg tcaaaggtgg ccgtcaattc
     4201 gcgacggagg tctacggaaa agaccgcacg acgagcggcg atatgatccg cgcggccgaa
     4261 acgctgaagg gcgtttgcat gggtggcgcg cacttgcccg aacaggctcc gcaaagtccc
     4321 ttctgagtca taccgacgtg agttcgcgcg ccgcgcgggg cgtagagagc tacggtttct
     4381 gcatggcgag atttcgcgga gtgcttctcg acgtcgacgg aacgctggtc gacagcaatg
     4441 atgcgcacgc gaacgcctgg gtcgaagcgc tcgcaaaatt cggccacgac gtgtcgttcg
     4501 ctcgcgcgcg ccaattcatc gggatgggcg gcgatcgcct cgtcgaagaa gtgaccggcc
     4561 ttccgcgcga cacccgcgcg aacaaacgca tcggcgatgc gcgatcggag atcttcctcg
     4621 agaagtggtt gccgagcgtg cggccggtgc aaggctcgcg cgcgctgctg ctgcgcctcc
     4681 tcgcggaagg ctaccaatac gcgctcgcga cggccgcgaa gagcaacgag ctcgagccgc
     4741 tactcgagat tgcagacatc gcagacatcg tcccgaatcg cacgacctcg agcgatgtcg
     4801 agcacagcaa gcccgacccc gacatcatcg aagccgcgct cggcaagctc gcttccgtcg
     4861 agcgtcatca agcagtgatg atcggcgaca cgccattcga cgtgctcgcg tcgcgccaag
     4921 caaacgtcgc cttcatcggc gtcacgtgcg gcggctggtc cgccgaagct ctcaatggtg
     4981 cgatcgaaat cgcgacgggc ccggccgagc tcgcgatccg cttcccctcg taggacctgc
     5041 taaggtttgc ccatgtgcct catctgcgtg gagatcgcga agtcgaagat gaccgtccgc
     5101 gaggcgcgcg cccagctccg cgagatgcgt gaaggcatgg atcgcgatca cctgcgcgag
     5161 gtcgaagcga agctcgaaga agtcgagaag accgaaccga agccgtagct gcgtgttgcg
     5221 ctaagtgcgc gagcacgctg gccgaaatgg aaagtgcgta cgccgtgtcg attcggcgat
     5281 tcgccgttcg tcgtgtttgc atgaacgcga caaacgccat tcccagcatc ttgctcacca
     5341 tcctggctct cgctgctgcg tcgtgcgcgg ccgatcgcaa ggagccgcaa acctctcaca
     5401 aggcagctac cgtgacgacc gcatcgaccg cggcgaccac caccaccacg tccaagcccg
     5461 taaagaccgg ccacatcgac gccaacggcg tcaactacta ctacgagatc cacggcaagg
     5521 gagagccggt gctcctgctg cacggcggcc tcggatcgat cgacatgttc caaggcgtgt
     5581 ggctgccggc gctcgccaag tcgcgcgagg tgatcgcagt cgatctacac ggccacggcc
     5641 gcacgccgct cggcaatcgc acgatcaact acgtcgacct gggcaacgac atggcgacgc
     5701 tgctcgacaa gctcggctac aagcaagtcg acgcgatcgg ttactcgatg ggcgccgcgg
     5761 tcgccttccg cctctcgatc caacacagcg acaaagttcg ccggctcgcg ctcgtctccg
     5821 cgggcttctc gcccgacggc ttctatccgg agatgcgtcc catgcagtcg cggctcacgg
     5881 gtgcgatggc gccgatgatg aaggacacgc cgatgtacaa gtcgtacgtc gaggtcgcgc
     5941 cgaatccgtc cgagttcccg aagctcctcg atcaaatggg cgagctgatg cgcaccgaat
     6001 tcaactgggc cgacgacgtc aagaagctcc agacgcccgt catcctcgtg tggggcgaca
     6061 gcgacatgtt ccgccccgag cacatggtcg agttctacaa gctcgtcggt ggtggtcagc
     6121 gcgatgcagg ttggcagcgc gagcacatgt cgaagaaccg catggcgatc ctgcccaacg
     6181 tcacgcacta cgacatgtgc atgtcgcaag cgctggtccc gacggtcctg ccattcctcg
     6241 acggaaatca gggccaggcg agctgggccg agcaagtcgc gaagcaatag ctcatcgcgc
     6301 gtagacgaca agctgcttgt cgccggcgac tacgatgtcg ccgttgtcga gcgcgatgcc
     6361 cgcatcgtag tcatcatcgg cacgaccctt cacgatcccg tcgctgccga agccacgatc
     6421 cacgacgccc tgcgcgtcga accgggtcag cgagccatcg agctggttca cggcgatcac
     6481 gccaccggcg tccccgccgc taccgctatc gtcgccgccg ccgtcaccgc tgccgccgcc
     6541 aggcccatcg tcgtgatctt cgaccaccat cgagatccac ttcacgcctt cgcttggcca
     6601 tgcggtgccc tcggtgcccc acgtgggtac gagcgcgcca cgatgatcga agcgcatcac
     6661 gaagccgcga tacttcgcga tctcctcacc gctggatcca ccgaggagaa agtcgccgtt
     6721 cttcgcggtg gccagctccc acgcgacgga gcgtccgaga tcgccgccgc gcacgcgacg
     6781 cacaccggct tcgccgaatg ccggatcgag cgtcccgttc tcgcgcacac gcgcgatgta
     6841 cacgccgctg tcgtctgccg tcgcgttgta cgcgtagcca gcgacgacga acccatcgcc
     6901 atcgcggagc gcgcgcgtga cgaactcgcg agacttcggg atgcagtcga agaaggcgac
     6961 cttgtcgcgg cgctgtccct tcgcgtcgag ccgcacgaga tagccttcgt accgctcgcc
     7021 gtccttgccc tgctggtgcg agccgatcac gacgatgtcg ccacccggca ccgcgaacac
     7081 cgccgacggt cgatcgtccg tcgagccgaa gtctgcagtg acgattccct ctttgccgaa
     7141 gctcgcatcg agcacgccgt tcggccgcaa gcgcacgacg agtaggtcct ccgcacgccg
     7201 catcgtcgtg atgtgcccga cgacgatcac gcggcccttt gcatcgatcg tcgccgcctg
     7261 gagctcggcg ttcttcagct cgatccgcgc gacgcccgct tgcccgaacg acgcgtcgag
     7321 cgctggcttc gccgcgacgc cgttggccgc gccggcattc ccgccgctcg cgttcagcgc
     7381 aagccgtgcg atgccttcat caccgatcac gaggagcttg ccgtcaggcg cgctgacgag
     7441 acgctgaatc gaacccaggt ccggcagctc gaaccgcact ctctcggtgc gcgtgcttcg
     7501 c
//
LOCUS       DATLBZ010000296         6661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2567616, whole genome shotgun sequence.
ACCESSION   DATLBZ010000296 DATLBZ010000000
VERSION     DATLBZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567616"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VMZ53_15315"
     CDS             <1..1386
                     /locus_tag="VMZ53_15315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUS29873.1"
                     /translation="ALRKRAVALLQGLSVPDRGIALELIANLIHADGGISPAELQLQH
                     ELTAVVQKAARFGPNAKTIVEKKKSSPIVVEPAVWKDLVALTHPLLDPLEHTLSPHPV
                     ERQSQIEWDYRLLQAAMLQWDRQRRTGNGRLAGITDIGQLPAGSRFLDQFVHVMRPSA
                     PVELIVLGDLHGCYSCLKAALLQTNFVERVWAHQWDPANHPDVKLVLLGDYIDRGRFS
                     FDGVLRAALHLFVAMPDNVIVLRGNHEWWRWWQNNQISSAVYPAEGIASIAPYVPVEM
                     LEAYRVLFESMPTSFLCERMMFVHAGIPRDDTFAERYHDLSSLNDPEIRFQMMWSDPV
                     QADSIPVDIQRQNPRFEFGRAQYAEFMKRAGMNVMVRGHEQIDAGFEVFYDLGEHVLL
                     DLFSAGGHDNADLPIDSSYRTIRPMGLSIMHGEGTTPVATPWPLHYQPFNYAPHNGLY
                     RVQPVLEYRYL"
     gene            1440..2000
                     /locus_tag="VMZ53_15320"
     CDS             1440..2000
                     /locus_tag="VMZ53_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29874.1"
                     /translation="MGFRVGATWLLCIAFASATACSPLYGGKPEKLKTPEKKKKPKEA
                     EDAVVEVKYVDECQANFSDDPKKAQRQTPMSNQLFTAAETALDQSDKAKEDSMKVSLL
                     KEAIDKYRNALIKDPYNVPATLKLAVAYDRVLRKGCAIAMLKRLSALSNNPKWTSEAN
                     RNIDSIDANAQWFKGYRKEAMQAVGR"
     gene            2000..2611
                     /locus_tag="VMZ53_15325"
     CDS             2000..2611
                     /locus_tag="VMZ53_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29875.1"
                     /translation="MKKLAMIMFVLGASLGGCHEKQEVTAPPPPLRPDPEPKQEQTVA
                     KDCDPTDPASELKAISFDQRSIPEGMRLADQAYAKLNSANSAEVDRITREQYQADAVN
                     DFLTALAADPYNVKATYGLAAAYAKIGRKQCSINLLTRILQMRPHPSKKEEVERAIDK
                     LLGRKQALDGDFADMRRDSRFRELIAKMCEGTNDPNCVYGGDK"
     gene            2686..3546
                     /locus_tag="VMZ53_15330"
     CDS             2686..3546
                     /locus_tag="VMZ53_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29876.1"
                     /translation="MRTPALAALLTLALVPATASARPRPQPADAKAGGGPTGKSAPAC
                     GAKVLPLVVGNSWTYTSIAAPLPADEQIKRIAPGQPKKIVVTVKSVDKKDKDTVATLE
                     EKVTVERLKDGKPYDDEYSYESTITCNDKQFDISPGSFFFAGEPGGFFGLEITKTDRP
                     KGTSIQLTKGGIGEAQWGEDLIILWTRKPTEGSGAKLGSGKLELERRFTPQEPEPVTT
                     KQGMYRSEKIGLITTGRVTLDNPGTKELKPMELPANWLTTMWLADGVGVVQTLNMYAH
                     MYQLSEATLK"
     gene            3636..4814
                     /locus_tag="VMZ53_15335"
     CDS             3636..4814
                     /locus_tag="VMZ53_15335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HUS29877.1"
                     /translation="MKRVRWRVTKPLRDRVLAGHPWVYDRALAPPKGIKTGDLVTLVD
                     EKGPLATAIADPSSPLAARVLDLDPAATCDGAWARRRCEEAAARRSRDPLLVGCTGRR
                     LVHGEADGCPGLVVDAYTDTAVIVFDGPGVAEFWRARMADVLAGLERGGAMIEHVWLR
                     GQRGERMHGEALRGDPPDEIVINEDDARFGVDVRSGQKTGFFLDQRDNRRTIRKHAAG
                     ATVLNVFSYTGGFSIHAALGGATKVTSVDIAHPAIAAIERNVALSGLDIAAHERVAVD
                     AFDFFERAAKQGRTWDLVIVDPPSFAPNERSKTAALRAYERLATAALAVTSQGGRFAL
                     ASCSSHITEGDLLGVVANITTIGPTLRLRTAAGAGSDHPVLPAFSEGRYLKFLFFDKP
                     "
     gene            4866..>6661
                     /locus_tag="VMZ53_15340"
     CDS             4866..>6661
                     /locus_tag="VMZ53_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29878.1"
                     /translation="MRSSRWLLISALGSLFCTTVIATSPGCSDDAGNNATKAECEDGK
                     DNDGDGMTDFPDDLGCHAPDDLDNKEDDRPAPLCDDGRDNDGDGKHDFPNDPGCFAPN
                     QDDETDDCPNGALCPQCANGVDDDMNGQTDFPNDSGGCASASDPDEYTRNPVACGAAV
                     MIKPLPPDGHVAMGMLDSAKASSLTSMTCGGGGTEDVYELRVMQPKVIVATTDNPGTT
                     ADTVLYIRGQDCQNNSSELACQDDISTTVDRSTVTKAISTPGLYYLVVDAHDSASAGS
                     YELTVKFLTGEGVACGTADDCGPGLVCRIPKGQTAKVCAKHVCEDTTDEDGDGKNGFP
                     DDPGCVTAQDDDEADSCPGAGPNCAECGDGVDNDADGKTDFGTNGDTTCSAASSASEA
                     CVSTDGVQQIIAASTPGTTATGAVNDVKPACATSGTHTAADRTYRLDLPAMSDLTVDA
                     DSMTMDGVVALYNNTCGGTAIQCKDTPETITATNLAAGAYYFVVDGYSSGTGTYTIAV
                     SGHIQNGALCESTLTTAGAITCGVGYACKGTAGSKTCQPALCSDGVDNEASPDGKIDF
                     PFDPGCSDPADDTEADPTTLPICANGVDDDVPT"
BASE COUNT         1310 a         2335 c         2022 g          994 t
ORIGIN      
        1 gctctacgca agcgcgcggt tgcgctgctg caaggcctgt cggtgccgga tcgcggcatc
       61 gcgctcgagc tcatcgccaa cctcatccac gccgacggcg gcatctcgcc cgcggagctg
      121 cagctgcagc acgagctcac cgcggtcgtc cagaaggccg cgcgattcgg gccgaacgcg
      181 aagacgatcg tcgagaagaa gaagtcgagc ccgatcgtcg tcgagccggc ggtctggaaa
      241 gacctggtcg cgctcaccca tcctctgctc gatccgctcg agcacacgct gtcgccgcat
      301 cccgtcgagc ggcaatcgca gatcgagtgg gactaccgcc tgctgcaagc agcgatgctg
      361 cagtgggatc gccaacgccg aaccggcaac ggccgcctcg caggcatcac cgacatcggc
      421 cagcttcccg ccggctcgcg cttcctcgac cagttcgtcc acgtcatgcg gccgagcgcg
      481 cccgtcgagc tgatcgtgct cggcgatctg cacggctgct acagctgcct caaagcggct
      541 ttgctacaaa ccaacttcgt cgagcgcgtg tgggcgcatc agtgggatcc tgcaaaccat
      601 cccgacgtga agctcgtcct cctcggtgat tacatcgatc gcggacgctt ctcgttcgac
      661 ggcgtgctgc gcgcggcgct ccacctcttc gtcgcgatgc ccgacaacgt gatcgtgctg
      721 cgcggcaacc acgagtggtg gcgctggtgg cagaacaacc agatctcgag cgcggtgtat
      781 ccggccgaag gcatcgcatc gatcgcgccg tacgtgccgg tcgaaatgct cgaggcatat
      841 cgcgtgctgt tcgagagcat gccgacgtcg ttcctttgcg agcgcatgat gttcgtccac
      901 gcaggcatcc cgcgcgacga cacgttcgcg gagcgctacc acgatctatc cagcctgaac
      961 gatccggaga tccgcttcca aatgatgtgg agcgatcccg tgcaggccga ctcgatcccc
     1021 gtcgacatcc agcgccagaa cccgcgcttc gagttcggcc gcgcgcagta cgccgagttc
     1081 atgaagcgcg ctggcatgaa cgtgatggtt cgcggtcacg agcagatcga cgccggcttc
     1141 gaggtgttct acgacctcgg cgagcacgtg ctcctcgatt tgttctccgc cggcggccac
     1201 gacaacgccg atctgccgat cgattcgtcc tatcgaacga tccgcccgat gggcctatcc
     1261 atcatgcacg gcgagggcac gacgccggtc gccacgccgt ggcccctgca ctaccagccg
     1321 ttcaattacg cgcctcacaa cgggctctac cgcgtgcagc ccgtgctcga gtatcgatac
     1381 ctctaagtta gaagtttcgg gggtttgatt cgccgcgccg accgcgctac cgtcggcgta
     1441 tgggttttcg cgttggagca acctggctcc tctgcatcgc gttcgcgtcg gcgaccgcct
     1501 gctcgccgct ctacggtggc aagccggaga agctgaagac gcccgagaag aagaagaagc
     1561 cgaaggaagc ggaagacgcg gtcgtcgagg tcaagtacgt cgacgagtgc caagcgaact
     1621 tcagcgacga tccgaagaag gcacagcgcc agacgccgat gtcgaaccag ctgttcacgg
     1681 ccgcggagac cgcgctcgat cagtccgaca aggcgaagga agactcgatg aaggtcagcc
     1741 tcctcaaaga ggcgatcgac aagtatcgca acgcgctcat caaagatccg tacaacgttc
     1801 cggcgacgct caagctcgct gtcgcgtacg accgcgtgct tcgcaaaggc tgcgcgatcg
     1861 cgatgctgaa gcgtctctcc gcgctgtcga acaatccgaa gtggacgagc gaagcgaacc
     1921 gcaacatcga ttcgatcgac gccaacgcac aatggttcaa gggctatcgc aaagaagcga
     1981 tgcaagcagt gggacgctga tgaagaagct cgcaatgatc atgttcgtcc tcggcgcgag
     2041 cctgggcggc tgccacgaga agcaagaagt gacggcgccc ccgccgccgc tccgccccga
     2101 tcccgagccg aagcaggagc agacggtcgc caaagactgc gacccgaccg atcccgcgtc
     2161 cgagctcaag gcgatctcgt tcgatcagcg ttcgatcccc gaagggatgc gccttgccga
     2221 tcaggcgtac gcgaagctca acagcgcaaa cagcgccgag gtcgaccgca tcacccgcga
     2281 gcagtaccaa gcggacgcgg tcaatgactt cctcaccgcg ctcgccgccg acccgtacaa
     2341 cgtcaaagcg acgtacggcc tcgccgctgc ctacgcgaag atcggtcgca agcagtgctc
     2401 gatcaacctc ctcacgcgca tcctccagat gcggccgcac ccgagcaaga aggaggaggt
     2461 cgagcgggcg atcgacaagc tccttggccg caagcaggcc ctcgacggcg attttgcgga
     2521 catgcgccgc gacagccggt tccgggagtt gatcgccaag atgtgcgaag gcacgaacga
     2581 tccgaactgc gtctacggcg gcgataagta gccaccgcag ggtgtcagct catgcagcga
     2641 acgccgcttt acgcaggcgc attcgctagg tatgacttgg gcctcatgcg tactcccgcg
     2701 ctcgccgccc tgctcacttt ggccctcgtg cccgccacgg catcggcgcg tcctcgccca
     2761 cagccggccg acgcgaaggc cggtggtggc cccacgggca agtcggcccc tgcctgcggc
     2821 gccaaagttc tcccgctggt cgtcggcaac tcgtggacgt acacgtcgat cgccgcgccg
     2881 cttcccgcgg acgagcagat caagcgcatc gctccaggcc agcccaagaa gatcgtcgtc
     2941 acggtgaagt cggtcgacaa gaaagacaaa gacaccgtcg cgacgctcga agaaaaagtc
     3001 acggtcgagc gcctcaaaga cggcaagccg tacgacgacg agtacagcta cgagtcgacc
     3061 atcacgtgca acgacaaaca gttcgacatc tcgcccggca gcttcttctt cgccggtgag
     3121 cccggcggct tcttcggcct cgagatcaca aaaacggatc gccccaaagg cacgagcatc
     3181 cagctcacca agggcggcat cggcgaagcg cagtggggcg aagacctgat catcctctgg
     3241 acgcgcaagc ccaccgaagg ctccggcgcc aagctcggca gcggcaagct cgagctcgag
     3301 cgtcgcttca cgccgcaaga gcccgagccg gtgacgacga agcaaggcat gtatcgctcc
     3361 gaaaagatcg gcctcatcac cacgggccgc gtcacgctcg acaacccggg cacgaaagag
     3421 ctcaagccga tggagctgcc ggcgaactgg ctcacgacga tgtggctcgc cgacggcgtt
     3481 ggcgtcgtgc agacgctcaa catgtacgcg cacatgtatc agctgtcgga agcgacgctg
     3541 aagtaagcac cacgcacgcc acgcgctggc tgttcgcgca gaagtaagcg acgcgcgctc
     3601 gcgatgagcg gcgggcgctg aagtactctg tcgcgatgaa gcgcgtgcgt tggcgcgtta
     3661 cgaagccgct gcgcgatcgc gtgctcgcgg gccatccatg ggtctacgac cgcgcgctcg
     3721 cgccgcccaa aggcatcaag acgggcgacc tcgtgacgct cgtcgacgag aaaggcccgc
     3781 tcgcgaccgc gatcgccgat ccatcctcgc cgctcgctgc gcgcgtgctc gatctcgatc
     3841 cagctgcgac gtgcgatgga gcgtgggcgc gtcgtcgatg tgaagaagcc gccgctcgcc
     3901 gctcgcgcga tccactgctc gtgggctgca cgggaagacg cctggttcac ggcgaagccg
     3961 atggctgccc cggtctcgtc gtcgacgcgt acaccgacac cgctgtcatc gtgttcgatg
     4021 ggcccggcgt cgcggagttc tggcgcgcac gcatggcaga tgtcctcgca ggcctcgagc
     4081 gcggaggcgc aatgatcgag cacgtgtggc tgcgcggcca acgtggcgaa cgcatgcacg
     4141 gcgaagcgct gcgtggcgat ccgcccgacg agatcgtgat caacgaagac gacgcgcggt
     4201 tcggcgtcga cgtgcgcagc ggccagaaga cgggcttctt cctcgatcag cgcgacaacc
     4261 gccgcacgat ccgcaagcac gccgcaggcg cgacggtcct caacgtcttc tcgtacaccg
     4321 gtggcttctc gatccacgca gcgctcggtg gcgcgaccaa ggtcacgagt gtcgacatcg
     4381 cgcatccggc catcgcggcg atcgaacgca acgtcgcgct ctcgggcctc gacatcgcag
     4441 cgcacgagcg cgtcgcggtc gacgcattcg atttcttcga gcgcgccgcc aaacaaggcc
     4501 gcacgtggga cctcgtgatc gtcgatccgc catcgttcgc gccgaacgaa cgcagcaaga
     4561 ccgccgcgct ccgcgcctac gagcggctcg cgaccgccgc actcgccgtc acctcgcaag
     4621 gcggccgctt cgcgctcgcg tcgtgctcga gtcacatcac ggagggagat ctcttaggcg
     4681 tcgtggcaaa catcaccact attggtccga cccttcgtct ccggaccgct gcaggcgctg
     4741 gatctgacca tccggtgctc ccagctttca gtgaaggccg ttatctcaag ttcctgtttt
     4801 tcgataaacc ttgacccctc acctacactt ggttacagac gcgcgtccgg cactttggag
     4861 gcctcatgcg ctcgtctcgg tggctgctga tttcggctct tggctcgctg ttctgtacga
     4921 ccgtcatcgc gacgtcgccc ggctgcagtg acgacgcggg caacaacgcc acgaaggcgg
     4981 agtgcgagga cggcaaggac aacgacggcg acgggatgac cgacttcccg gacgacctcg
     5041 gctgccacgc gcccgacgac ctcgacaaca aggaagacga tcgtcccgcg ccgctgtgcg
     5101 acgacggtcg cgacaacgac ggcgacggca agcacgactt cccgaacgac ccgggctgct
     5161 tcgcgccgaa ccaagacgac gagaccgacg actgtccgaa cggcgcgctc tgcccgcagt
     5221 gcgcaaacgg cgtcgacgac gacatgaacg gccagaccga cttcccgaac gactcgggtg
     5281 gctgcgcgtc cgcatcggat ccggacgagt acacgcgcaa tcccgtcgcg tgcggcgcgg
     5341 ccgtgatgat caagccgctt ccgcccgacg gtcacgtggc gatgggcatg ctcgactcgg
     5401 cgaaggcctc ctctctcacc agcatgacct gcggtggcgg cggcacggaa gacgtctacg
     5461 agctgcgcgt catgcagccg aaagtcatcg tcgcgacgac cgacaatcca ggcacgacgg
     5521 cggacacggt cctctacatc cgcggccaag actgccagaa caactcgagc gagctcgcct
     5581 gccaagacga catctcgacg acggtcgacc gctcgacggt caccaaagcg atcagcacgc
     5641 cgggcctcta ctacctagtc gtcgacgcgc acgacagcgc gtccgccggc tcgtacgagc
     5701 tgaccgtcaa gttcctcacg ggtgaaggcg tcgcgtgcgg cacggccgac gactgcggcc
     5761 ccggcctcgt ctgccgcatc ccgaaaggcc aaacggccaa agtctgcgcg aagcacgtct
     5821 gtgaagacac caccgacgaa gacggcgacg gcaagaacgg gttccccgac gatccaggct
     5881 gcgtaacagc acaagacgac gacgaagccg actcctgtcc gggcgccgga ccgaactgcg
     5941 ccgaatgcgg tgacggcgtc gacaacgacg cagacggcaa gacggacttc ggcacgaacg
     6001 gcgacaccac gtgctccgcc gcctcctcag cctccgaagc ctgcgtctcg accgacggcg
     6061 tccaacaaat catcgcggcc tccaccccgg gcacgaccgc caccggcgca gtcaacgacg
     6121 tcaaaccggc gtgcgccacc tcgggcacgc acaccgccgc cgaccgcacg taccgcctcg
     6181 acctccccgc gatgtccgac ctcacggtcg acgcagactc gatgaccatg gacggcgtcg
     6241 tcgcgctcta caacaacacg tgcggcggca ccgccatcca gtgcaaagac acgcccgaaa
     6301 ccatcacggc caccaacctg gccgccggcg cgtactactt cgtggtcgac ggctactcct
     6361 cgggcacggg cacgtacacg atcgcagtca gcggccacat ccaaaacggc gcgctctgcg
     6421 aatccactct cacgaccgca ggcgccatca cgtgcggcgt cggctacgca tgcaaaggca
     6481 ccgcaggctc gaaaacctgc caacccgccc tctgctccga cggcgtcgac aacgaagcct
     6541 cgcccgacgg caagatcgac ttcccgttcg atcccggctg ctccgacccc gccgacgaca
     6601 ccgaagccga cccgaccacc cttcccatct gcgcgaacgg cgtcgacgac gacgtcccaa
     6661 c
//
LOCUS       DATLBZ010000297         9520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8363611, whole genome shotgun sequence.
ACCESSION   DATLBZ010000297 DATLBZ010000000
VERSION     DATLBZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8363611"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..2811
                     /locus_tag="VMZ53_15345"
     CDS             205..2811
                     /locus_tag="VMZ53_15345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUS29879.1"
                     /translation="MTWVVPLALGGFAVLLMAVFTMLGARKRHKAVMQPATTVGPAIL
                     VHDINDDRCTGCDACVAVCPTNVLDLVANKSRVLRFEDCIQCEACMFACPTEALVMFP
                     EGGVPPPLKVPEIDENFQTAVPGQYLIGEVAGKPLVKNAANLGRAVIEHMLTQGLYPG
                     ALGQMENTYDVAIVGSGPGGLSAALTCIQKGLSYVVLEKEQEIASTISRYPKGKLVMA
                     EPYDTSNVSFLPVFDSSKEQLIPIWKELLERVRLRVNQGESVESVQRVQDGTFEIRST
                     VAAYRAQRVVLSIGTRGKPRTLQVPGENLPKIFSLLEDPDVWAGRNVLVVGGGDSAVE
                     AALALSDAGARVIISYRGKGFNRAQPKNKSTVERYAAEGRIKAKLGSQVLQFDPEGVT
                     LALGDGQQKRYPNDAAFVLIGADPPVAWLEKMGVRFVERPHQYQMGKSDDIVRRFVAR
                     AADCPEDAARAAAAILGGSTGIEPSRAAAAMSLPMPMGDAKVSGPKKWLRSATSIFST
                     RTHSQMTPMPKAPQTNAVTGQGAGGKGKKLDAPMPLSEFAKRQRRADHTGHGRRDQLT
                     AGERTRILRMLRDEGGRIADEESQVHIGAAPPSPKGYDFDFDDENAAPPPPPSAPMRA
                     DVPAKPALVVGLAQARNKRQTGGPVRNKPVSVAPPAPPVPDMRQPLGDPRRPLEPMRS
                     KAPSMPPPVPGGRAAPRRTAPPPNPFHEKTRAMQDEELVNAVRSAPGPGRSLFDEPTR
                     MGDIDAHLLESTKRESALDHAPTGPDPEYPPKFLDAATELDSMFGKKHMQGGSNSFAN
                     NDDEATRMANVDVMAKQQKRPPARKQPPPPGVSSKRSPVPAHADDSTRAVDIRKDPNR
                     DPAMGDVDWDID"
     gene            2829..6188
                     /locus_tag="VMZ53_15350"
     CDS             2829..6188
                     /locus_tag="VMZ53_15350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29880.1"
                     /translation="MSGPTQGSDPPGRPSSPLADTIPASSPPQHSKEIEVDAPVSDRY
                     RLGAELGRGGMGRVVEAFDTQLGRTVALKEVLPTSSANVVRRFQREVQITARLEHASI
                     VPLYDSGTNTDGKPYYVMRRVTGQPLDQLIERARDLDDRLALLPNVLAAIDAIGHAHR
                     RGVIHRDLKPQNILVGELGETVVIDWGLAKVIGEEDIDPDSVEPRVPTAADSLKTQMG
                     AVFGTPGFMAPEQARGEELDQRGDVFALGATLYHLIAGKPPVSGKSATEMIASTMAHK
                     IVPITRLVPQTPPELVAIIDKALAADLAARYANAGDLAEDVRSFTTGQLVAAHNYTRW
                     QHMTRFARRHRGALVVAAAASVAVAVLAWVSVHRVLTERDAARDARAEAEVQRANAEA
                     KSVELRDRADALTLAHVRSLLDTNPTEAVAQLKHLDGSSPVVLAEAKALAKAAIMRGV
                     ARGLPSLPGMTVSFDMSADGKRVLQLTREGELQIVDIELGRAVSARAFGPGSSAAWVD
                     GGKRVLVFLDTQPPQLFDPVAGTVDTVGTSAFSSWAITQKGDLVAYTDDNDNLALLDI
                     KTRASTPLWTSGKVMRDIQIAADGSFIAFGEWNGPKTAHVLAIDRTGKVLVDHPGRAV
                     AFATSAKGKLAFSLYDEIDLVEPLAPSPTVKRVPLSKTDAQSVHGLRFNIDERLQMLG
                     FRNLTYYNGKDVWRSTTISDTLMSGKDAGMQTLVMNADDGAVHLLFGALHLPLGLTSR
                     PDTLIRIAAVPASTRIAATAGDAILVWDLAELVPRFLPMVAGQLVDEHHLLQMGGDSM
                     EEWNVHDLDSAAVQKIRLEPFGLVLGLDVADDGRILAMIDAIAEAGPPKRAIMIIAAD
                     RKTTSHVIDLTIARLIPGGVVFAREGGHLFGMATGATTPRELVRIDGEARSICATGPS
                     TYAVLSSAGELVKGTLDGAIDSRAHIDIVRNSFIACEPTAGEIIVASGNRLLRWSGTD
                     VTEVARFEAEPAGAIDSLSPTPTGLYIELANKAKLFIAAKGDRTPRPVPLSGPLGIAS
                     HGTVAAGLSTTGQVELVDLPSLAKWTLPKLITSRGSLNVSPDGTRLFQDSAVWRLPQA
                     GRDYGPWLDEVTNAELVDGRIVWPWQKPKTP"
     gene            complement(6189..7091)
                     /locus_tag="VMZ53_15355"
     CDS             complement(6189..7091)
                     /locus_tag="VMZ53_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29881.1"
                     /translation="MPWWTVYDDNHFVLRFDHDGVDPRPIPGRPVGAIRTRDIELDNG
                     VFEAMRCAWSGDSDRCGDRSIYAAGEQAHDAITEARAELRARTADAGADIVGDVRCFA
                     ERSVSAAEGSKLWCEGVAFAATSADAALDEVAREADPAAPIDHEPRVPEARFTVVADA
                     GVGMQGGKPIVSSTLGIRYRPIEVGFYIVDLQRESIGPRDNGAVGVGLTVLGRKQLRE
                     RSGADAILGGSAVAAFQNGSTNPDFDGLYQAFGGLAYQSRFNLWHGQPWVQLRAGAAT
                     GTAVKNKALPLLELHLGLATPERR"
     gene            7325..8464
                     /locus_tag="VMZ53_15360"
     CDS             7325..8464
                     /locus_tag="VMZ53_15360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HUS29882.1"
                     /translation="MLATQIRQKDSVFYFCAYPAEDLLDKVRFISRFYAEGAPSLPGE
                     GAEDNDEVAQFISKIESSDAAFQRQLGRAKVTAIKNFYETAVSQPPIPGTVLLFSEER
                     LKFDAVKPFNNVGNLAEPRGQYLIIDGQHRLAALHFFMQQRPDDAKTIHVPCVIFDGK
                     SEDFATEMFVIINSTPTRINKSHLIDLYEKVSWAAPDKKFAARVVDHLYRENDSPLRY
                     KINRLGGRSKQEKWILQAELFNEIHRWAMLDWKQIKKSSSWQRQAERYYEMVRDFLRA
                     AEKVFGDTWGHANYMVTRPVTIKALLRVVADLAAADPEPEDRVKRWTRKLAPWTDLAR
                     SFKQEGFYERFPAKGQVERVSRIYKELARSANIERRQKSGEDDDE"
     gene            complement(8497..>9520)
                     /locus_tag="VMZ53_15365"
     CDS             complement(8497..>9520)
                     /locus_tag="VMZ53_15365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HUS29883.1"
                     /translation="KFTGAAEQKPRWKRCVDRTTSGLRDVIAQMFVRDKFPGQSKEAA
                     EAQVHAISDAMSKNIDALAWMDATTKTKAKAKLAAMAYHIGYPKQWRTYDFKIDAKSW
                     GTNGLAARKAETQFQARKIGKPRDIDAWDIPASMVNAFYNPTHNKMVFPAGILQPPFY
                     SVDAAVQVNLGAMGMVVGHELTHGFDDEGSQFDAVGNMANWWQPETEKLFKERTQCVK
                     DQYSGYTISGVKLNGDLTAGENIADIGGIKLAFAAYRTLRSSAPETVVADGFTEDQQF
                     FLSTGQAWCAKERPEFEKMLAQVDPHSPPKWRINGSMADTPEFAKAFRCKLGAKMRPQ
                     KACVVW"
BASE COUNT         1652 a         3149 c         3161 g         1558 t
ORIGIN      
        1 ggaccatcga gatcctcccg cgagggggca aagtcatgcg taccggcgtg cccgtccggg
       61 tcgcgatcgg cgcgcccatc ccggtcgagg gtcgggacat cgagggactg atggcggaag
      121 tacgggattt cctggtgaac aacgtggaaa ggccccgtag ttgaaggcag cgaaaccacg
      181 taattcgggt atcctaattc ggctgtgacc tgggtcgtcc ccttggccct cgggggcttc
      241 gccgtcctcc tgatggcggt gttcaccatg ctgggtgccc ggaagcggca caaggcggtg
      301 atgcagccgg cgaccaccgt cggtccggcc atcctggtcc acgacatcaa cgatgaccgc
      361 tgcaccggct gcgatgcctg tgtcgcggtg tgcccgacga acgtcctcga cctcgtcgcg
      421 aacaagagcc gcgtgctccg gttcgaggac tgcatccagt gcgaagcgtg catgttcgcg
      481 tgcccgaccg aagcgctggt gatgttcccg gagggcggtg tacctccgcc gctcaaggtc
      541 cccgagatcg acgagaactt ccagactgcg gtgccgggcc agtacttgat cggcgaggtc
      601 gccggcaagc cgctcgtgaa gaacgccgcg aacctcggac gcgcggtgat cgagcacatg
      661 ctcacgcagg ggctctatcc tggcgcgctc ggccagatgg agaacacgta cgacgtcgcg
      721 atcgttggct ctggtccggg cggtctgtcg gcggcgctca cgtgtattca gaaggggctg
      781 tcgtacgtcg tgctcgagaa ggagcaggag attgcatcga cgatctctcg ctatccgaag
      841 ggcaagctcg tcatggccga gccctatgac acgtcgaacg tctccttctt gcccgtgttc
      901 gactcgtcga aagaacagct gattccgatc tggaaagagc tgctcgagcg cgtccgtctt
      961 cgcgtcaatc aaggcgagtc ggtcgagagt gtgcagcgcg tacaagacgg cacgttcgag
     1021 attcgctcga cggtcgctgc gtatcgcgcg caacgcgtcg tgctctcgat cggtacgcgc
     1081 ggcaagcctc gtacgctgca ggtccccggc gagaacctgc cgaagatctt cagcctgctc
     1141 gaggatccgg acgtgtgggc gggacgcaac gtgctcgtcg tcggcggtgg tgactccgcc
     1201 gtcgaagcgg cgctcgcgct ctccgatgcc ggcgcccgcg tgatcatttc gtatcgcggc
     1261 aagggcttca atcgcgccca gccgaagaac aagtcgaccg tcgagcgcta tgccgccgag
     1321 ggccgcatca aggcgaagct cggctcgcag gtgctgcagt tcgatccaga aggcgtcacg
     1381 ctcgcgctcg gtgacggcca gcagaagcgc tatccgaacg acgcggcctt cgtgctcatc
     1441 ggcgccgatc cgccggtcgc gtggctcgag aagatgggcg ttcgcttcgt cgagcgtccg
     1501 catcagtatc aaatgggcaa gagcgacgac atcgtgcgcc gcttcgtcgc gcgtgccgcg
     1561 gattgtccgg aagatgcagc gcgcgcagca gcagcgatcc tcggtggctc gacgggcatc
     1621 gagccgtcgc gtgcggccgc agcgatgtcg ctgccgatgc cgatgggcga tgcgaaggtc
     1681 agcggcccga agaagtggct gcgctccgcg acgagcatct tctcgacgcg cacccactcg
     1741 cagatgacgc cgatgccgaa ggcgccgcag acaaatgcgg tgaccgggca gggcgccggc
     1801 ggcaagggca agaagctcga cgcgccaatg ccgctgtcgg agttcgcgaa gcgccaacgc
     1861 cgcgcagacc acaccggtca cggccgccgc gatcagctca ctgccggcga gcgtactcgt
     1921 atcctccgca tgctccgcga cgaaggtggc cgtatcgccg acgaggaatc gcaagtccac
     1981 atcggcgccg cgccgccgag ccccaagggc tacgacttcg atttcgacga cgagaatgct
     2041 gcgccgccgc cgccgccgtc cgcaccgatg cgtgcagatg ttccggcgaa gcccgcgctc
     2101 gtcgttggtc tcgcgcaagc gcgcaacaag cgccagaccg gtggcccggt tcgcaacaag
     2161 ccggtgtcgg tcgcaccgcc cgcgccgccg gtgcccgata tgcgccagcc tctcggtgat
     2221 ccgcgccgcc cgctcgagcc gatgcgcagc aaagcgccgt cgatgccgcc gccggtccca
     2281 ggtggccgcg ccgcgcctcg tcgcaccgcg cctccgccga atccgttcca cgagaagacg
     2341 cgcgcgatgc aggacgaaga gctcgtcaat gcagtgcgct cggcgcctgg ccccggtcgc
     2401 tcgctgttcg acgagccgac gcgtatgggc gacatcgatg cgcacctgct cgaatcgacg
     2461 aagcgcgagt ccgcgctcga ccacgcgccg acgggtcccg acccggagta cccgccgaag
     2521 ttcctcgacg ccgccaccga gctcgactcg atgttcggca agaagcacat gcaaggcggc
     2581 agcaacagct tcgcgaacaa cgacgacgaa gcgacgcgca tggcgaacgt cgacgtgatg
     2641 gcgaagcagc agaagcgccc gcctgcgcgc aagcagcccc cgccgcctgg cgtctcgtcg
     2701 aagcggtcgc ccgtgcccgc ccacgccgat gacagcaccc gcgccgtcga cattcgcaag
     2761 gatccgaatc gcgatccggc gatgggcgac gtcgattggg acatcgacta ggctgctaga
     2821 atcgaggcat gtcggggcct actcagggaa gcgatccgcc tggtcgcccc agtagccccc
     2881 tcgccgatac gattccggcg agctcgccgc cgcagcacag taaagagatc gaggtagacg
     2941 cgccggtctc cgatcggtat cgcctcggtg cggagctcgg gcgcggcggc atgggccggg
     3001 tcgtcgaagc gttcgacacg cagctcgggc ggacggtcgc gctgaaagaa gtcctgccga
     3061 catcgagcgc gaacgtcgtg cggcggttcc agcgcgaagt tcagatcacc gcgcgcctcg
     3121 agcacgcgtc gatcgtcccg ctctacgact ccggcacgaa taccgacggc aagccctact
     3181 acgtgatgcg gcgcgtgaca ggacagccgc tcgatcagct gatcgaacgc gcacgcgacc
     3241 tcgacgatcg tctcgcgctg ctgccgaacg tgcttgcggc gatcgatgcg atcggacacg
     3301 cgcaccggcg cggcgtcatt catcgcgatc tgaagccgca gaacattctc gtcggcgagc
     3361 tcggcgagac ggtcgtgatc gattggggcc tcgcgaaggt gatcggcgag gaggacatcg
     3421 atccagactc cgtcgagccg agggtgccga cggcggcgga cagcttgaag acgcagatgg
     3481 gagctgtgtt cggcacgcca ggcttcatgg cgccggagca agcgcgcggt gaggagctcg
     3541 atcagcgcgg cgacgtgttc gcgctcggcg cgacgctgta tcacctgatc gcgggaaagc
     3601 cgcccgtgtc gggcaagagc gcgaccgaga tgatcgcgtc gacgatggcg cacaagatcg
     3661 tgccgatcac gcggctcgtt ccgcagacgc cgccggagct cgtcgcgatc atcgacaagg
     3721 cgctcgccgc cgacctcgcg gctcgctacg cgaacgccgg cgatctcgca gaagacgtac
     3781 gcagcttcac gaccggtcag ctcgtcgccg cgcacaacta cacgcggtgg cagcacatga
     3841 cgcggttcgc gcgccggcat cgtggcgcgt tggttgtcgc ggccgcagcg tcggtcgccg
     3901 tcgcggtgct cgcatgggtg agcgtgcatc gcgtgctgac ggagcgtgat gcggctcgtg
     3961 acgcgagagc cgaggccgag gtccagcgcg cgaatgccga ggcaaagtcc gtcgagctgc
     4021 gcgatcgcgc cgatgccttg acgctcgcgc acgtgcgctc gctgctcgac acgaatccga
     4081 ccgaagcggt cgcgcaactc aagcacctcg acggctcgtc gccggtcgtg ctcgccgaag
     4141 ccaaggcgct cgcgaaagct gcgatcatgc gcggtgtcgc gagaggcctg ccgagcttgc
     4201 ccggcatgac ggtgtcgttc gacatgtccg ccgacggcaa gcgcgttctc cagctcacgc
     4261 gtgaaggcga gctgcagatc gtcgatatcg agctcgggcg cgcagtctcg gcccgtgcat
     4321 tcggccccgg cagcagtgcg gcatgggtcg atggcggcaa gcgcgtgctc gtgttcttgg
     4381 acacccagcc gccgcagctg ttcgatcccg tcgcaggcac ggtcgatacc gtgggcacga
     4441 gcgccttctc gtcgtgggcg atcacgcaga agggcgacct cgtcgcgtac accgacgaca
     4501 acgacaacct cgcgctcctc gacatcaaga cgcgcgcctc gacgccgttg tggacgagcg
     4561 gcaaggtgat gcgcgacatc cagatcgcgg ctgacggcag cttcatcgcg ttcggcgaat
     4621 ggaatgggcc gaagaccgcg cacgtcctcg cgatcgatcg cacgggcaag gtgctcgtcg
     4681 atcatccagg gcgtgcggtt gcgttcgcga cctccgcgaa gggcaagctc gcgttctcgc
     4741 tctacgacga gatcgatctc gtcgaaccgc tcgcgccgtc gccgaccgtg aagcgcgtgc
     4801 cgctgagcaa gacggatgcg cagagcgtgc atggcctgcg cttcaacatc gacgagcggc
     4861 tgcaaatgct cgggtttcgc aacctgacct attacaacgg caaggacgtc tggcgcagca
     4921 cgacgatcag cgacacgttg atgagcggca aagacgcggg catgcaaacg ctcgtgatga
     4981 acgccgatga cggagcggtg cacttgctct tcggtgctct ccatctgccg ctcgggctga
     5041 catcgcggcc ggatacgctc attcgtattg cggcggtgcc agcgagcaca cgcattgcgg
     5101 ccaccgcggg tgatgcgatc ctcgtctggg atctcgccga gctggtgccg cggtttctgc
     5161 ccatggtggc ggggcagctc gtcgacgagc accacctgct gcagatgggc ggagactcga
     5221 tggaggagtg gaacgtccac gatctcgatt cggctgccgt ccagaaaatc cggctcgagc
     5281 cgttcggctt ggttctcggc ctcgatgtcg ccgacgacgg acgcatcctc gcgatgatcg
     5341 acgcgatcgc cgaggcaggc ccgcccaaac gcgcgatcat gatcatcgcg gccgatcgca
     5401 agactacgtc tcacgtcatc gatctgacga tcgcgcggct catcccgggc ggcgtcgtgt
     5461 tcgcgcgcga aggcggccac ctgttcggca tggcgacggg cgcaaccacg ccgcgcgagc
     5521 tcgtgcgcat cgacggcgag gctcgatcga tctgcgcgac ggggccgagc acctacgccg
     5581 tgctcagctc tgcgggcgag ctcgtcaaag ggacgctcga cggcgcgatc gacagccggg
     5641 cgcacatcga tatcgtgcgc aactcgttca tcgcgtgcga gccgaccgcg ggggagatta
     5701 tcgtcgcgtc gggcaaccgc ctcctgcgtt ggagcggcac ggatgtcacg gaggtcgcgc
     5761 gcttcgaagc ggagccggcg ggcgcgatcg attcgctctc gccgacgccg acgggcctct
     5821 acatcgagct cgcgaacaag gcgaagctct tcatcgctgc gaaaggcgat cggacaccgc
     5881 ggccggttcc tctcagcggc ccgctcggca tcgcctcgca tggcaccgtc gcggcgggcc
     5941 tctcgacgac gggccaggtc gagctcgtcg atctgccgtc gctggcgaag tggacgttgc
     6001 ccaagctgat cacgtcacgc gggagcctca acgtttctcc cgacggcacg cggctgttcc
     6061 aagactccgc cgtgtggcgc ctaccacaag cgggtcgtga ctacgggccg tggctcgacg
     6121 aggtcacgaa tgcagagcta gtcgacggcc gtatcgtatg gccgtggcaa aagcccaaga
     6181 cgccgtagtc agcggcgttc gggcgtcgcc aagccgaggt ggagctcgag cagcggcaac
     6241 gccttgttct tcactgcggt cccggtggcc gcgcccgcac gcagctgcac ccacggctgg
     6301 ccatgccaga ggttgaagcg cgactgatac gcgagcccgc caaacgcctg gtagaggccg
     6361 tcgaagtctg gattcgtcga gccgttctga aatgccgcga ccgccgatcc accgaggatc
     6421 gcgtctgcac cggagcgctc gcgcagctgc ttgcggccga gcacggtcag cccgacgccg
     6481 acggcgccat tgtcgcgagg cccgatgctc tcgcgctgta gatcgacgat gtagaagccg
     6541 acctcgatcg ggcgataccg aatgccgagc gtcgaggaga cgatgggctt accgccttgc
     6601 atgccgacac ccgcgtccgc aacgacggtg aagcgcgcct cgggtacacg cggctcgtga
     6661 tcgatcggcg cggccggatc cgcctcgcgc gcaacctcgt cgagggcggc gtccgcggag
     6721 gtcgcggcga acgcgacgcc ttcgcaccac agcttcgagc cttcagccgc actcacgctg
     6781 cgttccgcaa agcagcgaac gtcgccgacg atgtccgcac ccgcatcggc cgtgcgtgca
     6841 cgcagctcgg cgcgtgcttc cgtgatcgca tcgtgcgcct gctcgcccgc cgcatagatc
     6901 gagcggtcac cgcagcggtc tgagtcgcca ctccacgcgc accgcatcgc ttcgaagacg
     6961 ccgttgtcga gctcgatgtc gcgcgtgcga atcgcgccca ccggacgacc gggaatcggc
     7021 cgtgggtcga cgccgtcgtg gtcgaagcgc aagacgaagt ggttgtcgtc atacacggtc
     7081 caccacggca agaccttcat cctcacggcg acgctggagg gcgcgcgcgg cgggccgacc
     7141 ggcgtcagcg gagccggatg gcttgtccgg taccggggca tgcccggatt gccagcacat
     7201 gccgcacata cgaggaggag gcactgcagc agcttcacgc gtgtctgacc ccgcagatcg
     7261 ccaaatggct cgcggcaggg tgaggcaaac actcgacgtc aggaggctgg gataggatgc
     7321 gaccgtgcta gcgactcaga ttcgtcaaaa ggacagtgtc ttctacttct gcgcgtaccc
     7381 cgccgaagac ctgttggaca aggtgcggtt catcagccgg ttctacgcgg aaggcgcgcc
     7441 gagcttgcct ggtgaagggg cggaggataa cgacgaagtc gcgcagttca tctcgaagat
     7501 cgagtcgagc gatgctgcat ttcagcgtca gctcggccgc gcgaaggtga ctgcgatcaa
     7561 aaacttctac gagacagcgg tcagccagcc gccgattccc ggcacggtgc tgctgttctc
     7621 cgaagagcgg ctgaagttcg atgcggtcaa gccgttcaac aacgtcggca acctcgccga
     7681 gccccgcggc cagtacctca tcatcgacgg ccagcatcgc ctcgccgcgc tgcacttctt
     7741 catgcagcag cggccggatg acgccaagac gatccacgtg ccgtgcgtga tcttcgacgg
     7801 caagagcgag gatttcgcga cggagatgtt cgtcatcatc aactcgacgc cgacgcgcat
     7861 caacaagagc cacctcatcg atctctacga gaaggtgtcg tgggcggcgc ccgacaagaa
     7921 gtttgcggcg cgcgtcgtcg atcatctcta tcgcgagaac gattcgccgc tgcgctacaa
     7981 gatcaatcgc ctcggcggcc gcagcaagca ggagaaatgg atcctgcagg ccgagctctt
     8041 caacgagatc catcgctggg cgatgctcga ttggaagcag atcaagaaga gctcgagctg
     8101 gcagcgtcag gccgagcgct actacgagat ggtgcgcgac ttcctgcgtg cagccgagaa
     8161 ggtgttcggc gacacgtggg gccacgcgaa ttacatggtg acgcgcccgg tgacgatcaa
     8221 agcgctgttg cgcgtcgtcg cggatctggc cgcggccgat cctgagccgg aggatcgcgt
     8281 gaagcggtgg acgcgcaagc tcgcgccgtg gacggacctc gcccgcagct tcaaacagga
     8341 aggcttctac gagcgcttcc cggcgaaggg ccaagtcgag cgcgtttcgc gcatctacaa
     8401 ggagctcgcg cgctccgcga acatcgaacg ccgccagaag agcggcgaag acgacgacga
     8461 gtagccgtca gctgcgcgcg ttggatcgag gctgcgttac cagacgacgc aggctttttg
     8521 cggacgcatc ttcgcgccga gcttgcagcg gaatgccttt gcgaattcgg gcgtgtccgc
     8581 catcgagccg ttgatgcgcc acttcggcgg cgagtgcggg tccacctgcg cgagcatctt
     8641 ctcgaactcg gggcgctcct tcgcgcacca agcctggccc gtcgacagga agaactgctg
     8701 atcctccgtg aagccgtcgg cgacgaccgt ctcgggcgcg ctcgagcgca gcgtgcggta
     8761 cgcggcgaac gcgagcttga tgccgccgat gtccgcgatg ttctcgcccg cggtgagatc
     8821 accgtttagc ttcacgcccg agatcgtgta gccgctgtat tggtctttca cgcactgcgt
     8881 gcgctccttg aacagcttct cggtttcggg ctgccaccag ttcgccatgt tgccgacggc
     8941 gtcgaactgc gagccttcgt cgtcgaagcc gtgcgtgagc tcgtgaccga cgaccatgcc
     9001 catcgcgccg aggttgacct gaacggcggc atccacgctg tagaacggcg gctgcaggat
     9061 gccggccggg aacaccatct tgttgtgcgt cgggttgtag aacgcattga ccatcgacgc
     9121 tgggatgtcc cacgcgtcga tgtcgcgcgg cttgccgatc ttgcgggcct ggaattgcgt
     9181 ctccgctttg cgtgccgcga gcccgttcgt gccccagctc ttcgcgtcga tcttgaagtc
     9241 gtaggtgcgc cactgcttcg gatagccgat gtgataggcc atcgccgcga gcttggcctt
     9301 tgccttcgtc ttcgtcgtcg cgtccatcca cgcgagcgca tcgatgttct tgctcatcgc
     9361 gtcgctgatc gcgtgcacct gcgcttcggc ggcctccttg ctctggccgg ggaacttgtc
     9421 ccgcacgaac atctgagcaa tcacgtcgcg caggccgctc gtcgtgcgat cgacacagcg
     9481 cttccagcga ggcttttgct ccgccgcgcc cgtgaactta
//
LOCUS       DATLBZ010000298         6911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1080834, whole genome shotgun sequence.
ACCESSION   DATLBZ010000298 DATLBZ010000000
VERSION     DATLBZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080834"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VMZ53_15370"
     CDS             <1..544
                     /locus_tag="VMZ53_15370"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HUS29884.1"
                     /translation="LELATELGDEILLIHPPIVARPASAGKFQYDAGMLQVGEPMPDV
                     TLQGEAGPVRLKDRLGKALVVYFYPKDETYGCTIEACSFRDQYEDFVAAGADVIGVSR
                     DDATSHAGFKSHHRLPFTLLTDPGGKLADAWGVKTSFGIGGRVTFVFDKEGVCRHRFD
                     SKLRFGKHVDEALDVVKRLA"
     gene            672..1271
                     /locus_tag="VMZ53_15375"
     CDS             672..1271
                     /locus_tag="VMZ53_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29885.1"
                     /translation="MRCSVVVLALVLASSVASAQPGWNQFSPPGLTPPTVQLSPTPGG
                     PRKDPSTAVLLSLGITTASYITLFAAEDHEGLQLLAFAGTYFGPSTGQWYAGQVGGLG
                     LGLRAAGFVLALYGISQLLSEDDCEYDYDCSDSSADTGALAFWGGAGLWVGSTVYDIV
                     LARRAAESWNARHSVAIAPMVTADAGGNRTPGLVLTGRF"
     gene            complement(1268..1678)
                     /locus_tag="VMZ53_15380"
     CDS             complement(1268..1678)
                     /locus_tag="VMZ53_15380"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS29886.1"
                     /translation="MRLDHLVLWAEDPLKTVEWFERVVGLTAVRLDEFKAKQVMFPSV
                     RVSDDTIIDIMPKAAAPVVNAIPGAKGTAGHPTNHVCLAMSEAEYHALAKRLEESGAP
                     AGRFMENQYGARGHAPKAFYFRDPDGNILEARYY"
     gene            complement(1688..4543)
                     /locus_tag="VMZ53_15385"
     CDS             complement(1688..4543)
                     /locus_tag="VMZ53_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS29887.1"
                     /translation="MADQSDDATRQDKPGRVSQPSLRVQPDFGPRYRVIGIVGKGGMG
                     EVFRAYDTELKGEVALKIVRGDADHEEALTRFRREIALARKVTSPYVLRVYDISEHDG
                     LRFLSMEFVDGEDLAALMRREKQLPIDRAVRIFRQVCEGLVAAHAQGVVHRDLKPQNV
                     LVDKDGGVRVADFGLARSITESGLTASGAVLGSPAYMSPEQVKGDPTDERSDIYSLGI
                     MLYQLVTGETPFKADTPHAVMEMRLHKKPRLLRDVRPDAPAYLEGIAAKCLALNPSGR
                     YRTVKEVLGAVETGGVTTEPVRRVAAGRPTWLIPAIAAGALCAAGGVVAVLVISGGSK
                     KGASPNERPALIIASARGPEAGAGPVATTSPMTTLVLPLTNTTSETSLDGTVEIIVTS
                     SLRRSTVLDPITGASLRRVASAFGNDVAIDDKLAARIAERDGGRVLTVHPTAIERGAK
                     LQIGLTVTDSTGALVFEETVEAPALDAVAPVTARLASALRGTLGETVAESERDRSGLS
                     ASLDADREYTLGLAAKATGDYSGASAHLERAIAKDSGFALARQNLAIVYENMGRMTQS
                     RAQFERALASVDNMGERDRQKFLGDYYRQVTEEYDRAITHYKQLLAKWPKDLPAAANL
                     CLAYYYHGDRESLLEQARKTAKEHPKELIVRDNLASFEISVGNYDRAIDEELAVLAEF
                     PRVTHSVHINLAVAYTLSGKRDKALASVEKAKGLDASTGSAVAADLLAAEGRVREAIA
                     ELEKGLPADNKDNADNAELKQAMLARLYARQGNQAAARAAAAEVKNQPYPLLQAALVQ
                     LAVGDTKRALATASKFADDTAPSRRAYGRMIEAESLRLKGKPQQAMITLQEAIKLADT
                     PLPHVLAIRAALDAKRYPEAYSELQAAIARRGEIALGDDTVCELTLVSELSYLLAKVQ
                     EGLGSPDAAKSYKAFIDGLHDPDANDPLVADARKHLQ"
     gene            4625..5254
                     /locus_tag="VMZ53_15390"
     CDS             4625..5254
                     /locus_tag="VMZ53_15390"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HUS29888.1"
                     /translation="MRIAIIGSGNIGGGLGRAWKRKGHDVTFGARNPEDAELLALCNE
                     TGAHAASVSDSVRNADVIALAVPYAALDEVLAATGELDGKVVIDCTNAVERGAGGMQL
                     KYGHTTSSAEQLQARIPKAHVVRSFNAQGAENLANPSYGGVPASNFYCGDHADAKRVV
                     AQLVSDVGFEAVDAGPLASARYLEPLMLLWINVSRTVGSRDIAFKLLRR"
     gene            complement(5324..6772)
                     /locus_tag="VMZ53_15395"
     CDS             complement(5324..6772)
                     /locus_tag="VMZ53_15395"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HUS29889.1"
                     /translation="MTRDGMVKVEREVVAQHTQVDRALRRIAVAHAALDADEAHWLRI
                     AEQQRVWPKLGYVHAFEYLEEVFGYAPRTAKERLRVARELGDMPQLEDALRCGETSWS
                     VARELTRVATSDTIERWLDEARGRRLRDVEQMVAGRKKGDLPGDPADPARVKHRVVLE
                     LDGESFVVWRQMQQSIEIESDRKLDDRDLVLAIGRRILGEAGHRRREESLHAGGPAAG
                     APASGVEAAAAEAETTAAARTLPAPGAAAANGNVDTTVADKKAPLRSVVARYMIQLTT
                     CRDCGRGWQDGAGVRVEVDRSVVDRAQCNAVICDDEKGMRPKRTIPARIERLVLERAQ
                     HRCEVPGCRSSRYLAIHHIVFWSHGGTHELSNLIVLCDGHHRLLHDGLLKITGEAPDK
                     LAFERGGVPLAGREGHAPRKHVAAASVKRAMNAPDLERDDPATLAQAALRQLGFKAPL
                     AARAVDEACAHVGTGVELGVLIREALRHCGER"
BASE COUNT         1104 a         2449 c         2164 g         1194 t
ORIGIN      
        1 cctcgagctg gcgacggagc tgggcgacga gatcctgctt atccatcccc caatcgtcgc
       61 caggcccgcg agcgcgggca aatttcagta cgatgccggc atgctgcaag tcggcgaacc
      121 gatgcccgat gtaacgctcc agggtgaggc gggtcccgtt cggctcaaag accgcctagg
      181 caaggcgctc gtcgtctact tctatccaaa ggacgaaacc tacggctgca cgatcgaagc
      241 gtgcagtttc cgcgatcaat acgaggactt cgtcgccgcc ggcgctgacg tcatcggcgt
      301 ctcgcgcgac gacgccacgt cccacgccgg cttcaagtcc catcaccggt tgccattcac
      361 cctgctgacc gatccgggcg gcaagttggc cgatgcgtgg ggcgtcaaga cgagcttcgg
      421 gatcggcggc cgcgtgacat tcgtgttcga caaagagggc gtctgccgcc accgcttcga
      481 ctcgaagctg cgcttcggca agcacgtcga cgaagccctc gacgtggtca agcgcctcgc
      541 ctgacttcac gcgacggccg ttgccatggc gccgtggtca gggtcacacg catggcatca
      601 ggttttctga gggtcggccc cagcctcatc gcgcacttcc gcgcggcacg ccacttgtac
      661 tggccacccg catgcgctgc tccgtggttg tcctcgccct cgtgctcgcg tcatccgtcg
      721 cgtccgccca accgggctgg aaccagttca gccctcccgg cctgacgccg ccgacggtgc
      781 agctcagccc gactcccggc ggcccgcgca aggacccgtc gaccgcggtc ctcctgtcgc
      841 tcggcatcac gaccgccagc tacatcacgc tcttcgccgc cgaagatcac gaaggcctgc
      901 agctgctcgc cttcgccggc acgtacttcg gcccgagcac tggccagtgg tacgccggcc
      961 aagtcggtgg cctcggcctc ggcctgcgcg ccgctggctt cgtactcgcg ctctacggca
     1021 tctcgcagct cctctcggag gacgactgcg aatacgacta cgactgcagc gactccagcg
     1081 ctgataccgg cgcgctcgcc ttctggggcg gcgctggcct ctgggtcggc tcgaccgtct
     1141 acgacatcgt gctcgcacga cgagccgccg agagctggaa cgcccgccac agcgtggcca
     1201 tcgcgccgat ggtgacggcc gatgcgggcg gcaatcgcac gccgggcctc gttctcaccg
     1261 gtcgcttcta gtagtagcgc gcctcgagga tgttgccgtc ggggtcgcga aagtagaacg
     1321 ccttgggcgc gtggccgcgc gcgccgtact gattctccat gaagcggccc gccggcgcgc
     1381 cgctctcctc gagccgcttc gcgagcgcgt ggtattccgc ttcgctcatc gcgaggcaga
     1441 cgtgattcgt cgggtgtccc gcggttccct tcgcgccggg aatcgcattg acgaccggcg
     1501 ccgcggcctt cggcatgatg tcgatgatcg tgtcgtcgga gacgcgcacg ctgggaaaca
     1561 tcacctgctt cgctttgaat tcgtcgaggc gaacggcggt gaggccgaca acgcgctcga
     1621 accactcgac ggtcttcagc ggatcttcag cccagaggac gaggtggtcg aggcgcatgg
     1681 tggcagccta ctgcaaatgc ttgcgtgcat ccgcgacgag cggatcgttc gcatcgggat
     1741 catgcaggcc gtcgatgaac gctttgtatg actttgcagc gtcggggctg ccgaggcctt
     1801 cctggacctt cgcgagcagg tagctcagct cgctgacaag cgtgagctcg cagacggtgt
     1861 cgtcgccgag cgcgatttcg ccacgccgag cgatcgccgc ttgcagctcg ctgtacgcct
     1921 cggggtagcg cttcgcatcg agcgcggcgc gaatcgcgag cacgtgcggc agcggcgtat
     1981 ccgcgagctt gatcgcctct tgcagcgtga tcatcgcctg ctgcggcttg cccttcagac
     2041 gcagcgactc ggcctcgatc atgcgcccgt acgcgcggcg gctcggcgct gtgtcgtcgg
     2101 caaacttcga tgcggtcgcg agcgcgcgct tcgtatcacc gaccgcgagc tgaacgagag
     2161 ccgcctgcag caacgggtac ggctggttct tcacctcggc tgcggcagcg cgcgctgcgg
     2221 cctgattgcc ctgtcgtgcg tacaaccgcg cgagcatcgc ctgcttgagc tcggcattgt
     2281 cggcgttgtc cttgttgtcc gccgggagac ccttctcgag ctcggcgatg gcttcgcgaa
     2341 ctcggccctc cgccgcgagc agatcggcgg ccacggcgga tcctgtcgac gcgtcgagcc
     2401 ccttcgcttt ctcgaccgat gcgagcgcct tgtcgcgctt accgctgagc gtgtatgcga
     2461 ccgcgaggtt gatgtgcacc gaatgcgtga cgcgtggaaa ctccgccagc acggcgagct
     2521 cttcgtcgat cgcgcggtcg tagttgccga cggagatctc gaagctcgcg aggttgtcgc
     2581 gaacgatgag ctccttcgga tgctccttcg ccgtcttgcg agcttgctcg agcaagctct
     2641 cgcgatcgcc atggtagtag tacgcgaggc agaggttcgc ggccgcgggc agatccttgg
     2701 gccacttcgc gagcagctgc ttgtagtgcg tgatcgcgcg gtcgtactct tcggtgacct
     2761 gacgatagta atcgccgagg aacttctggc gatcgcgctc gcccatgttg tcgacgctcg
     2821 cgagcgcccg ctcgaactgc gcgcgcgact gcgtcatgcg gcccatgttc tcgtacacga
     2881 tcgcgagatt ctggcgggct agcgcaaagc ctgagtcctt cgcgatcgcg cgttcgagat
     2941 gcgccgacgc ccccgagtaa tcgccagtcg ccttcgccgc cagaccaagc gtgtactcgc
     3001 gatctgcgtc gagcgatgcc gacaagccac tccgatcgcg ctcgctctcc gcaaccgtct
     3061 cgcccagcgt gccgcgcagc gccgacgcca accgcgcagt caccggcgca accgcatcga
     3121 gcgccggcgc ttccacggtt tcttcgaaca ccagcgctcc agtggaatcg gtgaccgtca
     3181 gtccgatctg cagcttggcg ccgcgctcga tcgccgtcgg atgaaccgtc aagacgcgcc
     3241 cgccatcgcg ctctgcgatt cgcgccgcaa gcttgtcgtc gatcgcgaca tcattgccga
     3301 acgccgacgc cacccggcgc aacgacgcgc ccgtgatcgg gtcgagcacc gtcgaccgcc
     3361 gcagcgacga ggtcacgatg atctctaccg ttccgtcgag cgacgtctct gacgtcgtgt
     3421 tcgtgagcgg caagacgagc gtcgtcatcg ggcttgtcgt ggcgacgggc ccggcacccg
     3481 cctccggccc tcgtgccgac gcgatgatga gagcaggccg ctcgttcggc gacgcgcctt
     3541 tcttcgaccc gcccgaaatc acgagaactg ccacgacacc gcccgccgcg cacaacgcac
     3601 cagccgcgat cgccgggatc aaccacgtcg gacgccccgc cgcgacacgc cgcacaggct
     3661 ctgtcgtcac gcctccggtc tcgaccgcgc cgagcacctc cttcacggtg cgatagcgac
     3721 cggacggatt gagcgccaag cacttcgcag cgattccctc gaggtacgca ggcgcgtcgg
     3781 gccgcacgtc acgcagaagg cgcggcttct tgtgcaggcg catctccatc acggcatgcg
     3841 gcgtgtccgc cttgaacggc gtctcgcccg tgacgagctg atacagcatg atgccgagcg
     3901 agtagatgtc gctgcgctcg tccgtcgggt cgcccttcac ctgctcgggc gacatatacg
     3961 ccggcgatcc gagcaccgca ccgctcgccg tcagccccga ctccgtaatc gagcgcgcca
     4021 atccgaagtc tgcgacgcgc acgccgccat ccttgtcgac gagcacgttc tgcggcttca
     4081 gatcgcgatg caccacgccc tgcgcgtgcg ccgctaccag cccctcgcag acctgacgaa
     4141 agatccgcac cgcgcgatcg atgggcagct gcttctcgcg ccgcatgagc gccgccaagt
     4201 cttcgccgtc gacaaactcc atcgacaaga agcgcaagcc atcatgctcg gagatgtcgt
     4261 agacgcgcag cacatacggc gaggtgacct tacgcgcgag cgcgatctcg cgacgaaacc
     4321 gcgtgagcgc ctcttcgtgg tcagcatcgc cgcgcacgat cttgagcgcg acctcgccct
     4381 tcaactccgt atcgtaggcg cgaaagacct cacccatgcc gcccttgccg acgatcccga
     4441 tgacgcgata ccgcggtccg aaatcgggtt gcacgcgcaa cgacggctga gagacgcggc
     4501 ctggtttgtc ctgtctcgtc gcgtcgtccg actgatccgc caccatcgac gatcgtatca
     4561 gaggcgtgca ctccccgatc ggcggattgc attcggattc gagatcgcgc gaccttcccc
     4621 gaacatgcgc atcgcgatca tcggaagcgg aaatattggt ggaggcctcg gccgagcgtg
     4681 gaagcgcaaa ggccacgacg taacgttcgg agcccgcaac cccgaggacg ccgagctgct
     4741 cgcgctctgc aacgagacgg gcgcacacgc ggcgtcggtg agcgacagcg tacgcaacgc
     4801 cgacgtcatc gcgctggccg ttccgtatgc ggcgctcgac gaggtgctcg ccgcgacggg
     4861 cgagctcgac ggcaaggtcg tcatcgattg cacgaacgcc gtcgagcgcg gcgccggggg
     4921 catgcagctg aagtacggcc acacgacgtc gtcagcggag caactgcaag cgcgcattcc
     4981 gaaggcgcat gtcgtgcgct cgttcaatgc acagggcgca gagaatcttg cgaacccgtc
     5041 gtacggcggt gtgcccgcat cgaacttcta ctgcggcgat cacgctgatg cgaagcgcgt
     5101 ggtcgcgcag ctcgttagcg atgtcggatt cgaagcggtg gacgcgggtc cactcgcgag
     5161 cgcgcgctac ctcgagccgc tgatgctgct gtggatcaac gtgtcgagga ccgtcgggtc
     5221 gcgcgacatc gcgttcaagc tgctgcgtcg ttagccacgc cgcgttcgcg cgtcggacag
     5281 tccgcaagcg atgcgcgcct tcgtgcggcg acacgaccgg cgctcatcgc tcgccgcagt
     5341 ggcgcaacgc ctctcggatc agcacgccga gctcgacgcc cgtgcccacg tgggcacaag
     5401 cctcgtcgac cgcgcgcgcc gcaagcggag ctttgaagcc gagctgccga agcgcggcct
     5461 gcgccagcgt ggcgggatca tccctttcga gatcaggcgc gttcatcgcc ctcttcacgc
     5521 tcgctgccgc gacgtgcttg cgcggtgcgt gcccttcgcg tcccgccagc ggcactccgc
     5581 cgcgttcgaa ggcgagcttg tcgggcgctt cgcccgtgat cttcagaagc ccatcgtgca
     5641 gcaagcgatg gtgcccgtcg cacagcacga tcaaattcga gagctcgtgc gtgccgccgt
     5701 gcgaccagaa cacgatgtga tgaatcgcca agtacctcga cgagcggcag ccaggtacct
     5761 cgcaccgatg ctgcgcgcgc tcgagcacga gacgctcgat gcgagctgga atggtgcgct
     5821 tcggtcgcat ccccttctcg tcgtcgcaga taaccgcgtt gcactgcgcg cggtcgacga
     5881 cggagcgatc gacttcgacg cgcacgcctg cgccgtcctg ccaaccgcgc ccacaatcgc
     5941 gacaggtcgt cagctgaatc atgtaccggg cgaccacgct tcgaagcggc gccttcttat
     6001 cggccacggt cgtatcgaca ttgccgttcg cggctgcagc gcccggcgcc ggcaacgtcc
     6061 gcgccgccgc cgttgtctcc gcctccgccg ccgccgcctc cacgcccgac gcgggcgccc
     6121 cggctgccgg accgcccgcg tgcagcgact cttcgcgacg acgatgtccc gcctcgccga
     6181 gaatgcgacg gccgatcgcg agcaccaagt cgcgatcgtc gagcttgcgg tcgctctcga
     6241 tctcgatcga ctgctgcatc tgccgccaaa ccacgaagct ctcaccgtcg agctcgagga
     6301 ccacgcgatg cttcacgcgc gccggatccg cagggtcgcc cggcaggtcg cccttcttgc
     6361 gacccgcgac catctgctcg acatcgcgaa gccgccgccc acgcgcctcg tcgagccaac
     6421 gctcgatcgt gtcagacgtc gcgacgcgcg tcagctcgcg cgcgaccgac caactcgtct
     6481 cgccgcagcg caacgcgtcc tcgagctgcg gcatgtcacc cagctcgcgc gcaacgcgca
     6541 accgctcctt cgcggttcgc ggcgcatagc caaagacctc ttcgaggtac tcgaaggcgt
     6601 gcacgtaccc gagcttcggc catacacgct gctgctcggc gatgcgaagc caatgcgctt
     6661 cgtccgcgtc gagagctgca tgcgcaactg caatccgtcg cagcgcgcga tcgacctgcg
     6721 tgtgttgcgc gacaacctcg cgctcgacct tcaccattcc atcgcgtgtc atgaacgttg
     6781 cgtaccacgc gatatttcgg ttgtgccgtt tgcgtcgtgc gcgcgcaacg cgcgttttct
     6841 cgtcgagaaa tcggtcgcga cgatcgtgtg cgtgagatcg cgtgcagtgc gcgcaggcga
     6901 ttgatcggaa g
//
LOCUS       DATLBZ010000299         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_335129, whole genome shotgun sequence.
ACCESSION   DATLBZ010000299 DATLBZ010000000
VERSION     DATLBZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335129"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2215)
                     /locus_tag="VMZ53_15400"
     CDS             complement(<1..>2215)
                     /locus_tag="VMZ53_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29890.1"
                     /translation="TQSIPLIVNDAQTVKSFTPMHAPQVVGEAIAILAGRKCHDATGE
                     LVRRQRRQLFEDRFAEHCSRPEDKAPLPAATVVDSIGTYARGQVGIGETTSFMRVFIE
                     NRLFRELHVPFALPIVANLDLGAEQCSETFEEIPTPVASKMLNDALRAVPRLLVAAGS
                     AQPQQLAEVGATRRLLAWASADLPDDARQQLCAIEAFKTVQGTRVSLETAWSPQRVLR
                     RAAWQDEWLGPAPGQPASSHDEPILAVATEDAELRTVLEKLSGKPVIDVTDEVVRLQA
                     NRRMARGLIPTPKVNGVPPELKRPLSELGEQGQKLGHGELALIDGDQSLINLHSQGRF
                     VRSIPFSVQPPVALAIEDHEVAKPEQLRDIQKLVDEFLGEHPLTHTARALAIELVSQV
                     LATPARSMLTPRMLRNLARAVWARRLSPKLLGDLAAFETLDHQWVTWETLTDQVAKLG
                     DVWAVSEWTDERPLDERRIVLLATREDIDSTKQQNVKLVEAREELRLDGIARRNRAMP
                     PLTSLALPTRLGLLAEAELEGDGTHGPRGTVAILIPSVRGSRGVHVHRGMQPLGTMPD
                     PCPWPTLAVIDDARLTPDRTWSAAKPDQIWQAIGKTIRAASEALLATFGEAPSHAPVE
                     MRIDAVASGDIKELRKSHKSQMRGVVWLEPHPLSEANLQLTYEVGVTTVRNLPVSGQV
                     LLHSDGALERQAALEQLGAYAHGRLVRKLVAESDKHDDDMVQAHVAHALALDTVKP"
BASE COUNT          334 a          744 c          744 g          393 t
ORIGIN      
        1 cggcttcacg gtgtcgagcg cgagcgcatg cgcgacgtga gcctgcacca tgtcgtcgtc
       61 gtgcttatcg gactccgcga cgagcttgcg cacgagccgg ccgtgcgcgt acgcaccgag
      121 ctgttccagc gccgcttgcc gctcgagcgc gccatcggag tgcagcagca cctggccgct
      181 gaccgggagg ttgcgtaccg tcgtgacgcc gacttcgtac gtcagctgca gattcgcctc
      241 cgagagcggg tgcggctcga gccagacgac accgcgcatc tgcgacttgt ggctcttgcg
      301 aagctctttg atgtcaccgc ttgcgaccgc atcgatgcgc atctcgacgg gcgcatgcga
      361 aggcgcctcg ccaaacgtcg cgagcaacgc ttcgctcgcg gctcgaatcg tcttgccgat
      421 cgcttgccag atttgatccg gcttcgccgc ggaccacgtt cgatcgggtg tcaggcgtgc
      481 gtcgtcgatg actgcgagcg tcggccacgg acacggatcg ggcatcgttc cgagcggctg
      541 catcccgcga tgcacgtgca caccgcggct acctcgaacc gatgggatca ggatggcgac
      601 cgtcccgcgc gggccgtgcg tgccgtctcc ttcgagctct gcttcggcga ggaggcccag
      661 cctcgtcggc agtgcgagcg acgtgagcgg cggcatcgcg cgattgcggc gagcaattcc
      721 gtcgaggcgc agctcctcgc gtgcttcgac cagcttcacg ttctgctgct tcgtgctgtc
      781 gatgtcttct ctcgttgcga gcagcacgat gcggcgctcg tcgagcggtc gctcgtcggt
      841 ccactcgctc accgcccaga catcgccgag cttcgcaact tgatcggtca acgtctccca
      901 cgtcacccac tgatgatcga gcgtctcgaa tgcggcgagg tcaccgagaa gcttcggcga
      961 gagtcgacgc gcccataccg cacgcgcgag gttgcgcaac atgcgcggcg tcagcatcga
     1021 gcgcgccggc gtcgcgagca cttgcgagac gagctcgatc gcgagagccc gcgcggtgtg
     1081 cgtcagcgga tgctcgccga ggaattcgtc gacgagcttc tggatgtcgc gcagctgctc
     1141 gggcttggcg acctcgtgat cttcgatcgc gagcgcgact ggcggctgca cgctgaacgg
     1201 aatgctccgc acgaaccggc cctgcgagtg gagattgatc agcgactgat cgccgtcgat
     1261 cagcgccagc tcgccatggc cgagcttctg gccttgctcg ccgagctccg agagcgggcg
     1321 cttcagctcg ggcggcactc cgttgacctt gggcgtcggg atgaggccgc gcgccatgcg
     1381 gcgatttgct tggagccgca cgacctcgtc ggtcacgtcg atgacgggct tgccagagag
     1441 cttctcgagc acggtgcgca gctcggcgtc ctcggtagcg accgcgagga tcggctcgtc
     1501 atgcgacgaa gcgggctggc ctggagcggg accgagccac tcgtcttgcc acgccgctcg
     1561 acgcagcacg cgctgcggac tccacgccgt ctcgagcgac acgcgcgtgc cctgcaccgt
     1621 cttgaatgcc tcgatcgcgc acagctgttg gcgtgcatcg tcgggtagat ccgcgctcgc
     1681 ccacgcgagc aaacgccgtg tcgcgcccac ctcggcgagc tgctgcggct gcgcgctgcc
     1741 ggcggcgacg agcaagcgag ggactgcgcg gagcgcgtcg ttcagcatct tcgatgcgac
     1801 cggcgtcgga atctcttcga acgtctcgct gcattgctcc gcaccgagat cgaggttcgc
     1861 gacgatcggc agcgcgaacg gcacgtgcag ctcgcggaac aggcgattct cgatgaagac
     1921 gcgcatgaag ctcgtcgtct ctccgatacc gacctgaccg cgcgcatacg tgccgatcga
     1981 atcgacgacg gtggcagcgg ggagtggcgc cttgtcctcg gggcgagagc agtgctcggc
     2041 gaagcggtcc tcgaacagct gccgccgctg tcgccgcacg agctcgccgg tggcatcgtg
     2101 acacttgcgt cccgcgagga tcgcgatcgc ttcaccgacg acctgcggcg catgcatggg
     2161 cgtgaagctc ttcaccgttt gcgcgtcgtt gacgatgagc gggatcgatt gcgtc
//
LOCUS       DATLBZ010000300         5847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2572927, whole genome shotgun sequence.
ACCESSION   DATLBZ010000300 DATLBZ010000000
VERSION     DATLBZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572927"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VMZ53_15405"
     CDS             complement(<1..377)
                     /locus_tag="VMZ53_15405"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HUS29891.1"
                     /translation="MSREFDIVMFGATGFTGQLVAEYIKGKKPARWAIAGRNKAKLEA
                     LGLDAPIIVVDAHDRAGLDAVAKRTKVVCTTVGPYAKYGSELVAACANAGTHYCDLTG
                     EVHWMRAMIDANHDRAVQTGARIV"
     gene            432..1001
                     /locus_tag="VMZ53_15410"
     CDS             432..1001
                     /locus_tag="VMZ53_15410"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HUS29892.1"
                     /translation="MSPLVESLVGKLKLGESLRPPDDGRRRAAVAALLHDEGPSPRVL
                     LMKRTEREGDPWSGHISLPGGGYQRSDEDLLATAIRETHEELGIDLKGARLLGNMPHL
                     HPFTSGPMGVEVTPFVFVTTAAVEPQPGPEAESAFWLPLDVAASGQIDGTYTYPGTDK
                     TFPCWTFERYTIWGLTWRILGDLLALAKA"
     gene            complement(991..2151)
                     /locus_tag="VMZ53_15415"
     CDS             complement(991..2151)
                     /locus_tag="VMZ53_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUS29893.1"
                     /translation="MAQNVVLGRVLVVDDDDAIRRVCGRVLSAEGWEVVSAQNGRAAI
                     EMLEASKLPFDCVVSDVNMPEVDGYELVQAVRKRDDDIPVLLMTGDPSLDGAVRAIDY
                     GAVSYISKPFSSEALASAVARAARRHGVTRMRRRADSYMKAIGGNDATDSIEARFSRA
                     LAQSWMAFQPIVDISAKRVFAYEGLLRTDEESLRRTDIFIATAERLDKVHALGRTVRA
                     AVARAAADAPEGVLLFVNVHGLELTDEDLFAPSSPLAKLANRVVLEITERTGLDPAAG
                     PTRVAMLRKLGYRIAVDDLGAGYAALGALATLEPEIVKLDMSLVRDLDQHPTKRRVVA
                     AIANLCRELGSRVVAEGVETEAEKTAIIDSGIDLIQGYLLAKPAKGFVPVTL"
     gene            complement(2164..3291)
                     /locus_tag="VMZ53_15420"
     CDS             complement(2164..3291)
                     /locus_tag="VMZ53_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS29894.1"
                     /translation="MSSEIGVVSEASVLPGAVRLLVVDDDVAICRQLASGLANAGFQV
                     VTAHDAEGALAQAAQTPPDVAIVDLGMPGVSGFDVIRQLKQMQGGAVHVIVLTGQDDE
                     ASRAEAFDAGTDDYVLKPIGVSEIRRRVSAAIRNQRAFVQVRLEKEAAERRLVYGQEA
                     SALLAHDLNNGLSVSLSNLSYLLDVVTGDQEVNEALAATLRSVRRMSGLVANFVDIAR
                     FEDAAVKPMVSRAKVRGLLQSVIEVNASSAKKGAALEIDCTPELEARFDIGLVERVLH
                     NLFGNAMRYCNAGGRVILAAKRWHETESASVEIAVTNSGPQIPENIRGNLFGKYVQGK
                     GGKRGMGLYFCRLVAEAHGGNISYEPRDDGPSFVLRLPGHA"
     gene            3318..4595
                     /locus_tag="VMZ53_15425"
     CDS             3318..4595
                     /locus_tag="VMZ53_15425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29895.1"
                     /translation="MTPTLRVVDPSRLADLTLAAALRSSSDAVYLEPGKTDDSFLITF
                     QRAQQNVAAISVEALSGAATIARLALIAGLDLASPTAASAVIKVRSGDRDADVVVTVR
                     PGAGSLRADLMVMAKAPRGMPAPRTTITAAAQVTGEPGDVVGNYKILERLGEGGMGTV
                     FRVEHAVLGRHYALKVLRSQVVEKAATAAQRFVREARAAARVRHPNIVDVFDFGYFPD
                     GRPFFVMELCEGQSLLEYVRRQGPLPPSDAIAIARQIALALAQVHDHGVIHSDVTPSN
                     VLVVGTRPLEVKLLDFGLAAIADELFEDENSDFVLGTPAYISPEQLHGLPATDRSDQY
                     GLGCVLYEMLVGHPPYQHENVRELCMMHIQAPIPVAESMYGPLPAKLTNAIATCLQKS
                     PQARFPGLRALIAVLDDIDRGNDRRGWQKWLNT"
     gene            4595..5167
                     /locus_tag="VMZ53_15430"
     CDS             4595..5167
                     /locus_tag="VMZ53_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS29896.1"
                     /translation="MAKWLIVCVDDDEAILSAVARTLRREPSFEVRPTMDVQQVLEWI
                     HREDVAVLVSDYEMPEITGAQLAGIVKNIRPETVRILLTGRRTLDTAIDGINQGEIFR
                     FIGKPFEDKQLRQAVLDAAKRHDELMALSGDRQRRQRREQLLTALEQEYPGITKVDRR
                     TNDVVVVTDDPWSTAAELGIAELDPSLESS"
     gene            5164..5799
                     /locus_tag="VMZ53_15435"
     CDS             5164..5799
                     /locus_tag="VMZ53_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29897.1"
                     /translation="MIEAEPHLDETGKPCTRHAALSQDALLAYAAVGSRVSGFHHDAA
                     SKMQSLMMAIDEITELGNDDVRAAAATASTALRDLHQILTVNRALAKTPQRKSTPVRD
                     LITRAAERHGVKVRGELPATTVHVALASVAHALSIALDIAAGPLQAARTVDVTCTPGT
                     HLAVAMTATGELGAPPNANELLALASFLLRREDATLTCKPNGFVVELPLAQ"
BASE COUNT         1043 a         1979 c         1826 g          999 t
ORIGIN      
        1 acaatgcgag cgccggtctg cactgcgcga tcgtggttcg catcgatcat cgcgcgcatc
       61 cagtgcacct cgccggtgag atcgcagtag tgcgtgccgg cgttcgcgca cgccgcgacg
      121 agctcgctgc cgtacttcgc atacggaccg acggtggtgc acacgacctt cgtgcgcttc
      181 gcgacagcgt cgaggcccgc gcgatcgtgc gcatcgacga cgatgatcgg tgcgtcgagc
      241 ccgagcgctt cgagcttggc cttgtttcgg cccgcaatcg cccaccgcgc cggcttcttg
      301 cccttgatgt actcggcgac gagctggccg gtaaacccag tcgcgccgaa catcacgatg
      361 tcgaattcgc ggctcataaa acgcgcttct ccatgcctgc cgcatcgtat gcgatccgct
      421 atcctgaaac cgtgtcaccg ctggtcgaga gcctcgtcgg caagctgaag ctgggcgagt
      481 cgctgcggcc tccggatgac gggcgccggc gcgccgcggt cgcagcgctt ctgcacgacg
      541 agggtccgtc accgcgagtc ctcctcatga agaggacaga gcgcgagggc gatccgtggt
      601 ccggtcacat ctcgttgccg ggcggcggct atcagcgcag cgacgaagac ctcctcgcga
      661 ccgcgatccg cgagacacac gaagagctcg gcatcgacct gaagggcgcg cgtctgctcg
      721 gcaacatgcc gcacctgcat ccattcacgt cgggaccgat gggcgtcgag gtcacgccgt
      781 tcgtcttcgt gacgaccgca gccgtcgagc cacagccggg ccccgaggcg gagtctgcgt
      841 tttggctgcc gctcgatgtc gccgcatcgg gacagatcga cggtacgtac acgtatccgg
      901 gaacggacaa gacgttcccg tgctggacgt tcgagcgcta cacgatctgg ggactgacct
      961 ggcgcatcct cggcgaccta ctggccctcg ctaaagcgtg acgggcacga agcccttcgc
     1021 cggcttcgcg agcaagtagc cctgaatcag atcgattccc gaatcgatga tcgctgtctt
     1081 ctcggcttct gtctcgacgc cttcggcgac gacgcgactg ccgagctcgc gacatagatt
     1141 cgcgatcgct gcgaccacgc gacgcttcgt cggatgttga tcgagatcgc gaacgagcga
     1201 catatcgagc ttgacgatct cgggctcgag cgtcgcgagc gcgccgagtg ctgcgtagcc
     1261 tgcgccgaga tcgtcgacgg cgatgcgata gccgagcttg cgtaacatgg cgacgcgcgt
     1321 ggggccggct gcggggtcga gaccggtgcg ctcggtgatc tcgaggacga cgcgattcgc
     1381 tagcttcgcg agtgggctcg acggcgcgaa cagatcctcg tcggtcagct cgagcccgtg
     1441 cacgttgacg aacaggagga caccttccgg cgcatccgcc gcagcacgtg ccaccgccgc
     1501 tcgaaccgtg cgaccgagtg cgtgcacctt gtcgagacgc tccgcggtcg cgataaaaat
     1561 gtcggtgcgg cgcagcgatt cctcgtcggt gcgcagcaag ccctcgtagg cgaatacgcg
     1621 cttggcggag atgtcgacga ttggctggaa cgccatccat gactgcgcga gcgcgcgaga
     1681 gaaccgcgcc tcgatgctgt cggtcgcatc gttcccgccg atcgctttca tgtacgagtc
     1741 cgcgcgacgc cgcatgcgcg tgactccgtg ccggcgcgct gcacgtgcga ccgctgacgc
     1801 gagtgcctcg gacgagaacg gcttcgagat gtacgacaca gcgccgtaat cgatcgcacg
     1861 cacggcgcca tcgagtgacg gatcgccggt catcagcaga acgggaatgt cgtcgtcgcg
     1921 cttgcgcacc gcttgcacga gctcgtagcc gtcgacctcc ggcatgttca cgtccgacac
     1981 gacgcagtcg aatggcagct tcgatgcttc gagcatttcg atcgcggcgc gtccgttctg
     2041 cgcagagacg acctcccagc cctcggcgct gagcacgcgg ccacagacgc gccggatggc
     2101 atcgtcatca tcgacgacga gcacccgacc taatacgacg ttctgcgcca caacgagctc
     2161 ctattatgcg tgtcctggaa gccgcaggac gaagctcggg ccgtcgtcac gcggttcgta
     2221 ggagatgttg ccgccgtgag cttcagctac gagacggcag aagtagaggc ccatcccgcg
     2281 cttgccgcct ttgccctgca cgtacttgcc gaacaggttg ccacggatgt tctccggaat
     2341 ctgcgggccg ctgttcgtca cggcgatctc gacgctcgcg ctctccgtct cgtgccaccg
     2401 ctttgcagcg aggatgacgc gaccacctgc gttgcagtag cgcatcgcat tgccgaacaa
     2461 gttgtggagt acgcgctcga cgagaccgat gtcgaatcgc gcttcgagct cgggcgtgca
     2521 gtcgatctcg agtgccgcac ccttcttcgc cgacgacgca ttgacctcga tgacggactg
     2581 cagcagcccg cgcaccttcg cacgcgacac catcggcttg accgctgcat cttcgaatcg
     2641 cgcgatgtcg acgaagtttg cgacgagacc ggacatgcgc cgaacggacc ggagcgtcgc
     2701 ggcaagcgct tcattgacct cttggtctcc cgtcacgaca tcgagcaggt acgagagatt
     2761 gctgagggag acggacagac cgttgttgag atcgtgcgcg aggagtgcgc tcgcttcctg
     2821 gccgtagacg aggcggcgct cggcagcctc tttctcgaga cgaacttgca cgaacgcgcg
     2881 ttggttgcga attgcggcgc tgacccgccg acggatctcc gagacgccga tcggcttgag
     2941 cacgtagtca tcggtaccgg catcgaatgc ttcggcgcgg ctcgcttcgt cgtcttggcc
     3001 ggtcaacacg atcacgtgca ccgcgccgcc ctgcatttgc ttgagctggc ggatcacatc
     3061 gaagccactg acgcctggca tgccgagatc gacgatcgcg acatccggag gcgtctgcgc
     3121 cgcttgtgcg agtgcgcctt cggcatcgtg cgcggtgacg acttggaatc ccgcgttcgc
     3181 taatccgctc gcgagctggc gacagatcgc aacgtcatca tcgaccacga ggagtcgaac
     3241 ggcgccgggg agtaccgacg cctccgagac caccccgatc tcagatgaca tgcgtgtgtt
     3301 agtatcatcg gcgcttcatg acgccaacac tgcgcgtcgt cgacccatcg cgcttggcgg
     3361 acctcacgct ggcagctgca ctgcgatcct cgtcggacgc ggtgtatctg gagcccggga
     3421 aaacggacga cagcttcctg attacattcc aacgggcgca acaaaatgtt gcagcgattt
     3481 cggtcgaagc actgagtggc gcggccacga tcgcccgtct cgcactcatc gcgggtctcg
     3541 atctcgcgag cccgacggcc gccagcgcag tgatcaaagt tcgctcgggc gatcgcgacg
     3601 cagatgttgt cgtcacggtt cggccaggtg caggctcgct gcgcgccgat ctgatggtca
     3661 tggccaaggc gccgcgcggc atgccggctc ctcgcaccac gattacggcg gccgcgcagg
     3721 tcacgggtga gccgggcgac gtcgttggca actacaagat tctcgagcgg ctcggcgaag
     3781 gtggcatggg caccgtgttc cgcgtcgagc atgcggtgct cggccgccac tatgcgctga
     3841 aggtgttgcg ctcgcaggtc gtcgagaaag cggcaacggc tgcccaacgg ttcgttcgcg
     3901 aggctcgcgc ggccgcacgc gttcgccatc ccaacatcgt cgacgtcttc gacttcggct
     3961 acttccccga cggacgcccg ttcttcgtga tggagctctg cgagggccag agcctgctcg
     4021 aatacgtgcg ccggcaaggc ccgctcccac cgagcgacgc gatcgccatc gctcgccaga
     4081 tcgcactcgc actcgcccag gttcacgacc atggcgtgat ccactccgac gtgacgccgt
     4141 cgaacgtgct ggtcgtcggc acgcgaccgc tcgaagtgaa gctgctcgac ttcggtctcg
     4201 ccgcgattgc cgacgagctg ttcgaagacg agaacagcga cttcgtgctc ggcacgcccg
     4261 cgtacatctc tcccgagcag ttgcacggtc tgccagcgac cgaccgcagc gatcaatacg
     4321 gactaggctg cgtgctgtac gagatgctcg tcgggcatcc gccgtatcaa cacgagaacg
     4381 tacgcgagct gtgcatgatg cacatccaag cgccgattcc cgtcgccgag agcatgtacg
     4441 gcccgctgcc ggcaaagctg acgaacgcga tcgcgacgtg cctgcagaag tcgccgcaag
     4501 cccgcttccc cggcctgcgt gcactgatcg ccgtgctcga tgacatcgac aggggcaacg
     4561 accgccgcgg ctggcagaaa tggttgaaca cgtaatggcg aagtggctca tcgtgtgcgt
     4621 cgatgatgac gaagcgatcc tgtccgccgt cgcacgcacg ctgcgtcgcg agccgtcatt
     4681 cgaagtgcgg ccgacgatgg atgtccaaca agtgctcgag tggatccacc gcgaagacgt
     4741 cgccgtgctc gtctccgatt acgaaatgcc ggagatcacg ggcgcgcagc tcgccggcat
     4801 cgtgaaaaac atccggcccg aaacggtccg catccttctc acgggccgcc gcacgctcga
     4861 caccgcgatc gacggcatca accaaggcga aatctttcgc ttcatcggca agccgttcga
     4921 agacaagcag cttcgccaag ccgtgctcga cgctgcgaaa cgtcacgacg agctcatggc
     4981 gctgtccggc gatcgccagc gccggcaacg ccgcgaacag ctgctgaccg ccctcgagca
     5041 ggaatatccc ggcatcacga aggtcgatcg ccgcacgaat gacgtcgtcg tcgtcaccga
     5101 tgatccatgg agcacagccg ccgagctggg aatcgccgag ctcgatccgt cgctggagtc
     5161 ctcgtgatcg aagccgagcc gcatctcgac gagacgggca agccgtgcac gcgccacgcg
     5221 gcgctctcgc aagacgcgtt gctcgcatac gccgccgtcg ggtcacgagt ctcgggcttt
     5281 caccacgacg ccgcgtcgaa gatgcagagc ctgatgatgg cgatcgacga gatcacggag
     5341 ctcggcaacg acgacgtgcg cgcagcagcc gccactgcct ccacggcgct ccgcgacctg
     5401 caccagatcc tcacggtcaa ccgcgcgctc gcgaagacac cgcagcgcaa gtcgacgccc
     5461 gtccgcgacc tcatcacgcg cgccgccgaa cgccacggcg tgaaagtccg cggcgaactg
     5521 ccagcaacca ccgtgcacgt cgcgttggcc tccgtcgcgc acgcactgtc gatcgcgctc
     5581 gatatcgccg cgggtccact acaagccgcc cgcacggtcg atgtcacgtg cacgccaggc
     5641 acccatcttg cggtcgccat gacagccaca ggcgagctcg gcgcgccgcc gaatgcgaat
     5701 gagctcctcg cgctcgcgag cttcctgctc cgccgtgaag acgcgacgct cacgtgcaag
     5761 ccaaacggct tcgtcgtcga gctaccgctc gcgcaataac gcgcgctcgc cgctggtcaa
     5821 tccatacgcg tcgttcacga gctccgc
//
LOCUS       DATLBZ010000301         5325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4049565, whole genome shotgun sequence.
ACCESSION   DATLBZ010000301 DATLBZ010000000
VERSION     DATLBZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4049565"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..802)
                     /locus_tag="VMZ53_15440"
     CDS             complement(191..802)
                     /locus_tag="VMZ53_15440"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HUS29898.1"
                     /translation="MDFLVFGASGRTGGAFVRQAVAANHAVGAFVRDASSLPAGVDAH
                     VGDVLDAAAVSAAIRGTPTIIIALGGVDALTRGAANVIDAATSAGVRRVLGVVGAGVL
                     QADAARQRHEMPDYPPRFRPIGAAHAAVLTALRASPLEWTLACTPNIVDGPATGTFAE
                     RADYLPDGTGSISTGDIAAFLLREAIASRYPRSRVGLNGSPRA"
     gene            complement(808..2511)
                     /gene="ffh"
                     /locus_tag="VMZ53_15445"
     CDS             complement(808..2511)
                     /gene="ffh"
                     /locus_tag="VMZ53_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HUS29899.1"
                     /translation="MLETLAKGFRAARQRLTGVAELNDDIIDEALRDVRLSLLEGDVE
                     FRVVKRFLERVKEQARGKQVELRAKSAEYGVKSITPEQAFIAICQDELIKMMGPVDTE
                     LNWAKGKGPTGIMMCGLQGSGKTTTVGKLARWLEKTHKKKPMLVAADIYRPAAVEQLK
                     TLGRQLNVPVYSAEGKDPVTICKEANEAAAREGCDVVIFDTAGRLAIDEPLMQELDDI
                     NKSVTPANIFLVLDAMTGQDAVNVADTFNKRLNLDGVIMTKLDGDARGGAALSVKEIT
                     GKPIKFLGMGESLDKLEEFRPEGLASRILGMGDIVGLVKDFEQVVDAEKAEEDALRML
                     KGKFDMQDFLEQIKLIQKMGSLKDLFEKLPFFGGGLPEGVNLDDRELVKIEAMISSMT
                     REERTNPQVFVTTSWEDFATTAGKQAKRRRADFDTGRVKRISKGSGRGENEVKELLQK
                     FAQMRQMMVQLGASTGLMGKMPGFKQFSQMKKLANMDINALMQGGGLPPELAGALPGG
                     LPNMPGMPGLPTGNIPGLPKGYTPPGTKSLVGGQQRSNDKQKQRDKRKAEKAARKKNR
                     R"
     gene            complement(2554..3000)
                     /locus_tag="VMZ53_15450"
     CDS             complement(2554..3000)
                     /locus_tag="VMZ53_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29900.1"
                     /translation="MRMISLRRLLSKLTVLALSASPAAAAAGDAAPSAPSVDDIDLDV
                     ALDEDIPIDREHIDPQDAATVLGRSLAKALSEMREIEAVHLSTAWALSADPTRRAGIA
                     HALEWPFRLMADRLIIDHLSQDPDPLVRSACARASWIRGIGSEWRV"
     gene            3104..4360
                     /locus_tag="VMZ53_15455"
     CDS             3104..4360
                     /locus_tag="VMZ53_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29901.1"
                     /translation="MKSCRQIALLVALTACGSSSSKPTTPQPDVPTPTTTSTQTVATT
                     PASTGPIEPATAPAQGNRCDMASVGELSIDGLVDDWKASHVVGRIGSPKDGAVELHCV
                     WDGQSLALMMDIKDDRLVRVKGGHEDHVSISLAAGGKPVLVDIFPGNSMAKPKITKPA
                     KVQAADSLQQGGFSVEVEIPASAIPDFSPSTPALQLEATFHDSDAATGGDDTPLRLTQ
                     PIELGDRKDLLDDFLSTVRLKRTDIKLDQLVDLDPDHPGKERLVYGGTVIGILTDVYG
                     YVSMPASAAADVKKVELLPLGPKGSQIISALVRQTGNGGSRDLLMLWTVWSGQLQPLV
                     TIEVKKELAGNVLESTWKITKGKKGPELVVEPKPAVGFSADDYAEVPAEDADAILLPW
                     DDKKAGIAYSVTGKEITRRDLPKKKR"
     gene            complement(4357..4914)
                     /locus_tag="VMZ53_15460"
     CDS             complement(4357..4914)
                     /locus_tag="VMZ53_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS29902.1"
                     /translation="MRWAVCAALVSTACGRLAFDDLSGTRDATDGVASDAVQGDASGD
                     AVLAACAGIQCTGAPKQMMCDGRCITLCPDSVSQDTADTRCIAWGGQLATVHDATDDA
                     CVSQLTNPPAWIGYMQQAGATTVAGNWSWLDGVASPYVNWGTGEPQDGQGTEDGEEQC
                     AVMFADGLWYDTSCSAATFPFVCTR"
     gene            complement(4941..>5325)
                     /locus_tag="VMZ53_15465"
     CDS             complement(4941..>5325)
                     /locus_tag="VMZ53_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29903.1"
                     /translation="WRVDLLNLDGKGKEAEKLARDLQVTIEELGAGELDQWAFHRKLA
                     DSFRVQKRYAEALVEGTKALEIANKQKLRPDLIALDEILVAQIESKLGKREQAIKRAK
                     AALELFKTYKGQPRAEREAQKILAH"
BASE COUNT          919 a         1788 c         1680 g          938 t
ORIGIN      
        1 tgccagatca gcggcatcac cgcgaagcga tacgtgatca agctgaacga cacgccccac
       61 agcagcgatg caacgaacag cgacgcgcga gccgacacga gcggaccata tagcggcggc
      121 gtcgcgccgt cgatgacctt cgcgccgtcg atgaaccttc gcgccgtcga tgaccttcgc
      181 gccgttcgcg ctatgcgcgg ggcgagccgt tgaggcccac gcgcgagcgc gggtagcgcg
      241 acgcgatcgc ctcgcggagc aagaacgcgg cgatgtcgcc cgtcgagatc gagccggtgc
      301 cgtcgggaag gtagtcggcg cgctccgcga aggtgccggt cgctggaccg tcgacgatgt
      361 ttggcgtgca cgcgagcgtc cactcgagcg gcgaggcgcg cagcgcggtg aggactgcag
      421 cgtgcgcggc gccgatggga cgaaaccgcg gcgggtaatc gggcatctcg tgacgctggc
      481 gcgccgcatc ggcttgcagg acccctgcac cgacgacgcc gagcacgcga cgcacgcccg
      541 ctgaggtcgc ggcgtcgatc acgttggccg cgccacgcgt cagcgcgtcg acgccaccga
      601 gcgcgatgat gatcgtcggc gtgccccgaa tcgccgcgct cacggccgct gcatcgagga
      661 catcgccgac atgcgcgtcg acgccagccg gaagcgacga cgcatcgcgc acgaacgccc
      721 ctaccgcgtg attcgccgca acggcctggc ggacgaacgc gccgcccgtg cggcccgatg
      781 caccgaagac caggaagtcc acgcgaccta gcggcggttc ttcttgcgcg cggccttctc
      841 ggctttgcgc ttgtcgcgtt gcttctgttt atcgttcgag cgctgctggc caccgacgag
      901 gctcttcgtg cctggaggtg tgtagccctt cggtagaccg gggatgttgc cggtcggcag
      961 ccccggcatg cctggcatgt tcggcagacc gccgggcagc gcgcccgcga gctcgggcgg
     1021 aagaccgccg ccttgcatga gcgcgttgat gtccatgttc gcgagcttct tcatctgcga
     1081 gaactgcttg aagccgggca tcttgcccat gaggcccgtc gacgcgccga gctgcaccat
     1141 catctgacgc atctgcgcga acttctgcag cagctctttg acttcgttct cgccgcggcc
     1201 ggagcccttc gagatgcgct tcacgcggcc ggtatcgaaa tcggcgcggc ggcgctttgc
     1261 ttgcttgcct gccgtcgtcg cgaagtcttc ccacgacgtc gtcacgaaca cctgcgggtt
     1321 cgtgcgctcc tcgcgcgtca tcgacgagat catcgcttcg atcttcacga gctcgcgatc
     1381 gtcgaggttc acgccttcgg gcagaccgcc gccgaagaac ggcagcttct cgaacagatc
     1441 cttcagcgat cccattttct ggatcaactt gatctgctcg aggaagtcct gcatgtcgaa
     1501 cttgcccttg agcatgcgga gggcgtcttc ttccgccttc tccgcatcga cgacctgctc
     1561 gaaatctttg acgaggccga cgatgtcgcc catgccaagg atgcgcgacg cgagaccttc
     1621 gggccggaac tcctcgagct tgtcgagcga ctcgcccatg ccgaggaact tgatcggctt
     1681 gcccgtgatc tctttgacgg agagcgctgc gccaccgcgc gcgtcgccgt cgagcttcgt
     1741 catgatcacg ccgtcgaggt tcaaccgctt gttgaacgta tcggcgacgt tgaccgcgtc
     1801 ctgacccgtc atcgcgtcga ggacgaggaa gatgttcgca ggagtgaccg acttgttgat
     1861 gtcgtcgagc tcctgcatca gcggctcgtc gatcgcgagg cgaccggcgg tatcgaagat
     1921 gacgacgtcg caaccttcgc gcgccgcagc ttcgttcgct tctttgcaga tcgtgaccgg
     1981 gtctttgccc tcggccgagt agaccggcac gttgagctgg cgaccgagtg tcttcagctg
     2041 ctcgacggcg gcgggacgat agatgtccgc ggcgacgagc atcggcttct tcttgtgcgt
     2101 cttctcgagc cagcgggcga gcttgccgac cgtcgtcgtc ttgcccgagc cctgcagacc
     2161 gcacatcatg atgcccgtcg ggcctttgcc cttcgcccag ttgagctccg tgtcgacggg
     2221 gcccatcatc ttgatcagct cgtcttggca gatcgcgatg aacgcctgct cgggcgtgat
     2281 cgacttgaca ccgtactcgg cgctcttcgc gcgaagctcg acctgcttgc cgcgggcttg
     2341 ctctttcacg cgctcgagga agcgcttgac gacgcggaac tcgacatcgc cttcgagcag
     2401 cgaaagtcga acgtcacgca gcgcttcatc gatgatgtcg tcgttgagct cggcgacacc
     2461 ggtcagccgc tggcgagcgg cacggaatcc cttggcgagg gtctctagca ccgtggtcct
     2521 ataccatctg ccgaggcccg tgagaacagc tggttagacc cgccattccg acccaatgcc
     2581 ccgaatccac gatgcccgcg cacatgcgct ccggaccaag gggtcggggt cctgcgagag
     2641 gtggtcgatg atgagccggt cggccatcag gcggaacggc cattcgagcg catgcgcgat
     2701 accagcccgc cgcgtcggat cggcggagag cgcccatgcc gtggacaggt gcacggcttc
     2761 gatctcgcgc atctcggaga gcgccttcgc gaggctgcgg ccgagcacgg tggccgcatc
     2821 ctgcggatcg atgtgctcgc gatcgatcgg gatgtcctcg tcgagagcca cgtcgagatc
     2881 gatgtcgtcg actgacggag cagacggcgc agcatcgccg gccgccgccg cagcaggcga
     2941 ggccgagagc gcgaggaccg tcagcttcga gagcaggcgg cgcagcgaaa tcatgcgcat
     3001 ttgagtgggc gcaacgtgag gacgcacctc catgccgtca agaccatgcg gcgccaccga
     3061 agcattcccg ccctccccat tcggctatgt ttcgggccga gatatgaagt cgtgcaggca
     3121 gatcgcgctt ctagtagccc tgacagcgtg cggttcgagc tcttccaagc cgacgacacc
     3181 gcaacccgac gtgccaacgc cgacgacgac atccacgcag accgtcgcca ccacaccggc
     3241 gagcaccggg ccgatcgaac cggcgacagc accggcgcaa ggcaaccgct gtgacatggc
     3301 atcggtcggc gagctctcga tcgacggact cgtcgacgat tggaaagcga gccacgtcgt
     3361 cggccgcatc ggatcgccga aggatggcgc ggtcgagctg cactgcgtat gggacggcca
     3421 atcgctcgcg ctgatgatgg acatcaagga cgaccgcctc gtccgcgtga aaggcgggca
     3481 cgaagatcac gtctcgatct cgctcgcggc cggcggcaag cccgtgctcg tcgacatctt
     3541 ccccggcaac tcgatggcga agccgaagat cacgaagccg gcgaaggtcc aagccgcgga
     3601 ctcactccag caaggcggct tctccgtcga ggtcgagatt cctgcttcgg cgattcccga
     3661 cttctcgcca tcgacgcccg cactccagct cgaggcgacg ttccacgatt ccgacgcggc
     3721 gaccggtggc gacgacacgc cgctgcgcct cacgcaaccg atcgagctcg gcgatcgcaa
     3781 agacctcctc gacgatttcc tctcgaccgt ccggctcaag cgcacagaca tcaagctcga
     3841 tcagctcgtc gatctcgatc cggatcatcc gggcaaagag cgcctcgtct acggaggcac
     3901 ggtgatcggc atcctcaccg atgtctacgg ctacgtatcg atgcccgcat ccgccgcggc
     3961 cgacgtgaag aaggtcgagc tgctgccgct cgggcccaaa ggctcacaga tcatcagcgc
     4021 gctcgtgcgg caaaccggca acggcggctc gcgcgatctg ctgatgctct ggacggtgtg
     4081 gagcggtcag ctgcagccgc tcgtcacgat cgaagtgaag aaggagctcg ccggcaacgt
     4141 gctcgagtcg acgtggaaga ttacgaaggg aaagaagggc cccgagctcg tcgtcgagcc
     4201 gaagcccgcg gtcggcttct ccgccgatga ttacgccgag gtcccggccg aggacgccga
     4261 tgcgatcctc ctgccgtggg acgacaagaa agccggcatc gcgtactcgg tgacgggcaa
     4321 agagatcacg cgtcgcgacc tgccgaagaa aaaacgctaa cgcgtacaga cgaatggaaa
     4381 cgtcgccgcg ctacacgacg tgtcgtacca aaggccgtcg gcaaacatga cggcgcattg
     4441 ctcttcgcca tcttccgtac cctgtccatc ctgcggctcg cccgttcccc agttgacgta
     4501 cggcgacgcc actccgtcga gccacgacca gttgccggca acggtcgtcg cgcccgcctg
     4561 ctgcatatag ccgatccacg ccggcgggtt cgtcaattga ctcacgcacg cgtcatcggt
     4621 cgcgtcatgc accgtcgcga gctgaccgcc ccacgcgatg catcgagtgt cagcggtgtc
     4681 ctgcgagacg ctgtccgggc acagcgtgat gcaccggccg tcgcacatca tctgcttcgg
     4741 cgcgccggtg cattgaatgc cagcgcacgc agcgagcacg gcgtccccac tggcatcgcc
     4801 ctgcacggcg tcgcttgcta cgccatcggt tgcgtcgcgt gtgcccgaga ggtcgtcgaa
     4861 tgcgagcctg ccgcatgccg tcgatacgag cgccgcacac acagcccatc gcacacatcc
     4921 acggtaccag cgccaacgag ttagtgcgcg aggatcttct gggcttcacg ctctgcacgt
     4981 ggctggccct tgtaggtctt gaatagctcg agtgcggctt tggcgcgctt gatcgcctgt
     5041 tcgcgcttac cgagcttcga ctcgatttgc gcgacgagga tctcgtcgag ggcgatcaag
     5101 tcgggacgca gcttctgctt gtttgcgatc tcgagcgcct ttgtcccttc gacgagtgct
     5161 tccgcataac gcttctgcac gcggaacgaa tcggccagct tgcgatggaa cgcccattga
     5221 tcgagctcgc cggcgccgag ctcttcgatc gtcacttgta gatcgcgcgc gagcttctcc
     5281 gcttctttgc ctttgccatc gagattgagg aggtcgacgc gccaa
//
LOCUS       DATLBZ010000302        19525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2573436, whole genome shotgun sequence.
ACCESSION   DATLBZ010000302 DATLBZ010000000
VERSION     DATLBZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19525
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2573436"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..446)
                     /locus_tag="VMZ53_15470"
     CDS             complement(78..446)
                     /locus_tag="VMZ53_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29904.1"
                     /translation="MRLVAAFALIGLVGCVTPSIPIPPPDPSKMAFTVETDPNGLVTS
                     ASLSYPATDSYKGGVAYVYNRTLGHGVIEQVNADGSVGPTAPVVATTGNQIVFSVEHD
                     EQTVSTCVLLKDGAPTSYCP"
     gene            complement(566..1087)
                     /locus_tag="VMZ53_15475"
     CDS             complement(566..1087)
                     /locus_tag="VMZ53_15475"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUS29905.1"
                     /translation="MSVDTPPEKPESERQPPVLRREALLPNERSRGLGTVAVVCTMAG
                     VASGLALATTLMAMQVADSMTVRQGPYRVVEVQTVDARPDHGFLGIRYVFRGGAAEID
                     SVLPGTPAQHIGLRRGDHVLAIDGASIRSANDLQERIYDSHPGAQPSLVIERDGSVQT
                     VRPTLTTWPIVSY"
     gene            1293..6536
                     /locus_tag="VMZ53_15480"
     CDS             1293..6536
                     /locus_tag="VMZ53_15480"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HUS29906.1"
                     /translation="MLLVIVSAFAYLRVEWEGPDLGDNIASLLNKRMRGRIAIGSVEW
                     ETSSLKTVLTGGWARVTIRNVKVWDDCALSSNLDPLDERRVGDPSEDCTPDDRPDPDP
                     ASKRKPRKLLLVAPKVTAEIDVHAALFGNHDLIFRHVWVEGGEALLEQTREPYPLHAY
                     DRTIVSFLTAFYPRMKAGFRAGYYAGDAPPKTELRDVHLKDVNVTAHFGEYASSAGVG
                     YTIAARIEGVNVDADIAPGEEDTRSPTTYLAMDATDPVPKFYVRLALKGKHAKLRILD
                     EGARESFVMIGQRMNQAWDAGRNAKYQFELSEIDVNRLAQLPDQWGRKDYVANNLELD
                     ISAHTIPCKVTGTAEQTIKDGADVHLTGQLLEWWDRPYDGKWDLQLAVKNLGPTLRTC
                     VKSTMGGDNLNGTIKLSGPFIADPKVTLDLEGLDFDVPLSKEEEPLRLTLAEVHGDID
                     LVNEQGSIDRTKALIRGGKEPGEVMVAATFQLKPWRVKTSVDITKPIDIGRFLPPKTV
                     AAAGKFLQGKLTAEGDSELGFELKDFDLALGRTEKEKAIVVHRGRLFTDDDFAVIHIQ
                     KVAVDAGRNHAVFDGWVNTEKETDNIDLTIQGCFPELGVWLARFNLPRFAESACNGAA
                     PPAPAQTTGSMQLPQGGVVLVGAYAGAGGQSGSQPTTGNGTTIHITGNYKNPTINLKT
                     TLGGVPCLDNLGVNATFENDVATIHSIVSTGLGGSVSGYGTVLVPQGGLPRIEKLHIE
                     GRNLDSSKLCGLNGLVKGTINTMDVDVRPTVITKKQPMDWLPAFSLQATAKKITVLGE
                     NYSGANICINRNDDSKCRPAWAENRLSPTGKTTCDQAKKANGSCVVVSADRDLGGHFA
                     ATIADVPPQKIGRVAVGRQLGGTVALDDLPLAVIEPLIGKGVMGGLVSATIHLGGDRN
                     APLVEIGSTINLTRAWVAGAYIGDAQLGVIPSTYNNGPAVRLYGSAMAGQIGIDGFIG
                     TAAPYPIDVALRGRRVEVGPFVDLSAKLGSPETLQAWATGTVTVRTELDKKSTKQPEA
                     WVELEEVTAIVDHRTREGRRQPIRFSFVPPGPGAYALSMRVTKTTVELACRTYGVALG
                     RKACPATLVTPAGNVSISGGVTQSQMDLKAHGELDLRRVAPLLENQVEDIAGTLELNG
                     TVSGTFDKPNYDLALDVTKAVTLRLPGGDSVLQVLGPRTQDGEKLSGAHLELKNGSVF
                     FQGFTLNVRDERKDEQGELNIGGSVDFKGLTPARWSMTVDGKIAGKMISALAPSKIAS
                     ATGLARIDGTLSGEGKLPLVDAQLTFDPEEGSRAQPLAIMPRGSGMRRELGMLAGVID
                     ITTKESGTHRTYSVDFRENPLTMTIDNEGKVDNIRGRVVLTDGKIDFASLALDAESIP
                     YRVPGTFDLILSAKNVKLELPSADSAWRASGNVAIVNGNYTRNFLLTEVIRPAPQTIQ
                     PVKPFWSEYPSIGNADLDLAIEVRRFGVANNITPTPIELAGPRLLLRGTPRDPRLSGT
                     IRVQRGEFKIPATRAKFTNTTGSIDFAENEKASNPSLNITSDAPDYVDLSGTAHTITM
                     QISGTLESPAWDLRTSDGYNKSQTLALLFLGRSPEQLRRSLGDQTFGTNDSTRGNTST
                     NPSGGVGDQLVRDLAGDWVSSLLGNSLTKFTRLDVLRFEIGFGSVGISAKKKALDNLD
                     LLGDAEQTIRGYTLNVRGDLRTPFHPLGWVRRKILREVNALSDDRLSGQGGYLRKNFN
                     DPAELDIDDLQAKLVYRLFIP"
     gene            6536..9931
                     /locus_tag="VMZ53_15485"
     CDS             6536..9931
                     /locus_tag="VMZ53_15485"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HUS29907.1"
                     /translation="MKARAFLILAVVARTAAAQPELPPESKSETLEPAQPPGMTTPPA
                     ADPTTRTEADDQAALTPSQVDSASCLPLDLTPGALIRSAQGPSAGPATPIAWTEFEVR
                     GKLVETPATVHALLEPTLNQFRTSLSAATLPQVAQITARFGYQLIGQFTQDLPDGARL
                     VLSLAPLPLVRKVDVGIKDQPFFGKVLDDEIKRRMGIRRGSYLPWEPIRRQCAMLEEK
                     HRIEEFLFDEGYFDASVSIYYVPRDVGYADVRVTVELNEKYEVANVKVTCPPGSEARK
                     RQCFDTSTNERVELPIPLPEIVEIFEHKRTCFILGICRGGGYSRTQYQKDIQAVKERF
                     QTLGYAGVRVQASDPRTTINRDRKTVNPIITIDPRRRVEVDFEGYDRDAFNLSSLRKQ
                     LTFNDAASADDVEAMDSARALTTYLQTRGYFDARVTFTRERVDVEAKPDSNDVGIHYD
                     RIRFQIDLGNIRRVARVEFVGNKAIGSDRLRELLATKVATGAGSLFGTTAAATSAELI
                     LDQERIKEAYRQAGYPNATVWPSASVRTAGLDNAALTAALLGVDDGNSLVVRFTIDEG
                     EPTLLTRIVIVGDGDKPIDDALCADVLGELATELHEREVARRSDPTQCVSTISGLKFR
                     YDDIAGTRDRLREYLLKLGRGRAIVDYVAVPFGINRVEARYTIRRVQRLKVGKIIVRG
                     AVRTADRLITGELGFAEGDVLTTDELAEGARKLRNTGLFEAVNIDMPDLDCGENEEGN
                     CNSDTINAIVRVEERYLHRAEVGVEGGLSSQNGYFGTLRWTQGNLFGHGILFRFSGTY
                     GSKLSEVEGTFRLPQWIVRRRDDRFGVPESIAFATEVTGLYRQQQTERFGELTTKGFT
                     VGIARQWPQPRTETEAAHSYGLGLAYDYRVRIRNVDALRPIGADMDQSQVAISTTTGQ
                     LRVTFEIEKRIDRSGQLAPLAPEDGHRLELSASYASTHLLGQDDFIKFYAYGAKFFPI
                     GSNLVLRFDGRYDQGYPIGEDVLLPEVERLFAGGDSTVRGYSEDRLKTEIIEVGVPPL
                     DNVSQIRIIPVGGNIRALASVDAQVRIFKILAGAVFSDAGVVTNSWRTTTVDDIRPSV
                     GMGLRALTPFGIGALEYAVPIRPHLGDDPRGRIHFYFAARAQF"
     gene            9949..10086
                     /locus_tag="VMZ53_15490"
     CDS             9949..10086
                     /locus_tag="VMZ53_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29908.1"
                     /translation="MGRGDNRKTWKMRRKKRQQKLKRRTKRKIETAKAAKKPAEKPKK
                     K"
     gene            10099..10575
                     /locus_tag="VMZ53_15495"
     CDS             10099..10575
                     /locus_tag="VMZ53_15495"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HUS29909.1"
                     /translation="MTPEQTRCVMTQIVMPMHTNGMSGVMFGGIMMQWIDVCAGVAAM
                     RHASGAVLTASIDRLDFLSPVKVGEIVVLQAQVNYAGRTSMEVGCRVETEDMRTGTRR
                     YVTKSYLTFVAVDGEGKPRTIPALELVTDEDKRRHEDALTRRSERLKAAGRSGGKP"
     gene            10572..11348
                     /locus_tag="VMZ53_15500"
     CDS             10572..11348
                     /locus_tag="VMZ53_15500"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29910.1"
                     /translation="MTITIDRSDWGRLRVTGADRVRFLQGLTTVNVETLADGAHAWGA
                     ILNPKGRVLTVIDIGMVGEAILIASEPSLADKTRQLLEKYAVMDEVTFEPLAGPAHQI
                     WDDPVDVWRSPIVEGAAPDAIASSDPQVERLRIRAGFVRYGVDVDEDCFPFETPLAQF
                     LDYKKGCYVGQEPVFRVHSQGNTARILRGLVVEGDAPIAAGTQIKHATKDKAGELTSS
                     VAAGDGSTLALGYLHRTVWDVGGTVEIDGRRATVHELPWT"
     gene            11351..12631
                     /locus_tag="VMZ53_15505"
     CDS             11351..12631
                     /locus_tag="VMZ53_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29911.1"
                     /translation="MNRAALLLLAASGCDYVTSSLKTNDFSGDAYPILVDRSAGGVVV
                     GMREGSSDRTAVLDVMSPLTLIDKGGDVRVSIAERDLTLLGARSLGAALDLPRARFPE
                     KTVATLHPCQDATCSIGTPGALRPFDALFGLDSFAGDALRLRLGDDQIFVLPDIAGSE
                     VRRGRACDAFFESPYRGGGTLVIAGTELDFSNFRVAIDTCIAPNPIRDTPQSKRGADA
                     LLVASTAIGISILDESTYERYRELDSATIPDLATLPDDTVYLPSGPITGKLASIPSLA
                     LVSNLSANPRAPCRQVWASHLMAARDCAPGDDCPCTGGSTYCSAPAIVELAPAAPATT
                     VPILVVSDAAPILQALRAELRPDRPEVDGILGADILTSVELDLDYTHDRMLARCTNRD
                     ACSTRTFINGDTDERDSSRFYVNGCLGDRRPPDP"
     gene            complement(12632..13327)
                     /locus_tag="VMZ53_15510"
     CDS             complement(12632..13327)
                     /locus_tag="VMZ53_15510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS29912.1"
                     /translation="MADLQAKIQLSRSAEWKLDVDLACPAGITCIMGPSGSGKSTILG
                     VLAGLTVPDAGSVKVGNEVWLDRAKGIDVPIHRRRLSYVFQGLALFPHMSALHNVTYG
                     MAHVPREQRAERAKTLLQKLGVLHLAMRRPRTFSGGEAQRVALARALARQPELVLLDE
                     PFSALDRELRTQLIALVREVVADLGVPIVHVTHSPGEARALADQIVRLEKGRVVARGT
                     PAEVLKGVTSLEE"
     gene            complement(13320..13988)
                     /gene="modB"
                     /locus_tag="VMZ53_15515"
     CDS             complement(13320..13988)
                     /gene="modB"
                     /locus_tag="VMZ53_15515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HUS29913.1"
                     /translation="MTWDPLILSLKVATLATLLTLVVGTGLALLLTWRRLPAANLFDA
                     IVSAPLVLPPTVLGYYLLVALGTESAVGRAWERMTGTTIVFTFTGAVIAAAVGSLPLV
                     VRSIRVGLESVDPNLISAARTLGASPGRVTLTVTLPLAAPGLIAGAMLGFARALGDYG
                     ITQMVAGSRINGTPTASIYVMDAMVANREHEARMMAIATTIVGVALLYIANRLTRRIG
                     PYRG"
     gene            complement(13994..14704)
                     /locus_tag="VMZ53_15520"
     CDS             complement(13994..14704)
                     /locus_tag="VMZ53_15520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS29914.1"
                     /translation="MTVDPNAELREKKRVLAALVVHDLRSPLSAVQGYLHLLREEVGD
                     DASHVVKQYIDDANLLLGKALSLVATILDVDELEDGILRAMKSPVKITELIERARAGN
                     RAHFEVRQLRVDVDVDPEMTVQIDRDLFSRVIENMLDNAARYAPRGGRCSVAVKRDDQ
                     GVEIAIGNSGPPVPPAERDKIFGRYFQVERRRASARANRGLGLYFCKLAVEAHGGTIH
                     VEERGELGAVFVVRLPPD"
     gene            complement(14718..15602)
                     /locus_tag="VMZ53_15525"
     CDS             complement(14718..15602)
                     /locus_tag="VMZ53_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HUS29915.1"
                     /translation="MSDTGRNKTVVTAISKISDRPVAKEACLVVIYGLELGKKFNLNR
                     PQIIIGRSSKADIQIDQEAVSRNHCKIINTGNAIMLRDMGSTNGTYVNDEMIDEYVLR
                     DGDFIKVGRCIFKFLSGNNIENAYHEEIYRLTTVDGLTQVFNRRYFVETLEREIGRAL
                     RYRRDLSLIMFDIDRFKGVNDSFGHLAGDHVLKHLASVIKTRIRREDVLARYGGEEFA
                     IVLPEIDHYNAMQFSEKIRKLVEVAEFKFEDAVIPVTISIGVASLRGEIEDALEFIKQ
                     ADTHLFSAKENGRNRVVG"
     gene            complement(15635..16003)
                     /locus_tag="VMZ53_15530"
     CDS             complement(15635..16003)
                     /locus_tag="VMZ53_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29916.1"
                     /translation="MSNSPKVDHADRDLDNDGRAEIIVVDRAQCTSDGNCYWNVFRQP
                     RDPSDCARYAGTFAGANLEPLASRGEDNMTDVRSYWQQSGGRMLLQSYRYVREGYRIE
                     DVLQCKRAPDDRLDCADTNR"
     gene            16212..17507
                     /locus_tag="VMZ53_15535"
     CDS             16212..17507
                     /locus_tag="VMZ53_15535"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HUS29917.1"
                     /translation="MRRVVLLAGGVLLAGASVFVNAGPDHKPKKPTPKASIQGESLVA
                     APSGPITTPSQLSVDTKTSDVLSDLQLDKLTLEDGHYVAPLKDGRRAVLTLDPDLQKL
                     AEKLLDQSRAPRGAIVAMSPDGRILALAGRRTEEPKGGKTGTFDYKLATDVWAPAASV
                     FKLVTATSLVANGYDPSEKVCYHGGVRSVMESNLSDSKSDNNCETLAYGVAHSNNAIL
                     GKLAFQKLEPSLLDRTARDLGWASKQELGVEATCGELALPEKKDLEFAKAAAGFQGSR
                     LSVLGGAMLVSTFAGKGEQPQPRIIDSIAGKPVDPGKPHRVIDEKVAEAVTKMMVGTC
                     ASGSAAKSFRRSDNNVAGKTGTLTTNKPFYMEHSWFVGFAPADKPEIIVSVLLGNPES
                     WHLRGHEAAKRMIDRALSAKSSAPDLSDEPSKSAAKRPK"
     gene            17563..18681
                     /locus_tag="VMZ53_15540"
     CDS             17563..18681
                     /locus_tag="VMZ53_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29918.1"
                     /translation="MLASSLLATHAFAETQAEIAARENEEGKQLMFENKYSEASAKFM
                     SAHSRVPEPKYFFNHCTSLFWEGKFGESLTACNAADKNADDKLKAKIAAFEDKIKAEA
                     QKQGVVLQPVGGGAGPGETPPVGTDPNNPNGNPPGPGPTGPSGQPPPSYGGVGRAPSM
                     GLFSQSKPENKYAWSLGIDIFGGGGRIGQPGYFGHAAGGFRLKSDYLLNPKMRLGTQG
                     YFQISNYGNGDEMGAQGAYSLGIFDLGIAGYKHICGPHSRFCLTPLVGVQLALLSPAN
                     QTDSTGDTVFNYASLGARLEMAASLMLGSRYQHVISGIIGLNAYTQAFSEPADCSPGF
                     DCKEDWGLDKGGAAFYLGAGYTYRFSTPFGSAPFVTLE"
     gene            18719..>19525
                     /locus_tag="VMZ53_15545"
     CDS             18719..>19525
                     /locus_tag="VMZ53_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HUS29919.1"
                     /translation="MAGPEKLSAKLSLPGGKEATLPVIVGTEDEHAVDIRSLRQDTGF
                     ITLDSGYMNTGSTTSAITYLDGEQGILRYRGYSIEDLAEKSDFVETSYLLIHGELPTK
                     QQRETFSKNLTRHSLIHEDMHHFFAGYPPTSHPMAVLSSMVTSLSAYYPDSLDRNSAI
                     DLHITRILSKVRTIAAFAYKKSIGQPLIYPKNSLRYCANFLHMMFAVPSEEYEVSPIL
                     EKAVNQLLILHADHEQNCSTSTVRMVGSSGASIYAAIASGILALWGPLHGG"
BASE COUNT         3570 a         6479 c         6286 g         3190 t
ORIGIN      
        1 tcggtcaagc gcacgccgta agcgccgcgc tctcgatcga accgagcggc gccgcctggc
       61 gccgtttgca ttttcggcta cgggcagtag ctcgtcggtg cgccgtcttt gagcagcacg
      121 cacgtcgaca cggtctgctc gtcatgctcg accgagaaca cgatctggtt tccggtggtc
      181 gcgacgaccg gggccgtcgg accgacgctg ccatccgcgt tgacctgctc gatcacgccg
      241 tggccaagcg tccggttgta gacgtaggcg acgccgccct tgtagctgtc cgtggccgga
      301 tagctgagcg acgcggacgt gaccaggcca ttggggtcgg tctcgaccgt gaacgccatc
      361 ttggatgggt cgggcggcgg aatcgggatc gagggcgtga cacagcctac gagtccgatc
      421 agcgcgaatg cggcaacgag acgcatgaat ccgggtaggg ctacagcaag ccctgatcca
      481 gatgagcgta caggatcttc aacagttacg aaatcagccc gccaatggcg ttggcaggct
      541 gccaagactg ttgaggtctc gcgtcctagt agctgacgat cggccaggtc gtgagcgtcg
      601 gccgcaccgt ctggacgctg ccatcccgct cgatcacgag cgagggctgc gcaccgggat
      661 gcgagtcgta gatgcgctcc tgcagatcgt tcgcggagcg aatcgacgcg ccgtcgatcg
      721 cgagcacgtg gtcaccgcgg cgaagaccga tgtgctgcgc gggcgtgccg ggcagaacag
      781 aatcgatctc ggcggcgcca ccgcggaaca cgtagcggat gccgaggaag ccgtgatccg
      841 gccgtgcgtc gaccgtctgg acttccacga cccgatacgg gccctggcgt accgtcattg
      901 aatcggcgac ctgcatcgcc atcaacgtgg ttgcgagcgc gaggcccgag gcaacaccgg
      961 ccatcgtgca cacgacggcg acggtgccga gtcctcggct gcgttcgttg gggaggagtg
     1021 cttcccggcg caggacggga ggctggcgtt cggactcggg cttctcgggc ggcgtatcga
     1081 cagacatctc gatccctcgc tttcggtcct tactcccagt gtacgcaacg acgatccgat
     1141 tgccatcgag aagtgcgggt cgtgcgtcga cgccacagga aactcgctga tcgcctgaag
     1201 cgttgaacat caaacgtcgt gatagcgtcc ggagcacgtt gggacggtcc cgccgacgca
     1261 agcgccggta cctcgtcgtt actggctttg cggtgctgct cgtcatagtc agcgccttcg
     1321 cgtacctgcg cgtcgagtgg gaggggcccg acctcgggga caacatcgcg tccctcctca
     1381 acaagaggat gcgcggtcgc atcgcgatcg ggtcggtcga atgggagacg tcctccctga
     1441 agacggtact cacgggcggc tgggcacggg tcaccatccg caacgtaaag gtttgggacg
     1501 actgtgctct ttcgtccaat ctcgatcccc tcgacgaacg gcgggtcggc gaccccagcg
     1561 aggactgtac gcccgatgac aggcccgacc cggacccggc ctccaagcgc aagccgcgca
     1621 agctgctcct cgtcgcgccg aaggtcacgg cggagatcga cgtgcatgcc gcactgttcg
     1681 gcaatcacga tctgatcttc cggcacgtgt gggtcgaggg cggcgaggcc ctcctggagc
     1741 agacccgcga gccctacccg ctccacgcct acgaccggac gatcgtgagc ttcctcaccg
     1801 cattctaccc gcggatgaag gcgggcttcc gcgcggggta ctacgccggg gatgcgccgc
     1861 ccaagaccga gctccgcgac gtccacttga aagacgtcaa cgtcacggcg cactttggcg
     1921 agtacgcgtc gagcgccggc gttggctaca cgatcgccgc gcgcatcgaa ggcgtgaacg
     1981 tcgatgccga catcgctccg ggtgaggagg atacgcgctc ccctacgacg tatctcgcga
     2041 tggacgcgac ggaccccgtc ccgaaattct acgtacgcct cgcgctcaag ggcaagcacg
     2101 cgaagctccg catcctcgac gaaggcgcgc gtgaatcgtt cgtgatgatc ggccagcgca
     2161 tgaatcaggc gtgggacgcc gggcgcaacg cgaagtacca gttcgagctg tccgagatcg
     2221 acgtcaaccg gctcgcgcag ctgcccgatc aatggggccg caaggactac gtcgccaaca
     2281 acctcgagct cgacatcagc gcgcacacga ttccgtgcaa ggtcacgggc acagccgagc
     2341 agacgatcaa ggacggcgcc gacgtccatc tcaccggtca gctactcgag tggtgggacc
     2401 gcccgtacga cggtaagtgg gacctccagc tcgcggtgaa gaacctcggg cccacgctgc
     2461 gaacctgcgt gaagtcgacg atgggcggcg acaacctcaa cggcacgatc aagctgtcgg
     2521 gcccgttcat cgctgatccg aaggtcacgc tcgatctcga gggcctcgac ttcgacgtcc
     2581 cgctgtcgaa ggaagaagag ccgctgcggt tgacgctcgc cgaggtccac ggcgacatcg
     2641 atctcgtcaa cgagcagggc tcgatcgatc gcacgaaggc gctgatccgc ggtggcaagg
     2701 aacccggcga ggtgatggtc gccgcgacgt tccagctcaa gccgtggcgg gtgaagacga
     2761 gcgtcgacat cacgaagcca atcgacatcg gccggttcct gccgccgaag acggtcgcag
     2821 ccgccggcaa gttcctgcaa ggcaagctca ccgcggaggg cgacagcgag ctcggcttcg
     2881 agctgaagga tttcgatctc gcgctcggcc gcaccgagaa agagaaggcg atcgtcgtcc
     2941 atcgcgggcg gctgttcacc gacgacgatt tcgcggtcat tcacatccag aaggtcgccg
     3001 tcgatgcggg ccgcaaccac gccgtgttcg atggctgggt caacaccgag aaggagaccg
     3061 acaacatcga tctcacgatc cagggctgct tcccggagct cggcgtgtgg ctcgcgcggt
     3121 tcaacctgcc gcggttcgca gagagcgcgt gcaatggggc cgcgccgccc gcacccgcgc
     3181 agaccaccgg ttcgatgcag ctgccgcaag gcggtgtcgt gctcgtcggt gcgtatgcgg
     3241 gcgccggcgg ccagagcgga agccagccga cgaccggcaa cggcacgacg atccacatca
     3301 ccggcaacta caagaatccg acgatcaacc tgaagaccac gctcggcggc gttccgtgcc
     3361 tggacaacct cggcgtcaac gcgacattcg agaacgacgt cgcgaccatc cactcgatcg
     3421 tgtcgaccgg tctcggtggc tccgtctccg gctatggaac cgtgctcgtg ccgcagggcg
     3481 gattaccgcg catcgagaag ctgcacatcg aaggccgcaa cctcgacagc agcaagctgt
     3541 gcggcctgaa cggcctcgtc aaaggcacga tcaacacgat ggatgtcgac gtgaggccga
     3601 ccgtcatcac gaagaagcag ccgatggact ggcttcccgc gttctcgctg caggcgacgg
     3661 cgaagaagat cacggtgctc ggtgagaatt actccggcgc gaacatctgc atcaatcgca
     3721 acgacgacag caagtgccgc cctgcgtggg ccgagaaccg cctctcgccg acgggcaaga
     3781 cgacgtgtga tcaggcgaag aaagcgaacg gctcgtgcgt cgtcgtgagc gccgaccgcg
     3841 atctcggtgg ccacttcgcc gcgaccatcg ctgatgtccc gccgcaaaag atcgggcggg
     3901 tcgcggtcgg acgccagctc ggtggcaccg tcgcgctcga tgatcttccg ctcgccgtga
     3961 tcgagccgct gatcggcaaa ggcgtcatgg gcgggctcgt gtcggcgacg atccacctcg
     4021 gcggcgatcg caacgcgccg ttggtcgaaa tcggctcgac gatcaatctg acgcgcgcgt
     4081 gggtcgccgg cgcctatatc ggtgacgccc agctcggcgt gatcccgtcg acgtacaaca
     4141 acggcccggc cgtgcgcctc tatggctcgg cgatggccgg ccagataggc atcgatggat
     4201 tcatcggcac ggcagcgccg tacccgatcg atgtcgcgct gcgaggacgg cgcgtcgagg
     4261 tcggcccgtt cgtcgacctc agcgcgaagc tcggctcgcc cgagacgctg caggcgtggg
     4321 cgacgggcac ggtcaccgtc cgcaccgagc tcgacaagaa gagcacgaag cagcccgagg
     4381 catgggtcga gctcgaagaa gtcaccgcga tcgtcgacca ccgcacgcgc gaaggccggc
     4441 gccagccgat ccggttctcg ttcgtgcccc cgggccccgg cgcgtatgca ttgtcgatgc
     4501 gcgtcacgaa gaccaccgtc gagctcgcgt gccgcacgta tggcgtcgcg ctcggccgca
     4561 aggcctgccc cgcgacgctc gtcacgccgg caggtaacgt ctcgatctcc ggcggcgtca
     4621 cgcagtccca gatggacctc aaggcgcacg gcgagctcga ccttcgccgg gtggcgccgc
     4681 tgctcgagaa tcaagtcgag gatatcgccg gcaccctcga gctcaacggc acggtcagcg
     4741 gcacgttcga caagccgaac tacgatctcg cgctcgacgt gacgaaggcg gtcacgctcc
     4801 gtctccccgg cggcgactcg gtgctccagg tcctcggccc gcgcacgcag gacggcgaga
     4861 agctgtcggg cgcgcacctc gagctcaaga acgggtccgt gttcttccag ggcttcacgc
     4921 tgaacgtgcg cgacgagcgc aaggacgaac aaggcgagct caacatcggt ggctccgtcg
     4981 atttcaaggg gctcactccg gcgcgctggt cgatgacggt cgacggcaag atcgccggca
     5041 agatgatctc cgcgctcgcg ccgagcaaga tcgcatcggc gacgggcctc gcacgcatcg
     5101 atggcacgct gtcgggcgag ggcaagctgc cgctcgtcga cgcgcagctg acattcgacc
     5161 cggaagaggg ctcgcgcgcg cagccactcg cgatcatgcc gcgcggctcg ggcatgcgtc
     5221 gcgagctcgg catgctcgcc ggcgtcatcg acatcacgac gaaagaatcg gggacgcatc
     5281 gcacgtacag cgtcgacttc cgcgagaacc cgctgacgat gacgatcgac aacgagggca
     5341 aggtcgacaa catccgcggc cgcgtggtgc tcaccgacgg caagatcgat ttcgcgagcc
     5401 tcgcactcga tgccgagagc attccgtatc gcgtgcccgg cacgttcgat ctgatcctca
     5461 gcgcgaagaa cgtcaaactc gagctgccga gcgcggactc ggcgtggcgc gcgtccggca
     5521 atgtcgcgat cgtcaacggc aactacaccc gcaacttcct cctcaccgag gtgatcaggc
     5581 cggctccgca gaccatccag ccggtcaagc cgttctggtc ggagtacccg tcgatcggca
     5641 acgcggatct cgatctcgcg atcgaagtga ggcggtttgg cgtcgcgaac aacatcacgc
     5701 cgacgccgat cgagctcgcc ggtcctcgcc tgctgctgcg tggcacgccg cgcgatccgc
     5761 gactgtcggg cacgattcgc gtccagcgcg gcgagttcaa gattcctgcg acgcgcgcga
     5821 agttcacgaa cacgacgggc tcgatcgact tcgccgagaa cgagaaggcg agcaatccgt
     5881 cgctgaacat cacgagcgac gcgcccgact acgtcgatct cagcgggacc gcgcacacga
     5941 tcacgatgca gatcagcggc acgctggaga gcccggcgtg ggacctgcgc acgtcggatg
     6001 gctacaacaa atcgcagacg ctcgcgctgc tgttcctcgg ccgcagcccc gagcagctcc
     6061 gccgatcgct tggcgatcag acattcggca ccaatgattc cacgcgcggc aacacgtcga
     6121 cgaatccgtc gggtggcgtc ggcgatcagc tcgtgcgcga tcttgccggc gactgggtgt
     6181 cgagcctgct cggcaactcg ctcacgaagt tcacgcgcct cgacgtcctt cgcttcgaga
     6241 tcgggtttgg ctccgtcgga atctcggcaa agaagaaggc gctcgacaac ctcgatctgc
     6301 tgggcgacgc cgaacagacg atccgcggtt acaccctcaa cgttcgcggc gatctgcgga
     6361 cgccgtttca tccgctcggc tgggtccggc gcaagattct cagggaagtg aatgcgctga
     6421 gcgacgaccg gctcagcggc cagggcggtt accttcgcaa gaacttcaac gaccctgccg
     6481 agctcgacat cgacgatctc caggcgaagc tcgtctatcg actgtttatc ccgtgatgaa
     6541 ggctcgcgcg ttcctgattc tcgccgtcgt ggcgcgtacc gccgccgcgc agcccgagct
     6601 gccgcccgag tcgaagtccg agacactcga gcccgcgcag cctccgggga tgacgacgcc
     6661 accggcggca gatccgacaa cgaggaccga agcagatgac caggcggcgc tgacgccgag
     6721 ccaggtcgac agcgcgtcgt gcttgccact cgatctgacg cccggcgcgc tgattcgttc
     6781 tgcgcaaggc ccgagcgcgg ggccggcgac gccgatcgca tggacggagt tcgaggtacg
     6841 cggaaagctc gtcgagacac ccgcgacggt ccatgcgctc ctcgagccga cgctcaatca
     6901 gttccgtacg tcgctatctg cagcgacgct gccgcaggtc gcgcagatca ccgcgcgctt
     6961 cggctatcag ctcatcggcc agttcacgca ggacctgccg gacggcgcgc gcctcgtcct
     7021 ctcgctcgct ccgctgccgc tcgtgcgcaa agtcgacgtc ggcatcaagg accaaccatt
     7081 tttcggcaag gtcctcgacg acgagatcaa acgccgcatg ggcattcgcc gcggctcgta
     7141 cctgccgtgg gaacccattc gacggcagtg cgcgatgctc gaagagaagc atcgaatcga
     7201 agagttcttg ttcgacgagg gatacttcga cgcgagcgtc agcatctact acgtgccgcg
     7261 tgatgtcggg tacgcagacg tccgtgtcac cgtcgagctc aatgaaaagt acgaggtcgc
     7321 gaacgtcaag gtgacgtgcc ctcccgggag cgaggcgcgc aagcgccagt gcttcgacac
     7381 atcgaccaac gagcgcgtcg agctgccgat tccgctaccg gagatcgtcg agatcttcga
     7441 gcacaagcgc acgtgcttca tcctcggcat ctgtcgcggc ggcggttact cgcgaaccca
     7501 gtatcagaag gacatccagg ccgtcaaaga gcgctttcag acgctcggct atgcgggcgt
     7561 gcgcgtgcaa gcgagcgatc cgcgcacgac gatcaatcgc gaccgcaaga cggtgaaccc
     7621 catcatcacg atcgatccac ggcggcgcgt cgaggtcgac ttcgaaggct acgaccgcga
     7681 cgcgttcaac ctatcgagcc tgcgcaagca gctcaccttc aacgatgcgg cgagcgccga
     7741 cgacgtcgag gcgatggact ccgcgcgcgc gctcacgacg tacttgcaga cgcgcggcta
     7801 cttcgacgca cgcgtcacgt tcacgcgcga acgcgtcgac gtcgaagcga agccggacag
     7861 caacgacgtc ggcattcact acgatcgcat ccgcttccag atcgacctcg gcaacatccg
     7921 gcgcgttgcc cgcgtcgagt tcgtcggcaa caaggcgatc ggcagcgaca ggctgcgaga
     7981 gctgctcgcg acgaaggtag cgaccggcgc gggctcgctg tttggcacga ccgccgccgc
     8041 gacgagcgcc gagctcatcc tcgatcagga gcgcatcaaa gaggcgtatc gccaggcggg
     8101 ctatccgaat gcgacggtat ggccaagcgc gagcgtgcgc accgccgggc tcgacaatgc
     8161 ggcactgacc gccgcgctgc tcggtgtcga cgacggcaac tcgctcgtcg tgcgcttcac
     8221 gatcgatgaa ggcgaaccga cgctgctgac gcgcatcgtg atcgtcggcg acggagacaa
     8281 gcccatcgac gacgcgctgt gtgcggacgt cctcggcgag cttgcaaccg agctccacga
     8341 gcgcgaagtg gcgaggcgct cggacccgac gcaatgtgtc tcgacgatca gcgggctcaa
     8401 gttccgctac gacgacatcg ccggcacgcg cgatcggctg cgcgagtatt tgctgaaact
     8461 cggtcgcggt cgcgcgatcg tcgattacgt cgcggtgccg ttcggcatca accgcgtcga
     8521 agcgcgctac acgatccggc gcgtccaacg gctcaaggtc ggcaagatca tcgtgcgcgg
     8581 ggctgtccgg acggccgatc gtctgatcac aggagagctc ggcttcgcag aaggcgatgt
     8641 gctgactacc gacgagctcg ccgagggtgc gcgcaagctc cgcaacacgg gcctcttcga
     8701 agcagtcaat atcgacatgc cggacctcga ttgcggcgag aacgaggagg gcaactgcaa
     8761 cagcgacacg atcaacgcga tcgtgcgcgt cgaagagcgc tatctgcatc gtgcagaggt
     8821 cggcgttgaa ggtggtctgt cgagccagaa cggttacttc ggcacgctgc gctggacgca
     8881 gggcaaccta tttggccacg gcatcctgtt ccgcttctcg ggcacgtacg gcagcaagct
     8941 ctccgaggtc gagggcacgt tccggctgcc gcaatggatc gtccggcgtc gcgatgatcg
     9001 ctttggcgtg cccgagagca tcgcattcgc gaccgaggtc accgggcttt atcgccagca
     9061 gcagaccgag cgcttcggcg agctgacgac gaagggcttc accgtcggca tcgcgcggca
     9121 atggccgcaa ccgcgcacgg agaccgaagc tgcgcattcg tatggactcg gtctcgcgta
     9181 cgactatcgc gtgcgtattc gcaacgtcga tgcgctgcgc ccgattggcg ccgacatgga
     9241 ccaatcgcag gttgcgatct cgacgacgac gggccagtta cgcgtcacgt tcgaaatcga
     9301 aaagcgcatc gaccgctcgg gtcagctcgc gccgctcgcg ccggaagatg gtcatcggct
     9361 cgagctgtca gcctcctacg cgtcgacgca tctgctcggc caggacgact tcatcaagtt
     9421 ctatgcgtac ggcgcgaagt tcttcccgat cggctcgaac ctcgtcctgc gcttcgatgg
     9481 ccgctacgac cagggctatc cgatcggcga ggacgtgctg ctgcccgagg tcgagcgcct
     9541 gtttgccggt ggcgattcga ccgtgcgcgg ctactcggaa gaccggctca agaccgaaat
     9601 catcgaggtc ggcgtccctc cgctcgataa cgtgtcgcag atccgcatca tccccgtggg
     9661 cggcaacatc cgcgcgctgg cctcggtaga tgcccaggtc cgcatcttca agatcctcgc
     9721 gggagccgtg ttctccgacg ccggcgtcgt cacgaacagc tggcggacga ccaccgtcga
     9781 cgatatccgg ccgtccgtgg gcatgggcct gcgcgccctg accccgtttg gcatcggtgc
     9841 gctcgagtac gccgtcccga tccggcctca tttgggcgac gatccgcgtg gaagaatcca
     9901 cttctacttt gctgcgcgcg cccagttctg agttataagc agcgccctat gggacgcgga
     9961 gacaatcgca agacctggaa gatgcgccgc aagaagcgcc agcagaagct gaagcgccgc
    10021 acgaagcgga agatcgagac ggccaaggcc gccaagaagc ctgccgagaa gccgaagaag
    10081 aagtaaggtt cgggggtcgt gacccctgag caaacgcgct gcgtgatgac gcagatcgtg
    10141 atgccgatgc atacgaacgg tatgtccggc gtcatgttcg gcggcatcat gatgcagtgg
    10201 atcgacgtct gtgctggcgt cgcggcgatg cgccatgcgt cgggcgcggt gctgaccgcc
    10261 tcgatcgatc ggctcgattt tctatcgccg gtaaaggtcg gcgagatcgt cgtgctgcag
    10321 gcgcaggtca actacgccgg acgcacgtcg atggaagtcg gctgccgcgt cgagaccgag
    10381 gacatgcgga cgggcacgcg ccgctacgtg acgaagtcgt acctgacgtt cgtcgcggtc
    10441 gatggcgagg gcaagccgcg gacgattccc gcgctcgagc tcgtgaccga cgaggacaag
    10501 cggcggcacg aggatgccct cacgcgtcga agcgagcgcc tcaaagcggc gggccgcagt
    10561 ggaggcaagc catgacgatt acgatcgacc gttccgactg gggacgcctg cgcgtgacgg
    10621 gggcggaccg cgtgcgcttc ctgcagggcc tcacgaccgt caacgtcgag acgcttgccg
    10681 acggcgcgca tgcgtgggga gcgatcctca atcccaaggg ccgcgtgctc accgtgatcg
    10741 acatcgggat ggtgggcgag gcgatcctga tcgcgtcgga gccttcgctc gctgacaaga
    10801 cgcgccagct gctcgagaag tacgccgtga tggacgaggt caccttcgag ccgctcgccg
    10861 ggcctgcgca tcagatctgg gacgaccccg tcgatgtctg gcgctcgccg atcgtcgagg
    10921 gcgcggcgcc cgacgcgatt gcatcgtcgg atccgcaggt cgagcggctg cgcattcgtg
    10981 cgggcttcgt tcgttacggc gtcgatgtcg acgaagattg cttcccgttc gagacgccgc
    11041 tcgcgcagtt cctcgactac aagaagggct gctacgtcgg ccaggaaccc gtgttccgcg
    11101 tccactcgca aggcaacacc gcgcgcatcc tgcgcggact ggtcgtcgaa ggcgatgcgc
    11161 cgatcgcagc aggcactcag atcaagcacg cgaccaaaga caaggccggc gagctgacct
    11221 cctctgtcgc ggccggcgat ggctcgacgc tcgcgctcgg ctatctgcac cgcaccgtgt
    11281 gggatgtcgg cggcaccgtc gagatcgatg gccgtcgcgc gacggtgcac gagctcccct
    11341 ggacatgacc gtgaatcgcg ccgccctcct cctcttggcg gcgagcggct gcgactacgt
    11401 cacgtcgagc ttgaagacga acgacttctc cggcgacgcg tatccgatcc tcgtcgatcg
    11461 cagcgcgggt ggcgtcgtcg tcggcatgcg cgaaggctcg agcgatcgca cagcggtgct
    11521 cgacgtgatg tcgccgctca cgctgatcga caagggcggc gacgttcgcg tctcgatcgc
    11581 cgagcgcgat ctgacgctcc tcggtgcacg ctcgctcggc gcagcgctcg acctcccgcg
    11641 cgccaggttt ccggagaaga cggtcgcgac actacatcca tgtcaggacg cgacgtgctc
    11701 gatcggcacg cccggcgcgc tgcgtccgtt cgacgcgctg ttcggcctcg actcgtttgc
    11761 aggcgacgca cttcgcctcc gcctcggcga cgaccaaatc ttcgtgctgc ccgacatcgc
    11821 aggcagtgag gtccgtcgcg ggcgcgcatg cgatgccttc ttcgaatcgc cgtaccgcgg
    11881 cggcggcacg ctcgtcatcg cagggaccga gctcgacttc tcgaacttcc gcgtcgcgat
    11941 cgacacgtgc atcgcgccga acccgatccg cgatacgccg cagtccaagc gcggcgcgga
    12001 cgcgctcctt gtcgcctcca ccgcgatcgg catctcgatc ctcgacgagt cgacgtacga
    12061 gcgctaccgc gagctcgaca gcgccaccat cccggacctc gcgacgctcc ccgatgacac
    12121 ggtctacctg ccgtccgggc ccatcacagg caagctcgcg agcatcccga gcctcgcgct
    12181 cgtcagtaat ctctccgcga atccacgcgc gccgtgccgc caggtctggg cgagccactt
    12241 gatggccgct cgcgactgcg caccgggcga cgattgtccg tgcaccggcg gcagcacgta
    12301 ctgctcggca cccgccatcg tcgagctggc gccggccgcc ccggcgacga cagtcccaat
    12361 cctcgtcgtc tccgacgccg cgccgatcct gcaagcgctg cgcgccgagc tgcgacccga
    12421 tcgccccgaa gtcgacggca tcctcggcgc cgacatcctc acttccgtcg agctcgacct
    12481 cgactacacg cacgaccgca tgctcgcgcg ctgcacgaat cgcgatgcgt gctcgactcg
    12541 cacgttcatc aatggagata cggacgagcg tgactcgtcg cgcttctacg tcaacggctg
    12601 tctcggcgac cggcgcccac cggatccgta gctactcctc gagcgacgtc acgcctttca
    12661 gcacctcggc cggcgtaccg cgcgcgacga ccctgccctt ctcgagccgc acgatctgat
    12721 cggcaagggc acgcgcttct cccggcgagt gcgtgacgtg cacgatcggc acgccgagat
    12781 ccgcgacgac ctcgcgcacg agcgcgatca gctgggtgcg cagctcgcga tcgagcgccg
    12841 agaacggctc gtcgagcagc acgagctccg gttggcgagc gagtgcacgc gcgagcgcga
    12901 cgcgctgtgc ttcgccgccg gagaacgttc gcgggcggcg catcgcgagg tggagcacgc
    12961 cgagcttctg cagcagcgtc ttcgcccgct cggcgcgctg ctcgcgcggc acgtgcgcca
    13021 ttccgtacgt cacgttgtgg agcgcgctca tgtgcgggaa cagcgcgagg ccttggaaca
    13081 cgtacgagag gcgacggcga tggatcggaa cgtcgatgcc cttcgcgcga tcgagccaga
    13141 cctcgttgcc gactttgacg ctgcccgcgt cggggacggt gagccctgcg agcacgccga
    13201 ggatcgtcga cttgcccgag cccgacggcc ccatgatgca cgtgatgccc gcaggacacg
    13261 cgagatcgac gtcgagcttc cactccgccg agcgcgacag ctgaatcttc gcctggaggt
    13321 cagccacggt acgggccgat ccggcgcgtc aggcgatttg cgatgtagag gagcgcgacg
    13381 ccgacgatcg tcgtcgcgat cgccatcatc cgcgcctcgt gctcgcgatt cgcaaccatc
    13441 gcgtccatca cgtagatcga ggccgtcggc gtgccgttga tgcgcgagcc cgcgaccatc
    13501 tgggtgatgc cgtagtcgcc gagtgcgcgc gcgaagccca gcatcgctcc tgcgatgagg
    13561 cccggtgcgg cgagcggcag cgtgacggtc aacgtgaccc gaccggggga ggcgccgagc
    13621 gttcgcgccg ccgagatcaa attcgggtcg accgactcga gaccgacgcg gatcgagcgc
    13681 acgacgagcg gcagcgagcc gacggctgcg gcgatgaccg cgcccgtgaa cgtgaagacg
    13741 atcgtcgtgc ccgtcatacg ctcccacgcg cgaccgacgg cagactccgt gccgagcgcg
    13801 acgagcaagt agtagccgag caccgtcggt ggcaggacga gcggcgccga gacgatcgcg
    13861 tcgaacaggt ttgctgccgg tagccgccgc cacgtgagca gcagcgccag gccggtgccc
    13921 acgacgagtg tcagcagcgt cgcgagcgtc gcgaccttca gcgacaggat cagcggatcc
    13981 caggtcacga gcgttagtcc ggtggaagcc gcaccacgaa gaccgcgccg agctcgccgc
    14041 gttcctcgac gtgaatggtg ccgccgtgag cttcgactgc gagcttgcag aagtagaggc
    14101 caaggccgcg gttggcgcgc gccgatgcgc gacgacgttc gacttgaaag tagcggccga
    14161 agatcttgtc gcgctccgcg ggcgggaccg gcgggccgct gttgccgatc gcgatctcga
    14221 cgccttggtc atcccgcttc acggcgaccg agcagcggcc acctcgcggc gcgtagcgcg
    14281 cggcgttgtc gagcatgttc tcgatgacgc gcgagaacag atcgcgatcg atctggacgg
    14341 tcatctccgg atcgacgtcg acgtcgacgc gcaattggcg cacttcgaag tgagcgcggt
    14401 tgcccgcacg cgcgcgctcg atgagctcgg tgatcttcac cggcgatttc atcgcgcgaa
    14461 ggatgccgtc ttcgagctcg tcgacatcga ggatcgtcgc gaccagcgag agcgccttgc
    14521 cgagcagcag attggcgtcg tcgatgtact gcttcacgac gtgcgaggcg tcgtcgccga
    14581 cttcttcgcg caacaggtgc aagtagcctt ggacggccga cagtgggctg cgcaggtcgt
    14641 gcacgacgag cgctgcgagc acccgcttct tctcgcggag ctctgcattc ggatcgactg
    14701 tcatcgagtc ccgtcgccta gccgaccacg cggttgcggc cgttttcctt cgcgctgaac
    14761 agatgcgtgt cggcctgctt gatgaactcg agcgcgtctt cgatctcacc gcgcagcgac
    14821 gcgacgccga tcgaaatcgt caccggaatg acggcatcct cgaacttgaa ctcggcaacc
    14881 tcgacgagct tccggatctt ctcggagaac tgcatcgcgt tgtagtgatc gatctcgggc
    14941 agcacgatcg cgaactcttc gccgccgtag cgagcgagca cgtcttcgcg gcggatgcgc
    15001 gtcttgatca cggacgcgag gtgcttgagc acgtggtcac cggcgaggtg gccgaagcta
    15061 tcgttgacgc ctttgaagcg atcgatatcg aacatgatga gcgagaggtc gcggcggtag
    15121 cgcagcgctc tcccgatctc gcgctcgagc gtctcgacga agtagcgacg gttgaagacc
    15181 tgggtcagac cgtcgaccgt cgtgaggcga taaatctctt cgtggtacgc gttctcgatg
    15241 ttgttgccgc tgaggaactt gaagatgcag cggccgactt tgatgaagtc gccatcgcga
    15301 aggacgtact cgtcgatcat ctcgtcgttg acgtaggtgc cgttcgtcga ccccatgtcg
    15361 cgaagcatga tcgcgttacc ggtgttgatg atcttgcagt ggtttcgcga gaccgcttct
    15421 tgatcgatct ggatgtcggc cttgctcgat cggccaatga tgatctgggg gcggttgagg
    15481 ttgaacttct tgcccagctc gaggccgtaa atcacgacca ggcatgcctc tttggcgacc
    15541 ggtcgatcgc tgatcttgga gatcgcggtg acgacggtct tgttacggcc ggtatcgctc
    15601 acccaccgga gatcctatca ggatttccga caggtcacct atttgtgtcg gcgcaatcga
    15661 ggcggtcgtc gggggcgcgc ttacactgga gcacgtcctc gatccgatag ccctcgcgca
    15721 cgtagcggta ggactgcagc agcatgcgtc cgccgctctg ctgccagtag gagcggacgt
    15781 cggtcatgtt gtcctcgcca cgcgacgcga gcggctcgag gttagctccg gcaaacgtcc
    15841 cggcgtaacg cgcgcagtcg cttgggtcac gcggctgacg aaacacgttc cagtagcaat
    15901 tgccgtcact ggtgcactgc gcgcggtcga cgacgatgat ctccgcgcga ccgtcgttat
    15961 cgagatcgcg atcggcgtgg tcgaccttcg gcgagttgct catgacgccg tcgcgcccgg
    16021 gctccgcaca actcccaggt tgcacactcg cggtcggcat acggatcgtc gtcggcgtag
    16081 acgccggttt tttcgtgcac gcggtaagtc ctaccgtcag gaccaaccat cggacggaag
    16141 tccgcatcgg ggaggaccct actgcaaagt tcttgtgctt gcgagcagat gcggctacat
    16201 ccactagagc gatgcgacgc gtggtcctcc tcgccggtgg tgtgctgctc gccggagcca
    16261 gcgtgttcgt caacgcgggt cctgatcaca agcccaagaa gccaacgccc aaggcgagca
    16321 ttcagggtga gtcgctcgtc gccgcgccgt caggtccgat cacgacgcca tcgcagctca
    16381 gcgtcgacac caagacgtcg gacgtgctca gcgatctgca gctcgacaag ctgacgctcg
    16441 aggacggcca ctacgtcgcg ccgctcaaag atggtcgccg cgccgtgctc acgctcgatc
    16501 ccgatctgca gaagctcgcc gagaagctgc tcgatcaatc gcgtgcacca cgcggcgcga
    16561 tcgtcgcgat gtctcccgat ggccgcatcc tcgctctcgc ggggcgcagg accgaagagc
    16621 ccaagggcgg caagaccggc acgttcgact acaagctcgc gaccgacgtg tgggcgcccg
    16681 cggcgagcgt gttcaagctc gtcaccgcga cgtcgctcgt cgcgaacggc tacgacccga
    16741 gcgagaaggt ctgctatcac ggcggcgttc gctcggtgat ggagagcaat ctctcggact
    16801 ccaagagcga caacaactgc gagacgctcg cctacggcgt cgcgcactcg aacaacgcga
    16861 tcctcggcaa gctcgccttc caaaagctcg agccctcgct gctcgatcgc accgcgcgtg
    16921 acctcggctg ggcgagcaag caagagctcg gcgtcgaagc cacgtgcggc gagctcgcgc
    16981 tccccgagaa gaaagacctc gagttcgcaa aagccgccgc cggtttccaa ggctcgcggc
    17041 tgtccgtgct cggcggcgcg atgctcgtgt cgacgttcgc cggcaaaggc gagcagccgc
    17101 agccgcgcat catcgattcg atcgccggca agcccgtcga tccgggcaag ccacatcgcg
    17161 tgatcgacga gaaggtcgcc gaggccgtca cgaagatgat ggtcggcacg tgcgcgtcag
    17221 gcagcgcagc gaagagcttc cgccgtagcg ataacaacgt cgcgggcaag acgggaacgc
    17281 tcacgacgaa caagccgttc tacatggagc acagctggtt cgtcggcttc gcgcccgcgg
    17341 acaagcccga gatcatcgtc tcggtcctgc tcggcaatcc cgagtcctgg cacttgcgcg
    17401 gccatgaagc ggccaagcgc atgatcgacc gtgcactctc tgccaaatcg agtgcgccag
    17461 atctcagcga cgagccttcg aaaagcgcag caaaacgccc gaagtgatag gatctcaggc
    17521 gtcgtcctgg gaggaactat gactaggcta agactaggtc tcgtgctcgc gtcgtcgctg
    17581 cttgcgacgc atgcgttcgc agaaacccag gccgaaattg cggcgcggga gaacgaagaa
    17641 ggcaagcagc tcatgttcga gaacaagtac tcggaagcga gtgcgaagtt catgagcgcg
    17701 cacagccgcg tccccgagcc gaagtatttc ttcaatcact gcacgtcgct gttctgggaa
    17761 ggcaagttcg gcgagtcgct caccgcgtgc aacgcggccg acaagaacgc cgacgacaag
    17821 ctcaaggcaa agatcgcggc gttcgaagac aagatcaaag ccgaagcaca gaagcaaggc
    17881 gtcgtgctgc aaccagtcgg cggcggcgca ggtcctggcg agacgcctcc ggtcggcacc
    17941 gatccgaaca acccgaacgg caacccgccg ggtcctggtc caacgggccc gagtggacag
    18001 cctcctccga gctacggcgg cgttggtcgc gcgccatcga tgggcctctt cagccaatcg
    18061 aagcccgaga acaaatacgc gtggagcctc ggcatcgaca tcttcggtgg cggtgggcgc
    18121 atcggtcagc ccggctactt cggccacgcg gccggcggct tccggctcaa gagcgactac
    18181 ctgctgaacc cgaagatgcg cctcggcacg cagggctact tccagatctc gaactacggc
    18241 aacggcgatg agatgggcgc gcaaggcgcg tactcgctcg gcatcttcga tctcggcatc
    18301 gcgggctaca aacacatctg cggcccgcac tcgcgcttct gtctcacgcc gctcgttggt
    18361 gttcagctcg cgctgctctc gccggcgaat cagacggact cgacgggcga cacggtcttc
    18421 aactacgcat cgctcggcgc gcgtctcgag atggctgcgt cgctcatgct gggcagccgc
    18481 taccagcacg tcatctccgg catcatcggt ctcaatgcct acacacaggc attctccgag
    18541 ccggcagatt gctcgcccgg cttcgactgc aaagaagact ggggcctcga taaaggcggc
    18601 gccgcgtttt acctgggtgc cggatacaca tacaggttct cgacgccgtt cggcagcgct
    18661 ccgttcgtga cgctcgaata gccttccgcc cgtcgagatc gggtatggtt cggccacaat
    18721 ggctggaccc gagaagctga gtgcgaagct ctccctccct ggagggaagg aagcgacgct
    18781 tcccgtcatc gtcggcacgg aagacgagca cgccgtcgat attcgttcgc tgcgccaaga
    18841 caccggtttc atcacgctcg atagcggcta catgaacacc ggctcgacga cgagcgcgat
    18901 cacgtacctc gacggcgaac aaggcatcct gcgctatcgc gggtattcga tcgaagacct
    18961 cgccgagaag agcgacttcg tcgagacgag ctacttgctc atccacggcg agttgccgac
    19021 gaagcagcag cgcgagacgt tctcgaagaa cctgacgcgc cacagcctca ttcacgagga
    19081 catgcaccac ttcttcgccg gctacccgcc gaccagccac ccgatggccg tgctgtcgtc
    19141 gatggtgacg agcctgtcgg cgtactaccc ggactcgctc gaccgcaaca gcgcgatcga
    19201 cctccacatc acgcgcatcc tctcgaaggt ccgcacgatc gcggcgttcg cgtacaagaa
    19261 gtcgatcggt cagccgttga tctatccgaa gaactcgctg cgctactgcg cgaacttcct
    19321 ccacatgatg ttcgcggttc ccagcgagga gtacgaggtc tcgccgatcc tcgagaaggc
    19381 cgtcaaccag ctgctcatcc tgcacgccga tcacgagcag aactgctcga cgtcgacggt
    19441 gcgcatggtc ggcagctcgg gcgcgagcat ctacgccgcg atcgcgtccg gcatcctcgc
    19501 gctgtgggga ccgctccatg gtggc
//
LOCUS       DATLBZ010000303        16579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_338090, whole genome shotgun sequence.
ACCESSION   DATLBZ010000303 DATLBZ010000000
VERSION     DATLBZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16579
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338090"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1101)
                     /locus_tag="VMZ53_15550"
     CDS             complement(109..1101)
                     /locus_tag="VMZ53_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29920.1"
                     /translation="MKKTGLVLAVLLGTAPSAVAQPGTPTPSDPPPDPQPAQPTSPGP
                     TSTLDSGVGMPAMTTTPGADKPAPPRAQKKKEPGRGDFDAGGQVRLPNGPDENSKYAA
                     FNWIAFDMKGRYFLLDQVTLNGNIPLAVKKPDMLMNGADPRLFGGMTVRLDAIFTAPK
                     MPMVPSKGESKIGLSLTGAYMREGAMLLTDKDFPLFVGDMKPGFAGALVTRVALASVV
                     DFSLMPAWIYQSGTTDSHKAVQIPMALIIKIGDLIKLSTDLNIATGDDYSMRGKNGGR
                     LGAGVALDLKIGPILGHVGTGVASLLTGDPMTTKAVYPSISDSVYFEINAKYVK"
     gene            complement(1171..2913)
                     /locus_tag="VMZ53_15555"
     CDS             complement(1171..2913)
                     /locus_tag="VMZ53_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29921.1"
                     /translation="MRTFLPVLAGAVLCAACGSSVSPTEAQKVSFERWSPPQATGHDV
                     LLGSRGDVVVMAHRYSLDAGATWKPLDARLGEPTRVSIVGNTVALFANGLVRWNLGDG
                     SITSAASVPSFATDRTWRVDPQSARFMAFDAVENAIAIESSANTWTTSKLPQPSSTET
                     RPYIRDIESNGSVLLSASVWGVHRSTDGGSTWQLVVGSTPNAGRDLLVLRDGTFALVG
                     GTTTYAFDAAGQAAGTLPKLVLPNEATVCEDGTIVTGTKLTRDLGATWETLIAQSDLQ
                     MSVQRASCGSTGKYWVLVISDAWGYRLVRFDAVGTPGAAAGNWEAVGDQAWTNGGPPI
                     LRTDDGTFLVAGLALAPNGTTWTLQEIPAKTWAAGTTLFGVAKQKFFASYDGGVTWSA
                     AAANGLMAADPEAFAKSPDGALYVAQFQGGSADGQDLWRSSVWKTQDQGASWTMAYDA
                     MATRVTGDDKVMGEVHRFVGITMTGDWIATDAVSRDAGNTWQKTTVLGDRGLAHLTRQ
                     GSLVTGGADEKVWRVYDDGGLGELRATYQIVVEGNAVPASQLRSVAFDNEGYAYIARG
                     APQVQIWRSDRPLE"
     gene            complement(3001..5307)
                     /locus_tag="VMZ53_15560"
     CDS             complement(3001..5307)
                     /locus_tag="VMZ53_15560"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HUS29922.1"
                     /translation="MTRKTLFALVLAAACSPKAPSPSETGPQERSPVAAGQSSAQPAA
                     PLPAPDPREKALATTVVELFEQEHLLRKKIDDGISKEAFKNYLEALDPGKMFLLATDR
                     DALGKYADKIDDELHSGSLDLAHDGQKTFTARVEVVQKVVDELLAKPFDLTNEEYVEL
                     DAEKLDLAKSEDELKERWRQRLELEVLERIASMDARLKADAEKKAKKDAPKGKNDKAG
                     KGDKKQPDASPVDEADDEEDKSAPPIAQIPATAEGREAKAREDLLKTYSSRFARMKNP
                     GQLDAASDLINAVASTLDPHTTYLPPAEKANFDIRMSGSLEGIGAVLREKEHLIEIVE
                     IVPGGAAYRQGGLVVGDLIESVQQEGKDPVDVFDMRIDDVVSMIRGAKGTVVRLRVQK
                     ASGQEQTIAITRDVVVIEESYAKGAILQPKGSKTTFGYIHLPSFYGGKGSPRSADDDI
                     KKLLEDMRDKKVAGVILDIRSNGGGLLTGSINISDHLIDKGPVVQVKDSDGDKEVLSG
                     RVKGEAYDGPVIVLVDRFSASASEILAGALQDYGRAVIVGAGGTHGKGSVQTLADLDR
                     ATGGQIELGVLKITIQQFYRPDGDSVQLQGVTPDVMLPDPTAYIDSGEGELKHAIPAS
                     KIEAAPFAKWPMQFNVKDLAAASSSRVAKHPLLAKIASATAVLRSRKNDTKVPLQQTA
                     WQKRRDEQKAQLDAASPDLKKVAPVFTVKPLDDSPAPANAPAPGGSAGAKDDKAAKWR
                     DNLARDPWVEETLNIMTDMTQQKVAAKK"
     gene            5408..6367
                     /locus_tag="VMZ53_15565"
     CDS             5408..6367
                     /locus_tag="VMZ53_15565"
                     /inference="COORDINATES: protein motif:HMM:NF015716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HUS29923.1"
                     /translation="MDPSIAVQDVIEPMPEEAGPAAVLRGTARGLEIVVDATAATSAI
                     TAAVTKRLEEAPGFFRGSDVRVRVEEGPLAPGCLARLDDLALQYELRIVEVTAQKRRL
                     DTANDADAVPVPESLAAGSAPSPADFGDESPTNAGAKLISVPPAAPEIQPAALIDIED
                     VVVANAPELSGSPETFDEPTQTAIAAPLTLASLPDAELATHTPGTRIVIGPVRSGVIL
                     DHTGHVVVFGDVNPGAEVRAAGNIVVLGRLRGTAHAGIGQDVGFILALRLEPQQLRIG
                     RQVARAADSETPGTEPELAYGTGDKIIVERYQGKLPRNLATSI"
     gene            6381..7175
                     /gene="minD"
                     /locus_tag="VMZ53_15570"
     CDS             6381..7175
                     /gene="minD"
                     /locus_tag="VMZ53_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HUS29924.1"
                     /translation="MGRAIVITSGKGGVGKTTTTANLGAALAQLGHTVVLIDADIGLR
                     NLDVVMGLENRIVYHVVDAIKGKCTVQKALIKDRRMENLWLLPASQTDDKDSVTPDDM
                     RSLVFELKTTYDFVLIDCPAGIEQGFKNAIAGADEAIVVATPEVSSIRDADRVVGLLA
                     AADVPARLIVNRISPHLVKRGDMLSQSDVIEILALDLLGAVPLDDQIIATTNKGVPAV
                     LEGKSAAAKELISIAKRLSGMEADERESVGFFTRIGRALGLSATPA"
     gene            7172..7444
                     /gene="minE"
                     /locus_tag="VMZ53_15575"
     CDS             7172..7444
                     /gene="minE"
                     /locus_tag="VMZ53_15575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HUS29925.1"
                     /translation="MRSHLNMWNSVKTFFTGKSSRDVAKGRLHLVLAHDRTGLEGGKL
                     QEMRAEIAAVLAKYVSIDADAVEIHIETHSRETQLQISSSIAPRNA"
     gene            complement(7445..7888)
                     /locus_tag="VMZ53_15580"
     CDS             complement(7445..7888)
                     /locus_tag="VMZ53_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29926.1"
                     /translation="MRWVFVCVVMLGCWSNSKSSTSTTNAAGGGDGSAAASDTDTATA
                     TATTTTAPLPAGCKRMRQAYVSRCSGVPPGPREPNSSATTVCDECLAASDCTAQPGGQ
                     CEQRGDNMCSGARRAVCLYPDPACGGGICAERVIPPPPSAPPSSR"
     gene            complement(7907..8485)
                     /locus_tag="VMZ53_15585"
     CDS             complement(7907..8485)
                     /locus_tag="VMZ53_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUS29927.1"
                     /translation="MFSDAVKRIQAERGSRTAYAKVEAHGGFNVDVTDELRDFVAMID
                     TAFLATADAEGQPYVQHRGGPKGFIRALDANTLAFADLAGNRQYISTGNLTENAKVCL
                     FLIDYAHRQRVKVWGTARMVAATPELLERLAAPKGARAEQVCLITVTRWDVNCPKHIP
                     IKLDAQDVAAAIEKLQARISELEAENAALQSR"
     gene            8583..9797
                     /locus_tag="VMZ53_15590"
     CDS             8583..9797
                     /locus_tag="VMZ53_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29928.1"
                     /translation="MRRWLVSVSLVAMACGGHDHNGGGDDDQQTCPCDARMPDSPPDS
                     PTDAPPDAPPVMAAMQVGSTGGTLTTTDGVKLVIPAGALATMTNITITQLTNNLPNGV
                     VSNVYELGPDGTQFATPVELTIPYDVAKLGSQPESDLVVASNNQGQARGTGWSVVNAT
                     DHTVSALITHFSSWAVYPAVGGCNLNYGCMKQCSGVNVPELCCNSLRSTCRSKLTTSF
                     PDYVNCYTACTGANQVANFGNSECMSACCTDAGWTHLQQGACYKASGATQSQAQGILD
                     CAAACPATGDRGTFCGPISFSACEWNVNMSPNMGDECDGPNSGGVNSQVLATGLGTVG
                     DQIWGNPQVIHVVSGSYGSASLTLGTTCASAVSASGTMSGTWDGAQFSGTWTFGSSTS
                     GTFTVAPRWPQN"
     gene            10008..10619
                     /locus_tag="VMZ53_15595"
     CDS             10008..10619
                     /locus_tag="VMZ53_15595"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HUS29929.1"
                     /translation="MAIQRRARIVDRDGTFGWLRRIKADDPDRPYAVVTLGYGAVVEV
                     PFELLEHHDDGGYALGGRWRDFSIKSEEVASIPVIEERVRVDVRPAPARQLRVKRRVV
                     SERQTVETPVWHEHIEVERVPVDGSFVDRAPKPRREGDVLIIPCVEEVVIVEKRLRVR
                     EELRVRVVRERKLHRQTVDVRRHEIEIDESYEEPEPTPLVKGE"
     gene            10623..11414
                     /locus_tag="VMZ53_15600"
     CDS             10623..11414
                     /locus_tag="VMZ53_15600"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HUS29930.1"
                     /translation="MKTIIAAFSDQTAAQNAINFLNSKGVTDAQLMSSNAGGDMMSTF
                     RTLNVPEERAQIYSEVMRRGALLIVAHVEDDARSLAHDLDKMGSLDLDQAASRWRESG
                     WTGYDASAGPFDATASATERSALVSESGRRRDSAGARDLDVIEERVTVGKREVSKGGV
                     RVRTSISERPVEEQVQLTEERIDIQRERVDEAISPNAADAGLTEDEFVVTARGEEAVV
                     GKEARIVERVHVGKQADTRTETIRETERRRDVEVEPIEETRDRRR"
     gene            complement(11423..12313)
                     /locus_tag="VMZ53_15605"
     CDS             complement(11423..12313)
                     /locus_tag="VMZ53_15605"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS29931.1"
                     /translation="MQALREVTVTDARHHSPGPCLVGEPIAPGSTLAGRYRIKRFVAA
                     GGMGEVYEADDQLLGDPVAVKLLRADLMAKPGAQARFADEIRLARRVTHANVCRVFDV
                     GTDGDRVFYTMELHAGETLKAHLATRGCLSIGEIQPIVRQLLAGIGAAHAADVIHADL
                     KPSNVLIGANNRVVVTDFGLALPCCATLGCACDMPHLIGTPAYMAPEQVTGGTILEGT
                     DLFSFGVIVYELLTGDLPWHGATPLELAHARLTGEPPSMRARRAEIDATWDDVVRNCL
                     RVDITQRPKSAAAIATALSL"
     gene            complement(12379..12798)
                     /locus_tag="VMZ53_15610"
     CDS             complement(12379..12798)
                     /locus_tag="VMZ53_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29932.1"
                     /translation="MGFQRCLYAVLLSGCATTGVLGADREPAVPHAKVVLSPLPGDDT
                     VRLVPQAIEPMLPSADRIARVVESRLGPQASVDIHYCVSPAGKVVEAKLERGSSLEQF
                     DQAVMADIVDWQFEASPGPDSLRTCDSATIVYRPHRI"
     gene            complement(12921..13886)
                     /locus_tag="VMZ53_15615"
     CDS             complement(12921..13886)
                     /locus_tag="VMZ53_15615"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HUS29933.1"
                     /translation="MGLLLRASAALTVLVVACSPGGGGMGTGDDAPDAAVTTPMPDAA
                     TTTAANCDGKTAQPLDATWNITVGSAMRVARVHVPASYSPQSRTPLVLDIHGRSGWGS
                     QQAALAHAIAKSDAEGFIVIHPEGTGSPTSWNSGGGCCDPAAANNVDDVGFMRAILDK
                     AEAQLCVDPARIYAMGLSNGGYLAHRIACDLGDRVAAIGAVAGLLQGNCTPARPVPVW
                     MVHGTSDTLVDYSWVPATVNFWDATNGCSTESTTYTHGDASCVTHAGCTANASLVLCT
                     IQGGGHQWPGGDALPFLGKKSDDIIATDALWDFFVAHPLPSAAMQ"
     gene            complement(13853..14782)
                     /locus_tag="VMZ53_15620"
     CDS             complement(13853..14782)
                     /locus_tag="VMZ53_15620"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HUS29934.1"
                     /translation="MRILLAVVVVGCSGSGSQQQADVDAATTMMADSSTGGMSSMTCE
                     GKTTQPLDATWNIMAGGAMRVARVHVPASYDPQKATPVVFDVHGRTQNASGEMSLSNS
                     KAKSDAEGFIVVFPESGTSPTAWNSGTCCDPASTNKIDDTAFMTKLLDEVEAKLCVDT
                     KRVYMMGMSNGAYESQRIACELADRFAAVGPVAGLLLFQGCAPSRPVPIMMVNGTADT
                     LSQYQYVPQGVDFWKAKNKCTTMQQSYQKGDVTCVTHGGCTGGADVVLCTVQDGGHQW
                     PGGGSTFPFLGTKSDNLDTTSALWDFFSAHPLP"
     gene            complement(15246..16127)
                     /locus_tag="VMZ53_15625"
     CDS             complement(15246..16127)
                     /locus_tag="VMZ53_15625"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HUS29935.1"
                     /translation="MLHQENTLAGFRRAIQLGVPAIELDVQLTKDRKAVVLHDGHLGR
                     LTGDSRYVWQVPWDEVSKLRIKRELPMGVDVHGQRTIIRYESEERIPLLAEVLAEVAG
                     KVAINVELKINMRGWWSTEVARVTADVVAAANAEDSVILTSFDPRKLRAATAAHPKLA
                     TGFCFDDSMLDWSAPFLDRLPHVRAKLSLPAEGHIRTNARRLLNRILETNLVGKLLGT
                     RLVGAEHTIVGSQTVKRLHDLGIAIGTHTLFPLGSTTGKPISPSAMTAAEVERLVSLE
                     VDWIESDDPERLLALVG"
     gene            16014..>16579
                     /locus_tag="VMZ53_15630"
     CDS             16014..>16579
                     /locus_tag="VMZ53_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HUS29936.1"
                     /translation="MQHYRFAIFRELNVELDRRNAELDGAPKSSERIFLMQHRNAAVS
                     DHHRLVLAGQERVRHRRAHVFPLNLTLMLLDRRRFLVGSAASTLLLACQKSAKTATLR
                     EVDHVVAAQASRDGAGVALRRSIGSGVLPNLDPFLLLDEMHSSDPADYVAGFPQHPHR
                     GFETVSYVLRGGFQHRDSVGNSGLIADGG"
BASE COUNT         2735 a         5519 c         5453 g         2872 t
ORIGIN      
        1 attttcgcgg gtgtcgatgg tctggacgag ctgcgcatct tgctcggtgc cgcatgccgg
       61 gcagcgcacg cgtgtcgttc cgcggatgac catcccggag gtgtagcgct acttgacgta
      121 cttcgcgttg atttcgaagt acaccgagtc gctgatcgac ggatacacgg ccttcgtcgt
      181 catcgggtct ccggtgagga ggctcgcgac gccggtgccg acgtgaccga ggatcgggcc
      241 gatcttgaga tcgagcgcta cgcccgcgcc gaggcgaccg ccgttcttgc ctcgcatcga
      301 gtagtcatcg cccgtcgcga tgttgagatc ggtgctgagc ttgatcaggt cgccgatctt
      361 gatgatcagc gccatcggta tctgcacggc cttgtgtgaa tctgtcgtgc ccgactgata
      421 gatccatgcg ggcatcagcg agaagtcgac gacgctcgca agcgcgaccc gcgtgacgag
      481 cgcgcccgcg aagcccggct tcatgtcgcc gacgaacagc gggaagtcct tgtcggtgag
      541 cagcatcgcg ccctcgcgca tgtacgcgcc ggtcagcgac aagccaatct tgctctcacc
      601 cttcgagggc accatcggca tcttcggtgc tgtgaagatg gcgtcgagac gcacggtcat
      661 gccgccgaac agacgcgggt cggcgccgtt catcagcatg tcgggctttt tcaccgcgag
      721 cgggatgttg ccgttgagcg tcacctgatc gagcaggaag tagcggccct tcatgtcgaa
      781 cgcgatccag ttaaaggcgg cgtacttgct gttctcgtca ggaccgttgg gcaagcgcac
      841 ctgaccgccg gcgtcgaagt cgccgcggcc tggctccttc ttcttctgcg cccgcggcgg
      901 tgccggcttg tccgcgccgg gcgtcgtcgt catcgcgggc atgccgacac ccgagtcgag
      961 cgtcgacgtc ggaccaggcg atgttggttg tgccggctgt ggatcaggcg gcggatcaga
     1021 cggggttggt gtaccgggtt gcgccaccgc gctcggcgct gtccccaaca gcaccgccag
     1081 tacgagacca gtttttttca ttgttagctg ccagtagacg gtggaggtcc gttagacctc
     1141 cagcctcatg accgttaggc agcagtgatt ctattcaagt gggcgatcgc tgcgccagat
     1201 ttggacctgc ggcgcaccgc gcgcgatgta ggcatagccc tcgttgtcga acgcgaccga
     1261 gcggagctgc gaggctggaa cggcgttgcc ctcgacgacg atctggtacg tcgcgcggag
     1321 ctcgccgagg ccgccgtcgt cgtagacgcg ccacaccttc tcgtcggcgc cgccggtgac
     1381 gaggctgcct tggcgggtga gatgcgcaag gccgcgatcg ccgaggaccg tggtcttttg
     1441 ccaggtgttg cccgcatcgc gcgacaccgc gtcggtcgcg atccagtcgc cggtcatggt
     1501 gatgccgacg aagcgatgca cctcgcccat caccttgtca tcgccggtca cgcgcgtcgc
     1561 catcgcgtcg tacgccatcg tccaggacgc accctggtcc tgcgtcttcc acacgctgct
     1621 gcgccagaga tcctgaccgt cggcgctgcc gccctggaac tgcgcgacgt acagcgcgcc
     1681 atcaggactc ttcgcgaatg cttcggggtc ggccgccatc agcccgttcg ccgctgctgc
     1741 cgaccacgtg acaccgccgt cgtaactcgc gaagaatttc tgcttcgcga cgccgaacag
     1801 tgtcgtgccc gcggcccacg tcttggccgg aatctcctgg agcgtccacg tcgttccgtt
     1861 gggcgcgagc gcgaggcctg cgacgaggaa cgtgccgtcg tcggtgcgca agatcggcgg
     1921 accgccgttc gtccatgctt gatcgccgac agcttcccag ttgcccgccg ccgcgccggg
     1981 cgtgccgacc gcgtcgaacc ggacaagccg gtagccccac gcgtcgctga tgacgaggac
     2041 ccagtacttg ccggtgctgc cgcagctcgc gcgctgcacg ctcatctgca gatcgctctg
     2101 cgcgatcagc gtctcccacg tcgcgccgag gtcacgcgtc agcttcgtgc cggtgacgat
     2161 cgtgccgtcc tcgcagacgg tcgcttcgtt cgggagcacg agcttcggta gcgtgcccgc
     2221 ggcctggccc gcggcatcga acgcatacgt tgttgtcccg ccgacgagag cgaacgtgcc
     2281 gtcgcgcagc acgagcaggt cgcggcctgc attcggcgtc gagccgacga cgagctgcca
     2341 cgtcgatccg ccatcggtcg aacgatgcac gccccacacg ctcgccgaga gcaggacgct
     2401 cccgttgctc tcgatatcgc ggatgtatgg ccgcgtctcc gtcgacgaag gttgcggcag
     2461 cttgctcgtc gtccacgtgt tcgcgctcga ctcgatcgcg attgcattct cgaccgcgtc
     2521 gaacgccatg aagcgcgccg actgcggatc gacacgccac gtgcgatcgg tggcgaagct
     2581 cggtacggac gccgccgacg tgatcgatcc atcgccgagg ttccagcgca cgagcccgtt
     2641 cgcgaagagc gcgacggtgt tgccgacgat cgagacgcgc gtcggctcgc cgagcctggc
     2701 gtcgagcggc ttccacgtcg cgccggcgtc gagcgagtag cgatgcgcca tcacgaccac
     2761 atcaccgcgc gatccgagca acacgtcgtg acccgtcgcc tgtggtggcg accaccgctc
     2821 gaacgagacc ttctgcgcct cggtcggcga aacactgctg ccgcacgcag cacaaagcac
     2881 agcgccagcg aggacgggga ggaaggtccg catgatgaag gatggacctc acctgccgcc
     2941 ggcgcttgcc gacctgacgt gtccctcacc agtgcgccga tgcgcggtgt gccgtggcgc
     3001 ctacttcttc gcggcgacct tctgctgggt catgtcggtc atgatgttga gcgtctcctc
     3061 gacccacgga tcgcgagcga gattgtcacg ccacttcgcg gccttgtcgt ccttcgcacc
     3121 ggcggagccg cccggcgcag gcgcgttcgc gggagccggc gaatcatcca gcggcttgac
     3181 cgtgaagacg ggcgcgacct tcttgagatc cggagacgcg gcgtcgagct gcgccttctg
     3241 ctcatcgcgg cgcttctgcc atgcggtctg ctgcagcggg accttcgtat cgttcttgcg
     3301 cgagcgcagc accgcggtcg ccgacgcgat cttcgcgagc agcggatgct tcgcgacacg
     3361 cgacgagctc gctgccgcca gatccttcac gttgaactgc atcggccact tcgcgaacgg
     3421 cgcagcctcg atcttcgatg ccgggatggc gtgcttcagc tcgccttcgc ccgagtcgat
     3481 gtacgcggtt ggatcgggca gcatcacgtc gggggtcacg ccctggagct gcaccgagtc
     3541 gccgtcgggg cgatagaact gctggatcgt gatcttgagg acgccgagct cgatctgacc
     3601 accggtggcg cgatcgagat cagcgagcgt ctggaccgag cctttgccgt gtgtgccgcc
     3661 ggcaccgacg atgacggcgc ggccgtaatc ctggagcgcg ccggcgagaa tctcggacgc
     3721 tgatgcgctg aagcgatcga caagcacgat cacgggaccg tcatacgcct cgcccttcac
     3781 gcggccagag aggacttcct tgtcgccgtc actgtccttg acctgaacga cggggccctt
     3841 gtcgatcaag tgatcgctga tgttgatcga gcccgtcagc agaccgccgc cgttgctgcg
     3901 gatatcgagg atgacgccgg cgaccttctt gtcgcgcatg tcctcgagca gcttcttgat
     3961 gtcgtcatcg gccgagcgcg ggctgccctt gccgccgtag aagctcggca agtggatgta
     4021 gccgaacgtc gtcttgctgc ccttgggctg caggatcgcg cccttcgcgt atgactcctc
     4081 gatcacgacg acatcgcgcg tgatcgcgat cgtctgctcc tgaccagacg ccttctgcac
     4141 gcgcaggcgc acgaccgtgc ccttcgcgcc gcgaatcatc gagacgacat cgtcgatgcg
     4201 catgtcgaac acgtcgacgg ggtccttgcc ctcttgctgg acgctctcga tgagatcgcc
     4261 gacgacgagg ccgccctggc gatacgcagc gccgcctgga acgatctcga cgatctcgat
     4321 caggtgctcc ttctcgcgca gcaccgcgcc gatgccttcg agcgagccgc tcatgcggat
     4381 gtcgaagttc gccttctcgg cgggcggcag atacgtcgtg tgcgggtcga gcgtcgaggc
     4441 gacggcgttg atcagatccg acgcggcatc gagctggccg ggattcttca tgcgcgcgaa
     4501 ccggctcgag tacgtcttca acagatcctc gcgcgccttt gcctcgcgac cttccgcggt
     4561 cgccgggatc tgcgcgatcg gcggcgcgct cttgtcttct tcatcgtcgg cctcgtcgac
     4621 aggagaagcg tcgggctgct tcttgtcgcc tttaccggcc ttgtcgttct tgcccttcgg
     4681 cgcgtccttc ttcgccttct tctccgcgtc cgccttgagg cgcgcgtcca tcgacgcgat
     4741 gcgctcgagc acctcgagct cgagacgctg gcgccagcgc tccttcagct cgtcttcact
     4801 cttcgcgagg tcgagcttct cggcatcgag ctcgacgtac tcctcgttcg tcagatcgaa
     4861 cggcttcgca agcagctcgt cgaccacctt ctgcacgacc tcgacgcgcg cggtgaacgt
     4921 cttctggccg tcatgcgcga ggtcgagaga acccgagtgg agctcgtcat cgatcttgtc
     4981 ggcgtacttg ccgagcgcgt cgcgatcggt cgcgagcagg aacatctttc cgggatcgag
     5041 cgcctcgaga tagttcttga acgcctcttt cgagatcccg tcatcgatct tcttgcgcag
     5101 gaggtgctcc tgctcgaaca gctcgaccac cgtggtcgcg agcgcctttt cgcgcggatc
     5161 gggggccggc aatggagctg ctggctgggc cgacgactgg ccggccgcta cgggcgaacg
     5221 ctcctgggga ccggtctcgc tgggggaggg cgccttgggg gagcaggcag ccgcgagaac
     5281 gagcgcgaac aaggttttac gcgtcacctc ttcaatgtag ccggtggcgg cggcgtttcc
     5341 acctgcgatg ccatgtcgaa aagttgcccg cgttcctggg gcggattacg gcttttgaag
     5401 ggcacttatg gatccatcta tcgccgttca ggacgtgatc gagccgatgc ccgaggaggc
     5461 cggtcctgcc gccgtcctgc gcggtaccgc tcggggcctc gaaatcgtgg tcgatgcgac
     5521 agctgcgaca tcggcgatta ccgccgccgt gaccaagcgc ctcgaggaag cccccggctt
     5581 tttccgtggc agcgatgtgc gggttcgcgt cgaagagggc ccgctcgcac ccggctgcct
     5641 cgcgcgcctc gacgatctcg cgcttcagta cgagctgcgc atcgtcgaag tgaccgccca
     5701 aaaacgccgc ctcgacaccg ccaacgacgc cgacgccgtt ccagtgcccg agagcctggc
     5761 cgccggctcc gcgccatcgc ccgccgactt cggcgacgaa tcgccaacga acgcaggcgc
     5821 caagctgatc tcggtcccgc ccgcggcgcc cgaaatccag cccgccgccc tgatcgacat
     5881 cgaggacgtc gtcgtcgcca acgctcccga gctcagcggc tcccccgaga cgttcgacga
     5941 gccgacgcaa acggcgatcg ccgcgccact gacgctcgcc tctctccccg acgccgagct
     6001 cgccacgcac acgccgggca cgcgcatcgt catcggcccc gtccgctcgg gcgtcatcct
     6061 cgatcacacc ggccacgtcg tcgtcttcgg cgacgtcaac ccgggcgctg aagttcgcgc
     6121 cgccggcaac atcgtcgtgc tcggtcgcct tcgtggcacc gcccacgccg gtatcggcca
     6181 agacgtcggc ttcattctcg cgctgcgcct cgagccgcag cagctgcgca tcggccgtca
     6241 ggtcgcgcgc gcggcagaca gcgaaacacc aggaaccgag cccgagctcg cgtacggcac
     6301 gggcgacaag atcatcgtcg agcgctacca gggcaagttg cctcgcaacc tcgccaccag
     6361 catctaaagg agttcacacc atgggtcgag ccatcgtcat cacatcaggc aagggaggcg
     6421 tcggtaagac gactaccacc gccaacctcg gcgccgctct cgcgcagctg gggcacaccg
     6481 tcgtgctgat cgatgcagac atcggcctgc gcaatctcga cgtcgtcatg ggcctcgaga
     6541 atcgcatcgt ctatcacgtc gtcgacgcga tcaaaggcaa gtgcaccgtc cagaaggcgc
     6601 tgatcaaaga ccgccgcatg gagaacctgt ggctcctccc ggcgtcgcag accgacgaca
     6661 aggactcggt cacgcccgac gacatgcgct cgctcgtgtt cgagctcaag acgacgtacg
     6721 acttcgtgct catcgactgc cccgcaggca tcgagcaagg gttcaagaac gcgatcgccg
     6781 gcgccgacga agcgatcgtg gtcgcgaccc ccgaggtctc ctccattcgc gatgccgacc
     6841 gcgtggtcgg tctgctcgcc gctgctgacg tcccagcgcg cctgatcgtc aaccgcatct
     6901 cgccgcacct cgtaaagcgt ggcgacatgc tctcgcagtc cgacgtgatc gagattctcg
     6961 cgctcgacct cctcggcgcc gtcccgctcg acgatcagat catcgcgacg acgaacaagg
     7021 gtgtcccggc cgtcctcgag ggcaagtcgg ctgccgccaa agagctcatc tcgatcgcga
     7081 agcgcctctc cggcatggaa gccgacgagc gcgagtcggt cggtttcttc acccgcattg
     7141 gccgcgcact cggcctctct gcaacgccgg cgtgaggagc catctcaaca tgtggaattc
     7201 cgtaaagacg ttcttcacag gcaagtcctc gcgcgacgtc gcgaagggcc gcctccacct
     7261 cgtgctcgcg cacgaccgca ccggtctcga aggcggcaag ctccaagaga tgcgcgccga
     7321 gatcgcggca gtcctcgcca agtacgtatc gatcgatgcc gatgccgtcg agattcacat
     7381 cgagacgcac agccgcgaaa cgcagctcca gatctcgtcg tcgatcgcgc cgcgcaacgc
     7441 ctaactagcg agacgacggc ggtgccgacg gcggcggagg aataacccgc tccgcgcaga
     7501 ttccaccgcc gcacgcagga tccggataga ggcacactgc tcgcctcgct ccgctgcaca
     7561 tattgtcgcc gcgctgctcg cactgcccgc ccggctgcgc cgtgcagtcg ctggccgcga
     7621 ggcactcgtc acacacggtc gtcgccgagc tgttcggctc gcgcggccca ggcggcactc
     7681 cgctgcagcg agagacgtac gcctggcgca tgcgcttgca gcccgcaggt agcggcgccg
     7741 tggtcgtggt cgcggtcgcc gtcgcggtgt cggtgtcgct cgccgctgcg gagccatcgc
     7801 cgccgccggc cgcgttcgtc gtcgaggtcg agctcttcga gttgctccag cagccgagca
     7861 tgacgacgca gacgaagacc cagcgcatcc gttgatcata cgctcgctag cgcgattgca
     7921 gcgctgcgtt ctcggcttcg agctcggaga tccgcgcttg cagcttctcg atcgccgccg
     7981 cgacatcttg cgcatcgagc tttatcggga tgtgcttcgg gcagttcacg tcccaccgtg
     8041 tgacggtgat caggcagacc tgctcggcgc gtgcgccttt cggagcggcg agcctctcga
     8101 gcagctccgg cgttgcggca accatccgcg cggtgcccca cactttgacg cgctgtcggt
     8161 gcgcgtagtc gatcagaaac aggcagacct tcgcgttttc ggtgaggttg cccgtcgaga
     8221 tgtactggcg gttgcccgcg agatcggcaa acgcgagcgt gttcgcgtcg agcgcccgga
     8281 tgaagccctt ggggccgccg cggtgttgga cgtacggctg gccttcggcg tcggcggtcg
     8341 cgaggaacgc ggtgtcgatc atcgcgacga aatcgcgcag ctcgtcggtg acgtcgacgt
     8401 tgaagccgcc gtgcgcttcg acctttgcgt aggcggtgcg agagccgcgt tctgcttgga
     8461 tgcgcttgac cgcgtctgaa aacatgggcg tttactacgc ggcagcgggg cggaggttac
     8521 agatgctgtg cgttagcccg taactacccg tgtggactcg aagacgcggg tacaatcgtg
     8581 cgatgcgcag gtggttggtg agtgtgtcgt tggtggctat ggcgtgcggc gggcacgatc
     8641 acaatggcgg tggcgacgac gatcagcaga cgtgtccatg cgacgcgcgc atgcctgact
     8701 cgccgccgga ttctccgacc gacgcaccgc ctgatgcgcc acccgtcatg gcagcgatgc
     8761 aagttggttc gacgggcggc acgctcacga cgaccgacgg cgtgaagctc gtgattcccg
     8821 cgggcgcgct agcgacgatg acgaacatca ccatcacgca gctgacgaac aacctcccga
     8881 acggcgtggt cagcaacgtg tacgagctcg ggcccgatgg gacgcagttt gcgacgcccg
     8941 tcgagctgac gattccatac gacgtcgcga agctcgggtc gcagccggaa agcgatctcg
     9001 tggtcgcgtc caacaaccag ggacaggcgc gcgggactgg gtggagcgtc gtgaacgcaa
     9061 cggaccacac ggtgtcggcg ctcatcacgc acttttcgtc atgggccgtg tatccagcgg
     9121 tcggtggctg caacctcaac tatggctgca tgaagcaatg ctcgggtgtc aacgtgccgg
     9181 agctctgttg caactctctg cgctcgacgt gccgcagcaa gctgacgacg tcgttcccgg
     9241 actacgtcaa ttgctacacc gcgtgcacgg gtgcgaacca ggtcgcgaac ttcggcaaca
     9301 gcgaatgcat gagcgcgtgc tgcaccgatg ctggctggac gcacttgcag cagggcgctt
     9361 gctacaaggc gagcggtgcg acgcaatcgc aggcgcaggg aatcctcgac tgcgcggcgg
     9421 cgtgtcccgc gacgggcgat cgcggaacgt tctgcgggcc gatctcgttc tcggcgtgcg
     9481 agtggaacgt gaacatgtcg ccgaacatgg gcgacgagtg cgatgggccg aatagcggcg
     9541 gcgtgaactc gcaggtgttg gcgacggggt tgggcaccgt gggcgaccag atctggggaa
     9601 acccgcaggt cattcacgtc gtcagtggca gctatggctc ggcgtcgctg acgctaggga
     9661 cgacgtgcgc gtcggcggtg agtgcgagcg gtacgatgag cggcacgtgg gacggcgcgc
     9721 agttctccgg cacgtggacg tttggatcgt cgacgagcgg cacgttcacc gtcgcgcctc
     9781 gctggccaca gaactaaacc gtcgagagaa cctcgcgagg tgacgtctgg ccgcgatgcg
     9841 gtctggtcgt tgcaccgcag ccgtgatgaa tccaacagga ggatcatcat ggccgaggtc
     9901 tatagtgaaa atgccggacg taagtccccg atgacgtggg tagtgctcgc agtactcgct
     9961 gtcattgctc tcgtcatcat catgttcgtc atgcgctgag tcagcacatg gcaatccaac
    10021 ggcgcgcccg aatcgtcgat cgcgacggca cgttcggctg gcttcgcagg ataaaggccg
    10081 acgatccgga tcggccgtac gctgtcgtca cgctcggcta cggggccgtc gtcgaggttc
    10141 cgttcgagct tctcgagcac cacgacgacg gcggctacgc gctaggtggg cggtggcgcg
    10201 acttctcgat caaatccgaa gaggtcgcgt cgattcctgt catcgaagag cgcgtgcgag
    10261 tcgacgtgcg accagcaccc gcgcggcagc ttcgcgtgaa acgtcgcgtc gttagtgaac
    10321 ggcagaccgt cgagacgccg gtgtggcacg agcacatcga ggtcgagcgc gtccccgtcg
    10381 acggatcgtt tgtcgatcgt gcgccaaagc cgcgtcggga aggcgacgtc ctcatcattc
    10441 cgtgcgtcga agaggtcgtc atcgtcgaga aacggctgcg cgtgcgagag gagctgcgtg
    10501 tgcgcgtcgt gcgtgagcgc aagttgcacc gccagacggt cgacgtgcgt cgtcacgaga
    10561 tcgagatcga cgagagctac gaggaacccg aaccaacacc actcgtcaaa ggagagtgac
    10621 tcatgaaaac gatcatcgcc gcgttcagcg atcagacagc cgcgcagaac gcgatcaatt
    10681 ttttgaacag caagggcgtc accgacgcac aactgatgag ctcgaacgca ggcggcgaca
    10741 tgatgtcgac gtttcgcacg ctcaacgttc cagaggaacg cgcacagatc tattcggagg
    10801 tgatgcgccg tggcgctctt ctgatcgtcg cccacgtcga ggatgatgcg cggtcgctcg
    10861 cgcacgatct cgacaaaatg ggatcacttg atctcgacca ggcggcgagc cgatggcgtg
    10921 agtcgggatg gaccggctac gacgccagcg caggaccgtt cgacgccacg gcgtccgcga
    10981 cggagcggag tgcgctcgtc tccgagagcg ggagacgaag ggacagcgcg ggtgcgcgcg
    11041 atctggacgt catcgaagag cgcgtgaccg tcggcaagcg cgaggtttcc aaaggcggcg
    11101 tacgcgtccg tacgtctatc tccgagcgcc ccgtcgagga acaggtccag cttaccgagg
    11161 agcgcatcga catccagcgt gagcgtgtcg acgaggcgat ctcgcccaat gccgcggatg
    11221 cgggactgac cgaggatgaa ttcgtcgtca cggcgcgcgg cgaggaggcg gtcgtcggca
    11281 aggaagcacg catcgtcgag cgcgtccacg tcggcaagca ggccgacacc cgcaccgaga
    11341 cgatccgcga gaccgagcgg cgtcgcgacg tagaggtcga gccgatcgaa gagacgcgtg
    11401 accgccgacg ctagctgcta ggtcacagcg aaagcgcggt cgcgatggcg gcggcggact
    11461 tcgggcgctg cgtgatgtcg acgcggaggc agttccgcac gacgtcgtcc cacgttgcat
    11521 cgatctcggc gcgtcgtgcg cgcatcgacg gtggctcgcc ggtgagacgt gcgtgcgcga
    11581 gctcgagcgg tgtcgcgccg tgccacggca gatcgccggt gagcagctcg tagacgatca
    11641 caccgaacga aaatagatcg gtgccttcga ggatcgttcc gcccgtgact tgctcgggcg
    11701 ccatgtacgc cggcgtgccg atgagatggg gcatgtcgca cgcgcagccg agcgtcgcgc
    11761 agcacggaag ggcgaggccg aagtcggtga cgacgacgcg gttgtttgcg ccgatcagca
    11821 cgttcgatgg tttgagatca gcgtggatca cgtccgcggc atgggcggcg ccgatgccgg
    11881 cgaggagctg gcgcacgatc ggttggatct cgccgatcga gaggcagccg cgcgttgcga
    11941 ggtgcgcctt gagcgtctcg cccgcgtgca gctccatcgt gtagaacacg cggtcgccgt
    12001 cggtgccgac atcgaagacg cggcagacgt tggcgtgcgt gacgcgacga gcgagccgga
    12061 tctcgtcggc gaagcgcgcc tgtgcgcccg gcttcgccat caaatccgcg cgcagcagct
    12121 tgacggcgac cggatcgccg agcagctggt cgtcggcctc gtagacctcg cccatgccgc
    12181 ctgccgcgac gaaccgcttg atgcggtacc gaccggctag cgtcgagccc ggcgcgatcg
    12241 gctcgccgac caagcacggg cccggcgagt gatggcgcgc atcggtgacc gtgacctccc
    12301 gcagtgcctg caccagcaca gaatacagcc tcccccgagt ctgcgtaggt acagatgcac
    12361 gacgtcgact ggtacagatc agatgcggtg cgggcgatac acgatcgtcg cgctatcgca
    12421 ggtgcgcagc gaatcgggcc ccggcgacgc ctcgaactgc cagtcgacga tgtcggccat
    12481 gaccgcctgg tcgaactgct cgagcgacga gccgcgctct agcttggcct cgacgacctt
    12541 gccggccggc gacacgcagt agtggatgtc gacgctcgcc tgcgggccga gccgcgactc
    12601 gacgacacgc gcgatgcgat cggcgctcgg cagcatcggc tcgatcgctt gtgggacaag
    12661 gcggacggtg tcgtcaccgg gcagcggcga caagacgacc tttgcatgcg gaacagcagg
    12721 ctcacgatcg gcgcccagca caccggtcgt cgcacacccc gaaagaagaa ctgcatagag
    12781 acagcgttga aaccccatga gtacggcgct tcatcaagca acgtgccacg cgcgtcgcga
    12841 tccaacgtgc tgcaatcgtg cacgcggttg cacgagatcg ctgtcgctgc gacgtcgcat
    12901 gtctgtcgcg aatgtcgcag ctactgcatc gccgcgctcg gcagcggatg cgcgacgaag
    12961 aagtcccata gcgcatcggt cgcgatgatg tcgtcgctct tcttgccgag gaacggcagc
    13021 gcgtcgccgc ccggccactg gtgacctccg ccttggatcg tgcacaggac gaggctcgcg
    13081 ttcgccgtgc agcccgcgtg ggtgacgcag ctcgcatcgc catgcgtgta ggtggtcgac
    13141 tccgtcgagc agccgttggt cgcgtcccag aaatttactg tcgccggcac ccacgagtag
    13201 tcgacgagcg tgtcggacgt gccgtgcacc atccacacgg ggaccggccg cgcgggcgtg
    13261 cagttgccct ggagcaggcc cgcgaccgca ccgatggccg cgacccgatc gccgagatcg
    13321 cacgcgatgc gatgcgcgag gtaaccaccg ttcgacagcc ccatcgcgta gatcctcgcg
    13381 gggtcgacgc acagctgcgc ctccgccttg tcgaggatcg cgcgcatgaa gccgacgtca
    13441 tcgacgttat tcgccgccgc cggatcacag cacccaccac cggagttcca cgacgtcggc
    13501 gagcccgtgc cttcgggatg gatcacgatg aagccttcgg cgtcggactt cgcgatcgca
    13561 tgcgcgagcg ccgcttgttg cgagccccag ccactgcggc catgaatgtc gaggacgagc
    13621 ggcgtgcgcg actgcggcga gtagctcgcg ggcacgtgca cgcgcgcgac gcgcatcgcg
    13681 ctgccgacgg tgatgttcca cgtcgcgtcg agcggctgcg cggtcttgcc gtcgcagttc
    13741 gcggcggttg tcgtcgcagc atcgggcatc ggcgtcgtga cggccgcatc cggtgcgtcg
    13801 tcgcccgttc ccatcccgcc gcctcccggc gagcacgcga cgacgaggac ggtcagggca
    13861 gcggatgcgc ggagaagaag tcccataagg cactcgtcgt atcgaggttg tcgctcttcg
    13921 taccgaggaa cggaaaggtg ctgcctccgc ccggccattg atgcccgccg tcctgcaccg
    13981 tgcacagcac gacatccgcg ccacccgtgc agccaccgtg ggtgacacac gtgacgtcac
    14041 ctttctgata gctctgctgc atcgtcgtgc acttgttctt cgccttccag aagtcgacgc
    14101 cctgcgggac gtactgatac tgcgagagcg tatccgccgt cccgttcacc atcatgatcg
    14161 gaaccggccg cgacggcgcg caaccttgga acagcaacag tccggcaacc ggaccgacgg
    14221 ccgcaaaccg atcagcgagc tcgcatgcga tgcgctgtga ttcgtacgcg ccgttcgaca
    14281 tgcccatcat gtagacgcgc ttcgtatcga cgcacagctt cgcctcgacc tcgtcgagca
    14341 gcttcgtcat gaatgcggtg tcgtcgatct tgttcgtcga cgcgggatcg cagcacgtgc
    14401 ccgagttcca cgcggtcggc gacgtgcccg actcgggaaa cacgacgatg aaaccctcgg
    14461 cgtcggactt cgctttcgag ttcgacagcg acatctcgcc cgacgcgttc tgcgtgcgcc
    14521 cgtgaacatc gaagacgacg ggcgtcgcct tctgcggatc gtagctcgca ggcacgtgga
    14581 cgcgcgcgac gcgcatcgcg ccgcccgcca tgatgttcca cgtcgcgtcg agaggctgcg
    14641 tggtcttgcc ctcgcacgtc atgctcgaca tcccgcccgt cgacgaatcc gccatcatcg
    14701 tcgtcgccgc atcgacgtca gcctgctgtt gcgaacccga gccgctacac ccgacgacca
    14761 cgaccgcgag caagatcctc atcgcgagcg tcgtacacgc caccgctatg cacgatcaac
    14821 gacagatcgt gcacgccgtt gcacgacgct cgcctcgcgt ccgctctccg cggcgcggcc
    14881 ccgacgaggc tcgcgtccgc gcgcggcggc gggcggcttc gcggcagggg cccgacgagg
    14941 gctcgcgtcc gcgcgcgggt gggcggctcc gcggcagggc cccgtcgccg tgtcagtgcg
    15001 gtggggcggg cagcgcggtg ggggcgctag tcacgagcca gggtgcgggg cgcgagcggc
    15061 acgtcgggtt acacgtcgcc gaagccctgc cgcttcgcca cccgcccgcg cgcggccgcg
    15121 agccaagcac tcgcggacga cacgcaggct tacccaatct gtccgggcta gcgacagctc
    15181 tcgagggcca catgccagct acgcgatgcg gggtagcgag agctcgcgag gccgacacgc
    15241 cgggttcagc cgacgagggc tagcaggcgc tcggggtcgt cggactcgat ccagtcgact
    15301 tcgagggaga ccaggcgctc gacctcggcg gccgtcatcg cgcttggcga gatcggcttt
    15361 cccgtcgtgg agccgagcgg gaacagcgtg tgcgtaccga tggcgattcc gaggtcgtgc
    15421 aggcgtttga ctgtttggct gccgacgatc gtgtgctcgg cgccgacgag ccgcgtgccg
    15481 aggagcttgc cgacgaggtt cgtctcgagg atgcgattga gcaggcgccg cgcgttcgtg
    15541 cggatgtggc cctcggccgg cagcgagagc ttcgcgcgga cgtggggcag tcgatcgagg
    15601 aagggggccg accagtcgag catgctgtcg tcgaagcaga agccggtcgc gagcttgggg
    15661 tgtgcggcgg tggccgcacg cagcttgcgc ggatcgaagc tcgtcaggat taccgagtcc
    15721 tcggcgtttg cggcagcgac cacgtcggcc gtgacacgcg cgacctccgt cgaccaccaa
    15781 ccgcgcatgt tgatcttcag ctcgacgttg atcgcgactt tgcccgcaac ctccgcgagt
    15841 acttccgcga gcagcgggat tcgctcttcg ctctcgtagc gaatgatcgt gcgctggcca
    15901 tgcacgtcga cgcccatcgg cagctcgcgt ttgatccgca gcttcgacac ctcgtcccac
    15961 gggacttgcc agacgtagcg cgagtcgccg gtcagtcggc cgaggtgacc gtcgtgcagc
    16021 actaccgctt tgcgatcttt cgtgagctga acgtcgagct cgatcgccgg aacgccgagc
    16081 tggatggcgc gccgaaatcc agcgagcgta ttttcctgat gcagcatcgg aacgccgcgg
    16141 tgtccgacca ccatcggctt gttcttgccg gccaggaacg ggttagacac cgccgcgcgc
    16201 atgtgtttcc cttaaacctt accctgatgt tgctcgatcg acgacgattc ctcgtgggca
    16261 gcgccgcgag cacgctgctg ctcgcctgcc aaaagtcggc gaagacggcg accctccgcg
    16321 aggtcgatca cgtcgtcgct gcgcaggcga gccgcgatgg agccggggtc gcgttgcgac
    16381 gaagcatcgg ctcgggtgtg ctccccaacc tcgatccgtt cctgctgctg gacgagatgc
    16441 acagctccga cccggccgat tatgtggcgg gctttccgca acatccgcat cgcggcttcg
    16501 agacggtctc ctacgtgctg aggggcggct tccagcaccg cgacagcgtc ggcaacagcg
    16561 ggctcatcgc cgatggcgg
//
LOCUS       DATLBZ010000304         3103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6131912, whole genome shotgun sequence.
ACCESSION   DATLBZ010000304 DATLBZ010000000
VERSION     DATLBZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6131912"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VMZ53_15635"
     CDS             complement(<1..663)
                     /locus_tag="VMZ53_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29937.1"
                     /translation="MSSSDGDDPTKKEPDAGATMAGGFAPPRPGKTVPPPRSVPGAAP
                     YRPSSPATPYQPPAQTPAGHPQQPIASPQQPAASPQQPVAPQQPPAPYPLSQTPQPHQ
                     VPQQPDAPSPFAADPSLGKPSHSSAGQWSPAQSSLPLPAQQVAVPEARLLESESSPIE
                     QIAEVAQELPGRFMGFLKISAKRAFRLRIEPTEVLPDERDMLHQANPPVTETNLMAFL
                     AWR"
     gene            complement(723..947)
                     /locus_tag="VMZ53_15640"
     CDS             complement(723..947)
                     /locus_tag="VMZ53_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29938.1"
                     /translation="MSDECAAFEEATVLEPPLFDDDSLVTSPFTMALSGDAPTTIFDP
                     PKRPKMLARGSGVMRSDLERSYAAAMAQFR"
     gene            complement(1161..2663)
                     /locus_tag="VMZ53_15645"
     CDS             complement(1161..2663)
                     /locus_tag="VMZ53_15645"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS29939.1"
                     /translation="MLKTSLVCVVLFTAACGKKAEEKKAPEPTEKIVEKQTKEPTAPV
                     NAKRVEVTTKSPEAKAEYEKGHELATNLRSEEAIEHYKKALELDPEFALAMAELGNVT
                     PGGEGTDLLNKVTPLMSKLDEPQQKLIEAYQGLRAGDPKKAIAALDRVLELAPGQWEA
                     DLMIANLSNGRLDDATAIKHLEHALSVNPNLPQAQNGLAYSYANQKNWDKAIAAAKKQ
                     VELLPKEPNPADTLGEVEMWAGKFEESEKDFLKATELSANFPPAWQGVMLARAYKGDF
                     KGAYEANAKREQSTTPGVKNEAMLDRAWLQFTENKPEEAAKTLDAAEKDADAKKHPVY
                     AFMAADRAWMQMQQGKFADAAKSNEMMKSRLELLAGDARSGATTRYRVQVLRLAALQG
                     KPAADADKILADAAEDGKRLGDDKVEAATQAYMRGLAAWAKGGAKDGPKAAIAELSKC
                     GEKMIICRYDLALATRKSGDAAGADAIEKQLAATPIRALEGIYVKTHPTK"
     gene            complement(2677..>3103)
                     /locus_tag="VMZ53_15650"
     CDS             complement(2677..>3103)
                     /locus_tag="VMZ53_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29940.1"
                     /translation="VEGATSVVDQPRGHDAGGAALEPGPSADNPRGLGPAGTVHCSLD
                     DYGKFLNLHATGEPAGFVTPESMQHLHTPRDGEYAGGWGVVESPKGPILIHSGSNTLW
                     YATALVAPGTRFVFVIATNKFEDGIENELGSLLVRFEQK"
BASE COUNT          443 a         1071 c          965 g          624 t
ORIGIN      
        1 ccgccacgcg aggaacgcca ttagattggt ctcggtgaca ggcgggttcg cctgatgaag
       61 catgtcgcgc tcgtcgggca gcacttccgt cggctcgatg cgcaaacgga acgcgcgctt
      121 cgccgagatc ttcaggaagc ccatgaaacg accggggagc tcctgcgcga cttcagcgat
      181 ctgctcgatc gggctcgact cgctctcgag caagcgtgcc tccggaaccg cgacttgctg
      241 cgccggtagc ggcaacgacg attgcgccgg tgaccattgc cccgcggacg aatgcgacgg
      301 cttgccaagc gacggatccg cggcgaacgg cgatggcgcg tccggttgtt gcggaacttg
      361 atgcggttgc ggcgtctgcg agagtggata cggcgccggc ggctgctgcg gcgcgacggg
      421 ttgctgcgga gacgcggccg gttgttgcgg agacgcgatg ggttgctgcg ggtgacctgc
      481 gggcgtttgc gcgggcggct gatacggcgt tgccggcgac gacggccggt acggcgctgc
      541 acctggcacc gacctcggcg gcggcaccgt cttgccaggc cgcggcggcg cgaagcctcc
      601 ggccatggtc gctcccgcgt ccggctcctt cttcgtcgga tcgtccccat ccgatgacga
      661 catgcgtcgc atcgtactcg atcaccggcg ctacaaccgg ttaggcgacc aagagtgcag
      721 tgctagcgga attgcgccat cgcagctgcg taactgcgct cgagatcact gcgcatgacg
      781 ccggagccgc gcgcgagcat cttcggccgc ttcggcggat cgaagatcgt cgtcggcgca
      841 tcgcccgaca acgccatcgt gaacggcgat gtcacgaggc tgtcatcgtc gaacagcggt
      901 ggctcgagca ccgtcgcctc ctcgaacgcc gcgcattcgt cagacaccgt cacatcctcc
      961 aacgactcca ccgaggtgtc tgtccacacg cgcggcttgc tgtgcaacgc gtgttccgcg
     1021 ctacagcaaa ttcaagctcg cgtgatcacg cgggccgccc aacgcgtggc gatgttcgca
     1081 ggttgcacag tgtgcaatcc gcccacgcgg gactcgagct ggcgactttg tccccgccgc
     1141 cctctgcagg ttgcagccgg ttacttcgtc gggtgcgtct tgacgtagat gccctcgagc
     1201 gcgcgaatcg gcgtcgccgc gagctgcttc tcgatcgcgt cggcgccagc agcatctccg
     1261 gacttgcgcg tcgcgagcgc gaggtcgtag cggcagatga tcatcttctc gccacacttc
     1321 gagagctccg cgattgcggc cttcgggccg tccttcgcgc cgccctttgc ccacgccgcg
     1381 aggccacgca tatacgcctg cgttgccgcc tcgaccttgt cgtcgccgag acgcttaccg
     1441 tcctcggccg cgtctgcgag gatcttgtcg gcgtctgccg ccggcttgcc ctgcagggca
     1501 gcgaggcgaa gcacctgcac gcgatagcgc gtggttgcgc ccgagcgcgc gtcgcccgcg
     1561 aggagctcga ggcgcgactt catcatctcg ttcgacttgg cagcgtcggc gaacttgccc
     1621 tgctgcatct gcatccacgc gcggtcggcc gccatgaacg cgtagaccgg atgcttcttc
     1681 gcatcggcgt ctttctccgc cgcgtcgagc gttttcgctg cctcctcggg cttgttctcg
     1741 gtgaactgca gccacgcgcg atcgagcatc gcttcattct tcacgccggg tgtcgtcgac
     1801 tgctcgcgct ttgcgttcgc ttcgtacgca cccttgaagt cgcctttgta tgcgcgcgcg
     1861 agcatcacgc cctgccacgc gggcggaaag ttcgcgctca gctcggtcgc cttgaggaag
     1921 tccttctccg actcctcgaa cttgcctgcc cacatctcga cctcgccgag cgtatccgca
     1981 gggttcggct ccttcggcag aagctcgact tgcttcttcg cggccgcgat cgccttgtcc
     2041 caattctttt ggttcgcgta cgagtacgcg agaccgttct gcgcctgcgg gaggttcggg
     2101 ttcacgctga gcgcgtgctc gaggtgcttg atcgccgtcg cgtcgtcgag ccgaccgttc
     2161 gacaggtttg cgatcatcag atccgcttcc cactggcccg gcgcgagctc gagcacgcga
     2221 tcgagtgcag caatcgcctt cttcgggtcg cccgcgcgca atccttgata cgcctcgatc
     2281 agcttttgct gcggctcatc gagcttgctc atgagcggcg tgaccttgtt caagaggtcc
     2341 gtgccctcgc cgcccggcgt cacgttgccg agctcggcca tcgcgagcgc gaactcggga
     2401 tcgagctcga gcgccttctt gtagtgctcg atcgcttcct cggacctcaa gtttgtcgcg
     2461 agctcgtggc ccttctcgta ctccgccttc gcttccggcg actttgtcgt gacttcaacg
     2521 cgcttcgcgt tcaccggcgc cgtcggctcc ttcgtctgct tctcgacgat cttctcggtc
     2581 ggctccggcg ccttcttctc ttccgccttc ttgccgcacg ccgccgtgaa caacaccaca
     2641 cacaccagag aggtttttag catccggcga ccatagtcac ttctgctcga agcgcacaag
     2701 gagcgagccc agctcgttct cgatcccgtc ttcgaacttg ttcgtcgcga tgacgaatac
     2761 gaaccgggtc ccgggtgcga caagagctgt cgcataccag agcgtgttgc tgccgctgtg
     2821 aatgaggatg gggcccttcg ggctctcgac gacgccccac ccgcccgcgt actccccatc
     2881 gcggggcgtg tgcaggtgct gcatgctctc gggcgtcacg aatcccgcag gctcaccggt
     2941 cgcgtgcagg ttcaagaatt ttccataatc gtcgagcgag cagtgcaccg tccccgccgg
     3001 tccgagacca cgcggattgt ctgccgatgg tccgggctcg agcgctgcgc cacccgcgtc
     3061 atgtccgcgc ggctgatcga ccacggacgt cgcgccctcg aca
//
LOCUS       DATLBZ010000305        11810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_342834, whole genome shotgun sequence.
ACCESSION   DATLBZ010000305 DATLBZ010000000
VERSION     DATLBZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342834"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VMZ53_15655"
     CDS             complement(<1..1151)
                     /locus_tag="VMZ53_15655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUS29941.1"
                     /translation="MENAPIVVLGGSDEERASLVSQLTSSGLNATGVTKLTSADGLPG
                     LIVITGEAVIELVSEARDVPALAETPILAIVPGLPVTTLAGALAAGATDVVRQPVPSA
                     LLAARCRNCLKLSRRDAAGAQALSKINEILTAEGDDSEALIQVLQITASVLGFDRASL
                     IAHIEGSDHAFVIAASDADPMQRFTLNISEYPEVIEGMRGAVPLLIDDVLTHPTTAAL
                     ADVLAPRKVRGVAVFPVQWRGRSLGAILLRKRLPGTNHVGPRGTELGKLIASITAAHL
                     RHGAVLESLRDQTHRISRARYEAERRLRGIDSLKEHFEAGADGVVVLDDAGRILFVNR
                     AAERITGFARDGLLGSELVDLVPIDQRAMISDAIARVLAGVNVETFDLEL"
     gene            complement(1221..2738)
                     /gene="larC"
                     /locus_tag="VMZ53_15660"
     CDS             complement(1221..2738)
                     /gene="larC"
                     /locus_tag="VMZ53_15660"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HUS29942.1"
                     /translation="MTQQPPSFEPRGQHLHFDCASGAAGDMMLGALIDLGVPLDVIGE
                     ALDAIGAGRQRVMLAKIVKHGIAAIDIKVDTTGHILDAHQHTHTHHGPQQPTRGTTPA
                     AGVPHHHPRPGTPPPVRRAATESGSASGSGKFVGIKFRAETHERHVHVDALGDKVEEV
                     LEYPGAQPQPHAHAHAHYLYADIRWRIARAPLREGTRMRALDIFDRLARAEAKLHGTT
                     IDEVQLHEVGAIDSIVDVVGTAAALDWLQPASVTCAAVAMGHGTLTSAHGTLPVPAPA
                     ALEVLREAQGVMMDGGLPRELCTPTGAAILAHAVTSWSPSPPGRALAVGWGAGDTDLA
                     DRPNVLRATLLAPVTSASGAAVSKGDTVWQIDANLDDMSPELCGPASDALFAAGALDV
                     WWTPITMKKGRPALTLSALVELPRRDAVISSMLRETTTIGVRFTELQRTVLTRKLIEV
                     ETRYGIIPVKVAVDGNTILNAAPEYEACAKAARVHQVPVKLVYSAALAAYDARRP"
     gene            complement(2735..3499)
                     /gene="larB"
                     /locus_tag="VMZ53_15665"
     CDS             complement(2735..3499)
                     /gene="larB"
                     /locus_tag="VMZ53_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HUS29943.1"
                     /translation="MDRSQLEKLLADVRAGAVTPADAANQLAQVPYADQAARDIVVDP
                     HRELRTGVPELVFGASKTAEQIATALQALAKHNGGALATRVDADKARAVRALLPDAAY
                     HDVARALTLGAMPPRPACAPICIVCAGTSDLPVAEEAALVAEFLGAPVTRIADVGVAG
                     LHRLLARVDDIRKADVVIAVAGMEAALPSVLGGLVDRPMIAVPTSVGYGVSLDGLVAL
                     GGLLASCAPGITVVNIDNGVGAAVAAVKIARLVVRP"
     gene            complement(3509..4786)
                     /locus_tag="VMZ53_15670"
     CDS             complement(3509..4786)
                     /locus_tag="VMZ53_15670"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HUS29944.1"
                     /translation="MSGQLVTIGELVAILIACVLVQGFFVSAEVALSACDRNALRARA
                     AGGSAKAARAERMLAVPQVTLATTLVGANLATLVAVLILAMDLMMRGISPMWAPLIVV
                     PPLLVLGHLVPKSIVQAHADGTVDRLATPLRLASYVLRPLVVVVGGFAAALTRVTRTD
                     RKKAFVTRDELALLIESEPTSDKPEIGADEREMIANVFELSEYKAEELMVPLSEVTAL
                     HEDAPVNEAALEVADKQHSRMPIYRARVDDIVGIVHVFDILQAAGGKPPEGKPVPKTV
                     SDLAHSPTYVPGTMRASDLLVQLQSEQQHLAIVVDEYGGAVGIITIEDLLEIIVGDID
                     DEYDVEPSPIRAEKPGVWRVEARTNVARLNAELELGLPESDDYETIAGLLIEKFRRIP
                     DKGESLAIGGVLVEILAATDRAIEAVRITRKKK"
     gene            complement(4786..6060)
                     /locus_tag="VMZ53_15675"
     CDS             complement(4786..6060)
                     /locus_tag="VMZ53_15675"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HUS29945.1"
                     /translation="MSWLSQLTVAIPLAALCVLVSGFCSGTEVALFSLRRVDREQLAR
                     SELRLDRRILKLLERPRRLIATVLIGNETFNSLLAVLALVVVSQLDGPRSPWLMGLIA
                     LGVSLPLIVLVSEVTSKTLAAKAPLLWSRICVLPLSGFAALVTPLRLVVNFISEVFLR
                     PLGDAVRTRPARDLSEQEFKSLVDAGSAQGQVDARERRLIHRVFEFNDKNVGQVMTPR
                     DRMFALAYDLPMQRLVKEVASHGFSRVPIYQKSVDNVRGILNAKDLVRYTPGQGPLRP
                     LGDLLHEPLFVPRTTPVKRLFLTFKQKKVHMAIVVSEYGKVLGIVTMDDLLAQIFGHM
                     RDERADLQASLTGIKVQGRARTPGVGVSETGPAPKQEATDLESDLATGALRSLHDESG
                     MLPIPVQPIHPTDEVTPPAVDVDELQAREKDN"
     gene            complement(6057..7196)
                     /locus_tag="VMZ53_15680"
     CDS             complement(6057..7196)
                     /locus_tag="VMZ53_15680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS29946.1"
                     /translation="MSGTGPLDGPIELARLVPPNELQELIDAVRRALGAELAVFDPKG
                     QRLAGAESVGPPTREVTHDGEPLAKIAATGDRADAALDLAADTLGLLVHHANARELAA
                     TMHEEAMRLTFAELTEHNQRLQKAVARLEELDRLKSNFLATMSHELRTPLTSVIGYAE
                     MMAEGLAGPITGDQRDYLTTILSKADQLLGMITSVLDVAALETGPLALERSRLSLADI
                     VASEVATYSSQAGKRGIAIQLEARGDSIVVGDRKKIRQVVSSLISNAVKFTPDRGRVG
                     VAIRPGPLSPHEQPDGDARAIQLVVSDSGIGISREQVNKIFEPFFQVDSSSTRAFGGT
                     GLGLTLAKAYVEAHGGRIWVDTTPGQGSTFVATFPQDAAVEVTTS"
     gene            complement(7233..7892)
                     /locus_tag="VMZ53_15685"
     CDS             complement(7233..7892)
                     /locus_tag="VMZ53_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HUS29947.1"
                     /translation="MAQVDFAERQLTLKLVYYGPPLSGKTSNLRALHTKVDKLNRGRL
                     MTLDTRDDRTLFFDLLPIFFRTSSFSMRIKVYTVPGQPVHEATRKVVLAGADGVVFVA
                     DSSPDQREANRASWHNLTANLTAFALDRIPIVVQYNKRDLDNAVPIDQADRFGDPNRK
                     ILEARAKEGFGVVQTFFELVGGAWTKLDRDLGLEKKLGIDAQAFRKSLAEHVGVTESI
                     G"
     gene            complement(7908..8279)
                     /locus_tag="VMZ53_15690"
     CDS             complement(7908..8279)
                     /locus_tag="VMZ53_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29948.1"
                     /translation="MRAAVEAAWQRVVESWDDQTRHDAFIGLVAQYGDFAWAASKYKE
                     RAGDAIADKHLEKIRKAATATMFATASKKPEGGAPYKSTMVIFAVLILMMLIALIGVK
                     LMHDTRPHPEPPPPPTTPARH"
     gene            complement(8289..9167)
                     /locus_tag="VMZ53_15695"
     CDS             complement(8289..9167)
                     /locus_tag="VMZ53_15695"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HUS29949.1"
                     /translation="MSLGRDALISLGLFVTCCITTTIVGGFAFGATLMGILLCHEAGH
                     YVVGRMHGEDVSLPYFIPMPPFLTLGTLGAVIRMRTPIRDRKKLFDVGAAGPVAGLVV
                     AIPLLLVGLSLSDVGPIPKDSAIEGNSILYALLKYATFGRWLPGDNIDVHLHPMALAA
                     WVGLLVTMINLMPIGQLDGGHIARAALGQSHEKWSQRLHVALPVIGASVGTAMLVVAH
                     NAGKGWSESIKYASSGVIPWMIWTLMLGWMRRSAGEYHPAVDDEPLSGWRRAAAVGML
                     LLFITILTPVPWRSVL"
     gene            9184..10317
                     /locus_tag="VMZ53_15700"
     CDS             9184..10317
                     /locus_tag="VMZ53_15700"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HUS29950.1"
                     /translation="MGTVKSVQLFFQEGTSDKLYNAQIVEDGGGYTVAVQWGRRGSSL
                     QTGSKAVKVSRAKAEATFDKIVREKRSKGYEEIGGTVKPAAVAPPAGSGSASRAGAGT
                     KRAVVGHAAQLLTPIEDDELDRFLSDEDVIAQQKIDGVRVLVHITPAGELVATNRHGQ
                     PTQMAGGNALAGLAYLPKATVVDGEILDNAYWIFDVLQVAGTDVRTHGYLDRWKLLDE
                     ELEPALTGTAQILPMAKGAKAKRTLHDKLRRVGAEGIVFKQRDAPYAHGRVTTQRKYK
                     FIKTADVIILENVGNAYKMAVMDGARLFEVGNVFAGTTNASRKDLDARLARGERPVAE
                     VRYLYATDDHQLFQPVFVTLRDDKLARSCLRSQLLETSRRVIV"
     gene            10351..11262
                     /locus_tag="VMZ53_15705"
     CDS             10351..11262
                     /locus_tag="VMZ53_15705"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUS29951.1"
                     /translation="MRARIKVGTTTWAGRGLVATGWYPRDVKTSEERLHYYASQFPIV
                     ENEAGYYAILTPEQVALWADRTPDGFTMNMKAHALFTAHYADPRRLPRDVVEALPRGV
                     RAKPHVYPRDLSVATMRDLERRFRDALLPLHEAGKLGVVLFQFPMWFVISREHKAELS
                     RLRESFAPYRVAVELRNKTWLSEENRDETLAHLRKEDIAYCCVDEVGPIPPIAAATAD
                     IAVVRMHGRNASRFKGRRSLPEGARYVYTQAELREWVPRIRELAKHAREVHVLFNNAV
                     ADYAVRGAHDLVELLEGARGVTLAKAG"
     gene            complement(11259..>11810)
                     /locus_tag="VMZ53_15710"
     CDS             complement(11259..>11810)
                     /locus_tag="VMZ53_15710"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS29952.1"
                     /translation="PDVPFSLLYIPDRYDRAAIVAAFLPYYGVELRTAENMNSAQLQR
                     KHGGHVPTVVQLVGGAGWHHPSLPVRGGEAVQGALIVDAFAGGDGGEIAADFVAAFQQ
                     KTNRTPSAAGAEAFDAATLIAKARAAVGMTSPDPRGALRTAIARAKLDDGACGPASMD
                     GDGELAREPALLEVQADQIVLVP"
BASE COUNT         1993 a         3906 c         4008 g         1903 t
ORIGIN      
        1 agctcgagat cgaacgtctc gacgttgaca cccgcgagca ccctcgcgat cgcgtcggag
       61 atcatcgctc gctggtcgat gggcacgaga tcgacgagct cgctgccgag cagaccgtcg
      121 cgcgcaaagc cggtgatgcg ctccgccgcg cgattgacga acaagatccg tccagcgtcg
      181 tcgaggacga ctacgccgtc ggcgcccgcc tcgaagtgct ccttcagcga atcgataccg
      241 cgtagcctcc gctctgcctc gtagcgcgcg cgcgagatgc gatgcgtctg atcgcgcaag
      301 ctctcgagca ccgcaccatg ccgcaagtgc gccgccgtga tgctcgcgat gagcttgccg
      361 agctccgtcc ctcgcggccc gacgtgattc gtccccggca agcgcttgcg caacaggatc
      421 gcgccgagcg atcgcccgcg ccattgcacg ggaaacactg cgacgccgcg caccttcctc
      481 ggcgccagca catccgctaa cgccgccgtc gtcgggtgcg tgagcacgtc atcgatgagc
      541 agcggcaccg cgccgcgcat gccctcgatc acctcgggat actcgctgat gttcagcgtg
      601 aagcgctgca tcggatcggc gtcgctcgcc gcgatcacga acgcatgatc gctgccctcg
      661 atgtgcgcga tgagcgatgc gcgatcgaag ccgagcacgc tcgcggtaat ctgcagcacc
      721 tgaatgagcg cctcggagtc atcgccctcc gcggtcagaa tctcgttgat cttcgacagc
      781 gcctgcgcgc ccgccgcatc gcgccgcgag agcttcagac agttgcgaca acgcgctgcg
      841 agcaaagcgc tcggcaccgg ctgccgcacg acatccgtcg cacccgccgc gagcgccccg
      901 gccaaggtcg tcaccggcag cccgggcacg atcgcgagaa tcggcgtctc ggcgagcgcc
      961 ggcacatcgc gcgcttcgga cacgagctcg atgaccgcct cgccagtgat gacgatgagc
     1021 cccggcagcc catccgcgga agtcagcttc gtgacgccag tagcgttcag gccgctcgac
     1081 gtgagctggc tgacgagcga cgcgcgctcc tcatcgctgc cgcccaaaac gacgatcggc
     1141 gcattctcca caggcggtta gagggtacct gtgaacgccg ccgccgtcga cccaccgtcg
     1201 aggctggtcg agcaagagag ctacggacgg cgagcgtcgt acgccgcgag cgcggcgctg
     1261 tagacgagct tcacgggcac ttggtgtacg cgagccgcct tggcgcacgc ctcgtactcg
     1321 ggtgcggcgt tgaggatcgt gttgccgtcg acggcgactt tgaccggaat gatgccgtag
     1381 cgcgtctcga cctcgatcag cttacgcgtc agcacggtcc gctgcagctc ggtgaagcgg
     1441 acgccgatcg ttgttgtctc gcgtagcatc gagctgatca cggcgtcgcg gcgcggcagc
     1501 tcgacgagcg cggagagcgt gagcgctggc cggcccttct tcatcgtgat cggcgtccac
     1561 cacacgtcga gcgcgcccgc ggcgaacagc gcatcggatg cggggccgca gagctcgggg
     1621 ctcatgtcgt cgaggtttgc gtcgatctgc cagacggtgt cgcccttgct gacggcggcg
     1681 cccgacgcag aggttacggg ggcgagcagc gttgcgcgca agacattcgg gcggtcggct
     1741 agatcggtgt cgcctgctcc ccagccgacg gcaagcgcgc gtcccggcgg agagggtgac
     1801 cacgacgtga cggcgtgcgc gaggatggcg gcgcccgtcg gcgtgcagag ctcgcgaggc
     1861 agaccgccgt ccatcatcac gccctgcgct tcgcggagga cttcgagcgc ggcgggcgcg
     1921 ggaacgggaa gcgtgccgtg cgcggacgtg agcgtgccgt ggcccatcgc gacggcggcg
     1981 cacgtaacgc tggcgggctg gagccagtcg agcgcggccg ccgtgccgac cacgtcgacg
     2041 atcgaatcga tcgcgccgac ttcgtggagt tggacctcgt cgatcgtcgt gccgtgcagc
     2101 ttggcttcgg cgcgagcgag gcgatcgaag atatcgagcg cgcgcatgcg agtgccctcg
     2161 cgcagcggtg cgcgagcgat gcgccagcga atgtcggcgt agagatagtg cgcgtgggcg
     2221 tgcgcgtgcg gttgtggctg tgcacctggg tattcgagga cctcttcgac tttgtcgccg
     2281 agcgcgtcga cgtgaacgtg ccgctcgtgc gtttccgcgc ggaacttgat gccgacgaac
     2341 ttgccggagc cggacgcgga gcctgattcc gtcgcagctc ggcggaccgg cggtggtgtt
     2401 ccggggcgcg gatgatgatg cggcacgccg gcggctggag tcgtcccgcg cgtgggctgc
     2461 tgcgggccgt ggtgcgtgtg cgtgtgctgg tgcgcgtcga ggatgtggcc cgtcgtgtcg
     2521 accttgatgt cgatcgcggc gatgccgtgc ttgacgatct tcgcgagcat cacgcgttgg
     2581 cgacctgcgc cgatcgcatc gagcgcttcg ccgatcacgt cgagcggaac gccgagatcg
     2641 atcagcgcgc cgagcatcat gtcgccggcg gcgccgctcg cgcagtcgaa gtggaggtgc
     2701 tggccgcgag gctcgaagga aggcggttgc tgcgtcatgg gcgtactacc aatcgagcga
     2761 tcttgaccgc cgcgaccgca gcgccgacgc cgttatcgat attgacgacc gtgatgcccg
     2821 gcgcgcacga cgcgagcagg ccgccgaggg cgacgaggcc atcgagcgag acgccgtagc
     2881 cgacgctcgt cggtaccgcg atcatgggcc gatcgacgag accaccgagc acggacggca
     2941 gcgcggcttc catgccggcg accgcgatca cgacgtcggc cttgcgaatg tcatcgacgc
     3001 gcgcgagcag acggtggagg ccggcgacgc caacatcggc gatgcgcgtg accggcgcgc
     3061 cgaggaactc tgcgacgagc gctgcttcct cggcgaccgg cagatcactc gtgcctgcgc
     3121 agacgatgca gatcggcgcg catgcgggac gcggcggcat ggctccgagc gtcagtgcgc
     3181 gcgcgacgtc gtgatacgcg gcatcgggca gcagagcgcg aaccgcgcga gctttgtcgg
     3241 catcgacgcg cgtcgcgagt gcaccgccgt tgtgcttcgc gagagcttgc agcgctgttg
     3301 cgatctgctc ggccgttttc gaggcgccga acacgagctc gggcacgccg gtacgcagct
     3361 cgcgatgggg atcgacgacg atgtcgcgcg ccgcttggtc ggcataagga acctgcgcga
     3421 gctgattcgc agcgtcggca ggcgtcactg cgccggcgcg tacgtcggcg aggagctttt
     3481 cgagctggct gcgatccacc gccgaacctt acttcttctt gcgcgtgatg cgcactgcct
     3541 cgatggcacg gtccgttgcg gccaggattt cgacgagcac accgccgatg gcgagcgact
     3601 cgcccttgtc ggggatgcgg cggaacttct cgatgagcag gcccgcgatc gtctcgtagt
     3661 cgtcactctc gggcaggccg agctcgagct ccgcgttgag gcgcgcaacg ttcgtgcgtg
     3721 cttcgacgcg ccacacaccc ggcttctcgg cccggatggg cgacggctcg acgtcatact
     3781 cgtcgtcgat gtcaccgacg atgatctcga gcaggtcctc gatcgtgata atgccgacgg
     3841 cgccgccgta ctcgtcgacg acgatcgcga ggtgttgctg ctcgctctgc agctggacga
     3901 gcaagtccga cgcgcgcatc gtgcccggca cgtacgtcgg cgagtgcgcg aggtccgaca
     3961 ccgtcttcgg cacaggcttg ccttcgggcg gcttgccacc tgcggcctgc agaatgtcga
     4021 acacgtggac gatgccgacg atgtcatcga cacgcgcgcg atagatcggc atgcgcgagt
     4081 gctgcttgtc agcgacttcg agcgcagctt cgttgaccgg cgcatcttcg tgcagcgcgg
     4141 tgacctcgga gagcgggacc atgagctcct cggccttgta ctcggagagc tcgaacacgt
     4201 tcgcgatcat ctcgcgctcg tcggcgccga tctccggctt gtcactcgtc ggctcgctct
     4261 cgatgagcag cgcgagctcg tcgcgcgtca cgaacgcctt cttgcgatct gtgcgcgtca
     4321 cgcgcgtgag cgccgcggcg aatccaccga cgacgacgac gagcggccgc agcacgtacg
     4381 atgcgaggcg cagcggcgtc gcgaggcgat cgaccgtacc gtcggcgtgc gcttgcacga
     4441 tcgacttcgg cacgaggtga ccgagcacga gcagcggagg cacgacgatg agcggagccc
     4501 acatcggcga gatgccgcgc atcatgagat ccatcgcgag gatgagcacc gcgacgagcg
     4561 tcgcgaggtt tgcgccgacg agcgtcgtcg cgagcgtgac ctgcggaacg gcgagcatcc
     4621 tctcggcacg cgccgccttc gccgagccac ccgccgcacg agctcgaagc gcattgcgat
     4681 cgcaggccga cagcgcgacc tctgcgctga cgaagaagcc ctgcacgagg acgcacgcga
     4741 tcaggatcgc gacgagctca ccgatggtga cgagctggcc cgacactaat tatccttctc
     4801 ccgcgcctgc agctcgtcga catcgaccgc tggtggagtg acctcgtcgg tcggatggat
     4861 cggttggacg ggaatcggga gcatgccgga ctcgtcgtgc agcgaccgca gcgcgccggt
     4921 ggcgaggtcg ctctcgaggt ccgtcgcttc ttgcttcggt gcgggccccg tctcggacac
     4981 gccaacaccc ggcgtgcgcg cgcgaccttg gaccttgatg ccggtcagcg atgcctgcag
     5041 gtcggcgcgc tcgtcgcgca tatggccgaa gatctgcgcg agcaaatcgt ccatcgtgac
     5101 gatgccgagc accttgccgt actcggacac gacgatcgcc atgtgcactt tcttctgctt
     5161 gaacgtgagg aacaggcgct tgaccggcgt cgtgcgcggg acgaacagcg gctcgtggag
     5221 cagatcgccg agcggtctca gtggtccctg ccccggcgtg tagcgcacga ggtccttcgc
     5281 gttgaggatg ccgcgcacgt tgtcgacgga cttctggtag atcggcacgc gcgagaagcc
     5341 gtggcttgcc acttctttga cgaggcgctg catcggcaag tcgtacgcga gcgcgaacat
     5401 gcgatcgcga ggcgtcatca cttggccgac attcttgtcg ttgaactcga acacgcgatg
     5461 gatcagccgg cgctcgcgcg cgtcgacttg gccctgcgcc gagcccgcgt cgacgagaga
     5521 cttgaactcc tgctcggaca ggtcgcgcgc cggccgcgtg cgcaccgcgt cgccgagcgg
     5581 ccgcaggaac acctcggaga tgaagttgac gacaaggcga agcggcgtga cgagcgccgc
     5641 aaagcccgag agcggcagca cgcagatgcg cgaccacaat agtggcgcct ttgccgcgag
     5701 cgtcttcgac gtgacctcgg agacgagcac gatcagcggc agcgacacgc cgagcgcgat
     5761 gaggcccatg agccatggcg agcgcgggcc gtcgagctgg ctgacgacga cgagcgcgag
     5821 caccgccagc aagctgttga acgtctcgtt gccgatcagc accgtggcga tcaggcggcg
     5881 cggacgctcg aggagcttga ggatgcggcg atcgagccga agctcgctgc gcgcgagttg
     5941 ctcgcggtcg acgcggcgga gcgagaacaa cgcgacctcg gtgccggagc agaaaccgga
     6001 gacgagcacg cacagcgccg cgagcggaat ggcgacggtc agctgcgaca gccagctcac
     6061 gaggtcgtga cctccaccgc cgcgtcctgc ggaaatgtgg cgacgaacgt cgagccctgc
     6121 cccggtgtcg tgtcgaccca gatgcggccg ccgtgcgctt cgacgtacgc cttcgcgagc
     6181 gtgagcccga ggccagtgcc gccgaaggca cgcgtcgacg acgaatcgac ctggaagaac
     6241 ggctcgaaga tcttgttcac ctgctcgcgc gagataccga tgccgctgtc gctgacgacg
     6301 agctggatcg cacgcgcgtc gccgtccggc tgctcgtgcg gcgacagcgg gcccggccgg
     6361 atcgcgacgc cgacccgtcc tcgatcaggc gtgaacttca ccgcgttcga gatcaggctc
     6421 gagacaactt ggcggatctt cttgcggtcg ccgacgacga tcgaatcacc gcgcgcttcg
     6481 agttggatcg cgatgccgcg cttgcccgct tgcgacgagt acgtcgcgac ttcgcttgcg
     6541 acgatatctg cgagagacag ccggctgcgt tcgagcgcga gcggcccggt ctcgagcgca
     6601 gcgacgtcga gcacgctcgt gatcatgccg agcagctggt cggccttgct taggatcgtc
     6661 gtcaggtaat cgcgttgatc gccggtgatc gggccggcga ggccctcggc catcatctcg
     6721 gcgtagccga tcacactcgt cagcggcgtg cgcagctcgt gcgacatcgt cgcgaggaag
     6781 ttcgacttga ggcgatcgag ctcttcgagg cgcgcgaccg ctttctgaag gcgctggttg
     6841 tgctcggtca gctccgcgaa tgtcagccgc atggcctctt cgtgcatcgt tgcggcgagc
     6901 tcgcgcgcgt tcgcgtgatg gacgagcagc ccgagtgtgt cggccgcgag atcgagcgcg
     6961 gcgtcggcgc ggtctccggt cgcggcgatc ttcgcgagcg gctcgccgtc gtgcgtcacc
     7021 tcgcgggttg gcggccccac gctctcggca cccgcgagtc gttgcccctt cggatcgaac
     7081 acggcgagct ccgctccgag cgcgcggcgc actgcgtcga tcagctcctg cagctcgttt
     7141 ggtggaacca gacgggctag ctcgattgga ccatcgagag gtccagttcc gctcaccgaa
     7201 acatagtagc gcattcgcgg ctaagccgct gtctacccaa tgctttcggt gactccgacg
     7261 tgctcggcga gtgacttcct gaacgcctgt gcatcgatgc cgagcttctt ctcgaggccg
     7321 agatcccgat cgagcttcgt ccaggcgccg ccgacgagct cgaagaacgt ctgaacaaca
     7381 ccgaagcctt ccttcgcgcg cgcctccaga atcttccgat tcgggtcgcc gaagcgatcg
     7441 gcctgatcga tgggcaccgc gttgtcgaga tcgcgcttgt tgtactgaac gacgatcggg
     7501 atccgatcga gcgcgaacgc tgtgaggttc gcggtgaggt tgtgccacga cgcgcgattc
     7561 gcctcgcgct gatccggcga gctgtcggcg acgaacacga cgccatcggc gcctgcgagg
     7621 acgaccttgc gcgtggcctc gtgcacgggc tggccgggca ccgtgtacac cttgatccgc
     7681 atcgagaagc tcgatgtgcg aaagaagatc ggcaagaggt cgaagaagag cgtgcggtcg
     7741 tcacgcgtgt cgagcgtcat gaggcgaccg cgattgagct tgtcgacctt cgtgtgaagc
     7801 gcgcgcagat tgctcgtctt gcccgacagc ggtggcccgt aatagacgag cttcagcgtc
     7861 agctggcgct cggcaaaatc aacctgggcc acggactaca gagtagctca gtgacgcgcc
     7921 ggtgtggtgg gaggcggcgg tggctcggga tgcggtcggg tatcgtgcat gagcttcacg
     7981 ccgatcagcg cgatgagcat catgaggatg agcaccgcga agatcaccat cgtgctcttg
     8041 tacggcgccc cgccctcggg tttcttcgac gcggtcgcaa acatcgtcgc agtggcggcc
     8101 ttgcggatct tctcgaggtg cttgtcggcg atcgcatcgc ctgcgcgctc tttgtacttg
     8161 ctcgccgccc acgcgaagtc accgtactgc gcgacgagac cgatgaacgc gtcatgtcgg
     8221 gtctggtcat cccagctctc gacgacacgc tgccaggcag cctctactgc ggctcgcacg
     8281 cctctagctt acagcaccga acgccacggc accggtgtga ggatcgtgat gaagagcagg
     8341 agcatcccga cggccgccgc gcggcgccag cccgacagtg gctcgtcgtc gacggcaggg
     8401 tggtactcgc ccgcgctgcg ccgcatccag ccgagcatga gcgtccagat catccacggg
     8461 atgacgccgc tcgatgcgta cttgatcgat tcgctccagc ctttgccggc attgtgtgcg
     8521 acgacgagca tggcggtgcc gacgctcgca ccgatcacgg gcagcgcgac gtgcaggcgt
     8581 tgcgaccact tctcgtgcga ttggccgagc gccgcgcgag cgatgtgacc accgtcgagc
     8641 tggccgatcg gcatcaggtt gatcatcgtc acgagtaggc ccacccacgc ggcgagcgcc
     8701 atcggatgaa gatggacgtc gatgttgtcg ccgggcagcc agcggccaaa cgtcgcgtac
     8761 ttcagcagcg cgtagaggat cgagttgccc tcgatcgcgc tgtccttcgg aatgggcccc
     8821 acgtcggaca gcgagagccc gacgagtagc aacggaattg cgacgacgag gcccgcaaca
     8881 ggtcccgccg cgccgacgtc gaacagcttc ttgcgatcgc ggatcggcgt gcgcatgcga
     8941 atgaccgcgc caagtgtgcc gagcgtcaga aacggcggca tcgggatgaa gtacggcaga
     9001 gagacatcct cgccatgcat gcgtccgacg acgtagtgac ctgcctcgtg acagagcaag
     9061 atgcccatca gggtggcgcc gaacgcgaac ccaccgacga tcgtcgtcgt gatgcaacag
     9121 gtgacaaaca acccgagaga gatcagcgcg tcgcgcccta gagacaccgg tagagtgtag
     9181 ggcatgggga cggtcaagtc agtccagctg ttcttccagg aggggacatc ggacaagctg
     9241 tacaacgccc agatcgtcga agacggtggc ggctacaccg tcgccgtgca gtggggacgc
     9301 cgcggtagct cgctgcagac cggctcgaag gccgtgaagg tctcgcgcgc gaaggcggaa
     9361 gcgacgttcg acaagatcgt tcgcgagaag cgcagcaaag gctacgaaga gatcggaggc
     9421 acggtgaaac cagccgcggt cgcgccgccc gccggctcgg ggtccgcatc acgagcgggt
     9481 gcaggcacca agcgcgcggt cgtcggtcac gcggcgcagc tgctcacccc gatcgaagac
     9541 gacgagctcg atcgcttcct ctctgacgag gatgtgatcg cgcaacagaa gatcgacggc
     9601 gtgcgcgtgc tcgtccacat cacgccggcg ggcgagctcg tcgcgacgaa ccggcacggc
     9661 cagcccacac agatggcggg cggcaatgcg ctcgcgggtc tcgcgtacct gccgaaggcg
     9721 acggtcgtcg atggcgagat cctcgacaac gcgtactgga tcttcgacgt actgcaagtc
     9781 gccggcaccg acgtccgcac gcacggctac ctcgatcgct ggaagctcct cgacgaagag
     9841 ctcgagccgg cgctcacggg caccgcgcag atcctgccga tggcgaaggg cgcgaaggcg
     9901 aagcgcacgc tgcacgacaa gctgcgccgc gtcggggccg aaggcatcgt gttcaagcag
     9961 cgcgatgcgc cgtacgcgca cggccgcgtg acgacccagc gcaagtacaa gttcatcaag
    10021 accgccgacg tgatcatcct cgagaacgtc ggcaacgcgt acaagatggc ggtgatggac
    10081 ggcgcgcgcc tgttcgaggt cggcaacgtc ttcgcgggga cgacgaacgc gtcgcgcaag
    10141 gatctcgacg cgcggctcgc acgcggtgag cgtccggtcg ctgaagttcg ctatctctac
    10201 gcgaccgatg accaccagct gtttcagccg gtgttcgtca cgctgcgcga cgacaagctc
    10261 gcgcgctcgt gcctgcgctc gcagctgctc gagacgagcc gccgcgtgat cgtctgattg
    10321 ccgctccgcg cgtgagcggt agccttctgt atgcgcgccc gcatcaaagt cggtacgacg
    10381 acgtgggcag gtcgcggcct cgtcgcaacg ggctggtacc cgcgcgacgt gaagacctcc
    10441 gaagaacgcc tgcactacta cgcatcgcag ttcccgatcg tcgagaacga ggccgggtac
    10501 tacgcgatcc tcacgcccga gcaggtcgcg ctgtgggcgg atcgcacgcc cgacggcttc
    10561 acgatgaaca tgaaggcgca cgcgctattt accgcgcact acgcagatcc tcgacggctg
    10621 ccgcgcgatg ttgtcgaggc gttgccgcgc ggcgtccgcg cgaaaccgca tgtctatccg
    10681 cgcgaccttt ccgtcgcgac gatgcgcgat ctcgagcggc gatttcgcga tgcgctgctg
    10741 ccgctacacg aggccggcaa gctcggcgtc gtactgtttc agttcccgat gtggttcgtg
    10801 atctcgcgcg agcacaaggc ggagctatcg cgcttgcgtg agtcgttcgc gccgtatcgc
    10861 gtcgcggtgg agctgcgcaa caagacgtgg ctgtccgagg agaatcgcga cgagacgctc
    10921 gcgcacttgc gcaaggagga catcgcctat tgttgcgtcg acgaggtcgg cccgatcccg
    10981 ccgatcgccg ccgcgacggc cgacatcgca gtcgttcgta tgcacgggcg gaacgcgtcg
    11041 cgcttcaagg gtcgccgttc gctgcccgag ggcgcgcgct acgtctacac gcaggccgag
    11101 ctgcgcgagt gggtgcctcg gatccgagag ctcgcaaagc acgcgcgcga agtacacgtt
    11161 ctcttcaaca acgcggtcgc ggactacgcc gttcgcggcg cgcacgatct cgtcgagctt
    11221 ctcgagggcg cgcgcggcgt gacgctcgca aaggccggct aaggcacgag cacgatctga
    11281 tcagcctgca cctcgagcaa tgccggctcg cgagcgagct cgccatcgcc atccatgctc
    11341 gcaggcccac acgcgccgtc gtcgagcttc gccctcgcga tcgcagtccg cagcgcacca
    11401 cgcggatcgg gcgacgtcat gcccaccgcc gcacgcgcct tcgcgatcaa cgtcgccgca
    11461 tcaaacgctt ctgcccccgc cgcgctcgga gtgcgattcg tcttctgctg aaacgcggcc
    11521 acgaagtccg ccgcgatctc gccgccatca ccaccggcaa acgcgtcgac gatgagcgcg
    11581 ccctgcaccg cctcgccgcc gcgcacgggc agcgacggat gatgccagcc ggcaccaccg
    11641 acgagctgca ccactgtcgg cacatgcccg ccatgcttgc gctgcagctg cgccgaattc
    11701 atgttctccg cggtccgcag ctcgacgccg tagtacggca gaaaggcggc gacaatcgcc
    11761 gcgcggtcat agcggtctgg aatgtagagc agcgagaacg gcacatccgg
//
LOCUS       DATLBZ010000306         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1089300, whole genome shotgun sequence.
ACCESSION   DATLBZ010000306 DATLBZ010000000
VERSION     DATLBZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089300"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VMZ53_15715"
     CDS             <1..810
                     /locus_tag="VMZ53_15715"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HUS29953.1"
                     /translation="DFIKRKLPSMYHQFKELAEVDITKDPMEVGPTLHYFMGGIRVDA
                     DSQMSTVPGLFACGECGAGLHGANRLGGNSLSDLLVFGKLAGNGAADYVRGLGSAPKS
                     PDEQILAGVRRATEVLKRDSGNNPYVLHDKLTETMQANVGIIREKAELEKALQDLEVL
                     KKDAETMKAPGTSQYNPGWHEALSMKSMLITAEAVARAALMREESRGAHTRNDFPGER
                     EEWVQYNVIIKKGANGMEVEKRKRPDPPPKLKEIAYAKIEDLESGKVGADA"
     gene            807..1598
                     /locus_tag="VMZ53_15720"
     CDS             807..1598
                     /locus_tag="VMZ53_15720"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HUS29954.1"
                     /translation="MSKRTFRIWRGSSEGGDFKTYEVEVDQGFVVLDVLHRIQADQAN
                     DLALRWNCKAGKCGSCSMEINGKPRLSCMTRMNIFAPDEVITVQPLKTFPVIKDLVTD
                     VSWNYQQNRRIRPFKPPQPRPEKGYVMYQEDVDRVQEFRKCIECYLCQDTCHVIRDRD
                     QKDSFVGPRFMIRLASLEMHPIDTDDRVPEIRKEFGSGFCNITRCCTEVCPEHINITD
                     NGIIPLKERVVDRYFDPVAIIGRKLFGIRRRTKTLAEEKGEKAEK"
     gene            1599..2099
                     /locus_tag="VMZ53_15725"
     CDS             1599..2099
                     /locus_tag="VMZ53_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29955.1"
                     /translation="MSGFELKRISADGIPHALEKAERYRLLNDPAQAESICRDILAVD
                     ADNQEALRVLILTLSDQFGAHGAGNAAREARNHIIQLKDEYERAYYTGIVFERETRAY
                     LSRKNVVRSAAYDGFRHAMEWYERAEQLRPPGNVDAVLRWNSCVRAIESERLEPESRE
                     PELPLE"
     gene            2138..>2403
                     /locus_tag="VMZ53_15730"
     CDS             2138..>2403
                     /locus_tag="VMZ53_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HUS29956.1"
                     /translation="MSRAPKASYDLVLLPGDGIGIEVIAEARALLDVIAPAIGVTFNA
                     DEIPCGGKFFLEHGSRDWPEGSEEKCAAADAILLGAVGWNGPDGA"
BASE COUNT          492 a          769 c          767 g          375 t
ORIGIN      
        1 gacttcatca agcgcaagct gccgtcgatg tatcaccagt tcaaggagct ggcggaagtc
       61 gacatcacga aggatccgat ggaagtcgga ccgacgctgc actacttcat gggcggcatt
      121 cgcgtcgatg cggactcgca gatgtcgacg gtgccgggcc tgttcgcgtg cggcgagtgc
      181 ggcgcgggtc tccacggtgc gaaccgcctc ggcggcaact cgctctccga cctcctcgtg
      241 ttcggcaagc tcgcgggcaa cggcgctgcc gactacgtgc gcggcctcgg ctcggcgccg
      301 aagtcacccg acgagcaaat cctcgcgggc gtgcgtcgcg cgaccgaagt gctcaagcgc
      361 gacagcggca acaacccgta cgtgctccac gacaagctca ccgagacgat gcaggcgaac
      421 gtcggcatca ttcgcgagaa ggccgagctc gagaaggcgc tccaggatct cgaggtcctc
      481 aagaaggacg ccgagacgat gaaggcgccg ggcacgagcc agtacaaccc cggctggcac
      541 gaggcgctgt cgatgaagtc gatgctcatc accgccgagg ccgtcgctcg cgcagcgctc
      601 atgcgtgaag agagccgcgg cgctcacacg cgcaacgact tcccgggcga gcgcgaagag
      661 tgggtgcagt acaacgtgat catcaagaag ggcgccaacg gcatggaagt cgagaagcgc
      721 aagcgtcccg atccgccgcc caagctcaaa gagatcgcgt acgcgaagat cgaagatctc
      781 gaatccggca aggttggagc tgacgcatga gcaaacgcac gttccgtatc tggcgtggca
      841 gcagcgaagg cggcgacttc aagacctacg aggtcgaagt cgatcagggc ttcgtcgtcc
      901 tcgacgtgct ccatcgcatc caagcggatc aggcgaacga cctcgcgctc cgctggaact
      961 gcaaggccgg caagtgcggc tcttgctcca tggagatcaa cggcaagccg cgcctgtcct
     1021 gcatgacgcg catgaacatc tttgcgccgg acgaggtcat caccgtccag ccgctgaaga
     1081 cgttcccggt gatcaaggac ctcgtcaccg acgtgtcgtg gaactaccag cagaaccgtc
     1141 gcattcgccc gttcaagccg ccccagccgc ggcctgaaaa gggctacgtg atgtaccaag
     1201 aggacgtcga tcgcgtccaa gagttccgca agtgcatcga gtgctacctg tgtcaggaca
     1261 cgtgtcacgt gatccgcgat cgcgaccaaa aggattcatt cgtcggcccg cgcttcatga
     1321 tccggctcgc gagcctcgag atgcatccga tcgataccga tgatcgcgtg cccgagatcc
     1381 gcaaagagtt cgggtcgggc ttctgcaaca tcacgcggtg ctgcacggaa gtctgccccg
     1441 agcacatcaa catcacggac aacggcatca tcccgctcaa agagcgcgtc gtcgatcgct
     1501 acttcgatcc ggtcgcgatc atcggtcgca agctgtttgg catccgccgt cgcacgaaga
     1561 cgctcgccga agaaaaaggc gagaaggccg aaaagtagtt gagcggtttc gagctgaagc
     1621 ggatctcggc cgatgggatc ccgcacgcac tcgaaaaagc agagcgctat cgcctgctca
     1681 atgatccggc gcaggccgaa tcgatctgcc gcgacatcct cgcggtcgac gccgacaacc
     1741 aagaagcgct gcgcgtcctg atcctgacgc tctccgatca gttcggcgcg cacggcgccg
     1801 gcaatgcagc acgcgaagcg cgcaatcaca tcatccagct caaagacgaa tacgagcgcg
     1861 cgtactacac gggcatcgtg ttcgagcgcg agacgcgcgc atacctgtcg cgcaagaacg
     1921 tcgtgcggtc ggctgcgtac gacggctttc gccacgcgat ggagtggtac gagcgcgccg
     1981 agcagcttcg cccgcccggc aacgtcgatg cggtgctgcg ctggaacagc tgcgtgcgcg
     2041 cgatcgagag cgagcggctc gagccggaat cgcgcgagcc cgagctgccg ctcgagtagc
     2101 catcaccgcg gcgacgagcg gtgctacgct gcgcgcgatg tcgcgtgctc cgaaggcctc
     2161 gtacgacctc gttctcctgc ccggcgatgg aatcggcatc gaggtgatcg ccgaagcgag
     2221 agcgctgctc gatgtgatcg cgcccgcgat cggcgtgacg ttcaacgccg acgagattcc
     2281 gtgcggcggg aagttcttcc tcgagcacgg ctcgcgcgat tggccggaag gctccgaaga
     2341 gaagtgcgca gctgccgacg cgatcttgct cggcgcggtc ggctggaacg gtcccgacgg
     2401 tgc
//
LOCUS       DATLBZ010000307        10558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3162573, whole genome shotgun sequence.
ACCESSION   DATLBZ010000307 DATLBZ010000000
VERSION     DATLBZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3162573"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VMZ53_15735"
     CDS             <1..615
                     /locus_tag="VMZ53_15735"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUS29957.1"
                     /translation="GHPLLTAARAGELSTKTLHEFAFHQYSDSILWIPMLAQMKSAAR
                     KSRRLREAIEANIGHEAGLQGTCHVTLAVQFMRSLGITELDDMPTETFTREATEWLSP
                     DFQVLSEPVVAGWLLVAESLVPLMFAALEPSFAALGADTRYLREHVHVDSDEHARWMA
                     ESVEEIVELYGPACLAEIREGMVDGWEETVRVPDALWRSQCVSV"
     gene            600..1607
                     /locus_tag="VMZ53_15740"
     CDS             600..1607
                     /locus_tag="VMZ53_15740"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29958.1"
                     /translation="MRIGLTFNQKRSGCETQAEFDTLETVHAIARMLASLGHVVTPIE
                     VTRPVDEIVGELRRIAPELVFNMAEGTRGRFREALLPALCEQLGIAHTGSSASVLALC
                     MDKALAKRVVAGARVRTPWSRLVRSTSDPLPPIGGPVIVKPNFEGSSKGITQESVVAD
                     PAKIGDVVRAQLARYPNGILVEEYIDGTDVAVGWVEGFGVLPPIGYSYSASGPHRIYD
                     LGLKAGPAEDVVLEIPAALSPAVAAALRTAATRAFEALGVAGYGRADFRVTGDGRVFF
                     LEMNPLPTLDPDDRELYAAAAARGHSARDLLAAIVASASCAMEPAQELETQVPLHSPA
                     A"
     gene            complement(1540..4455)
                     /locus_tag="VMZ53_15745"
     CDS             complement(1540..4455)
                     /locus_tag="VMZ53_15745"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HUS29959.1"
                     /translation="MKRLLSLVLVASVACGGSSKPAPGPTRPPDFAPPPTDGATSTSP
                     APASSDNAPLPLWPEVKKGTLSNGLTYYILRHGKPEKRALLWLAVNAGSVQEDDDQRG
                     LAHFDEHMAFNGTKRFPKNDIVKYLERIGMRFGADLNAYTTWDQTVYQLEVPSDNADF
                     IAKGLDILRDWSTDVQYDAGEVDKERGVVKEEWRLGRGAGARLFDKHSKVLFKGSRYA
                     DRLTIGLPEILDKAPRDTLYRYYKDWYRPDLMAVIAVGDFDVAQMESEIKARFGDLKA
                     ATKPRPRPQGGVPPAEGTRVSIETDREATGTTVSIQNLIAHRPEASKRDFRRLLAEQA
                     YSAVLNERLSSLQRKPDAPFLNAAGGIAQSLRTVDSFSRFASAKQGKLEEALRVLLTE
                     VVRIEKHGITQTELDRALADMKRSAEQAAETADTRDSRRFTDEITRNFFEAEEMVGPK
                     AERDMTLEILPTLTLAELNKLGASFGGAEGRVILISGPEGKPLPSKERVLAIVDEVNK
                     SKVDPFEDSVPQTALMDKTPAPGKITKESQVDKIGVTEWTLSNGVKVVVKPTDFEIDS
                     VSISGISPGGEAMASDKDYPNARWADEVAGLGGVGEFDVDMLAKVLAGKQVRASASIG
                     EVSEAIDAGGSVKDLETMFQLIHLRMTAPRKDEQAFGVWRSNFAEQITNALRSPEFRY
                     ARESQLALYKGHLRRKPAEPKDIVSIDQDKALAFYKNRFGDASDFTFVIVGAVKLDEL
                     KPLVETYLASLPAKGRKEKEKDLKIRKIGGAVKKEFKLGSEPKASVQIDFHGDYKWTR
                     DNDRDMFILGQVSSILLREIMREDKGGVYGVGAGGRISRSPYQERTFGVRFGCDPARV
                     NELVKVVFDNAESMKKEDVPADALERVKQTFLRTRETDLRRNGFWVGWLATAYRYGDD
                     PTIILDTEAVTKRMTAANVKASAKKFFDGKSYYEAVMLPENAAAPASPAPAPAPSHN"
     gene            complement(4533..5786)
                     /locus_tag="VMZ53_15750"
     CDS             complement(4533..5786)
                     /locus_tag="VMZ53_15750"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUS29960.1"
                     /translation="MQAPSTRPKIGFVLSGGGSRGAYEAGIIHYLRTDLAKRLGRQVP
                     IDIVTGTSVGAINAAYLAATMDQPENQAHTLTAAWRSLRIEELISLRAKDMMRAVKML
                     LGRDPPPPAPGSFRYGGLLDTSGLERFVIRAIPWRGIEKNLRNRSLHAMSVSATHVGS
                     GHTVVFLSSAEPVPREWSRDPFVRHRAARIGPRHVLASAAIPMLFPAVKIGEEFFTDG
                     GLRQNTPMSPAIRLGADRLLLVSLKHVKNEPKVVEKDRVEAYPKPLFLAGKALNALML
                     DHTEYDLMRMQRINMILEAGNASFGPRFEEMMNHELVRLRGAPLRRIQTVHIRPSEDI
                     GRIAADFASSGKVKVSGPIAKGLIHRLAAGEARRESDLLSYLLFDGDFAAELIDLGRQ
                     DAAKKEDELAALFDVMSPAAAATGA"
     gene            5848..6282
                     /locus_tag="VMZ53_15755"
     CDS             5848..6282
                     /locus_tag="VMZ53_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29961.1"
                     /translation="MRHLLLILSLVATACAGSPPRETTPPKKFAMKTYTFVLLKRGPA
                     WSPEETPESKKLFEGHMANIKAMARARKLIIAGPFDAPKDDKAAYAGLFVFDVPAEEA
                     KQLLAGDPAIVAGRLVPEMMPWYGPAGLTYDGAEEALREPPH"
     gene            complement(6290..7114)
                     /locus_tag="VMZ53_15760"
     CDS             complement(6290..7114)
                     /locus_tag="VMZ53_15760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS29962.1"
                     /translation="MTKTFELETGTLHALELGAGKPLLFLHGFPDHPPTAQRFFDELA
                     SRGFRVLAPWLPGYAPSPTDGPMTTSRVARDLLALLESWSPGAPVDIVGHDWGAVMTY
                     VLCASAPDRFHRAVTMAVPHPRTFARQLRTAKQLRASWYMAFFQLPGSARVTAARDFA
                     LVDRLWRTWSPTFALPDDQRAALHACLAQSMPAPLRWYRAAARDRSGMQLFRSPITTP
                     LLYLHGADDGCIVPPTVDDSRRFTEYERAVLEDLGHFLHLEDPAGVAERVATWLAH"
     gene            complement(7138..8940)
                     /locus_tag="VMZ53_15765"
     CDS             complement(7138..8940)
                     /locus_tag="VMZ53_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HUS29963.1"
                     /translation="MSAPYVDPPDEQDRPRDRRKLAVLWRLTVLMRPYAARFFFAVFM
                     LFGASALTLVYPLAAKYAVDIGMKQQTTTTLDTFVLGMIVLFILNAGFVWLRHYSLSW
                     LGERVVTDLRGLVFDRLLTLPLNWFHERRSGELVGRLASDVTVIEGVVGSDLSLALRN
                     GIQIVGALVLLFIIDVKLTMLMLAIVPPIVLTTLFFGRKIRRMARGVQDELAKVSGQA
                     QESVGAIATVQAFAREGYEAKRYRSGVEAAFNKTLEMVRLRASFFATSMSAGYAGVAA
                     VIWLGGRALIHKELTAGDLTSFFLYTFIVAGALADLASLWANLQRAAGATDRLFAVID
                     TLPDIRDREDAKPLPQGGGEITLEKVSFSYPTRRAQPVLDNVDLEIKPGEVVALVGPS
                     GAGKSTILSLLYRFYDVDDGRVLFEGVDVRELKLAELRRSLAIVAQEPVLWAGSIRDN
                     IAYGREGVTDAEIEAAARDAFAHDFITGFPDGYKTIIGERGTKLSGGQKQRIALARAL
                     VANPRVLILDEATSNLDAESEAAVQAALHRVRQSRTTIIVAHRLSTVRDADRIIVIEG
                     ARVVEQGAHDVLMERRGTYRRLVEHQLIADRAAS"
     gene            8980..>10558
                     /locus_tag="VMZ53_15770"
     CDS             8980..>10558
                     /locus_tag="VMZ53_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29964.1"
                     /translation="MRSLPLLALAVGVVATGCRNKSKLDVDRGADVDALWDLAPDDTQ
                     LGLVASPRAIGLAIRAVNAIRDISAQPDLAPAKPQIDMLVKGLYGSADGTAADAGFSD
                     SRGFAMFATTDGVLGVMPVVDRDKFMATKHGSRGTLGSGGDDALEGNTCREIRGLYVC
                     ASKVEMFERLGKGSLRGKLDTGPGHARGDAELYMKRATLLGDAEGVLSVAAILDVGTV
                     DVHARWNGTPSTPLADLINIVAPHPNTDSSSGFVTFDAKPLFSAVPPLPIAGGVTLDQ
                     LTASLVGPITAVIPSGSVDIQVHAPLSDPKPAQTIVEHCTDIGNFFELAKTQTPGACR
                     IVLQGTTQLELDIWVDNNELRLGAHKGAAPQGKRGGMTSTGSALAASDWTAAFWGRGT
                     MLNLQGIAPATADVPAEVALGIHAIALINELGAAAKVDPTGVTFRAYLRTAWTNPPDV
                     LAKIAAITGNEIVTGKATEPAAAIATATPSAPFAADFAAGQGGLMIPAAAIGVAAAVV
                     IPAILQAVSNTRGDAQAA"
BASE COUNT         1760 a         3500 c         3538 g         1760 t
ORIGIN      
        1 ggccatccac tgctgaccgc ggcgcgagct ggagagctct cgaccaagac tctgcacgag
       61 ttcgcatttc accagtactc agacagcatc ctgtggatcc cgatgctggc gcagatgaag
      121 agcgccgctc gcaaatcgcg ccggttgcgc gaagcgatcg aagcgaacat cggtcatgaa
      181 gcggggctcc agggcacgtg ccacgtgacg ctcgccgtcc agttcatgcg cagcctcggg
      241 atcaccgagc tcgatgacat gccgacggag acgttcacgc gcgaggcgac cgaatggttg
      301 tcgcctgact ttcaagttct gagcgagccg gtcgttgcgg gctggctgct cgtcgccgaa
      361 tcgctcgtcc ctctcatgtt cgcggcgctg gagccgagct ttgccgctct tggcgccgat
      421 acgcgctacc tgcgcgagca cgtccacgtc gacagcgacg agcacgcgag gtggatggcg
      481 gaatccgtcg aagagatcgt cgagctctac ggtcctgcat gcctcgccga gattcgcgag
      541 ggcatggtcg atggctggga agagaccgtt cgggttccgg acgcactctg gaggtcgcaa
      601 tgcgtatcgg tctgaccttc aaccagaagc gctccggctg cgagacgcag gccgagttcg
      661 acacgctcga gaccgtgcac gcgatcgcgc gcatgctggc gagcctaggc cacgtagtca
      721 caccgatcga ggtcacgcgt ccggtcgacg agatcgtcgg cgagcttcgc cggatcgcgc
      781 ccgagctcgt gttcaacatg gccgagggca cgcgcggaag gttccgtgaa gcgctgctgc
      841 cggcgttgtg cgagcagctc ggcatcgcgc acaccggaag ctcggcgagc gtgctcgcgc
      901 tgtgcatgga caaggcactc gcgaagcgcg tcgtcgcggg tgctcgcgtg cggacgccgt
      961 ggagccgcct cgtgcggtcg acgagcgatc cgttgccgcc gatcgggggc cccgtgatcg
     1021 tgaagccgaa cttcgagggt tcgagcaagg gcatcacgca agagagcgtc gtcgcggatc
     1081 ccgcaaagat cggcgacgtc gtgcgggccc agctcgcgcg ttacccgaat ggcatcctcg
     1141 tcgaggaata catcgacgga accgacgtgg cggtcggctg ggtcgaaggc ttcggcgtgc
     1201 tgcctccgat cggctactcg tattcggcga gtgggccaca ccggatctac gacctcggtc
     1261 tcaaggcagg gcccgccgag gacgtcgttc tcgagatccc ggcagcgctg tcaccggcgg
     1321 tagcggccgc actgcggacc gcagctactc gagcgttcga agcgctcggc gtcgccggtt
     1381 acggccgtgc agacttccgg gtcaccggag acggccgcgt attcttcctc gagatgaatc
     1441 cgctgccgac gctcgatccg gacgatcgcg agctctacgc cgcggcggca gctcgcgggc
     1501 acagcgctcg tgatctgctc gccgcgatcg tggcgtccgc tagttgtgcg atggagccgg
     1561 cgcaggagct ggagacgcag gtgccgctgc attctccggc agcatgacgg cctcgtagta
     1621 cgacttcccg tcgaagaact tcttcgccga ggccttcacg ttcgcggcgg tcatcctctt
     1681 cgtcaccgct tcggtgtcga ggatgatcgt cgggtcgtcg ccgtaacggt atgcggtcgc
     1741 cagccaaccg acccagaagc cgttgcggcg aagatccgtc tcgcgcgtgc gaaggaacgt
     1801 ctgcttgacg cgctcgaggg cgtccgcagg gacgtcttcc ttcttcatgc tctccgcgtt
     1861 gtcgaacacg accttgacga gctcgttaac gcgcgccgga tcgcagccga agcggacgcc
     1921 gaacgtgcgc tcctgatacg gcgaccgcga gatccggccg cctgcgccga cgccgtacac
     1981 gccgcccttg tcctcacgca tgatctcgcg cagcaggatc gatgacacct ggccgaggat
     2041 gaacatgtcg cggtcgttgt cacgcgtcca cttgtagtca ccgtggaagt cgatctggac
     2101 cgacgccttt ggctcgctgc cgagcttgaa ctccttcttc accgcgccgc cgatcttgcg
     2161 gatcttgaga tccttctcct tctctttgcg gcctttcgcc gggaggctgg cgaggtacgt
     2221 ctcgacgagg ggcttcagct cgtcgagctt cacggcgccg acgatgacga acgtgaagtc
     2281 cgatgcgtcg ccaaaccggt tcttgtagaa cgcgagggcc ttgtcctgat cgatcgacac
     2341 gatgtctttc ggctcggccg gcttgcggcg gaggtggccc ttgtacagcg cgagctgtga
     2401 ctcgcgtgcg tagcggaact ccggcgaccg cagcgcattc gtgatctgct cggcgaagtt
     2461 actgcgccag acgccgaacg cttgctcgtc cttgcgcggc gcggtcatcc gcaggtggat
     2521 gagctggaac atcgtctcga ggtccttcac cgagccaccg gcgtcgatcg cctcggagac
     2581 ctcaccgatg cttgccgagg cgcgcacctg cttgccggcg aggacctttg cgagcatgtc
     2641 gacatcgaat tcgccgacgc cgccgaggcc cgcgacctca tcggcccagc gcgcgttcgg
     2701 gtagtccttg tccgatgcca tcgcttcgcc gccgggcgag atgccgctga tcgacaccga
     2761 gtcgatttcg aagtcggtcg gcttcacgac gaccttcacg ccgttcgaca gcgtccattc
     2821 ggtgacgccg atcttgtcga cttgcgactc cttcgtgatc ttgcctggcg ccggcgtctt
     2881 gtccatgagc gccgtctgcg gaacggagtc ctcgaacgga tcgaccttcg acttgttcac
     2941 ttcgtcgacg atcgcgagca cgcgctcctt cgacggcagc ggcttgccct cgggacccga
     3001 gatgagaatg acgcggcctt cggcgccgcc gaacgaggca ccgagcttgt tgagctccgc
     3061 gagcgtgagc gtcggcagga tctcgagcgt catatcgcgc tcggccttcg ggccgaccat
     3121 ctcttcggct tcgaagaagt tgcgcgtgat ctcgtcggtg aacctgcggc tgtcgcgcgt
     3181 atcggcggtc tccgccgctt gctccgcgct gcgcttcatg tcggcgagcg cacggtcgag
     3241 ctcggtctgc gtgatgccat gcttctcgat gcgcacgacc tccgtgagca ggacgcgcag
     3301 cgcctcctcc agctttcctt gcttcgcact cgcgaaccgc gagaacgagt cgacggtgcg
     3361 cagcgactgc gcgatgccgc cagctgcatt gaggaacggc gcgtccggct tgcgctgcag
     3421 agagctcaag cgctcgttca gcaccgcgga gtacgcctgc tccgcgagca gacgacggaa
     3481 gtccctcttg ctcgcttcgg ggcgatgcgc gatgaggttc tggatcgaca ccgtcgtgcc
     3541 ggttgcttca cgatcggtct cgatcgagac gcgcgttccc tcggcgggcg gcactcctcc
     3601 ctgcgggcgc ggacgcggct tcgtcgcggc cttgagatcg ccgaaccgcg ctttgatctc
     3661 gctctccatc tgcgcgacat cgaaatcacc gacggcgatc accgccatca ggtcagggcg
     3721 ataccagtcc ttgtagtagc gatagagcgt gtcgcgcggg gccttgtcga ggatctcggg
     3781 aaggccgatc gtcaggcggt ccgcgtaacg cgagcccttg aacagaacct tcgagtgctt
     3841 gtcgaacaga cgcgcgccgg ctccgcgccc gagccgccat tcctctttga cgacgccgcg
     3901 ctctttatca acctcgcccg cgtcgtactg cacgtcggtc gaccagtcgc gaaggatgtc
     3961 gaggcccttc gcgataaagt cggcgttgtc gctcggaact tcgagctgat agaccgtctg
     4021 atcccacgtc gtgtacgcat tgagatcggc gccgaaccgc atgccgatgc gctcgaggta
     4081 cttgacgatg tcgttcttcg gaaaccgctt cgtcccgttg aacgccatgt gctcgtcgaa
     4141 gtgggcgagc ccgcgctggt cgtcgtcctc ttgcaccgag ccggcgttca ccgcgagcca
     4201 caacagcgca cgcttctcgg gcttgccgtg gcgaaggatg tagtacgtga gcccgttcga
     4261 cagcgtgccc ttcttgacct ccggccacag agggagcggc gcattgtcgg agctcgccgg
     4321 cgcggggctc gtgctggtcg cgccgtccgt cggaggtggc gcaaaatccg gcgggcgcgt
     4381 cggcccgggc gcaggcttgg aggagccgcc gcaggcgacg cttgcgacga gaacgagcga
     4441 gagcagacgc ttcatgccgc ggacattgcc acggcaaccg cactaccgca gcgcaaacag
     4501 cacgcgataa ccgtgcgacg cgtgcgacac cgtcacgcgc cggtcgcggc cgctgcgggc
     4561 gacatcacat cgaagagcgc ggccagctcg tcttccttct tcgcggcatc ttgccggccg
     4621 aggtcgatga gctccgccgc gaaatcgcca tcgaatagaa gataggacag caggtcgctc
     4681 tcgcgtcgcg cttcgccggc cgccaaccga tggatcaatc ccttcgcgat cgggccggaa
     4741 accttcacct tgccagagga cgcgaagtcc gcggcgatgc gcccgatgtc ttccgacgga
     4801 cgaatgtgca ccgtctgaat gcgccgcagc ggcgcgccgc gcagccgcac gagctcgtgg
     4861 ttcatcatct cctcgaagcg cgggccgaac gacgcgttgc ccgcctcgag gatcatgttg
     4921 atccgctgca tgcgcatgag gtcgtactcg gtgtgatcga gcatcagggc gttgagcgcc
     4981 ttgccggcca agaacagcgg cttgggatat gcctcgacgc ggtctttctc gacgaccttc
     5041 ggctcgttct tcacgtgctt gagcgagacg agcaacagcc ggtccgctcc gagccggatc
     5101 gcgggcgaca tcggcgtgtt ctgccgcaag ccaccgtcgg tgaagaactc ctcgccgatc
     5161 ttcacggccg gaaacagcat cgggatcgcc gccgaggcga gcacgtgccg cggaccgatc
     5221 cgcgccgcgc gatgccgcac gaacggatcg cgactccact cgcgaggcac aggctccgcg
     5281 ctcgacagaa acacgaccgt atggccgcta cccacgtggg tcgccgacac gctcatcgca
     5341 tgcagcgagc gattgcgtag gttcttctcg ataccgcgcc aaggaatcgc gcgaatgacg
     5401 aaccgctcga ggccgctcgt atcgaggagc ccgccatagc ggaagctgcc cggtgccggc
     5461 ggcggcggat ctctgccgag cagcatcttc acggcgcgca tcatgtcctt cgcgcgaagg
     5521 ctgatgagct cctcgatgcg caacgaccgc cacgccgcgg tgagcgtgtg ggcctgattc
     5581 tccggctggt ccatcgtcgc ggccagatac gccgcgttga tcgcgccgac gctcgtgccg
     5641 gtcacgatgt cgatcgggac ctgccggcca aggcgcttgg ccaagtccgt acggaggtag
     5701 tgaatgatgc cggcctcgta cgcaccgcgc gacccgccgc cagagagcac gaagccgatc
     5761 ttgggtcggg tcgaaggcgc ctgcaccgat ctccattgtc gcgttgcccg cgctcccggc
     5821 gcaactcccg acatacgatg cgcgccgatg cgccatttgc tgctgatcct gagccttgtt
     5881 gctaccgcgt gcgccgggtc acctccccgt gaaaccacgc ccccgaagaa gttcgcgatg
     5941 aagacctaca cgttcgtgct gctgaagcgc ggcccggcct ggagccccga agagaccccc
     6001 gaatcgaaga agctgttcga gggccacatg gcgaacatca aggcgatggc gcgcgcgagg
     6061 aagctgatca tcgccggacc attcgacgcg ccgaaagacg acaaggcggc ctacgccggc
     6121 ctgttcgtct tcgatgtccc cgccgaagaa gcaaagcagc tgctcgcggg tgatccggcg
     6181 atcgtggcgg gccgcctcgt tcccgagatg atgccgtggt acggcccggc cggactgacg
     6241 tacgacggcg ctgaggaagc gctgcgcgag ccgccgcact gatgcgcgat tagtgtgcga
     6301 gccaggtcgc gacgcgctct gcgacgccgg ctggatcttc gagatgcagg aagtgcccga
     6361 ggtcctcgag gaccgcgcgc tcgtactccg tgaagcggcg cgagtcgtcg accgtcggcg
     6421 gcacgatgca gccatcgtcg gccccgtgga gatagagcag cggcgtcgtg atcggtgagc
     6481 ggaacagctg catgccgctg cgatcgcgag cggcggcgcg ataccagcgc agcggcgcgg
     6541 gcatgctctg cgcgaggcac gcgtggagag cggcgcgctg gtcgtcgggt agcgcgaagg
     6601 tcggcgacca cgtgcgccac aggcgatcga cgagcgcgaa gtcgcgcgct gccgtgacgc
     6661 gggcacttcc cggcagctgg aagaacgcca tgtaccagct cgcgcgcagc tgcttcgcgg
     6721 tgcggagctg ccgcgcgaat gtgcgcggat gcggaacggc catcgtgacg gcgcgatgga
     6781 agcgatcggg cgcgctcgcg cagagcacgt aggtcatcac cgcgccccag tcgtggccga
     6841 cgatgtcgac gggtgcgccg ggcgaccacg actcgagaag cgcgaggaga tcgcgcgcga
     6901 cgcggctcgt cgtcatcgga ccgtcggtgg gcgagggcgc gtagccgggg agccagggcg
     6961 cgagaacacg gaagccgcgg gatgcgagct cgtcgaagaa gcgctgggcg gtcggcgggt
     7021 ggtcggggaa gccgtggagg aacagcaacg gtttgccggc gccgagctcg agcgcgtgga
     7081 gcgtgcctgt ctcgagctcg aacgtcttgg tcatgggcac gaaggtcgtg tcgatgttca
     7141 cgaggccgcg cggtcggcga tcagttggtg ctcgacgagg cgacggtacg taccgcggcg
     7201 ttccatgagg acgtcgtggg cgccttgctc gacgacgcgc gcgccttcga tgacgatgat
     7261 gcggtccgca tcccggacgg tcgacaggcg gtgcgcgacg atgatcgtcg tgcgcgattg
     7321 gcggacacgg tgcagcgctg cttggacggc ggcttcactc tcggcgtcga ggttcgatgt
     7381 cgcttcgtcg aggatgagga cgcgcgggtt cgcgacgagc gcgcgggcga gcgcgatgcg
     7441 ctgcttctgg ccgccggaca gcttcgtgcc gcgctcgccg atgatggtct tgtagccgtc
     7501 ggggaagccg gtgatgaagt cgtgggcgaa tgcgtcgcgt gcggcagctt cgatctcggc
     7561 gtcggtgaca ccttcgcggc cgtacgcgat gttgtcgcgg atcgagccgg cccagagcac
     7621 gggctcttgc gcgacgatcg cgagcgagcg gcgcagctcg gcgagcttga gctcgcgcac
     7681 gtcgacgccc tcgaagagga cgcgtccatc gtcgacatcg tagaagcggt agagcagcga
     7741 gaggatcgtc gacttgccgg cgcccgacgg accgacgagc gcgacaactt cgccgggctt
     7801 gatctcgagg tcgacgttgt cgagcacggg ctgcgcacgg cgcgtcggat acgagaacga
     7861 gactttttcg agcgtgattt caccgccgcc ttggggcagc ggcttggcgt cctcgcgatc
     7921 gcggatgtcg ggcagcgtat cgatcaccgc gaacaagcgg tcggtggcgc ccgcggcgcg
     7981 ctggaggttg gcccacaggc tggcgaggtc agcgagcgcg ccggcgacga tgaacgtgta
     8041 gaggaagaac gacgtcagat cgccggcggt gagctccttg tgaatgagcg cgcgaccgcc
     8101 gagccagatc accgcagcga cacccgcgta gcccgccgac atcgacgttg cgaagaatga
     8161 cgcgcggagc cgcaccatct cgagcgtctt gttgaatgcg gcctcgacgc cggagcgata
     8221 gcgcttcgct tcgtagccct cgcgcgcgaa ggcctggacg gtggcgatcg cgccgaccga
     8281 ttcctgcgct tggccggaga ccttggcgag ctcgtcttgg acgccgcggg ccatgcgccg
     8341 gatcttgcgg ccgaagaaca gcgtcgtcag gacgatcggc ggcacgatcg cgagcatgag
     8401 catcgtcagc ttcacgtcga tgatgaacaa cagcacgagc gcgccgacaa tctggatccc
     8461 gttgcgcagt gcgagcgaga ggtcactacc gacgacgcct tcgatcaccg tcacgtcact
     8521 cgcgagacgg ccgacaagct cgccgctgcg tcgttcgtga aaccagttga gcggcagcgt
     8581 gagcaatcga tcgaacacga ggccgcgcaa atcggtgaca acgcgctccc cgagccacga
     8641 cagcgagtag tggcgcagcc acacgaagcc cgcgttcagg atgaacagca cgatcatgcc
     8701 gagcacgaat gtgtcgagcg tggtggtcgt ctgctgcttc atcccgatgt cgaccgcgta
     8761 cttggcggcc aacggataga cgagcgtcag cgctgacgcg ccgaacagca tgaagacggc
     8821 gaagaagaag cgcgcggcgt acggccgcat gagcacggtc agccgccaga gcaccgccag
     8881 cttgcggcga tcgcgcggcc gatcctgctc gtcgggagga tcgacgtacg gcgcgctcac
     8941 cgatggtgca tagcatggtt accgagctag aaccgggcca tgcgttcgtt gccgctcctc
     9001 gcccttgccg tcggtgtcgt cgccaccggc tgccgcaaca agtcgaagct cgatgtcgat
     9061 cgcggcgcgg atgtcgacgc cctctgggac ctcgcgcccg acgacacgca gctcggcctc
     9121 gtcgcatcgc cgcgtgcgat cggcctggcc attcgggcag tcaatgcgat tcgcgacatc
     9181 tcggcgcagc ccgacctcgc gccggccaag ccgcagatcg acatgctcgt caaaggtctc
     9241 tacggcagcg cggacgggac ggcggccgac gctggcttct ccgactcgcg cggcttcgcg
     9301 atgttcgcga cgacagacgg cgtgctcggc gtgatgcccg tcgtcgatcg cgacaagttc
     9361 atggcgacga agcacggctc gcgcggcacg ctcggctcgg gcggcgacga cgcactcgaa
     9421 ggcaacacgt gtcgcgagat ccgcggcctc tacgtctgcg cgtcgaaggt cgagatgttc
     9481 gagcgcctcg gcaaaggctc gctgcgcggc aagctcgata ccggtccagg ccacgcgcgc
     9541 ggtgacgcag agctctacat gaagcgagcg actctgttgg gtgatgccga aggcgtgctc
     9601 agcgtggcgg cgatcctcga cgtcggcacc gtggacgtac acgcccggtg gaacggcacg
     9661 ccgtcgacgc cgctcgcgga tctcatcaac atcgtcgcgc cgcaccccaa cacagacagc
     9721 tcgtcgggct tcgtcacgtt cgacgcaaag ccgctcttct cggccgttcc tcctctaccg
     9781 atcgcaggcg gcgtcacgct cgatcagctc accgcatcgc tcgtcggtcc gatcacggcc
     9841 gtgatcccgt cgggctccgt cgatatccaa gtccacgcgc cgctgagcga ccccaagcct
     9901 gcacagacga tcgtcgagca ctgcacggac atcggcaatt tcttcgagct cgcaaagact
     9961 cagacgccag gcgcgtgccg catcgtgctc caaggcacga cgcagctcga gctcgacatc
    10021 tgggtcgaca acaacgagct tcgcctcggc gcgcacaagg gcgcagctcc gcaaggcaag
    10081 cgcggcggca tgacgtcaac gggctcggcg ctcgctgcgt ccgactggac cgctgcgttc
    10141 tggggccgcg gcacgatgct gaacctccag ggaatcgcgc ccgccaccgc ggacgtcccc
    10201 gcagaagtcg cgctcggcat tcatgcgatc gcgctcatca acgagctcgg tgcagccgcg
    10261 aaagtcgacc ccaccggcgt gacgttccgc gcgtacctgc gcacggcgtg gacaaacccg
    10321 cccgatgtcc tcgcgaagat cgccgcgatc accggcaacg agatcgtgac gggcaaggcg
    10381 accgagcccg cggccgcgat cgccaccgcc actccatcag cgccattcgc cgcagacttc
    10441 gcagcgggcc aaggcggcct gatgatccca gctgccgcga tcggcgtggc cgcagcggtc
    10501 gtcatcccgg ccatcctgca agccgtctcg aatacgcgcg gcgacgcgca agcagcca
//
LOCUS       DATLBZ010000308         2972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4812468, whole genome shotgun sequence.
ACCESSION   DATLBZ010000308 DATLBZ010000000
VERSION     DATLBZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4812468"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VMZ53_15775"
     CDS             complement(<1..481)
                     /locus_tag="VMZ53_15775"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HUS29965.1"
                     /translation="MAVAVLTRDSVDAQAYAAALVPLGLEAVAMPVTKPAPAADPDAL
                     ARAVGHGSYDVIMVASPRAAHELARAVAASVPTRVTMPDLPDVYAVGPATKRALDIAR
                     LPGQVPANVRDGVELAKWIAAKMTLKGKRVLVPRADEGRLDGIAILREAGADVVDVVA
                     "
     gene            616..1014
                     /locus_tag="VMZ53_15780"
     CDS             616..1014
                     /locus_tag="VMZ53_15780"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HUS29966.1"
                     /translation="MATVARVLDNYMIDPLIGLVIPGGGDVIGSLLGVYTVMIAVRRK
                     VSPVIIARMIMNLGLDAVMGFIPFIGDLVDFGFKANQKNVALLSQSLETRHGKASAKD
                     WLVVAGAAAAYIAIMAFVVWGIVKIFQAIF"
     gene            complement(1025..1519)
                     /locus_tag="VMZ53_15785"
     CDS             complement(1025..1519)
                     /locus_tag="VMZ53_15785"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HUS29967.1"
                     /translation="MRFVVIAVLACVACQNEAKRQDDMPPKPSGSSTQTQPSGPRPTV
                     YIQSATGDVAVTVEVVATETKIERGLMYREHLGLDAGMLFMMGKEKPWSFWMRNTLIP
                     LDMLFIGRDKTVVGIVENAEPKTETLRQVEKPSLYVLEVNGGWSKQHQVAAGAKVRFE
                     NVPE"
     gene            complement(1536..>2972)
                     /locus_tag="VMZ53_15790"
     CDS             complement(1536..>2972)
                     /locus_tag="VMZ53_15790"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS29968.1"
                     /translation="NFKDQGAARDFSQWRLRGFPQELTDGDGHFTLTGLAPGSYMVRA
                     TRSQNASRGRRDAIEGTVAETGTNNLKLVLQPEGAVKGKVQLADGSAPGAFTIQVGFT
                     QQAFTGTSGEFLLDALPPQKYELSVRGPSFQTKAVEVTVDPGKTADAGTISVTKGRIL
                     AGVVVAEGQPVANATVYAGRQIFGSGSTNSANFGPMGQGTKTDTTDASGKFVISGLNE
                     GDIAVVAEQPTIGRSKAMRVSTAMPGQGELVLELQKFGSLSGILRVAGKPAEGVFVSC
                     QSTTTPGAIYAVASGPDGAYRYDRLAPDTYKVSATVGMPMMGMKFYSKEIVVPPSKDV
                     TLDLSADPGALTLAVNAVGKGGAKIGVASAFLATGVVAARTMTDLSLQMAAAGASSSQ
                     WVIIRNGEPAKFTEVAPGTYSACVVPFPVEVQGMAAMGYAERHSDSMRAFCQSIKVNP
                     APPEQSMQVAVEPPPFINDNAGSGSGAP"
BASE COUNT          470 a          989 c          965 g          548 t
ORIGIN      
        1 acgcgacaac gtcgacaacg tcggcgcctg cctcgcgcaa gatcgcgatg ccgtcgagcc
       61 gtccctcgtc cgcacgcggc acgagcactc gcttgccctt cagcgtcatc ttcgcggcaa
      121 tccacttcgc gagctccacg ccatcgcgga cgttcgccgg tacctgccct gggagcctcg
      181 caatatcgag cgcgcgtttc gtcgcgggcc ccactgcgta gacgtctggc aagtccggca
      241 tcgtcacgcg tgtcggcacc gacgcggcca cagcgcgcgc aagctcgtgc gcggctcgcg
      301 ggctcgcgac catgatgacg tcgtacgagc cgtgccccac tgcgcgcgcg agcgcatcag
      361 gatctgccgc aggcgctggc ttcgtcacgg gcatcgccac agcctccaaa cccagcggga
      421 cgagcgcagc cgcatacgct tgcgcgtcca ccgagtcccg cgtgagcaca gccactgcca
      481 tgtcgcgacg ttatgccaac tggcacgccg tgtgctgttt cttctcgcat ggcggcgatc
      541 agttctccta ggatctaccg aaggcctgac acacccgtgg gatctggaac tgcgaaagat
      601 gtggagctgc agcgcgtggc caccgttgcg cgcgtgctcg acaactacat gatcgatccg
      661 ctcatcggtc tcgtgatccc gggcggcggc gacgtgatcg gctcgctgct aggcgtctac
      721 accgtcatga tcgcggtccg tcgcaaagtc tcgccggtca tcatcgcgcg catgatcatg
      781 aacctcggcc tcgacgccgt gatgggcttc atcccgttca tcggtgacct cgtcgacttc
      841 ggcttcaagg caaaccagaa gaacgtcgcg ctactctcgc agagcctcga gacgcgccac
      901 ggcaaggcga gcgcgaaaga ctggctcgtc gtcgccggcg cggccgccgc atacatcgcg
      961 atcatggcgt tcgtcgtctg gggaatcgtc aagatcttcc aggcgatctt ctaggcgcgc
     1021 taagtcactc ggggacgttc tcgaagcgga ctttcgcgcc tgcagcgact tgatgctgct
     1081 tcgaccagcc gccgttgact tcgaggacgt agagcgacgg cttctcgact tggcgcagcg
     1141 tctccgtctt gggctcggcg ttctcgacga taccgacgac ggtcttgtcg cgaccgatga
     1201 agagcatgtc gagcgggatg agcgtgttgc gcatccagaa cgaccacggc ttctctttgc
     1261 ccatcatgaa caacatgccg gcatcgaggc cgagatgctc gcggtacatc aggccgcgct
     1321 cgatcttcgt ctcggtcgcg acgacctcga cggtgacagc gacgtcgccg gtcgcggatt
     1381 ggatgtagac ggtcgggcgc gggcccgatg gctgcgtctg tgtggatgaa ccggacggct
     1441 tgggcggcat gtcgtcttgg cgcttcgcct cgttctggca agccacacac gcgagcacgg
     1501 cgatgacaac gaagcgcacg cgtggaagct accaatcagg gagcgcccga gccactacct
     1561 gcgttgtcgt tgatgaacgg cggcggctcc accgcaactt gcatggactg ttctggcggc
     1621 gctggattga ctttgatcga ctggcagaac gcgcgcatcg agtcgctgtg gcgctcggcg
     1681 tagcccatcg ccgccatgcc ttggacttcg acgggaaacg gcacgacgca cgccgagtac
     1741 gtgcccggcg cgacttcggt gaacttcgcc ggctcgccgt tgcgaatgat cacccactgc
     1801 gacgagctcg cgccagcggc ggccatttgc aggctgaggt ctgtcatcgt ccgcgccgcg
     1861 acgacgcccg tggcgagaaa cgcgctcgct acgccgatct ttgcgccgcc tttgccgacg
     1921 gcgttgactg cgagcgtgag tgcgcccgga tcggcggaga gatcgagcgt gacgtctttg
     1981 ctcggcggca cgacgatctc cttcgagtag aacttcatcc ccatcatcgg catgccgacg
     2041 gtggcggaga ctttgtacgt gtcaggcgcg aggcggtcgt agcgatacgc gccgtcgggg
     2101 ccggaggcga ccgcgtagat cgcgcccggc gtcgtcgtcg attggcagct gacgaacacg
     2161 ccttcggcgg gcttgccggc gacacgcagg atgcccgaca gcgagccgaa cttctgcagc
     2221 tcgagcacga gctcgccttg gccgggcatt gccgtcgaga cgcgcatcgc tttgctgcga
     2281 ccgatcgtgg gctgctcggc gacgaccgcg atgtcacctt cgttgagacc ggagatgacg
     2341 aacttgcccg atgcatcggt ggtgtccgtc ttcgtgccct gccccatcgg cccgaagttc
     2401 gcgctgttcg tcgagccgct gccgaagatc tggcggcctg cgtagacggt cgcgttggca
     2461 accggctggc cttcggcgac gacgacgccg gcgaggatgc ggcccttcgt cacgctgatc
     2521 gttccggcgt cggcggtctt gcccggatcg acggtgacct cgacggcctt cgtctgaaag
     2581 ctcggcccgc gcacggacag ctcgtacttc tgcggtggca atgcgtcgag gaggaactcg
     2641 ccgctcgtgc cggtgaacgc ctgctgcgtg aagccgacct ggatcgtgaa tgcgccgggc
     2701 gcactgccgt cggcgagctg caccttgccc ttcacggcac cttcgggctg gagcacgagc
     2761 ttcaggttgt tcgtgcccgt ctccgcgacc gtgccttcga tcgcatcgcg acggccgcgg
     2821 ctcgcattct gcgatcgcgt cgcgcgcacc atgtagctgc cgggcgcgag acccgtaagc
     2881 gtgaagtggc catcaccgtc ggtgagctcc tgcggaaagc cacgcaggcg ccattggctg
     2941 aagtcgcgcg cggcgccttg gtctttgaag tt
//
LOCUS       DATLBZ010000309        29164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1091829, whole genome shotgun sequence.
ACCESSION   DATLBZ010000309 DATLBZ010000000
VERSION     DATLBZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 29164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29164
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091829"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VMZ53_15795"
     CDS             <1..621
                     /locus_tag="VMZ53_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29969.1"
                     /translation="TIVFDLTIRDLQGYAWDFPTQVAGKPLICRGAYVLHHLGPDSPK
                     ERIAQYLAERGLDLASYRLKQFAERGFEPGEEVAKPRVLLVGEAAGIDIATGEGIGQA
                     IEYGYVAGPFLAKALERDNLLFSTWRSHLEGRHVGWQLRIRHWCYEMFFSHRRDMVER
                     MLPRMTALMKVGVQDFAGMPLSKLAIARGAAQFLSAWSREKMRAES"
     gene            618..1019
                     /gene="pth2"
                     /locus_tag="VMZ53_15800"
     CDS             618..1019
                     /gene="pth2"
                     /locus_tag="VMZ53_15800"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HUS29970.1"
                     /translation="MSERPTKQVIVMRKDLGMRKGKMIAQGAHAAMAVFTKDATISDN
                     EMRCELTDAMKAWLGPTEGPRFAKICVSVDSEAALDDVVARARAAGLPCALITDSGKT
                     EFHGIPTKTCCAVGPAWSDEIDAITGGLPLL"
     gene            complement(1060..2829)
                     /locus_tag="VMZ53_15805"
     CDS             complement(1060..2829)
                     /locus_tag="VMZ53_15805"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUS29971.1"
                     /translation="MRALVVMLAVGAACGRPSSPAHVTPSPGGITSSNILRADYAGSR
                     ACAECHKELFDRWAASPMRNMTRDAATATIRAPFDGATLRVGTETATMEMDGKVRVMR
                     VVDASGTHRFKITKVVGGRYREDFVGVEQGGDGLEHVLPATYVFATRSWRYKGYSVMV
                     KERPRMSWRGVWSRECIPCHNTLPYVTMLYDELHGPGLPGYQGKLSDRVLPPSRTWKA
                     AALDVPGLSRELDSEIAFLGGEKPQETELKPVLVTAATTMLKKLDGAHLVELGVGCEA
                     CHNGAKAHAHDSNITPAFEPRSSLVGITPPPGQSGTRTQWINRTCAKCHTVLFTHYDW
                     TWEGANRKDQHPGGSTTNSGEGRDFQLGGCSTQLACTGCHDPHVEDAPAKLAEMATPK
                     GNGVCTSCHKQFASEKAIEIHTHHASGSTGTSCVACHMAKKNMGLDYALVRYHRIGSP
                     TETRRVAGDRPLECALCHADKSVEQLVTTMEQWWHKKYDRDALKALYGDDLSVNAIRA
                     TLERGKPHEQAAAIGVLGDAKDKSAVAALVPMLSHEYPLVRFYAQRALQKITGDPVAI
                     DVGGAAADVQRDANAWLAKQKGP"
     gene            complement(2842..3474)
                     /gene="gstA"
                     /locus_tag="VMZ53_15810"
     CDS             complement(2842..3474)
                     /gene="gstA"
                     /locus_tag="VMZ53_15810"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF007831.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HUS29972.1"
                     /translation="MKLYYSPGACSLSPHIALREAERTFELERVDLRSKKTASGGNYL
                     DINPKGYVPALQLDGPGIEILTEGPAIVQYVADLVPDRGLAPANGTFARYHLQEWLSF
                     ISTEIHKSFGPLFAPDTPVPTQERSRSKLVERLAYTSKQIDGKPFVMGESFTVADCYL
                     FAVLRWCERFEIDLDDRLEDYFHRVFERPAVQAALQAEGLLETKRHRRSA"
     gene            3498..3719
                     /locus_tag="VMZ53_15815"
     CDS             3498..3719
                     /locus_tag="VMZ53_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29973.1"
                     /translation="MPVAAHALQPDSAMLATSRENTTRLAVQAVLASELVLLVLLAIA
                     LPMTKVAYVAAGLLFGQLLALLALPAHRP"
     gene            complement(3853..3978)
                     /locus_tag="VMZ53_15820"
     CDS             complement(3853..3978)
                     /locus_tag="VMZ53_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29974.1"
                     /translation="MRSNVAKRLHGSGFADPFCLSRRVLDGLRFGTDELAKELVE"
     gene            3988..4383
                     /locus_tag="VMZ53_15825"
     CDS             3988..4383
                     /locus_tag="VMZ53_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29975.1"
                     /translation="MRPRFNLVLDGHSLVTGRGGISATLSTLRQLVTDRIHDSERAYR
                     AALLDLRHGLDVVRVMREVARLEELFALIRWCDDWLAARRTLVSRVEAQLAWFAEQPS
                     AEDADSAMQPSEDPSGTDPDGPPAFLDSH"
     gene            4688..6874
                     /gene="otsB"
                     /locus_tag="VMZ53_15830"
     CDS             4688..6874
                     /gene="otsB"
                     /locus_tag="VMZ53_15830"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HUS29976.1"
                     /translation="MHAEWQHLASAASIVIAVDLDGTLIPFAPTPQEAIVDEQTAALL
                     DALVATPGVTLGVISGRPRELVTDLATRFPKVAFAAEHGVWRCASGEWEAALDPVPQL
                     DEIERALTGLAKRHPGALIERKTCSVCLHWRRVEEPYHSRISAAAEVIVDEWLETQPS
                     LERLPGVEMIEVRHRAAHKGSALAWLRSHGPAGSCVLAIGDDITDEDMFVSLRDNDVG
                     ILVASVPRRTQAGLRLPSIAAVHKFLYWLADARTQRPSNSPDDIVAARPPKPATPPQL
                     IVASNRLPAAPTGDRKQEVGGLVSAILPALAKTHGVWLGWSGAERDPGLKLRVEPNDS
                     FTRAQFDYPLTWRQRYYAGFCNRCLWPLLHGFMRVHYADDEWACYQDANRAYARMLME
                     LGGTTADTWVQDFHLMLVGREMRKLGHRGRIGFFLHVPFPALDVLETMPWASELVSAM
                     LDFDRIGLQTQRWADNFIATARGLLGHEAEVRAKERVRVVPVGIDPDRFAEAAAAAAA
                     KVANANPNESVTLGLEAMLGGRKLILGVDRLDYSKGIPERLEAFARLLEKYPEWRNQV
                     SFVQVSVPTRSDVPEYGELRGRVESLVGRINGAYGEADWVPVRYLYRSYDQETLARLY
                     RSAAVGLVTPLRDGMNLVAKEFVAAQDEADPGVLLLSRFAGAAEHLTLALLTNPYHAD
                     GLASDLDTALRMPREERITRNSALRTIVWRDTAAAWAERFLEDLRA"
     gene            complement(6871..8466)
                     /locus_tag="VMZ53_15835"
     CDS             complement(6871..8466)
                     /locus_tag="VMZ53_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HUS29977.1"
                     /translation="MTNVNWTELVIMGVLFAVVAIMGFVAAKWRAPKTMEHLDEWGLG
                     GRSFGGWVTWFLIGGDLYTAYTFVAVPALMFAMGAAGFFAMPYTVITYPLVFLPLIRL
                     WSVSHKHGFVTPADFVRARFGSPTLALLVAVTGIVATMPYIALQLVGIEAVLETIGLT
                     GDSFVARHLPLIIAFAILAAYTYQSGLRAPALIAFVKDTLIYIVIIAAVLWIPHQLGG
                     WDKIFDAAQHKFETTKKGSFLLPEQSQLQYITLAFGSALALFLYPHSLTGILASRSRD
                     VIKRNMAALPAYSLVLGLIALLGFMAIAAADSHGVKPVIVDGKPHVSTIVPMLFHKMF
                     PPWFAGIAFATIAIGALVPAAIMSIAAANLATRNVYLEYINKQATPAQQAQVSKVVSL
                     VVKFGALGFILLVDPQYAIDLQLIGGVIILQTLPAVAIGLFTRWFHRVGLIAGWIAGM
                     GAGLAMLYTIPNPAAGRAHFGGSAFPLAKLGFDTKMTIYAGFVAVFVNLLVTIIVTLI
                     ARAMKIQDGPDATSKADYFSERA"
     gene            complement(8463..8690)
                     /locus_tag="VMZ53_15840"
     CDS             complement(8463..8690)
                     /locus_tag="VMZ53_15840"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HUS29978.1"
                     /translation="MSEKPETRDDEHSDRNPWNWLLLVPIVVPLLTFLYNDAEPRLFG
                     FPRYYWLQIAFVGLGVLCTTIVYRMTRHRKP"
     gene            complement(8687..9160)
                     /locus_tag="VMZ53_15845"
     CDS             complement(8687..9160)
                     /locus_tag="VMZ53_15845"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUS29979.1"
                     /translation="MALTLAEAAFAKKRDLVRKMLADSVPVDSNEAPHHRTALHCAVM
                     VNDLEMVNMLLAGGANADAADFVGDRPIHFAPQHKVRLPVLDRLLEHAPHLVNRAGAI
                     GRTPLHVAVDLKCAEAIERLLAAGADPSIKDATGKIPIERAETAKIRKLLAKMRP"
     gene            complement(9170..9481)
                     /locus_tag="VMZ53_15850"
     CDS             complement(9170..9481)
                     /locus_tag="VMZ53_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29980.1"
                     /translation="MRNARAFVLGVAAVIASGAGCAPRQPPMVTATDAQRGNVELAEL
                     QQGRKLLIGKCTNCHRAPMPNDHTAADWPQKLDEMASRASLDFSQQKLIEKYLVTMST
                     R"
     gene            complement(9484..10248)
                     /locus_tag="VMZ53_15855"
     CDS             complement(9484..10248)
                     /locus_tag="VMZ53_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29981.1"
                     /translation="MSFSARLLVVGGALAATTFLDSTAHADRRMFTSTYEYKTVPEGH
                     TALELWHTESRQTWDNTTPQALEHILEIEHGLTDHWDLAFYTVFDQTAGGTVAGVATE
                     SEPFHLAEMKLETRYRFADRGELPVDILAYGELVKVFGESIYEVEAKGIFARDFDKVT
                     VALNVIAEIMLGKNAPETEPEFGWALGASYEVHPKVNVGVETYGEIEEEEVGASIGPA
                     LAIAPSGNFWFTFTAAFGLTDEAPALSGRLIVGVEL"
     gene            complement(10322..12064)
                     /locus_tag="VMZ53_15860"
     CDS             complement(10322..12064)
                     /locus_tag="VMZ53_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUS29982.1"
                     /translation="MPQHGGDLIADVLARHGVTHLFTLCGGHISPILTGAQARGIKVV
                     DVRDEGNAVFAADAVARMTGTIGVAAVTAGPGVTNTITAIKNAQMAQSPILLFGGATA
                     TLLKGRGALQDIDQIELMKPLVKWATTVKTVPSLVHTVERAIQIATEGVPGPVFVEVP
                     VDLLYSEEIVRGWYEKEAGLGKGKGIGAKALELYIRGHLYRQFRAPAMPEIDLGPMKL
                     PKLRGDGLIDRAAEALRTAKKPVIVVGSQTLVGVKDPGRLARAIGQLGAPVFLAGVAR
                     GLLGRTHELQFRHNRGAALKEADCVIVCGFPFDFRLGYGRGINKQGTLIAANLSTHEL
                     RKNRRPDIAIEMHAGDFLVALAERAGGGAGAWTDWVSTLRDREASRDSEITAKSAPKG
                     ELVDPIHFFTRLDEHMADDAVLVVDGGDFVATASYIVRPRAPLSWLDPGVFGTLGVGG
                     GFALGASLVRPGREVWLVWGDGSSAYTLAEFDTYVRHGVAPIALIGNDASWMQIAREQ
                     VEILGTSLGTDLRRSDYHKVAEGYGGVGLLLTDPARVDSTLAEAKAIAKGGRPVCINI
                     HLRKTDFRKGSISM"
     gene            12110..12994
                     /locus_tag="VMZ53_15865"
     CDS             12110..12994
                     /locus_tag="VMZ53_15865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HUS29983.1"
                     /translation="MSNLVLYAIPAFLALIILEAFWARRHPEDIVGYERRDTVASISM
                     GALNVVVSAVAKLASIPFFAWLYTHRIADLGQPAAWWSWLLLLVAEDFCYYWFHRSHH
                     EVRLLWAAHVNHHSSQHYNLSTALRQALLTPVTGPIFWAPLALLGFPPVMILTAQAWS
                     LLYQFWLHTEAIKTLGPLEWIMNTPSHHRVHHGKNVVYLDRNHAGIFIIWDRLFGTFQ
                     KEEERVIYGLTKDIHTFNVFQIGFHELVAITRDVARSRRLGAKLGYIFAPPGWSHDGS
                     TKTAGQLHKELRAPRSSL"
     gene            complement(13277..13906)
                     /locus_tag="VMZ53_15870"
     CDS             complement(13277..13906)
                     /locus_tag="VMZ53_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29984.1"
                     /translation="MSAGFGGAPFGDAFTAAAPHSAVQSSANVMLFKDPIGDTHRQLD
                     EARAFKPSILIAVDLLFWDVYGGFDTAWHDKALADSLAELEKLRAAGVWIVLGDIPLI
                     TTASEMMLPKDAIPTQARLDAANKAIAAWAKKDHVVLVPLVEWTAPLRTGAKVKLPTG
                     ETVEAAELMAMDGLHANPLGTWYLLDKLDHYLERKVAATPDALVFKRPS"
     gene            14932..15726
                     /locus_tag="VMZ53_15875"
     CDS             14932..15726
                     /locus_tag="VMZ53_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29985.1"
                     /translation="MDPGLETALFTPQLCTNTNAVPGDPDCPINIVPLDGLVQGWAGA
                     RIKFVAQPLSSALYVTNLSLEGGADGVYIEHPLFVSWPLTEGAQPIPDQLDRFFNVKM
                     NLMTGSMATPIQGGTAAFINFMPNNKLTINFKVVDKFRPEDGGMGGMTMASGCKQLAS
                     FKTNARGPLQTNCASCHAAAANTNARGAMDITGIAAMDDATLQTVCNQARTRVNFTDT
                     NQSGFFIAPNPAQATNHPFKFGNVQANFDNFKTAVDVWVQAEKTSQ"
     gene            15745..16914
                     /locus_tag="VMZ53_15880"
     CDS             15745..16914
                     /locus_tag="VMZ53_15880"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HUS29986.1"
                     /translation="MKRISALLLAAASFAVPACSSEQGDDSVGSGSGSGSGSETGDEW
                     DQALSQREYDYNAALRIAALRLTGALPTMAEIQEIASAPDNATKKGLYEARIQQYMSR
                     PEFGKQMMYFWRDTFKMGETAMLDTAPAFATGLSINNGSYMDMFTASSGNCPTFDMTA
                     GTVASAECMNGGPKAGLLTNPGIQSHYFGNLGFRRSRFFQETFVCTKFPAEVGTAPVD
                     VGGAAPYTGVWPFQSIASPTNGGGRINFQDTSAVICANCHSTLNHLTPLFAYYDDQGA
                     YQTAISVPTPLDGAPLAKMTDYLPAGETTAWRLNVPAADLPALGQAMAADADVAECGV
                     ARMWNWALGKTDIVDTLQEVPSETIAAQVDAFKANGFKMKDMIFAVYTSDDFVKF"
     gene            16926..17606
                     /locus_tag="VMZ53_15885"
     CDS             16926..17606
                     /locus_tag="VMZ53_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29987.1"
                     /translation="MKTIGYTLAILASSVLASACTDDTTTTNEPPTGSTSGNEDTTFD
                     HDNGDYISPWELLDRLQKEGPPRYTSKVHSCSKVRYKTLGTVLTNVGMNPANATALSA
                     GNLYTTGFNALGGPNYANRIRENISITTSGASREFDIFAAGAQETIDAFANQTGIAGT
                     GGRCAGITLFDASNNCRPDGITCLTGVPATALHLDFCNKTVSMASDVTVGKRLAVADM
                     LAAAYTCE"
     gene            17618..19006
                     /locus_tag="VMZ53_15890"
     CDS             17618..19006
                     /locus_tag="VMZ53_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29988.1"
                     /translation="MAKSPISKRDEEREITRRALIKWSVAAGAALGVSRAKVFEILER
                     TAGRDVAFAAAENPTTRSVHIIAGNGGNAWFQLLWPHNDIAAANNTTFAWHRPGESRL
                     VAGTDKPLTVGPDTPFADMPAARQMTCFQMGNNETHTGAPTSVTSLNGNNIISVASAL
                     QSAAPSVIPMIAIGGVDVGTAPGAARPAAVGNADGVVGLFNSAASRAGGLLSKAQDAT
                     LYKAHYDAFAQLNRAQNRSTQKTAYSTASNAAQFLGTNLAAKLQIVQADLDRYGIDGT
                     TRANVRDIGRALIVSVKAFKMGLTNSVSLPAMRDDPHGAFADGSAMSVPPMLKKVFDG
                     FMNDLKITVDDNTGKVLADDTVITITGDTTKNPLQRGGWPDGTPNNSNVMWVYSAGHL
                     MSGWFGGTLRNGTAQGFDANGATAAYNGANTARMALASVAYAIAKRDDRAITAFANGI
                     KVGGVFGRLKDL"
     gene            19099..19722
                     /locus_tag="VMZ53_15895"
     CDS             19099..19722
                     /locus_tag="VMZ53_15895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS29989.1"
                     /translation="MRVWLVIVLLFAVACEKKPAGSGTTGSASSPLNDEAAREQARFD
                     AERRPDKVVEALAIKPGSVVADVGAGSGLLTVHLARAVAPNGKVVATDVDGGVLGFLN
                     QRLKSQNLANVVETRVVAADTPGLEPATYDAILLAEVDHYFTDPAAWLREAGKALKPE
                     GRIVISNRIYHRAQTMASAKKAGLVLLTETTPVPTHFIAVFTTEAKK"
     gene            19719..19865
                     /locus_tag="VMZ53_15900"
     CDS             19719..19865
                     /locus_tag="VMZ53_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29990.1"
                     /translation="MKLSPLLLLALAACPGDDGDGGNAITLWLAPDGRETEVKLVESE
                     PTPY"
     gene            19875..20243
                     /locus_tag="VMZ53_15905"
     CDS             19875..20243
                     /locus_tag="VMZ53_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29991.1"
                     /translation="MGAGGSQEAEYSLKGSVPAGTYHCVLDSIIIRPVDVTFDLIWRR
                     GTTDMPLATWMQHFEPLPGSFDAQAYEIDQAAPAIDFAAGDQLIFRYSGANTTSSEAY
                     IPNGDGSLSHGRIPNITLPK"
     gene            20284..22176
                     /locus_tag="VMZ53_15910"
     CDS             20284..22176
                     /locus_tag="VMZ53_15910"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HUS29992.1"
                     /translation="MKVLGLVVAMVVAAAPAFAGEQRAWVKEVGNLDTWKHYSKTVGT
                     DEFGKAVIDVKTNEIYFFDVNLFNIHADFVLGVLLKQPWTATNVREYNKNYERDKPRF
                     ILCYLTHHLKVDKWTFSFWEGDKIGPDDVMKVSKRFEQTFFMKKLPFRPDSPLQMKVA
                     ADVKKKGLEIITNDDIYKAADFQAFNKGKTVGRLRVVPVGTAFESLTFDRHDIVLLQE
                     SYPDITPVAGIIATQFSTPLSHVNLRAGAWGIPNAGDKKAREKFGKLDGKVVYFEVTD
                     TKDVMREATKEEAKQLEQSIYSAKHVDLPEADITTKRMAMLTRMRAKDVLRYGTKASN
                     LGEIVTANLDGVNVPPGFGVPFAFYVQHLQKNGLDKKIEAMLADKKFAVDPAWRKTAL
                     EEIRKAITDAPIDADTLDMIYKRVRIKLGGKGVFVRSSSTSEDLPGFNGAGLYDTVGN
                     VRGKKALGDALKVVWASLWNLRAVDERSAFGIDHRQAYMGVLVQVGVSATAAGVLVTK
                     NLWDPGDDHSFTINSKWGLGERVVQGVKMPEQIVFDPTNDGTKIISRADDPVMLVFDD
                     KGGVKELPVPAGAGVILTEERAKKLSQMVQKFIPVFTHGQPLDVEWVLEGEEVWIVQA
                     RPFVGS"
     gene            22176..22661
                     /locus_tag="VMZ53_15915"
     CDS             22176..22661
                     /locus_tag="VMZ53_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29993.1"
                     /translation="MRRALVLIALAACELQPAPKKQPVPVEVVTPPPQPVQAPVVVDA
                     GAAASDAEAPPPIIQASADCINVAAHVVQVFISTARDQAQAAVYEQERMKMTRATAEA
                     CEKQQWSTAAQKCYLASKNPAEIKACENKFPPPPPEREGNGSAVVKPTNEPGGPAVPP
                     R"
     gene            complement(22658..23119)
                     /locus_tag="VMZ53_15920"
     CDS             complement(22658..23119)
                     /locus_tag="VMZ53_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29994.1"
                     /translation="MTHDLLQTLGLYAGTLVVCFLAGIIPLINAEVYLVGISIWVVKR
                     PEQLPAIIILAALGQMIAKVILYYAGMGMFELPTGKWKARIDRARGMLTRWEKQPYLV
                     YAASASLGLPPLYLTTFAAGALKINFTLFCLIGFTGRVLRFAVLVSIPWIF"
     gene            complement(23201..24019)
                     /locus_tag="VMZ53_15925"
     CDS             complement(23201..24019)
                     /locus_tag="VMZ53_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS29995.1"
                     /translation="MNLAIAILTGTLVGLHMGTWGMYKDAPHEGFTWPKYFRSAIVAS
                     IIGGVTHHFVQWDLAHDAGKRFVYFGLIYCLERAATEFWKYFIREEDQSKYFIPMQFG
                     LFGKPMKDAKKRISIGILVAIACIGVFYGIRALEKSWGTTWPMWLVVITVGSFFGWIS
                     AFGGAWKDAPIEGFETFKFFRSPGVAASWAFVVCQFTSSWTIIAIAAEGFTVMAIETY
                     KTFFFPNKPRGKFAGKPILHPEYLELRKRFLPLYFGIWALVIVHFVWGMVGRTS"
     gene            complement(24016..24948)
                     /locus_tag="VMZ53_15930"
     CDS             complement(24016..24948)
                     /locus_tag="VMZ53_15930"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUS29996.1"
                     /translation="MIRLAFLGCGKIAKAHAKRLRAHKADVEVGFSSRDPQRAQAFAR
                     ELDGAAHGTYEEAIASPDVDAVAIVTPPDSHLPLALAALAAGKHVVLEKPPVPRASDF
                     TQIAEAAAAANKQVFVAENYQYKPLLRRIRQVLADGLIGEPLFAHVNAIKLQKSDAWR
                     TDYGALYEGGIHWIDFMSSLGTVRGARGLHPSNGAKPERSMLVTFDYAEGFVGSLAYS
                     WEVPSTAKGLRLSKIYGRAGSVTFETNGLWVFLHGKKTRFYVPGLRDLAGYNAMWADF
                     VQAWKSGTEPAMTLARARRDLELVEEAYATAGVS"
     gene            complement(24945..26483)
                     /locus_tag="VMZ53_15935"
     CDS             complement(24945..26483)
                     /locus_tag="VMZ53_15935"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HUS29997.1"
                     /translation="MKWDAVIVGSGFGGAMVAHRLVEAGARVLMIERGDWVSRGEAAW
                     RPEASFELTPSYARDIPYRCERGGHGQYIGAVTCVGGPSVYYGCVSFRFREQDFTGDR
                     DVVADSGAAFPYRYDEIEAEYAEAERLLGVSGSDAGDPTAPPRSTPFPQPPAELAPLS
                     MRVAETAVHMGLSPFHLPLAINHAVNDTRKACVACRTCDTFACAIEAKNDVATTMIAP
                     LVARGMTLWTNTVALRLETNDTTIKRLHVLRRDTGERVELQAYQYVLAAGALGSPHLV
                     LSSRLDELSPARGAVGRYLMRHDNAMVFGVFPERPDPHRRFHKQLAIHDWYFGDPSDH
                     EGGHLTKLGGVQQVMTPPKELVRAHLPIGTRSLLGTFTEQLTGLLAIAEDQPRLDNGV
                     ALDWDVRDQYDLPQLVIGTSYSARDGLAVDVLARRAREILSKMGAVVSYTHRVKTFSH
                     AVGTLRMGVDPATSPLDEHCRFRGVDNLWVTDGSALPLSAGVNPSLTIAANALRAGTA
                     MTHS"
     gene            complement(26503..>29164)
                     /locus_tag="VMZ53_15940"
     CDS             complement(26503..>29164)
                     /locus_tag="VMZ53_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUS29998.1"
                     /translation="GGVRGGAQILTLVVQLFVARGLLARIGTGRALLLAPFVALASGL
                     GLVVAPILAVAIATQASAKVLDSAIETPAEKLAQTLLPTVVRGRVAGFLEGTAKRAGA
                     VVGGLIAAALASSPTVFYVITAVAAALWLLASLRISRELPALAIEHVAETRSRTDALE
                     EGAVIDDRAVQALIKELDGPRPERAAEVLVRLHDQGRTDAVTPLVKAATETGMPSVWA
                     ALVATLDAPAEVHGKTLLEAAQAAKPKLRELAIRALGLAGGVPPDAFDQWRDPDRVRS
                     SVTEAAGASSGELDRVSDPIPAVTADARPGSRARRDSVTGGAATVAMTAEVARLRVAG
                     DWDALIGELADASRESDPIGRVAIDELVSEIRRQDSAGQHERVLEAARHLTRALRRGR
                     GDGASRTAAFGTLARVVEELRAQRSAELSLMRADLLELVRDRVEQGASKAAPDTALVS
                     LIHKPVSAGHADEAVEVAAALRLYGELLEGADTVDGDDLRRITRALGEPDDEVRLAAE
                     AALVGLGPAAAGELLATVAWGRRRARDRAAALLADLPVTPATIDRLVDAELDALEQTA
                     TAITVFTQAGDELVARRLDERLREIAHTVLLLVAARRRSTAISRAAVAWQHARAGQER
                     ARALAVIEAAMPRALVTRLVEAVDDVTPSDRKAAFVRAGGELPSRDAVVKAELAGRDR
                     LSRALVLHVLGAVDRSAHRETIAKAAQAEALAASPTELLRRLTKMTEPTADTPVDEGA
                     SDMPSRVETLIALGRVPLLAGLSTRQLADVAERARWMNAREGSVVVSGGDPIDALIVV
                     EDGELQIGDRRIAKGEVVDELACVAPAAAAADLTVIRSARLIRLERVDFEELVDDVPG
                     LASSVCRALGERARRAEDASYRSPLASRAG"
BASE COUNT         5150 a         9710 c         9416 g         4888 t
ORIGIN      
        1 acgatcgtct tcgatctcac gattcgcgat ctgcaaggct acgcgtggga cttcccgacg
       61 caggtcgccg gcaagccgct gatctgccgc ggcgcgtacg tgctgcacca cctcgggccc
      121 gactcgccga aggaacgcat cgcgcagtac ctcgccgagc gcggcctcga tctcgcgagc
      181 taccggctga agcaattcgc cgagcgcggc ttcgagccag gcgaagaggt cgcgaaaccg
      241 cgcgtgctcc tcgtcggcga agcagcgggc atcgacatcg caaccggtga aggcatcggc
      301 caagcgatcg agtacggcta cgtcgcaggc ccattcctcg cgaaggcgct cgagcgagac
      361 aacctgctgt tctcgacgtg gcgctcgcat ctcgaaggtc gtcacgtcgg ctggcagctc
      421 cgcatccgcc actggtgcta cgagatgttc ttcagccacc gccgcgacat ggtcgagcgc
      481 atgctgccga ggatgaccgc gctcatgaag gtcggcgtgc aagacttcgc gggcatgccg
      541 ctctcgaagc tcgcgatcgc gcgcggcgcc gcgcaattcc tctctgcatg gagtcgcgag
      601 aagatgcgag ccgagtcatg agcgagcgac cgacgaagca agtcatcgtc atgcgcaagg
      661 atctcggcat gcgcaaaggc aagatgatcg cgcaaggcgc gcacgcggcg atggcggtct
      721 tcacgaagga cgcgacgatc agcgataacg agatgcgctg cgagctgaca gacgcgatga
      781 aagcgtggct cggcccgacg gagggcccgc ggttcgcaaa gatctgcgtg tccgtcgaca
      841 gcgaggccgc gctcgacgac gtggtcgctc gtgcccgcgc cgccggcctg ccctgcgccc
      901 tcatcaccga ctcgggcaag acggagtttc acggcatacc gacgaagacc tgctgcgcag
      961 tcggccccgc gtggtccgac gagatcgacg cgatcaccgg cgggctaccg ctgctctagc
     1021 aaagcgcgtc gcgccattca cgcgctacac gacgactcat cacggacctt tctgcttggc
     1081 gagccacgca ttcgcgtcgc gctgaacatc ggcggcagct ccgccgacgt cgatcgcgac
     1141 tggatcgccg gtgatcttct gcagcgcgcg ctgcgcgtag aaccgaacga gcgggtactc
     1201 gtgcgagagc atcggcacga gcgcggcgac cgcggacttg tccttcgcat cgccgagcac
     1261 gccgatcgcg gctgcttgct cgtgcggctt gcctcgctcg agcgtcgcgc ggatcgcgtt
     1321 gaccgagagg tcgtcgccgt agagcgcctt cagcgcgtcg cggtcgtact tcttgtgcca
     1381 ccactgctcc atcgtggtga cgagctgctc gacagactta tcggcgtggc acagcgcgca
     1441 ctcgagcgga cgatcgcctg cgacgcggcg agtctccgtc ggcgatccga tgcggtgata
     1501 ccgcacgaga gcgtagtcga gccccatgtt cttcttcgcc atatgacacg ccacgcacga
     1561 cgtgccagtg cttccgctcg cgtggtgcgt gtggatctcg atcgccttct cgctcgcgaa
     1621 ctgtttgtgg cagctcgtgc acacgccatt gcctttcggc gtcgccattt ccgcgagctt
     1681 cgccggtgcg tcctcgacgt gcggatcgtg gcagccggtg cacgcgagct gcgtcgagca
     1741 tccgccgagc tggaagtcgc gaccttcgcc ggagttcgtc gtgctaccgc cggggtgctg
     1801 gtctttgcgg ttcgcgcctt cccacgtcca gtcgtagtgc gtgaacagca ccgtgtggca
     1861 cttcgcgcac gtgcgattga tccattgtgt ccgcgttccg gactgtcctg gcggcggcgt
     1921 gatgccgacg agcgacgagc gcggctcgaa cgccggcgtg atgttgctgt cgtgcgcgtg
     1981 cgccttcgcg ccgttgtgac aggcctcgca gccgacgccg agctcgacga ggtgcgcacc
     2041 atcgagcttc ttcagcatcg tcgtcgcggc ggtgacgagc acgggcttca gctccgtctc
     2101 ctgtggcttc tctccgccga gaaatgcgat ctcgctgtcg agctcgcgcg ataggcccgg
     2161 gacatcgagc gcagcagcct tccacgtgcg cgacggaggc agcacgcgat cgctcagctt
     2221 gccctgatag ccggggaggc ccggcccgtg cagctcgtcg tacagcatcg tcacgtacgg
     2281 cagcgtgttg tggcagggga tgcactcgcg cgaccacacg ccgcgccacg acatgcgcgg
     2341 gcgctccttc accatcaccg agtagccctt gtagcgccac gacctcgtcg cgaacacgta
     2401 cgtagcgggc agcacgtgct cgaggccgtc cccgccctgc tccacaccga cgaagtcttc
     2461 ccgataccgc ccgccgacga ccttcgtgat cttgaagcgg tgcgtcccgc tcgcgtcgac
     2521 gacgcgcatg acgcgcacct tgccgtccat ctccatcgta gccgtctcgg tgcctacgcg
     2581 cagtgtcgcg ccgtcgaacg gagcacgaat cgtcgcggtc gctgcatcgc gcgtcatgtt
     2641 gcgcatcggc gacgcggccc agcgatcgaa cagctccttg tggcactccg cgcaggcgcg
     2701 cgagccggcg tagtcagcgc gcaggatgtt gctgctcgtg atgccacccg gtgatggcgt
     2761 cacgtgcgca ggcgacgacg gccgaccgca cgcggcgccc accgcaagca tcacgacaag
     2821 cgcacgcacc cagcgtgctt atcacgccga ccgacggtgc cgcttggttt cgagcaatcc
     2881 ctccgcttgg agagctgcct ggacggcagg acgctcgaac acgcgatgga aatagtcctc
     2941 gagccggtcg tcgagatcga tctcgaatcg ctcgcaccag cgcagcaccg caaagaggta
     3001 gcagtcggcg acggtgaacg actcgcccat cacgaacggc ttgccgtcga tctgcttcga
     3061 cgtgtacgcg agccgctcga cgagcttgct gcgagaccgc tcttgcgtcg gcaccggcgt
     3121 atccggcgcg aacagcggac cgaacgactt gtggatctcc gtcgagatga acgagagcca
     3181 ctcctgcaag tgataacgcg cgaacgtgcc gttggccggt gcgagcccgc ggtccggaac
     3241 gaggtcggcg acgtactgca cgatcgcggg cccctcggtg aggatctcga tgccgggtcc
     3301 atcgagctgg agcgccggca cgtagccctt cgggttgatg tcgagatagt tgccgccgct
     3361 cgcagtcttc ttcgatcgca gatcgacacg ctcgagctcg aatgtccgct cggcctcgcg
     3421 cagcgcgatg tgcggcgaca gcgagcacgc gccgggggag tagtacagct tcatatccct
     3481 gagagagagc atcggacatg ccagtggcgg cacatgcgtt gcagcccgac agcgcgatgc
     3541 tcgctacctc ccgagagaac acgactcgcc ttgcggttca ggcagttctc gcgagcgagc
     3601 tcgtgctgct cgtcttgctc gccatcgcgc tgccgatgac gaaggttgcc tatgtggccg
     3661 caggcctact ctttggccag ctattggcgc tcctggcact acccgcacac cgcccgtagc
     3721 cgaaacgcac aacgagcgca actgacacgc agcgcgcggg cggcgcttcg ggtttgtgcc
     3781 catttgcgcg gcatgctcgc tgcaccggtc gcgcgtatga gcacccgcga cgcggttagc
     3841 gaccgccggc gactactcga caagctcctt cgcgagctcg tccgttccga agcgcaggcc
     3901 gtcgagcacg cgccgagaga ggcaaaacgg atcggcgaaa ccgctcccgt gcaggcgctt
     3961 cgcgacgttg ctgcgcatgc cttgtcgatg cggccccgat tcaatctcgt cctcgatggg
     4021 cactcgctcg tcacgggccg cggcgggatc agcgcgacgc tctccacgct tcgtcagctc
     4081 gtcactgatc gcattcacga ctccgagcgc gcgtatcgcg ctgctctcct cgatctgcgc
     4141 catggcctcg acgtcgtgcg cgtcatgcga gaagttgctc gtctcgaaga gctcttcgca
     4201 ttgattcgct ggtgcgatga ttggctggcc gcacgccgca cactcgtctc gcgcgtcgag
     4261 gcacagctgg cgtggttcgc agaacagcca tccgctgagg acgccgattc agcgatgcaa
     4321 ccgagtgaag atccaagcgg tacagacccc gacggaccac cggctttttt ggattcgcac
     4381 tagcccgctc gtggcacgtc gatcgcagta ccaatagtca ggcacaccaa gaacgaagag
     4441 acgaacagcg aaagcaatcg gatcttcgaa tcacaagatt caatcaggtg atgtggtgat
     4501 tcacgatggc taggtcacgt tgaatccctg cgcttgggag tgctcttttg gctcgagtat
     4561 gcgaaggcag aaagagccac catttttggc tccgtcggtg cgaatcgacg tcgagccatt
     4621 tcttttttcg gcgtgagcta cgcgcaaggt tggacaacca actaacgcgc cgggttaagg
     4681 tgccgccatg cacgccgagt ggcagcacct ggcgtcggcg gcatcgatcg tgattgcggt
     4741 cgatcttgac ggcactttga tcccgttcgc gccgacaccg caagaagcga tcgtcgacga
     4801 gcagaccgcc gcgttgctcg acgcgctcgt cgcaacgccg ggtgtgacgc tcggcgtgat
     4861 ctccggccgc ccgcgcgagc tggtcaccga cctcgcgaca cggtttccaa aagtcgcatt
     4921 cgccgcggag cacggcgtgt ggcgctgcgc gtccggcgag tgggaagccg ccctcgatcc
     4981 ggtgccgcag ctcgacgaga tcgagcgagc gttgaccggt ctcgcgaaac gtcatccagg
     5041 cgcgctgatc gagcgcaaga cctgctcggt ctgcttgcac tggcgccgcg tcgaagagcc
     5101 gtaccactca cgcatctcgg cggcggccga ggtcatcgtc gacgagtggc tcgagacgca
     5161 gccatcgctc gagcggttgc cgggcgtcga gatgatcgag gtcaggcatc gcgctgcgca
     5221 caagggctcg gcgctggcgt ggttgcgcag tcatgggccc gcgggctcgt gcgtgcttgc
     5281 catcggtgac gacatcaccg acgaggacat gttcgtctcg ctgcgcgaca acgacgtggg
     5341 catcctcgtc gcgtcggttc cgcgtcgcac gcaagcgggg ctgcgcctgc cgtcgatcgc
     5401 ggcggtccac aagtttctct attggcttgc cgacgcgcgc acgcagcggc cgagcaattc
     5461 ccccgacgac atcgtggccg cgcgcccgcc aaaaccggcg acgcctccgc aactgatcgt
     5521 cgcgagcaat cgcctgcccg ccgcgccgac gggcgatcgc aagcaagaag tcggcggcct
     5581 cgtgtcggcg atcctgcccg cactcgccaa gacgcacggc gtgtggctcg gttggtcggg
     5641 cgccgaacgc gatccgggcc tcaagctgcg cgtcgagccg aacgattcgt tcacgcgtgc
     5701 gcagttcgac tatccgctca cgtggcggca gcgctactac gcgggcttct gcaatcgctg
     5761 cctctggccg ctgctgcacg gcttcatgcg cgtgcattac gccgacgacg agtgggcttg
     5821 ctaccaagac gcgaatcgcg cgtacgcccg catgctgatg gagctcggcg gcacgacagc
     5881 cgatacgtgg gtgcaggatt tccacctgat gctcgtcggt cgcgagatgc gcaagctcgg
     5941 ccaccgcggc cgcatcggct tctttctcca cgtgccgttt cccgcgctcg atgtgctcga
     6001 gacgatgccg tgggcgtccg agctcgtgtc ggcaatgctc gacttcgatc gcatcggctt
     6061 acagacgcag cgctgggcgg acaacttcat cgcgaccgca cgcggcctgc tcggtcacga
     6121 ggccgaggtg cgagccaaag agcgagtgcg cgtggtgccg gttggcatcg atcccgatcg
     6181 cttcgccgag gcagcagccg ccgcagccgc gaaagtggcg aacgccaatc ccaacgagtc
     6241 cgtcacgctc gggctcgaag cgatgctcgg cggtcgcaag ctcatcctcg gcgtcgaccg
     6301 cctcgattac agcaagggca tcccggagcg cctcgaagca tttgctcgct tgctcgagaa
     6361 gtatcccgag tggcgcaatc aagtgtcgtt cgtgcaggtg tcggtgccga cgcgatcgga
     6421 tgtgcccgag tatggcgagc tgcgcgggcg cgtcgagtca ctcgtcggtc gcatcaacgg
     6481 tgcgtacggc gaagcggatt gggtaccggt tcgctacctc tatcgcagct acgaccaaga
     6541 gacgctcgcg cgcctgtatc gctcggcggc cgtcggtctc gtcacaccgc tgcgcgatgg
     6601 catgaacctc gtcgcaaagg agttcgtcgc cgcgcaggac gaggcagacc ccggcgtgtt
     6661 gctgctctcg aggttcgccg gcgctgccga gcacctgacg ctcgcgctgc tcacgaaccc
     6721 gtatcacgcc gacggtctcg cgagcgatct cgacaccgcg ctgcgcatgc cgcgcgaaga
     6781 gcggatcacg cgcaattcgg cgctacgtac gatcgtgtgg cgcgacaccg cggccgcgtg
     6841 ggccgagcgc ttcctcgaag atctccgcgc ctaagcgcgt tcgctgaagt agtccgcttt
     6901 cgaggtcgcg tcggggccgt cttggatctt catcgcgcgc gcgatgagcg tgacaatgat
     6961 cgtgacgagc aggttcacga acaccgctac gaacccggcg tagatcgtca tcttcgtatc
     7021 gaagccgagc ttcgccagcg ggaacgccga accgccgaag tgtgcgcggc ccgcagcggg
     7081 attgggaatc gtatagagca tcgcgaggcc tgctcccatg cccgcgatcc agccggcgat
     7141 gagcccgacg cgatgaaacc accgcgtgaa gagaccgatc gcaaccgcgg ggagcgtctg
     7201 caggatgatc acgccgccga tgagctgcaa atcgatcgcg tactgcggat cgacgagcaa
     7261 gatgaaaccg agcgcgccga acttcacgac gagcgagacg accttgctga cctgcgcctg
     7321 ctgcgcgggt gtcgcctgct tgttgatgta ctcgaggtag acgttgcgcg tcgcgaggtt
     7381 cgcggccgca atcgacatga tcgctgcagg cacgagcgca ccgatcgcga tcgtggcgaa
     7441 cgcgatgccc gcaaaccacg gcgggaacat cttgtgaaat agcatcggca cgatcgtcga
     7501 gacatgcggc ttgccgtcga cgatgaccgg cttcacgcca tgcgaatcgg ccgccgcgat
     7561 cgccatgaaa ccgagcagcg cgatgagccc gagcacgagc gaatacgcgg gcagcgcggc
     7621 catgttgcgc ttgatgacgt cgcggctgcg actcgcgaga atgccggtga gcgagtgcgg
     7681 atagaggaac agcgcgaggg ccgagccgaa cgcgagcgtg atgtattgca gctgcgattg
     7741 ctcgggtagc aggaagctgc ccttcttcgt cgtctcgaac ttgtgctgcg cggcgtcgaa
     7801 gatcttgtcc cagccgccga gctgatgcgg gatccacagc acggccgcga tgatcacgat
     7861 gtagatgagc gtgtccttga cgaacgcgat cagcgccggc gcgcgaaggc ccgattgata
     7921 cgtatacgca gcgaggatcg cgaacgcgat gatgagcggc aggtggcgcg cgacgaagct
     7981 gtcgccggtg agcccgatcg tctcgagcac cgcttcgata ccgacgagct gcagcgcgat
     8041 gtacggcatc gtcgcgacaa tgccggtcac cgccacgagc agcgcgagcg tcggcgagcc
     8101 gaaccgcgca cgcacgaagt ctgccggcgt gacaaaacca tgcttgtgcg agaccgacca
     8161 cagccggatg agcggcagaa acacgagcgg atacgtgatg accgtgtacg gcatcgcgaa
     8221 gaagccagcc gcgcccatcg caaacatcaa cgccggcacc gcgacgaagg tgtacgccgt
     8281 atagagatca ccgccgatca gaaaccaggt cacccaaccg ccgaagctgc ggccgccgag
     8341 accccactcg tcgaggtgct ccatcgtctt gggcgcgcgc cacttcgccg cgacaaagcc
     8401 catgatcgcg acgaccgcga acaggacgcc catgatcacg agctcggtcc agttaacgtt
     8461 cgtcatggct ttcgatgacg cgtcatgcgg tagacgatcg tcgtgcacaa cacgccgagg
     8521 ccgacgaacg cgatttgcag ccaataatac cgagggaagc cgaacagcct cggctccgcg
     8581 tcgttgtaga ggaacgtcaa gagcggcacg acaatcggaa ccagcaagag ccagttccac
     8641 ggattgcgat cgctgtgctc gtcgtcgcgc gtctccggct tctcgctcac ggtcgcatct
     8701 tagcgaggag cttcctgatc ttcgcggttt ccgcgcgctc gatcgggatc ttgccggtcg
     8761 cgtccttgat gctcgggtcg gcaccggcgg cgagcaaccg ctcgatggcc tcggcgcact
     8821 tgaggtcgac tgcaacgtgg agaggcgttc gtccaatggc accggcgcgg ttcaccagat
     8881 gaggcgcgtg ctccagcaac cggtcgagca cgggcagccg gaccttgtgc tgcggcgcga
     8941 agtgaatcgg ccgatcgccg acgaagtccg cggcatcagc atttgcgccc cccgcgagca
     9001 gcatgttgac catctcgagg tcgttcacca tgaccgcgca atgcagtgcc gttcggtggt
     9061 gcggagcttc gtttgaatcg actggcacgc tgtcggcgag catcttgcga acgaggtccc
     9121 tcttcttcgc aaaggccgcc tcggcgagcg tgagcgccac agcgccagac tagcgcgtcg
     9181 acatcgtgac gagatacttc tcgatcagct tctgctggga gaaatcgagg ctcgcgcgcg
     9241 acgccatctc gtcgagcttc tgcggccaat ccgccgccgt atgatcgttc ggcatcggcg
     9301 cgcgatggca gttcgtgcat ttgccgatga ggagcttgcg cccctgctgg agctcggcga
     9361 gctcgacgtt gccgcgctgc gcgtcggtcg ccgtcaccat cggtggctga cgtggcgcgc
     9421 accctgcccc gctggcgatg accgccgcga cgccgagcac gaatgcgcgc gcgttccgca
     9481 tgactacagc tcgactccca cgatgaggcg gccgctcagc gcaggcgctt cgtcggtgag
     9541 cccgaaggcc gccgtgaacg tgaaccagaa gttgccgctc ggcgcgatcg cgagcgcagg
     9601 tccgatcgac gcgccgactt cttcttcctc gatctcgccg tacgtctcga cgccgacgtt
     9661 taccttcgga tgcacttcgt agctggcgcc gagtgcccag ccgaattccg gctcggtctc
     9721 gggcgcgttc ttgccgagca tgatctcggc gatgacgttg agcgcgacgg tcaccttgtc
     9781 gaagtcgcgc gcgaagatgc ccttcgcctc gacctcgtag atgctctcgc cgaacacctt
     9841 cacgagctcg ccatacgcga ggatgtcgac cggaagctcg ccgcggtccg cgaaccggta
     9901 gcgcgtctcg agcttcatct ccgcgaggtg aaatggctcg ctctcggtcg cgacgcctgc
     9961 aaccgtgccg cccgcggtct gatcgaacac ggtgtagaac gcgaggtccc agtgatcggt
    10021 caggccatgc tcgatctcga ggatgtgctc gagcgcctgc ggcgtcgtgt tgtcccaggt
    10081 ctgccgcgat tcggtgtgcc agagctcgag tgcggtgtgc ccttccggga ccgtcttgta
    10141 ctcgtaggtg ctggtgaaca tgcggcggtc ggcgtgggcg gtggagtcga ggaacgtcgt
    10201 cgcggcgaga gcgccgccga caaccaatag tctagctgaa aagctcattt ctgagattga
    10261 aaatagattt caatttctga aattgcaagc tcctcctaga caaccttcca accgcacgtg
    10321 attacatcga aatcgagccc ttgcggaagt cggtcttgcg caggtggatg ttgatacaca
    10381 ccgggcggcc gccctttgcg atcgctttgg cttccgcgag cgtggagtcg acgcgcgctg
    10441 gatccgtgag caagaggccg acgccgccgt aaccttcggc gaccttgtgg tagtcgctgc
    10501 gtcgcagatc cgtgccgagc gacgtgccga ggatctcgac ttgctcgcgc gcgatctgca
    10561 tccacgacgc gtcgttgccg atcagcgcga ttggcgcgac gccgtggcga acgtacgtgt
    10621 cgaactccgc gagcgtgtac gccgacgagc cgtcgcccca cacgagccac acctcgcggc
    10681 ctgggcgaac gagcgatgcg ccgagcgcga agccgccgcc gacgccgagc gtgccgaaca
    10741 cgccgggatc gagccacgac agcggcgcgc gcgggcgaac gatgtagctc gcagttgcga
    10801 cgaagtcgcc gccatcgacg acgaggaccg catcgtccgc catgtgctcg tcgaggcgcg
    10861 tgaagaagtg gatcggatcg acgagctcgc ctttgggcgc cgacttcgct gtgatctcgc
    10921 tgtcgcgtga tgcttcgcga tcgcgcagcg tcgagaccca atcggtccat gcgccagcgc
    10981 caccgcccgc gcgctcggcg agtgcgacga ggaaatcgcc ggcgtgcatc tcgatcgcga
    11041 tgtctgggcg gcgattcttg cgcagctcgt gcgtcgacag gttcgcggcg ataagcgtgc
    11101 cctgcttgtt gatgccgcgg ccatagccga ggcggaagtc gaacgggaag ccgcacacga
    11161 tcacgcagtc ggcttctttg agcgctgcgc cgcggttgtg gcggaactgc agctcgtgcg
    11221 tgcggccgag taacccacgc gcaacgcccg cgagaaacac gggtgcaccg agctggccga
    11281 tcgcccgcgc gagacgaccc gggtccttca cgccaacgag cgtctgactg ccgacgacaa
    11341 tcaccggctt cttcgccgtg cgcagtgcct cggccgctct gtcgatcagc ccatcgccac
    11401 ggagcttggg cagcttcatc ggcccgagat cgatctcggg catcgcaggc gcgcggaact
    11461 gccgatacag gtgaccgcga atgtagagct cgagcgcctt cgcgccgatg cccttgcctt
    11521 tgcctagccc cgcttctttc tcgtaccaac cgcggacgat ctcctcggag tagagcagat
    11581 cgacgggcac ctcgacgaac accggaccgg gcacaccctc ggtggcgatc tgaatcgcgc
    11641 gctcgaccgt atgcacgaga gacggcaccg tcttcaccgt cgtcgcccac ttcacgagcg
    11701 gcttcatcaa ttcgatctgg tcgatgtcct gcagcgcgcc gcggcccttg agcagcgtcg
    11761 ctgtcgcgcc gccgaacagc aggatcggcg actgcgccat ctgcgcgttc ttgatcgccg
    11821 tgatcgtgtt cgtgacgccc ggccccgccg tgaccgcggc aacgccgatc gttcccgtca
    11881 tgcgcgcgac cgcatcggcg gcgaacacgg cgttgccctc atcgcgaacg tcgacgactt
    11941 tgatcccgcg agcttgagcc ccggtaagga tcggcgagat gtgaccgccg cagagcgtga
    12001 acaggtgcgt cacaccatga cgcgcaagga catccgcgat caggtctccg ccatgttgag
    12061 gcatggcgcg tactatagtc gggctgatcc agcctgagcc gaagagatca tgtcgaatct
    12121 cgttctctat gcgattccgg cgttcctagc gctgatcatc ctcgaagcgt tctgggcacg
    12181 ccgccacccc gaggacatcg tcggctacga gcggcgcgac accgtcgcga gcatctcgat
    12241 gggcgccctc aacgtcgtcg tctccgccgt cgcgaagctt gcatcgatcc cgttcttcgc
    12301 gtggctctac acgcatcgca tcgcggacct cgggcaaccc gcggcgtggt ggtcgtggct
    12361 cctcctcttg gtcgccgaag acttttgcta ctactggttc caccgctcgc atcacgaagt
    12421 gcgcctgctc tgggccgcgc acgtcaatca ccactcgagc cagcactaca atctctccac
    12481 cgcgctccgc caagcgctgc tcaccccggt gacgggcccg atcttctggg cgccgctcgc
    12541 cctcctcggc ttcccgcccg tcatgatcct gaccgcgcag gcgtggtccc tgctctacca
    12601 attctggctc cacaccgaag cgatcaagac cctcggcccg ctcgagtgga tcatgaacac
    12661 gccgtcgcac caccgcgtgc atcacggcaa gaacgtcgtc tatctagatc gcaatcacgc
    12721 cggcatcttc atcatctggg atcgcctgtt cggcacgttt caaaaagaag aagagcgcgt
    12781 gatctacggc ctcacgaaag acatccacac cttcaacgtc ttccagatcg gcttccacga
    12841 gctcgtcgca atcacacgcg acgtcgcgcg ctcccgccgc ctcggcgcca agctcggcta
    12901 catcttcgcg ccgcccggct ggagtcacga cggctcgacg aagaccgcgg gccagctcca
    12961 caaagagctc cgcgccccgc gttcctctct atagagatgc tttgagcagc aacaatcggt
    13021 ctgaccacta agggacggac gcaacttccg caacctccgc aacctccagg gacggagcag
    13081 tggcctgacc actaagggac ggacgcaact tccgcaacct ccgcaacctc cagggacgga
    13141 gcagtggcct gaccactaag ggacggacgc aacttccgca acctccgggg acggagcagt
    13201 ggcccgacca ctaagggacg gacgcaactt ccgcaaccgg ttgtttcaca ctccgcaacc
    13261 tccagggacg gagcagtcac gacggccgct tgaacacgag cgcgtcgggc gtcgccgcaa
    13321 ccttccgctc caggtagtga tcgagcttgt cgagcaggta ccaggtgccg agcggattgg
    13381 cgtgcaggcc atccatcgcc atgagctcgg ccgcttcgac ggtctcgccg gtcggcagct
    13441 tcaccttcgc gcccgtgcgc agcggcgcgg tccactcgac gagcggcacg agcacgacat
    13501 gatccttctt cgcccatgcc gcgatcgcct tgttcgccgc atcgagccgc gcctgcgtcg
    13561 ggatcgcatc cttcggcagc atcatctcgc tcgccgtcgt gatcagcgga atgtcgccga
    13621 gcacgatcca gactcccgcg gcgcgcagct tctcgagctc cgcgagtgaa tcggcgagcg
    13681 ccttgtcgtg ccacgccgta tcgaagccgc cgtagacgtc ccagaacaag aggtcgacgg
    13741 cgatcaggat cgatggcttg aacgcgcgcg cttcatcgag ctgccggtgc gtgtcgccga
    13801 tcggatcctt gaacagcatg acgttcgcac tcgactgcac cgcggaatgc ggcgccgccg
    13861 cggtgaacgc atcgccgaat ggcgcgccgc cgaacccggc tgacacgctc gcgccgacaa
    13921 tggcgatccg cgcgaacgga cccgtcgcag ttgcggcgct ccgtccctcg ccggttgcgg
    13981 aagttgcggc gctccgtccc tcgccggttg cggaagttgc ggtgctccgt ccctcgccgg
    14041 ttgcggaagt tgcgtccgtc ccttgctccg tccctcgcgg ttgcggaggt tgcggaagtt
    14101 gcgtccgtcc cttatcggga tacccagggg tgttggaggc tcccgtccca ggtggggtgg
    14161 tgacggcgac tcggtccctg acgggctctg tccggcaagc ggacgccagg gttaggagac
    14221 ctagccacgc gattggccgg atcgagaggc gttggcggga tctcaagttg tgattcttgc
    14281 acttcatgga ctcgtgtttt cgcggggttg ggagccgagg tcacttgtgc ccagctgggg
    14341 gctgtctcct catggccaat gtaggcgcta gatccctacc tctgaggaaa ttcatacagt
    14401 tggaaaaggt gtacaggctc tgcacagtgg tacgagacgt gctgtacgat ggctcccatg
    14461 ctgggtggtc tcactcctaa gacgtcgctg aagtctcggc gcgccatgtt ggataccctc
    14521 atgcggagcc tgcccatggt tcgcctgtcg ctgggcgcgc ttgcagcgct gtctattatg
    14581 ggctgcagcg gcctcattga tagcggcggt gacgacagct cggttactcc cgaggagaag
    14641 tcggctcgcg cggcctatat gaacaaggcc aagcccatct ttgatgtggc atgcgccagc
    14701 tgccattccg gttcagatcc gacagttgcg ttcctctctg gcgccgaccc gatggcccag
    14761 cgcgcgacgc tcctcggctt tgacccccag gtcgtgaacc tggcggccgc cacgtcttct
    14821 cgcctgctta cgaagggcgc gcactccggc ccggcactgc tcgcgtcgca agcctctgag
    14881 ctccttgagt ggatccaggc ggaacgtgat gcggctaacg tcagtggggg tgtggacccc
    14941 ggcctggaga ctgcgctctt caccccccag ctctgcacga acacgaacgc ggtccccggc
    15001 gaccccgact gcccgatcaa catcgttccg ctcgatggcc tcgttcaggg ttgggccggc
    15061 gcacgcatca agttcgtcgc gcagccgctc tcgtcggcgc tgtacgtcac caacctctcg
    15121 ctcgagggcg gcgcggatgg cgtgtacatc gagcacccgc tcttcgtctc gtggccgctg
    15181 acggaaggcg cgcagccgat tcccgatcag ctcgatcgct tcttcaacgt gaagatgaac
    15241 ctgatgaccg gctcgatggc gacgcccatc cagggcggca ccgcagcgtt catcaacttc
    15301 atgccgaaca acaagctgac gatcaacttc aaggtcgtcg acaagttccg tcccgaggac
    15361 ggcggcatgg gcggcatgac gatggcgtcg ggctgcaagc agctcgcgtc gttcaagacg
    15421 aatgcgcgcg gcccgctgca gacgaactgc gcgagctgcc acgcggcggc cgccaacacg
    15481 aacgcgcgcg gcgcgatgga catcaccggc atcgccgcaa tggacgacgc cacgctgcag
    15541 acggtctgca accaagcacg cacgcgcgtg aacttcacgg acacgaacca gagcgggttc
    15601 ttcatcgcac cgaacccggc gcaggcaacc aatcacccgt tcaagttcgg aaacgttcaa
    15661 gcgaacttcg acaactttaa aacggccgtg gatgtgtggg tccaggccga gaaaacgtcc
    15721 cagtagtttc cccaaaggag ccccatgaag aggatctcag cactactcct ggccgccgca
    15781 tcgttcgcgg tcccggcctg cagctccgaa caaggagatg acagcgtggg ctctggttcc
    15841 ggatccggca gcggatctga aaccggagat gaatgggacc aagcgctcag tcagcgcgag
    15901 tacgactaca acgcggcgtt gcgcattgct gcgcttcgct tgacgggcgc gctgccgacg
    15961 atggccgaga tccaagagat cgcgtccgcg ccggacaacg cgacgaagaa gggcctctac
    16021 gaggcgcgca tccagcagta catgtctcgc cccgagttcg ggaagcagat gatgtacttc
    16081 tggcgcgaca ccttcaaaat gggcgagacc gcgatgctcg acaccgcgcc ggcgtttgcg
    16141 accggcctgt cgatcaacaa cggctcgtac atggacatgt tcacggcgag ctcgggcaac
    16201 tgcccgacgt tcgacatgac cgcgggcacg gtggcttcgg ccgagtgtat gaacggcgga
    16261 ccgaaagccg gtctgctcac gaaccccggc attcagtcgc actacttcgg aaacctcggt
    16321 ttccgtcgct cgcgcttctt ccaggagacc ttcgtctgca cgaagttccc ggcggaagtc
    16381 ggaaccgcgc ccgtcgatgt cggcggcgcc gcgccctaca ccggcgtgtg gccgttccag
    16441 tcgatcgcga gcccgaccaa cggtggcggt cgcatcaact tccaagacac gtcggcagtc
    16501 atctgcgcga actgccactc gacgctgaac cacctcacgc cgctgttcgc gtactacgac
    16561 gatcagggcg cctatcagac ggccatctcg gtgccgacgc cgctcgacgg cgcgccgctc
    16621 gcgaagatga ccgactacct cccggctggc gaaaccaccg cgtggcgtct caacgttcct
    16681 gctgctgacc tcccggccct cggccaggcg atggcggctg acgcggacgt cgccgaatgt
    16741 ggtgtcgctc gcatgtggaa ctgggcgctc ggcaagacgg acatcgtcga cacgctccag
    16801 gaagttccat ctgaaaccat cgcggctcag gtcgatgcct tcaaagccaa tggcttcaag
    16861 atgaaggaca tgatcttcgc cgtctacacc agcgacgact tcgtcaagtt ctagggagga
    16921 agaccatgaa gaccatcgga tataccctcg ctatcctggc ctcgtcggtc ttggcttccg
    16981 cctgcaccga cgacaccacc acgacgaatg agccgcccac cggctcgacg tcaggcaacg
    17041 aagacacgac gttcgatcac gacaacggcg attacatctc gccgtgggag ctgctcgacc
    17101 gtctgcaaaa ggaaggtcct ccgcgttaca cgtcgaaggt ccactcgtgc tcgaaggtcc
    17161 gttacaagac cctcggcacc gtcctcacga acgtcggtat gaacccggcg aacgcgacgg
    17221 cgctctcggc cggtaacctc tacacgaccg gcttcaacgc gctcggcggt ccgaactacg
    17281 cgaaccgcat ccgcgagaac atctcgatca cgacctcggg cgcctcgcgc gagttcgaca
    17341 tcttcgcggc tggcgcgcag gaaacgatcg acgcgttcgc caaccagacc ggcatcgccg
    17401 gcacgggcgg tcgctgcgct ggcatcacgc tgttcgatgc gagcaacaac tgccgccccg
    17461 acggcatcac gtgtctcacc ggtgtgccgg cgactgcgct gcacctcgac ttctgcaaca
    17521 aaaccgtctc gatggcatcg gacgttaccg tcggcaagcg ccttgccgtc gcggacatgt
    17581 tggccgcggc ttacacctgc gagtgaggag acccaccatg gccaaatcac ctatctccaa
    17641 gcgtgacgaa gagcgtgaga ttacccgccg cgctcttatc aagtggagcg tcgcagcggg
    17701 cgcagctctc ggcgtttcgc gcgcgaaggt cttcgagatc ctcgagcgta cggccggacg
    17761 cgacgtagcg ttcgccgctg ccgaaaaccc gaccactcgc tcggtccaca tcatcgccgg
    17821 caacggcggt aacgcgtggt tccagctcct gtggccgcac aacgacatcg ccgctgcgaa
    17881 caacacgacg ttcgcgtggc atcgtccggg cgagtcgcgc ctggtcgccg gcacggacaa
    17941 gccgctcacg gttggtccgg acacgccgtt cgccgacatg ccggcagcgc gtcagatgac
    18001 gtgcttccag atgggtaaca acgagaccca taccggcgcg ccgacctcgg tgacgagcct
    18061 caacggcaac aacatcatct cggttgcgag cgcgctgcag tcggccgctc cgtcggtcat
    18121 cccgatgatc gcaatcggtg gcgtcgatgt cggcaccgca cccggtgcgg cgcgtcctgc
    18181 cgctgtcggc aacgcggatg gtgtcgtcgg tctcttcaac tcggcggcgt cgcgcgccgg
    18241 cggtctgctc tcgaaagcgc aggacgccac gctgtacaag gcgcactacg acgccttcgc
    18301 gcagctcaac cgcgcgcaga accgctcgac gcagaagacg gcgtactcga cggcgtcgaa
    18361 cgcggcgcag ttccttggaa cgaacctcgc ggcgaagctg cagatcgtcc aagcggacct
    18421 cgaccgctac ggcatcgacg gcacgacgcg cgcgaacgtt cgcgacatcg gccgcgccct
    18481 catcgtttcg gtgaaggcgt tcaagatggg cctcaccaac tcggtgtcgc tgccggccat
    18541 gcgcgacgat ccgcacggcg cattcgccga cggctcggcc atgtcggttc cgccgatgct
    18601 caagaaggtc ttcgacggct tcatgaacga cctcaagatc accgtcgacg acaacaccgg
    18661 caaggtcctc gcggatgaca cggtcatcac gatcaccggt gacacgacga agaacccgct
    18721 gcagcgtggc ggttggccgg atggtacgcc gaacaactcg aacgtcatgt gggtctactc
    18781 ggccggtcac ctgatgagcg gctggttcgg cggcacgctc cgcaacggca cggcgcaagg
    18841 cttcgacgcc aacggcgcga cggcggcata caacggtgca aacaccgcgc gtatggccct
    18901 cgcctcggtc gcgtacgcga ttgccaagcg tgatgaccgc gcgatcaccg cgttcgcgaa
    18961 cggcatcaag gtcggcggcg tcttcggtcg cctcaaagac ctctaagctt cgcccacgag
    19021 cgacgctcac cggcgccctg ccctcaccgg ctcggcgccg tttcgttttt tcggcgccga
    19081 tcggctacga tcgacgcggt gcgcgtgtgg ctcgtcatcg ttctgctgtt cgctgtggct
    19141 tgcgagaaga agcccgcggg tagcggcacg accggcagtg cgagctcgcc gctgaacgac
    19201 gaggccgcgc gcgagcaagc gcgcttcgat gccgagcggc gtccggacaa agtcgtcgag
    19261 gcgctggcga tcaaaccggg ctcggtcgtc gccgacgtcg gcgcgggctc gggtctactc
    19321 accgtgcatc tcgcacgcgc cgtggcacca aacggcaagg tcgtcgcgac cgatgtcgac
    19381 ggcggcgtgc tcgggtttct caatcagcgc ctcaagtcac aaaacctcgc gaatgtcgtc
    19441 gagacgcgcg ttgtggccgc cgacacaccg ggcctcgagc cagcaacgta cgacgcgatc
    19501 ttgctcgcgg aagtcgacca ctatttcaca gaccccgcgg cctggttgcg cgaagctggg
    19561 aaggcgctaa agcccgaagg acggatcgtg atctcgaacc gcatctacca ccgcgcgcag
    19621 acgatggcgt ctgcgaaaaa agcgggcctg gtgttgctga ccgagacgac gcccgtgccg
    19681 actcacttca tcgctgtgtt caccaccgag gccaagaaat gaagctctcc cctcttctgc
    19741 tgctcgcgct cgctgcatgc cccggtgacg acggcgatgg cggcaatgcc atcacgctgt
    19801 ggctcgcacc ggatggccgc gagaccgagg tcaagctcgt cgagtccgaa ccgactccgt
    19861 attgagcgcc aggcgtgggg gccggtggct ctcaggaagc agaatactcg ctgaagggaa
    19921 gcgtccctgc cggcacgtat cactgcgtcc tcgattcgat catcattcgc cccgtcgacg
    19981 tcacgttcga tctcatctgg cggcgcggca cgacggacat gccgctcgcg acgtggatgc
    20041 agcacttcga gccgctgccc ggaagcttcg acgcgcaggc gtacgagatc gatcaagccg
    20101 cgcccgcgat cgacttcgcc gccggcgatc agctgatctt ccgctacagc ggtgcgaaca
    20161 cgacgtcgtc cgaggcgtac atcccgaacg gcgacggttc gctctcgcac ggccgtatcc
    20221 cgaacatcac gctccccaag tagcttcaga ccacgcgccg tcgaaccgcg ttataaggcg
    20281 tgtatgaagg tccttggact tgtcgtggcc atggtggtgg ccgctgcgcc ggcgtttgcg
    20341 ggcgagcagc gagcgtgggt gaaggaggtc ggcaacctcg acacgtggaa gcactattcg
    20401 aagaccgtcg gcactgacga gttcggcaag gccgtgatcg acgtgaagac gaacgagatc
    20461 tatttcttcg acgtcaacct gttcaacatc cacgcggact tcgtgctcgg cgtcctgctg
    20521 aagcaaccgt ggaccgcgac gaacgtgcgc gagtacaaca agaactacga gcgcgacaag
    20581 ccgcgcttca tcctctgcta cttgactcac catctgaagg tcgacaagtg gacgttctcg
    20641 ttctgggagg gcgacaagat cggtcccgac gacgtgatga aggtcagcaa gcgattcgag
    20701 cagacgttct tcatgaagaa gctgccgttt cggcctgact cgccgctgca gatgaaggtc
    20761 gcggccgacg tgaagaagaa gggcctcgag atcatcacga acgacgacat ctacaaggcg
    20821 gctgactttc aggcgttcaa caagggcaag accgtcggca ggctgcgcgt cgttcccgtc
    20881 ggcaccgcgt tcgaatccct gacgttcgat cgccacgaca tcgtgctcct gcaagagagc
    20941 tatcccgaca tcacgcccgt cgccggcatc atcgcgacgc agttctcgac gccgctgtcg
    21001 cacgtcaacc tgcgcgcggg tgcgtggggc attccgaacg ccggcgacaa gaaggcgcgc
    21061 gagaagttcg gcaagctcga cggcaaagtc gtctacttcg aggtgaccga tacgaaggac
    21121 gtcatgcgcg aggccacgaa agaggaggcc aagcagctcg agcaatcgat ctacagcgcg
    21181 aagcacgtcg acttgccgga ggcagacatc acgacgaagc gcatggcgat gctgacgcgg
    21241 atgcgcgcga aggacgtgct gcgctacggc acgaaggcgt cgaacctcgg cgagatcgtg
    21301 accgcgaacc tcgacggcgt gaacgtgccg cctggctttg gcgtgccgtt cgcgttctac
    21361 gtgcagcacc tgcagaagaa cgggctcgac aagaagatcg aggcgatgct cgcggacaag
    21421 aagttcgcgg tcgatccggc gtggcgcaag acggcgctcg aggagatccg caaggcgatc
    21481 accgatgcgc cgatcgacgc cgacacgctc gacatgatct acaagcgcgt gcgcatcaag
    21541 ctcggcggca agggcgtgtt cgtgcgcagc tcgagcacca gtgaagacct gccggggttc
    21601 aacggcgcgg gtctgtacga cacggttgga aacgtgcgcg gcaagaaggc gctcggtgat
    21661 gcgctcaagg tcgtgtgggc ctcgctgtgg aaccttcgcg ccgtcgacga gcgcagcgcg
    21721 ttcggcatcg atcatcggca ggcgtacatg ggcgtgctcg tccaggtcgg cgtgagcgcg
    21781 accgcggccg gcgtgctcgt cacgaagaac ctgtgggatc cgggcgacga ccacagcttc
    21841 acgatcaaca gcaagtgggg cctcggcgag cgcgtcgtgc agggcgtgaa gatgcccgag
    21901 cagatcgtgt tcgatccgac gaacgacggc acgaagatca tctcgcgcgc ggacgacccc
    21961 gtgatgctcg tgttcgacga caaaggcggc gtgaaggagc tgccggttcc ggcgggcgcg
    22021 ggcgtgatcc tcaccgagga acgtgcgaag aagctgtcgc agatggttca gaagttcatc
    22081 ccggtgttca cgcacggcca gccgctcgat gtcgagtggg tcctcgaagg cgaggaagtg
    22141 tggatcgtgc aggcccggcc attcgtgggg tcctgatgcg tcgcgctctc gttctgatcg
    22201 ccctggccgc ttgcgagctc cagcctgcgc cgaagaagca gccagttccc gtcgaggtgg
    22261 tgacgccacc gccgcagccg gttcaagcgc ccgtcgtcgt cgatgcgggt gctgcagcgt
    22321 ccgatgccga ggctccgccg ccgatcattc aagcgtcggc ggactgcatc aacgtcgccg
    22381 cgcacgtcgt gcaggtgttc atcagcacgg cgcgtgatca ggctcaggcc gcggtctacg
    22441 agcaggagcg catgaagatg acgcgcgcga ctgccgaggc gtgtgagaag cagcagtggt
    22501 cgaccgcggc gcagaagtgc tatctcgcgt cgaagaatcc cgccgaaatc aaagcgtgcg
    22561 agaacaagtt cccgccgccg ccgcccgaga gagagggcaa tggctctgcg gtcgtgaagc
    22621 ccacgaacga gcctggcggc cccgcagtgc cgcctcgcta gaagatccag ggaatcgaga
    22681 cgagcacggc gaaccgcagg acgcggcccg tgaagccgat caggcagaac agcgtgaagt
    22741 tgatcttcag cgcgccggcc gcaaacgtcg tcagatagag cggcggcaag ccgagtgatg
    22801 cggacgccgc gtacacgagg tacggctgct tctcccagcg cgtcagcatc ccgcgcgcgc
    22861 gatcgatgcg tgccttccac ttgccggtgg gcagctcgaa catgcccatg cccgcgtagt
    22921 agaggatgac cttcgcgatc atctggccga gcgccgcgag gatgatgatg gccggcagct
    22981 gctcgggccg cttcaccacc cagatcgaaa tgccgacgag atagacctca gcattgatga
    23041 gcggaatgat gcccgcgaga aagcagacca cgagcgtgcc ggcatagagg ccgagcgtct
    23101 gcagcagatc gtgcgtcatc cccggcatcc atggtgaccg gcactcaccg gggtggcaac
    23161 caaacggatc ggcaaaagct gtctcacctc agcgagtccc ttagctggtt cgaccaacca
    23221 tgccccagac gaagtgcacg atgacgagcg cccaaatgcc gaagtagagc ggcaggaagc
    23281 gtttacggag ctcgagatat tcggggtgga ggatcggctt tcctgcgaac ttgccgcgcg
    23341 gtttgttcgg aaagaagaac gtcttgtagg tctcgatcgc catcacggtg aagccttcgg
    23401 cggcgatcgc gatgatcgtc cacgacgacg tgaactggca gaccacgaat gcccagctcg
    23461 cagcgacgcc cggactgcgg aagaacttga acgtctcgaa gccttcgatc ggtgcgtcct
    23521 tccacgcgcc gccgaaagcc gagatccaac cgaagaagct tccgacggtg atgacgacga
    23581 gccacatcgg ccacgtcgtg ccccagctct tctcgagcgc gcgaatgccg tagaacacgc
    23641 cgatgcacgc gatcgcgacg aggatgccga tcgagatccg cttcttcgcg tccttcatcg
    23701 gcttgccgaa caggccgaac tgcatcggga tgaagtactt gctctggtcc tcttcgcgaa
    23761 tgaagtattt ccagaactcg gtcgctgcgc gctccaggca gtagatgagg ccgaagtaga
    23821 cgaagcgctt gccggcgtca tgcgcgaggt cccactggac gaagtggtgc gtgacgccgc
    23881 cgatgatcga agcgacgatc gcggagcgga agtacttggg ccaggtgaag ccctcgtgcg
    23941 gggcgtcctt gtacatgccc cacgtgccca tgtggaggcc gacgagggtg ccggtgagaa
    24001 tggcgatggc gaggttcacg aaactcctgc ggtggcgtac gcctcttcaa cgagctcgag
    24061 gtcgcgacgc gcgcgcgcga gggtcatagc gggttctgtc cccgacttcc aggcctggac
    24121 gaagtctgcc cacatcgcgt tgtagcccgc gagatcacgc agcccgggga cgtaaaaccg
    24181 cgtctttttg ccatgtaaga acacccacag gccgttcgtc tcgaacgtga cggagccggc
    24241 acggccgtag atcttcgaga gccgaaggcc cttcgccgtc gagggaacct cccacgaata
    24301 ggcgagcgag ccgacgaaac cctcggcgta gtcgaacgtg acgagcatcg agcgctcggg
    24361 cttcgcgccg ttgctcgggt gcaggccgcg cgccccgcgc acggtgccga ggctcgacat
    24421 gaaatcgatc cagtggatgc cgccttcgta gagcgcgccg tagtcggtgc gccacgcgtc
    24481 ggacttctgc agcttgatcg cgttgacgtg cgcgaacaat ggctcgccga tgaggccgtc
    24541 cgcgaggacc tggcgaatgc ggcgcagcag cggcttgtat tgatagttct cggcgacgaa
    24601 cacctgcttg ttcgcggcgg ccgcggcctc ggcgatctgc gtgaagtccg acgcgcgcgg
    24661 cacgggtggt ttctcgagca cgacgtgctt tcccgcagcg agcgcggcga gcgcgagcgg
    24721 caggtgcgaa tccggcggcg tgacgatcgc gacggcgtcg acatcgggcg atgcgatcgc
    24781 ttcttcgtac gtgccgtgcg ccgcaccatc gagctcgcgt gcgaatgctt gcgcgcgttg
    24841 cgggtcgcgg ctcgagaagc cgacttcgac atcggctttg tgcgcgcgca atcgcttcgc
    24901 atgcgccttc gcgatcttgc cgcagccgag gaacgcgagc cgaatcacga gtgcgtcatc
    24961 gccgttcccg ctcgcagcgc gttcgcagcg atcgtgaggc tcgggttgac gccggcggag
    25021 agcggcagcg cgctgccgtc cgtgacccac agattgtcga cgccgcggaa gcgacagtgc
    25081 tcgtcgagcg gcgacgtcgc tggatcgacg cccatgcgca gcgtgccgac ggcgtgcgag
    25141 aacgtcttca cgcgatgggt gtaagagacc acggcgccca tcttagagag gatttcacgg
    25201 gcgcgccgcg cgaggacgtc gacggcgaga ccgtctcgcg cgctgtacga cgtgccgatc
    25261 acgagctgcg gaaggtcgta ctgatcgcgg acgtcccaat cgagcgcgac accgttgtcc
    25321 aggcgcggtt gatcctcggc gatcgcgagc aggccggtca gttgctcggt gaacgtaccg
    25381 agcaaggacc tcgtcccgat cggtaggtgc gcgcgcacga gctccttcgg tggcgtcatc
    25441 acttgctgca cgccgccgag cttcgtgagg tgcccgcctt cgtgatcgct cggatcgccg
    25501 aagtaccagt cgtggatcgc gagctgcttg tgaaaccggc gatgcggatc cgggcgctcc
    25561 gggaacacgc cgaacaccat cgcgttgtcg tgccgcatca ggtagcgacc gacggcgccg
    25621 cgtgccggag agagctcgtc gaggcgcgac gacaacacga gatgcggcga gccgagcgca
    25681 cctgcggcga gcacgtattg atacgcctgc agctcgacgc gctcaccggt gtctcgccgc
    25741 aggacgtgga gtcgcttgat cgtggtgtcg ttcgtctcga ggcggagagc gacggtgttc
    25801 gtccacagcg tcatcccgcg cgcgacgaga ggcgcgatca tcgtcgtcgc gacgtcgttc
    25861 ttcgcttcga tcgcgcacgc gaacgtgtcg cacgtgcggc aggcaacgca tgctttgcgc
    25921 gtgtcgttca ccgcgtgatt gatcgcgagc ggcagatgaa acggcgacag ccccatgtgg
    25981 accgcggtct cggcgacgcg catcgagagc ggcgcgagct ccgccggagg ctgcgggaac
    26041 ggagtcgagc gcggaggcgc cgtcggatct cccgcgtcgc tgcccgacac accgaggagg
    26101 cgctctgcct ccgcatactc cgcctcgatc tcgtcgtagc gatacggaaa cgctgcgccc
    26161 gaatcggcga cgacatcgcg atcgccagtg aagtcttgct cgcgaaagcg aaacgacacg
    26221 cagccgtaat agacagacgg accgccgacg caggtcaccg cgccgatgta ctggccatgc
    26281 ccgccgcgct cgcagcgata cggaatgtcg cgcgcgtagc tcggcgtcag ctcgaacgaa
    26341 gcctcgggcc tccacgccgc ctcaccgcgc gagacccaat cgccacgctc gatcatcagg
    26401 acgcgcgcgc cggcctcgac gaggcgatgc gcaaccatcg cgccgccaaa accgctgccg
    26461 acgatgactg cgtcccactt caaaggtgag ggaccctagc attcacccag cgcgactggc
    26521 gaggggcgat cggtagctcg cgtcctcggc gcggcgtgcg cgttcgccca gtgctcgaca
    26581 cacagacgag gcaagccccg gcacatcatc gacaagctct tcgaagtcga cgcgctcgag
    26641 cctgatgagg cgcgcgctcc ggatcacagt gaggtcggcc gccgctgccg ccggcgcgac
    26701 gcacgcgagc tcgtcgacga cctctccctt cgcgatccgc cgatcgccga tctgcagctc
    26761 gccgtcctcg acgacgatca gcgcatcgat cggatcgcca ccgctgacga ccacgctgcc
    26821 ttctcgcgca ttcatccaac gcgcgcgctc cgcaacgtcg gcgagctgtc gcgtcgagag
    26881 cccagcgagc agcggcacac ggcccagcgc aatcagtgtc tcgacgcgac tcggcatatc
    26941 actcgctcct tcgtcgacgg gtgtgtcggc ggtgggttcg gtcatcttcg tgaggcggcg
    27001 cagcagctcg gtcgggctcg cggcgagggc ctctgcttgc gctgccttcg cgatcgtctc
    27061 gcgatgcgcg ctgcgatcca cggctcccaa gacatgcagc acgagcgcgc gcgaaagtcg
    27121 atcgcgcccc gcgagctccg ctttcacgac ggcgtcgcgg cttggtagct cgccacccgc
    27181 gcgcacgaat gccgcctttc gatcgctcgg cgtcacgtca tcgacggcct cgacgagccg
    27241 cgtgacgagc gcgcgcggca tggcagcttc gatcacggcc agcgccctcg cccgctcctg
    27301 ccctgctcgc gcgtgctgcc acgcgaccgc cgcccgcgaa atagcggtcg accgccgccg
    27361 cgcggcgacg agcagcagga cggtgtgcgc gatctcgcgg agccgctcgt cgaggcgtcg
    27421 cgcgacgagc tcgtcgcccg cctgcgtaaa gacggtgatc gcggtcgcgg tctgctcgag
    27481 cgcgtcgagc tccgcatcga cgaggcgatc gatcgtcgcg ggcgtaacgg gaagatcggc
    27541 gagcaatgcc gccgctcgat cgcgtgcgcg gcggcgtccc cacgcaaccg tcgcgagcag
    27601 ctcgccggcc gctgccggtc caagcccgac gagcgcagcc tccgctgcaa gccggacttc
    27661 gtcgtcgggc tcgccgagcg cacgcgtgat gcgccgcagg tcatcgccat cgacggtgtc
    27721 ggcgccttcg agcagctcgc cgtagaggcg cagcgccgcc gcgacttcga ccgcttcgtc
    27781 cgcatggccg gcgcttaccg gcttgtgaat gagcgagacc agcgctgtat cgggcgccgc
    27841 cttgctcgcg ccttgctcga cgcgatcgcg cacgagctcg agcaaatccg cgcgcatcag
    27901 cgacagctcc gcgctgcgct gcgcccgcaa ttcctcgacg acgcgcgcca acgtgccgaa
    27961 cgccgccgtg cgactcgcac catcgccgcg cccacgcctc aacgcacgcg ttagatgtcg
    28021 cgcggcttcg aggacccgct cgtgctggcc cgccgaatcc tgccgccgaa tctcggagac
    28081 gagctcatcg atggcgacgc ggccaatggg atccgattcg cgactcgcat ccgcgagctc
    28141 gccgatcagc gcgtcccagt cgcccgcgac gcgaagccgc gcgacctcgg ccgtcatcgc
    28201 gactgtcgcg gctccgcccg tcacgctatc tcgtcgcgcg cgcgagcctg gtcgggcgtc
    28261 tgcagtcacg gcgggtatcg ggtccgagac tcggtcgagc tcacctgatg aggctccagc
    28321 tgcctccgtg accgaggagc ggacgcgatc ggggtcgcgc cattgatcga atgcgtcggg
    28381 tggcacgccg ccggcgaggc cgagcgcgcg gatcgcgagc tcgcggagct tcggcttcgc
    28441 ggcttgggcc gcttcgagga gcgtcttgcc gtggacctcg gcgggtgcgt cgagcgtggc
    28501 cacgagcgcg gcccagacgg agggcatgcc ggtttcggtc gcagccttga cgagcggcgt
    28561 gacggcgtcg gtgcggcctt gatcgtggag gcggacgagg acttcggcgg cgcgttcggg
    28621 gcgcggaccg tcgagctctt tgatgagggc ttggacagcg cgatcgtcga tgacggcgcc
    28681 ttcttcgaga gcatcggtgc gcgagcgcgt ttcggcgacg tgctcgatcg cgagcgcggg
    28741 gagctctcgc gagatgcgga gggaggcgag cagccagagc gcggcggcga cggcggtgat
    28801 cacgtagaag acggtcggcg agctcgcgag cgcggcagcg atcaggccac cgacgacggc
    28861 gcctgcgcgc ttcgccgttc cctcgaggaa gccagcgacg cggccgcgca cgaccgtggg
    28921 gaggagcgtt tgggcgagct tttcggcggg cgtctcgatc gcggagtcga gcacctttgc
    28981 ggaggcctgc gtcgcgatcg cgacggcgag gatcggcgcg acgacgaggc cgaggcccga
    29041 ggccagcgcg acgaacggcg cgagcagcag cgcgcggccc gtgccgatgc gcgcgagcag
    29101 gccgcgtgcg acgaagagct ggacgacgag cgtgaggatt tgcgcgccac cgcgaacgcc
    29161 gccg
//
LOCUS       DATLBZ010000310         5644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10609327, whole genome shotgun sequence.
ACCESSION   DATLBZ010000310 DATLBZ010000000
VERSION     DATLBZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10609327"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VMZ53_15945"
     CDS             <1..260
                     /locus_tag="VMZ53_15945"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827241.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HUS29999.1"
                     /translation="GDGVRVDTHVEAGYVVPPFYDSLLAKLIVKAPTRDEAIAKMIGA
                     LSAFHVEGVPTTIPMHLAILKSDAFRTGEYDTRTIPGWPPA"
     gene            complement(264..779)
                     /locus_tag="VMZ53_15950"
     CDS             complement(264..779)
                     /locus_tag="VMZ53_15950"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HUS30000.1"
                     /translation="MTTTTLGLLKRTMPSPSFAPRLLLFLAAVGVSFVPSIVGKWFGP
                     DDWYASIEKSSLTPPGYVFPIVWTTLYLLMGIALWRFWESDASGSAKRAGTLLFGAQL
                     VLNGLWSYLFFGLHRPDLAMLDLALMWAAIAATIVTFGRYRRSAALLLVPYLVWVTFA
                     GYLNFAIWQMQ"
     gene            complement(776..1297)
                     /locus_tag="VMZ53_15955"
     CDS             complement(776..1297)
                     /locus_tag="VMZ53_15955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS30001.1"
                     /translation="MNVLTDKSSTSFGQNERAFVYAVARRIVQNDDDADDVAQDALLL
                     AHRNLASFRGDSRFRTWLYRIATTTALGHLRRVRRRKEHVPATEVALPDPAKSAEATV
                     GDAEMEALVRKHIERLDPLYRDVLIARADATESEVAVCLGISVANVKIRAHRARKQLR
                     DALEQELARGIAA"
     gene            1376..2218
                     /locus_tag="VMZ53_15960"
     CDS             1376..2218
                     /locus_tag="VMZ53_15960"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS30002.1"
                     /translation="MRHLSPRDLADALGVSESSLKRWVDSGKIVAFRTEGGHRRIALA
                     EAVRFVRETHAPLARPELLGLAEVAAAQRSMLTGSVRLFDFVSEGDVDGARGFLLARY
                     LAGATIAELADGPIREAMEELGEIWRHDSRGIFIEHRATDTCLQAIAHLRNMIDTPTN
                     APLAIGAAPEDDPYILPAFLATMVTTGAGLRTVNLGADTPLSALEQAVHHHRPRLVWM
                     SATAPINSARARSIQRYFDSLDSTTTVIVGGRHGRALAGRSRNVRYLDTLSELSSVAA
                     QLVA"
     gene            complement(2221..2907)
                     /locus_tag="VMZ53_15965"
     CDS             complement(2221..2907)
                     /locus_tag="VMZ53_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30003.1"
                     /translation="MQFRPTVIALLLVEVSCGRRAFELHDAARDGAPPPIDAITPDAV
                     SRAGCADDTREAFLDEMLFPEIAGCAATWNGTPSMRAPRTGSACGNGAVCAVPADACG
                     DGWHVCEDSGNPADLSTRVSGADCHTGGGVATGTFITASSHCSACANGCAAVEVDCVY
                     APPYGCAPTTSICSESVCCGPDCYQTHNCHGAVFAAPDTWINGAFSAPCGAMPGNMQT
                     GVLCCRDPAL"
     gene            2930..3475
                     /locus_tag="VMZ53_15970"
     CDS             2930..3475
                     /locus_tag="VMZ53_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS30004.1"
                     /translation="MSVRALRNQVISLAGSPPSLLCVTGASGVGKTTVLEALRREIEA
                     RHLPTLAFDSLGVPTPEQMEAGWESGRGWQKAMTWHWVQTAKNVYRTHPLVVLEGSFD
                     PQYAIAACTANRMRFSIVLLHKDLDSDWSKYLHESTQQLGGHVLDANAQTDAVLDAVC
                     GHAITLLGEAAASEAPKPRRN"
     gene            3498..5228
                     /locus_tag="VMZ53_15975"
     CDS             3498..5228
                     /locus_tag="VMZ53_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HUS30005.1"
                     /translation="MSFVPLVPIGSPLAGIVDDRTAADQRAALASLEDRLRARRAEVH
                     AGWGPTYVERVHKKGKLTSRERLARLADPGTRTFEVGTFVNYGEVFPGDQKSPAAGVV
                     TAFARVEGRWCMVIANDNTVASGAWWPRTPEKIQRAQMMALRLRIPTIYLVDCSGLFL
                     PEQSKSFPGAKGAGHIFKMNSLLSAGGVPQIAGVFGDCIAGGGYMPIISDRVYMTEQA
                     YMVIAGAALIKGAKSQKITSLDIGGPEVHVHQSGCADVRVPDDDTLLTCIRRDVARLP
                     SSGADFYRGDLGPVDPRFAPAELGAMVPADHREAYDANQVLARLTDQSLFWEVMPEVG
                     EEMICGIGRVGGLYAGFVINRQGLVGDPEHPGRQRPAGILYRGGIAKMSAFSRACNDD
                     GIPLIWLQDISGFDIGHEAEAHGLLAYGSSLIYTNSTNTTPMFTVLLRKASGAGYYAM
                     SGLPYDPIVQLSTSYARLSVMEGRTLAIAAYNTKLDDDFQIMATDPAERAKIEAGMRD
                     TEARIERDMDPFVAARQLDTDEIVELGELRAYLAALVEMAYQNTGYRRVKNPRIWSMH
                     DLRVLVGGPR"
     gene            5228..>5644
                     /locus_tag="VMZ53_15980"
     CDS             5228..>5644
                     /locus_tag="VMZ53_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30006.1"
                     /translation="MSALSTLEALVAGDEVRAPAPGWFHLLVAPDHLVAPGDVIGELE
                     VLGRIVRITAPRVHGLVKLEKRPSLARLPVSFGDVLFRVATDVAIAGTNALAGGTGAT
                     TRAEAGLVVRAPTSGRFYGRPSPDKPAFVEAGRELAA"
BASE COUNT          969 a         1920 c         1823 g          932 t
ORIGIN      
        1 cgggcgatgg cgttcgcgtc gacacgcacg tcgaagcggg ctacgtcgtg ccgccgttct
       61 acgactcact gctcgcaaag ctgatcgtca aagcgccgac gcgcgacgag gcgatcgcga
      121 agatgatcgg cgcgctctcc gcattccacg tcgagggtgt accgacgacg attccgatgc
      181 acctcgcgat tctgaagagc gacgccttcc gcacgggcga gtacgacacg cggacgattc
      241 cgggctggcc gccggcctaa cggtcactgc atctgccaga tcgcgaagtt cagatagccg
      301 gcgaatgtga cccacacgag gtacggcacg agcagcagcg cggcgctcct ccgatagcgg
      361 ccgaacgtga cgatcgtggc cgcgatcgcg gcccacatca gcgcgagatc cagcatcgcg
      421 aggtcagggc gatgcagacc gaaaaacaaa tacgaccaca gcccgttcag cacgagctgc
      481 gcgccgaaca ggagcgtgcc ggcgcgcttc gcggagcccg acgcgtcgct ctcccagaat
      541 cgccatagcg cgatgcccat gagcaggtag agcgtcgtcc acacgatcgg gaacacgtag
      601 cccggtggtg tcagcgatga cttctcgatc gacgcgtacc agtcgtcagg accaaaccac
      661 ttgccgacga tactcggcac aaagctcaca ccgaccgcgg cgaggaacag caggaggcgc
      721 ggcgcgaagc tcggcgacgg catcgtgcgc ttcagcaatc cgagcgtggt cgtcgtcatg
      781 cggcaattcc tcgtgcgagc tcttgctcga gtgcgtcgcg cagttgcttg cgagcgcggt
      841 gagctcggat cttcacgttt gcaacgctga tgccgagaca cacagcgacc tcgctttcgg
      901 tggcatccgc gcgcgcgatc agcacgtctc gatagagtgg gtcgagccgc tcgatatgtt
      961 tgcgcacgag cgcctccatc tcggcatcgc cgaccgtcgc ctccgcagac ttcgcaggat
     1021 ccggcagcgc cacctccgtt gccggaacgt gctctttgcg ccgacggacc cgccgcagat
     1081 gtccgagcgc cgtggtcgtc gcgatccgat acagccacgt gcgaaaccgc gagtcacctc
     1141 gaaagctcgc caggttgcga tgcgcgagga ggagcgcgtc ttgggcaacg tcgtctgcat
     1201 cgtcgtcgtt ctgaacgatg cggcgcgcga ccgcgtagac gaaggcgcgt tcgttctgac
     1261 cgaacgatgt ggacgactta tcggtcaaaa cgttcatctc gtccattgtg gatccagagg
     1321 gccgaatgtc aacggctctg taaccccgcg ccgttccggg gcatcctgtg ggtgaatgcg
     1381 acacctatcg ccgcgagacc tggcagacgc gctcggcgtc agcgagtcgt cgctgaagcg
     1441 atgggtcgac tccggcaaga tcgtcgcatt tcgtacggaa ggcgggcatc gccggatcgc
     1501 gctcgccgaa gccgtgcggt tcgtgcgcga gacccacgcg ccgctcgccc gccccgagct
     1561 gctcggcctt gccgaggtcg cggctgccca gcgctcgatg ctcaccggaa gcgtgcggct
     1621 gttcgacttc gtcagcgaag gcgacgtcga cggcgcgcgt ggctttctgc tcgcgcgtta
     1681 cctcgccggt gcgacgatcg cggagctcgc cgacgggccg attcgcgaag cgatggaaga
     1741 gctcggtgag atatggcggc acgattcgcg cggcatcttc atcgagcacc gcgcgacgga
     1801 cacctgcctg caggccatcg cgcacttgcg caacatgatc gatacgccga cgaatgcgcc
     1861 actcgccatc ggtgcagcgc ccgaggacga tccgtacatc ttgcccgcgt tcctcgcgac
     1921 gatggtcacg acaggcgcag gcctacgcac cgtcaatctc ggggctgaca cgccgctctc
     1981 cgccctcgag caggcggtgc atcatcatcg cccgcgcctt gtctggatga gcgccacggc
     2041 gccgatcaac tccgctcgtg cccggtcgat ccagcgctac ttcgattcgc tcgactcgac
     2101 gacgacggtg atcgtcggtg gcagacatgg acgcgcgctc gccggcaggt cgcgcaacgt
     2161 tcgctacctc gacacgctca gcgagctcag ctccgtcgca gcgcaactcg tcgcatagag
     2221 ctacagcgcc gggtcgcgac agcagagcac acccgtctgc atgttgcctg gcatcgcgcc
     2281 gcacggcgca ctgaatgctc cgttgatcca agtgtcgggt gcagcgaaca cggcgccgtg
     2341 acagttgtgc gtctgatagc agtcagggcc acagcaaacg ctctccgagc agatcgacgt
     2401 cgtcggtgcg cagccatacg gcggcgcgta gacgcaatcg acctcgacag cggcacatcc
     2461 gtttgcacac gctgaacagt ggctcgacgc cgtgatgaac gttccggtcg cgacgccgcc
     2521 gcccgtgtga cagtctgcgc cggacacgcg cgtgctcagg tccgctgggt tgccgctgtc
     2581 ctcgcatacg tgccagccat cgccacacgc atctgccggt accgcgcaca ccgcgccgtt
     2641 gccgcacgca gagccggttc gaggggcgcg catgctcggc gtgccgttcc acgtcgccgc
     2701 gcagccagcg atctcgggga acagcatctc gtcgaggaac gcctcgcgcg tgtcgtcggc
     2761 gcagccggcg cgtgagacgg cgtcgggcgt gatcgcatcg atcggaggcg gcgcgccgtc
     2821 gcgagcggca tcgtgcagtt cgaacgcgcg tcgcccgcag ctaacctcga cgaggaggag
     2881 cgcgatcacc gtcggccgaa actgcatttc gtagtagcgt acgcgctgcg tgtcggttcg
     2941 tgcacttcgc aaccaggtca tctcgctcgc aggatcgccg ccgtcgctgc tgtgcgtgac
     3001 aggcgcatct ggtgtcggta agacgacggt gctcgaagcg ctgcggcgtg agatcgaagc
     3061 gcgccacctg ccgacgcttg cgttcgacag cctcggcgtt ccgacgcccg aacaaatgga
     3121 agcgggttgg gagtcgggcc gcggctggca gaaggcgatg acgtggcact gggtccagac
     3181 cgcgaagaac gtctaccgaa cgcatccgct tgtcgtgctg gagggctcgt tcgatccgca
     3241 atacgcgatc gctgcgtgca ccgcgaaccg catgcggttt tcgatcgtcc tccttcacaa
     3301 agacctggat tcggattggt ccaagtacct gcacgaatcc acacagcagc tgggcggtca
     3361 cgtcctcgac gcaaacgccc agaccgatgc cgtcctcgac gcggtgtgcg gacacgcgat
     3421 cacgctgctc ggtgaggccg ccgcgagcga ggcgccgaag ccccgccgca attgatgatg
     3481 tagtacgttg cgcccgcatg tcgttcgtac cgctggttcc gattggaagt ccgctcgccg
     3541 gaatcgtcga cgaccgcacc gccgctgacc aacgcgccgc gctcgcatcg ctcgaagatc
     3601 ggttgcgtgc tcgtcgcgcg gaagtgcatg caggctgggg cccgacgtac gtcgagcgcg
     3661 tgcacaagaa aggcaagctc acgagccgag agcgccttgc acggctcgct gatcccggca
     3721 cgcgcacgtt cgaagtcggc acgtttgtga actacggcga ggtgttccca ggcgatcaga
     3781 agtcgccggc cgctggcgtc gtcaccgcgt tcgcgcgcgt cgaaggacgc tggtgcatgg
     3841 tgatcgcgaa cgacaacacc gtcgcgtcgg gcgcatggtg gccacgcacg cccgagaaga
     3901 tccagcgcgc ccagatgatg gcgctgcgcc tgcgcatccc gacgatctat ctcgtcgatt
     3961 gctcgggcct ctttctcccg gagcaatcga agtcattccc gggcgcgaag ggcgcaggcc
     4021 acatcttcaa gatgaactcg ctgctgtccg ccggcggcgt gccccaaatc gcgggcgtgt
     4081 tcggcgactg catcgcgggc ggcggctaca tgccgatcat ctccgaccgc gtctacatga
     4141 ccgagcaggc gtacatggtg atcgcgggcg ctgcgctcat caagggcgcg aagagccaga
     4201 agatcacgtc gctcgacatc ggtggccccg aagtgcacgt ccaccaatcg ggttgcgctg
     4261 atgtccgggt tccggacgat gacacactgc tgacgtgcat ccgtcgcgac gttgcccgcc
     4321 tgccctcgag cggcgcggac ttctatcgcg gtgacctcgg tcccgtcgat ccgcggttcg
     4381 cgcccgccga gcttggtgcg atggtgccgg ccgatcatcg cgaggcgtac gacgccaacc
     4441 aggtcctcgc gcgcctcacc gatcaatcgc tgttctggga agtgatgccg gaagtcggcg
     4501 aggagatgat ctgcggcatc ggtcgcgtcg gcggcctcta cgccggcttc gtgatcaacc
     4561 gccaaggtct cgtcggcgat cccgagcatc ccggccgcca gcgtcccgcg ggcatcctct
     4621 accgcggcgg catcgcgaag atgagcgcgt tctctcgcgc gtgcaacgac gacggcatcc
     4681 cgctcatctg gctccaagac atctccggct tcgacatcgg ccacgaagcc gaggcgcacg
     4741 gcctgctcgc gtatggcagc tcgctgatct acacgaactc gacgaacacg acgccgatgt
     4801 tcacggtgct gctccgcaaa gcttcaggcg ccggctacta cgcgatgagc ggcctgccct
     4861 acgatccgat cgtccaactc tcgacgagct acgcgcggct ctccgtcatg gaaggccgca
     4921 cgctcgcgat cgctgcgtac aacacgaagc tcgacgacga cttccagatc atggcgacgg
     4981 atccggcgga gcgcgcaaag atcgaagcag gcatgcgcga caccgaagcg cgcatcgagc
     5041 gcgacatgga tccgttcgtc gccgcgcgcc agctcgacac cgacgagatc gtcgagctcg
     5101 gcgagctccg cgcatacctg gccgcgctcg tcgagatggc gtaccagaat accggctatc
     5161 gccgcgtgaa gaacccgcgc atctggtcga tgcacgatct tcgcgtgctc gttggaggcc
     5221 cgcgctgatg tccgcgctgt cgacgctcga ggccctggtc gccggcgacg aagttcgcgc
     5281 gcccgcaccg ggctggttcc acctgctcgt cgcgcctgat catctcgtcg cgcccggtga
     5341 cgtgatcggc gagctcgaag tgctcggccg catcgtccgc atcaccgcgc cgcgggtgca
     5401 cggtctcgtg aagctcgaga agcgaccgtc gcttgcgcgc ctacccgtca gcttcggtga
     5461 cgtgctgttc cgcgtggcga cggatgtcgc gatcgcaggc acgaacgcat tggctggcgg
     5521 caccggcgcg actacgcggg cagaggccgg cctcgtagtc cgcgcgccca cgagtggtcg
     5581 cttctacggt cggccatcgc ccgacaagcc cgcgttcgtc gaagcagggc gcgagctcgc
     5641 cgcc
//
LOCUS       DATLBZ010000311        12092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4057077, whole genome shotgun sequence.
ACCESSION   DATLBZ010000311 DATLBZ010000000
VERSION     DATLBZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12092
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4057077"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VMZ53_15985"
     CDS             complement(<1..794)
                     /locus_tag="VMZ53_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30007.1"
                     /translation="MISPELELHDLDARHWRNGWRLLAPPRVLDRPRWALVVVDGSPD
                     RVLKVIVAGDRARGSIDPASAPLAGVTEKALAAYARTLDVGAVIVVDRHLIRDLSSEI
                     EAALRFDMDGMAQSLVALRALKKHAGKGIWTEPPLLDLLPAPHAEPIQRTFDLLIPNN
                     TALLAYVIEDDRSRVHTSIIATKQEGDITRVAQHRAIADLVSEPTLARDWQTGYKRVL
                     EAVEERFAKPSVAVFLERATLMKILTGPSDQLAREINAKNVIIDPAP"
     gene            844..1377
                     /locus_tag="VMZ53_15990"
     CDS             844..1377
                     /locus_tag="VMZ53_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30008.1"
                     /translation="MMKTRLFGALLVVVASAGVANAGGQAGSIGAGVEAQINGAGGAS
                     MTYDAGDFHVGGFLGFHDPGGSNDDVILIGGRFFYHLHSTAMSDFGLGGTLGIVSQNN
                     DPPVDRQTYLFIEPSFQIRAFVTSNVALSFNAGVVIGVMDADNVDFGGQLVGGGVSVA
                     GAGVNVTGGAGIHYYFF"
     gene            complement(1381..2382)
                     /locus_tag="VMZ53_15995"
     CDS             complement(1381..2382)
                     /locus_tag="VMZ53_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30009.1"
                     /translation="MAKAKTAAPKKAAAPRKPKKVKAAAGSVGLTAEECADGSPPAEV
                     TKLKKEIENAGGQALALYREPLGGNWVALAALPLERVKPTPYQRELSKTHADRLAAVI
                     PKVGRFLDPVIAVPDGKGDFISPNGMHRLTAMGTLGAKTIVALVMPEPEIAFRILALN
                     TEKAHNLKDKALEVVRMARAIAEDDDRSEQAMAFELEQPALVTIGVCYEKNPRFSGGA
                     YNSVVTKCESFSTEKMSKSLKLREEHADKLLELDKLVGEAVQKLKDAGWTSGYLKPIV
                     VARINPLRFVKAGADEKSDFDKTIDKMIEGAKKFDASKVKAQDVVLAATVGGGEDLN"
     gene            2417..3643
                     /gene="serA"
                     /locus_tag="VMZ53_16000"
     CDS             2417..3643
                     /gene="serA"
                     /locus_tag="VMZ53_16000"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HUS30010.1"
                     /translation="MAETSFPKSEIKILLLENVHPVARDAFAAEGFQVEMVKSALPED
                     ELVERVRDAHVLGIRSKTQVTPRVLDEAKRLLTVGAFCIGTNQIDLLHANRKGTPVFN
                     APFSNTRSVAELIIAEIVMLSRHLGDRVREVHQGQWKKVAVGSHEVRGKTIGIVGYGH
                     IGSQVGVLAEGFGMRVMFYDIAAKLPMGNNRACSTLEEVLAESDFVTLHVPETPQTKD
                     MIGHAELAQMKKGACLLNASRGTVVVIESLAAALQSGHIGGAAVDVYPREPESNSDGF
                     DTPLRGLQNVILTPHIGGSTEEAQEAIGKEVSAALIKFINTGGTTGSVNFPQVELPIA
                     KGAHRILNVHRNVPGVLRDINRIVSDMNANIRAQLLSTDPDIGYLIMDMDQDVSQDVK
                     KAIANLPTSIKTRILF"
     gene            3661..5040
                     /locus_tag="VMZ53_16005"
     CDS             3661..5040
                     /locus_tag="VMZ53_16005"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HUS30011.1"
                     /translation="MHISVFDIFKIGIGPSSSHTVGPMRAARRFAERLEADGQFAQVA
                     GLKVELFGSLGFTGKGHGTDKAVILGLEGEDPATVDVDSVARRVAAVEQSHRVKLLGA
                     HEVELDPATSLIFHRREKLPLHANGMRFTALGAGGATVVDRIYYSVGGGFVVDHAGVP
                     ADGATPADQVQVPYPFNSGAELLRHAAEHGMAISSLMLENEKTLCSEADVRRRVMEIS
                     AAMDACIKRGTEREGILPGGLKVKRRAAALFRKLKSDPRGGNDPLVIMDWVNLFALAV
                     NEENAAGGRVVTAPTNGAAGIIPSVMTYYRRFLPNSDDEGSVRFLLTAAAIGALYKKN
                     ASISGAEVGCQGEVGVACSMAAGALAEVMGGTPAQVENAAEIGMEHNLGLTCDPIGGL
                     VQVPCIERNAMGAVKAINAARLALQGDGTHKVSLDKVIKTMWRTGADMSSKYKETARG
                     GLAVNIIEC"
     gene            5087..7006
                     /locus_tag="VMZ53_16010"
     CDS             5087..7006
                     /locus_tag="VMZ53_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30012.1"
                     /translation="MEILGLEARRSDLARGILIARSTRLLDAAEQKALWDALAAAGVT
                     EQADEDTEDEPSRQWHAAPWLPPDLAQVDPQRVAIEATAMPFAIDCVWLVKIETSIDG
                     VAWLEMTAAEAPTEEEDDSGEPGGAEGEEAGGVLDENREVIYAAPDAPDAPEDDEADK
                     EDDEPDDEEESIEELDVVALESHWRNGAPPMEHSVPFPIERYPEIIDDYDWESFGIAF
                     KLSGTAAAGEESVVNAFFALWLSVYQDERAEEGTGPQSYEPFQRADVIHDRKHRSALM
                     WVERFSVPATASDQVHFLLWVMARLHDVLPLAWARFDTVDDSVKAAAAGDYDEIPFVL
                     AGNPFADRFQRQGEEAALAWAVSQSLWSKRELAGMLVEVALEHDPDNTETATIAERLL
                     RRALSFEPKSEAAGYLCIVLVRQNRIAEAITIATNAASRDVRLLVIGEVAEHAGDELP
                     PALDLLDAETLSETSPEELAELTASVARHASQHLAVLLRKLPNDVALVPHLYNASFSV
                     DRPLALAILRRVLTLPEPPRETGEARTALVMAWNNACIHAHALGDYKLAVELADGGQP
                     FGPENPYIYHSAACAYAAVGEIERALAQVRLAIEHEYEHTEKMETDADLSPLHADPRF
                     PALFNEWRERRADLN"
     gene            complement(6999..7610)
                     /locus_tag="VMZ53_16015"
     CDS             complement(6999..7610)
                     /locus_tag="VMZ53_16015"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003300.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HUS30013.1"
                     /translation="MAKLYFRYGTMDSAKSMNLLAVAHNYRKQGKKVLLVKPRLDTRF
                     GATKISSRCGLEADADLMVDDEMSLDPREFAGLDCVLVDEAQFLAPSVIEDLRRITVD
                     PGVPVICYGLRTDFQTKLFPGAQRLMELADRIEEVKVTCQYCAKKAICNLRFVNGAPT
                     VEGPQIQLGADEHYAPVCWGHYDEATRHISVANEKSGAVLRPS"
     gene            complement(7665..8672)
                     /locus_tag="VMZ53_16020"
     CDS             complement(7665..8672)
                     /locus_tag="VMZ53_16020"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HUS30014.1"
                     /translation="MDVTTTMYVTIAVALLFDIINGFHDAANSIATVVSTRVLSPRVA
                     VLWATIFNFVALFVFHQGVANTIAKIITIEGSDHAFVWVVACGLVGAIVWNLLTWWWG
                     LPSSSSHALIGGLSGAGIAYKGTHVLNTTKLYPTLQFIILAPLIGLVLGALLMFLVYW
                     IFRRMRPRRVDSVFRVGQLFSAAAYSIGHGGNDAQKTIGVIWAVMIAGGWKGAEHGVP
                     YWVILASYSAMAFGTALGGQRIIKTMGMGITSLKPAGGFSANLAGAITLFGATGLHIP
                     VSTTHTITGAIVGVGSVTKLRGIRWGLATRIVWAWIFTIPLAGLVGAGSLYIARALNA
                     W"
     gene            complement(8672..9298)
                     /locus_tag="VMZ53_16025"
     CDS             complement(8672..9298)
                     /locus_tag="VMZ53_16025"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HUS30015.1"
                     /translation="MFGRFLPRETSFFDFFEQHAGLTIEGTKEFLSMVTTGANIPAKC
                     RRISDIEHETDTITHRCVEALHKTFITPIDRDSIHRLITRMDDIMDYVEAAAERIELY
                     ELTTMTADVRDLADVLHRSSLQVEQAVRGLRQLKTPQATLKLCIDINRLENEADAILR
                     RSVARLFKDEKDPILVIKWKEVYENLEMASDRCEDVANIIEGTILEHT"
     gene            complement(9327..9974)
                     /locus_tag="VMZ53_16030"
     CDS             complement(9327..9974)
                     /locus_tag="VMZ53_16030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS30016.1"
                     /translation="MKPLVVLLHGLARGHGSMAKLSTHLRKHGFDTWSTTYPSRQHSI
                     AYLASALSEQIVDAAGDRPVSAVTHSMGGVIVRHLQDPRLRWDRIVMLAPPNRGSRLA
                     AGLVRNPLFQFFYGPAGLELADATAWPLPPVPPAVIAGTRSLALTNVTSWTVGRRFPV
                     GVKNDGTVAVEETKLDQMAAFAEVDATHTWIMNSDAVRHLVVTYLSEGCFPRVTP"
     gene            complement(9971..11428)
                     /locus_tag="VMZ53_16035"
     CDS             complement(9971..11428)
                     /locus_tag="VMZ53_16035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUS30017.1"
                     /translation="MAYRILAWFLRVVTRVFFRQIEVVGLEHVPKDGPVLFAGNHPNS
                     LIDPILIITTCGRKVHFAAKDALFKGRIMRALLNGLGAVPIKRRDDHDGSRESKPDVA
                     TKAPVDNDAAFTAMFQVLGDGGAIGIFPEGLSHDESQLARLKTGAARLALGGAQRTSQ
                     PIHIVPCGLTFIHPKRFRSRVLVQFGPAITVENARPNTPDEVRELTSEIETGMRRLTI
                     NAPDWDTVRALDVVRRLYQPQEISIEDRVELARRFNAYYGAVAADPRVVALMQRVRAY
                     QQKLDELGLSDRELARDLSKLEVSARVLRHLVLVAFWLPLTTPGAALHVPPLVFARLA
                     GPRLTPRKDVVATTKLLIGLMLVLLAYAAAIGVLWWKAGLWWAVAAMIVLPLSGIATL
                     KVLDRLRLVRRGLGVLFRRLRFRKEVQALRNEREQLADDVVATVNAVKPAELESLFPA
                     DHPDRLEESWRSRRDADLDAELDKDAAEARADGES"
     gene            complement(11445..>12092)
                     /locus_tag="VMZ53_16040"
     CDS             complement(11445..>12092)
                     /locus_tag="VMZ53_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30018.1"
                     /translation="AFEIADAMKDAEVRRILRNPFALDPDPDGPHPKGDERLVGWDSY
                     LKMFFVPFFMAATNAPVVRRGHALAGHPWGEDFVYREVMSTPGNPRGAVMAAGITAGL
                     AGLSAAMKRPGLRSMLKKRAPQPGEGPSPEKRERGHWKVRFVAEDGDDKLLYIVADPH
                     GDPGYKSTAKMLGESALCLAYDELESAGGVQTPSVAMAGALLERLRTAGLIFQPR"
BASE COUNT         2146 a         3851 c         3989 g         2106 t
ORIGIN      
        1 ggcgccggat cgatgatgac gttcttcgcg ttgatctcgc gggcgagctg atcgctcggg
       61 ccggtgagga tcttcatcag cgttgcgcgc tcgaggaaca cagcgaccga cggcttggcg
      121 aatcgctcct cgaccgcttc gagcacgcgc ttgtagccgg tctgccagtc ccgcgcgagc
      181 gtgggttcgc tgacgaggtc ggcaatggct cgatgttggg ccacgcgtgt gatgtcgcct
      241 tcttgcttcg tcgcgatgat cgatgtgtgg acacgcgagc gatcgtcttc gatgacgtac
      301 gcgagcagcg cggtgttatt gggaatcagt aggtcgaacg tgcgctggat cggttcggcg
      361 tgcggcgcgg ggaggaggtc gaggagcggc ggctctgtcc agatgccttt gcccgcgtgc
      421 ttcttgagcg cgcgcagcgc gaccagcgat tgcgccatgc catccatgtc aaagcgcagc
      481 gccgcctcga tctccgacga gagatcgcgg atgagatggc ggtcgacgac gatgactgcg
      541 ccgacatcga gcgtgcgcgc gtatgcggcg agcgccttct cggtcacgcc ggccagcggt
      601 gccgacgctg gatcgatgga gccgcgcgcg cgatcgcctg caacgatcac tttgaggacg
      661 cggtcaggtg agccatcgac gacgacgaga gcccagcgag gccgatcgag cacgcgcggc
      721 ggcgcgagca gacgccatcc gttgcgccag tgacgcgcat cgagatcgtg cagctcgagc
      781 tcgggcgaga tcacgccggt agcatacttg attgtcggat cggctcgggt catcgtccgg
      841 ggcatgatga agacccgctt gtttggtgca ctgttggtgg tggtggcgtc ggccggcgtg
      901 gcgaatgccg gaggtcaggc tggctcgatc ggcgctggtg tcgaggcgca gatcaacggc
      961 gccggtggcg catcgatgac gtacgacgcc ggcgattttc atgtcggcgg cttcctcggg
     1021 tttcacgatc cgggtggcag caacgacgac gtgatcctga tcggcggccg gttcttctat
     1081 cacctgcact cgaccgcgat gagcgacttc ggcctcggtg gcacgctcgg catcgtcagc
     1141 cagaacaacg atccgccggt cgatcgccag acgtacctgt tcatcgagcc gagctttcag
     1201 atccgcgcgt tcgtcacgtc gaacgtcgcg ctgagcttca acgccggcgt cgtgatcggc
     1261 gtgatggatg ccgacaacgt cgacttcggc ggtcagctcg tcggcggcgg cgtgtccgtc
     1321 gcgggtgcgg gcgtcaacgt cacgggcggc gctggcatcc actactactt cttctagaga
     1381 ctagttcagg tcttcgccgc caccgaccgt tgcggcgagc acgacgtcct gcgccttgac
     1441 cttcgacgcg tcgaacttct tggcgccttc gatcatcttg tcgatggtct tgtcgaagtc
     1501 ggacttctcg tcggcgcccg ctttgacgaa gcgcaacggg ttgatgcgcg cgacgacgat
     1561 gggcttcaga tagccgctcg tccagccggc gtctttgagc ttctgcacgg cttcgccgac
     1621 gagcttgtcg agctcgagca gcttgtccgc atgttcctcg cggagcttca gcgacttcga
     1681 catcttctcc gtcgagaacg actcgcactt cgtgacgacc gagttgtacg cgccgcccga
     1741 gaagcgcgga tttttctcgt agcagacacc gatcgtgacg agcgcgggct gctcgagctc
     1801 gaacgccatc gcctgctcgc tgcgatcgtc gtcctcggca atcgcgcgcg ccatgcgaac
     1861 gacctcgagc gccttgtcct tcaggttgtg cgctttctcg gtgttgagcg cgaggatgcg
     1921 gaatgcgatt tcaggctccg gcatcacgag cgctacaatg gtctttgcgc cgagcgttcc
     1981 catcgcggtg aggcggtgca tgccgtttgg cgagatgaag tcgcccttgc cgtcggggac
     2041 cgcgatcacc ggatcgagga agcggccgac tttcggaatc accgccgcaa gtcgatcggc
     2101 gtgcgtcttc gacagctcgc gctggtatgg agtgggcttc actctctcga gcgggagtgc
     2161 cgcgagcgcg acccagtttc caccgagcgg ttcgcgatag agggcgagcg cttggccacc
     2221 cgcgttctcg atctccttct tcagcttcgt cacttcggcg ggaggtgagc catccgcgca
     2281 ctcctcggcg gtcagaccga cggagccggc agcggccttc accttcttgg gcttgcgtgg
     2341 cgcagctgct ttcttgggcg ctgctgtctt ggcttttgcc atggccggac tctactcctg
     2401 gtacgttatc cgtcgcatgg cagagacgtc gtttccgaag tccgagatca agatcctgct
     2461 actcgagaac gtgcatccgg tagcgcgaga cgcattcgcc gcggagggct tccaggtcga
     2521 gatggtgaag agcgcgctcc ccgaggacga gcttgtcgag cgcgttcgtg acgctcacgt
     2581 cctcgggatt cgcagcaaga cgcaagtgac gccgcgcgtg ctcgacgaag cgaagcgcct
     2641 gctcaccgtc ggtgcgttct gcatcggtac gaaccagatc gatctcctgc acgcgaatcg
     2701 caagggcacg ccggtcttca acgcgccatt ctcgaacacg cgttcggtcg ccgagctgat
     2761 catcgcggag atcgtgatgc tgtcgcgtca cctcggcgat cgcgtacgcg aagtgcatca
     2821 aggacagtgg aagaaggtcg ccgtcggcag ccacgaggtg cgcggcaaga cgatcggcat
     2881 cgtgggctac ggccacattg gcagccaggt cggcgtgctc gccgaaggct tcggcatgcg
     2941 cgtgatgttc tacgacatcg cggcgaagct accgatgggc aacaaccgcg cgtgcagcac
     3001 gctcgaagaa gtgctcgccg agtccgactt cgtcacgctg cacgttccag agacgccgca
     3061 gacgaaggac atgatcggtc acgccgagct cgcgcagatg aagaagggtg cgtgcctgct
     3121 caacgcgagc cgcggcaccg tcgtcgtgat cgaatcgctc gctgccgcgc tgcagagcgg
     3181 ccatatcggc ggcgccgccg tcgacgtcta tccgcgtgag cccgagtcca acagcgatgg
     3241 cttcgatacg ccgctgcgcg gccttcagaa cgtgatcctc acgccgcaca ttggtggctc
     3301 cacggaagaa gcgcaggagg cgatcggcaa ggaggtctcc gcggcgctca tcaagttcat
     3361 caacaccggc ggcaccaccg gcagcgtgaa tttcccgcag gtcgagttac cgatcgcgaa
     3421 gggcgcgcac cgcattctca acgttcaccg gaacgtcccc ggcgtgctcc gcgacatcaa
     3481 ccgcatcgtc tcggacatga atgccaacat tcgcgcgcag ttgctgtcga cggatccgga
     3541 catcggctac ctcatcatgg acatggatca ggacgtctcg caggacgtga agaaggccat
     3601 cgcgaacctg cctacgagca tcaagacgcg catccttttc taggtcacac ttagtattcg
     3661 gtgcacatct ctgtcttcga catcttcaag atcggcattg gtccgtcgag ctcgcacacc
     3721 gtcgggccta tgcgcgcagc gcgccggttc gccgagaggc tcgaggccga tggccagttc
     3781 gcgcaggtcg ccgggctgaa ggtcgagctg ttcggaagcc ttggcttcac cggcaagggc
     3841 cacggcaccg acaaggcggt catcctaggg ctcgagggtg aagatcccgc gacggtcgat
     3901 gttgattcgg tcgcgcgtcg cgtcgccgcc gtcgagcaga gccatcgcgt caaactgctc
     3961 ggcgcccacg aggtcgagct cgatccggcg acctcgctca tctttcatcg tcgcgagaag
     4021 cttccgctgc acgcgaacgg catgcgcttc accgcgctcg gcgccggcgg cgcaacagtc
     4081 gtcgatcgca tctactactc ggtgggcggt ggctttgtcg tcgatcatgc cggtgtgccc
     4141 gcggatgggg cgacgccggc ggaccaagtc caagttccgt atccgttcaa ctccggcgcc
     4201 gagctcctgc gccacgcggc cgagcacggg atggcgatca gctcgctgat gctcgagaac
     4261 gagaagacgc tctgtagcga agccgacgtt cgccgtcgcg tgatggaaat ctccgccgcg
     4321 atggacgcct gcatcaagcg cggcaccgag cgtgaaggca tcttgcccgg cggcctcaag
     4381 gtgaagcggc gagctgcggc gctgtttcgc aagctcaaga gcgatccgcg tggcggcaac
     4441 gacccgctcg tcatcatgga ctgggtgaac ctgttcgcgc ttgcggtcaa tgaagagaac
     4501 gcagctggcg gtcgcgtcgt cactgctccg acaaacggcg cggcgggcat catcccgtcg
     4561 gtgatgacgt actaccggcg ttttctgccg aactccgacg acgaaggctc ggtgcgcttc
     4621 ctgctcacgg cggctgcaat cggcgcgctc tacaagaaga acgcctcgat cagcggtgcg
     4681 gaggtcggct gccaaggcga agtcggcgtc gcgtgctcga tggccgcggg cgcactcgcc
     4741 gaggtcatgg gcggaacgcc cgcgcaggtc gagaacgccg ccgagatcgg gatggagcac
     4801 aacctcggtc tcacgtgcga tccgatcggc ggcctcgtgc aggtgccgtg tatcgagcgc
     4861 aatgcgatgg gcgcggtgaa agcgatcaac gccgcgcgac tcgcactgca aggcgacggc
     4921 acgcacaagg tgtcgctcga caaagtcatc aagacgatgt ggcgaaccgg cgccgacatg
     4981 agcagcaagt acaaagagac tgctcgcggt ggactcgccg tgaacatcat cgagtgctga
     5041 gcgccacgcg atcattcgcg cgtggaagcc tgatacgctg ctcgaagtgg agatcctcgg
     5101 actcgaggct cggcgcagcg acctggcacg cggcattctg atcgcgcgca gcacacgctt
     5161 gctcgacgca gcagagcaaa aggcgctctg ggacgcgctc gccgcggcgg gcgtgaccga
     5221 gcaagccgac gaggacaccg aggacgagcc ttcgcgccag tggcacgccg caccgtggct
     5281 gccgcctgat ctcgcgcagg tcgatccaca acgcgtcgcg atcgaggcga cggcaatgcc
     5341 cttcgcgatc gactgcgtgt ggctcgtgaa gatcgagacg tcgatcgacg gcgtcgcatg
     5401 gctcgagatg acggccgccg aggctccgac cgaggaagaa gacgatagcg gggagccggg
     5461 cggtgcagag ggcgaagaag cgggcggcgt cctcgacgag aatcgggaag tcatctacgc
     5521 cgcgcccgat gcccccgacg cgccggagga cgacgaggcc gacaaggaag atgacgagcc
     5581 agacgacgaa gaggagtcga tcgaggagct cgacgtcgtc gcgctcgaga gccactggcg
     5641 caatggcgct ccgcccatgg agcacagcgt gccgttcccg atcgagcgct atcccgagat
     5701 catcgacgac tacgattggg agagcttcgg catcgcgttc aagctatccg gcaccgcggc
     5761 ggcgggagaa gagtcggtcg tcaacgcgtt cttcgcgctg tggctctcgg tctaccaaga
     5821 cgagcgcgcg gaagagggca ccggtccgca gagctacgag ccgtttcagc gcgccgacgt
     5881 gatccacgat cgcaaacatc gctccgcgct gatgtgggtc gagcgattca gcgtaccggc
     5941 gacagcgtcg gatcaagtgc acttcctctt gtgggtgatg gctcggcttc acgacgtgct
     6001 accgctcgcc tgggcgcggt tcgataccgt cgacgattcg gtgaaggcgg ccgccgctgg
     6061 tgactacgac gagattccgt tcgtgctcgc gggcaacccg ttcgccgatc ggtttcagag
     6121 gcagggcgag gaagccgcgc tcgcatgggc ggtgtcacag agcttgtgga gcaagcgcga
     6181 gctcgcgggg atgctcgtcg aggttgcgct cgagcacgat cccgacaaca ccgagaccgc
     6241 gaccatcgcc gagcgcctgt tgcgccgcgc cctgtcgttc gaacccaagt cggaagcggc
     6301 cggttatctg tgcatcgtgc tcgttcgcca gaaccgcatc gccgaggcca tcacgatcgc
     6361 gaccaatgcg gccagccgag atgttcgcct gctcgtgatc ggcgaggtcg cggagcacgc
     6421 gggcgacgag ctaccacccg cgctcgacct gctcgatgcc gagacgctca gcgagacctc
     6481 tcccgaggag ctggccgagc tcacggcgtc ggtcgcgcgt cacgcgtcgc aacacctcgc
     6541 tgtgctgctc cggaagctgc cgaacgacgt cgcgctcgtc ccgcatctct acaatgcgag
     6601 cttctccgtc gatcgtccgc tcgcgctcgc gattcttcgc cgcgtgctca cgctgccgga
     6661 gcctccgcgc gagaccggcg aggcgcgcac ggccctcgtg atggcgtgga acaacgcttg
     6721 tattcacgcg catgcgctcg gcgactacaa gctcgcggtc gagctcgccg acggtggcca
     6781 gcccttcggt ccggagaacc cgtacatcta tcactccgcc gcatgcgcct atgccgcggt
     6841 cggcgagatc gaacgcgcac tcgcgcaagt acgcctcgcg atcgagcacg agtacgagca
     6901 caccgagaag atggagaccg acgcggacct ctcaccactg cacgcggacc cgcgattccc
     6961 agcgctgttc aacgaatggc gcgagcggcg cgcggatctc aactaggacg cagcacggcg
     7021 ccagattttt cgttggccac tgaaatgtgg cgcgtcgctt cgtcgtaatg accccagcac
     7081 accggtgcgt agtgctcgtc ggcgccgagc tggatctgag gaccttcgac cgtcggcgcg
     7141 ccattgacga accgcaggtt gcagatcgct ttcttcgcgc agtactggca ggtgaccttc
     7201 acctcttcga tgcgatcggc cagctccatc aagcgttgcg cgcccgggaa tagcttcgtc
     7261 tggaaatcgg tgcgcaggcc gtagcagatc acgggcacgc cgggatcgac ggtgatgcgt
     7321 cgcaggtctt cgatcacgct cggcgccaag aactgcgcct catcgacgag cacgcaatcg
     7381 aggccggcaa actcgcgcgg atcgagcgac atctcgtcgt cgaccatcag gtccgcgtcg
     7441 gcttcgaggc cgcaccgcga cgagatcttc gtggcgccaa agcgcgtgtc gagacgcggc
     7501 ttgacgagca ggaccttctt gccctgtttg cggtagttgt gcgccaccgc caagaggttc
     7561 atcgacttcg ccgaatccat cgtgccgtat cggaagtaga gcttcgccac gtcggcgacc
     7621 ctagatcgcc cccctgacaa ggcccccgag tcgagcggga gcggctacca ggcgttcagc
     7681 gcgcgggcga tgtacaggct gccggcaccg acgagccccg ccaatggaat cgtgaagatc
     7741 cacgcccata cgatccgcgt agcgaggccc cagcggatgc cgcggagctt cgtcaccgag
     7801 ccgacgccga caatcgcgcc tgtgatcgtg tgcgtcgtcg agaccggaat gtgcagaccg
     7861 gttgcaccga acagcgtgat ggcgcccgcg aggttcgcgc tgaaaccacc cgcgggcttt
     7921 agtgacgtga tgcccatgcc catcgtcttg atgatgcgct ggccgccgag tgccgtgccg
     7981 aacgccatcg cactgtacga cgcaaggatc acccaatagg gcacgccatg ctcagcgccc
     8041 ttccagccac cagcgatcat cacggcccag atgacgccga tcgtcttctg cgcgtcgttg
     8101 ccgccgtgtc cgatcgaata ggcggcggcc gagaatagct ggccaacgcg aaacacgctg
     8161 tcgacgcggc gcggtcgcat ccggcggaag atccagtaca cgaggaacat caacagcgcg
     8221 ccgaggacca gaccgatcag tggggcgagg atgatgaact gcagcgttgg atagagcttt
     8281 gtcgtgttga gaacgtgggt gcctttgtag gcgatgccgg cgcccgagag cccaccgatg
     8341 agagcgtgcg agctcgacga aggaaggccc caccaccacg tcaacaggtt ccaaacgatc
     8401 gcgccgacga ggccgcacgc gacgacccac acgaacgcat gatcggagcc ttcgatcgtg
     8461 atgatctttg cgatcgtgtt cgcgacgccc tgatgaaaca cgaagagcgc gacgaagtta
     8521 aagatggtcg cccacaggac cgcgacgcga ggtgacaaga ctcgtgtcga gacgacggtc
     8581 gcaatcgaat tcgccgcgtc gtggaaccca ttgatgatgt cgaacagcag cgccaccgcg
     8641 atggtgacgt acatcgtggt ggtgacgtcc atcaggtgtg ctcgaggatc gtgccttcga
     8701 tgatgttggc gacgtcttcg cagcggtccg acgccatctc gaggttctcg taaacctctt
     8761 tccacttgat gacgaggatc ggatctttct cgtctttgaa caggcgagcg acggagcggc
     8821 gaaggatcgc gtcagcctcg ttctcgagac gattgatgtc gatgcagagc ttcagcgtgg
     8881 cctgaggcgt cttgagctgg cgcaggccgc gaacggcttg ctccacctgc agcgacgaac
     8941 ggtgcaggac gtcggcgaga tcgcgaacgt cggcggtcat cgtcgtcagc tcgtagagct
     9001 cgatgcgctc ggcggcggcc tcgacgtaat ccatgatgtc gtccatgcgc gtgatgaggc
     9061 gatggatcga atcgcggtcg attggcgtga tgaacgtctt gtgcagcgct tcgacgcagc
     9121 ggtgcgtgat cgtgtcggtc tcgtgctcga tgtccgagat gcgccggcac tttgccggga
     9181 tgttcgcacc ggtggtgacc atcgacagga actccttcgt gccttcgatc gtcagcccgg
     9241 catgctgctc gaagaagtcg aagaagctag tttcgcgagg caggaagcgt ccgaacatcg
     9301 caaggtcccc tgtaacacat gcgtgatcac ggggtcacgc gtgggaaaca accttcgctt
     9361 aggtatgtga caaccaggtg acgaacggca tctgaattca tgatccacgt gtgggtcgcg
     9421 tcgacttcgg caaatgccgc catctgatcg agcttcgtct cctcgacggc gacggtccca
     9481 tcgttcttca ccccgaccgg aaaccggcga ccgacggtcc agctcgtcac attcgtgagc
     9541 gcgaggctgc gcgttcctgc gatcacggcg ggcggcaccg gcggcagcgg ccatgcagtc
     9601 gcatcggcga gctcgagacc agccgggccg tagaagaact gaaacagcgg gttgcgtacg
     9661 agacccgcag cgagccggct gcctcgattc ggcggtgcga gcatcacgat gcggtcccag
     9721 cgcaggcgag gatcttgaag gtggcggacg atgactccgc ccatcgagtg cgtcaccgcc
     9781 gagaccggcc ggtcgcccgc cgcgtcgacg atctgctcgc tcagcgcgct cgcgaggtac
     9841 gcgatcgagt gctgcctcga tggataggtc gtgctccacg tgtcgaagcc gtgcttacgc
     9901 aggtgcgtcg acaacttcgc catcgagccg tgaccacgcg cgagcccgtg gaggagaacc
     9961 acgagtggct tcatgactcg ccgtctgcgc gagcctcggc ggcgtctttg tcgagctccg
    10021 catcgaggtc ggcgtcacgt cgggagcgcc acgattcctc gaggcgatcc gggtgatcgg
    10081 cgggaaacag cgattcgagc tcggcgggct tcaccgcgtt gaccgttgcg accacgtcgt
    10141 cggcgagctg ctcgcgttcg ttgcggagag cctgcacctc tttgcgaaac cgcagccggc
    10201 gaaacagcac gccgaggcca cgtcgcacga ggcgcaagcg atcgaggacc ttgagcgtcg
    10261 cgatgcccga gagcggcagg acgatcatcg cggcgacggc ccaccagagg ccagccttcc
    10321 accacagcac gccgatcgcc gctgcatacg cgagcagcac gagcatgagc ccgatgagca
    10381 gcttcgtcgt cgcgacgaca tctttgcgag gcgtgagacg tgggccagcg agccgcgcga
    10441 acaccagcgg cgggacgtgc agtgcggcgc cgggcgtcgt cagtggcaac cagaatgcga
    10501 cgagcacgag atgacgcagc acgcgcgcac tgacctcgag cttcgataga tcacgtgcga
    10561 gctctcgatc cgagaggccg agctcgtcga gcttctgctg atacgcgcgg acgcgctgca
    10621 tgagcgcaac cacgcgcgga tctgcggcga ccgcgccgta gtacgcgttg aagcgacgcg
    10681 cgagctcgac gcgatcttcg atcgagattt cctgcggctg gtagagccgg cgtacgacgt
    10741 cgagggcacg cacggtgtcc cagtcgggcg cgttgatcgt gagccgacgc ataccggtct
    10801 cgatctcgct cgtgagctcg cggacttcat cgggcgtgtt cgggcgcgca ttctcgacgg
    10861 tgatcgccgg tccgaactgg acgagcacgc ggctgcgaaa ccgcttcgga tgaatgaacg
    10921 tgagcccgca tggcacgatg tggatcggct ggctcgtgcg ttgtgctccg ccgagcgcga
    10981 gccgggcagc gccggtcttc agccgcgcga gctgcgactc gtcgtgcgag agcccctcgg
    11041 gaaagatgcc gatcgcgccg ccatcgccga gcacctggaa catcgcggtg aatgcggcgt
    11101 cgttgtcgac tggggccttc gtcgcaacgt cgggcttcga ctcgcgcgag ccgtcgtgat
    11161 cgtcgcggcg cttgatcggc acggcgccga ggccgttcag cagcgcgcgc atgatgcggc
    11221 ctttgaacag cgcgtccttc gcggcgaagt gaaccttgcg gccgcacgtc gtgatgatga
    11281 ggatcggatc gatcagcgag ttcgggtgat tgcccgcgaa caggaccggc ccgtccttcg
    11341 gcacgtgctc gaggccgacg acctcgatct ggcgaaagaa gacgcgtgtg acgacccgca
    11401 ggaaccacgc aaggatccgg tacgccaccg atctattgta cgcgttagcg cggctggaag
    11461 atgagtccgg cagtccgcaa gcgctcgagc agcgcgccgg ccattgccac ggacggcgtt
    11521 tgcacgccac cggcggactc gagctcgtcg tacgcgagac acagcgccga ttcgccgagc
    11581 atcttcgccg ttgacttgta gccaggatcg ccgtgtggat cggcgacgat atagaggagc
    11641 ttgtcgtcac catcctcggc gacgaagcgc actttccaat ggccgcgctc gcgcttctct
    11701 ggagatggtc cttcgcccgg ctgcggcgca cgcttcttca acatgctgcg caggcccggt
    11761 cgcttcatcg ctgccgagag cccggcgagc ccggccgtga ttcctgcggc catcaccgcg
    11821 ccgcgcggat tgcccggcgt gctcatcact tcgcgataga cgaagtcctc gccccatgga
    11881 tggcccgcga gcgcgtgacc tcgccgcacg acgggcgcat tcgtcgctgc catgaagaac
    11941 ggcacgaaga acatcttcag atacgagtcc cagccgacga ggcgctcgtc gcccttcgga
    12001 tggggaccat cgggatccgg atcgagcgca aacggattcc gcaggatgcg gcgcacctcg
    12061 gcgtccttca tcgcatcggc gatctcgaac gc
//
LOCUS       DATLBZ010000312        27726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8374023, whole genome shotgun sequence.
ACCESSION   DATLBZ010000312 DATLBZ010000000
VERSION     DATLBZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 27726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27726
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8374023"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VMZ53_16045"
     CDS             complement(<1..820)
                     /locus_tag="VMZ53_16045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30019.1"
                     /translation="MTEASEPSLERGAKVAGYEIEERIGIGGFGEVFRARQPVIGREV
                     AIKVLYAKYSSDPDAVSRFVAEARAVNKISHAGIVEVFDFGKLEDGRELYVMELLRGR
                     TLRDLLDDRGRIPLREAIPLLRGIAEAVDAAHKAGIAHRDLKPDNVFVLDDGRTKLID
                     FGLAKLMHEEDGATTRDGSVFGTPMYMSPEQCRGNAIDTRTDLYSFGVLAYHVLVGEA
                     PFTGDAIELALHHVNDRPVAPSKKNDELPRYVDHVLLALLAKDPIDRPASLGNAV"
     gene            complement(1044..2405)
                     /locus_tag="VMZ53_16050"
     CDS             complement(1044..2405)
                     /locus_tag="VMZ53_16050"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS30020.1"
                     /translation="MSETLSAIVSAGAKTRAQPALFIGLAADAPRAAGARLSLADIDR
                     VDLGRGETRAFTRRTADGARLLVAVLVDARMSTQHARITRLGTRWFVEDLASKNGTLV
                     RGARVARHELRDGDAICVGHTMLVFREAGGEGGDLQAAPAGEAPGLSTWSPALAARFA
                     ELATAARTNVPIEIMGPSGTGKELVARAVHALSSRRGKLAAINCGALPANLLEGELFG
                     HKKGAFTGAGDERAGLIRSADQGTLFLDEVAELPASSQAALLRVLQEGEVMPLGSDAA
                     IKVDVRLVTATHKNLDAEVAANRFRADLRARMLGVQVVLPELRERREDLGMLVATLLE
                     RLAPDRTVTFSSDAATALYTYDWPLNIRELERALAAALAIATDRVELHHLPAAVRQQD
                     ARPPATTIPEDLTADELALRESLSAAIARHDGNLAAVARELGKDRTQIRRWMKRFGLA
                     RED"
     gene            2453..3184
                     /locus_tag="VMZ53_16055"
     CDS             2453..3184
                     /locus_tag="VMZ53_16055"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUS30021.1"
                     /translation="MASEIAAIYRASKKKQDINWFTEWIARPPAAVVVYVLRNTRITP
                     NQITFGSAIVAAIACALFIAWPTWTGLIVAAAIFEFSFVLDCADGQLARHRKIASPIG
                     HLLDFLMDELKAMLLFGSVAIRLWRETGDDRMLLVGIAGGFCLASGIALTSFMRRPEY
                     GAKQMTEDGQPAEVGKRRGPVGLVLNTFEWAARFVVHYPQYLFICAAFNRIDIYFWAY
                     AGVNVLYLAMSLLKILLRLGRFAPP"
     gene            3181..3999
                     /locus_tag="VMZ53_16060"
     CDS             3181..3999
                     /locus_tag="VMZ53_16060"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HUS30022.1"
                     /translation="MTDVIRRAVLIAAGRGKRLGTHTDEVPKCMVEIGGKPILGHVWD
                     ALSSVGVEELVVIRGYRGDVLEKFIRSFIKNVIFVDNHDWQTNNVLLSLATARSFLDR
                     PTYLTYSDIVFTREVAEAAASSAAHIGLVIDRKFRDIYDGRTEHPLEEGEVADLLPDG
                     TVARVGKKALPPAEAVGEFIGLTRLGERGASIVARTLDQLSIRYIGREDEPYQRAARY
                     RNAYLTDLLQDLIDSGIQIDPIFIDGQWREIDTGQDLERARQLLESRPKEWTTP"
     gene            3996..4532
                     /locus_tag="VMZ53_16065"
     CDS             3996..4532
                     /locus_tag="VMZ53_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30023.1"
                     /translation="MKRLSVIAIAAFAALAALAGCNKPSEESCRKALANMQRLMGTEN
                     LRDDAALEGEVRRCKGGSKKAAVDCAIKAQTFEELKHCNFYHLPDNVLPSNLRNGSGA
                     ADTGTGTMTGSGTGTMSGSATMSGSGTMSGSGTTSGSGTMSGSGTASGSGTMSGSGTM
                     SGSGTMSGSGTASGSATH"
     gene            4832..5917
                     /locus_tag="VMZ53_16070"
     CDS             4832..5917
                     /locus_tag="VMZ53_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30024.1"
                     /translation="MRIGLRAVVAGAILASAALATTTLSRAHAESAVLAGIATPAPGD
                     ARKAIAVGPQGQVYEPDGKGAWVRKKAGGTGDEIIGAQSANGTIVADAKGSSMFRLED
                     NGWTSIRVAQKGGAILGAGTRLLAAAGKTIFALDKRSPTKLAEAPLPVLAVAGTIGGG
                     VVVATAKGFFGLQGTAFKQIKNAPRGGTLLSDRFLLVSNGVFDLKTQRTLTWPAGLKI
                     TDTALVGADMLVAVGSRGKTFELVTVKASGAVVVAKKGAAKGAKGAAGGGAAGGGAAG
                     GGAAGGGGADTETIPLPNASRVVGLVADKAGRVVVATRDGHIAVREVTGGASGASGAA
                     GGANWTVSEVREELPEAKPGPAPAEVP"
     gene            6159..7238
                     /locus_tag="VMZ53_16075"
     CDS             6159..7238
                     /locus_tag="VMZ53_16075"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HUS30025.1"
                     /translation="MTTAKPEKHVASHVFNVVVLAVGGVALAWMLHKLGWSTIREVMR
                     GVGAWFGVIVALDLAAIACEAAAIREFMRPEARMVKYWRVLAAQLSGRAINILTPGGA
                     LGEATKVTMLVSSAPRGRVVSSIVLVNLAAFYLSVTIVIVGVPLTALLVDLPPSLKMT
                     VWVGLGVVIAIVIGIGVLIQRGAFQTVLAGARGVGAVSRERAEKWSAKLAEIDRHVRE
                     LQSDRSPGTRAGLLFVCAARLCSWTATGVVLHAVGVEIGFVLLCGVLSVGVLIGWISS
                     IVPFGMGVADGGNYALFKVLGATGAHGVFVTLLGRARSLTLALVGLLVMVGGHSANRW
                     EIARRARLMARLEAEHGAESPVRSG"
     gene            7325..8152
                     /locus_tag="VMZ53_16080"
     CDS             7325..8152
                     /locus_tag="VMZ53_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS30026.1"
                     /translation="MAPHVLVADDDAWILRMVATVLEKRGYSVETAVDGEDALQRALA
                     RTPDLLITDVMMPKLDGWSLVRQLRSHTELAMLPVIFLTALSSEDDRIRGFRLGADDY
                     VTKPFRFEELDLRVAKTLRRTAQTVQDTREQLAGSGLRGDLSQVGLSSLLILIEMERK
                     TGLLQLRAPNGPTAQVLVREGKVVHARLEEQEEPVDAECVYYLLTWGAGEFEFIACVV
                     EGVDRVSVSTTHLLMEGARLMDEANEPSLAAAVSIPPKPDAPAAKGEPSETTDDGWD"
     gene            8250..8714
                     /locus_tag="VMZ53_16085"
     CDS             8250..8714
                     /locus_tag="VMZ53_16085"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HUS30027.1"
                     /translation="MSTNLEAQLREQLTAAIKAKDMKTANLIRMINTKVMERRTAKGF
                     TGTVDDALILDVIGTYKKSMEKAKQEFAAAGERGKEQIAEIEFEVEWCQKWLPQQVGE
                     AELREAVAKVVGELPQKDPKMAGRVIGAIKKQFGDRADAQLTKKIAEELLSG"
     gene            8812..8982
                     /locus_tag="VMZ53_16090"
     CDS             8812..8982
                     /locus_tag="VMZ53_16090"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5670 family protein"
                     /protein_id="HUS30028.1"
                     /translation="MLIALAVLLAVAWVLGWTVFHVASAGIHLLIALAVIALIVHFVQ
                     GARGGRGTLAPR"
     gene            complement(9093..10628)
                     /locus_tag="VMZ53_16095"
     CDS             complement(9093..10628)
                     /locus_tag="VMZ53_16095"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30029.1"
                     /translation="MRIACIHIPQFALQCATRLDPSLRGAPVAVVSGVEPGRERAGVL
                     HAPVVLACSRAAWALGVRLGMTAVTARSQSEQLAIVTADANAERETTRAIADAVLAAS
                     ATVDVGGRVGAGGAHLAMYCEVPGKTRGSSFGDKLVELLAELGLAARIGIADDRFTAW
                     VAAAYGPPNKQAKSTQEHQHDHQDGTVISVPRGGSAAFLAPRPLSLLAIPAEVQHMLE
                     SLGVTTLGEFAALPAPSVARPFEADYQGLARGESGATLRTYHPEAAIREEIVVSASTV
                     LELTGSLSGPAAIAQLARRIAVRLAGRGRGAARLDVAMLAADGELREVPLTIDGAIAD
                     AEEIARVLAPVLDTAQEDMPQLASHGGWRLRVVVTGEAIIGADGGGEAIEILAEGTAP
                     FVRVTPGETAPQQAAGIPNPALPLGPPPHMPRRDAANGNASSNRTHAREDRQIRLDSY
                     QADPDALSLVLSSSGSLFALSAPSGESDRRDAHRRTRRGKQRRSRPTPPVQRRLFDRL
                     GSK"
     gene            10741..11358
                     /locus_tag="VMZ53_16100"
     CDS             10741..11358
                     /locus_tag="VMZ53_16100"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HUS30030.1"
                     /translation="MRWLVVALLIAGCREPAPRKLDPPPPPGAARGTFALTYYWMSTP
                     TKPGIADTKLYAKDCSVLATVSAAYAKEMTETGNGRLVDGRTLVVAGECACSRSPCFR
                     DETVNLWGTGAGGRALVPFRSMAVDRSVIPLGTRLWIEELDGVAFPGAPLALHDGCVI
                     ADDTGGKIKGDKLDFFVGDEASYRDLDRALKLESVTVHDAGARCP"
     gene            11396..11800
                     /locus_tag="VMZ53_16105"
     CDS             11396..11800
                     /locus_tag="VMZ53_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30031.1"
                     /translation="MLARIALVCLVVATAPVVAGPAVEIHASCAADVATDPTHVRTLK
                     SELAHAMANATAAHAVDVTLVKLDTSSASQGEVIVRAEVRAFVSEPNGKVKWAASARA
                     TARGPAKDRSLVQRDAVTAVAQEIAKTIQKGR"
     gene            complement(12063..12887)
                     /locus_tag="VMZ53_16110"
     CDS             complement(12063..12887)
                     /locus_tag="VMZ53_16110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30032.1"
                     /translation="MAPLASGGMGGVYLARHVTSGEQVALKVLDPLFANHPEVVARLY
                     SERAIATKATHPGLISIRGAGKSSDDIPYLVMEYLEGRTLAQLLEAGLDVFDIVGLGA
                     QIAAALAALHEARIIHCDVKPENVLVLEKRAWGTLPQVKVIDFGVSRSIDEPLPDDAS
                     IAGTPAYMAPEQWKGRPTFASDVYSLGCMLYELLTGETPFDGSLPQLMHAHLSQRPAR
                     LTWLRTGIPIELERLILRALAKDPTSRPTMADLATELGDLADLMAATETMPMAMAV"
     gene            complement(13171..14868)
                     /locus_tag="VMZ53_16115"
     CDS             complement(13171..14868)
                     /locus_tag="VMZ53_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30033.1"
                     /translation="MRNWILVVAALSAVGCKKNKEADKAATPPAPGTSAPKAPVSTAD
                     QDALWALAPDGMAVGVVVSPAGMAKIEAGGLELKKLLDSPELAQFKAKADESLMEVLG
                     TSNPSLASAGLSAQKGFALFAPDNGDPIVILPVADRDKFLAVVKGQKGTDSDTIKKAT
                     CKTVKNVYACAKDVALFDRLGKGSFKDQLAIVAARGELELAGTITSKTGKPPATIAIV
                     GQLDRGIVVLRGAVKGLPDDAKKFFGNAKVRQDGDKTAGYALANLPPLVASLADQVPA
                     EEVLPGVKANQLVKSIGGPLTVTIPNGSPIFDLRLPLNDAAPMQTLIDQSASLPPMQM
                     FGATVKDHVLHVTIPQMLMSFDAWIENKELRIGTKGGAAPTATAPMGAIGQELAASEW
                     MFAMYGHGTLLAAPALPMPPLDQLPPEAALGFRALSLVTEAGFGMKIDGDTLRFVASV
                     RTIWANPDDVVKKVLAIGPKEITSGQFPGMAKAIVDASPKSAFAADYNAGYGALMMPA
                     AAIGMLAAISVPAFMKYQMRESMKEADDMQKDMDKQIKDAEDELKKHSGDDPPPPSP"
     gene            complement(14890..16392)
                     /locus_tag="VMZ53_16120"
     CDS             complement(14890..16392)
                     /locus_tag="VMZ53_16120"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUS30034.1"
                     /translation="MRSWVFLVGLLLASGNAAADKAGYDPKAIYKVPIGPNTVVGPAD
                     APITIVAWSDYACGYCNRVQNTLMHLEQLYPGQLRWVHRTLPLDDEYTLAAEASLAAA
                     AQGKFKPMHSRLYQLRGRVTRAETELVARELGLDMTKFRGDLDAGTYRKQIEADIADA
                     EKLGVTGTPAFFINGRAVIGSESLKLFAQIVDEELARAAAAKAAKPASLYDALVGQGR
                     TGADTEESEPPRDELDPASAYRIGLGLPGHQLGPDDALVTIVEWSDFQCPYCAKQAPV
                     FEHVRQKYGDQVRIIYRHLPMSFHRDAMLAAEAGAAAAAQGKFWPFHDELWKNFGHLA
                     RADLEAAAQAAGVEMKAFRQALDERRYHDAIVAEAAAAEALGVDGTPTSFVNGLPIVG
                     SRKIEDMDKIVDAHIANAKAMIAKGLPKAEVYPMFMAGAQGSDRADPSAVPETQQVHI
                     AMHAEDRGRAVAAACRRRDAARAATMASGLEGDVKKRTAAVCAGEGIDLK"
     gene            complement(16392..16856)
                     /gene="tsaE"
                     /locus_tag="VMZ53_16125"
     CDS             complement(16392..16856)
                     /gene="tsaE"
                     /locus_tag="VMZ53_16125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HUS30035.1"
                     /translation="MTAPVRLVDADATRHAGARLAAQLQGGDAIALIGDLGAGKTTFV
                     TGLVEALRGGTATSPTFSLVNEYRGGRLVVWHVDLYRLEREAELIELGLEEIVGNSSG
                     VVLIEWADKFPDVLPRDHLRIELTHDGDTRTLVASGSGSRGQALAADMLAFA"
     gene            complement(16853..17266)
                     /locus_tag="VMZ53_16130"
     CDS             complement(16853..17266)
                     /locus_tag="VMZ53_16130"
                     /inference="COORDINATES: protein motif:HMM:NF017719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone"
                     /protein_id="HUS30036.1"
                     /translation="MAAGEEVVDHKARMAEYLDRFGRERNLSLPPLDAEGVGTVQRGS
                     AVVSIHVLDSKGVLLLLARVAKQPKLDEARMKKLLMASFTDTNDAAFALHPQTGDLYL
                     RILRGLDGLDYEEFEDSLHSIAQTADHWDDKIADL"
     gene            complement(17279..18214)
                     /locus_tag="VMZ53_16135"
     CDS             complement(17279..18214)
                     /locus_tag="VMZ53_16135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30037.1"
                     /translation="MDRIGNYRIDRERRRDADSVVYEARHLVLPRRAFVKVMHEVSPQ
                     ALAVQLLREACILEALDHPGSVRVYESGLIPERRAWFAYERVDGSTVRDMLLNGPLDP
                     AAAAAMLRDIAEILDHAHRRGVVHCGLKPENIVVAGRTRGFPLCITDWSDARAHDTSA
                     PARLFGPADYTAPELVNGDAVDDRADIFALGVIAYQALTGDMPFDGVWIATVADGSTQ
                     HVPTEVRCPDAPPQLCQLVDQMLAWDRFDRPSSFEAKAELAQLAADISEPHPQSAGVV
                     RIRKPKWTPSLPLDGDAELIAIVTSDDDDKPTKSS"
     gene            18360..20297
                     /locus_tag="VMZ53_16140"
     CDS             18360..20297
                     /locus_tag="VMZ53_16140"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HUS30038.1"
                     /translation="MKRLPPKDQTPALRGGGTAKSPRIASYKIEARLDASRHSLQATQ
                     TLIWTNAGQSAVDRLPFHLYMNAFKSESSLFMQSTHGEMRGAKSTDSGWGWISIESVQ
                     IAGTELANKLEYPLAAQGDETVAELPLVTAIEPGQTIEVAFRFSVQLPEVFARTGYKG
                     DFHIVAQWFPKIGVRIGPPGAEQWECQPLQATTEFFADFGNYDVTLTVPNTEVVAATG
                     VLVGAIDQPGQMRQYTYHAEDVHDFVWMADPYMEVHKGEAKLDDGKVEVRVYARKEQA
                     MYAKRHLQAGIGAIEKFSAYFVPYPWPIMSIIDPPVDAWMGAGGMEYPTFVTTAGDTV
                     LARPGLRLPEYVTVHEIGHNWFQGILASNEAEEAWLDEGINEWADSHVMRDLYGPRTS
                     ATDWMGWQAEVDSLRAAIADDPEGIPSPIASAAYAFVDTDAYGEATYTSTMRALATLE
                     NLVGSSKFMAAMKVYAKEFAFKHPTARDLYTVLQRELGEDLGWFFQPVFQQVGGSKLS
                     VRSADCRQNHKVRGVEGDGPGRKTFTETERPDLGTFVCDVVVQNPGIIHIPVDVELRF
                     ADGSSQRVTWDDRGGSHWKRWTVERSSKLVEVRIDPDHKILLANPTTNAMRLEGDGSA
                     SLRASARIASWSQLLMQIVGP"
     gene            20297..21223
                     /locus_tag="VMZ53_16145"
     CDS             20297..21223
                     /locus_tag="VMZ53_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30039.1"
                     /translation="MATRLGLIDLFRAAPRSIGRYTGTLLAVFVVQTIVTLAVMMGVA
                     IVLAQAFGKLPIFDDAVDGDLVSLIWCLRFGKANFLAVIGIVLGGVLLWQLASWFLVG
                     GLYGVLANRPEGRRETARCFGASGTTTYLAYARLALCSIPGWFVVLSVFAQSLLLVEK
                     RLEYALTIPQLAGALAIAFLPALLVLHVLWTISDYARVELTLRGESHEPSVVMTYLRT
                     AMYVLKRPATLAHAGIGWLAFVLVTVIYAYIAQGHPMYGAEGAIALFVIREGVALART
                     AIRFGVLAGQIELGKTRALPPMHVKVDAKKSG"
     gene            complement(21220..22104)
                     /locus_tag="VMZ53_16150"
     CDS             complement(21220..22104)
                     /locus_tag="VMZ53_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30040.1"
                     /translation="MSRFFALALLLSGCDMLFQLDHLRVTADAPDAMIVRPDGALPEW
                     SQPTPLTGLNAPNTSAKYPYDSDASMTRDGLEIFFSSDRGNASGQFDIYHATRASVTA
                     SFGAPTVVTELSTSSGEAGQVNADGTELYFTRMGNNGVFKSTRPSGGTWSNPTKTTIM
                     PTIDVGNPAISGDGLQFVVNARVGTSDNDLYVLSRETQMDPWGPERHVVEVASTSEDG
                     AGCLDQDGLVMFFHTERMAGVRQIYETTRPNVADAFLFTSTYPAFEGDSTGSDPWISA
                     DRRTMVFSRDGDLYISTR"
     gene            22159..22803
                     /locus_tag="VMZ53_16155"
     CDS             22159..22803
                     /locus_tag="VMZ53_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30041.1"
                     /translation="MRNLTCAPMTYLLLLVASTAACGGDDEIDSDEQARRAYLCLDGS
                     IQKSIDLGFAGFSAASSANIPEQTGAGDVAGTIKVNGQVDQGSSNNKGMRLNVGMVGY
                     DDGPVEYNPDHDKVHVVFDTATTVADQPYLSLQLKNIPSGTLDGTLTSNSSMHGIYTL
                     SGDIQGTLTLNLTIAGPIMAGSGNVITVRVPGMTTVTGTATNSDGGVYNISITI"
     gene            22831..23256
                     /locus_tag="VMZ53_16160"
     CDS             22831..23256
                     /locus_tag="VMZ53_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HUS30042.1"
                     /translation="MSNAPTLESLLQDSGLADKAQVLEPNLVRLQWGSAFVIVGLSGS
                     AVVAIAPLFREIPRDKVTAFYKKLLEHNAYMGGMASFAIQTDGWVILHAGRALKGIDG
                     HEFANLVAGVGRFADQFDDQLINEFYVDHQEATNQADVQ"
     gene            23331..26672
                     /locus_tag="VMZ53_16165"
     CDS             23331..26672
                     /locus_tag="VMZ53_16165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS30043.1"
                     /translation="MPELTDADLEALTDELAWVRRLALSLVRDRSDADEIAQDTWLAA
                     KQHAPRDRPLRPWLSRVVLNLVRMRARSDARRVKREAAVVVEDHAPSPAELVDRVELQ
                     RTLVDAVLALAEPYRATVLLHFVEGLSCAEIARRFQIPDGTVRRRLKVALDNLREQLV
                     PDKSRSRLWAVLTPLVGSPYAAGRIAMKKLVAVIVVVAVVALGIVFVIGRDKPKPKAG
                     AAGDKPASGAVVNGGMLDGDAQVKPWFSQRGASPRRVAGKVLFEGAPVAGAIVRLADH
                     HELAAITTAKDGVFDFGMQPPAELSVSAEAADKTPAALLVPLADPNVKSENIILELTA
                     CNAKLHGSVVDASGGGIAKAHLRVAAFGGAESNAAGEYSLCVPMGDSVVRIDADGYGG
                     LALPIHLVGAHHRDFELVPEAELAGKVVDEEGSAVPHARILAVPQAIETPHFLSSGYA
                     MADADGAFRISGLAPGRFNLLANADGFSSTSPTAAVALAGTGNKSVTLVVGIRTSISG
                     RVVSGDKPVAGARITLAGSGIRFVRPSYSQKDGSFVLEGVPAGRARLDAGTYEVVSPK
                     ALVVPKTGMKDVKIEVAELATLRGHVTRKGEPVSGAIVQTNVGPKAQADQAGYYEIKG
                     LRPGTVQVTAQAFGAVNAFAPFTPVKLAGNAPTDHDIDLTGAAEVRGVVVDESGAPVP
                     NVYVRLIEARGDLGESMTDAQGRFTCTSMLGNSDYRAAVFPSPGARVAFPPAIDYPVL
                     KIADGNTILDGVKIAIKNQRLSIAGHVIDAEGNPVSDVHVEAIGRGFAAGPAMLPSVR
                     ADQGGGFVIKDLAPGTYTLHAHAGDGSDVQIPDVAAGAANVQVRVERPATIEGDLVGF
                     ASTPRVHARQMTADLALNNEATLEGNHFTISGLAPGRYVVEALGENENDGETVELKAG
                     AVLKVNLESHGRGTVEGIVTELGTGAPVPNMNCFAALSMNGLSGDIGQNAPVNDDTTT
                     NARGEFKIASPVGMARVMCFSADNSFAVAGADIKVAASTPTKAQLVAVRANPPPSDPG
                     FRIRPMTLPLVIAAVDPGSPAATAGLKMGDVVLSIDGTSVKGLIPSGAMMLAWNHKPG
                     TTIAVGVDRGGKQETISFAVRAPTN"
     gene            complement(26669..>27726)
                     /locus_tag="VMZ53_16170"
     CDS             complement(26669..>27726)
                     /locus_tag="VMZ53_16170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS30044.1"
                     /translation="QQLAQAKFATLVVDEAQALKNPTTQRAKAARALDADFRIALTGT
                     PLENHLGELWSLFSIVFPGLLGSWDQFRERFAAPIEKVKDPEARAALARVIQPFLLRR
                     TKAEVARELPSRTEVEVPIALSDDEHQLYEDARLAAVAELTIKGGGMRDEQRRFQVLA
                     ALTRLRLLASHPKLYDSASTIASSKLKRAIELLDELRSEGHRALVFSQFTSHLALVRE
                     ALDAHGFSTLYLDGSTPAKDRASLIDRFQAGEGDAFLISLKAGGTGINLTAADYVIHL
                     DPWWNPAVEDQATDRAHRIGQTKPVTVYRLISRGTIEDKIVALHRDKRALVAGVLEGT
                     DVAARLTAKDLMSLLSD"
BASE COUNT         4601 a         9025 c         9400 g         4700 t
ORIGIN      
        1 acactgcgtt gccgagggac gcggggcgat cgattggatc cttggcgagc agcgctagca
       61 ggacgtgatc gacataccgt ggcagctcgt cgttcttctt cgagggcgcg acgggccgat
      121 cgttcacgtg atgcagcgcg agctcgatcg catcaccggt aaacggcgcc tcaccgacga
      181 ggacgtgata ggcgagcacg ccgaacgaat acagatcggt gcgcgtatcg atcgcattgc
      241 cgcggcactg ctccggcgac atgtacatcg gcgtaccgaa gaccgagcca tcgcgtgtcg
      301 tcgcgccgtc ctcctcgtgc atgagctttg cgaggccgaa gtcgatgagc ttggtgcggc
      361 cgtcgtcgag cacgaacacg ttgtctggct tgagatcgcg atgcgcgatt cccgccttgt
      421 gagcagcatc gaccgcttcg gcgatgccgc gcagcagcgg gatggcctcg cgcagcggga
      481 tgcgcccgcg atcgtcgagc aggtcacgca gcgttctgcc gcggagcagc tccatgacgt
      541 agagctcgcg accgtcctcg agcttgccga agtcgaacac ctcgacgatg cccgcatgcg
      601 agatcttgtt gactgcacgc gcctccgcga cgaagcggga cacggcgtcc ggatcgctgg
      661 agtacttcgc gtagaggact ttgatggcga cctcgcggcc aatgactggc tggcgcgcgc
      721 gaaacacctc gccgaatccg ccgatgccga tgcgctcctc gatctcgtag cccgcgacct
      781 tcgcgccacg ttccagcgac ggttccgacg cctcggtcac cgccgccatc ttcgcacgcg
      841 agcgggacac gggactgtcc cgggacatga gagaacgcac ccaactccgc agttttccac
      901 gcagaccgac cggcatgcgg ggtgctgttg caaagcgcat gtcgaaagca gcggtgctgg
      961 cagtggcggt ggtcgcggtg ctcgtggcgt gtcggggaac gcgcgtgcgc gggtccggcg
     1021 gcggcgcggg cgccggctcg tactcagtcc tcgcgagcga gcccgaagcg cttcatccag
     1081 cgccggatct gcgtccgatc cttccccagc tcgcgcgcga ccgcggcgag attgccgtca
     1141 tggcgagcaa tcgcggcgga cagtgactcg cgcagtgcaa gctcatcggc ggtcaagtct
     1201 tcggggatgg tggtagccgg cggccgtgcg tcttgctggc gcaccgccgc cggtaggtga
     1261 tggagctcga cgcgatcggt tgcgatcgcc agcgccgcgg cgagagcgcg ttcgagctct
     1321 cggatattca gcggccagtc ataggtgtag agcgcagtcg ctgcgtccga cgagaacgtg
     1381 accgtccggt ccggcgcaag acgctcgagt agcgtcgcca cgagcatgcc gaggtcttca
     1441 cgccgctcgc gcagctccgg cagcactacc tgcactccca gcatgcgcgc gcgaagatcg
     1501 gcgcggaatc gattcgctgc cacctccgcg tcgaggttct tgtgcgtcgc ggtgacgagc
     1561 cggacgtcga ccttgatcgc ggcatcgctg ccgagcggca tgacctcgcc ctcttgcagc
     1621 acgcgcagca gcgctgcttg cgagctcgcg ggcagctcgg cgacctcgtc gagaaagagc
     1681 gtcccctgat ccgcgctgcg gatgagccct gcccgctcgt cgcccgcgcc ggtgaacgcg
     1741 cccttcttgt ggccgaacag ctcgccctcg agcaagttcg cgggcagcgc gccgcagttg
     1801 atcgcggcga gcttgccgcg cctgctggag agcgcgtgca cggcgcgagc gaccagctcc
     1861 ttgcccgttc ccgacggccc catgatctcg atcggcacgt tcgtgcgcgc cgcagtcgca
     1921 agctctgcaa agcgcgccgc gagcgcggga gaccacgtcg agagtccggg cgcctcgccc
     1981 gccggcgcag cctgcaagtc gccgccctcg ccgccggcct cgcgaaacac gagcatcgtg
     2041 tggccgacgc aaatcgcatc accatcgcga agctcgtgac gcgcgacgcg tgcaccacgc
     2101 accaaggtcc cattcttcga tgcgagatct tcgacaaacc agcgcgtgcc gaggcgagtg
     2161 atgcgcgcgt gttgcgtcga catccgcgca tctacgagga cagcgaccag cagccgcgca
     2221 ccgtcggcgg tccgccgcgt gaacgcacgc gtctcgccac gtccgagatc gacccgatcg
     2281 atgtcggcga gcgagagcct cgcaccggct gcgcgcggtg catcggcggc aagcccgatg
     2341 aagagcgcgg gttgcgcgcg cgtcttcgca ccggccgaca cgatagcgct gagcgtctcg
     2401 ctcatcccga tccaccttac actgctatgc gtcgtgctag cttcgcggcg taatggcatc
     2461 tgaaatcgcc gccatttatc gggcgtcgaa aaagaagcaa gacatcaact ggttcaccga
     2521 gtggatcgcg cgtccaccgg cggcggtcgt tgtctacgtg cttcgcaaca cgcgcatcac
     2581 gccgaaccag atcacgttcg gctccgcgat cgtcgccgcg atcgcatgcg cgctgttcat
     2641 cgcatggccg acatggacgg gcctgatcgt cgcggcggcg atcttcgaat tctcgttcgt
     2701 gctcgactgc gccgacggcc agctcgcgcg ccaccgcaaa atcgcatcac cgatcggtca
     2761 cctgctcgac ttcctgatgg acgagctcaa ggcgatgctt ctcttcggct ccgtcgcaat
     2821 ccgcctctgg cgcgagaccg gcgacgaccg catgttgctc gtcggcatcg cgggcggatt
     2881 ctgcctcgca tccggcatcg cgctgacgtc gttcatgcgc cgccccgaat acggcgcgaa
     2941 gcaaatgacg gaagacggcc agcccgccga agtcggcaag cgccgcgggc ccgtcggcct
     3001 cgtgctgaac acgttcgagt gggccgcgcg cttcgtcgtc cactatccgc agtacctctt
     3061 catctgcgcg gccttcaacc gcatcgacat ctacttctgg gcttacgcag gcgtgaacgt
     3121 cttgtatttg gcgatgagcc tgctgaagat cctgcttcgc ctcgggaggt tcgctccacc
     3181 atgaccgacg tcattcgccg cgccgtactc atcgccgcag gtcgcggcaa gcgcctgggc
     3241 acgcacacgg acgaagtccc caagtgcatg gtcgagatcg gcggcaagcc gatccttggc
     3301 cacgtgtggg acgccctgtc gtccgtcggc gtcgaagagc tcgtcgtcat tcgcggctat
     3361 cgcggcgacg tgctcgagaa gttcatccgg tcgttcatca agaacgtcat cttcgtcgac
     3421 aaccacgact ggcaaacaaa caacgtcctc ctctctctcg cgaccgcacg ctcgttcctc
     3481 gatcggccaa cgtacctgac gtactccgac atcgtgttca cgcgcgaagt cgccgaagcg
     3541 gccgcgtcct ccgccgctca catcggcctc gtcatcgatc gcaagttccg cgacatctac
     3601 gacggccgca ccgagcatcc actcgaggaa ggcgaagtcg ccgacctgct ccccgacggc
     3661 accgtcgcgc gcgtcggcaa gaaggcgctg cccccagccg aggccgtcgg cgagttcatc
     3721 ggcctcacgc gcctgggcga acgcggcgcc agcatcgtcg cgcgcacgct cgaccaactc
     3781 agcatccgct acatcggtcg cgaggacgag ccgtaccaac gcgccgcgcg ctatcgcaac
     3841 gcctacctga ccgacctcct gcaagacctc atcgattcag gaatccagat cgatcccatc
     3901 ttcatcgacg gccaatggcg tgagatcgat accggccagg atctggaacg cgcgcgtcag
     3961 ctgctagagt cgcgcccgaa ggaatggacc acgccatgaa gcgcctctcc gtcatcgcca
     4021 tcgccgcgtt cgccgctctc gccgctctcg ccggctgcaa caagccgagc gaagagagct
     4081 gccgcaaagc gctcgcgaac atgcagcgcc tcatgggcac cgagaacctg cgcgacgatg
     4141 cggccctgga aggcgaagtc cgtcgctgca aaggtggctc gaagaaggcc gccgtcgatt
     4201 gcgcgatcaa ggcgcagacc ttcgaagagc tgaagcactg caacttctat cacctgccag
     4261 acaacgtgct tccgtcgaac ctccgcaatg gctccggcgc cgctgacacg ggcaccggaa
     4321 cgatgaccgg ctcaggcacc ggaacgatgt ccggctccgc cacgatgtcc ggctctggca
     4381 cgatgtccgg ctccgggaca acgtccggct ccgggacaat gtccggctcc ggaaccgcgt
     4441 ccggctcggg gacaatgtcc ggctcaggca cgatgtccgg ctcggggacg atgtccggct
     4501 ccgggacagc gtccggctcg gcgacacact aagcgcagtt cggctcgacg aggatgtccg
     4561 gttcggggac agcgtccggc tttgggacac actaagcgca cgttccgctc gaggaggatg
     4621 tccggctcgg ggacagcgtc cggctcgggg acagcgtccg gcttcgggac aacgtccggc
     4681 ttcgggacaa cgtccggctc ccggcacacg gacgccgcgt ccggctgcgg gacatctacg
     4741 gggtgtccgg ctcgcgacat cgttcgcgcg ctcggccgag ctgcgcacgc agcgatctgg
     4801 atcggccggt agtcgcgggt atgctgaggg cgtgcgtatc ggacttcgtg cggtagtcgc
     4861 gggcgcgatt ctcgcgagcg cggcactcgc gacgacgacg ctgtcgcgcg cgcatgcgga
     4921 gtcggccgtg ctcgcgggca tcgcgacgcc ggcgccgggc gacgcacgca aagcgatcgc
     4981 ggtggggccg caaggccagg tctacgagcc cgacggcaaa ggcgcgtggg tgcgcaagaa
     5041 ggccggcggc accggcgacg agatcatcgg cgcgcagtct gcgaacggca cgatcgtcgc
     5101 cgatgcgaag ggctcgtcga tgttccggct cgaagacaac ggctggacat cgattcgcgt
     5161 cgcgcaaaaa ggcggcgcga ttctcggcgc cgggacgcgc ctgctcgccg cggccggcaa
     5221 gaccatcttc gcgctcgaca agcgctcacc gacgaagctc gcggaagcgc cgctgcccgt
     5281 gctcgcggtt gcgggcacga tcggcggcgg cgtcgtcgtc gcgacggcga agggcttctt
     5341 cggcttgcaa ggcacggcgt tcaagcagat caagaatgcg ccgcgcggcg gcacgctgct
     5401 cagcgatcgg ttcttgctcg tgagtaatgg cgtattcgat ctcaagacgc agcggacgct
     5461 gacgtggccg gccggcctca agatcacgga caccgccctc gtcggtgcag acatgctcgt
     5521 tgcggtgggc tcgcgcggca agacgttcga gctcgtgacg gtgaaggcga gcggcgcggt
     5581 cgtcgtcgcg aagaaaggcg ctgcgaaggg tgcaaagggt gcggcgggcg gcggtgcagc
     5641 gggcggcggt gcggcgggag gcggtgcggc gggcggcggg ggcgcggaca ccgagacgat
     5701 tccgctgcca aacgcatcgc gcgtggtcgg gctcgtcgcc gacaaggcgg gtcgcgtcgt
     5761 cgtcgcaacg cgcgatgggc acatcgccgt ccgcgaggtc acgggcggtg cgtccggtgc
     5821 gagcggcgcg gccggtggcg ccaactggac cgtcagcgaa gtgcgcgaag agctgcctga
     5881 ggcaaagccc ggccccgccc cggccgaagt gccgtgagca ccgccaagcg atcgaacgtc
     5941 tcggtcgagg cgagcgaagc aacgcaggag tgcgtcggcc gcagtgccgc gagcggccgg
     6001 agtgccatga gcacggccaa gcgatcgaac gtctcgccag tcgaggcgag cgaagcagtg
     6061 ccggcgagag cgcgccggtc ttcccgcacg agaagagttg cggtcgtgac gacggcgaag
     6121 gagtgtgccg gtggtcccgc tcgagaggcg ttgctgtcgt gacgacggcg aagcccgaga
     6181 agcacgtcgc gagtcacgtg ttcaacgtcg tcgtgctcgc ggtcggcggc gtcgccctcg
     6241 cgtggatgct gcacaagctc ggttggtcga cgatcagaga ggtcatgcgc ggcgtggggg
     6301 cgtggttcgg cgtcatcgtc gcgctcgacc ttgcggccat cgcgtgtgag gccgcggcga
     6361 ttcgcgagtt catgcgaccc gaagcgcgca tggtgaagta ctggcgcgtg cttgctgcgc
     6421 agctcagcgg tcgcgcgatc aatattctga cgccgggcgg tgcgcttggt gaggcgacga
     6481 aggtgacgat gctggtgtcg agcgcgccgc gcgggcgcgt tgtgtcgtcg atcgtgctcg
     6541 tgaacttggc ggcgttctat ctctcggtga cgatcgtgat cgttggcgtg ccgctgactg
     6601 cgttgctcgt ggatttgccg ccgagcttga agatgacggt gtgggtcggg ttgggcgtcg
     6661 tgatcgcgat cgtgatcggg atcggcgtgc tgattcagcg tggcgcgttc cagacagtgc
     6721 tcgcgggcgc gcgcggggtg ggtgcggtca gccgcgaacg cgcggagaaa tggagcgcga
     6781 agctcgctga gatcgatcgg catgtgcgcg agctgcagtc tgatcgatcg ccgggcacgc
     6841 gcgccggctt gctgtttgtt tgcgcggcgc ggctctgttc gtggacggcg accggggtcg
     6901 tgctgcacgc ggttggggtc gagatcgggt tcgtgctgtt gtgcggggtg ctgtccgtcg
     6961 gcgtgctcat cggttggatc tcgtcgatcg tgccgtttgg aatgggcgtc gcggacggtg
     7021 gcaactacgc cctgttcaag gtcctcggcg cgaccggcgc gcacggcgtc ttcgtgacgc
     7081 tgctggggcg ggcgcgcagc ctgacgctcg cgttggtcgg gcttctcgtg atggtgggcg
     7141 ggcactcggc aaatcgctgg gagatcgctc gtcgcgctcg gctgatggcg cgcctggagg
     7201 ccgaacatgg cgcggaatca ccggttcgaa gcggctaacg ccccacccgt aaccgtggaa
     7261 gcgcgccaaa acccgccgca tccttgcgat gttgggcggg agcacgttac ctttggggtt
     7321 agacatggct ccgcatgtcc tggtggccga cgacgacgcc tggatcttgc gcatggtggc
     7381 gaccgtgctc gagaagcggg gctacagcgt cgagaccgca gtcgatggcg aagacgcgct
     7441 ccagcgcgcg ctcgcacgaa cgccggatct gctgatcacc gacgtcatga tgccgaagct
     7501 cgacggttgg tcgctcgtgc gtcagttgcg atcgcatacc gagctcgcga tgttgcccgt
     7561 catcttcttg accgcgctgt cgtccgaaga cgatcgcatt cgcgggtttc gcctcggcgc
     7621 cgatgactac gtcacgaagc cgtttcgctt cgaagagctc gacctgcgcg tcgcgaagac
     7681 actccgccgc actgcgcaga ccgtgcagga cacgcgcgag cagctggcgg gctccgggtt
     7741 gcgcggcgac ctgtcgcaag ttggtttgag ctcgctgctg atcctcatcg agatggagcg
     7801 caagaccggc ttgctccagt tgcgcgcgcc gaatgggccg accgcacaag tgctcgtgcg
     7861 cgagggcaag gtcgtccacg cgcggctcga ggagcaagaa gagccggtcg atgccgagtg
     7921 cgtctactac ttgctgacgt ggggcgcggg cgagttcgag ttcatcgcgt gcgtggtcga
     7981 aggcgtcgat cgcgtgagcg tgtcgacgac gcacttgctc atggagggcg cgcggctcat
     8041 ggacgaggcg aacgagccgt cgctcgcagc tgcggtctcg atcccgccga agccggacgc
     8101 gcctgccgcg aagggcgaac catccgagac caccgacgac ggctgggact agcgaccgcg
     8161 cggcgcggct ggaccaacgc ggcggcacgg cgcggctgga ttgcacaccg cggctgggat
     8221 tcgctgacgt tcagtggtaa gccgtcgcca tgtcgacgaa cctcgaggct cagcttcgcg
     8281 agcagctcac cgctgcgatc aaggcgaagg acatgaagac cgccaacctc attcgcatga
     8341 tcaacacgaa ggtcatggaa cggcgcacgg cgaagggctt cacgggcacg gtcgacgacg
     8401 cgctgatcct cgatgtgatc ggcacctaca agaagtcgat ggagaaggcg aagcaagagt
     8461 tcgccgcggc gggtgagcgc ggcaaggaac agatcgccga gatcgagttc gaggtcgagt
     8521 ggtgccagaa gtggctgccg cagcaggtcg gtgaggcgga gctccgcgaa gccgtcgcga
     8581 aggtcgtggg cgagctgccg caaaaagatc cgaagatggc gggtcgcgtg atcggcgcga
     8641 tcaagaagca gttcggcgat cgcgcggacg cgcagctgac gaagaagatc gctgaagagc
     8701 tgctatcggg ctaacgctgt cgcaactaga gcgcagttcg gttgccctcc caagatcccg
     8761 cagccggttc ggccacggat ctgcattccc ttccgctagg agggaatcat catgttgatc
     8821 gcactcgcag tacttctcgc agttgcatgg gttctcggct ggacggtctt ccatgtcgca
     8881 tccgccggca tccatctcct gatcgctctc gccgtcatcg cgctgatcgt gcacttcgtg
     8941 cagggcgcgc gcggtggtcg aggaacactc gcaccacgct gatcgcgagt aatcgcccca
     9001 cactggcgcg agtaatcgcc ccacggcgcg gtaatcgccc cacactggcg ctagtagtcg
     9061 cgccacgcta atggcgagga agagtcgccg agctacttcg atccgagacg atcgaagagc
     9121 cggcgctgca ccggcggcgt aggccgagac ctacgttgct taccgcggcg cgtgcggcgg
     9181 tgcgcgtcgc gacggtctga ctcaccgctt ggcgcggaca gcgcgaagag cgatcccgac
     9241 gacgacagca cgagcgaaag cgcgtcggga tccgcctgat acgaatcgag ccggatctga
     9301 cgatcttcgc gcgcgtgcgt gcggttggag ctcgcgttgc cgttcgccgc atcgcggcgc
     9361 ggcatgtgcg gcggcggtcc aagcggcagc gccgggttcg gaatgcctgc agcctgctgc
     9421 ggagccgtct cgcccggcgt gacgcgcacg aacggcgcgg tgccctccgc gagaatctcg
     9481 atcgcttccc cgccgccgtc ggcgccgatg atggcttcgc ccgtgacgac gacgcgcagg
     9541 cgccagccac cgtggctcgc gagctgcggc atatcttctt gcgcggtgtc gagcaccggc
     9601 gcgagcacgc gcgcgatctc ctcggcgtcg gcgatcgcac cgtcgatggt gagcggcact
     9661 tcgcggagct ctccatcggc tgccagcatc gcaacatcga ggcgcgctgc accgcgaccg
     9721 cgaccggcga ggcgaaccgc aatccgacgc gcgagctgcg cgatcgccgc ggggccgctg
     9781 agcgagcccg tgagctcgag caccgtgctc gcactgacga cgatctcttc gcggatcgca
     9841 gcctcggggt gatacgtgcg cagcgttgca ccgctctcgc cgcgcgcgag gccttggtaa
     9901 tcggcttcga acgggcgcgc gaccgacggt gcgggcagcg ccgcgaattc gccgagcgtc
     9961 gttacgccga gcgactcgag catgtgctgc acctcggccg gaatcgcgag cagcgatagc
    10021 ggacgcggcg cgaggaacgc agccgatccg ccgcgcggca cgctgatgac ggtgccgtcc
    10081 tgatgatcgt gctggtgctc ctgcgttgac ttggcctgct tgttcggcgg gccgtatgcc
    10141 gcggcaaccc acgcggtgaa gcgatcgtcg gcgatgccga tgcgcgccgc gaggccgagc
    10201 tccgcgagca gctcgacgag cttgtcgccg aacgacgagc cgcgcgtctt gcccggcacc
    10261 tcgcaataca tcgcgagatg cgcgccgccc gcaccgacgc gaccgcccac gtcgaccgtg
    10321 gccgacgccg caagcactgc atctgcgatc gcgcgcgtgg tctcacgctc ggcgttcgca
    10381 tccgcggtga cgattgcgag ctgttccgac tgactgcgtg ccgtcacggc cgtcatgccg
    10441 aggcggacgc cgagcgccca cgcagcgcgc gagcacgcga gcaccaccgg tgcgtgcagc
    10501 acccctgccc gctcgcgacc gggctcgacg ccggacacga ccgcgaccgg tgcaccgcgg
    10561 agcgatggat cgaggcgggt tgcgcactgg agtgcgaact gagggatgtg gatgcaggcg
    10621 attcgcatgc tcttagctct aactcactgg tctgatgttc agtatagcag acactccgga
    10681 ttgcgttcca ctccggaccg taaccacgta aaacgaaacg tctagtccta tgatctgctc
    10741 gtgcggtggc ttgtcgtggc tttgctgatc gcaggctgca gagagcccgc gccacgcaag
    10801 ctcgacccgc ctccgccacc cggcgctgca cgcgggacgt tcgcgctgac ctattactgg
    10861 atgagcacgc ccaccaagcc gggcatcgcg gacacgaagc tctacgcgaa ggactgctcc
    10921 gtgctcgcga cggtctctgc ggcgtacgcc aaagagatga cggagaccgg gaacggccgg
    10981 ctcgtcgacg ggcgcacgct cgtcgtcgcg ggcgagtgcg cgtgctcgcg ttcgccgtgc
    11041 tttcgcgacg agaccgtgaa cctctggggc acaggcgcgg gtggcagagc gctcgttccg
    11101 tttcgatcga tggcggtcga ccggagcgtg attccgctcg gtacgcgtct gtggatcgag
    11161 gagctcgacg gcgtcgcatt tcccggcgca ccgctcgcgc tccacgatgg ttgcgtgatc
    11221 gccgacgaca cgggcggcaa gatcaaaggc gacaagctcg acttcttcgt cggagacgag
    11281 gcgagctatc gagatctcga ccgcgcgcta aagctcgaga gcgtgacggt gcatgacgcc
    11341 ggcgcacgct gcccctgaaa cagagtgggc atccttcctg ctgatagtgg aaggtatgtt
    11401 ggctcgcatc gcgcttgttt gtctcgtcgt tgcaacggcg cccgtcgtgg ctggacctgc
    11461 cgtcgagatt cacgcgagtt gcgcggcgga tgtcgctacg gatcccacgc atgtgcgcac
    11521 gctgaagagc gagctggcac acgcgatggc caacgcgacg gcggcgcacg cggtggatgt
    11581 aaccctggtc aagctcgaca cgtcgtcggc gtcccaaggt gaggtcatcg tgcgtgcgga
    11641 ggttcgcgcg ttcgtgtcag aaccgaacgg caaggtgaag tgggcagcga gcgctcgcgc
    11701 gacagcccgc ggccccgcga aagatcgctc gctcgttcag cgcgacgcgg tcaccgcagt
    11761 tgcgcaagaa atcgcgaaga cgatccaaaa gggtcgctaa ccgcggcgtc cgaaacccgg
    11821 acgcgctccg tccctcgcgg ttgcggaagt tgcgaaggtt gcgaaggttg cggaagttgc
    11881 gtccgtccct tacgggtcgc tccgtccctc ggttgcggaa gttgcggagg ttgcggaagt
    11941 tgcgtccgtc ccttaagggt cgctaactcc gcgttcaaac agcggagccg cgatcgaatg
    12001 ccgatgacgc tccgtccctc gcggttgcga aggttgcgga agttgcgtcc gtcccttagt
    12061 ggtcagaccg ccatcgccat cggcatggtc tcggtcgctg ccatcaggtc cgcgaggtcg
    12121 ccgagctcgg ttgcgagatc ggccatcgtc gggcgagatg ttgggtcctt cgcgagcgcg
    12181 cgcaggatca gtcgctcgag ctcgatcggg attccggtgc gcagccacgt gaggcgcgcg
    12241 ggccgctgcg agagatgggc gtgcatcagc tgaggtagcg agccatcgaa gggtgtctca
    12301 ccggtgagca actcgtagag catgcagccg agcgagtaga catcggaggc gaaggtcggg
    12361 cgccctttcc attgctcggg tgccatgtat gcgggcgtgc ccgcgatcga cgcgtcgtcc
    12421 ggaagcggct cgtcgatcga gcgcgacacg ccgaagtcga tcactttgac ttgagggagc
    12481 gttccccatg cacgcttctc gagcacgagg acgttttcgg gcttcacgtc gcagtggatg
    12541 atgcgagctt cgtgaagggc cgcgagtgca gcggcgatct gcgcgccgag accgacgatg
    12601 tcgaacacgt cgaggccggc ttcgagcagc tgcgcgagcg tgcgtccttc gaggtactcc
    12661 atgacgaggt acgggatgtc gtcgctgctt tttcctgcgc cgcggatcga gatgaggccg
    12721 gggtgggtgg ccttcgtcgc gatcgcgcgc tcggagtaga ggcgcgcgac aacctcgggg
    12781 tggttggcaa acagtggatc gagaaccttg agagctacct gctcgccact tgtaacgtgc
    12841 ctcgcaaggt aaacgccgcc catgcctccg ctagcgaggg gagccacgac atcgtagtca
    12901 ccgaagcgcg aggaggacgg catagccagc cgacaactgc atcgcagatg ccacggacaa
    12961 ctgccgtgta ggcgttgaat gacgtcgcgt gtctggcggt tcttcaccgc agaaaactga
    13021 tatccgactg tggcgacgcg tccacaaacc gagcctgcga cgccacggga cagctgtcga
    13081 taaacgctgc agtcgcaact ggatagcagc tgtcaggaat ggcgagagaa cgtcgcgtcg
    13141 ggctcgcgtg tcagggcgac gtcggcggca tcacggcgac ggcggcggcg ggtcatcgcc
    13201 gctgtgcttc ttgagctcgt cctcggcgtc cttgatctgc ttgtccatgt ccttctgcat
    13261 gtcgtcggct tctttcatcg actcgcgcat ctggtacttc atgaacgcgg gcaccgagat
    13321 cgcagcgagc atgccgatcg cagcggccgg catcatgagc gcgccgtagc cggcgttgta
    13381 gtcggcggcg aacgcagact tcggcgacgc gtcgacgatc gcctttgcca tgccggggaa
    13441 ctggccggac gtgatctcct tcgggccgat cgcgagcacc ttcttgacga cgtcatcggg
    13501 gttcgcccag atcgtgcgca cgctcgccac gaagcgaagc gtatcgccgt cgatcttcat
    13561 gccgaagccg gcttcggtga cgagcgagag agcgcggaat ccgagggcgg cttccggcgg
    13621 aagttgatcg agcggcggca tcggcagtgc gggtgcggcg agcagcgtgc cgtggccgta
    13681 catcgcgaac atccactcgc tcgctgccag ctcctggccg atcgcgccca tcggagccgt
    13741 tgcagtgggg gccgcgcctc ccttcgtgcc gatgcgcagc tccttgttct cgatccacgc
    13801 gtcgaacgac atgagcatct gcggaatcgt cacgtggagg acgtggtctt tgaccgtcgc
    13861 gccgaacatc tgcatcggcg gcagcgaggc cgactgatcg atcagcgttt gcatcggcgc
    13921 ggcatcgttg agcggcaagc gcagatcgaa gatcggcgag ccgttcggga tcgtgaccgt
    13981 gagcgggcca ccgatcgact tcacgagctg gttcgccttc acgccgggga gcacttcttc
    14041 ggccggcacc tgatcggcga gcgatgcgac gagcggcggc aagttcgcga gcgcgtagcc
    14101 tgcggtcttg tcgccgtctt ggcggacctt cgcgttgccg aaaaacttct tcgcgtcgtc
    14161 ggggaggcct ttgacggcgc cgcgcagcac gacgatgccg cggtcgagct gaccgacgat
    14221 cgcgatcgtc gccggcggct tgccggtctt gctcgtgatg gtgccggcga gctcgagctc
    14281 accgcgcgcc gccacgatcg cgagctgatc cttgaagctg cccttgccga ggcgatcgaa
    14341 cagcgcgacg tccttcgcgc acgcgtagac gttcttgacc gtcttgcacg tggccttctt
    14401 gatcgtgtcg ctgtcggtgc ccttctggcc cttcacgacg gcgaggaact tgtcgcgatc
    14461 cgcgacgggc aggatcacga tcggatcgcc gttatcgggc gcgaacagcg cgaagccctt
    14521 ctgcgccgag agtcccgcgc tcgcgagcga cggattggac gtgccgagca cctccatgag
    14581 gctctcgtcg gccttcgcct tgaactgcgc gagctcgggc gaatcgagga gcttcttcag
    14641 ctcgaggcca ccggcttcga tcttcgccat gccggcaggc gacacgacga cgccgaccgc
    14701 catgccgtcg ggcgcgagcg cccacagtgc atcttgatca gccgtcgaga ccggcgcctt
    14761 gggcgcgctc gttccgggcg caggaggcgt tgccgccttg tccgcttctt tgttcttctt
    14821 gcaaccgacc gcagacagcg cggctacgac gaggatccag ttccgcatcc gccgcttgta
    14881 caacagctgc tacttcaggt cgatgccttc gcctgcacag acggcggcgg tccgcttctt
    14941 gacgtcgcct tcgaggccgc tcgccatcgt cgccgcccgc gccgcatcac gacgccggca
    15001 cgcagcagcc actgcgcgcc cgcgatcctc ggcatgcatc gcgatgtgga cctgctgggt
    15061 ttcgggcacc gccgacgggt ccgcgcgatc gctgccctgc gcgccggcca tgaacatcgg
    15121 atagacctcg gccttcggca ggcccttggc gatcatcgcc ttcgcgttcg cgatgtgcgc
    15181 gtcgacgatc ttgtccatgt cttcgatctt gcgcgacccg acgatcggca gcccgttgac
    15241 gaacgacgtg ggtgtgccgt cgacgccgag cgcttcggcc gccgccgcct ccgcgacgat
    15301 cgcatcgtga tagcgccgct cgtcgagcgc ctgccgaaac gctttcatct cgacgcccgc
    15361 cgcctgcgcc gctgcttcga gatccgcgcg cgcgagatgc ccgaagttct tccacagctc
    15421 gtcgtgaaac ggccagaact tgccctgcgc cgccgctgca gcgccagcct ccgccgcgag
    15481 catcgcatcg cgatgaaacg acatcggcaa gtggcgatag atgatgcgca cctgatcgcc
    15541 gtacttctgg cggacgtgct cgaacaccgg cgcttgcttc gcgcagtacg ggcactggaa
    15601 gtcgctccat tccacgatcg tgacgagcgc gtcgtcggga ccgagctggt gaccgggcaa
    15661 gccaagcccg atgcgatacg cgctcgcggg atcgagctcg tcgcgcggcg gctcgctctc
    15721 ttcggtatcc gcgcccgttc ggccctgccc tacgagcgcg tcatacaagc tcgccggctt
    15781 cgccgccttt gccgccgctg cccgcgcgag ctcttcgtcg acgatctgcg cgaacagctt
    15841 caatgactcc gagccgatca ccgctcgccc attgatgaag aacgccggcg tgcccgtgac
    15901 gccgagcttc tcggcatcgg cgatatccgc ctcgatctgc ttgcgatacg tgcccgcgtc
    15961 gaggtcgccg cgaaacttcg tcatgtcgag gccgagctcg cgcgcgacga gctccgtctc
    16021 cgcccgcgta acgcgaccgc gcagctgata gagccgcgag tgcatcggct tgaacttgcc
    16081 ctgcgcggcg gccgcaagag atgcctcggc agcgagcgtg tattcgtcgt cgagcggcag
    16141 cgtgcgatgc acccagcgga gctgccccgg atagagctgc tcgaggtgca tgagcgtgtt
    16201 ctgcacgcga ttgcagtagc cgcacgcgta gtcgctccac gcgacgatcg tgatcggcgc
    16261 gtctgccgga ccgacgacgg tgttcggccc gatcggcacc ttgtagatcg ccttcgggtc
    16321 atagccggcc ttgtccgctg ccgcattgcc gctcgcgagc agtaagccga caaggaacac
    16381 ccaggagcgc atcacgcaaa agctagcatg tcagccgcca gcgcttgtcc ccgactgccg
    16441 ctccccgacg ctactaatgt ccgcgtatca ccgtcgtggg tcagctcgat tcgcaggtgg
    16501 tcgcgcggca gcacatcggg gaatttgtcg gcccactcga tcagcacgac gccgcttgaa
    16561 ttgccgacga tctcctcgag gccgagctcg atcagctctg cttcgcgttc gagccgatac
    16621 aggtcgacgt gccaaacgac gaggcggccg cctcgatact cgttgacgag cgagaacgtc
    16681 gggctcgtgg ccgtgccgcc gcggagggct tcgacgaggc ccgtcacgaa cgtcgtcttg
    16741 cccgcgccga ggtcgccgat cagcgcgatc gcatcgccgc cttggagctg ggccgcgagc
    16801 cgcgcgccgg catgccgggt agcgtccgca tcgacgaggc gaactggcgc ggtcacaggt
    16861 ctgcgatctt gtcgtcccag tggtcggccg tctgcgcgat cgagtgcagc gagtcttcga
    16921 actcctcgta gtcgaggccg tcgaggccac gcaggatccg caggtagagg tcgcccgtct
    16981 gcgggtgcag cgcgaacgcg gcgtcgttcg tgtccgtgaa cgatgccatc agcagcttct
    17041 tcatccgcgc ttcgtcgagc ttcggctgct tggcgacgcg cgcgagcagg aggagcacgc
    17101 ctttggagtc gagcacgtgg atcgagacga ccgccgagcc acgctggacc gtgcctacgc
    17161 cttcggcatc gagcggcggc aagctgaggt tgcgctcgcg gccgaatcga tcgaggtact
    17221 ccgccatccg cgccttgtgg tcgacgacct cttctccagc tgccatgcgc ggagcctact
    17281 acgaagactt cgtcggcttg tcgtcgtcgt cggaagtaac gatcgcgatc agctcggcat
    17341 caccgtcgag cggcaggctc ggtgtccact tcggcttgcg gatgcggacg acgcccgcgg
    17401 actgcggatg cggctcggag atatcggcag cgagctgcgc gagctcggct ttggcttcga
    17461 agctgctcgg gcgatcgaag cgatcccacg cgagcatctg atcgacgagt tggcagagct
    17521 gcggtggagc gtccgggcac cggacttcgg tggggacgtg ctgcgtgctg ccgtcggcga
    17581 cggttgcgat ccacacgccg tcgaacggca tgtcaccggt cagcgcttgg tacgcgatga
    17641 cgccgagcgc gaagatgtcc gcgcgatcgt cgacggcgtc gccgttgacg agctcgggcg
    17701 ccgtgtagtc ggcggggccg aataggcgcg cgggcgcgga cgtgtcgtgt gcacgtgcgt
    17761 cgctccagtc ggtgatgcag agcggaaacc cgcgcgtgcg gccggcaacg acgatgtttt
    17821 ctggcttgag cccgcagtgg acgacgccgc ggcgatgcgc gtgatcgagg atctcggcga
    17881 tgtcgcgaag catcgcggca gctgccgctg gatcgagcgg gccgttgagc agcatgtcgc
    17941 gaacggtcga gccatcgacg cgctcgtacg cgaaccacgc gcggcgctct gggatcagcc
    18001 ccgactcgta gacccgcaca ctgcctggat ggtcgagcgc ctcgaggatg cacgcctcgc
    18061 gaagcagctg caccgcgagt gcttgcggcg agacctcgtg catcaccttg acgaaggcac
    18121 ggcggggaag cacgagatgc cgcgcttcat agacgacact gtcggcgtct cgccttctct
    18181 ctcgatcgat gcggtagttc ccgatccgat ccacgacctc gggtttcata ccccgggccc
    18241 ccgtcggagc ctaggccttt ctgatataga ggggcgtgct tcgcagcgcg ctcgtgaccg
    18301 tagcggtggt cgtggctgta gcgctcgtcg cctgtcggcc ggaccacggt ccaccgcgcg
    18361 tgaagcggct tcctccgaag gaccaaacgc ctgcactgcg cggtggtggg acagcgaaat
    18421 cacctcgcat cgcgagttac aagatcgagg cgcgtctcga tgcttcgcgt cattcgctgc
    18481 aggcaacgca gacgctcatc tggacgaacg cgggtcagag cgcggtggac agactcccgt
    18541 tccacctcta catgaacgcg ttcaagagcg agtcgtcgct gttcatgcag agcacccacg
    18601 gcgagatgcg cggcgcgaaa tcgaccgaca gtggctgggg ctggatctcg atcgagagcg
    18661 tgcaaatcgc cggcaccgag ctcgcgaaca agctcgagta cccgctcgcc gcgcaaggcg
    18721 atgagaccgt cgcggagctg ccgctcgtca ccgcgatcga gcccgggcaa acgatcgaag
    18781 tcgcatttcg cttcagcgtg cagctgccgg aggtgttcgc gcgcaccggc tacaaaggcg
    18841 acttccacat cgtcgcgcaa tggttcccca agatcggcgt gcgcatcggg ccgccgggcg
    18901 cggaacaatg ggagtgccag ccgctgcagg cgacgaccga gttcttcgcc gacttcggca
    18961 actacgatgt gacgctcacc gtaccgaata cggaagtcgt cgcggcgacc ggcgtgctcg
    19021 tcggtgcgat cgatcagccc ggtcagatgc ggcagtacac gtatcacgcc gaggacgtgc
    19081 acgacttcgt gtggatggcc gacccgtaca tggaagtcca caagggcgag gccaagctcg
    19141 acgacggcaa ggtcgaggtc cgcgtctacg cgcgcaagga acaggcgatg tatgcgaagc
    19201 gccacctgca agcgggcatc ggcgcgatcg agaagttcag cgcgtacttc gtgccgtatc
    19261 cgtggccgat catgtcgatc atcgatcctc ccgtcgacgc gtggatgggc gcgggcggca
    19321 tggagtatcc gacgttcgtc acgaccgctg gcgacacggt gctcgcgcgt cctgggcttc
    19381 gcttgcccga gtacgtcacc gtgcacgaga tcgggcacaa ctggttccaa ggcatcctcg
    19441 cgtcgaacga agccgaggaa gcgtggctcg acgagggcat caacgagtgg gccgactcgc
    19501 acgtcatgcg cgatctgtac ggacctcgga cgagcgcgac cgactggatg ggctggcaag
    19561 cggaggtcga cagcctgcgc gctgcgatcg ccgacgatcc agaaggcatc ccatcgccga
    19621 tcgcgtctgc cgcgtacgcg ttcgtcgaca ccgacgccta cggcgaagcg acgtacacct
    19681 cgacgatgcg cgcactcgcg acactcgaga acctcgtcgg ctcgagcaag ttcatggccg
    19741 cgatgaaggt ctacgcaaag gagtttgcgt tcaagcatcc gacggcgcgc gacctctaca
    19801 cggtattgca gcgcgagctc ggcgaggacc tcgggtggtt cttccagccg gtgttccagc
    19861 aagtcggcgg cagcaagctc tcggtgcgca gcgcggactg ccggcagaac cacaaggttc
    19921 gcggcgtcga gggcgatggg cctggtcgca agacgtttac cgaaacggag agaccggatc
    19981 tcggcacgtt cgtctgcgat gtcgtcgtgc agaaccccgg catcatccac atccccgtcg
    20041 atgtcgagct gcgattcgcc gatggctcga gccagcgcgt cacgtgggat gatcgcggcg
    20101 gcagtcactg gaagcgctgg accgtcgagc gctcgagcaa gctcgtcgag gttcggatcg
    20161 atccagacca caagatcctg ctcgcgaacc cgacgacgaa tgcgatgcgc ctcgagggcg
    20221 acggctcggc atcgctgcgt gcctccgctc gcatcgcatc gtggtcgcag ctgctcatgc
    20281 agatcgtggg gccgtgatgg cgactcgcct cggtctcatc gatctgttcc gcgcggcgcc
    20341 gcgttcgatc ggtcgctaca ccggcacgct gctcgcggtg ttcgtcgtgc agacgatcgt
    20401 cacgctcgcc gtcatgatgg gcgtcgctat cgtgctcgca caagcgttcg gcaagctgcc
    20461 gatcttcgac gacgcggtcg acggcgatct cgtctcgctc atctggtgtc tgcgcttcgg
    20521 taaggcgaac ttcctcgccg tcatcggcat cgtgctcggc ggcgtgttgc tctggcagct
    20581 cgcatcgtgg ttcctcgtcg gcggtctcta cggcgtgctc gcgaatcggc ctgagggtcg
    20641 ccgcgaaacg gcgcgctgct tcggtgcgag cggcacgaca acctatctcg cgtacgcgcg
    20701 cctggccttg tgctcgattc ccggctggtt cgtcgtgctc tcggtattcg cgcaatcgct
    20761 gttgctcgtc gagaaacggc tcgaatacgc gctgacgatc ccgcagctcg ccggcgcgct
    20821 cgcgatcgca ttcttgccgg cgctgcttgt actgcacgtg ctgtggacga tcagcgacta
    20881 cgcgcgcgtc gagctgacac tgcgaggcga gtcgcacgag ccgagcgtcg tgatgacgta
    20941 cctgcgcacg gcgatgtacg tgctcaaacg tcccgcgaca ctcgcgcacg caggcatcgg
    21001 ttggctcgcg ttcgtcctcg tcacggtgat ctatgcgtac atcgcgcaag gccatccgat
    21061 gtacggcgcc gagggcgcga tcgcgctgtt cgtgatccgc gaaggtgtcg cgctcgcgcg
    21121 aaccgcgatt cgcttcggcg tgctcgctgg acagatcgag ctcggcaaga cgcgcgcgtt
    21181 gccaccgatg cacgtgaagg tcgacgcgaa gaagagcggc tagcgcgtcg agatatagag
    21241 gtcgccgtcg cgcgagaaca ccatcgtgcg ccgatcggcg gagatccacg gatcgctgcc
    21301 agtcgaatcg ccctcgaacg cgggatacgt gctcgtgaag aggaacgcat cggcgacgtt
    21361 cggtcgcgtg gtctcgtaga tctgacgcac gccggccatt cgctcggtgt gaaagaacat
    21421 gacgagcccg tcttgatcga gacaccccgc gccgtcctca ctcgtcgatg cgacttcgac
    21481 gacgtgacgc tccggtcccc agggatccat ttgggtctcg cggctcagca catacagatc
    21541 gttgtcggag gtccctaccc tggcgttgac gacgaactgc agaccatcgc cggagatcgc
    21601 tgggtttccg acgtcgatgg tcggcatgat cgtggtcttc gtgggattcg accaggtgcc
    21661 gcccgatggt cgcgtcgact tgaacacgcc gttgttgccc atgcgagtga agtagagctc
    21721 cgtcccgtcc gcattcactt gtcctgcttc gccggacgac gtcgacaact cggtgacgac
    21781 cgtcggtgct ccgaagctcg cagtcacgga tgcgcgcgtc gcgtgataga tgtcgaactg
    21841 gccgctcgcg tttccgcggt cgctggagaa gaagatctcg agaccgtcgc gggtcatgct
    21901 cgcgtcgctg tcgtacggat acttcgccga cgtgttcgga gcgttcaggc ctgtcagcgg
    21961 cgttggctgg ctccactcgg gcaacgcgcc atcgggtcgc acgatcatcg cgtcgggcgc
    22021 atcagcggtc acgcgaaggt ggtcgagctg gaacagcatg tcgcaacccg acagcagcag
    22081 cgcaagtgcg aagaaccgcg acacctcggc gaatggtatc gcgcccacgt acgagcagta
    22141 acgcgatacg atcttcgaat gaggaaccta acttgtgctc cgatgaccta cctgttgctt
    22201 ctcgttgcga gcaccgctgc gtgcggcggc gatgacgaga tcgattcgga cgagcaagcg
    22261 cgtcgcgcat acctgtgcct cgatggatcg atccagaagt cgatcgatct cgggtttgcc
    22321 ggcttcagcg cggcaagcag cgccaacatc cccgaacaaa cgggcgcggg cgacgtggcc
    22381 ggcacgatca aagtgaatgg ccaggtcgat cagggctcgt cgaacaacaa gggcatgcgc
    22441 ctcaacgtcg ggatggtcgg ctacgacgac ggcccggtcg agtacaaccc cgaccacgac
    22501 aaggtgcacg tcgtgttcga cacggccacg acggtcgcgg accagccgta tctctcgctg
    22561 cagctcaaga acattccgtc gggcacgctc gacggcacgc tgacgtcgaa cagctcgatg
    22621 catggcatct acacgctgtc gggggacatc cagggcacgc tcacgctgaa cctgacgatc
    22681 gccggcccca tcatggcggg ctcgggcaac gtcatcaccg tgcgcgttcc aggcatgacg
    22741 accgtgaccg gcacggcgac gaacagcgac ggcggcgttt acaacatctc catcacgatc
    22801 tagccgcggg cttgttgtat cgtgcggcgc atgtcgaatg cgcccacgct cgagagcctc
    22861 ctccaagaca gtggtttggc ggacaaggct caggttctcg aaccgaatct ggtccgcctc
    22921 cagtggggct ccgcgttcgt gatcgtcggg ctctccggca gcgcggtcgt cgcgatcgcg
    22981 ccgctgttcc gcgaaatccc gcgtgacaag gtcaccgcgt tctacaagaa gctgctcgag
    23041 cacaacgcgt acatgggcgg catggcgtcg ttcgcgattc agacggacgg ctgggtcatc
    23101 ctccacgccg gccgcgcgct caaaggcatc gacggacacg agtttgcgaa cctcgtcgcc
    23161 ggcgtcggcc gcttcgccga tcagttcgac gaccagctga tcaacgagtt ctacgtcgat
    23221 caccaagaag ctacgaatca ggctgacgtt cagtaattcg cacgtgcgac gggctgcgac
    23281 gcctgcgacg accgcgaacg aaacgcgcag cggcggcatt ctggttatga gtgcccgagc
    23341 tgaccgacgc ggatctggaa gcgctgaccg acgagcttgc gtgggttcgc cggctcgcgc
    23401 tgtcgctcgt tcgcgatcgc tctgacgccg acgagatcgc gcaggatacg tggctcgctg
    23461 cgaagcaaca cgcgccgcgt gatcggccgc tgcgcccgtg gctgtcacgc gtcgtcctca
    23521 acctggtgcg catgcgggcg cgcagcgatg cgcgtcgcgt aaagcgcgaa gcagcggtcg
    23581 tcgtcgagga ccacgcgccg agcccggcgg agctcgtcga ccgcgtcgag ctgcagcgaa
    23641 ccctcgtcga tgcggtgctc gccctcgcgg agccgtatcg cgcgacggtg ctgctccact
    23701 tcgtcgaagg gctcagctgc gcggagatcg cgcggcgttt tcagattccc gacggaaccg
    23761 ttcgtcgccg cctcaaggtc gcgctcgaca acctgcgcga gcagctagtg cccgacaaga
    23821 gccggtctcg gctctgggcc gtgctcacgc cgctcgtcgg ctctccctac gctgctggaa
    23881 ggatcgccat gaagaagctc gttgctgtaa tcgtagtcgt cgctgtcgtc gcgctcggga
    23941 tcgtgttcgt catcggtcgg gacaagccga agccgaaagc cggcgcggct ggcgacaagc
    24001 cggcgagcgg agctgtcgtg aacggcggca tgctcgatgg cgatgcgcag gtgaagccgt
    24061 ggttctccca gcgtggcgcg agcccacggc gcgtcgccgg aaaggtgctg ttcgagggcg
    24121 cgcccgtcgc aggtgcgatc gtgcggctcg cggatcacca cgagcttgcc gcgatcacga
    24181 cggcaaagga cggcgtgttc gatttcggca tgcagccgcc cgccgagctc tcggtgagtg
    24241 ccgaggctgc ggacaagaca ccggccgcat tgctggtgcc gctcgcagat ccgaacgtga
    24301 agagcgagaa catcatcctc gagctcaccg cgtgtaacgc gaagctgcac ggcagcgtcg
    24361 tcgacgcgtc gggtggcggt atcgcgaaag cgcatctgcg cgtggccgcg tttggcggtg
    24421 ctgaatcaaa cgctgccggc gagtactcgc tgtgcgtccc gatgggcgac tccgtcgtgc
    24481 gcatcgatgc agacggctac ggcggcctcg cgctgccgat ccatctcgtc ggtgcgcacc
    24541 accgggactt cgagctcgtg ccggaagcgg agctcgcggg caaggtcgtc gacgaagaag
    24601 ggtcagcggt tccgcacgcg cgcatcctcg ccgtgccgca ggcgatcgag acgccgcact
    24661 tcttgtcgag tggttacgcg atggcggatg ccgatggcgc attccgaatc tcgggcctcg
    24721 cacccggtcg cttcaacttg ctggcgaatg cagacggctt tagctcgacg agcccgaccg
    24781 cggccgtcgc actcgcaggc accggcaaca agagcgtcac gctcgtcgta gggatccgca
    24841 cgtcgatcag cgggcgcgtc gtcagcggtg acaagccggt ggcaggcgcg cgcatcacgc
    24901 tcgcgggtag cggcattcgc ttcgtgcggc cgagctactc gcagaaggac ggctcgttcg
    24961 tgctcgaagg cgttccggca ggtcgcgcgc ggctcgacgc aggaacgtac gaggtcgtgt
    25021 cgccgaaggc gctcgtcgtg ccgaagacgg gcatgaagga cgtcaagatc gaggtcgccg
    25081 agctcgcgac tctgcgcgga cacgtgacgc gcaaaggcga gccggtgtcg ggcgccatcg
    25141 tgcagacgaa tgtggggccg aaggcgcagg ccgatcaggc cggctactac gagatcaagg
    25201 gcctgcgtcc cggcacggta caagtgaccg cgcaggcgtt cggagcggtc aatgcgtttg
    25261 cgccgttcac gcccgtcaag ctcgcgggta acgcgccgac cgatcacgat atcgatctca
    25321 ccggtgccgc cgaagtgcgc ggggttgtcg tcgacgagag cggcgcgccg gtaccgaacg
    25381 tctacgtgcg cctcatcgaa gcccgcggcg atctcggcga gtcgatgacg gacgcgcagg
    25441 gccgtttcac gtgcacgtcg atgctcggca attcggatta ccgcgccgcg gtgtttccgt
    25501 cgccgggtgc gcgcgtcgca ttcccgccgg cgatcgacta cccggtcctg aagatcgccg
    25561 atggcaacac gatcctcgat ggcgtgaaga tcgcaatcaa gaatcagcgc ctgtcgatcg
    25621 cgggccacgt gatcgacgca gagggcaatc cggtctccga cgtccacgtc gaggcgatcg
    25681 gtcgaggctt cgcggccgga cctgcgatgt tgccgtcggt gcgtgcggat cagggaggcg
    25741 gcttcgtgat caaagatctg gcgccgggca cgtacacgct gcacgcgcac gcgggcgatg
    25801 gcagcgacgt ccagattccc gacgtcgcgg caggcgcggc gaacgtgcaa gtgcgcgtcg
    25861 aacggccagc gacgatcgaa ggcgacctcg tcggattcgc gtcgactccg cgcgtgcacg
    25921 cacgccagat gaccgcggac ctcgcgctca acaatgaagc gacgctcgag ggcaaccact
    25981 tcacgatcag tggcctcgca ccgggtcgct acgtcgtcga ggcgctcggc gagaacgaga
    26041 acgatggcga gaccgtcgag ctcaaggcag gcgccgtgct caaggtgaac ctcgagagcc
    26101 acggtcgcgg caccgtcgaa ggcatcgtga ccgagcttgg caccggcgcg ccggtcccga
    26161 acatgaactg cttcgccgcg ctgtcgatga acggactctc cggcgacatc ggccagaacg
    26221 cgccagtcaa cgacgacacg acgacgaacg cgcgcggcga gttcaagatc gcgtcgccgg
    26281 tcggcatggc gcgagtgatg tgcttctctg ccgacaactc gttcgccgtc gcgggcgcag
    26341 acatcaaggt cgcggcgtcg acaccgacga aggcgcagct cgtcgcggtg cgcgcgaatc
    26401 caccaccgag cgatccgggc ttccgtatcc gcccgatgac gctgccgctc gtgatcgcgg
    26461 cggtcgaccc aggcagccca gcggcgacag ccggtctcaa gatgggcgat gtggtgctct
    26521 cgatcgacgg cacgtcggtg aaggggctca tcccgagcgg cgcgatgatg ttggcctgga
    26581 accacaagcc ggggacgacc atcgcggtgg gcgtcgaccg gggcggcaag caggagacga
    26641 tttcgttcgc cgttcgagcg ccgacgaact aatcgcttag cagtgacatc agatctttcg
    26701 cggtgagccg cgcggcgacg tccgtacctt cgagcacgcc tgccacgagc gcgcgcttgt
    26761 cgcgatgcag cgcgacgatc ttgtcctcga tcgtgccgcg cgagatgagc cggtagaccg
    26821 tgacgggctt ggtctggccg atgcgatgtg cgcggtccgt cgcctgatcc tcgacggcgg
    26881 ggttccacca tggatcgaga tggatcacgt aatcggcggc cgtgaggttg atgccggtgc
    26941 ctccggcctt gagcgagatg aggaacgcgt cgccctcgcc tgcttggaag cgatcgatga
    27001 gcgacgcgcg atccttcgcg ggcgtcgagc catcgagata cagcgtggag aagccgtgtg
    27061 cgtcgagcgc ttcgcgaacg agcgcgaggt gcgaggtgaa ctgcgagaac acgagagcgc
    27121 ggtgaccttc gctgcgcagc tcgtcgagga gctcgatcgc acgcttgagc ttcgatgacg
    27181 cgatcgtcga cgcagagtcg tagagcttgg gatggctcgc gagcaggcga agacgcgtga
    27241 gagccgcgag cacttggaag cggcgttgct cgtcacgcat gccgccgcct ttaatcgtga
    27301 gctcggcgac tgcggccagg cgcgcatcct cgtagagctg gtgctcgtcg tcggagagcg
    27361 cgatcggcac ctcgacctcc gtgcgagatg gcagctcgcg agcgacctcc gccttcgtgc
    27421 gacggagcaa gaacggctgg atcacgcgcg caagcgccgc gcgcgcttcg gggtctttca
    27481 ccttttcgat cggcgccgca aaccgctcgc ggaactggtc ccagctgccg agtaggcccg
    27541 ggaacacgat cgagaacaga ctccacagct cgccgagatg attctcgagt ggcgtgccgg
    27601 tgagcgcgat ccggaagtcc gcgtcgagcg cgcgcgctgc cttcgctcgc tgcgtggtcg
    27661 gattctttag cgcctgcgct tcgtcgacga cgagcgtcgc gaacttggcc tgcgcgagct
    27721 gctgcg
//
LOCUS       DATLBZ010000313        12446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1101191, whole genome shotgun sequence.
ACCESSION   DATLBZ010000313 DATLBZ010000000
VERSION     DATLBZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101191"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..814)
                     /locus_tag="VMZ53_16175"
     CDS             complement(344..814)
                     /locus_tag="VMZ53_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30045.1"
                     /translation="MKQGRGPGAKKDDKDVPQPAVAPAQGKKTLVEAATPTKPATPTT
                     ANVAPATPNLTPTTANPTSTSTKKRADDDWQPNWRRLKSFAKRARVGDEDKTDEDAQA
                     EAAAEKAAADAQKTAAEAKAAADADRTAADGDVTHDEDSKPESDLLETTNKRDD"
     gene            complement(830..5215)
                     /locus_tag="VMZ53_16180"
     CDS             complement(830..5215)
                     /locus_tag="VMZ53_16180"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HUS30046.1"
                     /translation="MERQRAPQVQGDGQEARAPAAPAPGKTTLVQAMGGAHARGSASE
                     IGQRTDAAPPDQLGEDASKPRWAQLKSISKYKNAPESEATEGEELGAGAPLPDEQKGK
                     FEKSLGRDLSGVRVHDGPAAEAKADKVGARAYAQGQDIVFNKGEYAPGDKKKDGLLAH
                     EVAHTVQQRGASSGGASKGSAAPATTTPGDSVERGADSAAGAMVQGAPASVEAAPESI
                     ARKTKGEADPEPQEAAAAPGPLAAGSPAPSAAEPAGKADPAKDAVGKEGGGGDFSVQL
                     VGTNASEKPDVAGAVPGHNLEAGKAELAGIGGKLPASGAEGVGADGIKIDHPPAPAQA
                     PGGDAPVPKELADQIKQAQGETREAVTEAEGESAAYKAEIKAKRDNFEDEQHATMLES
                     VKSMSSTDKRSTLKDMGYDEKALKKIKDSELDGIISGKMETEQRKNKIMGMDPEELKA
                     LSPAQKIQYLVDLGIDRGDLEKAGQAKACKLFDDVMAVAHVPGEHTCKIKISGGFLGV
                     GKKDWVVNVKVDLDGNATMDVKKEGGWLSKLWGWVKAALPIILVCLAPLTGGASLIVL
                     AVYQAAMAIKNGDWLGAIVGAATALVGLGALTAAGAFAKVADVAKKVKDVATAAQASL
                     NAAKAKNAGSLLAALAGGAGAFAGFAANSADKFAKTMQGWSDKLKKWSSIVSGGEKVV
                     KGIKSGDPIGAISGAFDTAAGVVGPTGAGAKNIARAQALTGFVNSGKRALSSNPPDYG
                     AVASAAMGIANTLKQDRRLEDAGRIVNAASRLEQAWKNKDANPTGLADAALDLASAIQ
                     VAKYDSDHPAEKDADGKDKPDQGREAVLDRYTQAQRVVRAAGSAIQAVMTKPRPNYIA
                     ALDAGSQLIAELTSDKRVDAAAIVTARLNAYTQAVNSKNEQSILEAAMSFGEAVDGLR
                     DSIGEARAKAKKEAEAQLQPGDKLPEGEAGEMPRFQTSTEVTAQAQVVPTIPPKIITE
                     LDPSELPGGTHGPQAPPAHAPQADGPQQGPQQGPQQGPQQGPVIGPAGPQSPETKPPG
                     AQPAKPPIPDDAKHIMDVIKIAADVAKLNGFTLGKALVDQWEATKKAFLDAHEDPKTT
                     AEVYSEKLKALKFIKSSGKFIDLVSKELSKYEGQFGGDNSDFKGAAMAFGRARRNFER
                     FVRVAEIPLTVLEAADNFIIVAGYKADQSGQPATMLERMQAASKLAVEATQLPGELAW
                     LGRIGARIAGKLGAQSIVAAANTVRTAINRFGDDAARVVMKRIMPILERAATVETPAI
                     ITRLGESWAGEIASWGATQLARAASNDLIKGAFAQLATRFGFAARITVSAPAAIAFEV
                     AELEAHLAAEMYKDAYKSISHFLSARAFGKTIDQWKNDIRSQSFGTPEAADAFFNWAF
                     YKFLNSASTEVRGGWLPYTWSALPSRAIAGSQLANFSGTRNPVEILTKLEPGDLKTMI
                     VGPALQEVALAYLDREYQRIFHEAP"
     gene            complement(5290..5859)
                     /locus_tag="VMZ53_16185"
     CDS             complement(5290..5859)
                     /locus_tag="VMZ53_16185"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HUS30047.1"
                     /translation="MSRGPALGVLEVGTIARGVVTADAGLKRSPAILLHSRAVSGGKH
                     LVFFEGGVAEVEEAMSAAKRIAGDLLLDRVELSAADDQVWPMLGAPLTPVDWSTDPDA
                     EAVAIIETKTVCAAIAAADAACKVAAVLVRDTRFAVDLAGKAYFTLTGSLAAIEAAAI
                     AATDAAADRLVGLEVIAQPAPELRGKLFR"
     gene            complement(5856..7316)
                     /locus_tag="VMZ53_16190"
     CDS             complement(5856..7316)
                     /locus_tag="VMZ53_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUS30048.1"
                     /translation="MAYVGGLDERKIEEIVSRVLDRLGGGGVTAAAPVRGGAPEAAKV
                     KSNVPRGTNGVYQDADQAAKAARKAFEQNERTPIATRVKMVENMRKAVLSNVDALSRY
                     AVEETGLGRYEDKLSKNTLVANKTPGVEILRAQAYTGDDGLMLTERAPYGVMLAITPT
                     TNPTETILNNAIGMVAAGNAVVFNVHPSAAQTCNWCVHLLNEAIQAAGGPRDVIVSVE
                     KPTIESANTLMKHPLVRIVVVTGGPAVVKQAMNSGKKAICAGPGNPPAVVDETANLEK
                     AADAIVRGASLDNNIVCIAEKEVIAVDSIANELINQLKRKPVLWLDNRHVKELEKVVL
                     EGDHVNKNWVGKDAGVIAKAIGIGGHGHDLRLLMMEVDEAHPFVQHELLMPVIPLFRV
                     RDSADAIAAALRVEHGFHHTATMHSTNIDNMSAMARLCNCSIFVKNDASYAGLALGGE
                     GYTSFTIASPTGEGLTTAVHFTRERRCTLKEAFRFV"
     gene            complement(7319..7609)
                     /locus_tag="VMZ53_16195"
     CDS             complement(7319..7609)
                     /locus_tag="VMZ53_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HUS30049.1"
                     /translation="MVLGKVIGTLVASRKEPTLEGLKLLVVRACDVDGTPTGAIAVAV
                     DAVGAGLGEVVLYCAGSSARQTQVTQNRPVDATIMAIVDEVAVDGKSRYVKD"
     gene            complement(7616..7924)
                     /locus_tag="VMZ53_16200"
     CDS             complement(7616..7924)
                     /locus_tag="VMZ53_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HUS30050.1"
                     /translation="MADALGMIEVKGFVGMVEAADAMVKAAKVELVGYEKTGGGYVTA
                     VVRGDVAAVKAATEAGQRAAERVGEVVSVHVIPRPHANVDAVLPLGRAGNGGGGGNTK
                     "
     gene            complement(7946..8587)
                     /locus_tag="VMZ53_16205"
     CDS             complement(7946..8587)
                     /locus_tag="VMZ53_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30051.1"
                     /translation="MAATVLRTYCFLDALQPQQATFIGKTARGFLPVPYQASLWVEIA
                     PGIAINQVTDAALKATNVQPAVQVVERAYGLLEVHANDKGEVLQAGATILKHLGVEEN
                     QRLKPRVLTNQIIRGVEAYQTQIINRSSAGMMVLPGQSLFIFECEPAGYAVLAANEAE
                     KAAHVYLVNVTPYGAFGRLYMAGPEAEIDAAAAAATAALNAVTGKEPEKFVDR"
     gene            8697..10244
                     /locus_tag="VMZ53_16210"
     CDS             8697..10244
                     /locus_tag="VMZ53_16210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUS30052.1"
                     /translation="MMRAPMRRWPLIVCSLVALTHVAHADLTSRALSAADEAGGSSAQ
                     NSLLTWAGTPKQTSLPDLLQVAVRSSPALQNAKLDIEIAEAQIQQTWARRDWVLRAQA
                     SGSWTQSGLVAGVTVGSSKRFAATLDISRMFPTGGTIGLHAGSSYSKTTSTFGPSEYW
                     SDDISLEITQPLLRGRGRSLYDANEARARIARDAAVLSRRLAALETIQTIVSAYWDLV
                     LAQRQIAITESSLALARERLRITEIGANGGKIAPAEIPAVQQIIATREEDVLGGELAV
                     LDRSIALRRSVNMPIDAGDIGLRVATELETQDKGWTLASLLQKAYAASPELATLAKQE
                     SGSTIEIEVTENGLLPQLDLALSFGPTGTDEKFGTAAANTFKFSELAASGSLTLTQSL
                     HRDDVRGRVKELRLGREKLRVNALDIRAQIAQTLTRAVAQIELAKRRVVLSQKAIDLA
                     NENIRIETDRFNLGKATNFDVLNRQEELRQAELRKAQALIDWHKAETVVMTVTGDLLP
                     AFGITVE"
     gene            10365..11723
                     /locus_tag="VMZ53_16215"
     CDS             10365..11723
                     /locus_tag="VMZ53_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30053.1"
                     /translation="MRRYSPLLLTLLPLAACGSDEPPSPTDVRTAIHDDLRHILTEGQ
                     SAAQKSTVNLPTASMLSIISPDLVLGDPMAAIDNMEADELTTKLENELFTDANYLGNG
                     IYKVPPSLVCKETIYNDDGTTTEQLDPDCAQQLDKIQLRIRVATEDDAVRFFVQVDAN
                     HDEPLSLLLAHDRLAVTVNLDDATAAMIALSAIEGEPPPNADLSGSVTGSLTILGTAH
                     AQVALSFDRDISIKFADAGVALDSDAATRFTSVAAHVIAVELDGNAPKAKFDLGLGET
                     TAHIAPDIDSVDPRSEDFLLAGATVNATYQANALTLDNISLGSKTSQLSIGGQVAQTI
                     DLNATAGRKLAAVITSNPATGEETLEVTPRLDLQMTTDHAVLADEAPRYDVTRILLDG
                     SLRGMADSSQLEVVSGSFSMTTNPSQYGFSATAGQCVTSTSEYDPVTYEDYELYSVGA
                     CL"
     gene            11764..12426
                     /locus_tag="VMZ53_16220"
     CDS             11764..12426
                     /locus_tag="VMZ53_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30054.1"
                     /translation="MRYASLVLVCGCGRLSFEPLADAPERAADAPADAPTPPCLVEDF
                     STDTGMWQPVLGSYSLQPGAGPDGSPAYVGAIQPENLATNAALRGIRTARVRLDFRID
                     TANGDFNVFWFKPFTSYATATGYEALLVPANSDTLNDALGVWTNGSVQTFAQRPGTIV
                     MSTWHHIDAELRADGSMQLDLDGAPWLSSPPDATVSPPFDVILRLFYQGAIDNISVDC
                     TR"
BASE COUNT         1876 a         4310 c         3931 g         2329 t
ORIGIN      
        1 ctgcaggatg cgctgcgcga tatcagcctc gacggcgatc gctagtcgcg cgtccgtgcc
       61 gcgcctgtgc cgcgggtcgt tcgcacgggc aggacgcgtg cccgttcgca cgggcaggac
      121 gtgtgccccg gatttaacgc agaggacgca gaggcacaga ggacgcggag aaagggtttc
      181 gggttcgcac gggcaggacg tgtgcgccag gtcattcgca cgcgttcgca gggcaggacg
      241 tgtgcgccgg gtcgttcgca cgcgttcgca tgggcacgac gtgttccgag tcgttctctc
      301 ggaggacgtg cccagcgatt tgcgtggtcc gggacgtcgg agcttagtcg tcgcgtttgt
      361 tcgtcgtctc gagcaggtcg gactcaggct tcgagtcctc gtcgtgcgtc acgtccccat
      421 cggccgccgt cctgtcggcg tccgctgcgg ccttcgcttc ggccgctgtt ttctgggcgt
      481 cggccgctgc cttctcggcg gcggcttcgg cttgcgcgtc ctcgtcggtc ttgtcctcgt
      541 cgcccacgcg tgcgcgcttc gcaaatgact tcaggcgccg ccagttcggc tgccagtcgt
      601 cgtccgcgcg cttcttcgtc gatgtggatg tcgggttcgc ggtcgtcggc gtgaggttcg
      661 gcgtggccgg ggcgacgttg gccgttgttg gggtggccgg ctttgtcggc gtggcggcct
      721 cgacgagcgt ctttttgcct tgagcgggcg cgacggccgg ctgcgggacg tctttgtcgt
      781 ccttcttcgc gccagggccg cgtccctgct tcatatgggg agtgtagcgt tacggcgctt
      841 cgtggaagat gcgttggtac tcgcgatcga ggtatgcgag cgcgacttct tggagggccg
      901 gacccacgat catcgtcttg aggtcgccgg gctcgagctt tgtcaggatc tcgacggggt
      961 tgcgcgtgcc gctgaagttt gcgagctgcg agccggcgat cgcgcgcgac ggcagcgcgg
     1021 accacgtgta cggcagccag ccgccgcgga cttccgtgct cgcgctgttg aggaacttgt
     1081 agaacgccca gttgaagaat gcgtcggcgg cttcgggcgt cccgaacgac tgcgagcgga
     1141 tgtcgttctt ccattggtcg atcgtcttgc cgaaggcgcg cgcgctgagg aagtgcgaga
     1201 tggatttgta ggcgtctttg tacatctcgg cggcgagatg cgcttcgagc tcggcgactt
     1261 cgaatgcgat cgctgcgggc gcgctgaccg tgatgcgagc ggcgaagccg aagcgcgtcg
     1321 cgagctgagc gaatgcgccc ttgatgagat cgttcgaggc tgcgcgcgcg agctgcgtgg
     1381 cgccccagct cgcgatctcg ccggcccatg actcgccgag gcgcgtgatg atcgcgggtg
     1441 tctcgacggt ggccgcgcgc tcgaggatcg gcatgatgcg cttcatcacg acgcgggcgg
     1501 catcgtcgcc gaagcggttg atcgcggtgc gcacggtgtt ggcggccgcg acgatcgatt
     1561 gggcgccgag cttgcccgcg atccgcgcgc cgatgcggcc gagccatgcg agctcgccgg
     1621 gaagctgcgt cgcctcgacc gcgagcttcg acgccgcctg catgcgctcg agcatcgtcg
     1681 ctggctggcc cgattgatcg gctttgtagc cggcgacgat gatgaagttg tccgccgctt
     1741 cgagcacggt cagtgggatc tcggcgacgc ggacgaagcg ctcgaagttg cggcgtgcgc
     1801 ggccgaacgc catcgcggcc cctttgaagt cgctgttgtc gccgccgaac tggccttcgt
     1861 acttgctgag ctccttgctg acgagatcga tgaacttgcc gctgctcttg atgaacttga
     1921 gggccttgag cttctcgctg tacacctcgg cggtcgtctt tggatcttcg tgcgcgtcga
     1981 ggaatgcctt cttcgtcgct tcccattgat cgacgagcgc cttgccgagc gtgaagccat
     2041 tgagcttcgc gacgtcggcg gcaatcttga tgacgtccat gatgtgcttg gcgtcgtcgg
     2101 gaatcggcgg ctttgcgggc tgcgcgccag gcggcttcgt ctcgggagac tgcggacccg
     2161 ctggaccgat gaccggccct tgttgcggac cctgctgcgg gccttgctgc gggccctgct
     2221 gcggaccatc ggcttgtggc gcgtgcgccg gcggagcctg cggtccgtgc gttccgcccg
     2281 gcagctcact cggatcgagc tcggtgatga tcttcggcgg gatcgtcggc acgacctgcg
     2341 cctgcgcggt gacctccgtc gacgtctgga agcgcggcat ctcgccggct tctccctcgg
     2401 ggagcttgtc gcccggctgc agctgcgctt cggcttcctt cttcgccttc gcgcgcgcct
     2461 cgccgatcga atcgcggagg ccgtcgacgg cttcgccgaa cgacatcgcc gcctcgagga
     2521 tcgactgctc gttcttgctg ttgaccgcct gcgtgtacgc gttcagtcgc gccgtcacga
     2581 tcgccgctgc gtcgacgcgc ttgtcactcg tgagctcggc gatgagctga gagcccgcgt
     2641 cgagcgccgc gatgtaattg ggccgcggct tcgtcatcac cgcttggatc gccgagcctg
     2701 cggcacgcac gacacgctgc gcttgcgtgt agcgatcgag gaccgcttcg cgaccctgat
     2761 cgggcttgtc cttgccgtcg gcgtccttct cggctggatg atccgagtcg tacttcgcga
     2821 cctggatcgc ggacgcgaga tcgagcgccg cgtcggcgag gcccgtcggg ttcgcgtcct
     2881 tgttcttcca cgcctgctcg aggcgcgacg ctgcgttgac gatgcgaccg gcatcctcga
     2941 ggcgacgatc ttgcttcagc gtgttcgcga tacccatcgc ggccgatgcc accgcaccgt
     3001 agtcgggtgg attgctcgac agcgcgcgct tgccggaatt cacgaagccc gtcagcgcct
     3061 gagcgcgcgc gatgttcttc gcaccggcgc ccgttggacc gacgacgccc gctgccgtgt
     3121 cgaacgctcc gctgatcgcg ccgatcggat cgcccgactt gatgcctttg acgaccttct
     3181 cgccgccgct gacgatcgag gaccacttct tcagcttgtc cgaccagccc tgcatcgtct
     3241 tcgcgaactt gtcggcggag tttgcagcga agcccgcgaa cgcgcctgca ccgccggcga
     3301 gcgccgcgag cagcgagcct gcgttcttcg ccttcgcagc gttgagcgat gcctgcgccg
     3361 cggtcgcgac gtccttcacc ttctttgcaa cgtcggcgac cttcgcgaat gcgccggccg
     3421 cagtcagcgc gccgagaccg acaagggccg ttgcggcacc gacgatcgcg ccgagccaat
     3481 cgccgttctt gatcgccatc gcggcctgat agaccgcgag cacgatcagc gacgccccgc
     3541 cggtcagcgg tgcgagacag acgaggatga tcggcagcgc tgctttgacc cagccccaca
     3601 gcttggagag ccatccgccc tccttcttga cgtccatcgt cgcgttgccg tcgagatcga
     3661 ctttgacgtt gacgacccag tctttcttgc cgacgccgag gaagccgccg ctgatcttga
     3721 tcttgcacgt gtgctcgccg gggacgtgcg cgaccgccat cacgtcgtcg aacagcttgc
     3781 acgccttcgc ctgtcccgcc ttttcgagat cgccgcggtc gatgccgaga tcgacgaggt
     3841 actggatctt ctgcgccgga gagagagcct tcagctcctc cggatccatc cccatgatct
     3901 tgttcttgcg ctgctcggtc tccatcttcc cggagatgat tccgtcgagc tcgctgtcct
     3961 tgatcttctt gagggccttc tcgtcgtagc ccatgtcctt gagcgtcgac cgcttgtccg
     4021 tcgagctcat cgacttcacg gactcgagca tcgttgcgtg ctgctcgtcc tcgaagttgt
     4081 cccgcttcgc cttgatctcg gccttgtacg cggcgctctc gccttcggct tcggtgactg
     4141 cttcgcgcgt ctcgccttgc gcctgcttga tctgatcggc gagctctttg gggactggcg
     4201 cgtcgccacc gggtgcctgc gctggtgcag gcgggtgatc gatcttgatg ccgtcggcgc
     4261 caacgccttc tgcgccactc gcgggaagct tgccgccgat acccgcgagc tctgccttgc
     4321 ccgcctcgag gttgtgcccg ggcaccgcac ccgcgacgtc tggcttctcg ctcgcgttcg
     4381 tgccgactag ctggacggag aaatcgccac cgccgccttc ttttccgacg gcgtccttcg
     4441 ccggatccgc tttccccgcg ggctcggccg cgctcggagc gggcgatccg gctgcgagcg
     4501 gaccaggagc ggcggcggcc tcttgcggct cgggatccgc ctcgcccttc gtcttgcgcg
     4561 cgatcgattc gggcgctgcc tcgacacttg ccggcgcacc ttgcaccatc gcgccggcgg
     4621 ctgaatcagc gccgcgctcg acggagtcgc ccggtgtcgt cgtcgccggc gctgccgaac
     4681 ccttcgatgc accgcccgac gaagcaccgc gctgctgcac cgtgtgcgcg acctcgtgcg
     4741 cgagcagtcc atctttcttc ttgtcgccgg gtgcgtactc acccttgttg aagacgatgt
     4801 cttggccttg cgcgtacgca cgcgcgccga ccttgtcggc cttcgcttcc gcagcagggc
     4861 cgtcgtgcac gcgcacgccc gacagatcgc gaccgagcga cttctcgaac ttgcccttct
     4921 gttcgtcggg cagcggcgcg cccgcgccga gctcctcgcc ctcggtcgct tcgctctctg
     4981 gcgcgttctt gtacttcgag atcgacttga gctgcgccca tcgcggcttg ctcgcgtcct
     5041 cgccgagctg atcgggtggc gcagcatccg tgcgctgtcc gatctcgctc gcgctgcctc
     5101 tcgcgtgcgc cccgcccatc gcttgaacga gcgtggtctt gccaggagcg ggcgcagctg
     5161 gcgcacgggc ctcttggcca tcaccctgaa cctgcggcgc gcgttgccgc tccacgtctc
     5221 gattctacgc gtgatgcaga cgtgaggttc ccaacgcacg taacagctgc gtgtcggaca
     5281 acgtgcgcgt cagcgaaata acttcccgcg gagctcggga gcgggctgag cgatcacctc
     5341 gagaccgacg agtcgatcgg ccgctgcgtc ggtcgcggca atcgctgcag cctcgatcgc
     5401 agcgagcgat cccgtcagcg tgaagtaggc tttgcccgcg agatcgacgg caaatctcgt
     5461 gtcgcgaacg agcaccgccg cgaccttgca cgccgcatcg gcagcggcga tcgccgcaca
     5521 cacggtcttg gtctcgatga tcgcgacggc ctcggcatcc ggatcggtcg accaatcgac
     5581 gggtgtcaga ggagcaccga gcatcggcca cacttggtcg tcggccgccg agagctcgac
     5641 gcgatcgagg agcaggtcgc cggcgatccg cttcgcggcg ctcatcgcct cttcgacttc
     5701 ggcgacgccg ccttcgaaga acacgagatg cttgccgcca gagacggcgc gcgagtgaag
     5761 caagatcgcg ggcgagcgct tcaggcccgc atccgctgtg acgacaccgc gcgcgatcgt
     5821 gccgacctcg agaacaccga gtgccggacc gcgactcaca cgaacctgaa cgcctctttc
     5881 agcgtgcagc gtcgctcgcg cgtgaagtgc acggcggtgg tcaacccctc gccggtcggc
     5941 gacgcgatcg tgaacgacgt gtacccctcg ccgccgagcg ccagtcccgc gtagctcgca
     6001 tcgttcttca cgaagatgga gcaattgcag aggcgcgcca tcgcactcat gttgtcgatg
     6061 ttcgtcgagt gcatggtcgc ggtgtggtgg aagccatgct cgacgcgcag cgccgctgcg
     6121 atcgcatctg ccgagtcgcg cacgcggaac agcgggatga ccggcatcag cagctcgtgc
     6181 tgcacgaatg ggtgagcttc gtcgacctcc atcatgagga ggcgcaggtc gtggccatgg
     6241 ccgccgatgc cgatcgcctt tgcgatgacg cctgcgtctt tgccgaccca gttcttgttc
     6301 acgtgatcgc cttcgaggac gaccttctcg agctctttga cgtggcggtt atcgagccag
     6361 aggaccggct tgcgcttgag ctggttgatc agctcgttcg cgatcgaatc gaccgcgatg
     6421 acttctttct cggcgatgca gacgatgttg ttgtcgagcg aagcgccgcg cacgatcgca
     6481 tcggcggcct tctcgagatt cgcggtctcg tcgacgacgg cgggcggatt accaggcccc
     6541 gcgcagatcg ctttcttgcc cgagttcatc gcctgcttga cgacagccgg tccgcccgtc
     6601 acgacgacga tgcgaacgag cggatgcttc atgagcgtgt tcgcgctctc gatggtcggc
     6661 ttctcgacgg agacgatcac atcgcgcggg ccgcccgcgg cttggatcgc ctcgttgagc
     6721 agatgcacgc accagttgca cgtctgcgcc gccgacggat gaacgttgaa cacgacggcg
     6781 ttgcccgccg cgaccatgcc gatcgcattg ttcaaaatcg tctcggtcgg attcgtcgtc
     6841 ggtgtgatcg cgagcatcac gccgtagggc gcgcgctccg tcagcatcag gccgtcgtcg
     6901 ccggtgtacg cctgcgcccg caggatctcg acgccgggcg tcttgttcgc gacgagcgtg
     6961 ttcttcgaca gcttgtcctc gtagcgacca agcccggtct cttcgaccgc gtagcgcgac
     7021 agcgcgtcga cgttcgacag caccgccttg cgcatgttct ccaccatctt cacgcgcgtc
     7081 gcgatcggcg tgcgctcgtt ctgctcgaac gccttgcgcg ccgccttcgc cgcctgatcg
     7141 gcatcttgat agacaccgtt cgtcccgcgc ggaacattcg acttcacctt cgctgcctcc
     7201 ggtgcgcccc cacgcaccgg agcggcagct gtcacgcccc cgccacccaa acgatctaaa
     7261 actctcgaga cgatctcttc gatcttgcgt tcgtcgagtc cgcctacgta cgccatcgct
     7321 agtccttcac gtagcgagac ttgccgtcga ccgcgacctc atcgacgatc gccatgatcg
     7381 tcgcgtcgac gggacggttc tgcgtcacct gcgtctgccg cgccgagctt ccggcgcagt
     7441 agagcacgac ctcgccgagg ccagcaccca ccgcatcgac cgcgactgcg atcgcgccgg
     7501 tcggagtgcc gtcgacgtcg caagcccgca cgacgaggag cttgagcccc tcgagcgttg
     7561 gttccttgcg gctcgcgacg agcgtgccga tgaccttgcc gagcaccatg cggagctact
     7621 tcgtgttgcc gccgccgccg ccgttgccag cgcgtccgag cggaagcact gcatcgacgt
     7681 tcgcgtgcgg gcgcggaatc acgtggaccg agaccacctc gccgacgcgc tctgcagcgc
     7741 gctggccggc ctcggttgcc gccttcacag cagcgacgtc accgcgcacg acagccgtga
     7801 cgtaaccgcc gcccgtcttc tcgtaaccaa cgagctcgac cttggcagct ttgaccatgg
     7861 catcggcggc ttcgaccatt ccgacgaagc ctttgacttc gatcattccg agggcatcag
     7921 ccatttctgt tctccattac cttcgctagc gatccacgaa cttttcgggt tctttgccgg
     7981 tgaccgcgtt gagcgctgcg gtcgcggcgg cagccgctgc atcgatctca gcttcgggac
     8041 ccgccatata gaggcggccg aatgcgccgt acggcgtgac attgacgaga tacacgtgcg
     8101 cggccttctc ggcctcgttc gcagcaagca ccgcgtagcc ggcgggctcg cactcgaaga
     8161 tgaagagcga ctgaccaggc aacaccatca tgcccgccga gctgcggttg atgatctgcg
     8221 tttggtacgc ctcgacgccg cggatgatct ggttcgtcag gacgcgcggc ttgaggcgct
     8281 ggttctcttc gacgccgagg tgcttcaaga tcgtcgcgcc ggcctgaagg acttcgcctt
     8341 tgtcgttcgc gtggacctcg agtagtccgt atgcgcgctc gacgacctgc acggctggct
     8401 gcacgttcgt ggccttcagc gccgcgtcgg tgacttggtt gatcgcgata ccgggcgcga
     8461 tctcgaccca cagcgaggcc tgatacggaa ccggaaggaa tccacgcgcg gtcttgccga
     8521 tgaacgtcgc ctgctgcggc tggagcgcgt cgaggaaaca gtacgtacga agtacggtgg
     8581 ctgccatccc agttggcgca cgctagcacg gtgcccgaac ggaattccat gcgtggactg
     8641 ccaaacgtcg cagtcgtcgc atgcgtcgca cggtgcgcgt cgtttttgtg catgccatga
     8701 tgcgcgcgcc tatgcgccgg tggccgctga tcgtctgttc gctcgtcgca ttgacccacg
     8761 tggcgcacgc cgatttgacg tcacgcgcgc tctctgcggc cgatgaggcg ggtggctcca
     8821 gcgcgcagaa cagcctcctg acgtgggccg ggacaccgaa acaaacgtcg ctgccggacc
     8881 tcctccaggt cgccgtcagg tcctcgcccg cgctgcagaa cgcgaagctc gatatcgaga
     8941 ttgccgaggc gcaaatccag cagacgtggg cgcggcgcga ctgggtcctg cgcgcgcagg
     9001 ccagcggctc gtggacgcag agcggcctcg tcgccggcgt gacggtcggc agcagcaagc
     9061 ggttcgcggc cacgctcgat atctcgcgca tgttcccgac cggcggcacg atcggcctgc
     9121 acgcgggaag ctcgtactcg aagacgacga gcacgttcgg tccgtccgaa tattggagcg
     9181 acgacatttc cctcgagatc acgcagccgc tgctccgtgg ccgtggtcgc tccctctatg
     9241 atgcgaacga ggcgcgcgcg cggatcgctc gtgatgccgc cgtgctgtcg cgccggctcg
     9301 ccgcgctcga gacgatccag acgatcgtca gcgcgtactg ggacctcgtc ctcgcgcagc
     9361 gccagatcgc gatcaccgaa tcgagcctcg cgctcgcgcg tgaacgcctg cgcatcaccg
     9421 aaatcggcgc aaacggcggc aagatcgcac cggccgagat tcccgccgtg cagcagatca
     9481 tcgcgacgcg cgaggaagat gtcctcggcg gcgagctggc cgtcctcgac cgatcgatcg
     9541 cgcttcgccg ctcggtgaac atgccgatcg atgcgggtga catcgggctg cgcgtcgcca
     9601 ccgagctcga gacgcaagac aagggctgga cgctcgcaag cctcttgcag aaggcgtacg
     9661 ccgcgagccc cgagctcgcc accctcgcga agcaggagtc cggatcgacg atcgagatcg
     9721 aggtcaccga gaacggcctc ttgccgcagc tcgacctcgc gctctcgttc ggcccgacgg
     9781 gcaccgacga gaagttcggc accgccgccg caaacacgtt caagttctcc gagctcgcag
     9841 cgagcggctc gctcacgctg acgcaatcac tgcatcgcga cgacgtgcgc ggccgcgtca
     9901 aagagcttcg gctcggccgc gagaagctcc gcgtcaacgc gctcgatatc cgcgcccaga
     9961 tcgcgcagac gctgacgcgc gcggttgctc agatcgagct cgcgaagcgc cgcgtcgtgc
    10021 tctcgcaaaa ggcgatcgat ctcgcgaacg agaacatccg catcgagacc gaccgcttca
    10081 acctcggcaa ggcgacgaac ttcgacgttt tgaaccgcca ggaagagctg cgtcaggccg
    10141 agctgcgcaa ggcgcaggcc ctcatcgact ggcacaaagc ggaaaccgtc gtgatgacgg
    10201 tgacgggcga tctgctcccg gcattcggca tcactgtcga gtgacgtagg tagttccggc
    10261 agtcattcga cacatcgtga acgatcgggc acagcgcacc gcgccaagtg cgcgattgcc
    10321 agcgcgggag cacatggcac cgccattgaa ctacctgctg ccccatgcgc cgctattcgc
    10381 ctctactcct cactctcctc cccctcgccg cctgtggttc cgacgaacca ccctccccga
    10441 ccgacgttcg gaccgcgatc cacgatgacc tccgccacat cctgacggag ggccaaagtg
    10501 ccgcgcagaa atcgacggtg aatctgccga cggcgtcgat gctgtcgatc atctcgccgg
    10561 atctcgtgct cggcgacccg atggccgcga tcgacaacat ggaggccgac gagctgacga
    10621 ccaagctcga gaacgagctg ttcaccgacg ccaactatct cggcaacggt atctacaagg
    10681 tgccgccgag cctcgtctgc aaagagacga tctacaacga cgacggcacg acgaccgagc
    10741 agctcgatcc ggactgcgcg caacagctcg acaagatcca gctgcgcatc cgcgtcgcga
    10801 ccgaagacga cgccgtgcgc ttcttcgtcc aagtcgacgc aaaccacgac gagcctctct
    10861 cgctcctcct cgcgcacgac cgcctcgccg tcaccgtcaa cctcgacgac gcgaccgcag
    10921 cgatgatcgc gctctcggcg atcgaaggcg agccgccgcc caacgccgac ctctccggct
    10981 cggtcaccgg ctcgctaacc atcctcggca ccgcccacgc gcaggtcgcg ctgtccttcg
    11041 atcgcgacat ctcgatcaag ttcgcagacg cgggtgtcgc gctcgatagc gacgctgcga
    11101 cgcgcttcac gtcggtcgct gcgcacgtca tcgcggtcga gctcgacggc aacgcgccga
    11161 aggcgaagtt cgacctcggc ctgggcgaaa ccaccgcgca catcgccccg gacatcgact
    11221 ccgtcgatcc gcgctcggag gacttcctcc tcgcaggcgc aacggtcaac gcgacgtacc
    11281 aagctaacgc gctgacgctc gacaacatct cgctcggcag caagacctcg cagctctcga
    11341 tcggcggtca agtcgcgcag acgatcgatc tcaacgcgac cgcaggccgc aagctcgccg
    11401 ccgtcatcac gtcgaacccc gccacgggcg aagaaacgct cgaagtcacc ccgcgcctcg
    11461 acctccagat gacgaccgac cacgcggtgc tcgccgacga agcgccgcgc tacgacgtga
    11521 cgcgcatcct cctcgacggc tcgctgcgcg gcatggccga ctcgagccag ctcgaggtcg
    11581 tgtcaggctc gttctcgatg acgaccaacc cgtcgcagta cggcttctcg gcgacggccg
    11641 gccagtgcgt cacgtcgacg tcggagtacg acccggtgac ctacgaggac tacgagctct
    11701 actccgtggg cgcctgcctc tagctcgcgt cgtcgtttcc tgattcgtct atgatcgagg
    11761 ctcgtgcgct acgcgagcct cgttctcgtt tgtggctgcg gacgcctctc gttcgagccg
    11821 ctcgccgacg cgcccgaacg cgccgccgac gcgcccgccg atgcgccaac accaccctgc
    11881 ctcgtcgagg atttctcgac ggacaccggc atgtggcagc cggtgctcgg ctcgtacagc
    11941 ctgcaaccag gtgccggccc cgatggctca cccgcctacg tcggcgcgat ccaacccgaa
    12001 aatctcgcga ccaacgccgc gctccgcggc attcgcacgg cgcgcgtccg cctcgacttc
    12061 cgcatcgaca ccgcgaacgg cgacttcaat gtcttctggt tcaagccctt cacgtcgtac
    12121 gcgaccgcga ccggctacga agccctcctc gttcccgcca acagcgacac gctgaatgac
    12181 gccctcggcg tctggacgaa cggtagcgtg cagacattcg cgcaacgccc tggaacgatc
    12241 gtcatgtcga cgtggcatca catcgacgcc gagcttcgtg ccgacggctc gatgcagctc
    12301 gacctcgacg gcgcaccatg gctgtcatcg ccgcctgacg ccaccgtctc gccgcccttc
    12361 gacgtaatcc ttcgactgtt ctatcaaggc gcgatcgaca acatctccgt cgactgcaca
    12421 cgctaggtta tgctcgtcga cggtgc
//
LOCUS       DATLBZ010000314        15360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1102666, whole genome shotgun sequence.
ACCESSION   DATLBZ010000314 DATLBZ010000000
VERSION     DATLBZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15360
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102666"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VMZ53_16225"
     CDS             <1..223
                     /locus_tag="VMZ53_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HUS30055.1"
                     /translation="GGVIVSYYEWLQNKRAEQWEHADVLGRLERRMKQTYANVRQYAT
                     AQKTDWRTASYAIGLERLQHCYKERGIFP"
     gene            391..2298
                     /locus_tag="VMZ53_16230"
     CDS             391..2298
                     /locus_tag="VMZ53_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HUS30056.1"
                     /translation="MSTSLCHRKPSGSYQFLLDTILEEHATHPGDFVSSDASCVRPHI
                     TASLVEGPGVLSGAAPHGLATADFNRDGIRDLIATNLYDNTVSVMRGLGAGQFAPRTT
                     YGVPFPKHLEVADLDGDGLTDVVVSGPGNAVYTLRGNGDGTLQAARELFIGGSIESMT
                     LADIDRNGRPDLIVTNQSNMNNVMVALGNGDGTFGAPRVYAVGQTPVKVIAADVDGDR
                     KLDIITANYSDDTFSVLMNKGNGVLAPAITSWAGPTPSSIAAGDLDLDGKLDLVIANR
                     SGTVARFSGNGNGTFGFQSFTWVGPSTGAVAIADISGDGAPDVIVTDDFSHNVALLHG
                     YGTGQLDAPILLHLPSELREFTIADFDGDAKPDIAVAVFAGNYVALLRSGLRAYPLSI
                     TLAGAGTGRILGGPRLGQLCPGTCGGTFREGDVVTLSPFADAGSQFVGWSGACSGTGV
                     CRVTMNAAKSVTATFEPAVTFALTTHIVRVNDGWGNIYLGPAYGATSDGSTLFTAGTV
                     LTLAPLVSPGSTFLGWSGACSGTGTCTVTMDAAKDVSAIFDGAPQINFMVTFGIEGGG
                     SIFGGPMTGTLCPGACSKTYPLSSAATFVAMPDAGFVFSHWLGLCAGQGATCSVIVGQ
                     NTSTTAVFVAQ"
     gene            2327..3340
                     /locus_tag="VMZ53_16235"
     CDS             2327..3340
                     /locus_tag="VMZ53_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS30057.1"
                     /translation="MALFTLARWLGPWADSTKAPTVETRDEVVAGIKTRFYGRGRKRV
                     VIAPGLHYAAADDSRMDRFCRIVAASGSEVIAPFIPDYLKLTPGNQAKRDFTAVFDEI
                     AKTGDKPIVFSISFGSLLAFALAAERGEQIERLIIFGGYSDFHAVMRFCLTGEVASGR
                     KATRDPLNQPVVLMNLLHILEHDRSQTPALVEGWMTYVRRVWGRAHFKVDRAFVPVAE
                     EIAPSVPESVRELYRVGIGVVPGAVEIAMPALEKFDATALDPMPYLACVKNRVDLVHG
                     MDDDVIPFEQSHELAARLVNADVRVHITGLYGHTGTQRLPIGAMAKELVTMVRVLRVL
                     SAS"
     gene            3392..6088
                     /locus_tag="VMZ53_16240"
     CDS             3392..6088
                     /locus_tag="VMZ53_16240"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30058.1"
                     /translation="MAGDNESKTAIALVDPLVGTIFDKRFRIEERIAAGGFGAIYRAT
                     HVKSGHPIALKVLLPLLAQDLGIVARFRREGDTLTALRNPHTITAYELGQAGDRTLFI
                     VMELLSGQSLFERYEQNGPFEWRRMARIARQVCESLDEAHARGIVHRDLKPTNIHLEN
                     VDGDPDFVKVLDFGIAKILGGSDFDASDITNAGTMIGTLDYMSPEQMVGGQVTGQTDI
                     YTLGIVMYEMIAGVRPFPEAVSAASALGAMLKTTPQPLYLRAPVPEQISKIVMRCLER
                     EMQRRYRSVADLVKDLDEVIGEGGSANVVLDATKTFKPDDEATAFTPPPEKLLRELRT
                     PSAARMPEWEDEDEQATTLGGDRHADRAPSRAETNVLDGVDPHGPTVAARVPRNLDMP
                     GAKTPTVPRNLDGPGAKTPTTRRDVDTGGAKTVVRREADVAAAKSAPARADSTDLGAK
                     TPTARRDVDDVGAKTPTARRDSNELGAKTPTARRDPDAPAGRNRLDSDELDLRGNPDS
                     DRDDVAPRGRLESDGLDVRAARGRLESDGLDVRTPPFQRVDALEVETAPRPRSDLDDD
                     DDDGADIAGARLSRTSESRSQRDGVLSGAIDSDAQRGSRGTSATRPPQTPDAAAQRGS
                     RGSSATRPPQTPDPDAPRSKSATRPPSAFDADAPRPKSGTRPPPTPDPDAAGPRGKSA
                     SAPPPIAPTPPVRPLQARNYPTPVPQVVVAPFSSTPRAKTAPPTGMPTPPPGSLPPGV
                     PMPPMRQTPFPGAPPMGSPTNAPPNAHVPTPPGMAPYGAPNPNPFAPNAMPGSNPAGN
                     PFAVPGSNPSANPFAVPGSNPAANPFAVPGSNPAANPFSPPAPQSNPFLPSLPPAAHP
                     VMPRPQYDMGHMAAREAMIRRVVWIIALVVAAAIGFIIAMRF"
     gene            complement(6114..7565)
                     /locus_tag="VMZ53_16245"
     CDS             complement(6114..7565)
                     /locus_tag="VMZ53_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUS30059.1"
                     /translation="MRLALPCLLLLAACGTDVREEPDPPAPKVTWHQDVAPIVAKHCM
                     GCHQDGGIGPFDLTTYESASENAGNMLDKIEKGVMPPFDAREEADCTPRYSWKDDPRL
                     SDTDIATIQQWIADGTPEGTVAPLPPLPETSLPGITKTLKPIVPFTAAGNRDQFICYV
                     LDPNVPNGAWLTGLQIRPGNDLVVHHVVLTEITPSAETDALVAQRGIGVPWDCDQTGT
                     PGAFVVNIWTPGNEPMQTSNDLAVPLVANSKLVMNIHYHPAGGIHQPDATEVDLRTST
                     AWPKKMYFVTAFGNEFQAPNLLADPDDRLTTTPEFRIPANKALHTEHMRTTIPSLGNL
                     TDVRLYSANPHMHMIGTHISAHIERPAPRLGQPQNECLANGNWNFDWQRTYIYDAPLD
                     QLPSVAEGDVIDLKCNWNNTMDNPFVQRALVDAGLGAPIDINLGEGSLDEMCLEIFGI
                     AIDAPAQPLARPTQLAPSVEQLPLELLDAMRVD"
     gene            7693..8181
                     /locus_tag="VMZ53_16250"
     CDS             7693..8181
                     /locus_tag="VMZ53_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30060.1"
                     /translation="MSRVLVCVAVMAAACSGGSKEVKRTGNGGGNGNVGQGSGTGSAT
                     KPAGTGSGAVAVVPDVGCLVTGCSYHAGTGTYLTCLAGGAGTCFHFGGPCAPADQCMF
                     DNADKTYKQCSQIVEGACAQWGAACSPAKACMYSPTDGLHHTCDDVAGGKCRKYGALC
                     AP"
     gene            complement(8183..9184)
                     /locus_tag="VMZ53_16255"
     CDS             complement(8183..9184)
                     /locus_tag="VMZ53_16255"
                     /inference="COORDINATES: protein motif:HMM:NF039709.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HUS30061.1"
                     /translation="MRSLVVAVAVLAIAGCSKGSDHGANVEPPDLQMVSTGNEPRQVL
                     RYRIPKGTSQGIEVTVDMNVSAGDMGGPLPTITMSMLVAVEDVQAGTGAMKLHTTIVD
                     ATARDRDDAKISATALAGPLEKMKGLALAATLSPNGRTSKSEVEGMKQLPDEMAQQLG
                     ALTSTLERVMMPLPNEPVGVGAVWRSSRDITQNGMKMTTVNTFTLVGFDGAKVTYTID
                     TDVHGADQTVKLGEDSVQVKDITGTGGGNGAFSLTSLEMTSVLSAEFRSQMAVPGEAV
                     PTPMKMISQLKVAPQNSPTPTPPPTPAPAAGSAGSAGSAGSAGSAGSAGSAGSGSGS"
     gene            9312..9536
                     /locus_tag="VMZ53_16260"
     CDS             9312..9536
                     /locus_tag="VMZ53_16260"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HUS30062.1"
                     /translation="MIVCLCHPTSERDVDSIIDEGARTVEDIGRRCGAGTGCGACVGE
                     LHERLAAKGAGGGSCPRDREQNDLVPLRAR"
     gene            9618..10094
                     /gene="bfr"
                     /locus_tag="VMZ53_16265"
     CDS             9618..10094
                     /gene="bfr"
                     /locus_tag="VMZ53_16265"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HUS30063.1"
                     /translation="MKSTEEILTLLNQLLTNELTAINQYFIHAKMCENWGYERLAHKV
                     REESIDEMRHADQVISRILFLEGIPNLQALHKLRVGETVKEQLESDLQLEYNAIGFLN
                     KAVEAARAAHDTATEDLMRRILVSEEEHTDWIETQLELIRQVGEQNYLSQQIKKTS"
     gene            complement(10180..10566)
                     /locus_tag="VMZ53_16270"
     CDS             complement(10180..10566)
                     /locus_tag="VMZ53_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30064.1"
                     /translation="MLLFRSLALGLLGACFFMLATRPTTIVVRQPPPIEVPNLSGIRG
                     VPLPAVSEVAPTVIDVAPNVNASMLTTLIMMRDGEHVIAVNDARVANDLDAGRMLGAL
                     WLHPQRYIDVQLGGPDGERRVLVLAH"
     gene            complement(10742..12550)
                     /locus_tag="VMZ53_16275"
     CDS             complement(10742..12550)
                     /locus_tag="VMZ53_16275"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30065.1"
                     /translation="MQNSPARAGIDVGSVIDGTYTIEALIGRGGMGAVFLASHTRLPG
                     KKVAIKVLHAEMSGDEVLARFKREAQIASMLSHPNIVHIEDYKITADGMPYLVLEYLQ
                     GESLAQRLKRGPLAPEQAFSIIRQVGSALAAAHQKGIVHRDLKPQNIFLVPSEIDGRA
                     FEVAKVLDFGISKMRDSQTVKTQDSALLGTPQYMAPEQATGQHANVDERTDVFALGAI
                     VYEMLAGQPAFSGASIPEVVFKVVYEQPTQLTSQVPTLSAQIATAVHRAMSKPAGERF
                     STVSDFVEALTGHPLPAPRSGNSLPPPDVGVATSSRTPDTGKEAFANTMGSGDHGAAM
                     ANTIDSGNRPGATAAQAAAGTTATPPIAGAMVTPSPVHGNAPTMSSQSGQPLGLAPTM
                     SPPRRPRAAMFIAGIAAVAAAAAIAFFALKSKDPEKPVPVEKAAQVTPHDEAIAPVKE
                     PPREDVVAKADVVDAGVQPEASAVDAKGETPKIAKSAKIAKTASATTTATTKTDDEGE
                     GDESAKQKLNDADAALKASQWARAEQLANAVVNSEDAFPKQKARASMIHGMAQCLGRN
                     NEEGALTDLRRIKGAPALRVKLLKTCQKAGFMLAEH"
     gene            12578..13372
                     /locus_tag="VMZ53_16280"
     CDS             12578..13372
                     /locus_tag="VMZ53_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30066.1"
                     /translation="MRAGPMGGGLQLWCVAACLLLSSSALAEPKPKAVDIKPFRDKLI
                     VLQDTDGGTYVVLPGDAPRIWYGVAKKPLYEQIGIGRSADHEKWSVSVFAPRVPKWQP
                     GSIDFKGAGEYARYCGGDAKIPLTELSSDKAKTILDKSSFMSTAFLRRPHLLARDDSG
                     VYYYVDAIRDEYGGNGFRVLVGKKGALKQKPLTDIASDSAGDVFATKTGELRFVKTVA
                     DGKTTTYWVKGGKKTELTTLDIEQNERMIYRDLGVYSFTGSICENM"
     gene            13357..14172
                     /locus_tag="VMZ53_16285"
     CDS             13357..14172
                     /locus_tag="VMZ53_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30067.1"
                     /translation="MRKHVALLVALGSSIASAGPSKTDDKAPAAATTIDVAPVAEKLA
                     AYKDEFGNIYVMPALAAFDDTNELQKWFFYGDGKTMYQQRVSRFTTGKGGYDVSLVAP
                     RAKEVPGGWLVNNSDGLSLTCRAARVSGGRRDLTALSADETKTLLAKAKFVPPMLLRQ
                     PHFLGRDDDASYYYIDRLASEAGGKGFRVYVGQTGAMKQLALTNMATDSMGEIYATKS
                     GKLKITTGKDAHAYWIKGSKKIELTVVPPADNRYLVYRELGIYGSLGTVCDDQ"
     gene            14185..14946
                     /locus_tag="VMZ53_16290"
     CDS             14185..14946
                     /locus_tag="VMZ53_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS30068.1"
                     /translation="MKLSGSVALVTGGASGLGAATVRHLVTAGAKVVIVDREEARGSA
                     LASELGQTFAKADVTDAAQVEAAIATASGLGPLRVVVSCAGVGWAARTLDKTGKPHDL
                     ELFKTVVGVNLIGTFNVLRLGASAISKTDPLEHGERGVIVNTASVAAFDGQIGQIAYA
                     ASKGGVAAMTLPAARDLAPAGIRVITIAPGIFDTPMLGALPEEKRAALASDVVFPKRL
                     GDPSEYGALVRAIVENGYLNGETIRLDGSLRMPPK"
     gene            14993..>15360
                     /locus_tag="VMZ53_16295"
     CDS             14993..>15360
                     /locus_tag="VMZ53_16295"
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HUS30069.1"
                     /translation="MRRLAMVPLVLFVVACSSKKPDAPAGGSAAPVSAARPDAAPAVT
                     AARDARASTPDAPGRPSDDAVAGFGVGDDGRGLYYDRPLAPADLDGRSLRDLTLMRNV
                     VYARAGHPFRKAWLREYFTA"
BASE COUNT         2543 a         5071 c         5140 g         2606 t
ORIGIN      
        1 gggcggcgtg atcgtcagct actacgagtg gctgcagaac aagcgcgccg agcagtggga
       61 gcacgccgac gtcctcggcc gcctcgagcg ccgcatgaag cagacgtacg cgaacgttcg
      121 ccaatacgcg accgcgcaga agacggactg gcgcacggcg agctacgcga tcggtctcga
      181 gcgcctgcag cactgctaca aagagcgcgg catcttcccg tagccgtgag cttcgcgtgt
      241 tgctgcgtga agcacgcggt aactcgcacc tctcgctgca tcgcagtgcg tagtgtgtcg
      301 ctcatgaagc gatacacagc atgcgtatgg gcggcgttgg caatgtccgg cgccgcatgc
      361 accgacgaac tggccgacac gtcgcagtcg gtgtcgacca gtctttgcca ccgtaagcca
      421 tcggggagct accagttctt gctggacacg atcctcgagg agcacgcgac ccatccaggc
      481 gacttcgtgt cgtcggatgc gagctgtgtg cgcccgcaca tcacggcctc acttgtcgaa
      541 ggcccgggcg ttctcagcgg agcggcgccc catggtctcg cgacggcgga cttcaatcgc
      601 gatggcatcc gcgatttgat cgcgaccaac ctctacgaca atacggtgtc cgtgatgcgc
      661 ggcctcggcg ctgggcagtt cgctccgcgc acgacgtacg gcgtcccgtt tccgaagcat
      721 ctggaagttg cagacctcga cggcgacgga ttgaccgatg tcgttgtcag cggtccggga
      781 aatgccgtct acacgttacg tggcaacggc gatgggacgc tgcaggcggc gcgcgagctc
      841 ttcattggtg gctcgatcga atcgatgacg ctcgctgaca tcgatcgcaa tgggcgtcct
      901 gatctcatcg tcacgaatca gtcgaacatg aacaacgtga tggtcgcgct cggcaatggc
      961 gacggcacat tcggcgcgcc gcgcgtgtat gcggttggcc aaacacccgt gaaggtgatc
     1021 gccgcggacg tcgatggtga tcgcaagctc gacatcatca cggcgaacta ttcggacgac
     1081 acgttctcgg tgctgatgaa caaaggtaac ggtgtgctcg caccggcgat cacgtcatgg
     1141 gccggtccga cgccgagctc catcgctgca ggcgacctgg acctggacgg caagcttgat
     1201 ctcgtgatcg cgaatcggag tggcaccgtc gcgcgcttta gcggtaacgg gaacgggacg
     1261 ttcgggtttc agagcttcac gtgggtcggc ccgtcgacag gtgctgtcgc catcgcggac
     1321 atcagtggtg acggcgcgcc cgacgtgatc gtcacggacg acttcagcca caacgtggcg
     1381 ttgcttcacg gctacggcac gggccagctc gacgcaccga tcctgctgca tctgccatcg
     1441 gaattgcgcg aattcacaat cgcggacttc gacggcgacg cgaagcccga cattgccgtg
     1501 gcggtgtttg cgggcaacta cgtcgcgctt ctgcgctcgg gactacgcgc gtatccgctc
     1561 tcgattacgc tcgcaggcgc tggcactgga cggattttgg gtggtccgcg tctcggacag
     1621 ctttgcccgg ggacgtgcgg cggaacgttc cgcgaaggcg atgtcgttac gctgtcgcca
     1681 ttcgccgacg ctgggtcgca gtttgtcggc tggagtggcg cgtgctcggg cactggcgtg
     1741 tgccgggtga cgatgaatgc agcgaaatcg gtgaccgcga cgttcgagcc cgccgtcacg
     1801 ttcgcgctga cgacgcacat cgttcgcgtc aacgatggct ggggcaacat ctacctgggg
     1861 ccggcgtatg gcgcgacatc ggacggttcg acgctcttca cggcgggtac agtccttacg
     1921 ctcgcgccgt tggtctcccc gggttcgaca ttccttggct ggagcggcgc gtgcagtggc
     1981 acgggaacct gcaccgtcac gatggatgcc gcgaaggacg tgagcgcgat cttcgacggc
     2041 gcgccccaga tcaacttcat ggtcacgttt ggcatcgaag gcggcggatc gatcttcggc
     2101 ggtcccatga caggcacact gtgccctggc gcgtgcagca agacgtatcc gctgagctca
     2161 gcggcgacgt ttgttgcaat gcccgatgca ggcttcgtgt tctcgcactg gctcggtctt
     2221 tgtgccggcc aaggcgcgac ctgctcggtc atcgtcggac aaaatacgtc gacaacggct
     2281 gtgttcgtcg cgcagtagca cggcgagacg tcggtatagt gcaggcgtgg cgctcttcac
     2341 gctcgctcgt tggctcgggc catgggccga ctcgacgaag gcgccgaccg tcgagactcg
     2401 cgacgaggtc gttgccggaa tcaagacgcg cttctacggc cgcggtcgca agcgcgtcgt
     2461 gatcgcgccg gggttgcact acgcagccgc cgacgattcg cggatggatc ggttctgccg
     2521 gatcgtcgcg gcgagcggct cggaagtgat cgcgccgttt atcccggatt acctgaagct
     2581 cacgccgggc aatcaggcga agcgcgactt caccgcggtg ttcgacgaga ttgcgaagac
     2641 gggcgacaag ccgatcgtgt tttcgatctc gtttggctcg ttgctcgcgt ttgcgctggc
     2701 ggcggagcgc ggcgagcaga tcgagcggct catcatcttc ggcgggtatt cggactttca
     2761 tgcggtgatg cggttctgtt tgaccggtga ggtcgcgagc gggcgcaagg cgacgcgcga
     2821 tccgctgaat cagccggtcg tgctgatgaa cctgttgcac atcctcgagc acgatcgatc
     2881 gcagacgccg gcgctcgtcg agggctggat gacgtacgtg cggcgggtgt ggggacgcgc
     2941 gcatttcaaa gtcgatcgcg cgttcgtgcc ggtggcggag gagatcgcgc cgagcgttcc
     3001 ggagagtgtt cgcgagctgt atcgcgttgg catcggcgtc gtgccgggcg cggtcgagat
     3061 cgcgatgcct gcgctcgaga agttcgacgc gaccgcgctc gatcccatgc cgtatctggc
     3121 gtgcgtgaag aaccgcgtcg acctcgtgca tggcatggac gacgacgtga tcccgttcga
     3181 gcaatcgcac gagctcgcag cgcgtctcgt gaatgcggat gttcgtgtcc acatcaccgg
     3241 cttgtatggc cacaccggca cgcagcgcct gccgatcggc gcgatggcga aggagctggt
     3301 caccatggtc cgcgtgctcc gagtgctttc agctagttag aatcgggcca ctaaatcggc
     3361 gcggcgctcg gggcgaagta cagttgacgt cgtggctggc gacaacgagt cgaagaccgc
     3421 gatcgcgctg gtcgacccgc tggtcggcac gatcttcgac aagcgcttcc gcatcgaaga
     3481 gcggatcgct gcgggcggct ttggtgcgat ctatcgcgcg acgcacgtca agagcggcca
     3541 cccgatcgcg ctgaaggtcc tgctgccact cctcgcgcaa gacttgggca tcgtcgcgcg
     3601 cttccgccgc gaaggcgaca cgctcaccgc gctacgcaat ccgcacacga tcacggcata
     3661 cgagctcggg caggctggcg accgcacgct cttcatcgtc atggagctgc tcagcggcca
     3721 gagcctgttc gagcggtacg agcagaacgg tccgttcgag tggcgacgca tggcgcgcat
     3781 cgctcgccaa gtctgcgagt cgctggatga agcgcacgcg cgaggcatcg tgcatcgcga
     3841 tctgaagccg acgaacatcc acctcgagaa cgtcgacggc gatccggact tcgtcaaagt
     3901 actcgacttc gggatcgcga agatcctcgg cggctcggac ttcgacgcgt ccgacatcac
     3961 gaacgccggc acgatgatcg gcacgctcga ttacatgtcg cccgagcaga tggtcggcgg
     4021 ccaagtgacc ggccaaaccg acatctacac gctcggcatc gtcatgtacg agatgatcgc
     4081 gggcgtgcgt ccgttccccg aggcggtctc ggctgcctcc gcgctcggcg cgatgctcaa
     4141 gacgacgccg cagccgctct acctgcgcgc gcccgtgccc gagcaaatca gcaagatcgt
     4201 gatgcgctgc ctcgagcgcg agatgcagcg ccgctatcgc tccgtcgccg acctcgtgaa
     4261 ggatctcgac gaggtaatcg gcgaaggcgg cagcgcgaat gtcgtcctcg acgcgacgaa
     4321 gacgttcaag cccgacgacg aagccaccgc gttcacgccg cctcccgaaa agctgctccg
     4381 cgagctgcgc acgccgagtg cagcgcgcat gcccgaatgg gaggacgaag acgagcaggc
     4441 gacgaccctc ggcggtgacc gccacgccga ccgcgcaccg tcgcgcgcgg agacaaacgt
     4501 cctcgacggc gttgatccgc acggcccgac ggtcgccgca cgcgttccgc gcaatctcga
     4561 catgcccggc gcaaagacgc caaccgttcc gcgcaacctc gacgggccgg gcgcaaagac
     4621 gccgacgacg cgccgcgatg tcgacacggg cggcgccaag acggtcgtgc ggcgtgaagc
     4681 cgacgtggct gccgccaaga gcgcaccagc gcgcgccgat tccacggacc tcggagcaaa
     4741 gacgccgaca gcacgccgcg atgtcgacga tgtgggcgcg aagacgccga cggcgcgccg
     4801 cgattccaac gagctcggcg cgaaaacgcc gacagcgcgc cgcgatcccg acgcgccagc
     4861 tggccgcaac cggctcgaca gcgatgagct cgacctgcgc ggcaacccgg atagcgatcg
     4921 cgatgacgtc gcgcctcgcg gtcggctcga gagcgatggc ctcgatgtcc gcgcggctcg
     4981 cggtcggctc gagagcgatg gcctcgatgt ccgcacgccg ccgtttcaac gcgtcgacgc
     5041 tctcgaggtc gagacggcgc cgcgtccgcg ctcggacctc gacgacgatg atgatgacgg
     5101 cgcggacatc gcgggcgcgc gcctcagccg cacgtcggag tcgcggtcgc agcgcgacgg
     5161 cgtgctgagc ggcgcgatcg attccgacgc gcagcgagga tctcgcggca cctccgcgac
     5221 gcgtccaccg cagacgcccg acgccgccgc gcagcgagga tctcgcggca gctccgcgac
     5281 gcgtccaccg cagacgccgg accccgacgc accgcgatcg aagtccgcga cgcgacctcc
     5341 gtccgcgttc gatgcggacg caccacggcc gaagagcggc acgcgcccac cgccgacgcc
     5401 ggatcccgac gccgctgggc cgcgcggcaa atccgcgagc gcaccgccgc cgatcgcgcc
     5461 gacgcctccc gtgcggccgc tgcaggcgcg caattatccg acgccggtgc cgcaggtcgt
     5521 cgtcgcgccg ttctcgtcga cgccgcgcgc gaagacagcg ccgccgacgg gcatgccgac
     5581 gccaccgccc ggcagcttgc cgcccggcgt gccgatgccg ccgatgcgtc agacgccgtt
     5641 tcccggcgcg ccgccgatgg gctcgccgac gaatgcgccg ccgaacgcgc acgtgccgac
     5701 accgccggga atggcgccgt atggtgcacc gaatccaaat ccattcgcgc cgaatgcaat
     5761 gccgggcagc aacccggcgg gaaatccgtt cgcggtcccc ggcagtaatc cgagcgcgaa
     5821 tccattcgcg gtgcccggca gcaatcccgc cgcgaatcca ttcgcggtgc ccggcagcaa
     5881 tcccgccgcg aatccgttct cgccgccggc gccgcagagc aatccgttcc tgccgtcatt
     5941 gccgccggcc gcgcatccgg tgatgccgcg acctcagtac gacatgggcc acatggccgc
     6001 acgcgaagcg atgatccgcc gcgtcgtttg gattatcgcg ctcgtcgtcg ccgccgcgat
     6061 tggcttcatc atcgcgatgc gcttctagcg agcgcgcgcg gtgacggttg cgatcagtcg
     6121 acgcgcatcg cgtcgaggag ctcgagcggc agctgctcga cgctcggcgc gagctgcgtc
     6181 gggcgggcga gaggctgcgc aggcgcgtcg atcgcgatgc cgaagatctc gaggcacatc
     6241 tcgtcgagcg agccttcgcc gaggttgatg tcgatcggcg cgccgaggcc cgcgtcgacg
     6301 agcgcacgct gcacgaacgg gttgtccatc gtgttgttcc agttgcactt caggtcgatg
     6361 acgtcacctt cggcgaccga cggcagctgg tcgagcggcg cgtcgtagat gtacgtgcgc
     6421 tgccagtcga agttccagtt gccgtttgcg aggcactcgt tctgcggctg accgaggcgc
     6481 ggcgcgggac gctcgatgtg agccgagata tgcgtaccga tcatgtgcat gtgcgggttc
     6541 gcggagtaga ggcgcacgtc cgtgaggttg ccgagcgacg gaatcgtcgt ccgcatgtgc
     6601 tcggtgtgca gcgccttgtt tgccgggatg cggaactcgg gcgtcgttgt gaggcgatcg
     6661 tccggatcgg cgaggaggtt cggcgcttgg aactcgttgc cgaacgcggt cacgaagtac
     6721 atcttcttcg gccacgccgt ggacgtgcgc agatcgactt cggtcgcgtc cggctgatgg
     6781 atgccaccgg cggggtggta gtggatattc atcacgagct tcgagttcgc gacgagcggc
     6841 acggcgagat cgttcgacgt ctgcatcggc tcgttgccgg gcgtccagat gttgacgacg
     6901 aacgcgccgg gcgtacccgt ttgatcgcag tcccacggca cgccgatgcc acgttgcgcg
     6961 acgagcgcat cggtttcggc gctcggggtg atctcggtga gcaccacgtg atgcacgacg
     7021 aggtcattgc ccgggcgaat ctgcaggccc gtgagccatg cgccattcgg cacgttcgga
     7081 tcgagcacgt agcagatgaa ttgatcgcgg ttgcccgccg ccgtgaacgg cacgatcggc
     7141 ttcagcgtct tcgtgatgcc gggcagcgac gtctcgggca gcggcggtag cggcgcgacc
     7201 gtgccttccg gtgtgccgtc tgcgatccat tgctggatcg tcgcgatgtc ggtgtcggac
     7261 aagcgcggat cgtctttcca cgagtagcgc ggcgtgcagt cggcttcttc gcgcgcgtcg
     7321 aacggcggca tcacgccctt ctcgatcttg tcgagcatgt tgccggcgtt ctcggatgcg
     7381 gactcgtagg tcgtcagatc gaacggtccg atgccgccat cctgatgaca gcccatgcag
     7441 tgcttcgcga cgatcggcgc gacatcctga tgccaggtca ccttcggcgc cggcgggtca
     7501 ggctcctcgc gcacgtcggt accgcaggca gcgagcaaca gcaggcaggg gagggcaagg
     7561 cgcatgggtt gggcttgcag caaagcgtat acccgccgat cgcgtggggg aatccgacgc
     7621 ctgtgtgggg tatccccacg ctgtgaacgt ccgtggcggg cattcgcgct cccgcgacgc
     7681 tatagtcgtg cggtgtcacg tgttctggtc tgcgttgcgg tgatggcggc tgcctgcagc
     7741 ggtggctcca aagaggtcaa acggaccggc aacggcggcg ggaacggcaa tgtgggacaa
     7801 ggctctggca cgggcagcgc gacgaagcca gcgggcaccg gctcgggcgc ggtcgccgtc
     7861 gtgcctgacg tcggctgcct cgtgacgggc tgcagctatc acgcgggcac cggcacgtac
     7921 ctcacgtgtc tcgccggtgg cgcgggcacc tgctttcact tcggtggtcc gtgcgcgccc
     7981 gccgatcaat gcatgttcga caacgccgac aagacgtaca agcagtgctc gcagatcgtc
     8041 gaaggcgcct gcgcgcagtg gggcgctgcg tgctccccgg cgaaagcatg catgtactcg
     8101 ccgaccgatg gtctgcacca cacgtgcgac gacgtcgcgg gcggcaagtg tcgcaagtac
     8161 ggcgcgctct gcgctccgtg agctagctgc ccgagccgct gccggcggaa ccggcggaac
     8221 cggcggaacc ggcggaaccg gcggaaccgg cggaaccggc ggaaccggcg gcaggcgcgg
     8281 gcgtcggtgg cggggtcggc gtcggtgaat tctgcggcgc gaccttcagc tgcgagatca
     8341 tcttcatcgg cgtcgggacc gcttcgcccg gcacggccat ctggctgcga aactctgccg
     8401 acagcacgct cgtcatctcg agcgacgtca gcgagaacgc gccgttgcca ccgccggtgc
     8461 cggtgatgtc tttgacctgc acgctgtctt cgccgagctt caccgtttga tcggcgccgt
     8521 gcacgtcggt atcgatcgtg tacgtgacct tcgcgccgtc gaagccgacg agcgtgaacg
     8581 tgttcaccgt cgtcatcttc atgccgttct gcgtgatgtc gcgcgagctg cgccacaccg
     8641 cgccgacgcc gacgggctcg ttcggcagcg gcatcatcac gcgctcgagc gtcgacgtca
     8701 gcgcgccgag ttgctgcgcc atctcgtccg gcagctgctt catgccttcg acttcgctct
     8761 tcgatgtgcg tccattcggc gacaacgtcg ccgcgagggc gaggcccttc attttctcga
     8821 gcgggccggc gagcgcagtc gccgaaatct tcgcgtcgtc gcgatcgcgc gcggtcgcat
     8881 cgacgatcgt cgtgtgcagc ttcatcgcgc cggtgcctgc ttgcacgtct tcgaccgcga
     8941 ccagcatcga catcgtgatc gtcggcagcg gcccgcccat gtcgccggcg ctgacgttca
     9001 tgtcgacggt gacctcgatg ccctgcgagg tgccctttgg aatgcgatag cgcagcacct
     9061 gccgcggctc gttgccggtg ctcaccatct gcagatccgg cggctcgacg ttcgcgccat
     9121 gatcgctgcc cttactgcac ccggcgatcg ccaggaccgc aactgcaacg accagactcc
     9181 gcatacttac aaattggggt cgcgcagagg gcccgaagcg catgccgcaa gtttagtaaa
     9241 tccaggcgct tgcgcgccac atttcgacac cttgacggtt ctggttttga aatgcattat
     9301 caaattcaga catgatcgtt tgcctgtgtc accccacgtc ggagcgcgac gtagactcga
     9361 tcatcgacga gggtgcgcgc acggtcgaag acatcggtcg tcgctgcggc gctggcaccg
     9421 gctgcggcgc atgcgtcggc gagctgcacg agcgcctggc ggccaagggt gccggcggcg
     9481 gctcttgtcc gcgcgaccgc gaacagaacg atctcgtccc gctgcgcgcc cgctgaattt
     9541 cagcgtcccg atcccgagcg ccgattccgc gcgcatcgca tgtcgatcca cgcacggcga
     9601 gcgtagtctc ggtcgacatg aagagcacgg aagagatcct gacgctcttg aatcagctcc
     9661 tcacgaacga gctgaccgcg atcaaccagt acttcatcca cgccaagatg tgtgagaact
     9721 ggggttacga gcggctcgcg cacaaggtcc gcgaagagtc gatcgacgag atgcgccacg
     9781 ccgatcaagt gatcagccgc atcctgtttc tcgagggcat cccgaatctg caggcgctcc
     9841 acaagttgcg cgttggcgag accgtcaaag agcagctcga gagcgatctg cagctcgagt
     9901 acaacgcgat cggctttctc aacaaagcgg tcgaagccgc gcgcgccgcg cacgacaccg
     9961 cgaccgaaga tctgatgcgc cgcatcctcg tctccgagga agagcacacg gactggatcg
    10021 agacgcagct cgagctcatc cgccaagtcg gcgagcagaa ctacctctcg cagcagatca
    10081 aaaagacctc gtagtccatc gcgatcgcgg gcatcgcgag cccgatcgcc gcgcgacgag
    10141 cgttagtgcg cgagcacgcg acgacggcgt cgacgagctc tagtgcgcga gcacgagcac
    10201 gcgccgctcg ccgtcgggtc cacccagctg cacatcgatg tagcgctgcg ggtgcagcca
    10261 gagcgcgccg agcatgcggc ccgcatcgag gtcgttcgcg acgcgcgcat cgttgaccgc
    10321 gatgacatgc tcgccatcgc gcatcatgat cagcgtcgtc agcatgctcg cattgacgtt
    10381 cggcgcgacg tcgatgaccg tcggagcgac ctcgcttacg gccggcagcg gcacgcctcg
    10441 aatgcccgac agattcggca cctcgatcgg aggcggctgc cgcacgacga tggtcgtcgg
    10501 gcgcgtggcg agcatgaaga agcacgcacc gaggaggccg agggcaagcg accggaacag
    10561 cagcatgcca ggcagagttg caacgcgctg gccatcgcgc gccgtcggcg ctgcgcacgc
    10621 atctcgcgca gttaggcccc ggccgcgcgc gtggcgctgc ccgattgcca tcgcgtcgcg
    10681 ctgaccgctt gccgatctgg cagcgccgcc gtgcagagca gcagccgcac gctcgatgag
    10741 gtcagtgctc ggcgagcatg aagccggcct tctggcaggt cttcagcagc ttcacgcgca
    10801 gcgcgggcgc acccttgatg cgacgcagat ccgtcagcgc gccttcctcg ttgttgcgac
    10861 cgaggcactg cgccatgccg tgaatcatcg atgcgcgcgc cttctgcttc ggaaatgcgt
    10921 cctcgctgtt gacgaccgcg ttcgcaagct gctcggcgcg cgcccactgg ctcgccttga
    10981 gcgccgcatc ggcatcgttg agcttctgct tcgcggactc atcgccttcg ccttcgtcgt
    11041 ccgtctttgt cgtcgcggtt gtcgtcgcgc tcgcggtctt cgcgatcttc gcgctcttcg
    11101 caatcttcgg cgtttcaccc ttcgcatcca ctgcgctggc ctccggctgc acgcccgcgt
    11161 cgacgacgtc tgccttcgct acgacgtctt cgcgcggcgg ctctttgacg ggcgcgatcg
    11221 cttcgtcgtg cggagtcacc tgcgccgcct tctcgacggg caccggcttc tcgggatcct
    11281 tcgacttcag cgcgaagaac gcgatcgcgg cggctgcggc tacggcggca atgcccgcga
    11341 tgaacatcgc ggcgcgcgga cggcgaggcg gcgacatcgt cggcgcgaga ccgagcggct
    11401 gcccgctttg cgagctcatc gtcggcgcgt tgccgtgcac cggcgacggc gtgaccatgg
    11461 cccctgcgat cggcggcgtt gccgtcgtgc ccgcagcggc ctgcgcagcc gttgcaccgg
    11521 ggcggttgcc cgagtcgatg gtattggcca tcgccgcgcc gtggtcgccc gagcccatcg
    11581 tgttcgcgaa ggcttccttg cccgtgtccg gcgtgcgcga gctcgtcgcg accccgacgt
    11641 ccggcggcgg tagcgaattg cccgagcgcg gcgcgggcag cgggtggccc gtgagcgctt
    11701 cgacgaagtc actcaccgtc gagaatcgct cgcctgcggg cttcgacatc gcgcggtgca
    11761 ccgcggtcgc gatctgcgca gagagcgtcg gcacctgcga ggtcagctgt gtcggctgct
    11821 cgtagacaac cttgaacacg acctccggaa tcgacgcgcc gctgaacgcg ggttgaccgg
    11881 caagcatctc gtagacgatc gcgccgagcg cgaagacgtc cgtgcgctcg tcgacgttcg
    11941 cgtgctgacc ggtcgcttgc tccggcgcca tgtactgcgg cgtgccgagc agcgcgctgt
    12001 cctgcgtctt caccgtctgc gagtcgcgca tcttcgagat gccgaagtcg aggaccttgg
    12061 cgacctcgaa cgctcgacca tcgatctcgc tcggcacgag gaagatgttc tgcggcttga
    12121 ggtcgcgatg aacgatgccc ttttgatgcg cggcagcgag tgccgaaccg acttggcgaa
    12181 tgatcgagaa cgcctgctcg ggtgcaagcg ggccgcgctt gagacgctgc gcgagcgact
    12241 cgccctgcag gtactcgagc acgaggtacg gcatgccgtc cgccgtgatc ttgtagtcct
    12301 cgatgtgcac gatgttcgga tgcgagagca tcgaggcgat ctgcgcttcg cgcttgaagc
    12361 gcgcgaggac ttcgtcgccg gacatctcgg catgcaggac tttgatcgcg accttcttgc
    12421 cgggtaggcg ggtgtggctc gcgaggaaga ccgcgcccat cccaccccgg ccgatgaggg
    12481 cctcgatcgt gtaagtgccg tcgatgacgg aacccacgtc gatgccggcc cgggccggtg
    12541 aattctgcat gctcacggag cgtataatga gacaccgatg cgtgcaggtc ctatgggtgg
    12601 cggccttcaa ctttggtgcg tcgcggcgtg cctacttctg tcgagtagcg cgcttgccga
    12661 gccgaagccc aaggccgtcg atatcaagcc gttccgcgac aagctgatcg tcttgcaaga
    12721 taccgatggc ggcacctacg tcgtgctacc tggcgacgcg ccccgcattt ggtacggcgt
    12781 cgccaagaag ccgctctacg aacagatcgg aatcggccgg tccgccgatc acgagaagtg
    12841 gagcgtctcg gtgttcgcgc cgcgtgtccc gaagtggcag cccggcagca tcgacttcaa
    12901 gggcgccggc gagtacgcgc ggtattgcgg cggcgacgcg aagattccgc tcaccgagct
    12961 ctcgagcgac aaggcgaaga cgatcctcga caagagctcg ttcatgtcga ccgcgttctt
    13021 gcgccgcccg cacctgctcg cgcgcgatga ctccggcgtt tactactacg tcgatgcgat
    13081 ccgcgacgag tacggcggca acggctttcg cgtcctcgtc ggaaagaagg gcgcgctcaa
    13141 gcagaagccg ctgaccgaca tcgcatcgga ttctgcaggc gatgtgttcg caacgaagac
    13201 gggcgagctg cgcttcgtca agacggtcgc tgacgggaag accacgacgt actgggtgaa
    13261 gggcggcaag aagacggagc tcaccacgct cgatatcgaa cagaacgagc gaatgatcta
    13321 ccgcgacctc ggcgtctaca gcttcaccgg atcgatatgc gaaaacatgt agcgctgctg
    13381 gtcgcgctcg gctcgagcat cgcgagcgct ggaccgtcga agaccgacga caaagcgccc
    13441 gctgccgcga cgacgatcga tgtcgcgccg gtcgccgaga agctcgccgc gtacaaggac
    13501 gaattcggca acatctacgt gatgcccgcg ctcgccgcgt tcgatgacac gaacgagctg
    13561 cagaagtggt tcttctacgg tgacggcaag acgatgtacc agcagcgcgt ctcgcgcttc
    13621 acgacgggga agggcggcta cgacgtcagc ctcgtcgcgc cgcgcgcgaa ggaggtcccc
    13681 ggcggatggc tcgtgaacaa cagcgatggc ctgtcgctca cctgtcgagc ggcgcgcgtt
    13741 tccggtggcc ggcgtgacct caccgcgctc agcgccgacg agacgaagac gctgctcgcg
    13801 aaagcgaagt tcgttccacc gatgctgctg cgccagccgc acttcctcgg ccgcgacgat
    13861 gacgcgagct actactacat cgatcgcctc gcgtcggagg ccggcggcaa aggcttccgc
    13921 gtgtatgtcg ggcagaccgg cgcgatgaag cagctcgccc tgacgaacat ggcgaccgac
    13981 tcgatgggcg aaatctacgc gacgaagtcc ggcaagctga agatcacgac gggcaaggac
    14041 gcgcacgcgt actggatcaa gggcagcaag aagatcgagc tgaccgtcgt gccgcccgcc
    14101 gacaatcgct acctcgtgta tcgggagctc ggcatctacg gctcgctcgg caccgtctgc
    14161 gacgatcagt agtactgtcg ccgcatgaag ctctccggtt cggttgctct cgtcacgggt
    14221 ggtgcgtcgg gcctcggtgc tgcgacggtg cggcatctcg tcaccgccgg cgcgaaggtc
    14281 gtgatcgtcg atcgggaaga agcgcgcggc agcgcactcg caagcgagct cggccaaaca
    14341 ttcgcgaagg ccgacgtcac cgacgctgcg caagtcgaag cggcgatcgc aacggcgtcg
    14401 gggctcggcc cgctgcgcgt cgtcgtgtcg tgcgccggcg tgggctgggc tgctcggacg
    14461 ctcgacaaga ccggcaagcc gcacgacctc gagctgttca agaccgtcgt cggcgtgaac
    14521 ctcatcggca cgttcaacgt gctgcgcctc ggtgcgtcgg cgatctcgaa gaccgatccg
    14581 ctggagcatg gtgaacgcgg cgtgatcgtc aacaccgcgt ccgtcgccgc attcgacggg
    14641 cagatcggcc agatcgcgta cgcagcatcc aaaggcggtg tcgccgcgat gacactgccc
    14701 gcagctcgcg acctcgcgcc cgccggcatc cgcgtcatca cgatcgcacc tggcatcttc
    14761 gacacgccga tgctcggtgc gctgcccgaa gagaagcgcg cggcgctggc ctccgacgtg
    14821 gtgttcccga agcgcctcgg tgatccgtcg gagtacggcg cactcgtccg cgcgatcgtc
    14881 gagaacggct atctcaacgg cgagacgatc cgcctcgacg gctcgctgcg catgccgccg
    14941 aagtagccag ctggccggtc agattctctg cggcgggtgc taggttcgcg gaatgcgccg
    15001 cctggccatg gttcccctcg tgctgttcgt cgtcgcttgc tccagcaaga agccggacgc
    15061 gcccgcgggt ggcagcgcgg cgccggtcag cgcggcgcgg cccgatgccg ccccagcagt
    15121 gaccgcggcg cgagacgcgc gcgcgtcgac acccgacgcg ccaggccgac cgagcgatga
    15181 cgcggtcgct ggtttcgggg tcggcgacga cggccgcggc ctgtattacg accggccgct
    15241 cgcgccggcg gatctcgacg gccgctcgct gcgcgacctg acgctcatgc gcaatgtcgt
    15301 ctatgcgcgc gccggtcacc cgtttcgcaa ggcgtggctg cgcgagtatt tcacggcgca
//
LOCUS       DATLBZ010000315         7297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4825021, whole genome shotgun sequence.
ACCESSION   DATLBZ010000315 DATLBZ010000000
VERSION     DATLBZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4825021"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VMZ53_16300"
     CDS             <1..327
                     /locus_tag="VMZ53_16300"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30070.1"
                     /translation="LFAIDSVPAAFAISEDTFIVYSSNVLAVLGLRALYIVLASALQG
                     LRYLHFGLAAIVGFAGVKMLIASWVKIPPLVSVLIIAVMMGLAILASIRANRVRPARA
                     IAQQGT"
     gene            complement(281..1345)
                     /gene="bioB"
                     /locus_tag="VMZ53_16305"
     CDS             complement(281..1345)
                     /gene="bioB"
                     /locus_tag="VMZ53_16305"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HUS30071.1"
                     /translation="MNWSALAERSLAGDIPTRDAARAVLHASPDETLALLDAAFRVRR
                     AYWGRRVSLHVLENAKMGACPEDCGFCSQSSKHASTSPSGEAPMKSVEDLVAGARRAH
                     ASRAKRYCMVTATRGPSQRDLDTICAAAQQIKSEMDIELCASLGILTEAKAKRLVDSG
                     VDRFNHNLETSESHYGKIVSTHTWRDRVETVQLAKAAGMDTCCGGIVGLGESEDDLLD
                     LAFALRDLDVDSVPVNFLDARPGTPLAGYPLVEPAYALRALCMFRFVHPRTDLRVAGG
                     REITLRALQAFALYPANSIFTQGYLTTGGAAVQADHQMIKDAGFELELASGEAVPADP
                     VAMANVTFPVARSLLPVVRD"
     gene            1417..2940
                     /locus_tag="VMZ53_16310"
     CDS             1417..2940
                     /locus_tag="VMZ53_16310"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUS30072.1"
                     /translation="MNKSLLLVAILAACNTPPARKPPSRGTPAKTDSAPKGEIVTAKV
                     LEMNSDKYGRPPIEFRPGSTSKIESPKPTKTAQGFQVEFGSSAQITTPTVYDHKVITS
                     GGFRSNQLYAFEAKTGNPVWALDLHDDGPSSPACERGVCVVNTESCTIFAIAADSGKL
                     LWSYYLGDPLTSAPTIAGQRVFAAYPAGGTADGKERPPGATHALAAFDLTSGKILWQL
                     WLDGDVMSAPVAAGEFVYVSTFGGTIIKVEQATGKVRYAMKSQVTSAPVVQFGSDGVE
                     QMYYTRGENWKDELGAYRKRESIIRADHNEPQTKYEAAAKDADYIDGKVQSQTVHAKK
                     AKEDDAANGFGGGAPASAAANYSFDGVGVGSVQSMQSFQGSRVLRFADKSLNTMGDEV
                     VATNADTGEKLWSYKLPGDTKTQGGFLGTAPLAAGNSVLLGTLDGKVLRLDPNKGTSI
                     AEYKVGSPIRSQPVVEDGWIYVGTEDGKLIAIDTKDKSITGWPTWGGNAQRTGVAIK"
     gene            3027..4004
                     /locus_tag="VMZ53_16315"
     CDS             3027..4004
                     /locus_tag="VMZ53_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30073.1"
                     /translation="MRRWFVVLLVGCGACSFEHGRLSGGDGDAQCTKPWWDASYAHRF
                     RVDTQAPAGYTLQIDATAVLAQSFTSSRDDVRVVVDDTIDIDRLITGSTLELMMPAEG
                     TVSIYTGNIAPPSPLADPSRVYRFAESFESFAVGDNVAARFDALPPSEWRVADDLGNH
                     IFHAQGAARHPAAIVGMSMTDGEIRARMRWGLGGTQQHNGLAARGSNMLPATMDGFVG
                     QLQGDSNRTRIAEYSDGFSPPAELTSQGFTVERGRWYELRLRFLGDDLALVIDGTPTL
                     ATTKTGADGTHVGLYAHDCDVDYDDVRVRMYQQPEPAYTLGPDEPRCAP"
     gene            complement(4005..4385)
                     /locus_tag="VMZ53_16320"
     CDS             complement(4005..4385)
                     /locus_tag="VMZ53_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30074.1"
                     /translation="MRGSLLLALALVAAVGCSKDKKGPSSKDGVIDAWKKGGLEPSAF
                     TEAKTDIGKNCSAGTVNKVDVLLCAFGSEKEAKDAEEKGLQWVGDTTGASQAQGEMLI
                     VAADRRKADPSGRTINQLLTLPAK"
     gene            complement(4385..5125)
                     /locus_tag="VMZ53_16325"
     CDS             complement(4385..5125)
                     /locus_tag="VMZ53_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30075.1"
                     /translation="MKLRILITFALLALFAATPALAGKKFGKQVRFAGIHPVPKGDGG
                     GICHIEGPHVHIYAANKLEYRMHDDDYVFVGDPVAYGWDGPKYAYKGNHPIQVNAVVS
                     GSPAVEYCYLNGPHYHYFEPEQPEFKLVGGAYFYVGEPPKAYIEARPQYVEINTRYEP
                     IVYTRPVVEVAPPQGWIGARVDIVGPAVVVDAPPPVVVVPARPVVEVHIPQPRLEVGI
                     GVHVGGGVIVGPGPARHGKYKHKKWKHR"
     gene            complement(5202..5573)
                     /locus_tag="VMZ53_16330"
     CDS             complement(5202..5573)
                     /locus_tag="VMZ53_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30076.1"
                     /translation="MYRLAVASLIALGLTGCHHAQTSPELRVLGVHQESHVFVQVTNP
                     AQRPLHLTKLDYVFASSKGTTIAEGSMPIERDIPGGAVAVLEVPLDADTSEPVTLRGK
                     LTGELDQIVRTFKLNAQIAAR"
     gene            5742..6218
                     /locus_tag="VMZ53_16335"
     CDS             5742..6218
                     /locus_tag="VMZ53_16335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HUS30077.1"
                     /translation="MKIEKGRRVTLKVDLSVAGGQQLEKSTVEFVVGSGKFLPALEDI
                     LMGLEKGAKKEGILKAKDAFGNPALHPLKKMKRSDFPKDAKLDVGEKLVAAGADGAHM
                     NVILKIEKVSGDDVDVRLVHPLAEKDLKYSLEVVNVSDPRPPAIPAAALELEPEES"
     gene            6220..6975
                     /locus_tag="VMZ53_16340"
     CDS             6220..6975
                     /locus_tag="VMZ53_16340"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HUS30078.1"
                     /translation="MHPRRLYAHRGASAERPENTIPAFQRALELGVDALELDVHLTRD
                     KVLIVSHDETAQRMTGAPLAWRDLDLVDAQRLDAGWGFVAKDGTRPFAGQGINVPTFE
                     TILREFPGVHLNVDLKGDDSVQAVLAMLRKHDAEDRVTLASFSTPTLVSVRRAGFPGE
                     TALAQAEVAALFTVPAVIWRQLPFTGSAAQVPTNAGPLRFDRAAFIAKCHAVGLRVDF
                     WTIDDVTDATRLLELGADGIMTNDPASLKGLFI"
     gene            7013..>7297
                     /locus_tag="VMZ53_16345"
     CDS             7013..>7297
                     /locus_tag="VMZ53_16345"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HUS30079.1"
                     /translation="MALKKGVGVKPAEGKLGILLPGMGAVATTFIAGVQAIRRGLGKP
                     IGSLTQLGHIRIGKRTDNTSPAIKDYVSLANLDDIVFGGWDIFPDNAYQAA"
BASE COUNT         1306 a         2389 c         2382 g         1220 t
ORIGIN      
        1 ctgttcgcga tcgactcggt gcccgcggca tttgcgatct ccgaggacac gttcatcgtc
       61 tacagctcga acgtgctcgc ggtgctcggc ctacgcgcgc tctacatcgt gctcgcgagt
      121 gcgctgcaag gcctgcgcta cctgcacttc ggtctcgctg cgatcgtcgg ctttgcaggc
      181 gtcaagatgc tgatcgcgag ctgggtgaag atcccgccgc tcgtctccgt cctgatcatc
      241 gccgtgatga tgggactcgc gatcctcgcg agcatccgcg ctaatcgcgt acgaccggca
      301 agagcgatcg cgcaacaggg aacgtgacgt tcgccatcgc gacgggatcc gccggaacgg
      361 cctcgcccga cgcgagctct agctcgaacc cggcgtcctt gatcatctga tggtcagcct
      421 gcaccgcggc gccgcccgtc gtcaggtagc cctgcgtgaa gatcgagttc gccggataca
      481 gcgcgaaggc ctgcagcgcg cgcagcgtga tctcgcggcc accggcgacg cgaagatccg
      541 tccgcggatg cacgaagcgg aacatgcata gcgcgcgcaa tgcgtaggcg ggctcgacga
      601 gcggatagcc cgcgagcggc gtgcccggac gcgcgtcgag gaagttgacc ggcaccgagt
      661 cgacgtcgag gtcgcgcagc gcgaacgcga gatcgagcaa gtcgtcttcg ctctcgccga
      721 gaccgacgat gccgccgcag caggtgtcca tgcccgcggc ctttgccagc tgcaccgtct
      781 cgacgcgatc gcgccacgtg tgcgtcgaca cgatcttgcc gtagtggctc tcgctcgtct
      841 cgaggttgtg attgaagcga tcgacgccgc tgtcgacgag ccgcttcgcc ttcgcctcgg
      901 tgaggatacc gagcgatgcg cacagctcga tgtccatctc gctcttgatc tgctgcgctg
      961 cggcgcaaat cgtatcgagg tcgcgctgcg atggaccgcg ggtcgccgtc accatgcagt
     1021 agcgcttggc acgcgatgcg tgcgcgcgac gtgcgccggc gacgaggtcc tcgacggact
     1081 tcatcggcgc ctcgccgctg ggggacgtag acgcatgctt gctcgattgc gagcagaatc
     1141 cgcagtcttc cgggcaggcg cccatcttcg cgttctcgag gacatggagc gagacgcggc
     1201 ggccccagta ggcgcggcgg acgcggaagg cggcgtcgag gagcgcgagt gtctcgtcgg
     1261 gcgaggcgtg gaggacagcg cgcgcggcgt cgcgggtcgg gatgtcgccg gccagactgc
     1321 gttcggcgag agcggaccag ttcatggccg cggaccctat cacgcaactt ggaacagatc
     1381 gcggcgtctt ctggtgcgtt gcaccccggg ggccccatga acaaatcact cctgctcgtc
     1441 gcaattctcg ccgcttgcaa cacgccgcca gcccgcaagc cgccgagccg cggtacgccc
     1501 gcaaagacgg acagcgctcc gaagggcgaa atcgtcacgg caaaagtgct cgagatgaac
     1561 agcgacaagt acgggcgccc gccgatcgag ttccggcccg gttcgacctc gaagatcgag
     1621 tcgccaaagc cgacgaagac cgcgcagggc tttcaggtcg agttcggcag ctccgcccag
     1681 atcacgacgc cgacggtcta cgatcacaag gtcatcacat cgggcggctt ccgctccaat
     1741 cagctctacg cgttcgaggc caagaccggc aatccggtgt gggcactcga tctgcacgac
     1801 gacggcccat cgagccccgc gtgcgagcgc ggcgtgtgcg tcgtgaacac cgagtcgtgc
     1861 acgatcttcg cgatcgcagc cgacagcggc aagctgctgt ggagctacta cctcggcgat
     1921 ccgctcacga gcgcaccgac gatcgcgggc cagcgcgtat tcgcggccta tcccgcaggc
     1981 ggcaccgccg acggcaaaga gcgaccgccc ggcgccacgc acgctctcgc ggcgttcgat
     2041 ctcacgagcg gcaagatcct ctggcagctc tggctcgacg gcgatgtgat gagcgcgccg
     2101 gttgccgcgg gggagttcgt ctacgtctcg acgttcggcg gcacgatcat caaggtcgag
     2161 caagcgacgg gcaaagtccg ctacgcgatg aagagccagg tcacgtcggc gccggtcgtc
     2221 cagttcggca gcgatggcgt cgagcagatg tactacacgc gtggcgagaa ctggaaagac
     2281 gagctcggcg catatcgcaa gcgcgagtcg atcatccgcg ccgatcacaa cgagccgcaa
     2341 acgaagtacg aggccgctgc gaaggacgcc gattacatcg acggcaaggt gcagtcgcaa
     2401 accgtccacg cgaagaaagc gaaagaggac gacgccgcga atggcttcgg cggtggcgcg
     2461 cccgcatccg ctgctgcgaa ctattcattc gacggcgtcg gtgtcggcag cgtgcagtcg
     2521 atgcaatcgt tccaaggctc gcgcgtgctt cgcttcgcgg acaagagcct caacacgatg
     2581 ggcgatgaag tcgtcgcgac gaacgccgac accggcgaga agctctggtc gtacaagctg
     2641 cccggcgaca cgaagacgca aggcggcttc ctgggcaccg ctccgctcgc ggccggcaac
     2701 tccgtgctgc tcggaacgct cgacggcaag gtgctgcgcc tcgatccgaa caagggcaca
     2761 tcgatcgccg agtacaaagt cggatcgccg attcgctcgc agcccgtcgt cgaggatggc
     2821 tggatctacg tcggcacgga agacggcaag ctcatcgcga tcgacacgaa ggacaagtcg
     2881 atcaccggct ggcccacgtg gggcggcaac gcgcagcgca ccggcgtcgc gatcaagtaa
     2941 ggcgaaacgc gtcgatcacg gggctggccc acgtgcgcgg caatgcgcag cgcaccggca
     3001 tcgctgtgcg gtgataccct tgtcgggtgc ggcgttggtt cgttgtgctg ctcgttggct
     3061 gcggggcatg cagcttcgaa cacggacggc tgagcggcgg cgacggcgac gcgcagtgca
     3121 ccaagccgtg gtgggacgcg agctacgcgc accggtttcg cgtggatacg caggcgcctg
     3181 ccggctacac gctgcagatc gacgcgacgg cggtccttgc gcagtcgttc acgagcagcc
     3241 gcgatgatgt gcgcgtcgtc gtcgatgaca cgatcgatat cgatcggctg atcacgggct
     3301 cgactctcga gctgatgatg cccgccgaag gcaccgtgtc gatctacacc ggcaacatcg
     3361 cgccgccgtc gccactcgcc gatccatcac gcgtctatcg attcgccgag tcattcgaaa
     3421 gcttcgccgt tggcgataac gtcgctgcgc gcttcgatgc gctgccaccg tcggagtggc
     3481 gcgtcgctga cgatctcggc aatcacatct ttcacgcgca gggcgcggca cgccatcccg
     3541 cggcgattgt cggtatgagc atgaccgacg gcgagattcg cgctcgcatg cgctgggggc
     3601 ttggcggcac gcagcagcac aacggcctcg cagcgcgcgg cagcaacatg cttccagcga
     3661 cgatggatgg cttcgtcggt cagctgcagg gcgactcgaa ccgcacgcgg atcgccgagt
     3721 actccgacgg cttctcgccg ccggccgagc tcacgagcca ggggtttacc gtcgagcgcg
     3781 ggcgctggta cgagctgcgc ctgcgcttcc tcggcgacga cctcgcgctc gttatcgacg
     3841 gcacaccaac actcgcgacg accaagaccg gcgccgatgg cactcacgtc ggcctctacg
     3901 cccacgactg cgacgtcgat tacgacgacg tgcgcgtccg catgtaccag cagccggagc
     3961 cggcgtacac gctcggtccc gacgagccgc gctgcgcgcc gtaactactt cgccggcaac
     4021 gtgagcagct gattgatcgt gcggccgctc ggatcggcct tgcgtcgatc ggcggcgacg
     4081 atgagcatct cgccctgcgc ctgcgatgcg ccggtcgtgt caccgaccca ctgcaggccc
     4141 ttctcctcgg cgtccttggc ttccttctcg ctgccgaacg cgcagagcag gacgtcgacc
     4201 ttgttgacgg tgcccgcgga gcaattcttc ccgatgtccg tcttggcttc ggtgaacgcc
     4261 gacggctcga gcccgccctt tttccacgcg tcgatcacgc cgtctttcga cgacgggcct
     4321 ttcttgtcct tgctgcagcc aacggccgca acaagcgcga gcgcgagcaa gagcgagccg
     4381 cgcattagcg atgcttccac ttcttgtgct tgtacttgcc gtggcgcgcc gggcccgggc
     4441 caacgatcac gccaccaccg acgtgcacgc cgatgccgac ttcgagccgc ggctgcggga
     4501 tgtgaacctc gacgacgggg cgcgccggga cgacgacgac gggcggtgga gcatcgacga
     4561 cgaccgctgg ccccacgata tcgacgcgtg cgccgatcca gccttgcggc ggtgcgactt
     4621 cgacgaccgg gcgcgtgtag acgatcggct cgtagcgcgt gttgatctcg acgtactgtg
     4681 ggcgcgcctc gatatacgcc ttcggcggct caccgacgta gaagtacgcg ccaccgacga
     4741 gcttgaactc cggctgctcg ggctcgaagt agtgatagtg cgggccgttg aggtagcagt
     4801 actcgacggc aggcgagccg ctcacgaccg cgttgacttg gatcgggtgg ttgcccttgt
     4861 acgcgtactt cgggccgtcc cagccgtacg ccaccgggtc gccgacgaag acgtagtcat
     4921 cgtcgtgcat gcggtactcg agcttgttcg ccgcgtagat gtgaacgtgc ggcccctcga
     4981 tatggcagat gcctccgccg tcgcctttgg gcaccggatg gatgccggcg aagcggacct
     5041 gcttcccgaa ctttttacct gctagcgcag gcgtcgccgc aaagagcgcg agcaacgcaa
     5101 aggtaatgag aatgcgcagt ttcataggac ctcctgacgg agcccctatt gcgccgcgct
     5161 cccgccggag aatcaaggca cggtgtcgcc ggatgtgcga ttcagcgagc ggcgatctgc
     5221 gcgtttagct tgaatgtgcg gacgatctga tcgagctcac ccgtgagctt gccacgtagt
     5281 gtgacgggct cactggtgtc cgcatcgaga ggaacttcga gcaccgcgac cgcgccgcca
     5341 gggatgtcgc gctcgattgg catcgagcct tccgcgatcg tcgtgccctt cgatgacgcg
     5401 aacacgtagt cgagcttcgt caggtgcagc gggcgttgcg ctggattcgt gacctgcacg
     5461 aagacgtggc tctcttggtg aacgccgagg acgcgcagct ccggagacgt ctgagcgtga
     5521 tggcaaccgg taagtcccag tgcgataagt gaagcaacgg caaggcgata catcgaaagc
     5581 agaaacttat tgtgcggctt ttgtgccaag gggtccaaaa aacgcaacta cgcgtcgtcg
     5641 ctggcgtacg ggcgcccgcc ctgtcggcga tccacacagc cgttcacaga tccgtgcccg
     5701 cgctgtcgca gtcgacgaca gagatccgtt aacgtcgcaa ggtgaagatc gagaagggcc
     5761 gtcgcgtaac gctcaaggtc gatttgtccg tggccggcgg gcagcagctc gaaaagagca
     5821 ccgtcgagtt tgtcgttgga agtggcaaat tcctgcccgc gctcgaggac atcctcatgg
     5881 gcctcgagaa gggcgcgaaa aaggaaggca tcctcaaggc gaaggacgct ttcggtaacc
     5941 ccgcgctcca cccgctgaag aaaatgaagc ggagcgactt tccgaaagac gcgaagctcg
     6001 acgtcggcga gaagctcgtc gccgccggtg cagatggcgc gcacatgaac gtcatcctca
     6061 agatcgagaa ggtgtcgggc gacgacgtcg acgttcgcct cgtgcatccg ctcgccgaga
     6121 aggatctcaa gtactcgctc gaggtcgtga acgtcagcga cccacgcccg ccggcgattc
     6181 ctgcggccgc tctcgagctc gagcccgaag agagctgaga tgcatccgcg gaggctctac
     6241 gcacatcgcg gcgcctccgc cgagcgcccg gagaatacga tcccggcgtt tcagcgcgcg
     6301 ctcgagcttg gcgtcgatgc gctcgagctc gacgtgcatc tcactcgcga caaggtgctc
     6361 atcgtctcgc acgacgagac ggcgcagcgc atgaccggcg ctccgctcgc atggcgcgat
     6421 ctcgatctcg tcgatgcgca gcgtctcgat gcaggttggg gcttcgtcgc aaaagacggc
     6481 acgcggccgt tcgcaggaca aggcatcaac gtgccgacgt tcgagacgat cttgcgcgag
     6541 ttcccgggcg tgcatctcaa cgtcgatctc aaaggcgacg attcagtgca ggcggtcctc
     6601 gcgatgctgc gcaaacacga cgccgaagat cgcgtaacgc tcgcgagctt cagcacgcca
     6661 acgctcgtct ccgttcggcg tgcgggattt cccggcgaga cggcgctcgc acaagcagaa
     6721 gtcgctgcgc tgttcacggt tcccgccgtg atctggcgac agctgccttt caccggcagc
     6781 gcggcgcagg tgccgacgaa tgccggaccg ttgcgcttcg atcgcgcggc gttcatcgcg
     6841 aagtgccacg cggttggact gcgcgtcgac ttctggacga tcgacgacgt gaccgacgcc
     6901 acgcggctcc tcgagctcgg cgccgacggc atcatgacga acgatccggc aagcctcaaa
     6961 ggcctgttca tctagccttg aaacagacgc ttccatgggg caggttgcgc ccatggctct
     7021 caagaaaggc gtcggcgtca agccggctga aggcaagctc ggcatcctgc tccctggtat
     7081 gggcgcggtt gccaccacgt tcatcgccgg tgtgcaggcg atccggcgtg ggctcggcaa
     7141 gccgatcggc tcgctgacgc agctcggtca catccgcatc ggcaagcgga ccgacaacac
     7201 gtcgccggcc atcaaggact acgtctcgct cgcgaacctc gatgacatcg tgttcggcgg
     7261 ctgggacatc ttcccggaca acgcgtatca agccgcg
//
LOCUS       DATLBZ010000316        41727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6140329, whole genome shotgun sequence.
ACCESSION   DATLBZ010000316 DATLBZ010000000
VERSION     DATLBZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 41727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41727
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6140329"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1768)
                     /locus_tag="VMZ53_16350"
     CDS             complement(<1..1768)
                     /locus_tag="VMZ53_16350"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUS30080.1"
                     /translation="MRALPCASALDRPTLLDHIPELLDRIAQLAVDPSRVASEREACA
                     HHALARLHEGFDLSEVISEFRVLRQAILQALSDPSVGFLQASELRLVDDAIDEAIAES
                     VKQYTATRDRTLLGLDRVATAALQSESLDELLGRLLEVLRETTPAIDTAAIYLRDGDV
                     LHLRAGVGVERDVEVGVVIQIGDGFAGQVAARNEPLTLQNPRPEDIRSPALARASLRV
                     LYGVPIVDGGVVIGVAKIGSINAPEFSEADQRVFSAMVARATAAIIQHVLRDQARAAA
                     IKLAQRDEQFRALADNMAQLAWMADPTGSIHWYNRRWYDYTGTTFEEMQGWGWEKVHH
                     PDHLQRVVTRWKRAIDRGEPWEDTFPLRGHDGFYRWFLSRAMPIRDEAGAILHWFGTN
                     TDVTLRRFLDQTTKLLARSLDYRELLRDLAASVIPDFADCCVIDVLEGDKVRHVAYAH
                     VDPTKQELLAKFVAGHEPQTTDANGSWQVLRANEPILIPDVPDTMLSSPDFTALGFKS
                     WIGAPLLARGRAVGVIHLMTSDSGRRYTERDLEVAVELGHRAGGAVDNTRLYAQAQAA
                     LGVRDDILAIVSHDLRTPLSSLD"
     gene            1959..2093
                     /locus_tag="VMZ53_16355"
     CDS             1959..2093
                     /locus_tag="VMZ53_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30081.1"
                     /translation="MDLWSAAERIRDARVFLILFDPAAPACQTHYKNHPNGTLVEDRD
                     "
     gene            2168..3730
                     /locus_tag="VMZ53_16360"
     CDS             2168..3730
                     /locus_tag="VMZ53_16360"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS30082.1"
                     /translation="MSRHAQAVEVVAMLGDSVVGVKHCCDPNANGKVQRATYTLFAVA
                     AVAMLVAGVAFWSAVETAAYNQSRYEYETTVLGKPRYSVRTRSLPRGCESIAFGALLA
                     SLVACTAAVLRMRREREGSTVRIGTAPGVDFPVAGAPAEDFALVVTQSDHFAVQRGDE
                     LVPVMDDFAARLCFGGATFLVCAIERPRRVPVPLFELDAEFSRILVGTAAMFAVFMLV
                     VFQLPIEAESASIEISETEETFVSVDWPAPESAPSPYDGVDGDSGGSGTSMALDPGRM
                     GNVESQRADGQYKIEGRIDPQLARQQAIEQARYAGILGSTSVTSGNAFVSLTGSFDAS
                     RGFDDTNIYGGKLGDEAGEMAGGFGYARSKFGPGGGGTGWGTICAGDFGTIGHGSGTG
                     EGYGIGGGRCGTTCRGHVAATPSVSIGQPVAIGALDKAIIRRYIKRNIDKIRYCYEKT
                     LLAKPGLSGTVSTQFFIAPDGHVSSAAAEGVDVEVASCVADVIEGISFPRPSEGAGVQ
                     VSYPFTFERAGE"
     gene            3826..4887
                     /locus_tag="VMZ53_16365"
     CDS             3826..4887
                     /locus_tag="VMZ53_16365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUS30083.1"
                     /translation="MAGLLHPSFGGMNKRTLLADLCDRTGATELALAVRKRATLPWLP
                     VVTFHRIANVSRQYPFDEEVVEATPAQFERQLAIVRQHFTPVTTADVVKALDGQPLPA
                     NAVFVTFDDGYLDNHAVALPLLQKYGVKATFFIATDYVTRRRLFWWDRIAFAVKAAAR
                     PKLALSYPRPMVLEVGTPALRRAATKTLLRLVKSTYELDLERFLDELTAAAEVAWNTD
                     VERELADGLLMTWDHVRALKAAGMDVQSHTCTHRVLQTLSSEHALAELVDSRAALERE
                     LGERIFAVSYPVGHTINDRIDVRKAVARAGYRVGFTNATGVQPMWDVDRYNFKRISLP
                     PQLPDSLFRAMLTLPMVFE"
     gene            complement(4888..6207)
                     /locus_tag="VMZ53_16370"
     CDS             complement(4888..6207)
                     /locus_tag="VMZ53_16370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUS30084.1"
                     /translation="MHGFIAQLIDVESESLPILREALAEAGCLDIDPAATVGGVLLER
                     GEPQQQRRLVIISIHRGNIGGLQRLAGGSQPIQVLALCDEADIELAFGSGATQVVTRP
                     LRRRELIGRIRESMRNRSETDRRESRERHMCDAIVALQKEKQDLERLVCVDALTGIAN
                     RRHAMELLANEWKRAARDHAPLALIMIDLDCFHAYNEQYGHLGGDACLQRAADAMVRC
                     LRRPSDFLGRYGGEEFMAVLPNTDAVGAKIVAERLRAAVEGLRMPHAASTCSEVVTVT
                     VGFASIRVLADDQMERLIAAADGALLQAKAHGRNAVGGIAPLVRPTRVSAQLWERYEP
                     VYVDPWYADRVPAFLDTTKAQVSALADSVRNGERRSGLTMRRLRNSAHELGLTTIERL
                     ALNVEDALREGELVQLRTATEELLQYVMHVQIIYRRTEDAHAASSVA"
     gene            6598..7014
                     /locus_tag="VMZ53_16375"
     CDS             6598..7014
                     /locus_tag="VMZ53_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS30085.1"
                     /translation="MDSIPLHVPSARIGTDPGEEYRCMLIIESDDVLRRTTTRQFQAL
                     KWNVISAHDLKSGLELAIRYQPSVIISDLALPDAAGYGFARTLRTLIDHDIRVVGMTT
                     DPENLCAEVRAAGFDLVFAKPLDVAQTHEQLGRKAK"
     gene            7011..7853
                     /locus_tag="VMZ53_16380"
     CDS             7011..7853
                     /locus_tag="VMZ53_16380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS30086.1"
                     /translation="MSDDNEPEQHPAPAPATTPTFVHSKPLLDPVLREEAEFANRDYA
                     AHANDLELSDEMFAKYSRPAELWDWRQLAAMMLGNVKGKALLDYGCGMGEESVYWAKL
                     GAQVTAIDISEVGVSTTKRRAEYHKLDIHAFEMRCDPTSFPAASFDRIHGMGILHHVG
                     IDNALAEVWRLLRPGGIAVFLEPMGDHPGIEAVKEFLMRRARFLGDFDEVTDHEHNLR
                     WAEIEEATKRFARYSAVPFHLLYRLKRFAPYKLHTAMRRIDAAMLTLAPSLKRYCGAV
                     AIQV"
     gene            complement(7911..8726)
                     /locus_tag="VMZ53_16385"
     CDS             complement(7911..8726)
                     /locus_tag="VMZ53_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30087.1"
                     /translation="MRKILLTGFALALAACNDNARPEDELPDNPVDPVDPTDPEDPPN
                     PQETARDYDELAAILSAHLRAEFSVQLTGAAIVRNEAVLPEGFAITGDDGVDALGTGT
                     LNGLTINFGLHCNDGSAAHIRVPCDANAHHGHLKLNISGTQTMGTISMNDFQRAVNWE
                     IRDVNLGKARFRGPDGTVLKSQVVTNSEPADYVVMFEALYEQVRFLPDTTIPTFGTID
                     FKVNTQRTRNGDRRVFDSQAHLTYGASGVPTTLTFDGGQSYALDLKTGEVIKQ"
     gene            8987..9352
                     /locus_tag="VMZ53_16390"
     CDS             8987..9352
                     /locus_tag="VMZ53_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30088.1"
                     /translation="MTIRALGWFLVGVCALAACGKDSKGGPSVADLEKRCDALSKACA
                     DTDKHVEKLGGECKAAAKTESERGCGDKAIALYNCYENAVCGTGDRVWALPDLKVLAE
                     RHKKCDAEQAALTACVDKK"
     gene            9355..10260
                     /locus_tag="VMZ53_16395"
     CDS             9355..10260
                     /locus_tag="VMZ53_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30089.1"
                     /translation="MMKIGFAGLVVVALCAVAKADAFECKPTETQAECHTRLKCKANE
                     EVEDCQKRLRAAAFSGGQSSGGDNRGGDDRGGDRGNDRGGDDRGNDRGNDRGNDRGGD
                     DRGNDRGGDDRGNDRGGDDRRGSRRSSRRRGGGGGGGGGGGGGHGFEANKTFGLGLEL
                     GEPAGLNGKYFFGPSTALDFGVGWIYRHYYYGDGLHLYGDILFHPVSLASTPALEIPL
                     YVGPGLRYWDFQYCVGGICDYGGSAIGIRVPIGISFDFNNVPLDIFVQFVPTIDFIRG
                     DYYDRYRDRNHFGVDFSVGLRFWFK"
     gene            complement(10261..11325)
                     /locus_tag="VMZ53_16400"
     CDS             complement(10261..11325)
                     /locus_tag="VMZ53_16400"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HUS30090.1"
                     /translation="MRVFGIVSSVIFAAACNSSHPGGGGGGGGGTGPDGGTTTTPDAR
                     ADDAASAPDAATAKLSTSSIVLAAALDGQPATDLYVLDGSTTTRLTQTAGGELYPSIS
                     PDRTKIAFVRDFKLFVADANGRNEQVVAMTVGRQRKINDFVYSTTLGPAAWSPDGTQL
                     AYPYPREPFIVNDGEDMIDESYGTTLHVVNADGTGDHLVSSAINGTVNSLGWSGAVIS
                     LSMAYDCPDCAGGEVVGVIKPDGTGYHEFDFRAAVQPGIEVGSPNKHLDWSPNGAKWV
                     FVISSQYGNYDAPGKIFTSTASVDDETPLVAAQGWSPRWSPDGTEIAFIGEQGIYIVA
                     AGGGSPRHVLASTNLRGLDW"
     gene            complement(11371..11838)
                     /locus_tag="VMZ53_16405"
     CDS             complement(11371..11838)
                     /locus_tag="VMZ53_16405"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUS30091.1"
                     /translation="MAEHIAIIRWQRAGTPEDFLKGRYSREHTWTFDGGLTVPGSPAP
                     GNVPAAFANPAAVDPEEAFVAAISSCHFLTFVWLAFKAGFVVDSYEDRAVGRMAKNER
                     GVAWVSETTLHPSITYGGTKQPSREEEARLHHDAHDQCFIAQSVKTNITVAGF"
     gene            complement(11840..12478)
                     /gene="yfcF"
                     /locus_tag="VMZ53_16410"
     CDS             complement(11840..12478)
                     /gene="yfcF"
                     /locus_tag="VMZ53_16410"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF011693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HUS30092.1"
                     /translation="MSELTLYGDAAWESPWVFHAMVALDELKVKYTLEPIRRPIADDL
                     KGELKTKALLPFVPALVHGDFWLTESSAISEYLAEQFAPPSYPRILPADARERARARQ
                     VISWLRTSIMGLRNDRPTTSVFMKPVTNPLSEKGRADADELVRVASQLVAPGKQSIAS
                     EWCIADADLALALMRLVGNNDSFVPKHLVDYALATWGRRSVLKYLSYIPTTH"
     gene            12537..13718
                     /locus_tag="VMZ53_16415"
     CDS             12537..13718
                     /locus_tag="VMZ53_16415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30093.1"
                     /translation="MEDRFGPYSVFECLGAGGMATVHRASIELEDDETREVALKRLLP
                     QLAADKRFIDDFIREGKLAAQLKHPNIVRILELGRIGKTYFIAMDLVRGRPLMSLMRK
                     AYQKRLPAPIGVVLGLMRELCDALEHAHDAQIVHRDLTPSNLIVTDDGHLKIIDFGVA
                     KALVGNLQTSSGLAKGKLGYMSLEAIGGKQLDTRADLFSTGVVMWELLATKRLFKGTN
                     EIEIIQQIRDGDVQPPSVYNKDCPRDLDDIVLKALQRDKEERWSSAGEMRDALDEIRR
                     FHRGDTSANAVVNWMTELRGETVRGPRLESDGESTQVGLETEDMFEGDGTSGPREVGS
                     DALAFGSQRGAQPVYARGSDVQTYDDTMDEPESTEVADTTGPKFKEVDTIVSTLHPRK
                     S"
     gene            13751..14302
                     /locus_tag="VMZ53_16420"
     CDS             13751..14302
                     /locus_tag="VMZ53_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30094.1"
                     /translation="MYADAIKVREAREQYFIANGFSDATYREDWARIKLGPVPVVFPN
                     TKSRKEALPIHDLHHIATGYSTTIEGEGEIAAFEIGGGCGRYAAAWLINAGGFALGLV
                     TNPRKIFRAFVRGRHSRNLYREGFRDSLLDLSVAELRKRVGTDTATPVATGQDKLAFV
                     AWCFAVSWPVSLAMLAVGVVLAR"
     gene            14437..15843
                     /locus_tag="VMZ53_16425"
     CDS             14437..15843
                     /locus_tag="VMZ53_16425"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS30095.1"
                     /translation="MVALVGATGAATGALMLHRSNAATRPTAPQLDGPVACGTPAPAH
                     ADAKLTHGKLGGALSAGKLLRNGDGEAFAAFELTTDNVAGQRPPLDLAIVIDHSGSMS
                     DGRLEHAKSAAVGIVGKLGAQDHVALIQYDDTAQVVFQSAAMDARGKESLMSAIRGLD
                     LGGGTNLHAGMLLGRSEVERAHADGQVNRVILLSDGRANSGVVDTPHIADAARDAANH
                     GVRITSVGIGLDFNEDLMEAIAEAGRGNYHYVKEASDLDRVIAEEMAGIQTTVATNVE
                     LRLTPACDGVQIAEVHGYESKREGTAFVIPMADLAASDSRKLLVSLKVPANQNGQVAT
                     LHAELVYRDAKGTEVHKAKLDLGLEVSEDANAVTASIDSNVMAQALQLQAAKSMREAA
                     QAYEQGDQAAAVRIIDQSTRDISEKSAHYKIEAKTAGTRASLGEMQKKAAEYKPDSYE
                     GKDMLKASKSKARVMSKK"
     gene            complement(15827..16057)
                     /locus_tag="VMZ53_16430"
     CDS             complement(15827..16057)
                     /locus_tag="VMZ53_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30096.1"
                     /translation="MGPVPSLSVSALQELRRIYCKHLLEVFASSETQELYERGCISGR
                     FAEGDGRRAFIVEEVTPLGVAVLDKLEATSST"
     gene            complement(16109..16864)
                     /locus_tag="VMZ53_16435"
     CDS             complement(16109..16864)
                     /locus_tag="VMZ53_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30097.1"
                     /translation="MSKLSQLVVFTSLFASSIAVAQPGATPPIDESVDMSSPLPASNT
                     RAPGETDVVEREPEVAPNVYVRKPVRDPLATTHVWGFGVRATGLSGIGALPGVNYGGE
                     LAAQVRHQEIFAELAVGRWKPEHTYVVAESPDHVELGLNVWTVRVGWSSMKTPLRGWV
                     LSEAGELASARQMAGVVPRMMTGSTPMDRRWKAVGGGFGVAWGMTDTARLVGSIELAI
                     PVNRDDVNLDGYGTYEPDPISARSCVGLEVGWR"
     gene            complement(17057..17413)
                     /locus_tag="VMZ53_16440"
     CDS             complement(17057..17413)
                     /locus_tag="VMZ53_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30098.1"
                     /translation="MAVYKLERFEVRPEARDEAERAMHAFAMYVREHLDDSMWTTYRD
                     TSAPNRYIAMTRTNDVAADQRHADADGTKAFVATLSALVIGDVESAEYELVTSSDLAP
                     RHRDRGGARGRRRLGR"
     gene            complement(17427..18170)
                     /locus_tag="VMZ53_16445"
     CDS             complement(17427..18170)
                     /locus_tag="VMZ53_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS30099.1"
                     /translation="MKSLSLVVCLLAACGGGNNGGNNGDDTNDTPDAKVFMDAPVVAP
                     SMFTISGDVTEQGISGESPVSGVSIALYSNAAQQTPIAMATSDAQGKYTMTVMTNGTA
                     IDGFVLAKKSGYVDLYLYPTAPFSKDTDGDLNMLTPSNRDFLSNLASGNQMSGKGLIG
                     LAIIDANGMPVAGATISAMPAAGAYRYMGSNGYPSGSAMSTNTDGVAFMFNVPSGPIT
                     ISATKAGMTFHSHVVEARADKMTTTAIAP"
     gene            18276..19625
                     /locus_tag="VMZ53_16450"
     CDS             18276..19625
                     /locus_tag="VMZ53_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30100.1"
                     /translation="MVDDPAVRSVRVAAALCDRFAELLKGPTIVTAHKGRGALIGIKG
                     NEDDLSLRLGEAQQIYPEIWSHLDEARKAFGARGVALPAFDALRETERQFGGTGAGVD
                     MKFTQYGDPTYGMQETVKKANFNQAGLARARSAIQAITLATPDIDWASIEKAEANDPH
                     AKAFRRATIIKRIWMFGLLIAVIGIPFWWLLWSRHKENEKREKWRRQYEQTSYQPPAP
                     VLPPTPPADLLPAAEQAELANQAAALDFKLAESLTNWEQLTAPAVLAKLKAGSEPCAY
                     PVTPPPKDAGDDFIKTGDNDTKVFAPSDFMGYLAKDGVVPSDELYEYQRNLKQVLALF
                     EKGAATARTKERWAVITEPFMFVLIDRSDEPEILDMTSTPISFKPGRVTGRAYVFSIK
                     DGKMVCTGAIDAKSSPPAAAAAYLDGVTSESVAGKVLHRELEVRIREGLANLKAVAP"
     gene            19680..19988
                     /locus_tag="VMZ53_16455"
     CDS             19680..19988
                     /locus_tag="VMZ53_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30101.1"
                     /translation="MAKDDVDNLARMFKALGNPNRLRLFLNLLEESKLDLAKGRIHDC
                     FLVKLLGGLGDIGAPTVSHHVKELSDAGLIQTQREGKQLICSVDPEGLEQLRQALANG
                     "
     gene            20080..21093
                     /locus_tag="VMZ53_16460"
     CDS             20080..21093
                     /locus_tag="VMZ53_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUS30102.1"
                     /translation="MTYQQIAIEKFGSASTLHVSQLAPRAPGADEVSIDVAYSGVNFA
                     DVQMRLGFYPDAPKKPFVPGYEVSGTVSAVGANVKDIKVGDAVVAGTYFGGYASHVTI
                     PARQVFKLPKTVDLEAGAALPVNYFTAQLALFEMARVRAGDRVLIECATGGVGVLAIQ
                     MARHAGAEVTGLTTSSHKKGFIENLGAKAYTREEFRANETLRGYDFILNASGGAEINW
                     QRARLGMTGRMVCIGLSSGVKDGKRNFVRIATAALRTPRISVLKLFDANTGIYALNAL
                     HVLQDETWVEKLTKSMVGVEQMNLKPHVGKVFPAAEVGAAHAFLETKQATGKVLLHWN
                     SAN"
     gene            complement(21097..21945)
                     /locus_tag="VMZ53_16465"
     CDS             complement(21097..21945)
                     /locus_tag="VMZ53_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30103.1"
                     /translation="MRKAGLLVSVFVFGLVACGPNDRDDMGGDDTGDPGGGGGGTGSG
                     SGSGSDGTIVYVYAHSASTLYRVDPDTLAVQMVAPFGWGSVGSDSMTDIAIDKAGVMI
                     GISYNRVYKIDPATAATTLLSNSLNGTFNGLSFVPASMLGQTGDDILVGTNNSDGKVM
                     RIDPMTGSATQVGDMGGFQSSGDLVAVEGFGTVQTVVNGPFSGADKLARLAPNTFAAS
                     AIGGGTGYSQIWGVAFFKGKVYGFTNGGQFVLIDPSNGGAMMVTQTPGISWWGAGVTT
                     LAPVLQ"
     gene            complement(22054..22815)
                     /locus_tag="VMZ53_16470"
     CDS             complement(22054..22815)
                     /locus_tag="VMZ53_16470"
                     /inference="COORDINATES: protein motif:HMM:NF042890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6748 domain-containing protein"
                     /protein_id="HUS30104.1"
                     /translation="MAISIISGGIAGCASTGVDDELAGETQEDAALDGKADGAVDGSY
                     TYFEIWRDMRKCVSPLCGGFFLQRVNRTTTVCHNGTSKWSCYTPELDWSEAKLSEELQ
                     GALLAKAQEDATTPGAVALVRGRFAPKTYASYGNMGRFVVTEAWVAEGEGVSEGVFAR
                     VHDNGVRCIQAPCPTVTEKALNTSRWANIAGLDWDAGGFEPDQVAKMTQEMVDRPSGV
                     IIAGERYSFKENNVSAKGRTVTAAYRRLESPVAEH"
     gene            complement(23048..23962)
                     /locus_tag="VMZ53_16475"
     CDS             complement(23048..23962)
                     /locus_tag="VMZ53_16475"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HUS30105.1"
                     /translation="MSSSAIIVHGGAGVVPDDRHAQLREGCRRAAEVGDAILVAGGSA
                     LDAVVAAVRVLEDDPEYNAGTGSALTREGRVETDASIMDGATQRVGAVAAVPDLGNAI
                     ALARAVLDANEHVILAGSAAWKFASSMHIRPAPTGTLVTERARERLAAFLEGRAKETS
                     GGTVGAVARDQRGRFAAATSTGGIVGKRTGRVGDSPIPGAGTWADTRCALSATGDGEA
                     ILRVALTRSIALRCELTGASLRDATIESLLELARITGLSAGVIGVDARGVIGLQLTAT
                     MPIASIIRGDSTGTTADSMGEQVEIRSD"
     gene            complement(23993..24874)
                     /locus_tag="VMZ53_16480"
     CDS             complement(23993..24874)
                     /locus_tag="VMZ53_16480"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUS30106.1"
                     /translation="MIRIALASLLPFVVACSSTPGPTKPGTSTAPLSLAKDPHMISIP
                     AGRFIAGSTPEERASAYDDYLATSGHDTARENKWFDAEEDRHQTGIDAFRIDLMPVTQ
                     AEYAEFVLAKRAPPPSIDEQAWTAQGFVQDFATVQRFNWKDGGPPIGREDHPVVLVTW
                     KEAEAYCKWRGELTGHARRLPTADEFEKATRGEGGLVYPWGNVYEPTKLNSAVKGPGD
                     TTPVGQYTDGASPYGVLDMAGNVFQWTSTPFREGHMTVKGSAWEDYGGIGRGASRHGR
                     PVDARHVIVGFRCAASE"
     gene            complement(24871..25701)
                     /locus_tag="VMZ53_16485"
     CDS             complement(24871..25701)
                     /locus_tag="VMZ53_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30107.1"
                     /translation="MFASSRDRSLDTTSLWIARLVADAVPARLTAGDAIDSHPTWSRD
                     GRSIVFASTRDGGDFDLYRLAIADGRASGEPQPLTRGDGHEITPTIASDGTIVYASVT
                     PKADGSIDSHLEARAPDGTITSLTAGPADASPQLSPDDRTIAFSRPQEHEAGLDGELW
                     TMPVHSDAALRVIDLPLTDESGPVWSPDGRYLFATSILRGANGNAVFSSVIVIDTHAS
                     PRKARLLADRSGAVARLTPAIARRDLDSAALASDPEYLPELARIMAAAMATQSPSSSP
                     "
     gene            complement(25931..26212)
                     /locus_tag="VMZ53_16490"
     CDS             complement(25931..26212)
                     /locus_tag="VMZ53_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30108.1"
                     /translation="MMDFSSWPRDQEQTFVVYEQYGQESQRKAWMGGLIAGIAFLVLA
                     LGVYAGVEPDHKDMTKGMNMDNLTKKSKKTEAPAPAPAPTPAPAPTPAQ"
     gene            26523..27359
                     /locus_tag="VMZ53_16495"
     CDS             26523..27359
                     /locus_tag="VMZ53_16495"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HUS30109.1"
                     /translation="MKDAGIFGDLDDKVQLSLPAGLTAERVSATVDRKHKLLVLSIDD
                     FPRKVYPLTGTETLTVGSIELALREGDRDELKPVLTAERTKEAAPARDRDKDGIPDPL
                     DILIGAKKTVLNADAYTEKAQSYITLKYPGGDVDRSMGVCTDVIIRSVRNAGLDLQKE
                     LHEDILKRGKAAFPMIRKAADASIDQRRVGTLLPYFKKHWEQHTAKLDDPSDPLRPGD
                     IILMDTFPSRSGPDHIGIISDQLDENGLPLVINNWTNGTVTSEMDLLEFVPVLYRFRL
                     PA"
     gene            27392..28957
                     /locus_tag="VMZ53_16500"
     CDS             27392..28957
                     /locus_tag="VMZ53_16500"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS30110.1"
                     /translation="MKRALLAVLLVLTSMLAGPSRALADDEAAKSLSYRAVVDRVELQ
                     PSPLGGQQLRVFLSALEIGGKQLELFDPQYKLKLYVGSGEKKVPYSLGSYASTDTDTA
                     LVVLVQASLDYAEALPAISDALDAALLPALSTHTQVVVLPFGETAATGKLGSVKQAIG
                     KGLSTDNSAGDPALLDAIDRALQIVRKAKTEPEGRPLRKMIIVIGDGRDMSGDKERVT
                     RTGTRAAKDGVRIHCIAFSSADIRRPMLALGELSKRSLGTFRWVRTSSGESWKAALEQ
                     LRDEINKQYVVTYFVSPEDDVAGHKMHIVIAGRIDATSNELKIPETPACGANECKADY
                     CSADKCVRYQSDGGRGILGWLLLIGGSVVGLVVLLGVVGFFIQKAQQPKIAYPPGFQP
                     PPGTPAPKPAKQKKSKQPQQPAGPPPGFLPNGRPMPAILITSGPRTGERHMLRNGYLI
                     GKQPGCDLIIEDGYTSSQHAQIGMDPNGVCKLYDRGSTNGTYVQGVRITESALQHGTM
                     IRIGSTEMRFLAE"
     gene            28983..30953
                     /locus_tag="VMZ53_16505"
     CDS             28983..30953
                     /locus_tag="VMZ53_16505"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HUS30111.1"
                     /translation="MRKQTGNLIIANQPFNVNAPVINFREPPYWDATREVCQPTATNP
                     NPQCKPGGVPYGNLPHPYTKRYALRGPLRRYGMNPPLEAVKAVIKQFVVHHDGCSSSD
                     MCFSVLQNERGLSCHFLIDNDGTIYQTIDLAFMAYHAAEWNTASIGVEFCNRGDAKKE
                     PNYYSSGKFGPKRNVKPVKINGNTILAFEYTPAQIDAFTALGKALTRLLPNIATEYPQ
                     SSPGQAAWETIPTSASFSFSGYIGHYHLITQKWDPGPFDFKKFCSDLRGSFCFPVFPK
                     PSPDRKPDDKPAVPEDSDALKEAASELYKANEQRADGGYFPVGPWGEARLWHGGVHLS
                     GKTNDPVFAPFPGRMVAARMGPSTPIGSTNFVLLKHDMTLGKSRVQFYSLYMHLADEK
                     NAEKPLEWTQKSESWKAAKGGDVVLLDEPIAAGTLIGHVGTAGPAEWSKAQVHVEFFS
                     NSELFTDIPGSPWQFVDGTAGGRFCDVKTLNDMFDDDKDGTLSRQELSSFYSGGRASG
                     LRYTVVLHVSEWTAEPSWADALKVPKDFKKLKPADIDQLVADQITPGLWWDARVAGHA
                     RLPADGVVFHYHPVTFLAWFNQQLLDAKANAGPEASATDAQEVPKGITDDFGDINGSS
                     MRSSADMSDDPCNQKITLADLVQGFDAPDCTP"
     gene            30950..31126
                     /locus_tag="VMZ53_16510"
     CDS             30950..31126
                     /locus_tag="VMZ53_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HUS30112.1"
                     /translation="MKKQDDETKTTWRPPTRRAFIAMIGAAGAALALREKRRRAGDPP
                     RSPQTGKTLWIGHC"
     gene            31164..32195
                     /locus_tag="VMZ53_16515"
     CDS             31164..32195
                     /locus_tag="VMZ53_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30113.1"
                     /translation="MSLRRLIAVLSVLVIASGSAALAQTPAPATPPAGTGSGSGSGSG
                     SAPADGSAVQPIEDAPPSDMEGRDENPDAPHGTDDGTTAVVATPKAMKPAGYPLEESQ
                     RPITLPANMAEISIGPHFQASPYAGADALRARYGITKQIQFGLTYVMGGVYDDPVNSK
                     KEYKFHVGKAAGVDVTVLLTNFLAVRVGVPVYFDPFAASFAAGAPMKFILNDKLAIGG
                     MEDVLNIRIKRFAPSFYQEAYNAGGAANDMNNTEQSRGRLRFSGFAVYQQNPKTALLG
                     KVGLETDLGAGGGGAAGTSTPSDTVTFIRAGVQYSPKKYLDVGGTIGFDDLSKLGSFG
                     VAGILAVRI"
     gene            32307..36491
                     /locus_tag="VMZ53_16520"
     CDS             32307..36491
                     /locus_tag="VMZ53_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30114.1"
                     /translation="MTSVLMFAAACGDNKHVDRDDAGTDGGSDASIDAPPDALMCTSP
                     QIKCGDACVNPTTDNANCGACGVTCSGTDSCSTDGSSTKCCPAGQHNCGGVCVDWSSD
                     EAHCGSCANACTSGDTCDNGTCCGSNTTVCTTGQGPQCFDLKTDEMHCGTCTTTCTNG
                     QTCTTRTDNTDPKCCTTGQNNCGGTCSDPQTDSANCGACGTNCGAGNSCSAGKCCPNG
                     QTNCNGTCVDLKTDEDHCGSCPTACADNVETCTTRTDGTDPKCCANNYNNCGGTCSNP
                     LTDNLNCGSCGNACGAGQSCSNGICCGPGQTACGNTCVDLNTDNTHCGTCSTNCSTTA
                     NPVCSGGQCASACPAGETNCSGDCVFTLTDHDHCGSCTNACGPSQVCNGGTCTTTCPS
                     GTMACGGSCIDVLTDPNHCGNCGTVCGPTDECVNGSCQLDCPTGQVLCGGVCVNPLND
                     NNHCSTNTSSCGVACGSGNTCELGVCCQNGWKNCNGVCVNPASNANCGEPGSNNCGVN
                     CGSTGEQCKSSGSSHVCCASGEINCNGTCADITSDEANCGACAGGGGQTCGSGQTCSN
                     GECCNGTLVNLNGICCANPATTPGSAACGTPPTCTNTRSDDNNCGACGTVCASNENCV
                     NGSCTTQCLSPNALACNPQICTNTQTDPNHCGAACAQCPTPATCNAGSCQSCTVATGG
                     QFPDVCNDACTNKLTDPNNCGACDTFCGSNEQCSTGLCCSIGTTHCGSGCVDFTAGVP
                     QSDGTTANCGGCGPSFTCAAGSTCTNGQCKCPFGTEPCNGGCVQTATDPDNCGACGKN
                     CTGTDACVSGSCVATCPAPLIKCGDQCVNPDDDDAHCGGCTGNCNFASTGKRCSAGAC
                     VNPTWPPFGVTPTFPAKCAGGGPPIVVDPGAGNICTGNLGQVAFTFGLCSRTAIGPLS
                     QDLYTDAFDSTSGAYTASCLTGGVDDNSKCASLHCVKSLDPCTSTTPGVGPGNGASPC
                     ATGDVCAYPVKCVGGTCIGGGVGVNGTATPVASNTANTHVGGDFWIYGTTGLAMKGNT
                     QVKGILVNQNSIDIGKTSTVYGKSFVGGPWTSGGNTSMTLRSNLFTEVACNQVPGSVT
                     FTNGATCNSVTNWFGNPGYGAGSTNPTEPNPPCGLDNGQDGSLINIKKIVTDYSSSAK
                     NDNFNVTPNIPYSLFDAVSATVRVDLPCGYFYFNQMNIGKDTTIVVHGRTAIFVAGAM
                     RVSQKLILDLDPTATLDIFVGGAVNVSNIVELGSPAFPRRSRLWIGGAGCLGTGTCSS
                     NSDCCSGICTSGVCASGGGGNLSQAMSLSNGGFFNGLIWGGWGTFTHSNPLSMYGSIY
                     TNYYDASGDTNIHYDLGATKIGEECPPPPAGGLCDSCRQCGNQPCVNNHCGQACTSDS
                     QCCAPLKCDLGSGICL"
     gene            complement(36493..37671)
                     /locus_tag="VMZ53_16525"
     CDS             complement(36493..37671)
                     /locus_tag="VMZ53_16525"
                     /inference="COORDINATES: protein motif:HMM:NF039296.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HUS30115.1"
                     /translation="MANAADEEEAAPAAEAPAPSRIRWVVRKLWWLHSGFALSFGFGV
                     MLFARAGLKYADKVMVALLGSWLLMFIAFRFITGPTNRREDEKLIRKGIRVATNYIIK
                     QFYQQMFFFLTPLYASSATWSFASWNWWLAPLLLVCAVLSTMDLVFDNFVMEHRFLAS
                     GMYGLAMFGMLNVMLPLLLGIDHFTGLVIAAAATPASVAFLSFSIRQVLSPQGALLTL
                     GTTGVLLAAVSYGKRFIPPAPLAMSETMVGHGTLGSYECLPPSKHAIRATQLDGLRCG
                     SMLREPGGMKEPVVHVWTYRGHALGTPIQPEVMACDDGDAIVVRSYLPKDRVPADPTG
                     KWSCETYTKGGQLVGLRKFEVLTATGEHAPADTGSASPKTTTPTPDAGAGVTDAKPAD
                     "
     gene            complement(37690..39000)
                     /locus_tag="VMZ53_16530"
     CDS             complement(37690..39000)
                     /locus_tag="VMZ53_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HUS30116.1"
                     /translation="MSQFPRPKTGTGPQGGETPPQFAAFREVPRTGVIYVTTEASRRG
                     YSQTAEDWANLGQGMPEAEALPGAPPRIQDLPIEPGDQEYAPVAGLWELREAIAGYYN
                     DLFRRGMGSKYSAENVAVSGGGRSALTRVAASLGNINLGHFLPDYTAYEELLSVFRLF
                     APIPILLEPERGYAFSGADLRREVLGRGLSAILASNPSNPTGKVVGGGDLSEWVSVGR
                     ELDCALIFDEFYSHYIWRPDLVAQGAIETAARYVQDVDRDPVVILDGLTKNWRYPGWR
                     VTWTVAPKKIIEGVTSAGSFLDGGGSRPLQRKAIDLLHPTAAMAETRAIHTEFGKKRS
                     RLLNGLRDLGFTVDLPPEGTFYVWASAQHLPASISDGMSFFRAALDRKVITVPGEFFD
                     VDPGKRRGGRASRFRRHLRFSFGPAMAKLDLALERFKELIASAS"
     gene            complement(39039..39695)
                     /locus_tag="VMZ53_16535"
     CDS             complement(39039..39695)
                     /locus_tag="VMZ53_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30117.1"
                     /translation="MQRRRQAVRASIASRCATTSSRCATTSSRCLRKSVIALGALVAL
                     VALASCWRNTEPKNPDEQMQTEAQKTVQRDQWERESDAHPPPPNPPVASPPGTYVIAG
                     CNTACNGCSIGKSTSVHGVDVATGKELFPKVVTSGGGGVSCSAYLPLDTHVQLIAKSG
                     DGLVLDKWRAFNADDYCPCAGTDRPICDVTITKEIAAKFTRAYCGADWKAQGAAQIGH
                     "
     gene            complement(39671..40123)
                     /locus_tag="VMZ53_16540"
     CDS             complement(39671..40123)
                     /locus_tag="VMZ53_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30118.1"
                     /translation="MRRRLIAGTVAVAALVAGCGSTSQHSAEPAARQTPATTATSATT
                     ATTATTATTSPPQSTASTATSSTTATTATTTATAATPDPISDDDVVPLDGSPRKGQGP
                     RPPPMHVDPFAPSDKKKVPPPKGKLGNDPIIKSDCRKDPKACNAAGKP"
     gene            40204..41046
                     /locus_tag="VMZ53_16545"
     CDS             40204..41046
                     /locus_tag="VMZ53_16545"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HUS30119.1"
                     /translation="MSIWDGIPQQERVVGLLRGAIERSRVPHAYLFSGPVGAPLLDTA
                     TALAVALNCQVARGEGCGECDSCSKIIGGIHPDVVTLVREGAANIVPIESVRNQVIAR
                     IGLPPHEADVRVFIVEEATAMAPPAANALLKTLEEPPPRTLFILLTTAPEQLLPTIRS
                     RCQRVRFGGGAGLVDADPSRAERISKLGEELAGDRHDPTLPNRVAEGKGDAAPVLVAA
                     ALRLHALAHEAAARADIAAAKRASRRAQTVLSWHTAVAIHNANAQLAIEALIAQLASQ
                     DRVS"
     gene            41043..>41727
                     /locus_tag="VMZ53_16550"
     CDS             41043..>41727
                     /locus_tag="VMZ53_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30120.1"
                     /translation="MSPPPDDEDGPDDGPDDGSDGGDEGADDAIGTGVDSAVAGGGPG
                     GPSSLGSPDPARKRRRRRRRRRDHAGDPADAPRIPDQADAPTREQLAAETPQDGSQPP
                     RDATAAQPRGDSSQAGSAHSQSGSLTGSQPGSQQGRRNDRRDARDNRGGDRRDARDNR
                     GGGGRPGGGHGGGDRPAGGHGAADRPGAGHGGGDRPGAGHGGRDRPGGGHRGGDRPGG
                     GHSGGDRPGA"
BASE COUNT         7365 a        13623 c        13771 g         6968 t
ORIGIN      
        1 ggtcgaggga gctgagtggg gtgcgcaggt cgtgcgagac gatcgcgagg atgtcgtcgc
       61 gaacgccgag ggcggcttgt gcttgggcgt atagccgagt gttgtcgact gcgccgcctg
      121 cgcggtgacc gagctcgacg gcgacctcga gatcgcgttc ggtgtagcgg cggccggagt
      181 cggacgtcat gaggtggatg actccgacgg cgcggccccg cgcgaggagt ggtgcgccta
      241 tccaggactt gaagccgagg gctgtgaagt cgggcgacga gagcatggtg tcggggacgt
      301 cagggatcag gatgggctcg ttggcgcgga ggacttgcca ggagccgttc gcgtctgttg
      361 tttgtggttc gtgtccggcc acgaacttcg cgaggagctc ttgcttggtt ggatcgacgt
      421 gggcgtacgc gacgtggcgg actttgtcgc cctcgagcac gtcgatcacg cagcagtcgg
      481 cgaagtctgg gatgacactt gccgcgagat cgcgaaggag ctcgcgatag tcgagtgagc
      541 gcgcgaggag cttcgtggtt tggtcgagga agcggcgaag ggtgacgtcg gtgttcgtgc
      601 cgaaccagtg gagaatggcg ccggcttcgt cgcggatcgg catcgcgcgg gagaggaacc
      661 agcggtagaa gccgtcgtgg ccgcgcagtg ggaacgtgtc ttcccatggc tcgccgcgat
      721 cgatcgcgcg cttccatcgc gtgacgacgc gttgcaggtg atcgggatgg tgaacctttt
      781 cccagcccca tccctgcatc tcctcgaacg tcgtgcccgt gtagtcgtac cagcgacggt
      841 tgtaccaatg gatggaacct gtcggatcgg ccatccatgc gagctgcgcc atgttgtccg
      901 cgagcgcacg gaattgctcg tcgcgttgcg cgagcttgat ggcagcagcg cgcgcctgat
      961 cgcgaaggac gtgctggatg atcgccgcgg tggctcgcgc gaccatcgcg ctgaagactc
     1021 gttggtccgc ctcggagaac tcgggcgcgt tgatcgagcc gatctttgcg acgccgatga
     1081 cgacgccacc gtcgacgatc ggcacgccgt acagcacgcg aagggacgca cgtgcgagcg
     1141 ccggactgcg gatgtcttcg ggccgagggt tttgcaacgt gagcggctcg ttgcgcgcag
     1201 ccacttggcc cgcgaagccg tcaccgatct ggattacgac gcctacttcg acgtcgcgct
     1261 cgacgccaac gccggcgcgt agatgaagca cgtcgccatc gcggaggtag atcgcggccg
     1321 tatcgatcgc aggcgtcgtc tcgcggagca cttcgagtag cctgccaagt agctcgtcga
     1381 ggctctcgct ctgtaatgcg gcggtcgcga cgcgatcgag gccgagcaat gtgcgatcgc
     1441 gcgtcgcggt gtattgcttc accgactcgg cgatggcttc gtcgatcgcg tcgtcgacaa
     1501 ggcgcagctc gctcgcttgt aggaagccga cactcgggtc cgacagcgcc tgcaagatgg
     1561 cttgcctcag tacgcggaat tccgagatga cttcgctgag gtcgaagcct tcatgcagcc
     1621 gggcaagcgc gtggtgcgca cacgcttcgc gctcggaggc gacccgggac ggatcgaccg
     1681 cgagctgtgc gatgcgatcc aaaagctccg ggatgtgatc gaggagcgtc gggcggtcga
     1741 gcgcgctcgc gcacggtagt gcacgcacag cggcctccca cgaggtcagg atctcgttgc
     1801 gacgtgcgcg caggatgccc gcaacccgtt gtccggattc cggtcccggc cgattggagg
     1861 gaggcggctt tacgaggtcc actggcgatc tacgtacggt gcaggaactg aaccggcgtg
     1921 cggccgacgg gatcacaaga tcgcttaggc acgcgtgcgt ggatctgtgg tctgccgcgg
     1981 agcggattcg cgacgcgcgc gtgttcttga ttctcttcga ccctgcggcg ccagcctgcc
     2041 agacgcacta caagaaccat ccgaacggaa cgctcgtaga agatcgcgac tagtccgcgc
     2101 gaccccgctt cgctccgcgt ttcacttcgc cggaaccaga cggatgtgac cggtgtcggg
     2161 ttcgctgatg agtcgtcatg cgcaggcggt ggaagttgtg gcgatgctgg gcgactccgt
     2221 cgtcggcgtg aagcactgtt gtgatccgaa cgcgaacggc aaggtgcagc gcgcgacgta
     2281 cacgctgttc gcggtcgcag ccgtggcgat gctcgtggct ggcgtcgcgt tctggagcgc
     2341 cgtcgagacc gctgcgtaca accagagccg ctacgagtac gagaccaccg tgctcggcaa
     2401 gcctcggtac tccgtacgga cacggtcact tcctcgcggt tgcgagtcga tcgcatttgg
     2461 cgcgttgctc gcgtccctcg tcgcctgtac cgcggcagtg ctgcgcatgc gcagggagcg
     2521 cgagggttcg accgttcgca tcggcacggc ccccggcgtc gactttccgg ttgctggagc
     2581 gcctgcggag gacttcgcgc tcgtcgtgac gcagagcgat cacttcgcgg tacagcgcgg
     2641 cgatgagctc gtgccggtca tggacgactt cgcggcgcgg ctctgctttg gcggcgcaac
     2701 gtttctggtg tgcgcgatcg agcgcccgcg tcgagtgccg gtgccgctat tcgagctcga
     2761 cgccgagttt tcgcgaatcc tcgtcggcac cgcggcgatg tttgcggtct tcatgctcgt
     2821 cgtgtttcag ctcccgatcg aagcggaatc tgcgtcgatc gagatcagtg agaccgaaga
     2881 gaccttcgtt tcggtggatt ggcccgcgcc cgagagtgcg ccgtctccat acgacggcgt
     2941 cgacggagac tccggcggca gtggcacctc gatggcgctc gaccccggcc gcatgggcaa
     3001 cgtcgagtcg cagcgcgccg acgggcagta caagatcgag ggccggatcg acccgcaact
     3061 cgcgcggcag caagcgatcg agcaggcgcg gtacgccggc atccttggct cgacatcggt
     3121 tacgagcggc aatgcgtttg tgtcgctgac cggatcgttc gacgcttcgc gcggcttcga
     3181 cgacacgaac atctatggcg gcaagctcgg cgacgaggca ggcgagatgg ccggtggctt
     3241 tggctatgcg cgctcgaagt tcggaccggg cggcggcggc acgggctggg gaacgatctg
     3301 cgccggcgac ttcggcacga tcggacatgg cagcggcacc ggcgagggct acggcatcgg
     3361 cggcggtcga tgcggcacga cgtgtcgcgg tcacgtggca gcgacgccgt ccgtatcgat
     3421 cggccagccc gtcgcgatcg gcgctctcga taaggcgatc attcgccgct acatcaagcg
     3481 caacatcgac aagattcgtt actgctacga gaagacgctc ctcgcgaagc cggggctttc
     3541 aggcaccgtg tcgacgcagt tcttcatcgc gccggacggc cacgtcagca gcgcggcggc
     3601 ggagggtgtc gatgtggaag tcgcgagctg cgtcgccgat gtcatcgagg gcatctcgtt
     3661 tccgcgaccc agcgagggtg ccggcgtgca ggtcagctac ccgtttacgt tcgagcgcgc
     3721 gggagagtag tcagcggttt gtgaccaggg cgcacgcgcg tcggcggagt tgcgcgcgat
     3781 ggccgaggca tttgcgagat tgcgccgaga ttcgagcgct cacgcgtggc ggggctgttg
     3841 catccgagct tcggcgggat gaacaagcgg acgctgttgg cggatttgtg tgaccgcacg
     3901 ggagcgacgg agctggcatt ggcggtgcgc aagcgcgcga cgctgccgtg gctcccggtg
     3961 gtgacctttc atcggatcgc gaacgtcagt cgccagtatc ccttcgacga ggaggtcgtc
     4021 gaggcgacgc ccgcgcagtt cgagcggcag ctcgcgatcg tgcgccagca ttttacgccc
     4081 gtgacgaccg ccgacgtcgt gaaggcgctc gacgggcagc cgctgccggc caacgccgtc
     4141 ttcgtgacgt tcgacgacgg ctacctcgac aatcacgcgg tcgccctgcc ccttctccag
     4201 aagtacggcg tcaaggcgac gttcttcatc gcgacggatt acgtgacgcg ccggcgcctg
     4261 ttttggtggg accgcatcgc gttcgccgtg aaggcggccg cgcggcccaa gctcgcgctg
     4321 tcgtatccgc ggccgatggt gctcgaggtc ggtacgcctg ccctgcgcag agcggcaacg
     4381 aagacgctgc tgcgcctcgt caagtcgacc tacgagctcg atctcgagcg attcctcgac
     4441 gagctgaccg ccgcggccga ggtcgcgtgg aacaccgatg tcgagcgaga gctcgcagac
     4501 ggcctgctga tgacatggga tcacgtgcgc gcgctcaagg cggccggcat ggatgtgcag
     4561 tcacatacgt gcacgcatcg cgtgctgcag acactcagct ccgagcatgc gttggccgag
     4621 ctcgtggact cgcgcgcggc actcgagcga gagctcggcg agaggatctt cgcggtctcc
     4681 tatcccgtcg gccacacgat caatgaccgc atcgacgtgc gcaaggccgt cgcgcgcgct
     4741 ggttatcgcg tcggctttac gaatgcgacg ggcgtccagc cgatgtggga cgtcgaccgc
     4801 tacaacttca agcgcatcag cctgccgccg cagctgccgg attcgctgtt ccgcgcgatg
     4861 ctgacgctgc cgatggtctt cgagtaatca ggcgacgctc gacgcggcgt gagcgtcttc
     4921 ggtgcggcga tagatgatct gcacgtgcat gacgtactgg agcagctctt cggtcgcggt
     4981 ccggagctga acgagctcgc cttcgcggag agcgtcttcg acgttgagcg cgagccgctc
     5041 gatcgtcgtg aggccgagct cgtgcgccga gttgcgtagg cggcgcatcg tgagaccgct
     5101 gcggcgttca ccgttgcgga ccgaatcggc gagcgcggag acctgcgcct tcgtcgtgtc
     5161 gaggaacgcg ggaacgcgat cggcgtacca cgggtcgacg tagaccggct cgtagcgctc
     5221 ccagagctgc gccgagacgc gcgtcgggcg aacgagcgga gcgatgccgc caaccgcgtt
     5281 gcggccgtga gcctttgcct gaaggagcgc gccatcagca gccgcgatca gccgctccat
     5341 ctggtcatcg gcgagcacgc gaatcgacgc gaagccgacc gtcaccgtga cgacctcgga
     5401 gcacgtcgac gccgcgtgcg gcatgcgcag accttcgacc gcggcgcgca ggcgttcagc
     5461 gacgatcttc gcgccgaccg cgtcggtgtt cgggagcacc gccatgaatt cttcgccgcc
     5521 gtagcggccg aggaagtcgg atggacgacg caagcaccga accatcgcgt ccgccgcgcg
     5581 ctgcaggcag gcatctccgc cgagatggcc atactgctcg ttgtaggcgt ggaagcagtc
     5641 gaggtcgatc atgatgagag caagcggcgc atgatcgcgg gcggcgcgct tccattcgtt
     5701 ggcgaggagc tccatcgcat ggcggcggtt tgcgatgcca gtgagcgcat cgacgcagac
     5761 caggcgctcg agatcctgct tctccttctg cagcgcgacg atcgcgtcgc acatgtggcg
     5821 ctcgcgactc tcacgcctgt ccgtctccga gcggttccgc atcgactcgc ggatccggcc
     5881 gatcagctcg cgccggcgca gtggccgcgt cacgacctgc gttgctcccg agccaaacgc
     5941 gagctcgatg tccgcctcgt cgcacagcgc gaggacctgg attggctgcg agccacccgc
     6001 gaggcgctgc aggccaccga tgttcccgcg atggatcgag atgatcacga gccggcgctg
     6061 ttgctgcggc tcgccgcgct cgaggagaac gccgccgacc gtcgctgcgg gatcgatgtc
     6121 gaggcaaccc gcttcggcga gcgcctcgcg caggatcggg aggctctcgg actcgacgtc
     6181 gatgagttgc gcgatgaatc cgtgcacggg tgccgagcga gaatccatgc ggcgtgccag
     6241 cgatgtcgcg tcgttgcgcg gtgaggtttc agcgaaatcg cgtcgcaagt gctcgtcact
     6301 gcaaggcagt tgcaggccca acgttcacac gagcggcgca tcgactacag cgactggatg
     6361 tcccagcgag cggcgccatg tgccgtagcg tcgcgtcgca attctgcgcg cgtgcgcacg
     6421 ggtgtgccgg ttcgacgcgt ccactccttg ggtaacgagc cattgcgcgc gcagaacgcg
     6481 tgcgatttcg acaccgtgac ggccactcac gatcgctggg cgcacggcct atccgttgca
     6541 gtaatggccc caacgcgaat gagtgatgga gttcctcgtc gcagactgct ccagagcgtg
     6601 gatagcatcc cgctccacgt gccgtcggcg cgaatcggta ccgatcccgg cgaggagtat
     6661 cggtgcatgc tcatcatcga gagcgatgac gtcctgcgcc gcacgaccac gcgccagttc
     6721 caggccctga agtggaacgt catcagcgcg cacgacctca agagcggcct cgagctcgcg
     6781 atccggtatc agccgtcggt gatcatcagc gatctggcgc tgcccgacgc cgccggctac
     6841 ggctttgcgc gaaccctgcg aacgctcatc gatcacgaca ttcgcgtcgt cgggatgacg
     6901 accgatccgg agaatctgtg cgctgaagtt cgcgctgctg gattcgattt ggtgttcgca
     6961 aagccgctcg acgtggctca gacccacgag cagctaggac ggaaggcgaa atgagcgacg
     7021 acaacgagcc ggagcaacac cccgcacccg ctccggcgac gacgccaacg ttcgtccact
     7081 cgaagccgct gctcgatccg gtcctgcgcg aggaagcgga gttcgcgaat cgcgactacg
     7141 cggcgcacgc aaatgatctc gagctgagcg acgaaatgtt cgcgaagtat tcgcgccccg
     7201 ccgagctgtg ggactggcgc cagctcgccg cgatgatgct cggcaacgtg aagggcaagg
     7261 cgctgctcga ctacggctgc gggatgggcg aggagtccgt ctactgggcg aagctcgggg
     7321 cacaggtgac ggcgatcgac atctcggaag tcggcgtctc gacgacgaag cgccgcgccg
     7381 agtaccacaa gctcgacatt cacgcgttcg agatgcgctg cgacccgacg agctttcccg
     7441 ccgcgagctt cgatcgcatc cacgggatgg gcattctcca tcacgtcggc atcgacaatg
     7501 cgctcgcaga ggtctggcgc ctgttgcggc cgggcggcat cgcagtgttt ctcgagccaa
     7561 tgggcgatca ccccggcatc gaagccgtga aagagttctt gatgcggcgc gcccgcttcc
     7621 tcggcgactt cgacgaggtc accgatcacg agcacaacct aaggtgggcc gagatcgagg
     7681 aagcgacgaa gcggttcgct cgctactcgg ccgtgccctt ccacctgctc taccgcctca
     7741 agcggttcgc accgtacaag ctgcacacgg cgatgcgccg gatcgatgca gcgatgctga
     7801 ccctcgcgcc gagtctcaaa cgctattgtg gcgcggtcgc gattcaggtc tagaaataga
     7861 ccgaaacgca aaacgagcca cctttgcagg gcggctcgct gcgtcgtcgc ttactgcttg
     7921 atgacttcgc cggtcttgag gtcgagcgcg tagctctgac cgccgtcgaa cgtgagcgtc
     7981 gtcggaacgc cactcgcgcc gtacgtcagg tgagcctgcg aatcgaacac gcgacggtcg
     8041 ccgttgcgcg tgcgctgcgt gttgaccttg aagtcgatgg tgccgaacgt cgggatcgtc
     8101 gtgtcgggca ggaagcgaac ctgctcgtag agcgcttcga acatgacgac gtagtccgcc
     8161 ggctcgctgt tcgtcacgac ctgcgacttg aggaccgtgc cgtcggggcc gcggaagcgc
     8221 gccttgccga ggttgacgtc gcggatctcc cagttcacgg cgcgctggaa gtcgttcatc
     8281 gagatcgtgc ccatcgtctg cgtgccggag atgttcagct tgaggtggcc gtggtgcgcg
     8341 ttcgcatcgc aaggcacgcg gatgtgagct gccgagccgt cgttgcagtg gaggccgaag
     8401 ttgatcgtca gaccgttgag cgtgccggtg ccgagtgcgt cgacgccgtc gtcgccggtg
     8461 atcgcgaagc cttcgggaag gaccgcttcg ttacgaacga tcgccgcacc ggtcagctgc
     8521 acgctgaact ccgcgcggag atgcgccgag aggatcgccg cgagctcgtc gtagtcgcgc
     8581 gcggtctctt gggggttcgg cgggtcttcg ggatccgtcg ggtcaaccgg atcgaccggg
     8641 ttgtccggaa gctcgtcttc ggggcgggcg ttgtcgttac atgcggccag cgcgagtgcg
     8701 aagccagtca gaagaatttt gcgcatgtcg tcctccgccg gagggaatca gcaatcggcg
     8761 gaccagaaca tgtgttgcga aatcattgga gcgagcgatc gcgttcgcgc gcttcgcggt
     8821 gggcgtctcg cgatcttgga atacgttcgc gaccacggcg ctcggcgtgg cattgatggc
     8881 gctgtgcgca cgcgcggcag gcggcaaaaa ggcaacaggc gcccatacgg cggttcatag
     8941 cccggtttcg tgatctccct ctcggctttg cggtaagacc ggggccatga cgattcgagc
     9001 gcttggatgg ttcctcgtcg gggtgtgcgc gctggcagcc tgcggaaaag acagcaaggg
     9061 tggtccgagc gtggccgacc tcgagaaacg ctgcgacgcg ctatcgaagg cgtgcgccga
     9121 tacggacaag cacgtcgaaa agctcggcgg agagtgcaaa gccgcagcga agacagagag
     9181 cgagaggggc tgcggcgata aggcgattgc cctctacaac tgctacgaga acgcggtctg
     9241 cggcactggc gatcgcgtct gggccctgcc cgacctgaaa gtgctcgccg agcgccacaa
     9301 gaagtgcgac gccgagcagg ccgcgctcac cgcatgcgtg gacaagaaat agtcatgatg
     9361 aaaatcggat ttgcggggct ggttgtcgtc gcgctgtgcg ccgtcgcaaa ggcggatgca
     9421 ttcgagtgca agcccaccga aacgcaggcg gagtgccaca cgcgcctcaa gtgcaaggcg
     9481 aacgaagagg tcgaggattg ccagaagcgc ctgcgcgcgg ctgcattcag cggcggtcag
     9541 agcagcggcg gcgataaccg cggcggcgat gatcgcggtg gcgaccgagg caatgatcgc
     9601 ggtggcgacg atcgcgggaa cgaccgcggc aatgatcgcg gcaacgaccg cggcggtgat
     9661 gatcgcggca acgaccgcgg cggtgacgat cgtggcaacg accgcggtgg cgatgatcgg
     9721 cgaggcagcc gacgtagcag ccgtcgtcgt ggcggtggcg gcggtggtgg cggtggtggc
     9781 ggtggcggcc acggcttcga ggcgaacaag accttcggcc tcggcctcga gctcggcgag
     9841 ccggctggcc tcaacggcaa gtacttcttc ggtccgtcga cggcgctcga cttcggcgtc
     9901 ggctggatct atcgccacta ttactacggc gacggtctgc acctgtacgg cgacatcttg
     9961 ttccacccgg tatcgctcgc ctcgacgcct gcgctcgaga ttccgttgta cgtcggtccg
    10021 ggccttcgct actgggactt ccagtactgc gtcggtggca tctgcgatta cggcggttca
    10081 gcaatcggta ttcgcgtgcc gatcggcatc tcgttcgact tcaacaacgt gccgctcgac
    10141 atctttgtcc agttcgttcc gacgatcgac ttcatccgcg gcgactacta cgaccggtat
    10201 cgggaccgga atcacttcgg cgtcgacttc tcggtcggcc tgcgcttctg gttcaagtag
    10261 tcaccagtcg aggccgcgaa ggttcgtcga agcgagcacg tggcgcggcg aaccgccgcc
    10321 cgccgcaacg atgtagatgc cttgttcgcc gatgaaggcg atctcggttc catccggaga
    10381 ccagcgaggg ctccacccct gggctgcgac gagcggcgtc tcgtcgtcga cggaggccgt
    10441 gctcgtgaag atctttccgg gcgcatcgta gttgccgtac tgcgacgaga tgacgaacac
    10501 ccacttggcg ccgttcggtg accaatcgag gtgcttgttc gggctgccga cctcgatgcc
    10561 cggctggacc gcggccctga agtcgaactc gtgatagccg gtgccgtcgg gcttgatgac
    10621 accgaccacc tcgccgcctg cgcaatcagg gcagtcgtac gccatcgaca gcgaaatgac
    10681 ggcgcccgac caaccgagcg agttgacggt tccattgatc gccgacgata cgaggtgatc
    10741 gcctgtgcca tcggcgttca cgacatgcag cgtcgttccg tacgactcgt cgatcatgtc
    10801 ctcgccgtcg ttgacgatga acggctcgcg cggatacgga tacgcgagct gcgtgccgtc
    10861 cggtgaccat gcggcgggcc ccagcgtcgt cgaatagacg aagtcgttga tcttgcgctg
    10921 acgcccgacg gtcatcgcga cgacctgctc gttgcggcca ttcgcgtcgg cgacgaatag
    10981 cttgaagtcg cgaacgaacg cgatcttcgt gcggtcaggt gagatcgacg gatagagctc
    11041 gccgcctgca gtttgcgtga ggcgcgtcgt tgtcgagcca tcgaggacat agaggtccgt
    11101 cgccggttgg ccatcgagcg cggctgccag gacgatcgag ctcgtgctca gcttcgccgt
    11161 cgccgcgtca ggggcgctcg ctgcatcgtc cgcgcgcgcg tccggcgtcg tcgttgtgcc
    11221 gccgtccgga cccgttcctc ctccgccgcc tcctcctcca ccgggatgac tggagttaca
    11281 cgccgccgcg aaaatcaccg agctgacgat gccgaatacg cgcatgccgt aatgcaatcg
    11341 gcggtgacat gccaacgcga tttgttcgga ctagaagccg gcgaccgtga tgttcgtctt
    11401 caccgactgc gcgatgaagc actggtcgtg cgcatcgtga tgcagtcgtg cttcctcttc
    11461 gcgcgacggc tgctttgtcc cgccgtacgt gatcgatggg tgcaacgtcg tctcgctcac
    11521 ccacgcgacg ccgcgctcgt tcttcgccat gcgaccgacg gcgcggtcct cgtacgagtc
    11581 gacgacgaat cctgctttga acgcgagcca cacgaacgtc aggaagtggc agctcgagat
    11641 cgctgcgacg aacgcctcct cgggatccac cgcggcagga tttgcgaacg cagcaggaac
    11701 gtttccgggc gccggcgaac caggcacggt gaggccaccg tcgaatgtcc acgtgtgctc
    11761 acgcgagtag cggcccttga ggaagtcctc gggtgtgcct gcgcgctgcc aacgaatgat
    11821 cgcgatgtgc tcggccatat cagtgagtcg tcgggatgta agagaggtac ttgagcacgg
    11881 accgcctgcc ccacgtcgcg agcgcgtagt cgacgagatg cttcggtaca aacgaatcgt
    11941 tgttaccgac gagccgcatc aatgcgagag cgaggtcggc atcggcgatg caccactcgc
    12001 tcgcgatcga ctgcttaccc ggcgcaacga gctggctcgc gacgcgcacg agctcatcgg
    12061 cgtccgccct gcccttctcg gagagcgggt tcgtcaccgg cttcatgaag acggacgtcg
    12121 tcgggcgatc gttgcgaagg cccatgatgc tcgtgcgtag ccaagagatc acctgacgtg
    12181 cacgcgcacg ctcgcgagca tcggccggca agatccgcgg atagctcggc ggcgcgaact
    12241 gctcggcgag gtactcgctg atcgcgctcg actccgtgag ccagaagtcg ccgtgcacga
    12301 gcgccggcac gaacggcagc agcgccttcg tcttgagctc gcccttgagg tcgtcggcaa
    12361 tcggccgtcg aatgggctcg agcgtgtact tcaccttcag ctcgtcgaga gccaccatcg
    12421 cgtggaagac ccacgggctt tcccacgccg cgtccccata cagcgttagc tcactcatgg
    12481 tggacatctt acctacatcg cgacgtcgat tgggttgtgc ctacgatggg actgccgtgg
    12541 aggacaggtt cggcccctac tccgtcttcg agtgtctcgg agccggcggc atggcgacag
    12601 tccatcgcgc atcgatcgag ctcgaggacg acgagacccg agaggtcgcg ctcaagcgcc
    12661 tcttgccgca gctcgccgcc gacaagcggt tcatcgacga cttcattcgc gagggcaagc
    12721 tcgccgcgca gctgaagcac ccgaacatcg tccgcatcct cgagctcggc cggatcggca
    12781 agacgtactt cattgcgatg gatctcgtgc gcggtcgccc gctgatgtcg ctcatgcgca
    12841 aggcgtatca gaagcgcctg cctgcaccga tcggtgtcgt gctcggcctc atgcgcgagc
    12901 tgtgcgatgc actcgagcac gcgcacgacg cgcagatcgt gcaccgcgat ctgacgccgt
    12961 cgaatctcat cgtcaccgac gacggccacc tcaagatcat cgacttcggt gttgccaagg
    13021 cgctcgtcgg aaatctgcag acgagctcgg gtctcgcgaa gggcaagctc ggctacatgt
    13081 cgctcgaggc catcggcggc aagcagctcg atacgcgcgc ggatctattt tcgaccggcg
    13141 tcgtgatgtg ggagctgctc gcgacgaagc ggctgttcaa gggcacgaac gagatcgaga
    13201 tcatccagca gatccgcgat ggcgatgtgc agccgccctc ggtctacaac aaagattgcc
    13261 cgcgcgacct cgatgacatc gtcctgaagg ctctacagcg cgacaaagag gagcgctggt
    13321 cttccgccgg cgagatgcgt gacgcgctcg acgagatccg gcgctttcat cgcggtgaca
    13381 cgtccgcaaa cgccgtcgtc aactggatga ccgagctgcg cggcgagacc gtgcgcggcc
    13441 cgcggctcga gtccgatggt gagagcacgc aggtcgggct cgagaccgaa gacatgttcg
    13501 aaggcgacgg aacgagcggc ccgcgcgagg tcggcagcga tgcgctcgca ttcggctcgc
    13561 agcgcggggc tcagcccgta tacgcccgcg gctccgacgt ccaaacgtac gacgacacga
    13621 tggacgagcc cgagagcacc gaggtcgccg acacgaccgg gcctaagttc aaggaagtcg
    13681 acacgatcgt cagtacgttg catccccgca agtcctgaga cgacgcgccc atgcggtgca
    13741 tcgaagtctt atgtacgcgg atgccatcaa ggttcgtgaa gcacgcgagc agtacttcat
    13801 cgccaacggc tttagcgacg cgacgtaccg ggaggactgg gcgaggatca agctcggccc
    13861 ggtccccgtc gtgtttccga acacgaagtc gcgcaaggag gcgctgccga tccacgacct
    13921 gcatcacatc gcgacgggct actcgacgac gatcgaagga gaaggcgaga tcgccgcgtt
    13981 cgagatcggc ggtggctgcg ggcgctatgc agcggcgtgg ctgatcaatg cgggcgggtt
    14041 cgcgctcggt ctcgtgacga accctcgcaa gatctttcgc gcgttcgtgc gcggccggca
    14101 tagccgcaat ctctatcgcg aaggttttcg cgactcgctg ctcgacctgt cggtcgcaga
    14161 gctgcgcaag cgcgtcggca cggacaccgc aacgccggtc gcgaccggac aagacaagct
    14221 agcgttcgtc gcgtggtgct tcgccgtgag ctggccggtg tcgctcgcga tgctcgccgt
    14281 gggcgtcgtc ttggcgcgct gaccaactcg agaagacacg cgccgcgcgg tgcggttgca
    14341 cgaaaagaag cgcccaagct cgcgtagatc caacgcgtct ttcatccgtt tcgacattcc
    14401 ccaggaggac gacggatgaa gcccatgatt cgaacggtgg ttgctctcgt aggtgcgacc
    14461 ggtgcagcca ccggagcgct gatgctgcat cgctctaacg cggccacgcg gcccactgcg
    14521 ccgcagctcg acggaccggt cgcgtgcggc acgcctgcac cggcacacgc cgatgccaag
    14581 ctcacccacg gcaagctcgg cggtgcgttg tccgctggca agctcctgcg caatggcgac
    14641 ggcgaggcgt tcgccgcgtt cgagctcacg acggacaacg tcgcgggcca acgccctcct
    14701 ctcgatctcg cgatcgtgat cgatcactcc ggctcgatgt cggatggccg gctcgagcac
    14761 gcgaagtccg ccgcggtcgg catcgtcggc aagctcggcg cgcaggatca cgtcgcgctc
    14821 atccagtacg acgacaccgc gcaggtcgtc tttcaatccg cggcgatgga tgcgcgcggc
    14881 aaagagtcgc tgatgtccgc gatccgtggc ctcgatctcg gcggcggcac gaacctgcac
    14941 gccggcatgt tactcggtcg ctcggaagtc gagcgcgcgc atgcggacgg tcaggtcaac
    15001 cgcgtgatcc tcctctccga cggtcgcgcg aactcgggcg tcgtcgacac gccgcacatc
    15061 gcggacgcag cacgcgacgc tgcgaaccat ggcgtgcgca tcacgtcggt cggcatcggc
    15121 ctcgatttca acgaggacct catggaggcg atcgccgaag caggccgcgg caactatcac
    15181 tacgtgaaag aagcctcgga tctcgatcgc gtgatcgccg aggagatggc cggcattcag
    15241 acgaccgtcg cgacgaacgt cgagctgcgg ctcacgcctg catgcgacgg cgtgcaaatc
    15301 gccgaggttc atggctacga atccaagcgc gagggcacgg cgttcgtgat cccgatggcg
    15361 gacctcgcgg cgagtgacag ccgcaagctg ctcgtctcgc tcaaagtccc ggcgaaccag
    15421 aacggccaag tcgcgacgct ccacgccgag ctcgtctatc gcgacgcgaa gggaaccgag
    15481 gtgcacaagg ccaagctgga tctaggcctc gaggtctctg aagacgcgaa cgcggtcacc
    15541 gcctccatcg actcgaacgt catggcgcag gcactgcagc tccaggcggc gaagtcgatg
    15601 cgcgaagccg cgcaggccta cgagcagggc gaccaagccg ccgcggtgcg catcatcgat
    15661 cagagcacgc gcgacatctc cgagaagagt gcgcactaca agatcgaggc gaagaccgca
    15721 gggacgcgcg ccagcctcgg tgagatgcag aagaaggccg ccgagtacaa gcccgacagc
    15781 tacgagggca aagacatgct gaaggccagc aagtccaaag cccgcgtcat gtcgaagaag
    15841 tagcttcgag cttgtcgagc acggcgacgc cgagcggagt tacttcctcg acgataaagg
    15901 cccgccggcc gtcgccttcg gcgaagcgtc ccgagatgca gccacgctcg tagagctctt
    15961 gcgtctcgct cgacgcgaac acctcgagca ggtgcttgca gtagatgcgc ctcagctctt
    16021 gcaacgccga tacagacagg ctcggaactg gacccatgcg ggcactatcg ccgatccccc
    16081 tgacaacggg cccgctgaac cggacttctt agcgccagcc gacttcgaga ccgacgcacg
    16141 accgggcgga gatgggatcc ggctcgtacg ttccgtagcc gtcgaggttg acgtcgtcgc
    16201 ggttgacggg aatcgcgagc tcgatcgagc cgacgaggcg cgcggtgtcc gtcatgcccc
    16261 acgcgacacc gaagccgccg ccgactgctt tccagcggcg atccattggc gtcgatccgg
    16321 tcatcatgcg cggcacgacg ccggccatct gccgcgcgct cgcgagctcg ccggcctccg
    16381 aaagcaccca gccacgcagc ggcgtcttca tcgaggacca accgacgcga accgtccaca
    16441 cgttgaggcc gagctcgacg tgatcgggag actccgcgac tacgtacgtg tgctccggct
    16501 tccaccgccc gaccgcgagc tcggcaaaga tctcttgatg gcgaacctgc gcagcgagct
    16561 cgccgccgta gttcacgccg ggaagtgcgc cgatgccgga gagccccgtc gcgcgaacgc
    16621 cgaagcccca cacgtgcgtc gtcgcgagtg gatcgcggac cggcttgcgg acgtacacgt
    16681 tcggcgcgac ctcgggctcg cgctcgacga catccgtctc gccgggcgcg cgcgtgttgc
    16741 tcgcgggcag cgggctcgac atgtcgacgg actcgtcgat cggtggcgtc gcgccaggct
    16801 gcgcaactgc gatcgacgac gcgaacaagc tggtgaatac gacgagctgc gaaagcttcg
    16861 acatgcgctg tctcccttcg gacatcctac gcctctgccg tcgaagatcg tcgaggtttt
    16921 cgcccggcag gatgcggcac acgcgtgggt cctggtcgga caccgtcgcg tcgcacaagt
    16981 tgcgccactc cgtggccgga tcgcgcatcc ggagagtaga tcccatgccc gtttgtgcgc
    17041 ggccctcaga tttccgtcac cggccgaggc gacgccggcc gcgcgcgcca ccacggtcgc
    17101 gatgccgcgg cgccaggtca ctcgacgtca cgagctcgta ctcggctgat tcgacgtccc
    17161 caatgacgag cgcagagagc gtcgcgacaa acgccttcgt gccgtctgcg tcggcgtgcc
    17221 gctgatccgc ggccacatcg ttcgtgcggg tcatcgcgat gtagcgattg ggcgcgctcg
    17281 tgtcgcgata cgtggtccac atcgaatcat cgaggtgctc gcgcacgtac atcgcaaacg
    17341 cgtgcatcgc ccgctccgct tcgtcgcgag cttcgggccg gacctcgaag cgctcgagct
    17401 tgtagaccgc catgcggtcg aagctactac ggcgcgatcg cggtcgtcgt catcttgtcg
    17461 gcacgcgctt cgacaacgtg cgagtgaaac gtcatgcccg ccttcgtcgc gctgatcgtg
    17521 atcggcccgc tcggcacgtt gaacatgaac gctacgccgt cggtgttagt cgacatcgcg
    17581 ctaccgctcg gatagccgtt cgagcccatg tagcgatacg cgccggccgc cggcatcgcg
    17641 gagatcgtag cgcccgcgac aggcattccg ttcgcgtcga tgatcgcgag cccgatgagg
    17701 cccttgcccg acatctgatt gccgctcgcg aggttgctca agaagtcgcg attgctcggc
    17761 gtgagcatgt tgaggtcccc gtcggtgtcc ttggagaacg gtgcggtcgg atagagatag
    17821 aggtcgacat agccggactt cttcgcgagt acgaagccat cgatcgcggt cccgttcgtc
    17881 atcacggtca tcgtgtactt gccctgcgcg tcgctcgtcg ccatcgcgat tggcgtttgt
    17941 tgagccgcgt tggagtagag cgcgatgctg acgccgctca ccgggctctc gccggagatg
    18001 ccttgctcgg tgacgtcgcc gctgatcgtg aacatgctgg gcgccaccac gggcgcgtcc
    18061 atgaagactt tcgcgtctgg cgtgtcgttc gtgtcatcgc cgttgttgcc accgttgttt
    18121 ccgccaccgc acgcagcgag cagacacacc acgagagaga gagatttcat gccgcgcatg
    18181 ctgcacgaaa ccgtgtgctc accgacggat cagtcactaa ctgttcgcgc gcacacgacg
    18241 cgcaccgagc tgcgcattgc gtgtaggttt cgagcatggt cgacgatccg gcagttcgtt
    18301 ccgtgcgcgt tgcagcggcg ttgtgcgatc gattcgcaga gctgctgaag ggaccgacga
    18361 tcgtcacggc gcacaaaggc cgcggcgcat tgatcggcat caagggcaac gaggacgact
    18421 tgtcgctgcg ccttggcgaa gcgcagcaga tctatcccga gatctggagc cacctcgacg
    18481 aagcgcgcaa agcattcggt gcgcgcggcg ttgccctgcc cgcgttcgat gcgctgcgcg
    18541 agacggagcg gcagttcggc ggcacgggcg caggcgtcga catgaagttc acgcagtacg
    18601 gcgatccgac gtacggcatg caggagaccg tcaagaaggc gaacttcaac caagcgggac
    18661 tcgcccgcgc gcgcagtgcg atccaagcga tcacgctcgc gacgccggac atcgactggg
    18721 ccagcatcga gaaggcggag gcgaacgatc cgcatgcgaa ggcattccgc cgcgcgacga
    18781 tcatcaagcg catctggatg ttcggtttgc tcatcgcagt gatcggcatc ccgttctggt
    18841 ggctgttgtg gtctcgccac aaggagaacg agaagcgcga gaagtggcgc aggcagtacg
    18901 agcagaccag ctaccagccg cccgcgccgg tgttgccgcc tacgccgccg gccgacctgc
    18961 tgcccgccgc ggagcaagcc gagctcgcga atcaggcggc tgcactcgac ttcaagctcg
    19021 ccgagtcgct cacgaactgg gagcagctca ccgcgcccgc ggtgctcgcg aagctgaagg
    19081 ccggctccga gccgtgcgcg tatcccgtga cgccgccgcc gaaagacgcg ggcgatgact
    19141 tcatcaagac cggcgacaac gacacgaagg tcttcgcgcc gagcgacttc atgggctacc
    19201 tcgcgaagga cggcgtcgtg ccgtccgatg agctgtacga gtaccagcgc aatttgaagc
    19261 aagtgctcgc gctgttcgag aagggcgccg cgacggctcg caccaaagag cgatgggccg
    19321 tgatcacgga gccgttcatg ttcgtgctca tcgatcgcag cgacgagccg gagatcctcg
    19381 acatgacgag cacgccgatc tcgttcaagc cggggcgcgt gacggggcgc gcgtacgtgt
    19441 tctcgatcaa ggacggcaag atggtctgca ccggcgcgat cgacgcgaag agctcgccgc
    19501 cagctgcagc ggctgcgtat ctcgacggcg tgacgagcga gtcggtggcc ggcaaggtcc
    19561 tgcatcgcga gctcgaagtg cgcatccgcg aggggctggc gaacctaaag gccgtggcgc
    19621 cctgagcact tgcgaattag gccattgaca cagttcgaca actgtcgtac tgacaacgcg
    19681 tggccaagga cgacgtagac aacctcgcgc ggatgttcaa ggcgctcggt aatcccaacc
    19741 ggctacgcct gttcctgaac ctcctcgagg agagcaagct cgacctcgcc aagggccgca
    19801 tccacgactg cttcctcgtc aagctgctcg gtgggctcgg cgatatcggc gcaccgacgg
    19861 tatcgcatca cgtgaaagag ctctccgacg caggtctcat ccagacgcag cgcgagggca
    19921 agcagctgat ctgctcggtc gaccccgaag gtctcgagca gctacgtcag gcactcgcga
    19981 acggctgagc cgctcgtcat tcccagtttc cagccggcgc gccgctcggg gcgtgaaggc
    20041 cgacctctgt ccactccaca cttcgaaagt ttagaaacca tgacctacca acagatcgcg
    20101 atcgagaagt tcggctccgc ctccacactc catgtctcgc agctcgcgcc ccgcgcgccg
    20161 ggcgcggacg aagtctcgat cgacgtcgcg tactcgggcg tgaacttcgc ggacgtccag
    20221 atgcgcctcg gcttctatcc agacgcaccg aagaagccgt tcgttcccgg ttacgaagtg
    20281 agcggcaccg tctccgcggt cggcgcgaac gtgaaggaca tcaaggtcgg cgacgccgtc
    20341 gtcgcgggca cgtacttcgg cggctatgcg tcgcacgtga cgattccggc gcgtcaggtc
    20401 ttcaagcttc cgaagacggt cgatctcgaa gcgggcgctg ccctgcccgt caactacttc
    20461 accgcgcagc tcgcgctgtt cgagatggcg cgcgtgcgcg ctggcgaccg cgtgctgatc
    20521 gagtgcgcga ccggcggtgt cggtgtgctc gcgatccaga tggcgcgtca tgcaggcgcc
    20581 gaagtcacgg gcctcacgac gagctcgcac aagaagggct tcatcgagaa tctcggcgcg
    20641 aaggcctaca cgcgcgaaga gttccgcgcg aacgaaacac tgcgtggcta cgacttcatc
    20701 ctcaacgcat cgggcggcgc cgagatcaac tggcagcgcg cgcgcctcgg catgacggga
    20761 cgcatggtgt gcatcggcct gtcatcgggc gtcaaagatg gcaagcgcaa cttcgttcgc
    20821 atcgcgaccg ctgcgctccg cactccgcgc atcagcgtgc tgaagctctt cgacgccaac
    20881 accggcatct atgcgctcaa cgcgctgcac gtcctgcaag acgagacgtg ggtcgagaag
    20941 ctgacgaagt cgatggtcgg cgtcgagcag atgaacttga agccgcacgt cggcaaggtg
    21001 ttccccgccg ctgaagtcgg cgcggcgcac gcattcctcg agacgaagca ggccaccggc
    21061 aaggtgctgc tgcactggaa cagcgcgaac taagtactac tgcagaacgg gcgcgagagt
    21121 cgtcacgcca gcgccccacc agctgatgcc cggcgtctgc gtgaccatca tcgcgccgcc
    21181 gttgctcgga tcgatcagca cgaactggcc gccgttggtg aagccgtaga ctttgccctt
    21241 gaagaacgcg acgccccaga tctgcgaata tccggtccca ccgccgatcg cagacgcggc
    21301 aaacgtgttg ggcgcgaggc gcgcgagctt atcggcgccg ctgaacggac cgttgacgac
    21361 cgtctgcacg gtgccgaatc cctcgacggc gacgagatcg ccagacgatt ggaagccgcc
    21421 catgtcgccg acctgcgtgg cgctgccggt catcggatcg atgcgcatga ccttgccgtc
    21481 gctgttgttc gttccaacga ggatgtcgtc gccggtttga ccgagcatcg acgcgggcac
    21541 gaacgagagg ccgttgaacg tgccgttgag cgaattcgag aggagcgtcg tcgcggcggt
    21601 ggccggatcg atcttgtaga cgcggttgta cgagatgccg atcatcacgc cggctttgtc
    21661 gatcgcgatg tcggtcatcg agtcgctgcc gacggaaccc cagccaaagg gcgcgaccat
    21721 ctgcacggcg agtgtgtctg gatcgacgcg atagagcgtc gaggcgctgt gcgcatagac
    21781 gtagacgatc gtgccgtcgc tgccgctgcc gctaccgcta ccggttcctc cgccaccgcc
    21841 acctggatcg ccggtgtcgt cgccgcccat gtcgtcgcga tcgttcggac cgcacgcgac
    21901 caacccgaaa acaaaaaccg aaaccagtag cccggctttc ctcatcggcg cggacgatag
    21961 atccagctcc cgtcgcggtg gaagcacaga atgcgcgcgc aggtgatcga accgcgcacg
    22021 agcggtcgaa ccaatcgcgt gacgcgccgc gccttagtgc tcggcgaccg gcgactcgag
    22081 acgacgatac gcggccgtca cggtgcggcc cttcgcgctg acgttgttct ctttgaagct
    22141 gtagcgctcg cccgcgatga tcacgccgct cgggcgatcg accatctcct gcgtcatctt
    22201 cgcgacctga tccggctcga agccgcccgc atcccagtcg agaccggcaa tgttcgccca
    22261 gcgcgacgtg ttgagcgcct tctcggtgac ggtcgggcac ggcgcctgga tgcagcgaac
    22321 gccgttgtcg tgcacgcgtg cgaacacgcc ttccgacacg ccctcgcctt cggcaaccca
    22381 cgcctcggtc acgacgaacc ggcccatgtt gccgtacgac gcgtacgtct tcggcgcgaa
    22441 ccgtccacgc acgagcgcaa cggcgccggg cgtcgtcgca tcttcttgcg ccttcgcgag
    22501 cagcgcgccc tgcagctcct cggacagctt cgcctcgctc cagtcgagct cgggcgtgta
    22561 gcacgaccac ttgctcgtcc cgttgtgaca gacggtggtc gtgcgattca cgcgctgcag
    22621 aaagaagccg ccacagagcg gcgagacgca cttgcgcatg tcccgccaaa tctcgaagta
    22681 cgtatacgag ccgtcgaccg cgccatcggc cttgccgtcg agcgccgcgt cttcctgcgt
    22741 ctcgcccgca agctcgtcat cgacgcccgt cgacgcgcaa ccagcgatcc cacccgaaat
    22801 aatcgaaatg gccacaaagg ccttaagcga acgatgcatt agccaaactc ccgaagctaa
    22861 gagcgaacag aattgatgcg gcgcggctaa gcaagcaccg cgccgacgac gaccctcgat
    22921 cggcgcgtag tgatccgcgc gcaatcacgg cgtttcgccg cgacacgacg cgcgtcgcag
    22981 ggtggctcct cgagtcgccg ctcgcgccac gatggcgcag caaaacgtgg cactcgctac
    23041 cggaaattca gtccgaccga atttcgacct gctcgcccat gctgtccgcc gtggtccccg
    23101 tcgaatcgcc gcggatgatc gacgcgatcg gcatcgtcgc agtgagctga aggccgatca
    23161 cgccgcgcgc atcgacgccg atgacgcccg cgctgaggcc ggtgatgcgc gcgagctcga
    23221 gcagcgactc gatcgtcgca tcgcgcagcg acgcgcccgt cagctcgcag cgcagcgcga
    23281 tcgatcgcgt gagcgcgacg cgcaagatcg cttcaccgtc gcccgtcgcg gacagcgcgc
    23341 agcgcgtgtc ggcccacgtg cccgcgccgg gaatcggcga gtcgccgacg cgtcccgtgc
    23401 gcttgccgac gatgccgccc gtcgacgtcg ccgctgcgaa gcgcccgcgc tgatcgcgcg
    23461 cgaccgcgcc gaccgtaccg ccgctcgtct ccttcgcacg cccttcgagg aacgctgcga
    23521 ggcgctcgcg agcgcgctcg gtgacgagcg tgcctgtcgg cgccggccgg atgtgcatgc
    23581 tcgaggcgaa cttccaggcg gctgacccgg cgaggatcac atgctcgttc gcgtcgagca
    23641 cggcgcgcgc gagcgcgatc gcgttgccga gatccggcac cgccgcgacc gcaccgacgc
    23701 gctgcgtggc gccatccatg atcgatgcgt ccgtctcgac gcggccctcg cgcgtgagcg
    23761 ccgagccggt gcccgcgttg tactcggggt cgtcctcgag cacgcgcacc gccgcgacaa
    23821 cggcatcgag cgcgctgccg cccgcgacga ggatcgcatc gccgacttcc gccgcgcgcc
    23881 ggcagccctc gcgcagctgc gcgtgtcgat cgtccgggac gacacccgca ccaccgtgca
    23941 cgatgatcgc cgagctgctc atttacgttt cccgccgatc atcgcgcgcg actcactcgg
    24001 acgccgcgca gcggaatccg acgatgacgt gtcgcgcgtc gacggggcga ccatggcgcg
    24061 aagcgccgcg accgatgccg ccgtagtctt cccacgccga gcctttgacc gtcatgtggc
    24121 cttcgcggaa cggcgtcgag gtccattgga agacgttgcc cgccatgtcg agcacgccgt
    24181 acgggcttgc gccgtcggtg tattggccga ccggcgtcgt gtcacctgga cctttgacgg
    24241 cggagttgag cttggttggc tcgtagacgt tgccccacgg atagacgaga ccaccttcgc
    24301 cgcgcgtcgc cttttcgaat tcgtcggcgg tggggaggcg gcgtgcgtga ccggtgagct
    24361 cgccgcgcca cttgcagtac gcctcggcct ccttccatgt gacgagcacg accggatgat
    24421 cttcgcggcc gatcggtggc ccgccatctt tccagttgaa gcgttggacc gtcgcgaagt
    24481 cttggacgaa gccttgggcc gtccatgcct gctcgtcgat ggatgggggt ggcgcgcgct
    24541 tcgcgagcac gaactcggcg tactcggctt gcgtgaccgg catgagatcg atgcggaacg
    24601 cgtcgatgcc ggtttggtga cggtcttctt cggcgtcgaa ccatttgttc tcgcgcgcgg
    24661 tgtcgtgacc cgatgtcgcg aggtagtcgt cgtacgccga cgcgcgctct tctggcgtcg
    24721 agccggcgat gaagcgaccc gcggggatcg agatcatgtg cggatctttg gctaacgaca
    24781 gcggtgcggt cgacgtacct ggcttcgtgg gacccggcgt cgacgagcac gcgacgacga
    24841 acgggaggag cgaggcaagc gcgatacgaa tcatggagag ctggagggcg attgcgtggc
    24901 catggccgcg gccatgatgc gtgccagctc gggaaggtac tctggatcgg acgcgagtgc
    24961 ggcgctgtcg aggtcgcgac gcgcgatcgc gggagtgaga cgggcgaccg cgcccgatcg
    25021 atcggcgagc aggcgcgctt tgcgcggcga cgcgtgcgtg tcgatcacga tgacggacga
    25081 gaagaccgcg ttgccgttcg cgccgcgcag gatcgaagtt gcgaacaggt agcggccgtc
    25141 cggtgaccag acaggaccac tctcgtcggt gagcggcaga tcgatcacgc gcagcgcagc
    25201 atcgctgtgg accggcatcg tccatagctc gccgtcgaga ccggcctcgt gctcttgcgg
    25261 gcgcgagaac gcgatcgttc gatcgtcggg cgagagctgc ggagatgcgt cggccgggcc
    25321 ggcggtcagg ctcgtgatcg ttccgtcggg tgcacgcgct tcgaggtgcg agtcgatcga
    25381 gccatcggct ttgggcgtga ccgacgcgta gacgatcgtg ccgtcactcg cgatcgttgg
    25441 cgtgatctcg tgaccgtcgc cgcgcgtgag cggttgcggc tcgccggatg cgcggccgtc
    25501 ggcgatcgcg aggcggtaga gatcgaagtc gccgccatcg cgcgtcgacg caaacacgat
    25561 cgagcggcca tcgcgcgacc atgtcgggtg actgtcgatc gcgtcgccgg cggtgagccg
    25621 cgctggcact gcatcggcga cgaggcgcgc gatccagagg ctcgtcgtat cgagcgagcg
    25681 atcgcgcgaa gacgcgaaca cgatccattg gccatcgggc gagatcgcgg gatgcgagtc
    25741 gcgcgcgaga gcttcggatg cttgcagcag atcgaagcgg cgatcgccac gctcgtcgat
    25801 cgcgacgagg cgcgcgcctt gcggtccact ctccgacgcg atgatcgcga tcggaggcgg
    25861 agccttgcgc ggcgaggcaa caggtggcga accacccgag caactcgcca gcgctagaag
    25921 gagaagcgca ttactgcgca ggcgtcggtg caggcgccgg cgtgggtgcg ggcgcaggag
    25981 caggagcctc agtcttcttc gactttttgg ttaggttgtc catgttcatg cccttcgtca
    26041 tgtccttgtg gtcgggctcg acgcccgcat agacaccaag ggcaaggacg aggaaggcga
    26101 tgccggcgat gagaccgccc atccatgcct tgcgctgcga ctcttggccg tactgctcgt
    26161 agaccacaaa cgtttgctct tggtcccttg gccaggacga gaaatccatc atgttggcag
    26221 cctccgcact acgactcgaa cggtagaagg tatttgaaaa ccctgcaatg gaatcgaccg
    26281 gtcgtgtgtc tcgttcgttg agagaaggaa gcccagatag gcctccacaa ggtgctatga
    26341 cacccgacac acgagtcggt tcccgatttt gtttggtttt tgcgctagcg atcttcggtg
    26401 gttgcagcgc gcgtggtgaa gctcagcctg tctctcaggc gcctgccgca ccgcgcgatc
    26461 ccgcaactcc tggcgaaggt agcgcgagcc gcgacgccca caagggcgag agctgccttg
    26521 gcgtgaaaga cgccggcatc ttcggcgacc tcgacgacaa agtgcagctc tccctgcccg
    26581 cgggcctgac cgcagaacgc gtcagcgcga ccgtcgatcg caagcacaag ctgcttgtcc
    26641 tctcgatcga cgacttcccg cgcaaggtct acccgctgac cggcacggag acgctgaccg
    26701 tcggctcgat cgagctcgcg ctgcgggaag gtgatcgcga cgagctgaag ccagtgctca
    26761 ccgccgagcg cacgaaagag gcagcgcccg cgcgcgatcg cgacaaggac ggcatccccg
    26821 accctctcga tatcctcatc ggcgcgaaga agaccgtgct caacgccgat gcgtacaccg
    26881 agaaggcgca gagctacatc acgctgaagt atcccggcgg cgacgtcgat cgctcgatgg
    26941 gtgtgtgcac cgacgtcatc attcgctcgg tacgcaatgc cggcctcgat ctacagaagg
    27001 agctgcacga ggacatcctg aagcgcggca aagcggcgtt ccccatgatc cgcaaagccg
    27061 cagacgcgag catcgatcaa cgccgcgtcg gtacgctcct gccctacttc aagaagcact
    27121 gggagcagca cacggccaag ctcgatgatc cgtcggatcc gctgcggccg ggcgacatca
    27181 tcttgatgga cacgttcccg agccgttccg ggcctgacca catcggcatc atttcggatc
    27241 agctcgacga gaatggccta cctctcgtca tcaacaactg gacgaacggt acggtgactt
    27301 ctgagatgga tctgctggag ttcgtcccgg tgctgtatcg gttccggtta cccgcgtaga
    27361 accggcgccg ctcgcgttgt atctttggcg cgtgaagcgt gccctcctcg cggtcttgct
    27421 cgtcctcacg agcatgctcg caggaccctc gcgcgcgctc gccgatgacg aagccgcgaa
    27481 gtcgctgagc taccgcgcgg tggtcgatcg cgtcgagctc cagccttcgc cgctcggcgg
    27541 ccagcagctg cgcgtctttc tctccgcgct cgagatcggt ggcaagcagc tcgagctgtt
    27601 cgacccgcag tacaagctca agctctacgt cggctcgggc gagaagaagg ttccatactc
    27661 gctcggctcg tacgcgagca cggataccga tacggcgctc gtcgtgctcg tgcaggcatc
    27721 gctcgattac gccgaggcgc tccccgcgat cagcgatgcg ctcgacgccg cactgctgcc
    27781 ggcgctctcc acacacacgc aggtcgtcgt gctgccgttc ggcgagaccg cagcgacagg
    27841 caagctcggc tcggtcaagc aggcgatcgg caaagggcta tcgaccgaca actcggccgg
    27901 cgatcccgcg ctgctcgacg cgatcgaccg tgcgctccag atcgtgcgca aggcgaagac
    27961 ggagccggaa ggccggccgc tgcgcaagat gatcatcgtc atcggcgacg gtcgcgacat
    28021 gtcgggcgac aaggagcgcg tcacccgcac cggtactcgc gccgcgaaag acggagtgcg
    28081 cattcactgc atcgcgtttt catcggcgga tattcgccgg ccgatgctcg cgctcggtga
    28141 gctctcgaag cgatcgcttg gcacgtttcg ctgggtgcgc acgtcgagcg gcgaatcgtg
    28201 gaaggccgcg ctcgaacaac ttcgcgacga gatcaacaag cagtacgtcg tcacgtactt
    28261 cgtctcgccc gaggacgacg tcgccggcca caagatgcac atcgtgatcg cgggccgcat
    28321 cgacgcgaca tcgaacgagc tcaagattcc ggagacgcct gcgtgcggcg cgaacgaatg
    28381 caaagcggac tactgctccg ccgacaagtg cgttcgctat cagagcgacg gcgggcgcgg
    28441 aattctcggc tggctgctgc tcatcggcgg cagcgtcgtc ggtctcgtcg tgctgctcgg
    28501 cgtcgttggc ttcttcatcc aaaaggcgca gcaaccgaag atcgcgtacc cgccgggctt
    28561 ccagccaccg ccgggcacgc ccgctccaaa gcccgccaag caaaagaaga gcaagcaacc
    28621 gcagcagcct gcaggtccgc ctcctggctt tctgccgaac ggccgcccga tgcccgcgat
    28681 attgattacg agcggaccgc gcaccggcga gcgtcacatg ctccgcaacg gctatctcat
    28741 cggcaaacag cccggctgcg acctgatcat cgaggacggc tacacctcga gccaacacgc
    28801 gcagatcggg atggacccga acggcgtctg caagctctac gatcgaggct ctacgaacgg
    28861 cacgtacgtg cagggcgttc ggatcacgga gagcgcgctg caacacggca ccatgattcg
    28921 catcggttcg acggaaatga ggttcttggc cgaatgaagt acggcactat gaggatcgcg
    28981 cgatgaggaa acagacgggg aacctgatca ttgcgaacca gccgttcaat gtgaacgcac
    29041 cggtcatcaa ctttcgcgag ccaccgtact gggacgcgac gcgcgaggtc tgccaaccaa
    29101 cggcgacgaa cccaaatccg caatgcaagc caggcggcgt tccgtacggc aacctgcccc
    29161 atccatatac gaagcgctac gcgctgcgcg gtccgctacg ccgctacggc atgaacccgc
    29221 cgctcgaagc ggtgaaggct gtcatcaagc agttcgtcgt tcatcacgat ggttgcagca
    29281 gcagcgacat gtgcttctcc gtgctgcaga acgagcgcgg cctgtcgtgt cacttcctga
    29341 tcgataacga cggcacgatc tatcagacga tcgatctcgc gttcatggcc tatcacgcag
    29401 cggaatggaa cacggcgtcg atcggcgtcg agttctgcaa ccgcggcgac gcgaagaagg
    29461 aaccgaacta ctactcgagc ggcaagttcg gccccaagcg caacgtcaag ccggtcaaga
    29521 tcaacggcaa cacgatcctc gcgttcgagt acacgccggc gcagatcgat gcgttcactg
    29581 cgctcggcaa ggcgctcacg cgtcttctgc cgaacatcgc gacggagtat ccgcagtcga
    29641 gcccgggcca agcagcgtgg gagacgattc cgacgagcgc gtcgttctcg ttctccggct
    29701 acatcggcca ctaccacctg atcacgcaga agtgggatcc aggtccgttc gacttcaaga
    29761 agttctgcag cgacctgcgc ggctcgtttt gcttccccgt gttcccgaag ccgtcgcctg
    29821 accggaaacc ggacgacaag cccgcggtgc ccgaggacag cgacgcactc aaagaagcgg
    29881 cgagcgagct ctacaaggcg aacgagcagc gcgctgacgg tggctatttc cccgtgggcc
    29941 cgtggggcga agcgcgcctg tggcacggcg gtgttcacct gagcggcaag acgaacgatc
    30001 cggtcttcgc gccgttcccc ggccgcatgg tcgccgcgcg aatgggaccg agcacgccga
    30061 tcggctcgac gaacttcgtg ctgctcaagc acgacatgac gctcggcaag tcgcgcgtgc
    30121 agttctactc gctgtacatg catctcgccg acgagaagaa cgccgagaag ccgctcgagt
    30181 ggacgcagaa gagcgagtcg tggaaggccg cgaagggtgg cgacgtcgtg ctgctcgacg
    30241 agccgatcgc tgcaggtact ctgatcggtc acgtcggcac cgcgggtcct gcggaatggt
    30301 cgaaggcgca agtccacgtc gagttcttct cgaacagcga gctgtttacc gacatcccgg
    30361 gctcgccgtg gcaattcgtc gacggcaccg caggcggtcg gttctgcgac gtgaagacgc
    30421 tcaacgacat gttcgacgac gacaaggacg gcacgctgtc gcggcaagag ctgtcgagct
    30481 tctactcggg cggacgcgca tccggccttc gctacacggt cgtgctgcac gtcagcgagt
    30541 ggacggccga gccgagctgg gccgacgcgc tgaaggttcc gaaggacttc aagaagctaa
    30601 agcccgccga catcgatcag ctcgtcgccg atcagatcac gccgggactg tggtgggacg
    30661 cgcgcgtcgc gggccatgcg cggctccccg ccgacggcgt cgtgttccac taccacccgg
    30721 tgacgttcct cgcgtggttc aaccagcagc tgctcgacgc aaaggcgaac gcaggtcccg
    30781 aagcgagcgc gacggatgcg caggaagtgc ccaagggcat caccgacgac ttcggcgata
    30841 tcaacggctc gtcgatgcgc tcgtcggcgg atatgtccga cgacccgtgc aaccagaaga
    30901 tcacgctcgc cgacctcgta cagggcttcg acgcgccgga ttgcacgcca tgaagaagca
    30961 ggacgacgag acgaagacga cgtggcgccc gccgacgagg cgcgcgttca ttgcgatgat
    31021 cggtgcggcg ggtgcagcgc tcgcgcttcg cgagaaacgg cgccgcgcgg gggatccgcc
    31081 ccggtctcct cagaccggca agaccctctg gatcggccac tgctaacctg gaaacaggga
    31141 aattccctgt gtatcgtgcg cgcatgtccc tgcgtcgctt gattgctgtg ttgagcgtgt
    31201 tggtgatcgc gtccggttcg gccgcgctcg cgcagactcc agccccggca actcctcccg
    31261 caggaaccgg ctctggctct gggtccggat cgggcagcgc gcccgccgac ggctctgcag
    31321 ttcagccgat cgaagacgcg ccgccgtccg acatggaagg ccgcgacgaa aatcccgatg
    31381 cgccgcacgg caccgacgac ggcacgactg ccgtggtcgc cacgcccaag gcgatgaagc
    31441 ccgccggcta tcccctcgag gagagccagc ggccgatcac gttgcccgcg aacatggccg
    31501 aaatttcgat cggcccgcac ttccaagcgt cgccgtacgc cggcgctgac gcactccgcg
    31561 cgcgctacgg catcacgaag cagatccagt tcggcctgac gtacgtgatg ggcggcgtgt
    31621 acgacgatcc ggtcaactcg aagaaggaat acaagttcca cgtcggtaag gccgcgggtg
    31681 tcgacgtcac ggtgctgctc acgaacttcc tcgcggttcg cgtgggcgtc cctgtgtact
    31741 tcgatccgtt tgcggcatcg tttgccgcgg gcgcgccgat gaagttcatc ctcaacgaca
    31801 agctcgcgat cggcggcatg gaagacgtcc tgaacatccg catcaagcgc ttcgcgccga
    31861 gcttctacca agaggcctac aacgccggcg gcgccgcgaa cgacatgaac aacaccgaac
    31921 agtcccgtgg gcgcctgcgg ttctcggggt ttgcggtcta ccagcagaat ccgaagacgg
    31981 cgctcctcgg caaggtcggt ctcgagacgg acctcggagc cggcggcggt ggcgctgcgg
    32041 gcacgtcgac gccgagcgac accgtcacgt tcatccgtgc gggagttcaa tactccccga
    32101 agaagtacct cgatgtgggt gggacgatcg gattcgacga cctgagcaag ctcggctcgt
    32161 tcggcgtagc gggaattctc gcagtccgaa tctgatctct tggtacccga gcggtaccca
    32221 ttctccgtcg cacggcgctg atctgtaacg gtgttccgtg tagcatggct tacatggggt
    32281 tacgtaggct ttgcttccta ctgtcggtga cgtctgtctt gatgtttgcc gcagcatgcg
    32341 gcgacaacaa gcatgtcgat cgcgatgacg cgggtaccga cggaggcagc gacgcttcga
    32401 tcgatgcacc gcccgacgcg ctgatgtgca cgtcgcccca gatcaagtgc ggcgacgcat
    32461 gcgtgaaccc tacgacggac aacgcgaact gcggcgcatg cggcgtcacg tgcagcggca
    32521 ccgactcgtg ctcgaccgac ggctcctcga cgaagtgttg tcctgcgggt cagcacaact
    32581 gtggcggcgt ctgcgtcgac tggtcgagcg acgaagcgca ctgcggctcg tgcgcgaacg
    32641 cgtgcacgtc gggtgacact tgcgataacg gcacgtgctg cggctcgaac acgacggtct
    32701 gcacgacggg ccaagggccg cagtgcttcg acctcaagac cgacgagatg cactgcggta
    32761 cgtgcacgac gacatgcacc aacggtcaga cgtgcaccac gcgcaccgac aacacggatc
    32821 cgaagtgctg cacgacgggc cagaacaact gcggcggcac ctgctccgat ccgcagaccg
    32881 acagcgcgaa ctgcggtgca tgcggaacga actgcggcgc cggcaactcg tgctcggctg
    32941 gcaagtgctg cccgaacggc cagacgaact gcaacggcac ctgcgtcgat ctcaagaccg
    33001 acgaagatca ctgcggctcg tgcccgacgg cgtgtgctga caacgtcgag acgtgcacga
    33061 cacgcacgga cggcaccgat ccgaagtgct gcgcgaacaa ctacaacaac tgcggcggca
    33121 cctgctcgaa cccgctcacc gacaacctga actgcggctc gtgcggtaac gcatgcggtg
    33181 cgggtcagtc gtgctcgaac ggcatctgct gcggccccgg ccaaacggca tgcggcaaca
    33241 cgtgcgtcga tctcaacacc gacaacacgc actgcggcac gtgcagcacg aactgcagca
    33301 cgacggcgaa cccggtgtgc agcggcggtc agtgcgcgtc ggcgtgcccg gccggcgaga
    33361 ccaactgcag cggcgactgc gtcttcacgc tcaccgacca cgatcattgc ggcagctgca
    33421 cgaacgcctg cggcccgtcg caggtctgca acggcggcac gtgtacgacg acgtgtccga
    33481 gcggcacgat ggcgtgcggc ggctcgtgta tcgacgtgct cacggatccg aaccactgcg
    33541 gtaactgcgg cacggtctgc ggtccgacgg acgagtgcgt caacggtagc tgccagctcg
    33601 actgccccac cggccaagtt ctgtgcggcg gcgtctgcgt gaacccgctc aacgacaaca
    33661 accattgctc gacgaatacg agctcgtgcg gcgtggcgtg cggctcgggc aatacgtgcg
    33721 agctcggcgt ttgctgccag aacggttgga agaactgcaa cggcgtgtgc gtcaacccgg
    33781 cgtcgaacgc gaactgtggc gagccaggct cgaacaactg cggcgtgaac tgcggatcca
    33841 ccggcgagca atgcaagtcg agcggctcgt cgcacgtctg ctgcgcgtcg ggcgagatca
    33901 actgcaacgg cacatgcgcc gatatcacga gcgacgaggc gaactgcggc gcgtgcgccg
    33961 gcggtggtgg tcagacatgc ggtagcggcc agacgtgctc gaacggcgag tgctgcaacg
    34021 gcacgctcgt caatctgaac ggcatctgct gcgcgaaccc ggcgacgacg cccggcagcg
    34081 cagcctgcgg tactccgccc acctgcacga acacgcgcag cgacgacaac aactgcggcg
    34141 cctgcggcac ggtgtgcgcg tcgaacgaga actgcgtcaa tggttcatgc accacgcagt
    34201 gcctctcgcc aaacgcgctc gcgtgtaacc cgcagatctg tacgaacacg cagaccgatc
    34261 cgaaccattg cggcgccgcg tgcgcgcagt gtccgacgcc tgcaacctgc aacgcgggct
    34321 cgtgccagtc gtgcacggtg gccaccggcg gtcagttccc cgatgtctgc aacgacgcct
    34381 gcacgaacaa gctgacggat ccgaacaact gcggtgcgtg cgacacgttc tgcggcagca
    34441 acgagcaatg cagcaccggc ctctgctgct cgatcggcac cacgcactgc ggcagtggtt
    34501 gcgtcgactt caccgctggc gttccgcagt ccgatggcac gactgcgaac tgcggcgggt
    34561 gcggcccgtc gttcacctgt gcggctggct cgacgtgtac gaacggccag tgcaagtgcc
    34621 cctttggcac cgagccgtgt aacggcggat gcgtgcagac cgccacggac cctgacaact
    34681 gtggagcctg cggcaagaac tgcacgggca ccgacgcgtg cgtgtcgggc agctgcgtag
    34741 cgacgtgccc ggcgccgctc atcaagtgcg gcgatcaatg cgtcaatccc gacgacgacg
    34801 acgcgcactg cggtggctgc accggcaact gcaacttcgc ctcgaccggc aagcgctgct
    34861 cggcgggcgc atgcgtgaac ccgacctggc ctccgttcgg tgtcactccg acgttcccag
    34921 cgaagtgcgc aggtggtggt cccccgatcg ttgtcgatcc gggcgccggc aacatctgca
    34981 ccggtaacct cggtcaggtc gcgtttacgt tcggtctctg ctcgcgcacc gcgatcggtc
    35041 cgttgagcca agacctgtat accgatgcat tcgacagcac gtcgggcgcg tacacggcga
    35101 gctgcctcac cggcggcgtc gacgacaact cgaagtgcgc gtcgctccac tgcgtgaaga
    35161 gcctcgaccc atgcacgagc acgacaccgg gtgtcggccc gggtaacggt gcgtcgcctt
    35221 gcgcgaccgg tgacgtctgc gcgtacccgg tgaagtgcgt cggcggcacg tgcatcggcg
    35281 gcggcgtcgg cgtgaacggc accgctacgc ctgtcgcctc gaacaccgcg aacacgcacg
    35341 tcggtggtga cttctggatc tacggcacga cgggcctcgc gatgaagggc aacacccagg
    35401 tcaagggcat cctcgtgaac cagaactcga tcgacatcgg caagacgtcg acggtctatg
    35461 gcaagtcatt cgtcggcggt ccatggacgt cgggcggcaa tacctcgatg accctccgct
    35521 caaatctgtt caccgaggtc gcgtgcaacc aagtgcccgg cagcgtcacg ttcacgaacg
    35581 gcgcgacctg taactcggtg acgaactggt tcggcaaccc gggctacggc gcgggaagca
    35641 cgaatccgac ggagccgaac ccaccgtgcg gtctcgataa cggccaggac ggctcgctca
    35701 tcaacatcaa gaagatcgtc accgattact cgagcagcgc gaagaacgac aacttcaacg
    35761 tcacgccgaa catcccgtac agcctgttcg acgccgtgtc ggcgaccgtt cgtgtcgacc
    35821 tgccctgcgg ctacttctac ttcaaccaga tgaacatcgg taaggacacg acgatcgtcg
    35881 tgcatggccg cacggcgatc ttcgtcgcgg gcgcgatgcg cgtgagccag aagctcatcc
    35941 tcgatctcga tccgacggcg acgctcgaca tcttcgtcgg tggcgcggtc aacgtgtcga
    36001 acatcgtcga gctcggctcg ccggcgttcc cgcgccggtc gcgtctgtgg atcggtggcg
    36061 ccggctgcct cggtaccggc acgtgtagct cgaacagcga ttgctgctcg ggtatctgca
    36121 ccagcggtgt gtgtgcgtcg ggcggtggtg gcaacctgtc gcaagcgatg tcgctctcga
    36181 acggcggctt cttcaacggc ctcatctggg gcggttgggg aacgttcacg cactcgaacc
    36241 cgctgtcgat gtacggctcg atctacacga actactacga cgcgtcgggt gacacgaaca
    36301 tccactacga cctcggcgcg acgaagatcg gcgaggagtg cccgccgccg cctgcgggtg
    36361 gcctgtgcga cagctgccgc cagtgcggca atcagccgtg cgtcaacaac cactgcggtc
    36421 aggcgtgtac gtcggacagc cagtgctgtg cgccgctgaa gtgcgacctg ggctcgggta
    36481 tctgtctctg agctagtcgg ctggcttcgc gtcggtcacg ccggctccag catcgggagt
    36541 cggcgttgtt gttttcgggc tcgcgcttcc ggtgtcggca ggcgcgtgct cgccggtggc
    36601 cgtgagcacc tcgaacttgc gaagaccgac gagctggccg cccttcgtat acgtctcgca
    36661 cgaccacttg cccgtcggat cggcgggtac gcgatccttc ggcaagtagc tgcgaacgac
    36721 gatcgcgtcg ccatcgtcgc aggccatcac ctccggctgg atcggcgtgc cgagcgcgtg
    36781 accgcgatac gtccacacgt ggacgacggg ctccttcatg ccaccgggct cgcgcagcat
    36841 cgagccgcag cgcaggccgt cgagctgcgt tgcgcgaatg gcgtgcttac tcggcggcag
    36901 acactcgtag ctgccgagcg tgccgtggcc gaccatcgtc tccgacatcg cgagtggcgc
    36961 gggcgggatg aaccgcttgc cgtacgacac cgcagcgagg agcacgcccg tcgtaccgag
    37021 cgtgagcaag gcgccttgcg gcgagagcac ctgccggatc gagaacgaga gaaatgccac
    37081 ggacgcgggt gtcgccgctg ccgcgatcac gaggcccgtg aagtgatcga tgccgagcag
    37141 cagcggcagc atcacgttga gcattccgaa catcgcgagg ccgtacatcc ccgatgcgag
    37201 gaaccgatgc tccatgacga agttgtcaaa gacgagatcc atcgtcgaga gcaccgcgca
    37261 cacgagcagc agtggcgcga gccaccagtt ccacgatgcg aacgaccagg tcgcgctcga
    37321 cgcgtagagc ggcgtcagga agaagaacat ctgctggtag aactgcttga tgatgtagtt
    37381 cgtcgcgacg cggatgccct tgcggatgag cttctcgtcc tcgcgtcgat tcgtgggacc
    37441 ggtgatgaaa cgaaacgcga tgaacatcag cagccagctg ccgaggagcg cgaccatcac
    37501 cttgtcggcg tacttgaggc ccgcgcgcgc gaacagcatc acgccgaagc cgaacgacag
    37561 cgcgaagccg gagtgcagcc accacagctt gcgcacgacc cagcggatcc gcgacggcgc
    37621 gggtgcctct gctgcgggag cggcctcttc ttcgtccgcg gcgttcgcca cgaacggctt
    37681 atagccgaat cacgaggcgc tagcgatcag ctctttgaat cgctcgagcg ccaggtcgag
    37741 cttggccatc gccggcccaa acgagaagcg caggtgacga cggaagcgcg aggcgcgtcc
    37801 accgcggcgc ttgcccggat caacgtcgaa gaattcgccc ggcaccgtga tgaccttgcg
    37861 atcgagcgcg gcacggaaga acgacatgcc atcgctaatc gacgcaggca gatgctgcgc
    37921 tgacgcccag acgtagaacg tgccctcggg tggcagatcg accgtgaagc cgaggtcgcg
    37981 caggccgttg agcaggcgcg agcgcttctt gccgaactcg gtgtgaatcg cgcgggtctc
    38041 tgccatcgcc gcggtgggat gcagcagatc gatcgccttg cgttgaagcg ggcgcgagcc
    38101 gccaccgtcg aggaacgagc ctgcgctcgt gacgccctcg atgatcttct tgggcgcaac
    38161 ggtccacgtc acgcgccaac cgggataacg ccaattcttg gtgagcccgt cgaggatcac
    38221 gacaggatcg cgatcgacat cctgcacgta gcgcgcagcc gtctcgatcg caccttgcgc
    38281 gacgagatcc ggacgccaga tgtagtgcga gtagaactcg tcgaaaatga gcgcgcagtc
    38341 gagctcgcgt cccacgctga cccactccga aagatcgccg ccgccaacaa ccttgccggt
    38401 cgggttcgaa ggattgctcg cgaggatcgc cgacaggccg cgcccgagca cttcgcgtcg
    38461 aagatccgcg cccgagaatg cgtagcctcg ctcgggctcg agcaggatcg gaatcggcgc
    38521 gaacagacga aacacgctga gcagctcttc gtacgcggtg tagtccggca ggaagtggcc
    38581 gaggttgatg ttgccaagcg atgccgcgac gcgcgtgagc gccgaccgac caccgcccga
    38641 taccgcgacg ttctccgccg agtacttgct gcccattcct cgacggaaca gatcgttgta
    38701 gtagcccgca atcgcttcgc gcagctccca caatccagcg acgggcgcgt actcctgatc
    38761 gcctggctcg atcggcaggt cttgaatgcg cggaggcgcg ccgggcagcg cctcggcctc
    38821 gggcatgccc tgccccaagt tcgcccagtc ctccgcggtc tgcgaataac cgcgtcgcga
    38881 tgcttcggtc gtcacgtaga tgacgccggt gcgcggcacc tcgcgaaatg ccgcgaactg
    38941 cggcggcgtc tcaccacctt gcggacccgt acccgttttc ggacgcggaa actgcgacat
    39001 gcgaggagac attagcaccg cgccgatcgt cctcccagct aatggcctat ctgtgccgca
    39061 ccctgcgctt tccaatcagc gccgcagtag gcccgagtga acttcgccgc aatttccttg
    39121 gtgatcgtca cgtcgcagat cggccgatcg gtgcccgcgc aggggcagta atcgtcggca
    39181 ttgaatgcgc gccacttgtc gagcacgagc ccatcgccgc tcttcgcgat gagctgcacg
    39241 tgcgtatcga gcggcagata cgcgctgcac gaaacgccgc cgcctccgct cgtgacgact
    39301 ttcgggaaga gctccttccc cgtcgcgacg tcgacgccgt gaacgctcgt cgacttgccg
    39361 atcgagcatc cgttgcacgc ggtgttgcag cccgcgatca cgtaggtgcc gggtggcgac
    39421 gctacaggtg gattcggcgg cggcggatga gcgtcgctct cgcgctccca ctgatcgcgc
    39481 tgcaccgtct tctgcgcttc ggtctgcatt tgctcatcgg gattcttcgg ctcggtgttc
    39541 cgccagcagc tcgcgagcgc gacgagggcg acgagcgcgc cgagcgcgat gaccgactta
    39601 cgaaggcagc ggctcgaggt ggttgcgcag cggctcgagg tggttgcgca gcggctcgcg
    39661 atggatgccc tcacggcttg ccggcggcgt tgcatgcctt cggatccttg cgacagtcac
    39721 tcttgatgat cggatcgttg ccgagctttc ccttcggagg cggcaccttc ttcttgtccg
    39781 acggcgcgaa cggatcgacg tgcatcggcg gcggtcgcgg cccctgccct ttgcgcgggc
    39841 tgccgtcgag cgggacaacg tcgtcatcgc tgatcggatc gggcgttgcc gcggtcgcgg
    39901 tggtcgtcgc ggtcgtcgcg gtggtcgagg acgtcgccgt ggacgcggtc gactgcggcg
    39961 gcgaggtggt cgctgtggtc gcggtggtcg ctgtggtcgc gctggtcgca gtggtcgcgg
    40021 gcgtctgccg tgcagcgggc tcggccgagt gctgactcgt cgaaccgcag ccggcgacaa
    40081 gcgcggccac cgccaccgtc cctgcgatca gccgtcgcct catatcccca tgaacgcacg
    40141 gcgtgccgtc gagatccagg gcggatcgcg tgtgcgatcg gatggttgtg ctagcttggg
    40201 gccgtgtcga tctgggacgg gattccccag caagaacgcg tcgtagggct gctccgcggg
    40261 gcgatcgaac gctcgcgcgt tccgcatgcc tatctcttca gtgggccggt cggcgcaccg
    40321 cttctcgaca cggccacggc gctcgctgtc gcgctcaact gccaagtcgc ccgcggcgaa
    40381 ggctgcggcg aatgcgactc ctgctcgaag atcatcggtg gcatccatcc cgacgtcgtg
    40441 acgctcgtac gcgaaggcgc cgccaacatc gtgccgatcg agagcgtgcg caaccaagtc
    40501 atcgcgcgca tcggtctccc cccgcacgaa gccgacgtcc gcgtattcat cgtcgaagaa
    40561 gccactgcga tggcaccgcc cgcggccaac gcgctgctga aaacgctcga agaaccgccg
    40621 ccgcgcacgc tcttcatcct gctcacgacc gcgcccgagc agctcctccc cacgattcgc
    40681 agtcgctgcc aacgcgttcg cttcggcggt ggcgcgggcc tcgtcgacgc agatccatcg
    40741 cgcgccgaac gcatctcgaa gctgggcgaa gagctcgccg gcgatcgcca cgatccgacg
    40801 ctgcctaacc gcgttgccga aggcaaaggt gacgcggcgc ccgtcctcgt cgcggccgca
    40861 ctccgcctcc acgcgctcgc ccacgaggct gccgcacgcg ccgacatcgc cgccgcaaaa
    40921 cgcgcctcgc gtcgcgcgca aacggtgctc tcgtggcaca ccgccgtcgc gatccataac
    40981 gccaacgcgc agctcgcgat cgaagcgctg atcgcgcagc tcgcctcgca ggatcgcgtg
    41041 tcgtgagccc accgcccgac gacgaagacg gccccgacga cggtccggac gacggctccg
    41101 acggtggcga tgaaggtgcg gacgatgcga tcggcacagg cgtagattcc gcggtcgcag
    41161 gtggtggccc cggcggtcca tcgtcgctcg gcagtcccga ccccgcacgc aaacgccgtc
    41221 gacgccgccg tcgtcgtcgc gatcacgcag gcgatccagc cgacgcaccg cgcatccccg
    41281 accaagccga cgcaccgacg cgcgaacagc tcgccgccga aacgccgcag gacggctcgc
    41341 agccgccgcg cgacgccact gccgcgcaac ctcgcggcga cagttcgcaa gcaggctccg
    41401 cgcactcgca gtccggctcg ctgaccggat cgcagcccgg atcgcagcaa ggtcgtcgca
    41461 acgaccgacg cgacgcgcgc gacaaccgcg gtggcgatcg gcgcgacgcg cgcgacaacc
    41521 gcggtggcgg agggcgtccg ggtggcggac acggcggcgg cgatcggccg gctggcggac
    41581 acggcgccgc cgatcgtccg ggtgccggac acggcggtgg cgatcgtccg ggtgccggac
    41641 acggcggtcg cgatcggccg ggtggcggac accgcggcgg cgatcggccg ggtggcggac
    41701 acagcggcgg cgatcggccg ggtgccg
//
LOCUS       DATLBZ010000317        23335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9870293, whole genome shotgun sequence.
ACCESSION   DATLBZ010000317 DATLBZ010000000
VERSION     DATLBZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 23335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23335
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9870293"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2865)
                     /locus_tag="VMZ53_16555"
     CDS             complement(<1..2865)
                     /locus_tag="VMZ53_16555"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30121.1"
                     /translation="MVDAVRADVSVPQRFAIVRELGEGGMGKVYEARDQELAIHVALK
                     ILHEGDGEAIEKLKHEFRVAADVRHENLVKLGELFEHGQRWCFSMELVNGVDVITHVA
                     RRPRVNVSAEAPTAPRGLRLPTADESGEKSWTDEANFDETKLRKTIGQLAAGLAALHA
                     SGIVHRDVKPSNIRVAPDGRVVLLDLGLAAAAIDLSDATQLAGTVPYMAPEQTESGIV
                     STAADLYAMGAVLYEALVGRPPFVGPNVVVIVEKTTSDPIAPSLLFPEVPPDLDALCM
                     ELLSRDPAKRPPAEDVARRIAAAEPAQRSSEPAVVGAAVRPARAFVGREQELKALLRA
                     AERSRKAPSVRVVTGASGIGKTRLLGRFTQACRDYGLFVCLGRCRVREHVRLNAWEAL
                     LDGLTRMLSSIPRAEATELLPPNAEAISRLFPIFARVLPVSTSSSLPAAELEHRAIVA
                     LRELLRRIALKMNVVIVIDDVQWATEASLELFAQLLEPPEAPPIALVFGLRTTEASTE
                     AITNWLGRVSEGDLDLSMMALGPLEPNEARSLAQLLVRDRDRADKIAKETEGHPLFLE
                     LLASTSQGSDALHRTMRRTIKDLAPPLRELLGVVAVSAAPLRIDLLGAALEREPFVLV
                     DELRGLLAQRLVSVTGLRRSDHVEPFHAQVAEATLALLSPDELRANHVRLAKGLESMP
                     FLQPERRALHWAWAGDAERAAKEVIALVANARHALAYSRAADVCEVVNALALSATSKS
                     TLSRAYADALAGSGHASRAAFAYRDAAETAAGTERLDLERRAAENFLRSGSLAEGMEI
                     AGRVAKQLGYAAQASPAKAIAQLLVERARLRLRGLEPRRGDVDSAIAAKSDACYSLST
                     GLAMVDTITGAVFQTRSTRFALESGDPARAARALAIEACFVAAWGSSTRDRAGRIVQA
                     AEKLAAEVGDPVVVAFARSARGICELQWGDFTAAIESCDKA"
     gene            2898..6026
                     /locus_tag="VMZ53_16560"
     CDS             2898..6026
                     /locus_tag="VMZ53_16560"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30122.1"
                     /translation="MSQPLDTSQTSGIRHGSKVGRFVVEGQLGAGGMGVVYAAHDREL
                     DRRVALKVLKGTDDAEQRTRLMREGQAMARVTHENVITVHEVGMEGSMVFLAQELLDG
                     GSLRDWLLADKKRSQSEILAKFIAAGRGLAAAHRAGLVHRDFKPDNVLLGKDGRVRVS
                     DFGLARSLESSAAALAETQKGGGSTDENLANPMLTMTRTGAVMGTPLYMSPEQHAGEP
                     ADERSDQFSFCVALYEALHGDTPFPGKTAVALADAVMSGRMKPPPKTADVPSRIRKIL
                     LRGLSTIPGERYPSMDALLADLTHDPARKRTRFVLLAAVIILIAGAVVGGYALSSHES
                     RKASEVPARRTVAVLGFKNTAADPASAWISQGMSQLLAAQLLADDVKVVSAEEALRAR
                     SELHLPDTDTYSADTLNKIRERLGADAVIGGSYFVSNGELTLAISVQDIPRKKSIPIQ
                     LKGTTAELPALAEKAGGEIRTALGISSVAIVKKSYDVLPHDAVAQREFIEGNEALRKF
                     DFRNAKMHLLAAVKAEPDFAPSHLALAKAYSGLFDADAARASAKQALATASSVGGDQQ
                     LVVRRDAYKLLDQIVEAREQANQLFVLHRDNLEYGLALAELQEPDDAVRTLATLRKLA
                     APAGTDPRIDIAEGRAELERQAAPRALELARHATQEATARNAAGVAAEARQLEGEVLV
                     ATGELATAQDTFEDARKRFEAANDQIHKLQTLESLADLSLERGQLDDALKSYDALASL
                     REQAGQTGVAARATARAAYALALAGKNADAEKRLARAQKMAADDPVAIPTIDLVAGVL
                     GWAKGDGPTALGRLDKCNARFASVAPGSSVLCLLLRGEIQAELGDAAAAKKTFDDAKA
                     VADKAGNVYRSQQAELRLAQLELDEESPADEEAKIADRIVERAGAIRIAAATHGATSL
                     EARAGIMLARAHLAQGNSQDALDVIAKVQKQEDIRIEVDRLITDALSKDAAGDATSRD
                     TLDEVKKIADKQGCVTLQLEARLALAEVLATSKEPGETEKGRAELATIQQEAVAKNLG
                     RIAKRAEILSRP"
     gene            6044..6970
                     /locus_tag="VMZ53_16565"
     CDS             6044..6970
                     /locus_tag="VMZ53_16565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30123.1"
                     /translation="MADCQLCGSHHKEDICPHARTGQVINNYVVGPLLGVGGIAAVYS
                     ARHLKLGREIAMKILHQRWAKDHELSSRFVREGKATAAVGHPAFVTVFDAGTTEDGCA
                     YIEMDKLVGRDLYAVRTQDGPLAVDRVISIAIQVLDGLSVLHSRGMIHRDLKSSNIYL
                     TPSEMGERVVLLDLGFAKLDDGLALTSNDQMLGTPFYISPEQYLDPTQVDARADLFSL
                     GIVMWEVLVGDFPYEWENKRDLLAKVMHGKLERHPAVRRPDVPAWLDEIIATALAHKR
                     EDRFESAAVMKAALDGKELPEKPGFLKRMFGR"
     gene            complement(6967..7518)
                     /locus_tag="VMZ53_16570"
     CDS             complement(6967..7518)
                     /locus_tag="VMZ53_16570"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HUS30124.1"
                     /translation="MPGKVGSVPIYVALLRGINVGGSRPVKMQALRATFARVGCTNVA
                     TYIQSGNVVFAHAEKSEAKLVALVEAAIAKDAKFDVKVVVRTKAELAKIVAKNPFKKA
                     TDQQLHVFFMAKKPARDALAKVDAKAFEPEKFAIVDRELYVYLPNGLGRSKLAGTLMR
                     KPPIDEATARNWRTVQTLVAMTG"
     gene            7569..8237
                     /locus_tag="VMZ53_16575"
     CDS             7569..8237
                     /locus_tag="VMZ53_16575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUS30125.1"
                     /translation="MLAFVPLISAAAGGLAYLACYGGIAGSHGPRDKRRIALTFDDGP
                     DPLRTPALLDALAALDVPATFFLVGRAVDAHPELAARIAREGHEIGNHTYSHRYLPLA
                     RSRSVAAELAATDAAIARATGIVPVLARPPYGGRSHRNVRAFARSEKRLVTWDVNSFD
                     WKGKPADVVVRRVLERTRPGSIVLMHEAREGGEVTIEAVRQLVPELRQQGFELTTVSR
                     TLGM"
     gene            8257..9777
                     /locus_tag="VMZ53_16580"
     CDS             8257..9777
                     /locus_tag="VMZ53_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30126.1"
                     /translation="MAGGDDDGEQATHVEGRFSRRMTTVKPPPAARLEDRGEIARGGQ
                     GVIRKMFDHNLHRYVAQKVMDPELLHHPDQRGRFLDEARITGQLDHPNIVPIYDVVEE
                     EHSTEFVMKLVEGETLSKRLKRRKSADDLPRFLEIFLKVCDAIAFAHSRGVIHRDLKP
                     ANIMIGEFGEVYVMDWGCAHILPRKGQLSENTPQRMIVDEPGTVIGTSAYMSPEQAHG
                     RTDEIDERTDVFGLGAVLYEILTGSPPYPGSNDDQQSALARAGKVRPPEEVEKLRTPP
                     PGVSAIAMRALSAKREDRQQTVVALRDEIERFLHRGAFFTPRPFPAGTLIVREGDIAD
                     EAYIITSGHCEAFREERGRKLSLRTLGPGEVFGEGALFAREPRNASIVAVDDVTAIVL
                     TQQVLDRELGTDSWLGTFIRALTARYRDLEARDMLTRRVIENGRIAAEIINHISRAGR
                     WVRPGVLGTSWSRVWSALQPVLHITEDQALSIVAGTQDLDYDAPRDEITLALLSMA"
     gene            9780..10259
                     /gene="ybaK"
                     /locus_tag="VMZ53_16585"
     CDS             9780..10259
                     /gene="ybaK"
                     /locus_tag="VMZ53_16585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HUS30127.1"
                     /translation="MSKHPVTMAIRALRTGKVDFVPHLYAWQPHGGTRASAEAFGVDE
                     HIVIKTLIFEDDAKKPLCILMHGDREVSAKQLARVIGAKTVSPCTPEIADRHSGYQVG
                     GTSPFGLRKQMPVYMERTIAELPRIYINGGARGFLVEIDPKDAIRVLSPTLVDVATT"
     gene            10306..10680
                     /locus_tag="VMZ53_16590"
     CDS             10306..10680
                     /locus_tag="VMZ53_16590"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HUS30128.1"
                     /translation="MRSKISALLFVVFAATGVTARADVVCRDGTSSVASGRGACSHHG
                     GVAKKRAERTRAPKRADDVVLEPREQSRTDRIRRRGERSHPIEKRESGSPTAVCRDGK
                     LSYALHHRGACSGHDGVERWLD"
     gene            complement(10677..12848)
                     /locus_tag="VMZ53_16595"
     CDS             complement(10677..12848)
                     /locus_tag="VMZ53_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS30129.1"
                     /translation="MLRVWLLLAITVSVVEAAPYQHVSGVVIDKATGEPVEGALVVGA
                     NVSETTSVTGTFTIMLAPDEVEVLVTAPGYAMRVVKLTSTSLRIELESASGAEVIDVE
                     GRLPVQPAAKKYELTSDDLRSLPGTANDVLRAAQALPGVSRLPFSFGGIVLRGTSPRD
                     NAVYLDGIEVPLAFHFGGITSFYPSNMLSGLSVSNGGLEASYGRAQGGIVSLTTREPR
                     RDRWRKGGSIGLLDASAIVEGPFRSGSIAIGLRRSYFDIVAGPVAPDDVPLPSYWDAQ
                     VRMSFGDPKKEGGHIAPMAFLSLDDIYASQPGKDPQFEDESSMRAFFVRVAMPYKREL
                     EHQTIEIVPWLGTNQLTFGSVTNGVQEKFTRPVYTAGTRATLSHDYPAGTLRTGLDIQ
                     AGHLSRYQAGFGHRGDLISLVNGDTTVNWMDAAIWSDFRFTLAERISLRPGLRLEHYG
                     LTGEAVIDPRLAIQEKLTKELTFRETIGRYHQPPTPGDVDPNGGNPELTSSYVDAASI
                     GIERELPEGWTGSFTGFYNIGRDLGVRTMLDQKNFANLDGLGPTFELLLEKQLGLAFV
                     RSNIGRARSKGLELMIKHSSKTWFGMLSYTLSESERTDDPEIRAGWRPFELDQRHNLN
                     VAASIPLDKWRVGARIHLVSGTPLPAPLTGNLPVFFQLDVRADRKWPQCWGDVNFYAD
                     IQNITNRRNVEGRTFDVDSLLIKESRGLPIAPFIGVEMIPN"
     gene            complement(12858..13670)
                     /locus_tag="VMZ53_16600"
     CDS             complement(12858..13670)
                     /locus_tag="VMZ53_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30130.1"
                     /translation="MPFLAPLWLPFGLLDRQTLSPPRISDTIEINGGPAVTSTLHAQM
                     STQCDCHIGAYGTLPLAISVSEDPGVAAAGQAIPSDDSRVSIGTSEVGIFGGSERGRD
                     QMIYRLGVLLPSASREQAPLPVSARVGDAVLELPRTMGVRMSHSQIWIKDAPWTSVAK
                     HIMRLDVGLDVAGEYATEDHRRIVHMIPRIGTGVMQQCDLGSVSFETALSYDPFVEDG
                     GLRWSAGITGMLKSRWLQPAATLAAIRTPDGWGATLSLDLAATLQPRYAEGL"
     gene            13785..14612
                     /locus_tag="VMZ53_16605"
     CDS             13785..14612
                     /locus_tag="VMZ53_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30131.1"
                     /translation="MNIDDIKPANCYARARRLLGEVSLVRDELGRSEDTRQVPEITGA
                     QPRECYFEAIAAWRKAERLADELGVHKSRALPRTPTLRDIRPGHVYQVLEGVLHTLGE
                     VKSRLDIAESVVEPGVEPSRQPSDVLATIIRINRELSRCLERPFTPTDVYETVALASA
                     YASQLGATAELAPFERKRKPQHCYEMLASCLTAAASHVTQKGEQALVKRGMPVDVLPG
                     DVYDLAILVLGELAFLHSLTPNAATVQPFTPVGRGHRIPSDCYQLARTLEKQLTSLK"
     gene            14609..15088
                     /locus_tag="VMZ53_16610"
     CDS             14609..15088
                     /locus_tag="VMZ53_16610"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUS30132.1"
                     /translation="MTDGGDTHAAAAMVSLRRIIRYLRLADREIESSAGLSSAQLFVL
                     TTLAEAPAISLSEIAKRALTDQSSVSTVVARLVERGLIARKASTKDRRRAELRLTPAG
                     HRLLTTAPAVPQARLIEAIRAMPVARRNELVRALDALVDAIGADAVKPRMFFQDEQP"
     gene            complement(15085..17619)
                     /locus_tag="VMZ53_16615"
     CDS             complement(15085..17619)
                     /locus_tag="VMZ53_16615"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HUS30133.1"
                     /translation="MDRTLVISATNVLAAGFLVVPTDRKSPAGDPVNGLFAVARAIHR
                     VLQFKVPARAVAVVGPADPKWPALLQAQLPALPALLRALGVHVVEASDEQHLVASYVQ
                     AARDLGDDAIVIGRDKRFAQLVSDHVWWYDSNKDVRYTPEIVHKRFTVGPETVAEWLA
                     LVGDQEALPGVKGIGAKGATSIIETYGSVDAAMATLDTIEGRIGKALRAIRDDVPKEL
                     ARARLDRHRPLPRPLAELTYALPAAATLNAAYEQLGFAELLSVGDVAIRAEVCETAES
                     LRGVLAALHGTVALHALLEDDGALTGIALTTGGGDAWYVRTASAAWPELATWLGSAAP
                     KVGHDLLGVRVALCRMNVDLAGVVADSMCASHLAQPSNWAPHDLGLVAKHVLGRALSE
                     DDQVRGVGRARKAWSALPPEQAAGVAGQRAETALAIWNALAKTIDRERLDEYLALTET
                     CMRMELRGIIVDPAALDHAQEAFAQIEAELTAQIEALAGHSFNINSGQQLGTVLFEEL
                     KLPIVSHTKTGWSTAIEALERIENAHPIVPLVIRWRLLRRLRDFWVIALRKNIGEDGR
                     VHSRSHPARSFSGHLVNTNPDLSRVPGRTAEMQLIRRAFVAPPGCLLMSVDFNQLGLH
                     VLAHLTKDPALVEPLRARKDMHVLTAMAVLDKPADQITDEERQLGKVVNFATFAGQGP
                     SALALQLGVPAAEAKEYIARFDRHYALVRAFQDEQLRLAKERGYIMTIAGRHWPIGGL
                     ESLDNHDRSYAERLARRATHEGSVADVARRALLDADRALRAAGLHATPLVQVVDEVLF
                     EVRESELVQSAKIAADAMRHAFELEVPLVVGAWAGRNWAELEEVPL"
     gene            17853..18395
                     /locus_tag="VMZ53_16620"
     CDS             17853..18395
                     /locus_tag="VMZ53_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30134.1"
                     /translation="MEQPWQQLVRPSVSRADYLAQLVRTYGLIAPFESACKYTPNLPR
                     IVDTRHQSRAGLLVLDLLAVGLSASQIASIPQCMPAASFGDVNEALGWLYVIERATPA
                     HDSVRVHLTAALPDVGHAFSFLEAHDTRAQYRWEMFAGALDRAWDVSELEIISAAQFA
                     FQTVTVWLRCNSTPLARLGG"
     gene            complement(18392..19186)
                     /locus_tag="VMZ53_16625"
     CDS             complement(18392..19186)
                     /locus_tag="VMZ53_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HUS30135.1"
                     /translation="MSGKSNGVLRLRYAARTDVGMRRNHNEDCFGILEDERLVLVADG
                     MGGHAAGEIASGIATTEVSSFYQRTLSGNGEPWPFEPNPQLSKLENRLVCAFKVANLR
                     IFETARGDGGRRGMGTTLAAAAIDNGQICIAHVGDSRVYRIHGEQIERLTRDHSLLEQ
                     FKEINPGMTREEENNFPHKNVITRALGLNDEVEVEVMRMDLEIGDEYLLCSDGLSGHI
                     TDEEMLEIVKESEGDLPLAVAKLVDMANDAGGNDNITVVLVRCTNS"
     gene            complement(19239..19559)
                     /locus_tag="VMZ53_16630"
     CDS             complement(19239..19559)
                     /locus_tag="VMZ53_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30136.1"
                     /translation="MVCPRCAAEMPDGKGGPWCAECEKLYDTWSRRHAADILWQTGIG
                     ALVAMAIGLGLPLLGIEPVIATTGVIAGFSTFLGLRAWGKKRRRKQFLVGALPRAYLP
                     SRTT"
     gene            complement(19579..19995)
                     /locus_tag="VMZ53_16635"
     CDS             complement(19579..19995)
                     /locus_tag="VMZ53_16635"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS30137.1"
                     /translation="MPRKAPLETPLYVRLPTEASDKLERAANALGVRKKDLVANLVST
                     YVDPDSKQGLSALGTLATRKVLDTNQTGPMLGAYSFQSYSQDFPDVMNSKQAGQFLQI
                     EEKLVIELAEKGDLPGKKLGPVWRFSRDALVAWLAG"
     gene            complement(20026..20661)
                     /locus_tag="VMZ53_16640"
     CDS             complement(20026..20661)
                     /locus_tag="VMZ53_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30138.1"
                     /translation="MRTLLVVALVSLAGARAARAESEHFEQIRADGGLGASKAALDDR
                     GGISLIAEVKAMVHDDLAVGGRVEIAMIYGGVVGMDELPLDFTLAASGLVKAELYLPG
                     SQRYLVRPFVGLGAGAFTIGSHQIQSGPNTDGISTSIGRYFGVAPQIGIDIGRVRLAA
                     TYNAILGATLEVHDRVGTAEEIHRVSQNYLTLELSFRFAGARKRAGSSTAY"
     gene            complement(20661..21311)
                     /locus_tag="VMZ53_16645"
     CDS             complement(20661..21311)
                     /locus_tag="VMZ53_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30139.1"
                     /translation="MLRNSLVLVSLVSLTAVAHAQGAAGAPAEPAADPVATPAAADPT
                     LVPVAPTPPAPESQTPQTAKGLRNGFSLSVGQEFGTTMAGQSFSGQLYGVDWRIGARF
                     SEAISVYLHSHISFGTVGPDQGGGGGVTGNIAGAVVAEYMLPMRLFAGAGAGYGVLNN
                     PNGPLVEARAGYYPFKTNASGKARRLNVALDARWYMVGDQIGTVTQLSLSLGYDRF"
     gene            21652..21903
                     /locus_tag="VMZ53_16650"
     CDS             21652..21903
                     /locus_tag="VMZ53_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30140.1"
                     /translation="MNKETRMNNEITTIADAELDGVTGGWAAWLGKAATMLGGLFGGG
                     TNINAQVGNNQRSAQGNTGPVSIGDGSPITTTGQQGGSE"
     gene            21906..22118
                     /locus_tag="VMZ53_16655"
     CDS             21906..22118
                     /locus_tag="VMZ53_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30141.1"
                     /translation="MELTTEELAGVTGAWLNVGFAWGDGNRIAAGTGTRGLNFQFAWG
                     NNNHIETGQGPTLRMDFGSGNTFESK"
     gene            22127..22384
                     /locus_tag="VMZ53_16660"
     CDS             22127..22384
                     /locus_tag="VMZ53_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30142.1"
                     /translation="MNNKDAFTSISLDNLEHVTGGAGKAGGWGSQAWSWIKDTASSLF
                     SGEGTGTNINAQIGNNQRSAQGNTGPVSLGDNSPVTVNGQQ"
     gene            22384..22533
                     /locus_tag="VMZ53_16665"
     CDS             22384..22533
                     /locus_tag="VMZ53_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30143.1"
                     /translation="MSKKPSAFEELKARFFAKLNIAIVTGNNRRVSVGNKGTVTLEDN
                     QPIKE"
     gene            22538..22753
                     /locus_tag="VMZ53_16670"
     CDS             22538..22753
                     /locus_tag="VMZ53_16670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HUS30144.1"
                     /translation="METISNEELENVNGGILPWLVAGGIAAGAAAWAYYGGGVNAGIA
                     IGDNNRQAVGNQAPVALGDNSGVQVNK"
     gene            22898..>23335
                     /locus_tag="VMZ53_16675"
     CDS             22898..>23335
                     /locus_tag="VMZ53_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30145.1"
                     /translation="MRAVIALLIAGCAHSPPVVEKTPCPAPAPAPAPVVAPATAAVPA
                     AWDDAKVIEKSQAFLAAADTYARPSAVEDPAMITFSGGRTYDAAMVKRVATRLKEHHL
                     PPQTRTCDKQRVLRTESTMMYFDRCEITTPAHDDVPQLTYSEYD"
BASE COUNT         4132 a         7716 c         7682 g         3805 t
ORIGIN      
        1 cgctttgtcg cagctctcga tcgcggccgt gaagtcaccc cactgcagct cgcagatgcc
       61 gcgagccgag cgcgcgaacg cgacgacgac gggatcgccg acttctgccg cgagcttctc
      121 ggcggcttgc acgatgcgcc ctgcacgatc gcgcgtcgaa gagccccacg cagcgacgaa
      181 gcatgcctcg atcgctagag cgcgagcggc gcgcgctgga tcgccggact cgagagcgaa
      241 gcgtgtgctg cgcgtttgaa agaccgcgcc ggtgatcgtg tcgaccatcg cgagccccgt
      301 cgagagcgag tagcaggcat cggacttcgc agcgatcgcg gaatcgacgt cgccgcggcg
      361 cggctcgaga ccgcgaaggc gcaagcgcgc gcgctcgacg agcagctgtg cgatcgcctt
      421 ggccggcgat gcctgcgcgg catagccgag ctgcttcgcg acacggccgg cgatctccat
      481 gccctcggcg aggctgccgc tgcgcaagaa gttctcggcc gcgcggcgct cgagatcgag
      541 gcgctcggtt cccgcggcag tctcggcggc atcgcgatac gcgaatgcag cacggctcgc
      601 atgaccgctg ccggcgagcg catcggcata cgcgcgcgac agcgtggact tgctcgttgc
      661 cgagagcgcg agcgcattga cgacttcgca gacatccgca gcacgcgagt aggcgagggc
      721 gtggcgcgcg tttgcgacga gagcgatgac ttccttcgct gcgcgctcgg cgtcgccggc
      781 ccaggcccag tgcaacgcgc gtcgctccgg ctgcaagaac ggcatcgact cgagaccctt
      841 cgcgagccgc acgtggtttg cgcgaagctc gtcgggtgag agcaaggcga gcgtcgcttc
      901 ggcgacttgt gcgtgaaacg gctcgacgtg gtcgctgcgc cgaaggccgg tgacactcac
      961 gaggcgctgc gccagcaagc cgcgtaattc gtcgacgagc acgaacggct cgcgctcgag
     1021 cgcagcaccg agcagatcga tgcgcagcgg tgcggccgag acggcgacga cgcccagcag
     1081 ctcgcggagc ggcggcgcga gatctttgat cgtgcggcgc atcgtgcgat ggagcgcatc
     1141 cgatccttgc gacgtgctcg cgagcagctc gaggaagagc ggatggcctt cggtctcctt
     1201 cgcgatcttg tccgcgcgat cgcggtcgcg aacgagcagc tgcgcgaggg agcgcgcttc
     1261 gttcggctcg agaggtccga gcgccatcat cgacaggtcg agatcgccct cggacacacg
     1321 cccgagccag ttcgtaatgg cttccgtcga ggcctctgtc gtgcgcaagc caaacacgag
     1381 cgcgatcggc ggcgcctccg gcggctcgag cagctgggcg aacagctcga ggctcgcctc
     1441 ggtggcccac tgcacatcgt cgatcacgat gacgacgttc atcttcagcg cgatgcgacg
     1501 aagcagctcg cggagcgcga caatcgcgcg gtgctcgagc tctgcagcgg gaaggctcga
     1561 gctcgtgctc accggcagga cgcgcgcgaa aatcggaaac aagcgcgaga tcgcctcggc
     1621 gttcggtggc agcagctcgg tcgcctcggc gcgcgggatg ctggacagca tgcgcgtcag
     1681 accgtcgagc agcgcctccc acgcgttgag ccgcacatgc tcgcgcacgc gacagcgacc
     1741 gaggcagaca aacagcccgt agtcgcgaca cgcctgcgtg aagcgcccga gcagccgcgt
     1801 cttgccaatg cccgacgcgc cggtgacgac gcgcaccgac ggagccttgc gcgaacgctc
     1861 cgcagcacgc agcagcgctt tgagctcttg ttcgcggccg acaaacgcgc gcgccggacg
     1921 cacagccgca ccgacaaccg ccggctccga cgaccgctgc gctggctcgg ccgcagcgat
     1981 acgccgtgcg acgtcctctg caggcggccg ctttgccgga tcacgcgaca agagctccat
     2041 gcacagcgcg tcgaggtccg gcggcacctc gggaaagagc aggctcggcg cgatcggatc
     2101 gctcgtcgtt ttctcgacga tgacgacgac gttcgggccg acaaacggcg ggcgaccgac
     2161 gagcgcctcg tagagcacgg cacccatcgc atagagatcg gccgccgtcg acacgatgcc
     2221 gctctcggtc tgctccggtg ccatgtacgg gaccgtgccg gcgagctgcg tcgcatcgga
     2281 cagatcgatc gccgcggccg cgagcccgag gtcgagcagg accacgcgac catccggcgc
     2341 gacgcggatg ttcgaaggct tcacatcgcg gtgaacgatg cccgatgcgt gaagcgcagc
     2401 aagaccggcg gcgagctgac cgatcgtctt gcgcaacttc gtctcgtcga agttcgcctc
     2461 gtcggtccac gatttctcgc cggactcgtc cgccgttggc agccgcaagc ctctcggcgc
     2521 ggtcggcgct tccgcgctca cgttcacgcg aggccgtcgc gcgacgtgcg tgatcacatc
     2581 gacgccgttg acgagctcca tcgagaagca ccaccgctgc ccgtgctcga acagctcacc
     2641 gagcttcacg agattctcgt gacgaacgtc ggcagcgacg cgaaactcgt gcttgagctt
     2701 ttcgatggcc tcaccatcgc cctcgtgcaa gatcttgaga gcgacgtgga tcgcgagctc
     2761 ttgatcgcgc gcctcgtaca cttttcccat gccgccctcg ccgagctcgc gaacaatcgc
     2821 gaagcgttgc gggaccgaga cgtcagctcg gacggcgtcc accacgagcg gccatcgtac
     2881 cgcgttatag ttctcgcgtg agccagccgc tcgacacgtc tcagacttcc ggcatccgcc
     2941 acggcagcaa ggtgggccgg ttcgtcgtgg aagggcagct cggcgccggt gggatgggtg
     3001 tggtctacgc tgcgcatgat cgcgagctcg accgccgcgt cgcgctcaag gtgctgaaag
     3061 gcaccgacga cgccgagcag cggacgcgcc tgatgcgcga aggccaggcg atggcgaggg
     3121 tgacgcacga gaacgtcatc accgtccacg aagtcggcat ggaaggcagc atggtcttcc
     3181 tcgcgcagga gttgctcgac ggtggctcgc tccgcgactg gctgctcgcc gacaagaaac
     3241 ggtcgcagtc ggagattctc gcaaagttca tcgcagcggg acgcgggctc gcggccgcac
     3301 atcgcgcggg gctcgtgcat cgcgacttca agcccgacaa cgtgctgctc ggcaaagacg
     3361 gtcgcgtgcg cgtctcggat tttgggctcg cgcggtcgct cgagagcagc gcggcggcac
     3421 tcgccgaaac gcaaaagggc gggggctcga cggacgagaa cctggcgaac ccgatgctga
     3481 cgatgacgcg cacgggcgcc gtgatgggca cgccgctcta tatgtcgccc gagcagcacg
     3541 cgggtgagcc ggccgacgag cgctcggatc agttcagctt ctgcgtcgcg ctctacgaag
     3601 cgctgcacgg cgacacgccg ttcccgggca agaccgccgt cgcgctcgcg gatgccgtga
     3661 tgtcgggacg catgaagccg ccgcccaaga ccgcggacgt gccgtcgcgc attcgcaaga
     3721 tcttgctgcg cggcctctcg acgatccccg gcgagcggta tccgtcgatg gacgcactgc
     3781 tggcagatct cacgcacgat ccggcgcgca agcgcacgcg cttcgtgctc ctcgcggctg
     3841 tcatcatctt gatcgcgggc gccgtcgtcg gcggttacgc gctgtcgtca cacgaatcgc
     3901 gcaaggcgag cgaggttccc gcgcgccgca cggtcgcagt gctcgggttc aagaacacgg
     3961 ccgccgatcc agcgagcgcg tggatctcgc aaggcatgtc gcagctgctc gccgcgcagc
     4021 tcctcgccga cgacgtcaag gtcgtgtccg ccgaagaggc actgcgcgca cgcagcgagc
     4081 ttcatcttcc cgataccgat acgtacagcg cagacacgct gaacaagatc cgcgagcggc
     4141 tcggcgcgga cgcggtgatc ggcggctcgt acttcgtctc gaatggcgag ctgacgctcg
     4201 cgatctcggt ccaagacatc ccgcgcaaga agtcgatccc gatccagctc aagggcacga
     4261 cggccgagct accggcgctt gccgagaaag ccggaggtga gatccgcacg gcgctcggca
     4321 tcagctcggt cgcgatcgtc aagaagagct acgacgtgtt gccgcacgac gcggtggcac
     4381 agcgcgagtt catcgagggc aatgaggcgc tccgcaagtt cgatttccgc aacgcaaaga
     4441 tgcacctcct cgccgcggtc aaggcggaac cggacttcgc gccgagtcac ctcgcgctcg
     4501 cgaaggcgta cagcggccta ttcgacgccg atgccgcgcg agcatctgcg aagcaggcgc
     4561 tagccactgc gagctccgtc ggcggtgatc aacagctcgt cgtgcggcgc gatgcgtaca
     4621 agctcctcga tcagatcgtc gaggcgcgcg agcaagcgaa ccagttgttc gtgctccatc
     4681 gcgacaatct cgaatacggc ctcgcgctcg ccgagctgca agagcccgat gatgccgtgc
     4741 gcacgcttgc aacgctccgc aagctcgccg cgcccgccgg aacggatccg cgtatcgata
     4801 tcgccgaggg tcgtgccgag ctggagcggc aggcagcgcc acgcgcgctc gagctcgcac
     4861 gtcacgcgac gcaggaagcg acggcgcgca acgcggcagg cgtcgcagcg gaggcccgcc
     4921 agctcgaggg cgaagtgctg gtcgcgaccg gcgagctggc gaccgcgcaa gatacgttcg
     4981 aggatgcgcg caagcggttc gaagcggcga acgatcaaat tcacaagctg cagacgctcg
     5041 agtcgctcgc ggatctatcg ctcgagcgcg gtcagctcga cgacgcgctc aagagctacg
     5101 acgccctcgc atcgctgcgc gagcaggcag ggcaaaccgg ggtcgctgca cgtgcgaccg
     5161 cgcgggctgc ctatgcactc gcgctcgccg gtaagaacgc tgacgccgag aagcgcctcg
     5221 cgcgtgcgca gaagatggcc gcagacgatc cggtcgcgat cccgacgatc gacctcgtcg
     5281 cgggagttct cggctgggcg aagggcgatg gcccgaccgc gctcggtcgc ctcgacaagt
     5341 gcaacgcgcg atttgcgagc gtcgctccgg gatcgtcggt gctgtgcctg ctattgcgcg
     5401 gcgaaatcca ggcggagctc ggcgacgccg cggctgcgaa gaagacgttc gacgacgcaa
     5461 aggccgtcgc cgacaaagcg ggcaacgtgt atcgcagcca gcaagccgag cttcgcctcg
     5521 cgcagctcga gctcgacgag gagagcccgg cggatgaaga agccaagatc gcggatcgca
     5581 tcgtcgagcg agcaggggcc atccgcatcg ccgcggcgac gcacggtgcg acgagcctcg
     5641 aggcgcgggc cggcatcatg ctcgcgcgcg cgcacctcgc gcagggcaac tcgcaggacg
     5701 cgctcgacgt gatcgcgaag gttcaaaagc aggaggacat tcgcatcgag gtcgatcgtc
     5761 tgatcaccga tgcgctgtcg aaggacgcgg cgggcgacgc gacctcgcgc gataccctcg
     5821 acgaagtgaa gaagatcgcg gacaagcagg gctgcgtgac gctgcagctc gaagcgaggc
     5881 tcgcactcgc cgaagtgctc gcaacatcga aggagcctgg tgagaccgaa aagggcaggg
     5941 ccgagctcgc gacgatccaa caagaagccg tcgccaagaa cctcggtcgg atcgcgaagc
     6001 gcgccgagat cctctcgcgt ccgtgagcta gactcgtccc gccatggccg attgccagct
     6061 gtgcgggtcg caccacaaag aggacatctg cccgcacgcg cgcacgggac aggtcatcaa
     6121 caactacgtt gtcgggccgc tactcggcgt cggcggcatt gcagcggtct actcggcgcg
     6181 ccatctaaag ctcggccgcg agatcgcgat gaagatcttg caccagcggt gggcgaagga
     6241 tcacgagctg tcgtcgcgat tcgtgcgcga aggaaaagcg accgcggcgg tcggtcatcc
     6301 cgcgttcgtc accgtcttcg acgccggcac gaccgaggac ggctgcgcgt acatcgagat
     6361 ggacaagctc gtcggtcgcg acctctacgc ggtgcgcacg caagacggtc cactcgcggt
     6421 cgatcgtgtc atctcgattg cgatccaggt cctcgacggt ctctcggtac ttcacagccg
     6481 cggcatgatc catcgcgacc tcaagagctc gaacatctac ctcacgccga gcgagatggg
     6541 cgagcgcgtc gtgctcctcg atctcggctt cgcgaagctc gatgacgggc tcgcccttac
     6601 gtcgaacgat cagatgctcg gcacgccgtt ctacatctcg cccgagcagt atctcgatcc
     6661 cacgcaggtc gacgcgcgcg ccgatctgtt ctcgctcggc atcgtgatgt gggaagtcct
     6721 cgtcggtgat ttcccgtacg agtgggagaa caagcgcgac ctcctcgcga aggtcatgca
     6781 cggcaagctc gagcggcatc ctgcggtgcg ccgtcccgac gtgcccgcat ggctcgacga
     6841 gatcatcgcg accgcgctcg cccacaagcg cgaggatcgc ttcgagtcgg cggcggtgat
     6901 gaaggctgcg ctcgacggca aagagctccc cgagaagcct ggcttcttga agagaatgtt
     6961 cggccgctag ccggtcatcg caacgagcgt ctgcacggtt cgccaattgc gcgccgttgc
     7021 ttcatcgatc ggtggcttgc gcatgagagt gccggcgagc ttcgagcgcc cgagcccgtt
     7081 cggcaagtag acgtagagct ctcggtcgac gatcgcgaat ttctctggct cgaacgcttt
     7141 cgcgtcgacc ttcgcgagcg catctcgcgc tggtttcttc gccatgaaga acacgtggag
     7201 ctgctggtcg gtcgccttct tgaacgggtt cttcgcgacg atcttcgcga gctcggcctt
     7261 cgtgcgcacg acgaccttca cgtcgaactt ggcatccttc gcgatcgccg cctcgacgag
     7321 cgcgacgagc ttcgcttcag acttctcggc gtgggcgaac acgacgttgc cgctctggat
     7381 atacgtcgcg acgttcgtgc agccgactcg cgcaaacgtg gcgcggagcg cttgcatctt
     7441 caccgggcgc gacccgccga cgtttattcc ccgcaagagc gctacgtaga tcggcacgct
     7501 gccgacctta ccgggcattg ccagaccttg cattggccgg accgcgccgc gatgcgcacg
     7561 ttaatcgtgt gctcgcattt gtccctctga tctcggccgc cgccggtggc ctcgcgtacc
     7621 tcgcctgcta cggcggcatc gccggctcgc atggtccgcg tgacaagcgg cgcatcgcgc
     7681 tgacgttcga tgacggtccc gatccactgc ggactccagc actcctcgac gcgcttgccg
     7741 cgctcgatgt tccggcgacg ttcttcctcg tcggtcgcgc ggtcgacgcg caccccgagc
     7801 tcgcagctcg catcgcacgc gaaggccacg agatcggcaa tcacacgtac agccatcgct
     7861 acttgccact cgctcgctcg cgcagcgtcg ccgccgagct tgctgcaacg gacgctgcga
     7921 tcgcacgcgc gaccggaatc gtgcccgtgc tcgcacgacc tccgtacggc ggccgttcgc
     7981 atcggaacgt gcgcgcgttc gcgaggagcg agaaacgcct cgtcacgtgg gacgtcaaca
     8041 gcttcgactg gaagggcaag ccggccgatg ttgtcgttcg ccgcgtcctc gagcgcacgc
     8101 gtccgggcag catcgtcctc atgcacgagg cgcgcgaggg cggcgaggtg acgatcgagg
     8161 ccgtgcgaca gctcgtgccg gagcttcgcc agcaggggtt cgagctgacg acggtgagcc
     8221 gcacgctcgg catgtgagtg atacgatgcc gtcggcatgg caggtggtga tgacgacggc
     8281 gagcaggcaa ctcacgtaga gggccgattc tcgcgtcgca tgacgacggt gaagccaccg
     8341 ccggcggcac gtctcgaaga tcgcggtgag atcgcgcgag gtggccaggg cgtcatccgc
     8401 aagatgttcg atcacaacct gcatcgctat gtcgcgcaga aggtgatgga ccccgagctg
     8461 ctgcaccacc ccgaccagcg tgggcggttc ctcgacgagg cgcgcatcac gggtcagctc
     8521 gaccatccga acatcgtccc gatttacgac gtcgtcgagg aagagcattc gaccgagttc
     8581 gtcatgaagc tcgtcgaggg cgagacgctc tcgaagcggc tgaagcgtcg caagagcgca
     8641 gacgaccttc ctcgttttct cgagatcttc ctcaaggtct gcgacgcgat cgcgttcgcg
     8701 cacagccgcg gcgtcatcca ccgcgatctc aagcccgcga acatcatgat cggcgagttc
     8761 ggcgaggtct acgtgatgga ctggggctgc gcacatattc tcccgcgcaa gggccagctc
     8821 tccgagaaca cgccacagcg catgatcgtc gacgagcctg gcacggtcat cggtacgagc
     8881 gcgtatatgt cgccggagca ggcgcacggg cgcacggacg agatcgacga acgcaccgac
     8941 gtgttcggcc tcggcgcggt gctctacgag atcctgacgg gcagcccgcc gtatcccggt
     9001 tcgaatgacg accaacaaag cgcgctcgcc cgcgcaggga aggtccgccc acccgaggaa
     9061 gtcgagaagc tgcgcacgcc gccgcctggc gtctcggcga tcgcgatgcg cgctctctcc
     9121 gcgaaacgag aagatcgcca gcaaacggtg gtcgcgctgc gcgacgagat cgagcgcttc
     9181 ctccatcgtg gcgcattctt cacgccgcgc ccgtttcccg ccggcaccct gatcgttcgt
     9241 gaaggcgaca tcgccgacga ggcgtacatc atcacgtcgg gccactgcga agcattccgc
     9301 gaggagcgcg gccgcaagct ctcgctgcgc acgctcggcc ccggcgaggt gttcggcgag
     9361 ggcgcattgt tcgcacgcga gccccgcaac gcgagcatcg ttgccgtcga tgacgtgacc
     9421 gcgatcgtgc tgacgcaaca ggtgctcgat cgcgagctcg gtacggactc gtggctcggc
     9481 acgttcattc gcgcactcac tgccagatat cgcgacctcg aagcccgcga catgctcacg
     9541 cgacgcgtga tcgagaacgg ccgcatcgcc gccgagatca tcaatcacat cagccgcgcg
     9601 ggccgctggg ttcgcccggg tgtgctcggc acgagctggt cgcgcgtgtg gtctgcgctg
     9661 cagccggtcc tccacatcac cgaggatcag gcactctcga tcgtggccgg cacgcaagac
     9721 ctcgactacg acgcaccgcg cgacgagatc acgctcgctc ttctttcgat ggcgtagcga
     9781 tgtcgaagca tcccgtcacg atggcgatcc gcgcgctgcg tactggcaag gtcgacttcg
     9841 tcccgcacct ctacgcgtgg caaccgcacg gaggcacccg cgcatcggcc gaagcattcg
     9901 gcgtcgacga gcacatcgtg atcaagacgc tgatcttcga ggacgacgcg aagaagccgc
     9961 tctgcatctt gatgcacgga gaccgcgagg tctcggcgaa gcagctcgcg cgcgtcatcg
    10021 gtgcgaagac cgtctcgccg tgcacgccgg aaattgccga tcgccattcc ggctatcagg
    10081 tcggcggaac gtcgccgttt ggcctgcgca agcagatgcc ggtctatatg gaacgcacga
    10141 tcgccgagct gcctcgcatc tacatcaacg gaggcgcgcg gggctttctc gtcgagatcg
    10201 atccgaagga cgcgattcgc gtgctgtcgc cgacgctcgt cgacgtcgct acgacctgag
    10261 ggatcgccgt acttcgatgg cgcgcacgtt gctcttctgc agagcatgcg gtccaagatt
    10321 tctgcactgc tgttcgtggt gttcgcggcg acgggcgtaa cggcccgtgc ggacgtcgtc
    10381 tgccgagacg ggacgagctc ggtcgcgtcc ggccgcggcg cttgctcgca tcacggtggt
    10441 gtcgcgaaga aacgcgccga gcgcacgcgc gctccgaaac gtgccgacga cgtcgtgctc
    10501 gagcctcgcg agcaaagccg caccgatcgc attcgccgcc gtggtgaacg ttcgcacccg
    10561 atcgagaagc gcgagtctgg atcgccaacc gccgtctgtc gcgacggcaa gctgtcgtac
    10621 gcactgcatc atcgcggcgc atgctcgggc cacgatggcg tcgagcggtg gttggactaa
    10681 ttcgggatca tctcgacgcc gatgaacggc gcgatcggta gaccgcgcga ctccttgatc
    10741 agcaacgagt cgacgtcgaa cgtgcggccc tcgacgttgc ggcggttcgt gatgttctgg
    10801 atatcggcgt agaagttcac gtcgccccag cactgcggcc acttgcgatc ggcgcgtacg
    10861 tcgagctgga agaacaccgg caagttgccc gtcagcggcg cgggcagcgg cgtgcccgat
    10921 acaaggtgaa tgcgcgcgcc tacgcgccac ttgtcgaggg ggatcgaagc ggcgacgttg
    10981 aggttgtggc gctggtccag ctcgaacggt cgccagccgg cgcgaatctc gggatcgtcg
    11041 gtgcgctccg attcggacag cgtgtacgag agcatcccga accaggtctt cgacgagtgc
    11101 ttgatcatca gctcgagacc tttgctgcgc gcgcggccga tgttgctgcg aacgaatgca
    11161 aggccgagct gcttctcgag caggagctcg aatgtcgggc cgagaccatc gaggttcgcg
    11221 aagttcttct ggtcgagcat cgtgcgaacg ccgaggtcgc gtccgatgtt gtagaagccg
    11281 gtgaacgagc cggtccagcc ttcgggcagc tcacgctcga tgccgatcga tgctgcgtcg
    11341 acgtacgagc tcgtcagctc gggattgccg ccattgggat cgacgtcacc tggcgtcggc
    11401 ggttggtgat agcgcccgat cgtctcgcgg aacgtcagct ccttcgtcag cttctcttga
    11461 atcgcgagcc gcggatcgat caccgcttcg cccgtcagac cgtagtgctc gagacgcaaa
    11521 ccgggtcgga gcgagatgcg ctctgcgagc gtgaagcgaa agtcgctcca gatcgcggcg
    11581 tccatccagt tcaccgtcgt gtcgccgttg acgagcgaga tcagatcgcc gcggtgaccg
    11641 aacccggctt ggtaccgcga gagatgacca gcttggatat cgaggccagt tcgcagcgtg
    11701 cccgcgggat agtcgtgcga gagcgtcgcg cgcgttccgg ccgtgtacac ggggcgtgtg
    11761 aacttctctt gcacgccatt cgtgacgctg ccaaacgtca gctggttcgt gccgagccag
    11821 ggcacgatct cgatcgtctg gtgctccagc tcccgcttgt acggcatcgc gacgcgaacg
    11881 aagaacgcgc gcatcgacga ttcgtcttcg aactgcgggt ccttgcccgg ctgcgacgcg
    11941 tagatgtcgt cgagcgagag gaacgccatc ggcgcgatgt ggccgccttc cttcttcgga
    12001 tcgccgaacg acatgcggac ctgcgcatcc cagtagctcg gcagtggaac gtcgtcgggt
    12061 gcaaccggac cggcgacgat gtcgaagtac gagcgacgaa gaccgatcgc aatcgagccg
    12121 ctcctgaacg ggccctcgac gatcgccgat gcatcgagca gtccgatcga accgcctttg
    12181 cgccaccgat cgcgacgtgg ctcgcgcgtc gtcagcgaga cgatgccgcc ttgtgcgcgt
    12241 ccatacgacg cttcgagacc gccgttgctg acggagagac ccgacaacat gttgctcgga
    12301 tagaacgagg tgatgccgcc gaagtgaaac gcgagcggta cctcgatgcc gtcgagatag
    12361 acggcgttgt cgcgaggtga cgtgccgcgc aggacgatgc cgccgaacga gaatggcaag
    12421 cgcgagacgc cgggcaacgc ttgtgctgca cgcagcacgt cgtttgcggt gccgggaagg
    12481 ctgcgcagat catcggaggt cagctcgtat ttctttgccg cgggttgcac tggaagcctg
    12541 ccctcgacgt cgatcacttc ggcgccggac gcgctctcga gctcgatgcg caacgacgtg
    12601 gacgtgagct tcacgacgcg cattgcgtag cccggcgccg tcacgagtac ctcgacctcg
    12661 tcgggcgcga gcatgatcgt gaaggtgccc gtgacgctgg tcgtctcgga tacgttcgcg
    12721 ccgacgacga gcgcgccctc gacaggctcg ccggtcgcct tgtcgatcac gacgccggag
    12781 acgtgttgat agggtgccgc ttcgaccacc gaaacggtga tcgcgagcaa cagccacacc
    12841 cgaagcacgg atcgaggtta caggccctcg gcgtaacgag gttgcaacgt ggcggcaaga
    12901 tccaacgaga gcgtggctcc ccagccgtcg ggagtgcgga tggcggcgag tgtggctgca
    12961 ggctggagcc accggctctt caacatgccc gtgatgccgg cgctccagcg cagcccgcca
    13021 tcctcgacga acggatcgta cgagagcgcg gtctcgaacg agacggaacc aagatcacac
    13081 tgctgcatca cgcccgtgcc gatgcgcggg atcatgtgga cgatgcgccg gtggtcctcc
    13141 gtcgcgtatt cgccggcgac atcgaggccg acgtcgagcc gcatgatgtg cttggcgacc
    13201 gacgtccacg gcgcatcctt gatccagatc tgcgagtgac tcatacgtac gcccatcgtc
    13261 cgaggcagct cgaggactgc atctccgacg cgtgcgctga cgggaagcgg cgcctgttcg
    13321 cgcgacgcgc tcgggaggag cacgccgagc cgatagatca tctggtcgcg cccacgttcg
    13381 ctgccgccga agatgccgac ctcggacgtg ccgatcgata cgcgggaatc gtcacttgga
    13441 atcgcctgcc cggcggccgc tacgcccggg tcctccgaga ccgagatcgc gaggggcagc
    13501 gtgccgtacg caccgatgtg gcagtcgcat tgggtcgaca tctgcgcatg gagcgtcgac
    13561 gtcacggccg ggccgccatt gatctcgatg gtgtcgctga tccgaggcgg ggatagcgtt
    13621 tggcggtcga gcaggccgaa cggaagccag aggggagcga ggaacggcat acccgttaca
    13681 cgcagcgggc ctgcaggatg agacgaatag tttctgccat cgaaccatgg gcgcccatgg
    13741 atctctgagc ctcctcggac taggctggtc gcgggagatc accgatgaac atcgacgaca
    13801 tcaagcctgc caactgctac gcccgcgccc gccgtctgct cggcgaagtg tcactggtcc
    13861 gagacgagct cggccgcagc gaggatacgc gtcaggtccc agagatcacc ggcgcgcaac
    13921 cgcgcgagtg ctacttcgaa gcgatcgctg cgtggcgcaa agccgaaagg cttgccgacg
    13981 agctcggcgt gcacaagtcc cgcgctctcc cgcggacgcc gacgctgcgc gacatccgcc
    14041 ccggccacgt ctaccaagtg ctcgagggag tcctgcatac gctcggcgaa gtgaagtcgc
    14101 gcctcgatat tgcggaatct gtcgtcgagc caggcgtcga accgtcccgg cagccgagcg
    14161 acgtactcgc gacgatcatt cgcatcaacc gcgagctgtc gcgttgcctc gagcgtccgt
    14221 tcacgccgac cgacgtctac gagacggtcg cgctggcgtc ggcgtacgcg tcgcaactcg
    14281 gtgcgacggc agagctcgcg ccgttcgagc gcaagcgcaa gccgcagcat tgctacgaga
    14341 tgctggcgtc gtgcctcacg gcggctgcga gccacgtgac gcagaaaggc gagcaagcgc
    14401 tcgtcaagcg cggcatgcct gtcgatgtcc tgcccggtga cgtgtacgac ctcgcgatcc
    14461 tcgtgctcgg cgagttggcg ttcctgcact cgctgacccc gaatgccgcc acggtccagc
    14521 cgttcacccc cgtcggtcgc ggtcatcgca tcccgtcgga ctgctaccaa ctggcgagga
    14581 ccctggagaa gcagttaacg tccctgaagt gaccgatgga ggcgatacgc atgctgcagc
    14641 ggcgatggtg tcgctgcgta ggatcatccg gtacctgcgt cttgccgatc gcgagatcga
    14701 atcgagcgcg gggctatcat cggcgcagct gttcgttctg acgacgctcg ccgaagcgcc
    14761 ggcgatttcg ctgtccgaga tcgcgaagcg cgcgctgacg gatcagagct cggtgtcgac
    14821 ggttgtcgct cggctcgtcg agcgtggcct gatcgcgcgc aaggcttcta cgaaggatcg
    14881 gcgccgtgcc gagctgaggc tgacgcccgc gggacatcgc ctgctgacga ccgcgccggc
    14941 ggttccacaa gcgcgcttga tcgaggcgat ccgcgcgatg cccgtagcac gccgcaacga
    15001 gctggttcgc gcactcgatg cgctcgtcga tgccatcggt gccgacgcgg tgaagccgcg
    15061 gatgttcttc caggacgagc agccctaaag cggaacttct tcgagctcgg cccagttgcg
    15121 gccggcccac gcaccgacga cgagcggcac ctcgagctcg aacgcgtggc gcatcgcatc
    15181 ggctgcgatc ttcgcggact gcacgagctc gctctcgcgc acttcgaaca acacttcgtc
    15241 gacgacctgc acgagaggcg tcgcgtgaag tcctgcggca cgcagtgcac ggtcggcgtc
    15301 gagcagagcg cggcgtgcga catcggcgac cgagccttcg tgcgtggcgc gacgagcgag
    15361 ccgctcggcg tagctgcgat cgtgattgtc gagcgactcg aggccgccga tcggccagtg
    15421 ccgccccgcg atcgtcatga tgtagccgcg ctccttcgcg agccgcagct gctcgtcttg
    15481 gaacgcacgc acgagtgcgt agtgccgatc gaaacgcgcg atgtattcct tcgcttcggc
    15541 ggcgggcacg ccgagctgga gcgcgagtgc gctcggtcct tgcccggcga acgttgcgaa
    15601 gttgaccacc ttgccgagct ggcgttcctc gtcggtgatc tggtccgccg gcttgtcgag
    15661 cacggccatc gcggtgagga cgtgcatgtc cttgcgcgcg cgcagcggct cgacgagtgc
    15721 cggatccttc gtgagatgcg cgagcacgtg gaggccgagc tgattgaagt cgaccgacat
    15781 cagtagacag ccaggcggtg cgacaaacgc gcggcggatc agctgcatct cggcggtgcg
    15841 cccgggaacg cgtgacagat cgggattggt attgacgagg tggccggaga acgaccgcgc
    15901 gggatgagac cgcgagtgca cgcgtccgtc ctcgccgatg ttcttgcgca gcgcgatcac
    15961 ccagaagtcg cgcaagcgac gaagcaggcg ccagcgaatc acgagcggca cgatcggatg
    16021 tgcgttctcg atgcgctcga gcgcttcgat ggccgtcgac catcccgtct tcgtgtggct
    16081 cacgatcggc agcttcagct cttcgaacag aacggtgccg agctgctggc ccgagttgat
    16141 gttgaacgag tgtcctgcga gcgcttcgat ctgtgcggtc agctccgctt cgatctgcgc
    16201 gaacgcttcc tgcgcgtgat cgagtgcagc cggatcgacg atgatgccgc gcagctccat
    16261 gcgcatgcag gtctcggtga gcgcgagata ctcgtcgagg cgttcgcgat cgatcgtctt
    16321 tgccagcgcg ttccagatcg cgagtgcagt ctcggcgcgt tgaccggcga cgcccgctgc
    16381 ttgctccggc ggcagcgcac tccacgcctt gcgcgcacgc ccgacgccgc gcacctgatc
    16441 gtcttccgac agagcgcgac cgagcacgtg ctttgcgaca agcccgagat catgcggcgc
    16501 ccagttgctc ggctgcgcta gatgcgaagc gcacatcgaa tcggcgacga cacccgcgag
    16561 atccacattc atgcggcaca gcgcgacgcg cacgcccagc aagtcgtggc ctaccttcgg
    16621 cgctgcgctg ccgagccacg tcgctagctc cggccacgct gcgctcgcgg tgcgcacgta
    16681 ccacgcatca ccgccaccgg tcgtcagcgc gatgccggtg agcgcgccgt catcttcgag
    16741 gagcgcgtgc agcgcgacgg ttccgtgcag cgcggcaagc acgccgcgca acgactcggc
    16801 ggtctcgcac acctcggcgc ggatcgcgac atcgccgacg gacaacagtt ccgcaaagcc
    16861 gagctgctcg tatgcggcgt tgagcgtcgc tgcagcggga agcgcgtagg tcagctcggc
    16921 gagaggtcgc ggcaaggggc gatgccgatc gaggcgtgcg cgcgcgagct ccttcggcac
    16981 gtcgtcgcgg atggcgcgca gcgcttttcc gatgcgacct tcgatcgtat cgagcgtcgc
    17041 catcgcggcg tcgacggatc catacgtctc gatgatcgac gtcgcgccct ttgcgccgat
    17101 gcccttcaca ccgggcagcg cttcttgatc accgacgagc gccagccatt cggcgaccgt
    17161 ctccgggccg accgtgaacc gcttgtggac gatctccggc gtgtagcgaa cgtccttgtt
    17221 cgagtcgtac caccacacgt gatcgctcac gagctgcgcg aatcgcttgt cccgcccgat
    17281 cacgatcgcg tcgtcgccga ggtcgcgcgc ggcctgaaca taggacgcga cgaggtgctg
    17341 ctcgtcagac gcctcgacga cgtgaacgcc gagcgcacgc agcagcgcgg ggagcgccgg
    17401 gagctgcgct tggagcaggg ctggccactt cggatccgcc ggaccgacca ccgcgacggc
    17461 gcgcgcgggc accttgaatt gaaggacgcg atggatcgct cgcgccacgg cgaacagacc
    17521 gttgaccgga tcgccggccg gcgatttgcg atcggtcggc acgaccagga acccagccgc
    17581 cagcacgttg gtggccgaga tgacgagcgt ccgatccacg aggacgcgag actaccgccg
    17641 gccgctcccg catggccact ccagacgccg tggcaaacca gagcgtgcgt cttcgctcgc
    17701 cgcggatacg cggcacagtc attccgtgaa agtcatgcag gatcaggggg gcgcggctgc
    17761 gaacgaaaat tgctattggc gctgttgctg tgttcgaggt gagcagcctg ttgaccaggc
    17821 tccaggcggc gacgaggcaa tggcatcccg aggtggaaca gccgtggcag caactcgtgc
    17881 ggccatcggt ctcgcgcgcc gattacctcg cccagctcgt ccgcacctat gggctcatcg
    17941 ccccgttcga gagtgcctgc aagtacacgc caaacctgcc tcggatcgtc gacacccggc
    18001 accagagccg ggcagggctc ctcgtgctcg accttctcgc ggtcggactg tcggcgtcgc
    18061 agatcgcgag catcccgcaa tgcatgcccg ccgcgagctt tggtgacgtc aacgaggcgc
    18121 tcggctggct ctacgtgatc gagcgtgcca cgccggcaca cgattcggtg cgcgtgcacc
    18181 tgacggccgc gctccccgac gtcggtcacg cgttcagctt tctcgaggcc cacgacacgc
    18241 gagcgcaata tcgttgggag atgttcgccg gtgcgctcga tcgcgcatgg gacgtctccg
    18301 agctcgagat catatcggcc gcgcagtttg cgttccagac ggtgacagtc tggctgcgct
    18361 gcaactcgac gccgctcgct cgactcggtg gctagctgtt cgtacagcga acgaggacga
    18421 cggtgatgtt gtcgttgccg ccggcgtcgt tcgccatgtc gacgagcttc gcgacagcga
    18481 gcggaagatc gccttccgac tccttcacga tctcgagcat ctcttcgtcc gtgatgtgac
    18541 cggagagacc gtcactacag agcaggtact cgtcgccgat ctcgaggtcc atgcgcatca
    18601 cttcgacctc gacttcgtcg ttcagcccga gcgcgcgcgt gatgacgttc ttgtgcggga
    18661 agttgttctc ttcctcgcgc gtcatgcctg gattgatctc tttgaactgc tcgagcagcg
    18721 agtgatcgcg cgtgaggcgt tcgatctgct cgccgtggat gcgatacacg cgcgaatcac
    18781 cgacatgcgc gatgcagatc tggccgttgt cgattgcagc cgcggcgagc gtcgtgccca
    18841 tgccgcgcct gccgccgtca ccgcgtgcgg tctcgaagat gcggaggttg gcgaccttga
    18901 acgcacacac gaggcggttc tcgagcttgc tcagctgggg gttcggctcg aacggccacg
    18961 gctcgccgtt gcccgaaagc gtgcgctggt agaacgagct gacctcggtg gtcgcgattc
    19021 ccgatgcgat ttcgccggct gcgtgcccgc ccatgccgtc ggcgacgagg acgaggcgtt
    19081 cgtcttcgag gatgccgaag caatcctcgt tgtgattgcg ccgcatgccg acgtcggtcc
    19141 gtgccgcata gcgcagccgt aggaccccgt tcgacttgcc ggacatgctg aaaggttact
    19201 tcagccgacg cctcgcacgg ggacggatcg tcacttaact aagtggtccg actcggcagg
    19261 tatgcacgcg gcagggcgcc cacgagaaac tgttttctgc ggcgcttctt gccccacgcg
    19321 cgcagcccga gaaacgtcga gaagcctgcg atgacgcccg tcgttgcgat cactggctcg
    19381 atcccgagta gcggcaagcc aagtccgatc gccatcgcga cgagcgcgcc gatgccggtc
    19441 tgccacagga tgtcggccgc gtggcggcgg ctccacgtgt cgtacagctt ctcgcactcg
    19501 gcgcaccacg gcccgccttt gccatccggc atctcggcag cgcagcgcgg gcacaccatc
    19561 agcgaaggat agcgctcgct atccggcgag ccacgcgaca agcgcgtcgc gcgagaatcg
    19621 ccacaccggt ccgagcttct tgccgggcaa gtcgcccttc tcggcgagct cgatgacgag
    19681 cttttcttcg atctgcagaa attgcccggc ctgcttcgag ttcatcacgt cgggaaaatc
    19741 ctgcgagtag gactgaaacg agtaggcgcc gagcatcggg cccgtctgat tcgtgtcgag
    19801 caccttgcgc gttgcgagcg tgccgagcgc gctgaggccc tgcttgctat ccggatcgac
    19861 gtacgtcgac acgaggtttg cgacgagatc cttcttgcga acgccgagcg catttgcggc
    19921 ccgctcgagc ttgtcgctcg cttcggtcgg caaacggaca tacagcgggg tctccagcgg
    19981 agcctttcgg ggcatatcca gaagatatct ggaaatatcc cgatgtcaat acgccgtcga
    20041 ggaacccgcg cgtttgcggg cacctgcgaa ccggaagctc agctcgagcg tcaggtagtt
    20101 ctgcgatacg cgatggatct cttcggcggt tcctacgcgg tcgtggacct cgagcgtcgc
    20161 gccgaggatc gcgttgtacg tcgctgcgag ccgaacccgg ccgatatcga tgccgatctg
    20221 cggcgccaca ccgaagtagc gtccgatcga ggtcgagatt ccgtccgtgt tcggccccga
    20281 ttggatctgg tgcgagccga tcgtgaatgc gcccgcgccc aagccgacga acggccgcac
    20341 gagatatcgc tgcgagcccg gcagatagag ctccgccttc acgagccccg acgccgcgag
    20401 cgtgaagtcg agcggaagct cgtccatgcc gacaacgccg ccgtagatca ttgcgatctc
    20461 gacgcgccca ccgaccgcga ggtcgtcatg caccatcgcc tttacttctg cgatgaggct
    20521 gatgccgccg cgatcgtcga gtgccgcttt cgaggcgccg agcccgccgt ccgcacggat
    20581 ctgctcgaag tgctccgact cggcgcgcgc cgctctcgcc cctgcgagcg agacgagagc
    20641 gactacgagg agcgtcctca ttagaacctg tcgtaaccga gtgacagcga gagctgcgtc
    20701 accgtgccga tctgatcgcc gaccatgtac cagcgcgcat cgagcgcgac gttgaggcga
    20761 cgcgctttgc ccgacgcgtt cgtcttgaac gggtagtaac ccgcacgcgc ttcgacgagc
    20821 ggaccgttcg ggttgttgag cacgccgtaa cccgcgccgg cgcccgcgaa caagcgcatc
    20881 ggcagcatgt actcggcgac gaccgcgccg gcgatgtttc cggtcacgcc accgccaccg
    20941 ccctgatcag gaccgacggt accgaacgag atgtgcgagt gcagatagac cgagatcgct
    21001 tcgctgaagc gagcaccgat gcgccagtcg acaccgtaga gctggccgga gaacgactgt
    21061 cccgccatcg tcgtgccgaa ctcctggcca acggagagcg agaagccgtt gcgcaagccc
    21121 ttcgcggtct gcggagtttg cgactccggt gcaggcggcg taggtgcaac gggcaccagt
    21181 gtgggatccg cggcagcagg cgttgcgaca ggatccgcag cgggttcggc aggagcgccg
    21241 gctgcgcctt gcgcgtgtgc gacagcggtc agcgagacaa gcgacaccaa gacaagcgag
    21301 ttacgcaaca tagagttctc cttgagcaat aggagaccca cgccgtcaga acacctgacg
    21361 gtccgagccc cggacgctcg ttaaggcaag ctcgatgcca cgggcttgtc ggctcgcaac
    21421 tccgtaacgc ccgtcgcgcg gtcaactggc gttctcacca cagtgctggc gggacgtcat
    21481 gcgcgatcag cgcacatccg gatcggccgt ttgcgaccgg gtgcagaccg tctcccggcg
    21541 gacgcgggtt gtctcgcgaa ggtgacgagc ccgaggcagc agtctcgcgc aattccgcga
    21601 aaccccgcgc ttctgcgtcc ggcacggcag ctgctctagc cgtcatccac gatgaacaag
    21661 gagacgcgca tgaacaacga gatcacgacg attgcggacg cagagctcga cggcgttacc
    21721 ggcggctggg ccgcgtggct tggcaaagcg gcaacgatgc tcggcggcct gttcggcggc
    21781 ggcacgaaca tcaacgcgca ggtcggcaat aaccagcgca gcgcgcaggg caacacgggg
    21841 ccggtctcga tcggcgatgg cagcccgatc acgacgacgg gtcagcaggg tgggagcgag
    21901 taaacgtgga gctcacgact gaagagctcg ccggcgtaac cggcgcgtgg ctcaacgtcg
    21961 ggttcgcgtg gggcgacggc aaccgcatcg ctgcgggcac cggaacgcgc ggcctcaact
    22021 tccagttcgc gtggggcaac aacaatcaca tcgagacggg ccaaggcccg acgcttcgga
    22081 tggacttcgg cagcggcaac accttcgaga gcaagtaagg atcaccatga acaacaaaga
    22141 cgcattcact tcgatctcgc tcgacaacct cgagcacgtg accggcggcg cgggcaaggc
    22201 aggcggctgg ggctcgcaag cgtggagctg gatcaaggac accgccagca gcctgttcag
    22261 cggtgaaggc accggcacga acatcaacgc gcagatcggc aacaaccagc gcagcgcgca
    22321 aggcaacacg ggcccggtct cgctcggcga caacagccct gtcacggtca acggtcagca
    22381 gtaatgtcga agaagccttc cgccttcgaa gagctcaagg cgcgcttctt cgccaagctc
    22441 aacattgcga tcgtcacggg caacaaccgt cgcgtctcgg tcggcaacaa gggcacggtc
    22501 acgctcgaag acaaccagcc gatcaaggag tgatccgatg gagacgatct cgaacgaaga
    22561 gctcgagaat gtgaacggcg gcatcctccc gtggctcgtc gcgggtggca tcgcggccgg
    22621 cgccgcagcg tgggcgtact acggcggtgg cgtcaacgcg ggcatcgcga tcggtgacaa
    22681 caaccgccaa gccgtcggca accaagctcc agtcgcgctc ggcgataaca gcggcgtgca
    22741 ggtcaataag tagaaaattg gactcgaatc tcgtttcgag atcatctggc gaagatgacg
    22801 atctcgaagc gccgcgcgcg cactctcaag aagatgatcc tcccgctcgt tgctgtcgct
    22861 ctgatgatgg ccgcgcacgc aatgagctac tgagctcgtg cgagctgtca tcgctctgct
    22921 gatcgccggg tgcgctcact caccgcccgt cgtcgagaag acgccgtgcc ctgcacccgc
    22981 accggcacct gcgccggtcg tcgcgcccgc cactgcagct gttcctgcag cgtgggacga
    23041 cgcgaaggtc atcgagaaga gccaagcatt cctcgccgct gcggacacgt acgcacgtcc
    23101 gtctgccgtc gaagatccgg caatgatcac gttctcgggc gggcgcacgt acgacgcggc
    23161 gatggtcaag cgcgtcgcga cgcggctcaa agaacatcat ctgccgccgc agacgcgcac
    23221 gtgtgacaag caacgcgtgc tgcgcaccga gtcgacgatg atgtacttcg accgctgcga
    23281 gatcacgacg cccgcgcacg acgacgtccc gcagctcacc tattccgagt acgac
//
LOCUS       DATLBZ010000318        25620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6140610, whole genome shotgun sequence.
ACCESSION   DATLBZ010000318 DATLBZ010000000
VERSION     DATLBZ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 25620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25620
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6140610"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /gene="prs"
                     /locus_tag="VMZ53_16680"
     CDS             complement(<1..718)
                     /gene="prs"
                     /locus_tag="VMZ53_16680"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HUS30146.1"
                     /translation="MAVVMAVPGNEDQAAQLASRLGASVVSAEVRRFPDGELYVRVHG
                     DVAGHDVALVGSLYPSPAERFMTVAFLASTLRDLGAKRVGLVAPYLAFMRQDHAFNPG
                     EGVTSGYFAKLVSHTVDWLVTVDPHLHRHNSLDGIYTIPTTIARSAPAIAKWIAKEIQ
                     QPILIGPDAESVQWVSAVADSCGAPFVILEKTRRGDRDVSIAGTLTGLNGHTPVLIDD
                     IVSTGKTLIEATRQLRAAGSL"
     gene            complement(732..1655)
                     /locus_tag="VMZ53_16685"
     CDS             complement(732..1655)
                     /locus_tag="VMZ53_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30147.1"
                     /translation="MKSIVLAALLFAVSIAHAEDEQPVDPTVNASELVIPGVDDVPDE
                     TAPAEAPKARLAPMPKRARETVKDDGEGKLEKKEDTVAGGTHWRVKTNAGAVHVWVPP
                     GYDRATAGTVVYVHGYWNDADSAWRDHALAKQFRASKQNALFIVPDAPQSNEDGVKWP
                     ALKDLRKAIGRANIKMPDGPVIVMGHSGAFRTIMQWVDHKLVEQIILLDAMYAGESKF
                     DAFIKSGKRADRHKLIVVGAGTAQSSESFVKSYKFAVAREKMPASYAGFTKRERGAKL
                     LYIRSQFEHNAIVTGGKVIPLLLRLTALKSL"
     gene            complement(1652..2848)
                     /locus_tag="VMZ53_16690"
     CDS             complement(1652..2848)
                     /locus_tag="VMZ53_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30148.1"
                     /translation="MFRGVALAVVLTAAASTASAEDAARDYIDDAKVFYRVVSCGGAD
                     QPVPAGLDQKTVDAHCTAMAKTYEYTKTKYIEPAQAFFANLRPKDLPTTVVYPFGGGD
                     LLSVLVTYPDAKDITTISLEHAGDPTRLAKLTKKAELRQHLAEFRAAVDGLLRLHDST
                     SENMMKLEVGGIPGQLSFHITGLTVHGYEPVSLKYFTINDDGTLHYLTQTEIDGLAKK
                     KAKKLRGKWVDTDFSEAFNNMELTFRKAGDAKAPLITHRHIAWSLDDKKFKDSPLQKY
                     LVSKGKVAAMTKAASYLLWNGYFSGIRDYLLDNMAWMASDATGVPPKHAKKKGFTQTT
                     YGTFKGAFLEEYDAGVNEQMVEMWAKQPKRKLPFRYGYPDSEKHVHMMITAPTAPPAP
                     KDEKTK"
     gene            complement(2899..3444)
                     /locus_tag="VMZ53_16695"
     CDS             complement(2899..3444)
                     /locus_tag="VMZ53_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HUS30149.1"
                     /translation="MTFADLKRLIPVPKLDGSRNLIRQGWDVLSIVPGGKSVFSRLVG
                     RAAPYTATIHASVIALRTGYAEVSMADKRAVRNHLDCVHAIALANLAEMAGNLALAYS
                     LPDDARFIVSGMEIEYVKKARGTITAVGEPPVPRTSMRAAYDVPVTLRDATGEEVAKA
                     ILHSLVGPKPGTVNDTRTNMN"
     gene            3522..4151
                     /locus_tag="VMZ53_16700"
     CDS             3522..4151
                     /locus_tag="VMZ53_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30150.1"
                     /translation="MTTTTRLSGVLLALVLSACSSGAKRRLTTQEMITADPLPLAKGA
                     KWKYAVTVKKFDPDTNKETTKTLDWTTEVVDARESNGVTAYRVKGWPTDLADFDQPSP
                     TPTERTLLRSGNSFMFGASPEPTIDGAEGWFTWPVMDGQKICPKAEMVYCWQVSAVET
                     GYALSFFTGPDEQTFELEPGTGVARFHYAHHGTTNEVDAKLTSYTKGRR"
     gene            complement(4156..4980)
                     /locus_tag="VMZ53_16705"
     CDS             complement(4156..4980)
                     /locus_tag="VMZ53_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30151.1"
                     /translation="MSTIAMRLAAIAATMESLPEAESPHRNTLARLRAGALATAGRGL
                     EIVSAAEATGLSVPVVALGLALRGHDGSLDAGLQHAVRVSIAYLVSPAAPTRAVAEQM
                     LGLAPGDGGPEIGTLAARLLRLIAFLPPRRALLVNDLAEVIRDEVGTNPSQELARRYL
                     GVLFTIFEAAGVGWLKEDAGLFVDGEQIVDAIEWLDANAPLPPPIAAGTARLERDGDS
                     MVVRAGDDVPIELLVPLARLVWPRERDGFVELSVAPRDLAAHGPELRSAIERATGS"
     gene            5004..5459
                     /locus_tag="VMZ53_16710"
     CDS             5004..5459
                     /locus_tag="VMZ53_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30152.1"
                     /translation="MRNARRWLLGVGGIVIVATVGLVVVRPRLMAAEARELMANDPCV
                     LQKGPPRPSFFRAIELADTASKYPGVDRDRTDANQSRCVATFASACIDIEGRAITAAI
                     RAQKPETEWRQYVDRDEQRVFCNAWAPLIELIKTGRETVAGCRYCDEKN"
     gene            complement(5456..7927)
                     /locus_tag="VMZ53_16715"
     CDS             complement(5456..7927)
                     /locus_tag="VMZ53_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HUS30153.1"
                     /translation="MAHQRDPQQAHAAVGDAETAHGEAQEAPASGTAYTAQGEIPPVI
                     SEFGAQGPSASDRAFGVAKKGVKGPGGQLPHLDAIQRSFGDHDVSGVQAHVGGNAAAS
                     AQQLGAEAYAVGDTVGFQQQPTLHTAAHEAAHVVQQRQGVSFKGVDSPMASHEQHADQ
                     VADAVVAGKSAKPLLDSAPQAAGGAVQRKAGAQGAAADYTGADETERVRLALKAAGDA
                     ARTGVATIKKGDDKLNAYFAESGAKHINQHLDMAFGLTAGAPVDKKAYGAEAAAAHEA
                     IDQFVTAAVSYLEEAELGAMMKRSDKLGTWGTGVKVKAHNKGFDKKDRNKAAMLALRS
                     VRTHVDDRMDAYTNPNDLTFTAGFALEDLSYAYDLLASEPANPALAADVELTTRSLGR
                     LYAVIQSLEKHGGSPVGGGWLSSIKLKANGLRNLAGLTPDGWIGDVVDGQAVAADTKP
                     PKKEAARRELTQWNAAAHEIVDDVRRYNSNNWTAFYGATSQNPILGWDANVFDQTASN
                     FVGNILTTLGENLIKKGSAAIAGALIGSAAGPAGTVIGFVIGLIIETVASVVYDYFTH
                     DNSKEDRAAADGSKKTAKMTEAKNEQFNEAAVKGRAEQNAAHSAAVGELDSASSQAKV
                     DAIGAEARAEKEKASGPKNHADRSLYKAMLTDWVKEHAGDEESANVDTSKAQWNDAAK
                     EVTGKENLNNTRNLYAYQCRHEWKKAGFKGDQVDAIVNPMIQNPDEKRDQGKYVKFKG
                     VVDAPTHIGYLNRTANRVSMGAYKVAPSEERMQQIEKNPGDVEVECVLDLTHADGTTY
                     VNNWLYTIDPAWEGGFKSGFALSPD"
     gene            8291..9022
                     /locus_tag="VMZ53_16720"
     CDS             8291..9022
                     /locus_tag="VMZ53_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30154.1"
                     /translation="MGWLDERTVVVLADGIVATVTVDGTRTEVAKLDQSYDTLVVTQA
                     GEAWAARCAEPPERRKPGPCNQEFLRVIPAPQAQSVKAPEGIVPHRVMRGSDELVRIP
                     FKEVAAPAGVELHQIPLSSPKGFIVTGISCTSPGGSSKFPDVAALPAMGYAASESRWV
                     LEKPPIYQVVAATQHRSHTAVQVNYFRPCEARPLDGFYAFGDGVWAEYLATRDHGTWT
                     FRVEDKVIGTVSGSAGLRPNHDVSP"
     gene            complement(9024..10346)
                     /gene="tolB"
                     /locus_tag="VMZ53_16725"
     CDS             complement(9024..10346)
                     /gene="tolB"
                     /locus_tag="VMZ53_16725"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HUS30155.1"
                     /translation="MAMLQVQRSAVILLALAAVASAQPAGKGSGSGASDGVVIDVSGT
                     KGGKYPIALPTAPENDAVAKEISQVESFDLNIAGPFKVLDPAGFLADLKAEGTGIEAQ
                     KWKDVGAYGVIKYKASADSIDFKLYEVSKGTKPSLEKSYKRSGTDTRTIVHKWCNEVV
                     KYYTGEAGFFGSKIVFTAKGKGKSAIMAMDFDGNNAYSVSNNSSTNILPSFSPTGGQV
                     AYTSFMRSNPDLYVAPAGGGRPKKISGQKGMNTGAAWSPDGSKLALTLSKDGNPEIYI
                     INAADGSIIKRLTNNRYIDTSPAWSPDGSQIAYVSDSSGGPQIFVMPASGGKATQVSF
                     NGNYNTTPVWSPKAGKKIIAYTTRDGGNYDIVTLDLDSKAMTRITQSQGSNEEPSFSP
                     NGRAIAFARTSGGGGAGIYIANADGTGTAVKVYSGSTTGVDWGPAPKD"
     gene            complement(10313..11020)
                     /locus_tag="VMZ53_16730"
     CDS             complement(10313..11020)
                     /locus_tag="VMZ53_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30156.1"
                     /translation="MSLKEPEMQILSIIGAVALTAGLFVPMLWFTDKAEADGPQLKDM
                     EAIEATIAYKKTPTKQPQKQTQPQPTIEKPEGVSHDENKKPPEKKPDEKKPDAAKVDP
                     NNPFGKFKRTDEDEGKPTTQPGDFNGDEKGFAPTSKGDPFFGRLRADMNFQFPEIAKA
                     SSIPVGCLHIQADGQIANTTFDPPIGQKGDDDLQTAAEAAMRELVKARRANPEPVPTH
                     LLGITTKWLCFKFSVQQ"
     gene            complement(11023..11487)
                     /locus_tag="VMZ53_16735"
     CDS             complement(11023..11487)
                     /locus_tag="VMZ53_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HUS30157.1"
                     /translation="MGMSSGGGDGELNSEINVTPMVDVMLVLLIIFMITAPMMNTGVS
                     LDLPQVTAQNIEDPEGKLVLSIGKDSKIYLGGTQVTWKDLEAKLKANERVQKEGALYI
                     EADNALPYGVVITAMAVAKNSGVAHVLMLTNPTENLKLDDLDQAIGRLPASK"
     gene            complement(11492..12211)
                     /gene="tolQ"
                     /locus_tag="VMZ53_16740"
     CDS             complement(11492..12211)
                     /gene="tolQ"
                     /locus_tag="VMZ53_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HUS30158.1"
                     /translation="MPGFSPTQVSWTLAAKLDIPRLFNQAHWIVQAVMGLLAVMFIIG
                     LYIIIYKWLYVGRAKAESDRFLEAFWKSRDIEQIYKQAQNLRNSPISQMFIAGYTELA
                     KLHSDEQLKDDREGNLANIERALRRAQTHETTKLESMVPFLATTGSAAPFIGLFGTVV
                     GILFAFLDIADSKEASAASIKTVGPHIAEALFATAIGLVAAIPAVMAFNYFQRRIKVL
                     RSEMDTFSQDYLNIIKRHFLR"
     gene            12834..13556
                     /locus_tag="VMZ53_16745"
     CDS             12834..13556
                     /locus_tag="VMZ53_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30159.1"
                     /translation="MKNLVLGSLIAVLASQAAGCVIESSDDTYVTASWSIKHLANNTT
                     IPCPPGYTTAALYNQPVNSDGTTVGSPTIDLFDCAANIGTSGPLAPGLYLSWIEIAND
                     NNTSVYAKSLSTPVDTSPGDQAASFTIYEDGGYFQLQWNLVGAQTNAPLTCASAGAAG
                     GVEVTATLSGTSNAASDQFDCENQYGVTDAYGTGSYTVSVAALNASDQSIGTAPTIPT
                     ANIGSANAVTNLGTINIPIDGK"
     gene            13637..14392
                     /locus_tag="VMZ53_16750"
     CDS             13637..14392
                     /locus_tag="VMZ53_16750"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUS30160.1"
                     /translation="MARFVNKRDWKQMRITRGALILAVLQVTMTIGWLCLGDTLRAQL
                     APWIVLTPRYLLEQGHVWTLVTAPFLEVGNLGLVSLVLSLLMLWMFVPTLERFWGTPR
                     FLRFAAITSVVGGLAGAAVGLALGHHDEAIVGLSPFVYACIVAFGILYARQPVEFSFY
                     LRMTGRQMMWGFIAFITLFVVLQQLWEIGASYAAAMLCATVMTSKRWSPGLMWKRWRI
                     ARARAKLSVIEGGALGAKPAKKKRAADEQRFLN"
     gene            14441..15922
                     /locus_tag="VMZ53_16755"
     CDS             14441..15922
                     /locus_tag="VMZ53_16755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS30161.1"
                     /translation="MEEGDPQFESGTGLTTLFEGEWATVRRLRKGKLVVVNGPDQGKE
                     IEILKPRVTGGRSIISDLVVQDKAVSGSHFEVSARDDGYRLRDLNSRNGIFVGDLRVR
                     EAYLRPGTVFRIGHTNIQFQSTQDVVEIELSKKDRFDMMLGSSPAMREIFAHLEKVAP
                     SDLTCLITGETGTGKEMVARALHNASSRKNKPFVVLDCGSIPRELIESTLFGHEKGSF
                     TGAVAQATGCFEQANGGTIFLDEIGELDISLQPKLLRVLEQREIKRVGGDRTYKVDVR
                     VLAATNRDLREEVNKGTFREDLYFRLSVVHVELPAMRERREDIPPLANHFLREIAQRR
                     GMTMSFSQDAMSAMMSHSWAGNVREMRNVVERAAALSDGPVITRADLVFGREMGPSVV
                     VSHDLAQAGAQAAKRAAAELAGVELPQQSGPAIFDASLLKAGLGFKQAKQTVVDSFEI
                     AYLQALMVRNDGNITRSAQEAGLTRYHLRELLKRHGLKGGDVE"
     gene            15922..16902
                     /locus_tag="VMZ53_16760"
     CDS             15922..16902
                     /locus_tag="VMZ53_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30162.1"
                     /translation="MPRAELDPALSLRVLGARELRYDDRPQLGEDRPGHVRAASGLAM
                     VGGRLAIIQDDCAFIGYVSDQVTAVPLPRGPNGRRRFEEAIGNKADKLDLEACVVVPG
                     DRTGEGQLGDELWAFGSGSTPMREKILIVGFTTRLHDASPFYRKLREELSAPSPHESF
                     RVAPVVNIEGAALVGKELLLFHRGNTGPHDPGPMVFRMSRAAFQKWLASEGVVPEIDG
                     AQHYDLGEVDGHRIGFTDAVAVGARVFFLAAAEASPNAIDDGRVLASRIGVIDDGAVR
                     MATLEIDGVPIKAEGLAFDPANPTRAWITIDPDDVATPAKLYEVELVGSW"
     gene            complement(16972..17172)
                     /locus_tag="VMZ53_16765"
     CDS             complement(16972..17172)
                     /locus_tag="VMZ53_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30163.1"
                     /translation="MNRTVLEYTQLLSRAFNGAIEVDGTRIVAHPSGTLIARTPNPMH
                     TQPVAPRAQPLTLDPENDWTNV"
     gene            complement(17246..18034)
                     /locus_tag="VMZ53_16770"
     CDS             complement(17246..18034)
                     /locus_tag="VMZ53_16770"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30164.1"
                     /translation="MARKKLGEMLVEAGVLSDQGLRMALNEQKRWGGTLGRTLVEMKL
                     VSEAELVRVLAAQLAVPTIDLDHLEIHPTVLELVPGDLAEQYSIVPFAQPMKFLDVAM
                     ADPTNLGVIDELRIRTQLNIRPYLAGPKMIERALAKHYRRGFGRAIYRRDVEVNLDNT
                     GDTISPGLTGPQHDRPDTAEIDAARRREGLRPAPRPSRPPPLLEPVPLAPNRDAEIEA
                     LQERISKLEALVERDEGVLRKVLALLIEKGVATREEILERIQDR"
     gene            complement(18044..19951)
                     /locus_tag="VMZ53_16775"
     CDS             complement(18044..19951)
                     /locus_tag="VMZ53_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30165.1"
                     /translation="MTRVTSSLLATVLALAACGDDPAVAYFKVPSAAANEDFYDLPWP
                     NNYWRKADGTLDLSQFPTNAVIANTVREIAQRDLDGFGKNAVMFVRFSGALDDTSLPS
                     PEASVTDAASVYVVDVDAMSPDKGKKTPVVVTFHEEGTQTMGGNRLAIRPFPGFPLRD
                     GTTYALVVTKRVHDSNGNTIQRDGDFSSLITSTGGSEAISVIRTTYEPLLSWLDEAGG
                     DERGDVIAASVFTTQHATKFGPALRKGVYGAPAPVATDLMKGPAGTAFTLFSGKYEQP
                     NFQAGLPAYISSGGEITIGSDGAAVVTRMEPMRFALSVPTGAVPTNGFPICIYQHGTG
                     GDWVSFLGDGTAERLAAQGIAVISTDQVLHGPRGAGTDPSIAFFNFNNPVAGRDNPLQ
                     GAADAWAMQRLVAGLTIPASLDGARDITFNMDKLFFFGHSQGGLTGPAYIAFEPTLKG
                     AVLSGTGGTFYLSTLTKTEPVNFPDLIATLIRDEPVNEDNPTLGLAQMAVERSDGVNY
                     APLMVREPPLGDNGMPLAPKNIFQTEGFTDHYSPNAVIEAFAVALGGDIVELPDQKEL
                     EGVTLRERGIMKPPFKNNVAGVTVALGQYKQAGTSDGHFVVFDVGSARKQSSQFLGTL
                     AATGQATVVSP"
     gene            complement(19999..20715)
                     /locus_tag="VMZ53_16780"
     CDS             complement(19999..20715)
                     /locus_tag="VMZ53_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30166.1"
                     /translation="MLASRAVLLLLVLAGGIAEADPRRVIGILEVRVDGVPRDIAAQF
                     QKNLEAQLDSREYWLAPSARMHELLSNSTKWSEGCVVGPCLMEVKVQTGAEVVLLAAI
                     HGDGTSFGYVVTLVRTDTGEYLAQEAERCDVCTVSEALTSATLAAVKLVTALPEKLPD
                     EAAEHRAQMDRVTKTAAHDKTGMQQHRKTLGTVLALSGIAVAAGGAALYFTQDHSSYG
                     LATAGVGCGALVGGLVTMTF"
     gene            complement(20715..21221)
                     /locus_tag="VMZ53_16785"
     CDS             complement(20715..21221)
                     /locus_tag="VMZ53_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30167.1"
                     /translation="MAEASKEPAAGAQGIYTSWDAFLKQAIREYYDRGWTTRKGNFIA
                     LLIASGSTVAGLAKDSVVDGSGTKKVAIGAAMAVALRIGLRYALGGPLSLVLSVAAGA
                     SMIAYFVRNQKDIVKKVSVYKTAIADNEKRYNEVQSGWRDGKYQVTDRNLMIDGLMKQ
                     FIGQVDEA"
     gene            21282..24068
                     /locus_tag="VMZ53_16790"
     CDS             21282..24068
                     /locus_tag="VMZ53_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS30168.1"
                     /translation="MRNRGALALFIVCALGTHASADLASGRDKLQGGDYKNAIAELSK
                     ITGKDQKAARILLARAQIATGDIAAAEGTLAALAKGTDAQAIEARILLNEVRWETGRG
                     ADARKDLEQLYKDKPDDRSVRTALAEVRFNQGAVADAKTLFELTIKEFDAQKLNLDDA
                     MQLYQLAIAAHYTAQFELANDSYRAALKINPRFTEIGVRWAALFSRKYANELAAQTLE
                     EVFKINPNHPDAHAAMAGVITETSYDLQAVDHHLDAALAINPKSVRALLVRASIEIDR
                     NEWDAAKKTLGQVLAINKEDLEALALSATISWLRDDKAAYEAEKQKAFAINSKYAELY
                     RIVSRSAVREHRYVEAIELEKEAIKLKPDYYEAMAGAGLGYLRLGNEKEGLEWLDKAY
                     KGDGYNVRTVNTLNLFENKIPKQYGFSSSKNFKIRYHNEEKPALQRYLEPTMERAFAD
                     MVRRYGFTPKTPVVLELYPDKEAYAVRTVGLPDVAALGVCFGQVITALSPMTGDINWG
                     MVLWHELGHVFAIQLSNSRVPRWFTEGLSEYETLIARPEWRRENDADLYGAIANKTLP
                     SIGDLNSEFMQPDQQAVVVAYFLSAVTVEWLANTYGFPKIVEALKLYGKGQETPEVLK
                     AITGKTIAQLDTEFHKYLDIRLAAYTNTFKLPTRGFDDVTKLEIAADAKPRDGAARAN
                     VALGYYYGGDAEKAGVAADKAIALDPKNAIARYIQAEIAMHSSDFVKAKQLYTSLVAD
                     GHDSYDMRARLAQLAQEDGDVAEVEKQLCAAKKLDPERSYPYQALSEIYKKQGKREQG
                     LAELEHYAFIEQMELAPLKELVLSYAELKAWAKVRLYGEMATYINPQDSEVLMSLAQA
                     YLELKQPDRALFAYDTVLVAKPEPRRPALVHLGRARAYAAQNKKADAKVALALAMKTE
                     PENAEVLALKAQLK"
     gene            complement(24072..24761)
                     /locus_tag="VMZ53_16795"
     CDS             complement(24072..24761)
                     /locus_tag="VMZ53_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS30169.1"
                     /translation="MPAAKTKPRPRKRAVKKPPEPAYRRLDNDERRAQLLALGRAAFA
                     AHPFDEVSIDDLAAKAGLSKGLFYYYFPTKRDLYSAAVEAVARELMTSVIANVAKANT
                     PRDRATAGVDAYLDHVEKQGPAFIALMRGGVGSDKKVATALDKVRAQMLDQFISGAPI
                     GAFLKKRPLGRIAIRGWIAMVETASVEWMSSQDIPREPLRDLLVDQLFDLLTRVLGQK
                     VSQTEFMTEKK"
     gene            24948..>25620
                     /locus_tag="VMZ53_16800"
     CDS             24948..>25620
                     /locus_tag="VMZ53_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30170.1"
                     /translation="MGPGPRPRARTQREAPPRRATTDDFALQAIARRELAAAAPAPNE
                     LPATARARGERKSSTRRQIEAMAQQEREAIARRELDAMAQRQREAIARKEREAITRRD
                     LGAAVSRPGALSPRREPGAAVGDAAVGGPGAISSRREHGAAIGDAVGDAAGGGAGAIS
                     SRREHGAAAAGGGSISARHEHGAAVGGAALGGAGAMSARHEHDAAVAGGGSIGARREH
                     GAAGGG"
BASE COUNT         4370 a         8246 c         8232 g         4772 t
ORIGIN      
        1 cgagactgcc agccgcccgc agctggcgcg tagcttcgat gagcgtcttg cctgtcgaga
       61 cgatgtcgtc gatgaggacg ggcgtgtgac cgttgagacc cgtgagcgtg ccggcgatcg
      121 aaacgtcacg atcaccgcgc ctggtcttct cgagaatgac aaacggcgcg ccgcagctgt
      181 cggcgactgc cgaaacccac tgcacgctct ccgcatcggg tccgatcaga atcggctgct
      241 gaatctcttt ggcgatccat ttcgcgatcg caggagcgct gcgcgcgatc gtggtcggaa
      301 tcgtgtagat gccgtcgagc gagttgtggc gatgcaggtg cggatcgacc gtcacgagcc
      361 aatcgaccgt gtgcgagacg agcttcgcga agtacccgga cgtgacgcct tcaccgggat
      421 tgaacgcgtg gtcctggcgc atgaacgcga ggtagggcgc gacgaggccg acgcgcttcg
      481 caccgagatc gcgcagcgtc gacgcgagga acgcaaccgt catgaagcgt tcggcaggcg
      541 acggatacag gctgcctacc agcgcgacgt catggccagc gacatcgccg tgcacgcgga
      601 cgtacagctc gccgtcggga aaccgccgga cctccgccga gaccaccgac gcgccgagcc
      661 gcgacgcgag ctgcgccgct tgatcctcgt tgcccggaac ggccatgacg actgccatgc
      721 cgtcgatggt actagagtga cttcagcgcc gtcagccgga gaagcagcgg aatgactttg
      781 ccgccggtca cgatcgcgtt gtgctcgaac tgcgaccgga tgtagagcag cttcgcgccg
      841 cgttcccgct tcgtgaaacc agcgtaagac gcgggcatct tctcgcgcgc gaccgcgaac
      901 ttgtagctct ttacgaagct ctctgacgat tgcgcggttc cggcaccgac gacgatcagc
      961 ttgtgccgat ccgcacgttt gccgctcttg atgaacgcgt cgaacttgga ctcgccggcg
     1021 tacatcgcgt cgagcaggat gatctgctcc acgagcttgt ggtcgaccca ctgcatgatc
     1081 gtgcgaaacg cgccggagtg gcccatgacg atcaccggac cgtcgggcat cttgatgttc
     1141 gcgcgaccga tcgccttgcg tagatctttg agcgccggcc acttcacgcc gtcctcattc
     1201 gactgcggcg catcgggcac gatgaacagc gcgttctgct tgctcgcgcg gaactgcttt
     1261 gcgagcgcgt gatcgcgcca cgcgctgtcg gcgtcgttcc agtaaccgtg cacgtacacg
     1321 acggtgccgg ccgtcgcgcg atcgtagcca ggcggaaccc acacgtgcac tgcgcccgca
     1381 ttggtcttca cacgccagtg cgtgccgccc gcgaccgtgt cttccttctt ctcgagcttg
     1441 ccctcgccgt cgtccttcac cgtctcgcgt gcgcgcttcg gcatcggggc gaggcgagcc
     1501 ttcggcgcct ctgcaggcgc ggtctcatcg ggcacgtcat cgacgcccgg aatcacgagc
     1561 tccgacgcgt tcaccgtcgg atcgacaggc tgttcgtcct ctgcatgcgc gatcgaaacg
     1621 gcaaacagca gcgcagcgag aactatcgac ttcatttcgt cttctcgtct tttggtgccg
     1681 ggggtgccgt cggcgccgtg atcatcatgt ggacgtgctt ctcgctatcg gggtagccgt
     1741 agcggaacgg aagcttgcgc ttgggctgct tggcccacat ctcgaccatc tgctcgttca
     1801 cgccggcgtc gtactcctcg aggaacgcgc ccttgaacgt gccgtacgtc gtctgcgtga
     1861 agcccttctt cttggcatgc ttcggcggaa cacccgtcgc gtccgacgcc atccacgcca
     1921 tgttgtcgag caggtaatcg cggatgcccg agaagtagcc gttccacaat agatagctcg
     1981 cggccttcgt catcgccgcg accttgccct tgctgacgag atacttctgc agcgggctgt
     2041 ccttgaactt cttgtcgtcg aggctccacg cgatgtggcg atgcgtgatg aggggagcct
     2101 tcgcgtcacc ggctttgcgg aacgtgagct ccatgttgtt gaacgcttcg gagaagtcgg
     2161 tgtcgaccca tttgccgcgg agcttcttcg ccttcttctt cgcgaggccg tcgatctcgg
     2221 tctgcgtcag gtagtgcagc gtgccgtcgt cattgatcgt gaagtacttg agcgacacgg
     2281 gctcgtagcc gtgcacggtg agccccgtga tgtgaaacga gagctggccg gggatgccgc
     2341 cgacctcgag cttcatcatg ttctcgctcg tcgagtcgtg caggcgaagc agaccatcga
     2401 cggcggcgcg gaactcggcg aggtgctgcc tgagctccgc ctttttcgtc agttttgcca
     2461 ggcgagtggg gtcccccgcg tgctcgagcg agatcgtcgt gatgtctttc gcatcgggat
     2521 acgtgacgag caccgagagc agatcgccgc cgccgaatgg atacacgacc gtcgtcggaa
     2581 gatccttcgg ccgcaggttc gcgaagaacg cctgcgcagg ctcgatgtac ttcgtcttcg
     2641 tgtactcgta cgtcttggcc atcgcagtgc agtgcgcgtc gaccgtcttc tgatcgaggc
     2701 ccgcgggcac cggctggtcg gcaccgccgc acgaaaccac gcgatagaag accttcgcgt
     2761 cgtcgatgta atcacgcgca gcgtcctccg cgctcgccgt gctggcggca gcggtgagaa
     2821 cgacagcgag ggcgactccg cggaacatga agcgtgtttt atccatacga cgatcggatc
     2881 gacgtagcta tttctcggct aattcatgtt cgtgcgggtg tcgttgacgg ttccgggctt
     2941 gggcccgacg aggctgtgca agattgcctt ggcaacctcc tcacccgttg cgtcgcggag
     3001 cgtcacggga acatcgtagg cggcgcgcat gcttgtgcgc ggaacgggtg gctcaccgac
     3061 cgcggtgatg gtcccgcgcg ccttcttcac gtactcgatt tccatgccgg aaacgatgaa
     3121 gcgagcgtca tcgggtagcg agtacgcgag ggcgagattc cccgccatct ccgcgaggtt
     3181 cgcgagcgcg atcgcatgca cgcagtcgag gtggttacgc accgcgcgct tgtcggccat
     3241 cgagacctcg gcgtagcccg tgcgaagtgc aatgacgctg gcgtggatcg tcgcggtgta
     3301 aggcgccgca cgcccgacga gccggctgaa caccgacttg ccaccgggca cgatcgacag
     3361 cacgtcccag ccttggcgaa tcaagttgcg actcccgtcg agcttcggga cgggtatcag
     3421 ccgcttcagg tcggcaaacg tcatcgggcg ggaccataca agatgtcagt ccggatctgc
     3481 tggctatttg tgctgtgcca cgacgggggt aggatggccc cgtgactacg accacccgcc
     3541 tctccggcgt cctcctcgca ctggttctga gtgcgtgtag cagtggcgcg aagcggcgcc
     3601 tcacgaccca agaaatgatc accgcagatc ctctgccgct cgcaaagggc gcgaagtgga
     3661 agtacgcggt gaccgtcaag aagttcgacc ccgacacgaa caaagagacg acgaagaccc
     3721 tcgattggac gacggaagtc gtcgacgcgc gagagtccaa cggtgtcacc gcgtatcgcg
     3781 tcaaaggctg gcccacggac ctcgccgatt tcgatcagcc gtcgccgacg ccgaccgagc
     3841 gaacactctt gcgctcgggc aacagcttca tgttcggcgc atcgccggag ccgacgatcg
     3901 atggcgccga gggttggttc acgtggcccg tgatggacgg ccagaagatc tgcccgaagg
     3961 ccgagatggt ctactgctgg caagtgtccg ccgtcgagac tggatacgcg ctctcgttct
     4021 tcacgggccc cgacgagcaa acgttcgagc tcgagcccgg caccggcgtt gcgcgctttc
     4081 actacgcgca tcacggcacg acgaacgaag tcgacgcgaa gctcacgtcg tacacgaagg
     4141 gccgacgctg atacgtcagc tgcccgttgc gcgctcgatc gcggatcgca gctctggacc
     4201 gtgggcagcg agatcgcgag gcgcaaccga cagctccacg aacccgtcgc gctccctcgg
     4261 ccataccaga cgcgcgagcg ggacgagcag ctcgatgggg acgtcgtcgc ccgctcgtac
     4321 gaccatcgag tcgccatcgc gttcgagtcg ggcagttcct gctgcgatcg gcggcggcag
     4381 tggcgcgttg gcatctagcc attcgatggc gtcgacgatc tgctcgccgt ccacgaacaa
     4441 gccagcatcc tctttcaacc agccaacacc ggcggcttcg aaaatcgtga acaagacccc
     4501 gagataacgg cgggcaagct cctggcttgg attcgtacca acctcatctc ggatgacttc
     4561 ggcgaggtca ttgaccaaga gggcccgacg cggagggaga aacgcgatca gccgcaacaa
     4621 gcgcgcggca agcgttccga tctcaggtcc gccgtcacct ggtgcgagac cgagcatttg
     4681 ctcggcgacc gcacgcgttg gtgcagcggg cgacaccaag tatgcgatcg agacacgcac
     4741 cgcatgttgc agcccggcgt cgagcgagcc gtcgtgcccg cgcagagcga gcccgagtgc
     4801 gaccacggga acgctaagac cggttgcttc cgcggcgctc acgatctcga ggccccgacc
     4861 tgctgtcgcg agcgcacccg ccctcaaacg cgccagcgtg ttgcgatgcg gggactcagc
     4921 ttcgggcaac gactccatcg tcgccgcgat tgccgccagg cgcattgcga tcgtgctcat
     4981 gctgacgcgt tatcattcca tagatgcgca acgcacgtcg atggctgctc ggtgtcggcg
     5041 gcatcgtgat cgtcgcgact gtcgggcttg tcgtggtgcg tccgcgcttg atggcggccg
     5101 aggcaaggga gttgatggcg aacgatccgt gcgtgttgca gaagggcccg ccgcgtccct
     5161 cgttctttcg cgcgatcgag ctcgccgaca ccgcgagcaa gtaccctggc gtcgaccgcg
     5221 atcgcacgga cgcaaatcag tcgcgctgcg tggccacatt cgcgtcggcg tgcatcgaca
     5281 tcgaagggcg cgcgatcacg gcggcgatcc gagctcagaa gccggagacc gaatggcggc
     5341 agtacgttga tcgcgacgag cagcgtgtgt tctgcaacgc atgggcgccg ctgatcgagc
     5401 tcataaaaac cggtcgcgag accgtcgcgg gctgtcgcta ctgcgatgaa aaaaactagt
     5461 cgggcgacaa cgcgaagccg gacttgaacc caccctccca ggccggatcg atcgtgtaga
     5521 gccagttgtt gacgtaggtc gtgccatccg cgtgcgtcag gtcgagcacg cattcgactt
     5581 caacatctcc tgggttcttc tcgatctgct gcatgcgttc ctctgacggc gcgaccttgt
     5641 acgcacccat cgagacccga tttgcagttc ggttcagata accgatatgc gttggcgcgt
     5701 cgacgacgcc tttgaacttc acgtacttcc cctggtcgcg cttctcatcg gggttttgga
     5761 tcatcggatt gacgatggcg tccacctgat cgcccttgaa ccccgccttc ttccattcat
     5821 ggcgacattg gtaggcgtag agattccgcg tgttgttgag attctctttg cccgtgactt
     5881 ccttggccgc gtcgttccat tgcgccttgg acgtatcgac gttcgcactt tcctcgtcgc
     5941 cggcgtgctc cttgacccag tccgtcaaca tcgccttgta caacgaacga tcggcgtggt
     6001 tcttcgggcc cgatgccttc tctttctctg cgcgtgcctc cgccccgatc gcatccacct
     6061 ttgcttgcga cgaagcgctg tccagctcgc ccaccgcagc actgtgcgcc gcgttctgct
     6121 cagctctgcc cttcacggca gcttcattga attgttcgtt cttcgcctcg gtcatcttgg
     6181 ccgttttctt cgagccatcg gccgctgcgc gatcttcttt cgagttgtcg tgggtgaagt
     6241 agtcgtacac gaccgacgcc actgtctcga tgatgagacc gatgacaaat ccgatcaccg
     6301 tacctgcggg tcccgcggct gaaccgatca acgcacccgc gatcgctgcc gaccccttct
     6361 tgatcaaatt ctcgccgaga gtcgtcagga tgttgcccac gaagttcgac gcggtctggt
     6421 cgaagacgtt cgcgtcccat ccaagaattg gattctggct cgtggctccg tagaaggcag
     6481 tccagttgtt gctgttgtag cggcggacgt cgtcgacgat ctcgtgcgcc gccgcgttcc
     6541 attgcgtcag ctcgcgacgt gcggcctctt tcttcggcgg cttcgtatcc gcggcgaccg
     6601 cttgaccgtc gaccacatcg ccaatccagc cgtcaggcgt caagccggcg agattgcgca
     6661 acccgttcgc tttgagcttg atcgagctga gccaaccgcc accgacgggt gacccgccgt
     6721 gtttctccag gctctggatg accgcgtaaa ggcgtcccag cgagcgcgtc gtgagctcga
     6781 catcggccgc caatgcgggg tttgctggct cgcttgcgag caggtcgtac gcgtagctga
     6841 gatcctcgag cgcgaagccc gccgtgaagg tgaggtcatt gggattggtg tacgcatcca
     6901 tccggtcgtc gacgtgcgtc ctgacggatc gcaacgccag catggcggcc ttgttgcgat
     6961 cctttttgtc gaatcccttg ttgtgagctt ttaccttcac gcccgttccc cacgtgccca
     7021 gcttgtcgct gcgcttcatc atggctccga gctcggcctc ttctaggtag ctgaccgcgg
     7081 cggtaacaaa ctgatcgatg gcttcgtgtg ccgcggccgc ttcggcaccg taggccttct
     7141 tgtcgacagg cgcgccggcc gtcaaaccga aagccatgtc gaggtgttga ttgatgtgct
     7201 tcgcgcctga ctccgcgaag taggcgttca atttatcgtc gcctttcttg atggtcgcga
     7261 cgccggttcg cgctgcgtcg ccggccgcct tcagcgcgag cctgacgcgc tcggtctcgt
     7321 cggcacccgt gtaatccgct gccgctcctt gggcgccggc cttgcgctga acggcgccgc
     7381 ccgcagcttg tggtgcgctg tcgagcaacg gctttgccga cttgccagcg acgaccgcat
     7441 ccgccacctg atcggcgtgc tgctcgtgcg atgccatcgg tgagtcgacg cccttgaacg
     7501 agacaccttg acgttgctgc acgacgtgcg ccgcttcgtg cgcggccgtg tgaagagttg
     7561 gttgctgctg aaaaccaact gtatccccga ccgcgtacgc ttctgcgccg agctgctggg
     7621 cggaggctgc ggcgttgccg ccaacgtgcg cttgcacgcc ggatacgtcg tgatcgccga
     7681 acgagcgctg aatcgcgtcg aggtgcggca actggccgcc aggccccttc acacctttct
     7741 ttgcgacgcc gaatgcacgg tcggaagcgg acgggccttg cgcaccgaac tccgagatga
     7801 ccggcggaat ctcgccctgc gccgtgtatg cggtgccgct tgcgggagcc tcttgtgctt
     7861 ctccgtgcgc ggtctctgca tcgccgacgg cggcatgcgc ttgctgcgga tctcgttggt
     7921 gcgccatggc gatgctcctc gggctagctt gatacggccc ggctgactcg aaacttcctc
     7981 tgaggtttcc ggctcgtggc tctgctatgc gacatgataa tggtcttggc tcatggtgtg
     8041 gaagcaatgg atattgctgc ttgctgctgt cgggtgcgga aacagcaatc ccagcgctcc
     8101 ccatcgaggc agcggcggcg gatcgaacga gcgcgtcctg ccatccgacg cggcccgttc
     8161 acgtgttcca gagggtggct actacttcgt cgttctccgc ggcgggagct tcgcgatcgc
     8221 gaaactgctc gacgaccacg tggaagtcgt tcgcgatgca ggtccactct ctgaatctgg
     8281 tctgtcgggg gtggggtggc tggacgagcg gaccgtcgtg gttcttgccg acggaatcgt
     8341 cgcgacggtc accgtcgacg gcacgcgcac cgaggtcgcg aagctcgatc agagctacga
     8401 cacgctcgtg gtcacgcagg caggcgaggc gtgggcggcc cgctgcgccg aaccgccgga
     8461 acgtcgcaaa ccggggccgt gcaaccaaga gttcctccgc gtcattccag cgccgcaagc
     8521 gcagtccgtc aaagctccag agggcattgt cccgcaccgt gtgatgcgtg gctccgacga
     8581 gctcgtgcgc atcccgttca aggaagtcgc tgcgcctgcc ggcgtcgagc ttcaccagat
     8641 cccgttgtcg agcccgaaag ggttcatcgt tacgggaatc tcatgcacgt cgcctggagg
     8701 atcgtcgaag ttccccgacg tcgctgcgct gcctgcgatg ggctacgccg cgagcgagtc
     8761 gcgatgggtc ctcgagaagc cgccgatcta tcaggtggtc gccgcgaccc aacaccgatc
     8821 gcacacggcc gtgcaggtca attacttccg accatgtgaa gcacgtccgc tagacggatt
     8881 ctatgcgttc ggcgacggcg tgtgggccga gtatctcgcc acgcgtgatc acggaacgtg
     8941 gacattccgc gtcgaggaca aagtgatcgg tacagtctcc ggatcggctg ggctgcgccc
     9001 gaatcacgac gtctcgccgt aactcagtct ttgggcgccg ggccccagtc gacgcctgtc
     9061 gtcgaaccgg agtagacctt cacggctgta ccagtgccgt cggcattcgc gatgtagatg
     9121 ccagcaccac cgccaccgct cgtgcgagca aatgcgatcg cgcgtccgtt cggcgagaac
     9181 gacggctctt cgttcgagcc ttggctctgc gtgatacgcg tcatcgcctt gctgtcgaga
     9241 tcgagcgtga cgatgtcgta gttgccgccg tcgcgcgtcg tgtacgcgat gatcttcttg
     9301 cccgccttcg gggaccacac gggcgtcgtg ttgtagttgc cgttgaacga cacctgcgtc
     9361 gctttgccgc cgctcgccgg catcacgaag atctgcgggc cgccgctcga gtcgctgaca
     9421 tacgcgatct gcgatccgtc gggcgaccac gccggcgacg tatcgatgta gcggttgttc
     9481 gtcagacgct tgatgatcga tccgtctgcg gcgttgatga tgtagatctc tgggttgccg
     9541 tccttcgaca gcgtcagcgc gagcttgctg ccgtcgggcg accacgcagc gccggtgttc
     9601 atgcccttct ggccgctgat cttcttcggg cgaccaccgc cggcaggcgc gacgtagaga
     9661 tccgggttcg agcgcatgaa gctcgtgtac gcgacctgtc cgccggtcgg agagaacgac
     9721 ggcaggatgt tcgtcgagct gttgttcgag accgagtagg cgttgttgcc gtcgaagtcc
     9781 atcgccatga tggccgactt gcctttgccc ttcgcggtga agacgatctt cgagccgaag
     9841 aagccggctt cgcccgtgta gtacttgacg acttcgttgc accacttgtg gacgatcgtt
     9901 cgcgtgtcgg tgcccgagcg cttgtagctc ttctcgagcg acggcttcgt gcccttgctg
     9961 acttcgtaga gcttgaagtc gatcgaatcg gcgctcgcct tgtacttgat cacgccgtac
    10021 gcgccgacgt ccttccactt ctgcgcttcg atgccggtgc cttcggcttt gaggtcggcg
    10081 aggaagcccg ccggatcgag gaccttgaac ggacctgcga tgttgaggtc gaacgattcg
    10141 acctgggaga tttctttggc gactgcgtcg ttctccggtg ccgtcggcag cgcgatcgga
    10201 tacttcccgc ccttcgtgcc gctgacgtcg atgacgacgc cgtcgctcgc gccgctgccg
    10261 ctgcctttac cggcaggttg tgccgaggcg actgctgcga gcgcgaggag gattactgct
    10321 gaacgctgaa cttgaagcat agccatttgg tcgtgatgcc gagcaagtga gtcggcaccg
    10381 gttcggggtt cgcgcgacga gcctttacga gctctctcat cgcggcttcc gcggcagtct
    10441 gcagatcgtc atcgcccttt tgcccgatcg gaggatcgaa cgtcgtgttg gcgatttgac
    10501 catccgcttg gatgtgtaga cagcccactg gaatgctcga tgctttggcg atctccggga
    10561 actgaaaatt catgtccgcg cgcaagcgcc cgaaaaatgg gtcgcctttg ctcgtgggtg
    10621 cgaagccctt ttcatcgccg ttgaagtcgc cgggctgcgt cgttggcttg ccctcatctt
    10681 catccgtgcg tttgaatttt ccgaacgggt tgttcgggtc caccttcgcg gcgtcgggct
    10741 tcttttcgtc gggcttcttc tcgggcggct tcttgttctc gtcgtgcgag acgccttcgg
    10801 gcttttcgat cgtcggctgc ggctgcgttt gcttctgcgg ctgcttcgtc ggcgtcttct
    10861 tgtacgcgat cgtcgcttcg atcgcttcca tgtctttgag ctgagggccg tcggcttcgg
    10921 ctttatcggt gaaccagagc atcggcacga acagacccgc cgtcagcgcc accgcgccga
    10981 tgatcgagag gatctgcatc tctggttctt tcagcgacat gattacttag acgcaggaag
    11041 ccggccgatg gcttggtcga gatcgtcgag cttgaggttt tccgtcgggt tcgtgagcat
    11101 cagcacgtgc gcgacgccgg aatttttcgc gacggccatc gccgtgatca cgacgccgta
    11161 cggcagagca ttatcggcct cgatgtacag cgcgccctct ttctgcactc gttcgttcgc
    11221 cttgagcttg gcctctagat ccttccacgt cacctgggtt ccgcccaggt agatcttcga
    11281 gtccttgcca atcgagagga ccagcttgcc ctcgggatcc tcgatgttct gcgcggtgac
    11341 ctgcggcaga tcgagcgaca cgcccgtgtt catcatcggc gcggtgatca tgaagatgat
    11401 cagcagcacc agcatgacgt cgaccatcgg cgtcacgttg atctcgctgt tcagctcccc
    11461 gtcgccgccg cctgatgaca tccccatcgc gctacctcag gaagtgccgc ttgatgatgt
    11521 tgaggtagtc ctgcgagaac gtatccatct cggagcgcag gaccttgatg cgacgctgga
    11581 agtagttgaa cgccatcacg gcgggaatcg cagcgacgag accgatcgcg gtggcgaaca
    11641 gtgcttcggc gatgtgcggg ccgacggtct tgatcgacgc ggcagacgct tctttcgaat
    11701 cggcgatgtc gaggaacgcg aacaagatgc cgacgacggt gccgaacagg ccgatgaacg
    11761 gcgccgccga tcccgtggtc gcgaggaagg gaaccatcga ctcgagcttc gtcgtctcgt
    11821 gggtctgcgc acggcgaagc gcacgctcga tgttggcgag gttgccttcg cgatcgtcct
    11881 tgagctgctc gtccgagtgg agcttcgcga gctccgtgta gcccgcgatg aacatctgcg
    11941 agatggggct gtttcggaga ttctgcgctt gcttgtagat ctgctcgatg tcgcgcgact
    12001 tccagaacgc ttcgaggaag cgatcggact cggccttcgc gcggccgaca tacagccact
    12061 tgtagatgat gatgtagagg ccgatgatga acatcacggc cagaaggccc atcaccgctt
    12121 ggacgatcca gtgggcctgg ttgaacagcc gcgggatatc gagcttcgcc gcgagtgtcc
    12181 agcttacttg cgtcggcgaa aaccctggca taatgaggcc ttcgcattac cacgcacgat
    12241 gcgcgccggt caaatgtccc acccctgcgc cgtagccact ctgccacggg ctgttgcgct
    12301 cgtactgtgg atggctgttc acggctgcgc gaacgtccca ggcggagccg tcgagctttc
    12361 gtggaagtta cgggccgcgt cgggctcggg tgaatcgttc ttatcgtgtg aaagcgggat
    12421 cgctggcacg ggcaacgtcg agaagatccg gcttgattgg gacgtcgacg gctcgctcgg
    12481 ctcacacgag tggcgatgcg cggatgatca cggtgcgacg ggattcgagc tgccagaagg
    12541 cgttgcgctg ttgcgcgtct ctccagtgtg cgtggatcac gatgcggagg ccgattcgta
    12601 caccgcgcca gcacccgagc aacgtcacgt gatcgcggga gataccatca gcttgggagg
    12661 cgtcgagctc ttgctcgaag tgtcctcatg cgatcgccaa cggtgtattt gccagtgaga
    12721 ttgcggtggg tcacacggcc ctggcccgtt ccttgcctta gtcttcgcgt tgaagagata
    12781 ctgcggccgc gcgtccatat gcggtccact acgacccgag gggaaaaaac caaatgaaga
    12841 accttgtcct aggaagcttg atcgccgttc tcgccagcca ggcggctggc tgcgttatcg
    12901 agagcagcga cgacacgtac gtgaccgcga gctggtcgat caaacacctc gcgaacaaca
    12961 cgaccatccc gtgccccccc ggctatacga cggcggcgct gtacaaccag ccggtcaaca
    13021 gtgacggcac cacggtcggc tcgccgacca tcgacctgtt cgactgtgcc gcgaacatcg
    13081 gcaccagcgg acctctcgct cccggccttt atctgagctg gatcgagatc gcgaacgaca
    13141 acaacacgtc ggtctacgcg aagtcgctct cgacgccggt cgacacgtcg cccggtgacc
    13201 aggccgcttc gttcacgatc tacgaggatg gcggctactt ccagctgcag tggaacctag
    13261 tcggtgcgca gaccaacgct ccgctgacgt gcgcgtcggc tggcgcggcg ggtggcgtcg
    13321 aagtgaccgc gaccctgtcc ggcaccagca acgcggcgag cgatcagttc gactgcgaga
    13381 accagtacgg cgtcacggac gcgtacggca cgggctcgta caccgtctcg gtggccgcgc
    13441 tcaacgcatc ggaccagtcg atcggcaccg cgccgacgat cccgactgcc aacatcggta
    13501 gcgcgaacgc cgtgaccaac ctgggcacga tcaacatccc gatcgacggg aaataacgca
    13561 aacctcgccg tagtaggcac gacgcgcgcc cgccccattc ggcgggcgtt gtcgttttcg
    13621 gtgctaaggt ttgctcgtgg cccggttcgt caacaagcgc gactggaagc aaatgaggat
    13681 tacgcgcggc gcgctgatcc tcgccgtgct gcaggtaacg atgacgatcg gctggctctg
    13741 cctgggcgat acgcttcggg ctcagctcgc gccctggatc gtcttgacgc cacgctacct
    13801 gctggagcag ggccacgtct ggacgctcgt caccgcgccg ttcctcgagg tcggtaacct
    13861 cggcctcgtc tcgctggtcc tgagcctgct gatgctctgg atgttcgtcc cgacgctcga
    13921 gcgcttctgg ggcaccccgc gcttcttgcg cttcgccgcg atcacgtccg tggtcggtgg
    13981 gctggcaggc gccgcggtcg gtctcgcgct cggccaccat gacgaagcga tcgtaggact
    14041 gtcgccattt gtatacgcct gcatcgtcgc gttcggcatt ctctacgcgc gtcagcccgt
    14101 cgagttctcg ttctacctgc gcatgacggg ccgccagatg atgtggggct tcatcgcctt
    14161 catcacgctg ttcgtcgtgc ttcagcaact ttgggagatc ggcgcttcgt acgctgcggc
    14221 gatgctctgc gcgacggtga tgacgtcgaa gcggtggagc cccggactca tgtggaagcg
    14281 ctggcgaatt gcgcgcgcga gggcaaagct ctcggtgatc gagggtggcg ctctcggggc
    14341 aaagcctgcg aagaagaagc gcgccgccga cgagcagcgc tttctcaact aggcctacac
    14401 gtaagcctgc gctcccgggt cgtgataacg tcgaccccaa atggaagagg gtgatccgca
    14461 gttcgaatct ggcactggcc tgacgacgct cttcgagggc gagtgggcca ctgttcgccg
    14521 gctgcgcaag ggcaagctgg tcgtggtgaa cggcccggat cagggcaagg aaatcgagat
    14581 cctcaagccg cgcgtcaccg gtggccgcag catcatcagc gacctcgtcg ttcaggacaa
    14641 agcggtctcg ggctcccact tcgaagtcag tgcgcgcgac gacggctacc gcctccgcga
    14701 tttgaacagc cgcaacggca tcttcgtcgg cgacctgcgc gtccgcgaag cgtacctgcg
    14761 cccgggcacc gtcttccgca tcggccatac gaacatccag ttccagtcga cgcaggatgt
    14821 cgtcgagatc gagctgtcga agaaggaccg cttcgacatg atgctgggct cgtccccagc
    14881 gatgcgcgaa atcttcgcgc acctcgagaa ggtcgcgccg agcgatctca cgtgcctcat
    14941 cacgggcgag accggcaccg gtaaagagat ggtcgcgcgt gcgctccaca acgcgagctc
    15001 gcgcaagaac aagccgttcg tcgtgctcga ttgcggatcg atcccgcgcg agctgatcga
    15061 gtcgacgctg tttggtcacg agaagggcag cttcacgggc gccgtcgcgc aagccaccgg
    15121 ctgcttcgag caggcgaacg gcggcacgat cttcctcgac gaaatcggcg agctcgacat
    15181 ctcgctgcag cccaagctcc tccgcgtgct cgagcagcgc gagatcaagc gcgtcggcgg
    15241 cgatcgcacg tacaaggtcg acgttcgcgt gctcgcagcg acgaaccgcg atctgcgcga
    15301 agaggtcaac aagggcacgt tccgcgaaga cttgtacttc cgcctctcgg tcgtccacgt
    15361 cgagctcccc gcgatgcgcg agcgtcgcga agacatccca ccgctggcca atcacttctt
    15421 gcgcgagatc gcgcagcgcc gcggcatgac gatgtcgttc tcgcaggacg cgatgtccgc
    15481 gatgatgagc cacagctggg ccggcaacgt tcgcgagatg cgcaacgtcg tcgagcgagc
    15541 agccgcgctc tccgatggcc ccgtgatcac gcgcgccgac ctcgtgttcg gtcgcgagat
    15601 gggcccatcg gtcgtcgtca gtcacgacct cgcacaagcc ggcgcacaag ccgccaagcg
    15661 cgcagctgcc gagctcgcgg gcgtcgagct gccacagcag tcaggtcccg caatcttcga
    15721 cgcgtcactg ctcaaagccg gcctcggctt caagcaagcg aagcagaccg tcgtcgacag
    15781 cttcgagatc gcgtacctgc aagcgctcat ggtgcgcaac gacggcaaca tcacgcggtc
    15841 ggcccaagaa gccggtctca cgcgctacca cttgcgcgag ctcttgaagc gtcacggcct
    15901 caagggcggc gacgtcgagt agtgcctcgc gcagagctcg atccagcgct gtcattgcgc
    15961 gtgctcggtg cgcgcgagct gcgttacgac gaccggccgc agctcggtga agatcgaccg
    16021 ggacacgtgc gcgcggcatc ggggctcgcg atggtcggcg gccggctcgc gatcatccag
    16081 gacgactgcg cgttcatcgg ctacgtctcc gatcaggtga ctgcggtgcc gctgccacgc
    16141 gggccgaacg gccgccgccg cttcgaggaa gcgatcggca acaaagccga taagctcgat
    16201 ctcgaggcat gcgtcgtcgt gccgggcgat cgcacgggcg aaggccagct cggcgacgag
    16261 ctgtgggcat tcggctcggg ctcgacgccc atgcgcgaga agatcctgat cgttggattc
    16321 acgacgcgcc tgcacgatgc atcgccgttc tatcgaaagc tgcgcgaaga gctcagcgca
    16381 ccgagcccgc acgagtcgtt ccgcgtcgcg ccggtcgtga acatcgaggg tgccgcgctc
    16441 gtcggcaaag agctgctgct gtttcaccgt ggcaacacgg gcccgcacga cccggggccg
    16501 atggtgtttc ggatgtcgcg cgccgcgttc cagaagtggc tcgcgagcga aggcgtcgtg
    16561 ccggagatcg atggggcgca gcactacgat ctcggcgagg tcgacggcca tcgcatcggt
    16621 ttcacggatg cggtcgcggt cggtgcacgc gtattctttc tcgccgccgc cgaagcgtcg
    16681 cccaatgcga tcgatgacgg ccgcgtgctc gcatctcgca tcggcgtgat cgacgacggc
    16741 gccgtgcgca tggcaacgct cgagatcgat ggcgtgccga tcaaggccga gggcctggcg
    16801 ttcgatccgg cgaaccccac gcgcgcgtgg atcacgatcg atcccgatga tgtcgcgacg
    16861 ccggcgaagc tgtacgaagt cgagctcgtc ggttcctggt aacgctgccc gaaaaaaccg
    16921 aacggacccg ctagcccccc agcggatccg ttcgctcgtg tttcgtatgg gctagacgtt
    16981 cgtccagtcg ttctctggat cgagcgtcag cggctgtgcg cgtggcgcga caggctgcgt
    17041 gtgcatcggg ttcggcgtgc gcgcgatcag cgtcccgctg gggtgcgcga cgatccgagt
    17101 cccgtctact tcgatcgcgc cgttgaaggc ccgcgagagc aactgggtgt actcgagcac
    17161 cgtccggttc acgacgcctc ttctacacga ggcgtattca ctccggaacc gaaaaagtga
    17221 aggaggggga tctgctcccg gtgttctaac ggtcctggat ccgctccagg atctcctcgc
    17281 gggtcgcaac gcccttttcg atgaggagag ccaacacctt gcgcagcacg ccctcgtcgc
    17341 gctcgacgag cgcttcgagc ttggagatgc gctcctgcag cgcttcgatc tcggcatctc
    17401 gattcggcgc gagcggcacc ggctcgagca acggcggcgg acgcgatgga cgcggggcag
    17461 ggcgcagacc ttcgcgccgg cgcgcggcat cgatctccgc ggtatcgggc cggtcgtgtt
    17521 gcggccccgt aaggcccggc gagatcgtat cgcccgtgtt gtcgagattg acctcgacgt
    17581 cgcggcgata gatcgcgcga ccaaagccgc gtcggtagtg cttggcgagc gcgcgctcga
    17641 tcatcttggg gcccgcaaga tacgggcgga tgttgagctg cgtgcgaatg cgaagctcgt
    17701 cgatcacgcc gaggttcgtc gggtcggcca tcgcgacatc gaggaacttc atcggctgcg
    17761 cgaacggcac gatgctgtac tgctcggcga gatcaccggg cacgagctcg agcaccgtcg
    17821 ggtgaatctc gaggtgatcg agatcgatcg tcggcactgc gagctgcgcc gcgagcacgc
    17881 gcacgagctc cgcttcactg acgagcttca tctcgacgag cgtgcgaccg agcgtgccgc
    17941 cccatcgctt ctgctcgttg agcgccatgc gcaggccctg gtcgctcagc acgcccgcct
    18001 ccaccagcat ctccccgagc ttcttacgcg ccatccgtac ctcttacggg gacactaccg
    18061 tggcctggcc cgtcgcggcc agcgtgccga gaaactggct cgactgtttg cgagccgatc
    18121 ccacgtcgaa gacgacgaag tggccgtcgc tcgtgccggc ttgcttgtac tggccgagcg
    18181 cgaccgtgac gccggcgacg ttgttcttga acggcggctt catgatcccg cgctcgcgca
    18241 gcgtcacgcc ttcgagctct ttttgatcgg gaagctcgac gatgtcgccg ccgagcgcga
    18301 ccgcgaacgc ctcgatcacc gcgttcgggg agtagtgatc ggtgaagcct tccgtctgga
    18361 agatgttctt cggggcgagc ggcatcccgt tgtcgccgag cggcggctcg cggaccatga
    18421 gcggcgcgta gttcacgccg tcggatcgct cgaccgccat ctgcgcgagc ccgagcgtcg
    18481 ggttgtcctc gttgaccggc tcgtcgcgga tgagggtcgc gatgagatcg ggaaagttca
    18541 ccggctccgt cttcgtgagc gtgctcaggt agaacgtgcc gccggtgccc gagaggacgg
    18601 cgcctttgag cgtcggctcg aatgcgatgt acgccggtcc ggtcaggccg ccttgcgagt
    18661 ggccgaagaa gaacagcttg tccatgttga acgtgatgtc gcgcgcgccg tctagcgacg
    18721 ctggaatggt caagcccgcg acgaggcgct gcatcgccca tgcatcggcc gcgccttgga
    18781 gcgggttgtc tcgtccggca accggattgt tgaagttgaa gaacgcgatc gatggatcgg
    18841 ttcccgcacc gcgcggcccg tggagcactt ggtccgtcga gatcaccgcg atgccttgcg
    18901 cggcgaggcg ctctgccgtg ccgtcgccga ggaacgagac ccagtcgccg ccggtgccgt
    18961 gctggtaaat gcagatcgga aacccgttcg tgggcactgc gccggtcggc acgctgagcg
    19021 cgaaccgcat cggctccatg cgcgtcacga ccgcggcacc gtcggagcca atcgtgattt
    19081 caccgccgct cgagatgtat gcgggcagac cggcctggaa gttcggctgc tcgtacttgc
    19141 cgctgaacag cgtgaatgcc gtgcccgcag gccccttcat cagatccgtc gccaccggcg
    19201 ccggtgcgcc atacacgccc ttgcgcaacg ccgggccgaa cttcgtcgcg tgctgcgtcg
    19261 tgaagacgct cgctgcgatg acatcgccgc gctcgtcacc gccggcttcg tcgagccacg
    19321 acagcaatgg ctcgtacgtc gtgcggatga ccgagatggc ttcgctgcca cccgtgctcg
    19381 tgatcagcga cgagaaatca ccgtcgcgct ggatcgtatt gccgttgctg tcgtgcacgc
    19441 gcttggtgac gacgagcgcg tacgtcgtgc cgtcgcgcag cgggaagccg gggaacggac
    19501 gaatcgcgag gcgattgccg cccatcgtct gcgtgccctc ttcgtggaac gtcacgacga
    19561 ccggcgtctt cttgcccttg tcgggagaca tcgcgtcgac gtcgacgacg tacaccgacg
    19621 ctgcatcggt caccgacgcc tcgggcgacg gtagcgacgt gtcgtcgagc gcgccggaga
    19681 accggacgaa catgaccgcg ttcttgccaa agccgtcgag gtcgcgctgc gcgatctctc
    19741 ggaccgtgtt cgcgatgacg gcgttcgtcg gaaactgcga gagatcgagc gtgccgtcgg
    19801 ccttgcgcca atagttgttc ggccacggca gatcgtagaa gtcctcgttc gctgcagccg
    19861 acggaacctt gaagtacgcg accgccggat catcgccgca cgctgctagc gcgagtaccg
    19921 tcgccagcaa gctagaagtc accctcgtca tcgcggcgac cgtaacaccg acgggcccga
    19981 cgcgaggtga ccgaaatctc agaacgtcat cgtgacaaga ccgccgacga gagctccgca
    20041 accgacgccg gctgtcgcta gaccgtagct cgagtggtcc tgcgtgaaat acagcgcggc
    20101 accgcccgcg gcgactgcaa ttcccgacag cgcgagcacc gtcccgagcg tcttgcgatg
    20161 ctgctgcatt ccagtcttgt cgtgcgccgc cgtcttcgtc acgcggtcca tctgcgcgcg
    20221 gtgctcggcg gcttcgtcgg gcagcttctc ggggagcgcc gtgacgagct tcaccgcggc
    20281 gagcgtcgcg ctcgtgaggg cttcgctgac cgtgcagacg tcgcagcgtt ctgcctcctg
    20341 cgcgaggtat tcgccggtat cggtgcggac gagcgttacg acgtagccaa agctcgtgcc
    20401 gtcgccatgg atcgcagcga ggagcacgac ctccgcgccg gtctggacct tcacttccat
    20461 gaggcacggc ccgacgacgc agccctcgct ccacttcgtc gagttgctga gcagctcgtg
    20521 catgcgtgcc gacggagcga gccagtactc gcggctgtcg agctgcgctt cgaggttctt
    20581 ctggaattgg gcggcgatgt cgcgcggaac accgtcgacg cgaacctcga ggattccgat
    20641 gacacgacgc ggatctgcct cggcgatgcc gccggcgagt acgaggagga gaaggaccgc
    20701 tcgcgaagcg agcatcaggc ttcgtcgact tgaccgatga actgcttcat caggccatcg
    20761 atcatcaaat tgcggtcggt gacctggtac ttgccgtcgc gccaacctga ctggacttcg
    20821 ttgtagcgct tctcgttgtc ggcgatcgcc gtcttgtaga cgctcacctt cttgacgatg
    20881 tctttctggt tgcgcacgaa gtacgcgatc atcgaggcgc cggctgcgac gctgagcacg
    20941 aggctgagcg ggccgccgag cgcgtagcga aggccgatgc ggagtgcgac cgccatcgcc
    21001 gcgccaatcg cgaccttctt cgtgcccgag ccgtcgacga ccgagtcctt cgcgaggcct
    21061 gccaccgtcg agcccgacgc gatcagaagc gcgatgaaat tgcccttccg ggtcgtccaa
    21121 ccgcggtcgt agtactcgcg gatcgcctgc ttcaggaatg cgtcccagga cgtgtaaatc
    21181 ccctgcgcgc ctgccgccgg ttccttagag gcttctgcca cgtcattcag cttaccattc
    21241 gggtttgaca ggggtagggc cgtcgcacag attcgaggga gatgcggaat cggggcgctc
    21301 tcgcgctttt catcgtctgt gccctgggca cgcacgcaag cgcggacctc gctagcggtc
    21361 gcgacaagct ccaaggcggc gactacaaga acgcgatcgc cgagctgtcg aagatcaccg
    21421 gcaaggacca gaaggctgct cgcattctgc ttgcgcgcgc gcagatcgcg acaggcgaca
    21481 tcgctgcggc ggaaggcacg ctcgccgcgc tcgcgaaggg caccgacgcg caagcgatcg
    21541 aagcgcggat cctgctgaac gaggtgcgct gggagaccgg ccgcggcgcc gacgcgcgca
    21601 aagatctcga gcagctctac aaagataagc ccgacgatcg ctcggttcgc accgcgctcg
    21661 cggaggttcg cttcaaccaa ggcgcggtcg ccgacgcaaa gacgttgttc gagctgacga
    21721 tcaaagagtt cgacgcccag aagctcaacc tcgacgatgc gatgcagctc taccagctcg
    21781 cgatcgccgc gcactacacc gcgcagttcg agctcgcgaa cgattcgtat cgcgcggcgc
    21841 tgaagatcaa cccgcgcttc accgagatcg gtgttcgatg ggcggccctg ttttcccgca
    21901 agtacgcgaa cgagctcgcg gcgcagacgc tcgaagaagt cttcaagatc aacccgaacc
    21961 atcccgacgc gcacgcggcg atggcaggcg tgatcaccga gacgagctac gacctgcagg
    22021 ccgtcgatca tcacctcgac gcggcgctcg cgatcaatcc caagagcgtt cgcgcactcc
    22081 tcgtccgcgc ttcgatcgag atcgatcgca acgagtggga cgcagcgaag aagacgctcg
    22141 gtcaggtcct cgcgatcaac aaggaagacc tcgaagcgct cgcgctgtcg gcgacgatct
    22201 cgtggctgcg cgacgacaag gccgcgtacg aagcggagaa gcagaaggcg tttgcgatca
    22261 actcgaagta cgccgagctg tatcgcatcg tgtcgcgctc ggccgttcgc gagcatcgct
    22321 acgtcgaagc gatcgagctc gagaaggaag cgatcaagct caagcccgac tactacgagg
    22381 cgatggcggg cgctggcctc ggctatctcc gactcggcaa cgagaaggaa ggcctcgagt
    22441 ggctcgacaa ggcctacaag ggcgacggct acaacgttcg caccgtcaac acgctcaacc
    22501 tgttcgagaa caagattccg aagcagtacg gattctcgtc gagcaagaac ttcaagatcc
    22561 gctaccacaa cgaagagaag ccagcgctcc agcgctatct cgagccgacg atggagcgcg
    22621 cgttcgcgga catggtccgc cgctacggct tcacgcccaa gacgccggtc gtgctcgagc
    22681 tctatcccga caaagaggcg tacgcggttc gcaccgtcgg tctgcccgat gtcgccgcgc
    22741 tcggcgtgtg cttcggccaa gtgatcacag cgctgtcacc gatgaccggc gacatcaact
    22801 ggggaatggt gctctggcac gagctcggtc acgtgttcgc gatccagctg tcgaatagcc
    22861 gcgtgccgcg ctggttcacc gaaggcctct cggaatacga gacgctgatc gcccgcccgg
    22921 aatggcgccg cgagaacgac gcggacctct acggcgcgat cgcgaacaag acgctgccgt
    22981 cgatcggcga tctcaactcg gaattcatgc agcccgacca gcaggcggtc gtcgtcgcgt
    23041 acttcctgtc cgcagtcacc gtcgagtggc tcgcgaacac gtacggcttc ccgaagatcg
    23101 tcgaggcgct caagctctac gggaaaggcc aggagacgcc cgaggtgctc aaggcgatca
    23161 cgggcaagac gatcgcgcag ctcgacaccg agtttcacaa gtacctcgac attcgcctcg
    23221 ccgcgtacac gaacacgttc aagctgccga cccgcggctt cgacgacgtc acgaagctcg
    23281 agatcgcggc tgacgccaag ccgcgagatg gcgccgctcg cgcgaacgtg gcgctcggct
    23341 actactacgg cggtgatgcg gagaaggcgg gcgtcgcggc ggacaaagcc atcgcgctcg
    23401 atccgaagaa cgcgatcgcg cgctacatcc aagcggagat cgcgatgcac tcgtcggact
    23461 tcgtcaaggc gaagcagctc tacacgagcc tcgtcgccga cggtcacgac agctacgaca
    23521 tgcgcgcccg cctcgcgcag ctcgcgcaag aagacggcga cgtcgccgaa gtcgagaagc
    23581 agctgtgcgc cgcgaagaaa ctcgatcccg agcgcagcta cccgtaccaa gccctctccg
    23641 agatctacaa gaagcaaggc aagcgcgaac aaggcctcgc cgagctggag cactacgcat
    23701 tcatcgagca gatggagctg gcgccgctca aggagctcgt cctctcgtac gccgagctca
    23761 aggcgtgggc gaaagtgcgc ctctacggcg agatggcgac gtacattaac ccgcaagact
    23821 ccgaagtgct gatgagcctc gcgcaggcgt acctcgagct gaagcaaccc gacagagcgc
    23881 tgttcgcgta cgacaccgtg ctcgtcgcaa agcccgagcc ccggcgtccc gcgcttgtcc
    23941 acctcggccg cgcgcgcgcg tacgcggcgc agaacaagaa ggccgacgcg aaggtagcgc
    24001 tcgcgctcgc gatgaagacc gagcccgaga acgcagaagt gctcgcgctc aaggcgcagc
    24061 tgaagtagcg gctacttctt ctcggtcatg aactcggtct gcgagacctt ttgcccgagc
    24121 acgcgcgtca gtagatcgaa cagctgatcg acgagcagat cgcgcagcgg ctcgcgcgga
    24181 atgtcttgcg atgacatcca ctcgacgctc gcggtctcga ccatcgcgat ccagccgcga
    24241 atcgcaattc gcccgagcgg ccgcttcttg aggaacgcgc cgatgggcgc gccgctgatg
    24301 aactggtcga gcatctgcgc gcggaccttg tcgagcgcgg tcgcgacctt cttatcggag
    24361 ccgacgccgc cgcgcatcag cgcgataaac gccggacctt gcttctcgac gtgatcgagg
    24421 taggcgtcga cgcccgccgt cgcgcgatca cgcggcgtgt tggccttcgc gacgttcgcg
    24481 atgacgctcg tcatcagctc gcgcgccact gcttcgaccg ccgcgctgta gaggtcgcgc
    24541 ttcgtcggga agtagtagta gaagaggccc ttcgacagcc cggccttggc cgcaaggtca
    24601 tcgatcgaga cttcgtcgaa gggatgcgca gcaaacgcgg ctcggccgag ggccaggagc
    24661 tgcgcacgcc gctcgtcgtt gtcgaggcgg cggtaggcgg gctcgggcgg cttcttgacc
    24721 gcgcgtttgc gcgggcgggg tttcgtcttg gcggcaggca cgggtgattg ggcgcgtggt
    24781 accgcgaacg gcggtcggac cacaacgatt tgttgagtgc gcgtcagcaa ctgaacatgg
    24841 cttgttgacg gtccgtcaac aagtgatgcg tccttgttga cgctgtctca acaagtgtca
    24901 gggctgcaca ctggtgtggg caggtcgacg cgcgcagggt gaacgccatg ggccccggac
    24961 cgcgaccgcg agcgcgaacg cagcgagagg caccgccgag gcgcgccacg acggacgact
    25021 tcgcgctcca ggccatcgct cgccgagagc tagcggccgc tgcgcccgcg cccaatgagc
    25081 tgccggcgac ggcgcgcgcg cgcggcgaac gcaagtcgag cacgcgtcga cagatcgagg
    25141 cgatggcgca gcaggagcgc gaggcgatcg cgcgcaggga gctcgacgcg atggcgcagc
    25201 gccagcgcga ggcgatcgcg cgcaaagagc gcgaggcgat cactcggcgc gacctcggcg
    25261 cggcagtatc ccgtcccggc gctctcagcc cgcggcgcga gcctggtgcc gcagtcggcg
    25321 atgcggcggt cggcggcccg ggtgcgatca gctctcggcg cgagcacggc gcggcgatcg
    25381 gcgacgcggt cggcgatgcg gccggcggcg gcgcgggtgc gatcagctct cggcgcgagc
    25441 acggtgccgc ggcagccggc ggtggttcga tcagcgctcg gcacgagcac ggtgcggcgg
    25501 tcggcggcgc ggcgctcggc ggcgcggggg cgatgagcgc tcggcacgag cacgatgccg
    25561 cggtagccgg cggtggttcg atcggcgctc ggcgcgagca cggtgcggcg ggcggcggcg
//
LOCUS       DATLBZ010000319        16260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6141914, whole genome shotgun sequence.
ACCESSION   DATLBZ010000319 DATLBZ010000000
VERSION     DATLBZ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16260
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6141914"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VMZ53_16805"
     CDS             <1..820
                     /locus_tag="VMZ53_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase"
                     /protein_id="HUS30171.1"
                     /translation="ASAGAKVSVAGWPPALGIFKTMLERGKFDESLKLANGEKMQFAK
                     IYALDAEYDRMEDVPQEVRENRRYRDLGDFTISGLAAALKADIGDGQLDICVHSLANG
                     SEVKKPMLETSRKGYLSAVSASAYSMVSLVSHLGPIMRAKGAFCSLSYMASERVVPGY
                     GGGMSSAKAALESDTRVLAFEAGRKWGHRVNVISAGPYASRAASAIGFIQQMLDYAKR
                     NAALPEEITAEEVGATAAFLCSPLASGITGSTVYVDKGYQAMGIAVDREGPKLG"
     gene            813..1457
                     /locus_tag="VMZ53_16810"
     CDS             813..1457
                     /locus_tag="VMZ53_16810"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUS30172.1"
                     /translation="MAEEAKPWRVLGCKGCGSAIVEAALVLAGIPYEREEADYDTKEG
                     HDRLAELNPLAQVPTLILPDGTVMTESAAIVLYVDGLMPDLGLVPPLRDPLRREFLRW
                     LMFLVAAVYPTFTYGDDPKKWTPDGADELRQSTDEHRQSLWRLVEGAVRGPWFLGDRP
                     SALDLYVSVMTRWRPRRAWFAEHCPRLHTIATAVDEDARLTNVWATNFDAPGAG"
     gene            1546..2823
                     /locus_tag="VMZ53_16815"
     CDS             1546..2823
                     /locus_tag="VMZ53_16815"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HUS30173.1"
                     /translation="MSARWLILLLLAAPACGVTEDYVDGPDDDAWLDGKADGASAVDV
                     ASTSLDVDLTAKSAVATIELEKYGNVSLEAGGLTIKKVTDARGNRRFKVIGGKLLVSN
                     VRSPMVVEYTFAQHANADGLLPGGSTVLWPYWCGNLFPCHSQPADGTTFTLNLDGVPA
                     NKQAIYPASIDAESPPYMLAWAVGSYTKKELGTTTAGTKVSTYWLPNGQTAALAGTKH
                     LVAAFDWYEKTIGPYSFGKDVASVSVVWGEGMYGGMEHHPYWHVARDAMNDEVTHVHE
                     AAHGWFGDGVRLRCWEDFVLSEGTVSYLAARALGQTAGATMEAKVWADYKTELDDAIA
                     EGGAPAWPTGCGQIDILKDKLFTNLPYMQGAFFYKDVAAQIGADKLDKIIGDFYKAHR
                     NKPAGMQDMIDAIKAASGFDPTPIAQARLRKKF"
     gene            2826..5639
                     /locus_tag="VMZ53_16820"
     CDS             2826..5639
                     /locus_tag="VMZ53_16820"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30174.1"
                     /translation="MQCPDDNALVAMADHGLPTAQFAHLEEHIDGCESCRKIVAAALA
                     SRSLAVGTPRPPSGNDVAEALVGTLDVSISDRYVVEAVLGKGGMGTVYLAHDKTLGRD
                     VALKLHHRADHAGAAERLRREAMAMAKLAHPNVVTVFEVATVDERLYVAMEYVRGETL
                     RGWLAQKKRSWREIIALLVEAGQGLAAAHDAGLIHRDFKPENVLVGDDGRPRVGDFGL
                     ARVGAAPASGLRISGSGPQRAPGSGPQRTPTAPPSKSDSKPGAASGLELADTALTKEG
                     TVLGTPAYMAPEQLSGAEVDARCDQFAFAVVAWECLYGKRPFAGMDREKRAEAPRSDV
                     PVRVRRVLERGLAIDPAARFADMPALLAALRTSSRPRTAQWIAASLAGVAVIGGVAYA
                     GAGMYRDRERHAACELEGDRVASVLRPEQQVAIRMSFLATRSPMADGAYEHAMSVLTP
                     YAKSLGAKTTQACLTADEPMHAARARCLSARKNRLATLADGFAHATKGKTTVSRAAEI
                     AWNVAHTDPCSDEDPVLTERKHAPTDPVLAKALDDVYAVRDAGKYDKELEAATALLAK
                     ARAAGDRETELDALLAQAMAQDALEKPERAKTFDEVAALAEALGRDVTAAGAYATMAA
                     NAARDQQDFTAAHRYLGLARAKLDRLGGKNIATRGDLFIIEAKIFMDEYRWAEGEAAG
                     RQGVALLEQALGPNHPKVGIATGTWSQLLRMVDKNDEALAQSERTLDIFTKAYGADHP
                     TTAGSSLNLAQSLIDAKRYDEARERLLSADKVFMRTFGPNHPVHISAWSNLATVEQLQ
                     EHWDLALSYDRRVLAMMEALGGPDSADASGAHRDIAKVLALSNHPHEALAEQQRAVSI
                     LEKLGEDGEGRMIGAQVELGEMQLELGNKSAAKVSLHRVLDLAAKHPNMADPVLDVKR
                     AQELLAYAEGRTPMPGSAKSASK"
     gene            complement(5645..7084)
                     /locus_tag="VMZ53_16825"
     CDS             complement(5645..7084)
                     /locus_tag="VMZ53_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HUS30175.1"
                     /translation="MGEVELQRSVVKLFTVVKEPNYYKPWELAYQHTSGGSACIIDGN
                     RILTNAHVIAHQLFVQASKPGDSKKYVARVLHVDHDTETALLTVDDPVFFEGTHPVTF
                     GDLPHRNAKVTVYGFPVGGDELCITAGVVSRIEVRTYTHSQRNLLALQTDAAINPGNS
                     GGPVFMDEKLIGIAFQSYKRKDLERSGYVVPTHVIKHMLEDLEDGRIDGVPDLGVYWQ
                     KIENDALRRFLGLRSDDTGVRVSRVLHSSSAHGVLEVDDVITSIEGTAVACDGSVPLR
                     PGDRVRFEHLIARHQVGRELDLGVLRAGKPLHLRVTLKKFVSLVPPPQPERRPTYLVF
                     GGLVFTVLTHDYMHDWEWAKEHHRYMNFYYEVMPSERRKQVVIIHQVLAHEINLGYHQ
                     VQGAVVERVNGVDITEIRDVVRALEQPTSGFHVIETDFHGSRRASFSSDFHHAYGTRI
                     VLDAEKVTAAHKAILEQHGVPNDRSADLR"
     gene            complement(7096..7290)
                     /locus_tag="VMZ53_16830"
     CDS             complement(7096..7290)
                     /locus_tag="VMZ53_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30176.1"
                     /translation="MTVDPAACPHTERREGVCVACGDCLHEVILNGVCFLCGTADLDP
                     IALSPKKPGVIPADRLVRKK"
     gene            7333..10248
                     /locus_tag="VMZ53_16835"
     CDS             7333..10248
                     /locus_tag="VMZ53_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30177.1"
                     /translation="MPIRVMPAVWLAVVVVAVGSVAACGNGKGKEPATSPPLTPGVVP
                     TAVAAAPPSQPPSDRLHHHYPIPVDLTNVEELDLAADGERIVAGGEQGGVIVVERTSR
                     KVLRRIAAPWVDSVAISADGAHVVVVGDKKAKLVDVATGKELATHAAQGTVARCGANG
                     WLVARDKQADVLDAQFKQVATLATPDGFGIHGTVACGGGRAVLVAKHALVVFDLASAK
                     QLVRIDVPTDGDANGLVDVVVGTKYIATISNAGSVWLYDVAGKLLATAPIIAGQASRL
                     ALSADESLLAAGNVILAPPDLAIRKRMDSGGVAAFTPDAAWLVTARNHDIDVLDVAAL
                     DTDPVDGHYDTGSSLAIDPSQAYLASGNGGRVFLWALDKATPGIGTGPLLAILDGHAI
                     GTFDNVSVAFHPDGKRLVSAGYATVVVWDLATRSPLARWSVQTDESYESELGVRVDDR
                     HIVVSGSNYVYVYDATTYKQLARLGSFKTEVMDGSVDNVALHPDGKHVAVTSQGGALA
                     VFDRVSGKRVAQRPAEKEAAERFPIFTPDGKLLIELASTYGRTLTWPKLDSAATFEAP
                     WASDAAWSADGKRLAVGSWNGLGLSVFERAGASLRKKWELQTPWHTAAVAWDNKRGVI
                     YTTGADESIHSWDAATGKHVASWVGAGGDGIDPLVLVGDTLLVYQSEINMLRFFDLAK
                     LEQTAHLDELYLEDDTEPRLIGDRLLVWTSPGKLARFQRDGTTWKALSTWNPPEDYGW
                     AGIGISADGKLAWAIHGETDANDQQTGELYTIDTSTLAVVSKQRAKNFGGAQMRVDPS
                     GKYVAFITGNDEADKLDVWTLSPLKRVRTLQSADGFRDFDFVGIDEIALDGDDPKRWS
                     ITTGKSRLDCGGGFHNTLGYSADHKRDVIIRGSSIRVRTLADCKEDAFFYDGERTAAV
                     DEKRLVVSNRGAITVWDLAKHEPVAILRALRDGLWSARTAGHYTPPAHAPMPPAKTP"
     gene            complement(10251..12293)
                     /gene="recD"
                     /locus_tag="VMZ53_16840"
     CDS             complement(10251..12293)
                     /gene="recD"
                     /locus_tag="VMZ53_16840"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HUS30178.1"
                     /translation="MSTTPPSLHPRGSLRAKFAHVGDHDGIFRKLGVGARRCDLGDEG
                     LYLAEEIVAADRWLGGSDPEALGILVLALMIAQRQGSSRLPLDDSRARSPSGAMPAES
                     PAPTKARLRTLVTEIMRVAGLELDTNRILKRIKDLTSAPSFNSVIGTGDARLPLIVDD
                     GCLYTERSRYLELRVAKRLAARLAAPRSLPKADMIADLVARPGKGTLSAEQLRAVELA
                     LSGSLAVVTGGPGTGKTLVAAAIVRGFARLGVPPIQIALAAQTGKAANRLTEMIGMQL
                     AAVASPADADRALVTSPPAAQTLHRLLGYRGDGFAHHAQSPLPVGALIVDEASMVDLE
                     LMDALLDALPASVPLILIGDAHQLPAIDAGQILADLAEPDGVGAGCVATLEHSFRMDA
                     ADPRGRAVLEAARAVRAGEAQRLTEKNGLAALRTPGTLKWSGAEWVDTTASAAGASKS
                     KSIEPVHAVAAALWHHFDGPLAQRLASETVFRFSDGRVDPAQQHELDDLWTRLGRARL
                     LTVTRGMPTGSLALNAFVHQLALERMTVTGRPEFVPGEPVMITANDYQRGLYNGDQGM
                     IIRADEGSGAHRYRAVFRVQGQLVPFAIEALRDRLELAWALTVHKSQGSELDALALVL
                     PEDEHLPLLTRELLYTGITRARTSAVLCGSRAAIVAAGKRGALRHSGLATRLRALR"
     gene            complement(12290..15628)
                     /locus_tag="VMZ53_16845"
     CDS             complement(12290..15628)
                     /locus_tag="VMZ53_16845"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HUS30179.1"
                     /translation="MRPDSLPPASDRLVVVEASAGTGKTFFLEHRVVDLVLAGAELSQ
                     ILLVTFTDKAVAELRMRIRDLLDRMARADIDQVAPSTWNIDDEARARLRAAVTAFDHA
                     PIFTIHAFCHRILIEDAFAARRLFDQTQVADEVAFDTAFNALLRERFATHSPDKELLA
                     AYLEWGKTVDNLRDLLLKCARADARPRRSLDPQRAVEVLGQLRAMLGTADERERFLHT
                     TSWGRGQRYAADWVDEAGRVLDRARGDDPAVALAACDELRDVAEKIVKYAKGPVANTL
                     IESMKVMSLGEAIASELLPHVVKRIGTDKAEHGMFDYDDMLDLVREALHGPRGSELAT
                     RLRTRMPWVMIDEFQDTDPVQWDIFRTVWMHPDAKGLTIVGDPKQAIYGFRGADVATY
                     LDAKHELLRNGATQVDLDVNRRSTAELVEAVNALLAGPPLGPLMDKTITYDHPVKASG
                     DVTCEGSRPPLTIFSLEGGGSRERDPDRDALALAISSEIERLRDRPHAWQSRGKAPPF
                     TLGHCMVLTRTGKESLQIASVLRSRGLACALVESDKLFETREAAELAALLEAIAAPRD
                     RSARMRALHTRFFDVPWAELMRVVDASDHHPLIARLFDWAALAGRREYEQLFRRVIED
                     SHFAERALVLGGGERALVNTWHLIELLLEEVARSRCDLHELTLRLRRWINEHEELADD
                     RDVQRAETDADAIRILTIHKAKGLEAPYVFLFGCATGGPKSKVGVLRDGAGRTLHVDT
                     NDDAIKQQLEMEADAENQRLAYVALTRAQIRLYVPLYGGKPPDKTAMYVPVQRCATHL
                     LKINHPLVETVHVGVGVAPDPQAPADALATFDVPAPPTITPLAPLAPARLGLAMLSYT
                     RLAHDLEAAALEPGELPHAIDPAEFDVDDRNAEARASLGPDDLPPGADAGLLLHAIFE
                     HADLATARAAKDVDAWRANPVVERLLVDQAREHGIAEQCLPHAARIVHTTLTRQLALT
                     DGTELPPLVEATAIARELEFTYPMPSAPDAPPNAPPRGLVKGFIDAIVAWNDELWVLD
                     YKSDLLGGDDLASAATDRMHEKYGVQARLYAVAADRMRGNRRLAGLLFAFVRHDIVVP
                     LRITDEILAHWTGWLASLRTEVPR"
     gene            complement(15687..>16260)
                     /locus_tag="VMZ53_16850"
     CDS             complement(15687..>16260)
                     /locus_tag="VMZ53_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30180.1"
                     /translation="FGRSSSPMAELMPALELSLASGRTVRLVGQTELLVRRGTGYASV
                     IPLIGKADKRSRHHLRGALDHVVLAAAGLSTGSHEHLLLDPEGTVRSVQHAPWSQADA
                     REFLTSLVGELLDQSHGYLLPFDHLVNGYAGKPPGRIYGDAITSLLGFGPIDRIDGLV
                     APPDPGSIAKRRLGPLVVRMTGDHSLGGSE"
BASE COUNT         2791 a         5317 c         5572 g         2580 t
ORIGIN      
        1 cgcatcggcc ggcgcgaagg tctcggtcgc cggatggccg cccgccctcg gcatcttcaa
       61 gacgatgctc gagcgcggca agttcgacga atcgctgaag ctcgcgaacg gcgagaagat
      121 gcagttcgcg aagatctacg cactcgatgc ggagtacgac cgcatggaag acgtgccgca
      181 ggaggtgcgc gagaatcggc gctatcgcga cctcggcgac ttcacgatct ccggccttgc
      241 cgccgcgctg aaagcggaca tcggcgatgg tcagctcgac atctgcgtgc attcgctcgc
      301 gaacggcagc gaagtgaaaa agccgatgct cgagacgtcg cgcaagggat acctcagcgc
      361 ggtgagcgcg agcgcgtact cgatggtctc gctcgtgagc caccttggac cgatcatgcg
      421 tgcgaagggc gcgttctgct cgctctcgta catggcgagt gagcgagtgg tgcccggtta
      481 tggcggcggc atgtcgtcgg cgaaggccgc gctcgagagc gacacgcgcg tgctcgcgtt
      541 cgaagcggga cgcaagtggg gccaccgcgt gaacgtaatc agcgccgggc cgtatgcatc
      601 gcgcgcggcg agcgcgatcg gtttcatcca gcagatgctc gactacgcga agcgcaatgc
      661 agcgctcccc gaggagatca cggccgaaga agtcggcgcg acggcggcat tcttgtgcag
      721 ccccctcgcg agcggcatca cgggctcgac ggtctacgtc gacaagggct atcaggcgat
      781 gggcatcgcc gtcgatcgcg aggggcccaa gcttggctga ggaagccaaa ccgtggcgcg
      841 tgctcggctg caaaggctgc ggctcggcaa tcgtcgaagc ggcgctcgtg ctcgcgggca
      901 ttccgtacga gcgtgaagaa gcggactacg acacgaagga aggccacgat cgcctcgccg
      961 agctgaaccc gctcgcgcaa gtgccgacgc tgatcttgcc cgatggcacc gtgatgaccg
     1021 agagcgctgc gatcgtgctc tacgtcgatg ggctcatgcc cgacctcggg ctcgtgccgc
     1081 ctctgcgcga tccgctgcgc cgcgaatttc tgcgctggct gatgttcctc gtcgccgcgg
     1141 tctacccgac gttcacgtat ggcgacgatc cgaagaagtg gacgccggac ggcgccgacg
     1201 agcttcgcca atcgaccgac gagcatcgcc aatcgctgtg gcgtctcgtc gaaggtgccg
     1261 tgcgtgggcc gtggtttctc ggcgatcggc cgagcgcact cgacctctac gtctcggtga
     1321 tgacccgctg gcggccgcgt cgcgcgtggt tcgcagaaca ctgcccgcgc ctccacacga
     1381 tcgcgactgc cgtcgacgaa gatgcccgcc tgaccaacgt ctgggcgacg aacttcgacg
     1441 cacccggcgc cggctagcaa gctagccagc ggccttcgcg tcgcacccta agcctgcgag
     1501 atccggtggc ccgaccatgg ccttcccctt gctgctagcg ggggtatgtc cgcgcgctgg
     1561 ctgatcctgt tgctgcttgc agctcccgcc tgcggcgtga ccgaagacta cgtcgacgga
     1621 cccgacgacg acgcgtggct cgacggcaag gccgatggcg cgagcgcggt cgatgtcgcg
     1681 tcgacgagcc tcgatgtcga tctcacggcg aagtcggcgg tcgcgacgat cgagctcgag
     1741 aaatacggca acgtctcgct cgaagcgggt ggcctcacga tcaagaaagt caccgacgct
     1801 cgcggcaatc gccgcttcaa agtgatcggc ggcaagctgc tcgtgtcgaa cgtgcgcagc
     1861 ccgatggtcg tcgagtacac gttcgcgcag cacgcgaatg ccgatggcct tctgcccggc
     1921 ggctcgaccg tgctgtggcc gtattggtgc ggcaatctgt ttccgtgtca cagccagccg
     1981 gccgatggca cgacgttcac gctgaacctc gacggcgtgc ctgcgaacaa gcaagcgatc
     2041 tatcccgcgt cgatcgacgc cgagtcgcca ccgtacatgc tcgcgtgggc ggtcggcagc
     2101 tacacgaaga aggagctcgg cacgacgacg gcgggcacga aggtctcgac gtactggctg
     2161 ccgaacgggc agaccgcagc gctcgcgggc acgaagcacc tcgtcgcggc gttcgattgg
     2221 tacgagaaga cgatcggtcc gtactcgttt ggcaaagacg tcgcgtcggt ctccgtcgtc
     2281 tggggcgaag gcatgtacgg cggcatggag catcatccgt actggcacgt cgcgcgcgat
     2341 gcgatgaacg atgaagtcac gcacgtgcac gaagccgcgc acggttggtt cggcgacggc
     2401 gttcgcctgc ggtgttggga agacttcgtg ctctccgagg gcacggtcag ctatctcgcg
     2461 gctcgtgcgc tcggccaaac cgctggcgcg acgatggaag cgaaagtgtg ggcggactac
     2521 aagacggagc tcgacgacgc gatcgccgag ggtggcgcac ctgcgtggcc gacgggctgc
     2581 ggccagatcg acatcctgaa ggacaagctg ttcacgaacc tgccgtacat gcagggcgcg
     2641 ttcttctaca aagacgtcgc tgcgcagatc ggtgccgaca agctcgacaa gatcatcggc
     2701 gacttctaca aagcgcaccg caacaagcca gccggcatgc aggacatgat cgatgcgatc
     2761 aaggccgcga gcggattcga tccgacgccg atcgcgcagg cacggcttcg caagaagttc
     2821 taacgatgca gtgtcccgac gataacgcgc tcgtcgcgat ggccgatcac ggacttccga
     2881 ctgcgcagtt cgcgcatctc gaggagcata tcgatggctg cgagtcgtgt cgcaagatcg
     2941 tggcggccgc gttggcgtcg cgctcgctcg cagtcggtac gccgcgcccg ccgagcggga
     3001 acgacgtcgc cgaagcgctc gtcggtacgc tcgatgtttc gattagcgat cgctacgtgg
     3061 tcgaagccgt gctcggcaaa ggcggcatgg gcacggtcta tctcgcgcac gacaagacgc
     3121 tgggacgcga tgtcgcgctg aagctccacc atcgcgccga tcatgcgggc gcagcggagc
     3181 gacttcgtcg cgaagcgatg gcgatggcca agctcgcgca tccgaacgtc gtcacggtat
     3241 tcgaagtcgc gaccgtcgac gagcgcctct acgtcgcaat ggagtacgtg cgcggcgaga
     3301 cgctgcgcgg atggctcgca caaaagaagc ggagctggcg ggagatcatc gcgctgctcg
     3361 tcgaagcagg gcagggtctc gctgctgcac atgacgccgg tttgattcat cgcgacttca
     3421 agccggagaa cgtcctcgtc ggcgacgatg gccgcccacg cgtcggcgac ttcggtctcg
     3481 cgcgcgtcgg tgctgcgccc gcctccggcc tgcgaatctc cggctcgggc ccacagcgcg
     3541 ctccaggctc cggcccgcag cgaacaccca ccgcgccgcc gagcaagtcc gattcgaagc
     3601 caggtgctgc cagtggtctc gagctcgcgg ataccgcgct gaccaaagaa ggcacggttc
     3661 tcgggacgcc cgcgtacatg gcaccggagc agctctcggg cgcagaggtc gacgcgcgct
     3721 gcgatcagtt cgcgttcgcg gtcgtcgcgt gggagtgcct gtacggcaag cgcccgttcg
     3781 cgggcatgga tcgcgagaaa cgtgcggagg ctccgcgcag cgatgttccg gtgcgcgtgc
     3841 gtcgggtgct cgagcgtggc ctcgccattg atcccgcagc acgcttcgcc gacatgccgg
     3901 cactgctcgc agcgcttcgc acgtcgtcgc gcccacgcac cgcgcaatgg atcgcggcgt
     3961 cgctcgcggg cgtcgccgtg atcggcggtg ttgcatacgc gggcgcgggc atgtatcgcg
     4021 atcgcgagcg tcatgcggcg tgcgagctcg agggtgatcg agtcgcgagt gtgctgcgcc
     4081 cggagcagca ggtcgcgatc cgcatgagct tcctcgcgac gcgatcgccg atggccgacg
     4141 gtgcgtacga gcatgcgatg agcgtgctca ctccgtatgc gaagagtctc ggcgcaaaga
     4201 ccacgcaagc atgcctgacg gccgacgagc cgatgcacgc tgcacgcgct cgctgcctgt
     4261 cggcgcgcaa gaatcgcctc gccacactgg ccgacggctt cgcgcacgcg acgaagggca
     4321 agacgacggt ctctcgcgcg gcggaaatcg cttggaacgt cgcgcacacc gatccatgca
     4381 gcgacgaaga tccggtgctg acagagcgca agcacgcacc gacggatccg gtgctcgcga
     4441 aggcgctcga cgacgtgtac gcggttcgcg acgcaggcaa atacgacaag gagctcgaag
     4501 ccgcgactgc gctgctcgcg aaagcgcgag ccgccggcga tcgcgagacc gagctcgacg
     4561 cgctgctcgc ccaggcgatg gcgcaagacg cactcgagaa acccgagcgc gcgaagacat
     4621 tcgacgaggt cgctgcactt gccgaagcac tcggtcgcga cgtcacggcc gcaggcgcct
     4681 acgcgacgat ggctgctaac gccgctcgcg atcagcagga cttcaccgcg gcacatcgct
     4741 acctcggcct cgcgcgcgcg aagctcgatc gcctcggcgg caagaacatc gcaacgcgcg
     4801 gcgacctctt catcatcgaa gccaagatct tcatggacga gtaccgctgg gccgaaggcg
     4861 aggccgccgg ccggcaaggc gtcgcgctcc tcgagcaagc actcggaccg aaccacccga
     4921 aggtcgggat cgcgacgggc acgtggagcc agctgctccg catggtcgac aagaacgacg
     4981 aagcactcgc ccagagcgaa cgcacactcg acatcttcac gaaggcgtac ggtgccgacc
     5041 atccgacgac cgcgggctcg tcgctgaacc tcgcgcagag cctcatcgat gccaagcgct
     5101 acgacgaagc tcgcgagcgc ctgctctccg ccgacaaagt cttcatgcgc acgttcgggc
     5161 cgaaccatcc cgtgcacatc tccgcatggt cgaacctcgc gaccgtcgag cagctgcaag
     5221 agcactggga cctcgcgctg tcgtacgaca ggcgcgtgct cgcgatgatg gaagcgctcg
     5281 gcggtccgga ctccgcggat gcatcgggcg ctcaccgcga catcgcaaag gtgctcgcac
     5341 tctcgaacca tccgcatgaa gcactcgccg agcagcaacg cgcggtctcg atcctcgaga
     5401 agctcggcga ggacggcgaa ggccgcatga tcggcgcgca agtcgagctc ggcgaaatgc
     5461 agctcgagct cggtaacaag agcgcggcga aggtctcgtt gcaccgcgta ctggatctag
     5521 cggcgaagca tccgaacatg gccgaccccg tcctcgacgt gaagcgagcg caagagctgc
     5581 tcgcatacgc cgaaggacgc acgccgatgc ccggctccgc caagagcgcg agcaagtagc
     5641 tacgctagcg caggtcggcg gagcggtcgt tcggcacgcc gtgctgttcg aggatcgctt
     5701 tgtgtgccgc cgtgaccttc tcggcatcga gcacgatccg cgtgccgtat gcgtgatgaa
     5761 agtccgacga gaacgacgcg cgacgcgagc cgtggaaatc cgtctcgatc acgtgaaagc
     5821 cgctcgtcgg ctgctcgagt gcgcgcacga cgtcgcggat ctcggtgatg tcgacgccgt
     5881 tgacgcgctc gacgacagcg ccttggactt ggtggtagcc gaggttgatc tcgtgcgcga
     5941 gcacctggtg gatgatgacc acctgcttgc ggcgctccga cggcatgact tcgtaataga
     6001 agttcatgta gcggtggtgc tccttcgccc attcccagtc gtgcatgtaa tcgtgcgtca
     6061 gcacggtgaa cacgagaccg ccgaacacga ggtatgtcgg gcggcgttcg ggttgcggcg
     6121 gagggacaag cgagacgaac ttcttcagcg tcacgcgaag gtgcagcggt ttgcccgcgc
     6181 gcagcacgcc gaggtcgagc tcgcgcccga cttgatgacg cgcgatcagg tgctcgaacc
     6241 gcacgcgatc gccgggacgc agtggcacgc tgccatcgca cgcgacggcg gttccctcga
     6301 tcgacgtgat gacgtcatcg acttcgagca cgccatgcgc cgacgagctg tggagaacgc
     6361 gcgagacgcg cacgccggtg tcgtcggagc gcaggcccag gaagcggcgg agcgcgtcgt
     6421 tctcgatctt ctgccagtag acgccgaggt ccgggacgcc gtcgatgcga ccgtcttcga
     6481 gatcttcgag catgtgcttg atcacgtgcg tcggcacgac gtagcccgag cgctcgagat
     6541 ccttgcgctt gtaggactga aacgcgatgc cgatcagctt ctcgtccatg aagacggggc
     6601 cgccggagtt gccgggattg attgctgcgt cggtctgcag tgcgagcaag ttgcgctgcg
     6661 agtgcgtgta cgtccgaact tcgatgcgcg agacgacgcc cgcggtgatg cacagctcgt
     6721 caccgccgac cggaaagccg tagaccgtca ccttcgcgtt gcgatgcgga aggtcgccga
     6781 atgtcaccgg gtgcgtgccc tcgaagaaca ccggatcgtc gacggtcagc agcgcggtct
     6841 ctgtatcgtg atcgacgtgg agcacgcgtg cgacgtactt cttcgagtca ccgggcttcg
     6901 acgcctgcac gaacagttga tgcgcgatga cgtgcgcgtt cgtcaggatg cgattgccgt
     6961 cgatgatgca agccgaccca ccgctcgtgt gctggtacgc cagctcccac ggcttgtagt
     7021 agttcggctc cttgacgacg gtgaacagct tgacgacaga gcgttgaagc tcgacttccc
     7081 ccatgaccgc caacattact tcttgcggac gaggcggtcc gcaggaatca cgccaggttt
     7141 cttcggagac agcgcgatcg ggtcgaggtc cgcggtaccg cacaggaaac acacgccgtt
     7201 gaggatgacc tcgtggaggc aatctccgca ggccacgcag acgccctcgc ggcgttcggt
     7261 atgcgggcag gccgccggat cgacggtcac gcgctcagct tatcgcagca attcgcgtgg
     7321 catagtccgg ccgtgccgat tcgcgtgatg ccggcggtgt ggctcgcagt ggtggtggtt
     7381 gcggtcggtt cggtcgctgc gtgcgggaat gggaagggca aggagcccgc aacgtcgccg
     7441 ccgttgacgc cgggcgttgt gccgaccgct gttgccgccg cgccgccgag ccagcctccg
     7501 tcggaccgcc tgcatcatca ctatccgatt ccggtcgatc tcacgaacgt cgaggagctc
     7561 gacctcgcgg cggatggcga gcggatcgtc gccggcggtg aacagggcgg tgtcatcgtc
     7621 gtcgagcgca cgagccgcaa ggtgttgcga cgcattgccg caccgtgggt cgacagcgtg
     7681 gccatctcgg cggacggcgc gcacgtcgtc gtggtcggcg acaagaaggc gaagctcgtc
     7741 gacgtcgcga ctggcaagga gctcgcgacg cacgccgcgc aaggcacggt ggctcgctgc
     7801 ggagcgaatg gctggctcgt cgcgcgtgac aagcaggcgg acgtcctcga cgcgcagttc
     7861 aagcaagtag cgacgttggc gacgcccgat ggcttcggca ttcacggcac cgtcgcgtgc
     7921 ggcggcggtc gcgcggttct cgtcgcgaag catgcgctcg tcgtgttcga tctcgcgagc
     7981 gccaagcagc tggtgcgcat cgacgtgccg accgacggcg acgcgaacgg gctcgtcgac
     8041 gttgtcgtcg gcacgaagta catcgcgacg atctcgaatg ccggctcggt gtggctctac
     8101 gacgtcgcgg gcaagctgct agcgacggcg ccgatcattg caggccaggc gtcgcgcctc
     8161 gcgctgtcgg cggacgagag cctgctcgcg gcaggcaacg tgatcctcgc gccgcctgat
     8221 ctcgcgattc gcaagcggat ggacagcggc ggcgttgcgg cgttcacgcc ggatgctgcg
     8281 tggctcgtga cggcgcgcaa tcacgacatc gatgtcctcg atgtggccgc gctcgacacc
     8341 gatccggtcg acgggcacta cgacacgggg agctcgctcg cgatcgatcc gtcgcaggca
     8401 tatctcgcga gcggcaacgg cggccgcgtg tttctgtggg ctctcgacaa ggcgacgcct
     8461 ggcataggaa cggggccgct gctcgcgatc ctcgacggac atgcgatcgg cacgttcgac
     8521 aacgtcagcg tcgcgtttca ccccgacggc aagcgccttg tcagcgctgg ctacgcgacc
     8581 gtggtcgtct gggatctcgc gacgcgttca ccgctcgcac gctggtcggt gcaaacagac
     8641 gagtcgtacg agagcgagct cggcgtgcgc gtcgacgacc ggcacatcgt cgtgagtggc
     8701 tcgaactacg tctacgtcta cgacgcgacg acgtacaagc agctcgcgag gctcggctcg
     8761 ttcaagaccg aggtcatgga cggcagcgtc gacaacgtag cgctgcatcc cgatggcaag
     8821 cacgtcgcgg tgacgtcaca aggcggtgcg ctcgccgtgt tcgatcgcgt cagcgggaag
     8881 cgcgtcgcgc aacgccccgc cgagaaggaa gcggcagagc gattcccgat cttcacgccc
     8941 gacggcaagc tcctcatcga gctcgcatcg acgtacggcc gaacgctgac gtggccgaag
     9001 ctcgattcgg ccgcgacgtt cgaggccccg tgggcaagcg acgccgcgtg gagcgccgac
     9061 ggcaaacgcc tcgcggtcgg ctcgtggaac gggcttggcc tctccgtgtt cgagcgcgcc
     9121 ggtgcttcgc tacggaagaa gtgggagctg cagacgccgt ggcacaccgc cgcggtcgcg
     9181 tgggacaaca agcgcggcgt catctatacg accggcgccg acgagagcat tcactcgtgg
     9241 gacgccgcga ccggcaagca cgtggcctcg tgggtcggcg cgggcggcga tggcatcgac
     9301 ccgctcgtgc tcgtcggcga cacgctcctc gtctaccaat ccgagatcaa catgctgcgg
     9361 ttcttcgacc tcgcgaagct cgagcagacc gcgcacctcg acgagctcta tctcgaagac
     9421 gacaccgagc cgcgtctcat cggcgatcgc ctcctcgtgt ggacgagccc tggcaagctc
     9481 gctcggttcc agcgcgacgg cacgacgtgg aaggcgctct ccacgtggaa tccacccgag
     9541 gactacggct gggcaggcat cggaatctcc gccgacggca agctcgcatg ggcgatccac
     9601 ggcgagaccg acgccaacga tcagcaaacg ggcgagctct acacgatcga tacgtcgacg
     9661 ctcgccgtcg tctcgaagca gcgcgcgaag aatttcggcg gtgcgcagat gcgcgtcgat
     9721 ccgtcgggta agtatgtcgc gttcatcacg ggcaacgacg aagccgacaa gctcgacgtc
     9781 tggacgctct cgcccctcaa gcgcgtccgc acgctgcaga gcgcggacgg cttccgcgac
     9841 ttcgacttcg tcggcatcga tgagatcgcg ctcgacggtg acgatccgaa gcgctggtcg
     9901 atcacgaccg gcaagtcacg cctcgattgc ggcggcggct ttcacaacac gctcggctac
     9961 agcgccgatc acaagcgcga cgtgatcatc cgcggctcct cgattcgcgt ccgcacgctc
    10021 gccgactgca aggaagacgc gttcttctac gatggcgaac gcacagccgc cgtcgacgag
    10081 aagcgcttgg tcgtctcgaa ccgcggcgca attaccgtct gggatctcgc gaagcacgag
    10141 cccgtcgcaa ttctgcgcgc gcttcgcgat ggcctctggt ccgcgcgcac cgccggccac
    10201 tacacgccgc ccgcgcacgc gccgatgccg ccggcgaaga ctccgtagcg tcagcgcagc
    10261 gcgcgcagcc gcgtcgcgag gcccgaatgg cgaagcgcac cgcgcttacc ggctgccacg
    10321 atcgccgcgc gcgatccgca caacacggca ctcgtccgcg cccgcgtgat gccggtgtac
    10381 aacagctcgc gcgtgagcag tggcaggtgc tcgtcctcgg gcagcacgag tgcgagcgcg
    10441 tcgagctcgg agccttggct cttgtggacg gtcagcgccc atgccagctc cagtcgatcg
    10501 cgcagcgcct cgatcgcgaa cggaaccagc tggccttgca cgcggaacac cgcgcgatag
    10561 cgatgtgcac cggagccttc gtcggcgcgg atgatcatcc cttggtcgcc gttgtagagg
    10621 ccgcgctggt aatcgttcgc cgtgatcatg accggctccc ccgggacgaa ctcgggacgc
    10681 cctgtcacgg tcatgcgttc gagcgcgagc tggtgcacga atgcgttgag cgcgagcgag
    10741 cccgtcggca tgccgcgcgt gacggtgagt aagcgcgctc ggccaagacg cgtccagaga
    10801 tcgtcgagct cgtgctgctg cgcgggatcg acgcggccat cgctgaagcg gaacacggtc
    10861 tcactcgcca agcgctgcgc caaaggtccg tcaaagtggt gccagagcgc ggccgcgacc
    10921 gcgtgcacgg gctcgatgga cttcgacttc gaagcaccgg ccgcgctagc tgtcgtgtcc
    10981 acccactccg cgcccgacca cttcagcgtg ccgggcgtgc ggagcgcggc gagcccgttc
    11041 ttctcggtca agcgttgcgc ttcaccggca cgcaccgccc gcgcagcctc gagcacggcc
    11101 cggccgcgcg gatcggcggc atccattcga aagctgtgct cgagggtcgc gacacaaccg
    11161 gccccgacgc catcgggctc cgcgaggtct gccaggatct gtccggcatc gatcgcgggt
    11221 agctgatgcg catcgccgat caagatgagg ggcacgctgg ccggcaatgc atcgagcagc
    11281 gcgtccatca gctcgaggtc caccatcgac gcctcgtcga cgatgagtgc gccgacgggg
    11341 agcggactct gcgcgtggtg cgcgaatcca tcgccgcgat atccgagcaa gcgatgcagc
    11401 gtctgcgcgg caggcggaga cgtcaccagg gcccgatccg catccgcggg cgacgccacc
    11461 gccgcgagct gcatgccgat catctcggtc agccggttcg cagctttgcc cgtctgcgcg
    11521 gcgagcgcga tctgtatcgg cggcacgccg agccgcgcaa acccgcgcac gatcgccgca
    11581 gcgacgagcg tcttgcccgt tccgggaccg ccggtcacga ccgcgagaga tccggacagc
    11641 gcgagctcga ccgcgcgcag ctgctccgcc gacagagtgc ccttgccagg tcgcgccacg
    11701 agatcggcga tcatgtcggc cttgggcaac gagcgaggag cggctagccg tgcggccaac
    11761 cgcttcgcga cgcggagctc gagataccgc gagcgttccg tgtaaaggca gccgtcgtcg
    11821 acgatgagcg gaagccgcgc gtcgcccgtg ccgatcacgg agttgaatga cggcgccgat
    11881 gtcaggtctt tgatgcgctt gagaatgcgg ttggtgtcga gctccaagcc cgcgacgcgc
    11941 atgatctccg tgaccagtgt ccgcaggcgc gcctttgtag gcgcaggcga ctctgccggc
    12001 attgcacccg acggcgaacg cgcacgtgaa tcgtcgaggg gcaaccggct cgatccctgg
    12061 cgctgcgcga tcatgagcgc gagcacgagg atgccgagcg cctccggatc cgagccgccg
    12121 agccagcgat ctgccgcgac gatctcctcg gcgagataca gcccctcgtc accgagatcg
    12181 cagcgccgcg cgcccacgcc gagcttgcga aagatgccat cgtgatcgcc gacgtgcgcg
    12241 aacttcgcgc gcagcgaccc acgcgggtga agactcggcg gcgtcgtgct catcgcggga
    12301 cctctgtgcg taacgaggcg agccagcccg tccagtgggc caggatctcg tcggtgatgc
    12361 gaagcggaac gacgatgtcg tggcgcacga atgcgaacag cagaccggcg agccggcggt
    12421 tgccgcgcat gcgatcggcg gcgaccgcgt acaggcgcgc ttgcacgcca tacttctcgt
    12481 gcatgcggtc ggtcgcggcg gacgcgaggt catcgccgcc gaggagatcg ctcttgtagt
    12541 cgagcaccca gagctcgtcg ttccacgcga cgatggcgtc gatgaagcct ttgacgaggc
    12601 cgcgcggcgg cgcgttgggc ggtgcatcgg gggcggacgg catcgggtac gtgaactcga
    12661 gctcgcgagc gatcgcggtg gcctcgacga gcggcggcag ctcggtgccg tcggtgagcg
    12721 cgagctggcg cgtgagcgtg gtgtggacga tgcgcgccgc gtgcggcaag cattgctcgg
    12781 cgatgccgtg ctcgcgcgcc tgatcgacga ggagtcgttc gacgaccggg tttgcgcgcc
    12841 acgcgtcgac gtccttggcg gcgcgggccg tggcgagatc ggcgtgctcg aagatggcgt
    12901 ggaggagcag gcctgcgtct gcgccgggcg gaagatcgtc ggggccgagc gaggcgcgtg
    12961 cttcggcgtt gcgatcgtcg acgtcgaact cggctggatc gatcgcgtgt ggaagctcgc
    13021 ccggctcgag cgcggcggcc tcgagatcgt gggcgaggcg cgtgtacgac agcatcgcga
    13081 ggccgaggcg ggcgggcgcg agcggggcga gcggtgtgat cgtgggcggg gccgggacgt
    13141 cgaacgtggc gagcgcgtcg gcgggagctt ggggatcggg cgctacgccg acgccgacgt
    13201 gcaccgtctc gacgagcgga tgattgatct tgagcaggtg cgtcgcgcac cgctgcaccg
    13261 gcacgtacat cgccgtcttg tcgggcggct tcccgccgta gagcgggaca tagaggcgaa
    13321 tttgcgcgcg cgtgagcgcg acgtacgcga gccgctgatt ctcggcgtcg gcttccatct
    13381 cgagctgctg cttgatggcg tcgtcgttcg tgtcgacgtg cagcgtgcgg ccggccccat
    13441 cgcgcagcac gccaaccttc gatttgggac cgccggtcgc gcagccgaac aagaagacgt
    13501 acggcgcttc gagacctttc gccttgtgga tagtgaggat gcgaatcgcg tcggcatcgg
    13561 tttcggcgcg ctggacatcg cgatcgtcgg ctagctcttc gtgctcgttg atccagcggc
    13621 gcagccgcag cgtgagctcg tgcagatcac agcgcgagcg cgcgacttcc tcgagcagca
    13681 gctcgatgag gtgccacgtg ttgaccagcg cgcgttcgcc gccgccaagg acgagcgcgc
    13741 gttccgcgaa gtgcgaatcc tcgatgacgc ggcggaacag ctgctcgtac tcgcgacggc
    13801 ccgcgagcgc cgcccaatcg aacaggcggg cgatgagtgg gtgatgatcg gatgcgtcga
    13861 cgacgcgcat cagctcggcc cacgggacgt cgaagaaccg tgtgtgaaga gcacgcatgc
    13921 gcgcagagcg gtcgcgcggt gccgcaatcg cttcgagcag cgcggcaagc tcggccgctt
    13981 cccgcgtctc gaacagcttg tcgctctcga ccagcgcgca ggcgagaccg cgtgaccgca
    14041 gcacgctggc gatctgcagc gactctttgc cggtgcgcgt gagcaccatg cagtgcccga
    14101 gggtgaacgg cggcgccttg ccgcggctct gccacgcgtg cggtcgatcc cgcaggcgtt
    14161 cgatctccga cgagatcgcg agcgcgagag catcgcggtc cggatcgcgt tcacggctgc
    14221 cgccgccttc gagcgagaag atcgtcagcg gcgggcgcga gccttcgcat gtgacgtcac
    14281 ccgacgcctt gaccgggtgg tcgtacgtga ttgtcttgtc catcagcgga ccgagcgggg
    14341 gaccggcgag gagtgcgttg acggcttcga cgagctcggc ggttgaacgt cggttgacgt
    14401 cgaggtccac ttgtgtggcg ccattccgaa gcagctcgtg cttcgcgtcg aggtacgtcg
    14461 cgacatcggc gccgcggaac ccgtagatcg cttgcttcgg atcaccgacg atcgtcaggc
    14521 ccttcgcgtc cggatgcatc cagacggtgc ggaagatgtc ccactgaacg gggtcggtat
    14581 cctggaactc gtcgatcatc acccacggca tgcgagtccg cagccgcgtc gcgagctcgc
    14641 tgccgcgcgg gccatgcagc gcttcgcgga cgagatcgag catgtcgtcg tagtcgaaca
    14701 tgccgtgctc ggccttgtcg gtgccgatgc gcttgacgac atgggggagc agctcggatg
    14761 cgatcgcctc tccgagtgac atcaccttca tcgattcgat cagcgtgttc gcgaccggcc
    14821 ccttcgcgta cttgacgatc ttctcggcga cgtcacgaag ctcgtcgcat gcggcaagcg
    14881 caacggcggg atcgtcgccg cgcgctcggt cgaggacgcg gccggcttcg tcgacccaat
    14941 ccgcggcgta gcgctgaccg cggccccagc tcgtcgtgtg caggaacctc tcgcgttcgt
    15001 cggccgtgcc gagcatggcg cgcagttggc cgagcacctc gactgcgcgc tggggatcga
    15061 ggctgcgccg cggacgagca tcggcgcgcg cgcacttcag caggaggtcg cgcaggttgt
    15121 cgacggtctt gccccactct agataagccg caagcagctc cttgtccggc gagtgggtcg
    15181 cgaagcgctc gcgcaacagc gcgttgaatg cggtgtcgaa cgcgacttcg tctgcgacct
    15241 gggtctgatc gaacagccgg cgcgccgcga acgcgtcttc gatcaggatg cgatggcaga
    15301 aggcgtgaat cgtgaagatc ggcgcgtgat cgaacgcagt gacggcggcg cgcagccgcg
    15361 cccgcgcctc gtcgtcgatg ttccacgtcg acggcgcgac ctgatcgata tcggcgcgcg
    15421 ccattcgatc gagtaagtcc cggatgcgca tgcgcagctc ggccaccgct ttgtcggtga
    15481 acgtcacgag caagatctgc gacagctcgg ctcccgcgag caccagatcg acgacgcgat
    15541 gctcgaggaa gaacgtcttg cccgtaccgg cggacgcctc gaccacgacc agccgatcgc
    15601 tcgccggcgg caacgaatcg ggacgcatga cgcgggtcat cgcgcacctc gacgagctga
    15661 ggtcatcgtg agcgataggc gcgtcatcac tcgctgcctc cgagcgagtg atcgcccgtc
    15721 atccgcacga cgagtgggcc gaggcgacgc ttcgcgatcg agcccggatc gggcggagcg
    15781 acgagcccat cgattcggtc gatcggcccg aatccaagaa gactcgtgat cgcgtcgccg
    15841 tagatgcggc cgggcggttt gccggcgtag ccgttcacca ggtgatcgaa tggcagcaga
    15901 tagccgtgcg attgatcgag cagctcaccg acgagggacg tcaagaactc gcgcgcatcc
    15961 gcctgcgacc acggtgcatg ctggacggag cgcacggtcc cctctggatc gagcagcagg
    16021 tgctcgtgcg agcccgtcga gaggcccgcg gctgcgagca ctacatgatc gagtgcgccc
    16081 cgcaggtgat ggcgcgagcg cttgtcggct ttgccgatca gcggaatcac ggacgcatag
    16141 ccggtgcctc gacgtacgag cagctcggtc tgaccgacga gacgcacggt gcgtccactc
    16201 gcgagcgata gctcgagcgc aggcatgagc tcggccattg gcgacgacga acggccgaac
//
LOCUS       DATLBZ010000320         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_361096, whole genome shotgun sequence.
ACCESSION   DATLBZ010000320 DATLBZ010000000
VERSION     DATLBZ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361096"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VMZ53_16855"
     CDS             <1..250
                     /locus_tag="VMZ53_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUS30181.1"
                     /translation="PAPRTLRPRDGVAYAINWMAVEGFRNVPIVDDAGVLQGVLTVWD
                     VMRHLGDVFDDIDATPKSTPPKSDISEVISMVDLGGGG"
     gene            274..714
                     /locus_tag="VMZ53_16860"
     CDS             274..714
                     /locus_tag="VMZ53_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUS30182.1"
                     /translation="MRTLAIAQVMDLEKRPHATVAVDAPMWKVVDEMKAKGRGAVIVE
                     DEGALVGIFTERDLVGRLDHTDIMWSHVIVKDVMTPHPTVVRPEDSLAEALRLLVQGR
                     RRHLPVVDPKGKVTGLVSIRDILSFIASKFPEDMINLPPNPDHE"
     gene            complement(716..2236)
                     /locus_tag="VMZ53_16865"
     CDS             complement(716..2236)
                     /locus_tag="VMZ53_16865"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30183.1"
                     /translation="MDALAEGTVVGGRYVVNAPIGDGAMGTVYRAKHARVGRDFAIKV
                     LHRSLMKDPKAVKRFSREAELAGRLRHQNVVSVVDVGLTYNGLRYMAMELAPGTSLGH
                     ILLDGPLSETRTLDIAKQLCAGLAHAHELGLIHRDFKPDNVLVEHAKGGREVARIVDF
                     GVAILREDVGDSDGDRLTTKGLVVGTPHYMAPEQARGVAFDHRVDIYALGLIMYEMLS
                     GVLPFEGTGVEVAHAHVSSPPPPLRERAPDCHIDADLEAIVMRMLAKDPSDRYDNADE
                     VLQALQGLERRRSSPRNRIVTDVVAPISRAHSVSDVAALIVTEQVEPLALRPTRLHPT
                     PLASRSRTGNRRVRRVLAASAVCVALSALLVAGRARNRAEAGSAASIDVASNESIGAG
                     KDERIHRETLPPGPQILEQKNQRAIVEVPLPPTAQQTLPTSNVVTPSAQEVARLYATV
                     GRELHTLEAAHGMNATIDLWPRYRWIRINDAMATAEKRMQSTVMLQRLQRDIRSAL"
BASE COUNT          429 a          805 c          846 g          453 t
ORIGIN      
        1 acccgcgccg cgcacgctgc gtcctcgcga tggcgttgcg tacgcgatca actggatggc
       61 ggtcgaaggc ttccgcaacg tgccgatcgt cgatgacgcg ggcgtgttgc aaggcgtgct
      121 caccgtctgg gacgtgatgc gtcacctcgg cgacgtgttc gacgacatcg atgcgacgcc
      181 gaaatccacg ccaccgaagt cggacatctc cgaagtcatc agcatggtcg acctgggtgg
      241 tggcggatga ccggcgcaaa tgtttcgcaa gggatgcgca cgctcgcgat cgcgcaagtg
      301 atggatctcg agaagcggcc gcacgcgacc gtcgccgtcg acgcgccgat gtggaaggtc
      361 gtcgacgaga tgaaggccaa aggtcgcggc gccgttatcg tcgaggatga gggcgcgctc
      421 gttggcatct tcacggagcg cgacctcgtc ggccggctcg atcacacaga catcatgtgg
      481 tcccacgtga tcgtcaaaga cgtgatgacg ccgcacccga cggtcgtgcg ccccgaagac
      541 tcgctcgcag aagcgctgcg cctgcttgtc caaggacgtc gccgtcacct tcccgtcgtc
      601 gatcccaaag gcaaagtcac gggcctcgtc tcgatccgcg acatcctctc gttcatcgcg
      661 tcgaagttcc cggaggacat gatcaacctc ccgccgaacc ccgaccacga ataggtcaga
      721 gagcggagcg gatgtcgcgc tgcaagcgct gcagcatcac cgtgctctgc atacgcttct
      781 cggccgtcgc catcgcatcg ttgatgcgga tccagcgata gcgcggccat agatcgatcg
      841 tcgcgttcat gccgtgcgcg gcttcgagcg tatgcagctc gcgaccgacg gtggcgtaga
      901 gccgcgcgac ttcttgggcg gatggcgtca cgacattcga cgtcggcagc gtctgctgcg
      961 cggtgggcgg cagcggcact tcgacgatcg cgcgctggtt cttctgctcg aggatctgcg
     1021 gcccgggtgg cagcgtctcg cgatgaatgc gctcgtcttt gcccgcgcca atggactcgt
     1081 tgctcgcgac atcgatcgat gcagcagagc cggcttccgc gcggttgcgc gcgcgacccg
     1141 cgacgagcag tgcggacaga gcgacgcata ctgctgacgc agcgaggact cgacggacgc
     1201 gacggttgcc ggtgcggctg cgcgaggcga gcggcgttgg atgcaggcgc gtggggcgga
     1261 gcgcgagcgg ctcgacttgc tcggtgacga tgagcgcggc gacatcgctg accgagtgcg
     1321 cgcgagagat cggcgcgacg acgtcagtga cgatgcgatt gcgcgggctc gatcggcgtc
     1381 gctcgaggcc ttgtagcgct tgcagcactt cgtccgcgtt gtcgtagcga tcgcttgggt
     1441 ccttggcgag catccgcatc acgatcgctt cgagatccgc atcgatgtga cagtcgggtg
     1501 cacgttcgcg gagcggtggt ggcggcgacg atacgtgcgc gtgcgcaacc tcgacgccgg
     1561 tgccttcgaa cggcagcacg ccgctgagca tctcgtacat gatgaggccg agcgcataga
     1621 tgtcgacgcg gtgatcgaac gcgacgccac gcgcctgctc gggcgccatg tagtgcggcg
     1681 ttccgacgac gagccccttc gtggtgaggc ggtcaccatc gctgtcacct acgtcttcgc
     1741 gcaggatggc gacgccgaaa tcgacgatgc gcgcgacctc gcggccgcct ttcgcatgct
     1801 cgaccagcac gttgtccggc ttgaagtcgc ggtgaatgag gccgagctcg tgtgcgtgcg
     1861 cgagacctgc gcagagctgc ttcgcgatat cgagcgtgcg agtttcggag agcgggccgt
     1921 cgaggaggat gtggcccagg ctcgtgccgg gcgcgagctc catcgccata tagcgaaggc
     1981 cgttgtacgt gagcccgacg tcgacgaccg agacgacgtt ttggtgacga aggcgtccgg
     2041 cgagctcggc ttcgcgagag aagcgtttga ccgctttggg atccttcatc aagctgcgat
     2101 gcaggacctt gatcgcgaag tcgcggccaa ctcgcgcatg cttcgcgcga tagacggttc
     2161 ccatcgcgcc atcaccgatc ggcgcgttca cgacatagcg gccgcccacg accgtccctt
     2221 cagccagcgc gtccatcacc cagccagcag cgtaatattc gaccttcttt tcgtacgctc
     2281 cctaactgtg ccctcgcgca cggtagcgtc gaagcggccg tatgccctcg gaattgcttc
     2341 caggcgcaac gcgttagagg tatgaggtcc ccatgggcac gacacgcttc gaaggctgtc
     2401 tcaccgcgct cgtcactcca ttcgcgaacg gcaaggtcga tttcgacggc ctcgcgaagc
     2461 tggtcgactg gcagatcgag aacggcgtcg acggcatcgt ctcggtcggt acgaccggcg
     2521 agtccgccac gct
//
LOCUS       DATLBZ010000321        12289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10616566, whole genome shotgun sequence.
ACCESSION   DATLBZ010000321 DATLBZ010000000
VERSION     DATLBZ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12289
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10616566"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VMZ53_16870"
     CDS             <1..1005
                     /locus_tag="VMZ53_16870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HUS30184.1"
                     /translation="TWNPGAQRIKGYAADEIIGRHFSTFYPKASIADGLCERELAGAV
                     RDGRFEDEGWRVRKDGSLFWANVIITALYDGAGKHIGFGKITRDLTERRNAENDRVRL
                     AQAEEALRLRDEFLSIAAHELRTPLGALQLQIDSLCAQSQTLDDRLRAKANRAARNAE
                     RLGELIAALLDVSRIATGRLTIAPRRCDLDNIVLDVLERMQELTAQAGCTVTTNIERG
                     ITGQWDPLRIGQVIANLISNSSRYAAKSPIDISVVREGDHAIVRVEDRGPGIPEDALE
                     RIFGRFERAASPRSYGGLGIGLYVARQIVLAHGGTIEARNRSAGGALLEIRLPIQDSV
                     "
     gene            1009..1383
                     /locus_tag="VMZ53_16875"
     CDS             1009..1383
                     /locus_tag="VMZ53_16875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS30185.1"
                     /translation="MAVPNRVLVVDDDVELRETFVDVLAENGFEAIGAADGIDALLQL
                     RDPEDRWALVLLDLMMPNMDGRTFRREQMSDAALASIPVVILSAATDVADVARGLDAV
                     AAMTKPVRLVDLIAIVSKYCPR"
     gene            1479..1862
                     /locus_tag="VMZ53_16880"
     CDS             1479..1862
                     /locus_tag="VMZ53_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS30186.1"
                     /translation="MKILVVDDNVELAEMIAAHLRLYAHDVVVALDGASALRAVRREQ
                     VDAILLDVGLPDVSGYEVARTLRDGILQRKAVIALFTGDGEPSFDRAKAVGVDLVLRK
                     PLAPDELRILLGFLHDRRQQRVVLT"
     gene            complement(1855..2757)
                     /locus_tag="VMZ53_16885"
     CDS             complement(1855..2757)
                     /locus_tag="VMZ53_16885"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUS30187.1"
                     /translation="MKYVEPRMLNYNHLHYFHIVASEGTVAAAAQRLGLTQPTVSEQI
                     RSLERELGVTLFERSTTGLTLTEAGRAAFEQTSVMFRASERLVEVIHDDRRAIPASLR
                     VGISTAVSRSTATDFLLPLLTLENCVPVIRTGDWLQLMRDARAGELDLVLMETEPSNA
                     LRRGLDVALVGRTQLVAVAAPSVKPSETWDNVALIHYRVSAPVRWEVEAFLQARHLRP
                     KIAAEADDAAFLVEAAERSGCIAIVPRPVVRDSLSAGRLVVVAEIEPSQAAVYAAYQE
                     TATVDLARRAVQILIAHLRMAEIK"
     gene            complement(2906..6265)
                     /locus_tag="VMZ53_16890"
     CDS             complement(2906..6265)
                     /locus_tag="VMZ53_16890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30188.1"
                     /translation="MSADPADRTATLQPTMPSKAPRHEEATGDEAQLPIDSPDRYEHV
                     GEHARGGLGRVVRAVDKRLGRTVAVKELLRQDESHEARFVREALITARLEHPGIVPVH
                     EAGRWPNGAPYYVMKLVEGRTLKELFAEKKTLRDRLGLLAHVIAIADAVGYAHSEGVI
                     HRDLKPSNVIVGEFGETIVVDWGLARDTRGHLPEPTTELLETRAKGSGASTISGKVVG
                     TPAYMAPEQARGELVDERADVYAIGAVLYELLAGKPAYTDPTPQGVLDRVLAGPPAPL
                     ATSAPGASQELCDIVGKAMARVPADRYANASALAEDLRRFQTGQLVSAHAYTPWQLVR
                     KKLAQHRGVVVVAIASAIALGAVGVESFRTVVAERDIARGERARAEQEKQSAEQRKRD
                     LLYVQAETSLRKDPTAALAWLKLHPVSDRERAQVVDVIDEALALGAARHVFRPGDWVF
                     DAMFTPDGRTLVAAVRDGKLRTYDVASGELHVLGQSKAGIEALAISPDGKTVITGGSV
                     GEITAWSLTDGTKKVLVDSGRPVMAMRFAPSGDKLLVDQDGLTVMVGLDGVVTKLGPD
                     SSVLLAVAANDWSKRVAKVSPNQAIIVDENGQPQRVLASLDKLIQFIALSPNGETVIV
                     HDVDTIYAVPFAGGPPKKLVTFMTKLNMTVWSPDGQTVALVGIHNDLTLVSVKTGEVT
                     ELRGHTDSIYTAEFSRDGKTLLTASDDGTARVWNIADRSSRVLTGHDDDVYRARFSPD
                     EKTVATASLDGSIRVWPIDRSGARAFTEGAQDLDMDLVGDLVTIRTIRGVARWDITKG
                     TREEIFERQGLGIGVPSPNNQYLVAQDADWTLQLYDRTGAHRPLRGHKGYISHVEWSQ
                     DSKYLFSASFDGTLRRWDAVTGESTLLVEGDVPVRGFAVARDGRVAAQVGEAAVMIKA
                     DGSAETLGTGSGWCTTKAEFDRAKDRLLIQRCDRGLLLVDGKTAVNLPTDGYTVVRLA
                     VSADGQRIAAAMSDRTVRIWDTAGKVIAQLRGHTDLVLDVAFSPDGTRLASSAYDKTI
                     RVWELATGRYRVLRGHARAVGNVEWHGQNELVSSSWDGTIRVWPVPTLDAPTQEEITK
                     RLDDATTAAIDANNRATTLQASPSPSGAQRL"
     gene            6373..7542
                     /locus_tag="VMZ53_16895"
     CDS             6373..7542
                     /locus_tag="VMZ53_16895"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HUS30189.1"
                     /translation="MTGKVLLAAIGVALVSRPVAADPKPDYAAIAERVVGTSANVKEG
                     EIVWISVGPQDLAFADELAAAVRKRGAFPIVTYSSEAMTKRVFATMPDKYDAQAPKGD
                     LELTKIAATHINISSVRDPSIWLDGISPERRRKIFAAGASAQSLAVKRHVRLVELDNG
                     LLPSATRAKETGLSEAELTKQFWDGVGADYTAVSAQCKKLTDALAKGGEVHITHPNGT
                     DVKMKLKPKKTAASDGILTDAKVKAGGPGVQVWLPAGEVYFVPVISSVEGKVVDDRMA
                     IFGKEILGHSFDLKAGKVANRATKSGWDAIKADYDAAGPGKDEFGMVDLGCNSAVKNT
                     AKLETWMAAGTVSITTGNNEYAGGTNKEPLGMAHMLSGATLTLDGKALIENGALK"
     gene            complement(7547..7906)
                     /locus_tag="VMZ53_16900"
     CDS             complement(7547..7906)
                     /locus_tag="VMZ53_16900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS30190.1"
                     /translation="MASAVSPIVLVVEDEEDARETLRELLELRGYNVRTAADGQQALD
                     AIDSAELPICIVLLDLFMPNVDGWAVYETLQTRGQLESLRVLITTSAPHRAPAGSSVM
                     GKPLDLSKLFELLQSCC"
     gene            complement(7921..10206)
                     /locus_tag="VMZ53_16905"
     CDS             complement(7921..10206)
                     /locus_tag="VMZ53_16905"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS30191.1"
                     /translation="MNEPLHSTIEAVGGVVALAMATLLLRGSALEDRPYLEWVAASLI
                     AQGVLDIVHAITPPGATFYWTRTVPTVLGALLFALVWLPRRRVPILPAVVLGIAVTLG
                     ILLVAAPNAWPSAFERGFNYRPWAQHINQVAGAGFVAAAVFMLREQRRRGSFIDAVFA
                     SYAVLLALAALMFGFSQPWDGTWWAFHVLRFTACVVSLGTVVDLLQRRQRLRLEQLNS
                     DLGAAQQEAERERETLRAVIMQAPIPMALYAKEELRVVLANDAYIAVTGDRDVRGKPL
                     LEAVPELRHRPTFHVIQDALATGEVRTLPEYVAPLRDADGVLRDRYFTATYQPMNIDD
                     ETRGVIVVGMDITEQVQARRVAEQFGQRMSVLVQASRAFTQAGLDRAAIFEAVVQQVV
                     EHSFDSAALHVIDRAAKQVRLVALRHRDPDVQAGMQAILSEPFALGEGIAGRVAQTGI
                     SVLTPAIDRAALAGTMRPEHVRFIEEHPVKGFVTVAVRARGEILGTLSVTRFVDEPRL
                     GEDDKVLLEDLAGRAGLAIMAADQFTSLQQSLRFADQFVGMLGHDLRNPLNAITVGAA
                     LVREKGGESVKVNAERILRSAKRMSAMVSQLLDLTRVRLGEGITLAREQINLAETLDT
                     IIGELELLYPGREIRRKVEGNTTGMWDGSRMAQVIANLLGNALQHGDSAAPVEARIEG
                     TSTGVRITIHNAGEPIQPDIMPVLFDAYRRTDARSHRSEGLGLGLSISQQIVMAHGGT
                     IAARSTKTEGTTFTIELPRPP"
     gene            10420..11256
                     /locus_tag="VMZ53_16910"
     CDS             10420..11256
                     /locus_tag="VMZ53_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30192.1"
                     /translation="MQRAVLATIIVTSACGPSGRDGNGDLPDARPSGDGQVTLPASRV
                     YAHSGSKLYQVDSQPPHTPVEIGTMGGIGTQSLTDLAIDKTDRMVGITLDKLYTINPT
                     TGAATLVTDLSTTARGFTSLSFVPADINNPSSADILVSANDQGDVYSIDAMNGNATKI
                     GSYGSVSAGKVISSGDLIGVQGLGIYATVDVGTQPNDYLARIDPTTWKATPIGTASTG
                     YDNIFGLAFWEGKIYGFVSAGTDAGKIITIDPTTGVGTQVQSGAVRWYGAGVATDAPI
                     IQ"
     gene            complement(11246..11641)
                     /locus_tag="VMZ53_16915"
     CDS             complement(11246..11641)
                     /locus_tag="VMZ53_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30193.1"
                     /translation="MSRQLKIIVGAIVAVVLGSVAVAVFVMRGQSSESSSETTVRIEV
                     RALPQMTITKDGKTIGKTPLSFVVPKSTKPISLDATWTEQRIYKKGGEQMVPREAHKD
                     VVPDRTQAVDFSRRDGKPVPPPMVEPATE"
     gene            11677..>12289
                     /locus_tag="VMZ53_16920"
     CDS             11677..>12289
                     /locus_tag="VMZ53_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HUS30194.1"
                     /translation="MTLPIRRVAVLGAGVMGAGIAAHCANAGLPVLLLDIVKPDLDKL
                     KKAKPAAFMHNRNAQLIETGLFDTHLEKVKDCDIIIEAIIERLDIKQQMFEKLEKIAP
                     NAIVASNTSGLRIADMLQGRSEAFRKNFVVTHFFNPPRYMKLLELVAGPDTSAEAKQR
                     VEAFGRDVLGKGIVWGKDTPNFVGNRIGVQSMLTTIHLMIEKGL"
BASE COUNT         2205 a         3968 c         4027 g         2089 t
ORIGIN      
        1 acatggaatc ccggcgcgca gcgcatcaag ggctacgccg cggacgagat catcggccgg
       61 cacttctcga cgttctatcc gaaagcatcg atcgccgatg ggctgtgcga acgcgagctc
      121 gccggcgcgg tgcgtgacgg acgcttcgaa gacgaaggct ggcgagtccg caaagacggc
      181 agcctgttct gggcgaacgt gatcatcact gcgctctacg atggggccgg caagcacatt
      241 ggctttggca agatcacgcg cgatctcacg gagcgccgca acgccgagaa cgatcgcgtg
      301 cgcctcgcac aggccgagga ggcgctgcgt ttacgcgacg agtttctgtc gattgcagcg
      361 cacgagctac gcacgccgct cggtgcactt cagctccaga tcgatagtct gtgcgcgcag
      421 agtcagactc tcgacgaccg gctgcgcgcg aaggcgaatc gtgccgcgcg caatgcggag
      481 cggctgggtg agctcatcgc tgcgctgctc gatgtctcgc gcatcgcgac gggacgcctg
      541 acgatcgcgc cgcggcgctg cgacctcgac aacatcgtgc tcgatgtcct cgagcggatg
      601 caggagctca ctgcgcaggc aggttgcacg gtgaccacga acatcgagcg cggcatcacg
      661 gggcagtggg atccgctgcg tatcggtcag gtcatcgcaa acctcatctc gaactcgagc
      721 aggtacgcgg ctaaatcgcc gatcgacatc agcgtcgtgc gcgagggcga tcacgccatc
      781 gttcgcgtcg aggatcgcgg tcctggcatt ccggaggatg cgctagaacg catcttcggg
      841 cgcttcgagc gtgccgcttc gccgcggagc tatggcggct taggaatcgg tctctacgtc
      901 gcacgtcaga tcgtcctcgc ccatggagga acgatcgaag cacgaaaccg ctctgcagga
      961 ggcgctttgc tcgaaatccg acttccgatt caggacagcg tttaaaccgt ggccgtaccc
     1021 aatcgagtac tggtcgtcga cgatgacgtc gagctccgcg agacgtttgt agatgttctc
     1081 gcggagaacg gattcgaggc gatcggcgct gccgacggga tcgatgcgtt gttgcagctt
     1141 cgcgatcccg aagatcgatg ggcgctcgtc ctgctcgacc tgatgatgcc gaatatggat
     1201 ggacggacat ttcgtcggga gcagatgtcc gacgcggcac tcgcatccat tcctgtcgtc
     1261 attctctccg cggcgacgga tgtcgcggac gtggcgaggg gcctcgatgc cgtggccgcg
     1321 atgacgaagc cggtgcgcct cgttgacctg atcgcgatcg tctcgaagta ctgcccgcgc
     1381 tagtgcgcga ctgcgccacg gtgagaccac cgagcgcact aaacagctcg tacgtggtgt
     1441 tttggacgct ttggtgtatt tgttgaggac acgacgccat gaagatcctc gtcgtcgatg
     1501 acaacgtcga gctcgccgaa atgatagcgg cgcatcttcg tctgtacgcg catgacgtcg
     1561 tcgtcgcgct cgatggtgca tccgcgctgc gggctgtccg gcgcgaacaa gtcgacgcga
     1621 tcctgctcga cgtgggactg ccagacgtga gtggctacga agtcgcgcgc acgttgcgcg
     1681 atggaattct ccagcgcaag gccgtgatcg cgctgttcac cggcgatggg gaaccatcgt
     1741 ttgaccgcgc gaaggccgtc ggcgtcgacc tcgtgctgcg caagccgcta gcgccagacg
     1801 agctgcgcat cttgctcggc tttctgcacg accgccgtca gcagcgcgtc gtactcactt
     1861 gatctcggcc atcctaaggt gcgcgatgag gatctggacg gctcgccgcg cgagatcgac
     1921 cgtggctgtt tcctgatacg cggcgtagac ggcagcttgg gagggctcga tctcggcgac
     1981 gacgacgaga cgaccagcgc tgagcgaatc tcgaacgact ggacgaggaa cgatcgcgat
     2041 acagccagac ctctcggccg cctcgacgag aaacgcggcg tcgtctgctt cggcggcgat
     2101 cttcggacga aggtggcgcg cttgcaggaa ggcctcgacc tcccagcgaa cgggtgccga
     2161 gacgcgatag tgaatgagcg cgacgttgtc ccacgtctcg ctcggcttaa cgctcggagc
     2221 cgcgacggcg acaagttgcg tgcggccgac gagcgcgacg tccaaaccgc gtcgcagtgc
     2281 gttgcttggc tccgtctcca tgagcaccag gtcaagctcg ccagcccggg catcacgcat
     2341 gagctgaagc caatcgccgg tgcggatcac ggggacgcag ttctcgagcg tgagcaacgg
     2401 gagcagaaag tcggtagcgg tcgaacgact caccgcagtg ctaatgccga cacgtagcga
     2461 cgcggggatt gcgcgccgat catcatggat gacctcgacg aggcgctcgc tcgcgcggaa
     2521 catcaccgat gtctgctcga acgcggcacg gcccgcttcg gtcaacgtga ggccggttgt
     2581 cgagcgctcg aacagcgtca ccccgagctc gcgctcgaga ctgcggatct gctcgctgac
     2641 ggtcggctgc gtaaggccaa gacgctgagc agctgccgcg accgtgcctt cactcgccac
     2701 gatgtggaag tagtggaggt ggttgtaatt gagcatccgt ggctctacgt acttcaaggg
     2761 acgagccaca tcgtcgagga aacactcgtg ttaccgccgc tactcgttca ctcgaacatc
     2821 ggaacctagt ccgttaccgg cgaagcacat gcccgaaact gcactgggcg tacgtctgcc
     2881 cagcgagcga acccgctcac aaggttcaga ggcgctgcgc gccgctcggg gacgggcttg
     2941 cctgcagcgt cgtcgcgcgg ttgttcgcat cgatcgctgc tgtcgtcgcg tcgtcgagcc
     3001 gcttcgtgat ctcttcttgt gtcggtgcgt cgagggtcgg cacgggccac acgcgaatgg
     3061 tgccgtccca cgaggagctc acgagctcgt tctggccatg ccattcgacg ttgccgacgg
     3121 cgcgagcgtg gccgcggaga acgcgatagc ggccggtcgc gagctcccat acgcgaatcg
     3181 tcttgtcgta tgccgacgag gcgagccgcg tgccgtccgg ggagaacgcg acgtcgagca
     3241 cgaggtccgt gtggccacgc agttgggcga tgactttgcc tgcggtgtcc cagatgcgaa
     3301 ccgtgcgatc gctcatcgcc gccgcaatac gttggccatc agccgagacg gcgaggcgca
     3361 cgaccgtgta gccgtcggtc ggcagattca ccgcggtctt gccatcgacg aggagcaagc
     3421 cgcgatcgca gcgctggatc agcaagcgat ccttcgcgcg atcgaactct gctttcgtcg
     3481 tgcaccagcc gctgcccgtg ccgagtgttt ccgcgctgcc gtccgctttg atcatgaccg
     3541 ctgcctcgcc aacctgcgcg gcaacgcgcc catcgcgcgc aacggcaaag ccgcgcaccg
     3601 gcacatcgcc ctcgacgagc aacgtgctct cgccggtgac cgcatcccag cggcgcagcg
     3661 tgccgtcgaa cgaggcgctg aacagatact tgctgtcctg gctccactcg acgtgactga
     3721 tgtagccctt gtgaccgcgc agaggccggt gcgcgcccgt ccgatcgtag agctgaagag
     3781 tccagtccgc gtcttgcgcc acgagatact ggttgttcgg cgacggcacg ccgatgccga
     3841 gcccttgcct ctcgaagatt tcctcgcgcg tgcccttcgt gatgtcccag cgcgcgacgc
     3901 ctcggatcgt ccggatcgtg acgaggtcac cgacgagatc catatcgagg tcctgcgcac
     3961 cttcggtgaa cgcgcgcgcg ccgctgcgat cgatgggcca cacgcgaatg ctgccgtcga
     4021 ggctcgccgt tgcgaccgtc ttctcgtcgg gggagaaccg cgcgcgatag acgtcgtcgt
     4081 cgtgaccggt cagcacgcgc gaggaccgat cggcgatgtt ccacacgcgc gccgtgccgt
     4141 cgtcgctcgc ggtcagcagc gtcttgccgt cgcgcgaaaa ctcggccgtg tagatcgagt
     4201 ctgtgtgacc gcggagctct gtgacctcgc cggtcttcac gctcacgagc gtcaagtcat
     4261 tgtgaatgcc gacgagcgcg acggtctggc catcgggcga ccacaccgtc atgttgagct
     4321 tcgtcatgaa cgtgacgagc ttcttcggtg gaccgcctgc aaacggcacc gcgtagatcg
     4381 tatcgacgtc gtggacgatg acggtctcgc catttggcga cagcgcgatg aactggatca
     4441 gcttgtcgag ggatgcgagc acgcgctgcg gctggccatt ctcgtcgacg atgatcgcct
     4501 ggttcggcga gaccttcgcg actcgcttcg accaatcgtt cgcggcaacc gcgaggagca
     4561 cgctcgagtc aggtccgagc ttcgtgacga caccgtcgag accgaccatc acggtgaggc
     4621 cgtcttgatc gacgagcagc ttgtctccgc tcggcgcgaa tcgcatcgcc atcaccgggc
     4681 gccccgaatc gacgagcact ttcttcgtgc cgtccgtgag cgaccacgcc gtgatctcac
     4741 cgacggagcc gccggtaatg acggtcttgc catcgggcga gatcgcgagt gcttcgattc
     4801 ccgccttcga ttggccgagc acgtgcagct cgccgcttgc aacgtcatac gtgcgcagct
     4861 tgccgtctcg cacggcggcg acgagcgtcc tgccgtcggg cgtgaacatc gcgtcgaaca
     4921 cccaatcgcc cgggcgaaat acgtggcgcg cggcaccgag ggcgagcgct tcatcgatca
     4981 cgtcgacgac ttgcgcgcgc tcgcgatcgc tcacgggatg aagcttgagc catgcgagcg
     5041 cagcggtcgg atccttgcgc agcgacgtct cggcctgcac gtacaacagg tcgcgcttgc
     5101 gctgctcggc tgattgcttc tcctgctccg cgcgtgcgcg ctcgccccgg gcgatatcgc
     5161 gttcggcgac gacggtgcgg aacgattcga cgccgacggc acccagcgca atcgcgctcg
     5221 cgatcgcgac aacgaccacg ccacgatgct gcgcgagctt cttgcgcacg agctgccacg
     5281 gcgtatacgc gtgcgcgctg acgagctggc cggtctggaa tcgccgcaga tcttcggcga
     5341 gcgcactcgc attcgcgtat cggtcggcgg gcacgcgagc catcgctttg ccgacgatgt
     5401 cgcacagctc ctgcgatgca cctggcgcag acgtcgcgag aggcgccggt ggtcctgcga
     5461 gcacgcggtc gagcacgccc tgcggagttg gatccgtata cgcgggcttt cccgcgagca
     5521 gctcgtagag caccgcaccg atcgcgtaca cgtccgcgcg ctcatcgacg agctcgccac
     5581 gcgcttgctc gggcgccatg tacgccggcg tgccgacgac cttgccagaa atcgtcgacg
     5641 cacccgagcc cttcgcgcgg gtctcgagca actcggtcgt cggctcgggc agatgcccgc
     5701 gtgtgtcacg cgcgagcccc cagtcgacga cgatggtctc gccgaactct ccgacgatca
     5761 cgttcgatgg tttgagatcg cggtgaatga cgccttcgct gtgtgcgtaa ccgacggcat
     5821 cggcgatcgc gatcacgtgc gcgagcagac cgaggcgatc gcggagcgtc ttcttttcgg
     5881 cgaacagctc cttgagcgtg cggccctcga cgagcttcat cacgtagtac ggggcgccgt
     5941 tgggccagcg acctgcttcg tgaacaggga cgatgcccgg atgctcgagg cgcgcggtga
     6001 tcaacgcctc gcgaacaaag cgcgcttcgt gcgactcgtc ttggcgcagc agctctttga
     6061 ccgcgaccgt tcgccccaaa cgtttgtcga cggcgcggac gacccggcca aggccaccgc
     6121 gcgcgtgctc gccgacatgc tcgtagcgat cggggctatc gatcgggagc tgggcctcgt
     6181 cgcccgttgc ttcttcgtgc cggggtgcct tcgaaggcat cgtcggctgc agggtcgcag
     6241 tgcggtcggc ggggtcggcg gacatggcga gggactagcg aaaacagcgc cggttcaacc
     6301 cagattccca tgactgagcc tcggttgaca tcaaagaaca gtgatacgac gggcggtgga
     6361 ggacagatac agatgactgg aaaggtgttg ctcgcagcaa ttggtgtcgc acttgtgtcg
     6421 cgccccgtcg cagcggatcc gaaaccggat tacgccgcta tcgcagagcg ggtcgtcggg
     6481 acgtcggcga atgtgaagga gggagagatt gtctggatca gcgtcggtcc gcaggacctc
     6541 gcgttcgcgg acgagctagc cgcagcggtg cgcaagcgcg gcgcgtttcc gatcgtcacg
     6601 tactcgagcg aggcgatgac gaagcgagtc ttcgcgacga tgcccgataa gtacgacgcg
     6661 caagcgccaa aaggtgacct cgagctgacg aagatcgcag ccacgcatat caacatcagc
     6721 tcggttcgcg atccgtcgat ctggctcgac ggcatttcgc cggagcgccg ccgaaaaatc
     6781 ttcgcggccg gcgcatcggc gcagagcctc gcggtgaagc gtcacgtgcg gttggtcgag
     6841 ctcgacaacg gtctcttgcc atccgcaacg cgcgcgaagg aaacgggact atcggaagcc
     6901 gagctcacga agcagttctg ggacggcgtc ggtgccgatt acacggcggt ctccgcgcag
     6961 tgcaagaagc tgacggatgc actcgcgaag ggcggcgagg tccacatcac gcatccgaac
     7021 ggcacggacg tgaagatgaa gctcaagccc aagaagacgg cggcgagtga cggcattctg
     7081 accgacgcta aggtgaaggc cggcggcccg ggcgtgcagg tctggttgcc ggcgggcgag
     7141 gtctacttcg tcccggtgat cagcagcgtc gagggcaagg tggtcgatga ccgcatggcg
     7201 atcttcggga aggagatcct cggccatagc ttcgacctca aggcgggcaa ggttgcgaac
     7261 cgcgcgacga agtcaggctg ggatgcgatc aaggcggact acgacgcggc cggccccggc
     7321 aaggacgagt tcggcatggt ggatctcggc tgtaattcag cggtgaagaa cacggccaag
     7381 ctcgagacgt ggatggctgc gggaacggtc agcatcacga cgggcaataa cgaatacgcc
     7441 ggcggcacga acaaggaacc gctcgggatg gcgcacatgc tcagcggcgc gacgctcacg
     7501 ctcgacggca aagcgctcat cgaaaatggc gctctgaaat agccgatcag cagcaggact
     7561 gaagcagctc gaacagcttc gagagatcga gcggctttcc catcaccgat gaacccgcag
     7621 gtgcgcgatg cggcgcggac gtcgtgatca acacgcgcag ggactcgagc tggccgcgcg
     7681 tctggagcgt ttcatagacg gcccagccgt cgacgttcgg catgaagagg tcgagcagta
     7741 cgatacagat cggcagctcg gcggagtcga tcgcgtccag tgcctgctgg ccatcggccg
     7801 ccgtgcgcac gttgtagcca cgaagctcga gcaactcgcg aagagtctcg cgcgcgtctt
     7861 cttcgtcttc gacaaccagc acgatcgggc ttactgccga cgccatgctg ccttttacca
     7921 ctagggcgga cgcgggagct cgatcgtgaa cgtggtgcct tcggtcttcg tagagcgagc
     7981 cgcaatggtc ccgccgtggg ccatgacgat ttgctgcgag atcgagaggc cgaggccgag
     8041 cccttctgac cggtggctgc gtgcatcggt gcggcgatac gcatcgaaca agaccggcat
     8101 gatgtcgggc tgaatcggct cgccggcgtt gtggatcgtg atccgcacgc ccgtgctcgt
     8161 gccttcgatc cgcgcctcga cgggagccgc cgagtcccca tgctggagcg cgttgccgag
     8221 aagattcgcg atcacctgcg ccatgcggct gccatcccac atgccggtcg tgttgccttc
     8281 taccttccgc cggatctcgc ggccaggata gagaagctcg agctcgccga tgatcgtgtc
     8341 gagcgtctcg gccagattga tctgctcgcg cgcgagcgtg atgccttcgc ccaagcgtac
     8401 gcgggtgagg tcgagcagct gcgacaccat cgcgctcatg cgcttggcac tgcggagaat
     8461 ccgctcggcg ttgaccttca ctgattcgcc gcccttctcg cggacgagcg cagcgccgac
     8521 cgtgatcgcg ttcaagggat tgcgcaagtc atggccgagc atgccgacaa actggtcggc
     8581 aaaccgcagc gactgctgga gcgacgtgaa ttgatcggcc gccatgatcg cgaggcccgc
     8641 gcggcctgcg aggtcttcga gcagcacctt gtcgtcctcg ccaagacgcg gctcgtcgac
     8701 gaagcgcgtc accgagagcg tcccgaggat ctctcctcgg gcgcggaccg ccaccgtgac
     8761 gaagcccttc accggatgct cctcgatgaa gcgaacgtgc tccggtcgca tggtgcccgc
     8821 gagagcggcg cgatcgatcg ccggtgtgag gacgctgatc ccggtctgcg cgacccggcc
     8881 cgcgatacct tcgccgagcg cgaacggctc gctgaggatg gcttgcatgc cggcctgcac
     8941 gtcgggatcg cggtggcgga gcgcgaccag ccgcacctgc ttcgcggcgc gatcgatcac
     9001 gtgcaacgcc gcgctgtcga agctgtgctc gacgacctgc tggacgacgg cctcgaagat
     9061 cgccgcgcgg tcgagtcctg cttgcgtgaa cgcgcgcgac gcctgcacga gcacgctcat
     9121 gcgctgcccg aactgctctg cgacgcgacg cgcttggacc tgctcggtga tgtccatgcc
     9181 gacgacgatg acgccccgag tctcgtcgtc gatgttcatc ggctgatacg tcgcggtgaa
     9241 gtagcggtcg cgtaatacgc cgtctgcatc gcgtaggggc gcgacgtact cgggcagcgt
     9301 ccgcacttcg cccgtcgcaa gcgcgtcctg aatcacgtga aacgtcggac gatgccgaag
     9361 ctcggggacc gcctcgagaa gaggcttgcc acgcacgtcg cggtcaccgg tcactgcgat
     9421 atacgcgtcg ttggcgagca ccacgcgcag ctcttccttc gcatacagcg ccatcggaat
     9481 cggcgcctgc atgatcacgg cgcgcagcgt ctcgcgctcg cgttcggctt cttgctgagc
     9541 cgcgccgagg tcgctgttca gctgctcgag gcgcagtcgc tggcggcgct gcaacagatc
     9601 gacgacggtt ccgagcgaga cgacacatgc agtaaatcgc agcacgtgaa acgcccacca
     9661 cgtgccgtcc catggctgcg agaacccgaa catcagggcc gctagcgcga ggagcaccgc
     9721 atagctcgca aacacggcgt cgatgaagct gccgcgccgc cgttgctcgc gaagcatgaa
     9781 caccgctgcg gcgacgaagc ctgcgcccgc gacctggttg atgtgctgtg cccaaggccg
     9841 atagttgaat ccgcgctcga acgcagatgg ccaggcattc ggggccgcca cgagcaggat
     9901 cccgagcgtg acggcaatcc ccaagacgac cgcagggagg atcgggacgc gccgccgcgg
     9961 caaccatacg agcgcgaaca gcagtgcgcc aagcacggtc ggcaccgtgc gcgtccaata
    10021 gaacgtggcg ccgggcggag taatggcgtg gacgatgtcg aggacgcctt gggcaatcag
    10081 gctcgcggcc acccactcga ggtaggggcg gtcttcgagg gcgctgccac gcagcagcaa
    10141 tgtcgccatt gcaagggcca ctacgccgcc tacagcctcg atcgtcgagt gcaggggctc
    10201 gttcacgctg cgcccgtcga cggcgcccga caccaccccg accgtgatcg cggccacggc
    10261 gacggtcagc agggggttta ggagcgtcca gcgccagctc tgtcgcacaa accggtacgt
    10321 acgatacccc gccgttgccc gagtctcacc gagcggtcac aggttccgcg cgaaaattgg
    10381 gctctgtagt tggcggcgcc tgcgttagag tccgcgccca tgcagagggc ggtccttgcg
    10441 accatcatcg tgacgagtgc gtgtggaccg tcggggcgag acggtaatgg cgatttgccg
    10501 gacgcgcgtc cgtcgggcga tggtcaggtc acgctgccag cgtcgcgcgt ctacgcgcac
    10561 agcggaagca agctgtacca agtcgactcg cagccgccgc acacgcctgt cgagatcgga
    10621 acgatgggcg gcatcggcac gcagagcctc accgacctcg cgatcgacaa gacggatcgc
    10681 atggtcggca tcacgctcga caagctctac acgatcaacc cgacgacagg cgccgcgacg
    10741 ctcgtcaccg atctgtcgac caccgcgcgc ggcttcacga gcctctcgtt cgtgcccgcc
    10801 gacatcaaca atccgtcgag cgctgacatc ctcgtgtcag ccaacgacca gggcgacgtc
    10861 tactcgatcg acgcgatgaa cggcaacgcg acgaagatcg gcagctacgg ctcggtcagc
    10921 gcgggcaagg tcatctcgag cggcgatctc atcggcgtgc agggcctggg catctacgcg
    10981 accgtcgacg tcggcacgca gccgaacgac tacctcgcgc gcatcgatcc gacgacgtgg
    11041 aaggccacgc cgatcggcac cgcgagcacc ggctacgaca acatcttcgg cctcgcgttc
    11101 tgggagggga agatctacgg cttcgtctcg gcgggcaccg acgccggcaa gatcatcacg
    11161 atcgatccga cgaccggcgt gggcacgcag gtgcagagtg gcgccgtgcg ctggtacggc
    11221 gcgggcgtcg cgaccgacgc tccgatcatt cagtagcagg ctcgaccatg ggcggtggga
    11281 cgggcttgcc gtctcggcgc gagaagtcga cggcctgcgt gcggtcgggc acgacgtctt
    11341 tgtgcgcctc gcgcgggacc atttgctcgc cgcccttctt gtagatgcgc tgctccgtcc
    11401 acgtcgcgtc gagtgaaatt ggcttcgtgc tcttcggcac gacgaacgac agcggcgtct
    11461 tgccgatggt cttgccgtcc ttcgtgatcg tcatctgagg aagcgcgcgg acttcgatgc
    11521 ggactgtcgt ttccgacgac gattcggagc tctgaccgcg catcacaaac actgcaacgg
    11581 cgacggaccc gaggaccacg gcgacgatcg cgccgacgat gattttgagc tggcgggaca
    11641 tctcgcgcgc ggagcctacc gcgctatggt ccgcgcatga cactcccgat ccgccgcgtc
    11701 gccgttctcg gtgcgggcgt catgggcgcg ggtatcgccg cgcactgcgc caatgcaggc
    11761 ctccccgtcc tgctcctcga catcgtcaaa cccgacctcg acaagctgaa gaaggccaag
    11821 ccggccgcgt tcatgcacaa ccgcaacgcg cagctgatcg agacgggcct cttcgatacg
    11881 cacctcgaga aggtcaaaga ctgcgacatc atcatcgaag cgatcatcga gcgcctcgac
    11941 atcaagcagc agatgttcga gaagctcgag aagatcgcgc cgaacgcgat cgtcgcatcg
    12001 aacacgtcgg gtctgcgcat cgccgacatg ctgcaaggac gcagcgaagc cttccgcaag
    12061 aacttcgtcg tcacgcactt cttcaacccg ccgcgctaca tgaagctgct cgagctcgtc
    12121 gccggtccgg acaccagcgc cgaagccaag cagcgcgtcg aagcattcgg ccgcgacgtg
    12181 ctcggcaaag gcatcgtgtg gggcaaggac acgccgaact tcgtcggtaa ccgcatcggc
    12241 gtgcagtcga tgctgacgac gatccacttg atgatcgaga agggcctgt
//
LOCUS       DATLBZ010000322         8886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9305393, whole genome shotgun sequence.
ACCESSION   DATLBZ010000322 DATLBZ010000000
VERSION     DATLBZ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9305393"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VMZ53_16925"
     CDS             <1..252
                     /locus_tag="VMZ53_16925"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HUS30195.1"
                     /translation="RDGLIASDVVVVDLDDELIAQYVASGEWKGKAGAYAIQGIAAAF
                     VREVRGSVTNVIGLPLVEVLEALRAVGAPAGNLSAGVPA"
     gene            249..926
                     /locus_tag="VMZ53_16930"
     CDS             249..926
                     /locus_tag="VMZ53_16930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HUS30196.1"
                     /translation="MSIAERWAEVRARVDAACEKAGRAPSEVTIVAVSKTHPAAAVRE
                     AAAAGALDFGENYAQELAVKRGELTDVAVRWHYIGRLQRNKSKLVAGHVALVHAVDSV
                     ELATELGKRSPLVQPILLAVNAAGEETKGGVTHETAGELARAIAGVANVRLDGLMTMP
                     PPADDPEASRPFFVALRELRDRIADELGRPLPVLSMGMSGDYEVAIACGSTHVRVGTA
                     IFGAREG"
     gene            936..2168
                     /locus_tag="VMZ53_16935"
     CDS             936..2168
                     /locus_tag="VMZ53_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30197.1"
                     /translation="MKGVLIAIVLVLAGCTAILDLHGVDSVDHDNDNVPDAIDNCPDA
                     ANPDQSDFDHNGKGDACDLCTDGGAEDVDQDGIPDGCDGCIGRGEDVDQDGIDDGCDA
                     CLGSGEDADHDNIDDACDACVGNGMDLDHDNIDDKCDTCVQKFTDTDGDSLDDGCDPC
                     VASPPGVDFDHDGIEDACDPCAFGPQHDEDKDGVYDACDNCDVIANPGQANGDNDGCG
                     DDCDIGTDSDRENADAFAAQNASWYLLGSGWTLGNDVAQLRTTGDSARWLSQVTPDFE
                     LKTRVVYTQFGPNILPGKVSLIASEVGTLSPQHLLACVIDTNGTLTAIATFPTTGTQT
                     NTAPMTFNSSGTVTLRMRASSKKDTMTCFAEVSGIIAFDVMASVTITEKSVPWSAGLA
                     GTHVDATFDYFDQIFYAP"
     gene            complement(2169..3146)
                     /locus_tag="VMZ53_16940"
     CDS             complement(2169..3146)
                     /locus_tag="VMZ53_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30198.1"
                     /translation="MRAICIVVILLGVANAQPLPRAEDKAEADRLFEEGRALLEKGDR
                     AEACKRFDLSFRKDPRAVGTMLNLGLCREEAGLVASAVRYYQEARDRARDQNLAEHQQ
                     AAERKIAMLSPRVPRLTIALAPGTEATARVLVDDTVIAQDQLIDVTVDPGDHALVVTA
                     PGKLPYEAKLTVAESEHRKVDVPALQGATIVVREKSNRRFVGKVGVGVGAGLLVASVG
                     LGLYARHGYWSQFPDGARDGEIVRDDDHDCWTVLDNGKVARQCNEVGANALDNARLVA
                     HISTGAAIAGGVAVLAGAFLWATAPRQEAPPLALDVGPDHATVTVHAQF"
     gene            3201..3722
                     /gene="ruvC"
                     /locus_tag="VMZ53_16945"
     CDS             3201..3722
                     /gene="ruvC"
                     /locus_tag="VMZ53_16945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HUS30199.1"
                     /translation="MRILGLDPGSRVCGYGVIDARGDALAYVECGVLTAPEDRPMEFR
                     LGEIARGLREVIAELAPNVVAVEDVFVHQRNPRSGLALAQARGMALAVIGLSGLTVAS
                     YPPALVKKSVAGSGGAGKDQVARMVRALIGLNSLPKADATDALAIAITHGRTVRPTAV
                     KIVSAVRTARSNV"
     gene            3719..4372
                     /gene="ruvA"
                     /locus_tag="VMZ53_16950"
     CDS             3719..4372
                     /gene="ruvA"
                     /locus_tag="VMZ53_16950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HUS30200.1"
                     /translation="MIARLEGIVIEKDPPHVVIGCNGVGYDVICSGYTMTELPADGER
                     VTLRVFTHATENKLSLFGFIDRQERALFDLLITVKNVGPSTAIAILSGSNPRDIATLI
                     AAEDVPGLTRIKGVGKKTAELLVVELKDKAAEALLMWNDTGGLRPAALPSSIRIQGKT
                     KRHPVIEEVATALVGMGWRPQEADQAVVELPIPDDAKPNDPALIAQLLRQALRSMPR"
     gene            4380..5459
                     /gene="ruvB"
                     /locus_tag="VMZ53_16955"
     CDS             4380..5459
                     /gene="ruvB"
                     /locus_tag="VMZ53_16955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HUS30201.1"
                     /translation="MTRKKHIDLVRDEDVAREVAPVEKGGEGAWQQALRPRNFEEYIG
                     QRELVDNLRKSVRAAKEQGWVLDHFLFAGPPGLGKTTLAHVIANELGVNLHVSSGPAI
                     DHKGKLAALLTNLGERDALFIDEIHRLHPVVEENLYPAMEDFRFDLFIGDGPHAKAVT
                     MQLPRFTLLGATTRMGLLSAPLLDRFGFHWQLRYYDLADMKSIVKRSASLIGVPIDEA
                     GATEIARRSRGTPRIANRLLRRVRDFAVVDHDGSIDGTLAGDALDQLKVDHIGLDDLD
                     RRYLQVVCERFDGGPVGVEAIAASLSEERGTLEEVVEPFLLQVGLIARTPRGRVATAS
                     GFQHMGLAAPSKAPTGGSTAQGRLF"
     gene            5469..6314
                     /locus_tag="VMZ53_16960"
     CDS             5469..6314
                     /locus_tag="VMZ53_16960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS30202.1"
                     /translation="MNGKTVVITGASSGIGKITAHELARKGARIVMVVRNRDKGQGVI
                     DEIVRAVPDAQLELAIADLYSLGDVRRAGAEIRGKHPKIDVLVNNAGLIHDKRELTVD
                     GFERTFALNHLAPFVLTYELLGSLAAAAPARIVTVSSEGHKFSRIDWNQLATASDWKL
                     ATLTYGNSKLCNIWFARESARRLVTARITSNALHPGPVATGFGQSGSIIYRLGTKLAK
                     PFLLTPEEGARTQIYLASSPDVEGVTGEYFIKCRIAKPSSAARDDASARRLWELSEKL
                     TGVSY"
     gene            6424..7083
                     /locus_tag="VMZ53_16965"
     CDS             6424..7083
                     /locus_tag="VMZ53_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30203.1"
                     /translation="MRIFSVLVCLLAACGARIGGDDSDDNSARPDASISRSPDAGMQQ
                     QQPDAAPVVMDNACGVAQNQGDLGNLTGFAGTQLQDGSTTQRIRWVGSETAATANQAS
                     PDVLYVELWDGFGAFTGGAARTGTFTISGNETDLDTCGVCVMMLANVTNNTPAKTLVA
                     TSGTVTITSVGTTAGQMTQATVTNASFTEVTCNQTNGCTNVAGSTCTSPISHGDVRGT
                     L"
     gene            complement(7159..7695)
                     /locus_tag="VMZ53_16970"
     CDS             complement(7159..7695)
                     /locus_tag="VMZ53_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30204.1"
                     /translation="MGKWFVVACLFVAACRPMYGDPAPHLKDPPHRNPPKGWNDEPVA
                     EKVYVEECNVDFSAPPTTKRQTKAAEQKVVAGDSAAAESERATDPKARVDTTKRSLQE
                     YRDALIADPFNAQATLKLALAYDKVLRKGCAIAMLKRLDKLAENPKFNAEPVKDQVVD
                     NEQWFKPYRNEALRAIGH"
     gene            7720..>8886
                     /locus_tag="VMZ53_16975"
     CDS             7720..>8886
                     /locus_tag="VMZ53_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30205.1"
                     /translation="MRLPVLLLAVVACSSTKQPPPPAPMKERVATNTVGESNIERADY
                     IGPDACAECHPQQHAMWSASLHRVMNARASDAGAVIADFATEVHYRDGVASFGRSLGG
                     LVMTLNDGGREVRYNVTRTIGHRGLQEFVGVEDGRTEEVRLPFGWWPRAGGWVPQVAF
                     DPWLDEAAFDPFAPVREPWAERCPWCHSTYPFAQRIARSSVKQVGHGLEQYVERAASS
                     GNPERLAVDEQVTIGISCESCHLGGRAHADGAKISSLPAGAGVHVRADAPAMPATFEL
                     QRKDAHVVNTICAQCHSGPSPRLADGTALRNSSEALDLAAGPCTTLKCTDCHDPHVAD
                     AVPDETRAIATCKGCHVAQAAPAHAGQGHAGTTCLDCHMPRVVMGVDSYVRTHRI"
BASE COUNT         1504 a         2932 c         3009 g         1441 t
ORIGIN      
        1 cgcgatggcc tcatcgccag tgacgtcgtc gtcgtcgatc tggacgacga gctgatcgcg
       61 cagtacgtcg cgagcggcga gtggaagggc aaggcggggg cctatgcgat ccagggcatc
      121 gccgccgcgt tcgtgcgcga ggtgcgcggc tcggtgacga acgtgatcgg cctaccgctc
      181 gtcgaggtgc tcgaggcgct gcgtgcggtc ggcgcgccgg cgggcaatct ctcggcgggc
      241 gtgccggcat gagcatcgcg gagcgctggg ccgaggttcg tgcgcgcgtc gatgcggcgt
      301 gcgagaaggc agggcgcgcg ccgagcgagg taacgatcgt tgcggtcagc aagacgcatc
      361 ccgccgcagc ggtgcgtgaa gcagccgccg cgggcgcgct cgacttcggc gagaactacg
      421 cgcaggagct ggcggtcaag cgcggagagc tgaccgacgt cgcggtccgc tggcactaca
      481 tcggccgctt gcagcgcaac aagtcgaagc tcgtcgcggg gcatgtcgcg ctcgtgcatg
      541 ccgtcgactc ggtggagctc gcgacggagc tcggcaagcg atcgcccctg gtgcagccga
      601 tcttgctcgc ggtcaatgcg gcaggcgaag agaccaaagg cggcgtgacc cacgagaccg
      661 ccggcgagct cgcgcgcgcg atcgcaggcg tcgcgaacgt gcggctcgac gggctcatga
      721 cgatgccgcc gccggcggat gatccagaag ccagccggcc gttcttcgtc gcgctgcgcg
      781 agcttcgtga tcggatcgcg gatgagctcg gccggccgct gccggtgctc tcgatgggca
      841 tgagcggcga ctatgaggtc gccatcgcgt gcggctcgac acacgttcgc gtcggaaccg
      901 cgatcttcgg cgcgcgcgaa gggtaagata gcgaagtgaa gggcgtgctc atcgcgatcg
      961 tgctggtgct cgcaggatgc accgcgatcc tcgatctgca cggcgtcgat tcggtcgatc
     1021 acgacaacga caacgttccc gacgcgatcg acaactgccc cgacgccgcc aatcccgatc
     1081 agtcggactt cgaccacaac ggcaaaggcg atgcgtgcga tctgtgcacc gacggcggcg
     1141 ccgaggacgt cgatcaggat ggcattcccg atggctgcga cggctgcatt ggacgcggcg
     1201 aggacgtcga ccaggacggc atcgacgacg gctgcgacgc gtgtctcggc agcggcgagg
     1261 acgccgatca cgacaacatc gacgatgcgt gcgacgcgtg cgtcggcaac gggatggatc
     1321 tcgatcacga caacatcgac gacaagtgcg acacgtgcgt gcagaagttc accgacaccg
     1381 acggtgactc gctcgacgac ggctgcgatc catgcgtcgc ttcaccgccc ggcgtggact
     1441 tcgatcacga cggcatcgaa gatgcgtgtg acccgtgcgc gtttggcccg caacacgacg
     1501 aggacaagga cggcgtctac gatgcgtgcg acaactgcga cgtcatcgcg aaccccgggc
     1561 aagcaaacgg tgacaacgac ggctgcggcg acgactgcga tatcggcacc gacagcgatc
     1621 gcgagaacgc cgatgccttc gcggcgcaga atgctagctg gtacctcctc ggcagcggct
     1681 ggacactcgg caacgatgtc gctcagctgc gaacgaccgg agattccgct cgctggctct
     1741 ctcaggtaac acccgatttc gagctgaaga cccgggtcgt gtatacgcag ttcgggccga
     1801 acatcctccc cggcaaggtg tccttgatcg catccgaggt cggcacgctc tcgccgcaac
     1861 acctgctcgc ctgcgtgatc gacacgaacg gtaccttgac cgcgatcgcg acgtttccga
     1921 cgacgggaac gcaaacaaac accgcgccga tgacgttcaa ctcctcgggg acggtcacgc
     1981 tgcggatgcg cgcgagctcc aagaaagaca cgatgacgtg cttcgccgag gtctccggca
     2041 tcatcgcctt cgatgtgatg gcgagcgtca cgatcaccga gaagagcgtg ccgtggtctg
     2101 cgggcctcgc gggcacccac gtcgatgcga ccttcgacta cttcgatcag atcttttacg
     2161 cgccgtaact agaactgcgc gtggacggtg accgtcgcgt ggtcgggccc gacatcgagg
     2221 gcgagcggag gagcctcttg gcgtggagcg gtggcccaca agaacgcgcc tgcgagcacg
     2281 gcgactccac cggcgatggc cgcgcccgtc gagatatgcg cgacgagccg cgcgttatcg
     2341 agcgcattcg cgccgacctc gttgcattgc ctcgcgactt tgccgttgtc gagaacggtc
     2401 cagcaatcat ggtcgtcgtc gcggacgatc tcgccatcgc gcgcgccgtc ggggaactga
     2461 ctccagtagc cgtggcgtgc gtagaggccg aggccgaccg atgcgacgag gaggcccgca
     2521 ccgacgccga cgccgacctt gccgacgaag cgccgattgc tcttctcgcg cacgacgatc
     2581 gtcgcgcctt gcagcgcggg gacgtcgacc ttgcgatgct cgctctcggc gaccgttagc
     2641 tttgcctcgt atggcagctt gcccggtgca gtcacgacga gcgcgtgatc gcccggatcg
     2701 accgtgacgt cgatcagctg atcttgtgcg atgaccgtgt cgtccacgag cacgcgcgcg
     2761 gttgcctccg tgccgggtgc gagcgcaatc gtcaagcgcg ggacgcgcgg ggagagcatc
     2821 gcgatcttgc gctccgccgc ctgttggtgc tcggcgagat tctgatcccg cgcgcggtcg
     2881 cgagcttcct ggtagtagcg gaccgcgctc gcgacgagcc cggcttcttc gcggcagagc
     2941 ccgaggttca gcatcgtgcc gaccgcgcgc gggtctttgc ggaacgagag gtcgaaacgc
     3001 ttgcaggcct cggcgcgatc gcccttctcg agcagcgcgc gaccctcttc gaacagccgg
     3061 tcggcctccg ccttgtcctc ggcgcggggc aacggctggg cgttcgccac tcccagcaga
     3121 atcacgacga tgcagatcgc cctcaccccg aaaaggctaa catttggccc gctgtcggag
     3181 ggtcgaagta gtgtctatcc gtgcgaattc tcgggctcga ccccggctct cgcgtgtgcg
     3241 gatacggcgt catcgacgcg cgtggggatg ccctcgcgta tgtcgagtgc ggcgtgctga
     3301 ccgcgcccga agaccgtccc atggagtttc ggctggggga gatcgcgcgg ggcctgcgcg
     3361 aagtgatcgc ggagctagcg ccgaacgtcg tcgccgtcga ggatgtgttc gtgcatcagc
     3421 gcaatccacg cagcggtctc gcgctcgcac aagcgcgcgg catggcgctc gccgtgatcg
     3481 ggctctcggg gctcacggtc gcttcgtatc cgccggcgct cgtgaagaag tccgtcgcgg
     3541 gcagcggcgg cgcaggcaaa gaccaagtcg ctcgcatggt gcgcgcgctg atcgggctca
     3601 acagcttgcc gaaggccgac gccacggatg cgctcgcgat cgcgatcacg catggccgca
     3661 cggtgcgccc gaccgcagtg aagatcgtct cggccgttcg tacggcaagg agcaatgtgt
     3721 gatcgcgcgc ctcgaaggaa tcgtcatcga gaaagatccg ccgcacgtcg tgatcggctg
     3781 caacggtgtc ggctacgacg tcatctgctc tggctacacg atgacggagc tgccggccga
     3841 tggtgagcgc gtcacgctgc gcgtgttcac gcacgcgacg gagaacaagc tgtcgctgtt
     3901 cggcttcatc gatcggcaag agcgtgcgct gttcgacctt ctgatcacgg tgaagaacgt
     3961 cggaccgtcg acggcgatcg cgatcttgtc gggcagtaat ccgcgcgaca tcgcaacgct
     4021 gatcgccgct gaagacgtgc cgggcctcac gcgcatcaag ggcgtcggca agaagaccgc
     4081 ggagctgctc gtcgtcgagc tcaaggacaa agcggccgaa gcgctgctga tgtggaacga
     4141 caccggcggc ctgcgtcccg ccgcgctgcc gtcgtcgatc cgcatccagg gcaagacgaa
     4201 gcgccatccg gtgatcgaag aggtcgccac cgcgctcgtc ggcatgggct ggcgcccgca
     4261 agaggccgac caagccgtgg tcgagctgcc gattcctgac gacgcgaagc cgaatgaccc
     4321 cgcgctgatc gcgcagctgt tgcggcaggc gctgaggagc atgccaaggt gacccgccga
     4381 tgacgaggaa gaagcacatc gatctagttc gcgatgaaga cgtcgctcgc gaggtcgcgc
     4441 cggtcgagaa gggcggcgag ggtgcgtggc aacaagcgct gcggccgcgc aacttcgagg
     4501 agtacatcgg ccagcgcgag ctcgtcgata acctccgcaa gagcgtgcgc gcggcgaaag
     4561 agcagggctg ggtcctggat cactttctgt ttgcggggcc gccaggcctc ggcaagacca
     4621 cgctcgcgca cgtcatcgcc aacgagctcg gcgtgaacct gcacgtcagc tcggggcccg
     4681 cgatcgatca caagggcaag ctcgctgcac tgctcacgaa cctcggcgaa cgcgacgcgc
     4741 tgttcatcga cgagattcat cgcctgcatc ctgtcgtcga ggagaacctc tatccggcga
     4801 tggaggactt ccgcttcgac ctcttcatcg gcgacggccc gcacgcgaag gccgtcacga
     4861 tgcagcttcc acggttcacg ctgctcggcg cgaccacgcg catgggcctg ctctccgcgc
     4921 cgctgctcga tcggttcggc ttccactggc agctgcgcta ttacgacctc gcagacatga
     4981 agtcgatcgt gaagcggtcg gcgtcgctaa tcggtgtgcc gatcgacgag gcaggcgcga
     5041 cggagatcgc tcggcgttcg cgcggcacgc ctcgcatcgc gaatcgcttg ctccgccgcg
     5101 tgcgcgactt cgcggtcgtc gatcacgacg gctcgatcga cggcacgctc gcgggcgacg
     5161 cactcgatca gctcaaggtc gatcacatcg gcctcgatga tctggatcgc cgttatctcc
     5221 aagtcgtgtg cgagcgcttc gatggcggcc cggtcggcgt cgaggcgatc gccgcatcgc
     5281 tgtcggaaga gcgcggcacg ctcgaggaag tcgtcgagcc gttcctgctc caagtcggcc
     5341 tcatcgcgcg cacgccccgc ggccgcgtcg ccaccgcgtc gggattccag cacatgggcc
     5401 tcgccgcacc gagcaaggcg ccgacgggcg gctcgacggc acagggccgc ttgttctagc
     5461 ctgcgggcat gaacgggaag acggttgtca tcaccggagc aagctccggg atcgggaaga
     5521 tcacggcgca cgagctcgcg cgaaaaggcg cgcgcatcgt gatggtcgtg cgcaaccgcg
     5581 acaaggggca gggcgtgatc gacgagatcg tccgtgctgt tcccgacgca cagctcgagc
     5641 tcgcgatcgc agatctgtac tcgctcggcg acgtccgccg cgccggcgcg gagattcgcg
     5701 gaaagcatcc caagatcgac gtgctcgtca acaacgcggg cctcatccac gacaagcgcg
     5761 agctgactgt cgatggattc gagcgcacgt tcgcgctcaa tcacctcgcg ccgttcgtgt
     5821 tgacgtacga gctgctcggt tcgctcgcgg ctgcggcgcc ggcacgcatc gtcacggtct
     5881 cgtccgaggg ccacaagttc tcgcgcatcg actggaacca gcttgcaacc gccagcgact
     5941 ggaagctcgc aaccctgacg tatggcaact cgaagctctg caatatctgg tttgctcgcg
     6001 aatccgcgcg gcgcctggtc accgcccgca tcacgtcgaa cgcgctgcat cccggaccgg
     6061 tcgcgactgg ctttgggcag agcggctcga tcatctaccg cctcgggacg aagctcgcga
     6121 agccgtttct cctcacgccg gaggaggggg cacgcacgca gatttacttg gcgtcctcgc
     6181 ccgacgtcga aggcgtcacc ggcgaatatt tcatcaagtg ccgcatcgcg aagccgtcgt
     6241 cggcagcccg cgacgatgcc agcgcccgcc gcctctggga gctctccgaa aagctcaccg
     6301 gtgtctccta ctaggagacc ctagcgggtg gggctctccc cactttgtga cccgccgtcg
     6361 gcacgaatcc tcggcgagcg cgcgtggtta cgtctcggta cggcgcttgc tcaacacgcg
     6421 cacgtgcgca tcttttcggt cctcgtctgc ctactcgccg cctgcggagc ccgcatcggc
     6481 ggagacgaca gcgacgacaa ttcggcacga ccggacgcga gcatctcgcg gagcccggac
     6541 gccgggatgc agcagcagca acccgacgcg gcgcccgtcg tcatggacaa cgcatgcggc
     6601 gtggcccaga accaaggcga cctcggcaac ctgaccggct ttgcgggcac gcagctgcaa
     6661 gacggctcga cgacgcagcg catccgctgg gtcggctccg agaccgcggc gaccgcgaac
     6721 caagcctcgc ccgacgtcct gtacgtcgaa ttgtgggacg gctttggcgc attcaccggc
     6781 ggcgccgcgc gcaccggtac gttcacgatc agcggcaacg agaccgacct cgacacgtgc
     6841 ggcgtctgcg tgatgatgct cgcgaacgtt acgaacaaca cgcccgccaa gacgctggtc
     6901 gcgacgtcgg gcacggtgac gatcacgtcg gtcggcacca cggctggaca gatgacacag
     6961 gcgaccgtca ccaacgcgag cttcaccgag gtcacgtgca accagaccaa cggctgcacg
     7021 aacgtcgccg gctcgacctg cacgagcccg atctcgcacg gcgacgtgcg cggcacgctc
     7081 tagcgaacaa tgcgcggaca ctcgcgcgcc gcactcggct gctccaaaat gaagcagtgg
     7141 ggtcggaccc ctagtccgtt agtggccgat cgcgcgtagc gcttcgttgc gatacggctt
     7201 gaaccactgc tcgttgtcga cgacctgatc cttgacgggc tcggcgttga acttcgggtt
     7261 ttcggcgagc ttgtcgaggc gcttgagcat cgctatcgcg cagcccttgc gaagcacctt
     7321 gtcgtacgcg agcgcgagct tcagcgtcgc ttgcgcgttg aacggatcgg cgatcagcgc
     7381 gtcgcgatat tcttggaggc tgcgcttcgt ggtgtcgacg cgggctttcg gatcggtcgc
     7441 gcgctcgctc tcggccgcgg cgctgtcgcc agcaacgacc ttctgctcgg cggccttcgt
     7501 ctgacgcttc gtcgtcggtg gtgcgctgaa gtcgacgttg cactcttcga cgtagacctt
     7561 ctcggcgacg ggctcgtcat tccaaccttt cggcggattg cgatgcggcg gatccttcag
     7621 gtgcggcgct ggatctccgt acatcgggcg gcaggcggcg acgaacaggc aagcgacgac
     7681 aaaccatttc cccatggccg caagtatagt cgggcgccct tgcgattgcc ggtccttttg
     7741 ctcgcggtcg tcgcctgctc gtcgacgaag cagccgcctc cgcctgctcc catgaaggag
     7801 cgcgtcgcca cgaacaccgt cggggagagc aacatcgagc gtgcggacta catcggcccc
     7861 gatgcgtgcg cggagtgcca cccgcaacag catgcgatgt ggtcggcgag cctgcatcgc
     7921 gtgatgaacg cgcgcgcgtc cgatgcgggc gcggtgatcg ccgactttgc aacggaggtt
     7981 cactatcgcg acggcgtcgc gagctttggt cgctcgctcg gcggtctcgt gatgacattg
     8041 aatgatggcg ggcgcgaggt ccgctacaac gtcacgcgca cgatcggaca tcgtgggctg
     8101 caggagtttg tcggcgtcga ggacggacgc acggaggaag tgcgcctgcc gttcggctgg
     8161 tggccgcgcg ccggtggctg ggtgccgcag gtcgcgttcg atccatggct cgacgaggcc
     8221 gcgttcgatc cattcgcgcc ggtccgcgag ccatgggcgg agcgctgccc gtggtgtcac
     8281 tcgacgtatc cgttcgcgca gcggatcgcg cgctcgtcgg tgaagcaagt cggtcatggc
     8341 ctcgagcaat acgtcgagcg agcggcgagc agcggcaatc ccgagcgact tgcggtcgac
     8401 gagcaagtga cgatcggcat cagctgcgag agctgtcacc tcggcgggcg cgcgcatgcc
     8461 gacggcgcga agatctcgtc gctgcccgcg ggcgctggcg tgcacgttcg cgcggacgca
     8521 cccgcgatgc ctgcgacgtt cgagctgcag cggaaggatg cgcacgtcgt caacacgatc
     8581 tgcgcgcagt gccactcggg tccatcgccg cgcctcgcgg acgggacggc gctgcgcaac
     8641 tcgagcgagg cgctcgatct cgccgcgggc ccgtgcacga cgctgaagtg cacggactgc
     8701 cacgatccgc atgtcgccga tgcggtgccc gacgagacac gcgcgatcgc gacgtgcaag
     8761 ggctgtcacg tggcgcaggc ggcgccggcg catgccgggc aggggcacgc ggggaccacg
     8821 tgcctcgact gccacatgcc gcgcgtggtg atgggcgtcg acagctacgt gcggacacac
     8881 cggatc
//
LOCUS       DATLBZ010000323         7438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4078423, whole genome shotgun sequence.
ACCESSION   DATLBZ010000323 DATLBZ010000000
VERSION     DATLBZ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4078423"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VMZ53_16980"
     CDS             complement(<1..775)
                     /locus_tag="VMZ53_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30206.1"
                     /translation="MGEGPAGSRKMITRVGWHGSARDEESRAYLQARLTVLSKLAFWS
                     FIALLGGMKVLYWRYPLIEPVDNNLIYLIATVGLVIEAIIWRGFLVRRQLRLEWLYAI
                     DLFYALATGIIFGASTFIASDLQSSAFANMLWSCFMIFLRAIVVPSTGRRTAIAGVLL
                     FLPLNLAGVGQALWRNKHIELPADAYVGSALVISCAVIVLVTIGSNVIYGLRKQASAA
                     MRLGQYTLDRKIGEGGNGAVYRAHHAMLRRPTAIKLVHPD"
     gene            complement(791..1306)
                     /locus_tag="VMZ53_16985"
     CDS             complement(791..1306)
                     /locus_tag="VMZ53_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30207.1"
                     /translation="MTPLHKLYAKLCRELAQSEHSAMIHTRREARRLGDVPPAAALRS
                     LGAHAASLRPRMSALMTTRQPIGVVAARTVGQIFSTLRHLVFDRMIDVERSYRGTLLG
                     FHHGIATARLLREVSQRIGDRHMAGFCDDLLAQRVPLLNAAEQEVTWFAGTPKKAIKS
                     GLRLAFEPAAE"
     gene            1407..1811
                     /locus_tag="VMZ53_16990"
     CDS             1407..1811
                     /locus_tag="VMZ53_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30208.1"
                     /translation="MKRVKRMLWSAGLTMGFGALVAAVIQRYRQLPANDTFADDESGV
                     DSGKRETVMQHNIAVNVGDQVFLEEGGEEIGAVRKVERDHLVVYIEAAGDFVIRGPEI
                     TAVHHGKVILAPDKVEGRLLDAARGAHEREVF"
     gene            1921..2526
                     /locus_tag="VMZ53_16995"
     CDS             1921..2526
                     /locus_tag="VMZ53_16995"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HUS30209.1"
                     /translation="MTQHSATMRLLDEETRDLHADVDAYWLDLMASGVTKDSYRAQLI
                     RVFGFEAPFESALAYTPHLIVPDRRDLTRSGLIAQDLLALGITPAKITALPQCPEIAP
                     FEDPAEALGWKYVAERPTQLHSAIKRNIVSRVADSANALAYLSAYDGVAAARWQQLGV
                     LLENVAARPGAQSKLVKAAKTAFTCMGAWFRAHKNHDHAAL"
     gene            complement(2587..3567)
                     /locus_tag="VMZ53_17000"
     CDS             complement(2587..3567)
                     /locus_tag="VMZ53_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30210.1"
                     /translation="MKRALLLLVLAACPAKKGTGTGPTAQGAGCPSAKNVLVASFVQQ
                     DPGKGRSGWVIPLHAQAAQDASQVPEYAQLDAQAASAAGVPAAPQGALWLVTQTAPPC
                     PVKLGRYYQARIDGPPASATFGVEIEGCPAPANPDEAGGFVLVSDAAPTGCRFEPPQP
                     VAARLGEMDKAKQWQRPAKETPLPPAIAAIVPPHTCTPPACETLWAFAEVKVNGATVA
                     WTGAVNWLQVGSPADQCSWPNERWSGVFVPSGSGAIKISEGQSHPLTLLATLVDNTGA
                     RVLLTEGPGEFATYDVAQGSAPLGNHVSWMTVPETAWETVDDLGPICDAP"
     gene            complement(3583..4209)
                     /locus_tag="VMZ53_17005"
     CDS             complement(3583..4209)
                     /locus_tag="VMZ53_17005"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS30211.1"
                     /translation="MQRQVRTTPRKRPRQERSKATVDTILEATTRVLVKQGFDGLSTN
                     AVAAQAGVSIGSLYQYFPNKEALVSALIDRHMEDMNAAILAELTRVAKLPLAEAARAV
                     VELTIRAHSIDPELHRVLTEQVPRIGKLARLRELDEICHRMVAGLLAARKDELAIRDP
                     DLAAFILVSAIEAVVHRAALLYPARLRDPRLVDETTLLVTRYLGLAAN"
     gene            4331..5626
                     /locus_tag="VMZ53_17010"
     CDS             4331..5626
                     /locus_tag="VMZ53_17010"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HUS30212.1"
                     /translation="MPGLPLIGNLMKFRRDRLGLQDDAAKVGPIARVQLAHIPIYVIT
                     DADIAHEVLVDNAAKFKKSAGIRFLKPLLGEGLLTAEGDVHKRHRKLLAPAFAPKRLA
                     AYGEVMVDETRRTLAHWRNGARIDLADEMMEMTLAIAGRTMFGADVRGDAEAVARGLE
                     LGMRAQTAALRNPLMLTSLMPKNKDIERSAELLDGVVYRLIREGRARGTDKGDVLSIL
                     LLARDEEDGTGLSDKQIRDEVMTLLLAGHETTANALTWTWYELARHPDVLAKLQQELQ
                     PLGNRVLTVEDLQKLPYNLAVVEEAMRLHPPAYATAREALVPVVVGGHMLPEKSVVLV
                     NIRGMHRRADYFPAPDAFMPERMLADAKKGRQRNYYLPFGAGPRVCIGSHFALLEAQL
                     ALATMVQHTRLRLLSTNVVPEPLVTLRPLGGMPAVVELR"
     gene            complement(5618..6013)
                     /locus_tag="VMZ53_17015"
     CDS             complement(5618..6013)
                     /locus_tag="VMZ53_17015"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HUS30213.1"
                     /translation="MKPIVLYDGTCGLCHKSVKFILKNERDHEIQFAPLQGPTAAELR
                     TKHPEIPDNVDTVVLVENNRARLRSKAFMYISHHLRAPWRWGYAFRWMPAFLLDLGYR
                     FIAAIRYRIWGRADACDLPSPEHRIRFLP"
     gene            6060..7220
                     /locus_tag="VMZ53_17020"
     CDS             6060..7220
                     /locus_tag="VMZ53_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUS30214.1"
                     /translation="MLRILLGALVSGSIHFYVWWRVVRRARLPRRVHWAATILMLVLW
                     LAIPITTTARLWSPQLSKSLAWISMPWMALCGLLFVASVALHIATTLGWLGRIALRRP
                     PPSQDGMSRRVFLARVTSATAVTVASASVARGLTQARGVHEIVDVEVKLAKLPKALDG
                     FSIVQLSDLHVGMTIDRAFVQKVVDLTNGLKPDLVALTGDLIDGPVKELADDVAPLAD
                     LRAKHGVFAITGNHEYYAGADPWIAHLSSLGARYLRNELVTIGEGDAAFDLAGVDDHS
                     SRSYAGHGEDLAAATAKRDPSRALVLLAHQPRQVRRAQKYGVDLQLSGHTHGGQIWPW
                     HYLVKIQQGGLLAGRYQFGDTQLYVTRGCGYWGPPVRVLAPLEITRVILRSA"
     gene            7217..>7438
                     /locus_tag="VMZ53_17025"
     CDS             7217..>7438
                     /locus_tag="VMZ53_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30215.1"
                     /translation="MRSAALVLMLVACGRHHEPGTLDDKAAKELFDVVELKDVAPGQS
                     DLTLDEKGTIWMVSERARVVVELTTPPVKH"
BASE COUNT         1283 a         2453 c         2452 g         1250 t
ORIGIN      
        1 tgtcgggatg cacgagcttg atcgcggtcg gacgtcgcag catcgcgtga tgcgcgcgat
       61 aaaccgcacc gttgccgccc tcgccgatct ttcgatcgag cgtgtactgg ccgaggcgca
      121 tcgcggcgct cgcctgcttg cgcaatccgt agatgacgtt cgagccgatt gtgacgagga
      181 cgatcaccgc gcagctaatg acgagcgcgc ttccgacgta cgcatcggcg gggagctcga
      241 tgtgtttatt gcgccacaac gcttggccga cgccggcgag gttcagcgga agaaataaca
      301 acacgccggc gatcgccgtc cgccgtcctg tgctcggcac tacgatcgcg cgcaagaaga
      361 tcatgaaaca ggaccacagc atgttcgcga acgcggacga ctgcagatca gaggcgatga
      421 acgtcgaggc tccgaaaatg atcccggtcg cgagcgcata aaacaggtcg atcgcgtaga
      481 gccactcgag cctgagctgg cggcgtacga ggaagccccg ccagatgatc gcctcgatga
      541 cgagaccgac cgtcgcgatc aaatagatca ggttgttgtc tactggctcg atcagcgggt
      601 agcgccaata cagaaccttc atcccgccga gcagcgcgat gaagctccag aacgcgagct
      661 tcgaaagcac cgtcaagcgc gcttgcaggt atgcccgcga ctcctcatcc cgcgcggagc
      721 catgccagcc cacacgcgtg atcatcttcc tagagccagc cggcccttca cccattccgc
      781 gacggtaaca tcactccgct gctggttcga acgcgaggcg caaaccggac ttgatcgcct
      841 tcttcggcgt gccggcgaac cacgtgactt cctgttcggc ggcgttcagg agcgggacgc
      901 gctgcgcgag caggtcgtcg cagaacccag ccatatgccg atcgccgatt cgctgactga
      961 cctcgcgcag caggcgcgcc gtcgcaatgc cgtgatggaa gccgagcagc gtgcctcgat
     1021 agctgcgctc gacgtcgatc attcgatcga acacgaggtg ccgcagcgtc gagaaaatct
     1081 gaccgaccgt gcgggcagcg accacaccga tcggctgccg agtcgtcatc agcgcactca
     1141 tccgcggccg cagcgacgca gcgtgcgcac cgagtgagcg caatgccgcc gctgggggaa
     1201 catcgccgag gcggcgagct tcgcgacgag tatgaatcat ggccgaatgc tcgctctgcg
     1261 cgagctcgcg gcacagcttt gcgtagagct tatgcagtgg cgtcacgact actggaagtg
     1321 cgagctacgt gccgcattct cgacgagaaa ctccgacgga ccacggcgca cgcgttgcag
     1381 cacattcgcc tatgcgcaga aaaccggtga aacgcgtgaa acgcatgctc tggtcggctg
     1441 gcttgacgat gggcttcggt gcgctcgtcg cggcggtgat ccagcgctac cgccagctcc
     1501 ccgcgaacga cacatttgca gatgacgaga gcggggtaga ctccggcaag cgggagacgg
     1561 tcatgcaaca caacatcgca gtgaacgtcg gcgaccaggt gtttctcgag gagggtggcg
     1621 aggaaatcgg cgccgtccgc aaggtcgagc gcgaccacct cgtcgtctac atcgaggcag
     1681 ccggagactt cgtgatccgc ggccccgaga tcaccgccgt ccatcacggc aaagtgatcc
     1741 tcgcgcccga caaggtcgag ggacgcctcc tcgacgcggc ccgcggcgcg cacgaacgcg
     1801 aagtgttcta gaacgcgcgc gaaaccgcgc gggtgtgcgc gggcgccctg ccccgacggc
     1861 tcaacctatg aaaaggcgaa gggcctgagg tgcatgcggt ttgcacagta ggcgcgctgc
     1921 gtgacccaac attcggcgac gatgcggctc ctcgacgagg agacccgcga ccttcacgcc
     1981 gatgtcgacg cctactggct ggatctcatg gcgtcgggag taaccaagga ctcctaccga
     2041 gcgcagctga ttcgggtctt cggcttcgag gcgccattcg agagcgcgct cgcgtacacg
     2101 ccgcacctga tcgtccccga ccgccgcgac ctcacgcgct cgggcctgat cgcgcaggac
     2161 ctgctcgcgc tcggcattac gccggccaag atcacggcgc tgccgcagtg cccggagatc
     2221 gcgccgttcg aagatccggc cgaggcgctc ggctggaagt acgtcgccga acgtccgacg
     2281 cagctgcact cggcgatcaa gcgcaacatc gtgtcgcgcg tcgccgacag cgcaaacgcg
     2341 ctcgcatatc tctcggcgta cgacggcgtg gccgctgcgc gctggcaaca gctcggcgtc
     2401 ttgctcgaga atgtggcggc gcggccgggc gcgcagtcaa agctcgtcaa agcggcgaag
     2461 accgcgttca cgtgcatggg cgcgtggttc cgcgcgcaca agaatcacga ccacgctgcg
     2521 ctctgactag cgctgctcgt cgcacgcaga agagtcacga ccgcgctgcg cgtgcggtaa
     2581 gcgccgctat ggcgcgtcgc agatcgggcc gaggtcgtcg accgtctccc acgcggtttc
     2641 gggaaccgtc atccacgaga cgtggttgcc gagcggcgca ctgccttgcg cgacatcgta
     2701 ggtcgcaaac tcgccgggac cttcggtgag cagcactctc gcgccggtgt tgtcgacgag
     2761 cgttgcgagc agcgtgagtg ggtggctctg gccttcggag atcttgatcg cgccgctgcc
     2821 actcgggacg aacacgccac tccagcgctc gttcggccag ctgcattgat ccgccggcga
     2881 gccgacttgc agccagttga ccgcgccggt ccacgcgacg gtggcgccgt tcactttcac
     2941 ttcggcgaag gcccacagcg tttcgcatgc gggcggcgtg cacgtgtgcg gcggaacgat
     3001 cgcggcgatc gccggcggca gcggcgtttc ctttgcgggg cgctgccact gcttcgcctt
     3061 gtccatttcg ccgaggcgcg cggcgaccgg ttgcggcggc tcgaagcggc agcccgtcgg
     3121 tgcggcatcg gagacgagca cgaagccgcc agcctcgtcg ggatttgcgg gcgcggggca
     3181 gccttcgatc tcgacgccga acgtcgcgct cgcgggcggg ccatcgatac gcgcttggta
     3241 gtagcggccg agcttcaccg ggcacggcgg cgccgtttgc gtgacgagcc agagtgcgcc
     3301 ttgtggggcg gccggaacgc cggctgcgct cgcagcttgc gcgtcgagct gggcgtactc
     3361 gggaacctgc gaagcatctt gtgcggcctg cgcgtgcagc gggatcaccc agccgctgcg
     3421 gcccttgccg gggtcttgct gcacgaacga tgcgacgagc acgttcttcg ccgatgggca
     3481 gcctgcacct tgcgctgtcg gaccggtgcc cgtgcccttc ttcgcgggac acgcagcgag
     3541 cacgagcagg agtaacgcgc gtttcatgcg cgcgacctta gctcaattcg cggcgaggcc
     3601 gaggtagcgc gtgacgagca gggtcgtctc gtcgacgagg cggggatcgc gaagacgcgc
     3661 gggatagagc agcgctgcgc gatgcaccac ggcttcgatc gcagagacga ggatgaacgc
     3721 ggcgagatcc ggatcgcgaa tcgccagctc gtctttgcgc gcggcgagca gacccgcgac
     3781 catgcgatga cagatttcat cgagctcgcg aaggcgagcg agcttgccga tgcgcggcac
     3841 ttgctccgtc agcacgcgat gcagctccgg gtcgatcgag tgcgcgcgga tcgtcagctc
     3901 gaccacggcg cgtgcggcct cggcaagcgg cagcttcgcg acgcgcgtga gctccgcgag
     3961 gattgccgcg ttcatgtctt ccatgtggcg atcgatgagc gcggagacca gcgcctcttt
     4021 gttcgggaag tattgataga gcgagccgat cgacactccc gcctgcgcgg ccacagcgtt
     4081 ggtcgacagg ccatcgaagc cctgcttcac cagaacccga gtcgttgctt cgaggatcgt
     4141 gtcgaccgtc gccttgcttc gctcttggcg cgggcgtttt cgtggcgtgg tccggacttg
     4201 gcgctgcatc gagggagcct ttcgtcgctc gcggaacgcg agtagacaat gtgagcgatt
     4261 gctcgcattt tagacatcaa tggcaacgtt gaccagcacg ttcggatccg ccgttcgttc
     4321 catcccgccg gtgccggggc tcccgctcat cggcaacctc atgaagttcc gacgagaccg
     4381 cctcggcctg caggacgatg ccgcgaaggt cggaccgatc gcgcgcgttc agctcgccca
     4441 catcccgatc tatgtgatca cggacgcgga catcgcgcac gaggtgctcg tcgacaatgc
     4501 ggcgaagttc aagaagtcgg ccggcattcg ctttttgaag ccgctgctcg gcgaaggcct
     4561 cctgaccgcg gaaggcgacg tccacaagcg acatcgcaag ctgctcgcac cggcgttcgc
     4621 accgaagcga ctggccgcgt acggcgaagt gatggtcgac gagacgcggc gaacgctggc
     4681 tcactggcgc aacggcgctc gcatcgatct cgccgacgag atgatggaaa tgacgctcgc
     4741 gattgctggc cggacgatgt tcggcgccga cgtgcgcggc gatgctgaag cagtggcgcg
     4801 aggcctcgag ctcggcatgc gcgcgcagac cgccgcgctt cgcaatccgc tgatgctcac
     4861 gtcactgatg ccgaagaaca aagatatcga gcgctccgcc gaattgctcg acggcgttgt
     4921 ctatcgcctg atccgcgaag gccgtgcacg cggcaccgat aaaggcgacg tgttgtcgat
     4981 cctcttgctc gcgcgcgacg aagaggatgg cacggggctg tccgacaagc agatccgcga
     5041 tgaggtcatg acgcttctcc tcgcgggtca cgagacgacc gcgaacgcgc tgacgtggac
     5101 ctggtacgag ctcgcgcgtc atcccgatgt cctcgcgaag ctgcaacaag agctgcagcc
     5161 gctcggcaat cgcgtgctca ccgtcgagga tctgcagaag cttccgtaca acctcgcggt
     5221 cgtcgaagag gcgatgcgcc tgcatcctcc ggcgtacgcg accgcgcgcg aggcgctcgt
     5281 gcccgtggtc gtcggcggcc acatgctgcc cgaaaaatcc gtcgtgctcg tcaacatccg
     5341 cggcatgcat cgccgcgccg attacttccc cgcgcccgac gcgttcatgc ccgagcgcat
     5401 gctcgccgac gcgaagaagg gccggcagcg caactactac ctgccgttcg gagcgggccc
     5461 tcgcgtttgc atcggctcgc acttcgcgtt gctcgaggca cagctcgcgc tcgcgacgat
     5521 ggtgcagcac acgcggctgc gtctgctctc cacgaacgtg gtgcccgagc cgctcgtcac
     5581 gctgcgtccg ctcggcggga tgcctgcggt cgtcgagcta cggtaggaag cgaatgcgat
     5641 gctcggggct cggcaagtca cacgcgtcgg cgcgtcccca gatccgatag cggatcgcgg
     5701 cgatgaatcg gtacccgaga tcgagcaaga acgccggcat ccagcgaaac gcgtaccccc
     5761 aacgccaagg agcgcgcagg tgatgcgaga tgtacatgaa cgccttcgag cgcagccgcg
     5821 cgcggttgtt ctcgacgagg acgacggtgt cgacgttgtc gggaatctcg ggatgcttcg
     5881 tccgcagctc ggcggccgtg ggtccctgca gcggcgcgaa ctggatctcg tgatcgcgct
     5941 cgttcttcag gatgaacttg acgctcttat gacagagccc gcatgtcccg tcgtacagga
     6001 caatcggttt cacgggctcc atatccgcat catgccgcgt cctgctagcc tggcaaggcg
     6061 tgcttcggat ccttctcggt gcgctcgtct cgggctcgat ccacttctat gtgtggtggc
     6121 gcgtcgtccg tcgcgcgcgg ctgccacggc gggtgcactg ggctgcgacg attttgatgc
     6181 tcgttctgtg gctcgcgatt ccgatcacga cgaccgcacg gctgtggtcg ccgcagctct
     6241 cgaaatcgct cgcatggatc tcgatgcctt ggatggcact gtgcggcctg ctgtttgtcg
     6301 cgagcgtcgc gctccacatc gcaaccacgc tcggctggct cgggcgcatc gcgctgcgtc
     6361 gccctccccc gtctcaggac ggcatgtcga ggcgcgtctt cctcgcgcgc gtgacgagcg
     6421 ctacggcggt caccgtggct agcgcgagtg tcgctcgtgg gctcacgcag gcgcgtggcg
     6481 tgcacgagat cgtcgacgtc gaggtgaagc tcgcgaagct gccgaaggcg ctcgacgggt
     6541 tctcgatcgt gcagctctcc gacttgcacg tcggcatgac gatcgatcgc gcgttcgtgc
     6601 agaaggtcgt cgacctgacg aacgggctga agcccgacct cgtcgcgctc accggcgatc
     6661 tcatcgacgg ccccgtgaag gagctcgccg atgatgtcgc accgcttgca gacctgcgcg
     6721 cgaagcacgg cgtgttcgcg atcaccggca accacgagta ttacgccggc gccgatccat
     6781 ggatcgcgca tctcagctcc ctcggcgccc gctacctgcg caacgagctc gtcacgatcg
     6841 gcgaaggcga cgcggcattc gatctcgccg gcgtcgacga tcactcgagc cgcagctacg
     6901 caggccacgg cgaagacctc gcagcggcga cggcgaagcg cgatccatcg cgtgcgctcg
     6961 tgctgctcgc gcatcagcct cgccaggtgc gacgcgcgca gaagtacggc gtcgacctcc
     7021 agctctcggg tcacacgcac ggcggccaga tttggccgtg gcactacctc gtgaagatcc
     7081 agcaaggcgg cctgctcgcc ggtcgctacc aattcggcga cacgcagctc tacgtgacgc
     7141 gcggctgtgg ctactggggc ccgcccgtgc gcgtgctcgc gccactcgag atcacgcgcg
     7201 tgatactacg aagcgcatga gatcggccgc gctcgtcctg atgctcgtcg cgtgcggcag
     7261 gcatcacgag ccgggcacgc tcgacgataa ggccgcgaag gagctgttcg acgtcgtcga
     7321 gctgaaggac gtcgcgcccg gccaatcgga tctcacgctc gacgagaagg gcacgatctg
     7381 gatggtgtcc gagcgcgcgc gcgttgtcgt cgagctgacc acgcccccgg tgaagcat
//
LOCUS       DATLBZ010000324         4473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1860313, whole genome shotgun sequence.
ACCESSION   DATLBZ010000324 DATLBZ010000000
VERSION     DATLBZ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860313"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VMZ53_17030"
     CDS             complement(<1..321)
                     /locus_tag="VMZ53_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30216.1"
                     /translation="MAAARAPRAAHELRTEPRTEARGSFNIITVAYALGALLVLFALG
                     WFLAERGSKLGPAGVLLVAGLYAAAFARAGATLRRRGFPIAGGLAIVLAVTMTPVWGW
                     AVSRL"
     gene            530..1381
                     /locus_tag="VMZ53_17035"
     CDS             530..1381
                     /locus_tag="VMZ53_17035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HUS30217.1"
                     /translation="MASIWKGSLTFGLVHVPVEMKTAVRSDHVSFRMLHAEDMSPIKY
                     ERVCAVDGEPVPWNEIVKGYEYEKGKYVVLTDEDFKEAALESSRTIDILDFVKEDEID
                     PRFFETPYYLLPTKGGDKPYALLREAMRQSGVIGVGKVILRRSQHLAGVRVVGQALVI
                     ELMRFKNELVDISEFSFPGTDAIREQEMSMATQLVENLAEPFDPEKYTDDYRANLMKL
                     IRAKMKGKKVTAAEPLPDDEDAKVLDLMARLQASLEEAKAGGEKKRVAKTPRKSGGGR
                     SRQKKTA"
     gene            1405..4194
                     /gene="ligD"
                     /locus_tag="VMZ53_17040"
     CDS             1405..4194
                     /gene="ligD"
                     /locus_tag="VMZ53_17040"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HUS30218.1"
                     /translation="MATRKQAPRSRGANRPLGEYNAKRDFTRTDEPAGDVPRPSRAKA
                     LRFVIQKHAASHLHYDLRLELDGVMKSWAVPKGPSLDPADRRLAVEVEDHPVEYNTFE
                     GTIPAGEYGGGTVMLWDRGTYEPDSGDVDDLRRGHERGDIKFTLHGERLRGSFALVRM
                     RGRGGSKPQWLVIKHRDADAVPGWDITTVDTSVATGRTMDEIAGGKRVWSSNRKATAK
                     KSTAKKKTTAAKSATATRSRRSDSRFPIPDSRISPMLATIGTEIPRGEGWTFEPKYDG
                     VRVLAFASGDDVRLITRNGLDKREQFPEVTAGVRALAKRRKRPFVLDGEITAFNGDSP
                     ARFQALQNRMHLKDASDISGHVKTTPSALVAFDLLVDGDDVLVAEPWTTRRKRLEKLI
                     GTSAITGVRLGDTGPGDGEKMLARARRDDWEGIMAKRTDATYQPGARSRDWLKLKVEF
                     RQEFVVGGFTEPRNTREHMGAMLLGHYDGDDFVYVGHTGGGFTRESLAAMAKRLKRLE
                     RKSSPFSTKVRTNEQAHWVRPEIVVEVKFNEWTDDDRLRQPIFLGVRDDKAAREVTRE
                     GTSVQRAKRGGTREAATRRRVGAAAMPAKKRSTTTKNASASGDNRLETELSEAEQAGR
                     DAHLTLGRGVSLKLSSLDKVWFPGRAGGFTKGDVLRHYVRVADLILPVMKDRPIVLKR
                     FPDGIDGQAFYQQKAPQNPPEGVRVETIEDADGDCVERVIGGSLATLLYQVQLGTISV
                     DPWHARVQALGFADYSVIDLDPGPRAKFERVVEVATWVKEELDELGLHAAIKTSGSTG
                     VHIFIPLPPKTSNESALLIAQIVATRVALAHPKEATIERSLAERSKSSVYVDYLQNVL
                     GKSVAAAYAVRARPGATVSTPLEWSELNAKLDPRDFTIETVPARFAKTGDLWAPALKR
                     ANTAAAIKGLTTKAR"
BASE COUNT          866 a         1404 c         1522 g          681 t
ORIGIN      
        1 gagtctcgag acggcccaac cccagaccgg cgtcatggtg accgcgagca cgatcgcgag
       61 cccgccggcg atcgggaatc cgcgacggcg caatgtcgcg ccggctctgg caaacgcggc
      121 ggcatacagc cccgcgacca gcagcacgcc ggccggcccg agcttcgacc cgcgttcggc
      181 gaggaaccag cctaacgcga agaggacgag gagggcgccc agtgcgtagg cgacggtgat
      241 gatgttgaag ctgccgcgcg cctcggtacg tggctcggtg cggagctcgt gcgcggcacg
      301 gggcgcgcgt gcggcggcca tctcatctgc gagagcgttc agctggtcgc gttgcggcgc
      361 ggtgatgatg ccgcgctcga cggccgtgtc ggtcagcgtg ctcagcggcg gtgcgtgttc
      421 ggacgatgtt cgcatatatc acatttctgg ctttccacac ccggcattcg acttgctttc
      481 attcgcgaac gcgacgacga gcaccatcac cctcaaccat aggccaacga tggcgtcgat
      541 ttggaaagga tccctgacgt tcggcttggt ccacgttcca gtcgagatga agacggcggt
      601 ccgaagcgat cacgtcagtt tccgaatgtt acacgccgaa gacatgtcgc ccatcaaata
      661 cgagcgcgtg tgtgcggtcg acggcgagcc agtgccctgg aacgagatcg tgaagggcta
      721 cgagtacgag aagggcaagt acgtcgtgct cacggacgag gacttcaagg aagctgcgct
      781 cgagtcgtcg cgcacgatcg acattctcga cttcgtgaaa gaggacgaga tcgacccgcg
      841 cttcttcgag accccgtact acctgctgcc gacgaaaggc ggcgacaagc cctacgcgct
      901 cctgcgcgag gcaatgcggc agagcggcgt gatcggggtc ggcaaagtga tcctccgtcg
      961 gtcgcagcac ttggcaggcg tgcgcgtggt cggccaggcg ctcgtcatcg agctcatgcg
     1021 attcaagaac gagctcgtag acatcagcga attcagcttc cccggcaccg acgcgattcg
     1081 cgagcaggag atgtccatgg cgacgcagct cgtcgagaac ctcgcggagc cgttcgatcc
     1141 cgagaagtac accgacgact atcgcgcgaa cctcatgaag ctcattcgcg cgaagatgaa
     1201 gggaaagaag gtcacagcgg cagagccttt gcctgacgat gaggacgcaa aggtgctgga
     1261 cctgatggct cggcttcagg cgagcctcga agaggcgaaa gctggcggcg agaagaaacg
     1321 cgtcgcgaag acgccgcgaa agtccggcgg cggacgaagc cggcagaaga agaccgccta
     1381 acggagcagg aggaggcagc gtcagtggcg acacggaagc aagcgccgcg ttcgcgcggc
     1441 gccaatcgcc cgctgggcga gtacaacgcg aagcgcgact tcacgcgcac cgacgagccg
     1501 gcgggcgacg ttccgcggcc gtcgcgcgcg aaggcgctgc gcttcgtgat tcagaagcac
     1561 gccgcgagtc acctccacta cgatctacga ctcgagctcg atggggtgat gaagagctgg
     1621 gcggtgccga agggcccatc actcgatccc gccgaccggc gcctcgccgt cgaggtcgag
     1681 gaccatcccg tcgaatacaa cacgttcgag ggtacgatcc ccgccggcga gtacggtggc
     1741 ggaacggtga tgctctggga tcgcggcacg tacgagcccg attcgggcga cgtcgatgac
     1801 ctgcgtcgcg gccacgagcg tggcgacatc aagttcacgc tgcacggcga acggctgcgt
     1861 ggatcgttcg cgctggtgcg catgcggggc cgaggtgggt cgaagccgca gtggctggtg
     1921 atcaagcatc gcgacgcgga tgcggtgccg ggctgggaca tcacgacggt cgatacgtcg
     1981 gtggcaacgg ggcgaacgat ggacgagatc gcgggcggca agcgtgtgtg gtcgtcgaac
     2041 cgcaaggcga cggcgaagaa gtcgacagcg aagaagaaga ccaccgccgc aaagtcggca
     2101 accgcgacgc gcagtcgccg ttccgattcc cgattcccga ttcccgattc gcgaatttcg
     2161 ccaatgctcg ccaccatcgg caccgagatc cctcgtggcg agggctggac gttcgagccc
     2221 aagtacgacg gcgtgcgcgt gctcgcgttc gcgtcaggcg acgacgttag gctcatcacg
     2281 cggaacggtc tcgacaagag agagcagttc cccgaagtca cggccggcgt tcgcgcactc
     2341 gcgaagcgac gcaagcggcc gttcgtgctc gatggcgaga tcaccgcgtt caatggcgac
     2401 tcgcccgcgc gatttcaggc actccagaac cgcatgcatc tgaaagacgc gtcggacatc
     2461 agcggccacg tcaagaccac gccgtccgcg ctcgtggcgt tcgacctgct cgtcgatggc
     2521 gacgacgtgc tcgtcgcgga gccctggacg acgcggcgaa agcgtctcga aaagctcatc
     2581 ggtacgagcg cgatcaccgg cgttaggctc ggcgacacgg gcccgggcga cggcgagaag
     2641 atgctcgcac gcgcccggcg cgatgactgg gaaggcatca tggcgaagcg cacggacgcg
     2701 acctaccagc ccggggcgcg ctcgcgcgac tggctcaagc tgaaagtgga gtttcgccag
     2761 gagttcgtcg tcggcgggtt caccgagccg cgcaacacgc gcgagcacat gggcgcgatg
     2821 ctgctcggcc actacgacgg cgacgatttc gtgtacgtcg gccacactgg cggcggcttc
     2881 acgcgcgaga gcctcgccgc aatggcgaag cggctcaagc gcctcgagcg aaagtcgtcg
     2941 ccgttctcga cgaaggtccg cacgaacgag caggcgcact gggtgaggcc cgagattgtc
     3001 gtcgaagtga agttcaacga atggaccgac gacgaccgct tgcgccaacc gatcttcctc
     3061 ggcgtgcgcg acgacaaggc cgcgcgcgag gtgacacgcg agggcacgag cgtccagcgc
     3121 gcgaagcgtg gcggcactcg tgaggcagcc acgcgtcggc gcgtcggcgc ggcggcgatg
     3181 cctgcgaaga aacggtcgac gacgacgaag aacgcctcgg cgtcggggga caatcggctc
     3241 gagaccgagc tgtcagaggc ggaacaggct gggcgtgatg cgcacctgac tcttggacgc
     3301 ggtgtgtcgc tcaagctgtc gagcctcgac aaggtgtggt ttcccggccg cgccggcggg
     3361 ttcacgaagg gtgacgtcct gcgacactac gtgcgtgtcg ccgatctcat cctcccggtc
     3421 atgaaggacc ggccgatcgt gctcaagcgc ttcccggacg gcatcgacgg tcaagcattc
     3481 tatcagcaga aggcgccgca gaacccacct gaaggcgtgc gtgtcgagac catcgaggat
     3541 gcggacggcg attgcgtcga gcgtgtcatc ggcggctcac tcgcgacgct gctgtatcag
     3601 gtgcaactcg ggacgatctc cgtcgatccg tggcacgcgc gtgtgcaggc gctcgggttc
     3661 gcggactact cggtgatcga tctcgacccc ggaccgcgtg cgaagttcga gcgcgtcgtg
     3721 gaggtcgcga cgtgggtgaa ggaggagctc gacgagctcg ggcttcacgc ggcgatcaag
     3781 acgtccggat ctacaggggt gcacatcttc attcctcttc cgccgaagac gagcaacgag
     3841 tcggcgctgc tcatcgcgca gatcgtcgcg acccgcgtcg cgctcgcgca tccgaaggag
     3901 gcgacgatcg agcgctcgct cgcggagcgg tcgaagtcga gcgtgtacgt cgactacctg
     3961 cagaacgtgc tcggcaagtc ggtcgcggcg gcgtacgcgg tccgcgcgcg acccggcgcg
     4021 acggtttcga cgccgctcga gtggtccgag ctgaacgcga agctcgaccc gcgtgacttc
     4081 acgatcgaaa cggtgccggc acggttcgcg aagactggcg atctctgggc gccggctctc
     4141 aagcgggcaa acacggcggc ggcgatcaag ggcctaacga cgaaggctcg ctgaatttca
     4201 ggggtcaggt tcgttgaatt cgagatgctc ggatgcacac agctcccgga attcaacgaa
     4261 cctgacccct tgaaattccg cgaggaatcc ttggctcgaa ccggctatcg cagccgatcc
     4321 acgtagctgt ctcctgtgat catcctgacg acgcggccgt tctcctccac gaaccgaaca
     4381 acctcgccca caggaccacc gccactcggc gcgacatagc ggaacaagcc tccaccgagc
     4441 ggctcgagcg tgaccggatt gtccacgttc gtc
//
LOCUS       DATLBZ010000325         7066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1860872, whole genome shotgun sequence.
ACCESSION   DATLBZ010000325 DATLBZ010000000
VERSION     DATLBZ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860872"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..877
                     /locus_tag="VMZ53_17045"
     CDS             68..877
                     /locus_tag="VMZ53_17045"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HUS30219.1"
                     /translation="MRDLLEVAKRAASTASDLLRNAHAEDIRSKSNQFDLVTEWDLRS
                     EEAIRRVLADTHIPVLGEEMGTLSPSSTPAGDLRWVVDPIDGTVNFSHGLPIWSISIA
                     LERVLPDGSPTRGESLLGVVAAPALGWSVSAIRGEGAFDDRGTPLRVSSRAQLSKSML
                     ATGFPYDAATNPANNFAEWDHFQRLTNACRRLGCASIDLCLVARGFYDGYWERRLSPW
                     DLAAGALFVVEAGGTVTNTQGGPFDAHSGEAAATNGAIHGELLSELARVRS"
     gene            874..2460
                     /locus_tag="VMZ53_17050"
     CDS             874..2460
                     /locus_tag="VMZ53_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HUS30220.1"
                     /translation="MKRLVAIAVVVGLFGSAAAQPAQKVPSVPAQPVPAKKVPRKAAP
                     KGPKPADPVPADPGPSDPYADPAPTDPYADPAPKVDPKKDPKTDPKKDSKADPKKDPK
                     KDPKAGKDGKPVDPYALPTVETSDKDAPPTIPVRVGLSDLPAVQGLLAVQHLDAWLLY
                     DRDAQNSIALRLVKPDGRPQRAWFYMIPAKGDPIAVVHSSEVRNFENVPGKKLTYLGY
                     RDMDKQMRAMLKGMKSIAIEYSPKAAVPNVSRVDAGTVELIKAAGVQVRSSETLVQYT
                     KAIWGDPGRTAHYVAVHHVVELRKEALAFIVKQINLRQPVTEYDVQQRIIKSMTVRGL
                     VGPPPVVAAGVNTADPFYVPTAAKTAPIKFGDLVVLGIAAKVDKPDGIYAAQTWCAVV
                     DKKVPDEIAKAFNTASLARDQALVLITDRSHKHRPVTGAEVDQTTRAFMKKAGVAANV
                     MHRTGHSIDNDLQGGGADLDDFEVKDTRIMTPGTGFTVGPGLYFNGQFGVRTEVSVYL
                     SPTGPEISTPAQDDVEALYK"
     gene            2457..3092
                     /locus_tag="VMZ53_17055"
     CDS             2457..3092
                     /locus_tag="VMZ53_17055"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HUS30221.1"
                     /translation="MTASAPTNTHVSALILAGGKATRLGGISKHEIVVEGETIFARQV
                     RVLAPRVAEIIVAGPTIEGYRNVTDVKEGAGPLAGIAAGLAACRTPWLLVVAGDMPHI
                     SAELIDALIACVSAPRTGSGTSSQETRDVDAIETIDAVGVRVNGLPEPLLCVLHTRVL
                     PTVEQALAAGRYKASRILTDDGLWVRWLEAERPAVRNINSPEDLRAERGSK"
     gene            3158..4237
                     /gene="dusB"
                     /locus_tag="VMZ53_17060"
     CDS             3158..4237
                     /gene="dusB"
                     /locus_tag="VMZ53_17060"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HUS30222.1"
                     /translation="MRVGPLQIDTPVLLAPMAAVTDLPFRTVAEELGVGFTITEFLSA
                     HALAVGDKKTSAKMTASLDGRKFGVQIFGREEDKMGEAAKMAVAIGASLVDINMGCPA
                     KRVVAGECGSALMREPAIAQRLVKAVKDAIPANIPVTVKHRAGWNQDNRNAPEFACAM
                     VEAGAAMITVHGRTRTQGFSGKSDNEIIRRVRDALPSHIPLVGNGDVIDVAGFLRMRE
                     ETGCDAVMIGRGALGNPWLFGRLREVCAGRPDPGPPTLEERVAVFRRHLHLIETLKNG
                     PRLMHEVRKACAWYARGLYGCNALRLRVWEAPDLATARALVDEYFTQLLDRKNRLGLS
                     PEGERGTKDAAFGGESADGDGEAAA"
     gene            4240..5280
                     /locus_tag="VMZ53_17065"
     CDS             4240..5280
                     /locus_tag="VMZ53_17065"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS30223.1"
                     /translation="MLRPDVRAHDFLVAESKAMRAVADQVAHYADGDAPVLICGEHGV
                     GRELVARVLHQRGPRGRGRFVAVRPTFQGADVPHVAGDDECERARRALRAAHGGTLLV
                     KDVSDLSAPSQKTLKRAIRDRKTTGTDDAADVQVVATADIDVDYAVEAQIVSRELYDL
                     FASRRIEVPPLRDRSDDLPILFDRWVKHYSAETGRTKPTVSSRAHDRLANYPWPGNVA
                     ELKSIARRLLVRVQRSRIEAGDVDEVLPVVATRVPLEDLAFEEMVKSKLAGLLARIDG
                     YPVHDLYEKVLARVERPLFDLVLAHTGGNQLKAAEILGLNRNTLRKKLADLGMEQKKK
                     KLGREDRALDEA"
     gene            complement(5287..6375)
                     /locus_tag="VMZ53_17070"
     CDS             complement(5287..6375)
                     /locus_tag="VMZ53_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30224.1"
                     /translation="MRRIGAALAVTLGVACGGSSASIDAPPSTIDAADHSWDSVRAHY
                     GTMLAVAGAGATDNSGANEWLPAFEGGPALSAELSRPHMAAADAAGNIYIADKEAHAI
                     RKVTPAGVITTVAGTGTATAVDDTPGPATQRSLGNPNGLYVRPDGTLYILDLDHGRIR
                     KVATDGTMTTLVTVTGGIAIGRGLWVTDDETVYFASGNVIKKATGTSVTTFASGFVNL
                     GNIAMRADGNLLVSDRGGNRVYLVGPTGNKTPIAGSGATGAGVDGANALATPLEGVRA
                     VWPAFVDDGGGFFAGTHEGCQLWFIDGAGVAHLFLDGAKDAHAGVGETWNTPGKKVSE
                     LRSVTVDTHGNVIIVDDDRGFVRSVQRK"
     gene            6413..>7066
                     /locus_tag="VMZ53_17075"
     CDS             6413..>7066
                     /locus_tag="VMZ53_17075"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HUS30225.1"
                     /translation="MAERPLPWISIAIGVVVGAVLAFGNCLIGLKTGLWDTAQLTSTL
                     LTFVLCAPLCRLVGKTFDQHDNNIAQTTSAAIAAMPATMGLLGAVPALGMMGSEPSTV
                     VLVVSAICFGMLGVASALLLAPRLIDRDQLPFPTGVATAEFIGALHTDRRAVRERALM
                     FGIAFAVTALFTLVRDVGEWVPASIETTIVVAAIPVVVGVSASPLLVGSGALIGARIA
                     "
BASE COUNT         1196 a         2408 c         2304 g         1158 t
ORIGIN      
        1 acgctgcgcg gaaccaacct cgtcgcgatg cgcctcgatc tctgatcggc tctgatcggc
       61 tctgggcgtg cgcgacctcc tcgaagtagc caagcgtgcg gcgagcacgg cgagcgacct
      121 gttgcggaac gcgcacgccg aagacattcg ttcgaagtcg aaccaattcg atctcgtcac
      181 ggagtgggac ctgcgctccg aagaagcgat ccggcgcgtc ttggcagata cgcacatccc
      241 cgtcctcggc gaggaaatgg gaacgctgtc gccttcgtca actccagcgg gcgacctgcg
      301 ttgggtcgtc gatccgatcg acggcacggt gaacttctcg cacggccttc cgatctggtc
      361 gatctcgatc gcgctcgagc gcgtgctgcc cgacggctcg cccacgcgcg gcgagtcgtt
      421 actcggtgtc gttgcggcgc ccgcgctcgg ttggtcggtc tctgcgattc gcggcgaagg
      481 tgcgttcgat gatcgcggca cgccgctccg ggtgagctcg cgggcgcagc tctcgaagtc
      541 gatgctcgcg accggcttcc cctacgacgc ggcgacgaat ccagcgaaca actttgccga
      601 gtgggaccac ttccagcggc tcacgaatgc atgccggcgc ctcggctgcg catcgatcga
      661 tctttgcctg gtcgcccgcg gcttctacga cggctactgg gagcgccgcc tatctccgtg
      721 ggatctcgcg gcgggcgcgc tgttcgtcgt cgaggccggc ggcaccgtga ccaacacaca
      781 gggcggtcca ttcgacgctc actcgggaga agcagcggcg acgaacggtg ctattcatgg
      841 ggagctcctt tccgagctcg ccagggtgcg ctcatgaaac gactcgtcgc gatcgctgtc
      901 gttgttggac tcttcggttc cgccgccgcg cagccggcgc agaaagtccc gagcgtgccg
      961 gcgcagccgg tgcccgcgaa gaaggtgccg cgcaaagccg cgccgaaggg cccgaagcct
     1021 gctgatccgg tgcccgcgga cccggggccc tcggatccgt acgccgatcc cgcgccgacg
     1081 gacccctacg ccgatccggc gcccaaggtc gacccgaaga aggatccgaa gaccgacccg
     1141 aagaaagatt cgaaagccga cccgaagaaa gatccgaaga aggatccgaa ggcgggcaaa
     1201 gacggcaagc ccgtcgatcc gtacgcgctg cccaccgtcg agacgtccga caaagatgcg
     1261 ccgccgacga ttccggtgcg cgtcggtctc tccgacctcc cggccgtgca aggcctgctc
     1321 gcagtccagc acctcgatgc atggctgctc tacgatcgcg acgcgcaaaa ctcgatcgcg
     1381 cttcgcctcg tgaagcccga cggccgcccg cagcgcgcgt ggttctacat gatccccgcc
     1441 aaaggcgacc cgatcgcggt cgtgcacagc tccgaagtcc gcaacttcga gaacgtgccg
     1501 ggcaagaagc tcacgtacct cggctaccgc gacatggaca agcagatgcg cgccatgctg
     1561 aagggcatga agtcgatcgc gatcgagtac tccccgaagg ccgcggtgcc caacgtctcg
     1621 cgcgtcgacg caggcaccgt cgagctgatc aaagcggcgg gcgtccaagt gcgctcgagc
     1681 gagacgctcg tccaatacac gaaagcgatc tggggtgacc cgggccgcac ggctcactac
     1741 gtcgcggtcc accacgttgt cgagctgcgc aaagaagcgc tcgcgttcat cgtcaagcag
     1801 atcaacttgc gccagccggt caccgagtac gatgtccagc agcgcatcat caagtcgatg
     1861 acggtgcgcg gcctcgtcgg tccaccgccg gtcgtcgcgg caggcgtcaa cacggccgac
     1921 ccgttctacg tgccaaccgc cgccaagacc gcgccgatca agttcggcga cctcgtcgtc
     1981 ctcggcatcg ccgcgaaggt cgacaagccc gatggcatct acgccgcgca gacgtggtgt
     2041 gccgtcgtcg acaagaaagt ccccgacgaa atcgcgaagg cgttcaacac cgcgtcgctc
     2101 gcgcgcgacc aagcgctcgt cctcatcacg gatcgctcgc acaagcatcg tcccgtcacg
     2161 ggcgccgaag tcgaccaaac cacgcgcgcg ttcatgaaga aggcaggcgt cgcggcgaac
     2221 gtcatgcacc gtaccggcca ttcgatcgac aacgatctgc aaggcggcgg cgcagacctc
     2281 gacgacttcg aggtgaaaga cacgcgcatc atgacgccgg gcaccggctt cacggtcggc
     2341 ccgggtctct acttcaacgg tcagttcggc gttcgcacag aagtcagcgt gtacctctcg
     2401 ccgacgggcc ccgaaatctc gacgcctgcg caagacgacg tcgaagctct ctacaagtga
     2461 ctgctagcgc gccgaccaac acacacgtca gcgcgctgat tctggccggc ggcaaagcaa
     2521 cgcgcctcgg cggtatctca aagcacgaga tcgtcgtcga gggcgagaca atcttcgcgc
     2581 gccaggtccg cgtgcttgcg ccgcgcgtgg ccgaaatcat cgtcgcgggc ccgacgatcg
     2641 aaggctatcg caacgtcacg gacgtcaaag aaggcgccgg gcccctcgcc ggcatcgccg
     2701 cgggcctcgc cgcgtgccgc acgccgtggc tgctcgtcgt agcaggcgac atgccgcaca
     2761 tcagcgccga gctcatcgac gcgctcatcg cgtgcgtatc cgcgccacgg acggggtcag
     2821 gcacaagttc acaagaaacg cgtgacgtag atgcgatcga gacgattgac gcggtgggcg
     2881 tgcgcgtgaa cggcttgccc gagccgctgt tgtgcgtgtt gcacacgcgt gtgttgccga
     2941 ctgtcgagca ggcattggcg gcgggccgct acaaggcgag ccgcatcctc acggacgacg
     3001 ggctgtgggt ccgctggctc gaagccgaac gcccggcggt tcgcaacatc aattcgcccg
     3061 aggatctacg agcagagcgc gggagtaagt agcggcgacg tttgtgcttt ttgtgtcgtt
     3121 tactttttcg ccccgcatcc tgtaaatacc tcggaccatg cgcgtcgggc ccctccagat
     3181 cgacacaccg gtgctgctcg cgccgatggc ggcggtcacc gatctgccgt tccgtacggt
     3241 cgctgaagag ctcggcgtgg gcttcacgat caccgagttt ctctcggcgc acgcgctcgc
     3301 ggtcggcgac aagaagacca gcgcgaagat gacggcatcg ctcgatggcc ggaagttcgg
     3361 cgtccagatc tttggccgcg aggaagacaa gatgggcgag gccgcgaaga tggcggtcgc
     3421 catcggcgcg tcgcttgtcg acatcaacat gggctgcccg gcgaagcgcg tcgtggcggg
     3481 cgagtgcggg tccgcgctca tgcgtgagcc tgcgatcgcg caacgcctcg tgaaggccgt
     3541 caaggacgca attcccgcga acatcccggt cacggtgaag caccgcgcgg gctggaacca
     3601 ggacaaccgc aacgcgcccg agttcgcatg cgcgatggtc gaggccggcg ccgcgatgat
     3661 cacggtgcac ggccgcacgc gcacgcaagg cttctccggc aagagcgaca acgagatcat
     3721 ccgccgcgtc cgtgacgcgc tgccgtcgca catcccgctc gtcggcaacg gcgatgtcat
     3781 cgacgtggcc ggcttcctac gcatgcgcga agagacgggc tgcgatgccg tgatgatcgg
     3841 ccgcggcgcg ctcggcaatc catggctgtt cggccgcctc cgtgaagtct gcgcaggtcg
     3901 tccggatccc ggaccgccga ctctcgaaga gcgcgtcgcc gtgttccgcc gccacctcca
     3961 tctgatcgag acgctcaaga acggcccgcg cctcatgcac gaagtccgca aggcatgcgc
     4021 gtggtacgca cgcggcctct acggctgcaa cgctcttcgc ctgcgcgtct gggaggcgcc
     4081 ggatctcgcg accgcgcgcg cgctcgtcga cgagtacttc acgcaattgc tcgaccgcaa
     4141 aaaccgcctg ggcctctcgc ccgaaggcga gcgcggtacg aaggacgcag cctttggtgg
     4201 tgaatcggcg gatggtgacg gtgaggcagc agcctaacga tgttgagacc tgacgttcga
     4261 gctcacgatt ttctcgtcgc cgaatccaag gcgatgcgcg cagtggcaga ccaagtcgcg
     4321 cactacgccg acggcgatgc cccggtcctg atctgcggag agcatggcgt cggtcgcgag
     4381 ctggtcgcgc gcgtgctcca ccaacgaggc cctcgtggcc gcggccgctt cgttgctgtg
     4441 cgcccgacat tccaaggcgc cgacgtcccg cacgtcgccg gcgacgacga atgcgagcgc
     4501 gcgcgtcgcg cactgcgcgc tgcacacgga ggcacgctac tcgtcaaaga tgtcagcgac
     4561 ctctcggcgc catcccagaa gacgctgaag cgcgcgatcc gcgatcgcaa gaccacgggc
     4621 actgacgacg ccgccgacgt gcaagtcgtc gcgaccgccg acatcgatgt cgattacgcg
     4681 gtcgaggccc aaatcgtctc gcgcgagctc tacgacctgt tcgcctcccg ccgcatcgag
     4741 gtcccgccgc tgcgcgatcg cagcgatgac ctacccatcc tgttcgatcg ctgggtaaag
     4801 cactacagcg ccgaaactgg ccgcaccaag cccacggtgt cctcgcgcgc ccacgatcgg
     4861 ctcgcgaact atccctggcc gggcaacgtc gccgagctca agtcgatcgc gcgtcgccta
     4921 ctcgtccgcg tccaacgcag ccgcatcgaa gcgggcgacg tcgacgaagt gctcccagtt
     4981 gtcgccacac gcgtaccgct cgaagacctc gcattcgaag agatggtgaa gtccaagctc
     5041 gcgggcctgc tcgcgcgcat cgacggctac ccggtgcacg acctctacga gaaggtgctc
     5101 gcccgcgtcg agcgcccgct gttcgacctc gtgctcgcgc acacaggcgg caaccagctg
     5161 aaggccgccg agatcctagg cctcaaccgc aacacgcttc gcaagaagct cgcggacctc
     5221 ggcatggaac agaagaagaa gaagctcggt cgcgaagacc gcgccctcga cgaagcctaa
     5281 ccggcgttac ttgcgctgca ccgagcgcac gaagcctcga tcgtcatcga cgatgatcac
     5341 gttgccgtgc gtatcgacgg tcacgctgcg cagctcgctg accttcttgc cgggcgtgtt
     5401 ccacgtctcg ccgacgcccg cgtgcgcgtc cttcgcgccg tcgaggaaca gatgcgctac
     5461 gcccgcacca tcgatgaacc agagctggca gccctcgtgc gtgcccgcga agaagccgcc
     5521 tccatcgtcg acgaatgcgg gccacaccgc gcgcacaccc tcgagcggtg ttgcgagcgc
     5581 atttgctcca tcgacgcccg cgccggtggc gccgctgcct gcgatcggcg tcttgttgcc
     5641 cgtcgggccg acgaggtaca cgcgattgcc gccgcgatcg ctcacgagca gatttccatc
     5701 ggctcgcatc gcgatgttgc cgaggttgac gaagcccgag gcgaatgttg tgacgctcgt
     5761 gcccgtcgcc ttcttgatga cgttgcccga tgcgaagtag acggtctcgt cgtcggtgac
     5821 ccagaggccg cggccgatcg cgatgccgcc ggtgaccgtc acgagcgtcg tcatcgtgcc
     5881 gtcggtcgcg actttgcgaa tgcggccgtg atcgaggtcg aggatgtaga gcgtgccgtc
     5941 gggcctgacg tagaggccgt tcggattgcc gagcgagcgc tgcgtcgcgg ggcccggcgt
     6001 gtcgtcgacg gcggtggccg tgcccgttcc tgcgactgtg gtgatgacgc cggctggcgt
     6061 gactttgcgg atcgcgtgcg cctctttgtc ggcgatgtag atgttgccgg cggcgtcggc
     6121 cgcagccatg tgcgggcgcg atagctctgc ggacagggcg gggccgcctt cgaatgcggg
     6181 cagccattca tttgcgccgc tgttgtcggt cgcgccggcg cccgcgactg cgagcatcgt
     6241 cccgtagtgt gcgcggacgg aatcccagga gtggtcggca gcatcgatcg tcgacggcgg
     6301 tgcatcgatc gacgcgctgg agcctccaca cgcgacgccg agtgtgactg ctagcgcggc
     6361 gccgattcgt cgcatcccga catgatacgt tgatcgcgag gccgcaggca ccgtggcaga
     6421 gcgtccgctt ccatggatct cgatcgcgat cggcgtcgtc gtgggcgcgg tgctcgcgtt
     6481 cggcaactgt ttgatcggcc tgaagacggg gctgtgggac accgcgcagc tcacgagcac
     6541 gctcctgacg ttcgtgttgt gcgcgccgct gtgccggctc gttggcaaga ccttcgatca
     6601 acacgacaac aacatcgcgc agacgacgag cgcggcgatc gcggccatgc ctgcgaccat
     6661 gggccttctc ggtgcggtgc ccgcgctcgg catgatgggc agcgagccgt cgacggtggt
     6721 gctcgtcgtg tctgcgatct gtttcggcat gctcggcgtc gccagtgcgc tgctcctcgc
     6781 gccgcgcctc atcgaccgcg atcagctgcc gtttccgacc ggcgttgcga cggcggagtt
     6841 catcggcgcg ctgcacacgg atcggcgtgc ggttcgcgag cgcgccctga tgttcggcat
     6901 cgcctttgcg gtgacggcgt tgtttacgct tgtccgcgac gtcggcgagt gggtgcccgc
     6961 gagcatcgag acgacgatcg tcgtcgcggc gatccccgtt gtcgtcggtg tctcggcgag
     7021 cccacttctc gtcggcagcg gtgcgctgat cggcgcgcgc atcgcg
//
LOCUS       DATLBZ010000326         8973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7635704, whole genome shotgun sequence.
ACCESSION   DATLBZ010000326 DATLBZ010000000
VERSION     DATLBZ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7635704"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1014)
                     /locus_tag="VMZ53_17080"
     CDS             complement(61..1014)
                     /locus_tag="VMZ53_17080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HUS30226.1"
                     /translation="MPVVTREQLEASLASLAKDVRNPAEGILGPQSVAWKLGGDLAVF
                     LGGGRAALLQLAHPMVAYAVDQHSKTRSDVVGRFQRTFRNVFAMVFGDLDDAFTAARR
                     VHSVHTRIHGEIPHAVGAYRAHTPYHANDPDALRWVYATLVDTTIAVRERLDGALPSA
                     LKDAYIIEMNRFAALFGIPRDLLAHDWTAHEMYMRRMLSSDEIAVAPVAREMAMFLVG
                     RGGIAPQPPLGRIAEALTSSLLPTRLVHGFGLRTSSLSAAALRIALSTTAPFYRRLPS
                     SAVAIPARSLAASRLVGQPPSRLVAWTERQLFGLTRQVTGS"
     gene            1149..2516
                     /locus_tag="VMZ53_17085"
     CDS             1149..2516
                     /locus_tag="VMZ53_17085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30227.1"
                     /translation="MPTAGSQPAAMPPAVVSEQDDLEPGDVAGPWIVERELGRGGMGS
                     VYAVTHDGIGKRAALKVMHRRLVTSSSAERILQEARVVNQVGHPNIVDIFETGTLPDG
                     RPYIVMERLEGMPLSYRADEGKILPDQVISILLQVCDALIAAHAANIVHRDLKLDNVF
                     LIDNPDDPSQPKVKLLDWGIAKIISADVRHTIEGQLVGTPQYLAPEQARGAAVSAQTD
                     VYSLGVMAYELFLEQLPFEAETSAEIMAMHLRAVPPPPSEMWPDIPPGLENLLLAMLA
                     KDPDSRPTMLTVAHTLEMVRSDLIARREAARESGVSEPALPVRRSTRLKSAPGLAPTE
                     PAASGEWREPSRRWHVAVGAFAIAAASIMFLISRDTETAAASPKQATADLTAPAPVTD
                     NVKPDIAIPQPETAPAATVTHESSPETAKAQTTTRHRSIARKLPVRPRRDASHDPDGT
                     LDPYQ"
     gene            2513..3340
                     /locus_tag="VMZ53_17090"
     CDS             2513..3340
                     /locus_tag="VMZ53_17090"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30228.1"
                     /translation="MTRSALILVALLGCATASGSAQADNALRPEKIPAKAKDLATRGR
                     IYHDAGDYQAAVAAWKEAYVLAPSPGLLFNIAQAYRLAGNCDEAAWMYRRFLDTDPTG
                     PNKALAEQHLASVEKCGRGGLPMMVVTPKIIAKVPDPKNVVKADGSSSTSITTDLPLD
                     EQDKRARTYKRVGIGLGVGAGAALVGAAVFALDANDASNTVEDTYKHGGKWSDVKEAD
                     ARGKRSATLATALGIGGGVALASGVVLYSLGHRYEKAQHVAVVPTGHGAQVSLSWGF"
     gene            3421..3882
                     /locus_tag="VMZ53_17095"
     CDS             3421..3882
                     /locus_tag="VMZ53_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30229.1"
                     /translation="MSETDCAAGQVCGSDRFCASPELAGRCAAMPTGDAGVSVRDAAV
                     HPVDATTITADAKPDGPPPPPTTTLELHVEGQGRISVPGVGICDSAQPDNGDCSFVVL
                     KNVQLQAQAIPHNDWRFDKWTTSPCNIEDSAQCGFTPIAPVNVTAKFRKDD"
     gene            3879..4316
                     /locus_tag="VMZ53_17100"
     CDS             3879..4316
                     /locus_tag="VMZ53_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30230.1"
                     /translation="MKAFAVAALLLASCYSPEALDCTVSCSASDACGDGQVCGSDGFC
                     ADPAVAGHCGGPDGGMPSSLVSLRVMIDGPGKVTVETVGLCDEGGRDGCMFSVQNGRS
                     LQLKAQAKSDKEFVGWMGACSGSSTTCTLTPVAALTQVGARFE"
     gene            4461..5648
                     /locus_tag="VMZ53_17105"
     CDS             4461..5648
                     /locus_tag="VMZ53_17105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30231.1"
                     /translation="MVAHAIPASAATQLACDAPAKNLRRKGRPDDALIPGSRVSSWRV
                     ETELGRGGMASVHAVTHMKFGKRAAIKIAHKSILGDSFSPQTFHREARIVHTVDHPGV
                     IDVFATGTCDGRPYLVMEKLAGQTLGARVDAGPPMDRREAINILLELVDILRATHAAG
                     VVHRDLKLDNVFITDTTYADNRRVKLLDWGVAHVEGEEDPFRGLIAGTLTYVAPEQIR
                     GDALTGAADIYSLAVLAYHLLCRRAPFHAGNDLALIHLHLRAEPPKPSVAWPECPQEV
                     EALLLQMLAKHPEERPSLDVVETVLRDALDIIDVPPRFAEGSSAHIALPVEDPQPWFA
                     RWLTAKPATQQASTTSAVLADPFGRPVLPFAQPFKLGWACVAAGIAVGAGLLSMLSLA
                     AAL"
     gene            complement(5649..6296)
                     /locus_tag="VMZ53_17110"
     CDS             complement(5649..6296)
                     /locus_tag="VMZ53_17110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HUS30232.1"
                     /translation="MADNDSREGKRYTTPAILDYVNKTHAGHDSALAQAFAVPEGIPA
                     IQVGPSEGRLLYLLMKLAGAKKVVEVGTLVGYSAIHMARALPAGGHLWSVEFDPKHAQ
                     VARHNIATAGLADQVTVLVGAGVEMLPTLVDKGPFDAVFIDADKQNYDKYGTWAVDNL
                     RSGGLVLGDNAYLFGELLEDSDRGKAMRGFHEHVAKSCDSVCLPTPDGLVIGIKR"
     gene            complement(6306..7481)
                     /locus_tag="VMZ53_17115"
     CDS             complement(6306..7481)
                     /locus_tag="VMZ53_17115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS30233.1"
                     /translation="MASSTISVRAVPSISQVDAAAWDGLSHGPSPFLRHGFLRALEES
                     GSTGGTGRKRSGWQPVYLLAEIEDRLVGGVAAFVKDHSYGEYIFDWGWANAAQRAGLR
                     YYPKLVIAAPATPATGNRILLAPDAPPIVAEALIKTVRVVADEADCSSIHWLFCTREE
                     QQLLVQHGHFARDTLQFHWKNRGYAKFDDFLGQLKSRKRKQLRKERQKAQSQVERITW
                     LEGRELDQARLDDLDRFYRTTTENHHGRDYLREGFFHRVAETLPDAMRMVEVVQGGQR
                     IAGALFFETPHALYGRYWGCDRHVDLLHFETAYYAGIDRAIEKKLPLFEAGAQGEHKL
                     LRGFEPSATYSAHWIRHEGLSAAIEDYCQRESQAQAVEIAEMAKYGPYRSDNADEDD"
     gene            7493..8419
                     /gene="grxD"
                     /locus_tag="VMZ53_17120"
     CDS             7493..8419
                     /gene="grxD"
                     /locus_tag="VMZ53_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HUS30234.1"
                     /translation="MTLDPQIKSKIDGLVAQDPVVLFMKGTRSFPQCGFSASVVNILN
                     TLIPKYATVNVLSDPDVRGGMKEYSDWPTFPQLFIKGEFVGGADIVRQMFESGDLEKK
                     LGDLVKPAAVPTVTVSPRAAKELGEALKEGSPGDVIHLTITSGWEHQLDIGGKEAAHV
                     TVQTEGVTVQLDRASAARANGVTIDFIEDATGAGFKIENPNRPTMVREIPPKELKALL
                     DSGKVKHFYDVRTPKEREMAVLPNTKLLDDATMKEIEALPKDTPIAFHCHHGSRSRGA
                     ADHFLKQGFKNVYNVAGGIDAWSAIDPSVKRY"
     gene            8421..>8973
                     /locus_tag="VMZ53_17125"
     CDS             8421..>8973
                     /locus_tag="VMZ53_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30235.1"
                     /translation="MRTGWVALVALAACGDDPKPIEPTANLAREIVDTKLSFDVTAKT
                     GTAAITFAPSTEPGASLEVGDLSIDPLAIGGEYLASAQTGAQLDLALPASTEAITVDV
                     SFRYNFHEGFEGASSKGFTLFWPYYCGNLFPCHSQPADGTTFSLDLAGIADGKTAVFP
                     PSIESEAPSYQIAWSIGDYTQLDL"
BASE COUNT         1556 a         3034 c         2892 g         1491 t
ORIGIN      
        1 gggagcctcc cgctttcggc aaaagcactt tcgtgtcggc gcgcgacgca acttcgagag
       61 ctacgagccg gtgacctggc gcgtgagccc gaacagctga cgctccgtcc acgcaacgag
      121 ccggctcggc ggctgaccca caaggcggct ggccgcgagc gagcgagccg ggatcgcaac
      181 ggcgctgctc gggagccggc gatagaacgg cgcggtggtc gagagcgcga tccgaagcgc
      241 ggcggccgag agcgatgacg tgcggagacc aaagccgtgc acgagtcggg tcggcagaag
      301 tgatgacgtc aacgcttcgg cgatgcggcc gagcggaggt tgcggcgcga tgcccccgcg
      361 gccgacgaga aacattgcca tctcgcgcgc gaccggcgcg accgcgatct cgtcgctgct
      421 cagcatgcgg cgcatgtaca tctcgtgcgc agtccaatcg tgcgccagca gatcccgcgg
      481 gatcccgaac agcgcggcga agcggttcat ctcgatgatg taggcgtcct tgagcgcgga
      541 cgggagcgca ccatcgaggc gttcgcgcac ggcgatcgtc gtgtcgacga gtgttgcgta
      601 cacccacctg agcgcatcgg gatcgttcgc gtgatagggc gtgtgcgctc gatatgctcc
      661 cacagcgtgc ggaatctccc cgtggattcg cgtgtgaaca gagtgcacgc gacgcgctgc
      721 agtgaacgca tcgtcgagat cgccgaacac catcgcgaac acgttgcgga acgtgcgctg
      781 aaatcgaccg acgacatcac tgcgtgtctt cgagtgttga tcgacggcgt aagccaccat
      841 gggatgagct agttggagca gcgcagcgcg tcctccgccg aggaagaccg cgagatcgcc
      901 gccgagcttc catgcgactg attgcggccc gaggatgcct tcagcgggat tgcggacgtc
      961 ctttgcgagg ctcgcgagcg acgcttctaa ctgttcacgc gtgacgaccg gcacctccac
     1021 catcctagcg tgcccgtgcg ccacagccgc gccggactgc cacgtaggtg tgcgccaact
     1081 gcccaatggc gtcacttacg gcgtcggcat acgcactgca cagaagacaa gccggaatgc
     1141 agaccttcgt gcccaccgca gggagccagc cggcggcaat gccgcccgcg gttgtcagcg
     1201 agcaggatga tctggagccg ggcgatgtcg ccggcccctg gattgtcgag cgtgagcttg
     1261 ggcgtggtgg catgggttcg gtgtacgcgg tcacgcacga cggcatcggc aagcgtgcag
     1321 cactcaaggt gatgcaccgc cggctcgtta cgagcagcag cgctgaacgc atcctgcagg
     1381 aagcgcgcgt cgtcaaccag gtcggtcatc ccaacatcgt cgacatcttc gagaccggaa
     1441 cgctgcccga tggccgtccg tacatcgtga tggagcgcct cgagggcatg ccgctctcgt
     1501 atcgcgccga cgaaggcaag atcctcccgg atcaagtcat cagcatcctg cttcaagttt
     1561 gcgatgcgct catcgcagcg cacgcggcga acatcgtcca tcgcgatctg aagctcgaca
     1621 acgtgttctt gatcgacaac cccgatgatc cgtcgcagcc caaagtgaag ctgctcgatt
     1681 ggggcatcgc gaagatcatc tcggccgacg ttcgccacac gatcgaaggc cagctcgtcg
     1741 gcacaccgca gtacctcgca cccgagcagg cgcgcggcgc ggcggtgtct gcgcagaccg
     1801 acgtctactc gctcggcgtc atggcgtacg agctgttcct cgagcagctc ccgttcgaag
     1861 cggagaccag cgccgagatc atggcgatgc atcttcgcgc cgtgccacca ccgccaagcg
     1921 agatgtggcc ggacattccg cctggcctcg agaatctcct cctcgcgatg ctcgcgaagg
     1981 atccggactc tcggccgacg atgctgacgg tcgcgcacac gctcgagatg gtccgcagcg
     2041 acctgatcgc tcgtcgcgag gccgcgcgtg agtccggcgt ttccgagccg gctcttccgg
     2101 ttcgtcgttc gacgcgcctc aagtcggcac ccggcctcgc tcccaccgag cccgccgcga
     2161 gtggcgaatg gcgcgagccg agccgccgct ggcacgtcgc cgtcggcgcg ttcgcgatcg
     2221 ccgcggcgtc catcatgttc ctcatcagcc gcgacaccga gaccgctgcg gcttcgccga
     2281 aacaggcgac agcggatctc accgcgccgg ctcccgtgac ggacaacgtc aagccggaca
     2341 tcgcgatccc acaacccgag acggctccgg ccgccacggt tacgcacgag tcctcgcccg
     2401 agacggcgaa ggcccagacg acgacacgtc atcgttcgat tgctcgcaag ctcccggtca
     2461 ggccgcgccg ggatgcatcg cacgaccctg acggcaccct cgatccgtac cagtgacgcg
     2521 ctccgccctc atcctcgttg cgctgctcgg ctgcgcgacg gcgagtggat ccgcgcaggc
     2581 ggacaatgcg ctgcgtccgg aaaagattcc ggccaaggcc aaagatctcg cgacgcgcgg
     2641 ccgcatctat cacgatgccg gcgactacca agcggctgtc gccgcgtgga aagaagccta
     2701 cgtgctcgcg ccgagcccgg gcctgctctt caacatcgcg caggcgtatc gcctcgcggg
     2761 taactgcgat gaagcagcgt ggatgtaccg ccgcttcctc gataccgatc cgacggggcc
     2821 gaacaaggcg ctcgccgagc agcacctcgc gtcggtcgag aaatgcggcc gcggcggtct
     2881 gccgatgatg gtcgtcacgc cgaagatcat cgcgaaggtt cccgatccga agaacgtcgt
     2941 gaaagcggac ggaagctcga gcacgagcat cacgacggat ctaccgctcg acgaacagga
     3001 caagcgcgct cgcacgtaca agcgcgtcgg catcggcctt ggtgtcggcg ccggtgcagc
     3061 gctcgtcggt gcagccgtgt tcgcgctcga cgcaaacgac gcgtccaaca ccgtcgaaga
     3121 cacgtacaag cacggcggca agtggtccga cgtcaaagag gcggacgccc gcggtaagcg
     3181 gagcgcgacg ctcgcgacgg cgctcggcat cggcggcggt gtcgctctcg cttccggcgt
     3241 cgtgctctac agccttggtc atcgctacga gaaggcacaa cacgttgcgg tcgttccgac
     3301 cggtcacggc gcgcaggtca gtctgtcatg gggcttttaa ctcgcgcctt cgcgcttccg
     3361 ttctgcgtgc tgacgtctgc ctgttatgca cctgacctcc gcgactgcac ggtgagctgt
     3421 ttgtccgaga ccgattgcgc cgcgggtcaa gtctgcggca gcgatcggtt ttgcgcgtcg
     3481 cccgagcttg ccggccgctg cgcagcgatg ccgaccggcg atgcgggcgt ttccgtgcgc
     3541 gacgccgcgg tgcatcccgt cgatgcgacc accatcacgg ccgacgcgaa gcccgatggg
     3601 ccgccgccgc caccgacgac gacgctcgag ctccacgtcg aaggccaagg ccgcatcagc
     3661 gtgcccggcg tcggcatctg tgattcggct cagccggaca acggcgactg ttcattcgtc
     3721 gtgctgaaga acgtgcagct gcaggcgcag gcgattccac acaacgactg gcgattcgac
     3781 aaatggacga cgtcgccgtg caacatcgaa gactcggcgc agtgcggctt cacgccgatc
     3841 gcaccggtca acgtcaccgc gaaattcagg aaagacgatt gaaggccttc gctgtcgccg
     3901 cgctgttgct tgcgagctgc tactcccccg aggcgctcga ttgcaccgtc tcgtgctccg
     3961 cgtcggatgc atgcggtgat ggccaggttt gcggcagcga cgggttctgc gcagatccag
     4021 ctgttgccgg tcattgcggc ggtcccgatg gcggaatgcc ttcttcgctc gtgtcgctgc
     4081 gcgtgatgat cgatggtccc ggcaaagtca cggtcgagac cgtcgggctg tgcgacgagg
     4141 gcggccgcga cggctgcatg ttctcggtgc agaacggccg ctcgctccag ctcaaggcac
     4201 aggcgaagag cgacaaagaa ttcgtcggct ggatgggcgc ctgcagcggt tcctcgacga
     4261 cgtgcacgct gacgcctgtc gctgctctaa cgcaggttgg agcgcgattc gaatgaacac
     4321 ctcacgtttg ctcccggcgc acatacgtga gcggcacggt cgtgcggaga agcgcgctcg
     4381 gttacccgag accacagcat tctccgatgc atggggcgca ctctgtgctg gtccgctgca
     4441 tgcacgagca gctgcccact atggttgctc acgcaattcc cgcctccgcg gcaacgcagc
     4501 tggcgtgtga tgcacccgcc aagaatcttc gccgcaaagg acgcccggac gacgcgttga
     4561 tccccggctc gcgcgtcagc tcgtggcgcg tcgagaccga gctcggacgt ggtggcatgg
     4621 ccagcgtcca cgcggtcacg cacatgaagt tcgggaagcg cgcggcaatc aagatcgcgc
     4681 acaagtcgat cctcggcgac agcttctcgc cgcagacgtt ccatcgcgaa gcgcgcatcg
     4741 ttcacacggt cgatcatccg ggcgtcatcg atgtgttcgc gaccggcacg tgcgacggcc
     4801 gtccgtatct cgtcatggag aagctcgccg gccaaacgct cggcgctcgc gtcgatgcgg
     4861 gcccgccgat ggatcgccgc gaagcgatca acatcctcct cgagctcgtc gacatcctcc
     4921 gcgccacgca cgccgctggc gtcgtccacc gcgacctcaa gctcgataac gtcttcatca
     4981 ccgacaccac atacgcggac aaccgccgcg tgaagctcct cgattggggc gtcgctcacg
     5041 tcgaaggcga agaggatccg ttccgcggcc tcatcgcagg cacgctgacg tacgtcgcgc
     5101 ccgagcagat ccgcggtgac gccctgaccg gcgcagccga catctactcg ctcgcggtgc
     5161 tcgcctacca cctcctctgc cgccgcgcgc cattccacgc cggcaacgac ctcgcgctca
     5221 ttcacttgca cctgcgcgca gagccgccga agccgagcgt cgcgtggccc gagtgcccgc
     5281 aagaggtcga agcgctgctc ctgcagatgc tcgcgaagca tccggaagag cgcccgtcgc
     5341 tcgacgtcgt cgagaccgtg ctgcgcgacg cgctcgacat catcgatgtc ccgccgcgct
     5401 tcgccgaagg ctcgagcgct cacatcgcgc tgcccgtcga ggatccacag ccgtggttcg
     5461 cgcgctggct cacggcgaag cccgccacgc agcaggcgtc gacgacctct gccgtgctcg
     5521 ccgatccgtt cggccgaccg gtcctgccgt ttgctcagcc gttcaagctc ggctgggcgt
     5581 gcgtcgctgc cggtatcgcg gtcggcgctg gcctgctcag catgctcagc cttgccgccg
     5641 cgctgtagct agcgcttgat gccgatcacg aggccatcgg gcgtcggaag gcagacggag
     5701 tcgcacgact tcgcgacgtg ctcgtggaag ccgcgcatcg ctttgccgcg atcgctgtcc
     5761 tcgaggagct cgccgaacag gtatgcgttg tcgccgagga cgaggccgcc actgcgcaga
     5821 ttgtcgacgg cccacgtgcc gtacttgtcg tagttctgct tgtccgcgtc gatgaagact
     5881 gcgtcgaacg ggcctttgtc gacgagcgtc ggcagcatct cgacgcctgc gccgacgagc
     5941 accgtcacct gatcggcgag gcccgccgtc gcgatgttgt gacgcgcgac ctgtgcgtgc
     6001 ttcggatcga actcgacgga ccacaggtgg ccgccggccg gcagcgcacg cgccatgtgg
     6061 atcgcggagt agccgacgag cgtgccgacc tcgacgacct tcttcgcgcc tgcgagcttc
     6121 atcaatagat agagcagccg gccttcactc ggtccgactt gaatcgccgg aatgccctcg
     6181 ggtaccgcga aggcttgggc gagcgcggag tcgtgacccg cgtgcgtctt gttgacgtaa
     6241 tcgagaatcg ccggggtcgt gtaccgcttg ccctcgcgcg agtcgttgtc ggccatgcgc
     6301 gaaaactagt cgtcctcgtc ggcgttgtca cttcggtacg gcccatactt cgccatctca
     6361 gcaatctcga cggcctgtgc ctgcgattcg cgttggcagt agtcctcgat cgccgccgac
     6421 agaccctcgt ggcgaatcca gtgcgccgag tacgtcgcgc tcggctcgaa gccgcggagc
     6481 agcttgtgct cgccctgtgc gccggcctcg aacagcggca gcttcttctc gatcgcacga
     6541 tcgatgcctg cgtagtacgc ggtctcgaag tgcagcaaat cgacgtggcg gtcgcagccc
     6601 cagtagcggc cgtagagcgc gtgcggcgtc tcgaagaaca gcgcgcccgc gatgcgttgc
     6661 ccgccctgca ccacttcgac catccgcatc gcatcaggca gcgtctcggc gacgcggtga
     6721 aaaaagcctt cgcgcaggta gtcgcgcccg tgatgattct ccgtcgtcgt gcgatagaag
     6781 cgatcgagat catcgaggcg cgcttgatcg agctcgcgcc cttcgagcca cgtgatgcgc
     6841 tcgacttgcg actgcgcctt ctgccgctcc ttgcgcagct gctttcgctt gcggctcttc
     6901 agctggccga ggaaatcgtc gaacttcgcg tagccgcggt tcttccaatg aaactgcaag
     6961 gtgtcgcgtg cgaagtggcc gtgctgcacg agtagctgct gctcttcgcg cgtgcagaac
     7021 agccagtgga tcgacgagca gtccgcctcg tcggcgacca cgcgcacggt cttgatcagg
     7081 gcttcggcca cgatcggcgg agcgtctggg gcgagcagga tccgattgcc ggtggccggt
     7141 gtggccggcg cggcgatgac gagcttgggg tagtagcgaa gcccggcgcg ttgagccgcg
     7201 tttgcccagc cccagtcgaa gatgtactcg ccgtagctgt ggtctttgac aaacgcggcg
     7261 acgccaccga cgagccggtc ctctatctcg gcgagcaggt agaccggctg ccagccgctg
     7321 cgcttgcgcc ccgtgccgcc cgtcgagccc gactcctcga gggcccgcaa aaacccgtgg
     7381 cgtaggaacg ggctgggacc gtgggacagg ccgtcccacg ccgcggcatc gacctgcgag
     7441 atcgacggaa ccgcgcgtac ggagatcgtg ctagaagcca cctacaacac ctatgacgct
     7501 cgacccgcag atcaagtcga agatcgatgg cctcgtcgcg caggaccccg tcgtcctgtt
     7561 catgaaggga acgcgaagct tcccgcagtg cggattctcg gcgtccgtcg tgaacatcct
     7621 caacaccttg atccccaagt acgccacggt caacgtgctg tccgacccgg acgttcgggg
     7681 aggcatgaaa gagtactccg actggccgac gttcccccag ctgttcatca agggagagtt
     7741 cgttggcggc gccgacatcg tccgtcagat gttcgagtcg ggtgacctcg agaagaagct
     7801 cggcgacctc gtgaagcccg ccgccgttcc gacggtgacg gtttcgccgc gcgccgcgaa
     7861 ggagcttggc gaggcgctga aggagggctc gccgggcgac gtgatccacc tgacgatcac
     7921 gtcgggctgg gagcaccagc tcgatatcgg cggcaaggaa gccgctcacg tcacggtgca
     7981 gaccgaaggc gtgacggtgc agctcgaccg cgcgagtgca gcgcgtgcga acggcgtgac
     8041 gatcgatttc atcgaggacg cgaccggcgc gggcttcaag atcgagaatc cgaatcgccc
     8101 gacgatggtg cgcgagattc cgccgaagga gctcaaggct ctactcgaca gcggcaaggt
     8161 gaagcacttc tatgacgtcc gcactccgaa ggagcgcgag atggcggtgc tgccgaacac
     8221 gaagctcctc gacgacgcga cgatgaaaga gatcgaagcg ctgccgaagg acacgccgat
     8281 cgcgttccac tgccatcacg gcagccggag ccgcggcgcg gccgatcact tcctgaagca
     8341 aggcttcaag aacgtctaca acgtcgccgg cggcatcgac gcctggtcgg cgatcgatcc
     8401 ctcggtcaag cgctactagc gtgcgcaccg ggtgggtggc gctcgtcgcg ctcgcggcgt
     8461 gcggtgatga tccgaaaccg atcgagccga cggcgaacct cgcacgcgag atcgtcgaca
     8521 cgaagctgtc gttcgacgtc accgcgaaga ccggcacggc ggccatcacg ttcgctccct
     8581 cgacggagcc gggcgcgtcg ctcgaggtcg gcgacctcag catcgatcca ctcgcgatcg
     8641 gcggcgagta cctcgcatcg gcgcagaccg gcgcgcagct cgacctcgcg ctgcctgcat
     8701 cgaccgaggc gatcaccgtc gacgtgtcgt ttcgctacaa cttccacgaa ggcttcgaag
     8761 gcgcgtcgtc gaagggcttc acgctgttct ggccgtacta ctgcggcaac ctgttcccct
     8821 gccactcgca gcccgcagac ggcacgacgt tctcgctcga tctcgccggc atcgccgacg
     8881 gcaagaccgc cgtgttcccg ccgtcgatcg agagcgaagc gccgtcgtac caaatcgcgt
     8941 ggtcgatcgg cgactacacg cagctcgacc tcg
//
LOCUS       DATLBZ010000327        12045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1118190, whole genome shotgun sequence.
ACCESSION   DATLBZ010000327 DATLBZ010000000
VERSION     DATLBZ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12045
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118190"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1010)
                     /gene="rarD"
                     /locus_tag="VMZ53_17130"
     CDS             complement(114..1010)
                     /gene="rarD"
                     /locus_tag="VMZ53_17130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HUS30236.1"
                     /translation="MTRSSYRVGLLQGVLAYGLWGIVAAYWKLLSHVSPVELIAHRAV
                     WGLGSFLIIVVVAGQWRPFVTALRDLRLVGVMALSSFLLAINWGVFVGATTHGHLLDA
                     SLGYFINPLVSIALGTLVLRERLRRLQWIAIALAAIGVGVLTWRAGRIPWIALVLAGT
                     WGLYGLVRKTARVDALVGSTIETVLLAPIAAVYLIAIGGGVMPTATASTNALLVGTGV
                     ITVVPLVLFTSAARRLPLSTVGFLQYLAPTGQFLLAVLAFGEPLAHDRLMAFAWIWAG
                     LAAFSIDRARQAAAASASQKST"
     gene            1043..1870
                     /locus_tag="VMZ53_17135"
     CDS             1043..1870
                     /locus_tag="VMZ53_17135"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HUS30237.1"
                     /translation="MTARVLHQSESITAIDYRCTARRHERPFAEVHTSYSIAYVRSGS
                     FGCKARGRAFELVRGSLLLGRPGDEYTCTHDHAEGDECLAFMISDALVDELGGWQTGA
                     APPLAQLVVLGELAQAAASNDAELGVDEVGLSLAARFARVVHDKTSSLMPSPRDRKRA
                     VEIAMWIADHAHEPLDLDTLAARAGLSAYHFLRVFSAVLGVTPHQYVVRSRLLHAARL
                     LADAERSITDVAFDVGFADVSNFVRTFHKAAGVSPRQFRRAARGDRKILQDRIAARP"
     gene            1894..2256
                     /locus_tag="VMZ53_17140"
     CDS             1894..2256
                     /locus_tag="VMZ53_17140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS30238.1"
                     /translation="MFDHIGLQVKDLAAAVRFYEAVLKPLGFVLCSRDETSAGFGPKD
                     APALWLYADNKAKAARTHIALKAPTRKAADAFHAAGLESGGSDNGKPGIRADYAPNYY
                     AAFLIDPDGNNIEAVCYH"
     gene            2260..2901
                     /locus_tag="VMZ53_17145"
     CDS             2260..2901
                     /locus_tag="VMZ53_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30239.1"
                     /translation="MLRLAIALMLVVAAPVRADALCKGCIFELPKDAEKPPLVVVLHG
                     DREHAKNAAARWRSTVKAKAFALLALECPKSEGCKDSWWKWDGDPQWVLDQIAAAGKQ
                     HPVARIYLVGWSGGATYLGWHPKAWEGVASGIVFHGGGSAPSDQTCVTLPAYFLVGDK
                     NPLHYLVKTLREYFDTCKQDIVWDLVKGGDHAREAKALTPKKAAAILDWLDAH"
     gene            2941..3117
                     /locus_tag="VMZ53_17150"
     CDS             2941..3117
                     /locus_tag="VMZ53_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30240.1"
                     /translation="MTGLRVAKSETRDLAERDRVQLLRALNQPTDWQMSNSTFFVAFV
                     VIAAVLTILALILR"
     gene            3127..4455
                     /locus_tag="VMZ53_17155"
     CDS             3127..4455
                     /locus_tag="VMZ53_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30241.1"
                     /translation="MIIDVTTSGELLAAIQSAQPGDTIVLADGTYALTGANCTTTATE
                     TLPITVRGATPLGAHIEFDGLEGFKVSGANWHFENLDVRGVCANDTNCEHAFHVSGAA
                     DGFVLRGSRVHDFNAQLKVNASMIGGNWVMPNRGLVEYNELFDTHGRNTSNPVTKLNI
                     DTGNDWIIRGNFIHDAYKMGGDGVSYATFMKSGGKRGLIERNLVVCSRDTAGGTRIGL
                     SFGGGGTAPQYCAPAYDASTPCSVEHEGGTMRNNIIASCNDVGIYINRGKDSHILYNT
                     LVATNGIDFRFDTTSGEAVGNVMTSVPRTRDGGTMTAGANMENVAVVKFQEWYRAPLT
                     GDLAAIGDVTSLVGGGIARTDVPNDYCGATRPAGKISLGAIEHAHLGCETVPPPHADD
                     PIDPGMGDGSGGDDESPTDGGCCDSSGGAGSLPLGLGVLIALAGIGRRRR"
     gene            complement(4396..5121)
                     /locus_tag="VMZ53_17160"
     CDS             complement(4396..5121)
                     /locus_tag="VMZ53_17160"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HUS30242.1"
                     /translation="MRPNMERVGFTSTLGIDIWSDIVCPWCYIGKRRFEAALAKFSHA
                     ADVEVTFHSFELDPRSVRVHEGNHVEYLSKKFRVSAAQAQQMIDRVTANAAEAGLEFH
                     LDRARGGNTFDAHRLLHLAKARGVQAPLKERLMRAYFTEGQSIAEHDVLAKLAGEVDI
                     AEADARETLASDRFTDDVRGDEQMAGELGIQGVPFYVLGGRLGLSGAQPPDVMLQALE
                     RAWQLSSTTADAGESDQHAEPER"
     gene            5216..5653
                     /locus_tag="VMZ53_17165"
     CDS             5216..5653
                     /locus_tag="VMZ53_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30243.1"
                     /translation="MDKTQVYSGVLRIEDENVVIEGAQKSVLAEPGIGVWIMLYGCSV
                     TAHTRRGFIERVEIDQADCMYREVGPVELVRGTLVVETGAPGSKLAGSRTNYFDSDGD
                     RYGVIGGHIPDEGREVTVRVRKLLANMAYAARATERDIWFEED"
     gene            complement(5654..7126)
                     /locus_tag="VMZ53_17170"
     CDS             complement(5654..7126)
                     /locus_tag="VMZ53_17170"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HUS30244.1"
                     /translation="MTRSAVWTFAFLAATSTTALAGTWHVSTTGSDTAAGSQAAPFKT
                     IQRAVTAANAGDTVLVHAGTYLGFQVTKSGTASAPITFKGEAGVNINGAGTTDRDAIH
                     IENASYVVVDGFTVTGAKRAGLSAITSDHITVRNSRMDQNVNWGVFTAFTDELLVEDN
                     ELSRSTNQHGVYASNSADHPVIRRNYIWGNGMCGVHMNGDISMGGTGIITNAVVENNV
                     IENNGALGGSGINGDGVKNAIIRNNLLDQNHASGISLYMIDGGAPSTGNLVINNTIRQ
                     ASNGRSVINIQDGSTGNTIRNNILLHTSTSKAAIDICATCITGTVSNNNAVVGRFLKG
                     GTMIDLATWRAQTGNDMASFVATDADLFVGASDLTLKTGSMAIDKGVATGAPGSDIDG
                     TMRPQGAAVDIGAYEHCEGTCAGTTGGGGDGSGDGSGDGSGDGSGDGSGSDGTGPDYT
                     GPTADPADGTGGCSAGGGAPGLLLVVIAGLVMRRKARDVY"
     gene            complement(7203..8468)
                     /locus_tag="VMZ53_17175"
     CDS             complement(7203..8468)
                     /locus_tag="VMZ53_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30245.1"
                     /translation="MRYSFGDAAGYGAMVGAAETYLPAFGLALGMSAVSAGLLASVPL
                     LVGGVVQLVAPRAIARVSSMRTWVACATAIQALAFVPLIILALTRTSSAPLVFAAVSL
                     YWAAGNCAAAGWMPWMARVVPARVRARFIGRRAGLVQAMTLMGLIGAGVALHTHANES
                     GHGPMWIYAGMFALALLSRLVSTYCVLRMGRGVDPTPRRRTRLRSLPPKLWGTPRGSL
                     LGYLVAMGAAAAVSGPFLTPYLLLQEHLDYAQYCAFTATIFVVKIVMLPVVGKLIPRV
                     GLRRVLTLSAIAIVPIPFMWLIGGHFWWYIVIQAYAGIAWAGFDLGMLMALFDAEDDS
                     ERITLQVMFSALQAIGNAGASLVGGFLLGTFGADHRAYLIVFMVSAASRIAATTMLVR
                     ELPRLIVRFPQLVLSRAWILLARRDRPSP"
     gene            complement(8614..9327)
                     /locus_tag="VMZ53_17180"
     CDS             complement(8614..9327)
                     /locus_tag="VMZ53_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30246.1"
                     /translation="MLTILIVIGVTTVCVAALRPRRDRNALPPAGGPPEDRGEAVRRV
                     LKHLQRKAIVDVQEGTAAVIVGTVHAVPGIEPIVSPITNESCLGYHVDIRSAVFDEHM
                     RLRQLKDEAACTAFEVRDETGAIRIDPAGLELAITDTAAVLRYPQHPPEIRQRIPGWA
                     FHLPITVEVGVLQPGMRVLVCGVAARELTPTDYRDGTPALLLRASATFPLVASTDADL
                     FHPGDRPISPEELHRARDR"
     gene            9358..10074
                     /locus_tag="VMZ53_17185"
     CDS             9358..10074
                     /locus_tag="VMZ53_17185"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUS30247.1"
                     /translation="MLRNAVVIGLIASLTSVASADDEWTGAQPAPPPSREPAPPDDPQ
                     PPPPPPPTSPPPATTPDLLHTPAPPPAVMNRRWSVGLSFGWETLTIQHGDKQKVTFGM
                     LELAGRFRITPAIEVALALQGGGTEGDIGLGGIYADFRYRFRVERPWNLYAFAGLGAV
                     NVAQKMASDDEKAGRGSLRLGGGLERRFTAFALNVEVRLASVGENKKAPEPVMPSTAN
                     QLSRYGLGGGAVMIGVTYYF"
     gene            10100..10777
                     /locus_tag="VMZ53_17190"
     CDS             10100..10777
                     /locus_tag="VMZ53_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30248.1"
                     /translation="MKRSPRAALLLVAGGVGLFVILGVLVRAVHSEAPESSPEITSRS
                     GNGSTTTQSPASGTGPFASRDSSGRKQSIWRAPGFRVGRSDDSSTASAAVADESSAAK
                     TGDTQNLHFGGTQLRAQTKAISPWVQRCVDDAVKKGQSPSGTAMLTYVVAKHGDKYEV
                     EDSGIDDEQTTIQNAELLDCMRTAAFAMKFEGLPRDATGIVASRKVTLEAGRLVEHKH
                     VTFSYLH"
     gene            10898..>12045
                     /locus_tag="VMZ53_17195"
     CDS             10898..>12045
                     /locus_tag="VMZ53_17195"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HUS30249.1"
                     /translation="MKKNLLAAILLSAACGSNPPPTTTTPSTGTGTETASSTTSEQTS
                     GAQPASVSMSTKGKPEYGTFGFDTNGMDKKTAPGQSFYNFANGGWVKATPIPADKSNY
                     GMFTVLSDKSDERTKEIILNAKGAQGTDAQRIADFYGSFMDEAAIEKAGIEPIKGDLA
                     KIDAIKDKNALVLAFAANSRQMDNTPFETGVAQDDKDPEHYIAVLGQGGLGLPDRDMY
                     DAKNKQFEPMRQGYKKYLENMLTLAGAKDAAKKAAAVYALEEKIAKTHWTRIQNRDPQ
                     KTYNKMTMAELQKLAPSVDWKSWSKVVGYEGQTAINVNQPTAITGIAKLVKSESLGVW
                     KDYMTVHLVSDAAPFLSKKFVDTRFEFYGKTLSGTPELKARWKRGVDYV"
BASE COUNT         2247 a         3890 c         3989 g         1919 t
ORIGIN      
        1 gctcgagcga ggcgcggcga cgctcgacga gcccgatgtg aagaagctcc tcgacgctgc
       61 gttcgatgtc gcggtcatcg cgccggccgg gaaggcgccg ccggtcaccg tgatcaagtc
      121 gatttctgcg aagcagaggc cgcggcggcc tggcgagccc gatcgatcga gaacgcggcg
      181 agccccgccc aaatccaagc aaacgccatc agccggtcgt gcgcgagcgg ctcgccgaac
      241 gcgagcaccg cgagcaagaa ctgcccagta ggcgcgaggt actggagaaa gccgacggtc
      301 gagagcggca ggcgccgcgc ggcgctcgtg aacaaaacga gaggcacgac ggtgatcacg
      361 cctgttccga cgagcagcgc gttcgtcgac gcggtcgccg taggcatgac acctccgccg
      421 atcgcgatga gatagacggc agcgatcggc gcgagcagca ccgtctcgat cgtcgagccg
      481 acgagcgcat cgacgcgcgc tgtcttgcgg acgagaccgt agaggcccca cgtgcccgcg
      541 agcacgagtg cgatccacgg gatgcgaccc gcgcgccagg tcagcacgcc gactccgatg
      601 gccgcgagcg cgatcgcgat ccactgcaag cggcgcagcc gctcgcgcaa cacgagcgtg
      661 ccgagcgcga tcgagacgag cggattgatg aagtagccaa gcgatgcgtc gagcaggtga
      721 ccgtgcgtcg tcgcgccgac gaacacgccc cagttgatcg cgagcagaaa gctcgacagc
      781 gccatcacgc cgacgagcct gagatcgcga agcgcggtga cgaacggccg ccactgccct
      841 gcgacgacga cgatgatgag gaacgagccg aggccccaca ccgcgcggtg cgcgatcagc
      901 tcgacgggac tgacgtgcga gagcagcttc cagtacgccg cgacgatgcc ccacagccca
      961 tacgcgagca cgccttgcag caggcctacc cggtaggacg atcgcgtcat ctgacggatg
     1021 cgtagcacgt taagatagag cggtgactgc cagggttctg caccagagcg agtcgatcac
     1081 cgcgatcgat tatcggtgca ctgcgcgtcg tcacgaacga ccgttcgcgg aggtccacac
     1141 gagctactcg atcgcgtacg tgcgcagtgg cagcttcggc tgcaaggcgc ggggccgcgc
     1201 attcgagctc gtgcgcggat cgctgttgct cggacgcccc ggcgacgaat atacgtgcac
     1261 gcacgatcat gccgaaggcg acgagtgcct tgcgttcatg atcagcgacg cgctcgtcga
     1321 cgagctcggc ggttggcaga ccggcgcggc gccgccgctc gcgcagctgg tcgtgctcgg
     1381 cgagctcgct caggccgcgg cctcgaacga tgcggagctc ggcgttgacg aagtggggct
     1441 gtcactcgca gcccggttcg cgcgcgtcgt tcacgacaag acgtcgtcgc tgatgccgag
     1501 cccacgcgat cgcaaacgcg cagtcgagat cgcgatgtgg atcgcagatc acgcgcacga
     1561 gccactggac ctcgacacgc tcgcggctcg cgcgggcctg agcgcgtacc actttcttcg
     1621 cgtgttcagc gcagtgctgg gcgtgacgcc gcaccagtac gtcgtgcggt cgcggctcct
     1681 ccacgccgcg cgtctcctcg cggacgcgga acgctcgatc accgatgtgg cgttcgacgt
     1741 cgggttcgca gatgtctcga acttcgtgcg gacgtttcac aaggccgcgg gtgtgtcgcc
     1801 gaggcagttt cggcgtgctg cacgaggtga ccgcaagatt ctccaagatc gaatcgccgc
     1861 gcgtccgtag cgtgggcctc acaggaggca aacgtgttcg atcacatcgg tctgcaagtg
     1921 aaggatctcg ccgccgccgt gcggttctac gaagcggtgc tgaagccgct cggtttcgtg
     1981 ctgtgctcgc gcgacgaaac gtccgcgggc tttggcccca aagacgcgcc ggcgctgtgg
     2041 ctctatgccg acaataaagc gaaggcagcg cgcacgcaca tcgcgttgaa agccccgacg
     2101 cgcaaggctg ccgacgcgtt ccatgcggcc gggctcgaga gcggcggcag cgacaacggc
     2161 aagcccggca ttcgcgccga ctacgcaccg aactattacg cggcgttcct catcgatccc
     2221 gacgggaaca acatcgaggc ggtctgctat cactgaggag tgctgcggct cgcgatcgcg
     2281 ttgatgctcg ttgtggcagc accggtccgt gcggatgcac tctgcaaggg ttgcatcttc
     2341 gagctgccga aggatgcgga gaagccgcca ctcgtcgtcg tgctgcacgg cgatcgcgag
     2401 cacgcgaaga acgctgcggc gagatggcgg tcgaccgtga aggcgaaagc ctttgcgctg
     2461 ctcgccctcg agtgtccgaa gtcggaaggc tgcaaggaca gctggtggaa gtgggacggc
     2521 gatccgcagt gggtgctcga tcagatcgcg gccgcgggca agcagcatcc agtcgcgcgc
     2581 atttatctcg tcggttggtc cggcggcgcg acgtaccttg gttggcaccc gaaagcgtgg
     2641 gagggcgtcg cgagcggcat cgtgttccac ggcggcggct cggctccgtc cgatcaaaca
     2701 tgcgtaacgc tgcctgcgta ttttctcgtc ggtgacaaga acccgctgca ttacctcgta
     2761 aagacgctgc gcgagtactt cgatacctgc aagcaagaca tcgtgtggga cctcgtgaag
     2821 ggcggcgacc atgcgcgcga ggcaaaggcg cttacgccga agaaggccgc cgcaattctc
     2881 gactggctcg acgcccacta ggaattggca ttcatcccta gcgcgcggca cactgcgctc
     2941 atgaccggcc ttcgagttgc caagtcggag acgcgcgacc tcgccgagcg cgatcgcgtc
     3001 cagctcttgc gggcattgaa tcagccgacc gattggcaga tgagcaactc gacgttcttc
     3061 gtcgcgttcg tggtcatcgc cgcggttctg acgattctcg cgctcatcct gcgataacct
     3121 catcgcgtga tcatcgacgt cacgacgtcg ggggagcttc ttgctgcgat ccagagcgcg
     3181 cagccggggg atacgatcgt gctcgccgat ggcacgtacg cgctcaccgg cgcgaattgc
     3241 acgacgaccg cgaccgagac gctgccgatc actgtccgcg gagctacgcc gctcggcgct
     3301 cacatcgaat tcgacggcct cgaaggcttc aaggtcagtg gcgcgaactg gcacttcgag
     3361 aatctcgacg tccgcggcgt ctgcgcgaac gacaccaact gcgagcatgc gtttcatgtc
     3421 agcggcgcgg ccgacggctt cgtgctccgc ggttcgcgcg tgcacgactt caacgcgcag
     3481 ctgaaggtca acgcgtcgat gatcggcggc aactgggtca tgccgaatcg ggggctcgtc
     3541 gaatacaacg agctgttcga tacgcacggc cgcaacacga gcaatccggt cacgaagctc
     3601 aacatcgata ccgggaacga ctggatcatc cgcggcaact tcatccacga cgcgtacaag
     3661 atgggtggcg acggcgtcag ctacgcgacg ttcatgaaga gcggaggcaa gcgcggcctc
     3721 atcgaacgca acctcgtcgt gtgctcgcgc gataccgcgg gtggaacgcg catcggtctg
     3781 tcgttcggtg ggggaggcac cgctccgcag tactgcgcgc cggcgtatga cgcgagcacg
     3841 ccgtgcagcg tcgagcacga gggcggcacg atgcggaaca acatcatcgc gagctgcaat
     3901 gatgtcggca tctacatcaa tcgcggtaag gactcgcaca tcctctacaa cacgcttgtc
     3961 gccaccaatg gcatcgactt tcgcttcgac accacgagcg gcgaagccgt cggcaacgtg
     4021 atgacgagcg ttccgcgaac gcgtgatggc gggacgatga ccgcgggcgc gaacatggag
     4081 aacgtcgctg tcgtgaagtt ccaggagtgg tatcgcgcgc cgctgaccgg ggacctcgca
     4141 gcgatcggcg acgtgacgag cctggtcggc ggaggcatcg cgcgcaccga cgtgcccaac
     4201 gattactgcg gggccacgcg tcctgccggc aagatctcgc tcggcgcgat cgagcacgcg
     4261 cacctcggtt gcgagacggt gcctcctccg cacgccgacg atccgataga tcccggaatg
     4321 ggcgatggca gtggcggcga tgacgaatcg ccgacagacg gcggctgctg cgattcgtcg
     4381 ggcggcgcgg gctcgctacc gctcgggctc ggcgtgctga tcgctctcgc cggcatcggc
     4441 cgtcgtcgac gatagctgcc acgctcgctc gagtgcttgc aacatcacgt cgggtggctg
     4501 cgcgcccgag agaccgagtc gacctccgag gacgtagaac ggcacgcctt gaatcccgag
     4561 ctcgccggcc atttgctcgt cgccgcgcac gtcatcggta aagcgatcgc tcgcgagagt
     4621 ctcacgcgcg tcggcctcgg cgatgtcgac ctcgccggca agctttgcga ggacatcgtg
     4681 ctccgcgatc gattgccctt cggtgaagta cgcgcgcatc agccgctcct tcagcggtgc
     4741 ttgcacgccg cgcgccttcg cgaggtgaag caggcgatgc gcatcgaacg tgttgccacc
     4801 gcgcgcgcgg tcgagatgaa actcgagacc agcctcggcg gcattcgccg tgacgcggtc
     4861 gatcatctgc tgcgcttgcg cggcggacac gcgaaacttc ttcgacaggt actcgacgtg
     4921 gttgccctcg tgaacacgca cggagcgagg gtcgagctcg aagctgtgaa atgtgacttc
     4981 gacatcggcg gcgtgcgaaa acttcgccaa cgcagcttcg aaccggcgct tgccgatgta
     5041 acaccagggg cagacgatgt cggaccaaat gtcgatcccg agggtcgagg tgaatccgac
     5101 cctctccatg ttggggcgca cgctcgcacc gtacctcgcg gtgtgcgtcg cggcatgctg
     5161 gacggggtcg gcaccggtgc aacagaccgt tacgaaagca cccgaggttc atcgcgtgga
     5221 caagactcag gtctatagcg gcgtgttgcg catcgaggac gaaaacgtcg tgatcgaagg
     5281 cgctcagaag tccgtcctcg cagagcccgg catcggcgtt tggatcatgc tgtacggctg
     5341 cagcgtgacg gctcacacgc gccgcggctt catcgagcgc gtcgagatcg accaggccga
     5401 ctgcatgtat cgcgaagtcg ggccggtcga gctcgtgcgt gggacgctcg tcgtcgagac
     5461 aggcgcgccg ggctcgaagc tcgcgggcag ccggacgaat tacttcgact ccgacggcga
     5521 tcgctacggc gtgatcggcg ggcatattcc cgacgagggc cgcgaggtca cggtgcgcgt
     5581 gcgcaagctg ctcgcgaaca tggcgtacgc cgctcgcgcg acagagcgcg atatctggtt
     5641 cgaggaagac tagctagtag acgtcgcgcg ctttgcggcg catgacgaga cccgcgatga
     5701 cgacgagcag aaggccgggc gctccaccac cggccgagca accgcccgta ccgtccgcgg
     5761 gatccgccgt gggccccgtg tagtccgggc ccgtgccgtc gctgccgctg ccatcgcccg
     5821 agccgtcgcc cgagccatcg cccgagccgt cacccgagcc gtcgccgccg ccacccgtcg
     5881 ttcccgcgca cgtgccctcg cagtgctcgt acgcgccaat atcgaccgcg gctccctgcg
     5941 gacgcatcgt gccgtcgatg tcggagccag gcgcgccggt cgcaacgccc ttgtcgatcg
     6001 ccatcgagcc cgtcttgagc gtcaggtccg acgcgccgac gaagaggtcg gcatcggtcg
     6061 cgacaaacga cgccatgtca ttgccggtct gcgcgcgcca cgtcgcgagg tcgatcatgg
     6121 tgccgccctt gaggaagcgc ccgacgaccg cgttgttgtt cgacaccgtg ccggtgatgc
     6181 acgtcgcgca gatatcgatc gcggccttgc tcgtcgacgt gtggagcagg atgttgttgc
     6241 gaatcgtgtt gcccgtcgag ccgtcctgga tgttgatgac cgagcggcca ttcgacgcct
     6301 gacgaatcgt gttgttgatg acgaggttgc ccgtcgaagg cgcgccaccg tcgatcatgt
     6361 agagcgagat gcccgacgcg tggttctggt cgagcaggtt gttgcggatg atcgcgttct
     6421 tgacgccgtc accgttgatg ccggagccgc cgagcgcgcc gttgttctcg atgacgttgt
     6481 tctcgacgac cgcattcgtg atgatgccgg tgccgcccat gctgatgtcg ccgttcatgt
     6541 gcacgccgca catgccgttg ccccagatgt aattgcggcg gatgaccggg tggtcggcgc
     6601 tgttcgacgc gtagacgccg tgctggttcg tcgagcgaga cagctcgttg tcctcgacga
     6661 gcagctcgtc cgtgaacgcc gtgaagacac cccagttgac gttctgatcc atccgagagt
     6721 ttcgcaccgt gatgtggtcg ctcgtgatcg ccgagaggcc cgcgcgcttc gctccggtga
     6781 cggtgaagcc gtcgaccacg acgtagctcg cgttctcgat gtgaatcgcg tcgcgatcgg
     6841 tcgtgccggc gccgttgatg ttcacgcccg cctcgccctt gaacgtgatc ggcgcgctgg
     6901 ctgtacccga cttcgtcact tgaaagccga ggtacgtgcc cgcgtgaacc aggaccgtgt
     6961 cacctgcatt ggccgcggtc accgcacgtt ggatcgtctt gaacggcgca gcctgcgagc
     7021 cagcagcggt gtcgctacca gttgtcgaga cgtgccatgt acctgcgagg gccgtcgtcg
     7081 aagttgctgc gagaaatgcg aaggtccaaa cagctgatct cgtcatctgc tgcggacact
     7141 aactagtgcg ttaacgctcg tcgctgtcgt tcttagcgca cagccgcaca tcggtagcgt
     7201 gcttagggtg atggacgatc gcgcctcgcg agcaagatcc acgcacgaga aagtacgagc
     7261 tgaggaaagc gaacgatcaa gcgaggtagc tcgcgcacca gcatcgttgt cgctgcgata
     7321 cgtgaagcag ccgacaccat gaaaacgatc aaataagctc tgtggtccgc accgaatgtg
     7381 ccaagcagga atccaccgac gagcgacgcg ccggcgtttc cgatcgcctg cagcgcgctg
     7441 aacatcactt gcagcgtgat tctctcgctg tcgtcctcgg catcgaacag cgccatcagc
     7501 atgccgagat caaagccggc ccacgcgatg cctgcgtacg cttgaatcac gatgtaccac
     7561 cagaagtggc caccgatgag ccacatgaac ggaatgggca cgatcgcgat cgccgagagc
     7621 gtgagcacgc gacgcaaccc gacgcgcggg atgagcttgc cgacgacggg cagcatcacg
     7681 atcttgacga cgaagatcgt ggcggtaaac gcgcagtact gggcgtaatc gaggtgctcc
     7741 tgcagcaata gatacggcgt gaggaacggt cccgagaccg ccgccgctgc gcccatcgcg
     7801 acgagatatc cgagcaacga gccgcgcggt gttccccaca gcttcggcgg taggctgcga
     7861 aggcgcgtcc ttcgtcgagg agttggatcg acgccgcgtc ccatgcgcag gacgcagtac
     7921 gtcgacacca ggcgtgacag cagcgcgagc gcgaacatgc ccgcgtagat ccacatcggg
     7981 ccatgaccgc tctcgttcgc gtgcgtgtgg agcgcgacgc ccgcgccgat gaggcccatc
     8041 agcgtcatcg cttgcacgag accggcgcgc cgcccgatga aacgcgcacg cacacgcgca
     8101 ggaaccacgc gcgccatcca tggcatccag ccggcagcag cgcagttgcc tgcagcccaa
     8161 tacagcgaga ccgccgcgaa cacgagcggt gccgacgacg tgcgcgtcag tgcgaggatg
     8221 atgagtggta cgaacgcgag tgcttggatg gccgtcgcgc acgcgaccca cgtgcgcatg
     8281 ctcgagaccc gcgcgattgc acgaggcgcg acaagctgca cgacgccacc gacgaggaga
     8341 ggcacgctcg cgagcagacc ggcgctgacc gcggacatcc cgagcgcgag accgaacgcg
     8401 ggcaaatagg tctcggccgc gccgaccatc gcgccgtagc cggcggcatc gccgaacgag
     8461 taccgcatgt cacgacgcac gccgcgcgcg ggtctcatcc agcgtccgga catgctgaaa
     8521 gccatcgcgc gatcgcggct ttcaccaccc ggtggacgca cgccccagag gtagcacgcg
     8581 gtcgtcgatg cggcctgttt ctgcgaggag ctctcagcgg tctcgagcgc gatgcagctc
     8641 ttcaggagag atcggacgat cgcctggatg gaatagatcg gcgtccgtcg atgcgacgag
     8701 cggaaacgtt gcggacgcgc gtaagagaag cgcgggcgtg ccgtcgcgat agtcggtcgg
     8761 tgtgagctcg cgagctgcga cgccgcagac gagcacgcgc atacccggct gcaggacccc
     8821 gacctcgacg gtgatcggta aatgaaatgc ccagcccgga atgcgctggc ggatctcggg
     8881 aggatgttgc ggatagcgca ggacggcggc cgtatccgtg atcgcgagct cgagccctgc
     8941 tggatcgatg cggatggcgc cggtctcatc gcgcacttcg aatgcagtgc acgccgcttc
     9001 gtccttgagc tggcgcagcc gcatgtgctc gtcgaagacc gcagaacgaa tgtcgacgtg
     9061 atagccgagg cagctctcgt tcgtgatcgg tgacacgatc ggctcgatgc cgggaactgc
     9121 gtggacggtg ccgacgatca ccgcggcggt gccctcctgc acgtcgacga tcgctttgcg
     9181 ctgcaggtgc ttgagcacgc gccgcaccgc ttcgccgcga tcttcgggcg gaccacccgc
     9241 aggcggcagc gcgttgcgat ctcgccgagg acgcagcgcc gcgacgcata cggtcgtgac
     9301 accgatgacg atcaagatcg tcaacaccgg cggcgactgt aggcgataat gggggagatg
     9361 ttgcgcaatg ccgttgtcat cggactcatc gcgtcgctca cgtccgtcgc gagcgccgac
     9421 gacgaatgga ccggcgcgca acctgcgcca ccgccgtcga gagagcccgc gccacccgac
     9481 gacccgcagc cgcctccgcc tccgcctccg acgagcccgc cgccggcgac gacgcctgat
     9541 ctgctacaca cgcccgcgcc accgcctgcg gtgatgaatc ggcgctggtc cgtcgggctc
     9601 tcgttcggct gggagacgct cacgatccag cacggcgaca agcagaaggt cacgttcggc
     9661 atgctcgagc tcgccggcag attccggatc actccggcga tcgaggtcgc gctcgcattg
     9721 caaggcggcg gcaccgaggg cgacatcggc ctcggcggca tctacgcaga cttccgctac
     9781 cggtttcgcg tcgagcggcc gtggaacctc tacgcgtttg ccggcctcgg cgccgtgaat
     9841 gtcgcacaga agatggcaag cgatgacgag aaggccgggc gcggatcgct gcgcctcggt
     9901 ggtggtctcg agcgccgctt caccgcattc gcgctgaacg tcgaggtgcg gctcgcgagc
     9961 gtcggcgaga acaagaaagc tcccgagcct gtgatgccga gcaccgcgaa tcagctctca
    10021 cgctacgggc tcggcggcgg tgcggtgatg atcggcgtca cctactactt ctagagcgag
    10081 atgcgatagt gtgcccgtga tgaaacggtc tcctcgcgcc gcgctgctgc tcgtcgccgg
    10141 cggggtaggc ctcttcgtga tcctcggtgt gctcgtgcgc gccgtgcaca gcgaagcacc
    10201 ggaatcgtcg ccggagatca cgagccgcag cggcaacgga tcgacgacca cgcaatcgcc
    10261 cgcatcgggc accggtccgt tcgcgtcgcg cgattcttcg ggacgcaaac aatcgatctg
    10321 gcgtgcgccc ggttttcgcg tcggacgcag cgacgactcg agcacggcca gcgctgcagt
    10381 cgccgacgag agctccgccg cgaagacagg cgacacgcag aacctgcatt tcggcggcac
    10441 gcaactgcgc gcgcagacga aggcgatcag cccgtgggtg cagcgctgtg tcgacgatgc
    10501 cgtcaagaag ggtcagtccc cgagcgggac cgcgatgctg acgtatgtcg tcgcaaagca
    10561 cggcgacaag tacgaggtcg aggacagcgg catcgatgac gagcagacga cgatccagaa
    10621 tgccgagctc ctcgactgca tgcgcacggc cgcgttcgcc atgaaattcg agggtctgcc
    10681 gcgtgatgca actggcatcg tggcgtcgcg caaggtcacg ctcgaggccg gccggctcgt
    10741 cgagcacaag cacgtgacct tctcgtacct tcactagagt gcgagccgcg cgcctcagcg
    10801 tctaaccgat atctggcgtg tccttgcctg cggcaggggt gcggcatcct tacgtcgcat
    10861 ctaggcaagc tcgtcgagag ttggtacgcc acgggccatg aaaaagaatc tgctcgcggc
    10921 catcctgctg tccgcggcgt gcggatcgaa tccgccgccc acgaccacga ctccttcgac
    10981 cggcaccggc accgagaccg cgtcgtcgac gacctcggag caaacgagcg gcgcacagcc
    11041 ggcatcggtt tcgatgagca cgaagggcaa gcccgagtac ggcacgttcg gtttcgacac
    11101 gaacggcatg gacaagaaga cggcccccgg ccagagcttc tacaacttcg cgaacggcgg
    11161 ttgggtgaag gccacgccga ttccggcgga caagtcgaac tacggcatgt tcacggtgct
    11221 ctccgacaag agcgacgagc gcacgaagga gatcatcctc aacgcgaagg gcgcgcaggg
    11281 caccgacgcg cagcgcatcg ctgacttcta cggcagcttc atggacgagg ccgcgatcga
    11341 gaaggccggc atcgagccga tcaaaggcga cctcgcgaag atcgacgcga tcaaggacaa
    11401 gaacgcgctc gtgctcgcgt tcgcggcgaa ttcgcgccag atggacaaca cgccgttcga
    11461 gacgggcgtc gcgcaagacg acaaggatcc cgagcactac atcgcggtcc tcggtcaagg
    11521 cggcctcggc cttccggatc gcgacatgta cgacgcgaag aacaagcagt tcgagccgat
    11581 gcgtcagggc tacaagaagt atctcgagaa catgctgacg ctcgccggcg cgaaggacgc
    11641 ggcgaagaag gcggctgcgg tctacgcgct cgaggagaag atcgcgaaga cgcactggac
    11701 gcgcatccag aaccgcgacc cgcagaagac gtacaacaag atgacgatgg ccgagctgca
    11761 gaagctcgct ccatcggtcg actggaagtc gtggtcgaag gttgtcggct acgagggcca
    11821 gaccgcgatc aacgtcaacc agccgacggc gatcaccggc atcgcgaagc tcgtgaagag
    11881 cgagtcgctc ggcgtgtgga aagactacat gacggtgcac ctcgtctcgg acgcggcacc
    11941 gttcctctcg aagaagttcg tcgacacgcg cttcgagttc tatggcaaga cgctctcggg
    12001 cacgccggag ctcaaggctc gctggaagcg cggcgtcgac tacgt
//
LOCUS       DATLBZ010000328         3474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4840204, whole genome shotgun sequence.
ACCESSION   DATLBZ010000328 DATLBZ010000000
VERSION     DATLBZ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4840204"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VMZ53_17200"
     CDS             complement(<1..944)
                     /locus_tag="VMZ53_17200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30250.1"
                     /translation="MDDKPQTFGAYLLHEQIGSGGMASVHRAELRGADGTTKEVALKR
                     MLPEAVVQRQLRLAFRHEARLMRYLDHPNIPKTYDAGNYRETEFISMEYIGGPTLRDV
                     FAHSGKTVGFVPEPVALNIVAQLCDALDYAHNLRDETGKPLNIIHRDVTPSNLILSKT
                     GVVKLIDFGLAKAQLPRSPESNAGTIKGKLNYAAPEYLDGDQLDARMDIWALGVVLWE
                     LLTSRRLFDGPTPLETLTRIRKMPIPRPSIGNPRISEGLDNIVATALQRDPEKRWQTA
                     AQMGAAARTLIQRPESYCENRHVIEWVEWLLTLPPGTTV"
     gene            complement(949..2586)
                     /locus_tag="VMZ53_17205"
     CDS             complement(949..2586)
                     /locus_tag="VMZ53_17205"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30251.1"
                     /translation="MRNAIQTSSLLLVIATVVACTAAPPPAPSLTVTSPERGMLQNSA
                     GPVMVKGTALPGDDGAAVTSVKVNGTRASVGADGSFSVVVDAPPGALLLETVATSSEG
                     GTVTDARAVQTGEVRAVGTSVERGVTATLSAEAFARLATAAGPIVKSTDFNKLLAPMQ
                     PMANLGDSLANVKLSITKLTLGDVKFTLVPTDGGLQFSAELTGLSVAAKADYAGTFVP
                     DGSTSVNASADTVSISGTLVVTPAGTAGFTAKLASPTVRTTNLKLQASGLVGDVLSVL
                     QSNLSSTIQRIATTSTEKAIQPLLINALGALAGPQSLDVLGKKIELQVSPSAVTFSRA
                     GALVAMNVAVKIAGSEGSPGYVFTPNGTPKLDVSTGVQLALADDLVNEMLAEVHALGL
                     LDLRIQEDFGIFDEAHFRLALPPMISANNEDGTMRLVIGDMIASFSNHGTPVIGAAVN
                     AQVDVAVEQGTADEEIALKFGKIRVFVNPLDKDANMDDVENAELKLAATAGIGVQLDS
                     MKQFLVKLPVPAIAGIQLDNLSLHADSGYVVASGAIR"
     gene            complement(2612..>3474)
                     /locus_tag="VMZ53_17210"
     CDS             complement(2612..>3474)
                     /locus_tag="VMZ53_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30252.1"
                     /translation="AKAKAEAEDEADDADDDEDADDDEDADDDADEDEVAAIDDDEDE
                     ETDEAAVASVRINSNALTPRYARDWHIAAGPYVWASSVDANVSLGSESVGAAIGFIDM
                     QRHAKFGAPLLVEARYRRFAVTADATYAVVGINGTREIGPVMLSVDGTVKSLQFDGAA
                     GYMVLGGDSSLVSLEARAGIRYQRTAISGSLAVEGMPGEPVQMVSASRDAVAGARAFV
                     RPLPRLMFIGTADVGLFGSSARTWSAAADASVRVSHFLFSLGYRTMTTDSPYVSVRMH
                     GPRIAAQIVF"
BASE COUNT          553 a         1152 c         1134 g          635 t
ORIGIN      
        1 acggtggttc cgggcggcag ggtcagcaac cactcgaccc attcgatgac gtgccggttc
       61 tcgcagtagc tctcgggtcg ctggatcaac gtgcgcgccg cagcgcccat ctgcgccgca
      121 gtctgccacc gcttctccgg atcacgctgc agcgcggtcg ccacgatgtt gtcgagccct
      181 tcggagattc gtggatttcc gatcgacggc cgcgggatgg gcatcttgcg gatgcgcgtt
      241 agcgtctcga gtggggtcgg cccgtcgaac aggcggcgac tcgtgagcag ctcccacaac
      301 acgacgccga gcgcccagat gtccatccgc gcatcgagct gatcgccgtc gaggtactcc
      361 ggcgctgcgt agttgagctt gcctttgatc gttcccgcgt tgctctcggg ggagcgcggt
      421 agctgcgcct tcgccaagcc gaagtcgatg agcttgacga cgccggtctt cgacaagatg
      481 aggttcgacg gcgtgacgtc gcgatggatg atattgagcg gcttgcctgt ctcatcgcgc
      541 aagttgtgcg cgtagtcgag cgcgtcgcag agctgcgcca cgatgttgag cgcgaccggc
      601 tctggaacga agccgacagt cttgccgctg tgcgcgaaca catcgcggag cgtcggtccg
      661 ccgatgtact ccatcgagat gaactcggtc tcgcggtaat tgccggcgtc gtacgtcttc
      721 ggaatgttcg ggtggtcgag gtaacgcatc agccgcgcct cgtggcggaa cgcgagcctc
      781 aactgccttt ggacgacggc ctcgggcagc attcgcttga gcgcgacctc tttcgtcgtc
      841 ccgtccgctc cccgcagctc ggcgcgatgg accgacgcca tgccacccga cccgatctgc
      901 tcgtgcagca gatacgcgcc gaaggtctgc ggcttgtcgt ccacagcctc atcgtatcgc
      961 gccggacgcg accacgtacc cgctgtctgc atggagcgac aggttgtcga gctggatgcc
     1021 cgcgatcgcc ggcaccggca gcttgaccag gaactgcttc atcgaatcga gctgcacgcc
     1081 gataccagca gtcgcggcga gcttgagctc ggcattctcg acgtcgtcca tgttcgcgtc
     1141 cttgtcgagc ggattcacga agacacgaat ctttccgaac ttcagcgcga tttcttcgtc
     1201 ggcagtgccc tgctcgaccg cgacatccac ttgcgcgttg acggctgcgc cgatgaccgg
     1261 cgttccgtga ttcgagaatg acgcgatcat gtcgccgatg acgaggcgca tcgtaccgtc
     1321 ctcgttgttc gcgctgatca ttggcggaag cgcgagcctg aagtgcgctt cgtcgaagat
     1381 gccgaagtcc tcctggatgc ggaggtcgag gagcccgagt gcgtgcacct ccgcgagcat
     1441 ctcgttgacg aggtcgtccg cgagcgcgag ctgcacgccc gtgctgacat cgagcttcgg
     1501 cgtgccgttc ggcgtgaaga cgtatcctgg gctgccttcg ctacccgcga tcttgaccgc
     1561 aacgttcatc gcgacgagcg cgcctgcgcg gctgaacgtc accgcgctcg gcgatacttg
     1621 gagctcgatc ttcttgccga gcacgtcgag cgactgcgga ccggcgagag cgccgagcgc
     1681 gttgatgagc aacggctgga tcgccttctc ggtgctggtc gtcgcgatgc gctgaatcgt
     1741 cgagctcaag ttgctctgga gcacagagag cacgtcaccg acgagacccg acgcctgcag
     1801 ctttagattc gtcgtgcgca ccgttgggga tgcgagcttc gcagtgaaac ccgccgtacc
     1861 tgccggcgtg acgacgagcg tgcccgagat cgagaccgta tcggcgctcg cgttcaccga
     1921 cgtcgagccg tcgggtacga acgtgccggc gtagtcggcc ttcgcagcga cgctgaggcc
     1981 cgtgagctct gccgagaact gcagcccgcc gtccgtcggc acgagcgtga atttcacatc
     2041 gcccagcgtg agcttcgtga tcgagagctt cacgttcgcg aggctgtcgc cgaggtttgc
     2101 catcggctgc atcggggcga gcagcttgtt gaagtcggtc gacttcacga tggggcccgc
     2161 cgccgtcgcg agacgcgcga acgcttccgc cgatagtgtc gcagtcacgc ctcgctcgac
     2221 gctcgtgccg acggcgcgaa cctcgccggt ctgcacagcg cgcgcatccg tcaccgtgcc
     2281 gccctcgctc gacgttgcga ccgtctcgag cagcagcgcg cccggaggag cgtcgacgac
     2341 gacactgaac gagccgtccg cgccgaccga cgcgcgcgtg ccgttgacct tgacgctggt
     2401 gacggcagca ccgtcgtcgc ctggcaacgc cgtgcctttg accatgaccg gtcctgcgct
     2461 gttctgcagc atgccgcgct cgggagacgt gaccgtcagc gacggcgccg gtggtggtgc
     2521 ggcggtgcac gccacgaccg ttgcaatgac gagcagaaga gatgacgtct ggatggcgtt
     2581 gcgcatgatg gctgtccttt cggcggagag atcagaagac gatttgggct gcgattcgcg
     2641 gaccgtgcat ccgcaccgag acgtacgggc tatccgtcgt catcgtccgg tagccgagcg
     2701 agaagagaaa gtggctcacg cgcacgctcg cgtcggccgc ggcagaccac gtgcgcgctg
     2761 atgatccgaa caagccgaca tcggcagtac cgatgaacat gaggcgcgga agcggccgaa
     2821 cgaatgcgcg agcacccgcg acggcgtcgc gcgatgcgct caccatttgc accggctctc
     2881 cgggcatccc ctcgacggcg agcgaccccg aaatcgcggt gcgttggtac cgaatgccag
     2941 cgcgcgcctc gagcgacacc agcgagctgt cgccgccgag aaccatgtag cccgcggctc
     3001 cgtcgaattg cagcgacttc acggtgccgt cgaccgacag catcacgggc ccgatctcgc
     3061 gcgtgccgtt gatgccaacg accgcgtacg tcgcgtcggc cgtcaccgcg aaccggcgat
     3121 aacgcgcttc gacgagcagc ggcgcgccga atttcgcatg gcgttgcatg tcgatgaagc
     3181 cgatagcggc gccgacgctc tcggaaccga gcgagacgtt cgcgtcgacc gacgacgccc
     3241 acacgtaagg acccgccgcg atgtgccagt cgcgtgcgta gcgcggcgtc aacgcattgg
     3301 agttgatgcg aacggacgcg accgcggctt cgtcggtctc ctcgtcctcg tcgtcgtcga
     3361 tggccgcgac ctcgtcttcg tccgcgtcgt cgtccgcgtc ctcgtcgtcg tccgcgtcct
     3421 cgtcgtcgtc cgcgtcgtcg gcttcgtcct cggcctcggc cttggccttc gcgt
//
LOCUS       DATLBZ010000329         7035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8382842, whole genome shotgun sequence.
ACCESSION   DATLBZ010000329 DATLBZ010000000
VERSION     DATLBZ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8382842"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2463)
                     /locus_tag="VMZ53_17215"
     CDS             complement(<1..2463)
                     /locus_tag="VMZ53_17215"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HUS30253.1"
                     /translation="MTAAIGCSDDPSLEDYFPPLPPTGGPQQAFAGQVNDASQLIAGP
                     AASGMVGDFYIRNDKVSFIVQSPARVLGVIPQGGNVVDAVLADGTQVDQFGELGLIYL
                     VGRTCDPDRIEIVRDGSKGGAAVLRAIGKSANNDFINLKAIGVFPVEQGVDPDIDDGV
                     QCATTYVLAPGSTALEVYHSLFNEGPDDINGPVGILADSGGNTEAWTNTRGFERADVT
                     AIAALGNPTPSDYVIYQAPGVAYGVIPRDKIPTVHTHALLAGVSILLSGNSYLLEILQ
                     RDKYTLHMKAGSGFLAHVDLAVGRDANEIDTVWRAASASTGTQPTMESITGRVDWSGG
                     GAATGARVGIYVDGNGNGTLDEPEVETNDDFIPDDHAISYLDVKPDGTFSGYVPTTAG
                     NLLVRAEVKNVGRSQAAPVAANVTLTIPSPVKVDYQILDADTGMPIPGTLLVMGTHPA
                     FPDKRLFETYDRAPGVVQLQSCIRGTTVDVGDGVDAPLYLPAGGTYRVYATHGTEWSV
                     ASASVTGSAPVDLTFTLKHVVPSDGYLSTEWHVHMLGSPDSPVTAEERVRGAVANGIE
                     MFAVTDHDYVSDLQPTVEQLGLQSQLRVVSGIEVTPFAYGHYMTWPIAPDDSSANRGA
                     IDWARGAALDLAMTPGEIYDAMRARGGQMVQVNHPRGNGLSEFQAAFSRANVKYDYTQ
                     RTIYGDYQNASTPADWLRLPGESLWSDRFNGLEVWNNFSIADSNADGLRENRSIDRVM
                     RDWLSMLSLGMYVTPSGSSDTHTANADPMGMPRTFVRVADDSSAALLSPASVDAVLQT
                     QTGANGAPRDVIVTDGPMIDVRA"
     gene            2559..2831
                     /locus_tag="VMZ53_17220"
     CDS             2559..2831
                     /locus_tag="VMZ53_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HUS30254.1"
                     /translation="MSRMVKCAKYHEELPGLPYKPFQDALGQRIYDNISAKAWQDWIE
                     HSKRLVNEYRLDLTTKKAHEVLKEQCEDFLFGAGGSAPAEYVPEPK"
     gene            2846..3997
                     /locus_tag="VMZ53_17225"
     CDS             2846..3997
                     /locus_tag="VMZ53_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HUS30255.1"
                     /translation="MQNASALARYLPRIAAKIAWAPLGDWPTPISELQIDGAPLWVKR
                     EGDSSALYGGNKVRTLEAWLGHTRASGARRIWSIGAYGSNHVVATVLHAKRLGLEAAA
                     IVFPQPASEWAIENAGALIATGVPIVRLRTVIEVPFAGIAIAARAKWGKLIAELAAQS
                     PASRDLLSALVGDELARDSIVMPPGGATPIGTLGALSAVFELLEQVDAGVMPPPRRIV
                     LPVGSTCTTAGLVSGLALAHAIGAWRWPVPVVHAVRVTPWPVTSRVVILRLAEATLAR
                     IASLGGPRITLSQPRLVVDREEFGGGYGRITDRGREAIARFASLEDAPRLDGVYSAKA
                     AAALLRLHHEKRGPLLFWATKSSTLMQPPASNALRSAPRALARWLDAPT"
     gene            complement(3990..5555)
                     /locus_tag="VMZ53_17230"
     CDS             complement(3990..5555)
                     /locus_tag="VMZ53_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30256.1"
                     /translation="MDRQDIDALLVGALYGELTPADEARLTAHLDSHPGDRGALDDLK
                     SARAKVRESRIFELQAEPPQHVSALLLQEAHRRAPKKVVAEDGERRDSWFWRFARSFA
                     LHPAMAAAAMLIVVLGVSAIAVKNKSASFAPREAQPPAATQQETSETLAQAPSANDTT
                     AAGSAAALDEGAAFHAGLAEEGDLARAQGGEAEKKNEKNEESASQRWRSANDGKVAAA
                     PAKNKGIAVTTEQAQPKDLDNLRDERRAAESQLAKEQPERKKSAAKADSSAYGYDGTS
                     DDNYGAPSTGADRGALAGAGGGGAANGIVVGGAAPGASPKGNVAAGPAAATPAPVTTT
                     SPANRPYAQPPPPPPSADKASVAKQEPAKPTAPAAKSEAPSGATGSTVAKAPTASTAT
                     GTKTGSTTSTPTNDAKKVATQKPAEKSPPATAPAQQAQDPTVAWAKGQHASAVKLAKG
                     GDCAGAAKLALTVSQKAPDYYASFMASDRQLKSCKAYIDAERDKEAEKSAKSRAQKRV
                     NADEPAPSNESVK"
     gene            complement(5748..6062)
                     /locus_tag="VMZ53_17235"
     CDS             complement(5748..6062)
                     /locus_tag="VMZ53_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30257.1"
                     /translation="MPAAVVAKNNKKSIADCTSFQQNEKGDEAVEITIHNSCAIPVDC
                     SLSWRVVCAPDSKRRSVHDSSKKFSLTSGMEEKNEATATVCGDDGWSIDSIKWGCEPN
                     KD"
     gene            6242..>7035
                     /locus_tag="VMZ53_17240"
     CDS             6242..>7035
                     /locus_tag="VMZ53_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS30258.1"
                     /translation="MPSPLDVFRDDVLHGHVALITGGGTGICRGIAHAYAKLGADVCI
                     VSRKPDVLEKTAAELSAATGRKILAVAADVRNPDAIATAVATTVERFGKLDTIVNGAA
                     GNFLAPAAGLSPNGFKTVIDIDLVGTFNTARAAFEHLQKSGNGLVLNISATLHYHGTP
                     LQVHASAAKAGVDAVTKNLAVEWGRFGIRSCGIAPGPIGDTEGMRRLAPGEIKDKAMT
                     GIPAGRFGTIDEIAAAAVYLRSPAAAYVTGHIMVVDGGHCVSTPSL"
BASE COUNT         1121 a         2418 c         2251 g         1245 t
ORIGIN      
        1 agcacgcacg tcgatcatgg gaccgtcggt cacgatcaca tcgcgcggcg cgccgttcgc
       61 gcccgtctgc gtctgcagca cggcatcgac cgacgctggc gagagcagcg ccgccgacga
      121 atcgtccgca acccggacga atgtgcgagg catccccatc ggatcagcat tcgcggtgtg
      181 cgtgtcactc gagcccgacg gcgtcacgta catgccgagc gacagcatgc tgagccaatc
      241 gcgcatgacg cgatcgatcg agcggttctc gcgcaggccg tcggcattcg agtcggcgat
      301 cgagaagttg ttccagacct cgaggccgtt gaagcgatca ctccacagtg actcgccggg
      361 gaggcgcaac cagtccgccg gcgtcgacgc gttctggtag tcgccgtaga tcgtgcgctg
      421 cgtgtaatcg tacttcacgt tcgcgcgcga gaacgcggcc tggaactccg agaggccgtt
      481 gccgcgcggg tgattgactt gcaccatctg gccgccacgc gcacgcatcg cgtcgtagat
      541 ctcgcctggt gtcatcgcga gatcgagcgc agcgccgcga gcccaatcga tcgcgccgcg
      601 attcgccgac gagtcgtcgg gagcgatcgg ccacgtcatg taatggccat acgcgaacgg
      661 cgtgacctcg atgcccgaca cgacgcgcag ctggctctgc agcccgagct gttcgacggt
      721 cggctgcaaa tcggagacgt aatcgtgatc ggtgaccgcg aacatctcga tgccgttcgc
      781 caccgcgccg cgcacgcgtt cttccgccgt caccggcgag tccggcgagc cgagcatgtg
      841 gacgtgccac tccgtcgaca agtaaccatc gctcggcacc acatgcttga gcgtgaacgt
      901 gaggtcgact ggcgcgctgc ccgtcaccga tgccgacgcc accgaccatt cggtgccatg
      961 cgtggcgtag acacgatacg tgccgccggc cggcaaatag agcggcgcgt cgaccccatc
     1021 gccgacatcg accgtcgtgc cgcggataca gctctgcagc tgcacgacac caggcgcgcg
     1081 atcgtacgtc tcgaacaacc gcttgtcggg gaacgccgga tgcgtgccca tcacgagcag
     1141 cgtgccggga atcggcatgc ccgtgtctgc gtcgaggatt tggtagtcga ccttcacggg
     1201 gctcgggatc gtgagcgtca cgttcgcggc gaccggcgcg gcttgcgacc gacctacatt
     1261 cttgacctcc gcccgcacga gaaggttgcc tgcggtcgtg ggcacgtaac cgctgaacgt
     1321 gccgtcgggt tttacgtcga gatagctgat ggcgtggtca tcgggaatga agtcgtcgtt
     1381 ggtctcgacc tccggctcgt cgagcgtgcc gttgccattg ccatcgacgt agatgccgac
     1441 gcgcgctccc gtcgcggccc cgccgccgga ccaatcgacg cgcccggtga tggactccat
     1501 cgtcggttgc gttccagtcg acgcgctcgc tgcgcgccac accgtgtcga tctcgtttgc
     1561 gtcgcggccg accgcgagat cgacgtgcgc gaggaagccc gagccggcct tcatgtggag
     1621 cgtgtacttg tcgcgttgca ggatctctag caggtagctg ttgccggaca gcaagatcga
     1681 aacaccggcg agcagcgcgt gcgtgtgcac ggtcggaatc ttgtcgcgag ggatcacgcc
     1741 gtacgcgaca ccgggcgctt gatagatcac gtagtccgac ggcgtcggat tgccgagcgc
     1801 agcgatcgcg gtgacgtcgg cgcgttcgaa gccgcgcgtg ttcgtccacg cttcggtgtt
     1861 gccgccggaa tcggcgagga tgccgacggg accgttgatg tcatcggggc cttcgttgaa
     1921 cagcgagtga tagacctcga gcgccgtcga gcccggcgcg agcacgtatg tcgtcgcgca
     1981 ctgcacgccg tcgtcgatgt ctggatcgac gccctgctcg acgggaaata cgccgatcgc
     2041 cttgaggttg atgaagtcgt tgttcgcgct cttgccgatt gcacgcagca cggcggcgcc
     2101 gcctttgctg ccgtcgcgca cgatctcgat gcgatccgga tcgcacgtgc gaccgacgag
     2161 atagatgaga ccgagctcgc cgaactgatc gacctgcgtg ccatctgcaa gcaccgcatc
     2221 gacgacgttg ccgccctgcg ggatgacgcc gagcacgcgc gcaggcgatt gcacgatgaa
     2281 cgacaccttg tcgttgcgaa tgtagaagtc gccgaccatg ccgctcgctg cggggcccgc
     2341 gatgagctga ctcgcatcgt tcacctgtcc ggcgaacgcc tgctgcggtc ctccggtcgg
     2401 aggcaacggc gggaagtaat cctcgaggct cggatcgtca ctgcatccga tcgcggcggt
     2461 cacgagagcg aagcaaacaa ggtggcggca ctgcacgcgc gcatcgtaca gaagtggtcg
     2521 aaccatcagg tgcccgattt cgtggtatgc cgccgcccat gagccgcatg gtcaagtgcg
     2581 cgaagtacca cgaggagctg cccggtctcc cctacaagcc gttccaggac gcgctcggcc
     2641 agcgcatcta cgacaacatc tcggcgaagg cctggcagga ctggatcgag cactccaagc
     2701 gcctcgtcaa cgagtaccgc ctcgacctca cgacgaagaa agcgcacgag gtcctcaagg
     2761 aacagtgcga ggactttctg ttcggcgccg gcggcagcgc accggccgaa tacgttcccg
     2821 agccgaagta gccgttccgc gctgcatgca gaacgccagc gcgctcgcac gatacctgcc
     2881 gcgcatcgca gcgaagatcg cgtgggcccc gctcggtgat tggccaacgc cgatcagcga
     2941 gctgcagata gatggcgcgc cgctctgggt caaacgcgaa ggcgactcca gcgcgctcta
     3001 cggcggcaac aaggttcgca cgctcgaggc gtggctcggt cacacgcgtg cgagcggcgc
     3061 acgtcgcatt tggtcgatcg gcgcgtacgg atcgaatcac gtcgtcgcga ccgtgctcca
     3121 cgcgaagcgc ctcggtctcg aagccgcggc gatcgtgttt ccgcaaccgg cgagcgagtg
     3181 ggcgatcgaa aatgcaggcg cgctgatcgc gacgggtgtg ccgatcgttc ggttgcgcac
     3241 cgtaatcgaa gttccgttcg cgggcatcgc gatcgcggca cgcgcgaagt ggggcaagtt
     3301 gatcgcagag ctcgccgcgc aatcgcccgc atcgcgcgac ctcctcagcg cactcgtcgg
     3361 tgatgagctc gcgcgcgatt cgatcgtgat gccacccggc ggcgcgacac cgatcggcac
     3421 gctcggcgcg ctgtcggccg tgttcgagtt gctcgagcaa gtcgacgcgg gcgtgatgcc
     3481 gccgcctcgc cggatcgtgc tccccgtcgg cagcacgtgc acgacagccg gcctcgtctc
     3541 ggggctcgcg ctcgcgcacg cgatcggcgc gtggcgttgg ccggtgcccg tcgtccatgc
     3601 agtacgagtc acaccgtggc cggtcacgtc acgcgtcgtc atcctgcgcc tcgcggaggc
     3661 aacgctcgcg cgcatcgcat cgctcggcgg cccgcgcatc acgctctcgc agccacgcct
     3721 cgtcgtcgat cgcgaggagt tcggcggcgg ctacggccgc atcaccgatc gcggacgcga
     3781 ggcgatcgcg cgctttgctt ccctcgaaga cgcgccgcgc ctcgatggtg tttacagcgc
     3841 taaagcggcg gctgcgctgc tgcggctcca ccacgagaag cgcggcccgc tgctgttctg
     3901 ggcgacgaaa tcatcgaccc tgatgcaacc gccggcgagc aatgcgctgc gcagtgcgcc
     3961 gcgtgcactc gcgcgttggc tcgacgcgcc tacttgacgc tctcgttcga cggcgccggt
     4021 tcgtcggcgt tcacacgctt ctgcgcgcgg ctcttcgcgc tcttctcagc ttccttgtcg
     4081 cgctccgcgt cgatgtacgc cttgcagctc ttcagctggc gatcgctcgc catgaagctc
     4141 gcgtaatagt cgggcgcctt ttggctcacg gtgagtgcga gcttcgcagc gccggcgcaa
     4201 tcaccgccct tggcgagctt caccgcactc gcgtgctggc ccttcgccca cgcgaccgtc
     4261 ggatcctgcg cttgctgcgc aggtgccgtc gccggcggtg acttctcagc aggcttctgc
     4321 gtcgcgacct tcttcgcgtc gttcgtcggt gtgctcgtgg tgctgccggt cttcgtgccg
     4381 gtcgcggtcg atgccgtcgg cgccttcgca accgtcgagc ctgtagctcc cgagggcgct
     4441 tccgattttg ccgcaggcgc ggtcggcttc gccggttctt gcttcgcgac gctcgccttg
     4501 tccgctgacg gaggcggagg aggcggctgc gcgtacggac gattcgctgg cgacgtggtg
     4561 gtgaccggcg ctggagtcgc agcagccgga cccgcagcaa cgttgccctt gggcgacgca
     4621 cccggcgcgg cgccaccgac gacgatgccg ttcgctgcgc caccgccacc cgcgcccgca
     4681 agcgcgccac ggtcggcacc cgtcgacggt gcgccgtagt tgtcgtcgct tgtgccgtcg
     4741 tagccgtagg cgctgctgtc agccttcgcc gcgctcttct tgcgctccgg ttgttccttc
     4801 gcgagctgtg attcagcagc acgacgctcg tcgcgcaggt tgtcgagatc ttttggctgc
     4861 gcctgctcgg tcgtaacggc gatgcctttg ttcttcgcgg gcgccgcggc gaccttgccg
     4921 tcgttggccg agcgccagcg ctgagatgcg ctctcttcgt tcttctcgtt cttcttctct
     4981 gcttcgccgc cctgcgcacg cgcgaggtca ccctcttccg cgagccctgc gtgaaaggca
     5041 gcaccttcat cgagcgcggc ggctgaaccc gcggcggtcg tgtcattcgc ggacggcgct
     5101 tgcgcaagcg tctcgctggt ctcctgttgc gtcgcggccg gcggctgcgc ttcgcgaggt
     5161 gcaaagctcg cactcttgtt cttcacggcg atcgcgctca cgccgagcac gacgatgagc
     5221 atcgcggctg cggccatcgc gggatgcagc gcgaacgagc gcgcgaaccg ccagaaccag
     5281 ctgtccctcc gctcgccatc ttccgcgaca accttcttgg gtgcccggcg atgggcttct
     5341 tggagcagca gcgccgacac gtgctgcggc ggctcggcct ggagctcgaa gatccgactt
     5401 tcgcggacct tcgcgcgcgc tgacttcagg tcatcgaggg cgccccggtc acccgggtgc
     5461 gagtcgaggt gcgccgtcag ccgggcctca tcagcgggcg ttagctcgcc gtagagggcg
     5521 cctaccagca gcgcgtcgat gtcctgacga tccatgacct tcgtgtcctt atactacgtg
     5581 tgtcttaccc gagcgacgaa ccaggctttc cgaggacttg gcacctactt cgtgccggtc
     5641 tctcgggcca ttcggttcgt agcttcgaga ttacaaacgt aggactacgc ggatggatct
     5701 aacggcctgc gaaaagtggt ctacatccca cgtcgcaggg gcaaccccta gtctttgttg
     5761 ggctcgcaac cccacttgat cgagtcgatc gaccagccgt catcgccgca gacggtcgca
     5821 gttgcctcgt tcttctcctc cataccggac gtcagggaga acttcttgga gctgtcgtgg
     5881 accgagcgac gcttcgagtc cggcgcacac acgacgcgcc acgacagcga gcagtcgacc
     5941 gggatcgcgc agctgttgtg aatcgtgatc tccaccgcct cgtcgccctt ctcgttctgc
     6001 tgaaacgaag tgcagtcggc gatcgacttc ttattgttct tcgcgacgac agcagcgggc
     6061 acgcagatcg acacaacgag ggcgcagatc gcccctcgcg accaagaagg acgcaacatc
     6121 ggacaagcct ccggcaattg ggtgacgcac gcgccttgcg gcagcagcgt cggttcaaag
     6181 cactagacac cggtcccgcc gcgaagtttc ggatttcgac caagccatgc aaaatccgca
     6241 aatgcccagc cctctcgacg tcttccgcga cgacgtcctc cacggtcacg tcgccctcat
     6301 cacaggcggt ggaaccggaa tctgccgcgg tatcgcacac gcctacgcaa agctgggcgc
     6361 cgacgtctgc atcgtgagcc gcaagcccga cgtgctcgag aagacggccg ccgagctctc
     6421 ggccgccacg ggccgcaaga tcctcgcggt cgccgccgac gtccggaacc cggacgctat
     6481 cgccacggcg gtcgccacga ccgtcgaacg gttcggcaag ctcgacacga tcgtcaacgg
     6541 cgcggccggt aactttctcg cgcccgctgc cggcctctcg cccaacggct tcaaaacggt
     6601 catcgatatc gatctcgtcg gcacgttcaa cacggcgcgc gccgcgttcg agcatctaca
     6661 aaagtccggc aacggcctcg tgctcaacat cagcgcgacg ctccactacc acggcacgcc
     6721 gctccaggtt cacgcatccg ccgcgaaggc cggcgtcgac gccgtcacca agaacctcgc
     6781 cgtcgagtgg ggccgcttcg gcattcgctc gtgcggcatc gcgccaggcc cgatcggcga
     6841 caccgaaggc atgcgccgcc tcgcgcccgg cgagatcaaa gacaaggcga tgaccggaat
     6901 cccggccggc cgcttcggca caatcgacga aatcgccgcc gccgccgtct acctgcgctc
     6961 gcccgccgcg gcgtacgtga caggccacat catggtcgtc gatggcggcc actgcgtgtc
     7021 gacgccctcg ctgat
//
LOCUS       DATLBZ010000330         5897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8383053, whole genome shotgun sequence.
ACCESSION   DATLBZ010000330 DATLBZ010000000
VERSION     DATLBZ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8383053"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VMZ53_17245"
     CDS             <1..1155
                     /locus_tag="VMZ53_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30259.1"
                     /translation="LSEGSIAAIIGNGARGEASGPASVSVGEGKSLVKLSAGTLAIEA
                     KDADAIANVPGGLITTAKGGAATATVDAKGATTIDAHRGATTIETTKGTETVAEGESI
                     TITAKGEVERAAPPPKRTVAVIAAGESPVIHDGAAPTPIRVTFETCKGPGTVEVAKDR
                     SFKRVIARSSGEGAANVLVPLGSFSYRVKCAGKGASGTIRVAKDSGRTPLPKAAAKTS
                     VEMDGREYTILYQNLLPELTLSWRTAPKKASYTFVVKPQAGAEKRFASATPKLTLKPG
                     ELREGSYTAWVEPAGGAKSEQGKLIIEFDNAAASASIDDVAPVDGKTRVKGTVIEGST
                     VSVNGTTVELDRHRRFQTDLAPADGEDGVAVRIANSKAGIHYYVMRTAPH"
     gene            1173..2816
                     /locus_tag="VMZ53_17250"
     CDS             1173..2816
                     /locus_tag="VMZ53_17250"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30260.1"
                     /translation="MAAVIGQTVGNYKIVSEIGKGGMGVVYQAEHTLLGKKAAIKLLR
                     SDVPTDQVQRFFNEAKAAATLRHPGLVDVIDFGHAKDGSAFIVMELLQGESLAERLER
                     DPRLPVPIACTIARHVANALAVAHNEGIIHRDLKPGNIFLLPDAESPAGVRTKVLDFG
                     IAKLQRDRDDKSAQTHSGAVIGTPRYMSPEQCKNAREVDGRADIYSLGCILFEMLLGV
                     APFDYDSWAELVSAHLKETPPRPSELDPAIPKDVETLVTKMLSKKPTDRHRSMEELAQ
                     SIEVILREHAQDWPVRVTPVSGVRRIQSKQHLDETVPASSDRLTPVRLDSGTDDTLPP
                     TPPPGTLKPASPSEPGTTTAPHRPTPQPESASPAAPPAKKLPWVAIGIAAGGAIALAG
                     AIVLVLANKSSKQPDETAYIVVKEGGSDDSPHGSATVQTPPPIVDAAVAEIAVDAAVK
                     PTKPTGNDQAAALTRTFGKQTNAIAACIRQHADLAQTKEDLSVRIQIDTQGKVTTATV
                     LPASVASTALGTCIADVAKSTDFGAQPKPASFRVPIHTR"
     gene            complement(2918..3838)
                     /locus_tag="VMZ53_17255"
     CDS             complement(2918..3838)
                     /locus_tag="VMZ53_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30261.1"
                     /translation="MRGLVVPALVLIAGCGASTGGPTLGGPCDPENPCNTGEACDMTA
                     DGGPICITGEGDLDGDGIPNLKDFCQHKQGGASDEDLDGIGDDCDKCPIAAPRPTPDP
                     DGDNVESPCDPDPHEPGDEILLFESFSSTTIDARWKATTASVWKAVGGEVIATAPATG
                     PQDYLTTLVAGKNNISIEASYRIDKIDTTTSPVQHIVAMYMSDPRPAGVAQASCGVSK
                     TDGGQSELVLVETNQGAMNQLVSGAFDTAALYRAGGYVSGTRAACSVIANNNPLGTVQ
                     ASITPDQLSSISLTAKAATARFRYVLVVGH"
     gene            3956..>5897
                     /locus_tag="VMZ53_17260"
     CDS             3956..>5897
                     /locus_tag="VMZ53_17260"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HUS30262.1"
                     /translation="MNMRAFALPLALIGAAVLAAGCDDDDGGGPQDHVVAKTTQLVQY
                     SGCSDLEADLKQMVKWEVFADIDQADHWGWGGEDAAGEGAGGGASPDNGGGRQEGVDF
                     SGTNNQEQGVDEADFVKTDGYHIYTLNGNRLHIMGVPNFGELTAESVTQIEGYPQQML
                     LDAAQNRIVVFSWIDTYSLPEGHPLRQLVGYKDDDSDNWYWRIKQLSKITVLDITDRS
                     HPALVRELFFEGWYQTARKVDSTIRMASYSMIDPAIMWGWWQIYNDNNHNKTATKAVV
                     AAYINSLHLADFIPQIYVRTPDGHFATNSLSEGSCRSFYRPTDSHARGISSILSLDLL
                     GNTLHWDADHIISNWSTFYASQDRLVLAEPAHDWWWYWYWQNDPDQLNIHSFDISVPG
                     QTRYIGSGRVDGQIVDQFSLDEEGGAIRVATTTGLWGRWWLPAEEREEMENHVWVLGQ
                     SGNALEPIGHIGGIAKGERIMTSRMQGNKGYLVTFQYVDPLITLDLSDNTHPRVVGEL
                     KVPGFSTYLHPIADGKLLSIGIDNTAHWRTQISMFDVSDFAHPTLQEALPVEAQNTWG
                     WSEALYDHHAFQYWAPKKLLAIPQSTYAYQNWNGSYYYQYLSQLVLVNVDDTAGLSIH
                     GRIDHSSFYNADPNNYWQWTDIR"
BASE COUNT         1121 a         1991 c         1850 g          935 t
ORIGIN      
        1 ctcagcgagg gcagcatcgc tgcgatcatc ggcaatgggg cgcgcggcga ggcgagcggc
       61 ccggccagcg tgagcgtggg cgagggcaag tcgctcgtga agctgtcggc gggaacgctc
      121 gcgatcgaag ccaaggacgc cgacgcgatt gcgaatgtgc cgggtgggct catcacgaca
      181 gcgaagggcg gcgcggcgac agcgactgtc gatgcgaagg gcgcgaccac gatcgatgcg
      241 caccgcggcg cgacgacgat cgagacgaca aagggcaccg agaccgtcgc cgagggcgag
      301 tcgatcacga tcacggccaa gggcgaagtc gagcgcgcgg caccaccgcc gaagcgcacc
      361 gtcgctgtga tcgcggccgg cgagtcgccc gtcattcacg atggcgccgc accgacgccg
      421 attcgcgtca cgttcgagac gtgcaagggg ccaggcaccg tcgaggtcgc gaaggatcgc
      481 agcttcaagc gggtgatcgc gcggtcgtcg ggggaaggag cagcgaacgt gctcgtgccg
      541 ctgggctcgt tcagctaccg cgtgaagtgc gcgggcaaag gcgcgtcggg aacgattcgc
      601 gtcgcgaagg actcgggccg cacgccactg ccgaaggccg ccgcgaagac gagcgtcgag
      661 atggacggcc gcgagtacac gatcctctat cagaatctgc tccccgagct gacgctgtcg
      721 tggcgtaccg cgccgaagaa ggcgagctac acgttcgtcg tcaagccgca ggcgggcgcc
      781 gagaagcggt tcgcgagcgc gacgccgaag ctgacgctga agcccggcga gctgcgcgaa
      841 ggcagctaca cggcgtgggt cgagcccgcg ggtggcgcga agtccgagca gggcaagctc
      901 atcatcgagt tcgacaacgc agcggcctcg gcgtcaatcg acgacgtcgc gcccgtcgac
      961 ggcaaaacgc gcgtgaaggg caccgtgatc gagggctcga ccgtcagcgt caacggcacc
     1021 accgtcgagc tcgaccggca tcggcggttc cagacggacc tcgcacctgc agacggtgag
     1081 gacggcgtcg cggtgcgcat cgcgaacagc aaggccggta ttcactacta cgtgatgcgc
     1141 acggccccgc actagcttac gatctagcga gcgtggcagc ggtgatcggt cagacggtcg
     1201 ggaactacaa gatcgtttcc gagatcggga agggcggcat gggtgtcgtc tatcaagcgg
     1261 agcacacact gctcggcaag aaggccgcca tcaagctgct gcgctccgac gtaccgaccg
     1321 atcaagtcca gcgcttcttc aatgaagcaa aggcggccgc gacgctgcgt catccgggtc
     1381 tcgtcgacgt gatcgatttc ggccacgcga aggacggcag cgcgttcatc gtgatggagc
     1441 tcctgcaagg cgagtccctt gccgagcggc tcgagcgcga ccctcgcctg cccgttccga
     1501 tcgcgtgcac gatcgcgcgt cacgtcgcca atgcactcgc ggtcgcgcac aacgaaggca
     1561 tcattcatcg cgacctcaag cccggaaata tcttcctgct tcccgatgcc gagtcgcccg
     1621 ccggcgttcg cacgaaggtc ctcgattttg gcatcgcaaa gctgcagcgc gatcgcgacg
     1681 acaaatccgc gcagacgcac tccggcgccg tcatcggcac gccgcgttac atgtcgcccg
     1741 agcaatgcaa gaacgcgcgc gaggtcgacg gccgcgccga tatctactcg ctcggctgca
     1801 tcctgttcga gatgctgctc ggcgtcgcgc cattcgatta cgacagctgg gcggagctcg
     1861 tgtccgcgca tctcaaagag acgccaccac gtccgtccga gctcgatccg gcgatcccga
     1921 aagacgtcga gaccctcgtc acgaagatgc tctcgaagaa gccgaccgat cggcatcgct
     1981 cgatggaaga gctcgcgcaa tcgatcgagg tgatcctgcg cgagcacgcg caagactggc
     2041 ctgttcgcgt cacgccggtg tcgggcgtcc gccgcatcca gtccaagcaa cacctcgacg
     2101 agacggtgcc cgcatcgagc gatcgcctca ccccagtcag gctcgacagc gggaccgacg
     2161 acacgcttcc gccgacgccg ccgcccggca cgctcaagcc cgcatctcca tccgagccag
     2221 gcacgacgac cgcgcctcat cgcccaacgc cgcagccgga atccgcatcg cccgctgcac
     2281 cgcccgcgaa gaagctaccg tgggtcgcaa tcggcatcgc cgctggtgga gcgatcgcgc
     2341 tcgcgggcgc catcgtgctc gtgctcgcga acaagagcag caaacagccc gacgagaccg
     2401 cgtacatcgt cgtcaaagaa ggcggctccg acgactcgcc gcacggcagc gccacggtgc
     2461 aaacaccgcc gcccatcgtc gacgcggcag tcgcggaaat cgctgtcgac gcagcggtca
     2521 agccgacgaa gcctaccggg aacgatcaag cggccgcgct cacgcgcacg ttcggcaaac
     2581 agacgaatgc gatcgcggcc tgcatacgcc agcacgccga cctcgcgcag acaaaagaag
     2641 atctatcggt ccgcattcag atcgatacgc aaggcaaggt cacgaccgcc acggtgctgc
     2701 cggcatcggt cgcgagcacc gcgctcggga cgtgcatcgc cgacgtcgcg aagtcgaccg
     2761 acttcggcgc gcagcccaag cccgcctcgt tccgcgtgcc gatccacacg cgctaataat
     2821 caagtccgac cgaatttctg gtcagaccac aaattcagtc cgacctaatt tcctactgga
     2881 aatttccgct agaaaaatac gtcggactcg atttccgtta gtggccgacc acgagcacgt
     2941 atcggaagcg cgccgtggcc gctttggcgg tcagcgagat cgatgacagc tggtccggcg
     3001 tgatcgacgc ctgcaccgtg ccgagcggat tgttgttcgc gatcacgcta catgcggcgc
     3061 gcgtgccgga cacgtagcca cccgctcggt agagagccgc ggtgtcgaac gcgccggaca
     3121 cgagctggtt catcgcgcct tggtttgtct cgacgagcac gagctcgctc tgcccgccgt
     3181 ccgtcttgct cacgccgcac gatgcttgcg caacgccggc tgggcgcggg tcactcatgt
     3241 acatcgcgac gatgtgttgg actggcgacg tcgtcgtgtc gatcttgtcg atgcgatagc
     3301 tcgcttcgat cgagatgttg ttcttgccgg ccacgagtgt cgtcaggtag tcctgtggtc
     3361 ccgtcgcggg tgcggtcgcg atcacttcgc cgcccaccgc tttccagacc gacgccgtcg
     3421 tcgctttcca gcgcgcatcg atcgtcgttg acgagaagct ctcgaacagc aggatctcgt
     3481 caccgggctc gtgcggatct ggatcgcagg ggctctcgac gttgtccccg tccggatcag
     3541 gcgtcgggcg cggcgcggcg atcgggcact tgtcgcaatc gtcgccgatg ccgtcgaggt
     3601 cctcgtcgga ggcgccgccc tgcttgtgct ggcagaagtc cttgaggttc gggatgccgt
     3661 ctccgtcgag gtcgccttcg cccgtgatgc agatcgggcc accgtccgcg gtcatgtcgc
     3721 aggcctcgcc cgtgttgcac gggttctcgg ggtcgcacgg gccgccgagc gtgggaccac
     3781 ccgtgctcgc gccgcagcca gcgatcagga ccagggccgg aacgacgagg cctcgcacgg
     3841 ttcgatcatc tcagattttc gagtcactca tccccccaca cgcgagacat ctgacgtgga
     3901 ccgtgtggca tctcgcgagg ttagcagtgg caatgatctt gctgaaccgg gtggcatgaa
     3961 catgcgcgcc tttgccctcc cgctcgccct gatcggagcc gcggttctcg ccgccggctg
     4021 tgatgacgac gacggcggtg gtccccagga tcacgtcgtc gcgaagacca cgcagctcgt
     4081 ccagtactcg ggctgtagcg acctagaggc cgacctcaag cagatggtga agtgggaagt
     4141 gttcgccgat atcgatcagg cggaccactg gggctggggc ggcgaagatg ccgcaggtga
     4201 aggcgccggt ggtggcgcat cgcccgacaa cggtggtggg cgccaggaag gcgtcgactt
     4261 ctccggcacg aacaaccagg agcaaggcgt cgacgaagcc gacttcgtga agacggacgg
     4321 ctatcacatc tacacgctca acggtaatcg cctccacatc atgggcgtcc cgaacttcgg
     4381 cgagctcacc gcggagagcg tcacgcagat cgaaggctac ccgcagcaga tgctgctcga
     4441 cgccgcgcag aaccgcatcg tcgtcttctc gtggatcgac acctactcgc tccccgaagg
     4501 ccatccgctt cgccagctcg tcggctacaa ggacgacgac agcgacaact ggtactggcg
     4561 catcaagcag ctgtcgaaga tcaccgtcct cgacatcacc gatcgctcgc accccgcact
     4621 cgttcgcgag ctgttcttcg aaggctggta ccagaccgcg cgcaaggtcg actcgacgat
     4681 ccgcatggcc agctactcga tgatcgatcc cgcgatcatg tggggctggt ggcagatcta
     4741 caacgacaac aatcacaaca agaccgccac gaaggcagtc gtcgccgcgt acatcaactc
     4801 gctgcacctc gcggacttca tcccgcagat ctacgtccgg actccggacg gtcacttcgc
     4861 gacgaacagc ctctcggaag gctcgtgccg ctcgttctat cgtccaaccg acagccacgc
     4921 gcgcggcatc tcgtcgatcc tttcgctcga tctcctcggc aacacgctgc actgggatgc
     4981 cgaccacatc atctcgaact ggtcgacgtt ctacgcgtcg caagaccgcc tcgtcctcgc
     5041 cgagcccgcg catgactggt ggtggtactg gtactggcag aacgacccgg accagctgaa
     5101 catccactcg ttcgacatct cggttcccgg ccagacgcgc tacatcggct cgggtcgcgt
     5161 cgacggtcag atcgtcgacc agttctcgct cgacgaagaa ggcggcgcga ttcgcgtcgc
     5221 gacgacgacc ggcctctggg gccgctggtg gctccccgcc gaagagcgcg aggagatgga
     5281 gaaccacgtc tgggtgctcg gccaatcggg caacgcgctc gagccgatcg gtcacatcgg
     5341 tggcatcgcc aagggcgagc gcatcatgac gagccgcatg cagggcaaca agggctacct
     5401 cgtcacgttc cagtacgtcg atccgctcat cacgctcgac ctcagcgaca acacgcaccc
     5461 gcgcgtcgtg ggtgagctca aggttcccgg cttctcgacg tatctccacc cgatcgccga
     5521 cggcaagctg ctctcgatcg gcatcgacaa cacggcgcac tggcgcacgc aaatctcgat
     5581 gttcgacgtc agcgacttcg cgcacccgac gctgcaagag gcgctgccgg tcgaagcgca
     5641 aaacacgtgg ggctggtcgg aagcgctcta cgatcaccac gcgttccaat actgggcgcc
     5701 gaagaagctc ctcgcgattc cgcagtcgac gtacgcctac cagaactgga acggcagcta
     5761 ctactaccag tacctctcgc agctcgtcct cgtgaacgtc gacgacaccg cgggtctctc
     5821 gatccacggc cgcatcgatc actcgagctt ctacaacgcc gacccgaaca actactggca
     5881 gtggacggac atccgtc
//
LOCUS       DATLBZ010000331         8117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_374608, whole genome shotgun sequence.
ACCESSION   DATLBZ010000331 DATLBZ010000000
VERSION     DATLBZ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374608"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VMZ53_17265"
     CDS             complement(<1..236)
                     /locus_tag="VMZ53_17265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS30263.1"
                     /translation="MKVFVIDDEREMVDLIQLGLKKRGFSVVPFNSGADALAALTEHD
                     VDVVVTDLNMKGMNGLDVCQRIETDRPDIPVIVLT"
     gene            complement(233..1675)
                     /locus_tag="VMZ53_17270"
     CDS             complement(233..1675)
                     /locus_tag="VMZ53_17270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS30264.1"
                     /translation="MRLTRKFIAALVIGVALVAMIQGFFDYRRERDVFDNQMRAEATA
                     LGRALARGMAEVWERDGEAAARQFLLTASSQSEHIRARWIWLDGTSRDAPRVSRDQLD
                     AMGDGQVVIFKESDLLLTYIPVKVPESREGGIEIAQPLGQLDKFTADSLRSGIIASAS
                     AVAIAAILALGLGFVFIGRPVGKLAEKARRVGTGDLSGPLYLRQRDELGQLAHEINVM
                     CERLAAERSARELATEQLRHADRLTTVGKLASGLAHELGTPLNVVQGRARLIRDREVE
                     GDDVIDSARIVAEQAERMTSLIRQLLDFARPRPLHKAPVNVTSLASRVCELVGTIARK
                     ANATLRAPAFDDSLRVEADDSQLQQVMTNLVINAIHAMPNGGTVDVSARIEERTPPPY
                     VGSAVTSWIAIDVRDTGLGMDAQTRERIFEPFYTTKDVGEGTGLGLSVSWGIVREHGG
                     WIDVQSEKGAGSTFTVWLPRDVARAKEAAA"
     gene            1730..2197
                     /locus_tag="VMZ53_17275"
     CDS             1730..2197
                     /locus_tag="VMZ53_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUS30265.1"
                     /translation="MLPKNILVPTDLSEGAEEALDYACELAAQFGATVHLLNVIGIPT
                     LGVPELGIALTSTMIDSMVKDNQEALEKLGQSRRSCAHIGQVLLRTGDARDTITSVAK
                     EIGADLIVMSTHGRRGVTRALLGSVTEAIVRSAPCPVLTIHPHTARHNDEKAA"
     gene            2224..2592
                     /gene="crcB"
                     /locus_tag="VMZ53_17280"
     CDS             2224..2592
                     /gene="crcB"
                     /locus_tag="VMZ53_17280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HUS30266.1"
                     /translation="MQRLLIVIAAGGIGCGVRYLVALWIGPRAFPYATLAVNLAGCFL
                     IALILESVLRGAAISPNLKLALTTGFMGGLTTYSSFNYESTSLAAEDQTSRALLYVGV
                     TLIGCVLCGLAGIWVARRLT"
     gene            2619..3296
                     /locus_tag="VMZ53_17285"
     CDS             2619..3296
                     /locus_tag="VMZ53_17285"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HUS30267.1"
                     /translation="MRWARKGGEMSTDERRERMIVDQLHRRGITDPRVLGAFEAVRRD
                     EFVDEAQARYAYDDGPLSIGYGQTISQPYIVALTVDALRLQGHERVLEIGAGSGFAAA
                     ILGKLAREVHTIDRIPELCELAATRLARLGFGNVHVHCRDGTLGLPEYAPFEAIAVAA
                     GAPSPPPSLLHQLTIGGRIVLPHGNASAQRLVRITRRAEQEFHEEDLGDVRFVPLIGA
                     EGWSAHP"
     gene            complement(3293..3835)
                     /locus_tag="VMZ53_17290"
     CDS             complement(3293..3835)
                     /locus_tag="VMZ53_17290"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HUS30268.1"
                     /translation="MSNQWDFDLSHSSVNFHVRHLMVSKVHGRFTKWNGRLELDETDL
                     TKSKLEVSIDAASVDTKEPKRDDHLRSPDFFDVEKFPSLTFVSKDITRSGDGYLVTGD
                     LTIRGITKTVRLEVEGGDRVKDPWGGTRTGFSAKTSVNRKDFGLTWNVALEAGGFVVG
                     DKIEITLEIEAIQKAATAAA"
     gene            3980..4354
                     /locus_tag="VMZ53_17295"
     CDS             3980..4354
                     /locus_tag="VMZ53_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS30269.1"
                     /translation="MERCPAVREVLHRVGDKWSVQIVALLGDGAMRFSDLRRSIEGIS
                     QRMLTLTLRGLERDGLVTRTVFPEIPPRVEYELTKLGDTLLEPIQGLADWAEKYRTSI
                     QEAREKFDAKTKKKAAAAASAA"
     gene            complement(4351..5979)
                     /locus_tag="VMZ53_17300"
     CDS             complement(4351..5979)
                     /locus_tag="VMZ53_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HUS30270.1"
                     /translation="MGEEGSQNHDFDYVVVGSGFGGSVAACRLAEKGYSVAVVEMGKR
                     WNADDFPKTTWRLRRWIWRPGLKLFGFYNMRPFKHVTILCGNAVGGGSITYANTMLVP
                     PEIVWNEGSWKGLAEWGREMPEHYATAQRMLGVTQNKILGDADRVLLKMAEDQNVGDT
                     FYKTDVAVYFGDEPGKGKPHPDPYFNGEGPPRSSCIGCGGCMVGCRYNAKNTLDKNYL
                     YFAEKRGAKIIAETRVTDVKPLGAPDGSDGYEVSTERSTAWFAKDRKTIRARHVVLAA
                     SALGTMDLLMRLKQKGSLPAVSDKLGYDVRTNSESIVGVRFPGKQYDMSKGIAIGSGI
                     YIDRFTHIEATRYSKGSDVLGTLATMLVTGSGWARIAKWIGSAFRHPIRFLRAAWPFG
                     FATQTLIFLVMQTVDATLRMRLRRPWFWPFSRVLSSVGQRIPTNIPAANTFALKAAEK
                     FGGTAITAWTEVLFDMPMTAHCIGGCVMGADPSQGVIDTQHRVFGYQNLYVVDGSAVG
                     ANLGVNPSLTITALAERAMTFIPAKAALPVKEAA"
     gene            6030..7238
                     /locus_tag="VMZ53_17305"
     CDS             6030..7238
                     /locus_tag="VMZ53_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS30271.1"
                     /translation="MAGKLKGRKAYVMAAGAVGLAGALTAASFIAPNRASAATQTYVP
                     VDAATVVAHVPQRDPKEIAERQALAAAPDRVELAVDLAREEIQRARTLSDPRYLGRAQ
                     ALLARWWSLPSPPPDVLLLRATIEQSLHQFDAARADLDALLAIRPDDAQAHLTRAVVA
                     TVRADYPAARESCRALQTLASPLVAATCEAPLDALAGDVNGAYTRLAALIDARSPASL
                     RTWALTTLAELAIQKGDNALAASHLERVLALDPDDAYARAALADALLPTDPAAASHLL
                     AGYEQVDTLLVRRAIAESRAHGPDAAKLAQQMRDRFAAAAQRQDRVHLREEAMFVLYV
                     ESNAPRALEIARDNWFVQKELADARLLAEAAVEARDAAAAQPVIEWARATGVRDARLD
                     ALLTRLGGPP"
     gene            7525..>8117
                     /locus_tag="VMZ53_17310"
     CDS             7525..>8117
                     /locus_tag="VMZ53_17310"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HUS30272.1"
                     /translation="MRRIAFLVVFLLSSTAAAHKPSDAHLKLDVRGESVTGRLDVAVR
                     DLDAAIGIDDGDGAITYGELEAAAPRIGDYVAQRLTIDGCTLSQGAPALVDLSDGAYW
                     AIPITASCEQTTALTIRYQLLFDIDAQHRGLVHVAGQTLIARDGSPVTAAMGESTSVR
                     AFVREGIWHIWEGIDHILFLLCLLLPAVWRRESAKEVA"
BASE COUNT         1344 a         2751 c         2602 g         1420 t
ORIGIN      
        1 gtgagcacga tgacgggaat gtccgggcga tcggtctcga tgcgctggca gacgtcgagg
       61 ccgttcatgc ccttcatgtt gagatcggtg acgacgacgt cgacatcgtg ctcggtgagc
      121 gcggcgagtg cgtcggcgcc cgagttgaac ggcaccacgg agaagccacg cttcttgaga
      181 ccgagctgga tgaggtcgac catctcgcgc tcgtcgtcga tgacgaagac cttcacgcgg
      241 ccgcctcctt ggcgcgcgcc acatcgcggg gcaaccagac ggtgaacgtg ctgccggcgc
      301 ctttctcgga ctgcacgtcg atccagccgc cgtgctcgcg cacgatgccc cagctgacgg
      361 agagaccgag gcccgtgcct tcgccgacgt ccttcgtcgt gtagaacggc tcgaagatgc
      421 gctcgcgggt ttgcgcatcc ataccgaggc ccgtgtcgcg cacgtcgatc gcgatccacg
      481 acgtgaccgc gctacctacg tacggcggtg gcgttcgctc ttcgatgcgc gcgctgacgt
      541 cgacggtgcc gccgttcggc atcgcatgaa tcgcgttgat cacgaggttc gtcatgacct
      601 gctgcagctg gctgtcgtcc gcctcgactc gaaggctgtc atcgaaggcc ggcgctcgca
      661 atgtcgcgtt cgccttgcgg gcaattgtcc cgacgagctc gcacacgcgc gacgcgaggc
      721 ttgtcacgtt gaccggcgcc ttgtgcaacg ggcgtggccg cgcgaagtcg aggagctgcc
      781 ggatcaggga ggtcatgcgc tcggcttgtt ctgcgacaat ccgcgccgag tcgatcacgt
      841 cgtcgccttc gacctcgcga tcgcgaatga gtcgcgccct accctgcacc acgttgagcg
      901 gcgtgccgag ctcgtgcgcg agaccgctgg cgagcttgcc gacggtggtg aggcgatcgg
      961 cgtgacgcag ttgttcggtc gcgagctcgc gggcgctgcg ttcggcggcg aggcgttcgc
     1021 acatgacgtt gatctcgtgc gcgagctggc cgagctcgtc gcgctggcgc agatacagcg
     1081 gtcccgagag atcgccggtc ccgacgcgcc gcgctttctc ggcgagcttg ccaacagggc
     1141 ggccgatgaa tacgaagccg agcccgagcg cgaggatcgc tgcgatcgcg accgcgcttg
     1201 ccgacgcgat gatgccgctg cgcagcgagt cggcggtaaa cttgtcgagc tggccgagcg
     1261 gctgcgcgat ctcgatgccg ccctcgcgcg actcggggac cttcaccggg atgtacgtca
     1321 gcaggaggtc gctctctttg aagatgacga cctgcccatc gcccatcgcg tcgagttgat
     1381 cgcgtgacac gcgcggtgca tcgcgcgagg ttccgtcgag ccagatccag cgcgctcgga
     1441 tgtgctcgct ctgactcgac gcggtcagca agaactgacg agcggccgct tcgccgtcac
     1501 gctcccagac ttctgccatg ccgcgcgcca acgcgcgtcc gagcgcggtt gcctcggcac
     1561 gcatctggtt gtcgaacacg tcgcgctcgc gccggtaatc gaagaaaccc tgaatcatcg
     1621 cgaccagggc aacaccgatt actagtgccg cgatgaactt acgggtgagc ctcatgcccc
     1681 gaggggaagt tgtagcattg gcctggttcg tgaaagctct ctaagagcga tgctgcccaa
     1741 gaacatcctc gttcccaccg atttgagcga gggagccgaa gaggcactcg actacgcatg
     1801 cgagctcgcg gcgcagttcg gcgcgacggt tcacctgctc aacgtcatcg gcattcccac
     1861 gctcggcgtt cccgagctcg gcatcgcgct gaccagcacg atgatcgact ccatggtgaa
     1921 ggacaaccag gaggcgctcg agaagctcgg ccagagccgg cgaagctgcg ctcacatcgg
     1981 ccaggttctg ctgcgcaccg gcgacgcgcg cgacacgatc acgtcggtcg caaaggagat
     2041 cggtgccgac ctcatcgtga tgagcaccca cggccgtcgc ggcgttacgc gggcgctgct
     2101 gggaagcgtc accgaggcga tcgttcgtag tgcgccatgc ccggttctga cgattcatcc
     2161 gcatacggcc cgtcacaacg acgaaaaagc cgcgtaatcg cagcgtcgta gtaacgtcgg
     2221 cgcgtgcagc gcctcttgat cgtgatcgcc gcgggtggca tcggctgcgg cgttcgctat
     2281 ctcgtcgcac tctggatcgg gccgcgggca tttccgtacg cgacgctggc cgtgaacctc
     2341 gcgggctgct tcctcattgc gctgatcctc gagtcggtac tgcgcggcgc agccatttcg
     2401 ccaaatctga agctcgcgct gacgaccggc ttcatgggcg gcctgacgac gtactcgagc
     2461 ttcaactacg agtcgacgtc gcttgcggcg gaagaccaga catcgcgtgc gctcttgtat
     2521 gtcggcgtca ctctcatcgg ctgcgtcctc tgtggacttg ccggcatttg ggttgctcgc
     2581 cgtttgacgt agacgtgcgc cgcgggcacg gggtacgcat gcgttgggct aggaagggcg
     2641 gcgagatgag caccgacgaa cgacgcgaac gcatgatcgt cgatcaactg catcgacgag
     2701 gcattactga tccgcgcgtg ctcggagcgt tcgaggcagt gcgccgagac gagttcgtcg
     2761 acgaggcgca ggcccgctac gcgtatgacg atggcccgct gtctatcgga tatgggcaga
     2821 ccatctccca gccgtatatc gtcgcgttga cggtcgatgc actgcggctg caaggtcacg
     2881 agcgcgtcct cgagatcgga gcgggctccg gtttcgcggc ggcgatcctc ggcaagctcg
     2941 cgcgcgaggt ccacacgatc gatcgcatcc cggagctctg cgagctggca gccacgcgcc
     3001 tcgctcggct tggctttggc aacgtgcacg tgcattgccg cgacggcacg ctcggcctgc
     3061 ccgaatacgc gcctttcgaa gcgatcgcag ttgcggcggg tgcaccgagc ccaccgccga
     3121 gccttctgca tcagctgacg atcggcggcc gcatcgtgtt gccgcacggc aacgcttccg
     3181 cccaacgcct ggttcgcatc acgcgacgtg ccgagcagga attccacgag gaagacctcg
     3241 gcgacgtgcg gttcgtcccg ctgatcggcg ccgagggctg gtccgcgcat ccttaggccg
     3301 ccgcagtggc cgccttctga atcgcttcga tctcgagcgt gatctcgatc ttgtcgccga
     3361 ccacaaagcc gccggcctcg agcgccacgt tccaggtgag accgaagtcc ttgcgattga
     3421 cggacgtctt cgccgagaaa cccgtgcgcg taccgcccca cggatccttc acgcgatcgc
     3481 cgccttcgac ttcgagtcgc actgtcttcg tgatgccgcg aatcgtcagg tcgcccgtaa
     3541 cgaggtagcc gtcgccgctg cgcgtgatgt ctttggacac gaacgtgagc gacgggaatt
     3601 tctcgacgtc gaagaaatct ggcgagcgca ggtggtcgtc gcgcttcggc tccttggtat
     3661 cgaccgacgc tgcatcgatc gagacttcga gcttggactt cgtcaggtcg gtctcgtcga
     3721 gctcgagcct gccgttccac ttcgtgaagc gaccgtggac cttcgagacc atgaggtggc
     3781 ggacgtggaa gttaacgctc gagtgcgata ggtcgaaatc ccattggttg gacatggcag
     3841 actccctagt aggtacttgg ttctggcttc tgccttggtt ccggcagttg ccttggttct
     3901 gtttcgttac caaggtagag ataggaacct tatgaacacc ccgcaagaag gcacacccat
     3961 gtcaccgagg aacaatcgag tggaacgctg ccccgcggtg cgcgaggtac tgcatcgcgt
     4021 cggcgataaa tggagcgttc agatcgtcgc gctgctcggc gatggagcga tgcgattcag
     4081 cgacttgcgc cggtcgatcg aaggaatctc gcagcgcatg ctcacgctca cgctgcgcgg
     4141 cctcgagcgc gacggtctcg tgacgcgaac agtctttccc gagatcccac cgcgcgtgga
     4201 gtacgagctg acgaagctcg gcgacacgtt gctcgagccg attcaaggtc tcgccgattg
     4261 ggccgagaag taccggacgt cgatccaaga agcgcgcgag aagttcgacg cgaagacgaa
     4321 gaagaaggca gccgctgccg ccagcgcagc ctaagcagct tccttcacgg ggagcgcggc
     4381 cttcgcggga atgaacgtca tcgcgcgttc cgcaagcgcg gtgatcgtga gcgatggatt
     4441 gacgccgagg ttcgcaccga cggcagagcc atcgacgacg tagaggttct ggtagccgaa
     4501 cactcgatgc tgcgtgtcga tcacgccttg cgacgggtca gcgcccatca cgcagccgcc
     4561 gatgcagtgt gcggtcatcg gcatgtcgaa gagcacttcg gtccacgccg tgatcgcagt
     4621 gccgccgaac ttctcggccg ccttgagcgc gaacgtgttt gccgcgggga tgttcgtcgg
     4681 aatgcgttgg ccgaccgacg acagtacgcg cgagaacggc cagaaccacg gccggcgaag
     4741 ccgcatgcgc agcgtcgcgt cgacggtctg catcacgagg aagatcagcg tctgcgtcgc
     4801 gaagccaaac ggccacgccg cacgcagaaa gcggatcgga tgccgaaacg ccgagccgat
     4861 ccacttggcg atccgagccc agccgctgcc ggtcacgagc atcgtggcga gcgtgccgag
     4921 cacgtccgag cccttggagt agcgagtcgc ctcgatgtgc gtgaaccgat cgatgtagat
     4981 gccggagccg atcgcgatgc ccttcgacat gtcgtactgc ttgcccggaa accgcacgcc
     5041 aacgatcgat tcggagttcg tccgcacgtc gtagccgagc ttgtccgaga ccgctggcag
     5101 cgagcccttc tgcttgagcc gcatcaatag atccatcgtg ccgagcgcgc ttgctgcgag
     5161 cacgacgtgc cgcgcgcgga tcgtcttgcg gtccttcgcg aaccacgcgg tcgaccgctc
     5221 cgtcgagact tcgtagccat cgcttccgtc gggcgcgccg agcggcttca cgtccgtcac
     5281 tcgcgtctcg gcgatgatct tcgcgccgcg cttctcggcg aagtagaggt agttcttgtc
     5341 gagcgtgttc ttcgcgttgt agcggcagcc gaccatgcag ccgccacagc caatgcagct
     5401 cgatcgcgga gggccttcac cgttgaagta cggatcggga tgcggcttgc ccttgcccgg
     5461 ctcgtcgccg aagtacaccg cgacgtccgt cttgtagaac gtgtcgccga cgttctggtc
     5521 ctcggccatc ttcagcagca cgcggtccgc gtcgccgagg atcttgttct gcgtgacgcc
     5581 gagcatgcgc tgcgcggtcg cgtagtgctc gggcatttcg cgcccccact ccgcgagccc
     5641 cttccacgag ccttcgttcc acacgatctc gggaggcacg agcatcgtgt tcgcgtaggt
     5701 gatcgagccg ccgccgaccg cgttgccgca caagatcgtc acgtgcttga acggccgcat
     5761 gttgtagaag ccgaacagct tgaggcccgg ccgccagatc cagcgccgga gtcgccacgt
     5821 cgtcttcggg aagtcgtccg cgttccaccg cttgcccatc tcgacgacgg cgaccgagta
     5881 gcccttctcc gcgagccggc acgccgcgac agatccgccg aaaccggagc cgacaacgac
     5941 atagtcgaag tcgtggtttt ggctgccctc ttcgcccatg cccgcccact atacgatccg
     6001 gatggcgccg ccgctcgtag ttgtaggcga tggcggggaa gctgaaaggc cgcaaggcct
     6061 acgtgatggc ggcaggggcc gtcggcctcg cgggcgccct gaccgctgca tcgttcatcg
     6121 caccaaatcg ggcgtccgcc gcgacgcaga cctacgtgcc cgtcgacgcg gcaactgtcg
     6181 tcgcgcacgt gcctcagcgc gatcccaaag agatcgccga gcgccaggca ctcgcggcgg
     6241 cgcccgatcg cgtcgagctc gctgtcgatc tcgcacgcga agagatccag cgcgcccgca
     6301 cgctctcgga ccctcgctac ctcgggcgcg cgcaagcgct gctggctcgc tggtggtccc
     6361 tcccctcgcc gccacccgac gttctcctgc tccgcgcaac gatcgaacaa tcgctccacc
     6421 agttcgacgc tgcgcgcgca gacctcgacg cgctactcgc gatccgcccc gacgacgcgc
     6481 aagcgcacct cacacgcgcc gtcgtcgcca cggtccgtgc cgactacccc gcagctcgcg
     6541 agagctgtcg cgcgctgcag acgctcgcgt cgccgctcgt cgccgcgacg tgcgaagcgc
     6601 cgctcgacgc gctcgccggc gacgtcaacg gcgcctacac ccgcctcgcc gcactgatcg
     6661 acgcgcgctc tccggcctca ctccgcacgt gggcgctgac aacgctcgcc gagctcgcga
     6721 ttcaaaaggg cgacaacgcg ctcgccgcct cgcacctcga acgcgtgctc gcactcgatc
     6781 ccgacgacgc atacgcccgc gcagcgctcg ccgacgcgct cctgccaacc gacccggccg
     6841 ccgcctctca cctgctcgcc ggctacgagc aagtcgacac gctgctcgtc cgccgcgcga
     6901 tcgccgaatc ccgcgcgcac ggccccgacg ccgccaagct cgcgcagcaa atgcgcgacc
     6961 gcttcgccgc cgcagcacag cgccaagatc gcgtgcacct ccgcgaagaa gcgatgttcg
     7021 tgctctacgt cgagagcaac gcgccacgcg cgctcgagat cgctcgcgac aactggttcg
     7081 tgcagaagga gctcgccgac gcacgcctgc tcgccgaggc tgcggtcgaa gcgcgcgatg
     7141 cagccgccgc gcagccggtc atcgaatggg cgcgcgccac cggtgtccgc gatgcgcgcc
     7201 tcgacgcgct gctcacgcgc ctcggaggtc cgccatgaag ctccacgtcg acgtcgtcga
     7261 tccgctaccg ccgctcgcag ctccccgctc ggacgaccgt gcactgtcga tccgtcgctg
     7321 gctcgtcctg gcatccgccc ggaccccaga tggaacacaa aggcgcaaag acggcaaagg
     7381 cgcagaacag ggtcaaggcg cgccgtctgc tcacacgcac gctcctgcag agcgcgccct
     7441 taccccgacc tctttttccg cggcctctgc cttctcggcg tcttcgtgtt ccagcttctc
     7501 cggccaggct cgcggaggtc gtcgatgagg cgcatcgcat ttctcgtcgt gttcttgttg
     7561 tcgagcaccg cggcggcgca caagccgagc gatgcacatc tgaagctcga tgtgcgtggt
     7621 gagagcgtca cggggcggct cgacgttgcg gtccgcgatc tcgacgctgc gatcggcatc
     7681 gacgacgggg acggtgccat cacctacggc gagctcgaag ctgcggcacc gcggatcggc
     7741 gactacgtcg cgcaacgtct cacgatcgac ggctgtaccc tcagccaagg cgcacccgcg
     7801 ctggtcgatc tgtccgacgg cgcgtactgg gcgatcccga tcacggcgag ctgcgagcag
     7861 acaacggcac tgacgattcg ctatcagctg ttgttcgaca tcgacgcgca gcatcgcggg
     7921 cttgtccatg tcgcggggca gacactcatc gcgcgcgatg gatcgcccgt gaccgcggcg
     7981 atgggcgagt cgacgtcggt gcgcgcgttt gtccgcgagg gcatctggca catctgggag
     8041 ggcatcgacc acattctgtt tctgctgtgt ctgctgctgc ctgcggtgtg gcggcgcgag
     8101 tccgcgaagg aagtcgc
//
LOCUS       DATLBZ010000332         3414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4085427, whole genome shotgun sequence.
ACCESSION   DATLBZ010000332 DATLBZ010000000
VERSION     DATLBZ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4085427"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..777
                     /locus_tag="VMZ53_17315"
     CDS             46..777
                     /locus_tag="VMZ53_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30273.1"
                     /translation="MQMHGLTWNTDLAAAITKARAFKRPILSLRLLGRLDEELTCANS
                     RFFKKLLYPNPTINQLLRDHFILHWQSVRPVPIVTIDFGDGRRIRKTLTGNSVHLVLD
                     PDGRPADALPGLFSPHVFSALLMRAHGYALADRSKLPELHRQALAQPVPPVTESRAMR
                     ASMIAPTKHMVEMPMLRAVSPLSDIEGDTRTNLALHARIHQAFASGAQWSSVDAMVAR
                     IYEDLFEMPIDDPALGLDVPDPFAA"
     gene            complement(774..1160)
                     /locus_tag="VMZ53_17320"
     CDS             complement(774..1160)
                     /locus_tag="VMZ53_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS30274.1"
                     /translation="MLHIKLTSVMVEDQAKALAFYTDLLGFEKTMDFPVGEYRWITVA
                     AKGRDDLQLALEPNANPAGKAFQQAMFAQSIPVASLESDDLDADYARLTKAGVAFTMP
                     PTTAGAVKIAVFSDTVGNLIQIHQTL"
     gene            complement(1179..1592)
                     /locus_tag="VMZ53_17325"
     CDS             complement(1179..1592)
                     /locus_tag="VMZ53_17325"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HUS30275.1"
                     /translation="MLIDPRTFRRLVTVRDQLVETDVALYELAADAGLSPSRLIRVFA
                     AVFGTTPHRARTQARLERARTHLASGAAVTDACMEVGFSSVGSFSALFTRWTGTQPSR
                     YRRMVQVAATVAPLVPGCLGMLAALPRNFREARFA"
     gene            complement(1596..2294)
                     /locus_tag="VMZ53_17330"
     CDS             complement(1596..2294)
                     /locus_tag="VMZ53_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30276.1"
                     /translation="MRSVALLLVLIACRSNEDESVKKLEAKQNDLDRRVGAVEQRIPV
                     DTIAVANELLAKGSAAGLHGPAGAQGPEGPAGPAGPMGPAGVGPPGPAGERGPKGDPG
                     PAGPEGPQGIQGLQGAQGIQGQQGAQGPKGPPGPPGAYGHKSDLVRKENRISVGAGLV
                     ATAVAPCDRAQDLLVVGGCYADPQWMAQLVASRPVAMTDQANPAAWRCDYKNISPSST
                     IEVVAEAYCVAPRE"
     gene            2353..3165
                     /locus_tag="VMZ53_17335"
     CDS             2353..3165
                     /locus_tag="VMZ53_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS30277.1"
                     /translation="MIEMVDVRLDHPRGGEPLVSEANLKVARGEVVMIVGAAGVGTSR
                     MVAATLGELPASSGRIEVMGRDVSKLRRASLRLMRRRVGVVPQDLCLLDDRSAQLNVC
                     MPLEIDGIPRSVSILKAGEVLDAMKLSSEASLPVECLSAAEKQRVAVARALVRSPDIV
                     VADHPTSMQDAAGAELVCNALEAAAKRGAAVIVLGRDPALRAMAEKLGWTMYVLVEGK
                     LRAMAELGEAVEEVLASVESVQVAPLEEVAPPAEDEDNVVPFPLSARTAGVA"
     gene            3195..3362
                     /locus_tag="VMZ53_17340"
     CDS             3195..3362
                     /locus_tag="VMZ53_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30278.1"
                     /translation="MAPRHVTRGRKEFDVTDATMEPDPGPAPEAIFDRMTGPWDRRRS
                     ISAPGAADEET"
BASE COUNT          573 a         1097 c         1140 g          604 t
ORIGIN      
        1 gcgactctta catttcgcta acacaccagg tcctagcttg ggaccatgca gatgcacggt
       61 ctcacctgga acaccgatct cgctgccgcc atcaccaagg cccgcgcctt caagcgcccg
      121 atcttgtcgc tgcgtcttct cggccgcctc gacgaggagc tgacgtgcgc gaatagtcgc
      181 ttcttcaaga agctgctgta tcccaatccg acgatcaacc agctgctgcg cgatcacttc
      241 atcttgcatt ggcagtcggt ccgtcccgtt ccgatcgtta cgatcgattt tggcgacggt
      301 cgccgcattc gcaagacgct caccggcaac agcgtgcacc tcgtgctcga tcccgacggt
      361 cgtcctgcag acgcgctgcc cggcctcttc tcgccgcacg tgttcagcgc actcttgatg
      421 cgcgcgcacg gatacgcgct cgccgaccgc agcaagctcc ccgagctgca ccgccaggcg
      481 ctcgcgcagc cggtgccgcc cgtgacggag tcgcgtgcga tgcgcgcgag catgatcgcg
      541 ccgacgaagc acatggtcga gatgccgatg ctacgcgccg tgtcaccgct ctctgatatc
      601 gaaggagaca cgcgcacgaa tctcgcgctg cacgcgcgca ttcaccaggc gtttgcttcg
      661 ggcgcgcagt ggtcgagcgt cgacgcgatg gtcgcgcgca tctacgaaga cctgttcgag
      721 atgccgatcg atgatccggc gctcggcctc gacgtccccg atccgttcgc ggcttagagc
      781 gtctgatgga tctggatcag attgccgacg gtgtccgaga agaccgcgat cttcaccgcg
      841 cctgcagttg tcggcggcat cgtgaacgcg acgcctgcct tcgtgagccg cgcgtaatcg
      901 gcgtcgagat cgtcggactc gagcgacgcg accgggatcg actgcgcgaa catcgcctgc
      961 tgaaatgctt tgccggcggg attcgcgttc ggctcgagcg cgagctggag atcatcgcgg
     1021 cccttcgctg cgaccgtgat ccagcggtat tcgccgaccg gaaagtccat cgtcttctcg
     1081 aagccgagga ggtctgtgta gaaggcgagg gccttggctt ggtcctcgac catgacgctg
     1141 gtgagcttga tgtgaagcat gtgccgagac tggcgagctt acgcgaagcg cgcttctcga
     1201 aaattgcgcg gcagcgcggc aagcatgccg agacagccgg gcactagcgg cgcgacggtc
     1261 gcagcgactt ggaccatgcg gcgatagcgg ctcggctgcg tgcccgtcca gcgcgtgaac
     1321 agcgccgaga agctgccgac gctcgaaaac ccgacttcca tgcaggcgtc ggtgaccgcg
     1381 gcgcccgacg cgaggtgcgt gcgcgcgcgc tccagccgcg cttgcgtgcg agctcgatgt
     1441 ggcgtcgtgc cgaacaccgc ggcgaacacg cgaatcaggc gcgacggcga gagacctgcg
     1501 tccgcggcta gctcgtagag cgcgacgtca gtctcgacga gctggtcacg aaccgtgacg
     1561 agacgacgaa acgttcgtgg atcgatcagc acgggtcact cgcgtggtgc cacacaatag
     1621 gcttcggcga caacctcgat cgtcgacgac ggcgagatgt tcttgtaatc gcagcgccac
     1681 gcggcggggt tcgcttgatc ggtcatcgcg acggggcgcg acgcgacgag ctgcgccatc
     1741 cattgcggat cggcgtagca accaccgacg acgagcaggt cctgcgcacg atcgcacggc
     1801 gcgacggcgg tggcgacgag gcccgccccg acggagatgc gattctcttt gcgcacgaga
     1861 tccgatttgt gtccgtacgc accgggagga cccggcgggc ctttcggccc ctgcgcgccc
     1921 tgctggcctt gaatgccttg cgcgccttgg agaccttgga tgccttgcgg tccttccgga
     1981 ccggctggac cgggatcgcc tttggggcct cgctcgcccg caggacccgg cggaccgacg
     2041 ccggctggac ccattggccc ggctggaccc gcgggacctt ccgggccttg tgcaccggca
     2101 gggccatgca gaccagcggc gctgcccttc gcgagcagct cgtttgcgac ggcaatcgta
     2161 tcgaccggga tgcgttgctc gacggcacct acacggcgat cgagatcgtt ttgcttcgct
     2221 tccagttttt ttacgctctc gtcttcgttt gatcgacaag cgatcagaac gagcaggaga
     2281 gccaccgagc gcatgggtga atcctacgac gtttttttgc cctcaccgat cagatgggcg
     2341 tagctggtgc acatgatcga gatggtcgac gtccgactcg accacccacg cggtggcgag
     2401 ccgctggtga gcgaggcaaa tctcaaggtc gcacgtggcg aggtcgtcat gatcgtcggt
     2461 gcggcgggcg tcggaacgtc gcgcatggtc gcggcgacac tcggcgagct cccagcatcg
     2521 agtggccgca tcgaagtgat gggccgcgat gtgagcaagc tgcggcgcgc gtcgctgcgg
     2581 ctgatgcggc ggcgggttgg cgtcgtgcca caggacttgt gcctgctcga cgatcgcagc
     2641 gcgcagctca acgtctgcat gccgctcgag atcgacggca tcccgcgctc ggtatcgatc
     2701 ttgaaagcag gcgaggtgct cgatgcgatg aagctctcgt cggaggcatc gctgcccgtc
     2761 gagtgcttgt ccgcggcgga gaagcagcgc gtggcggttg cgcgtgcgct cgtgcggtcg
     2821 ccggatatcg tcgtcgccga tcacccgacg agcatgcaag acgcggccgg cgcagagctc
     2881 gtgtgcaatg cgctcgaagc tgcggcgaag cgcggcgcag ccgtgatcgt gctcggtcgc
     2941 gatccagcgc tgcgcgcgat ggcggagaag ctcggctgga cgatgtacgt cctcgtcgag
     3001 ggcaagctgc gtgcgatggc cgagctcggc gaagcggtcg aggaagtact ggcgagcgtc
     3061 gagagcgtgc aggtcgctcc gctcgaggaa gtggctccgc ccgccgaaga cgaagacaac
     3121 gttgttccct ttccactcag cgcacgaacg gcaggtgttg cgtgaagatg cgaacgctcc
     3181 gcaagctacg aacgatggca ccgcggcacg tcacgcgcgg gcgtaaagag ttcgatgtca
     3241 ccgatgcgac gatggagccc gatccgggcc ctgcgcctga agcaatcttc gatcgcatga
     3301 cggggccgtg ggatcgacgc cgttcgatct cggctcccgg cgctgccgac gaggagacct
     3361 aaggctctgt gccgtcgtga tcaccgcacg tatcgctagt gcgttgcgtc ggac
//
LOCUS       DATLBZ010000333         5410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9319462, whole genome shotgun sequence.
ACCESSION   DATLBZ010000333 DATLBZ010000000
VERSION     DATLBZ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9319462"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VMZ53_17345"
     CDS             <1..234
                     /locus_tag="VMZ53_17345"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HUS30279.1"
                     /translation="GFQGQATDIEIHTKEILKTRDTLNDLYSKHTGQPTDKIKVDTDR
                     DNFMSAPQAKEYGIIDEVLITAKKKPDEKAEKK"
     gene            258..1526
                     /gene="clpX"
                     /locus_tag="VMZ53_17350"
     CDS             258..1526
                     /gene="clpX"
                     /locus_tag="VMZ53_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HUS30280.1"
                     /translation="MPPIEKRDGASLTCSFCGKAQKEVKKLIAGPTVYICDECIGLCN
                     DIIAEEIEKEDQSYGTSSIPKPAQIKKVLDEYVIGQDRAKKILAVAVHNHYKRIDHKQ
                     AGGAPDEEVELQKSNILLLGPTGSGKTLLAQTLARILNVPFAIADATNLTEAGYVGED
                     VENIIVSLLQNADHDIERAQRGIVYIDEIDKIARKSDNPSITRDVSGEGVQQALLKII
                     EGTTASVPPKGGRKHPQQEFLQVDTTNILFICGGAFTGIEDIIENRVGQKLIGFGAGM
                     NKKIDRTQWELLQEIEPEDLLKYGMIPEFIGRLPVIAPLHELSEEALVQILTQPKNAL
                     VKQYQKLFEMDGVKLKFGKGALTKVANMAASQKSGARGLRAILESALLDIMYDTPGQS
                     VSEVIINEDVIEKHGSPLITYAKESKAAEA"
     gene            1600..2763
                     /locus_tag="VMZ53_17355"
     CDS             1600..2763
                     /locus_tag="VMZ53_17355"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUS30281.1"
                     /translation="MRRWSEGRPRTFAGLNVDRGQVVASELDPELQQLTERVSRELGV
                     PRAAVVLGDDDLAVLRAHYGFPDYEHVTARTSSFCKFVVRDGVYFEVNDARHDERLPQ
                     DAVESLGISSYLGAPIVLQGKTVGTMCVVDTEPRLFDDRERGILHRFAALASRRLSLL
                     ATQPRELERTLHDRAVRPAFSEIRNRLQPVLGNVATMQGLMQQLVAARIELDTTTDST
                     RVAEIRAKADAVIADLVTCLEDTTTDTNILHRSITAIERASLVSQDMCSIHDVIGPAT
                     TLAYHRTKLVRGVSWSGESREMLSTARGVAVTALAAALAGVAESIERGATHGITASIS
                     STDDVVLIELRAEVSPPALAELASHLAVLVGDSSHVRVHDRALQLRFATLQAS"
     gene            complement(2677..4086)
                     /locus_tag="VMZ53_17360"
     CDS             complement(2677..4086)
                     /locus_tag="VMZ53_17360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS30282.1"
                     /translation="MRFRTQLVSGIGALVLVTIFCGAMAVITLRVTTKSAERVTSRVV
                     DDLEALHDVRLDAEQLVAVGRGYLLTGDDADHRHFTELQKELDTSLEALRGRERAELA
                     TKIAAVEVAARDYAKIVAQAVETRPPGGSLATTTQIVENNIAPKRATLQAAVDDLTDL
                     EQSDLTNVSMHSGRVVQAVGITTLIGCLIGVAIGIALAIVITRRLTGEYRRQEQEART
                     ATAAAWSRKELLDIVSHDLRSPLNAIMLGLEIVKDKHADDRTVAVISHSAERMQRLVN
                     DLLDASRAELAQLELELHPTQPNTLIDVARDQFEGLARAAGVTLETSCSADGTLVCDR
                     ERILQILSNLVGNAIKFTKAGDTVAVAASSSDAGVRFAVSDHGPGIPPDEQARMFEAY
                     KQGSTTSHRGGIGLGLYICKKLVEAHGGRIGVTSTEGQGSTFWFELPRQLARLQRRES
                     QLQRTVVNPHMRAIADEHG"
     gene            4207..>5410
                     /locus_tag="VMZ53_17365"
     CDS             4207..>5410
                     /locus_tag="VMZ53_17365"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HUS30283.1"
                     /translation="MVDDSDWYKKLSFAVAASLALGANACGSTVDPGEDAPPDEQGPI
                     LQHASRSSTIALSDDGALVAMVNPDDGSLSVFQTSDNTRLARVQTGGSPSSVVIAADN
                     TTAYVANRADGTVVRVAGLDTAAAAVDATIDVGAEPMGLALSPTGKQLFVAEFAESRV
                     SIVDTKTNTVANSFPFDRPRALMVTNNLDSSDDDEQLIVAEFFGRPVAEAKDDGRTGY
                     LRMVSLADITQTKGITLAAAFDSGFPKLGVATNPTVKTSPNQLGALNIAAGRLYVTSV
                     SASPEGPPRFDNNVYPVVYVADLASAAEVRDASGTVNLARKIYDAIPSPSATNPRFLP
                     GDLTDIDFVPGSTVGYAIGRAGDVMVRVTFASDVQIGSTQNKLIDLAGNDTIGKCQGP
                     TGLIVDKNH"
BASE COUNT         1032 a         1744 c         1732 g          902 t
ORIGIN      
        1 ggcttccaag gtcaggcgac ggacatcgag atccacacca aagagatctt gaagacgcgt
       61 gacacgctga acgacctgta cagcaagcac acgggtcaac cgacggacaa gatcaaggtc
      121 gacaccgacc gcgacaactt catgtcggcg ccgcaggcca aggaatacgg catcatcgat
      181 gaggtgctga tcacggccaa gaagaagccc gacgagaagg ccgagaagaa gtagctctgc
      241 tacgctaacc gtcgaagatg cccccgatcg agaagcgtga tggagcctcg ctgacctgct
      301 ccttctgcgg caaggcgcag aaggaagtga agaagctgat cgcaggtccg acggtttaca
      361 tctgcgacga gtgcatcggc ctctgtaacg acatcatcgc cgaggagatc gagaaagagg
      421 accagtcgta tgggacctcc tcgatcccga agccggcgca gatcaagaaa gtcctcgacg
      481 agtacgtgat cgggcaggac cgcgcgaaga agatcctcgc ggtcgcggtc cacaatcact
      541 acaagcgcat cgaccacaag caggccggtg gagcgccgga cgaagaagtc gagctgcaga
      601 agtcgaacat cctcctgctc ggtcccaccg gctcgggcaa gacgctgctc gcacagacgc
      661 tcgcgcgcat tctcaacgtt ccgttcgcga tcgcggacgc gacgaacctc accgaggccg
      721 gttacgtcgg cgaggacgtc gagaacatca tcgtctcgct gctccagaac gccgatcacg
      781 acatcgagcg tgcgcagcgc ggcatcgtct acatcgacga gatcgacaag atcgcccgca
      841 agagcgacaa cccgtcgatc acgcgcgatg tgtccggtga aggcgtgcag caggccctgc
      901 tcaagatcat cgagggcacg accgcgagcg ttccgccgaa aggtggtcgg aagcatccgc
      961 agcaagagtt cctgcaggtc gacacgacga acatcctgtt catctgcggc ggtgccttca
     1021 ccggcatcga ggacatcatc gagaatcgcg tcggtcagaa gctcatcggc tttggcgccg
     1081 gcatgaacaa gaagatcgat cgcacgcagt gggagctgct gcaggagatc gagcccgagg
     1141 atcttctcaa gtacggcatg attcccgagt tcatcggtcg tctgcccgtg atcgcaccgc
     1201 tgcacgagct gtcggaagag gcgctcgttc agatcctcac gcagccgaag aacgcgctcg
     1261 tgaagcagta ccagaagctg ttcgagatgg acggcgtgaa gctcaagttc ggcaagggcg
     1321 cgctgacgaa ggtcgccaat atggcggcgt cgcagaagag cggtgcccgc ggccttcgcg
     1381 cgatcctcga gagcgcgctg ctcgacatca tgtacgacac gccgggccag tcggtctcgg
     1441 aagtgatcat caacgaggac gtgatcgaga agcacggctc gccactgatc acctacgcga
     1501 aagaatccaa ggccgccgag gcctgacccg ctaacccacc gcaaccccag ctgtttgttt
     1561 ggggtgaaat ttcagcgcgg ttggtcgatc cagtagtgca tgaggcgatg gagtgagggg
     1621 cggccgagga cgttcgcagg actcaacgtc gatcgcggcc aggtggtggc gagcgagctc
     1681 gatccagagc tgcagcagct caccgaacgc gtcagtcgtg agctcggcgt gccgcgtgcg
     1741 gcggtcgtgc tcggggacga tgacctcgcc gtgttgcgcg cgcactatgg gtttcccgac
     1801 tacgagcacg tgaccgcgcg aacctcgtcg ttctgcaagt tcgtcgttcg cgacggcgtg
     1861 tacttcgagg tcaacgacgc tcggcacgac gagcgcttgc cgcaggatgc ggtcgagagc
     1921 ctcggcatct cgtcgtatct cggcgcgccg atcgtgctgc agggcaagac cgtcggcacg
     1981 atgtgcgtcg tcgataccga gccacggctg ttcgacgatc gcgagcgagg catcctgcac
     2041 cgcttcgccg cgctcgcgag tcgccgcctc tcgctgctgg ccacgcaacc acgcgagctc
     2101 gaacgcacgc ttcacgatcg cgcggtgcgg ccggcgttct cggagatccg caatcgactg
     2161 cagcccgtac tcggcaacgt ggcgacgatg caagggctca tgcagcagct cgtcgcggcg
     2221 cgcatagagc tcgacaccac aaccgactcg acgcgcgtag cggagattcg ggcgaaagcc
     2281 gacgctgtca tcgccgacct cgtgacctgc ctcgaggaca cgacgacgga cacgaacatc
     2341 ttgcatcgtt cgatcaccgc gatcgagcgc gcgtcgctcg tctcgcagga catgtgctcg
     2401 atccacgatg tgatcggccc cgcgaccacg ctcgcgtatc accgcaccaa gctcgtccgc
     2461 ggcgtgagct ggagcggcga gagccgggag atgctgagca ccgcgcgtgg cgttgcggtg
     2521 actgcgcttg cggcagcact ggcaggcgtc gccgaatcga tcgagcgcgg cgctacccac
     2581 ggcatcacgg cgtcgatcag tagcaccgat gacgtcgttc tcatcgagct tcgcgccgag
     2641 gtctcaccgc ctgccctggc ggagctcgcg agccatctag ccgtgctcgt cggcgatagc
     2701 tcgcatgtgc gggttcacga ccgtgcgctg cagctgcgat tcgcgacgct gcaagcgagc
     2761 tagctgccgc ggcagctcga accagaacgt gctgccctgg ccctccgtgc tcgtcacgcc
     2821 gatgcgaccg ccatgcgctt cgacgagctt cttgcagatg tagaggccaa ggccgatacc
     2881 gccgcgatgc gacgtggtcg agccctgctt gtatgcctcg aacatccgcg cttgctcgtc
     2941 cggcggaatg cccgggccgt gatccgagac ggcgaaccgt acgcccgcat cgctcgagct
     3001 ggccgcgacc gcaacggtgt cgcccgcctt cgtgaacttg atcgcgttgc cgacgaggtt
     3061 cgagaggatc tggaggatgc gctcgcgatc gcagacgagc gtgccgtccg cgctgcatga
     3121 cgtctcgagg gtcacgccgg ccgcgcgagc gaggccctcg aactggtcgc gcgcgacgtc
     3181 gatcagtgtg ttcggctgcg tcgggtgcag ctcgagctcc agctgggcga gctcggcacg
     3241 cgatgcatcg agcagatcgt tgacgagccg ctgcatgcgc tctgcggagt gcgagatcac
     3301 cgcgacggtg cgatcgtcgg cgtgtttgtc tttgacgatc tcgaggccga gcatgatcgc
     3361 gttcagcggc gagcggagat cgtgcgagac gatgtcgagc agctctttgc gagaccacgc
     3421 ggcagccgtt gcagtccgcg cttcttgctc ttgccggcgg tactcgccgg tgagcctacg
     3481 cgtgatgacg atcgcgagcg cgatgccgat cgcgacgccg atgaggcacc cgatcagcgt
     3541 cgtgatgccg accgcctgca cgacgcgacc ggagtgcatc gacacgttcg tcagatcgga
     3601 ttgctcgagg tcggtgaggt catcgaccgc ggcctggagc gtcgcgcgtt taggggcgat
     3661 gttgttttcg acgatctggg tcgtggttgc gagcgagccg cccggaggcc tcgtctcgac
     3721 agcctgcgcg acgatcttcg cgtaatcgcg cgccgctacc tcgacagcgg cgatcttggt
     3781 cgcgagctct gcacgctctc gcccacgcag cgcttcgagc gatgtatcga gctccttctg
     3841 tagctcggtg aagtggcgat gatccgcatc gtcgccggtg agcagatagc cgcggccgac
     3901 ggcgacaagc tgctcggcgt cgagccgcac atcgtgcagt gcttcgagat cgtcgacgac
     3961 ccgactcgtc acgcgctcgg cactcttcgt cgtgacgcgc aacgtgatga ccgccatcgc
     4021 gccgcagaag atcgtcacca gaacgagcgc accaatgccc gacacgagct gtgtgcggaa
     4081 tctcatcgtc tgcactgtgc cgcggcttcc gtcacgtgca accgaatctg ctcgcgctcc
     4141 ggacacttac ggcattttgc gacaacggcc gagagccctc ctctcaatcg caggagggga
     4201 aactcgatgg ttgatgactc cgattggtac aagaagctta gtttcgcggt tgccgcatca
     4261 ctggctctcg gcgcgaatgc gtgcggtagc accgtagacc cgggtgagga tgcaccgccg
     4321 gacgaacaag gtccgatcct gcaacacgcc tcacgctcga gcacgatcgc gctgtctgat
     4381 gacggagcgc tcgtcgcgat ggtcaacccc gacgatggca gcttgtcggt gttccagacg
     4441 agcgacaaca cgcgactcgc gcgcgtgcag acaggcggct cgccttcgag cgtcgtgatc
     4501 gcggccgaca acacgaccgc ctacgtcgcg aatcgcgcag acggcacggt cgtgcgtgtt
     4561 gcgggtctcg atacagcggc cgctgcagtc gatgcgacga tcgacgtcgg cgcggagccg
     4621 atgggtctcg cgctctcgcc gaccggcaag cagctgttcg tcgcagagtt tgcagagagc
     4681 cgagtatcga tcgtcgacac caagaccaac acggtcgcca attcattccc gttcgatcgc
     4741 ccgcgcgcgc tgatggtgac gaacaacctc gatagcagcg atgacgacga gcagctgatc
     4801 gtcgccgagt tcttcggacg gccagttgcc gaggcgaagg acgatggccg cactggctac
     4861 ctgcgcatgg tgtcgctcgc cgacatcacg cagacgaagg gcatcacgct cgccgccgcg
     4921 ttcgacagcg gcttccccaa gctcggcgtc gcgacgaacc cgacggtcaa gacgtctccg
     4981 aaccagctcg gcgcgctgaa catcgcagcc ggccgcctct acgtgacgag cgtgtccgct
     5041 tctcccgagg gcccgcctcg cttcgacaac aacgtctatc ccgtcgtcta cgtcgccgac
     5101 ctcgcgagcg ctgcagaagt gcgcgacgcg agcggcacgg tgaacctcgc acgcaagatc
     5161 tacgatgcga tcccgagccc gtcggcgacg aacccgcgct tcttgcccgg cgatctgacg
     5221 gacatcgatt tcgtcccggg ctcgacggtc ggctacgcga tcggccgcgc gggtgacgtg
     5281 atggtccgcg tgacgtttgc ctccgacgtg cagatcggct cgacgcagaa caagctgatc
     5341 gatctcgcgg gcaacgacac gatcggcaag tgccaaggcc cgaccggcct aatcgtcgac
     5401 aagaatcaca
//
LOCUS       DATLBZ010000334         3541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10628258, whole genome shotgun sequence.
ACCESSION   DATLBZ010000334 DATLBZ010000000
VERSION     DATLBZ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10628258"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2427
                     /locus_tag="VMZ53_17370"
     CDS             <1..2427
                     /locus_tag="VMZ53_17370"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HUS30284.1"
                     /translation="YGLAGMCGYLLIALGVAAAIRGLLGRTPAMPPLVVAGTLLAVVA
                     LATLVHLIAPHYRVAGHGPGGGIGEHLAEILRAVISTAGTALLSLVGLVVAVVIATPL
                     SMREVLHTIWGGMRFLGGGLRTAAFAFAGFWGDVFRALLPERDRDDEDEDEEVEEEDV
                     LEASDDEHGPEPVIIERTQPSAIEVVELTPAEKKQRKKKEAAKTEIDFAVPGPAANDE
                     DVVEVEAAPKKGRERMAKGTVAPPPAEPTPDDHAGPVIFEPKFKAQDKATMAAKEKQA
                     EAERQTFIKLGEGEYQLPSIQLLNYDAKEQNTIDKTAMLELSAKLTQTLENYGVKGDV
                     VAIRPGPVVTMYEFAPAPGTRVNKIVNLTDDLALALEALRVRIVAPIPGKAAVGIEVP
                     NKTREKVFIKEILADDAFRKARARLPMAIGKDIEGAPFVVDMARMPHLLVAGTTGSGK
                     SVGVNSMITSLLYHASPEDVRMIMVDPKMLELSIYEGVPHLLLPVVTDPKKANLALRW
                     AVEEMERRYDLLAQQGVRDIIGYNEKVAKQQAKLEADKLREAADAAAKALEEDLEKVI
                     SADSPEAADAREKVETAQHQHKLPYIVVVIDEFADLMMCAPKEVETSVARIAQKARAA
                     GIHLILATQRPSVDVITGLIKANFPSRIAFSVASKIDSRTILDQNGAEALLGAGDMLF
                     SDRGASPVRLHGCYVDESEIHRVVDHLKTQGRPVYNLDIVKPRDEEGEGGNEFGGGGG
                     GGGKSDDDMYDKAVYIVTTTRNASISWVQRQLRIGYNRAARLVEEMEKQGVVSPPDHT
                     NKREVLVAAA"
     gene            complement(2424..2891)
                     /locus_tag="VMZ53_17375"
     CDS             complement(2424..2891)
                     /locus_tag="VMZ53_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30285.1"
                     /translation="MSRMTVVPNADQLLLAGSIDESAKLTDLIAQAKNGRLVLDLAGI
                     TFINSIGVREWCYMQQAAAKAKVAIELRRVAEPIVHQLNIVPAARGVSLVSSFYAPYE
                     CDECDREFVMLLDVRTYGNDLARMRAPALKCPDCGDALVFGHPPELYFTFLGN"
     gene            2922..>3541
                     /locus_tag="VMZ53_17380"
     CDS             2922..>3541
                     /locus_tag="VMZ53_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUS30286.1"
                     /translation="MRATWLTDVHLNFMRPLALRAFYERVRQERPDCLFVTGDIGEAD
                     SVGRFVEELGRIAPVYYVLGNHDYYRAGIHAVRENLPAGWLPAAGPVALTEQTVLLGV
                     DGWGDARCGDLDSNVQLSDWQLIEDFERVRYDRKARVALLQELGTAEAFVLGELLARA
                     PASQRLVVLTHVPPFPEACVYDGAQSEPAWLPWFTCIATGQLLAEYA"
BASE COUNT          652 a         1149 c         1173 g          567 t
ORIGIN      
        1 tacggcctcg ccgggatgtg cggctacctg ctgatcgcgc tcggcgtggc agcggcgatt
       61 cgcgggctgc tcggccgcac gcctgcgatg ccaccgctcg tggtcgcggg aacgctgctc
      121 gctgtcgtcg cgctcgcgac gctcgtgcac ctgatcgcgc cgcactaccg cgtcgccggc
      181 cacggtccgg gtggcggcat cggcgagcat ctcgcggaga tcctgcgcgc agtgatcagc
      241 acggcgggca cggcgctgct ctcgctggtc ggcctcgtcg tggcagtcgt gatcgcgacg
      301 ccgctctcga tgcgcgaagt gctccacacg atctggggcg gcatgcgctt cctcggcggt
      361 ggcctgcgca cggctgcatt cgcgttcgcc ggcttctggg gcgatgtgtt ccgcgcactc
      421 ttgcccgagc gggatcgcga tgacgaggac gaggatgaag aagtcgaaga agaggatgtc
      481 ctcgaagcga gcgacgacga gcacggtccc gagccggtga tcatcgagcg cacgcagccg
      541 tccgcgatcg aggttgtcga gctcacgcca gccgagaaga agcagcgcaa gaagaaggaa
      601 gcggcgaaga ccgagattga ttttgctgtg cccggtcctg ccgccaacga cgaggacgtc
      661 gtcgaagtgg aggctgcgcc gaagaagggt cgcgagcgca tggcgaaggg caccgtggct
      721 ccgcctcctg ccgagccgac gcccgacgat cacgcaggcc cggtgatctt cgagccgaag
      781 ttcaaggcgc aggacaaggc gacgatggcc gcgaaggaga agcaggccga ggcggagcgc
      841 cagacgttca tcaagctcgg cgagggcgag taccagctgc cgtcgatcca gctcctcaac
      901 tacgacgcca aagagcagaa cacgatcgac aagaccgcga tgctcgagct ctccgcaaag
      961 ctgacgcaga cgctcgagaa ctacggcgtg aagggcgacg tcgtcgcgat ccgcccgggt
     1021 cccgtcgtca cgatgtacga gttcgcgccg gcgcccggta cgcgcgtgaa caagatcgtc
     1081 aacctcaccg acgacctcgc actcgcgctc gaagcactgc gcgttcgcat cgtcgcgccg
     1141 attcccggca aagccgccgt cggcatcgag gtcccgaaca agacgcgcga gaaggtcttc
     1201 atcaaggaga tcctcgccga cgacgcgttc cgcaaggcgc gcgctcgtct accgatggcg
     1261 atcggcaagg acatcgaggg tgcgccgttc gtcgtcgata tggcgcgaat gcctcacctg
     1321 ctcgtcgctg gcaccacggg ctctggtaag tccgtcggcg tcaactcgat gatcacgtcg
     1381 ctgctctatc acgcgtcgcc cgaagacgtt cgcatgatca tggtcgaccc caagatgctc
     1441 gagctctcga tctacgaggg cgtgccgcat ctcctgctac cggtcgtgac cgatccgaag
     1501 aaggcgaacc tcgcgctgcg ctgggccgtc gaggagatgg agcgccgcta cgatctcctc
     1561 gcgcagcagg gcgtccgcga catcatcggc tacaacgaga aggtcgcgaa gcagcaggcc
     1621 aagctcgaag cggacaagct ccgcgaagcg gccgatgcag ccgcaaaggc gctcgaagaa
     1681 gatctcgaga aggtcatctc cgctgactcg ccggaagcgg ccgatgcacg cgagaaagtc
     1741 gagaccgcgc agcaccagca caagctgccg tacatcgtcg tcgtgatcga cgagttcgcc
     1801 gacctcatga tgtgcgcacc gaaggaagtc gagacgagcg tcgcgcgtat cgcgcagaag
     1861 gcgcgcgcgg ccggcatcca cctcatcctc gcgacgcagc gcccgagcgt cgatgtcatc
     1921 accggtctca tcaaggcgaa cttcccgtcg aggatcgcgt tctcggtcgc ctcgaagatc
     1981 gactcgcgca ccatcctcga tcagaacggc gccgaagcat tgctcggtgc aggcgacatg
     2041 ctgttctccg atcgcggcgc atcacccgtg cgtctccacg gctgctacgt cgacgagagc
     2101 gagatccatc gcgtcgtcga tcacctcaag acgcagggcc gcccggtcta caacctcgac
     2161 atcgtgaagc cgcgcgacga agaaggcgaa ggtggcaacg aattcggcgg tggcggcggt
     2221 ggtggcggca aatcggacga cgatatgtac gacaaggccg tgtacatcgt gacgacgacg
     2281 cgcaacgcgt cgatctcgtg ggtccagcgc cagctgcgca tcggctacaa ccgcgccgcg
     2341 cgtctcgtcg aagagatgga gaagcagggc gtcgtgtcgc cgccggatca cacgaacaag
     2401 cgcgaagtgc tcgtggctgc cgcctaattc ccgaggaacg tgaagtacag ctcgggcggg
     2461 tgaccgaaca cgagcgcgtc gccgcagtcg ggacacttca gcgcgggcgc gcgcatacga
     2521 gcgaggtcgt tgccgtaggt gcggacgtcg agcagcatga cgaactcgcg gtcgcattca
     2581 tcgcattcgt acggcgcgta gaacgacgac acgagcgaca cgcctcgcgc agcgggcacg
     2641 atgttgagct gatgcacgat cggctcggcg acgcgccgca gctcgatggc aaccttggcc
     2701 ttcgccgcgg cctgctgcat gtagcaccac tcgcgaacgc cgatcgagtt gatgaacgtg
     2761 atgcccgcga gatcgaggac gaggcggccg ttcttcgctt gcgcgatgag gtcggtcaac
     2821 ttcgccgatt catcgatcga gcctgctagc agcagctgat cggcgttcgg caccaccgtc
     2881 attcgactca cgcgatcaac gttacacgct actcttggcg ggtgcgagcc acctggctca
     2941 ccgacgttca cctcaacttc atgaggccgc tcgcgttgcg cgccttctat gaacgggtgc
     3001 gtcaggaacg tcccgactgc ctgtttgtca ccggcgatat cggggaggca gactccgtcg
     3061 gtcgattcgt cgaggagctc gggcggatcg caccggtcta ctacgtgctc ggcaaccacg
     3121 attattaccg agccggcatt cacgccgttc gcgagaatct cccagcgggg tggctgcctg
     3181 ccgcggggcc cgtcgcgctg accgagcaaa cggtgctgct cggggtcgat ggctggggtg
     3241 acgcgcggtg cggcgacctc gattcgaacg tgcagctctc cgactggcag ctgatcgagg
     3301 acttcgagcg ggtgcgctac gaccgcaagg cgcgcgtcgc gctgcttcag gagctgggca
     3361 ccgctgaagc attcgtgctc ggcgagctgc tcgcacgtgc accggcgagc cagcggcttg
     3421 tcgtgctcac gcatgtgccg ccgtttcccg aagcgtgcgt ctacgatggt gcgcagtccg
     3481 agccggcctg gctgccgtgg ttcacgtgca tcgcgacagg acagctgctc gccgaatatg
     3541 c
//
LOCUS       DATLBZ010000335         5337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8391147, whole genome shotgun sequence.
ACCESSION   DATLBZ010000335 DATLBZ010000000
VERSION     DATLBZ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8391147"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VMZ53_17385"
     CDS             <1..904
                     /locus_tag="VMZ53_17385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HUS30287.1"
                     /translation="ARVLATPATRRLARQLGVEIGRVPATGKHGRVTTDDVRKFKDSP
                     APSHGGGGAKAPAYAPIAIAKGGEEERIPLRGMRKRISESMSRSVNTAAHFTYVEEID
                     MTELVTVREHAKARAAERGLKLNYLPFIVKAVVSGLKKWPMLNASLDETTQEIVRKKY
                     YHIGIAAQGPQGLVVTVVRDADRRSIFDISKEIDRLAEAVRSNTATREELTGSTFTIS
                     SLGKLGGVLATPIINFPEVAIIGVHKIEEKPAVRDGQIVIRHLMNLSISVDHRLADGW
                     DGAMFLQDVKALLEDPTTMFMEMV"
     gene            936..2432
                     /locus_tag="VMZ53_17390"
     CDS             936..2432
                     /locus_tag="VMZ53_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30288.1"
                     /translation="MSTKKGVGPRDHRSEPDEGSTSEKFRVDRVFVPTAPRREPTAPG
                     NTGSGTTPPPRRTGNTMPPVLNTAPGMDDAELALRRQLSRLQRQLADAQRELANKDEE
                     LAAAAEKRVEVQASFDASLSQLRDAHLALEDIRADYQRLAGVEQRLQDAIATADELTH
                     QLERERAERTAIALQFDETTNQFDRARSLWKEETAVLQEHHAAELAKVEVEKRRSIDA
                     AEAAMKVVMERQDKANAEKLVELEAAHDRALATLRGELEPKVAEARNLATEIERLTSE
                     MAALRTEQMREHAERNDLHKWELQQAAEAHATELAALARKHETELAQRNEEAAAKAQA
                     LATAERNAELREQLWEQTTNGLRESQKKLQLELADAKEKLAQEGANRWNIEQRLAAAL
                     EQVEQLTQRQQDFENRVDAAEAEARRNTLDRQRFAAYLEEGLAMLGALPPKDMSTRHQ
                     AATPDEPTDEADASSAVPQRPTMSYEAVAEEDSGAVALPPPPSDDEPT"
     gene            2617..3021
                     /locus_tag="VMZ53_17395"
     CDS             2617..3021
                     /locus_tag="VMZ53_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30289.1"
                     /translation="MRNVLLASAVAFAALLPATGCVPFGCGAYEGADDRVYARNSSEM
                     LIVCGNGGFVANLESQSIEGRIEYNSDGTAIGVKGDDASLAFDWIVGGDTATTPQLGD
                     GAWTYQSLDQTALDHADVLCQDLETRTWWAQQ"
     gene            3075..4247
                     /locus_tag="VMZ53_17400"
     CDS             3075..4247
                     /locus_tag="VMZ53_17400"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUS30290.1"
                     /translation="MALAAASKALSRAMQYFDGDDEAKARGLFQLLREDGTPVDENAL
                     DGFDRAHARQLFEGMLRIRVTDARMMALQRQGRIGFYGEAMGQEAAVVGSAAASKPDD
                     WIVPALREAGVGLYRGMTLDSYIAQIFGNAVDPTKGRQLPCHPIDREHNYVVMSSCVS
                     TQIPHAVGIAMAMQLSGDKGRCAFGFMGDGGTSEPDFHVALNFAGVRRAPCVLICQNN
                     QWAISTPGNIQTASETIAVKGLGYGMESLRVDGNDVFAVYAAVSHAAEKARRGDGPTF
                     VELLTYRVSAHSSSDDPSRYRDEKVTEVWRHQKDPLGRMETFLKARGWLADGEREALA
                     TQLEADVRDAIARAEAAGLPPLESILDDVYEEQPWHLREQLAELLAAPRVKSPHSH"
     gene            4347..>5337
                     /locus_tag="VMZ53_17405"
     CDS             4347..>5337
                     /locus_tag="VMZ53_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30291.1"
                     /translation="MHRFSLLLAFLGSAAAVAGCAHHQNHISVDGAVLGRVVIYRNGV
                     AFYERSARLEDGKVAVHVPRDRVDDFLKSLTVIDKATKQPLSVSLPRQQADDGNYLTM
                     LLETPEKSNAEVLLTYVTDSPAWKPSYRVVVGDNGHVMLESWAIVDNVSGEDWKNVLV
                     GVGSSSALSFRYDLWSVRRVDRDLLEGDRQFAVAPPTGMSPYSESGGEELANIDSTTQ
                     GGVVALSGAAGDSSGVSFSGSSTSTENTYVVDGINTTGLTYGTNTPTGTSAPEPPPVG
                     NSRGSVSDVKSGERLAGVTVVATKKGAPTNNVTAISDENGMYTLGNIDPGVYDL"
BASE COUNT         1032 a         1813 c         1714 g          778 t
ORIGIN      
        1 cgcgcgtgtg ctcgcaacgc cggcgactcg tcggctcgcg cgtcagctcg gcgtcgaaat
       61 cggccgcgtt ccggcaacgg gcaagcacgg ccgcgtgacg accgacgacg ttcgcaagtt
      121 caaggactcg cccgctccgt ctcacggcgg tggtggcgcg aaggctcccg cgtacgcacc
      181 gatcgcgatc gcgaagggcg gcgaagaaga acgcatcccg ctgcgtggca tgcgcaagcg
      241 catctccgag tcgatgtcgc gctcggtgaa caccgccgcg cacttcacgt atgtcgaaga
      301 gatcgacatg acggagctcg tcaccgtccg cgagcatgca aaagcgcgtg cggccgagcg
      361 cgggctcaag ctcaactacc tgccgttcat cgtcaaggcg gtcgtcagcg gcctcaagaa
      421 gtggccgatg ctcaacgcgt cgctcgacga aacgacgcag gaaatcgtcc gcaagaagta
      481 ctaccacatc ggaatcgccg cccagggacc gcaaggcctc gtggtcacgg ttgtccggga
      541 tgcggaccgt cgctcgatct tcgatatctc gaaagagatc gatcgcctgg cggaggccgt
      601 gcggagcaac accgcgacgc gcgaagagct cacgggctcg acgttcacga tcagctccct
      661 cggcaagctc ggcggcgtgc tcgcgacacc gatcatcaac ttccccgaag tcgcgatcat
      721 cggcgtccac aagatcgagg agaagccggc cgtacgcgac ggtcagatcg tcatccgcca
      781 cctgatgaac ctctccatct ccgtcgacca ccgcctcgcc gacggctggg acggggcgat
      841 gttcctccag gacgtcaaag ccctcctcga agacccgacg acgatgttca tggagatggt
      901 ctagccacca tcggcaggcg tatacttcta aggtcgtgtc gaccaagaag ggtgtggggc
      961 cccgtgatca ccgctctgag cccgatgagg gttcgacgag cgagaagttt cgcgtcgacc
     1021 gcgtcttcgt tccaaccgcg ccgcgccgcg agccaactgc gccaggcaac accggcagcg
     1081 gcaccacgcc accgccgcgc cgcaccggca acacgatgcc gcccgtgctc aacacggcgc
     1141 cgggcatgga cgacgccgag ctcgcgcttc gacgccagct ctcgcgcctg caacgccagc
     1201 tcgccgatgc acaacgcgag ctcgcgaaca aagatgaaga gctcgccgcc gcggcagaaa
     1261 agcgcgtcga ggtccaagcg tcgttcgacg cctcactctc gcagctccgc gacgcgcacc
     1321 tcgcgctcga agacatcagg gcggactacc aacgcctcgc gggtgtcgag cagcgcctgc
     1381 aagacgcgat cgctaccgcc gacgagctga cgcaccagct cgaacgcgag cgcgccgaac
     1441 gcacggcgat cgccctccaa ttcgacgaga cgacgaacca attcgaccgc gcacgctccc
     1501 tctggaaaga ggagacggcg gtcttgcaag aacatcacgc cgccgagctc gccaaagtcg
     1561 aggtcgaaaa gcgccgctcg atcgacgccg ccgaagccgc gatgaaagtc gtgatggagc
     1621 ggcaagacaa agccaacgcc gaaaagctgg tcgagctcga agccgcgcac gatcgcgcgc
     1681 tcgccaccct ccgcggcgag ctcgagccca aagtcgccga agcgcgcaac ctcgccaccg
     1741 aaatcgaacg cctcacctcc gaaatggccg cgctgcgcac cgaacaaatg cgcgagcacg
     1801 ccgaacgcaa cgacctccac aagtgggagc tccaacaagc cgccgaagcc cacgccaccg
     1861 agctcgccgc gctcgcccgc aagcacgaga ccgagctcgc acagcgtaac gaggaggcgg
     1921 ccgcgaaagc ccaagcactc gccaccgccg agcgcaacgc cgagctccgc gagcagctct
     1981 gggaacaaac gacgaacggc ctccgcgaga gccaaaagaa gctccaactc gagctcgccg
     2041 acgccaaaga gaagctcgcg caagaaggcg cgaaccggtg gaacatcgag cagcgcctcg
     2101 ctgccgcgct cgagcaggtc gaacagctca cgcaacgcca gcaagatttc gagaaccgcg
     2161 tcgacgccgc cgaagccgaa gcccgccgca acacgctcga ccgccagcgc ttcgcggcct
     2221 acctcgaaga gggcctcgcg atgctcggcg cgctgccacc caaagacatg tcgacgcgac
     2281 accaggccgc gacgcccgac gagccgacgg acgaggcgga cgcgtcgagc gctgtgccgc
     2341 aacggccaac gatgtcgtac gaagccgtcg ccgaagaaga ctccggggcc gtcgccctcc
     2401 cgccgccgcc gagtgacgac gagccgacct agatcgtccg cgcgtaaatc aagtccgacc
     2461 taatttccga gccgaacgca gcgtaatgac gtcggactcg atttccgggg catgacagcc
     2521 atgtcagggc ctggcaaaag ccagccgagt ggcggcgcgt aagttgccgg aatgtgcggt
     2581 ggaactgttg gtcccgacgt tgcgatatgg ggaggcatgc gcaacgtcct cctcgcctcg
     2641 gctgtcgcct ttgccgctct cctgcccgct actggctgcg tcccctttgg ttgtggcgcg
     2701 tacgagggcg cggatgaccg cgtctacgcc cgcaacagct ccgagatgct gatcgtctgc
     2761 ggcaacggcg gcttcgtggc gaacttggag tcgcagagca tcgaaggccg catcgagtac
     2821 aacagcgacg gaaccgcgat tggcgtgaag ggcgacgatg cgtcgctcgc gtttgactgg
     2881 atcgttggcg gcgataccgc gaccacgccg cagctcggcg atggcgcgtg gacgtatcag
     2941 agcttggatc agacggcgct cgaccacgcc gacgtccttt gtcaggacct cgaaacccgc
     3001 acgtggtggg cgcagcagta gacgcgtctt agcggcttgc cttccgccgc ggctctcgcg
     3061 ctatggtgcg ggggatggca ctcgccgcag cttcgaaagc gctgtcccgc gcgatgcagt
     3121 atttcgacgg tgacgacgag gcgaaggcac gcggtttgtt ccagcttctg cgcgaggacg
     3181 gcacgcccgt cgacgagaac gcgctcgatg gctttgatcg cgcgcacgca cgccagctct
     3241 tcgaaggcat gctgcgaatt cgcgtgaccg atgcgcgcat gatggcgctg cagcgtcagg
     3301 gccgcatcgg cttttatggc gaagcgatgg ggcaagaagc ggcggtcgtt ggatcggctg
     3361 cggcgagcaa gcccgatgac tggatcgtgc cggcgttgcg cgaggccggc gtcgggctgt
     3421 atcgcggcat gacgctcgac agctacatcg cgcagatttt cggcaacgcg gtcgatccga
     3481 cgaagggtcg ccagctgccg tgtcatccca tcgatcgcga gcacaactac gtcgtgatgt
     3541 cgtcgtgcgt gtcgacgcag atcccgcacg cggtcggcat tgcgatggcg atgcagctgt
     3601 ccggcgacaa gggccgctgc gcgttcggct tcatgggcga tggcggcacg agcgagccgg
     3661 acttccacgt cgcgctgaac tttgcgggcg tgcgacgcgc gccgtgcgtg ctgatttgcc
     3721 agaacaacca gtgggcgatt tccacgcccg gcaacatcca gacagcgtcg gagacgatcg
     3781 cggtcaaggg cctcggctac ggcatggagt cgctccgtgt cgacggcaat gacgtgttcg
     3841 cggtctacgc cgccgtcagt cacgccgccg agaaggcacg ccgcggcgac ggtcccacgt
     3901 tcgtcgagct gctcacgtat cgcgtgagcg cgcatagctc gtcggacgat ccatcgcggt
     3961 atcgcgacga gaaggtcacc gaagtgtggc gtcaccagaa ggatccgctg ggccgcatgg
     4021 agacgttcct caaggcgcgc ggctggctcg ccgatggtga gcgcgaggca ctcgcgacgc
     4081 agctcgaggc cgatgtccgc gatgcgatcg cgcgcgccga agcagctggc ctgcccccgc
     4141 tcgagtcgat tctcgacgac gtttacgaag aacagccgtg gcatttgcgc gagcagctcg
     4201 ccgagctatt ggccgccccg cgcgtgaagt cgccgcactc gcactagctg cctgcgacag
     4261 cctgcagggt gcgacaccgc tgcgtctaac agcgagtcag cagcgcagca tggcgtgagc
     4321 ggtcccaagc cgcgtatcga tgaagcatgc atcgcttctc gctgctgctc gccttcctcg
     4381 gttccgcggc cgccgttgcc ggttgcgccc accaccagaa ccacatctcc gtcgatggcg
     4441 ccgtgctcgg ccgcgtcgtc atctaccgaa acggtgtggc gttctacgag cgctcggcgc
     4501 gcctcgagga cggcaaggtc gcagtacacg tgccgcgcga tcgcgtggac gacttcctca
     4561 agtcgctgac cgtcatcgac aaggcgacga agcagccgct ctccgtctcc ctcccgcgcc
     4621 agcaggccga cgacggcaac tacctcacga tgctgctcga gacgcccgag aagtccaacg
     4681 ccgaggtgct gctcacgtac gtcaccgatt cgccggcgtg gaagccgagc tatcgcgtcg
     4741 tcgtcggcga caacggccac gtcatgctcg agagctgggc gatcgtcgac aacgtctcgg
     4801 gcgaagactg gaagaacgtc ctcgtcggtg tcggctcgag ctcggcgctg tcgtttcgct
     4861 acgacctgtg gagtgtgcgc cgcgtcgatc gcgatctgct cgaaggcgat cggcaattcg
     4921 cggtcgcgcc gccgacgggc atgtcaccgt acagcgaaag cggcggcgaa gagctcgcaa
     4981 acatcgactc gacgacgcaa ggcggcgtgg tggctctctc gggtgccgcg ggagactcat
     5041 cgggcgtttc gttcagtggc agctcgacga gcacggagaa cacatacgtc gtcgacggca
     5101 tcaacacgac gggcctcacc tatggcacga acacaccgac gggcacgagc gcaccggagc
     5161 cgccgcccgt tggcaacagt cgcgggtcgg tcagcgatgt gaagagcgga gagaggctcg
     5221 ccggcgtcac cgtcgtcgcg acgaagaagg gcgcaccgac caacaacgtc acggcgatct
     5281 ccgacgagaa cggcatgtac acgctcggca atatcgatcc cggcgtctac gacctcg
//
LOCUS       DATLBZ010000336        10018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9329128, whole genome shotgun sequence.
ACCESSION   DATLBZ010000336 DATLBZ010000000
VERSION     DATLBZ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9329128"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VMZ53_17410"
     CDS             complement(<1..1023)
                     /locus_tag="VMZ53_17410"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HUS30292.1"
                     /translation="MSGTFQKRLLAGLFVMLIVIIGGAGGYYAIGRGQWAFFDCLYMT
                     IITVTTVGYGETLPGMETVPYARGFTMLLLLFGTGSIVFFASTITAVIVEGDLKNALF
                     ANKLKKRMKRMKDHVIVCGAGSTGRNVIEELLKTGVPVIAIDVNEAELRDIADKHPNA
                     EYSFIVGDATDDDVMAQTNLSTARGLVAALSSDKDNLYLTVSARQDNPKARIVARCAE
                     LSHVEKIKKSGADAVVSPNYIGGLRLVSELVRPSVVRFLDEMMRDRRAAYRIEEVTLG
                     DGITALGSTLRDARVRERFGMTVLAVRKPTDDAWNYNPDATVTIGPGTTLVVLGSSEQ
                     VAELRRQ"
     gene            1065..2612
                     /locus_tag="VMZ53_17415"
     CDS             1065..2612
                     /locus_tag="VMZ53_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dehydroadipyl-CoA semialdehyde
                     dehydrogenase"
                     /protein_id="HUS30293.1"
                     /translation="MQLLSSHLSGAWSPGSGSRTPLVNPATEEVLAEVSAGGHDLAGA
                     FKYAREVGGNELAGMTFAQRGALLGALAKAIHGAREELISLAVANGGNTRGDAKFDID
                     GGAVTLSHYAELGAKLGGSSLLADGEPIQVGRTARMGGQHVWARRRGVAVHINAFNFP
                     AWGTCEKLACSLLAGMPVITKPATATALVAHRLAQLFEPLLPKGVLQTLIGPAGSLLD
                     SLAPGDVVAFTGGSSTAKTLRTHAKILEHGVRLNIEADSLNAAILGPDIELSSEAGQL
                     FLVDVVRDMTQKTGQKCTAIRRVLVPDARFAEVCDAIRERVSTIAIGDPSREDVRMGP
                     VATAAQRDDVKAGIARLAAATDSVHGGNGEVTPIGVPAGKGFFVGPVVRSTKDPMNCA
                     PLNSHEVFGPVTTVGAYTGDAALAAAFVARGEGCLVSSAYSDDRDWVQAFVTAAAPWA
                     GRLYLGSSKMASQSPGPGTVLPSLLHGGPGRAGGGEELGGERGLHFYMQRCALEGDAS
                     VLKSLLG"
     gene            2609..2989
                     /locus_tag="VMZ53_17420"
     CDS             2609..2989
                     /locus_tag="VMZ53_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30294.1"
                     /translation="MRIAIVGAVLAIVVALALVFTNTGRAPAPPVDPQHQAAAIQVAR
                     CDAAMSKLDAELKTSKAASALATRIEVCTKARAAVERAQTNKPSTQLEVALARVNKHL
                     ARLNEGMGSAAPAPSPTAGSAGSN"
     gene            complement(2986..3507)
                     /locus_tag="VMZ53_17425"
     CDS             complement(2986..3507)
                     /locus_tag="VMZ53_17425"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUS30295.1"
                     /translation="MANIFELIDKKDVEGLRTYLSIEPSASGQRDPQGLSVLMRAFYR
                     SPELVEVVRKSDPPLDEWDRLLIGESKGIPPHDAWSPDGFTPLHIAAFAKNMAACKAL
                     IADGADPNVIAKAHFAKVTPLGTCAFVNAIEIARLLLDAGANPKIPEGASPIDSARHN
                     KHSELLALLESRA"
     gene            complement(3541..4254)
                     /locus_tag="VMZ53_17430"
     CDS             complement(3541..4254)
                     /locus_tag="VMZ53_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30296.1"
                     /translation="MSTIDDEVRDALKELVEEIGATSARIVDEDSDPRVGVPARTLAL
                     GGGEYLRVELPTRVERETGNEHDIESAFERITRTLRGIRRRYEVARLPEVSFAPGTLP
                     PGGKVLERIESYMQALAQIDQASNAFVTRGTRLIASAKPPDEMEGSRWPFLARRALST
                     HAPGSSHGEVVDPDAFAMSFWYDAALVVLMNGPYGVDFVRHRCKQVARELCNLIPMLD
                     PDPEAPAAIGPRPRRPTQP"
     gene            complement(4251..4928)
                     /locus_tag="VMZ53_17435"
     CDS             complement(4251..4928)
                     /locus_tag="VMZ53_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30297.1"
                     /translation="MGGCTNCKGKTGCDHRKGEMLESVDRALEMLYPTRTWGEPDDAA
                     ANGMPPDELAALADELAQELGAATFVREGGDDEPCDYIYVMCMGRTPCVVQARDHGVA
                     IPAEWRDVDGLEELYLRVVISQRARVAAVQQVALQLVRNGDQFLVRETPRAGVYDAPL
                     LRRMQKLVAILPAYELLHVDFGEIAHAPPGYNAGPWRELFGGEPSIANYLFYPQPTTM
                     ITTSALA"
     gene            complement(4955..6199)
                     /locus_tag="VMZ53_17440"
     CDS             complement(4955..6199)
                     /locus_tag="VMZ53_17440"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HUS30298.1"
                     /translation="MLIVQKYGGTSVGTIERMQNVAKRCLEAQRKGHKVAVVVSAMSG
                     ETNRLLKLVDQIHDDHNDREVDVIVSTGEQVSVGLVALSIRKLGGKARSFLGHQCKIM
                     TDSSFARARIVDIDSKPIRDAMDNGEIAVIAGFQGVDEKGNITTLGRGGSDTTGVAIA
                     AALKADVCEIYTDVDGVYTTDPNVVPTARKIDRISYEEMLELASLGAKVLQIRSVELG
                     MKYGVPIHVRSSFSDVEGTWVVPEEASMEKVVVSGVTASRDESKITCEDLPDTPGIAA
                     RMFAPLADANISIDMIVQNQAYDGTTDLTFTLPKIDRKRAIDLMKEKVPELVGKDGER
                     VSFDDEISKVSVVGVGMRSHAGIAKTMFTLLAAEGINIQLISTSEIKISCVIARKYAE
                     LAVRTLHEGFGLSLPESERTSL"
     gene            6231..6551
                     /gene="cutA"
                     /locus_tag="VMZ53_17445"
     CDS             6231..6551
                     /gene="cutA"
                     /locus_tag="VMZ53_17445"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HUS30299.1"
                     /translation="MSVVVAFSTFPSPEKAAEVVRALVTEQLAACGNLVSPVRSIYRW
                     KGELQDDTETLAIIKTTRERFEEMKARLVELHPYEVAEVICMPVEAGNVPYLDWVEAE
                     TKKP"
     gene            complement(6552..7052)
                     /locus_tag="VMZ53_17450"
     CDS             complement(6552..7052)
                     /locus_tag="VMZ53_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30300.1"
                     /translation="MSLAFAVACADSPGTGDDDQGDDTSGNTCGDGTCATAEIGYCPT
                     DCGDGNGNNGNNAVCGNGQCETTKGESASTCFSDCGGNNNGSGSGQQSGSCPADPNAC
                     IGCLLDPSQCPSGLDMNSCLQCVLNGGGLGSGFPGGSGLGSGACNFNQVCDSGEDATG
                     CPTDCP"
     gene            complement(7163..9919)
                     /gene="recG"
                     /locus_tag="VMZ53_17455"
     CDS             complement(7163..9919)
                     /gene="recG"
                     /locus_tag="VMZ53_17455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HUS30301.1"
                     /translation="MDAELKRRVEALRRPLELAAANDFAGVRRVQGLGNVLRIACDGL
                     MQRWSSEQLAAWRIVLAKWEQLPIAQQEAEVARGMRLIARLPSAPSLASAKATIEDIA
                     AKAMAAKSMVAKPTSGAPSSAMVAKSTSAAALNAKASGEKASGEKASGEKASSEKASS
                     EKARAKGPRAAAAETIADPLGAPTHTLPGIGPAFAEKLAEKNLETVEDLLWCLPRRYD
                     DVRDAKQLAEVCEMDEGMRATFSAKVASARMVFVRRRRWAEVRLGAVDVTQPASCVVR
                     WFNVWAGIEKRMPAGSVVTLSGVVKKRSGRLELANPDILAIDVADAQLPLDAASDGSI
                     PKAKKAPPTIIPRYPEVQGVPASRLRVACQTACARVGASADDGVPKSIEAASGMPSLA
                     ETLARLHDPPSDITVEDLAALNRGDSPWQRRLAFGELFALGVAVAMRRKERRADAAVA
                     CARTPGLDDAVAKALPFRMTTAQQRAVREMGDDLARPVPMNRLLQGDVGAGKTAVAFA
                     AALQVARAGRQTAVMAPTELLAEQHFETWKKWAAVTGLRVELLTASTQKGVRQTLLAL
                     LAAGKIDVLLGTHSLIAEAVGFAALGLVVIDEQHRFGVAQRAKLRDKGDGQGAPHLLV
                     MTATPIPRTLALTAYGDLDVSILDELPPGREPAKTKVVAGKRGEQAAYKLIAERVAAG
                     ERAYVVCPKVEMADEHDENAKDWKDATSVASEMAAALPQLRVGLVHGRLDSEARDRVM
                     RAFKQGELDVLVATTVIEVGVDVPSATVMVIHDAERFGLAQLHQLRGRVGRGGGAAHC
                     VLVTGGRSGAKPANAGERGSISEDGRRRLDAMVRTTDGFLIAEEDLALRGPGELLGPR
                     QAGIPRLRFGSLAQHTELLLEARGHAERILDEDPGLVRPEHAALRAALARRLAQSVYG
                     AESG"
BASE COUNT         1604 a         3503 c         3216 g         1695 t
ORIGIN      
        1 ctggcggcgt agctctgcga cttgctcgga tgagcccagg acgacgagcg tcgtgcccgg
       61 accgatcgtg acggtagcgt cggggttgta gttccaagcg tcgtcggtgg gcttgcgcac
      121 ggcaagcacg gtcatgccga agcgctcgcg gacgcgtgcg tcgcgcaggg tcgagccgag
      181 cgcggtgatg ccatcgccga gggtgacctc ttcgatgcgg taggcggcgc gccgatcgcg
      241 catcatctcg tcgaggaagc gcacgacgga ggggcgcacg agctcggaga cgaggcgaag
      301 gccgccgatg tagttcggcg agacgacggc gtcggcgccg gacttcttga tcttctcgac
      361 gtgcgagagc tcggcgcacc gcgcgacgat gcgcgccttc gggttgtctt ggcgtgccga
      421 gacggtgagg tagaggttgt ccttgtcgga ggagagcgct gcgacgaggc cgcgtgccgt
      481 cgagaggttg gtctgcgcca tcacgtcgtc gtcggtggcg tcgccgacga tgaacgagta
      541 ctcggcgttc ggatgcttgt cggcgatgtc gcgaagctcg gcttcgttga cgtcgatcgc
      601 gatgaccgga acgccggtct tgagaagctc ttcgatgacg tttcggcccg tcgagcccgc
      661 gccgcacacg atgacgtggt ccttcattcg cttcatccgt ttcttgagct tgttcgcgaa
      721 cagcgcattc ttgagatcac cctcgacgat cacggccgta atggtcgatg cgaagaacac
      781 gatgctaccc gtgccgaaca ggagcaggag catcgtgaag ccgcgggcgt acgggacggt
      841 ttccatcccg ggtagggttt cgccgtagcc gaccgtcgtg accgtgatga tggtcatgta
      901 gaggcagtcg aagaaggccc actggccgcg gccgatggcg taatagccgc cggcgcctcc
      961 gatgatgacg atgagcatca cgaagaggcc tgccaacagg cgtttctgga acgtccccga
     1021 catcggtcca gcgcgacgat atcaggtctg ataaaaagcc gaccatgcag ctgctctcca
     1081 gccacctgtc cggggcctgg tctccgggat cgggatcgcg cacgccgctc gtcaaccccg
     1141 cgaccgaaga ggtcctggcc gaggtctcgg ccggcgggca tgacctggcc ggcgccttca
     1201 agtatgcgcg cgaggtcggc ggcaacgagc tggccggcat gacgttcgcc cagcgcggcg
     1261 cgctcctcgg cgccctcgcg aaagcgatcc atggcgcacg cgaagagctc atctcgctcg
     1321 cagtcgcgaa cggcggcaac acgcgcggcg acgcgaagtt cgacatcgac ggcggcgctg
     1381 tcaccctctc ccactacgcc gagctcggcg ccaagctggg tggctcgagc ctgcttgccg
     1441 acggcgagcc gattcaagtc ggtcggaccg cgcgcatggg tggccagcac gtgtgggcgc
     1501 gtcggcgtgg cgtcgccgtt cacatcaacg cgttcaactt cccggcgtgg ggcacgtgcg
     1561 agaagctggc gtgctcgctg ctcgcaggca tgcccgtgat cacgaagccg gcgacggcga
     1621 ccgcgctcgt cgcgcatcgg ctcgcgcaac tgttcgagcc gctgctgccg aagggcgtgc
     1681 tccaaacgct gatcggcccc gcgggctcgc tgctcgacag cctcgcacca ggcgacgtcg
     1741 tcgcattcac gggcggcagc tcgaccgcga agacgctccg cacgcacgcc aagatcctcg
     1801 agcacggcgt ccgcctcaac atcgaggccg actcgctcaa cgctgcgatc ctcggccccg
     1861 atatcgagct gtcgagcgaa gcaggccagc tgttcctcgt cgatgtcgtg cgcgacatga
     1921 cgcagaagac cggccaaaaa tgcaccgcga tccgccgcgt gctcgtcccc gacgcacggt
     1981 tcgcagaagt ctgcgacgcg atccgtgagc gcgtgtcgac gatcgcgatt ggcgatccat
     2041 cgcgtgaaga cgtccgcatg gggccagtcg ccaccgccgc tcagcgcgac gacgtcaagg
     2101 ccggcatcgc gcgcctcgcc gccgcaaccg atagcgtcca cggtggcaac ggcgaggtca
     2161 cgccgatcgg cgttccggca ggcaaaggct tcttcgtcgg cccggtcgtc cgttcgacga
     2221 aggatccaat gaattgcgcc ccgctcaact cgcacgaagt cttcggcccc gtgacgacgg
     2281 ttggcgcgta caccggcgat gccgccctcg ctgccgcgtt cgtcgcgcgt ggcgaaggct
     2341 gcctcgtctc gagcgcgtac tccgacgatc gcgactgggt gcaggcattc gtcacggccg
     2401 ctgctccgtg ggccggccgc ctctacctcg gctcatcgaa gatggcgtcg caatcacccg
     2461 gtccgggcac cgtcctgccg tcgctgctgc acggcggccc cggccgcgcc ggtggtggcg
     2521 aagagctcgg cggcgagcgc ggcttgcact tctacatgca gcgttgcgcg ctcgaaggcg
     2581 atgcaagcgt gctgaaatcg ctgctcggat gaggatcgca atcgttggcg ccgtgctcgc
     2641 gatcgtggtc gcgctcgcgc tcgtgttcac gaacacgggt cgcgcgccgg ccccgcctgt
     2701 cgatccgcaa caccaagccg ccgcgatcca ggtcgcgcgc tgcgacgccg cgatgtcgaa
     2761 gctcgatgcc gagctgaaga cgagcaaggc cgcgagcgcg ctcgcaacgc gcatcgaggt
     2821 ctgcacgaaa gcccgcgccg cggtcgagcg cgcgcaaaca aacaagccga gcacgcagct
     2881 cgaagtcgcg ctcgcgcgcg tcaacaagca cctcgcccgc ctcaacgaag gtatgggctc
     2941 ggccgcacct gccccttcgc ccacagcagg ctcggccggt tcaaactagg cgcgcgactc
     3001 gaggagcgcg agcagctcgc tgtgcttgtt gtggcgcgct gaatcgatcg gcgaggcgcc
     3061 ttcgggaatc tttgggttgg cgcccgcgtc gagaagcaag cgcgcgatct cgatcgcgtt
     3121 gacgaacgcg catgtgccga gcggggtgac cttggcgaag tgcgcctttg cgatcacgtt
     3181 ggggtcggcg ccgtccgcga tgagcgcctt gcaagcggcc atgttcttcg cgaacgccgc
     3241 gatgtggagc ggtgtgaaac cgtcgggcga ccacgcatcg tgcggcggga ttcctttcga
     3301 ctcaccgatc agaaggcgat cccattcgtc gagcggcggg tcagacttgc gcacgacctc
     3361 gacgagctcg gggctgcgat agaacgcgcg catcaagacc gacaggcctt gtggatcgcg
     3421 ctgaccactc gccgacggct cgatggagag gtacgtgcga agtccctcga cgtccttctt
     3481 gtcgatcagc tcgaagatgt ttgccacgca gctagcgtat caaagcggcg atgtcgcggc
     3541 tcagggctgc gtcgggcggc gcggccgcgg accgatggcg gcgggagctt cgggatccgg
     3601 gtcgagcatc ggaatgagat tgcacagctc gcgcgcgact tgcttgcagc ggtggcgcac
     3661 gaagtcgacg ccgtacggcc cgttcatgag cacgacgagc gctgcgtcgt accagaagct
     3721 catcgcgaac gcgtcgggat cgacgacctc gccgtgcgag ctcccggggg cgtgcgtcga
     3781 gagagcgcgg cgcgcgagga acggccagcg cgagccttcc atctcgtcgg gcggcttcgc
     3841 cgatgcgatg aggcgcgtgc cgcgcgtgac gaacgcgttc gacgcttgat cgatctgcgc
     3901 gagcgcctgc atgtacgact cgatccgttc gagcaccttg ccacctggcg gcagcgtgcc
     3961 cggcgcgaag ctcacttcgg gcaggcgcgc gacttcgtag cgccggcgga tgccgcgcag
     4021 cgtgcgcgtg atgcgctcga aggcggactc gatgtcgtgc tcgttgccgg tctcgcgctc
     4081 gacgcgggtg ggcagctcga cgcgcagata ttcgccaccg ccgagcgcga gcgtgcgcgc
     4141 cgggacgccg acgcgggggt cggagtcttc atcgacgatg cgcgcggacg tcgcgccgat
     4201 ttcctcgacg agctccttga gagcgtcgcg cacttcgtcg tcgatcgtgc tcatgccagg
     4261 gcgctcgtcg tgatcatcgt cgtgggctgc ggatagaaga gatagttcgc gatcgatggc
     4321 tcgccaccga acagctcgcg ccagggacct gcgttgtacc ccggaggggc atgcgcgatc
     4381 tcgccaaaat cgacgtgcag cagctcgtac gccggcagga tcgcgaccag cttctgcatg
     4441 cggcgaagca gcggcgcatc gtagacaccg gcgcgcgggg tctcgcggac gaggaattgg
     4501 tcgccgttgc gaaccagctg aagcgcgacc tgctggactg ccgccactcg ggcgcgctgg
     4561 ctgatgacga cgcgcagata gagctcttcg aggccgtcca cgtcgcgcca ctcggccgga
     4621 atcgcgacgc cgtgatcgcg agcctgtacc acgcacggcg tccgccccat gcacatcacg
     4681 tagatgtagt cgcacggctc gtcgtcgccg ccttcgcgga cgaacgtcgc ggcgcctagc
     4741 tcttgcgcga gctcgtcggc aagcgcggct agctcgtcgg gcggcatgcc gttcgcggcc
     4801 gcgtcgtcgg gctcgcccca cgtgcgcgtc ggatagagca tctcgagcgc gcggtcgacc
     4861 gactcgagca tttcgccctt gcggtgatcg cagcccgtct tgcccttgca gttcgtgcag
     4921 ccgcccatgg cccgtacctt acccggtgac tcccttagag actcgtgcgt tcgctctcgg
     4981 gcagcgagag tccgaaacct tcgtgcagcg tgcggacggc cagctccgcg tacttgcggg
     5041 cgatcacgca gctgatcttg atctccgacg tcgagatgag ctggatgttg atgccttccg
     5101 ccgcgagcag cgtgaacatc gtcttggcga tgccggcgtg cgagcgcatg cctacgccga
     5161 cgacggagac ttttgagatt tcgtcgtcga agctgacgcg ctcgccgtct ttgccgacga
     5221 gctcgggcac cttctccttc atgagatcga tggcgcgctt gcgatcgatc ttggggagcg
     5281 tgaacgtcag gtccgtcgtg ccgtcgtacg cctggttctg aacgatcata tcgatcgaga
     5341 tgttggcgtc ggcgagcggc gcgaacatgc gagccgcgat gccgggcgta tccggcaagt
     5401 cctcgcacgt gatcttcgat tcgtcacgcg aagcggtgac accagagacg acgacttttt
     5461 ccatggatgc ctcttcggga acgacccacg ttccctcgac gtcggaaaag ctcgagcgga
     5521 cgtgaatcgg cacgccgtac ttcatcccga gctcgaccga gcgaatctgc aagactttcg
     5581 cgccgagcga cgcgagctcg agcatttctt cgtagctgat gcggtcgatc ttgcgcgcgg
     5641 tcgggaccac gttcggatcg gtcgtgtaga cgccgtcgac gtccgtatag atctcgcaga
     5701 cgtcggcctt gagcgcggcc gcgatcgcga cgcccgtcgt gtccgagccg ccgcggccga
     5761 gcgtcgtgat gttgcccttc tcgtcgacgc cctggaaccc ggcgatgacc gcgatctcgc
     5821 cgttgtccat cgcatcgcga atcggcttgg agtcgatgtc gacgatgcgc gcgcgtgcga
     5881 acgagctgtc cgtcatgatc ttgcactggt ggccgaggaa cgagcgcgcc ttgccgccga
     5941 gcttgcgaat cgacagcgcg acgaggccga ccgacacctg ctcgccggtc gagacgatga
     6001 cgtcgacctc gcgatcgttg tggtcgtcgt ggatttggtc gacgagcttg agcagccggt
     6061 ttgtctcgcc gctcatggcc gacacgacga ccgccacctt gtggcccttg cgctgggcct
     6121 cgaggcatcg cttcgcgacg ttctgcatgc gctcgatcgt gccgacggac gttccgccgt
     6181 acttctgaac gatcaacaca gtgcgtcact ctacatgata gagacggggc atgtccgtcg
     6241 tcgtcgcgtt ttcgacgttc ccgtctcccg agaaggccgc cgaggtcgtg cgagcgctcg
     6301 tcaccgaaca gctcgcggcg tgcggaaacc tcgtcagtcc cgtgcgctcg atctatcgct
     6361 ggaagggcga gctgcaggac gacaccgaaa cgctcgccat catcaagacg acgcgcgagc
     6421 gcttcgagga gatgaaggcg cgactcgtcg agctacaccc gtacgaagtc gcagaggtca
     6481 tctgcatgcc ggtcgaagcc ggcaacgttc cgtacctcga ttgggtcgag gccgagacga
     6541 agaagccctg attaggggca gtccgtcgga cagccggtcg cgtcttcgcc cgagtcgcaa
     6601 acctggttga agttgcacgc gcccgagccg aggcccgagc cgcccgggaa gcccgagccg
     6661 aggccgccgc cgttgagcac gcactgcaag cagctgttca tgtcgaggcc cgaggggcac
     6721 tgcgacggat cgaggaggca gccgatgcac gcgttcggat cagccgggca cgagccgctc
     6781 tgctgtccgc tgccgctgcc gttgttgtta ccgccgcagt ccgagaagca cgtcgaagcg
     6841 ctctcgccct tcgtcgtctc gcactggccg ttgccacaga cggcgttgtt gccgttgttg
     6901 ccgttgccat cgccgcagtc ggtcgggcag tacccgatct cagcggtcgc acacgtgccg
     6961 tcgccgcacg tgttgcccga ggtgtcatcg ccttgatcgt catcgccggt gccgggggag
     7021 tcggcacacg caacagcgaa cgcgagcgac atcgacagag cgcagaagat ccccgatttc
     7081 atcctcatca tcgcggcgca tcgtttaccg agcaggcgtt gtcgatcaag gacaaaatga
     7141 gcgcgactcg cgccaaacac gatcagccgc tttcagcgcc gtacacagat tgtgcaagtc
     7201 tgcgggctaa cgccgcgcgc aacgccgcat gctccggtcg gaccaagccc ggatcctcgt
     7261 cgaggattcg ctccgcgtga ccgcgagcct cgagtagcag ctccgtatgc tgggcgagac
     7321 tgccgaaccg cagcctcggg atcccggcct gcctcggccc gagcagctcg cccggcccgc
     7381 gcagcgcgag gtcctcctcc gcgatcaaga aaccgtccgt cgtccggacc atcgcatcca
     7441 gccgccgccg cccgtcctcg ctgatcgatc cccgctcgcc agcgttcgct ggcttcgctc
     7501 cactccggcc accggtgacg agcacgcagt gcgccgcccc accgccgcgc ccgacacgtc
     7561 cgcgcaactg atgcagctgc gcgagcccaa atctctccgc atcgtggatg accatcacgg
     7621 tcgcgctcgg cacatcgacg ccgacttcga tcacggtcgt cgcgacgagc acgtcgagct
     7681 cgccttgctt gaacgcacgc atcacgcgat cgcgcgcctc actgtcgagt cgtccgtgca
     7741 cgagcccgac gcgcagctgc ggcagcgccg ctgccatctc gctcgcgacg ctcgtcgcgt
     7801 ccttccagtc tttcgcgttc tcgtcatgtt cgtcggccat ctcgaccttc gggcacacga
     7861 catacgcgcg ctcacccgcc gcgacgcgct ccgcgatgag cttgtacgcc gcctgctcgc
     7921 cgcgctttcc cgccacgacc ttcgtcttcg caggctcgcg acctggcggc agctcatcga
     7981 gaatcgagac atccaaatcg ccatacgcgg tcagcgccaa cgtccgcgga atcggcgtgg
     8041 ccgtcatgac caacaaatgc ggcgcgccct ggccgtcgcc cttgtcacgc aacttcgcgc
     8101 gctgcgcgac gccgaaccga tgctgctcgt cgatcacgac aagcccgagc gccgcaaacc
     8161 cgaccgcctc cgcaatgagc gagtgcgtgc cgagcaacac gtcgatcttg cccgcagcca
     8221 acagcgccag cagcgtctgc cgcacgccct tctgcgtcga cgcggtcaac agctccacac
     8281 gcagcccagt caccgccgcc cacttcttcc acgtctcgaa gtgctgctcg gccaagagct
     8341 ccgtcggcgc catcacggcc gtctgccgcc ctgcccttgc aacttgcaac gccgctgcaa
     8401 acgcgaccgc cgtcttgccc gcccccacat cgccttgcag caaccgattc attggcaccg
     8461 gcctcgccaa gtcgtcgccc atctctcgca ccgcgcgctg ctgcgccgtc gtcatgcgaa
     8521 acggcaacgc cttcgcaacg gcgtcatcca accccggcgt cctcgcacac gcgaccgcgg
     8581 catcggcgcg ccgctccttg cgccgcatgg caaccgccac acccaacgca aacagctcac
     8641 caaacgcgag ccgacgctgc cacggactat cgccgcgatt gagcgccgcc aaatcctcga
     8701 cggtaatatc gctcgggggg tcgtgcagcc gcgcgagcgt ctccgccaag ctcggcatgc
     8761 ccgacgccgc ttcgatcgac ttcggcacgc cgtcgtcggc gctcgccccg acgcgcgcgc
     8821 acgccgtctg acacgcgacg cgcaacctcg acgccggcac gccctgcacc tcgggatatc
     8881 gcgggatgat cgtcggcggt gccttcttcg ccttcggtat gctgccgtcc gaagctgcat
     8941 cgagaggcag ctgcgcatcc gcaacatcga tcgcgagaat gtcggggttc gcgagctcga
     9001 gccgcccact gcgcttcttc acgacgcccg acagcgtcac gacgctgccc gccggcattc
     9061 gcttctcgat gcccgcccac acgttgaacc atcgaacgac acagctcgcc ggctgcgtca
     9121 cgtcgaccgc gccgaggcgc acctccgccc agcgtcgccg gcgcacgaac accatgcgcg
     9181 cgctcgcgac cttcgccgag aatgtcgcgc gcatgccctc gtccatctcg cagacttcgg
     9241 cgagctgctt cgcgtcgcgc acatcgtcgt agcggcgcgg caagcaccag agcaaatcct
     9301 cgacggtctc gaggttcttc tccgcgagct tctccgcaaa cgcgggcccg atgccaggca
     9361 gcgtatgcgt cggcgcgcca agcggatccg cgatcgtctc ggccgccgcc gcccgcgggc
     9421 ccttcgcccg cgccttctca ctgctcgcct tctcactgct cgccttctca ccactcgcct
     9481 tctcaccact cgccttctca ccactcgcct tcgcattcaa cgccgccgcg cttgtcgact
     9541 tcgcgaccat cgcgctcgac ggcgcgccgc ttgtcggctt cgccaccatc gacttcgcgg
     9601 ccatcgcctt cgcagcgatg tcctcgatgg tcgctttcgc cgacgcgagc gacggcgcgg
     9661 acggcagccg cgcaatgagc cgcatgccgc gagccacttc cgcttcttgc tgcgcgatgg
     9721 gcagctgctc ccacttcgcg agcacgatgc gccacgccgc gagttgctca ctactccagc
     9781 gctgcatcag cccatcgcac gcgatgcgca gcacgttgcc gaggccttgc acccggcgga
     9841 cgccggcgaa gtcgttcgcc gcggcgagct cgagcgggcg tcgtagcgcc tcgacgcggc
     9901 gcttcagctc ggcatccacg cctcgaccgt aacccgacag tctgactaca atgcgtcgat
     9961 gcgggcgatg gtgttgcttg tggcgatcgc gttgggagca tgcttcacca ccgcgggc
//
LOCUS       DATLBZ010000337         5208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4858899, whole genome shotgun sequence.
ACCESSION   DATLBZ010000337 DATLBZ010000000
VERSION     DATLBZ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4858899"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..985
                     /locus_tag="VMZ53_17460"
     CDS             143..985
                     /locus_tag="VMZ53_17460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS30302.1"
                     /translation="MRCPICDASELDQHGHYGQCHACGYWQSDLDHDVTNTSAPDEEY
                     ELVSYEHTRRANYSRILQLLAKRHPAGSTMLEVGCADGLFLDMATRDNGYDVIGIEPN
                     TRMMAGNKLGHQIRQGFFPDVLAPDEKFDIIALNCVFEHVPDLAAMIGAFKRHLEPSG
                     SVMLNVPVASGFMFKAARQLYRLGVKYPFDRLWQKGFVSPHLHYFARKSLQQLLGKHG
                     FSLTFETPLAMFSLGGIYARLSLDPNIRFAQRIAALGSLYAYYPVSRVAPDARAFVFV
                     RGAG"
     gene            complement(922..1896)
                     /locus_tag="VMZ53_17465"
     CDS             complement(922..1896)
                     /locus_tag="VMZ53_17465"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUS30303.1"
                     /translation="MSLAGLDIAVLVPCFNEAAAIAKVVNDFKTSLPTARVYVYDNNS
                     TDETVPVAAAAGAEVRSETRRGKGNVVRRMFQDVDADVYIMVDGDDTYDAAVAPQLVE
                     QLVEDNLDMLVGKRVETHQAAYRMGHRLGNRVLTGLVAWLFGSQIDDMLSGYRVFSRR
                     FVKSFPSFSREFEIETELTVHALQMKMAIAEVDTNYKERPPGSVSKLRTFRDGWRILV
                     TIINLMRNERPLLFFGLLGMLFLGTAVGLALPVFLEYLDTGYVRRFPTAILSFSLGVV
                     AVISWATGLILDLVAHVRRETKKLAYLSYPAPRTKTNARASGATRETG"
     gene            complement(1817..2740)
                     /locus_tag="VMZ53_17470"
     CDS             complement(1817..2740)
                     /locus_tag="VMZ53_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30304.1"
                     /translation="MLGFLVLLLPAVANAKPARTKPPIDQLRFERHAIAATGDTRVES
                     RTIYLNPYGVVLTPGDNDSRTDASSIVTSPTVINGWDIDVDTWNETVDCMREIYAPFD
                     VTITDEDPGNVPHIEAVFGGSPTDVGLPDNVAGVSPFTTDCAVIENSIVFTFTDVIPD
                     DARTACEIMAQEVAHSFGLDHEMLASDPMTYLPYYGERTFQNEMASCGEFGDRMCGIN
                     GNVCRYQQNSVKLLGQRLGLKGAAPPAGEPTPDGEDDPDVASGVTGCDAGGGSGIALV
                     LLTLRLVFGRSRHRRPRALLQRSGSDCEGGQ"
     gene            complement(2887..4437)
                     /locus_tag="VMZ53_17475"
     CDS             complement(2887..4437)
                     /locus_tag="VMZ53_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30305.1"
                     /translation="MRPRLVPVIEIAVSFAAAALVAWLSTFIKVNPLDRVGQVSGLAA
                     IQIRYLACAIPVLVAVAVSTRYRDGRWFPITSRMACAALAGLASGFVAGGILVALRGT
                     PFCLNAGAGDSDILVMWANAYHRGDATQYPPAFYPPLFPQLLRYYAEATDQPAVYALK
                     DLQILFTATAGPVAYCAWRLILRPGWALGVGVVAAMVIIDPYKPYGAFVLVVFVPIVI
                     RYFARLREAGAKHPYELVRTGVIYGLVLGVLCLMYSGWFRWAGAGLVIAGCVVFPWRR
                     WKHGAVLVAVTLVVILIVDWSYITGLLGFAQKLQQAEQASGIPHPLIADEYIYFDSVA
                     EPTFFTLWKGDLPGTTVEADWPPLGELGGLGLFTIALFAGTGTALALGRRRTDIITMV
                     SVLGAIWVQRFWTARAMFKTKLVQLWPRTTLALIYLFVLLTGFAAYFIYERKLARAGE
                     DSPLRAPSSVAGALVALTLLFGSMGSSISNRYMPYSSLPRTTGWLAFLAHDAWQKVKA
                     APETAPKL"
     gene            4490..>5208
                     /locus_tag="VMZ53_17480"
     CDS             4490..>5208
                     /locus_tag="VMZ53_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30306.1"
                     /translation="MAVNVRELGQKLLGWLTTRRPRAYLFAGWILFVLGSYPGYMNSE
                     SILQLYTVRSGDYSDFSPMMTAVWSILEWVIAGPFGLLALQSALFLFGAWAILATLLR
                     PRVAAVTASLVLLFPPVFSVMAVIWPDPLMAASLLAATGAALQGTKRWIAAAFCFALL
                     ACACRPDAVVAVGAILLALVSAPSTLRRVAISLGASIVILLLALGANELATVKDTYVR
                     EHNLQMLDIVGTLRRAKIKDEA"
BASE COUNT          923 a         1712 c         1712 g          861 t
ORIGIN      
        1 gctgtcgctg ttgccgatcg cgatcacacc ggactaccgc tactcgcact ggctcgtcgt
       61 gacgacggtg ctctcggtcg tgatgctcgt cgcgaggcgc gcgcggccag cgagcaccaa
      121 cgccacgtga tataggaccg cggtgcgctg cccgatctgc gatgcgtccg agctggacca
      181 gcatggtcac tacgggcagt gccacgcgtg cggctattgg cagagcgatc tcgatcacga
      241 tgtgacgaac acatcggcgc ccgacgagga atacgagctc gtatcgtacg agcacacgcg
      301 gcgcgcgaac tactcgcgca ttctgcagct gctcgcgaag cggcatccag cgggcagcac
      361 gatgctcgag gtcggctgcg ccgacggctt gttcctcgac atggcgacgc gcgacaacgg
      421 ctacgacgtc atcggcatcg agcccaacac gcggatgatg gcgggcaaca agctcggtca
      481 tcagatccgg cagggcttct tcccggacgt gctcgcgccc gacgagaagt tcgacatcat
      541 cgcgctcaac tgcgtgttcg agcacgtgcc ggatctcgcc gccatgatcg gcgcgttcaa
      601 acgccacctc gagccgtcgg gcagcgtgat gctgaacgtg cccgtggcgt cgggcttcat
      661 gttcaaggcg gcgcgccagc tctaccggct cggcgtgaag tatccgttcg accggctgtg
      721 gcaaaagggc ttcgtcagcc ctcacctgca ctacttcgcg cgcaagagcc tgcagcagct
      781 gctcggcaag cacggcttct cgctgacgtt cgagacaccg ctcgcgatgt tctcgctcgg
      841 tggcatctac gcgaggctgt cgctcgatcc gaacatccgc ttcgcgcagc ggatcgccgc
      901 gctcggctcg ctgtacgcgt actaccccgt ctcgcgcgtc gcgccggatg cacgtgcatt
      961 cgtcttcgtg cgcggcgcgg gatagctgag atacgcgagc ttcttcgtct cgcggcgcac
     1021 gtgcgcgacg agatcgagga tgaggccggt cgcccacgag atcacggcga cgacgccgag
     1081 cgagaacgac aggatcgcgg tcgggaaccg acgcacgtag cctgtgtcga gatactcgag
     1141 gaagaccggc agcgcgaggc cgacggcagt gccgaggaac agcatgccga ggagcccgaa
     1201 gaacaacagc gggcgctcgt tgcgcatgag gttgatgatc gtgacgagga tgcgccagcc
     1261 atcgcggaac gtgcggagct tgctgacgct gcccggcggc cgctccttgt agttcgtgtc
     1321 gacctcggcg atcgccatct tcatctgcag cgcgtgcacg gtgagctcgg tctcgatctc
     1381 gaactcgcgc gagaacgacg ggaagctctt cacgaagcgg cgagagaaca cgcgatagcc
     1441 cgagagcatg tcgtcgatct gcgagccgaa cagccacgcg acgagtccgg tgagcacgcg
     1501 gttgccgagc cggtggccca tgcggtacgc ggcttgatgc gtctcgacgc gcttgccgac
     1561 gagcatgtcg aggttgtcct cgacgagctg ctcgactagc tggggcgcga ccgccgcgtc
     1621 ataggtgtcg tcgccatcga ccatgatgta gacgtcggcg tcgacgtctt ggaacattcg
     1681 acggacgaca ttacctttgc cccggcgtgt ctcggagcgc acctcggccc cggcagccgc
     1741 ggcgaccggc acggtttcat cggtcgagtt gttgtcgtag acgtaaaccc gagcggtagg
     1801 caggctcgtc ttgaaatcat tgaccacctt cgcaatcgct gccgcttcgt tgaagcaggg
     1861 cacgaggacg gcgatgtcga gaccggccaa agacaaggcg cagcgttaat aacacgagcg
     1921 cgattccgga gccgcctcct gcatcgcagc cggttacgcc cgaggcgaca tcgggatcgt
     1981 cctcgccgtc tggcgtgggc tccccggcag gcggcgccgc gcctttgagc ccgagccgct
     2041 ggccgagcag cttgaccgag ttctgttgat agcggcagac gttgccgttg atgccgcaca
     2101 tgcggtcgcc gaactcgcca cacgaggcca tctcgttctg gaacgtgcgc tcgccgtagt
     2161 acggcaagta cgtcatcgga tcggacgcga gcatctcgtg atcgagcccg aagctgtgcg
     2221 cgacctcttg cgccatgatc tcgcacgcgg tgcgcgcgtc atcggggatc acatccgtga
     2281 acgtgaacac gatcgagttc tcgatcaccg cgcagtccgt cgtgaacggc gacacgcccg
     2341 caacgttgtc gggcagcccg acgtccgtgg gtgagccacc gaagaccgcc tcgatgtgtg
     2401 gcacgttgcc gggatcctcg tcggtgatcg tcacgtcgaa cggcgcgtag atctcgcgca
     2461 tgcagtcgac cgtctcgttc caggtgtcga cgtcgatgtc ccagccgttg atgaccgtcg
     2521 gcgatgtgac gatcgacgag gcgtcggtac gcgagtcgtt atcgccgggc gtgagcacga
     2581 caccgtacgg gttgagatag atcgttcgac tctcgacgcg tgtgtcaccg gtcgcggcaa
     2641 tcgcgtggcg ctcgaagcgc agttgatcga ttggaggctt cgtccgcgcg ggcttcgcgt
     2701 tggctaccgc ggggagcaac agaacgagga aaccgagcat gcgttccatg ggacgcagct
     2761 ctgagcatct cccgtgccgc gatctccgcg tgagcgcagc agagttagcg cacgtggggc
     2821 tggcgtctcc agtcgcctgt gggggactgt gctcgccgtc gacgacggga gacagctgtc
     2881 acttcgctac agcttcggcg ccgtttcggg cgcggccttt accttctgcc acgcgtcgtg
     2941 cgcgaggaac gcgagccacc cggtcgtgcg cggcagcgag ctgtacggca tgtagcgatt
     3001 gctgatcgag ctgcccatcg agccgaagag cagcgtcaac gcgacgagcg cgccggcaac
     3061 cgagctcggt gcgcgcagtg ggctgtcctc gcccgcacgc gcgagcttgc gctcgtagat
     3121 gaaatacgcc gcgaagccgg tgagcaagac gaacaagtag atgagcgcga gcgtggtgcg
     3181 aggccacagc tggacgagct tggtcttgaa catcgcgcgc gcggtccaga accgctgcac
     3241 ccaaatcgcg ccgaggacgg agaccatcgt gatgatgtcg gtgcggcgac gcccgagcgc
     3301 gagtgcggtg ccggttcccg cgaacaacgc gatcgtgaac aggcccagcc cgccgagctc
     3361 gccgagcgga ggccagtcgg cctcgacggt cgtgccaggc aagtcacctt tccacagcgt
     3421 gaagaacgtc ggctcggcga cggagtcgaa gtagatgtac tcgtcggcga tgagcgggtg
     3481 cgggatgccg ctcgcttgct cggcctgctg gagcttctgc gcgaagccga gcagcccggt
     3541 gatgtagctc cagtcgacga tcaagatcac gacgagcgtg accgcgacga gcacggcgcc
     3601 gtgcttccaa cgtcgccacg gaaagacgac gcagccggcg atgacgagcc ctgcccccgc
     3661 ccaccggaac cagccggagt acatgaggca cagaacgccg aggacgaggc cgtagatcac
     3721 gccggtgcga acgagctcgt acggatgctt cgctccagct tcacgcagcc gtgcgaagta
     3781 gcgaatgacg atcggtacga acacgacgag gacgaacgcg ccatacggct tgtacggatc
     3841 gatgatgacc atcgccgcga ccacgccgac gccgagcgcc cagcccggcc gcaggatgag
     3901 tcgccacgcg caatacgcca ccgggcccgc ggtcgcggtg aagaggatct gcagatcctt
     3961 gagcgcatac accgccggct gatccgtagc ttccgcgtag tagcgaagca gctgcggaaa
     4021 cagcggtgga tagaacgccg gcggatactg cgtcgcatca ccgcggtgat acgcgttcgc
     4081 ccacatcacg aggatgtccg aatcgcccgc gcccgcgttg agacagaacg gagtgccgcg
     4141 cagcgcgacg aggatgccgc ccgcaacgaa gcctgatgcg agccccgcga gcgccgcaca
     4201 cgccatccgc gacgtgatcg gaaaccatcg cccatcgcga tagcgcgtcg acaccgcgac
     4261 cgcgacgagc acgggaatcg cgcacgcgag ataccggatc tggatcgcgg caagaccgct
     4321 gacctggccg acccgatcga gagggttgac cttgatgaac gtggacagcc acgccaccaa
     4381 ggccgccgcc gcgaacgaga ccgcgatctc gatcacgggc acgagccgag gacgcatccg
     4441 cggccagctt atcgcatctt gatcgccttc gtgatatcgc ggccatgcag tggcggtgaa
     4501 tgtccgcgag ctagggcaaa aactcctcgg gtggctgacg acgcgccgcc cgcgcgccta
     4561 tctgttcgcc ggctggatcc tgttcgttct cgggtcgtac ccgggctaca tgaacagcga
     4621 gtcgatcctc cagctctaca ccgtccgcag cggcgactac tccgacttct cgccgatgat
     4681 gaccgcggtc tggtcgatcc tcgagtgggt gatcgcaggc ccgttcggtc tactcgcact
     4741 gcagagcgcc ctattcctat tcggcgcgtg ggcgatcctc gcgacgctgc tgcggccgcg
     4801 tgtcgccgcg gtcaccgcgt cgctcgtcct cctcttcccg cccgtcttct cggtcatggc
     4861 cgtcatctgg cccgatccgc tgatggctgc ctcgctgctc gccgcgacgg gcgccgccct
     4921 gcaaggcacg aagcgttgga tcgccgccgc attctgcttc gcgctgctcg catgcgcatg
     4981 ccgtcccgac gccgtcgtcg ccgtgggcgc gattttgctc gcgctcgtgt cggcgccatc
     5041 gacgctgcgc agagtcgcga tcagcctcgg cgcgtcgatc gtgatcctgc tgctcgcgct
     5101 cggcgcgaac gagctcgcca ccgtcaaaga cacgtacgtg cgcgagcaca acctgcagat
     5161 gctcgacatc gtcgggacgc tgcgccgcgc gaagatcaaa gacgaggc
//
LOCUS       DATLBZ010000338         4784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3188549, whole genome shotgun sequence.
ACCESSION   DATLBZ010000338 DATLBZ010000000
VERSION     DATLBZ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188549"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VMZ53_17485"
     CDS             <1..475
                     /locus_tag="VMZ53_17485"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HUS30307.1"
                     /translation="WRTQISMFDVSDFAHPTLQEALPVEAQNTWGWSEALYDHHAFQY
                     WAPKKLLAIPQSTYAYQNWNGSYYYKYLSQLVLVNVDDTAGLSIHGRIDHSSFYNADP
                     NNYWQWTDIRRSIFMGDYVYAISDKAVTAHRVSDLGQTAAQLLPGYQPGQYYWWW"
     gene            593..1033
                     /locus_tag="VMZ53_17490"
     CDS             593..1033
                     /locus_tag="VMZ53_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30308.1"
                     /translation="MKRSLLLTALTIALLGGTAFADRHHHRGGWGRSHHGSWRVNAGV
                     VVQPRVVVQQPRVVVVQQPRVVQRRVVVQQVQPVVVQAPANSYWVEGQWQWTGYQWQW
                     QAGHYETYQPQPAYEQGYYDQPAYNDNYVEGSFEAGWSSQPCDH"
     gene            1145..1585
                     /locus_tag="VMZ53_17495"
     CDS             1145..1585
                     /locus_tag="VMZ53_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30309.1"
                     /translation="MMQLILALVIGGAIVLFASLLRAAAGPRRRTRRRADSFTMDDSF
                     ALHAALMQSDPTQHTAVHHGATHSGDDISTSHSHSHSHSHCDTGSGYSHSHSHSDYSS
                     SHSHCDVGSSGGSDFGGGSAGSDFGGGGSSDFGGGGDFGGGHHH"
     gene            complement(1613..3370)
                     /locus_tag="VMZ53_17500"
     CDS             complement(1613..3370)
                     /locus_tag="VMZ53_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30310.1"
                     /translation="MRRWVLVAALAACGDKGAAPDARLEGFDRPDIVCPGDPSCKSAG
                     DGQLKVGVGKRIWTPQNFETYTDENQDREWQKTEPFTDLNGNGKFDGVWLFGGGRAAL
                     GVTTDVEVRAMSFIEGDMTFVIAYVDCVGMFSGDMEAIRSDPALAGLNIDHIMIGSTH
                     AHDAPDTAGIWGPDVGVSGRQQFVIDVLKAKAVEAIAEAVQSAQPAQMIIASTKTLNE
                     LGNTQSRTDDWFKDIRDPVIFDPTVTIARFVKVSNPNETIGTLVNWADHPEVAHFDDT
                     VPASITAHYPHWLRDHIENGVTTAESKFATTDLPGLGGVTVFVQGALGGQIGSLRGTH
                     PPGPGGVQITKTSHAMDQAVGTNLAAVALTTLAQSGESTSELPLSFKSAKFWARVDNL
                     GIQVYQILGILGPTEVGGYNPEDPIDVGNEPWISLRSVYLQIGPFGLVTVPGELHPEL
                     WVGGYNGDWSFGWPIQWGECADNGNQCELGTNAGCAASVICNAKPNAPDLSTAPKPPY
                     MRDLVLAHEGVRYPAVAGLAENYIGYIVPMYNFVLATSGAWLNEAEGDHYEEVYALST
                     LVEQHVIHPVLELLKYRKK"
     gene            3406..>4784
                     /locus_tag="VMZ53_17505"
     CDS             3406..>4784
                     /locus_tag="VMZ53_17505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HUS30311.1"
                     /translation="MVAKTVMAEPRAVLREVYGYDEFRAGQADAVDAVLDGRDAVVLL
                     PTGAGKSVCYQVPAITLARAGRGTTIVVSPLIALMIDQVDALVGRGIAAAAVHSHQEE
                     DEQRAVVGRFVRGELALLYVSPERAALDSFKRLLTRTPIALFAIDEAHCVSQWGHDFR
                     PEYMRLAELREVAGRGDVPLIALTATATPRVMAEITKSLGLRAPTQVRGDFRRPNLTF
                     SVRHASRDEDRLAATVAALDAAGLRGRTSTGRGIVYCSTRKKTESVAQELKSLGFAAG
                     HYHAGRTPLARDRMHNAFSAGRTRVLVATSAFGMGVDYPDVRAIVHFQAPGSLEAYYQ
                     EAGRAGRDGLPATCLLLFGAADLMTQRRLGERGRAIDTRLEDALAAIERYSRARTCRQ
                     QMLCAHFTGHDDHAACNLCDASTNADELELEPVAREPAELLSSTDQQLIIEAVGALSR
                     AIGKTNLAKA"
BASE COUNT          794 a         1553 c         1594 g          843 t
ORIGIN      
        1 ctggcgcacg caaatctcga tgttcgacgt cagcgacttc gcgcacccga cgctgcaaga
       61 ggcgctgccg gtcgaagcgc aaaacacatg gggctggtcg gaagcgctct acgatcacca
      121 cgcgttccaa tactgggcgc cgaagaagct gctcgcgatt ccgcagtcga cgtacgccta
      181 ccagaactgg aacggcagct actactacaa gtacctctcg cagctcgtcc tcgtgaacgt
      241 cgacgacacc gcgggtctct cgatccacgg ccgcatcgat cactcgagct tctacaacgc
      301 cgacccgaac aactactggc agtggacgga catccgtcgc tcgatcttca tgggcgacta
      361 cgtctacgcg atcagcgaca aggccgtcac cgcgcaccgc gtgagcgacc tcggccagac
      421 cgccgcgcag ctgctccccg gttatcagcc cggccagtac tactggtggt ggtgagcccc
      481 gcaccgcgcg ccgtccgcgt aaacgcgcgg tcacaccgct cgcagctcgc ctccgcaaat
      541 cgctgaaaat cgctgcccgc cggttcggca cacatgatgc tgaacggcgg gcatgaagcg
      601 ttctctactt ctgacagctc tgacaatcgc gctgctcggt ggcaccgcct tcgcagatcg
      661 tcatcatcat cgcggcggct gggggcgcag ccatcacggc agctggcgcg tgaatgcagg
      721 agtggtcgtg cagccacggg tcgtcgtgca gcagccgcgc gtcgtcgtcg tgcagcagcc
      781 gcgcgtcgtg cagcgtcgcg tcgtcgtgca gcaggttcag ccggtcgtcg tgcaggcgcc
      841 cgcgaattcg tactgggtcg aaggccagtg gcagtggacc ggttaccaat ggcagtggca
      901 agcgggccac tacgagacgt atcagccgca gcccgcgtac gagcagggct actacgatca
      961 gcccgcgtac aacgacaact atgtcgaggg ctcgttcgaa gcaggatggt cgtcgcagcc
     1021 gtgcgatcac taacttgcag cgactcccgt ctgtgacggg acggtgacgg gaccgcaaac
     1081 tcgcagctgg gtcgattgcg cccgctttct gccgtgagca cgtttgaggc aaagggggtc
     1141 cgaaatgatg cagctgatcc tggcgctcgt catcggcggt gcaatcgtcc tgttcgcgtc
     1201 cttgctccgt gctgcggcgg gaccgcgccg ccggacgcgt cgacgagccg attcgtttac
     1261 gatggatgat tcgttcgcgc ttcacgcagc gctgatgcag tccgacccga cgcagcacac
     1321 cgccgttcat cacggcgcga cgcacagcgg cgacgacatc agcacgagcc acagtcacag
     1381 ccacagccac agccattgcg acaccggcag cggctatagc cacagtcaca gccacagcga
     1441 ttacagcagc agccacagcc attgcgatgt gggttcgagc ggcggcagcg acttcggcgg
     1501 cggcagcgcc ggcagcgact tcggcggcgg cggcagcagc gacttcggtg gcggtggcga
     1561 cttcggcggc ggccaccatc actagcgcga tcctggagcc gcgagagcgc cgctacttct
     1621 tgcggtactt cagcagctcg agcaccggat ggatcacgtg ttgctcgacg agcgtcgaga
     1681 gcgcgtagac ctcttcgtag tgatcgcctt cagcttcgtt gagccacgcg ccgctggtcg
     1741 cgagcacgaa gttgtacata ggcacgatgt agccgatgta gttttcggcg aggcccgcga
     1801 cggcggggta gcgaacgcct tcgtgagcga gcacgaggtc gcgcatgtat ggcggctttg
     1861 gggccgtgga cagatcgggc gcgttcggct tcgcgttgca gatcacgctc gcagcgcagc
     1921 ccgcgttcgt gccgagctcg cattggttgc cgttgtcggc gcactcgccc cattggatcg
     1981 gccagccgaa cgaccagtcg ccgttgtagc cgccgaccca gagctcgggg tgcagctcgc
     2041 cgggaacggt gacgagaccg aacggaccga tttggaggta cacgctacgc agcgagatcc
     2101 acggctcgtt gccgacgtcg atcggatctt cggggttgta gccgccgact tccgtcgggc
     2161 cgaggatgcc gaggatttgg tagacctgga tgccgaggtt gtcgacgcgc gcccagaact
     2221 tggcgctctt gaagctgagc ggcagctcgc tcgtcgattc gccgctctgt gcaagcgtgg
     2281 tgagcgcgac cgcagcgagg ttcgtgccaa ccgcttgatc cattgcgtgc gacgtcttcg
     2341 tgatctgcac gccgccggga ccaggcggat gcgtgccgcg cagcgagccg atttggccgc
     2401 cgagcgcgcc ttgcacgaag acggtgacgc cgccgaggcc cggcagatcc gtcgtcgcga
     2461 acttgctctc ggccgtcgtg acgccgttct cgatgtgatc gcgcagccag tgcggatagt
     2521 gcgcggtgat cgatgccggg acagtgtcgt cgaagtgggc gacctcgggg tggtccgccc
     2581 agttgacgag cgtgccgatc gtttcgttcg ggttcgagac tttgacgaag cgcgcgatcg
     2641 tgacggtggg atcgaagatg actggatcgc ggatgtcctt gaaccaatcg tcggtgcggc
     2701 tctgcgtgtt gccgagctcg ttgagcgtct tcgtagacgc gatgatcatt tgcgcgggct
     2761 gcgccgattg cacggcttct gcgatcgcct cgacggcctt cgccttgagc acgtcgatca
     2821 cgaattgttg gcggccgctg acgccgacgt cgggtcccca gatgccggcg gtgtcgggcg
     2881 cgtcgtgcgc gtgcgtcgag ccgatcatga tgtgatcgat gttgaggccg gccagcgctg
     2941 ggtcgctgcg gatcgcctcc atgtcgccgc tgaacatgcc gacgcaatcg acgtacgcga
     3001 tgacgaacgt catgtcgcct tcgatgaagg acatcgcgcg gacttcgacg tcggtcgtga
     3061 cgccgagcgc tgcgcggcca ccgccgaata gccacacgcc gtcgaacttg ccgttgccgt
     3121 ttagatcggt gaatggctcg gtcttctgcc attcgcggtc ttggttttcg tcggtgtacg
     3181 tctcgaagtt ctgcggcgtc cagatgcgct tgccaacgcc tactttgagc tggccgtcgc
     3241 ctgcgctttt gcagctcgga tcgccggggc agacgatgtc gggccggtcg aagccctcga
     3301 ggcgagcatc gggggccgcg cctttgtcgc cgcacgcagc tagtgcagcg acgagaaccc
     3361 atctcctcat catgggccgg gtcgtaccac gaacgtcata ccggcgtggt agccaaaacg
     3421 gtgatggccg aaccgcgtgc ggtacttcgc gaggtctacg gctacgacga gtttcgggcc
     3481 gggcaggccg acgctgtaga cgccgtcttg gacgggcgcg acgcagtggt gttgttgccg
     3541 acgggcgccg gtaagtcggt ctgttatcaa gtgcccgcga tcacattggc gcgggcggga
     3601 cgcgggacga cgatcgtcgt gtccccgctc atcgcgctga tgatcgatca ggtcgatgcg
     3661 ctcgtcgggc gcgggatcgc ggcagcggcg gtgcacagcc accaggagga ggacgagcag
     3721 cgtgcggttg tcgggcggtt cgtccgcggc gagctcgccc tgctctacgt ctcgccggaa
     3781 cgcgcagcgc tcgacagctt caagcgcctg ctgactcgca cgccgatcgc gctctttgcg
     3841 atcgacgaag cgcactgcgt gagccaatgg ggccacgatt tccggcccga gtacatgcgc
     3901 cttgccgagc tgcgcgaagt cgccggtcgc ggcgatgttc cgctgatcgc gctgaccgcc
     3961 accgcaacgc cgcgcgtgat ggcggagatc acgaagtcgc tcgggcttcg cgcgcccacg
     4021 caggtgcgcg gggattttcg ccggccgaac ctgacgttct ccgtccgcca tgcgtcgcgc
     4081 gacgaagatc gactcgccgc gacggtagcg gcgctcgatg cagcaggcct gcgcggtcga
     4141 acgtcgacag gtcgcggcat cgtttactgc agcacgcgca agaagacaga atcggtcgcg
     4201 caggagctga agtcgctcgg cttcgccgcg ggtcactatc acgcgggccg cacgccactc
     4261 gcgcgcgatc gcatgcacaa cgcattctcc gcagggcgca cgcgcgtgct cgtcgcgacg
     4321 agtgcattcg gcatgggcgt cgactatccc gacgttcgcg cgatcgttca cttccaagcg
     4381 ccgggctcgc tcgaggcgta ctaccaagaa gccgggcgtg cgggccgcga tggtctcccg
     4441 gcgacgtgtc tcctcctctt cggcgccgcc gatctaatga cgcaacgccg gctcggcgag
     4501 cgcggccgcg cgatcgacac gcgcctcgaa gatgcactcg ctgcgatcga acgctactcg
     4561 cgtgcgcgca cgtgtcgcca gcagatgctg tgcgcgcact tcacgggcca cgacgaccat
     4621 gccgcatgca atctctgcga cgccagcacg aatgccgacg agctcgagct cgagcccgtc
     4681 gcgcgcgaac cggcggagct gctgtcgtcg acggatcaac agctgatcat cgaggcggtc
     4741 ggcgcgctgt ctcgcgcgat cggcaagacg aacctcgcaa aggc
//
LOCUS       DATLBZ010000339         8648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3188660, whole genome shotgun sequence.
ACCESSION   DATLBZ010000339 DATLBZ010000000
VERSION     DATLBZ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188660"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VMZ53_17510"
     CDS             complement(<1..450)
                     /locus_tag="VMZ53_17510"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HUS30312.1"
                     /translation="MLETLLDYLREPLVVLSGTQISALTLLTAILIIIVAKIASVVIG
                     RSIERVLEVRDVDKGVRFAAGRIARYVVIVIGMFVAIGTLGIDTSAMMGAAAVLLVGI
                     GFGLQKLAENFISGLLVLIERPVRKGDFIDAGGVLGVVDDIGLRATRV"
     gene            complement(474..5465)
                     /locus_tag="VMZ53_17515"
     CDS             complement(474..5465)
                     /locus_tag="VMZ53_17515"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HUS30313.1"
                     /translation="MPARKITLRSIFRWTRRIVLGSIALVILLAAIAIGIVHTKFGRE
                     RIRKELESALQDDFPGSTVGRVDGSPFGSLTATGIAIAGKDGKPFITIESAELGVQLR
                     PLLRKTVHVDTVTLHNVLVDLAAQPDATSKTPDPMKPQPATTTNDKPSSWDIEVPSIV
                     VRDARVLTAEGERLDGIDLDAGIRLHDGTLSALASLRAQFRGRPFMAAASIHYGAPAL
                     AASIAANAAAGNDTSTTTAGKTLDVPYAHVTLGDADITLAGAHIAVEGALVVDGTLIA
                     RVPAALVNELAKLPPESQIPGDIALIVTNKAGNISFDASIAPTDLTGLLPAGLAPAGL
                     AAAGLTPAALAPAPVSDPRTPSDPRTPSDKAASDKATATSDPRTPSDKADDGRTPIAN
                     AVVASATARSLSELRTRVRGFAHIDLSKLDFAKTDFSKLDSVGDATGELLVAVDDPRG
                     RATFALSGSTAGVRGLIAAEGAYALAMDDGARLDLTGGALIALDVKRDGDAWNLVSAL
                     VSSDSAGKLAELAVTTKKRAPAYTYTASGTAATSANVHGTLWPVLALDAEGVLDARGL
                     RYDETRVEKAHMHFDAKNVTGTPVIASRVEVEHVQQGDRVVAVMIPSAAVAATTTLHE
                     DGRIDVELGEHRLVTADGKTWKGRGGHVAVTAKTIDVRELQTGTGSSTVTASAIVGRL
                     NDDISGKAHVKNVSLAMIDPTLRGIVGGELTFARKAGRYSGHGAFDGKGIVVPSTTGT
                     PGTTGTPGTTGTPGTTGTPGTTGTPGTSGTPGTSGTPGKPAPRPGQPGYHAYPAIDGH
                     VAIELAGRRVHVEGNASTTAGSARLAVDLEGPRDITDPVAWKMLERSALHELTIGIEH
                     IDASKLGLTDANDPTATPVTGIVDGELVLTADGASGSVRVNGITTKAGTVETTLTIKP
                     GTTTSKIPSSSNPANGGNAANIANAANAANAANIANAANAGKAADIANAANVDARATA
                     TKTIDVHVDASLAGVKPATVDATVALPAHPFDPDAWALLGKGALKHATIRADHVPFDR
                     ALIARLGGDRDLSGVVDATVEVESALSAATVVVDVTNVKGPELRAPVSLHAQGGIGGN
                     GATGSASAKMASVAITLDAKSPLTMTGAMAGGAAAAGLDATLTIPTTKATEILALLGR
                     QDLTDGTVAGVVKVGGTVDKPNVVANLTASNLTPPPTLATKKPAKLEKLDVSARWTGT
                     HAELEVTGFETGGKLLKISGRGDIGKPASVAATIEAAGFDIAPIVAFAPGALGAARGT
                     LDGVIKVNGLDPQTGDVRGKLHLTDGRLPLTPTLGTVRKADIEISIAKQDVKAVISAK
                     LGAGSITGKASARLAGGMPQVADFDLALRKLSPIGAIQPVVDADAWGKFEIADGKVTG
                     KINVRKGKVYVPPEEGDELIDTGAPADMFFVGADGRIVPKPVRKQAEKPWLFAEIEIG
                     TTAVTVDDPNFYFDGRASGLLHLKVGDGVTLEGSLATERGAVDVLNRRYQIDHAILEF
                     EGTTDPRLDIRLIHDFRNLTLTVDILGTSLIPDVRLKGDPANYTQGQLFAFLAGAEPG
                     DDSSGAAGQAVTGAASALLSSRLGRKVNKYLPVKLDTLNYEAGTASSSGAVRAGVRLS
                     EKSHLLWRQRLEARPDENTGEAVFEYIIKPWALVEATGGPRAGGGDILLRKRW"
     gene            complement(5469..7472)
                     /locus_tag="VMZ53_17520"
     CDS             complement(5469..7472)
                     /locus_tag="VMZ53_17520"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HUS30314.1"
                     /translation="MSGSRSVALTAVVAIVAACGGGQKTVRRPGEEYLAEIRIEANKK
                     ALDFNPKNIIPGLALNRAKGKRALDEYQLNLDVTRIEGTFQKHGYFAAKVTPRVERKG
                     DKQIAIFHVDEGPQAKAKVDIIGLPPEVPLAEARALVPVKDGAAFSYDAFDGAKTPML
                     ALVENAGYAHAVMQAAVLADKAANTATLQYAFDPGPRTTFGAIEIVGVDGVLADAARA
                     RLTFKPGDGYSTKALAQSQEAIYGIGMFSAVRVDADRTTLDASVPVRIQLTEALHWEA
                     RGGIGAGIDILSYNVRLRGSLSHTGWPTPLSTLSLEARPALVVLRDHCPVLGLVGFDE
                     CKMSDSDDSFPLEPRIRVLGSAVQQDFLRPGVKGELQGGYDYVTLEGFVEYGPLVRLG
                     ISSPLGTPRLQGHLGWQFANYTFGNLVPPLDPTPDAGATEVAYDPTRAESLGLLEDER
                     LGAFTQALELDLRDNPVEPTVGGYAQIKVSEGTVAAGGAFNYLQLIPELRGYYSLGGA
                     VFAAHARFGAILGDVPPTERFYAGGAASQRGFADRRLSPEVIAVERDPATGVVGTNIT
                     SSVVVGGAGLFETGIEVRRHWEPLGIKAGWVVFLDGGDVTSKPADLDLTHLHWAVGAG
                     LRFFYLPIGPIRLEVARRINRTGGNEPSRGQLFNFILSVGEAF"
     gene            7492..>8648
                     /locus_tag="VMZ53_17525"
     CDS             7492..>8648
                     /locus_tag="VMZ53_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30315.1"
                     /translation="MLGMKTLLPLVLFTATATAKGLTIEDMLAMQRVSDPQVSPDGKW
                     VAFSVRDTDYDANKGRFDVYVAAVDGSSVTRLTTDEANDTDAKWSTDGKWLYFMSTRS
                     GSGQVWRIRPTGGEAEQVTKLDTDINAFRVMPDGKRLVLAIDVWPDAKTLADSIKRDE
                     AKDKSKVKARVYDQLLFRHWDTWEDGKFSHLFMWSSDSDVKDLTAGLSTDTPIHPFGG
                     SEQFTVSPDGKLLAYVARVGGKEIAYTTNTDIFLTSTDGKGKAANITGDNKAYDFDPV
                     WSRDGKSIAFTAMKKPGFEADRTRITIYDVASKKQRVVTEGWDRSAGTLTWSADGKTI
                     YTNAENVGNEAIFAIDVTNGTAKPLVEKGSNNSAQLAGDRLVFSKDTLKMPA"
BASE COUNT         1357 a         2872 c         2936 g         1483 t
ORIGIN      
        1 gacgcgagta gcgcggaggc cgatatcgtc gacgacgccg agcacgccgc ccgcatcgat
       61 gaagtcgccc ttgcgcacgg gacgctcgat cagaacgagc aagccggaga tgaagttctc
      121 ggcgagcttc tggagaccga agccgatacc gacgagcagg acagcggccg cgcccatcat
      181 cgcgctcgtg tcgatgccga gcgtgccgat cgcgacgaac atgccgatga cgatgacgac
      241 gtagcgcgcg atgcgacccg cggcgaagcg aacgcccttg tcgacgtcgc gaacctcgag
      301 gactcgctcg atgctgcggc cgatcacgac cgacgcgatc ttcgcgacga tgatgatcaa
      361 gatcgcggtg aggagcgtca gcgcgctgat ctgcgtgccc gacaacacga cgagcggttc
      421 gcgcaggtag tccaggaggg tttcgagcat cgccttcgcg cgaaggtact atttcaccag
      481 cgcttgcgca gcaggatgtc cccgccgccg gcgcgtggac caccggtggc ttcgaccagg
      541 gcccacggct tgatgatgta ctcgaatacg gcctcgccgg tgttctcgtc gggacgcgcc
      601 tcgagccgct gcctccagag cagatgcgac ttctcggata gccggacgcc cgcgcgcacg
      661 gcgccgctcg acgatgccgt cccggcttcg tagttgaggg tgtcgagctt gaccggcagg
      721 tacttgttca ccttgcggcc gagcctcgac gacagcagtg ccgacgccgc gccggtgacc
      781 gcctggccag ccgcgccgct cgagtcgtcg ccgggctccg cgcccgcgag gaacgcgaac
      841 agctgcccct gcgtgtagtt cgccggatcg cccttcaggc gaacgtcggg aatgagcgac
      901 gtgccgagga tgtccacggt gagcgttagg tttctgaaat cgtgaatgag ccggatgtcg
      961 aggcgcggat cggtcgtgcc ctcgaactcc aggatcgcgt gatcgatctg atagcggcgg
     1021 ttcagcacgt cgaccgcgcc gcgctctgtc gcgagtgagc cctcgagcgt gacgccgtcg
     1081 ccgaccttca agtgcagcaa gccgctcgcg cgaccatcga agtagaagtt cggatcgtcg
     1141 accgtcacgg cggtcgtgcc gatctcgatc tcggcaaaca gccacggctt ctccgcctgt
     1201 ttgcgcaccg gcttcggcac gattctgccg tcggcaccga cgaagaacat atcggcgggc
     1261 gcgcccgtat cgatcagctc gtcgccttcc tcgggcggca cgtacacctt gcccttgcgg
     1321 acattgatct tgccggtgac cttgccgtcg gcgatctcga acttgcccca cgcatcggcg
     1381 tcgacgaccg gctgaatcgc cccgatgggc gacagcttgc gcagcgcgag gtcgaagtct
     1441 gccacctgcg gcattccgcc cgcgagtcgc gcgctcgcct tgccggtaat cgatccggcg
     1501 ccgagcttgg ccgatatcac agccttcacg tcctgcttcg cgatcgagat ctcgatgtcc
     1561 gctttccgga cagtgccgag cgtcggggtc agcggcagtc ggccatcggt caaatgcagc
     1621 ttgcctctca cgtcgcccgt ctgcggatcg aggccattga ccttgatgac gccatccaag
     1681 gtgccgcgcg ctgcgccgag cgcgccaggc gcgaacgcga cgatgggcgc gatatcgaag
     1741 cccgctgcct cgatcgtcgc cgcgacgctc gccggcttcc cgatgtcgcc gcgccccgaa
     1801 atcttcagca gcttgcctcc cgtctcgaag ccggtcacct cgagctccgc gtgcgtgccc
     1861 gtccaccgcg ccgagacgtc cagcttctcg agcttcgccg gcttcttcgt cgccagcgtc
     1921 ggcggcggcg tgaggttgct cgcggtcaga ttcgctacga cgttcggctt gtcgacggta
     1981 ccgccgactt tgacgacgcc agcaacggtg ccgtccgtca ggtcctgccg ccctagcaac
     2041 gccaaaatct ccgtcgcctt cgtcgtcgga atcgtgagcg tcgcatcgag gcccgccgcc
     2101 gccgcgccgc cggccatcgc ccccgtcatc gtcaacggtg acttcgcgtc gagcgtgatc
     2161 gcgacgctgg ccatcttcgc gctcgcgctg ccggtcgcgc catttccgcc gatgccgccc
     2221 tgcgcatgca gcgacaccgg tgcgcgcagc tcggggccct tcacgttcgt cacgtcgacc
     2281 accaccgtcg ccgccgacaa cgcgctctcg acctcgaccg tcgcgtcgac cacgccgctg
     2341 agatcgcgat cgccgccgag tcgcgcgatg agagcccggt caaacggcac gtggtccgcg
     2401 cgaatcgtcg cgtgcttcag cgcgcccttg ccaagcaatg cccacgcgtc cggatcgaac
     2461 gggtgcgcgg gcaatgcgac ggtcgcgtcc accgtcgcgg gcttcactcc cgcaagcgat
     2521 gcatcgacgt gcacgtcgat tgtcttcgtc gccgtcgcgc gcgcgtccac gttcgcggcg
     2581 ttcgcgatgt ccgcggcctt cccagcgttc gcggcgttcg cgatgttcgc ggcgttcgcg
     2641 gcgttcgcgg cgttcgcgat gttcgcggcg ttcccaccgt tggcggggtt cgatgagctc
     2701 gggatcttcg aggtggtcgt tccgggcttg atcgtgagcg tcgtctcgac ggtgccggcc
     2761 tttgtcgtga tgccgttgac gcggacgctg ccgctcgcgc cgtccgcggt caacacgagc
     2821 tctccgtcga cgatgcccgt caccggtgtc gccgtcgggt cgttcgcgtc ggtcaggccg
     2881 agcttgctcg cgtcgatatg ctcgatgccg atcgtcagct cgtgcagcgc gctgcgctcg
     2941 agcatcttcc acgcgaccgg atccgtgatg tcgcgcggcc cttcgaggtc gactgcgagg
     3001 cgcgcgctgc ccgccgtcgt cgacgcgttc ccctcgacgt gcacgcgccg tcccgcgagc
     3061 tcgatcgcta catggccgtc gatcgcggga tacgcgtgat agccaggctg gcctggccgc
     3121 ggtgcgggct tgccaggcgt gccgctcgtg ccgggtgtgc cgctcgtgcc gggtgtgccg
     3181 gtcgtgccgg gtgtgccggt cgtgccgggc gtgccggtcg tgccgggtgt gccggtcgtg
     3241 ccgggtgtgc cggtcgtcga cggcacgacg atgcccttac cgtcgaacgc gccgtgcccc
     3301 gaataccgcc cggccttccg tgcaaaggtc agctcgccgc cgacgattcc ccgcaacgtc
     3361 ggatcgatca tcgcgagcga gacattcttc acgtgcgcct tgcccgagat gtcgtcgttc
     3421 aaccggccga cgatcgcgct cgcggtcacg gtgctgctgc cggtgccggt ctgaagctcg
     3481 cgcacgtcga tcgtcttcgc ggtgacggcg acgtgcccac cgcgtccctt ccacgtcttg
     3541 ccatcggccg tcacgaggcg gtgctcgccc agctcgacgt cgatgcgacc gtcttcgtgc
     3601 agcgttgtcg tcgcggcgac cgccgcgctc ggaatcatca ccgccacaac tcggtcaccc
     3661 tgctgcacgt gctcgacctc gacccgcgac gcgatcaccg gcgtgccggt cacgttcttc
     3721 gcgtcgaagt gcatatgcgc cttctccacg cgggtctcgt cgtagcgaag gccgcgcgca
     3781 tcgaggacgc cttccgcatc gagcgccaac acgggccaca acgtgccgtg cacgttcgcg
     3841 ctcgtggccg ctgttcccga cgcggtgtac gtataagcgg gcgcgcgctt cttcgtggtc
     3901 acagcgagct cggcgagctt tcccgcgctg tcgctcgata cgagcgccga cacgaggttc
     3961 cacgcgtcgc catcgcgctt cacatccagc gcgatcagcg cgccgcccgt gagatcgagc
     4021 cgcgcgccat catccatcgc cagcgcgtat gcgccctccg ccgcgatcaa cccgcgtacg
     4081 cccgcggtcg accctgacag cgcgaacgtc gcacgcccac gtgggtcgtc gacggcgacc
     4141 agcaactcgc ccgtcgcgtc ccccacgctg tcgagcttcg agaagtcggt cttcgcgaaa
     4201 tcgagcttcg aaagatcgat atgcgcgaag ccgcgcacgc gtgtccgcag ctcggacaag
     4261 ctacgcgcgg tggcactcgc gacaaccgcg ttggcaatcg gcgtccgccc atcgtccgct
     4321 ttgtccgacg gtgtccgtgg gtcggacgtg gccgtcgcct tgtcggacgc tgccttgtcc
     4381 gacggtgtcc gcgggtcgga cggtgtccgt gggtcggaca cgggcgccgg ggcgagggcg
     4441 gccggggtga ggccggcggc ggcgaggccc gcgggggcga ggccggcggg gaggaggccg
     4501 gtgagatcgg tcggcgcgat cgatgcgtcg aaggagatgt tgcccgcttt gttcgtgacg
     4561 atgagcgcga tgtcgccagg gatttgggac tcgggcggca gtttcgcgag ctcgttgacg
     4621 agcgccgcag gcacgcgggc gatgagtgtt ccgtcgacga cgagcgcgcc ttcgaccgcg
     4681 atgtgcgcgc cggcgagcgt gatatcggcg tcgccgagcg tgacgtgcgc gtagggaacg
     4741 tcgagcgttt tgcccgctgt ggtggtgctg gtgtcgttgc ccgcggcggc gttggccgcg
     4801 atcgacgcgg cgagcgcggg ggcgccgtag tggatggagg ccgcggccat gaacgggcga
     4861 ccgcggaatt gcgcgcgcag tgaggcgagc gcggacagcg tgccgtcgtg caggcggatg
     4921 ccggcgtcga ggtcgatgcc atcgaggcgt tcgccttccg cggtgaggac gcgcgcgtcg
     4981 cgcacgacga tcgacgggac ctcgatgtcc cacgacgatg gcttgtcgtt cgtcgtggtg
     5041 gcgggttgcg gcttcatcgg gtcgggcgtc ttcgacgtgg cgtcgggctg cgccgcgaga
     5101 tcgacgagca cgttgtgcag cgtgacggtg tcgacatgga ccgtcttgcg cagcagcggc
     5161 ctcagctgga cgccgagctc ggcggattcg atcgtgatga acggcttgcc gtctttgcct
     5221 gcgatcgcga tgcccgtggc ggtcagcgag ccgaacgggc tgccgtcgac gcggccgacg
     5281 gtcgagccgg gaaagtcgtc ttgcaacgcg gactcgagct ctttgcgaat gcgctcgcgg
     5341 ccgaattttg tgtggacgat gccgatcgcg atcgccgcga gcaagatgac gagcgcgatc
     5401 gagccgagga cgatgcgacg cgtccagcgg aagatcgagc gcagcgtgat cttgcgagcg
     5461 ggcatgcgtt agaacgcctc gccgacgctg aggatgaagt tgaagagctg gccgcgcgat
     5521 ggctcgttgc cgccggtgcg gttgatacga cgtgcgacct cgaggcggat tggaccgatg
     5581 ggcaggtaga agaagcgcag acccgcgccg accgcccaat gcaggtgcgt gagatcgagg
     5641 tcggcgggct tggaggtcac gtcgccgccg tcgaggaaca cgacccagcc cgctttgatg
     5701 cccagcggct cccagtggcg gcgaacttcg atcccggtct cgaagaggcc cgcgccgccg
     5761 acgacgacgg agctcgtaat gttcgtgccc actacgcccg tcgctgggtc gcgctcgact
     5821 gcgatcactt cgggcgagag gcggcgatcg gcgaagccgc gctggctcgc tgcaccgccg
     5881 gcgtagaagc gttcggtcgg cgggacatcg ccgaggatcg cgccgaaccg cgcatgcgcc
     5941 gcgaacactg cgccaccgag cgagtagtaa ccgcgaagct cggggatcag ttgcagatag
     6001 ttgaaggcgc cgcccgcagc gactgtgcct tcgctcacct tgatctgggc gtagccaccg
     6061 acggtgggtt cgacgggatt atcgcgaagg tcgagctcga gcgcttgtgt gaatgcgccg
     6121 aggcgttcgt cttccaggag gccgagcgat tcggcgcgcg ttggatcgta agcgacctcg
     6181 gtcgcgccag cgtcgggcgt cggatcgagc ggcggcacga ggttgccgaa cgtgtagttc
     6241 gcgaactgcc agccgaggtg accctgcagg cgcggcgtcc cgagcggcga gctaatgccg
     6301 aggcgaacga gcggtccgta ttcgacgaag ccttcgagag tcacgtagtc gtagccacct
     6361 tgcagctcac ctttgacgcc ggggcggagg aaatcttgct gcacggccga gccgagaaca
     6421 cgaatgcggg gttcgagcgg aaagctgtcg tcggagtcgc tcatcttgca ttcatcgaag
     6481 cccaccagac ccagcaccgg gcaatggtcg cgcagaacga ccagcgcggg gcgagcttcg
     6541 agcgacagcg tcgacaaagg agtgggccac ccggtgtggc tcagagagcc tcgcaggcgg
     6601 acgttgtagc taaggatgtc gattccggca cctatgccgc cgcgcgcctc ccagtggagg
     6661 gcttcggtca gctggattcg aacggggacc gacgcatcga gcgtcgtgcg atcggcatcg
     6721 actcgcaccg ccgagaacat gccgatgcca tagattgcct cctgtgattg cgccagcgct
     6781 ttcgtcgaat agccgtcgcc cggcttgaag gtcaggcgag cgcgcgcagc gtcggcgagc
     6841 acgccatcga cgcccacgat ctcgatcgcg ccgaacgtcg tgcgcgggcc cggatcgaat
     6901 gcgtactgca gcgtcgcggt gttcgcggct ttgtcggcga gcacggcggc ttgcatgacc
     6961 gcgtgcgcgt agcccgcatt ctcgacgagc gcgagcatcg gcgtcttcgc gccgtcgaat
     7021 gcgtcgtacg agaaggcggc gccgtctttc accggcacga gcgcgcgcgc ttctgcgagc
     7081 ggaacctcgg gcggtaggcc gatgatgtcg accttcgctt ttgcctgcgg cccttcatcg
     7141 acgtgaaaga tcgcgatctg cttgtcgccc ttgcgctcga cgcgcggagt cacttttgcg
     7201 gcgaagtagc cgtgcttttg aaacgtgccc tcgatgcgcg tgacgtcgag gttgagctgg
     7261 tactcgtcga gcgcgcgctt gcctttggct cggttcagcg cgaggccggg gatgatgttc
     7321 ttggggttga agtcgagcgc tttcttgttt gcctcgatgc ggatctcggc caggtactcc
     7381 tcgcccggac ggcgcaccgt cttctggccg ccaccgcagg ccgcgacaat cgcgaccacg
     7441 gcggtcagag cgacgctgcg cgacccggac atcccgtgat agtacgcggc catgctcgga
     7501 atgaagacgc tcttgccgct cgtcctgttc actgccaccg caaccgcgaa gggcttgacg
     7561 atcgaggaca tgctcgcgat gcagcgagtg agcgacccgc aggtatcgcc cgacggcaag
     7621 tgggtcgcat tcagcgtgcg cgataccgac tacgacgcga ataaaggccg cttcgatgtc
     7681 tacgtcgcag cagtcgatgg cagctcggtg acccggctca cgaccgacga ggcgaacgat
     7741 accgacgcca agtggtcgac cgatggcaag tggctctact tcatgtcgac gcgctctggg
     7801 agcggccagg tgtggcgcat ccggccaacg ggcggcgaag ccgagcaggt aacgaagcta
     7861 gacaccgaca tcaacgcgtt tcgcgtcatg cccgatggca agcgcctcgt cctcgcgatc
     7921 gatgtctggc ccgacgcgaa aacgctcgcc gattcgatca agcgcgacga agcgaaggac
     7981 aagtcgaagg tcaaggcgcg cgtctatgac cagctgctgt ttcgtcactg ggacacgtgg
     8041 gaggacggca agttctcgca cctcttcatg tggtcgagcg acagcgacgt gaaggacctc
     8101 accgcgggac tttcgaccga tacgccgatt catccgttcg gcggcagcga gcagttcacg
     8161 gtttcgcccg acggcaagtt gctcgcgtac gtcgcgcgcg tcggcggcaa agagatcgcg
     8221 tacacgacga acacggacat cttcctgacg tcgacggacg gcaaaggcaa ggccgcgaac
     8281 atcaccggcg acaacaaggc gtacgacttc gacccggtgt ggtcgcgcga cggcaagtcg
     8341 atcgcattca ccgcgatgaa gaagcccggc ttcgaagccg atcgtacgcg catcacgatc
     8401 tacgacgtcg cgagcaagaa gcagcgcgtc gtcacggaag gctgggaccg ctccgcagga
     8461 acgctcacgt ggagcgccga cggcaagacg atctacacga acgccgagaa cgtcggcaac
     8521 gaggcgatct ttgcaatcga cgtgacgaac ggaaccgcga agccgctcgt cgagaaaggc
     8581 tcgaataact ccgcgcagct cgccggcgat cgcctggtct tctccaagga cacgctcaag
     8641 atgccggc
//
LOCUS       DATLBZ010000340        15416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4099643, whole genome shotgun sequence.
ACCESSION   DATLBZ010000340 DATLBZ010000000
VERSION     DATLBZ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4099643"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VMZ53_17530"
     CDS             <1..476
                     /locus_tag="VMZ53_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30316.1"
                     /translation="AGIYVDVETDGRTLMIQRSAVQAEDDSLPAEARPSETQSTVELK
                     RLASKAPEVFERLARDGIPFETFRALYHGEAEPPDPARGPVAWRLSLAPYRQWLSEDD
                     RFEPTPEEAATMRRFEVCYEARRDRATWKEAARVALEEVPMVLATVLVPVQSVSS"
     gene            complement(458..1552)
                     /locus_tag="VMZ53_17535"
     CDS             complement(458..1552)
                     /locus_tag="VMZ53_17535"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS30317.1"
                     /translation="MPKVLVSNNSELLRHFTAPPFGRLGLQLLVAGNATEAMSLFKTE
                     EPPLVVLDAEADGYSVAKQCKAHNPGTRVILVAGKRLSGDQMREVSASGCDELLIAPM
                     TADELHDVIAIQLGEPRPGTEAFGVAIALAGKKVDATFSNLSIDGVRVVVAEPVSEGQ
                     TVDVTITPEGEVPVLIKGSAVWAQPRDGKTVVGIAFGALDDKARIQLAKLTQWQVVKD
                     GERTRVVLRGDFTEATRFDELMPAMVGRVVFDTAQVTYMNSLGVRAWCEFLRQARIQG
                     YEFHACSVPFVLQASMVRDVIGRGTVTSFFAPFHCISCDHQEERLLQSAAMLASNLEP
                     PTFKCPNCGGALEFDDLPERYFAFLQDDTD"
     gene            complement(1587..3332)
                     /locus_tag="VMZ53_17540"
     CDS             complement(1587..3332)
                     /locus_tag="VMZ53_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30318.1"
                     /translation="MRFCPFCSAENADEQASCVACGRRLPPLPPRRAGSKGQPTGITL
                     SRPPKTPTVPPPIPVAAMPVAAMPVAAMPAPAMPAPAMPPSKDSAPALPPPPVVPVPS
                     SQGLLDAITPGPAAERRPPSPPESRIIADRRGANPKPLTTPPPMTPPSVFDRPKTSSV
                     PPPIPGSGPNRAPSSGPFAVPSTSAFAADTAPSAAPLDIAPKSALEISSSRTTPAGLQ
                     PPPTRIHRAENLVDRPFAPPKVAPVPAIPEPNLINAAKYAYKFGIARWQRRGAIKQLG
                     TEIKQDTEALDQVLGLLGGAARNARVEGRVFSAENTAITAAEERVASLNRESNEVDGR
                     KVEENSKFVDVERERNEKLVEAERMVEEAQKELQHLEAQRRGLRDKRKELDRRQKAFL
                     KNADDHDRQAGAAPLGDQRQELRRAAEGHRKEAAALEPEKQDIDRRLASLERPIGEAT
                     ARLDAAKAELDAAKRSLNDAREGHTHRLAELDAEQKRKSREVALAEGEIQRRLVTLGT
                     LVNLNRIEDPQFAELYQRIDRLRGAITARTTEIEKLTAEREAYDRGTLVRGVATIGGA
                     FIVLIALIVILRAIL"
     gene            complement(3332..4858)
                     /locus_tag="VMZ53_17545"
     CDS             complement(3332..4858)
                     /locus_tag="VMZ53_17545"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HUS30319.1"
                     /translation="MNGDIFTAHGHQPALAIKNGTIVGFGDVSLYRSPRTVDLHGASV
                     IVGLTDAHCHLYGLGMDIENVSVRQLDSEAAVVKVVADAAKQRPASEWLVGRGWDQNR
                     WQGAQFPTKASLDAAVSDRPVLLRRVDGHAIWVNTKALEAAKITKATPDPAGGKIVRD
                     AKGEPTGVFIDNATDLVEVAMPPASPEARERRILAAAKIAVENGFTSVHEMGIEQATA
                     DVYRKLAEQHRLPLRVHAYLAGDPSHSDALRANGPQPGLDRFEMKGVKFFIDGALGSR
                     GARLYADYDDDKGNRGLWVTEPSALTKAVDDAVTGGWQTAIHAIGDAGIGAVLDAYEA
                     ANAKHPGEHRLRIEHLQVFAARDLPRIIATHSIASMQPTHATSDMPWAEKRVGAERIK
                     GAYAWRTMLDNKVPLAFGSDFPVEEVSPLLGLYAAVSRQDAKGQPPGGWYPAQRMTLD
                     EAVAGFTSGAAYAAFAETGYDTRADLTIFDGQLTPQTLLDRKVAVTIVGGEVVYGELH
                     "
     gene            complement(4920..6431)
                     /locus_tag="VMZ53_17550"
     CDS             complement(4920..6431)
                     /locus_tag="VMZ53_17550"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HUS30320.1"
                     /translation="MRHVVMGLLATCWMAGLAPAQTPVPVPADDLPAQQSDPQPTPVP
                     VPVETHTDPPPPEAAKPEPAKPEPPKPIQSAEIPADGAEIKQIIVEDNKKTTSDTVEL
                     IARLEVGDHWTPDMVDVVKSRLVSSGLFKDVEVYWTKADGGVAVHILAKDKHSWVIAP
                     AAYNQPTNKGVGVGFGENNLFGQNQKLLLYGQIATGDSFFIGAWLIPSIGGTRLYGQL
                     DTYLKSARNYEYAPPDSYLIDRDAGDLAPVRESRMHYFNLGGKLGIELWRGVKIDARF
                     RGAKVSYPSSTTPDEDMPMPLAKPGAEGYDVSNEWNLTIDRRANWYGIQDGHKYNFSF
                     EHSLPSLGSDFVYWMAGASAFKAVRVLERHNLILKGAVNYGSNLPFQQEYVTGGTSMR
                     GWLNNEFRGNLRALANLEYSVPVFTISGFSVRALAFYDSAYTTFTKTDEVAAQRNYWP
                     GSQVRGLDGFKNSVGVGTRFYLRQIVLPLLGLDFGYGLEARDYQIYLAIGLTD"
     gene            6471..6956
                     /locus_tag="VMZ53_17555"
     CDS             6471..6956
                     /locus_tag="VMZ53_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HUS30321.1"
                     /translation="MNPAEVATSLLIERTLQDSPAYRKIDDSLYVIKQGSAYVMINVV
                     PWGENKAMVRCTSQLVKGVDVDGPLAKQLLELNAHLRFGAFAWEPSEQVVLFTHTILG
                     GTTLDPEELMATLRDVALIADEYDDKLMKKYGGQRMIDLLEEAAMERIMEKDPKGFQF
                     E"
     gene            7428..8924
                     /locus_tag="VMZ53_17560"
     CDS             7428..8924
                     /locus_tag="VMZ53_17560"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HUS30322.1"
                     /translation="MRRLFVRSMVIAFVSFALLFGIELFVELWRAGFPSWGAPEWAES
                     NPTKLVDILSPIARAYNNVLAMVLATIGLAIPLTANMHTPKLIEMFLKDRINRAVLSL
                     MAAGAAHVLWVEYIIGPNFAPTWAFTIAIIFAIFGWAILLPYFFYVVRFVDPSRLLVR
                     LKDSTMFTIAKVCERETDAADAQTDASRAVNQIGTIIIKSLDRNDRDVAAEGTWAIKQ
                     MLDDYDKYKSRMPKDWFKVDRADFVGLSDEALEMMTEDRTWFEMKCLQQIEHGFLRAL
                     HGANDTVSTFSDATRVIACRADLHHDEHAIRLCIRFFNNYLREAIKARNLRAVYDVFH
                     QYRRLGRDLVDRPELLRAIGNHFAYYAVMARTYGMLFAPQLALFDLGYVTRRAYERAS
                     PVSGDLLKIVLALPHRTGNDLHSMAVKAKLILGGFFVENKLEAEADVVRKNLNDVDAT
                     HIERAESELLAAERSFFEVTDRQLNLEYVPPERREPLKKFCDSLSATG"
     gene            complement(8927..9358)
                     /locus_tag="VMZ53_17565"
     CDS             complement(8927..9358)
                     /locus_tag="VMZ53_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30323.1"
                     /translation="MSEAADGLDDVDDEVIIKVTDYLDGSLPAAEREDVGKKIASDAA
                     WKRTHDEMVEARKMISGLRKAKPPETFVEDVTGTINKRSGGAFFGRRTLGDRVPFGVL
                     LILAIVALAVIFFLMRSSSTGSLKVDKKQQTQEPTKPVPTP"
     gene            complement(9351..9956)
                     /locus_tag="VMZ53_17570"
     CDS             complement(9351..9956)
                     /locus_tag="VMZ53_17570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS30324.1"
                     /translation="MSELERSLLKRLRDRDERAFRELISNHRDRVYNITFRMLGNRAE
                     AEDVAQEVFITVFKTIDTFREESKFSTWLYRVAVNHCKNRIKYLARRYDRSREELDET
                     THGGESSSSGNGVISSPPPSAPDRALEGAQMEKVLQEAIATLDEDQRTVVILRDVEDL
                     SIEEICEITGLADGTVKSRLHRARLVLRKKLQRQGIGENHE"
     gene            10221..10670
                     /locus_tag="VMZ53_17575"
     CDS             10221..10670
                     /locus_tag="VMZ53_17575"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUS30325.1"
                     /translation="MNTRLYVGNLSFNTTADSVRAAFQPFGNVSDVHLVTDRETGRSR
                     GFAFVTMGTPEEAAKAIESMDGKQLDGRPLRVNEAEQRQARGGGGGGGGYRGGGGGGY
                     GGGGGGGGYGGGGGGGGDWGGGGGGGDDRGGGRGGRGRGRGGGGDRW"
     gene            complement(10726..11625)
                     /locus_tag="VMZ53_17580"
     CDS             complement(10726..11625)
                     /locus_tag="VMZ53_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30326.1"
                     /translation="MRLVRRSLVVGLSLIGSLIGTAHADGDALVDALGPREVAMGEAL
                     RASATGASAIGMNPAGLPLNRELVFEGGYGYRISDEASILGVSACDSTNAAPGCFYYS
                     YAANDMHRTHVAGMSLAYPITPRVALGSSLKYFNFKSDIMTEPKASGFTNDLGMTLRL
                     TDLVNVGVAGYNLLGKESTEFNRAVGTGVMARPIPTLSVSFDARWKLEDNDHTMRYGG
                     GAELFMRTGNGQTGFPLRAGALHDNGLDATFVSAGGGIATMKFAVDVAARFAVSGPEA
                     TTIIASLRFFGPRLAAPSTDGTQ"
     gene            11682..12191
                     /locus_tag="VMZ53_17585"
     CDS             11682..12191
                     /locus_tag="VMZ53_17585"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUS30327.1"
                     /translation="MRNTIDALELLTSQHDEVEMLFSEIEDSDDADEKAELFRELADK
                     LAAHSTMEEKLFYPSVMAENTADALLEATEEHLAAKRILADMLEMDPDDDRFDAKLSV
                     LKENVRHHAREEEEGKLFPKVRRMFSADELAGLGNDCLAFFEQLLERNPRMNVPAETA
                     EAAPLDLNF"
     gene            12299..13246
                     /gene="egtD"
                     /locus_tag="VMZ53_17590"
     CDS             12299..13246
                     /gene="egtD"
                     /locus_tag="VMZ53_17590"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HUS30328.1"
                     /translation="MSVVARAFERSDEVVTGLQSHPKKLPSRLLYDAHGADLFEQITQ
                     LDEYYPTRCELRLLDAHLPAIAADVGVAARVIEPGSVVSIKTKRLLRALDRPAMYIGV
                     DVARDALEYGTKVLRAEHRDLDVHALVADFTRPFALPSVRRPIGKTLVFFTGSTIGNF
                     EPHEAVAFLSSLQRVAGTNARLLLGADGTRDPETLVPAYDDGDGVTARFAMNALAHLN
                     RTRGASFDPGKFRYRAIWNEMYSRVEMQLVSQCDQLVRVGGDDVRFRTGEPLVTEYSY
                     KHTPHAMRGILLAAGWHVRQVFTATEQPMRLWLCEPRTV"
     gene            13258..13815
                     /locus_tag="VMZ53_17595"
     CDS             13258..13815
                     /locus_tag="VMZ53_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30329.1"
                     /translation="MAKSESPLIQAATEFDEALAVYSRLGDLFLKTPLSSLKHLERAN
                     QTLGELADCEQRLQDTGKKLIEALTAARQRQEQLSQDVIAQAPMLKERNAQLRELMTQ
                     MGALAADVQSVNALVSAKNGDQAQPPDADAGEVSAKVLELSKRAEGLAASARDVDFAE
                     LADQAHSLHQRLKAIGMKLGKATGN"
     gene            13887..>15416
                     /locus_tag="VMZ53_17600"
     CDS             13887..>15416
                     /locus_tag="VMZ53_17600"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HUS30330.1"
                     /translation="MRLRSVLASLLFSACTIQTTPAPRYGYQQQPPPTQQPPPTQVQT
                     QPPPIQPPPPQPQPVYYDEPGYYDVNVNFQGDVVWNVDVFYDQLDPYGTWYNDQTYGW
                     VFVPQQQSYVPYSNGYWKYTDYGMLWVSADPFGWATDHYGRWVYINRWVWAPDLTWGP
                     AWVNWRAGDGWIGWAPAGYTQDAYVPEDHWHFCRAPDLFAVDLRTRFITGSTKTYLTT
                     SNPIVRYNKYKNYQWVAGPSENWFRRYNVYFKKDRVDPNEGGWFEKARRQEADRRAKE
                     RKKEWDERRKRDAEIRKMQEEQLREKREEQKRLAEEQRKLAEQAKRQEEDRQRLEAQR
                     KKFEEEQARARNDAERKRLEEERRRLEEQQRKQDEERRKLAEQQARQQAEEAKRRDEE
                     QRKQREEQERQKAEQQKRLEEQQRKEKEDEQKRQAEIEKRRQEDQKRLEEQQRKAAEE
                     QRKRDEEAKRQAEQDAKRAEQEKRKQEEEQRRQDEQKRKQAEQDAKRAEQEKRKQEEE
                     NR"
BASE COUNT         2753 a         4765 c         5217 g         2681 t
ORIGIN      
        1 gggccggtat ttatgtggat gtcgagaccg atggtcgaac gctcatgatt cagcggtcgg
       61 ccgttcaagc agaggatgac agcctgcccg ccgaggcgcg tccatcggag acgcagagca
      121 ctgtcgagct caagcggttg gcgagcaagg ctcccgaggt cttcgagcga ctggcccgcg
      181 acggcatacc cttcgagacg tttcgcgccc tctatcacgg cgaagcggaa ccgcccgatc
      241 cggcccgtgg acccgtagca tggcgattgt cactggcgcc ttatcgacag tggctgtcgg
      301 aggatgatcg attcgagccg acccccgagg aggctgccac gatgcggcga ttcgaggttt
      361 gctacgaggc gcggcgtgac cgcgcgacgt ggaaggaggc tgcgcgcgtt gcgctcgaag
      421 aggtccccat ggtcctcgcc acggtcctcg ttcccgttca gtcggtgtcg tcttgaagga
      481 atgcgaagta gcgctcgggg aggtcgtcga attcgagggc gccgccgcag ttggggcact
      541 tgaacgtcgg cggctcgagg ttcgacgcga gcatcgcggc ggattggagg aggcgttcct
      601 cttggtggtc gcagctgatg cagtggaacg gcgcgaagaa cgatgtgacc gtgccgcgcc
      661 cgatcacgtc gcgcaccatc gaggcttgca gcacgaacgg gacggagcag gcgtggaact
      721 cgtagccttg gatgcgggcc tggcgcagga actcgcacca cgcgcgcacg ccgagcgagt
      781 tcatgtacgt cacttgggcg gtatcgaaga cgacgcggcc gaccatcgcg ggcatcagct
      841 cgtcgaagcg cgtggcttcg gtgaagtcgc cgcggaggac gacgcgtgtg cgctcgccgt
      901 ctttgacgac ctgccactgc gtgagcttgg cgagctggat gcgagctttg tcgtcgaggg
      961 cgccgaatgc gatgccgacg accgtcttgc cgtcgcgagg ttgggcccag accgcgctgc
     1021 ctttgatgag gaccgggact tcgccttcgg gcgtgatggt cacgtcgacg gtctggcctt
     1081 cggagaccgg ctcggccacg acgacgcgga cgccatcgat cgagagattc gagaacgtcg
     1141 cgtcgacctt cttgccggcg agcgcgatcg cgacgccgaa cgcttcggtg ccggggcgag
     1201 gctcgccgag ctggatcgcg atgacgtcgt gcagctcgtc ggctgtcatc ggcgcgatca
     1261 ggagctcgtc gcagccgctg gcgctgactt cgcgcatttg gtcgccggac aggcgcttgc
     1321 ctgcgacgag gatgacgcgc gtgcccgggt tgtgcgcctt gcattgcttg gcgacggagt
     1381 agccgtcggc ttcggcgtcg aggacgacca gcggcggctc ttcagtcttg aacagcgaca
     1441 tcgcctcggt ggcgttgcca gccacgagaa gctgcagccc gagcctgccg aatggcggcg
     1501 cggtgaagtg ccgcaacagc tcgctgttgt tcgagaccag taccttaggc acctgtacct
     1561 gtgtccaacg ttactacagt tgggcgttac aggatggcgc gcaagatgac gatcaacgcg
     1621 atgagcacga tgaaggcacc accgatagtt gcgacgccac ggacgagagt tccacgatcg
     1681 taggcctcgc gctcggccgt cagcttctcg atttcggtgg tgcgggctgt gatggcgccg
     1741 cgcaggcgat cgatgcgctg gtacagctcc gcaaactgcg ggtcctcgat gcgattgagg
     1801 ttgacgaggg tgcccagcgt gacgagccgg cgctggattt cgccctcggc cagcgcgacc
     1861 tcgcgagatt tgcgcttctg ctcggcgtcg agctcggcga ggcggtgggt gtgcccttcg
     1921 cgggcgtcgt tgagactgcg cttggcagcg tcgagctcgg ccttcgcggc gtcgaggcgc
     1981 gcggtggctt cgccgatcgg acgctcgagc gacgcgagcc ggcgatcgat gtcctgcttc
     2041 tcgggctcga gcgcggcagc ctctttgcgg tgaccttccg ctgcgcgacg cagctcttgg
     2101 cgttggtcgc cgaggggagc tgcgcccgct tgccgatcgt gatcgtcggc gttcttgagg
     2161 aaggccttct ggcgacggtc gagctctttg cgcttatcgc gaagaccgcg gcgctgcgcc
     2221 tcgagatgct gcagctcctt ctgcgcctcc tccaccatgc gctccgcctc gacgagcttc
     2281 tcgttgcgtt cgcgctcgac gtcgacgaac ttggagttct cctcgacttt gcggccgtcg
     2341 acttcgttcg actcgcgatt gagactcgcg acgcgctctt cggcggccgt gatggcggtg
     2401 ttctcggcgg agaacacgcg accttcgacg cgtgcattgc gtgccgcgcc gccgaggagg
     2461 cccagtacct gatcgagagc ttcggtgtcc tgcttgatct cggtgccgag ctgcttgatc
     2521 gcgccgcgcc gctgccaccg cgcgatgccg aacttgtacg cgtacttcgc ggcgttgatg
     2581 agattgggct ccgggatcgc ggggaccggc gcgaccttgg gcggggcaaa cggccgatcg
     2641 acgagattct ctgcgcgatg gatgcgcgtc ggcggcggtt ggagtcccgc cggcgtcgtg
     2701 cgcgacgagc tgatctcgag cgcggatttc ggcgcgatgt cgagcggcgc ggcagaaggc
     2761 gcggtgtctg ctgcgaacgc gctcgtgctc ggcaccgcga atggacccga gctcggcgcg
     2821 cggttcgggc cggagcccgg aatcggcggc gggaccgacg aggtcttcgg gcggtcgaac
     2881 acggatggcg gcgtcatcgg cggcggcgtc gtgagcggct tgggattggc tcctcgacgg
     2941 tccgcgatga tgcgggattc tggcggcgac ggcggacgac gctcggcggc ggggccgggc
     3001 gtgatcgcgt cgagaaggcc ctggctcgac ggcaccggca ccacgggcgg cggcggcaac
     3061 gcaggcgcgc tgtccttgct cgggggcatc gcaggcgcgg gcatcgcagg cgcgggcatc
     3121 gcggctaccg gcatcgcggc taccggcatc gcggcaaccg ggatgggcgg tggaaccgtc
     3181 ggcgttttcg gtgggcgcga gagcgtgatg cccgttggct ggcctttgct gcccgcacgg
     3241 cgcggcggaa gaggcggcag acgacgcccg catgcgacgc aggacgcctg ttcgtcggcg
     3301 ttctcggcgc tgcagaacgg gcagaagcgc attagtgaag ctctccgtac acaacttcac
     3361 cgccgacgat cgtaacggca actttgcggt cgagaagagt ttgcggcgtg agctggccgt
     3421 cgaagatcgt gaggtccgca cgggtgtcgt agccggtttc agcgaacgcg gcatacgccg
     3481 cacccgacgt gaagccagcc actgcctcgt ccagcgtcat tcgctgcgca ggataccaac
     3541 ctccaggcgg ttggcccttc gcatcttggc gcgagacggc ggcgtatagg ccgagcagcg
     3601 gcgagacctc ctcgacggga aagtccgagc caaatgcgag tggcaccttg ttgtcgagca
     3661 tcgtgcgcca cgcgtacgcg cctttgatgc gctcggcgcc gacgcgtttc tccgcccacg
     3721 gcatgtcgct cgtcgcgtgc gtcggctgca tcgatgcgat cgagtgcgtc gcgatgatgc
     3781 gcggcagatc tcttgccgcg aacacctgga ggtgctcgat gcgaaggcga tgctcaccgg
     3841 gatgcttcgc gttcgcggct tcgtacgcgt cgagcactgc gccgatgcca gcgtcgccga
     3901 tcgcgtggat cgcggtctgc cacccgccgg tgacggcgtc gtcgacagct ttcgtcagcg
     3961 cggacggctc ggtgacccac agaccgcggt tgcctttgtc gtcgtcgtaa tcggcgtaga
     4021 ggcgcgcgcc gcgcgagccg agcgcaccat cgatgaagaa cttcacgccc ttcatctcga
     4081 agcggtcgag acctggctgc ggcccgttcg cgcgcagcgc atcactgtgc gacggatcgc
     4141 cggcgagata ggcatgcacg cgaagcggca gacgatgctg ctcggcgagc ttgcgataga
     4201 catcggccgt cgcttgctcg atccccatct cgtgcaccga cgtaaaacca ttctcgacgg
     4261 cgatcttcgc cgcggcgagg atccggcgct cgcgcgcctc tggagaggcc ggcggcatcg
     4321 ctacttcgac gaggtccgtg gcgttgtcga tgaagacacc ggtgggctcg cccttcgcat
     4381 cgcggacgat cttgccgccc gcgggatccg gcgtcgcctt cgtgatcttc gccgcttcga
     4441 gcgccttcgt gttgacccag atcgcgtgac cgtcgacgcg acgaagcagc acgggcctgt
     4501 cgctcaccgc cgcgtcgagt gacgccttcg tcggaaattg tgcaccttgc cagcggttct
     4561 gatcccagcc gcgaccgacg agccactcgc tagccggccg ctgcttcgct gcatccgcga
     4621 cgactttgac gaccgctgct tcggaatcga gctggcgcac cgagacgttc tcgatgtcca
     4681 tgccgagacc gtagagatgg cagtgcgcgt cggtgaggcc gacgatgacg ctcgcgccgt
     4741 gcagatcgac ggtgcgcggc gatcgataca gcgacacatc gccgaatccg acgattgttc
     4801 cgttcttgat ggcgagcgcc ggttgatggc catgcgcggt gaagatgtcg ccgttcacga
     4861 gaacgagatc gacatctcgt gcacctggac gcttcggttc aggtgcgcag gctgcgaggc
     4921 taatcagtaa ggccgatcgc cagatagatc tggtagtcac gagcttcgag accataccca
     4981 aaatcgaggc ccagaagggg cagcacgatc tgccgcagat agaagcgcgt gcctacgccc
     5041 accgaattct tgaatccgtc gaggccacgc acctgggatc cgggccagta gttgcgctgc
     5101 gcggcaacct cgtccgtctt cgtgaacgtc gtgtacgcgg agtcgtagaa cgcgagtgcg
     5161 cgaaccgaga agccgctgat cgtgaatacg ggcaccgagt actcgaggtt ggcgagcgcg
     5221 cgcaggttgc cgcgaaactc gttgttgagc cagccgcgca tcgacgtgcc gccggtgacg
     5281 tactcctgct gaaacgggag attgctgccg tagttcacgg cgcccttcag gatcagattg
     5341 tggcgctcga gcacgcggac cgccttgaac gcgcttgcgc cggccatcca gtagacgaag
     5401 tcggagccga gcgacggcag cgaatgctcg aagctgaagt tgtacttgtg gccgtcttga
     5461 atgccgtacc agttcgcgcg gcgatcgatc gtcaggttcc actcgttcga gacgtcgtag
     5521 ccctcggcac ctggcttcgc gagtggcatc ggcatgtctt cgtcgggcgt cgtcgagctg
     5581 ggatagctga cctttgcacc gcgaaaccgc gcatcgatct tcacgccgcg ccacagctcg
     5641 atgccgagct tgccgccgag gttgaagtaa tgcattcgcg attcgcgaac gggcgcgaga
     5701 tcgccggcat ctcgatcgat gaggtagctg tcgggcggcg cgtactcgta gttgcgcgcg
     5761 ctcttgagat acgtgtctag ctggccgtag agccgcgtgc cgccgatcga cggaatgagc
     5821 cacgcgccga tgaagaacga gtcacccgtc gcgatctgcc cgtagaggag aagcttctgg
     5881 ttttggccga agagattgtt ctcgccgaag ccaacaccga cgcccttgtt cgtcggctgg
     5941 ttgtacgcag caggcgcgat cacccagctg tgcttgtcct tcgcgaggat gtgaaccgcc
     6001 acacctccgt cggctttcgt ccagtacacc tcgacgtctt tgaacaggcc gctcgagacg
     6061 agccgcgact tcacgacgtc gaccatgtcg ggcgtccagt gatcgccgac ctcgaggcgc
     6121 gcgatcagct cgaccgtatc cgacgtggtt ttcttgttgt cctcgacgat gatctgcttg
     6181 atctcggcgc catcggccgg aatctccgcg ctctgaatgg gcttgggcgg ctccggcttg
     6241 gcgggctcgg gcttggccgc ttcaggcggc ggcggatcgg tgtgcgtctc gactggcacc
     6301 ggcaccggcg tcggttgcgg atcgctctgc tgcgccggca gatcgtcggc cggcaccggc
     6361 acgggagttt gcgcgggggc gaggccagcc atccagcagg tggcaagcag gcccattacg
     6421 acgtggcgca cgcgacgagt attgcacggc tcccgtgtta acaacggcac atgaaccccg
     6481 ccgaagtcgc gacctcgttg ttgatcgaac gcacactcca ggacagcccg gcgtatcgca
     6541 agatcgacga ctcgctgtac gtcatcaagc agggctccgc gtacgtgatg atcaacgtcg
     6601 ttccgtgggg cgagaacaag gcgatggtgc gctgcacgtc acaactcgtg aagggcgtcg
     6661 acgtcgatgg cccgctcgcg aagcagctcc tcgagctcaa cgcgcatctt cgattcggcg
     6721 ccttcgcatg ggagccgtcc gagcaagtcg tgctgttcac gcacacgatc ctcggaggca
     6781 cgacgctcga tcctgaagag ctcatggcaa cgctgcgcga cgtcgcgttg atcgccgacg
     6841 agtacgacga caagctcatg aagaagtacg gcggtcagcg catgatcgat ctgctcgaag
     6901 aagcagcgat ggagcgcatc atggaaaaag atcccaaggg cttccagttc gagtgagcgc
     6961 catgggcgct aaagataaaa accagaacca aggcttcggg acgcgcgcga tccagtagca
     7021 tcgcccggaa gagatcccgg tgatcatggg gtgtcttttc ggaacccgtc atgacggcgt
     7081 catgacgctc aagtgtgcga acgcgcgtga atggcgatgc gcgaatgttg tgcttcgcag
     7141 atggcgtcgt gcacaacgcg accccgccgt gcacagcgcg accgcgccgt ggacgcggcg
     7201 cgaacgtcgg cttcatcgac ctacgcccgc cgacgacgaa ttgcggagtt agtcgtcagt
     7261 acagcgtcat gacgggttcc gaaaagacac ctcgtatgaa ccgggatctg cttcgggcga
     7321 cgacgcgagt cgcgagaaga acgttagatc gatcgggaat cacttctaga agcgccggcc
     7381 gggggcgaag tcgctgtcgt tcgcgcgtcg tagtacccta gatcgcgatg cgcagactgt
     7441 tcgtgcggtc catggtgatc gcattcgttt cgtttgcgct gctatttgga atcgagctgt
     7501 ttgtcgagct gtggcgcgcg gggtttccgt cgtggggcgc gcccgagtgg gcggagagca
     7561 atccgacgaa gctcgtcgac atcttgagcc ccatcgcgcg tgcgtacaac aacgtgctcg
     7621 cgatggtctt ggcgaccatt ggtctcgcga tcccgctcac ggcgaacatg cacacgccga
     7681 agctgatcga gatgttcttg aaggatcgca tcaaccgcgc ggtcctgtcg ctgatggcgg
     7741 ccggcgcggc gcacgtcttg tgggtcgagt acatcatcgg cccgaacttc gcgccgacgt
     7801 gggcgttcac catcgcgatc atcttcgcga tctttgggtg ggcgatcttg ctgccgtact
     7861 tcttctacgt cgtgcgattc gtcgatccgt cccgcctgct cgtgcggctc aaggacagca
     7921 cgatgttcac gatcgcgaag gtgtgcgagc gcgagaccga tgcggcagac gcgcagaccg
     7981 atgcgtcgcg agccgtgaat cagatcggca cgatcattat aaagagcctg gatcggaatg
     8041 accgcgacgt cgccgccgag ggcacgtggg cgatcaagca gatgctcgac gactacgaca
     8101 agtacaagtc gcgcatgccg aaggactggt tcaaggtcga ccgggcggac ttcgtcgggc
     8161 tctccgatga agcgctcgag atgatgaccg aggacaggac gtggttcgag atgaaatgcc
     8221 tgcagcagat cgagcacggc ttcttgcgtg cgttgcacgg cgcgaacgac acggtgtcga
     8281 cgttctccga tgcgacgcgc gtgattgctt gccgcgcgga tttgcatcac gacgagcatg
     8341 cgatccggct gtgcatccgg ttcttcaaca actatctgcg cgaagcgatc aaggctcgca
     8401 acctgcgcgc ggtgtacgac gtgtttcatc agtaccgccg cttggggcgc gacctcgtcg
     8461 atcggccgga gttgttgcgc gccattggga accacttcgc gtactacgcg gtgatggcgc
     8521 ggacgtacgg catgttgttc gcgccgcagc tcgcactctt tgacttgggc tatgtgacgc
     8581 ggcgtgcgta cgagcgtgcg tcgcccgtgt cgggtgactt gctgaagatc gtcctcgcgc
     8641 tgccgcaccg caccggcaac gatctgcatt cgatggccgt gaaggcgaag ctcatcctcg
     8701 gtgggttctt cgtcgagaac aagctcgagg ccgaggccga tgtcgttcgc aagaacttga
     8761 acgacgtcga tgcgacgcac atcgagcgcg ccgagtcgga gctgctcgcg gcggagcggt
     8821 cgttcttcga agtgacggat cgccagctca acctcgagta cgttccgccc gagcggcgcg
     8881 agccgctcaa gaagttctgc gactcgctgt cggcgacggg atagcgctac ggcgtaggaa
     8941 cgggcttcgt cggttcttgc gtctgctgct tcttgtcgac tttgagcgag ccagtcgacg
     9001 acgagcgcat caagaagaag atcactgcga gcgcgacgat cgcgaggatg aggaggacgc
     9061 cgaacggcac gcgatcgccg agcgtgcggc gcccgaagaa cgcaccgccg gagcgcttgt
     9121 tgatcgtgcc cgtgacgtct tcgacgaacg tctcgggcgg cttggccttg cgcaggcccg
     9181 agatcatctt gcgcgcttcg accatctcgt cgtgcgtgcg cttccatgca gcgtcggacg
     9241 cgatcttctt gccgacgtct tctcgctcgg cggcgggcag cgagccgtcg aggtagtcgg
     9301 tgaccttgat gatcacttcg tcgtcgacat cgtcgagtcc atcggctgct tcactcatga
     9361 ttctctccga taccttggcg ctgcagcttc ttgcgaagca cgagacgcgc gcgatgcaag
     9421 cgtgacttca ccgtgccgtc ggcgagtccg gtgatctcgc agatttcttc gatcgagaga
     9481 tcctcgacgt cgcgcaagat gacgaccgtg cgttggtcct cgtcgagcgt cgcgatcgct
     9541 tcttggagga ccttctccat ctgcgcgcct tcgagtgcgc gatcgggcgc ggacggcggg
     9601 ggcgagctga tgacaccgtt gccgctgctc gaggactcgc caccgtgcgt ggtctcgtcg
     9661 agctcttcgc ggcttcgatc gtagcgacgc gcgaggtact tgatccggtt tttgcagtgg
     9721 ttgacggcga cgcgatagag ccacgtcgag aacttcgatt cttcgcggaa cgtgtcgatc
     9781 gttttgaaga cggtgatgaa cacttcttgg gctacgtcct cggcctcggc gcggttgccg
     9841 agcatgcgga acgtgatgtt gtagacccgg tccctgtggt tcgagatgag ctcgcggaag
     9901 gcccgctcgt cgcggtcccg caggcgcttc agcagcgacc gctcgagctc agacatgggg
     9961 cagcgcgagc ataaccgtgt gaatcgatgg acacctgggg cgcggacccg gtttcgctcc
    10021 cgaaagggtt tgtgttggct tttgcctgca agcccctgta actttgcatg tgccaccgtt
    10081 cacgggtctc taggctgctc gcatgttcgc gtttggctac ttccggtgct cagtgggcaa
    10141 gagtttcgaa acccgctttt gagcacagat ggccccctca ggggcgggta cgtcacacgc
    10201 agggcctttc gagcaagctg atgaacactc gtctgtacgt aggaaatctc tcgttcaaca
    10261 ccaccgccga tagcgtccgc gcggccttcc agccgttcgg taacgtcagt gatgtccacc
    10321 tcgtgaccga tcgtgaaacg ggccgctcgc gcggcttcgc gttcgtcacg atgggcaccc
    10381 cagaagaggc cgcgaaggcg atcgaaagca tggatggcaa gcagctggac ggtcgtccgc
    10441 tgcgcgtcaa cgaagccgag cagcgccagg cccgcggtgg tggtggcggc ggtggcggct
    10501 accgcggcgg tggcggtggc ggctatggcg gtggcggtgg cggtggtggc tatggcggcg
    10561 gtggcggtgg cggcggcgat tggggcggcg gcggcggtgg tggcgacgat cgcggcggtg
    10621 gccgtggtgg tcgtggccgt ggtcgcggcg gcggtggtga ccgctggtag atcagcgctc
    10681 gaacaagcag tgaagtacga aagccggtgg agacgccggc tttttttatt gcgtgccgtc
    10741 ggtcgaaggc gccgcgagtc gcggcccgaa gaagcgcagc gacgcgatga tcgtcgtcgc
    10801 ctcggggccg ctgaccgcga agcgcgccgc gacatcgacc gcgaacttca tcgtcgcgat
    10861 gccgccgccg gcagagacga acgtcgcgtc gaggccgttg tcgtgcagcg cgcctgcgcg
    10921 gagcgggaag ccggtctggc cgttgcccgt gcgcatgaac agctcggcgc cgccgccgta
    10981 gcgcatcgtg tggtcgttgt cctcgagctt ccagcgcgcg tcgaagctga ccgagagcgt
    11041 cgggatcggg cgcgccatga caccggtgcc gacggcgcgg ttgaactccg tcgactcctt
    11101 gccgaggagg ttgtagccag cgacgccgac gttcacgagg tcggtcagac gcagtgtcat
    11161 gccgaggtcg ttcgtgaagc cgctcgcctt gggctccgtc atgatgtcgg acttgaagtt
    11221 gaagtacttc aggctcgacc cgagcgcgac gcgcggcgtg atcggataag cgagcgacat
    11281 acccgcgacg tgcgtgcgat gcatgtcgtt cgcggcgtac gagtaataga agcagccagg
    11341 cgccgcgttc gtcgagtcac acgcggatac gccgaggatc gaagcctcgt cggaaattcg
    11401 gtaaccgtac ccaccttcaa aaacgagctc gcggttcaga ggcaggcctg ccggattcat
    11461 tccgatggcg gaggcgcccg tagcactcgc gcgcagcgcc tcgcccatgg ccacttcacg
    11521 tgggccgagt gcgtcgacga gtgcgtctcc gtcggcgtgg gcggtgccga tgagggaccc
    11581 gatcagggac agaccgacga cgagagaacg cctgacgagc cgcatgacac gagcattgac
    11641 gcggtttggt cggcggcgca agaaatgcag ccctttgggg catgaggaac acaatcgacg
    11701 ccctggaatt gcttacgtca caacacgacg aagtcgaaat gctgttctcg gaaatcgaag
    11761 acagcgacga tgcggacgag aaggccgagc tgttccgcga gctcgccgac aagcttgccg
    11821 cgcactcgac gatggaagag aagctcttct atccgagcgt gatggccgag aacacggcgg
    11881 acgcactgct cgaggcgacc gaagagcacc tcgcggcgaa gcgcattctc gctgacatgc
    11941 tcgagatgga tccggacgat gatcgcttcg atgcgaagct cagcgtgctg aaggaaaacg
    12001 tccgccacca cgcgcgcgaa gaggaagagg gcaagctctt cccgaaggtg cgccgcatgt
    12061 tcagcgccga cgagctcgca ggccttggca acgactgcct cgcgttcttc gagcaactgc
    12121 tcgagcgcaa cccgcgcatg aacgtgccgg cggagaccgc cgaagccgcg cccctcgatc
    12181 tgaacttcta acgatcgtgg tacggccact gcatcggtga cgtcccgatg cagtcagccg
    12241 ccgacgttct cgtgcgattt caatgcgtgc gcgagcgctc agcgagcgga ggtttcgcat
    12301 gagtgtcgtc gcccgcgcgt ttgagcgctc tgatgaagtt gtcaccggtc tgcagtcaca
    12361 tccgaagaag ctgccgagcc ggctgttgta cgacgctcac ggcgctgacc tcttcgaaca
    12421 gatcacgcag ctcgacgagt actacccgac gcgctgcgag ctgaggctgc tcgacgcgca
    12481 tctgccggcg atcgccgctg acgtcggcgt agcggcacgc gtgatcgagc cgggcagcgt
    12541 cgtgagcatc aagacaaagc gcctgcttcg tgcgctcgat cggccggcga tgtacatcgg
    12601 tgtcgacgtc gcgcgcgacg cgctcgagta cggcacaaaa gtcttgcgcg cggagcatcg
    12661 cgacctcgac gtgcacgcgc tcgttgcaga cttcacgcgg ccgttcgcgc ttccgtcggt
    12721 gcggcgtccg atcggcaaga cgctcgtgtt cttcacgggg tcgacgatcg gaaacttcga
    12781 gccgcacgag gccgtcgcgt tcttgagcag cctgcagcgc gtcgccggca ccaatgcccg
    12841 gcttctactc ggagccgatg gcacgcgcga tccggagacg ctcgtcccgg cctacgacga
    12901 cggcgatgga gtgactgcgc gattcgcgat gaacgcgctc gcgcatctca accgcacgcg
    12961 cggtgcgtcg ttcgatccgg ggaagttccg ctaccgcgcg atctggaacg agatgtactc
    13021 gcgcgtcgaa atgcagctcg tcagccagtg cgaccagctc gtgcgcgtcg gcggcgacga
    13081 cgtccggttc cgcaccggcg agccgctcgt gacggagtac tcgtacaagc acacgcccca
    13141 cgcgatgcgc ggcatcctgc tggcggccgg ctggcacgtg aggcaggtct ttaccgcgac
    13201 cgagcagccg atgcgcctct ggctgtgcga gccgaggacc gtgtagattc gcctcccatg
    13261 gcgaagtccg aatcaccgct catccaggcc gctaccgagt tcgacgaggc gctcgcggtg
    13321 tactcgcgac ttggcgactt gtttctgaag acgccgctgt cgagcctcaa gcacctcgag
    13381 cgcgcgaacc agacgctcgg cgagctggcc gactgcgagc agcgcctgca ggacaccggc
    13441 aagaagctca tcgaagcgct gaccgccgct cgccagcgcc aagagcagct gtcgcaagac
    13501 gtgatcgcgc aggcgccgat gctgaaggag cgcaatgcgc agctgcgcga gctcatgacg
    13561 cagatggggg cactcgccgc cgacgtccag agcgtgaacg cgctcgtcag cgcgaagaac
    13621 ggcgatcaag cgcagccgcc cgatgccgat gccggcgaag tctcggccaa ggtgctcgag
    13681 ctgtcgaagc gggctgaggg tctggctgcg agcgcacgcg atgtggactt cgccgagctg
    13741 gccgaccagg cccactcgct gcaccagcgc ctgaaggcga tcgggatgaa gctgggcaaa
    13801 gcgaccggca actagctgaa acaaggacgg gaatcgagtt accttcgccg tgaattccgg
    13861 catatgccgg gccgtcgaaa ggttctatgc gcctacgcag cgtccttgcg tcccttctct
    13921 tttccgcctg cacgattcag acaactccag cgccgcggta cggctaccag caacagccac
    13981 cgccgacgca gcagccgccc ccgacgcaag tccagacgca gccgccgccc atccagccgc
    14041 cgccgccgca accccagccc gtctattacg acgagcccgg ctactacgac gtcaacgtca
    14101 acttccaggg cgatgtcgtc tggaacgtcg atgtcttcta cgaccagctc gatccgtacg
    14161 gcacttggta caacgaccaa acctacggct gggtgttcgt cccgcagcag cagagctacg
    14221 tcccgtactc gaacggctac tggaagtaca ccgactacgg aatgctgtgg gtcagcgccg
    14281 atccgttcgg ctgggcgacc gaccactacg gccgctgggt ctacatcaat cgctgggtgt
    14341 gggcgcccga cctgacgtgg ggccctgcat gggtgaactg gcgcgcgggt gatggctgga
    14401 tcggctgggc accggccggc tacacgcaag acgcgtacgt gccggaagac cactggcact
    14461 tctgtcgtgc accggatctg ttcgcggtcg atctcaggac gcgcttcatc acgggcagca
    14521 cgaagacgta cctgacgacg tcgaacccga tcgttcgcta caacaagtac aagaactatc
    14581 agtgggtcgc gggcccgtcg gagaactggt tccgccgcta caacgtctac ttcaagaagg
    14641 accgggtcga cccgaacgag ggcggctggt tcgagaaggc gcgccgccaa gaagcggatc
    14701 gccgcgcgaa agagcgcaag aaggagtggg acgagcgccg caagcgcgac gccgaaatcc
    14761 gcaagatgca agaggagcag ctccgcgaga agcgcgaaga gcagaagcgc ctcgccgaag
    14821 agcagcgcaa gctcgccgag caggcgaagc gccaagaaga agatcgccag cgactcgaag
    14881 cgcagcgcaa gaagttcgag gaagagcaag cgcgcgcgcg taacgacgcc gagcgcaagc
    14941 gcctcgaaga agagcgccgc cgcctcgaag agcagcagcg caagcaagac gaagagcgcc
    15001 gcaagctagc cgagcaacaa gctcgccagc aggcggaaga agcgaagcgc cgcgacgaag
    15061 agcagcgcaa gcagcgcgaa gagcaagagc gccaaaaggc cgagcagcag aagcgcctcg
    15121 aagagcagca gcgcaaagag aaggaagacg agcagaagcg ccaagcggaa atcgagaagc
    15181 gccgccaaga agatcagaag cgcctcgaag agcaacagcg caaggcggcc gaagagcagc
    15241 gcaagcgcga cgaagaagcg aagcgccagg ccgagcaaga cgccaagcgc gccgagcaag
    15301 agaagcgcaa gcaagaagaa gagcagcgcc gccaagacga gcaaaagcgc aagcaggccg
    15361 agcaagacgc caagcgcgcc gagcaagaga agcgcaagca agaagaagag aaccgc
//
LOCUS       DATLBZ010000341         7604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2630157, whole genome shotgun sequence.
ACCESSION   DATLBZ010000341 DATLBZ010000000
VERSION     DATLBZ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630157"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="VMZ53_17605"
     CDS             <1..1733
                     /locus_tag="VMZ53_17605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS30331.1"
                     /translation="GEGGGRGGGEGGGEGGGEGGGEGGGEGGARGEGEGGGRGEGGGR
                     GRGEGGGGAGRGGGGEREGGGRVRKVRRRGLWDHQDFLRLWSAQAISAIGSRITRTAL
                     PIIAISTLHQPETSVALLWSLYIIPGLVLALFAGGFVDRTRKRRILIAADLFRAACVG
                     SLTLSWALDLLTMAQLIVVGMFVGAASALFQIADVAYLPTLVSKKQLPEGNAKLEATE
                     GVAEISGPASAGILIGVLGAPLAVALDALSYVWSAAMLGRIRTKEPAPLASASSALSL
                     SAVSASASASLALSASAASASASGSASASLALSASSAASGSGSAAGSAASGAAASAPA
                     GGGSTLARTTLSRTRRDLSVGMRAIFGHPHVRPIVIALLVWSIVGGFFTSLYALYCLR
                     TLDLSPELFGAIVAIGGAGSLAGAFMSRRLVARIGLGPTMIMSAVISVVSGILIPLAR
                     APDWFALVCLGAHQLINDCFSVVFMINATSLRQTVLPRAVLGRANAAFHICMSGPYPI
                     VIIAAGAIAEATSIRTAVWVGMLIGLVVPLLLLSLRSLRKMPEAAASSTDLPVEAASS
                     ASDMTLPLST"
     gene            1941..2759
                     /locus_tag="VMZ53_17610"
     CDS             1941..2759
                     /locus_tag="VMZ53_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30332.1"
                     /translation="MSRLTTLSCSLLVAVSSLLPAAAVAKRMNPNAYDSVMAPAEQPV
                     APSKPIHGGGAPSNGGFAQPPPPLSAGAAAQADIYEPSVAFAPRRSLDRATVRAALVQ
                     ARSRNLSAFRDYQKKGVFPSNSFKGKKLNVWLDADGHFCAAATIIKTSGQDDLVNKVA
                     EQNNFIRLADVKQGPLMDWILTSGFTQDEIAAIQEPFMPVVDRPALEPAKPILVDAKL
                     RKAEDARLMAKYKQVDKQLVKNASKSLDKATDRLMKNPTLAWQLIASTDVDVLE"
     gene            complement(2886..4277)
                     /locus_tag="VMZ53_17615"
     CDS             complement(2886..4277)
                     /locus_tag="VMZ53_17615"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUS30333.1"
                     /translation="MSSTSRIDTSSGAAAAAAAALPSLGEDARRLCQSLLRIDTTNPP
                     GNERAAAEVIANALREVDLVPELLEAEPHRTNLVVRYKGTGKLPPLLLTAHLDVVEAD
                     PSKWRRGPFSGDEFEGCLWGRGAIDMKNMAAMCTAILRRLAATKTRLDRDIIFAAVAD
                     EEAGCDLGSRFLVERHKSKVEAEYAIGESGGFSLHLGESTFYPVQVAEKGFCWVRARI
                     TGEPGHGSMPRHDSVITKLGEALTRIGETSLPTHPTKYLTDFLDALRARQPAVVQPFM
                     KLVARPQLLGRIARLVPGASISRSFLALLSNTASATVVRAGAKTNVIPGVAEFEIDGR
                     TLPGQTDEDLLRELRAVLGPEVELEVMKSAPPIVTEPMASPLFDIIKRQVESREPDAE
                     VVPYLIPGFTDAKYFTLMGARWYGFSPVKIEKSSGIKFAEMFHGHDERVPVAGLAWGA
                     EVLDAVVREISVA"
     gene            complement(4975..5562)
                     /locus_tag="VMZ53_17620"
     CDS             complement(4975..5562)
                     /locus_tag="VMZ53_17620"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HUS30334.1"
                     /translation="MIATPERNPDHPDVIDLDEHEPDVFDAPLRSYAQHRLRQLARYC
                     VPVLALMLLVVSMDGRWRVASAQPTTAKAGPPTRDAGKTPSAKPRAQASAHATSAEAS
                     AQDESSVDEPPERPIKKPAKDLVGKINLNTANEGELMRLPTVGPAKADRIVTWRKKNG
                     GFRRVADIRRVKGFGYKTFKRLEPFLDVKGETTLH"
     gene            complement(5588..6079)
                     /locus_tag="VMZ53_17625"
     CDS             complement(5588..6079)
                     /locus_tag="VMZ53_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30335.1"
                     /translation="MFQEKKKMFKVISPIDKRDGSKFWMRCGVGYRNKDESINMYIDA
                     LPVNANKDGRPLTLQLREVTEEEMRERSEKRASSGDRGGGGSDRGYSDRGYSDRGYSD
                     RGGASSSSSSSSSYSARGTLGRPSTFDPPDPNHIPMIPGAGGLDHLNALNAQDNNNDQ
                     PPF"
     gene            complement(6289..>7604)
                     /locus_tag="VMZ53_17630"
     CDS             complement(6289..>7604)
                     /locus_tag="VMZ53_17630"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HUS30336.1"
                     /translation="EDALRCGEMSWSVARELTRVATPETIDAWIERARGRRLRDVEQM
                     VSGRKKGDRPGDPADPARVRQRVVLELDGESLAIWRQMQQVVELECEHKLDDRAFVLA
                     LGSRVLDGELVAANGNREVKAANSNVDVSAGDHALEPENDNDGENDGENDGENDGDSD
                     SDSDSDSDSDSDNDNVGNANTSASTSTLRSHLARHMIHVTTCRDCRRGWQDGAGVRVE
                     LDRSAIDRAECNAIICNDDEGKRPTRTVPARTERLVLERAQHRCEVPGCRSSKHLAIH
                     HIVFRSDGGTHDPSNLVVLCDGHHRVLHDGHLRITGKAPDELVFERAGDALLLGREDH
                     APRKHAARKHPARSLVKRAMTPPDLRNDDPIALAEAALRQLGFKAEVAARAVGAACAH
                     VGTEVELGVLIKEALRRCGDAAMRRRGDAAMRQCGDAAMNDVARP"
BASE COUNT         1215 a         2495 c         2509 g         1385 t
ORIGIN      
        1 aaggcgaagg cggagggaga ggcggaggcg aaggcggagg cgaaggcgga ggcgaaggcg
       61 gaggcgaagg cggaggcgaa ggcggagcga gaggcgaagg cgaaggcgga gggagaggcg
      121 aaggcggcgg cagagggaga ggcgaaggcg gcggcggagc gggacgcgga ggcggaggcg
      181 aacgcgaggg cggcggacgc gtgcggaagg tgcgccgacg cgggctctgg gaccatcagg
      241 atttcttgcg cctatggtcg gcgcaggcga tcagcgcaat cggttcgcgc atcacgcgaa
      301 cggcgctccc gatcatcgcg atctcgacgc tccaccagcc ggagacatcg gtcgcgctgc
      361 tgtggtcgct ctacatcatt ccggggctgg tgctcgcgct ctttgcaggc ggctttgtcg
      421 atcgcacgcg caagcgtcgc attctcattg ccgcagatct gtttcgcgcc gcgtgcgtcg
      481 gttcgctgac gctgagctgg gcgctcgacc tgctcacgat ggcgcagttg atcgtggtcg
      541 gcatgttcgt gggcgcggca tcggcgctgt ttcaaatcgc agacgtcgcg tacctgccga
      601 cgctcgtcag caagaagcag ctgcccgaag gcaacgcgaa gctcgaagcg acggagggcg
      661 tcgcggagat cagcgggcct gcgagcgccg gcatcttgat cggtgtgctc ggtgcgccgc
      721 ttgcggtcgc gctcgatgcg ctcagctacg tgtggtccgc cgcgatgctc ggccgcattc
      781 gcacgaagga gcctgcaccg ctcgcgtcag cgtcgtcggc gttgtcgttg tcggcggtgt
      841 cggcgtcggc gtcggcttca ctcgcgttgt cggcttcggc ggcgtcggcg tcggcgtcgg
      901 ggtcggcgtc ggcgtcactc gcgttgtcgg cgtcgtcggc ggcgtcgggg tcggggtcgg
      961 cggcggggtc ggcggcgtcg ggggcggcgg cgtccgcgcc ggcgggcggt ggctcgacgc
     1021 tcgcgcggac gacactctcg cgtacgcggc gcgatctgag cgttggaatg cgcgcgatct
     1081 ttggtcaccc gcacgtacgg ccgatcgtga tcgcgctgct cgtgtggtcg atcgtcggcg
     1141 gattcttcac gtccctgtat gcactctatt gtttgcggac gctcgatctg tcgcccgagc
     1201 tgttcggcgc gatcgtcgcg atcggtggag cgggctccct tgccggtgca ttcatgtcgc
     1261 ggcgactcgt cgcgcgcatc gggcttgggc ccacgatgat catgtcggcg gtcatcagcg
     1321 tcgtatcggg cattttgatc ccgctcgcgc gcgcgccgga ttggttcgcg ctcgtctgcc
     1381 tcggcgcgca tcagctcatc aacgactgct tctcggtcgt gttcatgatc aacgcgacgt
     1441 cgctgcgaca gaccgtgctt cctcgcgccg tgctcgggcg agcgaatgcg gcattccaca
     1501 tctgcatgtc aggcccgtat ccgatcgtga tcatcgcggc gggcgcgatc gccgaagcga
     1561 catcgattcg cacggctgtg tgggtcggca tgttgatcgg cctcgtcgtg ccgctgctgc
     1621 tgctctcgct gcgaagcttg cggaagatgc ccgaggccgc tgcgtcatcg accgatctgc
     1681 ccgttgaagc agcttcttcg gcgtcggaca tgaccttgcc gctcagcacg tagcgcaagt
     1741 tcgagtctcg cgccgagcac ttagctcgct ggcgtggcgg cgcctacatc gcacgacgca
     1801 cgtcatagac ccgccgcatt cggcgcgcgt tgatagagcc agacacgaaa tcgcgacgac
     1861 gaaacgcagc aacgccgctg cctacctcga agtgatcgac cccgcgcctg gaaagcgctc
     1921 gcgtgtccaa aggaactgcc atgtctcgcc ttacgactct ctcttgttcg cttcttgtcg
     1981 ctgtctcgtc tctcctcccc gccgccgccg tcgcgaagcg catgaacccc aatgcgtacg
     2041 actcggtgat ggcgcctgct gaacagccgg tcgctccatc gaagccgatc catggtggcg
     2101 gcgcgccgtc caacggcggc ttcgcgcaac ctccgcctcc gctctccgcg ggtgccgcag
     2161 cgcaggcgga catttacgag ccgtcggtcg cgttcgcgcc acgtcgctcg ctcgatcgcg
     2221 caacggtccg cgctgcgctc gtacaagcgc gctcgcgcaa cctcagcgcc ttccgcgact
     2281 atcagaagaa gggcgtgttc ccgagcaact cgttcaaggg caagaagctc aacgtctggc
     2341 tcgacgccga tggtcacttc tgcgcggccg cgacgatcat caagacctcg ggccaagatg
     2401 acctcgtgaa caaggtcgcc gagcagaaca acttcatccg tcttgccgac gtgaagcagg
     2461 gcccgctcat ggactggatc ctgacctcgg gcttcaccca ggacgagatc gccgcgatcc
     2521 aagagccgtt catgccggtc gtcgatcggc ccgcgctcga gcccgcaaag ccgatcctcg
     2581 tcgatgcgaa gctccgcaag gcggaagacg cgcgcctgat ggcgaagtac aagcaggtcg
     2641 acaagcagct cgtgaagaac gcgagcaaga gcctcgacaa ggcgaccgat cgcctgatga
     2701 agaacccgac gctcgcgtgg cagctcatcg ccagcaccga cgtcgacgtc ctcgagtagc
     2761 tgtcgtacgc gcttggtgat cggccgtcgt tagagcctga cctgcaaagt cggactctaa
     2821 ggatcgcgaa aacggtcccc gcactcgtta gagcctgacc tgcaaagtcg ggctctcggg
     2881 cgtttctacg cgacggagat ttcgcgcacg acggcgtcga ggacttcggc accccacgcg
     2941 aggcccgcga cgggcactcg ttcgtcgtgg ccgtgaaaca tctcggcgaa cttgatgccc
     3001 gagctcttct cgatcttgac cggcgagaag ccgtaccagc gcgcgcccat cagcgtgaag
     3061 tacttcgcgt cggtgaagcc ggggatcaag tacggcacca cttcggcatc gggctcgcgc
     3121 gattcgacct ggcgcttgat gatgtcgaag agcggcgacg ccatcggctc ggtcacgatc
     3181 ggcggcgcgg acttcatcac ttcgagctcg acctccggcc caagcaccgc acgcagctcg
     3241 cgcagcagat cctcgtcggt ctgcccgggc agcgtgcgcc catcgatctc gaactcggcg
     3301 acgccgggaa tcacgttggt cttcgcgccc gcgcgcacca cggtcgcgga cgcggtgttg
     3361 ctcagcagcg cgagaaagct gcgcgagatc gacgcgcccg gcacgagtcg cgcgatgcgg
     3421 ccgagcagct gaggccgcgc gacgagcttc atgaacggct gcacgaccgc cggctgccgc
     3481 gcacgcaatg catcgaggaa gtccgtcaga tacttggtcg gatgcgtcgg caggctcgtc
     3541 tcgccgatgc gcgtgagcgc ttcgcccaac ttcgtgatga cgctgtcgtg tcgcggcatg
     3601 gagccgtgac cgggctcgcc cgtgatccgc gcgcgcaccc agcagaagcc cttctccgcg
     3661 acctgcacgg gatagaacgt cgactcacca agatgtagcg agaagccgcc cgactcgccg
     3721 atcgcgtact ccgcctcgac cttcgacttg tggcgctcga cgagaaaccg cgagccgagg
     3781 tcgcagcccg cctcttcgtc ggcgacggcc gcaaagatga tgtcgcgatc gaggcgcgtc
     3841 ttcgtcgcgg ccaagcgccg cagaatcgca gtgcacatcg cggccatgtt cttcatgtcg
     3901 atcgcgccgc ggccccacag gcagccctcg aactcgtcgc cggaaaaggg accgcgccgc
     3961 cacttcgacg gatcggcctc gacgacatcg aggtgtgccg tcagcagcaa gggcggcagc
     4021 ttgcccgtcc ccttgtagcg aacgaccaag ttcgtgcggt gcggttccgc ctcgagcagc
     4081 tcgggcacca gatcgacttc gcgcagcgcg ttcgcgatca cttcggccgc cgcacgctcg
     4141 ttgcccggcg gattcgtcgt gtcgatgcgc agcagtgact gacaaagccg tctcgcgtcc
     4201 tcgccgaggc tcggcaacgc agccgctgct gcagcggcag ctccgctgct cgtatcgata
     4261 cgcgacgtgc tgctcaccga ccgaatctac catcgcctcc cggccggcct gcgaacgact
     4321 tcttccgcgg ccctaccgca gacctcagga aatggacgat tcggagcaat tcgcgccaaa
     4381 aaccgagcga acaacgacac tggcgcacga ccgcggcacg accagtcgtc gacggtgcga
     4441 cgactcgagt cgaggcctga ccgatgcagc tgaagcgagg aagctgcatc actcggggga
     4501 gggtgatggc agttcgagag accacacaga tccctcgaag ggtcagaccc cgaatcgaat
     4561 cgttgcttcg aatgtcaaag agctgctgcg tgcgttgacg aggtgctcgc gtcaccgcac
     4621 gtcgcacgaa caaggccgaa cgacgtgcgg cgacaacgag cgaaagactc atcgtgccca
     4681 tgtgggcatt cgttcgctcg cgtcaccgca cgtcgcacga acagggccga acaacatgcg
     4741 gcgacaacga gcgaaagact catcgtgccc acgtgggcat ttgttcgctc gcgtcaccgc
     4801 acgtcgcacg aacagggccg aacgacatgc ggcggtcgtc gcgcccacgt gggcatgggt
     4861 tgtggtcggt cgtcgtgccg cgcattgatc gtcgcgcgtc gagctcacgc gggcatggct
     4921 tgccagttgt ggttccaatg tgggcgcttg ttcgcgccga gctcggccga ggcttcagtg
     4981 gagagttgtc tcgcctttga cgtcgaggaa tggctcgagg cgtttgaacg tcttgtagcc
     5041 gaagcctttg acgcggcgga tgtcggcgac gcggcggaat ccgccgttct ttttgcgcca
     5101 cgtgacgatg cggtcggctt tcgccgggcc gactgtcggt agtcgcatca gctcgccctc
     5161 gttcgcggtg ttcaggttga tcttgcctac gaggtctttg gcgggcttct tgattgggcg
     5221 ttcgggcggt tcgtcgacgg acgactcgtc ctgcgcgctt gcctcggcac tcgttgcgtg
     5281 ggcgcttgcc tgcgcgcgtg gctttgcgga tggcgtcttg cctgcatcac gagtgggtgg
     5341 cccggctttc gccgtcgtcg gctgggcgga cgccacgcgc catcggccgt ccatcgagac
     5401 gacgagcaac atgagcgcga gcaccggaac gcagtaccgg gctagctgac gcaatcgatg
     5461 ctgcgcgtag ctgcgaagcg gcgcgtcgaa gacgtccggt tcgtgctcgt cgagatcgat
     5521 gacgtcggga tggtcggggt ttcgttctgg tgtcgcgatc atgttcgtgg ctcccgcgtg
     5581 tgatcgatca gaacggcggc tgatcgttgt tgttgtcttg ggcgttgagc gcgttgaggt
     5641 gatcgaggcc acccgcaccc gggatcatcg ggatgtggtt cggatcgggc ggatcgaacg
     5701 tcgacggacg gcccagcgtg ccccgcgcgg aatacgacga cgacgacgac gacgacgacg
     5761 acgcgccgcc gcgatcggag tagccgcgat cggagtagcc gcgatcggag tagccgcggt
     5821 ccgagccgcc gccgccgcga tcaccgctcg aggcgcgctt ctcgctgcgc tcgcgcattt
     5881 cctcttcggt gacctcgcga agttggagcg tcagcgggcg gccatccttg ttggcgttca
     5941 ctggcagcgc atcgatgtac atgttgatgg actcgtcttt gttgcggtag ccgactccgc
     6001 aacgcatcca gaacttcgat ccatcgcgct tgtcgatggg gctgatgact ttgaacatct
     6061 tcttcttctc ttgaaacacg gcagtctcct tggctgtaac gacgccggat gcgtcgcggg
     6121 cagccagaag agcaagggaa gtgccagggc gagagcgcgc taggttccgg aaatgacatc
     6181 ggccgtgcga cgcacgtgtc cggaaaccgg acgtcgtgtc cgagagccgt tcggacaccg
     6241 gacaaaggcc ttcgaaccgc cacacctcat gacgcggtca cggcggcttc atggccgtgc
     6301 aacatcattc atcgccgcat cgccgcattg ccgcatcgcc gcatcgccgc gtcgccgcat
     6361 cgccgcatcg ccgcatcgcc gcaacgcctc cttgatgagg acgccgagct cgacctccgt
     6421 gcccacgtgg gcgcaagccg ccccgaccgc acgcgccgca acctccgcct tgaacccgag
     6481 ctgccgaaga gcagcttctg cgagcgcgat cggatcgtcg ttgcgaaggt ccgggggcgt
     6541 catcgcgcgc ttcaccaagc tgcgtgcggg gtgcttgcgt gcagcatgct tgcgcggcgc
     6601 atgatcctcg cggccgagga gcagcgcgtc gcccgcgcgt tcgaacacga gctcatcggg
     6661 ggccttgccc gtgatccgca gatgcccgtc gtgcaacacg cgatggtgcc cgtcgcacag
     6721 tacgacgagg ttcgatgggt catgcgtgcc gccgtccgag cgaaacacga tgtgatggat
     6781 cgctagatgc ttcgatgacc gacagcccgg cacctcgcac cgatgttgcg cgcgctcgag
     6841 caccaggcgt tctgttcgcg caggaaccgt acgcgtcggt ctcttccctt cgtcgtcgtt
     6901 gcaaatgatg gcattgcact cggcgcgatc gattgccgat cgatcgagct cgacgcgcac
     6961 gcctgcacca tcctgccagc ctcgccggca gtcgcgacat gtcgtcacgt gaatcatgtg
     7021 ccgagcgagg tggctgcgaa gcgtgctcgt cgatgcgctg gtgtttgcat tcccgacgtt
     7081 gtcgttgtcg ctgtcgctgt cgctgtcgct gtcgctgtcg ctgtcgctgt cgccgtcgtt
     7141 ctcgccgtcg ttctcgccgt cgttctcgcc gtcgttgtcg ttctccggct ccagcgcatg
     7201 gtcgcccgca ctcacgtcga cgttgctgtt cgcggccttc acttcacggt tgccgtttgc
     7261 tgctaccagt tcgccgtcga gcacacgact accgagcgca agaacgaacg cgcggtcatc
     7321 cagcttatgc tcgcactcga gctcgacgac ctgctgcatc tggcgccaga tcgccaagct
     7381 ctcgccgtcg agctcgagca cgacgcgctg tctcacacgc gcaggatcgg cgggatcgcc
     7441 cggcctatcg cccttcttgc ggcccgacac catctgctcg acgtcacgca gcctgcgccc
     7501 acgtgcgcgt tcgatccaag catcgatcgt ttcgggcgtc gccacgcgtg tgagctcgcg
     7561 tgcgacagac cagctcatct cgccgcatcg cagcgcgtct tcga
//
LOCUS       DATLBZ010000342        15162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9889249, whole genome shotgun sequence.
ACCESSION   DATLBZ010000342 DATLBZ010000000
VERSION     DATLBZ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15162
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9889249"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            607..1800
                     /locus_tag="VMZ53_17635"
     CDS             607..1800
                     /locus_tag="VMZ53_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30337.1"
                     /translation="MRRFLLCVAVTTMACGPAIPPVPGKGGPAWHELTSEHFTVWTDA
                     DESTGRELVAHMEDLRQAVIGIGFRGAARPGRSLVIALRDDDEVSAFLPRGDIVAIAS
                     SGGVIHQPMILLSANPTHDPKGNITAHELTHTISRAVIRNQPRWFAEGLAEFFATIEI
                     DRGAGTIDLGRAPTYRGVPVHMRRMIPLAKLFGCKTFACIDGNFYVTAWAFFTFAFNR
                     YPQQLAQLEDLLASGSDESVAWATAFGAVPVETLDAEMRQWIAHGTHTVMHFNAQLKS
                     FGVSVRSLADADVYAARALLRLQYQENRAEVRRLLDAAIALDRTHPLARLLEYELEDK
                     VDLDVARAVTDAHPGDWRGWFLVEEATRDREAYDKMCAAIAATNPLIEVGCAPVVTSQ
                     SPGPS"
     gene            complement(1772..4027)
                     /locus_tag="VMZ53_17640"
     CDS             complement(1772..4027)
                     /locus_tag="VMZ53_17640"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HUS30338.1"
                     /translation="MRHLGVLLVVFLCACGDNIKQTPQDAGADGPELKCGNGVLDGNE
                     ECDDGEENGITGARCDTTCKWVCLEDLKCSNMDPCDGEETCVDHRCVAGTDVVDGTTC
                     GTGKLCRNAVCTDAVCGDQFVTGPAEECDDANSTQGDGCDNNCKLSCVSTDSTRNCTP
                     ADACAGQGTCNDSTHACTAGTPLANNTACGTGGYCKAGVCTQPVCGNGMIEPGEVCDD
                     GTSNGQPGDGCKSNCQYVCVNAAADCPAAAFCQMNTCTGSHTCAAVADPAKENMTCGS
                     NLVCKSGACTALTAVCGNGTVETGEQCDFGGSNGAGTGCETSCQFSCQTTPTDTCNDS
                     NACNGTETCGNVTVNARTGKRCSSTAPLTNGTSCGTNQICLSQVCVTSSCGDSFVDSN
                     RGETCDPPGGTCDASCHAIACGDGVRAGTEQCDDSNTTNLDGCDSSCKFEQCHRVNSL
                     SIAFPPSNIDAPFCAANALGGAVVGGTAQNQLGAALTSGVRDGSITIELKALGLTDLT
                     GTSGNFTLGALTGTPVTGTSTYNGTSDLDWWYTTAANVIDGSRNPTTTIPASITAKNL
                     TTTPTEIVISVSLGGQPATLDMLNAKLKGNIGAANTPLTSTGGPPGHLASEHLDPTLT
                     SFATVSAGELCGDVTAQSLYAVPIPTALVGCSLVSCSACYTASNTLLDVIVNGCNTLI
                     GQQIKGTQPDKSRVTGTTYSFVYDNTTKTITGCTKKVGMTTSSATLTECRQDSAYSTL
                     LRFTTDRVIAK"
     gene            4145..5779
                     /locus_tag="VMZ53_17645"
     CDS             4145..5779
                     /locus_tag="VMZ53_17645"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HUS30339.1"
                     /translation="MLRGALCIGLVVALAPACESEPPDPYLPLLEKHAGHTDPPPDTS
                     NKYALDPAAQALGKKLYFDPNFAGVVIGKDMLLRDMTTAPREPLGASAHVSCNSCHDV
                     TMGGSDHTTTPGNVVSFGAGAYDVNSQQTINSAYADIIYWNGRNDSLWSQIMAVVESH
                     VSVNSNRLRIAWRLIDKYRSEYAAVFPEWPLPPELDDLAMQKSRLNPDGTCVLDGSNA
                     CPAYCHNTYGPCLPRYPLDGRQGYVELGELPHCDWADPALDPVLQPYKDAWDCMKLED
                     QLLATRIFVNFAKSIAAYEFTLISKDSPFDKWADAGFPAGMLSPAAERGARLFVGKAA
                     CAECHEGPMFSDWKFHAIGVPQLGTYVPTKADCPAGGWCDCVTNDSALPENCLPNGAR
                     DGLRKLRANRFRRDSAWSDDEECRRHFTLHVDANYNAAHPTECDGKVKYYGLTFDDSM
                     VGQWRTPSLRDVAITAPYMHDGVYANLRDVIVHYNKGGVHSLGGETLGTIDEKIKVLN
                     LTDQEIDDVVAFLETLTGQVDPAVIEMPVVPPASAF"
     gene            5795..7018
                     /locus_tag="VMZ53_17650"
     CDS             5795..7018
                     /locus_tag="VMZ53_17650"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS30340.1"
                     /translation="MMRAWQEFVVVVALVAACGDNKDRPAPDAAPDAGGQGQVERGQY
                     VMNVLANCTFCHTPLRADGTRDTDRLLAGVDCFADFDSPTFTDNLNGVGCISTRNLTP
                     HSTGLAGKTDEQIKNAFRNGVRTDGKKLAPLMPYWIFHNMSDEDADAVVAYLRTVPPV
                     DHTVKPNEFPWGCGGTPPPIPGATCGAPGPLATYNDGTIPQPPFLSAVTIPMPRGGAN
                     NQSAMRGRYLSSMAGLCVDCHTPTLSEDPMDVVGILTADQTKFYGGGRLFPKGALGLV
                     APGFNYPAFVVTRNLTSDATGLGGWSRDQIKNAIAIGKDRDGKAVCAATHGGVISPYA
                     ALTDQDLTDIVEYIYNLPPVENDTATKFCPAPPIPNPAGAETGLQCGNGTDDDGDTVP
                     DDGCACGNCQGPPVQ"
     gene            7028..7975
                     /locus_tag="VMZ53_17655"
     CDS             7028..7975
                     /locus_tag="VMZ53_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30341.1"
                     /translation="MKRSIVVLVAGTLGACSDPPTMMPDAETLPSTMPERLSLTGIYT
                     DIATKALNPKLVPFAPNNVLWSDGAVKTRWYQLPDGAVIDSSDMDHWKFPVGAKFFKE
                     FALDGKRLETRLIWRVADTGNFERDTLVGAFLWNDDESDAVFAKDGGQNLRGTDHDAP
                     AADTCWKCHAGEAGHVLGLSALQTGDVSKLPLSSPPPAATTYAAPNAALGYLHANCGH
                     CHNPQGGGWQDSHMVLRLDVDERDAATTKIVQTIVDQDLETWLNHGYAKRVVPGDPDA
                     SALFYRMSQRTMMVQMPPLATEHTDDAGLALVRAWIQSL"
     gene            7972..9384
                     /locus_tag="VMZ53_17660"
     CDS             7972..9384
                     /locus_tag="VMZ53_17660"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUS30342.1"
                     /translation="MRRLAYLLVLLASPAFAQDAPVPVDPPQPAPEPAPEQQDPPARP
                     DDPKLKELEDRLQAVEDELDAQKDDNKYLEEKIESLVPLKITGYLDLGAFATTGNGAG
                     TRTDVGHLLFPEYSYVPETWVFYGDPLSTAVNSRGDVADTGESRAIVFDPINSNGKPT
                     ALVNAVNLALFSGIGETGQINVSVDLVPRSRNISDPSGTFLGDFLDVKLAYGEWRPHV
                     SEAGTWDLTLQAGKFDSVLGYEYRSIESPDRVGVTPSLICRYTCGRPIGLKARARFLD
                     EALIANVAVTNGSHFSEGFDFANETDTNSMKTVAGRLSFSIQKKVELGASGAWGAQDF
                     QPDNDVRQWHIGGDVHAEIKDFELTAELVHGRAKGKTSMVRCDAAPCLRYTGGYLQMA
                     YRVTNTWVPYVRADYRDADHWSGASFVYVSDLWRATVGIRAEIGTRIIWKLEVTKNNE
                     LGIPYTIPNDVATTSLVIKY"
     gene            9394..10044
                     /locus_tag="VMZ53_17665"
     CDS             9394..10044
                     /locus_tag="VMZ53_17665"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30343.1"
                     /translation="MRTVVILATLVLFIGDAGSKPQTGSVSGTITLKGAKGTQKPVVV
                     YLEDVAGTPAVPKDHAIIRQREKQFEPPLTVVVRGTTIDFPNEDKIFHNVFSVSRPAR
                     FDLGLYKSGTAKSVEFKRAGVVDVYCNIHPEMIAKVKILDNGYYTISDGKGAFRIDGV
                     PEGTYPVVAWLPSGDEAKGSVTVKAGASAEVKLELSETVKKETHARKDGTPYGRYK"
     gene            10179..11159
                     /locus_tag="VMZ53_17670"
     CDS             10179..11159
                     /locus_tag="VMZ53_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HUS30344.1"
                     /translation="MFVTGSGRCGECHEKMFDEWESSAHAQAVSSELYKKSVAAAKDV
                     TCERCHAPLASEVPRDAIVTEGVTCDVCHTLREPKPSVDGGSWKLAIDDMVKFGPRCD
                     LKDHYFHRMGCSPEHKTAEICGTCHWWEPKGLPVFTEYADWKAGPKHDTPCQDCHMPK
                     SRAALATGSPVRDGVPHHGLLGKAKDLRKTSLHMDAKLAPDGALTVMLENKHAAHYVP
                     SGLPERRIVVRARLLDASGAEAWHDQRELGRMLVDANGKEAPFWAATRVGSDTRIAPA
                     GQSTLTFAVPERKGRVEIVVLYRGLSEAIAKQLGVSEVEEQKLLEKIVKK"
     gene            11156..12955
                     /locus_tag="VMZ53_17675"
     CDS             11156..12955
                     /locus_tag="VMZ53_17675"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HUS30345.1"
                     /translation="MKLRTTVFLWVLLLVVVVLGATIGTIVFVFNNSTRERVADELVR
                     SRDVTLDLHATRDSLHRQECRVVADEPRLRAVVATEDVARETIVDAVRTLADTLHAGV
                     FVIVDAEGNLIADNAVPDAVGFSLMNRPEVAAALEKGEGTGIWLADDRVYQVQACRLE
                     FGARVVGALVVGHAIDNELANVVARQTGGWLVVVEDKLPRTEPPIGTKPGEVGAVVQA
                     VRAGDKEVSLGDTHYFAQLVPVPGYKGEHKVQYLLLRSIDEALAPGKKIVRILMGLLG
                     IAVLATLVLALGLARRLSRPIDSLVTRTLAIAQGDLRARPVSGPTEVKALGSAMDRMV
                     KEIDESRRSLADKERLAREMEIAARIQTSILPRNIAVDGLEITARMMTATEVGGDYYD
                     VLTVDGGCWIAVGDASGHGLTAGLVMMMVQTGVATLVRAKPDANPKDVLKTLNRVLFE
                     NVHDRLEAERHMTLCLLRYRGGGKLTVAGAHMDAVVWRAASQTVEMLTTKGTFLAITD
                     DIDHVNVEQEWSLAHGDLLVLLTDGVTEAENAAGTPFGYEGVVEVVEPRATKPVVAIQ
                     NALFDAVTKHSPTLADDCTILVLRYVGPGETDG"
     gene            12955..>15162
                     /locus_tag="VMZ53_17680"
     CDS             12955..>15162
                     /locus_tag="VMZ53_17680"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30346.1"
                     /translation="MAELHINVGMVALSRGFITLEAFAKGIEKLAQSERQSIHELWGN
                     HMSDDQLAMVLDVIGKVDSKAPDTMIVPTNVKAIVPPTPGALSTKVGRKQSPTGPGTA
                     PTAASIVELPGESKAPPVPRTITPNMTIPMPMADLLGARYKKLGVLGKGGLGEVLQCD
                     DPVLQRTVALKAGHVKGDVDAYSTKVILAREARIIAGLEHPNIIPIYDAGDDPLRGPY
                     YVMRQVTDTSLEAILHQRKKGEGNPHDYTLNRLLRYFLQVCNAVDYAHHRGVIHCDIK
                     PANILLGDYGEVLLVDWGLAQSRNHPLGVRGGTLGYMAPEQMDPSIERLDWRTDVFAL
                     GAIIYEILCGVPAFEEARSSDLTAIGKDPKRVYKLPLAPSQRDSRLEIPKEVEDVCMQ
                     AIAVEREKRIETVRELANAIEDWIEGTKQKERQRIEADKSADAGDELAERYHEFVESR
                     PEKMTVFRALRADVAPWADLDEKQDLWDAEDIVKVTDALQVRTFHSAVSAYERALDQV
                     PNHTRARRGLARLYWSELQRARHRGETLDQVAYEQLLREVDDGTFMRELQRDGLLELT
                     FGAHAGKVTLAPLVEKQRRLVEGVVEVIEERPMAHRSLAAGRYVVATSIGDGPHRVMW
                     PVNIEPGALCKISVDPPQLCRASEDEVLVPEGIARLGGDPFSSDADELVTVDVPSFVI
                     QRFPVTFARWYEFIDDLKRTGIPFEAHVPRTPIGLPPEPQRLPVFGVSAESAEA"
BASE COUNT         2687 a         4779 c         5059 g         2637 t
ORIGIN      
        1 cgtacccgcg tgggcatgga atcgcgacaa cgacgtggtc gtgcccgggt gggcactgtc
       61 gcgacgacga ccaacttcgt cgcgcccgcg tgggcacggt tgcgagaatg gccgacgggg
      121 cggtcgtgcc cacgtgggca cggaggcgcg ggttgatcgc gcgcatgcgc gtgctgcggc
      181 acggcccgcg gcgtcgacgg caacacgcgg tcgcgcccac gtgggtactg tcgcgacgac
      241 gaccgacttc gtcacgccca cgtgggcacg gttacgacaa ctatcctggc gcatagggcg
      301 acgaccgacg tgtcgcgccc acgtggtgcg cggagtcgcg acgacaaccg acttcatcgt
      361 gcccacgtgg gcactgtcgc gacgacgacg gtcacggtgg tcgtgcccac gtcggaacgg
      421 aggcgcgggt tgaccgcgcg aggcgcgtgc tgcgtctcgc gacgacccgc cgcgatgacc
      481 gcaccgcacg accgctcacg tcggcacgga atcgcgacga cgacggtcgg ggtggtcgtg
      541 cccacgtggg cacggaatcg cgagcacggc gccggttgcg gcgtcgcggt atggtcgttg
      601 tcgcggatgc ggcgtttcct gctttgcgtt gcggtcacga caatggcctg cggccccgcg
      661 atacctccgg tccccggcaa gggcgggccc gcgtggcacg agctcacgag cgagcacttc
      721 accgtctgga ccgacgctga tgagagcacg ggccgcgagc tcgtcgcgca tatggaggac
      781 cttcgccaag ctgtgatcgg catcggcttt cgtggtgccg cgcggcccgg gcgctcgctc
      841 gttatcgctt tgcgcgacga cgacgaagtc agcgcgtttc tgccacgcgg cgatatcgtc
      901 gccattgcga gctcgggtgg cgtgattcat cagccgatga tcctgttgtc ggcgaatccg
      961 acgcatgatc cgaaggggaa catcacggcg cacgagctca cgcacaccat ctcgcgcgcg
     1021 gtgatccgca atcagccgcg gtggttcgcg gaagggttgg ctgagttttt tgcgacgatc
     1081 gagatcgatc gcggcgcggg aacgatcgac ttgggtcggg cgcccacgta ccgcggcgtg
     1141 cccgttcata tgcggcgcat gatcccgctt gcgaagctgt ttggctgcaa gacgttcgcg
     1201 tgcatcgatg ggaactttta tgtgacggcg tgggcgttct ttacgttcgc gttcaatcgc
     1261 tatccgcaac agctcgcgca gctcgaagac ttgctggcgt cgggcagcga tgagtcggtg
     1321 gcgtgggcga cggcgttcgg ggcggtgccc gttgagacgc tcgatgcgga gatgcggcag
     1381 tggatcgcgc atggcacgca cacggtgatg cacttcaatg cgcagctgaa gtcgtttggc
     1441 gtcagcgtgc gatcgctggc ggatgcggac gtgtatgcgg cgcgggcgtt gctgcgcttg
     1501 cagtatcagg agaatcgggc ggaagtgcgg aggctgctcg atgcggcgat tgcgctcgac
     1561 cgtacgcatc cactcgcgcg gttattggag tacgagctcg aggacaaggt cgacttggac
     1621 gttgcgcgag ccgtgacgga cgcgcatccg ggtgattggc gcggctggtt tcttgtggag
     1681 gaggcgacgc gcgaccgcga ggcgtacgac aagatgtgcg cagcgattgc ggcgacgaat
     1741 ccgctgatcg aggtcgggtg cgcgcccgtc gttacttcgc aatcacccgg tccgtcgtga
     1801 acctgagcag ggtggagtag gcgctgtctt ggcggcactc cgtgagcgtc gctgacgagg
     1861 tcgtcattcc gaccttcttc gtgcagccgg tgatcgtctt cgtcgtgttg tcgtagacga
     1921 acgagtaggt ggtgccggtg acgcgcgact tgtccggctg cgtgcccttg atctgctgac
     1981 cgatgagcgt gttgcagccg ttgacgatca cgtcgagcag cgtgttggac gccgtgtagc
     2041 acgcactgca gctcacgagg ctgcagccga cgagcgccgt cggaatcggg acggcgtaga
     2101 gcgactgcgc cgtgacatcg ccgcagagct cgcccgcgga gaccgtggcg aacgatgtca
     2161 gcgttggatc gaggtgctcg gaggcgaggt ggcctggtgg gccgcccgtc gaggtgagcg
     2221 gcgtgttcgc cgcaccgatg ttgcccttga gcttcgcgtt cagcatgtcg agcgtcgctg
     2281 gttggccgcc gaggctgacc gagatcacga tctccgtcgg tgttgtcgtg aggttcttcg
     2341 cggtgatcga tgcggggatg gtggtcgtcg gattacggga gccgtcgatc acgttcgcgg
     2401 cggtcgtgta ccaccagtcg aggtctgacg ttccgttgta cgtcgacgtg cccgtcacgg
     2461 gcgttcccgt gagcgcgccg agcgtgaagt tgcccgacgt gcccgtgaga tccgtgagcc
     2521 cgagcgcctt gagctcgatc gtgatggagc catcgcggac gccgctggtc agcgcggcgc
     2581 cgagctgatt ctgtgccgtg ccgccgacga ccgcgccacc gagcgcattt gccgcgcaga
     2641 acggggcatc gatgttgctc ggcgggaacg cgatcgacag cgagttgacg cggtggcact
     2701 gctcgaactt gcagctcgag tcgcagccat cgaggttcgt cgtgttcgag tcgtcgcatt
     2761 gctcggtgcc tgcacgcacg ccgtcgccgc acgcgatcgc gtggcagctt gcatcgcagg
     2821 ttccgcccgg cggatcgcag gtctcgccgc ggttcgagtc gacgaagctg tcaccgcacg
     2881 atgacgtgac gcacacctgc gagaggcaga tttggttcgt gccgcacgaa gtgccgttcg
     2941 tcagtggcgc ggtcgacgag cacctcttgc cggttcgggc gttcaccgtc acgttgccgc
     3001 atgtctcggt gccgttgcac gcgttgctgt cgttgcatgt gtcggtcggc gtcgtctgac
     3061 acgagaactg acacgacgtc tcgcatccag tgcctgcgcc gttgcttccg ccgaagtcgc
     3121 attgctcgcc ggtctcgacg gtgccgttgc cgcagacggc tgttagcgcc gtgcacgcgc
     3181 cgcttttaca gacgaggttc gagccgcacg tcatgttctc cttcgcggga tcggcgacgg
     3241 ctgcgcaggt gtgcgagccc gtgcacgtat tcatctgaca gaacgctgca gcggggcagt
     3301 ccgcagcggc gttgacgcag acgtattggc aattcgattt gcagccgtcg ccgggttgac
     3361 cgttcgacgt gccgtcgtcg cagacctcgc cgggctcgat catgccgttg ccgcacacgg
     3421 gttgcgtgca gacacccgcc ttgcagtagc cgccggttcc gcatgcggtg ttgttcgcga
     3481 gcggcgtgcc cgcggtgcag gcatgcgtcg agtcgttgca cgtgccttgt ccggcacacg
     3541 cgtcggcggg cgtgcagttg cgcgtcgaat cggtcgagac gcacgagagc ttgcagttgt
     3601 tgtcgcagcc atcgccttgc gtcgagttcg cgtcgtcgca ctcttcggcc gggcccgtga
     3661 cgaattggtc accgcaaact gcatccgtgc agactgcgtt gcgacagagc ttgcccgtgc
     3721 cgcacgtcgt gccgtcgacg acgtccgtgc cggcgacgca tcggtgatcg acgcacgtct
     3781 cctcgccgtc gcagggatcc atgttgctgc acttgaggtc ttcgaggcag acccacttgc
     3841 aggtcgtgtc acaccgcgcg ccggtgatgc cgttctcctc gccgtcgtca cactcttcgt
     3901 taccgtcgag gacgccgttg ccgcacttga gctcgggccc gtccgcgcca gcgtcttgcg
     3961 gggtctgttt gatgttgtcg ccgcacgcac ataggaagac gacaagaagg accccgagat
     4021 gcctcatcgg ccaaggatat ccgcgttcct cgcgcctctc gtgtgctgct tttcgcactg
     4081 ccgagatcgc aagcgtttcg agccgttgac cagtgagctg cggctgtgga acgatgccga
     4141 gatagtgctg cggggtgcac tctgtatagg gctcgttgta gccctggcgc cggcctgcga
     4201 gtcggagccg ccggatccgt atctgccgtt gctcgagaag cacgcgggcc ataccgatcc
     4261 acctccggac acgtcgaaca agtacgcgct cgatccggcg gcacaggcgc tcggcaagaa
     4321 gctctacttc gatccgaatt tcgcgggcgt cgtgatcggc aaggacatgc tcctgcgcga
     4381 catgacgacc gcaccgcgcg agccgctcgg cgcatccgcg catgtctcgt gtaacagctg
     4441 ccacgacgtt acgatgggcg gctcggatca caccacgact ccgggcaacg tcgtgtcgtt
     4501 cggcgcaggc gcatacgacg tcaacagcca gcagacgatc aactccgcgt acgccgacat
     4561 catctactgg aacggtcgca acgactcgct gtggtcgcag atcatggccg tcgtcgagag
     4621 ccacgtctcg gtgaactcga accgtctgcg catcgcctgg cgcctcatcg acaaatatcg
     4681 gagcgagtac gcagcggtgt tcccggagtg gccgctgcca ccggagctcg atgatctcgc
     4741 gatgcagaag tcgcggctca atccagacgg cacgtgcgtg ctcgacggaa gcaatgcatg
     4801 cccggcgtac tgccacaaca cgtacgggcc gtgcttgccg cgctatccac tcgacggacg
     4861 gcagggctac gtcgagctgg gcgagctccc gcattgcgac tgggccgacc ccgcactcga
     4921 tccggtcctg cagccgtaca aagacgcgtg ggactgcatg aagctcgagg atcagctcct
     4981 cgcgacgcgc atcttcgtga acttcgcgaa gtcgatcgcg gcgtacgagt tcacgctcat
     5041 ctcgaaagac tcgccgttcg acaagtgggc cgatgcgggc ttcccagcgg gcatgctgtc
     5101 gcctgcagcc gagcgcggtg cgcgcctctt cgtcggcaaa gcggcgtgcg cggagtgcca
     5161 cgaaggcccg atgttcagcg attggaagtt tcacgcgatc ggcgtgccgc agctcggaac
     5221 gtacgtgccg acgaaggccg actgtcctgc gggcggttgg tgcgactgcg tgacgaacga
     5281 cagcgcgctg cccgagaact gcttgccgaa cggcgcgcgg gatggtctgc gcaagctgcg
     5341 cgcgaatcgc ttccgccgcg actcggcctg gagcgacgac gaggagtgcc gccgtcactt
     5401 cacgctgcac gtcgacgcga actacaacgc ggcgcatccg acggagtgcg acggcaaggt
     5461 gaagtactac ggcctgacat tcgacgactc gatggttggc cagtggcgca cgccgagcct
     5521 tcgcgatgtc gcgatcacgg cgccgtacat gcacgacggt gtctacgcaa acctgcgcga
     5581 cgtgatcgtt cactacaaca agggtggcgt gcactcgctc ggcggcgaga cgctcggcac
     5641 catcgacgag aagatcaaag tcctgaacct gacggaccag gagatcgacg acgtcgtcgc
     5701 gttcctcgag acgctcacgg gacaggtcga tccggctgtg atcgaaatgc cggtggtgcc
     5761 accggcctct gctttctagt taacgaaggg aatcatgatg cgtgcatggc aggaattcgt
     5821 ggtggtggtg gcgctcgtcg ctgcgtgcgg tgacaacaaa gatcgtcctg caccggatgc
     5881 cgcaccagac gcgggcgggc agggccaagt cgagcgcggc cagtacgtca tgaacgtgct
     5941 cgcgaactgc acgttctgcc acacgccgct gcgtgccgac ggtacgcgcg ataccgatcg
     6001 cctgctcgcg ggcgtcgact gcttcgctga cttcgacagc ccgacgttca cggacaacct
     6061 caacggcgtc ggctgcatct cgacgcgcaa cctgacaccg cactcgaccg gcctcgccgg
     6121 taagaccgac gagcagatca agaatgcatt ccgcaacggc gtgcgcaccg atggcaagaa
     6181 gctcgcgccg ctcatgccgt attggatttt ccacaacatg agcgacgagg atgcggatgc
     6241 ggtcgtcgcg tatctgcgaa ccgtgccgcc ggtcgatcac acggtgaagc cgaacgagtt
     6301 cccgtggggg tgcggtggca cgccgccgcc gattccaggt gctacgtgtg gcgcgccggg
     6361 tccgctcgct acgtacaacg acggcacgat cccgcagccg cccttcctca gcgcggtgac
     6421 cattccgatg ccccgcggcg gcgcgaataa ccagagcgcg atgcgcggcc gctatctgtc
     6481 ttcgatggcg ggtctctgcg tcgattgcca cacaccgacg ctgagcgagg atccgatgga
     6541 cgtcgtcggc attctgactg ccgaccagac gaagttctac ggcggcgggc gcttgttccc
     6601 gaagggcgcg ctcggcctcg tcgcgccggg cttcaactat ccggcgtttg tcgtgactcg
     6661 aaatctcacg tccgatgcaa cggggctcgg cggttggtcg cgcgaccaga tcaagaacgc
     6721 gatcgcgatc ggcaaggatc gcgatggcaa ggcggtctgc gcagcgacgc acggcggcgt
     6781 catctctccg tacgccgcgc tcaccgacca ggacctcacc gacatcgtcg agtacatcta
     6841 caacctgccg cccgtcgaga acgacacggc aacgaagttc tgtcccgcgc cgcccattcc
     6901 caatcccgcc ggggccgaga ccggactgca gtgcggaaat ggtaccgacg atgacggcga
     6961 caccgttcca gacgatggct gcgcatgcgg taactgccag ggaccgcctg ttcaatgatg
     7021 tgatgtcgtg aagcgcagca tcgtcgtcct cgtcgccggg acgttggggg cctgttcgga
     7081 cccgccgaca atgatgcctg atgccgaaac gctgccgtcg acgatgcccg agcggctctc
     7141 gctcaccggc atctacacgg acatcgcgac gaaggccctc aatcccaagc tcgttccgtt
     7201 cgcgccgaac aacgtgctgt ggtccgacgg agccgtgaag acgcgttggt atcagctgcc
     7261 cgacggcgcg gtgatcgaca gcagcgacat ggaccactgg aagtttcccg tcggcgcgaa
     7321 gttcttcaaa gaatttgcac tcgacggcaa gcgactcgag acgcgcctca tctggcgggt
     7381 cgctgatacc ggcaacttcg agcgagacac gctcgtcggt gcgttcctgt ggaatgacga
     7441 cgagagcgac gcggtcttcg cgaaagacgg cgggcagaat ctgcgcggca ccgatcacga
     7501 tgcaccggcc gccgacacgt gctggaagtg tcacgccggc gaggctggtc acgtcctcgg
     7561 gttgtccgcg ctgcagaccg gcgacgtcag caagctgccg ctctcatcgc cgcctcccgc
     7621 ggcaacgacg tacgccgcgc cgaatgccgc actcggctat ctgcacgcga actgcggtca
     7681 ctgccacaac ccgcaaggcg gcggatggca ggactcgcac atggtgctca ggctcgatgt
     7741 cgacgagcgc gatgccgcga ccacgaagat cgtgcagacg atcgtcgatc aggatctcga
     7801 gacgtggctg aaccacggct acgcgaaacg cgttgttcca ggcgatcccg acgcgagcgc
     7861 gctgttctac cgaatgagcc agcgtacgat gatggtccaa atgccgccgc tggcgaccga
     7921 gcacaccgac gatgctggtc tcgcgctggt gcgtgcatgg atccaatcgc tatgaggcgc
     7981 ctcgcttacc tgctcgtgtt gctcgcttct cccgcgtttg cccaggacgc gccggttccc
     8041 gtcgatccac cgcagccagc gcccgagcct gcgcccgagc aacaagaccc gcccgcgagg
     8101 cccgacgatc cgaagctcaa ggagctcgaa gaccggctgc aggccgtcga agacgagctc
     8161 gacgcgcaga aggacgacaa caagtatctc gaggagaaga tcgagtcgct cgttccactg
     8221 aagatcaccg gctatctcga tctcggcgca ttcgcgacga caggaaacgg tgccggtacg
     8281 cgcaccgacg tcggtcatct gttgttcccc gagtactcgt acgttcccga gacgtgggtc
     8341 ttctacggcg atccactgtc gacggcggtc aactcgcgtg gtgacgtagc ggatacgggc
     8401 gagtcgcgag cgatcgtgtt cgatccgatc aacagcaacg gcaagccaac cgcgctcgtc
     8461 aatgcagtga acctcgcgct cttcagtggc atcggcgaga cggggcagat caacgtcagc
     8521 gtcgacctcg tgccgcgctc gcgcaatatc tccgatccgt cgggcacgtt cctcggcgac
     8581 ttcctcgacg tgaagctcgc gtacggcgag tggcggccgc atgtctccga agcgggcacg
     8641 tgggacctca cactacaggc cggcaagttc gatagcgtgc tcggctacga gtaccgatcg
     8701 attgaatctc cggaccgcgt gggcgtcaca ccgtcgctca tctgtcgcta tacgtgcggc
     8761 cggccgattg gcctcaaagc acgcgcgcgg tttctcgacg aggcgttgat cgcaaatgtc
     8821 gccgtcacga acggcagcca cttctcggag ggcttcgact tcgcgaacga gaccgatacc
     8881 aactcgatga agacagtcgc gggccggctc tcgttctcga tccagaagaa ggtcgagctc
     8941 ggcgcgagcg gcgcatgggg cgcacaggac ttccagcccg acaacgacgt tcggcagtgg
     9001 cacatcggtg gcgacgttca tgccgagatc aaagacttcg agctgacggc cgagctggtg
     9061 cacgggcgcg cgaagggcaa gacgtcgatg gtgcgatgcg atgcagcgcc ctgcctgagg
     9121 tacaccggtg gatatctgca aatggcctat cgcgtgacga atacgtgggt tccctacgtg
     9181 cgcgccgact atcgcgatgc cgatcactgg tcgggcgcga gctttgtcta cgtgtcagat
     9241 ctttggaggg cgaccgtcgg cattcgcgcg gagatcggga cgcgcattat ctggaagctc
     9301 gaagtgacga agaacaacga gctcggcatc ccgtacacga ttcccaacga cgtcgcgacc
     9361 acgtccctcg tgatcaaata ctaggtggca cccatgcgaa ctgtcgtgat cctcgcaacg
     9421 ctcgtcctct tcatcggaga tgccggcagc aagccgcaga ccggtagcgt cagcggcacg
     9481 atcacgctca aaggcgcgaa gggcacgcag aagcctgtcg tcgtgtatct cgaggatgtg
     9541 gcgggcacgc cggcagtgcc gaaggaccac gcgatcattc gccagcgcga gaagcagttc
     9601 gagccgccgc tgacggtcgt ggtgcgtggc acgacgatcg actttccgaa cgaggacaaa
     9661 atctttcaca acgtgttctc ggtctcgcga ccggcgcgct tcgacctcgg tctctacaag
     9721 agcggcaccg cgaagtccgt cgagttcaag cgcgcgggcg tcgtcgacgt ctactgcaac
     9781 attcaccccg agatgatcgc gaaggtgaag atcctcgata acggctacta cacgatcagc
     9841 gacggcaaag gcgcgttccg catcgacggc gtacctgaag gaacgtatcc ggtcgtcgcg
     9901 tggctgccgt cgggggacga ggcgaaggga agcgtcacgg tgaaggcggg cgcgagcgcc
     9961 gaggtcaagc tggagctcag cgagaccgtc aagaaagaga cgcatgctcg aaaggacggc
    10021 acaccatacg ggcgctacaa gtaatcgcgc tcgttgctgc tgcctgttcg tcgcagaaac
    10081 aagcgccggc gaagaaagac gatgcgcgcg gtgcgatcgt cgcgccgccc gttgctgatg
    10141 cagcggccgc caatgcgatc gatgcgaaca cgcgaaaggt gtttgtcacc ggctccggtc
    10201 gctgtggcga gtgccacgag aagatgttcg acgagtggga gagctccgcg cacgcgcagg
    10261 ccgtgagctc cgagctctac aagaagagcg tcgccgcggc gaaagacgtc acgtgcgagc
    10321 gttgccatgc gccacttgcg agcgaggttc cgcgtgatgc gatcgtcacc gagggcgtca
    10381 cgtgcgacgt gtgccatacg ctgcgtgaac cgaagccgtc cgtcgacggt ggttcgtgga
    10441 agctcgcgat cgatgacatg gtgaagttcg gaccgcgctg cgatctgaag gatcactact
    10501 tccatcgcat gggctgctcg cccgagcaca agaccgccga aatctgcggc acctgccatt
    10561 ggtgggagcc caaaggcctg ccggtcttca cggagtacgc ggattggaag gcgggcccca
    10621 agcacgacac cccgtgccag gactgccaca tgccgaagag cagggcggcg ctcgcaacgg
    10681 gctccccggt acgagatgga gtgcctcatc acggcctgct cgggaaggcg aaggacctgc
    10741 gcaagacgtc gctgcatatg gacgcgaagc tcgcacccga cggcgcactg accgtgatgc
    10801 tcgagaacaa gcatgccgcg cactacgtgc cgtcgggtct gccggagcga cgcatcgtcg
    10861 tgcgtgcacg gctgctcgac gcgagcggcg cagaggcctg gcatgaccag cgcgagctcg
    10921 ggcggatgct cgtcgatgcg aatggcaagg aagcgccgtt ttgggcagcc acgcgcgtcg
    10981 gcagcgatac gcgaatcgcg cccgccggcc aatcgacgtt gacgttcgct gtccccgaac
    11041 ggaaggggcg cgtcgagatc gtcgtgctgt atcgtgggct ttcggaagcg atcgcgaagc
    11101 agctcggagt cagcgaggtc gaggagcaga agctgctcga gaagatcgtg aagaaatgaa
    11161 gctacgcacg accgtctttc tctgggtgct cttgctcgtc gttgtcgtgc tcggcgcgac
    11221 gatcggcacg atcgtgttcg tgttcaacaa ctcgacgcgc gaacgcgttg ccgacgagct
    11281 ggtgcgaagc cgcgacgtca cgctcgattt gcacgcgact cgcgattcgc tgcatcgcca
    11341 ggagtgccgc gtcgtcgccg acgagccgcg cttgcgcgcc gtggtagcca ccgaagacgt
    11401 cgcgcgcgag acgatcgtcg atgcagttcg tacgctcgca gacacgctgc acgcgggtgt
    11461 gttcgtgatc gtcgacgccg agggaaatct catcgccgac aatgcggtgc ccgatgcggt
    11521 cggcttctcg ctgatgaacc ggcccgaggt cgccgcggct ctcgagaaag gcgagggcac
    11581 cggcatctgg ctcgccgacg atcgcgtgta tcaggtgcaa gcttgccggc tcgagttcgg
    11641 tgcgcgcgtc gtcggtgcgc tcgtcgtcgg gcatgcgatc gataacgagc ttgcaaatgt
    11701 cgtcgcgcgg caaaccggcg ggtggctcgt cgtggtcgag gacaagctgc cgcgcaccga
    11761 gccgccgatc ggcacaaagc ccggcgaagt aggcgccgtc gtgcaggctg tgcgtgccgg
    11821 cgacaaagaa gtctcgctcg gcgacacgca ctacttcgcg cagctcgttc cggtccctgg
    11881 ctacaagggc gagcacaagg tgcagtacct cctgctgcgc tcgatcgacg aagcactcgc
    11941 gcccggcaag aagatcgttc gcatcctgat gggcctgctc ggcatcgccg tcctcgcgac
    12001 gctcgtgctc gcgctcggac tcgcccgccg gctgtcgcgg ccgatcgatt cgctcgtgac
    12061 gcgcacgctc gcaatcgcgc aaggcgatct gagggctcgt ccggtcagcg gaccgaccga
    12121 ggtgaaggca cttggctcgg cgatggatag gatggtcaaa gagatcgacg aatcgcgccg
    12181 atcgctcgcc gacaaagagc ggcttgctcg cgagatggaa atcgcggcgc gcattcagac
    12241 atcgatcttg ccgcgcaaca tcgctgtcga tggtctcgag atcacggcgc gcatgatgac
    12301 ggcgactgaa gtcggtggtg actactatga cgtgctcacc gtcgatggag gttgctggat
    12361 cgcggtcggc gatgcatcgg gccacggtct cacggcaggt ctcgtgatga tgatggtgca
    12421 gaccggcgtc gcgacgctcg tgcgtgcgaa accagacgcg aatccgaagg acgtgttgaa
    12481 gacgctgaac cgcgtcctgt tcgagaacgt gcacgaccgc ctggaggccg agcgccacat
    12541 gacgctgtgc ctgttgcgct atcgcggcgg cggcaagctg accgtcgcgg gtgcgcatat
    12601 ggatgcagtc gtctggcgcg ctgcgtctca gacggtcgag atgctgacga ccaagggcac
    12661 gttcctcgcc atcaccgacg atatcgacca cgtgaacgtc gagcaggaat ggtcacttgc
    12721 gcacggcgac ctgctcgtac tgcttaccga tggtgttacc gaagcggaga acgcggcggg
    12781 cacgccgttc ggctacgagg gcgtcgtaga agtcgtcgag ccgcgtgcga cgaagccagt
    12841 ggtcgcaatt cagaacgcac tgttcgacgc cgtgaccaag cattccccga cgctggctga
    12901 tgactgtaca attctagttc tgcgttacgt cggtccgggg gagacagacg gctgatggct
    12961 gagctccata tcaacgtcgg gatggttgcg ctatcccgcg gcttcatcac gctcgaggcg
    13021 ttcgccaagg gcatcgagaa gctcgcgcag tcggagcgtc aatcgatcca cgagctatgg
    13081 ggcaaccaca tgagcgacga ccagctcgcg atggtcctcg acgtgatcgg caaagtcgac
    13141 agcaaggcac ccgacacgat gatcgtgccg acgaacgtca aggcgatcgt gccgccgaca
    13201 ccgggtgcgc tctcgacgaa ggtcggccgc aagcagtcac cgacgggacc cggcactgcg
    13261 ccgactgccg cgtcgatcgt cgagctgccc ggggagagca aggcgcctcc ggtgccccgc
    13321 acgattacgc cgaacatgac gattccgatg ccgatggccg acctcctcgg tgctcggtac
    13381 aagaagctcg gcgtcctcgg caaaggcggt ctcggcgaag tcctgcagtg tgacgacccg
    13441 gtgctgcagc gaacggtcgc gctcaaagcc ggtcacgtga agggcgacgt cgacgcgtac
    13501 tcgacgaagg tcatcctagc gcgcgaggca cgcatcattg ccggcctcga gcatccgaac
    13561 atcatcccga tctacgacgc cggtgacgat ccgctgcgcg gtccctacta cgtcatgcgt
    13621 caggtcacgg acacctcgct cgaggcgatc ctgcaccagc gcaagaaggg cgagggcaac
    13681 ccgcacgact acacgctcaa tcgcctcctg cgctacttcc tacaggtctg caacgcggtc
    13741 gactacgcgc atcaccgcgg cgtgatccac tgcgacatca agcccgcgaa cattctcctc
    13801 ggtgattacg gcgaggttct cctcgtcgac tggggcctgg cgcagtcgcg caatcatccg
    13861 ctcggcgttc gcggcggcac gctcggctac atggcgccgg agcagatgga tccatcgatc
    13921 gaacggctcg attggcgcac cgacgtattc gcgctcggcg cgatcatcta cgagatcctg
    13981 tgcggcgtgc ccgcgttcga agaagcgcgg agctcggacc tcaccgcgat cggcaaggat
    14041 ccgaagcgcg tgtacaagct gccgctcgca ccgtcgcagc gagacagccg gctcgagatt
    14101 cccaaagagg tcgaagacgt ctgcatgcag gcgatcgccg tcgagcgcga gaagcggatc
    14161 gagaccgttc gcgagctcgc caacgcgatc gaagactgga tcgaaggtac gaagcagaag
    14221 gaacgccagc gcatcgaggc cgacaagagt gccgacgccg gcgacgagct tgccgagcgc
    14281 tatcacgagt tcgtcgagtc gcggcccgag aagatgacgg tgttccgcgc gctccgtgcc
    14341 gacgtcgcgc cgtgggcgga tctcgacgag aagcaagacc tgtgggacgc cgaggacatc
    14401 gtcaaggtga ccgacgcgct gcaagtgcgc acgttccatt cggccgtgtc ggcgtacgaa
    14461 cgcgcgctcg atcaggtgcc gaatcacacg cgcgcccgtc gcggtctcgc acgtctgtat
    14521 tggtcagagc tccagcgcgc gcggcaccga ggcgagacgc tcgatcaagt tgcgtacgag
    14581 cagttgctcc gcgaggtcga cgacggcacg ttcatgcgcg agctgcagcg cgacggtctg
    14641 ctcgagctga cgttcggcgc gcatgcaggc aaggtcacgc tcgcgccgct cgtcgagaaa
    14701 cagcgccggc tcgtcgaggg cgtagtcgaa gtgatcgaag agaggccgat ggcgcatcgc
    14761 tcgctcgcgg cgggtcgcta cgtcgtggcg acgtcgatcg gtgacggccc gcatcgcgtg
    14821 atgtggccgg tgaacatcga gccgggtgca ctgtgcaaaa tctcggtcga tccaccgcag
    14881 ctgtgtcgtg cgagcgagga tgaagtcctg gtgcccgaag gcatcgcgcg tctcggcggt
    14941 gatccgttct cgtccgatgc cgacgagctc gtgaccgtcg acgtgccgag ctttgtcatc
    15001 cagcgcttcc ccgtcacgtt cgcgcgctgg tacgagttca tcgacgatct gaagcgaacg
    15061 ggcataccgt tcgaggctca cgtgccgcgc acgccgatcg gtttgccgcc ggagccgcag
    15121 cgcctgcccg tgttcggtgt tagcgcggag tcggccgagg cg
//
LOCUS       DATLBZ010000343        20191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1144138, whole genome shotgun sequence.
ACCESSION   DATLBZ010000343 DATLBZ010000000
VERSION     DATLBZ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20191
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144138"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VMZ53_17685"
     CDS             <1..916
                     /locus_tag="VMZ53_17685"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HUS30347.1"
                     /translation="IPLGVIAMIYEARPNVTTDAAALCLKAGNACILRGGREALESNR
                     ALALAVQAGLAAAKLPVDAVQLVPLTDRDAIAELVKLDDLIDLCIPRGGEGLIRYVAE
                     HARVPVIKHYKGVCHVFVHAAANVDMALRIAENAKVQRPGVCNALETLLVDRAIARTF
                     IPALVQRLTPQVELRGDATVCELGGATVKRASEDDWSAEYLDLILAVRVVDGIADAIQ
                     HIEHYGSSHTESIVTEDRPIAEQFLREVDSSTVLVNASTRFADGGELGLGAEIGISTT
                     RLHAYGPMGAEGLTTTKFVVRGSGQIRT"
     gene            936..1439
                     /gene="rsfS"
                     /locus_tag="VMZ53_17690"
     CDS             936..1439
                     /gene="rsfS"
                     /locus_tag="VMZ53_17690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HUS30348.1"
                     /translation="MTKVKKSAPKAVRTEARGRSTAATHATTSTGQPTSTEGLDGALR
                     ALSLALDKKALEPIMLDVRQLCSFCNYQLVISGRSERQVDAIADGIAAGLKSEGLRAI
                     STEGARSGQWALLDFGDFVVHVFLHSVREHYDLEGLWNDAPRIPLDVPADARIPVDEH
                     YETPAVP"
     gene            1436..1864
                     /locus_tag="VMZ53_17695"
     CDS             1436..1864
                     /locus_tag="VMZ53_17695"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HUS30349.1"
                     /translation="MKIFLGAIGRLKEAYFTAAEEEYRKRLRPYCTLTVHEAKDEAAL
                     LAALPSGVQLYAFDERGEALTSQQFAELLAHEAQHGGGNPVAFLIGGADGHSPEVRKR
                     AKKLIAFGRLTIAHRLVRILVLEQLYRAFKIIKGEPYHRD"
     gene            1879..2859
                     /locus_tag="VMZ53_17700"
     CDS             1879..2859
                     /locus_tag="VMZ53_17700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUS30350.1"
                     /translation="MVPLSVLDLAHVVHGSTVAHALANTVDLAQHCERFGYHRFWLAE
                     HHNMAGIASAATSLVIGHVAHHTKTIRVGAGGIMLPNHSPLVIAEQFGTLETLFPGRI
                     DLGLGRAPGTDQRTLHFLRRDPSAADHFPQDVLDLRDLLGPHQPDQTVRAIPGVGTNV
                     PLWILGSSLFGAQLAAALGLPFAFASHFAPAALLKALQVYRELFRASDQLAKPHVMVA
                     CNVIAADTDAEARRLFTSIQQVFTNMMRGTRGKLSPPIDDIDAYWSPPEKAQAAAMLE
                     RSYVGSPSTVRDGLEMLIGETEADELIVASAIYDHAARVRSYELLASAAK"
     gene            complement(2893..3354)
                     /locus_tag="VMZ53_17705"
     CDS             complement(2893..3354)
                     /locus_tag="VMZ53_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HUS30351.1"
                     /translation="MRTWSSRLGSIEQGDTKHTEIKDQGITIPAALEQVCFATIGTAI
                     EVHRELGPGFLESVYEEAMAIELATRSIRFERQVAVPVMYKGHVVANHRLDLLIDSCV
                     IVELKAVTALEPVHAAQVISYLKATGVQVGLLVNFNVPLLKNGIRRFVWDL"
     gene            complement(3407..4174)
                     /locus_tag="VMZ53_17710"
     CDS             complement(3407..4174)
                     /locus_tag="VMZ53_17710"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HUS30352.1"
                     /translation="MTKYLALALALLAACGGAPKPAGPSPVPVPLGSNATQDSGPSTT
                     EPPPDAADRKHAFARLPTELSWNGNNAALADDIRFQRVQQGAAFDITEGPVVVVQGKV
                     TTSQNQEVAYWAKKPHRIQCAKGADCILFVHANAPAESRELFAQDIKWTQLDPSRPDP
                     LVAPLWGDGTKEPNGFLMKMKAGGGPFWHIHRRDYHGVVLVGNVVSYESGTDAREMPP
                     GSYWWQPNGNKHTTDCKGPSDCVIYLDFTGPFDVKAL"
     gene            complement(4177..4653)
                     /gene="smpB"
                     /locus_tag="VMZ53_17715"
     CDS             complement(4177..4653)
                     /gene="smpB"
                     /locus_tag="VMZ53_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HUS30353.1"
                     /translation="MKAKEGDIVKVIATNRKATHDFHIHDRMEAGIQLVGSEVKSLRD
                     ARVVIADGFVEFRKGQAYLHKVSINEYSQANRWGHDLERVRKLLLHKHEIDKLATKVQ
                     QKGFTLIPLSLYFKGHRVKVELALVTHKKHHDKRATKKEADDKREMDRAMKNSRKD"
     gene            complement(4658..5083)
                     /locus_tag="VMZ53_17720"
     CDS             complement(4658..5083)
                     /locus_tag="VMZ53_17720"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS30354.1"
                     /translation="MRYLHTMVRVRDLPAALRFFCEGLGLREVRRRDSEQGRFTLVFL
                     GEKDSGDSPQIELTHNWDQTEPYTGGRNFGHVAYEVDDIYAACQRLIDCGYTISRPPR
                     DGRMAFVRSPDLISVELLQRGEALAPAEPWTSMKNVGEW"
     gene            5119..5526
                     /locus_tag="VMZ53_17725"
     CDS             5119..5526
                     /locus_tag="VMZ53_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30355.1"
                     /translation="MSAPNVLVNFRLDKDRALRLKWLADDGAPGDGYKAQITAIKLMD
                     GARLAMDKSAILEQTMPDPDGGVGGYLMTFNGMVGFAADHPDRKLVEALVDQEIEYEL
                     ELVHDESGRVAVENTDFRIVERPRGLAKKLPSK"
     gene            5523..6410
                     /locus_tag="VMZ53_17730"
     CDS             5523..6410
                     /locus_tag="VMZ53_17730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUS30356.1"
                     /translation="MRDETPSRTAAFVAAVRQLGQLLPPDARLVEDPYGAAFTSATVG
                     RALERNADQLRLLAAMPGMKNWILYMQVRTRVIDDAVREFVEAGGRQVVVLGAGYDTR
                     ALRLPELVDADVYEIDHPATQGHKRRTLERIGAQSPAHYVTWDFEARPMEDLPDELAG
                     AGLDLGAPVFTIWEGVTMYLTEPAIDASLRAIRQWSAAGSRLAMTYVIRSRLERPSLI
                     SRAMQMVVSSVGEPWKWGWDPDELPEYLAARDFTLLRDVPMRDAARELLPPQMAIHVP
                     HRDRRIAVAAGAESIALAK"
     gene            6492..7091
                     /locus_tag="VMZ53_17735"
     CDS             6492..7091
                     /locus_tag="VMZ53_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30357.1"
                     /translation="MQKLLLAAIASLGFTGCYTSGDVGAGYSTSGGYYAGGGGYYAEP
                     QLYYYAPGISVVAYSDDPVFYSDGLYWRYYGNTWYSSNYYGSGWSVAYNVPYGVRSID
                     RPHTYAHFTPGSGWTRVHANGGAYAGGNYNNGGPVVRDHRTGYNPPARTYQAPAQSTP
                     VVRDHRSAPTYAPTPAPTTYAPRPAPAPSGPVVRDHRRH"
     gene            complement(7146..8276)
                     /locus_tag="VMZ53_17740"
     CDS             complement(7146..8276)
                     /locus_tag="VMZ53_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30358.1"
                     /translation="MSTYVHLARYGKKPGSAQVSAVEVLSEDEVLYGGHRWSIRTGLR
                     GERQPGFGHSLDGRFLEWKDSGAGYVLEVVSKSKRVALGMVGSANQFFIVGDRVLVND
                     DMRQPWLVFDANTGEKIGRLVAMMPDATLGAPMYTPGLFSPNDRRTLWFGEKARGAQI
                     DLVKREVVRAVLPAAEHAFIGAVVARGDLVLALERPLKNVTSNVRDDDRLVLFDANGA
                     RIAERPGSTSGHSLLGDRFLAVDTIGKRFILLDDTLQIVEEVPMFDQGKVPFARLVTL
                     PSGREWIAIGGRGEWDHYGDPSLAPSTKPVEVANKPRTRPPPKALPPTEKLAPRTPPK
                     VTSKSAPAPKAAAKATPAPKAASKPAAKPAAKPAAKPAPKKR"
     gene            complement(8273..11866)
                     /locus_tag="VMZ53_17745"
     CDS             complement(8273..11866)
                     /locus_tag="VMZ53_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30359.1"
                     /translation="MRRIVSLFALILTTCSPPQPMKTTTATVGGGGGVVAPPAPVADA
                     NLDTLTEGAKVRGFTAVALYLDDADKPLGARFTHDATGFTFDYLRIETAPQGFIWVNS
                     FPTGDKGEPHTQEHLLLGKGDRGRKLGSFEAMALAESSAFTDQWRTAYHFHTVAGPSV
                     FWPVFEDQLDALLNPDYSDEEIRREVRNFGVDKADDGTLHLEEKGTVYNEMVRTYESP
                     EVNLWREAGHLVYGPKHPLVLDSGGYPPAIRDMTPQDIRAFHESAYHLANMGMIAAFP
                     TSMSLASVLDETSKILVKEAGRKGRVMSEADLPKPTPAAAGTVKVVDYPYATATNPGP
                     MMFAWPASRDLDDTERVLMGLFLDSFAGDESTTLYKQLIDSKTRKMDLGASDVWSYTK
                     TDQGQPVYLGLSGVKADKLEAKTVSDVRALIAAELERIAKLPDGDPELVAFDARVQSR
                     VIDLRRRLAKFLDSPPGFGIRGTGSGWNDHLHQLSKSKGFKKSLTLRPALAQVEQILA
                     GKGNPWRERLKTWGLLETPFGIAAKPSPELRKQLDDERKARADAELARLQKQYAAKDA
                     AATLAKYATDYDAETKRMEAAEGANQLPPLVDSPPMTLDDSLKYETDAVASVPRFTAT
                     FDAMVSSRVQLAFRIGAIADKDQVFLAALPSLLSDAGVIENGTPIPADEMKERQRKEI
                     LELSVYYVDNPKSGRIELVAAGAGNGAGETKAAIGWLHRVLLSPDWRPENLARLRDVI
                     DQRLTGIRQRMLGAEEGWVENPRDAWRVGTALYGHTSSFLTRAHDLHRIRWMLADPGE
                     AAATKEVVKFLGTLGGAKSLKRDQLAALTDALAKDTKPKDAKLASYVAAAGKLSAKAK
                     PLALMAGKDLAAMLGDLPDSSLAADWTYLCKQMAADLQVGAPAALARLESVRKAVVDG
                     GGARIVEVGSKANQQAIASDVQKLVGALPKQKQAAAPAATGNVFRDRLAARDPKAKTA
                     TFVGLFAPSTSSGVFVNLAPNGYFGDASDDSVMDYLASNLYTGHGAHSIFMKTWAAGL
                     AYSNGMRRDLEEGTVLYYAERCPLLPQTLRFVIDQLKKGSKPDANIARYAVAKAFEAR
                     VANGFEQRASAMASNLVDGLTPDVQKAFREKVIAASKDPQLADKLFGRMEKVYSKVLP
                     GYGSLDPKAIYFVIGPDKQLTAYQEYLRAAVGKDATLHRLYPRDFWIPATL"
     gene            complement(11928..13304)
                     /locus_tag="VMZ53_17750"
     CDS             complement(11928..13304)
                     /locus_tag="VMZ53_17750"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUS30360.1"
                     /translation="MTIRLSDLDRCLHGIVPGVIATCDRDAMPNVSYVSELHLIDDQH
                     VALSCQFFNKTRQNLDENPLATVEVYDPITFDAYRLRLKFERSETTGPLFEAMSTRIQ
                     AIAFHTGMAGVFKLRSADVFEVLSIEQREGYIEPPPQGERIVRMLPDGPLTELRGLQL
                     VSERCARATDLESLLGGALAALDEVFGFSHGMILVPDETGQRLIAIASHGYGDAAIGA
                     EVKIGEGVIGSVADGRQIVRIGCVSSELRYGRAIRAQYEQTGKAGLGEEIPLPHLVDA
                     ESQMSIPLMVGDRLVAVLALECRNSMTFAQWHEAFLQVLANQIAGGIDRMLEREDDGA
                     PLPVTRASARPAASARKRTFHYYRNDDCVFVDGEYLIRNVPAKILWKILRAYETEKRT
                     EFSNRELRLDPSLGLPAFKDNLESRLILLRKRLEQKCTDIQLVPTRRGRFSLAVDCAI
                     ELVERETA"
     gene            complement(13301..13798)
                     /locus_tag="VMZ53_17755"
     CDS             complement(13301..13798)
                     /locus_tag="VMZ53_17755"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUS30361.1"
                     /translation="MSASYPPLPPELVAFAHGGVSILVGTCSRDGVPDCVRGMGVRIW
                     PDACHLTLIMPAKTGAQSIANLATNPRLAVTLSHIPSHKTVQVKGNVLAIRDGNADDR
                     ALAEKYRVLLAEDLAFVGQPAANTLRLGIWPCHAIDLAIELVFAQTPGPSAGVKLPMS
                     AEKSL"
     gene            complement(13810..14373)
                     /locus_tag="VMZ53_17760"
     CDS             complement(13810..14373)
                     /locus_tag="VMZ53_17760"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUS30362.1"
                     /translation="MRVDLFSMIHKGVRSLLFEVALEAARVDLTSAAAIDNLASRIDT
                     VLQFLDEHARIEETYVMPALRALDAALAEELGAEHRALEVVQIEVERATAALEQADLA
                     ARGEAGAKLIRVVNHLVSVQLVHMNREETEIMATLWAGLKDVDLGALRMRMAESLPAE
                     RRLAWRGVLEASISPVERRLVLGAPLG"
     gene            complement(14373..14978)
                     /locus_tag="VMZ53_17765"
     CDS             complement(14373..14978)
                     /locus_tag="VMZ53_17765"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS30363.1"
                     /translation="MKTMILLSALVGAQAACKSDSAPAKGADPALAKRAEIERGQHLV
                     MTSGCHDCHTPLKMGPNGPEPDFSRELSGHPHDFIVPPPPASSGPWVASILATNTAWA
                     GPWGVSFTRNLTPDKETGLGSWTKQMFIDTIRNGRHMGVGRPLLPPMPFPMYKHMTDD
                     ELGAVFAYLQSLPPIKNAVPEPLPPQQASAPAAAADKTARL"
     gene            complement(15001..16005)
                     /locus_tag="VMZ53_17770"
     CDS             complement(15001..16005)
                     /locus_tag="VMZ53_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30364.1"
                     /translation="MKTTASRMLAATALVGAGVATAVAGSLIRQTERALPEPGLVHAP
                     QTLRETGLYAGDAIDASNEAFAPQYPLWTDGAEKQRWIYLPPGTQIDASDPDAWQFPV
                     GTKLWKEFSFGGVPVETRFMVRTRDGWEFATYVWAGGEAVRTPDGASTIELAAGVRHR
                     VPSEGECRACHGNGSSPVLGFSALQLSIDRDPNAPHSQAAQLDLASLVMTGRIRGVTN
                     IAPRIPGAPVERAALGYLHGNCGHCHRREGAVESVGMVLSQSLVEPGRVERTIVGQPS
                     RFMAPALRVQPGASKLSVVIARMRSRNPLTQMPPLGTQLVDDEATHLIATWIDQLATN
                     "
     gene            16127..17821
                     /locus_tag="VMZ53_17775"
     CDS             16127..17821
                     /locus_tag="VMZ53_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30365.1"
                     /translation="MRWFASLLLAGCMHSVAAPTIPPHPGMGSVHFAIDTRAPAAQQH
                     FDAGLAALYGFNYDEAHYEFLVASHADPSCAMCAWGFAYASGPNINAILKQYQGTYES
                     AQQAATLARTPLEKAITKALVARLSPHMMVSTDERSKLDDAYLAAMREAAKLAPSDPD
                     VQTFLAEALWMATPIGVPGYTKDGIATHPNVTEAVSVLEHVLEAHPDHIGAIHFYIHA
                     VDGSKAMEKAVPFAQKLATLAPESGHLVHMPSHLMLKLGRYAEAIDENRAAIASDKRY
                     LARNPKGGEYEMFSMHPRQYLWYVLLFTGGSREATTLASELRDEMQKMAMMDPHAADQ
                     AAVFAALVAARFGNWDTALSLPPPSGSMSTIATSFARGLALVAKGKLDEAAVEVEKIR
                     KAPDADTPPSPPPAPAAHGDHADTPAEAPPPMGGPPELFVKKQREYTRALSAAFAAQL
                     SGAVTAARGKQEEAIAELRGAVDLEDAVPDMGELSKPPVTARQRLGALLLTAGRPADA
                     ERVYLADLKVFPENGWSLFGLWKALDAQHKPDAAAARARFEAAWKHADIQLTSSVF"
     gene            17846..19375
                     /locus_tag="VMZ53_17780"
     CDS             17846..19375
                     /locus_tag="VMZ53_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30366.1"
                     /translation="MKRGIAGVAAAVMLCATSCVHRIERKSGTTIHTINDKAPFLKVH
                     MPDGKLYVLADWRVDETTKHITGTGDLFDADRELEKTGSFSIAMDDVAMYETNTIESS
                     PGVAAMAVISGLSVAVTAFCIANPKSCFGSCPTFYAPSDDNGRPVLQAEGFSDSIAPS
                     LERNDIDALWQTTGHAGRTTITMTNEAYETHVVKQVDLLAVPRPAHGRVLATDDHLYI
                     ASTLTAPTACIASEGSCLDALSTVDGRERSSTTDGEDLATRETIQLAFPRGDGATGQR
                     AIVIAARQSLVTTFLLYQGLAYLGADATRWLATLGSMNADARVGGRALHRLVGGIEVQ
                     VRDGDGWRTVDEVYETGPIATDVHLVLLPVAAAGDEIRLVLQRGGWRIDSVALATITG
                     EAQPIRIAPSKIRGTLGAEYAGARKPASRFPIITLPGDRYELEYTLPATDAAYELFLD
                     SRGYYLEWMRDEWLADQNTLSAIKLFADPEAMLRELAPAYKRVEPHMEEIFWRSRYAR
                     P"
     gene            19362..20081
                     /locus_tag="VMZ53_17785"
     CDS             19362..20081
                     /locus_tag="VMZ53_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30367.1"
                     /translation="MRALSVLLAFALTTACIYNPDPREPTLADTQRKGFGAWIVVTAR
                     DGSETQGELLAIDNGSLFIKTRVRKPVVIPLHTVQGADVYTYESDWGFGVWGLLGTLS
                     TASHGLILIFSAPIWMLSASIAAGVESAHIRLQIPDDDIDEIRKWARYPQGMPKQSPT
                     ISRKEDAWAFTQQAQVAARMGNCEAVTKLEPRVLELDVGLHDMTFMRDEAIRRCLGLP
                     SLIAPPSMVPPSTPPDAGVTP"
BASE COUNT         3392 a         6847 c         6467 g         3485 t
ORIGIN      
        1 catccctctc ggtgtgatcg cgatgatcta cgaagcgcgc ccgaacgtga cgaccgacgc
       61 cgcagcgctc tgcctcaaag ccggcaacgc atgcatcctg cgcggtggtc gcgaagcgct
      121 cgagagcaat cgcgcactcg cccttgcagt gcaagccggg ctcgctgcgg cgaagctccc
      181 cgtcgacgcc gtccagctcg tcccgctcac cgatcgcgat gcgatcgccg agctcgtcaa
      241 gctcgacgac ctgatcgacc tctgcatccc gcgcggcggt gaaggcctca tccgctacgt
      301 cgccgagcac gcgcgcgtgc cggtcatcaa gcactacaaa ggcgtctgtc acgtattcgt
      361 gcacgctgca gcgaacgtcg acatggctct tcgcatcgcg gaaaacgcga aggtgcagcg
      421 gcctggcgta tgcaacgctc tagagacgtt gctcgtcgat cgcgcaatag ctcggacatt
      481 cattccagcg ctcgtacagc gcctcactcc gcaagtcgag ttgcgcggtg acgccacggt
      541 ctgcgagctc ggcggcgcca cggtgaaacg cgcctccgaa gacgactggt ctgcggaata
      601 tctggacctc atcctcgcgg tccgcgtcgt cgatggaatc gcagacgcca ttcagcacat
      661 cgagcattac ggcagctcgc acactgaatc gatcgtgacg gaagaccgtc ccatcgcaga
      721 gcagttcttg cgagaagtcg attcgtccac cgtgctcgtc aacgcgtcga cgcgattcgc
      781 cgacggcgga gagctcggcc ttggcgccga aatcggaatc tcgacgacgc gcctccacgc
      841 ctacggcccg atgggagccg aaggcctgac caccaccaag tttgtcgtcc gcggcagcgg
      901 acagattcgt acgtgaaggg agaacgaacc gaggcatgac aaaggtaaag aagagcgccc
      961 ctaaagctgt ccgcaccgaa gcacgcggcc gtagcaccgc cgcgacccac gcaacgactt
     1021 cgacgggcca gccaactagt accgaagggc tcgacggcgc gttgcgcgcc ctctcgctcg
     1081 ctctcgacaa gaaggcgctc gagccgatca tgctcgacgt tcggcaactc tgttcgttct
     1141 gcaactacca gctggtgatc agcggtcgct ccgaacgcca agtcgatgcg atcgccgacg
     1201 gcatcgccgc gggcctcaaa tccgaaggcc tccgcgcgat cagcaccgaa ggcgcgcgca
     1261 gcggccagtg ggccctgctc gacttcggcg acttcgtcgt ccacgtgttc cttcactcgg
     1321 tgcgcgagca ctatgacctc gaaggtctgt ggaacgacgc gccgcggatc ccgctcgacg
     1381 ttccggccga cgcacgcatc cccgtcgacg agcattacga gacgcccgca gttccgtgaa
     1441 gatcttcctc ggcgcgatcg gccgcctcaa agaggcgtac ttcaccgccg ccgaggagga
     1501 atatcgcaag cgccttcgtc cctactgcac gctgacggtg cacgaagcga aagacgaagc
     1561 cgcgctgctc gctgctctgc cgagcggtgt gcagctctat gcattcgacg agcgtggcga
     1621 ggctctcacc agccagcagt tcgcggagct actcgcgcat gaagcgcagc acggcggcgg
     1681 caaccccgtc gcgttcttga tcggcggcgc cgacggccac tcgccggaag tccgcaagcg
     1741 cgcgaagaag ctgatcgcgt tcggccgcct gacgatcgcg catcggctcg tcaggatctt
     1801 ggtcctcgag cagctctacc gcgcattcaa gatcatcaag ggcgagccct accaccgcga
     1861 ctagggtaag cagtgggcgt ggttccgctg tccgtcctcg atctcgcgca cgtcgtccac
     1921 ggctcgacgg tcgcgcacgc gctcgccaac acggtcgacc tagcccagca ttgcgaacgc
     1981 ttcggctatc accgcttctg gctcgccgag caccacaaca tggcgggcat cgcgagcgca
     2041 gcgacgagcc tcgtcatcgg tcacgtcgcc caccacacga agacgatccg cgtcggcgca
     2101 ggcggcatca tgctgccgaa ccactcgcct ctcgtgatcg ccgaacaatt cggcacgctc
     2161 gagacgctgt tccccggccg catcgacctc ggcctcggcc gcgcgcccgg caccgaccaa
     2221 cgcacgctcc acttccttcg ccgcgatcca tccgccgccg atcactttcc gcaagacgtc
     2281 ctcgacctgc gcgacctcct cggcccgcac caacccgacc aaaccgtccg cgcgattcca
     2341 ggcgtcggca cgaacgtccc gctctggatc ctcggctcga gcctgttcgg cgcgcagctc
     2401 gcggccgcgc tcggcctgcc cttcgcgttc gcgtcgcact tcgcgcccgc cgcgctgctc
     2461 aaagcgctcc aggtgtaccg cgagctgttc cgcgcgtcag accagctcgc gaagccgcac
     2521 gtcatggtcg cgtgcaacgt gatcgcggcc gacaccgacg ccgaagcgcg ccgcctcttc
     2581 acatcgatcc agcaggtgtt cacgaacatg atgcgcggca cgcgcggcaa gctgtcgcct
     2641 ccaatcgacg acatcgacgc gtattggtcg ccgcccgaaa aggcgcaggc cgccgcgatg
     2701 ctcgagcgct cctacgtcgg ctcgccgtcg acggttcgcg atggcctcga gatgctcatc
     2761 ggcgagacag aagccgacga gctgatcgtc gcctccgcga tctacgacca cgcggcgcgc
     2821 gttcgctcct acgagctgct cgcctccgcg gcgaaatgaa gaattaaaca gggagacacg
     2881 gagacacgaa ggtcagagat cccagacgaa gcgccggatt ccatttttga gcagcggcac
     2941 gttgaagttg accagcagcc caacctgcac acctgtcgcc ttgaggtacg agatgacttg
     3001 cgcggcgtgt accggctcca gcgcggtcac cgctttcagc tcgacgatca cgcagctatc
     3061 gatgagtaga tcgaggcggt gattcgccac gacgtgtccc ttgtacatca caggtacggc
     3121 cacttggcgc tcaaatcgta tggagcgggt cgcgagctcg atggccatcg cttcctcgta
     3181 cacggactcg agaaagcctg gtcccaattc acggtggacc tcgatcgcag ttccgatggt
     3241 cgcgaagcag acctgctcca gcgccgcggg aatcgtgatc ccctggtctt tgatctccgt
     3301 gtgcttcgtg tctccctgtt caatcgatcc gagacgagag ctccaagtgc gcatgggcag
     3361 ggatatcggc acgtcccgcg acgggttgcg tgcgaatggt cgtccgctac agcgccttca
     3421 cgtcgaacgg gcccgtgaag tcgaggtaga tcacgcagtc gctcggacct ttgcagtcgg
     3481 tcgtgtgctt gttgccgttt ggctgccacc agtacgagcc cggcggcatc tcgcgcgcat
     3541 cggtgccgga ctcgtagctg acgacgtttc cgacgaggac gacgccgtga tagtcgcggc
     3601 ggtggatgtg ccagaacggg ccaccgcctg ctttcatctt catcaggaag ccgttcggct
     3661 ccttcgtgcc gtcgccccag agcggcgcga cgagcggatc ggggcgcgat ggatcgagct
     3721 gcgtccactt gatgtcttgg gcgaacagct cgcgcgactc ggccggcgcg ttggcgtgga
     3781 caaacaggat gcagtcggcg cccttcgcgc actggatgcg gtgcggcttc ttcgcccagt
     3841 acgcgacttc ttgattctgc gacgtcgtga ccttgccttg cacgacgacg accgggccct
     3901 cggtgatgtc gaaggccgcg ccctgctgga cgcgctggaa gcgaatgtcg tcggcgagcg
     3961 cggcattgtt gccgttccac gagagctccg tggggaggcg cgcgaacgcg tgcttgcggt
     4021 cggcagcgtc gggcggcggc tcggtcgtcg atgggccgga gtcctgcgtg gcgttgctgc
     4081 cgagcgggac tggcaccggc gacggtccgg ctggtttcgg cgcgccaccg catgcagcga
     4141 gcagcgcgag ggcgagtgcg aggtacttgg tcatcgtcag tccttgcggc tgttcttcat
     4201 cgcgcgatcc atctcgcgct tgtcgtccgc ttcttttttc gtcgcgcgct tgtcgtggtg
     4261 cttcttgtgc gtgacgagcg cgagctcgac tttgacgcgg tggcctttga agtagagcga
     4321 gagcgggatc agcgtgaagc ccttttgctg gaccttcgtc gcgagcttgt cgatctcgtg
     4381 cttgtggagc agcagcttgc ggacgcgctc gaggtcgtgg ccccagcggt tcgcctgcga
     4441 gtactcgttg atcgagacct tgtggaggta cgcctggccc ttgcggaact ctacgaagcc
     4501 gtcggcgatc acgacgcgcg cgtcacgcag cgacttcacc tcggagccga cgagctggat
     4561 gcccgcctcc atgcggtcgt gaatgtggaa gtcgtgcgtc gccttgcggt tcgtcgcgat
     4621 gactttgacg atgtcgcctt ctttggcttt cactgcgtca ccactcgccg acgttcttca
     4681 tcgaggtcca cggctcggcg ggcgcgaggg cttcgccgcg ttggagcagc tcgacgctga
     4741 tgaggtccgg ggagcggacg aacgccatgc ggccgtcgcg tggcgggcgc gagatcgtgt
     4801 agccgcaatc gatcaggcgt tggcaggccg catagatgtc gtcgacctcg tacgcgacgt
     4861 ggccgaagtt gcggccgccc gtgtagggct cggtttggtc ccagttgtgc gtcagctcga
     4921 tctgcggcga gtcgccggag tccttctcgc cgaggaacac gagcgtgaag cggccctgct
     4981 cgctgtcgcg gcgtcgcacc tcgcgcaggc ccagtccctc gcagaagaag cggagggcgg
     5041 ccggcaggtc gcggacgcga accatcgtgt gcaggtagcg catgacacgt ccgtagcatt
     5101 cttgggtatg tttgcgccat gagcgcgccc aatgtcctcg tgaacttccg gctcgataag
     5161 gatcgagcgc tacggctcaa gtggctggcg gacgacggcg cgcccggcga cggctacaag
     5221 gcgcagatta ctgccatcaa gctgatggac ggcgcgcggc tcgcgatgga caagagcgcc
     5281 atcctcgagc agacgatgcc tgaccccgac ggcggtgtcg gcgggtatct catgacgttc
     5341 aacggcatgg tgggcttcgc ggccgatcat cctgatcgca agctcgtcga ggcgctcgtc
     5401 gatcaagaga tcgaatacga gctcgagctg gtgcacgacg agagtggccg cgtcgccgtc
     5461 gagaacaccg acttccgtat cgtcgaacgc ccgcgcggtc tggccaagaa gctgccctcc
     5521 aaatgagaga cgagacgccg agtcgcacgg cggcatttgt cgcggctgtc cgccaactcg
     5581 gccagctgct cccgcccgat gcgcggctcg tcgaggatcc ctacggtgcc gcgttcacgt
     5641 cggcgacggt cggtcgcgcg ctcgagcgca acgccgatca gcttcggttg ctcgctgcga
     5701 tgccgggcat gaagaactgg atcctctaca tgcaggtccg cacgcgcgtt atcgacgatg
     5761 ccgtgcgcga gttcgtcgag gcgggtggcc gccaggtcgt cgtgctcggc gccggttacg
     5821 atactcgcgc gctgcgcctc cccgagctcg tcgacgcgga cgtctacgaa atcgatcatc
     5881 cggcaacgca aggccacaag cgccgcacgc tcgagcgcat cggtgcccag tcgcctgcgc
     5941 actacgtgac gtgggacttc gaagcgcggc cgatggaaga tctgcccgac gagctcgcag
     6001 gcgccggtct agacctaggc gcgcccgtct tcacgatctg ggaaggcgtc acgatgtacc
     6061 tgacggaacc ggcgatcgat gcctcgctgc gcgccattcg ccagtggagc gccgcgggct
     6121 cgcgcctcgc catgacgtac gtgatccggt ctcgcctcga gcgcccgtct ctcatctcgc
     6181 gcgccatgca gatggtcgtg tcgtccgtcg gtgagccatg gaagtggggc tgggatccgg
     6241 acgagctgcc cgaatacctc gcagcgcgcg acttcacact tcttcgcgat gtgcccatgc
     6301 gcgacgctgc gcgcgagctg ctgccgccgc aaatggcgat tcacgtgccg catcgagacc
     6361 gccgaatcgc cgtcgcggcg ggcgcggagt cgatcgcact ggcaaagtga cgctcgccac
     6421 gacgcgcatc caggcactta cgagtgtcaa gaggtgcatc gccgtcgggc cagcgctacc
     6481 attctcccat catgcagaaa ctgctcttgg ctgcgatcgc gtccctcggc ttcaccggct
     6541 gttacaccag cggtgacgtc ggtgccgggt actcgacgag tggcggctac tacgccggtg
     6601 gcggcggcta ctacgccgag ccgcagctct actactacgc gcctggtatc tcggtcgtcg
     6661 cgtactccga cgatcccgtc ttctacagcg atggcctgta ttggcgctac tacgggaaca
     6721 cttggtacag ctcgaactac tacggtagcg gctggagcgt cgcgtacaac gtgccgtacg
     6781 gcgtgcgctc catcgatcgc ccgcacacgt acgctcactt cacgccgggc agcggctgga
     6841 ctcgcgtcca cgccaacggc ggcgcgtacg caggcggcaa ctacaacaac ggcggccctg
     6901 tcgttcgcga tcaccgcacc ggctacaacc cgccggcgcg cacgtaccag gcgcccgcgc
     6961 agagcacgcc ggtcgttcgc gatcaccgca gcgcgccgac gtacgcaccg acgcccgccc
     7021 cgaccacgta cgcaccgcgc ccggccccgg cgccgagcgg ccccgtcgtt cgcgaccatc
     7081 gccgtcacta accgctcgcg gtcgtgactc gcagcgccgg cccgtgccgg cgttttcgtt
     7141 ttcggtcagc gcttcttcgg tgcgggcttc gcggctggct tggccgccgg cttcgctgcc
     7201 ggcttcgatg cggccttcgg cgcgggcgtc gccttcgcag cggccttcgg tgcaggcgcc
     7261 gacttcgagg tgaccttcgg cggcgtcctc ggagcgagct tttccgtcgg cggaagcgcc
     7321 ttgggcggcg ggcgcgtgcg cggcttgttc gcgacctcga cgggcttcgt cgacggagcg
     7381 agcgacggat cgccgtaatg atcccattcg ccgcgaccgc cgatcgcgat ccactcgcga
     7441 ccactcggca gcgttacgag gcgcgcgaac gggaccttgc cttggtcgaa catcggcact
     7501 tcttcgacga tctggagcgt gtcgtcgagg agaatgaagc gtttgccgat cgtatcgacg
     7561 gcgaggaagc gatcgccgag gagcgagtgc cccgacgtcg agccgggtcg ttccgcgatg
     7621 cgcgcgccat tggcgtcgaa cagcacgagc cgatcgtcgt cgcgcacgtt gctcgtgacg
     7681 ttcttcagcg ggcgctcgag tgcgagcacg aggtcccctc gcgccacgac cgcaccgatg
     7741 aacgcgtgct ccgccgcggg cagcaccgcg cgaacgacct ctcgcttcac gagatcgatc
     7801 tgcgcgccgc gcgctttctc gccgaaccag agcgttctgc gatcattcgg cgaaaacaga
     7861 cccggcgtgt acatcggcgc gccgagcgtc gcgtccggca tcatggcgac gagccggcca
     7921 atcttctcgc cggtgttcgc gtcgaacacg agccacggct ggcgcatgtc gtcgttgacg
     7981 agcacgcggt cgcccacgat gaagaattgg ttcgcggagc ccaccatgcc gagcgcgacg
     8041 cgcttcgact tgctcacgac ctcgagcacg tagccagcgc cactgtcctt ccattcgagg
     8101 aagcgaccgt cgagcgagtg cccgaatccg ggctgcctct ctccgcgcag accggtgcgt
     8161 atgctccaac ggtgacctcc gtagaggacc tcgtcttcgc tgaggacttc gactgccgag
     8221 acttgggcgg aacccggctt cttgccgtat cgcgctagat gaacgtaggt actcatagcg
     8281 ttgctggaat ccagaagtcg cgtgggtaga ggcgatggag ggtcgcgtcc ttgccgactg
     8341 cggcgcgaag atactcctga taggcggtca gctgcttgtc tggaccaata acgaagtaga
     8401 tcgccttcgg atcgagggat ccgtagcccg ggagcacctt gctgtacacc ttctccatcc
     8461 ggccgaaaag cttgtccgca agctggggat ctttcgacgc cgcgatcacc ttctcacgga
     8521 acgctttctg cacgtccggg gtgaggccat cgaccaggtt cgacgccatc gcgctcgccc
     8581 gctgctcgaa gccgttcgcg acgcgtgcct cgaatgcctt ggcgacggcg taccgcgcga
     8641 tgtttgcatc gggcttgctg cccttcttga gctggtcgat cacgaaccgc agcgtctgcg
     8701 gcaacagcgg gcagcgctcg gcgtagtaaa ggaccgttcc ttcttcgaga tcgcggcgca
     8761 tgccgttcga gtacgcgagg cccgcggccc acgtcttcat gaagatcgag tgcgcaccgt
     8821 ggccggtgta gaggttcgac gcgaggtagt ccatcaccga gtcgtcgctc gcgtcaccga
     8881 agtagccgtt cggcgcgagg ttgacgaaca cgccactgga cgtcgagggc gcgaacaagc
     8941 cgacgaacgt cgcggtcttc gctttcggat cgcgggcggc caggcgatcg cggaacacat
     9001 tgccggtggc tgcgggcgca gcagcctgct tctgcttcgg cagcgcaccg acgagcttct
     9061 gtacgtccga cgcgatcgcc tgctggttcg ccttgctgcc gacctcgacg atccgcgcgc
     9121 cgcctccgtc gacgaccgct ttgcgcaccg actcgagacg agcgagcgct gccggcgcgc
     9181 cgacctggag atccgccgcc atctgcttgc acagatacgt ccagtccgcg gcgagcgagc
     9241 tatcgggcag atcgccgagc atcgccgcga ggtccttgcc cgccatcagc gcgagcggct
     9301 ttgccttcgc ggacagcttg ccagcggccg ccacgtacga cgcgagcttc gcgtccttgg
     9361 gcttcgtgtc cttcgcgagc gcgtcggtca gtgccgcgag ctggtcgcgc ttcagcgact
     9421 tcgcgccgcc gagcgtgccg aggaacttca cgacttcctt cgtcgccgcc gcctcccccg
     9481 gatccgcgag catccagcgg atgcgatgaa ggtcatgcgc acgcgtcaag aacgacgacg
     9541 tgtgaccgta gagcgccgtg ccgactcgcc acgcatcgcg cgggttctcg acccaacctt
     9601 cttcggcgcc gagcatgcgc tggcggatgc cggtgagccg ctgatcgatc acgtcgcgca
     9661 ggcgagccag gttctccggg cgccagtccg gcgacagcag cacgcggtgg agccagccga
     9721 tcgcggcctt tgtctcgccc gcgccattgc cggcgcccgc ggcgacgagc tcgatgcgtc
     9781 cgctcttggg attgtcgacg tagtaaacgc tcagctcgag gatctctttg cgctggcgct
     9841 ccttcatctc gtcggcggga atcggcgtgc cgttctcgat cacgcctgcg tcggagagga
     9901 ggctcggcag cgcggcgagg aacacctggt ctttgtctgc aatcgcgccg atgcgaaatg
     9961 cgagctgcac gcgcgagctc accatcgcat cgaacgtcgc ggtgaagcgc ggcaccgagg
    10021 cgactgcatc ggtctcgtac ttcagcgaat cgtcgagcgt catcggcggc gaatcgacga
    10081 gcggcggcag ctggtttgct ccctccgcag cctccatgcg cttggtctcg gcgtcgtaat
    10141 cggtggcgta cttcgcgagc gtcgcggcgg catccttcgc ggcgtattgc ttctgcagcc
    10201 gtgcgagctc tgcatccgcg cgcgctttgc gctcgtcgtc gagctgcttg cgaagctctg
    10261 gcgacggctt cgcggcgatg ccgaacggcg tctcgagcag gccccacgtc ttcagcctct
    10321 cgcgccacgg gttgcccttg ccggctagga tctgctcgac ctgcgcgagc gcggggcgca
    10381 gcgtcagcga cttcttgaag cccttcgact tcgagagctg atgcagatga tcgttccagc
    10441 ccgagcccgt gccgcgaatg ccgaagccgg gcggcgaatc gaggaacttc gcgagccggc
    10501 gccgcagatc gatcacgcga ctctgcacgc gcgcatcgaa tgcaacgagc tcgggatctc
    10561 cgtcgggcag cttcgcgatc cgctcgagct cggccgcgat cagtgcgcgc acgtcgctca
    10621 ccgtcttcgc ctcgagcttg tccgccttca cgccggagag gccgaggtaa acgggctgtc
    10681 cctgatcggt cttcgtgtag gaccacacgt cgctcgcgcc gagatccatc ttgcgcgtct
    10741 tgctgtcgat caactgcttg tagagcgtcg tgctctcgtc gcccgcaaac gaatcgagga
    10801 agagacccat gagcacgcgc tcggtgtcgt cgagatcgcg cgacgcgggc cacgcgaaca
    10861 tcatcggtcc cggattcgtc gcggtcgcat acggatagtc gaccaccttc acggtgcccg
    10921 ccgcggccgg cgtcggcttc ggcagatcgg cctcgctcat cacgcgcccc ttgcgccccg
    10981 cttctttgac gagaatcttc gacgtctcgt cgagcacgct cgcgagtgac atcgacgtcg
    11041 ggaacgccgc gatcatcccc atgttcgcga ggtgatacgc gctctcgtgg aacgcgcgga
    11101 tgtcctgcgg cgtcatgtcg cgaatcgccg gcgggtagcc gcccgagtcg agcacgagcg
    11161 ggtgcttcgg cccgtagaca aggtggcccg cctcgcgcca cagattcacc tcgggcgact
    11221 catacgtgcg caccatctcg ttgtagacgg tgcccttctc ctcgaggtga agcgtgccgt
    11281 catcggcctt gtcgacgccg aagttgcgca cctcgcgccg gatctcttcg tcggagtagt
    11341 cgggattgag cagcgcgtcg agctgatcct cgaacacggg ccagaacacg ctcggccccg
    11401 cgacggtgtg gaagtgatac gcggtgcgcc actgatccgt aaacgcactc gactcggcga
    11461 gcgccatcgc ttcgaagctg ccgagcttgc gcccgcgatc gcctttgccg aggagcaagt
    11521 gctcttgcgt gtgcggctcg cccttatcgc cggtcgggaa cgagttcacc cagatgaagc
    11581 cctgcggagc ggtctcgatc cgcaggtagt cgaacgtgaa gccggtcgcg tcgtgcgtga
    11641 agcgcgcgcc gagaggcttg tccgcatcat cgaggtagag cgcgacggcg gtgaagccgc
    11701 gcaccttcgc gccctcggtc agcgtgtcga gattcgcatc tgcaacgggc gcgggaggag
    11761 cgacgacacc gcctcctcca ccgacggtag ctgtggtggt cttcatgggc tgcggcgggg
    11821 agcaggtggt caagatcaac gcgaacaacg agacgatgcg acgcacgccc ggaaactatc
    11881 acctgcctgc ctacttgacc ggcgacgagg ccggccatct cgcagggcta cgccgtttcg
    11941 cgttcgacga gctcgatcgc acagtccacc gcgagcgaga accggccgcg cctcgtcggc
    12001 acgagctgga tatccgtgca cttttgttcg aggcgcttgc gcaacaggat cagccgcgac
    12061 tcgaggttgt ccttgaacgc cggcaggccg agcgaggggt cgaggcgcag ctcgcgattg
    12121 gagaactcgg tgcgcttctc ggtctcgtac gcgcgaagga tcttccagag gatcttcgcc
    12181 ggcacgttgc ggatgaggta ttcgccgtcg acgaacacac agtcgtcgtt gcggtagtag
    12241 tggaacgtcc gcttgcgcgc gcttgcagcc ggccgcgccg acgcacgcgt caccggcaac
    12301 ggcgcgccgt cgtcctcgcg ctcgagcatg cgatcgatac cgcctgcgat ctgattcgcg
    12361 agcacttgca agaacgcctc gtgccactgc gcgaacgtca tgctgttgcg gcactcgagc
    12421 gcgagcacgg ccacgagacg gtcacctacc atcaagggaa tggacatctg gctctcggcg
    12481 tcgacgaggt gcggcagcgg aatctcctcg ccgagccccg ccttgccggt ctgttcgtac
    12541 tgcgcgcgaa tcgcgcggcc gtagcgcagc tcggagctca cgcagccaat gcgcacgatc
    12601 tggcggccgt cggcaaccga gccgatcacg ccctcgccga tcttcacctc ggcgccgatc
    12661 gcggcgtctc cgtacccatg gctcgcgatc gcgatcaggc gctgtcccgt ttcgtccgga
    12721 accaggatca tgccgtgcga gaacccgaac acttcgtcga gtgcggcgag tgcaccgccg
    12781 agcagcgact cgagatccgt cgcgcgtgcg cagcgctccg agacgagctg cagcccgcga
    12841 agctccgtga gcggtccatc gggcagcatg cgcacgatgc gctctccctg cggtggcggc
    12901 tcgatatagc cctcccgctg ctcgatcgag agcacctcga agacgtccgc actgcgcagc
    12961 ttgaagacgc cggccatgcc ggtgtgaaac gcgatggcct ggatgcgcgt cgacatcgcc
    13021 tcgaacagcg gcccggtcgt ctccgagcgt tcgaacttca ggcgcaggcg gtatgcgtcg
    13081 aacgtgatcg ggtcgtagac ctcgacggtg gcaagcggat tctcgtcgag gttctgcctc
    13141 gtcttgttga agaactggca cgacagcgcg acgtgctgat cgtcgatcag atgcagctcg
    13201 ctcacgtagc tgacgttcgg catcgcgtcg cgatcgcacg tcgcgatcac gccaggaacg
    13261 atgccgtgca gacaacgatc gagatcggag agccggatcg tcacaggctc ttctccgcgg
    13321 acatcggcag cttcacgccg gcgctcgggc cgggcgtctg cgcgaacacg agctcgatcg
    13381 cgagatcgat cgcatgacac ggccagatgc cgaggcggag cgtgttcgcc gccggttgac
    13441 cgacgaatgc gaggtcttca gcaagcagca cgcgatactt ctcggcgagc gcgcgatcgt
    13501 ctgcgttgcc atcgcggatc gcgagcacgt tgcccttcac ctgcacggtc ttgtggctcg
    13561 ggatgtgcga cagcgttacc gcgagacgag gattcgtagc gaggttcgcg atcgactgcg
    13621 cgccggtctt cgccggcatg atgagcgtca ggtgacaggc atcaggccag atgcgcacgc
    13681 ccattccgcg cacgcagtcc ggcacgccat cacgactgca tgttccgacg aggatcgaca
    13741 cgccgccatg cgcgaacgcg acgagctccg gcggtaacgg cgggtacgat gcggacaaga
    13801 cgccgcatct tatcccagcg gtgccccgag cacgagacgc ctctcgaccg ggctgatcga
    13861 tgcttcgaga actcccctcc atgcaagtct ccgttcggcc ggcaggctct cggccatgcg
    13921 catgcgaaga gcgccgagat cgacgtcctt cagccccgcc catagcgttg ccatgatctc
    13981 ggtctcctcg cggttcatgt gcacgagctg caccgagacg aggtgattga cgacgcggat
    14041 gagcttcgcg ccggcctcac cgcgcgccgc gaggtcggct tgctcgagcg cggccgtcgc
    14101 acgctcgacc tcgatctgaa caacctcgag cgcgcgatgt tcggcaccga gctcctccgc
    14161 gagtgccgcg tccagcgccc ggagcgccgg catgacgtag gtctcttcga ttcgcgcgtg
    14221 ctcgtcgaga aactgcagca cggtatcgat tcgcgatgcg aggttgtcga tcgccgccgc
    14281 cgaggtgaga tcgacgcgag ctgcctcgag cgccacctcg aacaagagcg agcgtacgcc
    14341 cttgtggatc atcgagaaca gatcgactcg cattacagcc tcgccgtctt gtcagccgcg
    14401 gcggccggtg ccgacgcctg ttgcggcgga agcggctcgg gtacggcgtt cttgatcggc
    14461 ggcaggctct ggagatacgc gaacaccgcg ccgagctcgt cgtcggtcat gtgcttgtac
    14521 atcgggaacg gcatcggcgg caggagcggt cggccaacgc ccatgtgacg accgttgcgg
    14581 atcgtgtcga tgaacatctg cttcgtccac gacccgaggc cggtctcctt atcgggcgtg
    14641 aggttgcgcg tgaacgacac gccccacggc cccgcccacg cggtgttcgt cgcgagaatg
    14701 ctcgcgaccc aaggaccgct gctcgcaggc ggcgggggca cgatgaagtc gtgcggatgt
    14761 cccgacagct cgcgcgagaa atcgggctcc ggaccgttcg gtcccatctt gagcggcgta
    14821 tggcagtcgt gacacccgct cgtcatcacg aggtgctggc cgcgttcgat ctcggcacgc
    14881 ttcgcaagcg caggatcggc gcctttcgcg ggcgcggagt cgctcttgca ggcggcttgt
    14941 gcaccgacca gcgcggacaa cagaatcatc gtcttcattg cagtcatagg tctctctctt
    15001 tcagttggtt gcgagctggt cgatccaggt cgcgatgaga tgggtcgcct cgtcgtcgac
    15061 gagctgagtt cctagcggag gcatctgcgt gagcgggttg cgcgagcgca tgcgtgcgat
    15121 gacaacgctc agcttggacg cgccgggctg gacgcgcagc gcaggcgcca tgaagcggct
    15181 cggctgaccg acgatggtcc tctcgacacg ccccggctcg acgagcgact gcgagagcac
    15241 catgccgacg gactcgacgg cgccttcgcg gcgatgacag tggccgcagt tgccgtgcag
    15301 gtatcccaag gcagcacgct cgaccggcgc gcccggaatg cggggagcga tattggtgac
    15361 gccgcggatg cgacccgtca tgacgagcga cgcgagatcg agctgcgcgg cctgcgagtg
    15421 cggcgcattc ggatcgcgat cgatcgagag ctgtagtgcc gagaagccga gcacgggcga
    15481 cgaaccgttg ccgtgacacg ccctgcactc gccctcgctc ggcacgcgat gtcgaacccc
    15541 agcggcgagc tcgatcgtcg acgcgccatc cggtgtccgc acagcttcgc cgcccgccca
    15601 cacgtacgtc gcgaattccc aaccatcgcg cgtgcggacc atgaagcgcg tctcgaccgg
    15661 aacgccgccg aacgagaact ccttccacag cttcgtgccg acgggaaact gccacgcgtc
    15721 tggatcgctc gcgtcgatct gcgtgccagg cggcagatag atccatcgtt gcttctcggc
    15781 gccgtcggtc cacagcgggt attgcggcgc gaaggcctcg ttgctcgcgt cgatcgcgtc
    15841 acccgcgtac agcccggtct cgcgcaacgt ttggggtgcg tgcacgagac cgggctcggg
    15901 aagggcccgc tcggtctgcc ggatgagcga gcccgcgacg gccgtcgcga caccagcgcc
    15961 aaccagcgct gtcgcagcga gcattctcga ggccgtcgtt ttcacaccct caagatgcag
    16021 cgtctcgaga aggcgcgttc ctaagtcttc actgagtgtt tcttatgaat tccttgtcga
    16081 ctccctaaag gaaccttaag cgcgtcgctg gtggtatcca cgcgagatgc gctggttcgc
    16141 ttcacttcta ctcgctggct gcatgcattc cgtcgccgcg ccgaccatcc cgccgcatcc
    16201 cggtatggga tccgtccact tcgctatcga cacgcgtgca ccggccgcgc aacagcactt
    16261 cgacgccggc ttagctgcgc tctatggctt caactacgac gaggcgcatt atgaattcct
    16321 ggtcgcttcg cacgccgacc cgagctgcgc gatgtgcgcg tggggcttcg cctacgcgag
    16381 tgggccgaat atcaacgcca tcctgaagca gtatcagggc acctacgaaa gtgcgcagca
    16441 ggcagcgacg ctcgcgagga cgccactgga gaaggcgatc acgaaggcgc tcgtcgcgag
    16501 gctctcgccg cacatgatgg tcagcaccga cgagcgatcg aagctcgacg acgcctacct
    16561 ggcagcgatg cgcgaagccg cgaagctcgc gccctcggat ccggacgtcc aaacgttcct
    16621 cgcggaagcg ctttggatgg caacacccat cggcgttccc ggatatacga aggacgggat
    16681 tgcaacgcat ccgaacgtga ccgaggccgt gagcgttctg gagcacgtgc tcgaagcgca
    16741 tcccgatcac atcggggcca tccacttcta cattcacgcg gtcgacggca gcaaagcgat
    16801 ggagaaggcc gtgcccttcg cgcaaaagct cgcgacgctc gcgccggagt ccggtcacct
    16861 cgtccacatg ccgtcgcatc tcatgctcaa gctcggccga tacgcggaag ccatcgacga
    16921 gaaccgtgca gcaatcgcgt cagacaagcg gtacctcgct cgaaatccga agggcggcga
    16981 gtacgagatg ttctcgatgc atcctcgtca gtacctctgg tacgtgctgc tcttcacagg
    17041 cgggagccgc gaagcaacaa cgctcgcgag cgagctgcgc gatgagatgc agaagatggc
    17101 gatgatggat ccccacgccg cagaccaagc cgcggtgttc gccgcgctag tggctgcgag
    17161 gttcggtaac tgggacacgg cactttcgtt gccgcctcca tcaggttcga tgagcacgat
    17221 cgcgacgagc ttcgctcggg gattggccct cgtagccaaa ggcaagctcg atgaagccgc
    17281 cgtcgaggtc gaaaagatcc gcaaagctcc cgacgcagat acgcctccga gccctccgcc
    17341 cgcgccagcc gcgcacggcg atcacgcgga tacgccggcc gaagcaccgc cgccaatggg
    17401 cggaccgccc gagctcttcg tcaagaagca gcgcgagtac acgcgtgcgc tctcggctgc
    17461 ctttgcggct cagctgtcgg gcgcggttac agcggcgcga ggtaagcagg aagaagccat
    17521 cgccgagctt cgcggcgcgg tcgatctcga ggacgcagtt cctgacatgg gtgagctcag
    17581 caagccgccg gtaaccgctc gccaacgcct cggcgcgctg ctgctcaccg cggggcgccc
    17641 cgccgacgcc gaacgcgtct acctcgccga tctgaaggtc ttcccggaaa acgggtggtc
    17701 tctgttcggt ctctggaaag ctctcgacgc acagcacaag cccgacgccg cagcagcgcg
    17761 agcacgattc gaggcagcgt ggaagcacgc cgacatccag ctcacctcgt cggttttttg
    17821 acgtcattcg gatagacttg gcggcgtgaa gcggggaatc gcaggcgtgg cggcggcggt
    17881 gatgttgtgc gcgacgagct gcgtgcaccg catcgagcgc aagagcggca ccacgatcca
    17941 cacgatcaac gacaaggcgc cgtttctcaa agtgcacatg cccgacggca agctgtacgt
    18001 gctggcggat tggcgcgtcg acgagacgac gaaacacatc actggcaccg gcgacttgtt
    18061 cgacgccgac cgcgagctcg agaagaccgg ctcgttctcg atcgcgatgg acgacgtcgc
    18121 gatgtacgag acgaatacga tcgagtcgtc gcctggcgtc gcggcgatgg ctgtgatctc
    18181 ggggctgtcg gtcgcggtca ccgcgttttg catcgcgaat cccaagtcgt gtttcgggtc
    18241 gtgccccacg ttctacgcgc cgtccgacga caacggccgg cccgtgctgc aagccgaggg
    18301 tttctccgat tcgatcgcgc cctcgctcga gcgcaacgac atcgatgcgc tgtggcagac
    18361 caccggccat gccgggcgca cgacgatcac gatgacgaac gaggcgtacg agacgcacgt
    18421 cgtcaaacaa gtcgatctgc tcgcggtgcc tcgtcccgcg cacggccgcg tcctcgcgac
    18481 cgacgaccac ctctacatcg catcgacgct caccgcgccg acggcgtgca tcgcctccga
    18541 aggctcgtgc ctcgacgcgc tctcgaccgt cgacggccgc gaacgttcga gcacgacgga
    18601 tggcgaagac ctcgcgacgc gcgagacgat ccagctcgcg ttcccgcgcg gcgacggcgc
    18661 gacaggccag cgcgcgatcg tgatcgctgc acgccagagc ctcgtcacga ccttcctcct
    18721 ctatcaaggc ctcgcgtacc tcggcgctga cgcgacacgc tggctcgcaa cgctcggctc
    18781 gatgaatgcc gacgcgcgcg tcggtggccg cgcacttcac cgcctcgtcg gcggcatcga
    18841 ggtccaggtc cgcgacggcg acggctggcg caccgtcgac gaagtctacg agacgggccc
    18901 gatcgcgacc gatgtccacc tcgtcctcct cccggtcgct gcggcaggcg acgagattcg
    18961 tctcgtgctg cagcgcggcg gctggcggat cgacagtgtc gcgctcgcaa cgatcacggg
    19021 cgaagcgcag ccgattcgca tcgcaccgtc gaagatccgc ggcacgctcg gcgctgagta
    19081 cgcgggtgca cgcaagccgg catcacgctt tccgatcatc acgctgccgg gcgatcgcta
    19141 cgagctcgag tacacgctgc ccgcgaccga cgccgcatac gagctcttcc tcgacagtcg
    19201 cggctactac ctcgaatgga tgcgtgacga gtggctcgcc gaccagaaca cgctgtccgc
    19261 gatcaagctg ttcgccgatc cagaagcgat gctgcgcgag ctcgcgcccg catacaaacg
    19321 cgtcgagccg cacatggagg aaatcttctg gaggagccgc tatgcgcgcc cttagcgtcc
    19381 tactcgcgtt cgcgctgacg accgcttgca tctacaaccc cgatccgcgt gagcccacgc
    19441 tcgccgacac gcagcgcaaa ggcttcggcg cgtggatcgt cgtcaccgca cgtgatggca
    19501 gcgagaccca aggcgagctg ctcgcgatcg acaacggctc gctcttcatc aagacgcgcg
    19561 tccgcaagcc ggtcgtcatc ccgctccaca cggtgcaagg cgccgacgtc tacacgtacg
    19621 agagcgattg gggcttcggc gtctggggcc tcctcggcac gctgtcgacg gcgagccacg
    19681 gcctgatcct catcttcagc gcgccgatct ggatgctcag cgcatcgatc gcggccggcg
    19741 tcgagagcgc tcacatccgc ctccaaattc ccgacgacga tatcgacgag atccggaagt
    19801 gggcgcgcta cccgcagggc atgccgaagc aatcgccgac gatctcccgc aaggaagacg
    19861 cgtgggcgtt cacgcagcaa gcgcaagtcg cggcccgcat gggcaactgc gaggccgtca
    19921 ccaagctgga acctcgcgtg ctcgagctcg acgtcggcct ccacgacatg acgttcatgc
    19981 gcgacgaagc gatccggcgc tgcctcggcc tcccctcgct catcgcgccg ccgtcgatgg
    20041 tcccgccaag cacgccgccg gacgctggcg tcacgccgta gctactactc gtcggtgcgc
    20101 agctcgccga ccgaggggtg cagcggtgcg gcatcgagat cgacatcgca gctgacggtc
    20161 agcgtctcgt ccaccgcttg gtcgtcgacc c
//
LOCUS       DATLBZ010000344        14533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4108386, whole genome shotgun sequence.
ACCESSION   DATLBZ010000344 DATLBZ010000000
VERSION     DATLBZ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14533
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4108386"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..489
                     /locus_tag="VMZ53_17790"
     CDS             256..489
                     /locus_tag="VMZ53_17790"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HUS30368.1"
                     /translation="MPKSLAEIPLGATATIKAVEGPRAFRRRLLEMGLVPGIGVRVVT
                     VAPLGDPLQIEVRGGQWSIRRAEAKQIEVEAGR"
     gene            486..2819
                     /gene="feoB"
                     /locus_tag="VMZ53_17795"
     CDS             486..2819
                     /gene="feoB"
                     /locus_tag="VMZ53_17795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HUS30369.1"
                     /translation="MTASSCHDEPAKSELSSSAARAVGTIVPKRPQIVLVGNPNVGKT
                     TLFNALTGLSAKVSNYPGITVERRTGRLALPAGDADLHDLPGTYSLNARSAEEQIAFD
                     AIAGLHGETAPDAAIVCLDATQLARSAYLLLQLRELGTRCVVALTMVDEAGASAPDAK
                     RLRDVLDCEVVPVIAKAKKGLDELVAAVDRVLRSERRSHWVWKPSRSLQDHLDAVRAA
                     LPPGWRAETRFAAADGARPADDKRPDDDKHAETDDTRAADHNRQADDAVALWAMTCIE
                     SNRDEGDELAGVPTDLRAAVAARATSDAIHREIDDAAVLGRWTWLDKELAAHAVRKSA
                     LKAHSRTARIDRILLHRVVGFAVFLGIMTILFMSLFAWADPLIDLIDSAFKSVGAQLR
                     DLLGDGIFTDFLVDGVIAGVGAVMVFLPQILLLFLFLGFLEDCGYLARVAYLMDRIMR
                     SMNLHGRAFVPMLFGFACAVPAIAATRTMERRRDRILTMMVVPLMTCSARLPVYTLLI
                     GALIKGSRITQALLMVGMYLFSASTSLAAAWTMSKVVKPLKAKRLPFIIELPPYRFPR
                     VFDVLRMMWSKSSMFLREAGTVILACSIALWALLYFPRELPKDAPDYAALIEKAPSTD
                     EKHALEDEKQALLLHNSYGGRLGHVIEPVIRPLGFDWKIGIGIIGAFAAREVFVSTLG
                     VVYAVGGESDERSEPLREALRKATKPDGTRAYTPLMALSLMVFFALAMQCMSTLAVVR
                     RETGGFRWPAFLFGYMTVLAWVASFLVYQGGRLLGFG"
     gene            2889..3236
                     /locus_tag="VMZ53_17800"
     CDS             2889..3236
                     /locus_tag="VMZ53_17800"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUS30370.1"
                     /translation="MQYMLLIYGPDQPPTHPEEGRRMMSEYREFTDDIKRTGHFKAGD
                     ALQSVKTATTVRVRDGKVQRTDGPFAETKEQLGGYYLIEAKDLDEATKIAARIPGAKN
                     GSIEVRPIMVFNG"
     gene            complement(3237..5375)
                     /locus_tag="VMZ53_17805"
     CDS             complement(3237..5375)
                     /locus_tag="VMZ53_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30371.1"
                     /translation="MLKRAISIVCVLASIAACGGKKDCPPSKPCGEGTTAPATTAGAP
                     STAKEDKAVDAAAIADAKAKLVAKHGEAHRADIERGVDQVGKLWRVSDGDLTAFVLEQ
                     YLPEQKDRDALFVRLQDMAEQMKGHFLELGRTSRWNTEVDTGPMAAVEPLLAAYDPGA
                     HTTEDMFTTKVAFAALLNFPLTTLADRIKNSDTWSRRQWAEAKLTRKFDTRVPSTLVA
                     AESATEAAADQYIAGYYLWMQHVLAEDGKRLFPAKKHLITHWNLRDELKANYQEKDGL
                     AKQRVIVKVMERIVTQTIPKAVIDNPNLDWNPFTNKVTAAPPDTIEEEGKTKSKEVSD
                     ARENDTRFERVKAHLAAGRAIDPYSPIAPSVIERAFDAAEMPEDRVKTMLVEILESPL
                     AADAAKEMEAKLGRKLEPSDIWYEFGQATGPETELDAITTKKYPTPAAFEKDIPRILR
                     DLGFAPERAKYVAEHITVDGARGAGHAMGAERRGDKAHLRTRVEPSGMTYKGYNIAVH
                     ELGHNVEQTFSLYDIDYTLLSGVPNTAFTEALAFLFQARDLDLLGRPAASGEAERERV
                     LDAYWNTREIAGSALVELEVWHWLYAHPNASAAEIREATVKTAQEIWNKYYAPHLGGK
                     DIPLLGIYSHTITSPLYLFNYVLGHLIAFQVEEHVAGKDKATFAKEFERICRLGSILP
                     DAWMKAATGKPVTTQPLLDATAKALATKKT"
     gene            5410..6645
                     /locus_tag="VMZ53_17810"
     CDS             5410..6645
                     /locus_tag="VMZ53_17810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS30372.1"
                     /translation="MQTVAGIAEVFRAERARVLATVIRSCNNDFELAEEAVQEAFEAA
                     LARWPTEGTPDEPRAWLTQVARFKAIDAIRRRIKHRDVVASEAVAADAPVAASEPELV
                     AVPDDRLRLIFTCCHPALASEAQVALTLRTLCGLTTEEIARAFLIAPTTMAQRLVRAK
                     TKIRDARIPYEIPEASELAERTDAVMAVIYLVFNEGYTASQGDEWIRRDLCREAIRLA
                     RLLVELVPSDKEPKGLLALLLLHDSRRDARTDTQADMVLLEDQDRSKWDRDQIAEALA
                     LIPDALRGGAGPYALQAAIAALHAQAPTAAQTDWPQIAALYAELHRRQPTPIIALNHA
                     VAIAFAEGPAAGLARIDALGAEATELADYHLWHAARADLLRRLGRLSDAAAAYRKALA
                     LVGSDPERRFLERRLAEVS"
     gene            complement(6679..8064)
                     /locus_tag="VMZ53_17815"
     CDS             complement(6679..8064)
                     /locus_tag="VMZ53_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30373.1"
                     /translation="MNTLAVRYATIAAAAIGFAACATSNEPIVIELDSRTDVTRMFDV
                     DGARDVRVVASDPLIGELWLDGGAIAGQGLIDADRWFVPQPIEGERTFVVAASGEVAL
                     RAWGRGAVPPAPTRERSATWFDATLLDDATFVSFARVMSIIAGDGNGGALLERWFETF
                     AAGPGAGRATFAQFLDGVRVEQGVDPRGWDLAKLPFKVTGVHNRIDLARGDVPGAAAR
                     DCGELRVSIASTHPTLAPLHLLFIFRQLPQADDATPDGAIHCRGTARRWAALAGLSPS
                     EFASTARAWLDAAMTRENFALAESVELTISPWQWRQWVPTVGADGAVSLANPPLFQTI
                     DVARVNTPGAIRDAFLVDVAQHRDEILARTWAVPAAFRPRVAEVQPTEKAALVDLSGV
                     DAATGDQVAEALGAIGCPRCHTDNADFVQTSVGRVPSPFYDKELDARTARINALAQGR
                     AVTPAPFGPLQ"
     gene            complement(8327..10321)
                     /locus_tag="VMZ53_17820"
     CDS             complement(8327..10321)
                     /locus_tag="VMZ53_17820"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS30374.1"
                     /translation="MRRTVQGVLLVCVGAALCAALGAASALAATSSRSVPSTFGGNGH
                     NASFDGRLFIVRTGPGWQAYMLRPEAITYESDGLPQANGAMWSDPLQVVAGEPNGENA
                     LAICEPDATRAPYKCDASNNANASGPFACYDVYVFDSDATRSVANGGQVFRRRHLLLR
                     VAEPNTASAHPVEYQWGALESLSIAGGGVLRGIEPTFTRDGRLMVWNGSLNNSDQNSL
                     MMYSVAPSACSLSGWSAPKSLSHMINDPAVNTKYKLAQRQLRAADGTAFSDGAAVYGA
                     YPWLLPEGEAVMFSSALMPCRGPEDPGGCGPRRNATAVLGYPTNWGIANIDGGINPST
                     ADNVRLFFSSPGAQTFSQLPVSQGHDVWPFFGSNTSNYVEVSFDDGLDGKYAGFWHMN
                     ENVTPAGDLDTTKSPDVSGYFNTARTHGAVAFPAANNGVVGKALVATNGWLDVPNDAS
                     LSPVNGITLEMTIKPTQDPNCDGQNNFRWLMRKGEAFGLVLEETRGIRARFRVAGGAI
                     RDLYSGVELPLNTWTKVTAEYDAPSGKMQIRFDDEVVVEQTFAPAQLAGTNDPLTIGG
                     IGTSSSCPSGGNFTGIIDEVSISRVARHIETPLPPDAGVDDGGGSGSGSGSGSNGDAG
                     TGDNNETGAGGGCCDAGGSAPASSALSLGVLVLLRRRRRS"
     gene            complement(10363..11124)
                     /locus_tag="VMZ53_17825"
     CDS             complement(10363..11124)
                     /locus_tag="VMZ53_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30375.1"
                     /translation="MKKRRLVTLALVALAVIAVSVVAVVIISVRMDTGEWRAPKRDDF
                     VRIAHRLDTRPSKTIYLERHSIDLRPGEDDASQGMSSVLASANAGPTKSKAWSGGDAK
                     WKSLVACVKTQFAPFDVEVTDVRPKDTSFLMVVVGGTPADIGIKGSHHVSGLAPFNGS
                     VIPRAVVFGFSATTKNDVRQTCETIAMEVAHAYGLDHEYLCKDVMTYLTGCGTKSFVD
                     ADAPCGESKKRACEGGAKTQNSFRRLADVLGVRAH"
     gene            complement(11134..11991)
                     /locus_tag="VMZ53_17830"
     CDS             complement(11134..11991)
                     /locus_tag="VMZ53_17830"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS30376.1"
                     /translation="MWTSYSARHAAQLIGLPESAVRSCIRDGLVGTPGTVPAQLSFRD
                     LSALRTVKALVDAGLPLPRVRRELLEIQKQLPAGTSLAELTVEARNGQVHVRGTPPAT
                     GTSTEGQLALPFDVTIDPHGAAASVAPAGELRELPRPADGPVPVVVPPATADQWMSRA
                     QLLEERDPVAAIDAYRRSLRLRPDCTEAWINLGRLFAESSDGEAARDCFKSALELDPA
                     EATAYYNLGVLSQDAGKESEAIDLYRRALELDPQLAEAHYNLATLFDQSGDSRSAIRH
                     INEYRKLTR"
     gene            12044..12703
                     /locus_tag="VMZ53_17835"
     CDS             12044..12703
                     /locus_tag="VMZ53_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HUS30377.1"
                     /translation="MTEREIDLEGPLTPGLLVRPRNAKALYVLAHGAGAGMRHAFMRS
                     IAEALADHAIATLRWEFPYMAANKPRPDAPAVAEAAVRAAWTASAKIARSLPRFAGGK
                     SFGGRMTSRAHAASPLPDVRGLIFLGFPLHPPEQPGIERAEHLTSAAGPLLFLQGTRD
                     DLADLKLLRPVVKKLGARATLHIVEHADHGFDVLVRSGRKPADVLDELATAIADWIAR
                     H"
     gene            12724..13506
                     /locus_tag="VMZ53_17840"
     CDS             12724..13506
                     /locus_tag="VMZ53_17840"
                     /inference="COORDINATES: protein motif:HMM:NF020795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HUS30378.1"
                     /translation="MNRGKDNTAADRKRIEQLAKKAGLAAKPILAAAKTAIELVPESL
                     GLKGNAIPTFQQLAAPQSRFGGAPDFHEGDDEWPETYTFVAQVNLGEIGYYVGAEHIP
                     MNGLVSFFVVDGEDDDGDYLGTGYVQHDAYEGNETFWRLPVPKKGVRVLPCSAMTFIA
                     RLTLPHPGTNEAKALKLTADQATAYNDIVYAGYAHPVHQLFGCKNTDLDGDANEVLVL
                     SLASDKKLGMKWGDDNRLNFHIKKTHFAKAKLGEAYPAYVDA"
     gene            13534..>14533
                     /locus_tag="VMZ53_17845"
     CDS             13534..>14533
                     /locus_tag="VMZ53_17845"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS30379.1"
                     /translation="MRVAVVGSGIAGLAAANALLAHRDADVTMFEAAARPGGHVYTVE
                     ANGHAIDMGFIVCNRERYPSFFALLAELGIATRPTTMSFSVVTPRHEWGSASLDAMFA
                     QRRRIFDPRHWGFLGDVVRFLLRAQRDLSTALVQRASLDEYLAERRVSRDVRERFVVP
                     LAAALWSLAPDACGAFPALTYFRFLEQHGMLSPVRPLQWHTVVGGSTRYLDALLGQLR
                     ATKRFTLRLATPVASIVRDARGVTLRVAGAEQRFDRLVVATHANTTLSLLASPTENER
                     RTLGAFRYSTNRTVLHTDRAFLPRRAAAHAAWNYVADPDTARVAVTYSMTKLQGLPD"
BASE COUNT         2424 a         5062 c         4597 g         2450 t
ORIGIN      
        1 atcgcccgct gccacgagat gaccttgatc ggctcgaagc cctggttgag gagaagagtc
       61 tgagcggagt ccatatggga ggggggacgg ccgtgactac agggaacctg gccccatgct
      121 tccaatgtag catcgcgttg ggcgcgtgcg acattccggc actccggtgc gatctcgggc
      181 acatagagcg gcaacctcgg aggcgccacc caaactgaga ttgaaaacga ttttcagaaa
      241 tggtaccgct cgatcatgcc gaagtcgctc gccgagatcc cactgggagc gacggcgacg
      301 atcaaagcag tcgaaggacc tcgcgcattt cggcggcgtt tgctcgagat gggtctcgtg
      361 cccggcatcg gcgtccgggt cgtgaccgtt gcgccgctcg gtgatccact ccagatcgaa
      421 gtccgcggcg gccagtggtc gatccgcaga gccgaagcca aacagatcga agtcgaagcc
      481 ggtcgatgac cgcgtcgagt tgccatgacg agcccgcgaa gagcgagctc tcgagcagcg
      541 ccgcacgcgc agtcggaacg atcgtgccga agcggccgca gatcgtgctc gtcggcaatc
      601 cgaacgtcgg caagaccact ctcttcaacg cgctgaccgg cctgtccgcg aaggtctcga
      661 actatcccgg catcacggtc gagcggcgca ccggccgtct cgcgcttccc gccggcgatg
      721 ccgatctcca cgatctcccg ggcacgtaca gcctcaacgc gcgcagcgcc gaagagcaaa
      781 tcgcgttcga cgcgatcgcc ggccttcacg gcgagacagc gcccgacgcc gcgatcgtgt
      841 gcctcgatgc aacgcagctc gcgcgctcgg cctacttgct gctgcagctg cgcgagctcg
      901 gcacgcgatg cgtcgtcgca ctgacgatgg tcgacgaagc aggcgcgtcc gcgccggatg
      961 cgaagagact acgcgacgtg ctcgactgcg aggtcgtccc ggtcatcgcg aaagcaaaga
     1021 agggcctcga cgagctcgtc gccgcagtcg atcgcgtact gcgctcggag cgtcgctcgc
     1081 actgggtctg gaagccgtcg cgctcgctgc aagaccacct cgacgccgtc cgcgcggcgc
     1141 tgccaccggg ctggcgtgca gagacgcgct tcgctgccgc ggatggcgcg cgccccgccg
     1201 acgacaagcg ccccgacgac gacaagcacg ccgagaccga cgacacgcgc gccgccgacc
     1261 acaaccgaca agccgatgac gccgtcgcgc tgtgggcgat gacctgcatc gagtccaacc
     1321 gcgacgaggg tgatgagctt gccggcgtgc cgaccgatct ccgggcggcg gtggcagcac
     1381 gcgcgacgag cgacgcgatc catcgcgaga tcgacgatgc ggcagtactc gggcggtgga
     1441 cgtggctcga taaagagctc gccgcgcacg cggtcagaaa gtcggcgctc aaggcgcact
     1501 cgcgcaccgc gcgcatcgat cgcatccttc tgcatcgcgt tgtcgggttc gccgtgttcc
     1561 tcgggatcat gacgatcctg ttcatgtcgc tgttcgcgtg ggccgacccg ctcatcgatc
     1621 tcatcgacag tgcgttcaag agcgtgggcg ctcagctgcg cgacctgctc ggcgacggca
     1681 tcttcactga cttcctcgtc gatggcgtga tcgcgggcgt cggtgcggtg atggtgttcc
     1741 tgccgcagat cctgctgctg ttcttgttcc tcggcttcct cgaggactgc ggctacctcg
     1801 cgcgcgtcgc gtacttgatg gatcgcatca tgcgatcgat gaacctgcac ggccgcgcgt
     1861 tcgtgccgat gctgtttggg tttgcgtgtg cagttcccgc gatcgccgca acgcgcacga
     1921 tggagcgccg ccgcgaccgc atcctcacca tgatggtcgt gccgctgatg acgtgctcgg
     1981 cgcgcctgcc cgtctacacg ctgctcatcg gtgcgctgat caaaggctcg cgcatcacgc
     2041 aggcgctgtt gatggtcggc atgtatctgt tcagcgcgtc gacgtcactc gcagctgcgt
     2101 ggacgatgag caaggtcgtc aagccgctca aggcgaagcg cctgccgttc atcatcgagc
     2161 tgccgccgta tcgcttcccg cgcgtgttcg acgtgcttcg catgatgtgg agcaagtcat
     2221 cgatgttctt gcgcgaggcc ggcaccgtga tcctcgcgtg ctcgatcgcg ctgtgggcgc
     2281 tcctctactt cccgcgcgag ctgccgaaag acgcgcccga ctacgccgcg ttgatcgaga
     2341 aggcgccgtc gaccgacgag aaacacgcgc tcgaagacga gaagcaagcg ctgctgctcc
     2401 acaacagcta cggcggccgc ctcggacacg tcatcgagcc cgtcattcgc ccgctcgggt
     2461 tcgactggaa gatcggcatc ggcatcatcg gcgcgttcgc cgcgcgcgag gtgttcgtgt
     2521 cgacgctcgg cgttgtttac gccgtcggtg gcgagagcga cgagcgctcc gagccactgc
     2581 gtgaagcgct ccgcaaggcg acgaagcccg acggcacgcg cgcgtacacg ccgctcatgg
     2641 cgctatcgct gatggtcttc ttcgcactcg caatgcagtg catgagcacg ctcgcagtcg
     2701 tgcgtcgcga gaccggcggg ttccgctggc ccgcgttcct gttcggctac atgaccgtcc
     2761 tcgcgtgggt cgcgagcttc ctggtttacc agggaggtcg cctacttgga ttcggctgaa
     2821 aaaatcttcg ggccgttgtc gatcggcacg tcgctcgttc gacctatggg cgaaaggaag
     2881 aaacgaccat gcaatacatg cttttgatct acgggcccga tcagccaccg acgcatcctg
     2941 aagagggccg acggatgatg tcggagtacc gagaattcac ggacgacatc aagcgcaccg
     3001 gtcacttcaa ggccggcgac gcactgcaga gcgtgaagac cgcgacgacg gtccgcgttc
     3061 gcgacggcaa ggtccagcgc accgacggcc cgttcgccga gaccaaggaa cagctcggcg
     3121 ggtattacct gatcgaggcg aaggatctcg acgaggcgac gaagatcgcc gctcggatcc
     3181 cgggcgcgaa gaacggctcg atcgaagttc gcccgatcat ggtcttcaac ggctgatcaa
     3241 gtcttcttcg tcgcgagcgc cttcgcggtt gcgtcgagga gaggctgcgt cgtgacgggc
     3301 ttgcccgttg cggccttcat ccatgcgtcg gggaggatcg agccgagcct gcagatgcgc
     3361 tcgaactcct tcgcgaacgt cgccttgtct ttgccagcga cgtgctcttc gacctgaaat
     3421 gcgatcaggt ggccgagcac gtagttgaag agatagagcg gcgacgtgat cgtgtggctg
     3481 tagatgccga gcagcgggat gtcttttccg ccgaggtgtg gcgcgtagta cttgttccag
     3541 atctcctgcg cggtcttcac ggtcgcttcg cggatctccg cggcgctcgc gttgggatgc
     3601 gcatagagcc agtgccacac ctcgagctcg acgagcgcgg agcccgcaat ctcgcgggtg
     3661 ttccagtacg catcgaggac acgctcgcgc tccgcttcgc cgctcgcggc gggccggccg
     3721 agcaggtcga ggtcgcgcgc ctgaaacagg aacgcgagtg cctcggtgaa cgcggtgttc
     3781 ggcacgcccg atagcagcgt gtagtcgatg tcgtagagcg agaacgtttg ctcgacgttg
     3841 tggccgagct cgtgcaccgc gatgttgtag cccttgtacg tcatgccgct cggctcgacg
     3901 cgagtgcgca agtgcgcctt gtcgccgcga cgttccgcgc ccatcgcatg gcccgcgccg
     3961 cgcgcgccgt cgacggtgat gtgctcggcg acgtacttcg cgcgctccgg tgcaaagccg
     4021 aggtcacgca agatgcgcgg gatgtctttc tcgaacgctg cgggcgtcgg atatttcttc
     4081 gtcgtgatcg cgtcgagctc ggtctcgggc cccgtcgcct ggccgaactc gtaccagatg
     4141 tcgctcggct cgagcttgcg accgagcttc gcttccatct ccttcgccgc atcggccgcg
     4201 agtggagact cgagaatctc gacgagcatc gtcttcacgc ggtcctcggg catctccgca
     4261 gcgtcgaagg cgcgctcgat cacgctcggc gcgatcggcg aataaggatc gatcgcccgt
     4321 ccggctgcga ggtgagcctt cacccgctcg aagcgcgtgt cgttctcgcg cgcgtcggaa
     4381 acttccttcg acttcgtctt gccctcttct tcgatcgtat cgggcggcgc agccgtcacc
     4441 ttgttcgtga acggattcca gtcgaggttc ggattgtcga tcaccgcttt cggaatcgtc
     4501 tgcgtgacga tccgctccat caccttcacg atcacgcgct gcttcgcgag cccatctttc
     4561 tcttgatagt tcgccttcag ctcgtcgcgc agattccagt gcgtgatgag gtgcttcttc
     4621 gcgggaaaca gccgcttgcc gtcttcggcg agcacgtgct gcatccacag gtagtagccg
     4681 gcgatgtatt gatccgccgc cgcctcggtc gcgctctctg ctgccacgag cgtcgacggc
     4741 acgcgcgtgt cgaacttgcg cgtgagcttc gcttcggccc actggcgccg cgaccacgtg
     4801 tcggaattct tgatgcgatc ggcgagcgtc gtcagcggga aattgagaag cgccgcgaac
     4861 gcgaccttcg tcgtgaacat gtcttcggtc gtgtgcgcgc ccggatcgta tgcagcgagc
     4921 agcggctcca cggcggccat cggtccggtg tcgacttccg tgttccagcg cgacgtccgc
     4981 ccgagctcga ggaagtggcc cttcatctgc tcggccatgt cttgcaggcg cacgaacagc
     5041 gcgtcgcgat ccttctgctc gggcaaatac tgctcgagca cgaacgccgt gaggtcgccg
     5101 tcgctgacgc gccacagctt gccgacttga tcgacgccgc gctcgatatc ggcgcgatgc
     5161 gcctcgccgt gcttcgcgac gagcttcgcc tttgcatccg cgatcgctgc tgcatcgacg
     5221 gccttgtcct ccttcgccgt cgaaggcgcg cccgctgtcg ttgcaggcgc agtcgtcccc
     5281 tctccgcacg gcttgctcgg aggacaatcc ttcttgccgc cgcacgcagc gatcgatgcg
     5341 agaacacaga cgatggaaat ggcgcgcttc aacacgggcg tgtcgtatca caggcagtaa
     5401 gctcgtcgag tgcaaaccgt cgcgggtatc gcggaggtgt tccgcgccga acgcgcacgc
     5461 gtcctcgcga cggtgatccg ctcctgcaac aacgacttcg agctcgccga agaagcggtc
     5521 caagaagcat tcgaagccgc cctcgcccgc tggccgacag aaggcacgcc cgacgaacca
     5581 cgcgcatggc tcacgcaagt cgcgcgattc aaagcgatcg acgcgatccg ccggcgcatc
     5641 aagcatcgcg acgtcgtcgc cagcgaggcc gtcgcagctg acgcacctgt cgcagccagc
     5701 gagcccgagc tcgtcgcggt tcccgatgat cgcttgcgtc tcatcttcac gtgctgtcac
     5761 ccggcactcg catccgaagc gcaagtcgcg ctgacgctgc gcacgctgtg cggcctcacg
     5821 accgaagaaa tcgcgcgcgc gtttctcatt gcaccgacga cgatggcgca gcgcctcgtc
     5881 cgcgcgaaga cgaagatccg cgacgcgcgc atcccgtacg aaatccccga agcgagcgag
     5941 ctcgccgaac gcaccgacgc ggtcatggcc gtcatctatc tcgtcttcaa cgagggctac
     6001 accgcatcgc aaggcgacga gtggatccgc cgcgacctct gccgcgaagc gatccggctc
     6061 gcgcgccttc tcgtcgagct cgtgccgtcc gacaaagagc ccaagggcct gctcgcgctg
     6121 ctgctgctgc acgactcgcg tcgcgacgcg cgcaccgaca cgcaagccga catggtcctc
     6181 ctcgaagacc aagaccgcag caaatgggac cgcgatcaaa tcgccgaagc gctcgcgctg
     6241 atcccggacg cactgcgcgg cggcgctggc ccgtacgcac tgcaagccgc gatcgccgcg
     6301 ctccacgcgc aggcgccgac agccgcgcag accgactggc cacagatcgc cgctctgtac
     6361 gccgagcttc accgccgcca accgacgccg atcatcgcgc tcaaccacgc cgtcgcgatc
     6421 gccttcgccg agggcccagc cgccgggctc gcgcgcatcg acgcactcgg cgccgaggct
     6481 acggagctcg ccgactatca cctctggcac gccgcccgcg ccgacctcct acgccgcctc
     6541 ggccggctct cggacgctgc ggccgcgtac cgcaaagcac tcgcgctcgt cggctcggat
     6601 cccgagcgtc gcttcctcga acgccgcctc gctgaagtga gctagggcgt ccgaccccac
     6661 tgctccaaat tgcagcagct actgcagcgg gccgaacggt gccggcgtta cggcgcggcc
     6721 ctgcgcaagc gcgttaatgc gcgcggtgcg tgcgtcgagc tctttgtcgt agaacgggct
     6781 cggcacgcga ccgacgctcg tctgtacgaa gtccgcgttg tcggtgtgac accgcggaca
     6841 gccaatcgcg ccgagcgcct cggcgacctg atcgcctgtc gcggcatcga cgcccgatag
     6901 atcgacgagt gcggccttct cggtcggctg cacttcagca acgcgcggcc gaaacgcggc
     6961 cgggaccgcc cacgtgcgcg cgagaatctc atcgcgatgc tgcgcgacgt cgacgagaaa
     7021 cgcgtcgcgg atcgcgcccg gcgtgttcac gcgcgcgacg tcgatcgtct gaaagagcgg
     7081 cgggttcgcc agcgacacgg ccccatccgc gccgaccgtg ggaacccact gccgccactg
     7141 ccaaggcgag atcgtgagct cgaccgactc ggcgagcgca aagttctcgc gcgtcatcgc
     7201 cgcgtcgagc cacgcacgcg cagtgctcgc gaattcgctc ggcgacaagc cagcgagcgc
     7261 agcccaccgg cgcgccgtgc cgcgacagtg aatggcgccg tcgggcgtcg cgtcgtcggc
     7321 ttgcggcagc tgccgaaaga tgaacaacaa gtgcagcggc gcgagcgtcg gatgcgttga
     7381 cgcgatcgag acgcggagct cgccgcaatc gcgcgccgcc gcgccgggaa catcgccacg
     7441 cgcgagatcg atgcggttgt gcacgccggt cactttgaac ggcagcttcg cgaggtccca
     7501 cccacgtggg tcgacgccct gctccacacg cacgccgtcg agaaactgcg cgaacgtcgc
     7561 gcgccccgcg cccggccctg ccgcaaacgt ctcgaaccaa cgctcgagta acgcgccgcc
     7621 gttgccatcg ccggcgatga tcgacatgac gcgcgcgaac gacacgaacg tcgcatcgtc
     7681 gaggagcgtc gcgtcgaacc acgtcgcgga tcgttcgcgc gtcggcgcgg gtggcacggc
     7741 gccccgaccc cacgcgcgca acgcgacctc accgctcgcc gcgaccacga acgtgcgctc
     7801 accctcgatc ggttgcggca cgaaccaccg atcggcgtcg atgagcccct ggccggcaat
     7861 cgcaccgccg tcgagccaga gctcgccgat cagcgggtcg ctcgccacga cgcgcacgtc
     7921 gcgcgctcca tcgacatcaa acatccgcgt aacgtccgtg cggctatcga gctcgatgac
     7981 gatcggctcg ttcgaggtcg cacacgccgc gaacccgatc gcagccgctg caatcgtcgc
     8041 gtagcgaacc gctagcgtgt tcaagcgtgc cggctccgtt ccatgcgacg aagctgcgcc
     8101 agcacgttga tgtccagcag ccggacaggg tcaggcacaa gttcacaagt ttcgcggtca
     8161 cgcacgatgt cgcgttccgc gcgcacggtg tcgcgttccg cgcgtacggt gccgcgttcc
     8221 gcgcgtacgg tgcaccgcac ttcgaatgcg cgccgacggc cgcgatgtcg acccgtgggc
     8281 tcgctggctt gcggcggagg cgtcagcgtt gcacggcgtg acgtcctcac gagcggcgcc
     8341 tccggcggag caacacgagc acaccgagcg atagagcgga cgacgccggg gccgagccac
     8401 ctgcatcgca gcatccaccg ccggcgccag tctcgttgtt gtcgcccgta ccggcgtcgc
     8461 cgttcgagcc ggagccggag cccgagcccg agcctccacc gtcgtcgacg cccgcgtcgg
     8521 gcggcagcgg cgtctcgatg tggcgcgcga cgcgtgagat cgagacttcg tcgatgatgc
     8581 ccgtgaagtt gccaccgctc ggacacgagc tcgacgtgcc gattccgccg atcgtgagcg
     8641 gatcgttcgt tccggcaagc tgcgcgggcg cgaatgtctg ctcgacgacg acttcatcgt
     8701 cgaagcggat ctgcatcttg ccgctcggtg cgtcgtattc ggcggtgact ttcgtccacg
     8761 tgttgagcgg cagctcgacg ccggagtaga ggtcgcggat cgcgccaccc gcgacgcgga
     8821 agcgagcgcg aatgccgcgc gtctcctcga gcacgaggcc gaacgcttcg cctttgcgca
     8881 tcagccagcg gaagttgttt tggccgtcgc agtttggatc ttgcgtcggc ttgatcgtca
     8941 tctcgagcgt gatgccgttg acgggcgaga gcgaagcgtc gttgggaacg tcgagccagc
     9001 cgttcgttgc tacgagcgcc ttgccgacga cgccgttgtt cgcggcgggg aatgcgacgg
     9061 cgccgtgcgt gcgcgcggtg ttgaagtagc cgctgacgtc gggtgacttc gtcgtgtcga
     9121 ggtcgcccgc cggcgtgacg ttctcgttca tgtgccagaa gccggcgtat tttccgtcga
     9181 ggccgtcatc gaaggagacc tcgacgtagt tcgacgtgtt cgagccgaag aacggccaga
     9241 cgtcgtggcc ttgcgacacc ggcagctgcg agaacgtttg cgcgccgggc gacgagaaga
     9301 acaggcgcac gttgtcggcg gtcgatgggt tgatgccgcc gtcgatgttc gcgatgcccc
     9361 agttcgttgg atagccgagg acagcggtcg cgttgcgacg cgggccacag ccgcctggat
     9421 cttccgggcc gcggcacggc atcagtgccg acgagaacat gacggcttcg ccctcgggga
     9481 ggagccacgg ataggcaccg tagaccgcgg caccgtcgct gaatgccgtg ccgtcggcgg
     9541 cgcgcagctg acgttgagcg agcttgtact tcgtgttgac cgcggggtcg ttgatcatgt
     9601 gcgagagcga cttcggcgcg gaccaaccac tcaggctgca tgcactcggc gcgacggaat
     9661 acatcatcag gctgttctga tcgctgttgt tcagcgagcc attccagacc atgagacgac
     9721 cgtcgcgcgt aaacgtgggc tcgatgccac gaagtacgcc gccgcccgcg atcgacagcg
     9781 actcgagcgc gccccattga tactcgacgg gatgcgcgct cgcggtgttc ggctcggcga
     9841 cgcgcagcag caagtgccgg cgccggaaga cttgacctcc gttggcgacg gacctcgtcg
     9901 catcggagtc gaagacgtag acgtcgtagc aggcgaacgg accactcgcg ttcgcgttgt
     9961 tcgaggcgtc gcacttgtat ggcgcgcgcg tcgcgtcggg ctcgcagatc gcgagtgcat
    10021 tctcgccgtt cggttctccc gcgacgacct gcagtggatc actccacatc gcgccgtttg
    10081 cttgcggcag gccgtcggat tcgtacgtga tcgcttctgg ccgcagcatg tacgcctgcc
    10141 aaccgggccc ggtgcgcacg atgaagaggc ggccgtcgaa cgacgcgttg tggccgttgc
    10201 cgccgaacgt gctcggcacc gagcgtgagc tcgtcgcagc cagcgcagat gccgcgccta
    10261 gtgccgcgca cagcgcagcg cccacgcaga cgagcaggac accctgaaca gtccttcgca
    10321 tggcgcggac tctagccgcg cacgcgtgtc acgtttgcaa catcagtggg cgcggacgcc
    10381 gagtacgtcg gcgagccggc gaaatgaatt ctgcgtctta gcgccgccct cgcacgcccg
    10441 cttcttcgat tcgccgcacg gcgcgtcggc atcgacaaac gacttcgtcc cgcaacccgt
    10501 caggtacgtc atcacgtctt tgcagagata ttcgtgatcg aggccgtacg catgcgcgac
    10561 ctccatcgcg atcgtctcgc acgtttgccg gacgtcgttc ttcgtcgtgg cggagaagcc
    10621 gaatacgaca gcgcgcggga tcacgctgcc attgaacggt gcgaggcccg acacgtgatg
    10681 cgagcccttg atcccgatgt ccgcgggcgt cccgccgacg acgaccatca gaaagctcgt
    10741 gtctttcgga cgcacgtccg tcacctcgac gtcgaacggc gcgaactgcg tcttcacgca
    10801 cgcgacgagg ctcttccact tcgcgtcgcc gccgctccac gccttcgact tggtcggtcc
    10861 tgcgttcgca cttgcgagca cgctggacat gccctgcgat gcatcatctt cgcccgggcg
    10921 caaatcgatc gaatggcgct ccaggtagat cgtcttcgat ggccgagtgt cgaggcgatg
    10981 cgcgatgcgc acgaagtcgt cgcgcttggg tgcacgccac tcgcccgtat ccatccgaac
    11041 cgagatgatc accaccgcga cgaccgacac cgcgatcacg gcaagcgcca ccagagccag
    11101 cgtgacaagg cgccgcttct tcacgtcgcc attctagcgc gtcagcttcc gatattcgtt
    11161 gatgtgacga atcgcggatc gggaatctcc tgactgatcg aacaacgtcg ccaagttgta
    11221 gtgcgcttcc gcgagttgcg ggtcgagctc gagagcgcgc cgatacagat cgatcgcctc
    11281 cgactccttg cccgcatcct gcgagagcac gccgaggttg taatacgcag tcgcctcggc
    11341 gggatcgagc tcgagtgcgc tcttgaagca atcgcgtgcg gcctcaccgt cgctgctctc
    11401 ggcaaacagc cgccccaagt tgatccacgc ctcggtgcaa tcggggcgca gccgcagcga
    11461 tcgccgatac gcatcgatcg ccgccaccgg atcgcgctct tccagaagct gcgccctcga
    11521 catccactga tccgcagtcg caggcggtac cacgacaggc accggcccat ccgccggccg
    11581 cggtagctcg cgcagctcac ccgcaggcgc cacactcgcc gccgctccgt gcggatcgat
    11641 cgtcacgtcg aacggcaatg cgagctggcc ttccgtcgat gtgcccgttg cgggcggcgt
    11701 accgcgcacg tgcacctggc catttcgcgc ctcgaccgtc agctcggcga gcgacgtccc
    11761 cgccggcagc tgcttctgaa tctcgaggag ctcgcgccgc acgcgcggca gcggcaatcc
    11821 tgcatcgacg agcgccttca cggtccgcag cgccgacaag tcacgaaacg acagctgcgc
    11881 aggcacggtc ccgggcgtac cgacgagccc atcgcgaatg cagctgcgca cggccgactc
    11941 gggcaacccg atcagctgcg cggcatgtcg cgcggagtaa ctcgtccaca cgtcgtcatc
    12001 atcgccgcac cgtcgagcgt gtcaagcgtg cgatggtcac cgcgtgaccg agcgcgagat
    12061 cgatctcgaa ggcccgctga ctcccggcct gctcgtccgc ccgcgcaacg cgaaagcgct
    12121 ctacgtcctc gcgcacggcg cgggcgccgg catgcgccac gcattcatgc gttccatcgc
    12181 cgaagcgctc gccgaccacg cgatcgcaac actgcgctgg gagtttccat acatggccgc
    12241 caacaagccg cggcccgacg caccggccgt cgccgaagct gcagtccgcg cagcctggac
    12301 cgcctcggcc aagattgcgc gctcgcttcc tcgcttcgcc ggcggcaaat cgttcggcgg
    12361 ccgcatgaca tcgcgcgcgc acgccgcctc cccgctgccc gacgtgcgcg gcctgatctt
    12421 cctcggcttc ccgctccacc caccggaaca acccggcatc gagcgcgccg agcacctcac
    12481 gtcggccgca ggcccgctcc tcttcctgca aggcacgcga gacgatctcg cggacctgaa
    12541 gctgctgcgc cccgtcgtca agaagctcgg cgctcgcgcg acgctccaca tcgtcgagca
    12601 cgcggaccac ggcttcgacg tcctcgtccg ctccggccgc aaacccgccg acgtcctcga
    12661 cgagctcgcg accgcgatcg ccgactggat cgcacgccac tgacgcggct atcgtcgcgc
    12721 gtgatgaacc gcgggaagga caacacggcg gccgatcgca agcggatcga gcagctcgcg
    12781 aagaaggctg gcctcgccgc gaaacccatc ctcgccgccg cgaagaccgc gatcgagctc
    12841 gtgccggaat cgctgggcct caagggcaac gcgatcccaa cattccaaca gctcgcggct
    12901 ccccaatctc gcttcggtgg cgcgcccgac ttccacgaag gtgacgacga atggccggag
    12961 acgtacacgt tcgtcgcgca ggtcaatctc ggcgagatcg gctactacgt aggcgccgag
    13021 cacatcccga tgaacgggct cgtgagcttc tttgtcgtcg acggtgagga tgatgacggc
    13081 gattatctcg gcaccggcta cgtccaacac gacgcctacg agggcaacga aacgttctgg
    13141 cgcctcccag tcccgaagaa aggcgtgcgc gtcttgccat gctctgcgat gacgttcatc
    13201 gcgcgcctca cgctgccaca cccgggcacg aacgaagcga aagcgctgaa gctaacagcc
    13261 gaccaagcga ccgcgtacaa cgacatcgtt tacgccggct acgcgcaccc cgtgcatcaa
    13321 ctgttcggct gcaagaacac cgatctcgac ggcgacgcaa atgaagtcct cgtcctcagc
    13381 ctcgcgtcag acaagaagct cggcatgaag tggggcgacg acaaccgcct caacttccac
    13441 atcaagaaga cgcacttcgc gaaagccaag ctcggcgaag cgtacccggc ttacgtcgac
    13501 gcgtagctgc tgtcgtcgtg ttacgactcg cgcatgcgcg tcgctgtcgt cggctcgggc
    13561 atcgcgggtc tcgccgcagc gaatgcgttg ctcgcgcatc gcgacgccga cgtcacgatg
    13621 ttcgaggccg cagcgcgccc cggcggtcac gtctacaccg tcgaagcgaa cggccacgcg
    13681 atcgacatgg gctttatcgt ctgcaaccgc gagcgctatc cgagcttctt cgcgctgctc
    13741 gcggagctgg gcatcgccac gcgcccgacg acgatgtcgt tctccgtcgt gacaccgcgg
    13801 cacgaatggg gcagcgcatc gctcgacgcg atgttcgcgc agcgtcgccg catcttcgat
    13861 ccacgccact ggggcttcct cggcgacgta gtccgcttcc tccttcgcgc gcaacgcgac
    13921 ctctccaccg cgctcgtgca gcgcgcctcg ctcgacgagt acctcgctga acggcgcgtc
    13981 tcgcgcgacg ttcgcgaacg cttcgtcgtg ccgctcgccg ccgcgctgtg gtcgctcgcg
    14041 cccgacgcgt gcggcgcatt ccccgcgctg acgtacttcc gctttctcga gcaacacgga
    14101 atgctgtctc ccgtccgccc actgcagtgg cacacggtcg tcggcggaag cacgcgctac
    14161 ctcgacgcgc tgctcggaca gctgcgcgcg acgaagcgct tcacgttgcg tctggcaacc
    14221 ccggtcgcat cgatcgtgcg cgatgcacgc ggtgtcacgc ttcgtgtcgc cggcgccgag
    14281 caacgcttcg accgcctcgt cgtcgccacg cacgcgaaca ccacgctctc gctcctcgcg
    14341 tcgccgacgg agaacgaacg ccgcactctc ggcgccttcc gctacagcac gaatcgcacg
    14401 gtgctccaca ccgaccgcgc attcctcccg cgccgtgccg ccgcgcatgc agcgtggaat
    14461 tacgtcgccg atccggacac cgcgcgcgtg gccgtcacgt actcgatgac gaagctgcaa
    14521 ggccttcccg acg
//
LOCUS       DATLBZ010000345         9333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1896516, whole genome shotgun sequence.
ACCESSION   DATLBZ010000345 DATLBZ010000000
VERSION     DATLBZ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896516"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1603
                     /locus_tag="VMZ53_17850"
     CDS             110..1603
                     /locus_tag="VMZ53_17850"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HUS30380.1"
                     /translation="MRFVGIDLGTQSLKAVVCDERFEVLGSHAIATTTHYPHAGHAEQ
                     DPHAWEAALAPAISGALAAAKCSAADVTAIGVSGQLDGCVACAADGTAMQAALIWQDK
                     RADARLAIVTPARLFEITGQVADAGHMAPKIRWLKAKGEALTWAPSRYHQPVTFLVER
                     LTGAAVIDPSHASTTMLYDLLAGTWSRELLDAFGIDERELPTIAPATSIAGALTKQGA
                     ALTGLREGTPVAVGTGDDFATPLGAGIVDAGKLIVALGTAEVVGTIASIPVFDRPSAR
                     AETDPWRALDDAMVETHAYPSGGFFIENPGWMSGGVVRWTTQLLGLGSDAELDALAAS
                     APPGAGGVTFVPALTGAMTPVWRSGARGTLHGLGAEHDRSHVARAVLEGLAFACRDVA
                     ARLTALGLPLREAIVLGGGGRSRVWTQIRADALGIPHLVAARADTCPVGAAMIAAVAA
                     GVVPDLRAAAACVPTPQVAALPQRDLDAAYARYHTLVAVLAEHEWPR"
     gene            1621..2019
                     /locus_tag="VMZ53_17855"
     CDS             1621..2019
                     /locus_tag="VMZ53_17855"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HUS30381.1"
                     /translation="MRRMLLVVCVLAGCPSEPPPACKSVEPSCQPAYVPTFPNVYNNT
                     IKATCGTDDSSCHSASGRAGGLSMATMDEAHANLLTLERVKPGDPGCSEMIVRVDSPG
                     KDYQMPPGDPLSPEERCALIQWVQMGAMNQ"
     gene            2019..3470
                     /locus_tag="VMZ53_17860"
     CDS             2019..3470
                     /locus_tag="VMZ53_17860"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HUS30382.1"
                     /translation="MRVLVIVILVGVAACDGCEEKFSVEELQDPETCKECHPKHYDQW
                     SGSMHAYASDDPVFIAMNKRGQRETNGELGDFCIQCHAPMALKLGKASGSNFDPAALS
                     SSTRGITCYFCHNVESIVADHNNGLAIALDQTMRGGAKNPSDSPAHFSEYDTKMASRT
                     NNSEMCGSCHDVTTSAAVHLERTFAEWKTTVFAIQDPANFLPVTCSTCHMTPTDGVIA
                     SGEGLDVKSRELGFHNHTMAAIDIALTPFPRMDEQLMEIQKILKPSIGIKGPRALGAV
                     DARGGICLDPPGILTVRLDSLSPGHHFPSGAAQDRRVWLEVTAYDASDNVLYARGQVP
                     NGADPETQNDASLTCPNGPTTECATFFDKTKKADGSPAHFFWDVATVESNLIKPPITR
                     DPNSSAYDHSSTVTYQVGTLLPQIERIEAKLWVRPLPFGALDELIASGDLDPSVRAAL
                     EKPEATMLAAQSTWLKSTKGTGPFAINTNCNPF"
     gene            complement(3471..3929)
                     /locus_tag="VMZ53_17865"
     CDS             complement(3471..3929)
                     /locus_tag="VMZ53_17865"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HUS30383.1"
                     /translation="MKAIVVAAVGLLAACGGDDGEGDDVDCTMVTGADTFVVGLEKHG
                     TGGTLDFKLMSATPSPPARGDNNWVVQINSMASGVVGAPVDGAMLQVTPFMPAHQHGS
                     PIAVTIAPGTNAGEYNLTPVNLWMPGVWETTIRATSGSTTDSAVYKFCIE"
     gene            complement(4006..4704)
                     /locus_tag="VMZ53_17870"
     CDS             complement(4006..4704)
                     /locus_tag="VMZ53_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30384.1"
                     /translation="MNRDAKTQTSLIALARTRRMQRALAHAYIHRDAPMPTLRTSLGF
                     MTSTSTTTDEVTGVGHQMSLLGVAAAEGVAYVVEELGEWEAPIIYRLWLRGPRAGHLV
                     PIHAWYEQCSDASEIRARIASIATTLERAQNVTPEAWMLSTRIVQRRALRIGTGGEAP
                     IRKFALQLRVEPVSGYGSSGHTTVTAFLRPGAVLREVWSVPGFPGLAIARVTYTGIPN
                     GLGLTKDTMVLLSE"
     gene            complement(4835..6451)
                     /locus_tag="VMZ53_17875"
     CDS             complement(4835..6451)
                     /locus_tag="VMZ53_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30385.1"
                     /translation="MQRLHTLLFVLTATAACGSGGSGPKAAPSGEPSVDKKPTVVTMP
                     ISVTPIDAGVAAPPPPDPDPPKLACESGTSATQAPYPDPVWFCARPDGVKHGPFFTLF
                     PDLQLEIEGHYKDGKLDGVWKRHHHGGAIAEEGAYVAGVPDGEWRQLDETGNVLGTYK
                     LKKGTGKQKKWLDNGPLYSDVTLVKGVPHGPMKIYDSEGHVVVAATLYNGKLDDDHVV
                     GWKNTLRIEEKLHRGTRQGARKIWQFWSLVIDEAYDRGKLDGNFTMWRDDKKKIPRVV
                     GMYDHGKKIGNWIWTDKNNKTEREGSFVDGKKAGVWTEYTDDVVTFTGSFTDGKPDGE
                     FIYSDLKGNELGRFTITAGTGTMQTFHGNKKVASRARMVDGKLAGKYEELTPRGKVIV
                     EGYYSSDKKHGTWREWTESGELVLEQHWKRGKLDGAVKKYVDGKVVSEATYKDGKVEG
                     TYTEYRGSKPSLVGQFADDKRTGTWLAYDNSGAVLLTATYKAGVLDGPWKQASEGVVV
                     EGTMSQGRRSGTWSLTDQAGSVSTTTVKTP"
     gene            6510..7088
                     /locus_tag="VMZ53_17880"
     CDS             6510..7088
                     /locus_tag="VMZ53_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30386.1"
                     /translation="MKTTRLLALSILLGFAFVSTADARPRPHGFNTGGKRFEANKTFG
                     LGLELGVPDGLTGKYFLSSSNAIDFGVGYIYHHYYARDNDGLNIYADYLWHPLVLTSA
                     DAFELPLYVGVGAHFWDWGDYGCDRNGNNCVYYGTSALGVRVPVGIAFDFNNVPLDIF
                     VQLVPTVDFFRHYRDDFGFHVDFSIGIRFWFN"
     gene            complement(7085..7780)
                     /locus_tag="VMZ53_17885"
     CDS             complement(7085..7780)
                     /locus_tag="VMZ53_17885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS30387.1"
                     /translation="MRFLLVLVVVWGTACGGTQTTRRRATDGAITGLTRDGDNAEPLG
                     TAHVRLQGPNAPDAVGVSGRDGMYTIDHVLPGRYTVVGTYAGQTVKTTNVVVDAGEAT
                     YVDILFTPGRPEPYEIDYSEAKLREIKRFRSKDAHTQLEGTLTDAVTHVRIPGAVVTA
                     FGPAGPSADTLQTITGDDGRYKFEQVQPGVYVVSAYYSVGGRGQIEVRRSDIQVESGD
                     DVDVPLFIDTTRR"
     gene            7815..8948
                     /locus_tag="VMZ53_17890"
     CDS             7815..8948
                     /locus_tag="VMZ53_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30388.1"
                     /translation="MVRAVLLGLLTLSAVSCKSHDESQAKSQPGIAAGKVVEVTGSVT
                     LHHGTETRPLAKGDVIDADDVVETGADGSAVIEVAHNNVNWEIGAGKKGTMRESLAWT
                     EPKKDKAAKATEQDSAAAGRHAERSAADNSATAAERESAAEAPAAEAPKAEAAMAPPA
                     DTAAPPPVAAPTAPPVTAAPAAGGGAKKQATRSAPPPPPPPPPGGAAQSESMPAPKPT
                     PPPAPPAKIAAPQAATTSRNAPADMKPSSDDAFGAGGLGLSGTGEGGGGTGDGIGIGT
                     IGHGGGTGTGQGYGAGKGSVTARKAPTAQGLLDAKNAQLKACLKSGTTMHLKIEVDAA
                     GKAKLLFLDKTDWPADTSACVKKIVDGITFPKQASSVTATIKA"
     gene            complement(8938..>9333)
                     /locus_tag="VMZ53_17895"
     CDS             complement(8938..>9333)
                     /locus_tag="VMZ53_17895"
                     /inference="COORDINATES: protein motif:HMM:NF013527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HUS30389.1"
                     /translation="DEPAVVAKHGVLPESIADLLALVGDTADGIPGLEGWGKSSAAKV
                     LTVYKHLEAIPDDPSKWNVPGLRSIDRLAASLREHRAEAALYKKLATLRIDCPITTDA
                     DALAWKGVNRPALDAMLAEIGLPADAIKL"
BASE COUNT         1574 a         3075 c         3028 g         1656 t
ORIGIN      
        1 cgccgcctct tgccacgttg caaccgccgc gtgcccgaag aagttcaccg cctcttgtta
       61 gcatcgttct cgatgctcgt cggaacgatc gacgatgtag cattcgttcg tgcggttcgt
      121 cggtatcgat cttggaacgc agagcctcaa ggcggtcgtc tgcgacgagc gcttcgaagt
      181 cctcggctcg cacgcgattg cgacgacgac gcactatccg cacgcgggcc atgccgagca
      241 ggatccgcac gcgtgggaag ctgccctcgc tcccgcgatc agcggcgcgc tcgccgccgc
      301 aaagtgctcg gcggcggacg tcaccgcgat tggcgtcagc ggccagctcg acggctgcgt
      361 cgcgtgcgcc gccgacggca ccgcgatgca agccgcgctg atctggcagg acaagcgagc
      421 ggacgcgcgc cttgcaatcg tgacacccgc gcggctgttc gagatcaccg ggcaggttgc
      481 cgacgcgggc cacatggcgc cgaagattcg ctggctcaaa gcgaaaggcg aagcgctcac
      541 gtgggcgccg agccgctacc accagccggt tacgtttctc gtcgagcgcc tcaccggcgc
      601 cgcggtcatc gatccgtcgc acgcgtcgac gacaatgctc tacgatctgc tcgccggcac
      661 gtggtcgcgc gagctcctcg acgcattcgg catcgacgag cgcgagctcc cgacgatcgc
      721 accggccacg agcatcgccg gcgcgctcac gaaacaaggc gcggcgctca ccggcctgcg
      781 cgaaggaacg cccgtcgcgg tcggcactgg cgatgacttc gccactccac tcggtgccgg
      841 tatcgtcgac gcaggcaagc tcatcgtcgc gctcggcacc gccgaggttg tggggacgat
      901 cgcgagcata cctgtcttcg atcgtccgtc tgcgcgtgct gagacggatc catggagggc
      961 gcttgacgac gcgatggtcg agacgcacgc gtatccgagc ggcggcttct tcatcgagaa
     1021 tcccggctgg atgagtggcg gcgtcgtgcg gtggaccacg cagctgctcg ggctcggtag
     1081 tgacgccgag ctcgatgcgc ttgccgcgag cgcgccgccc ggtgcggggg gcgtcacgtt
     1141 cgtaccggcg ttgaccggcg cgatgacacc agtgtggcgc tcgggtgcgc gcggcacctt
     1201 gcatgggctt ggcgcggagc atgatcggtc gcacgtcgcg cgagccgtgc tcgaagggtt
     1261 ggcgtttgcg tgtcgcgatg tggccgcgcg gctgacagcg cttggcttac ctctgcgtga
     1321 ggcgatcgtc ctcggcggag gtgggcgcag tcgggtatgg actcagattc gcgccgacgc
     1381 gctcgggatt ccgcatctcg tcgcggcgcg tgcagacacg tgtccggtgg gtgcggcgat
     1441 gattgccgcg gtggctgcgg gcgtggtgcc ggatttgcgc gcagcggccg cgtgcgtccc
     1501 aacgccgcaa gttgcggcct tgccgcagcg agacctcgac gcagcttatg cgcgatatca
     1561 cacgttagtg gccgtcctcg cggaacacga gtggccgcgg taaggtcgcg caacgtggag
     1621 atgcgacgga tgttgctcgt cgtttgcgtg ctcgccggct gtccgagcga gccgccacct
     1681 gcgtgcaagt cggtcgagcc gagctgccag cctgcgtacg tgcccacgtt tccgaacgtc
     1741 tacaacaaca cgatcaaggc gacctgcggc accgacgatt cgtcgtgtca ctcggcgtcg
     1801 gggcgcgcag gtgggctctc gatggcgacg atggacgagg cgcacgcgaa tctcttgacg
     1861 ctcgagcgcg tgaagccggg cgatcctggc tgcagcgaga tgatcgttcg ggtcgactcg
     1921 ccaggtaagg actatcagat gccgccgggc gatccgctgt cgcccgaaga gcgctgcgcg
     1981 ctgatccagt gggtgcagat gggggcgatg aatcaatgat gcgcgtgctc gtaatcgtga
     2041 tcctcgttgg cgtcgccgcg tgcgacggct gcgaggagaa attctccgtc gaggagctcc
     2101 aagacccgga gacgtgcaag gagtgtcatc cgaagcacta cgatcagtgg tcgggctcga
     2161 tgcacgcgta cgcgagcgac gatccggtct tcatcgcgat gaacaagcgc ggccagcgcg
     2221 agacgaacgg cgagctcggc gacttctgca tccagtgcca cgcaccgatg gccctgaagc
     2281 tcggcaaggc atcgggcagc aacttcgatc ccgcagcgct ctcgtcgagc acgcgcggca
     2341 tcacctgcta cttctgccac aacgtcgagt cgatcgtcgc tgaccacaac aacgggctcg
     2401 caattgcgct cgaccagacg atgcgcggcg gtgcgaagaa tccgtccgac agcccggcgc
     2461 acttcagcga gtacgacacg aagatggcga gccggacgaa caactccgag atgtgcggct
     2521 cctgccatga cgtcaccacg tcagccgccg ttcatctcga gcgcacgttc gccgagtgga
     2581 agaccactgt cttcgcgatc caggatccgg caaattttct gcccgtcacc tgcagcacgt
     2641 gccacatgac gccgaccgac ggcgtgatcg ccagcggcga agggctcgac gtgaagtcgc
     2701 gcgagctcgg ctttcacaat cacacgatgg cggccatcga catcgcgctc actccattcc
     2761 cccggatgga cgagcagctc atggagatcc aaaagatctt gaagccgtcg atcggcatca
     2821 agggcccgcg tgcgctcggc gccgtcgatg cgcgcggcgg tatttgcctc gacccgccgg
     2881 gcattctgac cgtgcgtctc gattcgttgt cgcctggcca tcacttcccg agcggcgccg
     2941 ctcaagatcg acgcgtgtgg ctcgaggtca ccgcgtacga cgcgtcggac aacgtgctgt
     3001 acgcgcgcgg ccaagtcccg aacggggccg atccggaaac gcagaacgac gcatcactca
     3061 cgtgtcccaa cgggccgacg acggagtgcg cgacgttctt cgacaagaca aagaaagccg
     3121 acggctcgcc cgcgcacttc ttctgggatg tggcgaccgt cgagagcaac ttgatcaagc
     3181 cgccgatcac gcgcgatccg aactcgtcgg cgtacgatca ctcgtcgacc gtcacgtatc
     3241 aggtcggcac gttgctgccg cagatcgagc ggatcgaagc gaagctctgg gtgcggcctc
     3301 tgccgttcgg cgcgctcgac gagctgatcg catcgggcga cctcgatccg tcggtacgtg
     3361 ctgcgctcga gaagccagag gcgacgatgc tcgcggcgca gagcacgtgg ctcaaatcga
     3421 cgaagggcac cggacccttc gcgatcaaca cgaactgcaa tccgttctag ctactcgatg
     3481 cagaacttgt agacggcgct atcggtagtc gagccgctcg tcgcgcgaat cgtggtctcc
     3541 cacacgcccg gcatccagag gttcaccggc gtgaggttgt attcgccggc gttcgtgccc
     3601 ggcgcgatcg tgaccgcgat gggcgagccg tgttggtgcg ccggcatgaa cggagtgact
     3661 tgcagcatcg cgccgtcgac cggagcgccg actacgccgc tcgccatcga gttgatctgc
     3721 acgacccagt tgttgtcgcc gcgggcgggc ggggagggcg tcgcagacat cagcttgaag
     3781 tcgagcgtgc cgcccgtgcc gtgcttctcg agaccgacga cgaacgtatc ggcgccggtg
     3841 accatcgtgc agtcgacatc gtcgccttcg ccgtcatctc ccccacacgc tgccaacagg
     3901 ccgacggccg ctacgacgat cgctttcatc tctctattac gcacgaggac acgccccagg
     3961 ttacggcata agcctcaagg gcgctaacag cgcgagacgt ctgggctact ccgagaggag
     4021 gaccatcgta tctttcgtga gtcccaggcc attcgggatc cccgtgtacg tcacacgcgc
     4081 gatcgccaat cccgggaagc caggtaccga ccagacctcg cgaagcacgg cgcccgggcg
     4141 caggaacgcc gtcacggtcg tgtggccgct cgagccgtag ccgctcacgg gctcgacgcg
     4201 caactgcagc gcgaacttgc gaatcggtgc ttcgccgccg gtcccgatgc ggagggcgcg
     4261 gcgctggacg atgcgggtcg acagcatcca cgcttcgggc gtcacgttct gcgcacgctc
     4321 gagtgtcgtc gcgatcgacg cgatacgtgc gcgaatctcc gacgcgtccg agcactgctc
     4381 gtaccacgcg tgaatcggca cgagatgtcc ggcgcgcgga ccgcgcagcc aaaggcgata
     4441 gatgatggga gcttcccatt cgccgagctc ttcgacgacg tacgcaacgc cctcagcggc
     4501 cgcgacgccg agcaagctca tctggtggcc cacgccggtc acttcgtccg tcgttgtcga
     4561 cgtcgacgtc atgaagccga gagacgttcg cagcgtcggc atcggtgcat cacgatggat
     4621 gtacgcgtga gcgagcgcgc gctgcatccg acgcgtgcgg gcaagcgcga tcagcgaggt
     4681 ctgggtcttg gcgtcacggt tcacggcggc agttccttct ttccgtatga acgcgcgaga
     4741 cgcccagagg ttgcacccaa gtgatgaatg caacgtgcgg gaataacgtt gtgtctacgt
     4801 cagttgaata gccgccgcgg cacagcgtct gtcgtcacgg tgtctttaca gtcgtcgtgc
     4861 tgacgctacc cgcctggtcg gtcaggctcc acgttccgct gcgacgcccc tgcgacatcg
     4921 tgccttcgac gacaacgcct tcgctggctt gcttccacgg accatcgagc acgccggctt
     4981 tgtacgtggc cgttaacaac acggcgccac tgttgtcgta tgcgagccag gtgccggtgc
     5041 gcttgtcgtc cgcgaactga ccgacgaggg acggcttgct accgcgatac tcggtgtacg
     5101 tgccttcgac cttgccgtct ttgtaggtcg cctcgctgac gaccttgcca tcgacgtact
     5161 tcttgacggc gccatcgagc ttgccgcgct tccaatgttg ctcgagcacg agctcgccac
     5221 tctcggtcca ctcgcgccag gtgccgtgct tcttgtcgga cgagtagtac ccctcgacga
     5281 tgaccttgcc gcgcggcgtg agctcttcgt atttaccggc gagcttgccg tcgaccatgc
     5341 gcgcgcgcga cgcgaccttc ttgttgccat ggaacgtctg catcgttccg gtgcctgcgg
     5401 tgatcgtgaa gcggccgagc tcgttgccct tgagatcgct gtagatgaat tcgccgtccg
     5461 gtttgccatc ggtgaacgag cccgtgaatg tcacgacgtc atcggtgtac tcggtccaca
     5521 cgccagcttt cttgccatcg acgaacgagc cttcgcgctc cgtcttgttg ttcttgtcgg
     5581 tccagatcca gttgccgatc ttcttgccgt ggtcgtacat gccgacgacg cgcgggatct
     5641 tcttcttgtc gtcacgccac atcgtgaagt tgccgtcgag cttgccgcgg tcgtacgcct
     5701 cgtcgatcac gagcgaccag aactgccaga tcttgcgtgc gccctgacgc gtgccgcgat
     5761 ggagcttctc ctcgatccgc agcgtgttct tccaaccgac gacgtgatcg tcatcgagct
     5821 tgccgttgta gagcgttgcc gcaacgacga cgtgaccttc gctgtcgtag atcttcatcg
     5881 gcccgtgcgg cacgcctttg acgagcgtga cgtcgctgta tagcggcccg ttgtcgagcc
     5941 acttcttctg cttgccggtg cccttcttca gcttgtacgt gccgagcacg ttgccggtct
     6001 cgtcgagctg gcgccactca ccgtcgggga cgcccgcgac gtacgcgcct tcttccgcga
     6061 tcgcgccgcc gtgatggtga cgcttccaca cgccgtcgag cttgccgtct ttgtagtggc
     6121 cttcgatctc gagctgcaga tcgggaaaca gcgtgaagaa cggaccgtgc ttcacaccgt
     6181 cgggacgcgc gcagaaccac acgggatcgg gatacggcgc ttgcgtcgcc gacgtgccgc
     6241 tctcgcacgc gagcttcggc ggatcgggat cgggcggcgg tggtgcagcg acgcccgcat
     6301 cgatcggcgt gaccgaaatc ggcatcgtga ccactgtcgg ctttttgtcg acagatggct
     6361 cgccgctcgg tgcagctttc ggaccgctgc ctcccgagcc acaagcagcg gtcgcggtca
     6421 agacgaagag caacgtgtgc aaacgctgca cagttttatt gtgacacgat cacgtagcgc
     6481 ggagcgaaca cgcattaggc tagacgcata tgaagaccac gagacttctc gcgctctcca
     6541 tcctgcttgg ctttgcattc gtctcgactg cggacgcgcg tcctcggcca cacggattca
     6601 acaccggagg aaagcgcttc gaggcgaaca agacgtttgg ccttggcctc gagctcggcg
     6661 tgcccgacgg cctcaccggc aagtacttcc tcagcagcag caacgcgatc gacttcggcg
     6721 ttggctacat ctatcaccac tattacgcgc gcgataacga cggccttaac atctacgccg
     6781 actacctgtg gcatccactc gtcctgacgt cggcggacgc attcgagcta ccgctctacg
     6841 tcggcgtcgg cgctcacttc tgggactggg gcgactacgg ctgcgatcgc aacggcaaca
     6901 actgcgtcta ctacggcacg agcgcgctcg gtgttcgcgt gcctgtcggc atcgcgttcg
     6961 acttcaacaa tgtgccgctc gacatcttcg tccagctcgt tccgacggtg gacttcttcc
     7021 gccattaccg cgacgacttc ggcttccacg tcgatttctc catcggtatc cgcttctggt
     7081 ttaattaacg gcgcgtcgtg tcgatgaaga gcggtacatc gacgtcgtca ccgctttcga
     7141 cctggatgtc gctgcgcctg acttcgatct gaccgcggcc gcccaccgag tagtacgcgc
     7201 tcacgacgta cacgccgggt tgaacctgct cgaacttgta gcggccgtcg tcgccggtga
     7261 tcgtctgcag cgtatcggcg gaaggaccgg caggaccgaa tgccgtgacg acagcgccgg
     7321 ggattcgcac gtgcgtgacc gcgtccgtga gcgtgccctc gagctgcgtg tgcgcgtcct
     7381 tgctgcggaa cctcttgatc tcgcgcagct tcgcttcgct gtaatcgatc tcgtacggct
     7441 ccgggcgacc cggcgtgaac agaatgtcga cgtaggtcgc ttcacccgcg tcgacgacca
     7501 cgttcgtcgt cttgacggtc tggcccgcgt acgtgccgac caccgtgtag cgacccggga
     7561 ggacgtgatc gatcgtgtac atcccgtcgc gccccgagac accgaccgca tccggcgcat
     7621 tgggtccttg caggcgaacg tgcgcggtgc cgagcggttc tgcgttgtca ccgtcgcgag
     7681 tcaggccggt gatcgcgccg tcggtcgcgc gacgacgggt cgtctgcgtg ccgccgcatg
     7741 ccgtgcccca aacaacaacg aggacgagca ggaaccgcac gaccggcact ctggcacgtg
     7801 gcagaatcat ccgcatggtg cgcgcggtgc ttctcggcct tctcacgctg tctgctgtga
     7861 gctgcaagtc tcacgacgag tcgcaggcga agtcgcagcc cggtatcgca gccggcaaag
     7921 tcgtcgaagt cacgggctcg gtgacgctgc accacggtac cgagacgcgc ccgctcgcga
     7981 agggtgacgt gatcgacgcg gacgatgtcg tcgagaccgg cgccgacggc agcgcggtga
     8041 tcgaggtcgc gcacaacaac gtcaattggg agatcggcgc gggcaagaag gggacgatgc
     8101 gcgagtcact cgcgtggacc gagccgaaga aggacaaagc ggccaaggcg acggagcaag
     8161 acagcgcggc cgctggtcgt cacgccgagc gcagcgccgc cgataattca gcgacggccg
     8221 ccgagcgaga gagcgcggcc gaagcgccgg ccgccgaggc gccgaaagca gaggcagcca
     8281 tggcgccgcc cgccgatacc gccgcgcctc caccggttgc tgcaccgacg gcgccgccgg
     8341 taactgcagc acccgcggcc ggcggcggtg cgaaaaaaca ggcgacgcgt tctgctccgc
     8401 cgccgccgcc accacctccg cccggcggcg ccgcgcaatc cgagtcgatg cctgctccga
     8461 agccaacgcc tcctcctgcg ccccccgcga agattgctgc gccgcaagcc gcgacgacat
     8521 cgcggaacgc gcccgccgac atgaagccgt cgagcgacga cgcattcggt gcaggcggtc
     8581 tcggactctc gggcacgggc gaaggcggcg gcggtaccgg cgatgggatc ggcatcggca
     8641 cgatcggtca tggtggcggc acgggaaccg ggcagggcta cggcgctggc aagggatccg
     8701 tcaccgcgcg caaggcgccg acggcgcagg gcctgctcga cgcgaagaac gcgcagctca
     8761 aagcctgctt gaagagcggc acgacgatgc acctgaagat cgaagtcgat gcggccggca
     8821 aagcgaagct gctgttcctc gacaagacgg attggccggc ggacacgtcg gcgtgtgtga
     8881 agaagatcgt cgacggcatc acgttcccga agcaggcctc gagcgtgacc gcgacgatca
     8941 aagcttgatc gcgtcggcgg gcagcccgat ctcggcgagc atcgcgtcga gcgcgggcct
     9001 attcacgcct ttccatgcga gcgcatcggc gtcggtcgtg atcgggcaat cgatgcgcag
     9061 cgtcgcgagc ttcttgtaga gcgccgcctc ggcgcggtgc tcgcgcagcg atgcagcgag
     9121 tcgatcgatg ctgcggaggc cggggacgtt ccacttcgac gggtcgtcgg gaatcgcttc
     9181 gaggtgcttg tagaccgtga gcactttcgc ggcgctgctc ttgccccaac cctcgaggcc
     9241 agggatgccg tccgcggtat ctccgacgag cgcgagcagg tcggcgatcg attccggcag
     9301 cacgccgtgc ttcgcgacga ctgcgggttc gtc
//
LOCUS       DATLBZ010000346         9951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10640300, whole genome shotgun sequence.
ACCESSION   DATLBZ010000346 DATLBZ010000000
VERSION     DATLBZ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10640300"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VMZ53_17900"
     CDS             <1..182
                     /locus_tag="VMZ53_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30390.1"
                     /translation="WVTNQLELGAHVSVFATSENFHDSAHLVHRENTNTDGAIVVNPE
                     TSPHYLLFRFSNQAF"
     gene            complement(179..1228)
                     /locus_tag="VMZ53_17905"
     CDS             complement(179..1228)
                     /locus_tag="VMZ53_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30391.1"
                     /translation="MKTKILALSCLALAACDHWNGSCEDLAEWSIPISRGTSKEGADA
                     NATRAPDGSLFAAFTDDDVTFGTMRVRGPHVAHVSEDGDIIALADASGGGMYPGVMRA
                     TSDRLYVAWGVNPGTLQAYDYDLHSLWSVSLGTSYQQPLDVAATGEVVTVKSTQGPVM
                     TETEILYLNRDGTTRWSLACGTCSLGSIRFTDNADVIAYVYESSMVVRRRLAAADGTV
                     LEEMMVPPTGSPFGNVFLPDGGSLVLNYDHEYIVQRFDAKGVELWSHRYAALALNNPV
                     LSTTGDILLEAAHEGLHGSRYDVLVLDGATGAERATIPSCGYINVLAADAQAYYGLGL
                     TGSDSVGLARFSMTP"
     gene            complement(1225..2208)
                     /locus_tag="VMZ53_17910"
     CDS             complement(1225..2208)
                     /locus_tag="VMZ53_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30392.1"
                     /translation="MRIALLLLFVPTLAFGQPAEDPPVEPSTPTEEAPPPSAPGEPPP
                     TSAQPAPVVTLDETVAPSPPIEITPLVATAVEPIVPAIAPPAPLVMPELVARNRITIS
                     PMVSSILLVNLAMLAVGGTYTDPRLGGLELYGDVKVRAGGVVAIGGSSENEGAGPALG
                     NIRVGARYHVRTGRFRIAPAMWAWLPTSNAEPYNHDAWEGMAGMMDNRGFGRGAGALG
                     VGINAGYFGERRFVQVEAGAAIVSDRGPQIDDVFFAVGYGSQVNDRTSFLTEFRVEAV
                     PEGGRMYGPAIGFGRRDGQATMWRMRFHPLIFPGGELPGLAIGVDVVHRFQ"
     gene            complement(2283..3203)
                     /locus_tag="VMZ53_17915"
     CDS             complement(2283..3203)
                     /locus_tag="VMZ53_17915"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS30393.1"
                     /translation="MLRALLSLTPPAVDSLDAWWAATREHRESATTTADRALVGGVLA
                     DRLGFAFAAGYQEALRALVPGDGVRALCVTEARGNSPKDIDTTLTAEGTNFMVSGAKK
                     WATAGPLASELLVCAKAGVDAQGRNALKMVRVSTTAPGVKITPASAPFVPEVPHAEVV
                     LDRVHIEASAVLPGDGYADYVKPFRTVEDAHVHCAVLGYLVGVARRQELARDLIERLL
                     AAALATRELARADARDPATHIALAGAITLAATLVGEVEVVWEAQAEDREDKQGDAEWS
                     RWQRDRALLKVAGAARAARRDAAWSSVVPT"
     gene            complement(3203..6337)
                     /locus_tag="VMZ53_17920"
     CDS             complement(3203..6337)
                     /locus_tag="VMZ53_17920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30394.1"
                     /translation="MGDTIAASDPDLRPSSSLIGDPAAPPGAIARGNSIGRFVVLGVL
                     GSGGMGVVHSAYDPHLDRKVAIKLLSGAAMRTQDAAIRLLREAQAMAKINHPNVIKVH
                     EVGTHGEQVFIAMEFADAGTLRGWLKEKHSREEILAVFAEAGRGLAAAHSVGLVHRDF
                     KPDNVLMSKDGSIRVTDFGLVSVADAPLPRAATDLPVPTDVKLTDDTPLSQDLTRTGS
                     IMGTPTYMAPEQFSGRASTAQTDQFAFCVALYEALYGERPYAAENYAELSAAVLMGEL
                     RPAPKDTHVPAWMRRALLRGLATDPANRYRSMADLLAALSADPSRRRRKLFAVVASIA
                     FVALGALALFTLRKGANAECRAGGERFDAVWNADKRAKIQSALAATKRPHAAATFERL
                     TKIVDDWGNAWQASYLDACEDTRVRGEQSDRLLDLRMQCLTRRLDDTRATLDLFAAGG
                     PDVADKALSAALALPTVAPCSDAAALTASVPPPETQATRAQVTAVRTELDDARAKRRV
                     ARYADGLGVARDALAKARASNYQPVIAEALLAVGQLESDLADKNSIATLRDAMHAAAS
                     SGDSATMIDASAWLMYVLTNETDQYAVASEVGLLADAMAKHARPPVDVVVRLDNTIGL
                     LQAKQGDVKSAQARYEGALALAQKELGADHPGTLTTLNQLGNLAKQQGRFADARKYLE
                     QVVASRERVLGKDHPDVASALNNLGNVYRVEGKLDDAKQLYERSLAIRIAALGPTHPD
                     VGTSYNNLGTFYSEAGDEVKALEQFDKALAVWEQAYGKDHIELTAALGNIGSSLNARG
                     DRAGARAMFERSRKIIEKAKGPEHPDVAAVLSNLGVVALDENKLDEAFDLFTRAEKIA
                     EKAYGPEHPDVADYLENLTTVYKHQGKLREAKEMTSRSLALIAKAYGQDHTRMGGALV
                     NYASLQIALDDKVGALETFKRSLAIFEAKLGKDHPYVAFALNGIGGTLVELKRNAEAV
                     PYLERALAIRVATEQQASDLAEVRWNLALALIASPKTRARAINEAKTALDDYKKADDA
                     EKVKELTAWLRHPD"
     gene            complement(6449..7912)
                     /locus_tag="VMZ53_17925"
     CDS             complement(6449..7912)
                     /locus_tag="VMZ53_17925"
                     /inference="COORDINATES: protein motif:HMM:NF012851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HUS30395.1"
                     /translation="MAGGQVLEEVTLNWTDLTGAKTGATALGSNKFYRAQIMGDFTVV
                     FTYGRVGQSGQVQKVKGKDLADAQKQLKKKIDSKIAKGYTKVELRSDAEEAKKQAAKT
                     PTAAAAPTTKKTAAKKSSLHPEVDSLLDIIYGSTSKAIASGLSSSAGATKESPLGNLS
                     DAQLDKGADILEEIEQKIGKKAKKADFVELTNEYLSNIPRNIDHARKGKKLDLDSIVI
                     DSKERIQEQRTFIGLLRDAFLQKAVFAEAAAFDDPHEVWYQGLKCDMSWLDPKSDEYK
                     EYKALYDKGQSPINSNFYGKLKVARIWKLEQQGRKTDFDAYANTVAKKPNATGITPGW
                     HGTRTENLMGICKTGLVTPANLPKGVHVTGRAFGLGIYHTPCWPDSGGKTKDEKGKTF
                     TRYNGALKSTNYTSLKGAFYGKNNTADRGYLFLEELALGVPELALEACFDKPAPKKGC
                     DYIYARAHGHASLSNDEVVTFHENASRRVAILEVMHK"
     gene            complement(7913..9421)
                     /locus_tag="VMZ53_17930"
     CDS             complement(7913..9421)
                     /locus_tag="VMZ53_17930"
                     /inference="COORDINATES: protein motif:HMM:NF012851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HUS30396.1"
                     /translation="MVTKASAGSSKKLNIPVDSVCPLQGAKVHEDFDCMLNQTNIGAN
                     NNKFYVIQLLEKGGKYYAWTRWGRVGEEGQNALLGPSDLGKALKAFQGKFKDKTKNAW
                     EERASFKAAPGRYTLIEVDRSADAAKKADEIEEKLKGIDADAAKIQAKTSKKFAPCKL
                     PVETQKFMDLIFDEDMFKGAMASFDIDVKKMPLGQLSKTQVAKGFEVLEELEEAIEAK
                     KGKKIEEVTSRFYTVIPHAFGRRVPPVIDTLELLTKKFEMLNVLGDIEIAQTMQKADA
                     SKAGKLAPHPADENYGKLGAELDVVDSKSKEFDMIQTYIDNTARSGRKPKLTSLFRLD
                     RKGEGKRFDEHDDLDNRKLLWHGTNVAVVAAICKTGLRIMPHSGGRVGKGIYTASENG
                     KSINYMGWASGGHGVMFLAEVALGKEHSITRDDSSLKTAPKGFDSIVARGQKEPDPKE
                     DITFKFDGKDVIVPQGKPIAMKQYAKSSFDQSEYLVYKESQIRLRYALQVKI"
     gene            complement(9568..>9951)
                     /locus_tag="VMZ53_17935"
     CDS             complement(9568..>9951)
                     /locus_tag="VMZ53_17935"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HUS30397.1"
                     /translation="GLPLGDARRAHELAKADLEEVTVDGQPCSCVPVGRRAGEPAVHL
                     LPPYDEYTVAYRDRSAAGTPPTNATTFGESTLLGPNIVVDGIVVGTWRRDRTKIAAKL
                     WSKLPPKDASAVEKAIARYADFVST"
BASE COUNT         1585 a         3245 c         3177 g         1944 t
ORIGIN      
        1 gctgggttac gaatcagctc gagctcggcg cgcacgtctc ggtgttcgcg acgagcgaaa
       61 acttccacga cagcgcgcat ctcgttcatc gcgagaacac gaacaccgac ggcgcgatcg
      121 tggtcaatcc cgagacgtcg ccgcactacc tgctgtttcg cttctcgaac caagccttct
      181 aaggcgtcat cgagaaccgt gcgagtccga cggaatccga gcccgtcagg ccgaggccat
      241 agtaggcctg cgcgtccgcg gcgagcacgt tgatgtaccc gcacgacggg atcgtcgcgc
      301 gctctgcgcc ggtagcaccg tcgaggacga gcacgtcata gcgagaacca tggagacctt
      361 cgtgagccgc ctcgagcaag atgtcgccgg tcgtcgatag aactggattg ttcaaggcga
      421 gcgcggcgta gcgatgcgac cacagctcga cacccttcgc gtcgaaccgc tgcacgatgt
      481 attcgtgatc gtagttgagg acaagtgatc cgccatcggg caggaacacg ttgccaaacg
      541 gggacccggt cggcggcacc atcatttctt cgagcaccgt gccgtccgcc gctgcgagcc
      601 gccggcgcac gaccatcgac gactcgtaga cgtacgcgat cacgtccgcg ttgtccgtaa
      661 accggataga gccgagcgag caggttccac acgccagaga ccaccgcgtc gtgccgtcgc
      721 gattcaaata gaggatctcg gtttcggtca tcaccggacc ttgcgtgctc ttcaccgtca
      781 cgacctcacc ggtcgctgcg acatcgagcg gctgctggta gctggtgccg agcgacacgg
      841 accacagtga gtggaggtcg tagtcgtacg cttgcagcgt ccccgggttc actccccacg
      901 cgacgtacaa ccggtcgctc gtcgcgcgca tcacgccggg gtacatcccg ccgcccgatg
      961 cgtcggcgag cgcgatgatg tcgccatctt cacttacgtg cgcgacgtgc ggtcctctca
     1021 ctcgcatcgt cccgaacgtc acgtcatcgt cggtgaacgc cgcgaacagg ctgccatcgg
     1081 gtgcgcgcgt tgcgtttgcg tcggcgcctt ctttcgacgt gccgcgcgag atcggaatcg
     1141 accattcggc caaatcttcg cagctcccat tccagtgatc gcacgctgcg agcgcgagac
     1201 acgataacgc gaggattttc gtcttcattg gaacctgtgc accacgtcga cgccgatggc
     1261 gagacctggg agctccccgc ccgggaagat gagtgggtga aagcgcatgc gccacatcgt
     1321 tgcctgacca tcgcgccggc caaatccgat cgcgggccca tacatgcggc cgccttccgg
     1381 aaccgcctcg acgcgaaact cggtgagaaa cgacgtccga tcgttcacct gcgagccgta
     1441 cccgacggcg aagaacacgt cgtcgatctg cggcccgcgg tcgctcacga tggcggcgcc
     1501 tgcctcgacc tgcacgaagc gtcgctcgcc gaagtaaccg gcgttgatgc cgacgcctaa
     1561 cgcgcctgca cctcttccga agccgcggtt gtccatcatg ccggccatgc cctcccacgc
     1621 atcgtggttg tacggctcgg cgttggacgt cggaagccat gcccacatcg ccggcgcgat
     1681 gcgaaaccgg cccgtccgaa cgtgataccg cgcgccaaca cggatgttgc cgagtgcggg
     1741 tcctgcgcct tcgttctccg aggagccacc gatcgcgaca acgccgccgg ctcgcacctt
     1801 cacatcgccg tacagctcga gaccgccgag ccgcgggtcc gtgtacgtgc cgccgaccgc
     1861 gagcattgcg aggttgacga gcaagatcga cgacaccatc ggcgagatgg tgatgcgatt
     1921 tctcgcgacg agctcgggca tgacaagcgg ggcgggcggc gcgatcgccg gcacgatcgg
     1981 ctcgaccgcc gtcgcgacga gaggcgtgat ttcgatcggc gggctcggtg cgaccgtctc
     2041 gtcgagggtg acaacagggg ccggttgcgc gctcgttggt ggcggctcac ccggtgcgct
     2101 cggtggcggt gcctcttccg ttggcgtgct cggctcgacc ggcgggtcct ccgcgggctg
     2161 gccaaacgcc agcgtcggga cgaacagcag gagcagcgcg attcgcattc cccataccta
     2221 tcgcgacgcc gcgtgactcg gacacggcat cggcgtgaag aacgcgtgat cggcagagcc
     2281 ttctacgtag gcacgacgct cgaccacgcc gcgtcacggc gagcggcgcg cgcggcaccc
     2341 gcgaccttga gcagcgcgcg atcgcgttgc cagcgcgacc actcggcgtc gccttgcttg
     2401 tcctcgcgat cctccgcctg cgcttcccag accacttcga cctctccgac gagcgttgcc
     2461 gcgagcgtga tcgcaccggc gagcgcgatg tgcgttgcgg gatctctggc gtcggcacgt
     2521 gcgagctcgc gtgtggcaag cgcggcggcg agcagacgtt cgatcagatc gcgggcaagc
     2581 tcttgccggc gcgccactcc gacgagatag ccgagcaccg cgcagtgaac gtgcgcgtct
     2641 tcgacggtac ggaacggctt cacgtagtcc gcgtagccgt cgccgggcag caccgcgctt
     2701 gcctcgatat gcacgcgatc gaggaccact tccgcgtgcg ggacttcggg aacgaacggc
     2761 gcggacgcgg gcgtgatctt tacgccgggc gctgtcgtcg agacgcgcac catcttgagc
     2821 gcgttgcgcc cttgcgcgtc gacgcccgct ttcgcgcaga cgagtagctc gctcgcgagt
     2881 gggccggcgg tcgcccactt cttagcgccg ctgaccatga agttggtccc ttcggcggtg
     2941 agcgtcgtgt cgatgtcttt gggcgagttg cctcgcgctt cggtcacgca tagcgcgcgc
     3001 acgccatcgc ccgggaccag cgcgcgcagc gcctcttgat agcccgccgc gaacgcaaag
     3061 ccgaggcgat cggcgagcac accaccaacg agcgcgcgat cggcagttgt cgtcgcggat
     3121 tctcggtgtt cgcgtgttgc tgcccaccac gcatcgaggg aatcgaccgc gggcggtgtc
     3181 agcgagagca gggcgcgcag catcagtccg ggtgccggag ccaggcggtc agttccttga
     3241 ccttctcggc atcgtcggcc ttcttgtagt cgtcgagcgc ggtcttcgcc tcgttgatcg
     3301 cgcgggcccg cgtttttggt gatgcgatca gcgcgagcgc taggttccac cgaacctcag
     3361 cgaggtcgct ggcttgctgt tctgtagcga cgcggatcgc gagtgcccgc tcgagatacg
     3421 gaaccgcttc ggcgttgcgt ttgagctcga caagcgtgcc accaataccg ttgagcgcga
     3481 acgcgacgta cggatgatcc ttgccgagct tcgcctcgaa gatcgcgagc gagcgcttga
     3541 acgtctcgag cgctccgacc ttgtcgtcga gcgcaatctg caggctggcg tagttcacga
     3601 gcgcgccgcc catgcgtgta tggtcttgtc cgtacgcttt ggcgatcagg gcgagcgatc
     3661 gcgacgtcat ctctttggct tcgcgcagct tgccttggtg cttgtacacg gtggtcaagt
     3721 tctcgaggta atcggcaacg tcggggtgct cgggcccgta cgctttctct gcgatcttct
     3781 cggcgcgcgt gaatagatcg aaggcttcgt cgagcttgtt ctcgtcgagc gcgacgacac
     3841 cgaggttcga cagcacggcc gcgacgtccg ggtgctcggg gcctttggcc ttctcgatga
     3901 tcttgcgtga ccgctcgaac atggcccgcg caccggcgcg gtcgccgcgc gcattgagcg
     3961 agctgccgat gttgccgagc gccgcggtga gctcgatgtg gtccttgccg tacgcctgtt
     4021 cccagacggc gagtgctttg tcgaactgct ccagcgcctt cacctcgtcg ccggcttcgg
     4081 agtagaacgt gcccagattg ttgtacgagg tgccgacgtc gggatgagtg gggccgagtg
     4141 ctgcgatgcg gatcgcgagg gaccgttcgt acaactgttt cgcgtcgtcc agcttgccct
     4201 cgacgcggta gacgttgcca aggttgttga gcgcgctcgc gacgtccgga tgatctttgc
     4261 cgagcacgcg ctcgcggctg gcgacgactt gctcgagata cttgcgtgcg tccgcgaagc
     4321 gaccttgctg cttcgcgaga ttgccgagct gattgagggt cgtcagcgtg ccggggtgat
     4381 ctgcgccgag ctctttctgc gcgagggcga gtgcgccttc gtagcgcgct tgggccgatt
     4441 tgacgtcgcc ttgcttggcc tgcaacagac cgatggtgtt gtcgagccgt acgacgacgt
     4501 cgacgggtgg ccgcgcgtgc ttggccatcg cgtcggcgag cagaccgacc tcgctcgcga
     4561 ccgcgtattg atcggtctcg ttcgtcagca cgtacatcag ccacgccgac gcatcgatca
     4621 tcgtcgcgct gtcgccggag gaagccgcgg cgtgcatcgc atcgcgcagt gtcgcgatgc
     4681 tgttcttgtc ggcgagatcg ctctcgagct gaccaaccgc tagcagggcc tcggcgatca
     4741 ccggctgata gttgctcgcg cgcgcctttg cgagtgcgtc acgtgcgacg ccaagcccgt
     4801 cggcataacg agcgacccgg cgcttggcgc gcgcatcatc gagctctgtt cgcacggccg
     4861 tgacctgcgc gcgcgtcgct tgagtctcgg gaggcgggac cgacgcggtc agcgcagcgg
     4921 catcactgca tggcgcgacc gtgggcaacg cgagcgcggc cgacagcgcc ttgtcggcaa
     4981 catctggccc accggccgcg aagaggtcga gcgttgcccg cgtatcgtcg agtcggcgcg
     5041 tcaggcactg catgcgcaga tcgagcagcc gatccgattg ctcgccgcgc acgcgggtgt
     5101 cttcacacgc atcgagatag gaggcctgcc aagcattgcc ccaatcgtcg acgatcttcg
     5161 tcaggcgctc gaacgtcgcg gcggcatgcg ggcgtttggt cgcggctagc gcggactgga
     5221 tcttcgcgcg cttgtcagcg ttccacacgg cgtcgaaccg ctcaccaccc gcgcggcact
     5281 ccgcgttcgc acccttgcgc agcgtgaaca gcgcgagcgc tccgagtgcg acgaacgcga
     5341 tcgaagcaac gaccgcgaac agcttgcgcc ggcgccggct cggatcagcg gacagcgcag
     5401 caagcagatc cgccatcgag cgatagcgat tcgccggatc ggtcgcgagc ccgcgtagca
     5461 gcgcacgccg catccacgcc ggtacgtgcg tgtctttcgg tgccggccgc agctcgccca
     5521 tgagcaccgc tgccgaaagc tcggcgtagt tctcggcggc atacggccgt tcgccgtaga
     5581 gcgcttcgta gagtgcgaca cagaacgcga actgatcggt ctgcgctgtc gacgcgcgcc
     5641 cgctgaattg ctcgggtgcc atgtacgtgg gcgtgcccat gatcgacccg gtgcgcgtca
     5701 ggtcttgaga cagcggcgtg tcgtcggtca gcttcacgtc ggtcggcacc ggcagatccg
     5761 tcgccgcacg gggcagcggc gcatccgcga cgctcacgag accgaagtcg gtgacgcgaa
     5821 tgctgccgtc cttggacatc agcacgttgt cgggcttgaa gtcgcggtgg acgagaccga
     5881 ccgagtgcgc agcggcgagc ccgcgtcctg cctcggcaaa cacggcgagg atctcttcgc
     5941 gcgagtgctt ttctttgagc cagccgcgca gcgtacccgc gtcggcgaac tccatcgcga
     6001 tgaagacctg ctcgccgtgg gtgccgacct cgtgcacctt gatgacgttc ggatggttga
     6061 tcttcgccat cgcctgcgct tcgcggagca ggcgtatcgc cgcgtcctgg gtgcgcatcg
     6121 cggcacccga gagcagcttg atcgcgacct tgcgatcgag gtgtgggtcg taagcggaat
     6181 gcacgacgcc cattccgccg gagccgagca cgccgagtac gacgaaccgg ccgatgctgt
     6241 tgccgcgcgc gatggctccg gggggagcgg ccggatcccc gatcagcgac gagctcggtc
     6301 gcaaatccgg gtcggatgcc gcgatggtat cgcccattgc ggcatcgcga agcgtttcac
     6361 tacccaggcc tcgttgtgtc ttcatggcaa cacgcgcgag gtgcgctccg cgctccgcac
     6421 cctgggacta accttaccgc gaccggcgct acttgtgcat gacttcgagg atcgcgacgc
     6481 gacgagatgc gttctcgtgg aacgtcacga cctcgtcgtt cgacagcgac gcgtgaccat
     6541 gcgcgcgggc gtagatgtaa tcgcaaccct tcttcggcgc gggcttgtcg aagcacgctt
     6601 cgagcgcgag ctcggggaca ccgagtgcga gctcttcgag gaacaagtag ccgcgatcgg
     6661 ccgtgttgtt cttgccgtag aacgcaccct tgagcgacgt gtagttcgtg ctcttgagcg
     6721 cgccgttgta acgagtgaag gtcttgccct tctcgtcctt cgtcttgcca cccgagtccg
     6781 gccagcacgg cgtgtgatag atgccgagac cgaacgcgcg tcccgtcacg tgcacaccct
     6841 tcggtagatt tgccggcgtc acgagaccgg tcttgcagat acccatgagg ttctcggttc
     6901 gcgtgccgtg ccagccaggc gtgatgcccg tggcattcgg cttctttgcg accgtattcg
     6961 cataggcatc gaagtcggtc ttgcgaccct gctgctcgag cttccagata cgagccacct
     7021 tgagcttgcc gtagaagttc gagttgatcg gagactgacc cttgtcgtag agggccttgt
     7081 actccttgta ctcgtcgctc ttcggatcga gccaagacat gtcgcacttg aggccctggt
     7141 accaaacctc gtgcggatcg tcgaacgcag cagcctcggc gaagacggcc ttctggagga
     7201 atgcatcgcg gagcagaccg atgaacgtcc gctgctcttg gatgcgctcc ttgctgtcga
     7261 tcacgatcga gtcgagatcg agcttcttgc ctttgcgcgc gtgatcgatg ttgcgaggga
     7321 tgttcgagag gtactcgttc gtcagctcga cgaagtcagc cttcttggcc ttcttgccga
     7381 tcttctgttc gatctcctcg aggatatcgg cgcccttgtc gagctgcgcg tccgagaggt
     7441 taccgagcgg cgattccttc gtggcacctg ccgacgacga cagaccactc gcaatcgctt
     7501 tgctcgtcga cccgtagatg atgtcgagca gcgagtcgac ctcgggatgg agcgacgatt
     7561 tcttcgccgc cgtcttcttc gtggtcgggg ccgcggcggc cgtcggggtc ttcgccgctt
     7621 gcttcttcgc ctcttcagcg tcggagcgca gctcgacctt cgtgtagccc ttcgcgatct
     7681 tcgagtcgat cttcttcttc agctgcttct gcgcatcggc gaggtccttg cccttcacct
     7741 tctgcacttg gccagactgc ccgacgcggc cgtacgtgaa gacgaccgtg aagtcgccca
     7801 tgatctgcgc gcgatagaac ttgttcgaac cgagcgcggt cgcaccggtc ttcgcgccgg
     7861 tgagatcggt ccagttgagc gttacttctt cgagaacctg tccgccggcc atttagatct
     7921 tcacctgcag ggcgtagcgg aggcgaatct ggctctcctt gtaaacgagg tactcgctct
     7981 gatcgaagct cgacttcgcg tactgcttca tcgcgatcgg cttgccctgc ggaacgatca
     8041 cgtccttgcc gtcgaacttg aacgtgatgt cctccttcgg atcgggctcc ttctggccgc
     8101 gagccacgat cgagtcgaag cccttcggcg cggtcttgag cgatgaatca tcgcgcgtga
     8161 tcgagtgctc cttaccgagc gcgacttccg cgaggaacat cacgccgtgg ccacccgagg
     8221 cccagcccat gtagttgatc gactttccgt tctccgacgc cgtgtagatg cccttgccga
     8281 cgcgaccacc cgagtgcggc atgatgcgca gaccggtttt gcagatcgct gcgacgacgg
     8341 cgacgttcgt gccgtgccag agcagcttgc ggttgtcgag atcgtcgtgc tcgtcgaagc
     8401 gcttgccttc gcccttgcga tcgagacgga acagcgacgt cagcttcggc ttgcgacccg
     8461 agcgcgccgt gttgtcgatg tacgtctgga tcatgtcgaa ctccttcgac ttggagtcca
     8521 cgacgtcgag ctcggcgccc agcttgccgt agttctcgtc cgccggatgc ggtgcgagct
     8581 tgccagcctt cgaggcgtcg gccttctgca tcgtctgggc gatctcgatg tcaccgagga
     8641 cgttcagcat ctcgaacttc ttcgtcagga gctccagcgt gtcgatcacc ggcggaacgc
     8701 ggcgaccgaa cgcgtgcggg atcacggtgt agaaccgcga cgtcacttct tcgatcttct
     8761 tgcccttctt cgcctcgatc gcttcctcga gctcttcgag gacctcgaag cccttcgcga
     8821 cctgcgtctt cgagagctga ccgagcggca tcttcttgac gtcgatgtcg aacgacgcca
     8881 tcgcgccctt gaacatgtcc tcgtcgaaga tgaggtccat gaacttctgc gtctcgacgg
     8941 gaagcttgca cggcgcgaac ttcttgctcg tcttcgcctg gatcttcgcc gcgtcggcgt
     9001 cgatgccctt gagcttctct tcgatctcgt cggccttctt cgcggcgtcg gcgctgcggt
     9061 cgacttcgat cagcgtgtag cgaccgggcg cagccttgaa gctcgcgcgc tcttcccacg
     9121 cgttcttcgt cttgtccttg aatttgccct ggaacgcttt gagcgccttg ccgagatccg
     9181 acggaccgag cagcgcgttc tggccttctt caccaacgcg accccagcga gtccacgcgt
     9241 aatacttgcc gcccttctcg agcagctgaa tcacgtagaa cttgttgttg ttcgcaccga
     9301 tgtttgtctg gttgagcatg cagtcgaagt cctcgtggac cttcgcgccc tgcagcggac
     9361 aaaccgaatc gacgggaatg ttgagcttct tgctagagcc agccgacgct ttcgtaacca
     9421 ttgtcagaat ccccccaaag aagtagcgac ggtggccatc gtatcagcgc cgtaaaacgc
     9481 gcaaggcaat ccggacgcgc gtgatgcgac gtcgttgcgt ttggcggtgt ccgaatgttt
     9541 ctcgtgggag aggcgcgcac ccgcgtgtca cgtcgacacg aagtcggcgt agcgcgcgat
     9601 agctttctcg acggcagaag cgtccttcgg cggcagtttc gaccacagct tcgcagcgat
     9661 cttggtgcgg tcgcgtcgcc aggtaccgac cacgatcccg tccacgacga tgttcggtcc
     9721 gagcaatgtt gactcgccga acgtggtggc gttcgtcggt ggcgtaccag cagctgagcg
     9781 atcgcgatac gcgacggtgt actcgtcgta cggcggcagc aggtgaacgg caggctcgcc
     9841 ggcccgccgg cccacgggca cacacgagca gggctgcccg tcgacggtga cctcctcgag
     9901 gtcagccttc gcgagctcgt gcgcgcggcg ggcgtcgccc agcggcaggc c
//
LOCUS       DATLBZ010000347         4009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9349014, whole genome shotgun sequence.
ACCESSION   DATLBZ010000347 DATLBZ010000000
VERSION     DATLBZ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9349014"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /gene="alc"
                     /locus_tag="VMZ53_17940"
     CDS             <1..1476
                     /gene="alc"
                     /locus_tag="VMZ53_17940"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HUS30398.1"
                     /translation="WDLPDLASETVGGAAILCNDEFFAEKENLVKPHAAVWKEHEYTD
                     RGKWMDGWETRRFRPVDGVAPGRESDVHDWCIVRLGLPGIIKGVVIDTAFFRGNYPDT
                     AELWGTAVDDPLDLIALNTAKWTPLIPRSPLEGNAQNKFEVRTDQRFTHVRLVIYPDG
                     GVARLRIHGEVSQQLRKTGLVDLAALEHGAVVETCSDMFFGSRNNLIKPGPSKTMADG
                     WETRRRRGPGNDWAIIRLAGPGTIDQLEIDTSHFKGNAPALCTVEGMRDDAWRILLAS
                     PLQPHTKHVFESELRRIGDVAHLRLSVFPCGGVARLRAWGTPALQPDARIAKLNALGE
                     AETLAAFKRCCGSQKWASAMAAARPYEDTAALLRIAERTWWSLTVEDHLEAFAAHPKI
                     GEKKAPDAGDTKWSSGEQAAASTANAQTLAELTEAQRQYESQYGFIFIVCASGRSADA
                     MLTEVRARLASSREVELRTAAEEQAKITRLRLGKLLWELST"
     gene            1473..1805
                     /gene="uraH"
                     /locus_tag="VMZ53_17945"
     CDS             1473..1805
                     /gene="uraH"
                     /locus_tag="VMZ53_17945"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HUS30399.1"
                     /translation="MITTHVLDIAQGRPGKAIAIELERVENGIWHLVGGGITDDDGRL
                     RTLTPQGPVQPGTYRIRFQTGLYFEAQGTPGFFPLVEIQFSVVDGAQHYHVPLLLSPY
                     GYSTYRGS"
     gene            complement(1806..2534)
                     /locus_tag="VMZ53_17950"
     CDS             complement(1806..2534)
                     /locus_tag="VMZ53_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30400.1"
                     /translation="MRAAVAVVLATGCGRVAFDPIADAFTQDAFELCIGGDGTCPLAC
                     VATDADCQTTCGDGRCVGNAGELCGNCTADCATTQLVCGNARCEAGEAADCYADCGPV
                     PWQWNTQETNLFNSVNNARKNGVICPGDTAKRFAPVLTLLTDPVTKNAAREWAWELAH
                     HEFSGDPGQDGETCTNETFAQRRSEGKFTSAVVSFNATGAADAVNRWKGIASVCQIMM
                     SKTVTRGLAGVAIDADPGWVLLMD"
     gene            complement(2546..3256)
                     /locus_tag="VMZ53_17955"
     CDS             complement(2546..3256)
                     /locus_tag="VMZ53_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30401.1"
                     /translation="MRIAVVGLALVGCGRIGFDGASSDAASRDVIVSDTPLDVSEPDA
                     VPAVCGNAVCEGTLGETCGACMSDCAVQTVVCGNGACETSEDGTTCYADCGPTPWPWA
                     QEESDLFSAINSARVAGVQCPGDSAPRFGGTLLLSSAMQAGTREHAWEIAHHEYSQFS
                     GMTCNGRTYAQRQQPYAGNGGLSSTSTTTAFASATLVVDGWKTDATLCPILMTAGYTQ
                     ASVAAAHDGWHGYVAWFK"
     gene            3296..>4009
                     /locus_tag="VMZ53_17960"
     CDS             3296..>4009
                     /locus_tag="VMZ53_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30402.1"
                     /translation="MSAVRTSLPEKKLTKALDRINRAQTRFLRRYPGDTGARQPVHTV
                     YGGAHLFSADTPKKLGELAIKSLDTYAPDAATFAEAIGIRGDLGPKVLERVREKLKRE
                     PVEDFRIDFEDGYGNRSDDEEDGHAIQAAQQVGAGVIAASLPPFIGIRIKPLTRDLAA
                     RSLRTLDIFLTELLTATNGRLPENFVVTLPKIQSRTDVETIVDVFDILEDRLDLEGGA
                     LQLELMVETTQTIFDPGGRS"
BASE COUNT          732 a         1445 c         1194 g          638 t
ORIGIN      
        1 tgggaccttc cggatctcgc gagcgagacc gtcggtggcg cagccatcct gtgcaacgac
       61 gagttcttcg ccgagaagga gaacctcgtg aagccgcacg ccgccgtgtg gaaagagcac
      121 gagtacaccg atcgcggcaa atggatggac ggctgggaga cgcgccggtt tcgccccgtc
      181 gacggcgttg cgccaggccg cgagagcgac gtccacgatt ggtgcatcgt gcgcctcggc
      241 ctgccaggca tcatcaaagg cgtcgtcatc gacacggcgt tcttccgcgg caactatccg
      301 gacaccgccg agctgtgggg aaccgccgtc gacgacccgc tcgatctcat cgcgctcaac
      361 accgcaaagt ggacgcctct cattccgcgc tcgccgctcg aaggcaacgc gcaaaacaag
      421 ttcgaggtgc gcaccgacca acgcttcacg cacgtacgtc tcgtcatcta tcccgatggc
      481 ggcgtcgcac gactgcgcat tcacggcgag gtcagccagc agctgcgcaa gacggggctc
      541 gtcgatctcg cagcgctcga gcacggcgcc gtcgtcgaaa cgtgcagcga tatgttcttc
      601 ggctcgcgca acaatctcat caagccagga ccgtcgaaga cgatggcgga tggctgggaa
      661 acgcggcgcc gtcgcggccc cggcaacgac tgggcgatca ttcgcctcgc gggtcccggc
      721 acgatcgatc agctcgagat cgatacgtcc catttcaagg gcaacgcgcc cgcgctgtgc
      781 accgtcgagg gtatgcgcga cgacgcgtgg cgcatcctgc tcgcatcgcc gctccagcct
      841 cacaccaagc acgtgttcga gagcgagctg cgtcgcattg gtgatgtcgc gcatctccgg
      901 ctctccgtgt tcccgtgcgg cggcgtcgcg cggcttcgcg catggggcac gcctgcgctg
      961 cagcccgacg ctcgcatcgc aaagctcaac gcgctcggcg aagcggagac cctcgctgcc
     1021 ttcaaacgct gctgcggctc gcagaagtgg gcgagcgcga tggcggctgc gcgtccgtac
     1081 gaggacaccg cggcactgct gcgcattgcc gagcgcacgt ggtggtcgct caccgtcgag
     1141 gatcacctcg aagcattcgc ggcgcacccg aagatcggtg agaagaaggc gcccgacgca
     1201 ggagacacga agtggtcgag cggcgagcaa gccgcagcct cgactgcaaa cgcacagacg
     1261 ctcgccgagc tcaccgaagc gcagcgccaa tacgagtcgc agtacggctt catcttcatc
     1321 gtctgcgcat ccggccgcag cgccgacgcg atgctcaccg aagtgcgtgc gcgtctcgca
     1381 agctctcgcg aagtcgagct ccgcaccgcc gccgaagaac aagccaagat cacgcggctt
     1441 cgcctcggca agctgctctg ggagctctcg acatgatcac gacacacgtt ctcgacatcg
     1501 cgcagggccg cccgggcaag gcgatcgcga tcgagctcga gcgcgtcgaa aacggcatat
     1561 ggcacctcgt gggcggcgga atcaccgacg acgacggccg cctccgcacg ctcacgccgc
     1621 aaggtcccgt gcagcccggt acgtatcgca ttcgcttcca gaccggcctc tacttcgaag
     1681 cgcagggcac gcccggcttc ttcccgctcg tcgagatcca gttcagcgta gtcgacggcg
     1741 cgcagcacta tcacgtgccg ctgctcctct cgccgtacgg ctactcgacg tatcgcggat
     1801 cgtgactagt ccatgagcag gacccatccg gggtctgcgt cgatcgcgac gccggcaagt
     1861 ccgcgcgtga cagtcttgct catcatgatc tgacacacac tcgcgatgcc tttccagcga
     1921 ttgaccgcgt cggccgcgcc cgtcgcgttg aacgagacga cagcgctcgt gaactttccc
     1981 tcgctgcgtc gctgcgcgaa cgtctcgttc gtgcaggtct cgccgtcttg accgggatca
     2041 ccgctgaact catggtgcgc cagctcccac gcccattcgc gcgcggcgtt cttcgtcacc
     2101 ggatcggtca ggagcgtcag caccggagca aaccgcttcg ccgtatcgcc gggacatatc
     2161 acgccattct tgcgcgcgtt gttcaccgaa ttgaacaggt tcgtctcctg cgtgttccac
     2221 tgccacggca cgggaccgca gtccgcgtag caatccgccg cctcgcccgc ctcgcaacgc
     2281 gcgttgccgc aaacaagctg cgtcgtcgcg cagtctgccg tgcagttgcc acacagctca
     2341 ccggcgttgc cgacgcatcg accgtcgccg cacgtcgtct ggcagtcggc atccgtcgcg
     2401 acgcacgcaa gagggcaggt gccatcacca ccgatgcaga gctcgaacgc atcctgcgtg
     2461 aacgcgtccg cgatgggatc aaacgctacg cgtccgcatc ccgtcgcgag aaccacggca
     2521 accgcggcgc gcatgctcca cggtatcact tgaaccaagc gacgtagccg tgccagccat
     2581 cgtgagcagc ggcgacggag gcttgcgtgt agccagcggt catgaggatc gggcagagcg
     2641 tcgcgtccgt cttccagcca tcgacgacga gcgtcgcgct agcaaatgcg gtggtcgtcg
     2701 atgtcgacga gagcccaccg ttgcctgcat acggctgctg ccgttgcgca tacgtgcgcc
     2761 cgttgcaggt catgcccgag aactggctgt attcgtgatg cgcgatctcc catgcgtgct
     2821 cgcgcgttcc tgcctgcatc gccgaggaca gcaaaagcgt tccaccgaag cgcggcgcgg
     2881 agtcgcccgg acactgcacg cccgcaacgc gcgcgctgtt gatcgcggag aacaaatcgc
     2941 tctcttcttg cgcccacggc cacggcgtcg gcccgcagtc cgcgtaacac gtcgtgccgt
     3001 cttcgctcgt ctcgcacgca ccgttgccgc agacgaccgt ctgaacggcg cagtcgctca
     3061 tgcatgcacc acacgtctca ccgagcgttc cctcgcacac cgcgttgccg cagaccgccg
     3121 gcacggcatc gggctcgctc acgtccagcg gcgtgtcgct cacgatgaca tcacgtgagg
     3181 ctgcgtcgga cgaggcgccg tcgaagccga tccggccgca tccgacgagt gcgagcccta
     3241 caacggcaat ccgcacatct cgacgatacc gcattgatcg ggctacaacg cagccatgtc
     3301 agctgtccgc acgtcgctcc ccgagaagaa gctcacgaag gccctcgacc gcatcaaccg
     3361 ggcacaaacc cgcttcttgc gccgctaccc cggcgacacc ggcgcgcgcc aaccggtcca
     3421 cacggtctac ggcggcgccc atctcttctc cgcggacacg ccgaagaagc tgggcgagct
     3481 cgcgatcaag tcgctcgaca cctacgcgcc cgacgccgcc acgttcgccg aagcgatcgg
     3541 cattcgcggt gacctcggcc cgaaagtcct cgagcgcgtg cgcgaaaagc tcaagcgcga
     3601 gcccgtcgaa gacttccgca tcgacttcga agacggctac ggcaatcgca gcgacgacga
     3661 agaagacggc cacgcgatcc aagccgcaca acaagtcggc gccggcgtca tcgccgcctc
     3721 actccctcca ttcatcggca ttcgcatcaa gccgctcacg cgtgacctcg ccgcgcgctc
     3781 gctgcgcacg ctcgatatct tcctcaccga gctgctcacc gccaccaacg gccgcctccc
     3841 cgaaaacttc gtcgtcacgc tgccgaagat ccaatcgcgc accgacgtcg aaacgatcgt
     3901 cgacgtgttc gacatcctcg aagatcgcct cgacctcgaa ggcggcgccc tccagctcga
     3961 gctgatggtc gagaccaccc aaaccatctt cgatccaggc ggccgctcg
//
LOCUS       DATLBZ010000348        10524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3198036, whole genome shotgun sequence.
ACCESSION   DATLBZ010000348 DATLBZ010000000
VERSION     DATLBZ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3198036"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VMZ53_17965"
     CDS             complement(<1..912)
                     /locus_tag="VMZ53_17965"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HUS30403.1"
                     /translation="MSREFAFPGTRPRYAPDRVVDIQHIALQLDVDPSKRQVAGIATL
                     RGMVIAPNTRIVELDAVELTIEKVMVSAKSAGWRHDGKRLRIELAAPLPAGAEITLAI
                     EYKGAPRRGLYFIEPDEGYPEKPVQVWSQGQDEDSRYWFPCVDAPSEKATSEVKVTVP
                     ANLFALSNGVLVSDKTSGGKRTLHWRLDVPHSCYLITLAVGDFGTIETKWRDTPVVYY
                     VERGLEEAAERTLARTPQMLELFSQRFGVAYPYPRYAQVFVADFIFGGMENTSATTLT
                     DTVLMDERAAIDYDIDSLVAHELAHQWF"
     gene            complement(924..1871)
                     /locus_tag="VMZ53_17970"
     CDS             complement(924..1871)
                     /locus_tag="VMZ53_17970"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS30404.1"
                     /translation="MRSLLPLLLAGCAGCVGSTPGSNGSVDAPAGGGSSDGSTANPDA
                     PAGGLEAGTLNVSWMHGSQNCATNTDPEVQVHAYNATVHIIRQNKCKTFEAPFIYVIQ
                     GDTKALSIDTGATTTTTLRTTVRGLIGTKQLIAAHSHAHGDHKAGDATFSGQPNTTLV
                     ATSQTAIQQAFGITTWPTSQGTIDLGNRVLDVLAIPGHEAQHIAIYDRKTGILFTGDS
                     LYPGLLFINDWATYRTSMHRLAQFVASRPVSHVLGAHIEMSSTAKQVYPYGTTYQPSE
                     HVLQLGAAHVAELDAALTQLGATPPAQSVAHDDFVIDPQ"
     gene            complement(1956..3173)
                     /locus_tag="VMZ53_17975"
     CDS             complement(1956..3173)
                     /locus_tag="VMZ53_17975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HUS30405.1"
                     /translation="MPFPIKIASRLDPIKPSITLAVTAKAAKLKADGIDVVSFGAGEP
                     DFDTPTHIKDAARTSLDKGVGKYTEVGGVVALRKAIASELSTVHKMTIDPADVLVSSG
                     AKHSLYNLFMALIDPGDEVLIPAPYWVSYPDMVMLAGGRPVILETKAENDFAVTAEQV
                     AAACTGRTRAIVLNNPSNPTGATYTKAQIEALAKVVIEKDLLVISDDIYRQLVYGDAQ
                     YYSIAAVSPEVAKRTILVDGVSKTYAMTGWRIGYTATGKELAPLIKAMAKIQGQSTSN
                     PTHIAQIATIAALTGPQECVGEMRKAFDERRQEMVKLLRAIPGVQCREPKGAFYAFPD
                     VSKYVGKKTPEGSILDDDVQLCDWLVEVGKVAVVPGSGFGAPGFVRLSYATSMDNIRK
                     GVDRLAKALGSLS"
     gene            complement(3176..3673)
                     /gene="coaD"
                     /locus_tag="VMZ53_17980"
     CDS             complement(3176..3673)
                     /gene="coaD"
                     /locus_tag="VMZ53_17980"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HUS30406.1"
                     /translation="MKDQVTTIAVYPGTFDPITNGHLDILERALRLFDHVIVTLAMNQ
                     RKQPLFSAEERMQFIKDAMPQAVDRLSFEQFDGLLVDFCLAHNATVIVRGLRALADFE
                     YEFQFSHMNRRLAPNIDSVFFMTDEKNHYVSSSLVKEVASLGGDVTGLVPAPVVAALK
                     AKYRK"
     gene            complement(3670..4218)
                     /gene="rsmD"
                     /locus_tag="VMZ53_17985"
     CDS             complement(3670..4218)
                     /gene="rsmD"
                     /locus_tag="VMZ53_17985"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HUS30407.1"
                     /translation="MRIVGGDLRGRVLQAPAGMDTRPTSEKVREAIFNILPDVTGLEV
                     LDLFAGSGALGLEALSRGAEFATFVEKSRGAATVLKDNIAKLGLANQTKVVTGDAIAL
                     SAKPLAQRPYMLVFLDPPYKSDLAVRAAKSLSTHRLLTDDAIVVIEHDRIHAPPDQLE
                     SLLRTDERKYGDTMVSFFRVAT"
     gene            4287..5873
                     /locus_tag="VMZ53_17990"
     CDS             4287..5873
                     /locus_tag="VMZ53_17990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HUS30408.1"
                     /translation="MVGNLASWLLDTALANGAGSRVAVREGTRAWTFDQLATISGKTS
                     SALRALKLGRGERVLILMRDTLEAAAAILGTIHAGGVAVPVSELATPDDVQDYVLHAG
                     AGIAVTDGAHEAVLDAIRNETPDLREIVCVDAKLPGSHDFQKIVDAAEPQAAAPSGPE
                     DVCLLLYSAGSGPGELRAVPHCQRTIKAAHESFATQLLELTSKDRILCVARLSTAYGL
                     GSGLFLPLAAQAEALLFPEQPSTAPLLQAIDAFQPTVLFATPSVYAQMAHDAEKEAIA
                     KPLGSLRHAVAGAEGMPERLIPRIRAVLGTEVVVGYGLTEIFQFALAGRSDDPGARPG
                     VCGKPLAGVQVRLVDEDNDTVGPDEIGTLELQCGSLFTGYWSGGGQSGNMELRADGWF
                     TTRDRFMIDREGFYHHCGRVDDLFKVGGKWVSPSEVERALTAHEGVWECAVIGADDDE
                     GLIKPLAFVVTNVGWEGGPKLEGELKEYVKKSLAPYKYPRWIEFVEALPRGPGGKLLR
                     YKLRPQRRRRRAETGSHELP"
     gene            5870..6448
                     /locus_tag="VMZ53_17995"
     CDS             5870..6448
                     /locus_tag="VMZ53_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30409.1"
                     /translation="MTLRVETPSVEIDPAVRARLGVEPILVERKLGRVHPSWLEALDP
                     RMRAALESVSRAALESRTPSAADVWLARWALADLPVDAPAITPLDLAWLVRIGTEQLS
                     LALGRSTRTDMGPRRAAVERCKDAKPDDELSLVRIAARVLAPHLAAHRNARLQLTRAQ
                     PRPIGLVIERELVAHAGTPIDHVPPLSVLRSL"
     gene            6753..8198
                     /locus_tag="VMZ53_18000"
     CDS             6753..8198
                     /locus_tag="VMZ53_18000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HUS30410.1"
                     /translation="MKPGRVIDGDEHDARTLGQQLVNDARREAQDLLDRARADAEKIG
                     AQAAADAATVRQRAGVVTSSETPSGRVDAVTGLVVRATLSAVSLGEIVMIDRPGLSLR
                     AEVVGFSGEQAVLLPLGDLAGIAPASPVWRTGEPLSIRVGDELLGRVLDGVGQPMDGG
                     TAITSGEAWAVDRPAPNALSRPPISAHITTGVRAIDALLTLGRGQRVGLFAAAGVGKS
                     TLLAQIARGTDADVIVLCLVGERGRELAELLDNELAPARAKTVVVCATSDAPPLVRLR
                     AVHTATAIAEWFRDRRGANVLLLVDSLTRVARAQREVGLSAGEPVARHGYPPSVFALL
                     PRLIERTGATREGAITALYTVLVAGNDMDEPIADEVRGLVDGHIVLDRRLAQRGHFPP
                     IDVVASVSRLMPRLVAADHAKAAARVRERLAIYEEHRDLITLGAYQSGRDRKVDDAVS
                     AYPRIEALLRQSRDETAEWDGSLSRLLTING"
     gene            8438..>10524
                     /locus_tag="VMZ53_18005"
     CDS             8438..>10524
                     /locus_tag="VMZ53_18005"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS30411.1"
                     /translation="MTPIGPVPFVGRQSELEEIKQHLAVVPLVSLHGALGSGKTRLVT
                     ELAPMLGAQTTIIECHPGDRASALRARAERALRCVPGTLAQTLTAQARVLVIDDIQHL
                     RIDEATNLLSPLLLGQTALGRVVVVGRDPLSAAVGASIAEIELHGLDVDSASALWASL
                     EEQYGALEAEGTFDTAYGRTRGVPLALRREYARARFGANAWDVPSLDEPSRIAIEALA
                     VLRQPVAPAAVSALAPGVDTEAALTSLVARQLIDAVGDGRLVVHEVVRVDVLSQMAPE
                     ERKRLSASAAELVASTAALSKGPRLAWQAGDDGAFGAMDQVTRVREVVWHWLAAGQRE
                     KAADVLLAHRELAARSGGAGEFEALLEAVSDRPRRRAGTQSDVVPEMPLDEKRSRALA
                     ALRIEIAVRGGRFTEAFERSTAAPGAVSQLIQAELTLASGDASGARRALQGLIGSDDP
                     SERARAIAMLAELELLAGKPDAAAAHLGSLGDVAQADPLARARVHLAAARADEYAGRV
                     AAMRSALARAHGACKSVPACLEASELTAVVDARRAIGLAREGRLVEAATALDAADAAA
                     RDLDAVAVADEVLAARALVARRRGDSAGAAALLHDLVRARRERGDELGALRAELDLAD
                     LEILRGQPALAAELASAALASSVRRELGHLAARGSAVVAAIDLLELRLDAALPRLEEL
                     YGQPALDAASSANV"
BASE COUNT         1639 a         3526 c         3586 g         1773 t
ORIGIN      
        1 gaaccactga tgcgcgagct cgtgtgcgac gagcgaatcg atgtcgtaat cgatcgccgc
       61 gcgttcatcc atcagcacgg tatcggtgag cgtcgtcgcg ctcgtgttct ccatgccgcc
      121 gaagatgaag tcggcgacga agacctgcgc atagcgcgga tacggatacg cgacgccgaa
      181 gcgctgcgag aacagctcga gcatctgcgg cgtccgcgcg agcgtgcgtt cggcagcttc
      241 ttcgaggccg cgctcgacgt aatagacaac cggcgtgtcg cgccacttcg tctcgatcgt
      301 cccgaagtca ccgacggcga gcgtgatcaa gtagcagctg tgcggcacat cgaggcgcca
      361 gtgcagcgtg cgcttgccgc cgctcgtctt gtcggacacg agcacgccat tcgagagcgc
      421 gaacagattc gcggggacgg tgaccttcac ctcgctcgtc gccttttcgc tcggcgcatc
      481 gacgcacgga aaccagtagc ggctgtcctc gtcctggccc tgcgaccaga cctgcacggg
      541 cttctcgggg tagccctcat caggctcgat gaagtagaga ccgcggcgcg gcgcgccctt
      601 gtactcgatc gcgagcgtga tctcggcgcc ggcaggaagc ggtgcggcga gctcgatgcg
      661 caatcgcttg ccgtcgtggc gccagcccgc cgacttcgcg ctgaccatca ccttctcgat
      721 cgtcagctcc acggcgtcga gctcgacgat gcgcgtgttc ggtgcgatca ccatgccgcg
      781 cagcgtcgcg atgccggcca cctggcgctt gctcggatcg acatcgagct gcagcgcgat
      841 gtgctggata tcgacgacgc gatccggggc gtagcggggg cgggttccgg gaaatgcaaa
      901 ctcgcgggac atgcccgcgg acgttactgc ggatcgatga cgaagtcgtc gtgcgcgacg
      961 gattgcgccg gcggtgtcgc gcccagctgt gtcagtgcag cgtcgagctc ggccacatgc
     1021 gccgcgccga gctgcagcac gtgctccgag ggctgatacg tcgtgccgta tgggtagacc
     1081 tgcttggccg tcgacgacat ctcgatgtgt gcgccgagga cgtggctcac cggacgcgac
     1141 gcgacgaact gcgcgagccg atgcatgctc gttcgatacg tcgcccagtc gttgatgaag
     1201 agcaggccgg gatagagcga gtcgccggtg aacaagatgc cggtcttgcg atcgtagatc
     1261 gcgatgtgct gcgcctcgtg gccgggaatc gcgaggacgt cgagcacgcg gtttccgaga
     1321 tcgatcgtgc cctgcgatgt cggccacgtc gtgatgccga aggcttgttg gatcgcggtc
     1381 tggctcgtcg cgacgagcgt cgtgttcggc tgaccggaga acgtcgcgtc gcccgcttta
     1441 tgatcgccat gcgcgtgcga gtgcgcggcg atgagctgct tcgtgccgat cagaccccgt
     1501 accgtcgtgc gcagcgtcgt ggtcgttgtc gcgccggtgt cgatcgacag tgctttcgtg
     1561 tcgccttgga tcacgtagat gaacggcgcc tcgaatgtct tgcacttgtt ctggcggatg
     1621 atgtgaaccg tcgcgttgta cgcgtggacc tgaacttcgg gatcggtgtt cgtcgcgcag
     1681 ttctgcgagc cgtgcatcca gctgacgttc agcgttcccg cctcgaggcc accggcgggt
     1741 gcatcggggt tcgccgtcga gccatcgctg ctgccgccgc cagcgggcgc gtcgacagaa
     1801 ccgttcgagc caggcgtcga accgacgcaa ccggcgcaac cggcgaggag caggggcaag
     1861 agcgagcgca cggccaacat agaagcagat atcgcgccgc tcggtgggcg taatcatccc
     1921 gccaggttgc gacgcgcgcg ctgggactcg aggccctacg agagcgagcc gagcgccttc
     1981 gcgagccggt cgacaccctt gcggatgttg tccatcgacg tcgcgtacga caggcgcacg
     2041 aagccaggcg cgccgaagcc ggaaccgggc acgacggcga ctttgccgac ttcgacgagc
     2101 cagtcgcaga gctgcacatc gtcgtcgagg atcgagccct cgggcgtctt cttgccgacg
     2161 tacttcgaga cgtcggggaa tgcgtagaac gcgcccttcg gctcgcggca ctgcacgccc
     2221 ggaatcgcgc gcaggagctt caccatctct tggcggcgct cgtcgaatgc tttgcgcatc
     2281 tcgccgacgc actcttgcgg tcccgtgagc gcagcgatcg tcgcgatttg cgcgatgtgc
     2341 gtcgggttcg atgtggactg gccttggatc ttcgccatcg ctttgatgag cggcgcgagc
     2401 tccttgccgg tggcggtgta gccgatgcgc cagcccgtca tcgcgtacgt cttgctaacg
     2461 ccgtcgacga ggatcgtgcg cttcgcgacc tcgggagaga ccgcggcgat cgagtagtac
     2521 tgcgcatctc cgtagacgag ctggcgatag atatcgtcgg agatgacgag caggtccttc
     2581 tcgatgacga ccttcgcgag cgcttcgatc tgcgccttcg tgtacgtcgc gccggtcggg
     2641 ttcgacgggt tgttgagcac gatcgcgcgc gtgcgaccgg tgcatgcggc ggcgacttgc
     2701 tccgcggtca cggcgaagtc gttctccgct ttcgtctcga gaatgacggg acggccaccg
     2761 gcgagcatca ccatgtcggg gtagctcacc cagtacggcg cggggatgag cacttcatcg
     2821 cccggatcga tcagcgccat gaacaagttg tagagcgagt gcttcgcgcc cgacgacacc
     2881 agcacgtccg ccggatcgat cgtcatcttg tggacggtcg agagctcact cgcgatcgcc
     2941 ttgcgcagcg caacgacgcc accgacctcg gtgtacttgc cgacgccctt gtcgagcgac
     3001 gtgcgcgccg cgtctttgat gtgcgtgggc gtgtcgaagt cgggctcgcc ggcgccgaag
     3061 ctgacgacgt cgatgccgtc ggccttgagc ttggcggcct tcgccgtgac ggcgagcgtg
     3121 atcgacggct tgatcggatc gaggcgagac gcaatcttga tcggaaaggg catggctact
     3181 ttctgtactt cgccttcaac gcggcgacca cgggagcagg gacaagaccg gtgacatcgc
     3241 caccaaggct tgcgacctct ttgacgagcg agctcgagac gtagtgattc ttctcgtcgg
     3301 tcatgaagaa gaccgagtcg atgttgggcg cgagccggcg gttcatgtgc gagaactgga
     3361 actcgtactc gaagtcggcg agggcgcgca ggccgcggac gatgacggtc gcattgtgcg
     3421 cgaggcagaa gtcgacgagc aggccatcga actgctcgaa cgagaggcgg tcgaccgcct
     3481 gcggcatcgc atctttgatg aactgcatcc gctcttcggc agagaacagc ggctgcttgc
     3541 gctggttcat cgcgagcgtg acgatcacgt ggtcgaacag ccgcagcgcg cgctcgagga
     3601 tatcgaggtg accgttcgta atcggatcga acgttcctgg atacacggcg atcgtcgtga
     3661 cttggtcttt catgtcgcca cccggaagaa ggagaccatc gtgtcgccgt acttgcgctc
     3721 gtccgttcgt agcagagatt cgagctggtc gggaggcgcg tggatgcgat cgtgctcgat
     3781 gacgacgatc gcgtcgtcgg tcaataaccg atgcgtcgac agtgacttcg ccgcacggac
     3841 ggcgagatcc gacttgtaag gcggatcgag gaacacgagc atgtacggtc gctgcgccaa
     3901 cggcttggcc gagagcgcga tcgcatcacc ggtcacaacc ttggtctggt tcgcgagacc
     3961 gagcttggcg atgttgtcct tcaggaccgt cgcggcacct cgcgacttct cgacaaacgt
     4021 ggcgaattca gcgccgcgtg aaagcgcttc gagcccgagc gcgcccgagc cggcgaacag
     4081 gtcgagcacc tcgagcccgg tcacatccgg gagaatgttg aagatcgcct ccctgacttt
     4141 ctccgacgtg gggcgcgtat ccatgcccgc aggggcctgc aggaccctgc cgcgcaggtc
     4201 gcctccgacg atccgcatct ccccaggaat gtacgacttc tttttgttat gggcccgtac
     4261 ctccgatacc attacagcgc acgaccatgg tcgggaacct ggcctcttgg ctgctcgaca
     4321 ccgcgctcgc gaatggcgcg ggatcgcgcg tggctgttcg cgagggcacg cgtgcgtgga
     4381 cgttcgatca gctcgcgacg atttcgggca agacatcgtc cgcgttgcgc gcgctgaagc
     4441 tcggacgcgg cgagcgcgtg ctgatcctca tgcgcgacac gctcgaagca gcggccgcga
     4501 tcctcggcac gattcacgcg ggcggcgttg ccgtcccagt ctccgagctc gcaacgcccg
     4561 acgacgtgca ggactacgtg ctccacgccg gcgcgggcat cgcagtcacc gacggcgcgc
     4621 acgaagcggt gctcgacgcg attcgcaacg agacgccgga ccttcgcgag atcgtctgcg
     4681 tcgatgcgaa gcttccgggc agccacgact tccagaaaat cgtcgatgcc gccgagccgc
     4741 aagccgcagc gccgagtggc cccgaagatg tctgcttgct cctctactcc gcgggctccg
     4801 gtcccggcga gctgcgcgcg gtgccgcact gccagcgcac gatcaaagcc gcgcacgaat
     4861 cattcgcgac gcagctgctc gagctcacct cgaaggatcg catcctgtgc gtcgcgcgcc
     4921 tctcgacggc gtacggcctc ggctccggtc tcttcctccc gctcgcggca caagccgaag
     4981 cgcttctctt ccccgagcaa ccgagcaccg cgcctctcct gcaagcgatc gacgcgttcc
     5041 agccgaccgt cctgttcgcg acgccgtcgg tctacgcgca gatggcgcac gacgccgaaa
     5101 aagaagcgat cgcaaagccg ctcggctctc tccgccacgc tgtcgcgggc gccgaaggca
     5161 tgcccgagcg cctcatcccg cgcattcgcg ccgtgctcgg cacggaagtc gtcgtcggct
     5221 acggcctgac cgaaatcttt cagttcgcgc tcgccggtcg cagcgacgat ccaggcgctc
     5281 gccccggcgt gtgcggcaag ccgctcgcag gcgtccaagt ccgcctcgtc gacgaagaca
     5341 acgacaccgt cggccccgac gaaatcggca cgctcgagct gcagtgcggc tcgctgttca
     5401 ccggctactg gagcggcggc ggccagagcg gcaacatgga gctgcgcgcc gacggttggt
     5461 tcacgacgcg cgatcgcttc atgatcgatc gcgagggctt ctaccaccac tgcggccgcg
     5521 tcgacgatct gttcaaggtc ggcggcaagt gggtctcgcc gtccgaagtc gagcgcgcgc
     5581 tcaccgccca cgaaggcgta tgggagtgcg cggtgatcgg cgccgacgac gacgaaggcc
     5641 tcatcaagcc gctcgcattc gtcgtcacga acgtcggctg ggaaggcggc cccaagctcg
     5701 aaggcgagct gaaggaatac gtcaagaagt cgctcgcgcc gtacaagtac ccgcgctgga
     5761 tcgagttcgt cgaggcgctg ccgcgcggcc ccggcggcaa gctgctccgc tacaagctgc
     5821 gcccgcagcg ccgtcgtcgt cgcgccgaaa ccggtagcca cgagctcccg tgacgctgag
     5881 ggtcgagacg ccgagcgtcg agatcgaccc cgctgttcgc gcacgcctcg gcgtcgagcc
     5941 gatcctcgtc gagcgaaagc tcggtcgcgt gcacccctcg tggctcgaag cgctcgatcc
     6001 gcggatgcgg gccgcgctcg aatcggtgtc gcgcgcagcg ctcgaatcac gcacgccatc
     6061 ggcggccgat gtgtggctcg ctcgctgggc cctcgccgat ctgcccgtcg acgcgcctgc
     6121 catcacgccg ctcgatctcg cgtggctcgt ccgcatcggc accgagcagc tctcgctcgc
     6181 gctcggccga tcgacacgca cggacatggg gcctcgccgc gcggcggtcg agcgatgcaa
     6241 ggacgcgaag cccgacgacg agctgtcgct ggtgcgcatc gccgcgcgcg tcctcgcgcc
     6301 gcatctcgcc gcgcaccgca acgcgcggct gcagctcacg cgtgcgcagc ctcgaccgat
     6361 cgggctcgtg atcgagcgcg agctcgtcgc gcacgccggc acgcctatcg atcacgttcc
     6421 gccgttaagt gtcctgcgct cactgtagct tccgaacagc tcggcttggc cccggatgga
     6481 acgcaagggg gctaggccgg agagggacgc aagaagaaac gggtagaggg tcgcgttctg
     6541 ctcgaacgat cctgcgtgca gagcgcgctc cccgaacagt ctccggcttg cgtcccttgc
     6601 tcttcttgct cccttgcgtt ccctccggga acgaacgggc gttccgggaa cgaacgggcc
     6661 ttgggctttt cgaagcgaac gccgacgcgc cgcgtctcgg gggcggcttc gccgacgacc
     6721 gagtccgcga cagcgtgtta ccgtcaacga cgatgaagcc ggggcgggtc atcgatggcg
     6781 acgagcacga cgcgcggacg ctcggccagc agctcgttaa cgacgcgcgg cgcgaagcgc
     6841 aggaccttct cgatcgtgcg cgcgcggatg ccgagaagat cggcgcgcag gccgcggccg
     6901 atgcagcgac cgtgcggcag cgtgcgggcg tcgttacgtc gagcgagacg ccgagcggtc
     6961 gcgttgatgc ggtgacgggc ctcgtcgttc gcgcgacgct gtcggccgtt tcgctcggcg
     7021 agatcgtgat gatcgatcgg ccggggctgt cgctgagggc ggaggtcgtc ggattttcgg
     7081 gggagcaggc ggtgctgttg ccgctcggcg atttggcggg catcgcgccg gccagcccgg
     7141 tgtggcggac gggggagccg ctgtcgattc gcgtcggcga cgaattgctc gggcgcgtgc
     7201 tcgacggggt agggcagccg atggacggcg gcacggcgat cacgagcggc gaagcgtggg
     7261 cggtcgaccg gccggcgccc aatgcgttgt cgcgaccgcc gatcagtgcg cacatcacga
     7321 cgggcgttcg cgcgatcgat gcgctgctga ccttggggcg agggcagcgt gtcggattgt
     7381 tcgcggcggc gggcgttggc aagtcgacgc tgctcgcgca gatcgcgcgt gggacggatg
     7441 cggatgtgat cgtgctgtgt ctggtgggag agcgcgggcg agagctcgcg gagctgctcg
     7501 ataacgagct cgcgccggcg cgtgcgaaga cggttgtcgt gtgcgcgacg agcgatgcgc
     7561 cgccacttgt aaggctgcgt gccgtgcata cagccacggc gatcgcggag tggtttcgcg
     7621 atcggcgagg cgcgaacgtg ctgctgctcg tcgattcgtt gacgagagtg gcgagggcgc
     7681 agcgcgaggt cgggctgtcg gcgggtgagc ccgtcgcgag gcatggttat ccgccgagcg
     7741 tgtttgcgct gctgccgcga ctgatcgagc ggacgggcgc gacgcgcgag ggcgcgatca
     7801 cggcgctcta taccgtgctc gttgcgggca atgacatgga cgagccgatc gcggatgaag
     7861 tgcgcggcct cgtcgatggc cacatcgtgc tcgatcgccg gctcgcgcag cgcgggcatt
     7921 ttccgccgat cgatgtcgtg gcgagcgtgt cgcgtctgat gccgcggctc gttgcggcag
     7981 atcatgcgaa ggctgcagcg cgcgtgcgcg agcgcttggc gatctatgaa gagcatcgcg
     8041 acctcatcac gctcggcgcc tatcagagcg ggagggatcg caaggtggac gacgcggtca
     8101 gcgcataccc gcgcatcgaa gcgctgttgc ggcagtcgag ggatgaaacg gccgagtggg
     8161 acggttcact ttcccggctc ctcactatca atggctgacg cccgggggcc ccggggatgg
     8221 ggacgattgc cccgatagat aggtcgacgc cgatatgctg gcgcatcacg cggtgtgacc
     8281 aatgcgaggt gccatacaat gatgaacgag cctccgctga gttgacgggg acttccgctc
     8341 tagccgatcg ctacccgatg atggcgcacc ccttgctgac acgagcctcg ccatccctat
     8401 gcagcaacgg cggcgttcat ccaagatcac gaaccccatg acccctatcg gtccggtgcc
     8461 gtttgtcggt cggcagagcg agctcgagga gatcaagcag caccttgcgg tggtgccact
     8521 ggtctctctg catggcgcgc tcgggtccgg caagacgcgg ctcgtgacgg agctcgcgcc
     8581 gatgctcggt gcgcagacca cgatcatcga gtgccacccg ggcgaccggg ccagcgcact
     8641 gcgggctcgt gcagagcgcg cacttcgctg cgttcccggc acgctcgcgc agacgctcac
     8701 cgcgcaagcg cgcgtgctcg tcatcgatga catccagcat cttcggatcg acgaggcgac
     8761 gaacctcctg tcgccgctgc tcctcggcca gaccgcgctc ggccgcgtgg tcgtcgtcgg
     8821 ccgcgatcca ttgtccgccg ccgtcggcgc atcgatcgcc gagatcgagc tgcacggcct
     8881 cgacgtcgac tcggcgtcgg cgctgtgggc atcgctcgaa gaacagtacg gcgcgctcga
     8941 agccgaaggc acgttcgata ccgcgtacgg ccgcacgcgt ggtgtgccgc tcgcgctccg
     9001 tcgcgaatac gcgcgcgccc gcttcggcgc gaatgcgtgg gatgtcccgt cgctcgacga
     9061 gccgtcgcgc atcgcgatcg aagcgctcgc cgtccttcgg caacccgtcg cgccggccgc
     9121 cgtttcggcg ctcgcgccag gcgtcgatac cgaagctgcg ctgacgagcc tcgtcgcgcg
     9181 ccagctcatc gatgccgtcg gcgatggccg cctcgtcgtg cacgaggtcg tgcgcgtcga
     9241 cgtgctctcg cagatggctc ccgaggagcg caagcgcctg tccgcatctg ccgcggagct
     9301 cgtcgcgtcg accgccgcgc tctccaaggg gccgcgcctc gcgtggcaag ccggcgacga
     9361 cggtgcattc ggcgcgatgg atcaagtcac gcgcgtgcgc gaggtcgtgt ggcactggct
     9421 cgccgccggt caacgcgaaa aggccgccga cgttctgctc gcgcatcgcg agctcgccgc
     9481 tcgctccggt ggcgcaggcg agttcgaagc gctgctcgaa gccgtcagcg atcgtccgcg
     9541 ccgccgcgcc ggcacgcagt ccgacgttgt gcccgagatg ccgctcgacg agaagcgctc
     9601 gcgcgcgctc gccgctctcc gcatcgagat cgccgttcgc ggcggccgct tcaccgaagc
     9661 gttcgagcgc tcgaccgccg cgccgggcgc cgtctcgcag ctcattcaag ccgagctcac
     9721 gctcgcatcg ggcgacgcca gtggtgcgcg ccgcgcactg caaggcctca tcggctccga
     9781 cgatccgagc gagcgtgcac gcgcgatcgc gatgctcgcc gagctcgagc tcctcgccgg
     9841 aaagcccgac gccgcagccg cccacctcgg ctcgctcggc gatgtcgcgc aagccgatcc
     9901 actcgctcgc gcccgcgttc atctcgctgc cgcgcgcgcc gacgaatacg cgggccgcgt
     9961 cgctgccatg cgctcagcgc tcgctcgcgc tcacggcgca tgcaagagcg ttcccgcgtg
    10021 cctcgaagcg agcgagctca cggccgtcgt cgacgcacgc cgcgcgatcg gtctcgcgcg
    10081 cgaaggtcgt ctcgtcgaag cagccaccgc gctcgacgcc gccgacgccg ccgctcgcga
    10141 tctcgacgcc gtcgcggtcg ccgacgaagt gctcgccgct cgcgcgctcg tggcgcggcg
    10201 ccgcggtgac agcgccggcg ccgccgcact cctccacgat ctcgtccgcg cgcgccgtga
    10261 acgtggcgac gagctaggtg ccctgcgcgc cgagctcgat ctcgcagacc tcgaaatcct
    10321 gcgcggccaa cccgctctcg ccgccgagct cgcaagcgcg gctctcgcat cgagcgttcg
    10381 tcgcgagctc ggtcacctcg cggcacgcgg gtccgccgtc gtcgctgcga tcgatctgct
    10441 cgagcttcgc ctcgacgccg cactccctcg cctcgaagag ctctacggcc agcccgctct
    10501 cgacgccgca tcgagcgcga acgt
//
LOCUS       DATLBZ010000349         3790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5628366, whole genome shotgun sequence.
ACCESSION   DATLBZ010000349 DATLBZ010000000
VERSION     DATLBZ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5628366"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1230)
                     /locus_tag="VMZ53_18010"
     CDS             complement(130..1230)
                     /locus_tag="VMZ53_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS30412.1"
                     /translation="MDVLAWQREVGQAWTNVAADLLGISLPSSPPLAQTPRDEFPLEG
                     GARLYRFRAAAPSQALPARATRPFLLIPSMINRWYVLDLRPGASLVEALVRAHFDVWC
                     LDWGTPEAEDRYLDWEAVLARLGRAVRRVKRETGADKLGVLGYCMGGTLTTIYAAQHA
                     DEIAALVTLAAPIDFQNGGQLRCMVEPQWFDADAIADAGNVEPTQMQSGFTALRPTLE
                     LGKLASLPDLMSDAKAREGFLALDAWASDNIPFPAEAYRRYIREMYQANQLVDGSHRV
                     ANRDVKLSAITCPTLVITASRDQICPPAAATALLDLIGSTDKQVLDVPGGHVGAVVGS
                     RAARDMYPALVRWLEARLAATVQPPSTPPSRL"
     gene            complement(1240..1611)
                     /locus_tag="VMZ53_18015"
     CDS             complement(1240..1611)
                     /locus_tag="VMZ53_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30413.1"
                     /translation="MSQEKTTKTETPKSETSGAAQQQNPFASFMPFAPFGAFAAADPM
                     KMWEQTHQAWQKIFSDAQGQAQKWADEYASMEKQLFTRANQAVDTWAQLAKDSIAYTE
                     QLSSQARKLGFDAARKAGFGA"
     gene            1754..3112
                     /locus_tag="VMZ53_18020"
     CDS             1754..3112
                     /locus_tag="VMZ53_18020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS30414.1"
                     /translation="MTEQKPRWAAFWAAWVGWVLDAFDFTIYIVVAQKLIQEFGVSPT
                     AIGGSLTLTLLVRLAGGFCAGWMADRWGRRLPLMMSLVWFAAFDAAIYFAPSFTWIII
                     FRTLFGFGMGAEWTAGTSLAMESFPAKSRKLASGLLQAGWPVGFLLAAAVAHFVVPTY
                     GWRPMFLIAAVPALLVIPIRLFVPNDHAAPDEQLQTSKGALKDLLEPRVFKMLVLGSL
                     VMALGFIVYYGMVTSHMGIHFEVANCGKDAKCIAATYSHGWNNQMIFNVGMLVGVVLA
                     GWIASRWSVIAALVVPALLMVPALPLFVGSAGNVMWLGAFLGGMLGVGYSGVTPVLTT
                     SLFPAHVRARAIGIVYHVGALLAAFIPTMIPWVVKTAHISLATTVTIVVGAGLVVMSA
                     AVLVLRGYLTTPAVETRLEPSPVAAQPPVTPGHPREMHPVAVAIVDHEVARAAERRAL
                     LG"
     gene            3229..>3790
                     /locus_tag="VMZ53_18025"
     CDS             3229..>3790
                     /locus_tag="VMZ53_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30415.1"
                     /translation="MVAASATAGATLPPESSEEITDLARLRTDGTPTGNPGTIQLVAA
                     LETRARRASAPLDRDAPRYELGAPIGRGGMGEVIAAHDLQIGRHVAIKRLHAATPTDV
                     QLARFLREARIQGRLEHPSIPPVYELAHDDDGRPYFAMRKLEGVTLSETLRNASSPFT
                     RQRLLRAFVEICHAIDLAHSQRIIHRD"
BASE COUNT          556 a         1266 c         1253 g          715 t
ORIGIN      
        1 ctgcggcatg aaagagcagt gcgggtggca gagcgatggc tgttacgact acgtgtggtg
       61 cgggccgtgt ccaggcgaca aaaggccgag cgcaccaccc gcgaccgacg gcatctacgt
      121 catcgcgctc tagagccgcg atggtggcgt cgacggcggt tgcaccgtcg cagcgagccg
      181 agcctcgagc cagcggacga gcgcgggata catgtcacgc gctgcgcgac tgccgacgac
      241 ggcaccgacg tggccgccgg gaacgtcgag cacttgcttg tccgtcgagc cgatgagatc
      301 gaggagcgcg gtcgcggcgg ctggcgggca gatctgatcg cggctcgccg tgatgacgag
      361 cgtcgggcat gtgatcgcgc tcagcttgac gtcgcggtta gcgacgcgat gcgagccgtc
      421 gacgagctga ttcgcttggt acatctcgcg gatgtagcgg cgatacgcct cggccggaaa
      481 cgggatgttg tcgcttgccc acgcgtcgag ggcgaggaag ccttcgcgcg cctttgcgtc
      541 gctcatgaga tcgggcaacg acgcgagctt gccgagctcg agcgtggggc gcagcgcggt
      601 gaagcccgat tgcatctgcg tcggctcgac gttgcctgcg tcggcgatcg cgtcggcgtc
      661 gaaccattgc ggctcgacca tgcagcgaag ctggccgccg ttttgaaagt cgatcggtgc
      721 tgcgagcgtc acgagcgcgg cgatttcgtc ggcgtgttgc gccgcgtaga tcgtcgtgag
      781 cgtgccgccc atgcagtagc cgaggacgcc cagcttgtct gcaccggtct cgcgcttcac
      841 gcgccgcacc gctcgcccga gccgcgcgag caccgcttcc caatcgaggt accggtcttc
      901 cgcttcgggt gtgccccaat cgaggcacca cacatcgaag tgggcgcgca ccagcgcctc
      961 gacgaggctc gcgcccggcc gaaggtcgag cacgtaccag cggttgatca tcgatgggat
     1021 gagaaggaac ggccttgttg cccgcgcggg gagcgcctgt gacggcgcgg cggcacggaa
     1081 gcgataaagg cgcgctcctc cttcgagcgg aaactcgtcg cgcggcgtct gggcgagcgg
     1141 cggagaggac ggcagcgaaa tgccgagcag gtctgcggcc acgttggtcc acgcctgtcc
     1201 gacttctcgc tgccaagcga gtacatccac cgtcattcgt tacgctccga agccggcttt
     1261 gcgggcggcg tcgaagccga gcttgcgagc ctgcgacgac agctgctcgg tgtacgcgat
     1321 cgagtccttc gcgagctgcg cccacgtgtc gacggcctgg ttcgcgcgcg tgaacagctg
     1381 cttctccatc gacgcgtact cgtcggccca cttctgcgcc tggccttgcg cgtcgctgaa
     1441 gatcttctgc cacgcctgat gcgtctgctc ccacatcttc atcgggtcgg cagctgcgaa
     1501 cgcaccgaac ggcgcgaacg gcatgaacga ggcgaacggg ttctgctgct gtgcagcacc
     1561 ggaggtctcg gacttcgggg tctcggtctt ggtggtcttc tcttgcgaca tggaatagct
     1621 cctttgtgcg aatcacccta agggcgggat ccggagctgt caacccgttt tgctgcatcg
     1681 caacaatttt tcttcctcgg gaccgcgttt tctgtgttgc aacgcaactc aaagatcggc
     1741 tactcaacct cgcatgacgg agcagaagcc tcgttgggca gcgttttggg cggcgtgggt
     1801 cggctgggtt ctcgatgcgt ttgacttcac gatctacatc gtcgtcgcgc agaagctgat
     1861 ccaggaattc ggcgtctcgc cgacggcgat cggtggctcg ctcacgctga cgctgctcgt
     1921 gcgtctcgcc ggcggctttt gcgcggggtg gatggcggat cgctggggcc gccgcttgcc
     1981 actcatgatg tcgctcgtct ggttcgcggc gttcgacgcg gcgatctact tcgcaccgag
     2041 cttcacgtgg atcatcatct tccgcacgct gttcggcttc ggcatgggcg ccgagtggac
     2101 ggccggcacg tcgctcgcga tggagagctt cccggcgaag tcgcgcaagc tcgcctcggg
     2161 tctgttgcag gcgggatggc ccgtcggctt cctgctcgcc gccgcggtcg cgcacttcgt
     2221 ggtgcccacc tacggctggc gtccgatgtt cctcatcgcc gcggttcccg cgctacttgt
     2281 aattcccatc cgcttgttcg tcccgaacga tcacgcagca cccgacgagc agctgcagac
     2341 gtcgaagggc gcgctgaagg atctcctcga accacgcgtg ttcaagatgc tcgtgctcgg
     2401 ctcgctcgtg atggctctcg gcttcatcgt ctactacggc atggtgacgt cgcacatggg
     2461 cattcacttc gaggtcgcca actgcggcaa ggatgcgaag tgcatcgccg ctacgtacag
     2521 ccacggctgg aacaaccaga tgatcttcaa cgtcggcatg ctcgtcggcg tggtgctcgc
     2581 aggttggatt gcgagccggt ggagcgtcat cgcggcgctc gtcgtccctg ctctgttgat
     2641 ggtgcccgcg ctgccgctgt tcgtcggttc ggccggcaac gtgatgtggc tcggcgcgtt
     2701 cctcggcggc atgctcggcg ttggctactc cggtgtcacg ccggtgctga cgacgagcct
     2761 gttcccggcg cacgtgcgcg ctcgcgcgat cggcatcgtc tatcacgtcg gtgcactgct
     2821 cgcggcgttc attccgacga tgatcccgtg ggtcgtcaag actgcgcata tctcccttgc
     2881 gacgaccgtg acgatcgtcg tcggtgccgg cctcgtcgtg atgtctgccg cggtcctcgt
     2941 gctgcgcggc tacctgacga cgccggccgt ggagactcgc ctcgagccgt cgccggtcgc
     3001 cgcccagccg cccgtcacgc ccggccatcc tcgcgagatg caccccgtgg ccgtcgcgat
     3061 cgtcgatcac gaggtcgccc gcgcggcaga gcgtcgcgcc ctcctcggct agacgcgacc
     3121 gactaccgcg gcgttcatcg tcggaaccat cgtccccagt gtcgcggacg taatcccgcg
     3181 ttcgacataa cggcacgcgg cggcatcacg gttgcacttc ccgtccgcgt ggtggctgcc
     3241 tctgcgaccg cgggcgcgac gcttccgccc gagagctccg aggagatcac ggatctcgcg
     3301 cggcttcgca ccgacggcac gccgaccggt aatcccggca cgatccaact cgtggctgcg
     3361 ctcgagacgc gcgcgcgccg ggcatctgct ccgctcgatc gcgatgctcc ccgctacgag
     3421 ctcggcgcgc ccatcggccg cggcggtatg ggcgaggtca tcgccgcgca tgatctgcag
     3481 attggccgtc acgtcgcgat caagcgcctc cacgcggcga cgccgaccga cgtccagctt
     3541 gcccgcttcc tgcgcgaagc ccgcatccaa gggcggctgg agcatccgtc gatccctccg
     3601 gtgtacgagc tcgcgcacga cgacgatggc cgtccctact tcgcgatgcg caagctcgaa
     3661 ggcgtcacgc tatccgagac actgcgcaat gcgagctcgc cgttcacccg gcagcggctg
     3721 cttcgcgcgt tcgtcgagat ctgtcacgcg atcgatctgg cgcattcgca gcgaatcatt
     3781 caccgcgatc
//
LOCUS       DATLBZ010000350        14493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9351421, whole genome shotgun sequence.
ACCESSION   DATLBZ010000350 DATLBZ010000000
VERSION     DATLBZ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14493
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9351421"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VMZ53_18030"
     CDS             complement(<1..531)
                     /locus_tag="VMZ53_18030"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HUS30416.1"
                     /translation="MIRTKVLGLGSYVPDRVVTNDEITYLNDKHVRQEQPVTETNDQW
                     IQQRTGIKERRYVANDGSQWTSDLAVEAAKRAIADAGLQPSDIDCIILGTLSPDFHFP
                     GTGVIVQKKLGIERGNCPCFDIRQQCSAFVYSLQMADAFIRTGMYKRILMIGAELHSH
                     SLDYSTRGRDVMVLFGD"
     gene            complement(580..1416)
                     /locus_tag="VMZ53_18035"
     CDS             complement(580..1416)
                     /locus_tag="VMZ53_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30417.1"
                     /translation="MFRATQRIVRTARAAALPIATFVVSACATGAQTQTQSQVDASTS
                     SGEHDAAVVAMPDAHGVVTPDAATVASCGISEGFTPALDGVDDMAAYPAAQQLAPGAS
                     LGSDAAAIAWDATNVYATVTSSAFANPYEPLHIYLEAATAFAAATPASGKEYSGLVAQ
                     LPFAPTHLIAVRRVSDSGTGAYDGVFVPSDGWMTRTVGLDSATLVSVDQKTVSVTVPW
                     TALGNCPTKLRLAVHVVHGASANEWKDLVPSTHTPWQSPGGGYYEIDLTGAPAVTSWS
                     LR"
     gene            1633..3249
                     /locus_tag="VMZ53_18040"
     CDS             1633..3249
                     /locus_tag="VMZ53_18040"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HUS30418.1"
                     /translation="MRVCFVVALLLAACRTTGTGRGAGGSAGGAATGGAGVAPAGHSA
                     RLVVVLVIDQFPEWSLEQKTSQLTAGGFARLFAEGEWHVGHHPSAATLTAPGHALLGT
                     GKPPAYSGILGNEWWSRDEDRVVHAVESDDPMKFLRVPGLGDAVARGRSGAKAVAVSL
                     KERAARLPLGAHGLAVWYEPKTTTWNANGDSLGEHPATAAASAQQLPAWIGKLPAIAP
                     RIATWSAGDPAKLATLSGTVDAQPGENGEKGFGPTFPHDPKATKDPSDAVYAMPLGNE
                     LVLETALAAIDGEGLGRDDKPDLLFVSLSANDYIAHGWGHESWESWDSLLRLDEKLAT
                     FLDGLDQRVGARNWALVATSDHGGAPMPERLDGGRLTFEQLLEAANNAASVELGPGSW
                     VASAHYPFVYLSKAALAQPKRSLAIVIKKIEYALRSFPGIARAGRVDTYAGNCEQRTG
                     DEQALCLTFDPQRSGDVFYIPARGWILEGADDHYATAHGSLNDYDRDVPVLLLPFGRT
                     PHTPDAKPRDQIGLEEIAPLVSAWLGVAPP"
     gene            3265..4176
                     /locus_tag="VMZ53_18045"
     CDS             3265..4176
                     /locus_tag="VMZ53_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30419.1"
                     /translation="MRWLWLLLAGCGRLSFDALGDGDASSGIDTAAPDACSFSAFAPP
                     TRLPGQVQSAVDDWAPRSALGKTQIFFCTYNGSAGLCDIVYATRPDNTSPLSAPMFIP
                     ELDSAAYEGGPSVTEDALDMVFFRDGAAYDLFETSRASISDPWQPPQPLAINSTMSDM
                     SPSLSPDGLRMVFASARAGSLGGGDIWETTRTSRTAAWDPPVHRIELSSASDEGGPTM
                     SPDGLEVFFASNRAGGLGLIDVYTARRLALGNPFGAPTLVPELSSTRDDISLSMSSDG
                     RSLFYNYDSVSAGGQNADLWVATRTCN"
     gene            complement(4173..5684)
                     /locus_tag="VMZ53_18050"
     CDS             complement(4173..5684)
                     /locus_tag="VMZ53_18050"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HUS30420.1"
                     /translation="MVASLETDPTIGALLGETTTEIDVARKNRVFCNRNLRMDSIEMI
                     GFDMDYTLALYHQDKLEQLSIALTLQKLVEKHGYPEEIRSLSYDPRWAIRGLMVDRLN
                     GNVFKMDRHAYVGRCYHGFRELDHDQRRATYRNEKINLSADRFEWIDTLFGLPEAVMY
                     TTLVDWADRQTGTVDYEKMFNDIRTAIDEAHRDDTLKSVIKADIPAFIMKDPLLGETL
                     HKFRSSGKKLFLLTNSLYDYTSVIMSYLLDGERKAYPSWRNYFDIVIVGGAKPAFFQE
                     LRPFVQIDPNTMQPVATNGEIKHLSRDKIYQGGNVIAFEQMTGIKGEQVLYIGDHIYG
                     DILRLRKQHMWRTAMVLQELEREISVIERLDPQIRDLDLLDRRHRNLESEIDYQSLRL
                     KKIQRLLDDDSCSAALRARLEEERKLARASVDTLRDRAALMEQEVDSLEARIDRAYNS
                     HWGSCLREGNENSRFGEQVNDYADLYTSRVSNFGPYSPLRYFKAPRRPMPHEI"
     gene            5724..6290
                     /gene="hpt"
                     /locus_tag="VMZ53_18055"
     CDS             5724..6290
                     /gene="hpt"
                     /locus_tag="VMZ53_18055"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HUS30421.1"
                     /translation="MASPPPAWNRPDDPFAELISAEAIAARVAELGAQITTDYRPLIG
                     QAEVVVIGVLKGSIIFLADLVRHIALPIACDFIGISSYGDATVSSGVVRITQDLSKSI
                     EGKHVIVVEDIVDTGHTVHYLLENLATRRPATIKLASLLHKPERQERQVKIDYLGFTI
                     PNKFVVGYGLDVAQHYRNLPFIGFVQNA"
     gene            6287..7285
                     /locus_tag="VMZ53_18060"
     CDS             6287..7285
                     /locus_tag="VMZ53_18060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HUS30422.1"
                     /translation="MKPDALAKLPAVLFEIGKLIGSDLDPGILLSRIAELLCQLVNAK
                     ACSVMLLDANRKRLLAKAAYGLRTERMHTLSFAVGEGVAGWVVERGESALIGDVSKDA
                     RFVALPANQTPIASMLCVPLLSRGERVGVVTATAEHTDAFDTEDLEIVRFIATTIALD
                     IENVRLHRVAVTDPLTGTFNREFLQQRLPAEIEATIERDRPLSIALVDVDHFKAVNDQ
                     YGHGVGDVVLAEVARRLRSAIRGGDLLVRYGGEEFIAVLPKADAGRAWEVGERMRQRV
                     CERAFDVGDGLALLLRISVGIAQWRMGEKMPDLIERADVALYGAKQRGRNRVEVAP"
     gene            7282..8514
                     /locus_tag="VMZ53_18065"
     CDS             7282..8514
                     /locus_tag="VMZ53_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30423.1"
                     /translation="MSSSGDPPKKDPPSTAPAAPADDREIGSVDELYGFAPDPFMDSY
                     EEPAPPAAPTAEPIAVTEPVIATVTAEPLAASPTDVAPAAVEPVDEPLLPTTTDDNAL
                     REAVGIPTPKKRTTAKMDAVGDDEPKDKKVKYVDPDASLWSRKLLTLIAVGVLAIISI
                     AVLVILGSVNSRRYEFTCSSDRVGAEQGRSFPPWGTEQMTGPEWKPIALPPNAECTPR
                     DTENKSELEGWYLQALVDRATTTLTTPNLVEQITAAPAGKGGNALDIVAAQLQQALLL
                     SRSADHRDQRKEVERLMGDVQYWRASLRLRDASAALLDASKQFETAAQQRPRHVTDAA
                     AWSQYVKKLADDLRAGPNGAPAVQTFPPTPTATPPTGDRPSAPAGVALPVETPPSQHD
                     ETPPPTPDAGVPTGGVLL"
     gene            8524..9135
                     /locus_tag="VMZ53_18070"
     CDS             8524..9135
                     /locus_tag="VMZ53_18070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HUS30424.1"
                     /translation="MHRLLSEEVMTRNVAMIVGSLRKDSYTRMVANALAELAPKSLHL
                     DHAEIGNLPLYNQDLDAENRAPAEWTQFRDRMRDDAAVLFLTPEYNRSVPGVLKNAID
                     VGSRPYGQSIWSGKPAAIVSCSPGMYGGFGANHHLRQSLVFLDMPCLQQPEMYLSRVD
                     KAFKDGKLVDDTVRELLTGFITKFENWVDKYVTYVAPSQKKSA"
     gene            9157..10161
                     /locus_tag="VMZ53_18075"
     CDS             9157..10161
                     /locus_tag="VMZ53_18075"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HUS30425.1"
                     /translation="MRAPISLILVALFGCREGKETKPVIADHHTAPPVTAPVPPTATP
                     DPWAAGPADSADDPPSLAERHKRAEEACPLVKAPYFYSIEKAGKVSHILGTRHISVPL
                     AKFPKEVTDAISTAKLAVFEVAPDDNSGVETPKISIKNELGPDLWKKYEALVGKAAAE
                     SLEEATPAEAMIWLIAMYEDIGAMLDSEIQRQVQAAKIPTMGLETSAFQDTLLQKLLD
                     IRMLKAEVAHTDGRDELAKESKEDLSEYCAGSDDKPGMDDDTRKDLLEAGYTAAEIDS
                     IDDQMVYQRNASWIPKLEKLFAKGDVFVAVGADHLTGDRGVVALLAKRGFTVKRVPRK
                     "
     gene            complement(10182..10883)
                     /locus_tag="VMZ53_18080"
     CDS             complement(10182..10883)
                     /locus_tag="VMZ53_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30426.1"
                     /translation="MMRFIVLVLIAVFVVLVVIAGIVSPRTARAATIATDPAPAAASD
                     STGVIVDAPHDVVLGRFLYEPYIVRGGDEPHLGGRFGVDMIGANGEGATKIAGGIRFT
                     LGGDGAGAFGTEGQLLIGVVRTISDRAAVALVAPLGFTLGGGTDAAMSGSNFGAHGYA
                     GVEGIAAIGRFASEDGPGVRGIELAAGISNRGARARAGIVRPGKDVGWGVGIDWQRDG
                     AFELIGVYFATTAGR"
     gene            11147..12370
                     /locus_tag="VMZ53_18085"
     CDS             11147..12370
                     /locus_tag="VMZ53_18085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS30427.1"
                     /translation="MNRESWSIPPLRDYQRAAVQAWRDAGDRGVIVLPTGAGKTIVAL
                     AAIAELAVSTLVLVPTRILLDQWARFFGMCWAHPIGRLGDGQRSIAPITIATYASAIT
                     WAPRIGDSFGLVVVDEAHHVGAWCPREILEMLVAPSRLGLTATPPDGVAASALARHVG
                     PTVFECGIADLTGTALAPYTHETIAIELTAEERSAYRSTRGLFAAYYARARRDDPNMH
                     WRQFVHEAQKSDDGREAVLAWRASRTIVSYPEAKRSKVRELLARHAGDRVLVFTADNA
                     TAYAISRELLVPPITCDIRRKERTEMLSRFSSGACNVLVSAQVLDEGFDIPDADVAIV
                     VGGTSSQRRQVQRIGRVLRPRPGKHAVVYELVVEGTAEVRQSFDRATNRSHRGARRHP
                     EIVRQDALSSQEVWS"
     gene            12367..13590
                     /locus_tag="VMZ53_18090"
     CDS             12367..13590
                     /locus_tag="VMZ53_18090"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HUS30428.1"
                     /translation="MKLLTETDLSWIADMLDVVVACQGQPWRTALERLEGLAPELESS
                     SSQRVSQKRVAAVLGAIQRVLGRSTKADLARTCRALVLGRPALEPAERLDRIAAAARS
                     LDISVATVEQLLWIDLPRERPVELPFGRPDELEIAAFANMQLIEKTLRRAQTAQLRIR
                     GDAGPFLRGAARCGLLTTARPEPVAPPVPVAPPEDRTTTREDIDHLHGAIEQAKPVRV
                     EPVRVEPVTLIDIVGPLALFHRTAVYGRALSALVPLLAEVSAFELVVMSTGFKGEYAV
                     ELGSPALLPRTSAALHGPSKQAARLARELRELLPDRRITIAPPAVVADGAYLCPDLLV
                     GETYVELVGFWTAEHLARKRGAYEAAGLRVALCADELRGCAGEELPPDVIGYTKYVDA
                     DVVARRVDARRIVPR"
     gene            complement(13587..14081)
                     /locus_tag="VMZ53_18095"
     CDS             complement(13587..14081)
                     /locus_tag="VMZ53_18095"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HUS30429.1"
                     /translation="MALGKLPDQDADEDGGSDGIFAEINITPLTDVFLVLVIIFMVSA
                     LAVQVEARQEKKKVAEAQEQSEIEKKSGLKVNLPQGQAQEIDTTKQSLVITVPLQGDV
                     DVGGKRIPDADLDNLFRAAFARDKNTQVVLKADKGVQHGRVVNLMERAKQAGLTRLAI
                     GTSG"
     gene            complement(14059..>14493)
                     /locus_tag="VMZ53_18100"
     CDS             complement(14059..>14493)
                     /locus_tag="VMZ53_18100"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HUS30430.1"
                     /translation="VHRERMRVNQDLRSRLWMLGTIGATAPFVGLFGTVLGIMSAMGG
                     FKGDDEVKFSMVSGPISEALVVTAAGILVAVEAVILFNFFSQRASRIATEMKLLTDEF
                     LELLLDQPRDQQPKGKRVDEAKADSDTKARTKGDGDGSREAA"
BASE COUNT         2440 a         4883 c         4668 g         2502 t
ORIGIN      
        1 atcgccgaag agcaccatca cgtcgcggcc ccgcgtcgaa taatcgagcg agtggctatg
       61 cagctccgcg ccgatcatga ggatgcgctt gtacatgccg gtgcggatga acgcgtcggc
      121 catctgcagg ctgtacacga aggccgagca ctgctggcga atgtcgaagc aaggacagtt
      181 gccgcgctcg atgccgagct tcttctgaac gatgacgccc gtgccgggaa agtgaaagtc
      241 aggcgagagc gtgccgagga tgatgcagtc gatgtcactc ggctgaagcc ccgcgtccgc
      301 gatcgcgcgc ttggccgctt cgacggcgag gtcgctcgtc cactggctgc cgtcgttcgc
      361 gacgtagcgg cgctccttga tgccagtgcg ctgttggatc cactgatcgt tggtctcggt
      421 gaccggctgc tcctgacgaa catgcttgtc gttcaggtag gtgatctcgt cgttcgtgac
      481 gacgcgatcg gggacgtaac taccgagccc caggaccttc gtgcggatca ttcaggggac
      541 atagcacaca gccgtgtcag gtttcggtca caggactcgc taccgaagtg accagctcgt
      601 aacagcggga gcgccggtca aatcgatctc gtagtagccg ccgcccggcg attgccacgg
      661 cgtatgcgtc gacggaacga ggtctttcca ctcgtttgcg ctcgcgccat gcacgacgtg
      721 cacggccaac cgcagcttcg tcgggcaatt cccgagagcg gtccacggca ccgtcacact
      781 cacggtcttc tgatctacag acacgagtgt tgcgctatcg agccccaccg tccgcgtcat
      841 ccagccatca ctcggcacaa agacgccgtc atacgcgccg gtgcccgaat cgctgacacg
      901 cctcaccgcg atcaagtgcg tcggcgcaaa tggcagctgc gcaacgagcc cgctgtactc
      961 cttgccgctc gcgggtgtcg ccgccgcaaa cgcggtcgcc gcttcgaggt agatgtgcag
     1021 cggctcgtac ggattcgcga acgcggacga cgtgaccgtc gcataaacgt tcgtcgcatc
     1081 ccacgcgatc gccgccgcat cgctgccgag cgaagcgccg ggcgcgagct gctgcgccgc
     1141 gggatacgcc gccatgtcat cgacgccatc gagcgccggc gtgaaacctt cgctgatgcc
     1201 acagctcgcg accgtggccg cgtccggtgt gacgacgcca tgcgcatccg gcatcgccac
     1261 caccgccgca tcatgctcac cgctgctcgt cgaggcgtcg acctggctct gcgtctgcgt
     1321 ctgcgcaccc gtcgcgcacg ccgacacgac gaacgttgca atcggcaacg ccgcggcgcg
     1381 ggctgtccgc acgattcgct gagttgcgcg aaacatgtcc ggcagccgag cactcgctgt
     1441 gccaccacgt cgcatttaag ggacggacgc aacttccgca acccgccgca accgccgaaa
     1501 gggacggagc ccctccgtcc ctttcgcaac ctgcgcaacc agaggttgcg gaagttgcgt
     1561 ccgtccctta cgggttgcgg aagttgcgtc cgtcccttag cggtgtgcaa gtcgcggtat
     1621 tgtgcgggcg tgatgcgcgt ctgctttgtc gtcgcgttgc tcctggccgc gtgccggaca
     1681 acggggacgg ggcgcggcgc gggcggcagc gcgggtggcg cggcaacggg cggagcgggg
     1741 gttgcgccag ctggtcacag cgcgcgactc gtggtcgtcc ttgtcatcga tcagtttcca
     1801 gagtggtcgc tcgagcagaa gacgtcgcag ctgacggccg gcgggtttgc gcggttgttc
     1861 gcggagggcg agtggcacgt cgggcatcat ccgtcggccg cgacgttgac ggcaccgggg
     1921 catgcgctgc tcggaacggg caagccgccg gcgtactccg ggattttggg caacgagtgg
     1981 tggagccgcg acgaggatcg cgtggtgcac gcggtggagt cggacgatcc gatgaagttc
     2041 ttgcgcgtgc ccgggctcgg cgacgcggtt gctcgggggc gaagcggtgc gaaggcagtg
     2101 gctgtcagct tgaaggagcg cgccgcgcga ctgccgctcg gggcgcatgg gctcgcggtt
     2161 tggtacgagc cgaagacgac gacgtggaat gcgaacggcg actcgctcgg tgagcatcct
     2221 gcgactgcgg ctgcgagtgc gcagcagctt ccggcgtgga tcggcaagct gcctgcaatt
     2281 gcgccgcgca ttgcgacgtg gtcggcaggc gatccggcga agctcgcgac gctctcggga
     2341 accgtcgacg cgcagcccgg cgagaacggc gagaagggct ttggcccgac gttcccgcac
     2401 gatccgaagg cgacgaagga tccgtcggac gcggtgtacg cgatgccgct cggtaacgag
     2461 ctcgtgctcg agactgcgct tgcggcgatc gacggcgagg gactcggtag agacgacaag
     2521 cccgatctgc tctttgtctc gctgtccgcg aacgactaca tcgcgcatgg ctgggggcac
     2581 gaatcgtggg aatcgtggga ctcgctgctg cgcctcgacg agaagctcgc gacgtttctc
     2641 gacggtctcg atcagagagt cggcgcgagg aactgggcac tcgtggcgac gagcgatcac
     2701 ggcggcgcgc cgatgccgga gcgcttggat ggtggccggc tgacgttcga gcagctactc
     2761 gaagctgcaa acaacgcggc gagcgtcgag ctcggccccg gcagctgggt tgcgagcgcg
     2821 cactacccgt tcgtctatct gtcgaaggcg gcactcgcgc agccgaagcg ctccctcgcg
     2881 atcgtcatca aaaagatcga gtacgcactg cgctcgtttc ccggcattgc gcgcgcgggt
     2941 cgcgtcgaca catatgccgg caactgcgag cagcgcaccg gcgatgagca ggcgctctgc
     3001 ctcacgttcg atccgcagcg ctccggcgac gtcttctaca ttcctgcgcg cggctggatc
     3061 ctcgagggcg ccgacgatca ctacgcgacc gcgcacggct cactcaacga ctacgaccgc
     3121 gacgtgcctg tcctgctcct tccgtttggc cgtacgccgc acacacccga cgcaaagccg
     3181 cgcgatcaga tcggcctgga ggaaatcgcg ccgctcgtca gcgcgtggct cggcgtcgca
     3241 ccgccgtgag gtaggatcgc gttggtgcga tggctctggc tcttgttggc ggggtgtggg
     3301 cggctgtcgt tcgacgcgct cggcgacggc gatgcgagca gcggcataga cacggcggcg
     3361 ccagacgcct gctcgttcag cgcattcgcg ccgccgacgc gcctgcccgg ccaggtccaa
     3421 tcggccgtcg acgattgggc gccgcgcagc gcgcttggca agacgcagat cttcttctgc
     3481 acgtacaacg gcagcgctgg cttgtgtgac atcgtctacg cgacgcgacc ggacaacacg
     3541 tcaccgttgt ccgcgccgat gttcatcccg gagctcgaca gcgcggcata cgaaggcggc
     3601 ccgtcggtca ccgaggatgc actcgacatg gtcttcttcc gcgacggcgc ggcgtacgac
     3661 ctcttcgaga cctcgcgcgc ttcgatctcc gatccgtggc agccgccgca acctctcgcc
     3721 atcaactcca ccatgagcga catgtcgccg tcgctcagcc ccgacgggct acgcatggtg
     3781 tttgcgtccg cgcgcgccgg ctccctcggt ggcggtgaca tctgggagac gacgcgcacg
     3841 agccgcaccg ccgcatggga cccgccggta caccgcatcg agctgtcgtc ggcgtccgac
     3901 gaaggcggcc ccacgatgtc gcccgacggg ctcgaagtct tcttcgcctc gaaccgcgcc
     3961 ggcggcctcg gtctcatcga cgtctacacc gctcgccggc tcgcactcgg caacccattc
     4021 ggcgcgccga cgctcgtgcc ggagctgtca tccacgcgcg acgacattag cctgagcatg
     4081 tccagcgacg ggcgctctct cttctacaac tacgattcgg tcagcgcggg cggccagaac
     4141 gccgacctct gggtcgcgac gcgcacgtgc aactagatct cgtgcggcat cggacggcgc
     4201 ggcgccttga agtagcgcag cggcgagtac gggccgaagt tcgacacgcg tgacgtgtag
     4261 agatcggcgt agtcgttgac ctgctcgccg aagcgcgagt tctcgttgcc ttcgcgcagg
     4321 cagctgcccc agtgcgagtt gtacgcgcga tcgatccgcg cctcgagcga gtcgacttcc
     4381 tgctccatca gcgccgcgcg atcgcgcagc gtatcgacgc tcgcacgcgc gagcttgcgc
     4441 tcttcttcga gacgggcccg cagcgcagcg gagcacgaat cgtcgtcgag cagtcgctga
     4501 attttcttga gacgcagcga ctgatagtcg atctccgact cgagattgcg atggcggcga
     4561 tcgagcagat ccagatcgcg aatctgtggg tcgagccgct cgatgaccga gatttcgcgc
     4621 tcgagctcct gcagcaccat cgcggtgcgc cacatgtgct gtttgcgcag gcgcaggatg
     4681 tcgccgtaga tgtggtcgcc gatgtagagg acctgctcgc ccttgatgcc ggtcatctgc
     4741 tcgaatgcga tgacgttgcc gccttgatag atcttgtcgc gcgagaggtg cttgatctcg
     4801 ccgttcgtcg cgaccggctg catcgtattc ggatcgatct gcacgaacgg ccgcagctct
     4861 tggaagaatg ccggcttcgc gccgccgacg atcacgatgt cgaagtagtt gcgccacgac
     4921 ggatacgctt tgcgctcacc gtcgagcagg tagctcatga tcacgctcgt gtagtcgtag
     4981 agcgagttcg tcagcaggaa gagcttcttg cccgagctgc ggaacttgtg cagcgtctcg
     5041 ccgagcagcg ggtccttcat gatgaaggcc gggatgtccg ctttgatcac ggacttcagc
     5101 gtgtcgtcgc gatgcgcttc gtcgatcgcg gtgcggatgt cgttgaacat cttctcgtag
     5161 tcgacggtgc cggtttggcg gtccgcccaa tcgacgagcg tcgtgtacat gacggcttcg
     5221 ggtagaccga acagggtgtc gatccactcg aagcgatccg ccgatagatt gatcttttcg
     5281 ttccgatacg tcgcacgccg ctggtcgtga tcgagctcgc gaaagccgtg gtagcagcga
     5341 ccgacgtacg cgtggcggtc catcttgaac acgttgccgt tcaagcgatc gaccatcagg
     5401 ccgcggatcg cccagcgcgg gtcgtacgag aggctgcgga tttcctcggg atatccgtgc
     5461 ttctcgacca gcttctggag cgtcagcgcg atcgagagct gctcgagctt gtcctggtgg
     5521 tacagcgcga gcgtgtagtc catgtcgaac ccgatcatct cgatcgagtc catccggagg
     5581 ttccgattgc agaacacccg gttcttgcgg gcgacgtcga tctcggtagt cgtctcgccg
     5641 agcaatgcgc cgatcgtcgg atcggtctcg aggctggcga ccattcgcga ttattgtagc
     5701 gcgattgcga tagagtcggg gacatggcct cgccgccccc tgcctggaac cgccccgacg
     5761 acccttttgc cgagctgatt tcggcggagg ccatcgcggc acgcgttgct gagctgggtg
     5821 ctcagatcac gaccgactac aggcccctca tcgggcaggc agaagtggtc gtcatcggtg
     5881 tgctcaaagg cagcatcatc ttcctggccg acctggtgcg tcacatcgct ttaccgatcg
     5941 catgcgattt catcggtatc tcgtcgtacg gcgacgcaac cgtctcctcg ggcgtggtcc
     6001 ggatcaccca ggacctcagc aagtcgatcg aaggcaagca cgtgatcgtc gtcgaggaca
     6061 tcgtcgacac cggccacacg gtgcactact tgctcgagaa cctcgcgacg cgccggcccg
     6121 ccaccatcaa gctcgcctcg ctgctgcaca agcccgagcg ccaagagcgg caagtgaaga
     6181 tcgactacct cggcttcacg attccgaaca agttcgtcgt cggctacggc ctcgacgtcg
     6241 cgcagcacta tcgcaatctg ccattcatcg gcttcgttca gaacgcatga aaccagacgc
     6301 gctggcaaag ctcccggcgg tgctcttcga gatcgggaaa ctgatcggat cggatctcga
     6361 tccgggcatc ctgctatcac gcattgcaga gctgctctgc cagctcgtga atgccaaggc
     6421 ctgctcggtg atgctgctcg acgcgaatcg caagcgcctg ctcgcaaagg ctgcgtacgg
     6481 tttgcggaca gaacggatgc acacgctctc gttcgcggtc ggcgaaggcg tcgctggctg
     6541 ggtcgtcgag cgcggcgagt cagcactgat cggcgatgtc tcgaaggacg cgcggttcgt
     6601 cgcgctgccg gccaatcaaa cgccgatagc atcgatgctg tgcgtgccgc tgctctcgcg
     6661 tggcgagcgc gtcggcgtcg tgacggctac ggccgagcac accgatgcat tcgataccga
     6721 agacctcgag atcgtccgct tcatcgcgac cacgatcgca ctcgatatcg agaacgtgcg
     6781 cctccaccgc gtcgccgtga ccgatccgtt gaccggcacg ttcaaccgcg agttcttgca
     6841 gcaacgcctg cccgccgaga tcgaggccac gatcgagcgt gaccgtccgc tctcgatcgc
     6901 gctcgtcgat gtcgatcact tcaaggccgt gaacgaccag tacggccacg gcgtcggcga
     6961 tgtcgtgctc gccgaagtag cgcgtcgcct gcgcagcgcg atccgcggtg gcgacctcct
     7021 cgtgcgctac ggcggcgaag agttcatcgc ggttctgccg aaggccgatg cgggccgcgc
     7081 gtgggaagtc ggcgagcgca tgcggcagcg cgtgtgcgag cgcgcgttcg atgtcggcga
     7141 tggcctcgcg ctgttgctcc gcatctccgt cggtatcgcg cagtggcgca tgggcgagaa
     7201 gatgccggat ctgatcgagc gcgcagacgt cgcgctatac ggcgcgaagc agcgtggacg
     7261 caatcgcgtc gaggtcgccc cgtgagcagc tccggggatc cgccgaagaa ggatcctccg
     7321 tcgaccgcgc cggccgctcc ggccgatgat cgcgagatcg gcagcgtcga cgagctctat
     7381 ggctttgcgc cggatccgtt catggattcg tacgaggagc ctgcgccgcc cgcggctccg
     7441 accgctgagc cgatcgcagt gaccgagccg gtcatcgcaa ccgtcaccgc agagccgctc
     7501 gccgcatcac cgacggacgt cgctccagct gccgtggaac cagtggacga gccactcctt
     7561 ccgacgacga ccgacgacaa tgcgttgcga gaagccgtcg gcattcccac gcccaagaag
     7621 cgcacgaccg cgaagatgga tgcggtcggc gacgacgagc ccaaggacaa gaaggtcaag
     7681 tacgtcgatc ccgatgcgtc gctttggtcg cgcaagctgc tcacgctgat cgccgtcggc
     7741 gtgctcgcga tcatctcgat cgctgtgctt gtcatcctcg gcagcgtgaa tagtcgccgc
     7801 tacgagttca cgtgctcgtc cgatcgcgtc ggcgcagagc aaggccgctc attcccgccg
     7861 tggggcaccg agcagatgac tggccccgag tggaagccga tcgcactgcc gccgaacgcc
     7921 gagtgcacgc cccgcgacac ggagaacaag tccgagctcg aaggctggta tctgcaagca
     7981 ctcgtcgatc gcgcgacgac gacgctcacc acgccgaacc tcgtcgagca aatcaccgcg
     8041 gcgcccgcgg gcaagggcgg caacgcgctc gacatcgtcg cagctcagct ccagcaagcg
     8101 ctgctgctct cgcgttcggc cgatcatcgc gatcaacgca aggaggtcga gcgcctgatg
     8161 ggcgacgtgc agtactggcg tgcgtcgctg cggcttcgcg atgcgtcggc ggcgctgctc
     8221 gatgcgtcga agcaattcga gaccgcagcg cagcagcgtc ctcgtcatgt caccgatgcc
     8281 gccgcgtggt cgcaatacgt gaagaagctc gcagacgatc tgcgcgcagg tccgaacggt
     8341 gcacccgcag tacagacttt tccgccgacg ccgacggcga cgccgccgac cggcgatcgt
     8401 ccgagtgcgc cagcgggcgt tgcgttgccc gtcgagacac caccgtcgca gcatgatgag
     8461 actccgccgc cgacacccga cgcaggtgtt ccgaccggcg gcgtgttgct ctaggcgcgc
     8521 ggcgtgcacc gcctcctctc ggaggaagtc atgacccgca acgtcgcgat gatcgtcgga
     8581 agtcttcgca aggattcgta cacgcgcatg gtggcgaacg ctctcgccga gctcgctccc
     8641 aaatcacttc atctcgacca cgccgagatc ggaaatcttc cgctctacaa ccaagacctc
     8701 gacgccgaga accgcgcgcc tgccgaatgg acgcagttcc gcgatcgcat gcgcgacgac
     8761 gccgcggtcc tgttcctcac gcccgaatac aaccgctccg tccccggcgt cctcaagaac
     8821 gcgatcgatg tcggctcgcg tccctacggc cagagcatct ggtcgggcaa gccggcggcg
     8881 atcgtgagtt gctcgcccgg catgtacggc ggcttcggcg cgaatcatca ccttcgccaa
     8941 tcgctcgtgt tcctcgacat gccatgcctg cagcagcccg agatgtatct gtctcgcgtc
     9001 gataaagcgt tcaaggacgg caagctcgtc gacgacaccg tgcgcgagct cctcacgggc
     9061 ttcatcacga agttcgagaa ctgggtcgac aagtacgtca cctacgtcgc gccgtcgcag
     9121 aagaagtcgg cgtagtcgtc tgctacgctt gatcgggtgc gagccccgat atcgctgatc
     9181 ctcgtcgcgt tgttcggctg ccgcgaaggc aaggagacca agccggtcat cgccgatcat
     9241 cacacggcgc cgccggtcac cgcgcccgtc ccgccgacgg cgacgcccga tccgtgggcc
     9301 gcaggtccgg cagactcagc tgacgacccg ccgtcgctcg ccgagcgcca caagcgcgcc
     9361 gaagaagcgt gcccgctcgt caaggcgccc tacttctaca gcatcgagaa ggcgggcaag
     9421 gtctcgcaca tcctcggcac gcgccacatc tcggtgccgc tcgcgaagtt tccgaaggaa
     9481 gtgaccgacg cgatatcgac cgcaaagctc gccgtattcg aggtcgcgcc cgacgacaac
     9541 tccggtgtcg agacgccaaa gatctcgatc aagaacgagc tcggtcccga tctctggaag
     9601 aaatacgaag cgctcgtcgg caaggccgcc gccgaatcgc tcgaggaagc cacgcccgcc
     9661 gaagccatga tctggctcat cgcgatgtac gaggacatcg gcgcgatgct cgactccgag
     9721 atccaacgcc aagtccaggc agcgaagatt ccgacgatgg gcctcgagac gagcgcgttc
     9781 caggacacgc tgctccagaa gctgctcgac atccgcatgc tcaaagccga ggtcgcccac
     9841 accgatggcc gcgacgagct cgcgaaagaa tcgaaggaag acctcagcga gtactgcgcc
     9901 ggctccgacg acaaaccggg catggacgac gacacgcgca aggacttgct cgaagcggga
     9961 tacaccgcgg ccgagatcga ttcgatcgat gaccagatgg tgtatcagcg caacgcgagc
    10021 tggatcccga agctcgagaa gctgttcgcg aaaggtgacg tgttcgtcgc cgtcggtgcc
    10081 gaccacctca cgggcgatcg cggtgtcgtc gcgctgctcg cgaagcgggg cttcaccgtc
    10141 aagcgcgtac cgcgcaagta gcctcgcggg aggcgtcgag cctagcgccc ggcggtcgtc
    10201 gcgaagtaga cgccgatgag ctcgaacgcg ccgtcgcgct gccaatcgat accgacgccc
    10261 cagccgacat cttttccagg tcgcacgatg cccgctcgcg cgcgtgcacc acgattcgag
    10321 atgccggcgg ccagctcgat gccgcgaaca cccgggccgt cctccgaggc gaagcgcccg
    10381 atcgcggcga taccctcgac gcccgcgtag ccgtgcgccc cgaagttgct gccggacatc
    10441 gcagcatccg tgccgccgcc gagcgtgaat ccgagcggcg cgacgagcgc gacagccgcg
    10501 cgatcgctga tcgttcgtac gacgccgatc aacagctggc cctccgtccc aaacgcccct
    10561 gcgccatcgc cgccgagcgt gaacctaatg ccgcccgcaa tctttgtggc gccctctccg
    10621 ttcgctccga tcatgtcgac gccgaaccgt ccgccgagat gtggctcgtc tccgccgcgc
    10681 acgatgtacg gctcgtacag aaatcgcccg agcacgacgt cgtgtggtgc gtcgacgatg
    10741 acgcccgtcg aatcgctggc ggcagcgggc gccggatccg tagcgatcgt tgcagcacgc
    10801 gcggtgcgcg gcgacacgat gcccgcgatc acgacgagca ccacgaacac cgcgatcagc
    10861 acgagcacga tgaatcgcat cacgcgtgat caacggagat cgcatcgcgc gcttacaggc
    10921 acggcagttg ctgtgcaggc tcgccatgcg tatccgattc gaccgtggaa ccctcgtgat
    10981 cgaccgctgc gaaagcggct tcgatcgtac tcatctccgc gatgccactt gggatcaaga
    11041 cctgtgtgca tggcgacttc ctgccgagtg cttgtccgaa ttgcgtggcc ggctctcggc
    11101 cagcggcgtc cgtcattcgg acgaaaccgg acaagcgcgt tcgagcttga atcgcgagag
    11161 ctggtcgatc ccgccgttgc gcgactatca gcgcgcggcc gttcaagcgt ggcgcgacgc
    11221 aggagatcgc ggcgtcatcg tcctgccgac cggcgctggc aagacgatcg tcgcactcgc
    11281 agcgatcgcg gagctcgccg tgtccacgct cgtcctcgtc ccgacgcgca tcctcctgga
    11341 tcaatgggcg cgcttttttg gcatgtgctg ggcgcatccc atcggtcgtc tcggtgatgg
    11401 tcaacgctcc atcgcgccga tcacgatcgc gacctacgcc agtgcgatca cgtgggcgcc
    11461 gcgcatcggt gacagcttcg ggctcgtcgt cgtcgacgaa gcgcatcacg tcggcgcgtg
    11521 gtgcccgcgc gagatcctcg agatgctcgt ggcgccttcc cgcctcggcc tgacggcgac
    11581 acctcccgac ggcgtcgcgg caagcgccct cgcccgccac gtgggaccga cggtcttcga
    11641 atgcggcatc gccgacctca cgggcactgc actcgcgccg tacacgcacg agacgatcgc
    11701 gatcgagctc accgccgaag agcgctccgc gtaccgctcg acgcggggcc tgttcgccgc
    11761 ctactacgcg cgcgcgcgcc gcgatgaccc gaacatgcac tggcgccagt tcgtgcacga
    11821 ggcgcagaag tccgacgatg gccgcgaagc cgtgctcgcg tggcgagcct cgcgcacgat
    11881 cgtctcgtac cccgaggcca agcgctcgaa ggttcgcgag ctgctcgcgc ggcacgctgg
    11941 ggaccgcgtc ctcgtcttca cggccgacaa cgccacggcc tacgcgatct cacgcgagct
    12001 gctcgtcccg ccgatcacct gcgacatccg gcgcaaggag cgcaccgaga tgctgtcgcg
    12061 cttctcgtcc ggcgcgtgca acgtgctcgt ctccgcgcaa gtcctcgacg aaggcttcga
    12121 cattcccgac gcagacgtcg cgatcgtcgt cggtggcacg tcgtcgcagc gtcgtcaggt
    12181 gcagcgcatc ggtcgcgtcc tgcgcccgcg gccgggcaag cacgcggtcg tctacgagct
    12241 ggtcgtcgag ggcaccgctg aagtccgcca gtcattcgat cgcgcgacca accgctccca
    12301 tcgcggtgcg cgtcgtcatc ccgaaatcgt ccgccaggac gcgctctcgt cgcaggaggt
    12361 gtggtcgtga agctgctcac tgaaacagat ctgtcgtgga tcgccgacat gctcgacgtt
    12421 gtcgtggcgt gccaaggcca accgtggcgc accgcgctcg aacgtctcga aggcctcgcg
    12481 cccgagctgg agtcgtcgtc gtcgcaacgc gtctcgcaga aacgcgtcgc cgccgtcctc
    12541 ggggcgatcc aacgcgtcct cggccggtcg acgaaagccg atctcgcaag gacatgccgc
    12601 gcgctcgtgc tcgggcgtcc cgcgctcgag ccggctgaac gcctcgaccg cattgccgca
    12661 gctgcccggt cactcgacat ctcggtcgcc acggtcgagc agctgctctg gatcgacctg
    12721 ccgcgcgagc gccctgtcga gcttccgttc ggtcgtcccg acgagctcga gatcgcggcg
    12781 ttcgcgaaca tgcagctcat cgagaagacg ttgcgacgcg cgcaaacggc gcagctgcga
    12841 atccgcggcg acgcaggccc gttcctccgc ggtgccgctc gttgcggcct gctcacgact
    12901 gcgcgccctg agcccgtcgc gcctcctgtg cccgtcgcgc ctcccgaaga tcggacgacc
    12961 acccgagagg atatcgacca cctgcatggc gccatcgagc aggcgaagcc cgtgcgggtt
    13021 gagcccgtgc gggttgagcc cgtcacgctg atcgacatcg ttgggccgct cgcgctgttt
    13081 catcgcaccg cggtgtatgg ccgtgcgctc agcgcgctgg ttccgttgct tgccgaggtc
    13141 tcggcgttcg agctggtcgt catgtcgacg ggcttcaagg gcgaatacgc cgtcgagctc
    13201 ggctcgcccg cgctgctacc tcgaacgtcg gcggcgctgc acggcccatc aaagcaagcc
    13261 gctcggctcg cgcgcgagct ccgcgagctg ctgccggaca ggcgcatcac catcgcgccg
    13321 cccgctgtcg tcgccgacgg tgcgtaccta tgccccgatc tcctcgtcgg cgagacgtac
    13381 gtggagctcg tcggcttctg gaccgcagag cacctcgcgc gcaagcgagg cgcgtacgag
    13441 gccgctggcc tgcgggtagc gctgtgcgca gacgagttgc gcggctgcgc gggcgaagag
    13501 ctaccgccgg acgtcatcgg ctacacgaag tacgtcgatg ccgatgtcgt cgcgcggcgc
    13561 gtggacgcga ggcgaatcgt gccgcgctag cccgacgtac cgatcgcgag gcgcgtgagg
    13621 cccgcttgct tcgcgcgctc catcagattc acgacgcggc cgtgctgcac gcccttgtcg
    13681 gctttcagca cgacctgcgt gttcttgtcg cgagcgaacg cggcgcggaa caggttatcg
    13741 aggtcggcgt ccgggatccg cttgcccccg acgtcgacgt caccttgcag cggcaccgtg
    13801 atcacgagcg actgcttcgt cgtgtcgatc tcttgggcct ggccttgcgg caggttgacc
    13861 ttgaggccgg acttcttctc gatctccgac tgctcttgcg cctcggcgac cttcttcttc
    13921 tcttggcggg cttccacctg cacggcgagc gcgctcacca tgaagatgat gacgaggacg
    13981 aggaagacgt cggtgagagg cgtgatgttg atctccgcga agatcccatc gctgccgccg
    14041 tcttcgtctg cgtcttgatc aggcagcttc ccgagagcca tcgccgtcgc ctttcgtgcg
    14101 ggccttcgta tcgctatccg cctttgcttc gtcgacgcgc ttgcccttcg gctgttgatc
    14161 gcgcggctga tcgagcagga gctcgaggaa ttcgtcggtg agcagcttca tctcggtcgc
    14221 gatgcgactc gcgcgctgcg agaagaagtt gaataggatg actgcttcga cggcgacgag
    14281 aataccggcg gccgtcacga cgagcgcttc ggagatcggc ccggagacca tcgagaactt
    14341 cacttcgtcg tcgcccttga agccgcccat cgcgctcatg atgcccagga cggtgccaaa
    14401 gagaccgacg aacggagcgg tcgcgccgat cgtgccgagc atccagaggc gcgagcggag
    14461 atcttgattg acgcgcatgc gctcgcgatg aac
//
LOCUS       DATLBZ010000351         9654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4118339, whole genome shotgun sequence.
ACCESSION   DATLBZ010000351 DATLBZ010000000
VERSION     DATLBZ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9654
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4118339"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VMZ53_18105"
     CDS             complement(<1..349)
                     /locus_tag="VMZ53_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS30431.1"
                     /translation="MLKDKVIVVTGASRGIGEAIARAAIDNGAKVVLASRKQADLDKV
                     AGSLPADRVIAVACHTGKAEDVDALFARALDKFGRIDGAVNNAATNPYFGPLIDTPDA
                     AIDKTFEVNVRGYL"
     gene            complement(379..1350)
                     /locus_tag="VMZ53_18110"
     CDS             complement(379..1350)
                     /locus_tag="VMZ53_18110"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUS30432.1"
                     /translation="MKGLATVERGVELVERAVPVPQRGQVLVKMRAAPINPNDLMFLD
                     GVYEIKKPLGTIAGFEGTGTVVGTGGGAMGRFLLGREVACAAGDGDGTWAEYAVADAM
                     KCAPLRKGTDALQGAMLLTNPLTASVLLETARRAGHRAFVQNAAAGALGKMLVRLCAR
                     EKLPLVNIVRRAEQADALRAIGAEHVVVSTDADAAEKLRALCERLGVTFAFDAIAGEA
                     TDQLARAIGLEGRILVYGMLSGKPCQIDADTLVFRRLRVDGFTMYAWLEHTSLLGKLR
                     TLGAAQRRLADDLASEIRATKSLAEYADALALARGSTSEGKVLFAHR"
     gene            complement(1387..2313)
                     /locus_tag="VMZ53_18115"
     CDS             complement(1387..2313)
                     /locus_tag="VMZ53_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30433.1"
                     /translation="MARNSGWLLLLCACGSVASEKQDAAPAVDSKVIDAAPDTTGPRC
                     DPTKAFANLAPLANVNSASSELWGRMSADELTLYFGSNRAGGAGGYDIYTATRSSTTA
                     DFGSPTQVASLATAGDDRSPMPTANGLGMYFSRSPAGQPTVTDFYLATRTSTSTDFTQ
                     SALVPELNSGSEETELYLTEGENVMYFVSTRTPSQSYDIWRATKQTNGVWGQLEIVTA
                     ASGASIEGFPIGSEDGKALYFGSNRTGGSLWQVTRPTAGGSFGAASVVTELETSDDEA
                     PGWLSKDGCVIVFHRNGTGLGQYDLWMAKRPL"
     gene            2413..4239
                     /locus_tag="VMZ53_18120"
     CDS             2413..4239
                     /locus_tag="VMZ53_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HUS30434.1"
                     /translation="MASQQNYYKANLRDLSFLLFEQFHLEDLLGKEPYANWGKDEVLA
                     VLEEAYGWVQKYLGPINGVGDEVGCKLENGQVKVPPGFKEAWQELFKAGWRTLAIEES
                     HGGQAGPFTLAMMVEEFMCGSCTSFNMYPALTQGAADVILAFGTDAQKATYVENMFNG
                     KWAGTMCLTEPHAGSDVGSASTTAKLRDDGKYNIQGTKIFISGGDQDITDNIVHMVLA
                     RTPEAPPGTKGLSLFIVPKLRPDGTPNDVKTAGIEHKMGIKASSTAQLVFGEDGNCIG
                     ELVGTTEQKGMSQMFHLMNYARIGVGIQSLSIASSAYLNALEYAKDRKQGSSIKQWKD
                     ATAPRVPIIEHADIRRMLLDMKSRVEGIRALVVKLTMHIDRVNALQKTGGEQKDIDYH
                     QGQVDLLVPLVKSYGSDQAFSICSTAIQVYGGAGFLKDWPVEQYARDSKIFSIYEGTN
                     HIQAMDLVGRKLMQRGGANVQGFAADVGKFVAAHKDHASLKDSVAVLAQAMEAMTATG
                     GKFMQWFGGGKLEMVPAVANRFLEMMAETTIGWLLLEQAVIAEQALGKLAADHPDKAF
                     YTGKTYAAKYFALNVLPGVVAKAQLIAREDRSAVDMPIDAFA"
     gene            4226..5335
                     /locus_tag="VMZ53_18125"
     CDS             4226..5335
                     /locus_tag="VMZ53_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30435.1"
                     /translation="MRSHNAIVAVLVAACGSTTPVQPGAPAPTSVPPPPAVPTCEEAG
                     IILRGPIEASQWEKAGPKREAAIANACKGDRWSQSTITCVASSTNPLECLDALTAAQR
                     ASYDAALAKWSKEFGEPEVALPLTCIDALHSPQLLDPPLTDASPQREWELGARQRFLP
                     AMCMDDNWSDEALGCLRDATTEVATGACVMKLDPKVHARLVTVHDLARAIDALRKTPA
                     KLDCAKVAEHHYSDEKTKRDLKSMKPADRKRLVAGVRKTMSDACSNEGWDEETRACML
                     ADGGEENCFGTVRFATSAGQPRPMISIPECGEYARAVSELQRCSAAPPETRKAMQEAF
                     DSVDRSATADGKEALVSACRNAADSIRQVIAAYGC"
     gene            5384..6247
                     /locus_tag="VMZ53_18130"
     CDS             5384..6247
                     /locus_tag="VMZ53_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30436.1"
                     /translation="MRALLVVLVVACGGSQGTKQDPVAARSVPRATAPDAPAPRSAVA
                     GSPDCDETARGFELFDDATDPEWQETFRVRAIANLCTYQEWPEGVRTCLRDASTEASR
                     AACVAALDSHQVARVSDLAARAKSIQRLRVSPAAITCERVAENVHSEMVWTNLVDEPA
                     ERATKIAAVRREMTDACRQERWNASTRACLISGAGEHVCLVGTTWSPWRMARRLPVEC
                     RELRRAIDASKTCRAMTQGDRDKVTDWWHVVLAEVKDDEESAQLAMLPELCDAYGFRA
                     RKKLGAAGCTY"
     gene            6430..7161
                     /locus_tag="VMZ53_18135"
     CDS             6430..7161
                     /locus_tag="VMZ53_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30437.1"
                     /translation="MEQATCTDALHAVELFDPPLDDRSPEREWQLQSRRFVMMCEGWS
                     TVEKNCLRDATTPDATNACVTLAPEIHARLLSLSSLAASIADLRRSLDKIDCTTVAVT
                     HNSEAFWNATFDREGLKRADRKALLDGARYTMASACVDEQWDDTQRACMIAGGGEMLC
                     LRTRTPAWDASYIGLTPPADCREYQRAEQALQKCPAVSSEDVDALTGALDTWDEGSGD
                     QRQAIARMCKTYVSKIRATLTAAGC"
     gene            complement(7158..7502)
                     /locus_tag="VMZ53_18140"
     CDS             complement(7158..7502)
                     /locus_tag="VMZ53_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30438.1"
                     /translation="MWRFVCLLLLASCQPKQPLQPSFFHAKPVEYASCQDTVTCYARC
                     NPVTEPCMQACDRNALADDAQHARDLTSCLQNTRCRDEACTREQCGTQIDTCNNAREK
                     VPGQFQTQGGPT"
     gene            7659..>9654
                     /locus_tag="VMZ53_18145"
     CDS             7659..>9654
                     /locus_tag="VMZ53_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HUS30439.1"
                     /translation="MRTSAATRLATYVLAAAACLPLGACSDELEITPLYNPANGRVVI
                     QTNQDISGKQLYVQVRRGKFGVLDCKQMAANLTAVEELSGNRIDGPFVDPQLTKPFYG
                     GPEWMNPTAQMIAQAEAGTDSIIDVCLMDGSKVVVQMERDLFRAWDDARKQGIGGKAD
                     NPESGEQYINSPVAYGERCVAELGEIPFFEKEADGKYSTYDCIESTEIPMTVTKADGS
                     VDKPREGTVNQCDNPQYIYSLCEAGPRVAQRTNEQGTRWVLLCRKSKGGYASNQYNDI
                     AMIGSNPFTGKTCFFQNALYSKTDGGHVPHPADKEKSQNLWSGVHGGLGSGIECSTCH
                     DADPFIHTPWIDGAKDPQGRPIVPKMGIDADLAIGANDTPYSLINAKGQGWKYERQIV
                     SPEASACLKCHRMGSGRWTESWISRLGGTDSSWTGITTAAFNQSAHKYWMPPDVAMDT
                     ETMWTSSVFKTALDFIESCGKNQSNPACIWKDIPTKLASDTAGGALRNKVTLADDELA
                     KQATTIIGMNKNTPSQICAECHAPNQTTLRDWQEKTDTAMSTCLKNVDDGGVATTAKE
                     DNVSIGKDEFKTFGPYEVALGGKIEVKMAGTGDADLYVKRGAEPTVDSYDCRPFSREP
                     NEQCVGTQFNANGPAKFWVGANGFADSSKITITVTYDKPGA"
BASE COUNT         1831 a         3153 c         3107 g         1563 t
ORIGIN      
        1 agaggtagcc gcgcacgttg acctcgaacg tcttgtcgat cgccgcgtcg ggcgtgtcga
       61 tgagcgggcc gaagtagggg ttcgtcgcgg cgttgttcac cgcaccgtcg atgcgaccga
      121 acttgtcgag tgctcgcgcg aacaatgcgt cgacgtcctc ggccttgccc gtgtggcaag
      181 ccaccgcgat cacgcggtcg gcaggcagcg agcccgcgac cttgtcgagg tcggcttgct
      241 tgcgcgacgc gaggacgacc ttggcaccgt tgtcgatcgc cgcccgtgcg atggcttctc
      301 cgattccgcg gctcgcgccc gtgacgacga tgaccttgtc cttgagcatg cgcgcaacct
      361 accagaacgg tcatgccatc agcggtgcgc gaacagcact ttgccttcgc tcgtcgagcc
      421 gcgcgcgagc gcgagcgcat ccgcatattc cgcgagtgac ttcgtcgcgc ggatctccga
      481 cgcgaggtca tcggcgagcc gccgctgcgc cgcaccgagc gtgcggagct tgccgagcag
      541 cgacgtgtgc tcgagccacg catacatcgt gaagccatcg acgcgcagcc ggcggaacac
      601 cagcgtgtcc gcatcgatct gacacggctt gcccgacagc atgccgtaca cgaggatccg
      661 tccttcgagg ccaatagcac gcgcgagctg atccgtcgcc tcgcccgcga tagcgtcgaa
      721 tgcgaacgtc acgccgagcc gctcgcagag cgcacgcagc ttctcggctg catccgcgtc
      781 agtcgacacc acgacgtgct ccgcgccgat cgcgcgcaat gcgtctgctt gctccgcacg
      841 acgcacgatg ttcacgagcg gaagcttctc cctcgcgcac agtcgcacca gcatcttgcc
      901 gagcgcaccg gccgcggcgt tctgcacgaa tgcgcgatga ccggcacggc gcgcggtctc
      961 gagcagcaca ctcgcggtga gcggattcgt cagcagcatc gcgccttgca gcgcgtcggt
     1021 acccttgcga agcggcgcgc acttcatcgc gtcggcgact gcatactccg cccacgttcc
     1081 gtcgccatcg cccgccgcac acgcgacctc gcgcccgagg agaaagcgac ccatcgcacc
     1141 tccgcctgtg ccgacgaccg ttcctgttcc ctcgaagccg gcgatcgtgc cgagcggctt
     1201 cttgatctcg tacacgccgt cgagaaacat cagatcgttt ggattgatcg gcgcggcacg
     1261 catcttcacg agcacctggc cgcgctgcgg caccggcacg gcgcgctcga ccagctcgac
     1321 gcctcgctcg accgtcgcga gccccttcat gcgttctggg agcgtatggc tcgagagccg
     1381 cttcacttag agcgggcgct tcgccatcca gaggtcgtac tgaccgaggc cagtaccgtt
     1441 gcgatgaaac acgatcacgc agccgtcttt gctcagccag ccgggcgctt cgtcgtcgct
     1501 agtctccagc tcggtgacga ccgatgcagc gccaaacgag ccacccgccg tcggacgcgt
     1561 gacctgccaa agcgatccgc ccgtccggtt ggatccgaag tagagcgctt tgccgtcttc
     1621 actaccgatc ggaaaccctt cgatcgatgc gccgctcgct gcggttacga tctcgagctg
     1681 cccccagact ccgtttgtct gcttcgtggc gcgccagatg tcgtagctct gactcggcgt
     1741 gcgcgtgctc acgaagtaca tgacgttctc gccctccgtc aggtacagct cggtctcttc
     1801 cgatcccgaa ttgagctctg ggacgagtgc ggactgtgtg aagtcggtcg acgtgctcgt
     1861 gcgtgtggcg agatagaagt ccgtgaccgt cggctggcca gcaggcgaac gcgagaagta
     1921 catcccgagc ccattcgcag tcggcatcgg gctgcgatcg tcgccagcgg ttgcgagcga
     1981 cgcgacctgc gtcggcgatc cgaagtccgc ggtcgttgac gagcgggtcg ccgtgtagat
     2041 gtcgtagccg ccggctcctc cggctcggtt gctgccgaag tacagcgtga gctcgtcggc
     2101 cgacatgcgt ccccacagct cgctcgatgc cgaattcacg ttcgcaaggg gcgcgagatt
     2161 cgcgaaggcc ttcgtcggat cgcagcgcgg gccggtggtg tcaggcgcgg cgtcgatgac
     2221 ttttgagtcc acggccggtg cggcgtcctg cttctcgctc gcaacgcttc cgcacgcgca
     2281 caacaacagc aaccacccag agttacgcgc catcccgcag atgatatcgc gtaactcctc
     2341 gtcgatgctt gacgcggcgc attagtggcg ccataaaatg aatgacggtt cattcagtag
     2401 gaggacccgc ccatggcgtc gcagcagaac tattacaagg ccaacctccg cgacctctcg
     2461 ttcctgctgt tcgagcagtt ccatctcgag gacctactcg gcaaggagcc ctacgcgaac
     2521 tggggcaagg acgaagtcct cgcggtgctc gaggaagcat acggctgggt gcagaagtac
     2581 ctcggcccga tcaacggcgt gggcgacgag gtcggatgca aactggagaa cgggcaggtg
     2641 aaagttccgc ccggcttcaa agaagcatgg caggagctgt tcaaggccgg ctggcgcacg
     2701 ctcgcgatcg aagagagcca cggcggtcaa gcgggcccat tcacgctcgc gatgatggtc
     2761 gaagagttca tgtgcggctc gtgcacgtcg ttcaacatgt atcccgcgct cacgcagggc
     2821 gccgccgacg tgatcctcgc gttcggcacc gacgcgcaga aggcgacgta cgtcgagaac
     2881 atgttcaacg gcaagtgggc tggcacgatg tgcctcaccg agccgcacgc aggcagtgac
     2941 gtcggcagcg cgtcgacgac cgcgaagctg cgcgacgacg gcaagtacaa cattcaaggc
     3001 acgaagatct tcatctccgg cggcgaccaa gacatcacgg acaacatcgt gcacatggtg
     3061 ctcgcgcgta cgcccgaagc acctcccggc acgaaaggcc tctcgctctt catcgtcccg
     3121 aagctgcggc ccgacggcac gccgaacgac gtgaagaccg ctggcatcga gcacaagatg
     3181 ggcatcaagg cgtcgtcgac ggcgcagctc gtgttcggcg aagacggcaa ctgcatcggt
     3241 gagctcgtcg gtacgacgga acagaagggc atgtcccaga tgttccacct catgaactac
     3301 gcgcgcatcg gcgtcggcat ccagtcgctg tcgatcgcgt cgagcgcgta cctcaacgct
     3361 ctcgagtacg ccaaagatcg caagcagggc agctcgatca agcagtggaa agacgccacc
     3421 gcgccgcgcg tgccgatcat cgagcacgcc gacatccgcc gcatgctcct cgacatgaag
     3481 tcgcgcgtcg aaggcatccg cgcgctcgtc gtgaagctga cgatgcacat cgatcgcgtc
     3541 aacgcgctgc agaagaccgg cggcgagcag aaagacatcg actaccacca aggccaagtc
     3601 gatctgctcg tgcccctcgt gaagtcgtac ggcagcgatc aggcgttctc gatctgcagc
     3661 accgcgattc aggtctacgg cggcgccggc ttcctcaaag actggcccgt cgagcagtac
     3721 gcacgcgact cgaagatctt ctcgatctac gagggcacga accacatcca agcgatggac
     3781 ctcgtcggcc gcaagctcat gcagcgcggt ggcgcgaacg tccaaggctt cgccgccgat
     3841 gtcggcaagt tcgtcgccgc gcacaaagac cacgcctcgc tcaaagactc ggtcgccgtc
     3901 ctcgcgcaag cgatggaagc gatgacagcg acgggcggca agttcatgca gtggttcggc
     3961 ggtggcaagc tcgagatggt gccggccgtc gcgaaccgct tcctcgagat gatggccgag
     4021 accacgatcg gctggctgct cctcgagcaa gctgtgatcg cggagcaagc gctcggcaag
     4081 ctcgctgcgg atcacccgga caaggcgttc tacacgggca agacgtacgc agcgaagtac
     4141 tttgcgctaa acgtactgcc gggtgtcgtc gccaaggccc agctcatcgc gcgcgaagat
     4201 cgctcggcag tcgacatgcc gatcgatgcg ttcgcataac gcgatcgtcg ctgtcctcgt
     4261 cgccgcatgc ggcagcacga cgcccgtgca gcccggggcg cccgcaccga cgagcgtccc
     4321 accgccgccc gccgtcccca cctgcgaaga ggccggcatc atcctgcgtg ggccgatcga
     4381 ggcatcgcaa tgggagaagg ccggccccaa gcgagaagcc gccatcgcga atgcatgcaa
     4441 aggcgatcgg tggtcgcagt cgacgatcac gtgcgtcgcc tcgtcgacga atccgctcga
     4501 gtgcctcgat gcgctgaccg cggcgcagcg cgcgtcctac gatgcggctc tcgcgaaatg
     4561 gtccaaggaa ttcggcgagc ccgaagtcgc gttacctctg acgtgcatcg acgcactgca
     4621 ctccccccag ttactcgatc caccgctcac ggacgcctcg ccacagcgcg agtgggagct
     4681 cggcgcgcgc caacgtttcc tgccggcgat gtgcatggac gacaactgga gcgacgaggc
     4741 gctcgggtgc ctacgcgacg cgacgacgga ggtagcgacg ggggcgtgcg tgatgaagct
     4801 cgatcccaag gttcatgctc ggctcgtcac ggtgcatgat ctcgcgcgcg cgatcgacgc
     4861 cttgcgcaag acgccggcga agctcgactg cgcgaaggtc gccgagcatc actacagcga
     4921 cgagaagacc aagcgcgatc tcaagtcgat gaagccagcc gatcgcaagc ggctcgtcgc
     4981 cggcgtacgc aagacgatga gcgatgcctg tagcaacgaa ggctgggacg aagagacgcg
     5041 cgcgtgcatg ctcgctgatg gcggcgaaga gaactgcttc ggcaccgtcc gctttgcaac
     5101 ctcagccggg caaccacgcc cgatgatctc gattcccgaa tgcggcgagt acgcacgcgc
     5161 ggtgtctgag ctgcagcgct gctctgccgc accgcccgaa acccgcaagg cgatgcaaga
     5221 ggcattcgat agcgtcgatc gctcagcgac ggcggatggc aaggaggcgc tcgtgtccgc
     5281 atgtcgcaac gccgccgact cgattcgcca ggtcatcgcc gcgtacggct gttaatgaag
     5341 ctcagctcga caagccgcag acgcgtgtgc atagagatct gtcatgcgcg cgctgcttgt
     5401 cgtgttggtc gttgcgtgtg gtggatcaca gggaacgaag caagatccgg tagcggcgcg
     5461 atccgtaccc cgtgcgacag cgcctgatgc accggcgccg cgatcggcag tcgctggctc
     5521 gcccgactgc gacgagactg cacgcggctt cgagttgttc gacgacgcga cggatcccga
     5581 gtggcaggaa acgttccgcg tgcgtgcgat cgcgaaccta tgcacgtatc aagaatggcc
     5641 ggagggcgtg cggacgtgcc tgcgtgatgc ctctaccgag gcgagccgtg ccgcgtgtgt
     5701 cgcagcgctc gattcgcacc aggtcgcgcg tgtttcggat ctcgcagctc gcgcgaagtc
     5761 gatccagcgc ctgcgcgtgt ccccggctgc gatcacctgc gagagggttg ccgagaacgt
     5821 tcacagtgaa atggtatgga cgaacctcgt cgacgagccc gccgagcgcg cgacgaagat
     5881 cgcggcggtc cgccgcgaga tgaccgacgc ctgcaggcag gaacggtgga atgcctcgac
     5941 gcgggcgtgc ctgatctccg gcgcgggtga gcacgtatgt ctagtcggta cgacatggtc
     6001 gccatggcgc atggcgcgtc gcctcccggt cgagtgtcgc gagctgcgcc gcgcgatcga
     6061 cgcgtcgaag acgtgccgcg caatgacgca gggcgatcgc gacaaggtca cggactggtg
     6121 gcacgttgtg ctcgccgagg tcaaagatga cgaagagagc gcgcagctcg cgatgctccc
     6181 cgagctgtgc gatgcgtacg gctttcgagc gcgcaagaag ctcggcgctg ccggttgcac
     6241 ctactgaatg gtggtcgcgc tgaatgtcac cgacgtcgtt gcgtcggagg tctcatgcgc
     6301 caactcgtga tcgtcctgct tgtggcgtgc ggttcgcggc ccacggagcg agccatcgcg
     6361 aaccatccgg caccaccacc accgaacgaa acacgcgagc cgcggcccaa ccgcgttgcg
     6421 cccgccaccg tggagcaggc cacgtgcacc gacgcgttgc acgcagtcga gctcttcgat
     6481 ccgccactcg atgaccggtc gccggaacgt gagtggcagc tgcagtcgcg ccgcttcgtc
     6541 atgatgtgtg aaggctggtc tacggtggag aagaattgct tgcgcgatgc cacgacgcca
     6601 gacgcgacga acgcatgcgt gacgctggcc cccgagattc acgctcgact gctctcgctg
     6661 agcagcctcg cggcgtcgat cgccgacctt cgtcggtcac tcgataagat cgactgcacc
     6721 accgtcgctg tgacgcacaa tagcgaggca ttctggaacg caacattcga ccgcgaaggg
     6781 ctgaagcgcg ccgatcgaaa agcgctgctc gatggcgctc gctacaccat ggcgtcggcg
     6841 tgtgtcgacg agcaatggga cgacacgcag cgcgcgtgca tgatcgcagg cggcggcgag
     6901 atgctgtgcc tccgaaccag gactcctgcg tgggatgcaa gctacatcgg gctcacaccg
     6961 cctgcagatt gccgcgagta ccagcgcgcc gaacaagcgc tgcagaaatg ccccgcggtc
     7021 tcgagcgagg acgtcgacgc tctcaccggc gcgctcgaca cgtgggacga aggcagcggc
     7081 gatcagcggc aagcgatcgc gcgcatgtgc aagacgtacg tgagcaagat ccgcgcgacc
     7141 ctgacagctg caggctgcta ggtcgggccg ccctgcgtct ggaactggcc gggcactttc
     7201 tcgcgcgcgt tgttacacgt gtctatctgg gtgccgcatt gctcgcgcgt gcacgcctcg
     7261 tcgcggcagc gcgtgttctg caaacagctc gtcaggtcgc gcgcgtgttg cgcgtcgtcg
     7321 gccagcgcgt tgcgatcgca cgcctgcatg cagggttccg tgaccggatt gcagcgcgca
     7381 tagcaagtca cggtgtcctg acagctcgcg tactcgacgg gtttggcgtg gaagaagctt
     7441 ggctgcaaag gctgtttcgg ctggcagctg gccaggagca gcaagcagac gaagcgccac
     7501 atgaccaacc ctatctcgcg aaatacgctg ttcgtgcaag gatctgcatc acgaggtgac
     7561 cccttgcttg tccggctacc ggcctagccg atcacagcga tctccgtgga ttcatacgcg
     7621 ccagatctgg ctctgaagtt gccatctgtc catgcaccat gcggacttca gcggccaccc
     7681 gactagccac ctatgtactt gcggccgccg cgtgcttgcc cctcggggcg tgcagcgacg
     7741 agctcgagat cacgccgctc tacaacccgg cgaacggtcg cgtggtgatc cagacgaacc
     7801 aggacatcag cggcaagcag ctgtacgtcc aagttcgccg cggcaagttc ggcgtcctcg
     7861 actgcaagca gatggcagcg aacctgacgg cggttgaaga gctgtcgggc aatcgcatcg
     7921 acggtccgtt cgtcgacccg cagctgacga agcccttcta cggcggcccg gagtggatga
     7981 acccgaccgc gcagatgatc gcgcaggcgg aagccggtac cgactccatc attgatgtct
     8041 gcttgatgga cggcagcaag gtcgtcgtgc agatggagcg cgacttgttc cgcgcatggg
     8101 acgacgctcg caagcagggc atcggcggca aggccgacaa cccggagagc ggcgagcagt
     8161 acatcaacag ccccgtcgcg tacggcgagc gctgcgttgc agagctcggc gagattccgt
     8221 tcttcgagaa agaagccgac ggcaagtact cgacgtacga ctgcatcgag tcgaccgaga
     8281 tcccgatgac ggtcacgaag gccgacggct ccgtcgacaa gccgcgcgaa ggcacggtca
     8341 accagtgcga caacccgcag tacatctact cgctctgcga ggccggcccg cgcgtcgcgc
     8401 agcgcacgaa cgagcaaggc acgcgctggg tgctgctctg ccgcaagagc aagggcggct
     8461 acgcgtcgaa ccagtacaac gacatcgcga tgatcgggtc caacccgttc accggcaaga
     8521 cgtgcttctt ccagaacgcg ctctactcga agacggatgg cggtcacgtg ccgcaccccg
     8581 ccgacaagga gaagtcgcag aacctgtgga gcggcgtgca cggtggcctc ggctcgggca
     8641 tcgagtgctc gacctgccac gacgcggacc cgttcattca cacgccgtgg atcgatggcg
     8701 cgaaggatcc gcaaggccgc ccgatcgtcc cgaagatggg catcgacgcg gatctcgcga
     8761 tcggcgccaa cgacacgccg tactcgctca tcaatgcgaa gggccagggc tggaagtacg
     8821 agcgccagat cgtttcgccc gaggcgagcg cgtgcctcaa gtgccatcgc atgggcagcg
     8881 gccgctggac cgagtcgtgg atcagccgcc tcggtggcac cgacagctcg tggaccggca
     8941 tcacgaccgc ggcgttcaat cagtcggcgc acaagtactg gatgccgccc gatgtcgcga
     9001 tggataccga gacgatgtgg acgtcgtcgg tgttcaagac ggcgctcgac ttcatcgaga
     9061 gctgcggcaa gaaccagtcg aacccggcgt gcatctggaa ggacatcccg acgaagctcg
     9121 cgtcggatac cgccggcggt gcgctgcgca acaaggtgac gctggcggat gacgagctcg
     9181 cgaagcaagc gacgacgatc atcggcatga acaagaacac gccgtcgcag atctgcgccg
     9241 agtgtcacgc gccgaatcag acgacgctgc gcgactggca ggagaagacg gacaccgcga
     9301 tgtcgacgtg cctcaagaac gtcgatgacg gtggcgtggc gacgaccgcg aaggaagaca
     9361 acgtctcgat cggcaaggac gagttcaaga cgtttggtcc gtacgaagtc gcgctcggcg
     9421 gcaagatcga agtgaagatg gccggcaccg gcgacgcgga tctctacgtg aagcgcggcg
     9481 ccgagccgac cgttgattcg tatgactgcc gcccgttctc tcgcgagccg aacgagcagt
     9541 gcgtcggcac gcagttcaac gcgaacggcc cggcgaagtt ctgggtcggc gcgaatggct
     9601 ttgccgatag ctcgaagatc acgatcaccg tgacgtacga caagcccggc gcga
//
LOCUS       DATLBZ010000352        26921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9898633, whole genome shotgun sequence.
ACCESSION   DATLBZ010000352 DATLBZ010000000
VERSION     DATLBZ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 26921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26921
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9898633"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VMZ53_18150"
     CDS             complement(<1..552)
                     /locus_tag="VMZ53_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30440.1"
                     /translation="MRAQRKPHERERERERELARERDLERERDLEREPALEREPQREP
                     ELEREPERELEREPDREPELERELSGCTSSRTMSGWGGARANAGRPAKGAHVSEPHKT
                     RPALAARHPVHVRARFVRGVGVGRRLSRRRAYAALGRALSLSLARADFRIVHFAIVGS
                     RLELVAEATDQYALARGMQGFQVS"
     gene            885..1775
                     /locus_tag="VMZ53_18155"
     CDS             885..1775
                     /locus_tag="VMZ53_18155"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HUS30441.1"
                     /translation="MSDDRDGNVVETVSDRGDASTSLSEWMLERWKSEQSKSTPTDKP
                     PTRFRIGAAGRLQQGGRHDERDDAPDHASDHGGGGAGADRDYDYDVDDDSSDEPRPRA
                     RGSSPSLPVPMSNKSSSGDMAGASDAALVELMRAAASVDDVAADADGAIGDVPEVSRK
                     SRPRHRRRDRPRSKTIAMKRLTRESLRQGALANPPVEGVERPQTRSECAGAARPCPWV
                     ACKHHLYLDINPKTGSIKINFPDLEPWELEHTCALDVADNGGLTLEEIGLITNLTRER
                     VRQVEVRGLLTLKARSGPLR"
     gene            complement(1780..3018)
                     /locus_tag="VMZ53_18160"
     CDS             complement(1780..3018)
                     /locus_tag="VMZ53_18160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUS30442.1"
                     /translation="MPTICGTDLSPRSAAALEVACALAAHRGERDVIAVHVIEEEAQG
                     EVARQALDAHIATVNRGAVNIRAELVVGTPEDSLLSFAETEGADLIVISASSSGTSWF
                     RLGTTAEKVIAHTRVPVIVVRDPAPWLAFARREKPLRLLLGIDDSVACDIGIQWTHAL
                     RQRGDVEVILGAVYYPDDAAEHYGLDHGTAVDRHLEVEKLIMRDLVRKFGNSDKVDPR
                     AIRGLGRIGDHVIELAKEAQVDAILVGTHQRTGLGRLGSVSGVIIHDAPQSVVCVPPN
                     ATISTLAVPRLTSALVATDLSTFANRAVPFAFALTDEGGEVHIAHVVDNDAEIDEADV
                     KRQLLALAPTGMARQVSAYILRGEDPAKTIAQAAARLGADAICIASHGRSGITRALVG
                     SVADKLLRATRKPVLVLRPA"
     gene            complement(3018..3404)
                     /locus_tag="VMZ53_18165"
     CDS             complement(3018..3404)
                     /locus_tag="VMZ53_18165"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HUS30443.1"
                     /translation="MKAKGVTAEVPVVDPADRRQAPRVLVDLEVDYASEENYLFAYIT
                     DISETGIFVRTTSPEKAGTRLNLRFRPDDASPQIQVEGEVIWVNPYRPGAPDNLHPGM
                     GIRFVELDNELRDRLLELVRRFAYLT"
     gene            complement(3493..5853)
                     /locus_tag="VMZ53_18170"
     CDS             complement(3493..5853)
                     /locus_tag="VMZ53_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30444.1"
                     /translation="MEFLSRHLGRWHLALAWVFVLAALVLLPGLNSTGLWEPQERQLA
                     DRVAPRVELAKKVNQAIPPLEQVPPKDNCYRMAPKDAKARTLTNRAMTWGRDHVSDTD
                     AGRRLPLALLGLLTVLASAGIAMRFAGSRAGVLSTIILLSMPLLVLQSRMLTSEIGTA
                     AGAAMTLYGFIALGERRGQLGIIESLVAVVSLAAGVTVGFLAGGALLGVLVPVGAYAA
                     AHGLGFSLFVTLIGKAKEDRPPLVEQIKALVATLLAIGVAAILIYQLYSLVRPQPGLV
                     PPLRAVFGKAIVGSGCYSSALGGMWRPDDDLRYSFDSTFEQIAYGTFPWGILGPISMF
                     ALIISPERRKRLVGGVCLAWGAGAWIAGEAFQRKVGFTIYAGFPALAIATGVWIDSLL
                     PSRAVADDSAPPADDAKPRSLWPAGIILVGFFTALAMIDLGKDLQSFTERLTSLLVGS
                     DQIAYPKAARMIVPTRAWILVYGALVGVGFALAVAFWRDDAKHRTKRLVAKIGAITAV
                     SATLLFALFWSYGWQRSLSVHLSSKAMFDTYRDLRKNGDQLVIMGDMGDAPYDYAPDA
                     KPEVVTGRDQIVAALTRQNRVFAIAPQGELCQLHREIGGKDYFVLDDRNVRSLLLSNK
                     VAGSSDKNPLRTTILHEEPKTIPFKPKGRVVFDGRIELLGWSMPKKIGRGDKFDVKMF
                     YKILQPVGANWKILFHFDGPLRFNGDHEPINGRCATSTWQPGDFIVDTYRVTAGGGAF
                     APGAYEVWTGFFTGSNPNWKNMTVSEAPPEMRDTADRVKITTIVLD"
     gene            5889..6797
                     /locus_tag="VMZ53_18175"
     CDS             5889..6797
                     /locus_tag="VMZ53_18175"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS30445.1"
                     /translation="MSSARAGELAKLHRDVPVARYKSLVGHWISRWFRPPRPAKHDAP
                     VKAHAGEVVITFGGHATVIARYDKLGIAFDPMLGRWVGGVRRAVEPGLAPGDFEDVGL
                     ILISHRHADHLHLPTLQKLPRKATTIVVPAGGAAWVSALGFARVVELAPGADLELRGI
                     QVIGYATSHGDGELARGLSYVVRPVPGGPITGPSLYLCGDSGYFSGFADIGERFAPDI
                     ALLPIGGFYPASFRSRHMSPLDALYAFEDLRARLLVPIHHGAFALSYEKLDEPARWLT
                     ELATSRGLRDHVNVMTAGQTQRFPAL"
     gene            6810..7574
                     /locus_tag="VMZ53_18180"
     CDS             6810..7574
                     /locus_tag="VMZ53_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUS30446.1"
                     /translation="MSPDVSIVIPVYNEEGILREAVMELLEGLDTVRLALDAPDLRFE
                     IIIAENGSRDHTVELAEHLAAESAAGELSPDRRASVRTFSLGEPNYGKALRRGILEAR
                     GTWVICEEIDLCDRDFHRRALSHLQHGDADMVVGSKAMKGSNDHRPLFRRAATRVLNG
                     MLRATLDFRGTDTHGLKAFHRETLLPTVERCVIDRDLFASELVIRAGREGVRTVEIPI
                     RLDEKRPPAINLVKRVPNVLRGMAKLTYVIRFGGKP"
     gene            7577..8659
                     /locus_tag="VMZ53_18185"
     CDS             7577..8659
                     /locus_tag="VMZ53_18185"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUS30447.1"
                     /translation="MRAPLKHCAVSIDLDGIACYYRIHGLGRSPAELEHVILERALPR
                     AAALFAQRGLHVTWFIIGRDADGASTLPDRARRAANAQRLRTLADQGDELANHSYSHP
                     YDLARLAPATIDDEIASCDRVLRDILGIRPAKPATAPDLVQLRTRKDSSDERVTSVEA
                     NPAPMTAPRGFRAPGYDVAPAMLDSLARLGYRYDSSIFPAPGYYAAKAVVMSALALAG
                     RPSGAVLTNPRALAAPADPYRPSMKSPWRRGQAPIVELPIAVTPYARVPAIGTTLLVA
                     PPWARERMLAWMQSRAFFNLELHGIDFADADKDGIPGELVDRQPDLRIPIDDKLATLD
                     RLLDAIADRWQFATLAEVAADVQRDE"
     gene            8986..9363
                     /locus_tag="VMZ53_18190"
     CDS             8986..9363
                     /locus_tag="VMZ53_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30448.1"
                     /translation="MKEWMGMGSETMTVECKAGVAMMSLAVDEPRGDGGEKWSRAMSN
                     DAWMTLWMSLESARWRELAAACPDTGDPNVGTGITDLELELSDGTTTKKLECSAMRLS
                     SQHGRVIDAFEKAAEIVDLVPST"
     gene            complement(9365..9826)
                     /locus_tag="VMZ53_18195"
     CDS             complement(9365..9826)
                     /locus_tag="VMZ53_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30449.1"
                     /translation="MKHAVIVAALLFAGCGKKSEPSGPSCADAVDKAINSMPGGPGGG
                     GADIKDKLRAIYTKHCTDDKWNADAIKCFATDAHDMPSMKACRAKLTADQQEKVMGEV
                     RTVMMGAMGGAGPMHGAAAPPAGDTPPAGAGPAGGGPTPTGSPAPAGSAAP"
     gene            complement(9847..10596)
                     /locus_tag="VMZ53_18200"
     CDS             complement(9847..10596)
                     /locus_tag="VMZ53_18200"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HUS30450.1"
                     /translation="MPWLKHRLRDADVWAKVDEGGALVTDRDGRVEIVYKPADGARVY
                     RAGARNLAKVAAAAPVEIVVGEPAPEKSAAAPANGAVAKTPTRNAGAAPRGKALVTEE
                     NATAHALAEIAKAPANAIHVWTDGACSGNPGPAGIGAVIVDAGKETELSEYLGDGTNI
                     VAELTAILRALETIEDRTRPVIVYTDSQFSIDMLTKGWKAKKNIELVAEVRQTAREFK
                     DLRFVWVRGHANVPLNMRCDELARQAVARRR"
     gene            10650..11687
                     /locus_tag="VMZ53_18205"
     CDS             10650..11687
                     /locus_tag="VMZ53_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30451.1"
                     /translation="MVRIRVGLLVAVAALGLGVGGCKKNDAKKDETAAGKATTGSATT
                     TATPTGGGALVAPAGSDDLSLLPADSEMVLGLNFAQLQQSALWKQFSPKLMEKMEKGL
                     NDFKTACGFDPMAAVKTVSMGLKGIGADQPDGTIVVHGLDKAKSMACLDKAKTEAAKQ
                     GSEITVDGEVFTVKDKNGQLTAWTFVNADTLVGAVGPTASKDTVTAMAKGTSALKSSQ
                     TFLEMYNKVNTKESLWLFVNGNAPFMAKAAQAGVKPKAVFGSVNVTDGLTVDMNIRLG
                     SADEATQLVNMMKGQTQSPQVKQMFDKLDVTAVGTDAKIAVAMSNAKLQQLIGMVGGM
                     MGGLGGGAGGP"
     gene            11706..12743
                     /locus_tag="VMZ53_18210"
     CDS             11706..12743
                     /locus_tag="VMZ53_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30452.1"
                     /translation="MKRAITTTIAACCLAATLVVAAGCGSKKSGKSPEVTGLAAVPAS
                     AQVVIVTDVARVLSSPIIERAIDQLMLRDADLAARWQKLRDTCKIDMATISHVALAIG
                     PKTGDSPGTGPVLMVVTGKLVEADLATCVRAMVGQGGGQLIAKEAYGHTLYQAVDANR
                     TMYFAFGRPDTVVLGSNEKYVTEALGTGTKVSDDPEMKRWISRADQKAPVWAAGRVDD
                     RVKTGLVRVTNGQLKEGPQAMLVALDPTNGAKIEVSSVMASDADAKTLESYAKTQLGA
                     LAMAAQAKRLGRVVEKVQISSENETVRFKADLGMDEVNLLISVLDGEAPREQISPPPA
                     SGSAIGSGSGL"
     gene            12752..13195
                     /locus_tag="VMZ53_18215"
     CDS             12752..13195
                     /locus_tag="VMZ53_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30453.1"
                     /translation="MSSKRTESKKKINTKKAPAKKTAVKKAPKEKVEAKPMGPRHPKA
                     RVAGLHEGKEALAKTLAASLATETEDSDSIASRLKTASNMQLLRLQKVVATVKDKWGS
                     REKLIAALGEQAKASKDKDFLAKLSTFSLPRLLDMAQSATRRAGA"
     gene            13225..14946
                     /locus_tag="VMZ53_18220"
     CDS             13225..14946
                     /locus_tag="VMZ53_18220"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUS30454.1"
                     /translation="MRCTSLLLVIALARVAAAESDDEIERGAPEVPAGESLVRADQAA
                     WRYQLITAPALAPQVGALALSGLDVVAGRAQQPIDVLGGGPTVPAAWPYGLDTVTRAT
                     GTLEPTPPAQMRIAAMYAITTFTLAPEDRGLRVLEIRVRYHDGCSVYLNGIEVARRSL
                     ADNASPVALAQRPHGPEWETFYVPAAPHLLRLGENVLAIEVHPSGKRDAPDLAADVVA
                     RRELGVVRGPVLADVTATSATINVETDASIDAVLEWGTGTTLDHKVSSTVGRLHAFTL
                     TNLPPKTKLSYRVIAGAATTATYAFHTAPGAGDVIRIGVYGDVRGGHDTHRRLVEAML
                     GEGIDLVAVTGDMVLRGTDDADWQKFFAVTRELLAQIHYVPAIGNHDVGLTNGEANVF
                     TLPPAPPGRPERTYWYSMELADIHLVFLDSNAYDRSEQEQWLDADLAAARGKNVRAIL
                     AFTHDGPYSRGTHRGNETARDRFVPILAKYHVDLVVAGHDHLYQRGDAGGIRYIVSGG
                     GGAGLYQVSCGVSGKPKCPDDGMQKVLVEHHYLVLAITNDTLEMCPRKADGRLLEKCV
                     RYKLWRP"
     gene            14959..15879
                     /locus_tag="VMZ53_18225"
     CDS             14959..15879
                     /locus_tag="VMZ53_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30455.1"
                     /translation="MRALLLLVAVAGCYREATPASAPPPANKQQPLVAPRDVDDELSF
                     LPKESELVFGFDMTTLRTSAAWREQIEPALAASSALEKTRKLCGFDPLASITHLSIGA
                     RKADAGNELVFTLAGGDAQQQIGCLTKQLDGDYAAQPAGDVTLITKKQDTFALALAPV
                     GRQHILGVSAPGVDAKRVRAQAAIGSPLRASPAFMNLYDKLERGASVWFVLNGASPMF
                     QSFSLGVRPRYIDGTIVITDRYVWTTRITMETPADASQLATMFRGAANQVKQMVETLE
                     IREDGEVLHVDVVLTQPQVQTILALLGTMI"
     gene            15928..16803
                     /locus_tag="VMZ53_18230"
     CDS             15928..16803
                     /locus_tag="VMZ53_18230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS30456.1"
                     /translation="MEQQARTVDGDSLSYRVWGRAGATPLMTVHGLVSSTHHWLDFNP
                     YYAAHRQVISWEYRGHGGNPAPHNARAISIAQFADDAYQVWRAAGSEQPVVVVGLSFG
                     VQVALELYRRHPDCVRALILICGTAGHPLDRVSESASLRRNLTALLRTLSSQRIVSTP
                     LLGFLRTRLGTRIAREITYLSGGANRDSCPPAMLQGLFKHVGSLDPELIGAVMAAYLE
                     HDAHDVLHTITVPTLIVAGDRDQLTPLAVAERMRDTIPGSELVVFPGHSHLVQVEKPR
                     EVHAAIDAFLAKHQL"
     gene            16865..18271
                     /locus_tag="VMZ53_18235"
     CDS             16865..18271
                     /locus_tag="VMZ53_18235"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HUS30457.1"
                     /translation="MKSAELARLDATAQAELVAKGEVSASELLDAAEQRVQRLEPLLH
                     TMAAVDFARARAARPAKGPFHGVPFLLKDTTPYPGLRWAFGTRLMRNNMASPPTPFAS
                     RVDAAGFVVIGKSTMSELGLLGSTETLAEGITHNPWDLSRSATGSSGGSAAAVAAGLV
                     PIAHANDGGGSIRIPASANGVFGFKPSRGRTVTAIVGRSDFGDITSDGCVSRSVRDTA
                     RFLSLVEDDVSGYRMGFVAGPEKKRLRIGTWTQTFDGREPDAEVLRAHAEAVELVKEL
                     GHHVEEATPPRFETEVLGDAFFLVAGAAVAGLVQMVGGMRGKIVRDDELEPFSWALAR
                     RAMERGPSALEAAREEFARAARAHLDATASYDVVLTPVLATKTWHIGHLSPLVPVDEL
                     IRRTAETVAYTPIHNIAGAPAMSVPLHTTSDGLPLGAHFAAKPGDDAMLLALAYELES
                     ARPWADRWPGISAPMLMA"
     gene            18273..18881
                     /locus_tag="VMZ53_18240"
     CDS             18273..18881
                     /locus_tag="VMZ53_18240"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS30458.1"
                     /translation="MPQVLKDEVRTAIVDAALAVFARDGFEAATMASIAKAAGVGAAS
                     IYRYYESKDELFDAVITPELTKRFESVLDRRVSALARNTLRGEAVDDAAGEMLQFWIE
                     NRLAVVILLDRAGGTRYAHYGERFVELLVKHTIAELRAAHRNVEISPRGRFVITQLFA
                     NTRRLLASVLAEYSRPDELREAIETFWSYQVAGLRGLRAHLR"
     gene            complement(18883..19647)
                     /locus_tag="VMZ53_18245"
     CDS             complement(18883..19647)
                     /locus_tag="VMZ53_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30459.1"
                     /translation="MRGLVLVALVAACGGREAPATLSSRADPPRPSAVADQDGDEIVD
                     AKDGCPTKPEDFDLRADTDGCPDLDDDRDGVPDATDTCFDTPGLAALHGCPDEDCVFI
                     TDITDCWLSPIAFDQSPPRAFDHVVAVIEKYPEIREVTLQTQQTLAQPAGTARARLER
                     VKKELVARGVPADLLVLKESEPADPGEKPRSDVFGLVTKQRFEDGKFRETVCADELGP
                     VRRVARAKNYSCKPSVCGNGRCGVHEDVTNCPQDCP"
     gene            complement(19647..19997)
                     /locus_tag="VMZ53_18250"
     CDS             complement(19647..19997)
                     /locus_tag="VMZ53_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30460.1"
                     /translation="MQPAYVEVDLGFFPLMWILYLVSPWLAVNGHAQKQSWGKVQLTL
                     PPGRHVFEAWYPYLFSSQQSKGTIIVDLMPGGSYKLKYRPAWIVFLGGSFTMVGQPML
                     PQAHVHQFPPPPHQ"
     gene            20068..20511
                     /locus_tag="VMZ53_18255"
     CDS             20068..20511
                     /locus_tag="VMZ53_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30461.1"
                     /translation="MAFGCRVHFDERRDAGGPVADAAAPCVAGECSPSTTFRVTCSTG
                     PKSADEICGSMGFGTTAVVRGYYWFQCGGTLDRCPGGWDGIDCPAWCPGGTDCVGVSL
                     CGSAGESLVTEKTGDGSTTFDPMETGRTCMGYNPGWTVRIRCYLR"
     gene            complement(20524..21666)
                     /locus_tag="VMZ53_18260"
     CDS             complement(20524..21666)
                     /locus_tag="VMZ53_18260"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUS30462.1"
                     /translation="MSKWTVEELTPGRTVEAIFWPNTAEGSQFKLRATHLDGRRAPKV
                     VLSNDDRIVAGVPCLVRVLEIHKPERDDRGRIEVEFVSRAPFRIEGMYLDPVVSKKLQ
                     VLLTSGLNILLDGPQGCGKTVLARSIADTLGMVFVFFNCGAVVEASDFFVSIQVRASE
                     SGAPVTDFVKTDILVALEEAAEHPDRKYLVFLDELNRCQESARNALMPALDSTRRVFH
                     PIENTFIPIPENVQFIAAVNRGREFTGTFGIDAAQLDRFAPLQMDYPPPKAEVEILAR
                     RHPEVSASNLEIIVAVADAIRTAADLPGSLSVRATDEACVYLKHPLFADDGKRALPEI
                     MKTSFCARFPGRWDDITTDAGAVWGIVRKTLATLKVPLPGDEKAAT"
     gene            complement(21674..26665)
                     /locus_tag="VMZ53_18265"
     CDS             complement(21674..26665)
                     /locus_tag="VMZ53_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HUS30463.1"
                     /translation="MTDLAIYRRLASGEVGSADLEEIASWPLARRVAYLGALEPLVLS
                     GDAAVREKALAALAGARGYEGVRAIVGRLDDPEESVWRAALEALRAVARDVPERYAHA
                     LFHSRPEVRRAALEGELSLSLGKLAIYLRADPATADLASKLRWPDDALPVALELYEAG
                     HVPASELVEALTRASPTELRELYKREVGRGADIVDAYLERGVIAEPMGRDVLDVVVDA
                     LAQRTVPRAIDTFVECVAPNKAIGGVLGRRAAVALLRYWARTERPEPLLVSACVALEP
                     RVIEFAGFDARLAFEATRGLFRYRWPVKPTSLQIMRLLASDVVRGDLALAAAVAGLLP
                     AKRLDKLREKLGQAEIVARLVADDHGWEQILRLPGEVPALETSWLAAVEKQDYKRYIQ
                     LAGRALGVLTHKRLERFVQLLPRRHRQPAFEAALALYADAGGETLEVVCKTVAANVDR
                     TTAASLIQGLSATNVLHALLRALPARDLFAAAELLVEPLARRIVEIVSEEPLPRDQEL
                     ALVAAFARHKDAAIIKWRTDVTAEAPETPLAPQVTAKRDLTAAERERIARCNDRDLEA
                     ALAPARTGAVGGLVAAVSGRDAGPSIPICVALLGCADPLVDVAKALDRFSEATSKFDA
                     ALDDGECTWRRVPTADLPPLALARLWRWEQHLFAFLDWLHREPSVVAVLARAEALPGR
                     VARDTLWRAVSEALMLVRYRDVERFRSYATEELAKCCAVRIDRDIGRHAARILVALVE
                     SRIIPVESVRDAVLDRVADADVATREYVGRFVRLVGVAAAPPPAPPPPAVVLDAVRAC
                     TDIQRLAQWCRDPRPQVVQEAALALVVLGEKGEIKLAELLRDAAAFRNDVGEPAPEAV
                     LPAPTPLLSSILLWEHEPAIVMVREVLASSLPAAWQFYIQLSLHARGEPGALEHALAA
                     VRATPGTWYFRRDDWEALVRVADLVTCSVALADAPHHHAYQRAIQVLLALTRPTDDVH
                     AALVRFLEASGDRPLHLRVAVARRLAELDDTTGLPLLVEYIADERAQDWMFTLELVPA
                     RALDGVARTIVDAALIGGHTVCSERRMWELVQRLRDRSRLSARVLAELDMRILEHAST
                     PAARRAAAKFAVAFETGDDRLRRVADVFAWGVRRGVELAGKLFAFHLTAKETDFGHTK
                     LDGSRIFVTALPMLRGETHGRDIVEGLVLHEIGHHIYHASPEAQKIWKQAHDEGLGHF
                     LNLIADEHLERNLRAIDPDYGNRLKRLGAYAFQHAPQEIKLAALLASLRGFAAPALIA
                     ADLEVAFDEQSVRMRRGAILGELDRNGHPVARFSRALRMGLGNRANDPLVAKALALCG
                     KDLRTLDMAGLYALTKELATLFGGATRLAQVFGGPEGLVFGERDDDVFGAGIDDEILQ
                     REVERILDPRRSKSKRSDKGGPPRLQLNVSPDQHFDKITRVMRVRGDQEVHRNLATNV
                     SRHAARLRQHLDELGLRWEPQRARTQGRALDRTRLRALVTRNDPRILIARNPIRRTDL
                     FLGVIIDCSGSMQAGNNLDRARRFAILVAEAVARLPGVEARFFGFTDSVIYDAGTATD
                     CNVVALQSEGGNNDAAALYHAANVALNSGKRAKVLVMISDGLPTECSVAALKGLVVTL
                     TRRKNIVCAQVAVRKLDEVCFPNYLVLDDSELDIAVAKFGRMIGDLARRSLAS"
BASE COUNT         4525 a         8865 c         9224 g         4307 t
ORIGIN      
        1 ggagacctgg aagccctgca tgccgcgggc gagcgcgtac tggtcggtgg cttcggcgac
       61 gagctcgagg cggctaccga cgatcgcgaa gtgcacgatg cgaaagtcgg cgcgcgcgag
      121 cgacagagag agcgcgcgac caagcgcggc gtaggcgcgg cgtcgcgaga ggcggcgacc
      181 cacgccaaca ccgcgcacga agcgcgcacg gacgtgaacg gggtggcgcg cggcgagggc
      241 ggggcgcgtc ttgtgcggct cgctgacgtg cgcgcctttg gcggggcgac ccgcgttcgc
      301 gcgtgctccg ccccagccgc tcatcgtgcg actcgacgtg cagcctgaga gctcgcgttc
      361 gagctcgggt tcgcggtcgg gctcgcgttc gagctcgcgt tcaggctcgc gttcgagctc
      421 gggctcgcgt tgaggctcgc gttcgagcgc gggttcgcgt tcgaggtcgc gttcgcgttc
      481 gaggtcgcgt tcgcgtgcga gctcgcgttc gcgttcgcgt tcgcgttcgt gcggtttgcg
      541 ctgtgcgcgc atgtgcgatc gcgcgcgcgt ttgtttcgcg cgtgtttctc gcgcagcggt
      601 gagagcggcg atgtgcgcgc cgatttccgc gtggagtgcg cgaattccat cgagcgcgat
      661 ccgtacacct gaaatatgaa aacgccgaga tctctccgtg acaaatttga ggagagaaga
      721 cttgcgaatt tcgtttatag aagtttaggg tgtgaatcgc gggaacctgg gactgatctg
      781 atcggcacgc ggtgggtgag agcgtagggc tctgggattc ggagcccgct cagccgcgtg
      841 ctcgtgcgcg agcacgatca gtcgcgggcc gcgaggagct agtcatgtcc gacgaccgtg
      901 atgggaatgt ggtggagacc gtcagcgacc gtggtgacgc ttcgacgagc ttgagcgaat
      961 ggatgctgga gcgctggaag agcgagcaga gcaagagcac gccgacggac aagccgccga
     1021 cacgctttcg catcggcgcg gcgggccggc tgcagcaggg cgggcggcac gacgagcgcg
     1081 acgacgcgcc cgaccatgcg agtgaccacg gcggcggcgg cgcgggcgcg gaccgtgact
     1141 acgactacga cgtggacgac gacagcagcg atgagcctcg gccgcgagcg cgtggcagct
     1201 cgccgtcgtt gcccgtgccg atgtcgaaca agtcgtccag cggcgatatg gccggcgcgt
     1261 ccgatgccgc actcgtcgag ctcatgcgtg cagcggcgag cgtggacgac gtggccgcgg
     1321 acgcggacgg cgcgatcggc gacgtgccgg aagtgagccg caagtcgcga cctcggcatc
     1381 gccgtcgcga tcggccgcgc agcaagacga tcgcgatgaa gcgcctgacg cgcgagtcac
     1441 tgcgccaggg tgcacttgcg aatccgccgg tcgagggcgt ggagcggccg cagacgcggt
     1501 cggagtgcgc gggggcagcg cgcccgtgcc cgtgggtcgc gtgcaagcat cacctctatc
     1561 tcgacatcaa tccgaagacc ggatcgatca agatcaactt tcccgacctc gagccgtggg
     1621 agctcgagca cacgtgcgcg ctcgacgttg cggataacgg cgggctgacg ctcgaagaga
     1681 tcgggctcat tacgaacctg acgcgcgagc gcgtgcggca ggtggaagtc cgaggcctgc
     1741 tgacgttgaa ggcgcgctcg ggaccgctgc gctgaggcct taggccgggc gcagcacgag
     1801 cacgggcttg cgcgtcgcgc ggaggagctt gtctgcgacc gagccgacga gggcgcgggt
     1861 gatgcccgag cgaccgtgtg atgcgatgca gatggcgtcg gcgccgaggc gcgcggccgc
     1921 ttgcgcgatc gtcttggcgg ggtcttcgcc gcgaaggatg tacgccgaga cttggcgagc
     1981 catgcccgtc ggcgcgagcg cgaggagctg gcgcttgacg tcggcttcgt cgatctcggc
     2041 atcgttgtcg acgacgtgag cgatgtggac ttcgccgcct tcgtcggtga gcgcgaacgc
     2101 gaatgggacc gcgcgattgg cgaatgtcga gagatcggtc gcgacgagcg cgctggtcag
     2161 gcgcgggacg gcgagcgtcg agatggtcgc gttgggcggg acgcagacga cggattgcgg
     2221 cgcgtcgtga atgatgacgc cggagacgga accgagacga ccgagccccg tgcgctggtg
     2281 cgtgccgacg aggatcgcgt cgacttgggc ttctttggcg agctcgatga cgtggtcacc
     2341 gatgcggccg aggccgcgaa tcgcgcgcgg atcgactttg tctgaattgc cgaacttgcg
     2401 gacgaggtca cgcatgatga gcttctcgac ctcgagatgg cggtcgaccg ccgtgccgtg
     2461 atcgaggccg tagtgctcgg ctgcgtcgtc ggggtagtag accgcaccga ggatgacttc
     2521 gacgtcgccg cgctggcgca gcgcgtgtgt ccactggatg ccgatgtcac aggcgacgga
     2581 gtcgtcgatg ccgaggagga ggcgcagcgg cttctcgcga cgcgcgaacg cgagccacgg
     2641 cgctggatcg cgcacgacga tcactgggac gcgtgtgtgg gcgatgactt tctcggcggt
     2701 ggtgccgagc cggaaccacg atgtgcccga gctcgaggcg gagatgacga tgaggtccgc
     2761 gccttcggtt tcggcgaacg agaggagcga gtcttccggc gtgccgacga cgagctcggc
     2821 gcggatgttg acggcgccgc ggttgaccgt tgcgatgtga gcgtcgagcg cttgccgcgc
     2881 gacctcgcct tgcgcctctt cttcgatcac gtggacggcg atgacgtcgc gctcaccgcg
     2941 atgggcggcg agtgcgcatg cgacttcgag cgcagcggcg ctgcgaggcg aaagatcggt
     3001 accgcagatt gtaggcatta ggtcaggtaa gcgaagcgac gcacgagctc gagcaggcgg
     3061 tcgcgaagct cgttatcgag ctcgacgaag cggatgccca tcccgggatg caagttgtcg
     3121 ggcgcgcctg gacgatatgg gttcacccag atgacctcgc cctcgacttg gatctgcggg
     3181 ctcgcgtcgt ccgggcggaa gcggaggttc aggcgcgtgc cggctttctc gggcgatgtg
     3241 gtccgcacga agatgccggt ctcgctgatg tctgtgatgt acgcgaagag gtagttctcc
     3301 tcgcttgcgt agtcgacctc gagatcgacg agcacgcgtg gggcttggcg acggtcggcg
     3361 ggatcgacga ccggcacttc cgcggtaacc cccttggcct tcaccagggc gtcataccgc
     3421 tggcttgcga gcggcgtcaa tgacatccga tgaccctgca tgaaccgcga ccgaggagcg
     3481 accgaccgtg tgtcagtcga gcacgatcgt ggtgatcttc acgcggtccg ccgtgtcacg
     3541 catctcgggc ggcgcctcgg agactgtcat atttttccag ttcggattcg agcccgtgaa
     3601 gaagcccgtc cagacttcgt aggcacctgg cgcaaacgcg ccgccgcctg cagtcacgcg
     3661 gtaagtatcg acgatgaagt cgcccggctg ccacgtcgag gtggcacaac ggccgttgat
     3721 cggctcgtgg tcgccgttga agcgcagtgg gccgtcgaag tggaagagaa tcttccagtt
     3781 cgcgccgacg ggctggagga tcttgtagaa catcttcacg tcgaacttgt cgccgcggcc
     3841 gatcttcttc ggcatcgacc agccgagcag ctcgatgcgg ccatcgaaca cgacgcggcc
     3901 ttttggcttg aacgggatgg tcttcggttc ctcgtgcagg attgtcgtac ggagcgggtt
     3961 cttgtccgag ctgccggcga ctttgttcga gagcaggagc gagcgcacgt tgcgatcgtc
     4021 gagcacgaag taatccttgc cgccgatctc gcgatgcagc tggcagagct cgccctgcgg
     4081 cgcgatcgca aacacgcggt tctggcgcgt gagcgcagcg acgatctgat cgcgaccggt
     4141 cacgacctcg ggcttggcat caggcgcgta gtcatagggc gcgtcgccca tgtcgcccat
     4201 gatgacgagc tgatcgccgt tcttgcggag gtcgcgatac gtgtcgaaca tcgccttcga
     4261 cgacaggtgt acggagagcg agcgttgcca gccgtacgac cagaacagcg cgaacagcag
     4321 cgttgctgac accgccgtga tcgcgccgat cttcgcgacg agccgcttcg tgcggtgctt
     4381 cgcatcgtca cgccagaacg cgacggcgag cgcaaagccg acgccgacga gcgcgccgta
     4441 gaccaagatc cacgcacgcg tcgggacaat catgcgcgcg gccttcggat acgcgatctg
     4501 atcgctgccg accagcaacg acgtgagccg ctcggtgaac gactgcagat ccttgccgag
     4561 gtcgatcatc gcgagcgcgg tgaagaagcc gacgaggatg atgccggccg gccacagcga
     4621 gcgcggcttg gcgtcgtcgg cggggggcgc gctatcgtcg gcgacggcgc ggctcggaag
     4681 cagtgaatcg atccacacgc ccgttgcgat cgcgagggca gggaagcccg cgtagatcgt
     4741 gaaaccgact ttgcgctgga atgcttcacc ggcaatccac gcgcccgcac cccacgcaag
     4801 gcagacaccg ccgacgaggc gcttgcggcg ctccggcgag atgatcagcg cgaacatcga
     4861 gatcgggccc aagatgcccc acgggaacgt gccgtacgcg atctgttcga acgtcgagtc
     4921 gaacgagtag cggagatcgt cgtcgggacg ccacatcccg ccgagcgccg acgagtagca
     4981 gccgcttcct acgatcgctt tgccgaacac cgcgcgcagc ggcggaacga ggccgggctg
     5041 cggccggacg agcgaataca gctgatagat gaggatcgca gcgacgccga tcgcaaggag
     5101 cgtcgcgacg agcgccttga tctgctcgac gagcggcggc cgatcctctt tcgccttgcc
     5161 gatcagcgtg acgaacaacg agaagccgag cccatgcgcc gctgcgtacg ctccgacggg
     5221 gacgagcacg ccgagcagcg caccaccggc gaggaagccg acggtgacac cggcggcgag
     5281 agataccacc gcgacgaggg actcgatgat gccgagctga cctcgacgtt caccgagcgc
     5341 gatgaacccg tagagcgtca tcgcggcgcc cgcggctgtt ccgatctccg acgtcagcat
     5401 gcgcgactgc aagacgagca gcggcatcga cagcaagatg atcgtcgaga ggaccccggc
     5461 gcgggaaccg gcgaaccgca tcgcaatgcc tgcagaagcg agcaccgtga gcaggccgag
     5521 cagcgcgagc ggcaagcgcc ggccagcgtc ggtgtccgag acgtgatcgc gtccccacgt
     5581 catcgcgcga ttcgtcagcg tgcgcgcctt ggcgtccttc ggcgccattc gatagcagtt
     5641 gtctttcggc ggtacctgct cgaggggcgg tatcgcttga ttcaccttct tcgcgagctc
     5701 gaccctcggc gcgacgcggt cggcgagctg gcgctcttgc ggctcccaca gccccgttga
     5761 attgaggccg ggcagcagca ccagcgcggc cagcacgaac acccacgcga gcgcgaggtg
     5821 ccagcggccg aggtggcggg acagaaactc cacgacgcga acctatcatt gttaggatcg
     5881 cgggcatcgt gtcgagtgcg cgtgccggcg agctggccaa gctccatcgc gacgtcccgg
     5941 tggcgcgcta caagagcctc gtcggccatt ggatctcgcg gtggtttcgg ccgccccgac
     6001 ccgcgaagca cgacgcgccc gtgaaggccc acgccggaga ggtcgtgatc acatttggcg
     6061 gccatgcgac cgtgatcgct cgctacgaca agctcgggat cgcgttcgat cccatgctcg
     6121 ggcgctgggt cggcggcgtc cggcgcgcgg tcgagccggg cctcgctccc ggcgacttcg
     6181 aggacgtcgg cctcattctc atctcgcatc gccacgccga tcacctgcac ctgccgacgc
     6241 tgcagaagct gcctcgcaag gcgacgacga tcgtcgtgcc cgctggaggc gccgcgtggg
     6301 tgtccgcgct cgggttcgcg cgtgtcgtcg agctcgcgcc cggtgccgac ctcgagctgc
     6361 gcggcattca agtgatcggg tatgcgacga gccacggcga cggcgagctc gcgcgcggcc
     6421 tctcgtatgt cgtgcgcccc gtgccgggtg gtccgatcac tggcccgtcg ctctacctct
     6481 gcggcgacag cggctacttc tcgggcttcg ccgatatcgg cgagcggttc gcgcccgaca
     6541 tcgcgctgct gccgatcggc ggcttctacc cggcgtcgtt tcggtcgcgg cacatgtcgc
     6601 cgctcgacgc gctgtacgcg ttcgaagatc tgcgcgcccg cctgctggtt cccattcacc
     6661 acggcgcctt cgcgctgtcg tacgagaaac tcgacgagcc cgcgcgctgg ctgaccgagc
     6721 tcgccacctc gcgcggcctg cgcgaccacg taaacgtgat gacggcgggg cagacacagc
     6781 ggtttcccgc gctataaagg cgagccgtga tgtcgcccga cgtctcgatc gtcattcccg
     6841 tctacaacga ggaagggatc ctgcgcgagg ccgtcatgga gctgctcgag gggctcgata
     6901 cggtgaggct cgcgctcgac gcgcccgacc tccgcttcga gatcatcatc gcggagaacg
     6961 gctcgcgcga tcacacggtc gagctggccg agcacctggc ggccgaatcc gccgccggcg
     7021 agctctcgcc cgatcgccgc gccagcgtgc gcacgttctc gctcggcgag ccgaactacg
     7081 gcaaggcgct ccgtcgcggc atcctcgaag cgcgaggcac gtgggtcatc tgcgaagaga
     7141 tcgatctctg cgatcgcgat ttccatcgcc gcgcgctgag ccacctccag cacggtgatg
     7201 ccgacatggt cgtcggctcg aaggcgatga aggggtcgaa cgatcaccgg ccgctgtttc
     7261 gccgcgccgc cacgcgcgtg ctcaacggca tgctgcgcgc cacgctcgac ttccgtggca
     7321 ccgacacgca cggcctcaaa gcctttcatc gcgagacgct gctgcccacc gtcgagcgct
     7381 gcgtgatcga tcgcgatctg ttcgcctccg agctcgtgat ccgcgccggt cgcgaaggtg
     7441 tccgcacggt cgagatcccg atccggctcg acgagaaacg cccgcccgcg atcaacctcg
     7501 tcaaacgcgt gccgaacgtg ctgcgcggca tggcgaagct cacctacgtg atccgcttcg
     7561 gcggcaaacc gtagcgatgc gcgcgccgct gaagcactgc gctgtctcga tcgatctcga
     7621 cggcatcgct tgctactacc ggatccacgg cctcggccgt tcgcccgcgg agctcgagca
     7681 cgtgatcctc gagcgcgctc tcccgcgtgc cgctgcgctg ttcgcacaac gcggcctcca
     7741 cgtgacgtgg ttcatcatcg gtcgcgacgc cgatggcgca tccacgctcc ccgatcgcgc
     7801 gcgccgcgcg gccaacgcac aacgcctgcg cacgctcgca gatcagggcg acgagctcgc
     7861 caaccacagc tactcgcatc cgtacgatct cgcgcgcctc gcgccggcga cgatcgacga
     7921 cgaaatcgcg tcctgcgatc gcgtgctgcg cgacatcctc ggcatacgcc cggcaaagcc
     7981 ggcgacggct cccgatctcg tgcagctgcg cacacgcaag gactcgtccg acgagcgcgt
     8041 cacatccgtc gaggccaacc cggcacccat gaccgcgccg cgcggctttc gcgcgcctgg
     8101 ctacgacgtc gcgccggcga tgctcgattc gctcgcgcgc ctcggctacc gctacgactc
     8161 gtcgatcttt ccagcgcccg gctactacgc cgccaaagcc gtcgtgatgt ccgcgctcgc
     8221 gctcgccggt cgcccgagtg gcgccgtgct gacgaaccca cgcgcgctcg ccgcgcctgc
     8281 ggatccatat cgcccgtcga tgaagtcgcc gtggcgccgg ggccaagcgc cgatcgtcga
     8341 gctgccgatc gccgtcacgc cgtacgcgcg cgtccccgcc atcgggacga cgctgctcgt
     8401 cgctccgcca tgggcgcgcg aacgcatgct cgcgtggatg cagagtcgcg cgttcttcaa
     8461 tctcgagctg cacggcatcg acttcgccga cgcggacaag gacggcattc ccggcgagct
     8521 cgtggatcgg cagcccgatc tacgcatccc gatcgacgac aagctcgcaa cgctcgatcg
     8581 cctactcgac gcgatcgcgg accgttggca attcgcgacg ctcgccgaag tcgccgccga
     8641 cgttcagcgc gacgagtagg ctggccgtca tgggcagctg gcgcgatcgc aatgccggat
     8701 ggcgccggtg acgcctcgaa ggtgattgtt gatccatgcg cgcgcgcttc aaatgcgcac
     8761 atgcgtagct tcctctgcat cgtcgttgtc ctcgtagcct gcgatagctc ttcgaaacca
     8821 tcgtcggcgt tggcgaagcc cagcggcctc gaaaatcggc cctcagcaag cacgctccag
     8881 aaccttggac aaccgccgga gctacaggcc gcagcagcgg tggtcagcaa gctggatgca
     8941 ccaagcgaca cgtcggggcg atgtcgcgac agctggcgcg cttccatgaa ggagtggatg
     9001 ggcatgggat cggagacgat gactgtcgag tgcaaagccg gcgtggcaat gatgtcgtta
     9061 gccgtcgacg agccgcgtgg tgacggcggc gagaagtggt cgcgtgccat gtcgaacgac
     9121 gcgtggatga cgctgtggat gagtctcgag agcgcgcgct ggcgcgagct tgctgcggcg
     9181 tgtcctgaca ccggcgaccc gaacgtcggg accggaatca ccgacctcga gctggagctc
     9241 agcgacggca cgacaacgaa gaagctcgag tgctccgcga tgcggctgag ctcccagcac
     9301 ggacgggtga tcgatgcctt cgagaaggcc gccgaaatcg tcgacctcgt cccgtcgacc
     9361 tagctcaggg tgctgcgctg ccggccggcg cgggcgaccc agtgggcgtc gggccaccac
     9421 ccgcgggacc ggctcctgcc ggcggggtgt cgccggccgg aggcgcggct gcaccatgca
     9481 tcggaccggc gccgcccatc gcgcccatca tcacggtgcg cacctcgccc atcaccttct
     9541 cttgctgatc ggcggtgagc ttcgcgcgac aggccttcat cgacggcatg tcatgcgcat
     9601 cggtcgcgaa gcacttgatc gcgtcggcgt tccatttgtc gtcggtgcaa tgcttcgtgt
     9661 agatcgcgcg cagcttgtct ttgatgtcgg caccgccgcc acccgggccg cctggcatcg
     9721 agttgatcgc cttgtcgacg gcatcggcgc acgacggtcc actgggttcg gacttcttgc
     9781 cgcagcccgc gaacaggagg gcggcgacga tcactgcgtg cttcatgcgc ggcagagtag
     9841 cgcgcgttaa cgacgccgtg cgaccgcttg gcgcgcgagc tcgtcgcatc gcatgttgag
     9901 cgggacattt gcatggccgc gcacccagac aaagcggagg tctttgaact cgcgtgcggt
     9961 ctggcgaact tcggcgacga gctcgatgtt cttcttcgcc ttccagccct tcgtgagcat
    10021 gtcgatcgag aactgcgagt ccgtgtagac gatcaccggg cgcgtgcggt cctcgatcgt
    10081 ctcgagcgcg cgcaggatcg cggtgagctc cgccacgatg ttcgtgccgt cgccgagata
    10141 ctcggagagc tcggtctcct tgcccgcgtc gacaatcacc gcgccgatgc cggcggggcc
    10201 gggatttccc gagcacgcgc catcggtcca gacgtggatc gcgttcgccg gtgcttttgc
    10261 aatttcggca agcgcgtgcg cggtcgcgtt ctcttctgtc acgagcgcct tgccacgtgg
    10321 tgccgcaccg gcattgcggg tcggagtctt cgcgacagcg ccgttcgcag gcgccgcggc
    10381 gctcttctcg ggggccggtt ctccgacgac gatttcgacc ggcgcagccg ccgcgacctt
    10441 cgcgaggttg cgggcacctg cgcggtacac gcgcgcgccg tcggcgggct tgtaaacgat
    10501 ctccacacga ccgtcacgat cggtgacaag tgcaccgccc tcgtcgactt tcgcccatac
    10561 atcagcgtcg cggagtcggt gcttgagcca cggcatgccc gtgttataag ccacgggtcc
    10621 gatctcggac gccctagagg agaacgacaa tggttcgcat tcgcgttggt ttgctcgtcg
    10681 ctgttgccgc tctcggcctc ggcgtcggtg gttgcaagaa gaacgatgcc aagaaggacg
    10741 aaacggccgc cggcaaggcg acgacgggta gcgcgactac gaccgcgacg ccgaccggtg
    10801 gtggcgctct cgtcgcccct gcgggcagcg acgatctctc gctgctcccg gccgactccg
    10861 agatggttct cggcctgaac ttcgcccagc tccagcagag cgcgctgtgg aagcagttct
    10921 cgccgaagct gatggagaag atggagaagg gcctgaacga cttcaagacc gcctgcggct
    10981 tcgacccgat ggctgcggtc aagacggtct cgatgggcct caagggcatc ggcgcggatc
    11041 agccggacgg cacgatcgtc gtccatggtc tcgacaaggc gaagtcgatg gcgtgcctcg
    11101 acaaggcaaa gaccgaagcc gcgaagcagg gctcggagat caccgtcgac ggtgaagtct
    11161 tcacggtcaa ggacaagaac ggccagctca cggcgtggac gttcgtgaat gcggacacgc
    11221 tcgtcggtgc agtcggcccg acggcatcga aggacaccgt caccgcgatg gcgaagggca
    11281 cctcggcgct caagagctcg cagacgttcc tcgagatgta caacaaggtg aacacgaagg
    11341 agtcgctctg gctgttcgtg aacggcaacg cgccgttcat ggcgaaggcg gcgcaggcgg
    11401 gcgtgaagcc gaaggccgtg ttcggctcgg tcaacgtgac cgacggcctc acggtcgaca
    11461 tgaacatccg cctcggctcg gccgacgaag cgacccagct cgtcaacatg atgaagggcc
    11521 agacgcagag cccgcaggtc aagcagatgt tcgacaagct cgacgtcacg gccgttggca
    11581 ccgacgcgaa gatcgcggtc gcgatgtcga acgccaagct ccagcagctg atcggcatgg
    11641 tcggcggcat gatgggcggg ctcggcggcg gcgcgggcgg accctagtca ctcgtcgagt
    11701 cagagatgaa gcgcgcgatc acaacaacga tcgccgcgtg ctgtctggcg gcgacgctgg
    11761 tcgttgccgc gggctgcgga agtaagaagt cggggaaatc gcccgaggtc acaggcctcg
    11821 cggcggtccc ggcatccgcg caggtcgtga tcgtgaccga cgtcgcgcga gtgttgagct
    11881 cgccgatcat cgagcgcgcg atcgatcagc tgatgctgcg cgatgccgat ctggcggcgc
    11941 ggtggcagaa gctgcgcgac acctgcaaga tcgatatggc gaccatcagc cacgtcgcgc
    12001 tcgcgatcgg gcccaagacg ggtgactcgc caggcaccgg ccccgtgctg atggtcgtga
    12061 cgggcaagct cgtcgaagcg gatctcgcga cgtgcgtgcg cgcgatggtc gggcagggcg
    12121 gtggccagct gatcgcgaag gaagcgtatg gccacacgct ctatcaagcg gtcgacgcca
    12181 atcgcacgat gtacttcgcg ttcgggcggc cggacacagt cgtgctcggc tcgaacgaga
    12241 agtacgtcac cgaggcgctc gggaccggca cgaaggtcag cgacgatcct gagatgaagc
    12301 gttggatcag ccgcgcggat cagaaagctc ccgtgtgggc cgccggtcgc gtcgacgatc
    12361 gagtgaagac cggcctcgtc cgcgtgacga acggtcagct caaagaaggc ccgcaggcga
    12421 tgctcgtagc gctcgacccg acgaacggcg cgaagatcga ggtcagctcg gtgatggcgt
    12481 cggacgccga tgccaagacg ctggaatcat atgcgaagac gcagctcgga gcgctcgcga
    12541 tggcggcgca ggccaagcgg ctcggtcgcg tcgtcgagaa ggtccaaatc tcgtcggaaa
    12601 acgagaccgt gcggttcaag gcggacctcg ggatggacga ggtcaacttg ctgatttcag
    12661 tgcttgacgg cgaggcgccg cgcgagcaaa tttccccgcc cccagcgtcc ggttctgcaa
    12721 tcgggtcggg ctctgggctc tgaggtatca aatgtctagc aagcgcaccg aatcgaagaa
    12781 gaagatcaac acgaagaagg cccccgcgaa gaagaccgcg gtgaagaagg cgcccaagga
    12841 gaaggtcgaa gcgaagccca tgggcccgcg tcacccgaag gcgcgcgtcg ccggactcca
    12901 cgagggcaag gaagcgctcg cgaagacgct cgccgcgtcg ctcgccaccg agaccgaaga
    12961 cagcgactcg atcgcgagcc ggctgaagac ggcgtcgaac atgcagctgc ttcgcctcca
    13021 gaaggtcgtc gcgacggtga aggacaagtg gggcagccgc gagaagctga tcgcggcgct
    13081 cggcgagcaa gcgaaggcga gcaaggacaa ggacttcctc gcgaagctct cgacgttctc
    13141 gctgcctcgc ctcctcgaca tggcgcagtc ggcgacccgc cgcgctggcg cgtagtcgca
    13201 gcgttccgcg ctacgtttcg cagcatgcgc tgcacgtctc tgttgctggt gatcgcgctc
    13261 gcgcgtgtcg ccgcggcgga gagcgacgac gagatcgagc gaggagcacc cgaggttccc
    13321 gccggtgaat cgctcgtgcg tgccgatcaa gcggcatggc gctaccagct gatcaccgcg
    13381 cccgcgctcg cgccgcaggt cggtgcgctc gcattgagtg gcctcgacgt cgtcgccggc
    13441 cgcgcgcaac agccgatcga cgtgctcggc ggtggaccga cggtacccgc tgcgtggccg
    13501 tacggcctcg acacggtgac gcgcgcgacc gggacgctcg agccaacacc tccggcgcag
    13561 atgcggatcg cggcgatgta cgcgatcacg acgttcacgc tggcaccgga ggaccgaggg
    13621 ctgcgcgttc tcgagatccg cgtgcgctac cacgacggct gctcggttta tctcaacggg
    13681 atcgaagtgg cgcggcgatc gctcgccgac aacgcatcac cagtcgcgct tgcgcagcgt
    13741 ccgcacggtc cggaatggga gacgttctac gtgcctgccg caccgcatct ccttcggctc
    13801 ggcgagaacg tgctcgcgat cgaagtccac ccgtcgggca aacgggacgc gccggatctc
    13861 gcggcagacg tcgtcgcgcg acgcgagctc ggcgtcgttc gcggaccggt gctcgcggat
    13921 gtcaccgcaa cgagcgcgac gatcaacgtc gagaccgacg cgagcatcga cgccgtcctc
    13981 gagtggggaa ccgggacgac gctcgatcac aaggtctcga gcacggtcgg tcgcctgcac
    14041 gcgttcacgc tgacgaacct gccgccgaag acgaagctct cgtatcgcgt gatcgcaggc
    14101 gccgcgacga ccgcgacgta cgcgtttcac accgcgccgg gagctggcga cgtgatccgg
    14161 atcggcgtct acggagacgt gcgcggcggt cacgatacgc accggcgact tgtcgaagcg
    14221 atgctcggcg aaggcatcga cctcgtcgcg gtgaccggcg acatggtgct gcgcggcaca
    14281 gacgacgccg actggcagaa gttcttcgcc gtcacgcgcg agctgctcgc gcaaatccac
    14341 tacgtgcccg cgatcggcaa tcacgatgtc gggctcacga atggcgaagc gaacgtgttc
    14401 acgctgcctc ccgcgccgcc gggtcgcccc gagcgaacgt actggtacag catggagctc
    14461 gccgacattc acctcgtgtt cctcgactcg aacgcgtacg accgcagcga gcaggagcag
    14521 tggctcgacg cggacctcgc agcggcgcgc ggcaagaacg ttcgcgcgat cctcgcgttc
    14581 acgcacgacg gcccgtactc gcgcggcacg catcgcggca acgaaacggc gcgcgatcgc
    14641 ttcgtgccga tcctcgcgaa gtatcacgtc gatctcgtcg tcgccggtca cgatcatctc
    14701 tatcaacgcg gcgatgcggg cggcattcgc tacatcgtca gcggcggtgg tggcgctggc
    14761 ctctatcaag tcagctgcgg cgtgagcggc aaaccgaagt gcccggacga cggaatgcag
    14821 aaggtgctcg tcgaacatca ctacctcgtg ctcgcgatca cgaacgacac gctcgagatg
    14881 tgcccgcgca aggccgatgg tcgcctcctc gagaagtgcg tgcgctacaa gctgtggcgc
    14941 ccgtgacata ctcgacgcat gcgcgccttg ctcttgctcg tcgccgtcgc tggttgctac
    15001 cgcgaagcaa cgcccgcgag cgcaccgccg ccggcgaata agcagcagcc actcgtcgct
    15061 ccgcgggatg tcgacgacga gctctcgttt ctgccaaagg agtccgagct cgtgttcgga
    15121 ttcgacatga cgacgctgcg cacgagcgcc gcgtggcgcg agcagatcga gccggcgctc
    15181 gcagcgagca gcgcactcga aaagacgcgc aagctgtgtg gcttcgatcc gctcgcatcg
    15241 atcacgcatc tctcgatcgg cgcacgcaaa gcggacgctg gaaacgagct cgtcttcacg
    15301 ctcgcgggtg gcgacgcgca gcaacagatt ggctgcctca cgaagcagct cgatggcgac
    15361 tacgcagcgc aaccagcggg cgacgtcacg ctcatcacga agaagcagga caccttcgcg
    15421 ctcgcgctcg cgccggtcgg gcgtcagcac atcctcggtg tctcggcgcc gggcgtcgac
    15481 gcaaagcgcg tccgcgcaca agcggccatc ggctcgccac tgcgcgcgtc gcccgctttc
    15541 atgaacctct acgacaagct cgagcgcggc gcgtcagtct ggttcgtgct caacggcgct
    15601 tcgccgatgt tccagtcgtt ctcgctcggt gttcggccgc gctacatcga cggcaccatc
    15661 gtcatcacgg atcgctacgt gtggacaacg cgtatcacga tggagactcc cgccgatgcg
    15721 agtcagctcg cgacgatgtt ccgcggcgcg gccaatcagg tgaagcagat ggtcgagacg
    15781 ctcgagatcc gcgaggacgg cgaagtactg cacgtcgatg tggtcctgac ccagccgcaa
    15841 gtccaaacga ttctcgcgct gttgggcacc atgatctgac aggcactccg cgctggcgac
    15901 tcccgtttga tgcagtagag tgcccgcgtg gagcaacaag cccgcactgt cgacggagac
    15961 tcactctcgt accgcgtgtg gggccgcgcc ggcgctacgc cgctgatgac cgtgcacggt
    16021 ctggtctcgt cgacgcatca ctggctcgac ttcaacccgt actacgccgc gcatcgccag
    16081 gtcatctcgt gggagtaccg cggccacggt ggcaacccag cgccgcacaa cgcgcgcgcg
    16141 atcagcatcg cgcaattcgc cgacgacgcg tatcaggtgt ggcgcgccgc gggctcggag
    16201 cagcctgtcg tcgtggtcgg cctctcgttc ggcgtgcaag tcgcgctcga gctgtaccgg
    16261 cgccatcccg actgcgtccg cgcgctcatc ctcatctgcg gcacggcggg ccacccgctc
    16321 gaccgcgtct cggaatccgc atcgctgcgc cgcaacctca ccgcgcttct gcgaacactg
    16381 tcgtcgcagc gcattgtctc gacgccgctg ctcggcttct tgcgcacgcg cctgggcaca
    16441 cgcatcgcgc gcgagatcac gtatctctcg ggcggcgcga atcgcgacag ttgcccaccc
    16501 gcgatgctgc aaggcctgtt caagcatgtc ggctcgctcg atccggagct catcggcgcc
    16561 gtcatggcgg catacctcga gcacgacgcg cacgacgtgc ttcacacgat cacggtgccg
    16621 accctgatcg ttgccggcga tcgcgatcaa ctcacgccac tcgccgtcgc cgagcgcatg
    16681 cgcgacacga ttcccgggag cgagctcgtc gtgttcccgg gccactcgca cctcgtgcaa
    16741 gtcgagaagc cgcgcgaagt tcacgctgcg atcgacgcgt tcctcgcgaa gcatcagctc
    16801 tgagccgctg gagcgcggcc ggtcggactt gacgttgcgg caaacgcgaa ttatacattc
    16861 gcgtatgaag tcagcagagc tcgcgcgact ggatgccact gcgcaggcgg agctcgtcgc
    16921 gaagggcgag gtctcggcct ccgagcttct cgacgccgcg gagcagcgcg tccagcgcct
    16981 cgagccgttg ttgcacacga tggcggcggt tgacttcgcg cgcgcgcggg cggcgaggcc
    17041 ggcgaaaggg ccgtttcacg gcgtgccgtt cttgctgaag gatacgacgc cgtatccggg
    17101 cctgcgctgg gcattcggca cgcggctcat gcgcaacaac atggcgtcac cgccgacacc
    17161 attcgcgtcg cgtgtcgatg cggcgggctt cgtcgtgatc ggcaagagca cgatgagcga
    17221 gctcggcctg ctcggctcga cggagacact cgccgagggc atcacgcaca acccgtggga
    17281 tctctcgcgc tccgcgacgg ggtcgtccgg cggttcggcc gcggcggtag cggcggggct
    17341 cgtgccgatc gcgcacgcga atgacggcgg cggctcgatt cgcattcctg cgtcggcgaa
    17401 tggcgtgttc ggcttcaagc cgtcgcgcgg acggaccgtc acggcaatcg tcgggcgatc
    17461 ggatttcggc gacatcacga gcgatggctg cgtgagccga tcggttcgcg atacggcgcg
    17521 ctttctgtcg ctcgtcgagg acgacgtcag cgggtatcgg atgggctttg tcgcgggccc
    17581 cgagaagaag cggctgcgca tcggcacgtg gacgcagacg ttcgacggac gcgagcccga
    17641 cgcggaggtg ttgcgcgcgc acgcggaggc cgtggagctc gtgaaggagc taggacatca
    17701 cgtcgaggag gcgacgcctc cacggttcga gaccgaggtc ctcggcgatg cgttcttctt
    17761 ggtcgcgggc gctgcggtcg cggggctcgt gcagatggtc ggcggaatgc gcggcaagat
    17821 cgttcgcgac gatgagctcg agccgttctc gtgggcgctc gcccggcgcg caatggagcg
    17881 aggtccgtcg gcgctcgagg cggcgcgtga agaatttgcg agagcggcgc gcgcgcatct
    17941 cgacgcgacc gcgagctacg acgtcgtcct cacacccgtc ctcgcgacga agacgtggca
    18001 catcggccac ctctcgccgc ttgtgccggt ggacgagctg atccggcgca cggcggagac
    18061 ggtcgcgtac acaccgattc acaacattgc gggcgcgcct gcgatgtcgg tgccgctgca
    18121 cacgacgagc gacggcctgc cgctcggcgc tcacttcgcc gcaaagcccg gcgacgacgc
    18181 catgctcctc gcgctcgcgt acgagctgga atctgcgcgg ccgtgggcag atcgctggcc
    18241 ggggatctcg gcgccgatgc tgatggcgtg acatgccgca ggttctcaaa gacgaggtgc
    18301 gcacggcgat cgtcgacgcc gcgctcgcag ttttcgcacg cgacggcttc gaagcggcaa
    18361 cgatggcgag catcgcgaag gccgccggcg tcggtgctgc aagcatctac cgctattacg
    18421 agagcaagga cgagctgttc gacgccgtga tcacgccaga gctcacgaag cgcttcgagt
    18481 cggtcctcga ccgtagggtc agcgcgctcg cgcgcaatac gctgcgtggc gaagccgtcg
    18541 acgacgcggc gggcgagatg ctgcaattct ggatcgagaa tcggctcgcc gtcgtcatct
    18601 tgctcgaccg cgccggtggc acgcggtacg cgcactacgg cgagcgattc gtcgagctgc
    18661 tcgtgaagca cacgatcgcc gagctgcgcg ccgcgcaccg caacgtcgag atcagtcctc
    18721 gcgggcgctt cgtcatcacg cagctcttcg cgaacacgag gcgccttctc gcgagcgtgc
    18781 tcgccgaata ctcgcgtccc gacgagctcc gcgaagcgat cgagacattt tggagctatc
    18841 aagtggcggg actgcggggc ctcagggcgc atctgcgctg agtcacggac aatcctgcgg
    18901 gcagttcgtc acgtcttcgt gcacgccgca gcggccgttg ccgcagacgg acggcttgca
    18961 gctgtagttc ttggcgcgcg ccacacggcg caccggtccg agctcgtcgg cgcacacggt
    19021 ctcgcggaac ttgccgtctt cgaagcgctg cttcgtgacg agcccgaaca cgtcgctgcg
    19081 cggcttctcg cctgggtccg cgggctccga ttccttcagg acgaggaggt ctgcgggcac
    19141 cccacgcgcg acgagctcct tcttgacgcg ttcgagccga gcccgcgcgg tgcctgcggg
    19201 ctgcgcgagc gtctgctgcg tctgcagcgt cacttcgcga atctccggat acttctcgat
    19261 gacggcgacg acgtgatcga acgctcgcgg cggcgattgg tcgaacgcga tcggcgacag
    19321 ccagcaatcc gtgatgtcgg tgatgaacac gcagtcctcg tcggggcagc cgtgcaacgc
    19381 cgcaagcccc ggcgtgtcga agcacgtgtc ggtcgcgtcg gggacgccat cgcgatcgtc
    19441 gtcgagatcc gggcagccgt cggtgtcagc gcgcagatcg aagtcttccg gcttcgtcgg
    19501 acacccgtcc ttcgcgtcga cgatctcgtc gccgtcttga tcggcaaccg cgctcggcct
    19561 cggcgggtcg gctcgcgacg acagtgtcgc cggcgcttcg cggccaccgc acgctgcgac
    19621 gagcgcgacg agcaccagcc cgcgcattac tgatgcggtg gtggcgggaa ctgatgcacg
    19681 tgcgcctgcg gcagcatcgg ctggccgacc atcgtgaacg agccgccgag gaagacgatc
    19741 cacgcggggc gatacttgag cttgtacgag ccgcccggca tgaggtcgac gatgatcgtg
    19801 cccttcgact gctgtgacga gaacaggtac ggataccaag cctcgaagac gtgccgaccc
    19861 ggcggcagtg tcagctgcac cttgccccat gactgcttct gcgcgtgacc gttcaccgcg
    19921 agccacggcg aaaccagata caggatccac atcaatggaa agaaaccgag gtcgacctcg
    19981 acgtacgcgg gctgcatgcg cgcagcgtac aggatcgcgc gatgcggtag gatggtggcg
    20041 ttgcgttgga tgctggtgat tgggtgcgtg gcgtttgggt gtcgcgtcca cttcgatgag
    20101 cggcgcgacg ccggcggtcc ggttgcggat gcagcagcgc cgtgcgttgc cggtgagtgc
    20161 agcccgtcga ccacgttccg tgtcacgtgc tcgaccggac cgaagagcgc cgacgagatc
    20221 tgcggaagca tgggcttcgg tacgacggcg gtggttcgcg gctattactg gttccaatgc
    20281 ggcggaacgc tcgaccggtg tcccggcggg tgggacggca tcgactgccc ggcgtggtgc
    20341 ccaggcggca ccgactgcgt cggcgtatcg ttgtgcggct ccgccggtga gtccctcgtg
    20401 accgagaaga cgggcgacgg gtcgacgacg ttcgatccga tggaaaccgg gcgaacgtgc
    20461 atggggtaca accccggttg gaccgttcgc atccgctgct acctgcggtg accgtcgccg
    20521 cccctaggtc gcggccttct cgtcgccggg gagcgggact ttcagcgtcg cgagcgtctt
    20581 gcggacgatg ccccagaccg cgcccgcatc ggtcgtgatg tcgtcccacc ggccggggaa
    20641 ccgggcgcag aacgatgtct tcatgatctc gggcagcgcg cgcttgccgt cgtcggcgaa
    20701 cagcggatgc ttcaagtaga cgcaggcctc gtcggtcgcg cgcaccgaga gcgagccggg
    20761 caagtcggcg gcggtgcgga tcgcatcggc gaccgcgacg atgatctcga gattgctcgc
    20821 ggagacttcg gggtggcgac gcgcgaggat ctcgacctca gctttgggcg gcgggtagtc
    20881 catctgcagc ggcgcgaagc gatcgagctg cgccgcgtcg atgccgaacg tgccggtgaa
    20941 ctcgcgaccg cgattgacgg cggcgatgaa ctgcacgttc tcggggatcg ggatgaacgt
    21001 gttctcgatc ggatggaaca cgcgccgcgt ggagtcgagc gcgggcatca gcgcgttgcg
    21061 cgcggattcc tggcaacggt tcagctcgtc gagaaatacg aggtacttgc ggtctgggtg
    21121 ttcggccgct tcttcgagcg cgacgaggat gtcggtcttc acgaagtcgg tgaccggggc
    21181 gcctgactcc gatgcgcgaa cttggatcga gacgaagaag tccgacgcct cgacgaccgc
    21241 gccgcagttg aagaagacga acaccatgcc gagcgtgtcg gcaatcgacc gcgcgagcac
    21301 ggtcttgccg cagccctgcg gaccgtcgag gaggatgttg aggccgctcg tcagcagtac
    21361 ttgaagcttc ttggagacga ccggatcgag atacatgccc tcgatgcgga acggcgcacg
    21421 cgagacgaac tcgacctcga tgcggccgcg atcatcgcgt tcgggtttgt ggatctcgag
    21481 cacgcgcacg agacacggga cacccgccac gatgcggtcg tcattcgaga ggacgacctt
    21541 gggcgcacgc cggccatcga ggtgcgtcgc gcgcagcttg aactgcgagc cctcggcggt
    21601 gttcggccag aagattgcct cgacggtgcg cccgggcgtc agctcttcga cggtccactt
    21661 cgacatgact gcctcatgat gccagtgagc ggcgcgcgag gtcaccgatc atgcggccga
    21721 acttcgcgac cgcgatgtcg agctcgctgt cgtcgaggac gaggtagttc gggaagcaga
    21781 cctcgtcgag cttacgcacc gcgacctgcg cgcagacgat gttcttgcgg cgagtcagcg
    21841 tgacgacgag gcctttgagc gccgcaaccg agcactccgt gggcaggcca tcgctgatca
    21901 tgacgaggac cttggcccgc ttgcctgaat tcagcgcgac gttcgccgcg tgatagagcg
    21961 cggccgcgtc gttgttgcca ccttctgact gcagcgcgac gacgttgcaa tcggtcgccg
    22021 ttcccgcgtc gtagatgaca gagtcggtga agccgaagaa gcgcgcttcg acgccgggca
    22081 gccgcgcgac tgcttctgcg acgaggatgg cgaaccgcct cgcacgatcg aggttgttgc
    22141 ccgcctgcat cgagccggag caatcgatga tgacgccgag gaataggtcc gttcggcgaa
    22201 tgggattgcg agcgatcaag atgcgaggat cgttgcgcgt gacgagtgcg cgcaggcgcg
    22261 tgcgatcgag tgcgcggcct tgcgtgcgtg cgcgctgagg ttcccagcgc aggccgagct
    22321 cgtcgagatg ttggcgcaag cgcgcggcat ggcgcgagac gttggtggcg aggttgcgat
    22381 gcacttcttg atcgccgcgg acgcgcatga cacgcgtgat cttgtcgaag tgctgatcgg
    22441 ggctgacgtt gagctgtagc cgcggtgggc cacctttatc gctgcgtttg ctcttcgaac
    22501 gccgcggatc gaggatgcgc tcgacctcgc gctggaggat ttcgtcgtcg atgccggcgc
    22561 cgaagacgtc gtcgtcgcgc tcgccgaaca cgaggccctc ggggccgccg aacacctgcg
    22621 cgaggcgtgt tgcgccgccg aacagcgtcg cgagctcctt cgtgagcgca tagaggccgg
    22681 ccatgtcgag cgtgcgcaga tccttgccgc agagcgccag cgcctttgcg acgagcggat
    22741 cgtttgcgcg attgccaagg cccatgcgca gcgcgcgaga gaatcttgcg accggatggc
    22801 cattgcgatc gagctcgccg aggatagcgc cgcgtcgcat gcgaacggat tgctcgtcga
    22861 aggcaacctc gaggtctgcg gcgatgaggg cgggtgctgc gaagccgcgc aacgacgcga
    22921 gcagcgcggc aagcttgatc tcctgcggtg cgtgttggaa cgcgtatgcg ccgagccgct
    22981 tcagccgatt gccatagtcg ggatcgatcg cgcgcaggtt tcgctcgagg tgctcgtcgg
    23041 cgatgaggtt caagaagtgg ccgagaccct cgtcgtgcgc ctgcttccag atcttctggg
    23101 cctccggaga cgcgtgatag atgtgatgcc cgatctcgtg gaggacgagg ccttcgacga
    23161 tgtcgcgccc gtgcgtctcg cctcgcagca tcggcagtgc cgtgacgaag atgcgcgagc
    23221 cgtcgagctt cgtgtggccg aagtcggtct ccttcgcggt gaggtgaaac gcgaacagct
    23281 tgcctgcgag ctcgacgccg cgacgcacgc cccatgcgaa cacgtcggcg acgcggcgca
    23341 ggcggtcgtc cccagtctcg aacgcgaccg cgaactttgc agcggcgcgg cgcgcggccg
    23401 gcgtcgacgc gtgctcgagg attcgcatgt cgagctccgc gagcactcgc gcggagaggc
    23461 ggctgcgatc gcgaaggcgc tggacgagct cccacatgcg acgctcgcta cagacggtgt
    23521 ggccgccgat cagtgcagcg tcgacgatcg ttcgtgcgac gccatcgagc gcacgcgcag
    23581 gtacgagctc gagcgtgaac atccagtcct gcgcgcgctc gtccgcgatg tactcgacga
    23641 ggagcggcaa tcctgtcgta tcgtcgagct cggcgagccg ccgcgcgacg gcgacgcgca
    23701 ggtgcagcgg ccggtccccc gatgcttcga gaaagcggac gagcgcggcg tgtacgtcgt
    23761 cggtggggcg cgtgagcgcc aatagaactt ggatcgcgcg ctgatacgca tggtggtgcg
    23821 gcgcgtccgc cagcgcgacc gagcaggtga ccagatcggc gacacgaacg agcgcctccc
    23881 agtcatcgcg gcggaagtac caggtgcccg gcgtcgcgcg aactgcagcg agcgcatgct
    23941 cgagtgctcc gggctcgcca cgcgcatgca agctgagctg gatgtaaaac tgccacgcag
    24001 ccggcagcga cgacgcgagc acctcgcgca ccatcacgat ggcgggttcg tgctcccaca
    24061 ggaggattga cgacagcaga ggcgtcggcg cggggaggac cgcttcgggc gctggctcgc
    24121 cgacgtcgtt gcggaacgcc gcggcatcgc gcaacagctc ggcgagcttg atctctccct
    24181 tctccccgag gacgacgagc gcgagcgccg cttcctgcac gacctgcggc cgcggatcgc
    24241 gacaccattg tgcgaggcgc tggatatcgg tgcatgccct gacggcatcg aggaccacgg
    24301 cgggcggcgg cggtgcaggg ggtggtgccg cggccacacc gacgaggcgg acgaaccgcc
    24361 cgacatactc gcgcgtcgcg acatctgcat cggccacgcg gtcgagcacg gcatcgcgaa
    24421 cgctctcgac ggggatgatg cggctctcga cgagcgcgac gaggatgcgc gctgcatggc
    24481 ggccgatatc ccggtcgatc cgcacggcgc agcacttcgc gagctcctcg gtcgcatacg
    24541 agcgaaagcg ctccacgtcg cgatagcgga cgagcatgag cgcctccgag accgcgcgcc
    24601 acaacgtgtc gcgagcgacc ctgccgggca gcgcttcggc gcgcgcaagc accgcgacga
    24661 cgctcggctc gcgatgcagc cagtccaaga atgcgaacaa atgctgctcc cagcgccaga
    24721 gtcgcgcgag cgcgagcggc ggcaagtcag cggtcgggac gcgacgccac gtgcactcac
    24781 cgtcgtcgag cgcggcgtcg aacttcgacg tcgcttccga aaaacgatcg agtgccttcg
    24841 cgacatcgac gagcggatca gcgcagccga gcagggcgac gcagatcggg atgctcggac
    24901 cggcgtcgcg gcccgagacc gcggcgacaa ggccccccac cgcaccggtg cgcgcaggcg
    24961 cgagcgcagc ttcgaggtcg cgatcgttac agcgcgcgat gcgctcgcgt tcagccgcgg
    25021 ttaggtcgcg cttcgccgtg acctgcggag ccagtggcgt ctccggcgcc tcggccgtca
    25081 cgtccgtgcg ccacttgatg atcgcggcgt ccttatggcg cgcgaacgct gcgacgaggg
    25141 caagctcttg atcgcgtggc agcggttcct cgctgacaat ctcgacgatg cggcgcgcga
    25201 gcggctcgac gaggagctca gccgctgcga acagatcgcg agctggaagc gcacgcaaca
    25261 gcgcgtggag cacgttcgtc gcgctgagcc cttgaatcag cgaagcggcg gtcgtgcgat
    25321 cgacgttcgc agcgacggtc ttgcagacga cctcgagcgt ctcgccacca gcgtcggcgt
    25381 acagcgcgag tgccgcttca aacgccggct ggcgatggcg ccggggcaac agctggacaa
    25441 accgctcgag gcgtttgtgc gtgagcacac cgagcgcgcg cccggcgagc tggatgtatc
    25501 gcttgtagtc ctgcttctcg acggcggcca gccagcttgt ctcgagtgcg ggcacctcgc
    25561 cgggaagccg caggatttgc tcccagccgt gatcatcggc gacgaggcgc gcgacgatct
    25621 ccgcttgtcc gagcttctcg cggagcttgt cgaggcgctt cgcgggcaac agcccggcca
    25681 ccgctgcagc gagcgcgagg tcacccctca cgacgtcgct cgcgagcagc cgcatgatct
    25741 gcaagctcgt cggcttgacg ggccaccgat agcgaaacaa cccgcgcgtc gcctcgaacg
    25801 caaggcgcgc gtcgaaccca gcaaactcga tcacccgcgg ctcgagcgcg acgcacgcac
    25861 tcacgagcaa cggctccggc ctctccgtgc gcgcccaata gcgcagaagc gcgaccgccg
    25921 ccctccgccc gagcacgccg ccgatcgcct tgttcggcgc gacgcactcg acgaacgtat
    25981 cgatcgcacg aggcacggtc ctctgcgcta gcgcatcgac gacaacgtcg agcacatcgc
    26041 gtcccatcgg ctcggcgatc acgccgcgct cgagatacgc atcgacgata tccgcgccgc
    26101 gaccgacttc gcgcttgtag agctcgcgca gctccgtcgg gctcgcacgc gtcagcgcct
    26161 ccaccagctc gctcgccggc acatgccccg cctcgtagag ctccagcgcg acgggcaatg
    26221 catcgtcggg ccagcgcagc ttgctcgcga ggtccgcggt cgccggatcc gcgcgcagat
    26281 agatcgcgag cttgccgagc gacagcgaca gctcgccctc gagcgccgca cgccgcacct
    26341 cgggccgcga atgaaacagc gcatgcgcgt accgctcggg cacatcgcgc gcgacggctc
    26401 gcaacgcctc gagcgccgcg cgccacacac tctcctcggg atcatcgagc cgcccgacga
    26461 tcgcgcgcac gccctcatat ccgcgcgcac ccgcaagcgc ggccaacgcc ttctctcgca
    26521 cggccgcgtc tccactgagc actagcggct cgagcgcgcc tagatacgcc acgcgccgcg
    26581 ccaacggcca gctcgcaatc tcctcgagat ccgcgctgcc gacctcgccg ctggcgagcc
    26641 gtctatagat ggcgaggtcg gtcatctcat ctcatctcgt ctcatcccca ctcacgtcgt
    26701 aacaaattcc gggcgtcgcg gctgcacgtg tgtcgcctcg atcgtcgagt cgctcgaagg
    26761 tgtcctcgca cgcacacagc cgcatgctcg gacgaacgcg ccctcgacga tcgcggaagc
    26821 acagcccgac tcgaagtcag agcctgcgtg acgcgcggcc acacccgcct ggcgcaatgc
    26881 caagcgagct agtcgcacat cacagcgaaa gccccgaagt c
//
LOCUS       DATLBZ010000353         5967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1904636, whole genome shotgun sequence.
ACCESSION   DATLBZ010000353 DATLBZ010000000
VERSION     DATLBZ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904636"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..941)
                     /locus_tag="VMZ53_18270"
     CDS             complement(183..941)
                     /locus_tag="VMZ53_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30464.1"
                     /translation="MARATWVFVATSAIAAAAAIWLYLDNADLRAQLAASKPTATSNG
                     SASDDDDGKPAKPTGDTWASSSEPSGSSGAAISAGPGPRLPEQKGESRLERRQRRMEE
                     FSAMFGRLDGETEEEYRNRIVPLIKGGLLIPRQRVEDMRKEIEQKANVSPEQSKQLDQ
                     AFDKVYADALDYTNKAVADGLLSPYERNVSGWLEYAGGMGGILNDANGQIGKILTPDQ
                     MKTIGSSGFEWGEYLGLNAPWENLKPPPPQKPTH"
     gene            966..1487
                     /locus_tag="VMZ53_18275"
     CDS             966..1487
                     /locus_tag="VMZ53_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30465.1"
                     /translation="MERKPELRLPTTAVPVTLWLAGGVNIDAELFVGEMARTGHSQLL
                     DDVATMLDETASFLPVRVERAIQLYAKRAILVVSVVRRDDDKDKDDGGDEFDDVPSEV
                     FSLYDRQHHVKVELAGERSLSGMLFDSSPSDRPRVIDHLNGGGRFLRLWTEGTHYLIN
                     KDQIIRVSEQEPT"
     gene            1487..2812
                     /locus_tag="VMZ53_18280"
     CDS             1487..2812
                     /locus_tag="VMZ53_18280"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HUS30466.1"
                     /translation="MARIEVYFRSIEKFGASGAILTSGQAVTMRFPQGDRHATQVTPH
                     DQLIGMVREVAPPAVLTQIDAGRPAKFDVESAGVRYSLTIQPKPNAWQVLIEGIGGGS
                     GQTQPSAAPIAAQPATQSGQVPRAQTSRAQTAPSEFAEMTIERGQYAEPAGPRSTSGS
                     VFLDQLTAAARTARATDVYLATGAGPAARVGNDMQPLGDTRPLDAETISREIGIVAPA
                     DARASWTERGLGVFTYGDGVGRVRATLTRDHRGPSATLRLLVGEPPAVDRLGIGREAA
                     VWLDARGGLILVAGPSGSGKTTTLAALVRGLGEKRRRVVTFEDPIEIIHVTSPWISQR
                     AIGEHVPSIAAGVATAMREAADAIVLGSIATAEAANATVDAVAAGHLVLGTISASSAR
                     HAADQLVDLLPYDRRDLARGALEHSLLGTIAPVVKGGGRSFEVVAGRSG"
     gene            2847..3779
                     /locus_tag="VMZ53_18285"
     CDS             2847..3779
                     /locus_tag="VMZ53_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HUS30467.1"
                     /translation="MTDDYRDGGTLILQRPLGPPEKKEKISGEACLVNLHPPGPDIGK
                     RVPLVNTQYVVGRDQDAGFVVSRSSVSRQHARLFVDDDGNWWVEDLNSTNGTFVNENR
                     IRSQQLSDSDQVRFGDAIYKFLSGSNIESAYHEAIHNMAIQDGMTGIHNKRFFMEFLE
                     REIAVASRHGHPLTLVMFDVDHFKKVNDTHGHLAGDAVLKDLAGRIRPRIRREDLFAR
                     YGGEEFACVLPSTALPGGIVFAEHLRTLVEERPCAFDGGAIPFTISLGVTTLHREAGV
                     DPAGLIKRADENLYAAKRGGRNRVVPSLADLIPA"
     gene            3781..5208
                     /locus_tag="VMZ53_18290"
     CDS             3781..5208
                     /locus_tag="VMZ53_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30468.1"
                     /translation="MRVAVALVCLIACGRSGFDPTDAGDGPNGDASIVTPRCTVPARP
                     DVASPRITLGCDPIVAAQEILKGGVIRFDCAMGVQLGAVILVNKDTVIDGHGVVLDGQ
                     GITKVFLVNNNAHLTLLDVTVTRGASSDDGAAVTLDSIAGGLVAFDSTFTDNHAAAAN
                     QSGGAIAARPAQPTVEIYGGRFSNNTSASGGAVSAFSNLTIMNASFDNNVAVGTGGSD
                     GEGGAVQFAGTGAVTMCGDTFTANVGAHNGGAIVMISIDNSSIMQMTDVVVDFNRAAG
                     AGGAYLQGFTTLTMQRVSITSNAATAGPGGGLWLSGTSGMFTGENVSIVANRADNGTG
                     AGMFAGDVPGRFSFSTIADNFGNCAGCNSAAIANGGNIDYRATIIVDNASQTTPAACA
                     ATGTNSGGTWQWPGDLPLCVAGATVTDPLILPRGNATGPAGTLLVRRPSSGSPAIGAV
                     TSGCPTEDVLGNPRATPCAAGAVEP"
     gene            5220..>5967
                     /locus_tag="VMZ53_18295"
     CDS             5220..>5967
                     /locus_tag="VMZ53_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30469.1"
                     /translation="MAPLIGDVMIAAKTLEDLGWPTLVEHWAKRCATTRGANAVRTNR
                     LFDAIDAARERAHEIREARDLASRDASLPLGDISDIAPSIERVRKSAALDAPELVAVA
                     RTGRSLARLRTHLREHADVAPKLAAHGAQLADLGHVFHPILDAFDADGRLVDHASDAL
                     GGLRRAVASIRAMLVQRMDSMLTDEKFAPYLQDSYYTQREDRYVLPVRTDGKGFVKGI
                     VHGTSQSAATMFIEPEEIVDLNNRMKLAEAE"
BASE COUNT          999 a         1941 c         2013 g         1014 t
ORIGIN      
        1 tggcagggga cgcgggggac gcaggtcgga gctcgtcaca cgagcacgag cacgagcacg
       61 agcacgagca cgtgacacgt ggacgtggac gtgacacgtg tacgagcacg tggacgacgc
      121 acgcgcgcgg ccgtcgtcgc cgacgccacg ctgcgcagcg ccgagctgaa cggcgaatcg
      181 gctcagtgcg ttggcttttg cggcggcggc ggcttcagat tttcccacgg cgcgttgagg
      241 ccgaggtatt cgccccactc gaagccactc gacccgatcg tcttcatctg gtcgggcgtg
      301 aggatcttgc cgatctggcc gttcgcgtcg ttgaggatgc cgcccatacc gcctgcgtac
      361 tcgagccagc ccgacacgtt gcgctcgtac ggtgagagca ggccgtcggc gaccgccttg
      421 ttcgtgtagt cgagcgcgtc tgcgtagact ttgtcgaagg cttggtcgag ctgcttcgat
      481 tgttcgggcg acacgttcgc cttttgctcg atctctttgc gcatgtcttc gacgcgctgg
      541 cgtgggatca gcaggccgcc tttgatcagc ggcacgatgc gatttcgata ttcttcctcg
      601 gtctcgccgt cgagacgacc gaacatcgcc gagaattctt ccatgcggcg ttggcggcgc
      661 tcgaggcggc tttcgccttt ctgctcgggg aggcgagggc cggggcctgc agagatcgcg
      721 gcaccgctgc tgccagacgg ctcgctgctg gaagcccagg tatcgcccgt gggcttcgcc
      781 ggcttgccgt catcgtcgtc ggaggcggag ccgttgctcg tcgctgttgg cttcgaggcc
      841 gcgagctgcg cgcgaaggtc cgcgttgtcg agatagagcc aaatcgcagc cgcggcggcg
      901 attgccgacg tcgcgacgaa gacccaggtg gcgcgcgcca tagcgaaaaa gctagcatgc
      961 ctgcgatgga gcgtaagccc gagcttcggc tgccgacgac cgcggtgcca gtcacactgt
     1021 ggctcgccgg cggtgtgaat atcgacgccg agctattcgt cggagagatg gcgcgtactg
     1081 gccacagcca gctactcgac gatgtcgcga cgatgctcga cgagacggcg agcttcctgc
     1141 ccgttcgggt agagcgagcc attcagctct acgccaagcg agcgatcctc gtcgtttcgg
     1201 tcgtccgccg cgacgacgac aaggacaagg acgacggcgg cgacgagttc gacgacgttc
     1261 cgtcggaagt gttctcgctc tacgatcgcc agcatcacgt gaaggtcgag ctcgcgggag
     1321 agcgttcgct cagcggcatg ttgttcgact cgtcgccgtc ggatcgcccg cgcgtgatcg
     1381 atcatctcaa tggcggaggc cgctttctgc ggctgtggac ggagggaacc cattacctga
     1441 tcaacaagga tcagatcatt cgcgtgagcg agcaggagcc cacctgatgg cgcgcatcga
     1501 ggtctacttt cgatcgatcg agaagttcgg cgcgtcgggc gcgatcctga cgtcggggca
     1561 agcggtgacg atgcggtttc cgcaaggcga tcgccacgcc acgcaggtca cgccgcacga
     1621 tcagctgatt ggaatggtgc gcgaggtcgc gccgcccgcg gtgctgacgc agatcgacgc
     1681 ggggcgtccc gcgaagttcg acgtcgagtc cgccggcgtt cgctactcgc tgacgatcca
     1741 gccgaagccc aatgcgtggc aggtcctgat cgaaggcatc ggcggcggca gtgggcagac
     1801 gcaaccctcc gcggcgccga ttgcagctca accagcgacg cagagcgggc aggtgcctcg
     1861 cgcgcagacg tcccgtgcgc agaccgcacc gagcgagttt gcggagatga cgatcgagcg
     1921 cggccagtac gccgagccgg cgggacctcg ctcgacgagt ggcagcgtct tcctcgatca
     1981 gctgaccgcc gcggctcgca cagcgcgtgc gaccgatgtc tatctcgcga ccggtgcggg
     2041 acctgcggct cgcgtcggca atgacatgca gcctctcggc gacacgcgcc cgctcgacgc
     2101 cgagacgatc tcgcgcgaga tcggaatcgt cgcaccggca gatgcccgcg cgtcatggac
     2161 ggagagaggc ctcggcgtct tcacgtacgg cgacggcgtt gggcgcgtcc gggcgaccct
     2221 tacgcgggac catcgggggc ctagcgctac gctgcggctg ctcgtcggtg agccgccggc
     2281 ggtcgaccgt ctgggcatcg ggcgcgaggc cgcggtgtgg ctcgatgcac gtggtggcct
     2341 catcctcgtg gccggcccgt cgggcagcgg caagaccacc acgctcgcgg cgctggtacg
     2401 cgggctcggc gagaaacgcc gccgggtcgt cacgttcgag gacccgatcg agatcattca
     2461 tgtgaccagc ccgtggatca gccagcgcgc catcggcgag cacgtaccgt cgatcgcggc
     2521 gggcgtagcg acggccatgc gcgaggcggc cgacgccatc gtccttggct ccatcgcgac
     2581 cgccgaggct gccaacgcca ccgtcgatgc cgtcgcggca ggccacctcg tgttgggcac
     2641 gatttcagcc tcgtcggccc gccacgccgc cgatcaactg gtcgatctct tgccttacga
     2701 tcgacgggat ttagcccgtg gtgccctcga acacagcctg ttggggacaa ttgcacccgt
     2761 agtgaaaggt ggcggacggt ccttcgaggt cgtggccggc cggagtggct aaagccccca
     2821 aagccgcgat atataagaat gtaggagtga ccgacgacta tcgagatggc ggcacgctga
     2881 tccttcagcg tcccctcggg ccacccgaga agaaggagaa gatttcaggc gaggcgtgcc
     2941 tcgtcaatct tcatccgccc ggtccggaca tcggcaagcg cgttcctctc gtgaacacgc
     3001 agtacgtcgt cggtcgcgat caagacgcgg gcttcgttgt cagccgctct tcggtctcgc
     3061 gtcagcacgc gcggttgttc gtcgatgacg atggcaactg gtgggtcgaa gatctcaact
     3121 cgacgaacgg cacgttcgtg aacgagaacc gcatccgcag ccagcagctc tccgacagcg
     3181 atcaggttcg cttcggcgat gcgatctaca agttcctgtc gggctcgaac atcgagagcg
     3241 cgtaccacga agcgattcac aacatggcca tccaagatgg catgacggga atccacaaca
     3301 agcgcttctt catggagttc ctcgagcgcg agatcgccgt cgcgagccgc cacggtcatc
     3361 cgctcacgct cgtgatgttc gacgtcgacc acttcaagaa ggtgaacgac acgcacggcc
     3421 acctcgctgg cgatgccgtg ctgaaggatc tcgcaggccg cattcgcccg cgcattcgtc
     3481 gggaagacct gttcgctcgt tacggcggcg aggagttcgc gtgcgtattg ccgtcgacgg
     3541 cgttgccggg cggcatcgtg ttcgccgagc acctgcgcac gctcgtcgaa gagcggccgt
     3601 gtgcattcga tggcggcgcg attccgttca ccatcagcct cggtgtgacg acactgcatc
     3661 gcgaggcggg cgtcgatccc gctggcctca tcaagcgcgc cgacgagaat ctgtacgcgg
     3721 cgaagcgtgg tggccgcaat cgcgtcgtcc cgagcctcgc ggacctgatt cccgcgtagc
     3781 gtgcgcgtcg cggtcgcgct cgtttgtctg atcgcctgcg ggcgctctgg cttcgatccg
     3841 acggacgctg gcgacggtcc caatggtgat gcgtccatcg tgacgccacg ctgcacggtg
     3901 ccagcgcgcc ccgacgtggc gagcccacgg atcacgctcg ggtgcgatcc gatcgtcgcg
     3961 gcgcaggaga tcttgaaggg cggcgtgatc cggttcgact gcgcgatggg cgtgcagctc
     4021 ggcgcggtca tcctcgtcaa caaggacacc gtcatcgacg gacacggtgt ggttctcgac
     4081 ggtcagggga tcaccaaggt gttcctcgtc aacaacaacg ctcacctcac actcctcgac
     4141 gtgacggtga cccgaggcgc gagcagcgac gacggcgccg cggttacgct cgattcgatc
     4201 gctggcggtc tcgtcgcctt tgattcgacg ttcacggaca accacgccgc tgctgcgaat
     4261 cagagcggcg gcgcgattgc ggcgcgtcca gcgcaaccga ccgtcgagat ctatggcggc
     4321 aggttcagca acaacacgtc tgcgagcggc ggcgccgtct ctgcgttctc caacctcacg
     4381 atcatgaatg cgtcgttcga caacaacgtc gcggtgggca ccggtggttc cgatggcgag
     4441 ggtggcgcgg tgcaattcgc gggaaccggc gccgtcacga tgtgtggcga tacattcacg
     4501 gcgaacgtcg gcgctcataa cggcggcgcc atcgtcatga tctcgatcga caactcgagc
     4561 atcatgcaaa tgacggacgt cgtcgtcgac ttcaatcggg cggcgggcgc gggtggtgcg
     4621 tacctgcagg gattcacgac gctcacgatg cagcgcgtgt cgatcacgag caacgcggcg
     4681 accgctggcc caggtggcgg gctctggctc tcgggcacga gcggaatgtt caccggtgag
     4741 aacgtgtcga tcgtcgcgaa tcgcgccgac aacggcaccg gcgcgggtat gtttgcgggc
     4801 gacgttccgg gacgcttctc gttctcgacg atcgcggaca atttcggcaa ctgtgccggg
     4861 tgtaactccg cggcgatcgc gaacggcggc aacatcgatt accgcgcgac gatcatcgtc
     4921 gacaacgcgt cgcaaacgac gcctgctgcg tgcgccgcga ccggcacgaa cagcggcggc
     4981 acctggcagt ggccgggcga tctcccgctg tgcgtcgcgg gcgcgaccgt gaccgacccg
     5041 cttattttgc cgcgcggcaa cgcgacggga ccggccggaa cgctactcgt tcggcggccg
     5101 tcatcgggaa gccccgcgat cggtgcggtc acgagcggtt gcccgaccga ggatgtgctc
     5161 ggaaatcccc gcgcgacgcc ctgcgctgcg ggtgccgtcg agccctgaag ctgctatcta
     5221 tggctcccct catcggggac gtcatgatcg cggcgaaaac tctcgaagac ctcggctggc
     5281 cgaccctcgt cgagcactgg gcgaagcgct gcgcgaccac gcgcggtgcg aacgccgtgc
     5341 gcacgaatcg gctgttcgat gcgatcgatg ccgcgcgcga gcgtgcacac gagatccgcg
     5401 aggcgcgtga cctcgcatcg cgcgatgcgt cgctgccgct cggcgatatc tcggatatcg
     5461 cgccgtcgat cgagcgcgtg cggaagtcgg cggcactcga cgcgccggag ctcgtcgcgg
     5521 tcgcacggac gggcaggtcg ctcgctcgcc tgcgcacgca tctgcgcgag cacgcggacg
     5581 tcgcaccgaa gctcgccgcg cacggcgcgc agctcgccga tctcggacac gtgtttcatc
     5641 cgatcctcga cgcgttcgat gccgacggcc ggctcgtcga tcacgcaagc gatgcgctcg
     5701 gtgggctgcg ccgcgccgtc gcatcgatcc gcgccatgct cgtccagcgc atggacagca
     5761 tgctcaccga cgagaagttc gcgccgtacc tgcaagacag ctactacacc cagcgcgagg
     5821 accgctacgt gctgccggtg cgcaccgacg gcaagggctt cgtgaagggc atcgtgcacg
     5881 gcacgtcgca gagcgcggcg acgatgttca tcgagcccga agagatcgtc gatctcaaca
     5941 accgaatgaa gctcgccgag gcggagg
//
LOCUS       DATLBZ010000354        11215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4121911, whole genome shotgun sequence.
ACCESSION   DATLBZ010000354 DATLBZ010000000
VERSION     DATLBZ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4121911"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VMZ53_18300"
     CDS             complement(<1..874)
                     /locus_tag="VMZ53_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30470.1"
                     /translation="MRTKLNSLRTVLAALLVAGGVGAACSSSSHQRSPESTTVAQAPL
                     GQSTAEYPSDPSVAPPDAAEAPEQQVGPVHSLSSGAVNGATTAPGANGGTPTNGSSPT
                     NGSAPTNGSAPTNGGTPTNPSIPAPTPASPNAPSSTPAPNPMPPSGGEPVQGAPNTGT
                     TGTGPTGTGTGTTPQSTLRGLSSEVAPRARDSASVNRPMISTPSVADAFGETADPYQT
                     RADAGVAPPTQPRNDAGVGSGRGDAGIGGGGGGGGRGDAGIGGGGRGDAGIGGGGGGG
                     GRGDAGIGGGGRGDA"
     gene            1022..2071
                     /locus_tag="VMZ53_18305"
     CDS             1022..2071
                     /locus_tag="VMZ53_18305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HUS30471.1"
                     /translation="MPARAIDTATIAFGLVSIPIKIYSTSEPTHEIHFHLIHEGCGER
                     VKQQYTCPRHGVVERSEMSKGFELTKGNFVELSKEELKALEAVASDEVSIREFIPAVA
                     IDPIYVDRTYFLGPGKGGERAYRLLRDALEAAELVGIAAYAARGKEYVVMVRPFEDGL
                     AMHQLRYPDEIKSWSEVPVGRLPKAAPAELQLAGQIISQLRHDTFDPTQYSDEVKDRV
                     RKLIAKKAKGGEITAPPEAPKPVVTDLMEALKASLSGEGATKPAAAGSASRSRKNGHA
                     RNGNGATKSTTHARSSTTKSAHGARGGGSKATSESSGRSRGHAHAKTRATRPAARPHR
                     APTRAARSQSKRAHR"
     gene            complement(3015..3944)
                     /locus_tag="VMZ53_18310"
     CDS             complement(3015..3944)
                     /locus_tag="VMZ53_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30472.1"
                     /translation="MSTPRQSPPGPRPDPMGPDPDPSPDPDPIPVPSPDPEPVPEEPA
                     HGDYVAGAAAPQISANAAAPQIDANAAAPQIGANAAAPQIGANAAAPQIGSNAAAAPR
                     PVDDSSIAPLAAARTRRPRIALRFFASAVAAGGAIAACYNDVPGPQGPLPAPVREVRP
                     QGPTPGPILPTPHLAPDLADGGMPVPTPPVPASQTSTSSARLTIPAGAPLDRAPSDRT
                     TTDRTTTDRTTSERTTSNRATSNRTTSGAHVADADRRSDPPPPTQPPPQQPPPQQPPP
                     SDAGVVDSTADLPAELPDAGPLATPDAGQPLRH"
     gene            3984..4634
                     /locus_tag="VMZ53_18315"
     CDS             3984..4634
                     /locus_tag="VMZ53_18315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS30473.1"
                     /translation="MIVWWRECRFIRVNLDSSRRKPAAVRALKTQLREAVRTQILDAA
                     EELIAARGLHGAALAQIAKKAGVAVGTLYNYFADRDAMIRALFESRRAALRPKLLAVI
                     GMGQDLKFEARLQQFVRGVLEVFDEHRRFLKVSTETEHYKISPSTIAADLSAALEMMV
                     AAGLAERAIDPKKAPLLVPMIAGTLRSIVLKRVVDGQPFAADADVMVRLFLDGARR"
     gene            4649..6274
                     /locus_tag="VMZ53_18320"
     CDS             4649..6274
                     /locus_tag="VMZ53_18320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HUS30474.1"
                     /translation="MAKEADAKPQPVGGAPREVGKWTVAISIAFGSLMATIDTSIVNV
                     ALPSIRGELGASLQEITWISTGYMIAMVLVMPLTGFLGSFFGQKRVYLASLILFTVGS
                     LLCGTARSLEMLVLWRIVQGLGGGALQPTQQAILRQTFPPAEQGMAMAIFSMVIMVGP
                     AVGPVLGGYITDNASWPWIFYVNLPVGLLGIYLTWRNVHEPADVLAANRARAEVAKKN
                     LDYAGLILMTVMIASMMYVFEEGASEDWFVSTKIQIATFVTGISLVAFIIRELTAASP
                     VVDLRMFRDPTFASGTAIGSVVFGMLMGSMLLLPVFTQELMHYSATQSGIVLIPRTLA
                     MMVASPFVGRLYNKTSPAILCAFGMLMTALGSWMMADITLQTSTRGLILPLSITGVGF
                     AFLFVPLTTAALSRVPRHELAGAAGVNSFVRQIGASIGLSVFATLFTQFAQTGKVSLS
                     SHVTELRPEVAAQYAQAKAALMARGMSSDFAGAFVQKMYAGRVALQGTVISFDKTFIL
                     QVIAFLAVLPLLVFLRVPRAPVGEKVHVEISAE"
     gene            6280..7506
                     /locus_tag="VMZ53_18325"
     CDS             6280..7506
                     /locus_tag="VMZ53_18325"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HUS30475.1"
                     /translation="MAAKARRAYTILAIVAVVVAAAWGLHRWWAAGKEKTDDAQIDAD
                     VLPLASRVGGVIKTVHISDHQQVKAGDVLFEIDPADLDVELARAEADLEAAKAQQAAA
                     DAQVSITQSSSAGGLSTANAQLTGAAAAVRSADDMARAAEASVARARADLAQADSELA
                     RTQQLLAKEAVTKRELEHAQQARAVAKAALDSAMAQLDSARETKRLASSRVAEAQGRV
                     TQSSPVDAQVAASEASRKLAAARVRSAEVALQKAKLQRSYTTVVAPHDGTVSKLAAHE
                     GQTVGPGQALVMIVPNETYVVANFKESQVGKMKPGNTVDIEIDALPGEEFEGVVETIS
                     PATGARFSLIPPDNATGNFVKVVQRVPVKIRWRKAPPAGVRAGLSAEVTVHLDDVDLT
                     HVDKPPAKQAQLPSPH"
     gene            7602..8411
                     /locus_tag="VMZ53_18330"
     CDS             7602..8411
                     /locus_tag="VMZ53_18330"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HUS30476.1"
                     /translation="MLLAIICAAIGGWFSILCLSVYAHRSMAHRAVTLGPIPSHVMRF
                     WLWFSTGTATRRWVAVHRKHHVYTDVEGDPHSPYLFGLHRIFFLGYWYYKREANKPEV
                     IEQYGKDCPDDWIERNVYEKHDNLGLILMLIADLLIFGVGPGLVAYGVQIIWVSLWAA
                     GVINGIGHAIGYRNWQTGDTSRNIVPIAFLVSGEELHNNHHRWPRSATFSMKWYEFDI
                     GWALIRTLAAVGQAKDIYVKNRRWRAPKLGDLAVSDVLPDEPVATDSSPVA"
     gene            complement(8386..9270)
                     /locus_tag="VMZ53_18335"
     CDS             complement(8386..9270)
                     /locus_tag="VMZ53_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS30477.1"
                     /translation="MSRVLAVIAACLLLSSGMAYADQIEQNVKELQAKSSKVRLAATL
                     SLSKSKDARAVLGVSGALNDDADPTVRRVAALALEKMVDQKTAEDAKALAFDALDKAA
                     KTDGNAKVKSTAAATLKALASLRKSSSSVATAAGGKPAVFVNIDPATDLSKKAPKDAQ
                     DRVAKIVRKSIESKTGYSTTWPGGALPTSKELTTSKSQAYIVASTVKKIEVTKGTRQT
                     TIACTVAIRVAPWGGKDGGEKWEANKAASASGSAKAMTGNSDSAINSGMRDCLEAVTE
                     DVATRQVVPFIKRLATNP"
     gene            9358..10068
                     /locus_tag="VMZ53_18340"
     CDS             9358..10068
                     /locus_tag="VMZ53_18340"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUS30478.1"
                     /translation="MSIIDSLRVLGAMARVTGPSLVEIVTGRLERINVDERTHWFGRR
                     VVELLDVDLHVSGGERLSTDRAYVYMSNHQSHLDIPILYATLPSRTIRMLGKKELFEI
                     PLWGRGLKAAEFIPVDRSNHARAVQSIDEAMRLIRDGVSIYLAPEGTRSVTGKIGKLK
                     KGGFHMAKETATPIVPVAIKGTIDILPRGGRVMQTGKRVDVTIGDPISVEGRELEDLM
                     PEVSAFFTRHVEGSEIER"
     gene            10148..>11215
                     /locus_tag="VMZ53_18345"
     CDS             10148..>11215
                     /locus_tag="VMZ53_18345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUS30479.1"
                     /translation="MTWVVPLALGGLAILVMVTFTMLSSRKRHHSTVAPATVVGPRIL
                     VHDINDDRCTGCDACVAVCPTNVLDLVANKSRVLRFEDCIQCEACMFACPTEALVMFP
                     EGAVPPPLKVPEMDENFQTAVPGQYLIGEVAGKPLVKNAANLGRAVIEHMLTTGLYPG
                     ALGQMDNTVDVAIVGSGPGGLSAALTCIQKGLSYVVLEKEQEIASTIARYPKGKLVMA
                     EPYDTQNVSFLPVFDSSKEQLIPIWKELLERVRVRVNQGESVETVQKLQDGSFEIRST
                     VAAYRAQRVVLSIGTRGKPRTLQVPGENLPKVFSLLDDPDMWAGRSVLVVGGGDSACE
                     AALALSDAGAKVMISYRGKGFN"
BASE COUNT         1821 a         3539 c         3792 g         2063 t
ORIGIN      
        1 cagcatcgcc gcggccgccg ccgccgatac cagcatcgcc gcgaccaccg ccgccaccgc
       61 cgccgccgat accagcatcg ccgcggccgc cgccgccgat accagcatcg ccgcgaccgc
      121 cgccaccgcc gccaccgcca ataccggcat cgccgcgacc actgccgacg cctgcatcat
      181 tgcgaggctg ggtcggcggc gcgacgccgg catcagcgcg cgtctgatac ggatccgccg
      241 tttcgccgaa tgcatcagcg acagacggcg tcgagatcat tgggcggttg acgctcgcac
      301 tatctcgcgc gcggggcgct acttcagatg acaggccgcg cagcgtgctt tgaggggtcg
      361 tgcccgtgcc ggtgcccgtt gggcccgtgc ccgtcgtgcc cgtgttcggc gcaccttgca
      421 ccggttcgcc accgcttggc ggcatggggt tcggcgcagg cgtgctcgat ggagcgttcg
      481 gcgacgcggg cgtcggcgca ggaatcgacg gattcgtggg cgtgccgcca ttggtgggcg
      541 cgctgccatt cgtgggcgcg ctgccattcg tgggcgagct gccattcgtg ggcgtgccgc
      601 cgttcgcgcc gggcgccgtc gtcgcgccat tgacggcacc gctcgacaat gagtgcacgg
      661 gaccaacttg ctgctcgggc gcttccgccg catcgggcgg cgcgacgctc ggatccgacg
      721 gatattcggc cgtcgactga ccgagcggcg cctgcgcgac agtcgtagat tcgggcgagc
      781 gctggtgcga cgacgaagag cacgcagcgc cgacgccgcc cgcgacgaga agcgcggcga
      841 gaaccgtacg aagagagttg agcttggtcc gcatggcagc caatgcgatt tgcgaagccc
      901 atgcccgtcg acgagcctgg cgatgtgcgg gcgatgaaca gtcagtacgt gtcctcggcg
      961 cacgtcgcgg caacgaagcg cgatgtgggt gctggtacgc aactcgcaaa caatctcgaa
     1021 catgccggca cgcgcaatcg atacagcgac tatcgcgttc ggcctggtct cgatcccgat
     1081 caagatctac tcgacctcag aaccgaccca cgagattcac tttcatctga tccatgaagg
     1141 atgtggtgag cgggtcaagc agcagtacac ctgcccgcgg cacggcgtcg tcgagcgcag
     1201 cgagatgtcg aagggcttcg agctgacgaa gggcaacttc gtcgagctct ccaaggaaga
     1261 gctcaaggcg ctcgaagcgg tcgcgagcga tgaagtctcc atccgcgagt tcattcccgc
     1321 cgtcgcgatc gatcccatct acgtcgatcg cacgtacttc ctcggccccg gcaaaggcgg
     1381 cgagcgcgcg taccgcctgc tccgcgatgc actcgaagct gcagagctcg tcggcatcgc
     1441 tgcctacgct gcgcgcggca aagaatacgt cgtgatggtc cgcccattcg aagacggcct
     1501 cgcgatgcac cagcttcgct atcccgacga gatcaagagc tggagtgaag tgcccgtcgg
     1561 ccgcctaccg aaggccgcgc cggccgagct ccaactcgcc ggccagatca tctcgcagct
     1621 tcgtcacgac acgttcgacc cgacgcagta ctccgacgaa gtcaaagacc gcgttcgcaa
     1681 gctgattgcg aagaaggcca aaggcggcga aatcaccgcg ccgcccgaag cgccgaagcc
     1741 ggtcgtcacg gatctcatgg aggccctcaa ggcctcgctc tcgggagagg gagccacaaa
     1801 gccggcggcg gccggctcgg catcccgctc tcgcaagaat ggccacgccc gcaacggcaa
     1861 cggcgccacc aagtcgacca ctcatgctcg tagcagcacg acgaagtcgg cccatggcgc
     1921 acgcggcggc ggaagcaaag cgacatccga atcgtcgggt cgctcgcgtg gtcacgcgca
     1981 cgccaagacg cgcgcaacgc gacctgcagc acgcccccat cgtgctccaa ctcgcgctgc
     2041 acgcagtcag tcgaagcgtg cgcatcgttg acaattgaaa cgcctgcccg cggcgctgcg
     2101 tatgggaggt cgacgcagcg ccgctggccg gcaccttttc gccactcgac gactccgtgt
     2161 atcggaggtt cgcgcggagt tgaggagtgg caccttttcg ccgcttgcgg cgctgtgttc
     2221 gggaggtcga cacagcgcgc gagcggcacc ttttcgccag ctgcggtgta gcgttcggga
     2281 ggtcgacgcg gcgccgcagc tggccctttt cgtcgcgcat ggcgcggctc gttcccgctc
     2341 gcgcgttgct cgtatcgcga tgcccacctc gctacgagat cccggatcag acgaggtgtc
     2401 ttttcggatc ccgtcatgac gatgtactga cgactaaccg cgcgattctt cgtcaccgtt
     2461 cccgggtgat cgcgcagccg cacgaaacgc ggcgagcgtg ttcctcgatg ttccacttcg
     2521 cggcgcttca cggcatcgct gcgctcgagt ggttcgctag gcacgattcg cttcacgtcg
     2581 ctcgagggcg tcgttgcgct cgatgtggtc agctagaacg attcacttca cgtcgctcga
     2641 gggcttcgtt gcgctcgatg tggtcggcta ggacgatacg cttcacgtcg ctcgagggct
     2701 tcgttgcgct cgatgtggtc gctagaacga ttcgcttcac gtcgctcgag ggcttcgttg
     2761 cgctcgatgt ggtcggctag gacgattcgc tcacgtcgct cgagggcttc gttgcgctcg
     2821 agacgctccg ctccgctcga cgtggttcgg tgcgtcgacg agctctcgcg cacttgaacg
     2881 tcatgacggc gtcatgacgg gtctccgaaa gacacaccgc gatcgtaggg atctgctcgt
     2941 ggacgcgcgc gtgcgagcga agcgtccggc atgcgttatc ggcgaacgcg atttgcgcgt
     3001 gctgcgcttg cgcgttagtg gcgcagcggt tggccggcgt cgggcgtcgc aagcgggccg
     3061 gcgtcgggca gctcggccgg aagatcagct gtgctatcga ccacgcctgc gtcggatggc
     3121 ggtggctgct ggggcggtgg ctgttgcggc ggcggttgcg ttggcggcgg cggatctgat
     3181 cggcggtcgg catcggcgac atgcgcgcca ctcgtcgtgc gattgctcgt cgcgcgatta
     3241 ctcgtcgtgc gttcgctcgt cgtgcgatcg gtcgtcgtgc gatcggtcgt cgtgcgatca
     3301 cttggggcgc ggtcgagcgg cgcgccggcg ggaattgtta ggcgcgcgga gctcgtcgat
     3361 gtctgcgacg cggggacggg cggcgtcggg accggcattc cgccgtcggc gaggtccggc
     3421 gcgaggtgcg gcgtggggag gatcgggccg ggcgttgggc cctgcggccg gacttcgcgc
     3481 accggcgccg gcagcggacc ttgcgggccg ggcacgtcgt tgtagcaagc cgcgatcgcg
     3541 ccgccggccg cgactgcgct ggcgaagaaa cgaagtgcaa ttcgcggccg tcgagtgcga
     3601 gcagcggcga gaggcgcaat cgaactgtca tcgacgggtc gtggcgcagc ggcggcattc
     3661 gaaccgattt gcggcgcggc ggcattcgca ccgatttgcg gcgcggcggc attcgcaccg
     3721 atttgcggcg cggcggcatt cgcgtcgatc tgcggcgcgg cggcattcgc actgatttgc
     3781 ggcgcggcgg caccggcaac gtagtcgccg tgggcgggct cttcggggac gggttcggga
     3841 tctggacttg ggacggggat cgggtcggga tccggcgacg ggtccgggtc ggggcccatt
     3901 gggtcggggc gcggaccggg cggggattgg cgcggagtgc tcatggaatc tccgcagtta
     3961 ggtggtgcga ctcgcgtgcc agcttgatcg tttggtggcg tgaatgtaga ttcattcgcg
     4021 tgaatctgga ttcatccaga cggaagccgg cggcggtgcg agcgctgaag acacagctgc
     4081 gcgaggctgt tcgtacgcag atcttggatg cggcggagga gctgattgct gcgcgtgggc
     4141 tgcatggcgc ggcgctggcg cagattgcga agaaggcggg cgtggccgtg gggacgctct
     4201 acaactactt cgcggatcgc gacgcgatga ttcgcgcgtt gttcgagtca cggcgcgcgg
     4261 cgttgaggcc gaagttgttg gcggtgatcg ggatggggca ggacctgaag ttcgaggcgc
     4321 ggctgcagca gtttgtgcgc ggggtcctcg aggtcttcga tgagcaccgg cggtttctga
     4381 aggtctcgac ggagaccgag cactacaaga tttcgccatc tacgatcgcg gcggatttga
     4441 gtgcggcatt ggagatgatg gtggccgcgg gactcgcgga gcgcgcgatc gatccgaaga
     4501 aggcgcctct cctcgttcct atgattgcgg ggacgttgcg ctcgatcgtg ctgaagcgcg
     4561 tcgtcgatgg gcagcccttt gcagccgatg cggatgtgat ggtgcggttg ttcctcgacg
     4621 gagcgcggcg gtagccatca tggaagcggt ggccaaagaa gcggacgcga agccgcaacc
     4681 ggtcggaggg gcgccgcgcg aggtcggcaa gtggaccgtc gcgatctcga tcgcgttcgg
     4741 ctcgttgatg gcgacgatcg atacgtcgat cgtgaatgtc gcgttgccgt cgattcgcgg
     4801 tgagctcggc gcgagcctgc aagagatcac gtggatctcg acggggtaca tgatcgcgat
     4861 ggtgctggtc atgccgctga ctggcttcct cggatcgttc tttggccaga agcgcgtcta
     4921 tttggcgtcg ctgatcctgt tcaccgtcgg gtcgctgctg tgtgggaccg cgcgctcgct
     4981 cgagatgctc gtgctgtggc gcatcgttca ggggttgggc ggcggtgcgc tgcagccgac
     5041 gcagcaggcg attttgcggc agacgtttcc gccggccgag cagggcatgg cgatggcgat
     5101 cttttcgatg gtgatcatgg tcgggccggc cgtggggccc gtgctcggcg gatacatcac
     5161 cgacaacgcg tcgtggccgt ggatttttta cgtgaacctg cccgtgggtc tgctcggcat
     5221 ctatttgacg tggcgcaacg ttcacgagcc cgcggacgtg ctcgcggcga atcgcgcgcg
     5281 tgcggaagtt gcgaagaaga acctcgacta cgccgggctg atcctgatga ccgtcatgat
     5341 cgcctcgatg atgtacgtgt tcgaggaggg cgcgtcagag gattggttcg tctcgacgaa
     5401 gatccagatc gcgacgttcg tgaccggcat ctcgctcgtc gcgttcatca ttcgcgagct
     5461 gacggcggcg tcgccggtcg tggacctgcg catgttccgc gacccgacgt tcgcgagcgg
     5521 tacggcgatc ggctcggtcg tgttcggcat gctgatgggc tcgatgttgc tgctgccggt
     5581 gttcacgcaa gagctcatgc actacagcgc cacgcagtcc ggcatcgtgc tgattccgcg
     5641 gacgctcgcg atgatggtcg cgtcaccgtt cgtcggccgg ctctacaaca agacgtcgcc
     5701 ggcgattctg tgtgcctttg gcatgctcat gaccgcgctc ggcagctgga tgatggcgga
     5761 catcacgctc cagacatcga cgcgcggcct catcctgccg ctctcgatca cgggcgtcgg
     5821 ctttgcgttc ttgttcgtgc cgctcacgac ggcggcactc tcgcgcgtac cgcgtcacga
     5881 gcttgcggga gcggccggcg tgaacagctt cgtacggcag atcggtgcgt cgatcgggct
     5941 ttcggtgttt gcgacgctgt tcacgcagtt cgcgcagacc ggcaaagtga gcttgtcatc
     6001 gcacgtgacc gagctgcggc cggaggttgc ggcgcagtat gcgcaggcga aggcagcgct
     6061 gatggcgcgt ggcatgtcga gcgacttcgc cggcgcgttc gttcagaaga tgtacgcagg
     6121 tcgcgtggct ctgcagggca ccgtgatcag tttcgacaag acgttcatcc tgcaggtcat
     6181 cgcgtttcta gccgtgctcc cgctcctcgt cttcttgcgc gttcctcgcg caccggttgg
     6241 cgagaaggtg cacgtcgaaa tcagtgcgga gtaaatgtca tggcagctaa agcgcgtcgc
     6301 gcatacacca tcctcgccat cgtcgctgtt gtcgtcgcgg ctgcgtgggg cttgcaccgc
     6361 tggtgggccg ccggcaagga gaaaaccgac gacgcacaaa tcgacgccga cgtactgcca
     6421 ctcgcgtcgc gcgtcggcgg cgtgatcaag accgttcaca tcagcgatca ccagcaggtg
     6481 aaagcaggcg acgtcctgtt cgagatcgat cctgcggatc tcgatgtcga gctcgcccga
     6541 gccgaagcgg atctcgaggc tgcgaaggcg cagcaggcag ccgccgatgc gcaggtgtcg
     6601 atcacgcaat cgtcgtcggc cggcgggctg tccaccgcga acgcgcagct gaccggtgcg
     6661 gcagccgcgg tgcgcagtgc cgatgacatg gcccgcgcgg cggaggcatc ggttgctcgt
     6721 gcgcgtgcgg atctcgcgca agccgacagc gagctcgcac ggacgcagca gttgctcgcg
     6781 aaagaagccg tcacgaagcg cgagctcgaa cacgcgcagc aagcacgcgc cgtcgcgaag
     6841 gccgcgctcg actccgcaat ggcgcagctc gacagcgcgc gcgagaccaa gcgccttgca
     6901 tcgtcgcgcg tcgccgaagc gcagggccgc gtcacgcagt cgagcccagt agatgcgcaa
     6961 gtggcggcgt cggaagcgtc gcgtaagctc gccgccgcgc gcgtgcgttc cgccgaggtc
     7021 gcgctgcaaa aggcgaagct ccagcgctcg tacacgacgg ttgtcgcgcc gcacgacggc
     7081 accgtcagca agcttgccgc gcatgaaggc caaacggtcg gtcccgggca ggcgctcgtc
     7141 atgatcgtcc cgaacgagac gtacgtcgtc gcgaacttca aggagtcgca agtcggcaag
     7201 atgaagcccg gcaacaccgt cgacatcgag atcgatgcgc tacccggcga agaattcgag
     7261 ggcgttgtcg agacgatttc gcccgcgacc ggcgcgcgct tctctctgat cccgccagac
     7321 aacgcgaccg gcaacttcgt caaagtcgtg cagcgcgtgc ccgtgaagat tcgctggcgc
     7381 aaggcgccgc ccgccggagt tcgtgcggga ctttcggcgg aggtcacggt gcatctggac
     7441 gatgtcgacc tcacgcacgt cgacaagccg ccggctaaac aagcgcagct cccgtcgccg
     7501 cattgattga cgcggtaagc cgcgcgtcat cgcacagtca tgcgttgaac atgacgaagc
     7561 cgtgacggtg ctctcgacgg tgcggcgata aacaccaggt agtgctgctc gccattattt
     7621 gcgccgcgat cggcggttgg ttttcgatcc tgtgtctcag cgtgtacgcg catcgttcga
     7681 tggcgcatcg cgcggtgact ctcggtccga ttccatctca cgtgatgcgc ttctggctct
     7741 ggttctcgac gggcacggcg acgcgccgct gggttgccgt ccatcgcaag caccacgtct
     7801 acaccgacgt cgaaggcgat ccgcactcgc cgtacttgtt cggcctgcac cgcatcttct
     7861 ttctcggcta ctggtactac aagcgcgagg cgaacaagcc cgaggtcatc gagcagtacg
     7921 gcaaggactg tcctgacgat tggatcgaac gcaacgtcta cgagaagcac gacaacctcg
     7981 gcctcatcct catgctcatc gccgacctgc tgatcttcgg cgtcggcccg ggcctcgtcg
     8041 cgtacggcgt gcagatcatc tgggtctcgc tctgggccgc tggcgtcatc aatggcatcg
     8101 gtcacgcgat cggctatcgc aactggcaga ccggcgacac gagccgcaac atcgtgccga
     8161 tcgcgtttct cgtgtcgggc gaagagctgc acaacaacca ccatcgctgg ccgcggtccg
     8221 cgacgttctc gatgaagtgg tacgagttcg atatcggctg ggcgctgatt cgcacgctcg
     8281 ccgccgtcgg gcaggcgaaa gatatctacg tgaagaaccg ccgctggcgc gcgccgaagc
     8341 tcggagacct cgccgtgtcc gacgtgctgc cggacgagcc ggtcgctacg gattcgtcgc
     8401 cagtcgcttg atgaacggca cgacctgccg cgtggcgacg tcctcggtga cggcctcgag
     8461 gcagtcgcgc atgccgctgt tgatcgcgct gtcggagttg ccggtcatcg ccttcgcgga
     8521 gcccgatgcc gacgcggcct tgttcgcttc ccacttctcg ccgccgtctt tgccgcccca
     8581 cggcgcgacg cgaatggcca cggtgcacgc gatcgttgtc tgacgtgttc ccttcgtcac
     8641 ttcgatcttc ttcacggtcg acgcgacgat ataggcctgc gacttgctcg tcgtgagctc
     8701 cttgctcgtc ggcagtgcgc cgcccggcca cgtcgtcgag taacccgtct tcgactcgat
     8761 gctcttgcgc acgatcttcg cgacgcgatc ctgcgcgtct ttgggcgcct tcttcgatag
     8821 gtccgtcgcc ggatcgatgt tgacgaacac ggcgggcttg ccgcctgccg ccgtcgcgac
     8881 cgacgagctc gacttgcgaa gcgatgcgag cgccttgagc gttgcggctg cggtcgattt
     8941 cactttcgcg ttgccgtcgg tcttcgcggc cttgtcgagt gcgtcgaacg cgagcgcctt
     9001 tgcatcctcc gccgtctttt gatcgaccat cttctcgagc gcgagtgcgg cgacgcgacg
     9061 aaccgtcgga tcagcatcgt cgttgagcgc gcccgagacg ccgagaaccg cgcgtgcgtc
     9121 cttgctcttc gacagcgaga gcgttgcggc gagtcgaacc ttcgacgatt tcgcctgcag
     9181 ctctttcacg ttctgctcga tttggtcggc gtaagccatg ccgctcgaga gcagcaagca
     9241 agcggcgata accgcaagca cgcgcgacat cgcctcaaca ttaacacgcg agaaattagg
     9301 tgagcaggcc cacacctccc acctcggcgg cggatgtcgc ggggtaggat gtgccgcgtg
     9361 agcatcatcg atagcctccg tgtgctcggc gcgatggcac gcgtcaccgg cccatcactc
     9421 gtcgagatcg tcaccgggcg gctcgagcgc atcaacgtcg acgagcgcac gcattggttc
     9481 ggccgccgcg ttgtcgagct gctggatgtc gatctgcatg tgagtggagg cgagcgcctg
     9541 agcacggatc gcgcgtacgt ctacatgtcg aaccatcaga gccatctcga catcccgatc
     9601 ctctatgcga ccttgccctc gcgcacgatc cggatgctcg ggaagaagga gctgttcgag
     9661 atcccactct ggggacgcgg gctcaaagcg gcggagttca tccctgtcga tcgcagcaat
     9721 cacgcgcgcg cggtgcaatc gatcgatgaa gcgatgcgcc tcattcgcga tggcgtgagc
     9781 atctatctcg cgcctgaagg cacgcgctcg gtgaccggca agatcggcaa gctgaagaag
     9841 ggcggctttc acatggcgaa ggagaccgcc acgccgatcg tccccgtcgc gatcaaaggg
     9901 accatcgata tcctgccgcg aggtgggaga gtgatgcaga ccgggaaacg cgtagacgtg
     9961 acgatcggtg acccgatttc cgtcgaggga cgggagctcg aggacctaat gcccgaggtt
    10021 tcggccttct tcacccggca tgtcgaaggc tcagagatcg agaggtagct gagactacgg
    10081 agttttctgg cgacacgcgc atgcagaaga gcgggtcacg gacctcttta tagggtatct
    10141 tgatccggtg acctgggtcg tgccgttggc gctcgggggc ctcgccatcc tcgtgatggt
    10201 gacgttcacc atgttgtcgt ctcggaagcg acaccactcg actgttgcgc ctgcaacggt
    10261 ggtcggtcct cgcatcctcg ttcacgacat caatgatgat cgctgcaccg ggtgcgatgc
    10321 gtgcgtcgcc gtgtgtccga cgaacgtgct tgacctcgtc gcgaacaaga gccgcgtgct
    10381 ccggttcgaa gactgcatcc agtgcgaggc atgcatgttc gcgtgcccga ccgaagcgct
    10441 cgtcatgttc cccgaaggcg ccgtgccgcc gccgctcaaa gttcccgaga tggacgagaa
    10501 ctttcagacc gcggtgccgg gccagtactt gatcggcgag gtcgccggca agccgctcgt
    10561 caagaacgct gcgaacctcg gtcgcgcggt gatcgagcac atgctgacga cgggcctcta
    10621 tccaggcgcg ctcggccaga tggacaacac cgtcgacgtc gcgatcgtgg gctcggggcc
    10681 cggcggtctg tcggccgcgc tcacgtgcat tcagaagggc ctgtcctacg tcgtgctcga
    10741 gaaggagcag gagatcgcgt cgacgatcgc gcggtatccc aagggcaagc tcgtcatggc
    10801 cgagccctac gacacgcaga acgtgtcgtt cctcccggta ttcgactcgt ccaaagaaca
    10861 gctcattccg atctggaagg agctactcga gcgcgtgcgc gtgcgcgtca atcaaggcga
    10921 gtcggtcgag accgtgcaga agctccagga tggcagcttc gagatccgca gcaccgtcgc
    10981 ggcgtatcgt gcgcagcgcg tcgtgctctc gatcggtacg cgcggcaagc cgcgtacgct
    11041 gcaggttccc ggcgagaacc tgcccaaggt gtttagcctg ctcgacgacc ccgacatgtg
    11101 ggccggtcgc agcgtgctcg tcgtcggtgg cggtgactcg gcttgcgaag ccgcgctcgc
    11161 cctctccgat gcgggcgcca aggtcatgat cagctaccgc ggcaaaggct tcaac
//
LOCUS       DATLBZ010000355        11064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9900673, whole genome shotgun sequence.
ACCESSION   DATLBZ010000355 DATLBZ010000000
VERSION     DATLBZ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9900673"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1085)
                     /locus_tag="VMZ53_18350"
     CDS             complement(261..1085)
                     /locus_tag="VMZ53_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30480.1"
                     /translation="MNRALVVGALIATACCGPKGSKPPSNDLTVQQIVDNTAKARGEL
                     TSFRGDSTMDYWLSGQRAKGEVLVMGTTGKKVRFAALSPAGGATLAEMACDGTNFVYV
                     DYQNNCSLSGPCDASSIATFFGIALEPDDFLHLALGTPPIVDGAKGTSTWDSSRGVWK
                     AELSGTEGSQSLAIDNKEGHWDVVETELKGTDGKPRWSVKNTDFDKVEGSGDRRVPKK
                     TQFKSPVGNQDLLVQWDDLVVNQKIDPQKFTLTAPAGLPTCGGQPTQMAPSAGAKP"
     gene            complement(1115..1744)
                     /locus_tag="VMZ53_18355"
     CDS             complement(1115..1744)
                     /locus_tag="VMZ53_18355"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS30481.1"
                     /translation="MTAAVASASLSDTDLIARVVGGSREDFEALVRRHNQRLYRAARA
                     IVKSDDEAEDVVQQTWLEVFRHLPQFRGDAQFTTWATRIAVNAALAVARKRPVIAEVS
                     DVDAAHDEAPDQTVQREELGRVLEECLQRLPQGNREVMVLRDVLELDTAETAAMLGLS
                     EEAVRVRLHRARAAVAADLTERMIDKVYSFDGERCNRLTAAVMNALTDS"
     gene            complement(1775..2227)
                     /locus_tag="VMZ53_18360"
     CDS             complement(1775..2227)
                     /locus_tag="VMZ53_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30482.1"
                     /translation="MALALGTRRSTIAAVTSALAAMALAAAVAGRSCQVTKPGPEVTV
                     RDLLQAAKQGNRDLVYELLTPGTRARLQAEANRATDLVGAATRYTAKDLISIGATGEA
                     PTDITVIEERGGRAVVEIVSPAGRSRLTLLEIEGRWRVDLAAYGAPSL"
     gene            2393..3628
                     /locus_tag="VMZ53_18365"
     CDS             2393..3628
                     /locus_tag="VMZ53_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HUS30483.1"
                     /translation="MNFRRVAKLIGVAGLGFALQACVVRETRPVNPGYGYNAGYRANV
                     TVGTPSAYYVSSMPPQPLYESMSASPGYGYSWIDGYWHWNGYEWVWVSGRWERQQEGY
                     VYISPYYDYSGGSYVYQPGYWTSNDRVPQGWQVRDHREGRPTSYVPPAGWHANPPPVA
                     GGGGGVRPPPTGGGGGWNPPPTGSTPPSGGSPPPSGGGWTPPGHGGTPPGLGQAPPAQ
                     PGNNGNGNGWGPGGRPIPPGVGQAPPAQPGNNGNGNGWGPGGRPVPPGVGQAPPVTQD
                     PGRGGPRPGLGQAPPVTQDPGRGGPPGPRPGLGDAPPVTQQPSGPQHPPPPNGAGQPP
                     SGNQGPPGPRPMPPGRVNSGHAPGPSAPPAQPAQPAPSQPARPVGPPPQSHAPSAPAQ
                     PSGPPPSSGPPAMKGRGHR"
     gene            complement(3729..4808)
                     /locus_tag="VMZ53_18370"
     CDS             complement(3729..4808)
                     /locus_tag="VMZ53_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUS30484.1"
                     /translation="MDRSAFHKLLAFGIERGVSDIHFEVGYPPHYRLHGELLGAIKVP
                     ALTAADTEAIARMILEDRSITVDFTRRFAEIDVSYSLAQRGRFRSSIFRQRGAVGIVM
                     RLIPIQILSIEQLNLPPVLAEIADARRGLVLITGATGNGKTTTMAAMLRYINETRHAH
                     IVTIEDPIEFIHEPQKCMIIQREIGADTESFRDAMTAAMRQDPDVILVGEIRDRETAA
                     TALKAAETGHLVISAIHTPDAVSTVQRYVGLFETDAQDVIRDRLGDCLQAVVSLRLLA
                     SKDGRRRLPATEILRVTRTIRECIRSSRLSDIPELIRKGRDLYSMQLFDQHLIDLVNA
                     GLVSMETAIYASSNPEEFERSLRVE"
     gene            complement(4847..7612)
                     /locus_tag="VMZ53_18375"
     CDS             complement(4847..7612)
                     /locus_tag="VMZ53_18375"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUS30485.1"
                     /translation="MAVPEGQEAAQEDPSKPVVICVDDDQNNLNALGRVLRARCTVML
                     ASSGQQALELLEQTADVAAVLADLHMPGLAGAELLARVAQLRPSCRRAVVTGYPESEE
                     LISAINAGHLHYVITKPWKLTDLLQVIDQLIHTFKLEKDNNRLLDELRNVNTQLREHE
                     RILEAQLSERGREISAATEQLAQMGNQLDALTLRDPLTGLYTHRAFQERLREEVARAN
                     RYSQPMSLLIADIDQFASVNYDLGYQAGDDILRKIATILQEDESPERVRTSDVVARYS
                     GEEFVLLLPETPKPGALTKAARLRDAVAVQAMPGGRKLTLSIGVACLPEDAADPEGLL
                     TAAEAALRGAKRGGPGRIHFFSSGDAGPTPNRSRPFGRVREAEIDRFRPYQERMNEVT
                     AILNRDRSLSCLLVDLSRLHRVELDLGVAHHSEIYDAAAAVLDRLRGAVLDPADLICR
                     SGDGNGYLVILAPRGAHRIDLEQLANSVEKAVEEALSPMVSEVLREQPRITVGAARVL
                     GNSLIRPERLTARLVSDAVDNARNLRERKAHRDRTTLQDVILGDGLTSVYQPIVDLGT
                     GDIFAFEALSRGPRTTPFESPATLFAIADEVDLTVELDRACFRGALRNAMTLEPVHRL
                     FVNLLPMSFYDSAFIEVEVGNLLSAAGLTPANIVFEITERLAIENFASFKRALANYTA
                     MGFGVAIDDVGTRHSNLETVMSLRPHFIKISDVLVRGIARSTVKREMLRSLRHIAETI
                     DAVMIAEGIEHVEDVIALRELGLRYGQGYYMARPGPPFPSLPDYVRAELRSVATTQAN
                     GPQPKISSSAEVDDEEDDDDRDLPIAKAQPQKSSAIPRNVFGRAEDEITSDFALPGSE
                     RRPRASDPPASEQPTQPIPAPAPAAASPWQPLSDDEATGEALLSSLKRAENASEGEPG
                     PGGPGLN"
     gene            complement(7711..8805)
                     /locus_tag="VMZ53_18380"
     CDS             complement(7711..8805)
                     /locus_tag="VMZ53_18380"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HUS30486.1"
                     /translation="MLRALMVATMILLGSSTAHAHQTSVKYIDLVLYPMPSGVTGYVH
                     VTVKLAPGDVTEPMGLAADAKPSVADAVRRPEVASYVQHWFEIRDCEPPGVPEAKAID
                     DGYLEVRWRVGCEKVVPLQLDFARFFALDQRHEAIVRLASPGRKTIQTIVRASEQTVA
                     LRAGESPSLLAWVRTGMDHIYDGTDHILFVISLLLVVMLARGADAAWELRGFVRTLRS
                     TAAVITAFTVAHSISLIAASLGWVHLPSAFVEAVIALSILYTAVEDIVKPDVRWRFFL
                     TFGFGLVHGLGFAGTLSELLPPRDVVVPLLCFNVGVEIGQLTIVAVVLPALYLVARGL
                     GAPAYRRYALPVVAGAFALVALQMFVARVT"
     gene            complement(8840..9775)
                     /locus_tag="VMZ53_18385"
     CDS             complement(8840..9775)
                     /locus_tag="VMZ53_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30487.1"
                     /translation="MRTSVVRGLALMVVAACSSPTSPAPTAPVETHTPVDERSRDWLP
                     IVDSHVHLAYYPVADQLAKHGVSAAVDLAAPESALGTKYPIKVIQAGPMLTHPNGYPL
                     DAWGSDGYGIGCDSPSCVTDAIDRLQQEGARIIKIALDDDGLDERLARYAAIHAHQRH
                     MLVVVHALSDESARFAAEIDADALAHTPVEALSEATITAWQVRVRKARPIDGMAVTRD
                     QRPKRAVITTLAAFGGSPIAIDNLRRLRAAGLVVLYGTDLGNLRVDGLSTDEIELMKS
                     AGMTDADVRTSMTSAPWSFWRFDELGPIPGKPAEQ"
     gene            complement(9792..10379)
                     /locus_tag="VMZ53_18390"
     CDS             complement(9792..10379)
                     /locus_tag="VMZ53_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HUS30488.1"
                     /translation="MRPAVPSRAFVGLTVISLSAACGASLGGDDQTNFEGVDAKVYRD
                     AGIDAPPDARVCVGGTPAMLAPDGSCFGLITTPQTYVQAKAACAALAPGGHLAYIKSA
                     ALDTLAESFVGTANTWIGADDRTTEGTFLWADGSALTFTNWHAGEPNSGGVGATYQED
                     CVIIAGARVDKQWDDRPCDATEVATSGMFASLCQY"
     gene            10418..>11064
                     /locus_tag="VMZ53_18395"
     CDS             10418..>11064
                     /locus_tag="VMZ53_18395"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HUS30489.1"
                     /translation="MSRQAPLVFIMAAGLGTRMKSDTSKVLHHIAGRPMLHWVVTAAR
                     TAGAERVVAILGHQNEAVKASLDGSFGVGSVDVALQPEQRGTGHAVQCALPTVQNESE
                     DRIVVILSGDAPLLQTERISELVRACAEAPGGMALLSTRPDRDMPYGRLVRDAKGVLE
                     KIVEHPDATPEQRQIKDTNAGFYAIRLGHLRRDLQDLRANNAKGELYLTDLVAAA"
BASE COUNT         1889 a         3675 c         3612 g         1888 t
ORIGIN      
        1 ggcaatgcga gccgcgacag atcgatccgg agccgaaccg gatggccaga gcgcggcgtt
       61 tcctcgcgca ccgtcgatgg ctccaccgac aatcgcatcg gcgactccgc atacgccgcc
      121 ccagacatcg cgaggatgag catcaagctg aatcgcccca cgccacatgt acgaggcgcg
      181 aggtccggcg gatcgttgct cgagacttcg cgagcttaga accgttactt gtttacttgt
      241 gcctgacccc gttccacaag ttagggcttt gcgccggccg acggggccat ctgcgtcggt
      301 tggccgccgc acgtgggcag gcccgcgggc gcggtcagcg tgaacttctg cggatcgatc
      361 ttctggttca cgacgaggtc atcccactgc acgagcaggt cttggttgcc gaccggcgac
      421 ttgaactggg tcttcttcgg cacgcgtcga tcgccgctgc cctcgacctt gtcgaagtcc
      481 gtgttcttca ccgaccagcg cggcttgccg tccgtgccct tgagctcggt ctcgacgaca
      541 tcccagtgcc cctctttatt gtcgatcgcg agcgactgcg atccctcggt gccggagagc
      601 tcggctttcc acacgccgcg cgacgaatcc cacgtcgacg tgcccttcgc gccatcgacg
      661 atcggcggcg tgccgagcgc gagatgcagg aagtcatcgg gctcgagcgc gatgccgaag
      721 aacgtcgcga tcgagctcgc atcgcacggg ccggagagcg agcagttgtt ctggtagtcg
      781 acgtacacga agttcgtgcc atcgcacgcc atctcggcga gcgtcgcgcc acccgccggc
      841 gagagcgcgg cgaagcgaac cttcttgccc gtcgtgccca tcacgagcac ttcgcctttc
      901 gcacgttggc cgctgagcca gtagtccatc gtgctgtcac cgcgaaacga cgtcagctcg
      961 ccgcgcgcct tcgccgtgtt gtcgacgatc tgctgcacgg tgagatcgtt cgaaggcggc
     1021 ttcgatcctt tcggaccgca acacgcagtc gcgatgagtg ctccaacaac caaggctctg
     1081 ttcatgcctg ggtcatagca gagctgcgcg cgcgctacga atcggtcagt gcattcatca
     1141 cggcggccgt caaacgattg caacgttcgc cgtcgaatga ataaaccttg tcgatcatcc
     1201 gctccgtgag atccgcggca accgcggcac gcgcgcgatg cagacgaacc cgcacggctt
     1261 cttccgagag accgagcatt gcggccgtct cggcggtgtc gagctcgagc acgtcgcgca
     1321 gcaccatgac ctcgcggttg ccctgcggca ggcgctgcag acattcttcg agcacgcggc
     1381 cgagctcttc gcgctgcacc gtttgatcgg gcgcttcgtc gtgcgccgcg tcgacatcgc
     1441 tcacctcggc gatcaccggc cgcttgcgcg cgaccgcgag tgccgcgttg acggcgatgc
     1501 gtgttgccca cgtcgtgaac tgcgcgtcgc cacgaaactg cggcaggtgg cgaaacactt
     1561 cgagccacgt ctgctgcacg acgtcttccg cttcgtcgtc cgatttcacg atcgcgcgcg
     1621 cggcgcgata gaggcgctgg ttgtggcgac gcacgagcgc ttcaaaatct tcgcgcgatc
     1681 caccgacgac acgcgcgatg agatcggtat ccgagagcga tgcggaagca acagcggcgg
     1741 tcatgctcgt tggagtgtgt acccccgcgt cgcgttacag gctcggcgcg ccataagctg
     1801 cgagatccac gcgccaccgg ccttcgatct cgagcagtgt gagccgcgag cgtcccgccg
     1861 gcgagacgat ctcgacgact gcgcgaccac cgcgctcttc gatgacggtg atgtccgtcg
     1921 gcgcttctcc ggtcgctccg atcgagatca gatcctttgc ggtgtagcgc gtcgctgcgc
     1981 cgacgagatc cgtcgcgcga tttgcttcgg cttgcagacg cgcgcgagtt ccgggcgtga
     2041 gcagctcgta cacgaggtcg cgattgccct gctttgcggc ctgcagaaga tcacgcacgg
     2101 tgacctcggg gcccggcttc gtgacctggc agctgcgccc agcgactgcg gcggccaagg
     2161 ccatcgcggc gagtgcactc gtgaccgcgg cgatggtcga gcgacgggtg cctaacgcca
     2221 gtgccacggc gcgaacatgc cctggcaagg gggttgcagt cctctggaat cgctagacga
     2281 tgccgagggt tctgttttca tcggcattct ctgtacttgt gagccactgg accgtcaact
     2341 aggatgaatc attggtttgg attgccgtcc aaccacttag gaggctaggg acatgaactt
     2401 tcgacgcgtt gcaaaactga ttggcgtagc cggactcggc ttcgcgttgc aggcgtgtgt
     2461 cgttcgagag acgcgtcccg tgaaccccgg ttacggctac aacgcgggct accgcgcgaa
     2521 cgtcaccgtc ggtactccga gcgcttacta cgtctcatcg atgccgccgc agccgctgta
     2581 tgagtcgatg tcggcgtcgc ccggctacgg ctactcgtgg atcgacggct actggcactg
     2641 gaacggctac gagtgggtgt gggtcagcgg ccgctgggag cgccagcaag aaggctacgt
     2701 ctacatctcg ccgtactacg actacagcgg cggttcgtac gtctatcaac ccggctactg
     2761 gacatcgaac gatcgcgttc cgcagggctg gcaggttcgc gatcatcgcg aaggtcgtcc
     2821 gacgagctac gtcccgcccg cgggatggca cgcgaatcct ccgccggtcg ccggtggtgg
     2881 tggcggcgtt cgtccgcctc cgacgggtgg tggcggtggt tggaatcctc cgccgacggg
     2941 cagcacgcct ccgtcgggtg gatcgcctcc gccgagcggt ggtggttgga cgcctccggg
     3001 ccatggcgga acgccgccgg gccttggtca agcgccgccc gcgcagcccg gcaacaacgg
     3061 caatggaaac ggttggggcc ctggcggtcg tccgattcct cccggtgttg gtcaagcgcc
     3121 gcccgcgcag cccggcaaca acggcaacgg caatggttgg ggccctggcg gtcgcccggt
     3181 tcctccgggt gtcggccaag ctccgccggt cacgcaggat ccaggtcgcg gtggccctcg
     3241 tccgggtctc ggacaagcgc cgcccgtgac gcaagatccg ggtcgtggtg gtcctccagg
     3301 cccacgtccg ggtctcggtg atgcaccgcc cgtgacgcag cagccgtccg gaccgcagca
     3361 tccgccgccg ccgaatggcg caggccaacc gccgagcgga aaccagggtc ctccaggccc
     3421 gcgtccgatg ccgcccggtc gtgtgaactc tggccacgcg ccaggaccgt cggctccgcc
     3481 tgcgcaaccg gcgcagcccg caccgtcgca acctgcacgc ccggtcggtc ctccgccgca
     3541 atcgcacgcg ccgtcggctc ccgcgcagcc gtcgggtcct cctccgtcgt cgggtcctcc
     3601 cgcgatgaag ggccgaggac atcgctgaga cggaaccgtc actgacgcgc acagcgtgag
     3661 tgaacagacg gcgccacctc ggcgccgttt gcattttcgg gcgagaattc ccgaggcgcg
     3721 tggcccaact attcgacgcg cagcgagcgc tcgaactctt cgggattcga gctggcgtag
     3781 atagccgtct ccatcgacac gaggcccgcg ttgacgagat cgatcaggtg ctgatcgaac
     3841 agctgcatcg agtagaggtc gcggcctttg cggatgagct cgggaatgtc gctgaggcgc
     3901 gacgagcgaa tgcactcgcg aatcgtgcgc gtgacacgga ggatctcggt ggcgggcagc
     3961 cggcgacgac cgtccttgct cgcgagcagg cgcagcgaca cgacagcctg caggcaatcg
     4021 ccgaggcgat cgcgaatgac gtcctgcgca tcggtctcga agaggccgac gtagcgctgc
     4081 accgtggaga ccgcatcggg cgtgtggatc gcgctgatga cgaggtgacc tgtctcggcg
     4141 gccttgagcg cggtggcggc tgtctcgcga tcacggatct cgccgacgag gatcacgtcg
     4201 ggatcctggc gcatcgcagc cgtcatcgcg tcgcggaacg actcggtgtc cgcgccgatc
     4261 tcgcgctgga tgatcatgca cttctgcggc tcgtggatga actcgatcgg atcttcgatc
     4321 gtgacgatgt gcgcgtgccg cgtctcgttg atgtagcgga gcatcgccgc catcgtcgtc
     4381 gtcttgccgt taccggtcgc gcccgtgatg aggacgaggc cacggcgagc gtcggcgatc
     4441 tcggcgagca ccggcggcag gttcagctgc tcgatcgaga ggatctggat cgggatgaga
     4501 cgcatcacga tgccgaccgc gccgcgctgc cggaagatcg aagagcggaa gcggcctcgt
     4561 tgcgcgagcg agtacgacac gtcgatctcg gcgaaccgac gcgtgaagtc gaccgtgata
     4621 ctgcggtcct cgaggatcat ccgcgcgatc gcttccgtgt cggcggccgt gagcgccggg
     4681 actttgatcg cgccgagcag ctcgccgtgg agacgatagt gcggcggata gccgacttcg
     4741 aagtggatgt ccgagacgcc gcgctcgatg ccgaacgcga gaagcttgtg aaacgcgcta
     4801 cgatccattg tgacggccag ggaccgggca gccggtcatt gcggcgtcag tttaaacccg
     4861 gtccgcccgg tcctggctcg ccctcggacg cgttttctgc gcgcttcagg ctggatagga
     4921 gcgcttctcc agtcgcttcg tcgtcactca gcggctgcca cggcgacgca gccgcaggag
     4981 ctggcgccgg gatcggctgg gtcggctgct cgctcgccgg cggatccgac gcccgcggac
     5041 ggcgctcgct tcccggcagc gcgaagtccg acgtgatttc gtcctcggcg cggccgaaca
     5101 cattgcgcgg gatcgcgctc gatttctgcg gctgcgcctt cgcgatcggg agatcgcggt
     5161 cgtcatcatc ctcttcgtcg tcgacctccg ccgaggagga gatcttcggc tgcgggccgt
     5221 tcgcctgcgt cgtcgcaacg gaccgcagct ccgcgcggac gtagtccggc agcgacggga
     5281 acggcggtcc cggccgcgcc atgtagtagc cctggccata gcgcaggccg agctcgcgca
     5341 gcgcgatcac gtcttcgacg tgctcgatgc cttccgcgat catcacggcg tcgatcgtct
     5401 cggcgatatg ccggagtgac cgcaacatct cgcgcttgac cgtcgaccgc gcgatgccgc
     5461 gcacaaggac gtccgagatc ttgatgaagt gcggacgcaa cgacatcacc gtctcgaggt
     5521 tcgagtggcg cgtacctacg tcgtcgatcg cgacgccgaa gcccatcgcc gtgtagttcg
     5581 cgagcgcgcg cttgaacgaa gcaaagttct cgatcgccaa acgctcggtg atctcgaaga
     5641 cgatgtttgc gggcgtgagg ccagcggccg agagcaaatt gccgacctcg acctcgatga
     5701 atgccgaatc gtagaacgac atcggcagga ggttgacgaa caggcgatgc accggctcga
     5761 gcgtcatcgc gttgcgaagc gcgccgcgga agcacgcgcg gtcgagctcg acggtcagat
     5821 cgacttcatc ggcgatcgca aacagcgtcg cgggagactc gaacggcgtc gtgcgtgggc
     5881 cgcggctgag cgcctcgaac gcgaagatat cgccggtgcc gaggtcgacg atcggctgat
     5941 acacgctcgt caggccgtcg ccgaggatga cgtcttgcag cgtcgtgcga tcgcggtgcg
     6001 ccttgcgctc gcgcaggttg cgcgcattgt ccaccgcgtc gctcacgaga cgcgcggtca
     6061 accgctccgg gcgaatcagc gagttgccga gcacgcgcgc tgcaccgacg gtaatacgcg
     6121 gctgctcgcg aagcacttcg ctcaccatcg gcgacaacgc ctcttccaca gctttctcga
     6181 cgctgtttgc gagctgctcg agatcgatgc gatgcgcacc gcgtggcgcg aggatgacga
     6241 gatagccgtt gccgtcgcca gagcgacaga tgaggtcagc tggatcgagc accgcgccgc
     6301 gcaagcgatc gagcacggcc gccgcggcgt cgtagatctc ggagtgatgc gcgacgccga
     6361 ggtcgagctc gacgcgatgc aaccgcgaca gatcgacgag caagcatgac agtgagcgat
     6421 cgcgattcaa gatcgcggtc acctcgttca tgcgctcctg atacggccgg aagcgatcga
     6481 tctctgcttc gcgcacgcga ccaaacggcc ggctgcggtt gggcgtaggg cccgcgtcgc
     6541 ccgacgagaa gaagtgaatg cgtccgggcc cgccgcgctt cgcgccacgc agcgccgctt
     6601 cggccgccgt caacaagccc tcgggatccg ctgcatcctc gggcaagcac gcgacgccga
     6661 tcgacagcgt cagcttgcga ccgccgggca tcgcctgcac ggccactgca tcccgcagtc
     6721 gcgccgcctt cgtgagcgca ccgggcttcg gcgtctccgg cagcagcagc acgaactcct
     6781 cgccggagta ccgcgcgacg acgtcggacg tccgcacgcg ctcgggcgac tcgtcctcct
     6841 gcaagatcgt cgcgatcttc cgcagaatat cgtcgccggc ttgatagccg aggtcgtagt
     6901 tcacgctcgc gaactgatcg atatcggcga tcagcagcga catcggctgg ctatagcgat
     6961 tcgcgcgcgc gacttcttca cgcagccgct cctgaaacgc gcggtgcgtg tacagacccg
     7021 tcagcggatc gcgcaacgtg agcgcgtcga gctggttgcc catctgcgcg agctgctccg
     7081 tcgcagcgct aatctcgcgg ccgcgctcgc tcagctgcgc ttccaagatc cgctcgtgct
     7141 cgcgcagctg cgtgttcacg ttccgcagct cgtcgagcaa ccgattgttg tccttctcga
     7201 gcttgaacgt gtgaatgagc tgatcgatga cctgcaacag atccgtcagc ttccacggct
     7261 tcgtgatcac gtaatggaga tgccccgcat tgatcgcgga gatcagctct tcagactccg
     7321 gatagcccgt caccacagcg cgccggcatg acggtcgcag ctgcgcgacg cgcgccaaca
     7381 gctccgcgcc agccaatcca ggcatgtgca gatccgcaag gaccgccgcg acatcggccg
     7441 tctgctccaa cagctcgagc gcttgctggc cgctcgacgc gagcatgacg gtgcagcgcg
     7501 cgcgtagaac gcgaccgagc gcattcaagt tgttctggtc gtcgtcgaca cagatgacaa
     7561 caggcttgct cgggtcctcc tgcgcggctt cttgaccctc ggggactgcc aatgtgattc
     7621 caatatatct cggtcgacca agacgccgga accatgaagg gcgggttagc gcgacacgcg
     7681 ctgctcacgt ccgcctacat cgggatccgc tcaggtcacg cgcgcgacga acatctgcaa
     7741 cgccacgagc gcaaaggctc cagcgaccac gggaagcgcg taacgacggt aggccggcgc
     7801 acctaagccc cgcgcaacca ggtacagcgc cggcagaacc accgcgacga tcgtcagttg
     7861 cccgatctcg acgccgacgt tgaaacacaa gagcggcacc accacgtcgc gcggcggcaa
     7921 gagctcactc agcgtccccg cgaagccgag cccatgaaca agtccgaagc caaacgtcag
     7981 aaaaaacctc cagcgcacgt cgggcttcac gatgtcctcg accgccgtat agagaatcga
     8041 cagcgcgatc accgcctcga cgaacgccga cggcaagtgc acccacccaa gtgacgccgc
     8101 gatcagcgag atcgaatgcg cgacggtaaa cgcggtgatg accgccgcgg tcgatcgcaa
     8161 cgtccgcacg aagccgcgca gctcccacgc tgcatcggca ccgcgcgcca acatcacgac
     8221 aagcagcagc gagatgacga acagaatgtg atcggtgccg tcgtagatgt ggtccatccc
     8281 agtccgcacc cacgcgagca agctcggcga ctcgcccgca cgcagcgcga ccgtttgctc
     8341 gctcgcgcgc acgatcgtct ggatcgtctt tcggcccggt gatgcgagcc gaacgatcgc
     8401 ctcgtgccgc tgatcgagcg cgaagaagcg cgcgaagtcg agctgcagtg ggacgacctt
     8461 ctcgcagccg actctccaac gaacctcgag gtagccgtcg tcgatggcct ttgcttcggg
     8521 tacgcccggc ggctcgcaat cgcgaatctc gaaccagtgc tgcacgtacg acgcgacctc
     8581 cggcctgcgg accgcatctg ccacgctcgg ctttgcatcg gcggcgagcc ccatcggctc
     8641 ggtgacatcg ccgggtgcga gcttcacggt cacgtggacg tatccggtga ctccgctcgg
     8701 catgggatag aggacgaggt cgatgtactt gacgctcgtc tgatgcgcgt gtgccgtcga
     8761 cgatcccaga aggatcatcg tcgcgaccat gagcgcgcgc agcacatcgc ggttctaccg
     8821 cggctcgaca ctcctcgcgc tactgctcgg ccggtttgcc cggaattggc cccagctcgt
     8881 cgaagcgcca aaagctccaa ggcgcactcg tcatcgaggt gcgcacgtca gcgtcggtca
     8941 tgcccgcgct cttcatgagc tctatctcgt ccgtgctcag cccatcgacg cggaggttac
     9001 caaggtccgt gccgtacaga acgacaagcc cagccgcacg aagccggcgc agattgtcta
     9061 tcgcgatcgg gctgccgccg aatgccgcga gtgttgtgat cacggcgcgc ttcggccgct
     9121 ggtctcgcgt cacggccatc ccgtcgatcg gacgcgcctt gcgaacgcgt acttgccacg
     9181 cggtgatggt cgcttccgaa agcgcttcga ccggggtgtg ggcgagcgcg tcggcgtcga
     9241 tctccgctgc gaacctcgcg ctctcgtcgg aaagtgcgtg cacgacaacg agcatgtgac
     9301 gttgatgcgc gtggatcgcc gcgtagcgag cgaggcgctc gtcgagacca tcgtcatcga
     9361 gcgcgatctt gatgatgcgc gcaccttcct gctgcaaacg atcgatggcg tcggtcacgc
     9421 acgacggcga gtcgcagccg atgccgtaac catcagagcc ccacgcgtcg agcggatagc
     9481 cgttcgggtg cgtcagcatc ggacctgctt gtatgacctt gattggatac ttcgtgccga
     9541 gcgcactctc gggcgccgcg aggtctacag cagcgctcac accatgcttc gcgagctgat
     9601 ccgcgactgg gtagtacgcg aggtggacgt gcgaatcgac gatcgggagc cagtcgcgcg
     9661 agcgttcgtc gacgggcgtg tgcgtctcga caggcgcagt cggcgcaggc gatgtcggtg
     9721 agctgcacgc cgcaacgacc atcaacgcga gaccgcgcac aaccgaagta cgcatcagcg
     9781 tcgccgccgg atcagtattg gcagagcgac gcgaacatgc cgctcgtcgc gacttccgtt
     9841 gcatcgcacg gccgatcatc ccactgcttg tcgacgcgcg caccggcgat gatcacgcag
     9901 tcctcttgat acgtcgcgcc tacgccgccg ctgtttggct cgcccgcgtg ccagttcgtg
     9961 aacgtgagcg cggagccgtc ggcccagagg aacgtgcctt cggtggtgcg atcgtcggca
    10021 ccgatccacg tgttggcagt gccgacgaag ctctcggcga gcgtgtcgag cgcggcggac
    10081 ttgatgtacg cgaggtggcc accgggcgcg agcgcggcgc aggcggcctt cgcttggacg
    10141 tacgtctgcg gcgtcgtgat caggccgaag cacgagccgt ccggcgcgag catcgcgggc
    10201 gtgccaccga cacagacgcg agcgtcgggc ggcgcgtcga tgccggcatc gcggtagacc
    10261 ttcgcgtcga cgccctcgaa gttggtctga tcatcgccgc cgagcgacgc gccgcaggca
    10321 gccgacagag agatgaccgt caggccgacg aaagcacgcg agggtaccgc cggccgcatc
    10381 ttctttattg tgcccgatcg tgtaggtttc ggaacacatg agtcgtcaag ccccccttgt
    10441 cttcatcatg gcggccggcc tcggcacccg gatgaagagc gatacgtcca aggttcttca
    10501 tcacatcgcc gggcggccca tgctgcactg ggtcgtgacg gcggcgcgga cagcgggtgc
    10561 ggagcgggtt gtcgcgatct tgggtcatca gaacgaggcc gtgaaggcgt cgctcgatgg
    10621 aagcttcgga gttggcagcg tcgacgtcgc gctgcagccg gagcagcgcg ggaccgggca
    10681 cgcggtgcag tgcgcattgc ccaccgtgca gaacgagagc gaggaccgca tcgtggtcat
    10741 cctcagtggc gacgcgccgc tgttgcagac cgagcgtatt agcgagctcg tgcgtgcgtg
    10801 tgcagaagcg ccgggcggca tggcgctgct ctcgacgcga cccgatcgcg acatgccgta
    10861 tggccggctc gttcgcgatg cgaagggcgt gctcgagaag atcgtcgagc atcccgatgc
    10921 cacgccggag cagcggcaga tcaaagatac gaatgcaggc ttctacgcca ttcggctcgg
    10981 acatctgcgc cgcgacctcc aggatctgcg cgccaataat gcgaagggcg agctctatct
    11041 gacggacctc gttgccgcgg cgta
//
LOCUS       DATLBZ010000356         8389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2655871, whole genome shotgun sequence.
ACCESSION   DATLBZ010000356 DATLBZ010000000
VERSION     DATLBZ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655871"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VMZ53_18400"
     CDS             <1..451
                     /locus_tag="VMZ53_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-containing monooxygenase EthA"
                     /protein_id="HUS30490.1"
                     /translation="VLEADLIVTATGLVMRPMGGIKLVVDGNPVDPPSTMLYRGMMLS
                     GVPNYAFSIGYTNASWTLKCDLTSQYVARLLNHMRDHGYTRAMPRRDPNVSEVPAIDF
                     SSGYVQRGVGLFPRQGDRVPWRLYQNYPLDLYLIRYASIRDPALQFS"
     gene            complement(452..745)
                     /locus_tag="VMZ53_18405"
     CDS             complement(452..745)
                     /locus_tag="VMZ53_18405"
                     /inference="COORDINATES: protein motif:HMM:NF009508.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HUS30491.1"
                     /translation="MPYFALTYHTAPDYLERRAKYRAEHLALARAAHERGELLLAGAY
                     QNPPDAALLVWKCADRAPIEAFVAADPYVKNGLIARHEIRDWYVVIADDPMPL"
     gene            826..1755
                     /locus_tag="VMZ53_18410"
     CDS             826..1755
                     /locus_tag="VMZ53_18410"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30492.1"
                     /translation="MRSSLVLVLFAAIGCSGEDGDSIPESKLAFTVTSSDFTLQPGQE
                     TTKCFYFHTTNTEQVLVNKWVSDMTEGSHHMIAFSSLGTQPADGTVDDCDGADAPLPM
                     FGTQVPHEELAFPTDDGFGKPLAQLVEPNTAAFFQMHYLNSTDEPLQVHVSLSAYALP
                     ATVTEYTRTDLFATYNNDIAIPPGATNYKVSATCEIFDKKFWQMSTHSHKQSMATRII
                     DGAANGTMVLESTDWEHPKAERWEVPTFYQFQATTITWECEYNNTGDNANRTVYSGQS
                     AATNEMCMATGYYFPAVGPRGCVRDNGQCQCLL"
     gene            complement(1757..2191)
                     /locus_tag="VMZ53_18415"
     CDS             complement(1757..2191)
                     /locus_tag="VMZ53_18415"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS30493.1"
                     /translation="MQRITPFLMFNDGCEAAAKHYTTIFKNSVVHSISAMQADIEIDG
                     QRLLMYNGGPHFKFSEGMSLFVSCETQAEVDYYWDKLLEGGKPTKCGWLTDKFGVSWQ
                     IVPTVLMKLLGDKDRARANRAMQAMLKMVKLDIAELERAANG"
     gene            complement(2253..2996)
                     /locus_tag="VMZ53_18420"
     CDS             complement(2253..2996)
                     /locus_tag="VMZ53_18420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUS30494.1"
                     /translation="MARSEIEIKTDDGTCPASLFRPSGKGPWPGVLMFIDGLGYRPAM
                     FEIADRICEHGYLVLMPDLFYRAGAYTPPDPKKLFADDSVRAEWFKKVSSAVSQEMTM
                     RDTGAFLDVLTSQPEITGPLVGTTGYCMGAGFSLSAAGFYPERVAACAGFHPGKIASD
                     EPTSPHLLAPKMKARIYIAGATDDATFPDAMKQRLDDALTAAHLAHTVETYPARHGWV
                     PTDTPVHDPAQAERHFTQLFSLFDQTLKP"
     gene            complement(3015..4049)
                     /locus_tag="VMZ53_18425"
     CDS             complement(3015..4049)
                     /locus_tag="VMZ53_18425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS30495.1"
                     /translation="MRLLVLALVLTAVRVASAQPVAFELHGEVQQGQKPQLQVRAAEN
                     VTDLKLELDRDDGKHFSHKQASLAKGKAVIFSIGDGAAGKMSYKGTLSVKSPGGPWTE
                     SMKFDTVVRAPIKIGYDADHLDLDTRALQFKPSRTGVTSAEITVIGDDGKELAKSSAT
                     YDGKSDDWLMITWTQAAKTQAMMLKLRVATSDGAATNVELIPWSVAIDHEDVKFQTDS
                     SKIDDSETKKLDASLTKIQDVMKKSEKYMKMKLYVAGHTDTVGAPAKNRTLSLARAQS
                     IAAYFKKKGLTIPIAFAGFGEDVLKVKTADNTDEAANRRADYVLGPAAGAPPFKGPYL
                     KARVQWKQLR"
     gene            complement(4064..5083)
                     /locus_tag="VMZ53_18430"
     CDS             complement(4064..5083)
                     /locus_tag="VMZ53_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30496.1"
                     /translation="MRITLALPVLAICACEMEPLPGPSVPQPEPAKAQVALVPQPHRD
                     LEQLRDVPVARTVDEPVCEPTQRRYKGIPIPPSVYPKKYTPWFDKLCTDDRDKVIDFC
                     TKHPQDWQLICGGIGPLHIAYPPYVRARMYGQSEYPPHIVVIYRSVDAWERSLSAEQK
                     AYIKEQCPGGEDQPSSDLCGDNTPLVISFDGAPIQFAATSSAFDTPAATTPWLALDRN
                     GNRMIDDATELFGSASPLEDAIVRNGFTALAELDDNHDDVIDAKDAAFGRLLLWGDAD
                     RDRVGQPSELSAATSLVSISLSTTIDKRCDARDNCEGERATVVWRDSAGLHTGAVVDV
                     YLARH"
     gene            5248..6195
                     /locus_tag="VMZ53_18435"
     CDS             5248..6195
                     /locus_tag="VMZ53_18435"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HUS30497.1"
                     /translation="MRRMISAGLLALCACGNTNAAGDDQSGDDDGMQPDAATPDTPTV
                     AGRPTIFTIVLENKNDEAIYGSTNAPYLNSLIAMGALATNYKDTAHPSLPNYLHMISG
                     ANQYPGVVDVSPTQVPYFPADKPNLGTQLQAANLKWRSYQESMGTPCKLSNDGKYAPK
                     HDPFLYFTDMQKGANGLCAATNVDYSLFEADLATNEYDYMWITPNLDNDGHDPASDPA
                     LGLRNSDMWMSHEVPKILASTGFKAGGILFITWDEDEGGNNEKIPMIIVSPRVKAAGM
                     TSDTAYTHASYLATVEELLSLPRLDTVAQTPSMMEFLKP"
     gene            complement(6197..6766)
                     /locus_tag="VMZ53_18440"
     CDS             complement(6197..6766)
                     /locus_tag="VMZ53_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30498.1"
                     /translation="MRTVLLAASVLLAGCPIDRFDHIERPARFEVGLNSRHYSADPTT
                     TAARELTPPAGNHDIDGTATSASFRFTMRSRWNTYMGAEAEAGTFARPGSNLAGGYGV
                     IGASAPLHRLTLSTELAAGWRGLRDDVQDDDTNVGVLEPRVRGEMWLSPQVTFGATAG
                     ADLVGQHAWMAGFYFAVHSHSFNVLSPNR"
     gene            6804..7439
                     /locus_tag="VMZ53_18445"
     CDS             6804..7439
                     /locus_tag="VMZ53_18445"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUS30499.1"
                     /translation="MALKLHGTPLSHFTRKVRILLGELGVQYEMVWLPSVMASSPGVF
                     GDNPLMRVPTLVDGDRMLIESDHIARYIVGRYDAADWFGVKSDDPSAMNLLAVANGIM
                     SNEVVIILAQRGGLEDIDSVAYFRKLKAAMEEGLAWLDRNLKLDAPMTYVDITLISMW
                     QHLHHYKIMPLERYPRIVERVGQFLARPAVAATTPEASLAAATAAGWKPPS"
     gene            7477..>8389
                     /locus_tag="VMZ53_18450"
     CDS             7477..>8389
                     /locus_tag="VMZ53_18450"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase/hydrolase"
                     /protein_id="HUS30500.1"
                     /translation="MARLDPHSYCDDTQAQTESFSLTAKVDFATKTLAAEVTLTFKAP
                     SQGRLDLDTRDLAVEHVQDATGAALPFQLHAPEPHIGQRLAIELAAPTPSITIRYKTS
                     PDASALQWLAPAQTKGGKQPFLFSQCQAIHARSIVPLQDTPRLRVRFTAELTFPRELT
                     LVMAAADRGRHEDGSAAIHRFEMPQPIPPYLFAFAIGDLASRDLSPRCRVWAEPAVVD
                     AAAWEFADVEAMIGAAEALFGPYDWERFDILTMPPSFPYGGMENPRLTFLTPTVIAGD
                     RSLVSVVAHELAHSWTGNLVTNANAEHF"
BASE COUNT         1483 a         2706 c         2685 g         1515 t
ORIGIN      
        1 cgtgctcgag gccgacctca tcgtcacggc gacaggcctc gtcatgcggc cgatgggcgg
       61 catcaagctc gtcgtcgatg gcaatcccgt cgatccaccg tcgacgatgc tctatcgcgg
      121 catgatgctg tccggcgttc cgaactacgc gttctcgatc ggctacacga acgcgtcgtg
      181 gacgctgaag tgcgatctga cgagccaata cgtcgcgcgg ctgctcaatc acatgcgcga
      241 tcacggatac acgcgggcga tgccgcgtcg tgatccgaac gtgagcgaag tgccggcgat
      301 cgatttctcg tcgggctacg tgcagcgcgg cgtcggcctg tttccgcgcc aaggcgatcg
      361 cgtaccgtgg cggctctacc agaactaccc gctcgatctc tatctgatcc gctacgcctc
      421 gatccgcgac cccgcgctgc agttctccta gctacagcgg catcggatcg tcggcgatca
      481 cgacgtacca gtcgcggatc tcgtggcgag cgatgagccc gttcttgacg tacggatcgg
      541 cggcgacgaa cgcttcgatc ggcgcgcggt cggcgcactt ccagacgagg agcgccgcgt
      601 cgggcggatt ctggtacgcg cccgcgagca acagctcacc acgctcgtga gccgctcgcg
      661 cgagcgcgag atgctcggcg cggtacttgg cgcggcgttc gaggtagtcg ggggctgtgt
      721 gataggtcag cgcgaagtag ggcatggtcc gagggtgagc cgaatcacct ggaaccatca
      781 tggcattgct tgctacgctc cgcgccacct gagggggtag ttccaatgcg cagctcgctc
      841 gtgctcgtat tgtttgcggc gatcggatgc agtggtgaag acggcgactc gattcccgag
      901 tcgaagctgg cgttcaccgt gaccagcagc gacttcacgc tgcagcccgg ccaagagacg
      961 acgaagtgct tctacttcca cacgacgaac accgagcagg tgctcgtcaa caagtgggtc
     1021 tcggacatga ccgagggcag ccatcatatg atcgcgttct cgagcctcgg cacgcagccc
     1081 gccgacggga cggtcgatga ctgcgatggc gcagacgctc cgcttcccat gttcggcacc
     1141 caggtccctc acgaagagct cgcgttcccc acggatgatg gcttcggaaa gccgctcgcg
     1201 cagctcgtcg agccgaacac ggccgcgttc ttccagatgc actacctgaa ctcgacggac
     1261 gagccgctcc aagttcacgt cagcctctcc gcgtacgcgt tgccggcgac ggtcaccgag
     1321 tacacgcgca ccgatctgtt cgcgacgtac aacaacgaca tcgcgattcc gccgggcgcg
     1381 acgaactaca aggtcagcgc tacctgcgag atcttcgaca agaagttctg gcaaatgagc
     1441 acgcactcgc ataagcagtc gatggcgacg cgaatcatcg acggtgcggc gaacggcacg
     1501 atggtcctcg agagcacgga ctgggaacac ccgaaggccg agcgctggga agtcccgacg
     1561 ttctatcaat tccaggcgac gacgatcacc tgggagtgcg agtacaacaa caccggcgac
     1621 aacgcgaacc gcacggtcta ctccggccag tccgcggcga caaacgagat gtgtatggcg
     1681 acgggctact acttcccggc tgtcggtccg cgcggctgcg tcagggacaa cggccagtgt
     1741 cagtgtctgc tctgagctag ccgttcgctg cgcgctcgag ctcggcgatg tcgagcttga
     1801 ccatcttgag catcgcttgc atcgcccggt tcgcgcgtgc gcgatccttg tcgccgagca
     1861 gcttcatgag cacggtcggc acgatctgcc aggacacgcc gaacttgtcg gtgagccagc
     1921 cgcacttggt cggcttgccg ccttcgagca gcttgtccca gtagtagtcg acctcggcct
     1981 gcgtctcgca gctcacgaac agcgacatgc cctcgctgaa cttgaagtgc gggccgccgt
     2041 tgtacatgag caggcgctga ccatcgatct cgatatcggc ctgcatcgcg gagatcgagt
     2101 ggacgacgga attcttgaag atggtcgtgt agtgcttcgc ggcggcttcg cagccatcgt
     2161 tgaacatcag aaacggcgtg attcgttgca tagagctagc tccttgtacc tggacgacga
     2221 acgagcggcg cccagatcga cacgcgaccg tctcaaggct taagtgtttg atcgaacagg
     2281 gagaacagct gcgtgaagtg gcgctcggcc tgcgcgggat cgtggaccgg cgtatcggtc
     2341 ggcacccagc catggcgcgc cgggtacgtc tcgacggtgt gcgcgaggtg cgcggccgtt
     2401 agcgcgtcgt cgagccgctg cttcatcgcg tcggggaagg tcgcgtcgtc ggttgcaccg
     2461 gcaatgtaga tgcgcgcctt catcttcggc gcgagcaggt gcgggctcgt cggttcgtcg
     2521 ctcgcgatct tgcctgggtg gaagcccgcg caggccgcca cgcgctcggg atagaagccc
     2581 gccgcggaga gcgagaaccc ggcacccata cagtagccag tcgtgccgac gagcggaccc
     2641 gtgatttctg gctgcgatgt gagcacgtcg aggaacgcgc ccgtatcgcg catcgtcatc
     2701 tcttggctca cggcgctcga gaccttcttg aaccactcgg cgcgcacgga atcgtcggcg
     2761 aatagcttct tcggatccgg cggcgtgtac gcgcccgcgc gatagaagag gtcgggcatc
     2821 agcacgaggt agccgtgctc gcagatgcga tccgcgatct cgaacatcgc ggggcggtag
     2881 ccgagaccgt cgatgaacat gaggacgcca ggccaaggtc ccttaccgct cgggcggaag
     2941 agcgacgctg ggcacgtacc gtcgtcggtc ttgatctcga tctcgctgcg agccattggc
     3001 ggtttgtacc gctgttagcg cagctgcttc cactgcacgc gcgccttcaa ataggggccc
     3061 ttgaacggcg gcgccccggc ggctggaccg aggacatagt cggcgcgccg gtttgcggcc
     3121 tcgtcggtgt tgtcggcggt cttgaccttg aggacgtcct cgccaaagcc cgcgaacgcg
     3181 atcgggatcg tcaggccctt cttcttgaag taggcggcaa tggactgggc gcgtgcgagc
     3241 gagagcgtgc ggttcttcgc cggtgcgccg acggtatcgg tgtgacccgc gacgtagagc
     3301 ttcatcttca tgtacttctc gctcttcttc atcacgtcct ggatcttcgt gaggctcgca
     3361 tcgagcttct tcgtttcgct gtcgtcgatc ttcgaggagt ccgtctggaa cttgacgtcc
     3421 tcgtgatcga tcgccaccga ccacggaatc agctcgacgt tcgtcgcggc gccatcagac
     3481 gtcgccacgc ggagcttcaa catcatcgcc tgcgtctttg cggcctgcgt ccacgtgatc
     3541 atcagccagt cgtcggactt gccgtcgtac gtcgcggagc tctttgcgag ctccttgccg
     3601 tcatcgccga tcaccgtgat ctcggcgctc gtgacaccgg tgcgcgacgg cttgaactgc
     3661 agtgcgcgcg tatcgagatc gagatggtcg gcgtcgtagc cgatcttgat cggcgcgcga
     3721 accaccgtgt cgaacttcat gctctcggtc cacggcccgc cgggcgactt caccgagagc
     3781 gtgcccttgt agctcatctt gccggcggcg ccgtcgccga tcgagaagat gacggccttg
     3841 cccttcgcga ggctcgcttg cttgtggctg aagtgcttgc cgtcgtcgcg gtcgagctcc
     3901 agcttcaggt ccgtcacgtt ctcggcggcg cgcacttgca gctgcggctt ctggccttgc
     3961 tgcacctcgc cgtggagctc gaatgcaaca ggctgcgcgc tcgcgacgcg tacagcagtg
     4021 aggaccaacg ccaagacgag gagtcgcacg tcctcgagta tagctagtga cgggcgagat
     4081 acacgtcgac gaccgcgcca gtgtgtaggc ccgcggagtc gcgccagacc accgtcgcac
     4141 gctcgccctc gcaattgtcg cgagcgtcac agcgcttgtc gatcgtcgtc gacagcgaga
     4201 tcgaaaccag cgatgtcgcg gctgacagct ccgatggttg accgacgcgg tcgcgatcgg
     4261 cgtcgcccca caggagcaga cgaccaaacg ccgcatcctt cgcatcgatc acatcgtcgt
     4321 ggttgtcgtc gagctctgcg agtgccgtga agccgttgcg aacgatcgca tcctcgagcg
     4381 gcgaggcgct gccgaatagc tcggtggcat cgtcgatcat tcggttgccg ttgcgatcga
     4441 gcgctagcca tggcgtcgtc gcagccggtg tatcgaacgc ggagctcgtc gccgcaaatt
     4501 gaattggtgc gccgtcgaac gagatgacga gcggcgtgtt gtcgccacac agatccgacg
     4561 acggctggtc ctcaccaccg gggcactgct ccttgatgta cgccttctgc tcggcggaca
     4621 gcgatcgctc ccacgcgtcg accgatcgat agatcacgac gatgtgtggc ggatactcgc
     4681 tctggccgta catgcgcgcc cgcacgtagg gcggataggc aatatggagc gggccgatgc
     4741 cgccacagat cagctgccag tcctgcggat gcttcgtgca gaagtcgatc accttgtcgc
     4801 gatcgtcggt gcagagcttg tcgaaccacg gcgtgtactt cttgggatag acgctcggcg
     4861 ggatcgggat gcctttgtag cgccgctgcg tcggctcgca gaccggttcg tcaacggtac
     4921 gagcgaccgg gacgtctcga agttgctcga gatcgcggtg cggctgcggc accagcgcga
     4981 cctgcgcctt tgcgggctcg ggctgcggca ccgacgggcc gggcagcggc tccatctcgc
     5041 atgcgcagat tgccaggacg ggaagggcca gcgtgattcg catccgtatg cgaacgcatg
     5101 aaatgcttaa tggttttgcg acgcttttgg gcgcaaccga cgccggtcac gcgtgtcggc
     5161 ttcagttcgt gacaccgcgg ttaccatttt cgggcgcccg cgtttttctc gccaacgcgc
     5221 gaaacgcctg cggtacgact ccgcgcgatg aggcggatga tctcggcagg tctgctcgcg
     5281 ctctgcgcgt gcggaaacac caacgcagcg ggtgacgatc aaagcggcga cgacgacggc
     5341 atgcagcccg atgccgcgac accggacacg ccgaccgtcg ctggtcgtcc gacgatcttc
     5401 acgatcgtgc tcgagaacaa gaacgacgaa gcgatctacg gctcgacgaa tgcgccctat
     5461 ctcaactcgc tgatcgcgat gggcgcgctc gcgacgaact acaaagacac cgcgcatcct
     5521 tcgctgccga actacctgca catgatcagc ggtgcgaatc agtacccggg cgtcgtcgac
     5581 gtgagcccga cgcaggttcc gtacttcccc gcggacaagc cgaacctcgg cacgcagctg
     5641 caggctgcca atctcaagtg gcgctcgtat caagagagca tgggcacgcc gtgcaagctg
     5701 tcgaacgacg gcaagtacgc gccgaagcac gacccgttcc tctacttcac cgacatgcag
     5761 aagggcgcga acggcctctg cgcggcgacg aacgtcgact actcgctgtt cgaagcggac
     5821 ctcgcgacga acgagtacga ctacatgtgg atcacgccga acctcgacaa cgacggccac
     5881 gatcccgcca gcgatcccgc gctcggactg cgcaactcgg acatgtggat gtcgcacgaa
     5941 gtcccgaaga tcctcgcgag caccggcttc aaagcgggtg gcatcctgtt catcacgtgg
     6001 gatgaggacg aaggcggcaa caacgagaag attccgatga tcatcgtgtc accgcgcgtg
     6061 aaggcggcag gcatgacgtc ggacaccgcg tatacgcacg cgagctatct cgcgaccgtc
     6121 gaagagctgc tctcactccc tcggctcgat accgtcgcgc agacgccgag catgatggag
     6181 ttcttgaagc cctgattcag cgattcggcg acagcacgtt gaacgagtgc gagtggacgg
     6241 cgaagtagaa gcccgccatc cacgcgtgct ggcccacgag atccgcgccc gcggtcgcgc
     6301 cgaacgtcac ctgcggtgac agccacatct cgccgcgcac gcggggctcg aggacgccga
     6361 cgttcgtgtc gtcgtcctgg acatcgtcgc gcaggccgcg ccagccagcc gcgagctctg
     6421 tcgagagcgt gaggcgatga agcggagcgc tcgcgccgat cacgccgtag ccacctgcga
     6481 ggttcgagcc cgggcgcgcg aatgttccag cttccgcctc ggcgcccata taggtgttcc
     6541 agcggcttcg catcgtgaag cgaaacgacg cgctcgttgc cgtgccgtcg atgtcgtgat
     6601 tgcccgcagg cggagtgagc tcgcgcgctg ccgttgttgt tggatcggcc gagtagtggc
     6661 gcgagttgag cccgacttcg aagcgcgcgg gacgctcgat gtggtcgaag cgatcgatcg
     6721 gacaacccgc gagcagtaca gatgcagcga ggaggacggt gcgcacggct ccgatgctag
     6781 caccgggtgc tatcgtcgtc gcgatggcgc tgaagctcca cggcacgccg ctctcgcact
     6841 tcactcgcaa ggtgcggatc ctcttgggag agctcggcgt ccagtacgag atggtctggc
     6901 ttccgagcgt catggcgtcg agcccgggag tgttcgggga caacccgctg atgcgcgtgc
     6961 cgacactcgt cgacggcgac cgcatgctga tcgaatcgga tcacatcgcg cgctacatcg
     7021 tcggtcgata cgacgcggcc gattggttcg gcgtcaagtc cgacgatccg tcggcgatga
     7081 acctgctcgc agtcgcgaac gggatcatga gcaacgaggt ggtcatcatc ctggcgcaac
     7141 gcggcgggct cgaggacatc gatagcgtcg cgtacttccg caagctcaaa gcggcgatgg
     7201 aagagggcct cgcgtggctc gatcgcaacc tgaagctcga cgcgccgatg acgtacgtcg
     7261 acatcacgct catctcgatg tggcagcacc tgcaccacta caagatcatg ccgctcgagc
     7321 gctatccgcg gatcgtcgag cgagtggggc agttcctcgc acgtcctgcg gttgcggcga
     7381 ccacaccaga ggcgtcactc gctgcggcca ccgccgccgg atggaagccg cctagctgag
     7441 atcccatcca gcgcgcggca tgtcacagtc ccggcgatgg cgcgcctcga tcctcattcg
     7501 tattgcgatg acacgcaggc ccaaaccgaa tcgttttcgc tgaccgcgaa ggtcgacttc
     7561 gcgacgaaga cgctcgccgc agaagtgacg ttgacgttca aggcaccgtc gcagggtcgc
     7621 ctggatctcg atacgcgcga tctcgcggtc gagcacgtac aggatgcgac cggtgcggcg
     7681 ctgccatttc agctccacgc gccggagccg cacatcggcc aacggctggc gatcgagctc
     7741 gctgcgccca cgccatcgat cacgattcgc tacaagacgt cgcccgacgc atcggcactg
     7801 cagtggctcg cgcccgcgca gaccaagggc ggcaagcagc cgtttctgtt ctcgcagtgt
     7861 caggcgattc acgcgcgctc gatcgtgccg ctgcaagaca cgccgcgcct gcgcgttcgc
     7921 ttcaccgcgg agctgacgtt cccgcgcgag ctcacgctcg tgatggccgc ggcggatcgc
     7981 gggcggcacg aggatggcag cgcagcgatc catcgcttcg agatgccgca gccgattccg
     8041 ccgtacctgt tcgcgttcgc gatcggcgac ctcgcgtctc gcgatctctc accgcgctgt
     8101 cgcgtatggg cggagcccgc ggtggtcgac gcagcagcgt gggagttcgc cgacgtcgaa
     8161 gcgatgatcg gcgcggccga ggcgctgttc ggtccctacg attgggagcg cttcgacatc
     8221 ctgacaatgc ctccttcgtt tccgtatggc ggcatggaga acccgcgcct gacgttcctc
     8281 acaccgacgg taatcgcggg cgaccgctcg ctcgtctctg tcgtcgcgca cgagctcgct
     8341 cactcgtgga cgggcaacct cgtcacgaac gcaaacgccg agcacttct
//
LOCUS       DATLBZ010000357         4645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1911548, whole genome shotgun sequence.
ACCESSION   DATLBZ010000357 DATLBZ010000000
VERSION     DATLBZ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911548"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..472)
                     /locus_tag="VMZ53_18455"
     CDS             complement(95..472)
                     /locus_tag="VMZ53_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30501.1"
                     /translation="MTRRPKFAAAAAVALFVVGQLAALVHEAETRHVTCAEHGEQLEA
                     ATLDGHDDLCGQSHLIAVDGNTGAHEDCDIARLLRTSTRTSQAPVLHVATDALATHEA
                     VIAIDGPRAADVLVNAPKTSPPA"
     gene            complement(535..1041)
                     /locus_tag="VMZ53_18460"
     CDS             complement(535..1041)
                     /locus_tag="VMZ53_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30502.1"
                     /translation="MERLRRALRGADRGEKPALPVGEAMHPVALLAVVLLIVNDWVLK
                     PALHDGSALGELVTGKLSDIAGLAFAPVVLTAAIGLVLAIAAKLGARVTPYLTQRRLV
                     LAIAATGITFAAVKLSPAAAEALANALSHLGRRASIYLDRWDLLTLPALAVAYWIGRD
                     ELARLRPA"
     gene            1060..2775
                     /locus_tag="VMZ53_18465"
     CDS             1060..2775
                     /locus_tag="VMZ53_18465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS30503.1"
                     /translation="MSESGKQSESAKQLAEAEAKAKERFEKAMTELREGAYRFSSRAR
                     GQSELIIEELIDPDGDTVASLAEVEGEAGALALAQSLELITFDTWLFGQIGDKDALDL
                     KAEEHWEVWFTFGAWIGETMRRRHGGHWLLMGDDPHQWRLGFSKIFLEIAPFMFAEQL
                     LRMGSGATRKMVTEVERIRQLHEPEPSITGQLPFEAGKAELLPQVQQSLTEIARFLNE
                     SQHVELVEIEGHVDESDAPNDDARQTLSKKRADTVVAFLVERGVNAKRLEAKGYGADH
                     PSREAQGETPNPDANRRVQFDVLEAKPVVLLDRFLAQHYIRMHTVPLGQWMSMDFAGL
                     AKLWNEEPCEKLVAVLKERGPRLGPQNFQVVEQVVAALSRAKQDVPVAKQTGDRGLFE
                     AIAQIIALRHATAPVAIDILERIVMPALHVGMPDKFPPLDEDDLSNLRKGIELFAFFT
                     EVMPHKYQADDEGFLGTIPHDQLTTPYADRNVLEIGKGDWVVVNPAHFIPMLGEFDPQ
                     KFLEKYDEFVKYVRATPDAPTRRDDGRMLAETAIRALADLKMCVAVCAQNKDALLFRL
                     LPPPG"
     gene            complement(2850..4046)
                     /locus_tag="VMZ53_18470"
     CDS             complement(2850..4046)
                     /locus_tag="VMZ53_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30504.1"
                     /translation="MVWLAPCLAAVVLGGCGKKKENDKPAEKVVAKEGDKKPAEPPKK
                     VGLEDPTSDPKIVAAAKQVIADCGKTMEEKSGEKTKMLDACDKFGATFGDQKFEKAEV
                     TMVNFLEDPDVRVRFMGLVGLHRFAYAYKEDKALVGRVLDALKKEKAPSPIDHLWVQR
                     VSRLPADDADVDAKLKAIGMDAAASNDVKVSIMSWWPGAAGYELVKANAASTDAAMVK
                     AVAQGYGQQFANHADEACAYWAAHLETPDADTNDVTLGHLTGGYLSISTKDTESTDSI
                     SGDTEGPSSRNEKRCTAEQQGKALDVIEKKFAAGDSGDVMPRSLGFLANDKLTSKEIK
                     ERAVKDLKAMVEKKGYFPGREALRQLNEAGDPAMVKYAQKFSKDADLKETVDEIVKAS
                     KEKKNK"
     gene            complement(4082..>4645)
                     /locus_tag="VMZ53_18475"
     CDS             complement(4082..>4645)
                     /locus_tag="VMZ53_18475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS30505.1"
                     /translation="KQLADCREIVEEEIAGLRRPVDTFRTLGQLPKVEKAPIALEEVA
                     EELRLDPIYAAKLELRPSAESVTVRADKLLLKRVLANLVENGIHAGQDAKTEGKVVIS
                     WWTQRDVVAIAVDDQGKGVAEADRDKIFEPYVTTKSTGTGLGLAIARKIAIEHGGELS
                     LATEKAPTGGARFVVTLPLRGPEASMA"
BASE COUNT          772 a         1580 c         1487 g          806 t
ORIGIN      
        1 ggacggcgag ctcatcgttc gaagcacgag cgcggcgaac ggcgagctcg tcattcggtc
       61 gcggtcagcg cggcgaacgg cgagcgcctt gcgctcaggc cggcggcgaa gttttcggcg
      121 cgttgacgag gacgtcggcg gcgcgaggcc catcgatcgc gatgaccgct tcgtgcgtcg
      181 cgagggcgtc cgtggcgacg tgcagcacgg gcgcctgcga agtgcgcgtg ctcgtgcgga
      241 gcagccgcgc gatatcgcaa tcctcgtgcg cgccggtgtt gccatcgacg gcgatgaggt
      301 gcgattggcc gcacagatcg tcgtggccgt cgagcgtcgc ggcttcgagc tgttcgccgt
      361 gctcggcgca cgtgacgtgt cgcgtctccg cttcgtgcac gagcgccgcg agctggccga
      421 cgacgaatag cgcgacagca gctgctgcag cgaatttcgg ccggcgcgtc acgcgcagaa
      481 catcgcatag cggcgactgg tttgcagctg cgcaaaacgc ccggtcgcgc ggcctcacgc
      541 cggtcgcaag cgcgcgagct cgtcgcggcc aatccagtag gcgacggcga gtgcgggcag
      601 cgtgagcaaa tcccatcgat cgagatagat cgatgcgcgg cggccgaggt gggacagcgc
      661 gttcgcgagc gcttcggccg cagcggggct cagctttacc gcggcgaacg tgatgccggt
      721 cgcggcgatt gcgaggacga ggcgacgctg cgtgaggtac ggggtcacgc gcgcgccgag
      781 cttggccgcg attgcgagca cgaggccgat ggcggcggtg agcacgaccg gcgcgaacgc
      841 gaggcccgcg atgtccgaga gcttgccggt aaccagctcg ccgagcgcgc tgccgtcgtg
      901 cagggcaggt ttcagcaccc aatcgttgac gatcagcaaa acgacggcga gcagcgcgac
      961 ggggtgcatg gcctcgccga cgggaagtgc gggcttttcc cctcgatcgg cgccgcgcag
     1021 cgctcgccgc aaccgctcca ttcggatact ctgcgcctcg tgtccgagtc cggcaagcag
     1081 tccgagagtg ccaagcaact cgccgaagcc gaagcgaaag ccaaggagcg gttcgagaag
     1141 gccatgaccg agctccgcga gggcgcctat cgcttctcct cgcgcgcgcg cggccaatcc
     1201 gagctcatca tcgaagagct catcgatccc gatggcgata cggtcgcgtc gctcgcggaa
     1261 gtcgaaggcg aggccggcgc actcgcgctc gcgcagtcgc tcgagctgat cacgttcgac
     1321 acgtggctgt tcggtcagat cggcgacaaa gacgcgctcg atctcaaagc cgaagagcac
     1381 tgggaagtgt ggttcacgtt cggcgcgtgg atcggcgaga cgatgcgacg ccgccatggc
     1441 ggccactggc tgctgatggg cgatgacccg catcaatggc gcctcggctt ctcgaagatc
     1501 ttcctcgaga tcgcgccgtt catgttcgcg gagcagctcc tgcgcatggg ctcgggcgcg
     1561 acgcgcaaga tggtcacgga agtcgagcgc atccgccagc tgcacgagcc agagccgtcg
     1621 atcacgggcc agctcccatt cgaagccggc aaggccgagc tcctgccgca ggtccagcag
     1681 tcgctcaccg agatcgcgcg cttcctcaac gagagccagc acgtcgagct cgtcgagatc
     1741 gaaggccacg tcgacgagag cgacgctccc aacgacgacg cacgccagac gctgtcgaag
     1801 aagcgcgccg atacggtcgt cgcattcctc gtcgagcgcg gcgtcaacgc gaagcgcctc
     1861 gaagcgaaag gctacggcgc cgatcacccg agccgcgagg cgcaaggcga gacgccgaac
     1921 cccgacgcaa accgtcgcgt gcagttcgat gtcctcgaag ccaagcccgt cgtgctcctc
     1981 gaccgcttcc tcgcgcagca ctacatccgc atgcacacgg tcccgctcgg ccagtggatg
     2041 agcatggact tcgcgggcct cgcgaagctc tggaacgaag agccgtgcga gaagctcgtc
     2101 gccgtcctca aagagcgcgg cccgcgcctc ggcccgcaga acttccaggt cgtcgagcag
     2161 gtcgtcgccg cactgtcacg cgcgaagcaa gacgtacccg tcgcgaaaca aaccggcgac
     2221 cgcggtctgt tcgaggcgat cgcccaaatc atcgcgcttc gtcacgcgac cgcgcccgtc
     2281 gcgatcgaca tcctcgagcg catcgtgatg ccggcgctcc acgtcggcat gccggacaag
     2341 tttccgccgc tcgacgaaga cgacctctcg aacctgcgca aaggcatcga gctgttcgcg
     2401 ttcttcaccg aagtgatgcc gcacaagtac caagccgacg acgaaggctt cctcggcacg
     2461 atcccgcacg accagctcac gacgccgtac gccgatcgca acgtgctcga gatcggcaag
     2521 ggcgactggg tcgtcgtgaa ccccgcgcac ttcattccga tgctcggcga gttcgatccg
     2581 cagaagttcc tcgagaagta cgacgagttc gtcaaatacg tgcgcgcgac ccccgacgca
     2641 ccgacgcgcc gcgacgacgg ccggatgctc gcggagactg ccatccgcgc gctcgccgat
     2701 ctcaagatgt gcgtggcggt ctgcgcgcag aacaaagacg cgctgctctt ccgcctgctg
     2761 ccgccgccgg gctagcaacg cgcgccgcgc cgcagggcta acgacgcgcc gcgccgcagg
     2821 cctaacgacg cgcgcgagcg cgccgcgagc tacttgttct tcttctcttt cgaagccttc
     2881 acgatctcgt cgacggtctc tttgagatcg gcgtccttcg agaacttctg cgcgtacttc
     2941 accatcgcgg gatcgccggc ctcgttgagc tggcgaagcg cctcgcggcc ggggaagtag
     3001 cccttcttct cgaccatcgc cttgaggtcc ttcacggcgc gctccttgat ctccttcgac
     3061 gtgagcttgt cgttcgcaag gaacccgagc gagcgcggca tcacatcgcc gctatcgccg
     3121 gcggcgaact tcttctcgat cacgtcgagc gctttgccct gctgctcggc cgtgcagcgc
     3181 ttctcgttcc tcgagctcgg cccctcggtg tcgccgctga tggagtccgt gctctcggtg
     3241 tccttcgtcg agatgctgag atagccaccg gtgaggtgac cgagcgtgac gtcattggta
     3301 tccgcatctg gcgtctcgag gtgagcggcc cagtacgcgc acgcttcgtc ggcgtggttc
     3361 gcgaactgct ggccgtagcc ctgcgcgacc gccttgacca tcgccgcgtc ggtgctcgcc
     3421 gcattcgcct tcacgagctc gtagccggcg gcgcctggcc accacgacat gatcgacacc
     3481 ttgacgtcgt tcgacgccgc ggcatccatc ccgatcgcct tgagcttcgc gtcgacatcc
     3541 gcgtcgtccg cggggagccg cgaaacgcgc tgaacccaca gatggtcgat cggcgacggc
     3601 gccttttcct tcttcagtgc gtcgagaacg cggccgacga gcgccttgtc ctccttgtac
     3661 gcgtacgcaa accggtgaag cccgacgagg cccataaaac ggacgcgaac gtccggatcc
     3721 tcgaggaagt tgaccatcgt gacttcggcc ttctcgaact tctgatcgcc gaacgtcgcg
     3781 ccgaacttgt cgcaggcgtc gagcatcttc gtcttctcgc cgctcttctc ctccatcgtc
     3841 ttgccgcaat cggcaatcac ttgcttcgcc gcggcgacga tcttgggatc gctcgtcgga
     3901 tcctcgaggc caaccttctt cggcggctcg gcgggcttct tgtcgccctc tttagctacg
     3961 accttctcgg ccggcttgtc attttctttc ttcttaccgc acccgcccaa aacaacagca
     4021 gcgagacacg gcgccaacca caccatcgat ttcatcattg gcgcgtaccc tatcgtagtt
     4081 ctcaggccat cgaggcttcg ggaccacgaa gaggcagcgt aacgacgaat ctcgcaccgc
     4141 ccgtcggcgc cttctcggtc gcaagcgaca gctcgccacc atgctcgatc gcgatcttgc
     4201 gcgcgatcgc gaggccgagg cccgtgcccg tcgacttcgt cgtcacgtac ggctcgaaga
     4261 tcttgtcgcg gtcggcttcg gcgacgcctt tgccttgatc gtcgacggcg atggcgacga
     4321 cgtcgcgctg cgtccaccag ctgatgacga ccttgccctc ggtcttcgca tcttggccgg
     4381 cgtggatgcc gttctcgacg aggtttgcta ggacgcgctt gagcagcagc ttgtcggcgc
     4441 gcacggtgac ggactcggcg gacgggcgca gctcgagctt cgcggcgtag atcggatcta
     4501 ggcgcagctc ttcggcgact tcttcgagcg cgatcggggc tttttcgact ttgggcagct
     4561 ggccgagcgt ccggaatgta tcgacggggc ggcgcaggcc cgcgatttct tcctcgacga
     4621 tttcgcggca gtcagcgagc tgctt
//
LOCUS       DATLBZ010000358         5631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1168199, whole genome shotgun sequence.
ACCESSION   DATLBZ010000358 DATLBZ010000000
VERSION     DATLBZ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168199"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VMZ53_18480"
     CDS             complement(<1..1226)
                     /locus_tag="VMZ53_18480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS30506.1"
                     /translation="MHGSYWHTDASWRDRWTDTPVPKHVDFAIIGAGFAGLSTAIRLR
                     EQNPSAQIIVLEAERVGFGASGRNAGFLSPLAAPVWLLGAQRSVEQAWGAARINAEVH
                     AIARSLEQYDCELAPATLALQAQSRLADAALREFAKAVELVGLEHRVVESRARPGRLY
                     LEMSAYTLHPYKLALGLAEHAERHGITIRERTRVQSVEALRAGGARVKLDDASSFEAA
                     KVVVCTNAYTPSIDVGERVAALVVHSFMAATEPAEVARDGDFTVEINATQGYHRMHRG
                     RVIYGGIDKLRMPDGDDFAVPLRERQKLESALHESFPHAALTTEHAWSGRFHATATGL
                     PIIRMATENHAIVFNVGYGGTGVALSLVCARLAAAVASHGRFTNVDDTRLLALIHGTR
                     ISVRDSVRAIARIARGA"
     gene            1250..2359
                     /locus_tag="VMZ53_18485"
     CDS             1250..2359
                     /locus_tag="VMZ53_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30507.1"
                     /translation="MGADSELHDAVTALCRDVNGIDWDAASVQADVHRMLAAVKRASQ
                     AALDAALATIVERLRISQLSDADGVAQVAISGGTLVEYGAAPRPLAEVMLSKLPAVLD
                     AARHYADTCLADLPALDEDDEEEEPEGIAHVDERAIPRDVFRSHLETDRGGGCALVGL
                     RNWALPAIAALTRDREMLRRACADAELRARANALRDSDAHWLPVLLGAELDTRWLALV
                     PGEARGFHIRVDGIVSNFDLHTLVAAALIERGVAGKAPPKSLVAFLEGRAKDAAVSHI
                     EGVWNYYDWRAAGYDLTRSVPTERWVWNEGVPSDVPKFQDMRTLLIGPPPLQRTWNVG
                     RTFSALAARVSVEEELPAERVRELLTAMKQEAKRG"
     gene            complement(2369..3382)
                     /locus_tag="VMZ53_18490"
     CDS             complement(2369..3382)
                     /locus_tag="VMZ53_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30508.1"
                     /translation="MTKPMTRLILVALVLSPALAAADDAAAEYKRGQTALKAGRVHEA
                     CDAFAASAKLDAKIETELSLADCLEQDGKIMAAARLYKKLGEKDTDTARAKKSSAKAT
                     KLEAKAPRLRFAINPKPQGLVITVDGEAIAGTDEARVDIGPHEVVGTAPGFEGHASPA
                     VDGSRPIVDVILRMEEKAEPAPPVQTREPAPAATPKPNTAPEPAPPTAAQPEAAAPMV
                     EGSITTTSHRKRNGVVLTAAGAGLAIGAGVMLGLAASKFSTERELCPNAQCANEMDRA
                     RANALLDNGTTLRTIGIGVGVTGVALLAVGGYMLMTPDHESTRVSLSVGNGSTAVVFG
                     GSF"
     gene            complement(3392..4780)
                     /locus_tag="VMZ53_18495"
     CDS             complement(3392..4780)
                     /locus_tag="VMZ53_18495"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30509.1"
                     /translation="MKLAATLACVLMACGGGGKPTAQTTTTTKTADAKPAAASVSKKP
                     DLYDRLGGQRAIVAAVDGFIGRVAADKRINIRFSNTDIPRLKSLLVDFVCMATGGPCH
                     YVGQDMETSHAGMEVVEDEFNALVEDLSLALDELKVPAKEKGELLGALGPLAPRIITP
                     ADRLKPISDAKLAKAKALLPKLTDPTAKELMEAALVAGKRGQRNYADQLFTRVEIIVG
                     ADTVAAAAPTFREGAPKRIDTAITQMDKDAPPQPKIVGSSDEDSPSTAQLGASLKGTI
                     TVDGKPLDGVGLVMLYPTNGKFAKRTPKKRVMEQRNKTFSPHLLAVPPGSTVAFPNFD
                     AFYHNVFSLSSTQPFDIGQYKNGQSRDMKFDKTGMVRLGCNVHAKMAAFLFVVDAPNY
                     VPVEGTKEFNFKSLAPGKYKARVWSERSLEPTESEISIHDGVNTMTFDVKGDAPKGPS
                     ADKFGNSRTAAN"
     gene            complement(4794..5594)
                     /locus_tag="VMZ53_18500"
     CDS             complement(4794..5594)
                     /locus_tag="VMZ53_18500"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HUS30510.1"
                     /translation="MKKLALVLFLVAGCKDENPIVPDGPPMQEVDAAVVQPPTYVQVE
                     HLARPGINEALLLTNDFNAGYNATAPSFAGAPPAAVTAITGEAKTVLKALYLGVCLLD
                     GALNLTPSTGLKPAGIQCHAVGAAIWTENTLAGVTLTPESKTAAQAYADKVFSQFIPD
                     VMRIDTSAPSGYLTLCGDANSVPLLCGGRLLNEDVIDITYNYLLAGAAVPAKGSYNQV
                     TALVSDGVNFSSDDAKNSGNAIASTGISNANQYHPDVTSTFPYSAAPF"
BASE COUNT          861 a         1819 c         1910 g         1041 t
ORIGIN      
        1 gcaccgcgcg cgatccgcgc aatggctcgc acagagtctc gcacggagat gcgcgtcccg
       61 tggatgagcg cgaggaggcg cgtatcgtcg acattcgtga agcgaccgtg cgaggcgacc
      121 gcagccgcga ggcgtgcaca cacgagtgac agcgcgacgc ccgttccgcc gtatccgacg
      181 ttgaagacga tcgcgtgatt ctccgtagcc atgcgaatga tcggcagccc ggtcgcggtt
      241 gcgtgaaagc gtcccgacca cgcgtgctcg gtcgtgagcg cggcgtgcgg aaagctctcg
      301 tgcagcgcac tctcgagctt ctgacgctcg cgcagcggga ccgcgaagtc atcgccgtcg
      361 ggcatgcgca gcttgtcgat gcctccatag atgacgcggc cgcgatgcat ccggtggtag
      421 ccctgcgtag cgttgatctc gacggtgaaa tcgccatcgc gcgccacctc ggcgggctct
      481 gtagcggcca tgaaactgtg tacgacgagc gcagccaccc gctcgccgac gtcgatcgat
      541 ggcgtgtacg cgttcgtgca gacgacgacc ttcgcggctt cgaacgacga ggcgtcgtcg
      601 agcttcaccc gtgcgccacc cgcacgtagc gcttcgacgc tctgcacgcg tgttcgttcg
      661 cggatcgtga tcccgtgccg ctcggcgtgc tccgcgagac cgagagcgag cttgtatgga
      721 tgcagcgtgt acgcgctcat ctcgagatag agccgcccgg gtctcgcgcg actctcgacg
      781 acacgatgct cgaggccgac gagctcgacc gcctttgcga attcacgcag cgctgcgtcc
      841 gcgagtcgac tttgcgcctg cagcgcgagt gtcgcgggcg cgagctcaca gtcgtattgc
      901 tcgagcgacc gcgcgatcgc atgcacctcg gcgttgatac gtgcggcacc ccacgcttgc
      961 tcgaccgagc gctgcgctcc gagcagccac accggtgcag caagtggtga cagaaacccc
     1021 gcgttgcgcc cgctcgcgcc aaacccgaca cgctctgctt cgaggacgat gatctgcgcg
     1081 ctcgggttct gctcgcgcag tcgaatcgcc gtcgagagac cggcaaaccc cgcgccgatg
     1141 atcgcgaagt cgacgtgctt cgggaccggt gtgtcggtcc agcggtcgcg ccagctcgcg
     1201 tccgtgtgcc agtagctccc gtgcatgtcg tagcggtatc gtagccgcgg tgggtgccga
     1261 ctcggaactg cacgacgctg tgaccgcgct ttgtcgcgac gtcaacggca tcgattggga
     1321 cgctgcgtcg gtgcaggcgg acgtccaccg gatgttggcc gcggtgaagc gcgcttcgca
     1381 ggccgccctc gatgcggctc tcgcgacgat cgtcgagcga ctccgtatct cgcagctgag
     1441 cgatgccgat ggggtagcgc aggtcgccat cagtggcgga acgctcgtgg agtacggcgc
     1501 ggcgccgcga ccgctcgccg aggttatgct ctcgaagctg ccggctgtcc tcgacgcggc
     1561 tcggcactac gccgatacct gcctcgcaga cctgccagcc ctcgacgagg atgatgagga
     1621 ggaagagcct gagggcatcg cgcatgtcga cgagcgcgcg atccctcgcg acgtctttcg
     1681 cagccacctc gagacggatc ggggcggcgg ctgcgcactg gtcgggctgc gtaactgggc
     1741 gttgcccgcc atcgcggcac tgacgcggga ccgcgagatg ctgcgtcgag cgtgcgccga
     1801 tgcggagcta cgagctcgcg ccaatgcgct gcgcgattcc gacgcgcatt ggttgcccgt
     1861 gctgctcggc gcggagctcg acacgcggtg gctcgctctc gtcccgggcg aagcgcgcgg
     1921 ctttcatatc cgcgtcgacg gcatcgtcag caacttcgat ctgcacacgc tcgtggcggc
     1981 cgcactgatc gagcgcggcg tcgcgggcaa ggcgccgccg aagagcctcg tcgcattcct
     2041 cgagggacgc gcgaaggacg ctgcggtctc gcatatcgaa ggcgtctgga attattacga
     2101 ctggcgcgct gccggatacg acctgacgcg aagcgttccg acggagcgtt gggtgtggaa
     2161 cgaaggcgtt ccgagcgacg tgccgaagtt tcaggacatg cgcacgctgc tgatcggccc
     2221 gccaccgctg cagcgcacgt ggaacgtcgg gcgtacgttc agcgcgctcg ccgctcgcgt
     2281 cagcgtcgaa gaagagctcc ccgccgaacg cgtgcgcgag ctgctcaccg cgatgaagca
     2341 agaagcgaag cgcggctgaa cgcggagctc agaacgagcc gccgaacacg accgcggtcg
     2401 agccgttgcc gacgctcagc gacacgcgcg tgctctcgtg atcgggcgtc atcagcatgt
     2461 agccgccgac tgcgagcagc gcgacacccg tcactccgac gccgataccg atggtgcgca
     2521 gcgtcgtgcc gttgtcgagg agcgcgttcg cccgggcgcg gtccatctcg ttcgcgcatt
     2581 gcgcgttcgg acagagctcc ctctccgtgc tgaacttcga tgcggcgagc ccgagcatca
     2641 cgcctgcgcc gatcgcgagg cccgcgcctg ccgcggtgag gacaacgccg ttgcgcttgc
     2701 gatggctcgt tgtcgtgatc gagccttcga ccatcggcgc tgccgcttcg ggctgggcag
     2761 ccgtcggcgg agccggctca ggcgcggtgt tcggcttcgg cgttgccgcg ggagccggtt
     2821 cgcgcgtctg caccggtggc gctggctccg ccttctcctc catgcgcagg atcacgtcga
     2881 cgatcggccg cgagccatcg acggcaggcg acgcgtggcc ttcgaagcca ggtgccgtac
     2941 cgacgacctc gtgaggtccg atgtcgacac gagcttcgtc ggtgccggcg atcgcttcgc
     3001 cgtcgaccgt gatgacgagg ccctgcggct tcggattgat cgcgaaccgc agccgcggcg
     3061 ctttcgcttc gagcttcgtc gctttcgcgc tcgacttctt ggcgcgggcc gtgtcggtgt
     3121 ccttctcgcc gagcttcttg tagagccgcg ccgcggccat gatcttgccg tcttgctcga
     3181 ggcaatcggc gaggctcagc tcggtctcga tcttcgcgtc cagcttggcg ctcgccgcga
     3241 atgcgtcgca cgcctcgtga acacgaccgg ctttgagtgc ggtctgccca cgtttgtatt
     3301 cggctgcagc gtcgtcggct gcggcgagcg caggcgagag gacgagagcc acgagaatca
     3361 gtcgggtcat tggctttgtc atgacgtcct cctagttggc agccgtgcgc gagttgccga
     3421 acttgtccgc gctcggtccc ttcggtgcgt cacccttgac gtcgaacgtc atggtgttca
     3481 cgccgtcgtg aatcgagatc tcggactcgg tcggttcgag gctgcgttcg ctccacacgc
     3541 gggccttgta cttgcccggt gcgaggctct tgaagttgaa ctctttcgtg ccttcgacag
     3601 ggacgtagtt cggcgcatcg acgacgaaga ggaatgccgc catcttcgcg tggacgttgc
     3661 agccgagacg caccatgccg gtcttgtcga acttcatgtc gcgcgactgg ccgttcttgt
     3721 actggccgat gtcgaacggc tgcgtcgacg agagcgaaaa cacgttgtgg tagaacgcgt
     3781 cgaagttcgg gaacgcgacc gtcgagcccg gtggcaccgc gagcaggtgc ggcgagaacg
     3841 tcttgttccg ctgctccatc acgcgcttct tcggcgtgcg cttcgcaaac ttcccgttcg
     3901 tcggatacag catcacgaga ccgacaccgt cgagcggctt tccgtcgacc gtgatcgtgc
     3961 ccttgagtga tgcaccgagc tgggccgtcg acggagagtc ctcgtcggag ctgccgacga
     4021 tcttcggctg cggaggagcg tccttgtcca tctgcgtgat ggccgtgtcg atccgcttgg
     4081 gcgcgccttc gcggaacgtc ggtgcggcgg ccgcgacggt gtcggcaccg acgatgatct
     4141 cgacgcgcgt gaacagctgg tcggcgtagt tacgctgacc gcgcttacct gcgacgagcg
     4201 ctgcctccat gagctccttc gccgtcgggt cggtgagctt tggcaagaga gccttcgctt
     4261 ttgcgagctt tgcgtcgctg atcggcttga gccgatccgc tggggtgatg atccgcggcg
     4321 cgagcgggcc gagtgcgccg agcagctcgc ccttctcctt tgcaggcacc ttcagctcgt
     4381 cgagcgcgag cgagagatcc tcgacgagag cattgaactc gtcctcgacg acctccatgc
     4441 ccgcgtgcga cgtctccatg tcctgaccga cgtagtggca aggaccgccc gtcgccatac
     4501 acacgaagtc gacgagcaac gacttcagcc gcgggatgtc ggtgttcgag aaccggatgt
     4561 tgatccgctt gtcggcggcg acgcgaccga tgaagccgtc gaccgcagca acgatcgctc
     4621 gttgaccacc gaggcggtcg tagaggtcag gtttcttcga tacggaggcc gccgccggtt
     4681 ttgcatcggc ggtcttggtt gtggttgtgg tttgcgcggt cggcttgccg ccgccaccgc
     4741 acgccatcag gacgcacgcg agtgttgctg cgagcttcat ggttgcctcc ggttcagaac
     4801 ggcgcggccg agtacgggaa cgtgctcgtc acgtcggggt ggtactggtt cgcgttggag
     4861 atgcccgtcg acgcgatggc gttgccggag ttcttcgcgt cgtcgctcga gaagttcacg
     4921 ccgtcgctga cgagcgccgt cacctggttg tacgagccct tcgctggcac cgcggcgccg
     4981 gcgagcaagt agttgtacgt gatgtcgatg acgtcctcgt tgagcaggcg accaccgcag
     5041 agcagcggca cgctgttcgc gtcaccgcag agcgtgaggt agccactcgg cgccgacgtg
     5101 tcgatgcgca tcacatcggg gatgaactgg ctgaacacct tgtcggcata cgcctgcgcg
     5161 gccgtcttcg attccggcgt gagcgtgaca ccagcgagcg tgttctcggt ccagatcgcg
     5221 gcgccaacgg cgtgacactg aatgcccgcc ggtttgagac ccgtcgacgg cgtcaggttc
     5281 agcgcgccat cgagcaagca cacgccgagg tacagcgcct tgagcacggt cttggcttcg
     5341 ccggtgatcg cggtaacggc ggcaggcggc gcaccggcaa agctcggcgc ggtcgcgttg
     5401 taaccggcgt tgaagtcgtt ggtgagcagc agcgcctcgt tgatgccggg tcgcgcgaga
     5461 tgctcgacct ggacatacgt gggcggctgg acgacggcag cgtcgacttc ctgcatgggc
     5521 gggccgtcgg ggacgatcgg attttcatcc ttgcagccgg cgacgaggaa caaaacaagg
     5581 gcgagtttct tcatggtgga tgctcctctc actgcttgac cgtgacgatg g
//
LOCUS       DATLBZ010000359         6453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1168248, whole genome shotgun sequence.
ACCESSION   DATLBZ010000359 DATLBZ010000000
VERSION     DATLBZ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168248"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..993)
                     /locus_tag="VMZ53_18505"
     CDS             complement(364..993)
                     /locus_tag="VMZ53_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30511.1"
                     /translation="MKRFLLLAVLAACGKPNDVKTLHHEAVVMAQYYQPQLDALDRRL
                     QAIFKRGTTIPGNLPGIEVVTPRLTEARDGIQQLRGIVGKPADGGKSAVEKQADLAAK
                     DRKVEELEKLIADTERTLNEGTTIIRDDLTTVENWIAQYEQGTNRVGGPQPSGAPGAE
                     GGQAPAGGQAPAGGGGQAPTPAPGNTTTPPTQNTTGQVPPAAAPAGHHQ"
     gene            complement(1029..3002)
                     /locus_tag="VMZ53_18510"
     CDS             complement(1029..3002)
                     /locus_tag="VMZ53_18510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IGHMBP2 family helicase"
                     /protein_id="HUS30512.1"
                     /translation="MSSANEWLDSLAAAWRAERLAARAKIAMERSGRSLVERVALGIA
                     LAHLRIVDEQSAPGERVRVRVAVPEQTDLDNLRLVPGEPVRLWAEHPDEDAAVRGVFE
                     RREEQSLWLMLDRPVDELDRDYALDPEAPELTFDRADAALEKARAALANSDLARLREV
                     AALARPGKLLAPTSWAPLDDELDEVQRAAVDVGLRSGDIALIWGPPGTGKTRTLVEVV
                     RQRVLRGERILCAAPSNTAVDNLGVRLAEAGVRAVRLGHPARVHPALTSLTIDAQVAR
                     DGAFDLAREWRDRARGIRKQAMGYDVRKGSREDMQRARAEVRGLWQEARALDRDAARE
                     INNAERAIIDRAQVVLATCVGCDHPMLGNAVFDCVIIDEATQAADPLLFIPLARAKVA
                     VLAGDPQQLGPVVIGGPSVEHVLGSTVFERLVEAGAPSVMLEQQHRMNDAIMQFPSTA
                     TYGGKLRASPAVAHHTLDDLGISPDAARGKPMWLVDTAGKDWLEERTDFDPGGSLNNM
                     PSFTFDPSTFNSGNAERVAAEARRLLSRGLAPTDLAIIAAYSAQARRLRDLLRDERAA
                     GVEVGTVDGFQGREKEAVIVDLVRSNDHGELGFLANTRRMNVALTRARRFLLVVADSA
                     TLGDHPYYARFIEYIDQIDGHGSAWSDEAEPLK"
     gene            3063..4928
                     /locus_tag="VMZ53_18515"
     CDS             3063..4928
                     /locus_tag="VMZ53_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HUS30513.1"
                     /translation="MPEATAPEKLTRKTTSIAQLFAQRVQQTPDREAYRYPQGENDWR
                     SMTWRQSGERVRAIAAGLLSLGLHREERVGILSNTRVEWLLCDLGILSAGGATTTVYP
                     SSTPEDCAFILADSDTRYCFVEDNSQVKKLRAHRAEMPKLVKLILLDGYPEAPKGSDG
                     DWVITLSELEQKGAEHLAKDPKAVDDAIAGIDSKHLATLIYTSGTTGKPKGVRLLHEC
                     WAYTSDAIGAVKLWGPDDVQYLWLPMSHSFGKVLMSGHIASGSVTAVDGRIPKIVENL
                     ATVRPTIMAAVPRIFEKVYNKILEGVKAGSPMKQRIFHWAVQVGKDVSKVRQQGREPT
                     GLLALKFRVADKLVFEKIKQRFGGRVRYFISGSAPLSREIAEFFHACGVLILEGYGLT
                     ESSAASFVNRPSKYAFGSVGMPMPGTELQLAKEDGEILMRSPGIMQGYHNLPDATAET
                     LTPDGWLKTGDIGEVDAHGFLRITDRKKDLIKTSGGKYIAPQSIEGKLKAACPYISQV
                     IVHGDKRNFVTALVTLDEEATMKWAKEKGFNGKPYSEVVNTKEARDLLDPYFKEVNKS
                     LAKYESVKNFAILPKDLSIEQGELTPSLKVKRTVVEKKYAPLLDKMYEGSIADAG"
     gene            4934..5545
                     /locus_tag="VMZ53_18520"
     CDS             4934..5545
                     /locus_tag="VMZ53_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30514.1"
                     /translation="MRTMLALMVVAACGKSSSPPENKETKATGSAPVGSAAAGSGSSA
                     AEPVEAATVSGPTKSATGTITVGGAITGSFQWVKKDQRAPITCVWDPDKEIGTLKIDL
                     SDGAGHVVTLGLDVPPTDVGPGRLEVISKELPSSLKTYRSFTMKPGDDADHFSVVFAG
                     TVAVSDPDAQTAEKPDKKKKAEPPPDPHVTLEGTLEMTCPKKK"
     gene            5561..6241
                     /locus_tag="VMZ53_18525"
     CDS             5561..6241
                     /locus_tag="VMZ53_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30515.1"
                     /translation="MRVTTWVVVAASLAACGKSDKKPAEPEKDKPALAGSGSSTAAST
                     TPNAGSNAGSNAGSAAVADARTVTGTLAVSGSLTGTFHWNSKGDLTCGWSAGKKAGAL
                     HLDLTDGIDHKTGGAAMEAGLLGDNAEPEPEGIGKDITMTVEFRAPVRLDLISVDLGA
                     PMKTEKGFEMSGPDDEHFKLVVDTTLTDVIVDPDAPKAKQKKGAKKPAGPSVTIKGTL
                     EITCLKKT"
     gene            complement(6219..>6453)
                     /locus_tag="VMZ53_18530"
     CDS             complement(6219..>6453)
                     /locus_tag="VMZ53_18530"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30516.1"
                     /translation="VTVLLYYATRISRAVLFWAAFILTRPLGAVVGDFLDKPRSQGGL
                     ALSRYTASLVLMAAMIVLIVVVPQRPFTSSSDT"
BASE COUNT         1161 a         2078 c         2197 g         1017 t
ORIGIN      
        1 ggggcgcctt cggcgcgagc agggcgtgtg caccaggcga gtcgtccggc acacgtcccc
       61 ccgtagcgtt tttctgcgtc cgctgctctc tcagcgactt tgcgttaact ctccgaaggc
      121 ggcgtcgaag acccgatgga acgcggggtc cgaagacccg gatggaacgc aaaggcgcaa
      181 tgaaggcaga gggcgcagaa gggaaggggc gccttcggcg cgagcagggc gtgtgcacca
      241 ggcaagtccc tccggcacac gtccccttaa ccctttttct gcgtcctctg ctctctcagc
      301 gtctttgcgt taggtctctg aacgctgtgc tcccgccgtg aaggcgtgac gcgaagagcg
      361 gcgctactgg tggtggccgg cgggggcagc ggccggcggg acttggccgg tcgtattctg
      421 cgtcggaggc gtcgtcgtgt tgccgggagc gggcgtcggc gcttggccac cgccaccggc
      481 gggtgcttgg ccaccggcgg gcgcttggcc gccctcggcg ccgggcgcgc cggacggctg
      541 cgggccaccc acgcggttcg tgccttgctc gtattgggcg atccagttct cgacggtggt
      601 gaggtcgtcg cgaatgatcg tcgttccctc attgagcgtt cgctcggtgt cggcgatcag
      661 cttctcgagc tcttcgacct tgcggtcctt cgctgcgaga tcggcctgct tctcgacggc
      721 gctcttgccg ccgtcggcgg gtttgccgac gatgccgcgc agctgctgga tgccgtcgcg
      781 ggcttccgtc aggcgcggcg tgacgacctc gatgccgggg aggttgcccg ggatcgtggt
      841 gccacgcttg aagatcgctt ggaggcggcg atcgagcgcg tcgagctgag gctggtagta
      901 ctgcgccatc acgacggctt catgatgaag cgtcttgacg tcattcggct tgccacaggc
      961 tgcgaggacg gcgaggagga gaaagcgctt catcataacg acggagcgtt catctcatcg
     1021 ttacggcttc acttcaaggg ctcggcctcg tcactccatg cgctgccgtg tccatcgatc
     1081 tgatcgatgt actcgatgaa ccgtgcgtaa taggggtgat cgccgagcgt ggcggagtcc
     1141 gcgacgacga ggaggaagcg gcgagcgcgg gtgagcgcga cgttcatgcg gcgcgtattc
     1201 gcgaggaagc cgagctcgcc gtgatcgttg ctgcggacga gatcgacgat gaccgcctct
     1261 ttttcgcgac cctgaaagcc gtcgaccgtg ccgacttcga cgcccgcggc gcgctcgtcg
     1321 cggagcaggt cacgcaggcg acgcgcttgc gcggagtacg ccgcgatgat ggcgaggtcg
     1381 gttggcgcga ggccgcgcga gaggaggcga cgcgcttcgg cagcgacacg ctcggcattg
     1441 ccgctgttga acgtcgacgg atcgaacgtg aacgacggca tgttgttgag cgagccaccg
     1501 ggatcgaagt cggtgcgctc ctcgagccaa tccttgccgg cggtgtcgac gagccacatc
     1561 ggcttgccgc gcgcagcgtc gggcgagatg cccaggtcat cgagcgtgtg atgcgcgacc
     1621 gcgggcgatg cgcgcagctt gccgccgtat gtcgccgtcg acgggaactg catgatcgca
     1681 tcgttcatgc ggtgctgttg ctcgagcatc accgacggcg cgccagcctc cactagccgt
     1741 tcgaacaccg tcgagccgag cacgtgctcc acgctcgggc cgccgatgac cacggggcca
     1801 agctgctgcg gatcgccggc gagaaccgcg accttcgcgc gcgcgagcgg gatgaagagc
     1861 agcgggtcgg cggcttgcgt cgcctcgtcg atgatcacgc agtcgaacac ggcgttgccc
     1921 agcatcgggt ggtcgcaacc gacgcacgtg gcgagcacga cttgggcgcg gtcgatgatc
     1981 gcgcgctccg cattgttgat ctcgcgtgcc gcatcccgat cgagcgctcg cgcttcttgc
     2041 cagaggccac ggacttcggc gcgtgcgcgc tgcatgtcct cgcggctgcc tttccgcacg
     2101 tcgtagccca tcgcttgctt gcggatgccg cgagcgcggt cgcgccattc acgcgcgaga
     2161 tcgaacgcgc catcgcgagc gacctgcgca tcgatcgtca gcgaggtgag ggcagggtgc
     2221 acgcgcgcgg gatggccgag ccgaaccgcc cgcacgcccg cttcggcgag ccgaacaccg
     2281 agattatcga cggccgtgtt cgacggcgcg gcgcacaaga tgcgctcacc gcgcagcacg
     2341 cgctggcgca cgacctcgac gagcgttcgc gtcttgccgg tacccggcgg cccccagatg
     2401 agcgcgatgt caccgctgcg caggccgacg tcgaccgcgg cgcgctgcac ctcatcgagc
     2461 tcgtcgtcga gcggcgccca gctcgtcggc gcgagcagct tgcccgggcg cgcgagcgca
     2521 gcgacttcgc gaaggcgcgc gagatccgaa tttgcgagcg ccgcgcgtgc cttctccagg
     2581 gccgcgtccg cgcgatcgaa cgtcagctcg ggagcctcgg gatcgagcgc gtagtcacgg
     2641 tcgagctcgt cgaccggccg gtcgagcatc agccacaggc tctgctcttc gcgccgttcg
     2701 aagacgcctc gcactgcggc gtcctcgtcg ggatgctcgg cccacaggcg caccggctcg
     2761 cccggaacga ggcgcaagtt gtcgaggtcg gtctgctcgg ggacggcaac gcggacgcgc
     2821 acgcgctctc ccggggccga ttgctcgtcg acgattcgca ggtgagcgag ggcaattccg
     2881 agcgcgactc tctcgacgag cgaccgtccg ctccgctcca tggcgatctt ggcccgcgcg
     2941 gccagccgtt ccgcccgcca ggcggcggcg agcgagtcca gccattcgtt cgcggacgac
     3001 atgggaccgg aagggtaccg ccgatccctt ccgagaggaa ttgcggcata ttcttccccg
     3061 ccatgcccga agccaccgcc ccagagaagc tcacgcgcaa gaccacgtcc atcgcgcagc
     3121 tctttgcgca gcgcgtccag cagaccccgg atcgcgaagc gtatcgctat ccgcaaggcg
     3181 agaacgactg gcgctcgatg acgtggcgcc aaagtggtga gcgcgtgaga gcgatcgccg
     3241 cgggcctgct ctcgctcggc ctgcatcgtg aggagcgcgt cggcattctc agcaacacgc
     3301 gcgtcgagtg gctgctctgc gacctcggca tcttgtcggc aggtggcgca acaacgacgg
     3361 tctacccgtc gagcacgccc gaagactgcg cgttcatcct cgccgacagc gatacccgtt
     3421 attgctttgt cgaggacaac agtcaggtca agaagctccg cgctcaccgg gccgagatgc
     3481 ccaagctcgt gaagctcatc ctgctcgatg gctatcccga ggcgccgaag ggcagcgatg
     3541 gcgactgggt gatcacgctg agcgagctcg agcagaaggg cgccgagcac ctcgcaaaag
     3601 atccgaaggc cgtcgacgac gcgatcgcag gcatcgacag caagcacctc gcgacgctca
     3661 tctatacgtc cggcaccacg ggcaagccaa agggcgtgcg cctcctccac gagtgttggg
     3721 cgtacacgtc cgatgcgatc ggcgccgtga agctgtgggg ccccgacgac gtccagtacc
     3781 tctggctccc gatgagccac tcgttcggca aggtgctgat gagcggccac atcgcgtcgg
     3841 gctcggtcac cgccgtcgat ggacgcatcc cgaagatcgt cgagaacctg gcgacggtgc
     3901 gcccgacgat catggccgcc gtgccgcgca tcttcgagaa ggtctacaac aagatcctcg
     3961 agggcgtgaa agccggctcg ccaatgaagc agcgcatctt ccactgggcg gtgcaggtcg
     4021 gcaaagacgt cagcaaggtg cgccagcaag gccgcgagcc caccggcttg ctcgcgctca
     4081 agttccgcgt cgccgacaag ctcgtgttcg agaagatcaa gcagcgcttc ggcggtcgcg
     4141 tgcgctactt catctcgggc tcggcgccgc tgtcgcgcga gatcgccgag ttcttccacg
     4201 cgtgcggcgt cctgatcctc gagggctacg gcctcaccga gtcgagcgcg gcaagcttcg
     4261 tgaatcgtcc gagcaagtac gcgttcggca gcgtcggcat gccgatgccc ggcaccgagc
     4321 tgcagctcgc aaaagaggac ggcgagatcc tcatgcgctc gcccggaatc atgcagggct
     4381 atcacaacct gcccgacgca accgccgaga cgctcacgcc ggatggctgg ctcaagacgg
     4441 gtgacatcgg cgaggtcgac gcgcacggct tcctgcgcat cacggatcgc aagaaggacc
     4501 tgatcaagac gagcggcggc aagtacatcg cgccgcagtc gatcgagggc aagctcaagg
     4561 ccgcgtgccc gtacatctcg caagtgatcg tccacggtga caagcgcaac ttcgtcaccg
     4621 cgctcgtgac gctcgacgaa gaagcgacga tgaagtgggc gaaggagaag ggcttcaacg
     4681 gcaagcctta ctccgaagtc gtcaacacaa aagaagcccg cgacctgctc gatccgtact
     4741 tcaaggaggt caacaagagc ctcgcgaagt acgagagcgt gaagaacttc gcgatccttc
     4801 cgaaggatct gtcgatcgag caaggcgagc tcacgccgag cctcaaggtg aagcgcacgg
     4861 tcgtcgagaa aaagtacgcg ccgctgctcg acaagatgta cgagggctcg atcgcggacg
     4921 cggggtgatg gtcatgcgca caatgcttgc gttgatggtg gtggccgcgt gcgggaagag
     4981 ctcgtcgccg ccggagaaca aagagacgaa ggccaccggc agcgcgcccg tcggatctgc
     5041 agcggcaggc tcgggctcgt cagctgccga accggtcgaa gcggcgaccg tgagcggtcc
     5101 gacgaagtcg gccacgggca cgattacggt cggcggcgcg atcacgggct cgttccaatg
     5161 ggtcaagaag gatcagcgcg cgccgatcac gtgtgtctgg gatccggaca aagaaatcgg
     5221 gacgctcaag atcgatctca gcgacggcgc gggtcacgtg gtgacgctcg gtctcgatgt
     5281 cccgccgacg gacgtcgggc ccggccgcct cgaagtcatc tcgaaggagc tgccgtcgtc
     5341 gctgaagacg taccggagct tcacgatgaa gcccggcgac gacgcggatc acttcagcgt
     5401 ggtcttcgcg ggcaccgtgg cggtctctga cccagacgct caaaccgcgg agaagccgga
     5461 caagaagaag aaagcagaac cgccgcccga tccgcacgtc acgctcgaag gcacgctcga
     5521 gatgacctgt ccgaagaaaa aatgatgcga tgatgtcgtc atgcgcgtga cgacatgggt
     5581 agtcgtagcg gcgagcctcg ccgcgtgtgg gaagagcgac aagaagccgg cggagcccga
     5641 gaaggacaag ccggcactcg ctggttcggg ctcgagcact gctgcgagca cgacgccaaa
     5701 tgccggctcg aacgcgggct cgaacgctgg atcagcggcg gtcgcagacg ctcgtaccgt
     5761 gaccgggacc cttgcggtca gcgggtcgct caccggcacg tttcactgga actcgaaagg
     5821 tgacctcacc tgcggatgga gcgcgggcaa gaaggcgggc gccctccatc tcgatctcac
     5881 cgacggcatc gatcacaaga cgggtggcgc cgcgatggag gcggggctct tgggtgacaa
     5941 cgcggagccc gagcccgaag gcatcggcaa ggacatcacg atgacagtcg aattccgtgc
     6001 gccggtgcgg ctcgatctga tctccgtcga tctgggcgct ccgatgaaga cggagaaagg
     6061 cttcgagatg tcggggcccg atgacgagca cttcaagctc gtcgtcgaca cgacgctcac
     6121 cgacgtcatc gtcgacccag acgcgccgaa ggccaagcaa aagaagggcg cgaagaagcc
     6181 ggccggccct tcggtcacga tcaagggcac gctcgagatt acgtgtctga agaagacgtg
     6241 aacggtcgct gcggcacgac gacgatcaag acgatcatcg ctgccatgag gacgagcgac
     6301 gcggtgtagc ggctcagcgc gagcccgcct tggctccgcg gtttgtcgag gaagtcgccg
     6361 accaccgcac cgagcggtcg cgtgagaata aacgccgccc agaacaagac cgcgcgcgaa
     6421 attcgcgtgg cgtagtagag caagacggtc act
//
LOCUS       DATLBZ010000360         6668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9360925, whole genome shotgun sequence.
ACCESSION   DATLBZ010000360 DATLBZ010000000
VERSION     DATLBZ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9360925"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2401)
                     /locus_tag="VMZ53_18535"
     CDS             complement(<1..2401)
                     /locus_tag="VMZ53_18535"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUS30517.1"
                     /translation="MWISNYAIKKPIITTVVMVALVVFGFIALMRLKTDEFPEVTAPV
                     VNIAIIYPGASPGVVERELIDPLEESFRSISGVDTIQSTAVDGYANILVVFTFEKDIQ
                     QATQDIRDKISESRADLPIEMEEPILTRFASTDFPIISLTLTSTSTPAVTLTQLADVK
                     IRRELTALGGVASVDVVGGVERELTVEVKPDALRAKGIAIGQVVQTLQAENLAVPVGK
                     LSGKLEDRTIRLRGRLTSPSEFEQLVVGRNGDKVVRLGDVADVKDGEEDAISAAFYNT
                     TPAVGIDIKKALGYSTTDVSERVLKRIKELEKELPPGTKMEVVKDSGARVEASVSDVQ
                     SSLFEGALLTILVVLIFLKSWRSTVITGLALPVSVIASFVAVLAWGFTLNVMSLLGLS
                     LAIGILVDDAIVVRENIVRHMEMGKDHVQAAREGTSEIGLAVAATTFSIVVVFVPIAF
                     MGGIAQQWFAPFALTIACSVMVSLFVSFSLDPMLSAVWHDPDATGEHRSWLSRKLDIF
                     DRLFQRLTRVYKRVISWALRHRFLMVVLALAAFFGALAIPASGAVGSGFFPVEDRSEF
                     QITLETSPGSNLAYTKTKVVAAVELARKHPEVMYAYSTVGGQTGADTAQIYVRLTPKK
                     ARSVHQDVVAQSLRNEIRQLSGVTAYITTGFGGGFRQIQIQLIGPDTDVLNGIAMRVA
                     NEVRKVPGAVDVGLSSKGQRPEVEVLPDRDLTGQLGVSAATLAQAVRPAFAGLDAGRW
                     IDPDGETRDVVVRLAADWRERPEDLEALPINLPPTGADPPKPVLLGQIAKVKRGFGPA
                     QI"
     gene            complement(2402..3547)
                     /locus_tag="VMZ53_18540"
     CDS             complement(2402..3547)
                     /locus_tag="VMZ53_18540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUS30518.1"
                     /translation="MRSLLIVLLALVVGCKKPPEQAPAQAEALPIRLAKEDIVTIQRG
                     ELESGPRISGTLQAAARAVVRAETPGSVVAIGPELGQLVKKGDLLARVEAKALGDVTT
                     SAQSGVTAAQAQLELAQREVQRTEALVKGGALAQRELDRAQSQVTAAQAQVTQARAQL
                     ASSKSQLGDATVRAPISGVVAKRAVNTGDVVSPGSELYEVIDPSTMRLDASVASDDLS
                     VIGVGKAVDFEVRGYPDQRFAGTIARIAPAADPVTRQIQVLVDIPNPGGKLIAGLYAE
                     GRVSVNKREALIAPMSAIDASGDQPSVLRVKNGVVERTIVAIGVRDERAEVVEVTSGL
                     APGDVLLLARATKNVAVGAKVEIPGNSAPPPAAKPQAGSGSAGSGAK"
     gene            complement(3556..4941)
                     /locus_tag="VMZ53_18545"
     CDS             complement(3556..4941)
                     /locus_tag="VMZ53_18545"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUS30519.1"
                     /translation="MIRFLIVACVAAVAFGYARADDTPSSAQSLSLEDALHAAEKASE
                     TLAVARADLDRAQTQVTSARSGYLPTINGTAAYQRTLASEFDDINFGFGMGSGSGSGS
                     AAPDIELPFGQRNNWRLGLQAQQPLFDGFRTAAAIEQAKAGVRVSELGVQATRAQLVL
                     QVAVAYYDAALADRQVEIAEVTLQQSEATLKETELKYKQGTAPEFDLVRAQVARDNQS
                     NALVQFRVQRDFAFVQLRRLVGVPLDRDVTLSTKLEANDVDGMVELARGAAGLPPSNT
                     RVVVAQAKEGIAAREAGVKLAKSAWYPTLSAGTDFGLVNYQNQPFNSDWRTNWTLGVT
                     LSLPLFDGFRRVASQRASEAELASARAQAANATEVAQVEAAQAAASVAASAQTLETSA
                     RTVKQAQRAYQIAELRFQQGASTHLELVDARVQLEQAQLNEARSSRDLRVARLRQALL
                     PGLPLGGAAGF"
     gene            5051..5917
                     /locus_tag="VMZ53_18550"
     CDS             5051..5917
                     /locus_tag="VMZ53_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30520.1"
                     /translation="MRLALLAILCLTTTAFAETPQERAKAFVTTQLNAIVKNDADAFK
                     ATFSADAVLLASGAYEPIATRENLEFQQMLMSGSPHDVFKKAKLDKIVAGGDANVIWL
                     TAELATTYDAYDEETPPKKNQTARTRLTELLVADGTTWKAVAASLAQPRSSSGTPEDP
                     SHTMVESATKPGPLAPLVANPTELIKKLSADKNVFVLGTDKNERAVGQTAAKKLLTGW
                     SKLKLTIDEKSVREVATKTYAFAQVSVTYTTPKKVSGKAVTLYHPMTAIIMAVPSGSD
                     WSVVGVNYIADR"
     gene            complement(5919..>6668)
                     /gene="guaA"
                     /locus_tag="VMZ53_18555"
     CDS             complement(5919..>6668)
                     /gene="guaA"
                     /locus_tag="VMZ53_18555"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HUS30521.1"
                     /translation="IFRDHFHVDLRVIDAEERFLTQLKGVTDPEKKRKIIGYEFIAVF
                     EEQAKSIKNAKFLAQGTLYPDVIESVSFKGPSHTIKSHHNVGGLPERMQLALVEPLRE
                     LFKDEVRQLGIELGLPRHMVMRQPFPGPGLAVRCLGNLERARLDVLRNADAIIEEEIR
                     SAGLYESIWQSFGVLLPVKSVGVMGDARTYEETLAVRAVNSRDGMTADWVQLPYDVLA
                     RISSRIINEVRGINRVVYDITSKPPGTIEWE"
BASE COUNT         1156 a         2203 c         2197 g         1112 t
ORIGIN      
        1 cgatctgcgc tgggccgaag cctcgtttca ccttcgcgat ttggccgagc agcacgggct
       61 tcggtgggtc ggcgcctgtt ggcgggaggt tgatcggcag cgcttcgaga tcttccgggc
      121 gttcgcgcca gtcggcagcg aggcggacga cgacgtcgcg cgtctcaccg tcgggatcga
      181 tccagcggcc ggcgtcgagg ccggcgaatg cggggcggac ggcttgcgcg agcgtcgccg
      241 cagagacgcc gagctgcccc gtcagatcgc gatcggggag gacttcgact tcggggcgct
      301 ggcctttgct ggagaggccg acgtcgacgg cgcctgggac cttgcggact tcgttcgcga
      361 cgcgcatcgc gatgccgttg aggacgtcgg tgtcgggacc gatgagctgg atctggatct
      421 gacggaagcc gccgccgaag cccgtcgtga tgtacgcggt cacgccgctg agctggcgga
      481 tctcgttgcg cagcgattgc gcgacgacgt cttggtgaac actgcgcgcc ttcttcggcg
      541 tgaggcgcac gtagatctgt gcggtgtcgg cgccggtttg cccgccgacg gtggagtacg
      601 cgtacatgac ctcgggatgc ttgcgcgcga gctcgaccgc cgcgacgacc ttcgtcttcg
      661 tgtacgcgag gttgctgccg ggtgaggtct cgagcgtgat ctggaattcg gagcggtctt
      721 ccacggggaa gaagccactg ccgactgcgc cgctggccgg gatcgcgagc gcgccgaaga
      781 atgcggcgag cgcgagcacg accatcaaga agcggtggcg cagcgcccag ctgatgacgc
      841 gcttgtagac gcgcgttagg cgctggaaca ggcgatcgaa gatgtcgagc ttgcgcgaga
      901 gccaggagcg atgctcgccc gtcgcgtcgg ggtcgtgcca gaccgccgag agcatcgggt
      961 cgagcgagaa cgagacgaac agcgagacca tcacggagca cgcgatcgtc agcgcgaacg
     1021 gggcgaacca ttgttgtgca atgccgccca tgaatgcgat cgggacgaac acgacgacga
     1081 tcgagaacgt ggtcgcggcg accgcgagcc cgatttcgga cgtgccttcg cgcgcggctt
     1141 ggacgtggtc cttgcccatc tccatgtggc gcacgatgtt ttcgcgaacg acgattgcgt
     1201 cgtcgacgag gatgccgatc gcgagcgaga ggccgagcag cgacatcacg ttgagcgtga
     1261 aaccccacgc gagcaccgcg acgaatgacg cgatgacgga cacgggcagc gcgaggccgg
     1321 tgatcaccgt cgagcgccac gacttgagaa agatgagcac gacgaggatc gtgagcagcg
     1381 cgccttcgaa cagcgagctc tgcacgtcgc tgaccgaggc ttcgacgcgc gcgcccgagt
     1441 ctttgacgac ctccatcttc gtgccgggcg gcagctcctt ctcgagctcc ttgatccgct
     1501 tgaggacgcg ttcgctgacg tccgtcgtcg agtaaccgag cgccttcttg atatcgatgc
     1561 caacggcggg cgtcgtgttg tagaacgcag cgctgatcgc atcctcttcg ccatctttga
     1621 cgtcggcgac atcgccgagg cgaacgacct tgtcgccgtt gcgaccgacg acgagctgct
     1681 cgaactcact cgggctcgtg agacgcccgc gcaggcgaat cgtgcggtct tcgagcttgc
     1741 ccgagagctt gccgacggga acggcgaggt tctcggcttg cagcgtctgg acgacttgac
     1801 cgatcgcgat gccctttgca cgcagcgcat cgggtttgac ctcgaccgtg agctcgcgct
     1861 cgacaccgcc gaccacgtcg acactcgcga cacccccgag ggcggtcagc tcacggcgaa
     1921 tcttgacgtc ggcgagctgg gtgagcgtga cggctggcgt cgaggtggac gtgagcgtca
     1981 gcgagatgat cggaaagtcc gtcgacgcga agcgcgtcag gatcggctct tccatttcaa
     2041 tcggcaggtc ggcgcgcgac tccgagatct tgtcgcggat gtcctgcgtc gcctgttgga
     2101 tgtccttctc gaacgtgaag acgacgagga tgttcgcgta gccgtcgacg gcggtggact
     2161 ggatcgtatc gacgcccgag atcgaccgaa acgattcttc gagcggatcg atcagctcgc
     2221 gctcgacgac gccaggcgac gcgcccgggt agatgatcgc gatattgacg accggcgccg
     2281 tcacctccgg gaactcgtcg gtcttcagcc gcatcagcgc gatgaagccg aacacgacga
     2341 gcgcgaccat cacgaccgtc gtgatgatcg gcttcttgat cgcgtagttc gagatccaca
     2401 tctacttggc tcctgaaccc gctgatccgg aacccgcctg cggcttggct gccggcggtg
     2461 gtgccgagtt gccaggaatc tcgaccttcg cgccgaccgc aacattcttc gtcgcgcgcg
     2521 cgagcagcag cacatcgcct ggcgcgaggc ccgacgtcac ctcgacgacc tcggcacgct
     2581 catcgcgaac accgatcgcg acgatcgtgc gctcgacgac gccgttcttg acgcgcagca
     2641 cgctcggctg atcgccgctc gcgtcgatcg cggacatcgg cgcgatcaac gcctcgcgct
     2701 tgttgaccga caccctgccc tctgcgtaga ggcccgcgat cagcttgccg ccgggattgg
     2761 ggatatcgac gagaacctgg atctgacgcg tcaccggatc ggcggccggc gcgatccgcg
     2821 cgatggtgcc ggcgaaccgc tgatcgggat agccgcgcac ttcgaagtcg acggccttgc
     2881 cgacaccgat caccgagagg tcgtccgacg ctaccgaggc atcgagccgc atcgtcgacg
     2941 gatcgatcac ctcgtacagc tcggacccgg gcgacacgac atcacccgtg ttcaccgcgc
     3001 gcttcgcgac gacgcccgag atcggcgcac gcaccgtcgc gtcaccgagc tggctcttcg
     3061 acgacgcgag ctgcgcgcgg gcctgcgtga cctgcgcttg ggccgcggtg acctgactct
     3121 gcgcgcggtc gagctcgcgc tgggccaacg ccccgccctt cacgagagcc tcggtgcgct
     3181 ggacttcgcg ctgcgcgagc tcgagctgag cctgcgcggc cgtcacgccg gactgcgccg
     3241 acgtcgtgac atcgccgagc gccttcgctt cgacgcgcgc gagcaggtcg cccttcttca
     3301 cgagctggcc gagctcgggc ccgatcgcga cgaccgagcc gggcgtctct gcgcgcacga
     3361 ccgcacgcgc ggcggcctgc agcgtgcccg agatgcgcgg cccgctctcg agctcaccgc
     3421 gttggatcgt gacgatgtct tccttcgcga gcctgatcgg cagcgcttcg gcttgagcgg
     3481 gtgcttgctc gggcggcttc ttgcaaccga cgacgagcgc gagcagaaca atcaacaagc
     3541 tacgcatcga gaacctcaga acccggccgc gccgccgagc ggcaacccgg gcaaaagcgc
     3601 ctgacgcagg cgcgcgacac gcaaatcgcg cgacgaccgc gcttcgttga gctgagcctg
     3661 ttcgagctgg acgcgcgcgt cgacgagctc gaggtgcgtc gaggcgcctt gttgaaagcg
     3721 aagctcggca atttgatagg cgcgctgcgc ttgcttgacc gttcgcgcgc tcgtctcgag
     3781 cgtctgcgcg ctcgccgcca ccgacgccgc cgcctgtgcg gcctcgactt gcgcgacctc
     3841 ggtcgcattc gctgcttgcg cacgcgccga tgcgagctcc gcctccgacg cacgttggct
     3901 cgcgacgcga cggaagccgt cgaagagcgg cagcgacagc gtcacgccga gcgtccagtt
     3961 cgtgcgccag tccgagttga acggctgatt ctgatagttg acgagaccga agtcggtgcc
     4021 cgcggacagc gtcggatacc acgccgactt cgcgagcttc acgcccgcct cgcgtgctgc
     4081 gatgccctcc ttcgcctgcg cgacgacgac acgtgtgttg gacggcggaa gccccgccgc
     4141 gcctcgcgcg agctcgacca tcccatcgac gtcgtttgct tcgagcttcg tcgagagcgt
     4201 gacatcgcga tcgagcggca caccgacgag ccggcgcagc tgcacgaacg cgaagtcgcg
     4261 ctgcacgcga aactggacga gcgcgttgct ctggttgtca cgcgcgacct gcgcacgcac
     4321 gagatcgaac tcgggcgccg tgccttgctt gtacttgagc tcggtctcct tcagcgtcgc
     4381 ctcggattgc tgcagggtga cctcggcgat ctcgacttgg cgatcggcga gcgcagcgtc
     4441 gtagtacgcg acggcaactt ggagcacgag ctgcgcacgc gtcgcctgca cgccgagctc
     4501 cgacacgcgc acgcctgctt tcgcttgctc gatcgctgcc gcggtgcgga agccgtcgaa
     4561 tagcggctgc tgcgcctgca ggccgaggcg ccagttgttg cgctggccaa atggcagctc
     4621 gatatccggt gccgcgctgc ccgatccgct gcccgacccc atgccgaaac caaaattgat
     4681 gtcgtcgaac tccgaggcga gcgtgcgctg atacgcggcc gtgccgttga tcgtcggcag
     4741 gtagccgctt cgcgccgacg tcacctgcgt ctgcgcgcga tcgaggtccg cgcgtgccac
     4801 cgccagcgtc tccgacgcct tctcggcggc gtgcagcgca tcttcgagag agagcgactg
     4861 cgcgctcgat ggagtgtcat cggctcgtgc gtatccgaac gcaaccgccg caacacacgc
     4921 cacgatcagg aaccgaatca tggggccttg gcggaccctt ccattgaacg ggcaccccag
     4981 caaccatccg agcgcaccaa tccgcgcaac agatcgtttg tacagactcc cgacaggcat
     5041 actgtaagcc atgcgtctcg cgttactcgc gatcctctgc ctcacgacca ccgccttcgc
     5101 cgaaacgccg caagagcgcg ccaaggcgtt tgtcacgacc cagctcaacg cgatcgtcaa
     5161 gaacgacgcc gatgcgttca aagcgacatt cagcgccgat gccgtcctgc tcgccagcgg
     5221 cgcgtacgag ccgatcgcca cgcgcgagaa cctcgagttt cagcaaatgc tgatgagtgg
     5281 cagcccgcac gacgtcttca agaaggcgaa gctcgataag atcgtcgcgg gcggcgacgc
     5341 caacgtgatc tggctgaccg ccgagctcgc gacgacgtac gacgcgtacg acgaagaaac
     5401 gccgccgaag aagaaccaga ccgcgcgcac gcgcctgacc gagctgctcg tcgccgatgg
     5461 cacgacgtgg aaggcggtcg ccgcgtcact cgcacagccg cgctcgtcgt cgggcacgcc
     5521 cgaggatccg agtcacacga tggtcgagag cgccacgaag cccgggccac tcgcgccgct
     5581 cgtcgcaaac ccgacggagc tgatcaagaa gctgtcggcg gacaagaacg tgttcgtgct
     5641 cggcaccgac aagaacgagc gcgcggtcgg tcagacagcc gcaaagaagc tgctgactgg
     5701 gtggtcgaag ctcaagctga ccatcgacga gaaatcagtc cgcgaggtcg cgacgaagac
     5761 gtacgccttt gcgcaggtca gcgtcacgta caccacgccg aagaaggtga gcggcaaagc
     5821 cgtcacgctc tatcacccga tgacggcgat catcatggcg gtgccaagcg gcagcgactg
     5881 gagcgtcgtc ggcgtgaatt acatcgccga caggtgagct attcccactc gatcgttccg
     5941 ggcggcttgc tcgtgatgtc gtagacgacg cggttgatgc cgcgcacttc attgatgatg
     6001 cgcgagctga tccgcgcgag cacgtcgtac ggcagctgca cccaatcggc agtcatgccg
     6061 tcgcgcgaat tgacggcgcg caccgcgagc gtctcttcat acgtgcgcgc atcgcccatc
     6121 acaccgacgg acttcacggg aagcaggacg ccgaacgact gccagatcga ctcgtagagt
     6181 cccgccgaac ggatttcttc ttcgatgatc gcgtcggcgt tgcgcaggac gtcgagccgg
     6241 gcgcgctcga ggttgccgag gcagcgcacc gcgaggcccg ggccggggaa cggctggcgc
     6301 atcaccatgt gacgcggcag accgagctcg atgccgagct gacggacttc atctttgaac
     6361 agctcgcgaa gcggctcgac gagtgcgagc tgcatgcgct cgggcaatcc accgacgttg
     6421 tggtggctct tgatcgtgtg cgacggaccc ttgaacgaga cgctctcgat gacgtctgga
     6481 tacagcgtgc cctgcgcgag gaacttggcg ttcttgatgc tcttggcctg ctcctcgaac
     6541 acggcgatga actcgtagcc gatgatcttt cgcttcttct ccggatcggt cacgcccttg
     6601 agctgcgtga ggaaccgctc ttcggcgtcg atcacgcgga ggtcgacgtg aaagtgatcg
     6661 cggaagat
//
LOCUS       DATLBZ010000361         4637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4127536, whole genome shotgun sequence.
ACCESSION   DATLBZ010000361 DATLBZ010000000
VERSION     DATLBZ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4127536"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1821)
                     /locus_tag="VMZ53_18560"
     CDS             complement(178..1821)
                     /locus_tag="VMZ53_18560"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUS30522.1"
                     /translation="MMPVGKLTRMVAANTLRSPRHFFLSAFGIVIGIATFVVFLASTE
                     QIGAVLEKIFPLEQVQVVAPRASLLGKDISKKLDDNIVKTILARPEVKAAVPRMSLTF
                     PAFGRGSFEGSDLRFEVGGFADGVDASYVQDDERIRELFKDWMADDKDPHRVACMPPP
                     KSDEDEVIQSPKAPPAGRQTKLGQTPPTEGPPQPTPPAAPPPADPATGSAIPILPQAA
                     PAQFDWRAGEFWVVGKKGEYLNPCPEPERYYCDEESRRCEHRVPIVMSPTMIELYNGQ
                     FAKSHGLPIIETDFVKWVTQRGGLGQMRFSIGLGQTTVAGSNTAIQHRPRRVEAVLVG
                     MSNRAMPIGMTVPIQYISRWNRQYQGEEAATTYSSIIVTLKNRDDIAPFSQWLQDDLD
                     LRLEDSLGEKFATALFVIRFALIGISLVIIGISAINIAHNFFMQVTERRRELGLLRAL
                     GATKADVRWVVLGEAALIGLIGGILGVLLGMLMAKGVDYASAHWAPRFPYKPATWFNF
                     KWWIWTGGLACAAGFAVLGGYLPARRASSMEPAQALTQN"
     gene            complement(1824..2537)
                     /locus_tag="VMZ53_18565"
     CDS             complement(1824..2537)
                     /locus_tag="VMZ53_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS30523.1"
                     /translation="MASPVVRLRDVTKSYGEGTAKTQVLRGVSFDIEKGELIALVGQS
                     GSGKSTLLNIIGGLDTCDAGEVEVLGVDTRKASDAKLAQMRNESMGFVFQAFNLMDHL
                     TVLQNVTLPAAFSKNSKDVEGRAKDSLRRVGLSDFASRYPGELSGGQKQRVAIARALF
                     GSPSLLLCDEPTGNLDSETGREVIDFFRELNAKDGVTLLIVTHERRISSVAKRVIAMR
                     DGMLVETTDEALAASAGPV"
     gene            complement(2562..4019)
                     /locus_tag="VMZ53_18570"
     CDS             complement(2562..4019)
                     /locus_tag="VMZ53_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30524.1"
                     /translation="MRVVFLIALAVAACGGSQKGSGGGGGGGAEVFVGARFVPPRPTY
                     VVAAPTFRSAQLAFHDAADIFGMAVGGEVEEIGRALQVLLGIDPLNPDAVAQIGVDMN
                     GALVAFSEDIDPTFVVHLADPKAMAAYLDGQRAQGMKLTSMIVDNVEVFTARIEGTVQ
                     VSWAIDKDWLWVHFADMNAELGTWFEHSHKAGAPTWTQAWDWAKAQASGQGLTGFANL
                     RDAFAVITKRVPEAAACARQFESVARVGVSVTADGRTLDGHLSFDIGGSAQNIGGALL
                     QPPPGWTGASQGMPVSAQWNLDAAFMQRWMAPCMQREASDVAAMIDQFGVRSARGLVQ
                     QFDPDDKSGVGAVSLDLTHSRFIAEQLDQVPMRSRLEKDRTYGQYKGKHLSVPFVATV
                     DYVLTNTLFIGAMGDGLLAKLGSGAPQGGPPPIASVDVLPAGLKPDVWQWLFEQIGAP
                     SPQRLAQRLQNWQNLHIGARLDGTSLVIEANGTRR"
     gene            4131..4460
                     /locus_tag="VMZ53_18575"
     CDS             4131..4460
                     /locus_tag="VMZ53_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30525.1"
                     /translation="MSPIGGAGGPGGVGGPKGPDGPDDVDDVADVGSETSVSSTTAAK
                     AAGGLEAMASEIAAGRLTPREAVDKLVDEIAGTDQLGAGERAELRELLSDLVENDPYL
                     ASLVGRA"
BASE COUNT          795 a         1547 c         1487 g          808 t
ORIGIN      
        1 gaggaggtag tactttccga aaggcgtcgg tttcttcacg cgtttactcg cagcggcgat
       61 catcgctccg tcgctgctag atgatgggac cctatccgaa ttcgattcct tgggccacaa
      121 gctctggtta cccccgatgg gcggtagtag tagcttggag atcccttgga ttcctagcta
      181 attctgcgtc agtgcttgcg caggttccat cgaagaagca cggcgcgccg ggaggtagcc
      241 gccgagcacc gcgaagcctg ccgcgcaggc tagccctccg gtccagatcc accacttgaa
      301 gttgaaccag gtcgcgggtt tatacgggaa tcggggcgcc caatgggcgg acgcatagtc
      361 gacgcctttg gccatgagca tgccgaggag cacacccaag atgccgccta tcaggccaat
      421 cagcgccgct tcgccgagga cgacccaccg aacgtcggcc ttcgtggcgc ccagcgcgcg
      481 gagcaaccca agctcgcgcc gccgctcggt gacctgcatg aagaagttgt gggcgatgtt
      541 gatcgcgctg atgccgatga tgacgagcga gatgccgatg agcgcgaagc ggatcacgaa
      601 cagcgcggtc gcgaacttct cgccgagtga atcctcgagg cgcagatcga gatcatcttg
      661 cagccactgc gagaacggcg cgatgtcgtc gcggttcttc agcgtgacga tgatcgacga
      721 gtaggtcgtc gccgcttcct cgccttggta ctgacgattc cagcgcgaga tgtattggat
      781 cggcaccgtc atgccgatcg gcatcgcgcg attgctcatg ccaacgagca cggcctcgac
      841 gcggcgcggg cgatgttgga tcgccgtgtt gctgcccgcg accgtcgttt gcccgagacc
      901 gatcgagaac cgcatctggc cgagaccgcc gcgctgcgtg acccacttga cgaagtccgt
      961 ctcgatgatc ggcagaccgt gcgacttcgc gaactgaccg ttgtagagct cgatcatcgt
     1021 cggtgacatg acgatcggca cgcgatgctc gcaccgccgc gactcctcgt cgcagtagta
     1081 gcgttccggt tccgggcacg ggttcaggta ctcgcccttc ttgccgacga cccagaactc
     1141 gccggcgcgc caatcgaact gcgccggtgc tgcctggggg agaatcggga tagcagagcc
     1201 cgtcgcggga tccgcgggag gcggagccgc aggcggcgtc ggttgcggcg gaccttccgt
     1261 cggcggcgtt tggccgagct tggtctggcg tcctgcgggc ggcgccttcg gcgattgaat
     1321 cacttcgtct tcgtcggact tcggcggcgg catgcacgcg acgcgatgcg gatccttgtc
     1381 gtccgccatc cagtctttga acagctcgcg aatgcgttcg tcgtcttgca cgtacgacgc
     1441 gtcgacaccg tcggcaaagc cgccgacctc gaagcgaaga tcgctgcctt cgaagctgcc
     1501 gcggccaaac gccgggaacg tgagcgacat gcgcggcacg gcggccttca cctcggggcg
     1561 cgcgaggatc gtcttgacga tgttgtcgtc gagcttcttg ctgatgtcct tgccgagcag
     1621 cgacgcgcgc ggcgcgacga cctgcacttg ctcgagcggg aagatcttct cgaggaccgc
     1681 gccgatttgc tctgtcgacg cgaggaacac gacgaacgtc gcgatgccga tcacgatgcc
     1741 gaacgccgag aggaagaagt ggcgcgggct gcgcagcgtg ttcgccgcga ccattctcgt
     1801 gagcttgccg acgggcatca tgactagacg ggtcctgccg aagcggcgag cgcttcatcg
     1861 gtggtctcga cgagcatgcc gtcgcgcatc gcgatcacgc gcttggcaac gctcgaaatg
     1921 cggcgctcgt gcgtgacgat cagcagtgtg acgccatctt tcgcgttgag ctcgcggaag
     1981 aagtcgatca cttccctgcc cgtctcgctg tcgaggttgc cggtcggctc gtcgcacagc
     2041 agcaggctcg gtgagccaaa cagcgcccgc gcgatcgcaa cgcgctgctt ctgcccgccc
     2101 gacagctcgc ccggataccg gctcgcgaag tccgagagcc cgacgcggcg aagcgagtcc
     2161 ttcgcgcggc cctcgacgtc cttgctgttc ttcgagaacg ccgcaggcag cgtcacgttc
     2221 tgcagaaccg tcaggtgatc catcagattg aacgcctgga acacgaagcc catgctctcg
     2281 ttgcgcatct gcgcgagctt cgcgtcgctg gccttgcgcg tatcgacacc gaggacttcg
     2341 acctcgccgg catcgcacgt gtcgaggccg ccgatgatgt tgaggagcgt cgacttgcca
     2401 gagcccgatt gaccgacgag cgcgatcagc tcgccctttt cgatgtcgaa cgacacaccg
     2461 cggaggactt gcgtcttggc ggtgccttcg ccgtagctct tcgtgacgtc gcgcagtctg
     2521 acaaccggac tggccatcgc gcgaaaccta ccacacgctc tctagcgccg tgtgccgttc
     2581 gcctcgatca cgagcgacgt gccatcgaga cgtgcaccga tgtgcaggtt ttgccagttc
     2641 tgcaaccgct gcgcgagacg ctgcggtgaa ggcgcgccga tctgttcgaa caaccactgc
     2701 cacacgtcgg gcttgaggcc cgcgggaaga acgtcgaccg atgcgatcgg gggcggacca
     2761 ccttgcggcg cgccgctgcc gagcttcgcg agcaggccgt cgcccatcgc gccgatgaat
     2821 aaggtgttgg tgaggacgta atcgactgtc gcgacgaacg gcaccgagag gtgcttgccc
     2881 ttgtattggc cgtacgtgcg gtccttctcg aggcggctgc gcatcggcac ttgatcgagc
     2941 tgctcggcga tgaagcggct gtgcgtgaga tcgagcgaga ccgcgccgac gccgctcttg
     3001 tcgtcaggat cgaattgctg cacgagcccg cgcgcgctgc gtacgccgaa ctgatcgatc
     3061 atcgcggcga cgtcggatgc ctcgcgctgc atgcacggcg ccatccaccg ctgcatgaac
     3121 gccgcgtcga gattccactg cgccgagacc ggcatccctt gcgatgcgcc cgtccatccc
     3181 ggcggtggct gcagcaacgc gccgccgata ttctgcgcgc tgccgccgat gtcgaacgag
     3241 aggtgaccgt cgagcgtccg gccgtccgcc gtgaccgaca cgccgacgcg cgccaccgac
     3301 tcgaactgcc gcgcgcacgc cgctgcttcg ggcacgcgct tcgtgatcac cgcgaacgca
     3361 tcgcgcagat tcgcaaaccc agtcaggccc tgcccgctcg cctgcgcctt cgcccagtcc
     3421 cacgcctgtg tccacgtcgg tgcgccggcc ttgtggctgt gctcgaacca ggtgccgagc
     3481 tccgcgttca tgtccgcgaa gtgaacccac agccaatcct tgtcgatcgc ccagctcacc
     3541 tgaacggtgc cctcgatgcg cgccgtgaag acctcgacgt tgtcgacgat catcgacgtg
     3601 agcttcatgc cctgcgcgcg ctggccatcg agatatgccg ccatcgcctt cgggtctgcg
     3661 aggtggacga cgaacgtcgg atcgatgtcc tcggagaacg cgacgagcgc gccattcatg
     3721 tcgacgccga tctgcgcgac cgcatcggga ttgagcggat caatcccgag cagcacctgc
     3781 agcgcgcgcc cgatctcttc gacctcgccg ccgactgcca ttccgaagat gtccgccgcg
     3841 tcgtgaaacg cgagctgcgc cgaccgaaac gtgggtgcgg ccacgacgta tgtcggcctg
     3901 ggcggcacga accgcgcacc gacgaacacc tcggcgccgc ccccgcctcc gcctccactc
     3961 cccttttgac ttccaccaca tgccgcgact gccagcgcaa tcaggaagac gactcgcatt
     4021 gcgcgagact ctgccacgca accagccatc gtgccggtcg atagctcccc aaacggccgc
     4081 ccgtcctgtc cggccgagaa aggcgaaacc atgtattctg cgagtgtctc atgagcccga
     4141 tcggtggagc cggtggcccc ggcggcgtcg gtggcccgaa aggtccagac ggtcctgacg
     4201 atgtcgacga cgtggcagat gtcggcagcg agacgagcgt ctccagcacg accgccgcga
     4261 aagcagcggg tgggctcgag gcgatggcat cggagatcgc ggccggccgg ctgacgccac
     4321 gcgaagcggt cgacaagctc gtcgacgaga ttgcaggcac cgatcagctc ggggcgggag
     4381 agcgtgcgga gctgcgcgag ctgctcagcg atctcgtcga gaacgatccg tatttagcgt
     4441 cgctcgtcgg tcgcgcgtaa ctagtagacg acgtcgccga caaacagcgc gcccttcacg
     4501 ctgccgagcg gcccgacaaa ctgcaccggc tgcgtgccgt tcggcgggac ccagatcatc
     4561 gagaacgggc gcgtcatcgg cttgccgtgc tcgtcgaaga acgtcgcgac gaccatgatg
     4621 ttgccgccgc gcggcgc
//
LOCUS       DATLBZ010000362         5919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2658970, whole genome shotgun sequence.
ACCESSION   DATLBZ010000362 DATLBZ010000000
VERSION     DATLBZ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658970"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VMZ53_18580"
     CDS             complement(<1..705)
                     /locus_tag="VMZ53_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30526.1"
                     /translation="MRALVVLVLLAGTAHAQSTVTVTLNQNGQMLAADLGLSVQDLTA
                     TAQDRIDELYKVSRIDELLRAFANTAAFAQRGLTVDYDVDPGDVLVGVAGGGVHGDVA
                     IGTTNTLLGGSIINFGAMTGVNLARWSKPRWTVFGGGFYEQTTIHGLTGHLLTLGAHA
                     QFQAVPAHARGHAHWTGVAVTSGIEYARWSVGEASMSTIESHFTATGPSEHASIHMSS
                     TGQLDVLTTTYTIPIEV"
     gene            complement(720..2369)
                     /locus_tag="VMZ53_18585"
     CDS             complement(720..2369)
                     /locus_tag="VMZ53_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30527.1"
                     /translation="MATYRPGLWLLAAGLTGNVATWVSTTVAHQLMRTGELQRASMRY
                     ITIVGYAEIAMTLALVVGAVLCAIGCPKGERRALFVSIAALFAVYRILVMTPWLLSLG
                     GVDVSIDLHLTLMRGLPFLYIAAFAALAFIAKRGDGSKRLATYTLWLLAIRVGSYAMN
                     DVAAQGKWLPAIGRIVALGTLLCALAIVFDLLGAFASSGQRAPDARIRSNTRDAATWI
                     TGVLAVLGLGLAAVAVWGTKRTATIDDTKLTLIEGTIMFMSAAAALRALHRREIHLER
                     FGIATLVVIVAGRVVLGVYAGPVQQVELETHELPGLTISLPLGEKDVRYQFAELGNLS
                     ILTRHADLGLVDLSWSSDERDPLEDYRNATVRPDGTEIVPITDRARDVEITGVVWSSA
                     VAARLYASWKCPGDTRWYLLSLSRYSPGVDVIHQVASRIFATLRCESRAKLPAFAVAA
                     PEGFHAIWAAELTVGQVFQPAGLDPDNVEKTTIEATRLPEVAEESIRTIYEHADEHAY
                     RIAAVWRCYTLNVDVAIYSAAGPGASRESLLTAIRSAHCPR"
     gene            2442..2810
                     /locus_tag="VMZ53_18590"
     CDS             2442..2810
                     /locus_tag="VMZ53_18590"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS30528.1"
                     /translation="MTLHRIALAALLLIPMVANAEPRRVSKAVEFEYGKARIYSKQRA
                     ELQEIAKQCRANPAMTVTVEGHAGAYNEEDSIALGEHRADVVRFLLIKYGVNPANITA
                     LDNSRFGEPGRYVDLVLEVR"
     gene            2821..3267
                     /locus_tag="VMZ53_18595"
     CDS             2821..3267
                     /locus_tag="VMZ53_18595"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HUS30529.1"
                     /translation="MEGGFRDPPPAATLGPNKKHCYACANILDVRAELCPQCGVRQPV
                     VPHMAAPGFAPPPYGHPGYHAPPHAMVYANQAMVPYTSKSKVTAGLFALLLGGLGIHK
                     FYLGQVGSGIVYLLFCWTLIPGLIAFVEGIILLTMSDADFARRYPG"
     gene            3267..3860
                     /locus_tag="VMZ53_18600"
     CDS             3267..3860
                     /locus_tag="VMZ53_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30530.1"
                     /translation="MTVFLLSPARCSGERAEMLARSKRSELGAKLREGQAPLGDVFAW
                     LSALYFRGKLAYATRFGEALVMAPGEGLVSPAALISTKTLRAMGRVEIESPRFTKALT
                     QDALTLAERHSGNVVLLGSIATGKYVDALLRVFGDRLLFPATFVGRGDMSRGGLMLRA
                     AKSGEELEYCPVAGANRHGTRPSKLPKLPRERSIVAS"
     gene            3865..4440
                     /locus_tag="VMZ53_18605"
     CDS             3865..4440
                     /locus_tag="VMZ53_18605"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HUS30531.1"
                     /translation="MRRCWLLALVVAAGCDDGPVAQEGPIMVSPCDGMLTIERMTAQD
                     HVPPGSPIDFTNNPPDSGPHYPIWAAWDRQYTNLQRGYYVHNAEHGGVVLLYNCDPDC
                     PDVVASLVDVARNMAADSTCVAPITKRVIITNDPLLPPGVQVAAVAWNYAYTASCFDP
                     YVAKFAKDNYRHAPEDFCSEGASFGGMPITP"
     gene            4547..5413
                     /locus_tag="VMZ53_18610"
     CDS             4547..5413
                     /locus_tag="VMZ53_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30532.1"
                     /translation="MRGRTLYACALALCINCGPPSRDQGGGGGGDDDGTDPDGANADN
                     CAKGTELIYTIDQFNNRLSQFDPMTKTFTDLGSINCGSAGTPFSMSVDRMANAWVLYT
                     NGDMFWVSITGGLTCTKVQWTSPNGLKVFGMGFSTDMPGGSSEALYIGGGSTQVQSTY
                     TLARVDPVTMNAMPIGSQPALPEMTGNGNAELWGFMPEASTARVVQFDKTNGAILKTF
                     TEPMLAGTMSGYAFAHWGGDYWVFLQKGGETSTKVYQIDGMAGGIKTITPTTGRTIVG
                     AGVSTCAPVVIL"
     gene            complement(5422..>5919)
                     /locus_tag="VMZ53_18615"
     CDS             complement(5422..>5919)
                     /locus_tag="VMZ53_18615"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS30533.1"
                     /translation="GDPDLRVSALRALAGSGDKRGVEVMIGALRDGNAAVRETAIYGL
                     GSLRSDKARDALIDFSRTQDAGDRRSAVLALRDYSDPRAQQRIVELARDPDASVRNYA
                     MDYMPDTQAGAAVLKSILNDVSLDMNTRIRAAQSLRYRSYLDESTTAMIESVEADYYH
                     DYRDY"
BASE COUNT         1052 a         1948 c         1929 g          990 t
ORIGIN      
        1 gacctcgatc gggatcgtgt acgtcgtcgt gaggacatcg agctgccccg tcgagctcat
       61 atggatcgac gcgtgctcgc tcggtccggt cgccgtgaag tgactctcga tcgtgctcat
      121 gctcgcttca ccgacgctcc accgcgcgta ctcgatgccg ctcgtcaccg cgacgcccgt
      181 ccaatgcgcg tgaccgcgcg cgtgcgctgg cacggcttga aactgcgcgt gcgcaccgag
      241 cgtgagcaga tgacccgtga gcccgtggat cgtcgtctgc tcgtaaaagc caccgccgaa
      301 caccgtccac cgcggcttgc tccagcgcgc gaggttcacg cccgtcatcg cgccgaagtt
      361 gatgatcgag ccgccgagga gcgtgttcgt ggtgccgatc gcgacgtcgc cgtgcacgcc
      421 tccaccggcg acaccgacga ggacatcgcc cggatcgacg tcgtaatcga ccgtgagccc
      481 gcgctgcgca aacgcggctg tgttcgcgaa cgcgcgcagc agctcgtcga tgcgcgagac
      541 tttgtaaagc tcgtcaatgc ggtcctgcgc ggttgcagtc agatcctgca cgctgaggcc
      601 gagatccgcg gcgagcatct ggccgttctg attgagcgtg accgtgacgg tcgactgcgc
      661 gtgtgccgtt cctgccagca ggacgaggac gacgagcgcg cgcacgatca caccgtagct
      721 caccgaggac aatgcgcact gcggatcgcc gtgaggagcg attcgcgcga cgcgcccggc
      781 ccggccgccg agtagatcgc gacatcgacg ttcagcgtgt aacagcgcca caccgcggcg
      841 atgcgatacg cgtgctcgtc ggcatgctcg tagatcgtgc gtatcgactc ttcggccacc
      901 tctggcaacc tcgtcgcttc gatggtcgtc ttctccacgt tgtcgggatc gaggcccgca
      961 ggctggaaga cctgaccgac agttagctcc gcggcccaga tcgcgtgaaa gccctcgggc
     1021 gcggcgaccg caaacgcggg aagctttgcg cgcgattcgc agcgcaacgt cgcgaagatc
     1081 cgcgacgcga cctgatggat cacgtcgacg cctggcgaat atcgcgatag cgagagcaga
     1141 taccagcgcg tatcgccggg gcacttccag ctcgcgtaga gccgcgccgc gaccgcgctc
     1201 gaccacacga cgcccgtgat ctccacgtcg cgcgcgcggt ccgtgatcgg cacgatctcg
     1261 gtgccgtctg ggcgcacagt tgcgttgcgg tagtcttcga gcggatcgcg ctcgtcactc
     1321 gaccacgaaa gatcgacgag cccgaggtcc gcgtggcgcg tcaggatgct caggttgccg
     1381 agctcggcga actgatagcg gacgtctttc tcaccgagcg gaagcgagat cgtgagcccc
     1441 ggcagctcgt gcgtctcgag ctcgacctgc tgtaccggcc ccgcgtacac gccgagcacg
     1501 acgcgccccg cgacgatcac gacgagtgtc gcgatgccga agcgctcgag atggatctcg
     1561 cgccggtgaa gcgcgcgcag cgcggcggcc gcgctcatga acatgatcgt gccctcgatg
     1621 agcgtcagct tcgtatcgtc gatcgtcgcg gttcgcttcg ttccccacac cgccacagct
     1681 gcgaggccca gacccaacac ggcgagcacg cccgtgatcc acgttgctgc atcacgcgtg
     1741 ttgctgcgga tgcgcgcatc cggtgcacgt tgcccgctgc tcgcgaatgc accgagcagg
     1801 tcgaacacga tcgcaagcgc gcacagcaac gtaccgagcg cgacgatccg accgatggcc
     1861 ggcagccact tgccctgggc ggcgacatca ttcattgcgt acgatccgac acggatcgcc
     1921 aatagccaca gcgtgtacgt cgcgagccgc ttgctgccat cgccacgctt cgcgatgaac
     1981 gcgagagcgg cgaacgccgc gatgtacaga aacggcagcc cgcgcatcaa cgtgagatgc
     2041 aggtcgatcg agacatcgac gccgccgagc gacaagagcc acggcgtcat gaccaagatg
     2101 cgatagaccg cgaataaggc cgcgatcgag acgaacagcg cgcggcgctc tccctttgga
     2161 caaccaatcg cgcacagcac cgcaccaaca acgagcgcga gcgtcatcgc gatctccgcg
     2221 taacctacga tcgtgatgta gcgcatcgac gcgcgctgca gctcgccggt cctcatcagc
     2281 tggtgagcga cggtcgtcga gacccacgtc gcgacgttgc ccgtgagccc cgcggccaac
     2341 agccacaacc cgggccgata cgtcgccatc catcgaatct agacgaaggt aggtgcgcgg
     2401 aacacgtaaa caatgcgcag agcctgccga accgaggagg catgaccctc caccgcatcg
     2461 ccctcgccgc cctcctgctc atccccatgg tcgccaacgc cgagcctcgc cgcgtgtcga
     2521 aagcggtcga gttcgagtac ggcaaggccc gcatctactc caagcagcgg gccgagctgc
     2581 aggaaatcgc aaagcagtgc cgcgcgaacc cggccatgac cgtcacggtc gaaggtcatg
     2641 caggcgccta caacgaggaa gactcgatcg ccctcggcga gcaccgcgcg gacgtcgtcc
     2701 ggttccttct catcaagtac ggcgtcaacc ctgcgaacat tactgccctc gacaactctc
     2761 gtttcggcga gccgggccgc tacgtcgatc tcgtcctcga agtgcgatag cctcgaccga
     2821 atggaaggtg gctttcgcga tcctcccccg gcggcaacgc tagggccgaa caagaagcac
     2881 tgctacgcgt gcgcgaacat tctcgacgtc cgcgccgagc tgtgtccgca gtgcggagtc
     2941 cgccagcctg tggtgcccca catggcagcg cctggttttg cgccgccacc gtatgggcat
     3001 cccggttatc acgcgccgcc gcacgcgatg gtctatgcga accaagcgat ggtgccgtac
     3061 acgagcaaga gcaaagtgac ggcgggtttg ttcgcgctgc tcctcggcgg gctcggcatt
     3121 cacaagttct atttgggtca ggtcggatcg ggcattgtct atctgctctt ctgttggacg
     3181 ctgattccgg gtctcatcgc attcgtcgag ggcatcattt tgctcacgat gagcgacgct
     3241 gatttcgctc ggcgctatcc ggggtgatga ccgtattctt gctgagccct gcgcgctgca
     3301 gtggagagcg cgcggagatg ctcgcgcgat cgaagcgctc ggagctcggc gcgaagcttc
     3361 gcgaagggca agcgcccctc ggcgacgtgt ttgcgtggct gtcggcgctc tactttcgcg
     3421 gcaagctcgc gtacgcgact cgcttcggcg aagcgctcgt gatggcgccg ggcgaggggc
     3481 tcgtgtcacc tgcagcgctg atctcgacga agacgctgcg cgcgatgggc agagtcgaga
     3541 tcgaatcgcc gcgcttcacg aaggccctca cccaagacgc actcacgctc gccgagcgac
     3601 acagcggcaa tgtcgtgctg ctcggcagca tcgcgacggg caagtatgtc gatgcgctgc
     3661 tgcgcgtgtt cggcgaccgc ctcctgtttc ccgcgacgtt cgtggggcga ggcgacatga
     3721 gccgaggcgg cctgatgctg cgcgcggcga agagcggcga agagctcgaa tactgtcccg
     3781 tcgcgggtgc gaaccgtcac ggcacacgtc cgtcgaagct gcccaagctc ccgcgcgagc
     3841 gctcgatcgt ggcaagctga cgcgatgcgc aggtgctggc ttctcgctct cgtggttgca
     3901 gccggctgcg atgacggtcc tgtcgcgcag gagggcccca tcatggtgag cccgtgcgac
     3961 ggcatgttga cgatcgagcg catgacagcg caggaccacg tgccgcctgg ctcgccgatc
     4021 gacttcacga ataacccgcc ggacagcggg ccgcactatc cgatctgggc cgcgtgggat
     4081 cgccagtaca cgaatctgca gcgcggctac tacgtccaca atgccgagca cggcggcgtc
     4141 gtgctgctct acaactgcga tcccgattgc ccggatgtcg tcgcatcgct cgtcgacgtc
     4201 gcacgcaata tggctgcgga ttcgacctgc gtcgcgccga tcacgaagcg cgtgatcatc
     4261 acgaatgatc cgctgctgcc gccgggcgtg caggtcgcgg ccgttgcttg gaactacgcc
     4321 tacaccgcca gctgtttcga cccgtacgtc gcgaagttcg cgaaggacaa ctaccggcat
     4381 gcgccggagg acttctgtag cgaaggggcg agctttggcg gcatgcccat cacgccgtag
     4441 cgcgcgaatc gtgcgcctgc gtgcgcgtcg cgtgcagatg agctgacagc agctgtttcc
     4501 cgacggggag cgagcccgtc gcacgagagg actacgctcc tccgcgatga ggggacgcac
     4561 gctctacgca tgcgcgctgg cgctttgtat caactgtggg ccaccgtcgc gcgatcaagg
     4621 cggcggagga ggtggcgatg acgacgggac ggatcccgat ggcgcgaacg ccgacaactg
     4681 cgcgaagggg acggagctga tctacacgat cgatcagttc aacaatcgcc tgtcgcagtt
     4741 cgatccgatg acgaagacgt tcaccgatct aggatcgatc aactgtggct cggcgggcac
     4801 gccgttctcg atgagcgtcg atcgcatggc caatgcgtgg gtgctctaca cgaacggtga
     4861 catgttctgg gtctcgatca ccggcgggct cacgtgcacg aaggtgcaat ggacctcgcc
     4921 gaacgggctc aaagtcttcg gcatggggtt ctcgacggac atgccgggcg gctcgagcga
     4981 agcgctctac atcggtggcg gctcgacgca ggtgcagtcg acgtacacgc tcgcgcgcgt
     5041 cgatccggtg acgatgaacg cgatgccgat cggcagccag ccagcgcttc ccgagatgac
     5101 aggcaacggc aacgcggagc tctggggctt catgccggaa gcgtcgacgg cgcgcgtggt
     5161 gcagttcgac aagacgaacg gcgcgatcct gaagacgttc accgagccga tgctcgccgg
     5221 cacgatgtcg ggctacgcat tcgcgcactg gggcggcgac tactgggtgt tcctccagaa
     5281 gggcggcgag acctcgacga aggtctacca aatcgacggc atggcgggcg ggatcaagac
     5341 gatcacgccg acgacgggcc gcacgatcgt cggtgcaggc gtctcgacgt gcgcaccggt
     5401 cgtgatcctc tagcccgcga gctagtagtc ccgatagtcg tggtagtagt ccgcttcgac
     5461 ggactcgatc atcgcggtcg tgctctcgtc gaggtagctg cggtagcgca gtgactgcgc
     5521 cgcgcggatg cgcgtgttca tgtcgaggct cacgtcgttg aggatcgact tcagcacggc
     5581 ggcgcccgcc tgcgtgtcgg gcatgtagtc catcgcgtag ttgcgcaccg acgcatcggg
     5641 atcgcgggcg agctcgacga tgcgctgttg cgcgcgtgga tcgctgtaat cgcgaagagc
     5701 gaggacggcg ctgcgccgat cgccagcatc ctgcgtgcgc gagaagtcga tcagtgcatc
     5761 gcgggccttg tcgctgcgca gcgagccgag gccgtagatc gcggtctcgc gaaccgcggc
     5821 gttgccatcg cggagcgcgc cgatcatgac ctcgacaccg cgcttgtctc cggagcccgc
     5881 gagcgcacgc agcgcgctga cacgaaggtc gggatcgcc
//
LOCUS       DATLBZ010000363         6489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4130098, whole genome shotgun sequence.
ACCESSION   DATLBZ010000363 DATLBZ010000000
VERSION     DATLBZ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4130098"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VMZ53_18620"
     CDS             <1..227
                     /locus_tag="VMZ53_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30534.1"
                     /translation="LPSLDYKGKKVTKYLDDETSREALFDDGLVFRTDYPVDEKTIGD
                     KLKKAATIIEVMKRRKADEARAKGLHVANG"
     gene            complement(391..1236)
                     /gene="murI"
                     /locus_tag="VMZ53_18625"
     CDS             complement(391..1236)
                     /gene="murI"
                     /locus_tag="VMZ53_18625"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HUS30535.1"
                     /translation="MTMRHPTDGGTGSRRPIGVFDSGAGGLTVVRALRRALPGEDIIY
                     LGDTARVPYGSKSPRTVEKYSLACQRFLLERGVKLVLIACNTASANALPALREASPVP
                     VIGAVEPGAESALAATTNGHIGVIGTLGTVRSGAYAKAISERDPDAKLVALACPLFVP
                     LAEEGWTQGDDDVAMLVARRYLVQLFAQDPAIDTLVLGCTHYPLLRDVLTRVAAEVAG
                     RDVAVVDSASAMAEAARDALGSGPNKRTQAGRLEVFATDTSRLDELAPRFLGEPITGF
                     ELVDL"
     gene            1589..2737
                     /locus_tag="VMZ53_18630"
     CDS             1589..2737
                     /locus_tag="VMZ53_18630"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HUS30536.1"
                     /translation="MSEAAADRIRGLVEADPVVLVEIAAAIANEGRMPPDAITRVAQD
                     HAVRLPDADKARLRDAMEQMLMGRDVDAALEWLQQGGIVRVLFPELEATVDLVQETGR
                     QHKDVWAHTKQVVRQTVRRPLVRWAALLHDIGKVPTRTFTPEGVHFHGHAEVGARMFD
                     RVHSRFAFARDERQTIRFLVKHHLRTNQYSDQWTDSAVRRFHREMGPHMTDLLDLSRA
                     DITSKRPGRRKQLLEQISALADRVDVLVEADAKLPPLPGGVGNAIMDAFEIAPSRLIG
                     DLKRALESRIEAGELEARREDAYYVAYLARHDLVPNVAPEKREALIAAGGLIGDEARN
                     AEEDLEGPHKGVDRDDPSPGVLACGHDPDNDPCIHRDADPDDPELHRH"
     gene            2823..3215
                     /locus_tag="VMZ53_18635"
     CDS             2823..3215
                     /locus_tag="VMZ53_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30537.1"
                     /translation="MRPVVLACVLAGCGVSGPGDVAPPTAAEESAANQAAEDVSIESP
                     GDSITVDLDACANQRLFAWLPLGSMWILVHRRDGDHCELWLGGETEDPSYNGAASQYC
                     LFYRQGTLDVEVRNGGPARVDEPSCIDL"
     gene            complement(3216..4448)
                     /locus_tag="VMZ53_18640"
     CDS             complement(3216..4448)
                     /locus_tag="VMZ53_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30538.1"
                     /translation="MRWLLVTACVAGCYSPKVGDCVVACGKNDSCPPGLTCDNAVCRL
                     PEMVGTACSEPADASSTIDINPDDCTWALVSSNFAPADVPDTSPPAWDVTADETFTTG
                     GVDGHAVSQTGASPLWIVHKSRFSVAAGATLTIEGMHPLVIAADEIDIAGTIVTLNGS
                     GNATCDAEPGAPACNNASGGGGGGYGADGGDGGGPPADQGGAIAGITPSPLRGGCPGA
                     KGGNGQQNATDATPQLGGSPGAGGGALQLSARKTLHITGTIRANGFAGGPGRSDHAGG
                     NACPGFTGRGSGGGGGGGGGTLLLESCAIVIEPSAKLCASGGSGGGGAGGGNTDIGVA
                     GNDGSCDMQAIGGAGEAAGANGGNGGFDGLPATSGQQASRGGGGGGGAVGRIHIYQVS
                     GAPVTAGAVIRPPANRSQ"
     gene            4579..5997
                     /locus_tag="VMZ53_18645"
     CDS             4579..5997
                     /locus_tag="VMZ53_18645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30539.1"
                     /translation="MGTDPTISGDSLPRVQASEHSELTPNTMVGEYRIEGVLGQGGMG
                     RVYAAVHPVIGKRAAVKVLHPALSAEQESVDRFVQEARAVNQIGHPNIVDIFAFGTLP
                     DGRCYFVMEHLQGESLGKRIEHSPMPVVDALAILDAIAVALEAAHRKGIIHRDLKPDN
                     VFLVHVEGADSQVKLLDFGIAKLVGGDVPLQRTRTGNVMGTPAYISPEQARGQGVDHR
                     TDIYAFGAVAFEMLTGRWPFPATNAADMIAKHLFEEPPSAAQLNPDIDGEVDQLIKSM
                     LAKAADDRPTLEQARQQLRAFRNRILQRQALHEGGHTDPVVDISHVRLQTRDPLALSM
                     PIDPPAPSATVWEKRAGRERRLIWVGAAAIVIGATVAVMLVFGGTSSSSSTTPARTST
                     TPATTSTTPATTPATTTPTTPATTTRTTPAITTAPTTTASSKTTTTPATTTTTTPTKS
                     KTPKPRTKPTKASQPDDPDAPM"
     gene            6006..>6489
                     /locus_tag="VMZ53_18650"
     CDS             6006..>6489
                     /locus_tag="VMZ53_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30540.1"
                     /translation="MKRSVISCVSSVLGALLATSALAQPAPSPPTPLPTDPGEEAYQR
                     GRRHYDLHEWDAAVTEFKEAYRIRQDAASLFNIAQSYRLKGDCAQAASFYKTYRRNFS
                     AEKNIARVDQFITEMDECAKRTASTTSPTDSSSTGTTTGTTTGATTGATTGTTTGTTT
                     A"
BASE COUNT         1196 a         2277 c         2001 g         1015 t
ORIGIN      
        1 cgttgcctag cctcgactac aagggcaaga aggtgacgaa gtacctcgac gacgagacct
       61 cgcgtgaggc cttgttcgac gacggtctgg tgttccgcac cgattatccc gtcgacgaga
      121 agacgatcgg cgacaagctc aagaaggcag cgaccatcat cgaagtgatg aagcgtcgca
      181 aagccgacga agcgcgggcc aaaggcctgc acgtcgctaa cggctgatcg cgcagccctc
      241 gtacgcgtaa gcgagcaccc gcccctcggc gttcggcgtt cggcgttcgg ctgcgctctg
      301 ccctgacttg gcgagccact tcgtcactcg cgagactggc gaaatgcccc gaagcggggg
      361 cgtgcaccgc ttacgcttac gcttgcgggc tcacagatcg acgagctcga agccggtaat
      421 cggctcgccg aggaatcgcg gcgcaagctc gtcgaggcgc gacgtatcgg tcgcgaacac
      481 ctcgagcctt cccgcttgcg tgcgcttgtt cggcccactg ccgagcgcat cgcgcgcagc
      541 ttctgccatc gcgctcgccg aatcgacgac ggcaacatcg cgacccgcga cttctgcggc
      601 tacgcgcgtg agcacgtcgc gcagcagcgg gtagtgcgtg cagccgagca cgagcgtgtc
      661 gatcgctgga tcctgcgcga acagctgcac gagatagcga cgcgcgacga gcatcgcgac
      721 gtcgtcgtca ccctgcgtcc agccttcctc tgcgagcggc acgaacagcg ggcacgcgag
      781 cgcaacgagc ttcgcgtcgg gatcgcgctc gctgatcgct ttcgcgtatg cacccgagcg
      841 aaccgtgccg agcgtgccga tcacgccgat gtgaccgttc gtcgtcgctg ccagcgcgct
      901 ctctgcaccg ggctcgaccg cgccgatgac cggcaccggg ctcgcttcgc gcagcgcagg
      961 caatgcattc gccgatgccg tgttgcacgc gatcaagacg agcttcacac cgcgctcgag
     1021 caagaagcgc tgacacgcga gcgagtattt ctcgacggtg cgcgggctct tacttccata
     1081 aggaacgcgc gccgtgtctc ccaaatagat gatgtcttca ccgggaagcg cgcgccgaag
     1141 cgccctcacg acggtgagcc caccagctcc tgaatcgaac acaccaatcg gccggcgcga
     1201 tcccgtgcca ccgtcggtcg gatgacgcat cgtcatcgtc gtcgaatctt gccccataac
     1261 gtctccggag tgcgactcgc tgtcacagcc ggcaactcaa cttgccgtac tgatttgcgt
     1321 gtcgcaagag aaacgcctca gcgggcgcga cgcttccgcg tgctcttgca gcgggtccga
     1381 cgaccctcga atgcccacaa aagatcgagg aaggtcacag gcttcacggc gagatcttca
     1441 tagaagcgcg tgataccggg catcgaaagc tcggccgcgg gcaactgcgt ctcggtgatc
     1501 ctgccttgca ctgcgccgcg gcaacgagca cggtccgtac cagatccgca cgctccgatt
     1561 ggttggtgtg ctagttgtga cggcccccat gtctgaggcc gcagcagacc gcatccgcgg
     1621 cctcgtcgaa gccgacccgg tcgtgctcgt cgaaatcgcg gctgccatcg ccaacgaagg
     1681 ccggatgcct ccggatgcga tcacgcgtgt cgcgcaagat cacgctgtcc gcctgccgga
     1741 cgcggacaag gcgcgtctgc gcgacgcgat ggagcagatg ctcatgggcc gcgacgtcga
     1801 tgcagcgctc gagtggctgc agcaaggcgg catcgtgcgc gtgctcttcc ccgagctcga
     1861 ggcgaccgtc gacctcgtac aggagaccgg ccgccagcac aaagacgtct gggctcacac
     1921 gaagcaggtc gtccgccaaa cggtgcgccg cccgctcgtc cgctgggccg cgctccttca
     1981 cgacatcggc aaagtcccga cgcgcacgtt cacgcccgaa ggcgtccact tccacggcca
     2041 cgccgaagtc ggtgcgcgca tgttcgatcg cgttcactcg cgcttcgcat tcgcgcgcga
     2101 cgaacggcaa acgatccgct tcctcgtcaa gcatcacctg cgcacgaacc agtacagcga
     2161 tcagtggacg gactccgccg tgcgccgctt ccaccgcgag atggggccgc acatgacgga
     2221 cctcctcgac ctctcgcgcg cggatatcac gtcgaagcgc ccaggccgtc gcaagcagct
     2281 gctcgaacaa atctccgcgc tcgccgatcg cgtcgacgtc ctcgtcgaag ccgacgcgaa
     2341 gctgccgcca cttcccggcg gcgtcggcaa cgcgatcatg gacgcattcg agatcgcacc
     2401 gtcgcgcctg atcggcgacc tcaagcgcgc gctcgaatcc cgcatcgaag caggcgagct
     2461 cgaagcgcgt cgcgaagacg cgtactacgt cgcctacctc gcgcgtcacg acctcgtgcc
     2521 gaacgtcgcg cccgaaaagc gcgaagccct catcgccgcc ggcggcctga tcggcgacga
     2581 agcgcgcaac gccgaagaag atctcgaagg cccgcacaaa ggcgtcgacc gcgacgatcc
     2641 atcacctggc gtgctcgcat gtggccacga tccagacaac gacccgtgca tccaccgcga
     2701 cgccgatccg gacgaccccg agcttcaccg tcactagtct cagagatctg agaacgcgac
     2761 gcggcaagtc cgcagacgcg cagagcggca cggtcggcac gcaactcgaa taagccacgc
     2821 gcatgaggcc agtcgtgctc gcgtgtgtgc ttgcaggttg cggggtgagt ggaccaggcg
     2881 atgtcgcgcc accgacggca gccgaagaat cggcggcgaa ccaagcggcc gaggacgtgt
     2941 cgatcgaatc gccgggagac tcgatcacgg tcgatctaga tgcgtgcgcg aaccagcgcc
     3001 tgtttgcgtg gctgccgctc ggcagcatgt ggatcctcgt ccaccggcgc gatggcgacc
     3061 actgcgagct gtggctcggc ggtgagaccg aagacccgtc gtacaacggc gcggcgagcc
     3121 agtactgcct gttctatcgc cagggcactc tcgacgtcga ggtccgcaac ggtggccccg
     3181 cgcgcgtcga cgaaccgtcg tgcatcgacc tgtagctatt gcgagcgatt cgcgggcgga
     3241 cggatcacgg cgcctgcagt gaccggcgct ccgctcactt gatagatgtg gatgcgcccg
     3301 acagcaccgc cgcctcctcc gccgccgcgg gacgcttgct ggcccgacgt tgcgggcaga
     3361 ccgtcgaatc caccgttgcc gccgttcgcg cctgcggctt cgcctgcgcc gccgatcgct
     3421 tgcatatcgc acgagccatc attgcccgcg acgccgatgt ccgtgttgcc accacccgcg
     3481 ccaccgcccc cgctgccgcc gctcgcgcac agcttcgcgc tcggctcgat cacgatcgcg
     3541 cagctctcga gaagaagcgt gccgccgccg ccaccgccac caccgcccga gccgcggccg
     3601 gtgaatcccg ggcatgcgtt gccacccgcg tgatcactgc ggcccggccc gcctgcgaag
     3661 ccgttcgcgc ggatcgtgcc ggtgatgtgc aatgtcttgc gtgcagagag ctggagcgca
     3721 ccaccacctg caccggggct gccgccgagc tgcggcgtcg cgtcggtcgc attctgttgc
     3781 ccgttgccgc ctttggcgcc aggacaaccg ccgcgcaacg gagagggcgt gatgcccgcg
     3841 atggcaccgc cttggtccgc cggaggcccg ccgccatcac caccgtccgc gccgtagcca
     3901 ccgccgccgc caccactcgc gttgttgcat gcaggagcac ccggctctgc atcgcacgtc
     3961 gcattgccgg aaccgttgag cgtgacgatc gtacctgcaa tgtcgatttc gtcagcggcg
     4021 atcacgagag ggtgcatgcc ttcgatcgtc agcgtcgcgc ctgcggcaac gctgaaccgc
     4081 gacttgtgca cgatccatag cgggctcgcg cccgtctgac tcactgcgtg cccgtcgacg
     4141 ccgcccgtcg tgaacgtctc gtccgctgtg acgtcccacg cgggtggcga cgtatcgggg
     4201 acatctgcag gtgcgaaatt cgaagacacc agcgcccatg tgcagtcgtc gggattgata
     4261 tcgatggtcg agctcgcgtc ggcgggctcg gagcacgctg tcccgaccat ctccggcagt
     4321 cggcacaccg cgttatcgca agtgagtccc ggcgggcagg agtcgttctt gccacatgca
     4381 acgacgcagt cgcccacctt gggtgagtaa caaccggcca cgcacgcggt tacgagcagc
     4441 caccgcacga gctgcatcgt agcgcagtag cggcggcaaa aaccggtttc gctatgcctc
     4501 acgctcgcct tcggcgtgat ttcctcgtcg gggcggcagc tgtgtcatga tcggcgtgtg
     4561 ggtgagtcca accgaagcat ggggacagat ccgaccatct ccggcgactc gttgccgcgc
     4621 gtgcaagcgt ccgagcactc cgagctgaca ccgaacacga tggtcggcga gtaccgcatc
     4681 gagggcgttc ttggccaagg cggcatgggg cgcgtgtacg cagccgttca tccggtgatc
     4741 ggcaaacgcg ctgcggtcaa ggtgctgcac ccggcactct ccgccgaaca agagtcggtc
     4801 gatcgattcg tgcaagaggc gcgcgcggtc aatcagatcg gtcacccaaa catcgtcgac
     4861 atctttgcgt tcggcacgct gcccgatggt cgttgctact tcgtgatgga gcacctgcag
     4921 ggcgagagcc ttggcaaacg catcgagcac tcgccgatgc cggtcgtcga tgcgctcgcg
     4981 atcctcgatg cgatcgcggt cgcgctcgaa gcggcacatc gcaagggcat cattcatcgc
     5041 gatctcaaac cggacaacgt gttcctcgtt cacgtcgaag gcgcggactc gcaggtgaag
     5101 ctcctcgact tcggtatcgc gaagctcgtc ggtggcgacg tcccgctgca acgcacgcgc
     5161 accggcaacg tgatgggaac ccccgcgtat atctcgccgg agcaggcgcg cggccagggc
     5221 gtcgaccatc gaaccgacat ctatgcattc ggcgcggtcg cattcgagat gctcaccggc
     5281 cgctggccat tcccggcgac gaacgccgcg gacatgatcg cgaagcacct attcgaggag
     5341 ccgccgtccg ccgcgcagct gaaccctgac atcgacggtg aggtcgatca gctcatcaag
     5401 agcatgctcg cgaaagccgc cgatgaccgg ccgacgctcg agcaagcgcg ccaacaactc
     5461 cgcgcatttc gcaatcgcat cctccagcgc caagcgctgc acgaaggtgg gcacaccgat
     5521 cccgtcgtcg atatctcgca tgtccggttg cagacccgcg atccgctggc gctatcgatg
     5581 ccgatcgatc cgcccgcgcc gtctgccacc gtctgggaga agcgagcggg acgcgaacgt
     5641 cgcctgatct gggtcggcgc ggcggccatc gttatcggcg cgaccgtcgc agtcatgctc
     5701 gtcttcggcg gcacgtcttc ctcttcatcg acgacgcccg cgaggacatc gacgacgccc
     5761 gcgacgacat cgacgacacc cgcgaccacg cccgcgacca cgacgcccac gacgcccgcg
     5821 accacgacgc gcacgacgcc cgcgataaca accgcgccca ccaccaccgc gtcatcgaag
     5881 acgaccacga cgccggcgac gacaaccacc acaacaccga ccaagtcgaa aacgccgaag
     5941 cctcgtacga agccgacgaa ggcgtcgcaa cccgacgatc ccgacgcacc gatgtgaggt
     6001 cttccatgaa gcgctccgtc atctcgtgtg tctcgtccgt gctcggcgcg ctcctcgcga
     6061 cgtccgcact ggcgcaaccg gcaccctcac cgcctacacc gttgccgacc gatccgggcg
     6121 aggaggcgta tcaacgagga cgtcgtcact acgacctcca cgagtgggac gcggccgtta
     6181 ccgaattcaa ggaggcctat cggatccggc aggacgccgc atcgctcttc aacatcgcgc
     6241 agtcctatcg gctcaagggc gactgcgcgc aagcggccag cttctacaag acgtatcgac
     6301 gaaacttctc tgccgagaag aacattgctc gcgttgatca gttcatcacg gagatggacg
     6361 agtgcgcgaa gcggacagca tcgacgacat caccgacaga ctcatcatcg acgggaacaa
     6421 cgactggaac aacgactgga gcgacgactg gagcgacgac tggaacgacg actggaacga
     6481 cgactgcag
//
LOCUS       DATLBZ010000364         7306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1171890, whole genome shotgun sequence.
ACCESSION   DATLBZ010000364 DATLBZ010000000
VERSION     DATLBZ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171890"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1382
                     /locus_tag="VMZ53_18655"
     CDS             126..1382
                     /locus_tag="VMZ53_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30541.1"
                     /translation="MRWRLLAASIVLASVPARADNVDDKLAAITNDPAVKVDDPKSRE
                     LAAALMTEGVKQLQAQAYDQALANFLEAYAKFPSPKILLNIASTLAEMGRVADAANTY
                     QRYLSDPATGPERIAEVKQVLVDLDAKLTLLTVRVQPKGSEVSIDAGPFVAVGSALVT
                     RVRPGIHLVRVRNRLATGEVTVNGFEGEAKDIALSAIATVSQGDTTTPTEPPPPPPPA
                     AEQVNGWLVDGTRYGTADATSNERHVRATFSGPEVKAVVPALALETEDQEFAVQPVVV
                     ERRISSGVAALLRIDGKRRGVAGGVGFALAPSDAIEVELDGLKSSIWGLYAGVRLRFL
                     TGWVRPYVGGGVPVFFFENEQDMQNTAVFGVRGAAGAEGRINGHLSVQFDFGFEHFFN
                     VEDKLLAGKRPEADVFVPTVGVIGRL"
     gene            1382..3937
                     /locus_tag="VMZ53_18660"
     CDS             1382..3937
                     /locus_tag="VMZ53_18660"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HUS30542.1"
                     /translation="MRKLVAIAIVVCVAVVAHAVGNGDIRAVTPVTVVTVTSTTTGGN
                     GNATLQNTTSATTYNVLIGSDATCDPAVSFSIAGGNPIVGFSPQTSRTVQIACPSRGS
                     DAMRRCLFHATNSANATPLADFFGLCLYGSTPGTLVPQSTSLDFGTVTVGQEQTRTLT
                     ILHNGAGAQHIRRIYLQTGDVDGNFRFSAPCNPDAAYCDENLNAQVDVNGSIAVLVKC
                     TPQKAGSHTSQLYVGTDTFQLLSVPVTLTCTGSAATAPVLGVNPSRVELVKPTEVVGD
                     NEKTTVHLANPGGATLVITDVRTVDVDTGAAVDWTYTASGACSGQITTMCVLDPGETI
                     DLDLTFDPSAIGRRRATLLISYKDTVDRTTEIPLDGTGLGATLRVVGAASSLSFGSVP
                     IGRQSLLQIELANTGNRDTTAMVSVGPSSTPPFTVSPGPTTLVTPTAHRTLTVTCAPT
                     MAGSASTTVTAQSNDAFQSPPITIAASCEGSTLDLFSSPTSLMLGEVRNGGAPLTRSV
                     MLLSDSGAPITLAGPPRLETANSNITIHPPASMTTPTSFDVDINPPATQGQLSATILV
                     EATNGETLRIPLAGRIVKASYIVASAVDLGTFCIGQPTTSSNISLVSDGTATLQLTAP
                     TLSQATSPFELSVTSPSMYPSSLPSGAAATLAITPHRQTAVTTVTDTLTWHTDVVGAL
                     TATTTLSARFTDRGGAIAPPALDFGKVTVHVFSDDGQRVVLQNCNDTPLMLDPPMIRT
                     PFSIDSPNFPAVLDPNETIAFSVGFHPTRKGVVSDTLRISSPQLPDGPLEVMLVGEGE
                     APEPMTPDGGMGSNGNGDTSFYACACSSGSSSPAGGLAVLVALLAVVVPWRRR"
     gene            3922..6741
                     /locus_tag="VMZ53_18665"
     CDS             3922..6741
                     /locus_tag="VMZ53_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HUS30543.1"
                     /translation="MEATMKTPRHRRSFAAVCVIATVAGTAYAQLSVTPQTASTNMGT
                     IPQNTPGRVVFSIRNTSTSSVAITGFAPQGGAGCTGVTINPSTATLMPNQQQQFVATT
                     SGFNFSPAVCTWNISSTPSSGATVSTSFSTALVASPADVQPTQMDFGSQFAAGESQDV
                     TVSSYGTGGSGYAMISNDPANKFRFDESVCGTGNFHNCMIAIPTSGNTVSLAVNCNPQ
                     MITGTPANVSIYGGSGFGSGFGSQLLGQVALSNCIAGNAIQFENAPMTIVTNPNTTGS
                     GSAHIISLSGMGNLDSASLVTGDSNFKINDCGLQTCTFTNRSLDTFLSIECVGTTAGT
                     TAQLMVYGSGVGSDSGTITCVSTGSTGILTALPNPLPFGQVDVNPNPAPIDHFTLYNM
                     GGSTVSGVTVSPPMNTTDWSVSGCSSLSPCSIGAGSSQDIDVTFHPTTHGDKTTNVTV
                     SATGPGIPFMVSLTGEGMGGVMSVAQPQGPPYVLDFGTIPRGQPFTRTLSLSNIGNKT
                     FTANVSTPSSPYAITPMASQLITPLAQKDYTVTCESPTASASNDQTLSITSTAYANGS
                     ASVSLKCAIADTVVQVSPQQFDFGEVRTGTARRELSVTVTNPAGAAAAAHVTSMQLHE
                     PRTGLSLQPAMTDKVLQPGESVSATLVLTTEEDSDLSGELLDITVDSAMLSLPVTGKV
                     VTARSSLTPDAIDLGTACVGSLVSADVTLRNEGTATLQVERPMMDATFVASVSGAMTF
                     PTPLPPAQELHAVVEPAVTTVGSASGTLTWDDDVPNHYMVPAKVEYITTGTALSPALL
                     DFGSIAVDAAGFPKRVTVENCDLEPTDITIKAITTQNSPVGAWQLEPRLGTKKTLPPH
                     DKQAITVTFRPPARGRYEADLEVETSFGPRRIHLRGDAVGRDFDNTSFYACSCSSLSP
                     GKSWPIFAAVLFIIFRRRRGSSSAR"
     gene            complement(6701..>7306)
                     /locus_tag="VMZ53_18670"
     CDS             complement(6701..>7306)
                     /locus_tag="VMZ53_18670"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HUS30544.1"
                     /translation="ILDCYQSIGAVPIDVVDLGVSFATGGSVKYVCGGPGAAWLYVRK
                     DLIEQFAPRVTGWFGNEAPFAFTMPEQTYADTVWRYMGGTPAVAALYQSRAGQSLIAE
                     IGVRKIREKSLVMTQKCIDWVDELGMTLNSPRPAETRGGSVVFDFVGSADVCRELNRR
                     KFFCDHRPNAGIRIAPHYYSKPEEVDLFFNELKKIRGGAGK"
BASE COUNT         1308 a         2532 c         2272 g         1194 t
ORIGIN      
        1 gcgccgccgg ttgtgacgaa ggtcaagcct ccgaaaaccc cgaagccgcc gatcgcgacg
       61 ccaccagaca atcgccacac caagaacggc tttccgacgg aggtttcgcc gtaaactaga
      121 gcggcatgag gtggcggctc ctcgcagcga gcatcgtgct ggcaagcgtg cccgcccgcg
      181 ccgacaacgt cgacgacaag ctcgccgcga tcacgaacga tccggcggtg aaggtcgacg
      241 atccaaaatc acgcgagctc gcagccgcgc tcatgaccga gggcgtcaag cagctgcaag
      301 cgcaggcgta cgaccaggcg ctcgcgaact ttctcgaggc gtacgcgaag ttcccgagcc
      361 ccaagatcct cctcaacatt gcatcgacgc tcgccgagat gggacgcgtc gccgatgccg
      421 cgaacacgta tcagcgctac ctcagcgatc ccgcgacggg ccccgagcgc atcgctgaag
      481 tgaagcaggt cctcgtcgat ctcgacgcga agctgacgct gctcaccgtc cgcgttcagc
      541 cgaagggctc cgaggtctcg atcgacgccg gtccgtttgt cgccgtcggc agcgcgctcg
      601 tcacgcgcgt gcgccccggc atccacctcg tgcgcgtgcg caaccgcctc gcgaccggcg
      661 aggtcaccgt caacggcttt gaaggcgaag cgaaggacat cgctctgtcc gcgatcgcga
      721 cggtgagcca aggcgatacc acgacgccga cggagccgcc gcctcctcca cctcccgcgg
      781 ccgaacaagt caacggctgg ctcgtcgacg gcacgcgcta cggaaccgcc gacgcgacga
      841 gcaacgaacg ccacgtgcgc gcgacattct cgggaccgga agtcaaagcc gtcgtgccgg
      901 cgctcgcgct cgagaccgag gatcaagaat tcgccgtgca gccggtcgtc gtcgagcgtc
      961 gcatcagctc cggcgtcgcg gcgctgcttc gcatcgacgg caagcgccgt ggtgtcgcgg
     1021 gtggcgtcgg cttcgcgctc gcgccatcgg atgcgatcga ggtcgagctc gatggcttga
     1081 agtcgagcat ctggggcctc tacgccggcg ttcggctgcg attcctcacc ggttgggtgc
     1141 ggccgtatgt cggtggcggc gttcccgtat tcttcttcga gaacgagcaa gacatgcaga
     1201 acaccgcggt gttcggtgtg cgcggcgctg ccggtgccga gggccgcatc aacggccact
     1261 tgagcgtgca gttcgacttc ggcttcgagc acttcttcaa cgtcgaggac aagctgctcg
     1321 caggcaagcg gcccgaagcg gacgtgttcg ttcccaccgt tggcgtgatc ggccgcctgt
     1381 gatgcgcaag ctcgtcgcga tcgcgatcgt cgtctgtgtc gctgtggtgg cgcacgcggt
     1441 cggcaatggt gacattcgcg ccgtcacgcc ggtcacggtc gtcacggtaa cgagcaccac
     1501 gacgggcggc aacggcaacg cgacgctgca gaacacgacg agcgcaacga cgtacaacgt
     1561 gctgatcggt tccgatgcga cgtgcgaccc ggcggtctcg ttctcgatcg caggcggcaa
     1621 tccaatcgtt ggcttctcgc cgcagacctc gcgaaccgtg cagatcgcgt gtccatcgcg
     1681 cggctccgat gcgatgcgcc gctgcttgtt ccacgcgacg aacagcgcga acgcgacgcc
     1741 gctcgcagat ttcttcggcc tctgtctcta tggctcgacg cccggcacgc tcgtcccgca
     1801 gtcgacgtcg ctcgacttcg gcacggtcac cgtcggccaa gagcagactc gcacgctgac
     1861 gatcctgcac aacggcgcag gggcgcagca cattcgccgc atttatttac agaccggcga
     1921 cgtcgacggg aacttccggt tctctgcacc gtgcaacccc gacgctgcgt actgcgacga
     1981 gaacttgaat gcacaggtgg acgtaaacgg atcgatcgcg gtgctcgtca aatgcacgcc
     2041 gcagaaagcc ggctcgcaca cgtcgcagct ctacgtcggt accgacacgt tccagctgct
     2101 ctcggtgcca gtcacgctga cgtgcaccgg cagcgcggcg actgcaccgg tgctcggcgt
     2161 gaatccctcg cgtgtcgagc tggtcaagcc gaccgaggtc gtcggcgata acgaaaagac
     2221 caccgtgcat ctcgcgaatc ccggcggtgc gacgctcgtg atcactgacg tgcgcaccgt
     2281 cgacgtcgac acgggcgctg ccgtcgattg gacgtatacg gcgtccggcg cgtgctccgg
     2341 ccagatcacg acaatgtgcg tgctcgatcc gggcgagacg atcgatctcg atctgacgtt
     2401 tgacccgagc gcgatcgggc ggcgccgcgc gacactgctc atctcgtaca aggacaccgt
     2461 cgatcgcacg accgagatcc cactcgacgg aacggggctc ggcgccacgc tgcgcgtcgt
     2521 cggcgctgca tcgtcgctgt cgttcggctc cgttccgatc ggccggcagt cgctgctcca
     2581 aatcgagctc gcgaacaccg gcaatcgcga caccaccgcg atggtttccg tcgggccgag
     2641 cagcacgccg ccgttcaccg tgtcgccggg accgacgacg ctcgtgacgc cgaccgcaca
     2701 caggacactg accgtcacgt gcgcaccgac gatggcgggc tcggcgagca cgaccgtcac
     2761 ggcgcagtcg aatgacgcgt ttcagagccc accgatcacg atcgccgcgt cgtgcgaagg
     2821 cagcacgctg gatctgttct cgagcccgac gtcgctgatg ctcggcgaag ttcgaaatgg
     2881 tggagctccg ctgacgcgct cggtgatgct cctgtccgat tcgggggcgc cgatcacgct
     2941 cgcaggtccg ccgcggctcg agacggcgaa tagcaacatc acgattcacc caccggcgag
     3001 catgacgacg ccgacgagct tcgacgtcga catcaatccg ccggcgacgc agggtcagct
     3061 gtcggcgacg attctcgtcg aggcgacgaa cggcgaaacg cttcgcatcc cgctggcggg
     3121 ccgcatcgtg aaggcgtcgt acatcgtcgc gagcgccgtc gatctgggca cgttctgcat
     3181 tggccagccg acgacgtcgt ccaatatctc gctggtctcc gacggcaccg cgacgctcca
     3241 gctcaccgca ccgacgctct cgcaagcgac gtcgccgttc gagctatctg tcacgtctcc
     3301 atcgatgtat ccgagctcgc tgccctcggg cgccgcggcg acactcgcga tcacgccgca
     3361 tcgccagacc gcggtgacga cggtgacgga cacgctcacc tggcacaccg atgtggtcgg
     3421 cgcgctgacc gcaacgacga cgctcagcgc tcgcttcacc gatcgcggcg gtgcgatcgc
     3481 gcctcctgct ctcgacttcg gaaaggtcac cgtccacgtg ttctccgacg acggccagcg
     3541 cgtggtgctg cagaactgca acgacacgcc cctgatgctc gacccgccga tgatccgcac
     3601 gccgttctcg atcgacagcc cgaacttccc cgccgtcctc gatccaaacg agacgatcgc
     3661 gttctcggtc ggtttccacc cgacgcgcaa aggcgtcgtc agcgacacgc tgcgcatcag
     3721 ctcgccgcaa ctgcccgatg gaccgctcga ggtgatgctc gtcggcgaag gcgaagctcc
     3781 cgagccgatg actcccgacg gcggaatggg ctcgaacggc aacggggata cgagcttcta
     3841 cgcgtgcgcc tgctcgtcgg gctcgtcgag tcccgcgggc gggcttgccg tcttggttgc
     3901 gctactcgct gtcgtggtcc catggaggcg acgatgaaga cgccgcgcca tcgccgatcg
     3961 ttcgccgccg tttgcgtgat cgcgacggtc gcgggtaccg cgtacgcgca gctctccgtg
     4021 acgccgcaga ccgcctcgac caacatgggg acgatcccgc agaacacacc gggccgcgtc
     4081 gtgttctcga tccgcaacac gtcgaccagc tcggtcgcga tcaccggttt cgccccgcaa
     4141 ggcggcgccg gctgcacggg cgtcacgatc aatccatcga cggcgacgct gatgccgaac
     4201 cagcagcagc agttcgtcgc gacgacgagc ggcttcaact tcagtcccgc cgtctgcacg
     4261 tggaatatct cgtcgacgcc atcgagcggc gccaccgttt cgacgtcgtt ctcgacggca
     4321 ctcgtcgcat ctccggccga cgttcagccg acgcagatgg acttcgggtc gcagttcgcg
     4381 gccggcgagt cgcaagacgt cacggtgtct agctacggaa caggcggctc gggttacgcg
     4441 atgatctcga acgatccggc gaacaagttc aggttcgacg agagcgtgtg cggcaccggc
     4501 aacttccaca actgcatgat cgcgatcccg acgtcgggca atacggtttc gctcgccgtg
     4561 aattgcaacc cgcagatgat cacgggcacg ccggcgaacg tctcgatcta tggcggctcg
     4621 ggattcgggt cgggattcgg ctcgcagctg ctcggtcagg tcgcgctgtc gaactgcatc
     4681 gctggcaacg ccatccaatt cgaaaacgcg ccgatgacga tcgtcacgaa tcccaacacg
     4741 accggcagcg ggtccgcaca catcatctcg ctctcgggaa tgggcaacct cgacagcgcg
     4801 tcgctcgtga ctggcgatag caacttcaag atcaacgatt gcggccttca gacgtgcacg
     4861 ttcacgaatc gctcgctcga cacgtttctc tcgatcgagt gcgtcggcac gacggccggt
     4921 acgaccgcgc agctcatggt gtacggctca ggcgtcggca gcgactccgg cacgatcacc
     4981 tgcgtctcga ccggctcgac gggaatcttg acggcgctac cgaatccgct gccgttcggc
     5041 caagtcgacg tgaatccaaa cccggcgccg atcgatcact tcacgctcta caacatgggc
     5101 ggttcgacgg tgagcggcgt gaccgtctcc ccacccatga acaccaccga ttggagcgtg
     5161 tccggctgct cgtcgctgtc gccttgctcg atcggcgcag gctcgtcgca ggacatcgac
     5221 gtgacgttcc acccgacgac acacggcgac aagacgacga acgttacggt cagcgcgacg
     5281 ggacctggca ttccgttcat ggtcagcctc accggcgaag gcatgggcgg cgtcatgtcc
     5341 gtcgcgcagc cgcagggccc gccgtacgtg ctcgacttcg gcacgattcc tcgggggcag
     5401 ccgttcacgc gcacgctcag cctctcgaac atcggcaaca agacgttcac cgcaaacgtg
     5461 agcactccgt catcgccgta cgcgatcacg ccgatggcgt cgcagctcat cacgccgctc
     5521 gcgcagaagg actacaccgt cacgtgcgag tccccgacgg cgagcgcctc caacgatcag
     5581 acgctgtcga tcacgtcgac cgcgtatgca aacgggagcg caagcgtgag cctcaagtgc
     5641 gcgatcgccg acaccgtcgt tcaggtgagc ccgcagcagt tcgacttcgg cgaggtccgc
     5701 accggcactg cgcgacgcga gctgtccgtc accgtcacga atccagccgg cgcggctgcg
     5761 gctgctcatg tcaccagcat gcagctgcac gagccacgca ccgggctttc gcttcagccc
     5821 gcgatgaccg acaaggtgct gcagcccggc gagagcgtga gcgcgacgct cgtgctcacg
     5881 accgaagaag acagcgacct cagcggtgag cttctcgaca tcaccgtcga tagcgcgatg
     5941 ctctcgctgc cggtaaccgg caaggtcgtc actgcgcgct cgagcctcac gcccgacgcg
     6001 atcgatctcg gcaccgcgtg tgtcggcagt ctcgtcagcg cggacgtgac gttgcgcaac
     6061 gaaggcactg cgacgctgca ggtcgagcgg ccgatgatgg acgcgacgtt cgttgcatcg
     6121 gtatccggcg cgatgacgtt cccgacgccg ctgccccctg cccaagagct gcacgcggtc
     6181 gtcgagccgg cggtcacgac ggtaggctcg gcgagcggaa cgctgacgtg ggatgacgac
     6241 gtgccgaacc actacatggt gccggccaag gtcgagtaca tcaccaccgg aacggcgctc
     6301 tcgcccgcgc tcctcgattt cggcagcatc gccgtcgacg ctgcaggctt cccgaagcgc
     6361 gtcaccgtcg agaactgcga tctcgagccc acggacatca cgatcaaagc gatcacgacg
     6421 cagaattcgc cggtcggcgc atggcagctc gagcctcgcc tcggcacgaa gaagacgctg
     6481 ccgccgcacg acaagcaggc gatcacggtg acgttccgcc cacccgcacg tggtcgctat
     6541 gaggcggacc tcgaagtcga gaccagcttc ggcccgcggc gcatccatct ccgcggcgat
     6601 gcagtgggcc gcgacttcga caacacgagc ttctacgcgt gctcttgcag cagcctgtca
     6661 ccggggaaga gctggccgat cttcgcggcc gtgctgttca ttattttccg gcgccgccgc
     6721 ggatcttctt cagctcgttg aagaagaggt cgacctcttc cggcttcgag tagtagtgcg
     6781 gcgcgatgcg aatgccggcg ttcggccggt gatcgcagaa gaacttgcga cgattgagct
     6841 cgcgacacac atcggccgag ccgacgaaat cgaagacgac ggagccaccg cgcgtctccg
     6901 ccggacgtgg gctgttgagc gtcatgccga gctcgtcgac ccagtcgatg cacttctgcg
     6961 tcatcacgag gctcttctcg cgaatcttgc gcaccccgat ctcagcgatg agcgactgcc
     7021 ccgcgcgtga ttgatagagc gccgcgaccg caggcgtgcc gcccatgtag cgccacaccg
     7081 tgtcggcgta cgtctgctcc ggcatcgtaa acgcgaacgg cgcttcgttg ccgaaccacc
     7141 cggtcacgcg cggcgcgaac tgctcgatca gatccttgcg gacgtatagc cacgcggcac
     7201 ctggaccgcc gcacacgtac ttcaccgagc cgcccgtcgc gaacgacacg ccgagatcga
     7261 cgacatcgat cggcacggct ccgatcgact gatagcaatc gaggat
//
LOCUS       DATLBZ010000365         7357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3206617, whole genome shotgun sequence.
ACCESSION   DATLBZ010000365 DATLBZ010000000
VERSION     DATLBZ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206617"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VMZ53_18675"
     CDS             complement(<1..603)
                     /locus_tag="VMZ53_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30545.1"
                     /translation="MTTPWARPTYEALRAKLASREGERAGITLERAKLDAWLAAAAPP
                     DVPYDAVVLSADALRTRYPDSPLERRSLHMHGKLRMTHRALPSQSVLVLALFSDVAAD
                     YFVESMAADVAHHVNDHYGRDLAYADAQANIPIILKEVATAAGLGHIGKNALFFSKRF
                     GFNCKLEVILLDAPVDRYDAVPSANGSLEADDWKLADCASC"
     gene            complement(711..1343)
                     /locus_tag="VMZ53_18680"
     CDS             complement(711..1343)
                     /locus_tag="VMZ53_18680"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS30546.1"
                     /translation="MTVTAWLDRVLYRAPFEGSSARRYADLARPAFGDLDDRLLAGWP
                     ALTQAKCLLDLGFGPGAFARRALARFPALQVIAVDPSRDFARPHPGLHVVRASGEALP
                     LATDSCDAAMCLSSLRHVRDRAATLRELRRVVAGSLMIVELDPAASARRIAAHADGIA
                     SPLLRRAFGPLVVRTAPSLSSIEALARNAGWRIHARRDDEQQPVYIMELR"
     gene            1410..3338
                     /locus_tag="VMZ53_18685"
     CDS             1410..3338
                     /locus_tag="VMZ53_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30547.1"
                     /translation="MRGPRCFVVVLAFAAGCGDSIDVPDRCNPLGGQGCMLPWPSSAY
                     EVDDPSTVTGRRVEVPIESMPKNGDDISVDPVWLNRWDGFSPSGPMLAAFASGVSPEG
                     LPSFKNPDESLAADSPIIVVDMATGERAPFFAEVDQNTKDVLKRNLIIRPLARLHTNA
                     HYAVGITKKVKAADGGELSISEGFAAALSGDGDHPRFHPSAYKDIFAALEQAGVAKTD
                     LALAWDFHTASDDFIRRDLTTMRDAAITAMGTNGANLSFTVTNQPAATGIYKAYTGTF
                     KSPAFLTNGEADESVMRRDAKGIPQMQGMWDAPFAALVPDCVKTMPLPRPTIVFGHGL
                     FGSASEYLKDNFTIELAQSNCFIIVAGDFIGFTSRQFALAALAVNDMNRGTQVTEKLT
                     QSLIDFITLETLTRNQLGQHAEFQYNGQSVIDPAQTYYVGGSLGGIMGNTFMAYDPNI
                     KRGVLAVPGGVWSMLIERSFAWHALIGASQGSYRDAEVYELNLALFGMAFEPIDPIST
                     AAHVIKDPLPGVPAKNILIWYTLGDSLVSNITTEMIAREMGMDLLSPEVKPVWRMPGK
                     AGPLVNGVVNYNEHPTPLPPETNIPPSADNGTHSGVNRNQSALRQVYEFLLQGQQVVQ
                     TCGGTTPAPCDCTTGACD"
     gene            3380..5356
                     /locus_tag="VMZ53_18690"
     CDS             3380..5356
                     /locus_tag="VMZ53_18690"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HUS30548.1"
                     /translation="MQGICRALVIGVVVAACGNPSRNDNDNVRPDALNQCTMDGAHRC
                     NGSSYETCTGGQWMSALDCPAFCDEVRGCVECQAGQTYCKGGNVWRCADDGTGGMQTQ
                     ECTGINVCDAGSCVDACATAAVNKSYIGCDYFAVDLDNALETNGTSNGSCPAGQTSEM
                     LNVCFTQMGGGTVLGQCDPQDDGTAPHTCPAGTTCQSKLVCATDAQHGAFAVVISNPQ
                     AKDAHVTITGPQGTQIMKTVTAGQVLPIPMQQGTGIPDQSVEGTGKFRKAYRIQSDIP
                     IVAYQFNPLDNSSAVFSNDASLLIPSATYDTDYYVLTEPTLDRRNSSGGFKDPYYGFM
                     TVVAWQDGTQIAVTPTAAIMPSQTQSAIAAGTTATFTLNAFEVLQLEASGSGDLTGTH
                     ITSPNMMSFGVFAGHEATTLDDPRVTSPNPSKYPAGPGYADHLEEMLFPSSTWGKSFA
                     IARTKPRINETDILRIVAQMPGTNVSFTPPLAAPGAPSATPGPVVGTCPALDAGKFCD
                     VFIAGDVEITADKPILVGHMMESDSWIGNSGLIPGATGDPSLAIATPTEQFRKEYTIL
                     VPAAYAENYVSIAAAATGGVTVERILPNPMSIPVTLATFPGGGTHRAARVPLSAGQYK
                     ITCADGCGIAVYGYSPQVSYMFAGGLDLKPIVIF"
     gene            5437..7356
                     /locus_tag="VMZ53_18695"
     CDS             5437..7356
                     /locus_tag="VMZ53_18695"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HUS30549.1"
                     /translation="MKCILVAALAACGPSSNHGDDGVDAAVDCKPEGAHQCLGSTYQT
                     CTGGTWQTALDCQSACIEDLGCVDCHPGEQFCKDGDVWTCGPDGMPGSVTQACTGATT
                     CAGATCVDACAEAAASKSYIGCEYWAVDLDNALEVLGGPPPFGAPCNDTTFGYGTGVA
                     ATRDVCFKSQGNTTTYAGLCDPPNNACPTGYTCQSKSVCILNAQQSPFAIVVSNPQPK
                     DASVTVTGPAGQTVTKVIPAGQVVPILPQAGNAIPDASIDGTIKTRGAYKVVADRPIV
                     AYQFNPLDNVDVFSNDASLLIPRTAFDTDYYAMSYPTLDRRTVTGSTIPQHNYYGYLT
                     VVAWQDNTQIEVTPTTAIQASATQTTIAAGVTTAFTLNAFEVLQLEAAPPAGDLTGTH
                     IASTNMMSFGVFAGHEATNFGETTAPDSTHTSGPCCADHLEEMMFPSSTWGKEFAISR
                     SQQRTNEPDMLRILAQKPNTTLTFKPAPASGTCGTLNAGQFCQVKISGDTEIVATEPI
                     LVGHYLEASMWRDNPLFGIPTWVGEGDPSMAIAVPTEQYRKDYAILVPSQYSKSFMSV
                     AAAATGGVFVDGTAVTLAAFPGGGTHRAARVMLTPGAHKVTCPDGCGITVYGYSDAVS
                     YMFAGGLDLKPIVIF"
BASE COUNT         1308 a         2430 c         2449 g         1170 t
ORIGIN      
        1 gcacgacgcg cagtcggcga gcttccagtc gtcggcctcg agggagccgt tcgcagacgg
       61 gacggcgtcg tagcgatcga ctggtgcgtc gagcaggatg acttcgagct tgcagttgaa
      121 gccgaacctc ttcgagaaga agagcgcgtt cttgccgatg tgaccgaggc ctgcggccgt
      181 tgcgacctct ttgagaatga tcgggatgtt cgcttgcgcg tcggcgtacg cgaggtcgcg
      241 gccgtagtgg tcgttgacgt ggtgcgcgac gtcggccgcc atcgactcga cgaagtagtc
      301 ggcggcgacg tcggagaaca gcgcgaggac gaggaccgac tgagagggga gcgcgcgatg
      361 tgtcatgcgc agcttgccgt gcatgtggag actgcggcgt tcgagcgggc tgtcgggata
      421 gcgcgtgcga agtgcgtcgg cggagaggac gacggcgtcg taggggacgt cgggcggtgc
      481 ggcggccgcg agccacgcgt cgagctttgc gcgttcgagc gtgatgcccg cgcgttcgcc
      541 ttcgcgagag gcgagcttgg cgcgcagtgc ttcgtacgtc gggcgagccc acggcgtcgt
      601 caccgcgcgg atcgcgcgcc agcctcggtg ccaagcttga tcggaggcgt gccagcgcgc
      661 gtggcgcacg gtggagcgca gtcgttcgag gagcggcggc gacgatgcca tcagcggagc
      721 tccatgatgt acaccggctg ctgctcgtcg tcacgacgcg cgtggatccg ccagcccgca
      781 ttgcgcgcga gcgcttcgat cgacgagaga gagggagcgg tgcggacgac gagcgggccg
      841 aacgcgcggc gaagtagggg cgacgcgatg ccgtcggcgt gagcagcgat ccggcgtgcg
      901 ctcgcggctg ggtcgagctc gacgatcatg agggagccgg cgacgacacg gcggagctcg
      961 cgcagcgtcg cggcgcggtc gcgcacgtgg cgcagcgacg acaggcacat cgcggcatcg
     1021 cacgagtccg tcgcgagcgg cagggcttcg ccggaggcgc ggacgacgtg gaggcccggg
     1081 tggggacgcg cgaagtcgcg actcgggtcg acggcgatga cctggagtgc ggggaagcgg
     1141 gcgagggcgc gtcgcgcgaa ggcgcccggg ccgaatccga gatcgagcag gcatttcgcc
     1201 tgggtgagcg cgggccagcc ggcgagcagg cgatcgtcga ggtcgccgaa ggctgggcgg
     1261 gcgaggtcgg cgtaccgccg cgcgctactg ccctcgaacg gagcgcggta gaggacgcgg
     1321 tcgagccagg ccgtgacggt cacgggcgca ccataccccg tgtgaaacgc cacacacacc
     1381 gtgacgtcgc cggtgatacc gtcgcccgcg tgaggggccc acgttgtttt gtcgtcgtcc
     1441 tcgcgtttgc cgctggctgc ggcgactcga tcgatgttcc tgatcgctgt aacccgctcg
     1501 gtggccaagg ctgcatgctg ccgtggccgt cgtcggcgta tgaagtcgac gatccgtcga
     1561 cggtgaccgg ccgccgcgtc gaagtgccga tcgaatcgat gccgaagaac ggcgacgaca
     1621 tctccgtcga tcccgtgtgg ctcaatcgct gggacggctt ctcgccgagc ggcccgatgc
     1681 tcgcggcgtt tgcgagcggc gtgtcgcccg aaggcttgcc gagcttcaag aatcccgacg
     1741 aatcacttgc cgcggactcg ccgatcatcg tcgtcgatat ggcgacgggc gagcgcgcgc
     1801 cgttcttcgc cgaggtcgac cagaacacga aggacgtcct caagcgcaac ctcatcattc
     1861 gcccgctcgc gcgcctgcac acgaatgcgc actacgcggt cggtatcacg aagaaagtga
     1921 aagcggccga cggcggcgag ctctcgatct ccgaaggctt cgcggccgca ctctccggcg
     1981 acggcgatca tcctcgcttt catccgtcgg cgtacaagga catcttcgcc gcgctcgagc
     2041 aggctggcgt cgcaaagacc gacctcgcgc tcgcgtggga tttccacacc gcgtcggacg
     2101 acttcatccg ccgcgatctg acgacgatgc gcgacgccgc gatcaccgcg atgggcacga
     2161 acggcgcgaa cctgtcgttc accgtcacga accaaccggc cgcaaccggc atctacaagg
     2221 cgtatacggg cacgttcaag tcgcccgcgt ttctcacgaa cggcgaagcg gacgagtcag
     2281 tcatgcgtcg cgacgcgaag ggcatcccgc aaatgcaggg catgtgggac gcaccgtttg
     2341 cggcgctcgt gcccgactgc gtgaagacga tgccgctgcc gcgtccgacg atcgtgttcg
     2401 ggcacggcct gttcggctcc gcgtccgagt acctcaagga caacttcacg atcgagctcg
     2461 cgcagtccaa ctgcttcatc atcgtcgctg gcgatttcat cggcttcacg tcgcgccagt
     2521 tcgcgctcgc cgcgctcgcc gtcaacgata tgaaccgcgg cacgcaggtc accgagaagc
     2581 tgacgcagtc gctgatcgac ttcatcacgc tcgagacgct gacgcgcaac cagctcggcc
     2641 aacacgccga gttccagtac aacggccagt cggtgatcga tccggcgcag acgtattacg
     2701 tcggcggatc gcttggcggg atcatgggca acacgttcat ggcgtacgac ccgaacatca
     2761 agcgcggcgt cctcgcggtg ccgggcggcg tgtggtcgat gctcatcgag cgctccttcg
     2821 cgtggcacgc gctgatcggc gcctcgcagg gcagctatcg cgacgccgag gtgtacgagc
     2881 tcaacctcgc gctgttcggc atggccttcg aaccgatcga tccgatctcg acggcggcgc
     2941 acgtcatcaa ggatccgctg ccgggcgtgc ccgcgaagaa catcctcatc tggtacacgc
     3001 tgggcgattc gctggtctcg aacatcacga cggagatgat cgcgcgcgag atgggcatgg
     3061 acctcttgtc gcccgaggtg aagcccgtct ggagaatgcc cggcaaggca ggcccgctcg
     3121 tcaacggagt cgtgaactac aacgagcacc cgacgccgct gccgcccgag acgaacatcc
     3181 caccgtcggc ggacaacggt acgcactcgg gcgtcaatcg aaaccagtcg gcgttgcgcc
     3241 aggtctacga gttcttgctg caaggacagc aagtcgtcca gacctgcggt ggtacgacac
     3301 cggctccctg cgactgcacg accggcgcgt gcgactgatc accggcgggt aggatcgagc
     3361 cgcgtggtac cctgggcacg tgcaggggat ttgtcgcgct ctcgtgatcg gcgtggtggt
     3421 cgcggcgtgc ggtaacccat cgcgcaacga caacgacaac gtccgtcccg acgcgttgaa
     3481 ccaatgcacg atggacggtg cgcatcgctg caacggctcc tcgtacgaga cgtgcaccgg
     3541 cggccagtgg atgagcgcgc tcgattgccc cgcgttctgc gacgaagtgc gcggctgcgt
     3601 cgagtgtcag gccggtcaga cgtactgcaa aggcggaaac gtctggcggt gcgccgacga
     3661 cggaaccggc ggcatgcaga cgcaggaatg cacggggatc aacgtgtgcg acgctggctc
     3721 gtgtgtcgac gcgtgcgcga cggcggccgt caacaagagc tacatcggct gtgactactt
     3781 tgccgttgat ctcgataacg cgctcgagac gaatggcacc tcgaatggct cgtgcccagc
     3841 gggccagacc tccgagatgc tcaacgtctg cttcacgcag atgggcggtg gcacggtctt
     3901 gggccagtgc gatccgcagg acgacggcac cgcaccacac acgtgtcccg cgggcacgac
     3961 gtgtcagtcc aagctcgtct gcgcgacgga tgcgcagcac ggagcgttcg ccgtcgtgat
     4021 ctcgaacccg caggcaaagg acgcgcacgt caccatcacc ggcccccaag gcacgcagat
     4081 catgaagacg gtcaccgcgg gacaggtgct gccgattccg atgcagcagg gcactggcat
     4141 ccccgatcaa tcggtcgaag gcaccggcaa gttcaggaag gcgtatcgaa tccagtcgga
     4201 cattccgatc gtcgcgtatc agttcaaccc gctcgacaac tcgtcggcgg tgttctcgaa
     4261 cgacgcgtcg ctgctgatcc cgtcggcgac ctacgacacc gactactacg ttctgaccga
     4321 gccgacgctc gaccgcagaa actcatcggg cggcttcaag gatccgtatt acgggttcat
     4381 gacggtcgtc gcgtggcaag acggcacaca gatcgcggtg acgccgacgg ccgcgatcat
     4441 gccgagccaa acccagagcg cgatcgccgc cggcacgacc gcgacattta cgctcaacgc
     4501 gttcgaagtt ctccaactcg aggcgtctgg aagcggcgat ctcacgggca cgcacatcac
     4561 gagcccgaac atgatgtcgt tcggtgtctt cgccggtcac gaggcgacga cgctcgacga
     4621 tccgcgcgtc acgagcccga atccgtcgaa gtacccggcc ggtccaggct acgccgacca
     4681 cctcgaggag atgctgtttc ccagctcgac gtggggcaag agcttcgcga tcgcgcgcac
     4741 gaagccccgc atcaacgaga ccgatatcct gcggatcgtc gcgcagatgc cgggcaccaa
     4801 cgtgtcgttc acgccgccgc tcgcggcacc tggagcgccg tcggcaaccc ccgggcccgt
     4861 cgtcggaacg tgccctgcgc tcgacgctgg gaagttctgc gacgtcttca tcgccggcga
     4921 cgtcgagatc accgcggaca agccgatcct ggtcggccac atgatggagt ccgacagctg
     4981 gatcggtaac tcgggcctga tccccggcgc caccggcgac ccctcgctcg cgattgcgac
     5041 gccgaccgag cagttccgca aggagtacac gatcttggtc cctgcggcgt acgccgagaa
     5101 ctacgtgtcg atcgcggcgg cggccaccgg cggcgtgacc gtcgagcgca tcctgccaaa
     5161 ccccatgtcg attccggtga cgctcgcgac gttccccggc ggcggtaccc accgggcggc
     5221 acgcgtcccc ctgtcggcgg ggcagtacaa gatcacctgt gccgacggct gcggcatcgc
     5281 cgtctacggc tactcgcccc aggtctcgta catgttcgcg ggcggtctcg acctcaagcc
     5341 gatcgtgatc ttctgatcgc ctccgtgcgt ttgatcaccg cgcgtttcta gccaagtgat
     5401 aggttcccgc ccgtggaggg acctatgcgt tcatgggtga agtgcatcct cgttgcagcg
     5461 ttggccgcgt gcggcccgag ctcgaaccac ggcgatgacg gcgtcgacgc ggccgtcgat
     5521 tgcaaacccg aaggtgcgca tcagtgcctc ggctcgacgt atcaaacgtg caccggcggc
     5581 acgtggcaga ccgcgctcga ttgccaatca gcgtgcatcg aggatctcgg ttgcgtcgac
     5641 tgtcatcccg gcgagcagtt ctgcaaagac ggcgacgtct ggacgtgcgg tcccgacggc
     5701 atgccgggca gcgtcacgca ggcatgcacc ggtgcaacga cgtgcgcggg cgcgacgtgc
     5761 gtcgatgcgt gtgccgaagc agcagcgagc aagagctaca tcggctgcga gtactgggct
     5821 gtcgatctcg acaacgcact cgaagtgctc ggcggtccgc ctccgttcgg cgcgccgtgc
     5881 aacgacacga cgtttggcta cggcaccggt gtcgccgcga cgcgcgatgt ctgcttcaag
     5941 tcgcagggca acacgacgac gtacgccggt ctctgcgatc cgccgaacaa cgcgtgcccg
     6001 acgggctaca cgtgtcagtc caagagcgtc tgcatcttga acgcgcagca atcgccgttc
     6061 gccatcgtcg tctcgaatcc gcagcccaaa gacgcgagcg tcacggtcac cggccccgcc
     6121 ggccagacgg tcacgaaagt gattcccgcg ggccaggtcg tgccgatcct tccgcaagcc
     6181 ggcaacgcga ttcccgacgc ttcgatcgat ggcacgatca agacgcgcgg cgcctacaag
     6241 gtcgtcgctg atcgcccgat cgtcgcgtat cagttcaacc cgctcgacaa cgtcgacgtg
     6301 ttctcgaacg acgcgtcact gctgatcccg cgcaccgcgt tcgacaccga ctactacgcg
     6361 atgagctacc cgacgctcga tcgccgcacc gtcacgggct cgacgatccc gcagcacaac
     6421 tactacggct acctcaccgt ggtcgcgtgg caggacaaca cgcagatcga ggtcacgccg
     6481 acgaccgcaa tccaagccag cgcgacgcaa acgacgatcg cagccggtgt gacgaccgcg
     6541 ttcacgctca acgcgttcga agtgctgcag ctcgaagcag cgccgcccgc gggcgatctc
     6601 acgggcacgc atatcgcgag cacgaacatg atgtcgttcg gcgtgttcgc gggccacgaa
     6661 gcgacgaact tcggcgagac gacggcgccc gactcgacgc acacgtccgg accatgctgc
     6721 gccgatcacc tcgaagagat gatgttcccc agctcgacgt ggggcaaaga gttcgcgatc
     6781 tcgcgcagcc aacagcgcac gaacgagccc gacatgctgc gcatcctcgc gcagaagccg
     6841 aatacgacgc tgacgttcaa gccggcgccg gcgtcgggca cgtgcggtac gctcaacgcg
     6901 ggtcagttct gccaggtgaa gatcagcggc gacaccgaga tcgtcgcgac ggagccgatc
     6961 ctcgtcggtc actacctcga agcatcgatg tggcgtgaca acccgctgtt cggcatcccg
     7021 acgtgggtgg gcgaaggcga tccctcgatg gcgatcgcgg tgccgaccga gcagtaccgc
     7081 aaggactacg cgatcctcgt gccgtcgcag tacagcaaga gcttcatgtc cgtggcggcc
     7141 gcggcgacgg gcggcgtgtt cgtcgacggt acggccgtca cgctcgcggc gttcccgggt
     7201 ggcggcacgc accgcgcagc tcgcgtgatg ctgacgccgg gtgcacacaa ggtcacgtgc
     7261 cccgatggtt gcggcatcac cgtgtacggc tactccgacg ccgtctcgta catgttcgcc
     7321 ggcggcctcg atctgaagcc gatcgtgatc ttctaga
//
LOCUS       DATLBZ010000366         9529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2665516, whole genome shotgun sequence.
ACCESSION   DATLBZ010000366 DATLBZ010000000
VERSION     DATLBZ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2665516"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VMZ53_18700"
     CDS             <1..1357
                     /locus_tag="VMZ53_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30550.1"
                     /translation="LTGNPVGTDAMQLTGNWSPRMGVIWDPTEEGRSKLYVNWGRYYE
                     GIPMDINDRSFGGEVSVQQTFSAADCGKIDPMTGFADGTKCLTTMASPQDEQLIGSSG
                     TLVAPGIKAQYMDEGMIGGEIALPDNIVFGAILQGRRLGRVIEDVSTDGANTYIIANP
                     GEFSARDEQELQQRIDATDDKAQRARLENQLKLFKGIRQFDKPVRDYAAIELNLSRKF
                     QTGLYLAGSYTYSRTEGNYPGLVSYDNGQIDPNISSQYDLIELLANRRGRLPQDRPHY
                     FKLDAYRDFDLGSKGRLGQLTVGTRIRALSGIPRNALASHYLYGADESYLLPRAQLGR
                     TQFEHGIDVHVAYKRKLTDKTSAELYVDVFNLYNRQGTFRVDETYAPQFSLAGGGGGG
                     IEQNVNPISGGTYEDLVWAKTLDRNGGETSTPAGRNPNFGNTTARYAPASAQVGFRVT
                     F"
     gene            1424..2728
                     /locus_tag="VMZ53_18705"
     CDS             1424..2728
                     /locus_tag="VMZ53_18705"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HUS30551.1"
                     /translation="MRPIRKIVVLGANGAMGSGSGAVFASAGIPTVFLARTLEKAEAG
                     RARAEQLSKGKVAPKSIGIGTYDADLVNQVAEADLIFEAVAEDIAVKKQIFELIDRVK
                     GPGAITATVSSGLSIAQMCEGRSEEFKKNFLGVHLFNPPTIIVGTEIIPHRETDRAVV
                     ELMRQVLEATFGRVVIECADTPAFAGNRIGFKLLNEVAQLAQEHGVAYMDQLVGTHTG
                     RALPPLATIDLVGWDVHKAIVDNLHASTNDEAHSCFALPMYMQRGIERGHLGRKTKDK
                     GGFFRMEGKGADAKHFVLDTVTGDYRPLDEVRPPVPAFIERMKAAIKSGSHAEAFDVL
                     CREQGKDATILRRVMLGYISYALGRVGEVVKHARDVDRIMGFGFNWAPPSVLVDAIGA
                     PRTIELLDAEQLPIPAVIAQAAQAGSPLCDQTIEPARFFVAA"
     gene            2743..3324
                     /locus_tag="VMZ53_18710"
     CDS             2743..3324
                     /locus_tag="VMZ53_18710"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS30552.1"
                     /translation="MRVFNADYVEHATLRDGTPVLLRLVEPQDKALLQRGFENWSAES
                     RYARFFVPKLRLSDDELTYLCDVDQESHFALGAIREGDGEPRGLGIARFIRLPDVDGE
                     AVTAEAAIAVSDEMQGKGLGRILFERLVAAASERGIERFRCDVLGSNSSMKTLIDSLA
                     PVHRTEVGGGVMSIDFALTDANLYGLFKAAAAK"
     gene            complement(3847..4620)
                     /locus_tag="VMZ53_18715"
     CDS             complement(3847..4620)
                     /locus_tag="VMZ53_18715"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HUS30553.1"
                     /translation="MNLDAYFARIGWRGPRSATGEVLAGLLAHHMRAIPFENFDVLLG
                     APPSLDLEALEDKLVTRRRGGYCYEHTTLLAAALEQLGFAPKRHAARVIMLTPKESAP
                     RTHMFLTLDGLVLDPGFGAIAPRSPVPIDGEAGAHRLVREGDERVLVFGDQRLWTSTC
                     EPLPHIDFVMANHFTATFPASPFVNRIMARAFTDDGEVRISNRDVTRTRGGQTETFVL
                     PDRRGLREVVAAHFGFDLPALDSVCVPSIPEWASPPSWP"
     gene            4726..4932
                     /locus_tag="VMZ53_18720"
     CDS             4726..4932
                     /locus_tag="VMZ53_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30554.1"
                     /translation="MRTPDDDETLPEISTEELTGVQGGAGFGDSLMQMLPLMMIMRQR
                     QAPPPPPAVAAPPPPTDGDWTRVA"
     gene            4991..6022
                     /locus_tag="VMZ53_18725"
     CDS             4991..6022
                     /locus_tag="VMZ53_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30555.1"
                     /translation="MRVHLALLLAVAACGDDSGGTNPDAPLGGNGDGGDGSMDVPCSY
                     TEMSDPGNASSTTPEVTNITFATKAVLCGKVDTGHFDATEQQVDQDIYKLTFAADTDL
                     IIHFTGAASGLDELAVQVAQTAARAGYGSWTHDHGALVAHVTAGDWYVTAGAFKASDA
                     TAAFPYKIVIETDTPGRCAHLTTGGFTEAGDGTNNVGNDMIEYDSSADPTKRLTTGST
                     DTPEATGVLTAPGMQYRLTGTAANVNASDDYQDRDTYEFMTDAATNELSVRLNWDGTT
                     RDFDFLAVPQNSVKLVGAGLDNATTADEFETFAVKPSSTYWIWVGAYDGAALPTNYDI
                     TVCASTYTP"
     gene            6072..6692
                     /locus_tag="VMZ53_18730"
     CDS             6072..6692
                     /locus_tag="VMZ53_18730"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HUS30556.1"
                     /translation="MQIPVEITFRDIDRSEAVEARIREWAEKLERIYDRIVRCEVVVD
                     DPHRHHHKGHQYYVRVRLTVPGTEIVSSHPGNDDRAHEDVYVAVRDAFQATKRQLEAY
                     VRKSLHRGDVRREGNGASHGRVTFLDVEKAWGWLEPDDGRRIYFHRNSVRGGMDNVNV
                     GDEVRFTEEMGNEGPQASTVEVIGTHGHHVMNEPAAVDASSAGGRR"
     gene            6743..7183
                     /locus_tag="VMZ53_18735"
     CDS             6743..7183
                     /locus_tag="VMZ53_18735"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUS30557.1"
                     /translation="MALKRSSTAVWHGTGPKGSGKISTLSGALTDQPYSVNSRFQSED
                     GKAGTNPEELIAAAHAGCFTMALSFQLTNAGFEPTELRTEAVVSMEKQDPGWTITNIN
                     LDVTGKVPNVTAEKFEELAQTAKKGCPISRALAATNITMSAKLS"
     gene            7180..7557
                     /locus_tag="VMZ53_18740"
     CDS             7180..7557
                     /locus_tag="VMZ53_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30558.1"
                     /translation="MSGEDRRLACPECGNRQLEGRERCTSCGYIDGLLSLDNPEHVDL
                     LRDIDQARFDKHEKRMRLLAVVVAMVAVFALWAVPGFWKARQESVALPFLIDQWALMI
                     VIAFGITLVMKRTRPKPLFPYVS"
     gene            complement(7554..8018)
                     /locus_tag="VMZ53_18745"
     CDS             complement(7554..8018)
                     /locus_tag="VMZ53_18745"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUS30559.1"
                     /translation="MSISDRILPLCELLLGAAYADKELKDQEKDKVRELLEELSSDLP
                     TDVELKLVSFDPKGWNMKEAAHAFTGDSEDDRKKVLQLVAAVNEADDELDIAEDEYLR
                     NLAGALGLPASALAGMTVEVEAEDLKPIFEKVRKGPPPPPKAKEKSVDVDMD"
     gene            8089..8418
                     /locus_tag="VMZ53_18750"
     CDS             8089..8418
                     /locus_tag="VMZ53_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HUS30560.1"
                     /translation="MKDRVDADERALVCAACELRITDEDYRIEMSGAHEHTFVNPAGM
                     VHHIACFSVAPGCVHLGTTESAFSWFPGWTWQVAACARCRAHLGWIYRNAGQQFHGLI
                     TAAIRPR"
     gene            complement(8419..8721)
                     /locus_tag="VMZ53_18755"
     CDS             complement(8419..8721)
                     /locus_tag="VMZ53_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30561.1"
                     /translation="MKTITTVELELVTGGAYPLGTIDKPGSSGGNDQLLSTLTGIQSS
                     LKDLSKNQNNQGLFGGQNGLLFVTALMLSQRNNTNTVVYQQGGGCAPRHGFRFRIW"
     gene            8870..9469
                     /locus_tag="VMZ53_18760"
     CDS             8870..9469
                     /locus_tag="VMZ53_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30562.1"
                     /translation="MRALALVAAAPILLLAGSAKADSFLDIEGGLTTPLGDDDWNNTV
                     ESSPKLAVHAGSVPGTVGGMVSVDWTPYNTDAQADFPGTDIAANRFRILANLAFMSPV
                     KAKLMLTGRVGIGADIQHASAEGNIFGVHFETSDTDVGIGFEVAAGLWARVGGVAIGG
                     ELALPISHHDQDNQQGDIDFIYDTFDLDLMFGVRLMTDN"
BASE COUNT         1769 a         3116 c         3064 g         1580 t
ORIGIN      
        1 gctgaccggc aatccagtcg ggaccgatgc gatgcagctg accggcaact ggtcgccgcg
       61 catgggcgtc atctgggatc cgaccgaaga gggtcgcagc aagctctacg tcaactgggg
      121 tcgctactac gaaggcatcc ccatggacat caacgatcgg tcgttcggcg gcgaggtgag
      181 cgtgcagcaa acgttctcgg cggcggactg cggaaagatt gatccgatga cgggcttcgc
      241 cgacggcacg aagtgcctga cgacgatggc gtcgccgcaa gacgagcagc tgatcggctc
      301 gagcggaaca ctcgtcgcgc cggggatcaa ggcgcagtac atggacgagg gcatgatcgg
      361 cggcgagatc gcgctgccgg acaacatcgt gttcggcgcg atcctgcaag gccgccgcct
      421 cggccgcgtg atcgaagatg tctcgaccga cggcgcgaac acgtacatca tcgccaaccc
      481 gggcgaattc tcggcgcgtg acgagcagga gctgcagcag cgtatcgacg cgaccgacga
      541 caaggcgcag cgcgcgcggc tcgagaacca gctgaagctg ttcaagggca tccgccagtt
      601 cgacaagccg gtgcgcgact acgcggcgat cgagctgaac ctgtcgcgca aattccaaac
      661 gggcctctac ctcgccggct cgtacacgta ctcgcgcacc gagggtaact acccgggcct
      721 cgtgtcgtac gacaacggcc agatcgaccc gaacatctcg tcgcagtacg acctcatcga
      781 gctactcgct aaccgccgag gtcgcttgcc gcaggatcgc ccgcactact tcaagctcga
      841 cgcgtatcgc gacttcgatc tcggcagcaa aggccggctc ggtcagctga cggttggaac
      901 gcgcatccgc gcactgtcgg gcattccacg caatgcgctc gcgtcgcact acctctacgg
      961 tgccgacgag tcgtacctcc tcccacgtgc gcagctgggg cggactcagt tcgaacatgg
     1021 gatcgacgtt cacgtcgcgt acaagcgcaa gctcaccgac aagacctcgg ccgagctcta
     1081 cgtcgacgtt ttcaatctct acaaccgcca aggcacgttc cgcgtcgacg aaacgtacgc
     1141 gcctcagttc tctctcgccg gtggcggtgg tggcggcatc gagcagaacg tcaatccgat
     1201 ctcgggcggt acgtatgaag acctcgtgtg ggcaaagacg ctcgaccgaa atggcggcga
     1261 aacgtcgacg ccggcgggtc gcaatccaaa cttcggcaac accaccgcgc gctacgcgcc
     1321 cgcgtcagcg caagtgggct tccgcgtcac attctagtca ggttctttcg ttcagctttc
     1381 aaggggatcg cgcggtacgc gatttgctgt caactaggga cccatgaggc ccatccgcaa
     1441 gatcgttgtc cttggtgcta acggtgcgat gggatcggga tcgggggcgg tcttcgcctc
     1501 tgccggcatt cccacggtct tcctcgcgcg cacgctcgag aaagcagaag cgggacgcgc
     1561 gcgcgccgag cagctctcca aaggcaaggt cgcgccgaag tcgatcggca tcggcacgta
     1621 cgacgcggac ctcgtcaacc aggttgccga agcggacctc atcttcgaag ccgtcgccga
     1681 agacatcgcc gtcaagaagc agatcttcga gctcatcgat cgcgtgaagg ggccgggcgc
     1741 gatcaccgcg accgtatcgt cgggcctctc gatcgcgcag atgtgcgagg gccgcagcga
     1801 ggagttcaag aagaacttcc tcggcgttca cctattcaac ccgccgacga tcatcgtcgg
     1861 caccgagatc atcccgcacc gcgagacgga ccgcgctgtc gtcgagctca tgcgccaggt
     1921 cctcgaagcg acgtttggcc gcgtcgtgat cgagtgcgcc gacacgccgg ccttcgcggg
     1981 caatcgcatc ggcttcaagc tcctcaacga ggtcgcgcag ctcgcgcaag agcacggcgt
     2041 cgcctacatg gatcagctcg tcggcaccca caccggccgc gcgctgccgc cgctcgcgac
     2101 gatcgacctc gtcggctggg acgtccacaa ggcgatcgtc gacaacctgc acgcgagcac
     2161 gaacgatgaa gcgcacagct gcttcgcgct gccgatgtac atgcagcgcg gcatcgagcg
     2221 cggtcatctc ggccgtaaga cgaaagacaa gggcggcttc ttccgcatgg aaggcaaggg
     2281 cgccgacgcg aagcacttcg tgctcgacac cgtcaccggt gattaccgcc cgctcgacga
     2341 agtccgccca cccgtgcccg cgttcatcga gcgcatgaag gcagcgatca agagcggcag
     2401 ccacgccgaa gcgttcgacg tgctgtgccg cgaacagggc aaggacgcga cgatcctgcg
     2461 ccgcgtaatg ctcggctaca tcagctacgc gctcggacgc gtcggtgaag tcgtcaagca
     2521 cgcgcgcgat gtcgatcgca tcatgggctt tggcttcaac tgggcgccac cgagcgtcct
     2581 cgtcgatgcg atcggtgcgc cgcgcacgat cgagctcctc gacgcggagc aactcccgat
     2641 tcccgccgtc atcgcgcaag ctgcgcaagc aggctcgccg ctctgcgacc agacgatcga
     2701 gccagctcgc ttcttcgtcg ccgcttagct accctcgcgc gcatgcgcgt gttcaacgcc
     2761 gactacgtcg agcacgcgac gctgcgcgac ggcacgcccg tcttgctccg gctcgtcgag
     2821 ccgcaggaca aggcgctgct ccagcgcggc ttcgagaatt ggtccgcgga gtctcggtac
     2881 gcgcgcttct tcgtgccgaa gctgcggctc tcggacgacg agctgacgta tctctgcgat
     2941 gtcgatcagg agagccactt cgcgctcggc gcgattcgcg aaggcgacgg cgagcctcgc
     3001 ggcttgggca tcgcgcgctt cattcggctg cccgatgtcg acggcgaggc ggtgacggcg
     3061 gaagccgcga tcgcggtctc cgacgaaatg cagggcaagg gactcggtcg catcctgttc
     3121 gagcgcctcg ttgcggcggc gagcgagcgc ggcatcgaac ggtttcggtg cgacgtgctc
     3181 ggctcgaact cgtcgatgaa gacgctgatc gattcgctcg cccccgtgca tcgcaccgag
     3241 gtgggcggcg gcgtgatgtc gatcgacttc gcgctgacgg acgcgaacct ctacggcttg
     3301 ttcaaggccg ctgctgcgaa atgatcctgt tcacgatcgg ccactcgaat cggtcgtatg
     3361 gcgagctcgt cgaagcgctg cgctcgcacg gcgtcgagac tctcgtcgac atccgccact
     3421 tcccgcgatc gcgtgcgaac ccgcagttca atgccgatgc gctcgcgaag cggttacccc
     3481 gcgatggcat acgcttcgtc gagtcgcgga agctcggtgg caggcgcagc acgtcgaaga
     3541 cggcgaagcc gacgcgaaac gccggctggc agaatcgcgc gttcaagaac tacgcggact
     3601 acgcggagac gcgcgaattc caggcagcgc tgcgtgcgtt gttggcgcgc gctcgcaagg
     3661 agagctgcgc gatcatgtgc gccgaggtgc tgtggtggcg ctgtcaccgt cggatcgtcg
     3721 ccgactacgc gatgacccgc ggcgccgacg tgtttcacat cttcacggcg acgaagatcg
     3781 aggaagcgaa gcgcacgcca ttcgcgcgtc gcgatccgaa gacgaagacg ctgcgctatc
     3841 cagcccctag ggccacgacg gcggcgaagc ccactcggga atcgaaggca cgcacaccga
     3901 gtcgagcgcc ggcaagtcga agccgaagtg cgccgcgacc acctcgcgca agccgcgccg
     3961 gtcgggcagc acgaacgtct cggtctggcc gccgcgcgtc cgcgtgacat cgcgattcga
     4021 gatccgcacc tcgccgtcgt cggtgaacgc gcgggccatg atgcgattga cgaacggcga
     4081 cgccggaaac gtcgcggtga agtggttcgc catcacgaag tcgatgtgcg gcagcggctc
     4141 gcaggtcgat gtccacagcc gctggtcgcc gaagacgagc acacgctcgt caccttcgcg
     4201 gacgagtcga tgcgcacctg cttcgccgtc gatcggaact ggcgagcgcg gagcgatcgc
     4261 accgaagccc gggtcgagca cgagtccgtc cagcgtcaag aacatgtgcg tgcgcggcgc
     4321 gctctccttc ggcgtcagca tgatcacgcg cgcggcatgc cgtttcggcg cgaagccaag
     4381 ctgctcgagc gcggcggcca acagcgtcgt gtgctcgtag caatagccgc cgcgccggcg
     4441 cgtcacgagc ttgtcttcga gcgcttcgag atcgagggaa ggcggagcgc cgagcagaac
     4501 gtcgaagttc tcgaacggga tggcacgcat gtgatgggcg agcagcccgg cgagcacctc
     4561 gcccgttgcg ctccggggac cgcgccagcc aatccgggcg aagtacgcgt cgaggttcac
     4621 cccaccatcg tatccccggg tatgcggagc gagccaacgc ggccgcgcaa gaccgcgtac
     4681 gcgcgtcgaa acctgctggc atacaccgtg ctgtctacag tcgacatgcg cacccccgac
     4741 gacgacgaaa cgctccccga gatttcgacc gaagagctca ccggcgtgca aggcggagcc
     4801 gggttcggcg actcgctgat gcagatgctg ccgctcatga tgatcatgcg gcagaggcaa
     4861 gcaccgccac ctccgccggc tgttgccgca ccgccgccgc cgacggacgg cgactggacg
     4921 cgcgtcgcgt agcaaatcgc acgtcgcccc tacatcgcat cgcgttcgca cgtcggcgta
     4981 gaatgggcgc atgcgtgtcc atctcgcttt gcttcttgcg gtcgcggcgt gcggtgacga
     5041 ctccggtggt acgaaccctg acgcgccgct cggcggaaac ggcgacggtg gcgatggctc
     5101 gatggacgtg ccgtgctcgt acacggagat gtccgatccg ggtaatgcgt cgagcacgac
     5161 gcccgaggtc acgaacatca cgttcgcaac gaaggccgtg ttgtgcggca aggtcgacac
     5221 cggccacttc gatgcgaccg agcagcaggt cgatcaagac atctacaagc tgacgttcgc
     5281 ggccgacacc gatctgatca ttcacttcac gggcgccgcg tcgggcctcg acgagctcgc
     5341 cgtgcaggtc gcgcaaaccg ccgcgcgcgc gggctacggc tcgtggacgc acgatcacgg
     5401 cgcactcgtc gcgcatgtca ccgcgggcga ttggtacgtc acggccggcg cgttcaaagc
     5461 gagcgatgcg acggcagcct tcccgtacaa gatcgtcatc gagaccgaca cgccaggccg
     5521 ctgcgcgcat ctcacgaccg gcggcttcac agaggcaggc gacggcacaa acaacgtcgg
     5581 caacgacatg atcgaatacg actcgtcggc cgacccgacg aagcgcctca cgaccggctc
     5641 taccgatact cccgaggcca ccggcgtcct cacagcgccc ggtatgcagt accggctcac
     5701 tggcacggct gcgaacgtca acgcgagtga cgactaccag gatcgggata cgtacgaatt
     5761 catgaccgac gcggccacca acgagctcag cgttcggctc aattgggatg gcacgacacg
     5821 agatttcgac ttccttgcgg tgccccagaa ttcggtgaag ctcgtcggtg cgggcctcga
     5881 caatgcgacc acggctgacg aattcgagac gttcgccgtg aagccgagct cgacgtactg
     5941 gatctgggtg ggcgcgtacg atggcgccgc gctgccaacg aattacgaca tcacggtctg
     6001 cgcttcgaca tacacgccct gatcagaaag gtccgcatgg ccgtggcgca cgcgatgcac
     6061 gccctaagat catgcagatc cccgtcgaaa tcacgtttag agacatcgat cgaagtgagg
     6121 ccgtcgaagc tcggatccgc gaatgggcgg agaagctcga gcgaatctac gaccggatcg
     6181 tgcgttgtga ggtggtggtc gacgatccgc atcggcacca tcacaagggt caccagtact
     6241 acgtgcgcgt gcggctgacg gttccgggca ccgaaatcgt ttcgagtcac ccgggcaatg
     6301 acgaccgcgc tcacgaggac gtctacgttg cggtgcgcga tgcgttccaa gcgacgaagc
     6361 gccagctcga agcctacgtc cgcaagagcc tgcatcgcgg cgatgttcgg cgcgaaggca
     6421 acggcgcttc gcacggtcgc gtcacgttcc tcgacgtcga gaaggcgtgg gggtggctcg
     6481 agccggacga tggtcgtcgc atctacttcc accgcaacag cgtgcgcggc ggaatggaca
     6541 acgtgaacgt cggtgacgag gtgcggttca ccgaagagat gggcaacgaa ggtccgcagg
     6601 ccagcaccgt cgaggtgatc ggcacacacg ggcaccacgt gatgaacgag ccagctgcag
     6661 tggacgcgag ctcagcagga ggccgccgct aggttccgca cgtcggtagc gtgccggccg
     6721 actcccggat ataccgccgg gcatggcact caaacgttca agcaccgcgg tgtggcacgg
     6781 cactggcccg aagggctcgg gcaagatttc gacgctgtcg ggcgccctca cagaccagcc
     6841 ttacagcgtg aacagccgct tccaatcgga agacggcaag gccggcacca accccgagga
     6901 gctcatcgcg gcggcgcacg ccggctgctt cacgatggcg ctctcgttcc agctcacgaa
     6961 cgccggcttc gagccgacgg agctgcgcac cgaggccgtc gtcagcatgg agaagcagga
     7021 cccgggctgg acgatcacga acatcaacct cgacgtgacc ggcaaggtgc cgaacgtgac
     7081 cgccgagaag ttcgaggagc tcgcgcagac cgcgaagaag ggctgcccga tctctcgcgc
     7141 gctcgcggcg acgaacatca cgatgtccgc caagctgtcg tgagcggcga agaccgccgt
     7201 ctcgcgtgtc ccgagtgtgg caatcgccag ctcgaaggtc gcgagcgctg cacgtcctgc
     7261 ggctacatcg acggcctgct ctcgctcgac aatcccgagc acgtcgatct gctgcgcgat
     7321 atcgatcagg cacgcttcga caagcacgag aaacgcatgc gactcctcgc cgtcgtggtc
     7381 gcgatggtcg cggtgttcgc gctttgggcc gtgccgggat tctggaaggc gcggcaggag
     7441 agcgtcgcgc tgccgtttct gatcgatcag tgggcgctga tgatcgtgat cgcgtttggc
     7501 atcacgctcg tcatgaagcg cacgcggcca aagccgctgt tcccgtacgt cagctagtcc
     7561 atgtcgacgt cgacggactt ctctttcgcc ttcggcggag gcggcggacc tttgcgaacc
     7621 ttctcgaaga tcggcttgag atcttcggct tcgacttcga ctgtcatgcc cgcgagcgcc
     7681 gatgccggta gaccgagcgc acccgcaagg ttgcgcagat attcatcctc ggcaatgtcg
     7741 agctcgtcgt cggcttcgtt cactgcagcg acgagctgca gcaccttctt gcgatcgtcc
     7801 tcgctgtcgc ccgtgaaggc gtgcgccgct tctttcatgt tccagccctt gggatcgaag
     7861 ctgacgagct tgagctcgac gtcggtcggc aagtcactgg agagctcttc gaggagctcg
     7921 cggaccttgt ccttctcttg gtccttcagt tccttgtccg cgtacgcggc accgagcaac
     7981 agctcacaca gcggcaggat ccgatccgag atcgacatgg agcggtcatt ctacactcgg
     8041 atggtgctcc tcgatcgaga gcgagaccgc agatcctcac cgcacgcggt gaaagaccgt
     8101 gtcgacgcgg acgagcgggc tctggtgtgt gcggcctgcg agctgcgcat caccgacgag
     8161 gattaccgca tcgagatgtc cggcgcgcac gagcacacgt tcgtcaatcc agcgggaatg
     8221 gtgcatcaca tcgcgtgctt ctcggtagcg cccggctgcg tccacctcgg gaccacggag
     8281 agtgcgttct cgtggtttcc cgggtggacg tggcaggtcg ctgcctgtgc acgttgccgc
     8341 gcgcacctcg gctggattta tcgcaatgcg ggacagcagt ttcacggcct catcacggcc
     8401 gcgattcgcc cgcgctagct accagatccg gaagcggaag ccgtggcgcg gcgcgcagcc
     8461 gccaccttgc tggtagacga ccgtgttcgt gttgttgcgc tgcgagagca tcagcgcggt
     8521 cacgaacagg aggccgttct ggccgccgaa cagaccctgg ttgttctggt tcttgctcag
     8581 gtccttcagc gagctctgga ttccggtcag cgtcgagagc agctgatcgt tgccgccgct
     8641 cgagccgggc ttgtcgatcg tgccgagggg atacgcgccg ccagtaacaa gctcgagctc
     8701 aacagtggtg atggtcttca tggtgggatg ctccttgcga gtgactgacg cttagagcag
     8761 ccggcgtgcc acggccaaag tatcgagatc tcttgggaag gccacctgaa tgctgtctac
     8821 cgccaggtga cattgcgagt cctccggaag acagcgttac ccttgcggca tgcgagccct
     8881 cgctcttgtc gccgccgccc cgattctcct cctggctgga tccgccaagg ccgacagctt
     8941 cctcgatatc gagggcggat tgacgacgcc gctcggcgat gacgactgga acaacacggt
     9001 cgaatccagc cccaagctcg cggtgcacgc cggcagtgtc ccgggcaccg tgggcggcat
     9061 ggtgtccgtc gactggactc cctacaacac ggatgcgcag gcagactttc cgggcaccga
     9121 tatcgcggcg aaccggttcc ggattctcgc gaacctcgcg ttcatgagcc ccgtgaaggc
     9181 aaagctcatg ctgacgggcc gcgtcggaat tggtgcggac atccagcatg cgagcgccga
     9241 ggggaacatc ttcggcgtcc acttcgagac ctcggatacg gacgtcggca tcggcttcga
     9301 ggtcgcggct ggcctgtggg cacgggtcgg cggtgtcgcg atcggcggcg agctcgcact
     9361 accgatcagc catcacgatc aggacaacca gcagggcgac atcgacttca tctacgacac
     9421 gttcgatctc gacctgatgt tcggcgtgcg cctgatgacc gacaactgac cggcaactaa
     9481 aggcgcaacc gcgcgtgacg gcccgtcggt cgaccgcgca gttcttcga
//
LOCUS       DATLBZ010000367         6162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9370822, whole genome shotgun sequence.
ACCESSION   DATLBZ010000367 DATLBZ010000000
VERSION     DATLBZ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9370822"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VMZ53_18765"
     CDS             <1..609
                     /locus_tag="VMZ53_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30563.1"
                     /translation="HYELEGQHAIDHPDAMTYPAARDYVVGNARLHDFFGGKHFGVHA
                     GLDLGAGGAIHGGGFAYDVLLFPIGFAWRFFETSFFTVGPGVGASGATGVLDDAATFP
                     LEARLEVGRGVRLLARGRVTYLSAAPMREDGARFGDELEGMVGLRLGNAYDEYGFPTG
                     NGYFVGASYKEQLGARFVGLMIGYSIDMGTPRGPRRDHAIGL"
     gene            622..1041
                     /locus_tag="VMZ53_18770"
     CDS             622..1041
                     /locus_tag="VMZ53_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30564.1"
                     /translation="MIRWLRLVAIAVVVLSMIGLGIIVGLFLVANSGWIPIKTPDWLT
                     GLFGNKAHDIRLPALIAGWLVAAILLLTMVLWSLFYVWRRRQYESLVNRLERELAKLR
                     NLPFVEPAPLEDLPEQPSMQAARVMSALDDDEGLGPS"
     gene            1041..2738
                     /locus_tag="VMZ53_18775"
     CDS             1041..2738
                     /locus_tag="VMZ53_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30565.1"
                     /translation="MGAAIVLLLVGLGCMAAGVLIGRYYVPDDRQLRRTARHARSYMK
                     ALSYLIARDHETVVNELRTVVEENIEDVEPYFALGAMFRTRGEHERAIRVHQALAVRE
                     RDKRKVRQRAMYELGLDFRQAGMPRRATKAMEEVLLEDPAHEGALRSLAALYEEQARF
                     NESAGLWARLGKRRGEDLSSREHHLLVAAAQAALARDDLESAKHLLKSAQKLSESAHF
                     FAAAAELAAARGNHSGAKDRLQQALIADPALAPHLLPGLIAAEQGLDDNAKSGKRGSR
                     RDDLVDDDAPKLAEGTSGGTKALASGETALATTDGGTLATTAAGTLAMTGAGALATNA
                     AGTLATTGGTSAGRDLGANVVPGTIPLVPDAKPAGTVVDRVIALLADVEAQVGPRLEL
                     ALIRAQLASGSDTRVKDELAQELASKFPDALAARVAAARLALAKGDDKSVRQALEILA
                     GDKGTLAWALRGRWQCSHCGNRPGQFSWRCGQCRRWAALRMETGIEPPPVAPRDRRAS
                     PRSSRPEGLLGAAPDLGLPSPTLDPGLTEDELAVAGTRRSLLNRVGGWFSGAWRRND"
     gene            2766..4250
                     /locus_tag="VMZ53_18780"
     CDS             2766..4250
                     /locus_tag="VMZ53_18780"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUS30566.1"
                     /translation="MTPPPDDDLPGNQGVPRPIDTGPVTSTSPTTPLPGITSGRSRTR
                     DTWFGENAPSRKFLSRWGFPLFVVFIAFLGRKVLLPFVFAFLIAYILAPVVRWMSERK
                     NGTRRMPRGLAIIICYIIFISAVVGFMFLLVPRLSRDVARLGKEAPGLYKKVNDEYVP
                     STARWLERKFPSLAEVKTAPEQQLPVADVPEPPGTAFTVTPLPDGRLALQLTPNGLDI
                     KPLPDGGYHIKTLEFPPEPATLEDKLRGYVKKGIVGLQSKLNDVVRFTQTLIAGFIKG
                     IFLFFFTLMIGAFILIDLEKVHAFMRGLFPANVRDDYDVIIAGMDRGLSGVIRGQLLI
                     CLINGIFTYIGLLIFNVKYGFILATVAGLMSLIPIFGSILSSVPIVTVALASGEKGID
                     IFRGVAMTLWIIGIHFVEANLLNPKIIGSAAKIHPVLVIFALFLGESSFGLVGALLAV
                     PILSAIQVVFMYFYRKRWKDAPRPQTGPMPRPPGEAGPLAPTQR"
     gene            4276..5601
                     /locus_tag="VMZ53_18785"
     CDS             4276..5601
                     /locus_tag="VMZ53_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30567.1"
                     /translation="MRAATLLALCVSSAAYAQAPAPDAPPPGSSDPVLDEQIAQQLVD
                     RAQELLDAKLWLDAKQLAVEARVKSPKGPSAERARYIINIVNQHLDIKEEPPPTPPSE
                     VPPTTTPPTATPDIPQVGDRINPFEPPAKDPEEPPPATPPGNGNPRTAAMIHGGLYGA
                     TIGTTIGVAANSDNPAAAAIPLGIVGGVGVGYLSTKLVARAHWDEAQVRTAGSYSTWG
                     SAIGGLFGGAATGAGDSSPSATGVLVGASIGSTIGAGVGIAFARTHKFTRGDVALTDT
                     LAGIGAVGGLTLGMLMQPVQPEAYAVNSIVGISAGLVVGLVAGPQTNTTPRRMLRVAG
                     LSAAGGAAPFLLYAAIYDSSSASDERVTGLLSSAGLVVGAYLGFRITAKMDEGVDKAD
                     APPDDAPIALVGRSSNGNWALGGIGVQPLSRQLATNNRGMTFTVVGATF"
     gene            complement(5602..>6162)
                     /locus_tag="VMZ53_18790"
     CDS             complement(5602..>6162)
                     /locus_tag="VMZ53_18790"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUS30568.1"
                     /translation="ETAGSARTRPVYSDPRSQKTPLGYLSSLNLVRTAQHTIYIANSY
                     FVPPRRLRKHLKAAVARGVRVVVVTNSLHSTDAWWMYYASLNYYKELLGAGIEVYQYR
                     GTETLHSKTMLVDGKLAVIGSYNLDPRSAASNSESIVTVRDGAAVGELAADFQAMLGY
                     SDPASADISAADWVKAKAFKIAEPLL"
BASE COUNT          992 a         2144 c         2011 g         1015 t
ORIGIN      
        1 cactacgagc tcgagggcca gcacgcgatc gatcatcccg atgcgatgac gtatcccgct
       61 gcgcgcgact acgtggttgg caacgcgcga ttgcatgact tcttcggcgg caagcatttc
      121 ggcgtgcacg cggggctcga cctcggcgcg ggcggcgcga ttcacggcgg cggcttcgcg
      181 tacgacgtgt tgctgtttcc gatcggcttt gcgtggcggt tcttcgagac gtcgttcttc
      241 accgttggac cgggcgtcgg tgcatccggc gcgaccggcg tgctcgatga tgccgcgacg
      301 tttccgctgg aagcgcggct cgaagtcggc cgcggcgtga ggctgctcgc gcgtggacgc
      361 gtgacgtatc tgtcggcggc gccgatgcgc gaggacggtg cccgatttgg cgacgagctc
      421 gaaggcatgg tggggcttcg cctcggcaac gcgtacgacg agtacggctt tccgacgggc
      481 aacggctact tcgtcggtgc gtcgtacaag gagcagctcg gcgcgcgctt cgtcgggctg
      541 atgatcggct actcgatcga tatgggcaca ccgcgcggcc ctcgtcgcga ccacgccatc
      601 gggctgtagt acagtcgaag cgtgatccgg tggctccgac tcgtcgcgat cgcggtggtc
      661 gtcttgtcga tgatcggcct cgggatcatc gtcgggctgt tcctcgtcgc gaactcgggc
      721 tggattccga tcaagacgcc ggactggctc acgggcctgt tcggcaacaa ggcgcatgac
      781 atccggctcc ctgcgctgat cgcaggctgg ctcgttgctg cgatcctgct gctgaccatg
      841 gtgttgtggt cgctgttcta cgtgtggcgc cggcggcagt acgagtcgct cgtcaatcgg
      901 ctcgaacgcg agctcgcgaa gctgcgcaac ctgccgttcg tcgagcccgc accgctcgag
      961 gatctgccgg agcaaccgag catgcaggcc gcgcgcgtga tgagcgcgct cgacgatgat
     1021 gaagggcttg ggccgtcgta atgggagccg cgatcgtcct cttgctcgtc ggccttggct
     1081 gcatggcagc gggcgtgctc atcggtcgct actacgtgcc agacgatcgg cagctgcgac
     1141 gcaccgcgcg ccacgcgcgc tcgtacatga aggcgctgag ctatctgatc gcgcgcgatc
     1201 acgagacggt cgtcaacgag ctgcgcaccg tcgtcgagga gaacatcgag gacgtcgagc
     1261 cgtacttcgc gctcggcgcg atgttccgca cccgtggcga gcacgagcgc gcgattcgcg
     1321 tgcatcaagc attggcggtg cgcgagcgcg acaagcgcaa ggttcgccag cgcgcgatgt
     1381 acgagctcgg cctcgacttc cgccaagcgg gcatgcctcg gcgcgctacg aaggcgatgg
     1441 aggaagtgct gctcgaggat ccggcccacg aaggcgcact gcgctcgctc gctgctctct
     1501 acgaagagca ggcgcgcttc aacgaatcgg ccggcttgtg ggcgcggctc ggcaagcgtc
     1561 gcggggagga cctcagctcg cgcgagcacc atctgctcgt cgccgcagcg caggccgcac
     1621 tcgcgcgtga tgatctcgag agcgcgaagc atttgctcaa gtccgcgcag aagctctcgg
     1681 agagcgcgca cttctttgca gcggccgccg agcttgcagc ggcgcgtggc aatcacagcg
     1741 gcgcgaagga ccgactccag caagcgctga tcgccgaccc cgcgctcgcg ccgcacctgc
     1801 tgccgggcct catcgcggca gagcagggcc tcgacgacaa cgcgaagagc ggcaagcgtg
     1861 gctcgcgccg cgacgacctc gtcgatgacg acgcgccgaa gctcgccgag ggaacgagcg
     1921 gcgggaccaa ggcgctcgcg agtggcgaga ccgcgctcgc gacgaccgat ggaggcacgc
     1981 tcgcgacgac cgccgccggc acgctcgcga tgaccggcgc aggcgcgctc gcgacgaacg
     2041 ccgccggcac gctcgcgacg accggcggaa cgagcgctgg tcgcgacctc ggtgcgaacg
     2101 tcgtgccggg caccatcccg ctggtccccg atgcaaagcc cgcgggcacc gtcgtcgacc
     2161 gcgtgatcgc attgctcgcg gatgtcgagg cgcaggtcgg tccgcgtctc gagctcgcgc
     2221 tcatccgcgc gcagcttgcg tcgggctccg acacgcgcgt gaaagacgag ctcgcgcagg
     2281 agctcgcgtc gaagtttccc gacgcgctcg ctgctcgcgt tgccgccgct cgcctcgcgc
     2341 tcgccaaggg cgacgacaag agcgtgcgcc aagcgctcga gatcctcgcc ggcgacaagg
     2401 ggacgctcgc gtgggcgctg cgcggccgct ggcagtgctc gcattgtggc aatcgcccgg
     2461 ggcagttttc gtggcggtgc gggcaatgcc gccggtgggc agcgctgcgc atggagacgg
     2521 gcatcgagcc gccgccggtt gcaccgcgcg atcgccgtgc ctcgccgcgt tcatcgcgac
     2581 ccgaaggcct gctcggtgct gcaccggatc tcggtttgcc gtcaccgacg ctcgatcccg
     2641 gtctcaccga ggacgagctc gccgttgcgg gaacccgtcg atcgcttctc aatcgcgtcg
     2701 gtggctggtt ctcgggcgcg tggcgccgca acgactgacg cctctggtat accggcccag
     2761 ccgagatgac gccgccgccg gatgacgacc tgcctggcaa ccaaggtgtc ccgcgtccga
     2821 tcgacaccgg accggtaacg tcgacgtcgc cgaccacgcc gctgcctggg atcacgagtg
     2881 gccgctcgcg cacgcgtgac acgtggttcg gcgagaacgc gccgtcgcga aagttcctgt
     2941 cgcggtgggg cttcccactg ttcgtcgtct tcatcgcgtt cctcggccgc aaggttctgc
     3001 tgccgttcgt gttcgcgttc ttgatcgcgt acatcctcgc ccccgtcgtg cgctggatga
     3061 gcgagcgcaa gaacggaacg cgccgcatgc cacgcgggct cgcgatcatc atttgctaca
     3121 tcatcttcat ctcggccgtc gtcggcttca tgttcctgct cgtgccgcgg ctctcgcgcg
     3181 acgtcgcgcg cctcggcaaa gaagcgcccg gtctctacaa gaaggtcaac gacgagtacg
     3241 tgccgtcgac ggcgcgttgg ctcgagcgga agttcccgtc cctcgccgaa gtgaagacgg
     3301 cgccggagca acaactgccg gtcgccgacg tgcccgagcc tcccggcacc gcattcacgg
     3361 tcacaccgtt gcccgacggc cggctcgcac tccagctcac gcccaacggc ctcgacatca
     3421 agccgcttcc ggacggcggc tatcacatca agacgctcga atttccgccc gagccggcaa
     3481 cgctcgaaga caagctccgc ggttacgtga agaagggcat cgtcgggctg cagagcaagc
     3541 tcaacgacgt cgtgcgcttc acgcaaacgc tgatcgccgg cttcatcaaa ggcatcttcc
     3601 tgttcttctt cacgctgatg atcggcgcgt tcatcctgat cgatctggaa aaagtccacg
     3661 cgttcatgcg cggcctcttc cccgcaaacg tgcgcgacga ctacgacgtc atcatcgcgg
     3721 gcatggaccg cggcctctcg ggcgtgatcc gcggccagct cctcatctgc ctcatcaacg
     3781 gcatcttcac gtacatcggc ctgctgatct tcaacgtgaa gtacgggttc atcctcgcga
     3841 ccgtcgcggg cctcatgagc ctcatcccga tcttcggatc gatcctgtca tccgtgccga
     3901 tcgtcacagt cgcgctcgca tccggcgaga aggggatcga tatcttccgc ggcgtcgcga
     3961 tgacgctctg gatcatcggc atccacttcg tcgaggcgaa cctcctgaac ccgaagatca
     4021 tcggctcggc cgcaaagatt catccggtcc tcgtgatctt cgcgctgttc ctcggcgaga
     4081 gcagcttcgg cctcgtcggc gcgttgctcg cagtgccaat tctttccgct atccaagtcg
     4141 tgttcatgta cttctatcga aaacgctgga aggacgcacc acgaccacaa acagggccga
     4201 tgccgagacc gccaggcgaa gcaggccctc tggcgccgac ccagcgctag tggtaacccc
     4261 gtaagcatgg tgcgaatgcg ggccgcgacg ctgctggcgc tttgcgtttc cagcgcagcg
     4321 tatgcccagg cacccgcacc cgatgcaccg ccgcctggct cgtcggaccc ggtgctcgat
     4381 gaacagatcg cgcagcagct cgtcgatcgc gcccaagagc ttctcgacgc gaagctctgg
     4441 ctcgacgcga aacagctcgc ggtcgaagcg cgcgtgaaga gcccgaaggg cccgtccgcc
     4501 gaacgcgctc gctacatcat caacatcgtc aaccagcacc tcgacatcaa agaggagccg
     4561 cctccgacgc cgccctccga ggtcccgccg acgacgacgc cacccaccgc aacgcccgac
     4621 atcccgcagg tcggcgatcg catcaatcca ttcgagccgc cggcaaaaga tccagaagag
     4681 ccgccacccg ccacaccgcc cggcaacggc aacccgcgca ccgccgcgat gatccacggc
     4741 ggcctctacg gcgccacgat cggcacgacg atcggggtcg cagcaaacag tgacaacccg
     4801 gccgccgccg caatcccact cggcatcgtc ggtggcgtcg gcgtcggcta cctctcgaca
     4861 aagctcgtcg cacgcgcgca ctgggacgaa gcccaagtcc ggacagccgg ctcctactcg
     4921 acgtggggca gcgccatcgg cggcctgttc ggcggcgcag ccacgggcgc cggtgacagc
     4981 agcccctcgg ccacgggcgt tctcgtcggc gcgagcatcg gctcgacgat cggcgccggc
     5041 gtcggcatcg cattcgcgcg cacgcacaag ttcacgcgcg gcgacgtcgc gctcaccgac
     5101 acgctcgcag gcatcggcgc ggtcggcggc ctcacgctcg gcatgctcat gcagccggtc
     5161 cagcccgaag cgtacgccgt caactcgatc gtcggcatct cggcgggcct cgtcgtcggt
     5221 ctcgtcgccg gtccacagac gaacacgacg cctcgccgca tgctgcgcgt cgccggcctc
     5281 tccgccgcag gtggcgccgc accgttcctc ctctacgcgg cgatctacga ctcgagctcc
     5341 gcatcggacg aacgcgtgac cggcctccta tcgagcgcag gtctcgtcgt cggcgcgtac
     5401 ctcggcttcc gcatcaccgc caagatggac gaaggcgtcg acaaggcaga cgcgccgccg
     5461 gatgacgcgc cgatcgcgct cgtcggtcgc agctcgaacg gcaactgggc gctcggtggc
     5521 atcggcgtgc agccgctctc gcgtcagctc gcgacgaaca atcgcggcat gacgttcacc
     5581 gtcgtcggcg cgaccttcta gctagagcaa cggctcggcg atcttgaacg ccttcgcctt
     5641 cacccagtcg gcggcggaga tgtcggcgga cgcgggatcg ctgtagccga gcatcgcctg
     5701 gaagtctgcc gcgagctcgc cgacggcggc gccgtcgcgc accgtaacga tcgactcgga
     5761 gttcgacgcc gcgctgcgcg gatcgaggtt gtacgagccg atcacggcga gcttgccgtc
     5821 gacgagcatc gtcttcgagt ggagcgtctc ggtgccgcgg tactgataga cctcgatgcc
     5881 cgcgccgagc agctctttgt agtagttcag cgacgcgtag tacatccacc aggcgtccgt
     5941 ggagtgcagc gagttcgtca cgacgacgac gcgcacgccg cgcgcgacag ccgccttgag
     6001 gtgcttgcgc agacgccgcg gcggcacgaa gtacgaattc gcgatgtaga tcgtgtgctg
     6061 cgcggtgcgc acgaggttca aggacgagag atagccaagc ggcgttttct ggctgcgagg
     6121 gtcgctatag accgggcgcg tgcgggcgct gcccgccgtc tc
//
LOCUS       DATLBZ010000368         2572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9371323, whole genome shotgun sequence.
ACCESSION   DATLBZ010000368 DATLBZ010000000
VERSION     DATLBZ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9371323"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1236)
                     /locus_tag="VMZ53_18795"
     CDS             complement(28..1236)
                     /locus_tag="VMZ53_18795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30569.1"
                     /translation="MTIVGTVLGKYRIESELSAGGMGEVYRAQHEILGRSAAVKVLRP
                     ELTENEGLVTRFINEAKAASAIRHPGIIEVLDFGTTDDGRQYLVMEFLDGESLAKRLE
                     KRTRLPDHEAAEIGRGIASALEAAHKKGIVHRDLKPDNVFLVPDIDLPSGERPKVLDF
                     GVAKLTDTNTRNTQTGALMGTPMYMAPEQARAAAAIDHRADLYSLGCILYELVVGNPP
                     FTGAGSGEIIALHMFEQPVPPRKHVMSVSPALEAIILKLLAKEPADRFQSAAQVAEAL
                     HLRNPAHSASLTIPHPIAAPPKRNLMPLVAGAITIAIAAVVVGVVMLQKSEAPKATPA
                     VTAPLPSPTSPPPPAPPVPVPVVDQPVQPPPAPPVVTKVKPPKTPKPPIATPPDNRHT
                     KNGFPTEVSP"
     gene            1272..1646
                     /locus_tag="VMZ53_18800"
     CDS             1272..1646
                     /locus_tag="VMZ53_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HUS30570.1"
                     /translation="MPSFQRHVFVCINERPADHPKGCCKAKGGVDVRDELKTELKKRG
                     ISKIVRANNSGCLDQCEHGVSVVVYPEQVWYGNVTVADIPEIVDKHLVGGEVVDRLLQ
                     PDQPHLATKRQFPPLDTSGAKK"
     gene            1643..2236
                     /locus_tag="VMZ53_18805"
     CDS             1643..2236
                     /locus_tag="VMZ53_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30571.1"
                     /translation="MKLLAVVATVASLSSVASADGTAEFDENADASAREANLESNARR
                     EGIMFGAALGGGVMLGGGVGRGPYVDFRLGHTATQRTQITFELVISSSLHKEAMAGKT
                     LTDSVAGLFVGAQRYTGVKSPVWFRGAGGLAFFQADFGKADPPKPLTGIGGLVGAGFD
                     LARWGKIVLGIESTAVGSLSSDGFKLDLNFGTNLIFY"
     gene            complement(2230..>2572)
                     /locus_tag="VMZ53_18810"
     CDS             complement(2230..>2572)
                     /locus_tag="VMZ53_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS30572.1"
                     /translation="WRSRTTQATVIVEPNATAYVTLDLGESKDKSVHGVVRDETGDAA
                     ANVAVTALIDEREASTVRTDKAGRFSITTRSGAQLLARGSGKIGHGIVGKANVPSEVV
                     DIVLDTSARDQ"
BASE COUNT          408 a          837 c          868 g          459 t
ORIGIN      
        1 ggagccgcca cctcatgccg ctctagttta cggcgaaacc tccgtcggaa agccgttctt
       61 ggtgtggcga ttgtctggtg gcgtcgcgat cggcggcttc ggggttttcg gaggcttgac
      121 cttcgtcaca accggcggcg ccggcggcgg ctgcaccggc tggtcgacga ctggcaccgg
      181 cacgggcgga gcaggcgggg gcggcgaggt tggagagggc agtggggcgg tgaccgccgg
      241 cgtcgccttt ggggcctccg acttttgcag catgacgacg cccacgacga ctgccgcgat
      301 cgcgatcgtg atcgcgccgg ccacgagcgg catgaggttg cgcttcggcg gcgccgcaat
      361 cggatgtgga atcgtcaggc tcgcgctgtg cgccgggttg cgcaggtgca gcgcctcggc
      421 gacttgcgcc gcgctctgaa agcgatcggc cggctccttc gcgagcagct tgagaatgat
      481 cgcctccagc gcgggcgaca cgctcatcac gtgctttcgc ggcggcacgg gctgctcgaa
      541 catgtgcagc gcgatgatct cgccggagcc tgcgccggtg aacggcgggt tcccgacgac
      601 gagctcgtac aagatgcagc ccagcgagta gaggtctgcc cgatgatcga tcgccgccgc
      661 cgcacgcgct tgttcgggcg ccatgtacat cggcgttccc atcagcgcgc cggtctgcgt
      721 gttgcgcgtg ttcgtgtcgg tgagcttggc gacgccgaaa tcgaggacct tcggacgctc
      781 gccactcggc aggtcgatgt ccggaacgag gaacacgttg tcgggcttca gatcgcgatg
      841 aacgatgccc ttcttgtgcg ctgcttcgag cgcgctcgcg atgccgcgcc cgatctcggc
      901 tgcttcgtga tccggcaagc gggtgcgctt ctcgaggcgc ttcgcgagcg attctccgtc
      961 gagaaactcc atcacgaggt actggcggcc gtcgtcggtc gtgccgaagt cgaggacctc
     1021 gatgatgccg gggtgccgga tcgcgctcgc agctttcgct tcgttgatga agcgcgtcac
     1081 gaggccctcg ttctcggtga gctccggccg gagcactttg accgcagccg agcgaccgag
     1141 gatctcgtgc tgcgcgcgat agacctcgcc cataccaccg gcggacagct cggactcgat
     1201 ccgatacttg cccaggaccg tcccgacgat ggtcacgccc cgagcatagc agtggtagcc
     1261 tgatcgggca gatgcccagt tttcagcgcc atgttttcgt ctgcatcaac gagcgtcccg
     1321 ccgatcatcc gaagggttgc tgcaaggcca agggcggagt cgacgttcgc gacgagctca
     1381 agaccgagct gaagaagcgc gggatctcca agatcgtccg cgccaacaac tcgggatgcc
     1441 tcgaccagtg cgagcacggc gtgagcgtcg tcgtctatcc ggaacaggtc tggtacggca
     1501 acgtgactgt cgccgacatc cccgagatcg tcgacaagca cctcgtcggc ggcgaagtcg
     1561 tcgatcggtt gctgcagccc gaccagccgc acctcgcgac gaagcgccag ttcccgccgc
     1621 tcgacaccag cggagccaag aagtgaagct cctcgcggtc gtcgcgacag tcgcctcgct
     1681 atcgagcgtc gcctctgccg acggcaccgc ggagttcgac gagaacgccg acgcgagcgc
     1741 gcgcgaggca aacctcgaat cgaacgcgcg tcgcgaaggc atcatgttcg gcgctgcgct
     1801 cggcggcggc gtcatgctcg gcggtggcgt cggtcgcgga ccgtacgtcg atttccggct
     1861 cggccacacc gcgactcagc gcacgcagat cacgttcgag ctcgtgatca gcagcagcct
     1921 tcacaaagaa gcgatggccg gcaagacgct caccgatagc gtcgcgggat tgttcgtcgg
     1981 tgcgcagcgc tacaccggtg tgaagagccc ggtgtggttt cgcggcgccg gcggcctcgc
     2041 gttttttcag gcggacttcg gcaaggcgga tccgccgaaa ccgttgacgg gcattggcgg
     2101 cctcgtcggc gcaggcttcg atctcgcgcg ctggggcaag atcgtcctcg ggatcgagtc
     2161 gaccgccgtc gggtcgctgt cgagcgatgg cttcaagctc gatctcaact tcggtacgaa
     2221 tctgatcttc tactgatcgc gcgcgctcgt atcgagaacg atgtcgacga cctcgctcgg
     2281 gacgttcgcc ttaccgacga tgccatggcc gatcttgccc gagccacgcg cgagcagctg
     2341 ggcacccgat cgcgtcgtga tcgagaagcg tcccgctttg tcggtgcgca ccgtcgaagc
     2401 ctcgcgctcg tcgatgagcg cggtgacggc gacattcgcg gcagcgtcac cggtctcgtc
     2461 gcgcacgact ccgtgcaccg acttgtcctt cgactcgccg agatcgagcg tgacgtaggc
     2521 ggtcgcattc ggctcgacga tgaccgtcgc ctgcgtcgtg cgcgagcgcc aa
//
LOCUS       DATLBZ010000369        13651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9372158, whole genome shotgun sequence.
ACCESSION   DATLBZ010000369 DATLBZ010000000
VERSION     DATLBZ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13651
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9372158"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VMZ53_18815"
     CDS             complement(<1..459)
                     /locus_tag="VMZ53_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HUS30573.1"
                     /translation="MSSSSSIESVLTENRSFPPSKEFSAASRVGSMADYEAMYRRADE
                     DPDGFWGEVAGELAWTKKWSRVLDWKLPDAKWFVGGELNASVSCLDRHVATWRKNKAA
                     LLWEGEPGDTRVLTYGQLHREVCKAANALASMGIKRGDFVAIYLPMIPEAV"
     gene            complement(469..861)
                     /locus_tag="VMZ53_18820"
     CDS             complement(469..861)
                     /locus_tag="VMZ53_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HUS30574.1"
                     /translation="MQRNIVRTAEAPAAIGPYSQAVVVPVGDQKMVFCSGQIALDPAT
                     GSMIEGDVAAQTRQVLANLGAVLAAADATFASVVKTTIFLASMDDFATVNAIYGERFV
                     HDPPARATVQAAKLPRGALVEIDAIAVI"
     gene            930..1004
                     /locus_tag="VMZ53_18825"
     tRNA            930..1004
                     /locus_tag="VMZ53_18825"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:962..964,aa:Val,seq:cac)
     gene            complement(1039..1965)
                     /locus_tag="VMZ53_18830"
     CDS             complement(1039..1965)
                     /locus_tag="VMZ53_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30575.1"
                     /translation="MRGSLLCAAIVCVAACGRLDFDALPDSRVTSDAAPDTPSRACPW
                     AANPTIGPVVRHTELSIARTETDPLLVRGDPLTIYFARDVSNGPQFDMFVAHRPTVDL
                     PFNAPQPVAELATVPLNEFAVQLDTTGHGYYVRSPNAGPQDIYELQKDAGGVLREVRA
                     LTELDTGGPEYDPFSSPDDKTIYFTAGSANGQDIFFATRASTSDPWSNVTPFAHNTNG
                     PDGGATLTRDGLVIVWAAEADVLVSSDIYYATRSSTSDPWGPKQLLGPGTISGSEHDL
                     EPGIREDGCELFFARSPSPTDGNWDIYSATLQ"
     gene            complement(2001..2534)
                     /locus_tag="VMZ53_18835"
     CDS             complement(2001..2534)
                     /locus_tag="VMZ53_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30576.1"
                     /translation="MRAAALVLLLLAACAGQTYSVSRITGTAKLRSDGAQGGEVSASF
                     EGHGVGLSLSAQAQQLRRSFPDGTQSRIGGGVDLGMRVSLFGLIGSSHEIDHWFDIGG
                     SAAGGGGFVVPARLTTYGEAWVGGWAAFGVYPGRRHLSIVLEVRRVAVTDWDNMTVFG
                     IGLSLTERFFEHFSIRD"
     gene            complement(2534..4270)
                     /locus_tag="VMZ53_18840"
     CDS             complement(2534..4270)
                     /locus_tag="VMZ53_18840"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HUS30577.1"
                     /translation="MKRFLIIASLLAFTTVADATPKTRSKAAKPAAAAVKAKTTTTRA
                     RVTFGKELRPAREVIGRREEPLTLEEQTAKQIEKLLRGPLRNGITGLFVADAATGEPL
                     FAVNADDPLNPASNVKMISTATALELLGPTFKYTTRLLANEPDASGTIKGDMFLLGTW
                     DPTLALSDMDKLAEQLAARGVHSLEGDVLVGGDPTRDGIYRAMIPLEIKAGEPTQPPT
                     VTPPANFDLFTFDITAKTDKRARKRHKLKVSTEIIDVDGKRRMKVTIAGTIGKGGETM
                     FPLYSRERTLVAAHALRAALKNHNITVTGDTRVMELGDFIGDSVKTHALPVEVARHES
                     PELQDIVRRINKFSINWLADRVIMTAAALSKRTQPSMDVAVEAMYGWLQRHPQLKKED
                     VTIDTGSGLSYRTRITPAELVKIVRAAGGYAPDAATAQDNAKAWLSSLSVAGTDGTLR
                     SRFRLSEVKGHVHGKTGSLSTVIALTGVLDLDPARPLAFSIVTNTQRPLKKGYVRKVH
                     EQLVGLIAEYVAATTKPTTTAPVSTPVQLGTPSVAEPPPLSEPTPDEETAAEAAEAQP
                     DPELDVETATSK"
     gene            complement(4267..4809)
                     /locus_tag="VMZ53_18845"
     CDS             complement(4267..4809)
                     /locus_tag="VMZ53_18845"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HUS30578.1"
                     /translation="MKGKSHGQRTKRPVLGPEEQSMFQEAMRDVTPLGSRDRVNVPPP
                     PPSVVKVVALPPEVKLTIEGDGQRYAARAAGVSHSQVAELRAGKIRAEATIDLHGSQV
                     EPAVRALEQFLVESRRLGRRCILVVHGKGLHSEHGAPLREGVLHALLGPLSGLVHALA
                     SASPTDGGEGATYVMLRGGR"
     gene            4940..6580
                     /gene="glgP"
                     /locus_tag="VMZ53_18850"
     CDS             4940..6580
                     /gene="glgP"
                     /locus_tag="VMZ53_18850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HUS30579.1"
                     /translation="MSPCPRVAYFCMEYGLDVSFPIYAGGLGILAGDHMKSVGDLKVP
                     LTGIGLFWDEGYTRQVIGPSGVEDHYPKTPRDALRRVDVGITVTVRGKEVPCRAWRVD
                     RHISSTPYLLEPERDDDRWITQRLYGGGVEDRLAQEILLGVGGVRLLQALGEKVDVYH
                     FNEGHAVFAGLELLRQNVYGGSSFEDRIAKLRPHVVFMTHTPVPAGNEVHDLELMRRM
                     DAALGFSDEELQKIGGAPFSMTVAGLRMSRLANGVAALHGETARDMWKEVGNAAPIIS
                     ITNGVHQPTWQDARIRAALVPEKTQAQQDKDLWTAHQAMKQELIDEVARVAGVQLALD
                     GLLIGFARRAAAYKRADLILGDERTLRRLFDFGIQLVYSGKAHPRDLAGKSLVGRLVE
                     ATKKYPGRVVFLENYDMHLGAVLTRGADIWLNNPRRPLEASGTSGMKAAMNGVPNLSI
                     LDGWWPEGCEHGVNGWKIGDPDPTDDPFDEKDEARVDRRDREILHNVLEQEVLPAYQE
                     RSRWIALMRASIAMSQWRFSSDRMIEDYVSQVYSSSGR"
     gene            complement(6559..7488)
                     /locus_tag="VMZ53_18855"
     CDS             complement(6559..7488)
                     /locus_tag="VMZ53_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30580.1"
                     /translation="MTRAAVLALAVLIVPACQPSGTSTSRPTPAQQYAVLDLVGDWRW
                     LYRTDAQGTTRVEEENWRLAPDPESPTHLVGRYLRSVEVRSTDVLPFTCNQRRQYKQR
                     ALYDVTVDLENDGLRIRETGYDAEDSPCDHGFRHTSEYTADARGNRLALRWPDGTQTL
                     WHVDGETRALPEAPWAQPHEPTGAWRWQVVSYDDDGNLRSEAEWWEISRRTDTQLDAT
                     YRRRVTVRSPDGKPIACAGASSWSFDDAYVLRGQREEEHWHFFELAVDAGTHPCISDK
                     RALDEATAEQIGSYFVLEWRGKRRQVLYRPDDE"
     gene            complement(7485..7760)
                     /locus_tag="VMZ53_18860"
     CDS             complement(7485..7760)
                     /locus_tag="VMZ53_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30581.1"
                     /translation="MSFEWLDCTPGKNNGSELRSDVAAKEIADRAGSMFRLGFSQAEA
                     TKRLCERIAWEFDPPSKHSCHKRPDALSDKAIEKIVADTYARRPGGW"
     gene            7782..9059
                     /locus_tag="VMZ53_18865"
     CDS             7782..9059
                     /locus_tag="VMZ53_18865"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS30582.1"
                     /translation="MRLLSIAGAAIAAFGLGAWDTPAARADGTGVIAVTTSPEATDAT
                     AVRTSIATALVQSRTSPGATTPPRIAEDAVAEARVALAEGAIPIATLERFRRLREQID
                     EAWRAYLRVQVDFAASRLAAARTDAESLVAIPGGALIYADASLRLGAVLAHLGRTNDS
                     QAALALALALDPDRPITLAEFSPDVVAAVDAVRAQKKPTRTVRITTDPAGSALTIDGR
                     DAGHAPINLDLAVGQHVIVARQPLHQARALAIAVDDAPTSAMQTIDLALERDIAAARL
                     AEGAQPGLPDAASQELADAAVRYADLDEVVLVAATDRRGGPALLVQRCAGLPARCTAV
                     VEVGYGNRAGLAAAAREAWSDVATADLRYPPSVFNDPRVTGKKLDDRCKVCRSPYLWG
                     GVGLAAVVTTIIVVAVVSSSQPPPVLGADPTKF"
     gene            complement(9081..11405)
                     /locus_tag="VMZ53_18870"
     CDS             complement(9081..11405)
                     /locus_tag="VMZ53_18870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30583.1"
                     /translation="MTELPTSFGKYYLAEKLATGGMAEIYLAKIIGPGGFEKPVVIKQ
                     IHPKLTGQRHFVDLFVNEAKTLVTLSHGNIVPVYELGVIEDTYFIAMDYIDGPTLYRL
                     TETMARREATMEPAVAAWITARMLEGLDYAHRKGEGVIHRDLSPRNVMLSRDGDVKLV
                     DFGIAVTLGDPGEGTARESAPTGSFPYMSPEQVRREPLTGQTDLFSAGVLLWEMLTGQ
                     RLFARPDADATLDAVLRDEIPRPSSVRPEVPAKLDDVVMRALERDRGARWALAADMLA
                     ALNKYLYSLDETPGPRDVSALVARFCPPETRRLPTHLEAIAHDVLSPEPFGGGRPPTE
                     APLASGPSTAVIPRDQQARGKPLRQKSFATNVNLMAILEQADPLPRFEEKSDLLPSFD
                     EKSDVLPAFHEAADNPTKDDRPKSPSRVDTPLDKPSARSPFAADTVIDDAPPVLPHAP
                     VTKPPRAPTQQPGSRAPTQPPATKRRSRPMDTVDETPEEAIERPIRRTPRSVSSQHGI
                     AHVERAANPDDRGHVERARGQTDRRSGSIRARSYPDAGDVERARNEAKRARSRAEPGD
                     RDTEPAYSKERTAVDGSPLGENVAKSFRQPPSRSFLVLAGFGALALAGAAVFVFFQGR
                     DHVLAMTEREAGVPPADGAVADVPTLSDAAPVVDDATLQPTATADADIALTRDAGTKH
                     GDAPIRTSIDATPQTVDAAVAKGSGTLQVGADPWGEIYVDGKSMGRTPKELTVTAGHH
                     TVEVVFAGETPPRKQTFAVDISNGETKPIQADFK"
     gene            11441..11995
                     /locus_tag="VMZ53_18875"
     CDS             11441..11995
                     /locus_tag="VMZ53_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS30584.1"
                     /translation="MRRWIALLVSSGCAQIGGLDKTTAPADASGDSRVIDTAYFDAKP
                     CSGGDAAMLDPTYGHCYVYYATPKDYLAARTTCTSMTGFHLARVETSNEQPLIASLVG
                     ATALAYIGGNDIAAEGTYVWDDGTLIQLTSWNTGEPNNGAAMFEEDCIVVNGPTGGHW
                     DDRPCSPAISATVPGEYGYVCERD"
     gene            12013..12555
                     /locus_tag="VMZ53_18880"
     CDS             12013..12555
                     /locus_tag="VMZ53_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS30585.1"
                     /translation="MRWWIPLVLLIGCGGDDSSSHVDAGHGDPDGRRVDAGPCTGGQV
                     AMTDPMTGHCYLLFTAPKAWAVAKTSCDAMAGFHLVRISSASEQMVAKAVSGLDVSWI
                     GGSDSATEGTFVWDDGTATSYTNWKSGEPDNGANEVGAVEEDCIALDGGFSGGWTDRP
                     CAPTSGTSVPSTYPYICERD"
     gene            12540..>13651
                     /locus_tag="VMZ53_18885"
     CDS             12540..>13651
                     /locus_tag="VMZ53_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30586.1"
                     /translation="MRARLALVLLAACGEPAAHVQLAPVPLPGGCGLTTNKTSLRVIA
                     FTPGGQLRRTVPPTEIDAFPADTEQLGVEVVGGSTGLLAIGKTAVLEYGELAEGTQIP
                     ILMVPPNGFCPVGALGEPRVAPLVARAGEGVLVAGGTGASGEPLSSAEYFDPRAATFS
                     AIEVPSLLVDAANGLAGAVLTELPDGRVALSGTASHALTIFDPATRTFSTPTLFDHRA
                     YHGAVAPDDEHLFVIGGCADVVSGACATPYLRTGFSYELANVANRTRGPTLDDNAARV
                     GAHIERIGLGASGEDEYVLVGGSGDADVADRFTIAGDSATTITGLHAQTVVLDGGALF
                     SALDADGTGQTGAASMAAPTATSATAMQLAPRLDGAR"
BASE COUNT         2132 a         4491 c         4691 g         2337 t
ORIGIN      
        1 cacggcctcc ggaatcatcg gcaagtagat cgcgacgaag tcgccgcgct tgatgcccat
       61 gctggcgagc gcgttcgccg ccttgcagac ctcgcggtgc agctgcccgt acgtcagcac
      121 gcgcgtgtcg ccgggctcgc cctcccagag cagcgccgcc ttgttcttgc gccacgtcgc
      181 aacgtgccga tccaaacagc tcacgctcgc gttgagctca ccaccgacga accacttcgc
      241 gtcggggagc ttccagtcga gcacgcggct ccacttcttc gtccacgcca gctcgcccgc
      301 tacctcgccc cagaacccat cgggatcctc gtcggcgcgc cgatacatcg cctcgtagtc
      361 ggccatgctg ccaacgcgcg atgcggccga aaactctttc gacggcggga agctacggtt
      421 ctcggtcagt accgactcga tcgacgacga agacgacaca gtggcctcct agatgacggc
      481 gatggcatcg atctcgacga gcgctccacg cgggagcttc gccgcttgca ccgttgcacg
      541 cgccggcggg tcgtgcacga agcgctcgcc gtagatcgca ttcacggtcg cgaagtcgtc
      601 catgctcgcg aggaagatcg tcgtcttcac gacgctggca aacgtcgcat ccgccgccgc
      661 caacacagcg cccaggtttg cgagcacctg ccgggtctgc gccgcgacat cgccctcgat
      721 catcgagccc gtcgccggat cgagcgcgat ctgcccgctg caaaacacca tcttctggtc
      781 gccgaccgga acaaccacgg cctgcgaata cggcccgatc gccgcgggag cctcggcggt
      841 tctcacgatg ttgcgctgca tgccgccgtc gtatcacatg acgcgattga caggccttag
      901 gcagggttgc tacacaacgg tcctctcacg ggcgtttaac tcagcgggag agtgattcct
      961 tcacacggaa taagtcgggg gttcaatccc ctcaacgccc accacggcca ccggaaacgg
     1021 tggtcgtttt cgtttcggct actgcagcgt cgccgagtag atgtcccagt tgccgtcggt
     1081 cggcgacggg ctgcgcgcga agaacagctc gcaaccgtct tcgcgaatgc ccggctcgag
     1141 gtcgtgctcg gatccggaga tcgtgccggg gccgaggagc tgcttcggtc cccatggatc
     1201 gctcgtgctc gaacgcgtcg cgtagtagat gtcgctcgac acgaggacgt cggcctcagc
     1261 ggcccacacg atcacgaggc cgtcgcgggt gagcgttgcg ccgccgtcgg ggccattcgt
     1321 gttgtgcgcg aacggagtga cgttggacca cggatcgctc gtcgacgcgc gcgtggcgaa
     1381 gaagatgtcc tggccgtttg cgctccccgc ggtgaagtag atcgtcttgt cgtcagggct
     1441 cgagaatgga tcgtattcgg ggccacccgt gtcgagctcg gtcagcgcgc gcacttcgcg
     1501 gagcacaccg cccgcgtctt tctggagctc gtagatgtct tgcggacccg cgtttggact
     1561 acgcacgtaa tagccgtggc cagtggtgtc gagctgcacg gcgaactcgt tgagcgggac
     1621 tgttgcgagc tcggcgacgg gctgcggcgc gttgaagggg aggtcgaccg tcgggcggtg
     1681 cgcgacgaac atgtcgaact gcgggccgtt cgagacatcg cgtgcgaagt agatcgtcag
     1741 tgggtcgccg cgcacgagga gcggatcggt ttcggtgcgc gcgatcgaga gctctgtgtg
     1801 acggacgacg ggtccgatcg tcgggttcgc tgcccacgga cacgcacgcg acggtgtgtc
     1861 gggcgcggcg tcggaagtga cgcgcgagtc gggtagtgcg tcgaagtcga ggcgcccgca
     1921 cgccgcgacg cacacgatcg ccgcgcacaa caacgagccc cgcacggtcg catcttatcg
     1981 cgcgcgtgaa tcgcggcgtg ttagtcgcga atgctgaagt gttcgaagaa gcgttcggtc
     2041 agcgagagac cgatcccgaa taccgtcatg ttgtcccagt ccgtcactgc gacgcgccgc
     2101 acctcgagga cgatcgagag atggcgacgc cccgggtaga cgccgaatgc agcccagccg
     2161 ccgacccagg cttcgccgta cgtcgtcaga cgcgccggaa cgacgaagcc accgccgcct
     2221 gcagcggagc cgccgatgtc gaaccaatga tcgatctcgt gcgagctgcc gattaggccg
     2281 aacagcgaga cgcgcatgcc gaggtcgacg ccaccgccga tgcgcgattg cgttccgtcg
     2341 gggaatgagc gccgcagttg ctgcgcttgc gcggagagcg agagcccgac gccgtggcct
     2401 tcgaacgatg cggagacttc gccgccctgc gcgccgtcgc tgcggagctt cgcggtcccg
     2461 gtgattcgag acacggaata ggtctggcct gcacatgcgg cgagaaggag gaggacgagc
     2521 gcagccgcgc gcattacttc gacgtggcgg tctcgacgtc gagctcggga tcgggctgcg
     2581 cttcagccgc ttcggcggcg gtttcttcgt cgggcgtcgg ctcgctgagc ggcggcggtt
     2641 cggcgacgga cggcgtgccg agctggacgg gcgtcgagac gggcgccgtc gtcgtcggct
     2701 tggtcgttgc ggcgacgtac tcggcgatga ggccgacgag ctgctcgtgc actttgcgga
     2761 cgtagccctt cttgagcggg cgctgcgtgt tcgtgacgat cgagaacgcg agggggcgtg
     2821 cggggtcgag gtcgagcacg ccggtcagtg cgatcacggt ggagagcgag ccggtcttgc
     2881 cgtggacgtg gcctttgacc tcggagagac ggaagcggct gcgcagcgtg ccgtcggtgc
     2941 cggcgacgga cagagagctc agccacgcct tcgcgttgtc ttgtgcggtg gcggcgtcgg
     3001 gtgcgtagcc gcccgcggcg cgcacgatct tcacgagctc tgctggagtg atgcgcgtgc
     3061 ggtaagagag gcccgagccg gtgtcgatcg tgacgtcttc cttcttgagc tggggatgac
     3121 gctgcagcca gccgtacatc gcctcgacgg cgacatccat cgacggttgc gtccgcttcg
     3181 acagcgcggc ggccgtcatg atcacgcggt cggcgagcca gttgatcgag aacttgttga
     3241 tgcggcggac gatgtcttgc agctcgggcg actcgtggcg cgcgacctcg acgggcaggg
     3301 cgtgcgtctt gaccgagtcg ccgatgaaat cgccgagctc catcacgcgc gtgtcgccgg
     3361 tgaccgtgat gttgtggttc ttgagcgctg cgcgcagagc gtgtgcagcg acgagcgtgc
     3421 gctcgcgcga gtagagcggg aacatcgtct cgccgccctt gccgatcgtc cccgcgatcg
     3481 tgaccttcat gcggcgcttg ccgtcgacgt cgatgatctc cgtcgagact ttgagcttgt
     3541 gacgcttgcg agcgcgcttg tcggtcttcg ccgtgatgtc gaacgtgaag agatcgaagt
     3601 ttgcgggcgg cgtgactgtc ggcggctgcg tcggttcgcc ggctttgatc tcgagcggga
     3661 tcatcgcgcg gtagatgccg tcgcgagtcg ggtcaccacc gacgagcacg tcgccttcga
     3721 gcgagtgcac accgcgcgcc gcgagttgct cggcgagctt gtccatgtcc gagagcgcga
     3781 gggtcggatc ccacgtaccg aggaggaaca tgtcgccctt gatcgtgccg ctcgcgtcgg
     3841 gctcgttggc gagcaagcgc gtcgtgtact tgaacgtcgg gccgaggagc tcgagcgcgg
     3901 tcgccgtcga gatcatcttg acgttcgacg ctgggttcag cggatcgtcg gcattgaccg
     3961 cgaacagagg ctcaccggtc gcggcgtctg cgacgaacag gcccgtgatg ccgttgcgaa
     4021 gcggaccgcg cagcagcttt tcgatttgct ttgcggtctg ctcttcgagc gtgagcggct
     4081 cttcgcgccg gccgatgact tcgcgtgcag gacgcagctc tttgccgaag gtgacgcgag
     4141 cgcgcgtcgt cgtcgtcttc gctttgactg cggcggctgc gggcttcgcg gccttcgagc
     4201 gcgtcttggg agtggcatcc gcgaccgtcg tgaatgcgag cagcgaggcg atgatgagga
     4261 accgcttcat cggccacctc gcagcatgac gtacgtcgcg ccttctccgc cgtcggtggg
     4321 cgatgcggag gcgagcgcgt ggacgaggcc ggagagcggg ccgagcagcg catggagcac
     4381 gccttcgcgc agcggcgcac cgtgctcgga gtgcaggcct ttgccgtgga ccacgaggat
     4441 gcaacgccgg ccgagacgtc gcgattcaac gaggaactgc tcgagtgcgc gcaccgctgg
     4501 ttcgacctgc gagccgtgca gatcgatggt cgcctccgcg cgaatctttc cggcgcgaag
     4561 ctcggcgacc tgcgagtgcg aaacgccggc ggcgcgcgcg gcatagcgct gaccatcgcc
     4621 ctcgatcgtc agcttcactt cgggcggaag ggcgaccacc ttcacgacag acggcggcgg
     4681 cggcggaacg ttcacgcgat cgcgactgcc gagcggggtg acgtcgcgca tggcctcctg
     4741 gaacatcgac tgttcctcgg gacccagcac cggccgctta gtgcgctggc cgtgcgactt
     4801 acccttcatc cctccattgt gtactcgccg tgcgctccaa ccgccaagaa atgttgatta
     4861 acgcaacgcc gcccatccct ggcactttcg ggcgcaccgg cttgcggacg tgacggtcgt
     4921 caaggcactt tcctggccga tgtcgccgtg tccccgtgtc gcgtacttct gcatggagta
     4981 cgggctcgat gtgtcgttcc ccatctacgc aggcggcctc ggcattctcg ccggtgacca
     5041 catgaaatcg gtcggcgact tgaaggtccc gctgaccgga atcggcctgt tctgggacga
     5101 ggggtacacg cgccaggtga tcggcccatc gggggtcgag gaccactatc ccaagacccc
     5161 acgcgacgca ctgcgccgag tggacgtcgg gatcaccgtc accgtccgcg gcaaagaagt
     5221 gccgtgccgg gcctggcggg tcgatcgtca catctcgtcg acgccctacc tgctcgagcc
     5281 cgagcgcgac gacgataggt ggatcacgca gaggctctat ggcggcggcg tcgaagaccg
     5341 cctggctcag gagatcttgc tcggcgtcgg cggcgtccgg ttgctgcaag cgctcggcga
     5401 gaaggtcgac gtctatcact tcaacgaggg gcacgcggtg ttcgcgggtc tcgagctgct
     5461 gcggcagaac gtctacggcg gttcgtcgtt cgaggatcgg atcgcgaagc tgcgcccgca
     5521 tgtcgtgttc atgacgcaca cgcccgttcc ggccggcaac gaggtgcacg acctcgagct
     5581 catgcgccgc atggacgccg cgctcggctt ctccgacgag gagctgcaga agatcggcgg
     5641 cgctccgttc tcgatgacgg tggcaggcct gcggatgtcg cgactcgcaa acggcgtggc
     5701 ggcgctccac ggcgagaccg cgcgcgatat gtggaaagag gtcggcaacg cggcgccgat
     5761 catcagcatc acgaacggcg tccatcagcc gacctggcag gacgcgcgca ttcgcgccgc
     5821 cctcgtgccc gagaagacgc aggcgcagca ggacaaggat ctgtggactg cgcaccaggc
     5881 gatgaagcag gagctcatcg acgaggtcgc tcgcgtcgcg ggtgtgcagc tcgcgctcga
     5941 cggtttgctt atcggctttg cgcgtcgcgc cgctgcctac aaacgcgccg acctcatcct
     6001 cggtgacgag cgcaccctgc gccggctgtt cgatttcggt atccagctcg tctactcggg
     6061 caaggcgcac ccgcgcgacc tcgcgggcaa gtcgctcgtc ggccggctcg tcgaagcgac
     6121 gaagaagtat cccggccgcg tcgtgttcct cgagaactac gacatgcacc tgggcgcggt
     6181 gctcacgcgc ggtgccgaca tctggctcaa caacccgcgc cgcccgctcg aagcgtcggg
     6241 cacgagcggc atgaaggccg cgatgaacgg tgtgccgaac ttgtcgatcc tcgacggctg
     6301 gtggccggaa ggctgcgagc acggcgtcaa cggctggaag atcggcgacc ccgatccgac
     6361 ggacgatcca ttcgacgaga aggatgaagc gcgcgtcgat cggcgtgatc gcgagatcct
     6421 gcacaacgtg ctcgagcaag aggtgctccc cgcgtatcag gagcgctcgc gttggatcgc
     6481 gctcatgcgc gcgtcgatcg cgatgtcgca gtggcggttc tcgtccgatc gcatgatcga
     6541 ggactacgtg tcgcaggtct attcgtcgtc gggacgatag agaacctggc ggcgcttgcc
     6601 gcgccactcg agcacgaagt agctcccgat ttgctcggcg gtcgcttcgt ccagtgcgcg
     6661 cttgtccgag atgcacgggt gcgtgcccgc atcgacggcg agctcgaaga agtgccaatg
     6721 ctcctcttcg cgctggccgc gcaagacgta cgcatcgtcg aagctccacg acgaggcgcc
     6781 ggcgcacgcg atgggcttgc cgtccggaga tcggacagtt acccggcggc ggtacgtcgc
     6841 gtcgagctgc gtgtccgtgc ggcgcgagat ttcccaccac tcagcctcgg agcgcaagtt
     6901 gccgtcgtcg tcgtacgaga cgacttgcca gcgccacgcg ccggtcggct cgtgcggctg
     6961 cgcccatggc gcttcgggca acgcgcgggt ctcgccgtcg acgtgccaca gcgtctgcgt
     7021 gccgtcgggc cagcgcagtg cgagacgatt gccgcgtgcg tccgcggtgt actcgctcgt
     7081 gtggcgaaat ccgtggtcgc acggcgagtc ctcggcgtcg tagccggtct cgcgaatccg
     7141 caggccatcg ttctcgaggt cgacggtgac gtcgtacaga gcgcgctgct tgtactgccg
     7201 ccgctggttg cacgtgaatg gcagcacgtc ggtcgagcga acctcgacgc tgcgcaggta
     7261 gcgaccaacg agatgcgtcg gcgattcggg atccggcgcg agccgccagt tctcttcctc
     7321 gacgcgcgtc gtgccctggg cgtcggtgcg atacagccag cgccaatcgc cgacgagatc
     7381 gagcaccgcg tattgctgcg caggtgtcgg cctcgacgtg ctcgtaccgg acggctggca
     7441 tgccggcaca atgaggaccg cgagagcgag gaccgccgcg cgtgtcacca gccgccggga
     7501 cggcgcgcgt acgtatcggc gacgatcttc tcgatcgcct tgtccgacag cgcgtcggga
     7561 cgcttgtggc agctgtgctt cgacggcggg tcgaactccc acgcgatacg ctcgcacagc
     7621 cgcttcgtcg cttcggcctg agaaaagccg aggcggaaca tcgagccggc ccgatccgcg
     7681 atctccttcg ccgccacatc cgagcgaagc tcggagccgt tgttctttcc aggcgtgcag
     7741 tcgagccatt cgaaggacat gtggcgagta tactcatccg cgtgagactt ctgtcgatcg
     7801 cgggtgcagc gatcgcggcg ttcgggctgg gcgcatggga cacgccggcc gcgcgcgccg
     7861 acggcacggg tgtgatcgcg gtgacgacgt cgcccgaagc gaccgacgcg acagcagtcc
     7921 gcacatcgat cgcgacggcg ctcgtccaat cgcggacctc acccggcgcg accacgccac
     7981 ctcgcatcgc cgaagacgcc gtcgccgaag cacgcgtcgc actcgccgaa ggcgcgatcc
     8041 ccatcgcaac gctcgagcgc ttccgccggt tgcgcgagca gatcgacgag gcatggcgcg
     8101 cctacctccg cgtgcaagtc gacttcgccg cgtctcgcct cgctgccgct cgcaccgacg
     8161 ccgaatcgct cgtcgccatc ccaggcggtg ccctcatcta cgccgatgcc tcgctgcgcc
     8221 tcggcgccgt gctcgcgcat ctcggccgca cgaacgactc gcaagctgcg ctcgcgcttg
     8281 cgctggcgct cgatcccgat cgcccgatca cgctcgccga gttctcaccc gacgtggtcg
     8341 ccgccgtcga cgccgtgcgc gctcagaaga agccgacgcg cacggtgcgc atcacgaccg
     8401 accccgcggg ctcggccctc acgatcgacg gccgcgatgc gggtcacgcg ccgatcaatc
     8461 tcgacctcgc agtcggccag cacgtgatcg tcgcacgtca gccactccac caagcacgcg
     8521 cgctcgcgat cgcggtcgac gatgcgccca cctccgccat gcagacgatc gacctcgcgc
     8581 tcgaacgcga catcgctgcg gcacgtctcg ccgaaggcgc gcagcccggc cttcccgatg
     8641 cagcctcgca agagcttgcc gacgcggcgg tgcgctacgc ggatctcgac gaagtcgtcc
     8701 tcgtcgccgc cacggatcgc cgcggtggtc ccgcgctgct cgtccaacgc tgcgcaggcc
     8761 tccccgcacg ctgcaccgct gtcgttgaag tcggctacgg caatcgcgcc ggtctcgcag
     8821 ccgctgcgcg cgaagcatgg agtgatgtcg ccaccgccga ccttcgctac ccgcccagcg
     8881 tcttcaatga tccgcgcgtg acgggcaaga agctcgacga tcgttgcaag gtctgccgca
     8941 gcccatacct ctggggtggc gtcggcctcg ccgcagtcgt cacgacgatc atcgtcgtcg
     9001 cggtcgtctc gtcgtcacaa ccaccgcccg tcctcggcgc cgatccgacc aagttctaga
     9061 aacagcgtcg cgagcgacgt ttacttgaag tcggcttgga tcggcttggt ctcgccgttc
     9121 gagatgtcga cggcgaacgt ttgcttgcgt ggcggcgttt caccggcgaa cacgacctcg
     9181 acggtgtgat gcccggccgt cacggtgagc tccttcggcg ttcggcccat cgatttgccg
     9241 tcgacgtaga tttcgcccca tgggtcggcg ccgacctgta gcgtgccgct gcctttcgcg
     9301 accgcggcat cgaccgtctg tggtgtggca tcgatgctcg tgcgaatcgg cgcatcaccg
     9361 tgcttggtgc ctgcgtcgcg cgtgagcgcg atgtcggcgt cggcggttgc ggtgggctgc
     9421 aacgtcgcat catcgaccac cggcgctgcg tcggacagcg tgggcacgtc agcgacggcg
     9481 ccatcggccg gcgggacacc cgcttcgcgc tcggtcatcg cgagcacgtg atcgcggcct
     9541 tggaagaaca cgaacacggc ggcacccgcg agtgcgagcg cgccgaagcc cgcgagcacg
     9601 aggaagctcc ggctcggcgg ctgccgaaac gatttggcga cgttctcgcc gagcgggctg
     9661 ccgtcgacgg cggtgcgctc tttcgagtac gcgggctcgg tgtcgcggtc accgggctcg
     9721 gcccggctgc gcgcgcgctt cgcctcgttg cgtgcacgtt cgacgtcacc ggcatcggga
     9781 tagctgcgag cccgtatgga accgctacga cgatcggtct gaccgcgcgc acgttcgaca
     9841 tgcccacgat cgtcgggatt ggctgcacgt tcgacatgcg cgatgccgtg ctgcgaggag
     9901 acgctgcgtg gcgtgcggcg gatcggccgc tcgatcgcct cttcgggcgt ctcgtcgacg
     9961 gtgtccatcg gccgcgaacg tcgcttcgtc gcgggtggct gcgtcggggc gcgcgagccg
    10021 ggctgctgcg tcggggcgcg tggtggcttg gtgacgggcg cgtggggaag cacgggcggc
    10081 gcgtcgtcga tcaccgtgtc cgcggcgaac gggctcctcg cagatggctt gtcgagcggc
    10141 gtgtcgacgc gcgatggcga cttcggtcga tcgtccttcg tcgggttgtc ggccgcttcg
    10201 tggaacgccg gcagcacgtc ggacttctcg tcgaagctcg gcagcagatc cgatttctcc
    10261 tcgaagcggg gcagagggtc cgcttgctcg aggatcgcca tcagattcac gttcgtcgcg
    10321 aacgacttct ggcgtagcgg cttgccgcgc gcttgttggt cgcgtgggat aaccgcagtc
    10381 gacggtccac tcgcgagcgg agcttcggtc ggtgggcggc cgccgccgaa tggctcgggc
    10441 gacaggacgt cgtgcgcgat cgcttcgagg tgcgtcggca agcgccgggt ttccggcggg
    10501 cagaagcgag cgacgagcgc ggagacgtcg cgcgggccgg gcgtctcgtc gagcgagtag
    10561 agatacttgt tcagcgcggc gagcatgtcg gcggcgagcg cccaccgcgc accgcgatct
    10621 cgctcgagcg cacgcatcac gacgtcgtcg agcttggccg gaacttcggg tctcacagac
    10681 gacgggcgcg gaatctcgtc gcgcagcact gcatcgagcg tggcgtcggc atctggacgc
    10741 gcgaacaggc gctggccggt gagcatctcc cacaggagca cgcccgccga aaacagatcg
    10801 gtctgcccgg tgagcggctc gcggcgaact tgctcgggcg acatgtacgg gaacgagccg
    10861 gtcggcgccg attcacgagc ggtcccttcg cccggatcgc cgagcgtcac cgcgatgccg
    10921 aagtcgacga gcttgacgtc gccatcgcgc gacagcatca cgttgcgcgg cgagaggtcg
    10981 cgatggatca cgccttcgcc tttgcgatgc gcgtagtcga gaccttcgag catgcgtgcc
    11041 gtgatccacg cggccacggc cggttccatc gtcgcttcgc ggcgagccat cgtctcggtg
    11101 agccggtaga gcgtcggccc gtcgatgtag tccatcgcga tgaagtaggt gtcttcaatg
    11161 acgccgagct cgtagaccgg aacgatgttg ccgtgcgaga gcgtgacgag cgtcttcgct
    11221 tcgttgacga acaaatcgac gaagtgccgc tgcccggtca gcttcggatg aatctgcttg
    11281 atgacgacgg gcttctcgaa gccgccgggc ccgatgatct tggcgagata gatctcggcc
    11341 attccgccgg tcgccagctt ctcggccagg tagtacttgc cgaacgaggt cggcaactcg
    11401 gtcatcggat acgagcgtat cagaggtatg cttggcccgg atgcgacggt ggatcgcgct
    11461 cttggtctcg agcggctgcg cgcagatcgg cgggctcgac aagacaacag ctcccgctga
    11521 cgcgtcggga gatagccgcg tgatcgatac ggcgtacttc gacgcgaagc cgtgctccgg
    11581 cggggatgcg gcgatgctcg atccgacgta cggccactgc tacgtgtact acgcgacgcc
    11641 aaaggactac ttggccgcac gcacgacctg cacgtcgatg acggggttcc acctagcgcg
    11701 cgtcgagacg tcgaacgagc agccactgat cgccagcttg gtcggcgcaa ccgcgctcgc
    11761 gtacatcggc ggcaatgaca tcgctgccga aggcacgtac gtctgggatg acggcacgct
    11821 gattcagctg acgagctgga acacgggtga gccgaacaac ggcgccgcga tgttcgagga
    11881 agactgcatc gtcgtgaacg ggcccaccgg tgggcattgg gacgatcgcc cgtgctcgcc
    11941 cgcgatctcg gcgacggtgc ccggcgagta cggctacgta tgcgagcgcg actaagaggt
    12001 atgctcgccg agatgcgttg gtggattccg ctcgtgctct tgatcggctg cggtggcgac
    12061 gattcgtcct cgcatgtaga tgccggtcat ggagaccccg atggtcgacg cgtcgacgcc
    12121 ggaccgtgta cgggcggtca agtcgcgatg accgatccta tgacggggca ctgctacctg
    12181 ctgttcaccg cgcccaaggc gtgggcggtc gcgaagacga gctgtgatgc gatggcgggc
    12241 tttcacctcg tgcgcatctc gtcggcgagc gagcagatgg tcgccaaagc ggtgtcgggt
    12301 ctcgatgtct cgtggatcgg cggcagcgac agcgcgacgg aaggcacgtt cgtctgggac
    12361 gacggcaccg ccacctcgta cacgaactgg aagtccggcg agccggacaa cggcgcgaac
    12421 gaggtcggcg ctgtcgaaga agactgcatc gcgctcgacg gcggcttcag cggtgggtgg
    12481 accgatcgcc cgtgcgcacc gacgagcggc acgtccgtac cgagcacgta cccatacata
    12541 tgcgagcgcg actagcactc gtcctgctcg ctgcgtgcgg cgagcccgct gcgcacgtgc
    12601 agctcgcacc ggtgccgctg ccgggtggat gcggcctgac gacgaacaag acgagcttgc
    12661 gcgtcatcgc gtttacaccg ggcggccagc tgcgtcggac ggtgccaccg acggagatcg
    12721 atgcatttcc cgcggacacc gagcagctcg gcgtcgaagt cgtcggcggt tcgacgggcc
    12781 tgctcgcgat cggcaagacg gccgtgctcg agtacggaga gctcgctgaa ggcacccaga
    12841 ttccgatcct catggtgccg ccgaacggat tttgccccgt cggtgcgctc ggcgagccac
    12901 gtgtcgcgcc gctcgtcgcg agggcaggcg agggcgtgct cgtcgcggga ggtaccggcg
    12961 cgagcggcga gccgctgtcg agtgcggagt acttcgatcc acgcgcggcg acgttctcgg
    13021 cgatcgaagt gccatcgctc cttgtcgatg ccgcgaacgg cctcgcgggt gccgtgctca
    13081 ccgagctgcc cgatggccgc gtcgcgctca gcggcacggc gagccacgcg ctgacgatct
    13141 tcgatccggc gacgcgcacg ttctcgacgc caacgttgtt cgatcatcgc gcgtatcacg
    13201 gcgcagtcgc gccggacgac gagcatctct tcgtcatcgg cggttgtgcg gacgtcgtga
    13261 gcggcgcgtg cgcgactccg tatctgcgaa ccggcttttc gtacgagctc gcgaacgtcg
    13321 cgaaccgcac ccgcggccca acgctcgacg acaacgcagc ccgcgtgggc gcgcatatcg
    13381 aacgcatcgg cctcggtgca tccggcgagg acgagtacgt gctcgttggt ggcagcggtg
    13441 atgcagatgt cgccgatcgg ttcacgatcg ccggtgactc tgccacgacg atcactggcc
    13501 tccacgcgca gacggtggtg ctcgacggtg gcgcgctgtt cagcgcactc gacgccgacg
    13561 gcaccgggca gacgggagcc gcgtcgatgg ccgccccgac agcaacctcc gcgacagcga
    13621 tgcagctcgc tcctcggctc gatggcgcgc g
//
LOCUS       DATLBZ010000370        20211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2670213, whole genome shotgun sequence.
ACCESSION   DATLBZ010000370 DATLBZ010000000
VERSION     DATLBZ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20211
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670213"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VMZ53_18890"
     CDS             complement(<1..770)
                     /locus_tag="VMZ53_18890"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HUS30587.1"
                     /translation="MADLVVPQVGESVSEGILAKWLKQVGDAVAADEPVAELETDKVT
                     VQVLSPVAGALSEQRAKEGATVKVGESIGAVEAGKAGKAAAKPAPAAAAASAKPAPQQ
                     QAQAAAGATQTAAQPAPAKAPPPTPPAKPAQAKSGNGESLDKDKLLRLTPSQRATARE
                     TGNIPQPSSSGGSASAAPSGPSVEERLAHVDPRDEIVPMSPLRKRVAERLVQAQHESA
                     SLTTFNEVDMTAVMDLRAKYKETFEKAHGAKLGFMSFFV"
     gene            complement(821..3673)
                     /locus_tag="VMZ53_18895"
     CDS             complement(821..3673)
                     /locus_tag="VMZ53_18895"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HUS30588.1"
                     /translation="MVRDILELGGSLGFLDEMYDQFQEQPGAIDPTWHDVFNGQATDH
                     KSNGHGTNGNGASGNGHAAETNGNGKDRRTGTTRAMPMFARPGAVTMSPITAQTGSVS
                     VWPLVNAYRSRGHFAANLDPLGLLETARIVELEPATWGFTDADLNRVVEPTGVHGMPR
                     ATIGELVDHLKRVYAASAGLEFMHISSPARRSWLAERMETSLPVPPDVRTRMLALLIN
                     SEQFERFCHTKYPGTKRFSLEGSESLIPLLDLVLTHTARLGAIEAVLGMAHRGRLTTI
                     EQILKRPARDMFGHFEDIEPEKALGGGDVKYHLGFSTDRTDPNGHAMHVSLAFNPSHL
                     EAVDPVVVGRVRAKQVRHGDVEHKRVVGILVHGDAAFAGQGLVPETLQLSNLPGYRTG
                     GTIHLIVNNQVGFTASPAEQRSTPYCTDVAKMLECPIWHVNGEDLDALARVVEIACEY
                     RTQFGSDVVIDMYCYRKYGHNENDEPAFTQPIMYDRIKTKQSPIEVYSNRLISENVVT
                     PDEVTAMTQRRVAELETELEAAKAAKQRPDSPSMTAMWRGYRGGVTEAPEDVDTRVMR
                     PLLESIAQSMTEIPQGFSAHSKIVRLFEQRAAMGRGEKPLDWGMAELLAYGSLLYQHV
                     NVRLTGQDVSRGTFSHRHAVITDIVNGREHLTLGQLHPDQGQCRIYDSPLSEAGVMGF
                     EFGYSLDYPDALIMWEAQFGDFANGAQVIIDQFITSSEDKWKRLSGITLLLPHGYEGQ
                     GPEHSSARLERYLQSCAEHNIQVAQPTTPAQMFHLLRGQVLRRLRKPLIVMTPKSLLR
                     LPAATSQLEELATGTFHRVLHDDTADPAKVTRAVMCTGKIYYELADERQRLGADHVAI
                     IRIEKLYPWWPHLVAASIEKYSHLKECFWVQDEPCNMGAGPFVTPRLESVLRERGGNI
                     VYDCISRTESASPATGSHKAHVIEQAQIMKATFGPR"
     gene            complement(3706..4428)
                     /locus_tag="VMZ53_18900"
     CDS             complement(3706..4428)
                     /locus_tag="VMZ53_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30589.1"
                     /translation="MRKLALLALLPGCFLFKGKPNPSCQEGRTVELSLPEDVKKFAGC
                     EKATGIQIRTGATVDVRPLSDLEEITGDLSIGPTVGVDSVAFNGLLRVGGTIRVANNG
                     SLRGLYFPRLEHAGRIEVDNNAVLSTISMPRLAKVDGSMVITDNTGLELITTTLLVDV
                     GHELVIAGQPKLNLFEIPRIQHLQTIRLEGVPKLPPEVVEALTSKAEVNETPPAPPVI
                     APPPDAGVSVDAGTDATGAVNP"
     gene            complement(4428..5186)
                     /locus_tag="VMZ53_18905"
     CDS             complement(4428..5186)
                     /locus_tag="VMZ53_18905"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HUS30590.1"
                     /translation="MAISLSVNLNKVALLRNSRGAQNPSPKTAGIACLDNGAYGLTLH
                     WRQDERHTRKDDVRVLRHLCKERGAEFNLEGDDREELIALAEELDVTQFTLVPVKPGE
                     ITSDHGWDLPRQHDVVAPIVKRLKARGIRTAIFVDANPEMMKAAAETGVDRIEIYTEP
                     YASAWGKPELKTELARLKETAQAAVAVGMKVNCGHDLNLRNTPLLAREIPEIVEASIG
                     HALIADALYLGFPEAVRRYARVCKGEAVDAPVTQ"
     gene            5229..5846
                     /locus_tag="VMZ53_18910"
     CDS             5229..5846
                     /locus_tag="VMZ53_18910"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS30591.1"
                     /translation="MQLVACTKCNRHIRITATHCPFCSAPHTRIARSLLDAGGRLSRA
                     AVFAGAAACYTSSPQQVQGPPPPPPPDDTVQQAPPPDDSTFSQPPPGDRQPYSLGRIE
                     GTVSARGPGLNIGGIRVFVHGQNLNQDTTTDEGGRFRFEKLPPGTYTIELEGNGNPRS
                     APPRQVVTVIADGAATVTLVYSPYVPDRGPCCKPYGAPPARRRVV"
     gene            complement(5850..6893)
                     /locus_tag="VMZ53_18915"
     CDS             complement(5850..6893)
                     /locus_tag="VMZ53_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30592.1"
                     /translation="MKKALLTPGLLLLLLAAPAHADPVAHSLVVHVAPTSTTEGQPVE
                     LEAMIDAPFAETLVVHWRPIGTQAWQEGTFERSSAGGWFASIPTATSPGVEYYIQGKD
                     ASGATIDHFASEKSPHVVRVDPALFDRLEDLDRERLRGRLDEVSFDVIAHNFGNRYEL
                     KDWFIRSELTYTHKFLRQLHEAGFGFGSITGRTPVISDFPVEGGMSELTGMRYGFGQV
                     RMRLHPSVFIDGRIGLGVSHVEFQGSGAAALTFGKPWRSCVTVGGEYIGDLGGSAFVR
                     LQWDTAPPLLMGASIMRTDLPGAKIDPSGLYIAYDVSYRIEQRFTVKAQLSYGARDGA
                     AHVGGGVGTAVAF"
     gene            complement(6902..8641)
                     /locus_tag="VMZ53_18920"
     CDS             complement(6902..8641)
                     /locus_tag="VMZ53_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30593.1"
                     /translation="MKALLVIIFMALAIPAYAEPVVLAVDGKDIYVDIGAKDGVGAGS
                     ELELLHEITARDPRSGATLRDRFALGTITVIKSGAAISVARADESLAKRVLPGDRVRL
                     VSEKRAFVDPWEEQVAASKGQPAPLPGTPTVPTPTPGKAIDHAALAKDAWQDTLGKPL
                     DARITRWTELLTADPQTPYRKAIEQEINNLRYQMQLRDQALAQAKATHTDARDPRIAQ
                     LAAQLEVPVEQKQSDLVIDPINRAVPGRPIELAFLMRRPSLIRSAWLYVRADGAPGFS
                     RTELVRDGDSYLRGTIPGEQVHADSVDWYVEVFSTPDGDPMPMVGSQGAPMRIEVDTA
                     VGEAPIEQGRTHIDGHIDYVDFDGKFNDGYDQYTQAELDFTYRFLHPIYAFRVGFGTL
                     TGTGGPASVIDLGDSCLDPSGAYRCERMTFSYVYAEAEFRVRENVALMVRPTGGVLTT
                     DTMPDSASDRCKGSSNTEDCVFFTGFGARGRIRLGTELGTNLVLGASFTARVGTLLEA
                     AYHWLPNPIVPVQITVQVTDQPVVEDFGVRLIADVGVRKLSWFYPSARVSYQARNINH
                     SGFSGGLALNFDW"
     gene            complement(8642..9637)
                     /locus_tag="VMZ53_18925"
     CDS             complement(8642..9637)
                     /locus_tag="VMZ53_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30594.1"
                     /translation="MIIVLVASARIASAQVRHVPPADVEAGKDLELLAQASSSTATLV
                     LHVRARGTQPFSTIELVRKNAAAWVAVVPAISVLPPGLDYYITAGDAPVFATPEWPHT
                     IEVHATANEERRGRDVLRSANRRSRVGVMGEWVEFGNRAMTTADHYYRLDADFSYRLW
                     SYPLEEIRVGYTRLLGTTAADPTCTDANCTDAGFKVGGWFELGLAPVEGIRFDGRMAF
                     MATQDGFRVGGRGEARLGDRSASHVAVGVEYLAAVGTNGFFRLGWGTVPRVPMSATVE
                     VTNLPASHRETGVRLYYDIGTDVRPGLRLGVRVGYAARNQSVAGFTGGAGATVEF"
     gene            complement(9661..11250)
                     /locus_tag="VMZ53_18930"
     CDS             complement(9661..11250)
                     /locus_tag="VMZ53_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30595.1"
                     /translation="MRALVPIACLLIASRAYADDEIVRGNVVKIEAREIYVSIGSNRG
                     VAPGASLRIKRAVSLKHPVTRATVQDWIPIGSASVTQAGSVMSRAVVGDLTDDIKVGD
                     VAEILVDRADTQGPAPQKPVDPTPPVPQGPPVDPATAEVLGVFSAQTGQSLDARIAAW
                     ERYLSMRANSPFAADIRKDLDALHALRDELRPRNASSPDTEMIVSVDHEAPKRATSGN
                     QVPLVFVLEQPQDVASAYLHYRPRGNRTYRSMLLVREHDIYLRGTLPAEIVKSPGVDY
                     FVEVSTPSGRSGLALGTPRQPIEVAVAKPTLLDRFGSSPGLSSVKASMSYLDFATFDK
                     RAGDHTDRMIQANVDFTYKLDSAVESLGVGYGVYAGAGGEANTIWDASNPLPHAGFHY
                     GYADIEVGGRTEGVHVSAGGALIAGVGKTGFGLGAEGRLRIGEREGTNLAFIARTVEE
                     VGFLTDIRFGARPTKGMLLGISVGATNQPNRSGDVGVKLGTDIEVIAIRNVSLIVRGS
                     WQGRTTTHGGIGGGGGLGFYW"
     gene            complement(11250..11930)
                     /locus_tag="VMZ53_18935"
     CDS             complement(11250..11930)
                     /locus_tag="VMZ53_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30596.1"
                     /translation="MSRRPKVLAVVSIVALVCMTGAMGCGFGLSGPDPQRPREQMPKC
                     DTGKGAVVVDGLMATVASVVAIALVSADEPAGALIPAGIAAAYIGGAVHGSRAVDSCR
                     AAMDEWSGAIAARDQLRAGPPGDDDEPRPRRTTVAPVDPYYSPGPVAPQAPPQYPPQY
                     PPQQVPPPQVPPQQGPPQQQVPPQQVPPQQAPPPQAPQQPTRQAPPKSKQAPPSEDDW
                     SDFWREVP"
     gene            complement(12012..14945)
                     /locus_tag="VMZ53_18940"
     CDS             complement(12012..14945)
                     /locus_tag="VMZ53_18940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HUS30597.1"
                     /translation="MRHLYADFIDRVAKPMRYLGGEYQAVVKEGPDVAARVCLGFPDV
                     YDIGMSHLGTKIIYSLLNKDPRIACERAFAPWLDMEAELRQRGLPLVSLETQRALREF
                     DVLGISLQYELTFTNVLTLLDLGGIPLRSENRTSDETLVLVGGPTASHPEPIAPFIDA
                     AFVGEAEELLPSLVLAWAALRAEIRSGTRTRRDALAELASKFPLYVPSLYETVTDPET
                     GMTVVGAPLDPRVPARVNRAMVRDLEKYPFPSDAPVPYAEAVFERASVEIARGCTEGC
                     RFCQAGMIYRPVRERSPESIAKSLIDGVDKAGYDETGLTCLSTADFSSITPLVKKLAG
                     ELRKRGVAMSVASLRAYGLNEDLLDELALTRISGLTFAPEAGTQKMRDVVNKTVTEAH
                     IEESSRRVFARGWHRLKLYFMIGLPTEEDDDVRGIVETGKRMLAIGRSLVGKRAEVTV
                     SVSSHVPKPHTPFQWCAQNRLAEIQRKQQILRHSAGRDMGLRLKHHDSGVSFIEGMLA
                     RGDRRLANVIEAAWRDGARFDGWEEVFDVERWQRLLAEHDVDIAAYLGTRPVSARLPW
                     DHIDVGLEDGFLLSEYRKAMKGRASPPCGKVAGMLIHHTNLAEAQPDKRKLVCYDCGV
                     ACDLVKMKEDRLVALRVLGANERSAARDAQIDTGGVLVDVSERGGGSPGEVGEVKKSR
                     DRETERAERVRLNGHIGAGLPWTTYRIRFAKVGRAAFLGHLDLVRLLARSLRRADLSL
                     AMTRGFSPKPRISFGPALGLGVPSLGELVDVDLEHAPRGRRTWEASETDERIELSADE
                     VRDRLASVCPPGIQIQSCAIVRLNGHPLAKNPDLGLGKLIAAVDVAIKPAPDGMAHDA
                     ARLARLAEAFMAKDRVIVERGEKQIDVRALVSDVDVIAGDAATALANALDWGSAPAIF
                     RVRVSATSDGSAKPSEIARALGVWGSDDPRGEHAMVARLGVVDDLKSRPVLPVSATPL
                     HELTI"
     gene            complement(15003..15662)
                     /locus_tag="VMZ53_18945"
     CDS             complement(15003..15662)
                     /locus_tag="VMZ53_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30598.1"
                     /translation="MRLVVLLGLIAGCSFEHGRLGPTQADAEVDATGPVVIDAAIDAK
                     IDAPPTTCPDDDADSVCNDVDDWPCGAKPSTPSATVQFMNNGTATTTTISVVDLDSTG
                     RMAIVAPQTTVSLGFRLQVTDTACSGNCVDQLEAGWVDHATGLGNRFTTCLYDDPVSK
                     MNGLDTSLSFNVGAPATRKVYDLRVNLGQNFYCGANGTTGWWGGAVPPDTKTIARLCV
                     H"
     gene            15693..16370
                     /locus_tag="VMZ53_18950"
     CDS             15693..16370
                     /locus_tag="VMZ53_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HUS30599.1"
                     /translation="MRTLLLVTALAACSKTEKAPASPPPASKAQPQTMQGDTASKFDP
                     AKLPPPGTAPAPVVVTKELDSIWDIPLKTVQGKAAKLADFKGKEILVVNVASQCGNTP
                     QYATLEALQKKYEAKGFTVVGFPCNQFGGQEPGSSEEIATFCSTNYGVTFPIMEKVEV
                     NGANRSDIYKALTAIPDAGGHSGDIRWNFEKFVVSADGTKVTRFAPKTTPDDPKVIAA
                     IENGLPR"
     gene            16458..16745
                     /locus_tag="VMZ53_18955"
     CDS             16458..16745
                     /locus_tag="VMZ53_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30600.1"
                     /translation="MKKLLQITFALTLTSFAAGCHDASKDIEKYADKMCACKDAACGE
                     KVLNDFASWIKDNKNARGDQDKAAKAAERIGKCAIESGVSLDKLQSALSGL"
     gene            complement(16742..17608)
                     /locus_tag="VMZ53_18960"
     CDS             complement(16742..17608)
                     /locus_tag="VMZ53_18960"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HUS30601.1"
                     /translation="MGTNLRVVTLNFWGTEPPLDRRIALAVRQLKALDPDVVCLQEAR
                     PLDGVSGRTTADVLGEALGMHAHYSTSCAWKAGEYEGLGDGQEGLAILSKAKPLATQR
                     IELPHGRVAETRQLLSAQLPTDAAPVWVHTTHLHYRLDDGVAREAQVLAIDDAMRALG
                     RDKTSAPQILCGDFNATPDSDEMRFLRGLTTLGGRRTHFQDAWLRHHRECQPGDGHAQ
                     GITWSSENELTRPLRSLDIDRRIDYIYVTSRKKDGRATIHDCRVTLTDREDPGGICAS
                     DHYALVADVQVA"
     gene            complement(17598..17963)
                     /gene="queD"
                     /locus_tag="VMZ53_18965"
     CDS             complement(17598..17963)
                     /gene="queD"
                     /locus_tag="VMZ53_18965"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HUS30602.1"
                     /translation="MRTRLERTYRFEAAHFLPKVPPGHKCARMHGHSYQVDVAIEGEI
                     GGETGWLMDFSDIDEHVMPLVRKLDHHVLNEIEDLANPTSELLAVWLWKRLSATLSAR
                     LVEVIVSETPTSRCVYRGD"
     gene            18062..18388
                     /locus_tag="VMZ53_18970"
     CDS             18062..18388
                     /locus_tag="VMZ53_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30603.1"
                     /translation="MLLLAPPLQSSTAMTTFQTIDPSLLVTATGGQAQGPEQGDDQQP
                     QGRSWKQIAGEYTNACIQGAGQALIFGGKPRNWRQGATTAAMGCAMGVGMKALEDGGS
                     YITGSR"
     gene            18407..19765
                     /locus_tag="VMZ53_18975"
     CDS             18407..19765
                     /locus_tag="VMZ53_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30604.1"
                     /translation="MRAWIVCALCAGCGSNPTKLPTWEKSLPKADVMGTWRGLVPARG
                     IVHLHSPYSHDACDGMPRDAAGTPNEPCLDHLRKALCTTHIDYANLTDHDDTMADEEF
                     ATLFNMRGTDTPVMSNGSQIASRITCDDGHQVTVSVGGENELMPIMLERHVAGTVAER
                     HDIYNGSNATAITAMRDAGALIWVAHSEQHPHDQLMTVQPDGIEVYNLHANIDPKIRG
                     PYLGLDEYGAITNAVEFADTNEGHPEPDLALISFLEPNTPAITRWNEILADGRRIPAT
                     AGSDAHENALPVILADGERGDAYRRVLRWFGNIALVSDPSDPLQIKEALRAGRMFAVF
                     EVMGTPIGFDVHAGSAELGGEATVGTTLTVSVPSVRNLDPSLPAPEIKATVWRITATS
                     AEVVAMGPGPTVTAPMDQAGAYRVEISIVPHHLGPYLGDLGTAFADRELPWIYASPIY
                     AR"
     gene            19782..>20211
                     /locus_tag="VMZ53_18980"
     CDS             19782..>20211
                     /locus_tag="VMZ53_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30605.1"
                     /translation="MRHALLALPLLAGCLIESPSGDDTYYPPPGGGSGSGWGSGYGGS
                     DDYTDDYGCHLDTDCGTGYVCARDRECLAPSSVRIIHVNWTVKGQVPSDASCSHSPSL
                     SITFGDPLGDLFGFAPVPCKAGRYTIDKFPTRFDRVELTRQ"
BASE COUNT         3345 a         6707 c         6768 g         3391 t
ORIGIN      
        1 acgaagaacg acatgaagcc gagcttcgcg ccatgcgcct tctcgaacgt ctctttgtac
       61 tttgcgcgaa ggtccatgac cgcggtcatg tcgacttcgt tgaacgtcgt gagcgacgca
      121 gactcgtgct gcgcttggac gaggcgctcg gcgacgcgct tgcgcagcgg cgacatcggc
      181 acgatctcgt cgcgcggatc gacatgcgcg aggcgctcct cgacgctcgg ccccgacggc
      241 gcggccgatg cgctcccgcc ggacgacgat ggctgcggga tgttgccggt ctcgcgcgcg
      301 gtcgcgcgtt gcgatggtgt caggcgaagc agcttgtcct tgtcgagcga ctcgccgttt
      361 ccggacttcg cttgcgctgg tttcgccggt ggcgtcggcg gtggtgcctt cgcaggtgcg
      421 ggttgcgcgg cggtctgcgt cgcgccggca gcggcttgcg cttgctgttg cggcgcaggc
      481 ttcgcggagg cggcggcagc ggcaggtgcg ggtttcgcgg cggccttgcc tgccttaccg
      541 gcttcgacgg cgccgatcga ttcgccgacc ttcacggtcg cgccttcctt cgcgcgttgc
      601 tcgctgagcg ctccggcgac cggcgacaga acctggaccg tgaccttgtc cgtctcgagc
      661 tcggcgaccg gttcatcggc cgcgacggcg tcgcccactt gcttgagcca cttcgcgagg
      721 atgccctccg acaccgattc gccgacttgc gggacgacaa gatccgccat ggccggaatc
      781 tactccaatc cgtcggagac ggcaggtatc ggacggctat tcagcgcggc ccgaacgtag
      841 ctttcatgat ctgcgcttgc tcgatcacgt gcgccttgtg cgagccggtc gccgggctgg
      901 cgctctcggt gcgcgagatg cagtcgtaca cgatattgcc gccgcgctcg cggagcacgc
      961 tctcgaggcg aggcgtcacg aacggacctg cgcccatgtt gcagggttcg tcctgcaccc
     1021 agaagcactc cttgaggtgg ctgtacttct cgatcgacgc ggcgacgagg tgcggccacc
     1081 acgggtagag cttctcgatg cggatgatcg cgacgtggtc ggcgccgagg cgctgccgct
     1141 cgtcggcgag ctcgtaatag atcttgccgg tgcacatgac ggcgcgcgtg accttcgccg
     1201 gatcggcggt gtcgtcgtga aggacgcgat ggaacgtgcc ggtcgcgagc tcctcgagtt
     1261 gcgacgtcgc cgccgggaga cgcagcaggc ttttcggcgt catcacgatg agcggcttgc
     1321 gcaggcggcg cagcacctga ccgcggagca ggtggaacat ctgcgccggt gtcgtcggct
     1381 gagcgacctg gatgttgtgc tcggcgcacg actgcagata gcgctcgaga cgcgcgctcg
     1441 agtgctcggg cccctgccct tcatagccgt gcggcaagag cagcgtgatg cccgacaggc
     1501 gcttccactt gtcttccgac gacgtgatga actgatcgat gatcacctgc gcgccgttcg
     1561 cgaagtcgcc gaactgcgct tcccacatga tcagcgcgtc cgggtagtcg agcgaatagc
     1621 cgaactcgaa gcccatgacg cctgcttcgg agagcggcga gtcgtagatg cggcactgcc
     1681 cctgatcggg atgcagctgg ccgagcgtga ggtgctcgcg accgttcacg atgtcggtga
     1741 tgacggcgtg gcgatggctg aacgtgccgc gcgagacgtc ttggccggtg agccgaacgt
     1801 tgacgtgctg atacagcagc gagccgtacg cgaggagctc tgccattccc cagtcgagcg
     1861 gcttctcacc gcgacccatc gcagcgcgct gttcgaatag acggacgatc ttcgagtgcg
     1921 ccgagaagcc ctgcgggatc tcggtcatgc tctgcgcgat cgactcgagc agcggtcgca
     1981 tgacgcgcgt gtcgacatct tccggtgcct cggtgacacc gccgcgatag ccgcgccaca
     2041 tcgccgtcat cgacgggctg tccggacgct gcttcgccgc cttcgccgct tcgagctcgg
     2101 tctcgagctc tgcgacgcgg cgctgcgtca tcgcggtgac ttcgtcgggc gtgacgacgt
     2161 tctccgagat caggcggttc gagtagacct cgatcggaga ctgcttcgtc ttgatccggt
     2221 cgtacatgat cggctgcgtg aacgcgggct cgtcgttctc gttgtggccg tacttgcgat
     2281 agcagtacat gtcgatcacg acgtcggagc cgaactgcgt gcggtactcg caggcaatct
     2341 cgacgacgcg cgcgagcgca tcgagatcct cgccgttgac gtgccagatc gggcactcga
     2401 gcatcttcgc gacgtcggtg cagtacggcg tcgagcgctg ctcagcaggc gatgcggtga
     2461 agccgacctg gttgttgacg atcaggtgaa tcgttccgcc ggtgcggtag cccggcaggt
     2521 tcgagagctg cagcgtctcg ggcacgaggc cctggcctgc gaacgcagca tcgccgtgaa
     2581 cgaggatgcc gacgacgcgc ttgtgctcga catcgccgtg gcgcacttgc ttggcgcgga
     2641 cgcggccgac gacgaccggg tcgacggctt cgaggtggct cgggttgaac gcgagcgaga
     2701 cgtgcatcgc gtgaccattc ggatccgtgc gatccgtcga gaatccgagg tggtacttca
     2761 cgtcgccacc accgagcgcc ttctcgggct cgatgtcctc gaagtgtccg aacatgtcgc
     2821 gcgcgggccg cttcaggatt tgctcgatcg tcgtgagacg accgcggtgc gccatgccga
     2881 gcacggcctc gatcgcgccg agtcgcgcgg tgtgcgtgag cacgaggtcg aggagcggga
     2941 tgaggctctc gctgccctcg agcgagaacc gcttcgtgcc gggatacttc gtgtggcaga
     3001 agcgttcgaa ctgctcggag ttgatcagca gcgcgagcat gcgcgtgcga acgtccggtg
     3061 gcaccggcag cgaggtctcc atgcgctcgg caagccagct gcgacgagca ggcgacgaga
     3121 tgtgcatgaa ctcgagcccc gccgaggctg cgtagacgcg cttcagatga tcgacaagct
     3181 cgccgatcgt cgcgcgtggc atgccatgca cgccggtcgg ctcgacaacg cggttcagat
     3241 cggcgtcggt gaagccccac gttgcaggct cgagctcgac gatgcgtgcc gtctcgagca
     3301 gaccaagcgg atcgaggttc gctgcgaagt ggccgcggct gcgatacgcg ttgacgagcg
     3361 gccacaccga tacgctgccg gtctgcgccg tgatcggcga catcgtgacc gcgccgggtc
     3421 gcgcgaacat cggcatcgct cgcgtggtgc ccgtgcggcg atctttccca ttgccgttcg
     3481 tctcggcagc gtggccgttg ccactggcgc cgttgccatt cgtgccgtga ccgttcgact
     3541 tgtggtcggt tgcctgaccg ttgaagacgt cgtgccacgt cggatcgatc gcgccaggct
     3601 gctcctggaa ttgatcgtac atctcgtcga gaaaaccgag cgaaccgccg agctcgagaa
     3661 tgtcgcgaac catggcgggc atgataccgc caatgcgctc gatgcctacg ggttgacggc
     3721 gcctgttgcg tcggtgccag cgtcgacgga gacgcccgca tccggcggcg gcgcaatcac
     3781 gggcggcgct ggcggcgtct cgttcacctc ggccttgctc gtaagcgctt cgaccacctc
     3841 gggcggcagc ttcggcacgc cctcgaggcg aatagtctgc aagtgctgga tccgcggaat
     3901 ctcgaacagg ttgagcttcg gctggccggc gatcacgagc tcgtgaccga catcgacgag
     3961 cagcgtcgtc gtgatcagct cgaggcccgt gttgtcggtg atcaccatcg agccgtcgac
     4021 cttcgcgagg cgcggcatcg agatcgtcga gagcaccgcg ttgttgtcga cctcgatgcg
     4081 cccggcgtgc tcgagacgag ggaagtagag cccgcgcaac gagccgttgt tcgcgacgcg
     4141 gatcgtaccg ccgacgcgca ggagcccgtt gaacgcgacc gagtcgacgc cgaccgtcgg
     4201 cccgatcgac aggtcgccgg taatctcctc gagatccgaa agcggcctga catcgacggt
     4261 ggcgcccgtg cggatctgga tccccgtcgc cttctcgcaa ccggcgaact tcttcacgtc
     4321 ctcgggcaag ctgagctcga ccgtgcggcc ttcttggcac gacgggttcg gcttgccctt
     4381 gaacaggaag cagcccggca aaagcgcgag aagcgcgagc ttgcgcatca ctgcgtaaca
     4441 ggcgcgtcga ccgcctcgcc tttgcacacg cgcgcgtacc gccgcaccgc ctcgggaaag
     4501 ccgagataga gggcatccgc gatcagcgcg tggccgatcg atgcctcgac gatctccgga
     4561 atctcgcgcg cgaggagcgg cgtgttgcgc agattcagat cgtgcccgca gttgaccttc
     4621 atccctacag caacggcggc ctgcgccgtt tctttcagtc tcgcgagctc cgtcttcagc
     4681 tccggcttgc cccacgccga cgcgtacggc tccgtataaa tctcgatgcg atcgacgccg
     4741 gtctccgcgg ccgccttcat catctcggga ttcgcgtcga cgaagatcgc agtgcgaatg
     4801 ccgcgcgctt tgagccgctt cacgattggc gcaacgacat catgctggcg cggcaaatcc
     4861 caaccgtggt cgctcgtgat ctcgccgggc ttcaccggca cgagcgtgaa ctgcgtgacg
     4921 tcgagctcct cggcgagcgc gatcagctct tcgcgatcat cgccctcgag gttgaactct
     4981 gcgccgcgct ccttgcacag gtgacgcagc acgcggacat catccttgcg cgtgtgccgc
     5041 tcgtcctgtc gccagtgcaa tgtcagcccg tacgcgccgt tgtcgaggca cgcgatgccc
     5101 gctgtcttcg gcgacggatt ctgcgcaccg cgcgagttcc gaagcagtgc gaccttgttc
     5161 aggttgaccg acagagagat ggccacggcc ggttgatacc accgccgccg cccctgtagc
     5221 gtggacgcat gcagctcgtt gcgtgcacga agtgcaaccg tcacatccgg atcactgcca
     5281 cgcactgccc gttctgctcg gcgccgcaca ctcgcatcgc gcggtcgctg ctcgacgcgg
     5341 gtggccgact gtcgcgcgcc gccgtgttcg ccggtgcagc cgcttgctac acgagctcgc
     5401 cgcaacaggt gcaaggacca ccgcccccgc cgccgccgga cgacacggtg caacaagcgc
     5461 cgccgcccga cgactcgacg ttctcgcaac caccgccggg cgaccggcag ccgtattcgc
     5521 tcggccgcat cgaaggcacg gtgagcgcgc gcggccccgg cttgaacatc ggcggcatcc
     5581 gcgtctttgt gcacggccag aacctcaatc aagacacaac gaccgatgaa ggtggacgct
     5641 tccgcttcga gaagctcccg cctgggactt acaccatcga gctcgaagga aacgggaatc
     5701 cacgcagtgc accaccgcgc caagtcgtca ccgtgatcgc ggatggggcc gcgacggtca
     5761 cgctcgtcta ctctccgtac gtccccgatc gcgggccgtg ctgcaaaccg tacggcgccc
     5821 cgcccgcgcg ccggcgcgtc gtgtgagcgc tagaacgcga cggccgtacc gacgccgccg
     5881 ccgacgtgcg ctgcaccatc acgcgcgccg tacgagagct gggctttgac cgtgaaccgc
     5941 tgctcgatgc ggtagctcac gtcgtatgcg atgtagaggc ccgatggatc gatcttcgcg
     6001 cctggcagat ccgtgcgcat gatcgacgcg cccatgagga gtggcggtgc ggtgtcccac
     6061 tgcaagcgca cgaacgcaga gccgccgagg tcgccgatgt attcgccacc gacggtgacg
     6121 cacgagcgcc acggcttacc gaacgtgagc gcggccgcgc cgctgccttg gaactcgacg
     6181 tgcgagacgc cgaggccgat ccgaccgtcg atgaacaccg acgggtggag gcgcatgcgg
     6241 acttggccga agccgtagcg catcccggtg agctccgaca tgccgccctc gacggggaag
     6301 tcggagatga ccggggtgcg gcctgtgatc gagccgaagc cgaagcccgc ttcgtggagt
     6361 tggcgcagga atttgtgcgt gtacgtcagc tcgctgcgga tgaaccagtc tttgagctcg
     6421 tagcggttgc cgaagttgtg ggcgatgacg tcgaacgaga cttcgtcgag gcggccgcgc
     6481 aggcgctcgc ggtcgaggtc ttcgaggcgg tcgaacagcg cggggtcgac gcggacgacg
     6541 tggggcgact tctcggatgc gaagtgatcg atcgtcgcgc ccgaggcgtc tttgccttgg
     6601 atgtagtact cgacgcccgg gctcgtcgcc gtcgggatgc tcgcgaacca gccgcccgcg
     6661 gacgagcgct cgaacgtgcc ctcttgccag gcttgcgtgc cgatcgggcg ccagtggacg
     6721 acgagcgttt cggcgaatgg ggcgtcgatc atggcttcga gctcgaccgg ctggccttcg
     6781 gtcgtcgatg tcggagcgac gtggacgacg agcgagtgcg cgactggatc cgcgtgagcg
     6841 ggcgccgcga gcaggagcag cagcaggccg ggggtgagaa gggctttctt catggtcgtc
     6901 ctcaccagtc gaagttgagg gcaaggccac ccgagaagcc ggagtggttg atgttgcgcg
     6961 cctgatacga gacgcgagcg gatggataga accacgacag cttgcgcacg ccgacgtcgg
     7021 cgatcagacg cacgccgaag tcttcgacga ccggttggtc agtgacctgc accgtgatct
     7081 ggacggggac gatcgggttg ggcagccagt ggtacgcggc ttcgagcaac gtaccgacgc
     7141 gcgcggtgaa cgaggcgccg agcacgagat tcgtaccgag ctccgtcccg agccgaatac
     7201 gaccgcgggc gccaaagccg gtgaagaaca cgcagtcctc ggtgttcgac gagcctttgc
     7261 agcgatcgct cgcgctatcg ggcatcgtgt cggtcgtgag cacgccgccg gttgggcgaa
     7321 ccatcagcgc gacgttctcg cgcacgcgga actcggcctc tgcgtagacg taggagaacg
     7381 tcatccgctc gcagcggtag gcgcccgatg gatcgaggca cgagtcgccg aggtcgatga
     7441 cgctcgcggg gccgccggtg ccggtcaacg tgccgaagcc gacgcggaat gcgtagatcg
     7501 gatgcaggaa gcgataggtg aagtcgagct cggcctgcgt gtactggtcg tagccgtcgt
     7561 tgaacttgcc gtcgaagtcg acgtagtcga tgtggccatc gatgtgcgta cgcccctgct
     7621 cgatgggcgc ctcgcccacg gcggtgtcga cctcgatgcg catcggtgca ccttgcgagc
     7681 ccaccatcgg catcggatcg ccgtcgggcg tcgagaagac ctcgacgtac cagtcgacgc
     7741 tgtctgcatg cacttgctcg cctgggatcg tgccgcgcag gtacgagtct ccatcgcgga
     7801 cgagctcggt acgcgagaag ccgggcgcgc cgtcggcgcg aacgtagagc cacgccgagc
     7861 ggatcagcga cgggcggcgc atgaggaagg cgagctcgat cgggcggccc ggcacggcgc
     7921 gattgatcgg gtcgatgacg aggtcggact gcttctgctc gacggggacc tcgagctgtg
     7981 ccgcgagctg agcgatgcga gggtcgcgcg cgtccgtgtg cgtagccttg gcttgcgcga
     8041 gcgcctggtc gcgcagctgc atctggtagc gcaggttgtt gatctcttgc tcgatcgctt
     8101 tgcgatacgg cgtctgcgga tcggcggtga gcagctcggt ccaccgcgtg attcgcgcgt
     8161 cgagcggctt gccgagcgtg tcttgccacg cgtccttcgc gagcgccgcg tgatcgatcg
     8221 ctttcccagg cgttggcgtc ggaaccgtcg gcgttccggg cagcggtgca ggctggcctt
     8281 tgctcgccgc gacctgctcc tcccacggat cgacgaacgc tcgcttctcg gagacgaggc
     8341 gcacgcgatc gccggggagc acgcgcttgg cgagcgattc gtcggcacgc gcgacgctga
     8401 tcgccgcacc gctcttgatg accgtgatcg tgccgagcgc gaatcgatct ctcagcgttg
     8461 cgccgctgcg cggatcgcgc gccgtgattt cgtgcagcag ctcgagctca ctgcccgcgc
     8521 cgacgccgtc tttcgcgccg atgtcgacgt agatatcttt accgtcgacg gcgaggacca
     8581 ccggttctgc atacgcaggg atggccaacg ccatgaagat gatgacgagt agagctttca
     8641 tctagaactc caccgttgcg ccggcaccac cggtgaatcc ggcgaccgac tggttgcgcg
     8701 ccgcgtagcc gacgcgaacg ccgagccgca agccgggcct gacgtcggtg ccgatgtcgt
     8761 aatagaggcg caccccggtc tcgcgatgac tcgccggcag gttcgtgacc tcgacggtcg
     8821 cgctcatcgg aacgcgcggc acggtgcccc agccaaggcg gaagaagcca ttcgtgccga
     8881 cggcggccag atactcgacg ccgaccgcga cgtgactcgc gctgcggtca ccgaggcgcg
     8941 cttcgccacg accaccgacg cggaagccat cttgcgtcgc catgaacgcc atgcgcccgt
     9001 cgaaccggat gccctctacc ggtgcaaggc caagctcgaa ccagccgccg actttaaaac
     9061 ctgcgtcggt gcagttggcg tcggtgcacg tggggtcggc tgcggtcgtg ccgagcaggc
     9121 gcgtgtagcc gacgcgaatc tcttcgagcg ggtacgacca gaggcggtac gagaagtcgg
     9181 cgtcgaggcg atagtaatga tcggcggtcg tcatcgcgcg attgccgaac tcgacccact
     9241 cgcccatcac gccgacgcgc gagcgtctgt tagcgctgcg cagcacgtca cgtccgcgtc
     9301 gttcttcgtt cgcggtcgcg tgcacctcga tcgtgtgagg ccattcgggc gtcgcgaaga
     9361 ccggcgcatc acccgcggtg atgtagtagt cgaggcccgg cggcagcacg ctgatcgcag
     9421 gaaccaccgc gacccacgca gcggcgttct tgcgcacgag ctcgatcgtc gagaacggct
     9481 gggtgccgcg cgcgcgaacg tgcagcacga gcgtcgccgt cgacgacgac gcctgcgcta
     9541 gcagctccag atccttgccg gcttcgacgt cggccggtgg cacgtgccgc acttgcgccg
     9601 acgcgatgcg cgcgctcgcg acaaggacga tgatcatgat ggctgttctc atgggcgccc
     9661 tcaccagtag aaccccaggc cgccgccacc accgatgccg ccgtgggtgg tcgtgcggcc
     9721 ttgccacgag ccacgcacga tgagcgacac gtttctgatc gcaatgactt cgatatcggt
     9781 gccgagcttc acgccgacgt cgccgctgcg gttcggctga ttcgtcgcgc ccaccgagat
     9841 gcccaggagc atccccttcg tcgggcgagc gccgaatcga atatcggtga gaaagccgac
     9901 ctcctcgacg gtgcgagcga tgaacgccag gttggtgcct tcccgctcgc cgatccgcaa
     9961 cctgccctcc gcgccgagac caaacccagt cttaccaacg ccggcgatga gtgcgccgcc
    10021 cgcagaaaca tgcacgccct ccgtgcggcc gccgacctcg atgtctgcgt agccgtagtg
    10081 gaagcccgca tgcggcagtg ggttgctcgc atcccagatc gtgttcgctt cgccgccggc
    10141 acccgcgtac acgccgtagc caacgccgag gctctcgacg gcggagtcga gcttgtacgt
    10201 gaagtcgacg ttcgcctgga tcatgcgatc ggtgtgatcg ccggcgcgct tgtcgaacgt
    10261 cgcaaagtcg agatagctca tcgacgcctt caccgacgag aggcccggcg aggagccaaa
    10321 gcgatcgagg agcgtcggct tcgcgaccgc gacttcgatc ggctggcgtg gcgtgccgag
    10381 cgcgaggccc gagcggccac tgggcgtcga gacctcgacg aagtagtcga cgcctggaga
    10441 cttcacgatc tcggcgggca gcgtgccgcg cagatagatg tcgtgctcgc ggacgagcag
    10501 catgctgcga tacgtgcggt tgccgcgcgg ccggtaatgc aggtacgcgc tcgcaacgtc
    10561 ctgcggttgc tcgagcacga acacgagcgg cacttgattg ccgctggtcg cgcgcttggg
    10621 cgcctcgtga tcgacgctga cgatcatctc ggtgtcgggc gacgacgcat tgcgcggccg
    10681 aagctcgtcg cgaagcgcgt gcagtgcgtc gaggtccttg cgaatgtccg ccgcgaacgg
    10741 cgaattcgcg cgcatcgaga ggtagcgctc ccaggccgcg atccgcgcat cgagcgattg
    10801 ccccgtctgc gccgagaaca cgccgaggac ctccgccgtc gcgggatcga cgggcggtcc
    10861 ctgcggcacc ggcggcgttg ggtccacggg tttctgcggc gctgggccct gcgtgtccgc
    10921 acgatcgacg aggatctcgg cgacgtcgcc gaccttgatg tcatccgtca gatcgccgac
    10981 gaccgcgcgc gacatcaccg agcccgcctg cgtgacgctc gccgagccga tgggaatcca
    11041 gtcctgcacg gtcgcgcgcg tcaccggatg cttcagcgac accgcgcgct tgatgcgaag
    11101 cgacgccccc ggagcgacgc cgcgattcga gccaatgctg acgtagatct cgcgcgcttc
    11161 gatcttcacg acattgccac gcacgatttc gtcgtcggcg taggcgcgtg atgcgatcag
    11221 caagcacgcg atgggaacga gtgctctcat cacggcacct ctcgccagaa gtcgctccag
    11281 tcgtcctcac tgggaggcgc ctgcttcgat ttcggcggcg cttgtcgcgt cggttgctgc
    11341 ggggcttgcg gcggcggggc ctgttgcggc ggcacttgct gcggaggcac ctgctgctgc
    11401 ggcggccctt gctgcggcgg gacttgcggc ggcggcacct gctgcggtgg gtactgcggc
    11461 gggtactgcg gcggcgcctg cggggcgacg ggccccggcg agtaatatgg atcgacaggc
    11521 gcgaccgttg tgcggcgcgg gcgcggctcg tcgtcgtcgc ccggcggtcc tgcgcgaagc
    11581 tgatcgcgtg ctgcgatcgc gcccgaccat tcgtccatcg cggcgcgaca cgagtcgacc
    11641 gcgcgactgc catgcacagc gccgccgatg tacgccgctg cgatgcctgc gggaatgagc
    11701 gcaccggcgg gctcgtcggc ggagaccagc gcgatcgcga cgacgcttgc gaccgtcgcc
    11761 atcaggccat cgacgacgac ggcgcctttg cccgtatcgc atttcggcat ctgctcgcgc
    11821 ggtcgctgcg gatccgggcc ggagaggcca aaaccgcagc ccatcgcgcc ggtcatgcag
    11881 accagcgcca cgatgctcac gaccgctagg accttcggac ggcgggacat ggtcccattt
    11941 ggtagcaata gcctctgacg tcgcgaggcc ttcacgcgtt cttcacgcgg tactgccgaa
    12001 cgttgccgtt gctagatcgt caactcgtgt aagggcgtgg cactgaccgg caggaccgga
    12061 cggctcttca gatcgtcgac gacgccgaga cgcgccacca tggcgtgctc accgcgtggg
    12121 tcgtccgatc cccagacacc gagcgcacgc gcaatctccg acggcttcgc ggatccgtcc
    12181 gacgtcgcgc tgacacggac gcggaaaatc gccggcgccg agccccaatc gagcgcgttc
    12241 gcgagcgcgg ttgcggcatc gcccgcgatc acgtcgacgt cgctgacgag cgcgcgcacg
    12301 tcgatctgct tctcgccgcg ctcgacgatg acgcgatcct tcgccatgaa tgcctcggcg
    12361 aggcgcgcga gccgcgcggc gtcgtgcgcc atgccgtccg gcgcgggctt gatcgcgaca
    12421 tcgacggcag cgatgagctt gccgagcccg agatctggat tcttcgcgag cgggtgaccg
    12481 ttgagccgca cgatggcgca gctctggatc tggatgcctg gcgggcacac gctcgctagg
    12541 cggtcgcgaa cctcgtcggc cgacagctcg atgcgctcgt ccgtttccga cgcttcccac
    12601 gtgcgacgac cgcgcggtgc gtgctcgaga tcgacgtcga ccaattcgcc gagcgacggc
    12661 acgccgaggc cgagcgccgg accaaacgag atgcgcggct tcggcgagaa gccacgggtc
    12721 atcgcgagcg agaggtccgc acggcggagc gaccgcgcga gcaggcgcac gaggtcgaga
    12781 tggccgagga acgccgcgcg gccgaccttc gcaaagcgaa tgcgataggt cgtccacggc
    12841 aggcccgcgc cgatatggcc gttgaggcgc acgcgttcgg cgcgctcggt ctcgcggtcg
    12901 cgcgacttct tgacctcgcc gacttcgccg gggcttccgc cgccgcgctc cgacacatcg
    12961 actagcacgc cgcccgtatc gatctgcgcg tcgcgcgcgg cggaccgctc gttcgcaccg
    13021 agcacgcgca atgccacgag ccggtcttcc ttcatcttga cgaggtcaca cgcgacgccg
    13081 cagtcgtagc agacgagctt gcgcttatcg ggctgcgcct ctgcgaggtt cgtatgatgg
    13141 atgagcatgc cggcgacctt gccgcacggc gggctcgccc tgcccttcat cgccttgcgg
    13201 tactcgctga ggaggaatcc atcctcgaga ccgacgtcga tgtggtccca cggcaggcgc
    13261 gcactcaccg ggcgcgtgcc gagatacgcc gcgatgtcga cgtcgtgctc ggcgagcaag
    13321 cgctgccagc gctcgacgtc gaacacttct tcccagccgt cgaaccgcgc gccatcgcgc
    13381 cacgcggcct cgatgacatt ggcgagccgg cgatcaccac gcgcgagcat gccttcgatg
    13441 aaggagacgc cgctgtcgtg atgcttgagg cgcaggccca tgtcgcgccc cgcgctgtgg
    13501 cggaggatct gctgcttgcg ctggatctcg gcgagccggt tctgcgcgca ccactgaaac
    13561 ggcgtgtgcg gcttcggcac gtgcgagctg acgctgaccg tgacctccgc ccgcttgcct
    13621 acgagcgaac gcccgatcgc gagcatgcgc ttgccggtct cgacgatccc gcgcacgtcg
    13681 tcgtcttctt cggtcggcag gccgatcatg aagtacagct tcaggcgatg ccatccgcgc
    13741 gcgaacacgc ggcgcgagct ctcctcgata tgcgcctcgg tgacggtctt gttgaccacg
    13801 tcgcgcatct tctgcgtgcc ggcttcgggc gcgaacgtca ggccgctgat gcgcgtgagc
    13861 gcgagctcgt cgagcagatc ctcgttgagg ccatacgcgc gcagcgacgc gacgctcatc
    13921 gcgacaccgc gcttgcgcag ctcaccggcg agcttcttca cgagcggcgt gatgctcgag
    13981 aagtccgcgg tcgagaggca cgtcaggccg gtctcgtcgt agcccgcctt gtcgacgcca
    14041 tcgatcagcg acttcgcgat cgactccggc gagcgctcgc ggacggggcg atagatcatg
    14101 ccggcctggc agaaccggca gccttcggtg cagcctcgtg cgatctcgac cgacgcgcgc
    14161 tcgaacacgg cttcggcgta cggcaccggc gcgtccgacg ggaacggata cttctcgagg
    14221 tcgcgaacca tcgcgcggtt cacgcgtgcg ggcactcgcg gatcgagcgg cgcaccgacg
    14281 accgtcatgc ccgtctccgg atccgtcacc gtctcgtaca gcgacggcac atagagcgga
    14341 aacttcgacg cgagctcggc gagcgcatcg cgacgtgtcc gggttccgga ccgaatctct
    14401 gcacgcagcg cggcccacgc gagcacgagc gacggcagca gctcttcagc ctcaccgaca
    14461 aacgccgcgt cgatgaacgg cgcgatcggc tccgggtgcg aggcagtcgg cccaccgacg
    14521 agcacgagcg tctcgtcact cgtccgattc tcggaccgca gcgggatgcc accgaggtcg
    14581 agcagcgtga gcacgttcgt gaacgtcagc tcgtactgca gcgagatgcc gagcacgtcg
    14641 aactcgcgca gtgcgcgctg cgtctcgagc gacacgagcg gcaggccacg ctggcgcagc
    14701 tccgcttcca tgtcgagcca cggcgcgaat gcgcgctcgc acgcgatgcg cggatctttg
    14761 ttgagtagcg agtagatgat cttcgtcccg agatgggaca tgccgatgtc gtagacgtcg
    14821 gggaacccga ggcacacgcg cgccgccacg tcgggacctt ctttgacgac cgcctggtac
    14881 tcgccgccga ggtaccgcat cggcttggcg acgcgatcga tgaaatcggc atagaggtgg
    14941 cgcatggcga ccgccgtaac taccgcaaaa tgcccgcgca ggcacgcaaa ggttgataac
    15001 ccctagtgga cgcagagccg ggcgatcgtc ttggtatcgg gcgggacggc gccgccccac
    15061 cagccggtcg taccgttcgc accgcaatag aagttctggc cgaggtttac gcgcagatcg
    15121 tagaccttgc gcgttgccgg cgcgcccacg ttgaacgaca gcgacgtgtc gagcccgttc
    15181 atcttcgaga ctggatcgtc gtacaggcat gtcgtgaagc gattgccgag gcctgtcgcg
    15241 tgatcgaccc atcccgcctc gagctgatcg acacagttgc cgctgcacgc agtgtccgtg
    15301 acctgcagac ggaacccgag cgagacggtc gtctggggag ccacgatcgc catgcgtcca
    15361 gtcgagtcga ggtcgacgac ggaaatcgtc gtcgtggtcg ccgtgccgtt gttcatgaac
    15421 tgaacggtcg ccgagggtgt cgaaggcttc gcgccgcagg gccagtcgtc gacgtcgttg
    15481 cagaccgagt ccgcgtcgtc gtcggggcac gtcgttggcg gtgcatcgat cttcgcgtcg
    15541 atcgccgcat cgatcacgac gggccccgtc gcatcgacct ccgcgtcggc ctgcgttggt
    15601 ccgagccggc cgtgctcgaa gctgcaacca gcgatgagcc cgaggaggac gacgagacgc
    15661 atgacctgcc cagaatacgc taggctcgcg cgatgcggac cctcctcctg gtgacagctc
    15721 tggcagcgtg ctcgaagacc gagaaagcgc ctgctagccc accccccgca agcaaggcgc
    15781 aaccacagac aatgcaaggc gacaccgctt ccaagttcga ccccgcgaaa ctgccaccgc
    15841 caggcacggc ccctgcccct gtcgtcgtca cgaaggagct ggactcgatc tgggacatcc
    15901 cgctcaagac cgtgcaaggc aaggcggcca agctcgctga cttcaagggc aaggagatcc
    15961 tcgtcgtcaa cgtcgcgtcg cagtgcggga atacgccgca gtacgcgacg ctcgaggcgc
    16021 tccagaagaa gtacgaggcc aagggcttca cggtcgtcgg cttcccgtgc aaccagttcg
    16081 gcgggcagga gccgggctcg tccgaggaaa tcgcgacgtt ctgctccacg aactacggcg
    16141 tcaccttccc gatcatggag aaggtcgagg tcaacggcgc aaaccgcagt gacatctaca
    16201 aggcgctgac cgccatcccc gacgcaggtg ggcattcagg cgacatccgc tggaacttcg
    16261 agaaatttgt ggtgtctgcg gacggcacaa aagtgacccg ctttgcacca aagacgacgc
    16321 cggacgatcc aaaagttatc gccgcgatcg agaatggtct gcctcgctag cgctttttgc
    16381 tgcgacaagg tcgagcctcg gggctctctc agaagcaagc aaccaccaga cgcagtcacc
    16441 tgcagaacgg acgacccatg aagaagctgc tccagatcac gttcgcactc accctcacgt
    16501 ccttcgcggc tggctgccat gacgcgtcca aggacatcga gaagtacgct gacaagatgt
    16561 gcgcctgcaa agacgcggca tgcggcgaaa aggtcctcaa cgacttcgcg tcgtggatca
    16621 aggacaacaa gaacgcgcgc ggcgatcagg acaaggcggc gaaagcagcc gagcgcattg
    16681 gcaagtgcgc gatcgagtcg ggcgtctcgc tcgacaagct ccagagcgcg ctctccgggc
    16741 tctaagcgac ctgtacgtcc gcgacgagcg cgtaatgatc gctcgcgcag atgccgcctg
    16801 gatcctcgcg atcggtgagc gtcacccggc aatcgtggat tgtcgcgcgg ccgtcctttt
    16861 ttcggctcgt cacgtagatg tagtcgatgc gccgatcgat atcgagtgag cgcagcggac
    16921 gcgtcagctc gttctcgctc gaccacgtga tgccctgcgc gtgtccatcg ccgggctggc
    16981 actcgcgatg gtgacgcagc cacgcatctt ggaagtgcgt gcgtcgaccg ccgagcgtcg
    17041 tgagcccgcg caaaaagcgc atctcgtcgg aatcgggcgt cgcgttgaaa tcgccgcaca
    17101 agatctgggg agcgctcgtc ttgtcgcgac caagcgcgcg catcgcgtcg tcgatcgcga
    17161 gcacctgggc ttcgcgcgcg acaccgtcgt cgaggcggta gtgcagatgc gtcgtgtgga
    17221 cccagacggg cgcggcgtcg gtgggcagct gcgccgagag aagttggcgg gtctcggcga
    17281 cgcgaccgtg cggcagctcg atccgctgcg tcgcgagcgg cttggccttc gagagaatcg
    17341 cgaggccttc ttggccatcg ccgagtcctt cgtactcgcc cgctttccac gcgcagctcg
    17401 tcgagtagtg cgcgtgcatg ccgagcgctt cgccgagcac atcggccgtc gtacgtccgg
    17461 acacgccgtc gagcgggcgc gcttcttgga gacagacgac gtcgggatcg agcgccttca
    17521 gctggcgaac cgcgagcgcg atgcggcgat cgagcggagg ctcggtgccc cagaagttga
    17581 gggtgacgac gcggaggtta gtccccacgg tagacgcacc gcgacgtcgg cgtttccgac
    17641 acgatgactt cgacgagacg agccgacaac gtggccgaaa gccgcttcca caaccagact
    17701 gcgaggagct cgctcgttgg attggcgagg tcttcgatct cgttcagtac gtgatgatcg
    17761 agctttcgca cgagaggcat gacgtgctcg tcgatgtcgg agaaatccat cagccacccg
    17821 gtctcgccgc cgatctcgcc ttcgatcgcg acatcgacct ggtagctgtg gccgtgcatc
    17881 cgagcgcact tgtggccggg cggaaccttc ggcagaaagt gcgccgcttc gaatcgatac
    17941 gtccgttcaa ggcgcgttcg catgctccgc atgtgtaaca cgctgtcttc cgcgcgcgac
    18001 ggacggagcc ccagtcaggc ggcaacccga cgagccgctg tcacccatga ttggtttcgc
    18061 gatgttgctg ctggcaccgc cgctgcaatc gagcacggcc atgacgacct tccagaccat
    18121 cgatccttct ctcctcgtga ccgcgacggg tggccaggcg cagggcccgg aacagggcga
    18181 cgaccagcag ccgcagggcc ggtcgtggaa gcagatcgcc ggcgagtaca cgaacgcctg
    18241 catccagggc gcaggccagg cgctgatctt cggcggtaag ccgcgtaact ggcgccaggg
    18301 cgcgaccacc gcggcgatgg gctgcgcgat gggcgtcggc atgaaggcgc tcgaggacgg
    18361 cggaagctac atcaccggca gtcgctgagg tgtagattcg ggcccgatga gggcctggat
    18421 tgtgtgcgcg ctctgcgccg gctgcggcag caacccgaca aagctcccta cgtgggagaa
    18481 gtcgctgccg aaggcggacg tgatgggcac gtggcgcggc ctcgtcccgg cgcgcggcat
    18541 cgttcatctg cactcgccgt actcgcatga cgcatgcgac ggcatgccac gcgacgcagc
    18601 aggaacgccg aacgagccgt gtctcgatca tctccgcaag gcgctgtgca cgacgcacat
    18661 cgactacgcg aacctgaccg accacgacga cacgatggcg gacgaagagt tcgcgacgct
    18721 gttcaacatg cgcgggaccg atacgcccgt catgagcaac ggatcgcaga tcgcatcgcg
    18781 catcacgtgt gacgacggcc atcaagtcac cgtctccgtc ggcggcgaaa acgagctgat
    18841 gccgatcatg ctcgagcggc acgtggccgg caccgtggcc gagcgtcacg acatctacaa
    18901 cggctcgaac gcaaccgcga tcaccgcgat gcgcgacgcc ggtgcgctga tctgggtcgc
    18961 gcatagcgag cagcacccgc acgaccagct gatgaccgtg caacccgacg gcatcgaggt
    19021 ctacaacctc cacgcgaaca tcgatccgaa gatccgcggc ccgtacctcg ggctcgacga
    19081 gtacggcgcg atcacgaacg cggtcgagtt cgccgatacg aacgaaggcc atcccgagcc
    19141 cgacctcgcg ctgatctcgt tcctcgagcc gaacacgccg gcgatcacgc gctggaacga
    19201 gatcctcgcg gacggccggc gaatccccgc aaccgccggc agcgacgcgc acgagaatgc
    19261 actccccgtg atcctcgccg atggcgagcg aggcgacgcg taccgccgcg tgcttcgctg
    19321 gtttggcaac atcgcgctcg tctccgatcc gagcgacccg ctgcagatca aagaggccct
    19381 gcgcgccggc cgcatgtttg ccgtgttcga agtgatgggc acgccgatcg gcttcgatgt
    19441 ccatgccggc agcgcggagc tcggcggcga ggcaaccgtc gggaccacgc tgacggtcag
    19501 tgttccgagc gtgcgaaacc tcgatccgag tttgccagct ccggagatca aggccacggt
    19561 ctggcggatc acggcgacga gcgccgaagt cgtcgcgatg ggccccggcc cgacggtgac
    19621 cgcgccaatg gaccaagccg gtgcatatcg cgtcgagatc tcgatcgtgc cgcaccacct
    19681 cggcccgtac ctgggcgacc tcgggactgc gtttgccgac cgcgagctgc cctggattta
    19741 tgccagtccc atctatgcgc ggtgatgcta cgatttcggc catgcgtcac gcgctgctcg
    19801 cgctaccgct gttggccggg tgcctgatcg aatcgccgtc gggcgacgat acgtattacc
    19861 cgccgccggg tggtggctcg ggcagcggct ggggcagcgg ctacggcggc agcgacgact
    19921 acaccgacga ctacggctgc catctcgata ccgactgcgg caccggctat gtctgcgcgc
    19981 gcgaccgcga gtgtctggcg ccgagctcgg tccggatcat tcacgtcaac tggacggtga
    20041 agggccaggt gccgagcgac gcatcgtgct cgcactcgcc cagtctctcg atcacgttcg
    20101 gcgatcctct cggtgatctc tttggcttcg cgccggtgcc ctgcaaagcg ggccgctaca
    20161 ccatcgacaa gttcccgacg cgcttcgacc gcgtcgagct cacgcgccag g
//
LOCUS       DATLBZ010000371         5301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4905853, whole genome shotgun sequence.
ACCESSION   DATLBZ010000371 DATLBZ010000000
VERSION     DATLBZ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4905853"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2950)
                     /locus_tag="VMZ53_18985"
     CDS             complement(<1..2950)
                     /locus_tag="VMZ53_18985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HUS30606.1"
                     /translation="MRSRLNLALVVAAGSLALVLTVIRQSPEGVVSVGFDTQEVRAAP
                     GEARNQRHDLSALKIFNLVLVRIKERYVDPSRIDPRKMLYSALDSVQFNIPEVLIEPD
                     YAHNKVSVAVNDKREIFDTEDVDSPWRLSAKLKRIFRFIETNMNAGADLAKVEYSAVN
                     GMLSTLDPHSILMDPEAARDMDVSTSGKFGGLGIVIRMIDRKLMVVKPMKDTPASEKG
                     VKAGDHIVKINNELTENLTSQEAVDRMRGKPNTGVTLWVERKGVPNLLRFDLVRDVIK
                     VSQVEYKLLDKGVGYIKVKQFSKGISADVADALKEMSGKGATSWILDLRYNPGGLLEE
                     AVQLSDLFVDSGTVVTTVSGRDREARRAERGFGDTTSSLAVLVNGGSASASEIVAGAL
                     KNLDRAVIIGTRTFGKGSVQELYDNEDRSKLKLTIAQYLTPGDRSIQNLGIVPDIQLQ
                     RMYIPDKNDAPTDFVRMLAPTHTYGEKDLDAHLISTYAKETDKPAYELSFLVERKKTP
                     AVGEEPATAPPDDEDGPDEDEIVEDFEMRFAKDVVSGLAASTRPKLLSGAKTVITKYR
                     ADEEKKLVAALQTINVDWSAPATLPTANANLEVSIATTPTGTIAAGQDIAVTATVKNT
                     GLGDAYRVLSRIQADDSVFQDVELPIGHVAPGETKTFTAKVKVPADAVDRIDRLGMEV
                     REMRNAAAHVAAAELKVESLPKPVFAYSWQLIDDGNGDGLVQRGEKYKLQVSVKNTGV
                     GPTSEATVLLRNATGDGVVLEKSRVEFKDPIAPGQTKDIDFPLATDATLKADEMVLEL
                     MAYDNNLDVQASDKLRFKVSAGIKVEAKPGEVTTKQAIAIRSGASDDSPLVGGAAKGA
                     SYGSLGLYGGFTKVKLNQGGSKVGFIPTSSLSAGGSGTSTFTPYWNSTPPTIALTTKT
                     LETTGDTFKLQGTVSDEAHVEDVYIFVANSGAKIDNRKVFYRSNRSGKDAKLLDFAAD
                     LPLWPGSNMIT"
     gene            3041..4171
                     /locus_tag="VMZ53_18990"
     CDS             3041..4171
                     /locus_tag="VMZ53_18990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HUS30607.1"
                     /translation="MAERKKPGGRKKKADVIDVGDAPAKDSSEENDGDSDEEEKEEGG
                     DDDGLDADLVQSAIAVGDDDVEVLDADAEVPEPKVSTRGGSLARRDPMAAYMSETRRY
                     PLLTPEEEHALAVKLVEHGDTSAARKLIEANLRLVVKIAYEYRRAHRNLLDLVQEGNI
                     GLIQAVGKFDPYRGVKLSSYAAFWIRAYILKFILNNWRLVKIGTTQAQRKLFFNLRKE
                     REKLEQLGFAPTAALLAEKLDVPEKEVIEMERRLAAPEASLDAPMGSGGDDEGTRTRL
                     DYLPGDAEDRPDRAVAQSEFSQLLKGKLEAFAQTLQGREQTIFRERWLTEEPLTLQEI
                     GDRYNVSRERARQLEKRMLDRLKKYLEAEMGTAVDIEALTRE"
     gene            4194..5045
                     /gene="rsmI"
                     /locus_tag="VMZ53_18995"
     CDS             4194..5045
                     /gene="rsmI"
                     /locus_tag="VMZ53_18995"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HUS30608.1"
                     /translation="MPGTLFIVGTPIGNLEDLTLRAARVLREADLIAAEDTRSAKTLL
                     HHVDALGPVNGGRRLISYFEGNEAGRDTEIVDSLRAGNRVAIISEAGMPGISDPGSRA
                     VAAAIEAGARVEVIPGPNAALAALVASGLPTDRFTFFGFPPRETGARQELYGTLRNET
                     ATLIFYEAPDRVGASLADLAAALGADRRVSLGRELTKLHEEHVRGTLGELAAKYATEA
                     PRGECTLVVAGGAAAQADIDIEAELRKLLAQGLGPKDAAARLVVVTGKPRRQLYQLAL
                     SLQREKP"
     gene            5042..>5301
                     /locus_tag="VMZ53_19000"
     CDS             5042..>5301
                     /locus_tag="VMZ53_19000"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HUS30609.1"
                     /translation="MTLDVASSAEPIVFTDASSPHLTIARRTLPCGLDVIIHRDTSAP
                     QVCASVWYRVGSSDETPERTGFAHLFEHMFKNSQHMPAHHYEV"
BASE COUNT          882 a         1681 c         1784 g          954 t
ORIGIN      
        1 tggtgatcat gttcgagccc ggccagagcg gcaggtcggc ggcgaagtcg agcagcttgg
       61 cgtctttgcc gctgcggttc gagcggtaga agaccttgcg gttgtcgatc ttcgcgccgg
      121 agttcgccac gaagatgtag acgtcctcga cgtgggcttc gtccgagacg gtgccttgca
      181 gcttgaaggt gtcgcccgtg gtctcaagcg tcttcgtcgt cagcgcgatc gtcggcggcg
      241 tcgagttcca gtacggcgtg aacgtcgacg tgcccgagcc gccggcggac agcgagctcg
      301 tcgggatgaa gccgaccttg ctgccgcctt ggttgagctt gaccttcgtg aagccgccgt
      361 agaggccgag cgatccgtag ctcgcgccct tggccgcacc accgacgagc ggtgagtcgt
      421 ccgatgcgcc cgagcgaatc gcgatcgcct gcttcgtagt gacctcgccg ggtttcgcct
      481 cgaccttgat gccggccgac accttgaagc ggagcttgtc gctcgcttgg acgtcgaggt
      541 tgttgtcgta cgccatcagc tcgaggacca tctcgtcggc cttgagcgtc gcgtcggtcg
      601 cgagcgggaa gtcgatgtct ttggtctggc ccggcgcgat cggatctttg aactcgacgc
      661 ggctcttctc gaggacgacg ccgtcgccgg ttgcattgcg caggaggacg gtggcttccg
      721 acgtcgggcc gacgcccgta ttcttcaccg agacttgcag cttgtacttc tcgccgcgct
      781 ggacgaggcc atcgccgttg ccgtcatcga tgagctgcca gctgtacgcg aagacgggtt
      841 tgggcagcga ctcgaccttg agctcggcgg cggcgacgtg ggcagccgcg ttgcgcatct
      901 cgcgaacttc catgccgagg cgatcgatgc ggtcgacggc gtcggccggg actttgacct
      961 tcgcggtgaa cgtcttggtc tcgcccggtg cgacgtgacc gatcggcagc tcgacgtctt
     1021 ggaagaccga gtcgtcggct tggatgcgcg acagcacgcg gtacgcgtcg ccgaggcccg
     1081 tgttcttcac cgtcgcggtg accgcgatgt cttggcctgc ggcgatcgtg cccgtcggcg
     1141 tcgtcgcgat cgaaacttcg aggttcgcgt ttgcggtggg gagcgtcgca ggagctgacc
     1201 agtcgacgtt gatcgtctgg agcgcagcga cgagcttctt ctcttcgtcg gcgcggtact
     1261 tcgtgatgac cgtcttggcg ccggagagca gcttcgggcg cgtcgaggcc gcgaggccgg
     1321 agacgacgtc cttggcgaag cgcatctcaa agtcttcgac gatctcgtcc tcgtcgggac
     1381 cgtcttcgtc atcgggcggc gcggtggcgg gctcttcgcc cacggccggc gtcttcttgc
     1441 gctcgacgag gaacgacagc tcgtaggcag gcttgtccgt ctccttcgcg tacgtcgaga
     1501 tgaggtgcgc gtcgagatcc ttctcgccgt aggtgtgcgt cggcgcgagc atgcggacga
     1561 agtccgtcgg cgcgtcgttc ttgtcgggga tgtacatcct ctgcagctgg atgtcgggga
     1621 cgatgccgag gttctggatc gagcgatcgc caggcgtgag gtactgcgcg atcgtcagct
     1681 tgagcttgct gcgatcttcg ttgtcgtaga gctcctgcac ggagcctttg ccgaacgtgc
     1741 gcgtgccgat gatgacggcg cggtcgaggt tcttcagcgc accggcgacg atctccgacg
     1801 ccgaggccga gccgccgttg acgagcaccg cgagcgacga cgtcgtgtcg ccaaagccgc
     1861 gctctgcacg gcgagcttcg cggtcgcggc cgctgacggt cgtgacgacg gtgccgctgt
     1921 cgacgaagag atcggagagc tgcacggctt cctcgaggag gccgcccggg ttgtagcgaa
     1981 ggtcgaggat ccagctcgtc gcgcccttgc cgctcatctc tttgagcgcg tcggcgacgt
     2041 ccgccgagat gcccttggag aattgcttca ccttgatgta gccgacgccc ttatcgagca
     2101 gcttgtactc gacctgcgag accttgatga cgtcgcgcac gagatcgaag cggaggaggt
     2161 tcggcacgcc tttgcgctcg acccacagcg tgacgccggt gttcggcttg ccgcgcatgc
     2221 gatcgacggc ttcttgcgac gtgaggttct ccgtcagctc gttgttgatc ttgacgatgt
     2281 ggtcgcccgc tttgacgccc ttctccgacg ccggcgtgtc cttcatcggc ttcacgacca
     2341 tcagcttgcg gtcgatcatg cggatgacga tgccgaggcc gccgaacttg ccgctcgtcg
     2401 acacgtccat gtcgcgcgcc gcttcgggat ccatgaggat cgagtgcggg tcgagcgtcg
     2461 agagcatgcc gttgacggcc gagtactcga ccttcgcgag gtcggcgccg gcattcatgt
     2521 ttgtctcgat gaagcggaag atgcgcttga gcttcgcgga cagacgccac ggcgagtcga
     2581 catcttcggt gtcgaagatc tcgcgcttgt cattcaccgc aacggacacc ttgttgtgcg
     2641 cgtagtccgg ctcgatgagc acttcgggaa tgttgaactg cacgctgtcg agcgcgctgt
     2701 agagcatctt gcgtgggtcg atgcgcgacg ggtcgacgta gcgttccttg atgcgcacga
     2761 gcaccaagtt gaagatcttg agtgccgaca gatcgtggcg ctgattgcgc gcttcgcctg
     2821 gggcagcccg tacctcttgt gtgtcgaatc caacggagac gacaccttcg ggcgactggc
     2881 ggatcacggt cagtaccagt gccaagctcc cggccgccac cacgagggcc aggttcaacc
     2941 tgctgcgcat cagccgatta taggtgcagt cgccctgcac gccagggacg ccgaacaact
     3001 gccgacggta tgcgcatgta gtatggtcct cgggaagtcg atggccgagc gaaagaaacc
     3061 aggcggccgc aagaagaagg cggacgttat cgatgtcggt gacgcgccag ccaaggattc
     3121 ctccgaggaa aacgacggag attccgacga ggaagaaaag gaagaaggcg gcgacgacga
     3181 cggcctcgat gccgatctcg tccagtctgc gatcgcggta ggcgacgacg atgtggaagt
     3241 cctcgacgca gacgccgagg tcccggagcc aaaagtgtcg acccgcggcg gctcgctcgc
     3301 gcggcgcgac ccgatggccg cctatatgtc cgagacgcgg cgctacccgc tgctcactcc
     3361 cgaagaggag cacgcgctcg cggtcaagct cgtcgagcat ggcgacacct cggcggctcg
     3421 caagctcatc gaggcgaacc tccgcctcgt cgtgaagatc gcctacgagt accgccgagc
     3481 gcacagaaac ctgctcgacc tcgtgcagga aggcaacatc ggcctcatcc aggcggtcgg
     3541 caaattcgat ccgtatcgag gcgtgaagct gtcgagctac gcggcgttct ggattcgcgc
     3601 gtacatcctc aagttcatcc tgaacaactg gcggctcgtc aaaatcggca ccacgcaggc
     3661 gcagcgcaag ctgttcttca acctgcgcaa ggagcgcgag aagctcgagc agctgggctt
     3721 cgcgccgacg gccgcgctgc tcgccgagaa gctcgacgtc cccgaaaaag aagtcatcga
     3781 gatggagcgc cggctcgccg cgcccgaggc atcgctcgat gcaccgatgg gcagtggcgg
     3841 cgacgacgaa ggcacgcgca cgcgcctcga ctacctcccc ggcgacgccg aagatcgccc
     3901 cgatcgcgcg gtggcacaga gcgagttctc gcagctgctc aaaggcaagc tcgaggcgtt
     3961 cgcgcagacg ctgcaaggtc gcgagcagac gatcttccgc gagcgctggc tgaccgagga
     4021 gccgctcacg ctgcaagaga tcggcgatcg ctacaacgtg tcgcgtgaac gcgcgcgcca
     4081 gctcgagaag cgcatgctcg atcggctgaa gaagtatctc gaggccgaga tgggcacggc
     4141 ggtcgacatc gaggcgctca cgcgcgaatg acgggcaccg cgaaatcttt tccatgcccg
     4201 gcacgctctt catcgtcggt acgccgatcg gcaatctcga agacctgacg ctgcgtgcgg
     4261 cgcgtgttct gcgcgaagcc gatctgatcg ctgcggagga cacgcgttcc gcgaagacgc
     4321 tcctccatca cgtcgacgcg ctggggcccg tgaacggcgg gcgccgcctc atctcgtact
     4381 tcgagggcaa cgaggccggc cgcgataccg agattgtcga ctcgctgcgt gcaggcaatc
     4441 gcgtcgcgat catcagtgaa gcggggatgc ccggcatcag cgatcccggc tcgcgtgcgg
     4501 tggccgcggc gatcgaagcg ggcgcgcgcg tcgaagtgat tccaggcccc aatgcggcgc
     4561 tcgcggcgct cgtcgcgtcg gggctgccga ccgatcgctt cacgttcttc ggatttccgc
     4621 cgagggagac cggcgcgcgg caggagctgt acggcacgct gcgcaacgag accgcgacgc
     4681 tgatcttcta cgaggcgccc gatcgcgtcg gtgcatcact cgcggatctg gcagcggcgc
     4741 tcggcgcgga tcggcgcgta tcgctcgggc gcgagcttac gaagctgcac gaagagcacg
     4801 tgcgcggcac gctcggcgag ctcgcggcga agtatgcgac cgaggcgccg cgcggcgaat
     4861 gcacgctcgt cgtcgcaggt ggcgcagccg cgcaagcgga catcgatatc gaggcggagc
     4921 tgcgcaagct gctcgcgcaa ggcctcgggc cgaaagacgc ggcagcgcgc ctcgtcgtgg
     4981 tgaccggcaa accgcgcaga cagctgtacc agctcgcgct atctcttcag cgagagaagc
     5041 catgaccctc gacgtcgcga gctcggccga accgatcgtc ttcaccgacg cgtcgtcgcc
     5101 gcatctcacg atcgcgcggc gcacgctgcc atgcggcctc gacgtgatca ttcatcgcga
     5161 cacctcggcg ccgcaggtct gcgccagcgt ctggtatcgc gtcggcagct ctgacgagac
     5221 gcccgagcgc accggcttcg cgcacctgtt cgagcacatg ttcaagaaca gccagcacat
     5281 gccggcgcac cactacgagg t
//
LOCUS       DATLBZ010000372        27738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8417247, whole genome shotgun sequence.
ACCESSION   DATLBZ010000372 DATLBZ010000000
VERSION     DATLBZ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 27738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27738
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8417247"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VMZ53_19005"
     CDS             <1..193
                     /locus_tag="VMZ53_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30610.1"
                     /translation="ARGIAFERWPIDLGGSFDDVWQFLAASYGHPDDDRAIREALRAE
                     FSGEHVPCTAVTFLATVRR"
     gene            complement(203..1297)
                     /gene="ychF"
                     /locus_tag="VMZ53_19010"
     CDS             complement(203..1297)
                     /gene="ychF"
                     /locus_tag="VMZ53_19010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HUS30611.1"
                     /translation="MALSVGIVGLPNVGKSTVFNALTAGKAEAANYPFCTIDPNVGVV
                     PVPDGRLKRIQKHIPTQKVIPAIVEIVDIAGLVKGASQGEGLGNKFLANIRETNAILM
                     MVRCFEDPNVIHVSGSVDPMRDIDIIDLELALADVDSADKRLKKAAGNAKSGGKEAKV
                     ELALVEKVYKHLSDGKAARSLALSDDERKQVSTWGLLTAKPVLYCCNVGEADLPAGNK
                     WSDAVKARAKQEAAGVVVLCGKVEAELAEVAEADRGELLSAYGLEEPALATLARECYR
                     LLGLQSYFTAGEKEIRAWTIRKGAMGPEAAGVIHTDFEKGFIRANVYSLADLEAHGSE
                     AGIKAAGKMRVEGKEYVVQDGDIMHFLFNV"
     gene            1347..2753
                     /locus_tag="VMZ53_19015"
     CDS             1347..2753
                     /locus_tag="VMZ53_19015"
                     /inference="COORDINATES: protein motif:HMM:NF017396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070008 - serine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HUS30612.1"
                     /translation="MRLFVVAAALLIACGDDSKPPGIMEPDPADFLQMLQALPGVHDA
                     TAEPTQNANFTYYVVHFEQPVDHDDPSSPTFLQEVSLLHRDINAPMVVWTSGYWDYYR
                     DYDTELTQLLVGNQISIEHRYFAESRPANPDWTKLTIAQMAADEHAIIAAFRTMYPGA
                     FLTSGGSKGGMTAVYHRRFYPDDVDGTVPYVAPISFGAPDLRYAAFLDTLGPQACRQA
                     VRDLATEMLANRRAAMLTRTSAQAQVDNTTYTRIPIGPAVESAIVSLEWSFWQYYGVN
                     YCTMVPATTASDDKLFEFLDEVSPPSDNSDERIAEFDAYYYQAYYQLGYPDGGAAYLD
                     SHLMYTDADYNGALPTDMPAYDGGAAMMDVDQFVREQGERLLFVYGEWDPWTGGEFEL
                     GNAKDSLRVVQTQGTHGASIRRLEDADRAAAFAKLEAWTGVAPVVTARVAARHVEAPR
                     VPPALRLAFRARRLSPQP"
     gene            2777..3598
                     /locus_tag="VMZ53_19020"
     CDS             2777..3598
                     /locus_tag="VMZ53_19020"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS30613.1"
                     /translation="MSERVVLVTGSTDGIGRQTARQLAANGHKVIVHGRSKTKVDAAV
                     AALREELPGAQLDGVAFDMGTFAGVRRGAGQVLELAPKLHVLVNNAGIFASERVVNSD
                     EIETTFMVNYLGPFLLTELLGPRLTESADGGPPSRVINVASVAHTRGRIHFDDLQLTN
                     SWTGYAAYAQSKLAQVMHAMTLADRHEPAKLLAYSLHPGVVGTKLLRQGFGPVQGIST
                     EQGAKTSVMLAGGLSENEPSGSYYSDGVATQPSSNARDEDARQKLWDKSVELAKP"
     gene            3638..4894
                     /locus_tag="VMZ53_19025"
     CDS             3638..4894
                     /locus_tag="VMZ53_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30614.1"
                     /translation="MLAAGDARTALVHVRAHLGWPRGKEVGELVGWMRLLGEVAGKLG
                     ATALEDIAKAAVRDPDSPDRLYDLGYALIDAGAPSIAASVLWHCLKLVGDSEEVVCEL
                     VSALESALAYNDAFAVLEQHAALRTRSFLCEYLYAFNAAMTGRLDTTRSVLSRLAPDS
                     PETEQMKTTIAGIVERADLISTTSTLDSKDLRGWQYALTGTLVTHQSPFGFDEPMHGR
                     YAWLQDSLARVVTGIERLAPLVRALNVACVYPAPGRDHEILAHAIAKRLELPIAPWPA
                     IGIPAPGLIVVYDLGTLERGELGRLQQRKDNQILFAHASPWTRDMPVAPDVTTLLYQS
                     LVPPWAESTIVDPETKQVTFAPPDNRPAEEVAADLLASPGLDADDRACDEPLKWSALV
                     ERTWPPPPGIRARWWAGGPVASNRFD"
     gene            4958..5326
                     /locus_tag="VMZ53_19030"
     CDS             4958..5326
                     /locus_tag="VMZ53_19030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS30615.1"
                     /translation="MKKILVVEDDPVNQMILMDFLAANGYQTIAASSGPEGIEQFDKT
                     TPDLCLVDVQLPRKNGFELMREIKSKASTPILLMSAVYTDQDASTRTVQLGTLADGYL
                     TKPFDLVQLLAQVRQLLGEA"
     gene            5328..6725
                     /locus_tag="VMZ53_19035"
     CDS             5328..6725
                     /locus_tag="VMZ53_19035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS30616.1"
                     /translation="MKTPAFIAEAIAPANRNKLRFGIYTGYAMAAMFAILAATSSVFE
                     LTPWRWAFIGLISTKLLTTSVAWLALVRDRFVATTQVISSTADVVLLTGAIYFTGGPY
                     SPLIATYVIVVAAVALLSNVGATLVLCGGIVLAFTSMMIGMATGILPPTAEPGAPGQI
                     PTAGYTLTAIIYCIFVVGVPAYFCAAILSTLRTKERDLEARTAQLIAANTQRSQFVAS
                     MTHELRTPIHGVQGLADVIAAGVYGPVTDKQKEAVATIKRSAQSLLVLVDDVLNLARA
                     DANRIEAHPTPIDIGALVGRVVASVTWMIGTKKINLASDVEPELPFVQSDDRWLAHVL
                     VNLVSNAVKFTPEGGTVTVRAYERQEHDAVVLEVKDTGIGISRDDRHRIFEPFRQADD
                     KADTKGFGGVGLGLALVARLTDLLGAKVELDSEVSKGSTFRVIVPLDYKGRAVSRVMR
                     AVVPPADADLSQLEK"
     gene            complement(6705..7568)
                     /locus_tag="VMZ53_19040"
     CDS             complement(6705..7568)
                     /locus_tag="VMZ53_19040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS30617.1"
                     /translation="MRSRRLALSTGLTYHVLDWDAAKDALHPDTTFVLVHGFLDLAFG
                     WREVAERIAKRAHVVAPDLRGHGDSDWIGPGGYYHYMDYCADLDALIPRVVRKRLVLV
                     GHSMGGGVASYWAGTRPDKIAALALLEGIGPPDQDGAQLPARTAQWIDAWRGARTKSV
                     KPMSSLADAAARLRKHDPLLGEALALELAKAGTRPVEDGLAWKHDPLHLTMGPYPFRR
                     EAAEQYWKRVTCPVLVVDGEKTMLNLPEAERAVRRAAFANHRHVVLPGAGHMMQRHQP
                     AALAELLLELA"
     gene            complement(7573..8952)
                     /locus_tag="VMZ53_19045"
     CDS             complement(7573..8952)
                     /locus_tag="VMZ53_19045"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUS30618.1"
                     /translation="MRQTALAVLLLAAACGDDSGAKSDGNNGPQDAPIDMPAIDAPAL
                     PACTPVSGTNVTVRKVTQITSEGGALLVTSPPNDGRLFVVMQSGKVMIVENEQVRATP
                     FLDISNSNIAAGNPPSEQGLLGLAFHPNYAGTREVYVMYTTGNADVVARFHADPADPY
                     KVESNTAEIILSIPDFAGNHNGGMIEFGKDGYLYIGTGDGGGGGDPRHNGQQLLRSNC
                     QVTGCEPLLGKLLRIDISGADGVKNYKIPADNPYADGVAGVPEIYALGLRNPWRWAFD
                     KQTGDIWIGDVGQSGTTGRNTLGFEEIDYVPFADLKGKNFGWSVWEGGVNVTNPATDV
                     HCYCSNPGTNTNCNYTCTATGMTFPQVERDHANDGWAAIIGGDVYRGTCYPDLVGTYF
                     FGDNSKRVLSKATLSGTTVTATDLPAPVGNWPSGIASIHKDARGELYLTTANAATTSG
                     SIYHIEAGP"
     gene            complement(8962..9327)
                     /locus_tag="VMZ53_19050"
     CDS             complement(8962..9327)
                     /locus_tag="VMZ53_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30619.1"
                     /translation="MSEKPVPDVEQAFDSDKHLELAKAIEKLNPEEAAYFLWKLEIAI
                     RKRKIQIMGYLVAMLVWLGGMLFALVYYGTHDGFVGWAFLLPFVLVGAILFSFGKWSD
                     RIGKSKPPENVSVPVDKKR"
     gene            9362..10207
                     /locus_tag="VMZ53_19055"
     CDS             9362..10207
                     /locus_tag="VMZ53_19055"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HUS30620.1"
                     /translation="MPPLKVTDDLGRSLLFPHPPQRIVSLVPSDTYSVVALGAGNRLV
                     GRTTYCEHGDGVATVGGTKDVDVEKVLALEPHLVIANQEENSRRPLEQLAERVPVLVS
                     LPRRVDAGIGHLARIARILEVGDDPKVKELVKRGYELPKAPEAKLRAFVPIWMDPLMT
                     LNGETFGSDVLARVGVSNAFGDRPRLYPLAADLGKAMPQDAAGRDDRYPRITLDEIKQ
                     RGADVIVLPDEPHAFSAADEALLQETLPSAKVIRVSGKDLFWYGAWTIEALARLEAQL
                     HSSAN"
     gene            complement(10204..11022)
                     /locus_tag="VMZ53_19060"
     CDS             complement(10204..11022)
                     /locus_tag="VMZ53_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30621.1"
                     /translation="MKLRVATCTTLPEVDVDEAPLMSALAAANIDAELVAWDDPAADW
                     DAPIPTIIRSTWNYALDLEAFLAWVDRAAAAAPLINPPDVVRANVRKRYLLELASRGV
                     PVIRTILLGKCDQLPAMAGRFVIKPEVGAGSLDTKLFDKLDDAARAHAGHITARGNAL
                     MQPYVRSVEEYGERSVIFIDGELTHEIRKSPRFSGDTESVTGPFPIADDERAVAEAAL
                     EPYRDRILYGRVDMARDDSGQPMVMELELVEPSLFFAKHPPALDRYIAGLKRRL"
     gene            complement(11019..12143)
                     /locus_tag="VMZ53_19065"
     CDS             complement(11019..12143)
                     /locus_tag="VMZ53_19065"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HUS30622.1"
                     /translation="MTLRPETLAAQAVHAFDEATGAIVPPIHLATTYARDADYQLIAD
                     RDYARDKNPTFLAAEATLAALEHGAEARVFASGMAAATVAIRSIVKPGDHIVAPRVGY
                     FALRNWLARFAAKWQVGLDLVDMTDIAAVRAVIKKGSTRLVWVETPANPTWDVTDIAE
                     VAKLAHEAGAVVVVDSTCATPVHSQPLSLGADLVMHSATKYLGGHSDVLAGALITKVT
                     DITWTEITQTRHDEGAVLGPVEAWLLLRGMRTLYARVERQSRTAQMLAERLSQLPDVA
                     VHYPGLHGHPQHDVAQTQMKGGFGGMLSIRVGARDPLDVVKKLRVWLPATSLGGVESL
                     VEHRATVEGPASPTPKDLLRLSVGLEHEEDLFEDLRQALA"
     gene            complement(12140..12910)
                     /gene="modA"
                     /locus_tag="VMZ53_19070"
     CDS             complement(12140..12910)
                     /gene="modA"
                     /locus_tag="VMZ53_19070"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HUS30623.1"
                     /translation="MRRLLLPLLILIGAACSKGQPSNAPVKEFRVAAASDLAKAFDEV
                     GKAYTKQSGVTPQFDFGSSGLFAKQIEQGAPFSLYAAANKNFAEQVVKAGKCDAASLQ
                     LYSRGRIVLWGKNGSPARLEELADPKYKRIGIANPDHAPYGKAAIEALQKAGVYDQVK
                     DRLVMAENVLAAHTYAKEGQVDVSFSALSLAIAQDSQAYLAIPPDLHTPLEQSMVVCG
                     KGPAADAARDFAKFIVSDAGRAIMEKYGFSTTGVLPAK"
     gene            12965..13669
                     /locus_tag="VMZ53_19075"
     CDS             12965..13669
                     /locus_tag="VMZ53_19075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS30624.1"
                     /translation="MERRVVIIEDERDVSRLLEFNLQQAGYTVHVAGTGGEGLALVRA
                     HLPDVVVLDLMLPDTSGYEVCKEIRGDMKIADTCVLMLTAKGEAEDRILGLEVGADDY
                     VVKPFVVREVVLRVTALANRLAERRARPAQSSGGTAIKVGPIELDPVTHDVRINNVPT
                     QLRPLEYKLLQLLVSDPGRVFTREELLEQVWEMRGDINTRTVDVHVRRLRVSLGPAAD
                     VIETVHGFGYRARRDA"
     gene            13685..15355
                     /locus_tag="VMZ53_19080"
     CDS             13685..15355
                     /locus_tag="VMZ53_19080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS30625.1"
                     /translation="MLRSLRNRLVALFVVLVVVVGGLTLWRIESTLADDLLSALDARL
                     QRQGQAVADWLKIAGHVDRLTPRLADVTGTRLTIIGADGTVQGDSQDPTLVGKKLDPA
                     WELVQAQKGKVARVVRELAPNEPKQYVVAVPAEMGRVIRLAVPLGDVIETRARMRNRL
                     LVGFFFGFLGALALSWIFVRAVTKPIQSMTRTAEALAAGKYDVATPTAAVDAGGELEV
                     LARAMIHMAGEVKARVGELTEQRDLLSVIFGGLVEGVVVVAPDGSILVSNAAAAPLIG
                     EALPESLKSLVERARKGEQPEQELELVGRTVRASAKPLHTVNPSIIVVLYDVTRMRAL
                     ENVRREFLSNAAHELRTPVTSIAGYAETLIGGGVDAELQKEFITTIHRNARRIADMVT
                     DLLLLDQLGGRAAVVSERGSIKLSEVVDDAVRTAKGVKPTAQIDVTVEAELGVLATRE
                     GLDHVVQNLIDNAVKYGNGTPVKVTAKREGKRVRLAIEDAGPGIPKGHDDRIFERFYR
                     VDAGRSRDQGGSGLGLAIVKSHVEAMGGRVWFEHADPGARFVIELDAA"
     gene            15413..18580
                     /locus_tag="VMZ53_19085"
     CDS             15413..18580
                     /locus_tag="VMZ53_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30626.1"
                     /translation="MAEQPPKTPGAQSNKTEKLGETGISRLGPAPRVGDKPLGPSPRP
                     IATPPPGVTPRSSADVLTPSPAEAVKPSRTAHDRGLEVRMAIGHAETAPHADGENLEI
                     DPGMRLRQYELIRELGRGGMGQVFLARDVKLARRVAIKFLSTASRELTERFLREARTT
                     AAVNHENIVVIHEVDEHAGMPHMVLEYLEGTSLRDLMRGRAYSAGRVVELIVPVVRAV
                     ARAHAAGIVHRDLKPENIFVTIDGAIKVLDFGIAKALVDPDAVPHKANASDLEHLAKD
                     ALTGKSAIIGTPQYMSPEQFGLDVVDHRSDIWALGIMMFELLTGKHPLAPFTIHSLLT
                     AAANVDERMPAIGSVVHDLPEKLERIVDRCLAKRKGERFSDAKSLLAELESLVPGKLG
                     RKLRDDESPYPGLSAFQEDDADRFFGRGREVMRLQTRLRDHGLLAIAGPSGVGKSSFV
                     RAGIVPALKRMGETWETYILRPGRQPMASLAGVLAPLISASGEGVRTTNPNALADYET
                     LVARLTAEPGYLGQLLRERAARKNAHVLLFVDQFEELYTLVPDEAQRRAFTACLAGAA
                     DDASSPIRIICSLRSDFLDRVAEDRAFLDELMRGLIFLQPLGRPELREALSRPLDAHG
                     YRYESDELLEQMLDELAAVPGALPLLQYAGARLWDARDRKNKVLTASAHAAMGGIAGA
                     LATHADQFLAGLPADSHRLVRAIFQRLVTPERTRAIAELEDLEALATDPADTRRIIDQ
                     LVAARLLVVQVHGETGGASVEIVHESLLDAWPTLRRWLDEDQEDAAFLAQLATAAKQW
                     ESKGRAQGLLWRGDAMLEAQRWAKTRARRLPDRDQAYLDAVLALARRTRRVRFAAVVA
                     AFAVMTAIAGGASFAYTRVRAANEVAEHQRQVAEKALVDKIAEETARRQAEDQRTAAL
                     ASLLTEEQLRKAAEDGLITAEQLAQIAEIQKRVAQKKLVTSEQQRNQLAEQLRKSEAE
                     KRAAEAAAQQKSAEAQMTREQLVEKNQQLEAALSSAKTAREKAEALRADSDRISAQLK
                     QALAAKQAEVERLEKERKKITTDLK"
     gene            complement(18596..19489)
                     /locus_tag="VMZ53_19090"
     CDS             complement(18596..19489)
                     /locus_tag="VMZ53_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30627.1"
                     /translation="MAQFTGGPSVRVSANNSVEFKWLTDVAWFGKVEIFTTSDGNGAP
                     VLIKNSEDAGGNPIKATQQIVTLPVGPALQQDTGYFFRITATDPTLANADLVASAPLP
                     PLFCGNQMVSTVSATPTTNSATVAWQSNVPGFGKVAYGTNGLTQSVQDGFNICAHALD
                     LPALQPGTTYKFQVSNRHAIDGDDLVTAQGQFTTAAVSQSVVFTEPHAEPRVIQEKQT
                     SAVSIRTKNQGAVVAGVQVTFAIAAGSAGAGTLSVTQATTDANGIATVQFTGTSRGLV
                     KVAVSAANASNSPHEIPVVVK"
     gene            19534..22434
                     /locus_tag="VMZ53_19095"
     CDS             19534..22434
                     /locus_tag="VMZ53_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30628.1"
                     /translation="MVGLRAIGIAMVVVIAGAGCLAFDDVQCADGHLCPPGSRCDDVH
                     GGCILPSQLQACVGIPDGGDCQIDGADGACRNGACVAFTCGDGLRDGVEPCEGDDFGG
                     MTCTDFGFTNPDGLACTDDCHINKDNCTGGCGDNIKDQTELCDGTDIPAATTCQTLGF
                     YDAAGLECSPFCTFDVAKCTGFCGDGAQNGPEQCDAFAPAGQTCLDYSFDGGRLGCTL
                     CAPGFEGCTSFGWKRIQSPATQRLRGIWGTSSSNFYIVGLAGTLLRYNGSQLSTVVPA
                     PTTQDLNAVWGSDASNIFAVGASGTILRFDGSAWTSTTSGSALLLGVWGSSANDVYAV
                     GSGGTVLHYAGSWAPVMGTGAGTNDLRAVWGIGSHVFAVGTSGTVLHYDSGTWTATAI
                     AGSPDLFGVWGTSATNVYASGTTATSNTAVYRFDGFTWTLVYQGGQGGRFNAVWAASA
                     TDAFAVGTAIGHFDGSKWSPLLLPSDVSAGTRLWGVWGSPTGDVWAIGDAGYVARWGG
                     ASWTVPQVTPATTVSFRGVSTWGSSDAFAVGLGAIARYDGASWTITNTTSATDQLYGV
                     WAASQSFAVAVGETSTSSAGIIYHFDGNSWINLPSGDSRNCGGLPSFGLRSVWGSSTS
                     NIYSVGGGTSGIFRRFATCWSAPTAQPSGAMQLFGIHGTSATNAFAASVGGLVYAFDG
                     ASWTSLPTPPTTSFLITVWTSGANDLFAVGDAILHYDGTNWARMSANGQNLGGVSGTG
                     PRNVFAVGNNSRLLHYDGISWTPMRVPTSIASTYLRSVSATQRSLLVVGDNGFMQRLA
                     FSQRSSEVNCRDAWDDDADGMPDCADTDCAADAYCKRGGACSVIRDVTCGQTITGSTL
                     GATPGRDYYTCDSNAETGPEAIYRLVPPATGTATATLSAFAPAELDLVVVGAMPSTDA
                     CDPDINCDGASSTDGTATEAVTFGTTMNRPEFIIVEGRNAAAGPFTLKVTCQ"
     gene            22431..23387
                     /locus_tag="VMZ53_19100"
     CDS             22431..23387
                     /locus_tag="VMZ53_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30629.1"
                     /translation="MMRWILLVLAFTVASAHADNEPWRVGVTPERMQAAKKLLDEGND
                     LLLARDWQAALVKYQAAIEQWDHPGIRFNIVKCLVQLDRTIEAVDNLDLALKYGAAPL
                     DETVYSEALGYQKLLANQVATIAVECKQPGVEVTLDGQPLDACPGTSKRRVLPNRHQV
                     VGKRAGFLTHTVEIFVLGGKTEAVSVALDPVGTSGTMVHRWSTWVPWAVVGAGVVAGG
                     LGGLSQARAVANRDAYEDKLVGCGAAGCPDGFQSGLRERATLENRLAIGLWVTAGVAI
                     VTGGTMLYMNRGRTVYTKQTEKIEPTAAITPGGATVGLRGSF"
     gene            complement(23379..23972)
                     /locus_tag="VMZ53_19105"
     CDS             complement(23379..23972)
                     /locus_tag="VMZ53_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30630.1"
                     /translation="MGARSLILAGALAACGPKADPMVPAPATIHQKVARDFEQAVKTS
                     KDAYVSLFDFVAVGQYEILIHRYDLNGRFPNLPDDVRARFEAEDGTPYPPERERRNVG
                     NFYTILAQRTVGTGGCTGAAPMTEYGRQLGEEFEPLPEGTPPGYETLRTAANGYLAKG
                     GVVGIRCSGGKGGLAVVYTERPNARGYELITIYDDSE"
     gene            complement(23954..24832)
                     /locus_tag="VMZ53_19110"
     CDS             complement(23954..24832)
                     /locus_tag="VMZ53_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUS30631.1"
                     /translation="MADDVLSYDDKVDERYEPTSPWWLSGTPLEAEHHRLMRRRSTPI
                     PWDASTAEKLTPAERMRLAGTWMRRSEAEYLAVSTFSVLAIDLVAAQAPADVISLCLR
                     AGIDEVRHAEMCVRMAKIYSGEENLPVPGMSALPDDPSRPKLHQALANTMLVSCVSET
                     YATTVLSATRDLTTDPTAQAVLTAIYSDEVMHARLGWSYMRHAIEIGGKGVIDAAAEM
                     LPIALRGVANVVERERPIGDVTDAVRTHGLMTPAEERVIYSTCVREVLVPGFQALGIP
                     IGSALEDYGDAWAHAA"
     gene            complement(24839..25315)
                     /locus_tag="VMZ53_19115"
     CDS             complement(24839..25315)
                     /locus_tag="VMZ53_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30632.1"
                     /translation="MGKHRELAKELYSSRIWRTVVFAGAMLGTPGSAVAEQAAAPPPP
                     QKQAPVATQPANPPAKPAPPSAGAPAQIVAAPQPAKPDPITVKAAALSSANSERNTLL
                     DKLNSVEPKEIDKLKKEIDAKDAQIKKLVTELVNLRKPRPRTPPDVRPVGRGFVLA"
     gene            complement(25406..25855)
                     /locus_tag="VMZ53_19120"
     CDS             complement(25406..25855)
                     /locus_tag="VMZ53_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30633.1"
                     /translation="MANHRDLAKELYASSLGRTSASRIWRTVVFAGAMLGAPLATTAC
                     GGGSKPATTTPAGGGDTAAQDQAAQDQAAQQQADAAKAAADKEAADKAAADAAAADQA
                     KKDEEARLAQEQADKEAAEKAAAEEAAAQKRARGTGKRPTGRGFVLA"
     gene            complement(25953..>27738)
                     /locus_tag="VMZ53_19125"
     CDS             complement(25953..>27738)
                     /locus_tag="VMZ53_19125"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUS30634.1"
                     /translation="NAFYCFAGQPTLQKLPAWVVTGWGLIVVFASTTIFMRRISRRED
                     QYVQEKFAQKILKKWEWGDAPPSDDLKDIYLLHTERTKQREARLRAYKETVREMVADG
                     LVTRGELVILDSLRAQLGVSDKDHQKILGELSAEERQLFDPAYQGSVEQRLAKQQYRK
                     DLERIVVEAARAGSAPADSTLEALRNERGVDADAEKAALDELLAPGGPIAAIYRGELA
                     EMERLVTVAEACATPADAANSDSASLALLRHLAQRRAHEHAVHALGVLGVMTKRPEVE
                     SVRQHAKARNLIDPSRLVELAPVDESLRKPLVDLLTRLVREDRGDFKAEPLLAVARDA
                     SPHLRAAVAMLLSRFEDAGARATLLEMLDDVDAIVREAAVRAMGAKSRLTRELLAKVL
                     NDPDATVRHTAVRAVSGTTSGEIPVVDITKLAQTTMGVGKAGAYATLDANAAMSSLTT
                     IEKMMLLRQVPIFTELDADDLEELAHIVEERIVMGDKDLFRQGDAGDAVYLIVKGKVR
                     VLVGGTDGRPETTINELGPGACIGEMAVLDASPRSATVRSLERTRALRVPGEGFKRLM
                     TERPEMSEAIVAELVRRLRDVTTKMAQK"
BASE COUNT         4524 a         9116 c         9280 g         4818 t
ORIGIN      
        1 cgcgcgtgga atcgcgttcg agcggtggcc catcgacctc ggcggcagct tcgacgacgt
       61 ctggcagttt ctcgcggcga gctacgggca tcccgacgac gatcgggcga tccgcgaagc
      121 gctgcgcgcc gagttttcgg gcgagcacgt gccgtgcacc gcggtgacgt ttctcgcgac
      181 ggtgcggcgg tagcagcgac ggtcagacgt tgaagaggaa gtgcatgatg tcgccgtcct
      241 gcacgacgta ctctttgcct tccacgcgca tctttccggc ggccttgatg ccggcctcgc
      301 tgccgtgcgc ttcgagatcc gcgagcgagt agacgttcgc gcggatgaag cctttttcga
      361 agtcggtgtg gatcacgccg gcggcttcgg ggcccatcgc gcctttgcgg atcgtccacg
      421 cgcggatctc tttttcgccg gccgtgaagt acgactgcag gccgaggagg cggtagcact
      481 cgcgagcgag cgttgcgagg gcgggctctt cgaggccgta ggcggagagc agctcgccac
      541 gatcggcttc ggcgacttcg gccagctcgg cttcgacctt gccgcagagg acgacgacgc
      601 cggcggcttc ctgcttcgcg cgcgctttga cggcgtcgct ccacttgttg ccggcgggga
      661 ggtcggcctc gcccacgttg cagcagtaga ggaccggctt ggccgtgagc aggccccacg
      721 tcgagacttg cttgcgttcg tcgtcggata gcgcgagcga gcgcgcggct ttgccgtcgg
      781 agaggtgctt gtagactttc tcgacgaggg cgagctcgac cttcgcttct ttgccgcctg
      841 acttggcgtt gcctgcggct tttttgaggc gcttgtcggc ggagtcgacg tcggcgagcg
      901 cgagctcgag atcgatgatg tcgatgtcgc gcatcggatc gaccgagccg ctcacgtgga
      961 tgacgttcgg gtcctcgaag cagcgcacca tcatgaggat ggcgttcgtc tcgcggatgt
     1021 ttgcgaggaa cttgttgccg aggccttcgc cttgcgacgc gcccttgacg aggcctgcga
     1081 tgtccacgat ctcgacgatg gccgggatca ccttttgcgt cgggatgtgt ttttggatcc
     1141 gcttgaggcg gccgtcgggg acggggacca cgccgacgtt tgggtcgatc gtgcagaacg
     1201 ggtagttcgc tgcctcggct ttgccagcag tgagcgcgtt gaagaccgtg ctcttgccca
     1261 cattggggag gccgacgatg ccgacggaaa gcgccatggg agcgaatgcc taccccggag
     1321 atccatggcg tggcaagctg ccgccgatgc gccttttcgt ggttgctgcc gctttgctga
     1381 ttgcctgcgg cgatgacagc aagcccccgg ggatcatgga gccggacccg gcggacttcc
     1441 tgcagatgct gcaggcgttg ccgggggtgc atgacgcgac cgcagaaccc acgcagaacg
     1501 ccaacttcac gtactacgtc gtgcacttcg agcagccggt cgaccacgac gatccgagca
     1561 gtccgacgtt tctgcaggag gtctcgctcc tgcaccgcga catcaacgcg cccatggtcg
     1621 tctggaccag cggctactgg gactactacc gcgactacga tacggagctg acccagctcc
     1681 tcgtcggcaa ccagatctcg atcgagcacc ggtacttcgc cgagtcgcgg cccgcaaacc
     1741 ccgattggac gaagctgacg atcgcgcaga tggccgccga cgagcacgcg atcatcgccg
     1801 cgtttcgcac gatgtatccc ggcgcgtttc tgacgagcgg cgggagcaaa ggcggcatga
     1861 cggccgtcta tcaccgccgg ttctatcccg atgacgtcga cggcaccgtg ccgtacgtcg
     1921 cgccgatttc gttcggagcg cccgacctgc gctacgcggc gtttctcgac acgctcggac
     1981 cgcaggcgtg ccggcaggcg gtgcgcgacc tcgcgaccga gatgctcgcg aatcgacgag
     2041 cggcgatgct gacgcgcacc tcggcgcagg ctcaggtcga taacaccacg tacacgcgca
     2101 tcccgattgg acccgccgtc gagtcggcga tcgtcagcct cgagtggtcg ttctggcagt
     2161 actacggcgt caactactgc accatggtgc cggcgaccac ggcgagcgat gacaagctgt
     2221 tcgagttcct cgacgaggtt tcgccgccgt ccgacaactc ggacgagcgc atcgccgagt
     2281 tcgacgccta ctactaccag gcgtactacc agctcggtta tcccgatggt ggcgcggcgt
     2341 atctcgactc gcatctcatg tacaccgacg ccgattacaa cggcgcattg ccgacggaca
     2401 tgccggcgta tgacggcggt gcggcaatga tggacgtcga ccagttcgtc cgcgagcagg
     2461 gcgagcggct gctgttcgtc tacggcgagt gggatccgtg gaccggcggc gaattcgagc
     2521 tcggcaatgc gaaggattcg ctgcgagtcg tgcaaacgca gggcacgcac ggtgcgagca
     2581 ttcgcaggct cgaggacgcc gaccgcgcag cggcgtttgc gaagctcgag gcctggaccg
     2641 gcgtcgcgcc cgtcgtgacc gcgcgggtcg cagctcgcca cgtcgaagcg ccgagggtgc
     2701 cgcccgcgtt gcggcttgcg ttccgcgcgc gtcgactctc gccacagccc tgagcgccta
     2761 gtgataaggt cggcgtatgt ccgagcgcgt cgtcctcgtc accgggtcca ccgacggtat
     2821 cggtcgccag actgcgcgcc agctcgccgc gaatggtcac aaggtgatcg ttcacggccg
     2881 cagcaagacg aaagtcgatg cagccgtcgc ggcgctgcgc gaggagctgc cgggcgcaca
     2941 gctcgacggc gtcgcgttcg acatggggac gttcgcaggc gtgcgtcgcg gtgccggaca
     3001 ggtgctcgag ctcgcaccga agctgcacgt gctcgtcaac aacgccggca tcttcgcgag
     3061 cgaacgcgtc gtcaactccg acgagatcga gacgacgttc atggtgaatt acctcggtcc
     3121 attcctgctg accgagctgc tcgggccgcg gctcaccgag agcgccgatg gcgggccgcc
     3181 gtcgcgcgtg atcaacgtgg cgtcggtcgc gcacacgcgc ggtcgcatcc atttcgatga
     3241 cctgcagctc acgaattcct ggaccggcta cgcggcgtac gcgcagagca agctcgcgca
     3301 ggtgatgcac gcgatgacgc tcgccgatcg ccatgaaccg gcgaagctcc tcgcatacag
     3361 cctgcatccc ggtgtcgtcg gcacgaagct cttgcgccaa ggctttggcc ccgtgcaggg
     3421 catctcgacg gagcagggtg cgaagacgag cgtcatgctc gcaggcggtc tctccgagaa
     3481 cgagccctcg ggcagctact acagcgacgg cgtggcaacg cagccctcgt cgaacgcgcg
     3541 cgatgaggac gctcggcaaa agctgtggga caaatccgtc gagctcgcga agccttgaat
     3601 gactgtggga agcgacgagc tggcgcaagg ggaggcgatg ctcgctgcgg gcgatgcgcg
     3661 cactgcgctc gtgcatgttc gcgcccacct tggttggcct cgcggtaaag aggtcggcga
     3721 gctcgtcggt tggatgcggc tgctcggcga agttgccggg aagctcggcg cgacggccct
     3781 cgaagacatc gccaaagctg cggtccggga tccggacagt ccagatcggc tgtacgacct
     3841 cggctacgca ttgatcgacg ctggtgcgcc atcgatcgcg gcgagcgtgc tgtggcactg
     3901 cctcaagctc gtcggcgaca gcgaagaggt cgtgtgcgag ctggtgtcgg cgctcgagag
     3961 tgcgctcgcg tacaacgatg cgttcgcggt cctcgagcag catgcggcac tgcgcacgcg
     4021 cagctttttg tgcgagtacc tctacgcgtt caacgcggcg atgaccggtc gcctcgacac
     4081 gacgcgcagc gtgctctcgc gccttgcccc ggactcgccg gagaccgagc agatgaagac
     4141 gacgatcgcc ggcatcgtcg agcgcgcgga tctcatcagc accacgtcga cgctcgacag
     4201 caaagatctg cgcggctggc agtacgcgct gaccggcacg ctcgtcacgc atcaatcgcc
     4261 gtttggcttc gacgagccga tgcacggtcg ctacgcgtgg ctgcaggatt cgctcgctcg
     4321 cgtcgtgaca ggcatcgaga gattggcgcc gctcgttcgc gcgctgaatg tcgcctgcgt
     4381 gtatccggcg cccggtcgcg atcacgagat cctcgcgcac gcgatcgcga agcggctgga
     4441 gctccccatc gcaccgtggc cggccatcgg catccctgcg ccgggcctca tcgtcgtcta
     4501 cgacctcggt acgctcgagc gcggcgagct cggcaggctg cagcaacgca aggacaatca
     4561 gatcctgttc gcgcacgcga gcccgtggac gcgggacatg ccggtcgccc ccgacgtgac
     4621 gacgctgctc tatcaatcgc tcgtcccgcc gtgggctgaa tcgacgatcg tcgatcccga
     4681 gacgaagcag gtcacgttcg cgcctccgga caatcgaccg gccgaggagg tcgccgccga
     4741 tctgctcgcg agcccgggtc tcgacgcgga cgatcgcgca tgcgatgagc ccctcaaatg
     4801 gtccgcactc gtcgagcgca cgtggccgcc gccgccgggc attcgagcga ggtggtgggc
     4861 aggtgggccc gtcgcctcca atcgatttga ctgaaaggcg ccgtacgcaa aggcgcgagg
     4921 ctgattaatc gcgggcgtct ggtgttacgc tcggggcgtg aagaagatcc tggtcgtcga
     4981 ggacgatccg gtaaaccaga tgatcctgat ggacttcctg gctgccaatg gctaccagac
     5041 catcgcggcg agctctggtc ccgaggggat cgagcagttc gacaagacga cgccggacct
     5101 gtgtctcgtc gacgttcagc tgccgcgtaa gaacggcttc gagctgatgc gcgagatcaa
     5161 gagcaaggcc tcgacgccga tcctgctgat gagcgcggtc tacaccgatc aagacgcgtc
     5221 gacgcgcacc gtgcagctcg gcacgctcgc ggatggatat ttgacgaagc cgttcgacct
     5281 cgtccagttg ctcgcgcaag tgcgccagtt gctcggcgag gcgtagcgtg aagacaccgg
     5341 cgttcatcgc cgaagcgatc gcaccggcga accgcaacaa gctccggttc ggcatctaca
     5401 ccggctacgc gatggccgcg atgttcgcca tactcgccgc gacgtcctct gtcttcgagc
     5461 tgacgccgtg gcggtgggca ttcatcggcc tcatctcgac gaagctgctc acgacatccg
     5521 tcgcgtggct cgcgctcgtc cgcgaccggt tcgtcgccac gacgcaggtc atcagctcga
     5581 cggccgacgt tgtcctcctc accggagcga tctatttcac cggtgggccc tacagccccc
     5641 tcattgccac ctacgtgatc gttgtcgctg ccgtcgccct gctctcgaac gtcggcgcaa
     5701 cgctcgtgct gtgcggcggc atcgtcctcg cgttcacgag catgatgatc ggtatggcga
     5761 cgggcatcct gccgccgacc gccgagccgg gcgctccagg ccagataccg acggccggct
     5821 acacgctcac cgcgatcatc tactgcatct tcgtcgtcgg cgtgcccgcg tacttctgcg
     5881 cggcgatcct gagcacgctg cgcacgaaag aacgcgatct cgaagcgcgc accgcgcagc
     5941 tcatcgcagc aaacacgcag cgcagccaat tcgtcgcctc gatgacgcac gagctgcgca
     6001 cgcccatcca cggcgtccaa ggcctggccg acgtgatcgc agccggcgtc tacggtccag
     6061 tcacggacaa gcagaaggaa gcggtcgcca cgatcaagcg cagtgcgcaa tcgctgctcg
     6121 tgctcgtcga cgacgtgctc aacctcgcgc gtgccgacgc gaatcgcatc gaagcgcatc
     6181 cgaccccgat cgatatcggc gcgctcgtcg gtcgcgtggt cgcatcggtg acgtggatga
     6241 tcggcacgaa gaagatcaac ctcgcgagtg acgtcgagcc cgagctgcca ttcgtgcagt
     6301 ccgacgatcg gtggctcgcg cacgtcctcg tcaacctcgt cagcaacgcc gtcaagttca
     6361 cgcccgaagg cggcaccgtc accgtccgcg cgtacgagcg ccaggagcac gacgccgtcg
     6421 tcctcgaagt gaaagacacc ggcatcggca tctcgcgcga cgatcgacat cgcatcttcg
     6481 agccgttccg ccaagccgac gacaaagcag acaccaaagg cttcggcggc gtcggcctcg
     6541 gcctcgcgct cgtcgcccgc ctgaccgacc tcctcggcgc caaggtcgag ctcgactccg
     6601 aagtcagcaa gggctcgacg ttccgcgtga tcgtgccgct cgattacaaa ggccgcgccg
     6661 tcagccgcgt gatgcgcgcc gtggtcccac ccgccgacgc cgacttaagc cagctcgaga
     6721 agtagctcgg cgagcgcggc cggctgatgc cgctgcatca tgtgcccggc acccggcagc
     6781 acgacgtggc gatgattcgc aaatgccgca cgtcggactg cgcgctcggc ctccggcaaa
     6841 ttcagcatcg tcttctcgcc gtcgacgacg agcaccggac acgtgacgcg cttccaatat
     6901 tgctcggcgg cttcgcgccg aaacgggtag ggccccatcg tcagatgcag cgggtcgtgc
     6961 ttccacgcga ggccatcttc gacgggccgc gtccccgcct tcgcgagctc gagcgcgagc
     7021 gcttcgccga gcaacggatc atgcttgcgc aaccgcgcgg ccgcatcggc gagtgacgac
     7081 atcggcttca cactcttcgt gcgcgcgcct cgccacgcgt cgatccactg cgcggtacgc
     7141 gccggcagct gcgcgccatc ttgatcgggc ggtccgatac cttcgagcag tgcgagcgcg
     7201 gcgatcttgt cgggacgcgt gcctgcccaa tagctcgcga cgccgccgcc catcgagtgc
     7261 ccgacgagca ccagccgttt gcgcacgacc cgagggatga gcgcgtcgag atccgcgcag
     7321 tagtccatgt agtggtagta gccgccgggc ccgatccaat cgctgtcgcc gtgaccgcgc
     7381 agatccggcg cgacgacatg cgcgcgcttc gcgatccgct cggcgacctc acgccagccg
     7441 aaagcgagat cgagaaagcc gtggacgagc acgaacgtcg tgtcggggtg gagcgcgtct
     7501 ttcgcagcgt cccagtcgag cacgtgatag gtcagcccgg tcgagagcgc gagccgacgc
     7561 gagcgcacag cgttagggtc ccgcctcgat gtgatagatg ctgccgctcg tcgtcgcggc
     7621 gttcgcggtc gtcaggtaga gctcgccgcg cgcgtccttg tggatgctcg cgatacccga
     7681 cggccagttg ccgaccggcg ccgggagatc cgttgcggtg accgtcgtgc cgctgagcgt
     7741 tgccttcgag agcactcgct tcgagttgtc gccgaagaag tacgtgccga cgagatccgg
     7801 gtagcacgtc ccacggtaga catcgccacc gatgatcgcg gcccagccat cgttcgcgtg
     7861 atcgcgctcg acctgcggga acgtcatacc ggtcgccgtg caggtgtagt tgcagttcgt
     7921 gttcgtgccc gggttgctgc agtagcagtg gacgtccgtt gctgggttcg tgacgttcac
     7981 gccgccttcc cacacgctcc agccgaagtt cttgcctttg agatccgcga acgggacgta
     8041 gtcgatctct tcgaagccga gcgtgttgcg gccggtggtt cccgactggc cgacgtcacc
     8101 gatccagatg tcaccggtct gcttgtcgaa tgcccagcgc cacgggttgc gcaaaccgag
     8161 cgcgtagatt tcggggacgc ccgcgacgcc gtcggcgtac gggttgtcgg cgggaatctt
     8221 gtagttcttc acgccgtcgg ctccgctgat gtcgatgcgg aggagcttgc cgaggagcgg
     8281 ctcgcatccc gtgacttggc agttcgagcg aaggagctgc tgcccgttgt gcctcggatc
     8341 gccgccaccg ccgccatcgc cggtgccgat gtagaggtag ccgtccttgc cgaactcgat
     8401 catgccgccg ttgtggtttc ccgcgaagtc gggaatcgac aggatgatct cggcggtgtt
     8461 cgactcgacc ttgtatggat cggcggggtc ggcgtggaag cgtgcgacca cgtccgcgtt
     8521 gccagtcgtg tacatgacgt acacctcgcg cgtgcccgcg tagttcgggt ggaacgcgag
     8581 accgaggagg ccttgctcgc tcggcgggtt gcccgcggcg atgttcgaat tcgagatatc
     8641 gaggaacggc gtcgcgcgca cttgctcgtt ctcgacgatc atcaccttgc cgctctgcat
     8701 gacgacgaag aggcggccgt cgttgggtgg cgaggtgacg agcagtgcgc cgccttccga
     8761 ggtgatctgc gtgaccttgc gaaccgtgac gttcgtgccg ctgactggcg tgcacgctgg
     8821 tagggccggc gcatcgatcg cgggcatgtc gatgggcgcg tcctgcggac cattgttccc
     8881 gtcggatttc gcgccgctat catccccgca cgctgcagcg agcaggagaa ccgcaagggc
     8941 cgtttgcctc atgcggcgat actagcgctt cttgtcgacc gggacggaaa cgttctcggg
     9001 gggcttggac ttaccgatcc tgtccgacca cttgccgaat gagaacagga tcgcgccaac
     9061 cagcacgaac ggcagcagga acgcccatcc gacgaagcca tcgtgcgtcc cgtaatagac
     9121 gagagcgaat agcattcctc cgagccagac gagcatcgcg acgagatagc ccatgatctg
     9181 gatcttgcgc ttgcggatcg cgatctcgag cttccacagg aagtacgcag cttcctcggg
     9241 attcagtttc tcgatcgctt tggcgagctc taggtgcttg tccgaatcga acgcttgttc
     9301 gacatcaggc accggcttct cgctcacggc gtgaccttag catgtgtggt aagtcccgcc
     9361 ggtgcctcca ctgaaagtca ccgacgatct ggggcggtcg ctgctctttc cgcatccgcc
     9421 gcagcgcatc gtgtctctcg tcccgagcga tacgtactcc gtcgtggcgc tcggcgcggg
     9481 caatcgcctc gtcggtcgaa cgacgtactg cgagcacggc gatggtgtcg cgacggttgg
     9541 cggcacgaag gacgtcgatg tcgagaaggt gctcgcgctc gagccgcacc tggtgatcgc
     9601 gaatcaggag gagaactcgc gcaggccgct cgagcagctc gccgaacgcg tgccggtgct
     9661 cgtgtcgctg ccgcgccgcg tggatgcggg catcggtcac ctcgcgcgga tcgcgcgcat
     9721 cctcgaggtc ggcgatgatc cgaaggtgaa ggagctcgtg aagcgcggct acgagctgcc
     9781 gaaggcacca gaggcgaagc tgcgcgcgtt cgtgccgatc tggatggatc cgctcatgac
     9841 gctcaacggc gagacgtttg gctccgatgt gctcgcgcgc gtcggcgtct ccaatgcatt
     9901 cggcgatcgc ccgcgcttgt atccactcgc cgcggatctc ggtaaggcga tgccgcagga
     9961 cgcggcaggc cgcgacgatc gctatccacg catcacgctc gacgagatca agcagcgcgg
    10021 cgccgatgtg atcgtgctgc cagacgagcc acacgcattc tcggccgccg acgaagctct
    10081 gctgcaggag acgttgccga gcgcgaaggt gatccgcgtg agcggcaagg atctgttctg
    10141 gtacggcgcg tggacgatcg aggcgctcgc ccggctcgag gcgcagctgc actcgtccgc
    10201 aaattaaagc cggcgcttca ggcccgcgat gtagcgatcg agcgcgggcg gatgcttcgc
    10261 gaagaacagc gacggctcga cgagctcgag ctccatcacc atcggttggc cgctgtcgtc
    10321 gcgcgccatg tcgacgcggc cgtagaggat gcgatcgcga tacggctcga gtgcggcttc
    10381 cgcgaccgcg cgttcgtcgt cggcgatcgg gaagggcccg gtcacgctct cggtgtcgcc
    10441 ggagaaccgc ggtgacttgc gaatctcgtg cgtgagctca ccgtcgatga agatgaccga
    10501 gcgctcgccg tattcttcga ccgagcgaac gtacggctgc atcagtgcgt tgccgcgagc
    10561 cgtgatgtga ccggcgtgag cgcgcgcagc gtcgtcgagc ttgtcgaata gcttcgtgtc
    10621 gagcgagccc gcaccgactt cgggcttgat cacgaagcgg cctgccatcg cggggagctg
    10681 gtcgcacttg ccgagcagga tcgtgcggat cacgggcacg ccgcgagatg cgagctcgag
    10741 caggtagcgc ttgcgcacgt tcgcgcgcac gacatcggga ggattgatga gcggcgctgc
    10801 tgcggctgct cggtcgaccc acgcgagaaa tgcctcgagg tcgagcgcgt agttccacgt
    10861 cgagcggatg atcgtcggaa tcggcgcgtc ccagtctgct gccgggtcat cccaggcgac
    10921 aagctcggca tcgatgttcg ccgcagcgag cgcgctcatc agcggcgctt cgtcgacgtc
    10981 gacttcgggc agcgtcgtac aggtcgcaac gcgcagcttc atgcgagcgc ctgccgcaga
    11041 tcctcgaaga gatcctcttc gtgctcgagg ccgacggaca gccgcaataa atccttcggc
    11101 gtcggcgatg ctggcccctc gacggtcgcg cgatgctcga ccagcgattc gacgccacct
    11161 agtgacgtcg cgggcagcca cacccgcagt ttcttgacga cgtcgagagg atcgcgcgcg
    11221 ccgacgcgga tcgagagcat cccgccgaag ccgcccttca tctgcgtctg cgcaacgtcg
    11281 tgctgcggat gcccgtgcag ccccggatag tggaccgcca cgtcggggag ctgcgagagc
    11341 cgctcggcga gcatctgcgc ggtccggctc tgccgctcga cgcgggcata cagcgtgcgc
    11401 atgccgcgca ggagcagcca cgcttcgacg ggcccgagca ccgcaccttc atcgtgccgc
    11461 gtctgcgtga tctccgtcca cgtgatgtcg gtgaccttcg tgatcagcgc gcccgcgagg
    11521 acgtcggagt gaccgccgag atactttgtc gccgagtgca tgacgagatc cgcgccgagc
    11581 gacagcggtt gcgagtgcac gggtgtcgcg cacgtcgaat cgacgacgac gaccgcgccg
    11641 gcctcatggg cgagcttggc aacctcggcg atatcggtga cgtcccacgt cggattcgcc
    11701 ggcgtctcga cccagaccag gcgcgtcgag cctttcttga tcacggcgcg cacggcggcg
    11761 atgtccgtca tgtcgacgag atcgagcccg acctgccact tcgcagcgaa ccgcgcgagc
    11821 cagttgcgca gcgcgaagta accgacgcgc ggggcgacga tgtgatcgcc cggcttcacg
    11881 atcgagcgaa tcgcgacggt cgcggccgcc atccccgatg caaacacgcg cgcttcggcc
    11941 ccatgctcga gcgcggcgag cgtcgcctcc gcggcgagga atgtcgggtt cttgtcgcgc
    12001 gcgtaatcgc ggtccgcgat caattgatag tcggcgtcgc gcgcgtacgt cgtcgcgaga
    12061 tggatcggcg gcacgatcgc gccggtcgct tcgtcgaacg cgtggactgc ttgcgcggcg
    12121 agcgtctcgg gtcgcagcgt catttcgcgg gcagcacgcc ggtcgtcgag aacccgtact
    12181 tctccatgat cgcgcggcct gcatcactca cgatgaactt cgcaaagtcg cgcgccgcat
    12241 cggccgccgg acccttgccg cagacgacca tcgattgctc gagcggcgtg tgcagatccg
    12301 gcgggatcgc gagatacgcc tgcgagtcct gcgcgatcgc gagcgacagc gcgctgaacg
    12361 acacgtcgac ctggccttct ttcgcgtacg tgtgcgcggc gagaacgttc tcggccatca
    12421 cgagccggtc tttcacctga tcgtagacgc cggctttctg cagcgcctcg atcgccgcct
    12481 tgccgtaggg cgcgtggtca gggttcgcga tgccgatgcg cttgtacttc ggatcggcga
    12541 gctcctcgag gcgcgccggc gagccgttct tgccccacag cacgatgcga ccgcgcgagt
    12601 agagctgcag cgaggcggcg tcgcacttgc ccgctttcac gacttgctcg gcgaagttct
    12661 tgttcgcggc tgcgtacagc gagaacggcg cgccttgttc gatctgcttc gcgaatagac
    12721 cgctcgagcc gaagtcgaac tgcggtgtga cgccgctctg tttcgtgtac gccttgccga
    12781 cctcatcgaa cgctttcgcg aggtccgatg ccgcggcgac gcggaactcc ttcaccggtg
    12841 cgttcgacgg ctgtcccttc gagcaggcag cgccgatgag gatcaaaagg ggcagtagaa
    12901 ggcgtcgcat caccggagcg tatgccgacg acgccgccca ttcaatcgat gtataacgag
    12961 catcatggaa cgtcgggtcg tgatcatcga agacgagcgc gacgtgtcgc ggttgctcga
    13021 gttcaatctg caacaggccg gttacacggt tcacgttgcg ggcaccggcg gtgaaggcct
    13081 cgcgttagtt cgcgcgcact tgcccgacgt cgtcgtgctc gacctgatgc tgcccgatac
    13141 gtcgggctac gaggtctgca aggagatccg cggcgacatg aagatcgctg acacctgcgt
    13201 gctcatgctg acagccaagg gcgaagcaga agatcggatt ttaggactcg aggttggcgc
    13261 cgacgactac gtggtgaagc cgttcgtcgt gcgcgaggtc gtgctgcgcg tgacagcgct
    13321 cgcgaatcgt ctcgccgaac gccgcgcacg tcccgcgcag agcagcggcg gcaccgcgat
    13381 caaagtcggc ccgatcgagc tcgatcccgt tacgcatgac gtccgcatca acaacgtgcc
    13441 aacgcaactg cgtccgctcg agtacaagct gctccagctg ctggtctctg accccggtcg
    13501 ggtgttcacg cgcgaagagc tcctcgagca agtgtgggaa atgcgcggcg acatcaatac
    13561 gcgcaccgtc gatgttcacg tccgccgcct gcgcgtcagc ctcggtcccg ctgccgatgt
    13621 gatcgagacg gtccacggct tcggctaccg cgcgcgccgc gacgcatagg atcgtgctca
    13681 cacagtgctt cgctctctcc gcaaccgact cgtcgcactg ttcgtagtgc tcgtcgtcgt
    13741 cgtcggtggg ctcacgctgt ggcgcatcga gagcacgctc gccgatgatc tgctgtcggc
    13801 actcgacgcg cggttgcagc gccagggcca ggcggtggca gactggctga agatcgcagg
    13861 tcacgtcgat cgcctcacgc cgcgcctcgc ggatgtcacc ggcacgcggc tcacgattat
    13921 cggcgcggat ggcacggtgc agggcgactc gcaggatccg acgctcgtcg gcaagaagct
    13981 cgatcccgcc tgggagctcg tgcaagcgca gaagggcaag gtcgcacgcg tcgttcgcga
    14041 gctcgcgccg aacgagccga agcaatacgt cgtcgcagtg ccggccgaga tgggacgcgt
    14101 gatccggctc gcggtgccgc tcggtgacgt gatcgagacg cgcgcacgca tgcgcaaccg
    14161 gctgctcgtc ggatttttct tcggcttcct cggcgcgctc gcgctgtcgt ggatcttcgt
    14221 ccgcgcggtg acgaagccga tccaatcgat gacgcggacg gccgaagcgc tcgctgctgg
    14281 taagtacgat gtcgcgacgc caaccgccgc tgtcgacgca ggtggcgagc tcgaagtgct
    14341 cgcgcgggcg atgattcaca tggcaggcga ggtgaaggcg cgggtcggcg agctgaccga
    14401 gcagcgcgat ctgctgtcgg tgatctttgg cggtctcgtc gaaggcgtcg tcgtcgtcgc
    14461 gcccgatggc tcgatcctcg tgtcgaacgc cgccgctgcg ccgctgatcg gcgaggcgtt
    14521 gcccgagtcg ctgaagtcgc tcgtcgaacg cgcgcgcaag ggcgagcagc cagagcaaga
    14581 gctcgagctc gtcggtcgga ccgtccgcgc gagcgcgaag cctctgcaca ccgtcaaccc
    14641 gtcgatcatc gtcgtgctct acgacgtgac gcgcatgcga gcgctcgaga acgttcgccg
    14701 cgagttcttg tcgaacgctg cgcacgagct gcggacgccg gtgacctcga tcgccggtta
    14761 tgccgagacg ctcatcggcg gcggcgtcga tgcggagctg caaaaagagt tcatcacgac
    14821 gatccatcgc aacgcgcgac gcatcgccga tatggtgacg gacctcctgc tgctcgatca
    14881 gctcggtggt cgcgctgccg tcgtcagcga gcgagggtcg atcaagctgt cggaggtcgt
    14941 cgacgatgcc gtgcgcacgg cgaagggcgt gaagccgacc gcgcagatcg acgtgaccgt
    15001 cgaagccgag ctcggcgtcc tggcgactcg cgaaggcctc gaccacgtcg tacaaaatct
    15061 gatcgacaac gcggtcaagt acggcaacgg aacgcccgtc aaagtcaccg cgaagcggga
    15121 gggcaagcgc gtgcggctcg cgatcgagga cgccggccca ggcattccca agggccacga
    15181 cgatcggatc ttcgagcggt tctatcgcgt cgacgcaggt cgtagccgcg accaaggcgg
    15241 gtctggcctc ggcctcgcga tcgtgaagag ccacgtcgag gcgatgggtg gccgcgtgtg
    15301 gttcgaacac gccgatccag gtgcgcggtt cgtgatcgag ctcgacgccg cgtagacctc
    15361 gcgctcgaat gcgtggtcca accacgcgct ggtgtacgat gggtattggc gaatggccga
    15421 gcaaccaccg aagacgccgg gtgcgcagtc gaacaagacg gagaagctcg gcgagaccgg
    15481 gatttcacgg ctcggtcccg cgccgcgggt gggcgataag ccgctcggtc cctcgccgcg
    15541 cccgatcgcg acgccgccgc ccggcgtcac gccgcgctcc tcggccgatg tgctcacgcc
    15601 gtcccccgcg gaggcggtga agccaagccg caccgcgcat gatcgtggcc tcgaagtgcg
    15661 catggcgatc ggtcacgcgg agaccgcgcc gcatgccgat ggcgaaaacc tcgagatcga
    15721 tccgggcatg cgcctgcgcc aatacgagct cattcgcgag ctcggccgcg gcggcatggg
    15781 gcaggtgttc ctcgcgcgcg acgtcaagct ggctcgccgc gtcgccatca agttcctctc
    15841 gacggcatcg cgcgagctga ccgagcggtt cctacgcgag gcgcggacca ctgccgccgt
    15901 gaatcacgaa aacatcgtcg tgatccacga ggtcgacgag cacgcgggca tgccgcacat
    15961 ggtcctcgaa tatctcgagg gcacatcgct gcgcgatctc atgcgcggac gtgcgtactc
    16021 ggcggggcgc gtcgtcgagc tgatcgtccc ggtcgttcgc gcggtggcgc gtgcgcacgc
    16081 cgctggcatc gtccatcgcg atctcaagcc ggagaacatc ttcgtcacga tcgatggtgc
    16141 gatcaaagtg ctcgacttcg gcatcgcgaa ggcgctcgtc gatccagacg ccgtcccgca
    16201 caaggccaat gcgtcggacc tcgagcacct cgcgaaggat gcgctcaccg gcaagagcgc
    16261 gatcatcggc acgccgcagt acatgtcgcc cgagcagttc ggcctcgacg tcgtcgatca
    16321 ccgaagcgac atctgggcgc tcggcatcat gatgttcgag ctccttacgg gtaagcatcc
    16381 gctcgcgccg tttacgatcc attcgctgct caccgccgct gcgaacgtcg acgagcgaat
    16441 gccggccatc ggctcggtcg tgcacgatct gcccgagaag ctcgagcgca tcgtcgaccg
    16501 gtgcctcgcg aagcgcaaag gcgaacgctt ctccgacgcc aagtcgctgc tcgccgagct
    16561 cgagagcctt gttcccggca agctcggtcg caagctccgc gacgacgaga gtccgtatcc
    16621 cggactatcg gcattccagg aagacgatgc cgatcgcttc ttcggacgtg gtcgcgaggt
    16681 catgcggttg cagacgcggt tgcgcgatca cggcttgctc gcaatcgcgg gcccgtcggg
    16741 cgtcggcaag tcgtcgttcg tgcgcgctgg aatcgtgccc gcgctcaagc gcatgggcga
    16801 gacgtgggag acgtacatcc tgcggccggg tcgccagccg atggcgagcc tcgccggtgt
    16861 gctcgcgccg ctgatctcgg cgagcgggga gggcgtccgc acgacgaacc cgaacgcgct
    16921 cgccgattac gaaacgctgg tcgcgcgcct aacggcggag ccgggctacc tcggccagct
    16981 actccgcgaa cgcgccgcgc gcaagaacgc gcacgtgttg ctgttcgtcg atcaattcga
    17041 agagctctac acactcgttc ccgacgaggc acagcgccgc gcgttcaccg cgtgccttgc
    17101 gggtgccgcc gacgacgcga gctcgccgat ccggatcatc tgctcgctgc gatccgactt
    17161 cctcgatcgc gtcgccgagg atcgcgcgtt cctcgatgag ctgatgcgcg gcctcatctt
    17221 cttgcagccg ctcggccgcc ccgagctgcg cgaagcactc tcgcgcccgc tcgacgcgca
    17281 cggctatcgc tatgaatccg acgagctgct cgagcagatg ctcgacgagc tggccgcggt
    17341 tccgggcgca ctgccgctcc tgcagtacgc cggtgctcgg ctgtgggatg cgcgcgatcg
    17401 caagaacaag gtgctcacgg cgtctgcgca cgctgcgatg ggcggcatcg ccggcgccct
    17461 cgcgacccac gcggatcaat tcctcgccgg cctgcccgcc gactcgcatc gcctcgtgcg
    17521 cgcgatcttc cagcggctcg tcacgccgga gcgtacgcgc gcgattgcgg agctcgagga
    17581 tctcgaagca ctcgcgaccg atcccgcaga cacgcgccgc atcatcgatc agctcgtcgc
    17641 cgcgcgcctt ctcgtcgtgc aagtccacgg tgaaaccggt ggagcgagcg tggagatcgt
    17701 gcacgagtcg ctgctcgatg catggccgac gctccgtcgc tggctcgacg aagatcagga
    17761 agacgctgcg ttcctcgcgc agctcgcgac ggcagcgaag cagtgggagt cgaaaggccg
    17821 tgcgcaaggt ctgctctggc gcggcgacgc gatgctcgaa gcgcagcgct gggccaagac
    17881 gcgcgcacgc cgcttgcccg atcgcgatca ggcatacctc gatgccgtcc tcgcgctcgc
    17941 tcgtcgcacg cgccgcgtcc ggttcgccgc tgtcgttgcg gccttcgcgg tgatgacggc
    18001 aatcgccggt ggcgcatcgt tcgcgtacac gcgcgtgcgt gctgctaacg aggtagccga
    18061 gcaccagcgt caggtcgccg agaaggcgct cgtcgacaaa atcgccgagg aaacagcgcg
    18121 ccgccaagct gaggatcagc gcacggccgc gctcgcgtcg ctcctcaccg aagagcagct
    18181 gcgcaaagcg gcggaagacg gtctcatcac cgccgagcag ctcgcgcaaa tcgccgagat
    18241 ccagaagcgc gttgcgcaga agaagctcgt cacgtccgag cagcagcgca atcagctcgc
    18301 cgagcagctg cgcaagtcgg aagcggagaa gcgcgccgca gaggccgctg cgcagcagaa
    18361 gtcagccgag gctcagatga cgcgcgagca gctcgtcgag aagaaccaac agctcgaggc
    18421 cgcgctcagc tccgcaaaga ccgcgcgcga gaaagccgag gcgttgcggg ccgactccga
    18481 tcgcatcagc gcccagctca agcaagcgct cgccgccaaa caggccgaag tcgagcgcct
    18541 cgaaaaagag cgcaagaaga tcacgacgga cctgaagtag cccgccgcga tccgcttact
    18601 tcacgacgac aggaatctcg tgcggcgagt tcgacgcgtt cgcggccgac accgcgacct
    18661 tcacgagacc gcggctcgtt cccgtgaact gaaccgtggc gatgccattg gcgtcggtcg
    18721 tggcttgggt caccgaaagc gtccccgcgc cagcgctgcc cgcggcgatc gcgaatgtga
    18781 cctgcacgcc ggcgacgaca gcgccttggt tcttcgtgcg gatgctgacc gccgaggtct
    18841 gcttctcctg gatcacgcgt ggctcggcgt gcggctccgt gaacacgacg gactgcgaca
    18901 cagccgccgt cgtgaattgg ccctgcgccg tcacgagatc gtccccatcg atcgcgtggc
    18961 ggttgctgac ctggaacttg tacgtcgtgc cgggctgcag agccggcaga tcgagcgcgt
    19021 gcgcgcaaat gttgaagccg tcctgcacgc tttgcgtcag cccgttcgtt ccgtacgcga
    19081 ccttgccgaa gccgggcacg ttcgactgcc acgcgacggt cgcgctgttc gtcgttggcg
    19141 tcgcgctgac cgtgctgacc atctggttgc cgcagaacag cggtggcagt ggtgccgatg
    19201 cgacgaggtc ggcgttggcg agcgtcgggt cagttgcggt gatgcggaag aagtatccgg
    19261 tatcctgctg cagcgccgga ccgaccggca gcgtgacgat ctgctgcgtc gccttgatcg
    19321 gattgccacc ggcatcctcg ctgttcttga tcagaacagg agccccattg ccgtcggacg
    19381 tcgtgaagat ctctaccttg ccgaaccacg cgacatcagt gagccacttg aactccaccg
    19441 agttgttcgc gcttacgcgg accgaaggtc cacccgtgaa ttgcgccatg tccaccctcc
    19501 tagttgtgat cgatattcta gtagagtcag cgcgtggtgg ggctccgcgc gatcggaatc
    19561 gcgatggttg tcgttattgc cggcgcgggc tgcctggcat tcgatgacgt gcagtgcgcc
    19621 gatgggcatc tctgtccgcc cggaagccgt tgcgacgacg ttcacggtgg ctgcatcttg
    19681 ccgtcgcagc tgcaagcttg tgtgggcatc cccgacggtg gcgactgcca gatcgacggc
    19741 gcagacggcg catgtcggaa tggcgcgtgc gtcgcgttca cgtgcggtga cggactgcgc
    19801 gatggcgtcg agccgtgcga aggcgacgac ttcggcggaa tgacctgcac ggatttcggc
    19861 ttcacaaacc cagacggcct cgcgtgcacc gacgactgcc acatcaataa ggacaattgc
    19921 accggcggtt gtggagacaa catcaaggac caaaccgagc tctgcgacgg gacggacatc
    19981 cccgcggcca cgacctgtca gacgctcggc ttctacgatg cggcgggcct cgagtgctcg
    20041 ccgttctgta cgttcgacgt agcgaagtgc actgggttct gcggcgatgg tgcgcagaat
    20101 ggtcccgaac agtgtgatgc gttcgcgcct gccggacaga cctgcctcga ctacagcttc
    20161 gatggcgggc gccttggctg cacgctctgc gcgccgggct tcgagggctg cacgagcttc
    20221 ggctggaagc gaatccagtc gccggcgacg cagcggttgc gcggtatctg gggcacgagc
    20281 tcgagcaact tctatatcgt cggccttgca ggaacgctgc ttcgctataa cggttctcag
    20341 ctctcgacgg tcgttccggc tccgacgacg caagatctca acgctgtatg gggcagcgac
    20401 gcgtcgaaca tcttcgccgt cggtgcgtct ggaacgattc tccgattcga cggctcggca
    20461 tggaccagca cgacctctgg ctcggcgctg ttgctcggcg tctggggctc gagcgcgaac
    20521 gacgtctacg cagtcggctc gggcggaacg gttcttcact atgcaggttc gtgggcaccg
    20581 gtaatgggca cgggcgccgg cacgaacgat cttcgagcag tctggggcat tggctcgcac
    20641 gtcttcgcgg tcggcacgag cggcaccgtg cttcactacg acagcgggac gtggacagcc
    20701 acggcgatcg caggctcacc ggatctattt ggcgtgtggg gcaccagcgc gacgaacgtc
    20761 tacgcgtcgg gcactaccgc tacgtcgaac acagcggtct atcgcttcga cggtttcacg
    20821 tggacgttgg tctatcaagg agggcaaggc gggcgattca acgcggtctg ggcagcatcg
    20881 gcaaccgatg cgttcgcggt tggcacagcc atcgggcact tcgacgggtc gaaatggagc
    20941 ccattgctgc ttccttcgga tgtctccgcg ggcacccggt tgtggggcgt ttggggctca
    21001 ccaacaggtg acgtctgggc gatcggcgat gccggttacg tcgcgcgctg gggcggggcg
    21061 agctggacgg tgccgcaagt gacaccggcg acgaccgtgt cgttccgcgg cgtctcgacg
    21121 tggggctcga gtgacgcgtt tgcggtcggg cttggtgcca ttgctcgcta tgacggcgcg
    21181 agctggacga tcaccaacac cacctcggcg accgatcagc tctacggcgt gtgggcggcg
    21241 agccagagct ttgcagtggc ggtcggtgag acctcgacct cgagcgccgg catcatctat
    21301 cacttcgacg gcaacagctg gatcaacctg ccgtcgggcg actcgcgaaa ctgcggtggt
    21361 ctgccaagtt ttgggctgcg cagcgtgtgg ggctcgagca cgtcgaacat ctacagcgtt
    21421 ggtggcggca cgtccggcat tttccgccgg ttcgccacgt gctggtcagc tccgacggcg
    21481 caaccgtccg gtgccatgca gctgttcgga atccatggca cgagcgcgac gaatgcgttc
    21541 gccgcgagcg tgggcggcct cgtgtatgcg ttcgacggag cgagctggac gtcgctgccg
    21601 acgcctccta cgacgagctt tctgattacg gtgtggacga gcggagcgaa cgatctgttt
    21661 gcagtcggcg atgcaatcct tcactacgac ggcacgaact gggcgcggat gtccgcgaac
    21721 ggccagaacc tcggcggagt ctccggcacg ggaccgcgca acgtgttcgc cgtcggcaac
    21781 aacagccgct tgctccacta cgatggcatc agctggacgc ccatgcgcgt gccgacgtcg
    21841 atcgcatcta cgtatcttcg cagcgtcagc gcgacgcagc gctcgctgct tgtcgtcggc
    21901 gataacggct tcatgcagcg cctcgcattc agtcagcgat cgagtgaagt gaattgccgt
    21961 gatgcctggg acgacgacgc cgatggcatg cccgactgtg cagacactga ttgcgccgcc
    22021 gacgcgtact gcaaacgcgg aggtgcatgc tccgtgatcc gcgacgtcac gtgcgggcaa
    22081 acgatcaccg gttcgacgct cggtgcgacg cccgggcgcg actactacac ctgcgactcg
    22141 aacgccgaga ccggtccaga ggctatctat cggctggtcc cgcccgcgac tgggacggcc
    22201 acagcaacgc tgtcggcgtt tgctccagcg gagctcgatc tcgtggtcgt aggcgcgatg
    22261 ccctcgacgg atgcatgcga tccggatatc aactgcgacg gcgcgagctc gaccgacgga
    22321 acggcgaccg aggcagtgac attcgggacg acgatgaacc ggccggagtt catcatcgtc
    22381 gagggtcgca acgctgcggc aggacccttt acactgaagg tgacgtgcca atgatgcgct
    22441 ggatcctgct cgtgctcgcg ttcacggtcg ccagcgcgca cgcagacaac gagccgtgga
    22501 gagtcggcgt cacgcccgag cgcatgcaag ccgcaaagaa gctgctcgac gagggcaacg
    22561 acctgttgct cgcgcgcgat tggcaggcgg cactggtgaa gtaccaggcc gcgatcgagc
    22621 agtgggacca cccgggcatt cgcttcaata tcgtgaagtg tctcgtccag ctcgatcgca
    22681 cgatcgaagc ggtcgacaac ctcgacctcg cgttgaagta tggcgcggcg ccgctcgacg
    22741 agacggtgta cagcgaagcg ctcggctatc agaagctgct cgcgaatcag gtcgcgacga
    22801 tcgccgtcga gtgcaagcag cccggcgtcg aggtgacgct cgacggccaa ccgctcgatg
    22861 cgtgtccggg aacgagcaag cggcgcgtgc tgcccaatcg ccaccaagtc gtcggcaagc
    22921 gcgccgggtt tctcacccac accgtcgaga tcttcgtgct cggcggcaag accgaggccg
    22981 tgtcggtcgc gctcgatccg gtcgggacgt ccggcacgat ggtgcatcgc tggtcgacgt
    23041 gggtgccgtg ggcggtcgtc ggcgcgggcg tcgtcgcggg cggactcggt gggctctcgc
    23101 aagcgcgcgc ggtcgcgaat cgcgatgcat acgaggacaa gctcgtcggc tgcggcgcgg
    23161 cgggttgccc cgatggcttt cagtcggggc tcagagagcg cgcgacgctg gagaaccggc
    23221 tcgcgatcgg cctgtgggtc acggcgggtg tcgcgatcgt caccggcggc acgatgctct
    23281 acatgaatcg cggccgcacc gtgtacacga agcagaccga gaagatcgag ccgaccgctg
    23341 ccatcacacc cggtggcgcg accgtcggcc tgcgcggctc attctgagtc gtcgtagatc
    23401 gtgatgagct cgtagccgcg cgcgttcgga cgctcggtgt agacgacggc gaggccgcct
    23461 ttgccgccgc tgcacctgat gccgacgacg ccacccttcg cgaggtagcc gttcgcggca
    23521 gtgcgtagcg tctcgtagcc cggcggcgtg ccctcgggca gcggctcgaa ttcttcgccg
    23581 agctggcggc catactcggt catcggtgca gcgccggtgc agcctcccgt gccgaccgtg
    23641 cgctgcgcga ggatcgtgta gaagttgccg acgttgcggc gctcgcgctc gggcgggtag
    23701 ggcgtcccgt cttcggcttc gaagcgtgcg cgaacgtcgt cgggcagatt cggaaaccgg
    23761 ccgttgagat cgtagcgatg gatcaagatc tcgtattggc caacggcgac aaagtcgaac
    23821 aacgagacgt acgcgtcctt gctcgtcttc accgcttgct cgaagtcgcg cgcgaccttc
    23881 tggtgaatcg tggcgggcgc cggcaccatc gggtccgctt tgggcccgca cgccgcgagc
    23941 gcaccggcga ggatcaggct gcgtgcgccc atgcgtcacc gtagtcttcg agtgcactgc
    24001 cgatcggaat gccgagcgct tgaaagcccg gcacgaggac ctcgcgcacg cacgtcgagt
    24061 agatcacgcg ctcttccgct ggcgtcatga ggccatgcgt gcgaaccgcg tccgtcacgt
    24121 caccgatcgg tcgctcgcgc tcgacgacat tcgccacacc gcgcagcgcg atcggcagca
    24181 tctccgccgc cgcgtcgatc acgcccttgc caccgatctc gatggcgtgc cgcatgtacg
    24241 accagccgag gcgcgcgtgc atgacctcgt cggagtagat cgcggtgagc acggcctgcg
    24301 cggtcggatc cgttgtgagg tcgcgcgtcg cggataacac cgtcgtcgcg tacgtctcgg
    24361 agacgcacga cacgagcatg gtgttcgcga gcgcttggtg cagcttcggc cgcgacggat
    24421 cgtcgggcag cgcggacatg cccggcaccg gcaggttctc ttcgccggag tagatcttcg
    24481 ccatgcgcac gcacatctcg gcgtgacgga cttcgtcgat gccagcgcga aggcagagcg
    24541 agatgacgtc ggctggcgct tgcgcggcga cgagatcgat cgcgagcacg gagaacgtcg
    24601 agacggcgag gtactccgcc tcggagcgcc gcatccacgt tcccgcaagg cgcatgcgct
    24661 cggcgggcgt cagcttctcc gcggtggacg catcccacgg aatcggcgtc gagcgccgcc
    24721 gcatcaagcg atggtgttcg gcctcgagcg gcgttcccga cagccaccac gggctcgtcg
    24781 gctcgtaccg ctcgtcgacc ttgtcgtcgt agctgaggac gtcgtcggcc atgcgcggtt
    24841 acgcgagcac gaagccgcga ccgaccggac gaacgtccgg gggcgtgcgc gggcgaggct
    24901 tgcgcaggtt gacgagctcg gtgacgagct tcttgatctg cgcgtccttc gcgtcgatct
    24961 ctttcttgag cttgtcgatt tccttcggct cgaccgaatt cagcttatcg aggagcgtgt
    25021 tgcgctcgct gttcgccgac gagagcgccg ccgccttcac cgtgatcgga tcgggcttcg
    25081 cgggctgcgg cgcggcaacg atctgcgcgg gtgcaccggc agacggcggc gcgggcttcg
    25141 cgggcggatt cgccggctgc gtcgcgacgg gcgcctgctt ctgcggtggg ggcggcgcgg
    25201 cggcctgttc ggcgactgca gagcccggcg tcccgagcat cgcgcccgcg aacacgacgg
    25261 tgcgccagat gcgcgacgaa tacagctcct tcgcaagctc tcgatgctta cccatgatcc
    25321 ctccggtcac ctacgatgac accgggccca ggcgaattcc agagctgcgc gcccatcgca
    25381 gacgcgtggg gcactttgcc tcgcgctacg cgaggacgaa gccgcggcct gtcggacgct
    25441 tgcccgtacc gcgcgcgcgc ttctgcgcag cagcttcctc ggcagcagcc ttctcggccg
    25501 cttctttgtc cgcctgctct tgagcgaggc gcgcttcttc gtccttcttc gcttggtctg
    25561 cagccgcagc gtcggcggcg gccttgtcgg cggcttcctt gtccgcggcg gccttcgctg
    25621 catcggcctg ctgttgcgcc gcttgatcct gcgcagcttg atcctgcgct gccgtatcgc
    25681 cgccacccgc tggcgtcgtg gtcgcgggct tcgagccgcc accgcacgcg gtcgtcgcga
    25741 gcggagcacc gagcatcgcg cctgcgaaca cgacggtgcg ccaaatgcgt gacgcgctcg
    25801 tgcggccaag gctcgacgcg tacagctcct tcgctagatc acgatgattc gccatgcgaa
    25861 cgacgatgac accggcgcta actgatttcc agcggccagc ggcgaaaccg ggcgtgagct
    25921 gcgcgcgaca ccgcgagcgg gtgccagttg cactacttct gcgccatctt cgtcgtgacg
    25981 tcgcgcaaac ggcgcacgag ctcggcgacg atcgcctcgg acatctccgg ccgctcggtc
    26041 atcaggcgct tgaagccttc gcccggaacg cgaagcgcgc gcgtgcgttc gagcgagcga
    26101 acggtggccg agcgcggcga cgcgtcgagc accgccatct cgccgatgca cgcgcccgga
    26161 ccgagctcgt tgatcgtcgt ttcggggcgg ccgtccgtgc caccgacgag cacgcggact
    26221 ttgcccttca cgatcaggta gaccgcatcg ccagcatcgc cctggcggaa gaggtccttg
    26281 tcgcccatga cgatccgctc ttctacgatg tgcgcgagct cttcgaggtc gtcggcgtcg
    26341 agctcggtga agatcgggac ttgccgcagc agcatcatct tctcgatcgt cgtcagcgat
    26401 gacatcgccg cgttggcgtc gagcgtggcg tacgcgcctg ctttgccgac gcccatcgtc
    26461 gtctgtgcga gcttcgtgat gtcgacgacg ggaatctcgc ccgacgtcgt gccggagacc
    26521 gcgcgcaccg cggtgtggcg aaccgtggcg tctggatcgt tcagcacctt tgcgagcagc
    26581 tcgcgcgtca gccggctctt cgcgcccatc gcgcggaccg cggcttcgcg aacgatcgcg
    26641 tcgacatcgt ctagcatctc gagcagggtc gcgcgcgcgc cggcatcctc gaagcgcgag
    26701 agcagcatcg cgacggcggc gcgcagatgc ggagaggcgt cacgcgcgac ggcgagcagc
    26761 ggctctgcct tgaagtcgcc gcggtcttcg cggacgagac gcgtcagcag atcgacgagc
    26821 ggcttgcgca gcgattcgtc gacgggagcg agctcgacga gccgcgatgg atcgatgagg
    26881 ttgcgcgcct tcgcgtgttg ccgcaccgat tcgacttcgg ggcgcttcgt catgacgccg
    26941 aggacaccca gcgcgtggac tgcatgctcg tgcgcgcgcc gctgcgcgag gtggcggagc
    27001 agggcgagcg aggccgaatc gctgttcgcc gcgtccgcgg gtgtggcaca tgcttcggcg
    27061 acggtgacga gccgctccat ctccgcgagc tcaccacgat agatcgccgc gatcgggccg
    27121 ccgggcgcga ggagctcgtc gagcgcggcc ttctcggcgt cggcgtcgac gccgcgttcg
    27181 ttgcgcagcg cttcgagcgt gctgtctgcg ggcgcggagc cggcgcgtgc cgcctcgacg
    27241 acgatgcgct cgaggtcctt gcggtactgc tgcttcgcga gccgctgctc gaccgagcct
    27301 tggtacgccg gatcgaatag ctgacgctct tcggccgaga gctcgccgag gatcttctgg
    27361 tggtctttgt cgctgacgcc aagctgtgcg cgcagtgaat cgaggatgac gagctcgccg
    27421 cgcgtgacga gaccgtccgc caccatttcg cgcaccgtct ctttgtaggc gcgcagtcga
    27481 gcttcgcgct gcttcgtgcg ctcggtgtgg agcaggtaga tgtccttgag atcgtcggac
    27541 ggtggcgcgt ccccccattc ccacttcttc aggatcttct gcgcgaactt ctcctgtacg
    27601 tattggtctt cgcgtcgcga gatgcggcgc atgaagatcg tcgtcgaggc gaacacgacg
    27661 atgaggcccc accccgtgac gacccacgcg ggtagcttct gcagcgtcgg ctggccagcg
    27721 aagcagtaga acgcgttg
//
LOCUS       DATLBZ010000373        11411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9380263, whole genome shotgun sequence.
ACCESSION   DATLBZ010000373 DATLBZ010000000
VERSION     DATLBZ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9380263"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VMZ53_19130"
     CDS             <1..725
                     /locus_tag="VMZ53_19130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HUS30635.1"
                     /translation="LFPEGEKFFVESVKQQREKVTDAALLRDIAGFIGQEAMHGKEHR
                     AFNELLVAHGFAEAPRIEKRLRAFLKSVRRVLSPMSQLAVTCALEHFTAMLAEALLRD
                     SRMRDEVHESVRPLWLWHALEESEHKAVAFDVYKAAGGRYPRRVLIMLLTTAVFFAAQ
                     AIVHARLMATRGILGKPWTWVRGATRFWIYPGFLTRLTPAYLSYFRPGFHPNDRDTNE
                     LLDSWRISLFGADGIVALRRVS"
     gene            750..2633
                     /locus_tag="VMZ53_19135"
     CDS             750..2633
                     /locus_tag="VMZ53_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30636.1"
                     /translation="MAGRTHAQQLANHTLLQQVSADPLSLTRAIAAGEGERVLQEIWQ
                     KAAYELAPIARMPGVGLSATMERHGLYTVVQVMPPPPRESGDPAAIVIIGRGDGSSKL
                     SGIGYYVLSLVLDAVTGAPRFTFASRDARNPKAVVTVEGPLPDPAWLMEYASALYEGI
                     KPEPRNRQAEVPTLPSWYWWYAFEARDAMKFFADAETDPDRFDAVRRFPILLMPEIAD
                     AGEALVGEKPAKKLRELRPYLRRDPSMAPAWKGLAERLANTTGGAPGAHTTRAIELLA
                     EPREHGALTKPAAYELEAFVRCKLALLGIEPDENNRIAQELAVAAARDSQPIIMPGGK
                     SHEPAEDPLWLKLFLDDTDLPNWTLSTFDEEFANADPTFLHFGGARAAFVVWSADESS
                     AMAHLMDTRWVFRTNFGAQSYLREPATLTMLADGVPPVPAPELGDEAIAFGGTAPDGR
                     RTQILVIRIGRVLARIQANEGINATAARQILHAAVLHPLGIRVAQRGREGIAAYWLNV
                     AAPTNAVPALLHSPGYDVARLIEKYPLLAHSELPRAIATLGGKYEPVARSLASYQAQL
                     RAHRWQTYRSAMLALVKALVETDMGDTRVNAAFALEIVYELGHIDPDPVWRQLAVTCE
                     ARL"
     gene            complement(2630..3562)
                     /locus_tag="VMZ53_19140"
     CDS             complement(2630..3562)
                     /locus_tag="VMZ53_19140"
                     /inference="COORDINATES: protein motif:HMM:NF042890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6748 domain-containing protein"
                     /protein_id="HUS30637.1"
                     /translation="MTSRILFALACTLPLTACLDSSSSTDELDQDDATAGDASKADAA
                     GGTYTYYFVQQDQRRCASPMCGGVFYRLANATKTVCLDGKKADRCYAASSDLTKLGLD
                     ETNTTKVQDKLNYGEVLMRATIGSKAYPGVGTFAKLNAKEAWLPQGPNPVEGPIMKIE
                     DSGIRCITFPCPSLNEKKLNSSVTQAIADLQWDHSGASERLVGTALDKLHTDGLIVSG
                     YRTTVTGPAGQGKARSVTQLWFKQTNEPAAAKCFVGGCSGQVCSDDPNPFTTCEFQPE
                     YACYHTATCAVQADGNCGWTQTAELSACLADPPN"
     gene            complement(3712..4770)
                     /locus_tag="VMZ53_19145"
     CDS             complement(3712..4770)
                     /locus_tag="VMZ53_19145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30638.1"
                     /translation="MPRDADTDPDRTKVSIVDPLVGTVLDRRYRIEFRLAAGGFGAIY
                     RATHVITRREVAIKVLHPSLASEPNVVARFRREAEALGALKNSHTVRAFDFGETDGML
                     YIVMELLQGESLYERYRALGPLPWRRMLKIACEVCESLAEAHALGIVHRDLKPTNIHL
                     ERIGGDDDYVKILDFGIAKILHGSALDNADLTRSGQMIGTFDYMAPEQMVGSHLTGQS
                     DIYTLGLVIYEMLTGERPFGDPPSPSAMLQAMIMSTPPSVSSRSDAPEEIDAIVVKCL
                     ARDPDHRWRSVEELRAEIERVLENAGDPTQTQLTRAPSGPFTAPTMDAVEPDDTEIGV
                     DKFKTTLPGVMMPPRRKR"
     gene            4791..6020
                     /locus_tag="VMZ53_19150"
     CDS             4791..6020
                     /locus_tag="VMZ53_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30639.1"
                     /translation="MPVRRHHTDILIAALALVGVTGCGRFGFGDDAPIDAAPDVMPDT
                     PVAIEPMELAITQTFVTAQTTFVDIPAATLTLPASPGTSWLVLTSAKLASPTFSAQSV
                     EARYLLDDVELGMGGTQTSTPGFPGPWQHFVVIAGTTAPQQLRYQLRDVSAAGAILEA
                     LTVEAIPFAAADVAYASNDPVHDVTAVAATADSVLSLGPLSGDYVFFLVANTTDLPGE
                     SDCYVQWMGPNGATWMRDSQQPRESWQSFMTVQRGSFDIVAAHVTLLTHVGSGPCQVR
                     NVRAAAIPTSAFASVDYARNDDATLVNATATPATTVTLTPMPSSATQFLFWGNAMLDE
                     DCLTVADAQRTVVFTHGSTTESISHATDNCSYTATYGHVALLPSAPSSMSIGFSSGSG
                     AGGVDHYASELMLLGLR"
     gene            6037..9378
                     /locus_tag="VMZ53_19155"
     CDS             6037..9378
                     /locus_tag="VMZ53_19155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS30640.1"
                     /translation="MRGHSIGVESVDILTRVLRAEFPELDAERLRGAVEKAVARVASA
                     SLDTEGYAVVDLHLQKVEATEESEERSKILRELSQNLEERGDADRALVTRLSAFSELP
                     TPGDVFPLLRLARLTDRWSELPLDMMSALIDINNDDGVKQLSALADAWQHVGRPYYAA
                     DCLERVLVVDTGNTKAFEALEVFYRTTGEWPVLVDLLGRRAVHVESDKERGELLREMA
                     IIYDKELGDSGAALDAYREADRLVPNMPEVLEGIARLSLEVGVPEDEALSAAERFGSS
                     VVDPKERARALVKAADIAKLQNWDKAQELFEQARKADPELPSAVDGLATLLRDKGQLS
                     EAITLLVNAAERSKTDRARWLADAADYCVALGDTDWAKQLYRDARAADPTNMKAGIAM
                     VELGWDSGGHADAGELIPILDQLCKTTDDPSRLRGYLIQRSKLALEVGERTQARNLLA
                     RAVELDPEDIEPRRDLAQMLFDAGQWVKARQVMETLLVDEDLLPRDVAIELHYRVARC
                     ARELGDVEGAQKHVDIALVLQPDHRPALLLRTELGQADPHQLVADQLALASTAPPEER
                     ATRFAAIGDRYMELGDRPAAREMYREAIQHRPGDHLLLTKFLELVADDGDWSYSLDVV
                     RKLIGSEKDPKVRARYKHLAGMIARDELEDHEQAAELFVSAIEDDVFSFAAADELETL
                     LEHSDDRPALAAFYYKRLEHMREREGRPGERLRLWDHLGELLMELERRDDAVMAFEVA
                     SSLDPDNVERKKRLADLYTLDAKHDAKAIATHQAILKADKKRVESYKALRDLYERTRQ
                     PERARAVAEALELLASKDDRIGSLFDGKPLDAAPRATVPIRPFASEDWLDLTRLDVDL
                     TLSALFAVVAPPFAIERARMRPPAAVSSKEHDVPPHVQKVLLEVSRIFGISPRPPSYF
                     EREQAAPCKMAMRTRDGTLVPVLVFGRTVMDKAISDHELTFSLARAMCDLRTERIARL
                     LCPRAGELSQIIELAMGSEADSSSAAKWLTAALHPVELQQARGIGARLRDRKLHPMTA
                     AVGWLAATERAADRVGLVISGDLAKCARVIEQDTITAADSSRIVELAWASTTDEVLGV
                     RARVEGWAAVPPPIPPRVTEARRST"
     gene            9393..10469
                     /locus_tag="VMZ53_19160"
     CDS             9393..10469
                     /locus_tag="VMZ53_19160"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HUS30641.1"
                     /translation="MEVFDRLSKYDFGEVHFKLDKSTHLQAIVAIHDSRLGPALGGCR
                     FIEYPSDEAAIEDALRLARGMTYKAALAGLAHGGGKSVIMRPKRHFDRVALFRAFGRF
                     IEDLRGHYITAEDSGTGLEDMEIIRTQTKNVTGIDPAHGGSGDPSPFTALGVRRGIEA
                     CVKFKLGKDSLQGIHVAVQGVGHVGYYLCKELHAAGAKLTVADVDKLKSERAKREFGA
                     AIVNIDDVAKVDCDVFAPCALGAGLNDDTIPHLHAPIVAGAANNQLLEPRHGDDLHAR
                     GILYAPDYAINAGGLVNVAQEVKGYDAKVAREKTLLIYNTIYDICERSKKLGAPTYRV
                     ADIIVEEKLAAAPKRQSSIAPPKA"
     gene            complement(10577..11110)
                     /locus_tag="VMZ53_19165"
     CDS             complement(10577..11110)
                     /locus_tag="VMZ53_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30642.1"
                     /translation="MSTSRLLSVSVVAAVTVGATMFACGGDDGGTHSHGDPDAKVFMD
                     AKVFMDAPGTGSNNTVGLGQACTPPAQGSGQGDCPTGYICLNLTGASHPWCSKPCNTA
                     SDTCAQGYTGPGVAGCIEQITFNGGAPMDFCGVTCAGDGVNGCTAATCTGMCPGQMTC
                     TGTLLNGSGTAVGSACN"
BASE COUNT         1943 a         3791 c         3754 g         1923 t
ORIGIN      
        1 tgctgtttcc ggaaggcgag aagttcttcg tcgagagcgt gaagcagcag cgcgagaagg
       61 tgacggacgc cgcgctgctt cgtgacatcg ccggcttcat cgggcaggag gcgatgcatg
      121 gcaaagagca ccgcgcgttc aacgagctgc tcgtcgcgca tggcttcgcg gaggcgccgc
      181 gcatcgagaa gcggctgcgt gcgttcttga agagcgtgcg gcgcgtgctc tcgccgatga
      241 gccagctcgc tgtgacgtgc gcactcgagc acttcacggc gatgttggcg gaagcgttgc
      301 tgcgcgattc gcgcatgcga gatgaagtgc acgagagcgt gcggccactg tggctgtggc
      361 atgcgctcga agagagcgag cacaaggccg tggcgttcga tgtgtacaag gcagcgggcg
      421 ggcgctatcc gcggcgcgtg ctgatcatgc tgctcacgac ggcggtcttc tttgctgcgc
      481 aagcgatcgt ccacgcgcgc ctcatggcga cgcgcgggat tctgggcaag ccgtggacgt
      541 gggtgcgtgg tgcgacgcgg ttctggattt atccggggtt cttgacgcgc cttacgcctg
      601 cgtacctctc gtacttccgt cctgggtttc atcccaacga tcgggatacg aacgagctgt
      661 tggattcctg gcgaatttcg ctatttggtg cggatgggat cgtcgcgctg cgccgagtga
      721 gctgatcgct cgtacaatag ccagaccgga tggctggccg tacgcacgcg cagcagctcg
      781 cgaaccacac gcttctgcag caggtctctg cggatccact gtcgctgacg cgcgcgatcg
      841 cagctggcga gggcgagcgc gtgttgcagg agatctggca gaaggcggcg tacgagctcg
      901 cgccgatcgc gcgcatgcct ggagtcgggc tctccgcgac gatggagcga cacggcctgt
      961 acaccgtcgt gcaggtgatg ccgccgccgc cacgcgagtc cggagacccg gctgcgatcg
     1021 taatcatcgg gcgcggcgat ggatcgtcga agctgtcggg catcggctac tacgtgctgt
     1081 cgctcgtgct cgatgcggtg accggcgcgc cacgcttcac gtttgcgtcg cgcgatgcgc
     1141 gcaatccgaa ggcagttgtc acggtcgaag gaccgctgcc cgatccggcg tggctcatgg
     1201 agtacgcgtc ggcgctgtac gagggcatca agcccgagcc gcgcaaccgc caggcagaag
     1261 ttccgacgct gccatcttgg tactggtggt acgcgttcga ggcgcgcgac gcgatgaagt
     1321 tcttcgccga tgcggagacc gatccggatc gcttcgatgc ggtgcgccgc tttcccattc
     1381 tgctgatgcc cgagattgca gatgctggcg aagcgctcgt cggcgagaag cccgcgaaga
     1441 agctgcgcga gttgcggccg tacctgcggc gcgatccatc gatggcacct gcgtggaagg
     1501 gcctcgccga gaggctagcg aacacgacgg gcggcgctcc gggtgcgcac acgacgcgcg
     1561 cgatcgaatt gctcgccgag ccgcgcgagc acggcgcgtt gacgaagcct gcggcgtacg
     1621 agctcgaagc gtttgtccgc tgcaagctcg cgctgctcgg catcgagccg gacgagaaca
     1681 atcgcatcgc ccaggagctg gcggtggccg cggcgcgcga ctcgcaaccg atcatcatgc
     1741 ccggtggcaa atcgcacgag ccggccgagg acccgctgtg gctcaagctc ttcctcgacg
     1801 acaccgactt gccgaactgg acgctgtcga cctttgacga agagttcgcg aacgccgatc
     1861 cgacgtttct acatttcggc ggcgcgcgcg cagcgttcgt ggtctggagc gccgacgaga
     1921 gcagcgcgat ggcgcacctc atggacacgc gctgggtgtt ccgcacgaac ttcggcgcgc
     1981 agagctacct gcgcgagccc gcgacgctca cgatgctcgc cgacggcgtg ccacccgtgc
     2041 ccgcgcccga gctcggcgac gaggcgatcg cattcggcgg gaccgcgccc gacggccggc
     2101 gcacgcagat cctcgtcatt cgcatcggcc gcgtgctcgc gcgcatccag gcgaacgaag
     2161 gcatcaacgc gacggccgcg cgccaaatcc tccacgccgc ggtgttgcat ccactcggca
     2221 ttcgcgtcgc gcagcggggc cgggagggca tcgcagcgta ctggctcaac gtcgccgcac
     2281 cgacgaacgc cgtccctgcc ctgctccact cacccggcta cgacgtcgcg cggctgatcg
     2341 aaaagtatcc actcctcgcg cacagcgagc tgcctcgcgc gatcgccacg ctcggcggca
     2401 agtacgagcc tgtcgctcga tcgctcgcga gctatcaagc gcagctgcgc gcgcatcgct
     2461 ggcagacgta tcgctccgcc atgctcgcgc tcgtgaaggc gctcgtcgaa acagatatgg
     2521 gcgacacgcg cgtgaatgca gcgttcgccc tcgagatcgt ctacgagctg ggccacatcg
     2581 atccagatcc ggtgtggcga cagctcgctg ttacatgtga agcgcggctc tagttcggcg
     2641 ggtccgcgag gcacgccgag agctcggcgg tctgcgtcca accgcagtta ccgtccgcct
     2701 gcactgcgca ggtcgccgtg tgatagcagg cgtactccgg ctggaactcg cacgtcgtga
     2761 acgggttcgg atcgtcgctg cagacctgac ccgagcaacc accgacgaag cacttcgccg
     2821 cggcgggctc gttcgtctgc ttgaaccaga gctgcgtcac gctgcgcgcc ttgccttgac
     2881 cggccgggcc cgtcaccgtc gtgcggtagc ccgagacgat cagaccatcg gtgtgcagct
     2941 tgtcgagcgc ggtgccgacg aggcgctcgc tcgcgcccga gtgatcccac tgcagatccg
     3001 cgatcgcttg cgtgaccgag ctgttgagct tcttctcgtt gagcgacggg cacgggaacg
     3061 tgatgcaacg gatgcccgag tcctcgatct tcatgatcgg gccttcgacc gggttcggtc
     3121 cctgcggcag ccacgcttct ttcgcgttga gcttcgcgaa cgtgccgacg cccgggtacg
     3181 ccttcgaacc gatcgtcgcg cgcatcagga cttcgccgta gttgagcttg tcctgaacct
     3241 tggtggtgtt ggtctcgtcg agaccgagct tcgtgagatc cgagctcgcg gcgtagcagc
     3301 ggtcggcctt cttgccgtcg aggcaaacgg tcttcgtcgc gttcgcgagg cgatagaaga
     3361 cgccaccgca catcggcgag gcgcagcgac gctggtcctg ctggacgaag taatacgtgt
     3421 acgttccgcc cgctgcgtcg gccttggagg cgtcgccggc ggtggcgtcg tcctggtcga
     3481 gctcatcggt cgaagacgac gagtcgagac aggcggtgag cgggagggtg caagcgaggg
     3541 caaatagaat tcggctagtc attggaaata ggtcctttag atgaccgccg cctggagcat
     3601 tctccatgcc acaaagcgtc attgcgcgac gaggagttgg cgctgcgcgg acttgggttc
     3661 actagcccag tagccactcg agtggccacc ccggtggccg cgacagatgc gtcagcgttt
     3721 gcgacgcggc ggcatcatca cgccgggcag cgtggtcttg aacttgtcga cgccgatttc
     3781 ggtgtcatcg ggctcgacgg cgtccatcgt cggcgctgtg aacggaccgc tcggtgcgcg
     3841 cgtgagctgt gtctgcgttg gatcgccggc gttctcgagc acgcgttcga tctcggcgcg
     3901 aagctcttcg acgctgcgcc accggtgatc cggatcgcgc gcaagacact tcacgacaat
     3961 cgcgtcgatc tcctcgggag catcactgcg cgacgacacc gacggaggcg tcgacatgat
     4021 catcgcctgc agcatcgctg atggtgacgg cggatcgccg aacggtcttt caccggtgag
     4081 catctcgtag atgacgagac cgagcgtgta gatatcgctc tgcccggtga ggtgacttcc
     4141 gaccatttgc tccggcgcca tgtaatcgaa cgtgccgatc atctggccgg agcgcgtgag
     4201 gtccgcgttg tcgagcgcgc tgccgtgcag gatcttcgcg atgccgaaat cgaggatctt
     4261 tacgtagtcg tcatcaccgc cgatacgttc gagatgaatg ttggtcggct tgagatcgcg
     4321 atggacgatg ccgagcgcgt gcgcctcggc gagcgattca cacacctcgc acgcgatctt
     4381 caacatgcgc cgccacggca gcggaccgag cgcgcgatac cgctcgtaca ggctctcgcc
     4441 ctgcagaagc tccatcacga tgtagagcat gccgtcggtc tcgccgaaat cgaacgcgcg
     4501 gacggtgtgt gaattcttca gcgcgccgag tgcttctgct tcgcgccgaa accgcgcgac
     4561 gacgttcggc tcgctcgcga gcgatggatg caggactttg atcgcgacct cgcggcgcgt
     4621 gatcacgtgc gtcgcgcgat agatcgcacc gaagccaccg gcagcgagac gaaactcgat
     4681 gcgatagcga cgatcgagca cggtgccgac gagcggatcg acgatcgaga ccttcgtgcg
     4741 gtctggatcg gtatcggcgt cgcgcggcat gagcgcggta tagtggcatc gtgcccgtcc
     4801 gccgtcacca cacggacatc ctgatcgcag cgctcgcgct cgtcggcgtg accggttgcg
     4861 ggcgcttcgg tttcggagac gacgcaccga tcgacgccgc acccgatgtc atgccggata
     4921 cacccgtcgc gatcgagccg atggagctcg cgatcacgca gacgttcgtc acggcacaga
     4981 ccacgttcgt cgacatcccc gctgcgacgc tgactcttcc cgcttcgccc ggcacatcgt
     5041 ggctcgtgct gacatcggcg aagctcgcct ccccgacgtt ctccgcacag agcgtcgagg
     5101 cgcgctatct cctcgatgac gtcgagctcg gcatgggcgg cacgcagacg agcacgcctg
     5161 gctttcccgg accgtggcaa cacttcgtcg tcatcgccgg cacgaccgcg ccgcagcagc
     5221 tgcgctacca actgcgggac gtgagtgcgg caggagcgat tctcgaagcg ctcacggtcg
     5281 aagcgattcc gttcgcggcg gccgatgtcg cgtacgcgtc gaacgatcca gtccacgacg
     5341 tcaccgcagt agctgcgacc gccgatagcg tgctctcgct cggcccgctc agcggcgact
     5401 acgtattctt cctcgtcgcc aacacgacgg acctgccggg tgagtcggac tgctacgtcc
     5461 agtggatggg cccgaacggt gcgacctgga tgcgcgactc gcagcagcca cgcgaatcgt
     5521 ggcaatcgtt catgacggtg cagcgcggct cgttcgacat cgtagctgcg cacgtcacgc
     5581 tgctgacgca tgtcggaagc ggaccgtgtc aggtgcgcaa cgtgcgtgcc gcggcgattc
     5641 cgacgagcgc gtttgcgtcg gtcgactacg cgcgcaacga cgatgcaacg ctcgtgaacg
     5701 caaccgcgac gccagcgacg acagtgacgc ttaccccgat gccgtccagc gcgacgcagt
     5761 ttctgttctg gggcaacgcg atgctcgacg aggattgcct gacggtcgcc gacgctcagc
     5821 gcaccgtggt gttcacgcac ggctcgacaa cggagtccat ctcgcacgcg accgacaact
     5881 gctcgtacac ggcgacgtac ggacatgttg cgctgttgcc gtcggcaccg tcgtcgatga
     5941 gcattggctt cagctcggga agcggcgcag gtggcgtcga ccactatgcg tccgagctga
     6001 tgctgctcgg cctgcgctga tgttacgctc gcgctcatgc ggggtcactc catcggtgtc
     6061 gagtccgtcg acatcctcac gcgagttctg cgtgccgagt tccccgagtt agacgccgag
     6121 cggctgcgcg gcgcggtcga aaaggcagtc gcgcgagtcg cgagcgcatc gctcgatacc
     6181 gaaggctacg ccgtcgtcga tctgcatctg cagaaggtcg aggccaccga ggagagcgaa
     6241 gagcgctcga agatcttgcg cgagctgtcg cagaacctcg aggagcgagg cgatgccgat
     6301 cgcgcgctcg tcacgcggct ctccgcgttc tcggagctgc cgacgcctgg tgacgtgttt
     6361 ccgctcttgc gcctcgcgcg cctcaccgat cgctggtccg agctgccgct cgatatgatg
     6421 agcgcgctca tcgacatcaa caacgacgat ggcgtgaagc agctctcagc gctcgccgac
     6481 gcgtggcagc acgtcggtcg tccgtactac gcagccgatt gcctcgagcg cgtgctcgtc
     6541 gtcgataccg gcaatacgaa ggcgtttgaa gcgctcgaag tgttctatcg aacgaccggt
     6601 gagtggccgg tgctcgtcga tctcctcggc cgtcgcgccg tgcacgtcga gagcgacaaa
     6661 gagcgcggcg agctgctgcg cgagatggcg atcatctacg acaaggagct cggcgacagt
     6721 ggcgcggcgc tcgatgcgta tcgcgaagcg gatcggctcg tgccgaacat gcccgaggtg
     6781 ctcgaaggca tcgcgcggct gtcgctcgag gttggcgtcc ccgaagacga ggcgttgtcc
     6841 gcggccgaac gatttggcag ctccgtcgtc gatccgaagg agcgcgcgcg cgccctcgtg
     6901 aaagcggccg acatcgcgaa gctgcagaat tgggacaagg cgcaggagct gttcgagcag
     6961 gcgcgcaagg cagatccaga gttgccgtct gccgtcgatg gtctcgcgac gctgctgcgc
     7021 gacaaaggtc agctctccga ggcgatcacg ctgctcgtca atgcggcgga gcgcagcaag
     7081 accgatcgcg cgcggtggct cgccgatgcg gctgactact gcgtcgccct cggcgatacc
     7141 gactgggcga agcagctcta tcgcgatgcg cgcgcagcgg atccgacgaa catgaaggcc
     7201 ggcatcgcga tggtcgagct cggctgggac agcggcggcc acgccgatgc gggcgagctg
     7261 attccaatcc tcgatcagct ctgcaagacc accgatgatc cgagccgcct gcgcggctac
     7321 ctcatccagc gcagcaagct cgcgctcgag gtcggcgagc gaacgcaggc gcgcaacttg
     7381 ctcgcacgcg ccgtcgagct cgatccggaa gacatcgagc cgcgccgcga cctcgcgcag
     7441 atgctgttcg acgccggcca gtgggtgaag gcgcgccagg tgatggagac gctgctcgtc
     7501 gacgaagacc tgctgccgcg cgacgtcgcg atcgagctgc actatcgagt ggcgcgatgc
     7561 gcgcgcgagc tcggcgatgt cgagggcgcg cagaagcacg tcgatatcgc gctcgtgctg
     7621 caacccgacc atcgtccagc gctgctgctg cgcaccgagc tcggtcaggc cgatccgcac
     7681 cagctcgtcg ccgatcagct cgcgctcgcg tcgaccgcgc cgccggaaga gcgcgccaca
     7741 cggttcgcgg cgatcggcga tcgctacatg gagctcggcg atcggcccgc cgcgcgcgag
     7801 atgtaccgcg aggcgatcca gcatcggcct ggcgatcact tgctgctgac gaagttcctc
     7861 gagctcgttg ccgacgatgg tgactggagc tactcgctcg acgtcgtgcg caagctgatc
     7921 ggcagcgaga aggatccgaa ggtccgcgcg cgctacaagc acctcgccgg catgatcgcg
     7981 cgcgacgagc tcgaggatca cgagcaagca gccgagctgt tcgtctctgc gatcgaagat
     8041 gacgtgttct cgtttgccgc cgccgacgag ctcgagacgc tgctcgagca ctccgacgat
     8101 cgcccggcgc tcgccgcgtt ctattacaag cgcctcgagc acatgcgcga gcgcgaaggt
     8161 cgccccggtg agcggctgcg cctgtgggac cacctcggcg agctgctgat ggagctcgag
     8221 cgtcgcgatg atgccgtcat ggcgttcgag gtcgcgtcgt cgctcgatcc ggacaacgtc
     8281 gagcgcaaga agcggctcgc ggatctgtac acgctcgacg ccaagcacga cgcaaaggcg
     8341 atcgcgacgc accaagcgat cctcaaagcc gacaagaagc gcgtcgagtc gtacaaagcg
     8401 ctgcgcgacc tctacgagcg aacgcgccag ccggagcgcg cacgcgccgt cgccgaagcg
     8461 ctcgagctgt tggcgtcgaa agacgatcgc atcggctcgc tgttcgacgg caagccgctc
     8521 gacgcggccc cgcgcgccac cgtgccgatt cggccattcg cgagcgagga ctggctcgat
     8581 ctcacgcggc tggacgtcga cctgacgctg tcggcactgt tcgcggtcgt cgcgccgccg
     8641 ttcgcgatcg agcgcgcacg catgcggccg cccgcggcgg tgtcgtcgaa ggagcatgac
     8701 gtgccgccgc acgtgcagaa ggtgctgctc gaggtctcgc gcatctttgg catcagcccg
     8761 cgcccaccgt cgtacttcga gcgcgagcaa gcggccccgt gcaagatggc gatgcgcacg
     8821 cgcgatggca cgctcgttcc ggtgctcgtg ttcggccgca cggtgatgga caaggccatc
     8881 tccgatcacg agctgacgtt ctcgctcgca cgcgcgatgt gcgatctgcg caccgaacgc
     8941 atcgcgcgcc tgctctgccc tcgcgccggc gagctctcgc agatcatcga gctggcgatg
     9001 ggctcggaag ccgattcgtc gagcgcggca aagtggctca ccgctgcact ccaccccgtc
     9061 gagctacaac aagcgcgcgg catcggcgca cgccttcgcg atcgcaagct gcacccgatg
     9121 accgccgctg tcggttggct agccgccacc gagcgcgctg ccgatcgcgt gggcctcgtt
     9181 atctcgggcg atctcgcaaa atgcgcacgc gtgatcgagc aagacacgat cacggccgcc
     9241 gactcctcgc gcattgtcga gctcgcgtgg gcatcgacga ccgatgaggt cctcggtgtg
     9301 cgcgcacgcg tcgaaggctg ggccgctgta ccgccgccga ttccgccacg tgtaacggaa
     9361 gctcgtcgga gtacgtgata aaccccaggt cgatggaagt tttcgaccgc ctctccaagt
     9421 acgacttcgg cgaagttcat ttcaagctcg acaagtcgac gcacctgcag gcaatcgtcg
     9481 cgatccacga ctcgcggctg ggtcccgcac tcggtggctg tcgcttcatc gagtatccga
     9541 gcgacgaagc cgcgatcgaa gacgcgctcc gcctcgcacg cggcatgacg tacaaggcgg
     9601 cgctcgccgg cctcgcacac ggcggcggca agtccgtcat catgcgcccg aagcgccact
     9661 tcgatcgcgt ggccctgttc cgcgcgttcg gccgcttcat cgaggacctc cgcggtcact
     9721 acatcaccgc ggaagactcg ggcacgggcc tcgaggacat ggagatcatt cgcacgcaga
     9781 cgaagaacgt cacgggcatc gaccccgcgc acggcggctc gggcgatccg tcgccgttca
     9841 ccgcgctcgg cgttcgtcgc ggcatcgaag cctgtgtcaa attcaagctc ggcaaagact
     9901 cgctccaagg catccacgtc gcggtccaag gcgtcggcca cgtcggctac tacctctgca
     9961 aagagctgca cgccgcaggc gcgaagctca cggtcgccga cgtcgacaag ctgaagtccg
    10021 agcgcgccaa gcgtgagttc ggcgcagcga tcgtcaacat cgacgacgtc gcgaaagtcg
    10081 actgcgacgt gttcgcgcca tgcgccctcg gcgcgggcct caacgacgac acgatcccgc
    10141 acctccacgc gccgatcgtc gcaggtgccg cgaacaacca gctgctcgag ccgcgccacg
    10201 gcgacgacct ccacgcgcgc ggcatcctct acgcgcccga ctacgcgatc aacgcaggcg
    10261 gtctcgttaa cgtcgcgcaa gaagtgaagg gctacgacgc caaggtcgcg cgcgagaaga
    10321 cgctgctgat ctacaacacg atttacgaca tctgcgagcg cagcaagaag ctgggcgcgc
    10381 cgacgtaccg cgtcgcagac atcatcgtcg aagagaagct cgccgccgct ccgaagcgcc
    10441 agtcgtcgat cgcgcctccg aaggcgtagg gctcgtcgcg agacgctcgc gtcgccgcgg
    10501 cttgcctcgt cgggcgaagt cgctacctgc tagttgcagg cgctgccgac tgcagtgccc
    10561 gagctagttg cagccgctaa ttgcaggcgc tgccgactgc agtgcccgag ccgttgagca
    10621 gcgtgcccgt gcatgtcatc tgaccggggc acatgccggt gcacgtcgct gccgtgcagc
    10681 cattcacgcc gtcaccggcg caggtcacgc cgcagaagtc catcggcgca ccgccgttga
    10741 acgtgatctg ctcgatgcag cccgcgacgc cagggcccgt gtagccctgc gcgcacgtat
    10801 cactcgcggt gttgcacggc ttgctgcacc acggatgcga cgcacccgtg aggttcaggc
    10861 agatgtagcc cgtcgggcag tcgccctgcc cgctgccttg cgcgggcggc gtgcacgcct
    10921 gcccgaggcc gaccgtgttg ttgctgcccg tgccaggcgc atccatgaac accttcgcgt
    10981 ccatgaacac tttcgcgtcc ggatcgccat gcgagtgcgt gccgccgtca tcgccaccac
    11041 acgcgaacat cgttgcaccg accgtgacgg cagcgaccac cgaaacagac agcaggcggc
    11101 tcgtcgacat gcgccgagcg tagcaccgta gtcgatacgt cgtcgtatgt gtccgaacgc
    11161 acgtgctcgc tcgcgtcgat gtttcatgtg ctcgtactcg tacacgtcca cgtgccacgt
    11221 gcacgtactc gtacacgtcc acgtgccacg tgctcgtaca cgtccacgtg ccacgtgcca
    11281 cgtgctcgtt cacggagccg cccccggagt gatttgcccc gttgatccgg agatcgaagg
    11341 acacagggcc gggcgcgaag agcgttgccg ccgagcacga gcacgagcac gagcacgagc
    11401 acgagcacga g
//
LOCUS       DATLBZ010000374         5902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9380372, whole genome shotgun sequence.
ACCESSION   DATLBZ010000374 DATLBZ010000000
VERSION     DATLBZ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9380372"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VMZ53_19170"
     CDS             <1..1080
                     /locus_tag="VMZ53_19170"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS30643.1"
                     /translation="TEGVQKDTGSAGASMKAPEEKPLTGADLAKKYQTCIGLINDNKL
                     DAFKKDCVDAGYKGHDNGMDMPPGTDALIEQFTGMRKAMPDYKLTPQLIVVSGRNILA
                     VELISGTHTGELAMGDGPAIPASNKKVGMLFFHRLAINDQNKAAEEWAFSDSTTMMAQ
                     LGLAPKDAPPKRPAMEKGWDNAPVVVVTADDAKEKANLDAFKKSMDAVNSHKTADLMA
                     LLTDDAVESDTTDAADRKGKKDIEAGMKMFFGAFSDAKVTADNTWAAGDYVIATGKFD
                     GTNDHDLGKIKKTGKKVSLDYAEVALIKDGKVAQLWRFHDGMQFAMQLGLMPPPGAAP
                     ATGSAAAPAGTTTPAPAGSAAPATK"
     gene            1122..2027
                     /locus_tag="VMZ53_19175"
     CDS             1122..2027
                     /locus_tag="VMZ53_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30644.1"
                     /translation="MRVRRIAGLAASGIVAVACVWLVGGAIAQVSLPTASAPQDAHLL
                     TLAAQAGRHWRIDGPRGPIHVWTPPGYRAETAATILYIHGYYDNADTAFVGHRLPEQF
                     AMSALNAMFIVVEAPHKQAVPVNYPSLSEVLRIVEDTTGVTRGMALTAAVGHSGAFRT
                     INDWLDEPLLEQLVMIDAMYANEELIEAWLKASPHHRLITVGEDTLQWNEQFARDTPE
                     TFIVDRVPPTYNTWPAQAKTARVVYVRAQYYHMPLVMDGVVLPSVLRLLPVELLATEP
                     WQQPLGAMPPYPDAALDAPTDSTTD"
     gene            2027..3544
                     /locus_tag="VMZ53_19180"
     CDS             2027..3544
                     /locus_tag="VMZ53_19180"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HUS30645.1"
                     /translation="MSPRQRIVAVIVVVLLGAYAYGAPGLRRQFMRAVIRWDAVREPA
                     VELPQGMGMDMSAAPRVRVVLIDGLSESTAQTLGTWSTLCKSGIDLDVDVGFPTVSLP
                     VQVSLWTGLTQQQTGVVFRSDRPFETPRADSIPGKVPGSIAIAENHGYIARSFAWGTV
                     LPPAEPGKPARDADADAWKTQWEQAALAATRSNSRLAFVHILRVDTWGHRKGRDSDEY
                     RTAAAEADAILGRLYAAAPDARWFLLADHGHLATGGHGGEEREVREVEHCIVGPGIEA
                     RKGGPVHLVDVSRAIADSAGVELDKHSQARPLSVALGAPLEDDVALPRLPLGRGVVAV
                     FIVAVGIALSLWSVRRWWLVPWWFVLAVIVLLLVRGQPTLSTPMTYTPEGRAMWTAWI
                     YVLPAATVAVWFALGSRRELGRRFGVVRIVTAQLALPLSTLFATLLACDGWFAMLGAH
                     DAPVVPHFTAYTSALLLLTAHGAAAVALAALARTVHSVIGRRPPPETPRSSPSAD"
     gene            complement(3429..5558)
                     /locus_tag="VMZ53_19185"
     CDS             complement(3429..5558)
                     /locus_tag="VMZ53_19185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUS30646.1"
                     /translation="MSLGRSHERTPGSWIVLAHMLGAAGIIGLDGFRLATGVGLAVLP
                     IAAATGLIAGLLVAGIERAVDGRPWWQVAIALAAPALIVYLPVSHSLFQGAYAQTLPL
                     ASAMPFLLPVVLWIVTALAIAIGRRVLRSGDLTTRGIIVLVLAGALGAVVWAERHVLG
                     SGYATAHIGATLAVAVLAGVAIRVTKRSGVPVPLTGMLGFIALGTAAAACVIGLRATE
                     HRALLATYGDQSRDLVALWRRIFDLDRDGTAAILGGGDCNDRDAKRHPGALDVPGDGI
                     DQDCDGVDPQVAAMSQEPAPQTKIDLDAWRASPDVAAILAKTKGMHIVVITVDALRAD
                     MLAADAPNRDDFPTLAKLLGDSVWFTRAFAPASGTDVSLATFLSGRHDPFQPLDSTLP
                     EALQKGGMTTFAALPNEVLRYAGETMLSRGITKVATIYTDWEQFNVGDHVSASATTKE
                     GLRAIADAAEKGKPAFIWLHYFDVHEHHQIKVPDSMLSTVHPGSLPKEHGYRALLRGI
                     DTEVGRVLGELDAKGIADKTIVVFASDHGESLGEDKRLLDTHGKVTYAPLVRIPIAIR
                     IPGVVPGQRTEQVSLVDLAPTLLALVGIPADAPFDGHDLVPTLLGAPQPLRNAGHPIA
                     IHEELQWSLVDWPHQLIVRPEDNLVELYDLEQDPKETTDLASKLPDVVNRLKASYAAF
                     PVVVVDRSPNGRSVREQRARQRPHHAQ"
     gene            5600..>5902
                     /locus_tag="VMZ53_19190"
     CDS             5600..>5902
                     /locus_tag="VMZ53_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30647.1"
                     /translation="MGVIRVVAALAFVAACDKGKVEPTTGSAQPPPAPLPVAIDAMSI
                     DEKILNGYPLKVEVRLCNDTGKTMDAVEWHQSFKATALKTGACTAYQETTGAYGYTY"
BASE COUNT         1030 a         1964 c         1956 g          952 t
ORIGIN      
        1 accgagggcg tccagaagga caccggctct gcaggcgcgt cgatgaaggc gcccgaggag
       61 aagccgctca ccggcgctga tctcgcgaag aaatatcaaa cgtgcatcgg gctcatcaac
      121 gacaacaagc tcgacgcctt caagaaggac tgcgtcgacg caggctacaa gggccatgac
      181 aacggcatgg acatgccgcc gggtacggac gccctgatcg agcagttcac cggtatgcgg
      241 aaggcgatgc cggactacaa gctgacgccg cagctgatcg tcgtcagcgg ccggaacatc
      301 ctcgcggtcg agctgatcag cggaacgcac accggcgagc ttgcgatggg cgatgggccc
      361 gcgatcccag cgagcaacaa gaaagtgggc atgctgttct tccaccgcct cgcgatcaat
      421 gatcagaaca aggccgcaga ggagtgggcg ttctccgaca gcacgacgat gatggcgcag
      481 ctcggtctcg cgcctaagga cgcgcccccg aagcgcccgg cgatggagaa gggctgggac
      541 aacgcgcccg tcgtcgtcgt caccgccgat gatgcgaagg agaaggcaaa cctcgacgcg
      601 ttcaagaagt cgatggacgc ggtcaactcg cacaagacgg ccgacctcat ggcgctgctc
      661 acggacgacg cagtcgagtc ggacacgaca gacgccgccg accgcaaggg caagaaggac
      721 atcgaggccg gcatgaagat gttcttcggt gcattctccg acgcgaaggt caccgcggac
      781 aacacgtggg ccgccggcga ctacgtcatc gcgacgggca agttcgacgg tacgaatgat
      841 cacgacctcg gcaagatcaa gaagacgggc aagaaggtct ccctcgacta cgccgaagtc
      901 gcattgatca aggacggcaa ggtcgcccag ctgtggcgat tccacgatgg gatgcagttc
      961 gcgatgcagc ttggcctgat gccgcctccg ggcgcggctc cggcgaccgg cagcgcagcc
     1021 gcaccggcgg gtaccaccac tcctgcgccc gctggcagcg cagcacccgc gacgaagtag
     1081 cgtaccgcgc gcgcggcgaa gcgccttgct atcatctgca cgtgcgtgtt cgccgcatcg
     1141 ctgggctcgc tgcgagcggc attgtcgccg tcgcttgcgt gtggctcgtc ggcggcgcga
     1201 tcgcgcaggt ctcgctgccg accgcatcgg ctccgcagga tgcgcatctg ttgacgctcg
     1261 ccgcacaagc gggacgccac tggcgcatcg atggcccgcg cggcccgatc cacgtgtgga
     1321 cgccaccggg ctatcgcgcc gagaccgcag cgacgatcct ctatatccac ggctactacg
     1381 acaacgccga taccgcgttc gtcgggcatc gcttgccgga gcagtttgcg atgtccgcgc
     1441 tcaacgcgat gttcatcgtc gtcgaagcgc cgcacaagca agcggtgccc gtgaactatc
     1501 cgagcctctc cgaggtactg cgcatcgtcg aggacacgac gggcgtcacg cgcggcatgg
     1561 ccctgaccgc ggcggtcgga cactccggtg cgtttcgcac gatcaatgat tggctcgacg
     1621 agccactgct cgagcagctc gtgatgatcg atgcgatgta cgcgaacgaa gagctcatcg
     1681 aggcgtggct caaggcgtcg ccgcatcacc ggctgatcac ggtcggcgaa gacacgctgc
     1741 agtggaacga gcagttcgcg cgcgacacgc ccgagacgtt catcgtcgat cgcgtaccgc
     1801 cgacgtacaa cacgtggccc gcgcaagcga agaccgcgcg agtcgtgtac gtgcgcgcgc
     1861 agtactatca catgccgctc gtcatggacg gtgtcgtgct gccgtcggtg ctgcggctct
     1921 tgcccgtcga gcttctcgcg accgagccgt ggcaacagcc gctcggcgcg atgccgccct
     1981 accccgacgc cgcgctcgac gctcccacag acagcacgac ggactgatgt cgccgcgcca
     2041 gcgcatcgtc gcggttatcg tcgtcgtgtt gctcggtgcg tacgcgtatg gtgcgcccgg
     2101 attacggcgt cagttcatgc gcgcggtgat ccggtgggac gccgttcgcg agccagccgt
     2161 cgagcttccg cagggcatgg ggatggacat gtcggccgcg ccgcgcgtgc gcgtcgtgct
     2221 gatcgacggg ctctccgagt cgaccgcgca aacactcggc acgtggtcga cgctctgcaa
     2281 gagcgggatc gatctcgacg tcgatgttgg ctttccgacc gtgtcgttgc ccgtgcaggt
     2341 ctcgctgtgg acgggtctca cgcagcagca gacgggcgtt gtatttcgat ccgatcggcc
     2401 attcgagacg ccgcgcgccg acagcattcc cgggaaagtc cccggcagca tcgcgatcgc
     2461 cgagaaccac ggctatatcg cgcgcagctt cgcgtggggc actgtccttc cgcccgcaga
     2521 gccgggcaag cccgcgcgcg atgccgatgc cgacgcgtgg aagacgcagt gggaacaagc
     2581 cgcactcgcg gcaacgcgca gcaattcgcg gctggcgttc gtccacatcc tccgcgtcga
     2641 tacgtgggga catcgcaagg gccgcgactc cgacgagtat cgaaccgccg ccgctgaagc
     2701 ggacgcgatc ctcggcaggc tctacgccgc agcacccgac gcgcgctggt tcttgctcgc
     2761 cgatcacgga catctcgcca ccggtggtca cggcggcgaa gagcgcgagg ttcgcgaggt
     2821 cgagcactgc atcgtcggtc ccgggatcga ggcgcgcaaa ggcgggccgg ttcatctcgt
     2881 cgacgtgtcg cgcgccatcg ccgattcggc aggtgtcgag ctcgacaagc attcgcaggc
     2941 acggccgctg tcggtcgcgc tcggagcgcc gctcgaggac gatgtggcac tgccgcggtt
     3001 gccactcggg cgcggcgtgg tcgcggtgtt catcgtcgcg gtcgggatcg cgctctcgct
     3061 gtggagcgtc cggcgctggt ggctcgtgcc gtggtggttc gtgctcgcgg tcatcgtgct
     3121 gctgctcgtg cgcggccaac cgacgctctc gacaccgatg acgtacacgc ccgaagggcg
     3181 cgcgatgtgg accgcgtgga tctatgtgtt gcccgcggca accgtcgccg tgtggttcgc
     3241 gctcggcagc cggcgcgagc tcggtcgcag attcggggtt gttcgcattg tcacggcgca
     3301 gctcgccctg cccctctcga cgctgttcgc gacgctactc gcgtgtgacg gctggttcgc
     3361 gatgctcggt gctcacgacg cgccggtcgt cccgcacttc acggcgtata cgtcggcgct
     3421 gctcctgctc actgcgcatg gtgcggccgc tgtcgcgctc gctgctctcg cacggaccgt
     3481 ccattcggtg atcggtcgac gaccaccacc ggaaacgccg cgtagctcgc cttcagccga
     3541 ttgacgacat ccggcagctt cgacgcgaga tccgtcgtct ccttcggatc ctgctcgaga
     3601 tcgtagagct cgacgaggtt gtcttccggt cggacaatca gctgatgcgg ccaatcgacg
     3661 agcgaccact gcagctcctc gtggatcgcg atcggatggc cggcattgcg cagcggctgg
     3721 ggggcgccaa gcagcgtcgg gacaagatca tgaccgtcga acggcgcatc ggcggggatt
     3781 ccaacgagcg cgagcagcgt cggcgcgaga tcgacgagcg acacttgctc ggtgcgctgg
     3841 cccggcacga ctccaggaat gcgaatcgcg atcggaatgc ggacgagcgg tgcgtaggtg
     3901 actttgccat gcgtatcgag aagtcgtttg tcttcgccga gcgactcacc gtgatcgctt
     3961 gcgaacacga cgatcgtctt gtcggcaatg ccctttgcgt cgagctctcc gagcacgcgg
     4021 ccgacttccg tatcgatgcc gcgaagcagc gcgcgatagc cgtgctcttt cggcaggctt
     4081 ccgggatgca ctgtcgacag catcgagtcg ggcaccttga tctggtgatg ctcgtgaacg
     4141 tcgaagtagt gcagccagat gaatgcgggc ttgcccttct ccgccgcgtc ggcgatcgcg
     4201 cgcaggccct ctttcgtcgt cgcgctcgcg ctcacgtggt cgccgacgtt gaactgctcc
     4261 cagtcggtat agatcgtcgc gaccttcgta atgccgcggc tgagcatcgt ctcgccggcg
     4321 taccgcagca cctcattcgg caaggctgca aacgtcgtca tgccgccctt ctgcagcgcc
     4381 tcgggcagcg tcgaatcgag cggctgaaac ggatcgtgcc gtcccgacag gaacgtcgcg
     4441 agcgagacat ccgttccgct ggccggcgca aacgcgcgcg tgaaccacac cgaatctcca
     4501 agcagctttg cgagcgtcgg aaaatcatcg cgattcggcg cgtcggcggc gagcatgtct
     4561 gcgcgaagcg cgtcgacggt gatgacgacg atgtgcatgc ccttcgtctt ggcaaggata
     4621 gcggcgacgt cgggtgatgc tctccacgca tcgaggtcga tcttcgtctg tggcgcgggc
     4681 tcctggctca tcgccgcgac ttgcggatcc acgccgtcgc agtcttgatc gatcccatcg
     4741 ccgggaacat cgagcgcacc gggatgccgc ttcgcgtcgc gatcgttgca gtcgccaccg
     4801 ccgaggatgg ccgcggtgcc atcgcgatcc agatcgaaga tgcgccgcca cagtgcgacg
     4861 aggtcgcggc tctgatcgcc gtacgtcgcg agcagagcgc gatgctctgt cgctcgcagg
     4921 ccgatcacgc acgcagccgc tgccgtgccg agcgcgatga atccgagcat gcccgtcagc
     4981 ggcaccggca caccactccg cttcgtcacg cgaatggcca cgccggcgag cacagcgact
     5041 gcgagcgtcg cgccgatatg cgcggtcgcg tacccgctgc cgaggacgtg acgctcggcc
     5101 cacacgactg cgccgagcgc gccagcaagc acgagcacga tgatgccgcg cgtcgtcaga
     5161 tcgcccgagc gcaagacgcg gcggccgatc gcaattgcga gcgcggtcac gatccacagc
     5221 acgaccggca agagaaacgg catcgcgctc gcgagcggca gcgtctgcgc gtacgcgccc
     5281 tgaaacagcg agtgcgagac cggcaagtag acgatcaacg ccggtgcagc gagcgcgatc
     5341 gcgacctgcc accacggccg gccatcgacg gcgcgctcga tccccgcgac gagaagtccc
     5401 gcgatcagcc ccgtggctgc ggcaatcggc aggaccgcga ggccaactcc cgtggcgagg
     5461 cgaaatccgt cgaggccgat gatgccggcc gcgccgagca tgtgcgccag gacgatccac
     5521 gagcccggcg tgcgctcgtg actgcggccc agggacatcg agcgaatgct agcgcacggg
     5581 cgctgctatg ctgagggaga tgggcgtgat tcgagtcgtg gcagctctcg cgttcgtcgc
     5641 tgcgtgcgac aagggcaagg tcgagccgac gacgggctct gcgcagccgc cacccgcgcc
     5701 gctgcctgtc gccatcgacg cgatgtcgat cgacgagaag attctcaacg gctacccgct
     5761 gaaagtcgaa gttcgcctgt gcaacgacac gggcaagacg atggatgccg tcgagtggca
     5821 tcagagcttc aaggccaccg ccctgaagac tggtgcgtgc accgcctatc aagaaacgac
     5881 cggcgcgtac ggctacacct ac
//
LOCUS       DATLBZ010000375         6099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1188489, whole genome shotgun sequence.
ACCESSION   DATLBZ010000375 DATLBZ010000000
VERSION     DATLBZ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188489"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VMZ53_19195"
     CDS             <1..180
                     /locus_tag="VMZ53_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30648.1"
                     /translation="YDSLEVWTWKNNAWAKVKTLTGPVGTITTEEFLGRYHYLRFVSD
                     SSVTGQGVTLDAEWR"
     gene            214..795
                     /locus_tag="VMZ53_19200"
     CDS             214..795
                     /locus_tag="VMZ53_19200"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HUS30649.1"
                     /translation="MKKRALFIFGALIGCSGSEPATTDAAVDTPPDMFIYDEPGIPMT
                     LTSPTITEGMPIPLTHVCANRGGQNQSPQLTINNVPSGTMSFAVVLTDLSNQLVHCAI
                     YDVPSSTTTLPANIDKNPSPTAVPGAKQTPSYNTNVIGYNGPCPGSLHTYQFKVYALG
                     AATLSGITTKESVVTAASASNLGTATLTGTFTP"
     gene            860..1255
                     /locus_tag="VMZ53_19205"
     CDS             860..1255
                     /locus_tag="VMZ53_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30650.1"
                     /translation="MSFRNDLDAALARIDVLERENCELIAENTKLRGAESPHAPRTMR
                     PSHRSDPEAASAHVALLERTNLQLADENRRLAVGAPPAIAEPAPRPWPYDDLRDLRIE
                     KLGVSWNFHPVVGILILALALLMAWAFPR"
     gene            complement(1252..2175)
                     /locus_tag="VMZ53_19210"
     CDS             complement(1252..2175)
                     /locus_tag="VMZ53_19210"
                     /inference="COORDINATES: protein motif:HMM:NF016932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HUS30651.1"
                     /translation="MSQAAADSLVEQGNELFRAEQFADAAAHFERAVNVFPHHALGWK
                     GLGHALLCMGKPHEAARAFDQAIGLSPDSATALWGGALAHAEIGNKVIARDYLRRTIA
                     LQPTWMSMVANAPQLASLLQVSARTADRLRELFGAFSTRRFTHAADEARAIEVGRIAN
                     QPEFGKYTYVTLGLTNHEWPETERPRIELLLVSTVDTDACAQIVANLAFHLADTNFFP
                     EPGTMVRDVVGALGAGDLSERLPHVYVQSPRSWLNLELPLDEGPPAVTLAQVFPISEE
                     EYQAWKGLGAQRFEQSIVDRKVDIADLRRAG"
     gene            2225..2746
                     /locus_tag="VMZ53_19215"
     CDS             2225..2746
                     /locus_tag="VMZ53_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30652.1"
                     /translation="MTAAVGADVEALCSKCGDVWHVVVAKVGAQIVKVQCKQCGGYHR
                     YKSPHGAPKEKRQPSLYGEKPARAAAASAAPKAPVERFEKPAVAADLSKPSRTYSAVL
                     RYEVGERVDHVQFGQGVVETAEPGKITVFFASGRRVLVQSKEGAPGASSKLERPKPFD
                     HSNAAGGKRVGET"
     gene            2747..3385
                     /locus_tag="VMZ53_19220"
     CDS             2747..3385
                     /locus_tag="VMZ53_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30653.1"
                     /translation="MAKKKWLGYVGPAIVILGIAVSVVAVWYMRAARPVPGAEFDRIP
                     IDDSHAFVIRLDAKDPLRSFLELRERDRVMWQALIPHYIGSPGRPAIAWSPNIVTVRV
                     ERDGRAEVFAFAMTTAVKVGALRLAPEHEPITTHAQGPITLTDHVRSYELAGGAGWNQ
                     LVAVGLDTGKGVWKVDLGAAPITDGGVDGSTLWLVQGGKRLTFDAATGRATE"
     gene            3454..5721
                     /gene="ppk1"
                     /locus_tag="VMZ53_19225"
     CDS             3454..5721
                     /gene="ppk1"
                     /locus_tag="VMZ53_19225"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HUS30654.1"
                     /translation="MAAERPIVAPEPTSSGVHRLELLVEPEQRPAAKPIDIPPGRTPP
                     APGTGDIVPTDLGAPELYLNRELTYLNFCWRVLHEAEDDRLPLLERVKFLAIVSSNLD
                     EFFMKRIGGLKQQVGAQLHSITPDGRTPAQQILDCIDLITKLERRKAGILEKVKAELK
                     AVGVWIAPYKELDPQQAAWVREYYVKNIFPLVTPQAMDPAHPFPFVSNLSLNLLVSLR
                     YEHDSESLLARVKVPVGGGMSRFVRLGTSRTFVDLTEVMANNLDLLFPGMEIEACSLF
                     RVTRNAITERDEEEADDLLELIEIEMRKRKFAPVVRLQVEREMQPHLRGRIAAELGLD
                     EEQDVFEAVGMFGQRDLFEIATLDIPELRDTAHAPAQPVDFITDGNIFHAIREAKHLL
                     VHHPYESFQASVERFFREAADDPKVRAIKTTLYRTSKDSGVIKHLVRAAQNGKQVAVV
                     LELKARFDEEANIKWASRMERSGIHVTYGVVGLKTHTKSTLVVRHDYDGLRRYAHVAT
                     GNYHSGTARLYTDLGLFTCDDDIGRDLTELFNYLTTGYKPRRKYKKLLVAPKQLKPAL
                     LEKIEREIALVGQGERGHIQWKLNALEDVDIVRALYRASQRGVTIDLIVRDTCRLRPG
                     LDGVSSSIKVVSTIGRFLEHARIYYFHNGGRAEYYIGSADTMQRNLEKRVEVLVPIED
                     ARLQQELRFILDTQLSDQRGTWDMLPDGSYSQRTGLNAKHSQQQMIERTERRLKEATR
                     LRRRKVQTTTSRKKR"
     gene            complement(5718..>6099)
                     /locus_tag="VMZ53_19230"
     CDS             complement(5718..>6099)
                     /locus_tag="VMZ53_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30655.1"
                     /translation="QRISTVTRGPKRHMFVIETNQQLHEWATDDNGTWSSVRTSTATN
                     LGVNLVFGMDMSADGRRMLMFARNIQSVDVMTYSYRDSIDVPFRPAVTLPDSPGSRDA
                     FLSEDCDRMYFSGLDAVFYVRRLP"
BASE COUNT         1130 a         1980 c         1968 g         1021 t
ORIGIN      
        1 tacgactcgc tcgaagtctg gacctggaag aacaacgcgt gggccaaggt caagacgctc
       61 accggcccgg tcggcacgat cacgaccgag gagttcctcg gccgctatca ctacctgcgc
      121 ttcgtgtccg attcgtcggt caccggccag ggcgtgacgc tcgacgccga atggcgctaa
      181 cgctcgactc caatcagcgt tagcctcggg cccgtgaaga agcgggccct tttcattttc
      241 ggcgctctca tcggatgcag tggcagcgag ccggcgacga ccgatgcagc ggtcgacacg
      301 ccgccggaca tgttcatcta cgacgagccc ggcatcccga tgacgctgac gagcccgacg
      361 atcacggaag gtatgcccat cccgctgacg cacgtttgcg cgaaccgcgg cggccagaat
      421 caatcgccgc agctgacgat caacaatgtc ccgagcggca cgatgagctt cgcggtggtg
      481 ctcaccgatc tgtcgaacca gctcgtccac tgcgcgatct acgacgtgcc gagcagcacg
      541 acgacgcttc cggcgaacat cgacaagaac ccgtcgccga ccgcggtgcc cggtgcgaag
      601 caaacgccga gctacaacac gaacgtcatc ggctacaacg gcccgtgccc cggcagcctc
      661 cacacgtatc agttcaaggt gtacgcgctc ggtgccgcga cgctctccgg catcacgacg
      721 aaagagtcgg tcgtgacagc tgccagcgcg tcgaacctcg gcaccgcgac gctcacggga
      781 acgttcacgc cctagcgcta accctgcggc gtgtcgtgtt tcgcgtgaca gcgcgccgag
      841 cgatgcgcag ctttttcgca tgagctttcg caacgacctc gatgccgcac ttgctcggat
      901 cgatgtgctc gagcgcgaga actgcgagct cattgcagag aacacgaagt tgcgcggtgc
      961 ggagtcgccg catgcgccgc gaacaatgcg tccgagtcat cgcagcgatc ccgaggccgc
     1021 ctcggctcac gttgcgctgc tggaacgtac gaatctgcag cttgccgacg agaacagacg
     1081 attggcggtc ggcgctcccc ctgcgattgc agagcctgcc ccgcggccat ggccgtacga
     1141 cgatctgcgc gacctccgga tcgaaaagct cggagtgagt tggaatttcc accctgtcgt
     1201 agggatcctc atactcgcgc ttgcgctact catggcctgg gcgtttcctc gctaacccgc
     1261 gcggcgaagg tcggcgatgt cgaccttgcg atcgacgatc gactgctcga agcgctgcgc
     1321 gccgagtccc ttccacgctt ggtactcctc ttcgctgatc gggaagacct gcgcgagtgt
     1381 gacggcgggc gggccttcgt cgaggggaag ctcgaggttc agccacgagc gcggcgattg
     1441 gacgtagacg tgaggcaggc gctcggagag atcgcccgcg ccgagcgcac cgacgacatc
     1501 gcgcaccatc gttcccggct ctgggaagaa gttggtgtcg gcgagatgga aggcgaggtt
     1561 cgcgacgatc tgtgcgcagg cgtccgtgtc gacggtggag acgaggagca gctcgatgcg
     1621 cggccgttcg gtctccggcc actcgtgatt cgtgaggccg agcgtcacgt aggtgtactt
     1681 gccgaattcg ggttggttcg caatgcgccc gacttcgatc gcgcgtgctt cgtcggcggc
     1741 gtgggtgaag cggcgtgtgg agaaagcgcc gaacagctcg cgcaggcgat cggcggtgcg
     1801 ggcgctgact tggaggagcg acgcgagctg cggggcattc gcaaccatcg acatccacgt
     1861 cggttgcaat gcgatcgttc gtcgcaggta gtcgcgcgcg atcaccttgt tgccgatttc
     1921 ggcgtgggcg agcgcaccac cccagagggc ggtggcggag tcgggcgaga ggccgatcgc
     1981 ttgatcgaac gcgcgtgcgg cttcgtgcgg cttgcccatg caaagcagag cgtggccgag
     2041 acctttccag ccgagggcgt ggtgcggaaa cacgttcact gcgcgctcga agtgggccgc
     2101 cgcgtctgcg aattgctcgg cgcggaacag ttcgtttcct tgttcgacaa gcgagtctgc
     2161 ggcggcctga ctcatgggcc ccgggcgtag cacgattgct ttagcttcct gctataactc
     2221 gcgcatgact gccgctgttg gagcagacgt cgaagccctt tgctccaagt gtggtgacgt
     2281 ctggcacgtc gtggtcgcga aagtcggcgc gcaaatcgtc aaagtgcagt gcaagcagtg
     2341 cggcggctat caccgttaca agtcgccgca cggggccccg aaagagaaga gacagccctc
     2401 actgtatgga gagaagccgg cacgggcggc agcagcatcg gccgcgccga aagctcccgt
     2461 cgagcgcttc gagaagccgg ccgtcgccgc ggatctgagc aagccgtcgc gcacgtactc
     2521 cgcggtgctg cgctacgagg ttggcgagcg cgtcgatcac gtgcagttcg gacaaggcgt
     2581 tgtcgagacg gcggagcccg gaaagatcac ggtctttttt gcgtcggggc gccgcgtgct
     2641 cgtgcaatcg aaagagggcg cgccgggagc atcgagcaag ctcgagaggc ccaagccgtt
     2701 tgatcattcg aacgcggcgg gcggtaagcg cgtcggcgag acctgagtgg cgaagaagaa
     2761 gtggctcggc tacgtcgggc ccgcgatcgt cattctcggg atcgccgtgt cggtcgtcgc
     2821 cgtttggtac atgcgcgcag ccaggcccgt gccgggcgcg gaattcgatc gcatcccgat
     2881 cgatgactcg cacgcgttcg tgatccggct cgatgcgaag gatccgctgc gctcgtttct
     2941 agagctccgc gagcgcgacc gcgtgatgtg gcaggcgctg attccgcatt acatcggatc
     3001 gcccggacgt cccgcgatcg cgtggagccc gaacatcgtc accgtgcgtg tggagcgcga
     3061 cggtcgtgcg gaagtgttcg cgttcgcaat gacgacagcg gtgaaggtcg gcgcactccg
     3121 gttggcgccg gagcacgagc cgatcacgac gcacgcgcag ggaccgatca cgctgaccga
     3181 tcacgtgcgc agctacgagc tcgcgggcgg cgccggttgg aaccagctcg tcgcggtcgg
     3241 cctcgatacc ggcaaaggcg tctggaaggt cgacctcggg gccgctccga ttacggatgg
     3301 cggcgtagac gggagtacgc tctggctcgt gcagggcggg aagcgcctca cgttcgacgc
     3361 cgcgaccggt cgtgcaactg aataaaatcg caggacaata ctgacagagc cgttgtaacg
     3421 gtcccgtcac cggggcgcta tactttgctc ttcatggctg ctgaacgtcc catcgtggcg
     3481 cccgagccga ccagctcagg cgttcaccgg ctcgagctgc tcgtcgagcc cgagcaacgc
     3541 cccgcggcca aaccgatcga catcccgccg ggccggacgc cgcctgctcc cggaaccggc
     3601 gacatcgtgc cgaccgatct cggcgcgccc gagctctatc tcaatcgcga gctgacgtac
     3661 ctcaacttct gctggcgcgt cctgcacgaa gccgaagacg atcgcctgcc gctgctcgag
     3721 cgcgtgaagt ttctcgcgat cgtgagctcg aatctcgacg aattcttcat gaagcgcatc
     3781 ggcggcctca agcagcaggt cggcgcgcag ctgcattcga tcacgccgga tggccgcaca
     3841 cccgcgcagc agatcctcga ttgcatcgat ctcatcacga agctcgagcg ccgcaaggcg
     3901 ggaatcctcg agaaggtgaa agcggagctc aaggctgtcg gcgtgtggat cgctccctac
     3961 aaggagctcg atccgcaaca ggccgcgtgg gtgcgcgagt actacgtcaa gaacatcttc
     4021 ccgctcgtca cgccgcaggc gatggatccg gcgcacccgt ttccattcgt gtcgaacctg
     4081 tcgctgaact tgctcgtgtc actgcgttac gagcacgaca gcgagtcgct gctcgcacgc
     4141 gtgaaggtgc cggtcggcgg cggcatgtcg cgcttcgtgc ggctcggcac gtcgcggacg
     4201 ttcgtcgatc tcaccgaggt gatggcgaac aacctcgatc tgctgttccc cggcatggag
     4261 atcgaggcgt gctcgctgtt ccgagtgacg cgcaacgcga tcaccgagcg cgacgaagaa
     4321 gaagccgacg atctcctcga gctgatcgag atcgagatgc gcaagcgcaa gttcgcgccg
     4381 gtcgtccgtc tgcaagtcga gcgcgagatg caaccgcacc ttcgcggccg catcgccgcc
     4441 gagctcggtc tcgacgagga gcaggacgtg ttcgaggcgg tcggcatgtt cggccagcgc
     4501 gacctattcg agatcgcgac gctcgacatt cccgagctac gcgataccgc gcacgcgccc
     4561 gcgcagcctg tcgacttcat caccgacggc aacatcttcc acgcgatccg cgaagcgaag
     4621 catctgctcg ttcatcatcc gtacgagtca ttccaggcat cggtcgagcg tttcttccgc
     4681 gaagccgccg acgatccgaa ggtccgcgcg atcaagacga cgctctaccg cacgtcaaaa
     4741 gactcgggcg tcatcaagca tctcgtgcgc gccgcgcaga acggcaagca ggtcgcggtc
     4801 gtcctcgagc tcaaagcacg gttcgacgaa gaagcgaaca tcaagtgggc gtcgcgtatg
     4861 gagcgctcgg gcatccacgt cacgtacggc gtcgtcgggc tcaagacgca cacgaagtcg
     4921 acgctcgtcg tccgccacga ctacgacggc ctgcgtcgct atgcgcacgt cgcgacgggc
     4981 aactaccact cgggcaccgc gcgcctctac acggacctcg gcctcttcac ctgcgacgac
     5041 gacatcggtc gcgatctcac cgagctgttc aactacctga cgacgggcta caagccgcgc
     5101 cgcaagtaca agaagctgct cgtcgcgccg aagcagctca agcccgcgct gctcgagaag
     5161 atcgagcgcg agatcgcact cgtcggccaa ggcgagcgcg gccacatcca gtggaagctc
     5221 aacgcgctcg aggatgtcga tatcgtgcgc gctctctatc gcgcgtcgca gcgcggcgtg
     5281 acgatcgatc tgatcgtccg cgacacatgc cggctgcggc caggcctcga cggcgtgtcc
     5341 agctcgatca aagtcgtctc gacgatcggc cgcttcctcg agcacgcccg catctactac
     5401 ttccacaacg gtggacgcgc cgagtactac atcggctcgg cggacacgat gcagcgcaac
     5461 ctcgagaagc gcgtcgaagt gctcgttccg atcgaggacg cgcgcctgca gcaggagctg
     5521 cgcttcatcc tcgatacgca gctgtccgat cagcgcggca cgtgggacat gctgccggat
     5581 ggcagctaca gccagcgcac cggcctcaac gcgaagcaca gccagcagca gatgatcgag
     5641 cgcaccgagc gccggctcaa agaggccacg cgcctgcgcc gccgcaaagt ccagacgacg
     5701 acttcgcgga aaaagcgtta aggcaggcgc cgcacgtaga agaccgcgtc gaggccggag
     5761 aagtacatcc ggtcgcagtc ttcgctgaga aacgcgtcgc gactgcctgg cgagtcgggc
     5821 agcgtgaccg cgggccgaaa cgggacgtcg atcgaatcgc gatacgaata cgtcatcacg
     5881 tccacagact ggatattgcg cgcgaacatc aacatccgcc ggccgtccgc ggacatgtcc
     5941 ataccgaaca ccagattcac gccgagattc gtcgcggtgg acgtgcgcac ggagctccac
     6001 gtgccgttgt cgtcggtcgc ccactcgtgc agctgttgat tcgtctcgat gacgaacatg
     6061 tggcgcttcg gcccgcgcgt caccgtcgag atccgctgc
//
LOCUS       DATLBZ010000376         5209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9381704, whole genome shotgun sequence.
ACCESSION   DATLBZ010000376 DATLBZ010000000
VERSION     DATLBZ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9381704"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VMZ53_19235"
     CDS             complement(<1..792)
                     /locus_tag="VMZ53_19235"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30656.1"
                     /translation="MSGQAAEQIIAGVVLSERLAVTMYGAIHRAQFGGQRNLRGLVVD
                     PKMLEEDAFRISLTDAKNIAAVTKLEHASIVPTVTVESGGPDVVIVSRGGGRYVTVQD
                     LIAGAKARSKQGGKLSIPIAGLIGKSVIEALAAAHKAGLIHGAVHPRSVLIDEDGGVR
                     LTDFIAGRALTTAVAQGADSSLWRGIAGYIAPELVVGEDPTPGVDVFAVGAMLFTMLS
                     GEAPPGSLRVTPAVERLVQRALDTDSSRRYKTANDMLENLLEAMED"
     gene            complement(802..1647)
                     /locus_tag="VMZ53_19240"
     CDS             complement(802..1647)
                     /locus_tag="VMZ53_19240"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HUS30657.1"
                     /translation="MVRDMEPLLTSTFGGALAVLLGLLWGSFANVCIYRWPPTDEHPK
                     GRSVVKPGSHCGSCKAPIAWYDNVPILSYLWLRGHCRNCKASFSPRYLLVEAVTAALF
                     GVAWWFTLGEGGFYYMTFEHALVKFLIDAAFIFVLVVIAFIDLDHMLILDKVTFPSIA
                     IAYIASLYDHHWWEGLIGAAIGYGLPWLIGWVYLKLRDREGLGLGDGKLLALVGALLG
                     WKGVIVSLFGGALIGSIIGIVALTRAKSKDVMTSELPFGPFLSAAAVFYLFAEPWIVV
                     YFRLL"
     gene            1702..2685
                     /locus_tag="VMZ53_19245"
     CDS             1702..2685
                     /locus_tag="VMZ53_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30658.1"
                     /translation="MLSACPLARLAVATVALAGCGRSEGVPDKDLGSLVIAPKEKRDP
                     IDVTKATRDPAELGRAVMLPFKDVVAALGPNRWTIKTTNIVDEAGKTVSNLAETTKLS
                     LGAPPAFTALYENSADYGREAIFVGGKLYLRPRYQRWHGRAPETPDEPGELRDGFFGP
                     IGATWDLLAPAIELTDQGAATVAGRPGKKIALKQSPSPRTPAVEKLVQRKWREKRTIE
                     GVSGEVILDAEKGVPLSVKLAGTVGFMRDGRRFAMKVTLDAVLDGVGPVDVQAPPADQ
                     VVATPERLKEVDDRDFLLQGIAPPLRKNPDGTAVPPQPTPAGSASGSNSGK"
     gene            2682..3485
                     /locus_tag="VMZ53_19250"
     CDS             2682..3485
                     /locus_tag="VMZ53_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30659.1"
                     /translation="MTAGTALVQRRDYLAGQRRTIIARSLIGSLAGALPLPFLDDWAV
                     ARILGGGYERIAAAHHVDLHPDAVNALVHGATKPPTIANIAIGGVLARVASLAARRMM
                     VAIAAINRARSAARTYTAMTLFDHYCAKLHTGLELDGPTALLLREEIIRSIDQTPGAL
                     AFHPFRRAALSAARATVRAPLELVDLVSGGRLRKMLAAKSESSGVAEPEHIDDIELAM
                     EAALADKNNFLARTVASVEAQLSSEVNPFLDAAIESLDRRWRARVAAKR"
     gene            3482..4606
                     /gene="proB"
                     /locus_tag="VMZ53_19255"
     CDS             3482..4606
                     /gene="proB"
                     /locus_tag="VMZ53_19255"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HUS30660.1"
                     /translation="MTQSPDVRHERAALKDAKRVVVKIGSRLIADAPASRPATIADQV
                     AELRRRGVEVVIVSSGAIALGLPRLKMKQRPHEMPLLQAAAAVGQSRLMQQWEHAFGA
                     HQIEIGQVLVTHDDLGDRRRFLSARLTLRALLDHGVVPIINENDTVASEEIKFGDNDQ
                     LAALVCNLVSADLLIILTDVEGVRDAHGIRMPIVHDIDREAVPVAGGSTSGVGSGGMA
                     SKVGSARIVTRTGIPAVVAPGREAEVLTWVLAGVDVGTLFVPPARGAGVLSARKHWIA
                     YGAKPCGKLTVDDGAVKALTTGGKSLLPAGIIAVEGDFELGDTVSIVTQNGNEIARGL
                     AAYPGDDLRKIRGLQSAGIEATLGYKSLDEAIHRDDLVIL"
     gene            4603..>5209
                     /locus_tag="VMZ53_19260"
     CDS             4603..>5209
                     /locus_tag="VMZ53_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUS30661.1"
                     /translation="MTQLEREQLIRVSAQAAKAASRVVAKAPAAQRQKALLAIADAID
                     AQAPQILAANAKDVATASGLSAAMVDRLTLDAKRVAGLSRAVREVAAQEEILGRIDKT
                     DARPNGLEISRVRIPLGVIAMIYEARPNVTTDAAALCLKAGNACILRGGREALESNRA
                     LLRAVQTGLATAGLPAEAVQLIPVMEREAIADLVKLDELIDL"
BASE COUNT          926 a         1754 c         1723 g          806 t
ORIGIN      
        1 gtcttccatc gcttcgagca agttctcgag catgtcgttc gcggtcttgt agcgacgcga
       61 cgaatcggta tcgagcgcgc gctggacaag ccgctcgacg gcaggcgtga cgcgcagcga
      121 tccaggcggc gcttcgcccg agagcatcgt gaacagcatc gcgccgactg cgaatacgtc
      181 gacgccgggc gtcgggtcct cgccgacgac gagctccggc gcgatgtacc cggcgatgcc
      241 gcgccacagc gaagaatcgg cgccttgcgc gaccgccgtc gtgagcgcgc ggccggcgat
      301 gaaatcggtc agccgcacgc cgccgtcctc gtcgatcagc acgctgcgcg ggtgcacagc
      361 gccatggatg agacccgctt tgtgcgcggc cgcgagcgcc tcgatgacgc tcttgccgat
      421 gaggccagcg atcggaatcg acagcttgcc gccctgcttg ctccgcgcct tcgcgcccgc
      481 gatgagatcc tgcaccgtga cgtagcgacc gccgccgcgg ctgacgatca cgacgtccgg
      541 gccacccgat tcgacggtca cggtcggcac gatgctcgcg tgctcgagct tggtgacggc
      601 ggcgatattc ttcgcatccg tgagcgagat gcggaacgcg tcttcttcca gcatcttcgg
      661 gtcgaccacg aggccgcgaa ggttgcgttg gccgccgaat tgagcgcgat ggatcgcgcc
      721 atacatcgtc actgcaaggc gctcggaaag cacgaccccc gcaatgatct gctcggccgc
      781 ttggcctgac atcgtcggta actatagcag ccggaagtac acgacgatcc acggctcggc
      841 gaacaaatag aacaccgccg cagccgagag aaacggccca aacggcagct cgctggtcat
      901 cacgtccttc gacttcgctc ttgtcagggc gacgattcca atgatcgagc caatgagcgc
      961 cccgccgaac agcgacacga tcacgccctt ccagccaagc agcgcaccga cgagcgcgag
     1021 cagcttgcca tcgccaaggc ccaggccctc gcggtcgcgc agcttcagat agacccagcc
     1081 gatgagccac ggcaggccat agccgatcgc cgcaccgatg aggccctccc accagtggtg
     1141 atcgtagagc gatgcgatgt aggcaatcgc gatcgacggg aacgtcacct tgtcgaggat
     1201 cagcatgtga tcgagatcga tgaacgcgat cacgacgagc acgaagatga acgcggcgtc
     1261 gatgaggaac ttcacgagcg cgtgctcgaa cgtcatgtaa tagaacccgc cctcgccgag
     1321 cgtgaaccac cacgcgacgc cgaacagcgc cgccgtcacc gcctcgacga gcaggtagcg
     1381 cggcgagaag ctcgccttgc agttgcggca gtggccgcgc agccacaggt agctgaggat
     1441 cggcacgttg tcgtaccagg cgatcggggc cttgcagctg ccgcagtgcg agccgggctt
     1501 cacgacggag cggcctttgg ggtgctcgtc cgtcggtggc cagcggtaga tgcagacgtt
     1561 cgcgaagctg ccccagagca ggcccaacag gaccgcgagc gcaccaccga aggtggaggt
     1621 cagcagcggc tccatgtctc gaaccacgcg aaagcctagc ttggttgacc ctggcggtcg
     1681 agaaagctaa gacctcggcg gatgttgtct gcctgccctc ttgcccgcct cgccgttgcc
     1741 accgtggcac tcgcgggctg cggccgctcg gagggcgtcc ccgacaaaga tctcggctct
     1801 ctcgtgatcg cgcccaaaga gaagcgcgac ccgatcgatg tcacgaaggc aacccgcgat
     1861 ccggccgagc tcgggcgcgc ggtgatgctg ccgttcaagg atgtcgtcgc cgcgctcggt
     1921 ccgaatcgct ggacgatcaa gacgacgaac atcgtcgacg aagcgggcaa gacggtgagc
     1981 aacctggcgg agacgacgaa gctctcgctc ggcgcaccgc cggcgttcac cgcgctctac
     2041 gagaacagcg ccgactacgg acgcgaagca atcttcgtcg gtggcaagct gtacctgcgc
     2101 cctcgctacc agcgctggca cggtcgcgcc ccggagacgc ccgacgagcc gggggagctg
     2161 cgcgacggct tcttcggtcc gatcggcgcg acgtgggatc tgctcgcgcc tgcgatcgag
     2221 ctgactgatc aaggcgcagc caccgtcgcg ggacggcccg gaaaaaagat cgcgctgaag
     2281 caatcgccgt cgccgcgcac gcctgcggtg gagaagctcg tgcagcgcaa gtggcgcgag
     2341 aagcgaacga tcgagggcgt gagcggcgag gtcatcctcg acgccgagaa gggcgtgccg
     2401 ctttcggtga agctcgcggg cacggtcggg ttcatgcggg acggtcgacg cttcgcgatg
     2461 aaggtgacgc tcgatgccgt gctcgacggt gtcggccccg tggatgtcca ggcgccaccc
     2521 gcggatcagg tcgtcgcgac acccgagcgc ctcaaagagg tggacgatcg cgacttcctc
     2581 ctgcagggca tcgccccgcc actccgtaag aatcccgacg gaaccgcagt accgccgcaa
     2641 ccgactcccg ctggctcggc gagtgggtca aactctggga aatgaccgcc ggtacggccc
     2701 tcgtccagcg ccgcgattac ctcgcgggac agcgtcgcac gatcatcgcg cgctcgttga
     2761 tcggcagcct cgcgggcgca ttgccgctgc cgttcctcga tgactgggcg gtcgcccgga
     2821 tcctcggcgg tggctacgag cgcatcgcgg ccgcgcacca cgtcgatctc catcccgacg
     2881 cggtgaacgc gctcgtgcat ggcgcgacga agcctccgac catcgcgaac atcgcgatcg
     2941 gtggcgtact ggcgcgcgtg gcgtcgttgg cggctcgccg catgatggtc gcgatcgccg
     3001 cgatcaatcg cgcgcggtcc gccgcccgga cgtacacggc gatgacgctg ttcgatcact
     3061 actgcgcgaa gctgcacacg ggcctcgagc tcgacgggcc aacggcgctg ctgctgcgcg
     3121 aagagatcat tcgctcgatc gatcagacac cgggcgcgct cgcatttcat ccgtttcgcc
     3181 gcgcggcgct gtccgctgca cgtgccaccg tgcgcgcgcc actcgagctt gtcgacctcg
     3241 tctcgggcgg gcggctgcgc aagatgctcg cagcgaagag tgaatcgtcg ggcgtcgccg
     3301 agccggagca catcgacgat atcgagctcg cgatggaagc ggccctcgcc gacaagaaca
     3361 acttcctcgc gcgcacggtc gcgtctgtgg aagcgcagct gtcgagcgag gtgaatccgt
     3421 tcctcgatgc agcgatcgaa tcgctcgatc gacggtggcg tgcgcgtgtg gcggcgaagc
     3481 gatgacccag tcgccggacg tccgccacga acgagcagct ctgaaggacg cgaagcgcgt
     3541 cgtcgtgaag atcggtagcc ggctcatcgc ggatgcgcct gcgagccgcc cggcgacgat
     3601 cgcggatcaa gttgcagagc ttcgtcgacg cggcgtcgag gtggtcatcg tctcgtccgg
     3661 tgcgatcgcg cttgggctgc cgcgcctcaa gatgaagcag cgcccgcacg agatgccgct
     3721 gctccaggcc gcagccgccg tcgggcaatc gcgcttgatg cagcagtggg agcacgcgtt
     3781 cggtgcgcac cagatcgaga tcggccaggt cctcgtcacg cacgacgacc tcggtgatcg
     3841 gcggcggttt ctctcggcgc gcctcacgct ccgcgcgttg ctcgatcacg gcgtcgtgcc
     3901 gatcatcaac gagaacgaca cggtcgcgag cgaggagatc aagttcggcg acaacgatca
     3961 gctcgccgcg ctcgtctgca atctggtttc cgcggacctc ctgatcatcc tcaccgacgt
     4021 cgaaggagtc cgagacgcgc atgggatccg catgccgatc gtccacgaca tcgatcgcga
     4081 agcggtgccc gtcgcgggtg gctcgacgag cggtgtcggc tccggcggca tggcgagcaa
     4141 ggttggctcg gcgaggatcg tcacgcgaac cggcattccg gctgtcgtcg cgcccggccg
     4201 cgaggcggaa gtcctcacgt gggtcctcgc cggtgtcgac gtcggaacgc tattcgttcc
     4261 gcccgcgcgc ggcgccggcg tgctctcggc gcgcaagcac tggattgcct acggggccaa
     4321 gccgtgcggc aagctcaccg tcgacgacgg cgcggtgaag gcgcttacga ccggcggcaa
     4381 gagcctgctt cccgccggaa tcattgccgt cgagggcgac ttcgagctcg gcgataccgt
     4441 gtcgatcgtg acccaaaacg gcaacgaaat cgcgcgtggt ctcgcagcgt atcccgggga
     4501 cgatttgcgg aagatccggg gcttgcaatc agcgggtatc gaggccacgc tgggttataa
     4561 gagcctcgac gaagcgatcc accgggatga tctcgtaatt ctgtgaccca gctcgaacgc
     4621 gaacagctca ttcgagtatc cgcgcaagcg gccaaggcgg catcgagagt cgtcgcgaag
     4681 gccccggcgg cgcagcggca gaaagctctc ctcgcgatcg cggatgcgat cgatgcgcag
     4741 gccccgcaga tcctcgctgc gaatgcgaag gacgtcgcga cggcatcggg tctctccgct
     4801 gcgatggtcg accgcctcac gctcgatgcg aagcgcgtag ccggtctctc tcgcgccgtt
     4861 cgcgaggtcg ccgcacaaga agaaatcctc ggccgcatcg acaagaccga tgcgcgtccg
     4921 aacggtctcg aaatttcgcg cgtgcgcatc ccgctcggtg tgatcgcgat gatctacgag
     4981 gcgcgcccga acgtcacgac cgacgccgca gcgctctgcc tcaaagccgg caacgcgtgc
     5041 atcctgcgcg gcggtcgcga agcgctcgaa agcaatcgcg ccctgctccg cgccgtccag
     5101 accggcctcg cgaccgcggg cctccccgcc gaagcggtgc agctcattcc cgtgatggaa
     5161 cgcgaagcga tcgcagacct cgtgaagctc gacgagctga tcgacctct
//
LOCUS       DATLBZ010000377         7935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6183716, whole genome shotgun sequence.
ACCESSION   DATLBZ010000377 DATLBZ010000000
VERSION     DATLBZ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6183716"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VMZ53_19265"
     CDS             <1..664
                     /locus_tag="VMZ53_19265"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HUS30662.1"
                     /translation="PEVVAGRYGWPLLSVILCACIAAFALGTRLDVAPDVRAENAGAA
                     TVDANSKSKPAPEIKTDREIDEAIAQRAAVVRVKLGLGAALGTPFRILALALAMLLLG
                     RFIGGKPTMPRALTVAALASVPGAVRSLVTAVVAWRQPSVLPGEVDSLVRFPTVIPEG
                     HPVLARLFDGVDFFTWWSVVILAFGLCAAAEMRRAKGFVAVAVSFALFLVVTRLIMGG
                     ER"
     gene            661..1893
                     /locus_tag="VMZ53_19270"
     CDS             661..1893
                     /locus_tag="VMZ53_19270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUS30663.1"
                     /translation="MTIARWIFAVVIIGLIGLVAMKSLQPRPERTTSVQSATATRTSI
                     TRTVSGAGKIEPVHKVNVSSNITGTLLELTVGIGSKVTKGQTIAQIDTSLYRAQAEQQ
                     RAQLQAAQAAVVGARGNVKYLTEEAERNKKLFARGVVSEAEVTKGQSSMDLAMSELSA
                     AESRASMSRAALAEAQNALNWATVKAPIDGTVLATNHRAGERVRGSDFAEDVILVLGS
                     LTEVDIRLEVGEHDVVYIQPGQTAVVEIDAFPGQQLSGTVIDSGRDAIVKNAGTENEV
                     TTFPVWVSLTMPPGRVLSGMSAQVTISTETHPNVIAVPIQAVTVRPAGEAGSAGSAGP
                     AGSASTAAKRKLDKVVFVIEGGVVHKRVVEVGLSSETHVEITKGLKEGEVVVEGPYRT
                     LARDLVDGMRISAEPVSR"
     gene            1890..2627
                     /locus_tag="VMZ53_19275"
     CDS             1890..2627
                     /locus_tag="VMZ53_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS30664.1"
                     /translation="MTEPQALLEISGVTRRYPMGAEIIHALREVSFTVVRGEYLAIVG
                     TSGSGKSTLLNILGCLDTPSSGMYRLRGADVRGLGDDALSDLRNREIGFVFQTFQLLP
                     RTSALENVELPLVYRGVGRRARRERARAALARVGLADRMRHRPNELSGGQRQRVAIAR
                     ALVTEPSILLADEPTGNLDSATGREILQLFTELHAAGNTLVLVTHEPTIAAQCPRAIR
                     MSDGRIVSDGPGAHVAAELTRGTAAVT"
     gene            2624..3862
                     /locus_tag="VMZ53_19280"
     CDS             2624..3862
                     /locus_tag="VMZ53_19280"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS30665.1"
                     /translation="MIVGIRRFGVEAAAAIMTALRGIVANRMRAFLSTIGIAIGVATL
                     MTIYGLVSGLTTSFTAQIAALGSNTMYVTDRPWIIRGDWWEYRNRPPITRADVAALHQ
                     DATYLAAVAPVASAAAEVSFRSERISGVQVRGTTSEYLDTSTLKLEQGRFLSALEGAS
                     DNQVVVVGSEVVERLFPNEPPLGAKILLGPHRFTVIGVLKPQGKAFGRSLDNIVIIPI
                     EAFGSIYGMQRDMAIVASAMPDHLYEAEEQIIEVLRRSRGLGPEVDDNFSINRQSELV
                     KIFNEETSALFGVAIAIGLITLLVGGIGVMNIMLVAVTERTREIGVRRALGARQRTIL
                     MQFLVESALVTMIGGSIGTALGVLASGVIDRVSPIGAELSTTAIVGAIVFSAVVGLAF
                     GTWPASRAASLDPIESLRFE"
     gene            3859..5091
                     /locus_tag="VMZ53_19285"
     CDS             3859..5091
                     /locus_tag="VMZ53_19285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS30666.1"
                     /translation="MIYSFADTLLSAIRTLRVNKLRSALTLLGIVIGVVAVVAMAATI
                     EGLRRKISSDLAQLGSGVFQVQKQPTGGFNDNDRAKFDKRKDFTLRDVDLLESQCASC
                     LAVGGETWEFGRDVSAPSHPAEITAASVGATAAFFDNNGYALANGRLFTEGEARSGMG
                     VVVIGADIVDLLFANESPLGNTVFIRGHRFRVIGTLTRRGEQLGGSLDNIVVVPIAAF
                     MPIYGSRRSLNVTVQARDPARIGRTQDEVTALLRRARGVPPEAENDFEMFSNASMQEM
                     FDKMSGIVAAASIGICAIALLIGGIGVMNIMLVSVTERTSEIGLRRALGARRRRILAQ
                     FIVEAVMLTSIGGVLGVLIGTGLASLIEVLVHIPTAVPMWAVVLSMAAAGGVGLVFGI
                     YPAYRASRLDPVEAMRRE"
     gene            complement(5094..6161)
                     /locus_tag="VMZ53_19290"
     CDS             complement(5094..6161)
                     /locus_tag="VMZ53_19290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS30667.1"
                     /translation="MTRFLLVGNSGSGKSTLAARLAAREGLPHLDLDTLAWLPTTPPS
                     RRPLADSERDIRAFTDANAAWVIEGCYADLLALVGADATLVFLNPGVDACIENARQRP
                     WEPHKYESKAAQDANLEMLIGWIRGYETRTDAMSLAAHRALFDTYAGDKVELTSREAI
                     AAYGARDADDESRPTDEIVAQLVASHREFLAFIERRVGDRALAEDILQDALVKNLHRV
                     DEIRDSAIGWFYRVLRNAIIDRARRANVQRARLDAFAHELEATATDETELRNEVCRCV
                     TTLASTLKPEYADALQRIDIDGVAVKDYAETAGISASNAGVRVFRAREALRKQVGRAC
                     GTCATHGCFDCTCGTSSAHPA"
     gene            complement(6223..6369)
                     /locus_tag="VMZ53_19295"
     CDS             complement(6223..6369)
                     /locus_tag="VMZ53_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30668.1"
                     /translation="MPLNKENVMTNQAKTQNQNNCGCQTGQRCTCGGRCSCKSCACGK
                     ECGK"
     gene            6479..6862
                     /locus_tag="VMZ53_19300"
     CDS             6479..6862
                     /locus_tag="VMZ53_19300"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HUS30669.1"
                     /translation="MTKPSPSTLTRREREIMDILYRRERATAAEVLEEMADPPSYSAV
                     RALLRILEDEGHIKHVQDGPRYVYLPAVARNDARKSALSHVVTTFFEGSVEQAVATLV
                     ERSKLSKDELDRLAQLIEKAKKEGR"
     gene            6862..>7935
                     /locus_tag="VMZ53_19305"
     CDS             6862..>7935
                     /locus_tag="VMZ53_19305"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HUS30670.1"
                     /translation="MTLTLIALAAVRAAVLLALVLTALRFMGRFSAATRRSLLVGAFT
                     IVLVLPIASAVLPALHLRGADTQVDPTPSVAKTAPDPIDEAVPMVDVPSVDARAEQTP
                     HAAVARETATSWSPLSIALGVWALGAVIVLARLGIGLARARRLVTSARFVETLDIGGR
                     PVQVRVSSTIETPAVTGLLVPVVLLPEEAATWSAERRRLVLAHELAHVTSRDCLANVI
                     AQLAVAVHWFDPLVWIAARRLRIERELAADDRVLDDGVLPSSYAEHLLALATNHGRSM
                     PQGALAMAEPAEVSLRVRALLAPHTRTPLGRNRFALAGAGLALAAVVACATPEREAAP
                     APTANEPAATAKPTTPVATASTTI"
BASE COUNT         1291 a         2617 c         2634 g         1393 t
ORIGIN      
        1 tccggaagtc gtcgcaggtc ggtacggatg gccactgctc agcgtgatct tgtgcgcgtg
       61 catcgctgcg tttgcgctcg ggacgcggct cgatgttgca cccgacgttc gtgcggagaa
      121 cgcgggcgcc gcgaccgtcg acgcgaacag caagtcgaag ccggcgcccg agatcaagac
      181 cgaccgcgag atcgacgaag cgatcgcgca gcgcgcggcg gtcgtgcgcg tgaagctcgg
      241 actcggcgcc gcgttgggga cgccgtttcg catcctcgcg ctcgccttgg cgatgctgtt
      301 gctcgggcgg tttatcggcg gcaagccgac gatgccgcgc gcgttgacgg tggcggcgtt
      361 ggcatcggtg cccggtgcgg tgcgatcgct cgtgacggcg gtcgtcgcgt ggcgccagcc
      421 gagcgtgctg cccggcgagg tcgactcgct ggtgcgattt ccgacggtga tcccagaggg
      481 ccatcccgtg ctcgcgcgct tgttcgacgg cgtcgatttc tttacgtggt ggtcggtcgt
      541 catcctcgca ttcgggttgt gcgccgctgc ggagatgagg cgcgccaaag gctttgtcgc
      601 ggttgcggtc agcttcgcgc tgttcctcgt cgtaacgcgt ttgattatgg gtggtgaacg
      661 atgaccattg cacgctggat ttttgcggtc gtgatcatcg gcctcatcgg gctggtggcc
      721 atgaagagcc tgcagccgcg accggaacgc acgacatcgg tgcaatcggc gacggcgaca
      781 cgaacctcga tcacgcggac cgtgagcggc gccggcaaga tcgagcccgt gcacaaggtg
      841 aacgtctcgt cgaacatcac gggcacgctg ctcgagctca cggtcggcat cggctcgaag
      901 gtgacgaagg gccagacgat cgcgcagatc gacacatcgc tgtatcgcgc gcaggcagag
      961 caacagcgcg cacagctgca ggcggcacaa gcggcggtcg tcggtgcgcg cgggaacgtg
     1021 aagtacctga cggaagaagc agagcgaaac aagaagctgt tcgctcgcgg cgtcgtcagc
     1081 gaagccgagg tcacgaaggg tcaatcgagc atggacctcg cgatgtcgga gctgtcggcc
     1141 gcagagagcc gtgcatcgat gagccgagcc gcgctggccg aagcgcagaa cgcgctgaac
     1201 tgggcgaccg tgaaggcgcc aatcgacgga accgtgctcg cgacgaatca ccgcgccggc
     1261 gagcgcgttc gcggcagtga cttcgccgag gacgtgatct tggtgctcgg ctcgctgacc
     1321 gaggtggaca tccggctcga ggtcggcgag cacgacgtgg tctatatcca accgggacag
     1381 accgcggtcg tcgagatcga tgcgtttccc ggccagcagc tcagcggaac cgtcatcgac
     1441 agcggccgcg atgcgatcgt gaagaacgcc ggcacggaga acgaggtcac gacgtttccg
     1501 gtctgggtct cgctgacgat gccaccgggt cgcgtgctgt cgggcatgag cgcgcaagtc
     1561 acgatctcga ccgagacgca tcccaatgtc atcgcggtcc cgatccaagc ggtgacagtg
     1621 cgccctgcgg gcgaagcggg ctccgccgga tctgcggggc ctgcaggctc ggcgtcgact
     1681 gcggcgaagc gcaagctcga caaggtcgtg ttcgtgatcg aaggcggcgt cgtgcacaag
     1741 cgcgtcgtcg aggtcgggct atcgtcggag acgcacgtcg agatcacgaa ggggctcaaa
     1801 gagggcgagg tcgtcgtcga gggtccgtat cgcacgctcg ctcgcgatct cgtcgatggc
     1861 atgcgcatct cggcggagcc agtctcgcga tgaccgagcc gcaggcgttg ctcgagatct
     1921 cgggcgtcac gcgccgctat ccgatgggcg cggagatcat tcacgcgttg cgcgaggtca
     1981 gctttaccgt cgtgcgcggc gagtacctcg cgatcgtcgg cacgtcgggc tcgggcaaga
     2041 gcacgctgct caacatcctc ggctgtctcg atacgccgag ctcgggcatg tatcggttgc
     2101 gtggcgcgga cgtgcgcggc cttggcgacg acgcgctctc ggatctgcgc aaccgcgaga
     2161 tcgggttcgt cttccagaca ttccagctgc tgccgcgcac gagcgcactc gagaacgtcg
     2221 agctgccgct tgtctatcgc ggcgttggca ggcgagcgcg gcgcgagcgt gcgcgtgcag
     2281 cgctagcgcg tgttggcctc gcagatcgca tgcggcatcg gccgaacgag ctatcgggcg
     2341 gccagcgcca gcgcgttgcc atcgcgagag cgctcgtcac cgagccatcg atcctgctcg
     2401 ccgacgaacc gaccggcaat ctcgattcag cgacgggtcg cgagatccta cagctgttca
     2461 ccgagctgca cgcggcgggc aacacgctcg tcctcgtcac gcacgaaccg acgattgcgg
     2521 cgcagtgccc gcgcgccatt cgcatgtcgg acggacgcat cgtctccgac ggtccaggcg
     2581 ctcacgtagc ggccgagctc acgcgaggca ccgcagcagt cacatgatcg tcggcatccg
     2641 cagattcggc gtcgaggcgg cggccgcgat catgaccgcg ctccgcggca tcgtcgcgaa
     2701 ccggatgcgc gcgttcctca gcacgatcgg catcgcgatc ggtgtcgcga cattgatgac
     2761 gatctacggg ctcgtctctg ggctcacgac gtcgttcacg gcgcagatcg cggcgctcgg
     2821 atcgaacacg atgtacgtga ccgaccgccc gtggatcatt cgcggtgact ggtgggagta
     2881 tcgcaatcgc ccgccgatca cgcgcgccga cgtggcagcg ctgcaccagg atgcgaccta
     2941 cttggccgcc gtcgcgccgg tcgcgtccgc tgcagccgaa gtttcgtttc gcagcgaacg
     3001 gatcagcggc gtgcaggttc gcggcacgac gagcgaatac ctcgacacct cgacgctcaa
     3061 gctcgagcag ggccgctttc tctcggcgct cgaaggtgcg agcgataacc aagtcgtcgt
     3121 cgtcggctcc gaagtggtcg agcggttgtt cccgaacgag ccgccgctcg gcgcgaagat
     3181 cttgcttggc ccgcaccgct tcacggtgat cggcgtgctc aagccgcagg gcaaagcgtt
     3241 cggccggtcc ctcgacaaca tcgtcatcat tccgatcgag gctttcggct cgatctacgg
     3301 catgcagcgc gacatggcga tcgtcgcgtc ggcgatgccc gaccacctgt atgaagccga
     3361 agaacagatc atcgaagtgc tccgccgttc gcgcggcctt ggccccgaag tcgacgacaa
     3421 cttttcgatc aaccgccagt ccgagctcgt gaagatcttc aacgaagaga cctcggcgct
     3481 gttcggcgtc gcgatcgcga tcggcctcat cacgctgctc gtcggcggca tcggcgtcat
     3541 gaacatcatg ttggtcgccg tcaccgaacg cacgcgagag atcggcgtac ggcgtgcgct
     3601 cggcgcgcgg cagcggacga tcctcatgca attcctcgtc gagtccgcgc tcgtcaccat
     3661 gatcggcggc agcatcggca ccgcgctcgg cgtgttggcg tccggcgtca tcgatcgcgt
     3721 ctcgccaatc ggcgccgagc tctcgacgac cgcgatcgtc ggcgcgatcg tgttctccgc
     3781 agtcgtcggc ctcgcattcg gaacgtggcc tgcatcgcgc gcagctagcc tcgatccgat
     3841 cgaatcgctg aggttcgagt gatctacagc ttcgcagaca cgctgctctc ggcgatccgc
     3901 acgctgcgcg tgaacaagct gcgctctgcc ctcacgctgc tcggcatcgt gatcggcgtt
     3961 gtcgcggtcg tcgcgatggc ggcaacgatc gaggggctgc gccgcaagat ctcgagcgac
     4021 ctcgcgcagc tcggctcggg cgtattccaa gtccagaagc agccgaccgg cggtttcaac
     4081 gacaacgacc gcgcgaagtt cgacaagcgc aaagacttca cgctgcgcga cgtcgacttg
     4141 ctcgagagcc agtgtgcgag ctgcctcgcg gtcggcggtg agacgtggga attcggccgc
     4201 gatgtctccg cgccgagcca cccggcagaa atcacggctg cgagcgtcgg cgccacagcg
     4261 gcattcttcg acaacaacgg ctatgcgctc gcgaacggcc gcttgttcac cgagggcgaa
     4321 gcgcgaagcg gcatgggcgt cgttgtcatc ggcgccgaca tcgtcgacct cttgttcgcc
     4381 aacgagtcgc cgctcggcaa cacggtgttc atccgcggcc accgctttcg cgtcatcgga
     4441 acgctgaccc gccgcggcga acaactcggc ggctcgctcg acaacatcgt cgtcgttcca
     4501 atcgccgcgt tcatgccgat ctacggcagc cggcgttcgc tcaacgtcac ggtgcaggcg
     4561 cgagatcccg cgcgaatcgg ccgcacgcaa gacgaagtca ccgcactgtt gcgacgcgct
     4621 cgcggcgtcc cgcccgaggc cgagaacgac ttcgagatgt tctccaacgc atcgatgcaa
     4681 gagatgttcg acaagatgag cggcatcgtc gccgccgcat cgatcggcat ctgcgcgatc
     4741 gcgctgttga tcggcggcat cggcgtgatg aacatcatgc tggtctcggt caccgagcgg
     4801 accagcgaga tcggattgcg acgtgcgcta ggcgctcgcc gccgccgaat cctcgcgcag
     4861 ttcatcgtcg aggcggtgat gctcaccagc attggtggcg tgctcggcgt tctgatcggc
     4921 acaggcctcg cctccttgat cgaagtgctc gtgcacattc cgacggcggt cccgatgtgg
     4981 gccgtcgtgc tgtcgatggc ggccgccggc ggcgttggtc tcgtcttcgg catctatccg
     5041 gcgtaccgcg cctcgcgact cgatccggtc gaggcgatgc gccgcgagta gcgtcacgcg
     5101 ggatgcgcag acgacgtccc gcacgtgcag tcgaagcagc cgtgcgtcgc acacgttccg
     5161 catgcgcgac caacctgctt gcgcagcgcc tcccgcgcgc gaaacacgcg gacaccggca
     5221 ttgctcgccg agatgccagc ggtctccgcg tagtccttca cggcgacgcc atcgatgtcg
     5281 atccgctgca gcgcatcggc gtactcgggc tttagcgtgc tcgcgagcgt ggtgacgcag
     5341 cggcagacct cgttgcgaag ctccgtctcg tcggtcgccg ttgcctcgag ctcgtgcgcg
     5401 aacgcgtcga ggcgagcgcg ctggacgttt gcccgacgcg cgcggtcgat gatcgcgttg
     5461 cgcagcacgc ggtagaacca gccgatcgcg gagtcgcgaa tctcgtccac gcgatggagg
     5521 ttcttcacga gtgcgtcctg caggatgtct tctgcgagcg cgcggtcgcc gacacggcgc
     5581 tcgatgaacg cgagaaactc gcgatggctt gcgacgagct gcgcgacaat ctcgtccgtc
     5641 ggcctcgatt catcatcggc atcgcgcgcg ccatatgccg cgatcgcttc gcgcgatgtg
     5701 agctcgacct tgtcaccggc gtacgtgtcg aacagcgcgc gatgcgcggc aagcgacatc
     5761 gcatcggttc gcgtctcata tccgcggatc cagccgatca gcatctcgag gttcgcgtct
     5821 tgtgcagcct tcgattcgta cttgtgcggt tcccacgggc gctgccgggc gttctcgata
     5881 cacgcgtcga cgccggggtt gagaaagacc agcgtcgcgt cggcaccgac gagcgccagc
     5941 agatctgcgt agcagccttc gatcacccac gctgcatttg cgtccgtgaa ggcacggatg
     6001 tcgcgttcgc tgtcagcaag cgggcggcgc gacggcgggg tggtcgggag ccaggcgagc
     6061 gtgtccagat cgagatgcgg aaggccctca cgtgcggcca ggcgcgctgc gagcgtggac
     6121 ttgccggagc ccgagtttcc gacgaggaga aaccgcgtca tctgcatact ttggtgccgc
     6181 atgcccatat gacgcaacgt cctggccggc attacacacc agctacttgc cgcactcctt
     6241 gccgcacgcg cagctcttgc acgaacagcg tcctccgcac gtgcagcgct ggcccgtctg
     6301 gcaaccacag ttgttctggt tttgagtctt tgcttggttc gtcatcacgt tctccttgtt
     6361 gagaggcatt tggtcgcctc gctcaggaga cgcagccggc cgcgccgcat tacacagcgc
     6421 taaattttta gcagataatg tttgctagat ttttagcagg ctgctaattc tctagcacat
     6481 gacgaagccc tccccgtcta cgctcacgcg ccgcgagcgc gagatcatgg acatcctcta
     6541 ccggcgagag cgggcgaccg cggccgaggt cctcgaagag atggccgacc cgcccagcta
     6601 ctcggcggta cgcgccctgc tccgcattct cgaggacgaa gggcacatca agcacgtgca
     6661 ggatgggccg cgctacgtct atctcccagc ggtcgctcgc aacgatgctc gcaagagcgc
     6721 gctgtcgcac gtcgtcacca cattcttcga gggctctgtc gagcaagccg tcgcgacgct
     6781 cgtcgagcgt tcgaagctgt cgaaagatga gctcgaccgc ctcgcacagt tgatcgagaa
     6841 ggcgaagaag gagggacgct aatgacgctc acgttgatcg ctctcgccgc ggtacgtgcg
     6901 gccgtccttc tggctctcgt gctgaccgcg ctgcgcttca tgggccggtt ctccgcggcg
     6961 acacgccggt cactgctcgt cggcgcgttc acgatcgtgc tcgtgctgcc gatcgcgagt
     7021 gcggtcttgc ctgcgcttca tctgcgcggc gccgatacgc aggttgatcc gacgccgtct
     7081 gtcgcgaaga ctgcacccga cccgatcgac gaggcggtgc cgatggtcga tgtgcccagc
     7141 gttgatgcgc gcgccgagca gacaccgcat gcggccgtgg cgcgagagac cgcgacgagc
     7201 tggtcgcctc tgtcgatcgc ccttggcgtg tgggcgctcg gtgcagtcat cgtgctcgcg
     7261 cgcctgggca tcgggctcgc tcgtgcgcgt cgcctcgtca cgtcggcgag attcgtcgag
     7321 acgctcgaca tcggtgggcg ccccgtacag gttcgcgtca gctcgacgat cgagacgccc
     7381 gcggtgaccg gcttgctggt tcccgtcgtg ctgcttcccg aagaggcggc gacatggagc
     7441 gccgaacgcc gccggctcgt cctcgcccac gagctcgcgc acgtaacgag ccgcgactgt
     7501 ctcgcgaacg tcatcgcgca gcttgcagtc gcggtgcatt ggttcgatcc gcttgtctgg
     7561 atcgccgcgc ggaggctgcg catcgagcgc gagctcgccg cggacgatcg cgtcctcgac
     7621 gatggcgtgc tgccttcgag ttacgccgag cacctgctcg cgctcgcgac caaccacggt
     7681 cgctcgatgc cgcaaggcgc gctcgcgatg gcagagccgg cagaggtctc gctgcgcgtt
     7741 cgcgcgctgc tcgcaccgca cacgcggacg ccgctcggtc gcaaccgctt cgcactcgcg
     7801 ggcgcagggc tcgcgctcgc cgcggtcgtc gcgtgtgcaa caccggaacg cgaagctgca
     7861 cccgctccca cggcgaacga gcctgcagcg acggcgaaac cgacgacacc tgtcgcgacg
     7921 gcatccacga ccatc
//
LOCUS       DATLBZ010000378        23551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2680790, whole genome shotgun sequence.
ACCESSION   DATLBZ010000378 DATLBZ010000000
VERSION     DATLBZ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 23551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23551
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2680790"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VMZ53_19310"
     CDS             <1..565
                     /locus_tag="VMZ53_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30671.1"
                     /translation="YHWMMAPVRKLSNGSYQYLNATAMAFPASAGRVNILFGYGSNVH
                     CMGDDAAFGRLFYSLKPRGWRSDGGKAMRGDLLGGWRLNVGTGTHSASRIFVFRDDGQ
                     YVDTIQEVSQLAIWETTRTRTGDGTWALSDDGVLTLTPQGQTGEKRRVRVYDEISPGG
                     MTWRKMRMIGVGSTEYREVELDRISWK"
     gene            complement(567..2255)
                     /locus_tag="VMZ53_19315"
     CDS             complement(567..2255)
                     /locus_tag="VMZ53_19315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30672.1"
                     /translation="MLGGGVRDERAVAATVTASTQISLVRRAFLPSPLETRPGPAQID
                     AFARLRGWFTLSAIVVDIAIYFGFRGSPRFDADALRGFAITNIPLLLISCVLCFFVLR
                     KPRRLHLPLSYLCVAIETFTSLTWIQLTGSVSSYFLIVIPVLILAYRLYAGYRLGISV
                     YLFGAVLHAVMVALEELHVLEPASLFISDPGAIYREPLFRAAAASSIQLMFLAIFVLA
                     NLVSRALREKENQLDVVQRNLDRVVADVQPGRLSGQTLDGKYRVNELLGRGGMGEVYQ
                     AARLADGRDVAVKVLYAHLGTPDLLERFRREAAIASKLPATNVAQVLDVGHARDGGHH
                     YLVMELLQGEDLGMLLRRRGRLPAEELLPIIDQLVGVIEAAHASGIVHRDLKPQNIFL
                     VASEGGAPLVKLLDFGVARLVEGSELTQSAMLIGSPGYLAPEQVATEFGEVGPRADVF
                     ALGAITYRALTGQSAFPARNAAAAVYEAVHRDPDRPSKLDASLHADLDAFMVLALAKQ
                     PAKRYASATELARDLRAACAGTLDEATRARARDSDRKRDSSPAMAPTLAESLAP"
     gene            2371..3309
                     /locus_tag="VMZ53_19320"
     CDS             2371..3309
                     /locus_tag="VMZ53_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30673.1"
                     /translation="MLVAAALTGCLADGGGVDGDQTEQESTTTYVDILDFSQTDQSQW
                     YDLIHNLNHQFDAVCGDTFCEGEYSNIQPLTFSCSVSSKAGSVKDCAWTFAAAQVDVD
                     PRTAAIVVDAPTFQCHFQMKTTAKRLVGILAGADPLHAALPGAPAATPSIYDQLSACF
                     QHPIGQTPGTFAETGSLSYVSARDYYTSSAGIERWAAGKAALKDGFDRVCGDTFCSGD
                     FGDLQAMDFTCAITKSTGNVKSCMWTFGGSWHLVAEHGGAQQITQSTFKCPVLMKGTL
                     SKFLDVLLAPGTDQPINRPLPGMTTSAYEALLGCLP"
     gene            3417..5141
                     /locus_tag="VMZ53_19325"
     CDS             3417..5141
                     /locus_tag="VMZ53_19325"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS30674.1"
                     /translation="MRAWRWLSAASLLLPGIGNIFVHYVPPLAIAHLLATLARDKHPS
                     TGELATPVILLAVAWFGGEAIWRFASFLLSRLEYHAMRALYNEAMDELFVKDVGFFHN
                     NFAGSLTKRVLGYARRFEDVFDVFAFSIVGNLFPLTFAIIVLGKFSGWLILVLLVMLT
                     FAFFCMRPLIRKRHALVQIREQASNVLAGHVADSITNAETVRAFAREPEEASIHAKNV
                     DDFTAKMLRAWDYQNTRVDIVTSPIYVFTNIAGLVVALVIGRNSGFEIEAVFLTFSYF
                     AQSTRVMWEFNRIYRNLETSLTDAAQFTELLLEPPAIQDAERVEAFEPQDSSVRFRSI
                     RFAYPGRPPLFDNFDLTIAPGEKVGLVGRSGGGKTTITRLLLRFADVQAGAIEIGGQN
                     IALIAQSALRDAIAVVPQEPAMFHRTIADNIRFGRPNATDDEVRRAAELAHADEFIAA
                     LPAGFETLVGERGIKLSGGQRQRIAIARAILKDAPILVLDEATSALDSESESHIQDAL
                     RTLMKDRTAIVIAHRLSTVRRMDRLVVLEEGRVIEQGRHDQLLAMAGQYAALWEHQSG
                     GFMQERAV"
     gene            5225..5896
                     /locus_tag="VMZ53_19330"
     CDS             5225..5896
                     /locus_tag="VMZ53_19330"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUS30675.1"
                     /translation="MPTQLLNDDGTASMATMLMSSHHAFRRDAACFALALADGSRPAA
                     VLAEEWARFRGALHGHHTIEDTAMFPDLRAKHPELGATLDKLDAHHRAIDPLLERGDK
                     LFADLGTQRGAASDLIATLQGLLKEHLDLEEHAITPHLRAAKEFPLPPTEDAAAMYAE
                     GFAWSMAGISDAVLGKMREMLPAALVEKLPAARTTFDERCRRVWGYAHAGASTTSDPA
                     ATART"
     gene            complement(5880..6425)
                     /locus_tag="VMZ53_19335"
     CDS             complement(5880..6425)
                     /locus_tag="VMZ53_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30676.1"
                     /translation="MRHLPIAIVLATAACGSSEDLTCELLADPTNCWASAAERAAACL
                     PSRATPATLSADRKHCTWPDGAQVTFDTPLPNDTIDLERLAFDVTGPGGCAWRFVDTF
                     QNRMELTVDGKTEVSQLHPDREFELACSSGTSYTSSFDLLFTCQAPARAPTDGFDVTA
                     TSFEFTLSAVNAPTPLFTCAL"
     gene            6464..6802
                     /locus_tag="VMZ53_19340"
     CDS             6464..6802
                     /locus_tag="VMZ53_19340"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUS30677.1"
                     /translation="MVNYLFVASLDTTFSAISDPTRRAILDRLSRGPARVTDVAEPFA
                     MSLAAVSKHIKVLEDAGLVRRAKNGREHTLELEAAPLREAAQWTSGYQQFWTERLDRL
                     EAFFKQRKQS"
     gene            6799..7236
                     /locus_tag="VMZ53_19345"
     CDS             6799..7236
                     /locus_tag="VMZ53_19345"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HUS30678.1"
                     /translation="MKLTDLEVTRQIPATPEEVYDVWIDPTSPGGPWFSPQADGMSSK
                     ILFTAAVDGLFYHCVTNKGQAWAHYGRFIKLERGKLAEHTWVGEWTKGRESVVTTTFQ
                     AKDGGTLVTLRHTGVPDDEEGRRHAQGWAWCLSMLADKMGSKK"
     gene            7415..8803
                     /locus_tag="VMZ53_19350"
     CDS             7415..8803
                     /locus_tag="VMZ53_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30679.1"
                     /translation="MNRAELETTAPIKREELLGLLNAMTPAEQQAITARASISAIEAE
                     LARQREASQPSQPSQAEAEAEAVAEAPPRRRSRKATKAEKRAARKAARKAARAASRAQ
                     TQRAGDAESTNESIDESIDEAIDESTDASNDESIAAIAMATPANTNVATTAAANTNIA
                     PTATSGQATDATGDIAMSDAATGAAADSAAGDTSGTSGAAADSAAGDTSGTSGAAADS
                     AAGDTSSTSGAAADSAAGDNKLFEDIDWDNLLTNAVSSITEPKPSRATPPPIPAPASD
                     RPKRARTVRPFAQRAAMLPICEPKRFPRATQTPPYVRAAAHNTSVAAEEKAARVVAEV
                     RARVGSEHDMAEGSGPCDAIDRISDGVPCPVLSRRIVASLSGEEIAMLPMELRLALPK
                     TPTWENPPRIQMTPVPDGECITIHSTKPKDSCAAMSAGAPEIASVPSMATIVASFAAT
                     VLVGLAAFYFIF"
     gene            8875..12876
                     /locus_tag="VMZ53_19355"
     CDS             8875..12876
                     /locus_tag="VMZ53_19355"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30680.1"
                     /translation="MLQPGAIVAGRFEIEELAGRGGMGAVYRARDRQTGSVVALKVVH
                     EEAPRDHHERLVREARLLAELQHPAIVKYLAFGATDRGWPYLAMEWLEGEDLALHLRG
                     EGLDPSDAVTIALRVAEALGIAHERGIVHRDIKPSNLFLVGGDPARVKVLDFGVARIE
                     LADDGVTRTGAAVGTPRYMSPEQARGSRDVDVRADVFALGCVLFECLAGRPAFSGANQ
                     GAVLAKILLEEAPRLTSVRDDLPPALDELLARMLAKQRDDRPRDGRAVAAELAAIGDV
                     GTTARRTAGPRDAGVTTGEQRLVCVVFAETALEVSAGFEERTPSTPSTSFAWPVKPVA
                     GSDEETATLDPRRSVRPTHQVVRRLETIVAEHQGRAEVMKDGSVVVTLSGRSAATDQA
                     ASAARCALALRKIFPNTAMALAIGRSAVSDRYPLGDLIDRATAVLSRPPPEAVRHGTF
                     PIYVDDVSAALLDLRFSIEGDANGLYLSRERDVAVAARTLLGKPTECVGRERELGMLL
                     ATFEECVAEPMAHVVLVTAAAGIGKSRVRFELMQRLAQTGRPFELLLGRGDPMSAGTP
                     FAMLAQALRRAAGVVDDEPATVRYRKLRARVLRNVPPRDAEHVTEFLAELLGAGPPGE
                     GSVQLRAARQDPQLMGDQMLRACQQFFAAECDANPVVLVLEDLQWSDRTTVQFVDAIV
                     RQLAEKPLFVLALARPEVDAVFPRLWADRRVERLRLHELTRRASEKLVRETLGDVNPA
                     TVARIIDRAAGNAFYLEELIRTVAEGHEDRLPETVVAMVQARIESLEVEARHVLRAAS
                     IFGQVFWRGGVEALLEGGTTQVDDWLDELARRELVTVRRESRFSGEAEYTFRHAIVRE
                     AAYAMVTERDRMVGHRLAGAWLEARVATEGADIEAVALAEHFLRGGEPARSVTWYRHA
                     AQQALEGDDLSGAIDRAERAITCMDDADPTVRDRALVGTLRSLQAGAHVWRGEYAIAA
                     ERGNEALAVLSPASVAWLSAAAAVADACSRRLEHPWVLELCRRLAALEVTPNLYHAYA
                     HAVATTMTTLLWHGDADLSGRLFAQLARIEADRPGPDTLAWIYTAQAWRALHAGDHAG
                     ALALDQKVERCFTEVGDLRQACRQQASVGYGQLMLGANVEAEQSLRQSIAIATRIGLH
                     QVTTQAKHNLGICLARIGKLEEARDVERDALASFEAQENRRLCAFARFYLAEIELTAG
                     NAAGALAYAREALVVDAEQPSFHCVYRARIANAHLLAGDSAAALDEAKQAMELMERHG
                     RPEEGEMVVRLAYARALHATGDAATAREVITDIEWRVTTEAARIGDPALRRSFLEGVP
                     EHAVALSLAREWT"
     gene            complement(12873..13430)
                     /locus_tag="VMZ53_19360"
     CDS             complement(12873..13430)
                     /locus_tag="VMZ53_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30681.1"
                     /translation="MQFRAELAAVAPADRDAWLDARLGLGDLPDDGADLPRDCVPYLP
                     CSVDRLLRVIERAAVGPSDVFVDVGSGVGRAAAFVHLATGARAIGIEIQPALVAASRG
                     LRDAMSTIEADASRAGTELPLGTVYFLYCPFSGARLSRFLDNLEPIARAQPLRICCLD
                     MSLPERPWLEPVPQVDADLTIYRTL"
     gene            13509..13874
                     /locus_tag="VMZ53_19365"
     CDS             13509..13874
                     /locus_tag="VMZ53_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30682.1"
                     /translation="MKQTIGSILLAVALCACGKGGGGDIDTFMKLDSDKAAAFAAGGD
                     DCEAKAKSVGDWRKAHTAEYKALQKKLNDQWSSGPPKDVQDKYGDKMKANKKAVIDAM
                     FKCTDNEAFGKMMDETKTD"
     gene            complement(13879..14781)
                     /locus_tag="VMZ53_19370"
     CDS             complement(13879..14781)
                     /locus_tag="VMZ53_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30683.1"
                     /translation="MRAAVFVVVVASACGPTSRDGETADAAGSNTPQPDAGECKEVID
                     VVFVLDTSSSMDFVLTKLDAQIADVVTASNMVAADSHFGLIAFQDNYALDKTGSMMSI
                     HTSATSLQTAFRNYRDNYTAYNRNPGDGPSGQPTQNRICEENSLDSLYAAANEFPWRS
                     NATRIVILATDDTFLEASDNYGDRDGDGQTNKTDFPREGNYPAARTMANTVASLRMNK
                     IRVFSFSRITQPSFLDRCGTGRRLPWASITDGWSTPYKSQPPIPTQTDGGNYDLDKIK
                     AGTLSLSATINEVVLDSHCNPIIL"
     gene            complement(14857..15453)
                     /locus_tag="VMZ53_19375"
     CDS             complement(14857..15453)
                     /locus_tag="VMZ53_19375"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HUS30684.1"
                     /translation="MADSDSPDFDAVAAAKELDDLLGGTDTDTTAEADYVASLEDDVE
                     ALNAAIAKKDAELRAANQRADQAHAEIEAAGKRMANASAKELEQKSRKMLESFLEVVD
                     DLDRAIAAAKGTAAKDLVEGIELVRRNMLTRLGQFGVVHAPARGEPFDPKRHDAVALV
                     PVTDPAQDGRVIDVMREGYLVGEETLRPASVAVGKRNH"
     gene            15486..16028
                     /locus_tag="VMZ53_19380"
     CDS             15486..16028
                     /locus_tag="VMZ53_19380"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HUS30685.1"
                     /translation="MSLLDRLVSYAEYHRDKRNIATHFVGIPMIVIGTQSALARIGIG
                     PINVAAAVTGLATRYYRAIDPSYGAAMGAILTATYGIGTMLAAMPLPIWAGTTAGLFF
                     GGWALQFIGHFFEGKKPAFMDDLKGLLDGPLFLVAETAFALGFSKELRAEVERRAGPT
                     RWGKVAEATAPAFAHAAAAN"
     gene            complement(16025..17188)
                     /locus_tag="VMZ53_19385"
     CDS             complement(16025..17188)
                     /locus_tag="VMZ53_19385"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HUS30686.1"
                     /translation="MRSLFCSLLLLAACTSSPGDDMTGDDGPATCGNNSVDTGEQCDD
                     GNDNNFDGCRSDCTMVDKLTADPMAWRYFEIPGTKCIDGTPAGFSVNYNPASTKIAIY
                     LEGGGACFNTFCDSLFSRGGNQPSAGGIFDRANAANPLKDWTWVYVPYCSGDVYSGQA
                     DTMVGGKMRSFYGYSNMTAFLERIVPSFTVDQALLTGASAGGFGAAVNYTQTQRAYGS
                     IPVTLIDDSGPPMSADVYPPCLQTLWKTTWGLDKTVVKECGADCSGATFVEETFDHMR
                     QEFPNMKGGLFSSTGDQTIRTFAGYGWSGGYNMCKDTPSAATTTVYTAGLNALRTKVQ
                     GEGGNFGTYYISGSSHTILRSANFYTTQAGNVMLTKWIGDTLAGTPAHVGPQN"
     gene            17285..18286
                     /locus_tag="VMZ53_19390"
     CDS             17285..18286
                     /locus_tag="VMZ53_19390"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HUS30687.1"
                     /translation="MLTYSTSFCRILTDYLVSCGASVEPVIAAMGVDAAALEDRDGRI
                     PRRAWLHAFEVAVDVTGDPDLGLRVGESIRPAHLGALGYILMSCETMKQTAQVESRWH
                     SLVADGERLEYVREGQLSRRTLILPEGEPPPPACAAACAAAASVSFVRWLAGVGGDDG
                     LRRVELPYREPSSRAGHDRFFRCELVFDAPHVTVWRDPAALRKPLAQADPSLRARMEE
                     RATKLAAERSASDQLVTRVRELVVRDIRETVPDLEAVAAALELPPRVLKKRLADKATS
                     YTKIVDDTRRQLALGYIADPTLTLVDVAYLCGFSEQSAFNRAFKRWTGLPPGNYRRG"
     gene            18363..19412
                     /locus_tag="VMZ53_19395"
     CDS             18363..19412
                     /locus_tag="VMZ53_19395"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HUS30688.1"
                     /translation="MASQAELRELRGELRDAGLFEHCELRSWLKLGVLVGGVAASLVT
                     MATFGAWTALFAIPIAAVFATAAAMVAHEGSHRSFSASPTRNALMVYMVFPLFTGLGS
                     LYWRHKHDRLHHGHPNVEGVDPDIKPFPFVSSRGDHEKCGPKQRWFVSKLQRWAFWPM
                     STLLAVGMRRSSFLYLIRYPREHGIKAAWCVEALCMTGHYVLWYVIPCFIWGPVATLL
                     VNAGIWGLVGVMLTLVFAPAHIGLPVLVEPHHDWQHQLETTRNLQLPRVISFFFIGLD
                     YQVEHHLFPKIPHQKLPAAAAITARWCKKHGVPYQSVPYMTALKDSAQFMADAWTRDA
                     EYASVVRARMLDAVG"
     gene            complement(19409..20623)
                     /gene="cfa"
                     /locus_tag="VMZ53_19400"
     CDS             complement(19409..20623)
                     /gene="cfa"
                     /locus_tag="VMZ53_19400"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HUS30689.1"
                     /translation="MQHAQAPQRTTPRELLSQLWPLKTLTATRSADAEAIIRELFAHA
                     GIEIGGTNVGDIRVSDQRFYERVLRDASVGFGEAYMDDWWETDALDVTIDKIMRANLK
                     QKITGSWRMRALTVKAVVLNLQAKSRSGANVEAHYDIGNDLYTRMLDERMVYTCGYWK
                     DAKTLTEAQEAKLDLVCRKVGMKPGMRVLDLGCGWGGLASWAAEKYGCSVLGVTLSHD
                     QVSLGNQLWGPNGKKLDVELRLCDYRDVQGKFDRVVSIGMMEHVGPKNHRMMMETIDR
                     CLVDDGVALVHTIANNRSLRHGTPFIEKYIFPNAVAPSLAQIGRAIEGVFVMEDLHNI
                     GPDYDPTLMAWWENFDRTYSEISHRYDRRFYQMWKFYLLAAAGASRSRDGQLYQMVLT
                     KTGRKQPDCRAS"
     gene            complement(20683..20949)
                     /locus_tag="VMZ53_19405"
     CDS             complement(20683..20949)
                     /locus_tag="VMZ53_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30690.1"
                     /translation="MTEIVQPEMHPRAIAVGEAWASELVRSLRADDRQIIGSWPGTLG
                     EARMRIRGAFRLKLELDVIEELARLAYVAARRSWQELSEPDTEV"
     gene            complement(20942..21652)
                     /locus_tag="VMZ53_19410"
     CDS             complement(20942..21652)
                     /locus_tag="VMZ53_19410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS30691.1"
                     /translation="MTERQAERRRHERIAVKGTLIVHGQDQNQRGRIANISLGGVYVV
                     TKVALPDRMLARTIEFELRLDAGHAEWTRAAGRVSRLRPNGIAIAFDDIPPTLRKVIL
                     DISSSAHARARILSVVLVDADQPRRNAMADGFRSAGCHVIEASQPLEAIVRLGESSFE
                     PDVVAVADSRPTGAAEDMRKFVAAYHPSARLITIGDEPLQPDGLAHWLSSLNPDDDLA
                     RRVRAALIGPKSRGGAGD"
     gene            complement(22145..22681)
                     /locus_tag="VMZ53_19415"
     CDS             complement(22145..22681)
                     /locus_tag="VMZ53_19415"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HUS30692.1"
                     /translation="MKHADDVLDFWFGLTHTDWFQGSKELDAQIRDKFSATHDAVARG
                     ETFGWRATPEGRLAEVIVLDQFSRNIFRGSSQAFAYDGMALVLSQEAVAQKVDTKLEG
                     PRRTFLYMPYMHSESLVVHEEAMRLFTVLGDENALKFERIHIDTIRRFGRYPRRNEAL
                     GRTSTAEELEYMKSGGQF"
     gene            22736..>23551
                     /locus_tag="VMZ53_19420"
     CDS             22736..>23551
                     /locus_tag="VMZ53_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30693.1"
                     /translation="MRFAAVSLSIALLACGDDSSTTAPDAPMADTPTTCTRTPAAADR
                     TRFVVVAHPYTRSGDPSADFEVLQLSATGTLTRFAPPHTFTLGKRAPFGVITFTPDGV
                     VGIVALDDGKLGVFSLDGDGNATVIDAGVAGEFYADSVVMDPSGDHAWVLDANTRENG
                     GGIYRISIDCDGKVADKGIVAMAKTPGGLAFHDGHAVVPAHDILGSPTTGDDVHLLDW
                     PATAAAPTLLGGGDAFGDDDSIFSGFALSHDGATAFIGDSNVVGTNRVGIVSVS"
BASE COUNT         3968 a         7624 c         7977 g         3982 t
ORIGIN      
        1 gtatcactgg atgatggcgc cggtgcgcaa gctcagtaac ggcagctatc agtatctcaa
       61 cgcgacggcg atggcgtttc cggcgagcgc gggtcgcgtc aacattctgt tcggctacgg
      121 cagcaacgtg cactgcatgg gcgacgacgc cgcgtttggg cggctgttct acagcttgaa
      181 gccgcgaggt tggcgctccg atggcggcaa ggcaatgcgc ggcgatctgc tcggcggctg
      241 gcggttgaac gtaggcaccg gcacgcacag cgcgtcgcgg atcttcgtgt ttcgcgatga
      301 cggccagtac gtcgatacga tccaagaggt ctcgcagctc gccatctggg agacgacgcg
      361 cacgcgcacc ggcgacggca cgtgggccct ctccgatgac ggcgtcttga cgctcacccc
      421 gcagggccag accggcgaga agcgccgcgt gcgcgtctac gacgagattt cgccgggcgg
      481 catgacgtgg cgcaagatgc ggatgatcgg cgtgggcagc acggagtatc gcgaggtcga
      541 gctcgaccga atctcgtgga agtagcttag ggcgcgaggc tctcggcgag cgtaggtgcc
      601 atcgccggcg acgagtcgcg tttgcgatcg gaatcgcgag cgcgcgcacg cgttgcctcg
      661 tcgagcgtac cggcgcatgc ggcgcgtaag tcgcgagcca gctcggtcgc ggatgcgtag
      721 cgtttggcgg gttgcttcgc gagcgcgagc accatgaatg cgtcgaggtc ggcgtgcagc
      781 gacgcatcga gcttcgatgg gcgatccgga tcgcggtgca ccgcttcgta gacggcagcc
      841 gccgcgttgc gcgctgggaa tgcactttga ccggtgagcg cgcgatatgt gatcgcgccg
      901 agcgcgaaga catcggcgcg tgggccgacc tcgccgaact ccgtcgcgac ttgctcgggc
      961 gcgagatagc cgggcgagcc gatcagcatc gcgctctgcg tgagctcgct gccttcgacg
     1021 agccgagcga cgccgaagtc gagcagtttc acgagcggcg cgccaccctc gctcgccacg
     1081 aggaagatgt tctgcggctt gagatcgcga tggacaatgc ctgacgcatg cgccgcctcg
     1141 atcacaccga cgagctgatc gatgatcggc agcagctcct cggcgggcag acggccgcgg
     1201 cgccgcagca gcatgccgag gtcttcgcct tggaggagct ccatgacgag gtagtggtga
     1261 ccaccgtcgc gtgcgtggcc gacgtcgagg acttgcgcga catttgtcgc gggcagcttc
     1321 gacgcgatcg cggcttcgcg gcggaaccgc tcgaggagat cgggcgtgcc gagatgcgcg
     1381 tagagaactt tcacggcgac atcacgaccg tcggcgaggc gcgccgcttg atagacctcg
     1441 cccatgccgc cgcgcccgag cagctcgttc acgcgatact taccgtcgag agtctgaccg
     1501 gacaggcggc cgggctggac gtcggcgacg actcgatcga ggttgcgctg cacgacgtcg
     1561 agctggttct ccttctcgcg aagcgcgcgc gacacgagat ttgcgagcac gaagatcgcg
     1621 aggaacatga gctggatcga gctcgccgcg gccgcgcgaa acagtggctc tcgatagatc
     1681 gcgccgggat ccgagatgaa gagggatgcg ggctcgagca cgtgcagctc ttcgagcgcg
     1741 accatcaccg catggagcac ggcgccgaac agatagacgc tgatgccgag gcgatagccg
     1801 gcgtagagcc ggtacgcgag aatcagcacg gggatcacga tgaggaagta gctggagacc
     1861 gaacccgtga gctggatcca cgtcaggctc gtgaacgtct cgatcgcgac gcacagatag
     1921 gacagcggca ggtgcagccg cctcggcttg cgcagcacga agaaacacag cacgcacgag
     1981 atcagcagca gcgggatgtt cgtgatcgcg aagccgcgca gcgcatcagc gtcgaaccgc
     2041 ggcgagccgc gaaagccgaa atagatcgcg atgtcgacga cgatcgcgga cagcgtgaac
     2101 catccgcgta ggcgcgcgaa cgcatcgatc tgagccgggc cgggccgggt ttcgagcggc
     2161 gaggggagga atgcgcgccg gacgagcgag atttgcgtcg acgcggtcac cgtcgcggca
     2221 accgcacgct cgtctcgcac accgccgccc agcatttcgc cacattgtaa gcgggaaagc
     2281 tcacgagatc tcaggggaaa aatgcgctcg acccctgcgg cgccggggtg tagaacgacg
     2341 ccccttatga agtccctagc ttgtgtttcg atgctcgttg cggcggccct gacggggtgt
     2401 ctcgccgacg gcggcggggt cgatggcgac cagacggagc aggagtcgac cacgacgtac
     2461 gtcgacatcc tcgacttctc gcagaccgat cagagccagt ggtacgacct catccacaac
     2521 ctcaaccacc agttcgacgc cgtctgtggc gacacgttct gcgagggtga gtactcgaac
     2581 atccagccgc tcacgttctc gtgctcggtg tcgagcaagg ccggcagcgt gaaggattgc
     2641 gcgtggacgt ttgccgcggc gcaggtcgat gtcgatccgc gcaccgcagc gatcgtcgtc
     2701 gacgcgccta cgttccagtg ccacttccag atgaagacca cggcgaagcg cctcgttggc
     2761 attctcgccg gcgcggaccc gctgcacgcg gcactgcctg gtgcacccgc ggcgacgccc
     2821 tcgatttatg accagctgag cgcgtgcttt cagcatccga tcggccagac gcccggcacg
     2881 tttgccgaga ccggcagcct cagctatgtg tcggcgcgcg attactacac gtcgtcggcg
     2941 ggcatcgagc gatgggccgc tggcaaggca gcgctcaagg acggcttcga tcgcgtgtgc
     3001 ggcgacacgt tctgcagcgg cgatttcggc gacctgcagg cgatggactt cacctgcgcg
     3061 atcacgaagt cgaccggcaa cgtgaagtcg tgcatgtgga cgtttggcgg cagctggcac
     3121 ctcgtcgcag agcacggcgg cgcgcagcag atcacgcagt ccacgttcaa gtgccccgtc
     3181 ctcatgaagg gcacgctgtc gaagttcctc gacgtgctgc tcgcgcccgg caccgaccag
     3241 ccgatcaacc gcccgcttcc cggcatgacg acgtcggcgt acgaagcgct gctcggttgt
     3301 ctgccctgat cgcgctcgcc gagacgcggt tcgggcgcta gaaacgagtc gtggaagaag
     3361 agccgaacag gctccgggcc tcgattcgtc tcgtcgtccg gcgctatctg cgtcaggtgc
     3421 gagcgtggcg gtggctgtcg gcggcgtcgc tgctgttgcc aggcatcggc aacatcttcg
     3481 tccactacgt gccgccgctc gcgatcgcgc atctgctcgc gacgctcgcg cgcgacaagc
     3541 atccgtcgac gggtgagctc gcgacgccgg tcattctgct cgccgttgcg tggttcggcg
     3601 gcgaagcgat ctggcgattc gcgagcttct tgctgtcgcg gctcgagtac cacgcgatgc
     3661 gcgcgctcta caacgaggcg atggacgagc tgttcgtgaa agacgttggc ttctttcaca
     3721 acaacttcgc cggctcgctc acgaagcgtg tgctcggcta cgcgcggcga ttcgaggacg
     3781 tgttcgacgt gttcgcgttc tcgatcgtcg gcaatctgtt tccgctgacg ttcgcgatca
     3841 tcgtgctcgg aaaattctcg ggctggctga tcctcgtctt gctcgtgatg ctgacgttcg
     3901 cgttcttctg catgcggccg ctgatccgca aacgccacgc gctcgtgcag atccgcgaac
     3961 aagcgagcaa cgtgttggcc ggtcacgtcg cggactcgat cacgaatgcg gagaccgtgc
     4021 gggcattcgc gcgcgagccc gaagaggcga gcattcacgc gaagaacgtc gacgacttca
     4081 ccgcgaagat gctgcgtgcg tgggactacc agaacacgcg tgtcgatatc gtcacgtcgc
     4141 cgatctacgt attcacgaac atcgccggtc tcgtcgtcgc gctcgtgatc ggccgcaaca
     4201 gcgggttcga gatcgaggcg gtatttttga cgttcagcta cttcgcgcaa tcgacccgcg
     4261 tgatgtggga gttcaatcgc atctatcgca acctcgagac gtcactcacc gatgccgcgc
     4321 agttcaccga gctcctcctc gagccgccag cgatccagga tgccgagcgc gtcgaggcgt
     4381 tcgagccgca agactcgagc gttcggtttc gctcgatccg cttcgcgtat cctgggcggc
     4441 caccgctgtt cgacaacttc gatctgacga tcgcgccggg cgagaaagtc ggcctcgtcg
     4501 ggcgctccgg cggcgggaag accacgatca cgcggctcct gctgcgattt gccgacgtgc
     4561 aggcgggtgc gatcgagata ggtggccaga acatcgcgct catcgcgcag tcggcgcttc
     4621 gcgatgcgat cgcggtcgtc ccccaagagc cggcgatgtt ccaccgcacg atcgcggaca
     4681 acattcgctt tggccgtccg aacgcgaccg acgacgaagt gcgccgcgcc gccgagctcg
     4741 cgcatgccga cgagttcatc gctgcactgc cggccggctt cgagacgctc gtcggtgagc
     4801 gcggcatcaa gctgtccggc ggccagcgcc agcgcatcgc gatcgcacgc gcgatcctca
     4861 aggacgctcc catcctcgtg ctcgacgaag ccactagcgc gctcgactcc gagagcgaga
     4921 gccacatcca agatgcgctg cgtacgctga tgaaagaccg cacggcgatc gtgatcgcgc
     4981 atcgcctctc gacggtgcgg cggatggacc gcctcgtcgt gctggaagag ggccgggtca
     5041 tcgagcaagg ccggcacgac cagctgctcg cgatggccgg ccaatacgcc gcgctctggg
     5101 agcaccaatc cggcggcttc atgcaggagc gcgccgtatg atttcgcgga gctagcgatt
     5161 tgttcgaggc cattgtcgat cggggcgccc cccatccgtc gcgtccttga aaggtagaga
     5221 agcgatgccc acccaactat tgaacgatga cggcacggcc tcgatggcga cgatgctgat
     5281 gtcctcgcac cacgccttcc gccgcgacgc cgcatgcttc gcgctcgccc tcgccgatgg
     5341 ctcgcgtccc gctgccgtgc tcgccgagga gtgggcgcgc tttcgcggcg cgctgcacgg
     5401 tcaccacacg atcgaggaca cggcgatgtt ccccgacctg cgcgcgaagc accccgagct
     5461 cggcgcgacg ctcgacaagc tcgatgcgca tcaccgcgcg atcgatccgc tgctcgagcg
     5521 cggtgacaag ctgttcgcgg acctcggcac gcagcgcggc gccgccagcg atctgatcgc
     5581 gacgctgcaa ggcctgctga aggagcacct cgatctcgag gagcatgcga tcacgccgca
     5641 cctgcgcgct gccaaagaat tcccgctgcc gccgaccgaa gacgccgctg cgatgtacgc
     5701 cgaaggtttc gcgtggagca tggcgggcat ctcggacgcg gtgctcggta agatgcgcga
     5761 gatgctaccc gccgcgctcg tcgagaagct cccagcggcc cgcaccacat tcgacgaacg
     5821 gtgccgccgc gtgtggggct acgcgcacgc aggtgcgtcg acgacgtcgg atccggcggc
     5881 tacagcgcgc acgtaaacag cggcgtcggt gcgttcaccg cggagagcgt gaactcgaag
     5941 ctcgtcgcgg tgacgtcgaa gccgtccgtt ggcgcgcgtg ccggcgcctg gcacgtgaag
     6001 agaaggtcga agctcgacgt gtagctcgtg ccgctcgagc acgcgagctc gaactcgcga
     6061 tcgggatgaa gctgcgagac ttcggtcttg ccgtcgacgg tcagctccat gcggttctgg
     6121 aacgtgtcga cgaagcgcca cgcgcaaccg cccgggcccg tgacgtcgaa ggcgaggcgc
     6181 tcgaggtcga tcgtgtcgtt cggtagcggc gtgtcgaacg tgacctgcgc gccgtcgggc
     6241 caggtgcaat gcttgcgatc ggccgagagc gtcgcgggcg tcgcacgaga cggcaagcat
     6301 gcggcagcgc gctcggcagc cgacgcccag cagtttgtcg ggtcggcgag gagctcgcag
     6361 gtgaggtcct cgctgctgcc gcaagcggcc gtagctagga caatcgcgat ggggagatgc
     6421 cgcacgcaga ccagtgtact tcgggcttgc aatattcaac caaatggtga actatctgtt
     6481 cgtggccagc ctcgatacga cgttctcggc gatttcggac ccgacgcgtc gggcgattct
     6541 cgaccggctg tcgcgtggcc ctgcgcgcgt gaccgatgtt gccgagccct tcgcgatgtc
     6601 gctcgcagcg gtgtccaagc acatcaaggt gctcgaggat gccgggctcg tgcggcgcgc
     6661 gaagaatggt cgcgagcaca cgctcgagct cgaggcggcg ccgctgcgag aagccgcgca
     6721 gtggacatcg ggctaccagc agttctggac agagcgcctc gatcggctcg aggccttctt
     6781 caagcaaagg aagcaatcat gaagctcacc gatctcgaag ttacccgtca gattccagcg
     6841 acgcctgaag aggtctacga cgtctggatc gatccgacct cgcccggtgg tccgtggttc
     6901 agcccgcagg ccgacggcat gtcgtcgaag atcttgttca ccgccgcggt cgacgggctc
     6961 ttctatcact gcgtcacgaa caagggtcag gcctgggcgc actacggccg gttcatcaag
     7021 ctcgagcgcg gcaagctggc cgagcacacc tgggtcggcg agtggacgaa gggccgcgag
     7081 agtgtcgtga cgaccacctt ccaagccaag gacggcggga ctctcgtgac gctacggcac
     7141 accggagtgc ccgacgacga ggagggacgt cgtcatgcac agggctgggc gtggtgcctg
     7201 agcatgctcg cggacaagat gggttcgaag aagtaatcag ccgtacgccg cggctagagg
     7261 ccgagccatc cccggcggcg cgctgtgtcg acggagcacg cggagcgccg ttatccgacc
     7321 cgggcccacc tgattggtgt cgcgaggttg cacatcgcgg ctcaccgcgc actccgcaaa
     7381 cccagggcac agcggatgca cctcgtgcgt cgccatgaac cgcgcggagc tcgagaccac
     7441 tgccccgatc aaacgcgaag agctgctcgg cctgctcaac gcgatgacgc cggccgagca
     7501 gcaggcgatc accgcgcgcg cgtcgatttc ggccatcgaa gcggagctcg cgcggcaacg
     7561 cgaagcgtcg cagccctcgc agccctcgca ggccgaggcc gaggccgagg cggtggccga
     7621 ggcgccgccg cgccggcgct cgcgcaaggc caccaaagcc gagaagcgcg ccgcgcgaaa
     7681 agcggcgagg aaggccgcgc gggccgcgtc gcgggcgcaa acccagcgcg cgggcgacgc
     7741 cgagtcgacc aacgaatcga tcgacgaatc gatcgacgaa gccatcgacg aatcgaccga
     7801 cgcatcgaac gacgaatcga tcgcggccat cgcgatggcc acaccggcga atacgaacgt
     7861 agcgacgacc gcggcggcaa acacgaacat cgcgccgacc gcgacgagcg ggcaagcaac
     7921 cgacgcgacc ggcgacatcg cgatgagcga cgcggcgact ggcgcggcgg cggacagcgc
     7981 agccggcgac acgagtggca cgtctggcgc ggcggcggac agcgcagccg gcgacacgag
     8041 cggcacgtcc ggcgcggcgg ccgacagcgc agccggcgac acgagtagca cgtctggcgc
     8101 ggcggccgac agcgcagccg gcgacaacaa gctgttcgag gatatcgact gggacaacct
     8161 gctgacgaac gcggtgtcgt cgatcaccga gccgaagccg tcgcgcgcca cgccaccgcc
     8221 gattccggcc ccggcgagcg accggcccaa gcgggcgagg acggtgcgtc cgttcgcgca
     8281 acgcgcggcg atgctgccga tctgtgagcc gaagcgattt cctcgcgcaa cgcagacgcc
     8341 gccgtacgtt cgtgcggctg ctcacaacac gtcggtcgcg gcggaggaga aagcagcacg
     8401 ggtcgtggcg gaagttcgcg cgcgcgtcgg cagcgagcac gacatggccg aaggcagcgg
     8461 tccgtgcgac gcgatcgatc gcatcagcga cggcgttcct tgccccgtgc tgtcgcggcg
     8521 gatcgtcgcg agcctcagcg gcgaagagat cgcgatgctg ccgatggagc ttcggctcgc
     8581 actgccgaag actccgacgt gggagaaccc gccgcgcatc cagatgacgc cggtgcccga
     8641 cggcgaatgc atcacgatcc actcgaccaa gccgaaggac agctgtgcgg cgatgtctgc
     8701 gggcgcgccg gaaatcgcca gcgtgccgag catggctacg atcgttgcga gcttcgcagc
     8761 aacagtcctc gtcggactcg cagcgttcta cttcatcttc tgagcgtaca gaacgtaagg
     8821 gagcgcaaga atctctcaga cgggagccgg agcgcggtgc ggtagcatca gccgttgctt
     8881 caacctggcg cgatcgtcgc gggacggttc gaaatcgaag agctcgccgg ccgcggtggg
     8941 atgggcgcgg tctatcgcgc gcgcgatcgc cagaccggat ccgttgtcgc gctcaaggtc
     9001 gtgcacgaag aagcgcctcg cgatcatcac gagcgcctcg tgcgcgaagc gcggttgctc
     9061 gctgagctcc agcatccggc gatcgtgaaa tatctcgcat tcggcgcgac cgatcgcggc
     9121 tggccgtatc tcgcgatgga gtggctcgaa ggtgaggact tggcgctgca cttgcgcggc
     9181 gagggcctcg atccgagcga tgccgtgacg atcgcgctgc gcgttgccga ggcgctgggc
     9241 atcgcgcacg agcgaggcat cgttcatcgc gatatcaagc cgagcaacct gtttctcgtc
     9301 ggtggcgatc cggcgcgcgt gaaggtgctc gacttcggcg tcgcacgcat cgagctcgct
     9361 gatgacggcg tgacgcgcac gggtgcggcc gtcggcacgc cgcgatacat gtcccccgag
     9421 caggcgcgcg ggagccgcga cgtcgacgtc cgcgcggacg tgttcgcgct cggctgcgtg
     9481 ttgttcgagt gcctcgcagg gcggcctgca ttctcgggtg caaaccaggg cgccgtgctc
     9541 gcgaagatct tgctcgaaga agcgccgcgc ctcacgagcg tgcgcgacga tcttccgccc
     9601 gcactcgacg agctgctggc tcggatgctc gcgaaacagc gcgacgatcg tccccgtgat
     9661 gggcgagcgg tcgcggccga gctcgctgcg attggcgacg tcggcaccac agcgcggcgc
     9721 acggcaggac ctcgcgatgc cggcgtcacg accggcgagc aacgactcgt gtgtgtcgtg
     9781 tttgcggaga ccgcgctcga ggtgtcggcg ggattcgagg agcggacgcc gagcacaccg
     9841 tcgacgagct ttgcgtggcc ggtgaagccg gtggccggct ccgacgaaga gacagcgacg
     9901 ctcgatccgc ggcggtctgt tcgcccgacg catcaggtcg tgcgccggct ggagacgatc
     9961 gtcgccgagc atcagggccg cgccgaagtg atgaaggacg gctcggtcgt cgtcacgctc
    10021 tcggggcgca gcgcggcgac ggatcaagcg gcgagtgccg cgcgctgcgc gctcgcgttg
    10081 cgcaagatct tcccgaacac ggcgatggcg ctcgcgatcg gccgcagtgc agtgagcgat
    10141 cgctatccac tcggtgacct gatcgatcgc gcgaccgccg tcctctcgcg gccaccacct
    10201 gaagcggtcc gccacgggac gttcccgatc tacgtcgacg acgtcagcgc cgcgctcctc
    10261 gacctgcgct tctcgatcga aggcgatgcg aatggcctct atctgtcgcg cgagcgcgat
    10321 gtcgcggtcg ccgcgcgcac gctgctcggc aagccgacgg agtgtgtcgg ccgcgagcgg
    10381 gagctcggca tgttgctcgc gacctttgaa gagtgcgtcg ccgagccgat ggcgcatgtc
    10441 gtgctcgtga cggcagcggc gggcatcggg aagtcgcgcg ttcgcttcga gctgatgcag
    10501 cggctcgccc agacggggcg gccgttcgag ctcctcctcg gtcgaggcga tccgatgagc
    10561 gccggtacgc cgttcgcgat gctcgcgcag gcgctgcgtc gtgcggccgg tgtcgtcgac
    10621 gacgagccgg caacggttcg ctatcgcaag ctgcgcgctc gcgtgttgcg caacgttcca
    10681 ccgcgcgatg ccgagcacgt gacggagttt ctcgccgagc tcctcggtgc tggaccgccg
    10741 ggcgagggca gcgtgcagtt gcgcgccgcg cgtcaggacc cgcagctcat gggcgatcag
    10801 atgctgcgcg cctgccagca gttctttgcc gccgagtgcg acgcgaatcc ggtcgtgctc
    10861 gtgctcgaag atctgcagtg gagtgatcgc acgacagtgc agttcgtcga cgcgatcgtg
    10921 cgccagctcg cggagaagcc gctgttcgtg ctcgcgctcg cgcggcccga agtcgatgcg
    10981 gtgttcccgc ggctgtgggc ggaccgtcgc gtcgagcgcc tgcgcctgca cgagctgacg
    11041 agacgcgcgt cggagaagct ggtgcgcgag acgctcggcg atgtgaaccc cgcgacggtc
    11101 gcgcgcatca tcgatcgggc ggcgggcaac gcgttctatc tcgaagagct gattcgtacc
    11161 gtcgcggagg ggcacgagga tcgcctgccc gagacggtcg tcgcgatggt gcaggcgcgc
    11221 atcgaatcgc tcgaggtcga ggcacgccac gtgctgcgcg cggcgagcat tttcggccag
    11281 gtgttctggc gcggtggcgt cgaggcgctg ctcgaaggtg gcacgacgca agtcgacgat
    11341 tggctcgacg agctagcgcg acgtgagctc gtgaccgttc gccgcgagag ccggttcagc
    11401 ggcgaggcgg agtacacgtt ccggcacgcg atcgtgcgcg aagcggcata cgcgatggtc
    11461 accgagcgcg atcgcatggt cggccatcgg ctggccggcg cgtggctcga agcgcgcgtt
    11521 gcgaccgaag gcgccgacat cgaagcggtc gcgctcgccg agcacttcct ccgaggcggc
    11581 gagcccgcgc gatccgtgac ctggtatcgc cacgcggcgc agcaagcgct cgaaggcgat
    11641 gatctcagcg gtgcgatcga tcgtgcggag cgcgcgatca cgtgcatgga cgacgcggat
    11701 ccgaccgttc gcgatcgagc gctcgtcggc acgctgcgct cgctgcaggc cggtgcacac
    11761 gtgtggcgcg gcgagtacgc gattgcagcg gagcgaggca acgaggctct cgcggtgctg
    11821 tcgccggcga gcgtcgcgtg gctctcggca gcggccgcgg ttgcggatgc atgctcgcgt
    11881 cgcctcgaac atccgtgggt gctcgagcta tgccgccggc tcgccgcgct cgaagtaacg
    11941 ccgaacctct atcacgcgta cgcgcatgcc gtcgcgacga cgatgacgac gctgctctgg
    12001 cacggcgacg ccgatctgag tggccggttg ttcgcgcagc tcgcgcgcat cgaagcggac
    12061 cgccccggtc ccgacacgct cgcgtggatc tacacggcac aggcgtggcg cgcgttgcac
    12121 gcgggcgatc atgccggagc gctcgctctc gatcagaagg tggagcgctg cttcaccgag
    12181 gtcggcgact tgcgccaagc gtgccggcag caggcgagcg tcgggtacgg ccagctcatg
    12241 ctcggcgcga atgtcgaggc ggagcaatct ctgcggcaat cgatcgcgat cgcaacgcgc
    12301 atcggcctgc atcaagtgac gacgcaagcg aagcacaacc tcggcatctg tctcgcgcgc
    12361 atcggcaagc tcgaagaggc tcgcgacgtc gagcgcgacg cgctggcatc cttcgaggcg
    12421 caggagaacc gccggctttg cgcgttcgcg cgcttctacc tcgccgagat cgagctcacc
    12481 gcgggtaacg cagcgggggc gctcgcatac gcgcgcgaag cactcgtcgt cgatgccgag
    12541 cagccgagct tccactgcgt atatcgcgcg cgaatcgcaa atgcgcatct cctcgccggc
    12601 gattccgcgg ctgctctcga cgaagcgaag caagcgatgg agctcatgga acgccacggc
    12661 cgccccgaag aaggcgagat ggtcgtccgc ctcgcgtacg cgcgcgcgct tcacgcaacc
    12721 ggcgacgctg cgaccgcgcg cgaggtcatc acggatatcg aatggcgtgt gaccaccgag
    12781 gccgcgcgca tcggcgatcc agcattgcgt cggtcatttc tggaaggcgt acccgagcac
    12841 gcggtcgcgc tgtcgctcgc gcgcgagtgg acctagagcg tcctgtagat cgtgagatcg
    12901 gcgtccacct gcggcaccgg ctcgagccac ggccgttccg gaagcgacat gtcgaggcaa
    12961 cagatgcgga gcggctgtgc tcgcgcgatc ggctcgaggt tgtcgagaaa gcgcgagagc
    13021 cgtgcgccgc tgaacggaca atagaggaag tacacggtgc ccagcggcag ctcggtaccc
    13081 gcgcgcgacg catctgcttc gatcgtcgac atcgcatcac gcagcccgcg cgacgctgca
    13141 acgagcgccg gttgaatctc gatgccgata gcgcgcgcac cagtcgcgag atgaacgaac
    13201 gcagccgcgc gaccgacgcc cgagccgaca tcgacgaaca cgtcggacgg tcccaccgcc
    13261 gcacgttcga tcacgcgcag cagccgatcg acagagcagg gcaggtacgg cacgcaatct
    13321 cggggcagat ccgcgccgtc atcgggcaga tcgccaagac ccaaccgcgc gtcgagccac
    13381 gcgtcccggt cggccggagc gaccgccgcg agctccgcgc gaaactgcat cgcgtgagca
    13441 tagtgcaggc ctgccgagca cataggagat ttcgtgcgac catggccgtc ccaatggctc
    13501 actcatccat gaagcagact atcggcagca tccttctcgc agtggctctc tgtgcatgtg
    13561 gcaagggcgg cggcggcgac atcgacacat tcatgaagct cgacagcgac aaggccgctg
    13621 cgttcgccgc tggcggcgac gactgcgaag ccaaggcgaa gagcgtcggc gattggcgca
    13681 aggcgcacac cgccgagtac aaggcgcttc agaagaagct caacgatcag tggtcgagcg
    13741 gcccgccgaa ggacgtgcag gacaagtacg gcgacaagat gaaggcgaac aagaaggccg
    13801 tcatcgacgc gatgttcaag tgcaccgaca acgaagcgtt cggcaagatg atggacgaga
    13861 cgaagaccga ctagcggcct agagaatgat cgggttgcag tggctgtcga ggacgacctc
    13921 gttgatggtc gccgacagcg acagcgtgcc cgctttgatc ttgtccagat cgtagtttcc
    13981 gccgtccgtc tgcgtcggga tcggcggctg tgacttgtag ggtgtcgacc aaccgtccgt
    14041 gatcgacgcc cacggcagcc gccgtcccgt gccgcagcga tcgaggaagc tcggctgcgt
    14101 gatgcgcgag aacgagaaca cgcggatctt gttcatgcgc agcgacgcga ccgtgttcgc
    14161 catcgtgcgc gccgccggat agttaccctc gcgcgggaag tcggtcttgt tcgtctggcc
    14221 gtcgccgtcg cggtcgccgt agttgtcgct cgcctcgagg aacgtgtcgt cggtcgcgag
    14281 gatcacgatg cgtgtcgcgt tgctacgcca cgggaattca ttcgcggctg catacagcga
    14341 gtcgagcgag ttctcctcgc agatccgatt ctgagtgggc tggccgctcg ggccgtcgcc
    14401 ggggttgcgg ttgtacgcgg tgtagttgtc gcggtagttg cgaaacgcgg tctgcagcga
    14461 cgtcgcgctc gtgtggatgc tcatcatcga gccggtcttg tcgagcgcgt agttgtcttg
    14521 gaacgcgatg agaccgaagt gcgagtcagc cgcaaccatg ttgctcgccg tgacgacgtc
    14581 ggcgatctgc gcgtcgagct tcgtcaacac gaagtccatc gagctcgacg tatcgagcac
    14641 gaagacgacg tcgatgactt ctttgcactc gcctgcatcg ggctgcggcg tgttcgagcc
    14701 cgcagcatcg gcagtctcgc catcgcgcga cgtcggaccg catgcactcg caacgacgac
    14761 aacgaataca gcggctctca tcgcgcgcga gtatgcatgc cgcccccggc cagcgggatg
    14821 acagctcctc accggggtga ggaccgctac tgctgcttaa tggttgcgct taccgaccgc
    14881 gacgctggcg gggcgcagcg tctcttcgcc gacgagatag ccctcgcgca tcacatcgat
    14941 gacgcggcca tcctgcgcgg gatcggtgac cggaacgagc gcgaccgcat cgtgacgctt
    15001 cggatcgaac ggctcgccgc gcgcgggcgc atgaaccacg ccgaactggc cgagccgtgt
    15061 gagcatgttg cgccgcacga gctcgatgcc ctcgacgagg tccttcgccg ccgttccctt
    15121 cgcagccgcg atcgcgcgat cgaggtcatc gaccacctcg aggaagctct cgagcatctt
    15181 gcggctcttc tgctcgagct ccttcgccga cgcattcgcc atgcgcttgc cggccgcttc
    15241 gatttcggcg tgcgcttgat ccgctcgctg gtttgccgcc cgcagctcgg catctttctt
    15301 cgcgatcgcc gcgtttagcg cctcgacatc gtcctcgaga gacgcgacgt agtcggcttc
    15361 ggcggtggtg tcggtgtcgg tgccgccgag taagtcgtcg agctccttgg ctgctgcaac
    15421 tgcatcaaaa tcaggcgagt cagaatcggc cacgcgctga gcctagcagg tagtaagctc
    15481 gtcgcatgtc gttactcgac cgtctcgtct cgtacgccga ataccatcgc gacaagcgca
    15541 acatcgcgac gcacttcgtc ggcattccga tgatcgtcat cggtacgcag tccgcgctcg
    15601 cacgcatcgg catcgggccg atcaacgtag ctgccgcggt cactgggctc gcgacccgct
    15661 attaccgcgc gatcgatccg agctacggcg cggcgatggg cgcgatcctc acggccacgt
    15721 atggcatcgg cacgatgctc gcggcgatgc cgctaccgat ctgggcgggt acgaccgccg
    15781 gcctgttctt cggcggctgg gcgctgcagt tcatcggcca tttcttcgag ggcaagaagc
    15841 ccgcgttcat ggacgatctc aaaggcctgc tcgacggccc gctgttcctc gtcgcggaga
    15901 ccgcgttcgc gctcggcttc tcgaaggagc tccgtgccga agtcgagcgt cgcgcgggtc
    15961 cgacgcgttg gggcaaagtc gccgaagcga ctgcgcctgc gttcgcgcac gccgctgcag
    16021 cgaactaatt ctgcggaccg acgtgcgccg gcgtgcccgc gagggtgtcg ccgatccact
    16081 tcgtcagcat cacgttgccg gcctgcgtcg tgtagaagtt tgccgagcgc aggatcgtgt
    16141 ggctgctgcc agagatgtag tacgtgccga agttgccgcc ttcgccttgg accttcgtgc
    16201 ggagcgcgtt gaggcccgcg gtgtagacgg tcgtcgtcgc agcgctcggc gtgtccttgc
    16261 acatgttgta gccgccggac cagccgtagc ccgcgaacgt gcgaatcgtc tggtcgcccg
    16321 tgctcgagaa caggccgccc ttcatgttcg gaaattcctg gcgcatgtga tcgaacgtct
    16381 cttcgacgaa cgtcgcgccg ctgcagtccg cgccgcactc tttgacgacg gtcttgtcga
    16441 ggccccacgt cgtcttccac agcgtctgca ggcatggcgg atagacgtcg gccgacatcg
    16501 gcggacccga gtcgtcgatc agcgtgaccg ggatcgatcc gtatgcgcgc tgcgtctgcg
    16561 tgtagttcac cgcggcgccg aaaccgcctg cgctcgcgcc agtgagcagc gcctgatcga
    16621 cagtgaagct cggcacgatg cgctcgagga acgccgtcat gttcgagtag ccgtagaagc
    16681 tgcgcatctt gccgccgacc atcgtgtcgg cttgcccgga gtagacgtcg cccgagcaat
    16741 acggcacgta gacccacgtc cagtccttca gcggattggc cgcgttcgcg cgatcgaaga
    16801 tgccgcccgc cgacggctga ttaccgccgc gcgagaacaa gctgtcgcag aacgtgttga
    16861 agcacgcgcc gccgccctcg aggtagatcg cgatcttcgt cgacgcgggg ttgtagttga
    16921 cggagaaccc ggccggcgtg ccgtcgatgc acttggtgcc ggggatttcg aagtagcgcc
    16981 acgccatcgg gtcggccgtg agcttgtcga ccatcgtgca gtcagagcgg caaccgtcga
    17041 agttgttgtc gttgccgtcg tcgcattgct cgccggtgtc gacggagttg ttgccacacg
    17101 tcgcggggcc gtcgtcgccg gtcatgtcgt caccgggcga tgacgtgcaa gcggccaaga
    17161 ggagtagaga acagaacagg ctacgcatcg tgtgcctccg cggcgtacac tcgcgccgag
    17221 tcgttttgcc gtcttgaccg aatagaccgg agattgacct cgagacctgc tctgtatgga
    17281 tggtgtgctc acctacagta cgagcttctg ccggatcctc accgactatt tggtgtcgtg
    17341 cggggcgtcc gtcgagccgg tgatcgcggc gatgggtgtc gacgcagcag cgctcgaaga
    17401 tcgcgacggt cggatcccgc ggcgtgcgtg gctgcatgcc ttcgaggtcg cggtcgacgt
    17461 cacgggtgat cccgatctcg ggctgcgcgt cggagagagc atccgcccgg cgcatctcgg
    17521 tgcgctcggc tacatcttga tgagctgcga gacgatgaaa cagaccgcac aggtcgagtc
    17581 gcgttggcat tccctcgtcg ccgacggaga gcggctcgaa tatgtgcgcg aaggacagct
    17641 aagccggcgt acgttgatct tgcccgaggg tgagccgcca ccaccggcct gcgcagctgc
    17701 atgtgccgcg gccgcgagcg tgtccttcgt gcgttggctc gcgggtgtcg gcggcgatga
    17761 cgggctgcgc cgcgtcgaac tgccgtatcg ggagccatca tcgcgcgcgg gtcacgaccg
    17821 cttctttcgc tgcgagctcg tgttcgatgc gccgcacgtc acggtctggc gagatccggc
    17881 cgcgctgcgc aagccactcg cgcaagcgga tccatcgctg cgtgcgcgca tggaggagcg
    17941 agcgacaaag ctagctgcgg agcggagcgc gagtgaccag ctcgtgacgc gcgtgcgcga
    18001 gctcgttgtg cgagatattc gcgaaacggt gccggacctc gaagccgtcg cggcggcgct
    18061 cgagcttcca cctcgcgtgc tcaagaaacg gctcgccgac aaggccacga gctacacgaa
    18121 gatcgtcgac gacacacggc gtcaattggc gctcggctac atcgcggatc cgacgcttac
    18181 gctcgtcgat gtcgcgtact tgtgcgggtt ctccgaacag agcgccttca atcgcgcatt
    18241 caagcgatgg accgggctgc cgccgggcaa ctaccggcgg gggtaggcgg accccggcga
    18301 actggcgaat ttgtgacacg aattgctcgc gttggaagcc accttagatg atggtcccct
    18361 ccatggcgtc ccaagcagag cttcgtgagc tgagaggaga attacgcgat gccggactgt
    18421 tcgagcattg cgagctccgc agctggctga agctcggagt gctcgtcgga ggcgtcgctg
    18481 cctccctcgt cacgatggcg acattcggcg catggaccgc actgttcgcg attccgatcg
    18541 cagcggtctt cgcgacggcg gcggcgatgg tcgcgcacga aggtagccac cgcagcttct
    18601 cggcgtcccc cacgcggaac gcgttgatgg tctacatggt gttcccgctg ttcacgggcc
    18661 ttggctcgct gtactggcgg cacaagcacg atcgcttgca ccacggtcac cccaacgtcg
    18721 agggcgtcga cccggacatc aagccgttcc cgttcgtgtc gtcacgcggt gaccacgaga
    18781 agtgcgggcc caagcaacgc tggttcgtga gcaagctgca gcgctgggca ttctggccga
    18841 tgtccacgct ccttgccgtc ggcatgcgcc gctcgtcgtt cctctatctc atccgctatc
    18901 cgcgcgagca cggcatcaag gccgcgtggt gcgtcgaggc cctctgcatg accgggcact
    18961 acgtactctg gtacgtcatc ccttgcttca tctggggacc ggtcgcgacg ctcctcgtga
    19021 acgcgggcat ctgggggctc gtcggcgtga tgctcacgct cgtgttcgcg ccggcgcata
    19081 tcgggctgcc ggttctcgtc gagccgcacc acgattggca gcatcagctc gagacgactc
    19141 gcaacctcca gctgcctcgc gtgatctcgt tcttcttcat cgggctcgat tatcaggtcg
    19201 agcaccacct gttcccgaag atcccgcatc agaagctgcc ggctgccgcc gcgatcacag
    19261 cgcgctggtg caagaagcac ggcgtgccgt accagagcgt gccgtacatg accgcgctga
    19321 aggactcggc gcagttcatg gccgatgcgt ggacgcgcga cgccgagtac gcgtcggtgg
    19381 ttcgcgcccg tatgctcgac gctgtcggct agctagcgcg gcagtcgggc tgcttgcggc
    19441 cggtcttcgt caagaccatc tggtagagct gaccatcgcg agagcgcgac gcgccggctg
    19501 ctgccaggag atagaacttc cacatctggt agaagcggcg atcgtagcgg tggctgatct
    19561 ccgagtacgt gcgatcgaag ttctcccacc acgccatcag cgtcgggtcg taatccgggc
    19621 cgatgttgtg gaggtcttcc atcacgaaga cgccctcgat cgcgcgaccg atctgcgcga
    19681 gtgacggcgc gaccgcgttc gggaagatgt acttctcgat aaacggcgta ccgtggcgca
    19741 ggctgcggtt gttcgcgatc gtatggacga gagcgacgcc gtcgtcgacg aggcagcggt
    19801 cgatcgtctc catcatcatg cggtgattct tcgggccgac gtgctccatc atgccgatcg
    19861 acacgacgcg gtcgaacttg ccctgaacgt cgcggtagtc gcagaggcgc agctcgacgt
    19921 cgagcttctt gccgttcggt ccccacagct ggttaccgag cgagacctgg tcgtgcgaga
    19981 gcgtgacgcc gaggaccgag cagccgtact tctcggccgc ccagctcgcg agaccgcccc
    20041 agccgcagcc gagatcgagc acgcgcatgc cgggcttcat gccgaccttg cgacagacga
    20101 gatcgagctt cgcttcctgc gcctcggtca gcgtcttggc gtccttccag tagccgcacg
    20161 tgtagaccat gcgctcgtcg agcatgcggg tgtagagatc gttgccgatg tcgtagtgcg
    20221 cctcgacgtt cgcaccggag cgcgacttcg cctggaggtt gagcacgacg gccttcaccg
    20281 tgagcgcacg catgcgccag ctgccggtga tcttctgctt gaggttcgcg cgcatgatct
    20341 tgtcgatcgt gacgtcgagc gcgtcggtct cccaccagtc gtccatgtac gcttcgccga
    20401 agccgacaga ggcatcgcgc aggacgcgct cgtagaagcg ctggtcgctg acgcggatgt
    20461 cgccgacgtt cgtgccgccg atctcgatgc cggcgtgggc aaagagctcg cggatgatcg
    20521 cttcggcgtc agccgagcgt gtcgcggtca gcgtcttgag gggccagagc tggctgagca
    20581 gctcgcgggg agttgttctc tggggagctt gtgcgtgctg catcgcagcg gtcgtcgcac
    20641 gtgccccggc gaggcacaag cgaaaccggc acgaaccgag gctcagacct cggtgtcggg
    20701 ctcggacagc tcttgccagc ttcgacgggc ggctacgtac gcgaggcgcg ccagctcttc
    20761 gatcacgtcg agctcgagct tgaggcgaaa cgcgccgcgg atccgcatgc gagcctcgcc
    20821 gagcgtgccc ggccacgagc cgatgatctg tcggtcgtcc gcgcgaagcg aacgcacgag
    20881 ctcgctggcc cacgcttcgc cgacggcgat cgcgcgcgga tgcatctctg gttgcacgat
    20941 ctcagtcacc ggcgccccct cgcgatttcg gtccgatgag cgcagcgcgc acgcggcgtg
    21001 cgagatcatc atcggggttg agcgacgaca gccagtgggc aagcccgtcg ggctggagcg
    21061 gctcgtcgcc gatcgtgatg agcctggcgc tcgggtgata ggcagcgacg aacttccgca
    21121 tgtcctcggc tgcacctgtc gggcgtgaat cggccaccgc gacgacgtcg ggctcgaagc
    21181 tcgactcgcc gaggcgcacg atcgcttcga gcggctgcga cgcctcgatg acgtggcatc
    21241 ccgccgagcg aaagccgtcg gccatcgcgt tgcgccgcgg ctggtccgca tcgacgagca
    21301 cgacagacag gatgcgagca cgagcgtgcg ctgatgacga gatatccaga atgactttgc
    21361 gcagcgtcgg agggatgtcg tcgaacgcga tcgcgatgcc gttcggccgc aggcgcgaga
    21421 cgcgtcccgc agcgcgagtc cactcggcgt ggccggcgtc gagcctcagc tcgaattcga
    21481 tcgtgcgtgc gagcatccga tcgggcaagg cgaccttcgt caccacgtac acaccaccga
    21541 ggctgatgtt cgcgatccgc ccgcgctggt tctgatcctg accgtgaaca atgagcgtcc
    21601 ccttcaccgc gattctctcg tggcgacgtc gctccgcttg ccgttcggtc atcgaccggg
    21661 cctagcgttt tccgctggac gagcccgagg gagtcgaaga gggattcgaa gaattgctcg
    21721 aagaattgtt cgaagcgctc gtggggctgg acgggttcga cggggtcgaa gacgaattgg
    21781 ccggcgtcac gggagccggg tcatcagcgg tcgacgcgtt ctcggtgcca cctggttggt
    21841 cccacgcgat ctgcgggccc ttggtgccct cgctgttcgg ggcgcgattg ttgcggcgct
    21901 cggccttctc tttcgcgcga tccttcaact tcatctcgcg attgcgtttt gcgaaagtgc
    21961 ctcgttgatt tgccatcgtc actcctaagg ccaagtaatt cagccgtcgc gattcacgac
    22021 ggttttccgt cgagaacgga gggcatccat accacgttca gccgggagcg gcgagcttag
    22081 tctttgattt gatagaggca actgcctccg gtatttgccg agcgatcccg cgcattcgtc
    22141 cgcgttagaa ctgcccaccg ctcttcatgt actcgagctc ctcggcggtc gaggtgcgac
    22201 cgagcgcttc gttgcgacgc ggatagcgcc cgaaccggcg gatcgtatcg atgtggatgc
    22261 gctcgaactt cagcgcgttc tcatccccga gcacggtaaa gaggcgcatc gcttcttcgt
    22321 ggacgacgag cgactccgag tgcatgtacg gcatgtagag gaaggtgcgg cgaggtccct
    22381 cgagcttcgt atcgaccttc tgcgcgacgg cttcctgcga caggacgagc gccatcccgt
    22441 cgtacgcgaa cgcttggctg cttccgcgga agatgttgcg cgagaattga tcgaggacga
    22501 tgacctcggc gaggcggccc tctggcgttg cgcgccatcc gaacgtctcg ccgcgggcga
    22561 cggcatcgtg cgtcgcggag aacttgtcgc ggatctgcgc gtcgagctcc ttcgagccct
    22621 ggaaccaatc tgtgtgggtc agcccgaacc agaagtcgag gacgtcgtct gcgtgcttca
    22681 cggtcgcgac catacctctt ggcgcggcca cgatgatgat cgactatccg ccgcgatgag
    22741 gtttgcggca gtgtccctct cgatcgcgct gttggcatgc ggcgatgaca gctcgacgac
    22801 agcgccggat gcgccgatgg ccgatacgcc gacgacctgc acgcgcacgc cggccgctgc
    22861 cgatcgcaca cgcttcgtcg tcgtcgcaca tccgtacacg aggtcgggcg atccgtccgc
    22921 ggacttcgaa gtgctgcagc tgtcggcgac aggcacgctc acgcgattcg cgccgccgca
    22981 cacgttcacg ctcggcaagc gcgcgccgtt tggcgtgatc acgttcacgc ccgacggtgt
    23041 cgttggcatc gtcgcgctcg acgacggcaa gctcggcgtg ttcagcctcg acggcgatgg
    23101 caacgcgacg gtgatcgacg caggtgttgc aggcgagttc tacgccgaca gcgtcgtcat
    23161 ggatccatcg ggcgatcatg cgtgggtcct cgatgcgaac acgcgcgaga acggcggcgg
    23221 catctaccgc atctcgatcg actgcgacgg caaggtggcg gacaaaggca tcgtcgcgat
    23281 ggcgaagacg cccggaggtc tcgcgttcca tgacggccac gcggtcgtcc cggcgcacga
    23341 catcctcggt tctccgacga cgggcgacga cgttcatctg ctcgactggc cggcgaccgc
    23401 tgcggctccg acgctcctcg gcggtggcga cgcgttcggc gacgacgact cgatcttcag
    23461 cggcttcgcg ctctcgcacg acggtgcgac tgcgttcatc ggcgacagca atgtggtcgg
    23521 cacgaatcgc gtcggcatcg tgagcgtgag c
//
LOCUS       DATLBZ010000379        20791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6184121, whole genome shotgun sequence.
ACCESSION   DATLBZ010000379 DATLBZ010000000
VERSION     DATLBZ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20791
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6184121"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..822)
                     /locus_tag="VMZ53_19425"
     CDS             complement(151..822)
                     /locus_tag="VMZ53_19425"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS30694.1"
                     /translation="MVRRAAARSPDVDEDVLFKEPDGPEFRQERSRRSYQALLDAAEG
                     LFSAQGYDAVGTPEIAQKAGVSVGTFYRYFEDKHEVYLEIMRRTLAAAYNATIEKLKP
                     EQFVGKARHETISEGCAILFEHVIDKPQLTRSFMEMSLRDAQVAELRRAFEQLAVQRL
                     AGLIEAITPRSSVADPEAFAYVLYGAAMQCAYGLAVQLVPTQISRERAHAALTSFIER
                     ALFPS"
     gene            909..1940
                     /locus_tag="VMZ53_19430"
     CDS             909..1940
                     /locus_tag="VMZ53_19430"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUS30695.1"
                     /translation="MVATAETLTGPSIETLETMLDVTYTWGYQETRQKLRDLYDKAVR
                     GQWISDEVLPWQTDVDLEKPQGPEQLMPLFGSDIYMKMTEKERRNINIESFGWTLSQF
                     LHGEQGALLATAQLVDSVDNIDSKLYAASQVADEARHVDVYNRYVHKKIGFSYPCNPH
                     LKTLLDMILKDSRWDMKFLGMQIMVEGLALAAFGMIRNNTQEPLLKALTAYVMGDEAR
                     HVAFGVLSLRDYYREQPESVKREREDFVYEAARLMRDRFLFQEVWEKTGLPVKECMDI
                     ALNNAGQVMFRQMLFAKIVPAIKKMDLLSDRQRERFAQLGILQFENWADPFADSESSP
                     TGATSARLQ"
     gene            1968..2375
                     /locus_tag="VMZ53_19435"
     CDS             1968..2375
                     /locus_tag="VMZ53_19435"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HUS30696.1"
                     /translation="MIRALALSLFVVLVAGACEKKPAEKKAATPVEKSAPVTAGNTGS
                     DGLRKVAIEANKDGYTPDRIAGKPGEKLVLVFKRTIEADCLSQVKVADGAPVELPMNT
                     PVEVPVTVPQSGELAFACGMDMFHGTVVAAPNG"
     gene            complement(2895..6077)
                     /locus_tag="VMZ53_19440"
     CDS             complement(2895..6077)
                     /locus_tag="VMZ53_19440"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30697.1"
                     /translation="MPVDAAVEEQFGPYLVYERLGVGGMATVHRALEQGADGFERMVA
                     LKRLLPHLAEDASFIKSFVREAKLASLLNHVNIVQIYELGRVGTEYFITMEYIDGRDV
                     RRILRHARKVTGPPPIHVTVGLLLQLCDALDYAHSKVDEDGAPLGLVHRDISPSNLIV
                     TSGGHLKIIDFGIAKAQSSQLRTQTGRVKGKLAYMAPEAISGSRDLDARSDIWATGVI
                     LHELLTARPLFASKNEYQTLLKVKKGDILPPSTFNQACPPELDAIVFRALARHADERF
                     SNAAELREELQQLKRQYSLQTGYRDVASWVDWAFGLEPPPGFTGDTSGVMSVDKSLSH
                     SKTPRPPQNADEEEAVEVVWGTGAEESQEGMPVVLDEVPDVSEKVRTKRDTEDDRFGP
                     DDIPTPQPSHGTMPSIYGTNQNLVVLAPTTDPGLPPKLIDPALARGTDPPMARGSDHM
                     RAQMARPTQPQPLTAPRTLTGQSRVQQKTPAVGAPVAPAAKQPSAGVPMPAGSKIPTG
                     QMPAVRAKDASIPPQTQRPNRPTAPGVAPRQSDRIPTQAERPSDRMKAQTQKSQQMTA
                     VDVPNIDQRLAAVRGHDSIPPVDQNAHHHAQTPRALTLGDDSVSTNPIAKPLSAEELF
                     GIEAEQALSSVSAQIEAMATHTAMPAHRLDELPRAPIRDEVAQSAMPSVPVVRFSKTH
                     SIPPTNTTSTANKSMNSAYDPARIPSGNLPPASTATSGVDNARVPSTKPGIAPVNASA
                     APHIREPRRSKPPSNIQIGQTIAERAKPRRTWLFVVAGLLLAGGTAAVVSVMLTKSGD
                     NSTASAASDTPSSTTPGTGPTGTTPSAPIPTIGTIKFVTEPADAEIKIDSSTHAGSPF
                     TSELAAGIHQVEIRKPGFKSWLTSLELSGGEMQTLRVVLEPLAATPDSNATLMISTTP
                     PGLDVVIDGQPFKDKSPIKTTLTVGSHTVSVKQNGVEVWKQTVNAEASSDYEFNPSFT
                     DDKKRERAQEFRPKAPAPAPAPAPTPTPPAAPQPKADTPPAQPAAQPATPSAPTPAPP
                     PTAPAGAQPPATSLSSSPSAPTTTPIAPAQ"
     gene            complement(6183..6413)
                     /locus_tag="VMZ53_19445"
     CDS             complement(6183..6413)
                     /locus_tag="VMZ53_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HUS30698.1"
                     /translation="MKPEDIVAKIRVALPDAQVELQDLTGTADHWKAVIVSSAFAGKS
                     LMQRHRMINAALAEELKGPIHALTLEVKTPDE"
     gene            complement(6410..7597)
                     /gene="kbl"
                     /locus_tag="VMZ53_19450"
     CDS             complement(6410..7597)
                     /gene="kbl"
                     /locus_tag="VMZ53_19450"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HUS30699.1"
                     /translation="MYTNAKSVYAAQLAEIEQAGLWKHERLITSPQSSHITTNGKEVI
                     NFCANNYLGLSSHPRVMAAAKKALDERGYGLSSVRFICGTQDKHKELEAAVSKFLSTE
                     DTILYSSCFDANGGLFETVLGEQDAIISDALNHASIIDGIRLCKAERHRYEHDDLNDL
                     EAKLKATQSKRLRMVATDGVFSMDGDIARLDAICDLAEKYGAMVMVDDSHATGFTGKT
                     GRGSAELRGVLDRIDVFTSTLGKALGGASGGFTSGRKEIVDLLRNRSRPYLFSNTLAP
                     PIVAGSIEAIALLSESTALRDKLEANTKRFRAGMTQAGLAIREGTHPITPVMLGDAKL
                     AVDMAQRLLGHGIYVIGFSYPVVPKGQARIRVQISAAHSEADVDKAIAAFATVGRELG
                     IVK"
     gene            complement(7615..8670)
                     /locus_tag="VMZ53_19455"
     CDS             complement(7615..8670)
                     /locus_tag="VMZ53_19455"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HUS30700.1"
                     /translation="MRWLGLVVVTGCAVSPDLADGESSVVLGDNQMIRVAPDAANVPA
                     RYRSVLDAIGRSRPNYCTVTHLGNGIAITAGHCFAQQGRNKPCTSQSGDAINIDWGVR
                     GGDDASGYMTSRCMRILASEFHEQQTDYAIIEVSPAPAARAPFTFTRPSAATKLTIFS
                     HPNRRPLVWSQICERGRQVSPSVFAHDCDTDKGSSGAAVFVDSSFVIAGVHNGGHPGE
                     YNYGTYLADTPLAEFVSGGAPSGPVPDPTTHPTGGTDDDLSTGERFGETLAAGETLDL
                     GPYTVAAGHSLTITTEGVGDVDLYARRGAPPVGTSFDCGSAGPGANESCTIQGPASVY
                     AQVLGYAPSSSFDLLVQ"
     gene            complement(8817..9788)
                     /locus_tag="VMZ53_19460"
     CDS             complement(8817..9788)
                     /locus_tag="VMZ53_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUS30701.1"
                     /translation="MKILLTGAGGQIGHDLIGALVAQGHDVISTDLAPRPPSHAHAEG
                     VDWQRLDVTDAAAVQHTFTDVRPDLVFHLAAILSATGEANPRLAYDVNQTGTWNVLEA
                     CRRAKVGRLIFTSSIAVFGPPPSGPLPDPCPDDVALHPTTMYGATKVSGELLCAYYRS
                     RYGIDCRGVRFPGLISAAMPGGGSSDYALFMYVDAVRKGGYEAFARADTRIPLMYMPD
                     GVRALLELAFADKAKLTRTMYNIAAFSPRADEIANSVKRAIPEAKFTYAPDTVRQGIL
                     DSWPKSIDDAAARRDWGWKPKFDLDGMTDDLIPRVRRMLELGAILSH"
     gene            9968..11554
                     /locus_tag="VMZ53_19465"
     CDS             9968..11554
                     /locus_tag="VMZ53_19465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30702.1"
                     /translation="MRYCTSCLAIYRTDFERCPLDGGQLAMSAYDPLVGMTIGRYVIE
                     SKIGEGGMGRVYRAHRIDDPTQEVALKILLGDLAASNVMRQRFAREAEAARQLSHPNI
                     VGVLDFGHTPAGLPFLVMELVEGTNLALILQKGPMQGDRVIRIARQLCDALDHAHGRG
                     VIHRDFKPDNILVVNEGPEERVKIADFGLALSQNDETRLTMTGVACTPAYAAPEQLRG
                     GTIDSRVDLYALGTSMFEMLTGGMLPFESDLQGTIRLKLAGEAPNLATIVPDMQPALA
                     TLVARLLSHDADRRPRSARSLMIALDAALKAPKRKLRTQDLAPQTRGSIVTFPPTKAQ
                     RAATKRLRKSTRPSRRTYAALAVAAIMIGAALASIIRSHAPEAEARTIAQPSITMTVH
                     AQPIAATAPVETMPVVQPVVAKAPAAQPKLAMVSVPAQRLAVVKKPSRSVARPQRIKR
                     HVAKRAAPATMPDEPDAIIDDADVEDSAPGSDVDTSTQPPQPAPQAAAPPAPPANRDA
                     ILDSLPEQLQAGPVADDELK"
     gene            complement(11558..12571)
                     /locus_tag="VMZ53_19470"
     CDS             complement(11558..12571)
                     /locus_tag="VMZ53_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30703.1"
                     /translation="MRRTGLVALLVIAACSTDPDPTATQVYKFGPFSVPPGSEDTSKC
                     VQITLHNADYLYVNTVELTTGPGFHHSNWFFVPEHVFAGDDGVFTCDDRSFNQATAGI
                     LGGVFFAQSTQSEHDVQQFPAGTVIEVPPHSKLVSTIHLLNTGEDPLELTPHIDVTPI
                     LKADVVTKLMSVVFEYHPIGIPAQKQSRVTVECEIGDKHQALLGRAPDFNMYYALAHY
                     HKWGTRQTMEAVRPDGSATTIYSTGNSVGDSLGAMLDPTFSMSGYTKLRLSCDYLNNT
                     DHTLYYANSGSGEMCVFLGFSDSTYIWSGGSLDEDPMPGTDINGVMSFQRSCQVFAID
                     GQR"
     gene            complement(12619..13353)
                     /locus_tag="VMZ53_19475"
     CDS             complement(12619..13353)
                     /locus_tag="VMZ53_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUS30704.1"
                     /translation="MQSAASAPGHVPEALTASDRVREEWRARIAAEYTSAAITQTLVL
                     WLIQAGAPPDLIDAGLVIVGDELVHSRMSHEVYVAAGGTAPPAITREQLGLSRTSQPL
                     EHDILLAAVRVFCLGETVAVPLFSHLREPCTQPLARAALDRILRDEVRHRDFGWDLLD
                     WLLTTPLAEHIPALLDAHMPAMKRELEASYGVANPSVADDDGAMLESDRAWGLAPPRD
                     YAAILAQTVEKDYKPRFAARGLVTQL"
     gene            complement(13308..13856)
                     /locus_tag="VMZ53_19480"
     CDS             complement(13308..13856)
                     /locus_tag="VMZ53_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30705.1"
                     /translation="MAARTPRRSFATPFVVTLAAAPACYVQSGSGGTGPSQPPPQPPP
                     TQTSQAQPDQGPPPTVMVNPPRPTPDTATPTQPTQPTQPTQPTPPTPTNPAPQSSTQW
                     TVFKTSDGCQAAVKTECPKGVMCNPPPPIKYACPDNVTLPVTVISRDSGATCAIEFPM
                     PQCPQGAACNPPRPRPVTCPKR"
     gene            complement(13908..14774)
                     /locus_tag="VMZ53_19485"
     CDS             complement(13908..14774)
                     /locus_tag="VMZ53_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30706.1"
                     /translation="MRVAVIVALGVVAATMHARAETITDRAKRLFDEGLVLEDRGDSG
                     ACKLFEESYDLVAAAGTGFNLAECMERKGRVLRAWELYQAAATEWMRDAKDKRAAIAR
                     ERAAALEHKLVTIIIELANPDLELLTITLGERRLTPAPTIRALADPGRVEIRAVAPGY
                     VPYVDVAEASAGTTKQFRIELAAVAAEPPPIVRTQRRRSRIAVSVGLGGIAIGSLVAG
                     GVFFAQARQLQDDGFREGAVRRADLATVLGVAGGAIAIGAVVVYVTAPYDVTVIPIAS
                     TRSAGLVLSRAF"
     gene            14797..15921
                     /locus_tag="VMZ53_19490"
     CDS             14797..15921
                     /locus_tag="VMZ53_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30707.1"
                     /translation="MSHRLAVVVVVAVATSGCRYLLGDADEARDSGSNDSDTTNDPRC
                     GWGSSGLPQGGICLDELPSEPVSLGTSLDTDTSPLCVAYSKGGGTYCVIAGTTITIDS
                     VRVVGSKPLVVVATESVSVVGASDLASRTVGPPAAGSDPANGCDAGIGPNDGNNPETR
                     GGAGGSFGGRGGRGGLRNAQPPSPTSGVPGQPVQLAAPATLRGGCRGQDGFGAGGALG
                     GAGGGAVYVIAANQIDIATTGSLNASGAGGAGGAVRRGGAGGGSGGMIVLDALVVNIR
                     GAVFANGGGGGEGGGMNNPGNPGGDSDGTLAAPGGNGGAGEGSDGGAGSFQTMLDGSD
                     PAQTANTESGGGGGGAAGVIWIHATSGERAATTSPPAAAI"
     gene            complement(15926..17608)
                     /locus_tag="VMZ53_19495"
     CDS             complement(15926..17608)
                     /locus_tag="VMZ53_19495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30708.1"
                     /translation="MTWSEVGSSANRYEVLGKLAKGGMAEIFLARAASGAGVSKYVVV
                     KRVLPERAADLQLVRMFLDEARLAAQLQHPNIAQVYDVGKLGESYFFSMEYVHGVTIR
                     QLMVALRRNAMPPAIALGIGAGAAAGLHHAHERLGLDGRALGIVHRDISPSNLMLSFE
                     GHVKVVDFGIAKATTHTEQTREGVVRGKIAYLSPEQALGAPLDRRSDIFSLGIVLWEI
                     LAGRRLFKRPNDYASMAAVVEEAVPPLPAHVPAQTAELVMRALAKDPDDRYQTAQDML
                     DDIERVATSLGAAMPFTSLARFVRELYGPQPEPWHALNAQRDSNEAVLIAAEPIPDQL
                     TVVPSNAIERQLAAVADLSTTVGAVEELEVRRTTAVPLVVGDPDPPPSRGWLRTHWKA
                     VILIGATAVAVITAALIATQGYAPDQTPRLVTRVVEPTPTIDAQHEVIADAPVAVVEV
                     QVDAAASEVSDTPALETTPGPRRTTASPSRPSSDERQYSACADHGASKQPVNCTIVAC
                     RLRRQRAARAWFARISPAKQDAVIARCKTNGIVLRASDDAPDCDKDPSRCQF"
     gene            17654..20524
                     /locus_tag="VMZ53_19500"
     CDS             17654..20524
                     /locus_tag="VMZ53_19500"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HUS30709.1"
                     /translation="MSFRVRTAVIVATAFGAGACRDESCIDGRCPLPCAAIQLTCTDQ
                     PVFYQGTLGEAPANLRLVGGNAGAGDIMISNGRVTAVISAVDTPNDLAPTGGNLIDFG
                     VPGGLDDITLTYQIAGILPDDAFAYRTLEHAQTANGVTVTLRGTLDGRPDVKVATHYE
                     LRACDPGVRVRTELFNGSFDKQAFVIADAMHWGKRRQVPFVPATEQGYSQPELDLLEL
                     AAQWAPYDYATAVTPGASGPAYGTVGCSREQLEGVNDLEIAALGTPMEYVEPGDSLVL
                     ERMLVATGQGNGTAPAVDAIVDARAQLFDETPITVKGRIIVGTGHVVIPFGGDVRRAS
                     VVVRSNGRPVTSLVPALDGTFSARVPAGTIDIEVWSFGHLASKQAASVDDNGVAQLGD
                     VFVPEPATVQLSVTRHRSSAQAESAWAIAVFTQPGAEPGTFHGRLNGCAPWLGPPHGP
                     SPACNQVIVSPQGTELEVPAGRYVVYASAGPEHTLARQEVTLNAGEITPIAFDLTELS
                     LVPPSWLATDLHVHGRASFDSGIPDDDRVKTFVASGVQVIAATDHDVIGDYSQTVAAL
                     GLDDRVAVMGGLEATQLIPWMDIEGEDLPRVIGHFNFWPLTRMPSEPRAGAPWDELIE
                     PGQLFDRMQPLVGDDGMMMLNHPWGDPLFGRDTGYLRAIKFDPRKPIDDQPTLTKRPG
                     GLHRNMDWNIIEIINGADMSELAQSRVLWHSLLAQGIVVPGAGNSDSHGMTDEHLGWA
                     RNWVEAPTQVAGFSATTLNAAIRDGHMIAGNGVVVLVEVGPKTGQRRGLGFAPYHANA
                     GDVLAITVKAAPWIPVDEVRVVTSRGTKVVATNLAAPADPFGTDGVVRYTAELPLADL
                     VDRDDFIIVEAGLAYPLSADLNDDGVPDTTDNNRDGVVDTNDVDPDEDVGPYAGPPDP
                     TDPNDRLYVFTRILPTGWPEGFANPLFIDTDGGGWTPPGLAR"
     gene            20521..>20791
                     /locus_tag="VMZ53_19505"
     CDS             20521..>20791
                     /locus_tag="VMZ53_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30710.1"
                     /translation="MKSALAHIAITGIALAAPTWAHAEATDDERAAGEADSDDDVQAA
                     RADEPRVAADVEPLAVERVVPGEACDDAVIDPVVTPMRDAYLDAQR"
BASE COUNT         3447 a         6714 c         7087 g         3543 t
ORIGIN      
        1 aacagtgcag cagcgagcag gaaagaacgc atggtgctag agagccacga gatgcgtcga
       61 cggtgtacag caaaaatgcg ttgtcacatg cgtcgcaccc gtcgcgcgac acgatgagac
      121 atggtgcgac ggcggatcag atcgccgcga tcacgacggg aacagcgcgc gctcgatgaa
      181 cgacgtgagt gctgcgtgcg cgcgctcgcg cgagatctgc gtcggcacga gctgcaccgc
      241 gagtccatac gcgcactgca tcgctgcgcc gtagagcacg tacgcgaatg cttccggatc
      301 ggcgacgctc gagcgcggcg tgatcgcttc gatgagcccg gcgagtcgct gcacggcaag
      361 ctgctcgaac gcgcgccgca gctcggcgac ttgtgcatcg cgaagcgaca tctccatgaa
      421 gctgcgagtg agctgcggtt tgtcgatcac gtgctcgaac aagatcgcgc agccctccga
      481 gatggtctcg tggcgcgcct tgccgacgaa ctgctcgggc ttgagcttct cgatcgtcgc
      541 gttgtatgcg gccgccagcg tgcggcgcat gatctcgagg tagacctcgt gcttgtcttc
      601 gaagtagcga tagaacgtgc cgaccgagac gccggccttc tgcgcaatct caggcgtgcc
      661 gaccgcgtcg taaccctgtg cggagaacag cccctccgct gcatcgagga gcgcctgata
      721 gctgcggcgc gacctctctt ggcgaaactc ggggccgtcg ggttctttga acagcacgtc
      781 ctcgtcgaca tccggagacc gagcggctgc gcgcctgacc atgaatccag aatgcccttg
      841 acccgggacc gttcgctact acaaaattga aacaagtttc acttttggga catcgaaggg
      901 gacacaccat ggttgctacc gccgagacgc ttaccggccc gtcgatcgag accctcgaaa
      961 cgatgctcga cgtgacgtac acgtggggct accaggagac gcgccagaag ctgcgcgacc
     1021 tctacgacaa ggccgtccgc ggccagtgga tcagcgacga ggtcctgcca tggcagacag
     1081 acgtcgacct cgagaaacca caaggtcccg agcagctgat gccgctgttt ggctccgaca
     1141 tctatatgaa gatgacggag aaggagcgcc gcaacatcaa catcgagtcg tttgggtgga
     1201 cgctgtcgca gttcctccac ggcgaacaag gcgcgctgct cgcgacggcg cagctcgtcg
     1261 actcggtcga caatatcgac agcaagctct acgcggcatc gcaggtcgcc gacgaagccc
     1321 gccacgtcga cgtctacaac cgctacgtgc acaagaagat cgggttctcg tatccgtgca
     1381 acccgcatct caagacgctg ctcgacatga tcctcaaaga cagccgctgg gacatgaagt
     1441 tcctcggcat gcagatcatg gtcgaggggc tcgcgctcgc cgcgttcggc atgatccgca
     1501 acaacacgca ggagccgctc ctcaaggcgc tcaccgccta cgtgatgggc gatgaagcgc
     1561 gccacgtcgc attcggcgtg ctctcgctgc gcgattacta cagagagcag cccgagtccg
     1621 taaagcgcga gcgcgaagac ttcgtctacg aagccgcccg cctcatgcgc gatcggttcc
     1681 tgttccaaga agtctgggag aagacgggcc tgccggtcaa agagtgcatg gacatcgcac
     1741 tcaacaacgc gggccaagtc atgttccgcc agatgctgtt cgcgaagatc gtccccgcga
     1801 tcaagaagat ggacctgctc tccgaccgcc agcgcgagcg cttcgcgcag ctcggcatcc
     1861 tgcagttcga aaactgggcg gacccattcg ccgacagcga gtcgtccccg acgggtgcga
     1921 cgtccgcgcg cctgcagtag cgcggcacct ctgctatacg tgagcgcatg attcgcgcgc
     1981 tcgccctctc gctgttcgtc gtgctcgtcg ccggtgcctg tgaaaagaag cccgccgaaa
     2041 agaaggcggc gacgccggtc gagaagtccg cgcccgtcac cgcgggcaac acgggcagtg
     2101 atggccttcg caaggtcgcc atcgaggcga acaaggacgg ctacacgcca gaccgcatcg
     2161 cgggcaagcc aggcgagaag ctcgtgctcg tgttcaagcg gacgatcgag gccgactgcc
     2221 tctcgcaggt aaaggtcgcc gacggcgcgc cggtcgaatt gccgatgaac acgcccgtcg
     2281 aagttccggt cacggtgccg cagtccggcg agctcgcgtt cgcgtgcggc atggacatgt
     2341 ttcacggcac ggtcgtcgca gcgccaaacg gctagccgcc gcgcccccgc gcgccatcgc
     2401 cgccgatctg cgcgcctttg ctgatcgcag gatcggaagg gcggagagtt cgttcgcacg
     2461 ggtagggcgt gtgcccggat ggaacgcaag ggagcaagcg gagcaaggga cgcaagtccg
     2521 aggatcgggg cgccttcggc gcgttcgttc gcacgggcag ggctcgccgc gttcgcatgg
     2581 ctcggcgcgt tcgcatggct cgccggcggt tcgttcgcac ggcagggcgt gtgcccggat
     2641 ggaacgcaag ggagcaagcg gagcaaggga cgcaagccgg aggatcaggg cgccttcggc
     2701 gcgttcacac gcacggggat cgctcgagcg cgtaacgacc atcgagaatc gcgctctgcg
     2761 gggcgtgcgc gttccggcga ccacgttccc ccgatcgatc tctctgcttg cgtccctctc
     2821 tggccttgcc tccttgcgtt ccatctggga acgacgagca tcgtggcggc agcgacgagc
     2881 gtcgaggggg cggattactg cgctggcgcg atcggcgtcg tcgtcggcgc ggacggcgat
     2941 gacgagagcg acgtagccgg cggctgtgca ccggctggcg cagtgggcgg cggcgcgggc
     3001 gttggcgcgg agggcgttgc gggctgcgcg gcgggctgcg cgggcggcgt atcggccttg
     3061 ggctgtggag ccgcaggagg cgtgggcgta ggggcggggg ccggggcagg cgcgggcgcc
     3121 ttcgggcgga actcctgcgc gcgttcgcgc ttcttgtcgt cggtgaacga cggattgaac
     3181 tcgtagtcgc tcgaggcttc ggcgttgacg gtttgcttcc agacctcgac gccgttctgt
     3241 ttgacggaga cggtgtgcga gccgacggtg agcgtcgtct tgatcggcga cttgtctttg
     3301 aacggttggc cgtcgatgac gacgtcgagg cccggcggcg ttgtcgagat catcagcgtc
     3361 gcgttggagt cgggcgtcgc ggcgagcggc tcgagcacga cgcgcagggt ttgcatttcg
     3421 ccgcccgaga gctcgagcga cgtgagccat gacttgaagc cgggcttgcg gatctctact
     3481 tggtgaatgc cagcggccag ctcgctcgtg aatggcgagc ctgcgtgcgt cgacgagtcg
     3541 atcttgattt cggcgtcggc cggctcggtg acgaacttga tcgtgccgat cgtggggatt
     3601 ggcgcggacg gcgtggtgcc ggtggggccg gtgccgggcg ttgtcgaaga aggcgtgtcg
     3661 ctggcggcgg aggcggtcga gttgtcgccg gacttggtga gcatcacgct gacgacggcc
     3721 gcggtgccgc ccgcgaggag caggccagcg acgacgaaca gccacgtgcg acgcggcttt
     3781 gcgcgttcgg cgatggtttg gccgatttgg atgttggacg gcggcttgct gcgacgtggc
     3841 tcgcggatgt gcggcgcggc gctcgcgttg acgggggcga tgcccggctt cgtgctcggc
     3901 acgcgtgcgt tgtcgacgcc actcgtggcg gtcgacgcgg gcggtaggtt gcccgacgga
     3961 atgcgcgcgg ggtcgtacgc cgaattcatg ctcttgttcg ccgtcgacgt cgtgttcgtc
     4021 ggcgggatcg agtgcgtctt cgagaaccga acgacgggaa cactcggcat cgctgactgc
     4081 gcgacctcgt cgcggattgg agcgcgcggc agctcgtcga ggcggtgcgc cggcatcgcc
     4141 gtgtgcgtcg ccatcgcttc gatttgcgcc gacacgctgc tgagcgcttg ctcggcctcg
     4201 atgccgaaca gctcctcggc ggagagcggc ttcgcgatcg gattcgtcga gaccgagtcg
     4261 tcgccgagcg tgagcgcgcg gggtgtttgc gcgtggtgat gcgcgttttg atcgaccggc
     4321 gggatcgaat cgtggccgcg caccgccgcg agccgctgat cgatgttcgg cacgtcgacg
     4381 gccgtcatct gctggctctt ctgcgtctgc gccttcatcc gatcgctcgg gcgttcggct
     4441 tgcgtcggaa ttcgatcgct ctggcgcggc gcgacgccgg gggcggtggg gcgattcggg
     4501 cgctgcgttt gcggcgggat cgatgcgtct ttggcgcgga ccgccggcat ctggcccgtc
     4561 ggaatcttgc tgccggcagg catcggcaca cccgcgctcg gctgcttggc ggcgggcgcg
     4621 acgggcgcgc caactgcggg cgtcttctgc tgaacgcgag actgaccggt gagcgtgcgc
     4681 ggagccgtga gcggctgtgg ttgcgtcggc cgcgccatct gcgcgcgcat gtgatcgctg
     4741 ccgcgcgcca tcggcggatc ggtgccgcgc gcaagcgctg gatcgatcag cttcggcggc
     4801 aagcctggat cggtcgtcgg tgcgaggacg acgagattct gattcgtccc gtagatcgac
     4861 ggcatcgtgc catggcttgg ctgcggcgtc ggaatgtcgt cgggaccgaa gcgatcgtcc
     4921 tcggtatcgc gcttcgtgcg gaccttctcg gagacgtcgg gcacctcgtc gagcacgacg
     4981 ggcatccctt cttggctctc ctccgcgccg gttccccaca cgacctcgac ggcttcttct
     5041 tcgtccgcgt tctgcggcgg ccgcggcgtc ttcgagtgcg agagcgattt gtcgacggac
     5101 atcacgcccg acgtatcgcc ggtgaatccc ggcggcggct cgagcccgaa tgcccagtca
     5161 acccagctcg cgacgtcgcg gtagccggtc tgcagcgagt actggcgctt gagctgctgc
     5221 agctcctcgc gcagctcggc ggcgttgctg aagcgctcgt cggcatggcg tgcgagcgcg
     5281 cggaacacga tcgcgtcgag ctcgggcggg cacgcttggt tgaacgtcga cggcggaagg
     5341 atgtcgccct tcttcacttt gagcagcgtt tgatactcgt tcttgctcgc gaacagcggc
     5401 cgcgccgtga gcagctcgtg gaggatcacg ccggttgccc agatgtccga gcgcgcgtcg
     5461 aggtcgcggc ttcccgagat ggcctcgggc gccatatacg cgagcttgcc cttcacgcgt
     5521 cccgtctgtg tgcgcagctg gctcgactgc gccttcgcga tgccgaagtc gatgatcttg
     5581 agatggccac cgctcgtgac gatcaagttc gacggcgaga tgtcgcggtg aacgagaccg
     5641 agcggagcgc cgtcctcgtc gaccttgctg tgcgcgtagt cgagcgcatc gcagagctgg
     5701 aggagcaggc caacggtgac gtggatcggc ggcgggcccg tgaccttgcg cgcgtgacgg
     5761 aggattcgcc ggacatcgcg cccgtcgatg tactccatcg tgatgaagta ctcggtgccg
     5821 acgcgaccga gctcgtagat ctggacgatg ttgacgtggt tgagcagcga cgcgagcttg
     5881 gcttcgcgca cgaacgactt gatgaagctc gcatcctccg cgagatgcgg cagcaggcgc
     5941 ttgagcgcga ccatgcgttc gaagccatcc gcgccttgct ccaatgcgcg atgaaccgtg
     6001 gccataccgc caacgccaag ccgctcgtac acgaggtacg ggccgaactg ctcctcgaca
     6061 gcggcgtcga ccggcatgtc tcccaggatt caaagcttcc cacgtttcaa gaggacgcgg
     6121 catcgggaaa agccgcgaaa ttctagggac taacccgggc gctcccgctc agtgagacag
     6181 acttattcgt cgggtgtttt gacctcgagc gtcagcgcgt ggatcggacc cttcagctcc
     6241 tccgcgagtg cggcgttgat catgcgatgc cgctgcatga gcgacttacc cgcaaacgcc
     6301 gaagacacga tcaccgcctt ccagtgatcc gcagtgcccg tgagatcctg tagttccacc
     6361 tgcgcgtcgg gtagcgctac ccgaattttc gcgacgatgt cctcgggctt catttcacga
     6421 tgcccagctc gcgaccgacg gtggcaaacg cggcgatcgc cttgtcgacg tcggcttcgc
     6481 tgtgcgccgc cgagatctgg acgcgaatgc gcgcctgacc tttaggtacg acagggtagg
     6541 agaatccgat cacgtaaatg ccgtgaccga ggagccgctg cgccatgtcg acggcgagct
     6601 tcgcgtcgcc gagcatcacg ggcgtgatcg gatgcgtgcc ctcgcggatc gccaagcccg
     6661 cttgcgtcat gcccgcgcga aaccgcttcg tgttcgcttc gagcttgtcg cgcagcgcgg
     6721 tcgactcgga cagcagggcg atcgcttcga tcgagcccgc aacgatcggc ggcgcgagcg
     6781 tgttcgagaa caagtacggc cgcgagcgat tgcgcagcag atccacaatc tctttgcggc
     6841 cgctcgtgaa gccgccgctc gctccgccga gtgccttgcc gagtgtcgac gtaaacacgt
     6901 cgatgcggtc gagcacgccg cgcagctccg cgctgccgcg cccggtcttg ccggtgaagc
     6961 cggtcgcgtg gctgtcgtcg accatgacca tcgcgccgta cttctcggcg aggtcgcaaa
     7021 tcgcatcgag gcgcgcgata tcgccgtcca tcgagaacac gccgtcggtc gcgaccatgc
     7081 ggaggcgctt gctctgcgtc gctttgagct tcgcttcgag gtcgttgaga tcatcgtgct
     7141 cgtagcggtg gcgctccgcc ttgcacaggc gaatgccatc gatgatcgat gcgtggttca
     7201 gcgcgtccga gatgatcgcg tcctgctcgc ctagcacggt ctcgaacaac ccgccgttgg
     7261 cgtcgaagca cgacgaatag agaatcgtgt cctcggtcga caggaacttc gagaccgcgg
     7321 cctcgagctc cttgtgcttg tcctgcgtgc cacagatgaa gcgcacgctc gagagtccgt
     7381 aaccgcgctc gtcgagcgct ttcttcgccg cggccatgac gcgcggatgc gacgacaacc
     7441 cgaggtagtt gttcgcgcag aagttgatga cctctttgcc gttcgtcgtg atgtgcgacg
     7501 actgtggcga ggtgatcagg cgctcgtgct tccacaagcc ggcttgttcg atctcggcga
     7561 gctgcgctgc gtagacgctc ttcgcgttcg tatacatggc gcgcgcagcc tagctcattg
     7621 cacgagcaga tcgaagctcg acgaaggtgc gtagccgagc acctgcgcgt agaccgacgc
     7681 cggcccttgg atcgtgcagc tctcgtttgc gccgggccct gccgagccac aatcgaagga
     7741 tgttccgaca ggtggtgcgc cacgccgggc gtacagatcg acgtcgccga ccccttcggt
     7801 cgtgatcgtc agcgagtgac cggcggcgac agtgtacgga ccgaggtcga gcgtttcgcc
     7861 ggcggcgaga gtttcaccga agcgctcgcc ggtgctgagg tcatcgtcgg ttccgccggt
     7921 cggatgcgtc gtcggatccg gcaccggtcc ggacggcgca ccgcccgaga caaactcggc
     7981 aagcggcgtg tcggcgaggt acgtgccgta gttgtactcg ccggggtgac cgccgttgtg
     8041 aacaccggca atgacgaacg agctgtcgac gaagaccgcg gcaccgctcg agcctttgtc
     8101 ggtatcgcaa tcgtgcgcga acacactggg cgagacctgg cgaccgcgct cgcagatttg
     8161 cgaccagacg agcggacggc ggttcgggtg gctgaagatg gtgagcttcg tcgcggcgct
     8221 ggggcgcgtg aacgtgaacg gcgcgcgtgc cgcgggtgct ggcgagactt caatgatcgc
     8281 gtagtcggtc tgctgctcgt gaaactcact cgcaaggatg cgcatgcagc gcgaggtcat
     8341 gtagccgctc gcatcgtcgc cgccgcgcac cccccagtcg atgttgatgg cgtcaccgga
     8401 ttgcgacgtg cacggtttgt tgcgtccttg ttgcgcgaag cagtgaccgg ccgtgatcgc
     8461 gattccgttg ccgaggtgcg tgaccgtgca gtagttcgga cgtgatcgcc cgatcgcgtc
     8521 gagcacgctc cggtagcgcg cggggacatt cgccgcatcc ggggcgacgc ggatcatctg
     8581 gttgtcgccg agcacgacgg agctctcacc gtcggcgaga tccgggctga ccgcacatcc
     8641 cgtaaccacg accaagccaa gccagcgcat gcgcggcgtt gtacacgggc agcgcggagc
     8701 ccagaagtgt cgagcactgt cgtcgctttg cgcgacttgc gcggatcagc agggatagcg
     8761 agcgtcgctg aggtgcgcga tgcgcgtagc cgatgcgcag actgcgcatc cccgcgctag
     8821 tgagagagaa tggcgcccag ctcgagcatt ctgcgcacgc gcgggatgag gtcgtcggtc
     8881 atgccgtcga ggtcgaactt cggcttccaa ccccagtcgc ggcgcgcggc ggcgtcatcg
     8941 atcgatttgg gccacgagtc gaggatgcct tggcggacgg tgtcgggcgc gtacgtgaac
     9001 ttcgcctcgg ggatcgcgcg cttcacggag ttggcgatct cgtcggcgcg cggcgagaac
     9061 gcggcgatgt tgtacatcgt gcgcgtgagc ttcgctttgt ccgcgaacgc gagctctagt
     9121 agagcgcgca cgccgtcggg catgtacatg agcggaatgc gcgtatccgc acgggcgaac
     9181 gcttcgtagc cgcctttgcg aacggcatcg acgtacatga acagcgcgta gtcggacgag
     9241 ccaccgcccg gcatcgcggc ggagatgagg cccgggaagc gcacgccgcg gcagtcgatg
     9301 ccgtagcgcg accggtagta ggcgcacagc agctcgccgg agacttttgt cgcgccgtac
     9361 atcgtcgtcg ggtggagcgc gacgtcgtcg gggcaggggt cgggtagcgg gcccgacggc
     9421 ggcgggccga agaccgcgat cgacgacgtg aagatcaggc ggccgacttt cgcgcgccgg
     9481 caggcttcga ggacgttcca cgtgccggtt tggttcacgt catatgcgag gcgcggattc
     9541 gcttcgccgg tcgccgacag gatcgcggcg aggtgaaaaa cgagatcggg gcgcacgtcg
     9601 gtgaacgtgt gctgcacggc ggccgcgtcg gtcacgtcga gtcgctgcca gtcgacacct
     9661 tcggcgtgcg cgtggctcgg cgggcgaggc gcgaggtccg tcgagatcac atcgtgacct
     9721 tgcgcgacga gggcaccgat caagtcgtga ccgatctggc cgccagcacc cgtgaggagg
     9781 atcttcatgt gccgcgcatc ttgctgcacc gccgggagtg cttcaagagc acgcgggacg
     9841 aagcacggtg tagctgtggg tcacgtaaga gcgtacggct ttcagctgtt ttgccgagca
     9901 cagtcgttgc ttaggcagtt gcctcgtggc gacagcgctg gcaatgaccc cctcgtttca
     9961 gcccgccgtg cggtattgca ccagctgtct ggcgatctat cgcacggact tcgagcgctg
    10021 cccgctcgac ggtggtcagc tcgcgatgag cgcctacgac ccactggtgg gcatgaccat
    10081 cggtcgctac gtcatcgaga gcaagatcgg cgaaggcggc atgggccgcg tctaccgcgc
    10141 acaccgcatc gacgacccca cgcaggaagt cgcgctcaag atcctgctcg gcgatctcgc
    10201 ggcgtcgaac gtcatgcggc agcggttcgc tcgcgaggcc gaggccgcgc gccagctgtc
    10261 gcatccgaac atcgtcgggg tgctcgactt cggtcacacg cctgccggcc tgccgttcct
    10321 cgtgatggag ctggtcgagg gcacgaacct cgcgctcatc ctgcagaagg gcccgatgca
    10381 aggcgatcgc gtgatccgca tcgcgcgcca gctctgcgac gcactcgatc acgcgcacgg
    10441 ccgcggcgtc atccatcgcg atttcaagcc cgacaacatt ctcgtcgtca atgaagggcc
    10501 cgaagagcgc gtcaagatcg ccgacttcgg tctcgcgctc tcgcagaacg acgaaacgcg
    10561 cctgaccatg accggcgtcg cgtgcacgcc tgcgtacgcg gctcccgagc agctgcgcgg
    10621 cggcacgatc gactcgcgcg tcgacctcta cgccctcggc acgtcgatgt tcgagatgct
    10681 caccggcggc atgctgccgt tcgagagcga tctgcagggc acgattcgcc tcaagctcgc
    10741 cggcgaagcg ccgaacctgg cgacgatcgt tcccgacatg cagcctgcac tcgcgacgct
    10801 cgtcgcgcgc ttgctctcgc atgatgcgga tcgccgcccg cgttcggctc gctcgctgat
    10861 gatcgcgctc gacgctgcgc tcaaggctcc gaagcgcaag ttgcggacgc aagacctcgc
    10921 cccgcagacg cgcgggtcga tcgtcacgtt cccgccgaca aaggcgcagc gcgccgcgac
    10981 gaagcgtcta cgcaaatcta cgcgtccgag ccgtcgcacg tatgcggcgc tcgcggttgc
    11041 cgccatcatg atcggcgcgg cactcgcctc gatcatccgc agtcacgcgc ccgaagccga
    11101 ggcgcggacg attgctcagc cgtcgatcac gatgaccgtg catgcgcagc cgattgcggc
    11161 aacggctccc gtcgagacga tgccggtcgt gcagcccgtc gttgcgaaag cgccggcggc
    11221 tcagccgaag ctcgcgatgg tcagcgttcc ggcgcaacgc ctcgcggtcg tgaagaaacc
    11281 atcgcggagc gtcgcccggc cgcagcgaat caagcgccac gtcgcgaagc gagccgcgcc
    11341 tgcgacgatg ccggacgagc ccgatgcgat catcgacgac gcagatgtcg aagactccgc
    11401 gcccggctcg gatgtcgata cgtcgacgca gcccccgcag ccggctccgc aagcagccgc
    11461 gccgcctgca ccacccgcca atcgcgacgc gatcctcgat tcactaccag agcagctcca
    11521 agcggggcct gtggctgacg acgagctgaa gtagcgctta gcgctggccg tcgatcgcga
    11581 acacctgaca cgaacgctgg aagctcatca cgccgttgat gtcggtgccc ggcatcggat
    11641 cttcgtcgag cgagccgcca ctccagatgt acgtcgagtc cgagaagccg aggaacacgc
    11701 acatctcgcc actgccgctg ttcgcgtagt agagcgtgtg gtccgtgttg ttcaggtaat
    11761 cgcacgacag gcgcagcttc gtgtagccgc tcatcgagaa cgtcggatcg agcatcgccc
    11821 cgaggctgtc gccgacggag ttgcccgtcg agtagatcgt cgtcgcgctg ccgtcgggac
    11881 gtacggcctc catcgtctgg cgcgtgcccc atttgtgata gtgggcgagc gcgtaataca
    11941 tgttgaagtc aggcgcacgg ccgagcagcg cttgatgctt gtcgccgatt tcgcactcga
    12001 cggtcacgcg tgattgcttc tgcgccggga tcccgatcgg gtggtactcg aacacgacgc
    12061 tcatcaactt cgtgacgaca tccgccttca ggatcggcgt gacatcgatg tgcggcgtga
    12121 gctcgagcgg atcctcgccg gtattgagca agtggatcgt cgagacgagc ttggagtgcg
    12181 gcggcacttc gatgacggtg cccgcgggga actgctgaac gtcgtgctcg ctctgcgtcg
    12241 attgcgcgaa gaacacgccg ccgaggatgc cggccgtcgc ttggttgaac gagcggtcgt
    12301 cgcaggtgaa cacgccgtcg tcgcctgcga acacgtgctc gggaacgaag aaccagttcg
    12361 agtgatgaaa gccggggccc gtcgtgagct cgacggtgtt cacgtacagg tagtcagcgt
    12421 tgtgcagcgt gatctgcacg cacttcgagg tgtcctcgct accgggcgga acgctgaacg
    12481 gtccgaactt gtagacctgc gttgcggtgg gatccggatc ggtcgagcac gcagcgatca
    12541 ccagcagtgc gacgagcccc gttctccgca tgtcgttttg taccgctggt cgagtggccg
    12601 agccaatccc tattcacatc acaactgcgt tacgagccca cgcgctgcga accgcggttt
    12661 gtaatccttt tcaacggttt gcgcgagaat ggcggcgtag tcgcggggcg gagcgaggcc
    12721 ccacgcgcga tcactttcga gcatcgcgcc gtcgtcatcg gccacgctcg ggttcgccac
    12781 gccatagctc gcttcgagct cgcgcttcat cgcaggcatg tgcgcgtcga gcagtgccgg
    12841 aatgtgctcg gcgagcgggg tggtgaggag ccagtcgagc aggtcccagc cgaaatcgcg
    12901 atgccgaacc tcgtcgcgca agatgcgatc gagtgccgcg cgcgcgagtg gctgcgtgca
    12961 cggctcgcgc agatgcgaga acagcggcac cgcgacggtc tcgccgagac agaacacgcg
    13021 caccgccgcg agcaggatgt cgtgctcgag aggctggctc gtgcgactaa ggccgagctg
    13081 ctcgcgcgtg atcgccggcg gagccgtgcc gcctgccgcg acgtagacct cgtggctcat
    13141 gcgcgagtgc acgagctcgt cgccgacgat cacgaggccc gcgtcgatca gatccggcgg
    13201 cgcacccgct tggatgagcc agagcacgag cgtctgcgtg attgctgcgg acgtgtactc
    13261 ggcggcaatt cgcgcgcgcc actcctcgcg cacacgatcg cttgcggtca gcgcttcggg
    13321 cacgtgaccg ggcgcggacg cggcggattg cacgcggcgc cttgcgggca ctgcggcatc
    13381 ggaaattcga tcgcgcacgt cgcgccgcta tcgcgcgaga tgaccgtcac gggcagcgtc
    13441 acgttgtccg ggcacgcgta cttgatcggc ggcggcggat tgcacatcac gcccttcgga
    13501 cattccgtct tcaccgctgc ctgacacccg tccgacgtct tgaagaccgt ccactgcgtc
    13561 gacgattgcg gcgccggatt tgtcggcgtc ggcggcgtcg gttgcgtcgg ctgcgtcggc
    13621 tgcgtcggct gcgtcggcgt cgctgtgtct ggagtcgggc gcggcgggtt caccatcacc
    13681 gtcggcggcg gcccttgatc gggctgcgct tgcgacgtct gcgtcggcgg cggttgaggc
    13741 ggtggctgcg acggaccggt gccgccgctg cctgattgca cgtagcaagc gggcgccgct
    13801 gcgagtgtga cgacaaacgg tgtcgcgaaa gaacgacgag gcgtacgcgc tgccatgcgc
    13861 ggacgttata ccgcgggacg caaaaccacc attgtcatcg cgatggatca gaacgcacgc
    13921 gacagcacga gaccggccga gcgcgtgctc gcgatgggga tcacggtgac gtcatacggc
    13981 gcggtgacgt agacgacgac cgcaccgatg gcgatcgcgc cgccagcgac gccgagcacg
    14041 gtcgctagat ccgctcgacg cacggcaccc tctcggaagc catcgtcttg gagctgccgt
    14101 gcctgcgcaa agaatacgcc ccccgcgacc aggctcccga tcgcgattcc gccgagcccg
    14161 acggagaccg caatgcggct gcgccggcgc tgcgtgcgca cgatcggcgg gggttctgca
    14221 gcgacggccg cgagctcgat ccggaactgc ttcgtcgttc ccgccgacgc ttctgccacg
    14281 tcgacgtaag gtacgtagcc gggggcgacg gcgcggatct cgacgcgtcc tggatcggca
    14341 agcgcacgga tcgtcggagc aggcgttagc cttcgctcgc caagcgtgat cgtcagcagc
    14401 tccagatctg gattggcgag ctcgatgata atcgtcacga gcttgtgctc gagggcggct
    14461 gcgcgctcac gtgcgatcgc agcgcgcttg tccttggcat cgcgcatcca ctcggtcgcg
    14521 gccgcttgat agagctccca tgcgcgcaac acgcggccct ttcgctccat gcactcggcg
    14581 aggttgaagc cggtgcccgc ggcggcgacg aggtcatagc tctcttcgaa cagcttgcag
    14641 gcgcccgagt ctccgcggtc ctcgagcacc aacccctcgt cgaacagacg ctttgcgcgg
    14701 tctgtgatcg tctcggctcg cgcgtgcatc gtcgccgcga ccacgcccaa tgcgacaatt
    14761 accgccacgc gcacgtcgtt agagtacgct ggtttcatgt cgcatcgctt ggcggtcgtc
    14821 gtcgttgtcg ccgtcgcgac gagcggctgc cgctacctac tcggcgatgc agacgaagcc
    14881 cgcgactcgg gcagtaacga ctcggacacg acgaacgatc cgcggtgcgg ttggggcagc
    14941 tcaggcttgc cgcagggcgg gatttgcctc gacgagctgc cgagcgagcc cgtgtcactc
    15001 gggacgtcgc tcgataccga tacatcgccg ctctgcgtcg cgtactccaa aggcggcggc
    15061 acgtactgcg tgatcgccgg cacgacgatt acgatcgaca gtgttcgtgt cgtcggctcg
    15121 aagccgctcg ttgtcgtcgc gacggagagc gtgagcgtcg tcggtgcgtc cgatctcgca
    15181 tctcgtacgg tcggcccccc tgctgctgga agcgatccgg cgaatggctg cgatgcgggc
    15241 attggcccaa acgacggcaa caacccagag acacgcggcg gcgcaggcgg gagcttcggc
    15301 ggccgcggcg gacgcggcgg attgcgcaac gcacagccac caagcccgac gagcggtgtt
    15361 cctggacagc ctgttcagct cgctgctccg gcgacacttc gcggtggttg tcgcggccag
    15421 gatggtttcg gagcaggcgg agcgctcggt ggtgccgggg gaggcgccgt ctatgtgatc
    15481 gccgcgaacc agatcgatat tgcgacaacc ggctctctca acgcatccgg cgcaggcggg
    15541 gcaggtggtg ctgtgcggcg cggaggcgcg ggcggtggca gcggtggcat gatcgtcctc
    15601 gacgcgctag tcgtgaacat ccgcggcgcc gtgttcgcga acggaggcgg cggtggtgaa
    15661 ggtggcggca tgaacaatcc tggtaatccc ggaggcgact ccgacggcac gctcgcagcg
    15721 cccggtggca acggcggcgc aggtgagggc agcgacggag gtgcgggttc attccaaacc
    15781 atgctggacg gttcggaccc ggcacaaacc gcgaataccg agagtggcgg aggaggtggt
    15841 ggtgccgccg gcgtgatctg gatccacgcc accagtggcg aacgcgccgc cacgacctcg
    15901 ccgccggcgg ccgcgattta agcgctcaga actgacagcg cgacgggtct ttgtcgcagt
    15961 cgggcgcatc atcagaggcg cgcagcacga tgccgttcgt cttacagcgc gcgatgacgg
    16021 cgtcctgctt cgcaggtgaa atccgcgcaa accacgcccg cgccgcgcgc tgccggcgga
    16081 ggcggcacgc aacgatggtg cagttcaccg gttgcttgct cgcgccgtga tccgcgcacg
    16141 cggaatactg tcgctcatca ctcgacggtc gtgatggcga cgcggtggtt cgtcgcggac
    16201 ccggcgtggt ttcgagcgcg ggcgtgtcgg acacctccga tgccgcggcg tccacctgga
    16261 cctcgacgac cgcgacgggt gcatcggcta tcacctcgtg ctgcgcatcg atcgtgggtg
    16321 tgggttccac aacgcgcgtc acgagacgcg gtgtttgatc cggtgcgtac ccctgcgtcg
    16381 cgatcagcgc ggccgtgatg actgcgacgg cagttgcccc gatcaatatg acggctttcc
    16441 agtgcgttcg cagccaacca cgcgacggtg gcgggtcggg gtcaccgacg acaagcggca
    16501 ccgcggtcgt gcgccgcact tcgagctcct cgaccgctcc gacggtggtg ctcaagtctg
    16561 caacggcggc gagctggcgc tcgatcgcgt tgctcggcac gacggtcagt tggtcgggaa
    16621 tcggttcggc tgcgatcaag accgcttcgt tgctgtcacg ctgcgcattc aacgcgtgcc
    16681 acggttctgg ctgaggaccg tagagctctc gcacgaaccg cgcgagtgac gtgaatggca
    16741 tcgcagcgcc gagcgatgtc gcgacacgtt cgatgtcgtc gagcatgtcc tgcgctgtct
    16801 ggtagcggtc gtccggatcc ttcgcgagcg cacgcatcac cagctcggcc gtttgcgccg
    16861 gcacatgcgc cggaaggggc ggcacggctt cttctacgac ggctgccatc gacgcgtagt
    16921 cgttcgggcg cttgaacaag cggcggccgg cgagaatctc ccaaaggacg ataccgagtg
    16981 aaaaaatgtc gctgcggcgg tccagcggcg caccgagtgc ttgctcggga gacaggtacg
    17041 cgatcttgcc gcgaaccacg ccttcgcgtg tttgctccgt gtgcgtcgtc gccttcgcga
    17101 tcccgaagtc gacgaccttc acgtgtcctt cgaagctcaa catcaggttc gacggcgaga
    17161 tatcgcgatg cacgatgccg agcgcgcggc cgtcgagacc aagccgttcg tgcgcgtgat
    17221 ggagaccggc tgccgcgccc gcgcctatgc cgagcgcgat cgcaggaggc atcgcgttac
    17281 ggcgcagcgc gaccatcagc tgccggatgg tgacgccgtg gacgtactcc atcgaaaaga
    17341 agtacgactc gccgagctta ccaacgtcgt agacctgcgc gatgtttgga tgctggagct
    17401 gcgccgcgag cctggcttcg tcgagaaaca tgcgaacgag ctgcagatcc gcggcgcgct
    17461 ccggcaacac gcgcttgacg acgacgtact tcgagacacc ggctccgctc gcagcgcgcg
    17521 cgagaaagat ctcggccatg ccgcctttgg cgagcttgcc aaggacctcg tagcggttcg
    17581 cgctcgaacc gacctccgac cacgtcacgc tacggacagc ataacaaaca atgcatgtta
    17641 cagatggaac ggggtgagct tccgggtgcg cactgcagtc attgtcgcga cggcgttcgg
    17701 cgcgggcgcg tgccgcgacg agtcgtgcat cgacggaagg tgcccgctgc catgtgccgc
    17761 gatccagctc acgtgcacgg accagcccgt cttctatcag ggcacgctcg gcgaggcgcc
    17821 cgcgaacctg cgactcgtcg gtggcaacgc cggtgccggc gacatcatga tctcgaacgg
    17881 cagggtgacc gcggtcatct ccgccgtcga cacaccaaac gatctcgcgc cgaccggcgg
    17941 caacctcatc gacttcggtg tgccaggcgg tctcgacgac atcacgctca cgtatcagat
    18001 cgccggcatc ctgcccgacg acgcgttcgc gtatcgcacg ctcgagcacg cgcagaccgc
    18061 aaacggcgtg accgtcacgc tgcgcggcac gctcgacgga cgcccagacg tcaaggtagc
    18121 gacgcactac gagctgcgtg catgcgatcc aggtgttcgc gttcgcaccg agctgttcaa
    18181 cggcagcttc gacaaacaag cgttcgtcat cgcggacgcg atgcactggg gcaagcggcg
    18241 ccaagtgccg ttcgtgcccg cgaccgagca gggctacagc caaccggagc tcgatctcct
    18301 cgagctcgct gcgcagtggg caccatacga ctacgcaacc gcagtcacgc ccggagcgag
    18361 cggcccggca tacggcacgg ttggctgcag tcgcgagcag ctcgaaggcg taaacgatct
    18421 cgagatcgcg gcgctcggca cgccgatgga atacgtcgag ccaggtgact cgctcgtgct
    18481 cgaacgcatg ctcgtcgcga cggggcaggg caacggcacc gcgccggccg tcgacgcgat
    18541 cgtggacgcg cgcgcgcagc tgttcgacga gacgcccatc acggtgaagg gccgcatcat
    18601 cgtcggtacg gggcacgtcg tgatcccttt tggcggcgac gtccgccgcg catctgtcgt
    18661 cgtgaggtcg aacgggcggc ccgtcacatc gctcgttccg gcgctcgatg gaacgttctc
    18721 ggcgcgcgtc ccggcgggaa cgatcgacat cgaggtctgg tcgttcggtc acctcgcgtc
    18781 gaagcaagca gcctcggtcg atgataacgg cgtcgcgcag ctcggcgatg tcttcgttcc
    18841 cgagccagcg acggtgcagc tgtcggtgac gcggcatcgc tcgagcgctc aggccgagag
    18901 cgcgtgggcg atcgccgtct tcacgcagcc gggcgccgag ccgggaacgt tccatggccg
    18961 cctcaacggc tgcgcaccat ggctcggccc gccgcacgga ccgtcacccg catgcaatca
    19021 agtgatcgtc tcgccacagg gcacggagct cgaggtgccg gcaggccgct atgtcgtgta
    19081 cgcgagcgcg gggcccgagc acacgctcgc gcgccaggag gtgacgctca acgccggcga
    19141 gatcacgccg atcgcgttcg atctcaccga gctgtcgttg gtgccaccga gctggctcgc
    19201 aacggatctg cacgtccacg gccgcgcgag cttcgactcg ggaattcccg acgacgatcg
    19261 cgtgaagacg ttcgtcgcgt cgggcgtgca agtcatcgcc gcgaccgatc acgacgtcat
    19321 cggcgactac tcgcagaccg ttgccgcgct gggtctcgac gatcgcgtcg ccgtgatggg
    19381 cggcctcgaa gcgacgcagc tcatcccgtg gatggacatc gagggcgaag acctgccgcg
    19441 cgtgatcggg cacttcaact tctggccgct gacgcgcatg ccgagcgagc cgcgcgccgg
    19501 ggcgccatgg gacgagctga tcgagccggg ccagctgttc gaccgcatgc agccgctcgt
    19561 cggagacgac gggatgatga tgctgaacca tccgtggggc gatccattat tcggccgcga
    19621 caccggctac ctgcgcgcga tcaagttcga tccgcgcaag cccatcgacg atcaaccgac
    19681 gctgacgaag cgcccaggcg gccttcaccg caacatggac tggaacatca tcgagatcat
    19741 caatggtgcc gacatgagcg agctcgcgca gtcccgcgtg ctctggcact cgctgctcgc
    19801 gcaaggcatc gtcgttcccg gcgcgggcaa cagcgattcg cacggcatga ccgacgagca
    19861 tctcggttgg gctcgcaact gggtcgaggc cccgacgcaa gtcgcgggct tctccgcgac
    19921 gacgctaaac gccgcgatcc gcgacggcca catgatcgcg ggcaacggcg tcgtcgtgct
    19981 cgtggaagtc ggtccgaaaa ccggacaacg acgcggcctc ggcttcgcgc cgtatcacgc
    20041 aaacgccgga gacgtcctcg cgatcacggt gaaggccgcg ccatggattc ccgtcgacga
    20101 agtgcgcgtc gtgacctcgc gtggcacgaa ggtcgtcgcg acaaaccttg ccgcgcccgc
    20161 cgatccgttc ggcaccgatg gtgtcgtgcg ctacacggcg gagctgccac ttgcggatct
    20221 cgtcgatcgc gatgacttca tcatcgtcga agcgggcctc gcgtacccgc tgtccgcgga
    20281 cctcaacgac gacggcgtgc ccgacacgac ggacaacaac cgcgacggcg tcgttgatac
    20341 gaacgacgtg gatcccgacg aggatgtggg gccatacgcc ggtccgccgg acccgacaga
    20401 cccaaacgac cggctatacg tgttcacccg gatcctgccg accggctggc ccgaaggttt
    20461 cgcgaaccca ctgttcatcg acacggatgg tggcggctgg acaccaccgg gcctcgcaag
    20521 atgaagtcgg cgctcgcgca tatcgcgatc acgggcatcg cgctcgcggc gcccacgtgg
    20581 gcacacgcag aggcgacaga cgatgaacgg gcggccgggg aggccgatag cgatgacgat
    20641 gtgcaggccg cgcgcgccga cgagccgcga gtcgcagcgg atgtcgagcc gctggcggtc
    20701 gagcgagtcg tgcctggcga ggcgtgcgac gacgcggtca tcgatccggt ggtgacgccg
    20761 atgcgcgacg cctacctcga tgcgcaacgc a
//
LOCUS       DATLBZ010000380         4286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9384714, whole genome shotgun sequence.
ACCESSION   DATLBZ010000380 DATLBZ010000000
VERSION     DATLBZ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9384714"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VMZ53_19510"
     CDS             <1..769
                     /locus_tag="VMZ53_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30711.1"
                     /translation="QALTTYEGDAHLLFTGDLIHGPHIEPEDWPDFLGEYYRDASGEV
                     MIAYAGLAAQYPGKVHALLGNHEHGHIGGPHTAKFAADEVALLEHILGPAGTARMRGI
                     IHTFYLAAVSKCGAVFTHGAPAAQCESVADVEAADLSGAGLSSPLDVLDTPIVGKILW
                     ARSATEAEARRFLRAMNGTMAIYGHDVIPEGYETVGDEQIIVSTSFGLFDQNKVYLSL
                     DLSHKYRGVHDLRVGHEILPLYPEKAPARLGGRAKAS"
     gene            842..1057
                     /gene="rpmE"
                     /locus_tag="VMZ53_19515"
     CDS             842..1057
                     /gene="rpmE"
                     /locus_tag="VMZ53_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HUS30712.1"
                     /translation="MKDTAGHPHYRPAKITCACGTLHETFSTRGDFGVDICSACHPFF
                     TGKQKLMDTAGRIDRFNKKYAKTTPKS"
     gene            1088..2179
                     /gene="prfA"
                     /locus_tag="VMZ53_19520"
     CDS             1088..2179
                     /gene="prfA"
                     /locus_tag="VMZ53_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HUS30713.1"
                     /translation="MFESKNFNEKMTSLTRRHEEVSALLGTPEVINKRAEFLKLSREH
                     SELDPLVAAWKAYKKLVGDLTKARQMIEAESDAELRELAREEAKALDEQREKAEQELK
                     ILLLPKDPNDGKNAILEIRGGTGGDEAALFAGDLYRMYTRYAERQSWKIEVMSMSEGS
                     SGGVKEVVCLIEGKDVFSKLKFESGVHRVQRVPETEAQGRIHTSAATVAVLAEADEVD
                     VTIREADLKIDTYRSSGAGGQHVNTTDSAVRITHMPTGVVVACQEERSQIKNRAKAMK
                     MLRSKILEAEQQKQAQAEAAARKTQVGSGDRSEKIRTYNFPQDRVTDHRIGLTKHNLP
                     AIMDGGLDDIIEALRAHDQAEALKAQAAS"
     gene            2293..2589
                     /locus_tag="VMZ53_19525"
     CDS             2293..2589
                     /locus_tag="VMZ53_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30714.1"
                     /translation="MILDNGPTIEAYPMFDDHVAGGAPRVIDLRRGERFVGDVKRRFM
                     QGGIECVATASVRIAKCKSDALHLVIADPPSPLKFSPCEWPRPNEPELDHWHRE"
     gene            2609..>4286
                     /locus_tag="VMZ53_19530"
     CDS             2609..>4286
                     /locus_tag="VMZ53_19530"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS30715.1"
                     /translation="MKRVIAVGIAVLLVAVLVAWKACGSSSSHSSSAKSGSGAHSSSV
                     VASKLNKDGPVQPASIAGRVTRAQDGAPVAGATVSLAPAELMAMFIKSDMPTLVATTD
                     ANGNFKAATVMPGAYVVAATASGYLPGAHTKITVQQGEARTGIDIALTQGGTIVKGTV
                     TDVGGGAIGDARITANKGSEIPDLSGHSEFVVITKPDGTYEITLPDGEFRLEASHDDY
                     TSAGHRLEVAGKPLTQDFQLIPGAVIRGTVIAKDTGKPVPGALVRAEGGERGTRGGDD
                     GGTTIADADGNFTIRSLGSGTLQLNALGRGYASPQAGTAVSVGIGEQVDGVRVLVERA
                     YSISGRVVKKGKPDEGVPGITLGAFSIAAKAFGLAIEPSGPDGSFEIYGVKPAAYMLF
                     AVGEGTVPEIGKNVEVVDKDVTGVIVEMTAGVTVSGVVSPPLPGALITVELEGDFNMA
                     RMFEMAKLVLIRGETDQAGAFTLKNVPPGAFKLNAGAPDGHTGEQPVVVADTDKAGIV
                     IKLEPRASVSGRVTDSNGQPVAGGRVNVDPLDPDADKKMNISFNDMRNRNS"
BASE COUNT          825 a         1371 c         1421 g          669 t
ORIGIN      
        1 gcaggcgctg actacctacg agggtgacgc ccatctgctc ttcacgggcg atttgatcca
       61 cggcccgcac atcgagcccg aagactggcc cgacttcctc ggcgagtatt accgggacgc
      121 gtcgggcgaa gtgatgatcg cgtacgccgg attggctgcg cagtatccgg gcaaggttca
      181 cgcgctgctc ggcaatcacg agcacggtca catcggcggg ccgcacaccg cgaagttcgc
      241 cgccgacgag gtcgcgcttc tcgagcacat cctcggtccc gccggcacgg cgcgcatgcg
      301 cggcatcatc cacacgttct atctggcggc cgtcagcaag tgcggcgcgg tcttcacgca
      361 tggtgcgccg gcggcgcagt gcgagtcggt cgcggatgtc gaggccgcgg acctgtcggg
      421 tgcggggctc tcgagcccgc tcgatgttct cgacacgccg atcgtcggca agattctctg
      481 ggcgcggtcc gcgaccgagg ccgaggcccg ccgattcctg cgtgcgatga acggcacgat
      541 ggccatctac ggccatgacg tcattcccga gggctacgag actgtcgggg acgagcagat
      601 catcgtctcc acgagctttg gcctgttcga ccagaacaag gtctacctct cgctcgattt
      661 gtcgcacaag taccgtggtg tccacgactt acgcgtcggc cacgagatct tgccgctgta
      721 tcccgagaag gcgcccgccc gcctgggtgg ccgggctaaa gcgtcgtaac ggctaggcat
      781 ggactacccg gttttgggtg cttgcaatcg gggcgctccg tccctatatc tacgcgctgc
      841 tatgaaggac accgcaggtc acccgcacta ccgcccggcc aaaatcacct gtgcttgcgg
      901 aacgctccac gagacgttct cgacgcgcgg tgacttcggc gtcgacatct gctcggcttg
      961 tcacccgttc ttcacgggca agcagaagct gatggacacc gctggtcgca tcgatcgctt
     1021 caacaagaag tacgcgaaga cgaccccgaa gtcgtaaccc cgcaacgcgc ggcagcctga
     1081 ggccgccatg ttcgagtcga agaatttcaa cgagaagatg acgtcgctca cgcggcgtca
     1141 tgaagaagtc agcgcgctgc tcggtacacc cgaggtcatc aacaagcgcg ccgagttctt
     1201 gaagctctcg cgcgagcact cggagctgga tccgttggtc gcggcgtgga aggcgtacaa
     1261 gaagctcgtc ggcgatctga ccaaggcgcg tcagatgatc gaagccgaga gcgacgcgga
     1321 gctgcgcgag ctcgcccgag aagaagcgaa ggcgctcgac gagcagcgcg agaaggccga
     1381 gcaggagctg aagatcctgc tgctgccgaa agatccaaac gacggcaaga acgcgatcct
     1441 cgagatccgc ggcggcaccg ggggcgacga agccgcgctg ttcgcgggcg acctctaccg
     1501 catgtacacg cgctacgccg agcggcagag ctggaagatc gaagtcatgt cgatgtcgga
     1561 gggctcgtcg ggcggcgtga aggaagtcgt ctgtctcatc gaaggcaagg acgtcttttc
     1621 caagctcaag ttcgagtcgg gcgtgcatcg cgtgcagcgc gtgccggaga ccgaggcaca
     1681 gggccgcatc cacacgtcgg cagcgacggt ggccgtgctc gccgaagccg acgaagtcga
     1741 tgtgacgatc cgcgaggcgg acctcaagat cgacacgtat cggtcgagcg gcgccggtgg
     1801 ccagcacgtc aacacgaccg actcggcggt gcgcatcacg cacatgccga cgggcgtcgt
     1861 cgtcgcgtgc caggaagagc gctcgcagat caagaaccgc gcgaaggcga tgaagatgtt
     1921 gcgctcgaag atcctcgagg ccgagcagca gaagcaggcg caggccgaag ctgccgcgcg
     1981 caagacgcag gtcggctccg gcgatcgcag cgagaagatc cgcacgtaca acttcccgca
     2041 ggatcgcgtg accgatcacc gcatcggtct gacgaagcac aacttgcccg cgatcatgga
     2101 cggcggtctc gacgacatca tcgaggcact ccgcgcgcac gaccaagcag aagcgctcaa
     2161 ggcccaggct gcctcgtgaa gctgctcgtc gctgtcctcg ctgtcgcggc ttgcgatcgg
     2221 cgggcagcgg tgacgacctg cgatgacgat ctgcacggcg tgtggatcag cgaagagcac
     2281 gacacgcgct ggatgattct cgacaacgga ccgaccatcg aggcgtaccc gatgttcgat
     2341 gaccacgttg ccggcggcgc gccgcgcgtg atcgacttgc gcagaggcga gcgcttcgtg
     2401 ggcgacgtga agcggcgctt catgcagggc ggcatcgagt gcgtcgcgac cgcgtcagtc
     2461 cgtatcgcaa aatgtaaatc ggacgcgtta catctcgtga tcgccgaccc gccttcaccg
     2521 ctgaaattct cgccgtgtga atggcctcgg cccaacgagc ccgaattaga tcactggcac
     2581 cgcgagtaaa cgtgattaga cttcgcgcgt gaaaagggtc atcgcggttg gcattgctgt
     2641 cctcttggtg gccgtgctcg tcgcctggaa ggcctgcggt tcatcgagca gtcattcgag
     2701 cagtgcgaag agcggaagtg gcgcgcatag ctcgagcgtc gtcgcatcga agctgaacaa
     2761 ggacggtccc gtccagcctg catcgatcgc gggccgcgtg acgcgtgcgc aagatggtgc
     2821 gcccgtcgcc ggagccacgg tgtcgctcgc acctgccgag ctgatggcga tgttcatcaa
     2881 gagcgacatg ccgacgctcg tcgcgacgac cgacgcgaat ggcaacttca aggcggcgac
     2941 cgtgatgccc ggggcatacg tcgtcgcggc aacggcgagc ggctatctgc cgggcgcgca
     3001 cacgaagatc acggtgcaac aaggcgaggc gcgcaccggt atcgatatcg cgctcaccca
     3061 aggcggcacg atcgtgaaag gcaccgtgac cgacgtcggc ggcggcgcga tcggcgacgc
     3121 gcgcatcacc gcgaacaagg gcagcgagat tccagatctc agcgggcact ccgagttcgt
     3181 cgtgatcacg aagccggacg gcacgtacga gatcacgctg cccgacggag aattccggct
     3241 cgaggcctcg cacgacgact acacgagcgc gggccatcgc ctcgaggtcg cgggcaagcc
     3301 gctcacgcaa gacttccagc tgattcccgg cgcggtgatt cgcggcaccg tgatcgcgaa
     3361 agacacgggc aagccagttc ccggtgcgct cgtgcgcgcg gaaggtggcg agcgcggcac
     3421 gcgtggcggc gatgacggag gcacgacgat cgcggacgcc gacggcaact tcacgatccg
     3481 cagcctcggc tccggcacgc tccagctcaa cgcgctcggt cgcggctacg catcgccgca
     3541 ggcaggcact gcagtcagtg tcggcatcgg cgagcaagtc gatggcgtgc gcgtgctcgt
     3601 cgagcgggcg tattcgatct cgggtcgcgt cgtgaagaaa ggcaagcccg acgagggcgt
     3661 ccctggcatc acgctcggcg cattctcgat cgccgcgaag gcgttcggtc tcgcgatcga
     3721 gccaagcggt cccgatggct cgttcgagat ctacggcgtg aagcccgccg cgtacatgct
     3781 gttcgcggtc ggcgaaggca ccgttccgga gatcggcaag aacgtcgagg tcgtcgacaa
     3841 ggacgtcacc ggcgtgatcg tcgagatgac ggcgggcgtc accgtctccg gcgtcgtgtc
     3901 gccgccgctg cccggtgcgc tgatcaccgt cgagctcgag ggcgatttca acatggcgcg
     3961 catgttcgag atggcgaagc tcgtgctgat ccgcggcgag accgatcaag caggcgcgtt
     4021 cacgctgaag aacgtgccgc ccggtgcatt caagctcaac gcaggcgcac ccgacggcca
     4081 caccggcgag caaccggtcg tcgtcgccga cacggacaaa gccggcatcg tgatcaagct
     4141 cgagccgcgc gcatccgtca gtggccgcgt caccgattcg aacggccagc ccgtcgcagg
     4201 cggccgcgtt aacgtcgatc cgctcgatcc cgatgcggac aagaagatga acatctcgtt
     4261 caacgatatg cgcaaccgca acagcg
//
LOCUS       DATLBZ010000381         7762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7673447, whole genome shotgun sequence.
ACCESSION   DATLBZ010000381 DATLBZ010000000
VERSION     DATLBZ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7673447"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VMZ53_19535"
     CDS             <1..454
                     /locus_tag="VMZ53_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30716.1"
                     /translation="LSQICGALHEAHGRGVRCRDIKPANVMLCRRGGIPDFVKVLSFG
                     LTADHTSDAAPVVEGVAMLAKFLLGTDRVIPPALSALVDDCLAGRLTSASELADRLAA
                     LELGATWTDEQANAWWSTHNGASSPSGRASTPGLPQHVLEIDLAEHRA"
     gene            complement(458..616)
                     /locus_tag="VMZ53_19540"
     CDS             complement(458..616)
                     /locus_tag="VMZ53_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30717.1"
                     /translation="MTKLDNQSLETVTGGVAVLPIQPPGPRLPNGQAPPKYSAPWWDA
                     VRSSRIGK"
     gene            complement(744..2936)
                     /locus_tag="VMZ53_19545"
     CDS             complement(744..2936)
                     /locus_tag="VMZ53_19545"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30718.1"
                     /translation="MRCYWLTIAVIVGGCGRIGFDLLDPGDTLPPVDDCTPVTTALGV
                     GAGGNYTCVNAPGGLRCWGDGSEGQMGDGTRTSRIDPYFLPLGNVIAFATGAAHACAV
                     VGNALYCWGRNESGQLGIGNLVQQTSPTAVSNAPASIAGVAAGGMNTCVWTDAGELWC
                     WGRYPGVNTQSNQPVHVLGVPPIIDAALSSLDAVLSATHACAVGRDGTVWCWGTNDKG
                     QLGDNTTTLTMVPVQTVGLSTAIHVALASKASCAIVAGGDVYCWGINDTGQLGNGTRT
                     DSLIPVKASIADAVEIAAKADTFCVRKSDRTVWCWGANSDGQAGNADLRSSDVPRQVA
                     VPFAQAISVGVSHACAISTQGTLWCWGSDRVGERGDDATIDRTALFETGMNASQFASG
                     EDFTCAVPAGVAGQPLRCWGVNSSGQLGDGTKIAHSTPAPAMIDQVINVEAGNASACA
                     IRSDRTAWCWGDNDAENVAMGAAHPQTTPLAISLPQNVNYIAPATSSTCAVLSDSTTW
                     CWGANNEGQSALPNNMMGPLPPTNSNFPALSYVDAGAAHTCGLATAAAYCAGRGNELQ
                     IGEPDRTTDAYTPYMIGIANISNIQAGGLFTCALAASGMYCWGDNRRGQIGIDTTKNA
                     NPTLVGLSGVTAFAAGPHHVCAVNNLAEWSCWGDNSAGQLGDGTTTTHLSPVRAPLFD
                     GATKLTLGRTHTCMNGTAGLRCIGSFTWGPIGSPQPVTPTAANFSCPR"
     gene            3044..3772
                     /locus_tag="VMZ53_19550"
     CDS             3044..3772
                     /locus_tag="VMZ53_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30719.1"
                     /translation="MKTLLAATLLAALSATAFAQGADPQAPQPAPDAQPAVGDSTTTQ
                     PCSQALQQPPCATPTVAYAEPAPLTPQPVLDTPQVDDDGGWHKPVGRRFLHGFRLGWT
                     YLYNVEKMRLDSKGMPTVESLKDKYGIKSANMMLLGYEGFYRIIGHSWLNVLMVGNVT
                     VAGLEQSKFIPAASGLIGFEFNRSFQLGVGVNLTPDPHAPSHMIAAAGWTPNVGSIQI
                     PLHFFFVPDTDSNHRMGATIGMTW"
     gene            complement(3773..4636)
                     /locus_tag="VMZ53_19555"
     CDS             complement(3773..4636)
                     /locus_tag="VMZ53_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30720.1"
                     /translation="MRGVIVEVSSRKYGVPYECPCCGAAPDAEMRVTSETGHALDTPY
                     CKRCVGHVDKWEWAGIRAMGMAVGGLVLAIVLALVVSFLVAAIVLVIAIAAAAYLRSS
                     GKASAKHACGASCASPQVALAYGGWNGTTHSFTFESPTFAARFAEQNAAILANETPQL
                     RKLLDGYKKARLAVPTPAVAAGVAPPPLTARDWIARIESTDGKVARRVALGRALEMID
                     QPQPRRELVQTVARLELAPMLDKLQRTSSPAAKKALLQTSIQQLRNENVSDELEAALT
                     TALEARVKELG"
     gene            complement(4672..5580)
                     /locus_tag="VMZ53_19560"
     CDS             complement(4672..5580)
                     /locus_tag="VMZ53_19560"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUS30721.1"
                     /translation="MNRREFIQLTAAAAALLSTRAYGADKKKMTIHTRPIPRTKEELP
                     VVGLGTWQTFDVGAGADVRKPIDDVLRTFLDAGCRLIDSSPMYGKAEEVTGDELDAIG
                     AIGKPFLATKVWTSGKQAGIDAMQRSMKRMRAKRMDLMQVHNLLDYKTHLPVLRDWKD
                     AGTIRYLGVTHYQHDQFDLIEKLMKNEKLDFIQIPYSIVDRKAEARILPAAADTGTAV
                     LVMTPFESGALFRQVKGKPLPAIAKDIDCTSWAQYFLKFIIGHPAVTCPIPATSNPKH
                     IADNVLAAVGRIPDEAQRKDMAAHLV"
     gene            complement(5577..6479)
                     /locus_tag="VMZ53_19565"
     CDS             complement(5577..6479)
                     /locus_tag="VMZ53_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30722.1"
                     /translation="MKSLVIALVVVVLAGCGGKKKKEDSQVVDAYVNVDLAPQLAKVV
                     AARSAYGSVRAVDIDEQPEKMKWLFRDVAAPFLKEAVAGASVLTPPPAAKQLHEDTLG
                     LWKRESDIISAMAAATDPVDSAKFKEAHAQMMDLQESVMSWDRKLQTLLDASGGLKLK
                     PLPDVKIPEPKLDAPDPIAVAAGSAAPAGPNGVPAMCKAGTDVAQDDGSLVCETEMMF
                     ANPETPAGTPKEQVVACGPGRVVYDKAGKVVSCVSFASVTVGDTKLDKGTTITFGANA
                     SIREAVLPDGKQLCFENAKQVDCP"
     gene            6521..7726
                     /gene="purT"
                     /locus_tag="VMZ53_19570"
     CDS             6521..7726
                     /gene="purT"
                     /locus_tag="VMZ53_19570"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HUS30723.1"
                     /translation="MKPVSFGTPKSPHATRVLLLGSGELGKEVVIELQRFGVEVIACD
                     RYADAPAMQVAHRSHVIDMLDRAALRAVIEQEKPHLIVPEIEAIATAELVELEREGWN
                     VIPTAQAARLTMDREAIRRVAAEELKLPTARYQFADTFEELQSASRALGWPVVIKPIM
                     SSSGKGQSLAHTEADLEKSWHYAQTGGRTGAGRVIVEEFIKFDSEITMLTVRAANGTF
                     FCDPVGHRQVGGDYVESWQPHAMSAAQLERAQGVARKVTERLGGYGLFGVELFLLPND
                     QVMFSEVSPRPHDTGLVTLATQVWSEFALHARAILGLPIASIRRHAAGASVALRAEGK
                     LDSPMLEGVAEAYAIPDIDVRFFGKPNAGPRRRMGVALATADTPQAALEKAKKVAALL
                     KLRDGAASK"
BASE COUNT         1298 a         2656 c         2459 g         1349 t
ORIGIN      
        1 cctctcgcag atttgcggcg cactgcacga ggcgcacgga cgcggcgttc gctgtcgcga
       61 tatcaagcct gcgaacgtga tgctctgccg gcgtggcggc atccctgact tcgtcaaagt
      121 gctgagcttc ggcctcacgg ccgaccacac gagcgacgca gcgcccgtcg tcgaaggtgt
      181 cgcaatgctc gcgaagttct tgctcggcac cgatcgcgtg attccacccg cgctctccgc
      241 gctcgtcgac gattgcctcg caggaaggct cacgtccgcg agcgagctgg cggatcgcct
      301 cgcagcgctc gagctcggcg cgacgtggac ggacgaacaa gcgaacgcgt ggtggtcaac
      361 ccacaacggc gcgagctcgc cttcgggccg agcctcgact ccgggacttc cgcaacacgt
      421 cctcgagatc gatctcgcag agcaccgcgc gtagccgcta cttgccgatg cgggacgagc
      481 gaacagcgtc ccaccacgga gccgagtact tcggcggtgc ctggccgttc ggcagacgcg
      541 ggccgggcgg ttggatcggg aggacggcaa cgccgccggt gacagtttcg agggactggt
      601 tgtcgagctt ggtcatggca gctcgtagag cagcctgcgt accagtggaa gcgtgagcta
      661 agcgcgcgag atcgatggct gactggtcga gttgccgtcg cgcggggatg gcggagtgtt
      721 aaccgcggag cgcagcgttc gagctagcga ggacacgaga aattcgccgc ggtcggagtc
      781 accggttgcg gcgagccgat cggtccccac gtgaagctgc cgatgcagcg caggccggcc
      841 gtaccattca tgcatgtatg agtgcgcccg agcgtgagct tcgtcgcgcc gtcgaacagt
      901 ggagcgcgca ccggcgagag gtgcgtcgtc gtcgtgccgt cgccgagctg tcccgcgctg
      961 ttgtcgcccc agcatgacca ttccgcgagg ttgttcacgg cgcagacgtg gtgcggtccg
     1021 gcggcaaacg cggtgacgcc gctgagtccc acgagcgtcg gattcgcgtt cttcgtcgtg
     1081 tcgatcccga tctgtcctcg acggttgtcg ccccagcaat acatgccgct cgccgcgagc
     1141 gcgcacgtga agagcccgcc ggcctgaatg ttcgaaatgt tggcgatgcc gatcatgtaa
     1201 ggcgtgtacg cgtccgtggt ccgatccggc tcgccgatct ggagctcgtt gccacgaccc
     1261 gcgcagtacg cagccgccgt cgccagccca caggtatgcg cggcgccggc gtcgacgtaa
     1321 ctgagcgctg gaaagttcga attcgtcggt gggagcggcc ccatcatgtt gttcggcagc
     1381 gccgattggc cctcgttgtt cgcgccccaa caccacgtcg tcgagtcgga aaggaccgcg
     1441 catgtcgacg acgttgcggg cgcgatgtag ttcacgttct gcggcagcga gatggcgagc
     1501 ggtgttgtct gcggatgcgc ggcacccatc gccacgttct ccgcatcgtt atcgccccaa
     1561 caccacgcgg tgcggtcgga tcgaatcgcg catgcgcttg cattgccggc ctcgacattg
     1621 atcacctgat cgatcatcgc gggcgctggc gtcgagtgcg cgatcttcgt gccgtcaccg
     1681 agctgtcccg acgagttcac gccccagcag cgcagcggtt gtcccgcgac gccagccggc
     1741 acggcgcacg tgaagtcttc accactggcg aattgcgacg cgttcatgcc ggtctcgaac
     1801 agcgctgtgc gatcgatcgt cgcgtcgtcg cctcgttccc ccacgcggtc gctgccccaa
     1861 caccaaagcg ttccttgcgt cgagatcgcg cacgcgtgcg agacaccgac ggagatcgct
     1921 tgcgcgaacg gaactgcgac ctggcgaggc acgtcgctcg atcgcaagtc ggcgttgcct
     1981 gcttggccgt cgctgttcgc gccccagcac cacaccgtgc ggtcgctctt gcgcacgcag
     2041 aacgtgtcgg ccttcgcagc gatctcgacg gcgtcggcga tgctcgcttt cactggtatc
     2101 aggctatcgg tgcgcgtgcc gttgccgagc tgccccgtgt cgttgatgcc ccagcagtag
     2161 acatcgccac cggcgacgat cgcgcacgac gctttgctcg cgagcgcgac atggatcgcc
     2221 gtcgagagac cgaccgtctg gacgggcacc atcgtgagcg tcgtcgtgtt gtcaccgagc
     2281 tggcccttgt cgttggtgcc ccaacaccac acggttccgt cgcgtccgac ggcgcaagcg
     2341 tgcgtcgcgg acaacaccgc gtcgagcgac gacaacgcag cgtcgataat cggcggcaca
     2401 ccgagcacgt gcacgggctg attgctctgg gtattgacgc ctggatagcg gccccagcac
     2461 cacagctcgc cggcgtccgt ccacacgcag gtgttcatgc cgcccgctgc gacgcctgcg
     2521 atcgacgccg gtgcgttcga cactgcagtg ggcgacgtct gctgcacgag attgccgatg
     2581 ccgagctgtc ccgattcgtt gcgaccccag caatacagcg cgttgccgac gacggcgcac
     2641 gcatgcgcag ctccagtcgc gaacgcgatc acgttcccga gcggcaagaa atacggatcg
     2701 attcggctcg tgcgcgtgcc gtcgcccatc tggccttcgc tgccgtcgcc ccagcagcgc
     2761 aagccgcccg gcgcattcac gcacgtgtag ttgccgccgg cgccaacacc cagtgcggtc
     2821 gtgacgggcg tgcagtcgtc gacaggtggg agcgtgtcgc caggatcgag cagatcgaag
     2881 ccgatccgtc cgcaacctcc gacgatcacg gcgatcgtca gccagtagca acgcatcgac
     2941 gcgatcgtac ccgagcctgc ctcctcacgc gttgttcacg cgctgaatca ggcgacttca
     3001 cgcgcgaccc gcgcaatgtc gacctcaaga aaggacattg ctcatgaaga ccctcctcgc
     3061 cgccacactc ctcgccgctc tctccgccac cgcgtttgca cagggggccg acccgcaggc
     3121 tccgcaacca gcacccgacg cgcagcccgc agtcggcgac tccacgacga cgcagccgtg
     3181 ctcgcaagcg ctgcagcaac cgccgtgtgc gacacccacc gtcgcgtatg cagagccggc
     3241 accactgacg ccgcaacccg tgctcgacac accgcaggtc gatgacgacg gcggctggca
     3301 caagcccgtc ggacgtcgtt tcctccacgg cttccgcctc ggttggacgt atctctacaa
     3361 cgtcgaaaag atgcgactcg actcgaaggg catgcccacc gtcgagagcc tcaaggacaa
     3421 gtacggcatc aagtcagcca acatgatgct cctcgggtac gagggcttct accgcatcat
     3481 cggtcactcg tggctcaacg tgctcatggt cggcaacgtc accgtcgcgg gcctcgagca
     3541 gagcaagttc atccccgctg caagtggcct catcggcttc gagttcaacc gcagctttca
     3601 gctcggcgtc ggcgtgaacc tgacgcccga tccgcatgcg ccgtcgcaca tgatcgcagc
     3661 cgcgggctgg acgccgaacg tcggcagcat ccagatcccg ctccacttct tcttcgtgcc
     3721 cgacaccgat agcaaccacc gcatgggcgc gacgatcggt atgacttggt agctagccga
     3781 gctctttgac gcgagcttcg agtgcggtcg taagcgccgc ttcgagctcg tcgctgacgt
     3841 tctcgttgcg aagctgttgg atggaggtct gtaggagggc cttcttggcg gcggggctcg
     3901 acgtgcgttg cagcttgtcg agcatcggcg cgagctcgag tcgggctacg gtctggacga
     3961 gctcgcgccg aggttggggt tggtcgatca tctcgagtgc gcggccgagc gcgacgcgtc
     4021 gtgcgacttt gccgtcggtg ctctcgatgc gagcgatcca atcgcgcgcg gtgagcggcg
     4081 gaggtgcaac accagccgcg acggcgggcg tcggaacggc gagacgcgcc ttcttgtagc
     4141 catcgaggag cttgcgcagc tgcggcgtct cgtttgcgag aatggcagcg ttctgctcgg
     4201 cgaagcgcgc cgcgaatgtc ggcgactcga acgtgaacga gtgcgtcgtc ccattccagc
     4261 cgccatatgc gagcgcgacc tgcggcgatg cgcaggaagc tccgcacgcg tgcttggcgg
     4321 aggcctttcc cgacgagcgc agatacgctg ctgccgcgat tgcgatgacg agcacgatcg
     4381 cagcaacgag gaacgacacg acgagcgcga gcacgatcgc gaggaccagc ccgccgaccg
     4441 ccattcccat cgcgcggata ccggcccact cccacttgtc gacgtggccg acgcacctct
     4501 tgcaatacgg cgtatcgagg gcgtggccgg tctccgacgt cacgcgcatc tccgcatcgg
     4561 gtgccgcgcc acaacacggg cactcgtacg gcacgccgta cttccgagac gagacctcca
     4621 cgatcacgcc acgcatcgag atcgagtcta tccgaaacgg attccctccg gtcacacgag
     4681 gtgggcggcc atgtccttgc gctgggcctc gtctggaatg cgcccgaccg ccgccagcac
     4741 gttatcggcg atgtgcttcg gattgctcgt cgcgggaatc gggcacgtca cggccggatg
     4801 cccgatgatg aacttcaaga agtactgcgc ccagctcgta caatcgatgt ctttcgcgat
     4861 cgccggcagc ggcttgccct tcacctggcg aaacagcgcg ccggactcga acggcgtcat
     4921 cacgagcacg gcggtgccgg tatcggccgc ggcgggaaga atgcgggcct ccgctttgcg
     4981 gtcgacgatg ctgtacggaa tctggatgaa gtcgagcttc tcgttcttca tcagcttctc
     5041 gatcagatcg aattggtcgt gctgatagtg cgtgacgccg aggtagcgaa tcgtgccggc
     5101 gtctttccaa tcgcggagca ccggcaggtg cgtcttgtag tcgagcaggt tgtgcacctg
     5161 catcagatcc atgcgcttcg ctcgcatgcg cttcatcgat cgctgcatgg cgtcgatgcc
     5221 ggcctgcttg ccgctcgtcc agactttcgt cgcgaggaac ggcttgccga tcgcgccgat
     5281 cgcatcgagc tcgtcgccgg tgacttcctc ggccttgcca tacatcggcg agctgtcgat
     5341 gaggcgacag cccgcgtcga ggaatgttcg cagcacgtcg tcgatcggct tgcgcacgtc
     5401 ggcgcccgca ccgacatcga atgtctgcca cgtgccgagg ccgacgaccg gcagctcttc
     5461 cttcgtgcgc ggaatcggac gcgtgtggat cgtcatcttc ttcttgtcgg cgccgtacgc
     5521 tctcgtcgag agcagcgctg cggcagcggc ggtcagctgg atgaattcgc ggcggttcac
     5581 ggacagtcga cttgcttcgc gttctcgaag catagctgct tgccgtcggg gagcacggcc
     5641 tcgcgaatgc tcgcattggc gccgaacgtg atcgtcgtgc ccttgtcgag cttcgtatct
     5701 ccgacggtga ccgatgcaaa gctgacgcag ctgacgacct ttccggcttt gtcgtagacg
     5761 acgcgaccgg ggccgcacgc aaccacttgc tccttcggcg ttcccgccgg cgtctcggga
     5821 ttcgcgaaca tcatctcggt ctcgcacacg aggctgccgt catcctgcgc gacgtccgtg
     5881 ccggccttgc acatcgcggg cacgccgttc ggaccggcgg gcgccgccga tccagcggcc
     5941 actgcgatcg ggtccggggc gtcgagcttc ggctcgggaa tcttcacgtc cggaagcggc
     6001 ttgagcttga gcccaccgct cgcgtcgagc agcgtctgca gcttgcggtc ccagctcatg
     6061 acggattcct gcagatccat catctgcgca tgcgcctctt tgaacttcgc cgagtcgaca
     6121 gggtcagtcg ccgcagccat cgcgctgatg atgtcgctct cgcgcttcca caagccgagc
     6181 gtgtcctcgt gcagctgctt ggccgcgggg ggcggcgtga ggacgctcgc tccggccact
     6241 gcctccttca agaacggggc cgcgacatcg cgaaacagcc acttcatctt ctcgggctgc
     6301 tcgtcgatat cgacggcgcg cacgctgcca tacgccgacc gcgcagccac gaccttcgcc
     6361 agctgcggcg ccaaatcgac gttcacgtag gcatccacca cctgcgaatc ctcctttttc
     6421 ttcttcccac cgcaccccgc gagaacgacg acaacgagcg cgatcacgag tgacttcatg
     6481 ccgcgcatct tacgtcagac gcatctgcta ttccggaccc atgaaacccg tctcgttcgg
     6541 cactcccaag agcccacacg ccacccgcgt cctcctcctc ggatcgggag agctcggcaa
     6601 ggaagtcgtc atcgagctcc agcggttcgg cgtggaagtc atcgcgtgcg atcgctacgc
     6661 cgatgctccc gcgatgcagg tcgcgcaccg ctcgcacgtg atcgacatgc tcgatcgcgc
     6721 cgcgctccgc gccgtcatcg agcaggagaa gccgcacctg atcgtccccg aaatcgaagc
     6781 catcgcgacc gcggagctcg tcgagctcga gcgcgaaggc tggaatgtca tccccaccgc
     6841 gcaagccgcg cgcctcacga tggaccgcga agcgatccgc cgcgtcgccg ccgaagagct
     6901 gaagctgccc actgcccgct accaattcgc ggacaccttc gaagagctgc aatccgcgtc
     6961 gcgcgccctc ggctggccgg tcgtcatcaa accgatcatg tcctcctcgg gcaaaggcca
     7021 gtcgctcgca cacaccgaag ccgatctcga aaagagctgg cactacgcac aaacgggcgg
     7081 gcgcacaggc gcaggccgcg tcatcgtcga agagttcatc aagttcgaca gcgagatcac
     7141 gatgctcacg gtgcgcgccg ccaacggcac cttcttctgc gaccccgtcg gccaccgcca
     7201 agtcggcggc gactacgtcg agtcgtggca gccgcacgcg atgtccgccg cccagctcga
     7261 acgcgcacaa ggtgtcgcgc gcaaggtcac cgagcgcctc ggcggatacg gcctgttcgg
     7321 cgtcgagctg ttcctgctgc ccaacgacca agtaatgttc tcggaagtca gcccgcggcc
     7381 gcacgatacg ggcctcgtca ccctcgcgac gcaagtctgg tcggagttcg cgctgcacgc
     7441 gcgcgcgatc ctaggcctcc cgatcgcctc gattcgtcgc cacgccgcgg gcgcgtcggt
     7501 cgcgctgcga gcggaaggca agctcgactc gccgatgctc gagggtgtcg cggaggcgta
     7561 cgcgattccc gacatcgacg tgcggttctt cggcaagccg aatgcgggac cgcgccgccg
     7621 gatgggtgtg gcgctcgcga cggccgacac gccgcaggcg gcgctcgaga aggcgaagaa
     7681 ggtagcggcg ctgctgaagc tgcgcgacgg cgcggcgtcg aagtaacagc cgctgcagca
     7741 accgcaaccg cagccgacgt gc
//
LOCUS       DATLBZ010000382         2896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1939537, whole genome shotgun sequence.
ACCESSION   DATLBZ010000382 DATLBZ010000000
VERSION     DATLBZ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939537"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VMZ53_19575"
     CDS             <1..1035
                     /locus_tag="VMZ53_19575"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HUS30724.1"
                     /translation="TLQSIVMDAPHADDVTVEKLLAALAPHTQLVIAVGTGTLNDLAK
                     MVAFRHGCPSAVFATAPSMNGYTSLSASITTNGVKTSFRTRTPLGVYFDLRVLAAAPP
                     RLIRAGLGDSACRSTAQADWLLSHLLLDTPYREAPFALLAHDERDVLANTSALLAGDL
                     EVMRHLVRTLVLSGFGMTICNGSYPASQGEHLLAHYITMMGRDIPPSFHGEQVAVCTV
                     AMAAIQERILARDTAPVLRPPTATRSSIIEHFGLTMGPMCWADFEHKQLDAAALNARL
                     ARDWDTIRARITSVTVPHAKLRASLVDAGAPVEHSQLGWSDELWSNALHHAREIRNRY
                     TFLDVANDSD"
     gene            complement(1032..1817)
                     /locus_tag="VMZ53_19580"
     CDS             complement(1032..1817)
                     /locus_tag="VMZ53_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HUS30725.1"
                     /translation="MSTLDLTLHIWRQRNRETAGAFKTYKVTGVSTHASFLEMLDILN
                     EQLTAKGEEPVAFDHDCREGICGMCSLMINGRAHGGRRATTTCQLHMRFFNNNDEIWI
                     EPWRAAAFPVIKDLCVDRTPFDRIIAAGGFITANAGSAPDANDIPVPTEAADRAMDAA
                     ACIACGACVAACPNGSAMLYVAAKLAHLHNLPQGIPEQMHRTKAMIGAMDAAGFGACS
                     NHYECEAVCPKEIPRDVMAKTYRDFAKASFAHRERESKSDGAG"
     gene            complement(1814..>2896)
                     /locus_tag="VMZ53_19585"
     CDS             complement(1814..>2896)
                     /locus_tag="VMZ53_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HUS30726.1"
                     /translation="QSKLTLMSESLRNDGRVWVPKRADDVSKHPDKIPDEDRDYYLER
                     KYPSFGNLSPRDIASRAAKEVCDEGRGVGPGGRGVYLDFRDAIKRDSAAKIAEKYGNL
                     FDMYERITGEDPYKVPMRIYPAVHYTMGGLWVDYHLQTTIPGLFCLGEANFSDHGANR
                     LGASALMQGLADGYFIIPYTIGHYLAGVPSASLADPTSAAFTTIEAEMRARLDKLMSG
                     TGKRSIDSLWKELGHIMWEGCGMARSKASLEHALAEIPRLREQFWSDVSVLGERDSFN
                     QSLEKAGRVADFMEFAELMCRDALEREESCGGHFRVEHQVEGEAKRDDDRFCHVAVWE
                     HAAAGPIRHTEQLTFENVKLATRSYK"
BASE COUNT          448 a          941 c          980 g          527 t
ORIGIN      
        1 acgctgcagt cgatcgtgat ggacgcgccg cacgccgatg acgtcaccgt cgagaagctg
       61 ctcgccgcgc tcgcgccgca cacgcagctc gtcatcgctg tcggcacggg cacgctcaac
      121 gacctcgcga agatggtcgc cttccgtcac ggttgcccat ccgcggtgtt cgcgacggcg
      181 ccgtcgatga acggctacac gtcgctgtcc gcgtcgatca cgacgaacgg cgtgaagacg
      241 tcgtttcgca cgcgcacgcc gctcggcgtg tacttcgact tgcgcgtgct tgccgccgca
      301 ccaccgcgcc tcattcgtgc aggcctcggc gacagcgcgt gtcgctcgac ggcacaggcc
      361 gactggctgc tctcgcacct gctgctcgat acgccgtacc gcgaggcccc gttcgcactg
      421 ctcgcgcacg atgaacgcga cgtgctcgcc aacacgagcg cactgttggc gggcgatctc
      481 gaggtgatgc gccacttggt ccgcacgctc gtcctcagcg gattcggcat gacgatctgc
      541 aacggcagct atccggcgag ccaaggcgag cacctgctcg cgcactacat caccatgatg
      601 ggccgcgaca tcccgccgag cttccacggc gagcaggtcg cggtctgtac cgtggcgatg
      661 gcggcgatcc aagagcgcat cctcgcgcgg gataccgcgc cggtgctgcg tccgccgacg
      721 gcgacccgct cgtcgattat cgagcacttc ggcctgacga tgggccccat gtgctgggcc
      781 gacttcgagc acaagcagct cgacgcagcc gcgctcaacg ctcgactggc gcgcgactgg
      841 gacacgatcc gtgcgcgcat cacctcggtg acggtgccgc acgccaagct tcgagcctcg
      901 ctcgtcgatg cgggggcgcc ggtcgagcac tcacagcttg gctggtccga cgagctgtgg
      961 agcaatgcgc tccaccacgc gcgcgagatc cgcaaccgct acacgttcct cgacgtcgcg
     1021 aacgacagcg actagccggc gccgtcggac ttggactcgc gctcgcggtg cgcgaacgac
     1081 gccttcgcga agtcgcggta tgtctttgcc atcacgtcgc gcggaatttc tttcgggcag
     1141 acggcctcgc actcgtagtg gttcgagcat gcgccgaagc cggcggcgtc catcgcgccg
     1201 atcatggctt tcgtgcggtg catctgctcg ggaatgccct gcggcaggtt gtgcaggtgc
     1261 gcgagcttgg ccgcgacgta cagcatcgcc gagccgttcg ggcacgcagc gacgcacgca
     1321 ccgcacgcga tgcaggcggc ggcatccatt gcgcggtcgg cggcttccgt ggggacggga
     1381 atgtcgttcg cgtcgggagc ggagcccgcg ttcgccgtga tgaagccgcc tgcggcgatg
     1441 atgcgatcga acggcgtgcg atcgacgcag agatctttaa tcaccgggaa cgcggcggcg
     1501 cgccacggct cgatccagat ctcgtcgttg ttgttgaaga accgcatgtg cagctggcag
     1561 gtggtggttg cgcggcgtcc gccgtgcgcg cggccgttga tcatcagcga gcacatgccg
     1621 cagatgcctt cacggcagtc gtgatcgaat gcgacgggct cttcgccttt cgcggtgagc
     1681 tgctcgttga ggatgtcgag catttcgagg aacgaggcgt gcgtggagac gcccgtcacc
     1741 ttgtaggtct tgaacgcgcc ggcggtttcg cgattgcgct ggcgccagat gtggagcgtc
     1801 agatcgagcg tgctcatttg tagctccggg tcgcgagctt cacgttctcg aacgtgagct
     1861 gctccgtgtg gcggatagga ccagcggcgg cgtgctccca cacggcgacg tgacagaacc
     1921 gatcgtcgtc gcgcttcgct tcgccttcga cttggtgctc gacgcggaag tggccgccgc
     1981 acgactcttc gcgctcgagc gcgtcgcggc acatcagctc ggcgaactcc atgaagtcgg
     2041 cgacgcgtcc ggctttctcg agcgactggt tgaacgagtc gcgctctccc agcacggaga
     2101 cgtcggacca gaactgctcg cgcaaacgcg ggatctcggc aagggcgtgc tcgagcgacg
     2161 ccttgctgcg cgccatgccg cagccttccc acatgatgtg gccgagctct ttccacagcg
     2221 aatcgatcga gcgcttgccg gtgcccgaca tcagcttgtc gaggcgcgcg cgcatctcgg
     2281 cttcgatcgt cgtgaacgcg gcggacgttg gatcggcgag cgacgcggac ggcacaccgg
     2341 cgaggtagtg gccgatcgtg tatggaatga tgaagtagcc gtcggcgaga ccttgcatca
     2401 gtgcgctcgc gccgaggcgg ttggcgccgt ggtcggagaa gttggcctcg ccgaggcaga
     2461 acaggccggg gatggtcgtt tgcaggtggt agtcgaccca gagcccgccc atcgtgtagt
     2521 ggacggcggg gtagatgcgc atcggcacct tgtacggatc ttcgccggtg atccgctcgt
     2581 acatgtcgaa caggtttccg tacttctcgg cgatcttggc ggcgctgtcg cgcttgatcg
     2641 cgtcgcggaa atcgagatag acgccgcggc caccggggcc gacgccacgg ccttcgtcgc
     2701 agacttcttt ggcggcgcgc gatgcgatgt cgcgaggcga gaggttgccg aagctcggat
     2761 actttcgctc gaggtagtag tcgcgatctt cgtcggggat tttgtcggga tgcttgctga
     2821 cgtcgtccgc gcgcttgggc acccagacgc ggccgtcgtt gcgcagcgac tccgacatca
     2881 gcgtcagctt cgattg
//
LOCUS       DATLBZ010000383         3636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9389513, whole genome shotgun sequence.
ACCESSION   DATLBZ010000383 DATLBZ010000000
VERSION     DATLBZ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9389513"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VMZ53_19590"
     CDS             <1..241
                     /locus_tag="VMZ53_19590"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HUS30727.1"
                     /translation="IRMRSRDVESQIPLGYLQALHAGYQEFIANISRTIPVIRIDYER
                     FATAEEMAEVIKREYLDASFLRQVTRFDPATGVSP"
     gene            complement(288..980)
                     /locus_tag="VMZ53_19595"
     CDS             complement(288..980)
                     /locus_tag="VMZ53_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30728.1"
                     /translation="MAAGLAATTAVGAWMVVHRADDAGAPQLVAPAIDEEPAATIAKD
                     DAPPEAPPVVVDEPGDATWMPPGYVENISRDKSEELAAFVQEWITQGNAPGPAIEYKR
                     GIVFAESEEDRGDDGPYPKSAAAEGKRVCGSEAVWLREYIRERLKLGVEMGGISCAGN
                     VCSYDGMEYAPNGYLVFKEIDDRWTLIAWTQVYSAALSQEAADANIRSVIANMIKLRD
                     TRCVGEPAGTYY"
     gene            complement(1054..2334)
                     /gene="eno"
                     /locus_tag="VMZ53_19600"
     CDS             complement(1054..2334)
                     /gene="eno"
                     /locus_tag="VMZ53_19600"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HUS30729.1"
                     /translation="MTQIVWLHAREVLDSRGNPTVEAEVELDNGAHGTAIVPSGASTG
                     EHEALELRDGDAKRYLGKGVTKAVNNIRDVIAPAIIGMDASNQAEIDGAMIDLDGTAV
                     KSNLGANAILAVSMAVARAAANAHELPLYRYLGGVGAVTLPVPLMNVINGGAHADNNL
                     DIQEFMIVPAGFDRFEDALRAGVETFHNLKKLLSSRGKATNVGDEGGFAPNIDSGDEA
                     LSLLMEAISKAGYEPGKQIFIALDVAASELFDKKNGTYTWQSQQRKAGDLVNIYAEWA
                     EKYPLVSVEDGLAEDDWDGWKALTTKLDGKIQLVGDDLFVTQTARLKRGIEGGIANSI
                     LVKVNQIGSLTETLDAVRTAQHSGYTAIISHRSGESEDSFIADLAVATNAGQIKTGSA
                     SRSDRIAKYNQLLRICDQLGDEARFAGPTVFKRS"
     gene            complement(2345..2527)
                     /locus_tag="VMZ53_19605"
     CDS             complement(2345..2527)
                     /locus_tag="VMZ53_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30730.1"
                     /translation="MRERSLGDPREPGHSFCMALETYPNDEKQASLAIILLSIMSVII
                     VGLITAAVLSTRACSV"
     gene            2550..3029
                     /locus_tag="VMZ53_19610"
     CDS             2550..3029
                     /locus_tag="VMZ53_19610"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS30731.1"
                     /translation="MKHAAIFFVALALAGCPRGSAPTPTHPSTPKETVAGARATVEQW
                     RQAYQVKSFDALAKLYVQSLELVVVQDGSPLVGWTSVEGMLKDRFARYKDVQIRLKDI
                     QVVALDGTSAVATASMTRELGDGVTTITESGALTVVLRKEGDLWLIVAEHYSYKRST"
     gene            3029..>3636
                     /locus_tag="VMZ53_19615"
     CDS             3029..>3636
                     /locus_tag="VMZ53_19615"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HUS30732.1"
                     /translation="MSPAGEASRTRTIGVALLGLGNVGGGVVKLLADNAEAIQARLGA
                     RIEVRSIAVRDPDKVNRVVEVDKKLLTTDMDAAIARPDVDVVCELIGGTTDAKRAVLR
                     AIGAGKQVVTANKALLAEHGAEVFGAAEQSGVDVYYEAAVCGGVPIIRVLREGLASDR
                     VEALWGIVNGTSNYILSTMTETRRAFGDILADAQRLGYAEADP"
BASE COUNT          598 a         1169 c         1215 g          654 t
ORIGIN      
        1 catccgcatg cggtctcgcg atgtcgaatc gcaaatcccg ctcggctacc tgcaggcgct
       61 gcacgccggc taccaggaat tcatcgccaa catctctcgg acgattccgg tgatccggat
      121 cgactacgaa cgcttcgcga ccgccgaaga aatggccgaa gtgatcaagc gcgagtacct
      181 cgatgcgtcg ttcctccgcc aagtcacgcg cttcgatcca gcgacgggcg tctcgcccta
      241 acgaacgact ccgccggcgt agaaggcgag atcgacttcg ccggccgtca gtagtacgtg
      301 cccgcgggct cgccgacgca gcgcgtgtcg cgcagcttga tcatgttcgc gatcacgctg
      361 cggatgttcg catcggcggc ttcctgcgag agcgcggccg agtagacctg cgtccacgcg
      421 atgagcgtcc accggtcgtc gatctccttg aagacgagat agccgttcgg cgcgtactcc
      481 atgccgtcgt acgagcacac gttgccggcg cacgaaatgc cgcccatctc gacaccgagc
      541 ttcaggcgtt cgcgaatgta ctcgcgcagc cagacggcct cgctaccaca gacgcgcttg
      601 ccttcggccg cggccgactt gggatacgga ccgtcgtcgc cgcgatcttc ctcgctctcg
      661 gcgaacacga tgccgcgctt gtactcgatg gctgggccgg gcgcgttgcc ctgcgtgatc
      721 cactcttgga cgaacgccgc gagctcctcg ctcttgtcgc gcgagatgtt ctcgacgtaa
      781 cccggcggca tccacgtcgc gtcaccgggc tcgtcgacaa cgacaggcgg cgcttccggt
      841 ggcgcgtcgt ccttcgcgat cgtcgcggcg ggctcctcgt cgatcgccgg tgccacgagc
      901 tgcggcgcgc cagcatcgtc ggcccgatgc acgaccatcc acgcgccgac cgcggtcgtc
      961 gccgcgaggc cagctgccac cgaaacacgc gccaggatct gcatgccgat ctaacgcgga
     1021 gcagcaggcg cgtattcgcg cgtgaaccgg ctgttacgag cgcttgaaga cggtcgggcc
     1081 cgcgaagcgc gcttcgtcgc cgagctggtc gcagatacgg agcagctggt tgtacttggc
     1141 gatgcggtcg gagcgcgagg cggagccggt cttgatctgg cccgcgttcg tcgcgacggc
     1201 gaggtcggcg atgaagctgt cctccgactc gcccgagcgg tgcgagatga tcgcggtgta
     1261 gccgctgtgc tgcgcggtgc ggacggcgtc gagcgtctcg gtcagcgagc cgatttggtt
     1321 caccttgacg aggatcgagt tcgcgatgcc gccctcgatg ccgcgcttga ggcgggctgt
     1381 ctgcgtgacg aacaggtcat caccgacgag ctggatcttg ccgtcgagct ttgtcgtcag
     1441 cgccttccag ccatcccagt cgtcttcggc gaggccgtct tcgacggaga cgagcgggta
     1501 cttctcggcc cactcggcgt agatgttgac gaggtcgccg gccttgcgct gctgcgactg
     1561 ccacgtgtac gtgccgttct tcttgtcgaa cagctcgctc gccgcgacgt cgagcgcgat
     1621 gaagatctgc ttgcccggct cgtagcccgc tttgctgatc gcttccatga ggagcgagag
     1681 tgcttcgtcg cccgagtcga tgttcggtgc gaagccgcct tcatcgccga cgttcgtcgc
     1741 cttgccgcgc gaggagagca gcttcttgag gttgtggaac gtctcgacgc cagcgcgcag
     1801 cgcatcttcg aagcgatcga agcccgcggg cacgatcatg aactcttgaa tgtcgaggtt
     1861 gttgtcggcg tgcgcgccgc cgttgatgac gttcatcagc gggacgggca gggtgaccgc
     1921 accgacgcca ccgaggtagc gatagagcgg cagctcgtgg gcgtttgctg ctgcacgcgc
     1981 gacggccatc gacaccgcga ggatcgcgtt cgcgccgagg ttcgacttta cggccgtacc
     2041 gtcgaggtcg atcatcgcgc cgtcgatctc ggcttggttc gaggcatcca tgccgatgat
     2101 cgcgggtgcg atcacgtcgc ggatgttgtt gaccgctttc gtgacgcctt tgccgagata
     2161 gcgcttcgcg tcgccatcac ggagctcgag tgcttcgtgc tcgccggtcg acgcgccaga
     2221 gggaacgatg gccgttccat gcgcgccgtt gtcgagctcg acttcggctt cgacggtcgg
     2281 gttgccacgg gaatctagaa cttcacgagc gtgcagccag acgatctgcg tcatgcgccg
     2341 gacactacac cgagcatgcg cgagtggaca gcaccgcagc ggtgatcaag ccgacgatga
     2401 tgacggacat gatcgagagc aagatgatcg cgagcgaagc ctgcttttcg tcgttaggat
     2461 aggtttcaag cgccatacag aaactgtggc caggctctcg cgggtcgcca agcgagcgct
     2521 cccgcacggt cgcgtgatat gacgcgcgca tgaagcacgc tgcgattttc tttgtcgcgc
     2581 tcgcgctggc tggctgcccg cgcggcagtg cgccgacgcc gacgcatccg tcgacgccga
     2641 aggaaacggt cgccggcgcg cgcgccacgg tcgagcagtg gcgtcaggcg tatcaggtga
     2701 agagcttcga cgcgctcgcc aagctgtacg tgcaatcgct cgagctcgtc gtcgtgcagg
     2761 acgggagccc gctcgtcggt tggacgtccg tcgaaggaat gctgaaagat cggttcgcgc
     2821 ggtacaagga tgtccaaatt cgcctgaagg acatccaggt cgtcgcgctc gacggcacat
     2881 cggccgtcgc tacggcatcg atgacgcgcg agctgggcga tggcgtcaca acgatcaccg
     2941 agagtggcgc gctcacggtg gtgctgcgca aagagggcga tctctggctg atcgtcgccg
     3001 agcactattc gtacaagagg agcacctgat gtcccccgcc ggggaggctt cgcgcactcg
     3061 caccatcgga gtcgcgctgt tggggcttgg caacgtcggc ggtggcgtcg tcaagctgct
     3121 cgcggataac gccgaggcga tccaagcgcg gctcggtgcg cgcatcgaag tgcgatcgat
     3181 cgcggtccgg gatccggaca aggtgaatcg cgtcgtcgaa gtcgacaaga agctgctgac
     3241 cacggacatg gacgcagcca tcgcgcgccc cgacgtcgac gtcgtgtgcg agctgatcgg
     3301 cgggaccacc gatgcgaagc gcgcagtgtt gcgtgccatc ggggcgggca agcaggtcgt
     3361 caccgcgaac aaggcgctgc tcgccgagca cggcgcggag gtgttcggcg ccgccgagca
     3421 atccggcgtc gatgtctatt acgaagctgc ggtctgcggc ggcgtgccga tcattcgcgt
     3481 cctgcgcgag ggcctcgcaa gcgatcgcgt cgaagccctg tggggcatcg tcaacgggac
     3541 gtcgaactac atcctctcga cgatgacgga gacgcgccgc gcattcggcg acatcctcgc
     3601 ggacgcccag cggctcggct acgcggaagc cgaccc
//
LOCUS       DATLBZ010000384         5955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9391314, whole genome shotgun sequence.
ACCESSION   DATLBZ010000384 DATLBZ010000000
VERSION     DATLBZ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9391314"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VMZ53_19620"
     CDS             complement(<1..881)
                     /locus_tag="VMZ53_19620"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HUS30733.1"
                     /translation="MDLGKLTPRDLIEFVTERAKHCKPGTAKLVATALRSFLRFLQMS
                     GIGDARLVAAVPIVPNWKLARLPKILTSAQLRSLLRAFDQSSATGRRDYAMVLCLAQM
                     GLRAHEVAQLALDDIDWRAGTLRIAIGKSRRESMLPLPVTVGRGLVAYLRDGRPAEAG
                     RNVFVRHRVCVGKPLTSSAVCSAIRRAFERAQIAVPSKVHMHFDTRLPRACSVVARVS
                     RISPTFFAIAASIRRRSTRRSISLVSQRWRCLGRGSHEARHDRGIGRAVSDDAPKTRL
                     RPDGRRAAADAIRSLCGS"
     gene            complement(1097..2923)
                     /locus_tag="VMZ53_19625"
     CDS             complement(1097..2923)
                     /locus_tag="VMZ53_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30734.1"
                     /translation="MGVLLVARNLAASRGLVQPSVGEILDAFDVGRSRAYEIRDVIFA
                     LLPTLVRPPGRPRLERERTPSSTLVDLLAQVHRFVTQHPGCIQSGARARYSEPYRRFV
                     LELRERHTDVMLSEFADAIQLPLGTLEDWMRTSPVDKRVVVTDAGERDAAHDAKHAQI
                     ETVLVAWRAWSGSSFAGFCNHVRRDHRIELGNTLIANILFEYGERTPARRGRLRDDEA
                     LRGSFETFFPGAQWVADGKQLEVVIDGQKFCVNLELVVDAASAAAVGISVRDEEDSAA
                     VIEAFEQGGQTTGEQPLALLLDNRPSNHTPEVDAALDDTMRIRATPFRPENKAHVEGS
                     FGLFAQKVPPLELDTREPQMLAKTLARLVATIFFRALNRAPRRDRNGKTRVDLYAQTA
                     TPEEREAARVALRERMHKQELARQTRAARLDPVVRAILDDAFARLSLLDPDRHFRDAI
                     ACYQRDVIVEAIAIFSGKRTAGTLPDGVDARYLLGIVQNVHHVHEAEAITAALIRERL
                     VASDRFLEPLVRERDAILAAGSDVGSTLITLVDRLVRAKHTVDRHVWLDAAAALVTQR
                     EHDERIALARRAARWIHATFALSTRDRDHLERSLMRRLWPLD"
     gene            complement(2978..3349)
                     /locus_tag="VMZ53_19630"
     CDS             complement(2978..3349)
                     /locus_tag="VMZ53_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30735.1"
                     /translation="MPTSEPLTAVMPLTAGLLLAAGEVVTPYGLPWPNDDEIFAATNS
                     NRAGAREIEEATGAFLPALSRSPSVGRELLLVAHASEVLRLLGMWEDFAARAGFATTA
                     NTASASNADEPPAAKHDATPA"
     gene            complement(3390..4613)
                     /locus_tag="VMZ53_19635"
     CDS             complement(3390..4613)
                     /locus_tag="VMZ53_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HUS30736.1"
                     /translation="MRKVGSTTTPVATKRLVTTAKRQAKKVLKEAKVARVAISTQKRP
                     TPSVTKSAPNEETALPKSSRKRIARSYTPSEKAVALEDAATDGVVAAAKKGGMSRFSI
                     YEWQRKVEKANAGEGPSPTSGPAPKEIEEQRDREILGEWRKHPGLGPSQIRNQLRRRG
                     IKVSVSTARHVMEDAGYRPPKVKRERHDERFEAVRPNHMWHLDFVHRHIHKASTFTLI
                     LIDDCSRFVTGHGVDDAERAEMMIRIFEEAVARHGKPESVMHDRGSAFWSWRGISRFT
                     SLLTEMGIDQVVAEKKEHNGKVEVFNANLHKELFDRHRFYDLAEMKRRLAAHLHWYNH
                     ARTHHALGGLLVPADRFYGRVEEVLARIEAGAQRDGDDLDLRERCLELFKVVSKNGAP
                     EVWLLGQRLTVPTLP"
     gene            complement(5088..>5955)
                     /locus_tag="VMZ53_19640"
     CDS             complement(5088..>5955)
                     /locus_tag="VMZ53_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30737.1"
                     /translation="NTIHAWWKNTIVGSVRLTPTRLIVRTNSIERAEALVNRVREVLG
                     SLATWKKRTREALPTMFGGETVTIDGQAMTSGSPSLHDAFRAWLDMPLAKLRGHTPRD
                     SVRDADGRRDVHLLLKEMENHHARKPVDDLDPLQLRRELGLDELGQPLPHRDLDRALG
                     AGRKLSETLLEFARPLIDSDGGPIEEHQMRSLLGFAINVWNLVVTEELHDSADDVAEV
                     RAELAPDRIPAAVLAWFDRLVVRKRERFHGDLRLVGNWRVRRRRDRMDIEMEARVPQA
                     LHAKLTTAGLIP"
BASE COUNT          988 a         1913 c         1930 g         1124 t
ORIGIN      
        1 gatccgcata gcgagcgaat cgcatcagct gctgcccttc gaccgtcagg gcgaagccga
       61 gtcttcggcg catcgtcaga tactgcgcgg ccaatgccac gatcgtggcg cgcctcatga
      121 cttcctcggc caaggcagcg ccaccgttgc gagacgaggg agatcgacct tcgcgtagat
      181 cgccgtcgta ttgaggctgc gatggcgaag aacgtcggcg agatccttga gactcgcgcc
      241 accacggagc atgcgcgtgg cagccgtgtg tcgaagtgca tgtgtacctt agaaggcacg
      301 gcgatctgcg cccgctcaaa ggctcgtcgg attgctgaac agaccgcgct gctcgtcaat
      361 ggcttcccga cgcacacccg gtgccgcacg aagacgttgc gtccagcttc cgccgggcga
      421 ccgtcacgca aataagcgac aagcccgcgc ccgaccgtca cgggcagagg cagcatgctc
      481 tcgcggcgcg actttccgat tgcgatacgc agcgtgccgg cgcgccaatc gatgtcgtcg
      541 agggcaagtt gtgccacctc gtgcgcgcga agtcccatct gcgcgaggca gagcaccatc
      601 gcgtagtcgc gacggccagt tgcgctcgat tgatcgaacg ctcgaagtaa cgaacgcagc
      661 tgggcgctcg tcaggatctt gggcagccga gctagcttcc aattcggaac gatcggcacg
      721 gcggcgacga ggcgcgcatc gccgatgccg ctcatctgga ggaagcgcag gaagcttctc
      781 agtgccgtcg ccacgagttt cgctgtaccc ggtttgcagt gcttggcacg ctcggtgaca
      841 aattcgatca gatcgcgagg agtcaacttg ccgaggtcca cgctagtcga ccgggaagcc
      901 cacttgagga actcgcgaac gtaacgggcg tacctgcggt tgtcaatcac ctaccaggcg
      961 ggaatggcgg acgcggtttc gggatgtgcg gtgtgaggca agcggttccc gaatgcggcg
     1021 acagctcaac gaccgacggt caatgccatc acgcgatcag cgcaatagga gaatgactcg
     1081 agagtcccag acgggatcaa tcgagcggcc aaagtcggcg catgagcgag cgctcgaggt
     1141 gatcgcggtc gcgggtcgag agcgcgaagg tggcgtggat ccagcgtgca gcgcgccttg
     1201 cgagcgcgat gcgctcatca tgttcacgct gcgtgacaag cgccgcggcg gcatcgagcc
     1261 agacgtgtcg gtcgacagtg tgcttcgcac gcacgagtcg atcgacgagc gtgatgagcg
     1321 tcgatccgac gtcgctgcct gcggcgagga tcgcatcgcg ttcgcggaca aggggctcga
     1381 ggaatcgatc gctcgctacg aggcgttcac ggatcagcgc tgcggtgatg gcttcggcct
     1441 cgtggacgtg atggacgttc tggacgatgc cgagcagata tcgcgcgtcg acaccatcgg
     1501 gtagcgtgcc ggccgtgcgc ttaccggaaa agattgcgat cgcttcgacg atgacgtcgc
     1561 gttggtagca agcgatcgca tcacggaaat gacgatcggg atcgagaaga ctgaggcgcg
     1621 cgaacgcatc atcaagaatc gcgcggacta cgggatcgag ccgtgcggca cgcgtctgtc
     1681 gcgcgagctc ttgcttgtgc atgcgttcgc gcagcgcgac gcgcgccgcc tcgcgctctt
     1741 cgggcgtggc cgtctgcgcg tacaggtcaa cccgcgtctt gccgttgcgg tcacgacgag
     1801 gagcgcggtt gagtgcacgg aaaaagatcg tcgcgacgag ccgagcgagc gtcttcgcga
     1861 gcatttgtgg ctcgcgcgta tcgagctcga gcggcggcac cttttgggcg aagaggccga
     1921 agctgccctc gacgtgcgcc ttgttttccg gacgaaacgg cgtcgcgcgg atgcgcatcg
     1981 tgtcgtcgag ggcggcatcg acctcggggg tgtggttcga cggacgattg tcgagcagca
     2041 gcgcgagtgg ttgctcgccg gttgtttgtc cgccctgttc gaatgcttcg atcaccgcgg
     2101 cgctgtcctc ttcatcacgc acgctgatcc cgaccgcggc ggcgctcgct gcatcgacga
     2161 cgagttcgag gttcacgcag aatttctggc cgtcgatcac gacttcgagc tgcttgccat
     2221 cggcgaccca ctgcgcaccg gggaagaagg tctcgaagct gccgcggagt gcttcgtcat
     2281 cgcgcaaccg accgcgtcgt gcgggcgtgc gttcaccgta ctcgaacaag atgtttgcga
     2341 tgagcgtatt gccgagctcg atccggtggt cgcgccgcac gtgattgcag aagccggcga
     2401 aggaggatcc gctccatgca cgccacgcga cgaggacggt ctcgatctgt gcatgcttcg
     2461 cgtcgtgcgc cgcgtcgcgt tcgcctgcgt cggtgacgac cacgcgcttg tcgacgggtg
     2521 aagtccgcat ccagtcttct aacgtgccga gcggcagctg gatcgcgtcg gcgaattccg
     2581 acagcatcac gtcggtgtgt cgctcgcgca gctcaagcac gaagcgccgg tacggctccg
     2641 agtaacgggc gcgtgcgccg ctctgaatgc atcccgggtg ctgcgtcacg aagcggtgca
     2701 cttgggcgag gaggtcgacg agcgtcgagg atggcgtgcg ttcgcgctcg agacgtggtc
     2761 ggcccggcgg tcgcacgagc gtcggcagga gcgcgaagat cacgtccctg atttcgtacg
     2821 cgcgcgagcg accgacgtca aacgcgtcga ggatctcgcc gacgctgggc tgcacgaggc
     2881 cacgcgatgc ggccaggttg cgcgccacca gcagcacacc cacggttacg agcggcgtga
     2941 tggaggatgg atcggggatc gacagagcgg tctcgatcta cgctggcgtc gcgtcgtgct
     3001 tcgccgcggg tggctcgtcg gcattcgacg cgctcgcggt gttcgcggtt gtcgcgaagc
     3061 cagcgcgcgc ggcgaaatcc tcccacatgc cgagtaagcg aagcacctcg ctcgcatggg
     3121 cgacgagcag gagctcgcga ccaacactcg gcgagcgcga gagcgccggc agaaacgcgc
     3181 cggtcgcttc ctcgatctcg cgggcgccgg cgcgattcga gttcgtcgcc gcgaagatct
     3241 cgtcgtcgtt cggccatggc aagccgtacg gcgtgaccac ctcgcccgca gctaacagca
     3301 ggccggcggt cagtggcatg acggcggtaa gcggttcaga ggtcggcacg ggctgtttat
     3361 gcccgagacg tctgacaacg ccttcgcgtc tacgggagtg tcggcaccgt caggcgttgc
     3421 ccgagcagcc acacctcggg agcgccgttc ttgctcacga ccttgaacag ctcaaggcat
     3481 cgttcgcgta gatcgagatc gtcgccgtcg cgttgtgcgc cggcctcgat tcgcgcgagc
     3541 acttcctcga cgcgaccgta gaaccgatcc gccggcacca gcagcccgcc gagcgcgtga
     3601 tgcgtacgcg catggttgta ccaatgaagg tgagccgcga gtcgtcgctt catctcggcg
     3661 aggtcgtaga accggtgtcg atcgaacagt tccttgtgca ggttcgcgtt gaacacctcg
     3721 accttgccgt tgtgctcctt cttctcagca accacctggt cgatacccat ctcggtcagc
     3781 agcgacgtga accgcgagat tccgcgccac gaccagaacg ccgaaccgcg atcgtgcatc
     3841 acgctctcgg gtttgccatg acgtgccacc gcctcctcga agatgcggat catcatctcg
     3901 gcgcgctcgg cgtcgtcgac gccatgaccg gtgacaaacc gcgagcagtc gtcgatcagg
     3961 atcaacgtaa acgtcgacgc tttgtggatg tgccggtgca cgaagtccag gtgccacatg
     4021 tgattcggcc gcaccgcctc gaagcgctca tcgtgtcgtt ctcgcttcac cttcggcggc
     4081 cgatagccgg cgtcctccat cacgtgccgc gcggtcgaga ccgacacctt gatgccgcga
     4141 cgacgaagct gattccggat ctggctcggt cccagccctg ggtgcttcct ccattcgccg
     4201 aggatctccc gatcgcgttg ctcctcgatc tccttcggcg ctgggccgct cgtcggcgac
     4261 ggtccctcgc cggcgttggc cttctcgacc ttgcgctgcc actcgtagat cgagaaccga
     4321 ctcatcccgc ccttcttcgc tgctgcgacc acaccgtccg tcgctgcgtc ttcgagcgcg
     4381 accgccttct cgctcggcgt gtagctgcgc gcaatccgct tgcgcgagct cttcggaagt
     4441 gccgtctcct cgtttggcgc cgacttggtt accgatggcg tcggccgctt ctgtgttgag
     4501 atcgccaccc tcgcgacctt cgcctccttc agcaccttct tcgcctgcct cttcgccgtc
     4561 gtcaccagcc ttttcgtcgc gaccggtgtt gttgtcgacc ccaccttcct cacagtcgtc
     4621 cttgtagatt cgcgctcggc cacctttttc gctgcaatct tcgtcgcgtc gcgattgagc
     4681 cagttgctca ccgtcgtctg tggaacgccg tgatgccgcg ccgcctcgac ccctcccacc
     4741 tcggccgccg tttcaagaat ctcgtcgcgc tcctctatcg tgtactgccg tcgcttccgc
     4801 cgttgattct cttccttgcc catcttcgtc ctcgttggga cgagcaccca ccctcagttc
     4861 gtccggggtt tcaggcctcg atcaccgtgg aagatgttcg gcaacagcac gtaggatcgc
     4921 tcgtggcgct cagatgctcg tcgcccggtc tatgttgtgc acgagagtgc agctcggagc
     4981 acggtggtgg tgtgaagtgg tggggagcga tcacgcacgt tgtagccgcc gcgtccacgt
     5041 aactcgcgat ggaccgctgt tggtgatcgc gcgtgcaata gatcaggtca cggaatgagc
     5101 ccggcggtcg tcagcttcgc atgaagcgcc tgcggcacgc gcgcctccat ctcgatgtcc
     5161 atgcgatctc ggcgccggcg aactcgccag ttgccgacga gccggaggtc gccgtggaat
     5221 cgctcgcgtt tgcggacgac gaggcgatcg aaccacgcga gaaccgcggc agggatccga
     5281 tcgggtgcga gctcggcgcg cacttcggcg acatcgtcgg ccgagtcgtg aagctcctcg
     5341 gtcacgacca agttccacac gttgatcgcg aatcccagca gggaacgcat ttggtgttcc
     5401 tcgatgggcc ccccgtcgct gtcgatcagc ggccgcgcga actccagcag tgtttcggag
     5461 agctttcgcc cggcgccgag agcgcgatcg aggtcacggt gtgggagcgg ctgcccgagc
     5521 tcgtcgaggc cgagctcgcg gcggagctgg agaggatcca ggtcgtcgac gggcttgcgc
     5581 gcgtggtggt tctccatctc cttcaagagc aggtggacat cacgtcgtcc gtctgcatcc
     5641 cgcacggaat ctcgcggagt gtgccctctg agtttcgcga gaggcatgtc gagccacgcg
     5701 cgaaacgcat cgtgcaagga cggcgagccc gaggtcatgg cctgtccatc aatcgtgacg
     5761 gtctcgccgc cgaacatcgt cggcaacgcc tcgcgcgtgc gcttcttcca tgttgcaaga
     5821 ctgccgagca cctcgcgcac gcgattcacg agcgcttctg cgcgctcgat cgagttcgtc
     5881 ctgacaatca accgagtggg cgtgagccgt accgagccga cgatggtgtt cttccaccaa
     5941 gcgtggatcg tgttg
//
LOCUS       DATLBZ010000385         5595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_455779, whole genome shotgun sequence.
ACCESSION   DATLBZ010000385 DATLBZ010000000
VERSION     DATLBZ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455779"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VMZ53_19645"
     CDS             <1..414
                     /locus_tag="VMZ53_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30738.1"
                     /translation="ARIGTHAAVGTEAKLTFTDEETVRYTGLTGKLYVESVDLTVLAE
                     GQLVHRNVKAANDALNQLVGYVMASKPLPKSLQLDVGVGHFTQDTRVKGLYRDCVDVN
                     LHWYATTHIELLLTTRLEIVDGTGGYALTQLHYRL"
     gene            complement(415..1380)
                     /locus_tag="VMZ53_19650"
     CDS             complement(415..1380)
                     /locus_tag="VMZ53_19650"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS30739.1"
                     /translation="MKWVKRIAMVLGVLLVLAVGFVVVFFYVLHPKSRSAPSMTAPNT
                     PEAIARGEYLAQHVLGCALCHSPIDESKPGDEVLAGQLFAGRVFPVKEMGFPGTLNGP
                     NLTPDKTAGIGAWTDGEVARAIREGVDRNGKTLFPMMPYTRFKRLTDEDTLAVIAYLR
                     SVPPIATKVPRSEINFPVSMFIRLAPAPLAGSPPAWPTDTVERGKILLEVMSCIDCHT
                     PMVKGKLVDGREFSGGNRFKGAFGDVHSSNITPDQATGIGAYSDDDLMRVFKEGKGKD
                     GRTLWVMPWRALQGTKDEDLRAVIAALRTLKPIQNVVPAPNVTAK"
     gene            complement(1383..1721)
                     /locus_tag="VMZ53_19655"
     CDS             complement(1383..1721)
                     /locus_tag="VMZ53_19655"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUS30740.1"
                     /translation="MGKAASLCLVIAFTTGCRSSKASEPMDDGEATFRNICAKCHGQT
                     GSGGLPLTPGGAAPRDLTDPAWHVTRTDAELEQFVREGKAPMPAFKTLLTTEQIRAVV
                     GKVRRLRKDR"
     gene            1874..3847
                     /locus_tag="VMZ53_19660"
     CDS             1874..3847
                     /locus_tag="VMZ53_19660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUS30741.1"
                     /translation="MGTKIETSSETIAGLQGELERRGDQLALANRKGAEASELFRLLV
                     SSVRDYAIFLLDPSGRVATWNAGAERLKGYRAEEIIGLHFSKFYPEVDIRAGKCEMEL
                     EVAAREGRFEDEGWRLKKDGTPFWANVIITALRDSNGMLVGFGKVTRDLTERKHAEEL
                     RLAAEERFRFLVESVKDYAIFILDPRGYVATWNTGAERIKGYRAEEIIGQHFSKFYPP
                     EELYKTEFELQVAEKEGRFEDEGFRLRKDGTKFWANVLISPIRNREGTLIGFSKVTRD
                     LTDRKAREEDRAARLAAEQANRTKDEFLAMLGHELRNPLAPIVTALQLIKLRGDMTAK
                     KEYEVIERQVTHMMHLVDDLLDVSRVTRGKIDIKRVRLDIRAPIAKAIEIASPLMEER
                     RHQFEVNVPPHGILVDGDEARLTQVFANLLTNAAKYTDRGGSVVLTVQQSADEVSVEV
                     RDNGQGIEPELLAKVFEPFVQGYQTPDRAVGGLGLGLALVRSLVGLHEGRVEAHSPGR
                     GKGSRFVVTLPSVGELAPPPPSGEAGAFAATGNRQRILLVDDNADARLLLSEVLTAVG
                     HDVRTAGDGPEALALLETFQPDVAVLDIGLPVMDGYELADQLRAKMAKPPRLIALSGY
                     GQPGDQEKSVAAGFEQHLVKPVDLKNLLASIGR"
     gene            complement(3844..4227)
                     /locus_tag="VMZ53_19665"
     CDS             complement(3844..4227)
                     /locus_tag="VMZ53_19665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS30742.1"
                     /translation="MPTEGVGVRLLIVEDDPDIRESLGELLAAEGFDVSLAENGKIAL
                     DHLAGCDTLPQVILLDLMMPVMSGEQFRLAQLADERYAKIPVVIMSAARELTQTGERL
                     RVAAVLKKPLGIEPLVESLRRAALP"
     gene            4364..4771
                     /locus_tag="VMZ53_19670"
     CDS             4364..4771
                     /locus_tag="VMZ53_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30743.1"
                     /translation="MMTAPAEELVRELAVEILDSFEKLELVNCLVRKGAAMNADELAA
                     ALRIERSNLVEPLASLAREQIISSNAKGYEIVDGGAWTRHVRELAKLYDDDRIAVVTI
                     MSEAALTRLRRQANRAFADAFVIRAKKKGDQDG"
     gene            4764..5024
                     /locus_tag="VMZ53_19675"
     CDS             4764..5024
                     /locus_tag="VMZ53_19675"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HUS30744.1"
                     /translation="MAEVVYALCAFTSLVCAVLLGRAYAGSKQRLLMWSTLCFAGLFI
                     NNVLTFIDLALVPTAMDLHWLRSSVGFVAVALLAVGLIWETK"
     gene            5021..5299
                     /locus_tag="VMZ53_19680"
     CDS             5021..5299
                     /locus_tag="VMZ53_19680"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HUS30745.1"
                     /translation="MSLQALATFLDGGRVVFCLIIALCFWRLGRTTRDRLYHAFAIAF
                     VLMAVSATLVGLRVAIADWSAIIYLPRLLAFLVIIWAIVDKNRRAPKE"
     gene            5323..>5595
                     /locus_tag="VMZ53_19685"
     CDS             5323..>5595
                     /locus_tag="VMZ53_19685"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30746.1"
                     /translation="MEAAPERFGSYVVYEQLGKGGMATVHRAELQQRDGTTKQIALKR
                     LMPTYQKEIVALFLDEARLLRYLHHPNIAETYDSGKVFGTYFIAMEY"
BASE COUNT         1025 a         1795 c         1783 g          992 t
ORIGIN      
        1 gcacgcatcg gcacgcacgc cgccgtcggc accgaagcaa agctcacgtt caccgacgag
       61 gaaaccgtcc gctacacggg gctcaccggc aagctgtatg tcgagagcgt cgatctcacc
      121 gtgctcgccg agggacagct cgtgcatcgc aacgtgaaag cggcgaacga tgcgctgaac
      181 cagctagtcg gctacgtgat ggcgagcaag ccgctgccga agagcctaca gctcgacgtc
      241 ggcgtgggtc acttcacgca ggacacgcgc gtcaaaggtc tctatcgcga ctgcgtcgat
      301 gtgaacctcc actggtacgc gacgacgcat atcgagctgc tcctcacgac acggctcgag
      361 atcgtcgacg gcaccggcgg ctacgcgctc acccagctgc actatcgtct ctagttactt
      421 cgcggtcacg ttcggcgccg ggaccacgtt ctggatcggc ttgagcgtgc gcagtgcggc
      481 gatcacggcg cgcagatctt cgtccttcgt gccttggagc gcgcgccacg gcatgaccca
      541 cagcgtgcgg ccgtctttgc ctttgccttc tttgaacacg cgcatgagat cgtcgtcgct
      601 gtacgcgccg atgcccgtgg cttgatccgg cgtgatgttc gacgagtgaa cgtcgccgaa
      661 tgcgcccttg aacctgttgc caccggagaa ctctctgccg tcgacgagct tgcccttgac
      721 catcggcgtg tgacagtcga tgcagctcat gacctcgagc aagatcttgc cgcgctcgac
      781 ggtgtcggtc ggccatgcgg gcggcgagcc ggcgagcgga gcgggcgcca ggcggatgaa
      841 catcgacacc ggaaagttga tctcgctgcg cggaaccttg gtcgcgatcg gtggcaccga
      901 gcgcaggtag gcgatcacgg cgagcgtgtc ctcgtcggtg agccgcttga accgcgtgta
      961 cggcatcatc gggaagagcg tcttgccgtt gcgatcgacg ccctcgcgaa ttgcccgcgc
     1021 gacttcgcca tccgtccacg cgccgatgcc cgcggtcttg tcgggcgtca agttcgggcc
     1081 gttcagcgtg ccgggaaatc ccatctcctt cacggggaac acgcgccccg cgaacagctg
     1141 accggcgagg acctcgtcgc cgggtttgga ctcgtcgatc ggcgagtggc agagcgcgca
     1201 gccgagcacg tgctgagcga ggtactcgcc acgcgcgatc gcctcgggcg tgttgggcgc
     1261 cgtcatgctc ggggccgagc gcgatttcgg atgcagcacg tagaagaaga caacgacgaa
     1321 gcccaccgcg agcacgagca acacaccgag caccatcgcg atccgtttca cccatttcat
     1381 cgctatcgat cctttcgcaa acgccgcacc ttgccgacca ccgcgcgaat ctgctcggtc
     1441 gtcagcaatg tcttgaacgc cggcatgggg gccttaccct cgcgaacgaa ctgctcgagc
     1501 tccgcgtcgg ttcgcgtgac gtgccacgca ggatccgtca gatcgcgagg agccgcgccg
     1561 cccggcgtca gaggaagccc gccacttccc gtctgaccgt ggcacttcgc gcagatgttg
     1621 cgaaacgtcg cttcgccgtc atccataggc tccgaggctt tgctcgaacg gcaaccggtc
     1681 gtaaacgcga tcacgagaca gagactcgcg gctttcccca aggcgaccca tcgtaccctt
     1741 cgggtgcttc cccaataagg ccggatgcgc ggattcgctc gctgtgcgca tgcgcgcggt
     1801 tgccgtgcgg aattgttaag ttcagttacc ggctggaaaa accggattat gctcggctgc
     1861 ggagcgtcgc tgtttgggta ccaagatcga aacgagcagt gagaccatcg ccggtctcca
     1921 gggcgagctc gagcgtcgag gcgatcagct ggcgctcgcg aaccgcaaag gcgcagaagc
     1981 gtccgagctg tttcgcctgc tcgtctcgag cgttcgcgac tacgcgatct ttcttctcga
     2041 tccgagcggc cgcgtagcga cctggaatgc cggcgccgag cgactgaagg gctatcgcgc
     2101 cgaagagatt atcggcctgc acttctcgaa gttctacccc gaggtcgata tccgcgcggg
     2161 taagtgcgag atggagctcg aagtcgcagc gcgcgaaggc cgcttcgagg acgagggctg
     2221 gcgtctcaag aaagacggca cgccgttttg ggcgaacgtg atcatcacgg cgctccgcga
     2281 ttcgaacggc atgctcgtcg ggttcggcaa ggtcacgcgc gatctgacgg agcgcaagca
     2341 cgccgaggag ctgcggctcg cggccgaaga gcgctttcgc tttctcgtcg agagcgtcaa
     2401 agactacgcg atcttcatcc tcgatccgcg cggctacgtc gcgacgtgga acaccggcgc
     2461 cgagcgcatc aagggctacc gagccgaaga gatcatcggc cagcacttct cgaagttcta
     2521 tcccccggag gagctctaca agacggagtt cgagctccag gtcgccgaga aagagggccg
     2581 cttcgaggac gaaggctttc gcctgcgcaa ggacggcacg aagttctggg cgaacgttct
     2641 catcagcccg attcgcaatc gcgagggcac gctgatcggg ttctcgaagg tcacgcgtga
     2701 tctcacggat cgcaaggcgc gcgaagaaga ccgcgctgca cgtctggccg ccgaacaggc
     2761 gaaccgcacg aaggatgaat tcctcgcgat gctcggtcac gagctgcgca atccgctcgc
     2821 accgatcgtc accgcgttgc agctgatcaa gctgcgcggc gatatgaccg cgaagaagga
     2881 atacgaagtc atcgagcgtc aggtcacgca catgatgcac ctcgtcgacg atcttctcga
     2941 cgtctctcgc gtcacgcgcg gcaagatcga catcaagcgc gtgcgcctcg atattcgcgc
     3001 accgatcgcg aaggccatcg agatcgcgag ccctctcatg gaggagcggc gtcaccagtt
     3061 cgaagtaaac gttccgccgc acgggatcct cgtcgatggc gacgaagcgc gcctgacgca
     3121 ggtctttgcg aacctcctga cgaatgccgc gaagtacacc gaccgcggcg gcagcgtcgt
     3181 gctgacggtg caacagagcg ccgacgaggt cagcgtcgag gtgcgcgaca acggccaagg
     3241 catcgagccc gagctattgg caaaagtctt cgagccgttc gtgcagggct atcaaacgcc
     3301 cgaccgagct gtcggcggtc ttggcctcgg tctcgcgctc gtgaggtctc tcgtgggtct
     3361 gcatgaaggc agggtcgagg cccacagccc gggcagaggc aaaggcagcc ggttcgtcgt
     3421 cacgttgcca tcggtcggcg agctcgctcc gccgccaccg tccggagagg ccggcgcatt
     3481 cgcggcgaca gggaatcgcc agcgcatcct gctcgtcgac gataacgccg atgcacgact
     3541 cctcttgtcg gaggtgctga ccgcggtcgg ccacgatgtt cggaccgccg gcgatggtcc
     3601 ggaagcgctc gctctgctcg agacgtttca acccgacgtc gccgtcctcg acatcggact
     3661 tcctgtaatg gacggctacg agctcgcaga ccagctccgt gcaaagatgg cgaagccgcc
     3721 acgcctcatc gcgctgagcg gctatgggca gcccggcgat caagaaaaga gcgtcgcggc
     3781 cggcttcgag caacatctcg tgaagcccgt ggatctgaag aatctgctcg cgtcgatcgg
     3841 acgctagggc agggctgctc gccgcagcga ctcgaccagc ggctcgatgc cgagcggctt
     3901 cttgagtacc gcggccacac gcaggcgctc acccgtttgg gtaagctcgc gagcagcgct
     3961 catgatcacg accggaatct tggcgtagcg ctcgtctgcg agctgcgcga gccggaactg
     4021 ctcgccactc atcacgggca tcatgaggtc cagcaggatg acctgtggga gcgtgtcgca
     4081 gcctgcaaga tggtcgagcg cgatcttgcc gttctccgcg aggctcacgt cgaaaccctc
     4141 tgcagcgagc agctcgccca gcgactcccg aatatcggga tcgtcctcga caatcaacaa
     4201 tctcacgcct acgccttccg tcggcaatcc tgcgcagact ccacggcact gtcaacaccc
     4261 tgaaacggtg gctcgactgt gcgctagcgc tcacttggtc gatcgcaggg caacctcggg
     4321 aacgctcgct tgcgcacccc agatcgtttg tgctccaacg aatgtgatga cggcgcctgc
     4381 cgaagagctc gttcgcgagc tggcagtcga gatcctcgat tcgttcgaga agctcgagct
     4441 ggtgaactgt ctcgtgcgca agggcgcggc gatgaacgcc gacgagctcg ccgcggcgct
     4501 tcgcatcgag cggagcaatc tcgtcgagcc gctcgcatcg ctcgcacgcg agcagatcat
     4561 ctcttccaat gccaagggct acgagatcgt ggatggcggc gcgtggacgc gacacgtgcg
     4621 cgagctcgcg aagctctacg acgacgatcg catcgcggtg gtcacgatca tgtcggaggc
     4681 ggcgctgacg cggctgcggc ggcaggccaa ccgggcgttc gccgacgcgt tcgtgatccg
     4741 tgcgaaaaag aaaggcgacc aagatggctg aagtggttta cgcgctctgc gcgttcacga
     4801 gcttggtgtg cgccgtatta ctcggccgcg catacgcggg ctcgaagcag cgcctcctca
     4861 tgtggagcac gctgtgcttc gcggggctct tcatcaataa cgtcttgacg ttcatcgatc
     4921 tggccttggt gccgaccgcg atggacctgc attggttgcg cagctcggtc gggttcgtgg
     4981 cggtcgctct gttggcggtc ggcctgatct gggagaccaa atgagcctcc aggcactcgc
     5041 cacgttcctc gatggtgggc gcgtcgtgtt ctgcctgatc atcgcgctgt gcttctggcg
     5101 gctcggccgc acgacgcgcg accggctcta tcacgcgttc gcgatcgcgt tcgtgttgat
     5161 ggccgtgagc gctacgctcg tcgggctgcg cgtcgcgatc gccgattgga gcgccatcat
     5221 ctacttgccg cgcctcctcg cgttcctcgt gatcatctgg gccatcgtcg acaagaatcg
     5281 gcgagccccc aaagagtgac gcgttgacct agaatagggc aggtggaagc cgcgccagaa
     5341 cggttcggct cgtacgtggt ctacgagcag ttgggcaagg gcggcatggc gaccgtgcat
     5401 cgcgccgagc tccagcagcg cgacggcacg acgaagcaga tcgcgctcaa gcggctgatg
     5461 ccgacgtatc aaaaggagat cgtcgcgctg ttcctcgacg aggctcgcct gcttcgctac
     5521 ctccaccacc cgaacatcgc ggagacgtac gactccggga aagtgttcgg cacgtacttc
     5581 atcgcgatgg agtac
//
LOCUS       DATLBZ010000386         4947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8424878, whole genome shotgun sequence.
ACCESSION   DATLBZ010000386 DATLBZ010000000
VERSION     DATLBZ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8424878"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VMZ53_19690"
     CDS             <1..1259
                     /locus_tag="VMZ53_19690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HUS30747.1"
                     /translation="LPVARFLGALARAQGAGLSWSWGPADDAPFTPRVLAGRCVLCRA
                     RWRLSAQDVAALRAGDSGVRERWQLPRWIVVRDGDRELPIDLDNAYLREALLGEAATV
                     VREMFPAADELGLDGDDGRFVHELQATYVRRDPVRAARFPTATAAQRSFPPGSEWLYV
                     KWYAGARGVDEILAAIAPVVRELTAAGVSKRWFFVRFADPDWHLRVRLHGEPATLLAT
                     VLPALSRAVEPLREAGVIWKTQLDTYERELERYPAIEAAEHVFAADSDAVLAILEAYD
                     DPFDLRRLAMCGVDRLLGDLGLDEAARLALVTRMRDHLGAEQHLGTDGKRRFGELFRE
                     REAALVAALTGEDEELALGRSALDGRSAAIAPVIDHLRGPEVAASLAHLHVNRMFAVA
                     PRQQELVVYELMRRTYERLRYTRRSG"
     gene            1252..2994
                     /locus_tag="VMZ53_19695"
     CDS             1252..2994
                     /locus_tag="VMZ53_19695"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS30748.1"
                     /translation="MGESRSPDATATVTPESPETMESGGPGTLRLVIAGDGVYSTESL
                     PATGKITVGRADDNDITVDDGSISRHHAQIFIERPLRVEDLGSANGTWVREVRLAPNS
                     PQVISVNEAIRVGTVTLIVQGRPAKIRTRRLRDHGYFEDRVEDECARATRAGGNFVIV
                     HIVLHRTDLDLQPTFAAALRESDVVATYSPGELEILLVDSTPDQAANAVSRLEQELST
                     HSVPARIGTAWFPRDGRDASTLMSRARERASGTASRIEANEIVVTDEQMTALYQLIER
                     IAATDISVLLLGETGVGKEVVAETLHRRSARATKPFLRLNCGALTETLLESELFGHER
                     GSFTGATQTKPGLFEVAEGGVLFLDEVGELLPSTQVKLLRVLDERKVMRVGGIKPRDI
                     DVRVIAATNRDLDAEVDRGTFRVDLLYRLNAMSIVIPPLRERVAEIVPLARTFLRTFA
                     AKLGRATPALSTDATALLMSYSWPGNIRELRNVIERAVVLCHNDVVEAHDLPEDKMRT
                     TFTTDIRTAPIPVVRVDEPASDGERDERQRILMALEQCAGNQTTAAKLLGISRRTLIN
                     KMEKFAVPRPRKRT"
     gene            complement(2996..4015)
                     /locus_tag="VMZ53_19700"
     CDS             complement(2996..4015)
                     /locus_tag="VMZ53_19700"
                     /inference="COORDINATES: protein motif:HMM:NF019840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30749.1"
                     /translation="MRFLGLFLVSALATSILACAVDPVGSADADDDDDDVTVVPPADQ
                     PASPSVTRCLDVPDAGPTEDWRHLSSNLIAELGDAHHRGNDLIATTDDALQTLAGRLT
                     YGKTDKDLEDEDADVFACIAGSWKKLARVTTDGEGRFSLSLSGAQRLPEGMRDLYLSV
                     VGDRTGVPFVAFVAKPGAAVVVSDVDGTLTSSENAYPTSLVLGGDVAAQPDAAARLRL
                     AAEHGATIVYLTTRGDRFTQDTRSWLAAKGFPYGALRMPTSIITVPGEDTIEAKTSML
                     GDLAGFTLLAGVGNRHTDVEAYTNAGLDPQHIFIKLPEFESELTDDLATGRATRIDQY
                     LAMPM"
     gene            complement(4103..4585)
                     /locus_tag="VMZ53_19705"
     CDS             complement(4103..4585)
                     /locus_tag="VMZ53_19705"
                     /inference="COORDINATES: protein motif:HMM:NF019057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HUS30750.1"
                     /translation="MRSLNNIVTVLVLSFASISAVGCAAGGDELSDVDNTESSATAPG
                     KVDLYQSSDAQWRFRVVAGNGRVLLSSEAYVSKQGAENGLASVLENGVDPAQYQLNLA
                     ANGKYNLRLRAQNFEVIAFTQTYATKSSATRAIGSCVRAIETYLDATVGSSASQQSAE
                     "
     gene            complement(4718..>4947)
                     /locus_tag="VMZ53_19710"
     CDS             complement(4718..>4947)
                     /locus_tag="VMZ53_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HUS30751.1"
                     /translation="RAHVSRFDADGAVMFFTFEKDGGPADDTTCAALEKSAEQAGAWL
                     LGARATKLDSYLRALRDTLDPQGIMNPGTLS"
BASE COUNT          808 a         1598 c         1720 g          821 t
ORIGIN      
        1 tgctgcccgt cgcgcggttc ttgggcgcgc tcgcacgagc acaaggggcg ggactttcgt
       61 ggagctgggg ccctgccgat gatgcgccgt tcacgccgcg cgtgctcgca ggccgttgcg
      121 tgttgtgtcg cgcgcgttgg cggctgagcg cccaggacgt tgccgcgttg cgagcggggg
      181 acagcggcgt gcgcgagcga tggcagctgc cgcgctggat cgtcgtgcgt gacggcgacc
      241 gcgagctacc gatcgatctc gacaacgcgt acctgcgcga ggcgcttctc ggcgaggccg
      301 cgacggtcgt gcgcgagatg ttcccggccg ccgatgagct cggcctcgac ggcgacgacg
      361 gccgcttcgt ccacgagctg caagcgacgt acgtgcgtcg tgatccggtg cgtgcggcgc
      421 ggttcccgac ggcgaccgcc gcgcagcgca gctttccgcc cggcagcgag tggctctacg
      481 tgaagtggta cgccggtgcg cgtggggtcg acgagattct cgctgccatc gcgccggtcg
      541 ttcgcgagct gacggccgct ggcgtttcga agcggtggtt cttcgtccgg ttcgcggacc
      601 ccgactggca cctccgcgtg cgcctgcatg gcgagccggc aacgttgctg gccacggtgc
      661 ttcctgcgtt gtcgcgagcc gtcgagccgt tgcgcgaggc cggtgtcatc tggaagacgc
      721 agctcgacac ctatgagcgc gagctcgagc gctatcccgc gatcgaagcg gccgagcatg
      781 tgtttgccgc ggatagcgat gcggtgctcg cgatcctcga ggcgtatgac gatccattcg
      841 accttcggcg cctggcgatg tgcggcgtcg atcggctgct cggcgatctg gggctcgacg
      901 aagcggcgag gctcgcgctc gtcacgcgca tgcgcgatca cctcggtgcg gaacagcacc
      961 tcggtaccga cggcaagcgc cggtttggcg agctgttccg cgagcgcgag gcggcgctgg
     1021 tcgcggccct caccggcgag gacgaagagc tcgctctcgg gcggtccgcg ctggatggcc
     1081 ggagcgccgc tatcgcgcct gtcatcgatc acctgcgtgg gcccgaggtg gcggcgtcgc
     1141 tggcgcacct gcatgtcaat cgcatgtttg cggtggcgcc tcgccagcag gagctcgtcg
     1201 tgtacgaact gatgaggcga acctacgaac gcctacggta tactcggcga agtgggtgaa
     1261 tcgcggtctc ctgacgccac cgcgacggtg acgcctgaat cgcccgagac gatggagagt
     1321 ggcggtccgg gaacgctgcg gctcgtgatc gccggcgatg gcgtctattc gaccgagagc
     1381 ttgccggcga ccggaaagat cacggttggc cgcgcggacg acaatgacat caccgtcgac
     1441 gacggctcga tctcgcgcca tcacgcgcag atcttcatcg agcggccgct gcgcgtcgaa
     1501 gatctcggca gcgcgaatgg cacgtgggta cgcgaggtga ggctcgcgcc gaattcgccg
     1561 caggtgatca gcgtcaacga agcaattcgc gtcggcaccg tgaccctcat cgtgcaaggc
     1621 aggcccgcga agatccgcac gcgaaggctt cgcgaccatg gctacttcga ggatcgcgtc
     1681 gaggacgaat gcgcacgtgc gacgcgcgcg ggcggcaact tcgtgatcgt ccacatcgtg
     1741 ctgcatcgaa ccgatctgga cctgcagcca acgtttgcgg cggcgctgcg cgaatccgat
     1801 gtggtcgcga cgtactcgcc cggtgagctg gagatcctgc tcgtcgactc gacgcccgac
     1861 caggcggcga acgcggtgag caggctcgag caggagctgt cgacgcacag cgtccctgcg
     1921 cggatcggaa cggcatggtt cccgcgcgat ggccgtgatg cgagcacgct catgtctcgc
     1981 gctcgcgagc gcgcatcggg gacagcgtct cgcatcgagg ccaacgagat cgtcgtcacc
     2041 gacgagcaga tgacggcgct ctaccagctg atcgagcgca tcgctgccac cgacatcagt
     2101 gtgctgctcc tcggcgagac cggcgtcggc aaagaggtcg tggccgagac gctgcatcgg
     2161 cgatctgcgc gcgcgaccaa gccgttcttg cggctgaact gcggcgctct caccgagacg
     2221 ctcctcgaga gcgagctgtt tggccacgag cgcggctcat tcaccggggc gacgcagacg
     2281 aagccgggcc tgttcgaagt cgcggaaggc ggcgtgctgt tcctcgacga ggtcggcgag
     2341 ctgttgccgt cgacgcaggt gaagctcttg cgcgtgctcg acgagcgaaa agtgatgcgc
     2401 gtcggtggca tcaagccgcg cgatatcgac gtgcgcgtga tcgcagccac gaaccgcgat
     2461 ctcgatgcag aggtcgaccg cggcacgttc cgcgtcgatc tgctctatcg cttgaatgcg
     2521 atgtcgatcg tgattccgcc gctgcgcgag cgtgtcgccg agatcgtgcc gctcgcacgg
     2581 accttcttgc gcacgttcgc cgcgaagctc ggacgcgcaa cgccggctct ctcgacggat
     2641 gccaccgcgc tgctcatgtc gtacagctgg cccggcaaca tccgcgagct gcgcaacgtg
     2701 atcgagcgcg cagtcgtgct ctgccacaac gacgtcgtcg aggcacacga cctccccgag
     2761 gacaagatgc ggacgacgtt caccacggac atccgcaccg cgccgattcc ggtggtgcgc
     2821 gtcgacgagc ctgccagcga tggcgagcgc gacgaacgcc agcggatctt gatggcgctc
     2881 gagcagtgtg ccggcaatca gacgaccgcg gccaagctcc tcggcatctc gcgccggacg
     2941 ctgatcaaca agatggagaa gttcgccgtg cctcgcccgc gcaagcggac gtgagctaca
     3001 tcggcatcgc gaggtactgg tcgatgcgcg tcgcgcgacc cgtcgcgagg tcgtccgtca
     3061 gctcgctctc gaactccggc agcttgatga agatgtgctg cggatcgagg cccgcgttcg
     3121 tgtacgcctc tacgtcggtg tgacggttgc cgacgcccgc gagcagcgtg aagcccgcga
     3181 ggtcgccgag catcgaggtc tttgcctcga tcgtgtcttc gcccgggacc gtgatgatcg
     3241 aggtcggcat ccgcagcgcg ccgtacggga agcccttcgc ggcgagccag ctgcgcgtgt
     3301 cctgcgtgaa gcgatcgccg cgggtcgtga ggtacacgat cgtcgcgccg tgctcggccg
     3361 cgagcctgag ccgtgctgct gcatccggct gcgccgcgac atcgccaccg agtacgagcg
     3421 acgtcggata cgcgttctcc gatgaggtca gcgtgccatc gacatcggac acgacgaccg
     3481 ccgcgcccgg cttcgccacg aacgcaacga acggcacgcc cgtgcggtcg cccacgaccg
     3541 agagatagag gtctcgcatg ccttcgggca ggcgctgcgc acccgagagc gagagcgaga
     3601 agcggccttc gccgtcggtc gtgacgcgcg cgagcttctt ccacgatccc gcgatgcacg
     3661 cgaacacgtc cgcgtcttcg tcttcgagat ccttgtccgt cttgccgtac gtcaaacggc
     3721 cggcgagcgt ttgcagcgcg tcgtcggtcg ttgcgatcag gtcattgccg cggtgatgcg
     3781 cgtcaccgag ctcggcaatc aggttgctcg acaggtggcg ccaatcctcg gtcggacccg
     3841 cgtcgggaac gtcgagacaa cgcgtgacgc tcggcgacgc cggctggtcg gcaggcggga
     3901 caacggtgac gtcgtcgtcg tcgtcgtctg cgtccgccga gccgacggga tcgaccgcac
     3961 acgcaagaat cgaggtcgcg agagccgata cgaggaagag acccaggaag cgcatcggtg
     4021 gcaaatatct gcagccgccg gaccacccgt aactacgcga aataccccat cgaaactgtc
     4081 aggtcttcga cgggagtggc gactactcag cgctctgctg gctcgccgac gaacccaccg
     4141 tggcgtcgag gtaggtctcg atcgcgcgga cgcacgagcc aatcgcgcgg gtcgcgctcg
     4201 acttggtcgc gtacgtttgc gtgaacgcga tcacttcgaa gttctgcgcg cggaggcgga
     4261 ggttgtactt gccgttcgcg gcgaggttga gctggtactg agcggggtca acgccgttct
     4321 cgaggacgga cgcgaggccg ttctcggcgc cctgcttcga cacgtacgct tccgacgaga
     4381 gaagcacgcg gccgttgccg gcgacgacgc ggaagcgcca ctgcgcgtcc gacgactggt
     4441 agaggtcgac cttgccgggc gccgtggcgc tcgactcggt gttgtcgaca tcggagagct
     4501 cgtcgccacc agcggcgcag ccgacggcag agatcgaagc gaaggagaga acgaggacgg
     4561 tgacgatgtt gttgaggcta cgcatgatgg acgctgcgta ttacaagggg tgggccaaca
     4621 cctacatgcg gtaaccatct ggaaacgcac gggtgcagat ctcggcggag ttgcacagat
     4681 gcaacttaaa ttgcatccaa gacgcgtctg tgtcgcgcta gctgagagtg cccggattca
     4741 tgatgccctg cggatcgagc gtgtcgcgca gcgcgcgcag gtacgagtcg agcttcgtcg
     4801 cgcgtgcgcc gagcagccac gcacccgctt gctcggccga cttctcgagg gcggcgcatg
     4861 tcgtgtcatc cgcaggcccg ccgtccttct cgaacgtgaa gaacatgacg gcaccatcgg
     4921 cgtcgaagcg cgagacgtgc gcgcggg
//
LOCUS       DATLBZ010000387         5776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4925101, whole genome shotgun sequence.
ACCESSION   DATLBZ010000387 DATLBZ010000000
VERSION     DATLBZ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4925101"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1033)
                     /locus_tag="VMZ53_19715"
     CDS             complement(197..1033)
                     /locus_tag="VMZ53_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30752.1"
                     /translation="MLLCGCDGVFGLTHIHADGDDQVIVAMDAPAADVTISDALTCWL
                     PGLVQYDSDTDGFPDGCDNCPGEANPPQNDEDHDGVGDECDPHLGDAHDHLAFFDGFQ
                     TTDPLWVARGTDGDWKHNVGEWIQTRGISTTNTQLVYGKSFRSPSVAVTFFGQLTPPH
                     ASTVGVFALMDPSSTSNPPDGVLCGLSVTDKTRVHIIELVNGDVANGNEQVGASTLPT
                     SGIAHLLATSRDCTARKAGTVAPTMRVALPTPVAQAMSPIALSAYQSSVRFTSVTVIE
                     TY"
     gene            complement(1113..2564)
                     /locus_tag="VMZ53_19720"
     CDS             complement(1113..2564)
                     /locus_tag="VMZ53_19720"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS30753.1"
                     /translation="MGHVAMTDAELVEASRRGERDAFGQLVSRYQNVVCAVSYSSTGN
                     WALSEDVAQDTFIAAWRQLGQLREIGRLRAWLCQIARNLARKARARDDREAREDAIEV
                     DQDLVARDVDPFSATAQAEVDRVVREALARIPDRYRETLVLYYCENQSVREVATTLGI
                     AEDAAMQRLSRGRRYLADGVTTLVERSLREPPRGRKDLRMAVLAALPHVDPPTATGST
                     SMIKLVIAAAVATAVVGTTAVVAVTHKSDSKPAASLSPSTTPVAQANAPGPLHAIAPP
                     TIPPTAQDGEDQLPPGAIRVEDNPDVISKATRDRLGLERGPSRGPENAPVTIVVFQDL
                     MCPYCGKVLGTIDQLMEEYPGKIRVVLKQFPVHKEAVLAAEASLAAEAQGKFWELHDL
                     MIANQDDLSRDALVAYGKQAGLDEKKLAAALDNHTYKPDLKKEVDAAVEVGVKATPEF
                     LINGKDFTGARPIEDFRANIDAALSEVAAQPQP"
     gene            2694..4643
                     /locus_tag="VMZ53_19725"
     CDS             2694..4643
                     /locus_tag="VMZ53_19725"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30754.1"
                     /translation="METDRTQPGMAGPHPTIAQLRSQSQDKHIHEHVAQCSMCQSVVA
                     ADGDDAPIPLDLPKGLVSESAFKWPADPIARGGMAQIFAGEDRRLGRQVIIKTPRDDD
                     NLPAGMAEMFQHRVSAEARVLAKLQHPSIVTLYELGRATVGWPFLVEERVDGTSLRDR
                     LDELQEQEDADKKPRTRERVELLSNLVAIAEALAYAHERRVVHRDVTPNNIILGKRGE
                     ATLIDWGIARDLDAPASKEPSIHDQSPSATGRMVTISAGTPPYIPLEQSQGRKADPSF
                     DVYSFGVTLYEVASGKTPFVWRYATSPGGRTAQLDAFMAWLQSDDEIPPAMPKDKELS
                     GIIARAMARDPKDRFTAEELVRALKQYLTGDLVFSHRYSLTGRLARWARTHKGATVAL
                     LSLVAFAIGGALVWAQLQRQAKEEAELRAVAASAQVDASEKARLADEATREADAAKAR
                     ADEAVRLGKDAQALKAEADRKRRAADESRKSAEAAASQAKGTADEAVKRFQQAMREKD
                     EANAARESANAAREQAERDRESARAAEVAAQRERDSARTAREQADRDRDAARAAQQAA
                     DKERDAARGAREQAERDREAARAAQQAAEKERDAAIASRANVERERDEARRKAAELEQ
                     RLREQQQQPPPPSGPPGPPPPAPGP"
     gene            complement(4772..5332)
                     /locus_tag="VMZ53_19730"
     CDS             complement(4772..5332)
                     /locus_tag="VMZ53_19730"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HUS30755.1"
                     /translation="MDGPHKRNATYADVEALPEGKVGQLIDGDLYVSGRPRVRHSAAI
                     SGAFAEIVPAMRGRGPKGWHILFEVEIWFGKSQKNLLVPDLAGWRRERMLEIPDVQTL
                     EIVPDWVCEGLSPSTMRLDKGRKREIYEKHKVGHVWYADPKNRMLEVLELTRGSYRLV
                     TVAMGGQQAVLPPFEHEIDVGRFWRR"
     gene            complement(5367..>5776)
                     /locus_tag="VMZ53_19735"
     CDS             complement(5367..>5776)
                     /locus_tag="VMZ53_19735"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HUS30756.1"
                     /translation="RALTSSQRRATLADPEDIFRICEPRVAGLAQEVFIVIGMDIRNG
                     LLDVVEVARGTVHGVEVHPREVFRPLIRMAAAGAVVVHNHPSGDPTPSPHDIELTRRL
                     ITAGDLIGIPLIDHVVIGDRTFRSIKQQLGTEL"
BASE COUNT         1073 a         1905 c         1873 g          925 t
ORIGIN      
        1 ccctcggtga acgcagtgac caccgccccg aacgcgaccg cgaggagcac cgcgaccgcg
       61 agatccagca cgttgccctt gaagatgaag tcccgaaatt ccttcagcat gacgcctccg
      121 gagtcccaag cgttcatccg acgtgccacc cagaaatccc gcagcgccac gcgctagaga
      181 cgtgtaatca cggcgatcag taggtctcga tcacggtgac cgacgtgaac cgcacgctgc
      241 tttgatacgc cgagagcgcg atcgggctca tcgcctgcgc gacgggagtc ggcagcgcga
      301 cgcgcatcgt cggagcgacc gtgccggctt tgcgagctgt gcagtcccgc gacgtcgcga
      361 gtagatgcgc gatgcccgac gtcggaagcg tgctcgcccc gacctgctcg ttgccgttgg
      421 cgacgtcgcc gttgacgagc tcgatgatgt gcacgcgcgt cttgtcggtg acgctgaggc
      481 cgcacaggac gccatctgga gggttcgacg tgctcgatgg atccatgagc gcaaatacgc
      541 cgaccgtcga tgcgtgcggc ggcgtcagct gaccgaagaa cgtcacggcg accgagggcg
      601 agcggaatga cttgccgtag acgagctgcg tattcgtcgt cgagatgccg cgcgtctgga
      661 tccattcacc gacgttgtgc ttccagtcgc cgtcggtgcc gcgcgcgacc cacagcggat
      721 cggttgtctg aaagccgtcg aagaatgcga ggtgatcgtg cgcgtcgccg aggtgaggat
      781 cgcattcgtc gccgacgccg tcgtggtcct cgtcattttg cggtgggttc gcctcgccgg
      841 gacagttgtc gcagccgtcg gggaagccgt ccgtgtcgct gtcgtattgg accaaccctg
      901 gaagccagca cgtgagcgcg tcactgatcg tgacatctgc cgccggcgca tccatcgcga
      961 cgatgacttg atcgtctcca tcggcgtgga tgtgcgtcaa gccgaacacg ccatcgcagc
     1021 cgcagagcag cacgatgcaa acggtgaggc actggacgcg cagaccgcca ttatcgccga
     1081 tcgccgggca caggcgaagt ctccgcgcgc gatcagggct gcggctgcgc tgcaacttcg
     1141 gaaagcgccg cgtcgatgtt cgcgcggaag tcctcgatcg gacgcgcacc cgtgaagtcc
     1201 ttgccgttga tcaagaactc gggcgtcgcc ttcacgccga cttcgacggc ggcatcgacc
     1261 tccttcttga ggtcgggctt gtacgtgtga ttgtcgagcg cagctgcgag cttcttttcg
     1321 tcgagacctg cctgcttgcc gtaggcgacg agcgcgtcgc gagacagatc atcttggttc
     1381 gcgatcatca gatcgtgcag ctcccagaac ttgccttgcg cttcggcagc cagagatgcc
     1441 tccgccgcga gcaccgcttc cttgtgcacg gggaactgct tcagcacgac gcggatcttg
     1501 ccggggtact cctccatgag ctgatcgatc gtcccgagca ccttgccgca gtacgggcac
     1561 atgaggtctt ggaagacgac gatcgtcacg ggcgcattct ctgggccgcg cgacggacca
     1621 cgctcgaggc cgaggcgatc gcgggtggcc ttcgagatca cgtctgggtt gtcctcgacg
     1681 cgaatcgcgc cgggcggcag ctggtcttcg ccgtcttgcg cagtgggcgg aatcgtcggc
     1741 ggtgcgatcg cgtgcagcgg accgggcgcg ttcgcctgag cgacgggcgt tgtcgagggt
     1801 gatagagatg cggcgggctt cgagtcgctc ttgtgcgtga cagcaacaac agcggtggtg
     1861 ccgacgacag cagtcgcgac ggcagcggca attacgagct tgatcataga agtcgatcct
     1921 gtggcagtgg gtggatcgac atggggcagc gcggccaaca cggccatgcg cagatctttt
     1981 cgtccgcggg gtggttcgcg aagcgagcgc tcgaccagcg tggtcacacc atcggcgaga
     2041 tagcgacgtc ctcgactgag gcgctgcatc gcggcgtcct ctgcgatacc cagcgtcgtc
     2101 gcgacttcgc gcaccgactg gttctcgcaa tagtagagga cgagcgtctc gcgatagcga
     2161 tcgggaatgc gtgcgagcgc ttcgcgaacg acgcgatcga cctcggcctg cgcagtggcc
     2221 gagaacggat cgacatcacg tgcgacgaga tcttggtcga cctcgatcgc atcttcgcga
     2281 gcttcgcggt catcgcgcgc tctcgctttg cgggcgaggt tgcgagcgat ctggcaaagc
     2341 cacgcacgca gtctgccgat ctcgcggagc tgaccaagct ggcgccacgc agcgatgaac
     2401 gtgtcttgcg cgacgtcctc gctgagtgcc cagttcccag tcgagctata cgagaccgcg
     2461 cacaccacat tctggtaacg gctgacgagc tgaccgaacg catcgcgttc accgcggcga
     2521 ctcgcttcaa cgagctccgc atctgtcatt gcgacgtgtc ccatgtcctg cactggtgtt
     2581 cccggcggcc cgtcagacct gacaaagaaa atgcattcgt atcgcaaacc accgtgatgt
     2641 gaagaagaat tccgtttcgt gaactgaacg tcgcacgcgg ctatgctcgc gaaatggaga
     2701 cggatcgcac gcagccgggc atggcagggc cccacccgac catcgcgcag ctgcgaagcc
     2761 agagccagga caagcacatc cacgagcacg tagcccagtg ctcgatgtgt cagtccgtgg
     2821 tggccgccga tggcgatgat gcgccgattc cgctcgacct cccgaagggc ctcgtgagcg
     2881 agagcgcctt caagtggccc gcggatccga tcgcacgcgg cggcatggcg cagatcttcg
     2941 ccggtgaaga tcgccggctc ggtcgccaag tgatcatcaa gacgccgcgc gacgacgaca
     3001 atctgccggc cggcatggcc gagatgttcc agcaccgcgt gtccgccgaa gcgcgcgtgc
     3061 tcgccaagct ccagcacccg tcgatcgtca cgctctacga gctaggcagg gcgacggtcg
     3121 gctggccatt cctcgtcgag gagcgcgtcg acggcacgtc actgcgcgat cgtctcgacg
     3181 agctgcaaga gcaggaagac gccgacaaga agccacgcac gcgcgagcgt gtcgagctgc
     3241 tgtcgaacct cgtcgcgatc gccgaggccc tcgcgtacgc gcacgagcgg cgcgtcgtgc
     3301 atcgcgatgt cacgccgaac aacatcatcc ttggtaaacg cggcgaagcg acgctgatcg
     3361 attggggcat cgctcgcgat ctcgacgcac ctgcgagcaa ggaaccgtcg attcacgacc
     3421 agagcccgtc ggcgacgggg cgcatggtga cgatcagcgc gggcacgccg ccgtacatcc
     3481 cactcgagca atcgcaaggc cgcaaggccg atccatcgtt cgacgtctac agcttcggcg
     3541 tcacgctcta cgaggttgca tcgggcaaga cgccattcgt ctggcgctac gcgacgtcgc
     3601 cgggcggccg caccgcgcag ctcgacgcat tcatggcatg gctgcagtcc gacgacgaaa
     3661 tcccgccggc gatgccgaag gacaaggagc tgtccgggat catcgcgcgc gcgatggctc
     3721 gcgatcccaa ggatcgcttc acggcagaag agctcgtccg cgcgctgaag caatacctga
     3781 ccggcgacct cgtcttctcg catcgctact cgctcacggg tcggctcgcg cgctgggcac
     3841 gcacgcacaa aggcgcgacg gtcgcgctcc tgtcgttggt cgccttcgcg atcggcggcg
     3901 cgctcgtgtg ggcgcagctg caacgccaag ccaaagagga ggccgagctg cgcgccgtcg
     3961 ccgcgtccgc gcaggtcgac gcatcagaga aggcgcgcct cgccgacgaa gccacgcgcg
     4021 aagccgacgc ggcaaaagca cgcgccgatg aagccgttcg cctgggcaaa gacgcccaag
     4081 cgctcaaagc cgaagccgat cgcaagcgcc gcgcggccga cgaatcacgc aagtccgccg
     4141 aagccgcagc gtcgcaagcg aaaggcacgg ccgacgaagc cgtcaaacgc ttccagcaag
     4201 cgatgagaga gaaggacgag gccaacgcgg cgcgcgagtc tgccaacgcc gcgcgcgagc
     4261 aagccgaacg cgatcgcgaa tccgcacgcg ccgccgaggt cgccgcgcaa agggagcgcg
     4321 actccgcgcg gacagcccgc gagcaagccg accgcgatcg cgacgctgcg cgcgccgcgc
     4381 aacaagccgc agacaaagag cgcgacgccg cgcgcggtgc gcgtgaacaa gccgagcgcg
     4441 atcgcgaagc cgcacgcgca gcacaacaag ccgccgagaa agagcgcgac gccgcgatcg
     4501 cctcacgcgc caacgtcgag cgcgaacgtg acgaagcgcg ccgcaaagct gccgagctcg
     4561 agcaacgcct acgcgagcaa cagcagcaac cgccgccgcc gtcgggcccg ccagggccac
     4621 cgcctcccgc cccgggtccg taggaccgtc ctcgagtgcg gccggcgccg ccacccgcac
     4681 cgggtccgta ggagcgtcct cgagtgctca agggccaccg cctccagccc cgggcgccct
     4741 aagtcgaagt tgagcttgac aggcgtcccc gctatcgtcg ccagaagcgg ccgacatcga
     4801 tctcatgctc gaacggcggc aacacggctt gttgaccgcc catcgcaact gtcaccaacc
     4861 gataggaacc gcgcgtcagc tcgaggacct cgagcatgcg gttcttcggg tcggcgtacc
     4921 agacgtgccc gactttgtgc ttctcgtaga tctctcgctt acggcccttg tcgagtcgca
     4981 tcgtcgacgg cgataggccc tcgcacaccc agtcaggaac gatctccagc gtctggacgt
     5041 cagggatctc gagcattcgc tcgcgacgcc atccagcaag gtcaggcacg agaagattct
     5101 tttgactctt tccgaaccag atctcgacct cgaagagaat gtgccagccc ttgggccccc
     5161 gaccgcgcat ggccggaacg atttcggcga acgctccgga aattgccgct gagtggcgca
     5221 cgcgtggacg accactgacg tacaggtcgc catcgatgag ctgaccgact ttgccttcag
     5281 gcagcgcttc aacgtcggcg tacgtcgcgt tgcgcttgtg tgggccatcc atacgcttct
     5341 tcgagcgtaa cacttgtatc tgcatttcaa agctccgttc cgagctgctg cttgatagag
     5401 cggaacgtcc gatcaccgat gacgacgtga tcgatgagcg ggatgccgat gagatcgccc
     5461 gccgtgatca gacgacgcgt cagctcgatg tcgtgcggtg acggcgtggg atcgcccgac
     5521 ggatgattgt ggacaacgac cgcgcccgcg gcggccatgc ggatgagagg ccggaacact
     5581 tcgcgcgggt ggacttcgac gccgtgcacc gtgccgcgcg cgacttcgac gacatcgagc
     5641 aggccattgc gaatgtccat cccgatcacg atgaagacct cttgtgcgag gccggcaacg
     5701 cgtggctcgc agatgcggaa gatgtcttcg gggtcggcga gcgtcgcgcg gcgttgcgag
     5761 gatgtgagcg cgcggc
//
LOCUS       DATLBZ010000388         8746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5673805, whole genome shotgun sequence.
ACCESSION   DATLBZ010000388 DATLBZ010000000
VERSION     DATLBZ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5673805"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VMZ53_19740"
     CDS             complement(<1..1097)
                     /locus_tag="VMZ53_19740"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS30757.1"
                     /translation="MRRAALLFLVAATTIAHAQNADLTKEFQAGVDAYRLGKFDEART
                     HLEKARDLDPKLPGPYRFLSAVAQAQGKWDDCIGNARKALELNPLSSESADTRKVHDE
                     CRVSAGRPPFRGDLTDSAAVAVTTNVVGATVKINGLTYGATPLAPRPITPGAIEVEIE
                     KQGWKPAKLTPNALPGIVTDVIVDLEPDPNATANELQSQNPGKVDKGWLVLKAPAPDT
                     SLRIDDTQPKSEPDPKEAGAVRIELSPGTHIVEVKSPQTDSWRRRVRISAGQKTLIQP
                     ILVDHDQRAAVEKRGLYILGAGGALLVGGFATAMISRSAAHEARDIQRIETARDPSQP
                     LSVSGMVAPVRTRADFDAATDKAKTFAIISDV"
     gene            complement(1115..3337)
                     /locus_tag="VMZ53_19745"
     CDS             complement(1115..3337)
                     /locus_tag="VMZ53_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30758.1"
                     /translation="MIALEKPPGADAPAASKLQIEKFADGGIACLKFNGTIDESFEGK
                     KIARSIQAETLVLDLGGVKKISSFGIREWVDFASTAVKQVKQMVLIECAPKVVDQLNM
                     VANFHGGGRVFSFYAPFRCDYCDSEQRVLLEVSKDWEAIKSMKLADRPCPSCKDSMYF
                     DEDGSTYFSFILGQGQFELDHDLVAFLAAKLDYRIGTIEQKVRVDKVIEGRLTYLRLG
                     GDLNNSFPRDKLAEGIEGQVILDVAAIPRVEPAGAAEWRGFIQQVTPLVEQTYLTGVQ
                     PAFLEKLCGVEDLGPKGLVLDFTLPYACKSCGTTSQQSIDVAAHYDVLKFATAPELRC
                     PSCKAAMQCMATEAVMTVLPGLPKPAVTKDLERQIGMLKARKIEHKKLSTGLHAVARP
                     GTRSALVPILLVLLLVVGGAAGVLFYMKSKQHEDPGPYGAGVLVEASAKTRPDWITAQ
                     ALPGQAYCKEDGGGLACVGVSMLSPSQEDAEDEASDAAVEAIAFELGKRITDKAWLAT
                     IPAIYQPARDAKLAAMNRDAQSSQARRDVRDGRRAVAHALRGVNPTAAGRYWEAFGGR
                     DGRRMIAFVQVKLSPQDAQQLITTYTTPATALGATAVPFFPELAWKYGKLAKGAVILE
                     LQPGVVQELGLAEHTVVLAVDGRETADAASFAKIVTEEHGLLADRGGSFRLLVQADQG
                     DPREFSTQLAGKQIETTPDKGDGKKHNGGKQNGTGTGGVNVWDRFGGNRGSGRDDPTQ
                     "
     gene            complement(3364..4287)
                     /locus_tag="VMZ53_19750"
     CDS             complement(3364..4287)
                     /locus_tag="VMZ53_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS30759.1"
                     /translation="MHVVILERNKLVGRKVARLFLSIGASAVTVEEPSQASALIESAD
                     VLCADTFDGDFVAEQVRGKPSMRGVLWTAEPLRRSLKYLVETNTINNVLGRKDFESAP
                     RAWEVLMVARRLGSTTPAPLSAYLDWGFSSVDIEVRTTTDRDSAVAQIQMFVGQLNLP
                     KRVAEMFGELGHELLMNAMYDAPVDADGKPKYAADRKADIKLAETERPTVRLATDGSK
                     LVLQVRDPFGRLERRHVFDGLARGLAGGEMDQSHGGAGLGMMVCHNSSSAMFFDVAKG
                     RHTEVTAIFELDMNLRELRTQAKSLHFWSAV"
     gene            4438..5565
                     /locus_tag="VMZ53_19755"
     CDS             4438..5565
                     /locus_tag="VMZ53_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS30760.1"
                     /translation="MRALGAAIALMLISLPAGAEEAKRETTGDKPGELYQTVATKKSR
                     ADLSQFLVKSAEKAAKARDWAKAIPLYQALAVARGPGSPEQKQLATLWNLAGQNEEAA
                     EAWATYANAATDSKEKEQALTEATRLSSTPDPFAEKLSVAALEKDAKQAFNLGRAAFK
                     AKKYGDALVYFHMGYALAPDLPGFLRELGSTYDKLGATDRKREFYRRYLVQRPFGANA
                     DVVRSELARDKDVLGTLLVSSSLPCAELWVNRQKISGKIPDKGLLVAPGNYKGLCFNS
                     KYEMALFEYADVEPGKQAAMSFRWAIIVNKLERPYGRIALENPKSPGTMIDLGITSPE
                     IGVAVPPDGRKLKMVLKDDSGVRTEERTVTIEPGQKLIVQW"
     gene            5578..6792
                     /locus_tag="VMZ53_19760"
     CDS             5578..6792
                     /locus_tag="VMZ53_19760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30761.1"
                     /translation="MVSERPSQEGKLSPGAPNDPVPFGKWYLLGLIARGGMAEVYRAR
                     PQDGAKNLFAVKVMRPQLAREARFIDMFNREGKLAMMLKNRCIVETVEIGQDDGRHYI
                     AMEYVAGRDLTQVLRRCQETQQRIPVPHAVYIAARIAEGLHFAHTLVGPDGRTLNIVN
                     RDVSPSNVRLSYDGDVKLLDFGIAQALMKFTSEIGILKGKFSYMSPEQIRGMPLDART
                     DVFSAGIILHEMLTTEKLFRGDTEFALMEKVRKAEVPPPSNFNRRVTPELDAISLKAL
                     ARDVADRYQSAAQLAADLDALIAGYRFDPKELRQFMRQLFRKEYARELEETETALEAE
                     PGASFGIPNYAQRGPTTGSMQSQETPTSEMQPIPRPGPQAQMAEDPPSVEIQQHEEKP
                     KGFWGSLFKRKK"
     gene            6828..7250
                     /locus_tag="VMZ53_19765"
     CDS             6828..7250
                     /locus_tag="VMZ53_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30762.1"
                     /translation="MAAVPPAPQRPANAEARIAGHAIGVLAGVASLIGGVQALTHHMP
                     PVLGVTMLLMSALLLALTALSFQHYSRIAWSFLSAILVVMALVTLFGSPKVRNLVHIP
                     LGAAMVIPLVLIVGVIALYLSADDYRGTVSQKSQNAAR"
     gene            complement(7205..>8746)
                     /locus_tag="VMZ53_19770"
     CDS             complement(7205..>8746)
                     /locus_tag="VMZ53_19770"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HUS30763.1"
                     /translation="VGGAAACGDELGPNDVEVETGVVRGSDDGAGVKSWLGIPYAAPP
                     VGELRWKPPKRAEAWDGARDGTKVGQKCPQNTVITSGGGIEDCLTLNVWAPSAATGKL
                     PVMFWIHGGAFVFGSGGDPFYSGAELARTRGVVVVTINYRLGGLGFLAHPALAAEDPD
                     HTSGNYGLRDQLAALEWVQRNIGEFGGDPGHVTIFGESAGGFSGCVHYTDPKTAGLFE
                     RAIVESGACLDNGLAQTRQQAESDGLAFGAKVGCPGSDAGAIACMRDLIDYAVLDATA
                     LAPIMMQKPGGFFYDSYPPNTLPNVDGVVIPEQIEKRLSAGDYPKRPIILGTNKDEGT
                     LFHSNILSNLVRDETQYREALMRRFNDAAVVDQIVARYPVGDYPSPNEALAAVSGDVF
                     FTCPARRNARALTAAGSPVYRYVFQRDLEQALFPELHAFHSAEIPFVFGIETYPLGKV
                     GSATSLADAMQSAWTSFAKTGEPGGGWPKYEAASDPVQVLDVPVSQGAGYRAAFCDFW
                     DTVPR"
BASE COUNT         1506 a         2883 c         2806 g         1551 t
ORIGIN      
        1 acgtccgaga tgatcgcaaa cgtcttcgcc ttgtccgtcg ccgcgtcgaa gtccgcacgc
       61 gtgcgcaccg gtgcgaccat gccgctcacc gatagcggct gcgatggatc gcgcgcggtc
      121 tcgatgcgct gaatgtcgcg cgcttcgtgc gccgcgcttc gcgagatcat cgcggtcgca
      181 aagccaccga cgagcaatgc gccgcccgcg ccgaggatgt agaggccgcg cttctcgacg
      241 gccgcgcgct gatcgtgatc gacgaggatc ggttggataa gcgtcttttg gccagccgag
      301 atgcggacac ggcgccgcca cgaatcggtc tgcggtgact tcacctcgac gatgtgtgtg
      361 ccgggtgata gctcgatgcg cactgcaccg gcttcttttg gatcgggctc gcttttgggt
      421 tgcgtgtcgt cgatccgaag cgaggtatcg ggcgcgggcg ccttcaacac cagccagcct
      481 ttgtcgacct tgccgggatt ctgcgactgc agctcgttcg cggtcgcatt cgggtcgggc
      541 tcgaggtcga cgatgacgtc cgtgacgatg ccggggagcg cattcggcgt gagcttcgcg
      601 ggcttccatc cctgtttctc gatctcgacc tcgatcgcac ctggcgtgat cgggcgtggg
      661 gcgagcggcg tggcgccgta cgtgaggccg ttgatcttca cggtcgcgcc gacgacgttc
      721 gtggtcacgg cgaccgcagc gctgtcggtc agatcgccgc gaaacggtgg gcgacctgct
      781 gagacgcgac actcgtcgtg gaccttgcgc gtgtcggcgg actcggagga cagcggattg
      841 agctcgagcg ccttgcgcgc gttgccgatg cagtcgtccc acttgccttg cgcttgcgcg
      901 accgccgaga ggaagcggta cggaccgggc agcttcggat ccaggtcgcg cgctttctcg
      961 agatgcgtgc gcgcttcatc gaacttgccg aggcggtacg cgtcgacgcc ggcctggaac
     1021 tctttcgtca ggtcggcgtt ctgggcatgc gcgatcgtgg tcgcagcgac caagaagagg
     1081 agcgcagcac gcctcatcac gaatgacggt aacactactg cgtcgggtcg tcgcgtccgc
     1141 tgccgcggtt gccgccgaag cggtcccaca cgttgacgcc gcccgtgccg gtgccgttct
     1201 gcttgccgcc gttgtgcttc ttgccgtcgc ccttgtcggg cgtcgtttcg atctgcttgc
     1261 cggcgagctg cgtcgagaat tcgcgagggt cgccttggtc cgcttggacg agcaggcgga
     1321 acgagccgcc gcgatcggcg agcaggccgt gctcctcggt cacgatcttc gcgaacgacg
     1381 cggcatcggc ggtctcgcga ccgtcgacgg ccagcacgac ggtgtgctcc gcgaggccga
     1441 gctcttggac gacgcccggc tgcagctcga ggatgacggc gcccttcgcg agcttgccgt
     1501 acttccaggc gagctccggg aagaacggga cggccgttgc accgagcgct gtcgcgggcg
     1561 tcgtgtaggt cgtgatcagc tgctgcgcgt cttgcggcga gagcttcacc tggacgaagg
     1621 cgatcatgcg gcggccatcg cgcccgccga acgcttccca gtagcggcca gcggccgtcg
     1681 ggttgacgcc gcggagcgcg tgcgcgacgg cgcgacggcc atcgcggaca tcgcgacgag
     1741 cttgcgacga ttgcgcgtcg cggttcatcg ccgcgagctt ggcgtcacgt gcgggctggt
     1801 agatcgccgg gatggtcgcg agccatgcct tgtcggtgat gcgcttgccg agctcgaatg
     1861 cgatcgcttc gacggccgcg tcgctcgctt cgtcttcggc atcttcttgc gaaggcgaga
     1921 gcatcgagac tccgacgcac gcgagaccgc cgccgtcttc tttgcagtac gcctgaccgg
     1981 gcagcgcttg cgcggtgatc caatcggggc gcgtcttcgc gctcgcttcg acgaggacgc
     2041 ccgcaccgta cgggcccgga tcttcgtgct gcttgctctt catgtagaag agcacgcccg
     2101 cggcgccgcc gacgacgagc agcaggacga gcaggatcgg cacgagcgcc gaacgagtcc
     2161 ccgggcgcgc gactgcgtgc agccccgtcg acagcttctt gtgctcgatc ttgcgagcct
     2221 tgagcatgcc gatctggcgc tcgagatctt tcgtgacggc cggcttcgga agacccggca
     2281 gcactgtcat gaccgcttcc gtggccatgc actgcatcgc cgctttgcac gatgggcaac
     2341 gcagctcggg cgcggtcgcg aacttgagga cgtcgtagtg cgcggcgacg tcgatcgatt
     2401 gctggctcgt cgtgccgcac gacttgcatg cgtacggcag cgtgaaatcg aggacgaggc
     2461 ccttcggacc gaggtcttcg acgccgcaca gcttctcgag gaacgccggc tgcacgcccg
     2521 tcagatacgt ctgctcgacg agcggcgtga cctgctggat gaacccgcgc cactccgcag
     2581 cgcctgcggg ctcgacgcgc gggatcgcag cgacgtcgag gatgacttgg ccttcaatgc
     2641 cctctgcgag cttgtcacgc gggaacgagt tgttgagatc gccgccgagg cgcaggtacg
     2701 tcagccggcc ttcgatgacc ttgtcgacgc gaaccttctg ctcgatcgtg ccgatgcggt
     2761 agtcgagctt cgccgcgagg aacgcgacga gatcgtggtc gagctcgaac tggccttggc
     2821 cgaggatgaa cgagaagtac gtcgagccat cctcgtcgaa gtacatgctg tccttgcacg
     2881 acgggcatgg ccggtcggca agcttcatcg acttgatcgc ttcccagtct ttgctgacct
     2941 cgaggagcac gcgctgctcc gagtcgcagt agtcgcagcg gaacggcgcg tagaagctga
     3001 acacgcggcc accgccgtgg aagttcgcga ccatgttcag ctggtcgacg actttcggcg
     3061 cgcactcgat caagaccatc tgcttgacct gcttgaccgc cgtcgacgcg aagtcgaccc
     3121 actcgcgaat accgaacgac gagatcttct ttacgccgcc gagatcgagc acgagcgtct
     3181 cggcttggat cgagcgcgcg atcttcttgc cttcgaacga ctcatcgatc gtgccgttga
     3241 acttgaggca cgcgatgccg ccgtcggcaa acttctcgat ctgcagcttc gaggctgctg
     3301 gagcatccgc accaggcggc ttctcgagcg cgatcatcgg ttgggattta cgcggggaaa
     3361 ccatcaaact gcactccaga aatggaggct cttcgcctgc gtcctgagct cgcgcaaatt
     3421 catgtcgagc tcgaagatcg cagtgacctc cgtgtgtcgc cccttcgcca cgtcgaagaa
     3481 catcgccgac gaggagttgt gacagaccat cattcccaac cctgccccac catgtgactg
     3541 atccatttcg ccgcccgcga gaccgcgcgc gagcccgtcg aagacatgcc ggcgctcgag
     3601 gcgaccgaac ggatcgcgaa cttgtagtac caacttgctc ccgtccgtcg cgagcctcac
     3661 cgtcgggcgc tccgtctcgg cgagcttgat gtccgccttg cgatccgcgg cgtacttcgg
     3721 cttgccgtcg gcgtcgacgg gcgcgtcgta cattgcattc atgagcagct cgtgacctag
     3781 ctcgccgaac atctcggcaa cgcgctttgg cagattgagc tggccgacga acatctggat
     3841 ctgcgcaacc gcgctgtcgc gatccgtcgt cgtgcggacc tcgatgtcga ccgacgagaa
     3901 accccaatcg aggtacgcgg acagcggcgc gggcgtcgtc gagccgaggc ggcgcgcgac
     3961 catgagcact tcccacgcgc gcggtgcgga ctcgaaatct ttgcgaccga gcacgttgtt
     4021 gatcgtgttc gtctcgacga gatacttcag cgaccgacgc agcggttctg cggtccacag
     4081 cacaccgcgc atcgacggtt ttccgcgaac ttgctccgcg acgaagtctc catcgaatgt
     4141 atcggcgcac agaacgtctg cactctcgat gagagcgctc gcctggcttg gctcttcgac
     4201 cgtcacggcc gaagcaccaa tcgacaagaa caaccgtgcg accttgcggc ccaccagctt
     4261 gtttcgctcc aggatgacga cgtgcaagtc gattcacgtt agcacgcgtt ttcttgcgga
     4321 aaaccttttg tgcaaaagga cttcgatggc cttcggtaat cgtcgtcagc aaccgtacgt
     4381 tgaaggtcgg gtgctgggat ctgaggtggg agaacggtct ctgctacgat catgttcgtg
     4441 agggcactgg gcgcggcgat cgcgctgatg ttgatctcgc ttcctgctgg tgcggaggag
     4501 gcgaaacgcg aaacgacggg cgacaaacca ggcgagctgt accaaaccgt cgcgacgaag
     4561 aagtcgcgtg cggatctctc gcaattcctc gtcaaatccg cggagaaggc tgcgaaagcc
     4621 cgcgattggg caaaggcgat tccgctctat caagcgctcg ccgtcgcgcg aggtccggga
     4681 agtcccgagc agaagcagct cgctaccctg tggaacctcg ccggtcagaa cgaggaagcg
     4741 gccgaggcgt gggcgaccta cgcgaacgcg gcgaccgact ccaaggagaa agagcaggca
     4801 ctcaccgagg cgacgcgcct ctcgagcacg cccgacccgt ttgccgagaa actctcagtc
     4861 gccgcgctcg aaaaagacgc gaagcaggcg ttcaacctcg gacgcgcggc gttcaaagcc
     4921 aagaagtacg gcgacgcgct cgtgtacttc cacatgggct acgcgctcgc gccggatctg
     4981 ccgggcttcc ttcgcgagct cggctcgacg tacgacaagc tcggcgcgac cgatcgcaag
     5041 cgcgagttct atcggcgcta tctcgtgcag cgcccgttcg gtgcaaacgc tgacgtcgtt
     5101 cgatcggagc tcgcgcgcga caaggatgtg ctcggcacgc tgctcgtgtc gtcgagcctg
     5161 ccgtgcgccg agctctgggt gaaccgccag aagatcagcg gcaagatccc ggacaagggc
     5221 ctgctcgtcg cgcccggcaa ctacaaggga ctgtgcttca actccaagta cgagatggca
     5281 ctgttcgagt acgcggacgt cgagccgggt aagcaggcag cgatgtcgtt tcgctgggcg
     5341 atcatcgtga acaagctcga gcggccctat ggacgcattg cgctcgagaa tccgaagtcg
     5401 cccggaacga tgatcgacct cgggatcact tcgccggaaa tcggcgttgc ggtgcctcca
     5461 gacggccgca agctcaagat ggtcctcaaa gacgattcag gggtcagaac cgaagaacga
     5521 acggtcacca tcgagcctgg ccaaaagctc atcgtgcagt ggtaaggtcc cggcggcgtg
     5581 gtttccgagc ggccctcaca agaaggcaag ctatcgccgg gcgcaccgaa tgatccggtg
     5641 ccgttcggca agtggtactt gcttggcctg atcgcacgcg gcggaatggc cgaggtgtat
     5701 cgggcgcggc cgcaggatgg cgcgaagaat ttgtttgcgg tcaaagtgat gcgaccgcag
     5761 ctcgcccgcg aagctcgctt catcgacatg ttcaatcgcg agggaaagct cgcgatgatg
     5821 ctgaagaacc gctgcatcgt ggagaccgtc gagatcggcc aggatgacgg tcgccactac
     5881 atcgcgatgg agtacgtcgc tggccgcgac ctgacgcaag tgctccgccg ctgccaggaa
     5941 acgcagcagc gcattccggt tccgcacgcg gtctacatcg cggcgcgcat cgccgagggt
     6001 ctgcacttcg cgcacacgct ggtcggcccc gacggccgca cgctcaacat cgtcaaccgc
     6061 gacgtctcgc cgtcgaacgt gcggctctcg tatgacggtg acgtgaagct cctcgacttc
     6121 ggcatcgcgc aagcgctcat gaagttcacg agcgagatcg gcatcctcaa gggcaagttc
     6181 agctacatgt cgccggagca gatccgcggt atgccgctcg acgcgcggac cgacgtgttc
     6241 tccgctggca tcatcctcca cgagatgctc acgacggaga agctgttccg cggtgacacc
     6301 gagttcgcgc tgatggagaa ggtgcgcaag gccgaggtgc cgccgccgtc gaacttcaat
     6361 cgtcgcgtca cgcccgagct cgatgcgatc agcctcaagg ctctcgcgcg cgatgtcgcc
     6421 gatcgctatc aatccgctgc gcagctcgcc gcggatctcg acgcgctcat cgccggctac
     6481 cgcttcgatc cgaaggagct gcgtcagttc atgcgccagc tcttccgtaa ggaatacgcg
     6541 agagagctcg aagagacgga gaccgcactc gaggccgagc ccggcgccag cttcggcatc
     6601 ccgaactacg cgcagcgcgg cccgacgacg ggctcgatgc agagccaaga gacgccgacg
     6661 tccgaaatgc aaccgattcc gcgcccgggc ccgcaagcgc agatggcgga agacccgccg
     6721 tccgtcgaga tccagcagca cgaagagaag ccgaagggat tctggggctc tctcttcaag
     6781 cgcaagaagt agctcgcgtc gcgcccgcgt gcgctacgct ctggggcatg gcagccgtcc
     6841 cgcccgcacc gcagcgtccc gccaacgccg aagcgcgcat tgccggccac gcgatcggcg
     6901 tgctcgccgg agtcgcatcg ttgatcggcg gcgtccaagc gctgacgcat cacatgccgc
     6961 ccgtcctcgg cgtgacgatg ctcttgatga gcgctctctt gctcgcgctc acggcgttgt
     7021 cgtttcagca ctactcgcgg atcgcgtggt cgtttctcag cgcgatcctc gtcgtgatgg
     7081 ctctcgtcac gctgttcggc tcgccgaaag tccgcaacct cgtgcacatc ccgctcggtg
     7141 cagcgatggt gatcccgctc gtgctgatcg tcggtgtgat cgcgctctat ctcagcgccg
     7201 acgactaccg cggcacggtg tcccagaagt cgcagaacgc cgcgcgataa cccgcgccct
     7261 gcgagaccgg cacgtcgagc acctgaaccg gatcgctcgc cgcctcgtat ttcggccagc
     7321 cgccgccggg ctcgccggtc ttcgcaaacg atgtccatgc gctctgcatt gcgtcggcga
     7381 gtgacgtcgc gctgccgacc ttgccgagcg gatacgtctc gattccaaac acgaacggaa
     7441 tctccgcgga gtgaaacgcg tgcagctctg gaaacagtgc ctgctccaag tcgcgctgaa
     7501 acacgtagcg atacaccggc gagccagccg ccgtgagcgc gcgtgcgttg cgccgcgccg
     7561 gacacgtgaa gaacacgtcg cccgagacgg ccgcgagtgc ttcgttcggc gacggataat
     7621 cgccaacggg atagcgcgcg acgatctgat cgacgacggc cgcatcgttg aaccgccgca
     7681 tgagcgcttc gcgatactgc gtctcgtcgc gcacgaggtt cgacaagatg ttcgagtgaa
     7741 agagcgtgcc ctcgtccttg ttggtgccga ggatgatcgg ccgcttcgga tagtcgccgg
     7801 ccgagagccg cttctcgatc tgctcgggaa tcacgacgcc gtcgacattg ggcagcgtgt
     7861 tcggcggata cgagtcatag aagaagccgc ctggcttctg catcatgatg ggcgcgagcg
     7921 ccgtcgcgtc gagcaccgca taatcgatga ggtcgcgcat gcacgcgatc gcaccggcat
     7981 cgctgcccgg gcagcccact ttcgcgccga acgcgagccc gtcgctctcg gcctgctgcc
     8041 gcgtctgcgc gagcccgtta tcgaggcagg cgccgctctc gacgatcgcg cgctcgaaca
     8101 agcccgccgt tttcggatcg gtgtagtgca cgcagcccga gaaacctccc gccgactcgc
     8161 caaagatcgt gacgtgtccc gggtcgccgc caaactcgcc gatgttgcgc tggacccact
     8221 cgagcgcagc gagctgatcg cggaggccgt aattccccga cgtgtgatcc gggtcctccg
     8281 ccgcaagcgc tggatgcgcg agaaagccga ggccgccgag ccggtaattg atcgtgacga
     8341 cgacgacgcc gcgcgtgcgc gcgagctccg cgcccgaata aaacggatcg ccgccgctgc
     8401 caaagacaaa cgcgccgccg tgaatccaga acatcacggg cagcttcccc gtcgccgccg
     8461 acggcgccca cacgttcagc gtcaagcagt cctcgatccc gccgccgctc gtgatgacgg
     8521 tgttctgcgg gcacttctgt ccgactttcg taccgtcgcg cgcgccgtcc cacgcctctg
     8581 cccgcttcgg cggcttccac cgcagctctc cgacgggcgg tgccgcatac ggaattccga
     8641 gccagctctt cacgcccgcg ccgtcgtcgc tgccacgcac gacgccggtc tcgacctcga
     8701 cgtcgttcgg tccaagctcg tcgccgcacg ccgccgcgcc gccgac
//
LOCUS       DATLBZ010000389         6063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4163220, whole genome shotgun sequence.
ACCESSION   DATLBZ010000389 DATLBZ010000000
VERSION     DATLBZ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4163220"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VMZ53_19775"
     CDS             <1..376
                     /locus_tag="VMZ53_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30764.1"
                     /translation="LNAAITKRLGLRTRRRGSVFTDRYHVTIIRTPRHARHELAYVLN
                     NWRRHGESTRRLSDGWRVDPFSSAPSFDGWRDVEALEQTWPHSYVPLPVWKPRTWLLS
                     EGWRRYGLIKSTEVPGPRWSPR"
     gene            complement(676..1311)
                     /locus_tag="VMZ53_19780"
     CDS             complement(676..1311)
                     /locus_tag="VMZ53_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30765.1"
                     /translation="MSVWPITRGPAFHSYADTRSWLGIPNTLDVVSNVAFVIAALWMW
                     RRTRGLARLACAGTAAIGIGSALYHWSPSDTTLAFDWAPIATTLMWVLATVVEDRGGP
                     GRAVAIGGALAAIGSVVYWLATGGTHGGDMTAYVVVQGAGVALPPLVALFRQGRIPAL
                     QLVLALGCFGLARLAAAHDRDLLDAIGISGHSLKHVIAALAAAIVLRAMRR"
     gene            complement(1318..2226)
                     /locus_tag="VMZ53_19785"
     CDS             complement(1318..2226)
                     /locus_tag="VMZ53_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30766.1"
                     /translation="MVTKALFILAVVVCGLAGTAAAQAPQKITIGIYAPTVEFGTAQA
                     RLAYVQGLAKAIEQNTGIKTEAQSYASIAALKKDNVDFAIIDGPCYATNLSWKLLANA
                     NIGGGTTRQWALFSSAGDTMQALKGKKLAFVAMGCNDNGFIDNAMLESEVDDSFFGAR
                     VGEKELTGALASVTSYKTAQAVFAPTASAKGLSKVFETGAVPNPAFVEVAGKLPAGTS
                     DKVAAAVVGYGGSGAINGWTKATKDVYQSFAGRLSPVKKAGVFASPEPVRIDAKDVLV
                     DPATLKDSGMVAIRHHFVRPPGSRMD"
     gene            complement(2216..4681)
                     /locus_tag="VMZ53_19790"
     CDS             complement(2216..4681)
                     /locus_tag="VMZ53_19790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS30767.1"
                     /translation="MQAAVEDDLRDGDKYFEDGDWKKAAAAYDRAIDKAPSQVAAEAY
                     GKRAAIYIILKDYKGGLDFIAKAKTRYPNAPEILEQEALILWESGQHDEAIKVAEKVV
                     KAKPSSFTNQKLIGEYYAQRDPVKTATAFEAYLSNRPPELEGGDVLPRVRLGFAYIAN
                     ARDVLGDGDEDRAGALYKKAAEQFEYVQRKLGKKPYAAVNAENGLCAAYTGMARWDNA
                     INVCEKAIQDPKHVDSAGSAWFNLGRAYLARKQTKKARSAGNEFTRLRKNEARGYILL
                     GETYFEDRDWGNALDQFSRAEKALKPNQAREQVQLSIWLGKTYRRLPAPTSGNNPNLN
                     LAIEKLSTGYSANPSSIELAAELGGAYLEAKQDAKATALTDKLLAGPDLAKAPPEQRA
                     AVLVISGKSLFNQKKLKEARQRFEAARQIRQNDIQVQRYLVTTINEQAFLEKDAKASQ
                     ALLSEALAIDGSSPVTLTNVAVLAIDRGDCDGAQKQLLKLKDVRGSDAVVTARLLART
                     YLCGNKPDVRKASEAYGAAEREAKKANAQLALAEIYTEWAPLLWDSDVVSAVDKLEQA
                     VQIAGQDADIAPAAKRNLALGLYRRGWKSMREGRAGEASTDFDRALRDPSVLKGTEPL
                     AFEFSAAVAALDAGRSADAAKLFRTLANKGNQGSYLKGPYAKVGSTFFAAYASYRSAT
                     GKAREAACNDLARLEGDLGNKARELSASCWELAAVEDWRSGNWGQAQKDIASADRSAN
                     ADQKRRLVNDRAALSLGKDKLNELEGLAGNPAEALVNLGIVYDMIGKPKEAYDAWVRA
                     KGKGVNARDLQKWIDAKKRIYGY"
     gene            complement(4726..>6063)
                     /locus_tag="VMZ53_19795"
     CDS             complement(4726..>6063)
                     /locus_tag="VMZ53_19795"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HUS30768.1"
                     /translation="GTQLDCKKLDPHVPVSATLGPATVDSWQKVQAAWEAAAKSNGNP
                     LVDLTIASGGANYLIFAYPIFVGEPATAAGAAAADPPQARQGYVVLGYDLEPVAWYKA
                     EANEQKAAAVKKAALYTGAVGALFALIGTLLAIFQGLSISKPLKELAWKADQIARGDL
                     DARVEVTSGDEIGLLGENFNFMADQIAILLQQTAEKAKIEQELEVARAIQETLVPSDA
                     PVKKSTLTFAGFYQPAAQTGGDWWTWAELAGGKILVVIGDVTGHGVPSAMITAAAKAS
                     CDVARHVHNDDVTVTTLLEIMNHAIYESAQRRFVMTCFASIVDLQKRTITYANAGHNF
                     PYLFRPGEGKGEFGSLMIRGNRLGDDRNSKYESKTTELAIGDVLVWYTDGIVECENDT
                     GEEYGEKRFRASVRKAAALDAGEMRDAIITEAGAYFGDTARKDDITMVVGRIH"
BASE COUNT          943 a         1989 c         1973 g         1158 t
ORIGIN      
        1 cttgaacgcc gcgatcacga agcgcctcgg cctgcgcacg cgtcgccgcg ggtcggtgtt
       61 taccgaccgc taccatgtaa cgatcatcag gacgcccagg catgctcgcc acgagctcgc
      121 gtacgtgctc aacaactggc gtcggcacgg tgagagcacg aggcgcctct ccgacggctg
      181 gcgcgttgat ccgttctcga gcgcgccgag cttcgatggt tggcgcgatg tcgaagccct
      241 cgagcagacg tggccgcatt cttatgtgcc gttgcccgta tggaaaccgc gcacgtggtt
      301 actttccgag gggtggcggc gctatggcct gatcaagtca acggaagtgc cgggcccgcg
      361 ctggtcgccg cgctgagctg agcccttatt ttgcgtcgac agcgggctga gctgagcagc
      421 cttttcctcg aaggaagaat tgaaacagaa gtgccacgcg ctctgatgca gcaacgagga
      481 gatggacgtg tgggtcgcgg catcgcgctg aagggcggag cgcttcgaga ccagccggtc
      541 gcgcgccgcg cgacggggcg cttcgagacc agccggtcac gctccgcagc tgttgttgga
      601 ctgcgtgcgc agcttcgccg cgtgtgcgat cgacatcgtc catcgcgcga cgccgcgcaa
      661 gctctggccg agcggctagc ggcgcatcgc gcgcagcacg atcgcagcgg caagcgcagc
      721 gatcacgtgc ttgagcgagt ggcccgagat gccgatcgcg tcgagcagat cgcgatcgtg
      781 cgcggcggcg aggcgagcga ggccgaagca gccgagcgcg agcacgagct ggagggcagg
      841 gatgcggccc tggcgaaaca gcgcgacgag cggtgggagc gcgacgcctg caccttgcac
      901 gacgacgtac gccgtcatgt cgccgccatg cgtgccgccc gtcgcgagcc agtagacgac
      961 gctgccgatc gctgcgagcg caccaccgat cgcaaccgcg cgaccgggtc cgccgcgatc
     1021 ttcgacgacc gtcgcgagca cccacatgag cgtcgtcgcg atcggtgccc agtcgaacgc
     1081 gagcgtggtg tcgctcggtg accagtgata cagcgccgag ccgatgccga tcgcggcggt
     1141 gcctgcgcat gcgagccgcg cgaggccacg cgtgcggcgc cacatccaga gggcggcgat
     1201 gacgaacgcg acgttgctga cgacgtcgag cgtgtttgga atgccgagcc acgagcgcgt
     1261 gtcggcgtag ctgtgaaacg ccgggccgcg cgtgatcggc cagacgctca ttgcttgcta
     1321 gtccatgcgc gagccgggcg ggcgcacgaa gtggtggcgg atggcgacca tgcccgaatc
     1381 cttcagcgtc gccggatcga cgagcacatc cttcgcgtcg atgcgaaccg gctcggggct
     1441 cgcgaacacg ccagccttct tcaccggact gaggcggccc gcgaacgatt gatagacgtc
     1501 cttggtcgcc ttcgtccaac cgttgatcgc acccgagccg ccgtagccga cgaccgctgc
     1561 ggcgaccttg tcactcgtgc cggcggggag cttgccggcc acctcgacga acgccgggtt
     1621 tggaaccgcg cccgtctcga aaactttcga gaggcccttc gcactcgcgg tcggcgcgaa
     1681 caccgcctgc gccgtcttgt acgacgtcac cgatgcgagc gcaccggtca gctccttctc
     1741 gccgacacgt gcaccgaaga agctatcgtc gacttccgac tcgagcatcg cgttgtcgat
     1801 gaagccattg tcgttgcagc ccatcgcgac gaacgcgagc ttcttgccct tgagcgcctg
     1861 catcgtgtcg cctgcgctcg agaacaatgc ccattggcgc gtcgtgccgc cgccgatgtt
     1921 cgcgttcgcg agcagcttcc agctgaggtt cgtcgcgtag caaggcccgt cgatgatcgc
     1981 gaagtcgacg ttgtccttct tgagcgccgc gatcgacgcg tacgactgcg cctcggtctt
     2041 gatgccggtg ttctgctcga tcgcttttgc gagcccttgc acgtatgcga ggcgcgcttg
     2101 cgcggtgccg aactcgacag ttggtgcgta gatgccgatc gtgatcttct gcggcgcttg
     2161 cgcggccgcg gtgcccgcga ggccacaaac gaccaccgcg aggatgaaga gggccttagt
     2221 aaccataaat cctcttcttc gcgtcgatcc atttctggag gtcgcgagcg ttgacgccct
     2281 tgcccttcgc gcgcacccac gcgtcgtagg cttctttggg cttaccgatc atgtcgtaga
     2341 cgataccgag gttgacgagc gcctcggcag gattgccggc gagcccttcg agctcgttga
     2401 gcttgtcttt accgagcgag agcgccgcgc ggtcattaac gaggcgacgc ttttgatccg
     2461 cgttggccga gcgatccgct gacgcgatgt ctttctgcgc ctgcccccag ttgcccgagc
     2521 gccaatcctc gacggccgct agctcccagc acgatgctga tagctcgcgc gccttgttgc
     2581 cgaggtcgcc ttcgagccga gcgaggtcat tacatgcagc ctcgcgcgcc ttgccggtcg
     2641 cactgcggta gctcgcgtac gcggcgaaga acgtgctgcc gaccttcgcg taggggccct
     2701 tcaggtacga gccttggttg cctttgttcg cgagcgtgcg gaacagcttc gcggcatcgg
     2761 cgctgcggcc agcgtcgagt gcggcgactg cggcggagaa ctcgaatgcg agcggctcgg
     2821 ttcctttgag caccgacggg tcgcgcagcg cgcgatcgaa atccgtcgac gcttcgccgg
     2881 ctcgaccctc tcgcatcgac ttccaaccgc ggcgatacag gccgagcgcc aagttgcgct
     2941 tcgcggccgg cgcgatgtcg gcatcctgac cggcgatctg cacggcttgc tccagcttgt
     3001 cgacggcgga cacgacgtca ctgtcccaga gcaacggcgc ccactccgta tagatctcgg
     3061 cgagagcgag ctgcgcgttg gccttcttcg cttcgcgctc ggcggcgccg taggcctcgc
     3121 tcgccttgcg cacgtcgggc ttgttgccgc agaggtacgt gcgagcgagc aagcgcgcgg
     3181 tgacgacggc atcactgccg cgcacatcct tcagcttgag cagctgcttt tgcgcaccgt
     3241 cacaatcgcc tcggtcgatc gcgagcaccg cgacgttcgt cagcgtgacc ggcgagctgc
     3301 catcgatcgc gagtgcctcg ctcagcagcg cctgcgacgc cttcgcgtcc ttctcgagga
     3361 aggcttgctc gttgatcgtc gtgacgaggt aacgctggac ttggatgtcg ttctggcgaa
     3421 tctggcgcgc ggcttcgaag cgctggcgcg cctctttcag cttcttctga ttgaagagcg
     3481 acttgccgga gatcacgagc accgcggcgc gctgctcggg cggagccttc gcgaggtccg
     3541 gaccagcgag cagcttgtcg gtgagcgcgg tcgccttcgc atcctgcttc gcctcgaggt
     3601 atgcaccgcc gagctctgcg gcgagctcga tgctgctcgg gttcgcgctg tatcccgtcg
     3661 agagcttctc gatcgcgagg ttcaagttcg ggttgttgcc ggacgtcgga gcgggcaggc
     3721 ggcgatacgt cttgccgagc cagatcgaga gctgcacttg ctcgcgcgct tggtttggct
     3781 tgagcgcctt ctcggcgcgg gagaattgat cgagcgcgtt gccccaatcg cgatcctcga
     3841 agtacgtctc accgaggagg atgtagccgc gcgcttcgtt cttgcgcagg cgtgtgaact
     3901 cgttgcctgc agagcgcgcc ttcttcgtct gcttacgagc gagatacgct cggccgaggt
     3961 tgaaccacgc cgagcccgcc gaatcgacgt gcttgggatc ctggatcgct ttctcgcaga
     4021 cgttgatcgc gttgtcccag cgcgccatgc ccgtgtatgc cgcgcacaga ccgttctcgg
     4081 cgttcaccgc ggcgtacggc ttcttgccga gcttgcgctg cacgtactcg aactgctcgg
     4141 cggccttctt gtagagcgca ccggcgcgat cttcgtcgcc atcgccgagc acgtcgcgcg
     4201 cattcgcgat gtacgcaaag ccgaggcgga cacgaggcag cacgtcgccg ccttcgagct
     4261 cgggtgggcg attcgagaga tacgcctcga acgcggtcgc ggtcttcacc ggatcgcgct
     4321 gcgcgtagta ctcgccgatc agcttctgat tcgtgaacga gctcggcttc gccttgacga
     4381 ctttctcggc gaccttgatc gcttcgtcgt gctggccgct ctcccacagg atgagcgctt
     4441 cctgctcgag gatctcgggc gcgttcggat accgcgtctt cgccttcgcg atgaagtcga
     4501 gaccgccctt gtagtccttg aggatgatgt agatcgccgc gcgcttgcca tacgcctcgg
     4561 cggcgacttg gctcggagcc ttgtcgatcg cgcggtcgta ggcagcggcg gctttcttcc
     4621 agtcgccatc ctcgaagtac ttgtcgccgt cgcggaggtc gtcttcgacg gccgcctgca
     4681 catagggagc gacgatacag atcgtcgctg ctgctaagag tgccctcaat ggatcctccc
     4741 gacgaccatc gtgatgtcgt ctttgcgtgc cgtgtcaccg aagtacgcac ccgcctcggt
     4801 gatgatggcg tcgcgcatct cgcccgcatc gagcgcagcg gccttacgga ccgatgcgcg
     4861 gaaccgtttc tcgccgtact cctcgccagt gtcgttctcg cactcgacga tgccgtccgt
     4921 gtaccagacg agcacgtcgc cgatcgcgag ctcggtcgtc ttcgattcgt acttcgagtt
     4981 gcgatcgtcg ccgaggcgat taccgcgaat catcagcgag ccgaactcgc ctttgccttc
     5041 acccggacgg aacaggtacg ggaagttgtg gccggcgttc gcgtacgtga tcgtccgctt
     5101 ctgcagatcg acgatcgacg cgaagcacgt catcacgaac cggcgctgag cgctctcgta
     5161 aatcgcatgg ttcatgatct cgagcagcgt cgtgaccgtg acgtcatcgt tgtggacgtg
     5221 gcgcgcgacg tcgcatgacg ccttcgcagc ggcggtgatc atcgcggacg gaacgccgtg
     5281 acccgtgacg tcgccgatca cgacgaggat cttgccgccg gcgagctcgg cccacgtcca
     5341 ccagtcaccg cccgtctgtg cggcaggttg gtagaagccc gcgaacgtca gcgtgctctt
     5401 cttcaccggg gcgtccgacg gcaccagcgt ctcttgaatc gctctggcga cctcgagctc
     5461 ctgctcgatc ttcgccttct ccgccgtctg ctgcagcaag atcgcgatct ggtcggccat
     5521 gaagttgaag ttctcgccga gcaatccgat ctcgtcgccg ctcgtcacct cgacgcgagc
     5581 atcgaggtcg ccgcgggcga tctggtcggc cttccacgcg agctccttga gcggcttcga
     5641 gatcgagaga ccctggaaga tcgcgagcag cgtaccgatg agcgcgaaca gcgcgccgac
     5701 agcgccggtg tagagcgcgg ctttcttcac ggctgccgcc ttctgctcgt ttgcttcagc
     5761 cttgtaccaa gcgaccggct cgaggtcgta accgaggacg acgtagccct ggcgcgcttg
     5821 cgggggatcg gcagcggcag cgcctgcagc ggtcgcgggc tcaccgacga agatcggata
     5881 cgcgaagatg aggtaattcg cgccgcccga cgcgatcgtc agatcgacga gcgggttgcc
     5941 gttggacttc gcggcggctt cccacgcagc ttgcactttc tgccacgagt cgacggttgc
     6001 aggacccagc gtggcggaca ccggcacgtg agggtcgagc ttcttgcaat cgagttgcgt
     6061 tcc
//
LOCUS       DATLBZ010000390        13032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1948615, whole genome shotgun sequence.
ACCESSION   DATLBZ010000390 DATLBZ010000000
VERSION     DATLBZ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13032
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948615"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VMZ53_19800"
     CDS             <1..877
                     /locus_tag="VMZ53_19800"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUS30769.1"
                     /translation="FEVAADDEGLRLDQVIPKHIAGLSRRKTRAVIDLGGVFVDRTRV
                     RVAGRLVKRGQVIEVNVGGAIERANETPLAPRIVHVDDHIIVIDKPAGLVTAPTPESN
                     RGDALDQLGEQFGEVYLVHRLDLPTSGLLVFARTRDANKILGEAFKVHDVDREYRAVV
                     VDGVAAQTIDREIEGRRAVTHVEPVETFARITPDGTLTGETHATLLRARLETGRTHQI
                     RLHLAGLGHPVCGDRTHGGEKERAFLPRPPRMALHAMVLGFAHPATNERVRWEAPLPD
                     DLATWIARLRPRTPT"
     gene            complement(874..1161)
                     /locus_tag="VMZ53_19805"
     CDS             complement(874..1161)
                     /locus_tag="VMZ53_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30770.1"
                     /translation="MVQRFTFVKLLDDEVGGRSELAEKLRAELVAAGADAVVGLPADD
                     SAVRWDMSIVITAASLDAWNALSKQPAMVGVFDELAEKAAVVKAWTFSAVC"
     gene            1277..1783
                     /locus_tag="VMZ53_19810"
     CDS             1277..1783
                     /locus_tag="VMZ53_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30771.1"
                     /translation="MHRLALLATLAAAACTSEPSTDTGQLVRVHDQVFAECQDTADAT
                     CSVEVTFTNGQLSALGVYEDGAVTGPVAIATLSESARARLADLIAQIPLESPDTVHDV
                     GCGLAPLRTTNADIAFDHDGMRNFVIEYAAQGPMADFSRYVTDLVTGIRTCNSSDLAF
                     ESCQPNVF"
     gene            complement(1780..2658)
                     /locus_tag="VMZ53_19815"
     CDS             complement(1780..2658)
                     /locus_tag="VMZ53_19815"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUS30772.1"
                     /translation="MERQAAPTTTTTRALLDGYDGILLDLYGVLVDAGGLLPRAREFL
                     AELDARRKPFAFVSNDASRSTATYVERFASWGVQVAPERFVTSGSLLPAYFAERKLAG
                     ARVAVLGTADSEAFVREGGGDVVAVRPGMEIDAVVVADDAGFAFLDGLEASLSAIVRA
                     VEAGRRPALVLPNPDLVYPKGGGELGFTAGTMALMIEAALERRFPGAGLVFDRLGKPE
                     PHLFAAAAKQLGIAVDRLVMVGDQLETDIAGANAAAVPCALLAGAISRWTNEESAHRA
                     PRARARPTWLLDALWP"
     gene            2707..3516
                     /locus_tag="VMZ53_19820"
     CDS             2707..3516
                     /locus_tag="VMZ53_19820"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HUS30773.1"
                     /translation="MRFAIVLAGLLVVGAACSDSHPMSPAALPTPSRPRIIAHRGAST
                     DETENTIAAFKRAWDLGVEGVELDVRVSRDGQVIVMHDDNTKRLTGVDKLVAEQTLAE
                     LKKLELPKKQRIPTLREAMATIPPGRAMFVEIKTGIETVPAVAAAIRASWPSNTTIML
                     QGFDADTLVALATAVPDAPAYWTVDPPLDEANQPLPYPREVIDEAVRRKFAGLALLAP
                     SVDDNLLAAARDAGILIDVWTLNDVPSLSAWAGREVRWMETDRPDLAPMQP"
     gene            3551..4348
                     /locus_tag="VMZ53_19825"
     CDS             3551..4348
                     /locus_tag="VMZ53_19825"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HUS30774.1"
                     /translation="MRAYLDLLRTVLEHGERRTDRTGTGTISMFGAQTRYDLREGFPL
                     VTTKKVLFPAVVRELLWFLRGSTNINDDLKQHTPIWDAWADENGELGPIYGYQWRHWG
                     ASPQNPGGIDQIQEAIDTIKRDPTSRRIIVSAWNVADIPKMKLPPCHAFFQFYVNDKW
                     LDCQLYQRSADLALGVPFNIASYALLMSMIAQECGLSPRHFVHTLGDAHIYLNHVEGV
                     KIQLSRAPLPPPTLVLAPGKKTLEYTFEDISLEGYQHHPFIKFPVAV"
     gene            4386..5378
                     /locus_tag="VMZ53_19830"
     CDS             4386..5378
                     /locus_tag="VMZ53_19830"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30775.1"
                     /translation="MKPGTIIAGRYEILAKLGAGGMGEVFRARHRSLDKEVALKLLVA
                     GSADFEVRFEREAKAAARLDHPNCVRVIDYGKSGNYHFIAMDLLVGETLAAALSRGPM
                     TIERTVHITKGLLSALDHAHSHGVLHRDLKPENVFLVGGRPVLIDFGLATLAGHTRVT
                     GEGMCMGSPSYVAPERLIGQPADARTDLYALGVMMYEMLAGTRPFTGPTPHETMKRAL
                     QMPPRPLAALRRDIPPHLDFVIRKALVKDPKRRFATAIDMLEELAEVPLYEFEDEPEM
                     LLERVDEASTTALVTFRKPSFFARLWSWFRYGAWRWARLATARAAPTSARLSVA"
     gene            complement(5381..6133)
                     /locus_tag="VMZ53_19835"
     CDS             complement(5381..6133)
                     /locus_tag="VMZ53_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30776.1"
                     /translation="MAKLIPFALLAACDAGALPPKPEPTKKLVEVPAPSDAAVVVPAD
                     AASPTPPLAGVSLQMNHFDLPPQAECNRTRLVLRLIDKTKKVYDEYKENSTCTGACTD
                     KKKREGAAEVAELEKQIEKGETPSVLDYNFTSCLETGLAETQIIRKVGGRDVAIITDR
                     YIGPHDTVPAGFKVAFEVCGKLFVSDRFADVEFMSWRLDQLRVTSEDGSIFVQGIGDD
                     GTAAEHTGTLLRVQLPACPAAPVVQTFDIWPH"
     gene            complement(6169..6699)
                     /locus_tag="VMZ53_19840"
     CDS             complement(6169..6699)
                     /locus_tag="VMZ53_19840"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HUS30777.1"
                     /translation="MSFDVVVAADLDWGIGKANALPWPKLKGDLAHFRRVTSACSEGK
                     INAIIMGRKTWESEEVKGSPLPRRLNVVVTRKQLTVPEGVVVAASVDAAIHAATAPNV
                     ETIFVVGGAEIFKDAFARADLRYVYVTRIEGHFETEVRIPNLDELGFVRTDWDGEQAA
                     EENGVRYKIERLARRV"
     gene            6728..7000
                     /locus_tag="VMZ53_19845"
     CDS             6728..7000
                     /locus_tag="VMZ53_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HUS30778.1"
                     /translation="MATVVFTPNLKRHVECPTEEVVGATVRAVLDGVFAHNPKLRGYV
                     LDDQGALRRHMIVFVDGSQIDDRDNLSDPVRPTSEVYVMQALSGGS"
     gene            7000..8112
                     /locus_tag="VMZ53_19850"
     CDS             7000..8112
                     /locus_tag="VMZ53_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HUS30779.1"
                     /translation="MTAQSILVSTRKGLFVAEPAGSSYKVTRGAFVGDNVSISMVDPR
                     DGAWYAALDHGHFGVKLHRSDDKGASWTEITTPAYPPKPEGQVEKTMWGKDRAWSTLL
                     IWAMAPAPEKAGALWAGTVPGGLFRSDDRGQSWRLNEALWFHPDRPKWNGVVKEEAGI
                     HSICVDPRDPKIISVGVSTGGVWRTKDGGATWTQSAHGMRSEYVPPELTRDGGNQDVH
                     CLVQCAADPKTFWVQHHNGIFRSNDDMATWTELSENARPSQFGFPVVCDPKDPNTAWF
                     VPAQKDEKRIPVEGKVVVSRTRDGGKSFEVLSKGLPDTFAYDLTYRHALALSTDGALA
                     FGSTTGNVFASRDRGESWSVVSNYLPPVHAVTFADV"
     gene            8162..9226
                     /locus_tag="VMZ53_19855"
     CDS             8162..9226
                     /locus_tag="VMZ53_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30780.1"
                     /translation="MKYLLVIALLTACGSHIKNGGDDDDVDSGLGSNGNFPDCDPSTF
                     GGMTCNGNAVVSCNADGTFGQTVTTCGNGMECRDGSCKNACTADGVDLVYVVDEMNDF
                     MSFDPRLLPGDPFHKIGTLSCPHNNTSIQTGNNVAMPFSMSVDRDGVAWVLYTTGELF
                     KVSLQDASCTKANNTIGASSMFLFGMGFALDAPMVDTEKLFLAGGNRDPSTTPRRLGW
                     DDTHGNNLTPTNVGNIAAASDYSPELTGTAEAKLYGFYPRIAQVPFVQEIDKSTGAAV
                     GQTWNVGTTGLGTGIRDWAFAQWGGVFYVFITTETNGVRNSTVRSVDRASSAYKVELQ
                     NLPYYIDGAGVSTCAPAVLQ"
     gene            9286..>13032
                     /locus_tag="VMZ53_19860"
     CDS             9286..>13032
                     /locus_tag="VMZ53_19860"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HUS30781.1"
                     /translation="MRNIGWILVAAFVICLGACGGKKSGPGGLTVTGSPTGQIDGEVR
                     VILAFSKPMVAKDRIGQVATTAPLSLVPDIAHEMRWTDDKTLVAVPTASLPISTKFTV
                     TVPADAKALDGSTLGKATSFEFFTERLSLTTEVVGSKDRAAKNQLVKLTFNHEVAFDQ
                     IEKLCSFTSKSGSQKAKLAPDSNAGPAKQYLLVPAADLALDTDWALACKAELKGVVGN
                     LGLEGPFEEKFHTYGALKYVDREPKDNDIVPDENLKIKLVFSNPLAEPFKLKIAPAVA
                     GFPQGCHAMTDDPVGVSCSGAFEALTEYTITVDAEQKDIFGQKLGKVEQVDFRTTDAK
                     PTISMEDGYFIGELKRPVVPLWARNVTKLDVTVVPVTQDNFHEIIPLLDWWEHTPADF
                     KKTHLKPVAKQIAVSGQKNKWVQSSIDPATLLGGQPGPGMYYVEIGSPEVAKGAFVDG
                     GRKKVLVNFTNIGVVSKLSPTRGVVWATNLVTGKPLPGASVSVRDATGKVTWNGTTDN
                     EGVAVLPGNDKLGATESEYGGDHIDDLRIHVSQGPDWTMINPSASGGLSPWSFNVSVD
                     RSSGSTKLRGFMHTDRGLYRPGDKVHVKGLARVTKLGEPLAVPNTGKKVAVEVEGPQG
                     KSIMTTDAKLSAFGGFWFDIDLPGDARLGDYTIRAKLEHGTFTRTFSVEEFRAATFEV
                     NGKTKESLLVSRGDVHGTVSANYFYGAPVRNGSVDGNVYTRKRRVSFDQLKEFDFGDE
                     RSWESYYYYESNDSQSMLTDAHVSTDAKGNAAFSFPVSPDDVYSDADLLIRADVHSPS
                     NEVISKSFTIPYFRSKKYFGIKPPGYFLDVKKPAKFQVVSASPDGKIVDGSAKVKVTR
                     RDWNCVYEDWGYRGSYQCKDTTQTILEKTVAIAGGKPAEIEFTPATGGDYLVVVEGDK
                     DAAAAAQRLYAWGDDTGSWRSDDTMTFEVATDKQEYKTGDTATLILKTDLANATGLVT
                     IERDGVIEKRLIDITPTTKHLKIPITAAMAPNVYVSVALVQGRTGDGPRGKPRMRMGV
                     ANLNVRPEDNKLTVSVEPDKKEYRPGEQVTATVKVTDSKGQPVSAEVSITAADEGVLS
                     LIAYQTPDPIPTMFAPWGLGVTSATQLEYIRDIPAPNVERPATGGDSAGSVRSRFVAS
                     AVWVPGAVTDANGVATVTFKAPDNLTAFRLMAVAADKGYRFGSADKRFAVNKPLQLHS
                     ALPRFVQLGDAAKAGVVVHNETGKAGQATVKVVGDEHLAIKGATE"
BASE COUNT         2368 a         4237 c         4250 g         2177 t
ORIGIN      
        1 gttcgaagtt gccgccgacg acgaaggcct gcgcctggat caagtcattc ccaagcacat
       61 cgctggtctg tcgcgccgca agacccgcgc agtgatcgat ctcggtggcg tgttcgtcga
      121 tcgaacgcgc gtgagggttg ccggacgcct cgtcaagcgc gggcaagtca tcgaggtcaa
      181 cgtcggcggc gcgatcgaac gtgcgaacga gacgccactc gcgccacgca tcgtccacgt
      241 cgacgaccac atcatcgtca tcgacaagcc ggcgggtctc gtcaccgcac cgacacccga
      301 atcgaatcgc ggagacgcgc tcgatcagct cggcgagcaa ttcggggaag tctatctcgt
      361 ccaccggctc gatctaccga cgagcggtct gctcgtgttc gcgcgcacgc gcgatgcgaa
      421 caagatcctc ggtgaggcgt tcaaggtcca cgatgtcgat cgcgagtacc gcgcagtcgt
      481 cgtcgatgga gtcgcggcgc agacgatcga tcgcgagatc gaaggtcgtc gcgcggtcac
      541 gcatgtcgag ccggtcgaga cgttcgcccg gatcacgccc gacggcacgc tgaccggcga
      601 gacccacgcg acgctcctgc gcgctcgcct cgagaccggc cgtacgcatc agatccgtct
      661 gcaccttgcg ggactcggcc atccggtctg cggcgatcgc acgcacggcg gcgagaagga
      721 gcgggccttt ctcccgcgcc ctccgcgcat ggctctgcac gcgatggtgc tcggcttcgc
      781 gcatcccgcg acgaacgagc gcgtccgttg ggaagcgccg ctgcccgacg atctcgcgac
      841 gtggatcgcg cggctacggc cgcgaacgcc gacctagcag acggcgctga acgtccacgc
      901 tttgacgacg gcggccttct cggcgagctc gtcgaacacg ccgaccatgg cgggttgctt
      961 cgagagtgcg ttccacgcgt cgagcgacgc ggcggtgatc acgatcgaca tgtcccagcg
     1021 aacggcgctg tcgtccgcgg gaagcccgac cactgcatcc gcacccgcgg cgacgagctc
     1081 tgcccgcagc ttttcggcga gctcgcttcg tccgcccacc tcgtcgtcga ggagcttgac
     1141 gaatgtgaac cgctggacca tggcggcagc ctagctccgt cgtgcccagt tttcgaaaac
     1201 cgtcctcgac tttctgagag gctgccgctg gcaatcgcat agttgcgctg gcacacgccg
     1261 tgctgcatgc cgccccatgc atcgcctcgc gctccttgcc acgctcgctg ccgccgcctg
     1321 tacatccgag ccctccaccg acacgggcca gctcgttcgg gttcacgacc aagtcttcgc
     1381 cgagtgccag gacaccgcgg acgccacatg cagcgtcgag gtgacgttca cgaacggcca
     1441 gctgagcgcg cttggcgtct atgaagacgg agccgtcacc ggacccgtcg cgattgcaac
     1501 gctcagcgag tcagcgcggg ctcggctcgc ggatctcatc gcgcagattc cgctcgagtc
     1561 gcccgacacc gtgcacgacg tcggctgcgg cctcgcgccg cttcgcacca cgaatgcaga
     1621 catcgcattc gatcacgacg gcatgcgcaa cttcgtcatc gagtacgcgg cccaagggcc
     1681 gatggccgac ttctcgcgct acgtgacgga cctcgtcacg ggcattcgca cctgcaactc
     1741 ttcggaccta gcgttcgaga gctgccagcc gaacgtcttc taaggccaca gcgcgtcgag
     1801 cagccacgtc ggccgcgcgc gcgctcgggg cgcgcgatgt gcagattcct cgttggtcca
     1861 gcgcgagatt gcacctgcga gcagtgcgca tggaacagcc gcggcgttcg cgcctgcgat
     1921 gtcggtctcg agctgatcgc cgaccatcac gaggcgatcg acggcgatgc cgagctgctt
     1981 cgcggcggcc gcgaacaagt gcggctccgg cttgccgagg cgatcgaaca cgaggcccgc
     2041 accgggaaat cgtcgctcga gcgcggcctc gatcatgagc gccatcgtgc ccgcggtgaa
     2101 gccaagctcg ccgccgcctt tcggatagac gagatcggga ttcggcagca cgagcgcagg
     2161 ccggcgtccg gcttcgaccg cgcgaacgat cgcggagagc gatgcttcga gaccgtcgag
     2221 aaacgcgaag cccgcgtcgt cggcgacgac cacggcatcg atctccatgc ccggccgcac
     2281 ggcgacgacg tcgccaccgc cttcgcgcac gaacgcctcg gagtcagccg tcccgagaac
     2341 ggccacgcgc gcgccagcga gcttgcgctc tgcgaagtac gcggggagta gcgagcccga
     2401 ggtcacgaac cgctcgggcg ccacttgcac gccccacgat gcgaaccgct cgacataggt
     2461 cgcggtcgac cgcgatgcat cgttgctgac gaacgcgaac ggcttgcgtc gagcgtctag
     2521 ctccgcgagg aattcgcgcg ctctcggcaa cagaccgccc gcatcgacga ggacgccgta
     2581 caggtcgagc aggatcccgt cgtagccgtc gaggagcgcg cgtgtcgtcg tcgtcgttgg
     2641 cgcagcctgc cgctccatgc cgtgacttta cacgggcctt acgcagtcgc gagttaccct
     2701 tcgagcatgc ggttcgcgat cgttctggct ggcttgcttg tcgtcggagc agcatgctcg
     2761 gactcgcatc cgatgagccc tgctgcgctg ccgacgccga gccgcccacg catcatcgcg
     2821 caccgcggtg catcgaccga cgagaccgag aacacgatcg ccgcattcaa gcgcgcgtgg
     2881 gacctcggtg tcgaaggtgt cgagctcgat gttcgcgtgt cgcgcgatgg ccaggtcatc
     2941 gtcatgcacg acgacaacac gaagcggctc accggcgtcg acaagctcgt cgccgagcag
     3001 acgctggccg agctgaagaa gctcgagctt ccgaagaagc aacgcatccc gacgctgcgc
     3061 gaggcgatgg cgacgattcc gcccggccgc gcgatgttcg tcgagatcaa gacaggcatc
     3121 gagacggtgc cggccgtcgc cgcggcgatt cgcgcgagct ggccgagcaa cacgacgatc
     3181 atgctgcagg gcttcgacgc cgacaccctc gtcgcgctcg cgaccgccgt cccggatgcg
     3241 cctgcctact ggaccgtcga cccaccgctc gatgaagcga accagccgct gccgtatccg
     3301 cgcgaggtga tcgacgaagc ggtccgccgc aagttcgcgg gcctcgcgct gctcgcgccg
     3361 tccgtcgatg acaacctcct cgcggcggcg cgcgacgccg gcatcctcat cgatgtctgg
     3421 acgctcaacg atgttccgag cctgtcggca tgggcaggtc gcgaagtccg ctggatggag
     3481 acggatcgcc cggatctcgc accgatgcag ccgtgagccc agcgtcaggg cgaagtgcta
     3541 aaagtcaggg atgcgcgcgt acctcgattt gctccgcacc gtgctcgagc acggcgagcg
     3601 ccggacggac agaacgggca cggggacgat ctcgatgttc ggcgcgcaaa cccggtacga
     3661 cctgcgtgag ggcttcccgc tcgtcacgac gaagaaggtc ctgtttccgg cagttgtccg
     3721 tgagctgctc tggttcctcc gcggttcgac caacatcaac gacgatctca agcagcacac
     3781 gcccatctgg gatgcgtggg ccgacgagaa cggtgagctc ggtccgatct acggctacca
     3841 gtggcggcac tggggcgcat cgccacagaa ccccggcggc atcgaccaga tccaggaagc
     3901 gatcgacacg atcaagaggg acccgacgtc gcggcgcatc atcgtgagcg cctggaacgt
     3961 cgcggatatc ccgaagatga agctacctcc gtgtcacgcg ttctttcagt tctacgtgaa
     4021 cgacaagtgg ctcgactgcc agctctacca gcgctctgcc gatctcgccc tcggggtgcc
     4081 gttcaacatc gcgagttacg cgctgctcat gtcgatgatc gcccaggagt gcggcctttc
     4141 gcctcgacac ttcgtccaca ccctcggcga cgcgcatatc tacctgaacc acgtcgaagg
     4201 cgtgaagatt cagctctcac gtgcgccact gccgccaccg acgctcgtgc tcgcgcccgg
     4261 caagaagacc ctcgagtaca cgttcgagga catctctctc gagggttatc aacaccatcc
     4321 gtttatcaag tttccggtcg ccgtctaagt aaacgaggca cgtggcttgc gttgagtagc
     4381 ggttattgaa gcccggaacg atcatagccg gtaggtacga aatcctcgca aagctcggtg
     4441 cgggcggaat gggcgaggtc tttcgtgcac gacaccgttc gctcgacaag gaagtcgcgc
     4501 tgaagctcct cgtcgcagga tcggcggact tcgaggtgag gttcgagcga gaggcgaagg
     4561 ccgccgcgcg cctggatcac cccaactgtg tgcgggtgat cgattacggc aagagcggca
     4621 actaccactt catcgcgatg gacctcctcg tcggtgagac gctagccgcg gcgctatcgc
     4681 gcggtcccat gaccatcgag cgcacggtgc acatcacgaa gggcctcctg tccgccctcg
     4741 accatgcgca ttcgcacggc gtgcttcacc gcgacctcaa accggagaac gtgttcctcg
     4801 tcggtgggcg ccctgtactc atcgacttcg ggcttgccac acttgccggc cacacccgtg
     4861 tcacgggcga gggcatgtgc atgggctcgc cgtcgtacgt tgcgcccgag cgcctgatcg
     4921 gtcagccggc ggatgcgcgt acggatctct acgcgctcgg cgtgatgatg tacgagatgc
     4981 tggccggcac gcgcccattc acgggaccga caccgcacga gacgatgaag cgcgcgctgc
     5041 aaatgccgcc gcgtccgctc gctgcgttgc gccgagacat cccaccgcac ctcgacttcg
     5101 tgatccgcaa agcgctcgtg aaagatccga agcggcggtt cgcgaccgcg atcgacatgc
     5161 tcgaggagct tgccgaagtt ccgttgtacg aattcgagga cgagccggag atgctgctcg
     5221 agcgcgtcga tgaagcgtcg acgaccgcgc tcgtcacgtt ccgcaagccg agcttctttg
     5281 cacggctctg gagctggttc cgctacggcg catggcggtg ggcgagactc gctaccgctc
     5341 gtgcggctcc cacgtccgcg cggctcagcg tggcttagac ctagtgcggc cagatgtcga
     5401 acgtctggac gacgggtgcg gcgggacacg ccggcaactg cacgcgcagt agcgttccgg
     5461 tgtgctcggc cgcggtgccg tcgtcgccga ttccctgcac gaagatcgat ccgtcttcgc
     5521 tcgtcacgcg cagttgatcg aggcgccagc tcatgaactc gacgtcggcg aagcgatcgc
     5581 tgacgaacag cttgccgcag acttcgaacg cgaccttgaa gccggcggga acggtgtcgt
     5641 gaggcccgat gtagcggtcg gtgatgatcg cgacgtcgcg gcctccgacc ttgcgaatga
     5701 tctgcgtctc ggcgagcccc gtctctagac agctcgtgaa gttgtagtcg agtacgctcg
     5761 gggtctcgcc cttttcgatc tgcttttcga gctccgccac ttccgccgcg ccctcccgct
     5821 tcttcttgtc ggtgcacgcg ccggtgcagg tgctgttctc tttgtactcg tcgtagacct
     5881 tcttggtctt gtcgatgagg cgcagcacga gccgcgtgcg attgcattcg gcttgcggcg
     5941 gcagatcgaa gtgattcatc tggagcgata cgccggccag cggtggcgtc ggagaggccg
     6001 catcagcagg gacgaccacc gcagcgtcgc taggcgcggg tacctcgacg agctttttcg
     6061 tgggctctgg cttcggaggc agagcgcctg catcgcatgc agcgagtagt gcgaacggaa
     6121 taagcttggc cattcggatc tagcgtcgag gcgtacgtaa ctattccgct acacacgtcg
     6181 cgcaaggcgt tcgatcttgt atcgaacacc gttttcttcg gcggcctgct cgccgtccca
     6241 gtcggttcgc acaaagccga gctcgtcgag attcgggatg cggacctcgg tctcgaagtg
     6301 accttcgatg cgcgtcacat agacgtagcg aagatcggcg cgcgcgaatg catccttgaa
     6361 gatctcggcg ccgccgacga cgaagatggt ctcgacgttc ggcgcggtgg cggcgtgaat
     6421 cgctgcatcg acagacgcgg cgacgacgac gccttccggc accgtcagct gcttgcgtgt
     6481 gacgacgaca ttgagccggc gcgggagcgg gctgcctttg acctcttcgg actcccacgt
     6541 cttacggccc atgatgatcg cgttgatctt gccttcgctg cacgcgctcg tgacgcggcg
     6601 aaagtgcgcg agatcgcctt tgagcttggg ccagggaagg gcgttggcct tgccgatgcc
     6661 ccaatcgaga tccgctgcga cgacgacatc aaagctcacg ggcgaacgat agtgtaagtt
     6721 cggcgccgtg gcgaccgtgg tgttcacgcc gaatctgaag aggcatgtcg aatgcccgac
     6781 ggaagaggtc gtgggtgcaa ccgttcgtgc cgtgctcgac ggcgtgttcg cgcacaatcc
     6841 aaagctgcgc ggctacgtcc tcgatgatca gggcgcgttg cgtcgtcaca tgatcgtgtt
     6901 cgtcgacggt agtcaaatcg acgatcgcga caacctctcc gatcccgttc gcccgacgag
     6961 cgaggtctac gtcatgcaag cactgtctgg aggctcctga tgaccgcgca atcgatcctg
     7021 gtttcgaccc gcaagggttt gttcgtcgcc gagcctgcgg gcagcagcta caaggtcacg
     7081 cgcggtgcat tcgtcggcga caacgtttcg atctcgatgg tcgatccgcg cgacggcgct
     7141 tggtacgcgg cgctcgatca cggtcacttc ggcgtcaagc tgcatcgctc cgacgacaag
     7201 ggcgcgagct ggaccgagat cacgacgccg gcgtatccgc cgaagcccga aggccaggtc
     7261 gagaagacga tgtggggcaa ggatcgcgcg tggtcgacgc tgctgatctg ggcgatggct
     7321 ccggcgcccg agaaagcagg cgcactgtgg gccggcaccg tgcccggcgg cctgttccgc
     7381 tcggatgatc gcggccagtc atggcgtctc aacgaggcgc tgtggttcca ccccgatcgg
     7441 ccgaagtgga acggcgtcgt gaaagaggaa gcgggcattc actcgatctg cgtcgatccg
     7501 cgtgatccga agatcatctc cgtgggcgtc tcgacgggcg gcgtgtggcg gaccaaagac
     7561 ggcggcgcga catggacgca gtcggcgcac ggcatgcggt ccgagtacgt gcctcccgag
     7621 ctcacgcgtg acggtggcaa tcaagacgtc cactgcctcg tccagtgcgc cgccgatccg
     7681 aagacgttct gggtgcagca ccacaacggc atcttccgtt cgaacgacga catggccacg
     7741 tggaccgagc tctcggagaa cgcgcggccg tcgcagttcg gcttccccgt cgtgtgcgat
     7801 ccaaaggatc ccaacaccgc gtggttcgtg cctgcacaga aggacgagaa gcgcatcccc
     7861 gtcgagggca aggtcgtcgt ctcgcgcacg cgcgatggcg gcaagtcgtt cgaggttctc
     7921 tcgaagggcc tgcccgacac gttcgcgtac gatctgacgt atcgccacgc gctcgcgctc
     7981 tcgaccgacg gcgcactcgc gttcggctcg acgacgggca acgtgttcgc gtctcgagat
     8041 cgcggcgaga gctggtcggt cgtgtcgaac tatctcccgc cagtgcacgc cgttacgttc
     8101 gcggacgtgt aggcagagag accacctcgg cgagaccgac gacgcatgag ataattttcg
     8161 cgtgaagtac ctgctcgtca tcgcgctcct cactgcatgt ggctcccaca tcaagaacgg
     8221 aggcgacgac gacgatgtcg atagtggcct cggcagcaac ggcaacttcc cggattgcga
     8281 tccgtcgaca ttcggcggca tgacgtgcaa cggcaacgcg gttgtctcgt gcaacgcgga
     8341 tggcacgttt ggccagacgg tcacgacgtg cggcaacggc atggagtgtc gcgatggctc
     8401 gtgcaagaac gcgtgcaccg cggacggtgt cgaccttgtc tacgtcgtcg acgagatgaa
     8461 cgacttcatg agcttcgatc cgcggctctt gcccggcgat ccgttccaca agatcggcac
     8521 gctcagctgc ccgcacaaca acacgtcgat ccagaccggc aacaacgtcg cgatgccgtt
     8581 ctcgatgtcc gtcgatcgcg atggcgtggc gtgggtgctc tacacgacgg gcgagctgtt
     8641 caaggtctcg ctccaggacg cgagctgcac gaaggcgaac aacacgatcg gtgcgagcag
     8701 catgttcctg ttcggcatgg gcttcgcgct cgacgcgccg atggtcgaca ccgagaagct
     8761 gttcctcgcc ggcggtaacc gcgatccgag caccacgccg cgccgcctcg gctgggacga
     8821 cacgcacggc aacaacctca cgccgacgaa cgtcggcaac atcgccgccg cgagtgatta
     8881 cagccccgag ctcaccggca ctgccgaagc gaagctctac ggtttctacc cgcgcatcgc
     8941 gcaagtcccg ttcgttcagg agatcgacaa gtccacgggc gccgcggtcg ggcaaacgtg
     9001 gaacgtcgga acaacaggtc tcggcacggg catccgcgat tgggcgttcg cgcagtgggg
     9061 cggcgtgttc tacgtgttca tcacgacgga gacgaacggc gtgcgcaact ccacggtgcg
     9121 cagcgtcgat cgtgcatcga gcgcttacaa agtcgagctg cagaacctgc cgtactacat
     9181 cgacggcgcc ggcgtatcga cctgcgctcc cgcggtcctg cagtagctcc aaaagtgttc
     9241 tgcgcgcgct gatcgcgcca gtgcgcgagg tgtacgttcg gcccgatgcg gaatatcggg
     9301 tggattctcg tcgcggcgtt cgtcatttgc ctcggcgcat gcggcggtaa gaagagcggc
     9361 ccaggaggcc tgaccgtcac gggctcgccg acagggcaaa tcgacggcga ggtgcgcgtc
     9421 attctcgcgt tttccaagcc gatggtcgcg aaggatcgca tcggtcaggt cgcgacgacc
     9481 gcgccgctct cactggtgcc ggatatcgcg cacgagatgc ggtggaccga cgacaagacg
     9541 ctcgtcgcgg tgccgaccgc gagcttgccg atctcgacga agttcacggt gaccgtgccc
     9601 gccgatgcga aggcgctcga tggcagcacg ctcggcaagg caacgagctt cgagttcttc
     9661 accgagcggc tctcgctcac gaccgaggtc gtcggctcga aggaccgcgc ggcgaagaac
     9721 cagctcgtca agctgacgtt caaccacgag gtcgcattcg atcagatcga gaagctgtgc
     9781 tcgttcacgt cgaagagcgg ctcgcagaag gcgaagctcg cgccggattc caatgccgga
     9841 ccggcgaagc agtacctgct cgtgcccgcg gcggacctcg cgctcgacac cgattgggcg
     9901 ctcgcctgca aagcggagct gaagggcgtc gtcggtaacc tcggtctcga aggcccgttc
     9961 gaggagaagt tccacacgta cggcgcgctg aagtacgtcg atcgcgagcc gaaggacaac
    10021 gacatcgttc ccgacgagaa cttgaagatc aagctcgtgt tctcgaaccc gctggccgag
    10081 ccgttcaagc tcaagatcgc gccagcggtc gccggcttcc cgcagggctg tcacgcgatg
    10141 accgacgatc cggtcggcgt cagctgctcg ggcgcgttcg aagcactgac ggagtacacg
    10201 atcaccgtcg acgccgagca gaaagacatc ttcggccaga agctcggcaa ggtcgaacaa
    10261 gtcgacttcc gcacgaccga cgcgaagccg acgatctcga tggaggacgg ctacttcatc
    10321 ggcgagctga agcgtcccgt cgtgccgctg tgggcgcgca acgtgacgaa gctcgacgtc
    10381 acggtcgtcc ccgtcacgca ggacaacttc cacgagatca tcccgctgct cgattggtgg
    10441 gagcacacgc ccgccgactt caagaagacg cacctcaagc ccgtcgcgaa gcagatcgcg
    10501 gtgagcgggc agaagaacaa gtgggtccag agctcgatcg atccggcgac gctgctcggt
    10561 ggccagccgg gaccgggcat gtattacgtc gagatcggct cgccggaagt cgcgaagggc
    10621 gccttcgtcg acggtggccg caagaaggtg ctcgtcaact tcacgaacat cggggtcgtg
    10681 agcaagctgt caccgacgcg cggcgtcgtg tgggcgacga atctcgtgac gggtaagccg
    10741 ctgccgggcg cgagcgtcag cgttcgcgat gccacgggca aagtgacgtg gaacgggacg
    10801 accgacaacg aaggcgtcgc ggtgctgccg ggcaacgaca agctcggcgc gaccgagagc
    10861 gagtacggcg gcgaccacat cgacgacctg cgcatccacg tgtcgcaagg acccgactgg
    10921 acgatgatca atccgtccgc gagcggcggg ctttcgccgt ggtcattcaa cgtctcggtc
    10981 gatcgctcga gcggctcgac gaagctgcgc gggttcatgc acaccgatcg cgggctctat
    11041 cgcccgggcg acaaggtgca cgtgaagggc ctcgcgcgcg tgacgaagct cggcgagccg
    11101 ctcgcggtcc cgaatacggg caagaaggtc gcggtcgagg tcgaaggccc gcaaggcaaa
    11161 tcgatcatga cgacggacgc gaagctgtcg gcgttcggcg gcttctggtt cgatatcgac
    11221 ctgcccggcg atgcgcgcct cggcgactac acgattcgcg cgaagctcga gcacggcacg
    11281 ttcacgcgga cgttctccgt cgaggaattc cgcgcggcga cgttcgaggt gaacggcaag
    11341 acgaaagaga gcctgctcgt ctcgcgcggt gacgtgcacg gcacggtgtc ggcgaactac
    11401 ttctatggcg cgcccgtgcg caacggctcc gtcgacggca atgtctacac gcgcaagcgc
    11461 cgcgttagct tcgatcagct gaaggagttc gacttcggcg acgagcgcag ctgggagtcg
    11521 tactattact acgagtcgaa cgattcgcag tcgatgctca ccgacgcgca cgtgtcgacg
    11581 gacgcaaagg gcaacgcggc gttctcgttc ccggtcagcc ccgacgacgt gtacagcgat
    11641 gccgatctgc tgattcgcgc cgacgtgcac tcgccgtcga acgaagtgat cagcaagtca
    11701 ttcacgatcc cgtacttcag gtcgaagaag tacttcggca tcaagccgcc cgggtacttc
    11761 ctcgacgtga agaagccggc caagttccag gtcgtcagcg cgtcgcccga cggcaagatc
    11821 gtcgacggca gcgcgaaggt gaaggtgacg cgccgcgatt ggaactgcgt ctatgaagac
    11881 tggggctacc gcggctcgta tcagtgcaag gacacgacgc agacgatcct cgagaagacg
    11941 gtcgcgatcg ctggaggcaa gcccgccgag atcgagttca cgcccgcgac cggcggcgat
    12001 tacctcgtcg tcgtcgaagg cgacaaggat gcggccgcgg ccgcgcagcg cctctacgcg
    12061 tggggcgatg acaccggctc gtggcgcagt gacgacacga tgacgttcga ggtcgcgacc
    12121 gacaagcagg agtacaagac cggcgacacc gcgacgctca ttctcaagac ggatctcgcg
    12181 aatgcgacgg gcctcgtgac gatcgagcgc gatggcgtga tcgagaagcg gctcatcgac
    12241 atcacgccga cgacgaagca cctgaagatt ccgatcaccg cggcgatggc gccgaacgtc
    12301 tacgtctcgg ttgccctcgt gcaaggccgc accggcgatg gaccgcgcgg taagccgcgc
    12361 atgcgcatgg gcgtggcgaa cctcaacgtg cgtccggagg acaacaagct cacggtcagc
    12421 gtcgagccgg acaagaagga gtaccggcca ggcgagcagg tgacggcgac cgtgaaggtc
    12481 accgacagca aaggccagcc cgtgtccgcg gaggtctcga tcaccgcggc cgacgaaggc
    12541 gtgctgtcgc tcatcgcgta tcaaacgccg gatccgattc cgacgatgtt cgcgccgtgg
    12601 gggctcggcg tgacgagcgc gacgcagctc gagtacatcc gcgacattcc ggcgccgaac
    12661 gtcgagagac cggccaccgg cggtgactcg gcgggctccg tgcgatcgcg gttcgtcgcg
    12721 agtgcggtgt gggtgccggg cgcggtgacg gatgcaaatg gcgtcgcgac ggtgacgttc
    12781 aaggcgccgg acaacctgac ggcgttccgg ttgatggccg tcgctgccga caagggctat
    12841 cgcttcggct cggccgacaa gcggttcgcg gtgaacaagc cgctgcagct tcactcggcg
    12901 ttgccgcgct tcgttcagct cggtgatgcg gcgaaggcag gcgtcgtcgt tcacaacgag
    12961 accgggaagg cggggcaggc gacggtcaag gtcgtcggcg acgagcacct cgcgatcaaa
    13021 ggtgcgacgg ag
//
LOCUS       DATLBZ010000391        18521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4163974, whole genome shotgun sequence.
ACCESSION   DATLBZ010000391 DATLBZ010000000
VERSION     DATLBZ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18521
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4163974"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VMZ53_19865"
     CDS             <1..302
                     /locus_tag="VMZ53_19865"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HUS30782.1"
                     /translation="LFDLDFDFYVALSALSKNQVMLLLINTVRDGVRGFLPLLANLAG
                     PQETVRKHHRDLIIAVERGDAAGAGRVADEYLRMGAELAARMSGRPELQPQPLPE"
     gene            314..616
                     /gene="gatC"
                     /locus_tag="VMZ53_19870"
     CDS             314..616
                     /gene="gatC"
                     /locus_tag="VMZ53_19870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HUS30783.1"
                     /translation="MPTLTRKEVEEIALLARLYLDSSELEAMQTDLGAILEHFAVLAS
                     VDTTDVVPMTHAVPMDLRLRIDEPLPPLPASEAVRGAPVKDGDLFVVPAIISGSGE"
     gene            613..2073
                     /gene="gatA"
                     /locus_tag="VMZ53_19875"
     CDS             613..2073
                     /gene="gatA"
                     /locus_tag="VMZ53_19875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HUS30784.1"
                     /translation="MTEWLSVAETATKIASGETPAVRVVEKLLERIRSVDRDVKSYLA
                     VDADGARSTAAAIDAARAAGQPLPPLAGVPIAVKDVIVTRGLATTAASRILQGWVPPY
                     DAAVIERLRAAGAIILGKLNCDEFAMGSSTERSAYQKTKNPWDTTRVPGGSSGGSSAA
                     VAAGLCAASLGTDTGGSIRQPAAFCGVVGIKPTYGRVSRWGVIAFASSLDQVGPLARS
                     VHDAAIVLEVISGFDPRDSTSLEVPVPPFSHALTGDIAGLRVGLPKEYFGDGIDAEVR
                     AALERTKAALRERGATLVDISLPHTHLALAAYYIVAPAEASSNLARYDGIRFGKAAEG
                     AKDLLDLYMKSRGAGFGPEVKRRIMLGTYALRSGYYDAYYKKAQQVRTLIKRDFDKAF
                     QYCDVILTPTTPTAAFKRGSKSNPLDMYLGDVFTLSCNLAGLPGLSVPCGLTSEKLPV
                     GAQLLGKPLDEATLLRVGHVIEALCGLRGQIPGGMS"
     gene            2070..3563
                     /gene="gatB"
                     /locus_tag="VMZ53_19880"
     CDS             2070..3563
                     /gene="gatB"
                     /locus_tag="VMZ53_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HUS30785.1"
                     /translation="MSDAHLYETYEAVIGLEVHVQLATHSKAFSSSAVAFGAPANSLT
                     DPVVLGMPGTLPVFNRAALELALKLGIATASKIRAKSRFARKHYFYPDQPKGYQISQY
                     DEPLCEDGHLELILPARRSESGELQSGGGEVKRVRLERIHLEEDAGKNTHVGQFSHVD
                     LNRAGVALVEVVSAPELTSAEEAAEFMRALNRLVRWLEISEADMEKGQMRCDANVSVR
                     LRGEDRLGTRTELKNINSFRFVQNAVEHEILRQMRIVDSGGRVTRETRLWDADSGTSA
                     SMRSKEEANDYRYFPDPDLPPLVVDGSVMASILKTLPELPTARFTRYRAQHGLAADDA
                     RTLISDRALADYFDLVCKVHPGESSGRTIANWMLTELLGALNADGKTIAESPMVPATL
                     SELVRLVETGAISGKIGKDVFAESYKTGEAPGRIVEAKGLQQISDEAALWEIVDRIVG
                     ANQKQADEYAKGKDGLFGFFVGAVMRETKGRANPVITSDLLHKRLGR"
     gene            3635..3952
                     /gene="rplU"
                     /locus_tag="VMZ53_19885"
     CDS             3635..3952
                     /gene="rplU"
                     /locus_tag="VMZ53_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HUS30786.1"
                     /translation="MYAVIQTGGKQYRVEPGKTVVVEKLAGDAGAKVVFDQVLLVSSG
                     DGGSVSVGKPLVAGAKVTGEIVEQTRGEKLVVFKFRRRKNYVRRNGHRQDVTVVKVNE
                     INA"
     gene            3971..4258
                     /gene="rpmA"
                     /locus_tag="VMZ53_19890"
     CDS             3971..4258
                     /gene="rpmA"
                     /locus_tag="VMZ53_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HUS30787.1"
                     /translation="MAHKKGQGSTRNGRDSNPKMRGVKRFAGQVVTAGSILVRQKGTK
                     FHAGENVSIGRDWTLFATINGVVKFERHGKSKMRVAVYPAGDPAVAAPAAA"
     gene            complement(4316..4507)
                     /locus_tag="VMZ53_19895"
     CDS             complement(4316..4507)
                     /locus_tag="VMZ53_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30788.1"
                     /translation="MKALIAALALSTSLAIGTTACAHQQLTNQNVARTALPVAAVVGL
                     VLLGAMYDRNGVDLNGTPR"
     gene            complement(4504..4713)
                     /locus_tag="VMZ53_19900"
     CDS             complement(4504..4713)
                     /locus_tag="VMZ53_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30789.1"
                     /translation="MKAIAAAILAMSLAAGGCAHSQHGSSSSSAPRNVAVTMGVIAAL
                     VLATVIVPCAQCNDTFISPQDGARR"
     gene            complement(4859..5488)
                     /locus_tag="VMZ53_19905"
     CDS             complement(4859..5488)
                     /locus_tag="VMZ53_19905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS30790.1"
                     /translation="MSGEAVLRVERVSIKRGGRTIISELDVEASGGEVVGIVGKNGSG
                     KSTLLMAIAGVLEPTDGRVLIDGASVWGASRDRQRARSQLGYVPENADPPGFLRASEL
                     WALCAASRKTSAPSQALVAALGLDELAELQLERMSLGQRRRACLAAALLGPPRLLVLD
                     EPDNGLDARRIEALVTLLREHAAAGHVALVSTHDRAFLDAIGARLVELA"
     gene            complement(5562..6716)
                     /locus_tag="VMZ53_19910"
     CDS             complement(5562..6716)
                     /locus_tag="VMZ53_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30791.1"
                     /translation="MTWLRVWRRAVIAPAYRRAGAVWVGSAIVGALVFGANGMHPHDL
                     TGLALHRAGVAAVLGTAWLLLFLPTARLLVRPDGAAYLRSLPHAPAAPRALAAAALVG
                     LQLPWLLLWLVGERALGLAIVCAGTLVIAALASWSPRARTYRTPTWSTATVALAAVYI
                     RALTRRASDALVRGIGLAILAGLFGGLMIRNNAATGAHAAVFATASLSLVLVPGWAGA
                     LMPLVEARRQAAWLAHSLGISRSSRLAALYAVVAAIYVATMTIALGATTLIATTSPPP
                     HANAESPVMRLDVTPTPPATTFALLALIAFACGLGAACIATRGILHADVAASDDPRDT
                     PRRGPAAARVVVGAAAGAGLIVLALALLGAIGALGVLVLGIAALATAKDA"
     gene            6767..7771
                     /gene="obgE"
                     /locus_tag="VMZ53_19915"
     CDS             6767..7771
                     /gene="obgE"
                     /locus_tag="VMZ53_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HUS30792.1"
                     /translation="MHFIDEAIIHVKAGDGGNGSAAFRREDGVPRGGPSGGDGGNGGS
                     VIVVADSHLSSLLDYKYKRHYKADRGEDGRNKDQYGAAGVDLTLKVPVGTVIYDEESG
                     DVLVDLDADGATFVLAQGGFGGKGNIHFKTPWNQAPRTAEPGTLGEEKTVRLELKLLA
                     DVGLLGYPNVGKSTFIAKVSRARPKVADYPFTTLVPNLGVVRLTDDRTFVIADIPGLI
                     EGASEGAGLGHQFLRHVERCRVLLHIVEATFTTGPERTPREDFDVINRELARYAPDLA
                     KKPQVVVLNKVDAIDPKDIEEYRKEFAELGIELHAMSAATGDGVQLVLEKVWGLLAES
                     "
     gene            7958..8797
                     /locus_tag="VMZ53_19920"
     CDS             7958..8797
                     /locus_tag="VMZ53_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30793.1"
                     /translation="MAAGSAGAQPKTGTTGTTGAGAKAGQAPAPTPAPAPAPAPAPAG
                     DTAGAPAPAPDLASVKPEDMPAAVRMRRLEQKTQALKERAWQLKARLEMLKEQTLGGG
                     VGAQALIGHADQMGSSFRLIKLVYSLDGTQVFARTDDTAESLYKSKTFDVFAGPISPG
                     NHTLQITASYRGNGYGVFEYLKKFTFNAKGAASFTTGEGKISKVECRGYEKGGAGTPM
                     EKRPAIECKVTDVAPEKPGEQKAPTSPGNTPLPPPAAPAPPAAAPAAGGTAAGSASVS
                     TGK"
     gene            8800..11361
                     /locus_tag="VMZ53_19925"
     CDS             8800..11361
                     /locus_tag="VMZ53_19925"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS30794.1"
                     /translation="MRLPKLASTLAVVCGLGAIHIAGGVAVAAPDAESRIASYESEAQ
                     ALQSNLPQSPNAMSTQSGQKRLVDAQVAYSTGDYDTASIALFDLIGKTNGQEKETATF
                     YLGESLYQKGDYGAARTYFQSIKDTAVGSKYYQQSLVRLVEIAITERDAAAGEDALRS
                     LEQTGAGSPAVAYARGKWAFSQARNADGTYDSTKLDNAIAAFNVVPKGSDYELQATYY
                     LGTTYVAKQDLAKATDTFTNLVTRKPRTNVDRRVIELSQLALGRVYYERDQPAKAIDN
                     YLLVDRRSDLFPTALYEVAWVYVKSKQFDKALTALELLTKLDPQSTKTPTVKILEGNL
                     RIRKAQMIRQAQIAGTVNNEEKADPATEYKKAETIFDETHELYYPSFVSLDRMVKGAL
                     DPASFIDQISGRSTHTFAASAPIPEAAAQWLREEPEVERVVHVEMDLADVEANLTESE
                     AIINRLEGVLASGDKYTLYPALSSRRMRIAAIQHDLIGIRIALHDKAGAVSPDRKSLQ
                     AQYAALGNPEQAYGNRTAATRAEYEKIDEAAREVESTINSSQAIAVALRKYSLDVDLP
                     ADQKVPMQSEIDSATKEARAIEDELAEIHRELVLGKDLAGVGDQDLAAARELRHQLKG
                     ALDNEARSASNPLIGRATVLAQTLESVDAMIDSLETRGLEEIKAVLEAEKKNLAEFKA
                     ELAQDDGEARTIGSQVLAGSFKAVHAKFDDIVTRTDVGGVDVLWSQKEDSDDDYKRLN
                     LARSRDLKQLRDEFRFVLDDASPQPKKEPEPAPAPTEGAPSNSPDKSGTPLDTRVKPG
                     GEKEKTAPTVKPDTKATTPATGTGTQPKTGTTGTQPKTGTTGTTQPKTGTTGGSK"
     gene            11358..14918
                     /locus_tag="VMZ53_19930"
     CDS             11358..14918
                     /locus_tag="VMZ53_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30795.1"
                     /translation="MRTRKPLAILLATLLLGSVAGAQPGTTPPGPSPTPVGNEQPNAP
                     GLGAQQAPQLSAEDLEQLKDVEAEYDQFVGAADDHDKRMRQVARREFDARTAELERRY
                     AERIAKTETDKADRHKRTIEKLEKFVADHPAHQQFTPDAMFRLADLYLDEADEEVDRQ
                     LAAQEAAPPDPNAPDAAAIVADYSKSLGLWERILKEFPSYRQTPSTLYLLAYYGKTKD
                     ERRSLQLFLALTCANRYKWQDPPPPVPGKEEAIKRVEAKTLREVYSDCKPFPDSDPEL
                     INHAWVRGIADYHFTVPGEIDEAIAAYNKVTLGAKDSKLYAEALYKLAWSYYKRDFLE
                     QSIANFDESVKLYDSIVAQGGTPPLELREESVQYIAVAFTDPWNGETDTDPVKAFDRA
                     KNFYKGRENEPHVRDVWVAMGQAFEDLQAWDQAVDAYRIAIGPPWELDPKNPVVHQKI
                     VNVFEQKGDKFAADQAAAELATRYAPGTAWYTANEKDREAMENQRRIAERALYAAARN
                     THAAATTLRKEYEDSAKKDPNTKSEYLALYAKAVDLYRQFVNTYPESDYAYEFNFLMG
                     EALFYAERYPEAIVQYKWVRDHRDLGTTYYLDAARSVLASYEAQRNAQVAAGTLKPLT
                     VPTIAELQAIPQPWSPQPIPESDLQLQQEYDNYQNFVQDPAAAPGQGINAALISLAYL
                     HTDDAIARFKKVMDKFCAAPPAKPGEPAPAAKAKDGILAIYQAQSNFDAIEQTNKAFI
                     TAKCGDATSIQLAINQNRSLNFSRAAELYKQQKYQEAAEAFYRFYKTAQPTDPDLPIA
                     LYNAAVSYKLADKPKTAIALFKEFGARPEKNFRESPYYLDAMRLTAASYQAAFQYDEA
                     IKTYLDLYETTKTAKKKGIKAPDPLPGEKPLTLEQIGLDAVYNAAYAAEVSRDFTRAV
                     KLYTQYRGIEPDRRKQDRALWSVANIFKSSGDVNNMVQQYDAWRAKYGKDPGNEDDYV
                     KTYYETAKLYHAKGRTPQARTAEQGAIDAWKARGAIKNSPGAKMAAEFALNDAEDFYA
                     KRWEPFAVTKPASSTVLKTAKAQVEAQQKQINDLRKNAEDKYIGLDQYGVLEASMAAK
                     VRFGDIQYDRAQKIANIPIPKIIANNDAAVAQFETQRDQNLKKDLDEARLNWEEVANL
                     AKKGGVSNKWSQHALENLAREFPDQYKALRQELVQGTDAP"
     gene            14972..16072
                     /locus_tag="VMZ53_19935"
     CDS             14972..16072
                     /locus_tag="VMZ53_19935"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS30796.1"
                     /translation="MSRRLGALLLCVAIAACGGGSKKSGTTPKAGAGSGAGGKDATSM
                     ADNGDPGGAGGGGGGSATAPGMLQDGTGGDSGGGGSAANEPPVTFPILDPDPAQAKVQ
                     VDAQLTVARTALAKSPPDADGALRAAREALKIDASSVDAAAYVAFAYYHKKQLDTAEL
                     VLDDLFKRPTAKQNANVFYVYGLVYEHTNRPDQAVLAYRKAVEINPSFQSALVNLGAF
                     QLRNKQYDEATQTFEKLTQSGRNDEVTWTSLGSAYRGKSGDYPPGSSDRNGLILKAEQ
                     AYKRATTTNASYGPAYFNLALLYLDADPFPSGGGTLDTLQRLNAAKNWLAQYKNMPGV
                     DIKLFDERNKDIDKAITREQKRRAKAAKTKSG"
     gene            16109..16414
                     /locus_tag="VMZ53_19940"
     CDS             16109..16414
                     /locus_tag="VMZ53_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30797.1"
                     /translation="MRTLAALALVAAAAMPAAAQSRPEGCADDDGSAEKKQGKFHIEY
                     INGKPVTVIDTLVQVCGKVPRPSVVYVLTPKNIDYEWESLKQDFMPLIIATVRKAPF"
     gene            16414..17805
                     /locus_tag="VMZ53_19945"
     CDS             16414..17805
                     /locus_tag="VMZ53_19945"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS30798.1"
                     /translation="MATARAQQQQQQQGPRPRILRIGILLGGKIVEERLIRERTSVTI
                     GQSMKNTFSVPVEGMPLELMLFALDEKGRYHLRFLDKMDGRLSSGDQVNTLDALKGRG
                     AQNAGGYWSVPLSENSRGKLSIGDLTILFQFVTEPPRQPKPMLPASVRGTFADRFDPR
                     LAVIVGASIIIHFAIVIVALTMDVEEESGIADRAYNLTFTQEEYKVDIEQPKPVEVAT
                     NQGGSAAAATPEKKPETKVPTIKKEPEGGGRDKAADVQLQQEENARAMADLLTGEGDN
                     GTSEGDMSKRRPGADLGQQIADVREGGKTVAVGGGAGHGPRGDGDPRVGTGKGPAISG
                     PGGTETAGGPKEEKAPTGRITVNDKHADDDTTLTPDAVLSKIQSAYMRQIKRCYTEYL
                     KKDASARGKVVLSFTVNPTGRAVETGAKGFAGEVDECIGNLMTTWRFPIPKDNDGEDT
                     EASFAITLQLLPD"
     gene            17805..18347
                     /gene="cysC"
                     /locus_tag="VMZ53_19950"
     CDS             17805..18347
                     /gene="cysC"
                     /locus_tag="VMZ53_19950"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HUS30799.1"
                     /translation="MSAGAVVWLTGLSGAGKSTLSAEVGRQLAAADPARRIEVLDGDE
                     IRLYLSQGLGFTRQDRDTNVHRIAYVARLLAKHGVLVLSAVISPYAATRAQIAKLSQD
                     AGHDFVEVFVNAPLATVAERDVKGLYKKAMAGEIANFTGVSDPYEPPDNPAIEVRTDR
                     ESLAESAAKIVDALRVRGLA"
BASE COUNT         3570 a         6153 c         6149 g         2649 t
ORIGIN      
        1 agctgttcga tctcgatttc gacttctatg tcgcgctgtc ggcgctgtcg aagaaccagg
       61 tcatgctcct cttgatcaac acggtgcgcg acggcgtccg cgggttcttg ccgctgctcg
      121 cgaacctcgc cgggccgcag gagacggtgc gcaagcacca tcgcgacctg atcattgccg
      181 tcgagcgcgg cgatgctgcc ggtgccggcc gcgtcgccga cgagtatctg cgcatgggcg
      241 cggagctcgc cgcgcgcatg agcgggcgac cggagctcca gccgcagcca ctgccggagt
      301 aacagagggc gcgatgccga ccctgacgcg gaaggaagtc gaagagatcg cgctgctcgc
      361 gcgcctgtac ctcgacagca gcgagctcga ggcgatgcaa accgacctcg gcgcgatcct
      421 cgagcacttc gccgtgctcg cgtcggtcga caccacggac gtcgtgccga tgacgcacgc
      481 cgtgccaatg gatctgcggc tccggatcga cgagccgttg ccgccgctgc cggcgagcga
      541 ggccgtccgc ggcgcgcccg tcaaggacgg cgatctgttc gtcgtgccgg cgatcatctc
      601 ggggagcggc gagtgaccga gtggttgtcg gtcgccgaga cggccaccaa gatcgcgagc
      661 ggcgagacgc cggccgtccg ggtcgtcgag aagctcctcg agcggatccg gagcgtcgac
      721 cgcgatgtga agtcgtatct ggccgtcgat gccgatggtg cgcgctccac ggccgccgcc
      781 atcgacgcag cacgcgcggc cgggcagccg ctgccaccgc tcgcgggcgt gccgatcgcg
      841 gtgaaggacg tgatcgtgac gcgtggcctc gcgaccaccg cagcctcgcg gatcctccag
      901 ggctgggtgc cgccgtacga cgcggcggtc atcgagcgcc tccgggcagc cggcgcgatc
      961 atcctcggca agctcaactg cgacgagttc gcgatgggca gctcgacgga gcgctcggcc
     1021 taccagaaga ccaagaaccc atgggacacc acgcgcgtcc cgggcggcag ctcgggcggc
     1081 agctcggcgg ccgtcgcggc ggggctgtgc gccgcgagcc tcggcaccga caccggcggc
     1141 tcgatccgcc agccggccgc gttctgcggt gtcgtcggca tcaagcccac ctacgggcgc
     1201 gtgtcgcggt ggggcgtgat cgcgttcgcg tcgtcgctcg atcaggtggg cccgctcgcg
     1261 cgcagcgtgc acgacgccgc gatcgtgctc gaggtgatct cgggcttcga tccgcgcgac
     1321 tcgacctcgc tcgaggtgcc ggtcccgccg ttctcgcacg cgctcaccgg cgacatcgcg
     1381 gggctgcgcg tcgggttgcc gaaggagtac tttggcgacg gcatcgacgc ggaggtccgc
     1441 gcggcgctcg agcggaccaa ggccgcgctg cgcgagcgag gcgcgacgct cgtggacatc
     1501 tcgctgccgc acacgcacct cgcgctcgcg gcgtactaca tcgtcgcgcc ggcggaggcg
     1561 tcgtcgaacc tcgcgcgata cgacggcatc cggttcggca aggcggcgga aggcgcgaag
     1621 gacctgctcg acctctacat gaagtcgcgc ggcgcgggct ttggccccga ggtgaagcgc
     1681 cgcatcatgc tcggcacgta cgcgctgcgc agcggctact acgacgccta ctacaagaag
     1741 gcgcagcagg tccgcacgtt gatcaagcgc gacttcgaca aggcgttcca atactgcgac
     1801 gtgatcctca cgccgacgac gccgaccgct gcattcaagc gcggcagcaa gtcgaaccca
     1861 ctcgacatgt acctgggcga tgtgttcacg ctgtcctgca acctcgccgg actgccgggc
     1921 ctcagcgtgc cgtgtgggct gacgagcgag aaactccccg tcggtgcgca gctgctcggc
     1981 aagccgctcg acgaagcgac gctcctgcgc gtcggtcacg tgatcgaagc gctgtgcggc
     2041 ctccgcggcc agattccggg cgggatgtcg tgagcgacgc gcacctctac gagacgtacg
     2101 aagccgtgat cgggctcgag gtgcacgtcc agctcgcgac gcacagcaag gcattctcgt
     2161 catcggccgt cgcgttcggt gcgccagcca actcgctcac cgatccggtc gtcctcggca
     2221 tgccgggcac gctgccggtc ttcaaccgcg ctgcgctcga gctcgcgctc aagctcggca
     2281 tcgcgaccgc atcgaagatc cgcgcgaagt cgcgattcgc tcgcaagcac tacttctatc
     2341 cggatcagcc gaagggctac cagatctcgc aatacgacga gcctctctgc gaggacggcc
     2401 atctcgagct gattctcccc gcccgccgga gtgaatccgg cgagctccag tccggcggcg
     2461 gtgaggtcaa gcgcgtgcgg ctcgagcgca tccacctcga ggaggacgcc ggcaagaaca
     2521 cgcacgtcgg tcagttctcg cacgtcgatc tcaatcgcgc gggcgtcgcg ctcgtcgagg
     2581 tcgtcagcgc gccggagctg accagcgcgg aagaagccgc ggagttcatg cgcgcgctca
     2641 atcggctcgt gcgctggctc gagatctccg aagcggacat ggagaagggc cagatgcgct
     2701 gcgacgcgaa cgtgtcggtg cgtctacgcg gcgaagatcg cctcggcacg cgcaccgagc
     2761 tgaagaacat caactcgttt cggttcgtcc agaacgccgt cgagcacgag atcctccgcc
     2821 agatgcgcat cgtcgacagt ggcggtcgcg tgacgcgcga gacgcgcctg tgggacgccg
     2881 acagcggcac gtctgcgtcg atgcgcagca aagaagaagc aaacgactac cgctattttc
     2941 ctgatcccga cctgccgccg ctcgtcgtcg acggatcggt gatggcctcg atcctcaaga
     3001 cgctgccgga gctgccgacc gcgcgcttca cgcggtatcg cgcgcagcac ggtctcgctg
     3061 ccgacgacgc acgcacgctg atctccgatc gcgcgctcgc cgactacttc gatctcgtgt
     3121 gcaaggtcca tccgggcgag agctccggcc gaacgatcgc gaactggatg ctcaccgagc
     3181 tgctcggtgc gctcaacgcc gacggcaaga cgatcgccga atcgccgatg gtcccggcga
     3241 cgctgtccga gctcgtgcgg ctcgtcgaga ccggcgcgat cagcggcaag atcggcaagg
     3301 acgtcttcgc cgagagctac aagaccggcg aggcgccggg tcgcatcgtc gaagccaaag
     3361 gactgcagca aatctccgac gaagcagccc tttgggagat cgtcgaccgc atcgtcgggg
     3421 ccaaccaaaa gcaggccgac gagtacgcca aaggcaagga tggcctcttc ggcttcttcg
     3481 tcggggcggt catgcgagaa accaagggtc gggcgaaccc cgtgatcacc tcggatctat
     3541 tgcacaaacg gctaggtcgc taagcaccct tgacgaacgc cgcgcgatca gtaaagtccg
     3601 cgattcgtaa aagcattttc cgcggagcca agtcatgtac gccgtgatcc aaacgggtgg
     3661 aaagcagtat cgagtcgagc caggcaagac cgttgtggtc gagaagctgg ccggcgacgc
     3721 aggcgccaag gtcgtgttcg accaggtgct cctcgtttcg tctggtgacg gcggcagcgt
     3781 gtcggtgggc aagcccctcg tcgctggcgc aaaggtcacc ggtgaaatcg tcgagcaaac
     3841 gcgcggcgag aagctcgtcg tcttcaagtt ccgccgtcgc aagaactacg tccgtcgcaa
     3901 cggtcatcgc caagatgtga ccgtcgtgaa ggtcaacgag atcaacgcgt aggtatcgag
     3961 gtaacgcgtc atggcacata aaaaaggaca aggctctacc cgtaacggcc gcgactccaa
     4021 cccgaagatg cgcggtgtga agcgcttcgc gggccaggtg gtcacggcgg gcagcattct
     4081 ggttcgccag aagggcacga agttccacgc cggcgagaac gtcagcatcg gccgcgactg
     4141 gacactgttc gcgacgatca acggcgtcgt gaagttcgag cgccacggca agtcgaagat
     4201 gcgcgttgcg gtgtacccgg ccggcgatcc cgccgtcgcg gctcctgcag cagcgtagct
     4261 cgcgtcgaag agcagatcgg aagcggcggc cttcgccgct tttcgcgttt tcggatcacc
     4321 ggggcgtgcc gttgagatcg acgccgttgc gatcgtacat cgcgccgagc aggacaagtc
     4381 cgacgaccgc agcgaccggc aatgcggtgc gcgctacatt ctgattcgtc agctgctgat
     4441 gcgcgcacgc ggtcgtcccg atcgcgaggc tggtcgacag cgcgagcgca gcgatgagcg
     4501 ctttcatcgg cgtgctccat cctgcgggct gataaacgtg tcgttgcact gcgcgcacgg
     4561 gacgatgacg gtcgcgagca cgagcgctgc gataaccccc atcgtgacgg cgacgttgcg
     4621 cggtgcgctc gacgacgacg acccgtgttg cgagtgggcg cagccgccgg ctgcgaggga
     4681 catggcgagg atggctgctg cgattgcttt catgcttcga cgatgccgcc acctcgcgtg
     4741 aagctcccag acgggggccg tgaacaacgc gtgaagggcg gctcgcggcc ggctggtaac
     4801 ccacgtgggt acgacccacg caagccggcg taagccgacg caaaaagccg tcgtcgcgct
     4861 acgcgagctc gacgaggcgc gcgccgatcg cgtcgaggaa tgcgcggtcg tgcgtcgata
     4921 caagcgcgac atgcccagcg gccgcatgct cgcggagcaa cgtgacgagt gcttcgatgc
     4981 gacgtgcgtc gaggccattg tcgggttcgt cgaggacgag cagccgcggt ggtccgagca
     5041 gggccgcggc gaggcacgcg cgtcggcgtt ggccgagcga catgcgctcg agctgcagct
     5101 ccgccaactc gtcgaggccg agcgctgcga cgagcgcttg cgagggcgcc gacgtcttgc
     5161 ggctcgcggc gcacaacgcc cagagctcgc ttgcgcgcag aaaacccggc gggtccgcgt
     5221 tctccggcac gtagccgagc tgcgagcgcg cgcgctgccg atcgcgcgac gcgccccaca
     5281 ccgacgcgcc atcgatcagc acacggccgt cggttggctc gagcacgccc gcgatcgcca
     5341 tgagcagcgt cgacttgccc gagccatttt tgccgacgat gccgacgacc tcgccgccgc
     5401 ttgcctcgac gtctagctcc gagatgatcg tgcgcccgcc gcgcttgatg gagacgcgct
     5461 cgacgcgcag cacggcttcg ccgctcatgc gttcctcgct gtgtggtcat cgcgattgct
     5521 ggcgcgccga tcactcgacg cagaggacga gcgacgagac atcatgcgtc cttcgctgtt
     5581 gcgagtgcgg cgatgccgag cacgaggacg ccgagcgcgc cgatagcgcc gaggagcgcg
     5641 agcgcgagga cgatgagccc ggcgcctgcg gccgcaccga cgacaacgcg cgccgcggca
     5701 gggccacgac gaggcgtgtc gcgcgggtcg tcactcgccg cgacgtcggc gtggaggatt
     5761 ccgcgcgtcg cgatgcacgc agcgccgagg ccgcacgcga acgcgataag tgcgaggagc
     5821 gcgaacgtgg tcgcgggcgg cgttggcgtg acgtcgaggc gcatgacggg gctctctgcg
     5881 ttggcgtgtg gcggtggcga tgtcgtcgcg atgagcgtgg tcgcgccgag cgcgatcgtc
     5941 attgtcgcga cgtagatcgc ggcgaccacg gcgtagagcg cggcgagccg cgagctgcgc
     6001 gagatgccga gcgagtgcgc gagccatgcg gcttgccggc gcgcctcgac gagcggcatc
     6061 agcgcgcccg cccagccggg gacgagcacg agcgagagcg aagctgttgc gaacactgcg
     6121 gcgtgagcgc cggtcgccgc gttgttgcga atcatgagac caccgaagag gcccgcgagg
     6181 atcgcgaggc cgatgccgcg gacgagcgcg tcggaggcgc gtcgcgtgag cgcgcggatg
     6241 tagacggcgg cgagggcgac ggtggcggtc gaccacgttg gcgtccgata cgtgcgagcg
     6301 cgcggcgacc acgatgcgag tgcggcgatg accagcgttc ctgcgcacac gatcgccaga
     6361 ccgagcgcgc gctcgccgac gagccagagc agcagccacg gcagttgcag tccgacgagc
     6421 gcggcggccg cgagtgcgcg cggcgcggcg ggtgcgtggg gcagcgatcg caggtacgcg
     6481 gcaccgtcgg gacggacgag caggcgcgcc gtcggcagga acaggagcag ccacgccgtg
     6541 ccgaggacgg cggcgacgcc ggccctgtgc agcgcgagac ccgtgaggtc gtgcgggtgc
     6601 atgccattgg cgccgaacac gagggctccg acgatcgccg agcccaccca gaccgcaccg
     6661 gcgcggcggt aggcgggcgc gatgacggcg cgcctccaga ctcgcagcca cgtcacggcg
     6721 tcgagcctat tcggcacccg catgatttgc tacgtatccc ggcgcgatgc atttcatcga
     6781 cgaggccatc atccacgtga aggcaggaga cggcggcaat ggctccgctg cgttccggcg
     6841 cgaggatggt gtgccgcgcg gcgggccgtc cggcggtgac ggcggcaacg gcggcagtgt
     6901 catcgtcgtc gctgattcgc acctgtcgtc gctgctcgac tacaagtaca agcgccacta
     6961 caaggccgac cgcggcgagg acgggcgcaa caaagatcaa tacggcgcgg ccggcgtcga
     7021 cctgacgttg aaagttcccg tcggcaccgt gatctacgac gaggaatcgg gcgacgtgct
     7081 cgtcgatctc gacgcagacg gtgcgacgtt cgtgctcgcg cagggcggtt tcggcggcaa
     7141 gggcaacatc cacttcaaga cgccgtggaa tcaggcgccg cgaacggcgg agcccggaac
     7201 gctcggcgaa gagaagacgg tgcgcctcga gctcaagctg ctcgcggatg tcgggctgct
     7261 cggttatccc aacgtcggca agtcgacgtt catcgcgaag gtctcgcgcg cgcggcccaa
     7321 ggtcgccgac tatccgttca cgacgctcgt gccgaacctc ggtgtcgtcc gcctgaccga
     7381 cgaccgcacg ttcgtcatcg ccgacatccc ggggctcatc gagggcgcga gcgaaggtgc
     7441 ggggctcggc catcagttct tgcgacacgt ggagcgctgc agggtgttgc tccatatcgt
     7501 cgaggcgacg ttcacgacgg ggcccgagcg aacaccgcgc gaggattttg acgtgatcaa
     7561 tcgcgagctt gctcgatatg ctccggattt ggcgaagaag cctcaggtgg tcgtcctgaa
     7621 caaggtcgac gcgatcgatc cgaaggacat cgaggagtac cggaaagagt tcgccgagct
     7681 cggcatcgag ctgcatgcga tgagcgcggc gaccggcgat ggcgtgcagc tcgtcctcga
     7741 aaaggtctgg ggtctgctcg ccgagtcgtg acccgctcca cacccgaaat ggtgcgacgc
     7801 cggtgagatg agaaaaccgc cggggtcatt ccttgttcgg cccaaatcgc agtgttacaa
     7861 ccgcagcatt ccaaaggact tgcactggtc ggccggtgca tcgagggctg ctatgcgagc
     7921 aatttcgaga gtcgttgttg tccctgcttt gctgctgatg gctgcgggta gcgcgggtgc
     7981 gcaacccaaa acgggaacga cgggaacgac cggggcgggc gcgaaggctg ggcaagctcc
     8041 ggctccgact ccggcacctg cgcctgcacc tgcaccggca ccggctggcg ataccgcagg
     8101 ggctccggct ccagcaccgg acctcgcatc ggtgaagccc gaggacatgc cggcggcggt
     8161 tcgcatgcgc cgtctcgagc agaagacgca agcgctcaag gaacgcgcgt ggcagctgaa
     8221 ggcgcgtctc gagatgctca aagagcaaac gctcggcggc ggcgttggcg cgcaggcgct
     8281 gatcggacac gccgaccaga tgggcagctc gttccgtctg atcaagctcg tttactcgct
     8341 cgacggcacg caggtcttcg cgcgcaccga cgacaccgcg gagtcgctct acaagtcgaa
     8401 gacgttcgac gtgttcgcgg gcccgatctc gccgggcaat cacacgctgc agatcaccgc
     8461 gtcgtatcgc ggtaacggct acggcgtgtt cgagtacctc aagaagttca cgttcaacgc
     8521 caagggcgct gcgagcttca cgacgggcga aggcaagatc tccaaggtcg agtgccgcgg
     8581 ctacgagaag ggtggcgcgg gcacgccgat ggagaagcgc cccgcgatcg agtgcaaggt
     8641 caccgacgtt gcgcccgaga agccgggcga gcagaaggcg ccgacgtctc cgggcaacac
     8701 gccgctgccg ccaccggcag ctcctgcacc tcctgctgca gctccggccg ccggaggaac
     8761 tgccgctggt agcgcctccg tctcgacggg gaagtgaaca tgcgattgcc gaagctcgcc
     8821 tccacgctgg cggtcgtttg tggccttggg gcgatccata tcgcgggtgg agtcgctgtt
     8881 gctgcgccgg acgccgagag tcggatcgcc tcgtacgaga gcgaagcgca ggcgttgcaa
     8941 tcgaacctgc cgcagtcgcc gaacgcgatg agcacgcagt cggggcagaa gcgcctcgtc
     9001 gatgcgcagg tcgcgtactc gaccggtgac tacgacaccg catcgatcgc gctgttcgac
     9061 ttgatcggta agacgaacgg ccaagagaaa gaaacggcga cgttctatct cggcgagtcg
     9121 ctgtaccaga agggcgacta cggcgcggcg cgcacgtact tccagtcgat caaagacacg
     9181 gcggtcggca gcaagtacta ccaacagtcg ctcgttcggc tcgtcgagat cgcgatcacc
     9241 gagagggacg cggccgccgg cgaagacgcg ctgcgctcgc tcgagcagac gggcgcgggc
     9301 agtcccgccg tcgcatacgc gcgtggcaag tgggcgttct cgcaagcgcg caacgccgac
     9361 ggaacgtacg actcgacgaa gctcgataac gcgatcgccg cgttcaacgt cgtgccgaag
     9421 ggctccgact acgagctgca ggcgacctac tacctcggga cgacgtacgt cgcgaagcag
     9481 gatctcgcga aggcgacaga cacgttcacg aacctcgtca cgcgcaagcc gcgcacgaac
     9541 gtcgatcgcc gcgtgatcga gctctcgcag ctcgctctcg gccgcgtcta ctacgagcgc
     9601 gatcagcccg cgaaggcgat cgacaactac ctgctcgtcg accgtcgcag cgacctgttc
     9661 ccgacggcgc tgtacgaagt cgcgtgggtc tacgtgaaga gcaagcagtt cgacaaggcg
     9721 ctcactgcgc tcgagctgct cacgaagctc gaccctcagt cgacgaagac gccgacggtg
     9781 aagatcctcg agggcaacct gcgcatccgc aaagcgcaga tgatccgcca ggcgcagatc
     9841 gccggcaccg tgaacaacga agagaaggcc gacccggcga cggagtacaa gaaggccgag
     9901 acgatcttcg acgagacgca cgagctgtac tacccgtcgt tcgtgtcgct cgatcgcatg
     9961 gtgaagggcg cgctcgatcc agcgtcgttc atcgatcaga tcagcggccg cagcacgcac
    10021 acgttcgcgg cgtcggcgcc gattcccgag gctgctgcgc agtggctgcg cgaagagccc
    10081 gaagtcgagc gcgtcgtgca cgtcgagatg gatctcgcgg acgtcgaagc aaacctcacc
    10141 gagtccgaag cgatcatcaa tcgcctcgaa ggtgtgctcg cgagcggcga caagtacacg
    10201 ctctatccgg cgctgtcgtc tcgccgcatg cgcatcgccg cgatccagca cgacttgatc
    10261 ggcatccgca tcgcgctgca cgacaaggcc ggcgcggtca gccccgatcg caagtcgctg
    10321 caggcgcagt acgcggcgct cggcaatccc gagcaggcgt acggcaaccg caccgcagcc
    10381 acgcgcgccg aatacgagaa gatcgacgag gcagcgcgcg aagtcgaatc gacgatcaac
    10441 tcgtcgcagg caatcgccgt cgcgctccgc aagtactcgc tcgacgtcga cctgcctgcc
    10501 gaccagaagg tcccgatgca atcggagatc gactcggcga ccaaagaagc gcgcgcgatc
    10561 gaagacgagc tcgccgagat tcatcgcgag ctggtgctcg gcaaagatct cgcgggcgtt
    10621 ggcgaccagg atctcgctgc cgcacgtgag ctgcgtcatc agctcaaggg tgcgctcgac
    10681 aacgaggcac gcagcgcatc gaatccgctg atcggacgtg cgacggtgct cgcgcagacg
    10741 ctcgagtcgg tcgacgcgat gatcgattcg ctcgagacgc gcggcctcga agagatcaag
    10801 gcggtcctcg aagccgagaa gaagaacctc gccgagttca aggccgagct cgcgcaagac
    10861 gacggcgaag cccgcacgat cggcagccag gtcctcgccg gcagcttcaa ggccgtgcac
    10921 gcgaagttcg acgacatcgt cacgcgcacc gacgtcggtg gcgtcgacgt gctgtggtcc
    10981 cagaaagagg acagcgacga cgactacaag cgcctgaacc tcgcacgcag ccgcgacctc
    11041 aagcagctgc gcgacgagtt ccgcttcgtg ctcgacgacg catcgccgca gccgaagaag
    11101 gagccagagc ccgcgccggc accgaccgaa ggggcgccaa gcaatagccc cgacaagagc
    11161 ggcacgccgc tcgatacgcg cgtgaagcca ggcggcgaga aggaaaagac cgcgccgacg
    11221 gtgaagcccg acacgaaggc gaccaccccg gcgacgggca ccggcacgca gcccaagacc
    11281 ggcacgaccg gcacgcagcc caagaccggc acgaccggca cgacgcagcc gaagactgga
    11341 acgacgggag gctcgaagtg agaacccgca agcctctcgc tattctgctc gccacactgc
    11401 tcctcggcag tgtcgccggt gcgcagccgg gaaccacgcc gccaggtccg tcgccgacgc
    11461 ccgtcggtaa cgagcagccg aacgcgccgg gcctcggtgc gcagcaagcg ccacagctgt
    11521 ccgccgaaga tctcgagcag ctcaaggacg tcgaggcgga gtacgaccag ttcgtcggcg
    11581 ccgctgatga tcacgacaag cgcatgcgcc aagtcgcgcg tcgcgagttc gatgcacgga
    11641 ccgccgagct cgagcgtcgc tatgccgagc gcatcgcgaa gaccgagacc gacaaggccg
    11701 atcgccacaa gcgcacgatc gagaagctcg agaagttcgt cgccgatcac cccgcgcatc
    11761 agcaattcac gcccgacgcg atgttccggc tcgcggacct gtacctcgac gaggccgacg
    11821 aagaagtcga tcgtcagctc gccgcgcagg aagccgcacc gccggatccg aacgcgcccg
    11881 acgcggctgc gatcgtcgcc gactactcga agtcgctcgg cctgtgggag cgcatcctca
    11941 aggagttccc gagctaccgc cagacgccgt cgacgctgta cctgctcgcc tactacggca
    12001 agacgaagga cgagcgccgc tcgctgcagc tgttcctcgc gctcacctgc gccaatcgct
    12061 acaagtggca ggatccgccg ccgccggtgc ccggcaagga agaggcgatc aagcgcgtcg
    12121 aggccaagac gctgcgcgag gtctactccg actgcaagcc gttcccggat tcggacccgg
    12181 agctcatcaa tcacgcgtgg gtccgcggca tcgccgatta ccacttcacg gtgcccggcg
    12241 agatcgacga agcgatcgcc gcgtacaaca aggtcaccct cggcgcgaag gacagcaagc
    12301 tctacgccga agcgctctac aagctcgcgt ggagctacta caagcgcgac ttcctcgagc
    12361 aatcgatcgc gaacttcgac gagagcgtga agctctacga ctcgatcgtc gcgcaaggcg
    12421 gcacgccacc cctcgagctg cgcgaagaat ccgtgcagta catcgcggtc gcgttcacgg
    12481 atccgtggaa cggcgagacc gacaccgatc cggtgaaggc gttcgaccgc gcgaagaact
    12541 tctacaaggg tcgcgagaac gagccgcacg tgcgcgacgt ctgggtcgcg atgggccagg
    12601 cgttcgaaga tctgcaagcg tgggatcagg ccgtcgacgc gtatcgcatc gcgatcggac
    12661 cgccgtggga gctcgacccg aagaacccgg tcgtgcacca gaagatcgtc aacgtgttcg
    12721 agcagaaggg cgacaagttc gcggctgatc aagcggccgc cgagctcgcg actcgctatg
    12781 cgccgggcac ggcttggtac acggcgaacg agaaagaccg cgaggcgatg gagaaccagc
    12841 gccggatcgc ggagcgcgcg ctgtacgcag cagctcgcaa cacgcacgct gccgcaacga
    12901 cgctgcgcaa ggagtacgag gatagcgcga agaaagatcc gaacacgaag agcgagtacc
    12961 tcgcgctgta tgcgaaggcg gtcgatctct atcgccagtt cgtgaacacg tatccggaga
    13021 gcgactacgc ctacgagttc aacttcctga tgggcgaggc gctgttctac gcggagcgct
    13081 acccggaagc gatcgtccag tacaagtggg ttcgcgatca ccgcgatctc ggcacgacgt
    13141 actacctcga cgcagcgcgc tcggtgctcg cgtcgtacga agcgcagcgc aacgcgcaag
    13201 tcgcggcggg tacgctgaag ccgctcacgg ttccgacgat tgcggagctg caagcgatcc
    13261 cgcagccgtg gtcgccgcag ccgattccag agagcgatct ccagctccag caggaatacg
    13321 acaactatca gaacttcgtg caggaccccg cggctgcacc ggggcagggc atcaacgcgg
    13381 cgctcatcag cctcgcgtat ctccacaccg acgacgcgat cgctcgcttc aagaaggtga
    13441 tggacaagtt ctgcgccgcg ccgcctgcga agccgggtga gccagcgcct gccgcgaaag
    13501 cgaaagacgg cattctcgcc atctaccagg cgcagtcgaa cttcgacgcg atcgagcaga
    13561 cgaacaaggc gttcatcact gcgaagtgcg gcgatgcaac gtcgatccag ctcgcgatca
    13621 accagaaccg ctcgctgaac ttctcccgcg ccgccgagct ctacaagcag cagaagtacc
    13681 aggaggccgc cgaggcgttc tatcgcttct acaagacggc gcagccgacg gatccggatc
    13741 tgccgatcgc gctctacaac gcggccgtca gctacaagct cgccgacaag ccgaagacgg
    13801 cgatcgcgct gttcaaagag ttcggcgcgc gtcccgagaa gaacttccgc gagagcccgt
    13861 actacctcga cgcgatgcgc ctcacggctg cgagctatca ggccgcgttc cagtacgacg
    13921 aggcgatcaa gacgtatctc gatctctacg agacgacgaa gaccgcgaag aagaagggca
    13981 tcaaggctcc tgacccgctg ccgggcgaaa agccgctgac gctcgagcag atcggtctcg
    14041 acgctgtcta caacgccgcg tacgccgccg aggtgtctcg cgacttcacc cgcgcggtga
    14101 agctgtacac gcaataccgc ggcatcgaac cggatcgccg caagcaggat cgcgcgctgt
    14161 ggtcggtcgc gaacatcttc aagtcgagcg gcgacgtgaa caacatggtc cagcaatacg
    14221 acgcgtggcg cgcgaagtac ggcaaggatc cgggcaacga agacgactac gtcaagacgt
    14281 actacgagac cgcgaagctc tatcacgcga agggacgcac cccgcaggcg aggacggccg
    14341 agcagggagc gatcgatgcg tggaaggcgc gcggcgcgat caagaactcg cccggtgcga
    14401 agatggcggc cgagttcgcg ctcaacgacg cggaagattt ctacgcgaag cgctgggaac
    14461 cgttcgcggt cacgaagccg gcgtcgagca ccgtgctcaa gacggcgaag gcgcaggtcg
    14521 aggcgcagca gaagcagatc aacgacctcc gcaagaacgc cgaggacaag tacatcggtc
    14581 tcgatcagta cggcgtgctc gaagcgtcga tggccgcgaa ggtccgcttc ggtgacatcc
    14641 agtacgaccg cgcgcagaag atcgcgaaca ttccgatccc gaagatcatc gcgaacaacg
    14701 acgcggccgt cgctcagttc gagacgcagc gcgatcagaa tctgaagaag gatctcgacg
    14761 aagcaaggct caactgggaa gaggtcgcga acctcgccaa gaagggtggc gtctccaaca
    14821 agtggagcca gcacgccctc gagaacctcg cgcgcgagtt ccccgaccaa tacaaggcgc
    14881 tgcgccaaga gctggtgcag ggaacggatg cgccatgatg tcgacgactt acacgccaga
    14941 tcgcgacggc gtttcgcgcg tttataccca cgtgagccga cgcctcggag cgctccttct
    15001 atgtgttgcg atcgccgcgt gcggcggtgg cagcaagaag agcggaacca cgccgaaggc
    15061 cggcgcgggc agcggagccg gcggcaaaga cgcgacgtcg atggccgaca acggcgatcc
    15121 aggtggcgcc ggcggcggtg gcggtggatc ggcgacggcg cctggcatgc tgcaagacgg
    15181 aaccggtggt gactccggtg gcggaggctc tgctgcgaac gagccgcccg tcacgttccc
    15241 gattctggat ccggatcccg cgcaggcgaa ggtccaagtc gatgcacagc tgacggtggc
    15301 tcgtaccgcg ctcgcgaagt cgccgcccga tgccgacggt gcactgcgcg ctgcgcgcga
    15361 ggcgttgaag atcgacgcgt ccagtgtcga tgcagcggcg tacgtcgcgt ttgcctacta
    15421 tcacaagaag cagctcgaca cggcggagct cgtcctcgac gatctgttca agcgcccgac
    15481 ggcgaagcag aacgcgaacg tgttctacgt ctacggtctc gtctacgagc acacgaatcg
    15541 cccggaccaa gcggtcctcg cgtatcgcaa ggccgtcgag atcaacccga gcttccagag
    15601 cgcgctcgtc aacctcggcg cgttccagct gcgcaacaag cagtacgacg aagcgacgca
    15661 gacgttcgag aagctgacgc agagcggccg caacgacgaa gtcacgtgga cctcgctcgg
    15721 cagcgcgtat cgcggcaagt cgggcgacta cccgccgggc tcgtcggacc gcaacggtct
    15781 cattctcaaa gcggagcagg cgtacaagcg cgcgacgacg acgaacgcga gctacggccc
    15841 cgcgtacttc aacctcgcgc tgctctacct cgacgcggat ccgttcccgt ccggcggcgg
    15901 cacgctcgac acgctgcagc gcctcaacgc ggcgaagaat tggctcgctc agtacaagaa
    15961 catgccaggc gtcgacatca agctgttcga cgagcgcaac aaggacatcg acaaggcgat
    16021 cacgcgcgag caaaaacgcc gcgccaaagc cgccaagacg aagagtgggt agccgatcac
    16081 ctcgaggtgc ctcatcatga acacagccat gcgcactctt gctgctctcg ccctcgtcgc
    16141 tgctgccgca atgccggccg ccgcgcagag ccggcccgag ggctgcgccg acgatgacgg
    16201 ctccgccgag aagaagcagg gcaagttcca catcgagtac atcaacggca agccggtcac
    16261 cgtgatcgac acgctcgtgc aggtgtgcgg caaggtccct cgcccgagcg tcgtctacgt
    16321 gctcacgccg aagaacatcg actatgaatg ggagagcctg aagcaggact tcatgccgct
    16381 catcatcgcc accgtcagaa aggcgccgtt ctgatggcca ccgcacgcgc acaacagcag
    16441 cagcaacagc agggaccgcg cccgcgcatc cttcgcatcg gcattctgct cggcggcaag
    16501 atcgtcgagg agcgtctcat tcgtgagcgc acctccgtca cgatcggcca gtcgatgaag
    16561 aacacgttct cggttccggt cgagggcatg ccgctcgagc tgatgctgtt cgcgctcgac
    16621 gagaagggcc gctaccacct tcgcttcctc gacaagatgg atggccgtct gtcgtcgggc
    16681 gaccaggtaa acacgctcga tgcgctcaag ggccgcggcg cgcagaacgc cggcggttac
    16741 tggagcgttc cgctatcgga gaactctcgc ggcaagctct cgatcggcga cctgacgatc
    16801 ctgtttcagt tcgtcaccga gccgccgcgc cagcccaagc cgatgctgcc cgcgtccgtg
    16861 cgcggcacgt ttgccgatcg cttcgatccg cgcctcgcgg tcatcgtcgg cgcatcgatc
    16921 atcatccact tcgcgatcgt gatcgtcgcg ctcaccatgg acgtcgaaga agagagcggc
    16981 atcgccgacc gcgcgtacaa cctgacgttc acgcaggaag agtacaaggt cgacatcgag
    17041 cagccaaagc ccgtcgaagt tgcgacgaac caaggcggca gtgcagcggc ggcgacgccc
    17101 gagaagaagc cagagacgaa ggttccgacg atcaagaagg agcccgaagg cggtggtcgc
    17161 gacaaggccg ccgacgtcca gctccagcaa gaagagaacg cgcgcgcgat ggccgacctc
    17221 ctcaccggcg aaggtgacaa cggcacgagc gaaggcgaca tgagcaagcg ccgcccaggc
    17281 gccgatctcg gccagcagat cgccgacgtc cgcgaaggtg gcaagaccgt cgccgtcggt
    17341 ggcggcgcag gtcacggccc gcgcggcgat ggcgatccgc gcgttggtac gggcaaaggc
    17401 cccgccatca gcggacccgg cggcaccgag acggcaggcg gcccgaagga agagaaggca
    17461 ccgaccggtc gcatcaccgt caacgataag cacgccgatg acgacacgac gctcacgccc
    17521 gacgccgtcc tctcgaagat ccagagcgcg tacatgcgcc agatcaagcg ctgctacacc
    17581 gagtacctaa agaaggacgc gagcgcccgc ggcaaagtcg tcctgtcgtt caccgtcaac
    17641 ccgacgggcc gcgcagtcga gacaggtgcg aagggcttcg ccggcgaagt cgacgagtgc
    17701 atcggcaacc tgatgacgac ctggcgcttc ccgatcccga aggacaacga cggcgaagac
    17761 accgaagcca gcttcgcgat cacgctgcag ctgctgcccg actgatgtcc gccggcgccg
    17821 tcgtctggct caccgggctc tcgggcgccg gcaagtcgac gctgtcggct gaagtcggtc
    17881 gtcagctcgc cgccgccgat cccgcgcgcc gcatcgaggt cctcgacggc gacgagatcc
    17941 gcctctacct ctcccaaggc ctcggcttca cccgccaaga ccgcgacacg aatgttcacc
    18001 gcatcgcgta cgtcgcgcgc ctgctcgcca agcacggcgt gctggtcctc tccgccgtca
    18061 tctcgccgta cgccgccaca cgcgcgcaaa tcgccaagct ctcgcaggac gctggccacg
    18121 acttcgtcga agtcttcgtc aacgcgccgc ttgcgacggt cgccgagcgc gacgtcaaag
    18181 gcctctacaa gaaggccatg gccggcgaga tcgcaaactt cacgggcgtc tccgacccgt
    18241 acgagccacc ggacaatcca gcgatcgaag tccgcacgga ccgcgagtcg ctcgccgagt
    18301 cggctgcgaa gatcgtcgac gcccttcgcg tccgcggcct ggcttagccc ggcagtgcgc
    18361 gaagcagccg cgcgggattt cccgcgtaga cgccgggctt cgtgatcgac ttcgtgacga
    18421 ccgcccccgc cccgatgacg acgtcgtcgc agatctcgac gggaagaatc gtcgcgttcg
    18481 agccgatgct cacgcgattg ccgaccttcg tcgatttcca g
//
LOCUS       DATLBZ010000392         3053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2696692, whole genome shotgun sequence.
ACCESSION   DATLBZ010000392 DATLBZ010000000
VERSION     DATLBZ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696692"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VMZ53_19955"
     CDS             <1..184
                     /locus_tag="VMZ53_19955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30800.1"
                     /translation="QSEAVRRSIPCVLQHGFEVVGDHTIERGRLRPAGVISARQRRCG
                     GARAALERDARATPRM"
     gene            complement(455..1603)
                     /locus_tag="VMZ53_19960"
     CDS             complement(455..1603)
                     /locus_tag="VMZ53_19960"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUS30801.1"
                     /translation="MWRLAVLGLVGCSFDGPRPASSCGAGEELCGETCVAIATDVANC
                     GACGAVCDGRCEEGACISACALSLSSRVQDAWGAWWDGIDRPPMPLSTAAAACSGFGA
                     RLPQASEMFRVRKTGQNPLSNNVAMPKLWSNAPEDPTLQVTVSLLDGGIFGADITMPQ
                     PYRCTCPAPLPEGSPLACHGPLQAGSHVSAGSAAYDRSPDAVKTLVGEGATGVDSLAG
                     FVKAMTAPRHVWVMVPAGGPTESTVTELGNLLELGDTVIDGGNSFFKDDIRRAKAVAG
                     KKIGYVDPARAAASTASNAACTIPSSSGCSSSSTAPRSGSRDRADSSQPRRSGRGDGG
                     ASGSSRTPTTRNPTTIASRSTRGGRRAPNLVALEPQTRRIAARPSSEL"
     gene            complement(1875..2405)
                     /locus_tag="VMZ53_19965"
     CDS             complement(1875..2405)
                     /locus_tag="VMZ53_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30802.1"
                     /translation="MPTVPLDHEQAAEVIDAMRLRAAQLRTLQLADDCKYRFGSGTMT
                     AEAARAFGALVGELRAIEKLDLAATSLDDRGAYALARLQAETLVDLDISRTKITLDGL
                     RAILANPRLHHLRRLDLGGITLGEEGAALLAATPFTQLETLNLMNCGVDAEPFRAKYK
                     TVVGDEIWAPVEGYDD"
     gene            2542..>3053
                     /locus_tag="VMZ53_19970"
     CDS             2542..>3053
                     /locus_tag="VMZ53_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30803.1"
                     /translation="MLDHYRTQPSARMVHFNFMARGEPLLNHTVLHASSALFDALWQR
                     ADAFDLRARFLVSTILFVEHGDRPLEDVFRRFHPEIYYSLYSMSPAFRRRWLPKAQPA
                     EVALDRLAAWQRATYKIVKIHYAFIAGENDSERDVHAVCDALESRDLYTHVNFVRYNP
                     PSPAHGEEPP"
BASE COUNT          493 a         1042 c         1019 g          499 t
ORIGIN      
        1 gcaatccgaa gccgtgcggc gatcgatccc gtgcgtcctg cagcatggtt tcgaggtggt
       61 cggcgatcac acgatagaac gcggtcgcct gcggccggcg ggggtgatat ccgcgcggca
      121 gcggcgctgc ggcggcgccc gcgcggcgct cgaacgtgat gcgcgggcga caccacgcat
      181 gtagtgcgcg cgcgatacca tcgtcgtaac ggaactacag cgtgatggat cgcgtcgtta
      241 cgcttctatg tccgaccacc ggacacgcat gtccgcatac cggacgccat ggccatggca
      301 gtggccagat gccttccgct cgtcggtcac gccgacgatg gctcggctcg ctcggcgttg
      361 catcgcgatc gcgacctcgt gcatcccgtc agccggcagc tcaggttcga gcgtcgggag
      421 gttcgcgagc gcgatcagcg cggtgtggca gagctcagag ctcggaggac ggccgcgctg
      481 caatccgtcg tgtttgtggc tcgagcgcga ccaggttcgg cgcgcgtctc ccaccacgcg
      541 tcgagcgtga agcaatggtc gtagggtttc gtgtggtggg cgttcgcgac gaaccgctcg
      601 caccgccgtc gccgcggcca gatcgccgcg gctgcgatga atccgcacga tctcgcgatc
      661 ccgaccgtgg cgctgtgctc gacgatgaac agccgctgga ggacgggatc gtgcaagccg
      721 cgttcgaggc cgtagatgcc gccgctcgtg ccgggtcgac gtagccgatc ttcttgccgg
      781 cgaccgcctt cgcgcggcgg atgtcgtcct tgaagaacga gttgccgcca tcgatgaccg
      841 tgtcgcccaa ttcgagcagg ttgccgagct cggtgacggt cgactccgtc gggccacccg
      901 cgggcaccat cacccagacg tggcgcggcg cggtcatcgc cttgacgaag ccggcgagcg
      961 agtcgacgcc ggtcgcgccc tcgccgacga gcgtcttcac ggcgtcgggg ctgcggtcgt
     1021 aggccgcgga tccagcggag acatgacttc cggcttgcag cggaccgtgg cacgcgagcg
     1081 gcgagccttc cggcagaggg gcagggcacg tgcagcggta gggctgcggc attgtgatgt
     1141 ctgccccgaa gatgccgcca tcgagcaacg agaccgtgac ctggagcgtt gggtcttcgg
     1201 gcgcgttcga ccacagcttg ggcatggcga cgttgttgga gagcggattc tggccggtct
     1261 tgcgcacgcg aaacatctcg ctcgcttgcg gcaaccgcgc cccgaaaccc gagcacgctg
     1321 cggctgccgt cgagagcggc atcggcggcc ggtcgatgcc gtcccaccac gcgccccacg
     1381 cgtcttgcac gcgcgacgac agcgagagcg cgcacgcgct gatacacgcg ccttcctcgc
     1441 aacggccgtc gcacaccgcg ccgcacgcac cgcagttggc gacatcggtc gcgatcgcga
     1501 cgcatgtctc gccacagagc tcctcgcccg caccgcacga gctcgcgggg cgtggcccgt
     1561 cgaacgagca gccgacgagg ccgagaaccg cgagccgcca cacgcgcgca gcgtaccaaa
     1621 ctaaccgctg cgaggatcga tcgacgctcg aggcgctcag cgccgtcgct tcttgcgaga
     1681 cgtcacgact ggcaccgcga taccgagcgt cgcgagatcg atacgaccgg ccgcttgatc
     1741 gccgcgacgc gggtgacgat gcgcggatac tcgcccgcgt cgcttcgatc agtccggcgc
     1801 cctcgaggga acgcagcatg tcgagcaccg aatcggctca tacagcaagc gctcgagctc
     1861 gagcgtgcgg atgctcagtc gtcgtagcct tcgaccgggg cccaaatctc gtctccgacg
     1921 acggtcttgt acttcgctcg aaacggctct gcatcgacgc cacagttcat gaggttcagc
     1981 gtctcgagct gcgtgaatgg cgtcgccgcg aggagggccg cgccttcctc gccgagcgtg
     2041 atgcccccga gatcgagcct tcgcaagtgg tgaagccgcg ggttggcgag gatcgcgcgc
     2101 aggccatcga gcgtgatctt ggtgcgcgag atgtcgaggt cgacgagggt ctcggcttgc
     2161 aatcgagcga gggcgtacgc gccgcgatcg tcgagcgacg tggcggcgag gtcgagcttc
     2221 tcgatcgcgc ggagctcgcc gacgagcgcg ccgaacgctc gtgccgcctc ggcggtcatc
     2281 gttccactgc caaagcggta cttgcaatcg tcggcgagtt gcaacgtgcg caactgcgcc
     2341 gcacggaggc gcatcgcatc gatcacctcg gctgcttgtt cgtggtcgag aggaaccgtc
     2401 ggcaccgcgg actacgtcgt cgtgtacctg tcctcgcaga ccgggtgccg gcaagcctgc
     2461 cgcatgtgtc acttgacggc cacggggcag acgcggcttc gcgacacgac ccacgacgag
     2521 ctcgtcgaac aagccgatcg cgtgctcgac cactatcgga cgcagccgtc cgcgcgcatg
     2581 gtgcacttca acttcatggc acgcggcgaa ccgctgctca accacacggt gcttcacgct
     2641 tccagcgcgc tgttcgatgc actgtggcag cgtgctgacg cgttcgacct ccgcgcacgg
     2701 ttcctcgtat cgacgatcct tttcgtggag cacggcgacc gccccctcga ggacgtgttt
     2761 cggcgcttcc acccggagat ctactattcc ctctattcga tgtccccggc ctttcgccgg
     2821 cgctggttac cgaaggcgca gcccgcggag gtcgcgctcg atcggctcgc ggcctggcaa
     2881 cgggcgacct acaagatcgt caagatccac tacgcgttca tcgccggaga gaacgacagc
     2941 gagcgtgatg tgcacgccgt atgcgacgcg ctcgagtcgc gcgacctcta cacgcacgtc
     3001 aacttcgtcc gctacaaccc tccaagcccc gcgcacggtg aagagccgcc cga
//
LOCUS       DATLBZ010000393         5562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2697477, whole genome shotgun sequence.
ACCESSION   DATLBZ010000393 DATLBZ010000000
VERSION     DATLBZ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2697477"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VMZ53_19975"
     CDS             <1..411
                     /locus_tag="VMZ53_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30804.1"
                     /translation="DLDIDSADRVAGDYHHYDMGLVFDFRRDPSVEQHYFILETEQGK
                     HLMTVATAGGWRFLNVLDGSFTSAGPIDGEQKTSGDPAALSIVFEVEEMKLLEPLRLA
                     LTAHGIDDDLQQMPLSNIDTTQQELWMPGMRPMM"
     gene            complement(418..1230)
                     /locus_tag="VMZ53_19980"
     CDS             complement(418..1230)
                     /locus_tag="VMZ53_19980"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS30805.1"
                     /translation="MQLQDRVVVITGAGSGIGRATALAFAKQGARIAACDIDQQRLDT
                     LAADLGDRHLLSRKLDVSDRLAMRLFADEVHAHAPAADVVVNNAGVGLGGPFLDTSLD
                     DWDWILGINLKGVVHGCHFFIPKMVERGAGGHVVNIASILGIYAAGNVTAYAATKFAV
                     RGLSLSLRDELAPHKIGVTAICPGMINTAIIDDGRMAGTIGERRTRVSKTFKDRGAAP
                     EKVAAAILDAVHTNPAVRPVGADAVVISALQRLAPRALARLGGQLQRRFGAV"
     gene            complement(1234..2046)
                     /locus_tag="VMZ53_19985"
     CDS             complement(1234..2046)
                     /locus_tag="VMZ53_19985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HUS30806.1"
                     /translation="MSIPIPRALDVDLTKVPRHWLAGNAAATAISNGINLLFPHGERF
                     FVRSVNAYFDQIKDPTLRSHVKAFFKQEGHHAKAHDDFNAVLRAQGFEIDTFLGRYRA
                     VSTWLEKRVPAKLNLAGTAAAEHFTAILAEGAFKRGILDSLHPLMQQLLAWHAAEEIE
                     HKAVAFDVLTEVDDSYALRMAGLAYATIMLASFWAWGTAMLLRQEKLGLRGTLRELRK
                     MRKDDPIIQRVFIKGIRQYMRRAFHPNDNANEGLAAEWFAARGLPFPVPEAA"
     gene            2111..2779
                     /locus_tag="VMZ53_19990"
     CDS             2111..2779
                     /locus_tag="VMZ53_19990"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS30807.1"
                     /translation="MPPRRPIRRLIRAAVAPRKRRIRLDNDERRAQLLTLARKAFSDR
                     SYDDVSIDDLAREAKISTGLLYHYFPTKRDLYVAGLHEIAEELVERCTAIPANLPPID
                     RVRTGLDAYLDHISQHARAYVSLMRGGIGSDPDVAAIVEGVRKRLTDNFLEQTPFAAM
                     LVGDVRFQTAVRGWIGFVEGATIDWCANQRMPREDLRELLTQILFAIMSTVAPQVIKA
                     ATVS"
     gene            2779..3696
                     /locus_tag="VMZ53_19995"
     CDS             2779..3696
                     /locus_tag="VMZ53_19995"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HUS30808.1"
                     /translation="MSRSVVIIDGFAPEARALRATFDERFAEPRSTRGDRFVWDWWHV
                     PGQYTALRTPAWTYFPKKVYEAFHRRLVEWGRKTLGCHDISPPWLSMYVDGCRQELHG
                     DLPHGPWAFVYSLTNWKGRVFRGGETLLVRDEVLDFWHDFRSVRAVEEPELIRAVEPR
                     FDRLTVFDPRIPHGVREVTGTRDPREGRLVIHGWFVQPRPFIRGPLPTKLLTQRAKDL
                     GGEIGTWLDELGDISIAGLLAVAFGVDRRGKVGRVHVLSDTTRVPKLEEKIRQQLLRR
                     IQASISGWTFGAQRAPSAVTLPLVFEQGG"
     gene            complement(3693..4220)
                     /locus_tag="VMZ53_20000"
     CDS             complement(3693..4220)
                     /locus_tag="VMZ53_20000"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS30809.1"
                     /translation="MSRFGIREAKLGDAEAIARAHTASWQSSYRGILPDTVLDRIDVG
                     QRAASRRQIISDRSIYQLVAYDLTHGDIVGLCDAGPSRRPLPSLPTHATGEVYAIYLV
                     QHAKRHGLGQEMFEQVMAHFARNGIRQMVVWVLDNNHHARRFYETLGGRESSRLQTRV
                     GGFPVTELAYVWDRF"
     gene            complement(4217..5491)
                     /locus_tag="VMZ53_20005"
     CDS             complement(4217..5491)
                     /locus_tag="VMZ53_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS30810.1"
                     /translation="MKVFVIDDEREMVDLIQLGLKKRGFSVVPFNSGADALAALTEHD
                     VDVVVTDLNMKGMNGLDVCQRIETDRPDIPVIVLTAFGSFETAVGAIRAGAYDFVTKP
                     VEIEALAIAIRRAAEHRQLRGEVKRLREVVASTRGRGDLIGASPAMQQVYDLIDQVSA
                     TDATVLITGESGTGKEVVARAVHEKSRRKAGPFIAVNCAAVPEATLDTELLGQFQQAT
                     NGTLFLDEIGEMAPALQSKLLRAIQEHKVRLVAATSHDLEEMVEDKRFREDLYYRINV
                     IHIPLPPLRARGGDVLLLAQHVLRKYAAVFGKKVIGISPAAAERLVAYDWPGNVRELS
                     NSIERAVALAHFEEIQVEDLPEKVRDRQNVRSPAHSGGEVPELLTLEEVERRHVLRVL
                     EACNSNRTDAAKILGLDRKTLYRKLLRWGVSE"
BASE COUNT          882 a         1630 c         2005 g         1045 t
ORIGIN      
        1 gacctcgaca tcgatagcgc ggatcgcgtt gcgggcgatt accaccacta cgacatgggt
       61 ctcgtcttcg acttccggcg cgacccgagc gtggagcagc actacttcat tctcgagacc
      121 gagcagggca agcacctgat gaccgtggcg accgctggcg gctggcgctt cttgaacgtg
      181 ctggatggca gcttcacgag cgcggggccg atcgacggcg agcagaagac gagcggcgat
      241 cctgcggcgc tgtcgattgt cttcgaggtc gaggagatga agctgctcga gccgctgcgt
      301 ttggcgctca ccgcgcacgg catcgacgac gacctgcagc agatgccgct gtcgaacatc
      361 gatacgacgc agcaggagct gtggatgcct gggatgcggc cgatgatgtg aggagagtta
      421 gacggcgccg aagcggcgct gtagctggcc gccgagtcgc gcgagagcgc gtggggctag
      481 gcgctggagt gcggagatga cgaccgcgtc ggcgcctacg gggcgaacgg cggggttcgt
      541 gtggacggca tcgaggatcg cggccgcaac cttttcgggg gcggcaccgc ggtccttgaa
      601 cgttttggag acgcgcgtgc ggcgctcgcc gatcgtgcct gccatgcggc cgtcgtcgat
      661 gatggcggtg ttgatcatgc ctgggcagat ggcggtgacg ccgatcttgt gaggcgcgag
      721 ctcgtcgcgg agcgagaggg agaggccgcg gacggcgaac ttcgtggcgg cgtaggcggt
      781 gacattgcct gcggcgtaga tgccgaggat ggaggcgatg ttgacgacgt ggccgccggc
      841 gccgcgctcg accatcttcg ggatgaagaa gtggcagccg tggacgacgc ccttgagatt
      901 gatgccgagg atccagtccc agtcatcgag cgacgtgtcg aggaacgggc cgccgaggcc
      961 gacgcctgcg ttgttgacga cgacgtcggc ggcgggcgca tgcgcgtgga cttcgtcggc
     1021 gaataggcgc atcgcgaggc ggtcggagac gtcgagcttg cgcgagagga ggtggcggtc
     1081 accgaggtcg gcggcgaggg tgtcgaggcg ttgttggtcg atgtcgcagg ctgcgatgcg
     1141 cgcgccttgt ttggcgaacg cgagcgcggt tgcgcgaccg atgccgctgc ctgcgcctgt
     1201 gatgacgacg acgcgatctt ggagctgcat gatttaggcg gcctcgggga cggggaatgg
     1261 gagaccacgc gcggcgaacc actcggcggc gaggccttcg ttggcgttgt cgttcgggtg
     1321 gaatgcgcgg cgcatgtatt ggcggatgcc tttgatgaag acgcgctgga tgatcggatc
     1381 gtctttgcgc atcttgcgaa gctcgcgcag cgtgccgcgc aagccgagct tttcttggcg
     1441 aaggagcatc gcggtgcccc acgcccagaa cgaggcgagc atgatcgtcg cgtaggcgag
     1501 gccggccatg cgaagggcgt aggagtcgtc gacttcggtg aggacgtcga atgcgaccgc
     1561 tttgtgctcg atctcttcgg ccgcgtgcca cgcgaggagc tgctgcatga gtgggtgcag
     1621 cgagtcgagg atgccccgct tgaacgcgcc ttcggcgagg atcgcggtga agtgttcggc
     1681 ggcggcggtg cctgcaaggt tgagcttggc cgggacgcgc ttctcgagcc acgtggagac
     1741 cgcgcggtag cggccgagga acgtgtcgat ctcgaagcct tgcgcgcgga ggactgcgtt
     1801 gaagtcgtcg tgcgctttgg cgtgatggcc ttcttgtttg aagaaggctt tgacgtgaga
     1861 gcggagtgtt gggtctttga tctgatcgaa gtacgcgttg acggagcgga cgaagaatcg
     1921 ctcgccgtgc gggaacagca ggttgatgcc gttcgagatc gccgttgccg cggcgttgcc
     1981 cgctagccag tggcgtggga ccttggtcag gtcgacgtcg agcgcgcggg gaatcgggat
     2041 cgacatagga tgaggttaga gcacgtattg accgtgagtc aatagtggtg ttgtatgatg
     2101 ggtactcaac gtgccgcctc gtcgtcccat ccgtcgcctc attcgcgctg ccgtcgcgcc
     2161 gcgcaagcgc cgtatccggc tcgacaatga cgagcgacgc gcgcagctcc tgacgctcgc
     2221 tcgcaaagcg ttcagcgatc gctcgtatga cgacgtgtcg atcgatgatc tcgcgcgcga
     2281 ggcgaagatc agcacggggc tgctctatca ctacttcccg acgaagcgcg atctgtatgt
     2341 agccgggttg cacgagattg ccgaggagct cgtcgagcgg tgtacggcga ttcccgcgaa
     2401 cctgccgccg atcgatcgcg tgcggacggg gctcgatgcg tatctcgatc acatctctca
     2461 gcatgcgcgc gcgtacgtgt cgctcatgcg cggcggtatt ggatcggatc ccgatgtcgc
     2521 cgcgatcgtc gaaggcgtgc gcaagcggct gaccgacaac ttcctcgagc agacgccgtt
     2581 tgccgcgatg ttggtgggcg acgtgcggtt tcagaccgcg gtgcgcgggt ggatcggctt
     2641 tgtcgaaggc gcgacgatcg attggtgcgc gaaccagcgc atgccgcgag aagatcttcg
     2701 cgagctgctg acgcagatct tgttcgcgat catgtcgacg gtcgccccgc aggtcatcaa
     2761 agccgctacc gtcagctgat gtcgcggtcg gtcgtgatca tcgatgggtt cgcgcccgag
     2821 gctcgtgcat tgcgcgcgac gttcgacgag cggtttgccg agccgcgcag cacgcgtggc
     2881 gatcgatttg tgtgggattg gtggcacgtg ccggggcagt acacggcgct gcgcacgccg
     2941 gcgtggacgt attttccgaa gaaggtctac gaagcgtttc atcgccggct cgtcgaatgg
     3001 ggccgcaaga cgcttggctg tcacgacatc tctccgccgt ggctgtcgat gtatgtcgat
     3061 ggttgcaggc aggagctgca cggcgatctg ccgcatgggc cgtgggcgtt cgtctactcg
     3121 ctgacgaatt ggaaaggccg cgtgtttcgc ggtggcgaga cgctgctcgt gcgcgacgag
     3181 gtcttggact tctggcacga cttccgatcg gtgcgtgccg tcgaggagcc cgagctcatt
     3241 cgtgcggtcg agccgcgctt cgatcggctg acggtgttcg acccccgcat cccgcatggc
     3301 gtgcgcgaag tgacgggcac acgcgacccg cgcgaaggcc gtctcgtcat tcacggttgg
     3361 ttcgtgcaac cgcggccgtt tattcgtgga ccgctgccga cgaagttgct cacgcagcgc
     3421 gcgaaggatt tgggcggcga gatcggcacg tggctcgacg agctcggcga catctcgatc
     3481 gccggcttgc tcgcagtggc gttcggcgtc gatcggcgcg gcaaggtcgg gcgcgtgcac
     3541 gtgctctccg atacgacgcg cgtgccgaag ctcgaggaaa agattcgcca gcagctgttg
     3601 cggcgaattc aggcgtcgat cagcggctgg acgttcggcg cgcagcgagc accgagcgcg
     3661 gtcacgctac cgctcgtgtt cgaacagggc ggctagaacc gatcccagac gtacgcgagc
     3721 tcggtgaccg ggaagccacc gacgcgcgtt tgcagccgcg agctctcgcg accgccaagc
     3781 gtttcgtaga agcgacgagc gtggtgattg ttgtcgagga cccacacgac catctggcga
     3841 attccgttgc gcgcgaagtg ggccatcacc tgctcgaaca tctcttggcc gagaccgtgt
     3901 cgttttgcgt gctgcacgag gtagatcgcg tagacctcgc ccgtcgcgtg cgtcggcagg
     3961 ctcggtaggg ggcggcgtga cgggcccgca tcgcagagcc cgacgatgtc gccatgcgtg
     4021 aggtcatacg cgacgagttg atagatcgat cggtcggaga tgatctggcg acgcgatgct
     4081 gcgcgctggc cgacgtcgat ccgatcgagc acggtgtcgg ggagaattcc gcgatagctc
     4141 gattgccagc tcgcggtgtg ggcacgcgcg atcgcctcgg catcgccgag cttcgcttca
     4201 cgaatgccga atctgctcac tcgctgactc cccagcgcag gagcttgcgg tagagcgtct
     4261 tgcgatcgag gccaaggatc ttcgctgcgt ctgtgcgatt ggagttgcac gcttcgagca
     4321 cgcgcagcac gtgccgccgt tcgacttcct cgagtgtgag cagctctggc acttcgccgc
     4381 cgctatgcgc gggtgagcga acgttctgtc gatcgcgaac tttctcgggg aggtcttcga
     4441 cctggatctc ttcgaagtgg gcgagcgcga cggcgcgttc gatgctgttg ctcagctctc
     4501 ggacgttgcc cggccagtcg tacgcgacga gccgctcggc ggccgcgggc gagataccga
     4561 tgaccttctt gccgaacacc gcggcgtact tgcgcaggac gtgctgggcg agcaagagga
     4621 cgtcgccgcc acgcgcgcga agcggcggca gcgggatgtg gatcacgttg atgcggtagt
     4681 agaggtcctc gcgaaaccgt ttgtcctcga ccatctcttc gagatcgtgg ctcgtcgcgg
     4741 cgacgaggcg gactttgtgc tcttggatcg cgcgcagaag cttggactgc agcgccggcg
     4801 ccatctcgcc gatctcgtcg aggaacagcg tgccgttggt ggcctgctgg aactgcccga
     4861 gcagctcggt gtcgagcgtc gcttcaggaa ctgctgcgca gttgaccgcg atgaacggtc
     4921 cggctttgcg acgactcttc tcgtggaccg cgcgggcgac gacttccttg cccgtgccgc
     4981 tctcgccggt gatgagcact gtggcgtcgg tggccgagac ttggtcgatc aagtcgtaca
     5041 cctgctgcat cgcggggctc gcgccgatga gatcaccgcg gccgcgcgtg ctcgcgacga
     5101 cctcgcgcag tcgcttcacc tcgccgcgca gctgtcgatg ctcggcggcg cgccggatcg
     5161 cgatcgcgag cgcctcgatc tcgacgggct tcgtgacgaa gtcgtacgcg ccggcgcgga
     5221 tcgcaccgac ggcggtctcg aagctgccga acgcagtgag cacgatgacg ggaatgtccg
     5281 ggcgatcggt ctcgatgcgc tggcagacgt cgaggccgtt catgcccttc atgttgagat
     5341 cggtgacgac gacatcgaca tcgtgctcgg tgagcgcggc gagtgcgtcg gcgcccgagt
     5401 tgaacggcac cacggagaag ccacgcttct tgaggccgag ctggatgagg tcgaccatct
     5461 cgcgctcgtc gtcgatgacg aagaccttca cgcggccgcc tccttggcgc gcgcgacatc
     5521 gcggggtaac cagacggtga acgtgctgcc ggcgcctttc tc
//
LOCUS       DATLBZ010000394         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10669160, whole genome shotgun sequence.
ACCESSION   DATLBZ010000394 DATLBZ010000000
VERSION     DATLBZ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10669160"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VMZ53_20010"
     CDS             complement(<1..1149)
                     /locus_tag="VMZ53_20010"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30811.1"
                     /translation="MTRYELLDRIGVGGMAEIFRGKAVAAGGFEKPVAIKRILPHLSQ
                     DRRFVELLIAEAKVLSLLKHRNIVQIFDVGLGDDGQYFLVMEYVDGKDLGGVQRALES
                     NRKRLPFDLALHIGAEICEALEHAHVSRGPDGKPMALVHRDVSPSNVLLSRAGEVKLT
                     DFGIAKRAESDATQGGAVRGKFAYISPEQARNETLDPRSDVFSVGILMWELCCNRRLF
                     SGLDDLAALRAVRDPIVKRPSEIDPRLPPELDQLLMTALARDKKQRFASCGALGQKLR
                     SLRYSLDVTVGDPATELAKVIDSAEEVERQSKQHIAVVPSGRHDFDGAEATVIRIHTA
                     DAFSARDEGKSISQARQVIDRFEEEETRLASLSGDQLRMLRKGAQRDSV"
     gene            1217..>2123
                     /locus_tag="VMZ53_20015"
     CDS             1217..>2123
                     /locus_tag="VMZ53_20015"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUS30812.1"
                     /translation="MQRIRALLDDALAAGLGSAAAVSVGDSGREVFRYFAGTTRRVPD
                     RGPAITEATPFDLASVTKPMSTVAIAMVLVAEGRLDVDAPVRRYLPAAQTTGTVRHLL
                     GHTAGCASHVEFFRRLRAERPADPRSRLVELACAEPCSAPGVTAVYSDLGFIQLGAVV
                     EAAAAMPLERAFTEYVAGPLGLAAARFAPTPIDDAVSTELDDRGVVTGLVHDENCYFG
                     GRIAGHAGLFAPLGDVARFAQAIVDTAAGTPHGRFTTDVVTRFFTDAPTPGSPWRLGW
                     DAPSHTPGVSQAGDRWPRTGAVGHTG"
BASE COUNT          315 a          746 c          696 g          366 t
ORIGIN      
        1 gacggagtcg cgctgcgcgc ctttgcgcag catgcggagc tgatcgccgg agagcgatgc
       61 gagccgggtc tcctcttctt cgaagcgatc gatcacctga cgcgcttgcg agatgctctt
      121 gccctcgtcg cgcgcgctga aagcatccgc cgtgtgaatg cggatgacgg tggcttccgc
      181 gccgtcgaag tcgtggcgcc ccgacggcac gaccgcgatg tgctgcttgc tctggcgctc
      241 gacttcctct gccgagtcga tcaccttcgc gagctcggtc gccggatctc cgacggtgac
      301 gtcgagcgag tagcgcagcg agcgcagctt ctgaccgagc gcgccgcagc tcgcgaagcg
      361 ttgcttcttg tcgcgcgcga gtgccgtcat caagagctgg tcgagctctg gcggcaagcg
      421 cggatcgatc tcgctcggcc gcttcacgat cgggtcgcgc acagcacgca gcgcggcgag
      481 atcatcgagg cccgagaaca ggcggcgatt gcagcagagc tcccacatca ggatgccgac
      541 ggagaacacg tcgctgcgtg gatcgagcgt ctcgttgcgc gcttgctccg gcgagatgta
      601 cgcgaacttg ccgcgcaccg ccccgccttg cgtcgcgtca gactccgcgc gcttcgcgat
      661 gccgaagtcc gtgagcttca cttcgcccgc gcgcgacagc agcacgttcg acggcgacac
      721 atcgcgatgc acgagcgcca tcggtttgcc gtcgggaccg cgcgagacgt gcgcatgctc
      781 gagcgcttcg cagatctctg cgccgatgtg gagcgcgaga tcgaacggca accgtttccg
      841 attgctctcg agtgcacgct gcacgccgcc gaggtccttg ccgtcgacgt actccatcac
      901 gaggaagtac tgaccgtcgt cgcccaatcc gacgtcgaag atctggacga tgttgcgatg
      961 cttgagcagc gacaacacct tggcctcggc gatcagcagc tcgacgaacc gccggtcctg
     1021 actcaggtgc ggcaggattc gcttgatcgc gacgggcttc tcgaagccgc cggcggccac
     1081 ggccttgccg cggaaaatct ccgccatgcc gccgacgcca atgcggtcga gcagctcgta
     1141 ccgagtcacg tggggtcagc ctagcatggg gcgtgggcgc gatatgatga cgtagccgtc
     1201 gatgatcatg tcctacatgc agcgcatacg ggcacttctc gacgacgcgc tcgccgctgg
     1261 cctcggctcg gccgccgcgg tctccgtcgg tgattccggc cgcgaggtgt tccgctattt
     1321 cgccggtacg acgcgccgcg ttccggaccg aggtccggca atcacggagg ccactccgtt
     1381 cgatctcgcg agcgtgacca agccgatgtc gaccgtcgcg atcgcgatgg tgctcgtcgc
     1441 cgaaggccgt ctcgatgtcg acgcgccggt gcgccgctac ctgcccgccg cgcagacgac
     1501 cggtaccgtg cgacatctcc taggtcacac cgctgggtgc gcctcgcacg tcgaattctt
     1561 ccgtcgcctc cgcgccgagc gtcccgcgga tccacgcagt cgtctcgtcg agcttgcatg
     1621 tgcagagccg tgcagcgcgc ccggcgtcac cgcggtctac agcgacctcg gctttatcca
     1681 gctcggtgcc gtcgtcgaag ccgccgctgc gatgccgctc gagcgcgcgt tcaccgagta
     1741 cgtcgccggt ccgctcgggc tcgctgccgc gcggttcgcg ccaacgccga tcgacgacgc
     1801 ggtctcgacg gagctcgacg atcggggcgt cgtcacgggg ctcgtccacg acgagaactg
     1861 ctacttcggc ggacgcatcg caggacacgc gggcctgttc gcgccgctcg gtgatgtcgc
     1921 gcggttcgcg caggcgatcg tcgacaccgc cgccggcaca ccgcacggcc gcttcacgac
     1981 agacgtcgtc acgcgcttct tcaccgatgc gccgacacct ggctcaccgt ggcgcctcgg
     2041 ttgggatgcg ccgtcgcaca cgccgggagt ttcgcaggcc ggcgatcgct ggccacgcac
     2101 gggcgccgtc ggtcacaccg gct
//
LOCUS       DATLBZ010000395         5040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4935809, whole genome shotgun sequence.
ACCESSION   DATLBZ010000395 DATLBZ010000000
VERSION     DATLBZ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4935809"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VMZ53_20020"
     CDS             <1..477
                     /locus_tag="VMZ53_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30813.1"
                     /translation="VGTSESSSTGTEPDAAVQGMTPDAAVSSPDAPSGAFQCRDKQPL
                     TAVGDGHHHPGENCLNACHNHGFTLAGTMTTTATATTPFVGASITVKDANGVTFDMVT
                     QANGNFYTRAVVTFPVTVTASACPSIHAMSGTVTAGGCNKTGCHSATGGAGHITLP"
     gene            complement(518..1204)
                     /locus_tag="VMZ53_20025"
     CDS             complement(518..1204)
                     /locus_tag="VMZ53_20025"
                     /inference="COORDINATES: protein motif:HMM:NF037025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HUS30814.1"
                     /translation="MPTDDDLPPLQSEAAFGNNPRTAFNFFVSKGLTQVQAAGVVGNL
                     MQESSVNPAAVELGGGPGRGIAQWSVGGRFNVGSKSLTSFAAARGLSKWALNTQLDFI
                     WYELATVGGYGLADLRAATTLNQAVYAFQAKYEICGKCAAGKRLTYAQEVLAQYGGST
                     STGTGTGTGTTPPPPPTMETCYSGTLDREMPENACVESMYDGLWYQCSSGQWVDRWSD
                     PDACNGEYPL"
     gene            complement(1387..1515)
                     /locus_tag="VMZ53_20030"
     CDS             complement(1387..1515)
                     /locus_tag="VMZ53_20030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30815.1"
                     /translation="MRFDATAIVTRVRVVIGAVARFAPLVARFVTSAVISPHWKID"
     gene            complement(1590..2513)
                     /locus_tag="VMZ53_20035"
     CDS             complement(1590..2513)
                     /locus_tag="VMZ53_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30816.1"
                     /translation="MRFVAALVVVLAVAGSSVHADPAADAKAKVAADAGAALFGKNDF
                     AGAAKKFREAYDLNHDASYLFNIAQAYRHAGDCIRSADYYGRFLAVVPHPPNEDKIRV
                     WYASELQCAKERAATVPVDDKQTHDATNRDATNGDRNATNHDATNGDRNATNGDANHD
                     ATNGDRKATHGGANHGGTNGDAATQQSSDASSGGGHRGLALALGGVGVVALGVGGFFA
                     WDASYLSDRRASFLAGCGTTDRCSSAVVNDYDRRGSRAQTLAIVGFAAGGVAIAASAT
                     LYLMAGRSEAPPAVAITHVEGGAIVTRAFAW"
     gene            complement(2538..4337)
                     /locus_tag="VMZ53_20040"
     CDS             complement(2538..4337)
                     /locus_tag="VMZ53_20040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30817.1"
                     /translation="MRCPDENVLAEYVDNVLDAAERRSVEQHLDTCAACRTVVSDLAR
                     MQHAAGDGDEATLAEALGSHTTIDAVRHRTRTAHILPGTMIGEYEVRGVLGAGGMGIV
                     FAAVHPRIGKRAAIKILQRDLALDHSSLVRFENEARAVNEIGHPNIVDIFSFGELPNG
                     DPYYVMEHVDGVSLHDWISSHGPVSLDRAMPILRQICDALTAAHERGIVHRDLKPGNI
                     MVAGSDDAPRVKVLDFGLAKMVRRDYDDELTHPGIAIGTPAFMSPEQFMGREVDQRTD
                     VYALGVVVYQMLTGAYPFQGETPSAIGEQHLYHTPAKPSALANLPERVDHIVSKALAK
                     NADERYATVKDFRAELDRCAAENPKPRAASWPASPPAMSDAPTMVPVAAAAMPVESAT
                     SMRPTTPHQVEEPSETSMRPTTPHQAEEPTETSMRPTTPMPFAEPAAATMRRRWIVGA
                     VATALVALVVVMVATNGPSKSTSARVDPQAAPAPGAHVASPVASPVASPVASPVADEG
                     AALPVGQAARPGVADTNPAAVADTKVKATSPTTSSRAKATSATSRAKATSATTTSRAK
                     ATSATTTSSAGTGSASADDDRMLLEGGAVKPPK"
     gene            4419..>5040
                     /locus_tag="VMZ53_20045"
     CDS             4419..>5040
                     /locus_tag="VMZ53_20045"
                     /inference="COORDINATES: protein motif:HMM:NF013457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HUS30818.1"
                     /translation="MTFSRIALCSFGLLAACAQQPGDKPDPVEESDDPIDQVIAKMRP
                     LPVDPPMLEKGTPSAPMTDGDYQCVDTPIKEVRQYDQLLGQLNIGDVMWPGAMLRGDS
                     VYAGRLTPIALARAPMTFSVSLESLGDGRHAATLDNPSLSSYRDAVGKILAQSLSGST
                     AARMSSEVDEVNSEKELALALGVDATAPLVGSIKAGFNFSDSTKRSR"
BASE COUNT          800 a         1722 c         1647 g          871 t
ORIGIN      
        1 gtcggcacgt cggaatcgtc atcgacgggg accgagcccg acgcagcggt gcaggggatg
       61 acgcccgacg cggccgtcag ctcgcccgat gcaccgagcg gtgcatttca atgtcgcgac
      121 aagcagccac tgactgcggt cggtgatggc catcatcatc ccggcgagaa ctgcctcaac
      181 gcgtgccaca accatggctt cacgctcgcg ggaacgatga cgaccaccgc gactgcgacc
      241 acgccgttcg tcggtgcgtc gatcacggtg aaggacgcga atggcgtgac gttcgacatg
      301 gtcacgcagg caaacggcaa cttctacacg cgggcggtcg tgacgttccc cgtgaccgtc
      361 accgcgagtg cgtgcccgag catccatgcg atgagcggca ccgtgaccgc gggcggctgc
      421 aacaagaccg gttgccactc cgcgacgggc ggcgctggcc acatcacgct gccctgagct
      481 tcgtcgcggc gctggcagac agcgcgcgta tcgctgatca gagcgggtac tcgccgttgc
      541 acgcgtccgg atccgaccag cggtcgaccc actggccgct cgagcactga taccagaggc
      601 cgtcgtacat gctctcgacg cacgcgttct ccggcatctc gcgatcgagc gtgcccgagt
      661 agcaggtctc catcgtcggc gggggtggcg gcgtcgtgcc ggtgcccgta cccgtgccgg
      721 tgctcgtgct gccgccgtat tgcgcgagca cttcctgcgc gtacgtgagc cgcttgccgg
      781 ccgcgcactt gccgcagatt tcgtacttcg cctggaacgc gtagaccgcc tggttcagcg
      841 tcgtcgccgc gcgcaggtcc gccagtccgt agccgccgac cgtcgcgagc tcgtaccaga
      901 tgaaatcgag ctgcgtgttg agcgcccact tcgacaggcc gcgcgcggcg gcgaacgacg
      961 tgaggctctt gctgccaacg ttgaagcgac cgccgaccga ccactgcgcg atgccgcgac
     1021 cgggaccacc accgagctcg acggcagccg ggttgacgct cgactcctgc atgagattgc
     1081 cgaccacacc ggcggcctgc acctgcgtga gtcccttcga cacgaagaag ttgaacgcgg
     1141 tgcgcgggtt gttcccgaac gctgcctccg attgcagcgg cggcagatca tcatcggtag
     1201 gcatcgttgg atcaccaacc atgcaggccg aggccaacat ggcgaccacg atcgcgagac
     1261 gtgttccgtg catcatcggc cagacgcttt cgcaagctgc atgccccgcg tcgaccctgg
     1321 attatcggct gcttacgccg gcacccattg cgactccacc ccccatgggc tgactccact
     1381 ccctggctaa tcgatcttcc agtgcggcga gatcacagcg gaagtcacga atcgcgccac
     1441 gagcggcgcg aaacgcgcca ccgcgccgat caccacgcga acgcgcgtga cgatcgccgt
     1501 ggcgtcgaac ctcaccaccc gtgacgatcg caccgccttc gcggatcccc acgcgaacgc
     1561 gcgcgtgacg atcgcaccgc cttcgcagat caccacgcga acgcgcgcgt gacgatcgcg
     1621 ccgccttcga catgcgtgat cgcgacggcc ggtggcgcct cgctacgccc cgccatcagg
     1681 tagagcgtcg cgctcgcggc gatcgcgaca ccgcccgcag cgaagccgac gatcgccagt
     1741 gtctgtgcac gcgagccacg acgatcgtag tcgttgacga ccgccgatga gcagcgatcg
     1801 gtcgtgccgc agcccgcgag aaacgacgcg cgacgatccg acaggtagct cgcatcccac
     1861 gcgaagaagc caccgactcc gagcgcgaca acgcccacgc cgccgagcgc aagcgcgagg
     1921 ccgcgatgcc cgccacccga gctcgcgtcg gaagattgct gcgtcgccgc gtcgccgttc
     1981 gtcccgccgt ggttcgcgcc gccgtgcgtc gctttacggt cgccgttcgt cgcgtcgtgg
     2041 ttcgcgtcgc cgttcgtcgc gttacggtcg ccgttcgtcg cgtcgtggtt cgtcgcgtta
     2101 cggtctccgt tcgtcgcgtc gcggttcgtc gcgtcgtgcg tctgcttgtc gtcgacgggc
     2161 acggtcgcgg cgcgttcctt cgcgcactgc agctcggacg cgtaccagac gcggatcttg
     2221 tcctcgttcg gcggatgcgg aacgactgcc aggaagcggc cgtagtaatc ggcggacctg
     2281 atgcagtcac cggcgtggcg atacgcctgc gcgatgttga acaggtagct ggcgtcatgg
     2341 ttgaggtcgt acgcctcgcg gaacttcttc gccgcgccag cgaagtcgtt cttgccgaac
     2401 agcgccgcgc ctgcatcggc cgcgactttc gcctttgcgt ccgccgccgg atccgcatgc
     2461 accgacgatc cggcgaccgc aagcacgacg accaatgcag ccacgaacct cacagcacca
     2521 gcttacagca cacgtggtta cttcggcggc ttcacagcgc cgccctcgag caacatgcgg
     2581 tcatcgtcgg cgcttgccga gccagtgcca gctgaagagg tcgtcgtcgc gcttgtcgcc
     2641 tttgcacgcg atgttgtcgt cgcgcttgtc gcctttgcac gcgatgtcgc gcttgtcgcc
     2701 ttcgcgcgcg agcttgtcgt cgggctcgtc gccttcacct tcgtatccgc gacggccgcg
     2761 gggttcgtat cggcgacacc gggtcgagcg gcttgcccga caggcagggc cgcgccttca
     2821 tcagcgacgg gcgacgcgac gggcgacgcg acgggcgacg cgacgggcga cgcgacatga
     2881 gcgcctggag ccggcgccgc ctgcggatcg acgcgtgcgc ttgtcgactt gctcggaccg
     2941 ttcgtcgcga ccatcacgac gacgagcgcg accaacgcag tcgcgaccgc gccgacgatc
     3001 caccgccggc gcatcgtcgc cgcggcgggt tcggcaaacg gcatcggcgt cgtcggccgc
     3061 atcgacgtct cagtcggctc ctcggcctga tggggcgtcg tcggccgcat cgacgtctca
     3121 ctcggctcct cgacctgatg gggcgtcgtc ggccgcatcg acgtcgcgct ctcgacgggc
     3181 atcgccgctg ccgcgacagg gaccatcgtc ggtgcgtcgg acatggcggg cggtgatgca
     3241 ggccacgacg cggctcgcgg ctttgggttc tcggcggcgc agcgatcgag ctccgcgcgg
     3301 aagtccttca ccgttgcgta gcgctcgtcg gcattcttcg cgagcgcttt gctcacgatg
     3361 tggtcgacgc gctctggcag gttggccagc gccgacggct ttgcgggcgt gtgatagagg
     3421 tgctgctcac caatcgccga tggcgtttcg ccctggaatg ggtacgcgcc cgtcagcatc
     3481 tggtagacca cgacgccgag cgcgtagaca tccgtccgtt gatcgacctc gcgacccatg
     3541 aactgctcgg gcgacatgaa tgccggcgtg ccgatcgcga tgcccgggtg cgtgagctcg
     3601 tcgtcgtaat cgcggcggac catctttgcg agaccgaagt cgagcacttt gacgcgcggt
     3661 gcgtcgtcgc tgcctgcgac catgatgttg ccgggcttga ggtcgcgatg gacgatgccg
     3721 cgttcgtggg cggcagtgag cgcatcgcag atctgtcgca agatcggcat cgcgcgatcg
     3781 agcgagaccg ggccgtgcga cgagatccag tcgtgcaggc tcacgccgtc gacgtgctcc
     3841 atcacgtaat acggatcgcc gttcggcagc tcgccgaagc tgaagatgtc gacgatgttc
     3901 gggtgaccga tctcgttcac tgcgcgggct tcgttctcga agcgcacgag cgagctgtga
     3961 tcgagcgcga ggtcgcgctg gaggatcttg atcgcggcgc gcttgccgat gcgtggatgc
     4021 acagcggcga acacgatgcc catgccgcct gcgccgagca cgcctctgac ttcgtactcg
     4081 ccgatcatcg tgcccggcag gatgtgcgct gtccgcgtgc gatggcgaac cgcatcgatc
     4141 gtcgtgtgcg agccgagggc ttcggcgagc gttgcttcgt caccgtcgcc tgcggcgtgt
     4201 tgcatgcgcg cgagatcgga gacgaccgtg cggcacgcag cacaggtgtc gagatgttgc
     4261 tcgacggacc gtcgctccgc cgcgtcgagc acgttgtcga cgtactcggc gagcacgttt
     4321 tcgtccggac acctcacccg tttcaatatc ttaccggata gttctgcgac attcggcggg
     4381 cgctgcaact agggggaaat ccaacctgga gacctcccat gaccttctcg cgcatcgccc
     4441 tgtgttcgtt cggacttctc gccgcgtgtg cccagcagcc tggcgacaaa cctgaccccg
     4501 tcgaagaatc ggacgatccg atcgatcaag tgatcgcgaa gatgcggccg ctgccggtcg
     4561 acccgccgat gctcgagaaa gggacgccga gcgctcccat gacggacggc gactaccagt
     4621 gcgtcgacac gccgatcaag gaagtgcgtc agtacgacca gctgctcggc cagctcaaca
     4681 tcggtgatgt catgtggccg ggtgcgatgc tgcgtggcga ctccgtctac gctggcaggc
     4741 tgacgccgat cgcacttgct cgcgcaccca tgacgttctc ggtgtcgctc gagagcctcg
     4801 gggatggcag gcacgctgcg acgctcgata acccgagcct gtcgagttat cgcgacgcgg
     4861 tcggcaagat cctcgcgcag tcactgagcg gatcgaccgc agctcgcatg tcgtcggaag
     4921 tcgacgaggt gaattccgag aaagagctcg cgctcgccct cggtgtcgac gcgacggctc
     4981 cgctcgttgg cagcatcaag gcgggcttca acttcagtga ctcgacgaag cgcagccgct
//
LOCUS       DATLBZ010000396        15088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9925138, whole genome shotgun sequence.
ACCESSION   DATLBZ010000396 DATLBZ010000000
VERSION     DATLBZ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15088
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9925138"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VMZ53_20050"
     CDS             <1..723
                     /locus_tag="VMZ53_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30819.1"
                     /translation="REDWMRRLGPLYGGAVALIHSTPWRWPETVSVGNEARSPFWVVA
                     IGFPIGLVAYVVAALLDAVGLPATICAFAGLATLTLASAAIVEKGLVARIDGSESTSP
                     SVPAIVGLVFAILIRAAAIAAVSPDHWLGLFIAAALAGRWAAIFLQALGDPIIDDDTR
                     RSLVATPAPAWLTAAISIGVATLVIIALGKRGIVALALTAAIAFGLGLDAQRRDRGLS
                     SAVVATAAAIGELAILLVAALR"
     gene            complement(724..2178)
                     /locus_tag="VMZ53_20055"
     CDS             complement(724..2178)
                     /locus_tag="VMZ53_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30820.1"
                     /translation="MSKWLTLAAVLCACQSKNTDKPAKPATGSGSGSIEVVATGSGSA
                     KDPWVTVDAAPVTPESKRKAAEAAVSRVESIEPKLAKLRELPLKKEVPAAYQTTDDFR
                     AFLKRDIDKELPPKKSKDMGDALLHIGLLDKPVDLADTLIKTMVTQAAAYYDPAQKKF
                     FVVMAPDNEMLLDTMSAHELTHALQDQHFDLAKYLPSGLDDDQTNARHFVVEGDATFA
                     MLVYTSASGGSASKETTNLLMNLVKTQVEAMAAMDISAFADQMKQQANAFGQMDDDMR
                     KSMESIGELPLAIIGPLIDSYMKGAVLCMAAYEHGGWKGVDDLYKNPPESTEQVLHPS
                     TKMYPTREHPRAITLPKMEGTEVETNVMGELQWSNYFQVWKLKVPDAAPGWGGDKFSV
                     TRDKDGHLLGVLVSAWDSEADAKQFVDAYVQALAARFPGAKGDALKDGLPRPDFGKVF
                     VRQVGDRVYIVDGAADVKVLDRVVKDAKVAPAKK"
     gene            complement(2191..2460)
                     /locus_tag="VMZ53_20060"
     CDS             complement(2191..2460)
                     /locus_tag="VMZ53_20060"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HUS30821.1"
                     /translation="MDDEEVFTPELTDQLDLHTFQPRECADVVDEYLTAAQEAGFTTV
                     RIIHGKGTGTLRRIVQGVLERHSAVASFEQADANWGATRVVLKSS"
     gene            2482..4101
                     /locus_tag="VMZ53_20065"
     CDS             2482..4101
                     /locus_tag="VMZ53_20065"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUS30822.1"
                     /translation="MRFVAVVVAVVACSRPLSTNTSTHAHLLLVNGHIFTGDPAQPWT
                     DTIAIGGDEIVAIGDEAKRFTANRRIDLGGRLVIPGINDAHVHEPNLLPFVDVAEGFD
                     KLTVADLLAAFEKAEHEQPAGTWLRGDLSQAALDDPTLTKATLDRVAPTHPVWLDNFA
                     GHVVVMNSAAEKLLGLDHNAPHAGFLGREDSGADDGWRYEYARYEAKRRIGLELSDAD
                     IARAMHDFEQQATTFGITTVQTFPLDIEAERLVIAGNVTRHAVRWHVIRFPLGSVIDP
                     PRASPPDASSRLYLSGTKYILDGTPVERGAALLGPYHDRAGWEGRVDWSREEVRHMLV
                     AARASGDALHVHVAGDRSVVLVLDLMQELGGDWRASRVVLEHGDGLAAADFARAQQLG
                     VIVVQNPIHSATAELNHARLGDRALHWMPMRSLLAARIPFALGSDGPLNPFLNIMMAE
                     MHPTNHGEALTREQAVIAYTQGAAFDERLEQRKGKLVPGMVADIAVLSQDIFKLPADA
                     LPATRSVLTIVGGEVVYDASRASAPTSARTE"
     gene            complement(4061..4912)
                     /locus_tag="VMZ53_20070"
     CDS             complement(4061..4912)
                     /locus_tag="VMZ53_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS30823.1"
                     /translation="MLTTVAAGPFTIRGVSVGGVYTSLAVPELGLVFDAGIAPRSAGG
                     IDTILLSHGHADHVGALPALLGIRALHGKQKPPRVVMPAEIVDDLRVALGALSKLQRW
                     PLEVEAIGMLPGDEIELRGDLRVRAVRTFHPVPSLAYIIVRRVTKLREEFRGLPGPEI
                     AARRQAGEPMLDVDERLELAYATDTLVSVLDHSPELLKTRVLIMECTFLDSRKTLEAA
                     RAGCHIHLDEVIERAEKFENEHIVMMHLSQLYRPDEVASILDARVPPSLRSRIIPFVP
                     KSGHWPG"
     gene            4954..6354
                     /locus_tag="VMZ53_20075"
     CDS             4954..6354
                     /locus_tag="VMZ53_20075"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HUS30824.1"
                     /translation="MLYALLAVVAVLLLLSLALPVPRRIWAFSLYTTKYFFLWLSDVL
                     MLRRLWFLITGRGKKYQKLTRPMILRMFCEDLGPTFIKFGQIIASSAGMFPDAYVKEF
                     QRVLDRVKPFPFEDVKRTLVEDLGAEKAAHLVDIEPKQLASASIAQVHTAKLRDGTPV
                     VIKVQRPGIIARCMADMKIMRFMAKIVAKLRKNVELANPVGVVDDFTKTLSEELDFRK
                     EAANLDQFNMIMKELGYKHIHAPVPHHEYTTRKVLVMTKFVGLRVDKFDEIKARGIDG
                     ETKLVDGLRAWFQCVVFYGFFHGDVHAGNLMLLDDDTIGFLDFGIVGRFNDEERYLVT
                     DYMIAFASGNYKRLAEIIIEMAGAPDGLDMEAFVKDLGDTYRPLLTLSFGEVNYAEFL
                     PGIQRTATRHKMRMPNEFILITKQLLYFDRYAKSLAPKLNVFTDPRLVLGMMSDIQKA
                     RMQADAAKKAKSSATT"
     gene            complement(6351..7772)
                     /locus_tag="VMZ53_20080"
     CDS             complement(6351..7772)
                     /locus_tag="VMZ53_20080"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS30825.1"
                     /translation="MSSSTTLDSKTASEVTVRDSEPTVAALVIVWSATDPARVGEVIV
                     PRADGEPAVFGRGPREGDEPRAELVRQRPGGLEATVPIDNPFLSRRHLAIAAESGGIA
                     IENFGKKALLLDGEAADSELVVRIGETVEIPGQLMFLCVDRPRTLPQSHAKAGTFGEP
                     DAHGIVGESPLAWELREQCARVGALSAHVLLLGESGTGKELAAQAIHAHSTRSAKKLV
                     ARNAATFPPGLIDAELFGNLASYPNAGMPERPGVIGEADGSTLFLDEIAEMPQELQSH
                     LLRVLDAGEYSRLGEAKRRTADLRVVAATNRGADALKHDLAARLALRITLPSLNARRE
                     DIPLIARHLLRRHARKDAPIGARFLAGWDGTTGEPRLTPALVRALVGHKYTTNVRELD
                     SYLLRSLVASRGSSVELPESLASELVRAHKPRLAAQPYSAEQIQAALAKHDGVREKVW
                     RELGMANRYVLKRLMKKYGIADD"
     gene            complement(7834..11052)
                     /locus_tag="VMZ53_20085"
     CDS             complement(7834..11052)
                     /locus_tag="VMZ53_20085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30826.1"
                     /translation="MTRTLSQTMVETNPGTVLAGKYRVERVLGTGGMGVVVAALHEQL
                     DERVAIKLLRADRLAQADAVERALREARATVKIQSDHVVRVFDVGTLENGSPYIVMEY
                     LAGCDLAQLLCAEGKLDVADACSYVRQACDAVGKAHAIGIIHRDLKPANMFLAVRDDK
                     SAIKVLDFGISKFVSTDSHRVSPSLTSTASVLGSPAYMSPEQLESPREIDARTDIWGL
                     GVILFELLTGAHPFVADTLPQLYKKIADEPAPSVKSIRPDVPVDLDAIVARCLAKKPA
                     DRFADVASLARVLAPFAVDASDERKLAGLAETASASREEIESARLASKISGPAIANID
                     APLATANSVAAANAVAAGASANASGVGTSTSEGRGQIEARGHSDARGQIEARAGISMG
                     VERTQARRRRLAWSGFAFAGLAALATSTLVCVRADSQRRTASAKGDAGNGAAGGAAGR
                     MATSPLKAAGSVLACPPLVASGVEQPAGWLGAAAASIACTRATVRMGNRAERTLLPAE
                     LLDLPRTVVDNFPEDAFSAADARERAIAAAKTRGQAWLDGSIDHTADGFTVELVLRVG
                     DRELAHGSGRSATLVPAIHDAMAPIDRFVPAARDDDPFLREWMGARTADAAVAIAEWS
                     IGNRLEWPNKSGCEALSKNPDILPITQKAIHNWCIPEPQDYPDRDTELPGTLVASITA
                     DGQLSKRPLDAELARIEKALAITKDRQARAFLLAAKSEIFTGHRMYPEATQAALESIG
                     ESADAFFPWTTGWNAVAWTRANSNAAPASLAWTPWNVEVYCFAATRAAKTDDGIAYMR
                     RNTVLSHGSIWKEALVEFLVHGNKVDEARSLAAKLPTDPVNLVVDAAEGHFAQATERA
                     EMQMKDKDPEVAVNAAYYMSDLAVVTGKPARGTQELIERFVDKHPEQVKGNVLIEINV
                     ALVCMKAQRDIAKHCFQRLREMTGGVVFFDVNFLDGAERYSQGDFAGAAKAWRALLAR
                     PDWHLFFMHDTIADAFERGNDPENAARVDARYLRDGRFNGAEMAHVREARRAEKRGDA
                     AQARAMAKKVIDAWSVVDVDVPAVAEMRQLIARLDKKPATSH"
     gene            complement(11168..11563)
                     /locus_tag="VMZ53_20090"
     CDS             complement(11168..11563)
                     /locus_tag="VMZ53_20090"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS30827.1"
                     /translation="MSGSVERQHPRYAHAVAVTIHFGKRTTAGRTTNVSRGGLCADVE
                     AEIKAGSDVEVDIVLVFDDEMQSEALRLPARVAWCTTVDNAFQVGVSFKPLDTERSKY
                     LQLFLKYLGDERAAKQPRSSSLDVDKRFG"
     gene            complement(11682..12476)
                     /locus_tag="VMZ53_20095"
     CDS             complement(11682..12476)
                     /locus_tag="VMZ53_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30828.1"
                     /translation="MAVVGLLVALAARAHASPADRRGIERGSQRGGAGGLHDAIPDGT
                     DRGFDDAIDGDDGDDDVSVDLPYAVGGSAGADRGRRAGIDALCDCDAPTLSRIAATAP
                     ELSTIIARAQSIAGLAGADAPTASWRRRSRWSALIPVISLRVGNSQSWRDILDPAVAA
                     SSSSSAPTLNHSLSYDARVAWHLDHLLYDPNEPRFTAFELSRRRERRLLAAVTSRAYF
                     VWLRASVAVERDGRWELRVAEAAAELDALTDGWFSENRGANRRESR"
     gene            12576..14846
                     /locus_tag="VMZ53_20100"
     CDS             12576..14846
                     /locus_tag="VMZ53_20100"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HUS30829.1"
                     /translation="MTATLTDNALQVLRARYLARENGIVVETPDQLFRRVARHIAAVE
                     SGLGYSEAEVEGARAKFERMMSSLEFVPNSPTLMNAGRPLGQLAACFVVPVDDSTTGV
                     FEAVRWAAEIQKTGGGTGFSFSRLRPAGDLVASTGGNASGPVSLMQVFDVATEAIKQG
                     GTRRGANMGMLRVDHPDVLEFIALKLDPTRMRNFNLSVAITDAFMAAVAAGTTYDLIN
                     PRTKVVTGQLDARRVFDAIANAAWAVGDPGLVFIDRVNELHPTPALGAIEATNPCVVG
                     GTLVWTEERGLVAIEQLVGERPRVATLDGERVVFREASQVIATGVKPVFRLINDRGLH
                     LICTADHRITTSRGDVAAQDLVVGDTIRVVAGGRSDLASGARLDLAAGSLQTHLGWAT
                     FRGLEPLGDAPVFDLTEPTTSHFFADGVLVHNCGEQPLLPFESCTLGSIDLGRFVHGG
                     DFDWERLRECIHDAVRFLDDVIDANRYPLLEIERATKATRKIGLGFMGWADALVLLDI
                     AYDSDAAIELADRVAAFLETESLAASAALADRRGAFPSWDRSRWQTAGHRPLRNATTT
                     TIAPTGTISIIAGCASGIEPLYALAYRRNVLDGAELTEINPAFQRIASERGFASPELF
                     TTVAEHGGVRGNDAVPTDVQRRFPTAHDVSVDMHVRMQAAFQRHVHAAVSKTINLRRE
                     ASAADVKAAYQLAYELGCKGITVYRDGSREGQVLVTGARGPVVSATPSCPECGSVLVV
                     QTHCRLCRHCGWSVCG"
BASE COUNT         2413 a         5181 c         4817 g         2677 t
ORIGIN      
        1 cgcgaggact ggatgcgccg cctcggcccg ctgtatggcg gcgcggtcgc gctgatccat
       61 tcaacgccgt ggcgctggcc cgagacggta tcggtgggca acgaggcacg ctcgccattc
      121 tgggtcgtcg cgatcgggtt cccgatcggt ctcgtcgcgt acgtcgttgc tgcgctgctc
      181 gatgccgtcg ggctcccggc cacgatctgc gcgttcgcag gtcttgcgac gctgacgctg
      241 gcatcggccg cgatcgtcga gaagggcctc gtcgcgcgca tcgatggatc cgagtcgacc
      301 tcgccgagcg tgccggccat cgtcgggctc gtcttcgcga tcctgattcg cgcggccgcg
      361 atcgcggcgg tctctcccga tcattggctc ggcctgttca tcgcagcggc gctcgcaggc
      421 cgttgggccg ccatctttct gcaggcgctc ggcgatccga tcatcgatga cgacacgcgc
      481 cgctcgctcg ttgccacgcc cgcaccggca tggctcacgg ccgcgatcag catcggcgtc
      541 gcgacgctcg tcatcatcgc gctaggcaag cgaggcatcg tcgcgctcgc tctcaccgcc
      601 gccatcgcgt tcggcctcgg cctcgatgcg cagcgccgcg atcgcggact gtcatcggcc
      661 gtcgtcgcga ccgcagcagc gatcggcgag ctcgcgattc tgctggtcgc cgcgctccgc
      721 tagctacttc ttcgcgggtg cgaccttcgc atctttgacg acgcggtcga gcaccttcac
      781 gtcggccgca ccgtcgacga tgtacacgcg atcgccgacc tgccgcacga acaccttgcc
      841 gaagtcggga cgcgggaggc cgtccttcag cgcatcgccc ttcgcaccgg ggaaccgagc
      901 ggcgagcgcc tgtacgtacg cgtcgacgaa ctgcttcgcg tcggcttcgc tgtcccacgc
      961 gctgacgagc acgccgagga ggtggccatc cttatcgcgc gtcaccgaga acttgtcgcc
     1021 accccaacct ggcgcagcgt cgggcacctt gagcttccac acctggaagt agttcgacca
     1081 ctggagctcg cccatcacgt tcgtctcgac ctcggtgcct tccatcttcg gcagcgtgat
     1141 cgcccgcgga tgctcgcggg tcgggtacat cttcgtcgag ggatgcagca cctgctcggt
     1201 cgactccggc gggttcttgt acaggtcgtc gacgcccttc cagccgccgt gctcgtacgc
     1261 ggccatgcac agcacggcgc ccttcatgta cgagtcgatg agtgggccga tgatcgcgag
     1321 tggcagctcg ccgatgctct ccatcgactt gcgcatgtcg tcgtccatct ggccgaacgc
     1381 attcgcttgc tgcttcatct gatcggcaaa cgccgagatg tccatcgccg ccatcgcctc
     1441 gacctgcgtc ttgacgaggt tcatcagcag attcgtcgtc tctttcgacg cgctgccgcc
     1501 cgacgcgctc gtgtagacga gcatcgcaaa cgtcgcgtca ccctcgacga cgaagtgccg
     1561 cgcgttcgtc tgatcgtcgt cgagacctga cggcaggtac ttcgcgagat cgaagtgctg
     1621 atcctgcagc gcgtgcgtca gctcgtgcgc ggacatcgtg tcgagcagca tctcgttgtc
     1681 gggcgccatc accacgaaga acttcttctg cgccgggtcg tagtaggcgg cggcctgcgt
     1741 gaccatcgtc ttgattagcg tgtcggcgag gtcgactggc ttgtcgagga ggccgatgtg
     1801 gagcagcgcg tcgcccatgt ccttcgactt cttcggcggc agctctttgt cgatgtcgcg
     1861 cttcaagaag gcgcggaagt cgtcggtcgt ctgatacgcc gccggcactt ccttcttgag
     1921 cggcagctcg cggagctttg ctagcttcgg ctcgatcgac tcgacgcgac tgaccgcggc
     1981 ctcagccgct ttgcgcttcg actccggcgt caccggtgct gcgtcgaccg tgacccacgg
     2041 atctttcgcc gacccgctgc ccgtcgcgac gacttcgatc gagccggagc ccgatccagt
     2101 cgcaggcttc gcgggcttgt cggtgttctt cgactggcac gcacaaagga cggccgcgag
     2161 cgttagccac ttcgacatgc cgaagtccta tcacgaggac ttcagaacga cccgtgtcgc
     2221 gccccagttc gcgtcagctt gctcgaagct cgccacggca ctgtggcgct cgagcacacc
     2281 ctgaacgatg cgacggagcg tccccgtccc cttgccgtgg atgatgcgga ccgtggtgaa
     2341 gcccgcttcc tgcgccgcgg tcagatactc gtcgacgacg tcggcgcact cgcgcggctg
     2401 gaacgtgtgg aggtcgagct ggtcggtcag ctcgggggtg aagacctcct cgtcgtccat
     2461 gcggccatga tacgaaggcg cgtgcggttc gtcgcggtgg ttgtggccgt agtcgcgtgc
     2521 tcgcggccgt tgtcgacgaa cacgtcgacg cacgcacact tgttgctcgt caacgggcac
     2581 atcttcacgg gcgatccggc gcaaccatgg acggacacga tcgcgatcgg cggcgacgag
     2641 atcgttgcga tcggcgacga ggcgaagcgc ttcacggcga accgccgcat cgatctgggc
     2701 ggccggctcg tcattcccgg catcaatgac gctcatgtcc acgagccgaa tctcttgccg
     2761 ttcgtcgacg tcgccgaagg cttcgacaag ctcaccgtcg ccgatctgct cgctgccttc
     2821 gagaaggccg agcacgagca accggcggga acgtggcttc gcggcgatct cagccaagcg
     2881 gcgctcgacg atccgacgct gacgaaggcg acgctcgaca gagtcgcgcc gacgcatccc
     2941 gtgtggctcg acaacttcgc cggtcacgtc gtcgtcatga acagcgcggc ggagaagctg
     3001 ctcggcctcg atcacaacgc gccgcacgcg ggcttcctcg gccgcgaaga cagcggggcc
     3061 gacgacgggt ggcgctacga atacgcgcgc tacgaagcga agcgccggat cggcctggag
     3121 ctctccgacg ccgatatcgc gcgcgcgatg cacgacttcg aacagcaagc gacgacgttc
     3181 ggcatcacga cggtccagac attcccgctc gacatcgaag cggagcggct cgtcatcgcc
     3241 ggcaatgtga cgcgccatgc cgtgcgctgg cacgtcatcc gcttcccgct cggctccgtc
     3301 atcgatccac cgcgcgcgtc gccgccggat gcctcgagcc ggctgtacct cagcggcacg
     3361 aagtacatcc tcgacggtac gccggtagag cgcggcgctg cactgctcgg cccgtatcac
     3421 gatcgcgcgg gctgggaagg tcgcgtcgat tggtcgcgcg aagaagtccg ccacatgctc
     3481 gtcgccgcgc gcgcaagcgg cgatgcgctg cacgtccacg tcgccggcga tcgctccgtc
     3541 gtgctcgtcc tcgatctcat gcaggagctc ggcggagatt ggcgcgcgag ccgcgtcgtc
     3601 ctcgagcacg gtgatgggct cgcggccgcc gatttcgcgc gtgcgcagca gctcggcgtg
     3661 atcgtcgtgc agaacccgat ccactcggcc accgcggagc tcaatcatgc gcggctcggt
     3721 gatcgcgcgc tgcattggat gccgatgcgc tcgctgctcg ccgctcgcat tccgttcgcg
     3781 ctcggttccg acggcccgct caacccattt ctcaacatca tgatggcgga gatgcatccg
     3841 acgaatcacg gtgaagcgct cacccgtgaa caagccgtca tcgcgtacac gcagggcgct
     3901 gcgttcgacg aacggctcga gcaacgcaag ggaaagctcg tgccgggcat ggtcgcggac
     3961 atcgccgtcc tgtcgcagga catcttcaag ctgcccgcag acgcgctccc cgccacgcgc
     4021 agtgtgctga cgatcgtcgg tggcgaagtc gtctacgacg ctagccgggc cagtgccccg
     4081 acttcggcac gaacggaatg attcgcgacc gcagcgaggg cggcacgcgc gcatcgagga
     4141 tcgacgcgac ctcgtcgggc cgatacagct gcgacagatg catcatcacg atgtgctcgt
     4201 tctcgaactt ctcggcccgc tcgatcacct cgtcgaggtg aatgtggcag cctgcgcgcg
     4261 cggcctcgag cgtcttgcgc gaatcgagga acgtgcattc catgatcagc acgcgcgtct
     4321 tcaggagctc gggcgaatgg tcgagcaccg acacgagcgt atcggtcgcg tacgcgagct
     4381 cgaggcgttc gtcgacgtcg agcatcggct cgccggcctg acgccgcgcg gcgatttctg
     4441 ggccggggag acctcggaat tcctcgcgca gcttcgtcac gcggcggacg atgatgtacg
     4501 cgagcgaggg cacggggtga aatgtccgga ccgcgcgcac gcgcaagtcg ccgcgcagct
     4561 cgatctcgtc gccgggcagc atcccgatcg cttcgacctc gagtggccat cgctgcagct
     4621 tgctcagcgc gccgagtgcg acgcgcagat cgtcgacgat ctcggccggc atcacgacgc
     4681 ggggcggctt ctgcttgccg tgaagtgccc gaattccgag cagggcaggg agcgcaccga
     4741 cgtggtcggc gtgcccatgt gacagcaaga tggtgtcgat gccgccggct gaccgggggg
     4801 caatgcctgc gtcgaagacg aggcccaatt cgggtaccgc cagcgaggtg taaacacctc
     4861 cgacagacac accgcggatg gtgaacggcc cggccgcgac ggtcgtcagc acgcgagcag
     4921 tgtagcgccc ctagatgcta ggatgttcgc cgcatgctct acgcgctgct agccgttgtg
     4981 gcggtcttgc tcctgctgtc gctggcactg ccggttccaa ggcgcatctg ggcgttcagc
     5041 ctgtacacga cgaagtactt cttcctctgg ctgtctgacg tcctcatgct gcgccgcctg
     5101 tggtttttga tcaccggccg cggcaagaag tatcagaagc tgacccgccc gatgatcctg
     5161 cgcatgttct gcgaggacct cggcccgacg ttcatcaagt tcggccagat catcgcgtcg
     5221 agcgcgggca tgttccccga tgcgtacgtc aaagaattcc agcgcgtgct cgaccgcgtg
     5281 aagccgttcc cattcgaaga cgtcaagcgc acgctcgtcg aggatctcgg cgccgagaag
     5341 gccgcgcacc tcgtcgacat cgagcccaag cagctcgcga gcgcatcgat cgcacaggtc
     5401 cacaccgcaa agctgcgtga cggcacgccg gtcgtcatca aggtccagcg ccccggcatc
     5461 atcgcgcgct gcatggccga catgaagatc atgcggttca tggccaagat cgtcgcgaag
     5521 ctcaggaaga acgtagagct cgcaaatccg gtcggcgtcg tcgacgactt cacgaagacg
     5581 ctcagcgagg agctcgactt ccgcaaggag gccgcgaacc tcgatcagtt caacatgatc
     5641 atgaaggagc tgggctacaa gcacatccac gctccggtcc cgcaccacga gtatacgacg
     5701 cgcaaggtcc tcgtgatgac gaagttcgtc ggcctgcgcg tcgacaagtt cgacgagatc
     5761 aaagcgcgcg gcatcgacgg cgaaacgaag ctcgtcgacg gtcttcgcgc gtggttccag
     5821 tgcgtcgtct tctacggctt cttccacggc gacgtccatg ccggcaacct catgctgctc
     5881 gacgacgaca cgatcggctt cctcgacttc ggcatcgtcg gccgcttcaa cgacgaagag
     5941 cgctacctcg tcaccgacta catgatcgcg ttcgcgtccg gtaactacaa acgcctcgcc
     6001 gagatcatca tcgagatggc gggtgcgccg gatggcctcg acatggaggc gttcgtcaaa
     6061 gacctcggcg atacgtaccg gccgctgctc acgctgtcgt tcggcgaggt gaactacgcc
     6121 gagttcctgc ccggcatcca gcgcacggcg acccgccaca agatgcgcat gccgaacgag
     6181 ttcatcctga tcacgaagca gctgctctac ttcgatcgct acgcgaagtc gctcgcgccg
     6241 aagctcaacg tcttcaccga tccacgcctc gtgctcggca tgatgagcga catccagaag
     6301 gcgcgcatgc aggccgacgc ggcgaagaag gcgaagtcgt cggcgaccac ttagtcgtcg
     6361 gcgattccgt acttcttcat cagccgcttc agcacgtagc ggttcgccat gccgagctcg
     6421 cgccatacct tctcgcggac accgtcgtgc ttcgcgagcg cggcttgaat ttgctcggcg
     6481 ctgtacggct gcgcggcgag ccgcggtttg tgtgcacgca ccagctcgct cgcgagtgac
     6541 tctgggagct cgaccgacga gccacggctc gcgacgagac ttcgcagcag gtaggagtcg
     6601 agctcgcgca cgttcgtcgt gtacttgtgg ccgacgagtg ctcgaacgag cgccggggtc
     6661 agccgcggtt cgccggtcgt accgtcccat ccagcgagaa agcgcgcacc gatcggcgcg
     6721 tccttgcgcg cgtggcgacg cagcaagtgg cgcgcgatca gcgggatgtc ctcgcgtcgt
     6781 gcgttgagcg acggcagggt gatgcgcagc gcgagccgag cagcgagatc atgcttgagc
     6841 gcatcggcgc cgcgattcgt cgctgcgact acgcgcagat ccgcggttcg tcgctttgcc
     6901 tcgccgagcc ggctgtattc gcctgcatcg agcacgcgca gcaggtggct ctgcagctcc
     6961 tgcggcatct cggcaatttc gtcgaggaag agcgtcgacc catccgcttc gccgatcacg
     7021 ccggggcgct cgggcatgcc cgcgttcgga tagctcgcga gattgccgaa cagctcggcg
     7081 tcgatgagcc ctggtggaaa cgtcgctgcg ttgcgtgcga cgagcttctt cgcgctgcgc
     7141 gtcgagtgcg cgtgaatcgc ctgcgcggcg agctccttgc cagtcccgct ctctccgagc
     7201 aacagcacgt gagcgctcag tgctccgacg cgcgcgcatt gttcgcggag ctcccacgcg
     7261 agcggcgatt cgccgacgat gccgtgcgca tcgggctcgc cgaacgtccc tgccttcgcg
     7321 tgcgactgcg gaagcgtccg tggccgatcg acgcacagaa acatcagctg gccgggaatc
     7381 tcgacggtct cgccaattcg cacgacgagc tcgctgtcgg ccgcctcgcc atcgagcagc
     7441 aacgccttct tgccgaagtt ctcgatcgcg atgccaccgg actcggctgc gatcgcgaga
     7501 tgccgccgcg agagaaacgg gttgtcgatc ggcaccgtcg cttcgagtcc acccggacgc
     7561 tggcgaacga gctcggctct cggctcgtca ccttcgcgcg gcccacgacc aaacacggcc
     7621 ggctcgccgt cggctctcgg tacgatcacc tcgccgacac gcgctggatc cgtggcagac
     7681 cacacgatca ccagcgccgc gacggtcggc tcgctgtcac gaaccgtgac ctcactggcc
     7741 gtcttgctgt cgagtgtcgt cgagctgctc acggtcctgg gtttcctgcc aggcacccag
     7801 cttaacatcg ccgtgatctc gccggtgccc gagctagtgc gacgtcgcag gcttcttgtc
     7861 gagccgcgcg atgagctggc gcatctcggc gacagcgggc acgtccacgt cgacgacgga
     7921 ccacgcgtcg atgaccttct ttgccatcgc gcgcgcctgt gcggcgtcac cgcgcttctc
     7981 ggcacggcgt gcttcgcgca cgtgagccat ctcggcgccg ttgaagcgac cgtcgcgcag
     8041 gtaacgcgcg tcgacgcgtg cggcgttctc cggatcgttg ccgcgctcga atgcgtcggc
     8101 gatggtgtcg tgcatgaaga acaggtgcca atccgggcgc gcgagcagcg ctcgccacgc
     8161 cttcgctgcg ccagcgaaat caccttgcga gtagcgctcc gcgccgtcga ggaaattcac
     8221 gtcgaagaag acgacgccgc ccgtcatctc ccgcaggcgc tgaaagcagt gcttcgcgat
     8281 gtcacgctgc gctttcatgc agaccagtgc gacgttgatc tcgatcagca cattgccttt
     8341 gacctgctcg ggatgtttgt cgacaaacct ctcgatgagc tcctgcgtac ctcgcgctgg
     8401 cttgccggtg acgaccgcga ggtcagacat gtaatacgcg gcattcaccg cgacttcggg
     8461 atccttgtcc ttcatctgca tctcggcccg ctccgtcgct tgcgcaaaat gtccctccgc
     8521 tgcatcgacc accaagttca ccgggtcggt aggcagtttg gccgctagcg atcgcgcttc
     8581 gtccaccttg ttgccgtgga cgaggaactc cacgagcgct tccttccaga tcgagccatg
     8641 cgagaggacg gtgttgcggc gcatgtacgc gatgccatcg tcggtcttgg ctgcgcgtgt
     8701 cgcggcgaag cagtagacct ccacattcca gggcgtccac gcgagtgacg ccggtgccgc
     8761 attgctgttc gcgcgcgtcc atgccacggc gttccagccg gtcgtccacg gaaagaatgc
     8821 gtcggcgctc tcgccgatgg attcgagcgc cgcttgggta gcctccggat acatgcgatg
     8881 gccggtaaag atttccgact tcgctgcaag cagaaacgcg cgcgcctgcc ggtctttcgt
     8941 gatggccaac gccttctcga tgcgagctag ctcggcgtcg agcggccgct tgctgagctg
     9001 tccatcggcc gtgatgctgg cgaccagtgt tccggggagc tcggtatcgc ggtccgggta
     9061 gtcctgcggc tcggggatgc accagttgtg gatcgccttc tgcgtgatcg gcaggatgtc
     9121 aggattcttc gacagcgcct cgcagccgct cttgttcggc cactcgagcc gattcccgat
     9181 cgaccactct gcaatcgcaa ccgcagcatc tgccgtgcgc gcgcccatcc actcgcgcag
     9241 gaatggatcg tcgtcgcgcg ccgcgggaac gaagcgatcg atcggtgcca tcgcatcgtg
     9301 gatcgcggga acgagcgtcg cgctgcgtcc gctgccatgc gcgagctcgc gatctccgac
     9361 tcgcagcacg agctcgacgg tgaagccatc cgcggtgtgg tcgatcgatc catcgagcca
     9421 cgcctggcct cgcgtcttcg ccgccgcgat cgcgcgttca cgtgcgtcgg ctgcgctaaa
     9481 cgcatcctcg gggaagttgt cgacgacggt gcgcggcaga tcgagcagct ccgcagggag
     9541 cagcgtacgc tctgcgcgat tgcccatgcg cacggtcgcg cgcgtgcatg cgatcgatgc
     9601 ggcggcggca ccgagccatc ccgcgggctg ctcgacacca cttgcgacga gcggcgggca
     9661 cgccagaacc gatcccgccg ccttcagcgg gctcgttgcc attcgacccg ccgcgccgcc
     9721 cgctgcccca ttgcccgcat cacccttcgc gctcgcagtc ctgcgctgcg aatcggcgcg
     9781 cacacatacg agcgtgctcg tcgcgagcgc agcgaggccc gcaaacgcga agccgctcca
     9841 cgcgagacgt cgacggcgcg cctgcgtgcg ctcaacgccc atcgagattc cggcgcgcgc
     9901 ttcgatttga ccgcgcgcat cgctgtgacc gcgcgcttcg atctgcccgc ggccttcgct
     9961 cgtcgatgta ccgacaccgc tcgcattcgc gcttgcgccc gccgccacgg cgttcgcggc
    10021 agccaccgag ttcgcggtag cgagaggtgc atcgatgttg gcgatggccg gcccgctgat
    10081 tttcgaggcg agtcgggcgg actcgatctc ttctcgactt gcggacgcag tctcggcgag
    10141 acccgcaagc tttcgttcgt ccgatgcgtc gaccgcgaac ggcgcgagta cgcgcgccag
    10201 cgacgcgaca tcggcaaacc ggtcggccgg cttcttcgcg aggcagcgcg ccacgatcgc
    10261 atcgagatcg accggcacgt ccggcctgat cgacttcacg gatggcgccg gctcgtcggc
    10321 gatcttcttg tagagctgcg gcagcgtgtc ggccacgaac ggatgcgcgc cggtcaagag
    10381 ctcgaacaag atcacgccga ggccccagat gtccgtgcgc gcatcgatct cgcgcggcga
    10441 ctcgagctgc tcgggcgaca tatacgccgg cgagccgagc accgacgccg tgctcgtcag
    10501 cgaaggactc actcggtgcg aatccgtgct cacgaacttc gagattccga agtccaagac
    10561 cttgatcgcc gacttgtcgt cgcggactgc gaggaacatg ttcgccggct tgaggtcgcg
    10621 atggatgatc ccgatcgcgt gcgccttccc gactgcatcg catgcctggc gcacgtacga
    10681 gcacgcgtcc gcgacatcga gcttgccctc ggcgcacaac agctgcgcga ggtcgcagcc
    10741 ggccaagtac tccatcacga tgtacggcga gccgttctcg agcgtgccga cgtcgaacac
    10801 gcggacgacg tgatcgctct ggatcttcac cgtcgcacgc gcttcgcgca gcgccctctc
    10861 caccgcatcc gcttgcgcga ggcgatccgc gcgcaacagc ttgatcgcga cgcgctcgtc
    10921 gagctgttcg tgcagtgcgg ccactacgac gcccatgcca ccggttccga gcacgcgctc
    10981 cacgcggtac ttgcccgcca gaaccgttcc cgggttcgtc tccaccatcg tctgcgagag
    11041 ggtacgtgtc attgtgccgc cctctcaagc aacgactgca ccacgtagcg gacgaacgat
    11101 tctcgacgct tgcgcgctcg cccgcgtgcc cgcccagagc ccgcccatgg gcgcgcccat
    11161 tccgggccta gccgaagcgc ttgtcgacgt cgaggctcga tgagcgcggc tgcttcgccg
    11221 ctcgctcgtc acccaggtac ttgagaaaca gctgcagata cttcgagcgc tcggtatcga
    11281 gcggcttgaa cgagacgccg acctgaaacg cgttgtcgac ggtcgtgcac cacgcgacgc
    11341 gtgcaggcag tcgcagagct tccgactgca tctcgtcgtc gaacacgagc acgatgtcga
    11401 cctcgacgtc gctgcccgct ttgatctcgg cttcgacgtc ggcgcagagg ccaccgcgcg
    11461 agacgttcgt cgtgcgtccc gccgtcgtac gcttaccgaa gtggatggtg acagccactg
    11521 cgtgcgcgta acgcggatgt tgccgctcga ctgatccgct cacttcccga aattgtgact
    11581 ggcctacacc gacgctggca agtttccgct ggggtagccg tccggtgtcc gaagaccgga
    11641 cagcaattca gggtagttcg gaggacattc tgtgatgcgc gctatcggga ttcccgacgg
    11701 ttcgcgccgc ggttctccga aaaccacccg tccgtgaggg cgtccagctc cgccgcagcc
    11761 tcggcaaccc gcagctccca ccgtccgtcg cgttcgacgg caacggacgc gcggagccac
    11821 acgaagtacg cacgactggt gactgcggcc aggagccgcc gctcgcgccg gcgagacagc
    11881 tcgaacgcgg taaagcgcgg ctcgttggga tcgtagagga ggtgatcgag gtgccacgcg
    11941 acgcgcgcgt catacgataa cgagtggttg agggtcggcg cgctcgacga cgagcttgcc
    12001 gcaaccgctg gatcgagaat gtcgcgccag ctctggctgt tgccgactcg cagcgagatg
    12061 actgggatga gcgcagacca ccgcgaccgt cgtcgccaac tcgccgtcgg cgcgtctgcc
    12121 cccgcgagcc cagcgatcga ctgcgcgcgt gcgatgatcg tagagagctc gggcgcggtc
    12181 gccgcgattc gcgacagcgt cggtgcatcg cagtcgcaca gcgcgtcgat gccagctctt
    12241 cgcccgcggt ccgcacccgc cgacccgccg actgcatacg gaagatcgac actgacgtca
    12301 tcgtcaccgt catcgccgtc gatggcgtcg tcgaatccac ggtcggttcc gtcggggatt
    12361 gcgtcgtgga gtccgcccgc gcctccgcgc tggcttccgc gctcgattcc gcgccgatct
    12421 gcgggcgacg catgggcgcg cgcggcgagg gccacgagga gcccgacgac tgccacgacg
    12481 atgaggccag cgacgtgacg acgctctgtc aggtttcctc ggccatcccg tcgacgccgg
    12541 ttgcgtgatg gggccccggc ttgtagggtc cgtcggtgac cgcgacgctg accgacaacg
    12601 ctctccaggt gctgcgcgcg cgctacctgg ctcgcgagaa cggcatcgtc gtggagacgc
    12661 cggatcagct cttccgcagg gtcgcccgtc atatcgccgc ggtcgagtcc gggctcggct
    12721 acagcgaggc tgaggtcgag ggagcgcgcg cgaagttcga gcggatgatg tcgtcgctcg
    12781 agttcgtgcc gaacagcccc actctcatga acgcaggccg accactcggc cagctcgcgg
    12841 catgcttcgt cgtgccggtc gatgactcca ccaccggtgt cttcgaagcc gtccgctggg
    12901 ccgctgaaat ccagaagaca ggcggtggca ccggcttctc gttctcgcgc ttgcggccag
    12961 cgggcgatct cgtcgcctcg accggcggca acgcgtccgg ccccgtctcg ctgatgcagg
    13021 tcttcgacgt cgcgaccgaa gcgatcaagc aaggcggcac gcgacgcggc gccaacatgg
    13081 gcatgttgcg cgtcgatcat cccgacgtcc tcgagttcat cgcgctcaag ctcgatccaa
    13141 ctcgcatgcg caacttcaac ctgagcgtcg cgatcaccga cgcgttcatg gctgcggttg
    13201 ctgccggcac gacgtacgac ctcatcaacc cgcgcacgaa ggtcgtcacc ggccagctcg
    13261 acgctcgacg cgttttcgat gcgatcgcga atgcggcctg ggccgtcggc gatccggggc
    13321 tcgtgttcat cgatcgggta aacgagctcc acccgactcc agcgctcggt gcgatcgagg
    13381 cgaccaatcc atgcgtcgtc gggggcacgc tcgtgtggac cgaagaacga ggcctcgtcg
    13441 cgatcgaaca gctcgtcggc gaacggccgc gggtagcgac actcgacggt gagcgcgtcg
    13501 tgttccgcga agccagccag gtcatcgcta ccggcgtgaa gccggtcttc cgactgatca
    13561 acgatcgcgg gctccacctc atctgcactg cggaccaccg aatcacgacg agccgcggtg
    13621 atgtagccgc gcaggatctc gtcgtgggcg acaccattcg cgttgtcgcg ggcggtcgca
    13681 gcgacctcgc gtcgggcgct cgactcgacc tcgccgccgg ctcgctccag acgcatctcg
    13741 gctgggcgac gtttcgcggc ctcgagccgc tcggcgacgc gcccgtcttc gatctcaccg
    13801 agccgaccac cagccacttc tttgcggatg gcgttctcgt tcataactgt ggcgagcagc
    13861 cgctgttgcc gttcgagtcg tgcacgctcg gctccatcga tctcgggcgc ttcgtacatg
    13921 gcggcgactt cgactgggag cgcctccggg aatgcatcca cgacgccgtc cgctttctcg
    13981 acgacgtgat cgacgcgaat cggtacccac tcctcgagat cgagcgcgcg acgaaagcca
    14041 ctcgcaagat cggtctcggc ttcatgggct gggccgacgc actcgtgctg ctcgacatcg
    14101 cctacgacag cgacgctgcg atcgagctcg ccgatcgcgt ggctgcgttt ctcgagaccg
    14161 agtcgcttgc cgcttcggct gcgcttgccg accggcgtgg cgcgtttccg tcgtgggacc
    14221 ggtcgcgttg gcagaccgct ggccaccggc ccttgcgcaa tgccaccacg acgacgatcg
    14281 cgccgaccgg cacgatcagc atcatcgctg gatgcgcgag cggtatcgag ccgctctacg
    14341 cgctcgcgta tcggcggaac gtgctcgacg gcgccgagct gaccgagatc aatcccgcgt
    14401 tccaacgcat cgcgagcgag cgcgggttcg cgtcgccgga gctgttcacg accgtcgccg
    14461 agcacggcgg agtccgcggc aacgacgccg tcccgaccga cgtgcaacgc cggttcccga
    14521 ccgcgcacga cgtctcggtc gacatgcacg ttcgcatgca ggccgccttc cagcgccacg
    14581 tccacgctgc cgtcagcaag acgatcaacc ttcggcgcga agccagtgcg gctgatgtca
    14641 aagctgcgta ccagctcgcg tacgagcttg gttgcaaggg aatcaccgtg taccgggacg
    14701 gcagccgcga gggccaagta ctggtcaccg gtgcacgcgg gccggtggtg agtgccacgc
    14761 cgagctgccc ggagtgcggc tcggtgcttg tggtccaaac gcattgccgt ctctgccggc
    14821 attgcggctg gtccgtctgc ggctgacgcg ctcctgccag cttcgtcgtt ggtcgcggta
    14881 gcttcgtgat cggtttgcat cgtgcggcgg cgatgagcaa gacctcggcc attcgctcgc
    14941 tcgtgcgatc acgcatcgtt acgccgcgcc gagcgcgcga ttcgtccgag caccggacaa
    15001 agcgtccggc aggcgttcgg acaccggaca atcgccttcg aatcgccagc tcgtcatgac
    15061 ggcgtcatga cgggtcccga aaagagac
//
LOCUS       DATLBZ010000397         8966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5684048, whole genome shotgun sequence.
ACCESSION   DATLBZ010000397 DATLBZ010000000
VERSION     DATLBZ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5684048"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VMZ53_20105"
     CDS             <1..1103
                     /locus_tag="VMZ53_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30830.1"
                     /translation="QALGDFPFVIGSILNVKEVYYGTIKTANYLDKTKRSWRGDGSYA
                     DEDVKFTMTIPKSTSNGPMPVVIFGHGLVTERRFVLAIGDALAAKGYAAISIDFPYHG
                     SRTYCAKGGPVSIVDPLDGSLASLEPCSSGYTCNDEGRCVDSQGNGNHLATFGVIDLP
                     VASGAMFLEIDHIANSKDHFQQALIDLGALDRSLRLGNWQSVVGRPIDTSKIYYAGQS
                     LGGIMGALFLGTDPDIKRAVLNVPGANLVPMFDESTFFSAQLDAFFTRQKVQRESFEG
                     RRFMTVAKWFMDAVDPEHVGPVTGNRALMIQMATLDFIIPNDNTKTLQSVTKAPRRDY
                     VAEHGFLVIPLEPEYWRGTRDLANFLSGELVP"
     gene            1100..2296
                     /locus_tag="VMZ53_20110"
     CDS             1100..2296
                     /locus_tag="VMZ53_20110"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HUS30831.1"
                     /translation="MKKVLLAILVSSSAAHAGGTEVAEQNAVSAGTGGAGAARDNDAG
                     AAWHIPAALADDGGTRIGISLALAHPSLQAEGSTGWSAESENAWATPPHIDASYAHGR
                     FAAGVALGVPFGGGVTWPSTWERSSEAINTQLIVLRAAPFAAYAVADGKLRIAAGLHF
                     DAGRLQVERGLDFIDTQGTVKLDLDGRGTGVDASAFYAARPDLGIGLAFRSRTHITFD
                     GNANFTTPDAFSEKTPDQTAQTHMTLPDQFVLGARWHRDTLSILGDITYTNWSVNQRT
                     DVDFQMDQTPTATQMNEWHDTVGLRAGAELQSTRSLIARFGAYFDPSPVPAEHLTPTA
                     PDATRVALTAGASYRINAAWSADAFAEQMWLLRRDTTSVDTMPASYGGTAVVLGAGVR
                     WTPSAE"
     gene            complement(2330..3898)
                     /locus_tag="VMZ53_20115"
     CDS             complement(2330..3898)
                     /locus_tag="VMZ53_20115"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30832.1"
                     /translation="MASFWRRLFGRQTQPPAIAPPVAPEPQHLSSEEEVFLAQLVQDL
                     ADGKRRDEIGTPTVLAKLDGLWKAGHERLAIEWTEKLLTVPEVPAEASAPLRAAIVER
                     YDQRGELDTAVRHLDHLVNLEPYALRAHYLLAEHARRKNDHERALRHYEAVLGRDVDY
                     PNVRVRVERLRQLTGRAAPVAGETIAAGEVAGIQAGARYRLMRELGRGATGVVYLARD
                     AELDRDVAVKLLHPHLAGNDRAESLARFFHEARVMASLRHPNVVAVLDMDEASRRIVM
                     ELAAGGTLRDVLRERGPRTLRRALERHGQILSALAAAHRRGIVHCDLKPANLMFRRDT
                     DLPGVEIMLGDFGVAHLPNELGQAVVEGAAPAIASSGASAPIVGGASGPIAGGTSGPV
                     PKPDASGATRRDGSGPSKKDAVGTLAYMAPEQRRGEISPAADVYAAAVVLFEMLTART
                     PGSREGVIGGRRGASDFKLPAELFAAAPSVQLDVQDHLDRISHIDPAIRPTTAQALAE
                     SQRLRERVIAAGAI"
     gene            complement(3937..5115)
                     /locus_tag="VMZ53_20120"
     CDS             complement(3937..5115)
                     /locus_tag="VMZ53_20120"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS30833.1"
                     /translation="MSFFRRFSADHRAAVAAEAAGNVDLAAERYALAGDHEGAVRMHL
                     ARAARAATRNAEIGALRDALRWAGEDPQLQKQASKALGRALLENARAEGIATERDRTK
                     VREAAEMLVAGDDHYSAGEALESIGDHQAAALAYSAGGLVEKMEQALSKEDQRYLKAR
                     EEADAFADYETHMRTGRRDDARAELARALAATDKVGEYRRLLDQLDTALLTAGKVELK
                     RRSKPAIIACAASKIVLGRDALCDVALRAGGVSRQHAEIERAIDGFHLRDLDSRNGTS
                     VAGLPLVGKVPLAGAGKFGLGNECSIEFEAKDGVLIARVANGLDRGLAIIAGDDGQRL
                     ELTGLGLGLDVMFQKGRPTLGRGSCKEVRFNDEPLGELRVQLIRGDRIVADGDEIDIG
                     "
     gene            complement(5274..7160)
                     /locus_tag="VMZ53_20125"
     CDS             complement(5274..7160)
                     /locus_tag="VMZ53_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30834.1"
                     /translation="MRWALVCFLIAAACGGQQIQTRNAADLSKLGPATLEASQPKEGE
                     PRTAKVRIWVDAGVRAMPGYKDKISEQVDYAGQLLTPLLGVQLKIESFKDWDREAPDA
                     ANPAGDPAEALTQLKTVDPGKDVAWVIGYMMPNSVASTAMIALGSGELLGHHVVIRAW
                     AEKQEADVLSDKLVDLKPAERGEVLAAHERHKQTVVLVHWLAKTLGAVDEADEAWIGN
                     PAYSPKQSSFADRTRDILQLGIDARVSETADKDIAHDLLEKLDKENWGGWVASSKEEI
                     TQRLRAIVTAAKQGEAAPDVPVAAVEQFERTKAMTGASLNKLGAAKKPDEKKAAEQSF
                     DTAMAELDNLLTAYPGNAAMYTFKCEALLARRGPTDKTARAACVRAAELAVSDPRPNF
                     VVANSLLASKDIAGARKELQTAATKIGQLKANQADAWKALIAIYQSIGSLTWTEEAIA
                     QAKLEKDDITPQIDSTRARYGVPKGAKFVKPEQEGELVTTVKTALSLVYANKFADAEK
                     TIAAGEKKWPGAAGLAAVRCDLALRQTKLEPARAACERALATDPNESWALYLSGVIAL
                     KDTSNAGTARGIEQLKRAIAVDPDLGQAWRTLAKAYSRAKDQAALDKRAGEYQQKFGS
                     PLPR"
     gene            7213..7644
                     /locus_tag="VMZ53_20130"
     CDS             7213..7644
                     /locus_tag="VMZ53_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30835.1"
                     /translation="MTDRLLLDERILGDGHLARTYASFVEGQLHIDDQASGPDSAHGA
                     LSIAAVDRVMVRYGLPLEQGLRVEGDALDLGDGRALRRYRFHAKVDAEARDYLVWERP
                     GEEPIAVLATHATAALRYLVLRLGEERRDSAQNKAPQESEG"
     gene            7742..8899
                     /locus_tag="VMZ53_20135"
     CDS             7742..8899
                     /locus_tag="VMZ53_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30836.1"
                     /translation="MLRCLWLSCILSVAPVTAWAQSAQPAPTAVVDKAAQSTTAAEKK
                     VATLANQRAQLTATLAQQVATADALKKQKASWRRDRELKTALAAANDTAAKLTTLDKQ
                     LATAQLALATARKTELVAIDAELKAGPTPARVDQLNKLRAHLTPAPIATKKIVIPDAE
                     IDPLADPDDLEKQAAAIAAAEKALERERTSLDKQHGDLVAMANLRSAHERAGELSTRD
                     DDQPHRNAPRGGRSAEVGAQNTGDDSAGSPAPGGGGSTGGNGGGSGAETGGGGGSTGG
                     GSTGGDLFDGSKTTTSFESSAAIALGEVVDQSTIDGLLRARNSGDPKQRADAAAKARD
                     AVQKRLELLKKKREMIEKRAKALRKGASTRTPRTDEMLFSELLAPPSTSVS"
BASE COUNT         1457 a         3190 c         2869 g         1450 t
ORIGIN      
        1 cgcaggcgct cggtgacttc ccgttcgtca tcggctcgat cctgaacgtc aaagaggtct
       61 actacggcac gatcaagacc gcgaactacc tcgacaagac gaagcgctcg tggcgcggcg
      121 acggcagcta cgccgacgaa gacgtgaagt tcacgatgac gattccgaag tcgacgtcga
      181 acgggccgat gccggtcgtc atcttcggcc acggcctcgt caccgagcgg cgcttcgtgc
      241 tcgcgatcgg cgacgcgctc gccgcgaagg gctacgccgc catctcgatc gactttccgt
      301 atcacggctc gcgtacgtac tgcgcgaagg gtggcccggt cagcatcgtc gatccgctcg
      361 acggcagcct cgcatcgctc gagccctgca gctccggcta cacgtgcaac gacgaaggcc
      421 gctgcgtcga cagtcaaggc aacggcaacc acctcgcgac ctttggcgtg atcgacctgc
      481 ccgtcgcatc cggcgcgatg ttcctcgaga tcgatcacat tgcgaacagc aaggatcact
      541 tccaacaagc gctgatcgat ctgggcgcgc tcgaccgctc gctgcgcctc ggcaactggc
      601 agtccgtcgt cggtcgcccg atcgacacct cgaagatcta ttacgccggc caatcgctcg
      661 gcggcatcat gggcgcgctc ttcctcggca ccgaccccga tatcaagcgc gctgtcctca
      721 acgtaccggg cgcgaacctc gtgccgatgt tcgacgagtc gacgttcttc agcgcgcagc
      781 tcgacgcgtt cttcacgcgc caaaaagtcc agcgcgagtc gttcgaaggc cggcgtttca
      841 tgaccgtcgc gaagtggttc atggatgcgg tcgatcccga gcatgtcggc ccggtcaccg
      901 gcaatcgcgc gctgatgatc cagatggcga cgctcgactt catcattccg aacgacaaca
      961 cgaagacgct gcagtccgtg accaaggcgc cgcgccgcga ctacgtcgcc gagcacgggt
     1021 tcctcgtcat cccactcgaa cccgagtact ggcgaggtac gcgcgacctt gccaacttcc
     1081 tcagcgggga gctggtgcca tgaagaaggt cctactcgca atcctggttt cgtcgtcggc
     1141 cgcacatgcg ggcggcaccg aagtcgccga acaaaatgcc gtctccgccg gcaccggcgg
     1201 tgcaggcgca gcgcgcgaca acgacgcagg tgcggcgtgg cacatcccag cggccctcgc
     1261 cgacgacggt ggcacgcgca tcggcatctc gctcgccctc gcccacccat cgctgcaagc
     1321 cgaaggctcg acgggctggt ccgcagagag cgaaaacgcg tgggccacgc cgccgcacat
     1381 cgatgcgagc tacgcgcacg gtcgcttcgc agcaggcgtc gcgctcggtg tcccgttcgg
     1441 cggtggcgtc acgtggcctt ccacgtggga gcgctcgagc gaagcgatca acacgcagct
     1501 gatcgtgctg cgcgccgcgc cgttcgccgc ctacgcggtc gccgacggca agcttcgcat
     1561 cgccgcaggt ctgcacttcg acgcgggtcg cctgcaagtc gagcgcggcc tcgacttcat
     1621 cgacacgcaa ggcaccgtca agctcgacct cgacggtcgc ggcacgggcg tcgacgccag
     1681 cgcgttctac gccgcacgcc ccgatctcgg catcggcctc gcgttccgca gccgcacgca
     1741 catcacgttc gacggcaacg cgaacttcac gacgccagac gcattctccg aaaagacgcc
     1801 cgatcaaacc gcgcaaacgc acatgacgct gcccgaccaa ttcgtcctcg gcgcgcgctg
     1861 gcaccgcgac acgctctcga tcctcggcga catcacgtac acgaactgga gcgtcaacca
     1921 acgcaccgac gtcgacttcc agatggacca aacgccgacg gcaacgcaga tgaacgaatg
     1981 gcacgacacg gtcggcctcc gcgcgggtgc cgagctgcag tcgacgcgct cgctcatcgc
     2041 ccgcttcggc gcctacttcg atccatcgcc cgtccccgcc gagcacctca cgccgaccgc
     2101 ccccgacgcc acgcgcgtcg cgctcaccgc gggtgcgagc tatcgcatca acgctgcgtg
     2161 gtccgcagac gcgttcgcgg agcaaatgtg gctgctccgc cgcgacacga cgagcgtcga
     2221 cacgatgccc gcgtcgtatg gcggtaccgc ggtcgtcctc ggcgcaggcg tccgttggac
     2281 gccgtctgcg gagtagcgcc cgagctgccg gagaaagccg acgcggtgct cagatcgcgc
     2341 ccgcggcgat cacgcgttcg cgcaggcgct gcgactcggc gagagcctgc gcggtcgtcg
     2401 gtcgtatcgc tggatcgata tgcgagatgc ggtcgaggtg atcctgcacg tcgagctgca
     2461 cgctcggtgc ggcggcgaac agctcggccg gcaacttgaa gtcgctcgcg ccacgacggc
     2521 caccgatcac gccttcgcgc gagccgggcg tacgggccgt cagcatctcg aagagcacga
     2581 ccgcggcggc gtacacatcg gcggctggcg aaatctcgcc gcggcgctgc tcgggcgcca
     2641 tgtacgcgag cgtgccgacg gcgtccttct tcgacggccc ggagccatca cgcctcgtcg
     2701 cgcctgacgc gtcgggcttc ggcacgggtc ccgacgtgcc accagcgatc ggccccgacg
     2761 caccgccgac gataggcgca gacgcgccgc tggaagcgat cgccggcgcg gctccttcga
     2821 cgaccgcctg gccgagctcg tttggcaggt gcgcgactcc gaagtcgccg agcatgatct
     2881 cgacgccggg cagatcggtg tcgcggcgga acatcaagtt ggcgggtttc agatcgcagt
     2941 ggacgattcc tcgtcggtga gcggctgcga gcgcgctgag gatctggccg tggcgctcga
     3001 gcgcgcgacg cagcgtgcga ggaccgcgtt cgcgcaggac gtcgcgcagc gtgccgcccg
     3061 cggcgagctc catcacgatg cggcgcgagg cctcgtccat gtcgagcact gcgacgacgt
     3121 ttgggtggcg gagcgatgcc atcacccgcg cttcgtggaa gaaacgcgcg agcgattcgg
     3181 cgcggtcgtt gccagcgagg tgcgggtgca gcagcttgac cgcgacgtcg cgatcgagct
     3241 cggcatcgcg cgcgaggtag acgacacccg ttgcaccacg accgagctcg cgcatcaggc
     3301 gatagcgcgc acctgcttga atgcctgcga cttcgcccgc agcgatcgtt tcgcccgcga
     3361 cgggagctgc acgtccagtg agctggcgca ggcgctcgac gcggacgcgc acgtttgggt
     3421 aatcgacgtc gcggccgagg actgcttcgt agtggcgaag cgcccgctcg tggtcgttct
     3481 ttctgcgagc gtgctcggcg agcaggtagt gcgcgcgcag agcgtagggc tcgaggttga
     3541 cgaggtgatc gagatgccgg accgccgtgt cgagctcccc gcgttggtca tagcgctcga
     3601 cgatcgcggc acgcagcggc gcgctcgctt ccgccggcac ctcgggaacg gtgagcagct
     3661 tttcggtcca ttcgatcgcg aggcgctcgt gaccggcttt ccacaggccg tcgagcttgg
     3721 cgagcaccgt tggtgtcccg atctcgtcgc gccgtttgcc gtcagcgagg tcttgaacga
     3781 gttgcgcgag gaagacttct tcttcgctac tgagatgttg cggctcgggc gcgaccggcg
     3841 gcgcgatcgc gggcggctgc gtttgccggc cgaacaacct gcgccagaag gaagccatcg
     3901 ctcgcctact ttacccgcgc cgcggtcgat gcgctcttag ccgatatcga tctcgtcgcc
     3961 gtcggcaacg atgcggtcac cacggatcag ctggacgcga agctcgccaa gcggctcgtc
     4021 gttgaagcgg acttctttgc agctgccgcg gccaagcgtc gggcggcctt tctgaaacat
     4081 gacatcgagg cccaggccga ggcccgtgag ctcgaggcgc tggccgtcgt cgccggcgat
     4141 aatcgcgagc ccgcgatcga ggccatttgc gacgcgcgcg atcaagacgc cgtcttttgc
     4201 ctcgaactcg atcgagcatt cgttgccgag gccgaacttg cccgcaccgg cgagcggcac
     4261 tttcccgacg agcggcagcc cggcgacgga cgtgccgttg cgggagtcga ggtcccgcag
     4321 atgaaacccg tcgatcgcgc gttcgatctc ggcgtgctgg cgcgagacgc cacccgcgcg
     4381 cagcgcgacg tcgcacagcg catcccgccc gagcacgatc ttcgacgccg cgcacgcgat
     4441 gatggccggt ttgctgcgac gcttgagctc gactttgccc gcggtcagca gcgcggtgtc
     4501 gagctgatcg aggagccgtc ggtactcgcc gaccttgtcc gtcgctgcga gcgcgcgtgc
     4561 gagctcggcg cgagcatcat cgcggcggcc agtgcgcata tgcgtctcgt agtcggcgaa
     4621 cgcgtcggct tcttcgcgcg ccttgaggta gcgctggtcc tctttcgaca gcgcctgctc
     4681 cattttctcg acgagaccac cggcggaata cgcgagcgcc gcagcctgat gatcgccgat
     4741 gctctcgagc gcttcgccgg cgctgtagtg atcgtcgccg gccaccagca tctcggcagc
     4801 ctcgcgaact ttcgtgcgat cgcgctccgt cgcgatgcct tcggcgcgag cattctcgag
     4861 gagcgcgcgg ccgagcgcct tcgatgcttg cttctgcagc tgtggatctt cgcctgccca
     4921 gcgcagtgca tcgcgcagcg cgccgatttc ggcattcctc gtcgccgcac gcgcggcacg
     4981 cgcgaggtgc atgcgaacag cgccctcgtg atcgcctgcg agcgcatagc gttccgccgc
     5041 gagatcgaca ttccctgccg cctcggccgc aaccgcggcg cgatgatctg cagagaaccg
     5101 gcggaagaaa ctcacggcac gagtatagag gaagcggctc gcgcggcacc ggcctgccga
     5161 acgtcaagac aatcaccggc agatggctat cgagcaggtt cgtggtcagc gaggcagcgg
     5221 cctgccgaac gccaagacaa tcaccggcag atggctatcg aggaggttcg tggtcagcgc
     5281 ggcagcgggc tgccgaactt ctgctgatac tcgccggccc gcttgtcgag ggcggcctgg
     5341 tctttcgcgc ggctgtaggc cttcgcgagc gtgcgccacg cctggcccag atccggatcg
     5401 acggcgatcg cgcgcttgag ctgctcgatc cctcgcgcgg tcccggcgtt gctcgtgtcc
     5461 ttgagcgcga tcacgcccga gagatagagg gcccacgatt cgttcggatc ggtcgcgagt
     5521 gccctctcgc acgcggcacg cgcgggctcg agcttcgtct gacgaagtgc caagtcgcag
     5581 cgaacggccg cgagccccgc cgcaccgggc cacttcttct cgcccgccgc gatcgtcttc
     5641 tcggcgtcgg caaacttgtt cgcgtagacg agcgacagcg cggtcttcac cgtcgtgacg
     5701 agctcgccct cttgctcggg cttcacgaac ttcgcgccct tcggcacgcc gtagcgcgca
     5761 cgcgtcgaat cgatctgcgg cgtgatgtca tccttctcga gcttcgcctg cgcgatcgct
     5821 tcttcggtcc acgtgagcga gccgatcgat tgatagatcg cgatgagcgc tttccacgcg
     5881 tcggcttggt tcgccttgag ctggccgatc ttcgtcgcag ccgtctgcag ctccttgcgc
     5941 gcgcccgcga tgtccttgct cgcaagcagc gaattcgcga cgacgaagtt cggccgcgga
     6001 tcactcaccg ccagctccgc tgcacgaacg cacgccgcgc gcgccgtctt gtccgtcggt
     6061 cctcgtcggg cgaggagcgc ttcgcacttg aacgtgtaca tcgccgcgtt gccgggatac
     6121 gcggtgagca gattgtcgag ctcggccatc gccgtatcga acgactgctc ggccgccttc
     6181 ttctcgtcgg gcttcttcgc ggcgccgagc ttgttgagac tcgcgccggt catcgccttc
     6241 gtgcgctcga actgctcgac cgcggcaacc ggcacgtcag gcgctgcttc gccctgcttc
     6301 gctgcggtga cgatcgcgcg cagtcgctgc gtgatctctt ctttgctcga cgccacccag
     6361 ccgccccagt tctccttgtc gagcttctcg agcaagtcgt gcgcgatgtc tttgtcggcg
     6421 gtctccgaga cgcgtgcgtc gatgccgagc tgcaagatgt cgcgcgtgcg atccgcaaag
     6481 ctcgactgct tcggcgagta cgcgggattg ccgatccacg cctcgtccgc ttcgtcgacc
     6541 gcgcctagcg tcttcgccag ccaatgcacg agcacgacgg tctgcttgtg gcgctcgtgt
     6601 gccgcgagca cttcgccgcg ctccgccggc ttcagatcga cgagcttgtc ggacagcaca
     6661 tccgcttcct gcttctctgc ccatgcgcga atgacgacgt gatggccgag cagctcgccg
     6721 cttccgagcg cgatcatggc cgtgctcgca acgctgttcg gcatcatgta gccgatgacc
     6781 cacgcgacgt ccttacccgg gtcgacggtc ttcagctgcg tcagcgcttc ggccggatcg
     6841 cccgcgggat tcgcggcatc gggcgcttcg cggtcccaat ccttgaacga ttcgatcttg
     6901 agctgcactc cgagcagcgg cgtgagcaac tggccggcgt aatcgacctg ctcgctgatc
     6961 ttgtccttgt agccgggcat cgcgcggacg ccggcatcga cccagatgcg aactttcgcc
     7021 gtgcgtggct caccctcttt gggctgcgac gcctcgagcg ttgcgggacc gagcttgctg
     7081 aggtcggcgg cgttccgcgt ttggatttgc tgcccgccac acgcagcagc gatcaggaaa
     7141 cagacgagcg cccagcgcat gcgtggactt tccccgttga ttggcgagcg cgcaagccgc
     7201 accatcgcgc gcgtgacgga tcggctgctt ctcgacgagc gaatcctggg tgatggccac
     7261 ctcgcgcgca cctacgccag cttcgtagag ggccagctcc acatcgacga ccaagccagc
     7321 ggtccggact ccgcccacgg cgcactctcg atcgccgccg tcgatcgcgt gatggttcgc
     7381 tacggcctgc cgctcgagca aggcctccgc gtcgagggcg acgcgctcga tctcggcgac
     7441 ggccgggccc tgcgccgcta ccgcttccac gcaaaggtcg acgcggaagc ccgcgactac
     7501 ctcgtctggg agcgccccgg cgaggagccg atcgccgtcc tcgcgaccca cgcgacggca
     7561 gcgctccgct atctagtcct tcgactaggc gaagagcgcc gagattcagc ccaaaataag
     7621 gcccctcagg aatctgaggg gtgacgcccg ccggccttca cgatctagcg gctagccgcc
     7681 atcagagacg cccgatccca agtacttgga cgtggcacgg tcactgctat gtaataggtc
     7741 cgtgctgcgg tgtctttggc tttcgtgcat cctctcggtg gctccggtca ccgcgtgggc
     7801 gcagtctgcc cagcccgctc cgaccgccgt cgtcgacaag gccgcgcagt ccacgaccgc
     7861 cgctgaaaag aaggtcgcga cgctcgccaa ccagcgcgcg cagctgaccg ccacgctcgc
     7921 gcagcaagtc gcgaccgctg acgcgctcaa gaagcagaag gcatcctggc gccgcgatcg
     7981 cgagctgaag accgcgctcg ccgccgcgaa cgacacggcc gccaagctca ccacgctcga
     8041 caagcagctc gccaccgcgc agctcgcgct cgcgaccgct cgcaagacag agctcgtcgc
     8101 gatcgatgcc gagctcaagg ccggcccaac ccccgcccgc gtcgatcagc tcaacaagct
     8161 ccgcgctcac ctcacgcccg cgccgatcgc cacgaagaag atcgtgatcc ccgacgccga
     8221 aatcgatccg ctcgcggacc ccgacgatct cgagaagcaa gccgccgcga tcgctgccgc
     8281 cgaaaaggcg ctcgaacgcg aacgcacgag cctcgacaag cagcacggcg acctcgtcgc
     8341 gatggcaaac ctgcgctccg cccacgagcg tgccggcgag ctctcgacgc gcgacgacga
     8401 ccaaccccac cgcaacgctc cgcgcggcgg ccgctccgcc gaagtcggcg cgcaaaacac
     8461 cggcgacgac agcgcaggca gccccgcacc tggcggcggc ggcagcacgg gcggcaacgg
     8521 cggcggctcc ggcgccgaaa ccggcggcgg cggcggatct accggcggcg gctcgacggg
     8581 tggcgacctg ttcgacggca gcaagacaac gacgagcttc gaatccagcg ccgcgatcgc
     8641 gctcggcgaa gtcgtcgacc aatcgacgat cgacggcctc ctccgcgcgc gcaactcggg
     8701 cgaccccaag cagcgcgccg acgccgccgc caaagcccgc gacgccgtgc aaaaacgcct
     8761 cgagctcctg aagaagaagc gcgagatgat cgagaagcgc gcgaaggcgc tgcgcaaggg
     8821 cgcctcgacc cgcacgccgc gtaccgacga gatgctgttc tccgagctgc tcgcgccgcc
     8881 ctcgacgagc gtctcctaaa acctaagtgg cctgaccact aagggacgga cgcaacttcc
     8941 gcaacctccg caacctcgag ggacgg
//
LOCUS       DATLBZ010000398         4304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4937452, whole genome shotgun sequence.
ACCESSION   DATLBZ010000398 DATLBZ010000000
VERSION     DATLBZ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4937452"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="VMZ53_20140"
     CDS             <1..1584
                     /locus_tag="VMZ53_20140"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS30837.1"
                     /translation="IPSNPKELEKYDLVLVSDVPAHLMGAGQMAALDTYVKGMGGGLI
                     MAGGEDSFGSGGYERTTIENMMPVRFDSEKIKEQPDIALILVIDRSGSMQGPKLEAAK
                     ESARVTAEVLSPNDIIGVVAFDSEAQVFVRPTRASNRMRISNDISRLQSGGGTNIYPG
                     LKEAFEILQGTNAKVKHVILLSDGEAPTDGLAELVGDMRSARITVSAVGVQGADRNML
                     SMIADTGEGRLYMVEDIGALPKIFMKETQEAQKSQLVEDLIHVRIAKKVEAIEGTAVE
                     NAPPLHGYVTTKPKPTSETILISDLGEPILARWRYGAGSSVAWTSDVKNRWSTDWIRW
                     GGYPKFWAQVIRSSMRRKVYDSYDLFAKVADGRAQVTVDAIDSGDKFVNELDTELQVI
                     DPATNKVTESVAMSQTAAGRYTADFKIQKYGSYLLKAVHKRNGNTVAESMGSVALSYP
                     LEYLRTSADLEPLKHAAQVSGGHDQAKPADVWNAEGEKVPYTEDLWPYVLIAVVGLFI
                     LDLYAKRVRLFGYRTIKFS"
     gene            complement(1581..2903)
                     /locus_tag="VMZ53_20145"
     CDS             complement(1581..2903)
                     /locus_tag="VMZ53_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30838.1"
                     /translation="MYRLHAIALALLVLGSLESSALADDPPPTPAPPNPADPAQPGAK
                     PIRVAVQCEDPGRTKACPAFLLGIIDANKVLLNSPRAGADVVVYAAANEVALVDRLHL
                     RFVGSVTGAPAAIELDVDVDTRGTDDEQRTQIEPAFLAGLSLFVRARFPDAVKTELVT
                     PADMSETKAVGSPWGVQLQVNGNASYTKKYRSAQMHLALVGRYVTRRFRALVGNFTDF
                     GINRQPPLMLEDGTLVSLDSSQWATRGGAEAIKSFNDTWSLGVGSYTNLEDPKSQFRY
                     TSRSRAALEWDMFPADDPRGNRLAVFYHLGWVVERYNIRNELGERFAQYPIHGIDAVG
                     SVRKDKMSFGLQLESIIQLNHPARRRIISASPFVTLQLGSHVDVNLSFSITQREFPAP
                     DPNAIDPSDYAQLSRLSFAEPLQLNGSLGLSIHWDPSNGVRNDRIESI"
     gene            2972..3493
                     /locus_tag="VMZ53_20150"
     CDS             2972..3493
                     /locus_tag="VMZ53_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30839.1"
                     /translation="MSDLLTVVTQAWLIAMGKQQFPIEPLVDPAQGLVVLDHMTDTAS
                     DDEPGIVSAKKLCGDELAKQLPRIRKDLVAAMKHADTMECHNKPGPPFCSFGFAYEYT
                     TMTHLIMRKADDGHLVLDAIAHLDGGSMNEDAYAEQATWVGNQLRQARAKNCAGEKQT
                     SLTYPKRVANRSR"
     gene            3539..>4304
                     /locus_tag="VMZ53_20155"
     CDS             3539..>4304
                     /locus_tag="VMZ53_20155"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUS30840.1"
                     /translation="MRFGIDFGTTRTVVAAVHDGRHPIVSFDDGGEFRPYLPGIAAMR
                     SGELLLGWNAARALEKGDCEHAVRSIKRVAQGLSPDDEVAELPGITALELVTAFLREL
                     RAALVDRSTVEVRAPFDVMVSVPANAASRQRWLTLEAFRRAGFQPIGMLNEPTAAAIE
                     LAHLHLGELGKRSPKRYVVVYDLGGGTFDTSAVSLEGRRFELIAAEGIARLGGDDFDE
                     HVLDLACAQLGVEPEGAQRAAALEWAREAKETLRSTS"
BASE COUNT          793 a         1375 c         1432 g          704 t
ORIGIN      
        1 atcccgagta atccgaagga gctcgagaaa tacgacctcg tgctcgtcag cgacgttccc
       61 gctcacctca tgggtgcagg ccaaatggcg gcgctcgaca cgtacgtcaa aggcatgggc
      121 ggcggcctca tcatggcggg cggcgaagac tcgttcggct cgggcggcta cgagcgcacg
      181 acgatcgaga acatgatgcc ggtgcgcttc gactccgaga agatcaagga gcagcccgac
      241 atcgcgctca tcctcgtgat cgatcgctcc ggctcgatgc aaggtcccaa gctcgaagcc
      301 gcgaaggaat ccgcgcgcgt cacggcggag gtcctctcgc cgaacgacat catcggcgtc
      361 gtcgcgttcg acagcgaagc gcaagtcttc gtccggccga cgcgcgcgtc gaaccgcatg
      421 cgcatctcga acgacatctc gcgcctgcag tccggcggcg gcacgaacat ctacccggga
      481 ctcaaagaag cgttcgagat cctgcaaggc acgaacgcga aggtgaagca cgtcatcctc
      541 ctctccgacg gtgaagcgcc gaccgacggc ctcgccgagc tcgtcggtga catgcgctcg
      601 gctcgcatca cggtgtccgc cgtcggtgtg caaggcgccg accgcaacat gctctcgatg
      661 atcgccgaca ccggcgaagg tcgtctctac atggtcgaag acatcggcgc gctgccgaag
      721 atcttcatga aggagacgca agaagcgcag aagtcgcagc ttgtcgaaga cctcatccac
      781 gttcgcatcg ccaagaaggt cgaagcgatc gaaggcacag ccgtcgagaa cgccccgccg
      841 ctgcacggct acgtcacgac gaagcccaag ccgacgtccg agacgatcct catctcggat
      901 ctcggcgagc ccattctggc caggtggcgg tatggagcag gctcctccgt cgcgtggacc
      961 tccgacgtca agaaccgctg gtcgacggac tggatccgct ggggcggcta cccgaagttc
     1021 tgggcgcagg tcatccgcag ctcgatgcgc cgcaaggtct acgacagcta cgacctgttc
     1081 gcgaaagtcg ccgacggccg cgcgcaagtc accgtcgatg cgatcgactc cggcgacaag
     1141 ttcgtcaacg agctcgacac cgagctgcaa gtcatcgatc ccgcgacgaa caaagtcacc
     1201 gaatccgtcg cgatgtcgca aaccgccgcc ggccgctaca ccgctgactt caagatccag
     1261 aagtacggca gctacctcct aaaggccgtc cacaagcgca acggcaacac cgtcgccgaa
     1321 tcgatgggct cggtcgccct ctcctacccg ctcgaatacc tccgcacgtc ggccgacctc
     1381 gaaccgctga agcacgccgc gcaagtctcc ggcggtcacg atcaggccaa gcccgccgat
     1441 gtctggaatg ccgagggcga gaaggtgccg tacaccgagg acctgtggcc gtacgtgctc
     1501 atcgcagtcg tcggcctctt catcctcgat ctctacgcga agcgcgtccg cctgttcggc
     1561 taccgcacga tcaagttcag ctagatcgat tcgatgcggt cgttgcggac gccgttgctc
     1621 gggtcccaat ggatcgagag tccgagcgag ccgttgagct ggagcggttc cgcgaacgac
     1681 agccgcgaga gctgtgcgta gtccgatgga tcgatcgcat ttggatcggg cgccgggaat
     1741 tcacgctggg tgatcgagaa cgacagattc acgtcgacgt gcgagccgag ctgcagcgtg
     1801 acaaacggcg aggccgagat gatgcgccgc cgcgcgggat ggttcagctg gatgatgctc
     1861 tcgagctgga gcccgaacga catcttgtcc ttgcgcacgc tgccgacggc gtcgatgccg
     1921 tggatcgggt actgcgcgaa ccgctcgccg agctcgttgc ggatgttgta gcgctcgacg
     1981 acccagccga ggtgatagaa gaccgcgagc ctgttaccac gcggatcgtc ggcgggaaac
     2041 atgtcccact cgagcgccgc gcgagagcgc gacgtgtagc gaaactgcga cttcggatcc
     2101 tcgaggttcg tatagctgcc gacgccgagc gaccacgtgt cgttgaacga cttgatcgcc
     2161 tccgcaccgc cgcgcgtcgc ccactgggag ctgtcgaggg acacgagcgt gccgtcttcg
     2221 agcatcagcg gtggctggcg gttgatgccg aagtcggtga agttgccaac gagcgcgcga
     2281 aagcggcgcg tgacgtaacg accgacgagc gcgaggtgca tctgcgcaga gcgatacttc
     2341 ttcgtgtagc tcgcgttgcc gttcacctgg agctgaacac cccacggtga gccgacggcc
     2401 ttcgtctcgc tcatgtctgc aggcgtgacg agctccgtct tgacggcatc cggaaagcgc
     2461 gcgcggacaa agaggctgag gcccgcgagg aaggccggct cgatctgcgt gcgctgttcg
     2521 tcgtcggtgc cgcgtgtgtc gacgtccacg tcgagctcga tcgccgcggg cgcgccggtc
     2581 acggaaccga cgaagcgcag gtgaagccga tcgacgagcg cgacttcatt tgccgcggcg
     2641 tacacgacga cgtcagcgcc ggcgcgtggc gaattgagga gcaccttgtt cgcgtcgatg
     2701 atgccgagca ggaatgccgg gcacgccttc gtgcgtcctg ggtcttcgca ctgcaccgcg
     2761 acgcggattg gtttggcgcc gggctgcgcg gggtcggcgg gattcggcgg cgctggtgtc
     2821 ggcggcgggt catcggcgag cgcactactc tcgagcgacc cgagcacgag cagcgcgagc
     2881 gcaatcgcat ggagccggta catctcggcg cgagggtcgc ccagtgggcg cggcactgca
     2941 atagacgcgc aacacggcgc gttagatcga catgtccgat ctgctgaccg tcgtgacgca
     3001 ggcgtggctc attgcgatgg gcaagcagca gttcccgatc gagccgctgg ttgatcccgc
     3061 gcaaggactt gtcgtcctcg atcacatgac ggacacggcc agcgacgacg agccaggcat
     3121 cgtctccgca aagaagctct gcggcgacga gctcgcgaag cagctgccgc gcatacgcaa
     3181 ggacctggtt gcggcgatga agcacgccga cacgatggag tgtcacaaca agccgggacc
     3241 gccgttctgc tcgtttggct tcgcgtacga gtacacgacg atgacgcact tgatcatgcg
     3301 caaggctgac gatggccacc tcgtcctcga cgcgatcgcg catctcgatg gtggctcgat
     3361 gaatgaggac gcgtacgccg agcaggcgac gtgggttggc aatcaattac gtcaggcacg
     3421 ggcaaagaac tgcgccggtg agaagcagac gtcgctcacg tatccgaaac gcgtcgcgaa
     3481 ccgatctcgg taggcgcgct cccggctgac gcggcgtcga ttcgtgctac gggttggcat
     3541 gcgcttcggg atcgatttcg gcaccacgcg cacggtcgtg gccgcggttc acgacgggcg
     3601 gcatccgatc gtctcgttcg acgacggcgg cgagtttcgg ccgtacctgc ccgggatcgc
     3661 ggcgatgcgc agcggcgagc tgctgctcgg atggaatgcg gcgcgcgcgc tcgagaaggg
     3721 cgattgcgag catgctgtgc ggtcgatcaa gcgcgtcgcg cagggcctct ctcccgacga
     3781 cgaagtggcc gagctgccgg gcatcaccgc actcgagctg gtcaccgcat tcttgcgcga
     3841 gctgcgcgcg gcgctcgtcg atcgctcgac tgtcgaggtg cgcgcgccgt tcgacgtcat
     3901 ggtgtccgtg ccggcgaatg cggcatcgcg gcagcgctgg ctcacgctgg aagcatttcg
     3961 acgtgcgggt tttcagccga tcggcatgtt gaacgagccg accgcggcgg ccatcgagct
     4021 cgcgcatctg cacctgggtg agctcggcaa gcgaagcccg aaacgctacg tcgtcgtcta
     4081 tgacttgggc ggcggcacgt tcgatacgtc cgcggtctcg ctcgagggac ggcgcttcga
     4141 gctcatcgcg gcggaaggca tcgcgcggct cggcggcgat gacttcgacg agcacgtgct
     4201 cgatctcgcg tgcgcacagc ttggcgtcga gcccgaaggt gcgcagcgtg ccgcggcgct
     4261 cgagtgggcg cgcgaagcga aagagacgct gcgctcgacg agca
//
LOCUS       DATLBZ010000399         6935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4172520, whole genome shotgun sequence.
ACCESSION   DATLBZ010000399 DATLBZ010000000
VERSION     DATLBZ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4172520"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VMZ53_20160"
     CDS             complement(<1..1160)
                     /locus_tag="VMZ53_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30841.1"
                     /translation="MRIGVSLVAIVVSSGVAYAQAVDRRYAEEPTRGLALPTTGLAGE
                     HDARAVVANAGGLPLLRGPEMTLALELEDPEVATSAGPGFGLYVGTAGGGGLLPRYGV
                     GMGFEWLRPSRAQVSPDPGQPFRFTLGMALGLGAKAGFGLAWHHFSAEGNLDGEDTFD
                     LGLSTRWGSRLGIGATLIDVATKPIGGAPVQRRYELEALWRPLATDRLEVGIGGRVGE
                     TRGDLDGWLRLSARIARGVFLLGEVESRQVYALQDSPMGLTEEDGRELRVTAGLEVSF
                     GAMGVTALGSGLRNDLGTNHPLGSTLIVRASGQGPASVLGDGDHIERVELSGTIGSRE
                     LTSIVLRLRSIAHDSTAKAVIVMFDGADGGWATLQELRDELLAVKKAGKKVFA"
     gene            1186..1650
                     /locus_tag="VMZ53_20165"
     CDS             1186..1650
                     /locus_tag="VMZ53_20165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUS30842.1"
                     /translation="MPNPTLLERHPLLVNLSPDQLERLAKVGEVESYNPSENIVVEGS
                     LGDALFLILSGQVAVHKGPQTFATLNAGEFFGEMSLVEPAPRSATVTSMSATFVFRLP
                     HDALRALLEDDPHAASVLLVQVVKTLSDRLRRANQMLSSVDMLADWLAGSMV"
     gene            complement(1651..5157)
                     /locus_tag="VMZ53_20170"
     CDS             complement(1651..5157)
                     /locus_tag="VMZ53_20170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30843.1"
                     /translation="MVGLSSITSTSSPAMCVFPSLRKTAAESTSYVLPISLSPALGGT
                     SSIGDVRATVVASSGVPNREGDWEHYPRKFGKYHLLAPLAQGGMGALYLAVTGDRGLE
                     RMMVIKTVLPHLADAEYVARFRDEAKVVVKLSHGNLIPVFDAGLVGGELFLAMDFVEG
                     RDLRAVWNRCAKKQVAFPIDVAVYIVKELCRGLAYAHSFPDLNLVHRDISPPNVLVSY
                     TGEVKLTDFGLASSNLKLEKTAPGIIYGKVAYMSPEQARGEKLDGRSDMYATGIVLWE
                     LLTGRQLFPPGKDQPQDLLSRAKNPEVMRPSKRAPRVPVELDEICLRALASAKADRYA
                     DCDEMRMALQSWLATNAPTTDGTRMSSFLQELFSEDIVRERNERVEMLAKVRTRALTL
                     PPSDELRQMVEKSDKISIADLGKDDDDDAPVRKPGSVGRRATDREDRPNDRRRESDRR
                     LQAKIAGGKNDSSGALSAAALAQQEEEAPELGIGSIIDGRYKVTELIGEGGMGKVYLA
                     EHVEIGKRVALKVLHPSYSRMPDLVERFRREARAASKIGHPNIVDVTDSGSTPDGSVY
                     FVMEYLEGVELGSIIEREGALDVARALRITTQICRALSAAHREGIIHRDLKPENIYLI
                     TRGSEADFVKVLDFGIAKTTEAEAARERRLTSPGMAMGTPEYMAPEQAAGRPADARTD
                     VYSLGAIMYEMVTGVPPYQGDNFMEILTKKATLDPPPPVSIRTELPQPVSDLVLAAMS
                     RSPDTRPQSMEALEYELNKCLSGRGQAVASILGMTTDANVVATLNPGLSTRNLDSGIV
                     NLPSGGRANTHSEVWDTRSGVSRATNYSSGPVLAHATPSGPIRLPSDPGLGMSPASSG
                     SMARASSPNIHARAGTPIPPDRPSSVSSPIAGEAEALSAEPVQLKRSGMAVFGWIMLI
                     LILVGGGGALAYVMLFQNKQEPTMPAQNPQGSAGSDAQQLDKTNPDKPDKPDKPDKPR
                     KKNDKTPDKTPDKSSAQGSDTTKGSAAVVADTSADTNKGSAAAATTSKTPAKTGKTVA
                     IAEKATVPQLQETAKKAEASGDWDTTLAAYQKLEKAKGYKYPGYAVYKQAWSYFQLND
                     TSNAQTLAQKAATMAGNQKIDAKILYADTWFKLGDYQRAKDFYLGLRKQVAGDKTKSA
                     LIVKKIALCNQKLKKPERDGLD"
     gene            5114..6544
                     /locus_tag="VMZ53_20175"
     CDS             5114..6544
                     /locus_tag="VMZ53_20175"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUS30844.1"
                     /translation="MAGELVLVIDDSPTITKVVQLVLTKAGYQVQTAADGEQGLAAVR
                     SQRPDLILLDFVMPRMNGYQFCRELTADAKLRDIPVILMSAKGDQVGERFVKVMGIVD
                     YITKPFSPEAITAVVQHTIGKYGGPGVEDEHPSLVTGEDLSATDDADKAHAKTGALHQ
                     LRAQVSDAVSARIAALFQLASITSPAGRDEGAKVATDAAAIADALRSALDDATMQRMI
                     SSIDLGLFGEGTPGLRGDLRVVPLAEVLQLLDVQEQSGVLTVERSGARVDIYFRRGRV
                     DQAIAEGVPEEFLLGRFVTDAELMTRGDFESFLESRSLHTGAKLLGAQLVKLGHLADA
                     DLKACLTRQSSELIYEILRWRHGRFRFGAGMELPPPVIDAALGLDVEAVLMEGYRRVD
                     EWHLIERAIDNFDVVFLRNEDSVAQMGRGRLTREELAVLELVNGKNTVKDIVRKSRMG
                     SFEVSKMLYRLLSIKLVRRRVLPVAV"
     gene            complement(6602..>6935)
                     /locus_tag="VMZ53_20180"
     CDS             complement(6602..>6935)
                     /locus_tag="VMZ53_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30845.1"
                     /translation="GMRYALLAALLLACGGSKPPSSDNTPPIGSQGSGAVSDGDCVKT
                     GCSGTICADPGNEVVTTCEMKPEYACYQNATCARQTDGKCGWTQTPELTSCLANPPKL
                     DGGGPAPQ"
BASE COUNT         1124 a         2284 c         2249 g         1278 t
ORIGIN      
        1 gcaaacactt tcttgccggc cttcttcacg gcgagcagct cgtcgcgcag ctcctgcagc
       61 gtggcccatc cgccgtccgc gccgtcgaac attacgatga cggctttcgc cgtcgagtcg
      121 tgcgcgatcg accgcagtcg cagcacgatc gatgtcagct cgcgcgagcc aatggtcccc
      181 gacagctcga cgcgctcgat gtgatcgcca tcgcccaaga ccgatgcggg cccctgcccc
      241 gacgcgcgga cgatcagcgt gctgccgagt gggtgattgg tgccgagatc attgcgcagc
      301 ccgctgccga gcgcggtcac gcccatcgcg ccgaacgaga cctccaagcc ggcagtgacg
      361 cggagctcgc gaccatcttc ttcggtcaat cccatcggcg agtcctgcag cgcatagacc
      421 tgtcgcgact cgacttcgcc gagcaagaag acgccgcgcg cgatgcgcgc cgacagccgc
      481 aaccagccat cgagatcgcc gcgcgtctca ccaacgcgcc cgccgatgcc aacctccaag
      541 cgatccgtcg cgagcggacg ccacagcgcc tcgagctcgt agcgcctctg cacgggcgca
      601 ccgccgatcg gcttcgtcgc gacatcgatg agcgtggcgc cgatgccgag ccggctgccc
      661 catcgcgtcg acaatccgag gtcgaacgtg tcttcaccgt cgaggtttcc ctcggcgctg
      721 aagtgatgcc acgcgagacc aaagcccgcc ttcgccccga gcccgagcgc catgccgagc
      781 gtgaagcgaa acggttgtcc gggatccggc gagacctgcg cgcgcgacgg tcgcaaccac
      841 tcgaagccca tcccgacgcc atagcgcggc aagagcccgc cgccgcccgc agtcccgacg
      901 tacaggccaa agcccggtcc ggcgctcgtc gcgacttctg gatcctcgag ctctagcgcg
      961 agcgtcatct cgggtccgcg caacagcggc agcccgcccg cattcgcgac gacggcgcgc
     1021 gcatcgtgct cgccggccaa ccccgtcgtc ggcaacgcaa gaccgcgcgt cggctcctcg
     1081 gcgtaccgcc gatctacagc ctgggcgtac gccactccgc tactgacgac gatcgcgaca
     1141 agcgagacac cgatccgcat gggccgcgac tatactgcgg ccggaatgcc gaacccgacc
     1201 ttgctggagc gacatccact cttggtcaac ttgtcccccg accagctgga gcgcctcgcg
     1261 aaagtcggcg aggtcgagag ctacaacccg tcggagaaca tcgtcgtcga gggatccctc
     1321 ggtgacgccc tgttcctcat cctctcgggg caggtcgccg tccacaaagg cccgcagacg
     1381 ttcgcgacgc tcaatgccgg tgaattcttc ggcgagatgt cgctcgtcga gcctgcgccg
     1441 cggtcggcga cggtcacctc gatgtccgcg acgttcgtgt tccgcctgcc gcacgacgcg
     1501 ctgcgcgcgc tgctcgaaga cgatccgcac gccgcgtcgg tgttgctcgt gcaggtcgtc
     1561 aagacgttgt cggaccggct gcgccgcgcg aatcagatgc tgtcgtcggt cgacatgctc
     1621 gctgactggc tcgccggctc gatggtctga tcagtcgaga ccgtcgcgct cgggcttctt
     1681 cagcttctga ttgcagagcg cgatcttctt tacgattaga gcgctcttcg tcttgtcacc
     1741 cgcgacttgc ttgcgcaggc ccagatagaa gtccttcgcg cgctggtaat cgccgagctt
     1801 gaaccacgta tccgcgtaga ggatcttggc gtcgatcttc tggttgcccg ccatcgtcgc
     1861 ggccttttgc gcgagcgttt gggcgttgct cgtgtcgttg agctggaagt acgaccacgc
     1921 ctgcttgtag acggcgtagc cgggatactt gtagcccttc gccttctcga gcttctgata
     1981 cgccgcgagg gtcgtgtccc agtcgccgct ggcttccgcc ttcttcgcgg tttcctgcag
     2041 ctgcgggacg gtcgccttct cggcgatcgc gacggtcttg cccgtcttcg ccggcgtctt
     2101 cgacgtcgtc gcggcagcgg cgctgccttt gttcgtgtcg gccgacgtat cagcgacaac
     2161 cgccgcgctg cccttggtcg tatcgctgcc ttgggcagac gacttgtcgg gcgtcttgtc
     2221 gggcgtcttg tcgttcttct tgcgcggctt gtccggcttg tcgggtttat ccggcttgtc
     2281 cgggttcgtc ttgtcgagct gctgcgcgtc ggagcccgcg gagccctgcg ggttctgcgc
     2341 tggcatcgtc ggctcttgct tgttctggaa cagcatcacg tacgcgagcg cgccaccgcc
     2401 gccgacgagg atcaggatga gcatgatcca gccgaacacg gccatgccgc tgcgcttgag
     2461 ctgcacgggc tcggcggaga gagcctctgc ttcgcctgcg atcggtgacg agaccgacga
     2521 cgggcggtcc ggcggaatcg gcgtgccggc gcgagcgtgg atgttcggcg acgacgcgcg
     2581 cgccatcgat ccactcgacg ccgggctcat cccgaggccc ggatcgctcg gcaggcgaat
     2641 ggggcctgac ggcgtcgcgt gcgcgagcac cggaccggag ctgtagttcg tcgcgcgcga
     2701 cacacccgag cgcgtgtccc agacttcgct gtgcgtgttc gcgcgaccgc cggacggaag
     2761 attgacgatg ccgctgtcga ggttgcgcgt cgacaggccc gggttcagcg tcgcgacgac
     2821 gttcgcgtca gtcgtcatgc cgaggatcga ggcgacggct tggccgcgac ccgagaggca
     2881 cttgttgagc tcgtactcga gcgcttccat cgattgcggg cgcgtatccg ggctgcgcga
     2941 catcgccgcg aggacgagat cgctcaccgg ttgcggcagc tcggtgcgaa tcgagacggg
     3001 aggcggcgga tcgagcgtcg ccttcttcgt caggatctcc atgaagttgt cgccttggta
     3061 cggcggaact ccagtgacca tctcgtacat gatcgcgccg agcgagtaga cgtcggtgcg
     3121 cgcgtcggcg ggacgaccgg cggcttgctc gggcgccatg tactcgggcg tgcccatcgc
     3181 catgccgggg ctggtgaggc gacgttcgcg tgcagcttcg gcttcggtcg tcttcgcgat
     3241 accgaagtcg agcactttca cgaagtcggc ttcggagccg cgcgtgatga gatagatgtt
     3301 ctcgggcttg aggtcgcgat ggatgatgcc ttcgcggtgg gcagcgctca gcgcgcggca
     3361 gatctgcgtc gtgatgcgca gcgcacgcgc gacgtcgagg gcgccttcac gctcgatgat
     3421 ggagccgagc tcgacacctt cgaggtactc catgacgaag tacacgctgc cgtcgggggt
     3481 agagcccgag tcggtgacgt cgacgatgtt cgggtgaccg atcttcgatg cggcgcgcgc
     3541 ctcgcggcgg aatcgctcga cgagatcggg catgcgcgag tagctcggat gcaagacctt
     3601 cagcgcgacg cgcttaccga tctcgacgtg ctcggcgagg tagaccttgc ccatgccgcc
     3661 ttcaccgatc agctcggtga ctttgtagcg accgtcgatg atcgagccga tgccgagctc
     3721 gggcgcttct tcttcttgct gcgcgagggc ggcggcagag agcgcacccg acgagtcgtt
     3781 cttgccgcca gcgatctttg cttggagacg gcgatccgat tcacgacgac gatcgtttgg
     3841 acgatcttcg cggtcggtcg cgcgacgccc gacggagccc ggcttgcgaa ccggcgcgtc
     3901 atcgtcgtca tctttgccga ggtcggcgat cgagatcttg tcggacttct cgaccatctg
     3961 gcgcagctcg tcgctcggcg gcaacgtcag cgcacgcgtg cggaccttcg cgagcatctc
     4021 gacgcgctcg ttacgctcgc ggacgatgtc ctcggagaac agctcctgga ggaacgacga
     4081 catgcgcgtt ccatcggtgg tcggcgcgtt tgtcgcgagc cagctctgca gcgccatgcg
     4141 catctcgtcg caatcggcgt agcgatccgc tttcgccgag gcgagtgcgc ggaggcaaat
     4201 ctcgtcgagc tcgacgggaa cgcgcggcgc acgcttgctc gggcgcatca cctcggggtt
     4261 cttcgcgcgc gacagtagat cttgcggctg gtctttgccc ggcgggaaca gctggcgacc
     4321 agtgagcagc tcccagagca cgatgcccgt cgcgtacata tccgagcgac cgtcgagctt
     4381 ttcgccgcgc gcctgctcgg ggctcatata cgcgaccttg ccgtagatga tgccgggcgc
     4441 cgtcttctcg agcttgaggt tcgacgacgc gagaccgaag tcggtcagct tcacttcgcc
     4501 ggtgtacgag acaagaacgt tcggcggcga gatgtcgcga tggacgagat tgagatcagg
     4561 aaacgagtgc gcgtacgcga gaccgcgaca cagctccttc acgatgtaga cagcgacatc
     4621 gatcgggaac gcgacctgct ttttcgcaca gcgattccag actgcgcgga ggtctctgcc
     4681 ctccacgaag tccattgcaa gaaacagctc gccaccaact agaccggcgt cgaacacggg
     4741 aatcagattc ccgtgtgaca gcttcaccac aactttcgct tcatcgcgga atcgcgcgac
     4801 gtactccgcg tctgcgagat gcggcaaaac ggtcttgatg accatcatcc gctcgagtcc
     4861 gcggtcgccg gtcaccgcga gataaagcgc gcccatgcca ccttgggcca agggggcaag
     4921 cagatgatac ttgccgaatt tcctggggta atgctcccag tctccctcgc ggttgggcac
     4981 gcctgaagac gctaccacgg tggcccttac gtcaccaatc gaggaagtac ccccaagtgc
     5041 cggtgacaat gaaattggga gcacataaga tgtcgactcc gctgctgtct tgcggagtga
     5101 gggaaagacg cacatggccg gcgagctggt gctcgtaatc gacgacagcc cgaccattac
     5161 aaaggtcgtg caactggttc tgacgaaagc cggctaccaa gtgcagacgg ctgccgacgg
     5221 agaacagggc cttgcggcgg ttcgctcgca acgtccggac ctcatcctcc tcgacttcgt
     5281 catgccgagg atgaacggct accagttctg tcgagagctg acggccgacg ccaagctccg
     5341 ggacatcccg gtgatcctga tgtccgcgaa gggggatcag gtcggcgagc ggttcgtgaa
     5401 ggtgatgggg atcgtcgact acatcaccaa gccgttctcg cccgaggcga tcaccgcggt
     5461 cgtgcagcac acgatcggca agtacggcgg ccccggcgtc gaggacgagc atccatcgct
     5521 cgtcacggga gaagatctct ccgcgacgga cgatgccgac aaggcgcacg ccaagaccgg
     5581 cgcgcttcac cagctgcgcg cgcaggtctc cgatgcggtc agcgcgcgga tcgccgcgct
     5641 ctttcagctt gcatcgatca catctccggc cggccgtgat gagggtgcga aggtcgcgac
     5701 cgatgcggcg gcgattgccg atgcgctgcg cagtgcactc gacgacgcga cgatgcagcg
     5761 catgatcagc tcgatcgatc tgggcctgtt cggcgaaggc acgccaggcc tccgcggcga
     5821 cctgcgcgta gttccactcg cagaagtctt gcagctcctc gacgtgcaag agcagtcggg
     5881 cgtgctgacc gtcgagcgca gcggcgcgcg cgtcgatatc tacttccgcc gcggccgcgt
     5941 cgatcaagcg atcgccgaag gcgtgcccga agaattcctg ctcggtcggt tcgtcaccga
     6001 tgcggagctg atgacgcgcg gcgatttcga gtcgttcctc gaatcgcgat cgctccacac
     6061 cggcgcgaag ctgctcggcg cccagctcgt caagctcggc catctcgccg acgcagatct
     6121 gaaggcgtgc ctcacccgcc agtcgagcga gctcatctac gagatcctgc gctggcgcca
     6181 cggccgcttc cggttcggcg cgggcatgga gctgccgcca ccggtgatcg atgctgcgct
     6241 cggcctcgat gtcgaagctg tgctgatgga aggctaccga cgcgtcgacg agtggcatct
     6301 catcgagcgc gcgatcgaca acttcgatgt cgtcttcctg cgcaatgaag actccgtcgc
     6361 gcagatgggg cgcggccggc tcacgcgcga ggagctcgcc gtgctcgagc tcgtgaacgg
     6421 caagaacacc gtcaaagaca tcgttcgcaa gtcgcgaatg ggcagcttcg aagtcagcaa
     6481 gatgctgtac cgcctgctca gcatcaagct ggtgcgtcgt cgcgtgctgc ccgtcgccgt
     6541 ctgatcgtcc tgcgcaacgc ttagcgttgc tgcgcggtcc agcgaagctg gaccttgctc
     6601 gttactgcgg cgctgggcct ccgccgtcga gcttcggcgg attcgcgagg cagctcgtca
     6661 gctccggcgt ttgggtccag ccgcacttgc catcggtttg ccgcgcgcag gtcgcgtttt
     6721 ggtagcaggc atactctggt ttcatctcgc aggtcgtgac gacctcgttg cccgggtcgg
     6781 cgcagatcgt gccgctgcag ccggttttca cgcaatctcc gtcggagacg gcgcccgatc
     6841 cttgggaccc aatgggtggc gtgttgtcgg atgacggcgg ctttgatccg ccgcatgcga
     6901 gcagaagagc tgcgagtagt gcgtagcgca tgccg
//
LOCUS       DATLBZ010000400        13322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4175420, whole genome shotgun sequence.
ACCESSION   DATLBZ010000400 DATLBZ010000000
VERSION     DATLBZ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13322
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4175420"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VMZ53_20185"
     CDS             complement(<1..930)
                     /locus_tag="VMZ53_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30846.1"
                     /translation="MRGTLRHVAAFAILWVASACGTGDTKVDPADLELRDLLGVAPEV
                     ATTWDAEQRSSARRVLVDALDDQKGAPSETLDAYRSDDDLVRVLAIADAKRLDAGDDA
                     LGLVRIRGAQLSTLRGSRFTERAKELTSSEMVIDALASDSGLVDARGLVVVREPRLTV
                     AAAYVEGTPPRLLVNTVVLSAFDEGGVSLESKRGGAVGGGGSGDDVAKPGAKAIAANG
                     NPYSFYGSVAECAYAQRLRCEACLPSSSCTEAITSASGMDECTKLAENDGRGYFLLCI
                     NLALAITSVDQCTADAVPSCPRTTTAANDLGQIE"
     gene            complement(1042..2433)
                     /locus_tag="VMZ53_20190"
     CDS             complement(1042..2433)
                     /locus_tag="VMZ53_20190"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS30847.1"
                     /translation="MRKHVAFAIVVVLSACGTEPGVGGDDTSNPYPEVPSEQQRSGDP
                     AKGYDYLLNGGYVTCGLPASIAPRGSAADRIPGRTGDNATLPYYWSAATSMEGVRVVS
                     ANCLMCHAGRINGQVVVGLGAADVDFTKSQLTLVNAAGGLINDPTEKMEWERFRARLA
                     AINDWQQTLTIGTNPADSFTAALMAHRDPKTLAWSDEPLVALPPKVVIPVDTPPWWRM
                     NKKHAMFYTAAGRGDHARIMMAASLLCTESIEEATKIDQAFVDVRAWIEEGLAAPKWP
                     WSVDMSLAAKGQTVFEATCSKCHGTYGAGGKYPNQLVPLADVGTDATLASGETEFAGR
                     YVEWFASSFWGETSRMEPQQGYIAPPLDGIWATAPFFHNGSVPTLEGVLEPSARPTYW
                     TRTMESTDYNQGAVGWNYTKLDHGQAAEPTLNARVKIYDTTLVGYGNGGHSFAEDLSG
                     DDKLALLEYLKTL"
     gene            2466..3200
                     /locus_tag="VMZ53_20195"
     CDS             2466..3200
                     /locus_tag="VMZ53_20195"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HUS30848.1"
                     /translation="MLLNLDAGEHDHEPEELWTLADILAVACGGHAGDAASMSRLAAF
                     VARSSRRTALGAHPSYPDRANFGRTSIAIEPAVLEAEIAAQCRALANVARSYGVSVHY
                     VKPHGALYHDAARNRVIANAVIDGALVGTAIDDGGASPVADALAAARNAQIAFVGPPT
                     GAFADAVRIRGLAYLREGFADRAVKSDGSLVPRSEPNALITDPAAAVARARELAGHVD
                     TICVHGDSPGALDIARAVRTLLARSV"
     gene            3273..3458
                     /locus_tag="VMZ53_20200"
     CDS             3273..3458
                     /locus_tag="VMZ53_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30849.1"
                     /translation="MTTNLAISRRGRRRVAIPRRAATPILSRWAAAPMSRDGPPRRSL
                     VMGGRADSLAMGRRADL"
     gene            3572..4276
                     /locus_tag="VMZ53_20205"
     CDS             3572..4276
                     /locus_tag="VMZ53_20205"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HUS30850.1"
                     /translation="MSDSTTSVPTKVLPTTFAPTRSAPTWQRLGDRAIRFARPSAPVS
                     ARELVRAVRAWPGVVDVVVAAEDVAAYFVDVLALEAMQQQLARHIEELATLVDGDELV
                     REHVLRVVYDGADLDDVARAKRLSREEVIALHSGATYTVDTIGFAPGFAYLAGLDARL
                     ELPRRATPRPRVPAGSVAIAGRQSAVYPFDSAGGWHLVGRVVGVSMFGENGALLSLGD
                     RVRFVGAEKPTDVGPK"
     gene            4288..5130
                     /locus_tag="VMZ53_20210"
     CDS             4288..5130
                     /locus_tag="VMZ53_20210"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30851.1"
                     /translation="MRTLGLCTVQDLGRRGHMHEAVPPGGALVPELLVAANRRVGNRD
                     DAPALEVLGRLVVRAASDVHIATHRESCELRPGGEHVVESAPLRAAYLAVRGGFDVPV
                     RLGGRGTLLCARLGRPLARGDVLRVGTEARGVGGVGDHGTSRESDRFVGSDDVAVIPG
                     PDLDAFESDAIEELVRATYRISPTSDRVGTRLEDLGAPSAGSRASSRNSSGLRCVEGY
                     RERSRPMVIGALEVPRDGQPIVLGPEHPTTGGYPVIAVIASAELGRFFAIPLGGTVRF
                     RVAR"
     gene            complement(5157..6308)
                     /locus_tag="VMZ53_20215"
     CDS             complement(5157..6308)
                     /locus_tag="VMZ53_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30852.1"
                     /translation="MRFVLACLLAASLPACVATPQGDDHSPPAGDDNPADPTDTADPS
                     DPSDPADPSDPSDPADPSDPSDPSDPSDPSDPSDPTPPPPPVASGAYSVHSNVDLTVE
                     ALLPQSAEDVVLTLRSFSTNPGGTLLDLADDAGVPAVQEIRDDLPDYVEDKLIGWING
                     EIAKVTINGTPITQIAGATASLAETALTQFALDSQLTIQSDGTATHELTTLDLTPAGI
                     DKTFSLADLPSDMRSQSPTCTSSNGALAIGDHTYGLPYGDYIWTAINEQMVAQYGYDL
                     RGALGAAVNCPSLAHTIANKCVWGYCVGHEAQLNQICEAGLDEAVSRMHDKLAEENFE
                     AMHFASGSATLVDANADGIADSLASGTWDAEINAGQGLRHVPATFTATR"
     gene            complement(6459..6902)
                     /locus_tag="VMZ53_20220"
     CDS             complement(6459..6902)
                     /locus_tag="VMZ53_20220"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUS30853.1"
                     /translation="MASYEKRTWWSKHELTDRLKDETFLEAAVTCAFLVAAADGNQSE
                     QEYDALLDRLEILGGVDRDKIDELLTDASHDVEERGFEPRIARVAELLSGKHAHAEAA
                     LMLGMAIALADDDVSKEEREVATQLSAALGLGLLTLDGMIAEIRG"
     gene            6994..8670
                     /locus_tag="VMZ53_20225"
     CDS             6994..8670
                     /locus_tag="VMZ53_20225"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HUS30854.1"
                     /translation="MRTTFLLVASLLIACGPGARHNGDDDDGPDSATCQNACSVDGHA
                     IVDCHNNVVTQCEGAAACDPATFTCADACTAAESNHRSVGCDYYATDMDSLFGGYCYA
                     VFVANTWTSPAHINVDYKGQQLNVATFTRIPSGSGPTLTQGTFDPAAGLAPGQVAILF
                     LGGTQGAAPNCPVTPAAAAPSLSGTGISDAFHIKTDVPVVAYQINPYGGGSVAVTAAS
                     LLLPTSVWDQEYVAVNVSAASAGNPSMNIVAKEDGTTVTLTPNAALSGAGGIPSGAAG
                     SPVTINLNKGQHAQITQATELTGSTVQSNKPVGFMAGHTCMNMPVGTSYCDHGEQMVA
                     PVQALGSRYVGVMYRPRVAQETGTYWRVIGAVDGTQLTYSTNVGGPTTLAKGQQVLFQ
                     TGTPFTVESQDNDHPFMLFTYMTSSNAVSDGYGDPDFVTSVPPEQYLQNYVFFTDPTY
                     PETNIVMVRARSQIDNNFHDVMLDCAGPITGWQPIGNGEYEYARKDLMTGNFTSVGTC
                     STGRHEIKSDAPFGLWVWGWGTPLTSTFTANVSYGYPGGMNVSPINSVIL"
     gene            complement(8667..8897)
                     /locus_tag="VMZ53_20230"
     CDS             complement(8667..8897)
                     /locus_tag="VMZ53_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30855.1"
                     /translation="MTEPALYDLVRELEPVTRRRDPELEVDIEVEDDCLLDPWFMRSE
                     AEIAQIEDDPTARIHEIRITGYTKLTEDYAVF"
     gene            9036..9287
                     /locus_tag="VMZ53_20235"
     CDS             9036..9287
                     /locus_tag="VMZ53_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30856.1"
                     /translation="MDLTLDELASVRGGYSDADFGRCGPGSHLKFLGDVRTSECAAHD
                     GAVRGALANGSSQVGAQLRALPLLPAAVGSYFRARFTGK"
     gene            9367..10419
                     /locus_tag="VMZ53_20240"
     CDS             9367..10419
                     /locus_tag="VMZ53_20240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUS30857.1"
                     /translation="MPRQSDRYALDLVVELYAQGEWRAVALQDVSRTGMFLATNAPIA
                     AGTPVIVAFTDDEDMRVACQARVTHFLPVADAETLGRIPGVGIVFREPQDVAFSAAVE
                     SVLRRARTRQPEHSHIVVGAAEHRLLERLSTALGGAGFSVATATNGLEFWSACMRRLP
                     DVVLIDREMPILDGPTIIERLACDDQLASVPSILMTKDAGELGPALARGAADVILKPF
                     TLLELIARARRVAQSPKRTERVALSGSLADINLGALLTLLEQQRQTGRIVLSNGHAAW
                     IDILDGKIVDAGWSRGSAHPRAVMMELLDWSHGTFKLTPPPTRRRDSDLRLPITHLLL
                     EQARLRDEANHRKAIG"
     gene            complement(10398..11015)
                     /locus_tag="VMZ53_20245"
     CDS             complement(10398..11015)
                     /locus_tag="VMZ53_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30858.1"
                     /translation="MNRQHGGIGMYLVFSVVISIGAGYGVYRWMHDNKSMWQKSDDAA
                     TTRTAPAGSKKPANAAVTVPVPAPEPAVAVPDEADPIAEESPAVLAASRARDAIVDAE
                     SDGSPVFGTPGIAGGIEAGSVGRRFRPRAEMLQRCFERNGGGESGSVRVTLMVEPTGK
                     ITQIRVDDRWGEGFAACVMTALNGVTFSATSDRESAVVVQPIAFR"
     gene            complement(11012..11539)
                     /locus_tag="VMZ53_20250"
     CDS             complement(11012..11539)
                     /locus_tag="VMZ53_20250"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HUS30859.1"
                     /translation="MSTEYSFETVFEAPGLTAETIGAAYFDPDHLAAQDVVGQLSERA
                     VTESREDEATRFCSWTVRSLRPLPALVKPFVEGGRLKYVETMTWRKRDNEIDMTIVPQ
                     IGGGRVQIAALYTFTQVGEGRVRRRYKGTINVNVSLLSGKIERAVLAEMEKGMASMSD
                     CTQKWLASAAARSPK"
     gene            11592..12341
                     /locus_tag="VMZ53_20255"
     CDS             11592..12341
                     /locus_tag="VMZ53_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30860.1"
                     /translation="MATLVACASPGADDDLGIDDPSGEGGKDDNGSSRRFTEVDATHT
                     NQTFRTYIERALDELAASDREVARYTAQSIADGRVRIDELSDLTCADFLHVINDLPDL
                     HLKPSDYASLKRRGNPTTAAISAELDGYMWSNRIYVARGMSAQHVAATLVHETNHVVN
                     RSEVGYYDDLPTSAFIHEYRAFYVERTFDPDSYAGIDLVDYVITNYELDRSKIRPEIL
                     AHPLTPKLLPTPAAWQARDVSSDVPEPATCQ"
     gene            12287..12820
                     /locus_tag="VMZ53_20260"
     CDS             12287..12820
                     /locus_tag="VMZ53_20260"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HUS30861.1"
                     /translation="MASSRRLIRRPGARHVPIKAPSKTYRTTIFRDGSMCYIPVAFDP
                     KPVFGKIRAPVKVTVNGFTFASTISPMGHDGPCIPFRRSNREAAGLEGGETLDVTVAL
                     DTSERKVTAPADLTRALRAAKLTARWAELSYTHQKEHVDAIENAKAPETRARRIAAAI
                     KMLAAATPSRKRQSRGT"
     gene            complement(12783..>13322)
                     /locus_tag="VMZ53_20265"
     CDS             complement(12783..>13322)
                     /locus_tag="VMZ53_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30862.1"
                     /translation="EEDKPAVSETKPPPPPPKKALTPEEMGKCELKVSGAVTAEQTTF
                     GGRPATNVTYWMTEEEKKNLMGVDGFAVNCSGKDIKFSMTPIGKPELMPFAPKKYVFK
                     GGHSDGASVNVLFPALGQTATLGDPNGTIDVTAFDKKHIAGTISLSGKFVGKGAKGNV
                     TMSGSFDFKCPGFGACETE"
BASE COUNT         2232 a         4559 c         4178 g         2353 t
ORIGIN      
        1 ttcgatctga ccgaggtcgt tcgccgccgt cgtggtgcgt gggcagctcg gaaccgcatc
       61 ggccgtgcac tgatcgaccg acgtgatcgc gagcgcaagg ttgatgcaga ggaggaagta
      121 tccgcggcca tcgttctcgg caagtttcgt gcactcgtcc atgccggacg ccgacgtgat
      181 cgcttcggtg cacgagctgc tcggcaagca cgcttcgcaa cggagccgct gtgcatatgc
      241 acattcagcg accgagccat agaacgagta cggattgccg ttcgctgcga ttgccttcgc
      301 accgggcttc gcaacatcgt caccgctgcc gccaccgccc acggctccac cgcgcttcga
      361 ctcgagcgac acaccgcctt cgtcgaatgc cgacaagaca accgtgttga cgagcaaccg
      421 cggtggcgtg ccttccacat acgctgcagc gacggtcagc cgaggctcgc gaaccacgac
      481 gagaccgcgc gcatcgacga gcccgctatc ggacgcgagc gcatcgatga ccatttcgct
      541 cgatgtcagc tccttcgctc gctcggtgaa acgcgatccg cggagcgtgc tgagctgcgc
      601 gccacgaatg cgcacgaggc cgagtgcgtc gtcgcctgca tcgaggcgct tcgcatcggc
      661 gatcgcgagc acgcgaacga gatcgtcgtc ggagcgatac gcgtcgagtg tctcgctcgg
      721 cgcgcccttc tgatcgtcga gcgcgtcgac gagcacgcgg cgcgcagagc ttcgctgctc
      781 cgcgtcccac gtcgtcgcga cctccggcgc aacgccgagc aaatctcgaa gctcgagatc
      841 tgctggatcg accttggtgt ctccggttcc gcacgcagac gcgacccata agatcgcgaa
      901 cgccgcgacg tggcgtaatg tcccccgcat acgactcatc ctcgtcgacg aacgggcgag
      961 acgccagtaa gcggccggtg agtcggaggt ttgccggact caccctgcta ggtcgaaccc
     1021 tcgagtgagg gctcctcacg ttcacagtgt cttcaagtac tcgagaagcg caagtttgtc
     1081 gtcgccgctg agatcttcgg cgaacgagtg cccgccgttg ccgtacccga cgagcgtcgt
     1141 gtcgtagatc ttcacccgcg cgttgagtgt gggttctgca gcctggccgt ggtcgagctt
     1201 cgtgtagttc cagccgaccg cgccctgatt gtagtcagtc gactccatcg tgcgcgtcca
     1261 ataggtcggt cgcgcgctcg gctcgagcac gccttcgagc gtcggcacgg agccattgtg
     1321 gaagaacggc gctgtcgccc aaatgccgtc gagcggcggc gcgatgtagc cctgttgcgg
     1381 ttccatgcgc gacgtctcgc cccagaacga cgatgcaaac cactccacgt accgccccgc
     1441 aaactcggtc tcgcccgacg cgagcgtcgc atcggtccca acgtcggcga gcggaacgag
     1501 ctggttcgga tacttcccac cggcgccgta cgtcccgtga cacttactgc acgtcgcctc
     1561 gaacaccgtc tggcccttcg cggccagcga catatcaacg gaccacggcc acttcggcgc
     1621 ggccagtccc tcttcgatcc acgcgcggac gtcgacgaac gcttggtcga tcttcgtcgc
     1681 ctcctcgatc gactcggtac acagcagcga cgccgccatc atgatgcgcg cgtgatcgcc
     1741 acggcccgca gccgtataga acatcgcgtg cttcttgttc atgcgccacc acggcggcgt
     1801 atcgacggga atcacgacct tcggcggtag cgcgacgaga ggctcgtccg accacgcaag
     1861 cgtcttcgga tcgcgatgcg ccatgagcgc cgcagtgaag ctatcggcag gattcgtccc
     1921 gatcgtcagc gtctgctgcc aatcgttgat cgcggcgagc cgagcgcgga accgctccca
     1981 ctccatcttc tccgtcgggt cattgatgag cccgccggcc gcattcacga gcgtcagctg
     2041 tgacttcgtg aagtcgacat ccgccgcacc gagcccgacc accacctgcc cattgatgcg
     2101 gcccgcatgg cacatcaaac agttcgcgct gacgacgcgc acgccttcca tcgaggtcgc
     2161 cgcgctccag taatacggca gcgtcgcgtt gtcccccgtg cgtccaggaa tgcgatctgc
     2221 tgccgaccca cgcggcgcga tcgacgcagg aagaccacac gtcacatacc cgccgttcag
     2281 caggtagtca taccccttcg cgggatcgcc actccgctgc tgctcgctgg gaacctcggg
     2341 atatggattc gacgtgtcgt cgcctccaac tccaggctcg gttccacacg cagaaagaac
     2401 gacgacgatc gcaaaggcga cgtgcttcct cacggccggg gaccctacac tagtcgccag
     2461 ccgcaatgct gctgaatctt gatgccggcg agcacgacca cgagcccgaa gagctgtgga
     2521 ctctcgcgga catcctcgcg gtcgcatgcg gcggccacgc cggcgacgca gcctcgatga
     2581 gccgcctcgc ggccttcgtc gcgcgctcct cacgccgcac ggcgctcggc gctcacccgt
     2641 cgtatcccga ccgcgccaac ttcggccgca catcgatcgc gatcgagccc gcagtgctcg
     2701 aagccgaaat cgccgcgcaa tgccgcgcgc tcgccaacgt cgcccgctcg tacggcgtgt
     2761 ccgtccacta cgtgaagccg cacggcgcgc tctatcacga cgccgctcgc aatcgcgtga
     2821 tcgcaaacgc ggtgatcgat ggtgcgctcg tcggcaccgc gatcgatgat ggcggcgcat
     2881 cgcccgtcgc cgatgctctc gcggcggcgc gcaacgcgca gatcgcgttc gtcggtccgc
     2941 cgaccggcgc attcgccgat gccgtgcgca tccgcggcct cgcctacctg cgcgaaggct
     3001 tcgccgatcg cgcggtgaaa tcggacggct cactcgtccc gcgcagcgag ccgaacgcgc
     3061 tgatcaccga cccggccgcg gcggtcgccc gcgcgcgcga gctcgccgga cacgtcgaca
     3121 cgatctgcgt gcacggcgac tcgcccggcg ccctcgacat cgcacgcgcc gttcgcacgc
     3181 tgctcgcccg ctctgtctag ctccgataaa aggtcgacga gcagatcccg gtgatcatgg
     3241 ggtctctttt cgaaacccgt catgacgccg tcatgacaac taacctcgcg atttctcgtc
     3301 gcggccgccg ccgcgtcgcg attcctcgtc gggcggccac gccgattctc tcgcgctggg
     3361 ccgccgcgcc gatgtctcgt gatgggccgc cgcgccgatc tctcgtgatg ggcggccgcg
     3421 ccgattctct cgcgatgggc cgccgcgcgg atctctagtg tcgtcgccga tcgagagtcc
     3481 gcgcacttgg acgtcatgac ggcgtcatga cgtgatccga aaagacaccc cgatcatccc
     3541 gggatctctt caggcgaggc aggctcgata ggtgtccgac tcgacgacga gcgtgccgac
     3601 gaaggtcctg ccgacgacgt tcgcgccgac gaggtccgcg ccgacatggc agcgcttggg
     3661 cgatcgtgcg attcggttcg cgcgaccgtc ggcgcccgtg tctgcgcggg agctcgtgcg
     3721 agccgttcgt gcctggccgg gcgtcgtcga tgtcgtggtc gcggccgagg acgtcgccgc
     3781 gtacttcgtc gatgtgctgg cgctcgaggc gatgcagcag cagctggcgc gccacatcga
     3841 agagctcgcg acgctcgtcg acggcgatga gctcgtccgc gagcacgtgc tgcgcgtcgt
     3901 ctatgacggc gcagatctcg acgacgttgc gcgcgcaaag cggctctcgc gcgaagaggt
     3961 catcgcactt cattcgggcg cgacgtacac cgtcgacacg atcggctttg cgccgggctt
     4021 tgcgtatctc gcgggcctcg atgcacggct cgagctaccg cgtcgcgcaa caccgcggcc
     4081 gcgcgtgccg gcaggaagcg tcgcgatcgc gggtcggcag agtgcggtct atccgttcga
     4141 ctcggcgggt ggctggcatc tcgtgggccg cgtcgtcggt gtgtcgatgt tcggtgagaa
     4201 cggcgcgctg ctctcactcg gtgaccgcgt gcgcttcgtc ggcgcggaga agccaaccga
     4261 cgtggggcca aaatgacgat cgagattgtg cgaacgctcg ggctgtgcac cgtgcaggac
     4321 ctcggacgtc gcggccacat gcacgaggcc gtgccgcccg gaggtgcgct cgtgcccgag
     4381 ctcctcgtcg ctgcgaatcg acgcgtcggt aatcgcgatg atgcgcccgc gctcgaagtg
     4441 ctcggtaggc tcgtcgtgcg cgctgcgtcc gatgtgcaca tcgccacgca ccgcgagtcg
     4501 tgcgagctgc gccctggtgg cgagcatgtg gtcgagagcg cgccccttcg tgcggcgtat
     4561 ctcgcagtgc gcggaggctt cgatgtgccc gtgaggctcg gtggccgcgg gactctcctg
     4621 tgcgcgcgcc tcggtcggcc gctcgcgcgg ggcgatgtgc tgcgtgtcgg cacggaggcc
     4681 cgcggcgtcg gtggcgttgg cgaccatggg acttcgcgtg agagcgaccg attcgtcggg
     4741 agcgatgacg tcgccgtgat tccaggtccc gacctcgatg ctttcgagtc cgacgctatc
     4801 gaggagctcg tgcgcgcgac gtatcggata tcgccgacga gcgaccgcgt cggcacgcgc
     4861 ctcgaagacc tcggcgctcc aagtgcgggt tcgcgtgcga gctctcgcaa ctcgagcggg
     4921 ctacggtgcg tcgaggggta tcgcgagcgg tcacggccga tggtcatcgg cgcactcgaa
     4981 gtcccgcgcg acggccagcc catcgtcctc gggccggaac atccgacgac cggcggctat
     5041 cccgtcatcg ccgtgatcgc gagcgccgag ctgggccgct tctttgcgat cccactcggc
     5101 ggcaccgtgc gatttcgagt cgcgcggtag tcgagcctct tcgagttgcg ccgcgattag
     5161 cgcgttgcgg tgaacgtcgc cggaacgtgg cgcaggcctt ggcccgcatt gatctcggca
     5221 tcccacgtgc cgctcgcgag cgagtccgcg atgccgtccg cgtttgcgtc gacgagcgtc
     5281 gccgagcccg atgcgaagtg catcgcttcg aagttttctt cggcgagctt gtcgtgcatg
     5341 cgcgagacag cttcgtcgag gcccgcttcg cagatctggt tcagttgcgc ttcgtgcccc
     5401 acgcagtagc cccacacgca cttgttcgcg atcgtgtgcg ccagtgacgg gcagttgacc
     5461 gcggcgccga gcgcaccgcg caggtcgtag ccgtattgcg cgaccatctg ctcgttgatc
     5521 gcggtccaga tgtagtcgcc gtacggaagg ccgtacgtgt gatcaccgat cgcgagtgcg
     5581 ccgttcgacg acgtgcacgt cggcgactgc gagcgcatgt ccgacggcag gtcggcgagc
     5641 gagaacgtct tgtcgatgcc cgcaggcgtg aggtcgagcg tcgtgagctc gtgggtcgcc
     5701 gtgccgtcgc tttggatcgt cagctggcta tcgagcgcga actgcgtcag cgcggtctcg
     5761 gcgagcgacg ccgtggcgcc ggcgatttgc gtgatcggcg taccgttgat cgtgaccttt
     5821 gcgatctcgc cgttgatcca gccgatgagc ttgtcttcga cgtagtcggg aaggtcgtcg
     5881 cggatctctt ggacggcggg gacgccggcg tcgtcggcga gatcgaggag cgtgccgccc
     5941 gggttcgtcg agaagctgcg gagcgtgagc acgacgtctt cggccgattg cggcagcagc
     6001 gcttcgacgg tgaggtcgac gttcgagtgc acgctgtacg cacccgacgc gaccggtggc
     6061 ggcggcggag tgggatccga gggatccgac gggtcggacg gatcggacgg atccgatgga
     6121 tcggacggat cggccggatc cgatggatcg gatggatcgg ccggatcgga cggatccgat
     6181 ggatcggcgg tatcggtcgg atccgccgga ttgtcatcgc cggcgggggg cgagtgatcg
     6241 tcgccttggg gggtggcaac acacgcagga agcgaggcag cgaggaggca ggctaggacg
     6301 aatcgcatga ggctggttct gagcaaggtc cttgccgatg gtacggcctc tgccatgtcg
     6361 tggaaatcgc gatcgtgact gacgagttct gtgcggacga ctgacaccta catcggcatg
     6421 cgactggcac cttcatggga gcaggagcag aagcgtcgtt agccgcgaat ctccgcgatc
     6481 attccgtcga gcgtgagaag gccgaggccc agcgcagcgg acagttgcgt tgcgacctcg
     6541 cgctcctctt tcgagacgtc atcgtccgcg agcgcgatcg ccatgccgag catgagtgcg
     6601 gcttcggcgt gagcgtgctt gccggagagc aattcggcga cgcgcgcgat gcgtggttcg
     6661 aagccgcgct cttcgacgtc gtggctcgcg tccgtgagta gctcgtcgat cttgtcgcgg
     6721 tccacgccgc cgaggatctc gagccggtcg agtagcgcgt cgtactcctg ctcgctctgg
     6781 ttgccgtcgg cggctgcgac caggaacgcg caagtcaccg ccgcttcgag gaatgtctcg
     6841 tctttgaggc ggtccgtcag ctcgtgcttc gaccaccacg ttcgcttctc gtagctcgcc
     6901 attgcgcgtc gttataaatg agaagcggtc cactttctca ttgatcctcc gacgcccctg
     6961 cgattacggt tcagcccgct aaacggggga atgatgagga ccacgtttct gctagtcgca
     7021 tctctattga tcgcgtgtgg gcccggtgca cgtcacaacg gtgacgacga cgacggccct
     7081 gattccgcta cgtgccagaa cgcatgttcg gtggatggtc acgcaatcgt cgattgccac
     7141 aacaacgtgg tcacgcaatg cgagggcgcc gctgcgtgcg atcccgcaac atttacctgc
     7201 gccgacgcgt gcacggcggc cgaatcgaac catcgatccg tcggctgcga ttactacgcg
     7261 acggatatgg actcgctgtt cggcggctat tgctacgcgg tgttcgtcgc gaacacgtgg
     7321 acgtcgcccg cacatatcaa cgtcgactac aaaggccagc agctcaacgt tgcgacgttc
     7381 acgcggatcc cgagcggatc cggaccgacg ttgacgcaag gcacgttcga tccagcagct
     7441 ggtctcgcac cgggacaagt cgcgattctg ttcctcggcg gaacgcaagg cgccgcgccg
     7501 aactgccccg tcacgccggc cgcggctgcg ccatcgctca gcggcaccgg catctcggat
     7561 gcgttccaca tcaagacaga cgtccccgtc gtcgcgtatc agatcaaccc atatggcggc
     7621 ggctcggttg ccgtcactgc ggcatcgcta ctgcttccga cgagcgtgtg ggatcaagag
     7681 tacgtggcgg tgaacgtgtc ggcggcgtcg gcgggcaatc cgtcgatgaa catcgtcgcg
     7741 aaggaagacg gcacgaccgt cacgctcacg cccaatgcgg cgctcagcgg cgcgggcggg
     7801 attccgtcgg gggccgcggg cagccccgtg acgatcaatc tcaacaaagg tcagcacgcg
     7861 cagatcacgc aagcgaccga gctcaccggc agcaccgtgc agtcgaacaa gccggtcggc
     7921 ttcatggccg gtcacacgtg catgaatatg ccggtcggca cgtcgtactg cgatcacggc
     7981 gagcagatgg ttgcacccgt gcaagcgctc ggcagccgct acgtcggcgt gatgtatcgc
     8041 ccgcgcgtcg cgcaagagac cggcacgtac tggcgtgtga tcggcgccgt cgatggcacg
     8101 cagctcacgt actcgaccaa cgtcggcggt ccgacgacgc tcgccaaagg ccagcaggtc
     8161 ctgttccaga cgggcacgcc gttcaccgtc gagagccagg ataacgatca cccgttcatg
     8221 ctcttcacct atatgacgag ctcgaacgca gtctctgatg gatacggcga tcccgatttc
     8281 gtcacgagcg tgccgcccga gcagtatctg cagaactacg tgttcttcac cgacccgacg
     8341 tatccggaga cgaacatcgt catggttcgc gcgcgcagcc agatcgacaa caacttccac
     8401 gacgtcatgc tcgactgcgc gggaccgatc accggctggc agccgatcgg caacggcgag
     8461 tacgagtacg cccgcaagga tctcatgacc ggcaacttca cgtcggtcgg cacctgctcg
     8521 acgggccgcc acgagatcaa gagcgacgcg ccgttcggcc tgtgggtgtg gggctggggc
     8581 acgccgctca cgtcgacgtt caccgcgaac gtgtcgtacg gctacccagg cggcatgaac
     8641 gtgtcgccga tcaacagcgt cattctctag aagactgcgt agtcttcggt gagcttcgtg
     8701 tagccggtga tgcggatctc gtggatccgc gccgtcgggt cgtcctcgat ctgggcaatc
     8761 tcggcttcgc tgcgcatgaa ccacggatcg agcaggcagt cgtcttcgac ctcgatgtcg
     8821 acctcgagct cgggatcgcg acggcgtgtc acgggctcca gctcgcgaac caggtcgtag
     8881 agggcaggct ccgtcattca tcaatgcatc gcggagtttg cgatcggggg caactttgca
     8941 tccctcgggc gacagggtgc ggacgtacag ccgtcgctga ttccccactt agcgcgattc
     9001 gcgtcggtgg cacgaggcgt gatgaagacg gagtcatgga cctcacactc gacgagctcg
     9061 cgagcgtccg cggcggatac tccgatgccg acttcgggcg gtgcggtccg ggctcgcatc
     9121 tgaagttcct cggcgacgta cggacgagcg agtgcgcagc gcacgatgga gcggtccgcg
     9181 gtgcgttggc gaacgggagc tcgcaggtcg gtgcccagct ccgcgcgttg ccactgctgc
     9241 cggcggcggt tggctcgtat ttccgcgcgc gattcaccgg caagtaaggt ggtcggttaa
     9301 cgctggggat gcgagcacac gaagcctctg gtcggacctc atcgaaacct gtacatctcg
     9361 ccaatcgtgc cgaggcagtc agatcgttac gcgttggatt tggtggtcga gctctacgct
     9421 cagggtgagt ggcgcgccgt cgcgctgcag gacgtctcgc gaacaggcat gttcctcgcg
     9481 acgaacgcgc cgattgctgc ggggacgccg gtgatcgtcg cgttcaccga cgacgaagac
     9541 atgcgcgtcg cgtgtcaggc gcgcgtcacg cactttcttc ccgtcgccga cgcggagacg
     9601 ctcgggcgga ttccgggcgt aggcatcgtg ttccgcgagc cgcaggacgt ggcgttttca
     9661 gccgctgtcg aatcggtgct tcgtcgcgcg cgcacgcgcc agcccgagca cagccacatc
     9721 gtcgtcggcg ctgcggagca tcgcttgctc gaacgtctgt cgaccgcgct cggcggcgca
     9781 ggcttctcgg tcgccaccgc gacgaatggc ctcgagttct ggtcggcgtg tatgcgccgc
     9841 ttgcccgatg tcgtgctcat cgatcgcgaa atgccgatcc tcgacggtcc gacgatcatc
     9901 gagcgcctcg cgtgtgacga tcagctcgcg agcgtgccgt cgatcctgat gacgaaggac
     9961 gccggcgagc tcggtcccgc gctcgcgcgt ggtgctgccg atgtgatcct caagccgttc
    10021 acgctgctcg agctgatcgc gcgcgcacgt cgcgtggctc aatcaccgaa acgcacggag
    10081 cgcgtcgcac tctcgggctc gcttgccgat atcaacctcg gtgcgctgct cactctcctc
    10141 gagcaacagc gtcagacggg ccgcatcgtg ctgtcgaacg gccacgcggc atggatcgac
    10201 atcctcgacg gcaagatcgt cgacgccggc tggtcacgcg gcagcgctca cccgcgcgct
    10261 gtcatgatgg agctcctcga ctggagccac ggcacgttca agctgacgcc gcctccgacg
    10321 cggcgccggg acagcgatct acgcttgccg atcacgcacc tgctcctcga gcaagcgcgt
    10381 ctacgtgacg aagcgaatca ccgaaaagcg atcggctgaa cgacgacagc actctcgcgg
    10441 tcgctcgtcg ccgaaaacgt gacgccgttg agagccgtca tcacgcaggc ggcgaaaccc
    10501 tcgccccagc gatcgtcgac tcgaatctgc gtgatcttgc cggtcggctc gaccatcaag
    10561 gtcacgcgca cgcttccgga ctcaccgccg ccattgcgct cgaagcagcg ctgcaacatc
    10621 tcggcgcgcg ggcgaaacct gcgaccgacg cttcccgctt cgatcccccc ggcgattccg
    10681 ggcgtgccga acacggggga gccatctgat tctgcatcga cgatcgcatc acgcgcacga
    10741 gaggccgcga gcactgcagg cgactcctcc gcaatcggat ccgcttcgtc ggggacggcc
    10801 accgcaggct ctggcgcggg cacgggcacg gtgacggcgg cattcgcggg cttcttcgag
    10861 ccggctggag cggtccgcgt cgtcgcggcg tcgtcggact tctgccacat cgacttgttg
    10921 tcatgcatcc agcgatacac gccgtatcca gcgccgatcg agatcacgac cgagaacacc
    10981 agatacatcc cgatgccgcc gtgctgccga ttcacttcgg actccgggcc gcggccgacg
    11041 ccaaccactt ctgcgtgcaa tcgctcatgc tcgccatccc cttctccatc tccgccagaa
    11101 cggcgcgctc gatcttgccc gagagcagcg acacattcac gttgatcgtg cccttgtagc
    11161 gtcgtcgaac tctgccttcg ccgacctgcg tgaacgtata cagcgccgca atctgcacgc
    11221 ggcctccgcc gatctgcgga acgatcgtca tgtcgatctc gttgtcgcgc ttgcgccacg
    11281 tcatcgtctc gacgtacttc agcctgccac cctcgacgaa cggcttcacg agcgccggca
    11341 atggccgcag cgaccgcacg gtccagctgc agaacctcgt cgcctcatcc tcgcgcgact
    11401 cggtcaccgc tctctcgctg agctggccga cgacgtcttg agcagccagg tggtctggat
    11461 cgaagtacgc cgccccgatg gtctcggccg tcaatccagg tgcttcgaac acggtctcga
    11521 acgaatactc agtcgacatg ctggcaaccc ttttgcaccg tagcaaacgg atgcgttgcg
    11581 cggccttgtt ggtggctact ttagtagcct gcgcgagccc cggcgccgac gacgacctcg
    11641 gcatcgatga cccatctggc gaaggcggca aagacgataa cgggagcagc cggcgcttca
    11701 ccgaggtcga cgcgacccac accaaccaaa cgttccgcac gtacatcgaa cgcgccctcg
    11761 acgagctcgc tgccagcgac cgcgaagtcg cccgctacac cgcgcaatcg atcgccgacg
    11821 ggcgcgtccg catcgacgag ctctccgacc tgacgtgcgc ggacttcctc cacgtcatca
    11881 acgacctccc agacctccac ctcaaaccgt ccgactacgc ctcgctcaaa cgccgcggca
    11941 atccaaccac cgccgcgatc tccgccgagc tcgacggcta catgtggagc aaccgcatct
    12001 acgtcgcgcg cggcatgtcc gcacaacacg tcgccgctac gctcgtgcac gaaaccaatc
    12061 acgtcgtgaa tcgctccgaa gtcggctact acgacgacct gccgacgagc gcgttcatcc
    12121 acgagtaccg cgcgttctac gtcgaacgca ccttcgatcc ggactcctac gcgggcatcg
    12181 acctcgtcga ctacgtcatc acgaactacg agctcgaccg ctcgaagatc cgccccgaga
    12241 tcctcgccca cccactgacg cccaagctcc tcccgacgcc cgctgcatgg caagctcgcg
    12301 acgtctcatc cgacgtcccg gagcccgcca cgtgccaata aaagcgccat ccaagaccta
    12361 cagaacaacg atcttccgcg acggatcgat gtgctacatc ccggtcgcgt tcgatcccaa
    12421 acccgtcttc ggcaagattc gcgcgccggt caaagtcacc gtcaacggct tcacattcgc
    12481 gtcgacgatc tcgccgatgg gccacgacgg tccatgcatc ccattccgcc gctccaaccg
    12541 cgaagccgca ggcctcgaag gaggcgagac cctcgacgtg accgtcgcgc tcgacacgtc
    12601 cgaacgcaaa gtcaccgcac ccgctgatct cactcgcgct ctacgcgccg ccaagctcac
    12661 ggcccgctgg gccgagctct cctacacgca ccaaaaagag cacgtcgacg cgatcgaaaa
    12721 cgcgaaagcc cccgaaaccc gcgcccgccg gatcgcagcc gcgatcaaga tgctcgccgc
    12781 cgctactccg tctcgcaagc gccaaagccg gggcacttga agtcaaacga tccgctcatc
    12841 gtgacgttcc ccttcgcgcc cttgccgaca aacttcccgc tcagactgat cgtgccggcg
    12901 atgtgcttct tgtcgaacgc cgtcacatcg atggtcccat tcggatcgcc gagcgtcgcg
    12961 gtctgaccga gcgccggaaa caacacgttc acgctcgcgc catccgagtg cccgcccttg
    13021 aagacgtact tcttcggcgc aaacggcatg agctccggct tcccgatcgg cgtcatcgag
    13081 aacttgatgt ccttgcccga gcaattgacg gcaaagccat ccacgcccat caagttcttc
    13141 ttctcttcct cggtcatcca atacgtcacg ttcgtcgccg gccgcccgcc aaacgtcgtc
    13201 tgctccgcgg tcacagcgcc gctgaccttc agctcgcact tgcccatctc ctcgggcgtc
    13261 agcgctttct tgggcggcgg cggaggcggc ttcgtctcgg aaactgcagg cttatcctcc
    13321 tc
//
LOCUS       DATLBZ010000401         6722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1962699, whole genome shotgun sequence.
ACCESSION   DATLBZ010000401 DATLBZ010000000
VERSION     DATLBZ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962699"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2469
                     /locus_tag="VMZ53_20270"
     CDS             <1..2469
                     /locus_tag="VMZ53_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30863.1"
                     /translation="AAHIQSNSTMICGDAYSGAGSNPTAVYTGNGNIGGAKRAQTTNL
                     AMPAVTLPVLAAGSPFSPSSQNANTTLVANKSYGVVTCKNGSLTLSAGKYVVQKLILQ
                     ANCQLKMSSGPIEFYFSDTLDAQGGVFVNTTNVPSNLVFYGSSTATQVHLQGGVSAYF
                     AVYAPSAQCQLQGNVDMYGAVICDRVHVQGNAHVHYDLALRNFAGGGFACSAGETSRA
                     TPIVATIDNRGSIVQGTFVQPTGSPKTITTTADIATFAFPYITGHMRARTTSSISTSA
                     SGFSSGTVVFDAGATGKIPAANYAGCNTFTGACRNVFTVTQSPTSTGTSLRPPRVQLK
                     DSNASTIGALIAPASAVPGITAANWQTIVRKVLDAKLGGVDRSTVAVIQASPLAGNAT
                     RPTIAYFGATDGMLHAVCASTGGTTDSGTNICPSLGAELWAFMPRVQLPLVRKNTARI
                     DGSPRVVDAFGDFTGTGQRRFRTILTIQTGYADTSIGASPAVYALDVTDPANPSVLWE
                     YTRPTTLGTRELGVGLSLAAGPTLIGGARANLVVAETNNGGTGGAGVVATAIDLETGA
                     RKWQFSYLYPSPPRGDNTALPLPSTGIPGGAVGVDLDRFGYTTDIVMGDLFGNLWRLD
                     AATGTSRTGAVPLFQFSTNKHPIGVVPAIYGDTSSAQYAAFTTGGYADPTAVSWSAGT
                     QKLVAIDLKAAGPYPIAKTATSRLALARDLGSNERGFAQVLVVGNELFAITDSTDINS
                     STYGTSGATTGHVVSYNLTTAAATTVVVRGGAGGVANAGATLYASTSDKQQQLTTAAQ
                     GTTGVSVDTQAVAKLTRLLWLRSE"
     gene            complement(2470..3276)
                     /locus_tag="VMZ53_20275"
     CDS             complement(2470..3276)
                     /locus_tag="VMZ53_20275"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS30864.1"
                     /translation="MSCLVCHEPRELVGGACNECLDAIRPTIALTPEQVSLRSNGAAL
                     TSLLLDPWGRVRPLERRMIIGRVVDGPGLTILDTTISRRHAAIELQDNCWYVDDFKSA
                     NGTFVEGVRIQARTPLRDGERVRFGEVSFFFLDPVSAPPNIDTSSLQGFTVRGSVIED
                     QGANGIRIELREPSGGGGGIAVIDGKHVGLTLPQFELVALLHARAQSDRNAFVHVNEL
                     AKTLSLDSVEPNEDNVRQLVRRLRRLLFKAGINGLIESRYGLGYRLVLTR"
     gene            3355..3777
                     /locus_tag="VMZ53_20280"
     CDS             3355..3777
                     /locus_tag="VMZ53_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30865.1"
                     /translation="MELRVGRCDLSIMGPSPVPDDLVEAWQGTLSQWTNKLAHDTLLG
                     RAVKHNQLAWLATRYRESARANPADYIARDRLKGVQRAATMLAFASSAPTSEERRTAR
                     GGPALLVVAVLSTGLGLWLTDYVRVHRPHAAQVSPRSP"
     gene            3792..4778
                     /locus_tag="VMZ53_20285"
     CDS             3792..4778
                     /locus_tag="VMZ53_20285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HUS30866.1"
                     /translation="MLVERIVDSGLVPETVLRAGIRAVCALRLREQRNARTDALVDEL
                     RASDVAIETHAANAQHYEVPPAFFERVLGPHLKYSSCYWPDGVTSLGAAEEAMLALTA
                     ERAGLEDGQDVLDLGCGWGSFSLWAAARYPGSRFTAISNSRPQREHIEARAAKRGLTN
                     LTVRTADVRQLELAAHSFDRVVSIEMFEHMRNYAVLLRRIATWLRPTGSLFVHVFAHR
                     RYAYPFDDNGPSDWMAREFFTGGLMPSVGLFRHFQDDLVICDEWQLVGTHYAQTAEAW
                     HDNLLRQRAEIEALLGKRAYQRWRVFFLACAELFGYRNGSEWLVAHYRFAPR"
     gene            complement(4775..5635)
                     /locus_tag="VMZ53_20290"
     CDS             complement(4775..5635)
                     /locus_tag="VMZ53_20290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30867.1"
                     /translation="MTEQYRALKRLGVGAMGDVFCVENLAAQRFEAMKVLKKIDDPNI
                     REIAQARFKREVRAGHRLSHQNIVPTFDCGELPDGRLYLTMEFVDGPSLAQVLHHRGT
                     LPVPLVLGIIAEIADAIHHAHRAGVIHRDIKPHNLLLPHYTNGPMVKMLDLGLAKILF
                     GDFKESLVLSKDGMPFGTPMYMSPEQCQGKRSDARSDIYGLGCVAYELLTGSPPFTGN
                     IAMIFTGQLKKAPPPPSVVMPDARIPRQLDQIVLQCLQKSPELRFQNGAALCTAIQSV
                     PGYRPLRTAS"
     gene            complement(5667..>6722)
                     /locus_tag="VMZ53_20295"
     CDS             complement(5667..>6722)
                     /locus_tag="VMZ53_20295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30868.1"
                     /translation="RVENLEAIVCSVDYELNKKLAKVIDEHRSLVLAPGNPGPMQVAT
                     DAGAAKGDAKAADHPTPALDRTATAHGAVAKPLTTTLEPGQVLAQRYRVQRLLGKGGM
                     GAVYLADDEVLGELVALKVISSAFASDEQAMIARFRREAASARKVSSPSVIRIHDLGE
                     ARPGLLYLSMEYFAGRTLADVIAQRGLVPMKDVQDMLQQICSGLEAAHSAGVIHRDLK
                     PSNVLVGERGAVKIIDFGLATTQMGEGLTATGAILGTPHYMAPEQVRGKPVDARSDIY
                     ALGALTYHLVTGRPPFGGDNAIAIGFAHLSEAPTPPRQLRKDCPEALDAAILGALAKT
                     PDERPASAKAFFDKAFA"
BASE COUNT         1124 a         2279 c         2212 g         1107 t
ORIGIN      
        1 gccgctcaca tccagagcaa ctcgacgatg atctgcggcg acgcgtattc cggcgcgggc
       61 agtaacccga ccgcggtcta caccggcaac ggcaatatcg gcggagccaa gcgcgcccag
      121 acgacgaacc tcgcgatgcc cgccgtcacc ctgcccgttc tcgcagcggg ctcgccattc
      181 tctccgagct cgcagaatgc gaacacgacg ctcgtcgcga acaagtccta cggcgttgtc
      241 acgtgcaaga acggcagcct cacgctctcc gcgggtaagt acgtcgtgca gaagctgatc
      301 ctgcaggcga actgccagct gaagatgtcg tcggggccga tcgaattcta cttctccgac
      361 acgctcgacg cgcagggcgg cgtcttcgtg aacacgacga acgtgccgag caacctcgtg
      421 ttctacggca gctcgacggc aactcaggtg catctgcagg gtggcgtgtc cgcctacttc
      481 gcggtgtacg caccgagcgc gcagtgtcag ctgcagggca acgtcgatat gtacggcgcc
      541 gtgatctgcg atcgcgtgca tgtgcagggc aacgcgcatg tccactacga cctcgcgctt
      601 cgcaatttcg cgggcggcgg gtttgcgtgc agcgccggcg agacctcgcg cgcaacgccg
      661 atcgtcgcca ccatcgacaa tcgcgggtcc atcgtgcaag gcacgttcgt tcagccaacc
      721 ggaagcccca agacgatcac gaccaccgcg gacatcgcga cgttcgcgtt cccgtacatc
      781 acgggacaca tgcgcgcacg cacgacgtcg agcatctcga cgagcgcgag tggtttctcg
      841 tcgggaaccg tcgtgttcga tgccggagcc accggcaaga ttcccgccgc gaactacgcg
      901 ggctgcaaca cgttcacggg cgcgtgccgc aacgtcttca ccgtgacgca gtcgccgacc
      961 tcgacgggga catcgctcag gccgccgcgc gtgcagctca aagacagcaa cgcgagcacg
     1021 atcggcgcgc tgatcgcacc tgcgtctgcg gtgcccggca tcaccgccgc gaactggcag
     1081 acgatcgtgc gcaaggtcct cgacgcgaag ctcggcgggg tcgatcgctc gacggtggcg
     1141 gtgatccagg cgagcccgct cgcggggaac gcgacgcggc cgacgatcgc gtacttcggc
     1201 gcgaccgatg gaatgctgca cgcggtctgc gcgagcaccg gcggcacgac ggacagcggg
     1261 acgaacatct gcccgagcct cggtgccgag ctctgggcgt tcatgccgcg cgttcagctg
     1321 ccgctcgtgc gcaagaacac tgcgcgcatc gacggctcac cgcgcgtcgt cgacgcattc
     1381 ggcgacttca cgggcacggg ccagcgccgg ttccggacca ttctcacgat ccagacgggt
     1441 tatgccgata cgagcatcgg tgcgtcgccc gccgtctacg cgctcgacgt gacggatccc
     1501 gcaaacccga gcgtgctgtg ggagtacacg cgcccgacga cgctcggcac gcgcgagcta
     1561 ggtgtcggcc tctccctcgc ggcggggccc acgctcatcg gtggcgcgcg cgcgaacctc
     1621 gtcgtcgcgg agacgaacaa cggcggcacc ggtggtgcag gcgtcgtggc gaccgcgatc
     1681 gatctcgaga cgggcgctcg caaatggcag ttcagctatc tctatccgtc accgccgcgc
     1741 ggcgacaaca cggcgctgcc gctgccctcg acgggaattc ccggcggcgc tgtgggcgtc
     1801 gatctcgatc ggttcggcta cacgaccgac atcgtgatgg gcgacttgtt cggcaacttg
     1861 tggcgactcg atgcagcgac gggaacgagc cgcaccgggg ccgtgccact gttccagttc
     1921 tcgacgaaca agcatccgat cggcgtcgtg cccgcgatct acggcgacac gagctcggcg
     1981 caatacgctg cgttcacgac gggcggctac gccgatccaa cggcggtgag ctggagcgcc
     2041 ggcacccaga agctcgtcgc gatcgacctc aaagcggcgg gcccctaccc catcgccaag
     2101 acggccacat cgcggctcgc cctggctcgc gacctcggca gcaacgagcg cggcttcgcg
     2161 caagtactcg tcgtcggcaa cgagctgttc gcgatcaccg actcgaccga catcaattcg
     2221 tcgacgtacg gcacgagcgg tgccacgacg ggccacgtcg tctcgtacaa cctgacgacg
     2281 gcagctgcaa cgaccgtggt ggtgcgcggc ggcgcaggcg gagttgcgaa tgctggcgcg
     2341 actctctacg cgtcgacgag cgataagcag caacagctca ccaccgctgc gcaaggcacg
     2401 accggtgtca gcgtcgatac gcaggctgtc gcgaagctca cgcggctgtt gtggctgcgc
     2461 tccgagtgat cagcgcgtga gcacgagccg gtagccgagt ccgtagcgcg attcgatgag
     2521 gccattgatg ccggctttga acaggagccg gcgcagccgc cgcacgagct gacgcacgtt
     2581 gtcctcattc ggctcgacgg aatcgagcga cagcgtcttt gcgagctcgt tgacgtgcac
     2641 gaaggcattg cggtcgctct gcgcgcgcgc gtgcagaagt gcgacgagct cgaactgcgg
     2701 cagcgtcaag cctacgtgct tgccgtcgat cacggcgata ccgcccccgc caccgcttgg
     2761 ctcgcgcagc tcgatacgaa tgccgttggc accttggtct tcgatcaccg agccgcgcac
     2821 ggtgaagccc tgcagcgagc tggtgtcgat gttcggcggc gccgagaccg gatcgagaaa
     2881 gaagaagctg acctcgccga accgcacgcg ttcaccatct cgcagcggcg tccgcgcctg
     2941 gatgcgcacg ccctcgacga acgtgccatt ggcgctcttg aagtcgtcga cgtaccagca
     3001 gttgtcctgc agctcgatcg ccgcgtgacg gcgcgagatc gtcgtatcga ggatcgtcag
     3061 gccgggcccg tcgacgactc ggccgatgat catgcggcgc tccagcgggc ggacacggcc
     3121 ccacggatcg agcaagagcg acgtcagcgc ggcgccattg ctgcgcagcg agacctgctc
     3181 cggcgtcagc gcgatcgtcg gccggatcgc atcgagacac tcgttgcagg caccgcccac
     3241 gagctcgcgc ggctcgtgac agacaagaca gctcacgatt cgagtgtccc tcgaaacatt
     3301 cttccgcggc agagctgcta ccccggcgtt catgatccag ttacccgtga attcatggag
     3361 ctacgcgtgg gccggtgcga tctatcgatc atgggaccga gccccgttcc ggatgatctc
     3421 gtcgaagctt ggcaaggcac gttgtcgcaa tggacgaaca agcttgcgca cgacacgctc
     3481 ttgggccgcg cggtgaagca caaccagctc gcgtggctcg cgacgcgcta tcgcgaatct
     3541 gcgcgcgcca acccggcgga ctacatcgca cgcgatcgcc tcaagggcgt gcagcgcgct
     3601 gcgacgatgc tcgcgtttgc atcgtcggca ccgacgagcg aagagcgacg caccgcgcgt
     3661 ggtggccctg ccctgctcgt cgtcgccgtg ctgagcaccg gcctcggcct gtggctcacc
     3721 gactacgtgc gcgttcaccg gccgcacgcc gcgcaggtct cgcctcggtc gccgtgaggt
     3781 aagctcgcga cgtgctcgtc gagcgaatcg tcgattcggg gctcgtaccg gagacggtgc
     3841 tgcgcgcggg gattcgcgcg gtctgtgctc ttcggctacg cgagcagcgc aacgcacgaa
     3901 ccgatgcgct cgtcgacgag cttcgggcaa gcgacgtggc catcgagacg cacgcagcga
     3961 atgcgcagca ctacgaagtt ccgccggcgt tcttcgagcg cgtgctcggg ccgcacctca
     4021 agtactcgtc gtgctactgg cccgacggcg tgacgagcct cggcgccgcg gaagaagcga
     4081 tgctcgcgct cacggcggaa cgcgctggcc tcgaagacgg tcaagacgtc ctcgatctcg
     4141 gctgcggttg gggctcgttc tcgctgtggg ccgcggcgcg ctaccccggc agccgtttca
     4201 ccgcgatctc gaactcgcgg ccgcagcgag agcacatcga ggcgcgcgcg gcgaagcggg
     4261 ggctcacgaa cctgacggtc cggacggcgg acgtccgaca gctcgagctc gcggcgcaca
     4321 gcttcgatcg cgtcgtgtcg atcgagatgt tcgagcacat gcgcaactac gcggtgctgc
     4381 tccgccggat cgcgacgtgg ctccggccga ccggctcgct gttcgtccac gtgttcgcgc
     4441 atcgccggta tgcgtatccg ttcgacgaca acggcccgag cgactggatg gcacgcgagt
     4501 tcttcaccgg cggtctcatg ccgagcgtcg gcctgtttcg ccactttcag gacgatctcg
     4561 tgatctgcga tgagtggcag ctcgtgggca cgcactacgc gcagaccgcc gaggcgtggc
     4621 acgacaacct gcttcgccag cgcgcagaga tcgaagcgct cctcggcaag cgggcgtatc
     4681 agcggtggcg cgtgttcttc ctcgcgtgcg cggagctgtt cggttaccgc aacggaagcg
     4741 agtggctcgt cgcgcactat cgattcgcgc cgcgttaaga cgccgtgcgc agcggccgat
     4801 accctggcac ggactggatg gcagtacaca acgccgcgcc gttctggaat cgcagctccg
     4861 gcgacttctg caggcactgc aatacgatct gatccagctg ccgcggaatc ctcgcgtccg
     4921 gcatgaccac actcggcggc ggcggcgcct tcttgagctg gccggtgaag atcatcgcga
     4981 tgttgccggt gaacggcggc gagccggtga ggagctcgta cgcgacgcag ccaaggccat
     5041 agatgtccga gcgcgcgtcg gagcgtttgc cctggcactg ctcgggcgac atgtacatcg
     5101 gcgttccaaa cggcatgccg tccttcgaca gcacgaggct ctctttgaag tcgccgaaca
     5161 ggatcttcgc gagcccgagg tcgagcatct tcaccatcgg cccattcgta tagtgcggca
     5221 gcaggaggtt gtgcggcttg atatcgcggt ggatcacgcc agcgcgatgc gcgtggtgaa
     5281 tcgcatcggc gatctcggcg atgatgccga gtacgagcgg caccggcagc gtcccgcgat
     5341 ggtgcagcac ctgcgcgagc gacggaccgt cgacgaattc catcgtcagg tagagccggc
     5401 cgtcggggag ctcgccgcag tcgaacgtcg gcacgatgtt ctggtgggac aaccgatgcc
     5461 cggcgcggac ctcgcgcttg aacctcgctt gcgcgatctc gcgaatgttc gggtcgtcga
     5521 tcttcttgag gaccttcatc gcctcgaagc gctgcgcggc gaggttctcg acgcagaaca
     5581 cgtcacccat cgcgcccacg cccaggcgct tgagcgcccg gtattgctcg gtcatcgtcg
     5641 gcagcacccg tcccccgccc agtatcttac gcgaacgcct tatcgaaaaa tgctttcgcg
     5701 ctcgccggcc gctcgtcggg ggtcttcgcc agggcgccca ggatcgcggc gtcgagcgct
     5761 tctgggcagt ccttgcggag ctggcgcggt ggcgtcggcg cctcggagag gtgcgcgaag
     5821 ccgattgcga tcgcgttgtc accgccaaac ggcgggcggc ccgtcacgag gtgatacgtc
     5881 agcgcgccga gcgcgtagat atcgctgcgc gcgtcgacgg gtttgccgcg cacttgctcg
     5941 ggcgccatgt agtgcggcgt tccgaggatc gcgccggtcg cggtgagccc ctcgcccatc
     6001 tgcgtggtcg cgagcccgaa gtcgatgatc ttcaccgcgc cgcgctcgcc gacgagcacg
     6061 ttgctcggct tcagatcgcg atggatgacg ccggcggagt gcgccgcctc gagtccgctg
     6121 cagatctgct gcagcatgtc ctgcacgtcc ttcatcggga cgagcccacg ctgcgcgatc
     6181 acatccgcga gcgtgcgccc cgcgaagtat tccatcgaca aatacagcag cccgggcctc
     6241 gcctcgccga gatcatggat gcgaatgacc gacggcgagc tgaccttgcg cgccgatgca
     6301 gcctcgcggc ggaacctcgc gatcatcgcc tgctcgtccg acgcaaacgc gctcgagatg
     6361 accttcagcg caacgagctc accgaggacc tcgtcgtcgg cgagatagac ggcgcccatg
     6421 ccgcctttgc caaggaggcg ctgcacgcga tagcgctgcg cgagcacttg gcccggctcg
     6481 agcgtcgtcg tcagcggctt tgcaacggcg ccgtgtgcgg tcgcggtgcg atcgagcgcg
     6541 ggcgtcgggt ggtccgccgc ctttgcatcg cccttggccg cgcctgcatc cgtcgcgacc
     6601 tgcatcgggc ccggattgcc cggtgcgagc acgagcgagc ggtgctcgtc gatcactttc
     6661 gcgagcttct tgttcagctc gtaatcgaca gagcagacga tcgcttcgag attctcgacg
     6721 cg
//
LOCUS       DATLBZ010000402         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2711494, whole genome shotgun sequence.
ACCESSION   DATLBZ010000402 DATLBZ010000000
VERSION     DATLBZ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2711494"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VMZ53_20300"
     CDS             complement(<1..545)
                     /locus_tag="VMZ53_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30869.1"
                     /translation="MVRGLMTLVGIVLVAGCGKETIEVKPATGDDYKHAALVQAVDHY
                     VAAGRTPDAYVELAQTVSTLRPSMDRAVGKEAELKMIVLALPPMQSMQGKSLRAKLDT
                     LALTVWPTLLQPPIAADTPLGAYDSKAAELAPKPGEDPDQYIQRICGDKGPLARDCKT
                     TVPEMQGHLVEAIAIRRATERA"
     gene            complement(594..1574)
                     /locus_tag="VMZ53_20305"
     CDS             complement(594..1574)
                     /locus_tag="VMZ53_20305"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUS30870.1"
                     /translation="MRVPLVVVTGFLGAGKTTLVNRLLARRAERAATGKLGIIVNELG
                     EVGIDGALLGGESARQVELPGGCVCCVLGDELDKTLLDLVAKNPGMEAIVLETTGVAE
                     PLPIAWAVQRDPVRDAVRLAAVVTLVDAANFVASRPVTVAVDAQVAYADVLLVTKAHI
                     AGEAETRAATEAASGIAPKALVRVGSTDDHAEWLEQLLADPDLEHAATDFRDDHGSHD
                     HSDGHCREGDRHVHGVDSTWLFVEGAVDLEELEDQLAELPANYVRIKGIVRDAAGQWY
                     AIHRVGLRVSSERIVRKADDSLGENGRIVALGSDLASEKVAACLEAAYLK"
     gene            complement(1589..>2008)
                     /locus_tag="VMZ53_20310"
     CDS             complement(1589..>2008)
                     /locus_tag="VMZ53_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30871.1"
                     /translation="PMIRYVNGDLAVAGGDERCKCGRGLSKIREIQGRVTETMYDGQG
                     NAVGGLVFNILFSTIGHVARSFQVVQKPDASVIFKVVPFEGRDLPSKEESVLRAHAER
                     YLPGVPFSIELVDDIPLTSAGKRRVVVCEMQQPRRSA"
BASE COUNT          340 a          670 c          650 g          348 t
ORIGIN      
        1 gcgcgttcgg tcgcgcggcg aatcgcgatc gcctcgacga gatgaccctg catctcggga
       61 accgtcgtct tgcagtcgcg cgcgagcggg cctttgtcgc cgcagatgcg ctggatgtac
      121 tggtccggat cttcgcccgg cttgggcgcg agctcggcgg ctttcgagtc atacgcaccg
      181 agcggcgtgt ccgcggcgat cggcggctgc aggagcgttg gccacaccgt gagcgcgagg
      241 gtgtcgagct tcgcgcgcag cgacttgccc tgcatcgatt gcatcggcgg cagcgcgagc
      301 acgatcatct tcagctcggc ttctttgccg acggcgcgat ccatcgaggg gcgtagcgtc
      361 gagaccgtct gtgcgagctc gacgtaggca tccggcgtac ggcccgccgc gacgtagtga
      421 tcgacagcct ggacgagcgc ggcgtgcttg tagtcgtcac ccgtggcggg cttgacctcg
      481 atcgtctcct tgccgcagcc agcgacaagg acgattccga cgagggtcat gagaccacgc
      541 accatgatca gtacgagaga ggtagcaagg tggatgccca cgaagggccc acatcacttc
      601 aggtacgccg cttcgaggca ggccgccact ttctcagagg cgagatcgct gccaagtgcc
      661 acgattctcc cgttttctcc gagcgaatcg tctgctttcc ggacaatgcg ttcgctggaa
      721 acacgcagac ctacgcggtg aatcgcgtac cactggcccg ccgcgtcgcg aacgattcct
      781 ttaatcctca cgtagttggc ggggagctcg gccagctggt cctcgagctc ttccaaatcg
      841 acggcaccct cgacgaataa ccaggtgctg tcgaccccat gcacgtgtct gtcgccttcc
      901 cgacagtggc catccgagtg gtcatggctg ccatggtcgt ctcggaaatc tgtggcggca
      961 tgctcgagat ctgggtcggc gaggagctgc tcgagccact cggcgtggtc atcggtcgag
     1021 ccaactcgca cgagcgcctt tggcgcgatg cccgacgccg cctcggtcgc cgcacgcgtc
     1081 tcggcttcgc ctgcgatgtg cgccttggtc acgagcaaca cgtcggcgta tgcgacctgc
     1141 gcgtcgaccg ccacggtgac tggacgcgac gcgacaaagt tcgcggcatc gacgagtgta
     1201 acgacggcgg cgaggcgcac ggcatcacgc acaggatcgc gctgcacggc ccacgcgatc
     1261 ggcaatggct cggcaacgcc ggtcgtctcg agcacgatcg cttccatgcc cggattcttc
     1321 gcgacgagat cgagcagcgt cttgtcgagc tcgtcgccga gcacgcagca gacgcaaccg
     1381 ccgggcagct cgacctggcg cgccgattca ccgccgagca gcgcgccgtc gatgccgact
     1441 tcgccgagct cgttcacgat gatgccgagc tttcccgtag cggctcgttc agcacgccgc
     1501 gcgagcaacc ggttcacgag cgtggtcttg cccgcgccga gaaagccagt cacgacgacg
     1561 agcggaacgc gcacacgagc agcctaccct acgcggagcg gcgcggctgc tgcatctcgc
     1621 agacgaccac gcggcgcttg cctgcagacg tcagcgggat gtcatcgacg agctcgatcg
     1681 agaacggcac gcccggcaga taccgctcgg cgtgtgcgcg caagacgctc tcttctttcg
     1741 acggcaggtc gcggccctcg aacggcacga ccttgaagat cacgctcgcg tcgggtttct
     1801 ggacgacttg gaagctgcgc gcgacgtgac cgatcgtcga gaacaagatg ttgaacacga
     1861 ggccgccgac ggcattgcct tgcccgtcgt acatcgtctc ggtcacgcgg ccctggatct
     1921 cgcggatctt cgagaggccg cggccacact tgcagcgctc gtcaccgccg gcgacggcaa
     1981 ggtcgccgtt gacgtagcga atcatcgg
//
LOCUS       DATLBZ010000403         8293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5692462, whole genome shotgun sequence.
ACCESSION   DATLBZ010000403 DATLBZ010000000
VERSION     DATLBZ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5692462"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VMZ53_20315"
     CDS             <1..421
                     /locus_tag="VMZ53_20315"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HUS30872.1"
                     /translation="AEKAGKLIPKDFQGRTRVMQWCFAAVSTVAPTLTALDLLGIFDP
                     DKTAPRLHTETDKLADRWFQGVERRLEGREWIATDEFTAADLMMACIVRDVRKTDRLT
                     RFPRIKAYYDRCTARPAWQRTLGLYAERLGVNVDDIR"
     gene            453..1367
                     /locus_tag="VMZ53_20320"
     CDS             453..1367
                     /locus_tag="VMZ53_20320"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HUS30873.1"
                     /translation="MTTAAYPIGTAGKPWGAPEREQWRSLQRVQRSYAADVISVVDRL
                     RSRFDVTQYGELDYAPDKYPLFAIKTRNWQDDLPIVLITGGVHGYETSGVHGALQFIE
                     NHGSEYANRVNLLVLPCVSPWGYERIHRWNPFAVDPNRSFREPALAEEAAAVMRVIAP
                     LRGKILVHLDLHETTDTDETEFRPALAARDGKPSEPGEIPDGFYLVDDSENPQPEFQQ
                     AIIDAVAKVTHIAPADADNKIIGSPVVAPGVIRYAVKELGLCASVSQARYTTTTEVYP
                     DSPRATPAQCNDAQVAAVRAAIDFATRS"
     gene            1394..2341
                     /locus_tag="VMZ53_20325"
     CDS             1394..2341
                     /locus_tag="VMZ53_20325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUS30874.1"
                     /translation="MRALVVGTLGAALCSACGDSAPSPASQIDRARYETDLTAIAGTR
                     SPGTPHWQEVQDLCADRFASLGFEVERHAYATGVNVIGVRTGTKLPAERVVVSAHYDS
                     VPNCEGADDNGSGVAATLEAARVLSLKPHDRTLVVACWDEEERGLIGSKAYVARAKAA
                     GDAITLSLVFETIGYRNTAPMSQKSDPQLETIFPDAGNQIKANEYRGDFALVVNDEPA
                     AAAVVDMQAVAKDIGLPIVNLSLLAPLKTLAPLRRSDHAPFWDAEYPAIFVTDTADYR
                     NTHYHCYNSTVDALADIDFAFAVASTKVVVAAAAGALDR"
     gene            2458..4896
                     /locus_tag="VMZ53_20330"
     CDS             2458..4896
                     /locus_tag="VMZ53_20330"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUS30875.1"
                     /translation="MSIPSPPADEAARVASLQSFDILDTGPEAAFDELAEVAARVCET
                     PTGLVTLIDAKRQWFKARYGFKASETDRAIAFCAHTIMKREVLEIEDATRDPRFNSNP
                     LVTQDPHIRFYAGAPIVTTDGYALGSLAVIDYKPRKLTDGQRATLIALSHQVVAQLEL
                     RRHLADLERKTAAALDRRDRRMSALLETSLDAIIFMDHRGRIIQANPAAEQTFGFTRD
                     QAIGQDMADLIIPPRLREAHRRGLAARVESGTARIANRRVEMTAQRIDGRELPVELTI
                     TELGIEDPPVFAGSLRDVTDQREAESKLRQSIERFELAARATHDVIYDWDLRTNALWW
                     SETLAANYGHEPAKLSAGIESWLELVHPEDRQRIEKSISAEIERRGAYWTNEYRFRRG
                     DGSYAQVLDRGYITYDAGGQPARMIGALADMTERRRLEEQLIRSQKMEAIGHFASGIA
                     HDFNNLLTVIECNSFFLADGVSEERAGCVKDILYATERAAGLTRQLLLLGRKQRLREA
                     DVDLNTLVVDMMSMLERMLTARTRVVSSIAPSVPPIRADASMLEQVLLNLVINARDAM
                     EHGGTITISTAPITVTARGTRHGLLATPGLYSSLSVQDTGTGIAPETLPHIFEPFFTT
                     KEIGRGTGLGLSTVYHIIKQHRGWLDVTSEIGRGSTFVIALPAVTDTQPKEPAPEPTQ
                     DVPRGTERILLVEDEPVLRSRMAQLLRQYGYEVLDAASGPAALELWRKHGGRVDLVLT
                     DIVMPGGLDGRELARQLRRGRADLRVIYVSGFSPEHAGQGEELVDGVNFLEKPYAPQK
                     LLQLIRTQLDAHRA"
     gene            4971..5270
                     /locus_tag="VMZ53_20335"
     CDS             4971..5270
                     /locus_tag="VMZ53_20335"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HUS30876.1"
                     /translation="MSNESKRGEGAAEELGGKIKKNIGKIIGNEQMEAEGLVKEKKGE
                     AKQEAAKSAERTKGKVEEIVGAVKNRVGAVLDDEEMQAEGKAKELKGEARQKANE"
     gene            5343..6206
                     /locus_tag="VMZ53_20340"
     CDS             5343..6206
                     /locus_tag="VMZ53_20340"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HUS30877.1"
                     /translation="MRFAIAFAIALAGCNSFFDLDVTELRPDEVDTDGDHVADELDNC
                     ATVANEDQLDSDGDMRGDACDPCFQCAVCTVGPEHDEDGDHIMDGCDNCPTVPNASLA
                     NADGDDLGDACDPDSRMQRRLLFDGFGEIGSQWQATAAWRASNDAAETVEGPHPYGYR
                     LTTGHGLITGAAHWTLEVAFDVPATANQFDAIGANLIGPDSLSAFQCEILYDDGGWNL
                     ANGIPMPITVAPGLMTMKLSSKQMQSYVCRVANVEASRLPIADKYPFGLELYATRLTR
                     YTYVEVIEGLE"
     gene            6203..7234
                     /locus_tag="VMZ53_20345"
     CDS             6203..7234
                     /locus_tag="VMZ53_20345"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS30878.1"
                     /translation="MRIAVVFDSAFDWTPDQYAAEMAKEIANRKNAEPDMHYQVANSL
                     RKSGHDVLLLGIKDDPHAMSTQLGAWKPDLVFNATEAFLDNSNLDYLVPALLEAEGYR
                     YTGAPPLALLVTRNKAMSKKILAFHDIKVPGFKSFRVGESPDESTNNLRFPVIVKPNA
                     ADASEGISQASIVHDLKALADRVAWIHERFDQPAIAEEYIEGRELYATMLGNGDKVEV
                     LPIVEMVFDQAGSRPEDRIATQKSKWDLDYRKRRGIRNVFARKLAKVAHERLMKTCHA
                     AWRAFWFRDYARIDVRLTKDNEIWFIEANANPFLSYGHDSAESAHKAGLRYDAFIQRI
                     VDEAMSRPA"
     gene            complement(7231..>8293)
                     /locus_tag="VMZ53_20350"
     CDS             complement(7231..>8293)
                     /locus_tag="VMZ53_20350"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HUS30879.1"
                     /translation="GITRLNVAMGLVGMYLVRDDVEGALGLPSGADEIPMIITDATFA
                     PNGQLQYPATWQPNVIGDEILVNGVVWPYLNVPRGKVRFRIVDGSGSRSYMLMLSDSA
                     TFYQIGTDQGLLPAPVPVTHLMLMPGQRADVVIDFASYAAGTKLQLVNMDDPVPNIMQ
                     FVVTANPGHTAPLPTTLRAVTPIDPATSVVTRDLRLTQRTDACTGNSWTIDDRDWADI
                     TERPMLGTTEIWNFHNETGVAHPMHMHLVRFQVVGHPEMGWLDTVTVPAQETVSVIAT
                     FTDFAGKFPYHCHMLPHEDNMMMRQFEVVDPRGPDAGVDPPPPSKSGGCCDAGSQPTG
                     AFVGALGFAFVVLRRRRRS"
BASE COUNT         1638 a         2820 c         2541 g         1294 t
ORIGIN      
        1 cgccgagaaa gccggcaagc tgatcccgaa ggatttccaa gggcgcacgc gcgtcatgca
       61 gtggtgcttc gccgcggtca gcacggtcgc gcccacgctc accgcgctcg atctcctcgg
      121 catcttcgat cccgacaaga ctgcaccaag gctccacacc gaaaccgaca agctcgccga
      181 ccgctggttc caaggcgtcg agcgtcgcct cgagggccgc gaatggatcg cgaccgacga
      241 gttcaccgcc gcggatctca tgatggcgtg catcgtgcgc gacgtccgga agacggacag
      301 gctgacgcgc ttcccacgca tcaaggcgta ctacgaccgc tgcactgcac gtcccgcgtg
      361 gcagcgcacg ctcggcctct atgcagagcg cctcggcgta aacgtcgacg acatccgcta
      421 gccgctcgtc ccaacagcgg tagactcgcc gcgtgaccac cgccgcatat ccaatcggta
      481 ccgccggcaa accctggggc gccccggaac gcgaacagtg gcgttcgctc cagcgcgtgc
      541 agcgcagcta cgcagccgac gtcatctccg tcgtcgaccg tctccggtcg cgcttcgacg
      601 tcacgcaata cggcgagctt gactacgcgc ccgacaagta tccgttgttc gcgatcaaga
      661 cgcgcaactg gcaagacgat ctcccgatcg tcctcatcac cggcggcgtc cacggctacg
      721 agacgagcgg cgtccacggc gcgctgcagt tcatcgagaa ccacggaagc gaatacgcga
      781 accgcgtcaa cctgctcgtc ctgccgtgcg tgagcccctg gggctacgag cggattcatc
      841 gctggaaccc attcgcggtc gatcccaacc gctcgtttcg cgagccagcg ctcgccgaag
      901 aagcggcggc tgtgatgcga gtcatcgcgc cactgcgcgg caagatcctc gtgcacctcg
      961 atctccacga gacgacggac accgacgaaa ccgaattccg ccccgccctc gccgcgcgcg
     1021 acggcaagcc gagtgagccc ggcgaaattc ccgacggctt ctacctcgtc gacgacagcg
     1081 agaacccgca gcccgagttc cagcaggcga tcatcgatgc ggttgccaag gtcacgcaca
     1141 tcgcgcccgc cgacgccgac aacaagatca tcggctcgcc cgtcgtcgcg ccaggcgtca
     1201 tccgctacgc cgtgaaggag ctcggcctct gcgcgagcgt ctcgcaagcc cgctacacga
     1261 cgacgaccga ggtgtatccc gacagcccgc gcgcaacgcc ggcccaatgc aacgacgccc
     1321 aagtcgcagc ggtgcgagcc gcgatcgact tcgcgacgcg cagctagcct gaaacctgtt
     1381 acaaccgcgc gcgatgcgag ccctcgtcgt cggcactctc ggcgcagccc tttgcagcgc
     1441 gtgcggcgat tcggcgccat cgcccgcatc gcagatcgac cgagcgcgct acgaaaccga
     1501 cctcaccgcg atcgcgggaa cgcgcagccc gggcacgccg cactggcaag aggtccaaga
     1561 cctctgcgcc gatcgattcg cgtcgctcgg cttcgaggtc gaacgccacg cctacgccac
     1621 cggcgtcaac gtcatcggcg tccgcacggg cacgaagctc cccgccgagc gcgtcgtcgt
     1681 gtccgctcac tacgacagcg ttcccaactg cgaaggcgcc gatgacaacg gcagcggagt
     1741 tgccgcgacc ctcgaggctg cacgcgtgct gtcgctgaag ccgcacgatc gaacgctcgt
     1801 cgtcgcatgc tgggacgaag aagagcgtgg cctcatcggc tcgaaggcgt acgtggcgcg
     1861 cgcgaaggcg gcgggcgatg cgatcaccct ctcgctcgta ttcgagacca tcggctaccg
     1921 caacaccgca ccgatgtcgc aaaagagcga tccgcagctc gagacgatct tccccgacgc
     1981 gggcaatcag atcaaagcga acgagtatcg cggcgacttc gctctcgtcg tcaacgatga
     2041 gcccgccgcg gccgctgtcg tcgacatgca agccgtcgcg aaggacatcg gcctgccgat
     2101 cgtgaacctc tcgctgctcg cgccactcaa gacgctcgct ccactacgcc gctcggatca
     2161 cgcgccgttc tgggacgccg agtacccggc catcttcgtg accgacaccg cggattaccg
     2221 gaacacgcac taccattgct acaactcgac ggtcgacgcg ctcgcagata tcgacttcgc
     2281 cttcgccgtc gccagcacca aagtcgtcgt cgcagcggcc gccggcgcgc tcgatcgcta
     2341 gtacgcgctc actaacgttt gaacccagtg attcctggat ttcagtgacg cggttttttt
     2401 ccgcgcacgt cagggagagg cagacggcgc gacgaagagc gtagattacg ggcgtccgtg
     2461 tctatcccgt caccgccggc cgacgaagca gcgcgcgtcg cgtcactcca gagctttgac
     2521 attctcgata ccgggcccga ggccgcattc gatgagctcg cggaagtggc tgcgcgcgtg
     2581 tgcgaaacgc cgacgggtct cgtcacgctg atcgatgcga agcggcagtg gttcaaagct
     2641 cgctatggct tcaaggctag tgagacagac cgcgcgatcg cgttttgcgc gcacacgatc
     2701 atgaagcgcg aggtcctcga gatcgaggac gccacccgcg atccgcgctt caacagcaac
     2761 ccgctcgtga cgcaggaccc gcacattcgc ttctacgcag gcgcgccgat cgtgaccacc
     2821 gatggttacg ccctcggatc gctcgccgtg atcgactaca agccgcgcaa gctcaccgac
     2881 ggccagcgcg cgacactgat cgcgctctcg caccaagtcg tcgcgcagct cgagctccga
     2941 cgtcacctcg ccgatctcga gcgaaaaact gccgcggcgc tcgaccgacg cgaccgccgc
     3001 atgtcggctc tcctcgagac atcgctcgat gcgatcatct tcatggatca ccgggggcgc
     3061 atcattcagg ccaatcccgc ggccgagcag acgttcggtt tcacgcgcga ccaggcaatc
     3121 ggccaggaca tggcggacct catcatcccg ccgcgtcttc gcgaggctca tcgtcgaggg
     3181 ctggccgcgc gcgtcgaatc gggaacggcc cgcattgcga accgccgcgt cgaaatgacg
     3241 gcacagcgca tcgatggccg cgagctccct gtcgagctga cgatcaccga gctcggaatc
     3301 gaagatccgc cggtcttcgc cggatccttg cgcgacgtca ccgaccagcg agaagccgag
     3361 tcgaagctgc ggcagagcat cgagcggttc gagctcgcgg cgcgagcgac gcatgacgtc
     3421 atctacgatt gggatctgcg cacgaacgcg ctgtggtgga gtgaaacgtt ggcggccaac
     3481 tacggccacg agccagcgaa gctctcggcg ggaatcgagt cgtggctcga gctcgtgcat
     3541 ccagaggatc gccaacgcat cgagaagagc atcagcgccg agatcgagcg gcgcggcgcg
     3601 tactggacga acgagtatcg ctttcgtcgc ggcgacggga gctacgcgca ggtcctcgac
     3661 cgcgggtaca tcacctacga cgccggcggc cagcctgctc gcatgattgg cgcgcttgcc
     3721 gacatgaccg agcgtcgccg cctcgaagag cagctcattc ggtcccagaa aatggaagcg
     3781 attggccact tcgcgagtgg catcgcacac gacttcaaca acctgctgac cgtgatcgag
     3841 tgcaactcgt ttttcctcgc ggacggcgta tcggaggaac gcgcgggatg cgtaaaggac
     3901 atcctctacg cgaccgaacg cgcggcaggc ttgacgcgcc agctcctgct actgggccgc
     3961 aagcagcgac tgcgcgaggc agacgtcgat ctcaacacgc tcgtcgtcga catgatgagc
     4021 atgctcgagc gcatgctgac cgcgcgaacc agagtcgtct cctcgatcgc gccctcggtg
     4081 ccgccgatcc gcgcagatgc atcgatgctc gagcaagtgc tgctgaacct cgtgatcaac
     4141 gcgcgtgatg cgatggagca cggcggcact atcacgatct cgacagcccc gatcacggtg
     4201 accgcgcgcg gcacgcgcca tggtctcctc gcgacgccgg gtctctatag ctctctctcg
     4261 gttcaggaca cgggcaccgg catcgcgccg gagacgctcc ctcacatctt cgagccgttc
     4321 ttcacgacga aggagatcgg tcgcggcacg gggcttggcc tctcgaccgt ctatcacatc
     4381 atcaagcagc accgcggctg gctcgatgtc acctccgaga tcgggcgcgg ctcgacattt
     4441 gtcatcgcgc ttccggctgt gacggacacg cagcctaaag agcccgcccc cgagcccacc
     4501 caagacgtac cgcgcggcac ggagaggatc ctgctcgtcg aagacgagcc cgtgttgcgc
     4561 agtcgcatgg cgcagctact tcggcagtac ggctacgagg tgctcgacgc ggcatcgggc
     4621 cccgcggcgc tcgagctctg gcgcaagcac ggcggtcgcg tcgatctcgt gctgacggac
     4681 atcgttatgc cgggcgggct cgacgggcgc gagctcgcac gacagctacg gcgtggccgc
     4741 gcagacctcc gcgtcatcta cgtgagcggg ttctctccgg aacacgcagg acaaggcgaa
     4801 gagctggtcg acggcgtgaa cttcctcgag aaaccgtatg cgccgcaaaa gctacttcag
     4861 ctcattcgca cgcagctcga cgcgcaccgt gcgtgatcag caagtcgcgt aggaaacgcg
     4921 ctgggccgcc ccggcctgca ttttgctcct tttgtgtaca aggaggaact atgagcaacg
     4981 aaagcaaacg tggagaaggc gccgccgaag agctgggcgg caagatcaag aaaaacatcg
     5041 gcaagatcat cggcaacgag cagatggaag ccgagggcct ggtgaaggag aagaagggcg
     5101 aggcgaagca agaggctgcc aagtccgctg agcgcacgaa gggcaaagtc gaagagatcg
     5161 ttggcgcggt gaagaatcgc gtgggcgcgg tgctcgacga cgaagagatg caggcggaag
     5221 gcaaagccaa agagctcaag ggcgaggcac gccaaaaagc aaacgagtag ctgcgctgcg
     5281 aaagcgcaca actcgcgtcg cacattgccg cgatcgtgaa ccgcctgcga taggcttcgg
     5341 ccatgcggtt cgcgatcgct ttcgctattg cgctcgcagg ctgtaactcg ttcttcgacc
     5401 tcgatgtaac ggagctgcgt cctgacgaag tcgacacgga cggtgatcac gtcgccgacg
     5461 agctcgacaa ctgcgccacc gtcgcgaacg aagaccagct cgacagcgac ggcgacatgc
     5521 gcggcgatgc ctgcgatccg tgctttcaat gcgcggtgtg cacggtcggc cccgagcacg
     5581 acgaagacgg cgatcacatc atggacggct gcgacaattg cccgaccgtg ccgaacgcca
     5641 gcctcgcgaa cgcggacggt gacgacctcg gtgacgcgtg cgatcccgat tcacgcatgc
     5701 agcgccgcct gctgttcgac ggcttcggcg agatcggctc gcagtggcag gccaccgcag
     5761 cgtggcgcgc ttccaacgac gcggccgaga cggtcgaggg cccgcaccca tatggctacc
     5821 ggctcacgac gggccacggc ctgatcaccg gtgccgcaca ctggacgctc gaagtcgcat
     5881 tcgacgtgcc tgcaaccgcg aatcagttcg atgcgatcgg cgcgaacctc atcggtcccg
     5941 acagcctctc cgcattccaa tgcgagatcc tctacgacga cggcggctgg aacctagcga
     6001 atgggattcc aatgcccatc accgtcgcgc ccggcctcat gacgatgaaa ctgagctcca
     6061 aacaaatgca gagctacgtc tgccgcgtcg caaacgtcga agcgagccgg ctgccgatcg
     6121 ccgacaaata tccgttcggc ctcgagctct acgcgacgcg tctaacgcgc tacacatatg
     6181 tcgaagtgat cgagggactc gaatgagaat cgcggttgtc ttcgattccg cgttcgactg
     6241 gacgccggat cagtacgccg ccgagatggc gaaggagatc gcgaatcgca agaacgccga
     6301 acccgatatg cactatcagg tcgcgaactc gctgcgaaag agcggccacg acgtcctgct
     6361 cctcggcatc aaagacgatc cgcatgcgat gagcacgcag ctcggcgcgt ggaagccaga
     6421 cctcgtcttc aatgcgacgg aggcgttcct cgacaactcg aacctcgact atctcgtccc
     6481 tgccctgctc gaggccgagg gctatcgcta caccggcgcg ccgccgctcg cgctcctcgt
     6541 tacgcgcaac aaggcgatga gcaaaaagat cctcgccttt cacgacatca aggtgcccgg
     6601 cttcaagtcg ttccgcgtcg gcgaatcccc cgacgagagc acgaacaacc tgcgcttccc
     6661 ggtgatcgtc aagccaaacg cggcggacgc ctcggagggc atctcgcaag cgtcgatcgt
     6721 ccacgatttg aaggcgctcg cggatcgcgt cgcgtggatc cacgaacgct tcgatcagcc
     6781 cgcgatcgcc gaggaataca ttgaaggccg cgagctctac gcgacgatgc tcggcaacgg
     6841 cgacaaggtc gaagtcctcc cgatcgtcga gatggtcttc gatcaagcgg ggtcacgccc
     6901 ggaagaccgc atcgcgacgc aaaagtcgaa gtgggacctc gactaccgca agcgtcgcgg
     6961 cattcgaaac gtgttcgcac gcaagctcgc aaaggtcgcg cacgaacggc tgatgaaaac
     7021 ctgccacgcc gcatggcgcg cgttctggtt tcgcgactac gcgcgcatcg acgtccggct
     7081 cacgaaagac aacgagatct ggttcatcga ggcgaacgcg aacccattcc tcagctacgg
     7141 ccacgactcc gccgagtccg cgcacaaggc gggcctgcgt tacgacgcat tcatccagcg
     7201 catcgtcgac gaagcgatgt cgcgtcccgc ttagctgcgg cggcgacgac gaagcacgac
     7261 gaacgcgaac ccgagcgcgc cgacgaatgc gcccgtgggc tggctgcccg cgtcgcaaca
     7321 accaccgctc ttgctcgggg gcggcggatc cacaccggcg tccggcccgc gtgggtccac
     7381 gacctcgaac tgccgcatca tcatgttgtc ctcgtgcggc agcatgtggc agtggtacgg
     7441 aaacttcccc gcgaagtcgg tgaacgtggc gatcacactc acggtctctt gcgccggaac
     7501 cgtgacggta tcgagccatc ccatctcggg atgaccgacg acctgaaacc gcacgaggtg
     7561 catgtgcatt ggatgcgcga cgccggtctc gttgtggaag ttccagatct ccgtcgtgcc
     7621 gagcatcggc cgctcggtga tgtcggccca atcgcgatcg tcgatcgtcc acgagttgcc
     7681 cgtgcaggca tcggtgcgtt gcgtgagccg caggtcgcgc gtgacaacgg aggtcgccgg
     7741 atcgatcggg gtcaccgcgc gcagcgtcgt cggcaacggc gccgtatgcc ccggattcgc
     7801 cgtcacgacg aactgcatga tgttcggaac cgggtcgtcc atgttgacga gctggagctt
     7861 cgtgcccgcg gcatacgacg cgaagtcgat gacgacgtcc gcgcgttgtc ccggcatcaa
     7921 catgagatgc gtaaccggca ccggcgcagg caagagaccc tggtccgtgc cgatttgata
     7981 gaacgtcgcg ctgtccgaca gcatcagcat gtacgaacgc gagcccgagc cgtcgacgat
     8041 gcgaaagcgc accttgccgc gcggcacatt caagtacggc cacacgacgc cgttcaccag
     8101 aatctcgtcg ccgatgacgt tcggctgcca cgtcgccgga tactgcagct gaccattcgg
     8161 cgcgaacgtc gcatccgtga tgatcatcgg aatctcgtcg gcgcccgacg gcaacccgag
     8221 cgcaccctcg acgtcgtcgc gcaccaagta catgccgacg aggcccatcg cgacgttgag
     8281 ccgcgtgatg ccc
//
LOCUS       DATLBZ010000404         4727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1967438, whole genome shotgun sequence.
ACCESSION   DATLBZ010000404 DATLBZ010000000
VERSION     DATLBZ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967438"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..820
                     /gene="upp"
                     /locus_tag="VMZ53_20355"
     CDS             188..820
                     /gene="upp"
                     /locus_tag="VMZ53_20355"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HUS30880.1"
                     /translation="MPGKVHVIAHPLVQHKLTLMRSKETSTSTFRNLLREISMLLAYE
                     VCRDMPTTKVAIETPLAKMQAPMLDGKKIVLISILRAGNGILDGMLEILPSARVGHIG
                     LYRDPKTLNAVEYFYKVPDNMGDRDAIAVDPMLATGNSAVAAIDRLKESGPRSIKFVC
                     LLTCPEGIRVFHSQHPDVPVYTAAIDDALNEKSYIVPGLGDAGDRLFGTK"
     gene            922..2139
                     /locus_tag="VMZ53_20360"
     CDS             922..2139
                     /locus_tag="VMZ53_20360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HUS30881.1"
                     /translation="MIDGDTAWLLISAALVMLMTPGLALFYGGMVQGKNVLSTFMHSF
                     FALGLVTIQFALVGYSLAFGTSWHGIIGGFDYVGLDGLTETTARGNVPHLAFFAYQCM
                     FAVISPALISGAYAERFKFSAYVLFTLGWTTLVYDPIAHWSWGSNGWLAKLGAIDYAG
                     GTVVHLSSGLSALVVALVIGKRKGYPANRHPPHDLTMTVLGAGILWFGWFGFNAGGAG
                     ANGTAALALVNTHVAAAAGALTWVVIEGSRIKKFTILGFASGLVAGLVAITPAAGYVS
                     PMWAIAIGCAAGAICYGAVLAKARLGYDDALDAFGVHGVGGATGALLTGVAARAVLNN
                     GNGGGLSLLGKQALSIAAAGAWAAVVTFILLKVIDLTVGLRVDQESEHEGLDGVLHGE
                     SAYGSPGGIAHGS"
     gene            2400..3071
                     /locus_tag="VMZ53_20365"
     CDS             2400..3071
                     /locus_tag="VMZ53_20365"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS30882.1"
                     /translation="MASQRADKPRVDESPPEAHAAQAAPAPTSNGTNPEQTDLAPVVG
                     ANLRRLRTRRGLSLERLAQISGVSRAMLGQIELGQSAPTINVLWKIARALEVTFSALI
                     SARTQSGALVLRSSESKILTSKDRSFSSRALFPFDEPRRVEFYELRLSGGAVEDADAH
                     PPGTSENLVVTAGTVEIDVAGDTHKLDAGDSILFEADTPHAYRNVGKVEAVMYLVMTY
                     AEEIG"
     gene            complement(3068..3964)
                     /locus_tag="VMZ53_20370"
     CDS             complement(3068..3964)
                     /locus_tag="VMZ53_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30883.1"
                     /translation="MRLIVAVLACMIAVPTIADACQCDSSASLAIGPAPGTRLPQTPT
                     IYVFAPNALHDGTPFVHDIYVTNTATSRPNTTIRTRQIAIDPEYMVFEIESDASEGVL
                     ELHLTYGTPEVFRFPLSDNPPENRVRPLEMVVQEMTDCGMSNEKAVTLTLQGNAAAYA
                     LEWDDGRKTVLPAIDNWNSPTHDIRIGTRCSLTNVPFDRLHEEHRVTLRALFPNGTSR
                     IVATTTLQLSPTHVRTPIEILDARGGGTADFPSTAPRDIVIQATANPTGSSIAGGAAT
                     GGAVVIGGALLRARRRRHQNRL"
     gene            complement(4025..>4727)
                     /locus_tag="VMZ53_20375"
     CDS             complement(4025..>4727)
                     /locus_tag="VMZ53_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30884.1"
                     /translation="LFAWAQSERKQEHCDKALELYSKLLAMDMPAENKDAIKVQVGEC
                     KDILDKEKAAKPDKPDKPDKPDKPDKPDKPDKPDKPDTAQDTTEPHVAPDASHETTAD
                     APADTATTSSFQSPPSETRPSTPEGRAWWKDPVGGALVGAGVVGVGVGAVFLVQGSAA
                     ESDKVTAANYDDYVALDERAKSRGQLGMITIVAGGALTVGGIAWYATHKASHNATVTG
                     WLDGQSGGLVVRGGF"
BASE COUNT          764 a         1570 c         1510 g          883 t
ORIGIN      
        1 gaaagcaacc tccaaccgcc tctcgacgat cgccgcccgc aactcgaacc gcgtccgcga
       61 cgcattcttc gccgcgtgcg tcgctctcgt cgcgacggtc tcgttcgtca ccgtgacgac
      121 gacggcgcag gtcgcgcacg cgcagcccgc gtctcatgtc tcgcgctgag gtaacgttgc
      181 cgagctaatg cccggcaagg ttcacgtcat cgctcaccct ctcgttcagc acaagctgac
      241 gctgatgcgc tcgaaagaga ccagcaccag cacgtttcga aatctgctcc gcgagatctc
      301 gatgctcctg gcctacgaag tgtgccggga catgccgacg acgaaggtcg cgatcgagac
      361 gccgctcgcg aagatgcaag cgccgatgct cgacggcaag aagatcgtgc tcatctcgat
      421 cttgcgcgcc ggcaacggaa ttctcgacgg catgctcgag attttgccga gcgcacgcgt
      481 cggccacatc ggcctctatc gcgatccgaa gacgctcaac gcggtcgagt acttctacaa
      541 ggtgcccgac aacatgggcg accgtgatgc gatcgctgtg gacccgatgc tcgcgaccgg
      601 caactccgcg gtcgccgcga tcgatcgcct caaagagagc gggccgcgct cgatcaagtt
      661 tgtctgcctg ctgacctgcc ccgagggcat ccgcgtgttt cacagccagc accccgacgt
      721 gccggtctac accgccgcga tcgatgacgc gctcaacgag aagagctaca tcgttccggg
      781 cctcggcgac gccggcgacc gcctgttcgg caccaagtag cggcgtcctc gccgaaagct
      841 caccgagcga agcgggctcg gaaaacagct tttgcgcggc ggttacgcgc cggaaacata
      901 ctcccggtca aagtctttcg catgatcgac ggcgatacgg cctggctcct catttcagcg
      961 gcgctggtca tgctcatgac cccggggctc gcactgtttt acggcggcat ggtgcaaggc
     1021 aagaacgtgc tctcgacgtt catgcacagc ttcttcgcgc tcgggctcgt gaccattcag
     1081 ttcgcgctcg ttggttactc gctcgcgttc ggcacgagct ggcacggcat catcggcggc
     1141 ttcgactacg tcggcctcga tgggctcacc gagacgacgg ctcgcggcaa cgtcccgcac
     1201 ctcgcgttct tcgcgtacca gtgcatgttc gcggtgatct cgcccgcgct gatctcgggc
     1261 gcgtatgcgg agcgcttcaa gttctcggcg tacgtgctgt tcacgctcgg ctggacgacg
     1321 ctcgtctacg atccgatcgc gcactggagc tggggaagca acggctggct cgcgaagctc
     1381 ggcgcgatcg attacgcggg cggcaccgtc gtgcatctct cgagcggttt gtcggcactt
     1441 gtcgtcgcgc tcgtcatcgg caagcgcaag ggctatccgg cgaatcgtca tccgccgcat
     1501 gacctgacga tgacggtcct cggcgcgggc atcctgtggt tcggctggtt cggcttcaac
     1561 gcaggtggcg caggcgcgaa cggcacggcg gcactcgcgc tcgtcaacac gcacgtcgct
     1621 gcagcggccg gcgcgctgac gtgggtggtc atcgaaggct cgcgcatcaa gaagttcacg
     1681 attctcggat tcgcgtcggg tctcgtcgcc gggctcgtcg cgatcacgcc cgctgctggc
     1741 tacgtctcac cgatgtgggc gatcgcgatc ggctgcgcag caggcgcgat ctgttacggc
     1801 gcggtcctcg cgaaggcgcg ccttggttac gacgacgcgc tcgatgcgtt tggcgtccac
     1861 ggtgtgggcg gcgcgaccgg cgcgttgctg accggcgttg cggcgcgcgc ggtgctcaac
     1921 aacggcaacg gcggcggtct ctcgctactc ggcaagcaag cgctcagcat cgctgcggca
     1981 ggcgcgtggg ccgcagtcgt gacgttcatt ctgctcaagg tgatcgacct gaccgttggc
     2041 ctgcgagtcg accaagagtc ggagcacgag gggctcgatg gcgtcctcca cggcgagtcg
     2101 gcctacggga gtccgggcgg gattgcccac ggaagctgag caatttgtac cggttggcag
     2161 tttatccgta aaaacagatt gcgcgtttcc gctaaagcgg atacaaatct gtctatgagc
     2221 aacgctgccc ttgcccagaa catcgtgagc ctcgacgagt accgtcgtac gcgcggttct
     2281 cggacgacga tggcgccgcg tcccgccgct caaaccgccc cgacctatcc ggctgctgcc
     2341 gtgtgggtat actgggtgcc ggtttgggtc tggtgaagaa gaaagcgtca ggaaacaaag
     2401 tggctagcca gcgggcagac aagccgcgcg tcgacgagtc tccgccggag gcacatgctg
     2461 cgcaggctgc gccagctccg acttccaacg gcaccaatcc cgagcagacg gatctcgcgc
     2521 ccgttgtcgg cgccaacctc cgccgtcttc gcacgcgtcg tggcctgtcg ctcgagcgcc
     2581 tcgcgcaaat ctcgggcgtt tcgcgcgcga tgctcggcca aatcgagctt ggccagagcg
     2641 caccgacgat caacgtcctc tggaagatcg cgcgcgccct cgaggtgacg ttctcggcgc
     2701 tgatcagcgc acgcacgcag tcaggcgcac tcgtgcttcg ctcctcggag tcgaagatcc
     2761 tcacgagcaa agaccgcagc ttctcgtcgc gcgcgctgtt cccgttcgac gagccacgcc
     2821 gcgtcgagtt ttacgagctc cgtctctccg gtggcgccgt cgaagatgcc gatgcgcatc
     2881 cgcccggcac gagcgagaac ctcgtcgtca ccgcgggcac cgtcgagatc gacgtcgcag
     2941 gcgacacgca caagctcgac gcgggcgatt cgattctgtt cgaggccgac acgccgcacg
     3001 cctaccgcaa cgtgggcaag gtcgaagcgg tgatgtacct cgtgatgacg tacgccgaag
     3061 aaatcggtta gagccgattc tggtgacgtc gacgacgcgc gcgcagtagt gcgccgccga
     3121 tgacgaccgc gccaccagtt gcagcaccgc cggcgatcga cgatccggtc gggttcgccg
     3181 tcgcctgaat gacgatgtct cgcggcgccg tcgacgggaa gtccgccgtg cctcctcctc
     3241 gtgcgtcgag gatctcgatc ggcgtacgca cgtgcgtcgg gctgagctgc agcgtcgtcg
     3301 tggcgacgat gcgcgacgtc ccgttcggga acagcgcgcg caaggtcacg cggtgctctt
     3361 cgtgcagccg gtcgaacggc acgttcgtga gtgagcaccg cgtgccgatg cgaatgtcgt
     3421 gtgtcggcga attccagttg tcgatcgctg gcaggactgt cttgcggccg tcgtcccact
     3481 cgagcgcgta cgccgcggca ttcccctgca gcgtgagcgt gacggccttc tcgttgctca
     3541 ttccgcagtc ggtcatctct tgcacgacca tctcgagcgg gcgtacgcgg ttctcgggtg
     3601 gattgtccga gagtggaaag cgaaacacct cgggcgtgcc gtacgtgagg tgcagctcga
     3661 gcacgccctc cgatgcatcg ctctcgatct cgaacaccat gtactcggga tcgatcgcga
     3721 tttgtcgcgt gcgaatcgtc gtgttcggcc ggctcgttgc cgtgttcgtc acgtagatgt
     3781 cgtgcacgaa cggcgtgccg tcgtgcagcg cgtttggcgc gaacacgtag atcgtcggtg
     3841 tctgcggcag cctcgttccc ggcgcgggac cgatcgccaa cgacgcagag ctgtcgcact
     3901 ggcaggcatc cgcgatcgtc gggactgcaa tcatgcacgc gagcacggcg acgatgagcc
     3961 tcatcccggc gagacagcgc gagacgatcg cagttgcgcg cgagcgaagc gagccggggc
     4021 cgcgtcagaa gccgccgcga acgacgagcc caccgctctg accgtcgagc caaccggtga
     4081 ctgtggcgtt atgcgacgcc ttgtgtgtcg cgtaccaagc aattccaccg acggtgagcg
     4141 caccacccgc gacaatcgtg atcatgccga gctggccgcg cgacttcgcg cgttcatcga
     4201 gcgcgacgta gtcgtcgtaa ttcgcggcgg tcaccttgtc cgattcggcg gcgctgcctt
     4261 gcacgaggaa cacggcaccg acgcccacac cgacaacacc ggcaccgacg agcgcgccac
     4321 cgaccggatc tttccaccac gctcggcctt ctggtgtcga cggtcgcgtc tcggacggcg
     4381 gcgactggaa gctcgacgtc gtggccgtgt cggcgggtgc gtccgcggtc gtttcgtgac
     4441 tcgcatccgg cgcaacgtgc ggctccgtcg tgtcttgcgc ggtgtctggc ttgtccggct
     4501 tgtccggctt gtcgggctta tccggcttat ccggcttatc cggcttgtcc ggcttgtcgg
     4561 gcttcgccgc cttctccttg tcgagaatgt ccttgcactc gccgacctgg accttgatcg
     4621 cgtccttgtt ctccgccggc atgtccatcg cgaggagctt cgagtagagc tcgagcgcct
     4681 tgtcgcaatg ctcttgcttg cgctcgctct gtgcccacgc gaacaac
//
LOCUS       DATLBZ010000405        11131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9420479, whole genome shotgun sequence.
ACCESSION   DATLBZ010000405 DATLBZ010000000
VERSION     DATLBZ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11131
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9420479"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VMZ53_20380"
     CDS             complement(<1..415)
                     /locus_tag="VMZ53_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30885.1"
                     /translation="MFVRCSAALLALTSVASAEPFPDAVPGTGDDPILALTLLPEYVD
                     APLLAGISVRGGDPADTKVLFDGYEVPVVYFDHRALRSVFVGDAVERVYTEGTGVGYG
                     RGTSFLALVPRQRSTRGTYQLSNFDLSYAGPYPLAP"
     gene            complement(399..2696)
                     /locus_tag="VMZ53_20385"
     CDS             complement(399..2696)
                     /locus_tag="VMZ53_20385"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30886.1"
                     /translation="MPAGPSSLTQGDRQPAARVARLFASPGAVLVILPALVIAAGIVV
                     LLLGRRATRDTAETMARHQLVAQADAVKDDVAFALDQADPVLAMMGSIADTAMPTPDA
                     ITRLRDIVFKRPGIANASIAFPVGVLWGTFRDRKTGELQVFESRVGDTETNRTNYAIR
                     DGKLTLIATIVEKYDPRTRPHYKVAEEQKKRVWMPPRVLSNSGKTALTVSEPIYGTDG
                     KLAAVMTIDFEVAELSEFISKSPLAGARNVMFTADGTILAYPSAKIPDEDATRERRLL
                     GYHDFHDPALEALFKEMGSASPTQQRFMHLDADDGKYLAAITSISTGNAADGSARPTD
                     WYLATLVPEKTLFGAMERLGRDSIIASGSALLIAMGVALMFAWNILRMRRAVGAAREQ
                     ARSAEERAKQLGSYKLVGRLGAGGMGEVWRAEHQLLARKAAIKLVRPDILRRNEEHAA
                     LIQERFRREAQTLASMRSRHTIELYDYGVTDDGTFFYVMELLDGLDLSQLVRGFGPQP
                     AARVISIIGQACQSLGEAHHAGLLHRDIKPANLVLCRAADEVDIVKVLDFGIVHNITD
                     PIAPPVTAPELKPGRDNSGERLTTEGSVIGTPGYIPPEQATAAPLDARGDLYALGCVA
                     WYLLIGDEVYPGLDTEDQVLRAHVFEPIPSLRHKMRGWLPVELEQLITKCLAKRPEER
                     PEDARALAKALAAIEIPTEHQWTDTHAQMWWSSMQLPKASAPDSETVPNDGKLLVPNR
                     SATTQAPGSGTDARTIEARPSVRTL"
     gene            complement(2710..3426)
                     /locus_tag="VMZ53_20390"
     CDS             complement(2710..3426)
                     /locus_tag="VMZ53_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30887.1"
                     /translation="MKYALLLLLAATGCIDPIDARWQLDHDHVIAARATPPRIRAGDA
                     TNLDALVAHANAPVSIENPMTAEVATRGFEPYLEQRHDGAWSLESPSAEVLTAARPAL
                     SLADDEPVPVDVMLTFPHPMGLDRAALDPYRVKKTVWLGEPRANPELPAITIDGQPAP
                     AETDTIALPLDRDVYIAVDAPDDTRVNWLTNVGTLFQDDVAKAYVHVTAKDRLDGQLV
                     VVMRDAEGGVAWRVWSVLAR"
     gene            complement(3423..5561)
                     /locus_tag="VMZ53_20395"
     CDS             complement(3423..5561)
                     /locus_tag="VMZ53_20395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS30888.1"
                     /translation="MASADPSATSGRVVDTAGKPVRGAIVSIEGAPNGVTTDANGRFT
                     VSAEAGASLLVTADGFGMGLGAAGIDGDIILEPEAASETIEVKGERAPSAQGSARLGR
                     KELERIPGASNDVMRAMSAMPGVASYPLPLGQSGVVIRGSSPQDSKVLVDDFEVPMLY
                     HYLGFRSIILSEAIDSLEYVPGGFDVEYGRASSGIISVTTRAGDDKRGQQFETSAGEL
                     GVLGQGAFDRGRGHYMIAFRRSAIDLLLPMMIPDNVDLALTTVPRFYDEQMRFDYALG
                     KKWTLRVSSLGSDDSMELYASRDKNADKRFLNRGRFVRLTASAGYHDGPLTAKLSVSG
                     IAEQTMFERGEYQRLHVTSPALTTRAEVARSAAEYAGLKDVAWKTGIEVVSTRHAIDA
                     AVPAEHREGEPPSPDDPMDVSTQYKGNVDTSNVAGWTNVAASLDEHVRFSAGLRVDAY
                     TRVEDVAVQPRGELQFKLKKGLTLRTAAGAYSRPAEYQTELLDPELKAERSHQVMTGV
                     LYEPIEGVRAQGSLYYTQRTSLITRDLMTGELANDGRGTSYGAEILGTYNRGPWFGWL
                     SYSFSHSMRVDVPGGMARLFDYDQPHSLNIAASYKRGRWQFGGRWRLTSGLPTTPVVD
                     ATYDSDANIYYPVYGDVNSERAPIHHQLDLRIDRTSHWGPVKITQFLDVQNVYLNQTV
                     VGYFYGFDYTQRAAFHSLPILPTAGLRGEF"
     gene            5724..6710
                     /locus_tag="VMZ53_20400"
     CDS             5724..6710
                     /locus_tag="VMZ53_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30889.1"
                     /translation="MKSLAVIALLLATTPVLAKPARPGDVGGRRRPPDDQARPHPRPP
                     PPDLDEERVPPPPPPPDPVASGPMIDQRAELTDGGDPVRLVMTSGGKKPGIKLESKNG
                     SLELVTAEGVGTMKTAHGTTLIALEVDDAKEPFRIALFDGKKLTKVQRFARPAKQSNF
                     PFAIAATRTPEGFTVFFQEVETANPNEAHTFMLAVQKDGTAGELKEVQIPWALADAIW
                     NGNGYHLALYYTGEMRGARLSMVSTSADGQPQGHPDWATQPGAISDLHLVETDGKIRA
                     IYRNVDHMYSTDVTKIGQWGQVTAKTSDLGALPTTSLIAITAKGQATKVKTK"
     gene            complement(6713..7429)
                     /locus_tag="VMZ53_20405"
     CDS             complement(6713..7429)
                     /locus_tag="VMZ53_20405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30890.1"
                     /translation="MRRAALLVLVVAGCAADIDEPWELDHDRIIAVRAEPPGILAGQQ
                     SKLDMLVGFKASPVAERAPDFAQVVSPMSLADLVHNDGMGGWVVDAPTDERLAAVRAE
                     LKLSATDPVPLRIGIAAAWPTPVVSPMGNGFGAVKTVLLGLSVSNPALDGMMINGADP
                     GTTDLVVPAMGKTPLFVQADDEVNIVNWLTSAGTMHDFDLHSAYLTFEPEDMTEGQLC
                     VVLRDPFGGVAWRFWSLRAE"
     gene            complement(7426..9672)
                     /locus_tag="VMZ53_20410"
     CDS             complement(7426..9672)
                     /locus_tag="VMZ53_20410"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS30891.1"
                     /translation="MRFVTGLVMLGLGGVASAQPQDTPQVPEQPAPPPEAPAQTITVR
                     GRVINSLGRPVRGAKVSIEGSSPQATTDKQGWFRIEAPTGVNIVVEADGYEVGLAGVT
                     KPTLDDIVLLTNAQTAETIEVSGEAPPPTPGAAKLDRQELQRVPGTGGDVVRALTVMP
                     GVVNLQVPLGYSGVVIRGSSPQDSKVLVDDFEIPVLFHNIGFRAVVPAEAIEALDFIP
                     GGFDVGFGRASSGIVSLTTRPGSDQRSAQAEVSFIDGGLLAQGRAGKKTRYMMGLRRS
                     TIDFVLPNIIPESVDLSLTTVPSYWDGQFRVDHQLSDKWRLALSGVGTIDTFELYATK
                     DTDADTKRFYNQTRFLRLTGSGTYHDGPWTTKLALSTLLPKFVFEAGAYQFIDVRQPT
                     VTPRVDVVRTWAAAAGLTNVEWRFGGEAQVGRTTLKAALPPEQREGEPFMGYDPRDTS
                     IAFTGNFWVPDFAQWTSLTASLDPNIRATVGMRADEFARPGEVALQPRGELKVKVAPA
                     WTVRLSSGAYVRPPEFQSENVETTIDSEHSTQAIAGLQWEPTAGARVQASTYYTDRTN
                     LIVHEMDGTLSNAGVGTTVGGELLATYRGGPWFTWLSYSYSHSTRIDHPGDKRRLFTY
                     DQPHSMNAAASWKHKKWQLGGRFQLYSGLPYTPATGAILDSDRNVYIPLYGEVNGQRA
                     PLHHQLDLRVDYSWKWGPTALTFFVDVQNVYMNESIVTYFYGYDFTQRAAFTSIPIIP
                     SLGLRGVL"
     gene            9761..10369
                     /locus_tag="VMZ53_20415"
     CDS             9761..10369
                     /locus_tag="VMZ53_20415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30892.1"
                     /translation="MTENPTALVREVAGVSLATQGQYIIKRKFWSFFERVFRVWTMDG
                     QLIMYIKHPLLKLREEFTVYADEAQTRPLLFMKSRQVIAINFTYDVVDATTGMLLGSV
                     QKKGLASIIRDKFIISDANGMEVGYAEEQGASILRRFIPLLTSKHAIFVDGQQAAFIK
                     QIFRFFNKEFSVTTTPSRLDPRFVLAVALLALIAEARREDSN"
     gene            10379..>11131
                     /locus_tag="VMZ53_20420"
     CDS             10379..>11131
                     /locus_tag="VMZ53_20420"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HUS30893.1"
                     /translation="MRCLAAVWLIASACGRLSFDASSDGSITGDRPIDARPCTPVGHD
                     EDGDGIDDACDVCPHIADAAQLDSDGDSVGDVCDPEPTVGRQQLVMFDPFTTLDPQRW
                     TFVNDEHVANDELVFDAIGGSREIYRPYTLAQDWFIIGATTGTAGSGVQLIGLFTSAI
                     AGGIGYYCEMYDDGVDTSTMFTFTTDGTNFQHDGVNTWTSRLANGGGTFEYNIVGPNA
                     RCRGAWRGEVRLVEGVRPATITPQRFTVYVENV"
BASE COUNT         1915 a         3589 c         3709 g         1918 t
ORIGIN      
        1 gtggcgcaag tggatacggg cccgcgtagc tcagatcgaa attcgagagc tggtacgtgc
       61 cacgtgttga tcgctgacgc ggaacaagcg ccagaaacga cgtaccgcgc ccatatccga
      121 cccccgtgcc ttcggtgtaa acgcgctcga ccgcatcgcc gacgaagacc gaacgaagtg
      181 ctcgatgatc aaaatagacg acaggcactt catacccgtc gaacaggacc ttcgtatctg
      241 ctggatcgcc accgcgcacc gagatcccgg ccagcagcgg tgcgtcgacg tactcgggca
      301 acagcgtgag cgcgaggatc gggtcatcgc ctgtgccggg aaccgcgtcc gggaagggct
      361 cggccgatgc gacactcgtc agcgcgagca gcgcagcact acagcgtacg aacactcggc
      421 cgcgcctcga tcgtccgcgc atccgttcca ctgccgggcg cctgcgtggt cgccgagcga
      481 ttcggcacga gcagcttgcc atcgttcggc acggtctccg aatcaggcgc cgatgccttc
      541 ggcagctgca tcgatgacca ccacatctgc gcgtgggtgt ccgtccactg gtgctccgtc
      601 gggatctcga tcgcggcgag cgccttggcc agcgcgcgcg cgtcttcggg ccgctcttcg
      661 gggcgctttg cgaggcactt cgtgatcagc tgctcgagct cgacgggcag ccagccgcgc
      721 atcttgtgac gcagcgacgg gattggctcg aacacgtgtg cgcgcagcac ttggtcctcg
      781 gtgtcgaggc cggggtagac ctcgtcgccg atgaggagat accaggcgac gcagccgagc
      841 gcgtagaggt cgccgcgtgc gtcgagcgga gcggcggtgg cttgctcggg tgggatgtag
      901 cccggcgtgc cgatgacgga gccttcggtc gtgaggcgct cgcccgagtt gtcgcggccg
      961 ggtttgagct cgggcgcggt gacgggtggc gcgatcggat cggtgatgtt gtggacgatg
     1021 ccgaagtcca ggaccttgac gatgtcgacc tcgtcggcgg cgcggcagag cacgaggttt
     1081 gcgggcttga tgtcgcggtg gaggaggccg gcgtggtgcg cttcgccgag cgattggcat
     1141 gcttggccga tgatcgagat cacgcgtgca gcgggttgag gcccgaagcc gcgcacgagc
     1201 tggctgaggt cgagaccgtc gagcagctcc atcacgtaga agaatgttcc gtcgtcggtg
     1261 acgccgtagt cgtagagctc gatcgtgtgc cgcgagcgca tcgacgcgag cgtttgggct
     1321 tcgcggcgga agcgctcttg aatgagagcg gcgtgctcct cgttgcggcg aaggatgtcc
     1381 ggacgcacga gcttgatcgc agcttttcgt gccaggagtt ggtgctcggc gcgccacact
     1441 tcgcccatgc caccggcgcc gaggcgtccg acgagcttgt agctgccgag ttgtttcgcg
     1501 cgctcttcgg cggagcgtgc ttgctcgcga gctgcaccga cggcgcgacg catccggagg
     1561 atgttccacg cgaacatcag tgcgacaccc atcgcgatga ggagggcgga acccgatgca
     1621 atgatcgagt cgcgaccgag gcgttccatc gcgccgaaca gcgtcttctc ggggacgagc
     1681 gttgcgaggt accagtcggt cggccgcgcg gagccgtctg cagcgttgcc cgtgctgatc
     1741 gacgtgatcg cggcgaggta cttaccgtcg tcggcgtcga ggtgcatgaa gcgctgctgc
     1801 gtcggcgagg cggagcccat ctctttgaag agagcttcga gcgcggggtc gtggaagtcg
     1861 tggtagccga ggaggcggcg ctcgcgcgtg gcgtcctcgt cggggatctt tgcggatggg
     1921 tacgcgagga tcgtgccgtc agccgtgaac atcacgttgc gcgcgccggc gagcggcgac
     1981 ttgctgatga actccgacag ctcggcgact tcgaagtcga tcgtcatcac ggccgcgagc
     2041 ttgccgtcgg tcccgtagat cggctcgctg acagtcagcg cggtcttgcc cgagttggaa
     2101 agcacgcgcg gcggcatcca cacgcgcttc ttctgctctt cggcgacctt gtagtgcggg
     2161 cgcgttcgcg gatcgtactt ctcgacgatc gtcgcgatca gcgtcagctt gccgtcgcgg
     2221 atcgcgtagt tcgtgcgatt cgtctcggtg tcgccgacgc gactctcgaa gacttgcagc
     2281 tcgcccgtct tgcgatctcg aaacgttccc cagagcacgc cgaccgggaa tgcaatgctc
     2341 gcgtttgcga tgccgggccg cttgaacacg atgtcgcgca ggcgcgtgat cgcgtcgggt
     2401 gtcggcatgg cggtatccgc gatcgagccc atcatcgcga gcaccgggtc ggcttgatcg
     2461 agcgcgaatg cgacgtcgtc tttgactgcg tcggcttggg cgacgagctg atggcgcgcc
     2521 atcgtctcgg cggtgtctcg cgtggcgcgg cgaccgagca gcagcacgac gatgcctgcc
     2581 gcgatcacga gtgccggcag gatcacgagg accgcgccgg gcgacgcgaa caaacgcgcg
     2641 acgcgggcag ccggctggcg gtcaccctga gtcagcgagc tcggtccggc tggcaccgag
     2701 ccgatagtat caccgcgcga gcacggacca gacgcgccag gccacgccac cttcggcatc
     2761 gcgcatcacg acgaccagct gaccatcgag acgatctttt gcggtcacgt gcacgtatgc
     2821 cttcgcgacg tcgtcctgga acagcgtgcc tacgttcgtc agccaattga cgcgcgtgtc
     2881 gtcgggagcg tcgacggcga tatagacgtc gcggtcgagc ggcagcgcga tcgtgtcggt
     2941 ctcggcgggt gccggctggc cgtcgatcgt gatcgcgggg agctcgggat tggcacgcgg
     3001 ctcgccgagc cacacggtct tcttcacgcg atacggatcg agcgcggcgc ggtcgaggcc
     3061 catcggatgc ggaaacgtga gcatcacgtc gacgggcacc ggctcgtcgt ctgcgaggct
     3121 cagcgcggga cgcgccgccg tcagcacttc ggcgctcggc gactcgagcg accacgcgcc
     3181 gtcgtgacgc tgctcgaggt agggctcgaa gccgcgcgtc gccacctcgg cggtcatcgg
     3241 attttcgatc gagaccggcg cgtttgcatg cgcgacgagc gcgtcgaggt tcgtcgcatc
     3301 acctgcgcga atgcgcggcg gcgtggcacg cgcagcgatg acgtgatcgt ggtcgagctg
     3361 ccatcgcgcg tcgatcggat cgatgcagcc cgtggccgcg agtaagagca gcagcgcgta
     3421 tttcaaaact cacctcggag gcctgcggtc gggaggatgg gcagcgagtg gaatgccgcg
     3481 cgctgcgtgt agtcgaagcc gtagaagtag ccgacgaccg tttgattcaa gtagacgttc
     3541 tgcacgtcga ggaactgcgt gatcttcacc gggccccagt ggctcgtgcg atcgatgcgc
     3601 aggtcgagct ggtgatggat cggcgcgcgc tcggagttca cgtcgccgta gaccggataa
     3661 tagatgttcg cgtcgctgtc gtacgtcgca tcgacgaccg gcgtcgtcgg caagcccgag
     3721 gtcagtcgcc aacggccgcc gaactgccag cgcccacgct tgtagctcgc agcgatgttc
     3781 aagctgtgcg gttggtcgta atcgaacagg cgcgccatgc cgcccggcac atcgacgcgc
     3841 atcgagtgcg agaacgagta gctgagccag ccaaaccacg gcccgcggtt gtacgtgccg
     3901 aggatctcgg cgccgtagct cgtgccacgc ccatcgttgg cgagctcgcc ggtcatgaga
     3961 tcgcgcgtga tgaggctcgt gcgctgcgtg tagtagaggg agccctgcgc gcgcacgccc
     4021 tcgatgggct cgtacaagac gccggtcatc acttgatgcg agcgctcggc tttgagctct
     4081 ggatcgagca gctccgtctg gtactccgcc ggtcgcgagt aggcgcccgc cgccgtgcgc
     4141 agcgtcagtc ccttcttcag cttgaactgc agctcgccgc gcggctgcac agcgacatct
     4201 tcgacgcgcg tatatgcatc gacgcgcagg cccgcggaga accgcacatg ctcgtcgagc
     4261 gaggccgcga cgttcgtcca gcccgccacg ttcgacgtat cgacgttgcc cttgtactgc
     4321 gtgctgacgt ccatcggatc gtcgggcgat ggcggctcgc cttcgcgatg ctctgcaggc
     4381 accgccgcgt cgatcgcatg gcgcgtcgag acaacctcga tgcccgtctt ccacgcgacg
     4441 tctttcaggc ccgcatactc cgctgccgag cgcgcgactt ccgcacgcgt cgtcagtgca
     4501 ggcgacgtga catgcagacg ctggtactcg ccgcgctcga acatcgtctg ctcggcgatg
     4561 ccggacacgc tgagcttcgc ggtgagcggt ccgtcgtgat agccggccga cgcggtcaac
     4621 cgaacgaacc gtcctcgatt caagaaccgc ttgtccgcgt tcttgtcgcg gctcgcgtag
     4681 agctccatcg agtcgtcgga gccgaggctc gagacgcgca gcgtccactt cttgccgagc
     4741 gcgtaatcga aacgcatctg ctcgtcgtag aaccgcggca ccgtcgtgag cgcgaggtcg
     4801 acgttgtcgg gaatcatcat cggcagcagc agatcgatcg ccgagcggcg gaacgcgatc
     4861 atgtaatggc cgcgcccgcg atcgaacgcg ccctgtccga gcacaccgag ctcgccggcg
     4921 ctggtctcga attgctggcc gcgcttgtca tcgccggcgc gcgtcgtcac cgagatgatg
     4981 ccggagctcg cgcgtccata ctcgacgtcg aacccgccgg gcacgtactc gagcgagtcg
     5041 atcgcttcgc tcagaatgat cgagcggaag ccgaggtagt gatagagcat cgggacttcg
     5101 aagtcgtcga cgaggacttt cgagtcctgc ggcgagctgc cgcgaatcac gacgccggat
     5161 tggccgagcg gcagcggata gctcgcgacg ccgggcatcg cggacatcgc gcgcatcacg
     5221 tcgttgctcg caccgggaat gcgctcgagc tctttgcgtc ccagtcgcgc ggagccttgc
     5281 gctgacggcg cgcgctcgcc cttcacttcg atcgtttcgc tcgcggcctc gggctcgaga
     5341 atgatgtcgc cgtcgatgcc cgccgctccg aggcccatgc cgaagccatc cgcggtgacg
     5401 agcagcgaag cgcccgcttc cgcgcttacc gtgaagcggc cattggcatc ggtcgtcacg
     5461 ccgttcggcg cgccctcgat cgagacgatc gctccgcgaa ccggcttgcc ggcggtatcg
     5521 acgacgcgcc cgctcgtcgc agacggatcg gcagaggcca ccccactgcc caacaaaatg
     5581 acggccaccc aacgtcgcaa gccccgaaag gtgaccctgg atcatcgggt aggcaatgtg
     5641 aaaacgtcta aggatttctc gcgcctacag ctttctgcat gtaggtcggt cacgaaattc
     5701 cccgacggac gcacgtggtg atcatgaaga gcctcgcggt aatcgctcta ctgctcgcta
     5761 ccacgcccgt gctcgcgaaa cccgcaaggc ccggcgatgt cggcggaagg cggcggcctc
     5821 cggacgacca agcgcgcccg catccacgcc caccgccacc tgatctcgat gaggagcgag
     5881 tgcccccgcc tccgcctcca cctgatccgg tcgcgagcgg cccgatgatc gatcagcgcg
     5941 ccgagctcac ggacggcggt gatccggtgc gcctcgtcat gacgtcgggc ggcaagaagc
     6001 ccggcatcaa gctcgagtcg aagaacggat cactcgagct cgtgacggcg gaaggcgtcg
     6061 gcacgatgaa gaccgcgcac ggcacgacgc tcatcgcgct cgaggtcgac gacgcgaaag
     6121 agccattccg catcgcgctc ttcgatggca agaagctgac gaaggtgcag cgcttcgcgc
     6181 gtcctgcgaa gcaatcaaac tttccattcg cgattgccgc aacgcgcacg ccggaaggct
     6241 tcaccgtctt ctttcaagaa gtcgagaccg cgaatccgaa cgaagcgcat acgttcatgc
     6301 tggcagtcca gaaagacggc acggcgggtg agctgaaaga agtgcagatt ccgtgggcgc
     6361 tcgccgacgc gatctggaac ggcaacggct accacctcgc gctctactac acgggagaga
     6421 tgcgcggtgc gcggctctcg atggtgtcga cgtcggcgga cggtcagcca cagggccatc
     6481 ctgattgggc gacgcagccc ggcgcgatca gcgaccttca cctcgtcgag accgacggca
     6541 agatccgcgc gatctaccgc aacgtcgacc acatgtactc gaccgacgtc acgaagatcg
     6601 gccagtgggg ccaggtcacc gcgaagacca gcgacctcgg cgcgctgccg acgacctcgc
     6661 tcatcgccat caccgccaag ggacaagcga cgaaggtgaa gacgaagtaa ggctattcgg
     6721 cgcgcagcga ccagaagcgc cacgcgacgc cgccgaacgg atcgcgaagc acgacgcaca
     6781 gctggccttc tgtcatgtcc tcgggctcga acgtcaggta ggcgctgtgc agatcgaagt
     6841 cgtgcatcgt gccggccgac gtcagccagt tgacgatgtt cacttcgtcg tccgcttgca
     6901 cgaacagcgg cgtcttgccc atcgccggta cgacgagatc cgtcgttcct ggatcggcgc
     6961 cgttgatcat catgccgtcg agcgccgggt tcgagacgga gagaccgagg agcaccgtct
     7021 tgactgcacc gaagccattg cccatcggcg agacgacggg cgttggccat gcggccgcaa
     7081 tgccgatgcg cagcggcacc ggatccgtcg cggacagttt cagctccgca cgtaccgcgg
     7141 cgagacgctc gtcggtcggc gcgtcgacga cccagccgcc catgccgtcg ttgtgcacga
     7201 gatcggcgag cgacatcggc gagacgactt gcgcgaaatc gggtgcgcgc tctgcgaccg
     7261 ggctggcttt gaagccaacg agcatgtcga gcttcgactg ctggcccgcc aggatgccgg
     7321 gcggctcggc gcgcacggcg atgatgcgat cgtggtcgag ctcccacggc tcgtcgatgt
     7381 cagccgcgca accggcgaca acgagcacga gcagggcggc gcgtctcaca gcacacctcg
     7441 cagaccgagc gacggaatga tcgggatcga cgtgaacgcc gcgcgttgcg tgaagtcgta
     7501 gccgtagaag tacgtcacga tgctctcgtt catgtagacg ttctgcacgt cgacgaagaa
     7561 cgtcagcgcg gtagggcccc acttccacga gtagtcgacg cgcagatcga gctgatggtg
     7621 gagcggcgcg cgctggccgt tcacttcgcc gtagagcggg atgtagacgt tgcgatcgct
     7681 gtcgaggatc gcgcccgtcg ctggcgtgta cggcagcccc gagtagagct ggaagcgccc
     7741 accgagctgc cacttcttgt gcttccagct cgccgctgcg ttcatgctgt gcggctggtc
     7801 gtacgtgaag aggcggcgct tgtcgcccgg atgatcgatg cgcgtcgagt gcgagtacga
     7861 atacgagagc cacgtgaacc acgggccgcc gcgatacgta gcgagcagct cgccaccgac
     7921 ggtcgtgccg acgcccgcgt tgctgagcgt gccgtccatc tcatggacga tcaggttcgt
     7981 gcgatcggtg tagtacgtcg acgcctggac gcgcgcgcct gcggtgggct cccattgcag
     8041 gcccgcgatc gcctgcgtcg agtgctcgga gtcgatcgtc gtctcgacgt tctcggattg
     8101 gaactccggc ggccgcacgt acgcgcccga cgagaggcgc acggtccatg caggcgcgac
     8161 cttcactttg agctcgccgc gcggctgcag cgcgacttca cccggacgcg cgaattcgtc
     8221 ggcgcgcatg ccgaccgttg ctcgaatgtt cggatcgagg ctcgccgtca gcgacgtcca
     8281 ctgcgcgaag tctgggaccc agaaattgcc ggtgaacgcg atcgacgtat cgcgagggtc
     8341 gtagcccatg aacggctcgc cttcgcgttg ctcgggaggc agcgcggctt tgagcgtcgt
     8401 gcggccgact tgcgcctcgc cgccgaatcg ccactcgacg ttcgtgagtc ccgcggccgc
     8461 cgcccacgtg cgaaccacgt cgacgcgcgg cgtgaccgtc ggctggcgca cgtcgatgaa
     8521 ttgataggcg cctgcttcga acacgaactt cggcaacagc gtcgagagtg cgagctttgt
     8581 cgtccacgga ccatcgtgat acgtgcccga gccggtgagg cggagaaacc gcgtctggtt
     8641 gtagaaccgc ttcgtgtcgg cgtcggtgtc cttcgtcgcg tagagctcga acgtgtcgat
     8701 cgtgccgacg cccgacagcg cgagcctcca cttgtccgac agctgatgat cgacgcggaa
     8761 ctgaccgtcc caatagctag gcaccgtcgt gagcgagagg tcgacgctct ccgggatgat
     8821 gttcggcagg acgaaatcga tcgtcgagcg acgcaggccc atcatgtagc gggtcttctt
     8881 gcccgcacgt ccttgcgcga gcagaccgcc gtcgatgaac gagacctcgg cctgtgcgct
     8941 gcgctggtcg ctgccgggac gcgtcgtgag cgagacgatg cccgacgatg cgcggccgaa
     9001 gccgacatcg aagccacctg gaatgaagtc gagcgcttcg atcgcttctg cgggcacgac
     9061 cgcgcgaaag ccgatgttgt ggaacagcac cggaatctcg aagtcgtcga cgagaacttt
     9121 tgaatcctgc ggcgagctgc cgcggatcac gacgcccgag tagccgagcg gcacttgcag
     9181 attcacgacg cctggcatca ccgtgagcgc gcggacgacg tcgccacccg tgccgggcac
     9241 gcgctgcagc tcttggcgat cgagctttgc cgcaccgggc gtcggtggtg gcgcttcgcc
     9301 cgagacttcg atcgtctcgg cggtctgcgc gttcgtgagc agcacgatat cgtcgagcgt
     9361 aggcttcgtg acgcccgcga gaccgacttc gtagccatcg gcctcgacca cgatgttcac
     9421 gccggtcggc gcctcgatcc ggaaccagcc ctgcttgtcg gtcgtggcct gagggctcga
     9481 gccctcgatg ctgaccttcg caccgcggac gggcctgccg agcgagttga tgactcgacc
     9541 tcgaacagtg atcgtttgcg caggggcctc cggaggtggc gccggctgtt ctgggacttg
     9601 cggcgtgtcc tgtggctgcg cggacgcaac tcctccgagc ccgagcatca ccaaaccggt
     9661 cacaaaccgc atgggtgcgc gaaaacagca atcaccatgc cgcctactga tcatggatca
     9721 gctgcatgtt agaccagcgc gtgtgtgaag gagaccgacg gtgactgaaa atccgacagc
     9781 gctagtccgg gaagtcgcgg gtgtcagtct ggcaacgcag ggccagtaca tcatcaagcg
     9841 gaagttttgg tccttcttcg agcgcgtgtt ccgcgtctgg acgatggatg ggcagctgat
     9901 catgtacatc aagcacccgc tcctgaagct ccgcgaggag ttcacggtct acgccgatga
     9961 agcgcagacg cggccgctgc tgttcatgaa gtcgcgacaa gtgatcgcga tcaacttcac
    10021 gtacgacgtc gtcgacgcga caaccggcat gctgctcggc tcggtccaga aaaaaggcct
    10081 cgcctcgatc atccgcgaca aattcatcat ctccgatgcg aacggcatgg aggtcggcta
    10141 cgcggaagag cagggcgcat cgatcttgcg ccgcttcatc ccactgctca cgtcgaagca
    10201 cgcgatcttc gtcgatggcc agcaggccgc gttcatcaag caaatcttcc ggttcttcaa
    10261 caaggaattc tcggtcacga ctacaccgtc gcgcctggac ccgcggtttg tgctcgcggt
    10321 cgctctcctc gcgttgatcg ccgaagcgcg ccgcgaagat tcgaactaag atcgtcccgt
    10381 gcgctgcctc gctgccgtgt ggctgatcgc gtcagcctgc ggtcgcttgt cgttcgatgc
    10441 gtcgtcggac ggcagcatca ccggcgatag accgatcgat gcgcggccat gcacgcccgt
    10501 agggcacgac gaagacggcg acggcatcga cgacgcctgt gacgtgtgcc cgcacatcgc
    10561 cgatgccgca cagctcgaca gcgacggcga ttcggtcggc gatgtctgcg atccggaacc
    10621 gacggtaggc cgccagcagc tcgtgatgtt cgatccgttc acgacgctcg acccgcagcg
    10681 ctggacgttc gtcaacgacg agcatgtcgc gaacgacgag ctcgtcttcg atgcgatcgg
    10741 cgggagccgt gagatctatc gcccgtacac gcttgcgcag gactggttca tcatcggcgc
    10801 gacgacaggc actgcgggtt ccggcgttca gctcatcggg ctcttcacat cggcgatcgc
    10861 cggcggcatc ggctactact gcgagatgta cgacgacggc gtcgatacgt cgacgatgtt
    10921 cacgttcacg accgacggca caaacttcca gcacgacggc gtcaacacgt ggacgtcgcg
    10981 gctcgcaaac ggcggcggca ccttcgagta caacatcgtc ggcccgaacg cgcgttgccg
    11041 cggagcgtgg cgcggcgagg tgcggctcgt cgagggcgtc cgaccggcga cgatcacgcc
    11101 gcagcggttc accgtctacg tcgagaacgt c
//
LOCUS       DATLBZ010000406        28365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2721671, whole genome shotgun sequence.
ACCESSION   DATLBZ010000406 DATLBZ010000000
VERSION     DATLBZ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 28365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28365
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721671"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VMZ53_20425"
     CDS             <1..361
                     /locus_tag="VMZ53_20425"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF026049.4"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HUS30894.1"
                     /translation="LTVARGMIASLVFDVARMRAAIDQGYLVATEVADYLVTKGVAFR
                     EAHDISGHLVRTASDRGVELGALDLATLKAAHASFEPDLADWLDPARAIDRRDVVGGP
                     ARARVLAEIDRIASELE"
     gene            374..1630
                     /gene="lysA"
                     /locus_tag="VMZ53_20430"
     CDS             374..1630
                     /gene="lysA"
                     /locus_tag="VMZ53_20430"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HUS30895.1"
                     /translation="MGPFQYRDSQLHCEDVSLTKIAEEVGTPVYVYSRGDIERAYKAF
                     DAALEGIPHEVCYAVKACSTLGVLGVLVELGAGADIVSIGELYRWLKAGGDADSVVFS
                     GVGKSEGEMKGALDANIGCFNVESGEELVVLDRVAQANNKRAKISLRVNPDVDPQTHP
                     YISTGLKQNKFGVSMQEARELFKSAAKMKGIAVAGVDFHIGSQLTKTSPFTDAIARLV
                     ELVQLLAKDGIKLEHVDIGGGLGIDYGKGEHVPSPADYGKAVRSALAPLTDLGVKLLT
                     EPGRVIVGQAGALVSRVLYRKKNEAKHFTIVDAAMNDLMRPALYGSHHPIKPVKNPDR
                     PAIVSDVVGPICETGDFFARDRELPELEQGELVWIGAAGAYGSAMASNYNTRPRAPEV
                     LVSGSSYTVIRTRETYEQLVANEKLG"
     gene            complement(1627..4836)
                     /locus_tag="VMZ53_20435"
     CDS             complement(1627..4836)
                     /locus_tag="VMZ53_20435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30896.1"
                     /translation="MGDPEEPTVKERRSSADTAAGHGNSSPKNTDLEAPRASAFDTND
                     RFETRGELGRGGMGRVDDAYDRALGRQVAIKHVLSHAEVDLARFEREARITARLEHPG
                     IVPVHDVGRSEDGSPYYIMRRVDGEPLSELIADKPFAERVRLVPNILAACDAAAFAHS
                     HGIVHRDIKPTNILVGPFGETLLIDWGLAREVSTSDEPASAQPISDGNLTRAGTVAGT
                     PGFMSPEQARGETVDARADVFALGATLFYVLAGQLPWHGGSTEMLQHVGAGRPPDWRL
                     MPAEVPADLRAIVEKAMSRDLADRYADATALAADLRRFTLGNLVAAYNYGWFARVVRF
                     VRKHRAAASIAAISIVALAVVGIISFRRVVAERDAANQARTLAEARQREARAAADRLL
                     VQHAHELVATDPLEAIAALRTLAPDSLRWRDAWVVAMEAKLAGIPIGFRLPHEPGLMQ
                     VSADRRHIFWMSLFSPTLLLIDLPARSTRELALGTRIFKPTWFDATHLIATTDAETIL
                     LDITSGQRRAWPFVAKEIASNRAGLARIFAGTTLYELTASDATPRVVAEGLTGYESAA
                     DLSASIYIRGGKRNFVVGDRSYPLPDLTADEHKYATIYAISPTTVVMAHGYMSAAWDL
                     VDGKLLKRGEWPQQVLVTGVFSAGNYLYMTSGEGVSILGRAATREPVQLQSQWRTQLG
                     FLAVYSDGSIVLRDGMGTMALGKRHASYVRVDASPDGRTIAALTTAGEVLVWDLDEIR
                     PELIALERTESPRQLSNADLWTTTMGEGIYRIDLRTHERVRALDIETPLVESAYDRIH
                     DWLLVRSHHHGLTILARDRAPVSIEKVDLFSFGQAGIVYTRKGYVTLWTPNGETEFGS
                     FSPVPTIAAPGKSCVYVLAGDSELLRIDIATRRTERVTFPVTYIAIDDAETAWFAAGG
                     KAYRWDRAAPAPVALPSPEIVDSVGITQHGALFHSSRALVLYGKQPWPRVFSIPTVAA
                     LAIDKDLLATLSSQGVVVVLDLETGGSFTLPGHHDMTHALVASVTHVGMVNFRHQPVA
                     SVVALFSMSVPREPAELQKWLANVTNARPIEGSEAVAFP"
     gene            4870..6165
                     /locus_tag="VMZ53_20440"
     CDS             4870..6165
                     /locus_tag="VMZ53_20440"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HUS30897.1"
                     /translation="MTWVPWLCLALILGACGDNAEGEDRQGGDTTVDNRSWDAFRQPA
                     ANLSAGERNRFQAGRSPFDFRWDAPQLGTLFNNVSCLGCHAVNGRGASQIGMGPASQA
                     LVRVSLSTGDPGDPGGPIPLPGYGTQLQDHATVGLPEVRVMLTWIETSMMYDDGELIA
                     VREPRLDIVGANSTPLPQGTRFSYRTAPPVIGLGLLEAIPQSTLEALADPDDADLDGI
                     SGRTNQVWDPEKGATVMGRFGWKANTSTLHLQCAAAAANDIGLSNAVFPELDQQRDLN
                     DQQLEDMVFFMATVGVPAAAPRDAKALHGRALFRDFGCASCHVPTLETGDHPIAAVAN
                     QRIHPYTDLLLHDVGDRLTDARPDFVAEGVEWRTPPLWGIGLTKTVFPMATYLHDGRA
                     RSLDEAILWHGGEAMSAREAFRTANREDRIALIAFLETL"
     gene            6182..7483
                     /locus_tag="VMZ53_20445"
     CDS             6182..7483
                     /locus_tag="VMZ53_20445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30898.1"
                     /translation="MEPDEKGATISDGGPSFDSDRDGALIGADVGGYVVEGELGRGGM
                     GVVYQATHPMIGKRVAIKVLKPSLSNNPATVERFVQEARSVNQIGHPNIVDIFAFGTL
                     PDGRSYLIMDLLVGESLRKRVKRGALPVEDTVRILDEVASALAAAHDKGFVHRDLKPD
                     NVYLVDHHGHVDVKLLDFGLAKLLVPSGLSGRAFRTATGAMLGTPDYMSPEQLRATGV
                     DHRTDIYALGVVAFEMLAGERPRRYSDGSFELRGSTVPRYLSEVHGAPLALAELVDAM
                     LALDPEQRPSIVAIRTVLKRVKNGQAEAVRAIGTAPSLGISPSLVGARPVVATPLSGT
                     PTISEVPSRASAPSLPPVSQPPPQYTSQRPNIGRTRPRPSGLAKSVHPATKLGVAPPP
                     ERASKPHPVAAARRGEGKLWLFLGIGLIVISVIALVAVLAS"
     gene            7504..9255
                     /locus_tag="VMZ53_20450"
     CDS             7504..9255
                     /locus_tag="VMZ53_20450"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30899.1"
                     /translation="MRDSDDDRPTDSHDVVGEDDDEFPPDGETVTTVDDHLSPGTNVG
                     GYVIENELGFGGMGVVYAATHPVIGKRAAIKVLKPELSKTPAAVERFVMEARAVNQIG
                     HPNIVDIFAFGALPDGRSYYVMDLLAGESLRRRLKRGPLHVSEAVSILDEVASALMAA
                     HDKGIIHRDLKPDNIFMVTIPGRWPEAKLLDWGLLKLTSAQSSVSSGKYRTLAGSVMG
                     TPVYMSPEQARASDQVDLRTDIYALGVMAYELLAGVVPFKKGSSIDTLLAHQDEPVPS
                     LQEKCPTLPVELVQLIEAMLAKEPDDRPTLAAVRAVIKRLKGTKIPTMTAAGLEITPL
                     PGKPFVEPKTMEFVDPPTRRQQPHVIAAPKTAEHEAVFAEPPTMEQPRSFDPKAAFVE
                     PPTLEQPRAPSTRANTRPTRPHPSNPFGETPTTVAPASFDPKAAFAMDAVAGLDEVRR
                     SSAVTSPSMPAPVTGDVTSPSLPLPAPPSTQHRMDSQMSSGDSLPYQSMNVSSPQTTL
                     AGHSMPMPNASRPPMYTTPLPGSIPPTYQQQPPMRPSHSSIPPANQQASGGNRLLVII
                     IAAVIASAIGITIALLT"
     gene            complement(9252..10166)
                     /locus_tag="VMZ53_20455"
     CDS             complement(9252..10166)
                     /locus_tag="VMZ53_20455"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUS30900.1"
                     /translation="MKKLLKRIAIGFALLLVFGTTAFAVAVVARENRTFEAPYPDIHA
                     SGDPTVIARGEYLVRGPAHCVACHGEDLRGGLKFSLPVGTIYARNITPDEATGIGRYS
                     DREIARILRFGVHPTGRAMLPFMPFANLADDDLTAVVSYLRSRSAIHNRVPAHDFNVI
                     GRVAKAFILEPKGPTVTPPKHVEPAVTAEYGEYIANTVANCGGCHTKRDMRTGGAIGV
                     AFAGGQTVESHRTPGVTFVTPNLTPDPATGHITAWSEEMFVARFKNAVDTASPMPWGE
                     FRTLSDGDLRALYRYLRSQPPAKLGQQL"
     gene            10226..10573
                     /locus_tag="VMZ53_20460"
     CDS             10226..10573
                     /locus_tag="VMZ53_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30901.1"
                     /translation="MAVIAVSDEPSIAVLVAAAARGDRRAEAVLCRRFAPAVRTFWRR
                     RLHGDAIDEASQDVLLRFVQALRTGAVTEPERVGGFVLGICRNLARERARQQERRAEL
                     WAEFGDALASIDE"
     gene            10598..10801
                     /locus_tag="VMZ53_20465"
                     /pseudo
     CDS             10598..10801
                     /locus_tag="VMZ53_20465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            10798..11346
                     /locus_tag="VMZ53_20470"
     CDS             10798..11346
                     /locus_tag="VMZ53_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30902.1"
                     /translation="MSRLQDLDAYLTREMSDADADALEEAMFDAPDDADLAFFDRLMR
                     HGRRLVEHGTFEMGVLQSKIDELITKGHTVQVLDAGPAGQPQQTLVLSRDAEFIVTKL
                     HLGRTDIARVDVEITIVEHDVTKTIKDVLVDPSDGIIYGLCERPLAHLALGAGAAHTK
                     VRRVDGARDVLGEWHTVGQLAV"
     gene            complement(11348..13354)
                     /locus_tag="VMZ53_20475"
     CDS             complement(11348..13354)
                     /locus_tag="VMZ53_20475"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUS30903.1"
                     /translation="MEQRRSACPLDCPDLCGLTVTVDNGRVVDVEGDRRGPLTDGFIC
                     GKVRKIADHLYGDDRVLHPMIRTGAKGSGQWRQATWDEALDHVAARLAQIRATSGSTA
                     LLPYHYGGSNGWLTEGSLATRFFRRFGSSNLDRTFCAAATVAAARGLYGAMPGVALED
                     FVHSKLIVIWGNNPAATGIHLVPIIDKAREAGAKLVVVDPRRTPLARRADLHLALRPG
                     TDLPVALSIINALFERGHADKAFLARHALQVDELKKRASEWSLELAAQEAQVPVDDLD
                     RFVELYAATSPAVIRAGWGMERNRNGGSAIAAVLAIPAVAGKFTRGGGFTMQNSDVAW
                     TVSSETAIGEPPTTARMINMGQIADALATLRDPPIECLFVYNCNPAATAPAQHQLLEQ
                     LKREDLFVVVHDQVWTDTAKVADVVLPATAFLEHRELRRGYGTMRMFDSPAVAVPPGE
                     ARSNNQLFGELLRRFDLVRPGDAMTDDELVIATFAASEQGATLRTQLLRDEVASPPGG
                     PRPQPFADIFPATPDKKIHLVPDALDQQAKGLYRYKRDPRTDEFPLALISPALATQIS
                     STFGQLRSAPGALEMSPADAAARGIANGANIRVWNALGEVRCIAKISTDVRDGVCVLP
                     KGLWRKHTRNGYTSNALIPQTTADLGGQAAFNDARVQVALDAGE"
     gene            complement(13373..15115)
                     /locus_tag="VMZ53_20480"
     CDS             complement(13373..15115)
                     /locus_tag="VMZ53_20480"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS30904.1"
                     /translation="MRVWLASAVAVCAALGSARAQQRPDDSKPVPDDFTPGKRIDVRG
                     EDRGFIGGTQVLEIDRCPEHDEMTPDQLRGAAAEHFDRGEVLYVQGDYHGAVTELVSA
                     YCFAPYYAVLKDIGQAYERELEYEKAIAYFEKYVLAVPPDAKRTGQCGNDPQIEKENV
                     SARIKVLRNLRAKVRVNTDPPGSKITLLDYQGTVAARGSSGDELAVVGGHYTVVVNQE
                     GFEEVSTDVDVEIGKPYTIFSKLEALKGRLRVRVVPADSRLFLQYPNGDKLQVGNVGT
                     GAFESALPGGKYRVFAEANGRVTVARDIEVLADKDTPISMDLPPVPQFGRRQLIVYST
                     VAGSVAGATLAGATENAGLVLTGFGAGLAGGFFIGYYATPSDIPLGTSSLTITSSLIG
                     AVVAVDTASLFTEQTNVRVPMVGAGLIVGAGIGAYAGSRTHITPGDAAVINSGAVWGT
                     ATGTLFAGSFEADQKVSAGLVLSGLGMGTLGGVLVTRYFHVSRGRAALIDVGGVIGAL
                     VGFGTQNILSDQTNAAGTSTASETNYTLGGMITGLLVAGVLTRNMDAPKLAVTPTVSK
                     TQGGTTTFGVGGDW"
     gene            complement(15112..16839)
                     /locus_tag="VMZ53_20485"
     CDS             complement(15112..16839)
                     /locus_tag="VMZ53_20485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS30905.1"
                     /translation="MSTGSGDKPGGGSSPGRPAADTPGRLVVRSLDGASDTSMGASDT
                     AVAVATKSSHGMNDSSEKSLSATEAGPTPAAGMQALQPSATELTGVTLSGRYLVTRKV
                     GQGGMGAVYEATHTLIGKRVAVKVLLEKYAQREAIVKRLKQEAQLASSIGNEHIIDIT
                     DFGNTDDGRTFVVMEFLEGESMAECLAKEPKLPEARILRIATQTASALAAAHAKGVVH
                     RDIKPENIFLLKRKEMDFVKVVDFGISKSLRASGEEEEAVRLTQTGMVLGTPLYMSPE
                     QARGDEDLDHRVDIYALGVIMYESATGRVPFGGNNYLSVISQVLNETPKPVREIRPEL
                     SDEFEAIVSKALAKDRNDRYANATDMLADLNALLDDPTHSTERAKITGPRRLLPKQVQ
                     KIPRIAWAISLAGIAVGAVTIAVVLLMGSKQKKQVPQPQVALHSDAGVQALPVIDAGE
                     AKPAFETIKLTITSDPPKADIYRESELVGKAPVEIELVKNNDIVNIYAELPNGFVGQT
                     KINPAERKNGATVPIKLKKQSGSGAKIIRPTGKGSASTGTTQTGTTNHTAGELSNNPY
                     AGSGTVQNK"
     gene            16980..18074
                     /locus_tag="VMZ53_20490"
     CDS             16980..18074
                     /locus_tag="VMZ53_20490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUS30906.1"
                     /translation="MIVLAARADLFRLLGDEDRLRLLALCAEEELTVGELATLLGESQ
                     PQVTKKTQPLREFGLLSARKDGTRTLLKTDVVEARSDKGRTQLPQPAGLTTKGRTQLP
                     QPAVRNSDTDGLADALDPVIAAALEEGRALCAKDGSLARIPRVIAAREEVSRRLFDQP
                     QDERPAPAVDPTLLSWLPLLAPLLPGRALAVDIGTGEGALLPLLSPLFDRVIAVDRSA
                     ARLARAASNIASWDLPNVRLREGAVDDAALAEEVYRFTRTDDPRGPRGADLVVMARVL
                     VHASRPQDVIAQAAQLVRPGGHLILIDYVPHDDESLREQGHVWLGFEPGKLRTWLEAA
                     RLQTVAVQPFSQPLLQLAVGRKPGDHHGHS"
     gene            18061..19518
                     /gene="ahcY"
                     /locus_tag="VMZ53_20495"
     CDS             18061..19518
                     /gene="ahcY"
                     /locus_tag="VMZ53_20495"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HUS30907.1"
                     /translation="MDTRNEFPAFKIKDLTLAELGRKKIRMAEKEMPGLMSLREEYKG
                     KSPLKGARVAGCLHMTIETAVLIETLVSLGAEVTWTSCNIYSTQDEAAAAIAKAGVPV
                     FAWKGENLEDYWQCIEMQLTAFQGGKGPNLILDDGGDLTLLVHKGVEAEKKGSAPDPK
                     TATNEEMRVIYSVLTESLKKSKDRFTTMAKEIKGISEETTTGVHRLYEMGKTGSLLFP
                     AINVNDSVTKSKFDNLYGCRESLFDGIKRATDVMVAGKVVVVAGYGDVGKGCAHAARG
                     LGARVLITEIDPICALQAAMEGYEVVTMEEAAPMADIFVTATGCCDVIRAEHFQVMKD
                     EAILSNIGHFDSEIQVAWLENNKEIKEENIKPQVDQFIFPNGKRITLLARGRLVNLGC
                     ATGHPSFVMSNSFTNQTIAQLALWTNAQKGKDEMTGMKFEAGQVYVLPKKLDEKVARL
                     HLKKVGVKLTTLTKEQSDYLGVAVDGPFKPDHYRY"
     gene            complement(19540..20019)
                     /locus_tag="VMZ53_20500"
     CDS             complement(19540..20019)
                     /locus_tag="VMZ53_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30908.1"
                     /translation="MDGNADKIGQIVGIVITGLIGFGVYWSLRGGKLKLAPGETRIAY
                     IYANWPGHRFGGLIVAGSPSDGKLVLTTQRLIFTNPTEGKVALAVTVNEIQSVAKGSK
                     GPLMTLELGYKTPKGKLKSASFTQVLSNPTIAIDPKRELPIGMFIDKITAWREGRAA"
     gene            complement(20029..21048)
                     /locus_tag="VMZ53_20505"
     CDS             complement(20029..21048)
                     /locus_tag="VMZ53_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30909.1"
                     /translation="MRRVAAVALLLIACGDDDSGSGEYVSVADFDTAYKDAECTYLVR
                     CGLFPDQATCTSAALFAGGAYSLSPDVRAEIAAGRVLYNGNAVHACFDAIANATCDKT
                     DEIGRAPISACYTFTRGTLAADAPCLTDAECISQDCRALVADGLCEMGACNGDTPPSL
                     ELPINGEPCNRIVGCGRGSYCDSSTDVCTPLKIAGADCVDTRECGFGLGCAGAPRTCK
                     TLPGPNEACPDGVCRDEGLHCGGAVCKALGLVGATCSSSTDCSPYFPCDFATTMCKRP
                     PSLGEACASSNSRCFDEKSYCDGATLKCVAAKPDGSACSTSLQCESGNCDFNTSTCVT
                     QSCSG"
     gene            complement(21061..21480)
                     /gene="arfB"
                     /locus_tag="VMZ53_20510"
     CDS             complement(21061..21480)
                     /gene="arfB"
                     /locus_tag="VMZ53_20510"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HUS30910.1"
                     /translation="MHDIVVTPRLTIPAGELGIAFARSGGPGGQNVNKVASKVELRWN
                     PTTSTAVADADRSYLVSRLANKLTTEGDLIVTSTLTRDQIKNRNDAMSKLALIVRAAL
                     DRPKKRFATKPTRSSKRRRVEGKRHRAEIKKNRRADD"
     gene            complement(21505..22314)
                     /locus_tag="VMZ53_20515"
     CDS             complement(21505..22314)
                     /locus_tag="VMZ53_20515"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HUS30911.1"
                     /translation="MLRYKETEDIDARFSEVERTVTAWRQALTTANDVARTEFNNRLR
                     ISLIHHDAALEGEVLSYSEIKAAVDPSIISDPSLIPSYEEIRRFDDACNFAAENAGNK
                     KKPFKLDTVREIYSILSPDEKAKGLPFRKENPLHRLYYHDIAPPEKILPAMRKLGEWI
                     DDPTSKHTHVIERVASLHSKFMAIFPWAKESGRTIRIASNQLLQEAGYPIAIIHSIDR
                     QRYYEALRGDHMALTSLYLEAVQTTAETELRVYEEAQKAPPKKRSKKSGAA"
     gene            22471..23535
                     /gene="rsmH"
                     /locus_tag="VMZ53_20520"
     CDS             22471..23535
                     /gene="rsmH"
                     /locus_tag="VMZ53_20520"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HUS30912.1"
                     /translation="MSAAGSSGSDFAHESVLLAETIANLSPRNDEIYVDCTLGGAGHS
                     SAILAAAPNARLIGIDRDPAALAAARTRLESGLTTKGRTQLPQPGGQTTLVHGEMGDI
                     RSILADLGITQVHGFLADLGVSSPQLDHADRGFSFSKEGPLDMRMDPTRGPTARDLLR
                     ATSEVDLASLISDLGEERHARRIARTIKEALRENEDALRTTTELAALVTKCIPIVEQR
                     KSKIHPATRTFQGLRIAVNAELQQLERFLDAFPDLLAPDGRCVVISFHSLEDRLVKNA
                     FRDLAWSTSLPPKLAQQAGERLEPVVEVLTRKAVFASDEETARNPRARSARLRACRRT
                     AAPNVPMGGSAGDPHLRGRD"
     gene            complement(23679..24143)
                     /locus_tag="VMZ53_20525"
     CDS             complement(23679..24143)
                     /locus_tag="VMZ53_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30913.1"
                     /translation="MRRLSLLLLATLSLVSTSGCTTPQARRAHRAGEVAAAGGLVGML
                     AAGVTASAIPAHDTQILTVGLAFIPISIIGALVFIATDDKANEHAAPYLTRRERHRAE
                     AWELTKQAAAAARSADCTQVQAIDPRVRDLDLEFHGIVFLRDVAIRRCLRPQ"
     gene            24232..24591
                     /locus_tag="VMZ53_20530"
     CDS             24232..24591
                     /locus_tag="VMZ53_20530"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HUS30914.1"
                     /translation="MTSPGAASVYRIFKRSDGAASVRSVVIAMALVACVLTGLGVVRV
                     TRQHEVLALGYQLDREAEHLRQQKEINRRLQVELATQSSPDRVRRLAAQLGMTPVPPD
                     RIRIIDVPSRKKVAAAP"
     gene            24588..26675
                     /locus_tag="VMZ53_20535"
     CDS             24588..26675
                     /locus_tag="VMZ53_20535"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HUS30915.1"
                     /translation="MTRDKKLKPMTPGVSSSARVRAYLAGAVVSLGLFGVAMKAWALQ
                     VDDGAKYREAAARQHAMRLDIPAPRGEVSDRMGRPLAITADADSVWANPREVHDVAGT
                     AEKLAALLHGDAGVLESRLAGDRGFVWLDRHVTTDIAKAVRDAKLHGVYVAREPRRWY
                     PGKTVGGTVIGRADIDGNGLDGIELSMNELLAGKHGKVTALRDARGHRMLADGVEETE
                     ATPGAAVKLSLDRSIQAIADDALYEAVTTHKAKSGVAVVLEVDTGRVLALSSFPMYDP
                     NTGHIPQGARNKPVTDSFEAGSVMKVFSVATALEAGVVKPETSFAIGSAFKVGPKSIT
                     DVHSYPSLTVAGIIKHSSNIGAAKIALRLGSQKLYEGYKRFGFGAKTDIELPGEQVGM
                     LRRGETWREVELATMSYGYGLTVTPLQITAALAAIGNHGIYHPPRIVDQVVDTDGTVL
                     YRGAGEARRVLSQKVADDMLPILASVFDKGPEGGTAKTFDVQGFDCGGKTGTAYKYDP
                     STRHYASDRYLASFAGLAPIANPRLAIVVQIDEPSAGEHYGGTVAGPAFAKIASESLR
                     YLGVPGAPLIPTDKWGRPLVMVDDAGRPLRDAKGSYIPKPETVKKPAPPPPAAKPEPM
                     TAPQDVEALVQTLPETPDFRGMGVSRALDEARKLHLDVEMTGTGQVVEQELMGAGKIK
                     LTFSDDARRISAP"
     gene            26726..>28365
                     /locus_tag="VMZ53_20540"
     CDS             26726..>28365
                     /locus_tag="VMZ53_20540"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HUS30916.1"
                     /translation="MTLRQLIDGLAGARLVGDANVEARAVRDDSRKVEKGDVFVAVKG
                     MRSDGHAFIPTAIERGAAAVVVEAHADPTLRIPQIVVPSTAKALGALVARSLGDPAKS
                     MKLVGITGTNGKTTTTYLVESILEAAGHKPGVIGTVSYRWGGQSHDAPYTTPTPQVLH
                     ETFAAMKEAGCTHVVMEVSSAALSMDRLSGVDFAVGAFSNLTQDHLDVHGSMEAYRDA
                     KRRLFADHLKGGVAVVNVDDVEGLGMANAGKDSIARLLRVSVEGQPADVRATFQQSTV
                     RGIAATFATPTGDVTIEAKPLIGQYNVANLALATGIAEALGISHEHIADGIAKLAGVP
                     GRVERVANAMDLDILVDYAHTPDALRNVLTALRPLTRKRLICVFGCGGDRDPTKRPKM
                     GAAVAELADLAVVTSDNPRTEDPRKILEAILPAVPKPFFVDVDRRKAIRAAIAEATPG
                     DVVVIAGKGHEDYQILGTTKIHFDDREEAAAAAAEREQRLIAGLARDASGELLGDGNG
                     VCDRYTIDSRNVLPGSLYIAIRGENHDGHRFCLDAVKAG"
BASE COUNT         4910 a         9250 c         9301 g         4904 t
ORIGIN      
        1 cctcaccgtc gcacgcggca tgatcgcgag cctcgtgttc gatgtcgcgc gtatgcgggc
       61 ggcgatcgac cagggctatc tcgtcgcgac cgaggtcgcc gattacctcg tcacgaaggg
      121 cgtcgcgttt cgcgaggcgc acgacatctc gggccacctc gtgcgcacgg ccagcgaccg
      181 tggcgtcgag ctcggcgccc tcgatctcgc cacactgaag gcggcacacg cctcgttcga
      241 gccagatctc gccgattggc tggatccggc acgcgccatc gaccggcggg acgtcgtcgg
      301 tggaccggcg cgagcgcggg tcctcgccga aatcgaccgc atcgcgagcg agttggagta
      361 gaactgccgc cccatgggtc cgttccagta tcgagatagc cagctccact gcgaggacgt
      421 ctcgctgacc aagatcgccg aagaagtcgg cacaccggtt tacgtctaca gccgcggcga
      481 catcgagcgc gcgtacaagg cgttcgatgc cgcactcgaa ggcatccccc acgaggtctg
      541 ctacgcggtc aaagcgtgct cgaccctcgg cgtgctcggc gtgctcgtcg agctcggcgc
      601 aggagccgac atcgtctcga tcggcgagct ctatcgctgg ctcaaggcag gcggcgatgc
      661 cgacagcgtc gtgttctccg gcgtcggcaa gagcgagggc gagatgaaag gagcgctcga
      721 cgccaacatc ggctgcttca acgtcgagtc cggcgaagag ctcgtcgtcc tcgatcgcgt
      781 cgcgcaggcg aacaacaagc gcgcgaagat ctcgctgcgc gtcaatcccg acgtcgatcc
      841 gcagacgcac ccgtacatct ccacgggcct caagcagaac aagtttggcg tctcgatgca
      901 ggaagcgcgc gagctgttca agagcgctgc caagatgaag ggcatcgccg ttgccggcgt
      961 cgacttccac attggctcgc agctgacgaa gacgtcgccg ttcaccgacg cgatcgctcg
     1021 cctcgtcgag ctcgtgcagc tcctcgcgaa ggacggcatc aagctcgagc acgtcgacat
     1081 cggcggcggt ctcggcatcg actacggcaa gggcgagcac gtgccgtcgc ccgccgacta
     1141 cggcaaggcc gttcgctctg cactcgctcc gctgacggat ctcggcgtga agctgctcac
     1201 cgagcccggt cgcgtgatcg tcggccaagc cggcgcgctc gtcagccgcg tgctctatcg
     1261 caagaagaac gaggcgaagc acttcacgat cgtcgacgcc gcgatgaacg acctcatgcg
     1321 cccggcgctg tacggctcgc atcatccgat caagccggtg aagaatcccg atcgtccggc
     1381 aatcgtgagc gatgtcgtcg gtccgatctg cgagaccggc gacttcttcg cgcgcgaccg
     1441 cgagctgccg gagctcgagc aaggcgagct cgtttggatc ggcgccgcag gtgcgtacgg
     1501 ctccgcgatg gcgtcgaact acaacacgcg tccgcgtgcg cctgaagtgc tcgtcagcgg
     1561 cagctcgtac accgtgatcc gaacgcgcga gacctacgag cagctcgtcg cgaacgagaa
     1621 gctcggctaa ggaaacgcga ccgcttcgct gccttcgatc ggtctcgcgt tcgtcacgtt
     1681 cgcgagccac ttctgcagct ccgccggctc gcggggtacg gacatcgaaa acagcgcgac
     1741 gaccgatgct acgggttggt gccggaaatt gaccatcccg acgtgtgtga cgctggcgac
     1801 aagggcgtgg gtcatgtcgt ggtgccctgg cagcgtgaag ctaccgccgg tctcgagatc
     1861 gaggacaacc acgactccct gcgagctcag tgtcgcgagc aggtctttgt cgatggccaa
     1921 ggcagccacc gtcggtatcg aaaagacgcg aggccacggt tgcttgccat acagcacgag
     1981 cgcgcgactg ctgtgaaata gcgcgccgtg ctgagtgatg ccgacggagt cgacaatctc
     2041 cggcgaggga agtgcgacag gcgctggagc ggcacggtcc caccgatacg cttttccgcc
     2101 ggcagcgaac cacgcggtct ccgcgtcgtc gatcgcgatg tacgtgacgg gaaacgtgac
     2161 tcgttccgtg cgacgcgtgg cgatatcgat ccgcaacagc tcgctatccc cggccagcac
     2221 atagacgcag gacttcccgg gtgctgcgat cgtcggaacc ggcgagaacg agccgaactc
     2281 tgtttctccg tttggcgtcc acagcgttac gtaacccttg cgcgtgtaca cgatacctgc
     2341 ctggccgaag ctgaagaggt cgaccttctc gatcgacacc ggcgcgcgat ccctcgcgag
     2401 gatggtcaac ccgtgatggt gcgagcgcac gaggagccag tcgtgaatcc tgtcgtacgc
     2461 gctttcgacg agcggcgtct cgatgtccaa ggctctcact cgctcatgcg tacgaagatc
     2521 gatgcgatag attccctccc ccatcgtcgt cgtccagagg tcggcgttcg agagctgcct
     2581 gggcgattcg gtgcgctcga gcgcgatcaa ctctggccgg atttcgtcga ggtcccagac
     2641 gagcacttca ccagcggtcg tcaacgcggc aatcgtgcgg ccgtcgggcg acgcgtctac
     2701 ccgtacatac gacgcatgac gcttgccgag cgccatcgtg cccatgccat cgcgaagaac
     2761 gatcgaacca tcactgtaga cggcaagaaa gccgagctgt gttcgccact gcgattgcag
     2821 ctgaaccggc tccctcgtcg cggcgcggcc gaggatggat acgccctcac cggacgtcat
     2881 gtacaggtag ttgcccgccg agaagacgcc cgtgacgagg acttgttgtg gccattcccc
     2941 gcgcttgagg agcttgccgt cgacgaggtc ccacgctgca gacatgtagc cgtgagccat
     3001 tacgacggtg gtgggtgaga tcgcgtaaat ggttgcgtac ttgtgctcgt cggctgtgag
     3061 atcgggaagg ggatacgagc ggtcacccac gacgaagttg cgcttgccgc cgcgaatgta
     3121 aatgctcgcc gagaggtccg ccgctgactc gtagcccgtc aggccctcgg cgactacgcg
     3181 tggcgtggca tcgcttgcgg tgagctcgta caacgtggtg ccggcgaaga tcctcgccag
     3241 tcccgcgcga ttcgaggcga tctcctttgc gacgaatggc caggcacggc gctggccgct
     3301 cgtgatgtcg aggaggatcg tctccgcgtc tgtggtcgcg atgaggtgcg tggcatcgaa
     3361 ccacgtaggc ttgaagatcc gggtgccgag cgcgagctcg cgggtcgacc gcgccgggag
     3421 gtcgatgagc agcagcgtcg ggctgaacaa cgacatccag aaaatgtgcc gccgatccgc
     3481 ggagacctgc atcaaccccg gttcatgcgg caggcgaaat ccgatcggga tcccggcgag
     3541 cttcgcttcc atggcaacga cccacgcatc gcgccaacgc agcgaatcgg gagccagcgt
     3601 gcggagcgcg gcgatcgctt cgagcggatc ggtagcgacg agctcgtgcg catgctggac
     3661 gagcaagcga tccgcagcgg cgcgcgcttc gcgttggcga gcttcggcga gcgtccgcgc
     3721 ttgatttgca gcatcgcgtt ctgcgacgac gcgccgaaac gaaatgatgc cgacgacggc
     3781 gagagccacg atgctgatcg ccgcgatcga agcggccgcg cgatgcttgc gcacgaaccg
     3841 cacgacgcgc gcgaaccaac cgtagttgta ggcagcaacc aggttgccga gcgtgaagcg
     3901 gcgaagatcg gcggcaagcg cagtggcgtc ggcgtagcgg tccgcgagat cccgcgacat
     3961 cgccttctcg acgatcgcgc gcagatccgc gggcacctcg gccggcatca ggcgccagtc
     4021 gggcggacgc cccgcgccga cgtgctggag catctcggtg ctgccgccat gccacgggag
     4081 ctgaccggcg agcacgtaga acagcgtcgc tccgagcgcg aacacatcag cacgcgcatc
     4141 gaccgtctca ccgcgcgctt gctccggcga catgaagccc ggcgtaccgg cgaccgttcc
     4201 tgcgcgcgtc aggtttccgt cggaaatggg ctgcgcgctc gccggctcgt cgctcgtgct
     4261 cacctcgcgc gcgagccccc aatcgatgag cagcgtctcg ccgaatggac cgacgagaat
     4321 gtttgtcggc ttgatgtcgc gatggacgat gccgtgcgag tgcgcgaatg cagcggcatc
     4381 gcaagcggcg aggatgttcg gcacgaggcg cacgcgttcg gcgaacggct tgtcggcgat
     4441 gagctcgctg agcggttcac cgtcgacgcg acgcatgatg tagtacggcg agccgtcctc
     4501 gctgcgaccc acgtcgtgca cgggcacgat tcccggatgc tcgagccgcg ctgtgatgcg
     4561 cgcttcgcgc tcgaatctcg cgaggtcaac ctcggcgtgc gagagcacat gcttgatcgc
     4621 gacttgccgg ccgagtgcac gatcgtaggc gtcgtcgacg cgtcccatgc cgccacggcc
     4681 gagctcgccg cgcgtctcga atcggtcgtt cgtgtcgaac gccgacgcgc gaggtgcttc
     4741 gagatccgtg ttcttcggag agctgttgcc gtggccagcg gccgtatccg cgctggaccg
     4801 tctctctttg acggttggct cctcgggatc ccccactcgc gatgattgta gactggtatc
     4861 ctggtggcgt tgacgtgggt accgtggctt tgcctcgcgc tgattctcgg cgcatgcggc
     4921 gataacgccg agggcgagga ccgtcaaggc ggtgacacca ccgtcgacaa tcgcagctgg
     4981 gacgcgtttc gccagccggc cgcaaatctc agcgcgggcg agcgcaaccg gttccaagcc
     5041 ggacgcagcc cgttcgactt tcgctgggac gcgccgcagc tcggcacgct gttcaacaat
     5101 gtgagctgcc tcggctgtca cgccgtgaat ggacgcggcg cttcgcagat cgggatgggg
     5161 cccgcatcgc aagcgctcgt gcgcgtcagc ctgtcgaccg gggatcctgg cgaccccggt
     5221 gggcccattc cactgccggg gtacggcacg cagctccaag atcacgcgac ggtgggactg
     5281 cccgaggttc gcgtgatgct gacgtggatc gagacgtcga tgatgtatga cgacggtgag
     5341 ctcatcgccg tgcgcgagcc gcggctcgat atcgtcggcg cgaacagcac gccgctgccg
     5401 cagggcacgc ggttttcgta tcggacggcg ccaccggtca ttggcctcgg tttactggag
     5461 gcaattccgc agtcgacgct cgaagcgctc gccgatccgg atgacgccga tctagatggc
     5521 atctccggtc gtacgaacca ggtgtgggat cccgagaagg gcgcgacggt gatgggacgg
     5581 ttcgggtgga aggcgaacac gtcgacgctg catctgcagt gcgcggccgc cgccgcaaac
     5641 gacatcggcc tctcgaacgc ggtgttcccc gagctcgatc agcagcgcga cctaaatgat
     5701 cagcagctcg aagacatggt gttcttcatg gccacggtcg gagttcctgc cgccgcaccg
     5761 cgcgatgcca aggcgctgca cgggcgcgct ctgtttcgcg acttcggttg tgcgagctgt
     5821 catgtcccga cgctcgagac cggcgatcac ccgatcgcag ccgtcgcgaa tcagcggatc
     5881 catccgtaca cggatctgtt gctgcatgac gtcggcgacc gtctcaccga tgcgcgtccc
     5941 gactttgtcg ccgaaggtgt ggagtggcgc acgccaccgc tgtggggcat tggcctcacg
     6001 aagaccgtgt ttccgatggc gacgtatctg cacgacggac gcgcgcgctc cctcgacgaa
     6061 gcgatcctct ggcatggcgg cgaagcgatg agtgcgcgtg aggcattcag aactgccaat
     6121 cgcgaggatc gcatcgcatt gatcgccttc ctcgagacgc tttgacctag aatcgaccgt
     6181 ggtggagccg gacgaaaagg gggctacgat ttccgacggt ggcccgtcgt tcgactccga
     6241 tcgcgatggt gcactgatcg gcgctgacgt cggtggctac gtcgtcgaag gcgagctcgg
     6301 ccgcggcggc atgggtgttg tctatcaagc gacgcatccg atgatcggca agcgtgtcgc
     6361 gatcaaagtg ctgaagcctt cgctctcgaa caacccggcg acggtcgagc gcttcgtgca
     6421 ggaggcgcgg tcggtcaatc agatcgggca tccgaacatt gtcgacatct tcgcgttcgg
     6481 aacgctgccg gacgggcgca gctacttgat catggatctg ctcgtcggcg aatcgctgcg
     6541 caagcgagtg aagcgtggcg cactccccgt cgaagatacg gtgcgaatcc tcgacgaggt
     6601 cgctagcgcg ctcgcggctg cgcacgacaa gggcttcgtt catcgcgatc tcaagcccga
     6661 caacgtctat ctcgtcgatc accacggtca cgtcgacgtg aagctgctcg acttcgggct
     6721 cgcgaagttg ctcgttccga gcgggctatc gggtcgcgcg ttccgtacgg cgactggcgc
     6781 gatgctcggc acgcccgatt acatgtcgcc ggagcagctg agggcgaccg gtgtcgatca
     6841 ccgaacggac atctacgcgc tcggtgtcgt cgccttcgag atgctcgcag gcgaaaggcc
     6901 gcgtcggtac agcgacggct cgttcgagct ccgcggtagc acggttccgc gctacctcag
     6961 cgaagtgcat ggcgcgccgc tcgcgcttgc cgagctcgtc gacgcgatgc tcgcgctcga
     7021 tccagagcag cggccatcca tcgtcgccat tcggacggtg ctgaagcgcg tgaagaacgg
     7081 ccaggccgaa gcggtacgcg cgattggaac ggccccatcg cttgggatct caccatcgct
     7141 tgtcggcgca cggcccgtcg ttgcgacgcc gctgtcgggc actccgacga tctccgaggt
     7201 gccgtcgcgt gccagcgctc cgtcgctacc accggtctcg caaccgccgc cgcagtacac
     7261 gtctcagcga ccgaacattg ggaggacccg gccccgtccg tccgggctcg ccaagagcgt
     7321 tcacccggcg acgaagctcg gtgtcgcacc tccgcctgaa cgggccagca agccccatcc
     7381 ggtggcggcc gctcgtcggg gcgagggcaa gctgtggctc ttcctgggca tcgggctcat
     7441 cgtgatttcg gtgatcgcac tcgtggctgt cctcgccagc tgaccgcgat acactcgcga
     7501 gaggtgaggg attcggacga cgatcggccg acggattcac atgatgtggt cggcgaggac
     7561 gacgacgaat ttcctccgga cggcgagacc gtcacgacgg tcgatgatca tctctcgccc
     7621 ggcacgaacg tcggtggcta cgtcatcgag aacgagctcg gctttggcgg catgggcgtg
     7681 gtgtacgcag cgacgcatcc ggtgatcggc aagcgcgccg cgatcaaagt gctcaagccc
     7741 gagctgtcga agacgccggc agctgtcgag cgtttcgtga tggaggcgcg cgccgtcaac
     7801 cagatcggtc atccgaacat cgtcgacatt tttgcgttcg gcgcgctccc cgacggccgc
     7861 agctactacg tgatggatct gctcgccggc gaatcgctgc gccgacgttt gaagcgcggc
     7921 ccactccacg tgagcgaagc ggtgtcgatc ctcgacgagg tcgcctcggc gctgatggcc
     7981 gcgcacgaca agggcatcat tcatcgcgac ctcaagcccg acaacatctt catggtcacg
     8041 attcccggcc gctggcccga ggccaagctc ctcgattggg gcctcttgaa gctgacgtcg
     8101 gcgcagagct ccgtgtcgag cggcaagtat cgaacgctcg ccggctcggt gatgggcacg
     8161 cccgtgtaca tgtcgcccga acaggctcgc gcgagtgacc aagtcgatct gcggaccgac
     8221 atctatgcac tcggcgtgat ggcgtacgag ctgctcgccg gtgtcgttcc gttcaagaag
     8281 ggcagctcga tcgacacgct gctcgcgcat caagacgaac cggtaccgtc gctgcaagag
     8341 aagtgtccga cgcttcccgt cgagctggtc cagctgatcg aggcaatgct cgcgaaggag
     8401 cccgatgacc gccctacgct cgcggccgtg cgcgcggtga tcaagcggct caagggcacg
     8461 aagattccga cgatgaccgc ggccggcctc gagatcacgc cgctgcccgg caagccgttc
     8521 gtcgagccaa agacgatgga gtttgtcgat cctccgacgc gccgccaaca accgcacgtg
     8581 atcgctgctc ccaagacagc agaacatgag gcggtgttcg ccgagccgcc gacgatggag
     8641 cagccgcgct cgttcgatcc aaaggcggcg ttcgtcgagc caccgacgct cgagcagccg
     8701 cgcgcgccgt caacgagggc gaacacgcga ccgacgcgcc cgcatccgag caatccattc
     8761 ggcgagactc cgacgaccgt cgcccccgca tcgttcgatc cgaaggccgc gttcgccatg
     8821 gatgccgtgg caggtctcga tgaagtgcgg cgatcttctg cggtgacgtc gccgagcatg
     8881 ccggcaccgg tgacgggcga tgtcacgtcg ccgtccctgc cgctccctgc tccaccgtcg
     8941 acgcagcacc gcatggattc gcagatgtcg tcgggcgaca gcttgccgta ccagagcatg
     9001 aacgtctcgt cgccgcagac gacgctcgca ggtcacagca tgccgatgcc gaatgcgtcg
     9061 cggccgccga tgtacacgac gccgctgccc ggctcgattc cgccgacata tcaacagcag
     9121 ccgccgatgc ggccgagtca ctcgtcgatc cctccggcaa atcagcaagc gtcgggcggc
     9181 aatcgtctgc tcgtgatcat catcgcggcc gtcatcgcgt cggcgatcgg catcacgatc
     9241 gcgctgctca cctagagctg ctggccgagc ttcgcgggcg gctgtgagcg cagatagcga
     9301 tacaacgcgc gcagatcgcc gtcggacagc gtgcggaatt cgccccacgg catcgggctc
     9361 gcggtgtcca ccgcattctt gaagcgcgcg acgaacattt cttcgctcca cgccgtgatg
     9421 tgaccggtcg ccggatccgg cgtgagattc ggtgtgacga acgtgacgcc gggcgtgcga
     9481 tgcgactcga cggtctgccc gccagcgaac gccaccccga tcgcgccgcc tgtccgcatg
     9541 tcgcgcttcg tatggcagcc accgcagttc gcgacggtgt tcgcgatgta ctcgccgtac
     9601 tccgcagtga cggccggttc gacgtgcttc ggcggcgtaa cggtgggccc cttcggctcg
     9661 aggatgaacg ccttcgcgac gcgtccgatg acattgaagt cgtgcgccgg cacgcgattg
     9721 tggatcgccg aacgactgcg caagtacgac accaccgccg ttagatcgtc gtcggcgaga
     9781 ttcgcgaacg gcatgaacgg caacatcgcg cggccggtgg ggtgcacgcc gaagcggagg
     9841 atgcgcgcga tctcgcggtc gctgtatcgg ccgatgcccg tcgcttcatc gggtgtgatg
     9901 ttgcgcgcgt agatcgtgcc gacgggtagc gaaaacttca gaccgccgcg caggtcctcg
     9961 ccgtgacacg cgacgcaatg tgcgggaccg cgaacgaggt attcaccgcg tgcgatcacg
    10021 gttggatcgc cgctggcatg aatgtccgga tacggtgcct cgaacgtgcg gttctcgcgt
    10081 gcgacgacgg cgacggcgaa tgcagtcgtg ccgaatacca ggaggagcgc gaacccgatc
    10141 gcgattctct tgaggagctt cttcatgccc cgcgaagtga tgtgacccgg tgtttcgtta
    10201 cacggattct gctacggtga gccaaatggc cgtcattgct gtatctgacg agccgtcgat
    10261 cgccgttctg gtcgcggccg cagccagggg agatcgccgc gccgaagccg ttctgtgccg
    10321 ccggttcgct ccggccgtcc gcacattttg gcggcgccgc ctgcatggcg atgcgatcga
    10381 cgaagcatcc caagacgtgc tgctccgctt cgtccaggcg cttcgcaccg gcgccgtcac
    10441 cgagcccgag cgcgtcggtg gattcgtcct cgggatctgc cgcaacctcg cacgcgaacg
    10501 cgcacgccag caagagcgcc gcgccgagct gtgggcggaa ttcggggatg cgctcgcgtc
    10561 gatcgacgag tagccgacgg tcgcctccta tcagctcgcg cagctcgaag attgtctgtc
    10621 gcagataacg aagcgatcac gcgacgtgat ccgccacgct tttatcgacg gtcactcggc
    10681 agcagagatc gccgagcgaa tgacgatgag tgaaggcaac gtgcgcgtgg tccgccatcg
    10741 cgcgctcgag tcgctgcgca cctgcatggg cgagaaggtc ttctgggagg tgccgtcgtg
    10801 agccgcctcc aggatctcga cgcatatctc acgcgcgaga tgagcgacgc agacgccgac
    10861 gcgctcgaag aagcgatgtt cgacgcgccg gacgatgcag acctcgcgtt cttcgatcga
    10921 ctgatgcgac acggccgacg cctcgtcgag cacggcacgt tcgagatggg cgtgctgcag
    10981 tcgaagatcg acgagctcat cacaaagggc cacaccgtgc aggttctcga cgcgggtcct
    11041 gccggtcagc cgcaacagac gctggtcctc tcacgcgacg cagaattcat cgtcacgaag
    11101 ctgcacctcg gtcgcaccga catcgcgcgc gtcgatgtcg agatcacgat cgtcgagcac
    11161 gacgtcacca agacgatcaa agacgtcctc gtggacccat ctgacggcat catctacggc
    11221 ctgtgcgagc ggccgctcgc gcacctcgcg ctcggggcag gcgctgcgca cacgaaggtg
    11281 cgtcgagtcg acggtgcgcg cgatgtgctc ggcgagtggc acaccgtcgg ccagctagcg
    11341 gtctagctca ctcgccggcg tcgagagcga cctgcacgcg cgcatcgttg aacgcggcct
    11401 ggccgccgag gtcggcggtc gtctgcggga tcagcgcatt cgacgtgtag ccgttgcgcg
    11461 tgtgcttgcg ccagaggcct ttgggcagga cgcagacgcc atcgcgcacg tccgtcgaga
    11521 tcttcgcgat gcagcgcact tcgccgagcg cattccacac gcgaatgttc gcgccgttcg
    11581 cgatgccacg cgcagccgcg tcggcgggcg acatctcgag cgcacccgga gccgagcgca
    11641 gctggccgaa cgtcgagctg atctgtgttg cgagcgcggg tgagatcagc gcgagcggga
    11701 actcgtcggt gcgaggatcg cgcttgtagc ggtagaggcc cttcgcctgc tgatcgagcg
    11761 cgtcgggcac gagatggatc ttcttgtcgg gcgtcgccgg gaagatgtcc gcgaatggtt
    11821 gcgggcgtgg gccgcccggc ggcgatgcca cctcgtcacg cagcagctgc gtgcgcagcg
    11881 ttgcgccttg ttcgctcgcc gcgaaggtgg cgatcaccag ctcgtcgtcg gtcatcgcgt
    11941 cgccggggcg aacgagatcg aagcggcgaa gcagctcgcc gaacagttgg ttgttgctgc
    12001 gcgcctcgcc gggcggaacc gcgacggcgg ggctgtcgaa catgcgcatc gtgccgtagc
    12061 cgcggcgcag ctcgcgatgc tcgaggaatg cagtcgcggg gaggacgacg tcggcgacct
    12121 tcgcggtgtc cgtccacact tgatcgtgga cgacgacgaa gaggtcttcg cgcttcaact
    12181 gctcgagcaa ctgatgctga gcgggcgcag tcgcggcggg attgcagttg tagacgaaca
    12241 ggcattcgat cggcggatca cgcagcgtcg cgagcgcatc ggcgatctgc cccatgttga
    12301 tcatgcgtgc ggtcgtcggt ggctcgccga tcgccgtctc cgacgacacc gtccatgcga
    12361 cgtcgctgtt ctgcatcgtg aagccgccac cgcgtgtgaa cttgcctgcg acggcgggaa
    12421 tcgcgagcac tgctgcgatc gcgctgccgc cgttgcgatt gcgctccatg ccccaacccg
    12481 cgcgaatgac ggcaggcgac gtcgcggcgt agagctcgac gaatcgatcg aggtcatcga
    12541 cgggaacctg cgcttcctgc gcggcgagct ccagcgacca ttccgaagcg cgcttcttca
    12601 gctcgtcgac ttgcagcgcg tggcgagcga ggaacgcctt gtctgcgtgg ccgcgttcga
    12661 acagcgcgtt gatgatcgac agcgcgacgg gcagatcggt gccgggccgc agcgcgagat
    12721 gtagatcggc gcggcgtgcg agcggcgtgc gccgaggatc gacgacgacg agctttgcgc
    12781 cggcctcgcg cgccttgtcg atgatcggaa cgagatggat cccggttgcg gcggggttgt
    12841 tgccccagat cacgatcagc ttcgaatgca cgaagtcttc gagcgcgacg cccggcatcg
    12901 caccgtagag accacgcgct gccgcgaccg tcgctgccgc gcagaacgtc cgatcgagat
    12961 tcgacgagcc gaagcgacgg aagaagcgtg tcgcgagcga gccttcggtg agccaaccgt
    13021 tcgagccgcc gtagtgatac ggcagcagcg ccgtcgagcc cgaggtcgca cggatctgcg
    13081 cgaggcgcgc ggcgacgtga tcgagcgctt cgtcccacgt cgcttgccgc cactgcccgc
    13141 tgcccttcgc gcccgtgcgg atcatcgggt gcagcacgcg atcgtcgccg tagagatgat
    13201 cggcgatctt gcgaaccttg ccgcagatga agccgtcggt cagcgggccg cggcgatcac
    13261 cttcgacgtc gacgacacgg ccgttatcga cggtgaccgt cagcccacac aggtcgggac
    13321 agtcgagcgg gcatgcactg cggcgctgct ccatcgacgc aatgtaaccg aatcaccagt
    13381 cgccgccgac gccaaacgtc gtcgtaccgc cctgcgtctt gctgaccgtg ggagttacgg
    13441 cgagcttggg cgcatccatg ttgcgcgtga gcacgccagc gacgagaagt cccgtgatca
    13501 tgccgccgag cgtgtagttc gtttcggagg ctgtactggt cccggccgca ttcgtctgat
    13561 ccgataggat gttctgcgtg ccgaacccga cgagcgcgcc gatcacgccg ccgacgtcga
    13621 tcagcgcagc gcggccgcgg ctgacatgga agtagcgcgt gacgaggaca ccgccgagcg
    13681 tgcccatgcc gagtcccgag aggacgagtc cggcagaaac tttctgatcc gcctcgaagc
    13741 tgccggcgaa cagcgtgccc gtcgcggtgc cccaaacggc gccggagttg atcaccgctg
    13801 cgtcaccggg cgtgatgtgc gtgcggctgc ctgcatacgc gccgatgccc gcgccgacga
    13861 tgagacccgc gccgaccatg ggcacgcgca cgttcgtctg ctccgtgaac agcgaagccg
    13921 tatcgaccgc gaccaccgcg ccgatgagcg acgacgtgat cgtgagcgag ctcgtgccga
    13981 gtggaatgtc cgagggcgtc gcgtaataac cgatgaagaa tccaccggcg agacctgcac
    14041 cgaaccccgt gagcacgagg cccgcgttct cggtcgcacc ggcgagcgtc gcgcccgcaa
    14101 ccgaaccagc gacggtcgag tacacgatca gctggcggcg accgaactgc ggtaccggcg
    14161 gcagatccat cgagattggc gtgtccttgt cggcgagcac ttcgatatcg cgcgctacgg
    14221 tgacgcggcc atttgcttcg gcaaacacgc gatacttgcc gccgggcagc gcgctctcga
    14281 atgcgcccgt gccgacgttg ccgacttgca gcttgtcgcc gttcggatac tgcaagaaca
    14341 agcgcgagtc tgcgggcacg acgcgcacgc gcagccggcc cttgagcgct tcgagcttcg
    14401 agaagatcgt gtagggcttg ccgatctcga cgtcgacatc ggtcgagacc tcctcaaaac
    14461 cctcttgatt gacgaccacg gtgtagtggc caccgacgac ggcgagctcg tcgccggaag
    14521 agccgcgcgc agcgacggtg ccttgatagt cgagcagcgt gatcttgctg cccggtggat
    14581 cggtattgac gcggaccttc gcgcgcaggt tgcgaagcac tttgatgcgc gccgagacgt
    14641 tctctttttc gatctgcgga tcattgccgc actgccccgt tcgctttgcg tcgggcggca
    14701 cggcgaggac gtacttctcg aaatacgcga tcgccttctc gtactcgagc tcgcgctcgt
    14761 acgcctggcc gatgtccttg aggaccgcgt agtacggcgc gaagcagtac gcggagacaa
    14821 gctcggtgac cgctccgtga taatcgccct gaacgtagag aacctcgccg cgatcgaagt
    14881 gctcggcggc cgcacctcgc agctgatcgg gtgtcatctc gtcgtgctcc gggcatcgat
    14941 cgatctccag cacctgcgtg ccgccgatga agccgcgatc ttcgccgcgc acatcgatgc
    15001 gcttgcccgg tgtgaagtcg tccggcaccg gcttgctgtc gtcgggacgt tgctgcgcac
    15061 gagcgctccc cagcgcagcg caaacggcga cggcagacgc gagccagacc ctcacttgtt
    15121 ctggactgta ccgctacccg cgtacggatt gttgctgagc tcgcccgcag tgtggttcgt
    15181 cgtaccggtc tgcgtcgtgc ccgtcgaggc ggagcccttg cctgtcggcc ggatgatctt
    15241 cgcgccgctg ccgctctgct tcttgagctt gatcgggacg gtcgcgccgt tcttgcgctc
    15301 ggcgggattg atcttcgtct gccccacgaa gccgttcggc agctcggcgt agatgttgac
    15361 gatgtcgttg ttcttcacga gctcgatctc caccggcgct ttgccgacga gctcggactc
    15421 gcgatagatg tctgccttcg gcgggtcgct cgtgatcgtc agcttgatcg tctcgaacgc
    15481 gggcttcgct tcacccgcat cgatcacagg gagtgcctgc acgccggcat cggagtgcaa
    15541 cgcgacctgt ggctgtggca cttgcttctt ctgcttgctg cccatgagca acacgacggc
    15601 gatcgtcaca gcaccgacgg cgatgcccgc gaggctgatc gcccacgcga tgcgaggaat
    15661 cttctgcacc tgcttcggca gcaggcgacg cggcccggtg atcttcgcgc gctccgtcga
    15721 gtgcgtcgga tcgtcgagca gcgcgttcaa gtcggccagc atgtccgtcg cgttcgcgta
    15781 gcgatcgttg cgatctttcg cgagcgcctt gctcacgatc gcttcgaact cgtcggagag
    15841 ctcggggcgg atttcgcgca cgggcttcgg agtctcgttg agcacctgcg agatcaccga
    15901 gaggtagttg ttgccgccga acggcacgcg tcccgtcgcg gactcgtaca tgatcacgcc
    15961 gagcgcgtag atgtcgacgc gatgatcgag atcttcgtcg ccacgcgcct gctcgggaga
    16021 catgtacagc ggcgtgccga gcaccatgcc cgtctgcgtg agccgcaccg cttcttcttc
    16081 ttcgcctgac gcacgcagcg acttcgagat gccgaagtcg acgaccttga cgaagtccat
    16141 ctccttgcgc ttcagcagga agatgttctc gggcttgatg tcgcgatgga cgacgccctt
    16201 cgcatgcgcg gccgccaatg ccgatgcggt ctgcgtggcg atgcgaagga tgcgcgcttc
    16261 ggggagcttc ggctctttgg cgaggcactc ggccatcgac tcgccctcga ggaactccat
    16321 cacgacgaac gttcgtccgt cgtccgtgtt gccgaagtcg gtgatgtcga tgatgtgctc
    16381 gttgccgatc gacgacgcga gctgcgcctc ttgcttcaag cgcttcacga tcgcttcgcg
    16441 ctgcgcgtac ttctcgagca agaccttgac cgcaacgcgc ttgccgatca gcgtgtgcgt
    16501 tgcctcgtag accgcgccca tgccgccctg cccgaccttg cgcgtgacga ggtagcggcc
    16561 cgacagcgtc acgccggtca gctccgtcgc cgacggctgg agcgcttgca tcccggccgc
    16621 tggtgtcgga cccgcttccg tcgcgctcag cgacttctcg gacgagtcgt tcatgccgtg
    16681 gctgctcttg gtcgcgaccg caactgccgt atcgctcgcg cccatcgacg tgtcgctcgc
    16741 tccatcgagc gaacgcacga ccagtcgtcc cggcgtatcg gctgctggcc ggccaggcga
    16801 cgacccacca ccgggcttat cgccagaacc cgtagacacc tacgatctcg atggtaggtg
    16861 gcccactacg ccaagtcaaa ccgcgggctt gctctcaccg agccgaaacg attgaaaatt
    16921 gccgacatct gcggacatcc atcgaccact tgccttcatt cgaataatgg aatacaagaa
    16981 tgatcgtgct ggccgcgcgt gcagacctgt tccggctcct cggcgacgag gatcggctcc
    17041 gcctgctcgc tctctgcgcc gaagaggagc tgaccgtcgg cgagctggca acgctgctcg
    17101 gcgagagcca gcctcaagtc acaaaaaaga cccagccgct ccgcgaattc ggcctgctca
    17161 gcgcgcgcaa agacggcact cgcacgctgc tcaagaccga cgtcgtcgag gcccgctccg
    17221 ataagggacg gacgcaactt ccgcaacccg ctggtctgac cactaaggga cggacgcaac
    17281 ttccgcaacc tgctgtccga aattcggaca ccgatggtct cgcggatgcg ctcgatcccg
    17341 tgatcgcggc ggcgctcgaa gaaggccgtg cgctctgcgc gaaggatgga tcgctcgcaa
    17401 ggatcccgcg cgtgatcgcc gcgcgcgaag aagtttcgcg gcgcctgttc gatcagcccc
    17461 aggacgaacg cccagcaccc gcggtcgatc cgacgctgct gtcgtggctg ccactgctcg
    17521 cacctctctt gccggggcgc gcgcttgcgg tcgacatcgg cactggcgaa ggcgcgctcc
    17581 tgccgctctt gtcgccgcta ttcgatcgcg tgatcgccgt cgatcgcagc gctgcgcgat
    17641 tggcgcgtgc ggcgtcgaac atcgcgagct gggatcttcc caacgtgcgg ttgcgcgaag
    17701 gcgccgtcga cgatgctgcg ctcgccgagg aagtgtaccg gttcacccgc accgacgatc
    17761 cgcgcggacc gcgaggcgcc gaccttgtcg tcatggcgcg cgtgctcgta catgcgtcgc
    17821 gcccacagga tgtcatcgcg caagcggcgc agctcgtgcg acctggcggt catctcatcc
    17881 tgatcgatta cgttccgcac gacgacgagt cgctgcgcga gcaaggtcac gtctggctcg
    17941 ggttcgagcc cggcaagctg cgcacgtggc tcgaggctgc gcggctccag accgtcgctg
    18001 ttcaaccgtt ttcacaacct ctcttgcaac tagcagtcgg cagaaaaccc ggagaccacc
    18061 atggacactc gtaacgaatt tcccgcgttc aagatcaaag acctcacgtt ggccgagctc
    18121 ggccgcaaga agatccgcat ggccgaaaag gaaatgccgg gtctcatgtc gctgcgcgag
    18181 gagtacaagg gcaagtcgcc gctcaagggc gcgcgcgtcg ccggctgcct gcacatgacg
    18241 atcgagacgg ccgtcctgat cgagacgctc gtctcgctcg gcgcggaagt cacctggacg
    18301 tcgtgcaaca tctactcgac gcaggacgaa gctgccgcgg cgatcgcgaa ggcgggcgtt
    18361 ccggtgttcg cgtggaaggg cgagaacctc gaggactact ggcagtgcat cgagatgcag
    18421 ctgacggcgt tccagggcgg caagggcccg aacctcatcc tcgacgacgg cggcgatctg
    18481 acgctgctcg ttcacaaggg cgtcgaagcc gagaagaagg gcagcgcgcc cgatccgaag
    18541 acggcgacga acgaagagat gcgcgtcatc tacagcgtgc tcacggagtc gctgaagaag
    18601 agcaaggatc gcttcacgac gatggcgaag gagatcaagg gcatctccga ggagaccact
    18661 acgggcgtgc accgtctcta cgagatgggc aagaccggct cgctcctgtt cccggcgatc
    18721 aacgtcaacg actcggtgac gaagtcgaag ttcgacaacc tctacggctg ccgcgagtcg
    18781 ctgttcgacg gcatcaagcg cgcgacggac gtgatggttg cgggcaaggt cgtcgtggtc
    18841 gccggctacg gtgacgtcgg caagggctgc gcgcatgcag ctcgcggcct cggcgcacgc
    18901 gtgctcatca ccgagatcga tccgatctgc gcgctgcagg cggcgatgga aggctacgaa
    18961 gttgtcacga tggaagaggc ggctccgatg gccgacatct tcgtgacggc gactggctgc
    19021 tgcgacgtga ttcgcgccga gcacttccag gtgatgaagg acgaggcgat cctctccaac
    19081 atcggccact tcgacagcga gattcaggtc gcgtggctcg agaacaacaa agagatcaag
    19141 gaagagaaca tcaagccgca ggttgatcag ttcatcttcc cgaacggcaa gcgcatcacg
    19201 ctgctcgctc gtggtcgcct cgtgaacctc ggctgcgcga cgggtcaccc gtcgttcgtc
    19261 atgtcgaact cgttcacgaa ccagacgatc gctcagctcg cgctgtggac gaacgcgcag
    19321 aagggcaagg acgagatgac cggcatgaag ttcgaagccg gccaggtcta cgtgctgccg
    19381 aagaagctcg acgagaaggt tgcgcgcctc cacctcaaga aggtcggtgt gaagctgacg
    19441 acgctcacga aggagcagtc ggactacctc ggcgttgcgg tcgacggccc gttcaagccc
    19501 gaccactacc gctactagcg agcggccgct ggcgcgaagc tacgcagcac gcccttcgcg
    19561 ccacgcggtg atcttgtcga tgaacattcc gatcggaagc tcgcgcttcg gatcgatcgc
    19621 gatggtcggg ttcgagagta cctgcgtgaa gctggcgctc ttgagcttac ccttcggcgt
    19681 cttgtagccg agctcgagcg tcatgagggg gcccttcgag cccttcgcga cggactggat
    19741 ctcgttgacc gtcacggcga gcgcgacctt gccttcggtc gggttcgtga agatgaggcg
    19801 ctgcgtcgtc agaacaagct ttccgtcgct cgggctgccc gcgacgatga ggccgccgaa
    19861 gcgatggccc ggccagttcg cgtagatgta ggcgatgcgc gtttcgccgg gcgctagttt
    19921 gagcttcccg ccgcgcaacg accagtacac gccgaagccg atgaggcccg tgatcacgat
    19981 gccgacgatc tgaccaatct tgtccgcgtt gccgtccata cgttgtcctt atcccgagca
    20041 actctgcgtc acgcacgtgc tcgtattgaa gtcgcagttg ccgctctcgc actgcagcga
    20101 cgtcgagcac gccgagccgt cgggcttcgc ggccacgcac ttcagagtcg cgccatcgca
    20161 gtagctcttc tcgtcgaagc agcggctgtt gctgctagcg cacgcttcgc cgagcgacgg
    20221 tggcctcttg cacatcgtcg tcgcgaagtc gcacgggaag tacggcgagc agtcggtgct
    20281 cgacgagcag gtcgcgccga cgagcccgag cgctttgcag accgcgcctc cacagtgcaa
    20341 tccctcgtcg cgacacacgc cgtcgggaca cgcctcgttc ggtcccggca gcgtcttgca
    20401 cgtgcgcggc gctcctgcac atccgagccc gaaaccacac tcgcgcgtat ccacgcagtc
    20461 agcgccggcg atcttcagcg gcgtgcacac gtcggtgctc gagtcgcaat acgagccgcg
    20521 gccgcacccg acgatgcggt tgcacggctc gccgttgatc ggcagctcca gcgacggtgg
    20581 cgtgtcaccg ttgcacgcgc ccatctcgca gaggccatcg gcgacgagcg cacggcagtc
    20641 ttgcgagatg cactccgcgt ccgtcagaca cggcgcatct gcggcgagcg ttccgcgcgt
    20701 gaacgtgtag caagccgaga tcggggcgcg cccaatctcg tcggtcttgt cgcacgtcgc
    20761 attcgcgatc gcatcgaagc acgcgtgcac ggcattgccg ttgtagagca cgcggcccgc
    20821 ggcaatctcg gctcgcacgt ccggcgagag cgagtacgcg ccacccgcga acagcgctgc
    20881 gctcgtgcag gtcgcctgat cggggaacaa cccgcagcgc acgaggtacg tgcactcggc
    20941 gtctttgtac gcggtgtcga agtctgcgac cgagacgtac tcacccgagc cgctgtcgtc
    21001 gtcaccacac gcgatcaata gaagtgcgac tgccgccacc ctacgcatgc ggcgatcata
    21061 tcagtcatcc gcgcggcggt tctttttgat ctcggcgcgg tggcgtttgc cctcgacgcg
    21121 ccggcgcttc gaggagcggg tgggcttcgt cgcgaagcgc ttcttcgggc gatcgagcgc
    21181 ggcgcgaacg atcagcgcaa gcttggacat cgcgtcgttg cgattcttga tctgatcgcg
    21241 cgtaagcgtc gacgtgacga tcaggtcgcc ttccgtcgtg agcttgttcg cgagccgcga
    21301 caccagatac gagcgatccg cgtcggcgac tgccgtcgac gtcgtcgggt tccagcgcag
    21361 ctcgaccttg ctggcgacct tgttgacgtt ctggccaccc ggaccgcccg agcgcgcgaa
    21421 cgcgatccca agctcgccgg cgggaatcgt cagccgaggc gtgacgacga tgtcgtgcat
    21481 gacactgctc gcctgcccgt ctgcttacgc tgcgccggac ttcttcgagc gcttcttcgg
    21541 cggcgccttc tgcgcttcct catagacgcg gagctcggtc tcggcggtcg tctgcacggc
    21601 ctcgagatac agcgaggtca gcgccatgtg gtcaccgcgc agcgcctcgt agtagcgctg
    21661 gcgatcgatc gaatggatga tcgcgatcgg gtaaccggct tcctgcagga gctggttcga
    21721 cgcgatgcgg atcgtgcgac cgctctcctt cgcccacggg aagatcgcca tgaacttgga
    21781 atgcagcgac gcgacgcgct cgatcacatg cgtgtgcttc gacgtcggat cgtcgatcca
    21841 ctcgccgagc ttgcgcatcg ccgggaggat tttctcgggc ggcgcgatgt cgtgatagta
    21901 gaggcggtgg agcgggttct ccttgcggaa cgggagcccc ttcgccttct cgtccggtga
    21961 caagatcgaa tagatctcgc gaaccgtatc gagcttgaac ggcttcttct tgttgccggc
    22021 gttctcggcc gcgaagttac acgcgtcatc gaagcggcga atttcttcgt agctcgggat
    22081 gagcgatgga tcgctgatga tgctcggatc aaccgcggcc ttgatctcgc tgtagctcag
    22141 gacctcgcct tcgagcgcgg cgtcatgatg aatgagcgaa atgcgcagcc ggttgttgaa
    22201 ctctgtgcgc gcgacatcgt tcgcggtggt cagcgcttgc cgccacgcgg tgacggtgcg
    22261 ttcgacctcg ctgaagcgag cgtcgatgtc ctcggtttct ttgtacctga gcacagttat
    22321 ccctatccgc gaagtcggtg gagtctacga aacgccatcc ggatcgtcaa gctaaccgag
    22381 tgtgacttcc tactacctaa ctgcacacga gttgcgaatc aaccgcaaga tttctgcaat
    22441 gttgcgacat ctgcgtggta ctcaggccac gtgtccgccg ccgggtcaag cgggtccgac
    22501 ttcgcgcacg aatcggtgct gcttgccgag accatcgcga acctatcccc gcgaaacgac
    22561 gagatttacg tcgactgtac cctcggcgga gccggccact cctcggcaat cctggccgcg
    22621 gccccaaacg cccggctgat cggtatcgat cgagaccctg ccgcgctggc cgccgcacgc
    22681 acgcggctgg aaagtggtct taccactaag ggacggacgc aacttccgca acccggcggt
    22741 cagaccaccc tggtccacgg cgagatgggc gacatccgat cgatcctcgc ggacctcggc
    22801 atcacccagg tccacggctt tctggcggac ctcggcgtgt catcgccgca gctcgatcac
    22861 gcagaccgcg gcttctcgtt ctccaaagaa ggcccgctcg acatgcggat ggatccgacg
    22921 cgcggcccga ccgctcgcga cctactgcgc gccacgagcg aagtggatct cgcgtcgctc
    22981 atcagcgacc tcggcgaaga gcgccacgcg agacgcatcg cacgcacgat caaagaggcg
    23041 ctccgcgaaa acgaagacgc gctgcgcacg acgaccgagc tcgccgcgct cgtcacgaaa
    23101 tgcatcccga tcgtcgagca gcgaaagtcg aagatccacc ccgcgacgcg gacgttccag
    23161 ggccttcgca tcgcggtgaa tgccgagctc cagcagctcg agcggttcct cgacgcattt
    23221 cccgatcttc tcgcgccgga cggtcgctgc gtcgtaatca gctttcactc tctcgaagat
    23281 cgtctcgtga agaacgcgtt tcgcgatctc gcgtggtcca catcgctacc gcccaagctc
    23341 gcgcagcaag cgggcgagcg gctcgagccg gtcgtcgagg tgctcacgcg caaagccgtg
    23401 ttcgccagcg acgaggaaac cgcgcgcaac ccacgcgctc gctccgcgcg ccttcgcgca
    23461 tgtcgccgaa cggcggcgcc gaacgtgcca atgggcggct ccgccggcga cccgcacctc
    23521 cgcggccgcg actagtccgc ctgccggact gctccgtccc tcgtggttgc ggaggttgcg
    23581 gaagttgcgt ccgtccctta gtggtcaggc cactgctccg tccctcgtgg ttgcggaggt
    23641 tgcggaagtt gcgtccgtcc cttacgggaa tacccgggtc attgggggcg caggcagcgg
    23701 cggattgcga cgtcgcggag gaacacgatg ccgtggaatt cgagatcgag atcgcggacg
    23761 cgcggatcga tcgcctggac ttgtgtgcag tctgcggagc gcgcggcggc agcggcttgc
    23821 tttgtgagct cccacgcttc ggcgcgatgg cgctcgcgac gtgtgaggta gggggcggcg
    23881 tgctcgttcg ctttgtcgtc cgtcgcgatg aagacgagcg cgccgatgat cgagattggg
    23941 atgaacgcga gaccgacggt gaggatctgg gtgtcgtgcg cgggaatcgc tgaggcggtg
    24001 acgcccgcgg cgagcattcc gacgaggcca ccggcggcag cgacttcacc ggcgcgatgc
    24061 gcgcgtcgtg cttggggtgt ggtgcatccg gacgtgctca cgagcgacag cgtagcgagc
    24121 agcaggaggg acagccggcg catccgacaa gtatggggac aatttcccgt cgacggcgag
    24181 gcgaaaaaat tgcccgaaca acgctgcggg gttgtagtgg agcgacgtga catgacgtcg
    24241 cctggggccg cttcggtcta tcgcattttc aagagatcgg acggagcagc atccgtccga
    24301 tcggtcgtca ttgcgatggc gctcgtcgcg tgtgtgctga cggggcttgg tgtcgtgagg
    24361 gtgacgcggc agcatgaagt gctcgcgctc ggctatcagc tggatcgaga ggccgagcat
    24421 ctgcgtcagc agaaagagat caatcggcgg ctgcaagtcg agctcgcgac gcagtcgtcg
    24481 cctgatcgtg ttcggcggct cgctgcgcag ctcggcatga cgcccgtgcc gccggatcgc
    24541 attcgcatca tcgacgtgcc gagtcgcaag aaggtcgcag cagctccatg acccgcgaca
    24601 agaagctaaa gccgatgacg cccggcgtgt cgtcgtcggc gcgcgttcgc gcgtacctcg
    24661 caggcgcggt cgtgtcgctc ggactgttcg gcgtcgcgat gaaagcgtgg gcgctgcagg
    24721 tcgacgacgg tgcgaagtat cgcgaggcgg ctgcacgcca gcatgcgatg cggctggaca
    24781 ttcccgcgcc gcgcggcgag gtcagcgatc gcatgggcag gccgctcgcg attacggctg
    24841 atgcagacag cgtgtgggcg aatcctcgcg aggttcacga cgtggcgggg accgcggaga
    24901 agctcgcagc gttgctgcat ggtgatgcag gcgtgctcga gtcgaggctc gcgggcgatc
    24961 gcggcttcgt gtggctcgat cgccacgtga cgaccgatat cgcgaaggca gtccgcgacg
    25021 cgaagctgca tggcgtctac gtcgcgcgcg agccgcgccg ctggtatccc ggcaagacgg
    25081 tcggcggcac ggtgatcggt cgcgcggata tcgatggcaa cggtctcgac gggatcgagc
    25141 tctcgatgaa cgagctcctc gccggtaagc acggcaaagt gacagcgctg cgcgatgcac
    25201 gtggccaccg catgctcgcc gacggtgtcg aagaaaccga agcgacgcct ggcgccgccg
    25261 tgaaactctc gctcgatcgc agcattcaag cgatcgccga cgacgcgctc tacgaagcag
    25321 tcacgacgca caaagcgaag agcggcgtcg cggtcgtact cgaggtcgac acgggccgtg
    25381 tcctcgcgct gtcgagcttt ccgatgtacg acccgaacac gggccacatt ccgcaaggcg
    25441 cgcgcaacaa gccggtgacg gactccttcg aggccggctc cgtcatgaag gtgttctccg
    25501 tcgcgacagc gctcgaggcc ggcgtcgtga agccagagac ctcgtttgcg atcggctcgg
    25561 ccttcaaggt cggaccgaag tcgatcaccg acgtgcactc gtatccgtcg ctgaccgtcg
    25621 ccggcatcat caagcactcg agcaacatcg gcgccgcgaa gatcgcgttg cgactgggct
    25681 cgcaaaagct ctacgagggc tacaagcgtt tcggctttgg cgcgaagacg gacatcgagt
    25741 tacccggcga gcaggttggc atgcttcggc gcggcgagac gtggcgcgag gtcgagctcg
    25801 cgacgatgtc gtatggctac ggcctcaccg tgacgccgct gcagatcacc gcagcactcg
    25861 ccgcgatcgg caaccacggc atctatcatc caccgcgcat cgtcgatcag gtcgtcgata
    25921 ccgacggcac cgtgctgtat cgcggcgcgg gcgaagctcg ccgtgtgctg tcgcagaagg
    25981 tcgccgatga catgctgccg atcctggcga gcgtcttcga taaagggccc gagggcggca
    26041 ccgcgaagac gttcgacgtg cagggcttcg actgcggcgg caagacgggc accgcgtaca
    26101 agtacgatcc gtccacgcgg cattacgcgt cggatcgcta cctcgcatcg ttcgccgggc
    26161 tcgcgcccat cgcgaatccg cgtctcgcga tcgtcgtaca gatcgacgaa ccctccgccg
    26221 gcgagcacta cggcggcacc gttgccggtc cggcattcgc gaagatcgcg agcgagtcgt
    26281 tacgctatct cggcgtgccc ggcgcaccgc tgatcccgac ggacaagtgg ggccgcccgc
    26341 tcgtgatggt cgacgacgcc ggcagaccat tgcgcgacgc gaagggcagc tacatcccca
    26401 agcccgagac ggtgaagaag ccggcgccgc cgccgcccgc cgcgaagccg gagccaatga
    26461 ccgcaccgca ggacgtcgag gcgctcgtcc agaccctgcc ggagactccg gacttccgcg
    26521 gcatgggtgt gtcgcgagcg ctcgacgaag cgcgcaagtt gcacctcgat gtcgagatga
    26581 cgggcaccgg gcaagtggtc gaacaggagc tgatgggcgc tggcaagatc aagctgacgt
    26641 tctccgacga cgctcggcga atttccgcgc cctaagtgcg cttgattcac tgcacgaaat
    26701 cgctgaagct gcgcgttcta tcggcatgac gctccgccag ctcatcgacg gtctcgcagg
    26761 cgctcgcctc gtcggcgatg cgaacgtcga agcgcgcgct gtgcgtgacg attcacgcaa
    26821 ggtcgagaag ggcgatgtgt tcgtcgcggt gaaaggaatg cgcagcgacg gccacgcgtt
    26881 tattccgacc gcgatcgaac gcggagctgc ggcggtggtc gtcgaggcac atgccgatcc
    26941 gacgctgcgc attccgcaga tcgtcgtgcc gtcgaccgcc aaggcactcg gtgcgctcgt
    27001 cgcgcgctcg ctcggggatc cggcgaagag catgaagctc gtcggcatca ccggcacgaa
    27061 cggcaagacg accacgacgt acctcgtcga gagcatcctc gaggcagccg gtcacaagcc
    27121 gggcgtcatc ggcaccgtgt cgtatcgctg gggcggccag agccacgacg cgccatacac
    27181 gacgccgacg ccgcaggttc tgcacgagac gttcgcggcg atgaaagagg cgggctgcac
    27241 gcacgtcgtg atggaagtgt cgtcggccgc gctgtcgatg gatcggctct cgggtgtcga
    27301 cttcgccgtc ggtgcgttct cgaacctcac ccaagatcac ctcgacgtgc acggctcgat
    27361 ggaggcgtat cgcgatgcga agcgccggct gttcgccgat catctgaaag gcggcgtcgc
    27421 agtcgtcaac gtcgacgatg tcgaaggcct cggcatggcg aatgcgggca aggattcgat
    27481 cgcccgtctg ttgcgcgtgt ccgtcgaagg tcaacccgcc gatgttcgcg cgacgttcca
    27541 gcagtcgacg gtgcgcggca tcgcagcgac gttcgcgact ccgacgggcg acgtgacgat
    27601 cgaggccaag ccgctcatcg gtcagtacaa cgtcgcgaac ctcgcactcg cgaccggcat
    27661 cgcagaagcg ctcggcattt cgcacgagca catcgccgac ggcatcgcga agctcgccgg
    27721 tgtccctggc cgtgtcgagc gcgtcgcgaa cgcgatggac ctcgacatcc tcgtcgatta
    27781 cgcgcacacg cccgacgccc ttcgcaatgt gctcaccgcg ctgcgcccac tcacgcgcaa
    27841 gcgcctcatc tgcgtgttcg gctgcggcgg cgatcgcgat ccgacgaagc gaccgaagat
    27901 gggcgctgcg gtcgcggagc tcgccgacct cgccgtcgtg acgagcgaca acccgcgcac
    27961 cgaagatccg cgcaagatcc tcgaggcgat cctgcccgct gtcccgaagc cgttcttcgt
    28021 cgatgtcgat cgacgcaagg cgatccgcgc cgcgatcgcc gaggccacgc cgggcgacgt
    28081 cgtcgtgatc gcgggcaagg gccacgagga ctaccagatc ctcggcacga cgaagatcca
    28141 cttcgacgat cgcgaagaag cggcagctgc agcggccgaa cgcgagcagc ggttgatcgc
    28201 aggccttgct cgcgacgcga gtggcgaatt gctcggcgac ggcaacggcg tctgcgatcg
    28261 ctacacgatc gatagccgga acgtgctccc gggttcgcta tacatcgcga tccgtggcga
    28321 gaaccacgac ggccatcgct tctgcctcga tgccgtcaaa gccgg
//
LOCUS       DATLBZ010000407         7251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1238644, whole genome shotgun sequence.
ACCESSION   DATLBZ010000407 DATLBZ010000000
VERSION     DATLBZ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238644"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2843)
                     /locus_tag="VMZ53_20545"
     CDS             complement(<1..2843)
                     /locus_tag="VMZ53_20545"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30917.1"
                     /translation="MSSSDDPLLAVMRDIARAPAIELSTLAEPLRAGCVIGRFELVRE
                     IGRGGFGVVWEAVDSELGRRVALKTLATARLSSGGIDALKREARVVAKLAHPNLVTLH
                     DVIVHDGSPVLVLELLEGETLEARLVRGALAIPALVDVALAIARGLARAHADGVAHLD
                     LKPSNVFCTTQGTTKILDFGLAAIANMPGHRAGTAGYMAPEQWRGTPDARSDLFALGV
                     VLYRGVSRELPFAAGDLRNLDRAPPALDPELAPVELSALVASLLQTDPARRPANADEV
                     VSSLEQIAERLRADRDPTRNPYRYLDAFTEADRRWFCGRSREIAMLGRLVEQKPLVAV
                     VGTSGAGKSSLVHAGLAASVAARGWRVIRLRPGDSLEMLDRPIDGAALVVVDQLEEVF
                     TRIQDASSRRLFLEAVLSRARDDVRVVVSIREDFLTRLSELPALRDAVAGGMMLLGPP
                     DREALVETLRGPARTLGVEIADDVVAAVIGGVEKELAPLPLVQVAASRLWELRDRTHG
                     RIDATALAKLGGVGGVLATHAEEVVRELRGAGELEVARNIFRLLVTAGRTRIARRHAD
                     LIAAAAGPSAERVLGVLIAGRLLAVTTRDGVDEVELAHESLITGWTRLATWLDEDSEA
                     LRWRDRLRQAATLWKERGRPSELLWKGSTLAEARGALAGTRDDFLDASTLKSVRARRL
                     RRGLLALAAATLIVTTIMAVVSVRESRRAAAASHREALVQRAAAAKDPLIAALILREL
                     PAAQWPESAVPVALEVSQRAIPMARVVLGTIATTVLSSPRGDVVAVGGDKGHLLRWSS
                     TTQLTTEDLGSPITALAENANHSRIAMSLEDGRVMLTSFTADTVNEVARTDPFNTIAW
                     GDGWVVGITDAGKVVQIAVETHTVKTLDGPPANPEVAIAVSRDGKRIAYGSEGNVVVV
                     SGTTTRTFTGHTDVVYAVAFIDRDRIASASADRT"
     gene            complement(2840..3421)
                     /locus_tag="VMZ53_20550"
     CDS             complement(2840..3421)
                     /locus_tag="VMZ53_20550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS30918.1"
                     /translation="MTAEALDNAVDEHVAAGRFEAAAVLAIRELGPQILGYLAALVGD
                     FERAREVFSVFSEDLWSGLSSYRAEGTFRAWAYRVARNAALRTMRDPYWRRGRRLESE
                     EHSELAAAVRSTTQAHARPSADAKLARLRSALTPDEHALLILRVDRDLSWSEIASALT
                     TGRSRPDEATLRKRFERLKEKLRRLAEAEGMLR"
     gene            complement(3425..4384)
                     /locus_tag="VMZ53_20555"
     CDS             complement(3425..4384)
                     /locus_tag="VMZ53_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS30919.1"
                     /translation="MVATWRTLALAVALSLIPAVVHADAPWSQGVSEEQKAKAKVALD
                     QGNALLIDKKYVEALDKYTEAVAQWDHPAIRFNMVRCQIQLGKNLEAYENLEKALKYG
                     ADPLEETVYNEALAYQKLLQTQIGDVQVTCKQDGVKLTIDGQPLATCPAQESRRLLAG
                     PHQIVGSKPGLLPKTMDVVVIGGQPLGVDVKLDPLAKGARIVHRWPGFLPWTVFGGGL
                     ALGAVGGVSLWAASNQIKSYDAFIDANCTGQCTDADLMEARRDYYDPAQTKKKIGGTM
                     VVVGVASVATGALMIYLNRGRTVYPDVAPLPGGGGAVSLSGTF"
     gene            complement(4413..5315)
                     /locus_tag="VMZ53_20560"
     CDS             complement(4413..5315)
                     /locus_tag="VMZ53_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30920.1"
                     /translation="MSKSNLLALLFASSSLIACADEAPQPDLLALPGSAYFPESLNAG
                     ADGTLYVGSLATGQVVAFDDGATAPRTIIEAGAHGVTGVTGVLVEGDTLWLGSVDTTF
                     QRPTEIRSFSLTGEPKATYPLAASYFVNDLAFDKHGNLFATDSFSGTVQRLKAGASAF
                     ETYVQDARLAPATQGAFGLDGIVVANDAIYVNKLDTSQLFRISENGAVTEITVTPPLV
                     GPDGMRGLDNDTLLVIDGGANRLSKVEIHGTTATSAPLSSDLDQPTAVAISRGSAWVS
                     EGQLGRLFTGQNATLPFSVRRVAL"
     gene            5382..6308
                     /locus_tag="VMZ53_20565"
     CDS             5382..6308
                     /locus_tag="VMZ53_20565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUS30921.1"
                     /translation="MKHPDLNLLIALDALLQEQNVSRAAKRLGLSTPAMSHALARLRK
                     QLGDPILVRAGQRMVVTPRAADLRDRVHALSVETLGVLRPEPSRDLKNLERAFRIKAS
                     DMVLCVLGPALDKMVRELPGVSLHVMPPQFEEPALLRAGTVDLAIGVYDYSPYSDLPS
                     DLRIQQLFEDHFTCVVRKDHPTVGRTLTIDEYVALDHVQIAPRGQPGGYVDELLAERG
                     HKRRIVRAVPYFIAALVLVAETDYVLTVSGQLAKRLAEPLGLRLVQPPRSLDLEPNHM
                     AQLWHPRNDKDPGHRWLRETIAEAAKLSTRNR"
     gene            6353..>7251
                     /locus_tag="VMZ53_20570"
     CDS             6353..>7251
                     /locus_tag="VMZ53_20570"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HUS30922.1"
                     /translation="MVHPQSARLIETIRQSIIGDDSALEGPYGPRRVTYADYTASGRS
                     ITFIEDFIRNEVMPLYANTHTETSGTGLQTTTFREDARAIIHRAVGGGPDDCVIFCGS
                     GATGAINKLLGVMNLCIPADLDARHKLVDKIPAAERPVVFIGPFEHHSNELPWRESIA
                     DVVVIHEDADGHVDLAHLEEELVRYAARPLKIGSFSAASNVTGIATDTALVSTLLHKH
                     GALSFWDYAAAGPYVQIAMKEDAALAYKDAVFISPHKFIGGPGTPGVLVGKRALFHNR
                     VPVVPGGGTVRYVNLEEHSYLEDP"
BASE COUNT         1243 a         2431 c         2348 g         1229 t
ORIGIN      
        1 gtgcgatcgg cgctcgcgct ggcgatacgg tcgcgatcga tgaaggcgac cgcgtacacg
       61 acgtcggtgt gaccggtgaa cgttcgcgtt gtcgttccac ttactacgac gacgtttcct
      121 tcagacccat atgcgatgcg ctttccatcg cgcgacaccg caatcgcgac ctctggattg
      181 gccggtggtc cgtcgagcgt cttcaccgtg tgcgtctcga ctgcaatctg aacgaccttg
      241 ccggcgtccg tgatcccgac gacccaacca tcgccccacg cgatggtgtt gaacggatct
      301 gtccgcgcga cctcgttcac agtgtccgcg gtgaatgacg tcagcatcac gcggccgtcc
      361 tcgagggaca tcgcaatccg ggaatggttc gcattttcgg cgagcgccgt gatcgggcta
      421 cctaggtctt ccgttgttag ctgtgtcgtc gagctccagc gcagcaagtg tcctttgtca
      481 cctccgacgg cgaccacatc tccgcgcgga ctcgagagga ctgtggtcgc gatcgtcccg
      541 agcaccacgc gcgccattgg aatggctctc tgcgacactt cgagcgcgac gggaacggcg
      601 gactccggcc actgcgccgc gggaagctcg cgaaggatca gcgcagcaat caaaggatcc
      661 ttcgcggcag ctgcacgctg cacgagcgct tcacggtgcg aggctgcggc tgcacggcga
      721 gactcccgca cggagactac cgccatgatt gtcgtcacga tcagcgttgc ggcggcgaga
      781 gcgaggagac cgcggcgcag acgccgagct ctcacgctct tgagtgtcga ggcgtctaga
      841 aagtcgtccc gcgtgccggc gagcgcaccg cgcgcttccg cgagcgtcga gcccttccat
      901 agcagctcgg aaggtcgacc gcgctccttc cacagcgtag cggcttgacg cagtcgatcc
      961 cgccagcgca acgcttcgct gtcttcgtcg agccacgtcg ccaaacgggt ccaaccggtg
     1021 atcagcgact cgtgtgccag ctcgacctca tccacaccat ctcgcgtggt cacggcgagc
     1081 aagcgacccg cgatgagcac tccgagcacg cgttctgcgc taggtcccgc ggcggcggcg
     1141 atgaggtcgg catgccggcg cgcgatccgg gtgcgacccg cggtgacgag caatcgaaag
     1201 atgtttcgag cgacctcgag ctcaccggca ccgcgcagct cgcgcacgac ttcttcggcg
     1261 tgagtcgcga gcacaccgcc gacaccaccg agtttcgcga gtgcggtggc gtcgatgcgt
     1321 ccatgcgttc gatcgcggag ctcccagagg cgacttgcag ccacttgcac gagcggtagt
     1381 ggggcgagct ccttctcgac accgccgatc acggccgcta cgacgtcgtc tgcgatctcg
     1441 acgccgagcg tgcgcgcggg gcctcgaagt gtctcgacga gcgcttcgcg atccggcgga
     1501 ccgagaagca tcatgccgcc ggcgacggcg tctcgcaggg ccggcagctc cgacaagcgg
     1561 gtgaggaaat cttcgcgtat cgacacgacg acccgaacgt cgtcgcgcgc acgagacagc
     1621 accgcttcga ggaacagccg gcgcgagctg gcgtcctgaa tccgcgtgaa cacttcttcg
     1681 agctggtcga ccacgacgag cgcggctcca tcgatcggcc gatcgagcat ctctagcgag
     1741 tcgcccgggc gaagtcggat gacgcgccag ccgcgtgccg cgacggacgc cgcgagccct
     1801 gcatgcacga gcgagctctt cccagcgcca ctcgtgccga cgaccgcaac gagcggcttt
     1861 tgctcgacga gccggccgag catggcgatt tcgcgggacc gtccgcagaa ccaacgccgg
     1921 tccgcttccg tgaacgcatc gagataccgg tacggattgc gcgttggatc ccggtcggct
     1981 cgaagacgtt cggcaatctg ctcgagcgaa ctgacgacct cgtctgcgtt cgccggacgc
     2041 ctcgccggat ccgtctgcag caacgacgcg acgagtgcac tcagctctac aggtgcgagc
     2101 tcgggatcga gcgctggtgg tgcacgatcg agattgcgca agtcgcccgc tgcgaacggc
     2161 agctcacgac tgacaccgcg atagaggacg accccgagcg cgaacaggtc actgcgcgcg
     2221 tcgggtgtgc cgcgccattg ttcgggagcc atatacccag cggtgccagc gcgatgccca
     2281 ggcatgttcg cgatggctgc caagccgaag tcgaggatct tcgtcgtgcc ctgcgtcgta
     2341 cagaacacgt tgctcggctt gaggtcgagg tgcgcgacgc catccgcatg cgcgcgcgca
     2401 agcccacgcg cgatcgcgag cgcaacatcg acgagcgccg gtatggccag cgcgccacga
     2461 acgagcctcg cctcgagagt ctcaccttcg agaagctcga gaaccaggac cggcgagccg
     2521 tcgtgaacga tcacgtcatg cagcgtcacg agatttggat gtgccagctt tgcgacgacg
     2581 cgcgcttccc gcttgagtgc gtcgattccg ccggaagata agcgtgctgt cgcgagcgtc
     2641 ttcaatgcga cgcgacgacc gagctcgcta tcaaccgcct cccacacgac gccaaagccg
     2701 cctcggccga tctcgcggac aagctcgaac cgcccgatca cgcatcccgc tcgcagcggc
     2761 tccgcgagcg tcgacagctc gatcgcaggt gctcgcgcga tgtcgcgcat gaccgcgagc
     2821 agcggatcgt cgctcgagct caccgcagca ttccctcggc ctcggcaagc cggcgcagct
     2881 tctccttgag ccgctcgaac cgcttacgca gcgttgcttc gtcgggacgg ctcctgcccg
     2941 ttgtcagtgc gctcgcgatt tcgctccatg aaaggtcgcg atcgactcgc aatatcagga
     3001 gagcgtgctc gtctggtgtc agcgcgctgc gcaagcgcgc gagctttgca tccgcggacg
     3061 ggcgtgcatg ggcttgcgta gtcgagcgca ccgccgccgc gagctcggaa tgttcctcgc
     3121 tctcgagtcg ccggccacgt ctccagtatg ggtcgcgcat cgtccgcagc gctgcgttgc
     3181 gcgcgactcg gtacgcccac gctcgaaacg tgccttccgc gcgataactc gacagtcccg
     3241 accacaggtc ttcggagaac acagaaaaca cctcgcgcgc tcgttcgaag tctccgacga
     3301 gcgcagcgag gtacccgagg atctgcggcc cgagctcgcg gatcgcaagc accgcagcag
     3361 cctcgaagcg ccccgcagcg acgtgctcgt ccacagcgtt atcgagcgct tctgcggtca
     3421 cgcctcagaa ggtgccagaa agcgagaccg caccgccacc accgggcagc ggcgcgacgt
     3481 cgggatacac ggtgcggccg cgattgagat agatcatcaa ggcgcccgtc gcgacggagg
     3541 cgacgccgac gacgaccatc gtcccgccga tcttcttctt ggtctgcgca ggatcgtagt
     3601 agtcgcgcct cgcctccatg agatctgcat cggtgcattg gcccgtgcag ttcgcgtcga
     3661 tgaaagcgtc gtacgacttg atctggttgc ttgccgccca cagcgagacg ccgcctacgg
     3721 cgccgagcgc gagaccgccg ccgaataccg tccatggcaa aaagcccggc caacgatgga
     3781 cgatccgcgc gcccttcgcg aggggatcga gcttcacgtc gacgccgagc ggttgaccgc
     3841 cgatgaccac gacatccatc gtcttgggca gtaggcccgg cttgctgccg acgatctggt
     3901 gcggcccggc gagcagtcga cgcgactctt gcgcgggaca cgtcgcgagc ggctggccat
     3961 cgatcgtcag cttgacgccg tcttgcttgc acgtgacttg gacgtcgccg atctgcgttt
     4021 ggagcagttt ctggtacgcg agcgcctcgt tgtagacggt ctcttcgagc ggatcggcgc
     4081 cgtacttcag cgccttctcg aggttctcgt acgcttcgag gttcttgccg agctggatct
     4141 ggcagcgcac catgttgaag cgaatcgctg ggtgatccca ctgcgcgacg gcctcggtgt
     4201 acttgtcgag cgcctcgaca tacttcttgt cgatgagcag cgcgttgcct tggtcgagcg
     4261 cgactttggc tttcgctttt tgctcttcgc tgacgccttg cgaccagggc gcgtcggcgt
     4321 gaacgactgc ggggatcagt gacagcgcga ctgcgagagc aagcgttcgc cacgtagcga
     4381 ccatcgcccc cagtatcgat cagatccggg cgctagaggg cgactcgacg gaccgagaac
     4441 gggagagttg cgttctgacc tgtaaacaaa cgcccgagtt gtccctcact cacccacgcg
     4501 gagccgcgcg agatggcgac ggccgttggc tggtcgaggt cgctcgagag cggcgccgag
     4561 gtggccgtcg tgccgtgaat ctcgaccttc gagaggcggt tcgcgccgcc gtcgatgacg
     4621 agcagcgtgt cgttgtcgag accgcgcatg ccatcggggc cgacgagcgg cggcgtgacc
     4681 gtgatctcgg tgaccgcgcc gttctcgctg atccggaaca gctggctcgt gtcgagcttg
     4741 ttcacgtaga tcgcgtcgtt tgcgacgacg atgccgtcga gaccgaacgc gccttgggtg
     4801 gccggggcga gccgcgcgtc ttggacgtac gtctcgaacg cggaggctcc ggccttcagg
     4861 cgctgcaccg tgccgctgaa cgagtcggtc gcgaacaggt tgccgtgctt gtcgaacgcg
     4921 aggtcgttca cgaagtagct cgccgcgagc gggtaggtcg ctttgggctc gccggtgagc
     4981 gagaagctgc ggatctcggt cggacgctgg aacgtcgtgt cgaccgagcc gagccagagc
     5041 gtgtcgccct cgacgagcac acccgtgacg cccgtgacgc cgtgagcacc ggcctcgatg
     5101 atcgtgcgcg gcgccgtggc gccatcgtcg aacgcgacga cttgtccggt cgcgaggctg
     5161 ccgacgtaga gcgtgccgtc ggcacctgcg ttcaggctct ccgggaagta cgccgagcca
     5221 ggcagggcga gcaggtcagg ttgcggcgcc tcgtcggcgc aagcgattag cgaggaggag
     5281 gcgaagagca gcgcgaggag gtttgatttc gacatgccgc caacatcgag ccggcaggtg
     5341 tccgtcgcaa tgcacgcaga tgaacactat agttgcacga aatgaaacat ccagacctga
     5401 acctcttgat cgcgctcgat gcgctgctcc aggagcagaa cgtgtcgagg gccgccaagc
     5461 ggctcgggct ctcgactccc gcgatgagcc acgcgctcgc gcgcctgcgc aagcagctcg
     5521 gcgatccgat cctcgtgcgt gctggacaac gcatggtcgt cacgccgcgt gcggcggatc
     5581 tgcgcgaccg cgtgcatgcg ctctccgtgg agacgctcgg cgtgctgcgg ccggagccgt
     5641 cgcgcgacct gaagaacctc gagcgcgcgt tccggatcaa agcgtcggac atggtgctgt
     5701 gcgtgctcgg gcccgcactc gacaagatgg tgcgcgagct gcctggcgtc tcgcttcacg
     5761 tgatgccgcc gcagttcgaa gagcctgcgc tgctgcgcgc tggcaccgtc gatctcgcga
     5821 tcggcgtcta cgactactcg ccgtactcgg atttgccgag cgacctgcgc atccagcagc
     5881 tgttcgaaga tcacttcacg tgcgtcgtgc gcaaagatca cccgacggtc ggacgcacgc
     5941 tcacgatcga cgagtacgtc gcgctcgatc acgtgcagat cgcgcctcgc ggccagccgg
     6001 gcgggtacgt cgacgagctg ctcgccgagc gcggccacaa acgccgcatc gtgcgcgcgg
     6061 tcccgtattt catcgcggcg ctcgtgctcg tcgccgagac cgactatgtg ctcacggtgt
     6121 ccgggcagct cgcgaagcgc ctcgccgagc cactcggctt gcgcctcgtt cagccgccgc
     6181 gctcgctcga cctcgagcca aaccacatgg ctcagctgtg gcacccgcga aacgacaagg
     6241 atcccggcca tcgctggctg cgcgagacga tcgcggaagc ggccaagttg tcgacgagaa
     6301 accgctgacg tagcgcgtcg aagactccgt cagataaccg tgttatgtcg gcatggtgca
     6361 tccgcaaagc gcgcgtctga tcgaaacgat tcgccagtcg atcatcggcg acgacagcgc
     6421 gctcgaaggc ccgtacggcc cacgccgcgt cacgtacgcc gactacaccg cgagcggccg
     6481 ctcgatcacg ttcatcgagg acttcatccg caacgaagtc atgccgctct acgcgaacac
     6541 gcacaccgaa acgagcggca cgggcctgca gaccacgacg tttcgcgagg acgcgcgcgc
     6601 gatcattcat cgtgcggtcg gcggcggccc cgatgactgc gtcatcttct gcggctcggg
     6661 cgcgaccggt gcgatcaaca agctcctcgg cgtgatgaac ctgtgcatcc cggccgatct
     6721 cgatgcgcgt cacaagctcg tcgacaagat ccccgcggcg gagcggccgg tcgtgttcat
     6781 cggccccttc gagcatcact cgaacgagct gccgtggcgc gagtcgatcg ctgatgtcgt
     6841 cgtgatccac gaagacgccg acggtcacgt cgatctcgcg cacctcgaag aagagctcgt
     6901 gcgctacgca gcgcgaccgc tcaagatcgg cagcttctcc gcggcgtcga acgtgacggg
     6961 catcgcgacg gacacggcgc tcgtctcgac gctgctgcac aagcacggcg cgctgtcgtt
     7021 ctgggactac gccgccgcgg gaccgtacgt gcagatcgcg atgaaagagg acgcggcgct
     7081 cgcctacaaa gatgccgtct tcatctcgcc gcacaagttc atcggtggcc ctggcacgcc
     7141 gggcgtcctg gtggggaagc gcgcgctctt tcacaatcgc gtgccggtgg ttccgggtgg
     7201 cggcacggtg cgctacgtga acctcgaaga gcacagctac ctcgaagatc c
//
LOCUS       DATLBZ010000408        12174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1241021, whole genome shotgun sequence.
ACCESSION   DATLBZ010000408 DATLBZ010000000
VERSION     DATLBZ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241021"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VMZ53_20575"
     CDS             <1..549
                     /locus_tag="VMZ53_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30923.1"
                     /translation="SVTRVFHNQIFENNTVNFAPSGNIVGLVPTGTGMAILAAHQVEI
                     FDNDIRDHKSINIGVISYVPTGKSFTDASYDQYPTGISIHDNRISGTSDMPTGMLGAL
                     LISAIGEIHPNPPFIVPDLAWDGVLDPMREYTAADKICISGNGDANFINLAWPLNMGD
                     APSEDMTPHACTLPALGGVDLP"
     gene            546..1643
                     /locus_tag="VMZ53_20580"
     CDS             546..1643
                     /locus_tag="VMZ53_20580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HUS30924.1"
                     /translation="MKRAVVLAALAACGGDGDDTCALDGAMCKHLSSWSLFDDIGAQQ
                     PAPGVIPYDLNTPLFSDYASKDRFVRLPEGQQARWTTDGVIDLPVGSVLVKTFSYLHD
                     RRDASQGRTLVETRVLVHGETGWHGASYLYGSSTDDAQLAIAGAIVDTSWIHDDGAAR
                     TNHYVVPNQNQCKNCHAEHDSVVTPIGPKARHVQPERLQALVDAGALADAPPRDQWPA
                     PIVSLDYAGHAASGTLDERARQWLDINCAYCHNPKGAARTSGLFLNWDETDMAKVGLC
                     KSPVATGRGSGGLQYDIVPGQPDQSILVYRMSSTEADVRMPELGRNLVHEEGVALVRE
                     WITAMTGDCSASARTGWTGSSGDADSHLSTR"
     gene            1728..2528
                     /locus_tag="VMZ53_20585"
     CDS             1728..2528
                     /locus_tag="VMZ53_20585"
                     /inference="COORDINATES: protein motif:HMM:NF039689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6209 family protein"
                     /protein_id="HUS30925.1"
                     /translation="MATLTAGAVACAADSSTDGTGGGGGKADGSTPTLTFGKDWSEKV
                     TGTLLAGSPVRINYALDRLQECRGSTNGSEVWGASGYASFDGKAPVTFGVSQLVGDVV
                     KPSAAELEIPAGTHSVELWFASNNIWGCIAYDSNMNANYQFDVDASSTGAVLSFDSDF
                     SETQSDAIHAGDSIVVHYDPSRLAQCQYSSGGHAGWSITAHYQVDGGAVKTLPVTRAE
                     GSTLVAADPTLAVPRGHDLALWFSATSAYGCHAYDSNMNANYHFAIEN"
     gene            complement(2525..3565)
                     /locus_tag="VMZ53_20590"
     CDS             complement(2525..3565)
                     /locus_tag="VMZ53_20590"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HUS30926.1"
                     /translation="MSPGDDDGLAVDASVSPSDGDDGEATDATAATPHGYIGAYKSPT
                     IVRDGSTYHAYFAADRVAGSHRAVPHATFTADGNWHFEGDALPHLGAQAVDNEFVWAP
                     AVAKISADRWMLYYTAHLAGTVSKMCIWRAHATSPDGPFVDDYAGPLVCPNGTQWAID
                     AYVVKDGHGNWNLAVRYDLPGGINTIQLRELGPQGGAFAPGSEWSELVRNSPSSWEQP
                     VLENAGIVRLTPPNGAAHWFVFYSGGAWDDNSYAIGYADCGPSIKGPCTKKTPNGPWL
                     ATDPSRKLYGPGTPTFYTSQSGKMLMSIQAWEFSGGKANDKNAKGQIMRTYAISVGDN
                     YKPHVTLVRVDR"
     gene            3780..4643
                     /locus_tag="VMZ53_20595"
     CDS             3780..4643
                     /locus_tag="VMZ53_20595"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HUS30927.1"
                     /translation="MRRLMFLLVALSACTASVEEDLDPAGTPDDTTDDGPPADDPGDQ
                     MTTPDPQEPPETINGVPLFQVPFPCGQVWAGQTRTNHSPAPSVDFNRLDDDGDPVVAA
                     ASGVVARVENLGSSSYGRWIEINHGNGYRTRYAHLSAQLVAVGQTVKRGQQIGNVGTT
                     GGSTGPHLHYEQRHNNVTERASFNGVMALYYGSKNYTSQNACNVAGVVGRVNTGGAVL
                     TVYADSTTASASVSSVANGATVTITCQKIGQSVSGTYGTSTLWDKIGTGFVPDAYVST
                     GSDGQVAPTCP"
     gene            4838..6808
                     /locus_tag="VMZ53_20600"
     CDS             4838..6808
                     /locus_tag="VMZ53_20600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS30928.1"
                     /translation="MSQQQLGKYIVLRRLGAGGMAEVFLCKLQGIGGFEKHVVLKKIR
                     DDAANDADFVTMFFDEARLAANLNHPNIVQTFEVDALDGMPYIAMEYVKGATLAQVLR
                     KMKQEYLKPRYGHFASIFAGVCAGLDYAHNAKDGSGRSMQIVHRDISPQNIIVSLDGI
                     PKIFDFGIAKARGSLALTGVDRVKGKFAYMAPEQLRAKPVDSKADVFAIGVCLYEAMT
                     GKRPFGGGTEGELIARRLTGEFVTPSEVVHKFPAELEQIILDAMAPDPTARPSALELH
                     QRLAAFASAAPFQSSSIAVGAWMREMFDDDSSAYDSIESSPSMTPVPASLPTGATPRP
                     SASTVSVDRATPPPAHTPRRGRWIVGVLAAATALSATAAGIKLVRHDDHAASPAPAPI
                     VATAPPPAPAPIVAPQPAPPPPAAVAPPSEVPAAAVAAPAPDAPPPEEVVTVERKPGP
                     TKIVRRPTVKPKATSTKAAPVAQGSATISETKPVAPAGSAPSTPAITVPPPTGSATTT
                     ITITTTAPQSVTVNKPPPTGPTATTAPAIAVPTPASPGSLDAVPSIVRVSVDGSLTTS
                     EVQTALGRTLDALRACYRTAAKQAKSTPDLTVKVLFEIDEGARASRVRLSGDTLGLSS
                     CVSASVGSLRTRVAPDVGTVSVTATIRFKPTR"
     gene            6845..8170
                     /locus_tag="VMZ53_20605"
     CDS             6845..8170
                     /locus_tag="VMZ53_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30929.1"
                     /translation="MSAVLVLAAPVVWRVSAAQPQPDPLAPGASVPTAPVPDAGVQPD
                     APTEVPVTPAPATPAITAPLEAVAPEPDPEPEPEPDHKPQMHRNFAGSIQLDYMSVAT
                     EKTGRDVALDGATAEVSMKLAMDFSSKISANVKMCVACHGVEVGMAYFDIRLADEMNV
                     RVGRFTPSFGEFPVRHDPANHRTSDKPLPYDMGRMLRITEWNEGVLPAPWVDNGIEIN
                     GTHFFGEHVQTDYAVYAIGGPRAGSDPVDFDFKRSRSGESYYIDNNSRPAIGGQLSAS
                     IATDTTTTSIGASMMRGTYDPEHRLPFAIYGAHAVFRVRDVFLRAEYLWRRTKMSLGD
                     DPMERFRYAPGSTGMFDPYFVKDGAYAELEIPAGPRVTLIWREDGMRRRGNVVSTSDM
                     RSDSAIVRHTAGIAIAIRSALRLKASYEYYDFSDYEDESVLHLGIAGPF"
     gene            8179..9234
                     /locus_tag="VMZ53_20610"
     CDS             8179..9234
                     /locus_tag="VMZ53_20610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30930.1"
                     /translation="MKSTRLLYIVTLAGCDMLGPRTSDIAIDAPPSSHHDAAVDASTY
                     LLPAGSDVPRITTNAELTSQIKINDGLSDSALAAAGGVITRSTGKASGATVMYWNFGA
                     APLAENFVASAPLYVLCDDDGNGNLTPRTGHPYLIDSIPGDPRYSPIRRIINVPVTAS
                     YNGQLITSVEALAEAYDRGLVGEPKPGGTWRDMPVVPTGTLLEVSSTVAPAAATEVYG
                     RGFVVELFPLGGALGIQPLRNGVIPIGQESRLLSGVATGMPPVMPTALDPQPVFQYGI
                     PAAPPTTTFNWTPIVTELDVRLASGVDPTTVTNDASLFKRSTTGAITGYYTTTVDNYT
                     VMTTVSNKPIQFVDGAP"
     gene            9231..9698
                     /locus_tag="VMZ53_20615"
     CDS             9231..9698
                     /locus_tag="VMZ53_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HUS30931.1"
                     /translation="MKSSLLLVAVAACDITYVPDVGPLQNEPAADVDGGTAGMNQPLA
                     RCIDSDPSTDVSFSLQIRPLTTRSPGGCLSCHGTNTTSGFNLGSYESLRRGGLNSGTR
                     IIVAGDPCASILPQKLGVAPPYGARMPYNGPPYFTPAELTLVRDWIAEGAVDN"
     gene            9718..9984
                     /locus_tag="VMZ53_20620"
     CDS             9718..9984
                     /locus_tag="VMZ53_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30932.1"
                     /translation="MVLVAGCGEPLSRTSLVASPETSPDVRRACAVTEQKCTRCHTLG
                     RVLAADATTRDQWEPIVLRMRRMASSGISSNDADVVLHCLASRR"
     gene            complement(9986..10690)
                     /locus_tag="VMZ53_20625"
     CDS             complement(9986..10690)
                     /locus_tag="VMZ53_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30933.1"
                     /translation="MTFTRSAIFSLCVVGAAVGCKRDRNQSDETSTYQRTNEDTTTTT
                     APGHTVPNGTTVGGETGGMGTAGRADTATNPGSQEGTANDTASGTANSGMSNSGTGTS
                     GTAANTDVDTTGTGVTAGGSVNGTSDTSGTSGTSGTITDQNRAARRGTARGTAGGTNS
                     GTGTGTGTGANSNQGTGTTGGMDDSGANDNGTAGPSGTSTNGTNGTNSTGTSGTSGTS
                     GTSGTSGTSGNAPNSQ"
     gene            complement(10732..12024)
                     /locus_tag="VMZ53_20630"
     CDS             complement(10732..12024)
                     /locus_tag="VMZ53_20630"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30934.1"
                     /translation="MRRAALLLVLAACGDNLAGTGSFELVGHTDLGGRGMNSAIAIAG
                     DTAYVGSRIDGKPILIVDIADPANPTVVGEIGTPLEGLPGMSSRELRVVGNTLVVMNL
                     QCSPSLHGCATSAGEVENLRFFDITDPRAPVVRASYPVQGTSQMFPRSPHEMFLWRDP
                     ADASRSLIYLAAPGVPSLEVIDVATATNVLRWDPYAQGGVPKGGANNILHSIAISDDG
                     KSAYLSHQVAGLFVVDLTSFPTLTTLTPAANAPKFGAMGPHSAVPVPGRSLLVTTEEV
                     YPPPFGAGCPWGHLRMVDIADPTRPMLLGEYKLAENDPACAADGPMIAFTSHNATVTH
                     DLAIVTWYAGGLQAVDISDPANPTQLAEFRPEPLAAVAVEDPGLGGSHVEMWSYPIIK
                     DGLIYVVDVRNGLYVMRYRGRWADQIRTTPFAEGNSNL"
BASE COUNT         2121 a         4113 c         3882 g         2058 t
ORIGIN      
        1 tcggtcacgc gcgtgttcca caatcagatc ttcgagaaca acaccgtgaa cttcgcgccg
       61 agcggcaaca tcgtcgggct cgtgccgacg ggcaccggca tggcgatcct cgccgcgcac
      121 caagtcgaga tcttcgacaa cgacatccgc gatcacaagt cgatcaacat cggcgtgatc
      181 agctacgtgc cgaccggcaa gtcattcacg gacgcgagct acgaccagta cccgaccgga
      241 atttcgatcc acgacaaccg catctcgggc acgagcgata tgccgaccgg catgctcggc
      301 gcgctgctca tctcggcgat cggcgagatt catccgaacc cgcccttcat cgtgccggac
      361 ctcgcgtggg acggcgtgct cgacccgatg cgcgagtaca ccgccgccga caagatctgt
      421 atctccggca acggcgacgc gaacttcatc aacctcgcgt ggccgctcaa catgggcgac
      481 gcgccgtcgg aagacatgac accgcacgcg tgcacgttgc ctgcgctcgg cggagtcgac
      541 ctgccgtgaa gcgagccgtc gtccttgctg cgctggccgc atgcggcggc gatggcgacg
      601 atacgtgcgc gctcgacggc gcgatgtgca agcacctgtc gagctggagc ttgttcgacg
      661 acatcggcgc gcagcagcct gcgcccggcg tgattcccta cgatctcaat acgccgctgt
      721 tctccgacta cgcatcgaag gatcgcttcg tccgtttgcc cgaaggtcag caggcgcgct
      781 ggaccaccga cggtgtgatc gatcttcccg tcggcagcgt gctcgtcaag acgttcagct
      841 atctccacga tcgccgcgac gcctcgcagg gccgcaccct cgtcgagacg cgcgtgctcg
      901 tccacggcga gaccggctgg catggggcgt cgtatctgta cggcagctcg accgacgacg
      961 cgcagcttgc gatcgcgggc gcgatcgtcg acacgagctg gatccacgac gatggcgccg
     1021 cgcgcacgaa ccactacgtc gtgccgaatc aaaaccagtg caagaactgt cacgccgagc
     1081 acgacagcgt cgtgacaccg atcggtccga aggcgcgaca tgttcagccc gagcggctgc
     1141 aagcactcgt cgatgcaggc gcgctcgcgg atgcaccgcc tcgcgatcag tggccggctc
     1201 cgatcgtgtc actcgactac gcgggacatg cagcgagcgg aacactcgac gaacgcgcgc
     1261 ggcagtggct cgatatcaac tgcgcgtact gccacaaccc gaaaggcgcg gcacgcacgt
     1321 cgggcttgtt cctgaactgg gacgagacgg acatggcgaa ggtcggcctc tgcaagtcgc
     1381 cggtcgcgac gggccgcggc tcgggcggac tgcaatacga catcgttccc ggccagcccg
     1441 accaatcgat cctcgtctat cgcatgagct cgacggaagc cgacgtccgc atgcccgagc
     1501 tcggccgcaa cctcgtccac gaggaaggcg tcgcgctcgt ccgcgaatgg atcacggcga
     1561 tgacaggaga ctgcagtgcg tccgcgcgca ctggctggac cggtagtagt ggcgacgcgg
     1621 atagccacct ctcgacgcgg taggtcatcg agatcacgca cgccgtcggt ggcgcgggct
     1681 tcgcacaacg ggcccgcatg attcgcagta cctacgcttc gttgcttttg gcaactctga
     1741 cggctggcgc ggttgcctgt gccgcggact cctcgacgga cgggacgggc ggcggcggcg
     1801 ggaaggccga tggctccacg ccgacgctca cgttcggcaa ggactggagc gagaaggtca
     1861 ccggcacgct gctcgccggc tcgccggttc ggatcaacta cgcgctcgat cgcctgcaag
     1921 agtgccgcgg ctcgacgaac ggcagcgaag tctggggcgc atcgggctac gcgagcttcg
     1981 acggcaaggc gccggtcacg ttcggcgtgt cgcagctcgt cggcgacgtc gtgaagcctt
     2041 ccgccgcgga gctcgagatt cccgccggca cgcacagcgt cgagctctgg ttcgcgagca
     2101 acaacatctg gggttgcatc gcctacgact cgaacatgaa cgcgaactat cagttcgacg
     2161 tcgatgcgag ctcgaccggc gcggtcctct cgttcgactc cgacttcagc gagacgcaat
     2221 ccgacgcgat ccatgcaggc gactcgatcg tcgtccacta cgatccgtcg cgcctcgcgc
     2281 agtgccagta ctcgagcggc ggccacgcag gctggagcat cacggcgcac taccaagtcg
     2341 acggcggtgc cgtgaagacg ctgcccgtca cgcgcgctga aggctcgacg ctcgtcgcgg
     2401 ccgatccgac gctcgcggtg ccgcgcggtc acgacctcgc gctgtggttc tcggcgacga
     2461 gcgcgtacgg ctgccatgcg tacgactcga acatgaatgc gaactatcac ttcgcgatcg
     2521 agaactagcg atcgacgcgc accagcgtga cgtgcggctt gtagttgtcg ccgacgctga
     2581 ttgcatacgt gcgcatgatc tggcccttgg cgttcttgtc gttcgccttg ccgccggaga
     2641 actcccacgc ttggatgctc atgagcatct tgccgctctg tgacgtgtag aacgtcggcg
     2701 tgccgggacc gtagagcttc cgagatggat cggttgcgag ccacggtccg tttggcgtct
     2761 tcttcgtgca cgggcctttg atcgacgggc cgcagtccgc atagccaatc gcgtacgagt
     2821 tgtcgtccca cgcgccaccc gagtagaaga cgaaccagtg cgcggcgccg ttgggcggcg
     2881 tgagccgcac gatgcctgcg ttctcgagca cgggctgctc ccacgacgac ggcgagttgc
     2941 ggacgagctc gctccactca gagcccggcg cgaatgcccc gccctgcggc ccgagctcgc
     3001 gaagctggat cgtgttgatg ccgcctggca agtcgtagcg gacagcgagg ttccagttgc
     3061 cgtgtccgtc tttgacgacg tacgcatcga tcgcccactg cgttccattc gggcacacga
     3121 gcggtccggc gtagtcgtcg acgaacggac catcggggct cgtcgcgtgc gcgcgccaga
     3181 tgcacatctt cgagacggtg ccggcgagat gcgcggtgta gtagagcatc cagcggtcgg
     3241 cggagatctt cgcgactgcg ggcgcccata cgaattcatt gtcgacggcc tgcgcgccga
     3301 ggtgcggcaa cgcgtcgcct tcgaagtgcc agttgccatc tgcggtaaac gtcgcgtgcg
     3361 gaacagcgcg atgcgagccg gcaacgcgat cagcagcgaa gtacgcgtga tacgtcgagc
     3421 cgtcgcgcac gatcgtcggt gacttgtacg cgccgatgta gccgtgcggt gtggccgccg
     3481 tcgcgtcggt cgcttcgccg tcatcgccat cgctcggcga gacgctcgca tcgacagcga
     3541 ggccgtcatc gtctcccggc gacaaaggtg aggcgcatgc agcaacgaga accgcgaggc
     3601 tcgaaaacaa ggatcggctg tacatgcgcg acacctcgca cgaaaaaatg cttcacacca
     3661 gccaacggtt gtttgcgggg tctacccctc accgcgccac gccgtcctca atgcagcgtg
     3721 ctcagaatgc gattccaccg cggtagcgcg cggcatggtc gttgcctata gcgccgtcga
     3781 tgcgccgctt gatgttcctg ctcgttgctc tgtccgcatg cactgcgtcc gtcgaagagg
     3841 atctcgatcc tgccggcacc cccgacgaca cgaccgacga cggaccgccc gccgatgatc
     3901 ccggcgatca gatgacgacg cccgatccgc aggaaccgcc cgagacgatc aatggcgtgc
     3961 cgctgttcca ggtcccgttt ccgtgcggcc aagtctgggc cggccagacg cgcacgaatc
     4021 acagccccgc gccgtctgtg gacttcaatc gtctcgatga tgacggcgat ccggtcgtcg
     4081 ccgcagcgag cggcgtcgtc gcgcgcgtcg agaatctcgg aagctccagc tacgggcggt
     4141 ggatcgagat caaccacggc aacggctatc gcacgcgcta cgcacatctc tccgcccagc
     4201 tcgtagccgt cggccaaacg gtgaagcgcg gccagcagat cggcaacgtc ggcacgaccg
     4261 gcggatcgac gggaccgcac ctgcattacg agcagcggca caacaacgtc accgagcgcg
     4321 cgtcgttcaa cggcgtgatg gcgctctatt acggttccaa gaactacacg agccagaacg
     4381 catgcaacgt cgccggcgtc gtcgggcgcg tgaacacggg tggcgcggtg ctgacggtct
     4441 acgccgattc gacgacggcg agcgcgagcg tcagctccgt cgcgaacggc gcgaccgtaa
     4501 cgatcacgtg ccagaagatc ggccagtccg tcagcggcac gtatggaacg tcgacgctat
     4561 gggacaagat cggcacgggc tttgtgcccg acgcgtacgt ctcgacggga agtgacggcc
     4621 aagtcgctcc cacgtgcccg tagcaacatt ttgttgcacc tctctcgacg acagaatcgt
     4681 ggtctcgcta acacgcttcg atcgcgttga gctcaaagcg atcgagacgc gtcgcgaagc
     4741 ttacgaatcg cgtgtgatgg attcgttgta ggcgcgatct cacatcgaca agtgagagcc
     4801 gcttcgctga gatgcttctt gcgcaggtgg cgcatgaatg tcccagcagc aacttggcaa
     4861 gtacatcgtg cttcgtcggt tgggggcggg tggaatggca gaagtgtttc tctgcaagct
     4921 gcaaggaatc ggcggcttcg agaaacacgt cgtgctcaag aagattcgtg acgacgcggc
     4981 gaacgacgcc gacttcgtaa cgatgttctt cgacgaggct cggctcgccg cgaacctcaa
     5041 ccatccgaac atcgtccaga cgttcgaggt cgatgcgctc gacggcatgc cgtacatcgc
     5101 gatggagtac gtcaagggcg cgacgctcgc gcaggtgctc cgcaagatga agcaggagta
     5161 cctcaagccg aggtacggcc acttcgcgtc gatcttcgcc ggcgtgtgtg cgggcctcga
     5221 ctacgcgcac aacgcgaagg atggctccgg gcgatcgatg cagatcgtgc atcgcgacat
     5281 cagcccgcag aacatcatcg tctcgctcga cggcatcccg aagatcttcg acttcggcat
     5341 cgcgaaagcg cgcggcagtc tcgcgctgac cggcgtcgat cgcgtgaagg gcaagttcgc
     5401 ctatatggcg cccgagcagc ttcgtgcgaa gccggtcgac tcgaaagccg acgtctttgc
     5461 gatcggcgtg tgcctatacg aagcgatgac cggaaagcgt ccgttcggcg gcggaaccga
     5521 aggcgagctg atcgcgcgtc gcctcaccgg cgagttcgta acgccgtcgg aggtggtcca
     5581 caaattcccg gcggagctcg agcagatcat cctcgacgcg atggcgccgg atcccaccgc
     5641 gcgcccgtct gcgctcgagc tgcatcaacg tcttgcggcg ttcgcatcgg cggcgccgtt
     5701 tcagtcgagc tcgatcgcgg ttggcgcgtg gatgcgcgag atgttcgacg acgattcgag
     5761 cgcctacgac tcgatcgagt cgagcccatc gatgacgccg gtgccggcaa gcttgccgac
     5821 gggtgcgacg ccgcggccga gcgcctcgac ggtatcggtc gaccgggcga caccaccacc
     5881 ggcgcacacg cctcgacgtg gccgctggat cgtcggcgtg ctcgcagccg cgaccgcgct
     5941 cagtgccacc gcagcgggca tcaagctcgt gcggcacgac gatcacgctg catcgccagc
     6001 gcccgccccg atcgtcgcga ccgcaccgcc gcctgcgccg gcacccattg tcgcgccgca
     6061 gccggcacca ccgcctcccg cagcagtcgc gccgcccagc gaggtgcctg cagctgcggt
     6121 cgctgcgccg gctcccgacg cgcctcctcc tgaagaggtc gtcacggtcg agcgcaagcc
     6181 cggtccgacg aagatcgtgc gacgaccgac ggtcaaaccc aaggccacct ccacgaaggc
     6241 agcccctgtt gcccaaggct cggcgacaat ctccgaaacc aagcctgttg caccagcagg
     6301 ctcggcacca tccacgcccg cgatcaccgt gccaccgccc acgggttctg cgacgacgac
     6361 gatcacgatc accacgaccg cgccacagag cgtgacggtc aacaaaccgc caccgaccgg
     6421 gccgaccgcg acgaccgcgc ccgcgatcgc tgtgccgacg ccggcgagcc ctggttcgct
     6481 cgacgcagtg ccatcgatcg tgcgagtcag cgtcgacgga tcgctgacga cctccgaggt
     6541 acagacggcg ctcggtcgca cccttgatgc gctgcgcgcg tgttaccgca cagctgcaaa
     6601 gcaagcgaag agcacgccgg atctgaccgt gaaggtgctc ttcgagatcg acgaaggcgc
     6661 ccgtgcgtcg cgagtgcggc tgagcggcga cacgctcggg ctctcgagct gcgtttctgc
     6721 atccgtcggc agcctccgga cgcgcgttgc gcccgacgtc ggcaccgtct cggtgaccgc
     6781 gaccattcgc ttcaaaccca cccgctagct cgcaggaagg atctctgtga accgacgacg
     6841 attgatgtcc gcggtgctcg tgctcgctgc gcccgttgtg tggcgagtga gcgcggcaca
     6901 gccgcaaccc gatccgcttg cccccggagc cagcgttccg accgctcccg tgcccgacgc
     6961 cggcgtgcaa cccgatgcac ctacggaggt cccggtcacg ccggcgcccg caacgcctgc
     7021 gatcaccgcg ccgctcgagg ccgtcgcacc cgaaccggat cccgaaccgg agccggagcc
     7081 cgatcacaag ccgcagatgc atcgcaactt cgcgggctcg atccaactcg attacatgtc
     7141 cgtcgcgaca gagaagacgg gccgcgatgt cgcgctcgac ggcgcgacgg cagaagtgtc
     7201 gatgaagctc gcgatggact tcagcagcaa gatctctgcg aacgtgaaga tgtgcgtcgc
     7261 gtgccacggc gtcgaggtcg gcatggcgta tttcgacatt cgtcttgccg acgagatgaa
     7321 cgtgcgcgtc ggccggttca cgccgtcatt cggcgagttt cctgtccgcc acgatcccgc
     7381 gaaccaccgc acgagcgaca agcccctgcc ctacgacatg ggccgcatgc tgcgcatcac
     7441 cgagtggaac gaaggcgtgc tgcccgcacc atgggtcgac aacggcatcg agatcaacgg
     7501 cacgcacttc ttcggcgagc acgtacagac cgactacgcc gtgtatgcga tcggcggccc
     7561 gcgcgccggg tccgatccgg tcgacttcga tttcaagcgc tcgcgatccg gcgagagcta
     7621 ctacatcgac aacaactcgc gacccgcgat cggcggccag ctcagcgcga gcatcgcaac
     7681 ggacacgacg accacgtcga tcggcgcgtc gatgatgcgc ggcacgtacg atccagagca
     7741 ccgcctgcca ttcgcgatct acggcgcgca tgccgtgttc cgcgttcgcg acgtgttctt
     7801 gcgagccgag tatttatggc gacgcacgaa gatgtcactc ggcgacgatc cgatggagcg
     7861 gtttcgttac gcgccgggct cgaccggcat gttcgatccg tacttcgtca aggacggcgc
     7921 atacgcggag ctcgagatac cggccggacc acgcgtgacg ctcatatggc gcgaggacgg
     7981 gatgcggcgg cgcggcaacg tcgtctcgac gagcgacatg cgctccgaca gcgccatcgt
     8041 ccgtcacacc gcaggcatcg cgatcgcgat ccgctcggcg ctgcgtctca aggcgagcta
     8101 cgagtactac gacttttcag actacgagga cgagtcggtg ctgcacctcg gcatcgccgg
     8161 tccgttctag gaggccacat gaagtcgaca cgccttcttt acatcgtcac gctcgcgggt
     8221 tgcgacatgc tcggcccgcg cacgagcgac atcgcgatcg atgcaccgcc gagctcgcac
     8281 catgatgcag cggtcgacgc gtcgacgtat ctgctccccg ctggctccga cgtaccgcgg
     8341 atcacgacga acgccgagct cacgagccag atcaagatca acgatggcct cagcgatagc
     8401 gcgctcgcgg ctgcgggcgg tgtcatcacg cgcagcaccg gcaaggcgag cggcgcgacg
     8461 gtcatgtact ggaacttcgg cgccgcaccg ctcgcggaaa acttcgtggc atccgctccg
     8521 ctctacgtgc tgtgcgacga cgacggcaac ggcaacctca cgccgcgcac gggccatcca
     8581 tatctgatcg attcgattcc gggcgatccg cggtacagcc cgatccggcg gatcatcaac
     8641 gtgcccgtca cggcgtcgta caacggccag ctcatcacgt ccgtcgaagc gctcgccgaa
     8701 gcgtacgacc gcgggctcgt cggtgagccg aaacccggag gcacgtggcg cgacatgccg
     8761 gtggtaccga caggcacact gctcgaggtc agcagcaccg ttgcgccggc cgccgcgacg
     8821 gaggtgtacg ggcgcgggtt tgtcgtcgag ctgttcccgc tcggcggcgc gctcgggatc
     8881 cagccgctgc gcaacggcgt gatcccgatc ggccaagagt cgcgtctgct ctccggcgtc
     8941 gcgaccggca tgccgcccgt gatgccgacg gcgctcgatc cgcagccggt gttccagtac
     9001 gggatcccag ccgcgcctcc gacgacgacc ttcaactgga cgccgatcgt caccgagctc
     9061 gacgtgcgtc tcgcgagcgg cgtggatccg acaacggtga cgaatgacgc gagcctgttc
     9121 aagcggtcca cgacgggcgc gatcactggc tactacacga cgaccgtcga caactacacc
     9181 gtgatgacga ccgtctcgaa caaaccgatc caattcgtgg atggtgcacc atgaagtcga
     9241 gccttctcct cgtcgcggtc gccgcgtgtg acatcacgta cgtccccgac gtcggaccgt
     9301 tgcagaacga gccagctgcc gatgtcgacg gcggtaccgc tggtatgaat cagccgctcg
     9361 cgcgatgcat cgacagcgat ccgtccacgg acgtttcgtt ctcgctgcag attcgtccgc
     9421 tcacgacgcg ctcaccgggt ggctgcctga gctgtcacgg gacgaacacg acgtcggggt
     9481 tcaatctcgg ctcgtacgag tcgctgcgcc gcggcgggct caactccggc acgcgcatca
     9541 tcgtcgccgg tgatccatgc gcgagcattc tgccgcagaa gctcggcgtt gctcctccgt
     9601 acggagcgcg catgccctac aacggcccgc cgtacttcac gcccgcggag ctgacgctcg
     9661 tgcgtgactg gatcgcggaa ggagcagtcg acaattagga ccttcacatt cgttgtcttg
     9721 gttctcgtcg ctgggtgcgg agaaccgctg tctcgcacgt cgctcgtcgc gagccccgag
     9781 acgtcgcccg acgtgcggcg cgcgtgcgcc gtcaccgagc agaagtgcac gcggtgtcac
     9841 acgctcggac gcgtgctcgc cgcggacgcg acgacgcgag atcagtggga gccgatcgtg
     9901 ctgcgcatgc ggcgcatggc atcgagcggc atctcgagca acgacgcgga cgtcgtgctc
     9961 cactgtctcg cgtctcgtcg ctgagctact ggctgttcgg agcgttgccg ctcgtgccgg
    10021 acgttccgct cgtgccggac gttccgctcg tgccgctggt accggtgcta ttcgtaccgt
    10081 tcgtaccgtt cgtgctcgtg ccgctcgggc cggcagtacc gttgtcgttc gcgccgctgt
    10141 cgtccatgcc accggtcgtc ccggtgccct ggttcgagtt tgcgcccgtt ccagtgcctg
    10201 tgccggtgcc gctattcgtg ccaccggctg taccgcgcgc cgttcctcga cgagcagcac
    10261 ggttctgatc ggtgatcgtg ccgcttgtcc cgctcgtacc gctcgtatcg ctcgtaccgt
    10321 tgacgctgcc gccggcggtt acgccggtgc ccgttgtatc gacatcggtg ttcgcggcgg
    10381 tgccgctagt cccggtgccg ctgttcgaca tgccgctgtt cgcggtgccg ctcgccgtgt
    10441 cgttggcggt cccttcttgc gagccgggat tcgtcgcagt gtctgcgcgg ccggccgtgc
    10501 ccattcctcc ggtttcaccg cctacggtgg tgccgttcgg aacggtgtgt ccgggcgccg
    10561 tcgtcgtcgt cgtgtcctcg ttcgtccgtt gatacgtgct tgtctcatct gattgattgc
    10621 gatctcgctt gcagccgacg gcagcgccga cgacacagag cgagaagatc gctgaacgtg
    10681 taaatgtcat tgcattcctc ctgcacggag gcatcgcaac attcgtgcac gtcagagatt
    10741 cgaatttccc tcggcgaacg gagtggtgcg gatctgatcg gcccagcgcc cgcgatagcg
    10801 catgacgtac agtccgtttc gcacgtcgac gacgtagatc agcccatcct tgatgatcgg
    10861 atagctccac atctcgacat ggctgccgcc gaggccaggg tcctcgacag ccaccgctgc
    10921 gagcggctcg gggcggaatt ccgcgagctg cgtcggattc gcaggatcgg aaatatcgac
    10981 ggcttgaagc ccgcccgcgt accacgtgac gatcgccaga tcgtgcgtca ccgtcgcgtt
    11041 gtgcgaagtg aacgcgatca tcggcccgtc cgccgcgcac gctggatcgt tctcggcgag
    11101 cttgtattcg cctagcaaca tcggtcgcgt tggatctgca atgtcgacca tgcgcagatg
    11161 accccacgga catcccgcgc cgaacggcgg tggatagact tcttcggtcg tcacgaggag
    11221 cgagcgacca ggaaccggca ccgcggagtg cggccccatc gcgccgaact tcggcgcgtt
    11281 cgcggcgggc gtgagtgtcg tcagcgtcgg gaagctggtg agatcgacga cgaacagccc
    11341 cgcaacttga tgcgagagat acgcgctctt cccgtcatcg gagattgcga tcgaatgcag
    11401 aatgttgttc gcaccaccct tggggacgcc gccctgcgcg tacggatccc agcgcaagac
    11461 gttcgtcgcg gtcgccacat cgatcacctc gagtgacggc acgcccggcg ccgctagata
    11521 gatcagcgag cgtgatgcat cggcaggatc gcgccacaga aacatctcgt gcggcgagcg
    11581 cggaaacatc tgcgacgttc cctgcacggg gtacgacgct cggacgacgg gcgcgcgcgg
    11641 atcggtgatg tcgaagaagc gcaggttctc gacctcgccc gccgatgtcg cgcagccatg
    11701 gagcgacggc gagcactgca gattcatcac gacgagcgtg ttgccgacga cgcgcagctc
    11761 tcgcgacgac atgcccggca ggccttcgag tggcgtcccg atctcgccga ccacggtcgg
    11821 gtttgccggg tcggcgatgt cgacgatcaa gatcggcttg ccgtcgatgc gcgagccgac
    11881 atacgcggtg tctcctgcga tcgcgatcgc ggaattcatg ccgcgcccac cgaggtccgt
    11941 atgcccgacg agctcgaacg agccggtgcc cgcgaggttg tcgccgcacg ccgcgagcac
    12001 gagaagtagc gcagcgcgtc tcatgtaaac gaaatctcga cgggcatcgt cggcggctct
    12061 tgcgtgcggc gcagcgcgat ctgcagatcg ccacctgtcg cgaacagcgc gccgacgcct
    12121 cgcaccttcg agatctgctt tgccagctgt tgagcgggct cgggcgcgtc gagc
//
LOCUS       DATLBZ010000409        12304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1984682, whole genome shotgun sequence.
ACCESSION   DATLBZ010000409 DATLBZ010000000
VERSION     DATLBZ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12304
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984682"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VMZ53_20635"
     CDS             complement(<1..497)
                     /locus_tag="VMZ53_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30935.1"
                     /translation="MTALTTTDAFFVAYQQQRGILMHFGVEIELAAPPSRAAIDRAVA
                     QTLARWPTLAQTLAPRALGGLQWTGAAQPVIHTGTRDDVTRFRNAPIDPFREPPFGVL
                     WHRNTLAFRCHHAAADGELFIEIVRHFLAALSADVPTSTKPIDHPLALRHLRKQATVR
                     NSLRYA"
     gene            complement(497..1819)
                     /locus_tag="VMZ53_20640"
     CDS             complement(497..1819)
                     /locus_tag="VMZ53_20640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUS30936.1"
                     /translation="MKVDIIHPAHYRDDGSLVQAKSLIHRLDSYLPHLGPPLMAAHTP
                     AEHDVRIVEEYHRDVDFDTDADLIGISAQIMQFDRAVDLSREFRRRGKKTVLGGYLPS
                     LMPDRVQGLFDAIVIGEGDEVWPELIADAARGQLKPRYKPTRPVDLTKLPVPRYDLID
                     SRGRIVVYPVQATRGCPFTCTYCSIAAVYRGGYRKRPIADIVRDVEATGSRNINFCDD
                     NLCEDFKWAPQLFGALEGMKLRWGTQTTINVARHRNVLDAARRSGCMLMALGVETISA
                     RNLEEVDKTFHDVDKYADAFQRIMDAGITPHALIIFGLPEDNSETFKRTVDYLERLKV
                     PIAQFFIMTPYPGTPAGDKLWREDKVFDDRLAHLREPYVVYHPQQLTPTQLHDGWWSA
                     LESFYSLPSIARRVLFRKKPNNLLVNIGQNLLYWSKIRRGIHPVYFDN"
     gene            complement(1816..2814)
                     /locus_tag="VMZ53_20645"
     CDS             complement(1816..2814)
                     /locus_tag="VMZ53_20645"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HUS30937.1"
                     /translation="MKRLVLFVALFAVLAGAGIVWSVRRSGVRPGDLGQLWSLPVVSL
                     VALFVLCLGLYATDMVRYRLFARAIGEKVDWRGALDTSVANFFFSWITPGAALGAPAA
                     IVMLGRRGVTWEGATLIAFGKSVTGTVVLVALAFIAMACGYGPELDGRVFAVFMGGIA
                     AVSPLLLIPLIGVFWPARLLRGIERLERRFTGPIMTKVCGVLRRTVERLGKLRAGGAG
                     MPVMLLASHVVYLAVLVGIAVVLAVGFGATSVAAAIGISTVYAAFSYVAPTPGGAGLS
                     EAAATVFYGSILPAREAVLVVLLFRALTFYLGIAIGLVYLPVVGGTRQILAAKKTA"
     gene            2927..5815
                     /locus_tag="VMZ53_20650"
     CDS             2927..5815
                     /locus_tag="VMZ53_20650"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HUS30938.1"
                     /translation="MSTPSVHLAVLVVATDPAKADLGTAMVVRGRANGAGHQIVQQTV
                     TKDAEANIRTALARWMDDTNIDVILVIGAVESDSVPSAIKPLVTQRIEGFTDLFRFLA
                     FQEMGAGAMLSNAEAAQCGTTFVFVLPGGVGAVGTAMDKLIIPQLDKNTQPKNLVSQM
                     PRIAAAALGVEVEAVPAEVAPEKTAGGSGLVPKLPARANSATGRNVIRRSQDDPPTKQ
                     IDMAKLERQLERSKSPNDAQTKPAIDIRKMLPKLPPGADGNIDTDTDVELLAAPPPPL
                     MGTSRIDTSPPIPPPRPSPASLPSIKGNAFNSKEGTSPGTPPPSKTGSRPIPSISSSL
                     GSSPSASASLTGIKGSAFEAKEGTSPGAAAPTKPLGGPRSRPISVPPTNAFGGKEATS
                     PGVAATPPSSSSSSSPSSRKDDTTPGRPVPSRPPARPPTSPPATPASASKIAPIAATG
                     GALHTAVTPVAGVPRTAATPVGGVATSESAKTEMAAKSEPAKEFARPISSGMIGLPSS
                     QVFAAATKEAEPATPKTNGAAKPSTTTASTSPASTSKSTPSVEMAPLVEPDELHEDEL
                     EAAEDSHEAEESAEESAEEKAAWARARAASEPAADEAATVPRKREPTPPPPVAAHVIA
                     RAELDKEKDKAPPPTRRWEPTQKVDPMPITAAMRDSSGELPLGTFNYPVQKSRGGASA
                     LKWLLLLFALGAGFGAVVFVAPKLMKSGDKKPAAQVAQTTPPAPQHVQTGSAGAPVGT
                     PPDESATPDAAVGDTPPVETASPQTPTTTTTTTTSTTSTTNRGGTTNRGGTPNRGGTP
                     NRGGTTTASGPVTNPTTNTTTNTNTTPPDDKPIETKTPPPPSDPTCDEVSCVLSKYDR
                     PCCERYKPKDSDLGKRSGSIPEDLDRASVRAGMEKVKPAIVQCGEKAGVQGTVKITVS
                     VAPEGNVTSSEVAESPDPALGSCVAAAIKRTSFSKSVNGGSFTYPFVF"
     gene            complement(5823..6521)
                     /locus_tag="VMZ53_20655"
     CDS             complement(5823..6521)
                     /locus_tag="VMZ53_20655"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HUS30939.1"
                     /translation="MIRIRRANERGRANHGWLDSHHTFSFADYYDPEHMGFRSLRVIN
                     DDRVKGGNGFGSHPHRDMEIISYVLEGALAHKDSTGTGAVIKPGDVQRMSAGTGVVHS
                     EHNASKTETVHFLQIWLLPEKPGIAPGYEQKTFSDADKRGTLKLVAARDARDGAVKIH
                     TDASVYAGVFDNGERAELPIAAGRSAWVHVARGEVNVNGNVVKDGDGVALTDEANVVV
                     EGTTGGEVLVFDLA"
     gene            6642..7319
                     /locus_tag="VMZ53_20660"
     CDS             6642..7319
                     /locus_tag="VMZ53_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS30940.1"
                     /translation="MVESTPESFRGEQGADFAKHQLQGRPRILHVGRPACSVPGRLAD
                     VGFAVTATAGEELATLDDSFDAIVFSHALSTAESLDTTIEHAARLLSATGRLVVDDID
                     AQAIDMMSARWFYDLQELLAVTGLYPSDRVHRPNADPIARWRDGTRADGIAHSGTEMR
                     IAISARFAIRDLKRVEGFYRHISDGLPADSRGAAVASHVRGVERRLIASDALLPVGLR
                     VVADRTR"
     gene            complement(7316..7678)
                     /locus_tag="VMZ53_20665"
     CDS             complement(7316..7678)
                     /locus_tag="VMZ53_20665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS30941.1"
                     /translation="MRVLVVEDHAALAEAFAESIRRNGHEAFVAITGASAVTMARTHA
                     PHAVLLDIGLPDMDGYVVARTMREQGLSPSTVIIMVTGKTLEPGGDAGVDLVLQKPVE
                     SEMLAGLVEYLSRRTTRP"
     gene            8074..8745
                     /locus_tag="VMZ53_20670"
     CDS             8074..8745
                     /locus_tag="VMZ53_20670"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUS30942.1"
                     /translation="MFRAGERLAEALGHKQPVTPHTLRVGLSGAVGRSTTTDFLMPLL
                     ELEDCVPSIRAGDTMELMRELRSGELDLVLCESEPPEAARRGLEVVLLERITLVAVAP
                     PDLNPAPEWQDVNLVHYRSSSAFRWEVETFLETRSLRPRVVAEADDALFLVEAAARGR
                     HVAFVPRSVARDAIATGRLKQVAQLEAGHAGVHALYQDGATAELARRAVEVLIEHLRS
                     SQLDA"
     gene            8766..9644
                     /locus_tag="VMZ53_20675"
     CDS             8766..9644
                     /locus_tag="VMZ53_20675"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUS30943.1"
                     /translation="MLNYNHLHYFHVAATEGSVAAAAERLGVTQPTVSEQVRALERAL
                     KVSLFERSATGLKLTEAGKIAYEQTSVMFRAGERLVESLGHGPSPVPRSLRIGISNAV
                     TRTTTTDFLLPLLALEDCLPSIKTGEANELLRDMRGNELDVVLCESEPPEASRRGLEL
                     IQIARTTLVAVAPPTLEPDTEWRNAGLIHYRASSSFRWDVEAYLELSDLRPRIVAEAD
                     DPLFLVEAAARGGYIAVVPRSVARDAISQGRLKQLATVESTQAGVYALYQDGATAELA
                     RLAVQLLMDSAKNALT"
     gene            complement(9641..9994)
                     /locus_tag="VMZ53_20680"
     CDS             complement(9641..9994)
                     /locus_tag="VMZ53_20680"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUS30944.1"
                     /translation="MRFVVLCYDNQEEILAWSAEKDAQVMQQLGAVNDRLDAAGKLGP
                     VVRLADTAKAKTIRKGKQAVITDGPFAETKEQLLGFYIVEAETIDEALSIGKDLAAAS
                     GSAGAFEIRPLIYYR"
     gene            10038..10913
                     /locus_tag="VMZ53_20685"
     CDS             10038..10913
                     /locus_tag="VMZ53_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30945.1"
                     /translation="MRIAAALLLVVSGCDILFQVQTVHLQPDATIDAHVVIDAPGCWD
                     AGYKGNEDNDTKIDGCDNCPLDDNEDQLDTDGDGVGNVCDPHPSEPLEQRALFHPMTR
                     YDAAEWVVRGNGNWQTNTSINDLGIKQANVAVKATLLLKGKKFDQPYVELLFRQGNLE
                     QATSTVGAYVTRDGDTSTLAISDGVACGLVGIEPRAYGHVKTNDMIFSVDAPLGGMTP
                     SHMGVGTRVAGKAPADPVLPTCHVDRVGGGNKVEANPVLPLEISGADRMNVGVYVIDA
                     SVTFTGMVVLERRAP"
     gene            10910..11680
                     /locus_tag="VMZ53_20690"
     CDS             10910..11680
                     /locus_tag="VMZ53_20690"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS30946.1"
                     /translation="MNEDFSDERRQSFDKAAAAYDKIRPGYPEAAIDDILERSQAVRA
                     LEVGAGTGKATLAFARCGVEVHALEPGGKLADILRAKTIGLPVTIEETTFEAFEGGEF
                     DLVFSAQAWHWTDPATRYQKAHAVLQPGGALALLMNEKAPVEQGLRDDFDAAYAKWFG
                     WPAWDNDHLPKLVDKWSDEIKASKLFRDLVVTYVPWTQSYTSDEFIELLATYSDHAVR
                     PEEQRRGCYADIKAAIDKRGGRCEIPYVTLAFFALRCD"
     gene            11680..12288
                     /locus_tag="VMZ53_20695"
     CDS             11680..12288
                     /locus_tag="VMZ53_20695"
                     /inference="COORDINATES: protein motif:HMM:NF025611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HUS30947.1"
                     /translation="MRLLVYDKTCKGGNGGNLTYAWSAGSKLYKGLQRVDAAHGVTSW
                     DEALDWIASHKEPIDEIQYWGHGKWGYALVDDDRLDASSLVGPRRAKLEAVRERLTPN
                     ALLWFRCCEVFGANRGIDFAERLTDFLGVRVAGHTYIIGFHQSGLHGLSPGVRADWSP
                     EEGIVEGTADAPVQGKWSKPWAPRTISCLSNTVPAGWFTMPA"
BASE COUNT         2253 a         3936 c         4130 g         1985 t
ORIGIN      
        1 gcgtagcgga ggctgttgcg aacggttgct tgcttgcgga gatggcggag cgcgagcggg
       61 tggtcgatcg gttttgtcga cgtcgggacg tcggcggaga gagccgcgag gaaatgccgc
      121 acgatctcga tgaagagctc gccgtcggcc gcggcgtggt ggcagcggaa cgcgagcgtg
      181 ttgcgatgcc agaggacgcc gaatggtggt tcgcggaatg gatcgatcgg cgcgttgcgg
      241 aagcgcgtga cgtcgtcgcg cgtgcctgta tggatcaccg gctgtgcggc gccggtccac
      301 tgcaagccgc cgagcgcgcg aggggcgagc gtctgcgcga gcgttggcca gcgcgctagc
      361 gtttgcgcga cagcgcgatc gatcgcagcg cgagagggcg gcgcggccag ctcgatctcg
      421 acgccgaagt gcatgaggat gccgcgctgc tgctggtacg cgacgaagaa tgcatcggtg
      481 gtcgtgagcg cagtcatcag ttgtcgaagt acacggggtg gataccgcgc cggatcttcg
      541 accagtagag gaggttctgg ccgatgttga cgagcaggtt gtttggcttc ttgcgaaaca
      601 gcacgcggcg cgcgatcgac ggcagcgagt agaaggactc gagcgcgctc caccaaccat
      661 cgtgcagctg cgtcggcgtg agctgctgcg ggtggtagac gacgtagggc tcgcggaggt
      721 gcgcgaggcg atcgtcgaag accttgtctt cgcgccagag cttgtcgccc gccggcgtgc
      781 ccgggtacgg cgtcatgatg aagaactgcg cgatcgggac cttcaggcgt tcgaggtaat
      841 cgaccgtgcg cttgaacgtt tcggagttgt cctccggcag gccgaagatg atcagcgcgt
      901 gcggcgtgat gcccgcgtcc atgatgcgct gaaaggcgtc ggcgtatttg tcgacgtcgt
      961 ggaacgtctt gtcgacttct tcgagattgc gcgcgctgat cgtctcgacg ccgagggcca
     1021 tcagcatgca accggagcgg cgagccgcat cgaggacgtt gcggtggcgc gcgacgttga
     1081 tcgtggtttg cgtgccccag cgcagcttca taccttcgag cgcgccgaac agttggggcg
     1141 cccatttgaa gtcttcacag aggttgtcgt cgcagaagtt gatgttgcgc gagccggtgg
     1201 cttcgacgtc gcgcacgatg tcggcgatcg gtcgcttgcg gtagccgccg cgatagaccg
     1261 cagcgatcga gcaatacgtg cacgtgaatg gacagccgcg ggtcgcttgg actggataga
     1321 cgacgatgcg accgcgcgag tcgatcagat cgtagcgagg cacggggagc ttcgtgagat
     1381 cgacggggcg agttggcttg tagcgaggct tgagctgacc gcgggccgcg tcggcgatga
     1441 gctcgggcca cacttcgtcg ccttcgccga tcacgatcgc gtcgaagagg ccctgcacgc
     1501 ggtcgggcat cagcgagggc aggtagccgc cgagcacggt cttcttgccg cggcggcgga
     1561 actcgcgcga taggtcgacc gcgcgatcga actgcatgat ctgcgcggaa atgccgatca
     1621 gatcggcgtc ggtgtcgaag tcgacgtcgc ggtgatactc ctcgacgatc cggacgtcgt
     1681 gctcggccgg cgtgtgcgcc gccatgagcg gcgggccgag gtgtggcagg taactgtcga
     1741 gtcgatggat gagcgacttc gcttggacga gcgagccgtc gtcgcggtag tgcgccggat
     1801 gaatgatgtc gaccttcacg ctgtcttctt cgccgccaag atctggcgtg tgccaccgac
     1861 gaccggcagg tacacgagcc cgatcgcgat ccccagatag aacgtcaggg cccgaaacag
     1921 caagaccacg agcacggctt cgcgcgccgg aagaatactg ccgtaaaaca ccgtggccgc
     1981 ggcctccgaa agaccggccc cgccgggggt gggggcgaca tacgaaaacg cggcgtagac
     2041 cgtcgagatt ccaatcgcgg cggccaccga cgtcgcaccg aagccgacgg ccagcacgac
     2101 ggcaattccg acgaggacgg cgagatacac gacgtggctc gcgagcagca tcaccggcat
     2161 gccagcgcca ccggcgcgga gcttgccgag gcgttcgacg gttcggcgga gcacgccgca
     2221 caccttcgtc atgatcgggc cggtgaagcg ccgctcgagc cgctcgatgc cccgcaacag
     2281 ccgcgctggc cagaacacgc cgataagagg gatgagtaac agcgggctga cggccgcgat
     2341 gccacccatg aagaccgcga acacgcggcc atcgagctcc ggcccgtagc cgcaggccat
     2401 cgcgatgaac gcgagcgcga ccaggacaac cgtccccgtg accgacttgc cgaacgcgat
     2461 gagcgtcgcg ccttcccatg tcacgccacg gcggcccagc atcacgatcg cggcaggtgc
     2521 cccgagcgcg gcgccgggcg tgatccacga gaagaagaag ttcgccacgc tcgtatcgag
     2581 cgcgcctcgc caatcgacct tctcgccgat cgcgcgcgcg aacagccgat agcggaccat
     2641 gtcggtcgca tagaggccga ggcaaagcac gaatagcgca acgagtgaca caaccggcag
     2701 ggaccacagc tggccgagat cgccagggcg aaccccgctc cgccgcacgc tccagacaat
     2761 ccccgcgccg gcgaggaccg cgaacagcgc cacgaacaaa accaaccgtt tcacccgtgc
     2821 gggagcatac gtgtcgcggg gatggcagag gtaggtgaag tccgcgatct tgcgggcatc
     2881 ggacgcgtgc tcgacgacga gacgcgtgcg aatatcgagg ctgctcgtgt cgacgccgtc
     2941 cgtccacctc gcagtcctcg tcgttgcgac cgatccggcc aaggcggatt tgggcacggc
     3001 gatggtcgtg cgcggccgcg cgaatggcgc aggacatcag atcgtccagc aaacggtcac
     3061 gaaagacgcc gaagcgaaca tccgcaccgc gctcgcacgt tggatggacg acacgaacat
     3121 cgacgtgatc ctcgtgatcg gcgccgtcga gagcgactcg gtgccgtcgg cgatcaagcc
     3181 gctcgtcaca cagaggatcg aaggcttcac ggacctcttc cgcttcctcg cgttccagga
     3241 aatgggcgcc ggcgcgatgc tcagcaacgc cgaggccgcg cagtgcggca cgacgttcgt
     3301 gttcgtgctg ccgggaggcg tgggcgctgt cggcacggcg atggacaagc tgatcatccc
     3361 gcagctcgac aagaacacgc agccgaagaa cctcgtctcg cagatgccgc gcatcgctgc
     3421 agccgctctc ggcgtcgaag tcgaagcggt ccccgcggaa gtcgcgcccg agaagacggc
     3481 cggcggctcg ggcctcgtgc cgaagctgcc cgcgcgcgcc aattcagcga cgggccgcaa
     3541 cgtgatccgc cgctcgcaag acgatccgcc gacgaagcag atcgacatgg cgaagctcga
     3601 gcgtcagctc gaacgcagca agtcgccgaa cgacgcgcag acgaaacccg cgatcgatat
     3661 tcgcaagatg ttgccgaagc tgccgccggg cgccgacggc aacatcgaca ccgacaccga
     3721 cgtcgagctg ctcgccgcgc caccgccgcc cctcatgggc acgtcacgca tcgacacatc
     3781 gccgccgatt ccaccgccgc gtccatcgcc cgcgtcgctg ccgtcgatca aaggcaacgc
     3841 gttcaattcg aaggaaggca cctcgccggg gacgccgcct ccgtcgaaga cgggctcgcg
     3901 accgattcca tccatcagct cgtcgctcgg ctcatcgccg tcggcgagcg cgtcgctcac
     3961 cggcatcaag ggcagcgcct tcgaagcgaa ggaaggcaca tcgcccggcg ccgctgcgcc
     4021 gacgaagccg ctcggtgggc cacgctctcg tccgatttcc gtaccgccga cgaacgcgtt
     4081 cggcggcaaa gaggccacat cgccgggtgt cgccgcaacg ccgccgtcgt cgtcatcgtc
     4141 gtcatcgccg tcatcgcgta aagacgacac gacgccaggc cggcccgtgc cctcgcgtcc
     4201 acctgcgcgc ccgccgacga gcccgccggc aacaccagca agcgcgtcaa agatcgcgcc
     4261 cattgccgca accggtggtg cgttgcacac cgcagtcacg cccgtcgcgg gtgtgccgcg
     4321 caccgccgcg acgcccgttg gcggtgttgc aacctcggag tcggcgaaga ccgagatggc
     4381 tgcgaagtcc gagccggcga aagaattcgc gcgaccgatc agctccggca tgattggcct
     4441 gccgagctcg caggtgttcg cggccgcgac caaggaagcc gagccggcga cgccgaagac
     4501 gaacggcgcg gcgaagccga gcacaacgac ggcgagcaca tcgccagcga gcacgagcaa
     4561 atccacgccg tccgtcgaga tggcgccgct cgtcgaaccc gacgagctgc acgaagacga
     4621 gctcgaagcg gcggaagact cgcacgaggc ggaggagtca gcggaggagt cagcggagga
     4681 gaaggccgcg tgggcaaggg cgcgcgccgc atccgagccg gcggccgacg aagccgcgac
     4741 ggtgccgcgc aagcgcgagc cgacaccgcc accgccggtc gcggcgcatg tgatcgcgcg
     4801 cgcggagctc gacaaagaga aagacaaggc gccgcctccg actcgccgtt gggagccgac
     4861 gcaaaaagtc gacccgatgc cgatcaccgc agcgatgcgg gacagcagcg gcgagctacc
     4921 gctcggcacc ttcaactatc ccgtgcagaa gagtcgcggc ggggcgagcg cgctgaagtg
     4981 gttgctcctg ttgttcgcgc tcggtgcagg atttggcgcc gtcgtgttcg tagccccgaa
     5041 gctcatgaag agcggtgaca agaagccggc tgcgcaagtc gcacaaacga caccgcctgc
     5101 cccgcagcat gtccagactg gaagcgccgg ggctcccgtc ggaacgccac ccgacgagag
     5161 cgcaacgcct gacgccgcgg tcggcgacac gccgcccgtc gaaacggcga gcccacagac
     5221 tccgacgacg acgaccacga ccacaacaag cacgacgagc acgacgaatc gtggtggcac
     5281 gacgaaccgt ggcggcacgc cgaatcgcgg cggcacgccg aatcgcggcg gcacgacgac
     5341 ggcgtcgggt cccgtcacaa atccgacgac gaacacgacg acgaacacga acacgacgcc
     5401 gcccgacgac aagccgatcg agacgaagac gccgcctccg ccatcggatc ccacgtgcga
     5461 cgaagtctcg tgcgtgctct cgaagtacga ccgtccgtgc tgcgagcgct acaagccgaa
     5521 ggacagcgac ctcgggaaac gctccggcag cattccagaa gatctcgatc gcgcgtcggt
     5581 acgtgctggc atggagaagg tgaagccggc gatcgtccag tgcggtgaga aggccggcgt
     5641 gcagggcacc gtgaagatca cggtctcggt cgcgccagaa ggcaacgtca cgtcgtcgga
     5701 agtcgccgag tcgccggatc ctgccctcgg ctcgtgcgtg gcggcggcga tcaagcgcac
     5761 gagcttcagc aagagcgtga acggcggctc gttcacgtac ccgttcgtct tctagacgag
     5821 atctaggcga gatcgaagac gaggacttcg ccgcccgtcg tgccttcgac gacgacgttg
     5881 gcctcgtcgg tgagcgcgac gccgtcgcca tctttgacga cgttgccgtt cacgttgacc
     5941 tcgccgcgcg cgacgtgcac ccacgcgctg cgaccggcgg cgatcggcag ctcggcgcgc
     6001 tcgccgttgt cgaacacgcc ggcgtacacc gatgcgtcgg tgtggatctt caccgcgccg
     6061 tcgcgagcgt cgcgcgctgc tacgagcttg agcgtcccgc gcttgtcggc gtcgctgaac
     6121 gtcttctgct cgtagccagg tgcgatgccg ggcttctcgg gcagcagcca gatctggagg
     6181 aagtgcaccg tctccgtctt cgatgcgttg tgctcgctgt gcacgacgcc ggtgcctgcg
     6241 ctcattcgct gcacgtcgcc cggcttgatc acagcgccgg tgcccgtcga gtctttgtgc
     6301 gcgagggcgc cctcgagcac gtacgagatg atttccatgt cgcggtgagg gtgtgagcca
     6361 aaaccattgc cgcctttgac gcggtcgtcg ttgatcacgc gcagcgagcg gaagcccatg
     6421 tgctcggggt cgtagtaatc ggcgaacgaa aacgtgtggt ggctgtcgag ccagccgtgg
     6481 ttcgcgcggc ctctctcgtt cgctcggcgg atgcggatca tggttctgcc tttcaactcg
     6541 cttcggcttc tccgaaggat gagcaaagtg ttgttaacga aggtctacta ccccgtcgcg
     6601 accttgcgag tccggaatcg ttcggccgaa gatgcggacg gatggtggag tctacgccgg
     6661 agagcttccg gggcgagcag ggtgccgact tcgcgaagca tcagctgcaa ggccgtcctc
     6721 gcattctcca cgtcgggcgt cccgcgtgct cggttcctgg tcggctcgca gacgtcgggt
     6781 ttgccgtcac cgcgacggca ggtgaggagc tcgcgacgct cgacgattcg ttcgacgcga
     6841 tcgtgttctc gcacgcgctc tcgacggccg aatcgctcga cacgacgatc gagcacgctg
     6901 cgcgcctgct ctcggccacc ggtaggcttg tcgttgacga tatcgacgcg caggcgatcg
     6961 acatgatgtc cgcgcgttgg ttctacgacc tgcaagagct gctcgctgtc accgggctct
     7021 acccgagcga tcgcgtgcat cgaccaaacg ccgacccgat cgcgcgttgg cgcgatggca
     7081 cgcgcgccga tggcatcgcg cacagcggca ccgaaatgcg gatcgcgatc tctgcgcggt
     7141 ttgcgattcg cgatctgaag cgcgtcgaag gtttttatcg ccacatcagc gatggcctgc
     7201 ctgccgactc acgcggcgca gcagtcgcgt cgcacgtccg cggtgtggag cgacgactga
     7261 tcgcgagcga cgcgctgctg ccggttggcc ttcgcgtggt cgctgatcgc acgcgttagg
     7321 ggcgcgtggt gcgacggctc aggtactcga cgagacccgc gagcatttcg ctctcgacgg
     7381 gcttctgcag cacgaggtcg acacctgcat cgcctcccgg ctcgagggtc ttgccggtca
     7441 ccatgatgat cacggtggac ggcgagaggc cttgctcgcg catcgtgcgc gccacgacgt
     7501 agccgtccat atcgggtaag ccgatgtcga gcagcaccgc atgtggtgcg tgcgtgcgag
     7561 ccatcgtcac tgcgctcgcg ccggtgatgg cgacgaacgc ttcgtgtccg ttgcgtcgaa
     7621 tcgactctgc aaacgcttcc gcgagagcag catgatcctc gacgacgagg acgcgcatcg
     7681 gttcacgatt catcgaccgc agaattcctg ttgcgagacc ggactgcaag atcgccgtcg
     7741 gttttctcgt cctgttcggg aataccgaag gaggccataa ggtcatgatc ttttttggat
     7801 cttgagcgca acgcagcggt gcggcgatcg aacaaatggt gttagagcgt ttgtggttcg
     7861 atgctcaatt acaaccacct ctactacttc cacgtcgccg caacggcggg ctctgtcgcc
     7921 aatgccgcgc agcgactcgg ggtgacacag ccgaccgtaa gcgagcaggt gaaggcgttg
     7981 gagcgggtgc tcggtatctc attgtttgac cgccaacctt cggggctcaa actaactgaa
     8041 gcgggtcgga tcaccttcga gcacacctcc gtgatgttcc gagccggcga acgtctcgca
     8101 gaagcgctcg gccacaagca gcccgtgacg ccgcacacgt tgcgcgtggg tcttagcggc
     8161 gcagtaggtc gcagcacgac gaccgacttc ttgatgccgc tgctcgagct cgaagactgt
     8221 gtcccgagca ttcgcgccgg agacacgatg gagctcatgc gcgagctgcg cagcggcgag
     8281 ctcgacttgg tgctgtgtga gagcgagcca cccgaagcag cgcgccgtgg tctcgaagtc
     8341 gtgctgctcg agcgcatcac gctcgtggca gttgcgccgc ccgacctgaa tccggccccc
     8401 gagtggcagg acgtcaatct agtccattat cggagcagct ctgcgtttcg gtgggaagtc
     8461 gagacgttcc tcgagacgcg cagcttgcgt ccacgcgtgg tcgcagaagc cgatgacgcg
     8521 ttgttcctcg tcgaggcagc cgcacgtggc cggcacgtcg cgttcgtgcc acggtcggtg
     8581 gcccgtgatg cgatcgccac aggtaggctc aagcaagttg cgcaactcga ggcggggcac
     8641 gccggggtcc acgcgctata ccaagatgga gcgaccgctg agctcgcgcg tcgggcggtt
     8701 gaggtcttga tcgagcatct gcgatcgtct caactcgacg cttaggaaac tgatcggagc
     8761 gacccatgct caattacaac catctgcatt acttccatgt ggcggcgacc gagggctcgg
     8821 tggcagcggc tgcagaacgg ttgggcgtaa cccagccgac ggtcagcgag caggtgcgcg
     8881 cgctcgaacg agcgctgaag gtgagcctgt tcgaacgctc tgcgacaggc ctgaagctca
     8941 ccgaagcggg aaagatcgcg tacgagcaga cgtccgtgat gtttcgcgcc ggcgaacggc
     9001 tcgtggagtc gctcggccac ggccctagtc ccgtgccgcg ctcgctgcgc atcggtatca
     9061 gcaacgctgt cacccgcacg accacgacag acttcttgct ccccctgctc gcgctcgaag
     9121 actgcttgcc gtcgatcaag acgggcgaag ccaacgagct gctgcgcgac atgcgcggca
     9181 acgagctcga cgtcgtgctc tgcgagagcg agccccccga agcatcgcgc cgcggtctcg
     9241 agctcattca gatcgcacgc accacgcttg tcgccgtcgc gccgccgacg ctcgagcccg
     9301 acaccgagtg gcgcaacgcc gggctcattc actaccgcgc gagctcgtcg ttccggtggg
     9361 acgtcgaagc gtacctcgag ctgagcgacc tgcggccgcg catcgtcgcg gaagcggatg
     9421 atccgctgtt cctcgtcgaa gccgctgcac gcggcggcta catcgccgtg gtgccgcggt
     9481 ccgttgcacg cgacgcgatc tcgcagggca ggctcaagca gctcgcgacg gtcgagtcga
     9541 cacaagccgg cgtctacgcg ctctatcaag atggcgcgac ggcagagctt gcacgcttgg
     9601 cggtgcagct gctgatggac tccgcgaaga acgcgctgac ctagcggtag tagatcagcg
     9661 gtcggatctc gaacgcgccg gcagagccgc tcgccgctgc gaggtctttg ccgatcgaga
     9721 gcgcttcgtc gatcgtctcc gcttcaacga tgtagaagcc gaggagctgc tcctttgtct
     9781 cggcaaacgg gccgtcggta atgaccgcct gcttgccctt acgaatcgtc ttggctttcg
     9841 cggtatcggc gagacgcacg acaggaccga gcttgcccgc agcgtcgagc cgatcgttga
     9901 ccgcgccgag ttgctgcatg acctgcgcgt ccttctcggc ggaccacgcg aggatctctt
     9961 cctgattgtc gtaacagagg actacgaacc gcatcggtcc gtactaccac gactcgcaaa
    10021 atgagatgat cctggcgatg aggatcgcag ctgctctact gctcgtcgtg agcggttgcg
    10081 acatcctgtt ccaggtgcag accgttcatc tgcagccaga cgcgaccatc gatgcgcatg
    10141 tcgtgatcga cgcgccggga tgctgggacg ccggctacaa gggcaacgag gacaacgaca
    10201 cgaagatcga cggttgcgac aactgcccgc tcgacgacaa cgaagaccag ctcgacaccg
    10261 acggcgacgg cgtcggcaac gtctgcgatc cacatccgag cgagccgctc gagcagcgcg
    10321 cactatttca cccgatgacc cgctatgacg cggcggagtg ggtcgtgcgc ggcaacggca
    10381 actggcagac aaacacatcg atcaacgacc tcggcatcaa gcaggcaaac gtggccgtga
    10441 aagccacgct gctgctcaag ggcaagaagt tcgatcagcc gtacgtcgag ctcctgtttc
    10501 gccaaggcaa tctcgaacaa gccacctcga cggtcggcgc atacgtcacg cgcgacggcg
    10561 atacgtcgac gcttgcgatc tccgatggtg ttgcgtgtgg gctcgtcggc atcgagccgc
    10621 gcgcgtacgg ccatgtgaag accaatgaca tgatcttttc cgtcgacgcg ccgcttggcg
    10681 gcatgacgcc ttctcacatg ggagtaggca cgcgagttgc gggcaaggcg cctgccgatc
    10741 cggtcctgcc gacgtgtcac gtcgatcgcg tcggcggcgg caacaaggtc gaagcaaatc
    10801 ctgttctgcc gctcgagatc agcggtgcgg atcgaatgaa cgtcggcgtc tatgtgatcg
    10861 acgcgagcgt gacgttcacc ggcatggtcg tcctcgagag gcgagcgccg tgaacgaaga
    10921 cttcagcgac gagcggcggc aatcgttcga taaagcggcc gcggcgtacg acaagatccg
    10981 tccggggtat ccggaggccg cgatcgacga catcctcgaa cgcagccaag cggtgcgcgc
    11041 gctcgaagtc ggcgcaggca cgggtaaggc gacgttggca ttcgcgcgct gcggcgtcga
    11101 agtccacgcg ctcgagcccg gcggcaagct cgccgatatc ctgcgcgcaa agacgatcgg
    11161 cctgcccgtg acgatcgagg agacgacgtt cgaagcgttc gagggcggcg agttcgatct
    11221 cgtgttctcg gcgcaggcgt ggcactggac cgatccggcg acgcgctacc agaaggcgca
    11281 tgcagtgctc cagcccggcg gcgcgctcgc gttgctgatg aacgagaagg cgccggtgga
    11341 gcagggcctt cgcgacgact tcgacgcggc gtatgcgaag tggttcggct ggcccgcgtg
    11401 ggacaacgac cacctgccga agctcgtcga caagtggagc gacgagatca aggcgagcaa
    11461 gctgtttcgc gacctcgtcg tcacgtacgt gccgtggacg cagtcgtaca cgagcgacga
    11521 attcatcgag ctcctcgcta cgtacagcga tcatgcggtg cgtcccgaag agcagaggcg
    11581 cggctgctat gccgacatca aggcggcgat cgacaagcgc ggcggccgct gcgagattcc
    11641 ctacgtgacg ctggcgttct tcgcgctacg ctgcgactga tgcggctgct cgtctacgac
    11701 aagacttgca agggcggaaa cggcgggaac ctgacgtatg cgtggtcggc cggcagcaag
    11761 ctctacaagg ggcttcagcg cgtcgatgct gcacacggcg tgacgagctg ggatgaagcg
    11821 ctcgactgga tcgcgtcgca caaagagccg atcgacgaga tccaatattg gggccacggc
    11881 aagtggggct atgcacttgt cgatgacgac cggctcgatg cgtcgtcgtt ggtggggccg
    11941 cgtcgcgcaa agctcgaagc tgtgcgtgag cggctcacgc cgaatgcgct gctgtggttt
    12001 cgctgctgcg aggtgttcgg cgcgaaccgc ggcatcgact tcgccgagcg cctcaccgac
    12061 tttctcggcg tgcgtgtcgc aggtcacacg tacatcatcg gttttcacca gagcggcctg
    12121 cacgggctgt cgcccggtgt gcgcgccgat tggtcgcccg aggagggaat cgtcgaggga
    12181 acggccgatg ccccggtgca gggcaagtgg tcgaagccat gggcgccgcg cacgatctcg
    12241 tgtctatcga atacggtgcc ggctggttgg tttacgatgc cagcatgagg gttctcgctg
    12301 tcgt
//
LOCUS       DATLBZ010000410         3146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7699432, whole genome shotgun sequence.
ACCESSION   DATLBZ010000410 DATLBZ010000000
VERSION     DATLBZ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7699432"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VMZ53_20700"
     CDS             <1..642
                     /locus_tag="VMZ53_20700"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30948.1"
                     /translation="GPVQLSTLAHFRSGPVKNRYDLSRALADVFRAEIGEVVQAGCKH
                     VQLEDLGAWMPHLSGEKDWDWVSETVDRTMAEIPQGVERSWHFCMGNAWGNKLEGMTE
                     GGYREVLPHYHGVKVDAYVLDFAAREMEDADLLRKLPADKKVHVGVIDVRMLEIEHPE
                     QVAERVRKVLKHIEPERVTLTTDCGLKQLPRVVAVQKLRALVEGAAIVRRELR"
     gene            complement(1221..1427)
                     /locus_tag="VMZ53_20705"
                     /pseudo
     CDS             complement(1221..1427)
                     /locus_tag="VMZ53_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(1437..>3146)
                     /locus_tag="VMZ53_20710"
     CDS             complement(1437..>3146)
                     /locus_tag="VMZ53_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30949.1"
                     /translation="MLLVIPPFAFCALSGMEVAFAAALLVASLLLLVDQSADGPPTKR
                     LVACLAAASLSRPEAMLLVGAIVGIAVLARLRKRAWKPAAWWLLPLVPPIVWLLANRL
                     IAGNFFPNTGVAKSHFYLPGFDWVYWKLTVVDLTKKMFRGLFWEKTSPLLWPRVVLAL
                     FLFGSARIAWWAWREKKFLVGALVILAPFMMMMSVVASSGLWSFQNYRYIAPALPLLF
                     IPVGFAFAPPDSLPAALTPYRRWIRRGWVAASSVLVVFFILAARKPLVADMRLFAQGA
                     MDTNTQVVAIGNYLHRKLPDARVMFHDAGAIAYYGDGEVYDMLGLVTNHQAGIANNGP
                     GARFEFLESIPPDRRPTHFAYYPGWMGTPEFYGEALMHTSLRRGIDPSGRRLVGEGDM
                     QILTAVWDHAGTAERPLNDHTGWALVDRLDVADIASERAHDWVGKMGRRKFGDPTARW
                     SVVEKETAHGLLIDGGRTIRDGGETFTVHVVPDKPTRIVIRTGGQPTYPWHEQITKPV
                     QMTLWSGTKKLGELTVAPPTGQFSELTFNVQAHALPQAASEIRVEASGIYRVFHWFVL
                     QPE"
BASE COUNT          582 a         1032 c         1037 g          495 t
ORIGIN      
        1 ggtcccgtcc agctctcgac gctcgcgcac tttcgctccg ggccggtgaa gaatcgctac
       61 gatctctctc gcgcgctcgc cgacgtgttc cgcgctgaaa tcggcgaggt cgtgcaggca
      121 ggctgcaagc acgtccagct cgaggacctc ggcgcgtgga tgccgcacct ctcgggcgag
      181 aaagactggg attgggtcag cgagaccgtc gatcgcacga tggccgagat tccgcagggc
      241 gtcgaacgct cgtggcactt ctgcatgggc aacgcgtggg gcaacaagct cgagggcatg
      301 accgaaggcg gctaccgcga agtgctgccg cactatcacg gcgtgaaggt cgacgcgtac
      361 gtcctcgatt tcgccgcgcg cgagatggaa gacgccgacc tcctccgcaa gctgcccgcc
      421 gataagaagg tccacgtcgg cgtgatcgac gtgcgcatgc tcgagatcga acatcccgag
      481 caagtcgccg agcgagtgcg caaggtgctg aagcacatcg aaccagagcg cgtcacgctc
      541 acgacggact gcggcctcaa gcagctgccg cgcgtcgtcg ccgtgcagaa gctgcgcgcg
      601 ctcgtcgagg gtgcagcgat cgttcgccgc gagctccgct agtcggagtg aatgcgcacg
      661 atccactcga tcgcgataac ggcgccctgc gccgcgaatc cataggagcc cgacgagatg
      721 gcgggtagcg tgccgcccac ggtcggcgcc gccgtattcc attgcgtcaa acacgtcgct
      781 tgcgtaccgc gctgatcgag cgagagacgg aagtctgcat gttcgagcgg cagggctgcg
      841 cctgtctgct cgacgaacga gtacatgccg ctcgatacgt cgagcgagta cgtgagtgcg
      901 aagtacccgc tcgtatgagg aacgatcgag ctgagctcgc aaaagtacac gtcgctgctc
      961 gcctggttcg gtctcgcgaa gatggacacc tggtgctggt gcgttggctc cgacgcaccg
     1021 aggtggccac cgatctcgac gcgatcggcg gtcaagactc cggcttgata gaccttcacc
     1081 ggagcgaacc gcggatccga gagcagtgag tcgttctgca gcgtcgcggc cacgctcgtg
     1141 gggtcgagca tccagccacc gaggctcgtc gaaaatgcgt cgaactggac gatgtgctcg
     1201 cgggcttccg ccggagctgg atcacacgca tcgccgaccc catcgccgtc gccgttctcc
     1261 tgctgcggat tcggaatgtg cgggcagttg tcgcacgcgt catcgatgcc gtcggtgtct
     1321 tcgtcgtgat ttgcggcgat cggacagagc gcgctgccgc tgtcgccgac ttcgtcgaac
     1381 gccatgcgcc cgcagccagc caacgcgaca acgaacaaca gacgcacgta gcgatcttac
     1441 tccggctgca gcacgaacca gtgaaacacc cggtagatgc cacttgcctc gacgcggatc
     1501 tcgctggcgg cctgtgggag cgcgtgcgct tgcacgttga acgtcagctc cgagaactgg
     1561 ccggtcggcg gcgcgacggt cagctcgccg agcttcttcg tgccgctcca gagcgtcatc
     1621 tgcacgggct tcgtgatctg ctcgtgccac ggatacgtgg gctggccgcc ggtgcggatc
     1681 acgatacgcg tcggcttgtc cggcacgacg tggacggtga acgtctcgcc gccgtcgcgg
     1741 atcgtgcgcc cgccgtcgat gagcaggcca tgtgcagtct ctttctcgac gacggaccac
     1801 cgcgcagtcg ggtcgccgaa cttgcgccgt cccattttgc cgacccagtc gtgcgcgcgc
     1861 tcgctcgcga tgtcggcgac gtcgagccga tcgacgaggg cccagcccgt gtgatcgttg
     1921 agcggccgct cggcggtgcc ggcgtgatcc cagacggcgg taaggatctg catatcgcct
     1981 tcgccgacga gccggcgccc gcttggatcg atgcccctcc gcagcgacgt gtgcatgagc
     2041 gcctcgccgt agaactccgg cgtgcccatc catcccgggt aatacgcgaa gtgcgtcggg
     2101 cgccggtcag gcgggatgct ctcgagaaac tcgaagcgcg cgcccggacc gttgttcgcg
     2161 atgcccgcct gatgattcgt gacgaggccc agcatgtcgt agacctcgcc gtcgccgtag
     2221 tacgcgatcg cgccggcatc gtggaacatc acgcgtgcgt cgggcagctt gcgatggagg
     2281 tagttgccga tcgcgacgac ctgcgtgttc gtgtccatcg caccctgcgc gaacaggcgc
     2341 atgtcggcga cgagcggctt gcgcgcggcg agaatgaaga acacgacgag caccgacgac
     2401 gctgcaaccc aaccgcgacg aatccaccgc cgatacggtg tgagcgctgc gggcagcgaa
     2461 tcgggcggcg cgaacgcgaa accgacggga atgaatagca gcggcagcgc cggcgcgatg
     2521 tagcgatagt tctggaagct ccacaggccc gacgaagcga cgaccgacat catcatcatg
     2581 aacggcgcca ggatcacgag cgcaccgacg agaaacttct tctcgcgcca cgcccaccac
     2641 gcgatgcgcg ccgagccgaa caggaacagc gcgagcacga cgcggggcca gagcagcggc
     2701 gatgtcttct cccagaacag gccgcggaac atcttcttcg tgagatcgac gacggtcagc
     2761 ttccagtaca cccagtcgaa gcctggcaga tagaagtggc tcttcgcgac gcccgtattc
     2821 gggaagaagt tgccggcgat gagcctgttc gcaagcagcc acacgatcgg cggcacgagc
     2881 ggcaggagcc accacgcggc aggcttccat gcgcgcttgc gcagacgcgc gagcaccgcg
     2941 atgccgacga tcgcaccgac gaggagcatt gcttccgggc gcgacaatga cgcggctgcg
     3001 agacacgcga cgagccgctt cgtcggtggc ccgtccgccg attgatcgac gagcagcaac
     3061 agcgatgcaa cgagcagcgc cgccgcaaac gcgacctcca ttccggacag cgcgcagaac
     3121 gcgaacggcg ggatcacgag cagcat
//
LOCUS       DATLBZ010000411        26633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7700922, whole genome shotgun sequence.
ACCESSION   DATLBZ010000411 DATLBZ010000000
VERSION     DATLBZ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 26633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26633
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7700922"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /locus_tag="VMZ53_20715"
     CDS             complement(<1..2323)
                     /locus_tag="VMZ53_20715"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HUS30950.1"
                     /translation="MSESPVLRSELERVTQALLHAVDALAGDPNEQLYIRLEEVATKL
                     RRHEVPRSELAAAVAKLDEEQLEAMARANALECHLMNTAEERERLRTLHTRGAALGDG
                     IAAAIDELLAAGLGETEMRALFDRALVMPVITAHPTESRRRSALDHLSRVHRLLDDVG
                     PRATAALEAEVLALHSTEDSRANRPTPLDEVETALDVFRRSLLEVTPRVYRTIEEKLE
                     QKLGGTWRLPTFLRWGTWVGGDRDGNPNVTAIVTRIALQRQRAVVLSRYVADVEALGK
                     SLSMSSLRARGSLDELIASVEADRERFPEVAAHARPRTVNEPWREKLWYVQARLRGTL
                     DHAEHGYPDDRRYRTDLAVLDRSLRAAGFAAIAEQDLRDMLRRVDVFGFHLASLDLRQ
                     HSGVHDRVVGELLERGGRAGYLDADEEGRRAMLRDILTKTITPVRDREGLSPDARELL
                     WTLDVVGRARRELGPRACERYITSFTREVSDLLEVVFLARAAGLAPGELRPVPLLEQL
                     EDLERSEQIAGALLGDPILRTEIGDELEVMVGYSDSGKQVGYVASSMALRRAQQVLAD
                     VAQNAGVKLTVFHGRGGALGRGGGPASDAIRAQPPQAVRGRMRVTEQGETVTARYTQP
                     AIAERDLELTIGAVLAAVAAERAAVPAEAASDEEHLAHAADAARQAYLELTSDEDRLA
                     RYTVAATPIEDVAHLPIGSRPASRAAKLTLESLRAIPWVFSWTQSRHGIPGWFGVGTA
                     VEHLVREMGDDAFRSLAQRSRFFRALVRNCEVSL"
     gene            2368..2724
                     /locus_tag="VMZ53_20720"
     CDS             2368..2724
                     /locus_tag="VMZ53_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HUS30951.1"
                     /translation="MPVSTHKGGCHCGAVRYSVEVDPSQESIACNCSMCGRAGSYLQF
                     VPEDKFTLEKGEDNLSDYQFNKHVIHHLFCKTCGIKSFARGVSPRGPTVAINMRCLDG
                     VNSFDLPTKQFDGASR"
     gene            complement(2726..4597)
                     /locus_tag="VMZ53_20725"
     CDS             complement(2726..4597)
                     /locus_tag="VMZ53_20725"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HUS30952.1"
                     /translation="MATAEAIAAAAPALTMAQLLELHPWPSQFKDEKKIERLWIYDLP
                     GTPQALWPYISDTSRMNRALGTAEMTFWEKDGKRYGSSKAGGVRHEWFEVPWNWVSEQ
                     WLTSLRIYERGFMKVNYAIHRLEPIATGTRLYLYFGVVPRGALYAAAIRIGFPTIKRA
                     YDRVLPQLAAQLDRLRPEVLQLPPPALSEEALARLDAIKQQLLGEGMPKESVEKLVDW
                     IRTGDDADLHRIQIRERARTWKVPEIDLLRTALHATRAGLLDLSWDTICPHCRGVRDE
                     TGKLSQLKAEGHCEVCQIHFGTDTPEAVEVTFRVHSSVREVPVQLYCSAEPARKDHIR
                     MQIAVAPGVEAKLQPRLTPGRYTVWAGHRGGWYLDVEDDGPSDLVYEDHPEGTVVRAS
                     PTPTVKFTNDGTEEEMFTIERVTWSDDALRPGMLLSFQEFRDLFSEDYIGADVKLAVG
                     EQTLLFTDVVGSTAFYASRGDPAAFIEIKKHFDEVFDIVAKNRGAIVKTIGDAVMATF
                     VNPVDAVRASQQIHNAFHPKREDTPIRLRISLNTGPCIAVRLNANADFFGGTVNIAAK
                     LQAMAEGYQIAMSEATYRSAGVPDLLTEQKADVEELIYESKALKEAVKVRRWSVYRE"
     gene            4623..6548
                     /locus_tag="VMZ53_20730"
     CDS             4623..6548
                     /locus_tag="VMZ53_20730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS30953.1"
                     /translation="MQDQLSAVVDAQMRFEMLFANSVDGVFFMTLDEPMRWSADADRE
                     ALLDYAFDHMRVASCNPTMCSQMGAARETLIGSSPRDRWKSDADRRQWREKMTTLFDQ
                     GRAAHRSRAPRDDGSYIDVEGSYVCLYDDEGRITGYFGTQRDVSAHRLATERLELAML
                     SGEIGIWEYDLVAGTLYFDDSWLKRLGYTEMIGKTHPATWWLEVMHSGDVDEVKRVFM
                     EHLEGKTPLYRCEHRVKTSDGRWLCLLSSGRVSSRDRDGRPLRFVGTSLDITERKQLQ
                     ERVALAERMASIGTLAAGVAHEINNPLTYIVLNLTLLERQLGALAGVEPETAAKLKNM
                     VSQARYGTERVSNVVRDLQSLTRVKDEPNTLVDPNAVLERCFEIADHQIRHRARLVRE
                     LSPLPAVRASEGRLVQLFLNLVVNAAHAIPEGNADQHTLKVRSSFVDGRVRVEVSDTG
                     AGIASDALGRIFDPFFTTKEIGEGSGLGLAICRSIVTSLGGDIEVESEVGKGSTFVVT
                     LPVGSASSDSTAPAAAKVAGGLRILVVDDEPLIGKVVEQVLVGHRVVSETSARAALAR
                     LQAGEIFDRILCDLMMPDMSGIELFNQLPAPVQKQVVFLSGGAFTERARDFLARVPNR
                     RLEKPFDTDALAAALAD"
     gene            6563..8683
                     /locus_tag="VMZ53_20735"
     CDS             6563..8683
                     /locus_tag="VMZ53_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HUS30954.1"
                     /translation="MTTTHFRSCPLCEASCGVEITVEGDRVTSVRGDEADPLSKGYIC
                     PKGTALADLHHDPDRLTKPMRRDGDTWHEMEWDEALDYVAARIRDVRTRYGKNAVAVY
                     QGNPTAHNLGLMTYGQLLFRTLGTKNTYSATSLDQLPHMLAALQMFGNQLLMAVPDVD
                     RADLFICLGANPLASNGSIMTAPDMRGRLKAIQDRGGKVIVIDPRRTETAERADQHLF
                     IRPGTDAVLLLAMINELFATQQVRLGRLAATGLETLRAISTPWTPERAAEITGIAAAD
                     IRALTNALATTEHAVLYGRIGVCVQEFGGLAAYLYYAVNALTRHLDEPGGLMFTTPAV
                     DPLPLAELLGFDGGFGRWKSRVSGQPEFGGEFPMSVLAEEITTPGEGQIRALITSAGN
                     PVLSAPGGPALEKAFGQLEFMVSIDPYMNETTRLAHVILPPTSALQRAHYDIALNAFA
                     VRNVAKYSPPLFPRCDDERHDWEICAGLFARLSVPVGRELAAKVLGKLGPETLLDLAL
                     RTGPHGLRSKGLSGLSLSKLRAAPHGIDLGALESRLPKRLSTKDRKLQLAPKLYIDDV
                     SRLEKTFSSAPAGLVMIGRRHLRSNNSWMHNSERLVKGPPRCTLMIHPDDAASRGLVD
                     GGNARVATSKGAIELPVEVTDTVMKGVVSVPHGWGHNKRGARLSVASTVPGVSVNDII
                     DPARIDVLSGTSALSGQPVEVTRA"
     gene            8680..9171
                     /locus_tag="VMZ53_20740"
     CDS             8680..9171
                     /locus_tag="VMZ53_20740"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HUS30955.1"
                     /translation="MTGQAIARGPELFARIGEQKLREVITDFYSRVFGDVMIGFMFEG
                     KNRQHLIDREYEFTASLLGGDVKYTGRPIRTAHAKTPIFGGHFERRLQILRETLRDQA
                     VDPDVQKVWIDHTLALRDQITADKGSECKDTSVVAPPEGAPLGVASPGPDPDARIKLG
                     KKR"
     gene            9182..9958
                     /locus_tag="VMZ53_20745"
     CDS             9182..9958
                     /locus_tag="VMZ53_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30956.1"
                     /translation="MRHAWLMLVLVGAAACARASGGQSDAAPGDTVIVDGAVDANNCS
                     SQPCSILPQCGCGGANACDIDVSDNKGTTCRAVATPGTETAACNGVTQCDKGYVCLGG
                     SAFASCKKYCTADADCGSPRGACVIDITANGTPLMGIPSVCSSNCDPQSTNSPECPST
                     YKCGLFNATHNGTQVRIADCSPAGTGTQGASCASGTMGADNLCAKGYLCSTITTDTAF
                     KCRRICKPNEVPTGCGAAQCLKFNPVFTIGTPPVEYGICN"
     gene            complement(9955..11637)
                     /locus_tag="VMZ53_20750"
     CDS             complement(9955..11637)
                     /locus_tag="VMZ53_20750"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HUS30957.1"
                     /translation="MTLLVLGAIGAVQAGLAGGRFMFDLEQDHPALSNAAPVLPDPHT
                     PRLARRVFVVIVDGLRLDKSYEQPFLDDLRRRGVDSEAMSHYPTWSRPNYVSILTGVP
                     PSASGVRTNHHSTPVALDSLMDRAHAAGLHTAMGSDYDVLPRLFLRPRRARGDVVKEP
                     AQPLQIDPSDVDGALDQESALAVQAPDANLESPFDDARYAPWPGGFSEAGDALAGADD
                     ELVVLLLGAVDAAGHAHGGNSDEYRAAVAIADHALGRALARVDLSQDAIIITADHGHT
                     GRGGHGGVEPEVMYVPLIAAGAGIKPGATVNDARLIDIAPTVSTLLGMPSPGHALGRS
                     LVEILDIDEPNANARRLADQQRMAVTGAIVAGAEARAAVDVLANRALRIAAVVGGAIL
                     AIVLATVLFKRRVLRFDIRAMLVSVPAFFVVYYALIGAVGQRFSPSLLPAQGHLAWVM
                     AKYGIIAMVVQLLFNLWALRSQKTLPDRLATANGIALVGLMLAMIPAGLAWAFFPPPY
                     TSVPGPVWLVLIPAVEVAVACAAIDVAVTLMVEVIVFAARAYYRRPPGTTLS"
     gene            11875..13296
                     /locus_tag="VMZ53_20755"
     CDS             11875..13296
                     /locus_tag="VMZ53_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HUS30958.1"
                     /translation="MDQLKTIGRSAPDAEPIQHLKPHVDPSTLQHRNLLDGPFWQRIP
                     AYRTIDEATFLDHTWQAKNTITNPAKLLAAVQDLVPKSFIDDVEQGFHRSPMSIRVSP
                     YLLSLIDWADPYSDPLRTQFIPIASRLLPDHPKLTLDSLHEQADAPVPGLTHRYPDKA
                     LFLALDTCPVYCRFCTRSYAVGVDTDVVDKVSLKATEDRWEQAFTYISERAELEDIVV
                     SGGDSYQLKARQITLIGNRLLEMPNIRRIRFATKGPAVMPMKLITDTEWLDALSSVVE
                     RGRKLHKEVVLHTHFNHPNEITAITKRALDRVFERGIITRNQSVLQRGVNDRVETMQL
                     LVKRLGHCQVHPYYVYVHDLVKGVEDLRTTLQTALDIEKAVRGVTAGFHTPTFVVDAP
                     GGGGKRVAHSFEHYDRETGISVFAAPSVKPGFFLYYDPVDTLSPEVQARWKDAAEQDR
                     MVEAAIAQAKANQVASSRASAVR"
     gene            13378..14208
                     /locus_tag="VMZ53_20760"
     CDS             13378..14208
                     /locus_tag="VMZ53_20760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30959.1"
                     /translation="MRRSLRSLLWTALGIGFVTACASDVYIGQRSDGVTVYCNKGEYY
                     YLCTPDYPELTDGSDSAQPGGGGDTSCNPDTYACPDGSGSGSGSGSNDGGGGGGGGGG
                     GSGSGSGSGSGSDDGDGGGGGGGGGGGGGGGGGGGGGGDGGGDQCQPWPVGTATILWP
                     PNHKMHTYTLNDCAKVDVCPNGQAIADGTVTKITVDEEVDVGKGGDGHTTKYDVSIVD
                     DTHFELRSERQGGMDGRVYTVYYVDGNGTEGSCQFLVPHNRGPHEGAVDSGTKVTVTP
                     "
     gene            complement(14205..15191)
                     /locus_tag="VMZ53_20765"
     CDS             complement(14205..15191)
                     /locus_tag="VMZ53_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30960.1"
                     /translation="MGRFEPTGPVDVPGLEHSVANLSWGVLRGALGPSDGTAGAASNV
                     PSALGVLRHADIYKGVPDEIEEAFAVLERHVVRDGQLYPVVVAALPFLFATLRKGTFI
                     ATRIADLIALYASTAHTLEAPLDTRVRQLISDHGGEIARWLGHHDRALGAIAIHVPAL
                     RDIFIAAVEGAERVSPEILLALVELGDAPGETPELALKMFDGPDASDIARTCAAAFLA
                     RFGDHAPELRTRIDAALPPSATTHLRRLAGNLWNPVIVRPLVAPKLYDAEILFTGKKV
                     VVVKAGSKSVTLPWEGAALVQGDRVQVGLSAHGQPKLAVVTDRKGNVRVIDF"
     gene            complement(15273..16664)
                     /locus_tag="VMZ53_20770"
     CDS             complement(15273..16664)
                     /locus_tag="VMZ53_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30961.1"
                     /translation="MKAVVAGVVTVASFAGCVGFDAREGKESQGDQKIVAGAGYTTFD
                     DALGGCKNGNGVNCNSYIGKDYVFMSGGPQHNVSGLADGSYFFAVVAPGAQATFFSGE
                     GLLSTDSVANRTFTTANHQVDLLAYDGPHAEGTSPQGRAILGLAPFADTPNNGGVYIL
                     AICLSGATSPSQCKYDAFRVRNGADPSEPQFPVVSGGKYYDANINGKWDSGEPGIGGW
                     PIDYHDEVSGTVATEADGKFTLTLIADTYLFVERQAPNSAWHQTGNTVDQTSVTGAAT
                     ATLSAKQYTVVAVDNSTVNGLYFGNVCVGAGGGHTLGFWSNKNGQSLIDAGDLQALRE
                     QNLYTWPSTAFDPTTKQQVRSWLLNGTATNMAIMLSVQMAATVLNIRNNLVANGAIVY
                     APGTTSADANGFASLTALIAEANVTLGENAYVLSGHPERAHQEALKNAFDKANNNLSF
                     LQAGPSTCSAPGF"
     gene            16847..17704
                     /locus_tag="VMZ53_20775"
     CDS             16847..17704
                     /locus_tag="VMZ53_20775"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS30962.1"
                     /translation="MRRSSIRELPFKHNQKPRLGFEIFKKSELFARQPEADLVAPKRL
                     DFHIICVGLRGRGTTIVDFQEVPLGAGHMTIVARGRVHNFLPKPGVDAWLLLARPEVV
                     TGTYALLDPMWMPPAVELSRDDRDELLGLCDQLAAEQTRPLDAIQPRLMEALLSAVLL
                     RGERLVHASASHQPVAPVLQRFFTILEADCLGTREVAHYAKRAGISVRQLSDLLVAHT
                     GKSTKRVIDERVVLEQKRLLAHTDVSVKELAERTGFDEPTNLVKFFRHHTGTTPQAFR
                     RNLPSGRRS"
     gene            17742..18611
                     /locus_tag="VMZ53_20780"
     CDS             17742..18611
                     /locus_tag="VMZ53_20780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS30963.1"
                     /translation="MSKTLLASVIALAAVAAPAAADTKLTATPYTATPTGFLVNSTLV
                     SGEKEAILIDGQFSLADAHRLVAVIMESKKTLTTVYVTHSHPDHYFGLNVIKQAFPKV
                     KIVATPAVVKEIQASWKGKVKQWGPMYGALVPTQPVIPTPLKGTTLTLEGQTLEIKQG
                     VGDAPDTTYVWIPSIKTVVAGDIVFRGVHPWTAEGKAEGRKAWIKTLDEISALNPEVV
                     IAGHKDPKLDDKPAGVKQTRDYLEAFDAAVASSKSAAEVQEKMKKKYGDLQLDVILQI
                     GAGAQFPQPAATK"
     gene            complement(18612..19292)
                     /locus_tag="VMZ53_20785"
     CDS             complement(18612..19292)
                     /locus_tag="VMZ53_20785"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HUS30964.1"
                     /translation="MLKTFRLGTFLGFPVDIKPSFVVLLGLVLIGFGGVTGVALMLLV
                     FASVVLHELGHAVAARRVGVHVSGIDLGFLGGAAKMQSLPRNARDEVIIAAAGPAVSL
                     ALGALGVGLGAITHVWLFAWLGWANFILAAFNLIPALPMDGGRILRALLTRKMDYVKA
                     TDTAVTVARVIAIAFVVIGIMGPYQLILLAPFLWIMGTREKMIARMTQDNYLYSPSGY
                     HFSPRWRL"
     gene            19448..19957
                     /locus_tag="VMZ53_20790"
     CDS             19448..19957
                     /locus_tag="VMZ53_20790"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS30965.1"
                     /translation="MKRLGFLAVLALATTAPITIQGAAAEPRHMSQNPRVGLLAELTF
                     DAGKLRLPDASGSQLGRVAAWAEENFDGLVVIDGHADRQGAKADNVRLALQRARLVRD
                     QLVGLGVDPDQIIISAFAAETHRQRARVAIWGTRNSREQVIGSRRKAHAIYNGNVSAR
                     QSPTLAGRR"
     gene            complement(19954..20538)
                     /locus_tag="VMZ53_20795"
     CDS             complement(19954..20538)
                     /locus_tag="VMZ53_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30966.1"
                     /translation="MRSAALAFVVVALVACSNKKSGPPPRCVVKVTLPALDADAAKAP
                     ACSATIIAPESTSYADVIAAMDTLAAKGFTDFGIGAVTFNLPAPPKSVPASRTTNEGK
                     IIGRIELADRAPSIVIGKTGEVSVGGNLVGNMSVPGGGKLDAALTAALPKPGPDAVII
                     ITAHASITYAAIERAAKAAAKAGYAGMLFSILMS"
     gene            complement(20553..21314)
                     /locus_tag="VMZ53_20800"
     CDS             complement(20553..21314)
                     /locus_tag="VMZ53_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30967.1"
                     /translation="MAYRGDDAGDALDAPTADGVLKATLGPNRLDMVLGSRTLHIADG
                     VATIIEAKKQKQGRRASHKIVGPIAVVRGWPRESFGLWIELAEEPLEKKRKPREPLPM
                     QRIFGVEPPNLWEFAGLVALGKLDSLAQRVRAHVEGFSQVLRGVEIGSGHPLDKVLVA
                     DDGERYVIYSRKLFRDRARFTMSVGADGKIVVPEGKGTKIVEVTDKFGVTVRGDYVRF
                     ADRHGTDLARVSVPWIGPEDRDELARRIGQLVHRA"
     gene            complement(21318..22244)
                     /locus_tag="VMZ53_20805"
     CDS             complement(21318..22244)
                     /locus_tag="VMZ53_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30968.1"
                     /translation="MTAADATCGSEAAAAGLPGTFVAFLTSSSVPDVRARFAGSRGWV
                     LVDGTPVGTTMDAMLDQYAVLNAVDMLADGSRFASNQSAWTGMLEDGKVDTTATCNDW
                     TTSSAAASGQGNVLRNATLAFGNPLPCDMPRHLICFEVGHRAEVAPIVSSGRLAFLTV
                     AKRTPSTNVAAADALCNAEATAAGFPGTYLAALNTSTASIASRFVVDQRPWVRVDGTL
                     ASDPSAILTGGLLRSFVHQASDGSYVRRGFYTGVSSTPNVTSAQTCMDWTSGNAMTNV
                     PIGLPPHILGSQFWSWSTSGCDAQLSVLCMQQ"
     gene            22437..24131
                     /gene="ettA"
                     /locus_tag="VMZ53_20810"
     CDS             22437..24131
                     /gene="ettA"
                     /locus_tag="VMZ53_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HUS30969.1"
                     /translation="MTAQYTFVMKDLRKLVPPKREILKGIWLSFYPGAKIGVLGNNGA
                     GKSTLLRIMAGVDKDFLGEAWPAPGLKVGYLAQEPQLDPTKTVKENVDEGVADTRALL
                     QRFEEVSMKLGEDMSEDKMNKLLEEQGRLQDQIEAVNAWELDHQVDMAMDALRCPPGD
                     ADVTKISGGEKRRVALCRLLLSNPDMLILDEPTNHLDAESVAWLEKTLETFKGTVVAV
                     THDRYFLDNVAKWILELDRGEGHPFEGNYSGWLELKAKRLKDEERQADARQKMLAREL
                     EWVRSSPKARQAKSKARLNRYDEMVAEAQKDARADGLEIVIAPGPRLGGEVVVFEGVS
                     KSFGDRLLIDNLSFKLPRGGIVGVIGPNGAGKTTLFRMIMGVEKPDKGTIKIGETVKL
                     GYVDQSRDALDDTKTLYDEIAEGAETLTLGEPPNVRTLNARAYVSSFNFKGQDQQKRV
                     GILSGGERNRVHLAKMIKKAGNVLLLDEPSNDLDVDTLRALEDGLEKFPGCAVVISHD
                     RWFLDRIATHILAFEGDSHVEFFEGNFQDYEADRKRRLGDAADQPHRIKYKPIRVS"
     gene            complement(24135..24917)
                     /locus_tag="VMZ53_20815"
     CDS             complement(24135..24917)
                     /locus_tag="VMZ53_20815"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HUS30970.1"
                     /translation="MKLRIVSYNIHKCIGGVDRRYEPERTAEVLRKLEADVLLLQEVD
                     AGVSRSKGDKQTEVLGEMVGMRYRCWYPNVDVRGGGQYGNAILSRYPLIESTNIDLTW
                     RFKKRRSALHGVIRVRHDDIDRTVHVYNMHLGLARFERRIQLRTFLDSHPFANLHAET
                     PIVVGGDLNDVYGGLSELLKPAGFRGIERRPLTFPAWGPVRPLDAIFVRGNVDFMKLS
                     RCDSELAKRASDHRPLVAEVRLHAHHPHHAHHHDHKPPSPRP"
     gene            complement(24914..>26633)
                     /locus_tag="VMZ53_20820"
     CDS             complement(24914..>26633)
                     /locus_tag="VMZ53_20820"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HUS30971.1"
                     /translation="NAGSLTWLILLTTVLNATPAFIEFVLHVKNMKAGAERRQLRLML
                     AASFITYIGLTDVAFVYDIGIFPLGWLLSGLGSVLVVRALVVEDLLRVRAIDTTAPRL
                     VFHFAVALLLGWVSLRMLGSAIPWWLATAVLAVSFAGVRTTFAIVSLINRGVRGSQST
                     LDRLLAQLVTRSRPLESEGKIAQLAVDVVELGVGARVEVLLAAAEDYGWATADGQKLA
                     DDAAPDPLLGAWLAENRTPLFRDELGDVPADLHDLLNGLFDKRNAIALVPVTNNDELL
                     AIVALPANARRVRGRELAFLQSAAERLGEALVHARMAQRAAQRAQIAREVELAATVQA
                     QLLPGPGPHVHGDITVIGSWLPAARCAGDFWCVYPIGEKRVLVAIGDVTGHGVPSAMV
                     TAGASAAVDVCVRKYQAQLALPDLIAALDAAVRRVGGGQLNMTCFAAILDPDALEIRF
                     VSCGHTTPYLVRADGEIELQALVGRGNPLGGSSSSLPKVLQKPMRAGDLVVWYTDGVI
                     EAQDSVGEPFGDRRLQRMLRRLERQSLNPPGVHHLVYAGVAAHRGGKPRGDDETLVVA
                     QWKPHS"
BASE COUNT         4741 a         8722 c         8773 g         4397 t
ORIGIN      
        1 cgagcgagac ctcgcagttg cgcacgagag cgcggaagaa gcgcgagcgc tgggccaggc
       61 tgcgaaacgc gtcgtcgccc atctcgcgca cgaggtgctc caccgccgtg ccgacgccga
      121 accaacccgg aatgccgtgg cgcgattgcg tccacgagaa gacccatgga atggcgcgca
      181 gtgattcgag cgtgagcttt gcggcacgtg atgcgggacg cgaaccgatc ggcaaatgcg
      241 cgacgtcttc gatcggtgtg gccgcgaccg tgtagcgcgc gagacgatct tcgtcgctgg
      301 tgagctcgag atacgcctgg cgtgccgcat ccgcggcgtg agcgagatgc tcttcgtcgc
      361 tcgcagcttc ggcgggtact gctgcgcgct ctgctgccac cgcggcgagc acggcgccga
      421 tcgtgagctc caagtcgcgc tcggcgatcg ccggctgcgt gtagcgcgcg gttacagtct
      481 cgccctgttc ggtcacgcgc atgcggccgc gcactgcttg cggcggttgc gcgcggatcg
      541 catcactcgc cggtccaccg ccgcggccga gtgcgccacc tcgaccgtgg aacaccgtca
      601 acttgacgcc ggcgttctgc gcgacatcgg cgaggacctg ctgcgcgcga cgcagtgcca
      661 tcgacgacgc gacatagccg acctgcttgc cggagtcgga gtagccgacc atcacctcga
      721 gctcgtcgcc gatctcggtc cgcaagatgg gatcgccgag cagcgcgccc gcgatctgtt
      781 ccgagcgctc gagatcctcg agttgctcca gcagcggtac cggacgaagc tcgccgggcg
      841 cgagacccgc agcgcgcgcg aggaacacga cctcgagcaa gtccgagacc tcgcgcgtga
      901 acgacgtgat gtagcgctcg catgcacgcg gtccgagctc gcgacgtgca cggccgacga
      961 cgtcgagtgt ccacagcagc tcgcgtgcgt ccggcgacaa tccctcgcgg tcgcgcaccg
     1021 gcgtgatcgt cttcgtgagg atgtcgcgca gcatcgcgcg gcgaccttct tcgtcggcgt
     1081 cgaggtaccc cgcgcgcccg ccgcgctcca gcaattctcc gacgacgcga tcgtgcacgc
     1141 cggagtgctg ccgcagatcg agcgacgcga gatgaaagcc gaacacgtcg acgcggcgaa
     1201 gcatgtcgcg cagatcctgc tcggcgatcg ccgcgaagcc cgcagcgcgc agcgaccggt
     1261 cgagcaccgc gaggtccgtg cggtagcggc gatcgtccgg atagccgtgc tccgcgtgat
     1321 cgagcgtgcc gcgcagacgc gcctggacgt accagagctt ctcgcgccac ggctcgttga
     1381 ccgtgcgcgg ccgggcgtgc gccgcgactt caggaaaccg ctcgcgatcg gcttccacag
     1441 aagcaatgag ctcgtcgagc gatccgcgcg cacgcagcga cgacatcgac agcgacttgc
     1501 cgagcgcctc gacatccgcg acgtagcgcg agagcacgac agcccgctgc cgctgcaagg
     1561 cgatacgggt gacgatcgcg gtcacattcg gatttccgtc gcgatcgccg ccaacccacg
     1621 tgccccagcg cagaaacgtc gggaggcgcc aggtcccgcc gagcttctgc tcgagcttct
     1681 cttcgatcgt gcgatagacg cgcggcgtga cctcgagcag cgaccgccga aacacgtcga
     1741 gtgcggtctc gacctcgtcg aggggcgtcg ggcgattcgc gcgcgaatcc tctgtcgagt
     1801 gcagtgccag gacctcggct tcgagcgctg ccgtcgcgcg cgggccgacg tcgtcgagca
     1861 atcgatgcac gcgcgagaga tggtccagcg cggaccgtcg ccgcgactcc gtggggtgcg
     1921 ctgtgatcac gggcatgaca agcgcccggt cgaacagcgc gcgcatctcg gtctcgccca
     1981 accccgcggc aagcagctcg tcgatcgcgg cggcgattcc gtcgccgagc gccgcgccac
     2041 gcgtatgtaa cgtccgcagt cgctcgcgct cctccgccgt gttcatcaga tgacattcga
     2101 gcgcgttcgc gcgggccatc gcttcgagct gttcctcgtc gagcttcgcg actgccgcag
     2161 ccagctcgct tctcggcacc tcatggcgcc gcaacttcgt cgcgacttcc tcgagccgga
     2221 tgtacagctg ctcgttcgga tcgcccgcca acgcatctac tgcatggagc agcgcctgcg
     2281 ttacgcgttc gagctcgctc cggagtactg gcgactcgct cacgcgcggg ttctacctcg
     2341 aatcgacgac tggagtagtc tccacacatg ccagtctcca cccacaaagg cggttgccac
     2401 tgcggcgccg tccgttactc cgtcgaagtc gatccatccc aggagtcgat cgcctgcaat
     2461 tgctcgatgt gcggccgcgc gggcagctat ctgcaattcg tgcccgagga caagttcacg
     2521 ctcgagaagg gcgaggacaa cctgagcgac tatcagttca acaagcacgt catccatcac
     2581 ctcttctgca agacgtgcgg gatcaagtcg ttcgcccgcg gcgtatcccc gcgaggtccg
     2641 acggtggcga tcaacatgcg ctgcctcgac ggcgtgaact cgttcgacct gccgacgaaa
     2701 caattcgacg gcgcctcgcg ctgactcact cgcgatagac cgaccagcgc cggaccttga
     2761 ctgcttcttt gagcgccttc gactcgtaga tcagctcttc gacatcggcc ttttgctccg
     2821 tcaggaggtc gggcacaccg gccgagcggt acgtcgcttc actcatcgcg atttggtagc
     2881 cctcggccat cgcctgcagc ttcgccgcga tgttcacggt gcccccgaag aagtcggcat
     2941 tcgcgttcag gcgtacggcg atgcatggcc cggtgttgag cgagatgcgt aggcggatcg
     3001 gcgtgtcttc gcgtttcggg tggaacgcgt tgtggatctg ctgactcgcg cgcacggcgt
     3061 cgacgggatt gacgaacgtc gccatcaccg cgtcaccgat ggtcttcacg atcgcgccgc
     3121 ggttcttggc gacgatgtcg aacacctcgt cgaagtgctt cttgatctcg atgaatgcgg
     3181 ctgggtcgcc gcgcgacgcg tagaacgccg tcgagccgac gacgtcggtg aagagcagcg
     3241 tctgctcacc gaccgcgagc ttcacgtcgg caccgatgta gtcctcgctg aagaggtcgc
     3301 ggaattcttg gaacgagagc agcatccctg ggcgaagtgc gtcgtcactc cacgtcacgc
     3361 gctcgatcgt gaacatctcc tcctcggtgc cgtcgttggt gaacttcacc gtcggagttg
     3421 ggctcgcgcg caccacggtg ccctcgggat gatcttcgta gacgaggtcg gacgggccat
     3481 cgtcttcgac gtcgaggtac cagccgccgc ggtggcctgc ccacacggtg tagcggccgg
     3541 gcgtgagtcg cggttggagc ttcgcttcga cgcccggcgc gacggcaatc tgcatgcgga
     3601 tgtgatcttt gcgcgccggt tcggcactgc agtacagctg caccggcacc tcgcgcaccg
     3661 aggagtggac gcggaacgtg acctcgacgg cttcgggcgt atcggtgccg aaatggatct
     3721 ggcagacctc gcagtgacct tcggctttga gctgggagag cttgccggtt tcgtcgcgca
     3781 cgccgcggca gtgcgggcag atcgtgtccc acgatagatc gagcagcccg gcgcgcgtcg
     3841 cgtggagggc cgtgcgcagc agatcgatct cggggacctt ccacgtgcgc gcgcgttcgc
     3901 ggatctgaat gcgatggaga tcggcgtcgt cgccggtacg gatccagtcg acgagctttt
     3961 cgacggactc cttcggcatg ccttcgccga ggagctgctg cttgattgca tcgagtcgcg
     4021 cgagcgcttc ttcggagagc gcaggcggcg ggagttggag gacttcgggg cgaaggcgat
     4081 cgagctgcgc agcgagctgc gggagcacgc gatcgtatgc gcgcttgatc gtcgggaagc
     4141 cgatgcggat ggcggcagcg tagagagcgc cgcgcggcac gacgccgaag tagagataga
     4201 ggcgagttcc cgtcgcgatc ggttcgaggc ggtgaatcgc gtagttcacc ttcatgaagc
     4261 cgcgctcgta gatgcggagc gacgtgagcc actgctcgga gacccagttc cacggcactt
     4321 cgaaccactc gtgacgaacg cctcctgctt tcgacgagcc gtagcgcttt ccgtcttttt
     4381 cccagaacgt catctcggcg gtgccgagcg cgcgattcat ccgcgatgtg tcggagatgt
     4441 acggccagag cgcttgtggg gttccgggaa gatcgtagat ccagagccgc tcgatcttct
     4501 tctcgtcctt gaactgcgag ggccacggat ggagctcgag cagctgcgcc atcgtcaggg
     4561 caggggcagc ggcagcgatt gcttcggcgg tggccacgtc cacatggtac caattcacgg
     4621 ccgtgcagga tcagctcagc gccgtcgtcg acgcacagat gcgcttcgag atgctgtttg
     4681 ccaatagcgt cgatggggtg ttctttatga ccctcgacga gcccatgcgg tggtcggcag
     4741 atgcggatcg agaggcgctt ctcgactacg cgttcgacca catgcgcgtg gcgagctgta
     4801 accccaccat gtgctctcag atgggagccg cgcgcgagac gctcatcgga tcctcgccgc
     4861 gcgatcgctg gaagtcggat gcggatcgcc ggcagtggcg cgagaagatg acaacgctat
     4921 tcgatcaagg ccgcgccgca caccgctcgc gcgcgcctcg cgacgacggc agttacatcg
     4981 atgtcgaagg ctcctacgtg tgtctctacg acgacgaagg ccgtatcacc ggctacttcg
     5041 ggacacagcg cgacgtcagc gcgcatcgac tcgcgaccga gcgactcgag ctggcgatgc
     5101 tgtctggcga gatcgggatc tgggagtacg acctcgtcgc cggcacgttg tatttcgacg
     5161 acagctggct gaagcgtctc ggctacaccg agatgatcgg caagacccat cccgccacat
     5221 ggtggctcga ggtcatgcac tcgggtgacg tcgacgaggt gaagcgcgtc ttcatggagc
     5281 acctcgaggg taagacaccg ctctatcgct gcgagcatcg cgtcaagacg tcggacggtc
     5341 gctggctgtg cctgctgtcg agtgggcgtg tgtcgagccg cgatcgcgac ggcaggccgc
     5401 tgcgcttcgt cggaacgagc ctcgatatca ccgagcgcaa gcagctgcag gagcgcgtcg
     5461 cgctggccga gcgcatggcg tcgatcggca cgctcgcagc gggcgtcgca cacgagatca
     5521 ataatccgct cacgtacatc gtgctcaatt tgacgttgct cgagcgtcag ctgggcgcgc
     5581 tcgccggcgt cgagccagag acggccgcga agctgaagaa catggtgtcg caagctcgct
     5641 acggcaccga gcgagtcagc aatgtcgtgc gcgatctgca atcgctgaca cgcgtgaagg
     5701 acgaaccgaa tacgctcgtc gatccgaacg cggtgctcga gcgctgcttc gagatcgcgg
     5761 accatcagat ccgtcatcgc gcacgtctcg ttcgcgagct ctcgccgctc ccagcggtcc
     5821 gtgcgagtga gggccgcctc gtccagctgt tcctcaatct cgtcgtcaat gcggcgcacg
     5881 cgattcccga aggcaacgcc gatcagcaca cgctcaaggt ccgctcgtcg ttcgtcgacg
     5941 gtcgcgtgcg cgtcgaggtc agcgacaccg gcgctggcat tgcgtcggat gcactcggac
     6001 gcatcttcga tccgttcttc acgacaaagg agatcggcga aggcagcggg ctcggtctcg
     6061 cgatctgccg cagcatcgtc acgtcactcg gcggggatat cgaagtcgag agcgaggtcg
     6121 gcaaaggctc gacattcgtc gtgacgctcc ccgtcggcag cgcgagctcg gacagcaccg
     6181 cgcctgccgc agcgaaggtc gcgggcgggc tgcgaatcct cgtcgtcgac gacgaaccgc
     6241 tgatcggcaa ggtcgtcgag caggtcctcg tcggccatcg cgtcgtgagt gagacgtctg
     6301 cgcgagcagc cctcgctcga ctccaggccg gcgagatatt cgaccgcatc ctgtgcgacc
     6361 tgatgatgcc ggacatgtcg ggcatcgagc tgttcaatca gctgcccgcc ccggtccaga
     6421 aacaggtggt gttcctgagt ggtggcgcat tcaccgagcg cgcgcgggac tttctggctc
     6481 gcgtgccgaa ccgccggctc gagaagccgt tcgacaccga tgccctcgcc gcagcgctcg
     6541 ccgactgagc taagctagct cggtgacgac gacgcatttc cgcagctgtc cactctgtga
     6601 agcgagctgt ggtgtcgaga tcaccgtcga gggtgaccgc gtcacgagcg tacgcggcga
     6661 cgaagctgat ccgttgagca agggctacat ctgcccgaag ggcacggcgc tcgcggatct
     6721 gcatcacgat ccggatcggc tcacgaagcc gatgcgccgc gacggcgaca cgtggcacga
     6781 gatggagtgg gacgaagcgc tcgactacgt cgccgcacgc attcgcgacg ttcgtacgcg
     6841 ctacggcaag aacgcggtcg ccgtctatca gggcaatccg acggcacaca acctcgggct
     6901 catgacgtac ggccagctgc tgtttcgcac gctcggcacg aagaacacgt actcggcgac
     6961 gtcgctcgat cagctgccgc acatgctcgc ggccctgcag atgttcggca accagctgtt
     7021 gatggcggtg cccgacgtcg atcgcgcgga cctcttcatc tgcctcggtg caaatccgct
     7081 cgcgtcgaac ggcagcatca tgacggcgcc cgacatgcgc ggccggctca aggcgatcca
     7141 agatcgcggc ggcaaagtga tcgtgatcga tccgcgccgc accgagaccg ccgagcgcgc
     7201 cgatcagcat ctcttcattc gtcccggcac cgacgccgtg ctgctgctcg cgatgatcaa
     7261 cgagctgttc gcgacgcagc aagtgcgcct cggccggctc gccgcgacgg ggctcgaaac
     7321 gttgcgcgcg atctcgacgc cgtggacgcc cgagcgcgcc gccgagatca ccggcatcgc
     7381 cgccgcggat attcgcgcgc tcacgaacgc gctcgcgacg accgaacatg cggtgctcta
     7441 cggccgcatc ggcgtgtgcg ttcaggagtt cggcggcctc gccgcgtatc tgtattacgc
     7501 agtcaacgcg ctgacgcgtc acctcgacga gccgggcggc ctgatgttca cgacgccggc
     7561 agtcgatccg cttccgctcg ccgagctgct cggcttcgac ggtgggtttg gccggtggaa
     7621 gtcgcgcgtg tcggggcagc ccgagttcgg cggcgagttt ccgatgtccg tgctcgccga
     7681 ggagatcacg acgccgggcg aaggccagat tcgcgcgctc atcacgagcg ccggcaatcc
     7741 cgtgctgtcg gcaccgggcg gacccgcgct cgagaaggcg ttcggccagc tcgaattcat
     7801 ggtgtcgatc gatccgtaca tgaacgagac aacgcggctc gcgcacgtga tcttgccgcc
     7861 gacgtccgcg ctgcagcgcg cgcattacga catcgcgctc aacgcattcg ccgtgcgcaa
     7921 cgtcgccaag tactcgccgc cgctgttccc ccgttgcgac gacgagcgcc acgattggga
     7981 aatctgcgcg gggctgttcg cgcggctctc ggtgcccgtc ggtcgcgagc tcgcggcgaa
     8041 ggtgctcggc aagctcggcc ccgagacgct gctcgatctc gcacttcgca ccgggcccca
     8101 tggcctgcgc tcgaagggcc taagcggtct atcgctcagc aagctgcgcg cggcgccgca
     8161 tggcatcgac ctcggtgcgc tcgagtcgcg actgccgaag cggctcagca ccaaggatcg
     8221 caagctccag ctcgcaccga agctctatat cgacgacgtc agccggctcg agaagacatt
     8281 ctcgtcggcg ccggcaggcc tcgtgatgat cggccgccgt catctccgct ccaacaactc
     8341 gtggatgcac aacagcgagc gcctcgtgaa aggtccaccg cgctgcacgc tgatgatcca
     8401 tcccgacgac gctgcatcgc gtggtctcgt cgacggcggc aacgctcgcg tcgcgacatc
     8461 gaagggcgcg atcgagctgc ccgtcgaagt caccgacacc gtgatgaagg gtgtcgtctc
     8521 ggtgccgcac ggctggggac acaacaagcg cggcgctcgc ctctccgtcg catcgaccgt
     8581 gcccggcgtc agcgtcaacg acatcatcga tcccgcgcgc atcgacgtgc tgtcgggaac
     8641 gagcgcgctc tccggtcagc cggtcgaggt cacccgcgca tgaccggtca ggcgatcgcc
     8701 cgcggcccgg agctgttcgc caggatcggc gagcagaagt tgcgcgaggt gatcaccgac
     8761 ttctactcgc gcgtgttcgg cgacgtgatg atcggcttca tgttcgaggg caagaatcgc
     8821 cagcatctca tcgatcgcga gtacgagttc acggcgagcc tcctcggtgg cgacgtgaag
     8881 tacaccgggc gcccgatccg cacggcgcac gcgaagacgc cgatcttcgg cgggcatttc
     8941 gagcgccggc tccagatcct gcgcgagacc ctgcgcgatc aggcggtcga tcccgacgtc
     9001 cagaaagtgt ggatcgatca cacgctggcc ctgcgggacc agatcacggc ggacaaaggc
     9061 tcggagtgca aggacacgtc ggtcgtcgcg ccgcccgaag gggctcccct cggcgtcgcc
     9121 tcacccggtc cggatccgga cgcccgtatc aagctgggca aaaagcgcta ggattcggcc
     9181 catgcgccac gcttggctga tgctcgtgct cgtcggggca gcggcatgcg cgcgagcgtc
     9241 cggtggccag agtgatgcgg ccccgggcga cacggtgatc gtcgacggcg cggtcgatgc
     9301 gaacaactgt tcgtctcagc cgtgcagcat tcttccgcag tgcggatgcg gcggcgcaaa
     9361 cgcgtgcgac atcgacgtca gcgacaacaa aggcactaca tgccgtgccg tcgcgacgcc
     9421 gggcaccgag accgccgcgt gcaacggtgt cacgcaatgc gacaaaggct acgtgtgcct
     9481 tggcggtagc gcgttcgcat cctgcaagaa gtactgcacc gccgacgcgg actgcggctc
     9541 gccgcgcggt gcgtgcgtca tcgacatcac ggccaacggc acgccgctga tgggcatccc
     9601 gtcggtgtgc tcttccaact gcgatccgca gagcaccaat tcgcccgagt gcccgtcgac
     9661 gtacaagtgc ggcctgttca atgcgactca taacggaacg caagtccgca tcgccgactg
     9721 cagccccgcc ggaacgggca cgcaaggcgc gagctgcgca tccggcacga tgggcgcgga
     9781 caacctctgt gcgaagggct atctctgctc gacgatcacg acggatacag cgttcaagtg
     9841 ccgtcgcata tgcaagccga acgaagtgcc gacgggctgc ggcgctgcgc agtgcctgaa
     9901 gttcaatccc gtgttcacga tcggaacgcc gccggtcgaa tacgggatct gtaactagct
     9961 cagcgtcgtc ccgggcgggc ggcggtagta cgcgcgcgct gcgaacacga tcacttcgac
    10021 catcagcgtg acggcgacgt cgatcgccgc gcatgcgact gcgacctcga cggcgggaat
    10081 cagcacgagc cagaccgggc cgggcacgct cgtgtacggc ggcgggaaga atgcccatgc
    10141 cagaccggct ggaatcatcg cgagcatcag gccgacgagc gcgatgccgt tcgcggtcgc
    10201 gagtcgatcg ggcagcgtct tctggctgcg cagcgcccac aggttgaaca gcagctggac
    10261 gaccatcgcg atgatgccgt acttcgccat cacccaggcg aggtggcctt gcgcgggcag
    10321 cagcgagggc gagaagcgct ggccaactgc accgatcagc gcgtagtaca cgacgaagaa
    10381 cgccggcacc gagacgagca tcgcgcggat gtcgaagcgc agcacgcgac gcttgaagag
    10441 gaccgtcgcg aggacgatcg cgaggatcgc gccaccgacg accgcagcga tgcgcagcgc
    10501 gcgattcgcg agcacgtcga ccgctgcacg cgcttcggca cccgcgacga tcgcaccggt
    10561 gacggccatg cgctgttgat cggcgaggcg tcgcgcgttc gcattgggct catcgatgtc
    10621 gaggatctcg acgagcgagc gaccgagcgc gtggccgggc gatggcatcc ccagcagcgt
    10681 cgagacggtc ggcgcgatgt cgatcaagcg cgcgtcgttg acagtggcgc cgggcttgat
    10741 gccggcaccg gcggcgatga gcggcacgta catgacctcg ggctcgacgc cgccgtggcc
    10801 gccgcgaccg gtgtgaccgt gatccgcggt gatgatgatc gcgtcctgcg agagatcgac
    10861 gcgagcgagt gcacggccaa gcgcgtgatc ggcgatcgcg acggcggcgc gatactcgtc
    10921 gctgttaccg ccgtgcgcgt ggcctgctgc atcgacggcg ccgaggagca gcacgacgag
    10981 ctcgtcatcg gcgcctgcga gtgcgtcacc ggcttcggag aagccgccgg gccatggcgc
    11041 gtagcgtgcg tcgtcgaatg gcgactcgag gtttgcatcc ggcgcctgca cggcgagcgc
    11101 ggattcttga tcgagtgcgc cgtcgacatc ggacggatcg atttggagtg gttgggccgg
    11161 ctccttgacg acatcgccac gcgcgcggcg cggccggagg aacaggcgag gcagcacgtc
    11221 gtaatcgctg cccatcgcgg tgtggaggcc ggcggcgtgc gcgcgatcca tcagagaatc
    11281 tagggcgacc ggcgtcgagt gatggttcgt ccgcacgcca ctcgcgctcg gcggcacgcc
    11341 ggtcaggatc gacacgtagt tcgggcgcga ccacgtcggg tagtgcgaca tcgcctcgct
    11401 atcgacgccg cggcggcgaa ggtcatcgag gaacggctgc tcgtacgact tgtcgaggcg
    11461 aagaccgtcg acgatcacga caaacacgcg gcgcgcgagc cgaggcgtat gcggatccgg
    11521 cagcaccggc gcggcattcg agagggcggg gtggtcctgc tcgaggtcga acatgaagcg
    11581 gccgcccgcg agcccagcct gcacggcacc gatcgcgccg agcaccagca gggtcaacgc
    11641 cgccgctacc acctgccgag tcggcgccgt gtcgtgccgc atcagctgtt tcacccatga
    11701 ggttagcgcg gcggtcgtga aagggcatct cgacgcaatc gttagggcgt gataatcgtt
    11761 ggatcatcat gtggtacctg ggcaccgtcc cgcaccagcc cgacgtcgag tgaaaggccg
    11821 cgtttcgtcg acctttctgc ttcgccttct gggcccgctc gcgaggactt ttccatggac
    11881 caactgaaaa ctatcggtcg ctctgctccc gacgcagaac cgattcagca cctgaagccg
    11941 cacgtcgatc cctcgacgct gcagcaccgc aacctgctcg acggcccgtt ctggcaacgg
    12001 attccggcgt atcgcacgat cgacgaagcg accttcctcg atcacacctg gcaggcgaag
    12061 aacacgatca cgaatcccgc aaagctcctc gccgcggtcc aagacctcgt gccgaagagc
    12121 ttcatcgacg acgtcgagca gggctttcat cgctcgccga tgtcgatccg cgtctcgccc
    12181 tatctattgt cgctgatcga ctgggccgat ccgtatagcg atccgctgcg cacgcagttc
    12241 attccgatcg cgtcgcgcct cctgcccgat catccgaagc tcacgctcga ctcgctgcac
    12301 gagcaagcgg atgcgccggt gcctgggctc acgcatcgct atcccgacaa ggcgctgttc
    12361 ctcgcgctcg acacgtgccc ggtctattgc cggttctgca cgcgcagcta cgcggtcggc
    12421 gtcgacacgg atgtcgtcga caaggtctcg ctcaaggcga ccgaagatcg ttgggagcag
    12481 gcgttcacgt acatcagcga gcgcgccgag ctcgaagaca tcgtcgtctc cggcggcgac
    12541 agctatcagc tcaaggctcg ccagatcacg ctcatcggca accgtctact cgagatgccg
    12601 aacattcgcc gcattcgctt tgcgacgaag ggcccggccg tgatgccgat gaagctcatc
    12661 accgacaccg agtggctcga tgctctctcg tcggtcgtcg agcgcggccg caagctgcac
    12721 aaggaagtgg tccttcacac gcacttcaat cacccgaacg agatcaccgc gatcacgaag
    12781 cgcgctctcg atcgcgtatt cgagcgcggc atcatcacgc gcaaccagtc ggtgctccaa
    12841 cgcggcgtca acgatcgcgt cgagacgatg cagctcctcg tcaaacgcct cggccactgc
    12901 caagtgcacc cgtactacgt ctacgtgcac gacctcgtca aaggcgtcga ggatctgcgc
    12961 accacgctgc agacggcgct cgatatcgag aaagcagttc gcggcgtgac agccggcttc
    13021 cacaccccga cgttcgttgt cgatgcaccg ggtggtggcg gcaagcgcgt cgcgcacagc
    13081 ttcgagcact acgaccgcga gacgggcatc tcggtcttcg ccgcaccgtc ggtgaagcct
    13141 ggcttcttcc tctattacga tccggtcgac acgctctcgc ccgaggttca agcccgctgg
    13201 aaggacgccg ccgagcaaga tcgcatggtc gaagcggcga tcgcgcaggc caaggcgaac
    13261 caggtcgcca gctcccgcgc ttcggcagtc cgataaaaac gttggacgtc aaagcagtta
    13321 taaaatagct gccacctttc ggccgcgcct ccctacttac cggacatgga ggtgcacatg
    13381 cgacgctcgc ttcgctctct gctctggacc gcgctcggca tcggcttcgt gacggcctgc
    13441 gcgtcggacg tctatatcgg acagcgctcc gatggcgtga ccgtctactg caacaagggc
    13501 gagtactact acctgtgcac cccggactat ccagagctca ccgacggtag cgatagcgcg
    13561 cagcccggcg gcggcggcga tacgagctgc aatcccgata cgtatgcgtg ccccgacggc
    13621 agcggctccg gctcgggctc tggttccaat gacggtggtg gcggtggcgg cggcggtggc
    13681 ggcggctcag gttcgggctc cggctcgggc tccggatccg atgacggcga tggtggcggc
    13741 ggcggcggtg gcggcggcgg cggtggtggc ggcggtggtg gcggaggagg cggcggtgac
    13801 ggcggtggcg accaatgcca gccgtggccg gtcgggacag cgacgatcct gtggccaccg
    13861 aaccacaaga tgcacacgta cacgctgaac gactgcgcga aggtcgacgt gtgcccgaac
    13921 ggtcaagcga tcgccgacgg cacggtcacg aagatcaccg tcgatgaaga agtcgacgtc
    13981 ggcaaaggcg gcgacggcca cacgacgaag tacgacgtct cgatcgtcga cgacacgcac
    14041 ttcgagctac gcagcgagcg tcagggcggc atggacggcc gcgtctacac ggtctactac
    14101 gtcgacggca acggcaccga aggcagctgc cagttcctcg tcccgcacaa ccgcggcccg
    14161 cacgaaggcg cggtcgacag cggcacgaag gtgacggtca cgccctagaa gtcgatgacg
    14221 cgaacgttgc ccttgcgatc cgtcacgacg gcgagcttgg gctgaccatg cgcggagagg
    14281 ccgacctgca ctcggtcacc ttgcacgagc gcagcgcctt cccacggcag cgtcacgctc
    14341 ttggagccgg ccttcacgac gacgaccttc ttgcccgtga acaagatctc ggcgtcgtag
    14401 agcttcggcg cgacgagcgg gcgcacgatc actggattcc acagattgcc cgcgaggcgg
    14461 cgcagatgcg tcgtcgcgct cggcggtagc gcggcatcga tacgcgtgcg cagctccggt
    14521 gcgtgatcgc cgaaccgagc gaggaacgcc gccgcgcagg ttcgcgcgat gtcggacgcg
    14581 tccggaccgt cgaacatctt caaggcaagc tctggcgttt cgcctggggc atcaccgagc
    14641 tcgacgagcg cgagcaggat ctccggagac acgcgctcgg caccttcgac cgcggcgatg
    14701 aagatgtcgc gcagcgccgg gacatgaatc gcaatggcgc ccagcgcgcg atcgtgatgg
    14761 ccgagccagc gcgcgatctc gccgccgtga tcggagatga gctgccgcac gcgcgtatcg
    14821 agcggcgctt cgagcgtgtg cgccgtgctc gcgtagagcg cgatcagatc cgcaatgcga
    14881 gtggcgatga acgtgccctt gcgcagcgtg gcgaacagga acggcagcgc ggcgacgacg
    14941 acggggtaga gctggccatc gcgcacgacg tggcgctcga gaacggcgaa cgcttcctcg
    15001 atctcgtcgg ggacgccctt gtagatgtcg gcgtggcgca agacgccgag cgccgagggc
    15061 acgttcgacg cagcgcccgc cgtgccatca ctcgggccga gcgcgccgcg aagcacgccc
    15121 cacgagagat tggcaacgga atgctcgagg ccagggacat cgacaggtcc ggttggctcg
    15181 aatctcccca tgcgcgcgca ccataaggca attgacgcct cgctggcgag ctacaacttc
    15241 acgaccatgg tggttggacc acctgaagcg gactagaagc cgggtgccga gcaggtcgac
    15301 gggccggcct ggaggaagct caagttgttg ttcgctttgt cgaacgcgtt cttgagtgct
    15361 tcctggtgcg cgcgctccgg gtggcccgag aggacgtacg cgttctcgcc gagcgtcacg
    15421 ttcgcctcgg cgatgagcgc ggtgagcgac gcgaagccgt ttgcatcggc gctggtcgtg
    15481 ccgggcgcat agacgatcgc gccgttcgcg acgaggttat tgcggatgtt gagcacggtc
    15541 gccgccatct gcaccgagag catgatcgcc atgttcgtcg cggtgccgtt caggagccac
    15601 gagcgaacct gctgcttcgt cgtcggatcg aacgctgtcg acggccacgt gtagaggttc
    15661 tgctcgcgga gcgcctggag gtcgcctgca tcgatgagcg actggccgtt cttgttcgac
    15721 cagaagccga gcgtgtgacc gccacccgcg ccgacgcaaa cgttgccgaa gtagaggccg
    15781 ttcaccgtcg aattgtcgac cgcgacgacc gtgtactgct tggccgagag cgtcgctgtt
    15841 gcagcgcccg tcacgcttgt ctgatcgaca gtgttgcccg tctgatgcca cgcgctgttc
    15901 ggagcctggc gctccacgaa caagtacgtg tccgcgatca gtgtcagcgt gaattttccg
    15961 tcggcttcgg tcgcgacggt gccactcact tcatcgtggt aatcgatcgg ccagccaccg
    16021 atgccgggct cgcccgagtc ccacttgccg ttgatgttgg cgtcgtaata cttgccacca
    16081 ctcaccaccg ggaactgtgg ctccgatggg tcagcaccgt tgcgaacgcg gaatgcgtcg
    16141 tatttgcatt gcgacggtga ggtcgcgccc gagaggcaga tcgcgaggat atagacgccg
    16201 ccgttgttcg gcgtgtctgc gaacggtgcg aggccgagga tcgcgcgacc ctgcggcgac
    16261 gtgccctccg cgtgcgggcc gtcgtacgcg agcagatcga cttggtggtt cgccgtcgtg
    16321 aacgttcggt tcgcgaccga gtccgtgctg agcaggcctt cgcccgagaa gaacgtcgct
    16381 tgcgcacccg gcgcgacgac tgcgaagaag tagctgccgt cggcgagacc gctgacgttg
    16441 tgctgcgggc cacccgacat gaagacgtag tctttgccga tgtagctgtt gcagttgacg
    16501 ccgttgccgt tcttgcatcc gccaagagcg tcgtcgaagg tcgtgtaccc cgcgccggcg
    16561 acgatcttct gatcgccctg cgattccttg ccttcgcgcg cgtcaaagcc gacgcagcct
    16621 gcgaacgacg ccaccgtaac aacacccgca acgactgcct tcatgacacc gactcctgga
    16681 tatgaggttg tcggtataga gacgtttcct acttcacgtg aagtggcgac tcgactagcc
    16741 ggtggtcaac accgtgtaga tcttcgatcg gcggtgaaac aacactcgct gacagctacg
    16801 ccgaagccga aacagaatct tgttcgggct actgtcgcgc cggcgcatgc gtcgttcctc
    16861 gattcgcgag ctcccgttca agcacaatca gaagccgcgc ctcgggttcg agatcttcaa
    16921 gaagagcgag ctattcgcgc gccagccgga agctgacctc gttgctccga agcgactcga
    16981 cttccacatc atctgtgtcg gtctgcgcgg ccgcggcacg accattgtcg acttccagga
    17041 agtgccgctc ggtgcagggc acatgaccat cgttgcgcgc gggcgagttc acaacttcct
    17101 gccgaagcca ggcgtcgatg cgtggctgtt gctcgcgcga cctgaggtcg tgaccgggac
    17161 ctacgcgctg ctcgatccga tgtggatgcc gccggccgtc gagctgtctc gcgacgatcg
    17221 cgatgagctc ctagggctct gcgaccagct agcggcggag caaacccggc cgctcgacgc
    17281 gatccagccc cgcctcatgg aggcactgct gtccgcggtg ctgctgcgcg gcgagcggtt
    17341 ggtgcatgcc tcggcgtccc atcaaccggt ggcgcccgtg ctgcagcgat ttttcaccat
    17401 cctcgaggcc gactgtctcg gcacgcgcga ggtcgctcac tacgcgaaga gagctgggat
    17461 ctcggtgcgc cagctgtcgg atcttctcgt cgcgcacacc ggcaagtcga cgaagcgcgt
    17521 gattgacgag cgagttgtcc tcgaacagaa gcgtttgctc gctcacacgg atgtctcggt
    17581 caaggagctc gcggagcgca ccggcttcga cgagccgacg aacctcgtga agttcttccg
    17641 ccaccacacc ggaactacgc cgcaggcgtt ccgccgaaat ttaccatccg gccgccgatc
    17701 ctgacctcgc ggcgcgagcg ggttgcgact aggttgtggg catgtcgaaa acactcctcg
    17761 cctcggttat cgccctcgcc gctgtcgcag ctcctgccgc cgcggacacc aagctcaccg
    17821 ccacgccgta caccgcgacg ccgaccggct tcctcgtcaa ttcgacgctc gtctccggcg
    17881 agaaggaagc gatcctgatc gacggccagt tctcgctcgc cgatgcgcat cgcctcgtcg
    17941 cggtgatcat ggagagcaag aagacgctga cgaccgtcta cgtcacgcac tcgcatcccg
    18001 atcactactt cggcttgaac gtcatcaagc aggcgttccc gaaggtgaag atcgtcgcga
    18061 cgccggccgt ggtcaaagag atccaggcga gctggaaggg caaggtcaag cagtggggcc
    18121 cgatgtacgg cgcgctcgtt ccgacgcagc cggtcatccc gacgccgctg aagggcacga
    18181 ctctgacgct cgaaggccag acgctcgaga tcaagcaagg cgtcggcgac gcgcccgaca
    18241 cgacgtacgt gtggattccg tcgatcaaga cggtcgttgc cggtgacatc gtgttccgcg
    18301 gcgtgcatcc ctggacggcc gagggcaaag cggagggtcg caaggcgtgg atcaagacgc
    18361 tcgacgagat cagcgcgctg aaccccgagg tcgtgatcgc aggtcataaa gatccgaagc
    18421 tcgacgacaa gccggcgggc gtgaaacaga cgcgcgacta tctcgaggcg ttcgatgcag
    18481 ccgtcgcgtc gtcgaagagc gcggccgagg tgcaggagaa gatgaagaag aagtacggcg
    18541 accttcagct cgacgtcatt cttcagatcg gcgccggcgc gcagttccct cagcccgcag
    18601 cgacgaagtg atcagagtcg ccagcgcggg ctgaagtgat agccgctcgg gctgtagaga
    18661 tagttgtcct gcgtcatgcg cgcgatcatc ttttcgcgcg tgcccatgat ccagaggaac
    18721 ggcgcgagca ggattagctg atacggcccc atgatgccga tcaccacgaa cgcgatcgcg
    18781 atgacgcgcg ccacggtgac cgcggtgtcc gtggccttca cgtagtccat cttgcgcgtg
    18841 aggagcgcgc ggagaatgcg gccgccatcc atcggcagcg cggggatcaa gttgaacgcg
    18901 gcgaggatga agttcgccca accgagccac gcgaacagcc agacgtgcgt gatcgcaccg
    18961 aggccaacgc cgagcgcgcc gagcgcgagc gagacggcag ggccggccgc ggcaatgatg
    19021 acctcgtcgc gcgcgttgcg aggcagcgat tgcatcttcg cggcgccgcc gagaaagccg
    19081 aggtcgatgc ccgagacgtg gacgccgacg cgacgcgcag cgaccgcatg cccgagctcg
    19141 tggagcacga ccgaggcgaa tacgaggagc atgagcgcga cgccggtgac cccgccgaaa
    19201 ccgatcagca cgaggccgag caggaccacg aagctcggct tgatgtcgac gggaaacccc
    19261 agaaaggttc cgagtcggaa ggtcttcagc atgggcatat cgtgcgcacc cctacggtgt
    19321 tgtaaagcct acgccgacgg cgcgccaccg acggaaatat tcccggagga cgccgcctgt
    19381 tcgggcaggg caggcacgcg aaatgctgct cttttgatcg acctccttcg tagaaccgga
    19441 ggacgcaatg aagaggctag gtttccttgc agtgctcgca cttgcgacga cggcgccgat
    19501 cacgatccag ggcgcggccg ccgagccacg ccacatgagt caaaatccgc gcgttggcct
    19561 gctcgcagag ctgacgttcg atgcgggcaa gctgcggctt cccgatgcgt ccgggtcaca
    19621 gctcgggcgc gtcgccgcgt gggccgagga gaacttcgac ggcctggtcg tgatcgacgg
    19681 ccacgccgat cgccagggcg cgaaagcgga caacgttcgg ctcgcactgc agcgagcgcg
    19741 gctggttcgc gatcagctcg tcggccttgg tgtggatccg gatcagatca tcatcagcgc
    19801 gttcgctgcg gagacgcatc ggcagcgcgc acgcgtcgcg atctggggaa cgcgcaactc
    19861 acgcgagcaa gtgatcggtt cgcgccggaa agcgcacgcg atctacaacg gcaacgtctc
    19921 ggcgcggcag tcgccgacgc tcgcgggtcg tcgttagctc atcaaaatcg agaacagcat
    19981 gcccgcgtag ccggccttgg ccgcggcctt cgctgcgcgc tcgatcgctg cgtaggtgat
    20041 gctcgcgtgc gcggtgataa tgatgaccgc gtcggggcca ggctttggca gcgccgcagt
    20101 caatgccgca tcgagcttgc cgccgccggg cacggacatg ttgccgacga ggttgccgcc
    20161 gacggagacc tcgccggtct tgccaatgac gatgctcggt gcacggtcgg cgagctcgat
    20221 gcgaccgatg atcttgcctt cgttcgtcgt gcgtgacgcc ggcacagact tcggcggcgc
    20281 tggcaggttg aacgtaaccg cgccgattcc gaaatccgtg aagcccttcg cggccagcgt
    20341 gtccatcgcc gcgatcacat cagcgtagct cgtcgactcg ggcgcgatga tcgtcgcgct
    20401 gcacgcggga gccttggctg catccgcatc gagcgcgggt agcgtgacct tcacgacgca
    20461 gcgcggaggc ggccctgact tcttgttcga gcacgcgacg agcgcgacga cgacaaacgc
    20521 gagcgcagcg gacctcatgc gcagacgata cctcacgcgc gatggacgag ctggccaatg
    20581 cgtcgcgcga gctcgtctcg atcctcgggc ccgatccacg gcaccgacac gcgcgcgagg
    20641 tctgtgccgt gacggtccgc aaaccgcacg tagtcgccgc ggaccgtgac gccgaacttg
    20701 tccgtgacct cgacgatctt cgtgcccttg ccctccggca cgacgatctt gccgtcggca
    20761 ccgacgctca tcgtgaaccg cgcgcgatcg cgaaacagct tgcgggagta gatgacgtag
    20821 cgctcgccat cgtcggcaac gagcaccttg tcgagcggat gtccgctgcc gatctcgacg
    20881 ccgcggagca cctgcgagaa gccctcgacg tgcgcgcgta cacgttgcgc gagcgagtcg
    20941 agcttgccga gcgcgacgag gccggcaaat tcccacaagt ttggtggctc cacgccgaag
    21001 atgcgctgca tcggcagtgg ctcgcgcggt ttgcgcttct tctcgagcgg ctcttcggca
    21061 agctcgatcc agaggccaaa cgattcgcgc ggccacccgc gaacgacggc gatcggtccg
    21121 acgatcttgt gcgacgcgcg gcgcccctgc ttctgcttct tcgcctcgat gatcgtcgcg
    21181 acgccgtcgg cgatgtgcag cgtgcggctg ccgagcacca tatcgagacg gttgggcccg
    21241 agcgtcgctt tgagcacgcc gtcggcggtc ggcgcgtcga gcgcgtcacc ggcgtcgtcg
    21301 ccgcgatagg ccatgactta ttgttgcatg cagaggacgg acaactgcgc gtcgcacccg
    21361 ctcgtcgacc acgaccagaa ttgcgagccg aggatatgcg gtggcagccc gatcgggacg
    21421 ttcgtcatcg cgttgccgct cgtccagtcc atgcacgttt gcgcgctggt cacgttcggc
    21481 gtcgacgaaa cgccggtgta gaaccctcgt cggacgtagc tgccatcgga cgcctgatgg
    21541 acgaacgagc gcagcagccc gccggtcaag atcgcgcttg ggtccgacgc gagcgtgccg
    21601 tcgacgcgaa cccacggtcg ctgatcgacg acaaagcgcg acgcgatcga tgccgtcgac
    21661 gtgttcaacg ccgcgaggta cgtacctgga aaccctgccg ccgttgcttc cgcattgcac
    21721 agcgcatctg ccgcggcgac gttcgtcgag ggcgtccgct tcgcgaccgt gagaaacgca
    21781 agccgaccgg acgacacgat cggtgccacc tctgctcggt gaccgacctc gaagcagatg
    21841 aggtggcgcg gcatgtcaca tggcaatgga ttgccaaacg ccaaggtagc gttgcgcaac
    21901 acgttgccct ggcccgacgc ggcagccgag ctcgtcgtcc aatcgttgca ggtcgcggtc
    21961 gtatcgacct tcccatcctc cagcatgccg gtccatgccg attggttcga ggcgaaccgg
    22021 ctgccgtcag cgagcatgtc gaccgcattg aggacagcgt actgatcgag catcgcatcc
    22081 atcgtcgtgc ccacgggcgt tccgtcgacg agaacccaac cgcgcgagcc cgcgaacctc
    22141 gctcgaacat cgggaaccga cgacgacgtg agaaacgcga cgaacgtgcc ggggagccca
    22201 gccgcagcag cttcgcttcc gcacgtcgcg tcggcagcgg tcacgcctcc gaatcctccc
    22261 ggctggctcg cggtcgtagc gaacacgacg tttgctcggt ccggagcgtc cacgtcgtcg
    22321 gagcgcggat cgaaggcgag cctcccgcaa gccccgagaa ccaccgtggc aacggccagg
    22381 cgcagcacct cgtccagggt agccgaaccg acgacgcgca ggtataaccg gccgccatga
    22441 ccgcccagta cacgttcgtg atgaaggatc tccgcaagct cgttccaccg aagcgagaga
    22501 tcctcaaagg aatttggttg tccttctatc cgggcgcgaa gatcggcgtg ctgggcaaca
    22561 acggcgcggg caagagcacg ctgctcagga tcatggcggg cgtcgacaag gacttcctcg
    22621 gcgaagcgtg gcccgcaccc ggtctcaagg tcggctacct cgcgcaggag ccgcagctcg
    22681 atccgacgaa gacggtgaaa gaaaacgtcg acgaaggtgt cgccgacacg cgcgcacttc
    22741 tccagcgctt cgaagaagtc tcgatgaagc tcggcgagga catgtccgaa gacaaaatga
    22801 acaagctcct cgaggagcaa ggtcgtctgc aagatcagat cgaagcggtc aatgcgtggg
    22861 agctcgacca tcaggtcgac atggcgatgg atgcgctgcg ctgcccgccc ggcgatgcag
    22921 atgtcacgaa gatctccggt ggtgagaagc gccgcgtcgc gctgtgccgc ttgttgctct
    22981 cgaatcccga catgctgatt ctcgacgagc cgaccaacca cctcgacgcc gagtccgtcg
    23041 cgtggctcga gaagacgctc gagaccttca agggcaccgt cgtcgcggtc acccacgatc
    23101 gttacttcct cgacaatgtc gcgaagtgga tcctcgagct cgatcgcggt gagggccacc
    23161 cgttcgaggg caactactcc ggttggctcg agctcaaggc gaagcgcctc aaagacgaag
    23221 agcgccaagc cgacgcacgg cagaagatgc tcgcgcgcga gctcgagtgg gtgcgcagct
    23281 cgccgaaggc tcgccaagcg aagagcaaag cgcgcctcaa ccgctacgac gagatggtgg
    23341 ccgaagccca gaaggacgcg cgtgccgacg gtctcgagat cgtgatcgca ccgggaccgc
    23401 gtctcggtgg cgaagttgtc gtcttcgagg gcgtctccaa gtcgttcggc gatcgcctgc
    23461 tgatcgacaa cctctcgttc aagctgccgc gcggcggcat cgtcggcgtg atcggtccca
    23521 acggcgccgg caagacgacg ctgttccgca tgatcatggg cgtcgagaag cccgacaagg
    23581 gcacgatcaa gatcggcgag accgtcaagc tcggctacgt cgatcagtcg cgcgacgcac
    23641 tcgacgacac gaagacgctc tacgacgaga tcgccgaagg cgccgagacg ctcacgctcg
    23701 gcgagccgcc gaacgtgcgc acgctcaacg cgcgtgccta cgtctcgagc ttcaacttca
    23761 agggccagga ccagcagaag cgagtcggca ttctctccgg cggtgagcgc aaccgcgtcc
    23821 acctcgcgaa gatgatcaag aaggcgggca acgtcctgct gctcgacgag ccgtcgaacg
    23881 atctcgacgt cgacacgctg cgcgcgctcg aagacggcct cgagaagttc ccgggctgcg
    23941 ccgtcgtcat cagccacgat cgctggttcc tcgatcgcat cgccacgcac atcctcgcgt
    24001 tcgagggcga cagccacgtc gagttcttcg agggcaactt ccaggattac gaagcggacc
    24061 gcaagcgccg cctcggcgat gccgccgacc agccgcaccg gatcaagtac aagccgattc
    24121 gcgtctcgta acagctacgg ccgcggagac ggcggcttgt ggtcgtgatg atgcgcgtgg
    24181 tgcggatgat gtgcgtgcaa ccgcacttcg gcgacgagcg gacgatggtc actcgcgcgc
    24241 ttggccagct ccgagtcgca gcgcgagagc ttcatgaagt cgacgttgcc gcggacgaag
    24301 atcgcgtcga gcgggcgcac ggggccccat gcggggaacg tgagcgggcg gcgttcgatg
    24361 ccgcggaagc cggccggttt gagcagctcg ctgaggccac cgtagacgtc attgagatcg
    24421 ccgccgacga cgatcggcgt ttcggcgtgc agatttgcga atggatgact gtcgaggaac
    24481 gtgcgcagct ggatgcggcg ttcgaagcgc gcgagcccga ggtgcatgtt gtagacgtgc
    24541 acggtgcgat cgatgtcgtc gtgacggacg cgaatcacgc cgtgcagcgc gctgcggcgt
    24601 ttcttgaagc gccatgtgag gtcgatgttc gtgctctcga tcagcgggta gcgagagagg
    24661 atcgcgttgc cgtactggcc accaccgcgg acgtcgacgt tcgggtacca gcagcgatat
    24721 cgcatgccga ccatctcgcc gagcacttcg gtctgcttgt cgcctttgct gcgcgagacg
    24781 ccggcatcga cttcttggag cagcagcaca tcggcttcga gcttgcggag gacctcggcg
    24841 gtgcgttcgg gctcgtatcg gcgatcgaca ccgccgatgc acttgtggat gttgtagcta
    24901 acgatgcgca gcttcatgag tggggcttcc actgagcgac gacgagcgtc tcgtcatccc
    24961 cgcgcggctt gccgccgcga tgagcagcaa cgcccgcata gacgaggtga tgaacgcccg
    25021 gcggattgag actctggcgc tcgagacgac gcagcatcct ctgcagtctg cgatcgccga
    25081 acggctcacc caccgaatcc tgcgcctcga tcacaccatc ggtataccag acgaccagat
    25141 cgccggctcg catcggcttc tgcagaactt tcggaagtga ggagctcgat ccaccgagag
    25201 gattgcctct cccaacaagt gcctgcagct cgatctcgcc atctgcgcgc acgagatacg
    25261 gcgtcgtgtg tccgcatgag acgaacctga tctcgagtgc atcgggatcg aggatcgcgg
    25321 cgaagcaggt catgttcagc tggccaccgc ccacgcgccg cacagccgca tcgagcgctg
    25381 cgatgaggtc ggggagcgcg agctgcgcct gatatttgcg aacgcagacg tcgaccgcag
    25441 cggatgcgcc tgcagtgacc atcgcggacg gcacgccgtg accggtgaca tcgccgatcg
    25501 cgacgagcac gcgtttttcg ccgatcggat agacgcacca gaaatcgccg gcgcagcgcg
    25561 ctgccggtag ccacgagccg atcacggtga tgtcgccgtg cacgtgtggg cctggaccgg
    25621 ggaggagctg cgcttgcacc gtggccgcga gctcgacctc gcgagcgatt tgcgcgcgct
    25681 gtgctgcgcg ctgcgccatg cgtgcgtgca cgagtgcttc accgaggcgc tcggctgccg
    25741 actgcaagaa cgcgagctcg cggccgcgaa ctcggcgcgc gtttgcgggc agcgcgacga
    25801 tcgcgaggag ctcgtcgttg ttcgtgactg gaacgagcgc gatcgcgttg cgcttgtcga
    25861 agagtccgtt caagaggtcg tgtaggtcgg caggcacatc gccgagctcg tcgcgaaaca
    25921 gcggcgtgcg attctccgcg agccacgcgc cgaggagcgg atcgggcgca gcgtcgtcgg
    25981 cgagcttctg gccatcggca gtggcccagc cgtagtcttc cgcggccgcg agcagcacct
    26041 cgacgcgcgc accgacgccg agctcgacga catcgaccgc gagctgcgcg atcttgcctt
    26101 cggactcgag tgggcgagag cgcgtgacga gctgcgcgag gagccggtcg agcgtgctct
    26161 gactgccgcg cacgccccga ttgatcaggc tgacgatcgc gaacgtcgtg cgcacgcccg
    26221 caaacgagac cgcgagcacg gcggtggcga gccaccacgg aatcgccgac ccgagcatac
    26281 gcagcgaaac ccagccgagc agcagcgcga ccgcaaagtg gaagacgagc ctcggcgcag
    26341 tcgtgtcgat cgcgcgcaca cgcaatagat cctcgacgac gagcgcccgc acgacgagca
    26401 cgctgccgag gccggacagt agccagccga gcgggaagat gccgatgtcg taaacgaacg
    26461 cgacgtcggt gagcccgatg tacgtgatga agctcgccgc cagcatgagg cggagctgtc
    26521 gacgctcggc ccccgccttc atgttcttga cgtgaagaac gaactcgatg aacgcgggag
    26581 tcgcgttgag caccgtcgtc aggaggatca gccatgtgag cgatccggca ttc
//
LOCUS       DATLBZ010000412        36380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4205159, whole genome shotgun sequence.
ACCESSION   DATLBZ010000412 DATLBZ010000000
VERSION     DATLBZ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 36380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36380
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4205159"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VMZ53_20825"
     CDS             <1..1413
                     /locus_tag="VMZ53_20825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HUS30972.1"
                     /translation="SGLYNLDLDRGLDSTGQPLFTVPLSGGQFLDAGDLRARTDLALY
                     ARGVGVAVKARIDWLDNVAIGGSPDLAGGSPATSGGQRPTTVVVKRAWGEALTPAGTL
                     AVGRMGAHFGMGMAANGGDCEDCDHGDAADRLAFVTPLFGHLVAVAHDFASRGPFTPG
                     RDGGHPVELEASTAASGPTFGVLKVHSPATLARRADAGLTSVEYAGYVTHRAQENDVP
                     ATYLPTATPPSMYTSTDLVARGFSATGTGAWLRITSSRFRLEAEVAYAKARVAQPSLI
                     PGTEITVPVTSNQLGAAMQSDVIAGPTRLGFDAGYASGDSAPGFGAFPKAGQTSPMAG
                     AFDGPQADLPRDRTVDNFRFHPDYRVDQILFREIIGTVTDAIYVRPHVRATLMTVGSG
                     KLETVAAVIASWAVEPTSTPSGQRPLGVELDPELRYAHKDGFAINFAYGLLFPGAAFD
                     GTTLPAKPAQCFRLRMGFAF"
     gene            1413..2201
                     /locus_tag="VMZ53_20830"
     CDS             1413..2201
                     /locus_tag="VMZ53_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30973.1"
                     /translation="MRRAAFIVLAACGTNQTPQPDPVPTLPSCVPNRNGKITADELPI
                     AIGASVEYYVGTSRTITQAGTSWDFSAELADDDILELGPAPLSGQWYASSFTSGEFVV
                     DAGSGLDGIYHQDAQALWLDGIASHLETPPAGKTLIVYATPLPTLRFPVVEGQFFTTT
                     TTLAGATINGLPFQGTDELTVDVVSSGKLSVPYVSFSPVLRVRTQAVRKPSSAGVPST
                     SRRTTIFLFECFGEITRAESKPDETNPDFTGAAYLRRFALGQQE"
     gene            2219..2491
                     /locus_tag="VMZ53_20835"
     CDS             2219..2491
                     /locus_tag="VMZ53_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HUS30974.1"
                     /translation="MWKQGFAAWEQATAQYMEKVLTNPGVLGPAGAMLTAAMKTKAAT
                     DKALAQWWSTFGLPTRRDQERALHKLNQLESRLYDLEEQLHAAKKN"
     gene            2494..4110
                     /locus_tag="VMZ53_20840"
     CDS             2494..4110
                     /locus_tag="VMZ53_20840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HUS30975.1"
                     /translation="MDVSHLLQVAPAPRAVFDRLATHRHRVRFQVRKGSSWEPVTWGQ
                     FALQIRGVARWLVEHDLEAGERIAIYGHNSVAWASAALGAQTAGAVFVPIYPASTPEQ
                     VAYIVEHAEIKFVFVAGRDCQSRLERALAMVPVKPQVVDLEDPAFEAAGARDAAAPTL
                     VDARLGALDLDQPAQMLYTSGTSGNPKGVPLTHRNVGKNGADWLTCNAPLLTEGDRDL
                     LWLPMSHIFGFGELCAGNLLGWETYMATPADVLDLLPGVAPQVFMSVPAYWEKIARAI
                     VAGPGATDEGERGAEARKEALRRVTGGRLRFCLSGGAGLKVEIKQLLHSCGVLVIEGY
                     GLTETSPTLTLNRPDAFRFDTVGKPLPSVQLKLDTDGEILAKGENVFSGYFKDPGATA
                     AAFTEDGWFRTGDVGRWTDDGFLQIIDRKKDILVTAGGKNIPPANIEAKFVDDPVIER
                     VVVYGDSRPYLVAAVWLRPDVPRDEALRLASQRVEEINKELARHETIKRHFVAELPLT
                     VEEGTLTSSLKLRRKIVYDKLRERFEALYA"
     gene            4117..5145
                     /locus_tag="VMZ53_20845"
     CDS             4117..5145
                     /locus_tag="VMZ53_20845"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS30976.1"
                     /translation="MKAPLKKTPLDSIVALLKRAPKGPPPVGVTPHSVVWTENKWRLL
                     RFAPAQPRFATPIVMVPSLINRWYVLDLGPQRSLIEWLVAQGHEVFCIDWGTPGAEDR
                     YLTWDDIAGRYIGRAVRIAARYGRSGRTHVLGYCLGGTLAVSYVAAFPEYVASLLALA
                     APIDFEHAGIMSQWTRTPTFDVRAILDAFGNVPWPLMQASFNMLKPTLRAAKTVALLD
                     RAWDDEFLEGFLATEHWGHDNVSFPGACYARYIEELYRANRLMTGGFTVSGRPAELHN
                     IKCPVLALAFADDHIVPLPSASPLVDRVASGDKQLVVQRGGHVGAVVSRKAADRLWPV
                     MHDFWAAR"
     gene            complement(5157..5531)
                     /locus_tag="VMZ53_20850"
     CDS             complement(5157..5531)
                     /locus_tag="VMZ53_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30977.1"
                     /translation="MRLAIIASILLGLAACGGGDLSPKEGCNQSAAALCERLYACFTS
                     AELAAGGFPPNEAGCVTKYQTDYGCSAQTVENTCDGNEKYHGDQADDCVDQIHGLECS
                     QVRDANFNYETGLPACDKVCSI"
     gene            5571..6419
                     /locus_tag="VMZ53_20855"
     CDS             5571..6419
                     /locus_tag="VMZ53_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30978.1"
                     /translation="MTAANVLLSALLHALWNALLRLEKDKDRALVAAVSVATLLAIVV
                     GGTRWALGTLPFATTESVIWTVGAGVLEWIYFASLAKALDRGPLGAVYTISRGGAILV
                     VWPISIALFGEPLALTSAIGSAVVLGGLALAGIGAGSTKQTRIAALAWAIACAVSIAG
                     YHLAYKGAMAAGGSASAVFAVALAVATAINIVRLGTAGRRIARELVQQRPGRVILMGV
                     ICGGSFLILMEALRGGGAGYVLTLRNTSVLFATLFAALIGERPRRPEVLGSLLVAIGA
                     VLMAWH"
     gene            complement(6416..6802)
                     /locus_tag="VMZ53_20860"
     CDS             complement(6416..6802)
                     /locus_tag="VMZ53_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30979.1"
                     /translation="MRRYLLVILLGGCAVDGQVDVGAQEDAVTCTRVEGKDLGERLTV
                     QVRGIDTVRDVTFTDWQPKADGAGYVGFTLDVATSYIVKADFNFFYDAGTAWQNPFGE
                     AGRLAIPIDYVDVCEAFAEDEPALRF"
     gene            6835..7737
                     /locus_tag="VMZ53_20865"
     CDS             6835..7737
                     /locus_tag="VMZ53_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30980.1"
                     /translation="MLRRVWLALAVTGCKLHFDDSHDAATTERPCPDDFIFVTGNPAL
                     GTSDFCAMKFEAKARDLGTQQIVANGCNATCDPDWGSTGYGAASASEGAPFRSVNALD
                     AQRMCRELGTGYDLMANREWMTIARQAEANPANWQSGTPGTGRIAQGNTNGSTPGQLA
                     CTDESDPYNGTGDDAMTAWDQRRIEVLANGEVIWDFAGNVQEWIDFTLAGPYDPPPSC
                     SSSELPTVSCAGLSRDDFQSTAGTLDSTNGVGKLIGGSGNALRRGGQTGDFAGDFAGI
                     YALNMNRFTNDTFPATGFRCVLRL"
     gene            7750..8370
                     /locus_tag="VMZ53_20870"
     CDS             7750..8370
                     /locus_tag="VMZ53_20870"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HUS30981.1"
                     /translation="MRLEHVRRAVAFGAHPDDVEVGAGGLVAKLTNGGAHVAIVVTSI
                     PNQYAVRRAEATASAAKLGAALVLPPGDETGSRIEDVPMSALVARFERELASVEPDLV
                     IVHGSHDSHHDHIAVHRAVLAALRRTRCDILAFATRLPAGAAPPPPTCVVDISSTIEQ
                     KLAAVAEHKSQFSADFPEKRRDIHRALGITHGMQYAEVYEVLRIEL"
     gene            complement(8371..9219)
                     /locus_tag="VMZ53_20875"
     CDS             complement(8371..9219)
                     /locus_tag="VMZ53_20875"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS30982.1"
                     /translation="MAAAGTLEPVAVTVPPRSWRSDIRAIRIVWHRDVIRFWRDKPRM
                     LASILQPVLYLFVFGTGMGAGMRPTGGLDLRVFLFPGVMAMSTLFTCFFSAGSIVWDR
                     EFGFMREMLVAPVRRYSIVVGKALGGATAGMFQGVVVLAMGPLVGVHFTPLLVLTLLA
                     ELAVLSFAITSIGVLVAVRIKSFQAFMALVQIIIMPMFFLSGALFPMKGLPTWLHIAT
                     LLDPLTYAVDPMRREVLGQFVDKSLVPGVTWSGWQVPTVLELAIVMAVGLLALAAAAV
                     EFGKTE"
     gene            complement(9222..10208)
                     /locus_tag="VMZ53_20880"
     CDS             complement(9222..10208)
                     /locus_tag="VMZ53_20880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS30983.1"
                     /translation="MTAPAVSVHGLQKRYGDIEAVRGIDLEVAAGEVFGFLGPNGAGK
                     STTIKILCTLAKPTAGEAKVAGHDVMSERDEVRRHIGLVFQETTLDDYLTAEQNLRFH
                     AELYGIPSGSVAPRMQQVMEMVGLWERRTHRVQTFSGGMKRRLEIARGLMHSPRVLFL
                     DEPTVGLDPQTRASIWKYIRELKQRETITIFLTTHYMDEAEYCDRIAIMDNGKIVALG
                     TPEALKAKVGKDRVQIATADDAAAISALKDKFQIDAAKREGMVMFNVEHGEQFLPRLF
                     AELGIPIRSVSVARPTLDDVFLAYTGSTIRDAEASAFEKSGMAKFARAARGR"
     gene            complement(10218..10571)
                     /locus_tag="VMZ53_20885"
     CDS             complement(10218..10571)
                     /locus_tag="VMZ53_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30984.1"
                     /translation="MKLAVIALVVGLTGIAHADAPQQKRPLKQLLLQKFDRNHDGKLG
                     PRERKHAAKVLHKLANRLAKADDRAQRRSKFIRHYDANNDGNVGPGEMPPALADELRP
                     LDRDGDGWLQGDELP"
     gene            complement(10601..13516)
                     /locus_tag="VMZ53_20890"
     CDS             complement(10601..13516)
                     /locus_tag="VMZ53_20890"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HUS30985.1"
                     /translation="MVVRARRLVVAGCLLASLEAHAVVPTQGPQISADALTAMPAAGT
                     AKPLRVERKLEWAQAPSAAWSTFAQRHTGWQAGWDRATGVPSRMWGPGIAAPGSVADP
                     ATAERIARQVLADHLALFAPGSQPSDFELVSNHFDGSMRTLGFIQRSGGIHVIGGQIS
                     FRFKADRLFLIGSEALPNVSVATTKAKLAPRDLQRRATEHLRTAVALPNAPVTQPGDV
                     VILPLVADDAVLGYRLARPVTIDGGGAGRYVGYADASTGDILALRQLNAYATGVVNYH
                     VVDRYPERGRLDLPAKRAHVTVNGAAQTTSATGVVSWSDGEASVATGITGDLAAVVNK
                     ATGNAEATALYALQPGSQFAWDASGSESEDAQVNAYVATMIAKDYVRANIDANMRTLD
                     EVIPVNVNIANSCNAFFDGKAINFYQSNMQCQNTALIQDVVYHEYGHRVHTAEIIDGV
                     GDFDGAMSEGAADFLAATITGDHGMGRGFFRTSEALRDLDPDDKEWLWPQDIGEIHHT
                     GMIFGGAFWDLRKGLIADLGEAPGIQLTNRLYVAALRRAVSIPTALVEVLAEDDDDGD
                     LSNGTPHECTIRAAFGRHGLRTASGRVVAPGQLTIPALAIGVQIQVSGLSDRCNLDQV
                     QSAFIDWVPSFGEPHAGTAEATPAGPNAFFAQLPLAQQESIYYKARVKFADGSSMTLA
                     DNLADPYYQLYQGRTVKLYCTDFESTDPFAEGWTTGTDKEGASPFEWGTPTTGPTDPH
                     AAFSGSKIVAQKLDGDYNAEQDAWLKTPVIDIGQYSDVRLHYRRWLAVEDGHFDKAQI
                     MANGKKAWQNYDSDLGDSSAIHHVDREWRFHDVPLSGYISGHTAQVTFDLASDAGLEL
                     GGWHIDDVCIVANPYAICGDGVRTRTEQCDNGGMNADVADTCHTDCHLPTCGDSIIDS
                     DEECDDGALTDTCSSMCKIIEPVDTGCCSASGGGGSITLAGAVGMLLLRRRRRRTRV"
     gene            complement(13571..14578)
                     /locus_tag="VMZ53_20895"
     CDS             complement(13571..14578)
                     /locus_tag="VMZ53_20895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HUS30986.1"
                     /translation="MSLEQVAEAWGWRADQVAPLAGGLINKTYVISEGDARIAVLQQL
                     HPIFGAAVNLDIEAVTTHLASRGLPTPRLIRTLAGNAWVDHVGHVWRALTYVEGETVH
                     RVPSVEWATAGGALVGRFHRAIADLQYDYKFTRSGVHDTSRHLSKLQGALEAGGDVEA
                     MELGREVLRAARGLPVLPDAPKRHVHGDLKISNLIFQREPLAGVCLVDLDTFAKGTMA
                     FELGDAMRSWCNPHGEDAGSVRFDLSIFHAAIAGFRAEANDLLTTEERVSIVTGLETV
                     CIELAARFAVDVFEDRYFGWDAERFPSRRAHNLVRARGQLQLGLQVRSARGEALAAVS
                     G"
     gene            complement(14580..15854)
                     /locus_tag="VMZ53_20900"
     CDS             complement(14580..15854)
                     /locus_tag="VMZ53_20900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HUS30987.1"
                     /translation="MRAVILVALLGTVGCKKEAQQDNSKVTSGPGVAAKAAPQVDAPF
                     DLKNPPGDAVKTASGLIYKPIKPNPTGPLPKRNDTVMVKYTGWRQASGETFFSNINSE
                     SPMPLNLAKAAKGFTEGMQLVHKGEKAMFWVPAAIGYEDGKPPPANPETLVYEVEVVD
                     IKEAPAVPADVKGPPPDALSTKKSGTKYVVVRQGTGKDAAHIADEVTFHYTVWDAEGK
                     MIETTEMKKQPARSTPYKQAVAFEEILEQMVPGERVRAWVDAAALKEQVKTVVTGPVV
                     IEVEVLQIDKAKGDPPPSPPDVAKPPGDARKTAKGVFYKVLKAGKGGDKPKPTDTVRV
                     HYTGWQTDGRMFDSSVIRGEPSEFSLQGVIAGWTDGLQQMSVGDRFRFWIPDDLAYKG
                     QQGRPQGMLVFDIELLEIKPAAPHADMPPGHH"
     gene            complement(15851..17143)
                     /gene="pepQ"
                     /locus_tag="VMZ53_20905"
     CDS             complement(15851..17143)
                     /gene="pepQ"
                     /locus_tag="VMZ53_20905"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: protein motif:HMM:NF010133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HUS30988.1"
                     /translation="MSFDNYPDHVANLQRATEKILADQGYEALILCSGAPQSRNRFDD
                     QSWPLMPTPAFTHWCPLVEADSYVVVRQGRKPTLVRTVVDDFWETVPPPESEHFWEQF
                     DVVTVGPGHAGDVLPGGKVAVVTRDPGQAPPGEVNPATLIAALDGTRTRKTAYEIECL
                     AIASRRAVRGHKATEKKFQTTDASELELHLTYLDASEQDDPTTPYKNIVALGAHSAVL
                     HYVAYERKKVSGDTSLLVDAGARCNGYGSDITRSYVRGTSGPAKRFGELIARMDQLQQ
                     DVLKRIKPGLQYEELHDESHRLLGDMLKDVGIGKASGQELVAKGITRALFPHGLGHSL
                     GVTVHDVGMKLRPPRPENKFLRNTSTIEVGHVFTIEPGIYFIDALVGPLREDAERGPL
                     IDWKLVDELRPFGGIRIEDNVVVEADGIRNLTREAYKA"
     gene            complement(17154..18038)
                     /locus_tag="VMZ53_20910"
     CDS             complement(17154..18038)
                     /locus_tag="VMZ53_20910"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUS30989.1"
                     /translation="MRHLALALVLLVASPAIARAEGFTVGLFAPTAPFPSTSARVELA
                     SRLAEHLGNSINKTGSGKVFARGADFAAAAKKGEVSVAVVDAAYLAVAGGNYTLLASA
                     TRNGESVHGWQIVARGADKLAGLKGKRVLVPAIGGRETELVLNVLLGGEVAKDYFAKI
                     ETAPDTVSALAALGLGKVDAVVVPRGTDLPAGAAVVLELPALAGPVLVAYGTLSAADK
                     TSITNAALSFKGDATVSGFKAADPDAIKSVARRFTVPAKRGPLALPAIRLLVGDLVEG
                     RTFAIERTPVTAFATKPR"
     gene            complement(18035..20449)
                     /locus_tag="VMZ53_20915"
     CDS             complement(18035..20449)
                     /locus_tag="VMZ53_20915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS30990.1"
                     /translation="MSRSAIAISLVCALAATAAAQRANQGEDESVAFVEEGRAALRRG
                     ELDDAARSLDQALSLNPRRVEAYVLRSAVYAARKQYTAGIALMRKAQLLAPQDSDVLT
                     ALGSQLVLSGDVNAGVPYLEQVASKESARYDAQLLLGRYYHDASRWPESITALELYFT
                     HRPQQLAKEDPRYRVELADSYLRSGQPSKALPLFEHAAKANPKADLRARIGIAWSTAA
                     IDCKKARGMLKELEPIAEQGYAEIWLVDGQCALALGDPNGALALGRRYLDRVAAEKAK
                     GSAAGHALVGEAQAARGNLPDARKELELAASLEPQRRRWTVRLAVVLRRANAAADAQA
                     VLEKLGPPAPASADPDWWSELGETLLAQNAADKVVARLTPVVAELPTHAPTRIVLATA
                     LLATNKPEDAVKLLDEAEAIQSTPRSRKLLSEALTTVAADKLAANDAAGAEPLLMRAD
                     GLDGNPLVWRNLGIARLALDKKTDAVAILDKAAKADASPVVTMLAARAHAVAGDIAGA
                     RPLYERALAGDKDNLEIAIDWAASEVAGGDPALAVAALEKTATAAAKGPLAARHKAAL
                     ASARHAAGLAQLRAGNGSKAVELLRAAATPGEATLARKCDLAVAAVVAGDNAAALAAL
                     RAVQGQTCPFPPPADTQAVPILIAFTDGLNAKKAPNALTKLTSLGGKSTGIAAQLLGT
                     SIRVVALNAAQDAYRGGQLAPARKYLATAKTATARVGTDEVAHNLAVLDLVDGKVDVA
                     IAQFEKLTTRVPEAWVNLGIAYEKKGDHARALDAWRKARKMNVRFAPLTEWIEAKERV
                     YGGEAP"
     gene            complement(20424..22499)
                     /locus_tag="VMZ53_20920"
     CDS             complement(20424..22499)
                     /locus_tag="VMZ53_20920"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS30991.1"
                     /translation="MKRALALALLLATRAAHAQGAYVDVQDLPPLPPASEEESSSATL
                     LAASQVEEDVVVGAAKREQSLGNVASAVTVVSGDRLRRFGYRTVGEAVAGVAGVYISD
                     NRISYSVGIRGMQVPGDFNTRILVLVDGASVNEGWGSFAGLSFDNFVSIDDVARIEVI
                     RGPVSSLYGSNAFFGIVNIVTRSAAETPRAWAKASINSIWGVTTSAGFATGDVHQQLR
                     GTFQQMNRLGDTSSVPDVGSGLEGDASHQYMLSLVGSYQGSFAQVRAVQYRRDSPFAP
                     YNGDPADPDPYNQYDKQLLVEGGHTRELSKRFTLAGRGYANLYRFTDHINQINGIPFD
                     DLGEAKTFGAEVRGRYELVMPNKLGVTAGTEANYNVTRSVAQQLDGTSEPAEVPLDFA
                     VVGVYTELDGQPTDWLGYTAGLRFDQNTKIDRSLSPRAALFLSTHEKYGLKLLYAQGF
                     RNPSAFEGFFTDQTSFAANPDIGSERIYSGEVVAWAKPVAGLSTRLSGFYWDARDVVE
                     QLPFTDPVTGMDQLQFQNADRFVTRGVEVEASYRNAAGWYSFGGLSYQQVGAESETAP
                     LTFGEVPNAPAIIGSVGVSTPKLGNYVHLSSEAIFVGERPTRPDVDTGDALDPAPAWV
                     GWNGSVYVPDLRGFDLTAGVRNIIGKRQRIVAPGDYDRYDETTMSTTTIPLVPGEGRE
                     IYVKVGYRY"
     gene            complement(22496..23722)
                     /locus_tag="VMZ53_20925"
     CDS             complement(22496..23722)
                     /locus_tag="VMZ53_20925"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HUS30992.1"
                     /translation="MHAHVGGGADWVYGAPITLGNNAVGALRIGVTTAGLEAELQKSI
                     ATADEKSKASQQRLLMIALLVLGIGVVFAALQGVSLARPIKQLTVQAKKIASGDLKSR
                     VPATRRDELGVLARTFNFMADEIVDLLAEQRQKVSLEKEMELARQVQQAMLPPMNLDQ
                     HGYLKVIGYCQPASQCGGDWWMYRKLSGGRMLLVLGDATGHGIHSAMIAATARGAVEA
                     LAAVDERLLTPEQVLRAIDHAIRMVGDHNVLMTAFAAVFDSQTGALHYANAGQNFPYV
                     ITLGNTRVLERASIIAASGNPLGDRNIAVEIRRGSLQMRAGDLFVCFTDGVVERANPA
                     GKLFGDRRLRSALTGQQLQDDRGLIMLRDTVLAALERHADGKQADDDITFVLCQFDPP
                     ARSSTVSGTGIGRGAA"
     gene            23995..24159
                     /locus_tag="VMZ53_20930"
     CDS             23995..24159
                     /locus_tag="VMZ53_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS30993.1"
                     /translation="MIDFSPAKRRALICVRVRSSMVRWLNHVAAPTATATTTDVVRIN
                     FAEIGIPARP"
     gene            complement(24188..24739)
                     /locus_tag="VMZ53_20935"
     CDS             complement(24188..24739)
                     /locus_tag="VMZ53_20935"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HUS30994.1"
                     /translation="MTRCPRCLMQLRVCLCAEVPVIATRTRVVIVRHHLERARTSNSG
                     RLANLALTNSEIVEHGGSAGPAKLEGLDGAWLLFPEGEVTTVCPTPPPKQLVVLDATW
                     SQARRMYRKIDSLRGLPMLRLPVEPMAAERLRASPGPGRVSTIEAIARALRLLEGEEP
                     AAGLERLFAVAVERVQSMGRRIG"
     gene            complement(24741..28292)
                     /locus_tag="VMZ53_20940"
     CDS             complement(24741..28292)
                     /locus_tag="VMZ53_20940"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HUS30995.1"
                     /translation="MKFLARFSPMILALALVSSSQLSCSVNDYCLNCENGDGGHGSGD
                     GGMDGNGSNDGGVDAGCIPSGGPELCNDKDDDCNGLVDDNVSDEGGPCDNVAGACAGG
                     IEICDDGELKCNKQPQAEICDGVDNNCSGPIGTGNGVDEGDPGPPPAKNAPEMDPLVG
                     TPQCGNGLDDDSDGVVDDGCPGTGGKCGTDQGECIAGQFHCDPTTGTVRCFGFIDHRN
                     DAEACNAKDDDCDGVVDDNVPSGGNCGPTTNVGACEFGTINCVGGQPTCQGAVFPKFE
                     TCNNMDDDCNGSTDEIFNKTTDVRNCGACGNICPTRANANATCTPQGNPAVGTCGFAC
                     KPGYKDINGLVADGCEFGPCFPTGTVEECDGQDNNCNGMTDEGLTPPPLCLKGGECGG
                     GYTDPNGSNPLGPAPSAVCSGSGGWTCTYPGTVQFPETLCDTKDNNCDGNVDENQPNK
                     GQSCHEDRENPSTQCGADTVDNDGDGRVNDGCPRGGTGGASGPETLGNCANATDDDGD
                     GLVNDGCPAIAQLGACEGSGTYQCDPLNLNGAAYCAITTPGSAPAANESCDDTDNDCD
                     GKTDEGGANGTLVGQDWVDLGNGRQMMKYEASRPDSTSSSAGSKNTLVCSKSGVQPWT
                     NVKYQDALAACRAVGAQLCSESQWHHACSVVGTAAGYPTYPISFSGTGLYVEAEDYSS
                     TGVGAVSGSAEASCTDSESAGSCGNNTADEDSDGTVNDGCPVVGTAETFTQCQNGIDD
                     DGDNAVNDGCPAVGVDNDNDGFINDGCAFVGTAPETQCHDRVDNDGDGTVNDGCPAFG
                     PSPEASCNEAPAAGGVDDDGDGTINDGCPANGAAEVNCNDGVDDDGDTRINDGCPAAG
                     PAWVPDYTTVGTATNQQFSGISAMEATPNIGAVVSAANAPALAARLDYAVNFTTAGTN
                     NYHVWVRMFTSSTANDAIHVGISLLPPPQVPTNTITVGTTNAWTWVDAGTFSVPQGVR
                     YVSIYMNEDGAKVDRIYLVSGATNPGTGETLATKGNKWAYASAPNTYAPNTCNGHDYR
                     QVAVTSYARANTGIVTVTSASHPFVAGDLVQIQGASNSTLDGTYTVLTANATTFTYQQ
                     NSTSAIASANGGTATEARDPTLTTGSLTSCVANTGTGVFDMSGNVREWTLAHSPGENP
                     IRGGASNGTADGISCPLNFTLADDTFFFPNIGFRCCR"
     gene            complement(28289..32161)
                     /locus_tag="VMZ53_20945"
     CDS             complement(28289..32161)
                     /locus_tag="VMZ53_20945"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HUS30996.1"
                     /translation="MDDVTGSGPTSCGKVDNKINHAVCAINNIVNSYGDMVFSLARFR
                     ETTSGTYTNSCGPGGQCTTRGPQCGDCNYILGIGAPCTANSGCDSANGTTGNGGSCIL
                     TGADGNCTDNIDNDGDGYVNEGCATVSGTDENRNFTVNGITYNGCLDNIDNDGDLRVN
                     DGCPAFGRCTEGSGGTTVLTTRGCTTADDGIEILTPLVDGNNSSAALVTNGTCNTCSI
                     TGTTDPEIWGVGGTSTPLAAALKGAKRYWQGFQASNGTTTIWQQTLAGFDPINRDPQN
                     AVFLQAPSTVPTTCNSDRATCNPSVACTGANCCCDTCNPNPTTCNSSGTCVGANCCCA
                     TQCRPYVTILLTDGDETCANFTNSTVPAAASLLSTAPRVDGVNVNTFARSANVATVTT
                     TGAHPFAVGNVIVVSGSTDASFNGTFTITSVTSATTFTFASTGSNTGATSGTPATVRH
                     AASDFAYRIETKPIGFGKAPGDAEIEAIAHAGGAADLPGVNEGYYAANEQDLSLAISQ
                     ILADSVRSESCNGRDDDCDVRVDEDFPNKNQSCQATWPANDPISPGGVAVGLCARNGT
                     SICRPDGTGTQCNTVPNPRGTEGPGCNGLDEDCDGRVDEGLSCQQCVPTGEVCDGNDN
                     DCDTVADQTCRCSNNNSVKCDGTNTACGTGTCVCTGISQGCSVTNSFGTCPGTRVCTG
                     GSLGTCTGQVPAAEICNGADEDCDGVCDGFTLGCSEVVSNCVPSVGNSCPPADNPGEA
                     SHGPILHSESGLRCQNNTDDDGDGVVNDGCPTFPVAGGVAESACSGAADDDTDGRIND
                     GCPAVATALIPQNVCHAGSKTCAVPASCPAGSNAFGICSGEVKPAASDACNALDDDCD
                     NAIDEDFTPADCSTNCGVGQTQCVGGVITCNSTPATNDNTCNNVDDDCDGKFDENWVC
                     NPAANCTGPDCCVCGTGTTCEKNKCVNGVRTCVVDPPINTEVCDCNDNNCNGQIDEGQ
                     LCPSGSTCTGCQCAFGCDPNTEFACPSGKRCIQPSNTTACTIDGDCPTDEFCNQTNHY
                     CASDNFFCVNDPCYGVSCPAVNGNPQKCIPNPDPTHANEHQCVNLCDGVTCSSTEICL
                     DSDGICHPNNCLTFPNMCNAQQACVNGTCVTNPCQGVTCASNQYCLGGTCVQSCADLE
                     CADGQRCRQGMCETDPCGKPCPFGQACNDNTGKCINDPCSVQNCGTNSGQWCNPNSGQ
                     CEDDPCAINDIKCPNADEVCRGGTCLDPDSLRPDAAGEAHVTVGGGGGCDTTGGGSTG
                     VLLALALMVARRRRARVSSAARGEGGAQ"
     gene            complement(32369..33937)
                     /locus_tag="VMZ53_20950"
     CDS             complement(32369..33937)
                     /locus_tag="VMZ53_20950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS30997.1"
                     /translation="MNVIAVAARSLADRAVEAARQLTRNGEGIDEQQVVVERVAYAAT
                     EARVISELADTPAELADAARVAAGELANSVRNRLEPVAGLLGLGELRFDDAARAAINE
                     AISPSAVEAIGEAAIAAAGRLAWPLDETLTEVRANVRAFADREVAPNAERIHHHDELV
                     PERFIDEMAKLGYFGLSIPEKYGGHELGNLAMILTTEELSRASLAAAGSLITRPEILA
                     KALLAGGTEAQKQHWLPKLAAGEIMAAISVTEPNVGSDVASVACRAEKTTGGWHITGA
                     KAWCTFAGRADVIALLARTDPDMSKGSRGLSLFIVDKPRFPGHDFEAKQDGGGTLVGK
                     ADRTPGYRGMHSFTLAFDRWFVPDAQLVGGEEGAGRGFALQMAGFAAGRLQTGGRACG
                     VTQAAIEQTARYVAERRQFGRPIGEYGLTRYHLGRMMARLTAARAITYAAARAMDGDE
                     RQAAPVAAMAKLFSCDVAVEVTQLGQLLHGGWGYAEEYPISRYVADAQVLPIFEGVKP
                     ILALKVVGRSLLGR"
     gene            34012..34410
                     /locus_tag="VMZ53_20955"
     CDS             34012..34410
                     /locus_tag="VMZ53_20955"
                     /inference="COORDINATES: protein motif:HMM:NF022703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HUS30998.1"
                     /translation="MKSFLCLLLALTPAVAHADKNYRSGKGATWDCKKDPKVNISHGK
                     GVYTFKGACTQINLTGGKSKLTIESVDELNISSADNTVTIGTLGALNLVGANNKITYK
                     AAKEGDTAQVSSVGTGNVVEKIGGATAKRQ"
     gene            complement(34441..35268)
                     /locus_tag="VMZ53_20960"
     CDS             complement(34441..35268)
                     /locus_tag="VMZ53_20960"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HUS30999.1"
                     /translation="MNKAEIAKALGSAIKSIDSVSGGDINAAHRVVLEDGRRLFVKSH
                     DSPPPGMFASEAFGLEWLRDGPIRVPKVLAQGESWLALEWLDMRGRPDSIAFGRALAG
                     LHRMGLPTFGIDELQPSYLATLQQDTKPEPDWPTFYVERRLRPLCRRAKLGLDRELDQ
                     LRARVDVFGPPEKPARLHGDLWWGNVSAIPTAREAVPVIFDPAVYGGHREIDLAMLAL
                     FGGLPDALVEAYDEAWPLADGWRERIALHQLYPLAAHACLFGGGYGEQVKSALAGYL"
     gene            complement(35288..35758)
                     /locus_tag="VMZ53_20965"
     CDS             complement(35288..35758)
                     /locus_tag="VMZ53_20965"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HUS31000.1"
                     /translation="MRICFVCLGNICRSPTAEGVFRKLVEEAGIDRKLTIDSAGTGAW
                     HVGELPDSRSRAAAQRRGYELTHRARKLVAADFDRFDLILAMDRDNYDTIQRLARGRT
                     QLPSIRMLRAFDPSAPVDAEVPDPYSGEDDGFEEVLDICERACRGLLDHVRERL"
     gene            complement(35771..>36380)
                     /locus_tag="VMZ53_20970"
     CDS             complement(35771..>36380)
                     /locus_tag="VMZ53_20970"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HUS31001.1"
                     /translation="QRDATLEAWGQVPCSHGRPPSHMDSAQTLLLNQGFEPIKVISWQ
                     RAISLLFLGKVEVLEEYDRDVHSVTLIIKVPAVVRLLRSFRRHARPVKFSRVNIYARD
                     NYKCQYCARKASIQDLTYDHVVPRSQGGMTEWTNIVTCCYLCNRKKGGRTPQEAGMKL
                     LAQPAQPNWVPAVAIRISLRSVPEAWRDYLYWTGELDSGPGS"
BASE COUNT         5877 a        12148 c        11885 g         6470 t
ORIGIN      
        1 agcgggctgt acaacctcga tctcgatcgc ggcctcgact cgacgggcca accgctgttc
       61 accgtgccgc tgtccggcgg ccagttcctc gacgcgggcg acctgcgcgc gcgcacggat
      121 ctcgcgctgt atgcgcgcgg cgtcggtgtc gcggtgaagg cgcgcatcga ttggctcgac
      181 aacgtcgcga tcggcggcag tcccgatctc gccggcggct cccccgcgac gagcggtggt
      241 cagcgtccga cgacggtcgt cgtcaagcgc gcgtggggcg aagcgctcac gcctgcgggc
      301 acgcttgcgg tcggccgcat gggcgcgcac ttcgggatgg gcatggccgc gaacggcggc
      361 gattgcgaag actgcgatca cggcgatgcc gcggatcggc tcgcgttcgt cactccgctg
      421 tttggtcacc tcgttgccgt cgcgcacgac ttcgcgtcgc ggggcccatt cactccgggt
      481 cgcgatggcg gacatcccgt cgagctcgaa gcatcgaccg cagcaagcgg cccgacattc
      541 ggcgtcctca aggtccactc gcctgccacg ctcgcgcgcc gcgccgatgc aggtctcacc
      601 agcgtcgagt acgcgggcta cgtcacgcat cgcgcgcaag agaacgatgt gcccgcgacg
      661 tatctgccga ccgcgacgcc gccgtcgatg tacacgagca cggacctcgt cgcgcgcggg
      721 ttctcggcga ccggcaccgg cgcctggctt cgcattacgt cgtcacggtt tcgcctcgag
      781 gcggaggtcg cgtacgcaaa ggctcgcgtc gcgcagccat cgctcattcc cggaaccgag
      841 atcaccgtgc ccgtgacctc gaaccagctc ggcgccgcga tgcagtcgga cgtgatcgcc
      901 ggcccgacgc gccttggctt cgacgcgggc tacgcgagcg gagacagcgc gcctggcttc
      961 ggcgcgtttc ccaaggcagg ccagacctcg ccgatggcgg gcgccttcga cggaccacaa
     1021 gcggacctgc cgcgcgatcg caccgtcgac aactttcgat ttcatcccga ctatcgcgtc
     1081 gaccagatcc tgtttcgcga aatcatcggc accgtcaccg acgcgatcta cgtgcgcccg
     1141 cacgtgcgcg cgacgctcat gacggtcggc agcggtaagc tcgagacggt cgctgcggtg
     1201 atcgcatcgt gggcggtcga gccgacgtcg acgccgagcg gccagcgtcc acttggcgtc
     1261 gagctcgatc ccgagctgcg ctacgcgcac aaagacggct tcgcgatcaa cttcgcatac
     1321 gggctcctct ttcccggcgc ggcattcgac gggaccacgc tgcccgcgaa acccgcacag
     1381 tgcttccgcc ttcgcatggg gttcgcattc tgatgcgccg cgcagcattc atcgtgctcg
     1441 ccgcgtgcgg cacgaaccag acgccgcagc cggatccggt gccgacgctg ccgtcgtgcg
     1501 tgcctaaccg caatggcaag atcaccgcgg acgagctgcc gatcgcgatc ggggcaagcg
     1561 tcgagtacta cgtcggtacg agccgcacga tcacgcaagc cggcacgagt tgggatttct
     1621 cggcggagct cgcggacgat gacatcctcg agctcggacc tgcgccgctc tccggccagt
     1681 ggtacgcgag ctcgttcacg tcaggcgagt ttgtcgtcga cgcgggcagc ggcctcgacg
     1741 gcatctatca ccaagacgcg caggcactct ggctcgacgg catcgcgtcg cacctcgaga
     1801 cgccacccgc cggcaagacg ctgatcgtct atgcgacgcc gctgccgacg ctgcgcttcc
     1861 cggtcgtcga gggccaattc ttcacgacga ccacgacgct cgcgggcgcg acgatcaacg
     1921 gcttgccgtt tcaaggcacc gacgagctca ccgtcgacgt cgtcagctcg ggcaagctca
     1981 gcgtcccgta cgtcagcttc tcgcccgtgt tgcgcgttcg cacgcaagcg gttcgcaagc
     2041 cgagctctgc cggcgtgccg agcacgagcc gtcgtacgac gatctttctc ttcgaatgct
     2101 tcggcgagat cacacgcgcc gagagcaaac ctgacgagac gaacccggac tttaccggcg
     2161 ctgcgtatct caggcgtttt gcactaggcc aacaggagta gacacgatga tctgggacat
     2221 gtggaaacaa ggcttcgccg cgtgggaaca agcaaccgcg cagtacatgg agaaggtgct
     2281 gacgaatccg ggcgtgctcg gaccggctgg cgccatgctc accgccgcga tgaagacgaa
     2341 ggccgcgacc gacaaggcac tcgcgcagtg gtggtccacg ttcggcctgc cgacgcgtcg
     2401 cgatcaagag cgcgcgctgc acaagctcaa ccagctggag agccgcctct acgatctcga
     2461 agagcagctc cacgctgcca agaagaacta gtcatggacg tctcgcacct cctccaagtt
     2521 gctcccgctc cgcgcgctgt cttcgatcgc ctcgcgacgc atcgtcatcg cgtacgcttt
     2581 caagtgcgca aaggttcgtc gtgggagccc gtgacgtggg gccagttcgc gctgcagatc
     2641 cgcggcgtcg cgcgttggct cgtcgagcac gacttggaag cgggcgagcg catcgcgatc
     2701 tacggccata acagcgttgc gtgggcgtcg gccgcgctcg gtgcgcagac cgccggtgcg
     2761 gtgttcgtgc cgatctatcc ggcatcgacg cccgagcaag tcgcgtacat cgtcgagcac
     2821 gcggagatca aattcgtctt cgtcgctggc agagactgcc agtcgcgcct cgagcgcgcg
     2881 cttgcgatgg tgcccgtgaa gccgcaggtc gtcgacctcg aggatccggc attcgaagcc
     2941 gccggtgctc gcgacgctgc tgcacccacg ctcgtcgatg cgcgcctcgg tgcgctcgat
     3001 ctcgatcagc ccgcgcagat gctctacacg agcggcacat cgggcaaccc gaagggcgtg
     3061 ccgctcacgc atcgcaacgt cggcaagaac ggcgccgatt ggctcacgtg caacgcgccg
     3121 ctgctcacgg aaggcgatcg cgacctgttg tggctgccga tgagccacat ctttggcttc
     3181 ggcgagctgt gcgccggcaa tctccttggc tgggagacgt acatggcgac gcctgctgac
     3241 gtcctcgatc tcctgccggg cgttgcgccg caagtcttca tgagcgtgcc tgcctactgg
     3301 gagaagatcg cgcgcgcgat cgtcgccgga cccggcgcga ccgacgaggg agagcgcggc
     3361 gcagaagcgc gcaaggaggc gctgcggcgc gtgaccggtg ggcgcttgcg cttctgcctc
     3421 tccggcggtg caggcctcaa ggtcgagatc aagcaattgc tccatagctg cggcgtgctc
     3481 gtcatcgagg gctacggcct caccgaaacg tcgccgacgc tcacgctcaa tcgtccggac
     3541 gcgttccgct tcgacaccgt cggcaaaccg ctgccatccg tgcagctgaa gctcgacacc
     3601 gacggcgaga tcctcgcgaa gggcgagaac gtgttctcgg gctacttcaa ggatcccggt
     3661 gcgaccgccg cggcgttcac cgaagacggc tggtttcgga ccggcgacgt aggtcgttgg
     3721 accgacgacg gcttcctcca gattatcgat cgcaagaaag acatcctcgt cacggccggc
     3781 ggcaagaaca ttccgcccgc gaacatcgaa gcgaagttcg tcgacgatcc ggtgatcgag
     3841 cgcgtcgtcg tctacggcga ttcgcgtccg tacctggtgg ctgccgtgtg gctccgcccc
     3901 gacgtgccgc gcgacgaggc cctgcgtctc gcatcgcagc gtgtcgaaga gatcaacaag
     3961 gagctcgcgc gccacgagac gatcaagcgc cacttcgtcg ccgagctgcc gctcaccgtc
     4021 gaagaaggca cgctgacctc atcgctgaag ctgcgccgca agatcgtcta tgacaagctg
     4081 cgcgagcgct tcgaggcgct ttacgcctga ggcaccatga aagccccctt gaagaagacg
     4141 ccactcgata gcatcgtcgc gctgctgaag cgtgcaccga agggcccgcc gcccgtcggc
     4201 gtgacgccgc attcagtcgt gtggaccgag aacaagtggc gcctgctccg cttcgcgccg
     4261 gcgcagccgc ggttcgcgac gccgatcgtc atggtgccgt cgctgatcaa tcgctggtac
     4321 gtgctcgacc tcggaccgca gcgctcgctg atcgagtggc tcgtcgcgca aggccacgaa
     4381 gtgttctgca tcgattgggg cacgcccggc gccgaagatc gctacctcac gtgggacgac
     4441 atcgcaggtc gttacatcgg tcgcgctgtt cgcatcgccg cacgctacgg tcgcagcggt
     4501 cgcacgcacg tgctcggcta ctgcctcggc ggcacgcttg cggtcagcta cgtcgctgcg
     4561 ttccccgagt acgtcgcatc actgctcgcg ctcgccgcgc cgatcgactt cgagcacgcc
     4621 ggcatcatgt cgcaatggac gcgcacaccg acgttcgacg tccgcgcgat cctcgacgca
     4681 ttcggcaacg tgccgtggcc actgatgcag gcgagcttca acatgctgaa gccgacgctg
     4741 cgtgcggcca agaccgtcgc cctgctcgac cgcgcatggg acgacgaatt cctcgagggc
     4801 ttcctcgcga ccgagcactg gggtcacgac aacgtgtcgt tccccggcgc ctgctacgcg
     4861 cgctatatcg aagagctcta ccgcgcgaat cgcctgatga ccggtggttt caccgtgtcg
     4921 gggcgcccgg ccgagctgca caacatcaaa tgcccggtgc tcgcgctcgc gtttgccgac
     4981 gatcacatcg tcccgctgcc gagcgcatca ccactcgtcg atcgcgtcgc gagcggtgac
     5041 aagcagctcg tcgtgcagcg cggcggtcac gtaggcgcgg tcgtgtcgcg caaggcagcg
     5101 gaccggctgt ggcccgtcat gcacgacttc tgggccgcgc gctgactaga cgctgatcag
     5161 atcgaacaga ctttgtcgca ggccggcaga ccggtctcgt aattgaagtt cgcgtcgcga
     5221 acctgcgagc actcgagacc gtggatctga tcgacgcaat catcggcctg atcgccgtga
     5281 tacttctcgt tgccgtcgca cgtgttctca acggtctgcg ccgagcagcc atagtccgtc
     5341 tgatacttcg tgacgcaccc ggcttcgttc ggcggaaagc cgccagcggc tagctctgcc
     5401 gatgtgaagc aggcgtacag gcgctcgcag agcgctgcag cggactggtt gcaaccttcc
     5461 ttcggcgaca gatcgcctcc gccacacgca gcaagaccga ggaggataga agcaatgatc
     5521 gcgagtcgca tggcggcaga atgcctatgg ctgcatgctg cagtcaaccg atgaccgctg
     5581 cgaacgtcct cctgtcagcg ctgctgcacg cgctgtggaa cgcgctgctg cggctcgaga
     5641 aagacaagga tcgcgcgctc gttgcagccg tcagtgtcgc gacgctgctc gcgatcgtcg
     5701 tcggtggcac gcgctgggca ctcggcacgc tcccattcgc aacaacagag tccgtgatct
     5761 ggaccgtcgg tgcaggcgtg ctcgagtgga tctacttcgc gtcacttgcc aaggcactcg
     5821 atcgcgggcc gctcggcgcc gtgtacacga tctcgcgagg tggcgcgatc ctcgtcgtgt
     5881 ggccgatctc gatcgccctg ttcggcgagc cgctcgcgct cacgtcggca atcggcagcg
     5941 cggtcgtgct cggcggtctc gcgctcgcgg gcatcggtgc gggctcgacg aagcaaaccc
     6001 gcatcgctgc tctcgcatgg gcgatcgcat gcgcggtctc gatcgccggc tatcacctcg
     6061 cctacaaggg cgcgatggca gccggtggca gcgcgtcggc ggtcttcgcg gtcgcgctcg
     6121 cggtcgccac cgcgatcaat atcgtgcgcc tcggaacggc cggcagacgc atcgcgcgcg
     6181 agctcgtgca acagcgcccg ggccgcgtga tcctgatggg cgtcatctgc ggcggctcgt
     6241 tcctcattct catggaggcg ctgcgtggcg gaggagccgg ctacgtgctg acgctgcgca
     6301 atacgtcggt gctattcgcg acgctgttcg ctgcgctcat cggcgagcgg ccgcgacgtc
     6361 ccgaagtgct cggctcgctc ctcgtcgcga tcggcgccgt gctgatggcg tggcactaga
     6421 agcgcagcgc cggctcgtct tcggcaaacg cctcgcagac gtcgacgtaa tcgatcggaa
     6481 tcgcgagccg accagcctcg ccgaacgggt tctgccacgc agtgcccgcg tcgtagaaga
     6541 agttgaagtc cgccttcacg atgtacgagg tcgcgacgtc gagcgtgaag ccgacgtatc
     6601 ccgcgccgtc ggccttgggc tgccagtccg tgaaggtgac gtccctcacc gtgtcgattc
     6661 cgcgcacctg caccgtgagg cgctcgccga ggtccttacc ctcgacgcgc gtgcacgtga
     6721 ccgcatcttc ctgcgcgccg acgtccacct gtccatctac cgcgcagccg ccgagcagga
     6781 tcacgaggag gtaacgccgc atgcccgtcc tcagttcaag tacgatgccg gtggatgttg
     6841 cgtagggtgt ggctcgcgct cgcggtgacg gggtgcaagc tgcacttcga cgacagccat
     6901 gacgcggcta ccaccgagcg cccgtgcccg gacgacttca tcttcgtgac gggcaacccc
     6961 gcgttaggta ccagtgactt ctgcgcgatg aagttcgagg cgaaggcgcg cgacctcggg
     7021 acgcagcaga ttgtcgcgaa cggctgcaat gcgacgtgcg atcccgattg gggctcgacg
     7081 ggctacggcg cggcctccgc gagcgaaggc gcaccatttc gctcggtcaa cgcgctcgac
     7141 gcgcagcgta tgtgtcgcga gctcggcact ggctacgatc tgatggcgaa ccgtgagtgg
     7201 atgacgatcg cgcgtcaagc cgaggcgaat ccggcgaact ggcaatcggg cacacccggc
     7261 actggccgca tcgctcaagg caacacgaac ggctcgacgc ctggacagct ggcctgtacc
     7321 gacgagagcg atccctataa cggcaccggc gatgacgcga tgacggcatg ggaccagcgc
     7381 cgcatcgagg tcctcgcgaa cggcgaggtg atctgggact ttgcaggcaa cgtccaggag
     7441 tggatcgatt tcacgctcgc tggtccctac gatccgccgc cctcgtgctc gagcagcgag
     7501 ctgccaacgg tctcttgcgc cgggctctcg cgtgacgact ttcaatcgac ggccggtacg
     7561 ctcgattcga cgaatggtgt cggcaagctc atcggcggct cgggcaacgc gctgcggcga
     7621 ggcggccaga cgggtgactt cgccggcgac ttcgcgggca tctacgcgct caacatgaac
     7681 cgtttcacca acgacacgtt cccggcgact ggctttcgct gcgtattgcg cctctgatat
     7741 gcacgcctca tgagactcga gcacgttcgc agagctgtcg cgttcggtgc acaccccgat
     7801 gatgtcgagg tcggcgcagg cggtctcgtc gcgaagctca cgaacggtgg cgcgcatgtc
     7861 gccatcgtcg tgacgtcgat cccgaatcag tacgcagttc gtcgcgcgga agccacggca
     7921 tcggcggcga agctcggagc cgcgctcgtg ctgccacccg gcgatgagac aggctcgcgg
     7981 atcgaggacg tgccgatgag cgcgctcgtc gctcgcttcg aacgcgagct cgcgtctgtc
     8041 gagccggacc tcgtcatcgt ccatggctcg cacgactcgc atcacgatca catcgcggtc
     8101 catcgcgcgg tcctcgccgc tctgcgccgc acgcgttgcg acatcctggc gtttgcgacg
     8161 cgactgcccg ccggtgcagc tcctccgccg ccgacgtgcg tcgtcgatat ttcctcgacg
     8221 atcgagcaga agctcgctgc cgtcgccgag cacaagtcgc agttctcagc ggacttcccg
     8281 gagaagcggc gcgacattca ccgcgcgctc ggcatcacgc acggcatgca gtacgccgaa
     8341 gtctacgaag tactacgcat cgagctgtag ctactcggtc ttgccgaact cgacggcagc
     8401 tgctgcgagc gcgagcagcc ccaccgccat cacgatcgcg agctcgagca ccgtcggcac
     8461 ttgccagccc gaccacgtga caccgggcac cagcgatttg tcgacgaact ggccgagcac
     8521 ctcgcgccgc atcggatcga cggcgtacgt gagcgggtcg agcagcgtcg cgatgtgcag
     8581 ccaggtcggg aggcccttca tcgggaacag cgcacctgac aggaagaaca tcggcatgat
     8641 gatgatctgg acgagcgcca tgaacgcttg gaacgacttg atccgcacgg cgacgagtac
     8701 gccgatcgac gtgatcgcaa aggacagcac cgcgagctcc gcgagcagcg tgagcacgag
     8761 cagcggcgtg aagtgcacac ccacgagcgg ccccatcgcg agcaccacga cgccttggaa
     8821 catgcccgcg gtcgcgccgc cgagcgcttt tccgacgacg atcgagtagc gccgcaccgg
     8881 agcgaccagc atctcgcgca tgaagccgaa ctcgcgatcc cagacgatcg agccggccga
     8941 gaagaagcac gtgaacagcg tcgacatcgc catcacgccc gggaacaaga acacgcgcag
     9001 atcgagcccg cctgtcggcc gcatgccggc gcccatgccg gtgccgaaga cgaacaggta
     9061 gagcaccggc tgcaggatcg acgcgagcat gcgcggcttg tcacgccaga accggatcac
     9121 gtcgcgatgc cacacgatgc gaatcgcgcg gatgtcgctg cgccagctgc ggggcgggac
     9181 cgtgacggca acgggctcga gcgtaccggc agcggccatc gctagcgtcc cctcgcggcg
     9241 cgcgcgaact tcgccatgcc gctcttctcg aaagcgcttg cttcggcgtc gcggatcgtc
     9301 gagccggtgt acgcgaggaa gacgtcgtcg agggtcggcc gtgcgacgga gaccgagcga
     9361 atcgggatgc cgagctccgc gaacaggcgc ggcaagaatt gttcgccgtg ctcgacgttg
     9421 aacatcacca tgccttcgcg cttcgccgcg tcgatctgga acttgtcctt cagcgcgcta
     9481 atcgccgcgg cgtcgtcggc ggtagcgatc tggacgcggt ctttgccgac ctttgccttc
     9541 agcgcctcgg gcgtgccgag cgcgacgatc ttgccgttat ccatgatcgc gatgcgatcg
     9601 cagtactcgg cctcatccat gtagtgcgtc gtcaggaaga tcgtgatcgt ctcgcgctgc
     9661 ttcagctcgc ggatgtactt ccagatcgac gcgcgcgtct gtggatccag gccgaccgta
     9721 ggctcgtcga ggaacaacac gcgaggcgag tgcatgagac cgcgcgcgat ctcgagccgc
     9781 cgcttcatgc cgccggagaa tgtctgcacg cgatgggtgc ggcgctccca tagaccgacc
     9841 atctccatca cctgctgcat gcgcggcgcg acgcttcccg acggaatgcc gtagagctcg
     9901 gcgtgaaagc gaaggttctg ctccgccgtc aggtagtcgt cgagcgtcgt ctcctgaaag
     9961 acgagcccga tgtggcggcg cacttcgtcg cgctcactca tcacgtcgtg gccagcgact
    10021 ttcgcttcgc ccgcagtggg cttcgcgagc gtgcacagaa tcttgatcgt cgtcgacttg
    10081 ccggcgccgt tcggcccgag gaagccgaag acctcaccgg ccgcgacctc gagatcaatg
    10141 ccgcgcaccg cctcgatgtc gccgtagcgc ttctgcagac cgtgaacaga gactgcgggc
    10201 gctgtcaccc gcgcagctta cggcagctcg tcaccttgca gccagccatc gccgtctcga
    10261 tcgagcggcc gcaattcatc tgccagcgca ggcggcattt cgcctggacc tacgttgccg
    10321 tcgttgttcg cgtcgtaatg gcgaatgaac ttgctgcgac gctgcgcgcg gtcgtccgcc
    10381 ttcgccaacc ggttggcgag cttgtgcaac accttcgccg catgcttgcg ctcgcgtggc
    10441 ccgagcttgc cgtcgtggtt gcgatcgaac ttctgcaaga gcagctgctt cagcgggcgc
    10501 ttctgctgcg gcgcatccgc gtgagcaata ccggtcagac cgacgacgag cgcaatgacg
    10561 gcgagcttca tggccatacg acccgcggcg cgccgaaagg ttaaacgcgg gtacgacgac
    10621 gacggcgacg caagagcaac atcccgaccg ctcccgcgag cgtgatcgat ccaccaccgc
    10681 cgctcgccga gcagcagccc gtatcgacgg gctcgatgat cttgcacatc gacgagcagg
    10741 tgtctgtgag ggcgccgtcg tcgcattcct cgtcggagtc gatgatcgag tcaccgcatg
    10801 tcgggagatg gcagtcggtg tgacaggtgt ccgccacatc ggcattcatc ccgccgttgt
    10861 cacattgctc ggtccgcgtg cgcacgccgt cgccgcagat cgcgtacggg ttcgcgacga
    10921 tgcagacgtc atcgatgtgc cagccaccga gctcgaggcc cgcgtcggac gcgagatcga
    10981 acgtgacttg cgcggtgtgc ccggagatgt agccggagag cggcacgtcg tggaaccgcc
    11041 actcgcgatc gacgtgatgg atcgcgctcg aatcgccgag gtccgagtcg tagttctgcc
    11101 aggccttctt tccgttggcc atgatctgcg ccttgtcgaa gtgcccatcc tcgacggcga
    11161 gccagcggcg atagtgcaag cgcacgtcgc tgtactggcc gatatcaatg acaggcgtct
    11221 tcagccacgc atcctgctcg gcgttgtaat cgccgtcgag cttttgcgcg acgatcttcg
    11281 agcctgagaa cgcagcgtgt gggtccgtcg gtcccgtcgt aggcgtgccc cactcgaacg
    11341 gcgacgcgcc ctctttatcg gtgcccgtcg tccagccctc cgcgaacgga tcggtcgact
    11401 cgaagtcggt gcagtacagc ttcaccgttc gcccttgata gagctggtag tacggatcgg
    11461 cgaggttgtc ggcgagcgtc atcgacgagc cgtccgcgaa cttcacgcgc gccttgtagt
    11521 agatcgattc ttgctgggcg agcggcagct gcgcgaagaa cgcgttcggg ccggcaggcg
    11581 tcgcttccgc cgttcctgcg tgcggctcgc cgaacgaggg cacccagtcg atgaacgcgg
    11641 actggacttg atcgaggttg caacggtcgg acaagccgct gacttggatc tgaacgccga
    11701 tcgcgagcgc cggaatcgtc agctggccag gcgcgacgac gcgtccagac gcggtccgca
    11761 gaccgtgacg cccgaacgcc gcgcgaatcg tgcactcgtg cggcgtgcca ttcgacaggt
    11821 cgccatcgtc gtcgtcttcc gcgagcactt cgacgagcgc cgtcgggatg ctgaccgcgc
    11881 gccgaagcgc tgcgacatag agccggttcg tgagctggat tcccggggct tctccgaggt
    11941 ccgcgatgag acccttgcgc aggtcccaaa acgcaccgcc gaagatcatg ccggtgtggt
    12001 ggatttcgcc gatgtcctgc ggccacagcc actctttgtc gtcgggatcg agatcgcgaa
    12061 gggcttcgct ggtgcggaag aagccgcgcc ccataccgtg atcgccggtg atcgtcgcgg
    12121 cgagaaagtc cgcggcgcct tcgctcatcg caccgtcgaa atcgccaacg ccgtcgatga
    12181 tctcggcggt gtgaacgcgg tgtccgtact cgtgatagac gacgtcttga atgagcgccg
    12241 tgttctgaca ctgcatgttc gactggtaga agttgatcgc tttaccgtcg aagaatgcgt
    12301 tgcagctgtt cgcgatgttg acgttgaccg gaatgacttc atcgagcgtc cgcatgttcg
    12361 cgtcgatgtt cgcgcgcacg tagtccttcg cgatcatcgt cgcgacgtac gcgtttacct
    12421 gcgcgtcctc ggattcgctt ccgctcgcgt cccacgcgaa ctggctgccc ggctgcagtg
    12481 cgtagagcgc ggtcgcctcc gcgttgcccg tcgccttgtt cacgaccgcg gcgagatcgc
    12541 cggtgatgcc cgtcgcgacc gaggcctcgc cgtcggacca gctgacgacg cccgtcgcgc
    12601 tcgtcgtctg cgcggcgccg ttaaccgtga cgtgtgcgcg cttcgcagga agatcaagcc
    12661 ggcccctctc gggatagcga tcgacgacgt gataattcac gacgccggtc gcgtacgcat
    12721 tgagctggcg cagcgcgagg atgtcgccag tgctcgcatc cgcgtagccg acgtagcgac
    12781 ctgcaccgcc gccgtcgatc gtaacgggac gcgcgagccg atagccgaga accgcatcgt
    12841 cggcgacgag cggcagaatc acgacgtcgc ctggctgcgt gaccggcgcg ttcggcaagg
    12901 cgacggcggt gcgcaaatgc tccgtcgcgc ggcgctgcag atcgcgtggt gcgagcttcg
    12961 ccttcgtcgt cgccacgctc acgttcggca gcgcttcgga gccgatcagg aacaggcgat
    13021 cagctttgaa gcggaagctg atttggcccc cgatgacgtg aatgccgccg ctgcgctgga
    13081 tgaagccgag cgtgcgcatc gagccgtcga agtgattgct cacgagctcg aagtcgctcg
    13141 gctgcgagcc gggtgcgaag agcgcgagat gatccgcgag cacctgacgc gcgatgcgct
    13201 ccgccgtcgc tggatcggca acggagccgg gcgcggcaat accaggtccc cacatgcgag
    13261 atggcacacc ggtcgcgcga tcccagccgg cttgccagcc agtgtgacgc tgcgcgaacg
    13321 tcgaccatgc tgcagacggt gcttgcgccc actcgagctt gcgttcgacg cgcaacggct
    13381 tcgcggttcc cgcggccggc atggcggtca gtgcgtcggc gctgatctgc ggtccctgtg
    13441 tcgggacgac cgcatgcgcc tcgagcgatg cgagcaagca gccggctacc accagccggc
    13501 gggcacgaac gaccatagag ccgacattag agcgcattcg cggcccggcg cgtagcgacg
    13561 attgtctcgg ttaaccgctg acagcagcta gcgcttcgcc ccgggcggac ctcacttgga
    13621 ggcctagctg cagctgccct cgggctctca cgaggttatg cgcgcgccgg gacgggaacc
    13681 gctcggcgtc ccagccaaag tagcgatcct cgaagacgtc gacggcgaac ctcgcggcca
    13741 gctcgataca gacggtctcg agccccgtga cgatcgacac gcgctcctcg gtcgtcagca
    13801 ggtcgttggc ctccgcgcga aagcccgcga tggccgcgtg aaagatcgac aggtcgaatc
    13861 gcacgctgcc ggcgtcttca ccgtgggggt tgcaccacga ccgcatcgca tcgccgagct
    13921 cgaatgccat cgtgcccttc gcgaacgtgt cgagatcgac gaggcaaacg ccggcgagcg
    13981 gctcccgctg gaagatcaag ttcgagatct tgaggtcacc gtgcacgtgc cgcttcggcg
    14041 cgtccggcag aacgggcagc ccgcgcgccg cgcgcagcac ttcgcggccg agctccatcg
    14101 cttcgacatc gccgccggct tcgagggcac cctgtagctt gctcaagtgg cgcgacgtgt
    14161 cgtgcacgcc ggagcgcgtg aacttgtagt cgtactgcag atccgcaatc gcgcgatgga
    14221 agcgaccgac gagcgcgccg cccgcagtgg cccactcgac cgacggcacg cgatgcaccg
    14281 tctcgccttc gacgtacgtc agcgcgcgcc agacgtgacc gacgtgatcg acccatgcgt
    14341 tgccagcgag cgtccggatc aggcgcggcg tcggcagacc gcgcgatgcg agatgcgtcg
    14401 tgacggcttc gatgtcgagg ttcacggcag cgccgaagat cgggtgcagc tgctgcagca
    14461 cggcgatacg cgcgtcacct tcggagatga cgtacgtctt gttgatcaga ccaccagcga
    14521 gcggcgccac ttggtccgca cgccagcccc acgcttcagc gacctgctcg aggctcactc
    14581 tagtgatgac ctggcggcat gtcggcatgc ggcgcagcag gcttgatctc gaggagctcg
    14641 atgtcgaaca cgagcatgcc ctgcggcctg ccctgctgac ctttgtacgc gaggtcgtct
    14701 ggaatccaga accggaaccg atcgccgacg gacatctgct gcagaccatc cgtccagccc
    14761 gcgatcacgc cttgcaagct gaactcgctc ggctcgccgc gaatcaccga cgagtcgaac
    14821 atgcggccgt cggtttgcca gccggtgtag tgcacgcgaa cggtatccgt cggcttcggc
    14881 ttgtcgccgc ccttgccggc cttgagcact ttgtagaaca cgcccttcgc ggtcttcctc
    14941 gcgtcgccgg gaggcttcgc gacatcgggc ggtgacggcg gcggatcgcc cttggctttg
    15001 tcgatctgga gcacctcgac ttcgatcacg acgggccccg tcacgaccgt cttcacttgc
    15061 tccttgagcg ccgctgcatc gacccacgct cgcacgcgct cgcccggcac catctgttcg
    15121 aggatttctt cgaacgcgac ggcttgcttg tacggcgtcg agcgcgcggg ttgcttcttc
    15181 atctccgtcg tctcgatcat ctttccttcg gcgtcccaca ccgtgtagtg gaacgtcact
    15241 tcgtcggcga tgtgcgcggc gtccttgccg gtgccttgcc gaacgacgac gtacttcgtg
    15301 ccgctcttct tcgtcgagag cgcgtcgggt ggcgggcctt tcacatcggc gggaacggcc
    15361 ggcgcttcct tgatgtcgac gacctcgact tcatagacga gagtctcggg attcgccggc
    15421 ggcggcttgc catcctcgta gccgatcgcg gcgggcaccc agaacatcgc cttctcgcct
    15481 ttgtggacga gctgcatccc ctcggtgaag cccttcgcgg ccttcgcgag attgagcggc
    15541 atcggcgact cgctgttgat gttcgagaag aacgtttcgc cgctcgcttg ccgccaaccg
    15601 gtgtacttca ccatcaccgt gtcgtttcgc ttcggcagcg ggcccgtcgg gtttggcttg
    15661 atcggcttgt agatcaggcc cgacgcggtc ttcaccgcat cccccggcgg gttcttcaga
    15721 tcgaacggcg catcgacctg cggtgcagct ttcgccgcca cgccgggacc gctcgtgacc
    15781 tttgaattgt cctgctgcgc ttccttcttg cagccaaccg ttccgagcaa tgcaacgaga
    15841 atgacagctc tcatgccttg taggcctcgc gggtcaggtt ccgaattccg tccgcctcca
    15901 caaccacatt gtcctcgatc cgaattccac caaatgggcg gagctcgtca acgagcttcc
    15961 agtcgatgag cggacctcgc tcggcatcct cgcgcagagg gccgacgagc gcatcgatga
    16021 agtagatgcc gggctcgatc gtgaacacgt ggccgacctc gatcgtcgac gtgttccgca
    16081 ggaatttgtt ctcggggcgc ggcggccgca gcttcatccc gacgtcatgc acggtcacgc
    16141 cgagcgagtg gccgagacca tgcgggaaca gcgcgcgcgt gatgcccttc gcgacgagct
    16201 cttggcccga cgccttgccg atgccgacgt ccttcagcat gtcgccgagg agccgatgcg
    16261 attcgtcatg cagctcttcg tactgcagcc ccggcttgat ccgcttcagc acatcctgct
    16321 gcagctgatc catccgcgcg atcagctcgc cgaaacgctt cgcgggaccg ctcgtgccac
    16381 gcacgtacga gcgcgtgatg tcgctgccgt acccgttgca gcgcgcgccc gcgtcgacga
    16441 gcagcgacgt gtcgccgctg accttcttgc gctcgtacgc gacgtagtgc aggaccgccg
    16501 agtgcgcgcc gagtgcgacg atgttcttgt acggcgtcgt cggatcatcc tgctcgctcg
    16561 cgtccaagta cgtcaggtgc agctcgagct ccgacgcatc cgtcgtctgg aactttttct
    16621 ccgtcgcctt gtgaccacgc accgcgcgcc ggctcgcgat cgcgaggcac tcgatctcgt
    16681 acgcagtctt gcgcgtgcgc gtaccgtcga gcgcagcgat cagcgtcgca ggattcacct
    16741 cgcccggagg cgcctgcccc ggatcgcgcg tgacgaccgc gaccttgccg ccgggcagca
    16801 cgtcgccggc atggccgggc ccgacggtca cgacatcgaa ctgttcccag aagtgctcgc
    16861 tctccggcgg cgggacggtc tcccaaaagt cgtcgaccac ggtccgcacg agcgtcggct
    16921 tgcggccctg ccgcacgacc acatacgagt ccgcctcgac aagcgggcac caatgcgtga
    16981 acgccggcgt cggcatgagc ggccacgact gatcatcaaa gcggtttcgc gactgcggcg
    17041 cgccgctgca caggatcagc gcttcgtagc cctgatccgc gaggatcttc tcggtcgcgc
    17101 gctggaggtt cgcgacgtgg tccgggtagt tgtcgaagct cacgcgccgg aatctagcgc
    17161 ggtttcgtgg cgaaggccgt gaccggggtg cgctcgatcg cgaacgttcg gccctcgaca
    17221 aggtcgccta ccaacaggcg aattgccggg agggcgagcg gaccacgttt ggcgggcacc
    17281 gtgaagcgac gcgcgacgga cttgatggcg tcggggtcgg ccgccttgaa gccggagacg
    17341 gtggcgtcgc ccttgaagct cagcgccgcg ttcgtgatcg acgtcttgtc cgcagcggac
    17401 agcgtgccgt atgcgacgag gaccggcccg gccagtgcgg gaagctcgag cacgacagcg
    17461 gcgcccgcgg ggaggtccgt gccgcgcggc acgacgacgg cgtcgacctt gccgagacca
    17521 agcgccgcga gcgcggagac ggtgtcgggg gcggtctcga tctttgcgaa gtagtccttc
    17581 gcgacttcgc cgccgaggag cacgttgagc acgagctcgg tttcgcggcc accgatcgcc
    17641 gggacgagca cgcgcttgcc tttgagaccg gcgagcttgt ccgcaccacg cgcgacgatc
    17701 tgccagccgt gaacgctctc gccgttgcgc gtcgcggatg ccagcagcgt gtagttgccg
    17761 cccgcgacgg cgaggtatgc cgcatcgacg acggcgacgc tgacctcgcc cttcttcgcg
    17821 gccgcggcga agtcggcgcc acgtgcgaac actttgccgc tgccggtctt gttgatcgag
    17881 ttgccgaggt gctcggcgag gcgcgacgcg agctcgacac gagcggacgt cgacgggaac
    17941 ggcgcggtcg gcgcgaacag accgacggtg aagccttcgg cgcgcgcgat cgccggcgat
    18001 gcgacgagca gcacgagagc gagcgcgagg tgcctcatgg cgcttcacct ccgtagacgc
    18061 gttccttcgc ctcgatccac tcggtgagcg gtgcgaaccg cacgttcatc ttccgcgcct
    18121 tgcgccacgc atcgagcgcg cgcgcgtggt cgcccttttt ctcgtaggcg atgccgaggt
    18181 tgacccacgc ttccggcacg cgcgtcgtca gcttctcgaa ttgcgcgatc gcgacgtcga
    18241 ccttgccgtc gacgagatcg aggactgcaa ggttgtgcgc aacttcgtcg gtgccgacgc
    18301 gcgcagtcgc cgtcttcgcg gtcgctagat acttgcgagc cggcgcgagc tggccgccgc
    18361 gataagcgtc ttgcgcagcg ttgagggcga cgacgcgaat cgatgtgccg agcagctggg
    18421 cggcgatgcc cgtcgacttg ccgccgagcg aggtgagctt tgtcagcgca ttcggcgcct
    18481 tcttcgcgtt gagaccgtcg gtaaacgcga tgaggatcgg cactgcttgc gtatcggccg
    18541 gtggcgggaa tggacaggtc tggccctgca cggcgcgcag cgcggcgagc gcagcagcgt
    18601 tatcaccagc gacgacggca gcgacggcga gatcgcactt gcgcgcgagc gttgcttcac
    18661 cgggtgtcgc ggcggcgcgc agcagctcga cggccttact gccgttgcct gcgcgaagct
    18721 gcgcgagacc tgccgcgtga cgcgccgatg cgagtgctgc tttgtgacga gccgcgagtg
    18781 gcccctttgc ggcagcggtc gccgtcttct cgagcgcagc aaccgcgagc gccggatcac
    18841 caccggcgac ttccgatgct gcccagtcga tcgcgatctc caggttgtct ttgtcgccag
    18901 cgagcgcacg ttcgtagagc ggccgcgcac cagcgatgtc accggcaacc gcgtgcgcgc
    18961 gagcagcgag catcgtgacg accggcgagg catcggcctt cgcggccttg tcgaggatcg
    19021 cgaccgcgtc ggtcttcttg tcgagcgcca agcgcgcgat cccgaggttg cgccagacga
    19081 gcggatttcc gtcgaggcca tcggcccgca tcagcagcgg ctcggcgccc gcggcgtcgt
    19141 tcgcagcgag cttgtccgcg gcgaccgtcg tcaacgcttc ggacagcagc ttgcgcgagc
    19201 gaggcgtcga ttggatcgct tcggcttcat cgaggagctt tacggcgtcc tcgggtttgt
    19261 tcgtcgcgag caatgcggtc gcgagcacga tgcgcgtcgg cgcgtgggtc ggcagctccg
    19321 cgacgacagg cgtgagccgc gcgacgacct tgtcggcggc gttttgcgcg agcagcgtct
    19381 cgccgagctc ggaccaccaa tcgggatcgg cggatgccgg cgccggcggc cccagcttct
    19441 cgagaacggc ttgcgcatcg gccgccgcgt ttgcgcgacg aagcaccacg gcgaggcgca
    19501 cggtccagcg ccggcgctgc ggttcgaggc tcgcagcaag ctccagctct ttgcgtgcgt
    19561 cgggcagatt gccgcgcgca gcctgcgctt caccgacgag tgcgtgccct gccgccgagc
    19621 ccttcgcctt ctccgccgcg acgcgatcga ggtagcgacg gccgagtgcg agcgcgccgt
    19681 tcggatcgcc gagcgcgagc gcgcattgac cgtcgacgag ccagatctcc gcgtagccct
    19741 gttcggcgat cggctcgagc tccttcagca tgccacgcgc cttcttgcag tcgatcgcgg
    19801 cggtcgacca cgcgatgccg atgcgtgcgc gcaggtcggc tttcggattg gccttcgcgg
    19861 cgtgctcgaa cagcggcagc gccttcgacg gttgccccga tcgcaagtac gaatccgcga
    19921 gctcgacgcg gtagcgcgga tcttccttgg cgagctgctg cgggcgatgc gtgaagtaca
    19981 gctcgagcgc ggtgatcgat tcgggccacc tgctcgcgtc gtggtagtag cggccgagta
    20041 gcagctgcgc gtcgtagcgt gcggactctt tgctcgcgac ttgctccaag tacggcacgc
    20101 cggcattgac gtcgcccgag agcacgagct gcgagccgag cgccgtgagg acgtcgctgt
    20161 cctgcggggc gagcagctgc gccttgcgca tgagcgcgat acccgcggtg tattgcttgc
    20221 gcgctgcata gaccgcggag cggagcacgt acgcttcaac gcgacgagga ttgagcgaca
    20281 gcgcttgatc gagcgatctc gctgcatcgt cgagctcacc tcgtcgcagt gccgcgcggc
    20341 cttcttcgac gaacgcgacg ctctcgtctt cgccctgatt tgcgcgttgc gcggctgcag
    20401 tcgcagcgag agcgcagacg aggctaatag cgatagccga ccttgacata aatctctctg
    20461 ccctcgccgg gcacaagtgg aatcgtcgtc gtcgacatcg tggtctcgtc gtagcgatcg
    20521 tagtcgccgg gcgcgacgat gcgctggcgc ttgccgatga tgttgcgcac acccgcggtc
    20581 agatcgaagc cgcgcaggtc aggcacgtat acgctcccat tccagccgac ccacgccggt
    20641 gccggatcga gcgcatcgcc ggtgtccacg tccggacgag ttggcctctc gccgacgaag
    20701 atcgcttcgc tcgacaggtg gacgtagttg ccgagcttcg gggtcgagac tccgacggag
    20761 ccgatgatcg cgggcgcgtt cggcacctcg ccgaaggtca gcggcgcggt ctcgctctcc
    20821 gctccgactt gttgatagct gaggccgcca aagctgtacc aaccggcggc attgcgatag
    20881 ctcgcctcga cctcgacgcc gcgcgtgacg aagcgatctg cgttctgaaa ctgcagctga
    20941 tccatccccg tcacgggatc cgtgaacggc agctgctcga cgacatctcg cgcgtcccaa
    21001 tagaacccag acagacgcgt cgagagaccc gcgaccggct tcgcccatgc gacgacctca
    21061 cccgagtaga tgcgctccga accgatgtcc gggttcgcgg caaagctcgt ctgatcggtg
    21121 aagaagccct cgaacgcgct cgggttgcgg aagccctgcg cgtagaggag cttcaggccg
    21181 tacttctcgt gcgtgctgag aaacagtgcc gcgcgcggcg acaggctgcg gtcgatcttc
    21241 gtgttctggt cgaatcgcag gcccgcggtg tagccgagcc aatcggtcgg ctgaccgtcg
    21301 agctcggtgt agacaccgac caccgcgaag tcgagtggca cttcggcggg ctcgctcgtt
    21361 ccgtcgagct gctgcgcgac gcttcgcgtg acgttgtagt tcgcctcggt acctgctgtg
    21421 acgccgagct tgttcggcat gacgagctcg tagcgaccgc gaacctctgc accgaacgtc
    21481 ttcgcttcgc ccaagtcatc gaacgggatg ccgttgatct ggttgatgtg atcggtgaag
    21541 cgatacaggt ttgcgtagcc gcgacctgcg agcgtgaacc gcttcgagag ctcccgcgtg
    21601 tgcccaccct cgacgagcag ctgcttgtcg tactggttgt acggatcggg atccgccgga
    21661 tcgccgttgt acggggcgaa tggcgagtcg cgccgatatt gcacggcacg cacctgcgcg
    21721 aagctgcctt gatagctgcc gacgagcgac agcatgtact gatgggaggc gtcgccctcg
    21781 aggccgcttc cgacatcagg caccgacgat gtgtcgccga gccggttcat ctgctggaac
    21841 gtgccgcgca gctgctgatg cacgtcgccg gtcgcgaagc cggcgctcgt ggtgacgccc
    21901 cagatcgagt tgatcgacgc cttcgcccac gcacgaggcg tctccgccgc gctgcgcgtg
    21961 acgatgttga cgatgccgaa gaacgcgttc gatccgtaca gcgacgagac cggcccgcga
    22021 atcacctcga tgcgcgcgac gtcgtcgatc gagacgaagt tgtcgaacga cagcccggcg
    22081 aagcttcccc acccttcatt gacgctcgcg ccgtcgacga ggacgaggat gcgcgtgttg
    22141 aaatcgcccg gcacctgcat cccgcggatg ccgacggagt acgagatgcg attgtcggag
    22201 atgtacacgc ccgcgacgcc agccaccgct tcaccgacgg tgcgatagcc aaagcgtcgc
    22261 agtcgatcgc cgctgacgac cgtgacggcc gatgccacgt tgccgaggct ctgctcgcgc
    22321 ttcgctgcac cgacgacaac gtcctcctcg acttgcgacg ccgcgagcag cgtggccgag
    22381 ctcgattcct cctcggacgc aggcggcagc ggcggcaaat cttggacgtc gacgtacgca
    22441 ccttgcgcat gtgcagcgcg cgtcgcgagc aggagcgcga gagcaagcgc tcgcttcacg
    22501 cggctcctcg cccgatgcca gtgcccgaga cggtgctgct gcgcgccggc ggatcgaact
    22561 ggcagagcac gaacgtgatg tcgtcgtcgg cttgcttgcc atcggcgtga cgctcgagcg
    22621 cggcgagcac ggtgtcgcgc aacatgatga ggccgcggtc gtcctgcagc tgttgcccgg
    22681 tcagcgcgct ccgcagccgg cgatcgccga acagcttgcc cgcgggattc gcgcgctcga
    22741 cgacaccgtc ggtgaagcac acgaacaagt cacccgcgcg catctgcagc gagccccgcc
    22801 ggatctcgac cgcgatgttg cgatcgccga gcggattacc actcgcggca atgatgctcg
    22861 cgcgctcgag cacacgcgta ttgccgagcg tgatcacata cgggaagttc tgccccgcgt
    22921 tcgcgtagtg cagcgcgccg gtctgcgaat cgaacacggc agcaaatgcc gtcatgagca
    22981 cgttgtgatc gccgaccatg cgaatcgcgt gatcgatcgc gcgcaagact tgttccggcg
    23041 tgagcaaccg ctcgtcgacg gcagcgagcg cttcgacagc accgcgcgcg gtcgcggcga
    23101 tcatcgccga gtgaatgcca tggccggtcg cgtcgccgag gacgagcagc atgcgaccgc
    23161 cggatagctt gcgatacatc caccaatcgc cgccgcactg cgatgcaggt tggcagtagc
    23221 cgatgacctt cagatagcca tgctggtcga ggttcatcgg cggcaacatc gcctgctgca
    23281 cctgccgcgc gagctccatc tccttttcga gcgagacctt ctgccgctgc tcggcgagca
    23341 agtcgacgat ctcgtcggcc atgaagttga acgtgcgcgc gagcacgccg agctcgtcac
    23401 gccgcgtcgc aggcacgcgg ctcttcagat cacccgacgc gatcttcttc gcctggaccg
    23461 tgagctgctt gatcggtcgc gcgaggctca cgccttggag cgcggcgaac acgacgccga
    23521 tgccgagcac gagcagcgcg atcatcagca gacgctgttg cgacgccttg ctcttctcgt
    23581 cggcagtcgc gatcgacttc tgcagctccg cctcgagtcc cgcggtcgtc acgccaatgc
    23641 gcagcgcacc gactgcgttg ttgccgagcg tgatcggcgc gccgtagacc caatcggcgc
    23701 caccgcccac gtgtgcgtgc acgacgtcgc cgtctttgat cgcgccgggg ggcgccgctc
    23761 gcgatcagct tctcgatctc gggcagcttc tcggcggccg gcgccttctg cgttgccgca
    23821 acaacctgcc ccgtcgtatc cgtgacgatc agccactgca ggcggtcgtc gccgctggcg
    23881 tgatcgtcgc gcatcgccgc atcgagcacc ggcttgatat cgggatacgt cgactgcgcg
    23941 agaggaagcg ccaccgcggt tgcgacggcc tggaccacca ggttcgactc gcgcgtgatc
    24001 gacttctcgc cggcgaagcg acgcgcgctg atctgcgtcc gcgtgaggtc atcgatggtc
    24061 cgctggctga accacgtcgc tgcgccgacc gcgaccgcca cgacgaccga cgtcgtgagg
    24121 atcaatttcg ccgagattgg aatcccggcg cggccctgag cgggaggggc gctcacctac
    24181 cccgagttta accgattcgc ctgcccatcg actgcacccg ctccactgca actgcaaata
    24241 accgttcgag acctgccgca ggttcttcac cttcgagcag ccgaagcgct cgcgcgatcg
    24301 cttcgatcgt tgacacgcgg cccggacccg gcgacgcacg cagacgctcg gcagccatcg
    24361 gctcgaccgg cagccgcaac atcggcagcc cgcgcagcga atcgatcttg cggtacatcc
    24421 ggcgcgcttg cgaccacgtc gcatccagca cgacgagctg cttgggcggt ggcgtgggac
    24481 acaccgttgt cacctcgccc tcggggaaca ggagccacgc gccatcgagc ccctcgagct
    24541 tcgccggacc tgcgctaccg ccgtgctcga cgatctcgct gttcgtcagc gcgaggttcg
    24601 cgagccgacc ggagttcgac gtgcgagcac gctcgagatg atgccggacg atcacgacac
    24661 gcgttcgcgt cgcgatcacc ggcacctccg cgcacagaca cacccgcagc tgcatgaggc
    24721 agcgcgggca gcgcgtcacg tcacctgcag cagcggaagc cgatgttggg gaagaagaag
    24781 gtgtcatccg cgagcgtgaa gttcagcgga cagctgatgc cgtctgccgt tccgttcgag
    24841 gcgccacctc ggatcgggtt ctcgccaggc gagtgcgcga gtgtccactc gcgaacgttg
    24901 cccgacatat cgaacacacc ggtgcccgta ttggcaacgc agctcgtgag cgagcccgtc
    24961 gtcagcgtcg gatcgcgagc ttccgtcgcc gtgccaccgt tcgcagacgc gatcgccgat
    25021 gtgctgttct gctgatacgt gaacgtcgtc gcattggccg tcagcacggt gtacgtgccg
    25081 tcgagcgtcg agttcgaggc gccctggatc tggacgaggt cacctgccac gaacggatgc
    25141 gacgcactcg tgactgtcac gatgccggtg ttcgcgcgcg cgtagctcgt caccgcgacc
    25201 tggcggtaat cgtggccgtt gcacgtgttc ggtgcgtacg tgttcggcgc cgatgcgtat
    25261 gcccacttgt tacccttcgt tgcgagcgtc tcgccggtcc ccgggttcgt ggcaccgctg
    25321 acgagataga tccgatcgac cttcgcgccg tcttcgttca tgtagatgct gacgtagcgg
    25381 acgccctgag gcaccgagaa cgtgcccgcg tcgacccacg tccatgcgtt ggtcgtaccg
    25441 acggtaatcg tgttcgtcgg gacctgcggg ggcggcaaga gactaatacc aacgtggatg
    25501 gcatcgttcg cggtgctgct cgtgaacatc ctcacccaca cgtgatagtt gttcgtgccg
    25561 gccgtcgtga agttgaccgc gtagtccaga cgagccgcaa gtgctggagc atttgcagcg
    25621 gagacgaccg caccgatgtt cggcgtcgct tccatcgcgc tgatgccaga gaactgctga
    25681 ttcgtcgcgg taccaacggt cgtgtagtca ggtacccatg ccggtcccgc ggcaggacag
    25741 ccgtcgttga tgcgcgtgtc gccatcatca tcgacgccgt cattacagtt cacctcggca
    25801 gcaccgttgg ccgggcaacc atcgttgatc gtgccgtcac cgtcgtcgtc gactccgcct
    25861 gcggcgggtg cctcgttgca ggacgcttcc ggcgatggcc cgaatgccgg gcagccatcg
    25921 ttcaccgtgc cgtcaccgtc gttgtcgacg cggtcgtgac actgcgtctc cggtgcagta
    25981 ccgacaaacg cacaaccatc gttgatgaag ccgtcgttgt cgttgtcgac gccaacggcc
    26041 ggacatccgt cgttcacggc gttatcgccg tcgtcgtcga tgccgttctg acattgcgtg
    26101 aacgtctcgg cggtaccgac cacaggacag ccgtcgttca cggtaccgtc actgtcttcg
    26161 tctgccgtgt tgttgccgca gcttccggcg ctctcggaat ccgtgcagct tgcctctgca
    26221 gaaccactga cggcgccaac accggtactc gaatagtcct cggcctcgac gtacaggccg
    26281 gtcccgctga acgagatcgg gtaggtcgga tatccagccg cggttccgac gaccgagcat
    26341 gcatggtgcc actgcgactc gctgcatagc tgcgctccga ccgcgcgaca tgctgcgagc
    26401 gcgtcttggt acttcacgtt cgtccacggc tgcacgccgg acttcgagca gacgagcgtg
    26461 ttcttcgaac ctgcgctcga gctcgtcgaa tcgggacggg acgcctcgta cttcatcatc
    26521 tggcggccgt taccgaggtc gacccagtcc tggccgacga gcgttccatt cgctccgcct
    26581 tcgtccgtct tgccgtcgca gtcgttgtcc gtgtcgtcgc acgattcatt cgccgcgggt
    26641 gccgagcccg gcgtcgtgat cgcgcagtac gccgcaccgt tgaggttcag cgggtcgcac
    26701 tgatacgtgc cgctgccttc gcatgcgccg agctgagcga tcgccgggca gccgtcgttc
    26761 acgagaccgt cgccatcgtc gtcggttgcg ttcgcgcagt tgccgagcgt ctcgggtccc
    26821 gacgctccgc cggtgccgcc acgcgggcat ccgtcattga cgcgaccgtc gccatcgttg
    26881 tcgaccgtgt ccgcgccgca ttgcgtggac gggttttcgc ggtcctcgtg acaggactgg
    26941 cccttgttgg gctggttctc gtcgacgttg ccgtcgcagt tattgtcctt cgtgtcacag
    27001 agcgtctcgg ggaactgcac cgtgccgggg tacgtgcacg tccagccgcc ggagccgctg
    27061 cacaccgcgc tcggcgccgg accgagaggg ttcgagccgt tcggatcggt gtagccgccg
    27121 ccgcactcgc cgcccttgag gcagagcggc ggcggggtga gaccttcgtc ggtcataccg
    27181 ttgcagttgt tgtcctggcc atcgcattcc tcgacggtgc cggtcgggaa gcacggaccg
    27241 aactcgcagc cgtccgcgac gaggccgttg atgtccttgt agcccggctt gcaggcaaag
    27301 ccgcacgtgc ccacggcggg attaccttgg ggcgtgcacg tggcgttcgc gttcgcgcgc
    27361 gtcggacaga tgttgccgca ggcgccgcag tttcggacgt cggtggtctt gttgaagatc
    27421 tcgtccgtcg agccgttgca gtcgtcgtcc atgttgttgc aggtctcgaa cttcgggaag
    27481 accgcgccct gacacgtcgg ctggccaccg acgcagttga tcgtgccgaa ctcgcacgcg
    27541 ccgacgttcg tggtggggcc gcagttgccg cccgacggaa cgttgtcatc gacgacaccg
    27601 tcgcagtcgt cgtccttcgc gttgcaggct tccgcatcgt tgcggtgatc gatgaagccg
    27661 aagcagcgca ccgtgcccgt ggtgggatcg cagtggaact ggccggcgat gcactcgcct
    27721 tgatcggtgc cgcacttgcc gccggtgccc gggcaaccgt cgtcgacaac gccgtcgctg
    27781 tcgtcatcga ggccgttgcc gcactgcggg gtgccgacga gcggatccat ctcgggcgcg
    27841 ttcttcgccg gcggaggacc cgggtcgcct tcgtcgacgc cgttgccggt gccgatcgga
    27901 ccgctgcagt tgttgtcgac gccgtcgcag atctcagctt gcggctgctt gttgcacttc
    27961 agctcgccgt cgtcgcagat ctcgatgccg ccggcgcacg cacccgcgac gttgtcgcac
    28021 gggccgcctt cgtcggagac gttgtcgtcg acgagaccgt tgcagtcgtc gtctttgtcg
    28081 ttgcagagct cggggccgcc gctcgggata cagcccgcat cgacgccgcc gtcattactg
    28141 ccgttgccgt ccatgccacc gtcgccgctg ccatggccgc cgtcaccgtt ttcacagttc
    28201 aagcagtagt cgttgacgct gcacgatagc tgcgacgagc tgacgagcgc gagcgcgaga
    28261 atcatcggag agaagcgcgc gaggaatttc actgcgcacc tccttcgcca cgagccgcgc
    28321 tcgagacgcg tgccctccgc cgacgagcaa ccatcagcgc gagcgcgagc aggacgcccg
    28381 tgcttccgcc accggtcgtg tcacatccgc cgccaccacc gacggtgacg tgcgcttcac
    28441 ccgccgcgtc aggacgcagc gagtctggat cgaggcacgt accgccgcga cagacttcgt
    28501 cggcgttcgg gcacttgatg tcgttgatcg cgcacgggtc gtcttcgcac tggcccgagt
    28561 tcgggttgca ccactggccg ctgttcgtgc cgcagttctg cacgctgcac gggtcgttga
    28621 tgcacttgcc ggtgttgtca ttgcacgcct gaccaaacgg gcacggcttg ccgcacgggt
    28681 cggtctcgca catgccttgg cggcagcgtt ggccgtcggc gcactcgagg tccgcacagc
    28741 tctgcacgca cgtaccgccg aggcagtact ggttgctcgc gcacgtcaca ccctgacacg
    28801 ggttcgtgac gcacgtgccg ttcacgcacg cttgctgcgc gttacacatg ttcgggaacg
    28861 tgaggcagtt gttcggatgg cagatgccgt ccgagtcgag acagatctcc gtcgacgagc
    28921 acgtgacgcc gtcacacagg ttcacgcact ggtgctcgtt ggcgtgcgtc ggatccgggt
    28981 tcgggatgca cttctgcggg ttgccgttca ctgccgggca gctgacgcca tagcaggggt
    29041 cgttgacgca gaagaagttg tcgctcgcgc agtagtgatt cgtctggttg cagaactcgt
    29101 cggtcggaca atcaccatcg atcgtgcaag ccgtcgtgtt cgaaggctgg atgcagcgct
    29161 tgccgctggg gcatgcgaac tcggtgttcg gatcgcagcc gaacgcgcac tggcagccgg
    29221 tgcacgtcga gcccgacggg cagagctgac cctcgtcgat ctggccattg cagttgttgt
    29281 cgttgcagtc gcagacctcg gtgttgatcg gcgggtcgac aacgcacgta cgcacgccgt
    29341 tgacgcactt gttcttctcg caggtcgtac cggtgccgca cacgcagcag tcaggccccg
    29401 tgcagttcgc cgccgggttg cagacccagt tctcgtcgaa cttgccgtcg cagtcgtcgt
    29461 cgacgttgtt gcaggtgttg tcgttcgtcg ccggcgtgct gttgcacgtg atgacgccgc
    29521 cgacacactg cgtctgaccg acgccacagt tcgtcgagca gtccgcgggc gtgaagtctt
    29581 cgtcgatcgc gttatcgcag tcgtcgtcga gggcgttaca cgcgtcgctc gccgcgggct
    29641 tcacttcgcc cgagcagatg ccaaacgcgt tgctcccggc cgggcagctc gcaggcaccg
    29701 cgcaggtctt gctacccgca tggcacacgt tctgaggaat gagggccgtc gcgaccgcag
    29761 ggcagccgtc attgatacgt ccgtcggtgt cgtcgtcggc cgcgccactg cacgcgctct
    29821 ccgcgacgcc gccggcgacc gggaaggtcg ggcagccgtc gttgacgacg ccgtcgccgt
    29881 catcatctgt gttgttctgg cagcgtaggc cgctctccga gtggaggatc gggccatggc
    29941 tcgcctcgcc ggggttatcc gcgggcgggc acgagttgcc gacgctcggg acgcagttgc
    30001 ttacgacctc ggagcaaccg agcgtgaagc cgtcgcacac gccgtcgcag tcttcgtccg
    30061 caccgttgca gatttcggcc gccggaactt ggccggtgca cgtaccgagc gagccgccgg
    30121 tgcagacgcg cgtgccggga caggtaccga agctgttcgt gaccgagcag ccctgcgaga
    30181 tgccggtgca cacacaggtg ccggtgccac acgcggtgtt ggtgccgtcg cacttgacgg
    30241 agttgttgtt gctgcagcgg caggtctgat ccgcgaccgt gtcgcagtcg ttgtcgttgc
    30301 catcgcagac ttcgccggtc ggcacgcact gctggcacga caggccttcg tcgacgcgac
    30361 cgtcgcagtc ctcgtcgagt ccgttacagc cggggccttc ggtgccgcgc gggttcggaa
    30421 ccgtgttgca ctgggtgccg gtaccgtcgg ggcgacagat gctcgttccg tttcgcgcgc
    30481 acagaccgac ggccacgccg cctggcgaaa tgggatcgtt cgccggccac gtcgcctgac
    30541 acgactggtt cttgttcggg aagtcttcgt cgacgcgcac gtcgcagtcg tcgtcgcgac
    30601 cgttgcagct ctcgctgcgc accgagtcag cgaggatctg cgagatggcg agcgagaggt
    30661 cctgctcgtt ggctgcgtag taaccttcgt tcacgccggg gaggtcggca gcgccgcctg
    30721 catgcgcgat cgcttcgatc tcggcgtcgc cgggagcctt gccgaagccg atcggcttgg
    30781 tctcgatgcg atacgcgaag tccgacgccg cgtgacgcac ggtcgcgggc gtacccgacg
    30841 tcgcgccggt gttcgagccc gtgctcgcga acgtgaacgt cgtcgcgctg gtgacggagg
    30901 taatggtgaa cgtcccgttg aacgacgcgt cggtcgaacc ggagacgacg atcacgttgc
    30961 cgacggcgaa tggatgcgcg ccggtcgtcg tcaccgtcgc gacattggcg ctgcgggcga
    31021 acgtgttgac gttgacgccg tccacgcgcg gcgcggtcga gagcaggctc gcagcagcgg
    31081 gcacggtgct gttcgtgaag ttcgcgcacg tctcgtcacc gtcggtgagg aggatcgtta
    31141 cgtacgggcg gcactgcgtc gcgcagcagc agttcgcgcc gacgcacgtt ccgctgctgt
    31201 tgcaggtcgt cggattcggg ttgcacgtat cgcagcagca attcgcaccg gtgcacgcga
    31261 ccgacgggtt acacgtcgcg cgatcggagt tgcacgtcgt cggaacggtg ctcggcgcct
    31321 ggaggaacac ggcgttctgc ggatcgcggt tgatcggatc gaagccggcg agcgtctgtt
    31381 gccaaatcgt cgtcgtcccg ttcgacgcct ggaatccttg ccagtagcgc ttcgcgcctt
    31441 tgagcgcggc ggcgagcggc gtggacgtgc caccgacacc ccagatctcg gggtccgtcg
    31501 tgccggtgat cgagcacgtg ttgcacgtac cgttcgtgac gagcgcggcg gagctgttgt
    31561 tgccgtcgac gagcggcgtg aggatctcga tgccatcatc ggcggtcgta cacccgcgcg
    31621 tcgtcagaac cgtggtgccg cccgagcctt cggtgcagcg gccgaatgcg gggcaaccat
    31681 cgttgacgcg aagatcgccg tcgttgtcga tgttgtcgag gcagccgttg tacgtgatgc
    31741 cgttgacggt gaagttgcgg ttctcgtcgg tgccgctgac cgtcgcgcag ccctcgttga
    31801 cgtacccgtc gccgtcgttg tcgatgttgt cggtgcagtt gccgtcggcg ccggtgagga
    31861 tgcagctgcc gccgttgccc gtcgttccgt tcgccgagtc acagccgctg tttgccgtgc
    31921 acggagcgcc gattccgagg atgtagttgc agtcgccgca ctgcggaccg cgcgtggtgc
    31981 attgaccacc aggaccgcac gagttggtgt acgtgccact cgtcgtttcg cgaaaccgcg
    32041 cgagcgagaa caccatgtcg ccatagctgt tgacgatgtt gttgatcgcg cagaccgcgt
    32101 ggttgatctt gttatcgacc tttccgcagc tagtcggtcc cgatccggtg acgtcatcca
    32161 tcgatcctga tgtgtcgagg atcagaacga cgtacggttt tagaacgtct gcctgagcgg
    32221 tagacgtcag gacgaaaagc gtggctaccc cgaaccacgc aagcactctg cgcatggcgc
    32281 ccatcgtact catgcgccac gcgaattgga aacgctacgg tggggtaatt acgagttaat
    32341 tggcggggat cgagatcacg agaaactctc agcgaccgag cagcgaacgc cccacgacct
    32401 tgagcgcaag gatgggtttc acgccttcga agatcgggag aacctgcgcg tccgcgacat
    32461 agcggctgat cggatattcc tccgcgtagc cccagccgcc gtgcagcagc tgaccgagct
    32521 gcgtgacctc gacagcgaca tcacaggaga aaagcttcgc catcgccgcg acgggagccg
    32581 cctgacgctc gtcgccatcc atcgcgcggg cagctgcgta ggtgattgcg cgggcggcgg
    32641 tcaggcgggc catcatccgg ccgaggtggt atcgggtgag cccgtactcg ccgataggac
    32701 ggccgaactg tcggcgttct gcgacatacc gcgcggtctg ctcgatcgcg gcttgggtca
    32761 caccacaggc ccgaccgccg gtctgcaggc gtcccgcggc gaagcccgcc atctgcagcg
    32821 cgaagccgcg cccggcgccc tcctcgcctc cgacgagctg cgcgtccggc acgaaccagc
    32881 gatcgaacgc gagcgtgaac gagtgcatgc cgcgataccc gggcgtgcgg tccgctttgc
    32941 cgacgagcgt gccgccgccg tcttgcttcg cttcgaagtc gtggccgggg aagcgcggct
    33001 tgtcgacgat gaacagcgag aggccgcgcg agcctttgct catatccgga tccgtgcgcg
    33061 cgagcagtgc gattacgtcg gcgcgacctg cgaacgtgca ccacgctttc gcgccggtga
    33121 tgtgccagcc gccggtcgtc ttctccgcgc gacacgcgac gctcgcgaca tcggagccga
    33181 cgttcggctc ggtgacgctg attgccgcca tgatctcgcc cgcggcgagc ttcgggagcc
    33241 agtgctgctt ctgcgcttcg gtgccgcccg cgagcagtgc cttcgccaga atttccggac
    33301 gcgtgatcag cgagcccgct gccgcgagcg acgcgcgaga cagctcttcg gtcgtcagga
    33361 tcatggcgag attgccgagc tcgtgaccgc cgtacttctc gggaatcgag agtccgaagt
    33421 agccgagctt cgccatctcg tcgatgaagc gctccggcac gagctcgtcg tggtgatgaa
    33481 tgcgctcggc attcggcgcg acttcgcgat cggcaaacgc gcggacgttc gcgcgcactt
    33541 cggtgagcgt ttcgtcaaga ggccacgcga gccggcccgc tgctgcgatc gctgcttcgc
    33601 cgatcgcctc gacggcggac ggcgagatcg cctcgttgat cgcggcgcgc gctgcatcgt
    33661 cgaagcgcag ctcgccgagg cccagcagac cggcgaccgg ctcgagtcga ttgcgcacgc
    33721 tattcgcgag ctcacctgcg gcaacgcgcg cagcatccgc gagctcggcc ggtgtgtctg
    33781 cgagctcgct gatcacgcgc gcttcggtcg ccgcatatgc gacgcgctcg acgacgactt
    33841 gctgctcgtc gatgccttcg ccgtttcgcg tcagctggcg ggcggcctcg acggcacggt
    33901 cggcgagtga acgggcagcg acggcgatca cgttcatgcc caaaggctta gcgcgctttg
    33961 atgtcggttt gaggtctgcg tctcgtgaga tctccgatat gaatggcgct tatgaagagc
    34021 ttcctctgtc tactcctcgc gctaactcct gccgttgccc acgccgacaa gaactaccgg
    34081 agcggcaaag gcgccacctg ggattgcaag aaggatccca aggtcaacat cagccacggc
    34141 aagggcgtct acacgttcaa gggcgcctgc acgcagatca acctcaccgg cggcaagtcg
    34201 aagctgacga tcgagtcggt cgacgagctc aacatcagca gcgcggacaa caccgtcacg
    34261 atcggcacgc tgggcgcgct caacctcgtc ggcgcgaaca acaagatcac gtacaaggca
    34321 gcgaaggaag gcgacaccgc gcaggtctcg tcggtcggca cgggaaacgt cgtcgagaaa
    34381 atcggcggag ccaccgcgaa acggcaatga cgcgcactta gcgtcaagct cgtaaagtag
    34441 tcacagatag ccggcgagcg cgctcttcac ctgctcgccg tagccgccgc cgaacaagca
    34501 cgcgtgcgcg gcgagtggat acagctgatg cagcgcgatg cgctcgcgcc aaccgtcggc
    34561 gagcggccac gcttcgtcgt aggcctcgac gagcgcgtcg ggcagtccac cgaacagcgc
    34621 gagcatggcg agatcgattt cgcgatggcc gccgtacacc gcgggatcga agatgacggg
    34681 tacggcctct cgcgcggtcg gtatcgccga gacgttgccc caccacagat cgccgtgcag
    34741 gcgcgcaggc ttctccggtg gtccgaatac gtcgacgcgc gcgcgcagtt gatcgagctc
    34801 gcgatcgagg ccgagcttcg cgcgacgaca gagcggccgt agacgacgct cgacgtagaa
    34861 cgtcggccag tcgggctcgg gcttggtgtc ttgctggagc gtcgcgagat agctcggctg
    34921 gagctcgtcg atgccgaacg tcggcaggcc catgcggtgg agaccggcga gcgcgcggcc
    34981 aaatgcgatc gaatcgggac gcccccgcat gtcgagccac tcgagcgcga gccagctctc
    35041 gccttgtgca agaaccttcg gcacgcgaat cggtccgtcg cgaagccact cgaggccgaa
    35101 cgcttccgac gcgaacatcc cgggcggcgg gctgtcgtgc gacttcacga acaggcgccg
    35161 gccgtcctcg agcacgacgc gatgcgcagc gttgatatcg ccgccgctga cgctgtcgat
    35221 gctcttgatg gcgctgccga gcgccttcgc gatctccgcc ttgttcacgt cgcgaggtta
    35281 gcgcgcgcta caggcgttcg cgtacgtgat cgagcaatcc tcgacatgcg cgctcgcaga
    35341 tatcgagcac ctcttcgaag ccatcgtcct cgccgctgta cggatccgga acttcggcgt
    35401 ccaccggtgc gctcggatca aaagctcgaa gcatgcggat gctcgggagc tgcgtgcggc
    35461 cgcgcgcgag ccgctggatc gtgtcgtagt tgtcgcgatc catcgcgagg atgagatcga
    35521 agcgatcgaa gtccgctgcg accagcttgc gagcgcgatg cgtgagctcg tagccgcggc
    35581 gctgcgccgc cgcgcgagac cgcgagtcgg gcagctcgcc gacatgccac gcgccagtgc
    35641 ccgcgctgtc gatcgtcagc ttgcgatcga tgcccgcctc ttcgacgagc ttgcgaaaca
    35701 cgccctctgc cgtcggcgag cgacagatgt tgccgagaca cacgaagcaa atacgcatcg
    35761 acgtcggcga tcagctacca ggaccggagt cgagctcgcc tgtccaatac aagtagtcgc
    35821 gccacgcctc gggcacggat cgcagcgaga tccgaatcgc gaccgctggc acccaattgg
    35881 gctgcgctgg ttgcgcgagc agcttcatgc ctgcttcttg cggcgtgcga ccgcccttct
    35941 tgcggttgca gaggtagcag cacgtgacga tgttcgtcca ttccgtcatg ccgccttgcg
    36001 agcgaggcac gacgtggtcg tacgtcaggt cctggatcga tgccttgcgc gcgcagtact
    36061 ggcacttgta gttgtcgcgc gcgtagatgt tcacgcgcga aaacttcacg ggccgcgcat
    36121 gccggcggaa cgaccgcagc agacgcacga ctgccggcac cttgatgatc agggtcaccg
    36181 agtgcacgtc gcggtcgtat tcctcgagca cctcgacctt gccgaggaag agcaggctaa
    36241 tcgcccgctg ccacgagatg accttgatcg gctcgaagcc ctggttgagg agaagagtct
    36301 gagcggagtc catatgggag gggggacggc cgtgactaca gggaacctgg ccccatgctt
    36361 ccaatgtagc atcgcgttgg
//
LOCUS       DATLBZ010000413        20780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1995756, whole genome shotgun sequence.
ACCESSION   DATLBZ010000413 DATLBZ010000000
VERSION     DATLBZ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995756"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VMZ53_20975"
     CDS             <1..460
                     /locus_tag="VMZ53_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31002.1"
                     /translation="PVDASPPSPAALASPSILAALERFAEWSKANAAAPCPDAATLGV
                     IDNDPWGHPLQITCTDQPASQIAGAVSSGPDGVFGTPDDIASWELGSEITRVVRGSRW
                     PAAIATKEGIPTKKKPPADTRVRPTKTQPQTETPKASSLQVDELGLPISR"
     gene            479..2062
                     /locus_tag="VMZ53_20980"
     CDS             479..2062
                     /locus_tag="VMZ53_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31003.1"
                     /translation="MSRWWSVLAVLAFGCGGSDGDDGPNPPPAPSCAPAGGGSTTVAI
                     PTLAYTLADRYQEAWLGSPAVADLDADGVNEIIVPRHELLIVWHPDGTIAWKATLPGR
                     IWASPVVADLVPSKPGLEVAVASQGVIGAYDAQGNALPGFPFSWRNEMRSLAAGDIDG
                     DGALELVAVTSTPLEANGQRDIVMAVEMNGTAVAGFPPNTTGAAGCDTACYVTGGYDQ
                     NIALGDIDGDHVADIFATQDNAYMSLHAGTGRAFDANSIFNNRKKFSGIRWMVDYSLA
                     QQGYANNEDTEEQAHFTNTAPAIADLDGDGVGELISLASIQNAAQSDRLRGVGLYVAR
                     KDGTRPDAWVSPPRFPDYLSGLWDFGNNIVAVTNQVSVADLDPAHAGPEIVFAGFDGR
                     IHAVDARGQTLWDRTYTTTADVSTGGIAIADLSGDGSPELVFATYSADGGDLVVMDAG
                     GNILHRLPLPGRGAMPVPTIADVDRDGDLDIVVSLKDGEDRTRSVLVYSVDGSSENCL
                     SWPTGRGNYRRDGFLPPAS"
     gene            2179..2805
                     /locus_tag="VMZ53_20985"
     CDS             2179..2805
                     /locus_tag="VMZ53_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31004.1"
                     /translation="MNFGRTLSFVLAASLVPAIALADDPVDPYAPQQAPTTQQAPAPS
                     TTTTVVAPAPQPQQPVVVVNPEPRAPRTTVITRDPETTEVYDQWNAPVFATGALVFAG
                     SYGAGAIVASQSEHPGADRLYVPVVGPWLALNDWGDCPIEQPRCDKNTTDKVLLVADG
                     VFQAAGVITMVSGLLSPTHHTVTTTRTASNTKAKITPTHNGFAVVGRF"
     gene            2891..4885
                     /locus_tag="VMZ53_20990"
     CDS             2891..4885
                     /locus_tag="VMZ53_20990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31005.1"
                     /translation="MLADVSVLFLLATLVALLVAVRRLHRAQARTAKALALVQESEQF
                     FSESFHTSPMPSALVNLRTSEIAEVNSAYVRLIGRERDELIGSSVSELGLRSPSERER
                     LYRRLRRNGQMREIVTRLERRDGDVRDVVMSLDILDVAGELHAIHTVIDQTDHKRALS
                     ALRQTDERMRELAKAIDEVFYIASPDRLTMHYISPAFESIWGIPLANAYTNPLVVYDS
                     IIPEDRERLNTELARAEGSDVRVFEFRIRRADDSVRWVRLKEFPVRGREGKIERIAGV
                     ATDVSEQRVLEDQLRQAQKMESLGMLAGGVAHDFNNLLAVISSCNGLLAEVVVEKEDL
                     ELVEDISAAVLRASALTRQLLAFSRKHVAEAVILDVNAVITDTRKLLRRVVGVNIDVV
                     TSLEPELRHVRIDRGQLVQVILNLALNARDAMPAGGKLTLASRNVGDVVVLEISDNGE
                     GMPADVVARACEPFFTTKEQGKGTGMGLAVVHGIVDQSGGRIEIESKEKQGTVVRIYL
                     PGHESVSQATRPTFAAEERGVETILIVDDDDYVRRATARALRNRGYTVVEAAHGRAAL
                     SALPTANIDLMLTDIMMPGMNGRVLAEQALARFPQLRVVFMTGYTDDEVVRGGVAAGE
                     LDLIEKPFTIPALAAKVREVLDCDVPMRPTSPRIELAVAR"
     gene            4905..6236
                     /locus_tag="VMZ53_20995"
     CDS             4905..6236
                     /locus_tag="VMZ53_20995"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HUS31006.1"
                     /translation="MTRTDGQWLDAHWMPFTGNRQFKQNPRMFVSAEGSYYTDGNGKK
                     VFDGLSGLWCCGLGHGRREIADAISKTARELDYAPAFQFGHPHSFALANKIKELTPAG
                     LDYVFFTGSGSESADTSLKMARAYWRAKGQGTKRMLIGREKGYHGVNFGGISVGGIVG
                     NRKIFGQAVEADHLPHTQLALHDKSQAFTRGQPKTGAELAENLLHLIALHDASNIAAV
                     IVEPLAGSAGVIPPPVGYLQRIREICTKHDILLIFDEVITGFGRMGAYTGAEAFGVTP
                     DILNLAKQVTNGAMPLGAVVAKKDIYDTMMSVGGPEYMLEFPHGYTYSAHPVACAAGV
                     AALETLVAENGVARVRELAPYFENAVHSLKGAKHVADIRNFGLAAGLTLDAIAGEPAK
                     RPFEVAMKCLDAGFYVRYGGDTIQLAPPFIVEKSEIDKLVSVLGDALNATA"
     gene            6239..6742
                     /locus_tag="VMZ53_21000"
     CDS             6239..6742
                     /locus_tag="VMZ53_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31007.1"
                     /translation="MHEPSAHVQALLATVPRTLHRGEVARTRPGLLYMMRDWSGAWAA
                     YCKELGFPCPAALLELARAMNDVYVHCHDEHGWDLLTPDDARERRSFLARLADKHPEL
                     APRAALLPVFGQSGNLMLLARDGGVHRFTAADWQHDTMVLRDFDALLRFLVTGAIDKD
                     AAATDVL"
     gene            6800..7489
                     /locus_tag="VMZ53_21005"
     CDS             6800..7489
                     /locus_tag="VMZ53_21005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31008.1"
                     /translation="MRSLMSSGLLSVLAAAALCGSGCRRTTTDDSTVSASKQVEEAQK
                     DSKKAYERAKEAQDNAIDEQREATKAQEDVNEKRQELAEAEAKAAASAQESQAAQAEA
                     QRQGEAAQQEAQSAQQRASQLQQEQVQQQQTQQTQPASGAGSYATAQARTAETRQTQT
                     RSAQSREGAGPYSWVKPFAWNSAPKNAAPATTTDTTTTDTTTTDTTTNDTTTNTDPWA
                     DTSTNTTNGGQ"
     gene            7492..7812
                     /locus_tag="VMZ53_21010"
     CDS             7492..7812
                     /locus_tag="VMZ53_21010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31009.1"
                     /translation="MADNKNQTREHDEEPNTGMRVGGDGNKIHGDDLEDAIPGSPDGK
                     DRVRRGVETFDAEGEEVSRVGETFDIEGDIKGDMETDVGTGAESNDDLGDGGGGKRMS
                     RGRR"
     gene            complement(7884..8162)
                     /locus_tag="VMZ53_21015"
     CDS             complement(7884..8162)
                     /locus_tag="VMZ53_21015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31010.1"
                     /translation="MAISNAMELATIDFDLLSDVTGGDGWEDYKSQLSRDWQDTKSRA
                     NEAIKYNAVNGNWNAGKFADNAAGTLYNGAKMALDAIPVLGPAITNRL"
     gene            8339..8920
                     /locus_tag="VMZ53_21020"
     CDS             8339..8920
                     /locus_tag="VMZ53_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31011.1"
                     /translation="MRLAVLAVVTSMGCGRLAFDPRADGGTGDGALRDASGDASPGFI
                     AFEAESGAVVAPFRVDEDTALHPGLFFVIDGNSRGLAGVTGSVGFTVKITRAGTYHLW
                     LRSRPKDQSDDSFFVSFDGGVPQYVPTGECIYGADWHWGAWRDTTACPTLGALLGVSF
                     SVGDHTIDVSSREGESMIDRLILSDNGTFVPID"
     gene            complement(8921..10321)
                     /gene="hydA"
                     /locus_tag="VMZ53_21025"
     CDS             complement(8921..10321)
                     /gene="hydA"
                     /locus_tag="VMZ53_21025"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HUS31012.1"
                     /translation="MTRTLIKDGTVITASDTFAADVWIENGKIAGLVQPGTQLGTPDQ
                     TIDAKGRYVIPGGIDCHTHMDMPFGGTNSADDFDSGTVAAAHGGTTTIVDFAIQSKGS
                     SLRAGLDTWHAKAEGKAAIDYAFHMIMTEANQDTLEEMAAVVREGVTSFKMFMAYPGV
                     FLVDDQQIFRAMLRAGELGALITMHAEIGLPIDVLIQRALSQGHTAPIYHALTRPEIA
                     EATGTERAIALAEMAKVPVYMVHLSAQRALERVMEARDRGLPAYAETCPQYLFLDEDH
                     LRGEPGNEFKGAGYVATPALRPKHHHDHLWRGLRNYDLSVVATDHCPFCMKDQKELGR
                     NDFSKIPNGMPGVETRLHLLWEGVTQGKISMNRFVEITSTAPAKIFGMYGKKGTIAVG
                     ADADVVVWDPKREKVLGKDTLHMRVDYSPFEGRKVPGSPSAVLSRGELVVENDQWVAK
                     KKQGRGQFVRRGTFGL"
     gene            complement(10321..10632)
                     /locus_tag="VMZ53_21030"
     CDS             complement(10321..10632)
                     /locus_tag="VMZ53_21030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31013.1"
                     /translation="MLLALKATAKQLGLIAKLPAARGEGWRASIQGLTVEGHTKRVDD
                     HSLEVFHVAGPCDQAHLFAFAEHIAAIAGKQAIVGETDDDFWFVDAPAKAKTKKKKGK
                     R"
     gene            complement(10707..11093)
                     /locus_tag="VMZ53_21035"
     CDS             complement(10707..11093)
                     /locus_tag="VMZ53_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31014.1"
                     /translation="MSVAGIYPVNGGPALSDREASMLAELPETARTSRPASHAELHAA
                     VVAMAERHNYSLELEVVGTGFTAVVANKLRVVANVRGTGDETFYCQYDDESLSRQLAQ
                     ELARRCGPQIVLPSTGEDGVLVLPEA"
     gene            complement(11093..11962)
                     /locus_tag="VMZ53_21040"
     CDS             complement(11093..11962)
                     /locus_tag="VMZ53_21040"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HUS31015.1"
                     /translation="MARKVISGLIQASNAINDESKSVAEIQAAMLEKHMPMIHEAGKK
                     GVQILCLQEIFNGPYFCPGQDKRWYDAAEPVPGPTVEKLRPIAAQYKMAMVVPIYERE
                     MAGVYYNTAAVIDADGTYLGKYRKIHIPQTSGFWEKYFFRPGNLGYPTFQTRYAKIGV
                     YICYDRHFPEGARALGLNGAEIVYNPSATVAGLSEYLWKLEQPAHAVANGYYVGAINR
                     VGTEAPWNIGEFYGQSYFVNPRGQFVAMGSRDKDELIVGELDLDMIEEVRRTWQFYRD
                     RRPDSYGPLTEDR"
     gene            complement(11983..13113)
                     /gene="preA"
                     /locus_tag="VMZ53_21045"
     CDS             complement(11983..13113)
                     /gene="preA"
                     /locus_tag="VMZ53_21045"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HUS31016.1"
                     /translation="MTADLSITVNGMKFENPFLLGSGPPGTNGKVIARSYDLGWGGMV
                     CKTFSLDASKVINTAPRYAKVKARTADEVIGFQNIELISDRPYEDWLVDLKQLKKQYP
                     TKILVASIMEEYRKEAWQQIVREVQETGVDGFEMNLSCPHGLPERKMGMAMGENPDIV
                     EEVVGWVKEVARIPVWAKMTPNVGNPTVPAASALKGGADGIAMINTILSVSGIDMKTL
                     RPTPTVEGHSVPGGYSGQCVRPIALRQVMEVARANPGVQISGIGGIESGYDAAQFFLL
                     GASTVQVCTGAMLRGYEIISELKEELSKFMVDHKFTNIREFIGKSLPYFTTHHDLVER
                     QSAAREAKEAARAKSTGGKAQARDVETWKGDIAKETSTLVTG"
     gene            13208..15409
                     /locus_tag="VMZ53_21050"
     CDS             13208..15409
                     /locus_tag="VMZ53_21050"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31017.1"
                     /translation="MRWLALLAVLGGCDVIFRVDRVSFNADAAPDTPPLIEGRWTRAA
                     AGQHHTCAIDDEQHLWCWGRNYSGETGVSSSATQTDTATQVTESTWTTVSARHHTTCG
                     IKTDATLWCWGSNSTGQLGIGNATDARAPAQVMGAWREVSTGGLHTCAIKDDQSLWCW
                     GANSSGQLGDGGTVGTSSPVQVTPGTKYLHVAAGGFAPYNGAVASTCAITIDGSLWCW
                     GANDRGQLGTGVSGAIEPLPVLVGAGPWSAVAAGSTFACGITMGKLRCWGSNNFGQAG
                     DVSLASHQVPTTVMANGVDEPWEEVVAGVSHACARTTGNRMVCWGNSGAGQVMIESPA
                     VAPTEIVPPVGAGWTAIATGAAHTCGVGVDARLWCVGVNGYGELGDGGSSTVSPVHVA
                     DGYQAVAAGAVHTCALSNTNAVACAGDNSEGGLGIGNTLPKQSLTMLATGGFSSLAAG
                     VITTCGVSNNNALCWGANYRGQLGTGDLTDSAIPKQVHAGTEVSIGFEHGCTIDPSSK
                     NIVCWGYNDFGQVGNKTKAAAVKDPFTIPTQMWLHVAAGFHHTCGIDTTNVVRCWGEN
                     VFGALGTGTMNENLDPTASSLMTNAKKIYAGGYHSCAIDASDQLYCWGANYFGQLGTT
                     DGVGKPSPTQIAGSWQMMSLGMEETCGVQTDGSLWCWGYNDRGQLGIGSRARQLSPAR
                     IGTDTDWSTVAVKVYHACGTKTDGSLWCWGDNAFGELGTGNAWRTSLVQAK"
     gene            15406..17586
                     /locus_tag="VMZ53_21055"
     CDS             15406..17586
                     /locus_tag="VMZ53_21055"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31018.1"
                     /translation="MKILALLLLAGCDVLFGVDTIRTTDAGADAPIDAPRGMGWKAIA
                     TGFGHSCGIRVDDTLWCWGLNDLGELGTGDIYSSETPAQVGNATWLSIETAHQHTCGI
                     QMDHSLWCWGWNDYGSLGLGNVSQSPVPMRIGPDAKQVATGYGHTCAIAMNDSLWCWG
                     YNLYSQVGDGSTTDVVAPKRIGTDTWSRVTAGSYQTCAIRKSDQTLWCWGRNAYGSFG
                     DGSISPSTTPKLVAGETWAEISAGTEVLCGITTAGKLRCAGTSANGELGAGIVSIGAT
                     PTFQPVTIDDADLDGWTKLAIRANHGCAVRSDGALYCWGLASRLQLGSEQPDSIAARP
                     IRIAGGWREVATGTGHTCAIDASAVLSCWGADTHGQLGDGGASPKTPEKLDGAWLTIS
                     AGPFHSCALDATSKPYCSGRNSYGELGDTTKVDRQVFTPIPGASGWTTIAVGTSHTCA
                     IGNNLAYCWGSNLYGQLGDTTYTSKSSPSGVAGTYQSIATSDHSCALSGSAVACWGYN
                     LYGQLGTTNTANTNAPVTVISSNAVQLTVGVHHTCSLSSSGGAQCWGRNYEGELGNTT
                     SSNAFVPTPVTQAQSGPFETIYAGAFHTCARTLDHRLWCWGYNGYGQLGLGDLASRVS
                     PVRVSMDTWAEIATGAYHTCGLQTNGSIWCWGLNVRGQLGIGSHVAKIIPTRVGTDSD
                     WTNIISGHHHTCARKADTSWWCWGWNSDGQLGDGKAWRPAVVVP"
     gene            17583..19712
                     /locus_tag="VMZ53_21060"
     CDS             17583..19712
                     /locus_tag="VMZ53_21060"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31019.1"
                     /translation="MKRIGLLLLLAGCQQLFGLEPPAHEVDASVASGSWREIAVGAEH
                     TCAIKTDDTLWCWGGNYAGMAAAPSTMMEVTPPRQVNSERWLQLSSSYYHTCAIQADH
                     SLWCWGGNGYGVIGDGTFAAKYDPTRVTGSWTHVSAGPLHTCALRTDASLWCWGHNAT
                     GALGDGSMTDSPIPVLVAGTWKDVAAGNDHTCAIATTGALWCWGSNARLQLSLERSTP
                     VPALVDAGPWKQIAAGVDVTCGLLETGQLRCAGENSLGSLGRDTAMGNDKLGPVLLAG
                     VDRTDWTSIQLAAHTACAVRSDAAGYCWGANEFGQLASAASTSSSRPVQLGMQKWTRL
                     QTGISHTCGIDNAGNAWCVGNNSAGALGTPPTNRRSPTPIAGRWTDIAAGNSTTCAIS
                     TAGTASCAGFGISGAGGASSHQTMVQLAGSWDFIKAGFISKCGISGGELSCWGHNVAG
                     EIGDGTTNASLVPMKITAAASVSISRHTCAIDQTQKLRCWGANDSGQAGVAGGMNVRV
                     PTDIPGVWTFVSNGYSHTLGFDGLVLKSWGRGSEGQLGDGSMGSRSAPAAVGAFANSF
                     KAAYAGAYHSCALDSSFLAWCWGANSYGQLGLGDTTVRSTPTPMPARKFQQLSLGFDF
                     SCGIAEGGTLWCWGANFRGQLGVDTTLQSLTPIQVGTDTNWRAVAAGYDHACALKSDD
                     TAWCWGANNYGQLADGTAWRLTLELVP"
     gene            19759..>20780
                     /gene="mmsA"
                     /locus_tag="VMZ53_21065"
     CDS             19759..>20780
                     /gene="mmsA"
                     /locus_tag="VMZ53_21065"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HUS31020.1"
                     /translation="MATKTITHWIDGKLYEKAAERSGDVFNPATGDVQAKVAFATPSV
                     VDEAVESAWKASQTWRSVSIAKRTKILFAFRQLVDKHQHDIARLLTLEHGKVTGDALG
                     EVNRGLEVIEFACGIADLAKGEYSENVSTEVDSYSIRQPLGVVAGITPFNFPAMVPMW
                     MYPLAIACGNTFVLKPSERDPSAAMFCAQLLADAGLPPGVFNVVHGDKVAVDRLLEHP
                     KIAAVSFVGSTPIAQYIYETGTKNGKRVQALGGAKNHMIVLPDADMELAADAAVSAGY
                     GSAGERCMAISVVVAVGNAADRLIPLMKERIGKLKVGDGLESGNEMGPLINKAHRDKV
                     AGYVDAG"
BASE COUNT         3624 a         6652 c         6905 g         3599 t
ORIGIN      
        1 gcccgtcgac gcgtcgcctc cgagtccagc tgcactggcc tcgccctcga tcctcgccgc
       61 gctcgagcgg tttgcagagt ggtcgaaggc gaacgcagct gcaccgtgtc ctgatgccgc
      121 gacgctgggc gtgatcgaca acgacccgtg ggggcatccg ttgcagatca cctgcacgga
      181 ccaaccggca tctcagatcg ccggcgcagt ttcgagtggg cccgacggcg tgttcggcac
      241 gccggacgac atcgcgtcgt gggagctcgg atccgagatc acgcgcgtcg tgcgcggcag
      301 ccgatggcct gcggcgatcg ccacgaagga gggaataccg acgaagaaga agccgcccgc
      361 tgacacgcgc gtgcgtccga cgaagacgca gccgcagacc gaaactccga aagcctcttc
      421 gctccaggtc gacgagctcg gcctgccaat ctcgcgctag ccgcgatatc gtcggagcat
      481 gagccgctgg tggagcgttc tcgccgttct cgcattcgga tgtggcggca gcgatggcga
      541 cgatgggccg aacccgccgc ctgcgccatc gtgcgcgccc gccggtggtg gctcgacgac
      601 cgtcgcgata ccaacgctcg cgtatacgct tgccgatcgc tatcaagaag cgtggctcgg
      661 ctcgccggct gtcgcggatc tcgatgcaga tggcgtcaac gagatcatcg tgccgcgcca
      721 cgagctgctg atcgtgtggc atcccgatgg aacgatcgcg tggaaggcga cgttgccggg
      781 gcgcatttgg gcgtcgcctg tggtcgcgga tctcgtgccg agcaagccgg gattggaagt
      841 cgcggttgcg tcgcagggcg tgatcggcgc gtacgacgcg cagggcaatg cgttgccggg
      901 gtttccattt tcgtggcgca acgagatgcg cagcctcgcc gccggcgata tcgacggcga
      961 tggtgcgctc gagctcgtgg ccgtgacatc gacgccactc gaggcgaatg gccagcgtga
     1021 catcgtgatg gcggtcgaga tgaacggcac cgcggtggct ggctttccgc cgaacacgac
     1081 cggcgcggct ggctgcgata ccgcgtgcta cgtgaccggt ggctacgatc agaacatcgc
     1141 gctcggcgat atcgatggcg accacgtcgc cgatattttt gcgacgcaag acaacgccta
     1201 tatgtcgctg catgcgggca ccggccgcgc gttcgatgcc aattcgatct tcaacaaccg
     1261 gaagaagttc tcgggcatcc ggtggatggt cgactactcg ctcgcgcagc agggatacgc
     1321 gaacaacgag gacaccgaag agcaggcgca cttcacgaac acggcgccgg cgatcgcgga
     1381 tctcgatgga gacggcgtcg gagagctgat ctcgctcgcg tcgatccaga atgcagcgca
     1441 aagcgatcgc ctgcgcggcg tggggctcta tgtcgcacgc aaagacggca cgcgtcccga
     1501 tgcgtgggtg tcgccgccgc ggttccccga ctatctatcg ggcttgtggg acttcggaaa
     1561 caacatcgtc gcagtgacca atcaggtgtc ggtcgcggat ctcgatccgg cgcacgcggg
     1621 accggagatc gtgtttgctg gattcgacgg gcgcatccac gcagtcgacg cgcgcgggca
     1681 gacgctgtgg gatcgcacgt acacgacgac cgcagacgtc tcgacgggcg gcatcgcgat
     1741 cgcggatctc tcgggcgacg gcagtcccga gctcgtgttc gcgacgtaca gcgcggatgg
     1801 cggcgacctc gtcgtgatgg atgcaggagg caacattctg catcggctgc cgctgcccgg
     1861 gcgtggcgcg atgccggtgc cgacgatcgc ggatgtcgat cgcgatggcg atctggatat
     1921 cgtcgtgtcg ctcaaggacg gcgaagatcg aacgcgctcg gtgctcgtgt attcagtcga
     1981 cggctcgtcg gagaattgcc tatcgtggcc gacgggccga ggtaattacc gacgcgatgg
     2041 attcctgccg cccgcttcat gacggatcgt gcgtcgacgc gcgccacacc tcggtaacga
     2101 tcgctgaatg ttcgcggcct cacagatggc agcgcccttg cacttctcac attgcaaacc
     2161 ctgcaagtga ggaaacaaat gaatttcgga cgcaccctta gcttcgttct tgccgcgtct
     2221 ctcgtgcccg cgatcgcgct ggccgatgac ccggtcgatc cgtatgcccc gcagcaagcg
     2281 ccgaccacgc agcaggcgcc cgcgccgtcg acgacgacga ccgttgtcgc gccggctccg
     2341 cagccgcagc agccggttgt cgtcgtgaac ccggagccgc gtgctccgcg cacgacggtc
     2401 atcacgcgtg acccggagac gaccgaggtc tacgatcagt ggaacgcgcc tgtcttcgcg
     2461 accggtgcac tcgtgttcgc cggcagctac ggcgctggcg cgatcgtcgc gagccagagc
     2521 gagcatccgg gcgctgaccg cctctacgtg ccggtcgtcg gtccgtggct cgcgctcaac
     2581 gactggggcg actgcccgat cgaacagccg cgctgcgaca agaacacgac cgataaggtc
     2641 ctgctcgtgg cggacggcgt gttccaggcg gcgggcgtga tcacgatggt gtcgggtctg
     2701 ctctcgccga cgcaccacac ggtcaccacg acccgcacgg cgtcgaacac gaaggcgaag
     2761 atcacgccga cgcacaatgg cttcgccgtt gtcggccgct tctagctcgt agttacgcgc
     2821 acctgagcga tttcgggagc tctgaaaagg gccctcgaaa ttcgcttctt tcggccgacc
     2881 caatttatcg gtgctggccg acgtctccgt attgttcctg ctcgcgacgc tcgtcgcgtt
     2941 gctggtcgca gtgcgtaggt tgcatcgtgc gcaagcgaga acggcgaagg ccctcgcgct
     3001 cgtccaggag agcgagcagt tcttctcgga gtcgtttcac acgagcccga tgccgagcgc
     3061 gctcgtgaat ctgcgaacgt cggagatcgc agaggtcaac tccgcgtacg tgaggctgat
     3121 cggtcgcgag cgcgacgagc tgatcggctc atcggtcagc gagctcggcc tgcgatctcc
     3181 gtccgaacgc gaacggctgt atcgccgcct gcgtcgaaac ggccagatgc gcgagatcgt
     3241 cacccgactc gagcgccgcg atggtgatgt tcgcgacgtc gtgatgtctc tcgatatcct
     3301 cgatgtcgcg ggcgagctgc acgcgatcca cacggtgatc gatcagacgg atcacaagcg
     3361 cgcactttcc gcgctgcgcc agaccgatga gcggatgcgc gagctcgcga aggcgatcga
     3421 cgaggtgttc tacatcgcgt cgccggatcg cttgaccatg cactacatca gcccggcatt
     3481 cgaatcgatc tggggcatcc cgctcgcgaa tgcgtacacg aatccgctcg tcgtctacga
     3541 ctcgatcatt cccgaggatc gcgagcgcct gaacaccgag ctcgctcgtg ccgaggggag
     3601 cgacgtgcgc gtgttcgagt tccgcattcg ccgcgctgat gacagcgtgc gttgggttcg
     3661 gctgaaggag ttcccggtgc gcggccgcga aggcaagatc gagcgcatcg cgggcgtcgc
     3721 gacggatgtc agcgagcaac gcgtcctcga agaccagctg cgccaggcgc agaagatgga
     3781 atcgctcggc atgctcgccg gcggcgtcgc tcacgacttt aacaatctgc tcgccgtgat
     3841 ctcgtcgtgc aacggcctcc tcgccgaggt cgtcgtcgag aaagaggact tggagctcgt
     3901 cgaggacatc tccgctgcgg tgctccgcgc gagcgcgctc actcgccagc tcctcgcctt
     3961 ctcacgcaag cacgtggccg aagcggtcat ccttgacgtc aacgccgtca taaccgacac
     4021 gcgcaagctg ttgcgtcgcg tcgttggcgt gaacatcgac gtcgtcacgt cgctcgaacc
     4081 cgagcttcgc cacgtgcgga tcgatcgcgg tcagctcgtg caggtcatcc tcaacctcgc
     4141 gctcaacgcg cgcgatgcga tgccggccgg cggcaagctc acgctcgcgt cgcgcaacgt
     4201 cggcgacgtc gtcgtgctcg aaatctccga caacggagag ggcatgccgg ccgatgtcgt
     4261 cgcgcgcgcg tgcgagccgt tcttcacgac gaaggaacaa ggcaaaggca ccggcatggg
     4321 cctcgcggtc gtgcacggca tcgtcgatca atctggtggc cgcatcgaga tcgagagcaa
     4381 agagaagcag ggcaccgtcg ttcgcatcta cttgcccggc cacgaaagcg tgagccaagc
     4441 gacgcgaccg acgtttgccg ccgaggagcg cggcgtcgag acgatcctga tcgtcgacga
     4501 cgatgactac gtccgccgtg cgacggcgcg cgcgctgcgc aaccgcggct acaccgtcgt
     4561 ggaagctgct catggtcgcg ctgcactgag cgcgctaccg accgcgaaca tcgatctcat
     4621 gctcaccgac atcatgatgc cgggcatgaa tggccgcgtg ctcgccgagc aagcgctcgc
     4681 tcgcttcccg cagctgcgcg tcgtgttcat gaccggctac accgacgacg aagtcgtgcg
     4741 cggcggcgtc gccgcaggtg agctcgatct gatcgagaaa ccgttcacga ttcccgcgct
     4801 cgccgctaag gtgcgcgaag tgctcgattg cgacgtgcca atgcgtccta cgagcccccg
     4861 gattgagctg gcagtagcgc gctgagcggc tgtatgttcc cggcatgact cgcaccgacg
     4921 gccagtggct cgacgcgcac tggatgcctt ttacgggcaa tcgacagttc aagcaaaacc
     4981 cgcgcatgtt cgtcagcgcg gagggctctt actacacgga cggtaacggc aagaaggtct
     5041 tcgacggtct ctcgggcctt tggtgctgcg gcctcggcca tggtcgccgc gagattgcgg
     5101 acgcgatcag caagaccgcg cgcgagctcg attacgcgcc ggcgttccag ttcggtcatc
     5161 cgcactcgtt cgcgctcgcg aacaagatca aagagctcac gcccgccggt ctcgattacg
     5221 tgttcttcac cggctcgggc tcggagtccg ccgacacgtc gctcaagatg gcgcgcgcgt
     5281 attggcgtgc gaagggccag ggcacgaagc gcatgctgat cggccgcgag aagggctatc
     5341 acggcgtcaa cttcggcggc atctcggtcg gcggcatcgt cggcaatcgc aagatcttcg
     5401 gccaagcggt cgaggccgat cacctgccgc acacgcagct cgcgctgcac gacaagtcgc
     5461 aggcgttcac gcgcggccag ccgaagacgg gcgcggagct cgccgagaat ctgctgcatc
     5521 tgatcgcgct gcacgatgca tcgaacatcg ccgccgtcat cgtcgagccg ctcgcaggct
     5581 ccgccggtgt catcccgccg cccgtcggct acctacagcg cattcgcgag atctgcacga
     5641 agcacgacat cctgctgatc ttcgacgagg tcatcaccgg ctttggccgc atgggcgctt
     5701 acacgggcgc cgaagcgttc ggcgtgacgc ccgatattct caatctcgcg aagcaggtca
     5761 cgaacggcgc gatgccgctc ggtgccgtcg tcgcgaagaa agacatctac gacacgatga
     5821 tgtcggtcgg cggccccgag tacatgctcg agttcccgca cggctacacg tactcggcac
     5881 atcccgttgc gtgtgcagct ggcgtggccg cgctcgagac gctcgtcgcc gagaatggtg
     5941 tcgcgcgcgt ccgcgagctc gcgccgtact tcgagaatgc ggtgcactcg ctcaagggcg
     6001 cgaagcacgt cgcggatatt cgcaacttcg gcctcgcagc gggtctgacg ctcgacgcga
     6061 ttgctggcga gccggcgaag cgcccgttcg aggtcgcgat gaagtgcctc gacgccggct
     6121 tctacgttcg ctacggcgga gacacgatcc agctcgcgcc gccgttcatc gtcgagaaat
     6181 cagagatcga caagctcgtc tcggtcctcg gcgacgcgct caacgccacg gcgtagcgat
     6241 gcacgaaccg agcgcgcacg tccaagcgct gctcgcgacg gtgcccagaa cgctccatcg
     6301 cggcgaggtc gcgcgcacgc gtcccggcct gctgtacatg atgcgggact ggagcggcgc
     6361 gtgggcggcg tactgcaagg agctcggctt cccctgccct gctgcgctac tcgagctcgc
     6421 gcgcgccatg aacgatgtct acgtgcactg tcacgacgag cacggctggg atctactcac
     6481 gcccgacgac gcgcgcgaac gccgcagctt cctcgcgcgg ctcgccgaca aacaccccga
     6541 gctcgcgccg cgcgccgctc tactgccggt gttcggacag tcgggcaacc tgatgctgct
     6601 cgcgcgtgac ggcggcgtgc atcgcttcac ggccgccgac tggcagcacg acacgatggt
     6661 gctgcgtgac ttcgacgcgc tgcttcgctt tctcgtgaca ggcgcgatcg acaaggacgc
     6721 tgcggcaacc gacgttctct gatcgccacg ggcgcacgtg atgcatcgct ttctatgcat
     6781 cacgaaagga gaaacggaca tgcgaagctt gatgagctcg ggattgctct cagtcctcgc
     6841 tgcggccgcg ctctgcggct ctggctgcag acgaacaacg acggatgact cgacggtaag
     6901 tgcgtcgaag caggtcgagg aggcgcagaa ggattcgaag aaagcttacg agcgtgcgaa
     6961 ggaagcacag gataacgcca tcgacgagca gcgcgaggcg acgaaggcgc aggaggacgt
     7021 caacgaaaag cggcaggagc tcgctgaagc cgaggcaaag gccgcggcgt ctgcgcagga
     7081 gtcgcaggcc gcccaagcag aggcacagcg gcaaggcgaa gcggctcagc aagaggcgca
     7141 gtccgcgcag caacgcgcat cgcagctcca gcaggagcag gtgcagcagc agcagacgca
     7201 acaaacgcag ccggctagcg gcgcaggctc gtacgccacc gcgcaggcgc gcacagccga
     7261 gacacgccag acgcaaacgc gctctgcgca atctcgtgag ggcgccggcc cgtactcgtg
     7321 ggtgaagccg ttcgcgtgga acagcgcgcc gaagaacgcg gcccccgcga cgacgaccga
     7381 cacgacgacg accgacacga caacgaccga cacgacgacg aacgacacga cgacaaacac
     7441 ggacccgtgg gcggacacgt cgacgaacac gaccaacgga ggtcagtaac catggcggat
     7501 aacaagaacc agacgcgtga gcacgacgaa gagccgaaca ccgggatgcg ggtcggcggt
     7561 gacggcaaca agatccacgg cgacgatcta gaagacgcca tccccggctc tcccgacggc
     7621 aaggatcgag tccgccgcgg tgtagagacg ttcgacgccg agggcgagga ggtttcgcgc
     7681 gtcggcgaga ccttcgatat cgagggcgac atcaagggcg atatggaaac ggatgtcggc
     7741 accggcgctg aatcgaacga cgacctgggc gacggcggtg gcggcaagcg catgtcgcgc
     7801 ggtcgccgct aacgcgctcc gaaacgcaac gacgagcccg gcctatacag aggtcggact
     7861 cgttggtttg cgacagcgct cgcttacagg cggttcgtga tcgccgggcc gagcacgggg
     7921 atggcgtcga gagccatctt tgcgccgttg tagagcgtgc cggcggcgtt gtcggcgaac
     7981 ttgcccgcgt tccagttgcc gttgaccgcg ttgtacttga tggcctcgtt cgcgcgggac
     8041 ttggtgtcct gccaatcccg ggagagttgg ctcttgtagt cctcccagcc atcgccaccg
     8101 gtcacgtccg agagcaggtc gaaatcgatc gtcgccagtt ccatcgcgtt cgagattgcc
     8161 attttcattc tccttttcgt gtgagcgcac gaatgagcac acgacgtgcc ggtcgttgcg
     8221 catcgcgatc ctggagggtt ggacgaggcc tgcgcgcggc acgcgaaaac cgcggtcggc
     8281 acttcgcaga ccccaggtag ccggcgagcg atctgcgact cggttacgat ggaggtccgt
     8341 gcgattggcg gtacttgcgg tagtcacgtc gatgggttgc ggccgcctcg cgttcgaccc
     8401 gcgagccgat ggcggcaccg gcgatggcgc gctgcgcgat gcgagcggcg atgcgagccc
     8461 cggcttcatc gcgttcgagg ccgagtccgg cgcggtcgtc gccccattcc gcgtggacga
     8521 ggatacggcg ctgcatcccg gcctgttctt cgtgatcgac ggtaactcgc gcgggctcgc
     8581 aggggtgacc ggcagcgtcg gcttcacggt caagatcacg cgcgctggca cctaccatct
     8641 ctggctgcgg tcgcgaccga aggatcagtc cgacgattcg ttcttcgtga gcttcgatgg
     8701 cggggttccc cagtacgtcc cgacaggcga gtgcatctac ggcgccgact ggcactgggg
     8761 agcgtggcgc gacacgacgg cctgcccgac gctcggcgcg ctcctcggcg tgagcttcag
     8821 cgttggcgat cacacgatcg acgtgtcatc gcgcgaaggc gaatcgatga tcgatcgatt
     8881 gatcctgagc gacaacggaa cgttcgtccc gatcgactga ctacaggccg aacgtgccgc
     8941 ggcggacgaa ctggccgcga ccctgcttct tcttcgcgac ccactgatcg ttctcgacga
     9001 cgagctcgcc gcgcgacagc accgccgacg ggctgcccgg aaccttgcgg ccctcgaacg
     9061 gcgagtagtc gacgcgcatg tgcagcgtgt ccttgccgag aaccttctcg cgcttcggat
     9121 cccacacgac gacatcggca tcggcgccga ccgcgatcgt gcccttcttg ccgtacatgc
     9181 cgaagatctt cgccggcgcc gtcgacgtga tctcgacgaa gcgattcatc gagatcttgc
     9241 cctgcgtgac gccttcccag agcagatgca ggcgtgtctc gacgccgggc atgccgttcg
     9301 ggatcttcga gaagtcgtta cggccgagct ccttctgatc cttcatgcag aacgggcagt
     9361 gatccgtcgc gacgactgac aggtcgtagt tgcgcaggcc gcgccagaga tgatcgtggt
     9421 ggtgcttcgg gcggagcgcc ggcgtggcga cgtagcccgc gcccttgaac tcgttgcccg
     9481 gctcaccgcg caggtggtcc tcgtcgagaa acaggtactg cgggcaggtc tcggcgtatg
     9541 ccgggaggcc gcggtcgcgc gcttccatca cgcgctcgag ggcgcgctgc gccgacaggt
     9601 gcaccatgta gacggggact ttggccatct cggcgagagc gatcgcgcgc tcggtgccgg
     9661 tggcttcggc gatctcggga cgcgtgagcg cgtgatagat cggcgccgtg tggccctgcg
     9721 agagcgcgcg ctggatcagc acgtcgatcg gtaggccgat ttcggcgtgc atcgtgatca
     9781 gtgcgccgag ctcaccggcg cggagcatcg cgcggaagat ctgctgatcg tcgacgagga
     9841 acacgcccgg ataggccatg aacatcttga acgaggtgac gccttcgcga acgacggccg
     9901 ccatctcctc gagcgtgtcc tggttcgcct ccgtcatgat catgtggaag gcgtagtcga
     9961 tcgcggcttt gccttcggcc ttcgcgtgcc acgtgtcgag gcccgcgcgc agcgagctgc
    10021 ccttggactg gatcgcgaag tcgacgatcg tcgtcgtgcc gccatgcgcc gcagcgacgg
    10081 tgccgctgtc gaagtcatcg gcggagttcg tgccgccgaa cggcatgtcc atgtgcgtgt
    10141 ggcagtcgat gccgccggga atgacgtaac ggcctttggc gtcgatcgtc tgatccggcg
    10201 tgccgagctg cgtacccggt tgcacgaggc cggcgatctt gccgttctcg atccacacat
    10261 cggccgcgaa cgtgtcgctc gccgtgatca cggtgccgtc tttgatcagc gtgcgcgtca
    10321 tcagcgtttg cccttcttct tcttcgtttt tgctttcgcg ggtgcgtcga cgaaccagaa
    10381 gtcgtcgtcc gtctcgccga cgattgcttg cttgccggcg atcgctgcga tgtgctcggc
    10441 aaacgcgaag aggtgcgctt gatcgcaggg acccgcgacg tggaacacct cgagcgagtg
    10501 atcgtcgacg cgcttcgtgt ggccttcgac ggtcaggcct tggatcgacg cgcgccaacc
    10561 ttcaccgcgt gccgccggca gcttcgcgat gaggccgagc tgcttcgccg tcgccttgag
    10621 ggcgagcaac acctcggcat gagtcgcgta gcgcgactga ccgggtttcg tgctccccgg
    10681 tcgaatctcg taccgggcac tcatcttcac gcctccggaa gaacgaggac gccgtcctca
    10741 cccgtggagg gcagaacgat ctgtggtcca caacgacgtg cgagctcctg cgcgagctga
    10801 cgcgacagcg actcgtcgtc gtactggcaa tagaacgtct cgtcacccgt gccgcgcacg
    10861 ttcgcgacga cgcgcaactt gttcgcgacg accgccgtga agccggtgcc gacaacttcg
    10921 agctcgaggc tgtagttgtg gcgctccgcc atcgcgacga cggcagcgtg cagctcggca
    10981 tgcgatgccg gacgcgacgt gcgtgcggtc tcggggagct cagcgagcat cgacgcttcg
    11041 cgatcgctca gcgcgggtcc accattcacc gggtaaattc ctgcaacgct catcagcggt
    11101 cctccgtcag cgggccatac gaatcagggc gacgatcgcg atagaactgc cacgtgcggc
    11161 gcacttcctc gatcatgtcg aggtccagct cgccgacgat cagctcgtcc ttgtcgcggc
    11221 tacccatcgc gacgaactga ccgcgcgggt tcacgaagta cgactgaccg tagaactcgc
    11281 cgatgttcca gggcgcctcg gtaccgacac ggttgatcgc accgacgtag taaccgttcg
    11341 cgaccgcgtg cgcgggctgt tccagcttcc acaggtattc cgacagaccg gcgaccgtcg
    11401 ccgacgggtt gtagacaatc tcggcgccgt tgaggccgag cgcgcgcgca ccttccggga
    11461 agtggcggtc gtaacagatg tagacgccga tcttcgcgta gcgcgtttgg aacgtcggat
    11521 agccgaggtt gcccgggcgg aagaagtact tctcccagaa gccgctcgtc tgcgggatgt
    11581 ggatcttgcg gtacttgccg aggtacgtgc cgtcggcgtc gatcaccgcg gcggtgttgt
    11641 agtagacgcc cgccatctcg cgctcgtaga tcgggacgac catcgccatc ttgtactgcg
    11701 cggcgatcgg acgaagcttc tcgacggtgg gaccggggac cggctccgca gcgtcgtacc
    11761 agcgcttgtc ctggccgggg cagaagtacg ggccgttgaa gatttcctgc agacagagga
    11821 tctgcacgcc cttcttgccg gcctcgtgaa tcatcggcat gtgcttctcg agcattgccg
    11881 cttggatctc ggcgaccgac ttcgactcgt cgttgatcgc gttcgatgcc tgaatcaaac
    11941 cgctaatgac tttgcgcgcc atggtcaatt ctccttctcg gcctagcccg taaccagcgt
    12001 cgaggtttcc ttcgcgatgt cgcccttcca ggtttcgacg tcgcgtgcct gcgccttgcc
    12061 gcccgtcgac ttcgcgcgag cagcttcctt cgcctcgcgc gccgcggact ggcgctcgac
    12121 gagatcgtgg tgcgtcgtga agtacggcag gctcttgccg atgaactcgc ggatgttggt
    12181 gaacttgtgg tccaccatga acttgctgag ctcttctttg agctcgctga tgatctcgta
    12241 gccgcggagc atcgcgcccg tgcagacctg gaccgtcgac gcgccgagga ggaagaactg
    12301 cgcggcgtcg tagccgctct cgatgccgcc gatgccgctg atctgaacgc ccgggttcgc
    12361 acgcgcgact tccatcacct ggcgaagcgc gatcggacgc acgcactgac ccgagtagcc
    12421 gccgggaacc gagtggccct cgacggtcgg cgtcggacgc agcgtcttca tgtcgatgcc
    12481 cgacacgctg aggatcgtgt tgatcatcgc gatgccgtcg gcgccgccct tgagcgcgct
    12541 cgctgccgga accgtcgggt taccgacgtt cggcgtcatc ttcgcccaca ccggaatgcg
    12601 cgcgacttcc ttcacccaac cgacgacctc ttcgacgatg tccgggttct cgcccatcgc
    12661 catgcccatc ttgcgctcgg gcaagccgtg cgggcacgac aggttcatct cgaaaccgtc
    12721 gacgccggtc tcctggacct cgcgcacgat ctgctgccac gcttccttgc ggtactcctc
    12781 catgatggag gcgacgagga tcttcgtcgg gtactgcttc ttgagctgct tgagatcgac
    12841 gagccagtcc tcgtacggac ggtcgctgat gagctcgatg ttctggaagc cgatgacttc
    12901 gtcggccgtc cgcgccttca ctttggcgta gcgcggtgcg gtgttgatca ctttgctcgc
    12961 gtcgagcgag aaagtcttgc agaccatgcc accccagccg aggtcgtaac tgcgtgcgat
    13021 gaccttcccg ttggtgcctg gagggcccga gcccaaaagg aaggggttct cgaacttcat
    13081 tccgtttacg gtgatcgaca ggtcagcagt cacgcgcgcc tccgaggaaa gatcgggaaa
    13141 gagggtcgga tatcacaggc tccgagcggg gttcaaccgc catttgttcc gtgaaagaat
    13201 ggggcagatg aggtggctcg cccttcttgc tgttctgggc ggttgcgacg tcatctttcg
    13261 ggtcgatcgc gtctccttca acgccgacgc cgcgcccgat acgccccctc tgatcgaagg
    13321 tcgctggacc cgcgctgcgg caggtcagca ccacacgtgc gcgatcgacg atgagcagca
    13381 tctctggtgc tggggccgca actactccgg cgagacaggc gtctcgtcgt ccgcaacgca
    13441 gaccgatacc gcaacgcagg tcaccgaatc gacgtggacg accgtgagcg cgcgccatca
    13501 cacgacgtgc ggcatcaaga ccgacgcgac actgtggtgc tggggcagta actccaccgg
    13561 tcagctcggc atcggcaatg cgaccgacgc gcgcgcgccg gcgcaggtga tgggagcttg
    13621 gcgtgaggtc tcgaccggcg gtctccacac gtgcgcgatc aaggacgacc aatcgctgtg
    13681 gtgttggggc gccaattcga gcggccagct cggcgacggc ggcacggtgg gcacttcgtc
    13741 accggttcag gtcacgccgg gcacgaagta tctccacgtc gccgccggcg gcttcgctcc
    13801 gtacaacggc gcggtcgcgt cgacatgcgc gatcacgatc gatggctcgc tgtggtgctg
    13861 gggcgcgaac gatcgaggac agctcggcac gggcgtaagc ggtgcgatcg agccgctgcc
    13921 cgtcctcgtc ggtgcgggac cgtggagcgc cgtcgcagcg ggctctacat tcgcgtgcgg
    13981 catcacgatg ggcaagctcc gctgttgggg cagcaacaat tttggccaag ccggcgacgt
    14041 ctcgctcgct tcgcatcagg tgccgacaac ggtgatggcc aatggcgtcg acgaaccgtg
    14101 ggaagaggtc gtcgctggcg tgagtcacgc gtgcgcgcgc acgaccggga atcgcatggt
    14161 ttgttggggc aacagcggcg ccggtcaagt gatgatcgag agccctgcgg tcgcaccgac
    14221 cgagatcgtg cctcccgtcg gcgcaggctg gaccgcgatc gctaccggcg ctgcgcacac
    14281 gtgcggtgtc ggcgtcgatg ctcgtctgtg gtgcgtcggt gtgaatggct acggcgagct
    14341 cggcgacggc ggctcgagca cggtctcgcc cgtccacgtc gccgatggct atcaagcggt
    14401 cgcagccggc gcggttcaca cgtgcgcgct gtcgaatacg aacgcggttg catgtgccgg
    14461 cgataacagc gaaggcggcc tcggcatcgg caacacgctt cccaagcaat cgctcacgat
    14521 gctcgcaacg ggtgggttct cgagtctcgc cgcgggtgtc atcacgacct gcggtgtcag
    14581 caataacaat gcgctgtgct ggggtgcgaa ctaccgcggt cagctcggca cgggtgacct
    14641 caccgattca gcgataccaa agcaggtgca tgccgggacc gaggtgagca tcggcttcga
    14701 gcacgggtgc acgatcgatc cttcgtcgaa gaacatcgtg tgctggggct acaacgactt
    14761 cggtcaagtc ggtaacaaga ccaaggcagc ggcggtgaag gatccgttca ccatcccgac
    14821 acagatgtgg ctgcatgtcg ccgccggctt tcatcacacg tgcggcatcg acaccaccaa
    14881 cgtcgtccgc tgctggggcg agaacgtctt cggcgcgctc ggcaccggca ccatgaacga
    14941 aaacttagat cccacggcct cgtctctcat gacgaatgcg aagaagatct acgctggcgg
    15001 ttatcactcg tgcgcgatcg acgcgagcga ccagctctac tgttggggcg cgaactactt
    15061 cggtcagctc ggtacgaccg atggtgtcgg taagccgtcg cccacgcaga tcgccggctc
    15121 ttggcagatg atgtcgctcg gcatggagga gacgtgcggc gtccaaacgg acggttcgct
    15181 gtggtgctgg ggctataacg atcgcggcca gctcggcatc ggctcacgtg cgcggcaact
    15241 ctctccggcc cgcatcggca cagacaccga ttggtcgacg gtggcggtga aggtctacca
    15301 cgcgtgcgga acgaagaccg acggctcgct gtggtgctgg ggcgacaacg cattcggaga
    15361 gctcggcacc ggcaatgcct ggcgcacatc gctggtgcag gcgaaatgaa gatcctcgcg
    15421 ctgctgctgc tcgcggggtg cgacgtgctg tttggcgtcg acacgatccg aaccaccgac
    15481 gctggcgccg acgctccgat cgatgcgcca cgaggcatgg gctggaaagc gatcgcaacg
    15541 ggcttcggtc actcgtgcgg aattcgcgtc gatgacacgc tgtggtgttg gggcctcaat
    15601 gacctaggtg agctcggcac gggcgacatc tattcgagcg agacgccggc gcaggtgggc
    15661 aacgcgacgt ggctctcgat cgaaaccgcg caccagcaca cgtgcggtat ccagatggat
    15721 cactcgctgt ggtgttgggg ctggaacgac tacggcagcc tcggccttgg caacgtctcg
    15781 caatcgccag ttccgatgcg gatcggaccg gacgcaaaac aagttgcgac gggctacggt
    15841 cacacctgtg cgatcgcgat gaacgactcg ctatggtgtt ggggctacaa cctgtactcg
    15901 caggtgggcg acggctcgac gacggatgtg gtcgctccga agcgcatcgg caccgataca
    15961 tggtcgcggg tcactgccgg cagctatcaa acgtgcgcga tccggaaatc tgatcagacg
    16021 ctgtggtgtt ggggacgcaa cgcgtacggc tcgttcggcg acggctccat ctcaccgagc
    16081 acgacaccaa agctcgtggc gggcgagacg tgggcggaga tcagcgccgg aaccgaggtc
    16141 ctgtgcggca tcacgacagc cggcaagctg cgctgcgcag gcacgagtgc gaacggcgag
    16201 ctcggagccg gtatcgtctc catcggagcg acgccgacgt ttcagccggt gacgatcgac
    16261 gacgccgatc tcgatggctg gacgaagctc gcgatccggg cgaatcacgg ctgcgcggtt
    16321 cgcagcgatg gtgcgttgta ctgctggggc ctcgcatcgc gtctgcagct cggctccgaa
    16381 caaccggaca gcatcgccgc gcgtccgatc cggatcgcag gaggctggcg cgaggtcgca
    16441 accggcacgg ggcatacgtg cgcgatcgat gcgagcgcgg tcctgtcctg ctggggcgcc
    16501 gatacgcatg ggcagctcgg agacggtggc gcgagcccga agacacccga gaagctcgat
    16561 ggagcatggc tgacgatcag cgcaggcccg ttccactcct gcgccctcga cgcgacctcg
    16621 aagccgtatt gcagcggccg caactcgtac ggcgagctcg gtgacaccac gaaggtcgat
    16681 cgccaagtgt tcacgccgat tccaggagcg tccggctgga cgacgatcgc ggtcggtact
    16741 tcgcacacgt gcgcgatcgg caacaacctc gcgtattgtt ggggctcgaa cctctacggc
    16801 cagctcggcg acacgacgta cacgtcgaag agctcgccca gcggcgtcgc gggaacctac
    16861 cagtcgatcg cgacatcgga tcactcgtgc gcgctgagcg gctcggccgt tgcgtgctgg
    16921 ggctacaatc tctatggcca actcggaacg acgaacacgg cgaacaccaa cgcaccggtg
    16981 acagtcatct cgtcgaatgc ggtgcaattg acggtcggcg tgcaccacac gtgcagcctg
    17041 tcctcgagcg gtggtgcgca atgttggggc cgcaactacg aaggcgagct cggcaacacg
    17101 acctcgagca atgcgttcgt cccaacgccg gtgacccagg cgcagagcgg cccgttcgag
    17161 accatctacg ccggcgcgtt tcacacgtgc gcgcgcaccc tcgatcaccg gctgtggtgc
    17221 tggggctaca acggatatgg tcagctcggc ctcggggatc tcgcgtcgcg ggtgtcgccc
    17281 gtgcgcgtgt cgatggatac atgggcggag atcgcgaccg gcgcgtatca cacgtgcgga
    17341 ctgcagacga acggatcgat ctggtgctgg gggctgaatg tgcgcgggca gctcggcatc
    17401 ggatcgcacg tcgcgaagat catcccgacg cgcgtcggaa ccgactccga ttggacgaac
    17461 atcatctccg gccatcacca cacctgcgcg cgtaaggcgg acacttcgtg gtggtgctgg
    17521 ggctggaaca gcgacggtca gctcggcgac ggcaaagcgt ggcgacccgc agtggtggtg
    17581 ccgtgaagcg cattggcttg ctcttgctcc tcgcgggctg ccagcagctg ttcggcctcg
    17641 agccaccggc gcacgaggtc gacgcaagcg ttgcatcggg atcgtggcgc gagatcgcgg
    17701 ttggcgccga gcacacgtgc gcgatcaaga ccgacgacac gctgtggtgc tggggcggca
    17761 actacgccgg tatggccgct gccccgagca cgatgatgga agttacgccg cctcggcagg
    17821 tcaacagcga gcggtggctt cagctgtcgt cgagctatta ccacacgtgc gcgatccaag
    17881 cggatcactc actgtggtgc tggggcggca acggctatgg cgtgatcggc gacggaacgt
    17941 tcgctgcgaa gtacgacccg acgcgcgtga ccggctcttg gacgcacgtc tcggccggtc
    18001 ctctgcatac gtgcgcgctc cgcaccgacg catcgctctg gtgttggggc cacaacgcga
    18061 cgggcgcact cggcgatggc tcgatgaccg acagtccgat tccggtgctc gtcgccggaa
    18121 cgtggaagga cgtcgcggcg ggcaacgatc atacgtgcgc gatcgccacc accggcgcgc
    18181 tgtggtgttg gggctcgaac gcgcggctcc agctctcgct cgagaggtcg acgcccgtcc
    18241 ccgccctcgt cgatgcaggg ccatggaaac agattgctgc cggcgtcgat gtgacgtgcg
    18301 gcctgctcga gaccggtcag cttcgctgcg cgggcgagaa ctcgctgggt tcgctcggcc
    18361 gcgacacggc gatgggcaac gacaagctcg gtcccgtgtt gctcgccggc gtggatcgca
    18421 ccgactggac aagtatccag ctcgcagccc acacggcgtg tgcagttcgc agcgacgctg
    18481 ctggctattg ctggggcgcg aatgagtttg gccagcttgc gagcgctgcc tcgacgtcct
    18541 cgtcgcgccc ggttcagctc ggcatgcaga agtggacgcg actgcagacc ggcatcagtc
    18601 acacgtgcgg aatcgataac gcgggcaatg cgtggtgcgt cggtaacaac tcggccggcg
    18661 cgctcggaac accgccaacg aaccgtcgct cgccgacgcc gatcgcaggc aggtggacgg
    18721 acatcgccgc gggcaactcg acgacatgcg ccatctcgac cgcaggtacg gcctcctgcg
    18781 caggctttgg catcagtggc gcgggcggtg cgagctcgca ccaaacgatg gtccagctgg
    18841 ccggctcgtg ggatttcatc aaggccggct tcatctcgaa atgcgggatc tcgggcggcg
    18901 agctttcgtg ctggggacac aacgtcgcgg gcgagatcgg tgacggcacg acgaacgcgt
    18961 ctctcgtgcc gatgaagatc accgcggcgg cgtctgtttc gatctctcgc cacacctgcg
    19021 cgatcgatca gacgcaaaag ttgaggtgtt ggggagcgaa cgattccggt caagcgggtg
    19081 tcgccggagg aatgaacgtg cgcgttccga cggacatccc gggcgtgtgg acgttcgtct
    19141 cgaacggcta ctcgcatacg ctcggcttcg atggtctcgt gctgaagtcg tggggccgcg
    19201 gtagtgaggg acagctcggc gacggatcga tgggcagccg ctccgcgccg gccgcggtcg
    19261 gagcgttcgc aaactcgttc aaggccgcat atgcgggtgc gtatcactcg tgcgcgctcg
    19321 actcgagttt cctcgcctgg tgctggggcg cgaattcata cggccagctc ggcctcggcg
    19381 atacgacagt gcgctcgaca ccgacgccga tgcctgcgag aaagttccaa caactgtcgc
    19441 tcgggttcga tttctcgtgc ggcattgcag aaggcggcac gctctggtgc tggggagcga
    19501 acttccgcgg ccagctcggc gtcgacacga cgctgcaatc gttgacgcct atccaggtcg
    19561 gaaccgatac gaactggcgc gcggtggcgg ccggctatga ccatgcctgc gcgctcaaga
    19621 gtgacgacac cgcgtggtgc tggggagcta acaattacgg gcagcttgcc gacggaacgg
    19681 catggcggct gacgctcgag ctcgttccct agaaatcgat tcaccgcctc cggtagccgt
    19741 ggtacgggta ccgttcttat ggcgacgaaa acgatcacgc actggatcga cggcaagctc
    19801 tacgagaagg cagcggagcg cagcggcgat gtgttcaatc cggcgaccgg cgacgttcag
    19861 gccaaggtag cgttcgcgac gccatcggtc gtcgacgagg ccgtcgagtc ggcatggaag
    19921 gcgagccaga cgtggcgctc ggtctcgatc gccaagcgca cgaagatcct gttcgcgttc
    19981 cgtcagctcg tcgacaagca tcagcacgat atcgcgcgcc tgctcacgct cgagcacggc
    20041 aaggtcaccg gtgacgcgct cggtgaagtg aaccgtggcc tcgaggtgat cgagttcgcg
    20101 tgtggcatcg ccgacctcgc gaagggcgag tactccgaga acgtctcgac ggaagtcgat
    20161 tcgtactcga ttcgccaacc gctcggcgtc gtcgccggca tcacgccgtt caacttcccg
    20221 gcgatggtcc cgatgtggat gtacccactc gcgatcgcat gcggcaacac gttcgtgctc
    20281 aagccgagcg agcgcgatcc gtcggctgcg atgttctgcg cgcagctgct cgccgatgca
    20341 ggcctaccgc ccggcgtgtt caacgtcgtg cacggtgaca aggtcgcggt cgatcgtctg
    20401 ctcgagcacc cgaagatcgc ggccgtgtcg ttcgtcggct cgacgccgat cgcgcagtac
    20461 atctacgaga ccggcacgaa gaacggcaag cgcgtgcagg cactcggcgg cgcgaagaac
    20521 cacatgatcg ttctgcccga cgcggacatg gagctcgcgg cggacgcggc cgtgtcggcg
    20581 ggctacggct cggcgggcga gcgctgcatg gcgatctcgg tcgtcgtcgc ggtcggcaac
    20641 gctgccgaca ggctcatccc gctcatgaag gagcgcatcg gcaagctcaa ggtcggtgac
    20701 ggcctcgagt cgggcaacga gatgggcccg ctcatcaaca aggcgcatcg cgacaaggtc
    20761 gcgggctacg tcgatgcagg
//
LOCUS       DATLBZ010000414         8521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1252517, whole genome shotgun sequence.
ACCESSION   DATLBZ010000414 DATLBZ010000000
VERSION     DATLBZ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252517"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VMZ53_21070"
     CDS             <1..514
                     /locus_tag="VMZ53_21070"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS31021.1"
                     /translation="FRDDLFYRLSVMTLELPPLRSYKDNLEVLANVFLEQSATSHGKK
                     APRLGPDVLARMQAYDFPGNVRELKNTIEHAVILCTGDELAVEDLPKPLQDRAPKNRR
                     AQTQPQQTLDEAREKWLDPLERQYLTDLLAKHGGNVKKAAEAAGINTVTLYRLLKRRG
                     LHLGRTVKMG"
     gene            complement(576..1358)
                     /locus_tag="VMZ53_21075"
     CDS             complement(576..1358)
                     /locus_tag="VMZ53_21075"
                     /inference="COORDINATES: protein motif:HMM:NF019057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HUS31022.1"
                     /translation="MRNIASLITVLALSFATLSVTACATSGDEQMGDEYSDAESNASA
                     PGKLKLWQSTDGQWRFHVVSGNGRTLLTSEGYTSRTGAVNGILSVLDNGVDPAMYQVN
                     KTATGYNLRLRAANWETIAFTQVYSTKSSATRAITSCVNAVTSHLDVLEANTSGARVE
                     VLESSAGFRFNVHAQNGEVVLSSESYTTKAAAFNGAFAVQAASANATAFAIKTATDGR
                     FYFTVSADNGQIVGVSQLYTTKTAAQNGIASVQSLLRGIELL"
     gene            complement(1551..2960)
                     /locus_tag="VMZ53_21080"
     CDS             complement(1551..2960)
                     /locus_tag="VMZ53_21080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS31023.1"
                     /translation="MSYELRIGWRYLYGGRRDPLMLKLAVLFALITLAGVAQVVMSDG
                     SSPYGVIALVVGMVLTFVFTLLAFLSVFASVSVLGVALGVAALTIVLAVTSGFQQQFR
                     EKVLGVNAHVIVLKSQATFAEYRDVMKTAREIDPDVIAVQPFIFAEMLVVHIDPTGQG
                     HISGVAIKGVDPQLIRGVLDLDRHMIEGSIDTLADPTEFGQLPPIIIGKELAHKLKAK
                     VGDDVTVVVPLSNIDFDTWRAKSSAPRTRKFRVTGIFYSGFDEYDRRLMYTSLRDTQE
                     LVGRGDQVMGVELKVKDVDRAKEIAEKLERALGGPPYQVQDWYELNHNLFTALNLQKL
                     ALGIILTLIIIVAAVNMISALTMMVTDKTREVAILKSMGASSSSVSRVFQFLGIAIGG
                     VGTLIGIAVGLVTCYVVSAYGYHLDPKVYLIDRLPIDVRWYEVLMVAGATIIISALST
                     WVPAQTAASLRPIEGLRYD"
     gene            complement(3032..3259)
                     /locus_tag="VMZ53_21085"
     CDS             complement(3032..3259)
                     /locus_tag="VMZ53_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31024.1"
                     /translation="MSRRIAARTDKLGAARYIELELGSNSLLRVARPTCAEGCPLEHL
                     FDEDDEIIEILAWCRVLGDHDLSFRGTCVVV"
     gene            complement(3605..4045)
                     /locus_tag="VMZ53_21090"
     CDS             complement(3605..4045)
                     /locus_tag="VMZ53_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31025.1"
                     /translation="MKKPRTVFAAPFVIVIACGHPADRPPLAPDKPDEPDEPERIARV
                     PIDASAPVIVTDAACSAGEIACEGPGCEFTLLERTVDTTFTSHVARVDPQARELEIAI
                     PNDLRAGRAVRHASQDAGPQRSRRVRRPSRSEWSAWTSTMASRS"
     gene            complement(4042..4563)
                     /locus_tag="VMZ53_21095"
     CDS             complement(4042..4563)
                     /locus_tag="VMZ53_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31026.1"
                     /translation="MIVQKKPVDILRSLAAPRELIEWVRKMPPEEALRRAWIDVTRAD
                     WLPYLAILRGISKDAILRATCKCAGELAAPALAEPPGDRIAALLADPSTLANAEPQLE
                     DLRMQIMAHGEKPNAPAWMFWCKLALELARAIRRGNSMIGITLALRMISTAGGRRGSS
                     DLVARFRDELLLA"
     gene            complement(4574..5737)
                     /locus_tag="VMZ53_21100"
     CDS             complement(4574..5737)
                     /locus_tag="VMZ53_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31027.1"
                     /translation="MRPLLLTTLVLAACGFEHGSLTSGDAPGSGSGSGSGSGSGSGSG
                     SSSLCGWNYMPTNFDPCALPPAMPLHITATATLNIMTSQLPKLMVTQADNTPLIVVHV
                     SSLEIDAGRNWTITGPATVFAVDGDVTIKGTLTAATDVDNSTHCENGTGAPGTDSTDS
                     NGGAGGGGGGGAAANGGDGGDGAGTNHGNHGNHGAGSTGVTLSPLRGGCSGGASGKYQ
                     AAGSAAEGGRGGGGVQISTNTKIAMTFGATIDAAGRGGRGAPAAHVGAGGGGSGGGIF
                     LEGPAITLDNAILCADGGSGGEGGGASTAVGNNGNNGSCTGLIGAFTQSTNSSAGGGG
                     GNGGFKQVLGGSGAGSAASSGGGGGGGGSAGWIRIKTPGLDNNAIVTPNPMLN"
     gene            5820..6512
                     /locus_tag="VMZ53_21105"
     CDS             5820..6512
                     /locus_tag="VMZ53_21105"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS31028.1"
                     /translation="MPEAARAPRTSARRDDGTKAPRTQQQRRDETRRALLDAAVESLI
                     EVGFARTTTLEVQRRANASRGALLHHFPAKTELLVAAVDHLAEMRARELKMLAASLPD
                     ESAGRARTEAVLELLWQCFSGTFFQVAMELRTAARTDPELRPVLVAAERALRDRIVLQ
                     AKALFGKDVAEHPGLERAIDFTLQFMIGAAMTAVLHKDDGRLRDLIEDFKSLFPAVLT
                     QATTKRRKQGTR"
     gene            6509..>8521
                     /locus_tag="VMZ53_21110"
     CDS             6509..>8521
                     /locus_tag="VMZ53_21110"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS31029.1"
                     /translation="MSKDLRFDGKVAIVTGAGGGLGRSHALLLASRGAKVVVNDLGGS
                     FTGDGKSSSAADKVVAEIKEKGGEAVANYDSVTDGDKIVKTAIDTWGRIDILINNAGI
                     LRDVSFQKMTQQDWDLVYQVHVLGTMKVTQAAWNLMRDQGYGRIINTSSAAGIYGNFG
                     QANYAMAKLGIHGFTQTLAAEGKKRNIVVNTIAPIAGSRMTETVLPKELLEALKPEYV
                     SALVAKLAHESTEETGGLYEVGGGFYAKLRWERAAGKTFRLGRAITPEDIDASWKAIS
                     GFEKTEHPGSVAESMQPIMANVEAGPSKGGNQFIDVDAALGYKYPEFQSKYDERDIAL
                     YALGVGAAKDPTNEADLALVYEMSGKGMKALPSFGAVPAINMVLTMGKEGISAPGLNF
                     GLDRVLHGEQYTELKRPLPTHGKLSTKATVKNIYDKGKGAVVVTEFVTYDETGDELVR
                     NEVSTFVRGAGGWGGDRGPSAEVNVAPDRAPDKVLEDKTTENQALLYRLSGDWNPLHA
                     DPGMAKAFGFAKPILHGMCTFGIAARQVAQAFAKDGDPRYVKSIKVRFASTVLPGETL
                     VTEMWKDGNKVVFRSKVKERGEVCISNAAIELWTELPKPKPKAAPAAAGATQAAAVPN
                     SADIFRAIGGFVAGNPATAEKVKTTFLFKLNSPDSTWTIDLSTPPGA"
BASE COUNT         1552 a         2791 c         2782 g         1396 t
ORIGIN      
        1 gtttcgcgac gacctgttct atcgcctcag cgtgatgacg ctcgagctgc cgccgctgcg
       61 gagctacaag gacaacctcg aggtgctcgc gaacgtgttc ctcgagcaat ccgcgacatc
      121 acacggcaag aaggcgccgc gcctggggcc cgacgtgctc gcgcgcatgc aggcgtacga
      181 cttccccggc aacgtccgcg agctcaagaa cacgatcgag cacgcggtga tcctctgcac
      241 cggcgacgag cttgccgtcg aggatttgcc caagccgctc caagaccgcg caccgaagaa
      301 ccgccgcgcg cagacgcagc cgcagcaaac gctcgacgaa gcgcgcgaga agtggctcga
      361 ccctctcgag cgccagtacc tcaccgacct cctcgcgaag cacggcggca acgtgaagaa
      421 ggcagccgaa gccgcaggca tcaacacggt cacgctctat cgcctgctga agcgccgcgg
      481 tctgcacctc ggccgcaccg tgaagatggg ctgagccgaa actagaaaag ccccgcagac
      541 acggggcttg gttaggctag tagagacggt gtggcttaga ggagctcgat gccgcggagg
      601 agcgactgca ccgacgcgat gccgttctgc gcggccgtct tcgtcgtgta gagctgcgag
      661 acgccgacga tctggccgtt gtcagccgac accgtgaagt agaagcgacc gtccgtcgcc
      721 gtcttgatcg cgaaggccgt cgcgttggcc gacgccgctt ggactgcgaa tgcgccgttg
      781 aacgccgccg ccttcgtcgt gtagctctcc gacgagagaa cgacttcgcc gttctgcgcg
      841 tggacgttga agcggaagcc ggccgacgac tcgaggacct cgacgcgcgc gcccgacgtg
      901 ttggcctcga gcacatcgag gtggctcgtg accgcgttga cgcagctcgt gatcgcgcgg
      961 gttgccgacg acttcgtcga gtagacctgc gtgaacgcga tcgtctccca gttcgccgcg
     1021 cggaggcgga ggttgtaacc ggtcgccgtc ttgttgacct ggtacatcgc ggggtcgacg
     1081 ccgttgtcga gcaccgagag gatgccgttc accgcgccgg tgcgcgacgt gtagccttcc
     1141 gaggtcagga gcgtgcggcc attgcccgac acgacgtgga agcgccactg gccgtccgtc
     1201 gactgccaca gcttgagctt gccgggggcg ctggcgttgg actcggcgtc cgagtactcg
     1261 tcgcccatct gctcgtcacc cgaggtggcg caggcggtga cggagagggt cgcgaacgag
     1321 agagcgagaa cagtgataag cgaagcgatg ttgcgcatga tgatgggcga cccctaatac
     1381 aaggggtgga ccaatgtagg taaaagtaac tcgctggaat cacatggtgc ggatcccggt
     1441 tcagttgctt ttgtgcaact aattttgcac caaagacata tccacagccc tgaagtacgc
     1501 tttcggataa gtcgcttcag aggtctgatc tttgcgccgt tcgcgcaaat tcagtcgtag
     1561 cgcaggcctt cgatcgggcg gagcgaggcc gcggtctgcg ccggcaccca cgtcgacagt
     1621 gccgagatga tgatcgtcgc gccggcgacc atcagcacct cgtaccagcg gacgtcgatc
     1681 ggcaatcgat cgatcaagta gaccttcgga tcgaggtggt agccgtacgc gctgaccacg
     1741 tagcaagtga cgagacccac cgcgatcccg atcagcgtcc cgacgccacc gatcgcgatg
     1801 ccaaggaact ggaacacgcg cgacacgctc gacgagctcg cgcccatcga cttcaggatg
     1861 gcgacctcgc gcgtcttgtc ggtgaccatc atggtgagcg ccgagatcat gttcacggca
     1921 gccacgatga tgatgagcgt caggatgatg ccgagcgcga gcttctggag attgagcgcc
     1981 gtgaacaggt tgtgattcag ctcgtaccag tcttggacct ggtacggcgg accgccaagc
     2041 gcgcgctcga gcttctcggc gatctccttc gcgcggtcga cgtctttgac cttcagctcg
     2101 acgcccatca cttggtcgcc gcggccgacg agctcctgcg tgtcgcgcag cgaggtgtac
     2161 atgaggcggc ggtcgtactc gtcgaagccg ctgtagaaga tgcccgtcac gcggaacttg
     2221 cgcgtgcgcg gtgcgctcga cttggcgcgc cacgtgtcga agtcgatgtt cgagagcggc
     2281 acgacgaccg tgacgtcgtc gccgaccttc gccttgagct tgtgtgccag ctccttgccg
     2341 atgatgatcg gcgggagctg accgaactcc gtcggatcgg cgagcgtgtc gatcgagccc
     2401 tcgatcatgt ggcgatcgag atcgaggacg ccccggatga gctgcggatc gacgcccttg
     2461 atcgcgacgc cggagatgtg cccctgcccc gtggggtcga tatggacgac caacatctcg
     2521 gcgaagatga acggctgtac cgcgatcacg tccggatcga tctcgcgcgc cgtcttcatc
     2581 acgtcgcggt actcggcgaa cgtcgcctgc gacttcagca cgatgacatg cgcgttgacg
     2641 ccgaggacct tctcgcggaa ctgctgctga aagccggagg taacggctaa cacgatggtc
     2701 aacgcggcca cgccaagtgc gacaccgagc accgacacgc tggcgaacac actcaggaac
     2761 gcgagcaacg tgaacacgaa cgtgagcacc ataccgacga cgagcgcgat gactccgtac
     2821 gggctcgagc catcgctcat caccacctgc gcaacgccgg cgagcgtgat gagcgcgaac
     2881 aggaccgcga gcttgagcat cagcggatcg cggcgaccgc catacagata tcgccagccg
     2941 atgcggagct cgtaactcaa gggtgaccgg agcttgcctc gtcgtaggtc cacacgccag
     3001 ccgacaccgc gcggatcggt aggttgcatc cctagacaac tacgcaggtt ccgcgaaacg
     3061 ataggtcgtg gtcaccgagt acgcgacacc acgcgagtat ctcgatgatc tcgtcgtcct
     3121 cgtcgaaaag atgctcgagc gggcaaccct cggcacacgt gggccgagcg actcggagca
     3181 gcgagttgct gccgagctcg agctcgatat agcgcgccgc tccgagcttg tcagtgcgcg
     3241 ccgcgatgcg acggctcacc gcaaggaacc gttgccgctc gatcgagcac gcctggcgtt
     3301 ccagctctcg gacaccgaag cgcgcgtcct cgcagttctc gccgtgctcg agatggcacc
     3361 cgggccgcga cgcctcgcga cgtcgatcct cgaggactcc gccaccactg ctacgatcgg
     3421 tctcgtcgag agcctcgtct atcgaggcac gcatcagcgc gcactcgccg cgcaagagcg
     3481 gcgacaacgc gagtcacccg gcgttcgccg caatcccgaa tcaccccgtg ctgatggccg
     3541 tcctcgtcga ggatctggac gcgccaattc ctctcgatgc cgctatcgct gccggccggg
     3601 accgtcagga ccgcgacgcc atcgtggatg tccacgcgga ccactcgctc cgtgacgggc
     3661 ggcggacgcg gcgcgatctt tggggtccgg catcttggct ggcatgtcga acagcacgac
     3721 ctgcacgcaa gtcattcggg atcgcgatct cgagctcgcg cgcttgcgga tcgactcgtg
     3781 cgacgtgact cgtgaacgtc gtatcgacgg ttcgctcgag cagcgtgaat tcgcatcccg
     3841 ggccttcgca cgcaatctcg ccggcgctac acgccgcatc ggtaacgatg accggcgcac
     3901 tcgcgtcgat cggcacgcgc gcgattcgtt cgggctcgtc gggctcgtcg ggcttgtcgg
     3961 gtgccagcgg cggtcgatct gcgggatgac cgcatgcgat gacgatgacg aacggcgcgg
     4021 cgaagaccgt gcgcggcttc ttcatgcgag cagtagctcg tcacgaaagc gcgcgacgag
     4081 atcggagctg ccgcgacgac cgcccgccgt cgagatcatg cggagcgcga gcgtgatgcc
     4141 gatcatcgag ttgccgcgac ggatcgcgcg cgcgagctcg agcgcgagct tgcaccagaa
     4201 catccacgca ggtgcgttcg gcttctctcc gtgcgccatg atctgcattc gaagatcctc
     4261 gagctgcggc tcggcattcg cgagcgtcga aggatcggcg agcagcgccg cgatccgatc
     4321 gcccggcggt tccgcgagcg cgggtgcggc gagctcacca gcgcatttgc acgtcgcgcg
     4381 gaggatcgcg tccttcgaga tgccgcgcag gattgccaga tacggcagcc agtcggcacg
     4441 cgtgacgtcg atccacgcgc gacgcagtgc ttcttcgggc ggcatcttgc gcacccactc
     4501 gatgagctcg cgcggtgcag cgagcgaacg caggatgtcg acaggtttct tttggacgat
     4561 catcggatga gcatcagttg agcatcggat tcggggtgac gatggcgttg ttgtcgagtc
     4621 cgggtgtctt gattcggatc cagcccgcgc tgccaccgcc gccgccacca ccgcccgacg
     4681 acgcggccga accagcgcca ctgccgccca agacttgctt gaagccgccg ttgccaccgc
     4741 cgccgcctgc ggagctattc gtgctttgcg tgaatgcgcc gatgaggccg gtgcagctgc
     4801 cgttgttgcc gttgttgccg actgcggtcg acgcgccgcc accctcgccg cccgagccgc
     4861 catcggcgca gaggatcgcg ttgtcgagcg tgatcgccgg accttcgagg aagatgccgc
     4921 caccggagcc gccaccgccg gcgccgacgt gagcagcggg tgcgcctcgg ccgccgcggc
     4981 cggcagcatc gatcgtcgcg ccaaacgtca tcgcgatctt cgtgttcgtg ctgatctgca
     5041 cgccaccgcc gccgcgtcca ccttcggccg cgctgccggc cgcttgatac ttgccgcttg
     5101 caccgccgct gcagccgccg cgaagcggag agagcgtgac acccgtcgag cccgcgccgt
     5161 gattgccgtg attgccgtga ttcgttcccg cgccatcgcc gccgtcgccg ccattcgccg
     5221 ccgcgccacc accgccgcca ccacctgcgc caccgttcga atcggtcgag tccgtgccgg
     5281 gcgcgcccgt gccgttctcg cagtgcgtgc tgttgtcgac gtcggtagct gcggtgagcg
     5341 tgcctttgat cgtcacgtcg ccatcgacgg cgaacacggt ggcgggcccg gtgatcgtcc
     5401 agttgcggcc tgcatcgatc tcgagcgacg acacgtgaac gacgatgagc ggcgtgttgt
     5461 cggcctgcgt gaccatgagc ttcggcagct gcgacgtcat gatgttgagc gtcgccgtcg
     5521 cggtgatgtg cagcggcatg gccggcggta gcgcgcacgg atcgaagttc gtcggcatgt
     5581 aattccagcc gcagagcgac gagcttccgc tgccgcttcc gctgccgctt ccacttccac
     5641 ttccgctgcc gctgccgggc gcgtcaccac tcgtcagcga gccgtgctcg aacccgcacg
     5701 cggcgaggac gagggtcgtc agcaacagcg gccgcatacc caacatcata taggggttgg
     5761 cggatcgtga tacaaacagt cttgactgac tgtttcttgg gcggtacaca cctgagcgaa
     5821 tgcccgaggc tgcccgcgct ccgaggacaa gtgcccgtcg cgacgacggt acgaaggcgc
     5881 cgcgaaccca gcagcagcgc cgtgacgaaa cccgcagggc cctcctcgac gccgccgtcg
     5941 agagcctgat cgaagtcgga tttgctcgca cgacgacgct cgaggtccag cggcgggcga
     6001 atgcctcgcg cggcgcgctg ctgcaccact ttccggcgaa gacagagctg ctcgtcgcgg
     6061 cagtcgatca cctcgccgag atgcgcgcgc gggagctgaa gatgctcgcc gcgtcgctgc
     6121 ccgacgagag cgccggccgg gcgcgcaccg aagcggtgct cgagctgctc tggcagtgtt
     6181 tctccggcac gttcttccag gtcgcgatgg agctgcgcac ggcggcccgc accgatccgg
     6241 agttgcgccc cgtgctcgtc gcggcagagc gcgcgcttcg cgaccgcatc gtgctgcagg
     6301 caaaggcgct gttcggcaag gatgtcgccg aacatcccgg cctcgagcgc gcgatcgatt
     6361 tcacgctcca gttcatgatc ggcgccgcga tgacggcggt gctgcacaaa gacgacggtc
     6421 gcttgcgcga cctcatcgaa gatttcaaat cgctgtttcc agcggtgttg acccaagcaa
     6481 caaccaagcg acgcaaacag gggacgagat gagcaaagat ctgcggttcg acggaaaagt
     6541 agcaatcgtg accggcgcag gcggaggcct gggtcgttcg cacgccctcc tgctcgcgtc
     6601 gcgaggcgcg aaggtggtcg tcaacgacct cggcggcagc ttcacgggag atggcaagtc
     6661 ctcgtcggcg gctgacaaag tcgtcgccga gatcaaagag aagggcggcg aggcggttgc
     6721 caactacgac tcggtcaccg acggcgacaa gatcgtcaag acggcgatcg acacgtgggg
     6781 caggatcgac atcctcatca acaacgccgg catccttcgc gatgtctcgt tccagaagat
     6841 gacgcagcag gattgggacc tcgtctatca ggtccacgtc ctcggcacga tgaaggtcac
     6901 gcaagccgcg tggaacctca tgcgcgatca gggctacggc cgcatcatca acacgtcgag
     6961 cgccgccggc atctacggca acttcggcca agcgaactac gcgatggcca agctcggcat
     7021 ccacggcttc acgcagacgc tcgcggctga aggcaagaag cgcaacatcg tcgtcaacac
     7081 gatcgcgccg atcgccggct cgcgcatgac cgagacggtt ctgccgaagg agctcctcga
     7141 agcgctcaag cccgagtacg tctcggcgct cgtcgcgaag ctcgcgcacg aatcgacgga
     7201 agagaccggc ggcctctatg aagtcggcgg cggcttctac gcgaagctgc gttgggaacg
     7261 cgccgcgggc aagacgttcc gcctcggtcg cgcgatcacg cccgaggaca tcgacgcctc
     7321 gtggaaggca atcagcggct tcgagaagac ggagcatccg ggctcggtcg ccgagtcaat
     7381 gcagccgatc atggcgaacg tcgaagcagg cccgagcaag ggcggcaatc agttcatcga
     7441 tgtcgacgct gcgctgggct acaagtatcc cgagttccag tccaagtacg acgagcgtga
     7501 tatcgcgctc tacgcgctcg gcgtcggcgc cgcgaaggat ccgacgaacg aagccgacct
     7561 cgcactcgtc tacgagatga gcggcaaggg catgaaggcg ctgccgagct ttggcgcagt
     7621 gcctgcgatc aacatggtcc tgacgatggg caaagagggc atcagcgccc cgggcctgaa
     7681 cttcggcctc gatcgcgtcc ttcacggcga gcagtacacg gagctgaagc gcccgctgcc
     7741 gacacacggc aagctgtcga cgaaggcgac cgtcaagaac atctacgaca agggcaaggg
     7801 cgctgtcgtc gtcaccgagt tcgtcacgta cgacgagacc ggcgacgagc tcgtgcgcaa
     7861 cgaagtctcg acgttcgtcc gcggtgcggg cggttggggc ggcgatcgtg gtccgtcggc
     7921 agaggtcaac gtcgcgcccg atcgcgcgcc ggacaaagtc ctcgaggaca agacgaccga
     7981 gaatcaagcc ctgctctatc gcctctccgg cgattggaac ccgctgcatg cggatccggg
     8041 catggcgaag gcgttcggct tcgcgaagcc gatcctccac ggcatgtgca cgttcggcat
     8101 cgccgcgcgc caggtcgcgc aggcgttcgc gaaggacggc gacccgcgct acgtgaagtc
     8161 gatcaaggtt cgcttcgcgt cgacggtgct ccccggcgag acgctcgtca ccgagatgtg
     8221 gaaggacggc aacaaagtcg tcttccgctc gaaggtcaaa gagcgcggcg aggtgtgcat
     8281 cagcaacgct gcgatcgagc tgtggaccga gctgccgaag ccgaagccga aagctgctcc
     8341 tgcagcggca ggcgcgacgc aggcggcagc ggttccgaat agcgcggaca tcttccgtgc
     8401 gatcggcggc ttcgttgcgg gcaaccccgc gacggccgag aaggtgaaga ccacgttcct
     8461 gttcaagctg aacagcccgg actcgacgtg gacgatcgat ctgtcgacgc cgccgggcgc
     8521 t
//
LOCUS       DATLBZ010000415         4287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8451921, whole genome shotgun sequence.
ACCESSION   DATLBZ010000415 DATLBZ010000000
VERSION     DATLBZ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8451921"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VMZ53_21115"
     CDS             <1..507
                     /locus_tag="VMZ53_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31030.1"
                     /translation="QVSPQQMKTELLAQIADARHHLEQYPASCSARERREQAFRTLYG
                     IAYELNTTQAAIDAEYRTDADYVLGELEYTKSKTCCWNSSTSAMYDIIQKKAAADKAA
                     ADARNVCEPPVVFKSRSDGYKYWADYAASIGRGAEWRAWSEDETCAQKNVASDTETAL
                     SATPYCSL"
     gene            521..1879
                     /locus_tag="VMZ53_21120"
     CDS             521..1879
                     /locus_tag="VMZ53_21120"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HUS31031.1"
                     /translation="MIVASGLVELIGKTPLVRVSCFDTAGSELWLKLESHNPGGSIKD
                     RIGLSMITAAEADGRLQPGGHIIEATAGNTGLGLALVAALRGYRLTLVVPDKMSQEKV
                     THLKALGAEVIWTRSDVGKGHPAYYQDMAQRIAGETGAYYVNQFENAANPAAHESTTA
                     PEIWEQTGRQLDAVVCGVGSGGTITGMSRFFGEAAPEVEIVLADPKGSVLAGYVATGE
                     VGVAGSWLVEGIGEDFVPPICDLSRVRRAYTIDDAEAFSTVRTLLAKEGILAGTSSGV
                     LIAAALKYCRDRQAPCKVVTFVCDSGAKYLSKAYRDEWLADQGLLARTEYGDLRDVVA
                     RRGGQLIDVSPDDTLRVTYARMRLHDVSQLPVLDRGALVGIIDESDLLVAELAGGKPF
                     DRPVKDVMSTKLETLTPSATIEDLVAVLDRGLVGVIVDKGALFGIVTRIDLVNYLRRR
                     IA"
     gene            1876..3474
                     /locus_tag="VMZ53_21125"
     CDS             1876..3474
                     /locus_tag="VMZ53_21125"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HUS31032.1"
                     /translation="MSSTMMDIPLTLDWIADRAERWNGNVEVVSRRPDKSLTRSTYAQ
                     VIGSARRLARGLLAAGIKKGDRVATLMWNHAEHLEVYFGIPLSGAAIHTLNLRLHPDE
                     IAFIANDARDRIVIVDDVLLPLLDKVIAAGGKFERVLVVGNAGERESYAQFLETAPSQ
                     GALPSLAESDELGVCYTSGTTGRPKGVVYSHRSTLLHSLVATMPDALDLSRRDTLLPV
                     VPMFHVNAWGLPYMAAMTGTKLVCPGPHLDPASLLDLMERERVTVAAGVPTIWIGIRD
                     VLDANPGKYKLQPNVRMIVGGSAAPEQLIRDFDRHGLSLIHAWGMTETSPIGTVSRLL
                     PEHDATDDATRYALRARQGRPVPLVDIRVRNEQGDVPTDDSTSGEVLIRGPWIAASYS
                     GGHTPERWTADGYFRTGDVARVDASGFIKLTDRIADLIKSGGEWIASQELENALMGHP
                     AVREAAVIGVAHPKWSERPLAAVVLKDGQSATADDLRAFLAPRFPKFWLPDAFDFVDA
                     IPRTSAGKFKKTELRDKYRDYKWV"
     gene            3549..4190
                     /locus_tag="VMZ53_21130"
     CDS             3549..4190
                     /locus_tag="VMZ53_21130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUS31033.1"
                     /translation="MVTSLRATVKNAVIDRIPGVLRRGPATVKRIALTFDDGPDEQTP
                     ALLELLDDLGVPATFFLCGVHATEHPDLVREYLRRGHQIANHGYDHTRFSKLGRKALL
                     DQLSRTDVAIGGQATGKLWVRPPHGAFDTTSLISMRTAGYTVAMWSVDSLDHTDNDPA
                     SLAERCGPANVGPGDVLLFHEGQPWTREALPRIVASLHASGLECVTMHDLFAR"
BASE COUNT          732 a         1442 c         1407 g          706 t
ORIGIN      
        1 caagtctcgc cgcagcagat gaagacggag ctgctcgcgc agatcgccga tgcgcgccat
       61 cacctcgagc aatatcccgc gtcgtgctcg gcgcgcgagc gtcgcgagca agcgttccgc
      121 acgctgtacg gcatcgcgta cgagctcaac accacgcagg cagcgatcga cgccgagtac
      181 cgcaccgacg cggactacgt gctcggcgag ctcgagtaca cgaagagcaa gacgtgctgc
      241 tggaacagct cgacgtcggc gatgtacgac atcatccaga agaaggccgc tgccgataag
      301 gccgctgccg atgcgcgcaa cgtgtgcgag ccgcctgtcg tcttcaagtc gcgcagcgac
      361 ggctacaagt actgggcgga ctacgccgcc tcgatcggcc gcggcgcgga gtggcgtgcg
      421 tggagcgagg acgagacctg cgcgcagaag aacgtcgcga gcgacacgga aaccgcgctg
      481 tcggcgacgc cctactgctc gctctgatat cgtccgcggc gtgatcgtcg cttctggcct
      541 cgtcgagctg attggcaaga caccgctcgt gcgcgtgtcg tgcttcgaca ccgcaggcag
      601 cgagttgtgg ctcaagctcg agagtcacaa cccgggcggc tcgatcaaag atcgaatcgg
      661 cctctcgatg atcacggcag cggaggcgga cggccggctt cagccaggcg gccacatcat
      721 cgaagcgacg gcaggcaaca cgggcctcgg tctcgcgctc gtcgccgcac tgcgcggcta
      781 tcgcttgacg ctcgtcgtgc ccgacaagat gagccaagag aaggtcacgc acctcaaggc
      841 gctcggcgcc gaagtgatct ggacgcgcag cgacgtcggc aagggccacc ccgcctacta
      901 ccaagacatg gcgcagcgga tcgcgggcga gaccggcgcg tactacgtca accaattcga
      961 gaacgccgcg aaccccgccg cgcacgagag cacgacagcg cccgagatct gggagcagac
     1021 cggacgccag ctcgatgccg tcgtgtgcgg cgtcggcagc ggcggaacga tcaccggcat
     1081 gtcgcgcttc ttcggtgagg ccgcgccgga agtcgaaatc gtgctcgctg atccgaaggg
     1141 ctcggtgctc gctggctacg tcgcgaccgg cgaggtcggc gtggcgggca gctggctcgt
     1201 cgagggaatc ggcgaggact tcgtgccgcc catctgcgat ctctcgcgcg tgcgtcgcgc
     1261 gtacacgatc gacgacgcgg aagcattctc gacggtgcgc acgctgctcg cgaaggaagg
     1321 catcctcgcc ggcacgtcga gcggcgtgct gatcgccgcg gcgttgaagt actgccgcga
     1381 tcggcaagcg ccgtgcaagg tcgtcacgtt cgtgtgcgac agcggcgcga agtatctgtc
     1441 gaaggcgtat cgcgacgaat ggctcgctga tcaggggctg cttgcgcgga ccgagtacgg
     1501 agacctgcgc gatgttgtgg cgcggcgtgg agggcagctg atcgacgtct cgcccgacga
     1561 cacgctgcgc gtcacatacg cgcgaatgcg cctgcacgat gtctcgcagc ttcccgtgtt
     1621 ggatcgcggc gcgctcgtgg ggatcatcga cgagagtgac ttgctcgtcg cagagctcgc
     1681 gggtggcaag ccgttcgaca ggcccgtcaa agacgtgatg tcgacgaagc tcgagacgct
     1741 gacgccgtcg gcgacgatcg aagatctggt ggcggtgctc gaccgtggcc tcgtcggcgt
     1801 gatcgtcgac aagggcgcgt tgttcggaat cgtgacgagg atcgacttgg tgaactatct
     1861 gcggaggagg atcgcgtgag ctcgacgatg atggacatcc cactgacgct cgactggatc
     1921 gcggaccgcg cggagcgctg gaacggcaac gtcgaagtgg tgtcgcgccg tccggacaag
     1981 tcgctcacgc gctcgaccta cgcgcaggtc atcggaagtg cacggcgctt ggcgcgaggc
     2041 ctcctcgctg ccggcatcaa gaagggcgac cgcgtcgcga cgctgatgtg gaaccatgcc
     2101 gagcacctcg aggtgtactt cggcatcccg ctgtcgggcg ccgcgattca cacgctgaac
     2161 cttcgcctgc atcccgacga gatcgctttc atcgcgaatg acgcgcgcga tcgcatcgtc
     2221 atcgtcgacg acgtcctgct gccgctcctc gacaaggtga tcgccgcggg cggcaagttc
     2281 gagcgcgtgc tcgttgtcgg caatgcaggc gagcgagagt cgtacgcgca gtttctcgag
     2341 actgcgccct cgcaaggcgc cctgccctcg ctcgccgaat ccgacgagct cggcgtctgc
     2401 tacacgagcg gaacgacggg gcggccgaag ggcgtcgtct actcgcatcg ctcgacgctg
     2461 ctgcattcgc tcgtcgccac gatgcccgac gcgctcgatc tctcgcgtcg cgacacgctg
     2521 ctccccgtcg tgccgatgtt ccacgtcaac gcgtggggac tgccgtacat ggcggccatg
     2581 acgggcacga agctcgtgtg tccggggccg catctcgatc ccgcgtcgct gctcgatctc
     2641 atggagcgcg agcgcgtcac cgtcgcggcc ggcgtgccga cgatctggat cggcattcgc
     2701 gacgtcctcg atgcgaaccc gggcaagtac aagctgcagc cgaacgtgcg gatgatcgtc
     2761 ggtggctccg cggcgcccga acagctcatc cgcgacttcg atcgccacgg tctatccctg
     2821 attcacgcat ggggcatgac cgaaacgtcg ccgatcggca cggtgtcgcg gctgctgccc
     2881 gagcacgacg ccaccgacga cgctacgcgc tacgcactgc gcgctcgcca gggtcgcccc
     2941 gtgccgctcg tcgatatccg cgtccgcaac gagcaaggcg acgtccccac cgacgacagc
     3001 acgagtggcg aagtcctcat ccgcggcccg tggatcgcgg cctcgtactc cggcggccac
     3061 actcccgagc gctggaccgc cgatggctac ttccgcaccg gcgacgtcgc ccgcgtcgat
     3121 gcctccggct tcatcaagct caccgatcgc atcgcggacc tcatcaaatc gggcggcgag
     3181 tggatcgcgt cgcaagagct cgaaaacgcg ctgatgggtc accccgccgt tcgcgaagct
     3241 gccgtcatcg gagtcgcgca tcccaaatgg agtgagcgcc cgctcgccgc cgtggtcctc
     3301 aaagacgggc agtccgccac cgccgacgat ctgcgcgcgt tcctcgcgcc gcgctttccc
     3361 aagttctggc ttcccgatgc gttcgacttc gtcgacgcga ttccacgcac gtcggcgggc
     3421 aagttcaaga agaccgagct gcgcgacaag taccgcgact acaagtgggt gtgagcaatc
     3481 cgtgaggcgg ccgagcgctg cctcgtgagc ggcttgtaga tgccaggaaa acccgctacc
     3541 cattccaggt ggtcacctct cttcgcgcga cggtcaagaa cgcggttatc gaccgcattc
     3601 ccggtgtgct gcgtcgtggt ccggccacgg tcaagcgcat cgcgctgacg ttcgacgacg
     3661 gccccgacga gcaaacgccc gcgctcctcg agctcctcga cgatctgggc gtgccggcga
     3721 cgttctttct gtgcggcgta cacgcgaccg aacatccgga tctcgtccgc gaatacctcc
     3781 ggcgtggtca ccaaatcgcg aatcacggct acgaccacac gcgcttctcg aagctcggtc
     3841 gcaaagcgct gctcgatcag ctctcgcgca ccgacgttgc gatcggcggt caagcaacgg
     3901 gcaagctgtg ggtgcgcccg ccccacggcg cattcgacac gacgtcgctc atctcgatgc
     3961 ggacggctgg ctacaccgtc gcgatgtggt cggtcgattc gctcgatcac accgataacg
     4021 atccggcgtc gctcgccgaa cgctgcggcc ccgccaacgt cggcccgggc gatgtgctgc
     4081 tcttccacga gggccagccg tggacgcgtg aagcgctgcc tcgcattgtc gcgtcgctgc
     4141 acgcctccgg cctcgagtgc gtgacgatgc acgacctgtt cgcgcgctag cctgcgaacg
     4201 ttttggcgcc ggcgcgcaac cgccgccgct cctttgttat cgtcgtcgaa tgaagtcggt
     4261 gatgtttgga gtggtatgcg tgctggc
//
LOCUS       DATLBZ010000416        11814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4211350, whole genome shotgun sequence.
ACCESSION   DATLBZ010000416 DATLBZ010000000
VERSION     DATLBZ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4211350"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /gene="lon"
                     /locus_tag="VMZ53_21135"
     CDS             <1..2099
                     /gene="lon"
                     /locus_tag="VMZ53_21135"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HUS31034.1"
                     /translation="APVSRATPAELQATTTKVRDYLLAVVTDAQAKEAKGEPSKEQSK
                     EPRSELTRAQVQAIVDPDKLVDAAVPYLELERDDLAQLLVETDTMKRLERIIPSLERQ
                     ATVLRLKADIGAELEGESSRNHRERVLRDRMRQIQEELGEQDDNAEIDELRKKIEDSK
                     MSDEVRAVAKKQLSRMSQMASSSPEYNIARTYVENLLEIPWNVTTDDRLDVAAARAIL
                     EAEHSGLEKVKRRILEFLAVRKLAPNKHGPILLLVGPPGVGKTSLGRSIASSLGRKYV
                     RISLGGVRDEAEVRGHRRTYIGALPGRIVAGLKKAGSMNPVFVLDEIDKLAADMRGDP
                     AAAMLEVLDPEQNKDFVDHYVEVPVDLSKVMFICTANQLDTISQPLLDRMETVELSGY
                     TTVEKLAIAKNHLLPKQLGEHGISKDQMQVDDDALEDIIHSYTREAGVRNLEREIAAV
                     CRGAAVKVAEGVTGVHVTKEMLQELLGPPRFVSDTAERKPEVGVITGLAWTPVGGDIM
                     FIEARRYPGKGDVRLTGQMGDVMKESATAAVSWTRANAARLGIDSDKIANSDLHIHLP
                     QGAIKKDGPSAGVALTCAVVSVFTGRPIRNDVAITGEIDLRGHALPIGGVKEKVLAAH
                     RAGIKIVFMPERNQKDTLDIPEEIRNQLDLRFMKKIDDALEVALGPMPPASEPEPAIP
                     PPTAPSRGSMGDRLPS"
     gene            2099..2605
                     /locus_tag="VMZ53_21140"
     CDS             2099..2605
                     /locus_tag="VMZ53_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31035.1"
                     /translation="MARAALLFLAVAACSGSGRGHKPPSEAECQAYRDKLFAYMPEAD
                     RTKVTAMGLGKQTPIELALCQQRMKSDEIACVVASKTFDEALLCKPAVDDRPAEMKRS
                     PAECQAFMEHVTKLANDAAQGDASGPPFTPFMAKLTARECGRWLSKERYECVMQAPTT
                     LGIVGCPP"
     gene            complement(2602..4446)
                     /locus_tag="VMZ53_21145"
     CDS             complement(2602..4446)
                     /locus_tag="VMZ53_21145"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HUS31036.1"
                     /translation="MRWTLGAALFAAVGCYDPQVPLGIPCSDTGECPRGQECDTISNT
                     CGEPAELRTWRDDSAADFSQTGAVLIDSAVEKAGFVGPVPYATGRAKVSGVVGNKIGA
                     DAAAATWANVDVARAGTSFATALALNYDILVPRGVGLTQADDVTVLVECEIQLDAAGA
                     WRFELDANDNGFLELAPPGGDFTRVVNSANAATSATYNVATPGWYRLRAAFADAAMFM
                     DFHVRMDSPAIQGGFREIAPDQMRVRVDGIDGAIFDGFDDTYLLRYFGSTLLATPIQL
                     TLPDDPYAINIGILSLSMRAVGQFLIDKEGDYSFTVSSHHGHRVWIDGQQVANAFGGT
                     DATTNVAPIHLDAGWHYLVFDAMKANSSALLTASIVVASGPDLVGQSFPVDHLRPVPS
                     NAFRWAADSDAASTTITDGGSIMRPLTLLLPNGAMAKSIDADFEFNHTAQAQVGITLD
                     PPAGSNITLVATGALAGSGQHVEHVTVPAADAGASWNLIASDSTVDAITGTLDLFSVT
                     MLYDGGLKPFPTTYRYESAPKDLGNVVALGPLSWQTRQGSAAKVQFRMCADASCAGEA
                     WTDVPQSGAVPSVALKPYAQYAVEFTGDGVIPTALDWIELQYSSRPPI"
     gene            complement(4465..5697)
                     /locus_tag="VMZ53_21150"
     CDS             complement(4465..5697)
                     /locus_tag="VMZ53_21150"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HUS31037.1"
                     /translation="MRLALVTLLFATACRGDGRAPSRGPEPKPTQLGGSGSASVAPAT
                     TPPPGTDELVAPPAEIAAKVALKEIAHGLKRPVLLTSAPNDPRRRLFIVEQPGAIKIL
                     EGGKVSDKAFFTIDGLSHGNEEGLLGLAFHPRFAETKKLYVNYTAKDMSTHIVEYKVS
                     ASDPDVVDTASRREIIRIEQPYSNHNGGHLAFGPDGKLYAGMGDGGAANDPHGNGQNE
                     KALLAKMLRFDVDGDAKPEIVHIGLRNPWRFSFDSKTGDLFIGDVGQNLFEYVHAVAG
                     TDTSRKNFGWNIVEGTHCFDPDSGTSKQTCDRTGLTPPLVEYPHEQGCSITGGYVYRG
                     KALPALAGRYFYADYCTGLLRSFVWVSGAVREHWDWKPAIDKQGVITQVSSFGVDHDG
                     ELYIVELTGSIYQLVPSS"
     gene            5916..6257
                     /locus_tag="VMZ53_21155"
     CDS             5916..6257
                     /locus_tag="VMZ53_21155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31038.1"
                     /translation="MQVNAHIARKNTPVPMIAGHANAKNSKDLQRYFEYSASGVDVVE
                     DGLKEITFGEFLVEQQVLDRFQLFRALQMQDRLPGVRLGECAAALGYAPINAIERLYE
                     RFAQLSTVDVP"
     gene            complement(6258..6620)
                     /locus_tag="VMZ53_21160"
     CDS             complement(6258..6620)
                     /locus_tag="VMZ53_21160"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS31039.1"
                     /translation="MSTKETVLAYLHAIESHNLDLVAQFLHDDVQVTEHPNKLNPAGK
                     TYDKAALREAGERGKALLASERYEVRAMTVEGDHCVAQILWTGVLADGREMKAHICSA
                     IELRDGRVFRQEQYDCFI"
     gene            complement(6636..7688)
                     /locus_tag="VMZ53_21165"
     CDS             complement(6636..7688)
                     /locus_tag="VMZ53_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HUS31040.1"
                     /translation="MKTVTLIEGDGIGPEIAAATVRAIEAAGGKIAWERADAGAIAVE
                     RHKDPLPPATIESIKKNGIALKSPLATPSGGGYRSVNVTLRQHFDLYANVRPVETIAG
                     VPSRYTGVNLVIVRENTEDLYAGVEHYVDPRRTAAESIAIITKFGSERVIVYAFEYAR
                     RMGRKKVTLVHKANILKMSNGLFLDTGKEVAKRYPEIQFDDMIVDATAMKLVLQPERF
                     DVIVTMNLFGDILSDLTAGLVGGLGVAPAANIGDGGIAMFEAVHGTAPDIAGKGIANP
                     TGLMLSAALMLDHLEQRDAANRLRRGIKAALAKAETRTGDLGGKANTMQYTEAVIRAM
                     DMSPDASGTMVIRGVD"
     gene            complement(7689..8015)
                     /locus_tag="VMZ53_21170"
     CDS             complement(7689..8015)
                     /locus_tag="VMZ53_21170"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUS31041.1"
                     /translation="MAWHAVAKVGQLQAGDVIGLEVEGVSMIVGRDGERYFAMQRQCV
                     HQGGDLADGIVSRGHVICANHGWRFSTATGRHDSSSDACLVTYAVRVNGETIEVDPVP
                     RTLRGY"
     gene            complement(8018..8362)
                     /locus_tag="VMZ53_21175"
     CDS             complement(8018..8362)
                     /locus_tag="VMZ53_21175"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS31042.1"
                     /translation="MPTHHTVDFFEIPVLDMAGSKAFYGAAFGWSYKDYGPDYADVQG
                     AGMTGGLRLVKAPPTSGGPLLIVYSDDLEASERAVTAAGATITERHEFPGGRRFHFVD
                     PGGCELAVWTKA"
     gene            complement(8437..9918)
                     /locus_tag="VMZ53_21180"
     CDS             complement(8437..9918)
                     /locus_tag="VMZ53_21180"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS31043.1"
                     /translation="MAETALRVDGVRHVLVADADPAGVAHAREALAGVDVEEVGGAPA
                     MLERLVGSPFDLVLLDPALGDDEDGALALLHRARTVAPDTVVVIWSAKPTVEFTVRVM
                     RSGALDVLHKEAATAEIRSVVDRAIQHGALAREVRRLRGEVEKARGLGEVIGESPLMR
                     QLLQVIERVAASDATVLIVGESGTGKELLARTIHRVGPRKDGPFVAFDCSALAPSLIE
                     AELFGHEKGAFTGAMRARRGLFREANGGTIFLDEIGDIDASVQNKLLRVLQEREIKPV
                     GGDRPVQIDVRVVAATNKDLKNLVGRGQFREDLYWRLAVVPIQVPPLRERKEDIPLLA
                     AHILARRRGAAKSFAGNEARYPTQITAKALQRLSGYRWPGNIRELENVLSRAAILCDG
                     EMIRSHDLAMLGLDASRVSGAPEEEDRIDMPPIDLERLGDGETLKDVTEKAVRAVERA
                     AIAAALRRDRNPAAAAKRLGISRASIYTKMKTYGLSPDGEATE"
     gene            10086..10613
                     /locus_tag="VMZ53_21185"
     CDS             10086..10613
                     /locus_tag="VMZ53_21185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS31044.1"
                     /translation="MTPTPTASAAELEKTRAYVKSVALKYVRNEQDADDVAQDAMLLA
                     HRYRESFRGESRYSTWLYRVTATAALMFLRRQRRLSRELPASGTTDDEGTPWLDRQPS
                     ATDTRAEVIARAELDDVRRAVAALGAKYPAVFWKRYGEGRTETEIAKELGISVAAVKT
                     RAFRGRQAALRCAAR"
     gene            10714..11037
                     /locus_tag="VMZ53_21190"
     CDS             10714..11037
                     /locus_tag="VMZ53_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31045.1"
                     /translation="MLHAIDRADIDGFYGEPAEFDVTLEVELLVIDAAAAAEFDDDRI
                     TTPIPRLREVALAAETISVDGLEDAWFGSFDENAELPADAFDDEPTTTWQKIGDWLKN
                     LGRAA"
     gene            11283..>11814
                     /locus_tag="VMZ53_21195"
     CDS             11283..>11814
                     /locus_tag="VMZ53_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31046.1"
                     /translation="MLLHLIPLVAVSMSANAATNAANAASDAADSAAMAERSAQVRLA
                     DMLGNVDSIDEVRAHGHTFTFLVEHADATGYDAPFAIVATTGDAGNVITVVEHARAAS
                     DMDLGGLSWLVDTMQQTIAVTSLDVDDDGSVTLTTNDGARYMAIPGRGSGGSGATADD
                     AGANIEVEARWAAEFDH"
BASE COUNT         2054 a         4068 c         3738 g         1954 t
ORIGIN      
        1 tcgcgcccgt gagccgcgcc acgccggccg agctccaggc caccacgacg aaggtccggg
       61 actacctgct cgccgtcgtc accgacgcgc aggccaagga ggccaagggc gagcccagca
      121 aggagcagag caaggagccc aggagcgagc tgacgcgcgc gcaggtccag gcgatcgtcg
      181 atccggacaa gctcgtcgac gcggcggtgc cgtatctcga gctcgagcgt gacgatctcg
      241 cccagctcct cgtcgagacc gacacgatga agcgcctcga gcggatcatt ccgtcgctcg
      301 agcgtcaagc cacggtgctc cgcctcaagg cagacatcgg cgccgagctc gaaggcgagt
      361 cgagtcgcaa ccatcgcgag cgcgtgctgc gcgatcgcat gcgccagatc caagaggagc
      421 tcggcgagca ggacgacaac gcggagatcg acgagcttcg caagaagatc gaagactcga
      481 agatgtccga cgaggttcgc gcggttgcca agaagcagct gtcgcgcatg agccagatgg
      541 cgtcgtcgtc gcccgagtac aacatcgcgc gcacgtacgt cgagaacctg ctcgagatcc
      601 cgtggaacgt gacgacggac gaccgcctcg acgtagcggc ggctcgcgca attctcgaag
      661 ccgagcactc cggtctcgag aaggtgaagc gccgcatcct cgagttcctc gcggtgcgca
      721 agctcgcccc gaacaagcac ggcccgatcc tgttgctcgt cggtcctccg ggcgtcggta
      781 agacgtcgct cggtcgctcg atcgcgagct cgctcggccg caagtacgtc cgcatctccc
      841 tcggcggcgt gcgtgacgag gccgaagttc gcggccaccg ccgcacgtat atcggcgccc
      901 tccccggccg catcgtggcg ggcctcaaga aggccggctc gatgaacccg gtgttcgtcc
      961 tcgacgagat cgacaagctc gctgccgata tgcgcggcga cccggcggcc gcgatgctcg
     1021 aggtcctcga cccggagcag aacaaggact tcgtcgatca ctacgtcgaa gtgccggtcg
     1081 acttgtcgaa ggtcatgttc atctgcactg cgaaccagct cgacacgatc agccagccgc
     1141 tcctcgatcg tatggagacg gtggagctgt ccggttacac gaccgtcgag aagctcgcga
     1201 tcgcgaagaa ccacctcctc ccgaagcagc tcggcgagca cggcatcagc aaagaccaga
     1261 tgcaggtcga cgacgatgcc ctcgaggaca tcatccacag ctacacgcgc gaggctggcg
     1321 ttcgtaacct cgagcgcgag atcgcggcag tctgccgtgg cgcggccgtg aaggtcgccg
     1381 aaggcgtcac cggcgtgcac gtcacgaagg agatgctgca ggagctgctc ggcccgccgc
     1441 gcttcgtgtc ggatacggcg gagcgcaaac ccgaggtcgg tgtcatcacc ggtctcgcgt
     1501 ggacgccggt cggcggcgac atcatgttca tcgaagcgcg ccgctatccc ggcaagggcg
     1561 acgttcgcct gacgggccag atgggcgacg tgatgaagga gtcggcgacg gccgccgtgt
     1621 cgtggactcg cgcgaatgca gcgcgcctcg gcatcgactc ggacaagatc gcgaacagcg
     1681 acctccacat ccacttgccg cagggcgcga tcaagaaaga cggtccgtcg gcaggtgtcg
     1741 cgctgacgtg cgcagtcgtg tcggtcttca cgggccggcc gattcgcaac gacgtcgcga
     1801 tcaccggcga gatcgacctc cgtggacacg cgctgccgat cggcggcgtg aaggagaagg
     1861 tcctggccgc gcatcgcgcc ggcatcaaga tcgtcttcat gcccgagcgc aatcagaagg
     1921 acacgctcga catcccggaa gagatccgga accagctcga cctccgcttc atgaagaaga
     1981 tcgacgacgc actcgaagtg gcgctcggac cgatgcctcc ggcgagcgag cccgagcccg
     2041 cgattccgcc gccgaccgct ccgagccgcg gctcgatggg cgaccgcctg ccgagctgat
     2101 ggctcgcgct gccctgctct tcctcgctgt cgctgcatgc agtggcagcg gtcgaggtca
     2161 caagccgccg agcgaggcgg agtgtcaggc gtatcgcgac aagttgttcg cgtacatgcc
     2221 cgaggcggac cgcacgaagg tcacggcgat gggccttggc aagcagacgc cgatcgagct
     2281 cgcgctctgc cagcagcgca tgaagtccga cgagatcgcg tgcgtcgtcg cgtcgaagac
     2341 attcgatgaa gcgctcctgt gcaagcccgc ggtcgacgat cggcccgcgg agatgaagcg
     2401 gtcaccggcc gagtgccagg ccttcatgga gcacgtcacg aagctcgcga acgatgccgc
     2461 gcaaggcgat gcgagcggcc cgccgttcac gccattcatg gcgaagctca cggcgcgtga
     2521 gtgtgggcgg tggctgtcga aggagcgcta cgagtgcgtg atgcaagcgc cgacaacgct
     2581 cggcatcgtt ggctgcccgc cctagatcgg tggtcgcgac gaatactgca gctcgatcca
     2641 atcgagcgcg gtcggtatca cgccatcacc ggtgaattcg accgcgtact gcgcgtacgg
     2701 cttcaacgca acgcttggca cggcacccga ttgcgggacg tcggtccacg cctctcccgc
     2761 gcaggacgca tccgcgcaca tgcggaactg caccttcgcc gcgctgccct gtcgcgtctg
     2821 ccacgagagc ggcccgagtg cgacgacgtt gccgagatcc ttcggcgctg actcgtagcg
     2881 atacgtcgtc gggaatggct tcaggccgcc gtcgtacagc atcgtcacgc tgaacagatc
     2941 gagcgtcccc gtgatggcat cgacggtgct gtcgctcgcg atcagattcc acgaggctcc
     3001 cgcgtcagcg gcaggaaccg tcacgtgctc gacatgctgg cccgaacccg cgagtgcacc
     3061 ggtcgcgacg agcgtgatgt tgctgccggc gggcggatcg agcgtgatgc cgacctgcgc
     3121 ctgcgccgtg tgattgaatt cgaaatccgc gtcgatgctc ttcgccatcg cgccgttcgg
     3181 caggagcaac gtcagcggcc gcatgatgct tccgccgtcg gtgatcgtcg tcgacgctgc
     3241 atcgctatcg gccgcccagc ggaacgcgtt gctcggcacc ggtcgcagat gatcgaccgg
     3301 gaagctctgg ccgaccaagt caggaccgct cgcgacgacg atcgacgctg tgagcagcgc
     3361 cgagctattt gccttcatcg cgtcgaacac gaggtagtgc caaccagcat cgagatggat
     3421 cggcgcgacg ttcgttgtcg catcggtccc gccgaatgca ttcgcaacct gctggccgtc
     3481 gatccatacg cgatgaccgt gatgcgacga gaccgtaaag ctgtagtcgc cttccttgtc
     3541 gatcaagaac tgccccaccg cgcgcatcga caagctcaag atgccgatgt tgatcgcata
     3601 tggatcgtcg ggcagcgtca gctggatcgg cgtcgcgagc agcgtcgagc cgaagtagcg
     3661 cagcaagtac gtatcgtcga agccgtcgaa gattgcgcca tcgatgccgt cgacgcgcac
     3721 gcgcatctgg tctggtgcga tctcgcgaaa cccgccctgg atcgccggcg agtccatgcg
     3781 gacgtgaaag tccatgaaca tcgccgcgtc tgcaaacgcc gcgcgcaagc gataccagcc
     3841 cggcgtcgcg acgttgtacg tggcgctcgt tgcggcattc gcgctgttca cgacgcgcgt
     3901 gaagtcgccg ccaggaggcg cgagctcgag gaagccgttg tcgttcgcgt cgagctcgaa
     3961 gcgccacgcg ccggccgcat cgagctggat ctcgcactcg acgaggaccg tcacgtcgtc
     4021 ggcttgtgtg agccccacac cgcgcggcac gaggatgtcg taattgagcg cgagcgcggt
     4081 cgcgaatgat gtccccgcgc gcgcgacgtc gacgttcgcc catgtggctg cggcggcatc
     4141 ggcgccgatc ttgttgccca ccacgcccga taccttcgcg cgcccggtcg cgtacggcac
     4201 cggcccgacg aagcccgcct tctcgaccgc actgtcgatc agcacggcgc cggtctgcga
     4261 aaaatcagcg gcagagtcgt cgcgccacgt gcgcagctcc gcgggctcac cacacgtgtt
     4321 cgagatcgtg tcgcactcct gcccgcgcgg gcactcgccc gtgtccgagc acggaatgcc
     4381 tagcggcacc tgcgggtcgt aacaaccgac cgcggcaaac agcgccgcgc cgagcgtcca
     4441 tcgcacgcga cgatgatacg ccagctagct cgacggcacg agctgataga tcgagcctgt
     4501 aagctcgacg atatacagct cgccatcgtg atctacgcca aacgagctca cctgcgtgat
     4561 cacgccctgc ttgtcgatcg cgggcttcca gtcccagtgc tctcgcaccg caccgctcac
     4621 ccacacgaag ctgcgcagta ggcccgtgca gtagtcggcg tagaagtacc gacccgcgag
     4681 cgccggcaac gccttgccgc gatacacgta gccgccggtg atcgagcagc cctgctcgtg
     4741 cggatactcg acgagcggcg gcgtgagccc ggtccgatcg catgtctgct tgctcgtgcc
     4801 gctgtccgga tcgaagcagt gcgtgccttc gacgatgttc cagccgaagt tcttgcgcga
     4861 cgtgtcggtg cccgcgacgg cgtggacgta ctcgaacaga ttctggccga catcgccgat
     4921 gaacaagtcg ccggtcttgc tgtcgaacga gaaccgccac ggattgcgca gcccgatgtg
     4981 cacgatctcc ggcttcgcat caccatcgac atcgaagcgc agcatcttcg cgagcagcgc
     5041 cttttcgttc tgaccattgc cgtgcggatc gttcgcggcg ccgccatcac ccatgccggc
     5101 gtagagcttg ccgtcggggc cgaacgcgag atggccgccg ttgtggttcg agtacggctg
     5161 ctcgatgcga atgatctcgc gccgcgatgc ggtgtcgacg acatctggat cgctcgccga
     5221 gactttgtac tcgacgatgt gcgtcgacat gtccttcgcg gtgtagttga cgtagagctt
     5281 cttggtctcg gcaaagcgtg gatgaaacgc gagaccgagc aggccctctt cgttgccatg
     5341 cgagagcccg tcgatcgtga agaacgcctt gtcggagacc ttgccgccct cgaggatctt
     5401 gatcgcgcca ggctgctcga cgatgaacaa tcgtcgacga ggatcgttcg gcgcgctcgt
     5461 cagcagcacc gggcgcttca gcccgtgcgc aatctccttc aacgcgacct tcgccgcgat
     5521 ctcggccggc ggcgcgacga gctcgtcggt gccaggtggc ggcgtcgtcg ccggcgcgac
     5581 gctggcggag ccgcttccac ctagctgcgt cggtttgggc tcaggtccgc ggctcggcgc
     5641 gcgtccatct ccgcgacacg ccgtcgcgaa caacaacgtt acaagggcaa gtctcacagt
     5701 tggcaagtcg tgccatgact ccaatgtgtt agcgagctga ctggctaaaa ctgaagccgc
     5761 tttgatgcaa ggacacagca atccagccgg ctagtgcagg agccaccccc ttgcgccgcc
     5821 gacgcaaacc atgggatcga cgcggctcct cgccgtgggg tctgtaggcc caagtggcac
     5881 gcagaatgct catcggcagg cccaggagta acgccatgca ggtcaacgcg catatcgctc
     5941 gcaagaacac gccggtcccg atgatcgcgg gccacgccaa tgcgaagaac agcaaggacc
     6001 tgcagcgcta cttcgagtac tccgcgtcgg gagtcgacgt ggtcgaagat ggcctcaagg
     6061 agatcacctt cggcgagttc ctcgtcgagc agcaggtcct cgaccgcttc cagctcttcc
     6121 gcgcgctcca gatgcaggac cgccttcccg gcgttcgcct cggcgagtgc gccgcagcgc
     6181 ttggctacgc gccgatcaac gcgatcgagc gcctctacga gcgcttcgcg cagctctcga
     6241 ccgtcgacgt tccctagtca gataaagcag tcgtactgct cttggcgaaa cacgcgcccg
     6301 tctcgaagct cgatcgcgct gcagatgtga gccttcatct cgcgcccatc ggctagcacg
     6361 ccggtccaca ggatctgcgc cacgcagtga tcgccctcga cggtcatcgc gcgcacctcg
     6421 tagcgctcgc tcgcgagcag cgccttgccg cgttcgcctg cctcgcgcag cgccgccttg
     6481 tcgtacgtct tgccggcggg gttgagcttg ttcggatgct cggtgacctg cacgtcgtca
     6541 tgcagaaatt gcgcgacgag atcgaggttg tgcgactcga tcgcgtgcag gtaggcgagg
     6601 acggtttcct tcgtgctcat ggcgcttcga tgccgctaat cgacgccgcg tatcaccatc
     6661 gtgccgctcg cgtcggggct catgtccatc gcgcgaatga cggcctcggt gtactgcatc
     6721 gtgttcgcct taccgccgag gtcgccggtg cgcgtctcgg ccttcgcaag cgcggctttg
     6781 attccgcgac gcaggcggtt ggcggcgtcc cgctgctcga ggtgatcgag catgagcgca
     6841 gcggacagca tgaggcccgt cgggttcgcg atgcccttgc ccgcgatatc aggcgcggtg
     6901 ccgtgcacgg cttcgaacat cgcgatgccg ccgtcgccga tgttggcagc gggcgcaacg
     6961 ccgaggccac cgacgagacc tgccgtgagg tccgacaaga tgtcgccgaa caggttcatc
     7021 gtgacgatca cgtcgaagcg ctcgggctgg agcacgagct tcatcgcggt ggcatcgacg
     7081 atcatgtcgt cgaactggat ctcgggatag cgcttcgcga cttccttgcc ggtgtcgagg
     7141 aagaggccgt tggacatctt gaggatgttg gccttgtgga cgagcgtgac cttcttgcgg
     7201 cccatgcggc gcgcgtactc gaacgcgtag acgatcacgc gctcgctgcc gaacttcgtg
     7261 atgatcgcga tcgattcggc ggcggtgcga cgcggatcga cgtagtgctc gacgcccgcg
     7321 tacaggtcct cggtgttctc gcgaacgatg acgaggttca cacccgtgta gcgcgacggc
     7381 acgcccgcga tcgtctcgac cggccgcacg ttcgcgtaga gatcgaagtg ctggcgcagc
     7441 gtgacgttca ccgagcgata gccgccgccg ctgggcgtcg cgagcgggct cttgagcgcg
     7501 atgccgttct tcttgatcga ttcgatcgtc gcaggaggca gcggatcttt atgccgctcc
     7561 acggcgatcg cgcccgcgtc tgccctctcc cacgcaatct tgccgccggc cgcttcgatc
     7621 gcacgcacgg tcgcagcagc gatttcgggg ccgatgccat caccttcgat cagtgtcacg
     7681 gtcttcattc aatatcctcg tagcgttcgg ggaacaggat cgacttcgat cgtctcgccg
     7741 ttgacgcgaa cagcataggt gacgagacaa gcatcagatg atgaatcgtg gcggccggtc
     7801 gcagtcgaaa accgccagcc atgattcgcg cagatgacgt gaccgcgcga cacgataccg
     7861 tcggcgaggt cgccgccttg gtggacgcat tgccgctgca tcgcgaaata gcgctcgcca
     7921 tcgcggccga cgatcatgga gacgccttcg acctcgaggc cgatcacgtc gcctgcctgg
     7981 agctggccca ccttcgcgac ggcgtgccac gccatcgcta cgccttcgtc cacaccgcga
     8041 gctcgcagcc gcccggatcg acgaagtgaa agcgccgccc gccgggaaac tcgtgtcgct
     8101 cggtgatcgt tgcgccggca gcggtgaccg cgcgctcgct cgcctctaga tcatcggagt
     8161 agacaatgag cagcgggcca ccagaggtcg gcggcgcctt gaccagtcgc agcccgcccg
     8221 tcatgcccgc gccctggacg tccgcgtaat cgggaccgta gtccttgtag ctccaaccga
     8281 acgccgcgcc gtagaacgcc ttcgacccgg ccatatcgag caccgggatt tcgaagaagt
     8341 cgaccgtgtg gtgagtcggc attgtcgcga cgaggatacc caggctcgct cgcgcgcgca
     8401 caagctcccg acgctaatcg aatgcgccgc tgcgcctcac tccgtcgcct cgccgtcggg
     8461 cgagagcccg tacgtcttca tcttggtata gatgctcgcg cgggatatcc cgagccgctt
     8521 cgccgccgct gctggattcc gatcgcgccg cagcgccgcc gcaatcgccg ctcgctccac
     8581 cgcgcgcacg gctttctccg tgacgtcctt gagcgtctcg ccgtcgccga gcctctcgag
     8641 atcgatcggc ggcatgtcga tgcggtcttc ctcttcgggc gcaccgctca cgcgactcgc
     8701 atcgaggccg agcatcgcga ggtcgtggct gcgaatcatc tcgccgtcgc acagaatggc
     8761 cgcacgcgag agcacgttct cgagctcgcg aatgttgccc ggccaccgat agcccgacag
     8821 ccgctgcagc gccttggccg tgatctgcgt cggataccgc gcctcgttgc cggcaaagct
     8881 cttcgcggcg ccgcgtcgtc tcgcgaggat gtgcgcggcg agcagcggaa tgtcttcttt
     8941 gcgctcgcgc agcggcggca cctgaatcgg cacgacggcg agtcgccagt acaagtcctc
     9001 gcgaaactgc ccgcggccga cgaggttctt cagatccttg tttgtcgcag cgacgacgcg
     9061 cacgtcgatc tgcacgggcc tgtcaccacc gaccggcttg atctcgcgct cctgcaacac
     9121 gcgcagcagc ttgttctgca cgctcgcgtc gatgtcgccg atctcgtcga gaaagatcgt
     9181 gccgccgttc gcctcgcgga acagcccgcg ccgcgcacgc atcgcgccgg tgaacgcgcc
     9241 cttctcgtgc ccgaacagct ccgcttcgat gaggctcggc gcgagcgcgc tgcagtcgaa
     9301 cgcgacgaac ggcccatcct tgcgtggccc gacgcgatga atcgtgcgcg cgagcagctc
     9361 cttgccagtc ccgctctcgc cgacgatcag gaccgtcgca tccgacgccg cgacgcgctc
     9421 gatcacttgc aggagctgcc gcatcagcgg cgactcgccg atcacctcgc ccaatccgcg
     9481 tgccttctcg acctcgccgc gaagccgccg cacctcgcgc gcgagtgcac cgtgctgaat
     9541 ggcgcgatcc accacactgc gaatctccgc ggtcgccgcc tctttatgca gcacgtcgag
     9601 cgcgcccgac cgcatcacgc gcaccgtgaa ctcgacggtc ggcttcgcgc tccagatcac
     9661 gaccacggta tcgggcgcga cagtcctcgc tcgatgcagc agcgcgagcg cgccgtcctc
     9721 gtcatcgccg agcgcgggat cgagcaacac gagatcgaac gggctgccga cgagcctctc
     9781 gagcatggcc ggcgctccgc ccacctcctc cacatccacg cccgccaacg cctctcgcgc
     9841 gtgcgccaca ccggcgggat cggcgtccgc caccaacacg tgccgcactc catctacgcg
     9901 aagcgccgtt tcggccacga ctacagtctg tccagccgtc tagacactgt ccagccccct
     9961 ggacgaatgt ctggacgatc agacacctaa gatggcgtaa ccacttgtcc tcgccgttgg
    10021 acagacctgg cgctgctgtt gcgtattggt cttcagcgag gagggtgacg atgcaagcga
    10081 cctcgatgac tccgacccca accgccagcg ctgccgagct cgaaaagacc cgcgcctacg
    10141 tcaaatcggt cgcgctcaag tacgtccgca acgagcagga cgccgacgac gtcgcgcagg
    10201 acgcgatgct cctcgcgcac cgctaccgcg agtcattccg cggtgaatcg cgctactcga
    10261 cctggctcta ccgggtgacg gccaccgccg cgctgatgtt cctgcgccgc cagcgccgcc
    10321 tctctcgcga gctcccggcc agcggcacga ccgacgacga aggcacgccc tggctcgacc
    10381 gccagccgtc cgctacagac acgcgcgccg aagtgatcgc ccgcgccgag ctcgatgacg
    10441 tccgccgcgc agtagccgcc ctcggcgcga aatacccggc cgtgttctgg aagcgctacg
    10501 gcgaaggccg caccgaaacc gagatcgcca aagagctcgg aatctccgtc gctgccgtca
    10561 aaacgcgcgc attccgcggc cgccaagccg cactccgctg cgccgcgcgc taacgccccg
    10621 cgcccgtcga gcacttgcct cgggtagcgc tacccgatcg catccccgct gctttcttgc
    10681 acgttgccgc aggcatgaga cgttcaactg gtagtgctgc acgcaatcga ccgagcggat
    10741 atcgacggct tctacggcga gcccgccgaa ttcgacgtca cgctcgaggt cgagctcctc
    10801 gtcatcgacg ccgccgcggc cgcggaattc gacgacgatc gcatcacgac gcccataccg
    10861 cgcctacgcg aagtcgcgct cgcagccgaa acgatttccg tcgacggcct cgaggacgcg
    10921 tggtttggca gcttcgacga gaatgccgag ctcccagccg acgcgttcga cgacgagccg
    10981 acgacgacct ggcagaagat cggcgactgg ctgaagaacc tcggccgcgc agcctgaaat
    11041 cagccgcctg ctcgtcgcgc agccggaaat tcagtccgac ctaattaatc gcgcggctcg
    11101 gaaattcagt ccgacctaat tattcgcgcg gctcggaact tcagtccgac ctaattgttc
    11161 gcgcggcgca gcggacacgg ccctagcgga gttagccgcg tgtccgtgca gccacgccgg
    11221 ccgctaagtg ggcgcggcgg ttggcactct tggctggcgc ggcggtcgca gtacccctcg
    11281 gcatgctgct tcacctgatc ccgctcgtcg ctgtctcgat gtcggcgaac gccgcgacca
    11341 acgccgcgaa cgccgcgagc gacgccgctg acagcgcagc gatggccgag cgcagcgcgc
    11401 aagtgcggct cgccgacatg ctcggcaacg tcgactcgat cgacgaagtc cgcgcgcacg
    11461 gtcacacgtt cacgttcctc gtcgagcacg ccgacgcgac gggctacgac gcgccgtttg
    11521 cgatcgtcgc gacgacggga gatgccggca acgtgatcac cgtcgtcgag catgcgcgcg
    11581 ccgcgagcga catggatctc ggcggcctgt cgtggctcgt cgatacgatg cagcagacga
    11641 tcgcggtcac gagtctcgac gtcgacgacg atggcagcgt cacgctgacg acgaacgacg
    11701 gggcacgcta catggcgatt cccggccgcg gctcgggcgg cagtggcgcg accgccgacg
    11761 acgcgggcgc aaacatcgag gtcgaggcgc gctgggccgc cgagttcgac cacg
//
LOCUS       DATLBZ010000417         5567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4214768, whole genome shotgun sequence.
ACCESSION   DATLBZ010000417 DATLBZ010000000
VERSION     DATLBZ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4214768"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VMZ53_21200"
     CDS             complement(<1..436)
                     /locus_tag="VMZ53_21200"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831103.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HUS31047.1"
                     /translation="MDVRKIREILGEKASLLEHQSKTIPASSLTLPSPRYVDEIYASS
                     DRSPRVLGSLQRMFDHGRLGNTGYLSILPVDQGIEHSAGASFAPNPQMFDPEHIVKLA
                     IEGGCSAVASTYGVLGIVARKYAHKIPFIVKINHNELMSYPNT"
     gene            515..1453
                     /locus_tag="VMZ53_21205"
     CDS             515..1453
                     /locus_tag="VMZ53_21205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31048.1"
                     /translation="MRLSLAFTVASLSVLPLAACGGDDSNTTPDAPEDESGFATPTVT
                     LKANMEVSDDNWTEIGPANLACLNTATSDTPTATSVTLHTKVTDFQSGNSVPSATVVA
                     FKGQDQAMMFDTKTSDASTATLDIVIPAGVTRYGFKMTSSSALPTLLLNQIVNPAKVT
                     NMETTDPNKIQSVSNATAALLPALIGQTRTPGTGVVAGALRDCDGKEVSGFIAAVSST
                     PGTNTPIAGAQAYYFSDSVGLPQHHREAPYASKDGLFMIIQLPATLGTGYVQMWGFPT
                     DADLAMGKAGLKLIAELQVPLIADTVITGSYEPKRN"
     gene            complement(1463..1978)
                     /locus_tag="VMZ53_21210"
     CDS             complement(1463..1978)
                     /locus_tag="VMZ53_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31049.1"
                     /translation="MAASFSARALLAVALLSACGGGDGDDAPPAGTCIPDGYWTWPAD
                     CMTAVCDGIVCDATCEVGDTCERLDCTKSPQCRVECQDQTHCSDIDCRGGTFCFATCL
                     GAGTSGGDCTMHGEGAGAATIECLDGATCLIECGATPSSACRISNCTGGSGITDCGGG
                     VSVCNRPCPQR"
     gene            2035..2796
                     /locus_tag="VMZ53_21215"
     CDS             2035..2796
                     /locus_tag="VMZ53_21215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HUS31050.1"
                     /translation="MTKLPAEAPAEPERKAGRDALIAYVVVAIAVTGLVRINITLPAI
                     GHLGSALVSVVFLYAPIIYLGRFRDEDIYDYGFRWQPLGKSLGTALVAIAIIFPLFAV
                     GYFAFYEVACHSKQLVHLVPRNMCVHYGGLHAIHAPAMSGKLAEFILVQLLVVALPEE
                     LFFRGFLLGLLEKRFPPKRRFLGGGIGLALVLSSLAFALIHLPKDGDPRVLATFFPGL
                     LFGWMRSRTKSIVASTVAHGFSNILIRILDLAAMR"
     gene            2946..3149
                     /locus_tag="VMZ53_21220"
     CDS             2946..3149
                     /locus_tag="VMZ53_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31051.1"
                     /translation="MTTIDNQILETVTGGTQTVSGARKLDTATEQALTKLSSDVKDLA
                     KAPQQSQTTQLMTVMMMAKMMRG"
     gene            3186..3833
                     /locus_tag="VMZ53_21225"
     CDS             3186..3833
                     /locus_tag="VMZ53_21225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HUS31052.1"
                     /translation="MRAAFFDMDNTLLTVDTGMSWTRFLYRRGELPPSMVAKAIYWST
                     LYKLAVLDMESVFTKLCMDLAGDSEAEMIAKCDVWYREHVAPFVAPAARVAVEHHRQR
                     GDMIVLATGSTVYAATPVARDMSIEHVLSSRLEVEAGAFTGRPSALCFGHHKVTLAEK
                     WAADHGIDLAASTFYSDSFNDLPLLERVGTAVAVNPDARLRRHAQRRGWALQRWS"
     gene            3853..4338
                     /locus_tag="VMZ53_21230"
     CDS             3853..4338
                     /locus_tag="VMZ53_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31053.1"
                     /translation="MRLVLVLLFALVSTRAAHADVYPSKAGKVSIDVPKKWTVNATDE
                     LVRASSPDNAVAFVFWVIDSPDVKPAVKKLEGELYSSIQGLRWVDKTKKLKINGLPAT
                     WVEGVGVNAAESQLDVLVVVAGPTKTKKGVLMLAVVEHDKLKANEKTIQSIFKTLKAT
                     K"
     gene            4394..5260
                     /gene="nadC"
                     /locus_tag="VMZ53_21235"
     CDS             4394..5260
                     /gene="nadC"
                     /locus_tag="VMZ53_21235"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HUS31054.1"
                     /translation="MGAFALRGLAAIPAVRRLIELALDEDLGRGDITSSVTLGPDGRE
                     VVADMNAREPIVAFGVDVAAAVFALVDPRIELEIHSPDGSRVDRGGLLLTARGPAHNV
                     LAAERTALNFVQRLSGVATQAKRYADAVAGTKARVVDTRKTTPGYRVLEKAAVLAGGC
                     GNHRFDLGSGILIKDNHIAACGSVRAAVEAAKVRAPHPLRIEVEVTNLGELDEALAAG
                     AEIVLLDNMTPSQVEVAAARAHSRGVLVEVSGGITLATIADYARSGADLISVGALTHS
                     APAVDIGLDVRT"
     gene            5343..>5567
                     /locus_tag="VMZ53_21240"
     CDS             5343..>5567
                     /locus_tag="VMZ53_21240"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31055.1"
                     /translation="MTFEALTLKARWLGCQRIDLESCASTNDEAARLARAGASRGTVV
                     IAERQTAGRGRDGRVWESPPGGLYMSIVLRP"
BASE COUNT          938 a         1873 c         1809 g          947 t
ORIGIN      
        1 acgtgttcgg gtagctcatc agctcgttgt ggttgatctt cacgatgaac ggaatcttgt
       61 gcgcgtactt gcgcgcgacg atgccgagca cgccgtacgt cgacgcgacc gcgctgcagc
      121 cgccttcgat cgcgagcttc acgatgtgct ctggatcaaa catctgcggg ttcggcgcga
      181 acgacgcacc ggcggagtgc tcgatgccct gatcgaccgg caggatcgac aggtagcccg
      241 tgttgccgag gcggccgtgg tcgaacatgc gctgcagcga cccgagcacg cgcggcgaac
      301 ggtcgctcga cgcgtaaatc tcgtcgacat agcggggcga tggcagcgtg agcgagctgg
      361 ccgggatggt cttgctctgg tgctcgagca ggctggcttt ctcgccgagg atttcgcgga
      421 tcttgcggac gtccatgtgc gcttgtttac ccttcgatcg gaaatggttg caatgacggc
      481 cgccccctcg ctaaaagcgt tctcgggagt tgccatgcgt ctatctctag ctttcaccgt
      541 tgcctcactg tccgtcctcc ctctagcggc ctgcggaggt gatgacagca acaccacgcc
      601 cgacgcgccc gaggatgaat ccggcttcgc gacaccgacg gtgacgctga aagctaacat
      661 ggaggtctcc gacgacaact ggaccgaaat cggcccggcg aacctcgcat gcctcaacac
      721 ggcgacgagc gatacgccga cggcgacctc ggtgacgctc cacacgaagg tcaccgactt
      781 ccagtcgggt aactccgtcc cgagcgccac ggtcgtcgcc ttcaagggcc aggaccaggc
      841 gatgatgttc gatacgaaga cgtcggatgc atcgacggcg acgctcgata tcgtgattcc
      901 ggcgggcgtg acccgctacg gcttcaagat gacgtcgagc tcggcgctgc cgacgctgct
      961 cctgaaccag atcgtgaacc cggcgaaggt cacgaacatg gagacgaccg acccgaacaa
     1021 gatccagtcg gtctcgaacg cgacggccgc gctcctgccc gcgctcatcg gccaaacccg
     1081 cacgccgggc accggtgtgg tcgccggcgc gctccgcgac tgcgacggca aggaagtctc
     1141 tggcttcatc gccgcggtgt cgtcgacgcc gggcacgaac acgccgatcg ccggcgcgca
     1201 ggcctactac ttcagcgact cggtcggcct gccgcagcac caccgtgaag cgccgtacgc
     1261 ctcgaaggac ggcctcttca tgatcatcca gctgccggcg acgctcggca ccggctacgt
     1321 ccagatgtgg ggcttcccga cggacgccga cctcgccatg ggcaaggcgg gcctcaagct
     1381 catcgccgag ctgcaggtgc cgctgatcgc cgacacggtg atcaccggtt cgtacgagcc
     1441 gaagcgcaac taatcacgac ggtcagcgct gcgggcacgg ccgattgcag acggacactc
     1501 cgcctccgca atcggtgatg ccgctgccgc cggtgcagtt cgagatccgg cacgcgctgc
     1561 tcggcgtcgc gccacactcg atgaggcacg tcgcgccgtc gaggcactcg atcgtcgctg
     1621 cgccggcacc ttcgccgtgc atcgtgcagt cgccgcccga tgttccggca ccaaggcacg
     1681 tcgcgaaaca gaacgtgccg ccgcggcaat cgatgtcgga gcagtgcgtc tggtcttggc
     1741 actcgacgcg gcactgcggt gacttcgtgc agtcgagccg ctcgcacgtg tcgccgacct
     1801 cgcacgtcgc gtcgcacacg atgccatcgc aaactgcggt catgcagtcg gccggccacg
     1861 tccagtaacc gtcggggatg caggttccag cgggcggcgc gtcatcgccg tcgccgccac
     1921 cacatgcgga tagcagcgct acggcgagta gcgcacgcgc agagaagctc gcggccatga
     1981 cgtagcgagt ataacctcga cgagcacgtg cccgagccgc tgcccccagc gactgtgaca
     2041 aagcttccag ccgaggctcc tgcggagccc gagcgcaaag caggccgcga cgcgctgatc
     2101 gcatacgtcg tcgtcgcgat cgcggtcacc gggctcgtgc gcatcaacat cacgctgcct
     2161 gcgatcggtc acctcggcag cgcgctcgtc tcggtcgtct ttctctacgc gccgatcatc
     2221 tatctcggcc gattccgcga cgaggacatc tacgactacg gcttccgctg gcagccgctc
     2281 ggcaagagcc tcggcaccgc gctcgtcgcg atcgcgatca tcttcccgct cttcgcggtc
     2341 ggctacttcg cgttctacga agtcgcgtgt cacagcaagc agctcgttca cctcgtgccg
     2401 cgcaacatgt gcgtgcacta cggcggcctc cacgcaattc atgccccggc gatgagcggg
     2461 aagctcgccg agttcatcct cgtgcagctg ctcgtcgtcg cgctccccga ggagctgttc
     2521 ttccgcggct tcctgctcgg cctgctcgaa aagcggttcc ctccgaagcg ccgcttcctc
     2581 ggcggcggca tcggcctggc gctcgtgctg tcgtcgctcg cgttcgcgct cattcacctg
     2641 cccaaagacg gcgacccgcg cgtcctcgcg acgttcttcc ccggcctctt gttcggctgg
     2701 atgcggtcgc ggacgaagtc gatcgtcgcc tcgacggtcg cccacgggtt ctcgaacatc
     2761 ctgatccgga tcctcgatct cgcggccatg cgttaagtcg agtagccgtc accggcgagt
     2821 ccggtcgtca tgggcaaaca ggtggggact cgagctgccc tggcgtcccg cggacttgcg
     2881 caaggcggtg ttttctcgag gacgcgcgct gtcacgtggc acatcgaatg ctcctttccg
     2941 gcggcatgac gaccatcgac aaccagatcc tcgaaactgt caccggcggg acgcagactg
     3001 tctccggcgc tcgcaagctc gacaccgcga cggagcaggc gctgaccaag ctgtcctctg
     3061 acgtcaaaga cctcgccaag gcgccgcagc agtcgcagac gacgcagctg atgacggtga
     3121 tgatgatggc gaagatgatg cgcggctagc ggcaacgacc tgcgcttgct acggtcgcgc
     3181 ggcgcatgcg tgccgcgttc ttcgacatgg acaacacgct cctgaccgtc gacacgggca
     3241 tgagctggac gcgcttcctc tatcgccgcg gcgaattgcc gccgtcgatg gtcgccaagg
     3301 cgatctattg gagcacgctc tacaagcttg ccgtgctcga catggagagc gtgttcacga
     3361 agctctgcat ggacctcgca ggtgactccg aggccgagat gatcgcgaag tgcgacgtct
     3421 ggtatcgcga gcacgtcgcg ccgttcgtcg cgcccgcagc gcgcgtcgcc gtcgagcatc
     3481 atcgccagcg cggcgacatg atcgtgctcg cgacgggctc gaccgtgtac gccgcgacgc
     3541 cggtcgctcg cgatatgagc atcgagcacg tgctgtcgag ccggctcgaa gtggaggccg
     3601 gcgcgttcac cggccggccg agcgcgctgt gcttcggtca ccacaaagtg acgctcgccg
     3661 aaaagtgggc cgccgatcac ggcatcgacc tcgccgcgag cacgttctac agcgacagct
     3721 tcaacgactt gccgctgctc gagcgcgtcg gaaccgccgt cgcggtgaat cccgacgcgc
     3781 gactgagacg ccacgcacag cggcgcggct gggcgctgca acgctggagc tagagctgct
     3841 aggttagagc cgatgcggct cgtgctcgtt ctgctattcg cgctcgtctc gacgcgcgcc
     3901 gcgcacgccg acgtctatcc gagcaaagca ggcaaggtct cgatcgacgt cccgaagaag
     3961 tggacggtca acgcgaccga cgagctggtc cgcgcctcgt cgcccgacaa cgcggtcgcg
     4021 ttcgtgttct gggtcatcga ttcgcccgac gtgaagcccg cggtgaagaa gctcgagggc
     4081 gagctctaca gctcgattca aggcctgcgc tgggtcgaca agacgaagaa gctgaagatc
     4141 aacggcctgc cggcgacgtg ggtcgaaggc gtcggcgtga acgccgcgga gagccagctc
     4201 gacgtgctcg tcgtggtcgc gggcccgacg aagacgaaga agggcgtgct gatgctcgcg
     4261 gtcgtcgagc acgacaagct caaggcaaac gagaagacga tccagtcgat tttcaagacg
     4321 ctgaaagcga cgaagtagcg cgtcgaattt tggtgattgc agcgtcagat tgatctagta
     4381 gggtccccgc tgtatgggtg cttttgctct tcggggcctc gctgccattc ccgctgtccg
     4441 ccggctcatc gagctggcgc tcgacgagga tctgggccgc ggagacatca cctcgtcggt
     4501 gacgctcggt cccgatggcc gcgaagtcgt cgccgacatg aacgcgcgcg agccgatcgt
     4561 cgcgttcggc gtcgacgtcg cggccgcggt gttcgcactc gtcgatcctc ggatcgagct
     4621 cgagattcac tcgcccgatg gctcgcgcgt cgatcgcggc gggcttctgc tcaccgcgcg
     4681 cggcccggct cacaacgtgc tcgccgcgga gcggaccgcg ctgaacttcg tgcagcgcct
     4741 gtcgggcgtc gcgacgcagg ccaagcgtta cgcagatgcg gttgcgggga caaaggcgcg
     4801 cgtcgtcgat actcgcaaga cgacgccggg atatcgcgtg ctcgagaagg ccgccgtgct
     4861 cgcgggtggc tgcggcaacc atcggttcga tctcggcagc ggcatcctca tcaaggacaa
     4921 tcacatcgcg gcctgcggga gcgttcgcgc cgccgtcgag gcagcgaagg tccgcgctcc
     4981 gcatccactg cggatcgagg tcgaggtcac gaacctcggt gagctcgacg aggcgctcgc
     5041 ggccggagca gagatcgtgc tgctcgacaa catgacgccc tcgcaggtcg aggtcgcggc
     5101 ggcgcgcgcg cacagccgcg gcgtgctcgt cgaggtttcg ggcggcatca cgctcgcgac
     5161 gatcgccgac tacgcgcggt ccggcgcgga cctcatctcc gtcggcgcgc tcacgcactc
     5221 ggcacctgcc gtcgacatcg ggctggacgt ccggacatga cgatcccgga acactggatc
     5281 tcgacaatga tcctcgacga gggtgtcaga ccgatcgatg atgctcgtca acgggtgaag
     5341 ttgtgacctt cgaggcgctg acgctcaaag ctcgctggct cggctgccag cgcatcgatc
     5401 tcgagtcctg tgcctcgacg aacgacgagg cggcgcgtct tgcccgtgcg ggtgcgagcc
     5461 gcggcaccgt cgtgatcgcc gagcggcaaa ccgcaggacg cggtcgcgat ggccgggtgt
     5521 gggaatcgcc accgggcggc ctgtacatgt cgatcgtgct gcggccg
//
LOCUS       DATLBZ010000418         3987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6965365, whole genome shotgun sequence.
ACCESSION   DATLBZ010000418 DATLBZ010000000
VERSION     DATLBZ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6965365"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /gene="gmd"
                     /locus_tag="VMZ53_21245"
     CDS             complement(<1..903)
                     /gene="gmd"
                     /locus_tag="VMZ53_21245"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HUS31056.1"
                     /translation="MKKALITGVTGQDGSYLAELLLAKGYEVYGVVRRSSSFNTERLE
                     GIYQDPHVQDYRLRLVYGDLEDGASLANLVKKIQPDEIYNLAAQSHVRVSFDVPEYTC
                     ATVAMGTLRLLEAVREMDVKAKFYQASSSEMFGSAKPPQNEDTKFEPRSPYACAKVFA
                     HQLCQNYRDAYGMFICCGILFNHESPRRGIPFVTRKITRAAARIKHGLDKKLFLGNLD
                     AKRDWGFAGDYVEAMWLMLQQDKPDDYVIATGESYSVRDCLDVAFGTLGLDWQKYVEL
                     DPRYLRPTEVDHLHGDASKAREKLG"
     gene            complement(900..1841)
                     /locus_tag="VMZ53_21250"
     CDS             complement(900..1841)
                     /locus_tag="VMZ53_21250"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HUS31057.1"
                     /translation="MSSFSWSQKRVLVTGGAGFFGSFVVDELKRRGVTDVFVPRSSEY
                     NLVDRAACKRALADSRPDVVFHLAARVGGIGANRDNPGGMIFDNAMMGLQLIEECRLA
                     KIPKLVISGTICAYPKFAPIPFREDDIWNGYPEETNAPYGVAKKLLLVQSQAYREQYG
                     MNSIVLFPVNMYGPRDNFDLRTSHVIPAMIRKFLSARDEGKTSVTLWGDGSPTREFIY
                     APDAAEAFVTAAERYDSSDPVNIGSGEEISIKDLATLVAKATGFTGTFVWDTAQPNGQ
                     PRRRLDVSRAKERFGFVAKTSFAAGLEQTVAWYLENR"
     gene            1906..2847
                     /locus_tag="VMZ53_21255"
     CDS             1906..2847
                     /locus_tag="VMZ53_21255"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HUS31058.1"
                     /translation="MRYLITGCFGFVGAHLVRHVRSVDAAAEIVALDRRPAPAGFPEV
                     TRSYCLDLLDDAALSEVFAREQPDRLVHLASFSSVGFSWENPVESFKNNTNIFLAVLE
                     QVRRCSRHTRVLSVGSSEEYGRVVVDELPLRETSPLRPLSPYAVARVAQEHLGDVYVR
                     GFGLDIVSTRSFNHVGPGQSPQFVVSALAKQFAELAKGARTQLAVGTTSVVRDFLDVR
                     DVVAAYCALLDRGTRGEVYNICSGRGTSIANVIDMLASIAEVTPTIVVDQALLRPVEN
                     EIVVGSHDKITAAIGWRPTHTLESSLRDVYADWIARA"
     gene            complement(2844..>3987)
                     /locus_tag="VMZ53_21260"
     CDS             complement(2844..>3987)
                     /locus_tag="VMZ53_21260"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HUS31059.1"
                     /translation="MRIGINALFLIPGKVGGSETYVRSLVRALQAVDDRNDYVLYTNV
                     ENAGSFDLTSPRWREVRAPIKATSRVARMGWEQAVLPLQTIRDRIDVLHSPGYTAPLA
                     LRCANLASILDLNYHFHPEDWTPAALRVNRALIPLVAKAATRILTISESSRGAIIDVL
                     GIAPDKVSVSLLGADGNLVDAGPDPAGVVKEKFGLDGPYLFTVTASHPHKNVDGLFRA
                     YELACRDWPSPPPLVVVGIRGRQQERLEAMVRDWKGGGRIVFPGWVDAPTLAALYRQA
                     RVFVFASKYEGFGIPPLEAMSVDVPVVSSNRTSLAEVCGDGAITLDPDDLALFAKEIR
                     RVFDDETLRADLIARGRAQVAKFTWRRTAEVTIAEYERTVREKRAS"
BASE COUNT          640 a         1286 c         1348 g          713 t
ORIGIN      
        1 gccgagcttt tcgcgggctt tgctcgcgtc gccgtggagg tgatcgactt ccgtcgggcg
       61 gaggtagcga gggtcgagct cgacgtactt ctgccagtcg aggccgagcg tgccgaatgc
      121 gacgtcgagg cagtcgcgga ccgagtagga ctcgcccgtg gcgatcacgt agtcgtcggg
      181 cttgtcttgc tggagcatca gccacatcgc ttcgacgtag tcgccggcga agccccagtc
      241 gcgcttggcg tcgaggttgc cgaggaacag cttcttgtcg aggccgtgct tgatgcgggc
      301 agcggcgcgc gtgatcttgc gcgtgacgaa cgggatgccg cggcgtggcg actcgtggtt
      361 gaagaggatg ccgcagcaga tgaacatgcc gtacgcgtcg cggtagttct ggcagagctg
      421 atgcgcgaag actttggcgc aggcgtaggg gctgcgtggc tcgaacttcg tgtcctcgtt
      481 ctgcggcggt ttcgcgctgc cgaacatctc gctcgacgac gcttggtaga acttcgcctt
      541 gacgtccatc tcgcgcaccg cttcgagcag gcgcagcgtg cccatcgcga ccgtcgcgca
      601 ggtgtactcg ggaacgtcga agctgacgcg cacgtgtgat tgggcggcga ggttgtagat
      661 ctcgtcgggc tggatcttct tgacgaggtt tgcgagcgac gcgccgtcct cgagatcgcc
      721 gtagacgagg cgcaggcggt agtcctgcac gtgcggatct tggtagatgc cttcgagccg
      781 ctcggtgttg aacgagctcg agcggcgaac aacgccgtag acctcgtagc ctttcgcgag
      841 cagcagctcg gccaggtacg aaccatcttg gccggtcacg cccgtgatca gtgctttctt
      901 catcggttct ccaagtacca agcgacggtc tgctcgaggc cagcggcgaa cgaggtcttt
      961 gcgacgaagc cgaagcgttc cttggctctc gacacgtcga gccggcggcg cggctggccg
     1021 tttggttgcg cggtatccca gacgaacgtg ccggtgaagc cggtcgcctt ggcgacgagc
     1081 gtcgcgagat ccttgatcga gatttcctcg ccactgccga tgttgaccgg gtcgctcgag
     1141 tcgtagcgct cggcggcagt gacgaacgct tcggcggcgt cgggtgcgta gatgaactcg
     1201 cgcgttggcg agccgtcgcc ccacagcgtg acgcttgtct tgccttcgtc gcgcgccgag
     1261 aggaacttgc gaatcatcgc gggaatcacg tgcgacgtgc gcagatcgaa gttgtcgcgc
     1321 gggccgtaca tgttgacggg gaacagcacg atgctgttca tgccgtactg ctcgcggtac
     1381 gcctgcgact gcacgagcag cagcttcttc gcgacgccgt acggcgcgtt cgtctcctcg
     1441 ggatagccgt tccagatgtc gtcttcgcga aacggaatcg gcgcgaactt gggataggcg
     1501 cagatcgttc cggagatgac gagcttggga atcttcgcga gccggcattc ttcgatgagc
     1561 tgcaggccca tcatcgcgtt gtcgaagatc atgccgccgg ggttgtcgcg attcgcgccg
     1621 atgccgccga cgcgtgcggc caggtgaaac acgacatcgg gccgcgagtc ggcgagggcg
     1681 cgcttgcagg ccgcgcggtc gaccaggttg tactcgctgc tccgcggcac gaacacatcg
     1741 gtcacacctc ggcgcttcag ctcgtcgacc acgaagctgc cgaagaagcc cgcgccgccc
     1801 gtgacgagca cgcgcttctg cgaccaagaa aaagagctca cgccgtcgac gctattacat
     1861 cagtttccag ggtttggcca gcgagacagc tataggatgc ggcccgtgcg gtatctgatc
     1921 acaggctgct tcgggttcgt cggagcccac ctcgtgcggc acgtccgcag cgtggatgcg
     1981 gccgccgaga tcgtggcgct cgatcggcgt cccgcacccg ctgggtttcc ggaggtcacg
     2041 cgcagctact gcctggatct cctcgacgat gcggcgctct ccgaggtgtt cgcgcgcgag
     2101 cagcccgacc gcctcgttca cctcgcgtcg ttcagctccg tcggcttctc gtgggagaac
     2161 cccgtcgaga gcttcaagaa caatacgaac atcttcctcg ccgtgctcga gcaagtgcgg
     2221 cgctgctcgc gtcacacgcg cgtgttgtcc gtcggctcct ccgaggaata cggacgcgtt
     2281 gtcgtggacg agctgccgct gcgcgagacg agcccgctgc ggccactgag cccgtacgct
     2341 gtcgcgcgcg tcgcgcagga gcacctcggc gacgtctatg tccgcggctt cggcctcgat
     2401 atcgtctcga cgcgctcgtt caaccacgtc ggcccggggc agagcccgca gttcgtcgtg
     2461 agcgcgctcg cgaagcagtt tgccgagctc gcgaaaggcg cgcgcacgca gctcgccgtc
     2521 ggcacgacgt ccgtcgttcg cgacttcctc gatgtccgcg acgtggttgc ggcgtactgc
     2581 gcgctgctcg atcgcggcac gcgcggcgag gtctacaaca tctgcagcgg ccgcggcacg
     2641 agcatcgcga acgtgatcga catgctcgcg tcgatcgccg aggtcacgcc gacgatcgtc
     2701 gtcgaccaag cgctgttgcg ccccgtcgag aacgagatcg tcgtcggttc gcacgacaag
     2761 atcacagcgg cgatcggctg gcgcccgacg cacacactcg aatcatcgct gcgcgacgtc
     2821 tacgccgact ggatcgctcg cgcttagctc gcgcgtttct cgcggacggt gcgctcgtac
     2881 tcggcgatcg tgacctcggc cgtgcggcgc cacgtgaact tcgcgacctg tgcgcggcca
     2941 cgtgcgatca gatctgcgcg cagcgtttca tcgtcgaaca cgcgccggat ctctttcgcg
     3001 aacagcgcga ggtcatcggg atcgagcgtg atcgcgccat cgccgcagac ctcggcgagc
     3061 gacgtccggt tcgacgacac gacgggcacg tcgacggaca tcgcctcgag cggcgggatg
     3121 ccaaagccct cgtacttcga cgcgaacacg aacacgcgcg cttgccgata gagcgcggcg
     3181 agcgtcggtg cgtcgaccca gccggggaac acgatgcggc cgccgccctt ccaatcgcgc
     3241 accatcgcct cgagccgctc ctgctgccgg ccgcgaatgc cgacgacgac gagcggcggc
     3301 gggctcggcc agtctcgaca cgcgagctcg tacgcgcgga acagcccatc gacattcttg
     3361 tgcggatgcg acgcggtgac cgtgaagaga tacggcccgt cgagaccgaa cttctccttc
     3421 acgacgcccg ccggatccgg ccccgcgtca acgaggtttc cgtcggcacc gagcagtgac
     3481 acgctgactt tgtcgggcgc gatgccgagc acgtcgatga tcgcgccgcg cgacgactcc
     3541 gagatcgtga ggatgcgcgt ggcggccttc gcgacgagcg ggatgagcgc acggttcacg
     3601 cggagcgcgg cgggcgtcca gtcctcggga tgaaagtgat agttgagatc gaggatcgac
     3661 gcgaggttcg cgcagcgcag cgcgagcggc gcggtgtagc ccggcgagtg cagcacgtcg
     3721 atgcggtcgc ggatcgtctg cagcggcagc accgcctgct cccagcccat acgtgcgacg
     3781 cggctcgtcg ctttgatcgg agcgcgcact tcgcgccagc gcgggctcgt gaggtcgaag
     3841 ctgcctgcat tctcgacgtt cgtgtagagg acgtagtcgt tgcgatcgtc gacggcctgc
     3901 agcgcgcgaa cgagcgagcg cacataggtc tccgagccgc cgaccttgcc ggggatcaag
     3961 aacagcgcat tgatcccgat gcgcatc
//
LOCUS       DATLBZ010000419        16807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4981829, whole genome shotgun sequence.
ACCESSION   DATLBZ010000419 DATLBZ010000000
VERSION     DATLBZ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16807
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4981829"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VMZ53_21265"
     CDS             complement(<1..689)
                     /locus_tag="VMZ53_21265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31060.1"
                     /translation="MRTIGGRYAIEGQIGEGGMGLVYRVRHTQLAKHFALKVIAPAFA
                     TDAKARERFNVEAQLASEISHPNIVSVVDFGEDDELGAYMVMELVEGEPFLFAEGSSP
                     LSVRRALDVLAQVADALDHIHKRGVIHGDIKAENLILAEEPVAAGTRRRRIVRLLDFG
                     LAKRFGTQDTELNGTPHYLAPERCTGGVASVASDIYALGVLGYLLLTRTMPFDGNLAQ
                     ILDAHVREAPP"
     gene            complement(704..1222)
                     /locus_tag="VMZ53_21270"
     CDS             complement(704..1222)
                     /locus_tag="VMZ53_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31061.1"
                     /translation="MTYAILLGVLAVFDFTLAGFRSAAGRDGRIRKQPLFRAALTRGV
                     VFGVVVVGVHAAIAGVLVATGDATTWPAFVDAGRDAVIVFGVFATATALALAFWFAPA
                     QELRLVPTLIVLGPLTMLRPVVIAGGLAWAAVRSSEPRVWLVAILAGVSMLAAERVLG
                     VPYRDRWKRLVS"
     gene            complement(1219..2157)
                     /locus_tag="VMZ53_21275"
     CDS             complement(1219..2157)
                     /locus_tag="VMZ53_21275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS31062.1"
                     /translation="MDDQFGPEVSDVIVDYDASTLARRDVLKDRALAALAQSPAIARR
                     LARNLSATADGHIVRSEVDGILIRSHLELQRLHEEFRIAPMMRDLLRPLIALAREATG
                     RKRIRIVDLGCGMGFIVRWLAANGELGADRGDVELIGADYNLALLEAAKRLATDEMLP
                     VRFVAANAFELDDAPADIVMSTGVLHHFRGTSLDDLFANHERSSAVAFLHADIRPSVV
                     APPGSWVFHRARMREPLAKFDGYWSAVRAHDAQTLRKSIASSAPGFALGLVDAKITSV
                     LALLRIFQVAVAVRRPHVASLAQSYKHLGRRFEVVA"
     gene            complement(2342..2845)
                     /locus_tag="VMZ53_21280"
     CDS             complement(2342..2845)
                     /locus_tag="VMZ53_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HUS31063.1"
                     /translation="MNKQQFAVGDKAVYPVHGVAEVVALEQRDVGGNKIPVYILKILD
                     TGMKIMVPTTNAGSVGLRVLIHSKQVKEVYEILKERDIPRDTQTWNRRYREYMEKIKT
                     GSVFEIAEVLRDLCVLRMTKDLSFGERKMLDTARTLLVKELALAKGVGDEKIAGEIDA
                     LFQPAAA"
     gene            complement(2956..4470)
                     /locus_tag="VMZ53_21285"
     CDS             complement(2956..4470)
                     /locus_tag="VMZ53_21285"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HUS31064.1"
                     /translation="MTLLTGPALSAQVDVLALTTFGDPTKDGVFKSADQALGGHLADV
                     AKQESFDGKTGQVVSVHTHGKLGAKRVVIVGAGSRGDFTNSSIRDLTAAVAQYANKVN
                     ATSVAFVLPALGAGREAQIVQLAAEGVHLGGYRFGRYLTGDDAKKPTSLKTFQLSTDA
                     KGKKLGASQARTLEGAIARGTAIATATNLARDLINEPAAVATPSYIAQQAQALAKKHK
                     GSLTVTVLDGKKCAELGMGMFLAVGQGSDQEPRFVHITYKPSKKPKKKVCFIGKGVTF
                     DSGGYSLKPSASMEDMKIDMSGAAAVIGAMDAIATLGSEYEVHAVSALCENLVSGRAY
                     KLGDVLKSMDGQTVEINNTDAEGRLTLGDAITYARQKIDPDELFDFATLTGACMVALG
                     PYTAGVFGDNEPLVKNWLAAAERTGEDMWRLPLNSRLREQLKSPIADMRNTGDRYGGA
                     ITAALFLKSFAKDTPWVHVDIAGPASTSSNRPSQPKGGTGFAVATIVEYATAKR"
     gene            4635..4832
                     /locus_tag="VMZ53_21290"
     CDS             4635..4832
                     /locus_tag="VMZ53_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31065.1"
                     /translation="MRSKGMTARPAARKGDGNRTKKTVAVPSRKSQPIIQMVERKSSP
                     NITELPKKVGELPIPTATFVF"
     gene            complement(4840..5598)
                     /locus_tag="VMZ53_21295"
     CDS             complement(4840..5598)
                     /locus_tag="VMZ53_21295"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUS31066.1"
                     /translation="MLTDATDKTLRVGTVDVPARIDRERYFRELTYVELSALFAGPQK
                     PSTIAKWAELSEPGSIGLVAPFVFTHRKPPAGQKLWPHDSTTGDFRSSALAREALEPL
                     KETVKAVSAGCVVFRSPESFSASAANRDQLKQFFSEVATEDAIGCSRVWVPGGLWEVR
                     TAVKLAEELGVTCAIDPMVQEPGATTDPYVELEATSLYLRIEGTGRAGLIRNERLEDL
                     AALIESYEDLPLTVAFNSPERWQDARNFVKLLNG"
     gene            5668..6312
                     /gene="fsa"
                     /locus_tag="VMZ53_21300"
     CDS             5668..6312
                     /gene="fsa"
                     /locus_tag="VMZ53_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HUS31067.1"
                     /translation="MKFFIDTADVKEIREAAAMGLVDGVTTNPSLVAKTGKKFRDVLL
                     EICDIVKGPVSAEVVSTKHDDMMKEAREYAALRSNIVVKIPLIPEGLKAVKTCTDEGI
                     KTNVTLCFSATQALLAAKAGATYISPFVGRLDDISTDGMQLIGEILEIYQNYNFQTEV
                     LVASVRSPMHVHQAARMGAHVSTCPLSVLLQLSKHPLTDVGLAKFLSDWEKVPK"
     gene            6362..6793
                     /locus_tag="VMZ53_21305"
     CDS             6362..6793
                     /locus_tag="VMZ53_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HUS31068.1"
                     /translation="MAPARRAFSVAVYARKGERVLVIEHKRLKTWLPIGGELEAGETP
                     LEAAVRELREETGLAGTFHTLSGALDGVPSGLIGYEEHQAGSKGLHMNFVFVCEVRPD
                     SEVTPNDEFGAYRWVNRAELEQLESPLNVRQFGYVALDARW"
     gene            6891..7493
                     /locus_tag="VMZ53_21310"
     CDS             6891..7493
                     /locus_tag="VMZ53_21310"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31069.1"
                     /translation="MRVRQHVNPLDVFFETFRGTVPALRPGCPVEVEIGCADAQFLFE
                     RAAQDPSRDYVGLEIREDLVEYVAKKASKTGLHVQAVWCHAQLHMKQIFAAGVASRVY
                     VNFPDPWFKRRHHVRRMVDDVLAAQIDYLLAPDGELFVQTDVWDIALDALSAFEPRFV
                     NRAGEWTFWKDGNPYGVRSWREQNAEETGLPVWRLLYRRY"
     gene            complement(7487..8125)
                     /locus_tag="VMZ53_21315"
     CDS             complement(7487..8125)
                     /locus_tag="VMZ53_21315"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HUS31070.1"
                     /translation="MRKKSRLFAIAEMLRARRTGVTAAQIAERFNVTLRTVYRDLQAL
                     QDAGMPIKADRGRGGGYALDTNYQLPPINFTAREAALLVALARMASEQRLMPFPSTLE
                     RAADKVRAALTTSAQRELLSLIKDLQIVGVPSLPIPASVREAIETAWFESRALRIVYE
                     KKAWQLSPRIVKIRNLVFDRQVTLLNVVDLETNQDRQFRLDKIKQATVLDTQ"
     gene            8188..8652
                     /locus_tag="VMZ53_21320"
     CDS             8188..8652
                     /locus_tag="VMZ53_21320"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS31071.1"
                     /translation="MTIDMNKKLSRPAGHHTVTPGFSVQGSQKTLEFLQKAFGAEVVD
                     KYEMPNGSLAHCEVRIGDSVVMFGEAMPDHGMPAMPASLSLYVENGNAVDATFKRAVA
                     AGGTSVMEPANQFYGYRSATLRDPGGNKWTICAVVEELTKAEIEQRMASQKG"
     gene            8740..10815
                     /gene="uvrB"
                     /locus_tag="VMZ53_21325"
     CDS             8740..10815
                     /gene="uvrB"
                     /locus_tag="VMZ53_21325"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HUS31072.1"
                     /translation="MTTYRGKKARRSNESSPPPPLPERKGAGFRLKSPYEPAGDQPKA
                     IRELVDGLKRGDEAQVLLGITGSGKTYSVANVIAEVNRPTLIIAHNKILAAQLYGEFK
                     ELFPENAVHYFVSYYDYYQPEAYVPTTDTFIEKDSIVNEEIDRMRHAATFALLSRRDV
                     IIVASVSCIYGIGERDTYATMTCELDVGTSIDRDAVLRRLVELQYERNDVDFHRGTFR
                     VRGDTVEVFPAYEADTAIRIEWWGDQIEAMNEIDPLRGGIKRKLDRSVIFAASHYATP
                     ADTMRRACESIRQELRGRLEELSQQGKLLERQRLEQRTMYDLESIEQMGFCSGIENYS
                     RHLTGRSAGEPPPTLMDYFHDDYLLIVDESHQTVPQIGAMYNGDRSRKETLVEFGFRL
                     PSALDNRPLRFDEWRQRAKQAIYVSATPAEWELNEAKGVVVEQIIRPTGLLDPEVEVR
                     PVAHQVDDLLAEIRERTAKGDRVLVTTLTKRMAEDLTEYYREIGIRVRYLHSDVDTLE
                     RIQIIRDLRRGEFDVLVGINLLREGLDIPEVSLVGILDADKEGFLRSERSLIQTIGRA
                     ARNLHGKVLLYADKETDSIRDAVGETRRRRTVQDQYNKEHGITPRSASRAIMEMQVAE
                     PLPKKGQRSYQLAAVDLKKIDDIDSLRAAIDKLRTEMRQAAADLEFEKAAALRDKARE
                     LEQLELQMR"
     gene            10859..11458
                     /gene="thiE"
                     /locus_tag="VMZ53_21330"
     CDS             10859..11458
                     /gene="thiE"
                     /locus_tag="VMZ53_21330"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HUS31073.1"
                     /translation="MFLSGFYAVLDRDDEALARMLVGPGGAKILQVRIKPGEARDVVK
                     VAKMARKVCDEAGALLVINDRIDIALAVRADAVHLGQTDLSLPDARSIAGDRLAIGVS
                     THNLHQVEAAVAQRPDYIAYGPIFETTTKQNPDPVQGLDALRVAVEAAGTSPVVAIGG
                     ITPAHAADVYAAGAAAICAISAVNNAADVVAAARAFARV"
     gene            11468..13801
                     /locus_tag="VMZ53_21335"
     CDS             11468..13801
                     /locus_tag="VMZ53_21335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31074.1"
                     /translation="MCPDDDDLGSAKTAIGGTMPSQPGVSRVVAEPVTSVIADRYEVQ
                     ALLGMGGMGRVYRALDRTLDDVVALKMLRRELLDTPGVLERFRQEVKLARRVTHANVV
                     RTFDLGQHGQDHYLTMEYIEGRSLAQLIDDGPLAFDEIMRIARAVAAGIAAAHANGVL
                     HRDLKPDNVLIAKTGRIAITDFGIATASTSPTVTSDRLVGTPAYMAPEQLEEGATIAA
                     PADVYAFGTILFEMATGRRPFVGNDPIQVALARLHTPPPDPRTLRQVPEGLAELCIAC
                     MGRRPETRPADGSGLVAALASLSDLSSRPRTGRLAMDTPTRTSRSVAILPLRGTGDLG
                     DVAEGLSEEIVDALSMTRSLRVRPLASVRKAVRADSDARDVGQQLGVDVVVEGSVRRR
                     GDHVRITARMVSVADGFQLWASHIDTGTDGLLAASDDLVEAIAKALTVDLAMPDRVMV
                     DPEASSLYLEGKAKVRLNWIDGNMEPAIESLQQALELAPNDPAILATLSQALARSAFY
                     GAPASRIAQARELAERAVELAPRMGVPHIAVGTASLYDGDAPASVAAFARALQHSPGS
                     AMAQAMLGAALLEAGALDDAIARLEVSRSLDPLGLAHIDLPRAYFYAGRWDDTVAEYE
                     RARVAKDFDLRLFDDIMFARLKLWRGELAPIAEVPGTSTPPNVFAYAEVVRRIHATGV
                     FSAADRAAMAAIVDIDNRRLRASRAQFMAEYLMFAGEPDEALRYVRIGVEAGLQDYLW
                     AQKCPLLAPLRARAEFQALEATVAERSRAVIAASRAA"
     gene            complement(13802..14131)
                     /locus_tag="VMZ53_21340"
     CDS             complement(13802..14131)
                     /locus_tag="VMZ53_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31075.1"
                     /translation="MAKLAIFASIATIVASRGIASLTASDAQAVTACVTKEFKTQLAK
                     DACAKGGQPAAKDAMKKFMKDAKIKSCNQCHSKLAPKYELKADGVDQFKKAGGTLVTG
                     AAAPAKK"
     gene            14224..14736
                     /locus_tag="VMZ53_21345"
     CDS             14224..14736
                     /locus_tag="VMZ53_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31076.1"
                     /translation="MKRTVPILLLLCGSASADTLTLAPVPSASEPNVVGSKAVCKGDN
                     DFLRVARTKLDGHEVLVLGDCAGTHSYLALETKSGWTRFVSAPGWGSVTSTKLKKRDV
                     LLHRIDTYVDQDKSTSRVDLCVYDDKAGTPSCGSAEVECPETGCREPEIIKGALWMHA
                     AGGRKRFLID"
     gene            complement(14747..15469)
                     /locus_tag="VMZ53_21350"
     CDS             complement(14747..15469)
                     /locus_tag="VMZ53_21350"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31077.1"
                     /translation="MRRDITALQGRVDTKEKELDTQIKQLQAVLDEATKLLKRNSADI
                     GADVEKLAADIRTANGLTAAINNSINELKVGFDKYRTENNARMDSLEQRVAQIESGKP
                     TANSGPDELWKLGTTAFEAARYNDALDIYKRLVQTFPTHARADDAQYFRGQAYTNLKD
                     FDNAIAAYQQLVQKYPDSDLADDGLYFAALAAQSLKNCTEARTYAGLVKSKYPKSNVL
                     KQAGDLDATLKKDSKNKAKCSS"
     gene            complement(15556..16299)
                     /locus_tag="VMZ53_21355"
     CDS             complement(15556..16299)
                     /locus_tag="VMZ53_21355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS31078.1"
                     /translation="MGKVFVAIAVITLALAAGCGSKPKPPGCKGDNDCKKPLVCATNK
                     CVECRDDSQCPKGKKCSGNACVVKPACITDDQCPSGQVCQAGSCKPCAGDGECGPGGK
                     CEAGACQRGKKCTKDEECADDEDCINGYCVRAGAGSNVDAGCTLNTVYFAFDDSTIQA
                     SERDRLDKNNACMSTNKDKAVLVIGHTDTSGTEEYNIALSERRAQQVADYLARLGSDP
                     AKLQVVPKGETEPTGNGDDKDRRVEFKWR"
     gene            16357..>16807
                     /locus_tag="VMZ53_21360"
     CDS             16357..>16807
                     /locus_tag="VMZ53_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS31079.1"
                     /translation="MARSTILVIDDEPNIPTTVRRSLELEGYSVEVAGGGAAGLAKLA
                     EHDIDMVLLDVMMPGETGLEVLPKIRTMKPETMVVMMSGNATIETAVHATKAGAYDFI
                     EKPLSGDKLLITVQNTLAFKRLRYENQRLRGRVQTDFAMIGKGAAMKA"
BASE COUNT         2965 a         5360 c         5440 g         3042 t
ORIGIN      
        1 ctcggtggtg cttcccgcac gtgcgcgtcg aggatctgtg caaggtttcc gtcgaacggc
       61 atcgtgcgtg tgagcaagag atatcccagc acgccgagcg cgtagatgtc gctcgcgacg
      121 ctcgcaacgc cgccggtgca gcgctcgggc gcgaggtaat gaggcgtgcc attgagctcg
      181 gtatcctgcg taccgaagcg tttcgcgaga ccgaagtcga gcaggcgaac gatgcgtcgt
      241 cgccgcgtgc ctgcggccac tggctcctcg gcgaggatga ggttctccgc cttgatgtcg
      301 ccatgaatga cgccacgctt gtggatgtga tcgagtgcgt cggccacttg cgcgaggaca
      361 tcgagagcgc gccgcacgct cagcggtgac gagccctctg caaacaagaa tggctcgcct
      421 tcgacgagct ccatcaccat gtatgcgccg agctcgtcgt cctcaccgaa gtcgacgacc
      481 gacacgatgt tcggatgcga aatctcgctc gcgagctgcg cctcgacgtt gaagcgctct
      541 ctcgccttcg cgtcggtcgc aaacgcaggc gcgatcacct tgagcgcgaa gtgcttcgcg
      601 agctgcgtat gacgcacgcg atagacgagg cccatgccgc cttcgcctat ctgcccctca
      661 atcgcgtacc gacccccgat cgtccgcacc aacaccgatt gtactacgac accaatcgct
      721 tccagcggtc tcgatacgga acaccaagca cgcgctcggc cgccagcatc gacactcccg
      781 caagaattgc aacgagccac acgcgcggct cgctcgatcg cacagctgcc cacgccagcc
      841 cacctgcaat cacgacgggg cgcagcatgg tgagcggtcc caagacgatc aacgtcggca
      901 cgaggcgcag ctcttgcgcg ggcgcgaacc aaaacgccaa cgcaagcgcg gtcgccgtgg
      961 cgaagacgcc gaacacgatc accgcatcgc ggccggcgtc gacgaatgct ggccacgtcg
     1021 tcgcgtcacc ggtcgctacg aggacacctg caatcgccgc atgtacgccg accacgacga
     1081 cgccgaacac aacgccacgt gtgagagccg cgcgaaacag cggttgcttg cgaatgcggc
     1141 catcgcgccc agctgccgag cgaaagccgg cgagcgtgaa gtcgaagacg gcgagcacgc
     1201 cgagaagaat cgcgtaggtc acgcgacaac ctcgaagcgg cggcccaggt gcttgtagct
     1261 ttgcgcgagt gacgcgacat gcggccgcct tacggcaacg gcgacttgga aaatgcgcag
     1321 gagcgcgagc acgctcgtga tcttcgcgtc gacgaggccg agcgcgaagc cgggtgcact
     1381 gctcgcgatc gacttgcgga gtgtctgcgc atcgtgcgcc cgcactgccg accaatagcc
     1441 gtcgaacttc gcgagaggct cgcgcatacg cgcgcggtga aagacccacg agcccggcgg
     1501 cgcgacgacg cttggccgga tgtcagcatg caagaacgcg acggcgctcg agcgctcgtg
     1561 gttcgcgaag aggtcgtcga ggctagtccc gcgaaagtgg tgcagcacgc cggtcgacat
     1621 gacgatgtcc gctggtgcgt cgtcgagctc gaacgcgttg gccgcgacaa agcgaacggg
     1681 cagcatttcg tcggtcgcga gcctcttcgc ggcctcgagc aacgcgaggt tgtagtccgc
     1741 gccgatgagc tcgacgtccc cgcggtccgc gccgagctca ccattggcgg cgagccaccg
     1801 cacgataaag cccatcccgc atccgaggtc gacgatgcga atgcgtttgc ggccggtcgc
     1861 ctcacgcgcc aacgcgataa gcggtcgcag aaggtctcgc atcatcgggg cgatgcggaa
     1921 ctcttcgtgc agccgctgga gctcgaggtg cgagcggatc agaatgccgt cgacttcgct
     1981 gcgaacgata tggccatctg ctgtagcaga caggttccgc gcaaggcgcc gagcgatggc
     2041 tgggctctgc gcgagcgcgg cgagtgcgcg atctttcaag acatcgcggc gagcgagcgt
     2101 cgacgcatca taatcaacga tgacgtccga cacctcaggc ccgaactgat cgtccacgcc
     2161 acgaagctat cgcggatggc cgcccaccgg acgtgctccg tccctcgcgg ttgcggcggt
     2221 tgcggcggtt gcggaagttg cgtccgtccc ttagcggtca gaccgatcgg ctcgcatggc
     2281 cgcccgccgg acgtgctccg tccctcgcgg ttgcggcggt tgcggaagtt gcgtccgtcc
     2341 cttaggcggc ggccggctgg aagagggcgt cgatctcgcc cgcgatcttt tcatcgccca
     2401 cgcccttagc gagcgcgagc tctttgacga gcaacgtccg ggcggtgtcg agcatcttgc
     2461 gctcgccgaa cgacaggtct ttcgtcatgc gaagaacgca gaggtcgcga agcacttcag
     2521 cgatctcgaa gacggagccc gtcttgatct tctccatgta ttcgcgatag cggcgattcc
     2581 aggtctgcgt gtcgcgcggg atgtcccgct ctttgaggat ttcgtagacc tctttgacct
     2641 gcttggagtg gatcaggacg cgaaggccca cggagccggc gttcgtcgtc ggcaccatga
     2701 tcttcatccc ggtatcgagg atcttgagga tgtagacggg gatcttgttg ccgccgacgt
     2761 cccgctgctc caacgccacc acctctgcaa cgccatggac gggatagaca gccttgtccc
     2821 ctactgcgaa ttgctgcttg ttcaagactt cgaaatggta gcagcggtgg ggctaactgt
     2881 caaattacgc accaagttaa gcgcgtgtaa ttgtttgaca aagaagaaat ccgagcgtga
     2941 tttgatcacg cgaaatcagc gttttgcagt cgcgtattcg acgatcgttg cgaccgcgaa
     3001 gccggtcccg cctttgggct gagaggggcg gttgctcgag gtcgaggccg gcccggcgat
     3061 gtcgacgtgg acccacggcg tgtctttggc gaacgacttg aggaagaggg ctgcggtgat
     3121 cgcgccgccg tagcgatcgc ctgtgttgcg catgtcagcg atcgggctct tgagctgctc
     3181 gcgcaggcgc gagttgagcg gcaggcgcca catgtcttcg cccgtgcgct ccgcagcggc
     3241 gagccagttc tttacgagcg gctcgttgtc gccgaacacg ccggccgtgt acgggccgag
     3301 ggcgaccatg caggcgccgg tcagagttgc gaagtcgaag agctcgtcgg gatcgatctt
     3361 ctggcgggcg taggtgatcg cgtcgccgag agtgaggcga ccttctgcgt ccgtgttgtt
     3421 gatctcgacc gtctggccgt ccatcgactt gaggacgtcg ccgagcttgt acgcgcggcc
     3481 cgagacgagg ttctcgcaga gcgcgctgac ggcgtgcact tcgtattcgg agccgagcgt
     3541 cgcgatcgcg tccatcgcgc cgatgacagc ggccgcgccc gacatgtcga tcttcatgtc
     3601 ttccatcgac gcgctcggct tgagcgagta gccgcccgag tcgaacgtca cgcccttgcc
     3661 gatgaagcag actttcttct ttggcttctt cgacggcttg tacgtgatgt ggacgaagcg
     3721 cggctcttga tcgctgcctt ggccgacggc gaggaacatg cccataccga gctcagcgca
     3781 cttcttgccg tcgagaacgg tgacggtgag cgagcctttg tgcttcttcg cgagcgcttg
     3841 cgcttgctgc gcgatgtacg agggcgttgc gacggcggcg ggctcgttga tcaaatcgcg
     3901 cgcgaggttc gttgccgtgg cgatcgcagt gccacgtgcg atggcgcctt cgagcgtgcg
     3961 cgcttgcgat gcgccgagct tcttgccctt cgcgtccgtc gagagctgga acgtcttcag
     4021 gctcgtcggc tttttcgcgt cgtcacccgt gaggtagcga ccgaagcggt agccaccgag
     4081 gtgaacgcct tcggctgcga gctgaacgat ctgagcttcg cgaccagcgc cgagcgcggg
     4141 caggacgaac gcgacgctcg ttgcgttgac cttgttcgcg tactgcgcga cggccgcggt
     4201 gagatcgcga atcgaagaat tggtgaagtc accacgcgag cctgcaccga caatgacgac
     4261 gcgctttgcg ccgagcttgc cgtgcgtgtg gacgctgacg acctgaccgg tcttgccgtc
     4321 gaacgactct tgcttcgcga cgtccgcgag atggccaccg agcgcctggt ctgctgattt
     4381 gaaaacgccg tccttcgtgg gatcgccgaa ggtcgtgagt gccagtacat cgacctgagc
     4441 cgagagggcc ggacccgtga gaagcgtcac tgcagtggct gcgttcgtgt ccataaacgg
     4501 ctgtaactcc ctagaaaagc gctggcgtta tacgagcgac agtgaccgaa gtaaaggaat
     4561 cagattgcct acattgactt gctatgaggc agatccgtag agtcttgaac ggacagatcc
     4621 ctgggggatt ttgaatgcga agcaagggaa tgacggcgcg tcctgcggcg cgcaagggtg
     4681 acggcaaccg cacgaagaag accgttgcgg ttccgtcgag gaaatctcag ccgattatcc
     4741 agatggtgga gcggaagtcc tcgcccaaca tcacggagct cccaaagaaa gtgggggagc
     4801 tgccaatccc gactgccacg ttcgtcttct aagctccgct caaccattta gtaacttcac
     4861 gaagtttcgc gcgtcttgcc agcgctctgg tgagttgaac gcgaccgtga gcgggaggtc
     4921 ctcgtagctc tcgatgagag ccgcgaggtc ttccagccgc tcattccgga tcaacccggc
     4981 acgcccggtg ccttcgatcc gaagatagag tgaggtcgct tcgagctcga cgtatggatc
     5041 cgtcgtcgcg cccggctcct gcaccatcgg atcgatcgcg caggtgacgc cgagctcctc
     5101 cgcgagcttc accgcggtgc ggacttccca cagaccaccg gggacccaca cgcgggagca
     5161 accgatcgca tcttcggtcg cgacctcgct gaagaactgc ttcaactgat cgcggttcgc
     5221 ggcggacgcg gagaacgact cgggcgatcg gaagaccacg caacctgcgg ataccgcctt
     5281 cacggtttcc ttcagcggct cgagcgcctc acgtgcgagc gcgctactcc ggaaatcgcc
     5341 cgtcgtcgaa tcatgcggcc acagcttctg accagccggc ggcttgcgat gcgtgaacac
     5401 aaacggcgcg acgagaccga tcgagccagg ctcggagagc tcggcccact tcgcaatcgt
     5461 gctcggtttc tgcggaccgg caaagagcgc ggacagctcg acgtacgtga gctcccgaaa
     5521 gtaacgctct cggtcgatgc gagcaggaac gtcgacggtt cctacgcgca gggtcttgtc
     5581 ggttgcgtct gtcaggatgg cctcgtatgg tcgcgacgac ggcggacgct acgtcgtcgt
     5641 cgagtagggt gcaaccccgg gaggcccatg aagttcttca tcgatacagc cgatgtcaaa
     5701 gagatccgag aggccgccgc gatggggctc gtcgacggtg tgaccacgaa tccatcgctc
     5761 gtcgcgaaga ccggcaagaa gttccgcgac gtcctcctcg agatctgcga catcgtcaag
     5821 gggcccgtct ccgccgaggt cgtcagcacg aagcacgacg acatgatgaa ggaagcgcgc
     5881 gagtacgccg ctctccgctc gaacatcgtc gtgaaaattc cgctgatccc cgagggtctc
     5941 aaagcggtga agacctgcac cgacgaaggc atcaagacca acgtcacgct gtgcttctct
     6001 gcgacgcagg cgctgctcgc cgcgaaggcg ggcgcaacgt acatctcgcc gttcgtcggc
     6061 cgtctcgatg acatctcgac cgacggcatg cagctcatcg gtgagatcct cgagatctac
     6121 cagaactaca acttccagac cgaagtgctc gtcgcgtcgg tgcgcagccc gatgcatgtg
     6181 caccaagccg cgcgcatggg agcgcatgtt tcgacgtgcc cgctctcggt gctgctgcag
     6241 ctgtcgaagc atccgctgac ggacgtcggc ctcgcgaagt tcctctcgga ctgggaaaaa
     6301 gttccgaagt gagcgcgagg gatcgccgcg cggcagatgt gatcggaccg agcgcgtgat
     6361 catggctcct gctcgtcgcg cgttctccgt cgcggtctac gcacgcaaag gcgagcgcgt
     6421 cctcgtcatc gagcacaagc gtctgaagac gtggctgccg atcggcggcg agctcgaagc
     6481 gggcgagaca ccactcgaag ctgcagtccg cgagctgcgc gaagagacgg gcctcgccgg
     6541 cacatttcat acgctgtccg gcgctctgga cggcgttccg tccggcctca tcggctacga
     6601 agagcaccaa gccggcagca aaggtctgca catgaacttc gtcttcgtgt gcgaagtccg
     6661 gccggacagc gaagtcacgc cgaacgacga attcggtgcg taccgctggg tgaatcgcgc
     6721 cgagctcgag cagctcgagt caccgctcaa cgtccgccag ttcggctacg tcgcgctcga
     6781 cgctcgctgg taaccacgct cgcagctttc gctgcaatgg cgcagctttg acgcgcgtca
     6841 aaaagtaccg gggtaagtga atccggagag tgtggtagac ggatgcgcgc atgcgtgtcc
     6901 ggcagcacgt caatccgctc gacgtcttct tcgagacgtt ccgaggaacc gtgcccgcgc
     6961 tgcgtccggg ctgtccggtg gaagtcgaga tcgggtgcgc cgacgcgcag ttcttgttcg
     7021 agcgtgctgc gcaggatccg agccgcgatt atgtcgggct cgagattcgc gaggacctcg
     7081 tcgagtacgt cgcgaagaag gccagcaaga ccgggctgca tgtccaggct gtgtggtgtc
     7141 acgcgcagct gcacatgaag cagatcttcg cggccggcgt ggcgtctcgc gtgtacgtga
     7201 actttccgga tccgtggttc aagcgccggc atcacgtgcg gaggatggtc gacgacgtgc
     7261 tcgccgcaca gatcgactac ctcctcgcgc ccgacggcga gctgttcgtg cagaccgatg
     7321 tctgggatat cgcgctcgac gcgctcagcg ccttcgagcc ccgcttcgtc aatcgcgcgg
     7381 gcgagtggac gttctggaag gacggcaatc cgtacggcgt gcgttcgtgg cgcgagcaga
     7441 atgccgagga gaccgggctg cccgtctggc gattgctcta tcgacgctac tgagtgtcga
     7501 ggaccgtcgc ttgcttgatc ttgtcgagcc ggaattggcg gtcttggttc gtctccagat
     7561 cgacgacgtt gagcagcgtc acttggcgat cgaacacgag gtttctgatc ttcacgatgc
     7621 gcggcgacag ctgccacgct ttcttttcgt agacgatccg cagcgcacgc gactcgaacc
     7681 acgcggtctc gatcgcttcg cgaacgctcg cgggaatcgg cagcgacggc acgccgacga
     7741 tctgcagatc tttgatcagc gagaggagct cgcgttgcgc ggaggtcgtc agcgcggcgc
     7801 gcactttgtc ggcggcgcgt tcgagcgtcg atggaaacgg catgaggcgc tgctcgctgg
     7861 ccatgcgggc gagcgcgacg agcagtgcgg cctcacgcgc ggtgaagttg atcggcggga
     7921 gctggtagtt cgtgtcgagc gcgtagccac caccgcggcc gcggtcggct ttgatcggca
     7981 tgccggcgtc ctgtaacgcc tggagatcgc ggtagaccgt ccggagcgtc acgttgaacc
     8041 gctccgcaat ctgggccgct gtgacgccgg ttcgacgggc gcgcagcatc tcggcgatcg
     8101 cgaacaggcg gctcttcttt cgcatgaata ctgacaaaag gctgtcacaa gaccctgcca
     8161 aggtcgagac acacaaggag aacaaacatg actatcgata tgaacaagaa gctgtcccgt
     8221 cccgctggcc accacaccgt caccccgggc ttctcggtcc agggctcgca gaagacgctc
     8281 gagtttctgc agaaggcgtt cggcgccgag gtcgtcgaca agtacgagat gccgaacggt
     8341 tcgctcgcac actgcgaagt ccgcatcggc gactccgtcg tgatgttcgg cgaagcgatg
     8401 cccgatcacg gaatgccggc gatgcccgcg tcgctctcgc tgtatgtcga gaacggcaat
     8461 gcagtcgacg cgacgttcaa gcgcgcagtc gcagccggcg gcacgagcgt gatggagcct
     8521 gctaatcagt tctacggcta tcgcagcgcg acgctccgcg atccgggcgg caacaagtgg
     8581 acgatctgcg cagtcgtcga agagctgacg aaggcggaga tcgagcagcg catggctagc
     8641 cagaagggct aagaaccatg acgacacacc aagcatggtg gatgtggctc gctcagctgg
     8701 gccgtgcacg acaacggcgg gcgggctaga gtgccgccgg tgaccacgta tcgcgggaag
     8761 aaggctcggc gaagcaacga gagctcgccg cctccgcctt tgccggaacg gaagggtgca
     8821 ggctttcgcc tgaaatctcc gtacgagccg gcgggtgatc agcccaaggc aatccgcgag
     8881 ctggtcgacg gcctaaagcg tggcgatgaa gcgcaggttc tcctcggcat cacgggctcc
     8941 ggcaagacgt actccgtggc caacgtgatc gccgaggtga accgcccgac gctcatcatc
     9001 gcgcacaaca agatcctggc ggcgcagctg tacggcgagt tcaaggagct gtttcccgag
     9061 aacgccgtcc actacttcgt cagctactac gattactacc agcccgaagc gtacgttccg
     9121 acgaccgaca cgttcatcga gaaggactcg atcgtcaacg aagagatcga tcgcatgcgt
     9181 cacgcggcga cgttcgccct gctgtcgcgg cgagacgtga tcatcgtggc atcggtgtcg
     9241 tgcatctacg gcatcggcga gcgcgacacg tacgcgacga tgacgtgcga gctcgacgtc
     9301 ggaacatcga tcgatcgcga cgcggtgctc cgccggctcg tcgagctgca gtacgagcgc
     9361 aacgacgtcg acttccatcg cggcacgttc cgcgtgcgcg gcgacactgt tgaagtgttc
     9421 ccggcttacg aggctgacac cgccattcgc atcgaatggt ggggcgacca gatcgaagcg
     9481 atgaacgaga tcgatccgct gcgcggcggc atcaagcgca agctcgatcg gtcggtcatc
     9541 ttcgctgcct cgcactacgc gacgccagcc gatacgatgc gccgcgcgtg cgagtcgatt
     9601 cgccaagagc tgcgcggtcg cctcgaagag ctgtcgcaac agggcaagct cctcgagcgt
     9661 caacgtctcg agcagcgaac gatgtacgac ctcgagtcga tcgagcagat gggcttctgc
     9721 tcgggcatcg agaactactc gcgccatctc accggacgct ccgcgggcga accaccgccg
     9781 acgctgatgg actacttcca cgacgactac ttgctgatcg tcgacgagtc gcaccaaacg
     9841 gttccgcaga tcggcgcgat gtacaacggc gatcgctcgc gcaaagagac gctggtcgag
     9901 ttcggcttcc gtctgccgag cgcgctcgac aaccggccgc tccgcttcga cgaatggcgc
     9961 cagcgcgcga agcaagcgat ctacgtatct gccacgcccg cggagtggga gctgaacgag
    10021 gcgaagggcg tcgtcgtcga gcagatcatc cgaccgacgg gcctgctcga tcccgaggtc
    10081 gaagtgcgcc ccgtcgcgca tcaggtcgac gacctgctcg ccgagatccg cgagcgcacg
    10141 gccaagggcg acagggtcct cgtcacgacg ctcacgaagc gaatggctga ggatctgacc
    10201 gagtactacc gagagatcgg catcagggtt cgctacctgc actccgacgt cgacacgctc
    10261 gagcgcatcc agatcatccg cgacctgcga cgtggtgagt tcgacgtcct tgtcggcatc
    10321 aacctactcc gcgaaggcct cgacatcccg gaggtctcac tcgtcggcat tctcgacgcc
    10381 gacaaagagg gattcctccg cagcgagcgc tcgctgatcc agacgatcgg ccgcgccgcg
    10441 cgcaacttgc acggaaaggt cttgctctac gccgacaagg agaccgactc gattcgcgat
    10501 gccgtcggag agacgcgtcg tcgtcgcacg gtccaggacc agtacaacaa ggagcacggc
    10561 attacgccga ggtccgcatc gcgcgcgatc atggagatgc aggttgccga gccacttcct
    10621 aagaaaggcc agcgcagcta ccagctcgcc gcggtcgatc tgaagaagat cgacgacatc
    10681 gattcgctgc gtgcggcgat cgacaagctt cgcacggaga tgcggcaagc ggctgctgat
    10741 ctcgagttcg agaaggcggc ggctcttcgc gacaaggcac gcgagctcga gcagctcgag
    10801 ctgcaaatgc gctgaactgc gcgacaccgc gtcgggcgtc gggtacacag gatcgacgat
    10861 gtttctctca gggttctacg cggtcctcga tcgagatgac gaagcgctcg cgcgcatgct
    10921 tgtcggtccc ggcggcgcga agatcctgca ggttcgcatc aagcccggcg aagcacgcga
    10981 cgtcgtgaag gtcgccaaga tggcgcgcaa agtctgcgac gaagccggcg ccctgctggt
    11041 catcaacgat cgcatcgata tcgcgctcgc cgttcgcgcc gacgctgtcc acctcggcca
    11101 gacggatctg tcgctaccgg acgcacggtc gatcgccgga gaccgcctcg cgatcggcgt
    11161 gtcgacgcac aacctgcacc aggtcgaagc cgcggtcgca cagcgtcccg attacatcgc
    11221 ctacggtccg atcttcgaga cgaccacgaa gcagaatccc gatcccgtgc agggcctcga
    11281 tgcgctgcgc gtcgcggtcg aagcggcggg cacgagcccg gtcgtcgcga ttggtggcat
    11341 cacgccggcg cacgcagctg atgtctatgc agccggcgcc gcggcaatct gcgcgatcag
    11401 cgccgtgaac aacgcggcag atgtcgtggc tgcagcgcgc gcgttcgcgc gtgtatgatc
    11461 gctccgcgtg tgtccggacg acgatgatct aggctcggcg aagaccgcga tcggcggcac
    11521 gatgccgtcg cagccgggcg tctcgcgtgt cgtcgcggaa ccggtgacga gcgtgatcgc
    11581 ggaccgctac gaggtccaag cgctgctcgg gatgggcggc atgggccgcg tgtaccgcgc
    11641 gctcgatcgc acgctcgacg atgtcgtcgc gctcaagatg ttgcgccgcg agctgctcga
    11701 cacgcctggc gtactcgaac gctttcgcca agaggtgaag ctcgccagac gcgtcaccca
    11761 cgcgaacgtc gtccgcacgt tcgatctcgg tcagcatggc caggatcact acctgacgat
    11821 ggagtacatc gaaggtcggt cgctcgcgca gctcatcgat gatggtcctc tagcgttcga
    11881 cgagatcatg cgcatcgcgc gcgcggtcgc cgccggcatt gcggctgctc atgcgaacgg
    11941 tgtgctgcat cgcgatctaa aaccggacaa cgtgctgatc gcgaagaccg gtcgcatcgc
    12001 gattaccgac ttcggcatcg ctaccgcgag cacctctccg acggtcacca gcgatcggct
    12061 cgtcggcacg cctgcgtaca tggcgccgga gcagctcgag gagggcgcga cgatcgctgc
    12121 cccggccgac gtctacgcct tcgggacgat cctgttcgag atggcgacag gtcgccgccc
    12181 gttcgtcggc aacgatccga tccaggtcgc gctcgctcgc ttgcacacgc cgcctccgga
    12241 tccccgcacg ctgcgccagg tccccgaagg gctcgccgag ctgtgtatcg cgtgcatggg
    12301 acgccgcccg gagacgcggc cggccgatgg cagtggtctc gtcgctgcgc tcgcatccct
    12361 cagcgatctc agctcgcgtc ctcgcaccgg ccggctcgcg atggatacgc ccacgcgaac
    12421 gtcgcgctcc gtcgccatcc tgccgctgcg tgggaccggc gatctcggcg acgttgccga
    12481 gggcctcagt gaagagatcg tcgacgcact ctcgatgacg cgatcattgc gcgttcggcc
    12541 gctcgcctct gttcgcaaag cggtgcgtgc cgattcagac gcgcgcgatg tcgggcagca
    12601 actcggcgtc gatgtcgtcg tcgagggttc cgtgcgccgg cgaggcgatc acgtgcgaat
    12661 caccgcgcgc atggtcagcg ttgccgacgg ctttcagctg tgggcgagtc acatcgacac
    12721 gggcaccgat gggctgctgg ccgcaagcga cgatctggtc gaagcgatcg caaaggccct
    12781 gacggttgat ctcgcgatgc cggaccgtgt gatggtcgat cccgaggcga gctcgctcta
    12841 tctcgagggc aaggcaaagg ttcgcctcaa ctggatcgac ggcaacatgg agccggccat
    12901 cgagtccttg cagcaggcgc tggagctcgc gccgaacgat ccggcgatcc tcgctacgct
    12961 ttcgcaggca ctcgcacgct cggcgttcta tggagctccg gcttctcgga ttgcacaggc
    13021 gcgcgagctt gccgaacgcg cggtcgagct tgcgccgcga atgggcgtgc cgcacatcgc
    13081 cgtcggcacc gcctctctct acgatggcga tgcgccagca agtgtggccg cattcgcgcg
    13141 cgcactacag cactcgccgg gttcggccat ggcgcaggcg atgctcggcg ccgccctgct
    13201 cgaagccggt gcgctcgacg acgcgattgc ccgcctcgaa gtctcgcggt cgctcgatcc
    13261 cctcggtctc gcgcatatcg atctgccacg cgcgtacttc tacgccggtc gttgggacga
    13321 cacggtcgcg gagtacgaac gcgcgcgcgt cgcgaaggac ttcgacctgc gcctcttcga
    13381 cgacatcatg ttcgcgcggc tcaagctgtg gcgcggcgag ctcgctccga ttgcggaggt
    13441 gcccggcacg tcgacgccgc cgaacgtctt cgcctatgca gaggttgttc gccgcatcca
    13501 cgcgacaggc gtcttctccg cggctgaccg cgccgcgatg gccgcgatcg tcgacatcga
    13561 caaccggcgg cttcgcgcga gtcgcgcgca gttcatggcc gagtacctga tgtttgcggg
    13621 cgagcccgac gaggcgctgc gctacgtccg cattggtgtc gaggcggggc tgcaagacta
    13681 cctctgggca cagaagtgcc cgctccttgc gcctctgcgt gcgcgggcgg aattccaagc
    13741 gctcgaggca acagtggccg agcgttcgcg agcggttatc gccgcatcgc gcgcggcgtg
    13801 attacttctt cgccggggcc gcggcgcctg tcacgagcgt gccgccagct ttcttgaact
    13861 gatcgacgcc gtcggccttg agctcgtact tcggcgccag cttcgagtgg cactgattgc
    13921 agctcttgat cttcgcgtcc ttcatgaact tcttcatcgc gtccttcgcg gcgggctggc
    13981 cacccttcgc gcaggcgtct ttcgcgagct gcgtcttgaa ctctttcgtc acgcacgccg
    14041 tgacggcctg ggcatcactg gcggttagcg acgcgattcc ccgcgaggcg acgatcgtgg
    14101 cgatggaagc aaaaatcgca agtttggcca tgggcgattg gtccttggac ggccccgtag
    14161 gtcagctatt cagggcccgt actttgtagc aataaatggc gcggatccgg gcgtttgccc
    14221 gcagtgaaac ggaccgtccc gatcctgctt cttctgtgcg gctccgcgag tgccgacacc
    14281 ctcacgctcg cgcctgtgcc ttctgccagc gaaccgaacg tcgtcggcag caaggccgtc
    14341 tgcaaaggcg acaacgactt ccttcgcgtc gcgcgcacga agctcgacgg ccacgaggtc
    14401 ctcgtactcg gtgactgtgc gggaacgcac tcgtatctgg cgctcgagac caagtcgggg
    14461 tggacgcggt tcgtgagcgc gcctggctgg ggttcggtga cgagcacaaa gctcaagaag
    14521 cgcgacgtgc tgctccatcg gatcgatacg tacgtcgacc aagacaagtc gacgagccgc
    14581 gtggatctct gcgtctacga cgacaaggcc ggaacgccat cgtgcggctc tgccgaggtc
    14641 gagtgtccgg agaccggatg ccgcgagcca gagatcatca agggcgcgct gtggatgcac
    14701 gctgcgggtg gtcgcaagcg ctttctgatc gactagccgc gcgcgatcac gaagagcact
    14761 tcgctttgtt cttcgaatcc ttcttgagcg tcgcgtcgag atcgcccgcc tgtttcagca
    14821 cgttcgactt cggatacttc gacttcacga gccccgcgta cgtgcgcgct tcggtgcagt
    14881 tcttcagcga ctgcgcggcg agcgcggcga agtagagacc gtcgtcggcg agatcgctgt
    14941 ccggatactt ctggacgagc tgctggtacg cggcgatcgc gttgtcgaag tctttgaggt
    15001 tcgtatacgc ctggccgcgg aagtactgcg cgtcatcggc gcgtgcgtgc gtcgggaatg
    15061 tctggacgag gcgcttgtag atatcgagcg cgtcgttgta gcgagccgcc tcgaatgccg
    15121 tcgtaccgag cttccacagc tcgtcggggc cgctgttcgc cgtcggcttg ccgctctcga
    15181 tttgcgcgac gcgctgctcg agcgagtcca tgcgcgcgtt gttctcggtg cggtacttgt
    15241 cgaagccgac cttcagctcg ttgatgctgt tgttgatcgc agcggtgagc ccgttggccg
    15301 tgcggatgtc tgccgcgagc ttctcgacgt cggcaccgat gtcggcgctg ttgcgcttga
    15361 ggagcttcgt cgcttcgtcg agcaccgctt gcagctgctt gatctgcgtg tcgagctctt
    15421 tctctttcgt gtcgacgcgg ccttggagcg cggtgatgtc gcgccgcatg gcctgaccct
    15481 cactcttcgt cgtcgtccag aagcagccgc ccaggaggaa ggacgccgcg acggcggcga
    15541 gagtggcagt agctttcaac gccatttgaa ctccacgcgg cgatccttgt cgtcgccgtt
    15601 gccagtgggc tcggtctcgc ccttgggaac cacttgtagc tttgccgggt cagagccgag
    15661 tcgcgcgagg tagtcggcca cctgctgcgc gcggcgctcg gacagcgcga tgttgtactc
    15721 ctcggtgccc gacgtatcgg tgtgaccgat cacgagcacg gccttgtcct tgttcgtgga
    15781 catgcacgcg ttgttcttgt cgagccggtc gcgctcgctc gcctgaatcg tcgagtcgtc
    15841 gaacgcgaag tagacggtgt tgagcgtgca gcccgcatcg acattgctgc cggcaccggc
    15901 gcgcacgcag tagccgttga tgcagtcttc gtcgtcggcg cactcttcgt ccttcgtgca
    15961 cttcttgccg cgctgacacg cgcctgcttc gcacttgccg ccggggccgc actcgccgtc
    16021 gcccgcgcat ggcttgcacg agccggcttg gcacacctgg ccggacggac attgatcgtc
    16081 ggtgatgcat gcgggcttca cgacgcacgc gttgccgctg cacttcttgc ccttcgggca
    16141 ctgcgaatca tcgcggcact cgacgcattt gttggtcgcg cagacgagcg gcttcttgca
    16201 gtcgttgtcg cccttgcagc ccggtggctt cggtttgctg ccgcatcccg ccgccagcgc
    16261 tagcgtgatt acggcgatcg ctacgaagac cttgcccata ggccctgaaa cctaaatgga
    16321 cgcagcacgt tagtcaaatc tggtagggtc tctcgcgtgg cgcgcagcac gattctcgtc
    16381 atcgatgacg agccgaacat cccgaccacc gtgcggcgct cgctcgagct ggagggctac
    16441 tccgtcgagg ttgcgggtgg tggcgccgct ggattggcca agctggccga gcacgatatc
    16501 gacatggtcc tgctcgacgt catgatgccc ggcgagaccg gtctcgaggt gcttccgaag
    16561 atccggacca tgaagcccga gacgatggtc gtcatgatgt cgggcaacgc gacgatcgag
    16621 acggcagtcc acgccaccaa ggcgggcgcg tacgacttca tcgaaaagcc gctgtccggc
    16681 gacaagctgc tgatcacggt ccagaacacg ctcgcgttca agcgcctgcg ctacgagaac
    16741 cagcgcctgc gcggccgcgt gcagaccgac ttcgcgatga tcggcaaggg cgccgcgatg
    16801 aaggcga
//
LOCUS       DATLBZ010000420        17137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6966801, whole genome shotgun sequence.
ACCESSION   DATLBZ010000420 DATLBZ010000000
VERSION     DATLBZ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17137
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6966801"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VMZ53_21365"
     CDS             complement(<1..1202)
                     /locus_tag="VMZ53_21365"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HUS31080.1"
                     /translation="MEPFEPHLALLTALAVGVLIGLEREQAKEKSPGSQFAGIRTYPI
                     FALTGALAMLLESASIWLPLIALAGVITLVAIHYAHDVRRDAAHGVTTEASVIVTYLL
                     GALAASRGVIEPMATRLLLVGGLGVTLTFLLSSKDWLHGLVGRVSREDFFSTVKFLIA
                     AVIILPLLPREELGPLDAINPFTVGLMVVMISGLSFAGYVAMRLLGKGRGLLVSAAMG
                     GLVSSTAVTIAFSGRTKHDPSMAPAAAGAIAIASTIMLARVGVLVALINPGLLSLLII
                     PLGAAAAGAVIGGVLMYRRAEETKVDELPVKNPFELGNAIRFGLVFALILLVTKAAKQ
                     YLGDAGLYLAAALAGTTDVDAVTLSTAKHAETGQTAAVIAIMIATVSNTLVKSSLALG
                     IGGAKLGKR"
     gene            1232..2677
                     /locus_tag="VMZ53_21370"
     CDS             1232..2677
                     /locus_tag="VMZ53_21370"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HUS31081.1"
                     /translation="MWQPPARIKHELPAPSWPTPEQAAGARLYQPQAIGPRKALQRTW
                     VPAMVPWRATEDGFVTRDVIDWYSRFAQGRPGVIVVEATGIRDIPSGPLLRIGHDRFI
                     PGLRDLVHAVGEASHGETLLFIQLIDFLSIRRRPPRDKFFGRFLAITEEHRAAVGGGT
                     DDEVRAKLAELPEAELVKVLSQRELEDLERGYRERVTDTHLPHIEELPRVLPGLFSDA
                     AGRAMEAGFDGIELHFAHAYTMSSFLSALNTRADGYGGSRENRVRLPREVVTAVRTKV
                     AGRAVVGCRYLGDDVVEGGNRVDDAAWFGVELARAGLDFLSISKGGRFEDAKQPKVGW
                     SAYPYTGQSGYECMPTIYSDEKGPFSRNVSLAATIRTAVRDAGMNTPIVTAGGVCEFA
                     QAEAILEHGEADFVASARQTLADPDWFLKIREGRGSEIRRCEFTNYCEALDQQHKQVT
                     CKLWDREELDAPDVTLASDGKRRLVAPRWRR"
     gene            2763..3116
                     /locus_tag="VMZ53_21375"
     CDS             2763..3116
                     /locus_tag="VMZ53_21375"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HUS31082.1"
                     /translation="MKFRRTLGATTLIHDKVVNLSGQYVGRIEELMVDVTTGRVAYAV
                     LSHGGILGFGGKLFALPWSALTVDESKKRFVVNVSAEALDRMPGFDKDHWPDLNDLEY
                     AKGVYRQWGATPHWT"
     gene            complement(3161..3418)
                     /locus_tag="VMZ53_21380"
     CDS             complement(3161..3418)
                     /locus_tag="VMZ53_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31083.1"
                     /translation="MKPCETCGNTYDKAFEVSIGGTTHTFDSFACAIHMLAPRCNHCK
                     VTIIGHGMEARGQYFCCASCARQAGVQELSDRADDRGTASP"
     gene            3524..4597
                     /locus_tag="VMZ53_21385"
     CDS             3524..4597
                     /locus_tag="VMZ53_21385"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUS31084.1"
                     /translation="MVGLGHFAQAAVLPAIEQLDDVELTALFSGDDEKLHELGERYGV
                     RTLAHYDELDSLLAKGLVDAVYIAVPNDLHAEMTLVAARHGVHVLCEKPMAPTEPECM
                     QMIRACEQRRIKLMIAYRLHFQAANLIAIELIRGGELGDPRVFNSTFSLQVRDGNTRT
                     QPRRGAGPLFDIGIYCLNAARYLFRSEPLEVSAIKTSGRDPRFASIDEAYAVTMRFPF
                     ERVAQFTCSFGAHDRAHYEVIGTEGLLTLDNAYEYAAEMYMTVDGKHGKKQRTFEKRD
                     QIAGEIEYFARCIRDDVEPEPSGWEGLADIRILQAIQQSARFGRCVPIEPILRPTRPD
                     MRQEIDIEPHPEPRLVKVQSSSK"
     gene            4631..6454
                     /locus_tag="VMZ53_21390"
     CDS             4631..6454
                     /locus_tag="VMZ53_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HUS31085.1"
                     /translation="MRWVVALCLVAGCGRLGFDAVTGDDVPATCDAAPEPNDSIATAT
                     PLELGRETGGRLCKGDVDVFAIELAQAEEVAVTITSEGPGWPSIELVSNDGARAPKTY
                     ASRLTGFADRTGTFYVRVAGVAPVDGTPYRLTVTKLTGRHIFIAPTGNDAGPGTFDVP
                     LQTFDAAFGRAMPGDTLVLRDGDYTVANNNRIWATCNVDAMSGTQDAPIRLVAFTERR
                     AFMLGDGNEDAIRFDGGCAYWSIEGLRVENQDRVGGTSGGAITFNNTSHMTIRRVLAR
                     KNNRFINSHVITIADSSDILVEDSEIYDFHRNGLLFYNTQRATARRIYIGSRGSTDVS
                     GGYVSDPADKGDTGVAVSHSRDALVENVISEGNGAQSNVSGYDIDTTDARWLGDVALD
                     NDYGFISSILTGGAQNTVRTRYENCVSVGSASVGFYIRSNMDTSCTACTAIGSATHGW
                     DADLLSAQPRTASSHCINCLAIGSGMNGFYVQQQTGGWSLESANAFNNGAPFDPPTAP
                     ELTAPMQLDPKLGGCLVYEPVNAPLRGAGVGGGTIGAEVIYRYVDGIRTTAPLWDPET
                     GAFPCGAIVEGVNDSAAGSCIGVHNRLHVGAPDCPLPYPAL"
     gene            6462..7214
                     /locus_tag="VMZ53_21395"
     CDS             6462..7214
                     /locus_tag="VMZ53_21395"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUS31086.1"
                     /translation="MDLLHVALIALAACAAGAVNAIAGGGSLITFPVLLAFGVPPVIA
                     SMTNTVAMCPGYVGATIAQRRELVGQGGRLARILPFAIVGSVVGSVILLHTTNRAFET
                     LVPFLLTFAALLLAVQGRLRRYLLERTHVAHSILLAGIPLGLAAAYGAYFGAGMGMII
                     LAVLAVVIDDTLVRANALKQVISLVVNVLAALIYIVIGTIHWHVAAAIAVGSLVGGAL
                     GGMLVSKIKEVWLRGVAITVALVVAAIYFAKL"
     gene            complement(7450..7776)
                     /locus_tag="VMZ53_21400"
     CDS             complement(7450..7776)
                     /locus_tag="VMZ53_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31087.1"
                     /translation="MQMDVLEHGSSPLKNERKKCGRRRWEPTRHGQIDRDRVAVARGL
                     RRRDANGDGMSRDAARGRETGVQVTTLRIDASIAMAWPCKHDRATTDDDLLRFFTVYE
                     TVGMLG"
     gene            7753..9222
                     /locus_tag="VMZ53_21405"
     CDS             7753..9222
                     /locus_tag="VMZ53_21405"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HUS31088.1"
                     /translation="MFQYIHLHLNERAVLVRNGKAVRALTPGRYTFFKRYEVYRWNID
                     QVVFHTLPVVLEALPPEWYETVRLAQGQYGIVYVDERPTQFLRPGVHRVWTLNGDVRL
                     RAFVETDPLPELTTELRDVIPKDELLETTIDLNQRAVLLRDGVPERVLVPGHYAFWGK
                     HNKLLTWKIDDLAFWAQPEVLAMLPQTWFQTIDLADTQRAVVYRDDKPMKFLRPGIHR
                     VWMLDPNVAIRVFDVLGPAPEMTDELRVIIPAAEVVEAQVRQFEKGLLYVQGRFAGIL
                     EPGRHAFWNHPGARITVNVLDTRVQQLKIEGQDLMTRDKVTLRLTLTAEYAPADAPTT
                     VHAVADVKEALYLAVQLAAREYVAGVTLDELLEGRDALTRYLEAQVQPRAEMFGVRLH
                     RVGVKDVILPGEMKTLLNKVIEAEKAAAANVILRREDAAATRNMAQTAKVIAENPVLM
                     RLKELETMKDIAEKIDEIKLVMGMDGLANILPLANGKHD"
     gene            complement(9374..10225)
                     /locus_tag="VMZ53_21410"
     CDS             complement(9374..10225)
                     /locus_tag="VMZ53_21410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31089.1"
                     /translation="MGVRSLVVLLVAVAACGKAADKPATTGSAGSAGSAAVPESKNVL
                     VFVDDAQVAQVTPEQVAGWPRVDTLVPTDARRLGKWKTVTLEASSPKPIDINNPSSTF
                     PELVPALFPGADGAPAFGYFDPVELAKKGTPQMRQDHLHDVHIKLAQNSGRGENEQGD
                     GGGMDPSKLELVVKASGKETKLMGTQLLEMPRQPPPGENGGMGWPLAAILEKAGVTKY
                     EKLIATDSKGTNLVLEKQDLDPKAAMPFVKLNRQGTLRFIVFKKNGETWGRGGDLREL
                     SRVEVVK"
     gene            complement(10245..11927)
                     /locus_tag="VMZ53_21415"
     CDS             complement(10245..11927)
                     /locus_tag="VMZ53_21415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS31090.1"
                     /translation="MSKPRVAGRTLALVVVLELVAGACIWFSHSPSADAQPSQSASAA
                     GSAAVTQPAADAPKVVETKDACPVPPKKPHVKKTFTQVAKDDLLPIGILLAVIVLVLW
                     RLPKVELGHTPAFRSRRLLNWLPLGLTYSFLYFARYNIKSFQGVGLTEAEYGTVFGIG
                     SAVYGLAFLINGPLTDRWGGRATILISAAGAGVANLWMGYMAATKNTFGMSVPTAFTA
                     AFAMNMYFQSFGAVSIVKVNAAWFHLRERGTFGGIFGILISLGLYFAFDVIGRIKENY
                     SIQWIFLVPAMILFVFFVLSFVFVRNEPADAGHPNFDLGDATNKADEDTPKLTFWVWL
                     GQIRFVLWKLISHPVLLTIALIELCSGFLRQAILQWGPDFGKGVGVSSSFVFKNWGMV
                     SCIAGITGGMFAGALSDHLFQSRRSPVSAVLYGIMIGGSIMILVLLDMPMSIGWVVAF
                     MSMAIIGVHGMLTATASADFAGKKNTGVAVGLIDGFVYLGSTVQSFVYGHALPASKMR
                     DAANCLVPNPEYAKSANWSVWPIAMIPIAIVGFLLTLRIWNAKPRSGSTGGH"
     gene            12213..14285
                     /locus_tag="VMZ53_21420"
     CDS             12213..14285
                     /locus_tag="VMZ53_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31091.1"
                     /translation="MTQRITLSIALAASLLTAACAIDDKVDPGAGGEGEGSDYSGSGS
                     EDVPMDPGYGGGMDMTDPDVNVQPTYPTAHPRIYVNANRARLQAALAANTTPASRFRT
                     TVDNQINGANYWEFRAWNIALMGQLSGDPKYCTKAIAQVDAFVASEEPKIAAGTQPTV
                     AGNSYLYVGDYLGDLALTYDWCFDGLTASQKTRWQTYADTTLWNLWHPTQAKWGGKTL
                     AWTGWSTNNPGDNYYYSFMRATMLVGLAMKGETAQGDTWVNTFRDEKILGQLVPMFDK
                     DLLGGGSREGTAYGVSMRGLFHLYDMWHDTTGESLKSKTTHARRSMRLFVHQTMPTLD
                     RFVDSGDQPRDAAAMFFDYQRNYLQELVQMYPNDSVAPKAKALLEGSTVTQLARPETL
                     VFDFLYDMNNVTSQPLGGMGTSLYAPGIGQVYSRSGWDKAATWVNFTAGAYAESHQHQ
                     DQGQLVIFKEGWMMADAGLGSRNGIIQDTPAHSIVRIDSGSTPVKQMVNTESKLVGLH
                     GADTWLYAAADLTPAYGGNAAVQKVQREFVFLKPNVIVVYDRVATASGTSQTWQLASP
                     TAPSISGATTTVAGAHPMTVRRLVPASATAAVTNMTSVTSTAGSYLSGYRLDEKVAGG
                     DQRYLHVISLDGAATNATVSGDSGVTVSLSDGSTATVSFNRDTIGATLAWGSVSETLG
                     AGVDMLAQ"
     gene            14345..15151
                     /locus_tag="VMZ53_21425"
     CDS             14345..15151
                     /locus_tag="VMZ53_21425"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HUS31092.1"
                     /translation="MPLWMRTTLRLSVVVLVAAAASIFGLVLVRQVIDYDSLRASSDS
                     LGTFVQTLGGIYAVLLAFVVIVVWGQFNDARGYVEREANALVDLHRTASGLPVHSRTI
                     IQKELREYVDAVITDEWQAMSKGDVHTIERITGRLDHVWTAIHTCEPRNECQQTMHSE
                     VLSRFNDLTEHRTNRLNSAGARIPIALNVLLYTGAVIMIGAVYLLAIDRFWLHALVTG
                     LMSGAVAHILFLIYDLDHEFAGDWQVEKTAFVRARRAFERTEELHTHEPL"
     gene            complement(15155..>17137)
                     /locus_tag="VMZ53_21430"
     CDS             complement(15155..>17137)
                     /locus_tag="VMZ53_21430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31093.1"
                     /translation="YAHSESVLHRDLKPSNVLIGPFGETVVIDWGLAKEIRDEDGQLA
                     SDSGARRKQRRPATPLPDERVEADERASDANLTQVGAVLGTPSYMAPEQARGEPSDER
                     SDIYALGALLYNILTGEAPHRGKTTDEVLAQVAAGKHVPIAEREAELPHELAELVEHA
                     MAHEPSKRIASAKELAEELRRFAAGKLLASHAYSLGELVKRWIWRHRVTVGISAAAVV
                     LLASIGVYAFLSVMRAKNVAQEQTKQAVAAKNEAMQANLNLRDANNRDKLKMAQQSLD
                     IDPSRAVAWLGLMGDEGFRWESTHAIAVEAARDGLAQELRGHTEDVELVVVSPDHKHV
                     ATGSDDSTIRWWNLDDRTSVELRGHNGPIETLAMSKDGKWLASAGTDHDVWLWELATG
                     TGRKLSGHSNTVRGVAFSPDDTKLASTSEDGSLYVWDVTSATGKQLLRHNHGLRPVIW
                     YDDHTVFVGGYDGNMGRVDVVTGKGAMKPAHAAELRCFALSPDKKWLIAGDEDGLVTL
                     WTADGARVKTLGRHTDVTRKVIFTPDGSHAVSAGGDSRVILHAIPEGTELELEGNNAG
                     VKDIAMSADGSLVASAGIDGVVRVWTLPDGKLRNEFHGHVFAVKNVAFTNDGRLVSGS
                     EDNRARVWTLDESDDPPKGLPLRAWLTAHSNVQVAD"
BASE COUNT         3053 a         5533 c         5527 g         3024 t
ORIGIN      
        1 cgcttgccga gcttcgcgcc accgatgccg agcgcgagtg acgacttgac gagcgtgttc
       61 gacaccgtcg cgatcatgat cgcgatgacg gcggcggtct gccccgtctc ggcgtgcttt
      121 gccgtcgaga gcgtgaccgc gtcgacgtcg gtcgtgccgg cgagcgcagc ggcgaggtag
      181 aggcccgcgt cgccgaggta ctgcttcgcc gccttcgtca cgagcaggat cagcgcgaac
      241 acgaggccga agcggatcgc gttgccgagc tcgaacgggt tcttgacggg aagctcgtcg
      301 acttttgtct cctcggcgcg ccgatacatg agcacgccgc cgatcacggc acccgcggcc
      361 gcggcaccga gtgggatgat cagcagtgag agcaggcccg ggttgatcag cgcgacgagg
      421 acgccaacgc gcgcgagcat gatcgtcgac gcgatcgcga ttgcaccggc ggcggcggga
      481 gccatcgatg ggtcatgctt cgtgcggccg gagaacgcga tcgtcacggc ggtcgatgac
      541 acgagcccgc ccattgctgc actgacaagc agcccgcggc ccttgccgag caggcgcatg
      601 gcgacgtagc ccgcaaacga gaggcccgag atcatgacga ccatcaggcc gacggtgaac
      661 gggttgatcg cgtcgagcgg accgagctcc tcgcgcggca acagcggcag gatgatcacc
      721 gcggcgatca ggaacttcac cgtcgagaaa aagtcctcgc gcgacacgcg accgacgagg
      781 ccgtgcagcc aatctttcga cgacagcaag aatgtcaacg tgacgccaag cccgccgacg
      841 aggagcaagc gagtcgccat cggctcgatg acgccgcggc tcgctgcgag tgcgccaagc
      901 agatacgtga cgatgacaga cgcctcggtc gtgacgccgt gggccgcgtc gcggcggacg
      961 tcatgcgcgt agtgaatcgc gacgagtgtg atgacgccgg cgagcgcgat cagtggcagc
     1021 cagatcgacg cggactccag cagcatcgcg agcgcgcccg tgagtgcaaa aatcgggtag
     1081 gtccggatgc ccgcaaactg cgagcccggc gacttttcct tggcctgctc gcgctcgagg
     1141 ccaatgagaa ctccgaccgc caaagctgtc agcagcgcga ggtgtggctc gaagggctcc
     1201 accgtctcca gtgtggtacg aatcggcggc catgtggcag ccgcctgcgc gtattaagca
     1261 cgagcttccc gcgccgtcgt ggccgacgcc cgagcaggca gcgggcgcgc gtctgtacca
     1321 gccgcaggcg atcgggccgc gcaaggcgct gcagcggaca tgggtgcctg cgatggtgcc
     1381 gtggcgagcg accgaggatg gcttcgtcac gcgcgacgtc atcgactggt actcgcggtt
     1441 cgcgcagggc cggcccggcg tgatcgtcgt cgaggcgacc ggaattcgcg atatcccatc
     1501 ggggccgctg ctgcgaatcg gtcacgatcg cttcattccc ggtctgcgcg atctcgtgca
     1561 cgcggtcggc gaggcgtcgc atggcgagac gctgctgttc atccagctga tcgatttcct
     1621 ctcgattcgt cgccggccgc cgcgggacaa gttcttcggc cgcttcctcg cgatcaccga
     1681 ggaacatcgc gctgccgtcg gtggcggcac cgatgacgaa gtgcgcgcga agctcgctga
     1741 gctccccgag gcggagcttg tgaaggtgct ctcgcagcgc gagctcgaag acctcgagcg
     1801 cggttatcgg gagcgcgtga ccgacacgca tctgccgcat atcgaggagc tgccgcgcgt
     1861 gctcccgggc ttgttctccg atgcagcggg tcgcgcgatg gaagcgggct tcgacggcat
     1921 cgagctgcat ttcgcgcacg cgtacacgat gtcgtcgttc ctctcggcgc tgaacacgcg
     1981 cgccgacggc tacggtggct cgcgcgagaa tcgcgtgcgg ctgccgcgcg aagtcgtgac
     2041 ggccgtacgt acaaaagtcg caggccgcgc agtggtcggc tgtcgctatc tcggtgatga
     2101 cgtcgtcgaa ggcggcaatc gcgtcgacga cgcggcgtgg ttcggcgtcg agctcgcgcg
     2161 tgcgggtctc gacttcctgt cgatcagcaa gggcggccgc ttcgaagatg ccaagcaacc
     2221 aaaggtcggc tggtcggcct atccgtacac cggacaatcc ggctacgagt gcatgccaac
     2281 gatttactcc gacgagaaag ggcccttctc gcgcaacgtc tcgctcgctg caacgattcg
     2341 cacagccgtc cgcgacgcgg gcatgaacac cccgatcgtc accgccggtg gcgtttgcga
     2401 gttcgcgcaa gccgaggcga tcctcgagca tggcgaagcg gacttcgtcg cgtcagcgcg
     2461 ccagacgctc gcggatcctg attggttcct caagatccgc gaaggtcgtg gcagtgaaat
     2521 ccgccgctgc gaattcacga actactgcga ggcgctcgac cagcagcaca agcaggtgac
     2581 ctgcaagctc tgggatcgag aggagctcga cgcgcccgat gtgacgctcg ccagtgatgg
     2641 taagcgccgg ctcgtcgcgc cgcgctggcg acgctagcta gagatcctcc taggaggaca
     2701 gcgatccgag cggtgagcct ctgtggtttt ccgatggaac accaattgga ttgctcttca
     2761 gcatgaagtt tcgacggacg cttggagcaa cgactctcat ccacgacaag gtcgtcaatc
     2821 tcagcgggca atacgtcggc aggatcgaag agctgatggt cgacgtgacc acaggtcgag
     2881 ttgcgtacgc agtgctctct cacggcggca ttctcggttt tggcggcaag ctcttcgcgc
     2941 tgccgtggtc tgcactaacg gtcgacgaat cgaagaagcg gttcgtcgtg aacgtcagcg
     3001 cagaagccct cgatcggatg ccgggattcg acaaagacca ctggccggat ctgaacgatc
     3061 tcgagtacgc gaagggcgtg tacaggcagt ggggcgccac tccgcactgg acgtgagcgc
     3121 gggtgcacga aacggggcac gtgcgcgggc gcgcgtcgtg tcagggtgac gcggtgccgc
     3181 gatcgtcggc gcgatcgctc agctcctgca cgcctgcctg acgagcgcag gatgcacagc
     3241 agaagtactg accgcgcgcc tccatgccgt gcccgatgat cgtcaccttg cagtggttac
     3301 agcggggcgc gagcatatgg atggcgcacg cgaacgaatc gaacgtgtgc gtcgtgccgc
     3361 cgatcgacac ctcgaaagct ttgtcgtagg tattgccgca cgtctcgcag ggcttcatgg
     3421 cgccgcagtt gtgcaatcaa cgagccgttc tgaggcattt cgtggaatgg ggcttgctgt
     3481 atctagaggt atgagtcgcg cgacgaaacg tctgagaatg gccgtggttg gactgggaca
     3541 cttcgctcag gccgcagtac ttcccgcgat cgagcaactc gacgacgtcg agctcactgc
     3601 gctgttctct ggcgacgacg aaaagctgca cgagctcggc gaacgctacg gagttcgcac
     3661 gctcgcgcac tacgacgagc tcgattcgtt gctcgcgaag ggtctcgtcg acgcggtgta
     3721 catcgcagtt ccaaacgatc tgcacgccga gatgaccctc gtcgctgcgc ggcatggcgt
     3781 tcatgtgttg tgcgagaagc cgatggcgcc gacggagccc gagtgcatgc agatgatccg
     3841 cgcatgcgag cagcggcgca tcaagctcat gatcgcgtat cgcctccact tccaggccgc
     3901 gaacctcatc gcgatcgagc tcatccgcgg tggcgagctc ggcgatccgc gcgtcttcaa
     3961 ctcgacgttc tcgctgcagg tgcgcgacgg caacacgcgt acgcaaccgc gtcgaggcgc
     4021 cggtccgctg ttcgatatcg gcatctactg cctcaacgct gcgcgctact tgttccgcag
     4081 cgagccgctc gaggtcagcg cgatcaagac gagtggccgc gatccgcgct tcgcttccat
     4141 cgacgaggcg tacgcggtga cgatgcggtt cccgttcgag cgcgtcgcgc agttcacgtg
     4201 cagcttcggc gcacacgacc gcgcgcacta cgaagtgatc ggcaccgaag gcctgctcac
     4261 gctcgacaac gcgtacgagt acgcggccga gatgtacatg acggtcgacg gcaagcacgg
     4321 caagaagcag cgcacgttcg agaagcgcga tcagatcgct ggcgagatcg aatacttcgc
     4381 gcgctgcatc cgcgacgacg tggagcccga gccgagtggc tgggaaggcc tcgccgacat
     4441 tcgcatcctg caggcgatcc agcagtccgc gcggtttggc aggtgcgtac ccatcgagcc
     4501 gatcctgcgc ccgacgcggc cggacatgcg acaggagatc gacatcgagc cgcatcccga
     4561 gccgcggctc gtgaaggtcc agagctcctc gaagtaacgc tgcggccgca aatgacctta
     4621 tgatccagcg atgcgctggg tggtcgcgct ttgtctcgtc gcaggttgcg gccggctcgg
     4681 cttcgacgcg gtcacgggcg acgacgtgcc ggctacctgc gacgccgcgc ccgagccaaa
     4741 cgattcgatc gcgacggcta cgccgctaga gctcggccgc gagacaggtg gccgcctctg
     4801 caaaggcgac gtcgacgtgt tcgccatcga gctcgcgcaa gcagaagaag ttgcggtgac
     4861 gatcacctcg gaaggtccgg gctggccgtc catcgagctg gtcagcaacg acggtgctcg
     4921 tgctcccaag acgtacgcat cccggctcac cgggttcgcg gatcgcaccg gcacgttcta
     4981 cgtgcgtgtt gccggtgtcg cgccggtcga cggcacgccc taccgcctga cggtcacgaa
     5041 gctgacgggg cgccacatct tcattgcgcc gacgggcaac gacgcggggc ccgggacgtt
     5101 cgacgttcct ctgcagacgt tcgacgcggc gttcgggcgc gccatgccgg gtgacacgct
     5161 cgtgctgcgc gacggtgact acacggtggc gaacaacaat cgcatctggg cgacgtgcaa
     5221 cgtcgatgcg atgagcggta cgcaagacgc gccgattcgc ctcgtcgcgt tcacggagcg
     5281 tcgtgcgttc atgctcggtg acggcaacga agatgcgatc cgattcgacg gtggctgcgc
     5341 gtattggtcg atcgaagggc tgcgagtcga gaaccaggac cgagtcggcg gcaccagcgg
     5401 tggcgcgatc acgttcaaca acacgtcgca catgaccatc cgccgcgtgc tcgcgcgcaa
     5461 gaacaaccgc ttcatcaact cgcacgtcat cacgattgcc gattcgtcgg acatcctcgt
     5521 cgaggacagc gagatctacg attttcatcg caacggtctg ctgttctaca atacgcagcg
     5581 cgcgaccgca cgccgcatct acatcggttc acgaggtagc actgacgtct cgggtgggta
     5641 cgtgagcgat ccagcggaca aaggcgatac gggcgtcgcg gtcagtcact cgcgcgatgc
     5701 cctcgtcgag aacgttatct ccgagggcaa cggcgcccaa tccaatgtct ccggttacga
     5761 catcgacacg acggacgcgc gctggctcgg cgacgtcgcg ctcgacaacg attacggctt
     5821 catcagctcg atcctcacgg gtggcgcgca gaatacggtg cgtacgcgct acgagaactg
     5881 cgtgtccgtc gggtcggcat cggtcgggtt ctacattcga tcgaacatgg atacgtcatg
     5941 cacggcttgc acggcgatcg ggtccgcgac gcacggctgg gatgcggatc tcctatcggc
     6001 acaacctcgg actgcgtcgt cgcattgcat caactgtctc gcgatcggca gcgggatgaa
     6061 tggcttctac gtccagcagc agacaggcgg atggagcctc gagagtgcga acgcgttcaa
     6121 caacggcgcg ccgttcgatc cgccgaccgc accggagctg acagcgccga tgcagctcga
     6181 tccgaagctc ggcggttgtc tcgtgtacga gccggtgaac gcaccgctgc gaggagccgg
     6241 cgtcggtggc ggcacgatcg gcgcggaggt gatctatcgc tatgtcgacg gcattcgcac
     6301 gacggcgcct ctctgggatc cggaaacggg tgcgttcccc tgcggcgcga tcgtagaagg
     6361 agtgaacgac tccgcagcgg gcagctgcat cggtgtccac aaccggctgc acgtcggcgc
     6421 gcccgactgc ccgctgccgt acccagcgct atagtgagga cgtggatctc ctgcacgtcg
     6481 cgctgatcgc gcttgccgca tgcgcggccg gtgcggtgaa tgcgatcgca ggtggcggca
     6541 gcttgatcac gtttcccgtg ttgctcgcct tcggtgtgcc gccggtgatc gcgagcatga
     6601 cgaacaccgt cgcgatgtgc ccgggctacg tcggcgccac catcgcgcag cggcgcgagc
     6661 tcgtggggca gggcggtcgc ctcgcgcgca tcttgccctt cgcgatcgtc ggcagcgtcg
     6721 tcggctcggt gatcctgctg cacacgacga atcgcgcatt cgagacgctc gtgcccttct
     6781 tgctcacgtt cgcggcgctg ctgctcgcgg tgcagggccg gctgcgccgc tatctgctcg
     6841 agcgaacgca cgtcgcgcat tcgatcttgc tcgccggcat tccgctcggt ctcgctgcgg
     6901 cgtacggcgc gtacttcggc gctgggatgg gaatgatcat cctcgcggtg ctcgccgttg
     6961 tcatcgacga cacgctcgtg cgcgcgaacg cgctgaagca agtcatctct ctcgtggtca
     7021 acgtgctcgc tgcgctgatc tacatcgtca tcggcacgat ccattggcac gtcgcggcgg
     7081 ccatcgcggt cggctcgctc gtcggtggcg cgctcggcgg catgctcgtg tcgaagatca
     7141 aagaggtgtg gctgcgcggc gtggcgatca cggtcgcgct cgtggtcgcg gccatctact
     7201 tcgcgaagct gtaagcgcac ttcgaaaaat gtcgttgacg atctttcgcg catgccgtag
     7261 ttctttgcgc ctcgctggcg tctaggggtt ggctcgagac caaactggcg acgttgcgca
     7321 tggcacaccg ccttgttcgc gttcgtcgtt ccgggaccgc gaaccgccaa agagaccagg
     7381 gtaagaaggt tcgactcctt ccgagcggct tcggccgcac gatgtggaac ggtcccacat
     7441 tgggcttgct catcccaaca tcccaaccgt ctcgtaaacg gtaaaaaacc tgagcaggtc
     7501 gtcgtcggtg gtcgcccggt cgtgcttgca cggccacgcc atcgcgatgc tcgcgtcgat
     7561 ccgcagagtc gtcacctgca cgccagtttc gcgcccgcgt gctgcgtcgc gactcatgcc
     7621 atcgccgttc gcgtcacgac ggcggagtcc gcgcgcaacc gccactcgat cacgatcgat
     7681 ctggccatgg cgcgttggtt cccaccgacg ccggccgcat ttttttcttt cgttcttcaa
     7741 aggagaagag ccgtgttcca gtacatccat ctgcatctca acgagcgcgc ggtactcgtg
     7801 cgcaacggca aggcagtgcg tgcgctcacg ccaggccgct acacgttttt caagcgctac
     7861 gaggtctacc gctggaacat cgatcaggtc gtgttccaca cgctgccggt cgtgctcgag
     7921 gcacttccgc cggagtggta cgagacggtt cgtctggccc agggccagta cggcatcgtc
     7981 tacgtcgacg agcgtccgac gcagttcctg cgtccgggtg tccatcgcgt gtggacgctg
     8041 aacggcgacg tgcgcctgcg tgcgttcgtc gagacggatc cactgccgga gctgacgacc
     8101 gagctgcgcg acgtgatccc gaaggacgag ctcctcgaga cgacgatcga tctcaaccag
     8161 cgtgcagtgc tcctgcgcga tggtgtgccc gagcgcgtgc tcgtgccggg tcactacgcc
     8221 ttctggggca agcacaacaa gctgctgacc tggaagatcg atgacctcgc attctgggca
     8281 cagccggagg tcctcgcgat gctgccgcag acgtggttcc agacgatcga tctggcggac
     8341 acgcagcgtg cagtcgtcta ccgcgatgac aagccgatga agttcctgcg tccgggtatc
     8401 caccgcgtgt ggatgctcga cccgaacgtc gcgatccgcg tcttcgacgt gctgggtccg
     8461 gcgcccgaga tgacggacga gcttcgcgtg atcatcccgg ctgccgaggt ggtcgaggca
     8521 caggtgcgcc agttcgagaa gggcctgctc tacgtgcagg gccgcttcgc tggcatcctc
     8581 gagccgggtc gtcacgcgtt ctggaaccac ccgggtgcac gcatcacggt gaacgttctc
     8641 gacacgcgtg tgcagcagct gaagatcgag ggtcaggacc tcatgacgcg tgacaaggtc
     8701 acgctgcgcc tgacgctcac ggcagagtac gcaccggcgg atgcgccgac gacggtccac
     8761 gccgttgccg acgtgaagga ggcgctgtac ctcgccgttc agctcgctgc tcgcgagtac
     8821 gtcgcgggcg tcacgctcga cgagctgctc gagggtcgtg acgcgctcac gcgctacctc
     8881 gaggcgcagg tccagccacg cgccgagatg ttcggtgtgc gcctgcaccg cgtcggtgtg
     8941 aaggacgtga ttctgccggg cgagatgaag acactgctca acaaggtgat cgaggccgag
     9001 aaggcggctg cagcaaacgt gatcctccgc cgtgaggacg cggctgcaac gcgcaacatg
     9061 gctcagaccg ccaaggtgat cgcagagaac ccggtgctca tgcgcctgaa ggagctcgag
     9121 accatgaagg acatcgccga gaagatcgac gagatcaagc tcgtgatggg tatggacggt
     9181 ctcgccaaca tcctgccgct ggcaaacggc aagcacgact gaggccaata ggtgatcgcg
     9241 cgtcgtgagt ccgcgagcga cgcgcgcacc ctaggccgaa ggagtccgtt tcggacgagg
     9301 cataacggaa gctagccact tccacagcta ggtggccggt gcagcaatgc atcggccatc
     9361 tgtattttcg gcgctacttg acgacttcga cgcgcgacag ctcgcgcaga tcgccgccgc
     9421 gtccccacgt ctcgccattc ttcttgaaga cgatgaagcg cagcgtgccc tgacggttga
     9481 gcttcacgaa cggcatcgcg gccttcggat cgagatcctg cttctcgagt acgaggttcg
     9541 tgcccttgga gtccgtcgcg atcagcttct cgtacttcgt gacgccggcc ttctcgagga
     9601 tcgccgcgag tggccatccc atgccgccgt tctcgccggg cggaggttgc cgcggcatct
     9661 cgagcagctg cgtacccatc agcttcgtct ccttgccgct cgccttcacg acgagctcga
     9721 gcttcgacgg gtccatgccg ccgccgtcgc cttgctcgtt ctcgccgcgg ccggagttct
     9781 gcgcgagctt gatgtgcacg tcgtgcagat gatcctggcg catctgcggg gtgcccttct
     9841 tcgcgagctc gacgggatcg aagtagccga acgcgggcgc gccatcggcg ccgggaaaca
     9901 gcgcgggcac tagctccgga aatgtcgacg acggattgtt gatgtcgatg ggcttgggcg
     9961 agctcgcttc gagcgtgacg gtcttccact tgccgaggcg gcgagcgtcc gtcggcacga
    10021 gcgtatcgac gcgcggccac cctgcgactt gctcgggcgt cacctgcgcg acctgcgcgt
    10081 cgtcgacaaa gacgagcacg ttcttcgact cgggaaccgc ggcggatccg gcgctgcctg
    10141 cgctgccggt tgtcgcgggc ttgtcagcgg ctttgccgca cgcggcgacg gctactagga
    10201 ggacgacgag actgcggaca cccatgtgcc gcgagcgtat cgcgtcagtg gccgccggtc
    10261 gaaccggagc gaggcttcgc gttccagatg cgcagcgtga gcaggaagcc gacgatggcg
    10321 atcgggatca tcgcgatcgg ccagacgctc cagttcgcgc tcttggcgta ctccgggttc
    10381 ggcaccaggc agttcgcggc atcgcgcatt ttcgatgcgg gcagcgcatg accgtacacg
    10441 aacgattgga ccgtcgagcc gaggtagacg aagccatcga tgagaccgac tgcgacgccc
    10501 gtgttcttct tgccggcgaa gtcagcggat gccgttgcgg tgagcatgcc gtggacgcca
    10561 atgatcgcca tcgacatgaa cgcgacgacc cagccgatcg acatcggcat gtcgagcagc
    10621 acgaggatca tgatcgagcc gccgatcatg atgccgtaga ggacagctga caccgggctt
    10681 cggcgcgact ggaacaagtg atcgctgagc gcgcccgcga acatgccgcc ggtgattccg
    10741 gcgatgcacg acaccatgcc ccagttcttg aagacgaagg agctggagac gccgacgcct
    10801 ttgccgaagt cagggcccca ctgcaggatc gcctggcgca agaagcccga gcacagctcg
    10861 atgagcgcga tcgtgagcaa cacgggatgg ctgatcagct tccacaacac gaaccggatc
    10921 tgaccaagcc agacccagaa cgtgagcttc ggcgtgtcct cgtcggcctt gtttgtcgcg
    10981 tcgcccagat cgaagttcgg atgtcctgcg tcagccggct cgtttcgcac gaacacgaat
    11041 gacaggacga agaacacgaa caagatcatc gccggcacga ggaagatcca ttggatcgag
    11101 tagttctctt tgatgcggcc gatgacgtcg aacgcgaaat agaggccgag cgagatcaga
    11161 attccgaaga tgccgccgaa cgtgccgcgc tcgcgcaggt ggaaccacgc ggcgttgacc
    11221 ttcacgatcg agacggcgcc gaagctctgg aagtacatgt tcatcgcgaa cgcggcggtg
    11281 aacgcggtcg gcacggacat gccgaacgtg ttcttcgtcg ccgccatgta ccccatccac
    11341 agatttgcga cgccagcgcc tgccgcggag atgaggatgg tcgcgcgacc tccccatcga
    11401 tcagtgagcg ggccattgat caagaatgcg agcccgtaca ccgccgagcc gatgccgaac
    11461 acggtgccgt actcggcctc ggtcagaccg acgccttgaa acgacttgat gttgtagcga
    11521 gcgaagtaga ggaacgagta cgtgaggcca agcggtaacc agttcagcaa ccggcgactg
    11581 cgaaacgccg gcgtgtgccc gagctcaacc ttcggtagtc gccacagcac gaggacgatc
    11641 accgcgagca agatgccgat cggtaagagg tcgtccttag ctacctgagt aaacgtcttc
    11701 ttgacgtgcg gcttcttcgg cggaaccgga cacgcgtcct tcgtctcgac gaccttcggc
    11761 gcatcggccg caggctgtgt gaccgctgcg ctccccgcgg cagacgcgct ctgcgagggc
    11821 tgcgcatcag ccgacggcga atgcgaaaac caaatgcagg ctccggcgac cagctcgagc
    11881 accacgacga gcgcgagcgt ccgtccagcc acccttggtt tcgacatcgg cgccgacgtt
    11941 actggatatc gacaggtctg tgaacgggtt cctccgacgg agatggcctg aaaattcaga
    12001 gcccttccga cggaagctcc acgaggtgag caaaaactat tttgctttga ggtcgcgtga
    12061 aaacgtaacg accatcgcgc gattaaaaat tacagagacg ttttcacagc gcatgaccgc
    12121 actcggagaa agttgtggcg acgtaatgtg ccgtcgtgtc ggataaaatt ggcaagtgac
    12181 tcgaccgcgc agcgacgcta cagagagcgc acatgactca acggatcacg ctctccatcg
    12241 ctctcgcggc ttctcttctg acagccgcct gcgcgatcga tgacaaggta gacccgggag
    12301 ccggcggcga gggcgagggc tcggattact ccggcagtgg cagcgaggac gtccccatgg
    12361 accccggcta cggcggtggc atggatatga cggaccctga cgtcaacgtt cagcccacgt
    12421 accctacggc gcatccgcgc atctacgtca atgcgaatag ggcgcgcctc caagcggctc
    12481 tcgcagcgaa caccacgcct gcaagccgtt tccgtacgac ggtcgataac cagatcaacg
    12541 gtgcgaacta ctgggagttc cgcgcgtgga acatcgctct catgggtcag ctcagtggcg
    12601 acccgaagta ctgcacgaag gcgatcgcgc aggtcgatgc atttgtcgcg agtgaggagc
    12661 ccaagatcgc tgcgggtacg cagccgacgg ttgcgggcaa tagctacctc tatgtcggtg
    12721 actacctcgg tgacctcgcg ctgacgtacg actggtgctt cgatggcctg accgcatcgc
    12781 agaagacgcg ctggcagacg tacgcagaca ccacgctgtg gaacctgtgg catccgacgc
    12841 aggcgaagtg gggcggcaag acgctcgcgt ggaccggctg gtcgacgaac aaccccggcg
    12901 acaactatta ctactcgttc atgcgcgcga cgatgctcgt gggtctcgcg atgaagggcg
    12961 agacggcgca gggcgacacg tgggtgaaca cgttccgcga cgagaagatc ctcggccagc
    13021 tcgttccgat gttcgacaag gatctgctcg gtggcggctc gcgtgaggga accgcgtacg
    13081 gcgtttcgat gcgcggcctc ttccacctct atgacatgtg gcacgacacg accggcgaga
    13141 gcctgaagtc gaagacgacg catgcgcgcc gctcgatgcg cctgttcgtt caccagacga
    13201 tgccgacgct cgaccgcttc gtcgactcgg gcgatcagcc gcgcgacgcc gctgcgatgt
    13261 tcttcgacta ccagcgcaac tatctgcagg agctcgtgca gatgtatccg aacgactcgg
    13321 tcgcgccgaa ggcgaaggca ctgctcgagg gctcgacggt cacgcagctc gcgcgtcccg
    13381 agacgctcgt gttcgatttc ctctacgaca tgaacaatgt gacgtcgcag ccgctcggtg
    13441 gcatgggcac gtcgctctac gccccgggca tcggtcaggt gtactcgcgc tcgggttggg
    13501 acaaggcagc gacgtgggtg aacttcaccg cgggcgcgta tgccgaatcg catcagcatc
    13561 aggaccaggg ccagctcgtc atcttcaaag agggttggat gatggccgac gcaggtctcg
    13621 gttcgcgcaa cggcatcatt caggacacgc cggctcactc gatcgttcgc atcgacagcg
    13681 gctcgacgcc cgtcaagcaa atggtcaaca ccgaatcgaa gctggttggg cttcatggtg
    13741 ctgacacgtg gctctatgca gctgcggact tgacgccggc gtacggtggc aacgcagcgg
    13801 ttcagaaggt ccagcgcgag tttgtcttcc tgaagccgaa cgtcatcgtc gtgtacgacc
    13861 gcgtcgcgac ggcgtcgggt acctcgcaga cgtggcagct cgcgtcgccg accgctccgt
    13921 cgatctcggg cgcgacgacg acggttgcgg gtgcgcatcc gatgacagtg cgccgcctgg
    13981 ttccggcgtc ggcaacggcc gccgtcacga acatgacgag cgtgacctcg accgcgggta
    14041 gttatctctc gggctatcgc ctcgacgaga aggttgcggg tggcgaccag cgttacctcc
    14101 acgtcatttc gctcgacggc gcggcgacga acgcgacggt gtcgggtgac tcgggcgtga
    14161 cggtctcgct gtccgacggc agcaccgcca ccgtgagctt caaccgcgac acgatcggcg
    14221 cgacgcttgc ctggggctcg gtctccgaga cgctcggcgc cggtgtcgac atgctcgccc
    14281 agtagcgtcg ccgcaagttg cgcgagtgac agcagcgcct gccccgtgat aagcggggac
    14341 gttcatgccg ctgtggatgc gcacgacgct gaggctctcg gtcgttgtcc tcgtggcagc
    14401 ggccgcttcc attttcggcc tcgtcctcgt ccggcaggtg atcgactacg actcgctgcg
    14461 cgcgtcgagc gattcgctcg gcacgttcgt gcagacgctc ggcggcatct acgccgtgct
    14521 gctcgcgttc gtcgtgatcg tcgtgtgggg ccagttcaac gatgcgcgcg gctacgtcga
    14581 gcgcgaggcc aacgcgctgg tcgatctgca tcgcaccgca agcggactgc cggtgcactc
    14641 gcggacgatc atccagaagg agctgcgcga atacgtcgat gcggtgatta ccgacgagtg
    14701 gcaggcgatg tccaaaggcg atgtccacac gatcgaacgc attaccggcc gcctcgatca
    14761 tgtctggacc gcgatccaca cgtgcgagcc gcgcaacgaa tgccagcaga cgatgcactc
    14821 cgaagtgctg tcgcgcttca acgacctcac ggagcatcgg acaaaccgtc tcaacagcgc
    14881 aggcgcgcgt atcccgatcg cgctcaacgt gctgctctat acgggcgccg tcatcatgat
    14941 cggcgcggtc tatctactcg cgatcgatcg gttctggctg cacgcgctcg tcacgggcct
    15001 gatgtcgggc gcggtcgcgc acatcttgtt cctcatctac gacctcgatc acgagttcgc
    15061 cggcgactgg caggtcgaga agaccgcgtt cgttcgggcg cgccgcgcct tcgagcgcac
    15121 cgaagagctg cacacgcacg agccgctcta gaccctagtc cgcgacctgg acgttcgagt
    15181 gcgcggtgag ccacgcacgc agaggaagtc ccttcggtgg atcgtccgac tcgtcgagtg
    15241 tccacacgcg agcgcgattg tcctcggagc cggacacgag gcggccatca ttcgtgaacg
    15301 cgacattctt cacggcgaac acgtggccat gaaattcgtt gcgcagcttg ccgtccggca
    15361 gcgtccacac gcgaacgacg ccgtcgatgc cggcggaagc gacgagcgat ccatccgcgc
    15421 tcatcgcgat gtccttcacg cccgcgttgt tgccttcgag ctcgagctcg gtgccctcgg
    15481 gaatcgcatg caggatcacg cgcgagtcgc cgccggcgga gacggcatgc gatccgtcgg
    15541 gcgtgaagat cactttgcgc gtgacgtccg tgtgcctgcc gagcgtcttc acgcgcgcgc
    15601 catccgcggt ccacaacgtg acgagcccat cctcgtcgcc ggcgatcagc cacttcttgt
    15661 cgggcgaaag cgcgaagcac cgcagctcgg cagcatgcgc gggcttcatc gcgcctttgc
    15721 cggtcacgac atcgacgcgg cccatgttgc cgtcgtaccc accgacgaag acggtgtggt
    15781 cgtcgtacca gatcacgggg cgaagaccgt ggttgtgccg caagagctgc ttgcccgtcg
    15841 cgctcgtgac gtcccagacg tagagcgagc cgtcctcgct cgtcgatgcg agcttcgtgt
    15901 cgtcgggcga gaacgcgacg ccgcgcacgg tgttgctatg tcccgacagc ttgcggccgg
    15961 tgcccgtcgc gagctcccac agccatacgt cgtgatccgt gcccgccgac gcgagccact
    16021 tgccgtcctt gctcatggcc aacgtctcga tcggcccatt gtgcccacgc agctcgacgg
    16081 acgtccgatc gtcgaggttc caccagcgga tcgtcgagtc gtcgctgccg gtcgcgacat
    16141 gcttgtgatc cggcgacacc actacgagct cgacatcctc cgtatgccca cggagctcct
    16201 gcgccaagcc atcgcgcgcc gcctcgacgg caatcgcgtg cgtcgactcc caccgaaatc
    16261 cttcgtcgcc catcaggccg agccatgcga ccgcccgact cggatcgata tcgagtgatt
    16321 gctgcgccat cttcagcttg tcgcgattgt tcgcgtcgcg gagattcaag ttcgcttgca
    16381 tcgcctcgtt cttcgcggcg accgcttgct tcgtctgctc ctgcgcgacg ttcttcgcgc
    16441 gcatcacgct caaaaacgca tagacgccga tgctcgcgag caacaccacg gccgccgcac
    16501 taatcccgac cgtcacgcga tgccgccaga tccaccgctt cacgagctcg ccgagcgagt
    16561 acgcatgcga cgcgagcagc ttgccggccg caaaccgcct cagctcctcc gcgagctcct
    16621 tcgcgctcgc aatgcgcttg ctaggctcgt gcgccatcgc gtgctcgacg agctcggcca
    16681 gctcgtgcgg caattccgcc tcgcgctccg caatgggcac gtgcttgcca gcggccacct
    16741 gcgcgagcac ttcgtccgtc gtcttgccgc gatgcggtgc ctcgccggtc agaatgttgt
    16801 agagcaacgc gccgagcgca tagatgtcgc tgcgctcgtc gctcggctcg ccgcgcgcct
    16861 gctcaggtgc catgtacgac ggcgttccca acaccgcgcc gacttgcgtc aggttcgcat
    16921 cgctcgcgcg ctcgtcggct tcgacacgct catcaggcaa gggcgtcgcc ggtcgtcgct
    16981 gctttcgccg cgcacccgaa tcgctcgcga gctgcccatc ctcgtcgcga atctctttcg
    17041 ccaagcccca gtcgatcacg accgtctcgc cgaacggacc gatcagcaca ttcgacggtt
    17101 tcaagtcgcg atgcagcacg ctctcgctat gcgcata
//
LOCUS       DATLBZ010000421         5783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9948399, whole genome shotgun sequence.
ACCESSION   DATLBZ010000421 DATLBZ010000000
VERSION     DATLBZ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9948399"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="VMZ53_21435"
     CDS             complement(<1..1763)
                     /locus_tag="VMZ53_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HUS31094.1"
                     /translation="MGRKEDALEYHARGRKGKLEVVPTKPLVSQIDLSLAYTPGVAEP
                     CLEIHADEEKSWEYTARGNLVAVISNGTAVLGLGDIGPAAGKPVMEGKACLFKKFADI
                     DVFDLEVAEKDVDKFCDIVAALEPTFGGINLEDISAPACFEIEEKLRKRMRIPVFHDD
                     QHGTAIISGAGLLNAAELAQKKLENIKLVVSGAGAGAIACVEFFCTLGMRRENVVLVD
                     SKGVIHAGRTDLNRQKQKFAIPDDGRRTLADAMVGADMFMGLSVAGTVKPEMLKGMAE
                     RPIIFALANPDPEISYPDAIAARPDALVATGRSDYPNQVNNVLGFPYIFRGALDCRAS
                     TISEAMKLAAARSLAELTREPVPDTVIMAYGGKTLKMGPDYFIPKPFDPRALWWVAPA
                     VAQAAMESGVARSTFDIAEYKERLMSKGSNAAYSIMRTIGREARREPKRIVYPHAGNP
                     RLLRAVQQIVDEGIAKPVLLGRPAEIRQMCEEMSLDMLSRGVEVIDPRSNRRQEYADR
                     FFELRQRKGITLEAAQALLQKSDYYAALMVDCGDADGVVTGLRLNYPETVRPLLEIFG
                     LMPGVKVAVGMYMMVLQNSVK"
     gene            1797..2477
                     /locus_tag="VMZ53_21440"
     CDS             1797..2477
                     /locus_tag="VMZ53_21440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31095.1"
                     /translation="MGWLGRLMAGGEGELGWDDLIRRIVDAIAPLKVYGPKGEVTFPD
                     EVIVRITVAEASQTVIQSFVDKPELDHEVGAALANRCDVAPQALPTREYVVSAADRIS
                     IVATQGAPRTWALSVVGGDRDGAALPLPSSWSELAFGRGAANNDLAVCEKTEFVSRRA
                     GKLYRAGNQLEVASLDQGDELIVRRPSGETIRPARTARGRAVVKPDDTIELGDGRGNL
                     VKLLVTRS"
     gene            2474..3262
                     /locus_tag="VMZ53_21445"
     CDS             2474..3262
                     /locus_tag="VMZ53_21445"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HUS31096.1"
                     /translation="MKIRTDGTNQFRIDFKGTQIDPFGIAQAIATTMRECKVRSAAGH
                     PLVWNEYRMILSQPDYESIRPLQAPLERDLRKVLADEARTRKAELVGEMRVTVVPDEA
                     DELPAGTGVVRVAFVPTDKLQSPRSGELTMRFDGFAVAGEIVAKPTDTVIVADTSSTA
                     AACTLRWGGSGEARLVAGTTMIAGRPHAESPPHFIALTGAAATVNKQHFWIAATASSI
                     RVGRYAKANPVHVNGQLVEAGADIEATLPAEISLSRGDLVIFVK"
     gene            complement(3292..3759)
                     /locus_tag="VMZ53_21450"
     CDS             complement(3292..3759)
                     /locus_tag="VMZ53_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31097.1"
                     /translation="MNHGERNGELGVRSLGRFVGRLLGDELAEIHASGAPSAELFAIT
                     SRPNEGFAISRHADIETAERACLAPGVVDRAVIRIGNEDIVMLVASGASAAAECMLGF
                     VADDDTGIATSHLHVEGELVAWRQQLVSSIVEGMASRRDIRIGQLGDFGDDRA"
     gene            4028..>5783
                     /locus_tag="VMZ53_21455"
     CDS             4028..>5783
                     /locus_tag="VMZ53_21455"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HUS31098.1"
                     /translation="MKRWLALEVAFLAGCALIAWWIGSHGTSAWLGRVARLKLAMPGG
                     GAGSGAGGAGGGVIAAAAEHAVRSAARTATLTLIAGIVMLAIGRVVGARRAGALPIAV
                     PWLVPAAVVATLLGFAVHVSTVEVSHGVAVMPSAVGFAQGFLVGAIAAAAVLVAPVDL
                     AELASRFRKPIAIGIAGIFVALAVAGSGPAGSGTRINLGPLQPIELVKPLVVIFLGAY
                     FGARAPKLRWHRQRVLGLRWPRVDLLLPAIGVLVMIVLGLRLVGDLGPVLLLAFVFLG
                     MFFLASRATGWVVVAFAIIAFVLLLLALAPGLAGSGTVKTRLVMWRDPWHNGLPNGHQ
                     LGEGLWAFAAGGWNGQGLGHATTPLVPAGKTDLVMATFVEQCGALGLVAYCGATASIV
                     LGALRVAASTRTAERTLIAGGIGILVLAQWIVIQAGTLGYLPLTGIVVPFMSAGRSSM
                     AVFVVLVAIVVRLAADGRARVDSTELAELHGSARGVSKVAMALAALALIAGVAAATLG
                     RGPESGHGIRVRLADGTRVTRMNPRLVKIAEHLRRGPIVDRNGVVLAESPTPGTRRYP
                     LGNALGTLLGIADPKVLLP"
BASE COUNT          881 a         1937 c         1954 g         1011 t
ORIGIN      
        1 aacttcacgc tgttctgcag caccatcatg tacatgccga cggcgacctt cacgccgggc
       61 atgaggccga agatctcgag cagcgggcgg acggtctccg gatagttgag acgcaagccc
      121 gtgacgacac cgtcggcgtc gccgcaatcg accatgagcg ctgcgtagta gtcgctcttt
      181 tgcagcaacg cctgcgcggc ctcgagtgtg atgcccttgc gctggcgcag ctcgaagaag
      241 cggtccgcgt attcctgcct gcgattgctg cgtggatcga tcacctcgac gccacgcgac
      301 aacatgtcga gcgacatctc ctcgcacatc tggcggatct ccgcgggacg tccgagcagc
      361 actggcttcg cgattccctc gtcgacgatc tgctgcacgg cgcgcagcaa gcgagggttg
      421 cccgcgtgcg gatacacgat gcgcttcggc tcgcggcgcg cttcgcgtcc gatcgtgcgc
      481 atgatcgagt acgcggcgtt agagcccttc gacatcagcc gctctttgta ctcggcgata
      541 tcgaacgtgc tgcgcgcgac gcccgactcc atcgcagcct gcgcgaccgc cggtgcgacc
      601 caccacagcg cgcgcggatc gaacggcttc gggatgaagt agtcggggcc catcttcagc
      661 gtcttcccgc cgtacgccat gatgacagtg tcgggcacgg gctcgcgcgt gagctccgcg
      721 agtgagcgcg cagcggcgag cttcatcgcc tcggagatcg tcgacgcgcg gcagtcgagc
      781 gcgccgcgga agatgtacgg gaagccgagg acgttgttga cctgattggg atagtcgctg
      841 cggcccgtcg cgacgagtgc atcggggcgc gccgcgatcg catcgggata gctgatctcc
      901 ggatcgggat tcgcaagcgc gaagatgatc ggccgctcgg ccatgccctt cagcatctcg
      961 ggcttcaccg tgcccgcgac cgacagcccc atgaacatgt cggcgccgac catggcgtcg
     1021 gcgagcgtgc ggcggccgtc gtcggggatc gcgaacttct gcttctggcg attcagatcg
     1081 gtgcggcccg catggatgac gcccttgctg tcgacgagca cgacgttctc gcgccgcatg
     1141 ccgagcgtgc agaaaaactc gacgcacgcg atcgcgccgg cacctgcgcc ggacacgacg
     1201 agcttgatgt tctcgagctt cttttgcgcg agctccgccg cgttcaacag gcccgcgccc
     1261 gagatgatcg cggttccatg ctgatcgtca tggaacacgg ggatgcgcat gcgcttgcgc
     1321 agcttctctt cgatctcgaa gcacgccggc gccgaaatat cctcgaggtt gatgccgccg
     1381 aaggtaggct cgagcgcggc gacgatgtcg cagaacttgt cgacgtcctt ctcggcgacc
     1441 tcgagatcga acacgtcgat atcggcaaac ttcttgaaga ggcaggcctt gccttccatg
     1501 acgggcttgc cggccgccgg gccgatatcg cccaggccga gcacggccgt cccattcgag
     1561 atgactgcca ccaagttgcc gcgcgccgtg tactcccagc tcttttcctc gtcggcgtgg
     1621 atctcgagac agggctccgc gacgccgggc gtgtacgcga gcgacaggtc gatctggctc
     1681 acgagcggct tggtcgggac aacctcgagt tttcccttcc gtccgcgtgc gtgatactcc
     1741 agcgcgtctt ccttgcgacc catgcggcca acctagcaaa ttgctagatt gtttgcgtgg
     1801 gatggctcgg acgactcatg gcaggcggcg aaggcgagct cggctgggat gacctcatcc
     1861 gccggatcgt cgacgcaatc gcgcctctca aggtctacgg ccccaaaggc gaagtgacat
     1921 ttcccgacga agtcatcgtc cgcatcacgg tcgccgaagc ctcgcagacg gtgatccaat
     1981 ccttcgtcga caaacccgag ctcgaccacg aggtcggcgc cgcgctcgcg aatcgttgcg
     2041 acgtcgcgcc gcaagcgctg ccgacacgcg agtacgtcgt ctccgccgcc gaccgcatct
     2101 cgatcgtcgc gacgcagggt gcgccgcgca cgtgggcgct ctcggtcgtc ggtggcgacc
     2161 gcgacggcgc cgctctgcct ctcccctcat cgtggagcga gctcgcattc ggccgtggtg
     2221 ccgcgaacaa cgacctcgcg gtctgcgaaa agacggagtt cgtctcgcgg cgcgcgggca
     2281 agctctaccg cgccggcaac cagctcgagg tcgcgtccct cgatcaaggc gacgagctga
     2341 tcgtccgccg cccctccggc gagacgatcc gccccgcgcg caccgcccgc ggccgcgccg
     2401 tcgtcaaacc cgacgacacg atcgagctcg gcgacggtcg cggcaatctc gtcaagctcc
     2461 tggtgacgcg ctcatgaaga tccgcaccga cggaacgaac caattccgca tcgacttcaa
     2521 aggcacgcag atcgatccat tcgggatcgc gcaagcgatc gcgaccacga tgcgcgagtg
     2581 caaagtgcgc tcggccgcag gccacccgct cgtgtggaac gagtaccgca tgatcctctc
     2641 gcagcccgac tacgaatcca tccgcccgct gcaagcgccg ctcgagcgcg acctacgcaa
     2701 ggtcctcgcc gacgaagcgc gcacgcgcaa agccgagctc gtcggagaaa tgcgcgtgac
     2761 cgtcgtcccc gacgaagcag acgagctgcc cgccggcacc ggcgtcgtgc gcgtcgcgtt
     2821 cgtgccgacc gacaagctgc agtcgccacg ctcgggcgag ctgacaatgc gcttcgacgg
     2881 cttcgcggtc gcgggtgaaa tcgtcgcgaa gccgacggac accgtcatcg tcgcagacac
     2941 atcgagcacc gccgccgcat gcacgctgcg ctggggtggt tcgggcgaag cgcgcctcgt
     3001 cgcgggcacg acgatgatcg caggtcgccc gcacgccgaa tcgccgccgc acttcatcgc
     3061 gctcaccggc gccgccgcca ccgtcaacaa gcagcacttc tggatcgcgg cgacagcatc
     3121 atcgatccgc gtcggtcgct acgcgaaagc gaatcccgtg cacgtcaacg gtcagctcgt
     3181 cgaggcgggt gccgacatcg aggccacact gcccgccgaa atctcgctgt cgcgcggcga
     3241 cctcgtcatc ttcgtgaagt agcgccgcta ccgacgcggt agtcgtcgtc gctatgcgcg
     3301 gtcgtcgccg aaatcgccga gctggccgat ccgaatatcg cgacgagacg ccatcccctc
     3361 gacgatcgac gacacgagct gctgccgcca cgcgacgagc tcgccttcga cgtgcaggtg
     3421 gctcgtcgcg atgcccgtat cgtcgtcggc gacaaagccg agcatgcact ccgccgccgc
     3481 gctcgcgccc gacgcaacga gcatcacgat gtcctcgttg ccaatgcgaa tcaccgcgcg
     3541 atcgacaacg ccgggcgcca aacacgcgcg ctccgcggtc tcgatgtccg catgccggct
     3601 gatcgcgaag ccctcgttcg gccgcgacgt gatcgcgaac agctccgccg acggcgcgcc
     3661 gctcgcatgg atctccgcca gctcgtcgcc gagcaaccgg ccgacgaatc gccccagact
     3721 ccgaaccccg agctccccat tgcgctcacc gtgattcacg gcttctacct actgcgaccc
     3781 gcgaaacatg ggacgccaaa gttgtaacag ttcgcgccac gcgcccgtcg ctgtagcgcg
     3841 cgcgatcccc gttgtgcaac gcattgctca gcaagggcag aaaatattcc gcgcgtgccg
     3901 tcacacgcgt cgcgcccacg tgggcacatc gcgccgatga agtcttgtgc gctgaacact
     3961 gtcctccgat cccgttcgtg agggatcgtt tccaggtgct cggtgcatgt ttgcgaggtg
     4021 gcgtcgcgtg aagcggtggc tcgcgctcga ggttgcgttc ctcgcgggtt gcgcactcat
     4081 cgcgtggtgg atcggatcgc acgggacgtc ggcgtggctc ggacgcgttg cgcggttgaa
     4141 gctcgcgatg cccggaggcg gcgcgggtag cggcgccggc ggcgcgggtg gtggcgtcat
     4201 cgcggcagcg gcggagcacg cggtccggtc ggcagcgcgc acggcgacgc tcacgctcat
     4261 cgccgggatc gtgatgctgg caatcgggcg agtggtgggt gcgcggcgtg cgggcgcgtt
     4321 gccgattgcg gtcccgtggc tggtgccggc ggccgtcgtg gcgacgttgc tcgggtttgc
     4381 cgtgcatgtc tcgaccgtcg aggtttcgca cggcgtggcg gtcatgccgt cggcggttgg
     4441 gttcgcgcag ggctttttgg tcggtgcgat tgcggcggcg gccgtgctcg ttgcgccggt
     4501 ggatctggcg gagctcgcat cgcggtttcg gaaaccgatc gcgatcggta ttgcgggcat
     4561 ctttgtcgcg ctcgcggttg cgggatcggg acccgcgggg agtgggacgc gcatcaactt
     4621 ggggccgctg cagcccatcg agctcgtgaa gccgttggtc gtgatctttt tgggggcgta
     4681 ctttggcgcg cgcgcgccga agctgcggtg gcaccggcag cgagtgctcg ggttgcggtg
     4741 gccgcgcgtc gatctgctgc tgcctgcgat cggtgtgctc gtgatgatcg tgcttgggct
     4801 gcggctcgtc ggagatctgg ggccggtctt gctgcttgcc tttgtgttct tggggatgtt
     4861 ctttctggcg agccgggcga ctgggtgggt cgtcgtcgcg ttcgccatca tcgcgttcgt
     4921 gttgctgctg ctcgcgcttg cgccgggact ggcgggcagc ggcacggtga agacgcgact
     4981 cgtgatgtgg cgcgacccgt ggcacaacgg gctgccgaac ggtcaccagc tcggcgaggg
     5041 gctgtgggca tttgcagccg gtggctggaa cggtcagggc ctggggcacg cgacgacgcc
     5101 gctcgttccc gcgggcaaga ccgacttggt gatggcgacg ttcgtcgagc agtgcggcgc
     5161 gctcgggctc gtcgcgtatt gcggcgcgac ggcgtcgatc gtgctcgggg cgctgcgtgt
     5221 tgccgcgagc acgcggacgg cggagcggac actcattgcg ggcggcatcg gcattctcgt
     5281 gctcgcgcag tggatcgtga ttcaagccgg cacgctcggg tacttgccgc tcaccggcat
     5341 cgtcgtgccg ttcatgtcgg cgggacgcag cagcatggcg gtgttcgtcg tgctcgtcgc
     5401 gatcgtcgtg cggcttgcgg cggatgggcg cgcgcgggtc gattcgacgg agctggcgga
     5461 gctgcacggt tcggcgcgtg gcgtatcgaa agtcgcgatg gcgcttgcgg cgttggcgct
     5521 gatcgccggc gttgcggcag cgacgctcgg ccgcggtccg gaaagcggac atggcatccg
     5581 cgtgcggctc gccgatggca cgcgcgtcac acgcatgaat ccgcgcctcg tgaaaatcgc
     5641 cgagcatctg cggcgcggcc ccatcgtcga tcgcaatggc gtcgtcctcg cggagagccc
     5701 gactccaggc acgcgccggt atccgctcgg caacgcgctc ggcacgttac tcggcatcgc
     5761 cgacccgaaa gttctgctgc cca
//
LOCUS       DATLBZ010000422         3089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9950607, whole genome shotgun sequence.
ACCESSION   DATLBZ010000422 DATLBZ010000000
VERSION     DATLBZ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9950607"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VMZ53_21460"
     CDS             <1..549
                     /locus_tag="VMZ53_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31099.1"
                     /translation="NYLEKMTLTTGERMIETAGIAAQQLWFDQARMMWVPGSSRAFSN
                     FMIDTTDMTDMMTHEEWLSIPETERSFAQPLPAAKIFNTVFVNELQIELGMEKAYVER
                     LKTLEAQLAMSPNDANVKAQLEGAKFVWAQYTGAMKVLTELKGANVIDRLKRAGAPSG
                     ITWTAPSDSKGNIALKILADRD"
     gene            565..1701
                     /locus_tag="VMZ53_21465"
     CDS             565..1701
                     /locus_tag="VMZ53_21465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HUS31100.1"
                     /translation="MVNSKPRVVILGAGFGGLELSTTLSEALSGSIDITLIDKSDHFI
                     FGFSKLDVMFGHKAADAVRLPYSAFVKPGVRLLRETVTAIDPVTRRVTTNAGTHDADY
                     LVIALGADYDVSETPGITLGENEFYSVAGATHLRDVLPTFKRGHAVIGVCNAPFKCPP
                     APSECALMLHDYLSTRGVRDACTITLVLPLSAPVPPSPDTSKALVAAFAERNIKLILT
                     RKVTAVRGKTVVLDDASELPCDLFLGVPRNRAPDVVVATGMTEGGWVTIDPRTLETKH
                     ANVWALGDLANTGAPKAGVFAEAAAKTVAARIMSRLRGEDPTAKNPGMGACYIEFGAG
                     LVARVDADFFSGPQPTGVYNAPSEEIAESKATWSASLRARWFGM"
     gene            1733..>3089
                     /locus_tag="VMZ53_21470"
     CDS             1733..>3089
                     /locus_tag="VMZ53_21470"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31101.1"
                     /translation="MRRPRPEPAGRDDQLVRVRGAREHNLKNIDLELPRNALVVFTGV
                     SGSGKSSLAFGTLYAEAQRRYLESVSPYARRLFDQMPVPDVDEIEGLPPAVALQQQRG
                     SPTTRSSVGTVTTLSNLLRMLYSRAGNYPRNAQGILYADAFSPNTPDGACPTCSGLGR
                     IYEVTERSMVPDPTKTIRERAIAAWPLAWGGQNLRDILVALHIDVDIPWQDLPKKTRD
                     WILFTDEQPEVPVYAGLTPAETKRAQKRREEPSYMGTFSSARRHVFYTFTNTQSAMMK
                     RRVMQYMIASECNACDGKRLKPASLAVKFAGLDIAELSRLPLKQLATVMEPYATGALV
                     IESAVEHPEKAVVAQRIAEDAVARLRVLLELGLGYLTLERNTPSLSPGELQRLRLATQ
                     VRSNLFGVVYVLDEPSAGLHPADTEALLAALDRLKAQGNSLFVVEHDLDVIRHADWIV
                     DV"
BASE COUNT          571 a         1002 c         1014 g          502 t
ORIGIN      
        1 aactacctcg agaagatgac gctgacgacc ggcgagcgaa tgatcgagac cgccggcatc
       61 gccgcgcagc agctctggtt cgatcaagcg cgcatgatgt gggtgccggg ctcgagccgc
      121 gcattctcga acttcatgat cgacacgacc gacatgaccg acatgatgac gcacgaagag
      181 tggctctcga tccccgagac cgagcgctcg ttcgcgcagc cgctgcccgc ggcgaagatc
      241 ttcaacacgg tgttcgtcaa cgagctgcag atcgagctcg gcatggagaa ggcgtacgtc
      301 gagaggctca agacgctcga agcgcagctc gcgatgtcgc cgaacgacgc gaacgtcaaa
      361 gcgcagctcg aaggcgcgaa gttcgtgtgg gcgcaataca cgggcgcgat gaaggtactg
      421 accgagctca agggcgcgaa cgtcatcgac cgcctcaagc gcgccggcgc accgtcggga
      481 atcacgtgga cggcaccgtc ggactcgaag ggaaacatcg cgctcaagat cctcgccgac
      541 cgcgactagg tgtatgacgg ccggatggtg aattcgaagc cgcgtgtcgt catcctaggt
      601 gcagggtttg gtgggctgga gctgagcacg acgctgtccg aagcgctgag cggatccatc
      661 gacatcacac tgatcgataa gtccgatcac ttcatcttcg gcttctcgaa gctcgacgtg
      721 atgttcggtc acaaagcggc cgatgcggtc cggttgccgt acagcgcgtt cgtgaagccg
      781 ggcgtcagac tgcttcgcga gaccgtcacc gcgatcgatc cagtgacccg ccgcgtgacg
      841 acgaacgcag ggacgcacga cgccgactat ctcgtgatcg cgctcggcgc cgactacgac
      901 gtcagcgaga cgcccggcat cacgctcggc gagaacgagt tctactctgt cgccggcgcc
      961 acgcatctgc gcgacgtgct tccgacgttc aaacgtggtc acgcggtgat cggcgtctgc
     1021 aacgcgccgt tcaagtgccc gcccgctccg agcgagtgcg cactcatgct gcacgactac
     1081 ctcagcacgc gcggcgtacg cgacgcttgc acgattacgc tcgtgcttcc gctctcggcg
     1141 ccagtgccgc catcgcccga tacgtcgaag gcgctcgtgg cagcgtttgc ggagcgcaac
     1201 atcaagctga tcctcacgcg caaggtcaca gccgtgcgcg gcaagaccgt ggtcctcgat
     1261 gacgccagcg agctgccctg cgatctgttc ctcggggtgc cgcgcaatcg cgccccagat
     1321 gttgtcgtcg cgacgggcat gaccgagggc ggctgggtca cgatcgatcc gcggacgctc
     1381 gagacgaagc acgcgaatgt ctgggcgctc ggcgacctcg caaataccgg agcaccgaag
     1441 gcgggcgtgt ttgcagaagc ggccgcgaag accgtcgctg cgcgcatcat gtcgcgcctg
     1501 cgcggtgagg atccgaccgc gaagaaccca ggcatgggcg cctgctatat cgagtttggc
     1561 gctggcctcg tggcgcgcgt cgacgcggac ttcttctcgg ggccgcaacc gacgggtgtg
     1621 tacaacgcgc cctccgaaga aattgcggaa tcgaaagcga cgtggagcgc ctcactgcgt
     1681 gcacgctggt tcggcatgta gatcggcgct aagcactaca tagatgtctg cgatgcggcg
     1741 cccgaggccc gagcccgctg gccgtgatga ccagctcgtg cgggtgcgcg gtgcacgcga
     1801 gcacaatcta aagaacatcg atctcgagct gccacgcaac gcgctcgtcg tgttcaccgg
     1861 cgtctccgga tcgggaaagt cctcgctcgc gttcggcacg ctctacgccg aggcgcagcg
     1921 ccgctatctc gaatcggtgt cgccgtacgc gcggcggctc ttcgatcaaa tgccggtgcc
     1981 cgacgtcgac gagatcgagg gcttgccgcc tgcggtcgcg ctgcagcaac aacgaggctc
     2041 accgacgacg cgctcgtcgg tcggcaccgt gacaacgctg tcgaacttgc tccgcatgct
     2101 gtactcgcgc gcgggaaatt atccgcgcaa cgcgcagggc atcctctacg ccgatgcgtt
     2161 ctcgccgaat acgccggacg gtgcgtgccc gacgtgcagc ggcctcggcc gcatctatga
     2221 agtcacggag cgatcgatgg ttccggatcc gacgaagacg atccgcgagc gcgcgatcgc
     2281 ggcgtggccg ctggcgtggg gcgggcaaaa cctgcgcgat atcctcgtcg cgctgcacat
     2341 cgacgtcgac attccgtggc aggacctgcc gaagaagacg cgcgactgga tcctgtttac
     2401 cgacgagcaa ccggaggtgc cggtctacgc gggactcacg ccggccgaga cgaagcgcgc
     2461 gcagaagcgg cgcgaagagc cgagctacat gggcacgttc tcgagcgcgc ggcgccacgt
     2521 gttctatacg ttcacgaaca cgcagtccgc gatgatgaag cgccgcgtga tgcagtacat
     2581 gatcgcgagc gagtgcaacg cgtgcgacgg caagcggctc aagcccgcgt cgctcgccgt
     2641 gaagttcgcg gggctcgaca tcgcggagct gtcgcgcctg ccgctgaagc aactcgcgac
     2701 ggtgatggag ccgtacgcga cgggcgcgct cgtaatcgaa tcagccgtcg agcaccccga
     2761 gaaagcggtt gtcgcacagc gcatcgccga ggatgcggtc gcgcggctgc gcgtgctgct
     2821 cgagctcggc ctcggctact tgacgctcga gcgcaacacg ccgtcgttgt cgccgggaga
     2881 gctgcagcgc cttcgcctcg caacgcaggt gcggtcgaac ttgttcggcg tcgtctacgt
     2941 gctcgacgag ccaagcgcgg gcctgcatcc cgcagatacc gaagcactgc tcgccgcact
     3001 cgatcgcttg aaggcgcagg gtaactcgct gttcgtcgtc gagcacgacc tcgacgtgat
     3061 tcgccacgcc gactggatcg tggacgttg
//
LOCUS       DATLBZ010000423         9963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9459312, whole genome shotgun sequence.
ACCESSION   DATLBZ010000423 DATLBZ010000000
VERSION     DATLBZ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9459312"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VMZ53_21475"
     CDS             <1..846
                     /locus_tag="VMZ53_21475"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HUS31102.1"
                     /translation="GAIQETAAVGVHGKIYVLGGFDSDDGIVALVQVFDASAGAWTSD
                     ATPLPRALHHINAVTDGDTIFVTGALSGVNFTPVGDTYSFTPGGAWQTRAAMPVGRER
                     GAAVTGIIDGKIYVAGGFRGGRAATLVDVYDPVADTWVARADLPVARDHACGGVLDGK
                     LVVAGGRDGSPSGPVPDVWAYDPAANTWTQRAPMPTGRGGMACGVIDGALYTTGGEGN
                     ANAASGVFDQVESYRGDQWTQHAPMPHPKHGVGGAVWDGALYLCGGANVQAFGAVATT
                     DILRP"
     gene            866..1546
                     /locus_tag="VMZ53_21480"
     CDS             866..1546
                     /locus_tag="VMZ53_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31103.1"
                     /translation="MRVHYLALVLACAACSKKSNDAPKATGSATPKTAEAPKPKSDAP
                     WDFDAAALQAKFQGAWVVKDHGYLGSSEAWEVKGDQVKIWDAKRDKETTATLNVMGPC
                     VYSMKEADGSSTTSHYVIDGDTLHMGLGDAGVKVGDKVIACMSNGVFVLDGGKCTFYL
                     DHFGKWSPEEGTCSLEGDTFKASNTSFKYEGSMPAHGSVFASDQLWNNPPPVKAASYD
                     EAKAKAKG"
     gene            1568..3967
                     /locus_tag="VMZ53_21485"
     CDS             1568..3967
                     /locus_tag="VMZ53_21485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31104.1"
                     /translation="MPTDDDDLGSAATIVGASTDAGAGTGAGNDATQMTQSSLPSSRD
                     SSPVIPPSVIADRYEILGLLGVGGMGRVYRAHDRALDEQVALKLLKRELVGVAGMLDR
                     FRQEVKLARRVTSPHVVRTFDLGEHAGEHFLTMELVEGRSLAQLLDDGQLAVDEILRI
                     SRAMAAGIAAAHATGVTHRDLKPDNVLVGKDGRVAITDFGIARAAAVSPRETVDGFVG
                     TPAYMAPEQVEGSDTIGPASDVYAFGAILFEMLAGRRPFVGSDLIAVAVARLREAPPD
                     PRQFRRMPDSLADLALRCLERDAAQRFANGGELAKALAAVTTDGPSTLVNQRPQRVPA
                     KTSRSVAIMPLRASGELAEVADGLSEEIVDALSMTRELRVRPLVSLRNAARAEQDSRE
                     LGKSLGVDVVVDGSIRKRGATAVRIAARVIGVEDGFQLWANHFDTSEDDLLAASDQVV
                     RAIANALTVELEVPQRAKLDPKVADVYLETKAKLRTNWLAGGLEDLFEPLEAAHRVAP
                     DDAGIISTLSLAHSRSAFYGGQGILARGRELADRATRLAPTSGEAWLALGVAALYGGD
                     MAAATHALIEACEHAPGLALAQALLGAVCLEAGHLNGALLHLEGAQSLDPFGPQLADL
                     PRAYVYAGREDEAMRMLEAQSTEYRLVSIARFKMWKGERYTINYTPSLPPTLPPNFSK
                     YARVAANVHRTAMISAEDRAVMDEIIAVENPRLSATRAQFVAEFYIFCGELDAAMGAI
                     EMAVKNGLQDHLWMQKCPLLDSLRDRPRFIDLATIVAARARVVLDVIDRLTTGEFRTA
                     S"
     gene            complement(3964..5184)
                     /locus_tag="VMZ53_21490"
     CDS             complement(3964..5184)
                     /locus_tag="VMZ53_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31105.1"
                     /translation="MSKDFSRRGLFKRAAVAAGAFSVGRIPGMSWLEREARADVAGEA
                     PALFIFNMIGGYNALFGSADSFQGTGAFGVTATNIKQVGTTKLFVDKSTLGTLSATTL
                     GHTASIGVDHGITSHPTARMSLLFDGTKSRLISLSKALGGTAAVRCVTIGNLMPVGTH
                     TAQGDVSLQQVRDLSTTIAVLGGVTSGNAPARGLAADGIAAAQAMSADAIAKNPTSAR
                     TFAESYPAASSQLKEATVALDYASMATAYGLTAGTGGSFATDVRNTTMQIMGAELMIR
                     AGANVVIANQGGWDSHGDNNGAEVRNKINGDGTMAALKVFTDRMMAETGRNVVTAIVG
                     DFSRSLPGSNHQANLTATVIGKYVKAGTTGRVNADVALPSGTPSIQGLWAYLAAALKC
                     PDQPFGTNPHALVL"
     gene            complement(5194..5784)
                     /locus_tag="VMZ53_21495"
     CDS             complement(5194..5784)
                     /locus_tag="VMZ53_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31106.1"
                     /translation="MFLILSLAGCVGQIDGSSGDDQTGDDDTQEVCEATRSYTGYGGK
                     TLEADRPTMKAGTDRLRMKPFAALAAEYAAALGLTGFDTAAYAATFGRPPARWYQEPA
                     ASANTIYAAFALAYDGCTRATATDAKFAAAPDATNAPLLCNETLRRAWHREPLQAETD
                     ACVNYTVNQTNASDAPRVRWAYSCAAVLTASGFLAY"
     gene            complement(5803..6759)
                     /locus_tag="VMZ53_21500"
     CDS             complement(5803..6759)
                     /locus_tag="VMZ53_21500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31107.1"
                     /translation="MRVRYLFGLGVPLAAVALWSSSGTSHSQTPADEAAKVRCATRLS
                     LSLTGKAPNAALLAAADPQAQIDTLLADPAFVEQFSRFVNSELNPEPAMAPAQDATYF
                     MAKYVLENQKPWHEMFDGKYDVQPVAAANGNPATAQIVADPNGLGYFRSRPWMVRYAG
                     NEEDGYRLSAAFRIQQNIIGLDVGAVTNAPGVDVSATGRMSGNCRGCHYDPYFALDKV
                     AKILSKRVDTTPITFSAPTEGPQTVLDGKTISNDGELVSALLESTDYKFRTCRMAFVF
                     LYGRPEASCESALFDKCIDAFTQTGDIRTALRTIAADPGYCE"
     gene            6911..7600
                     /locus_tag="VMZ53_21505"
     CDS             6911..7600
                     /locus_tag="VMZ53_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31108.1"
                     /translation="MRWTLVLVTGCWTSAPNAPARPPVIEPESTCMSLTAETLRNRDL
                     LPMKQAEGAPITKESPLRAVAMSGPYWNVAAACEDCERGDGSGRYLEIATGGRKLAIR
                     IGNKWWSTMLAINDTCALHFEPVVARDLVAPAPGDELFLQVTQDCAEPQDPFQEPDFQ
                     EHIVMCGVGRSQRPSCVEADFGVTGITPEWSEHTRLDLACDGIATFTGWHGEDGEGFT
                     SVHTRRQLVFP"
     gene            7624..>9963
                     /locus_tag="VMZ53_21510"
     CDS             7624..>9963
                     /locus_tag="VMZ53_21510"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31109.1"
                     /translation="MKPGELVEDRFELERVAGSGGMGTVYRAIDRTSGSPVALKVLRS
                     ASMDAAQRFTREVRILSQLRHPGIVRYIADGQTDGGELWLAMEWLEGESLNVRLARDG
                     LTAGESVELVRRVAEALGAAHEKGVIHRDLKPSNLFIAGGDLERVKVLDFGVARMTDN
                     SATRTGVMIGTPGYMAPEQAKGERNVGSRSDVFALGCLLYECLTGKPPFLGDNVMAVL
                     AKVLLEEAPRVGVVRPDLPEALDDLVARTLAKNPDHRPRDGAELAIELATLGTIHAAE
                     RPANQPSAALTATERRLLCVVLIDVGSREPVDSDATIQSLPSMMDNTLPSESSQPRDT
                     LAESMRPELKSLRETAESHGARLQRLVDGSLVVTLLGTGGTADQATQAARCALALKQQ
                     LPGIPMALATGRGVMAGRWPIGEAIDRASGLLLAARSASPHLVRVDEVTAGLLDTRFE
                     VGGDEAGLYLLGERDLVEARRTLLGKPTPCVGRDRELGVLAGLFDECVSEPMARAVII
                     TGSAGVGKSRVRYELLRRIKQREEPVETWIGRGDPLRTGSPFGMIAPALRRASGIADG
                     EPIEVRRQKLRARVMRHATTEVNRTTFFLGELMGVTFPDEESVELHSARQDMQLMADQ
                     TRRAFEHFLAMEASAQPVVIVLEDLHWGDLPTVKLIDSALRALPDLPWFVVAVARPEL
                     HELFPRMWAERGVQELRLDELTKRGSEKLVRSVLGDKANDALVARIVEQAGGNAFYLE
                     ELIRAVAEGKGETKIPETVLAVVQGRLERLEVDARRVLRA"
BASE COUNT         1673 a         3237 c         3391 g         1662 t
ORIGIN      
        1 ggcgcgattc aggagaccgc ggcggtcggc gtccacggca agatttacgt gctcgggggc
       61 tttgattcgg acgatggcat cgtcgcgctt gtccaggtct tcgacgccag cgctggcgcg
      121 tggacgagcg atgcgacacc actgcctcgc gcgcttcatc acatcaacgc cgtgaccgac
      181 ggagacacca tcttcgtcac cggcgcgctc agcggcgtca acttcacgcc ggtcggcgat
      241 acgtatagct tcacgccggg cggtgcgtgg cagacgcgcg cggcgatgcc ggtgggtcgc
      301 gagcgcggag cggcggtgac gggaatcatc gacggcaaga tctacgtcgc gggtggcttt
      361 cgcggcgggc gcgcagcgac gctcgtcgac gtttacgatc cggtcgcgga tacgtgggtg
      421 gcgcgcgcgg atctgccggt tgcgcgcgat cacgcatgcg gcggtgtgct ggatggcaag
      481 ctcgtcgtcg caggcggccg cgatggatcg ccgagcgggc ccgtgcctga tgtgtgggcg
      541 tacgatccgg cggcgaatac gtggacgcaa cgcgctccga tgccgacggg ccgtggcggc
      601 atggcgtgcg gggtgatcga cggcgcgttg tatacgaccg gcggcgaagg caatgcgaat
      661 gcggcgagcg gcgtgttcga tcaggtcgaa tcgtaccgcg gcgatcaatg gacgcagcat
      721 gcgccgatgc cgcatccgaa gcatggcgtc ggcggggcgg tgtgggacgg cgcgctctat
      781 ctatgtggtg gcgcgaacgt gcaagcgttt ggcgcggtcg cgacgaccga cattctccgg
      841 ccgtagctgc gatagagtcc gcggcatgcg cgttcactat ctcgctctcg ttctcgcctg
      901 cgccgcatgc tcgaagaaga gcaacgacgc tcccaaagcc actggcagcg ccacgcccaa
      961 gacagcggaa gcaccgaagc cgaagtcgga cgcgccgtgg gacttcgacg cggcggcgct
     1021 gcaggcgaag tttcagggcg cgtgggtcgt caaggaccac gggtatctcg gctcgtcgga
     1081 agcgtgggaa gtgaaaggcg accaggtgaa gatctgggat gccaagcgcg acaaggagac
     1141 gacggcgacg ctgaacgtga tgggcccgtg cgtgtactcg atgaaagagg ccgacggctc
     1201 gtcgacgacc agccactacg tcatcgatgg cgacacgctg catatgggcc ttggcgacgc
     1261 gggggtgaag gtcggcgata aggtgatcgc ctgcatgtcg aacggcgtgt ttgtcctcga
     1321 cggcggcaag tgcacgttct atctcgacca cttcggcaag tggtctcccg aggagggcac
     1381 gtgcagcctc gaaggcgaca cgttcaaggc gtcgaatacg tcgttcaagt acgaaggctc
     1441 gatgcccgcg catggcagcg tcttcgcgag cgaccagctc tggaacaatc cgccgcccgt
     1501 caaggccgcg agctacgacg aggcgaaggc caaggcgaag ggctgagctg acgtatactc
     1561 gtcttcggtg ccaaccgacg acgacgacct gggctccgcc gcgacgatcg tcggcgcgtc
     1621 cacggatgca ggcgccggca cgggcgccgg aaacgacgcg acgcagatga cgcagtcgtc
     1681 gctgccatcg tcgcgcgact cgtcgcccgt gattccgccg agcgtcattg cagatcgcta
     1741 cgagatcctc gggctgcttg gcgtcggcgg catgggccgc gtttatcgtg cacacgaccg
     1801 cgcactcgac gagcaagtgg cgctgaagct gctcaagcgc gagctcgtcg gtgttgccgg
     1861 catgctcgat cgctttcgcc aagaggtgaa gctcgcgcgt cgcgtgacga gcccgcacgt
     1921 cgttcgcacg ttcgacctcg gcgagcacgc gggcgagcac ttcctgacga tggagctcgt
     1981 cgagggccgc tctctcgcgc agctgctcga tgatgggcag ctcgccgtcg acgaaattct
     2041 gcgcatctcg cgggcgatgg cagcgggcat cgcggcggcg cacgcaaccg gcgtcacgca
     2101 tcgcgatttg aagccagaca acgtcctcgt cggcaaagac ggccgcgtcg cgatcaccga
     2161 tttcggcatt gctcgcgccg cagcggtgtc accgcgagag accgtcgatg gcttcgtcgg
     2221 cacgcccgcg tacatggcgc cggagcaagt cgagggcagc gacacgatcg gccccgccag
     2281 cgacgtctac gcgttcggcg cgattctgtt cgagatgctc gcaggtcgcc gcccgttcgt
     2341 cggcagcgat ttgatcgcgg tcgcggtggc gcggcttcgc gaagcaccgc ccgatccgcg
     2401 gcagttccgc cgcatgcccg actcactcgc tgacctcgct ctgcgttgcc tcgagcgcga
     2461 cgccgcacaa cggttcgcca atggcggtga gctcgcgaag gcactcgccg cggtcaccac
     2521 cgatggaccg tcgacgcttg tcaatcagcg accgcaacgc gtgcccgcaa agacgtcgcg
     2581 ctccgtcgcg atcatgccgc tgcgcgcgag cggcgagctc gcggaagttg ccgacggcct
     2641 cagcgaagaa atcgtcgatg cgctctcgat gacacgcgag ctgcgcgtgc ggccactcgt
     2701 gtcgctgcgc aatgccgcgc gcgcggagca agactcgcga gagctgggca agagcctcgg
     2761 cgtcgatgtc gtcgttgacg gatcgatccg caagcgcggc gcgaccgcgg tgcggattgc
     2821 cgctcgcgtg atcggtgtcg aggacggctt tcagctgtgg gcgaaccact tcgatacgag
     2881 cgaggacgat ctgctcgccg caagcgacca agtcgtgcgc gcgatcgcaa atgcgctcac
     2941 cgtcgagctc gaagtgccgc agcgcgcgaa gctcgatcca aaggtcgcgg acgtctatct
     3001 cgagacgaaa gcgaagctgc gaacgaactg gctcgccggc ggtctcgaag atctgttcga
     3061 gcctctcgag gccgcacacc gcgtcgcgcc cgacgacgcg ggcatcatct cgacactctc
     3121 gcttgctcac tcgcggtcgg cgttctacgg cggtcagggc atcctcgcac gcggacgcga
     3181 gctcgcggat cgggcaaccc gactcgcgcc cacctcgggc gaggcctggc tcgcgctcgg
     3241 cgttgcggcc ctgtacggcg gcgatatggc agctgccacg cacgcgttga tcgaggcctg
     3301 cgagcacgcg ccgggtctcg cgctcgcgca ggcgctcctc ggtgcggttt gtctcgaggc
     3361 cggccatctc aacggcgccc tcctgcatct cgaaggcgcg cagtcgctcg atccgttcgg
     3421 cccccagctc gccgatctgc cgcgcgcgta cgtgtacgcc ggtcgcgaag acgaagcgat
     3481 gcgcatgctc gaagcgcaga gcaccgagta tcggctcgtg tcgatcgcgc gcttcaagat
     3541 gtggaagggc gagcgctaca cgatcaacta cacgccatcg ctcccgccga cgctgccacc
     3601 gaacttctcg aagtacgcac gcgtcgccgc caacgttcat cgcaccgcca tgatctccgc
     3661 cgaggatcgt gcggtgatgg atgagatcat tgccgtcgag aatccccgat tgagcgcgac
     3721 gcgagcgcag ttcgttgccg agttctacat cttctgcggc gagctcgatg cagcgatggg
     3781 cgcgatcgaa atggccgtga agaacgggct gcaggatcac ctgtggatgc agaaatgccc
     3841 gctgctcgac agcctgcgtg accggccgcg cttcatcgac ctcgcaacga tcgtcgccgc
     3901 tcgcgcgcgc gtcgtcctcg acgtgatcga ccggctgacg actggcgagt ttcgaaccgc
     3961 tagctagagc acgagcgcgt gcgggttcgt cccgaacggc tggtccggac acttcagtgc
     4021 agcggcgagg tacgcccaca ggccttggat gctcggcgtt ccgctcggca gcgcgacgtc
     4081 cgcattcacg cggcctgttg tgcctgcctt gacgtactta ccgatcaccg tcgccgtcag
     4141 gttcgcctgg tgattcgagc ccggtagcga gcgcgagaag tcgccgacga tcgcggtgac
     4201 gacgttgcgc cccgtctccg ccatcatgcg atccgtgaag accttgagcg cggccatcgt
     4261 gccgtcgccg ttgatcttgt tgcggacctc ggcgccgttg ttatcgccgt gcgaatccca
     4321 accgccttgg ttcgcgatca cgacgtttgc ccccgcgcga atcattagct ctgcgcccat
     4381 gatctgcatc gtcgtgttgc ggacatccgt cgcgaacgag ccgccggtcc cagcggtgag
     4441 gccatacgca gtcgccatgc tcgcgtagtc gagcgcgacg gtcgcttctt tcagctgcga
     4501 tgacgccgcg ggatagcttt ctgcaaacgt gcgcgcggat gtcgggttct tcgcgatcgc
     4561 atccgcggac atcgcttgcg ccgctgcgat gccatccgcc gcgaggccgc gcgctggagc
     4621 gttgccgctc gtgacgccgc cgagcactgc gatcgtcgtc gacaggtcgc gcacttgctg
     4681 gagcgaaacg tcaccttgcg cggtgtgcgt accgacgggc atcaggttgc cgatcgtcac
     4741 gcagcgaacc gccgctgtcc cgccgagcgc cttcgaaagc gagatcaggc ggctcttggt
     4801 gccgtcgaac agcagcgaca tgcgcgccgt cggatgcgac gtgatgccgt gatcgacgcc
     4861 gatcgacgcc gtatggccga gcgtggtcgc cgacagcgtg ccgagcgtcg acttgtcgac
     4921 gaagagcttc gtcgtgccga cctgcttgat gttcgtcgcg gtcacgccga acgcgccggt
     4981 gccctggaac gagtcggcac tgccgaacag cgcgttgtag ccgccgatca tgttgaagat
     5041 gaacagcgcc ggcgcttcac ccgcgacatc tgcgcgagcc tcgcgctcga gccagctcat
     5101 gcccgggatg cggccgacac tgaatgcgcc cgctgcaacc gccgcacgct tgaagagtcc
     5161 acgtcgcgag aaatctttgc tcatggtcat ctcctagtac gcgaggaagc cggacgcggt
     5221 gagcacagcg gcgcaggagt aagcccagcg cacgcgcggt gcatcgctcg cattcgtttg
     5281 gttaacggtg tagttcacgc acgcatcggt ctccgcttgc agcggttcac ggtgccacgc
     5341 gcggcgaagc gtctcgttgc agagcagcgg tgcgttcgtc gcatcgggtg ccgccgcaaa
     5401 cttcgcatcg gtcgcggtcg cgcgcgtgca gccgtcatac gcgagagcaa acgccgcgta
     5461 gatcgtgttc gcgctcgctg ccggctcttg gtaccagcgc gccggcggcc ttccgaacgt
     5521 cgccgcatag gcggccgtgt cgaagcccgt gagcccgagt gcggccgcgt actccgccgc
     5581 gagtgccgcg aacggcttca tgcgaaggcg atcggtgccg gccttcatcg tcgggcgatc
     5641 cgcttcgagc gtcttgccgc cgtagccggt gtacgagcga gtggcctcgc agacttcctg
     5701 cgtgtcgtcg tcgccggtct gatcgtcgcc agacgagcca tcgatctgac cgacgcaacc
     5761 tgcgagcgaa aggattaaga acatgcgctt catcgtcgac tcctactcgc agtaaccggg
     5821 gtcagcagcg atggtccgca aagcagtgcg gatgtcgccg gtctgggtga acgcatcgat
     5881 gcacttgtcg aagagagctg attcgcagct cgcctccggc cgtccataga ggaagacgaa
     5941 tgccatgcgg cacgtgcgga acttgtagtc cgtcgactcg agcagcgccg agacgagctc
     6001 gccgtcgttc gagatcgtct tgccgtcgag gaccgtctgc gggccttcgg tcggcgccga
     6061 gaacgtaatc ggcgtcgtgt cgacgcgctt cgagagaatc ttcgcaacct tgtcgagcgc
     6121 gaagtacgga tcgtagtggc agccgcggca gttgccggac atgcggccgg tcgccgagac
     6181 atcgacacca ggcgcgttcg tcacagcgcc gacatcgaga ccgatgatgt tctgctggat
     6241 gcggaacgca gcagagaggc gatagccgtc ttcttcgttg ccggcgtagc gaaccatcca
     6301 cggccgcgag cggaagtaac cgagcccgtt cgggtcggcg acgatctgcg cggttgcggg
     6361 gtttccattc gccgccgcga cgggctggac gtcgtacttg ccgtcgaaca tctcgtgcca
     6421 cggcttttga ttctcgagca cgtacttcgc catgaagtac gtcgcgtcct gcgcgggcgc
     6481 catcgccggc tcgggattga gctcgctgtt cacgaaccgc gagaactgtt cgacgaacgc
     6541 agggtccgcg agcagcgtat cgatctgcgc ctgcggatct gccgccgcga gcaacgccgc
     6601 attcggcgcc ttgcccgtca acgacagcga gagccgcgtc gcgcagcgaa cctttgccgc
     6661 ctcgtccgcg ggcgtctggc tatgagacgt ccccgagctc gaccacagcg ccacggcggc
     6721 gagcggtacg ccaagaccga acagatatct gactcgcatg gacggctcat agagcaaggg
     6781 tgtggccaac aaccccacct tgggagtcgg gcacttagac ggcgctcgaa gaccggtgtt
     6841 gggtagcccc tacaaacgat tgaaggcagt tgcctgctac ttcgcgtgtc gcgcgctgtc
     6901 tggtatcgcg atgagatgga ctctggtgct ggtcaccggc tgctggacct ctgcgccgaa
     6961 cgcccccgca cggccacccg tcatcgagcc cgagtcgacc tgcatgtcgc tcacggccga
     7021 gacccttcgc aatcgcgatc tgcttccgat gaagcaagcc gagggcgcac cgatcacgaa
     7081 ggagtcaccg ctgcgcgccg tcgcaatgtc gggtccgtat tggaacgtgg cggcagcatg
     7141 cgaggactgc gagcgcggcg acggcagcgg ccgctacctc gagatcgcaa cgggcggcag
     7201 aaagctcgcg atccgcatcg gcaacaagtg gtggtcgacg atgctcgcga tcaacgacac
     7261 gtgcgcgctc cacttcgagc cggtggtggc tcgcgatctg gtcgcgccgg cgccgggcga
     7321 cgagctgttt ctgcaagtca cccaggactg cgccgagccg caggatccat ttcaagagcc
     7381 ggactttcag gagcacatcg tgatgtgcgg agttggccgg tcgcagcgcc cctcctgcgt
     7441 cgaagcagac tttggcgtca caggcattac accggaatgg tcggagcaca cgcgactcga
     7501 tctcgcgtgc gacggcatcg cgacattcac cggttggcac ggcgaagacg gcgaaggatt
     7561 cacgtccgtg cacacgcgcc gccagctcgt gtttccgtag cgcgtgatac gttcatagag
     7621 gcgatgaaac cgggggagct cgtcgaggac cgcttcgagc tcgagcgcgt cgcgggcagt
     7681 ggcgggatgg ggacggtcta ccgcgcgatc gaccgcacga gtggcagtcc ggtcgcgctc
     7741 aaagtcctgc gcagcgcctc gatggatgcg gcgcaacggt tcacgcgcga ggtgcgaatc
     7801 ctctcgcagc tgcgccatcc cggcatcgtt cgatacatcg ccgatgggca gaccgacggc
     7861 ggtgagctct ggctcgcgat ggagtggctc gaaggcgaga gcctaaacgt gcgacttgcg
     7921 cgagatggcc tgacggcggg cgagagcgtc gagctcgtgc ggcgcgtggc cgaggcactg
     7981 ggcgcggcgc acgagaaggg tgtgatccat cgcgacttga agccgtcgaa cttgttcatt
     8041 gccggcggcg atctggagcg ggtgaaggtg ctcgatttcg gcgtcgcgcg catgacggac
     8101 aattcggcca cgcgcacggg cgtcatgatc ggaacgccgg gctacatggc accggagcag
     8161 gcaaagggcg agcgcaacgt cggatcgcga tcggacgtgt tcgcgctcgg gtgcctcttg
     8221 tacgagtgcc tcaccggcaa gccgccattt ctcggcgaca acgtgatggc ggtactcgcg
     8281 aaggtgctgc tcgaggaggc gccgcgcgtc ggtgtcgtga ggccggatct gccggaggcg
     8341 ctcgacgatc tcgtggcgcg cacgctggcg aagaatccag atcatcggcc gcgcgatggc
     8401 gcggagctcg cgatcgagct ggcgacgctc ggcaccatcc atgcggcgga gcgcccggcg
     8461 aaccagccca gcgctgcgct cacggcgacg gagcggcgac tgctgtgcgt cgtcctgatc
     8521 gatgtcggat cgcgcgagcc cgtcgatagc gacgcgacaa tccagagcct gccgtcgatg
     8581 atggacaaca cgctgccgtc ggagtcgagc cagccgcgcg acacgctcgc cgagtcgatg
     8641 cgccccgagc tgaagagctt gcgcgagacc gccgagtcac atggcgcacg cttgcagagg
     8701 ctcgtcgacg gctccctcgt cgtgacgctg ctcggcaccg gcggcaccgc ggatcaagcg
     8761 acgcaagccg cacgctgcgc acttgcactc aagcagcagc tgccgggaat cccgatggcg
     8821 ctcgcgaccg gtcgcggcgt gatggccggc cggtggccga tcggggaagc gatcgatcgc
     8881 gcatcgggct tgctcctcgc ggcacgcagc gcatcacccc atctcgtgcg cgtcgacgaa
     8941 gtaacggccg gtctcctcga cacgcgcttc gaagtcggcg gtgacgaagc cggcctgtat
     9001 ctcctcggcg agcgcgacct cgtcgaagcg cggcgcacgc tgctcggcaa gcccacgccg
     9061 tgcgtcggac gcgatcgcga gctcggcgtg ctcgcaggtc tcttcgacga gtgcgtctcc
     9121 gagccgatgg cgcgtgcggt gatcatcacg ggcagtgccg gtgtcggtaa gtcgcgtgtg
     9181 cgctacgagc tgctccgccg catcaagcag cgcgaggagc ctgtcgagac ttggatcggc
     9241 cgcggcgatc cgcttcgaac cggctcaccg ttcggcatga tcgcacccgc gctccgccgc
     9301 gcatcgggca tcgccgatgg cgaaccgatc gaagtgcggc ggcaaaagct gcgcgcgcgc
     9361 gtgatgcgtc acgcgacgac cgaagtgaac cgcacgacct tcttccttgg cgagctgatg
     9421 ggcgtcacgt ttcccgacga ggaatcggtc gagctgcact ccgcgcgaca agacatgcag
     9481 ctgatggccg atcaaacgcg ccgcgcgttc gagcacttcc tcgcgatgga ggcatcggcg
     9541 cagccggtcg tgatcgtcct cgaggatctg cactggggtg acctcccgac cgtgaagctg
     9601 atcgattcgg cgttgcgcgc cctgcccgac ttaccgtggt tcgtcgtcgc ggtcgcacgc
     9661 ccagagctgc acgagctgtt ccctcgcatg tgggcggagc gtggcgtgca agagctgagg
     9721 ctcgacgagc tgacgaagcg cggcagcgag aagctcgtgc gctccgtgct cggcgacaaa
     9781 gccaacgacg cgctcgtcgc acgcatcgtc gagcaagcag gcggcaacgc gttctatctc
     9841 gaggagctca tccgcgcagt cgccgaaggc aaaggcgaga cgaagattcc agagacggtg
     9901 ctcgccgtcg tgcaaggtcg tctcgaacgg ctcgaggtcg atgcgcgccg cgtgctgcga
     9961 gcg
//
LOCUS       DATLBZ010000424         8442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2758972, whole genome shotgun sequence.
ACCESSION   DATLBZ010000424 DATLBZ010000000
VERSION     DATLBZ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2758972"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VMZ53_21515"
     CDS             complement(<1..200)
                     /locus_tag="VMZ53_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31110.1"
                     /translation="MQLGGKRIFHYTLPANAQLAKLAVREADGTTRVLVDPEALSTPE
                     VHVSLHAYSATHDGTRVAYVIS"
     gene            171..1826
                     /locus_tag="VMZ53_21520"
     CDS             171..1826
                     /locus_tag="VMZ53_21520"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HUS31111.1"
                     /translation="MEDSLAAELHVMDVKTGKDLPDVIDRIWGEGSASWLPDDKSFFY
                     TQLAEPKPGVDPMTGQVAKLHVMGTPVDNDPIVLGRADAATWKLAPEEWPGLWQPTGS
                     TWVVVTAGGAHSEMRVGVAKTSELDKTGASKTPWRVVAEYADGIEQAWPHGDRLYMMQ
                     YKDAPNRKLVSVPLAKPVLANARVELAEDPAMKIESVIPAKDGMYVMRQMNGLMRLDR
                     LAWKAGAKPEAIALPYEGWIPDAYGDMATEGLFFQVESWLRIGAYYAWNPKKKQLAPA
                     GLASTSNADVSQLVAEETEATSFDGTKVPLTIIHRKSDVASTPHPTLLYAYGGYGAVE
                     RPGFSSSRIAWIERGGIYAIAHVRGGGEKGRKWQDDGSREKKMNGVRDFIACAEFLVA
                     KGYTSTKKLAIHGISMGGVLVGRALTERPDLFGVVQLAVGIVNPLRILEAENGANQKL
                     ELGDPTTDGGYKSIAEMDPYTHVKPSTAYPAVVFTIGLNDHRVAPWMTAKMAARLQAA
                     TTSKRPILVRVDRDAGHGVGSTRDQGFAEKADIWGFFLDQMGE"
     gene            1945..2346
                     /locus_tag="VMZ53_21525"
     CDS             1945..2346
                     /locus_tag="VMZ53_21525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31112.1"
                     /translation="MRCRLVVVAVLLDLTAPAVACPPLDTCLVQLPSTTRLPHVSPAH
                     VRVPASQDAAVSEVGQPPNLTEIIRELTPPPRPAPGEVEMPWIWMALRAQVYSRMPTY
                     EQSHDFKIVLSPVVVSSPSDTVPGVGIAGDF"
     gene            2385..3239
                     /locus_tag="VMZ53_21530"
     CDS             2385..3239
                     /locus_tag="VMZ53_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31113.1"
                     /translation="MGRVLVLAFVLLACGGSPPPPPSPPAPAPSQLVQPAVANEMPAT
                     LAKHMTSHGWGPMHLQWHTERRWDQLPPAAVDYAKRQGWRRAERQEGSVGNGVEFLAM
                     HRAMIELLADADPSEKAVLAGWTTPPTDPTDPADPLPNGATTAFDPDMAKALDRLAHH
                     LDTFASEDELGLFIETWFRPTTDAPKTRAVDRTAGVHNYLHQRFQDPTSPIDLGNPST
                     NLMNPRFWRLHGWIDHVWAAYRAQKGLRDTDAAYQQLIGDAKAHMSMRAKAPLEAPPA
                     ELLDVVRP"
     gene            3305..3907
                     /locus_tag="VMZ53_21535"
     CDS             3305..3907
                     /locus_tag="VMZ53_21535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS31114.1"
                     /translation="MGRASTAHRSTSAGRAELDDLTLRRAQQGERTALDQLVERYHPV
                     VHALVWRMACAQGDSHVADLVQDSLIRVLQNVARFDPAGPARLSTWILTITTRVVLND
                     RRRPPAREMKHEPFAYVDPATAASERELGAAIAKAVVELPEPQRIAFVLREYHDLDYA
                     DIAKALEIDTGTVKSRLSRARAAVRDTLLRTHPDLFRGAR"
     gene            3904..4782
                     /locus_tag="VMZ53_21540"
     CDS             3904..4782
                     /locus_tag="VMZ53_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31115.1"
                     /translation="MTHDDTDAGEDLLANIDLHAWRVPPPVAVHRASHLVGALSPAAP
                     AKRPRLKWLVAGLVLLNAAIAAVLVIVLARPAPERQTVVVQPAGGGTVDAQVRDLLRR
                     LDVEQKELERKLAEIEELRSLVIELSEKVRQYEERDRREKTVTRRQPPVTRQPIDPYD
                     PYDARNTSPPSGGACDEVSCVLGNYQGGCCEKFKKSSPPPPPKNDLPESLTRETISTA
                     MASVKSQVTLCGDVYKVHGKVKVNVKVSPNGAVTNVEIAATPDPQLGACVAGVIQHAV
                     FAKSQVGGSFSYPFVF"
     gene            4810..5769
                     /locus_tag="VMZ53_21545"
     CDS             4810..5769
                     /locus_tag="VMZ53_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31116.1"
                     /translation="MRAGASTVVVAVFLVACGDGVDDWDGVRNPLLGFDDAAIKDAYL
                     VHDGDTWHLGYSRATADPFRFQLGFASSPNLRDFTIGDTLDQPDTGGLASPTVVRAPD
                     GRWLMTYNSHTRDIGDSLNKLYYRTSDDLATWSAATRIHIDGVDDPSERLIDAAVAFT
                     DTGAYLFFKRDQIANVAYAASGSIDGPWTLLGELSQRNLENLQPILIDGTWHLLGTTI
                     PLVHDAVLHRLDGDPTAPASWRNWTVVRTLTVEEQSWNTGPLLDHERSNAAFLVDDRP
                     ADGYFYLLYAGSTDLTSFEGRGHAKLGLARSRDLQTWDAAPER"
     gene            5781..6917
                     /locus_tag="VMZ53_21550"
     CDS             5781..6917
                     /locus_tag="VMZ53_21550"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HUS31117.1"
                     /translation="MFSRALASLALALPTLLLAACLMTPVMRFGEGKTAKQAQRDTMT
                     EIGPSRLATGETWKGEVTTRTVRVYADGPYRTQNIHWQDTFNEALELTNIVLTPLFGV
                     RLVADYHVWDRDVPGATLADSLDALRAHDPGDDNIFAVIGLTSSLPLVSATFDQLGYA
                     SVGGRHIMLRGYADLEERKAYANAFPDLTPEERELALVHLRHHKSAVILLHELGHNLG
                     MDHEVAENTIMNASYSSHATAFSPTARSTMLASVDKRLNRASSTTPPSSTPTPTPTPT
                     PVLASAPPPPHATTPSPTTDTPTMPHDPILIRVTKKAATMVNGKTLDTDALDTLLKAT
                     FAEAPKTRIIISQDRNLPPGVMGDLLDRLKAAGFAKVEFAWSGH"
     gene            7033..7108
                     /locus_tag="VMZ53_21555"
     tRNA            7033..7108
                     /locus_tag="VMZ53_21555"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7066..7068,aa:Ala,seq:cgc)
     gene            complement(7176..8183)
                     /locus_tag="VMZ53_21560"
     CDS             complement(7176..8183)
                     /locus_tag="VMZ53_21560"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUS31118.1"
                     /translation="MANTRAYVWLCTLAALGCGGGTGLPDAATRSDTTTSDVTINDSS
                     PDAGGAACGPTGTSPCSESTAVPGGTFYRSYDLAADGLFSNMSYPATVSGFRLDKYEV
                     TVGRFRQFALAGMGTQSSPPAAGGGSRSLNGMSDQGGWDPAWNTNLPINTAALTAAVK
                     CGGGSTWTDSAGANETLPIDCISWFEAMAFCIWDGGFLPTDAEWNYAAAGGDEQRAYP
                     WSNPNSSVTIDCTHANYAGGCAGALNRVGSESPAGDGKWGHSDLAGNIMEWTLDWYTS
                     PYPTPCDNCAQLTQASDNRVLRGGAYLESAMLVRAGVRRGGFTPFDRFPYIGVRCARA
                     P"
BASE COUNT         1534 a         2886 c         2609 g         1413 t
ORIGIN      
        1 ttcgagatga cgtacgcaac gcgcgtccca tcgtgcgtcg cgctgtacgc gtgcaacgag
       61 acatgcacct cgggcgtcga cagcgcctcc gggtcaacga gcacgcgcgt cgtgccatcg
      121 gcctcgcgga ccgcgagctt cgcgagctgc gcgttcgcag ggagcgtgta gtggaagatt
      181 cgcttgccgc cgagctgcac gtcatggacg tgaagaccgg caaggatctg ccggacgtca
      241 tcgatcggat ttggggcgaa ggctcggcga gctggttgcc cgacgacaaa tcattcttct
      301 acacgcagct cgcggagcca aagcccggcg ttgatccgat gaccgggcag gttgcgaagc
      361 tccatgtgat gggcacgccc gtcgacaacg acccgatcgt cctcggacgc gcggacgcgg
      421 cgacgtggaa gctcgcgcct gaagagtggc ccggactgtg gcagccgaca ggaagcacgt
      481 gggtcgtcgt gacggcgggc ggtgcccaca gcgagatgcg tgttggtgtc gcgaagacga
      541 gcgagctcga caagacgggc gcgagcaaga cgccatggcg cgtcgttgcc gagtatgcgg
      601 atggcatcga gcaggcgtgg ccgcacggcg atcggctgta catgatgcag tacaaagacg
      661 cgcccaatcg caagctcgtg tcggtgccgc tcgcgaagcc cgtgctcgca aatgcgcgcg
      721 tggagctcgc cgaggatccg gcgatgaaga tcgagtcggt gattcccgcg aaggacggca
      781 tgtacgtcat gcggcagatg aacggcctca tgcggctcga tcggcttgcc tggaaagccg
      841 gcgcgaagcc cgaggcgatc gccctgccct acgagggctg gattcccgat gcgtacggcg
      901 acatggcgac cgaaggcctg ttctttcaag tcgagagctg gctgcgcatc ggcgcgtact
      961 acgcgtggaa tccgaagaag aagcagctcg cgcccgcggg tcttgcctcg acgagcaacg
     1021 ccgatgtttc gcagctcgtc gctgaagaga cggaagccac gagcttcgat ggaacgaagg
     1081 ttccgctcac gatcattcat cgcaagagtg atgtcgcgag cactccgcat ccaacgctgc
     1141 tgtacgcgta cggcgggtat ggtgccgtcg agcggccagg cttctcgtcg tcgcgcattg
     1201 cgtggatcga gcgcggcggc atctatgcga tcgcgcacgt gcgtggtggc ggggagaaag
     1261 gtcgcaagtg gcaggacgac ggctcgcgcg agaagaagat gaacggcgtg cgtgacttca
     1321 ttgcgtgtgc cgaattcctc gtcgcgaagg gctacacctc gacgaagaag ctggcgattc
     1381 acggcatcag catgggaggc gtgctcgtcg gtcgcgcgct gaccgagcgg ccggatctgt
     1441 tcggcgtcgt gcagctcgcg gtcggcatcg tcaatccgct gcgcattctc gaagccgaga
     1501 acggtgcgaa ccaaaagctc gagctcggcg atccgacaac ggatggcggc tacaagtcga
     1561 tcgccgagat ggatccgtac acgcacgtga agccgagcac ggcgtatccg gcggtcgtgt
     1621 tcacgatcgg gctcaacgac catcgcgtcg cgccgtggat gaccgcgaag atggctgcgc
     1681 gactccaagc agcgacgacg agcaagcgac ccatcctcgt gcgcgtcgat cgcgatgcag
     1741 gccatggcgt cggctcgacg cgcgatcaag gcttcgcgga gaaggccgac atctggggct
     1801 tcttcctcga ccagatgggt gagtgacgcg cggccgcgca cgtcaggttc gcgacactgc
     1861 gcactcttgc tcgcaactcc gcacgcgtcc gcgcgaactt cgccatcgaa tacctcggta
     1921 cgccacttgc gttgatctcg catcgtgcgt tgccggctcg tcgtcgttgc agtccttctg
     1981 gatttgaccg ctcccgcggt cgcgtgccca cccctcgata cgtgcctggt ccagctcccc
     2041 tcgacgacgc gcctgccgca cgtgtcaccg gcgcacgtcc gtgttccggc gtcgcaagat
     2101 gccgcggtct cggaggtcgg ccagccgccg aacctcacgg agatcatccg cgagctcacg
     2161 ccgccgccgc gccccgctcc gggcgaggtc gagatgccgt ggatctggat ggcgcttcgc
     2221 gcgcaggtct actcgcgcat gccgacgtac gaacagagcc acgacttcaa gatcgtgctc
     2281 tcgccggtcg tggtgtcgag cccgtcggat acggtgcccg gcgtcggcat cgctggcgac
     2341 ttctagctcg gtaggctgtt cttgggcgct atgatgcgcg gcctatgggt cgcgtgctcg
     2401 tcctagcgtt cgtgctgctc gcgtgcggag gttcgccgcc tcccccgcca tcgcctcccg
     2461 cgcccgctcc gtcgcagctg gtgcaaccag cggtcgcgaa cgagatgccg gcgacgctcg
     2521 cgaagcacat gacttcacac ggttggggac cgatgcacct gcagtggcat accgaacggc
     2581 gttgggatca gctgccgccc gctgcggtcg actatgcgaa gcggcaaggc tggaggcgcg
     2641 ccgagcgcca ggaaggcagc gtcggcaacg gcgtcgaatt tctcgcgatg catcgcgcga
     2701 tgatcgagct gctcgccgac gccgacccga gcgagaaggc cgtgctcgcg ggttggacga
     2761 ccccgccgac agatccgaca gatccagccg atcctctgcc caatggcgca acgactgcgt
     2821 tcgatccgga catggcgaag gctctcgatc ggctcgcgca tcatctagat accttcgcgt
     2881 ccgaagacga gctcggcctc ttcatcgaga catggttccg gccgacgacc gatgcaccga
     2941 agacgcgtgc cgtcgaccgc acggccggcg tgcacaacta tctgcaccag cgcttccaag
     3001 atccgacgag cccgatcgat ctcggaaatc cgtcgacgaa cctgatgaac ccgcgattct
     3061 ggcgcctgca cggctggatc gatcacgtgt gggccgcgta ccgcgcgcag aaaggcctgc
     3121 gcgataccga cgctgcgtat caacagctca tcggcgacgc aaaggcgcat atgtcgatgc
     3181 gcgcaaaggc gccgctcgaa gcaccgcccg cggagctgct cgacgtcgtg cggccgtagc
     3241 cagcaatctc gaagggttac gttttcgtgg aaccaatcgc cgcgctcgcg tgtgcaacgt
     3301 aacgatggga cgagcctcga ccgcacatcg ctccacatcg gccggtcgcg ccgagctgga
     3361 cgatctgacg ctgcgacgag cgcagcaagg cgaacgcacg gcactcgatc agctcgtcga
     3421 acgctatcac ccggtcgtgc acgcgctcgt gtggcgcatg gcatgcgcgc aaggtgacag
     3481 ccacgtcgct gacctcgtgc aggactcgct cattcgcgtg cttcagaacg tcgcgcgctt
     3541 cgacccggcg ggaccagcac gcctgtcgac atggatcctg acgatcacaa cgcgcgttgt
     3601 cctcaacgat cgtcgacgcc ctcccgcgcg cgagatgaag cacgagccgt tcgcgtatgt
     3661 cgatcccgcc accgctgcgt ctgaacgcga gctcggtgcg gcgatcgcga aggcggttgt
     3721 cgagcttccc gagccgcagc ggatcgcgtt cgtgctgcgc gagtatcacg acctggatta
     3781 cgccgacatc gcgaaggcgc tcgagatcga caccggcacg gtgaagtctc gcctctcacg
     3841 cgcacgcgcg gccgttcgcg acaccctcct gcgcacgcat cccgatctct tccgaggtgc
     3901 acgatgactc acgacgatac cgacgcaggc gaagacctcc tcgcgaacat cgatctgcat
     3961 gcgtggcgtg taccgccgcc cgtggccgtc catcgcgcgt cgcacctcgt aggtgctctc
     4021 tcgccggcgg cgcccgctaa gcgtccgcgc ttgaagtggc tcgtcgcggg gctcgtgctc
     4081 ctcaacgcgg cgatcgcggc cgtcctcgtg atcgtcctcg cgcgtccagc accggagcgc
     4141 cagaccgtcg tcgtgcagcc ggccggcggc gggaccgtcg atgcgcaggt tcgtgacttg
     4201 ctgcgacggc tcgacgtcga gcagaaagag ctcgagcgca agctcgccga gatcgaggag
     4261 ctgcgttcgc tcgtgatcga gctgtcggag aaagtccgcc agtacgagga gcgagatcgt
     4321 cgcgagaaga ccgtgacgcg ccgacaaccg ccggtcactc gacaaccgat cgacccgtac
     4381 gatccgtacg acgcccgcaa cacctcgcct ccgtcgggcg gcgcgtgcga cgaagtctcc
     4441 tgcgtgctcg gcaactatca gggcggttgc tgcgagaagt tcaagaagtc ttcgccgccg
     4501 ccgccgccga agaacgacct tcccgagagt ctgacgcgcg agacgatttc caccgcgatg
     4561 gcgtcggtga aatcgcaggt aacgctgtgt ggcgatgttt acaaggtgca cggcaaggtg
     4621 aaggtcaacg tgaaggtcag cccgaacggt gccgtgacga atgtcgagat cgcggcgacg
     4681 cccgatccgc agctcggcgc atgcgtcgcg ggcgtgatcc aacacgccgt cttcgccaag
     4741 tcgcaggtgg gtggctcgtt ctcgtatccg ttcgtgttct gagcgtcgac ggtcgggtac
     4801 gctggtgcgg tgcgagccgg cgcaagtacc gtcgtcgtgg ccgtattcct cgtcgcatgt
     4861 ggcgatgggg tcgacgactg ggacggcgtt cgcaatccgc tgctcggctt cgacgacgcc
     4921 gcgatcaaag acgcgtacct cgtgcacgac ggcgacacgt ggcacctcgg ctacagccgc
     4981 gcgactgcag accccttccg cttccagctc ggctttgcat cgagcccgaa cctgcgcgac
     5041 ttcacgatcg gcgacacgct cgatcaaccg gacacaggtg gcctcgcgtc gcccaccgtc
     5101 gtgcgtgccc ccgacggccg atggctgatg acgtacaact cgcacacccg cgacatcggc
     5161 gactcgctca acaagctcta ctaccgaacg tcggacgatc tcgcgacatg gtcggccgcg
     5221 acgcgcatcc acatcgatgg cgtcgacgat ccgagcgagc gtttgatcga cgccgccgtc
     5281 gcattcaccg acaccggcgc ctacctcttc ttcaagcgcg atcagatcgc gaacgtcgcg
     5341 tacgccgcga gcggctcgat cgatggacca tggacgctgc tcggcgagct ctcgcaacgc
     5401 aacctcgaaa acctccagcc gatcctcatc gatggtacgt ggcacctcct cggtacgacg
     5461 atcccgctcg tccacgacgc cgtcctccat cgtctcgatg gcgacccgac cgccccagcg
     5521 tcctggcgca actggaccgt cgttcgcacc ctgaccgtgg aggagcaatc ctggaacacc
     5581 ggtcctctcc tcgatcacga acgctcgaac gcggcgtttc tcgtcgacga ccgtcccgcc
     5641 gacggctact tctacctcct ctacgcaggc tccacggacc tcacatcgtt cgaaggtcgc
     5701 ggccacgcga agctcggcct cgcacgcagt cgcgatttgc aaacatggga cgccgcaccc
     5761 gagcgatgat gatgcggacg atgttttcgc gcgcgctcgc ctcgctcgcc ctagcccttc
     5821 cgacgctgct actcgcggcg tgcctcatga cgccggtcat gcgcttcggc gaaggcaaga
     5881 cagccaaaca agcgcagcgc gacacgatga ccgaaatcgg tccatctcgc ctcgcaaccg
     5941 gcgaaacgtg gaaaggcgaa gtcacgactc gcaccgtccg cgtctacgcc gacggcccgt
     6001 accgcacgca aaacatccac tggcaggaca cgttcaacga agcactcgag ctcacgaaca
     6061 ttgtcctcac tcccctgttc ggcgtgcggc tcgtcgccga ctaccacgtg tgggaccgcg
     6121 acgtccccgg cgcaacgctg gccgacagcc tcgacgccct gcgcgcgcac gatccaggcg
     6181 acgacaacat cttcgccgtc atcggcctca cctcctcact cccactcgtc tcggcaacgt
     6241 tcgaccagct cggctacgcg agcgtcggtg gtcgccacat catgctgcgg ggctacgccg
     6301 atctcgaaga acgcaaagcc tacgcaaacg ctttccccga tctcacgccc gaagaacgcg
     6361 agctcgcgct cgtccacctc cgccaccaca agtccgccgt catcctgctc cacgagctcg
     6421 gccacaacct cggcatggat cacgaggtcg cggaaaacac gatcatgaac gcgtcgtact
     6481 cgagtcacgc gaccgccttc agcccgaccg cgcgcagcac gatgctcgcg tccgtcgaca
     6541 aacgcctgaa ccgcgcatca tcgacaacac cgccatcgtc gactccaacg ccgacgccga
     6601 ccccaacgcc ggtactcgca agtgcgcctc cgccacccca tgcaacgact ccgtcgccga
     6661 cgaccgacac acccacgatg cctcacgatc cgatcttgat ccgcgtgacg aagaaggcgg
     6721 cgacgatggt gaacggcaag acgctcgaca ccgacgccct cgacaccctt ctcaaagcga
     6781 cgttcgccga agccccaaag acccgcatca tcatcagcca ggaccgcaac ctgccgcccg
     6841 gcgtgatggg cgacctgctc gaccgcctca aggccgccgg cttcgccaaa gtggagttcg
     6901 cctggtcggg tcactgagcg cggctgccac gctcgccgcg tcgagctccg gccgctaacc
     6961 ccgcgctccc gatgctgatc caggccgtcg cttgacaggt caattcgccg ctgataccca
     7021 tacgccactc ctggggtcgt agctcagctg ggagagcgct agaatcgcac tctagaggtc
     7081 tggggttcga tcccccacgg ctccaccagg aaaacgaaat cggtccgcag caatgcgggc
     7141 cgtttttgtt ttcggtcagc tactcgtcac gatcactacg gtgccctggc gcaccgcacg
     7201 ccaatgtacg ggaaccggtc gaatggagtg aagccgccgc ggcgtactcc ggcgcggacg
     7261 agcatcgcac tctccagata cgcacctccg cgaaggactc ggttgtcgga ggcctgcgtc
     7321 agttgcgcgc agttgtcgca cggcgtggga tacggcgatg tgtaccaatc aagcgtccac
     7381 tccatgatgt tgccggcgag atccgagtgc ccccacttac cgtcgcccgc cggtgattcg
     7441 ctacctactc ggttcagcgc acctgcacag ccgcccgcgt agttcgcatg tgtgcagtcg
     7501 atcgtgaccg aagagtttgg attcgaccag ggatacgctc gctgctcatc gccacccgcg
     7561 gcggcatagt tccactctgc gtctgtcggg agaaacccac catcccaaat gcagaacgcc
     7621 attgcttcga accacgatat gcagtcaatc ggcagcgttt cgtttgcgcc tgccgagtcg
     7681 gtccatgtgg agcctccgcc acacttgact gcggccgtga gcgcagccgt gttgatcggg
     7741 aggttcgtgt tccacgcggg atcccatcct ccttgatctg acatgccgtt gagagatctg
     7801 cttccgccac ccgccgcagg cggactgctc tgcgttccca ttcccgctag ggcgaactgc
     7861 cgaaaccgac cgaccgtcac ttcgtacttg tcgagtcgga agccgctgac cgtagccgga
     7921 tagctcatgt tggaaaacag tccgtcagct gcgagatcgt aactacgata gaacgtcccg
     7981 cctggaacgg cggtgctctc ggaacacggc gaggttcccg tcgggccgca ggccgccccg
     8041 ccggcatctg gcgaagagtc gttgatcgtc acgtcactcg tggtggtgtc actgcgcgtg
     8101 gcggcgtccg gcaatccagt gccgccccca caacccagcg ccgccagcgt gcagagccat
     8161 acgtatgcac gcgtgtttgc catatgactc ggacgctacc accatgactt cttctcgaag
     8221 cgcagttcac gcggatgtca cgcgagaaag aaaaaagcgg cgcggtacgc tcgcaacaga
     8281 aaagacgttc acgttcggac gatcacgctg cgcgctgcgt gatgcggacc gtcttggcgt
     8341 ccatatccgc ggccacgtag cgcgcggaga caagcgcgag caacccggca ccgagcgtcg
     8401 ctgccgtcgg gatcagcatg gcgttcttga gaccccacgc gg
//
LOCUS       DATLBZ010000425        11959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6971577, whole genome shotgun sequence.
ACCESSION   DATLBZ010000425 DATLBZ010000000
VERSION     DATLBZ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6971577"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VMZ53_21565"
     CDS             complement(<1..237)
                     /locus_tag="VMZ53_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31119.1"
                     /translation="MARVDKRNDQGLAYAVAGGGTIGAAWGGDYDGAGVAFGMFAGPV
                     AYEHEGCRPLPTYSVMIGVRSLGGLFEYFVLPKAS"
     gene            complement(545..1090)
                     /locus_tag="VMZ53_21570"
     CDS             complement(545..1090)
                     /locus_tag="VMZ53_21570"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HUS31120.1"
                     /translation="MGIVRAMTWAAAGFGAAHVLESAWHRWIAHGKNADPTRAQHLEH
                     HRVAAQPVDVWSEMRDNAGRYAKTLVGVNLALAPFIGLSRTLPLSVGLTAGLVAVNCY
                     HARMHERAPRTRYEKWMWRFHWHHHAADARVNFGLTNPLLDFALGTAFVPDTVELHPK
                     LVPAWLRNAGGAVAGIRVATD"
     gene            complement(1156..2721)
                     /locus_tag="VMZ53_21575"
     CDS             complement(1156..2721)
                     /locus_tag="VMZ53_21575"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HUS31121.1"
                     /translation="MGKLLCVAWLAALVGCSDDAEPLQISTDTGVVKGLVQGESRMWR
                     GVPYAAPPVGELRWKKPKPAAAWDDVRETFELGAACPQVFSAFGAASGEEDCLYLNIW
                     ASRDKHDAPVLVWFHGGAFAFGSGGETYYNGQHLAETQGVVIVAVNYRLGAFGFLAHA
                     GLAAEDAGYPTSGNYGLEDQLAALQWVQRNIKAFGGDPTHVTVFGESAGGISTCWQYT
                     SSKTAGLFHAAIAESGLCSNNLFFPTRAEAEATGARVATSLGCTGNDAAVATCMRGKT
                     VAQLLTAVQGPKISDQQPGGPFLQPDGLLLAPNVDGLVVEKNSRDAFASGSFMPRPLL
                     LGSNRDEGTLFQSSLVAKETTSESDMRNALATRFTSEQVDAILAHYPPASFPSIDRAI
                     AEITGDGFFVCPTRRAARGAAAAGASVYRYSFEREVENPVLPGLGVFHASEIPFVFGE
                     DSFPFGVVGAGGQPVADKMQTAWTTFAATGNPGADWPKFETASDQLLVLDVTSNVRTG
                     HKAALCDFWDTLP"
     gene            complement(2750..3229)
                     /locus_tag="VMZ53_21580"
     CDS             complement(2750..3229)
                     /locus_tag="VMZ53_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31122.1"
                     /translation="MARAPDIVEMSFVFRDEPGRVLAALLGVGAGAPHVFELPEAAEH
                     AALATRPPRTPVGYSAPNLTTRISTKVRATATWDTRADTLRLEGYFADRATGLPRAPG
                     PRFAIASPSFVMPPPGWEPLFRRLSAARAPLDATFATVSLHHDNGKSIVVIAFDPPR"
     gene            complement(3243..4136)
                     /locus_tag="VMZ53_21585"
     CDS             complement(3243..4136)
                     /locus_tag="VMZ53_21585"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUS31123.1"
                     /translation="MSFLRKMFSGRVSGEDSRRFLVEAMLGAMEADGDISEEEMAAFE
                     GNLANHKMFEGLSADELSRLTDLAADALRVAGGGKARLPAIAEGLPSRGQRLTAYGLA
                     AEICASDKNLAETEIEFLDAMQKAFVLDDSETKDVFEAARKHSGLLTLEEKSEKVRFL
                     LPMFVRCMALMASADEEIHHEERLAMRSILRAIPDMAVLTHAEIDEAIDVALERVAGK
                     DLKKELESVASEVTAGNDRYWVTCYTMIVSLADGVSDWREVEFLANMRATFGLTDTQM
                     DAAMKTAALFPAVKLGGDAPE"
     gene            complement(4154..5386)
                     /locus_tag="VMZ53_21590"
     CDS             complement(4154..5386)
                     /locus_tag="VMZ53_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS31124.1"
                     /translation="MLIDFLFELRQRKIPVSTHEWMALMEAMALGLHESSLDGFYHLC
                     RTICVKDIAFYDQYDEAFLAYFKDIHVDSIKLTDQLQQWLMNPQALEGLTEEQRKALS
                     ELDFEKLRAEFEKRLKEQKERHDKGNRWVGTGGTSPFGTGGMNPTGMRVGGGGGRSAM
                     AVADQRLFKEYRRDVVLDVRQIDMALRGLRRLGREGAQEELDLDDTIEKTGKNAGELE
                     IVFRPPRRNRVKVVLLMDVGGSMDPHAELASRLFTAASRAGRFAKFRSYYFHNCVYNS
                     VYEDAQFRKGVPVADLLANSDRDEKLVMLGDALMHPAELLDGGMMSYGSSYLYSQTRA
                     PGIEWLRRLAHHFRSSAWLNPEPERFWSGTTIEVIASVFPMFAMTLDGLNHAVRYLVR
                     GGEKPRIGDPGKWIKQMG"
     gene            complement(5387..6217)
                     /locus_tag="VMZ53_21595"
     CDS             complement(5387..6217)
                     /locus_tag="VMZ53_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUS31125.1"
                     /translation="MSDDDRQFDQFKGTATYIASEELRHAVNVAVALRRPLLVRGEPG
                     TGKTLLAENLAASLGLPLIRWHVKSTTKAKDGLYVYDTVARLHDSRFGGDVRDIAKYI
                     KLGPLGEALKSPSKVVLLIDEIDKADIEFPNDLLLELDAMRFRIDETGEEVVAKERPV
                     IVITSNNEKELPDAFLRRCVFHFIQFPDRELMTEIVRVHHPDITDRVLDNALELFFGL
                     RATPRLRKKPSTSELIDWIMALKKGGVDLAKVGHGIPFLGTLLKTESDVELVQKRAKA
                     "
     gene            complement(6238..6498)
                     /locus_tag="VMZ53_21600"
     CDS             complement(6238..6498)
                     /locus_tag="VMZ53_21600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31126.1"
                     /translation="MSKALMRQSSGGGSGVVPAVCSALIPGVGQLVNGEGSKALGVFA
                     VSVACGIASAIPLVGGIFAVAGAVTWLYGVGDAYVTGKRGKR"
     gene            complement(6521..8197)
                     /locus_tag="VMZ53_21605"
     CDS             complement(6521..8197)
                     /locus_tag="VMZ53_21605"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HUS31127.1"
                     /translation="MKLGGLTLTVVSCISGTAAAGGLILPGGGAVSTSRAGASVAATE
                     DAESIGLNPAGIAKTTGTKITIGAAAISYFMTFQRNGTYDDNPNDAESYEGQRYPIVT
                     QDAAPPLGIGRYQPVPVIGIVSDLGGKVPNLSVGFGLYAPNAYPFRRMNNVGGQNYYV
                     ESGGAYEYPAFGAPPPPSRYDIIAQEGAVILPSIVAAYRVIPDLDIGVRFSAGFADVK
                     STIAVWGSPANIVEWVKADGLFTLEAKDSFVMALGAGAAYRVGKNIELGLNFTPQTNV
                     TAKGDAHAVNGPGVSLNGEPFHVRPSAATRCAAGGTDEAQKGCVQFALPMTATVGGRY
                     KLTDANDKEKGDVELDVTWEHWGKTCDYVADPGCHSPSDYRVVVDAQVATESAPDNGI
                     DLKDNIVAHGFRDTYGVRLGGSYHFAAGANEVIARGGVAYDTAAAKPGWERADIDGSA
                     RTTLTAGASYKMSRVQIDAGFGAVLEGTRTDSRTCNPIADPSKQSSPPQNVPYSGCGA
                     GDIQQAIDDRQGPDPINPVNTPQSQLENPVNQGTFKSHYLLFMLGVSTWF"
     gene            complement(8310..10910)
                     /locus_tag="VMZ53_21610"
     CDS             complement(8310..10910)
                     /locus_tag="VMZ53_21610"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUS31128.1"
                     /translation="MADESSGGPFDDGLARLLIDSVPTLAWTARADGWIDFYNRRWYE
                     YTGTTPEEMEGWGWQSVHDPVELPLVLERWRSSIATGEPFEMSFPLRGADGVFRWHLT
                     RVVPMRDAAGVIVRWFGTNTNIDAQLRTEKQLAEALAITETLNRIGSVLNTGLDLEAL
                     VQRLTDEATALCRAEFGAFFYNVINDRGESLMLYTLAGVPASAFARFPMPRNTLIFAP
                     TFRGEGVVRIDDVTKDPRFGKMAPHYGMPKGHLPVTSYLAVPVKAGTGEVIGGLFFGH
                     SRAAVFTARDEQLLTAVAAQAATAIENARLYEAERKARELAELGQSRTAKLAKITMSL
                     SRALTAEQTARCIIEEIASLLSATTGSVLLTAGDGRSVDRLVFHGAHSGRQPRTIDDD
                     RPVCETARTGTIIWVSGEEACRRYPQLRAEAQESGTITWGAIPLLFEGRTLGAIEVQF
                     STERPLAAEDAELMTTMGRQCGQALERARLFDAAGTARAEAENANRAKDEFLAMLGHE
                     LRNPLAPIVTALELMKLRHGTESSKEQDVIDRQVKHMTRLVDDLLDIAKITRGKVELD
                     RRPVELKSIIAKAFEIASPLFERRGHHVTVAEPDAPVWLHADETRLSQVLANLLTNAA
                     KYTDAGGTITVTARREARDVVIEVADNGVGIAPDMLPRVFDLFVQGYQDSDRRQGGLG
                     IGLSLVRNLVMLHGGTVSVASEGLGHGSQFVIKLPVLDVTTPNASPEQTQGRPSALRA
                     AGDHRRVLLVDDNRDAATLLADMLVTLGYEVVIAHDGPRALEVAHRFTPDVAILDIGL
                     PVMDGYELAARLRALTGAPVRLLAVTGYGQSDDRSRSEQAGFECHFVKPVNVTALIDA
                     IERRPSGGSR"
     gene            complement(11006..>11959)
                     /locus_tag="VMZ53_21615"
     CDS             complement(11006..>11959)
                     /locus_tag="VMZ53_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS31129.1"
                     /translation="FIRSIPGLERAEMTRPGYAVEYDFIDPREIWPTLETRRVQGLYF
                     GGQINGTSGYEEAAIQGFFAGANAAIALGFAPGGVTSEPLILRRDQAYGGVLVDDLTT
                     RGTKEPYRMLTSRAEFRLLLREDNAADRLMPRGRELGLIDDARWAQFGAWCAELAAAQ
                     ERAHGASVLGTDAVNAVLAKYGTAPIQNRRVSLAELYKRPELDHRSVEEVAAAGGIAA
                     WTGSEAALERASIECSYEGYLRRQEADAAKLEKADNVRLPDELDYRVIGGLSREVIEK
                     LEAVRPRSLGQASRISGVTPAAVAILMTHLSLQRRQSAAGE"
BASE COUNT         1988 a         4021 c         3905 g         2045 t
ORIGIN      
        1 gctcgccttc ggcagaacga agtactcgaa gagcccgccg agagaacgga caccgatcat
       61 cacgctgtag gtaggcagcg ggcggcaccc ttcgtgctcg taggccaccg gccccgcgaa
      121 cattccgaac gcgacacctg ctccatcgta atcgccgccc cacgcagcgc cgatcgtgcc
      181 gccgccggcg acggcatagg caagaccttg gtcgttccgc ttatcgacgc gcgccatgta
      241 tgcgggatcg aacgtgaggt aaccgcgact cggaccctgt ccggctgtga atccaccgct
      301 agtcacgaga ggcccggctc ctcctgagag gtccaaacca gcgcgaagcc catgaaccgg
      361 agacaccgcg atcgtcggtc caccgccgac gtgacacgca ctggcaagaa tcagaacaac
      421 gaggcagtac cgcatgcgcc caggatcgac aaatgccgat cgacgtggcg cccgttcacg
      481 cgttgctcac gcgtcgactc gcgttacgcg accgctcggt aaaccgcgac gttgtaggta
      541 gcggctaatc cgtcgctacg cggatcccgg cgaccgcacc gcccgcattg cgcagccacg
      601 ccggcacgag cttggggtgc agctcgacgg tgtcgggcac gaacgcagtg ccgagcgcga
      661 agtcgagcag cggattcgtg aggccgaagt tcacgcgcgc atcagcggcg tgatggtgcc
      721 agtggaaccg ccacatccac ttctcgtagc gcgtgcgcgg tgcgcgctcg tgcattcgcg
      781 cgtggtagca gttcactgcc acgaggcccg ccgtcagccc gacgctgagc ggcaacgtac
      841 gcgagagacc gatgaagggc gcgagcgcga gattgacgcc aaccagcgtc ttcgcgtagc
      901 gccccgcgtt gtcgcgcatc tcgctccaca cgtcgacggg ctgagccgcg acgcgatgat
      961 gctcgagatg ttgtgcgcga gtcgggtcgg cgttcttgcc gtgtgcgatc cagcgatgcc
     1021 aggccgattc gagcacgtgg gctgcgccga atcccgccgc ggcccaggtc atcgctctga
     1081 caatgcccac gtcctcaacc tacggcattg cgtcggatgg gtggcgccaa tgtgggggtc
     1141 tgaacgcgtc agctgctacg gcagcgtgtc ccagaagtcg cagagtgcgg ctttgtggcc
     1201 agtgcggacg ttgctcgtca catcgagcac gagcagctga tcgctcgctg tttcgaactt
     1261 cggccagtcg gcgccagggt ttcccgtcgc cgcgaacgtg gtccacgccg tttgcatctt
     1321 gtcggcaacc ggttggccgc ctgcgccgac cacgccgaac gggaacgagt cctcgccgaa
     1381 cacgaacgga atctccgacg cgtggaacac gccgagcccg gggagcaccg gattctcgac
     1441 ctcgcgctcg aagctgtagc gatacacgct cgcgcccgct gccgctgcgc cgcgcgctgc
     1501 acgtcgcgtc gggcagacga agaaaccatc gcccgtgatc tcggcgatcg cgcgatcgat
     1561 cgaggggaag cttgccggcg gatagtgcgc gaggatcgcg tcgacttgct cggacgtgaa
     1621 tcgggttgcg agcgcattgc gcatgtccga ctcgctcgtc gtttccttcg cgaccagcga
     1681 cgattggaac agcgtgcctt catcgcggtt gctgccgagc agcagcgggc gcggcatgaa
     1741 cgagcccgac gcgaatgcgt cgcgggagtt tttctcgacg acgaggccat cgacgttcgg
     1801 tgcgagcagg agaccatcgg gctggaggaa cgggccacct ggctgttggt cggagatctt
     1861 ggggccctgc accgcggtga gaagttgcgc caccgtcttg ccgcgcatgc acgtggccac
     1921 cgcggcgtca ttgcccgtgc acccgagcga cgttgcgaca cgtgcgccgg ttgcttctgc
     1981 ttcggctcgt gttggaaaga acaggttgtt gctgcacagg ccgctctctg cgatcgctgc
     2041 gtgaaagagg ccagcggtct tcgacgacgt gtactgccag cacgtgctga tgccgccggc
     2101 ggactcgccg aagacggtga cgtgcgtcgg gtcgccgccg aatgccttga tgttgcgttg
     2161 cacccactgc agcgccgcga gttgatcttc gaggccgtag ttgccggacg tcggatagcc
     2221 ggcgtcttcg gcggcgaggc cggcgtgcgc gaggaagccg aatgcgccga ggcgatagtt
     2281 caccgcgacg atgacgacgc cttgggtctc ggcgaggtgc tggccgttgt agtacgtctc
     2341 gccgccgctg ccgaatgcga acgcaccgcc gtggaaccag acgaggacgg gcgcgtcgtg
     2401 cttgtcgcgc gacgcccaga tattgagata gaggcaatcc tcttcgccgc tggccgcgcc
     2461 gaacgcggag aacacctgcg ggcacgctgc accgagctcg aacgtctcgc gcacgtcgtc
     2521 ccacgccgcg gcgggctttg gcttcttcca gcgcagctca ccgacgggcg gcgccgcata
     2581 cggaacgccg cgccacatgc gcgactcgcc ctgcacgagc cccttcacga cgcccgtatc
     2641 cgtcgagatc tggagtggct cggcgtcatc gctacagcca acgagcgcgg cgagccacgc
     2701 tacacacaac agcttcccca cgtgcatcgc gccactataa cgaaaaacgt cagcgcggcg
     2761 gatcgaacgc gatcacgacg atcgattttc cgttgtcgtg gtgcaggctg accgtggcga
     2821 acgtcgcatc gagcggagca cgcgctgcag acagccggcg aaatagcggc tcccagccgg
     2881 gcggcggcat gacaaagctc ggcgacgcga tcgcgaagcg cgggcccggt gctcgcggaa
     2941 gtcccgtcgc gcgatcggcg aagtagccct cgaggcggag cgtgtcggcg cgcgtgtccc
     3001 acgtcgctgt cgcgcggacc ttcgtcgaga tgcgcgtcgt gaggttcggc gccgagtagc
     3061 cgaccggcgt gcgcggagga cgcgtcgcca acgcggcatg ctccgccgcc tccggcagct
     3121 cgaacacgtg cggcgcgccc gccccgacgc cgagcagcgc cgcgagaaca cgccccggct
     3181 cgtcgcgaaa gacgaacgac atctcgacga tgtctggcgc tcgcgccatc cagcgtcagc
     3241 gcttactcgg gcgcgtcgcc gccgagcttc acggcgggga acagcgcggc ggtcttcatc
     3301 gcggcgtcca tctgcgtatc ggtcagcccg aacgtcgcgc gcatgttggc gaggaactcg
     3361 acctcgcgcc aatccgacac gccgtcggcg agcgacacga tcatcgtgta gcaggtcacc
     3421 cagtagcgat cgtttccggc ggtcacctcg gacgcgacgc tctcgagctc cttcttgaga
     3481 tccttgcccg cgaccctctc gagcgctacg tcgatcgctt cgtcgatctc ggcgtgcgtg
     3541 agcacggcca tgtcgggaat cgcgcggagg atcgagcgca tcgcaaggcg ctcttcgtgg
     3601 tggatctctt cgtcggccga cgccatgagc gccatgcagc gcacgaacat cgggagcagg
     3661 aagcgaacct tctcgctctt ctcctcgagc gtcagcaggc cggagtgctt gcgcgcggct
     3721 tcgaagacgt ccttcgtctc ggagtcgtcg agcacgaacg ccttctgcat cgcatcgagg
     3781 aactcgatct cggtttcggc gaggttcttg tcggaggcgc aaatctcggc agcgagccca
     3841 tacgcggtga ggcgctggcc gcgcgacggc aggccctcgg cgatcgcggg aagacgcgcc
     3901 ttgccgccac ccgcgacgcg cagcgcatcg gctgcgagat cggtcagacg cgagagctcg
     3961 tcagcagaca gcccctcgaa catcttgtgg ttggcgagat tgccctcgaa cgccgccatc
     4021 tcttcttcgg agatgtcccc atcggcttcc atcgcgccga gcatcgcttc gaccaaaaac
     4081 cggcgcgagt cttcgccgga cacacgaccg ctgaacatct tccgtagaaa gctcatcgct
     4141 gactacggta ccgctagccc atctgtttga tccacttgcc ggggtcgcca atacgcggct
     4201 tctcacctcc gcgaacgagg tagcgcaccg cgtgattgag accgtccaag gtcatcgcaa
     4261 acatcgggaa caccgacgcg attacctcga tcgtcgtgcc agaccagaat ctttctggct
     4321 cggggttgag ccacgccgag ctgcggaaat gatgtgcgag gcggcggagc cactcgatgc
     4381 cgggcgcacg cgtctgcgag tacagatacg acgagccgta cgacatcatg ccgccgtcga
     4441 gcagctccgc gggatgcatg agtgcgtcgc cgagcatcac gagcttctcg tcgcgatcgc
     4501 tgttcgcgag gaggtcggcg accggcacgc ccttgcggaa ctgcgcatcc tcgtagaccg
     4561 agttgtagac gcagttgtgg aagtagtagc tgcgaaactt tgcgaagcgt cccgcgcgag
     4621 acgcggcggt gaacagacgc gacgcgagct ccgcgtgcgg atccatcgag ccgccgacgt
     4681 ccatcagcag cacgaccttc acgcgattgc gacgcggcgg gcgaaatacg atctcgagct
     4741 cgccggcgtt cttgcccgtc ttctcgatcg tgtcgtcgag gtcgagctct tcttgcgcac
     4801 cctcgcgacc gaggcggcgt agcccgcgca gcgccatgtc gatctggcga acgtcgagca
     4861 cgacatcgcg gcggtattct ttgaagaggc gctgatcggc gaccgccatc gcgctgcggc
     4921 caccaccgcc accgacgcgc atccccgtcg gattcatgcc gcccgtgccg aatggcgacg
     4981 tgccgccggt accgacccag cggttgccct tgtcgtggcg ctctttctgc tctttgaggc
     5041 gcttctcgaa ctcggcgcgc agcttctcga agtcgagctc gctgagcgct ttgcgctgct
     5101 cttcggtgag gccttcgagc gcctgcggat tcatcagcca ctgctgcagc tgatccgtga
     5161 gcttgatcga atcgacgtgg atgtctttga agtacgcgag gaacgcctcg tcgtactgat
     5221 cgtagaacgc gatgtccttc acgcagatcg tgcggcacag gtgatagaag ccgtcgagcg
     5281 acgactcgtg cagaccgagc gccatcgctt ccatcagcgc catccattcg tgcgtcgaca
     5341 ccggaatctt ccgctgccgc agctcgaaca gaaagtcgat cagcatttag gccttcgccc
     5401 tcttctggac gagctcgaca tcgctctcgg tcttgagcaa cgtgccgagg aatggaatgc
     5461 cgtgaccgac cttcgcgaga tcgacgccgc ccttcttgag cgccatgatc caatcgatga
     5521 gctcggacgt cgacggcttc ttacgcaagc gcggcgtcgc acgcagcccg aagaacagct
     5581 cgagtgcgtt gtcgagcaca cggtccgtga tgtcgggatg gtgaacgcgc acgatctccg
     5641 tcatcagctc gcgatcggga aactggatga agtggaacac gcagcggcgc aagaacgcgt
     5701 ccggcagctc cttctcgttg ttcgacgtga tgacgatgac cgggcgctcc ttggcgacaa
     5761 cttcttcgcc ggtctcgtcg atgcgaaatc gcatcgcatc tagctcgagc aagaggtcgt
     5821 tcgggaactc gatgtcggct ttgtcgatct cgtcgatcag cagcacgact ttgctcggcg
     5881 acttgagcgc ttcgccgagc gggccgagct tgatgtactt ggcgatgtcg cgaacgtcgc
     5941 caccgaagcg cgaatcgtgc agacgcgcga cggtgtcata gacgtaaagg ccgtccttgg
     6001 ccttcgtcgt cgacttcacg tgccagcgaa tcagcggcag gccaagcgac gccgcgaggt
     6061 tctccgcgag cagcgtcttg ccggtgcccg gctcacctcg cacgagcagt gggcggcgaa
     6121 gcgcgaccgc gacgttgacc gcgtgcctca gctcctcgga ggcgatgtat gtagcggtgc
     6181 ctttgaactg atcgaactgc cggtcgtcgt cggacatcta gccgaccata gcacccgtta
     6241 ccgcttgccg cgcttgcccg ttacgtaggc gtcaccgacg ccgtaaagcc acgtcactgc
     6301 accggcgacg gcaaagatgc cgccgacgag cgggatcgcc gaagcgatac cgcacgcgac
     6361 cgacaccgcg aacacgccga gcgctttact gccctcgccg ttcacgagct gaccgacgcc
     6421 tggaatgagc gcgctgcaga cggccggaac cacaccgctg ccaccacccg acgactgacg
     6481 catcaacgcc ttcgacatac ggcctcctag ggctccacca ctagaaccac gttgacacac
     6541 cgagcatgaa gagcaagtag tgactcttga acgtgccctg attgaccggg ttctccagct
     6601 ggctctgcgg cgtgttcacc gggttgatcg ggtcgggacc ctggcgatcg tcgatggcct
     6661 gctggatgtc accggcgccg cagccggagt acggcacgtt ctgcggcggc gagctctgct
     6721 tcgatggatc tgcaatcggg ttgcacgtgc gcgagtccgt gcgcgtacct tcgagaaccg
     6781 cgccgaagcc ggcatcgatc tgaacgcgcg acatcttgta cgaggctccg gccgtcagcg
     6841 tcgtgcgcgc cgagccgtcg atgtccgcgc gctcccagcc cggcttcgcc gccgcggtgt
     6901 cgtacgcgac gccaccacgc gcgatgactt cgttggcgcc cgccgcgaag tggtagctgc
     6961 cgccgaggcg aacgccgtac gtatcgcgga agccgtgcgc gacgatgttg tccttgagat
     7021 cgatgccgtt gtccggcgcc gactccgtcg cgacctgcgc atcgacgacg acgcggtaat
     7081 cgctcgggct gtggcagccg ggatccgcga cgtagtcgca cgtcttgccc cagtgctccc
     7141 acgtgacgtc gagctcgacg tcgcccttct ccttgtcgtt cgcgtcggtg agcttgtagc
     7201 ggccgccgac agtcgcggtc atcggcagcg cgaactgcac gcagcccttc tgcgcctcgt
     7261 cggtgccacc cgctgcacaa cgcgtcgcgg cgctcggccg gacgtggaac ggctcgccgt
     7321 tgagcgagac gcccggaccg ttgaccgcgt gcgcatcgcc cttcgcggtg acgttcgtct
     7381 gcggcgtgaa gttgaggccg agctcgatgt tcttgccgac gcggtacgcc gcgcctgcac
     7441 cgagcgccat cacgaagctg tccttcgcct cgagcgtgaa cagaccgtct gccttcaccc
     7501 actcgacgat gtttgcgggc gagccccaca ccgcgatcgt cgacttcacg tcggcaaagc
     7561 cggccgaaaa gcgcacgccg atgtcgaggt cgggaatcac gcggtacgcc gcgacgatcg
     7621 acggcaggat gaccgcgccc tcttgcgcga tgatgtcgta gcgcgacgga ggcggtggcg
     7681 cgccgaacgc cgggtactcg taggcgccgc cgctctcgac gtagtagttc tggccgccga
     7741 cgttattcat gcggcggaac ggatacgcgt tcggcgcgta gagcccgaaa ccgacgctga
     7801 ggtttggcac cttgccgccg agatccgaca cgatgccgat gacgggcacc ggctggtagc
     7861 gaccgatgcc gagcggtggg gccgcgtctt gcgtgacgat cggatagcgc tggccctcgt
     7921 acgactctgc gtcgttcggg ttgtcgtcgt acgtcccgtt gcgctggaac gtcatgaagt
     7981 agctgatcgc cgccgcgccg atcgtgatct tcgtgcccgt cgtcttcgcg atgcctgctg
     8041 ggttgaggcc gatcgactcg gcgtcttccg tcgcagcgac gctcgcacct gcacgcgagg
     8101 tcgagaccgc accaccaccg ggcaggatca ggccgccggc cgctgccgta cctgaaatgc
     8161 acgagaccac cgtcaacgtg aggccaccaa gcttcatagg gccccgacgc tacatcacgg
     8221 ccgcccgctt ttcctctgcg aacgtgagtc tattacccgg attcgcgggc ggcgatgttg
     8281 ctctagatgg aggcgtacga ggtgatgttc tagcgggagc cgccgctcgg acggcgctcg
     8341 atcgcgtcga tcaacgccgt cacgttcacc ggcttcacga agtggcactc gaagcccgct
     8401 tgctcgcttc gcgagcgatc gtcggactga ccgtagccgg tgacggcgag gagccgtacg
     8461 ggcgcgccag taagcgcgcg caacctcgca gcgagctcgt atccatccat caccggcaac
     8521 ccgatatcga ggatcgcgac gtcgggggtg aagcggtgag ccacctcgag cgcacgcggc
     8581 ccatcgtgcg cgatgacgac ctcgtagccg agcgtgacga gcatgtcggc gagcagcgtc
     8641 gctgcgtctc gattgtcgtc gacaagcagc acgcgacgat ggtcgccggc ggcgcgcagc
     8701 gcactcggtc gtccctgcgt ttgctccggc gaggcgttcg gcgtcgtcac gtcgagcacg
     8761 gggagcttga tcacgaattg actgccgtgg ccaagcccct cgctggcgac gctgacggtc
     8821 ccaccgtgca acatcacgag attgcgcacg agcgacagcc cgatgccgag cccgccctgt
     8881 cgccgatcgg agtcctggta accctgtacg aacagatcga agacccgcgg cagcatgtcg
     8941 ggcgcgatac ccacgccgtt atcggccacc tcgatcacga cgtcgcgagc ctcccgacgt
     9001 gcagtgacgg tgatcgtgcc gcccgcgtcg gtgtacttcg cggcgttcgt gagcaaattg
     9061 gcgaggacct gtgagaggcg cgtctcgtcg gcgtggagcc acaccggcgc gtccggttcg
     9121 gcgaccgtga cgtggtgacc gcgccgctcg aatagcgggc tcgcgatctc gaacgccttg
     9181 gcgatgatgg acttgagctc gacggggcgg cggtcgagct cgaccttgcc gcgcgtgatc
     9241 ttcgcgatgt cgaggaggtc gtcgacgagc cgggtcatgt gcttgacctg gcggtcgatc
     9301 acgtcttgtt ccttgctgga ctcggtgccg tgccggagct tcatcagctc gagcgcggtg
     9361 acgatcggcg ccagaggatt gcggagctcg tgccccagca tcgcgagaaa ctcgtctttg
     9421 gcgcgattcg cgttctcggc ttcggcgcgt gcggtgcctg cggcgtcgaa cagccgggcg
     9481 cgctcgagtg cttgcccgca ctgacggccc atcgtcgtca tgagctctgc atcctcggcg
     9541 gcgagcgggc gctcggtcga aaactggact tcgatcgcgc caagcgtgcg cccctcgaac
     9601 aggagcggaa tcgcgcccca cgtgatcgtg ccggattcct gcgcttcggc gcggagctgt
     9661 gggtagcgcc gacaggcttc ctcacccgat acccagataa tcgtgccggt ccgcgcagtc
     9721 tcacagactg gtcgatcgtc gtcgatcgtc cgcggctggc gtcccgagtg cgcgccgtga
     9781 aacacgagcc ggtcgacgct gcggccgtcg ccagcagtca gcagcacgga gccggtcgtc
     9841 gccgacagca gcgacgcgat ctcctcgatg atgcaccgcg cggtctgctc cgcggtgagc
     9901 gcacgcgaca gcgacatcgt gatcttcgcg agcttcgccg tgcgtgactg gccgagctcg
     9961 gcgagctcgc gggccttccg ctccgcttcg tagaggcgcg cgttctcgat cgcggtcgcc
    10021 gcctgtgccg cgacggccgt cagaagctgc tcgtcgcgcg ccgtgaatac ggccgcgcgt
    10081 gagtgaccga agaacagccc accgatcacc tcgccggtgc ctgccttcac ggggactgcg
    10141 agatagctgg tgacaggcag gtggcccttc ggcatcccat agtgcggtgc catcttgccg
    10201 aaccgcggat ccttcgtgac gtcgtcgatg cgcacgacgc cctcaccgcg aaatgtcggc
    10261 gcgaagatca gtgtgttgcg cggcatcgga aacctcgcaa acgctgacgc gggcacaccg
    10321 gcgagcgtgt agagcatgag gctctcaccg cgatcgttga tgacgttgta gaagaacgcg
    10381 ccgaactccg cgcggcagag cgcggtggcc tcatcggtca gtcgctggac gagcgcctcg
    10441 aggtcgagac cggtgttcag cacgctcccg atccggttga gcgtctcggt gatcgcgagg
    10501 gcctcggcga gctgcttctc ggtgcggagc tgtgcgtcga tgttcgtgtt ggtcccgaac
    10561 caacgcacga tcacgcccgc cgcgtcgcgc atcgggacga cgcgcgtgag gtgccagcgg
    10621 aagacaccat cggcaccgcg cagcgggaat gacatctcga acggctcgcc ggtcgcgatc
    10681 gacgaccgcc atcgctcgag caccaacgga agctcgactg gatcgtgcac ggactgccag
    10741 ccccagccct ccatctcctc gggtgtcgtg ccggtgtact cgtaccagcg gcgattgtag
    10801 aaatcgatcc agccgtccgc gcgcgcggtc cacgctagcg tcggtaccga gtcgatgagc
    10861 aggcgagcca gcccatcgtc gaacgggccg ccagatgatt cgtccgccac gctggaagtt
    10921 tgcacgatct ttcacccctt cgacgaaggg tcgcgcgtca cgacgatgag cccaaacgct
    10981 cgctgtgcgc gtacgcacgc cgccctcact cgccggcagc agactgtcgt cgctggagcg
    11041 agaggtgcgt catgaggatc gcgacggcag caggcgtgac gccgctgatt cgcgacgcct
    11101 ggccaagcga tcgcgggcgc accgcctcga gcttctcgat cacctcgcgc gataggccgc
    11161 cgatgacccg gtagtcgagc tcgtcgggga gccgcacgtt gtcggccttc tcgagcttcg
    11221 ccgcatcggc ttcttgccgc cgcagatagc cctcgtacga gcactcgatg ctcgcgcgct
    11281 cgagcgccgc ctcgctgccg gtccaagcgg cgataccgcc tgccgcggcg acttcttcga
    11341 cggagcgatg gtcgagctcg ggccgcttgt agagctcggc gagcgagacg cggcggttct
    11401 ggatcggcgc cgtgccgtac ttcgcgagca ccgcgttcac ggcatcggtg ccgagcacgc
    11461 tcgcaccatg cgcacgctct tgcgccgccg cgagctccgc gcaccacgcg ccgaactgcg
    11521 cccaccgcgc gtcgtcgatg aggcccagct cgcggccgcg cggcatgagg cgatccgccg
    11581 cgttgtcctc gcgcaggagc aggcggaact ctgcgcgcga cgtcagcatg cgatacggct
    11641 ccttcgtgcc gcgcgtcgtc agatcgtcga cgagcacacc gccgtacgcc tgatcgcggc
    11701 gcaggatcag cggctcgctg gtcacgcccc ccggggcgaa gccgagcgcg atggcggcat
    11761 tggcgccggc gaagaagccc tggatcgccg cctcctcgta gccgctcgtg ccgttgatct
    11821 gaccgccgaa gtacaggcct tgcacgcgac gcgtctcgag ggttggccag atctcgcgcg
    11881 gatcgatgaa gtcgtactcg acggcgtagc cgggtcgcgt catctccgcg cgctcgaggc
    11941 ccggaatcga tcgaatgaa
//
LOCUS       DATLBZ010000426        10944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9953971, whole genome shotgun sequence.
ACCESSION   DATLBZ010000426 DATLBZ010000000
VERSION     DATLBZ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9953971"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1863
                     /locus_tag="VMZ53_21620"
     CDS             <1..1863
                     /locus_tag="VMZ53_21620"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31130.1"
                     /translation="GDSITFTNPATAKACTASFVATVNVSASAGTGGTVAAGAPAGGT
                     CTGTNCTLDSGASSVTFTASPIANYRFGSWSGATCTGGTPSGSAITFTNPTSAKACIA
                     NFVATVAVSASAGTGGTVAAGTPTGGTCSGTNCTLDSGASSVTFTAQAGTNYRFSSWS
                     GSTCTGGTPSGTAITFTNPTSAKACTANFVATVSVSAAAGTGGSVAASGLTGATCSGT
                     SCTLDNGASTVTFTPSATSGYRFNGWSGTTCTGYSQSGNAITFTNPTTAKACTASFVA
                     TVVVAGAAGTGGSVSVFSYSGGSCAGTTCTLDSGASSVTFQATPSANYRFSSWSGCSL
                     STSSQVTITNPTISTTCTANFVATVVIDAVPAPAFGGSVAVTSLSNGTCSGTSCTMDS
                     GSGSVQFTPTPASNFRFAGWSGGTCFGYFSGAGNSIIFSGATASHSCVAEFVATVTVE
                     AAPTPTGSGSVTVDNLSGGTCVGSSCVLDSGSSSVTFWPEPAPGYRFVKWTGASCSGY
                     IAGQDNEIIFIDPTSSHGCRANFEQAVTVQVSSIIGNGSVVVTTMTNATCTNLTCLVS
                     TGAGTVTFTPVPGGPNSPFQKWLGNSCNGTVANDGSMTFTNPTSDHDCQAYFSP"
     gene            1882..2673
                     /locus_tag="VMZ53_21625"
     CDS             1882..2673
                     /locus_tag="VMZ53_21625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS31131.1"
                     /translation="MSTRDYSTISPSARAVLAMRSLSDELPFVRAAAEIVMGRDALAE
                     EHARLQALVGWQMRLWHFVERYKSIDVMLAARPEKIVVELAGGLSLRGLALTQRQAIT
                     YLDTDLPEMIETKRSVVDQVATGPLAGELTLRALDALADGALAAAVDELPAGPVAIVN
                     EGLFMYLDDAEKQRLAAAVRGVLAQRGGAWITADIYLKTPAQMPPIGQDDKLKHFLTT
                     HRVEENKFESYAAAAKLFTEAGFAIAKREGASPIRQTWMLEVAGS"
     gene            2694..3161
                     /locus_tag="VMZ53_21630"
     CDS             2694..3161
                     /locus_tag="VMZ53_21630"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HUS31132.1"
                     /translation="MADRLRARAHAALERVPGKVGEKLRSANEALGRPFAEESELEER
                     REFEARQSKPGSAPAAAPAPKPAAGDAAPVIVYYMEKQKRDVSKLTDILDANGIKYTV
                     TNIQEDPAAQYAVKRDSKGLRLPVVFVAGDCVGGREQLVNAASSGELKKKVFG"
     gene            3455..4132
                     /locus_tag="VMZ53_21635"
     CDS             3455..4132
                     /locus_tag="VMZ53_21635"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HUS31133.1"
                     /translation="MSTTDQDKPKWTKQATEKVFAALNRADELGGEIRDFLTDKVAAD
                     PRYVNARKRVAKLFGGTYESKTEVAQKATAAEAKRKAVAAPSPPTKKEAAAAAASKGF
                     GNPEIKAQVFGKKSCPWSGRSITILERNKVDFDFVDLEEPEHESKLAGLQLETKQHTV
                     PFNYLRGQFIGGFNALAEIERLGQLEFAMMTKEERANAPAHVRGVEIVARPNTDEVVP
                     ADETPGE"
     gene            4272..5525
                     /locus_tag="VMZ53_21640"
     CDS             4272..5525
                     /locus_tag="VMZ53_21640"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HUS31134.1"
                     /translation="MQLKLLAGAVLTVATAAGCVDEPNYGSTQGLSFEEFKARTYREP
                     GTGLYVLDWDMPVSGDAQLYQIWEATQQGTLAVMNINGQDIIWTATQRKQLTYCISNT
                     FGANKQTVIDAMAKASDLGWEKMADVNFTYVSTQDGNCNAQNTQVLFDVNPVNANGQY
                     LARAFFPNSTRAERNVLIDTTAFQPGGTGNVPLPNIISHELGHTLGFRHEHIRPEANA
                     TQCAEDTQFRGLTTYDAASVMHYPQCNGTSQDLAFTQKDKDGVVALYGAPANNPSPMT
                     QVTAPADNATVGPNFDVVASIIDTDLMKAELKIDGTLKDTLTAAPYTFHISALAVGAH
                     TLEITATDGAGQKTTQTLHLTVAQNGGGGGNGSGGGTGTGGGDGLDDSNVYGGCDATG
                     GGGAAGGGLLALIAFASLVRRRRTV"
     gene            5536..6600
                     /locus_tag="VMZ53_21645"
     CDS             5536..6600
                     /locus_tag="VMZ53_21645"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HUS31135.1"
                     /translation="MKWWLAALAVALAFEKPAEPCAIAPPDTSARIQIVDEEALIIWD
                     PATKTEHFIRRAQFASDAKAFGFLVPTPTPPKLTELDAEVFAILADRTAPQVEYRTER
                     PWQLGAWLLEGCMLMKGDKAMPAGAPIDSGVRVLSTTNVAGYDATALQASDPSALGAW
                     LTAHGFVMTPAIEQWLAIYVQKQWTMTAFVVAGAESTHGDVTTRAIDMTFQTDTPFYP
                     YREPGAGTELDGVTSAPGRSLRVYFAAPQRHAATLATKPWAAKVSYAHEFSGVPGVEA
                     LLGASRYITAFIDETSPRRGIDELYFAPSVTGDVVPPPFVVTDREPRLIPVDMILIVG
                     LFGLWIVRRLRRIRRRARAS"
     gene            6720..7589
                     /locus_tag="VMZ53_21650"
     CDS             6720..7589
                     /locus_tag="VMZ53_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31136.1"
                     /translation="MATESTAIVNLLDLVKNQKPLDRDSADDMLFGGRVRFDPMAMPA
                     PAQPVMLQPIPSLAQGTPVSVPLPQPFVRPDPKATLAKFALPVTLFCCVMVLLSVYLW
                     KGESKSTPAPKATIVHAPPPAPVAHVMPEAPPQPTVTEIQPTVPTVPEMQPTVPTVPT
                     VPTVAAADEAQDDAAADEAKPVAKSAKSKRSKHHSRAAKRSKKETRVAVAEAPKAKPE
                     KLGGKGALQLSSSPAREVWVDGKNTKQSTPQKLTLAPGTHNITLFDKKTRSAKTFQVE
                     IKPNETTRVSKKY"
     gene            complement(7586..8617)
                     /locus_tag="VMZ53_21655"
     CDS             complement(7586..8617)
                     /locus_tag="VMZ53_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31137.1"
                     /translation="MYRDPAFVEVTDTTSSEGPPPIAIWGYFSRLLACDRGRDPLLPI
                     RSLVYTGEPNMDEHYVRRVSLGIGGYLRDWDEKLVDRDVYSNGFVGRIGDLVIIPRTN
                     AVAARELETGNVRWELAHPAKLHRAPMRRGDDEVFLAFADRTWLRVTAADGTVCERGA
                     VRSEEELDALVEKATELSGRHGDAIWYGGHTLKAWGGVYVQEDDDPSRSIVRYERAAP
                     AVTGDIAIDGWEADDYIDLIGDSLVVWLKRTWGDTNHAALAFVDPTSIALRSILELGP
                     IRSFRSAYRVDGLLVITVEGLPPPGGHRGAPTDFIVDPKAERLLARFCEGRSVVFDDA
                     GTEAWTAAL"
     gene            complement(8620..10017)
                     /locus_tag="VMZ53_21660"
     CDS             complement(8620..10017)
                     /locus_tag="VMZ53_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31138.1"
                     /translation="MKTTIAAFLLLTSVASADEQAPTEETKPQEEKKEEVKTAAIEQP
                     TTTESVSISKDAPQGTTKAVEVGETRFELHGYARMPLAAQGTPREPYLVDNDYYLSGF
                     AYTRLYEPDWSELFFSAKHGDYEAKFGLFASLYSDYATARLENQLGVAQASVSAKHFA
                     GVDPLSVQVGVFWDRFGYIEPYDTYLYGRTHQGGAKVAYELPGGGRIQTGIGIHQAQL
                     QQNLGLTPVAHIAGAYPVGPVELGAYTLRTWTRDKRQLSPIQDGTMYVAGLDARYKLP
                     EDRGTAYLAFGYYHMDHVLYLAPALEVMHSTGGRGLTENYLGLDASEDGSGHMYTLAT
                     DVKTNITDQVGLRVFGMATWVRSPQVDEMDPQINRDRRLYLKWGLDPSFLVLPKLRVS
                     ARFDRVILDMYDSENSFRVLSPRITFPLDTWGELFFMYSHYWYGDKVRLRPGQVPLET
                     MPDANVFKLQAQVVW"
     gene            complement(10019..>10944)
                     /locus_tag="VMZ53_21665"
     CDS             complement(10019..>10944)
                     /locus_tag="VMZ53_21665"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUS31139.1"
                     /translation="LQEERYQKDVKYFEARAKDLGFRVVTLAADNDNAKQIAQVEDVL
                     TQGVKVLVIQPTDSQAASSYVKLAHEKGAKIIAYDRAIVAPDLDYYVSHDSYRVGVLQ
                     AQAALAATGNKGKYVILSGQAGHSVATEITRGYKDTLAPYVAKGDIEIVLEQNHSSWS
                     PEQALRTVEDALTRSSNHVDAILANNSGMARGAVQAVNAAGLDHVFIAGADADAANVN
                     FVCQGKQAIEVLKDIQPLAKTAADVSKQLLDGTTPAAGSSIALAGGNIPVAAVRVEVV
                     TPDTVKALVVDTGFLSADELPACKSRLAAVH"
BASE COUNT         1961 a         3556 c         3655 g         1772 t
ORIGIN      
        1 ggcgactcga tcacgtttac gaacccagcg accgcgaagg catgcaccgc gagctttgtc
       61 gcgacggtca acgtctcggc gtcggcaggt accggcggca cggtggcagc aggagctccg
      121 gcgggcggca cgtgcaccgg cacgaattgc acgctcgaca gcggcgcgag cagcgtgacg
      181 ttcacggcat cgccgatcgc gaactatcgc ttcggcagct ggtccggcgc gacgtgcacc
      241 ggcggcacgc cgagcggcag cgcgatcacg tttaccaatc cgacgagcgc gaaggcatgc
      301 atcgcgaatt tcgtcgcgac ggtcgccgtc tcggcgagcg cgggcaccgg cggtacggtc
      361 gctgcgggca caccgacggg cggcacgtgc tcgggcacga actgcacgct cgacagcggc
      421 gcgagcagcg tgacgttcac ggcgcaggca ggcacgaatt atcgcttcag cagctggtcg
      481 ggctcgacct gcacgggcgg caccccgagc ggcaccgcga tcacgtttac caatccgacg
      541 agcgccaagg catgcaccgc gaacttcgtc gcgacggtat ccgtctcggc ggccgcaggc
      601 accggcggct cggtcgcagc gtcgggcctc actggcgcaa cctgctcggg aacgagctgc
      661 acgctcgaca atggcgcgag caccgtcacg ttcacgccgt cggcgacctc gggctaccgc
      721 ttcaacgggt ggtcgggaac gacgtgcacg ggctactcgc agagcggcaa cgcgatcacg
      781 ttcacgaatc cgacgaccgc aaaggcgtgc accgcgagct tcgttgcgac ggtcgtcgtc
      841 gcgggcgcgg caggcaccgg cggcagcgtc tcggtcttct cgtatagcgg cggcagctgc
      901 gcaggcacga cgtgcacgct cgacagcggg gccagcagcg tgacgttcca ggcgacgccg
      961 tcggcgaact accggttctc gagctggagc ggttgctcgc tgtcgacgag ctcgcaggtg
     1021 acgatcacga atccgacgat ctcgacgacg tgtaccgcga atttcgtcgc gaccgtcgtg
     1081 atcgacgctg tcccggcacc ggccttcggc ggatccgtcg cggtcacgag cctgtcgaac
     1141 ggcacatgca gcggcacgag ctgcacgatg gatagcggca gcggcagcgt gcaattcact
     1201 ccgactccgg cctcgaactt ccgcttcgcg ggctggtccg gcgggacgtg ctttggctac
     1261 ttctcgggcg ccggcaactc gatcatcttc agcggcgcga cggcgtcgca ctcctgcgtc
     1321 gcagagttcg tcgcgacagt gactgtcgaa gccgccccca cgccgacggg ctctggctcg
     1381 gtcaccgtcg acaatctctc cggcggcacg tgcgtcggct cgagctgcgt gctcgacagc
     1441 ggctcgagca gcgtgacgtt ctggccagaa ccggcgcccg gctatcgctt cgtcaaatgg
     1501 accggagcga gctgcagcgg gtacatcgca ggccaggaca acgagatcat cttcatcgac
     1561 ccgacgagct cgcacggctg tcgcgccaac ttcgagcagg cggtgacggt ccaagtgagc
     1621 tcgatcatcg gaaacggctc cgtcgtcgtg acgaccatga caaacgcgac gtgcacgaac
     1681 ctcacgtgtc tcgtcagcac cggcgcgggc accgtgacgt tcacgccggt cccgggcggc
     1741 ccgaattcgc cgttccagaa gtggctcggc aactcgtgca acggcaccgt cgcgaacgac
     1801 ggctcgatga cgttcacgaa cccgacgtcg gatcacgact gccaggcgta cttcagcccg
     1861 tagcgcggct acgctaatgc agtgagcacg cgcgactact cgaccatctc tccgtcggcg
     1921 cgggccgtgc tcgcgatgcg ctcgctcagc gacgagctgc cgttcgtgcg cgccgctgcg
     1981 gagatcgtga tgggacgcga cgccctcgcc gaggagcatg cgcggttgca ggcgctcgtc
     2041 ggctggcaga tgcgcctgtg gcacttcgtc gagcgctaca agagcatcga cgtgatgctg
     2101 gcggcgcggc cggagaagat cgtcgtcgag ctcgcgggtg gactctcgct gcgcggcctc
     2161 gcgttaacgc agcgtcaagc gatcacgtat ctcgataccg acttgcccga aatgatcgag
     2221 acgaaacgga gcgtcgtcga tcaggtggcg acgggcccgc tcgccggcga gctcacccta
     2281 cgcgctctcg atgcgctcgc cgatggcgcg ctcgcagctg cggtcgacga gctgcccgca
     2341 gggccggtcg cgatcgtcaa cgagggcctc ttcatgtatc tcgacgacgc ggagaaacag
     2401 cggcttgccg cagcagttcg cggcgtgctc gcgcagcgcg gcggcgcatg gatcacggcg
     2461 gatatctatt tgaagacgcc cgcgcagatg ccgcctatcg ggcaggacga caagctgaag
     2521 catttcctca ccacgcatcg cgtcgaggag aacaagttcg agagctatgc cgcggccgcg
     2581 aagctgttca ccgaggcggg ctttgcgatc gcgaagcgcg agggcgcatc gccgatccgc
     2641 cagacgtgga tgctcgaagt cgcgggctcg tgatatgcgt cgtcgatatg agcttggctg
     2701 atcgactcag ggcacgtgca cacgccgcac tcgagcgagt gcctggcaaa gtcggcgaga
     2761 agctgcgcag cgcaaacgaa gcgcttggcc ggccattcgc ggaggagagc gagctcgagg
     2821 agcgtcgcga gttcgaagcg cggcagtcga agccgggcag tgcgccggcc gctgcgcccg
     2881 ctccgaaacc ggcggcaggc gatgccgcgc ccgtgatcgt ctactacatg gagaagcaga
     2941 agcgcgacgt ctcgaagctg acggatatcc tcgatgcgaa cgggatcaag tacaccgtca
     3001 cgaacatcca ggaagatccg gccgcgcagt acgcggtgaa gcgcgacagc aaaggcctgc
     3061 ggctacccgt cgtgtttgtt gcgggtgact gcgtcggagg tcgcgagcag ctggtgaatg
     3121 cagccagctc gggagagctc aagaagaagg ttttcggcta gcgacgcagc gatcagatca
     3181 cgccgaggag gatccgagtg gccatccacc cgatgactcc agaggcgaga gcggtcacag
     3241 cagcgctagt catgacgagg ccaagtcgac gcataggggc cctaactagc atgggccgtt
     3301 ccacgtttac cagtgcgtaa catcaactag ttgcgcgtcg atccctgggt ccgattgttc
     3361 accgggcgac tcgcacccac ccacggaccc ccagtggggc caaccgtccc attctgctct
     3421 gaaaagcgcc gatgtcgtga tacggaagcg tgcgatgtcg acgaccgacc aagacaagcc
     3481 gaagtggacg aagcaggcga ccgagaaggt cttcgcggca ctcaaccgag cggacgagct
     3541 gggtggcgag attcgcgact tcttgacgga caaagtcgcc gccgaccctc gctacgtgaa
     3601 tgcacgcaag cgcgtcgcga agttgttcgg cggaacgtac gagtcgaaga ccgaggtcgc
     3661 gcagaaggcg acggcggcgg aggcaaagcg caaagcggtt gcggcgccgt caccaccgac
     3721 gaagaaggaa gctgctgcgg cggctgcgag caagggtttc ggcaatcctg agatcaaggc
     3781 gcaggtcttt ggcaagaaga gctgcccgtg gagcggccgt tcgatcacga tcctcgagcg
     3841 caacaaggtc gacttcgact tcgtcgatct cgaagagccg gagcacgaga gcaagctcgc
     3901 gggtctgcag ctcgagacga agcagcacac ggtgccgttc aactacctgc gcggtcagtt
     3961 catcggcggc ttcaacgcgc tcgccgagat cgagcgactc ggtcagctcg agtttgcgat
     4021 gatgacgaaa gaagagcggg cgaacgcgcc ggctcacgtg cggggcgtcg agatcgtcgc
     4081 gcgcccgaac acggacgaag tcgttcccgc cgacgaaacg cccggcgagt agttgcacaa
     4141 ctagcgcaac atcgctgatc ccctggggga gttggtttcg ctcccactca ccagcgaacc
     4201 acccgcgcat ggctaagtgc gcgtgccggc tggcgtgact gctggcacta gtcctgtaac
     4261 tgggagtagc catgcagttg aagttgcttg ctggcgcggt cctcacggtg gcgacggcgg
     4321 cgggttgcgt cgatgaaccg aactacggct cgacgcaggg cctctcgttc gaggagttca
     4381 aggcgcgcac gtatcgcgag cccggcaccg gcctctacgt cctcgattgg gacatgccgg
     4441 tcagcggcga cgcgcagctc taccagatct gggaagcgac gcagcaggga acgctcgccg
     4501 tgatgaacat caacggccag gacatcatct ggacggcgac gcagcgcaag cagctcacgt
     4561 actgcatcag caacacgttc ggcgcgaaca agcagaccgt catcgatgcg atggcgaagg
     4621 cgagcgacct cggctgggag aagatggccg acgtcaactt cacctacgtc tcgacgcagg
     4681 acggcaactg caacgcgcag aacacgcagg tgctgttcga cgtgaacccg gtcaacgcca
     4741 acggtcagta cctcgcacgc gcattcttcc cgaacagcac gcgcgccgaa cgcaacgtgc
     4801 tcatcgacac caccgcgttc cagccgggcg gcaccggcaa cgttccgctg cccaacatca
     4861 tctcgcacga gctcggtcac acgctcggct tccgccacga gcacatccgc cccgaagcaa
     4921 acgccacgca atgcgcggaa gacacccagt tccgcggcct cacgacgtac gacgcggcgt
     4981 cggtcatgca ctacccgcag tgcaacggca cgtcgcagga tctcgcgttc acgcagaagg
     5041 acaaggacgg cgtcgtcgcg ctgtacggcg caccggcgaa caacccgtcg ccgatgacgc
     5101 aagtcaccgc gcctgccgac aacgctaccg tcggccccaa cttcgacgtg gtcgcgtcga
     5161 tcatcgacac cgacctcatg aaggcggagc tcaagatcga cggcacgctg aaagacacgc
     5221 tcacggcagc gccgtacacg ttccacatca gcgcgctcgc agtcggcgct cacacgctcg
     5281 agatcacggc gaccgacggc gccggccaaa agacgacgca gacgctccac ctgacagttg
     5341 cgcaaaatgg tggcggcggc ggcaacggct cgggcggcgg cacgggcacc ggcggtggcg
     5401 acggcctcga cgattcgaac gtctacggcg gttgcgatgc gacgggcggc ggcggtgcgg
     5461 cgggcggcgg cctgctggca ctgatcgcat tcgcgagcct cgtgcgccgt cgcaggacag
     5521 tctaagctcg ctcgcatgaa gtggtggctc gcggcgctcg ccgtcgcgct cgcattcgag
     5581 aagccggcgg aaccgtgcgc gatcgcgccg cctgatacgt ctgcgcgcat ccagatcgtc
     5641 gacgaagaag cgctgatcat ctgggatccg gcgacgaaga ccgagcactt catccggcgc
     5701 gcgcagttcg cgtccgatgc gaaagcgttc ggcttcctcg tgccgacgcc gacgccgcca
     5761 aagctcacgg agctcgacgc cgaagtcttc gcgatcctcg ccgaccgcac ggcgccgcag
     5821 gtcgagtacc gcacggagcg gccgtggcag ctcggcgcgt ggctgctcga aggctgcatg
     5881 ctgatgaaag gcgacaaggc gatgcctgca ggcgcgccga tcgacagtgg cgtgcgcgtg
     5941 ctgtcgacga cgaacgtggc tggctacgac gccaccgcgc tgcaagcaag cgatccgagc
     6001 gcgctcggcg cgtggctcac ggcacacggc ttcgtgatga cgcccgcgat cgagcagtgg
     6061 ctcgccatct acgtgcagaa gcaatggacg atgacggcgt tcgtcgtcgc gggtgccgag
     6121 tcgacgcacg gtgacgtcac gacgcgcgcg atcgatatga cgtttcaaac cgacacgccg
     6181 ttctacccgt atcgcgagcc gggcgcgggc acggagctcg atggggtcac ctccgcgccg
     6241 ggccgctcgc tgcgcgtcta cttcgcagca ccgcagcggc acgccgcaac gctcgcgacg
     6301 aaaccgtggg ccgcaaaggt cagctatgcg cacgagttct ccggcgtccc gggcgtcgag
     6361 gcgctgctcg gcgcgagccg gtacatcacc gccttcatcg acgagacgtc gccgcggcgc
     6421 gggatcgacg agctctactt cgcgccctcc gtgacggggg atgtcgtgcc gccgccgttc
     6481 gtcgtgaccg accgggagcc gcgcctgatc cccgtcgaca tgatcctcat cgtcggcctg
     6541 ttcggcctct ggatcgtgcg ccggctccgc cgcattcgcc gtcgagcccg ggcctcgtaa
     6601 ctgcccacct acgtcgtcac tggctactcc agtagccggc gtccagccat cgttagctcc
     6661 cggaacaccg cgagacgacg agccttcaaa ctggtccggt ccttgtaaag ccatgggtta
     6721 tggccacgga atccacggcg atcgtgaacc tgctcgacct ggtcaaaaac cagaagcccc
     6781 tcgacagaga ctccgccgac gacatgctgt tcggcggtcg cgtccgcttc gatccgatgg
     6841 cgatgcccgc accggcgcag cccgtgatgc tccagccgat tccgtcgcta gcgcagggca
     6901 cgcccgtctc cgtgccactg ccgcagccgt tcgtgcgccc cgacccgaag gcgacgctcg
     6961 cgaagttcgc gcttcccgtc acgctcttct gctgcgtgat ggtgctgctc tctgtctacc
     7021 tctggaaagg cgagtcgaag tcgacgcccg cgcccaaggc aacgatcgtc cacgcgcctc
     7081 cgcccgcgcc cgtcgcgcac gtgatgcccg aagctccgcc gcagccaaca gtcaccgaaa
     7141 tccagccgac ggttccgacc gtccccgaga tgcagccgac cgttccgacg gtgccgaccg
     7201 ttcctaccgt cgcagctgca gacgaggcac aagacgacgc cgccgctgac gaggcaaagc
     7261 ccgtcgcgaa gtcagcgaag agcaagcgca gcaagcatca cagccgcgcc gcgaagcgct
     7321 cgaagaagga gacgcgcgtc gcggtggcgg aggctccgaa ggcaaagcca gagaagctcg
     7381 gcggcaaagg cgctcttcag ctctccagct cgcccgcgcg cgaagtctgg gtcgacggca
     7441 agaacacgaa gcagtcgact ccgcagaagc tgacgctcgc gccgggcacg cacaacatca
     7501 cgctgttcga caagaagacg cgcagcgcga agacgttcca ggtcgagatc aagccgaacg
     7561 agacgacccg cgtttcgaag aagtactaga gcgctgcggt ccaggcttcg gtcccggcat
     7621 catcgaagac gacggagcgg ccttcgcaaa accgcgctag caaccgctcg gctttcggat
     7681 cgacgatgaa gtccgttggc gctccgcgat gtcctccggg cggtggcaaa ccttcgacgg
     7741 tgatcacgag cagtccatcg acgcggtacg ccgagcgaaa cgagcggatc gggccgagct
     7801 cgaggatgct gcgcagcgcg atcgacgtcg ggtcgacgaa cgcgagagca gcgtggttcg
     7861 tgtctcccca ggttcgcttc agccacacga ctaacgagtc accgatcaga tcgatgtagt
     7921 cgtcggcctc ccagccgtcg atcgcgatgt cgccggttac cgcaggcgca gctcgctcgt
     7981 agcgcacgat gctgcggctc ggatcgtcgt cttcttggac gtacacgccg ccccacgcct
     8041 tgagcgtgtg cccgccgtac caaatggcgt cgccgtgcct gcccgacagc tcggtcgctt
     8101 tctcgacgag cgcatcgagc tcctcttcgg atcgcacggc tccgcgttcg cagacagtgc
     8161 catcagcggc ggtgacgcgg agccacgtac gatccgcgaa tgcgaggaag acctcgtcat
     8221 cgccgcgtcg catcggtgcg cgatgtagct tcgcgggatg cgcgagctcc cagcgcacgt
     8281 tgcccgtctc gagctcgcgc gcggccaccg cattcgttct cggaatgatg acgagatcgc
     8341 cgatccggcc gacgaagccg ttcgagtaga cgtcccggtc gacgagcttc tcgtcccagt
     8401 cgcgcagata gccgccgatg ccgagcgaaa cgcgacgcac gtagtgctcg tccatgttcg
     8461 gctcgcctgt gtagacaagc gaacggatcg gcagcagcgg atcgcgaccg cggtcgcacg
     8521 cgagcaggcg gctgaagtag ccccaaatcg caatcggcgg cggaccttca gacgatgtcg
     8581 tgtcggtgac ttcgacgaac gccgggtcgc gatacatgcc taccagacta cttgagcctg
     8641 cagcttgaag acgtttgcgt cgggcattgt ctcgagcggc acttggccgg ggcgcaggcg
     8701 gactttgtcg ccgtaccagt agtgcgagta catgaagaac agctcgcccc acgtatcgag
     8761 cgggaacgtg atgcgcggcg agaggacgcg gaagctgttt tcggagtcgt acatgtcgag
     8821 gatgacgcgg tcgaagcgcg cggacacgcg aagcttgggc aggacgagga acgacgggtc
     8881 gaggccccac ttgaggtaga ggcgccggtc gcgattgatc tgcggatcca tctcgtcgac
     8941 ttgcggcgag cgcacccacg tcgccatgcc gaacacgcgc agaccgactt ggtccgtgat
     9001 gttcgttttg acgtcggtcg cgagcgtgta catgtgcccg ctgccgtctt cggatgcgtc
     9061 gaggccgagg tagttttcgg tcaggccgcg gccgcccgtg gagtgcatga cctcgagcgc
     9121 gggcgcgagg tagagcacgt ggtccatgtg gtagtagccg aacgcgaggt acgcggtgcc
     9181 gcggtcttcg gggagcttgt agcgcgcgtc gagtccggcg acgtacatcg tgccgtcttg
     9241 gatcggcgag agctggcgct tgtcgcgcgt ccacgtgcga agcgtgtagg caccgagctc
     9301 gacggggccg accgggtagg cgcccgcaat gtgtgcgacc ggcgtcaggc cgaggttttg
     9361 ttggagctgc gcttggtgga tgccgatgcc ggtttggatg cggccaccgc ccggtagctc
     9421 gtaggcgacc tttgcgccgc cttgatgcgt gcggccgtag agatacgtgt cgtacggctc
     9481 gatgtagccg aagcgatccc agaagacgcc gacttgaacc gagagcgggt cgacgccggc
     9541 gaagtgcttg gcgctcaccg aggcttgcgc gacgccgagc tggttctcga ggcgcgcggt
     9601 cgcgtagtcg gagtagagcg aggcgaacag gccgaacttg gcttcgtagt caccgtgctt
     9661 ggcgctgaag aacagctcgg accaatcggg ctcgtagagg cgcgtgtacg cgaagcccga
     9721 gaggtagtag tcgttgtcga cgagatatgg ctcgcgcggc gtgccttgag cagcgagcgg
     9781 catgcgtgcg tagccgtgca gctcgaagcg cgtctcgccg acttcgacgg ctttcgtcgt
     9841 gccttgcggc gcgtctttcg agatggagac ggactcggtc gtcgtcggct gctcgatcgc
     9901 ggcggttttg acttcttcct tcttctcttc ctgcggcttg gtctcttccg taggcgcttg
     9961 ctcgtcggcc gatgcgaccg acgtcaggag taggaacgct gcgatcgtcg tcttcatgct
    10021 agtgcaccgc ggcgaggcgc gatttgcagg cggggagctc gtcggccgag aggaagcccg
    10081 tgtcgacgac gagcgccttc acggtgtcgg gcgtgacgac ttcgacgcgc acggccgcga
    10141 ccgggatgtt gccgcctgcg agcgcgatcg aggagcccgc cgctggcgtg gtcccgtcga
    10201 gcagttgttt cgagacgtca gctgcggtct tcgcgagcgg ctggatgtcc ttgaggactt
    10261 cgatcgcttg cttgccttgg cagacgaagt tgacgttcgc cgcatcggca tcggcgccgg
    10321 cgatgaacac gtgatcgagg cccgccgcgt tcactgcttg gaccgcgccg cgcgccatgc
    10381 ccgagttgtt cgcgaggatc gcgtcaacgt ggttgctgct gcgcgtgagc gcgtcttcga
    10441 cggtgcgcag cgcctgctcg ggggaccacg acgagtggtt ctgctcgagg acgatttcga
    10501 tatcaccttt ggcgacgtac ggcgcgagcg tgtctttgta gccgcgcgtg atctccgtcg
    10561 cgaccgagtg accggcttgg cccgagagga tgacgtactt gcccttgttg ccggtcgcgg
    10621 cgagggcggc ttgcgcctgg agcacgccga cgcggtagct atcgtgcgag acgtagtagt
    10681 cgagatcggg ggcgacgatc gcgcggtcgt acgcgatgat ctttgcgccc ttctcgtgcg
    10741 ccagcttcac gtagctcgac gccgcttgcg aatcggtcgg ctggatcacg aggaccttca
    10801 caccttgcgt gagcacgtct tcgacttgag caatctgctt cgcgttgtcg ttgtcagccg
    10861 caagcgtgac gacgcggaag ccgaggtcct tcgcgcgcgc ttcgaagtac ttgacgtctt
    10921 tctggtagcg ctcttcttga agtg
//
LOCUS       DATLBZ010000427         4958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2760574, whole genome shotgun sequence.
ACCESSION   DATLBZ010000427 DATLBZ010000000
VERSION     DATLBZ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2760574"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VMZ53_21670"
     CDS             <1..617
                     /locus_tag="VMZ53_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HUS31140.1"
                     /translation="SNYSGQIGKKVASDLCTVVDDGTIQGSRGAINVDDEGYAGQNNV
                     LIEKGVLVGYMHDRLSAKHFGIEPTGNGRRESFRAMPLPRMTNTSLLAGPHTHDEIIK
                     SVKKGIYAKRFSGGQVNISNGDFVFSLTESYLIEDGKLTAPLKGVNLIGNGPDVLRRV
                     DMLGNDYELSDGIWTCGKDGQSVPVNVGTPTVRVASITVGGTQV"
     gene            614..2014
                     /locus_tag="VMZ53_21675"
     CDS             614..2014
                     /locus_tag="VMZ53_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HUS31141.1"
                     /translation="MSSGVQVTEKTIKELCDAAQLAVELAKKAGADDAEVLVRDGSEL
                     TAKVRLGEPELVQEATSRGLGLRVLKGGRRAVTYTSDLRREALEALCAETVALAELAE
                     PDEYALPPDPALLAKQIPELDLYDDKVAEVEAAWALREAKNGEAAARRSDARVTNSEG
                     ATWSRVLGATAFATSGGFVGGYRGSYASLVVEPLCDDMTDPENPKKRNGYWWTGSRFL
                     DQLEVAEAVGIEATRRTVATIGSKKVDTQECPIVFDPEIARSIVGTIFSVANGSAFWR
                     KSSYMVGKEGQEIASKLVTIIDDPLIPRAPGSRPFDGDGLATRKNMVVTAGVLAPVLC
                     DVYSGRKLGRASTGSAGRGIGGNPGPTTSNLIMEAGSITREELLKQTDRGLYVTSLMG
                     FGFNPVTGDFSRGAQGFWIEKGELTFPVSEVTIAANFDQILKRIDLVANDLVLRSSTA
                     APTFRVSHMMLAGTSR"
     gene            2100..4667
                     /locus_tag="VMZ53_21680"
     CDS             2100..4667
                     /locus_tag="VMZ53_21680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31142.1"
                     /translation="MPAAATAAGLPSRLGKYELVMPLAAGGMARIYIGRSTGIGSFER
                     HVVLKMITPERANDQTTINMFLDEARLAASLNHQNVAQVFEVGEDLGVHYLAMEYVHG
                     QDLRAVLAKAGSGGTRIPLELALTVVAGAAAGLHHAHERRGPDGVPLGIVHRDVSPSN
                     IMIGYDGAVKLLDFGIAKATSRSVETQSGIIKGKFAYMAPEQCRGRDVDRRSDVFSLG
                     IILYEVSTQHRCFRADSDFDTMHRIVTGDVVRPTRLVQGYPQALEAIVMKALATDAAQ
                     RYQSSGALLEALEQFAVASRMSLSTMGLGRFMRDMFGEVQEPWLNTAATKVLQPLAAK
                     ESTISSTNGKGAAAQRIEAPEHLDPDDDLDGVPDEDGSFPDSAGVAMQTVLDLPKGGG
                     RSETEPPADWNAKSYPSTGAPNDMRGYPSTTPMPMGQQSSAQHLHQPIGQMPLPAPPH
                     PGTPPVGISQMQQAPLGTQPMGTVPVGSRSDMTPVPQGGAFQTPGSGTGPAMAHPGGG
                     PIVSTKHGYASAAMSRGDVSYGNYTMPVDAYSDVQLKPNRRPLFIGLGLAIVGIIVIL
                     AVSFGGKEEKAASPTPDDDGSGDGSADTTETMPTPQKGSDSAPGPLDENMVSIIIDSE
                     PHGADVLIAGTKIGTTPFNKKLKRGTSRAELKVRLTGYVDFTAKLDLAGDYENPSIKL
                     TKVEDAKGSADPSTSGGDKATGNGDKATGDGDQGAGDTGTSGASTTKTGTTSPGTSST
                     GATDKTGTSTTNTATADKTGTKTGTTGTTTKTGTGTTSATSSTGTKTGTTTSSTGTKT
                     GTTTSATGTKTGTGTGTTSTGTGTGTKAGTGTGAKVKCQTAGPNVDPFSNVPICPAK"
BASE COUNT          984 a         1609 c         1624 g          741 t
ORIGIN      
        1 cgagcaacta ctcgggccag atcggcaaga aggtcgcgag tgacctctgc accgtcgtcg
       61 atgacggcac gatccagggc tcgcgcggtg cgatcaacgt cgatgacgaa ggctacgccg
      121 gccagaacaa cgtgctgatc gagaagggcg tgctcgtcgg ctatatgcat gaccggctct
      181 ccgcgaagca cttcggcatc gagccgacgg gcaatggccg ccgcgagtcg ttccgcgcga
      241 tgccgctgcc tcgcatgacg aacacgtcgc tgctcgctgg gccgcacacg cacgacgaga
      301 tcatcaagag cgtgaagaag ggcatctacg cgaagcgctt cagcggcggg caagtgaaca
      361 tctccaacgg cgacttcgtg ttctcgctga ccgagagcta cttgatcgaa gacgggaagc
      421 tcaccgcgcc gctgaagggc gtgaacctga tcggcaacgg tccggacgtg cttcgccgcg
      481 tcgacatgct cggcaacgac tacgagctgt ccgacggcat ctggacgtgt ggcaaggacg
      541 gtcagtccgt gcccgtcaac gtaggcacac cgacggtccg cgtcgcgtcg atcacggtcg
      601 gaggcacgca ggtatgagca gcggcgttca agtcacagag aagacgatca aggagctgtg
      661 cgatgcagcg cagctcgcgg tcgagctcgc gaagaaggcc ggcgccgacg atgcagaagt
      721 gctcgttcgc gatggcagcg agctgactgc gaaggttcgc ctcggcgagc cggagctcgt
      781 gcaagaagcg acgagtcgtg gcctcggtct gcgcgtgctc aaaggcggac gccgcgcggt
      841 gacgtacacg agcgacttgc gccgcgaagc actcgaagcg ctgtgcgcgg agaccgtcgc
      901 gcttgccgag ctcgccgagc ccgacgagta cgcattgccg ccggatcctg cgttgctcgc
      961 aaagcagatt cccgagctcg atctctacga cgacaaagtc gctgaggtcg aagcggcgtg
     1021 ggcattgcgc gaagcgaaga acggtgaagc cgcagcgcgc cgcagtgatg cacgcgtgac
     1081 gaacagcgaa ggcgcgacct ggtctcgcgt gctcggtgcg acggcgttcg cgacgagcgg
     1141 cggttttgtc ggcggctatc gcggcagtta cgcatcgctt gtcgtcgagc cgctgtgcga
     1201 cgacatgacg gatccggaga atccgaaaaa gcgaaacggc tactggtgga ctggctcgcg
     1261 cttcctcgat cagctcgagg tggcggaagc cgtcggcatc gaagcaacgc ggcgcacggt
     1321 cgcgacgatc ggatcgaaga aggtcgacac gcaggagtgt ccgatcgtgt tcgatccgga
     1381 aatcgctcgc tcgatcgtcg gcacgatctt cagcgtcgcg aacggctctg cgttctggcg
     1441 caagtcgagc tacatggtcg ggaaggaagg gcaggagatc gcgtcgaagc tcgtcacgat
     1501 catcgacgac ccgctcatcc cgcgcgcgcc cggctcgcgc ccgttcgacg gcgacggtct
     1561 cgcgacgcgc aagaacatgg tggtcaccgc cggtgtgctc gcacccgtac tctgcgacgt
     1621 ctactcgggc cgcaagctcg gtcgcgcatc gacgggctcg gcaggacgcg gcatcggtgg
     1681 caaccccggt ccgaccacga gcaacctgat catggaagca ggtagcatca cccgtgagga
     1741 gctactgaag cagaccgacc gcggcctgta cgtcacctcc ctcatgggct ttggcttcaa
     1801 cccggtgact ggggatttct cgcgtggcgc gcaggggttc tggatcgaga agggcgagct
     1861 gacgttcccg gtctccgaag tgacgatcgc tgcgaacttc gaccagatct tgaagcgcat
     1921 cgacctcgtc gccaacgacc tcgtcctacg tagctccaca gctgctccaa cgttccgcgt
     1981 gtcgcatatg atgctcgcgg gcacgtctcg gtagtgcacc tacgtacctg agatcgttgg
     2041 aggtcctaag gtcggtttgc ttgacttgaa ccacctggaa tgaaacgatc ttcggagtaa
     2101 tgccagccgc cgcaacggcc gcgggactac cgtcccgcct cggcaagtac gagttggtga
     2161 tgccactcgc ggccggtggc atggcgcgaa tctacatcgg ccggagcacc ggcattggtt
     2221 cgttcgagcg ccacgtcgtt ctgaaaatga tcacgccaga gcgcgcgaat gatcagacga
     2281 cgatcaacat gttcctcgat gaagcgcgac tcgctgcttc gttgaaccat cagaacgtcg
     2341 cgcaggtgtt cgaggttggc gaggacctag gcgtccacta cctcgcgatg gagtacgtgc
     2401 acggacagga tcttcgtgcg gtgctcgcga aagctggaag cggcggtaca cggattccgc
     2461 tcgagctcgc gctcaccgtc gtcgcaggcg cggctgcagg tctgcaccac gcacacgagc
     2521 gtcgcggtcc tgacggcgtc ccgctcggca tcgtgcatcg cgacgtttcg ccgtcgaaca
     2581 tcatgatcgg ctacgacggc gccgtgaagc tgctcgactt cggcatcgcg aaagcgacgt
     2641 cgcgttccgt cgagacgcag tccggaatca tcaagggcaa gttcgcctat atggcgcccg
     2701 agcaatgccg cggccgcgac gtcgatcgcc gcagcgacgt gttctcgctc ggcatcatcc
     2761 tctacgaagt ctcgacgcag catcgctgct tccgcgccga cagcgacttc gacacgatgc
     2821 accgcatcgt caccggcgac gtcgtgcgtc cgacgcgact cgttcaaggc tacccacaag
     2881 cgctcgaagc gatcgtcatg aaggcgctcg cgaccgacgc ggcacagcgc taccaatcgt
     2941 cgggcgcgct gctcgaagcc ctcgagcaat tcgcggtcgc aagccgcatg tcgttgtcta
     3001 cgatgggcct cggccgtttc atgcgcgaca tgttcggcga ggtgcaggag ccgtggctca
     3061 acaccgccgc gacgaaagtg ctgcagccac tcgccgcgaa ggaatcgacg atctcgtcga
     3121 cgaacggcaa gggcgcggcc gcgcaacgaa tcgaagcgcc cgagcacctc gatcccgacg
     3181 acgatctcga cggcgttccc gacgaggacg gctcgttccc agacagcgct ggcgtcgcga
     3241 tgcagaccgt cctcgatctg ccgaagggtg gcggtcgctc ggagaccgag ccgccggcgg
     3301 actggaacgc gaagtcgtat ccgtcgacgg gcgcgccgaa cgacatgcgc ggctatccga
     3361 gcaccacgcc gatgccgatg ggccagcagt cgtccgctca gcatctgcat caaccgatcg
     3421 gccagatgcc gctgcctgcg ccgccgcatc cgggcacgcc gcccgtgggc atctcgcaaa
     3481 tgcagcaagc gccgctcggc acgcagccga tgggcaccgt tccggttggc tcgcgttcgg
     3541 acatgacgcc ggtgccgcag ggcggtgcgt tccagacgcc gggctcgggt accggacctg
     3601 cgatggcgca cccgggcggt ggaccgatcg tcagcacgaa gcacggctac gcatcggcgg
     3661 cgatgagccg cggcgacgtc tcgtacggca actacacgat gccggtcgac gcgtacagcg
     3721 acgtccagct caagccgaac cgccgcccgc tcttcatcgg cctcggcctc gcgatcgtcg
     3781 gcatcatcgt catcctcgcc gtatcgttcg gtggcaagga agagaaggcc gcctcgccaa
     3841 cgcccgacga cgacggctcc ggcgacggct cagcagacac gaccgaaacg atgccgacgc
     3901 cgcagaaggg ctccgactcg gcacccggac cgctcgacga gaacatggtg tcgatcatca
     3961 tcgactccga gccgcacggc gccgacgtcc tgatcgccgg cacgaagatc ggcacgacgc
     4021 cattcaacaa gaagctcaaa cgcggtacga gccgtgcgga gctgaaggtc aggctgaccg
     4081 gctacgtcga cttcacggcg aagctcgatc tcgcgggcga ctacgagaac ccgagcatca
     4141 agctgacgaa ggtcgaagac gcaaagggca gcgccgatcc gtcgacgagc ggcggcgaca
     4201 aggccacggg caacggcgac aaggcgactg gcgatggcga ccaaggcgcg ggcgacaccg
     4261 gcacctccgg cgcgtccacg accaagaccg gcacgacctc gccgggaacg tcctcgaccg
     4321 gcgcgacaga caagaccggc acctcgacga ccaacaccgc gacagcggac aagaccggca
     4381 ccaagaccgg cacgaccggc accaccacga agaccggaac cggaacgacg tcggcgacat
     4441 cctcgacggg tacgaagacc ggcacgacga cttcgtcgac cggtacgaag accggcacga
     4501 cgacttcggc gacgggcacg aagacgggga cgggcacggg aacgaccagc acgggtacgg
     4561 gcacgggcac gaaggccggc accgggaccg gcgcgaaggt gaagtgtcag acggcgggtc
     4621 cgaacgttga tccgttcagc aacgttccga tctgtcccgc caagtagcgc gtccgaatca
     4681 cggacaccgg ctaagggacg gacgcaactt ccgcaacccg cggacgtagt gaagatcgcg
     4741 atcgttgagt gactaaggga cggacgcaac ttccgcaacc cgcggacgta gtgaagatcg
     4801 atcgttgcac gcggtgccga aacgcgattt cgcgaaccgg ggccgcctgc aagatacaat
     4861 cggcgattgc aggcaggttg cggaagttgc gtccgtccct tattgcgagc tagaggcgca
     4921 tctcgggcac gtcgccgctc acgatgagcg gtgcgccg
//
LOCUS       DATLBZ010000428         4576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5741600, whole genome shotgun sequence.
ACCESSION   DATLBZ010000428 DATLBZ010000000
VERSION     DATLBZ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5741600"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VMZ53_21685"
     CDS             complement(<1..542)
                     /locus_tag="VMZ53_21685"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HUS31143.1"
                     /translation="MNSLLPTTPAGASAGAELFTSDGRALPLIGARLIGEAQGGLARL
                     VLEQTFANTYAENLKVTYRMPLPADGAVSGYEFVIGERVVKGAVDKKQRARERFEQAI
                     ASGRTAALLEQERADIFTQQIGNIPPGEAVVARITIDQRLVWLPEGEWELRFPTVIGP
                     RYIGSVDTAADIRATHIKVT"
     gene            complement(656..1096)
                     /locus_tag="VMZ53_21690"
     CDS             complement(656..1096)
                     /locus_tag="VMZ53_21690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31144.1"
                     /translation="MGIVLMLVACTHAAPAPVATSRPAQPTTQQPPAKKIKIVTDTRV
                     EILDPIKFVAGSPSIEPASIPIIDAIAGTLIGNPSIKLVEVIAFGNDAVPSLRESVAV
                     ARAQAVVGELIARGVDSRRLRANGEAVPRQGLGPEPMFLILERD"
     gene            complement(1138..2727)
                     /locus_tag="VMZ53_21695"
     CDS             complement(1138..2727)
                     /locus_tag="VMZ53_21695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31145.1"
                     /translation="MRSGTSGLANAADALRLEEVKRTRALLQLSWIIALGLTGAILIA
                     PGNPRIARALIAVLGVGVVGSFWLYRELANPARYSASRMNVLALLAVVAGQLGILYVG
                     AFSAAPIMVSLGLYFFCRTESLRSAIAIYVLAAGAHAVTAALVVSRVLDDPGFYPIGD
                     RASVQAQIAGQGLMQFAYALCFWLARVTRKTSLKSIEHLQKATRLAAQRDVQLNELRH
                     DLDRALKVGGPGRFTGVVVGTWELGVVLGRGAMGEVYAATSTQTEGQQSAVKLLRREL
                     LADPRHVERFLREVRIASSIDSRHVVRVLEASTPSDPVPFLAMEKLEGQTLASLLRKG
                     RSLSDSSLEALVAQVCDVLELARAAGIVHRDIKPQNLFLTFDDVWKVLDFGVALLSDS
                     SGTLTQGAAVGTPAYMSPEQARGAPVDHRADVYAMGAVIYRCVTGRVPFARPDTPSLL
                     FAVVQEMPVRPSAIAKSSPQLDAVLQIALAKDRDARFATARDLADAFTAASRNDLPYS
                     TLHRAKVLQPWAEPPPVTPTP"
     gene            complement(2757..4478)
                     /locus_tag="VMZ53_21700"
     CDS             complement(2757..4478)
                     /locus_tag="VMZ53_21700"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31146.1"
                     /translation="MPGEPTDDGATLIDPQTGPTAPLTQVPAPSPMALVAAGRAPSQP
                     SSQPSGASRSVSLTTAADAMRNEEVERTRLFIMMGWVISVITGGAIFYIDAPMWIAYV
                     FVGGLAVGMVLSTYYYRAFADPLRYTETALVRLAMICVFNGHAGVLLFGTYSAAPILT
                     VVGIHFVARTELERVARWVLISALVFYTAISLVLISGIVDDPGVFDSNRPVPKLSLVA
                     ATLFVLGTYVLAYYTARSFRSASLISIDELQRTTRLASQREALMDELRADLERALRVG
                     GPGRYTDQVVGTFKLGIVLGRGAMGEVYEAAHVTSGAAAAVKLLRREQLSDPTQVARF
                     LREVKASAAIDSPHIVRVLDAAAADDQLPYLAMERLHGQTLSELLRREPKLSASQTVE
                     LCRHTAIGIDALAGVGIVHRDLKPANLFQTADGVWKIIDFGVATLGDGSGTLTQGEAV
                     GTPYYMAPEQAQGLKVDSRADVYALAAIAYRCLTGRYPFTGTDTPALLYAVVHRTPPR
                     PGELVELPEDIDRWFAIALAKQPGDRFATGADAVDALIAAFGHKLDAKLRKRADALIR
                     KHPWEAV"
BASE COUNT          786 a         1481 c         1575 g          734 t
ORIGIN      
        1 tccgtcacct tgatgtgcgt cgcgcgaatg tccgcagccg tgtcgacgct gccgatgtag
       61 cgcgggccga tcacggtcgg aaaccgcagc tcccattcgc cttcgggcag ccataccagc
      121 cgctgatcga tcgtgatgcg agcgacgacc gcttcgcccg gcgggatgtt gccgatctgc
      181 tgcgtgaaga tgtctgcgcg ctcctgctcc agcaatgcgg ccgtacgacc cgatgcgatc
      241 gcctgctcga accgctcacg cgcgcgctgc ttcttgtcga cggcgccttt cacgacgcgc
      301 tcgccgatca cgaactcgta gccactcact gcgccatcgg caggcagcgg catgcggtac
      361 gtgaccttga gattctcggc gtaggtgttc gcgaacgtct gctcgagcac cagtcgcgcg
      421 agtccgccct gcgcttcacc gatgagacgt gcgccgataa ggggcagcgc acggccgtcg
      481 ctcgtgaaga gctcggcgcc ggcagaggca ccggcagggg tggtagggag gagcgaattc
      541 acggcgcgcg acgatgacga aggtagctgg agcagcatgg tggttgggtc ctttcacccg
      601 accaacgcga cagccctcgc tccagtgaca aagaatcgta cgagcgtgta acttgctagt
      661 cccgctcgag aatcaggaac atcggctccg gtcccaagcc ctgtcgcggc accgcttcgc
      721 cgttggcccg aagccgccgg ctgtcgacgc cgcgtgcgat cagctcgccg accacggcct
      781 gcgcgcgcgc cactgcgacc gactcgcgca gcgacggcac cgcatcgttg ccgaacgcga
      841 tcacttcgac gagcttgatg ctcggattgc cgatcaacgt gcctgcgatc gcgtcgatga
      901 tcggaatgct ggcgggctcg atcgacggag aaccggcgac gaacttgatc ggatcgagga
      961 tctcgacgcg cgtgtcggtg acgatcttga tcttctttgc gggtggctgc tgcgttgtcg
     1021 gttgcgcggg ccggctcgtc gcaacgggcg ccggcgccgc gtgcgtgcac gcgactagca
     1081 tgagcacgat ccccactcgc cacattgttg ttaactgtgc catggggccc gtgtggatca
     1141 aggcgtcggc gtgaccggcg gcggctcggc ccacggctgc agcaccttgg cgcggtgcaa
     1201 ggtcgagtac ggcaggtcgt ttcgcgaagc ggcggtgaag gcgtcggcga ggtcgcgcgc
     1261 cgttgcgaag cgggcgtcgc ggtctttcgc gagcgcgatc tgcagcaccg catcgagctg
     1321 cggcgacgac ttcgcgatcg ctgacgggcg tacgggcatc tcttgcacga cggcgaacag
     1381 cagcgatggc gtgtccggcc gcgcgaacgg cacgcgaccc gtgacgcagc gatagatgac
     1441 ggcgcccatc gcgtagacgt ccgcccgatg atcgaccggc gcaccgcgcg cttgctcggg
     1501 agacatatag gccggcgtgc cgaccgcggc gccttgcgtg agcgtgcccg acgagtcgga
     1561 gagcagggcc acgccgaagt cgagcacctt ccagacgtcg tcgaatgtga ggaagagatt
     1621 ctgcggcttg atatcgcgat gcacgatacc tgcggcgcgc gcgagctcga gcacatcgca
     1681 gacctgcgcg acgagcgctt cgagcgagct gtcgctgagc gagcggcctt tgcggaggag
     1741 cgacgcgagc gtctggcctt cgagcttctc catcgcgagg aacggaactg gatcgctcgg
     1801 cgtggatgct tcgagcacgc gcacgacgtg acgcgaatcg atcgagctcg cgatccggac
     1861 ttcgcggaga aagcgttcga cgtgacgcgg atcggcgagc agctcgcggc gcaggagctt
     1921 cacggcggat tgttggcctt cggtctgcgt gctcgtcgct gcgtagacct cgcccatcgc
     1981 gccgcggccg aggacgacgc cgagctccca cgtgccgacg acgacgcccg tgaagcggcc
     2041 gggcccgccg actttgagcg cgcgatcgag atcgtgacgc agctcgttga gctgaacgtc
     2101 ccgctgcgcg gcgaggcgcg tcgccttctg cagatgctcg atcgacttca gcgatgtctt
     2161 gcgcgtgacg cgcgcgagcc agaagcacag ggcgtaggcg aactgcatga ggccttggcc
     2221 tgcgatctgc gcttgcaccg acgctcgatc gccgattgga tagaagcccg gatcatcgag
     2281 cacgcgtgag acaacgagcg cagcagtgac ggcgtgtgca ccagccgcga gcacgtagat
     2341 cgcgatcgcg ctgcgcaggc tctccgtgcg gcagaagaag tagaggccga gcgacaccat
     2401 gatcggcgcc gccgagaacg caccgacgta gaggatgccg agctggccag cgacgacagc
     2461 gagcagcgcg agcacgttca tgcgtgacgc cgagtagcgc gccgggtttg cgagctcgcg
     2521 gtacagccag aagctgccaa cgacgccgac gccgaggacg gcgatgagtg cgcgcgcgat
     2581 gcgcggattg cccggcgcga tcaagatggc gcctgtgaga cctagcgcga tgatccagct
     2641 cagttggagc agtgcacgcg tgcgttttac ctcttcgagc cgaagcgcat cggccgcgtt
     2701 cgcgagaccg cttgtcccgc tccgcactga caacgacgtc tgggacgttc gcgtggtcac
     2761 acggcctccc acggatgctt gcggatcaac gcgtcggccc gcttgcggag ctttgcgtcg
     2821 agcttgtggc cgaaggcggc gatcagcgca tcgaccgcgt cggcccccgt cgcgaatcgg
     2881 tctccgggct gcttcgcgag cgcgatcgca aaccagcgat cgatgtcttc cggaagctcg
     2941 acgagctcgc caggccgcgg tggcgtgcga tgcacgacgg catagaggag cgccggcgtg
     3001 tcggtgcccg tgaatggata ccgaccggtg aggcagcgat atgcgatcgc ggcgagcgcg
     3061 tagacatccg cgcgcgagtc gaccttcagc ccctgcgctt gctcgggcgc catgtagtaa
     3121 ggcgtgccga cggcctcgcc ttgcgtgagc gtgccgctgc catcgccgag cgtcgcgacg
     3181 ccgaaatcga tgatcttcca cacgccgtcg gcggtctgga acaagttcgc cggcttgaga
     3241 tcgcgatgga cgatgcccac gccggcgagc gcatcgatgc cgatcgcggt gtgcctgcac
     3301 agctcgacgg tctggctcgc cgatagcttc ggctctcgac gcagcagctc gctgagcgtc
     3361 tggccgtgga gccgctccat cgcgagatac ggcagctggt cgtcggccgc agctgcgtcg
     3421 agcacgcgca cgatgtgtgg cgaatcgatc gcggcgcttg ccttcacttc gcgcaagaag
     3481 cgcgcgactt gcgttggatc cgacagctgc tcgcggcgca acagcttcac cgcagccgct
     3541 gcaccgctcg tgacgtgtgc cgcttcgtag acctcgccca tcgcaccgcg cccgaggacg
     3601 ataccgagct tgaacgtgcc gacgacctgg tccgtgtacc ggcccgggcc gccgactcgc
     3661 agtgcgcgct cgagatcggc gcggagctcg tccatgagtg cctcgcgttg cgatgcgagc
     3721 cgcgtcgtgc gctggagctc gtcgatcgag atgagcgacg ccgaccgaaa gctgcgcgcg
     3781 gtgtaatacg cgagcacgta cgtaccgagg acgaacaacg tcgccgcaac gagcgagagc
     3841 tttggcaccg gccggttcga gtcgaagacg ccggggtcgt cgacgatgcc gctgatcagc
     3901 accagcgaga tcgccgtgta aaagacgagc gcggagatca gcacccagcg ggcgacgcgt
     3961 tcgagctcgg tgcgcgcgac gaagtgaatg ccgacgaccg tgaggatcgg cgccgcggag
     4021 tacgtgccga acaggagcac gccggcatga ccgttgaaca cgcagatcat tgcgagtcga
     4081 acgagtgcgg tctccgtgta acgcagcggg tccgcgaacg cgcggtagta ataggtgctc
     4141 agcaccatgc cgacagcgag gccgccgacg aacacgtagg cgatccacat cggcgcatcg
     4201 atatagaaga tcgcgccgcc cgtgatgacg ctgatcaccc agcccatcat gatgaagagc
     4261 cgcgtgcgct cgacctcttc gttgcgcatc gcatcggcgg ccgtggtcag cgagacggac
     4321 ctcgatgctc ccgaaggctg cgacgatggc tgggagggcg cgcgaccagc ggccaccagg
     4381 gccatcggag acggcgcggg cacttgggtc agaggcgccg tcggccctgt ttgcgggtcg
     4441 atcagcgtgg cgccatcgtc cgttggctca ccaggcactt ccgcgaagat aacagtgacc
     4501 tccgccacct ggacagaatt ggtcaggcgc gccggtccga gatcgggcta aaatcgctcc
     4561 gtgattcgcc tggggt
//
LOCUS       DATLBZ010000429        30900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4229193, whole genome shotgun sequence.
ACCESSION   DATLBZ010000429 DATLBZ010000000
VERSION     DATLBZ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 30900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30900
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4229193"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..573)
                     /locus_tag="VMZ53_21705"
     CDS             complement(250..573)
                     /locus_tag="VMZ53_21705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUS31147.1"
                     /translation="MRFLAYDLALEIITSLHASLAIIRRHNASLAKQGANAIDSVLLN
                     LAEGNGREGGDRLHHFRIALGSLREVGAVLDIAAAHRWLDSPPLAAERDRLCGMIYGL
                     QRSRR"
     gene            complement(821..3589)
                     /locus_tag="VMZ53_21710"
     CDS             complement(821..3589)
                     /locus_tag="VMZ53_21710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31148.1"
                     /translation="MSDAQAGSTASSYEILAKLSQGSGTAELFAARATGVDRLVVIKR
                     LRRDAAKEKAVAQAFLAEARVAAKLAHPNVAQVYEVGKLGGSYFFTTEYVDGETIRAL
                     IAHARGKKIQVPIRAFLTIAAGAAAGLHHAHERNGMDGKSLNIIHGDISATNVMISRE
                     GIVKVLDFGMARGRGPAKLVYASPEQVRGDKLDRRSDLFSLGVVLWELLTLGDLHDRP
                     TDEETRQAIENDDTPAPSLRRFDVPPELDQILVKLLAKKPDDRFQTGGELLAAIESLS
                     TKLGFSLSTNDLARMMRLWFGPPTSATEADDLKPIQVTSEDILSETSATTADPIDELL
                     ASVREAAAKIRSLAQRIGGSSRGDRPATSPPTAGEVENAREDFAQIRDRILEQARKRK
                     ETMRPPVSDQIAAVGATPPSGIATASANATPPSGVPAAVASPADTGSVRKRPETLTPT
                     NQYSYITRVAETASAPTVKATAAAADASAALKALEAASAHAEAASTATATALDSNGAN
                     GSNGAVVVGNRAVTATYDAAAVAAAGEVAVASQTAATSGEIGSKPASSDGPSTSDAKV
                     EIAAEPKSDAAAEAKADAKAEAKAETKADKPADKSAKKSDDDKPAVSRTASKSNGVSA
                     KASVKDDDDDDTDAAAASRRAAARTAVDADDSPSRRPGWLVPALLVAAVALILIFVLK
                     GRDGNDKPTKPDQVAQNQGDKTNTNTNTNTNTANTKTQEAGSAAAGSQTTTTTMQTPP
                     SVDAAVAETPIDAAVVAETPPVDAAVAVVVAPPVDAAVVAQNDKTNTGDKTNTGNKTN
                     TGDKTNTGDKTNTGNKTNTGDKTNTGNKTEPAKELSIEELYAKGDFATTNKACTKNLI
                     FSPAILQACALSACRSNNTALAKRWVNAIAKSARPDVVAQCKGFGLDLEAPPPGDAGS
                     AAAPAPAP"
     gene            3727..5190
                     /locus_tag="VMZ53_21715"
     CDS             3727..5190
                     /locus_tag="VMZ53_21715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31149.1"
                     /translation="MNVATRITLATAVVVALASAAYAAFDLRARRAERLEQLGREAKA
                     VAGAVRFQLEGQGSAFTAPSDKWLRDLEIKSGWNIKVAPRVRKDLPAGPDLSERELGW
                     LRALVESPTFQLTDNEIGDRYYYVLPLRAPALPSEEPTILGMLELNKSVAVTSSDDLG
                     RAVALVVLIVLVTTLMVGALASRFVSRPITKLLRGIDDVAKGDLSHVILAERDDEIGQ
                     IATRFNEMTFSLRESRGETQRQNEAKLALEQRLGQTEKLATIGQLAAEIAHEVGTPLN
                     VIAGRAKSMKRKSKDPEAVEKNAEIVAEQTARITRIIQRLLDFARRKVGTTGKAEINL
                     NELSLTTMEMLAGQFAAAKVKTKLDRAEGLPKVAGDADRLQQVLINLLLNAVQAMPDG
                     GSLIVETRAVKRTRPGLESGPEQSFVGIAVRDTGIGIPEDIREKIFDPFYTTKEGQGG
                     TGLGLAVCSGIVKEHDGWIDVEANDNGGTVFRVYLPA"
     gene            5247..6137
                     /locus_tag="VMZ53_21720"
     CDS             5247..6137
                     /locus_tag="VMZ53_21720"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS31150.1"
                     /translation="MQRVHLEYLGDSVELPVGETVIGRDLSCALRFNDAAVSRRHLRF
                     IRRQNEVFLEDLGSSNGTLLNGRNITGALRITDGDEIRVGSRLLTIRIVEGEEEPAAS
                     TLILKNFNAPEELRKLRATTSQVAVTQPPPMNANQRCPRCAAAVNIEDDECTNCKFRW
                     ADFRPQSRTDVRPNPLNRRRHDRKPIELSLVYSSSELEIEATSRDLSESGVFVCSQVL
                     DPIGTECQLTILVDGGPPIGVRGVVRRVVERETLDGEPIGIGVEFVKTGEAELSWIRT
                     VVSRMMQEVEDDETQPVDVR"
     gene            complement(6138..8489)
                     /locus_tag="VMZ53_21725"
     CDS             complement(6138..8489)
                     /locus_tag="VMZ53_21725"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS31151.1"
                     /translation="MTTLTRWKLACALFAALAGGAVVHAHKGGNHNVPAKQAAAPRGA
                     MPQVFRKPIHVSPEALGISQKDLIDRILAAKSVKDIALLTDKLGVVGDDDAVETLAPL
                     VEDPRHGVPEAMLSAFGQIGTQKAIDVIVAHTKDERPTVRSSAIYALGATHSEAAEKT
                     LVELSRAQGDPAQTTAISALGTLGTDNAVARLIELANMSDYQVSSTAVYALGSAGTPA
                     AETALRKLMDSADTRVAAAAIASIDTVDAPMLAKLTAIVKSGDPQLVNAALAALGHAG
                     EEGLPALKEAALHGSGNVRWTAVSAIGDVGGEKATQILVEILKSGDRQSASAAVQALA
                     TIGGQDARDALIEAALSDRANMTGALYQLAQMQGEDVEQALISVVKQGSSQERRAVLP
                     RLVRAGNQEAIQLAIDLAQKGSRNERYDAMRVLGDAGTPKAFDALMDIASKSHGSSRV
                     QALEYLATSHPADPAVGQMLSDALFSGRREEANYAASVLGRVGTEDARQALVTALTGK
                     DKDLAAAAAGALGQMGLNDQTKSALLAAARDNPGVKMQVMQQFLQNGAPEGIRMAEEM
                     LSKKDETGMASSIIYQVANLGTAESKALLLKAVESKDTSVKYAAISALGQNPDDQNTD
                     ALLRLTRDSDATVRSMALQTLGQVGGERAQNAIMNATQSGKTEERIAAIQGLASMDDQ
                     RASQQLARLMRDSDPEVVRAAINSSYNGGAEVDQSLTAILNDANAKEDLKSAAAYQLR
                     SRGTDLDERTEQLVTKLAGAAGGYGGYGYGGYNGYYGRGEYLE"
     gene            8800..9174
                     /locus_tag="VMZ53_21730"
     CDS             8800..9174
                     /locus_tag="VMZ53_21730"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HUS31152.1"
                     /translation="MLTQEQTNELRARLQADLNRIAEHAHNGLEFTMDRDRDRVGRDS
                     MDEATEEETYSTQLRLHDRETFLLGKIQEAMRRLEAGEIDQCEECEEPIGFKRLLARP
                     VTTLCFECKTAREQVEADERPE"
     gene            9239..10579
                     /locus_tag="VMZ53_21735"
     CDS             9239..10579
                     /locus_tag="VMZ53_21735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31153.1"
                     /translation="MRDRYTITDRLDHGGMAEVFRGVAESMEGFKKNVAIKRILPNLT
                     KNQKFVAMFLDEARLSLNLQHANIVQVFDISKTADNSYYLVMEFVDGCNLKSLIERQK
                     QKTKRIEVAHSIYMMMECCKALNYAHFLENPETNEPLGIVHRDISPPNILLSKNGEIK
                     LVDFGLAKANSQIESTDPGVVKGKFSYLSPEAASGVEVDHRADVFAVGIILWELFTGR
                     RLFMGETDYQTVELVRQARVPSIAALNPEVEPELENVVRKALAKEPDDRYQSAADLGD
                     ALAQYLFSRRMKVTARDIAALVRDTQVEMMRKRSAEPKTSGLIDQLILDEMQKMTSLV
                     EGEEGKSASDSGAEGSMSLDPSNFVDTSGWAGELHNAPKRSSRASASPANKPQKAMTP
                     TPAPGEMESLEAMLEPERTGVHGKADRKTPWMLIIIIMVVLAVAAAGGVVFLMS"
     gene            complement(10576..14529)
                     /locus_tag="VMZ53_21740"
     CDS             complement(10576..14529)
                     /locus_tag="VMZ53_21740"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31154.1"
                     /translation="MSQSDFVSRGQALVAAGQFQEAVKVCRLGLLGRPTTVEGRVVLG
                     QALLALKRFDEVLAEMRVALELDHTSVPAQVLRAEALLRKGDTAPAIEALHKARHLAP
                     GDPRILQLLGEAEQGAARQSVSHPAVSFVGSGDTKHYPGHSSGGGGGNEDGSTEHFTK
                     QTSLSSPGGARRSSQRQAAVDPFEAQPAKRRVSTPSPDVLGVGDKSGTVEVDPELEGV
                     ELDNDADFDEVAAPPKSAQPASIGGGRGSVRSSSRAADGERGTIAGKKSAEQKVSTPT
                     MALDLDEEDDVEFLETRAPEERPVKRPSKGTAVRNAVAMPSGVIGEPPGPSLSDRAKS
                     KSVPPPRSKAPSAAGPPLAQTLAAMPGAVGLNPPAPPLPPAPRGPLAAALPTMAAAQP
                     PPPFVQSSPFSAQGPSPFAAHSPASNMPTMAIPASPLNPAQQQSAAAVDQLFGNEPPP
                     PPSPVWARPTMAVSVPDPRSMAAANEPTARPGELDPAILAMMSGSPSPQMHPGAMALD
                     QVSLGTPPKGQMRTGMRRTRSRLQIVLWIVVGIAVIGGGVFAGFQIRAMRLKKQIAIA
                     RSHATDLAKADTFGGWTAARDSLSGIVQASATADNRAALARARALVAYVFGDGVPEAT
                     QAAVDAAPTGNVDSGLASAYLALATNDVAAAKLAAQTTPKLSSTDPAVLYATGELAMF
                     LGDPKAAVTSLKAAADKEARPLYGIALAKAQAAVSNWDEAIAALDRVLAGNPDHPEAV
                     IARGNILAAAGRIAPGNPLGNEMRAQLERILYEGKRPVGEQTHGVSPTELAMGFLALS
                     HVDYARGDINAARRDLRAAADVNLDDQRFAEESIETLIMFGDYTLARSQLDALMKQWP
                     TSRRGRIALAQIYVAQGHAADAIELLAKAPDVLALPMGTVVHGYAQLAGGDAVNAAAD
                     FDAVLKRVPTYEPAVIGRAWIDLAAGDVDAAAKRTAERFSQRGSSVALTTAYAAALRR
                     SADPAQRDRARELLEKVVAGPPGPDTALAHLELARIYRDIGSFLEARTSYTEAGKTGS
                     VEARLEHALLVIEDHEPNRGHTMIEALLKEAGNQPSGQLVIEAVRIRQLMGDHAGASQ
                     LLELADKMSSIERWKLDRERGRLSMRRSKFTEAAAELSRALDTCGSDVETFLLASEVG
                     LIEAPLADKVKKLVAQRLKGRPEEQIILGKQLLSAGKDADAEVAYKKAQEALRNEKAS
                     RRRQAQADFGLAVIAVNRGNNVEALNKLELVIGEDPTLVDAYVFAASIGSNKKKALER
                     AQTAAELNPEYPYAWQLTGELAAKVGDKKALAEATSKLKALAPDSPEYKAVAKLR"
     gene            complement(14598..15044)
                     /locus_tag="VMZ53_21745"
     CDS             complement(14598..15044)
                     /locus_tag="VMZ53_21745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31155.1"
                     /translation="MRAILLCLVFCAAACHPPKSGDTLGDSVRRYNEGVRWGRYEVAA
                     IHVPAKERSQFVDEADQRSKDLKITDYEVVKVEQKGDRVAAVQVKMSWYKDSEGTLRE
                     TQAMQTWERHGKTWLIVDEARLRGTEMPGLREPPTTAQSSAPADQE"
     gene            complement(15041..16648)
                     /locus_tag="VMZ53_21750"
     CDS             complement(15041..16648)
                     /locus_tag="VMZ53_21750"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HUS31156.1"
                     /translation="MSKKAAATGAGAGVLYIAFAKFYFMMAGLVVQVNLLPRILSHAA
                     WGSFSFVNSLVSPVNNVLVTGSIQAVSKFAAAEPEKARRVQQAGFRMHVRLGLVVAVL
                     FIAGAPVVAWIFKDSSKTTPLMIGGLILGGNAFYAVFVGTANGLRQFQKQAGLDITFA
                     TLRSIGLVGMAMAGLGVVGVVGGWAAAVGAIVMISAAWIGLPGRDGDIGERLPVRPMI
                     KFFGGVAVYLILFNALMFVDTWLIKRLTTDYYASHMKELKDGVMQVLPWAPDVTGYHP
                     DASVLADVRVGYYAAVQNLARLSYQAIIAATFVVFPLVSRSTFTDDKDTTKRYIEVTI
                     RYSLIFAMAIAVVMAANPADILKLIYAGAPEYAQQGQWTLAFLAIGNVAFSIFAIAGT
                     ILNSAGLSRIAIATAGITLAIAAIGNAIVIPMVVDSGHTLEAAAAVTGSAMVIGAVMT
                     GVMLRRKLGAFIPAASLVRIIAATAIAMGVGYVLPLHGKLMTLVEAIIVGVTFLVVLV
                     GSRELGKRDLDAIKAVRKKRAPGGGDA"
     gene            complement(16690..17142)
                     /locus_tag="VMZ53_21755"
     CDS             complement(16690..17142)
                     /locus_tag="VMZ53_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31157.1"
                     /translation="MIRSIAALALALALAPLAGCASDTAQAKPAPTTVQASTADDPKA
                     VCVATMTRARECTDDYIPALVDARAQADAPPGIADAVKKDRNAVIAEAKQEWATDSQD
                     ANIAAMCDKMAGASTPDELATAKDCNAKTDCKEYTSCSMPLFAKHFRK"
     gene            complement(17139..17495)
                     /locus_tag="VMZ53_21760"
     CDS             complement(17139..17495)
                     /locus_tag="VMZ53_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31158.1"
                     /translation="MLDFFRAGGFNMFPLTVFGIAMLVTGIKFARNADAQRLSLIRAL
                     TTTITFCMIIGVAAGLASTAKYVVNVPEAQKDPLPYLLMGFAETMTNVMLGGSFVVLT
                     WILVAFGVRRMPKENP"
     gene            17592..18158
                     /locus_tag="VMZ53_21765"
     CDS             17592..18158
                     /locus_tag="VMZ53_21765"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS31159.1"
                     /translation="MDPDERAWCDGLRRGETAAFDAVFAAYRRRLYAYFMRMTRRRDL
                     AEDLLQETFMRLAQHAKQLAADTRLAAWLFTVAHSLVVSWGRAQAVRKALAGDLPERE
                     PADTARSPLEALADSQTQLALERAFAELPPAYREVALLVGIEGMQPSEVAAILGVRPD
                     AVRQRLARARTQLAESLGDHAPASWRTR"
     gene            18155..18376
                     /locus_tag="VMZ53_21770"
     CDS             18155..18376
                     /locus_tag="VMZ53_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31160.1"
                     /translation="MSNDDDANKLAEISRELPPLDIDDAAAQRIAQRARASVGRGPSP
                     TRFIEPVLVGLLVLSVLVWTILKLIEVLG"
     gene            18392..19036
                     /locus_tag="VMZ53_21775"
     CDS             18392..19036
                     /locus_tag="VMZ53_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31161.1"
                     /translation="MASLWQQNLRITWWLGCGAALIFLVVLPVYACSGCDIRKRSEKR
                     ACREGNVDACLAVGRYYEGKSDGLFAMVLSNGATSTEAYARACKLGSAIGCERAAFMS
                     MHADNARDPAYTHRDAAHDFASACVDGIDEACAELDDFYGDLINGTLAEDVASRYFLD
                     RCRKDNVGAACYRFGIFMKTPHSKGEVKPNPELEQQMYKEACAKGYKPGCSASR"
     gene            complement(19006..19923)
                     /locus_tag="VMZ53_21780"
     CDS             complement(19006..19923)
                     /locus_tag="VMZ53_21780"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HUS31162.1"
                     /translation="MTSVEAGMGKARDLGIALRPGLEPEQQRVEAELIRIVQDTANAD
                     AQFDALPFTQGGRILSMDYARHLSPSYQSVSERLCFTPSTRNAAAAYAKDRLERTLAS
                     SSVPPSERRLILMSGGPGSGKTTVIRGLLERALPDARIVFDCTSSNLPTSKRTLARAQ
                     EAGWSVHLVYVYCPYERAVRRVLERAARDGRYVGLGPGRSMSKISLASQQVFHELRTT
                     LGDVAGLRFLAVDMGATNGDPLPFTAATAVSPDGPCWHPPVETLLQIEREAIARFVRD
                     GGSETIVELSLRGMTGRTAAQRDALQPGL"
     gene            complement(19977..22358)
                     /locus_tag="VMZ53_21785"
     CDS             complement(19977..22358)
                     /locus_tag="VMZ53_21785"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HUS31163.1"
                     /translation="MDGTGRGQPTYGEAVTDFVAFLVKDYGHARFRDALAGYDAAAPT
                     AVFERIYGRTVAVLETEWAAAEQRGRAAIGVLGVLRAALRLWRPHWRTALSIGIALLV
                     GQAYNTFFGLSLKLVIDKAIVARDSVLLFAILGGLLVGYLFSVVAAVYADYATAKVGA
                     AVSRALRRDLFGHLQRLPVAFFVGAQSGDIMSTFSSDVTDVERAALSRLVDGVFAIVG
                     LLLNIPLLFVLDWRLSTVMLLGIPLVVLGTRITAPRSAQARYAERKALAGVVDGVNEA
                     LRAQSVVRVYGLEDHAAGRFDARLGTWSSRFVRASFLNKLVGASASLGVLLVQILVIG
                     FGGYLAFHGTLTTGSLVAFVTLNAVVCGQAYDLTRKVLPPMLSASGAMQRIDELLGEP
                     IDVADAPHAATLAPFAREIRLEDVTFSHTPDRPTLDRVSLTVPMDRTVAFVGASGSGK
                     STVLNMILRFYDAHLGKVTVDGVDVREVTQASLRAQMGVVFQDTYLYDMSIADNIRLG
                     KLGSTDDEVIAAAKAAEIHDHIASLPRGYDTFVGEGGGKLSGGQRQRIAIARALLRKP
                     AILLFDEATSALDPVAEAAINATVDRLRAGRAVALVTHRLSSARNADCIYVFDRGRLE
                     EQGTHRELLALAGRYAELWRKQSGVEIARDGQPARMTADGLRAIPLFANLADPMLETI
                     AGSLITEHYPEKDSVFEEGDLGDKFYVLARGKVEVVDVDAVLPDRRVAVLSDGDFFGE
                     MALLGDTPRSASIRTLTPCVLLSLKRERFGELMNASAELRVTVEETASRRRPQ"
     gene            22756..24078
                     /locus_tag="VMZ53_21790"
     CDS             22756..24078
                     /locus_tag="VMZ53_21790"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS31164.1"
                     /translation="MRAVIVIVICLRAWVAAADPAVLGQTPREQGMKLYSQATKMFED
                     QDFIAAAVTFGQAAQLLSRVDRDDKGGVIDKDAHTFRSAALSNKATAYSRAGLYVEAS
                     AAFAEVRDQFAAELAEKEKTEIADAIARIEERIGTVALKGLPDEELEVRFDGRFERRP
                     LDKPLRMSEGAHSLELKATGYKPYVAEVEVVGNQEVVLELKPVPLATPAKLRVESTID
                     RTSVVIDGKPSGIAPVEVSVKPGTHRVRVSAESYVAQESDVAVKPGERAIMRSGLIRA
                     RAPLGLRIQPEFLPMFAGRADTPFGKYQPAIGLSLFHDALRYRTLRFGIEVVYSPRAL
                     NSVATGMIQTWCPDRTAWKPIAWCPVNATASYVFGGEEGDFASGQARGRGFTAFEYRR
                     GAGFVRAGVGLVLEDYRKYVVEQTGERLYERFLFLWSASYELSVGLDL"
     gene            24075..25352
                     /locus_tag="VMZ53_21795"
     CDS             24075..25352
                     /locus_tag="VMZ53_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31165.1"
                     /translation="MKRLAVLALLFALENAHAEDIDDPRVLFAEGRFAAAAQVFERRW
                     QAKGDATDGVNAVVAWRTAGRYARALTLLSRVRSGKQAPTGAAATTAADLDERLGTLT
                     AMARIEGKLDPNAVIRVDGEPAERFKDMLVLDVGEHDIEIEQPDCDRFLQQVTAYPSG
                     KLVVAFKPRCDVMGTLHVVLATDPGASFTVDGVEHTTTANEADVRLPAGEHQLSLAAL
                     ERPVYDETVVVREKETTSLRIRYPWRARKLSFILGVTNEVRAGQFMVGTALGLTLGVW
                     TPSFHATFDIGAMVSNAKGLEPYYGAWGHPWLADTLVIHTPRAPVWHGKLGPYRLAFD
                     PQPLGIRFDEIRAFSYAGIRTCEEAEPRVRAWSVLPLALSADGPYAHFELTLWPASVL
                     WYHPAAALCGDDDLPSDIGFGAFVTLTGGWRLF"
     gene            complement(25349..25960)
                     /locus_tag="VMZ53_21800"
     CDS             complement(25349..25960)
                     /locus_tag="VMZ53_21800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31166.1"
                     /translation="MKRVVIAFALAGCSDPGYICNQNRAHTTWDGTCDFAPAAIQIDG
                     DLGDWAPLLLYPPDCTDCRPGEVTGVYVTETTNGEIAIFAPTVGAPVMDTRHRYDFQL
                     TPLVLPPYALEFLVTPGGVETVVGYTVSVTGVPVRAAVGPTGIELAVPISAVPFTAGA
                     HAWSVEQSFQLGTWEIAQDATPYLATVCWDPSASLCRPTQNIF"
     gene            complement(25957..26580)
                     /locus_tag="VMZ53_21805"
     CDS             complement(25957..26580)
                     /locus_tag="VMZ53_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31167.1"
                     /translation="MTRLASLLVVLAACDFGIGSDPDPNPPVKECAPGHSRATNSAPC
                     MTTMAPSIRVDGQTADWTGIPELDITGARLAFASNLDEESDVLMRAVFAGGPLDTISI
                     ELAPSSLRPASGGSDRLTIDAAGIRYEKNDMVLTPDQPPLQLASTSDGFEARIIGSWL
                     TYQGALRMRVVGSRGGGEVLRGEMIDVCFGYRSGLDALPSIACEVTR"
     gene            complement(26577..28085)
                     /locus_tag="VMZ53_21810"
     CDS             complement(26577..28085)
                     /locus_tag="VMZ53_21810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31168.1"
                     /translation="MSEASTLEGTRLGAYRIERQLGEGAMGEVFLAVHEALGRQVAIK
                     KLKPQVAADRAMTERFFAEARAVNIIRHENIVECTDLVNEGGTSYIVMELLEGKTLSV
                     AIRQAKQMPAVRAAKIAAQIADALAAAHTKGIVHRDLKPDNVFLIKRAGSADYVKVLD
                     FGMARLRPELGGVGATQSGALIGTPAYMAPEQARGEKVGPAADIYALGVVLFHMLTGQ
                     LPFAAESLTMMLIQIVQEMPPRIDKLAKDVPPALVALVARCLDKDASKRPPDMATLRR
                     ELFEAVGLPVETAQSVAASQSPLASGNASIALTETMESGPRPDVNPLAETSAPGLGAT
                     TAPMWSGQSSLTAAAAEVSTAPKRRPRWPMIAAAGGALAIVVGVGVVVTNKHTPESKS
                     SAPAARPADPPQTAAPAPSAPTPPVAAPVVEPAGAGSAVGSASAIAAVPTETTVPKKT
                     PKKTTAKIATTPKAGSGAGSAVPDITKPPVETKSPEEKIDRTKQLVGPKPGS"
     gene            complement(28116..>30900)
                     /locus_tag="VMZ53_21815"
     CDS             complement(28116..>30900)
                     /locus_tag="VMZ53_21815"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUS31169.1"
                     /translation="DRMPPKIVAPVTAVGAAVLLVAARFALGPHWLYYVLYAGLDICA
                     VLLSMQFWLAAGERFSAREAKKVFTIIAASGTAATITVGLVVSAIVSRTGAENLLFGC
                     AAFQIVVAVLATVLGRRPPTEVAARPRVQQTSGQPAGSRHLDLLGPLLMFGMLGVTLI
                     DFQFKSIANAAYAGDRVGMVRFFGLLYVVTAAAALVIQLLFTRKILARFGVAGALLLL
                     PVTLGLGAIALLLAPSLFTATASKAADTTFRSSVDSSGQQLLYLPAPPAERSRRKRRI
                     DMLIKPGTEGSVGVALLAYKSYLTTRTPLAIAAIVAITAWIALVLRMRGAYVKSVADT
                     LRRRRIPAPDEEGDTGLAAEASRAIKQALSSTDAAVVRRGIDLARLDPAGVRDELRDL
                     LTHSDAAVRGDAFACLADVAGGLTDTELAAALDGADPTMAASAAATMYRRGDLDRRTE
                     MVERARAWLADPVKQALGFAIAEQIADVRLAEPVRVHLGSRHARHAIRALVAIGEPAL
                     AHVHPLLDVDDDAVAAEAIRIVGMIAAPDAVDRLVSILQDKREVRRAAAAAALARLRR
                     DHPDRSPPPAAIVDACMTEISRAFVAMAAAAGLGHAESIMKPDGTRIEVPFVTDDLAG
                     PAALLSRALRERSERARDRVFDLLAALEPELEIATVRANLRDPDPTRRANAVELLDAR
                     SWPRSVAELKPLALAIVDDSSRDAKLAAASKKLRLPRYDRAGWIDNLLADRNPWIRAC
                     TAYYAGAARDQGARPRIEKLTTDPNPLVAETARHALASIDRTPETKKMLTTAEKVLFL
                     KGAELFAAIPAEDVAAIATAAEEVSFEEGEVIFAAGDPGDSLYLVVEGKVRIAAGERT
                     LAELGTREVFGEMAILDPAPRSATAVATAPVVAVRLGQDDFSDVLAERPEVAAGVLRV
                     LVRRLRTANVASG"
BASE COUNT         5100 a        10041 c        10441 g         5318 t
ORIGIN      
        1 cgggcgctgc ggtcgttcgc ggtgatcgcg cgaagacgga tccgccggcc gaggcgtcgg
       61 agccgacaag cggcccgagc ccgacgacga actaatcttc tccgactccg agtccgactc
      121 cgactccgag tccgactccg agtccgggtc cgagtccgag tccgagtccg agtccgagtc
      181 cggatccgag tccggatccg agtccggatc cggatccagt gcggagcgcg gcgccggggc
      241 gcgcgcgcct cagcggcggc tgcgttgcag gccgtagatc atgccgcaga ggcgatcgcg
      301 ctctgccgcg agcggcggcg agtcgagcca gcgatgcgcg gcagcgatgt cgagcaccgc
      361 gccgacttcg cggaggctgc cgagcgcgat gcggaagtgg tgaaggcggt cgccgccttc
      421 gcggccgttg ccttcggcga ggttgagcag gaccgagtcg atcgcgttcg cgccttgctt
      481 cgcgagcgag gcgttgtggc ggcggatgat cgcgagcgat gcgtgcagcg aggtgatgat
      541 ttcgagagcg aggtcgtagg cgagaaagcg catggtgatg actccggtgg ggttcgcgcc
      601 cgcgcgcgca ccgcggaaag aagcgggacg cgcgctgaaa ggcggacgcc gcggctgccg
      661 tcgagcggtc gcgcgcgcgg accgcttctt tacgcgaagc gcgcagcgac cccaccggag
      721 gcgaggcatg cgcgactcgc cacgagctcg cgcaagatca gcgcgccgcg agctcgcgag
      781 cccgacgcac gcgcgagcga gcggagtcgc gacgggcgga ctacggcgcg ggcgcgggcg
      841 cggcggccga gccggcgtcg ccgggcggcg gcgcttcgag gtcgaggccg aagcctttgc
      901 actgcgcgac gacgtcgggg cgcgcggact tcgcgatcgc gttgacccag cgcttcgcca
      961 gcgcggtgtt gttcgaacga catgccgaga gcgcgcaggc ttggagaatg gctggcgaga
     1021 agatcaggtt cttggtgcac gccttgttcg tcgtcgcgaa gtcgcccttc gcgtagagct
     1081 cttcgatcga caattccttc gcgggctcgg tcttgttgcc ggtgttggtc ttgtcgcctg
     1141 tgttcgtctt gttgccggtg ttcgtcttgt cgccggtgtt ggtcttgtcg cccgtgttcg
     1201 tcttgttgcc ggtgttggtc ttgtcgcccg tgttcgtctt atcgttctga gcaacgacgg
     1261 cggcatcgac cggcggcgcg acgacgaccg cgaccgcagc atcgacgggc ggcgtttccg
     1321 cgacgacggc ggcatcgatc ggcgtctccg cgactgccgc atcgactgat ggcggcgttt
     1381 gcatcgtcgt tgtcgtcgtt tgcgagcccg cggcggcgct gcccgcctct tgcgtcttcg
     1441 tgttcgcggt attcgtattc gtattcgtgt tcgtgttcgt cttgtcgcct tggttctgtg
     1501 cgacttgatc gggcttggtc ggcttgtcgt tgccgtcgcg acctttgagc acgaagatca
     1561 ggatgagggc gaccgcagcg acgaggagtg ctgggacgag ccatcccgga cggcgggacg
     1621 gcgagtcgtc ggcatcgacc gcagtgcgtg ccgcagcgcg ccgcgaggcg gcagcagcgt
     1681 cagtgtcatc gtcgtcgtcg tctttgacgc tggccttcgc tgatacgcca ttggacttcg
     1741 atgcggtgcg cgagacggcc ggcttgtcgt cgtcggactt tttcgctgac ttgtcagcgg
     1801 gcttgtcggc cttcgtctcc gctttcgcct cggcctttgc atccgccttt gcttcggcgg
     1861 ccgcatcgga tttgggctcc gcggcgattt ctaccttcgc gtcgctcgtc gatggaccat
     1921 cagatgacgc cggcttcgag ccaatctcgc ccgatgtggc cgccgtctgg gatgcgactg
     1981 caacttcgcc tgcggcggcg acggccgcgg cgtcgtacgt cgcggtgacc gcgcgattgc
     2041 cgacgacgac tgcaccgtta ctgccgttcg cgccgttgga gtccagggcg gtggcggtgg
     2101 cggtggacgc ggcttcggcg tgtgcgctcg cggcttcgag ggctttgagc gcggcactcg
     2161 cgtctgcggc ggcggcggtg gctttgaccg tcggcgcgga ggccgtttcg gcgacacgcg
     2221 tgatgtacga gtactggttc gtcggcgtca gcgtttcggg ccgcttgcga acggagcccg
     2281 tgtcggcggg ggacgcgacg gcagcgggaa cgcccgacgg tggtgtggcg ttcgcggatg
     2341 cggtggcgat gcccgacggc ggggtggcac cgaccgcggc aatttggtcg gagacgggcg
     2401 ggcgcatcgt ctctttgcgc ttgcgcgctt gctcgaggat gcggtcgcgg atctgcgcga
     2461 agtcttcgcg tgcgttttcg acttcgccgg cggtcggcgg tgaggtggct gggcgatcgc
     2521 cacggctcga accgccgatg cgttgcgcga gcgagcggat ctttgcggcg gcctcgcgaa
     2581 cgctcgcgag cagctcgtcg atcgggtccg cggtcgtggc ggatgtttcg gagaggatgt
     2641 cttcgctcgt gacttggatc ggcttgagat cgtcggcttc ggtggccgac gtcggcgggc
     2701 caaaccagag gcgcatcatg cgcgcgaggt cgttcgtcga gagcgagaag ccgagcttcg
     2761 tcgagagcga ttcgatcgcc gcgagcagct cgccgccggt ttggaatcgg tcgtcgggct
     2821 ttttcgcgag cagcttgacg aggatttggt cgagctcggg cggaacgtcg aagcgacgca
     2881 gcgacggtgc tggcgtgtcg tcattctcga tcgcttggcg tgtttcctcg tcggtggggc
     2941 gatcgtggag gtcaccgagt gtgaggagct cccagagcac gacgccgagc gagaagagat
     3001 cgctgcggcg gtcgagcttg tcgccgcgaa cttgttctgg cgatgcgtag acgagcttgg
     3061 ccgggccgcg gccgcgcgcc atgccgaagt cgaggacttt gacgatgcct tcacggctga
     3121 tcatcacgtt cgttgccgag atgtcgccgt ggatgatgtt gagcgacttg ccgtccatgc
     3181 cgttgcgctc gtgcgcgtgg tggaggccgg cggctgcgcc cgctgcgatc gtgaggaagg
     3241 cgcggattgg aacctggatt tttttgccac gtgcgtgcgc gatgagcgcg cggatcgtct
     3301 cgccgtcgac gtactcggtc gtgaagaagt acgagccgcc gagctttccg acttcgtaga
     3361 cctgcgcgac gtttgggtgc gcgagcttgg cggcgacgcg tgcttccgcg aggaaggctt
     3421 gggcgacggc cttctctttg gccgcatcgc gccgcagtcg tttgatgacg accagccggt
     3481 cgactccggt ggcacgcgcg gcaaacagct ccgcggtccc cgagccttgc gagagcttcg
     3541 ctaggatctc gtagctactg gctgtggaac ctgcctgcgc gtcggacacg gtctagcatt
     3601 gtagccgagg ccggggcgga gatccggcga ggagccgagg atcctggggt agccacgggt
     3661 gccactcgtg tttttcggcg tctcggggcg acgtagcgtg tgagcggcta cacttgcccc
     3721 ggctcgatga acgtcgcgac gcgaatcacc ctggcgacgg cggtcgtggt cgccctcgca
     3781 agtgcggcgt acgcggcgtt cgacctccgt gctcggcgcg ccgaacgcct cgaacagctc
     3841 ggtcgcgaag cgaaggcggt cgcgggcgcg gtgcggttcc agctcgaagg tcagggctcg
     3901 gcgttcacgg cgccgagcga caagtggctg cgagatctcg agatcaagag cggttggaac
     3961 atcaaggtcg cgccgcgggt ccgcaaggat ctgccggcgg ggccggactt gagcgaacgc
     4021 gagctcggct ggctgcgcgc gctcgtggag agcccgacgt ttcagctcac cgacaacgag
     4081 atcggcgatc gctactacta cgtgcttccg ctgcgcgccc ctgccctgcc gagtgaggag
     4141 ccgacgatcc tcggcatgct ggagctgaac aagtccgtcg cggtcacgag cagtgacgac
     4201 ttgggccggg cggtcgcgct cgtcgtgctc atcgttcttg tcacgacgct catggtcggc
     4261 gcactggcga gccgctttgt cagccggccg atcacgaagc tgctgcgcgg catcgacgac
     4321 gtcgccaaag gcgatctctc gcacgtgatc ctcgccgagc gcgacgacga gatcggacag
     4381 atcgcgacgc gcttcaacga gatgacgttt tcgctccgcg agtcacgcgg tgaaacgcag
     4441 cgtcagaacg aagcgaagct cgcgctcgaa cagcggctcg gccagaccga gaagctcgcc
     4501 acgatcggtc agctcgccgc cgaaatcgcg cacgaggtcg gcacgccgct caacgtgatc
     4561 gcgggtcgcg cgaagtcgat gaagcgcaag tcaaaggacc ccgaggccgt cgagaagaat
     4621 gccgagatcg tggcggagca gaccgctcgc atcacgcgaa tcatccaacg cctgctcgac
     4681 ttcgcccgcc gcaaggtcgg cacgaccggc aaggcggaga tcaacctcaa cgagctctcg
     4741 ctcacgacga tggagatgct cgcgggtcag ttcgccgcgg cgaaggtaaa gacgaagctc
     4801 gaccgcgcgg agggtctgcc aaaggtcgcc ggcgacgcgg accgcttgca gcaggtcctc
     4861 atcaacctct tgctcaacgc ggtgcaagcg atgcccgacg gcggctcgct catcgtcgag
     4921 acgcgagcgg tcaagcgcac gcgccccggc ctcgagagtg ggcccgagca gagcttcgtc
     4981 ggcatcgccg tgcgcgacac cggcatcggc attcccgagg acatccgcga gaaaatcttt
     5041 gatccgttct acacgacgaa ggaaggccaa ggcggcaccg gacttggcct cgcggtctgc
     5101 tccggaatcg tcaaagagca cgacggatgg atcgacgtcg aagccaacga caacggcggc
     5161 acggtgtttc gcgtctactt gcccgcgtag cgattgggct tcgcgctcgt gattttccgt
     5221 cgacggatct ggcatgctcg ccgcgcgtgc agcgggttca tctcgagtat ctgggtgaca
     5281 gcgtggagct gccggttggc gagaccgtca tagggcgtga tctctcgtgc gcgctgcggt
     5341 tcaacgatgc ggcggtctcg cgtcgccacc tgcgcttcat tcgccgccag aacgaagtct
     5401 ttctcgagga cctcggatcg agcaacggca cgctgctcaa cggccgcaac atcacgggcg
     5461 cgctgcgcat cacggatggc gacgagattc gggtcggcag ccgcctgctc acgattcgca
     5521 tcgtcgaggg cgaggaagag cccgcggcgt cgacgctgat cctcaagaac ttcaacgcgc
     5581 ccgaagagct gaggaagctc cgcgccacga cctcgcaggt cgccgtcacg caaccgccgc
     5641 cgatgaacgc gaaccagcgc tgcccgcgtt gcgctgccgc cgtgaacatc gaggacgacg
     5701 agtgcacgaa ctgcaagttc cgctgggcgg actttcgccc gcagagccgc accgacgttc
     5761 gcccgaaccc gctgaatcgc cgccgccacg atcgcaaacc gatcgagctg tcgctcgtct
     5821 actcgagcag cgagctcgag atcgaagcga cgtcgcgcga cctcagcgag agcggcgtgt
     5881 tcgtctgctc gcaggtgctc gatccgatcg gcaccgagtg ccagctgacg attctcgtcg
     5941 acggtggccc accgatcggc gtccgcggcg tggtccgtcg cgtcgtcgag cgggagacgc
     6001 tcgatggcga gccgatcggc atcggcgtcg agttcgtgaa gacaggcgaa gccgagctgt
     6061 cgtggattcg cacggtcgtc agccggatga tgcaggaagt cgaagacgac gagacgcagc
     6121 ccgtcgacgt ccgctgacta ctcgagatat tcgccgcggc cgtagtagcc gttgtagcca
     6181 ccgtagccgt agccgccgta gccgccggcc gcgcccgcga gcttcgtcac gagctgctcg
     6241 gtgcgctcgt cgagatccgt gccgcgcgag cggagctgat acgcagccgc gctcttgagg
     6301 tcttccttcg cgttcgcgtc gttgaggatt gcggtgagcg actggtcgac ttcggcgccg
     6361 ccgttgtacg aggagttgat cgcggcacgc accacttcgg gatcggagtc gcgcatgagg
     6421 cgagcaagct gctggctcgc gcgctggtcg tccatcgagg cgaggccttg aatcgcggcg
     6481 atgcgctcct cggtcttgcc cgactgcgtc gcgttcatga tcgcgttctg cgcgcgctcg
     6541 ccacccacct gcccgagcgt ttgcagcgcc atcgagcgca ccgtcgcgtc gctatcgcgg
     6601 gtgaggcgca gcagcgcgtc ggtgttctga tcatcggggt tctggccgag cgcgctgatc
     6661 gcggcgtact tcacgctcgt gtccttcgac tcgacggcct tgagcagcag cgccttggac
     6721 tccgccgtgc cgaggttcgc gacctggtag atgatggagc tcgccatccc ggtctcgtcc
     6781 ttcttcgaca gcatctcctc cgccatccgg atgccttccg gcgcgccgtt ctggaggaac
     6841 tgctgcatca cctgcatctt cacgcccggg ttgtcgcgag ccgcagcgag cagcgccgac
     6901 ttcgtctgat cgttgaggcc catctggccg agcgcaccag cggccgcagc cgcgaggtcc
     6961 ttgtccttgc cggtgagcgc cgtgacgagc gcttgccgcg cgtcttccgt gccgacgcga
     7021 ccgaggacgc tcgctgcata gttcgcttct tcgcggcgac cgctgaacag cgcgtccgac
     7081 agcatctggc cgacggccgg atccgccggg tggctcgtcg cgaggtactc gagcgcctgc
     7141 acgcgcgagc tgccgtgcga cttgctcgcg atatccatga gcgcgtcgaa cgctttcggc
     7201 gtgcctgcat caccgaggac gcgcatcgca tcgtagcgct cgttgcgcga gcccttctgc
     7261 gcgaggtcga tcgcgagctg aatcgcttct tgattgcccg cgcgcacgag gcgcggcagc
     7321 accgcacgac gctcctggct cgagccctgc ttcacgacgc tgatgagcgc ctgctcgacg
     7381 tcctcgccct gcatctgcgc gagctggtag agcgcgcccg tcatgttcgc gcgatccgag
     7441 agtgcagcct cgatgagcgc gtcgcgcgca tcctggccgc cgatcgtcgc gagcgcttgc
     7501 accgcggcgc tggccgactg acggtcgccc gacttcaaga tctcgacgag gatctgcgtc
     7561 gccttctcgc cgccgacgtc accgatcgcg ctgaccgcgg tccagcgaac gttgccgctg
     7621 ccgtgcagcg ccgcttcctt cagcgccggg aggccttctt cgccggcatg tccgagcgcg
     7681 gcgagcgccg cattgactag ctgagggtcg cccgacttca cgatcgccgt gagcttcgcg
     7741 agcatcggtg catcgaccgt atcgatcgac gcgatcgcag ccgccgcgac gcgcgtgtcc
     7801 gccgagtcca tcagcttgcg cagcgcggtc tcggccgccg gcgtgcctgc gctaccgagc
     7861 gcgtagacgg ccgtcgacga aacttggtag tcgctcatgt tcgcgagctc gatgagacgc
     7921 gcgaccgcgt tgtcggtgcc gagcgtgccg agcgcgctga tcgcggtcgt ctgcgccgga
     7981 tcgccttgcg cgcgagagag ctcgacaagc gtcttctccg cggcttcact gtgcgtcgcg
     8041 ccgagcgcgt agatcgccga gctccgaacc gtcgggcgct cgtccttcgt atgcgccacg
     8101 atcacgtcga tcgccttctg cgtaccgatt tgaccgaacg ccgagagcat cgcctcggga
     8161 acgccatggc gcgggtcttc gactagtggt gcgagcgtct cgactgcatc gtcgtcaccc
     8221 acgacgccga gcttgtcggt cagcagcgcg atgtccttga cgctcttggc cgcgaggatg
     8281 cgatcgatga gatccttctg cgagatgccg agcgcctcgg ggctcacgtg gatcggcttg
     8341 cggaacacct gcggcatcgc tccgcgcggc gcggctgctt gctttgcggg gacgttgtgg
     8401 ttcccgccct tgtgagcgtg aacaacggcg ccaccggcca gggcggcaaa cagggcacag
     8461 gcgagcttcc agcgggtcaa agtggtcaca gagggctcct cgggggctgt cgaatcgtct
     8521 gtgtcaaacg aacgccgatt ggtatcgatc cattccgtgg ctcgcctaag gaaccgcgcg
     8581 gaaagaccgc aaaattggac atcccacctg ccgccaggtt tcggcgatcc gagggaataa
     8641 atcgtccacg gtagagcgag ccttggcgga gcgcgcggtc gcatgaaatc gccgccggac
     8701 gcaagcgcac accgaggcat cagagcgcgt tcttcacgat gatctcgatg cggcgtcttc
     8761 gtcgattgac gctcccaccg acgtccgtag actgaacgca tgctgactca ggagcagacc
     8821 aacgagcttc gtgctcgcct gcaggcggac ctcaaccgga tcgccgagca cgcgcacaac
     8881 ggcctcgagt tcacgatgga ccgcgaccgc gatcgcgtag gtcgtgactc catggacgag
     8941 gccaccgagg aggagacgta ctcgacgcag ctgcggttgc acgaccggga gacgttcctg
     9001 ctcggcaaga ttcaggaagc catgcggcgc ctggaagccg gtgaaatcga ccagtgcgag
     9061 gagtgcgagg agccgatcgg gttcaagcgc ctgctggcac ggccggttac aacgctttgt
     9121 ttcgaatgca agacggcccg cgagcaagtc gaggccgacg agcgccccga gtagcacggt
     9181 cctaccccgg cactttgcta gggttaccgc gcatctggta gtatttcgtc cgtcgaggat
     9241 gcgcgaccgc tacacgatca ccgaccgact cgaccacggc ggtatggccg aggtcttccg
     9301 cggggtcgcg gagtcgatgg aaggcttcaa gaagaatgtc gctatcaagc gcatccttcc
     9361 gaacctgacc aagaaccaga agttcgtcgc gatgttcctc gacgaggcgc gactgagctt
     9421 gaacctccag cacgcgaaca tcgtgcaggt cttcgacatc tcgaagacgg ccgacaactc
     9481 ctactacctg gtgatggagt tcgtcgatgg gtgcaatctc aagtcactca tcgagcgcca
     9541 gaagcagaag accaagcgca tcgaggtcgc ccactccatc tacatgatga tggagtgctg
     9601 caaggcgctg aactacgcgc actttctcga gaaccccgag acgaacgagc cgctcggtat
     9661 cgttcaccgc gacatctcgc cgccgaacat tcttctgtcg aagaacggcg agatcaagct
     9721 cgtggacttc ggcctcgcga aggcgaacag ccagatcgag tcgaccgatc cgggcgtcgt
     9781 gaagggcaag ttcagctacc tgtcgcccga agcggcaagc ggcgtcgagg tcgaccaccg
     9841 tgcggacgtg ttcgcggtcg gcatcatcct gtgggagctg ttcaccggcc gccgcctgtt
     9901 catgggcgag accgactacc agaccgtcga gctcgtgcgc caagcgcgcg tgccctcgat
     9961 cgctgcgctc aatccagagg tcgagcccga gctcgagaat gtcgtccgca aggcgctcgc
    10021 gaaggagccc gacgaccgct atcagagcgc cgccgacctc ggtgacgccc tcgcgcagta
    10081 tctgttcagc cgccgcatga aagtgacggc gcgcgacatc gccgccctcg tgcgcgacac
    10141 gcaggtcgag atgatgcgca agcggtcggc cgagcccaag acgtcgggct tgatcgatca
    10201 actgatcctc gacgagatgc aaaagatgac gtcgctcgtc gagggcgaag aaggcaagag
    10261 cgcgtccgac agcggcgccg agggctcgat gagcctcgac ccgtcgaact tcgtcgacac
    10321 gtcggggtgg gccggcgagc tgcacaacgc gccgaagcgt tcgagccgcg cgagtgcatc
    10381 gcccgcgaac aagccgcaga aggcgatgac gccgacgccc gctccgggcg agatggaatc
    10441 actcgaggcg atgctcgagc cggagcgcac gggcgttcac ggcaaagcgg atcgcaagac
    10501 gccctggatg ctgatcatca tcatcatggt cgtgctcgcg gttgcagcgg ccggcggcgt
    10561 cgtcttcctg atgagctaac gcagcttcgc gacggccttg tactcggggc tgtctggcgc
    10621 gagcgctttg agcttgctcg tcgcttcggc gagcgctttc ttgtcgccga ccttcgctgc
    10681 gagctcgccg gtgagctgcc acgcgtaggg atactcggga ttgagctcgg cggccgtctg
    10741 tgcgcgctcg agtgccttct tcttgttcga cccgatgctc gctgcgaaca cgtaggcgtc
    10801 gaccagcgtg gggtcctcgc cgatcacgag ctcgagcttg ttgagtgctt cgacgttgtt
    10861 gccgcgattg accgcgatca cggcgaggcc gaagtcggct tgcgcctgac ggcgacgcga
    10921 ggccttctcg ttgcgaagcg cttcttgcgc cttcttgtac gcgacctccg cgtcggcatc
    10981 cttgccggcg gagagcagct gcttgccgag gatgatctgc tcctccggcc tgcccttcag
    11041 gcgctgggca acgagcttct tcaccttgtc agcgagcggc gcttcgatga ggccgacctc
    11101 ggacgcgagc aggaacgtct cgacgtcgga gccgcaggta tcgagcgcgc gagacagctc
    11161 ggccgcagct tcggtgaact tcgagcggcg catcgacagc cggccacgtt cgcgatcgag
    11221 cttccagcgt tcgatgctcg acatcttgtc ggcaagctcg agcagctgcg acgcgccagc
    11281 gtgatctccc atcagctgac gaatgcgcac ggcttcgatc acgagctgac cgctcggctg
    11341 gttgccggct tccttgagca gcgcttcgat catcgtgtgc ccacggttgg gctcgtgatc
    11401 ctcgatgacg agcaacgcgt gctcgaggcg cgcttcgacg ctgccggtct tgccggcttc
    11461 ggtgtacgac gtgcgcgctt cgaggaacga gccgatgtcg cggtagatgc gtgcgagctc
    11521 gaggtgtgcg agcgcggtgt cgggtcccgg cggaccggcg acgactttct cgaggagctc
    11581 gcgcgcgcga tcacgttgcg cggggtctgc gctgcgacga agcgccgcgg cgtatgccgt
    11641 ggtgagcgca acgctcgagc cgcgctggct gaagcgctcg gcagtccgct tcgcggccgc
    11701 gtcgacgtca ccggcagcga gatcgatcca cgcgcgtccg atgacggcgg gctcgtacgt
    11761 cggtacgcgc ttgaggacgg cgtcgaaatc ggctgcggcg ttgacggcat cgccacccgc
    11821 gagttgcgcg tagccgtgca cgacggtgcc catcggcaac gcaaggacgt cgggtgcctt
    11881 cgccagtagc tcgattgcat cggcggcatg gccttgcgcg acgtagatct gcgcgagcgc
    11941 gatgcgtcca cgacggctcg tcggccactg cttcatgagg gcgtcgagct ggctgcgcgc
    12001 gagcgtgtag tcgccgaaca tgatcagcgt ctcgatgctc tcttcggcga agcgttgatc
    12061 gtcgaggttg acgtcggcag cagcgcgcag atcgcggcgt gccgcgttga tgtcaccgcg
    12121 cgcgtagtcg acgtgcgaga gcgcgaggaa gcccatcgcg agctcggtcg gagagacgcc
    12181 atgcgtttgc tcgccgacgg gacgcttgcc ctcgtagagg atgcgctcga gctgagcgcg
    12241 catctcgtta ccgagggggt tgcccggtgc gatccgaccg gcagcggcga ggatgttgcc
    12301 gcgtgcgatc accgcttcag gatgatccgg gttacccgcg agcacgcgat cgagcgcggc
    12361 aatcgcttcg tcccaattcg agacagcggc ctgcgccttc gcgagcgcga tgccatacag
    12421 cgggcgtgct tctttgtccg ccgcggcttt cagcgaggtg acggccgcct tcggatcgcc
    12481 gaggaacatc gcgagctcgc cggtcgcata gaggacagcc ggatccgtcg acgacagctt
    12541 cggcgtcgtc tgcgctgcga gcttcgcggc ggcgacgtca ttggtcgcga gcgccaggta
    12601 cgcacttgcg agacccgaat cgacgttgcc agtcggcgct gcatctactg cggcctgcgt
    12661 cgcttccggc acgccgtcgc cgaacacgta tgcgacgagc gcacgcgcgc gggcgagagc
    12721 tgcgcgattg tcggcggtcg ccgaggcctg gacgatgccc gacaagctgt cgcgcgctgc
    12781 ggtccagccg ccgaacgtgt cggcctttgc gaggtccgtc gcgtgcgagc gagcaatcgc
    12841 gatctgcttc ttcaggcgca tcgcgcggat ctggaatccg gcaaacacgc cgccaccgat
    12901 cactgcgatt ccgacgacga tccacagcac gatctgcagg cgtgatcgcg tacgccgcat
    12961 gcccgtgcgc atctgtccct tcggcggcgt gccgagcgag acttgatcga gcgccatcgc
    13021 gccggggtgc atctgtggcg aaggcgagcc cgacatcatc gcgaggatcg cgggatcgag
    13081 ctcaccggga cgcgcggtcg gctcgttcgc agcggccatc gagcgtggat cgggaaccga
    13141 gaccgccatg gtcgggcgcg cccacaccgg cgatggtggt ggcggcggct cgttgccgaa
    13201 cagctgatcg accgccgctg cactctgctg ctgcgcgggg ttcagcggcg atgcaggaat
    13261 cgccatcgtt ggcatgttcg aggcaggcga gtgcgcggca aacggcgaag gcccctgtgc
    13321 cgagaatggc gagctctgca cgaacggcgg cggcggctgt gcggcagcca tcgtcggcag
    13381 cgcggcggcg agcggtccgc gcggcgcggg aggcaatggc ggcgcgggtg gattgagccc
    13441 gacggcaccg ggcatggcag cgagcgtttg tgcgagcggt gggcctgctg cgctcggcgc
    13501 cttgctgcgc ggcggcggca cggacttcga cttcgcacga tccgagaggc tcggtcccgg
    13561 cggctcgccg atcacgcccg acggcatcgc gaccgcgttg cgcacggccg tccctttgct
    13621 cggccttttt accggacgct cttcgggtgc acgcgtctcg agaaattcga cgtcatcttc
    13681 ctcgtcgaga tcgagcgcca ttgtcggcgt gctgaccttc tgctccgcgg atttcttgcc
    13741 ggcgatcgtg ccgcgctcgc catctgcggc gcgcgacgac gaacgcaccg agcctcggcc
    13801 accgccgatc gatgcgggct gcgccgattt cggcggagcg gcgacctcgt cgaagtcagc
    13861 gtcgttgtcg agctcgacgc cctcgagctc tggatcgact tcgacggttc cggacttgtc
    13921 gccgacgccg agaacgtcgg ggctcggcgt gctgacgcgg cgcttcgcgg gctgcgcctc
    13981 gaacggatcg accgcggctt gccgctgtga cgaacggcgc gcaccgcccg gcgatgacag
    14041 cgatgtctgc ttcgtgaagt gctccgtcga cccatcctcg ttgccgccgc cgccgccgct
    14101 cgagtgcccc gggtaatgtt tcgtgtcgcc gctgccgacg aagctgaccg cgggatggct
    14161 taccgactga cgcgctgcgc cctgctccgc ttcgccgagc agctggagaa tgcgcgggtc
    14221 acccggcgcg aggtgccgcg ccttgtgcag cgcttcgatg gccggcgccg tatcgccctt
    14281 gcgaagcagc gcctcggcgc gcagcacttg cgcaggaacc gacgtgtggt cgagctcgag
    14341 cgcgacgcgc atctcggcga gtacttcgtc gaaccgcttc agcgcaagca gcgcttgtcc
    14401 gagcacgacg cggccttcga ctgtggtcgg tcgaccgaga aggcccaggc ggcagacttt
    14461 gacggcctcc tgaaactgcc cggcggccac cagagcctgc ccacggctga cgaagtcgct
    14521 ctgcgacact tccagtccgt gatgttacgc catctgcgac cacggcggca cctgctggcg
    14581 cacggccgat gtgggcgtta ttcctggtcg gccggggcgc tcgactgcgc ggtcgtcggg
    14641 ggttctcgca gacctggcat ctcggtaccg cgcaggcggg cttcgtcgac gatgagccag
    14701 gtcttgccgt gccgctccca ggtctgcatg gcctgggtct cgcggagcgt gccctcggag
    14761 tccttgtacc aggacatctt cacctgcacc gcggcgacgc gatcgccctt ctgctcgacc
    14821 ttgaccacct cgtagtcggt gatcttgaga tccttcgagc gctggtccgc ttcatcgacg
    14881 aactgcgatc gctccttcgc cggcacgtgg atcgcggcca cttcgtagcg gccccatcgc
    14941 acgccctcgt tgtagcggcg caccgagtcg ccgagcgtgt cgcccgactt cggcggatgg
    15001 catgccgccg cgcagaacac cagacacaac aggatggctc tcatgcgtca cctccaccgg
    15061 gcgcgcgttt cttgcgcacc gctttgattg catcgagatc gcgtttaccg agctcgcgtg
    15121 aaccgacgag cacgacgagg aacgtcacac cgacgatgat cgcttcgacg agcgtcatca
    15181 gcttgccgtg aagcggcagc acatagccga cgcccatcgc gatcgcagtc gccgcaatga
    15241 tgcgcacgag gctcgctgcg gggatgaatg caccgagctt gcgccgcagc atgacgcccg
    15301 tcatcacggc gccgatcacc atcgcgctgc cagtcacggc ggctgcggcc tcgagcgtgt
    15361 gaccgctgtc gacgaccatc gggatcacga tcgcgttgcc gatcgcagcg atcgcgagcg
    15421 tgataccggc cgtcgcgatc gcgatgcgcg agagtcccgc actgttgagg atcgtgcccg
    15481 cgatcgcaaa gatgctgaat gccacgttac cgatcgcgag gaacgcgagc gtccactgcc
    15541 cttgctgcgc gtactcgggc gcaccggcgt agatgagctt caagatgtca gcggggtttg
    15601 ccgccatcac tacggcgatc gccatcgcga agatcagcga gtagcggatc gtgacctcga
    15661 tgtagcgctt cgtcgtgtcc ttgtcgtcgg tgaacgtcga gcgcgacacg agcgggaaca
    15721 cgacgaacgt cgctgcgatg atcgcttggt acgacaggcg cgcgaggttc tggaccgctg
    15781 cgtagtaacc gacgcgaacg tcggcgagca cgctcgcatc ggggtgatag ccggtgacgt
    15841 cgggcgccca cggcagcacc tgcatgacgc cgtctttgag ctccttcatg tggctcgcgt
    15901 agtagtccgt cgtcaggcgc ttgatgagcc acgtgtcgac gaacatcagc gcgttgaaca
    15961 ggatcaggta cacggcaacg ccgccgaaga acttgatcat cggccggacg ggaagccgct
    16021 caccgatgtc gccgtcgcga ccgggcagac cgatccaggc cgcggagatc atcacgatgg
    16081 cgccgacggc agcggcccac ccaccgacca caccgacgac accaagcccc gccatcgcca
    16141 ttccgacgag gccgatcgag cgaagcgtcg cgaatgtgat gtcgaggccc gcctgcttct
    16201 ggaattgccg caggccattc gcggtgccga cgaacacggc gtagaacgcg ttgccgccga
    16261 ggatgaggcc gccgatcatg agcggcgtcg tcttgctcga gtctttgaag atccacgcga
    16321 cgacgggcgc ccccgcgatg aacagcacag cgacgacgag gccgagccgc acgtgcatgc
    16381 ggaagccggc ctgctggacg cgtcgcgcct tctccggctc ggccgccgcg aacttgctca
    16441 ccgcctggat cgatccagtg acgagcacgt tgttcaccgg cgacacgagc gaattgacga
    16501 acgagaagct gccccacgcg gcgtgcgaga ggattcgcgg cagcagattt acctgcacga
    16561 cgaggcccgc catcatgaag tagaacttcg cgaacgcgat gtagaggacg cctgcaccgg
    16621 cgcccgtcgc tgcggccttc ttagacaaca gatcccgcgt aacacgcgag atctacggcg
    16681 gcaacaaagc tacttacgga agtgcttcgc gaagagcggc atcgagcacg acgtgtactc
    16741 tttgcagtcc gtcttcgcgt tgcagtcctt cgcggtggcg agctcgtcgg gcgtcgacgc
    16801 gccggccatc ttgtcgcaca tcgccgcgat gttcgcgtct tgcgagtcgg ttgcccattc
    16861 ctgcttcgct tccgcgatca ccgcgttgcg atctttcttc acggcgtccg cgatgccggg
    16921 aggcgcgtcg gcctgcgcgc gtgcgtcgac gagcgccggg atgtaatcgt ccgtgcattc
    16981 gcgagcgcga gtcatcgtcg cgacgcagac agccttcgga tcatctgccg tgctggcttg
    17041 cacggtggtg ggcgcgggct tggcctgcgc ggtatccgac gcgcaaccgg cgagaggagc
    17101 gagagcgaga gcaagagcga gggcagcaat cgatcgaatc atgggttctc ctttggcata
    17161 cgacgaactc cgaacgcgac gaggatccac gtgagcacca cgaacgaacc accgagcatc
    17221 acgttcgtca tcgtctccgc gaaccccatc aagagatagg gcagcgggtc cttctgagct
    17281 tcgggcacat tgacgacgta cttcgccgtc gatgcgagcc cggccgcgac gccgatgatc
    17341 atgcagaacg tgatcgtcgt cgtcagcgca cggatgagcg acaggcgctg tgcatccgcg
    17401 ttgcgcgcga acttgatgcc cgtgaccagc atggcgatgc cgaacacggt cagcgggaac
    17461 atgttgaaac cgccagcacg gaagaaatcg agcatgtgaa ggcaagaacg ccccaccccg
    17521 ctccgcgtga cagccttttt agctttgttt ttccgcgtca cgctgggaca cgtgagacgt
    17581 tctctatcag cgtggatccc gacgagcggg cctggtgcga cgggctgcga cgcggcgaaa
    17641 ccgctgcgtt cgacgcggtg tttgccgcgt atcgtcgacg gctgtacgcc tacttcatgc
    17701 gcatgacgcg ccgccgagat ctcgcggagg acctcctgca ggagacgttc atgcggctcg
    17761 cgcaacacgc caagcagctc gccgccgaca cgcgcctcgc tgcctggctc ttcacggtcg
    17821 cgcactcgct cgtcgtgagc tggggccgcg cgcaagccgt tcgcaaagcg ctcgccggcg
    17881 acctccccga gcgcgagccc gccgacaccg ctcgctcacc gctcgaagcg ctcgccgatt
    17941 cccaaacgca gctcgcactc gagcgcgcat tcgccgagct accacccgcg tatcgcgagg
    18001 tcgcgttgct cgttggcatc gaaggcatgc agccttccga ggtcgccgcg atcctcggcg
    18061 ttcgccccga tgcggttcgc cagcgcctcg cacgcgcgcg cacccagctt gccgaatccc
    18121 tcggcgatca cgcacctgct tcatggagga cgcgatgagc aacgacgacg acgccaacaa
    18181 gctcgccgaa atcagtcgcg agctaccgcc gctcgacatc gacgacgcag cggcgcagcg
    18241 gatcgcgcaa cgcgcacgcg cctcggtcgg tcgcggtccg tcgccgacga ggtttatcga
    18301 gccggtgctc gttggcttgc tcgtgctgag cgtcctcgtg tggacgatcc tcaagctcat
    18361 cgaagtgctc ggctagaggt actctccgcg cgtggcttcg ctgtggcagc aaaaccttcg
    18421 catcacgtgg tggctgggtt gcggtgcggc gctgatcttt cttgtcgtcc taccggtcta
    18481 cgcatgctcg ggttgcgaca ttcgcaaacg atccgagaag cgtgcgtgtc gcgaaggcaa
    18541 cgtcgatgca tgcctcgccg tcggtcgcta ctacgaaggc aaatccgacg gcctgttcgc
    18601 aatggtcctg agcaacggcg ccacgtcgac ggaagcatac gcgcgcgcat gcaagctcgg
    18661 ctcggcgatt ggctgcgagc gcgctgcgtt catgtcgatg catgccgaca acgcgcgtga
    18721 ccctgcctac acgcatcgcg atgccgcaca tgacttcgca tctgcgtgcg tcgacggcat
    18781 cgacgaagcc tgtgcagagc tcgacgattt ctacggtgac ctgatcaatg gcacgctcgc
    18841 cgaggacgtc gcatcgcgct acttcctcga tcgatgccgc aaggacaacg tcggcgctgc
    18901 ttgctaccgg ttcggcatct tcatgaagac gccgcattcc aaaggcgagg tgaagcccaa
    18961 tcccgagctc gagcagcaga tgtacaaaga ggcctgcgcg aagggctaca agcccggctg
    19021 tagcgcgtcc cgctgagctg cggtccgccc agtcatcccg cgaagtgaca gctcgacgat
    19081 tgtttccgag ccgccgtcgc gaacgaagcg agcgatcgcc tcgcgttcga tttgcaggag
    19141 cgtctccacc ggcggatgcc aacacggccc gtctggcgag accgcggtcg ccgctgtgaa
    19201 tggcagcggg tcgccattcg tcgcgcccat gtccaccgcg agaaagcgaa gaccggcgac
    19261 gtcgcccagc gtggttcgca gctcgtgaaa cacttgttgc gaggcgagcg agattttcga
    19321 catgctgcgc cccggcccga ggccaacata gcggccatcg cgcgcggcac gttcgaggac
    19381 acgccgaaca gcccgctcgt aagggcagta cacgtacacg aggtgcaccg accacccagc
    19441 ttcttgcgcg cgcgccaagg tgcgcttcga tgtggggaga ttgctggacg tgcagtcgaa
    19501 gacgatgcgc gcgtcgggaa gtgctcgttc cagtagcccg cggatcaccg tggtcttgcc
    19561 ggagccaggt ccgcctgaca tcaagatcag tcgacgctcc gacggtggga cggagctcga
    19621 ggcgagcgtg cgctcgaggc gatccttcgc gtatgcggcg gcggcgttgc gcgtcgacgg
    19681 cgtgaagcac agacgttcgc tgacactctg gtacgagggc gagaggtgac gcgcatagtc
    19741 catcgagaga atgcgcccgc cctgcgtgaa tggcagcgca tcgaattgcg catcggcgtt
    19801 cgcggtgtcc tgaacgatgc ggatgagctc tgcctcgact cgttgctgct ccggttcgag
    19861 ccctggccgc aacgcgatcc cgaggtcgcg cgccttcccc atacccgcct ccaccgacgt
    19921 catgtatcgc ccatgttctc acgaaggatg cgcgggtcca agaccctcgt ctcaggtcac
    19981 tgcggacgac gccgcgaggc cgtttcctcg acggtaaccc gcagctccgc ggaagcgttc
    20041 atcagctcgc caaagcgctc gcgtttcagg ctgaggagaa cgcacggtgt caacgtacgg
    20101 atggaggccg agcgcggcgt gtcaccgagt agcgccatct caccgaagaa atcgccatca
    20161 ctcagcacag cgacccgccg atccgggaga accgcatcga catcgacgac ctcgaccttg
    20221 ccgcgtgcga gcacgtagaa cttgtcaccg aggtcaccct cttcgaagac agagtctttc
    20281 tcggggtaat gctctgtgat gagcgatccc gcgatcgtct cgagcattgg gtccgcgagg
    20341 tttgcgaaca atgggattgc acgcagacca tcggccgtca tgcgcgccgg ttggccatcg
    20401 cgggcgatct cgactccgct ctgcttgcgc catagctccg cgtagcggcc ggcgagagca
    20461 aggagctccc gatgcgtgcc ctgttcttcg agccgcccgc ggtcgaacac gtagatgcag
    20521 tcagcgttgc gagctgacga caggcgatgc gtgaccagtg ctaccgcgcg accggcgcgc
    20581 aaccgatcca ccgtcgcgtt gatcgccgcc tcggcaaccg gatcgagggc agacgtagcc
    20641 tcgtcgaaga gcaggatcgc cggctttcgc agcagcgcgc gtgcgatcgc aatccgttgc
    20701 cgctggccgc ccgagagctt gccaccgcct tcgccgacga acgtgtcgta gccgcgcggc
    20761 aagctcgcga tgtggtcgtg aatctccgct gccttcgcgg cagcgatgac ctcgtcgtcg
    20821 gtggagccga gcttaccgag gcggatgttg tcggcgatcg acatgtcgta gaggtacgtg
    20881 tcctgaaaca ccacgcccat ctgcgctcgc agcgaggctt gtgtgacctc gcggacatcc
    20941 acaccgtcga cggtcacctt cccgaggtgc gcgtcgtaga aacgaaggat catgttgagc
    21001 acggtgctct tgccagagcc gctcgcgccg acgaacgcga cggtccggtc catcggtacc
    21061 gtcaagctga cgcggtcgag tgtcgggcga tccggcgtgt ggctaaaggt cacatcctcg
    21121 agtcgaatct cgcgcgcaaa cggagcgagg gtcgcggcat gtggtgcgtc ggcgacgtcg
    21181 atcggctcgc ctaggagctc gtcgatcctc tgcatcgcgc ccgaggcgga gagcatcggt
    21241 ggcaacacct ttcgggtcag gtcataggcc tgcccgcaca cgaccgcatt gagcgtgacg
    21301 aacgcgacga gcgatccggt cgtgagcgtt ccgtgaaacg cgaggtatcc gccaaagccg
    21361 atcaccaaga tttgcacgag cagaacgccg aggctggccg acgcgccgac gagcttgttg
    21421 agaaagcttg cgcgaacgaa gcgtgagctc cacgtgccga gacgcgcatc gaagcgtcca
    21481 gccgcgtgat cctcgaggcc gtagacgcgt acgacggatt gggcgcgcaa cgcctcgttc
    21541 acgccgtcca ccacgcctgc gagcgccttt cgctccgcat agcgcgcctg cgctgatcgc
    21601 ggcgccgtga tgcgcgtccc caggacgacc aaagggattc cgagcagcat cacggtgctg
    21661 aggcgccagt cgaggacgaa cagcaaaggg atgttcaaga ggagcccgac gatcgcgaac
    21721 acgccgtcga cgagacgcga tagcgctgca cgttcgacgt cggtgacgtc gctcgaaaac
    21781 gtcgacatga tgtcacctga ttgcgcgccc acgaagaacg ccaccggcag gcgttgaagg
    21841 tggccgaaga gatcacgtcg caacgcgcgg ctgaccgcag cgccgacctt cgctgttgcg
    21901 taatcggcat agaccgctgc gacgacggag aatagatagc caacgaggag cccgccgagg
    21961 atcgcgaaca acagaacgct gtcgcgcgcg acgatcgctt tgtcgatcac gagcttgagg
    22021 ctcaggccga agaaggtgtt gtaggcctgg ccgacgagca gcgcgatgcc gatcgagaga
    22081 gccgtccgcc agtgagggcg ccagagccgc agcgcggcac ggaggacgcc gaggacaccg
    22141 atcgcggcac gcccgcgttg ttccgcggca gcccactcgg tctcgaggac cgcgaccgta
    22201 cggccgtaga tacgctcgaa gaccgcggtg ggggcggcgg cgtcatatcc agcgagcgcg
    22261 tcacgaaacc gggcgtgacc gtaatccttc acgaggaacg cgacgaagtc cgtgacggcc
    22321 tcgccgtacg tgggctggcc gcgcccagtc ccgtccacga tgctcgcaag tgcgagcttc
    22381 gtcgcgccgg gtcttcgttc gcgtcgagct ccgccgtcga gccgccgccg gatacagccg
    22441 aacaggccgt caaagagagg cgttggtcgg gccgccgccg gtcgacacaa cgcgctgcag
    22501 agctgctcta agagaacgcg ctcgacgacg tgctgaggcc gatcgccgtc gacctcgatc
    22561 cgctgctctg ccggtagcgt ggcctcgagc agcgtctgcg cttcgacgca gaacgcgtct
    22621 agacgttcgg cgagtgcacg tacctggctg gaccggagcc ctccatccgt ccagcgaaga
    22681 acaacgtgcg tcgaggttag ctcttgggcc atccgacagt tccagtgaat cacggtagac
    22741 cggtatgctg gagcggtgcg agcggtgatt gtcatcgtga tctgcctgcg agcttgggtc
    22801 gctgcagcgg atccggcagt gctcggacaa acaccgcgcg agcagggcat gaagctctac
    22861 tcgcaagcga cgaagatgtt cgaggaccag gacttcatcg ccgctgcggt gacgttcggc
    22921 caggcagcgc agctgttgtc gcgcgtcgac cgcgacgaca agggcggcgt gatcgacaag
    22981 gacgcgcaca cgttccgcag cgccgcgctc tcgaacaagg cgaccgcgta ttcgcgcgcg
    23041 ggcctctatg tcgaagcgtc cgccgcgttc gcggaggttc gcgaccagtt cgctgcggag
    23101 ctcgccgaga aagagaagac cgagatcgcg gatgcgatcg cgcggatcga ggagcggatt
    23161 ggtacggtag cgttgaaggg cctaccggac gaggagctcg aagttcgatt cgacgggcgc
    23221 ttcgagcgac ggccgctcga caagccgctg cgcatgagcg aaggcgcgca ctcgctcgag
    23281 ctcaaagcga ccggttacaa gccctacgtt gctgaggtcg aagtggtggg caaccaagag
    23341 gtcgtcctcg agctgaagcc cgtaccgctc gcgacgcccg cgaagctgcg cgtcgaatcc
    23401 acgatcgacc gcacaagtgt cgtgatcgat ggcaagccga gcggtatcgc gcccgtcgag
    23461 gtttcggtga agccggggac gcatcgagtg cgcgtgagcg cggagtcgta tgtcgcgcag
    23521 gaaagcgatg tcgcggtgaa gcctggcgag cgcgcgatca tgcggagcgg gttgattcgt
    23581 gcgcgcgcac cgctgggcct gcgcatccag cccgagtttc tgccgatgtt tgcaggccgc
    23641 gccgacacgc cgttcggcaa ataccaaccc gcgatcgggc tgtcgctgtt ccacgatgcg
    23701 cttcgctatc gcacgctgcg ctttggcatc gaggtcgtgt actcgccacg cgcgctgaac
    23761 tccgtcgcga ctgggatgat ccagacgtgg tgccccgatc gtacggcgtg gaaaccgatc
    23821 gcgtggtgtc cggtcaacgc gacggcgagc tacgtgtttg gcggtgaaga aggagacttc
    23881 gcgtcggggc aagcgcgagg ccgtggcttc accgcgttcg agtaccgccg gggcgctggt
    23941 ttcgttcgcg caggtgtcgg gctcgtcctc gaggactatc gcaaatacgt cgtcgagcag
    24001 accggcgagc ggttgtacga gcgcttcctc tttctgtggt ccgcgtcgta cgagctctcg
    24061 gtggggctgg acctgtgaag cgcctcgcag tgctcgcgct gctgtttgcg ttggagaacg
    24121 cgcacgccga ggacatcgac gatccacgcg tcttgtttgc cgaggggcgc ttcgccgcgg
    24181 ccgcgcaggt gttcgagcga cggtggcaag ccaaaggcga tgcgaccgac ggcgtgaacg
    24241 cggtcgttgc gtggcgaacg gctggtaggt acgcgcgagc cctcacgctg ctgtcgcgcg
    24301 tgcgctcggg caagcaagca ccgacgggtg ccgctgccac gacggccgcc gacctcgacg
    24361 agcgactcgg gacgctcacc gcgatggcgc ggatcgaagg caagctcgat ccgaatgcgg
    24421 tgattcgcgt cgatggcgaa ccggccgagc gctttaagga catgctggtc ctcgacgtgg
    24481 gtgagcacga tatcgagatc gagcagccgg actgcgatcg gtttctgcag caagtcaccg
    24541 catacccgag cggtaagctc gtcgtcgcgt tcaagccacg atgcgacgtg atgggcacac
    24601 tgcatgtcgt gctcgcgacc gatccagggg cgtcgttcac ggtagacggc gtcgagcaca
    24661 cgacgacggc gaacgaggcc gatgtgcgat tgccggccgg cgagcatcag ctttctctgg
    24721 ccgcgctcga acgtcccgtc tacgacgaga ccgtcgtcgt tcgcgagaaa gagacgacgt
    24781 cgctgcgcat ccgatatcca tggcgcgcac gcaagctgag cttcatcctc ggcgtcacga
    24841 acgaggtccg cgcgggacag ttcatggtcg ggacggcact cggcctcacg ctcggtgtgt
    24901 ggacgcccag ctttcacgcg acgttcgaca tcggcgcgat ggtcagcaac gcaaagggtc
    24961 tcgagcccta ttacggcgcg tggggacatc cgtggctcgc cgacacgctc gtcattcaca
    25021 cgccgcgtgc gccggtctgg cacggcaagc tcggaccgta caggctcgcg ttcgatccgc
    25081 agccgctcgg gattcgattc gacgagattc gcgcattttc atatgcgggt attcgcacgt
    25141 gcgaggaggc cgagccgcgt gttcgcgcgt ggagtgtgct gccgctcgcg ttgtctgcgg
    25201 acggaccgta cgcacacttc gagctgacgc tgtggccggc gagcgtgctc tggtatcacc
    25261 ctgcggcggc gctgtgcggc gacgacgatc tgccgagcga catcggcttt ggcgcattcg
    25321 tcacgctgac cggcgggtgg cggttgttct agaagatgtt ctgcgtcggc cggcacagcg
    25381 acgcactcgg atcccagcag acggtggcca ggtacggcgt cgcgtcctgc gcgatctccc
    25441 aggtgccgag ctgaaacgat tgttcgacgc tccacgcatg cgcgcccgcg gtgaacggca
    25501 cagcgctaat cggcactgcg agctcgatgc ccgtcggccc caccgcagcg cggaccggga
    25561 cacccgtcac cgacaccgtg tagccgacga cggtctcgac gccgccgggg gtcacgagaa
    25621 attcgagcgc gtacggcgga agcacgagcg gtgtgagctg gaagtcgtag cggtgacgtg
    25681 tatccattac cggcgcaccg acggttggcg cgaagatcgc gatctctccg ttcgtcgtct
    25741 cggtgacgta cacgccggtg acctcgccag ggcgacagtc ggtgcaatcg ggtggataga
    25801 ggagcagcgg cgcccagtcg ccgagatcgc cgtcgatctg gatcgcggca ggcgcaaagt
    25861 cgcacgtccc gtcccacgtc gtgtgtgcac gattttgatt gcagatgtag ccggggtcgc
    25921 tgcagcccgc taaggcgaat gcgatgacga cacgcttcat cgcgtcacct cgcacgctat
    25981 cgacggcagc gcatcgagcc ccgatcgata gccgaagcaa acgtcgatca tctcgccgcg
    26041 cagcacttcg ccaccgcccc gcgagccaac cacgcgcatg cgcaacgcgc cctgatacgt
    26101 cagccaagag ccaatgatgc gtgcctcgaa gccgtcggat gtcgacgcga gctgcagcgg
    26161 cggctggtcg ggcgtcagca ccatgtcgtt cttctcgtag cgaataccgg ccgcatcgat
    26221 ggtcaggcga tcgctgccac cgctcgcagg acgcagcgat gaaggagcaa gctcgatcga
    26281 aattgtatcg agtggtccac cggcgaagac ggcacgcatc aagacgtcgc tctcttcgtc
    26341 gagattgctc gcgaacgcga gacgcgcgcc ggtgatgtcg agctccggga tgccggtcca
    26401 atcggcggtc tgtccatcga cgcggatcga tggcgccatc gtcgtcatgc atggtgcgct
    26461 gttcgtggcg cgcgagtgtc cgggcgcgca ctccttcacc ggtggattcg gatccggatc
    26521 gctgccgatg ccgaaatcgc acgcggcgag tacgaccagc aggcttgcga gcctcgtcat
    26581 gatcccggct tgggaccgac gagctgcttc gtccgatcga ttttctcttc gggcgacttc
    26641 gtctcgaccg gtggcttcgt gatgtccgga acggccgagc ctgcgccgct gccagccttt
    26701 ggcgtcgtcg cgattttcgc ggtcgttttc ttcggcgtct tcttcggcac ggtcgtctcg
    26761 gtgggtactg ccgcaattgc cgacgcagag cccactgcag agccggcgcc ggcgggttcg
    26821 acaacaggcg cggcgaccgg cggtgtcgga gccgatggtg caggagccgc cgtctgcgga
    26881 ggatcggctg gacgcgctgc aggagctgac gacttcgact ccggcgtgtg cttgttcgta
    26941 accacgacgc cgactccgac gacgatcgcg agcgcaccgc cggccgcggc gatcatcggc
    27001 cagcgcgggc gtcgcttcgg cgcggtcgag acctcggcgg ccgcagcggt cagtgatgac
    27061 tgtccactcc acatcggcgc ggtcgtggcg ccgagtccag gcgcactcgt ctcggcgagt
    27121 gggttcacgt cgggccgagg accgctctcc atcgtctcgg tgagcgcgat cgatgcattc
    27181 cccgacgcga gcggcgattg cgatgcggcg accgactgcg ccgtctcgac gggaagtccg
    27241 acggcttcga acagctcgcg gcgaagcgtc gccatgtccg gtggccgctt gctcgcgtct
    27301 ttgtcgagac accgcgcgac gagagcgacg agcgccggcg ggacatcctt cgccagcttg
    27361 tcgatgcgcg gcggcatctc ctgaacgatc tggatcagca tcatcgtcag gctctccgcg
    27421 gcaaacggga gctgcccggt gagcatgtga aacaacacga cgccgagcgc gtagatatcg
    27481 gcggccgggc cgaccttctc gcctcgcgct tgctcgggag ccatatacgc cggcgtaccg
    27541 atgagcgcgc cactctgcgt cgcgccgacg ccaccgagct cgggacgcag tctcgccatg
    27601 ccgaagtcga ggaccttcac gtaatccgct gaccccgcgc gcttgatcaa gaacacgttg
    27661 tcgggcttga ggtcgcggtg gacgatgccc ttggtgtgcg cagctgccag agcgtctgca
    27721 atctgcgcag cgatcttcgc ggcgcgcacg gccggcatct gcttggcctg acggatggca
    27781 accgagagcg tcttgccctc gagcagctcc atcacgatgt agctcgtgcc gccctcgttc
    27841 acgaggtcgg tgcactcgac gatgttctcg tgccggatga tgttgacggc gcgcgcctcg
    27901 gcgaagaacc gctcggtcat cgcgcgatcg gcggcgacct gcggcttgag cttcttgatc
    27961 gcgacctgcc ggccaagcgc ttcgtgcacg gcgagaaaga cctcgcccat cgcgccttca
    28021 ccgagctgtc tctcgatgcg atacgccccg agtcgagtgc cctccagcgt cgatgcctcg
    28081 ctcatacccc aagggcgatc ttagcacgcc gccggttagc cactggcgac gttcgccgtt
    28141 cgcaagcggc gcacgaggac acgcagcaca cctgcggcta cctcgggcct ttcggcaagg
    28201 acatcgctga aatcgtcctg gccgaggcgt acggctacca cgggtgctgt cgccaccgca
    28261 gtcgcgctgc gcggtgcggg atcgagaatc gccatctcgc cgaacacttc acgcgtcccg
    28321 agctcggcca gcgttcgctc tcccgcggcg atgcgcacct tgccctcgac gacgagatag
    28381 agcgagtcgc caggatcgcc ggcggcaaag atcacttcgc cttcttcgaa cgacacttcc
    28441 tccgctgcag ttgcgatggc ggcaacatcc tcggccggga tcgcggcaaa cagctcggcg
    28501 cctttgagga acaagacttt ctcggcggtg gtgagcatct tcttggtctc cggtgtcctg
    28561 tcgatagaag ccaacgcatg acgtgcggtc tctgcgacca acggatttgg atcggtcgtg
    28621 agcttttcga ttcgcgggcg cgcgccctga tcgcgagctg cacccgcgta gtacgccgtg
    28681 catgcgcgaa tccatggatt gcgatccgcg agcaggttgt cgatccaacc cgcgcggtcg
    28741 tagcgaggga gccgcagctt cttgctcgca gcggcgagct tcgcgtcccg cgacgaatcg
    28801 tcgacgatcg cgagcgcgag cggcttgagc tctgcgacgg agcgcggcca ggagcgcgca
    28861 tcgagaagct cgaccgcgtt ggcacggcgc gtgggatcgg ggtcgcgaag gttcgcacgg
    28921 acggtcgcaa tctcgagctc cggctcgagc gcagcgagca agtcgaacac gcggtcgcga
    28981 gcacgctcgc ttcgttcacg aagagcgcgc gagaggagcg cggccggtcc ggcaagatca
    29041 tcggtgacga acggcacttc gatgcgcgtt ccgtccggct tcatgatcga ttcggcgtga
    29101 ccgagcccag ccgcggcagc catcgcgaca aacgcgcggg agatctcggt catgcaagcg
    29161 tcgacgatgg cggcaggtgg cggcgagcga tcggggtggt cgcggcgcag gcgtgcgagc
    29221 gcggcagcgg ctgctgctcg ccgaacctcg cgcttgtctt gcaggatcga cacgaggcga
    29281 tcgacggcgt ctggcgcagc gatcatgccg acgatacgaa tggcttccgc agcgaccgcg
    29341 tcgtcgtcta cgtcgagcag cggatgcaca tgcgcgagag ccggctcgcc gatcgcgacg
    29401 agcgcacgga tggcatgacg cgcatgacgt gagccgaggt gcacgcggac gggctcggcg
    29461 agacgcacgt ctgcgatttg ttccgcgatc gcgaagccga gtgcctgctt gacggggtcg
    29521 gcgagccacg cgcgcgcacg ctccaccatc tcggttcgtc gatcgagatc gccgcgccga
    29581 tacatcgtgg ccgccgcact cgctgccatc gtcggatcag cgccatcgag cgctgcggcg
    29641 agctcggtgt cggtgagacc gcccgcgaca tccgcgagac acgcgaacgc atctccgcgg
    29701 accgccgcat ccgagtgagt gaggaggtcg cgcaactcgt cccgcacccc cgccggatcg
    29761 agccgcgcga gatcgatgcc gcgacgaaca accgccgcat ccgtcgagct gagcgcttgc
    29821 ttgatcgcgc gcgacgcctc cgctgcgagc cccgtatcgc cctcctcgtc cggcgccgga
    29881 atccggcggc gacgcaacgt gtccgcgacc gacttcacgt acgcgccgcg catgcgcagg
    29941 accagcgcga tccacgcggt gatcgcgacg atggcagcga tcgcgagcgg cgttcgcgtc
    30001 gtgagatagc tcttgtacgc gagcaaggcg acgccgacgc ttccttcggt gccgggcttg
    30061 atcagcatgt cgatgcgtcg cttgcggcga cttcgctcgg ccggaggcgc gggcaagtag
    30121 agcagctgtt gccccgacga atcgacgctg ctgcggaacg tcgtgtccgc ggccttgctc
    30181 gcggtagcag tgaacagaga cggcgcgagc agaagcgcga tcgctccaag accgagcgtg
    30241 accggcaaca gcaggagcgc gccggcgacg ccgaatcgcg cgaggatctt gcgcgtgaac
    30301 aacaactgaa tgacgagcgc cgcggccgcg gtcacgacgt agagcaagcc gaagaagcgg
    30361 accatcccga cgcggtcccc tgcatatgca gcgttcgcga tcgacttgaa ctggaagtcg
    30421 atcagcgtca cgccgagcat gccgaacatc aacagcgggc cgagcagatc gagatgccgg
    30481 cttccggcgg gttggccact cgtttgctgg acgcgcgggc gggcggcaac ctccgtcggc
    30541 gggcggcgac cgagcactgt cgctaggacg gcgacgacga tctgaaacgc tgcgcagcca
    30601 aacaggaggt tctcggcgcc tgtgcgcgac acgatcgcgc tgacgaccaa gccgacggtg
    30661 atcgtcgcag cggtcccact tgcagcgatg atcgtgaata ccttcttcgc ctcgcgcgcg
    30721 ctgaaccgtt cacccgccgc gagccagaac tgcatgctga gcaggacggc acagatatcg
    30781 aggccggcat acagcacgta gtagagccag tgcggcccga gcgcgaaccg cgcggcgacg
    30841 agcaagacgg cggcaccaac cgcggtgacc ggcgcgacga tcttcggcgg cattcgatcg
//
LOCUS       DATLBZ010000430        12386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3259414, whole genome shotgun sequence.
ACCESSION   DATLBZ010000430 DATLBZ010000000
VERSION     DATLBZ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12386
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3259414"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VMZ53_21820"
     CDS             <1..494
                     /locus_tag="VMZ53_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31170.1"
                     /translation="TPVPGASDKDAPSRATPVPGASDKDAPSRATPVPGAAGKDAPSR
                     ATPVPGATGKDAPSRATPVPSANGRPTPVPAAVTAASGAPIPRPAPGPSPAALATLEA
                     AAPDAMSRQVSRIPGTPRPAAAGPQIGAALLQRQATSKLPWVLVATLCVAVLSLVAYI
                     VLT"
     gene            491..1624
                     /locus_tag="VMZ53_21825"
     CDS             491..1624
                     /locus_tag="VMZ53_21825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31171.1"
                     /translation="MKRFAVAALALLACSSHDKPKPQQEPERPRRVIEPPSRGVRALP
                     PHAIRADGVGPYKLGATVSELLDQLPSGPRITQLTIPGIARRDLLRAEDDAILIGVEP
                     QGKATFVAVVRGEIARTEAGIHVGSTRDELTHALGAPLEDLDHARDSHIVVPTGLKSA
                     RVVMEGDRIAAIVIAAEPVRARTDEAASDAATEAPCTRPPDDRSKGLIGVCLTNAGEF
                     LRTQDDEIVLVARDGEKPVARMPVPGLVFAAALRNPLDNRDDIVAIARSDDAASRSWS
                     LIAFRLAEGKLVVTIPPKLLYQLTAANARWIGADLHDLDLYLELSSRSDAIEVGGLLT
                     TRISDQIRDIVVISTVPVSRTRSKAPTHEAIDAGTSEKPDVGP"
     gene            1717..3549
                     /locus_tag="VMZ53_21830"
     CDS             1717..3549
                     /locus_tag="VMZ53_21830"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HUS31172.1"
                     /translation="MKFLCDKCKTRYSIGEDRVRGKILKIRCKNCANVITVREGMDGA
                     EVPGDRPRTPTGGQPAVSAAPAGGGALGAAFQSQMASSKPPPALEEEWYVSIDGVQSG
                     PLSLTDAQKWVSAKPFDAELHCWSEGFDDWLPVDKVSHFRGLRKAPPPMPSLPPKRPS
                     GKTVPPPIPEKPLFAATMASIEKSASQPNLPTLTSPSTKPGMPAVPAITSQSPGSGGI
                     KTNGKPVVGQQPLSAGAAALAQAFAPDPSDSMTSVGSPAFESEPIPPASGPNDPFFNA
                     VAAAKNDPFAERDPFAAKGEAKKPVVPIHNDDDDNLEIGEVSRVVNLKDLAQKPKPIA
                     KTPTAGFDRISGTGSVPRIGGGTASNPRVSASNARIGGLTGSSPKLSPSELGMNVDPA
                     LASQLTGAAGEPHASESVVAQSFKERHRRGLMALIGVSAIMVVGVVLLFVFVVNNNNE
                     TYEGGLGGTHQIDTSRPEDIVRKQLPPVPNAGSNAVIANNATKKWLPRNNNLTTNNGA
                     QEEVGGNALKASEVEDMAAKQGEGTKRCYMRAQKGALGFEIQDVKKIAVTLSVNKDGS
                     VNSVQLSEHANDTFGQCLIARIKAWKFRESGGGTFRISLAFSNS"
     gene            complement(3550..4689)
                     /locus_tag="VMZ53_21835"
     CDS             complement(3550..4689)
                     /locus_tag="VMZ53_21835"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HUS31173.1"
                     /translation="MPPIYLDNAASTRVSDDVLALMTEVMRTAWGNPSSSHPQGAAAR
                     SHIDTARGKLLAALGDRDAKHGDVVWTSGCSESNALAVFGAAQTHAGSIVISTLEHPA
                     VSTLADKLATDGRAVHRVAPAAGCVLDPDELAAVAKDAGVVSLIMVQNEIGVVEPIAA
                     LAAAIRTASPGAHIHVDAAQALGKVALDVATLGVDSISIAGHKLHGPKGTGALWLAKS
                     ARISPLWVGGGQQRGLRGGTQDAPGAAGLGLAAATAVTSLATARTRWLEFAGRVTSHL
                     TDRGVQFRQLVPDFRRAPHILALGIAGVPASALRTVLSSRGVYISTGSACADGDSKAS
                     PVLESIGLPQDHGMVRLSFALDTTQADVDEAARILADVALELRKR"
     gene            4688..5800
                     /locus_tag="VMZ53_21840"
     CDS             4688..5800
                     /locus_tag="VMZ53_21840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HUS31174.1"
                     /translation="MSDLGVYLHYPWCRKLCPYCDFAVEVGAPEHDAYLAGVLRELEA
                     RAGQFSGELVSIYLGGGTPSIWDPACVGTAIAAIRAKFGEAALREVTLEANPTDCTSE
                     RLAAWRDAGINRLSIGVQSLAQEELVTLGRDHRFGDGMAAIERVLAFGGFTVSADFIL
                     GVPPRRMPDWAAVIALPIDHLSVYELTIEDRTAFGQRVKSGRLVPLDEEALMELYVAT
                     HRTLTAAGFEHYEISSYARPGKRAVHNSLYWSGAPFLGLGVGAASLALHADGSGERAT
                     NPRKARDYLAAPGVAAEVTAIPAAEMATDRAWLGMRTVDGLVEPALPAEVVRWLLDEK
                     LAERRQLPGTGVYRICPTLRGFLMADRIAARIVEAL"
     gene            5816..6859
                     /gene="hrcA"
                     /locus_tag="VMZ53_21845"
     CDS             5816..6859
                     /gene="hrcA"
                     /locus_tag="VMZ53_21845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HUS31175.1"
                     /translation="MGGNELSRRAQKILQAVVTEYLQGGDAVGSRTVTRRHDLGLSPA
                     TVRNVMADLEEMGLLEQRHTSAGRVPTTSGLRLYLDSLLKVRGLNPREKDEIRERITA
                     GTPDEVMQRASRMLADLTHHAAVILAPDPDQTRLERIEFIPLRDGKLIAVLVTTDGRI
                     ENRLLLEDVEPSRLERIHNYLNHLLSGMTIDEVRERVIRELGEDKNQYDEAVATALRL
                     GHALFASQPKQDVVVTGHANLIDASEDSQPDRIRELLRTLEDKEVLVRLLDRTRQAEG
                     LQVFLGAETAMQALSSSSVVAMPYGPDEQPIGALAVIGPMRMNYGKVMSVVDYTADTV
                     SQLLAELGRGSNS"
     gene            6881..7510
                     /gene="grpE"
                     /locus_tag="VMZ53_21850"
     CDS             6881..7510
                     /gene="grpE"
                     /locus_tag="VMZ53_21850"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HUS31176.1"
                     /translation="MSGDASQDDTKPASEEATKGDGDVLPVEVEAPDAKPEEAKEAAK
                     PDPVAELEAKLAAVEKEKKDNWEKYLRSAADIENMRKRQKRELEDAKTESKFKVLKEM
                     LPVVDNLERAVEHATQGGDVDPKLKGFVEGVQLVLRQFSNAFERLEVQSVDAMDQPFD
                     PNQHEAISQQESDKAPGTVVQVLQRGYKSGDRLLRPALVVVAKAKAQTE"
     gene            7507..9351
                     /gene="dnaK"
                     /locus_tag="VMZ53_21855"
     CDS             7507..9351
                     /gene="dnaK"
                     /locus_tag="VMZ53_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HUS31177.1"
                     /translation="MSRVVGIDLGTTNSCVAIMDGDQPVVIANAEGTRTTPSVVGFAP
                     SGERLVGQAARRQAMTNAENTIYAVKRLMGRKYEDSEVQRHVLTCPYEVVGAPNGDAH
                     VRVRGRDYSPPEVSAIILQKMRQTAEDWLGEEVTEAVVTVPAYFDDAQRQATKDAGRI
                     AGLNVLRIVNEPTAAALAYGVEQQGASKVAVYDLGGGTFDISILELSDGVFRVRSTAG
                     DTFLGGEDFDNAIVEHMLAAFMEQNDGMDLRGDRMALQRLKDAAERAKIELSSSMVTE
                     INLPFLAAGTDGQPRHLIMQMLRDDLETLVEPLVQATLEPCRRALEDAGLSPIEIDAV
                     ILVGGQTRMPRVHTVVAEMFGKEASRWVNPDEVVAVGAAVQAGVLTGEVQEVLLLDVT
                     PLSLGIETMGGVFTRLIDRNTSLPARATEVFSTTVDNQPFVNVHVLQGEREMAADNKS
                     LAQFELTGIPPAPRGVPKIEVAFDIDADGVLAVSARDMVTGRSHVVTVQPTTGLSDHD
                     IERLVAESETMQNDDAVRRLIVEARNKAEGLFYSSDKAMAEFGQMLAPEEFDFLTTEL
                     AECRTALQTEDLPTVEDAVARLEVAAQRIGEAIYAAADGGGSDIGGGM"
     gene            9363..10265
                     /locus_tag="VMZ53_21860"
     CDS             9363..10265
                     /locus_tag="VMZ53_21860"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HUS31178.1"
                     /translation="MAKRKRDYYEVLGVDRSATEGDVKRAFRELARQHHPDVNPPDAG
                     ERFREINEAYAVLSDKDQRARYDRWGHPDDGGSGLGAVVDAAQEMIRDVLRRGRSKPK
                     GRDLRYTLEVTFEEAAFGATKTIQIPGADGKPRDFSVVVPASTKDGTVKQFRGEGEPS
                     RAGGAAGDLHVIVRIAEHTAFRRDGTDVHSDFTIQFTQAALGAVIDLPTLDGPVKMRI
                     PEGTQPGRVFRIRGRGIPQAPGKNAPRGDHLVHINIEVPTELTPRQKQLIEELAKESG
                     TSDVAPGKPGQKTLLGRVRSLLEE"
     gene            10802..12343
                     /locus_tag="VMZ53_21865"
     CDS             10802..12343
                     /locus_tag="VMZ53_21865"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HUS31179.1"
                     /translation="MTGCGPKVPRGTWVEHEELGRAHSSTNGGSGATSAGSATAKPIA
                     IDLAKLDAATIDQLDYATCVAVIEQLGDRKPAARVALRAARLAHHQGDDVDARALIAR
                     ATNAADEGEVHAELAALAAEAAETPVDRKVIAVLLPLSGRFAALGSELRVAIELAPPE
                     GVSWLFVDTKGEPDGAVAAVESAWKKGAVGILGPVGTREATAAARAAALHGMPIGLLA
                     PADGADAASGVFRLVGSPADEGRAVARIAQADSFATVAVFAPRDDVGQETADAFAAEA
                     VRLGLRVTKTANYDPTDGNLEPDVKQLLDLVPARNPRLAEHLAKNPKNGWKTFSPDVP
                     FSLLYIPDRYDRAAIVAAFLPYYGVELRTAENMNSAQLQRKHGGHVPTVVQLVGGAGW
                     HHPSLPVRGGEAVQGALIVDAFAGGDGGEIAADFVAAFQQKTNRTPSAAAAEAFDAAT
                     LMAKARAAVGMTSPDPRGALRTAIARAKLDDGACGPASMDGDGELAREPALLEVQADQ
                     IVLVP"
BASE COUNT         2140 a         4076 c         4294 g         1876 t
ORIGIN      
        1 cgacgcccgt gccaggggcg agcgataaag acgcgccgtc acgcgcgacg ccggtgcccg
       61 gggcgagcga taaagacgcg ccgtcacgcg cgacgccggt gcccggagcg gccggaaaag
      121 acgcgccgtc acgcgcgacg ccggtgcccg gagcgaccgg aaaagacgcg ccgtcacgcg
      181 cgacgccggt gccgtcggcg aacggtcgtc cgacgccggt gccggcggcc gtcaccgcgg
      241 cgagcggtgc tcctattcct cgccccgcgc cggggccgtc gcccgccgcc ctcgcgacgc
      301 tcgaagctgc ggcgcccgac gcgatgtcgc gacaggtctc gcgcattcca ggcacgccgc
      361 gtccggcagc cgcgggcccg cagatcggcg ccgcactctt gcagcggcag gcgacgagca
      421 agctgccgtg ggtattggtc gcgacactct gcgtggccgt tctgtcgctc gtggcgtata
      481 tcgttcttac gtgaagcggt tcgcggttgc agctctcgct cttctggcgt gctcgtcgca
      541 cgacaagccg aagccgcaac aggagcccga gcggcctcgt cgcgtgatcg agccgccttc
      601 gcgtggcgtg cgcgcgctgc ctccgcatgc gattcgagcc gacggcgttg gaccgtacaa
      661 gctcggcgcg acggtgagcg agctactcga tcagctgccg tcggggccgc gcatcacgca
      721 gctgacgatt ccagggatcg cgcgccgcga cctcctgcga gcggaagacg acgcgatctt
      781 gatcggcgtc gagccgcagg gcaaagcgac gttcgtggcg gtcgtgcgcg gcgagattgc
      841 gcgcaccgag gcaggcattc acgtcggctc gacgcgcgac gagctcacgc atgcgctcgg
      901 cgctccactc gaggatctcg atcatgcgcg tgattcgcac atcgtcgtgc cgacggggct
      961 caagagcgcg cgcgtcgtca tggaaggcga ccgcatcgcg gcgatcgtga tcgcggccga
     1021 gcccgtgcgc gcgcggaccg acgaagcggc gagcgatgcg gcgaccgagg cgccgtgcac
     1081 gcgtccgccc gacgatcgca gcaagggcct catcggcgtg tgcctcacga acgccggcga
     1141 gtttctccgc acgcaagacg acgagatcgt gctcgtcgct cgcgatggcg aaaagccggt
     1201 ggcgcggatg ccggtgccag ggctcgtgtt cgcggccgcc ttgcgcaatc cactcgacaa
     1261 ccgcgacgac atcgttgcga tcgcacgcag cgatgatgcc gcgtcgcgca gctggtcgct
     1321 gatcgcgttc cggctcgccg agggcaagct cgtcgtcacc atcccgccga agctgctcta
     1381 ccagctcacc gccgcaaacg cccgctggat cggcgccgac ctccacgatc tcgacctcta
     1441 tctggagctt tcgagccgga gtgacgcgat cgaggtcgga ggtctcctca ccacacgaat
     1501 ctccgaccag atccgcgaca tcgtcgtgat ctcgacggtt ccggtgtccc ggacccgttc
     1561 caaggcgcct acgcacgaag ccatcgatgc ggggacctcg gagaagcctg acgtcgggcc
     1621 ctgaaccctt tgcgagcact ggattttatt ggcacaatcg aagctcgtgt cgcgcactgt
     1681 tcagggatgg cttcgtgacg tcacgaggag cgtggcgtga agtttctctg cgacaagtgc
     1741 aagacccgct actcgatcgg cgaagaccgc gtccgcggca agatcctcaa gatccgctgt
     1801 aaaaactgcg cgaacgtcat cacggtccgc gagggcatgg atggcgcgga ggttcccggc
     1861 gatcggccgc gcacgccgac cggtggccag cccgccgtct cggccgcacc cgcaggtggt
     1921 ggcgcgctcg gtgccgcgtt ccagtcgcag atggcctcgt cgaagccgcc gccggcgctc
     1981 gaagaggaat ggtacgtctc gatcgacggc gtgcagagcg gcccgctctc gctgaccgac
     2041 gcgcagaagt gggtctccgc aaagccgttt gacgccgagc tgcactgctg gagcgaaggc
     2101 ttcgacgact ggctgccggt cgacaaggtc agccacttcc gcggcctgcg caaagcgccg
     2161 ccgccgatgc cgtcactgcc gccgaagcgc ccgagcggca agaccgtccc gccgcctatt
     2221 ccggagaagc cgctgttcgc cgcgacgatg gcgtcgatcg aaaagtcggc gagccaaccg
     2281 aacctcccga cgctgacgag cccgtcgacg aaacccggca tgccggccgt tcccgcgatc
     2341 acctcgcaat cgcccggctc cggcggcatc aagacaaacg gcaagcccgt cgtcggtcag
     2401 caaccgctgt ccgccggtgc tgccgcgctc gcgcaagcat tcgcacctga cccctccgat
     2461 tcgatgacga gcgtcggttc gcccgcattc gagagcgagc cgatcccgcc ggcgtccggg
     2521 cccaacgatc cgttcttcaa cgcggttgct gccgcgaaga acgatccgtt cgccgagcgc
     2581 gatccgttcg cggccaaagg cgaagcgaaa aagcccgtcg tcccgatcca caacgacgac
     2641 gatgacaatc tcgagatcgg tgaggtctcg cgcgtcgtca atctcaaaga cctcgcgcag
     2701 aagcccaagc cgatcgctaa gacgccgacg gcgggcttcg accgcatcag cggcaccggc
     2761 tcggtcccgc gcatcggtgg cggcacggcg tcgaatccgc gcgtctccgc ctccaacgcg
     2821 cgcatcggcg gcctgacggg cagctcgcca aagctgtcgc cgagcgagct cggcatgaac
     2881 gtcgatcccg cgctcgcatc gcagctcacc ggcgcggcgg gcgaaccgca tgcgagtgaa
     2941 tccgtcgtcg cacagtcgtt caaagagcgt catcgtcgcg gcctgatggc cctcattggc
     3001 gtctcggcga tcatggtcgt cggcgtcgtc ctgctgttcg tcttcgtcgt caacaacaac
     3061 aacgagacgt acgagggcgg cctcggcggc acgcaccaaa tcgatacgtc gcggcccgaa
     3121 gacatcgtcc gcaagcagct gccccccgtc ccgaatgccg gctcgaacgc cgtcatcgcg
     3181 aacaatgcga cgaagaagtg gctgccgcgc aacaacaacc tgacgacgaa caacggcgcg
     3241 caggaagaag tcggcggcaa cgcgctcaaa gcctccgagg tcgaggacat ggcggccaag
     3301 caaggcgagg gcacgaagcg ctgctatatg cgcgcgcaaa agggtgcgct cggcttcgag
     3361 atccaagacg tcaagaagat cgcggtcacg ctgtcggtca acaaagacgg cagcgtcaac
     3421 agcgtgcagc tctccgagca cgcgaacgac acgttcggtc agtgcctgat cgcgcgcatc
     3481 aaagcgtgga agttccgcga gtcgggcggc ggcacgttcc gcatctcgct cgcgttctcg
     3541 aactcctagc tacctcttgc ggagctcgag cgcgacatcc gcgaggatgc gtgcggcttc
     3601 gtcgacatcc gcttgcgtcg tgtcgagcgc gaacgacagg cgcaccatgc cgtgatcttg
     3661 cggcagaccg atcgattcga gcaccggcga cgctttcgag tcaccgtcgg cgcaggccga
     3721 gcccgtcgag atgtagacac cgcgcgacga cagcacggtg cgcagcgccg aggcggggac
     3781 gcctgcgatg ccgagcgcga ggatgtgcgg cgcgcggcgg aagtcgggga cgagctggcg
     3841 gaactgcacg ccgcgatcgg tgaggtgcga cgtgacgcgg cccgcgaatt cgagccagcg
     3901 tgtgcgtgct gtggcgagcg acgtgaccgc agtggcggcg gcgaggccga ggccggcggc
     3961 gccgggcgcg tcttgcgtgc cgccgcgaag cccgcgttgt tggccaccgc cgacccacag
     4021 cggcgagatg cgcgcggact tcgccagcca gagcgcgccg gtgcctttcg ggccgtgcag
     4081 cttgtggccc gcgatcgaga tcgaatcgac gccgagcgtg gcgacatcga gcgcgacttt
     4141 gccgagcgct tgcgccgcgt cgacgtgaat gtgcgcgccc ggtgaggcag tgcggatcgc
     4201 agcggcgagc gcggcgatcg gctcgacgac gccgatctcg ttttgcacca tgatcagcga
     4261 gaccacgccc gcgtccttgg cgacggcggc gagctcgtcg ggatcgagca cgcagcctgc
     4321 ggccggggca acgcgatgga ccgcgcgacc gtcggtggcg agcttgtcgg cgagtgtcga
     4381 caccgcaggg tgctcgagcg tcgagatcac gatcgagccg gcatgcgtct gcgccgcgcc
     4441 gaacacggcg agtgcgttcg attccgagca gccgctcgtc cacacgacgt cgccgtgctt
     4501 cgcgtcgcga tcgccgagcg cggccagcag ctttccgcgc gcggtgtcga tgtgcgagcg
     4561 cgctgcggcg ccttgcgggt gcgaggagct cgggttgccc caggcggtcc gcatcacttc
     4621 cgtcatgagg gcgagcacgt catcggagac gcgggtcgag gcggcattgt cgaggtagat
     4681 tggcggcatc tccgacttag gcgtctacct ccattatccg tggtgccgca agctgtgccc
     4741 gtactgcgat ttcgcggttg aggtcggtgc gcccgagcac gatgcgtatt tggctggcgt
     4801 cttgcgcgag ctggaggcga gggcgggtca gttttccggc gagctcgtgt cgatctatct
     4861 gggaggcggg acgccgtcaa tctgggatcc ggcgtgcgtc ggaacggcga tcgcggcgat
     4921 ccgcgcgaag ttcggcgagg cggcgctgcg cgaggtgacg ctcgaggcaa acccgacgga
     4981 ttgcaccagc gagaggctcg ccgcgtggcg cgacgccggt atcaacagac tgtcgatcgg
     5041 cgtgcagtcg ctcgcgcagg aagagctcgt cacgctcggt cgcgatcacc ggtttggcga
     5101 tggcatggcc gcgatcgagc gcgtgctcgc gttcggcggc ttcaccgtct cggcggactt
     5161 catcctcggc gtgcctccgc gccgcatgcc cgactgggcg gccgtgatcg cgctgccgat
     5221 cgatcatttg agcgtctacg agctgacgat cgaagatcgc accgcgtttg gtcagcgcgt
     5281 gaaaagcgga cgcctcgttc cgctcgacga ggaagcgctg atggagctct acgtcgcgac
     5341 gcaccgcacg ctcaccgccg ccggcttcga gcactacgag atctcgagct acgcgcggcc
     5401 gggcaagcgc gcggtgcaca actcgctcta ctggagcggc gccccgtttc tcgggctcgg
     5461 tgtcggcgcg gcgtcactcg cgctccatgc cgatggcagc ggcgagcgcg cgacgaaccc
     5521 gcgcaaggct cgcgactatc tggcggcgcc cggcgtcgcg gcggaggtca cggcgattcc
     5581 ggcggccgag atggcgaccg atcgcgcgtg gcttggcatg cggaccgtcg acggactcgt
     5641 cgaaccggcg ctgccggcgg aggtcgtgcg ctggctcctc gacgagaagc tggcggaacg
     5701 caggcagtta ccgggcaccg gtgtctaccg gatctgcccg actctgcgtg gctttctgat
     5761 ggcagatcgg atcgcggcgc ggatcgtcga ggcgttgtaa gctcgttgga ctcgaatggg
     5821 tggcaacgag ctgtctcggc gcgcgcagaa gatcctccaa gcggtggtga cggagtatct
     5881 gcagggcgga gatgcggtcg gatcgcgcac ggtgacgcgt cgccacgacc tcggcctctc
     5941 gcccgcgacg gtgcggaacg tgatggccga cctcgaggag atgggcctgc tcgagcagcg
     6001 tcacacgtcg gccggccgcg ttccgacgac gagcggcctg cgcctctacc tcgacagctt
     6061 gctcaaagtt cgcggcctca acccgcgcga gaaggacgag attcgcgagc ggatcaccgc
     6121 ggggacgccc gacgaggtca tgcagcgcgc gtcgcgaatg ctcgcggatc tcacgcatca
     6181 cgcggcggtg atcctcgcgc ccgatcccga ccagacgcgc ctcgagcgca tcgagttcat
     6241 cccgcttcgc gacggcaagc tgatcgccgt gctcgtcacg accgacgggc gcatcgagaa
     6301 ccgcctgctc ctcgaggacg tcgagccgag ccgcctcgag cgcattcaca actacctcaa
     6361 ccacctcctc tcgggcatga cgatcgacga ggttcgcgag cgcgtcatcc gcgagctcgg
     6421 cgaggacaag aaccagtatg acgaggccgt cgccacggcg ctgcgcctcg gccacgcgct
     6481 gttcgcgagc cagccgaagc aggacgtcgt cgtgacgggc cacgccaact tgatcgatgc
     6541 gagcgaggac tcgcagcccg atcgcatccg cgagctgttg cgtacgctcg aggacaaaga
     6601 ggtgctcgtc cgcctcctcg atcgcacgcg ccaggcggaa ggcctccagg tcttcctcgg
     6661 cgccgagacg gccatgcagg cgctgtcgag cagctccgtt gtcgcgatgc catatggccc
     6721 cgacgagcag ccgatcggtg cgctcgcggt gatcggcccg atgcgaatga actacggcaa
     6781 ggtgatgtcc gtcgtcgact acaccgccga tacggttagc cagctgctcg cggagctggg
     6841 ccgtggcagt aactcttagg ttgcggtaag ttccgccacg atgagcggag atgcttccca
     6901 ggacgacacc aagcccgcct ccgaggaggc cacgaagggc gacggcgacg ttctacccgt
     6961 cgaagtagag gcgcccgacg ccaagcccga ggaggcgaag gaagccgcga agcccgatcc
     7021 cgtggcagag ctcgaggcga agctcgcggc ggtagagaaa gagaagaagg acaactggga
     7081 gaagtacctt cgctcggccg ccgacatcga gaacatgcgc aagcggcaga agcgcgagct
     7141 cgaggacgcg aagaccgagt cgaagttcaa ggtcctgaag gagatgctcc cggtcgtcga
     7201 taacctcgag cgcgccgtcg agcacgcgac gcagggtggc gacgtcgacc ccaagctcaa
     7261 gggctttgtc gaaggcgtgc agctcgtgct tcgtcagttc agcaacgcat tcgagcgcct
     7321 cgaagtgcag tcggtcgacg cgatggatca accgttcgat ccaaaccagc acgaagcgat
     7381 ctcgcagcag gagagcgaca aggctccggg cacggtcgtc caggtgctgc agcgtggcta
     7441 caagtcgggt gaccgcttgc tccgtccggc actggtcgtc gtagcgaagg cgaaggcgca
     7501 gacggaatga gccgggtcgt cggcatcgac ctcggtacta ccaactcctg cgtcgcgatc
     7561 atggacggcg atcagcccgt cgtcatcgcg aatgcggagg gcacgcgcac gactccatcg
     7621 gtcgtcggct ttgcgccgtc gggcgagcgg ctcgtcgggc aagcagcgcg ccgtcaggcg
     7681 atgacgaacg ccgagaacac gatctacgcc gtgaagcgcc tgatggggcg caagtacgag
     7741 gactccgaag tccagcgtca cgtgctgacg tgcccgtacg aggttgtcgg cgcgccgaac
     7801 ggcgatgcac acgtgcgcgt gcgcggccgt gactactcgc cgccagaagt cagcgcgatc
     7861 atcctgcaga agatgcggca gaccgccgag gattggctcg gcgaagaagt caccgaggcc
     7921 gtcgtcacgg tgcccgccta tttcgacgat gcgcagcgcc aagcgacgaa agacgcaggc
     7981 cgcatcgcgg gcctcaacgt gcttcgcatc gtcaacgagc cgacggcggc cgcgctcgcg
     8041 tacggcgtcg agcaacaagg cgcgtcgaaa gtcgccgtct acgatctcgg cggcggcacg
     8101 ttcgatatct ccatcctcga gctcagtgac ggcgtgttcc gcgtgcgctc gacggcgggc
     8161 gacacgttcc tcggcggcga agacttcgac aacgcgatcg tcgagcacat gctcgccgcg
     8221 ttcatggaac aaaacgacgg catggacctg cgcggcgatc gcatggcgct gcagcgtctc
     8281 aaagatgcag ccgagcgcgc gaagatcgag ctgtcgagct cgatggtcac cgagatcaac
     8341 ctgccgttcc tcgctgcagg caccgacggt caaccgcgcc acctcatcat gcagatgctg
     8401 cgcgacgacc tcgagactct cgtcgagccg ctcgtgcagg cgacgctcga accgtgccgt
     8461 cgcgcgctcg aagatgcggg cctctctccg atcgagatcg acgccgtcat cctcgtcggt
     8521 ggccaaacgc gcatgccgcg cgtgcacacc gtcgtcgccg agatgttcgg caaagaggca
     8581 agccgctggg tgaaccccga cgaggtcgtc gcggtcggcg ccgcggtcca ggccggcgtg
     8641 ctcaccggcg aagtccaaga agtgcttctg ctcgacgtga cgccgctgtc gctcggtatc
     8701 gagacgatgg gtggagtctt cacgcgcctc atcgatcgca atacgtcgct gcccgcgcgc
     8761 gcgaccgaag tgttctcgac caccgtcgac aatcagccat tcgtcaacgt gcacgtgctg
     8821 caaggcgagc gcgagatggc ggccgacaac aagtcgctcg cgcagttcga gctgaccggc
     8881 atcccgcccg caccacgggg cgttcccaag atcgaagtcg cgttcgacat cgacgccgac
     8941 ggcgtgcttg ccgtatccgc gcgcgacatg gtcacgggcc gcagtcacgt cgtcaccgtg
     9001 cagccgacga ccggcctctc cgaccacgac atcgagcgcc tcgttgccga atccgagacg
     9061 atgcagaacg acgacgccgt tcgccgcctc atcgtcgaag cgcgcaacaa ggccgagggc
     9121 ctcttctatt cgagcgacaa agcgatggcg gagttcggcc agatgctcgc tccggaagaa
     9181 ttcgacttct tgacgacgga gcttgcggag tgccgcaccg ctctacagac cgaagacctg
     9241 ccaaccgtcg aagatgccgt tgcacgcctc gaggtcgctg cccaacgtat cggtgaagcc
     9301 atttacgcgg ctgccgacgg cgggggtagc gatatcggcg ggggcatgta agaaagccct
     9361 caatggcgaa gcgaaagcgc gactactacg aagtcctcgg tgtcgaccgt agcgccaccg
     9421 aaggcgacgt caagcgcgcg ttccgtgagc tcgctcgcca gcatcacccc gacgtgaatc
     9481 cgcccgacgc cggcgaacgc tttcgcgaga tcaacgaggc ctacgccgtc ctctccgata
     9541 aagatcagcg cgctcgctac gaccgctggg gccaccccga cgacggcggc tcgggcctcg
     9601 gcgcggtggt cgacgccgca caagagatga ttcgcgacgt cctccgccgc ggccgctcga
     9661 agccaaaagg ccgcgacctc cgctacacgc tcgaagtcac gttcgaagaa gccgcattcg
     9721 gcgcaacgaa gacgatccaa atcccaggtg ccgacggcaa gccgcgcgac ttctccgtcg
     9781 tcgtgcccgc gtcgacgaag gacggcacgg tcaaacaatt ccgcggcgaa ggcgaaccct
     9841 cgcgcgcagg cggcgccgcc ggcgacctcc acgtcatcgt gcgcatcgcc gagcacaccg
     9901 ccttccgccg cgacggcacc gacgtccaca gcgacttcac gatccagttc acgcaagccg
     9961 ccctcggcgc ggtcatcgat ctcccaacgc tcgacggccc cgtgaagatg cgcatccccg
    10021 aaggcacgca accgggtcgc gtcttccgga tccgcggtcg cggcatcccg caagcgccgg
    10081 gcaaaaatgc gccgcgcggc gaccacctcg tacacatcaa catcgaggtc ccgaccgagc
    10141 tgacgccgcg ccaaaagcag ctcatcgaag agctcgcgaa ggaatcgggc acctcggatg
    10201 tcgcgccggg caagcccggg cagaagacgc tgctcggtcg agtgcgctcg ctgctcgagg
    10261 agtagcgcgc tccgtgccgg gctccgctcc gtcggcttcg tcgacgaaca gtgtgtcggt
    10321 cgcctcgccg agtgcgtgcg ccccggaagc agggcaaggg ggcaaggaga gcaagggacg
    10381 cgagtcggag gatcacggcg ccttcggcgc gttcgctcgc gcggacaggg cgcgtgctgg
    10441 agagattgaa cacggagaca cgaagcacac ggagatcgag acagggtttg gcgccttccg
    10501 cgcgtttgct cgcacgggca gtccgcacgg gcagggcgtg tgctcgcaga tttatcgcag
    10561 agagggcgca gagagcgcag agagcgcaga ggatcagggc gtcttcggcg cattcgctcg
    10621 cacggacagg gcgcgtgctc gaaagattga acacggagac ccgaagcagt cggagatcaa
    10681 agctcggacg ccaaaggcat ttgggccgca cacatccgtg aaccggtcct tctttgtttc
    10741 ctgtctcggc tccgtgtgct ccgtgtctcc gtgtttgatt gttctgggct tctgcgtcgt
    10801 gatgacgggg tgcgggccca aggtgccgag gggcacgtgg gtcgagcacg aggagctggg
    10861 gcgcgcacat tcctcgacga atggcggcag tggggcgacg agcgccggca gcgcgaccgc
    10921 gaagcccatc gcgatcgacc tcgcgaagct cgacgccgcg acgatcgacc agctcgatta
    10981 tgcgacgtgt gtggcggtga tcgagcagct tggtgatcgc aagcccgcgg cacgcgtggc
    11041 gttgagggcg gcgcggttgg cgcatcatca gggcgatgat gtagatgcgc gtgcgttgat
    11101 cgcgcgcgcg acgaatgcgg cggatgaggg cgaggtgcat gcggagcttg cggcgttggc
    11161 ggcggaggcg gcggagacgc cggtcgatcg caaggtgatc gcggtgctgc tgccgctgag
    11221 tgggcggttt gcggcgttgg ggagcgagct gcgcgtggcg atcgagctcg cgccgcccga
    11281 gggagtgagc tggctgttcg tcgacacgaa gggcgagccc gatggagcgg tggcggccgt
    11341 cgagagcgcg tggaagaagg gcgcggttgg gatattgggg ccggtaggta cgcgcgaggc
    11401 gactgcggcg gcgagggcgg cggcgctgca tggaatgccg atcggattgc tggcgcctgc
    11461 ggatggtgcg gatgcggcga gcggcgtgtt tcggctcgtc ggttcgcccg cggatgaggg
    11521 cagggccgtg gcgaggatcg cgcaggccga tagcttcgcg accgtcgcgg tgttcgcgcc
    11581 gcgcgatgat gtcgggcagg agactgcaga tgcgtttgcg gcggaagcgg tgcggctcgg
    11641 gctgcgcgtg acgaagaccg cgaactacga tccgacggac gggaatttgg agcccgacgt
    11701 gaagcagctc ctagatctcg tgccggcgcg caatccgcgc ttggcggagc atctcgcgaa
    11761 gaatccgaag aacgggtgga agacgttctc gcccgatgtg ccgttctcgc tgctctacat
    11821 tccggaccgc tacgaccgcg cggcgattgt cgcggcattt ctgccgtact acggcgtcga
    11881 gctgcggacc gccgagaaca tgaattcggc gcagctgcag cgcaagcatg gcgggcatgt
    11941 gccgacggtg gtgcagctcg tcggtggtgc gggctggcat catccgtcgc tgcccgtgcg
    12001 cggcggcgag gcggtgcagg gcgcgctcat cgtcgacgcg tttgcgggtg gtgatggcgg
    12061 cgagatcgcg gcggacttcg tggccgcgtt tcagcagaag acgaatcgca ctccgagcgc
    12121 agcggcggca gaagcgttcg atgcggcgac gttgatggcg aaggcgcgtg cggcggtggg
    12181 catgacgtcg cccgatccgc gtggtgcgct gcggactgcg atcgcgaggg cgaagctcga
    12241 cgacggcgcg tgtggacctg cgagcatgga tggcgatggc gagctcgctc gcgagccggc
    12301 attgctcgag gtgcaggctg atcagatcgt gctcgtgcct tagccggcct ttgcgagcgt
    12361 cacgccgcgc gcgccctcga gaagct
//
LOCUS       DATLBZ010000431         7231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6975512, whole genome shotgun sequence.
ACCESSION   DATLBZ010000431 DATLBZ010000000
VERSION     DATLBZ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6975512"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1399)
                     /locus_tag="VMZ53_21870"
     CDS             complement(110..1399)
                     /locus_tag="VMZ53_21870"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HUS31180.1"
                     /translation="MRRLLPVLPVLLLAACEGKSSAPPAPVATTAPSPAPTQEKPVVT
                     SPSDPNAFVKGHNNFAVELYGKLPSGNVAASPASITIALAMTWAGAKGATADEIAKTL
                     HFVGDQPEVLSRWGHITEALAATKGPFVLRLANRLFVDKHRELDTVFFDINMKTFGAR
                     TDVFDFQAEPDDARQKINFWVAQQTEQRIKDLLPPRSITSATRLVVVNAIYMLAEWQT
                     PFDPNATRAKPFHVSATEAKQVPTMHRLGNYRYAKGDGAALLELPYKHLEFDVVPDVA
                     GELAMYVLLPDAVDGLPAIEAKLADTIRALQGKLAETRVSIALPKFTIDPPGPLELQQ
                     TLGELGIKTAFTPAADFSGITKTTTEPLFISNVMHKAFVKVDEKGTEAAAATAIGAPY
                     GAPPAPGTPFVADHPFVFMIIDRSSGLILFIGRVASP"
     gene            complement(1399..1611)
                     /locus_tag="VMZ53_21875"
     CDS             complement(1399..1611)
                     /locus_tag="VMZ53_21875"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HUS31181.1"
                     /translation="MSMSEDQYERLQDLEIKLAFQDKLIRELDALVRTFGDRLDKTNR
                     ELEALKQSIRSPETPLGPANEPPPHY"
     gene            1661..2260
                     /gene="tpx"
                     /locus_tag="VMZ53_21880"
     CDS             1661..2260
                     /gene="tpx"
                     /locus_tag="VMZ53_21880"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HUS31182.1"
                     /translation="MRNLALLVVVVAACGKSSGDTKPSAPARTERTDVIAREGTPLTL
                     LGPTLDVGDSMPDVAVYDAKLTPIQLGSLKGKIVVLSVVPSIDTRVCESQTHHMSNLM
                     TDMPPNVEVMTVSRDLPFAQQRFAEEAMTKTRFGSDYKEREFGRAFGVDVKETGLLAR
                     SVWVIGTDGKVAYRQLVANQASEPDYDALMAAVKRAAGA"
     gene            2281..3735
                     /locus_tag="VMZ53_21885"
     CDS             2281..3735
                     /locus_tag="VMZ53_21885"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HUS31183.1"
                     /translation="MSSDMVKIARRVLEENGFEPDAPTQIESLGNRDPGAGAEDMRDL
                     PWSSIDNEESRDLDQIEWAEQQKDGSIRLLLGIADVASFVHKGSPIDQHAAKNTTSLY
                     TGVKTFHMLPKALSTQRTSLLQDEERLAIVTEMFVPEDGNVDDKRTRIWSARVVNHAK
                     MVYERVGAWLEGDKSAAPSDPVMAKQLELQAEATRRLRARRIEAGALELETIEAQTVA
                     KNGKVIDLRLMHKNRARDLIEDIMIAANVATARYLESHGYSSIRRVVQAPSRWPRIVE
                     IARSLGTELPANPDVKALGDFVSAQRRSHPDTFADLSLTIVKLLGAGEYQLQRSKDPD
                     TGHFGLAVSDYMHSTAPNRRYADLVTQRILLASYAKRASEYSDDELAEIAARCTEREN
                     AANKVERTMRKVAAADLLHDRIGDTFDAIVTGNKPHATYARLLRPPAEGRIVRDESGL
                     DVGDKIKVRLLATDIEKGFIDFAAVDRHAAAAPQ"
     gene            complement(4294..5688)
                     /locus_tag="VMZ53_21890"
     CDS             complement(4294..5688)
                     /locus_tag="VMZ53_21890"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HUS31184.1"
                     /translation="MSRDALVKVERNLVAQHTQIDRALRRIAVAHAALDADEAHWLRI
                     AEEQRIWPKLGYVHGFEYLEDVFGYAPRTASERLRVARELGDLPQLEDALRCGVMSWS
                     VVRELTRVATPATVDHWIEEARGRRLRDVEQMVAGRKKGDEPGTPPDPKRVKHRVVLE
                     LDGESLVIWRQMHQVVEAEDDCNLDDRAFVLALGARILSVADCANDSRTVAANENDNE
                     NENENDNENDNENETPTSTTPTRKPMLRSVPARYMVHVTTCRDCGRGWQDGAGVRVEL
                     DRSAVDRAQCNAVICDDEKGQRPSRTIPARIERLVLERAQQRCEVPGCRSSKHLAIHH
                     IELWANGGTHDLWNLIVLCDGHHRVLHDGLLKITGRAPDQLVFERNGSPLAGHDTHAT
                     RKHVTGGAQVKKPMTAPNLKGEDPVALAQAALRQLGFKAQVAARAVDAACAHVGPNVE
                     LGALIKEALRQCGK"
     gene            complement(5952..6269)
                     /locus_tag="VMZ53_21895"
     CDS             complement(5952..6269)
                     /locus_tag="VMZ53_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31185.1"
                     /translation="MGAALFVASAHVGAALFVASAPIVHREVPTCATSWRPFFRRVAQ
                     SAWAGGEQRAPAFQTARGARCDLAAGPARLDRFARFPNLEVCGAPARAIADSLRSAGS
                     RHR"
BASE COUNT         1295 a         2442 c         2306 g         1188 t
ORIGIN      
        1 gatcaagggc agcacgctgc aaggcacgtg gggccacgcg cagtcgaaca gcgacggtgg
       61 tccttggacg ctcgagcgca aaaccaagcg ctcgcagatc gcgaactgat cagggcgacg
      121 cgacgcggcc gatgaacagg atcagcccgc tcgatcgatc gatgatcatg aagacgaacg
      181 ggtgatcggc gacgaacgga gtgccgggcg caggcggtgc accgtacgga gcgccgatcg
      241 cggtcgcagc cgcagcctcg gttcctttct cgtcgacctt cacgaatgcc ttgtgcatca
      301 cgttcgagat gaacagcggc tcggtcgtcg tcttcgtgat gccactgaag tcggcagccg
      361 gcgtgaacgc cgtcttgatt ccgagctcgc cgagcgtctg ctgcagctcg agcggtcccg
      421 gcggatcgat cgtgaacttc ggcagcgcga tcgagacgcg tgtctctgcg agcttgccct
      481 gcagcgcgcg gatcgtgtcg gcgagcttcg cttcgatcgc gggtagaccg tcgacggcgt
      541 cgggcaacag cacgtacatc gcgagctcgc ccgcgacatc gggcacgaca tcgaattcga
      601 gatgcttgta cggaagctcg agcagcgcgg cgccatctcc cttcgcgtag cggtagttgc
      661 cgagccgatg catcgtcggc acttgtttcg cctcggtcgc cgagacatga aacggcttgg
      721 ctcgcgtcgc gttcggatcg aacggcgtct gccattcggc gagcatgtag atcgcgttga
      781 cgacgacgag ccgcgtcgca ctcgtgatcg agcgcggtgg caacagatct ttgatgcgct
      841 gctcggtctg ctgtgcgacc cagaagttga tcttctggcg cgcgtcgtcg ggctcggctt
      901 ggaagtcgaa gacatcggtc cgcgcgccga atgtcttcat gttgatgtcg aagaagacgg
      961 tgtcgagctc gcggtgcttg tcgacgaaca gccgattcgc gagccgcagc acgaacggcc
     1021 ccttcgtcgc cgcgagcgct tccgtgatgt ggccccagcg cgagaggact tcgggctgat
     1081 cgccgacgaa gtggagcgtc ttggcgattt cgtctgcggt cgcgcccttc gcgccggccc
     1141 acgtcatcgc gagcgcgatc gtgatgctgg cgggcgatgc tgcgacgttg ccactcggga
     1201 gcttgccgta gagctcgacc gcaaaattgt tatggccttt gacgaacgcg ttcggatccg
     1261 acggactcgt gaccacaggt ttctcctgag taggcgcggg agatggagcg gtcgtcgcga
     1321 cgggcgcagg tggcgccgac gacttgcctt cgcatgcagc gaggaggagg acgggcagga
     1381 cgggcaggag gcgacgcatc agtaatgagg aggcggctcg ttggccgggc cgagcggagt
     1441 ttcgggagag cgaatcgatt gcttgagcgc ttcgagctcg cggtttgtct tatcgaggcg
     1501 gtcgccgaac gtgcggacga gcgcgtcgag ctcgcggatc agcttgtcct ggaacgcgag
     1561 cttgatctcg aggtcttgca ggcgctcgta ctggtcctcg ctcatgctca tgcggggacg
     1621 gtatcagagc gctcggcccg acacctgcta cgatccagcc gtgcggaatc tagcccttct
     1681 cgtcgtcgtc gtcgccgcgt gtggcaagtc ctcgggtgac accaagccga gcgcacccgc
     1741 gcgcacggaa cgcacggacg tcatcgcccg cgagggaaca ccgctgacgc tgctcggccc
     1801 cacgctcgat gtcggtgact cgatgcccga tgtcgcggtc tacgacgcga agctcacgcc
     1861 gatccagctc ggctcgttga agggcaagat cgtcgtgctg tccgtcgtgc cgtcgatcga
     1921 cacgcgcgtc tgcgagagcc aaacccacca catgagcaac ctcatgacgg acatgccgcc
     1981 gaacgtcgag gtgatgaccg tcagccgcga cctgccattc gcgcaacagc ggttcgcgga
     2041 agaggcgatg acgaagacga ggttcggctc cgactacaag gagcgggagt tcggtcgggc
     2101 atttggcgtg gacgtcaagg agacgggcct gctcgcccgc agtgtttggg tcatcggcac
     2161 cgacggcaag gttgcatatc gccagctcgt cgcgaaccag gcgagcgagc ctgactacga
     2221 cgcgctgatg gccgccgtga agcgcgcagc cggcgcctga atcagtggat actgccggcc
     2281 atgtcgtccg acatggtcaa aatcgcgcgc cgcgtgctcg aggaaaacgg tttcgagccc
     2341 gacgctccga cccagatcga gtcactcgga aatcgcgacc cgggcgccgg cgccgaagac
     2401 atgcgcgatc tgccgtggtc gtcgatcgat aacgaagagt cgcgcgacct cgatcagatc
     2461 gagtgggccg aacaacaaaa ggacggctcg atccgccttc tcctcggcat cgccgatgtc
     2521 gcgtcgttcg tccataaagg ctcgccgatc gatcagcacg cggcgaagaa cacgacctct
     2581 ctgtacacgg gcgtgaagac gttccacatg ctgccgaagg cactctccac gcagcgcacc
     2641 tcgctgctgc aagacgaaga gcgcctggcg atcgtgaccg agatgttcgt ccccgaagac
     2701 ggcaacgtcg acgacaagcg cacgcgcatc tggtccgctc gcgtggtcaa tcacgcgaag
     2761 atggtctacg agcgcgtggg cgcgtggctc gaaggcgaca agtccgccgc accaagcgat
     2821 ccggtgatgg cgaagcagct cgagctgcaa gccgaagcca cccgccgcct ccgcgctcgc
     2881 cgcatcgaag cgggcgctct cgagctcgag acgatcgagg cgcagaccgt cgcaaagaac
     2941 ggcaaggtca tcgacctgcg cctgatgcac aagaatcgcg cgcgcgacct catcgaggac
     3001 atcatgatcg cggcgaacgt cgcgaccgcg cgctacctag aaagccacgg ctactcgtcg
     3061 attcgccgcg tcgtgcaagc accgtcgcgg tggccgcgca tcgtcgagat cgcgcgctcg
     3121 ctcggcaccg agctgcccgc caaccccgac gtcaaagccc tcggcgactt cgtctccgcg
     3181 caacgccgct cgcacccaga cacattcgcc gacctctcgc tcacgatcgt caagctcctc
     3241 ggcgcgggcg agtaccagct ccaacgctcg aaggatccag acaccggcca cttcggcctc
     3301 gctgtcagcg actacatgca ctcgacggcg ccgaaccgcc gctacgccga cctcgtcacg
     3361 cagcgcatcc tgctggcctc atacgccaag cgcgcgagcg aatacagcga cgacgagctc
     3421 gccgaaatcg ccgcgcgctg cacggagcgc gagaacgcag cgaacaaggt cgagcgcacg
     3481 atgcgcaaag ttgcggccgc cgacctcctc cacgaccgca tcggcgacac gttcgacgcg
     3541 atcgtcacgg gcaacaagcc gcacgcgacc tacgcgcgcc tcctccgccc acccgccgag
     3601 ggccgcatcg ttcgcgacga gtccggcctc gacgtcggcg acaagatcaa ggtccgcctc
     3661 ctcgcgacgg acatcgagaa aggcttcatc gacttcgccg ctgtcgatcg ccacgccgct
     3721 gcggcgccgc aatagcgccg ctccgccccg ccccgccgct gcgctccgcc cctgctccgc
     3781 ggttgcgctc ggccgcgccg ccactgcgct cttcgaagac cggatccgga tctaacgcaa
     3841 aggcgcaaag agggcagagc acgcagaaac aggttagggg cgcgcacggg catcgcgcac
     3901 gggcatcgcg cacgggcatc gcgcggaccc ggatggaaca caaaggcgca gagaaggcag
     3961 aggacgcaga acaggttagg ggcgcacgcg ggcaagacgt gtgcttcaga tacgtcgaca
     4021 cccggcttat cgatcccgcg atccccgcga tcccgcgcac cctgcgatcc ctgcgctcct
     4081 gcgagccctg ctctgcgttg gatctccgag cacccggagc gcccgtgcgg gtgaacgcgc
     4141 cgaaggcgcg cccttttctc ctagcgccct ctgccctcat tgcgcctttg tgttccatcc
     4201 ggggccacgt gttccatccg gggccacgtg ctcccgtccg tgctgcccgt gcgcgatgtt
     4261 cgcacgcggt gccgtgcgtg cgtacgcagc tcatcatttg ccgcactgcc gcagcgcctc
     4321 cttgatcagc gcgccgagct cgacgttcgg gcccacgtgg gcacacgccg catcgaccgc
     4381 gcgcgccgcg acttgcgcct tgaagccgag ctgccgaagg gcggcttgcg cgagcgcgac
     4441 cggatcctct cctttgaggt tgggcgcggt catcggcttc ttcacctgcg cgccccccgt
     4501 tacgtgcttt cgcgtcgcgt gcgtatcgtg acccgcaagc ggcgaaccgt tgcgttcgaa
     4561 tacgagctgg tcaggtgctc tccccgtaat cttcaataga ccgtcgtgca acacgcgatg
     4621 gtgcccatcg cagagcacga tcagattcca gagatcgtgc gtcccgccgt tcgcccagag
     4681 ctcgatgtga tgaatcgcca ggtgcttcga cgacctgcat cccggcactt cgcatcgttg
     4741 ctgcgcacgc tcgagcacga gacgttcgat gcgcgcagga atcgtgcgcg acggccgctg
     4801 tcccttttcg tcgtcgcaga tgacggcgtt gcactgcgca cgatcgaccg ccgagcgatc
     4861 aagttcgacg cgcacgcccg cgccatcctg ccaaccgcgc ccacagtcgc gacacgtcgt
     4921 gacatggacc atgtaacggg ccggaacgct gcgaagcatc ggcttgcgag tcggcgtcgt
     4981 tgaagtgggg gtttcgttct cgttgtcgtt ctcgttgtcg ttctcgttct cgttctcgtt
     5041 gtcgttctcg ttcgccgcga ccgtccgcga gtcgttcgcg caatccgcaa cgctcaagat
     5101 gcgcgcgccg agcgccaaca cgaatgctcg atcgtcgagg ttgcagtcgt cctccgcctc
     5161 gacgacttga tgcatctgcc gccagatcac caagctctcc ccatcgagct cgagcaccac
     5221 acgatgcttc acgcgctttg gatcaggcgg cgtgcccggc tcatcgccct tcttgcgtcc
     5281 cgcaaccatc tgctcgacat cgcgcaacct ccgcccgcgc gcctcttcga tccagtgatc
     5341 gacggtcgct ggtgtcgcca cgcgcgtgag ctcgcggacc actgaccagc tcatcacgcc
     5401 gcatcgcaac gcatcctcca gctgcggtag atcaccgagc tcgcgtgcaa cacgaagacg
     5461 ctccgacgcg gttcgcggcg catatccgaa gacgtcctcc agatactcga agccgtgaac
     5521 gtagccgagc ttcggccaaa tgcgctgttc ctccgcaatt cgcagccagt gcgcttcgtc
     5581 ggcgtcgagg gctgcatgcg caaccgcgat tcgccgaagc gcgcgatcga tctgcgtgtg
     5641 ctgcgcaacg aggtttcgct ccaccttcac caatgcatcg cgtgacatga acatccacta
     5701 ccacgcgaaa aatcggcttt gccgttcgcg cggagcgcgc gaatttcgag attcctcgac
     5761 gaggaacgcc tcgcggtgat tgcgtgcgtt gcgtcgcgag gagcgcacgc acgcgtgcga
     5821 tcaattcgag tttgtcgtcg cgtaaacgag tacgccgcga aacgcgtcaa caagaaagtt
     5881 gtcaaacatt gtcgtcgaga gagcgctcgc gtggcggcac tcgatccgtc ggtgcacacg
     5941 cgcgatcgat gtcagcgatg gcgcgagccg gcgctccgca aactgtcagc gatggcgcga
     6001 gccggcgctc cgcaaacttc gagattcggg aaacgagcaa aacggtcgag ccgcgccggg
     6061 ccggcggcga ggtcgcagcg cgcgcccctg gccgtttgga aggccggggc gcgctgctcc
     6121 ccgccggccc aggcgctctg agcaacacgg cggaagaaag gacgccacga cgtcgcgcac
     6181 gtcggcactt cgcgatgaac aatcggggca ctcgcgacga acaacgccgc gcccacgtgc
     6241 gcactcgcga cgaacaacgc cgcgcccacg tgggcacatc atcacgatga accgtcgtgc
     6301 gtcgcgggca catcgtcacg acgaacaacg ctgcgcccac gcgggcactc gcgacgaaca
     6361 acgccgcgcc cacgtgggca tatcacgggc atgcggtcac gacgtgtccc gctcgcgctc
     6421 cgccgctgcg ctgcgctccg ccgctgctcc gccgctgcgc tcggccgccc cgccactgcg
     6481 ctcttcaaaa agcagatccg gatctaacgc aaaggcgcca agagggcaga gcacgcagaa
     6541 acaggttagg ggcgcgcacg ggcatcgcgc acgggcatcg cgcacgggca gcgcgcacgg
     6601 ggagcacgga cccggatgga gcgcaaaggc gcaaagaggg cagagcacgc agaaacaggt
     6661 tagggggcgc gcacgggcaa gacgtgtgct tcagatacgt cgacacccgg ctcatcgatc
     6721 cccgcgatcc ccgcgacccc tgcgatccct gcgatcctgc gatccctgct ctgctttgga
     6781 tctccgagca cccgcagcgc ccgtgcgggt gaacgcgccg aaggcgcgcc cttttctcct
     6841 tgcgacctct gccctcattg cgcctttgtg ttccatctgg gtctgggtct gcgtttgggt
     6901 ctgggtctgg gtctgggtct gggtctgggt ctgggtctgg ctctgggtct gggtctgcgt
     6961 ctggatccga tctaacgcaa aggcgtaaag agggcagagc acgcagaaac aggttagggg
     7021 gcgcgcacgg gcatcgcgca cgggcatcgc gcacgggcat cgcgcacggg catcgcgcac
     7081 gggcatcgcg cggacccgga tggaacacaa aggcggagag aaggcagagg acgcagaaac
     7141 aggttagggg cgcacgcggg caagacgtgt gcttcagata cgtcgacacc cggctcatcg
     7201 atccccgcga tcccgcgcac cctgcgatcc c
//
LOCUS       DATLBZ010000432        10344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4234636, whole genome shotgun sequence.
ACCESSION   DATLBZ010000432 DATLBZ010000000
VERSION     DATLBZ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4234636"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VMZ53_21900"
     CDS             complement(<1..888)
                     /locus_tag="VMZ53_21900"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31186.1"
                     /translation="MRDLVDELLHAKVKANLFGGDHAPTLGRLVIRERIGAGAMGTVF
                     EAFDPRLERKVAVKVLRADADAARVLMEARALAKLQHPNVVAIYDADELDGLVYIVME
                     HVTGASLRSWLDVTPNAANVANVANVANPPNAATAVNVRNWRDVVRVMRDAAAGIAAA
                     HAAGLVHRDIKPDNLLVGAEDSQTDTGRARGSNTERVHSAADRARSAADRARSAADRA
                     RSAAGRARVVDFGLAHVQLADDGTSAGTPFYMAPEVLAGAPATPASDQFSFGVTLFEL
                     LYGKRPHGALPTAHAATEPA"
     gene            915..1511
                     /locus_tag="VMZ53_21905"
     CDS             915..1511
                     /locus_tag="VMZ53_21905"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS31187.1"
                     /translation="MSDAADTVTDVALLDAWRSGDATAGERLVARHWTSVSRFFRSKI
                     GDDGADLISQTFLACVEARDRIEGGNVKAYLFAVARRRLADHFRKRARSPAIDVGASS
                     VIDLGTGPVTHVSRRQRHELLRDGLARIPLDDQIALELAYFEGLSTKDIAAVLEINEN
                     TVRSRLSRAREKLREALATLANSDDAAFAESQLDEKPR"
     gene            complement(1557..2993)
                     /locus_tag="VMZ53_21910"
     CDS             complement(1557..2993)
                     /locus_tag="VMZ53_21910"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HUS31188.1"
                     /translation="MHLSDLDATAQAELVKKGDASPRELVDAAIARIEKHNPALGAVI
                     IPLFEQARATADSAPHGPFRGVPILIKDINSTIGGVLYTAGLDPLRRANYKSPVDSHL
                     VALLKRAGFVIVGKTNTSELGILPSAEPPAWPPTRNPYDPSRSTGGSSGGSACAVATG
                     MVPVAHANDGGGSIRIPASCCGLFGLKPSRGRVSFAPHYGEVNGGLVNEHVVARSVRD
                     SAAILDILAGTVPGDPYTAPDQLRPYLSELTVPPAKLKIGFATKHITPESPALVDSHP
                     DCVAAVQHTAKLLESLGHHVEQADITATHDPEWVPNFLSIWAVGVTQKLDWASRVLGR
                     PIEMHEIEPLTYGLAELGRLIGGAAYADAWDWLHGATRRIATFFERYDLWLTPTVTEP
                     PVPLGTFRSPPDDILAGIFRAADFAPFTAIFNSTGQPACSLPLYRNAAGLPIGVQLAA
                     AYGREDLLFRVAAQLEAAQPFVHAATRH"
     gene            3033..3788
                     /locus_tag="VMZ53_21915"
     CDS             3033..3788
                     /locus_tag="VMZ53_21915"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS31189.1"
                     /translation="MQQLAGKQALVTGGGRGIGAACARGLAAAGAHVTVCGRTQADLD
                     AVAKEVGGRAIVCDVSDRAAMDRMVAEMQGRIDILVNNAGIAESASIEKTSDELWDRI
                     LEIDATAPFRLVRALVPKMCEAKWGRIINIASNAGVSGYGYSSAYCAAKHAMVGFTRA
                     LAIDLAKTGVTINALCPGWVHTQMSDEAVARIAAKTGRGIDDARKALESMSPQRRMIE
                     PEEVAHACVMLCAEGARGIHGQTIVIDGGAILK"
     gene            3785..4186
                     /locus_tag="VMZ53_21920"
     CDS             3785..4186
                     /locus_tag="VMZ53_21920"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUS31190.1"
                     /translation="MSADDVVVPGPPPKGYSNGRIGRGAVLHVAGQVGWNEQLKFEHK
                     DLVPQFAKALDNVLAVVAAAQGKPTDIAEMTVYVTDLDAYRRGRQELGAIWKQRLGRH
                     YPAMALVEVKGLLEPDALVEIQAVAYVESAP"
     gene            4183..5016
                     /locus_tag="VMZ53_21925"
     CDS             4183..5016
                     /locus_tag="VMZ53_21925"
                     /inference="COORDINATES: protein motif:HMM:NF006107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HUS31191.1"
                     /translation="MTGAAQMIQPQTFAMELAKGVATITLDRPKRLNALTFEVYRELA
                     ETFEKLDSYPEVRAVVLTGRGKAFCSGGDQDDIIKELLGRDTPQLVSFTRATGRLIQA
                     MRECRRPIIGAINGTCVGAGAVMACACDMRILAKEARIGFIFPKVGLCGADMGATYLL
                     PRIVGLGHATELMFFGEMIDSAHAERIGFANRVVENGEEAVRVATEWAHKLAAGPAFA
                     HSMTKQMIESEHTMPLAAAIEAEAQAQALCMQHPDFAEAHAAFKDKRPPKFQGAPTER
                     E"
     gene            5020..6213
                     /locus_tag="VMZ53_21930"
     CDS             5020..6213
                     /locus_tag="VMZ53_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS31192.1"
                     /translation="MRLSSDDLPMFFDDAHRVLAERLRAAAPAIGEAENNADEAARDR
                     GAVDALARASLFELVAPASGPEWRAPDSLRGSGPTDSRSLCLAREMLGYVSPRADSIF
                     AVQGLGVHSILLAGTDEQRAHVTAYARGAGIAAFALTEPEAGSDVASIQTRAAPCAEG
                     YRLDGDKLFISNLGIADHATVVAATEPSLGAGGLTAFWLPLDTPGVTVKPLVATAPHP
                     IGALELRGVVLPASSKIGEVGQGMKLAMLTLDAFRVSVGAAAVGMARRAMDEAITFVT
                     RRMQFGKLLSEQPLIQAHIADMAVDLDAARLLVLRAAHRKDTTTDRVTTEVSIAKLGA
                     TEAAQRVIDRAVQLLGGRGVMAGSVVEHLYRAIRPLRIYEGTSEIQRTIIGRALTKPG
                     GARPS"
     gene            6210..7868
                     /locus_tag="VMZ53_21935"
     CDS             6210..7868
                     /locus_tag="VMZ53_21935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HUS31193.1"
                     /translation="MTVTFPEDFNLADYWLFAREREKKGSKIALRFGDRSWTYAEVAE
                     RSRGLARALIEAGLRPGERVYIVLPDTPAFVWSIFGTLAAGGVVAMGNPIAPVEDLAY
                     VVDYVRAAVVITTPTVAAALAPHIKDVHTLHALLLAPDAATGDDPEAPCEPIVSVPMC
                     EDLHKELRKGLASDIPLPKTHRDDQAIWLFTSGSTGRPKAAMHSHRDFAFNTEVFAKQ
                     TIAMHEDDVSVSVPRLFFGYATGTNLWFPFAVGATVGLFSERPTAESITAAIQRYKAT
                     VVTNVPTMLGKILDLDDELRAKGQRGLDLSSVRFHFSAGEALPEPLMRRFIERFGGQI
                     YDGIGSAEMFHIYCSNRPGDVKPGSLGRVVDGYELRILPEEANEPGAPELPRGEIGVL
                     WVKGDSVSHGYFRDRDKSWKTFHGHWCRTGDLFSIDADGYLYFQGRADDLFKVGGIFV
                     APREVEDCLLAHPAVSVCAVIPAEDGGLTKPKALVVLRPDARDKDKRALADELKAHVQ
                     ARLSKHKYPRWVLFVDDLPKNDRGKVDKKTLIEREKRGELSSDS"
     gene            7865..8626
                     /locus_tag="VMZ53_21940"
     CDS             7865..8626
                     /locus_tag="VMZ53_21940"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HUS31194.1"
                     /translation="MTEKFLARHTTATLEQLVTSKKGVIALVPVGSTEPHGPHLGLGT
                     DVIISAAACLRACELLEKRGTMIGVIAPAISYGVTECATGFKGAVSIPAAVLTQYIAA
                     VVDGLLASGVRHVCLVNNHLEPAHDAAVRAVLEGREKKVSYACPLTKKWARTLSAEFK
                     SGECHAGRYETSIVMAAAPELVQEPLRVRLSPVPVSLSKKLAEGINTFEAMGMELAYA
                     GDPASASVEEGEQLIQRLGDMVVGEVLESLGIKPA"
     gene            8636..8950
                     /locus_tag="VMZ53_21945"
     CDS             8636..8950
                     /locus_tag="VMZ53_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31195.1"
                     /translation="MHLTNSTLTIAVALVSSLYLFTQSTNRTFPLIATIVAGVELLLT
                     LGIMSLSLAKYRVDVILPALLAISGFVCWTKTTSKGGVTAASAVALIGAMQLLLALRL
                     FS"
     gene            8960..9481
                     /locus_tag="VMZ53_21950"
     CDS             8960..9481
                     /locus_tag="VMZ53_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31196.1"
                     /translation="MRLLCAALLLTAAACTDDSDPDVRVERQVATLDDASAAAWSQGG
                     AVLVGVPGTFAFPQPLSLRISIVDNKQLDEEMSPTQLKITSATLATYGDASELTVKTQ
                     PSCAANFCSAELEVSAQGASMLTIEADGPDGKQTECFYYAIYEADDPATAGATYRTEL
                     ETKQRECRATYWN"
     gene            9497..>10344
                     /locus_tag="VMZ53_21955"
     CDS             9497..>10344
                     /locus_tag="VMZ53_21955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31197.1"
                     /translation="MDRDPLTAIAWQFRTNQFGDLVKHVRDHTAMVGMELLALSGAKR
                     SATLPTVWSQLPRHPNVLEPLRGDQSRFLLRYAALNWTEPPFLRVDQERLAATQLFQL
                     ASVYQMLFETTGKNVGLFRNPLVKIDLGGFIRVCFLPMKPITVRPRVTVESDVDQQHL
                     VRLVGECARRSLSITEGSLRWDILERCLTHNKQHQYKSLEDLMAACRLAGIEYPVERT
                     GQLSAAWLEMEQGLGWLQLPQIRDYLDRASDHFGASLELVRLDYRDAQHELVRAVASW
                     GRGTCAV"
BASE COUNT         1763 a         3398 c         3451 g         1732 t
ORIGIN      
        1 cgcgggctcg gtcgcggcgt gtgccgtcgg cagagcacca tgcggccgct tgccgtacag
       61 aagctcgaac agcgtcacgc cgaagctgaa ctgatccgac gcgggcgttg caggtgcacc
      121 ggcgagcact tcgggcgcca tgtagaacgg cgtgcccgca ctcgtgccgt cgtcggcaag
      181 ctgcacgtgc gccaggccga aatcgacgac gcgcgcacgg cctgctgcgc tgcgggcgcg
      241 gtctgctgcg ctgcgggcgc ggtctgctgc gctgcgggcg cggtctgctg cgctgtgtac
      301 acgctccgtg ttcgagccgc gcgcgcgacc cgtgtcggtc tgcgagtcct cggcgcctac
      361 gaggaggttg tcgggcttga tgtcgcggtg gacgaggccc gccgcgtgag cagccgcgat
      421 gcccgcggca gcgtcgcgca tcacgcgtac gacatcgcgc cagttacgta cgttcaccgc
      481 cgtcgccgcg ttcggcgggt tcgcgacgtt cgcgacgttc gcgacgttcg cggcgttggg
      541 cgtgacgtcg agccacgagc gcagcgacgc gcccgtcacg tgctccatca cgatgtagac
      601 aaggccgtcg agctcgtcgg cgtcgtagat tgcgacgacg tttggatgct gcagctttgc
      661 gagcgcgcgg gcttccatga gaacacgcgc ggcgtcggcg tcggcgcgca gcaccttgac
      721 ggcgaccttg cgctcgagcc gcgggtcgaa tgcttcgaag accgtgccca tcgcacccgc
      781 gccgatgcgc tcgcggatga cgaggcggcc gagcgttggc gcgtggtcgc ctccgaagag
      841 attcgctttg accttcgcgt gcagcagctc gtcgaccaag tcgcgcaaca gctgcatggt
      901 ataccggccg cgtcgtgtct gacgccgccg acactgtcac cgacgtcgcg ctcctcgacg
      961 catggcgcag cggtgatgcg acggcgggcg agcggctggt ggcgcggcac tggactagcg
     1021 tgtcgcggtt ttttcgctcg aagattggcg acgacggcgc ggacctgatt tcgcagacgt
     1081 tcttggcgtg cgtcgaggcg cgtgatcgga tcgagggcgg gaatgtgaag gcgtacttgt
     1141 tcgccgtcgc gcgccggcgg ctcgcggatc actttcgcaa gcgtgcgcga agtccggcga
     1201 tcgatgttgg ggcgtcgtcg gtgatcgatt tggggactgg gccggtcacg catgtgtcga
     1261 ggcggcagcg tcacgagctc ttgcgcgacg gcttggcgcg cattccgctc gacgaccaga
     1321 tcgcgctcga gctcgcgtac ttcgagggac tgtcgacgaa ggacattgcc gcggtcctcg
     1381 agatcaacga gaacacggtg cgcagccggc tgtcgcgcgc gagagagaag ctgcgcgagg
     1441 cgctcgcgac gctcgcgaat tcggacgacg cagcgtttgc cgaatcgcag ctcgacgaga
     1501 aaccgcgctg aggtgtggcg gcagtggcgc gtggcggcgg cgggcgcgtc gcggcgtcag
     1561 tggcgcgtgg cggcgtgcac gaacggctgc gcggcctcga gctgcgcggc gacccggaat
     1621 agcaggtctt cgcggccgta cgcagcggcg agctgcacac cgatcggcag accggcggca
     1681 ttgcggtaga gcggcagcga gcacgcaggc tggcccgtcg agttgaagat cgccgtgaac
     1741 ggcgcgaagt ctgcggcgcg gaagatgccg gcgaggatgt cgtcgggcgg cgagcggaat
     1801 gtgccgagcg ggaccggcgg ctcggtgacc gtcggcgtga gccatagatc gtagcgctcg
     1861 aagaatgtcg cgatgcggcg cgtcgcaccg tggagccagt cccacgcatc ggcgtaggcc
     1921 gcgccgccga tcaggcgacc cagctcggcg aggccgtatg tgaggggctc gatctcgtgc
     1981 atctcgatcg gacggccgag cacgcgcgac gcccagtcga gcttctgcgt gacgccgact
     2041 gcccagatcg agaggaagtt cggcacccat tctggatcgt gcgtcgcagt aatgtcggct
     2101 tgctcgacgt ggtggccgag cgactcgagc agctttgccg tgtgctgcac ggcggcgacg
     2161 caatcgggat gcgaatcgac gagcgcgggg ctctcgggcg tgatgtgctt cgtcgcgaat
     2221 ccgattttca gctttgccgg cgggaccgtt agctccgaga gatacgggcg gagctgatcg
     2281 ggcgccgtgt atggatcgcc tgggaccgtg cccgcgagaa tgtcgaggat cgcggcggag
     2341 tcgcgaaccg agcgcgcgac gacgtgctcg ttgacgagcc caccgttgac ctcgccgtaa
     2401 tgcggcgcga acgacacgcg ccctcggctc ggcttgagac cgaacaggcc gcagcaactc
     2461 gcggggatgc ggatcgatcc gccgccatcg ttcgcgtgcg cgacggggac catgcccgtt
     2521 gcaacggcgc acgccgagcc gccgctcgag ccacccgtcg agcgcgacgg atcgtacggg
     2581 tttcgcgtcg gaggccacgc cggtggttcg gcggatggca ggatgccgag ctcgctcgtg
     2641 ttcgtcttgc cgacgatgac gaagccggcg cgtttgagca gcgcgacgag gtggctgtcg
     2701 acgggcgact tgtagtttgc gcggcgaagc ggatcgaggc cggcggtata gaggacaccg
     2761 ccgatcgtcg agttgatgtc tttgatgagg atcggtacgc cgcggaacgg gccgtgaggt
     2821 gcggagtcag ccgtcgcgcg cgcctgctcg aacagcggga tgatgaccgc gccgagcgca
     2881 gggttgtgct tctcgatgcg tgcgatcgcg gcgtcgacga gctcgcgggg tgatgcatcc
     2941 cctttcttga cgagctcggc ttgcgcggtt gcgtcgaggt cggacaggtg catgagctgc
     3001 cggcgcagtg tcgcacgtgg tatgtccggc gcatgcaaca gctggccgga aagcaagcgc
     3061 tggtcaccgg tggtggccgc gggatcggcg ctgcgtgcgc acgtggtctc gcggcggccg
     3121 gcgcgcatgt gacggtgtgc ggccgcacgc aagcagacct cgatgcggtc gcgaaggaag
     3181 tcggtggtcg cgcgatcgtg tgcgacgtct cggatcgcgc tgcgatggat cgcatggtcg
     3241 ccgagatgca ggggcgcatc gacattctcg tcaacaacgc cggcatcgcc gagagcgcgt
     3301 cgatcgagaa gacgagcgac gagctgtggg atcgcatcct cgagatcgat gcgacggcgc
     3361 cgtttcggct cgtccgcgcg ctcgtgccga agatgtgcga agcgaagtgg ggacgcatta
     3421 tcaacatcgc gtcgaacgcc ggcgtgtccg gatacggcta ctcgtcggcg tactgcgctg
     3481 cgaagcacgc gatggttggc ttcacgcgcg cgctcgcgat cgatttggcg aagaccggcg
     3541 tcacgatcaa tgcgctgtgc ccgggctggg tccacacgca gatgtccgac gaagccgtgg
     3601 cgcgcatcgc cgcgaagaca ggccgcggca tcgacgacgc tcgcaaggct ctcgaatcga
     3661 tgagcccgca gcgccgcatg atcgagcccg aagaagtcgc gcacgcctgc gtgatgctgt
     3721 gcgccgaagg tgcgcgcggc attcacggcc agacgatcgt catcgacgga ggagcgattc
     3781 tcaaatgagc gccgacgacg ttgtggtgcc gggcccgcct ccgaagggct actcgaacgg
     3841 acgcatcggg cgcggcgctg tgttgcacgt cgccggtcag gtcggctgga atgaacagct
     3901 gaagttcgag cacaaggacc tcgttcctca gttcgcgaag gcgctcgata acgtgctcgc
     3961 cgtcgtcgcg gccgcgcagg gcaagccgac cgacatcgcg gagatgaccg tctacgtgac
     4021 cgacctcgac gcataccgcc gcggccgcca agagctcggc gcgatctgga aacagcgcct
     4081 cggacgtcac tatccggcga tggcgctcgt cgaggtgaag ggcttgctcg aaccggacgc
     4141 gctcgtcgag atccaggccg tcgcttacgt ggagtcagcg ccgtgacagg agcagcccag
     4201 atgatccagc cgcaaacatt tgccatggag ctcgcgaagg gcgtcgcgac gatcacgctc
     4261 gatcgaccga agcgtctcaa cgcgctgacg ttcgaggtgt atcgcgagct cgccgagacc
     4321 ttcgagaagc tcgactcgta tccagaggtg cgagcagtcg tgctcacggg gcgcggtaaa
     4381 gcgttctgct ccggcggcga tcaagacgac atcatcaagg agctgctcgg ccgcgatacg
     4441 ccgcagctcg tgagcttcac gcgcgcgacg gggcgcttga tccaggcgat gcgcgagtgt
     4501 cgtcgtccga tcatcggcgc gatcaatggc acgtgcgtcg gtgcgggcgc ggtcatggcg
     4561 tgcgcgtgcg acatgcgcat cctcgcgaag gaagcgcgca tcggcttcat cttcccgaag
     4621 gtcgggctct gcggcgccga catgggcgcg acgtacctac tgccgcgcat cgtgggtctc
     4681 ggccacgcga cggagctgat gttcttcggc gagatgatcg attcggcgca cgcggaacgc
     4741 atcggttttg ccaatcgcgt cgtcgagaac ggtgaggaag cggtgagggt tgcgaccgag
     4801 tgggctcaca agcttgcggc gggtccggcc ttcgcgcatt cgatgactaa gcagatgatc
     4861 gagagcgagc acacgatgcc gctggccgcc gcgatcgagg ccgaggccca ggcgcaggct
     4921 ctctgcatgc agcacccgga cttcgccgag gcgcatgccg cattcaagga caagcgaccg
     4981 ccgaagtttc agggcgcgcc gacggaacgc gagtgagcca tgcgtctgtc gagcgacgac
     5041 ctgccgatgt tcttcgacga tgcgcaccgc gtgctcgcgg aacgtttgcg tgctgcggct
     5101 cccgcgatcg gtgaagccga gaacaacgcc gacgaggccg cgcgcgatcg cggtgcggtc
     5161 gatgcgctcg cacgagcgag cctgttcgag ctggtggcgc cggcgtctgg cccagaatgg
     5221 agagccccgg attcactccg gggctcgggg ccgaccgatt cgcgatcgct ctgcctcgcg
     5281 cgcgagatgc tcggctacgt gtcgccgcgc gcagattcga tcttcgcggt gcagggcctc
     5341 ggcgtgcatt cgatcttgct cgcgggcacc gacgaacaac gcgcgcacgt gacggcgtac
     5401 gcccgcggcg caggcatcgc ggcattcgcg ctgaccgagc cagaagcagg ctccgacgtc
     5461 gcatcgatcc agacgcgtgc tgcgccgtgc gcggagggct accgcctcga cggcgacaag
     5521 ctgttcatct cgaacctcgg catcgccgat cacgcgaccg tcgtcgccgc aacggagccg
     5581 tcgctcggcg cgggtgggct caccgcattc tggctcccgc tcgacacgcc gggcgtgacc
     5641 gtgaagccgc tcgtcgcaac ggcgccgcat ccgatcggtg cgctcgagct gcgcggtgtg
     5701 gtgctgccgg cgtcgtcgaa gatcggcgaa gtcgggcagg gcatgaagct cgcgatgctg
     5761 acgctcgatg cattccgcgt atcggtcggc gctgctgcag tgggcatggc gcggcgcgcg
     5821 atggatgaag cgatcacgtt cgtcacgcga cgcatgcagt tcggcaagct cctcagcgag
     5881 cagccgctca ttcaagcgca catcgccgac atggcagtcg acctcgacgc ggcgcggctc
     5941 ctcgtgctgc gcgctgcgca tcgcaaggac acgacgaccg atcgcgtgac gaccgaagtc
     6001 tcgatcgcga agctcggcgc caccgaggcc gcacagcgcg tcatcgatcg tgcggtccag
     6061 ctcctcggtg gacgcggcgt gatggccggc tctgtcgtcg agcatctcta tcgcgcgatc
     6121 cggccgctgc gcatctacga aggcacgagc gagatccagc gcacgatcat tggcagggct
     6181 ctcacgaagc ccggaggggc gcgaccgtca tgaccgtcac gtttcccgag gacttcaatc
     6241 tcgctgacta ctggctgttc gcacgcgagc gcgagaagaa gggcagcaag atcgcgctgc
     6301 gcttcggtga ccgttcgtgg acctacgccg aagtcgccga gcgttcgcgg ggcctcgcgc
     6361 gcgcgttgat cgaagcagga ctgcggcccg gcgagcgcgt ctacatcgtg ctgcccgata
     6421 cgcccgcatt cgtgtggtcg atcttcggga cgctcgccgc tggcggtgtc gtcgcgatgg
     6481 gcaatccaat cgcacccgtc gaggatctcg catacgtcgt cgattacgtg cgcgccgcgg
     6541 tcgtgatcac gacgccgacg gtcgcggccg cgctcgcgcc ccacatcaaa gatgtccaca
     6601 cgctgcacgc gctgctcctc gcgcccgacg cggcgaccgg cgacgacccc gaagcgccat
     6661 gcgagccgat cgtcagcgtg ccgatgtgcg aagacctcca caaggagctg cgcaagggcc
     6721 tcgcgtccga catcccgctg ccgaaaacgc atcgcgacga tcaagcgatc tggctgttca
     6781 cctccggttc gacggggcgc ccaaaagcgg cgatgcacag tcaccgcgac ttcgcgttca
     6841 acaccgaagt cttcgcgaag cagacgattg ccatgcacga ggacgacgtc tcggtctccg
     6901 tgccgcgcct gttcttcggc tacgcgacag gcacgaatct ctggttccct ttcgccgtcg
     6961 gcgcgacggt cggcctgttc agcgagcggc ccaccgccga atcgatcacc gccgcgatcc
     7021 agcgctacaa agccaccgtc gtaacgaacg tgcccacaat gctcggcaag atcctcgatc
     7081 tcgacgacga gcttcgcgcg aagggccaac gcggcctcga tctctcgagc gttcgctttc
     7141 acttcagcgc cggcgaagcg ctgcccgagc cgctgatgcg tcggttcatc gagcgctttg
     7201 gcggccagat ctacgacggc attggctcgg ccgagatgtt ccacatctac tgctcgaatc
     7261 gtccaggtga cgtgaagccg ggctcgctcg gtcgcgtcgt cgatggctac gagctgcgca
     7321 tccttcccga agaagcaaac gagcccggcg caccggagct gccgcgcggc gaaatcggcg
     7381 tgctctgggt caaaggcgac tccgtctcgc acggctactt ccgcgatcgc gacaagagct
     7441 ggaagacctt ccacggccac tggtgccgca cgggtgacct cttctcgatc gacgccgacg
     7501 gctacctgta cttccagggt cgcgccgacg acctcttcaa agtgggaggt atcttcgtcg
     7561 cgccgcgcga agtcgaagac tgcctgctcg cccacccagc agtctcggtc tgcgccgtca
     7621 tcccagccga agatggtggc ctcacgaagc ccaaagccct cgtcgtcctg cgccccgacg
     7681 cccgcgacaa agacaagcgc gcgctcgccg acgagctcaa agcccacgtc caagcgcgtc
     7741 tctcgaagca caagtatccc cgatgggtgc tgttcgtcga cgacctgccg aaaaacgatc
     7801 gcggcaaggt cgacaagaag actctgatcg agcgcgagaa gcgcggtgag ctaagctccg
     7861 actcgtgacg gagaagttcc tcgctcgcca tacgaccgca acgctcgaac aactcgtcac
     7921 gagcaagaag ggcgtgatcg cgctggtccc cgtcggctcg acggaaccgc acggcccgca
     7981 tctcggcctc ggcaccgacg tcatcatcag cgccgccgca tgcctccgcg cctgcgagct
     8041 cctcgaaaag cgcggcacga tgatcggcgt catcgccccg gccatcagct acggcgtgac
     8101 cgaatgcgcg acgggcttca aaggcgcggt ctccatccct gccgccgtgc tcacgcaata
     8161 catcgccgcg gtcgtcgacg gcctcctcgc ctccggcgtc cgccacgtct gcctcgtcaa
     8221 caaccacctc gagcccgccc acgacgccgc agtccgcgcg gtcctcgaag gtcgcgagaa
     8281 aaaagtctcc tacgcctgcc cactcacgaa aaaatgggcg cgcacgctct ccgccgaatt
     8341 caaatccggc gagtgccacg caggtcgcta cgaaacctcg atcgtgatgg ccgccgcgcc
     8401 cgagctcgtc caagaaccgc tccgcgtccg cctctcgccg gtcccagtct cgctctcgaa
     8461 gaagctcgcc gaaggcatca acaccttcga agccatgggt atggagctcg cctacgcagg
     8521 cgaccccgca tccgcctccg tcgaagaggg cgagcaactc atccagcgcc taggcgacat
     8581 ggtcgtcggc gaagttctcg aatcgctggg catcaaaccg gcataagatc gaccgatgca
     8641 cctcaccaac agcacgctca cgatcgcggt cgccctggtg tcgtcgctct atctcttcac
     8701 gcaatcgacg aaccgcacgt tcccgctcat cgcgacgatc gtagccggcg tcgagctcct
     8761 cctcacgctc ggcatcatgt ccctctccct cgcgaaatac cgcgtcgacg tgatccttcc
     8821 cgcacttctc gcgatctcgg gcttcgtttg ctggacgaag accacgtcga aaggcggagt
     8881 tacagccgcc tccgccgtcg cgctcatcgg cgcaatgcag ctcttgctcg cactccgcct
     8941 tttcagctga aataccctta tgaggctgct ctgcgccgcg ctgctactca ccgccgccgc
     9001 ttgcaccgac gacagcgatc ctgacgtccg tgtcgaacgc caagtcgcaa cgctcgacga
     9061 cgcatccgcc gccgcatggt cgcaaggtgg tgccgtcctc gtcggcgtac cgggcacgtt
     9121 cgcgttccca cagccgctct ctctccgcat ctcgatcgtc gacaacaagc aactcgacga
     9181 agagatgtcg cccacgcagc tcaagatcac gagcgcaacg ctcgccacct acggcgacgc
     9241 ctccgagctc acggtaaaga cgcagccctc ctgcgccgcg aacttctgct ccgccgagct
     9301 cgaagtctct gcgcaaggcg cgagcatgct gacgatcgag gccgacggcc ccgacggcaa
     9361 gcaaaccgag tgcttctact acgcgatcta cgaagcagac gatccggcga cggccggcgc
     9421 gacgtaccgc accgagctag agacaaagca gcgcgagtgc cgcgcgacgt actggaacta
     9481 gccggccgga gcgcgcgtgg accgcgatcc ccttactgcg atcgcgtggc agttccgcac
     9541 gaatcagttc ggtgatctcg taaagcacgt tcgcgatcac accgcgatgg ttggcatgga
     9601 gctcttggcg ctctctgggg ccaagcggtc ggccacgttg ccgacagtct ggagccagtt
     9661 gccgaggcat cccaacgtcc tcgagccact tcgcggcgac cagtctcgct ttctgttgcg
     9721 ctacgcagcg ttgaattgga cggagccgcc tttccttcgt gtcgatcaag agcggctagc
     9781 agcgacgcag ttgtttcagc tcgcgtcggt ctaccaaatg ttgttcgaaa cgaccggcaa
     9841 gaacgtgggt ttgttccgca accctctcgt gaagatcgat ctcggcggtt ttatacgcgt
     9901 ctgctttctt ccgatgaaac cgatcactgt acggccgcga gtgaccgtcg aaagcgacgt
     9961 cgaccaacaa cacctcgtgc gtttggttgg ggagtgtgcc cggcgaagcc tctccattac
    10021 agaaggcagc ttgcggtggg acattttgga gcggtgcctc acccacaata aacaacatca
    10081 atacaaatcg ctcgaggact tgatggctgc gtgccggctc gctggcatcg agtatccagt
    10141 cgagcgaaca ggtcagctct ccgcggcatg gttagagatg gagcagggtc tcggttggct
    10201 ccaacttccg cagatacgcg attatctcga tagagcgtca gatcactttg gagcgtcgct
    10261 agagctggtt cggcttgatt accgcgacgc gcagcatgag ctggttcgcg ccgttgcgag
    10321 ttggggccgt ggaacatgcg cggt
//
LOCUS       DATLBZ010000433        19042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1277308, whole genome shotgun sequence.
ACCESSION   DATLBZ010000433 DATLBZ010000000
VERSION     DATLBZ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19042
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277308"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VMZ53_21960"
     CDS             <1..558
                     /locus_tag="VMZ53_21960"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HUS31198.1"
                     /translation="KVQFAIALIHEPQLVILDEPWSGLDPINAEVLREVVEEIKASGR
                     TVLFSTHGMEQAEKICDAVCIIARDKKVLDGRLRDIKRDSVAEGVIALGFSDEASKEK
                     AAGPLEDHKLVTARRPPRPGEHADCEVILAEGVKPQQLLAILLNHDVGVRLFEVVVPT
                     LHDIFVQKVGNAAIAVRREEDDAGA"
     gene            551..1816
                     /locus_tag="VMZ53_21965"
     CDS             551..1816
                     /locus_tag="VMZ53_21965"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS31199.1"
                     /translation="MRDTWVIAKREFLERVKSKWFVVMTVLWPVLMLGMMIVPALLGG
                     QGTKGAKVEIVDHNGSLGQPMVVKLGAPPMFGGLEWDATIVPTTTTEDVLRDRLRTHA
                     INGYLIIAEDALDGGEIIYSGDNASNQTVTIGLSQAVTAVVITARGKRAGLSEMALVD
                     LVKPANVDVRYSTGEEKRGSAGIFVFLLGYMIAFLIYIVITLYGIGVMRSIVTEKSSR
                     VVELLVSATKPRAMMSGKIVGVGTAGLVQIAIWFVLAQVALSQKARILHLFGASDDAK
                     LAMMPSLSTSQLMIVMVFFLFGYLFYSAMYAAVGAMVSSEQDSQQAQMPVTFLLVIGM
                     IAIMAVTNEPRGSVAKVMTMVPFWSPMLMPVRYFLGGASAADVGLSLAILGVSTVVVS
                     RIAAKIYRVGILMYGKRPSLAELFRWIRY"
     gene            1895..3532
                     /locus_tag="VMZ53_21970"
     CDS             1895..3532
                     /locus_tag="VMZ53_21970"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HUS31200.1"
                     /translation="MRRWGFASFLSVLLIPLTASAQEADVTAGVSADQGVAPSQAIAY
                     GAMPGGLHAPTAETLPKGAVQVSTLSGLGRRTGLLGPDHKFNRAIGDLAIAFGVTDMI
                     SIGLSLDGRYDKHWGAVGDASKNLMTTSQANGDDGYVGDPHLIVRAGKSTGSLIVGGQ
                     LGVWVPGKDAPSVAGSAISVDVRGLVSLPAGPGLLSFSAGFRLDNSAKSVDDPMKLSL
                     SDRVSLGVSDYNAVFGGAQLRIPAGKAWVGIEGSLDAFVGGPGTPEMGQVAHAELARG
                     KLIFRGGLTAGYHITDTISGIVFFEATKSPGVNDAQVDDANIPLVPYEPLLAGGIGLQ
                     ARFGGPKAVKNPFDEKDCHKHNPPDCPAVKVPLLAKITGTVVDTGGKPVAGAKVSVSL
                     KNSQAEPTVTDEKGAYSLDVRIGESVDGKETIDEAGVEVNVDVSGMKPGKASLAQIAK
                     DTNTVPPITIEPVLPPGQLRGVVRSLPGGKNVEGAEIKVTPGDQKATTAADGTFTIDL
                     APGTYKISVTKNGLKTQELDVTIDPNGVAIKNIDLQK"
     gene            3498..5444
                     /locus_tag="VMZ53_21975"
     CDS             3498..5444
                     /locus_tag="VMZ53_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31201.1"
                     /translation="MLPSKTSTSRSNSRRHALTLGRLGGACGVLLLLLFAWGCKPKPT
                     AIAELTKADGPVERQAGQGAWGAATIGTKYFLGDAARTADAPAQLSLAGGRAVIKMDK
                     FTTLRFGGARGGAAKIGVELGAITLVGAGNFGLDVGNVKVEDNGEIRIVAKGQGKSSV
                     ELLVGTAQISGVDGQMVELQIGQILELGVGPIKVEAVTDAGVDAAPIDAADTTAPVAQ
                     DGTIEVKGPKGEILPPGATKWEKLAAGAAVPLTKGSKVRLGPGTTATLVANGVTLSMA
                     GGSRTAVGDDLAFGLELGSATGSVGVNAEGNVGVPGGKVGIKGSATGGGKARLDVNKN
                     GDTKIAVLEGSANLTGASGADLAMKTGESASLAKAGGIKQTAKIPDYYDMRVSIGEGS
                     FTIHDTKGATALQFFFNGKCNGGTVEMDNDPKFKTARISAGKDSANMLADGVGAYYRL
                     VCANGSVAGSGRISIRHDAGTRPLPKDPPVNPIDPDGRNYTISYQSVIPNLKIKAPAG
                     GSSYKVHMATGGADQVFDSTKPDIKVEGKSLKEATYTMFIEKDGAKVGKPTTVTISFD
                     QQAPQVYIESPQNNKPFGAEVDVRGAVLPGWTAKVLDVEIPIDARTRRFSAKVPPPTG
                     ALALAIRLSHPQRGVHYYLRRGAK"
     gene            5474..6466
                     /locus_tag="VMZ53_21980"
     CDS             5474..6466
                     /locus_tag="VMZ53_21980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31202.1"
                     /translation="MSTSRVALPCLLVAACGADPAGVGVTGSPGPYFERPMFFNQDVS
                     NVAPAANTLNVTGALRTAGGWGNRDRLTIDFGFSVLHKTPDTQMRTFTPDDAHFTTPD
                     CDMMPVPVPIPGNIEGETGYACTSRGDCHLIVVDDDAGQIYEMWKADIKSSLQFNGGC
                     LAVWDTTAAYDKTLRGDQCASADGAGFPIAPLLFNADEVASGEIDHAIRFVLPNDRVR
                     QGYVRPAVHGAYTNGVQSSDLPFYGVHMRLRADFPIDSLPSEGAKVIARAMQKYGMYH
                     ADGGEYALTAQSDRFTTAKWAGLLAENDLSSLLVEDFEVIDHGPAIEITNDCMR"
     gene            6493..8052
                     /locus_tag="VMZ53_21985"
     CDS             6493..8052
                     /locus_tag="VMZ53_21985"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HUS31203.1"
                     /translation="MRWLSRLVTILLIVGVVVLFASLVRSKMPATQVGQAFTTCASFR
                     DGTRLAVGSPVLIAGVRVGEISRISLTGKFARVDMRLINGVEIPVDSWVTKRAFSPFG
                     DSYLEILPSTPDPGAAPAVNLAPGQCIARVIEGSSTDTVLRSMNKVLPNVDEGLDTLH
                     RVAIDGRKWASGRLEDRVLGAEHWLDERQDQDPLGRANEAMARLEVSTRRAADALSAV
                     RPDVDNTLTRVADGVAQARHKITELKADMKDGLAGVRSGLDGADETIADVQEVLVSID
                     ENKNKPDYTGTLGKLVNDPELADSIEEGTESAREGLGSFGRFKSYLGLRTEFNWFSRA
                     PRFFVTAEVRARTDKFYLVELEKGPLGAFPEGQISNGPPNADYVRHQEIHDKIRFTVQ
                     FGKQFGNNFQVRGGIKESTFGFGVDVLMGQGRLKFSTDLYGGYTRVPRLKLASALEVF
                     RSVYLIAGIDDAMNTPGYFNIQKGNTNVPIQFDQVRYGRDYFLGAQLQFDDADLAMLL
                     RVYGALLVGLL"
     gene            complement(8542..8847)
                     /locus_tag="VMZ53_21990"
     CDS             complement(8542..8847)
                     /locus_tag="VMZ53_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31204.1"
                     /translation="MGIFDRLRGDQVKVHVVIRGRIGDDWKDVDEYVRVPERTTLGEF
                     VDLANQAGIPLRESLAHSPHLSDTLMLNGERCAIIDNAGRELKDGDELYLLAPITGG"
     gene            complement(8871..10898)
                     /locus_tag="VMZ53_21995"
     CDS             complement(8871..10898)
                     /locus_tag="VMZ53_21995"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HUS31205.1"
                     /translation="MGEALAPSNKGFHGRVLFVDLSTKTHRVEPIDAQVYRDYLGGYG
                     LGAWLMWKHFPVGADPFDPKACFALCSGLLTGHHAPFSSRIQIVGKSPLTNTWADSNS
                     GGSVCSHLRKAGYDAMVVTGKAEVPTLLVIKDSEVSFEPAGELWGQEIPEAFDALKDR
                     FGGKRDVGVSAIGPAGEKQLRIASVMNDRYHAFGRQGFGAIYGSKNLKAIVVAGSGEI
                     GVARPDEFKAVCKRINDEYKRDLGLFTRVFAWFLKAKKWMGFIYRSAPKTGSKLASPA
                     AAMRRAFSKRGTTLGVAISVENGDAPIKNWTGVGPKDFPIGTKSMKIDGGEVEKIVSK
                     KLSCGDCPMPCKGIVKVRSRNLTDVRRPDFETLCGFGANLLNDDLSIVTACHDACNRL
                     GIDAVSSSATLGWVAECVERGILTPADLDGIDMRWGNGEAALALTIKMGKGEGCGAWL
                     GQGVKRASEHVAKGSEEFAIHVHGGEPPYHDTRFSSLMGVGYVADPTPARHTSGSGSW
                     NETFATPFALPHAAEKKDWNVKWKGTEGKGKAQALHSNAHQVLNGLGLCMFTGLTGRL
                     PWLELVNSATGWNYTESDLLKCGERIQNLRAAFNRREGIKPADFAIPKRMLGEGDGHL
                     EAGPLKGVRVPLPVLRDDYYTAMHWNTTTGHVSKAHAETLGMADLLDGYTE"
     gene            10926..11585
                     /locus_tag="VMZ53_22000"
     CDS             10926..11585
                     /locus_tag="VMZ53_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31206.1"
                     /translation="MRMWILVVVAALGCEGKKKPAATETGSGKGSEIVAAAPADAAVV
                     APVDAAAVAVDTAIADGATAGSSDAGVAADKLDYEHILDWETIGPFKLGMDEANVVKL
                     VGAPKKKAFPVEEGATGEFVSGWEWPALGLTLEMASDRRTGPFHVRSIEIYPPSTYAT
                     SRGAKIGMSSAALPALYVRNLDEGREDPRDYLVGSPYGGLLITFKDGKAVSIFLGAMA
                     F"
     gene            11604..12152
                     /locus_tag="VMZ53_22005"
     CDS             11604..12152
                     /locus_tag="VMZ53_22005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31207.1"
                     /translation="MKYWPAILLLVTACSTKQKASITDEAYSNEGRRRELFEATLRVL
                     DENPDYVDELYKLTLRHPKTLQRQFEATARSLSDRDVAERTAAALVNHPKGLEQVMVA
                     TLEAASDKPNAANAVVDAMQKRADIAASLLVKRPAELATISKAIITRAFAEPNTKEIL
                     TKIVKEAADPKAAARSSWTPKR"
     gene            complement(12166..12894)
                     /locus_tag="VMZ53_22010"
     CDS             complement(12166..12894)
                     /locus_tag="VMZ53_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31208.1"
                     /translation="MGGGTGEIALIALSQRKLPDPLPVSRKQHGMPDEGDGPTSIALV
                     ARDRAEDPAWFTEQVVAPFADLIAADLGPQHAAIALAAEHAYVIEAQLSDPDDLGHVQ
                     AAWALAKCVCEEGATIVIDVYAARAHLGSEIARMPADRAFDIMHEVTLFFDELADGHL
                     AAWSLGLRKFGRPDIVLLDVVPDGATDAALMLRDVANTLASGERIEPGDTVASPTGRN
                     YVAEKFDPASAEMVAVEGDAILLR"
     gene            complement(12927..14684)
                     /locus_tag="VMZ53_22015"
     CDS             complement(12927..14684)
                     /locus_tag="VMZ53_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUS31209.1"
                     /translation="MTRDTIPHRVLRMAVERPSTIAYQAKVNGRWQPTTWKTYGEQIR
                     TAARSMMALGLQRGGKVAILGFNRPEWAIFDHAAMMAGGCPAGIYTTCSAEEVQYIVH
                     HSESLIMLVENAEQLAKVKAKRSELPQLKWIVMMKGAIDATGGDVLTWEDFLAKADAT
                     SDAELDKRIDSIEQSDLATLIYTSGTTGPPKGVMLSHGNLAWTSRALWQLGGQRTGEV
                     LLSYLPLSHIAEQMCSLHMPATGAHTVYFAESIEKVPENLKDARPTVFFGVPRIWEKF
                     HAAMAGKLAQTTGAKKALVDWARKVCRQVNIHRDRGEAVPKHLELQYKLADKLVISKI
                     KQAIGLDRAFEMFSGAAPIAPDVLEFFSSLDLPIHEIYGQSEDCGPTSCNISGKTKIG
                     SVGPPIPGIEVKLGDDGEILIRGPNVFLGYYKEPEATAETLKDGWLCSGDLGAFDKDG
                     FLSITGRKKEIIITAGGKNIAPKNIEALIKQSPLVGEAVVIGDRRKFLTALVTLEPTA
                     AEKLATEQIRSQIQVKIDEINQQLARVEQIKKFAILEKPFGIDSGELTPTMKIKRKVV
                     AQKYAREIEAMYSAESDAS"
     gene            complement(14693..15073)
                     /locus_tag="VMZ53_22020"
     CDS             complement(14693..15073)
                     /locus_tag="VMZ53_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31210.1"
                     /translation="MSDFQTEMNRVVTDFVNQITELARAAARDMIDEALGKGGKISIG
                     GVGRGRGRGGRGGKRTSGELDQLADRFHSFVTKHPGLRIEQINKQLGTTTKDLALPIR
                     KLLSDGALKTKGVKRSTTYFPGKN"
     gene            complement(15116..16414)
                     /locus_tag="VMZ53_22025"
     CDS             complement(15116..16414)
                     /locus_tag="VMZ53_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31211.1"
                     /translation="MRAWTLAVVLSSSLLGCTPDYPPPNPDTPSPPTSNDPAFAIDGL
                     KDWYVMPDAAQPGTNQMTFIVKAPSGSDYVDAYIPGLGGHRLHEQSDGFALDINIEGV
                     EVGTHDILFTANGSTTAFAKATFHRSMAYYVLVSTDYDFSDPGKQAIIYMDSLHTNHP
                     GMLITHFWAPYTYTDPVVTPARRDELDVWIKKQRDEMHDEIALHIHPWCHFVTSAGLT
                     CITNQSTVYPDDMSGYTIKLAAYGRQDMGTLLQRAHAIFNERGLGTPVTFRAGGWTAD
                     VQTLLALDDNGYVADTSALNWAKIEEWNGKELYTWNQTNWSQIGDTSQPYHPSTSDPQ
                     ASVPGANMRMLEVPDNGVMIDYVSLEEMNGLFDANWDGQPLAAPKTLMMGFHPAGGFS
                     PSEYGRVDNFLKYADMHLGSAGTGPVVYATLSQVSAVFPP"
     gene            16471..16986
                     /locus_tag="VMZ53_22030"
     CDS             16471..16986
                     /locus_tag="VMZ53_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31212.1"
                     /translation="MRLIVLVFFAAACGGATAESPLTVQLGLNHDQTKQMLAQHQFCL
                     ETSSSTVAATQQKQVYPRCNRPAAEHGEAWVVAMYDGDKLVELRRFESYGDDARAVER
                     WNELITERMKTSQPDDAALQQIKEQGMLEPGTRSVKAFRGEPGTVVGVYLLTPSPPAN
                     ANVLEKVTYLK"
     gene            complement(17047..17925)
                     /locus_tag="VMZ53_22035"
     CDS             complement(17047..17925)
                     /locus_tag="VMZ53_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31213.1"
                     /translation="MRKTLALLLSLPLIGCVVGDGTMTGDDGTGGDDGTGSGSGSGSG
                     SGPTGGISGHITASTDWTGNVLISGITTIDPGVTVTIAAGTTVSLKGQASLQVNGILD
                     AQGTSASKVTLKPEGMGWNGIMVNAGGELKFKFVDQAGGNITTSGTGKATIADSHLHH
                     AAGDFLMMNGGTMEVSYSTVGMEPGQTDTTHCQLHFNPGGNVIKVTHSNISTSSYGLM
                     FYGGMNADFQYNNWFSNTTDVDTQQGSPVSGNFSNGWFEKGNPTGAGITATNMATARL
                     AACTGANDAMCAGARP"
     gene            complement(18065..>19042)
                     /locus_tag="VMZ53_22040"
     CDS             complement(18065..>19042)
                     /locus_tag="VMZ53_22040"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUS31214.1"
                     /translation="ALETKAFCGSSVVLGVGNVASGFHTYKLVTGQAWTLDPLNPAFA
                     YDDFSGNADGKNSVLNTPDSGRGHLAYLGEACSAKLGNCRDVTAYLPAGYDAPATAST
                     KYPVLFMHDGQNVWDDHDCCFGHTGWEVNVTLDTEIAAKRVAPVVVIAADMTNKRLDE
                     YGLDATATANFIDFQVNELQPKFVAKVRADGRVYVAGSSLGGLISMELALAHPQTYAG
                     IACLSSSFWVGQDEGTAVRDQLRGASKMPVSIYLDSGGALASNADSAADTAEVRDMLV
                     NLGWQRGDSPSCASGPNALCYRLEPGAQHDELAWKARAWRFLRFLFPAS"
BASE COUNT         3285 a         6099 c         6276 g         3382 t
ORIGIN      
        1 aaggtccagt tcgcgatcgc gctcattcac gagccgcagc tcgtgatcct cgacgagcca
       61 tggagcggtc tcgatccgat caacgccgag gtgctgcgcg aggtcgtcga agagatcaaa
      121 gcgtcgggcc gcacggtctt gttctcgacg cacggcatgg aacaagccga gaagatctgc
      181 gacgcggtct gcatcatcgc gcgcgacaag aaggtcctcg acggcaggct gcgtgacatc
      241 aagcgcgact ccgtcgccga aggcgtgatc gcgctcggct tctccgacga ggcatcgaag
      301 gagaaagcgg ccggcccact cgaggatcac aagctcgtga ccgcgcgtcg tccaccgcgc
      361 ccgggcgagc acgccgattg cgaagtgatc ctcgccgagg gcgtgaagcc gcaacagctg
      421 ctcgcgatcc tgctcaacca cgacgtcggc gtgcggctgt tcgaggtcgt cgtcccgacg
      481 ctgcacgata tcttcgtgca gaaggtcggc aacgccgcga tcgccgtgcg tcgcgaagag
      541 gatgacgccg gtgcgtgata cctgggtcat cgcgaagcgc gagtttctcg agcgcgtgaa
      601 gagcaagtgg ttcgtcgtca tgacggtgct ctggcccgtc ttgatgctcg gcatgatgat
      661 cgttcccgcg ctgctcggcg gccagggaac gaagggcgcg aaggtcgaga tcgtcgacca
      721 caacggctcg cttggtcagc cgatggtcgt gaagctcgga gcgcccccga tgttcggcgg
      781 cctcgagtgg gacgcgacga tcgttccgac gacgacgacc gaggacgtgc tgcgcgatcg
      841 gctgcgcacg cacgcgatca acggctacct gatcatcgcc gaggacgcgc tcgacggcgg
      901 cgagatcatc tactcgggag acaacgcgag taaccagacg gtgacgatcg gtctgtccca
      961 agcggtcacc gcggtcgtga tcacggctcg tggtaagcgc gcgggcctct ccgagatggc
     1021 cctggtcgat ctcgtgaagc cggcgaacgt cgacgtgcgt tactcgaccg gcgaagagaa
     1081 gcgcggcagc gctggcatct ttgtcttttt gctcggctac atgatcgcgt ttctcatcta
     1141 catcgtgatc acgctgtatg gcatcggcgt gatgcgatcg atcgtgaccg agaagtcgag
     1201 tcgcgtcgtc gagctgctcg tgtctgccac caagccgcgc gcgatgatgt ctggaaagat
     1261 cgtgggcgtc ggcactgcgg gcctcgtcca gatcgcgatc tggttcgtgc tcgcgcaggt
     1321 cgcgctgtcc cagaaagcca ggatcctcca cctgttcggc gcgagcgacg acgcgaagct
     1381 cgcgatgatg ccgtcgctct cgacgtcgca gctgatgatc gtcatggtgt tcttcctgtt
     1441 cggctacctg ttctactcgg ccatgtatgc cgcagtaggc gcgatggtct cgtcggagca
     1501 ggattcgcag caggcccaga tgccggtcac gttcctgttg gtgatcggga tgatcgcgat
     1561 catggcggtc accaacgagc ctcgtggaag cgtcgcgaag gtcatgacga tggtgccgtt
     1621 ctggtcaccg atgctcatgc cggttcgcta cttcctcggc ggtgcgagtg ccgcagatgt
     1681 gggcctgtcg ctcgccatct tgggcgtctc gaccgtggtc gtctcccgca tcgcggcgaa
     1741 gatctaccgc gtgggcatcc tgatgtatgg caagcgcccc tcgctcgccg agctgtttcg
     1801 ctggattcgc tactgaccgc ttgccttatc gaagtagcgt aaaaccctgc gccggagtgg
     1861 aaatcacctc ggtggacccc ttacaatttt tgcaatgcgt cggtggggat tcgcatcgtt
     1921 tctctccgtg ctgctaattc cgctcacggc gtccgctcaa gaggctgacg tcaccgccgg
     1981 agtcagcgcg gaccaaggtg ttgcaccttc gcaagccatc gcctacggcg cgatgcctgg
     2041 cggcctgcac gcgccgactg ccgagacgct gcccaagggc gccgttcagg tgtcgacgtt
     2101 gtcgggactc gggcggcgga ccggcttgct cggtccagac cacaagttca accgcgccat
     2161 cggcgatctc gcgatcgcgt tcggcgtgac ggacatgatc tcgatcgggc tctcgctcga
     2221 cggccgctac gacaagcact ggggcgccgt cggcgacgcg tcgaagaacc tcatgacgac
     2281 ctcgcaggcc aacggcgacg atggctacgt cggggaccct cacctcatcg ttcgcgccgg
     2341 caagagcacg ggcagcttga tcgtcggtgg tcagctcggc gtgtgggtgc ctggcaaaga
     2401 cgctccgtcg gtcgcgggct ccgcgatctc cgtcgatgtc cgcggcctcg tctcgctccc
     2461 tgcgggtccg ggcctgctgt cgttctcggc cggtttccgc ctcgacaaca gtgcgaagag
     2521 cgtcgacgac ccgatgaagc tctcgctctc cgatcgcgtc tcgctcggcg tcagcgatta
     2581 caacgcggtg ttcggcggcg cgcagctgcg cattcctgcc ggcaaagcgt gggtcggcat
     2641 cgaaggctcg ctcgacgcgt tcgtcggcgg ccccggcacg ccggagatgg gccaagtcgc
     2701 ccacgccgag ctcgcgcgcg gcaagctgat cttccgcggc ggcctgacgg ctggctatca
     2761 catcaccgat acgatctccg gcatcgtctt cttcgaggcg acgaagtctc cgggcgtcaa
     2821 cgacgcgcaa gtcgacgacg caaacatccc gctcgttccg tacgagccgc tcctcgccgg
     2881 cggcatcggc ctccaagcgc ggtttggcgg accgaaggcc gtgaagaatc cgttcgacga
     2941 gaaggattgc cacaagcaca acccgccgga ttgccctgcg gtcaaggtgc cgctgctcgc
     3001 gaagatcacg ggcaccgtcg tcgacaccgg tggcaagccg gtcgccggcg cgaaggtctc
     3061 ggtgagcctc aagaactcgc aggcggagcc gacggttacc gacgagaagg gcgcgtactc
     3121 gctcgacgtt cgcattggcg aatcggtcga cggcaaggaa acgatcgacg aagccggtgt
     3181 tgaagtgaac gtcgacgtca gcggcatgaa gccgggcaag gcatcgctcg cgcagatcgc
     3241 gaaggacacg aacacggtgc cgccgatcac gatcgagccg gtgctgccgc cgggtcagct
     3301 acgcggtgtc gttcgctcgc tgccgggtgg caagaatgtc gaaggcgccg agatcaaagt
     3361 gacgcctggc gatcagaagg cgaccacggc ggccgacggt acgttcacca tcgacctcgc
     3421 ccctggcacg tacaagatca gcgtcacgaa gaacggtttg aagacgcagg agctcgacgt
     3481 cacgatcgat ccgaacggtg ttgccatcaa aaacatcgac ctccagaagt aacagccgcc
     3541 gtcacgcgct cacgctgggg agactcggcg gcgcgtgcgg cgtcctgctg ctgcttttgt
     3601 tcgcgtgggg ctgcaagccg aagcccaccg cgatcgccga gctgacaaaa gccgacggcc
     3661 cggtcgagcg ccaagcgggc cagggcgcgt ggggcgcggc caccatcggg acgaagtatt
     3721 tcctcggtga tgcggcgcgc acggccgatg cgccagcgca gctctcactc gcgggtggac
     3781 gtgccgtcat caagatggac aagttcacga cgcttcgctt cggcggtgcg cgtggaggtg
     3841 ccgcgaagat cggcgtcgag ctcggcgcga tcacgctcgt cggcgccggc aacttcggcc
     3901 tcgatgtcgg caacgtgaag gtcgaggaca acggcgagat tcgcatcgtc gcgaaggggc
     3961 agggcaagag ctctgtcgag ctgctcgtcg gtaccgcaca gatttcaggc gtcgacggcc
     4021 agatggtcga gctgcagatc ggtcagatcc tggagctcgg cgtggggccg atcaaagtcg
     4081 aagcggtcac cgatgcaggc gtcgatgcgg cgccgatcga tgcagccgac acgacggcgc
     4141 cggtcgcgca agacggcacg atcgaagtca aaggccccaa aggcgagatc ctgccgccgg
     4201 gcgcgacgaa gtgggagaag ctcgcggcgg gtgcagcggt tccgctgacg aagggctcga
     4261 aggttcggct cggtccgggg acgaccgcga cgctcgtcgc caacggcgtc acgctttcga
     4321 tggctggtgg ctcgcgtacg gcggtcggcg atgacctcgc attcgggctc gagctcggca
     4381 gcgcgacggg ctcggtcggc gtcaacgcgg aaggcaacgt cggcgttccg ggcggcaaag
     4441 tcggcatcaa gggctcggcg acgggaggcg gcaaagcgcg cctcgacgtc aacaagaacg
     4501 gcgataccaa gatcgcggtg ctcgaaggct cggcgaatct gaccggtgcg tcgggcgcgg
     4561 atctcgcgat gaagaccggc gagagcgcgt cgctcgcgaa ggccggtggc atcaagcaga
     4621 cggcgaagat tcccgactac tacgatatgc gcgtctcgat cggcgagggc tcgttcacga
     4681 tccacgacac gaagggcgcg acggcgctgc agttcttctt caacggcaag tgcaacggcg
     4741 gcacggtcga gatggacaac gatccgaagt tcaagaccgc gcgcatctcg gccggcaagg
     4801 acagcgcgaa catgctcgcc gatggcgtcg gcgcgtacta ccggctcgtc tgcgcgaacg
     4861 gctccgtcgc aggctcgggg cgcatctcga ttcgtcacga cgccggcacc cggccgctgc
     4921 cgaaggatcc gccggtcaac ccgatcgatc cggacggccg caattacacg atctcgtatc
     4981 agtcggtcat cccgaacttg aagatcaagg cgcccgcagg tggctcgagc tacaaggtgc
     5041 acatggcgac gggcggcgcc gatcaggtgt tcgactcgac gaagccagac atcaaggtcg
     5101 agggcaagag cctcaaagaa gcgacctata cgatgttcat cgagaaggac ggcgcgaagg
     5161 tcggcaagcc gacgaccgtg acgatcagct tcgaccagca ggcgccgcag gtctacatcg
     5221 agtcgccgca gaacaataag ccgttcggcg ccgaggtcga cgtgcgcggc gccgtgcttc
     5281 ccggctggac cgcgaaggtt ctcgacgtcg agattccgat cgatgcccgc acgcgccggt
     5341 tcagcgcgaa ggtgccaccg cccacgggcg cgctcgcgct cgctattcgc ctctcgcacc
     5401 cgcagcgcgg cgtgcattac tacctacgac gcggcgcgaa gtaggaacgc cacttgctcc
     5461 tgtcacggga gctatgtcga cctccagagt tgcgttgccg tgcctcctcg tcgctgcgtg
     5521 cggcgccgac ccagcgggcg taggcgtcac aggctcgccg gggccctatt tcgagcgtcc
     5581 gatgttcttc aaccaggacg tctcgaacgt ggcgccggcg gcgaatacgt tgaacgtgac
     5641 cggagcgctg cgcaccgccg gcggttgggg caatcgcgat cgcttgacga tcgacttcgg
     5701 cttctcggtc ctgcacaaga cgcccgacac gcagatgcga acgttcacgc cggacgacgc
     5761 gcacttcacg acgcccgact gcgacatgat gccggtcccc gtgccgattc ccggcaacat
     5821 cgagggcgag acgggctacg cgtgcacgag ccgcggtgac tgccacctga tcgtcgtcga
     5881 cgatgacgcg ggccagatct acgagatgtg gaaggccgac atcaagtcgt cgcttcagtt
     5941 caacggcggc tgcctcgcgg tctgggacac gacggccgca tacgacaaga cgctgcgcgg
     6001 tgatcagtgc gcgagcgcgg acggtgcggg cttcccgatc gcgccgctgc tcttcaacgc
     6061 cgacgaggtc gcgagcggcg agatcgatca cgcgatccga ttcgtgctgc ccaacgatcg
     6121 cgttcgtcag ggctacgtcc gcccagcggt ccacggcgcg tacaccaacg gcgtccagtc
     6181 gtcggacctg ccgttctatg gcgtccacat gcgcctgcgc gccgacttcc cgatcgactc
     6241 gctgccgagc gaaggcgcga aggtgatcgc gcgcgcgatg cagaagtacg gcatgtatca
     6301 cgccgacggt ggcgagtacg cgctgaccgc gcagagcgat cgcttcacga ccgccaagtg
     6361 ggcaggactg ctcgccgaga acgacctcag ctcgctgctc gtcgaggact tcgaggtcat
     6421 cgaccacggt cctgcgatcg agatcacgaa cgattgcatg cgatagcgcg gcgacgggtt
     6481 tagactcggg acgtgcgctg gctctcccga ctcgtcacga tcctgctgat cgtcggcgtc
     6541 gtcgtgctgt tcgcctcgct cgtgcgttcg aagatgccgg cgacgcaagt cggccaagca
     6601 ttcacgacct gcgcatcgtt tcgcgacggc acgcgcctcg cggtcggctc accggtgctc
     6661 atcgcaggcg ttcgcgtcgg cgaaatctcg cggatctcgc tgaccggcaa gttcgcgcgc
     6721 gtcgacatgc ggctcatcaa cggagtcgag atccccgtcg attcctgggt gaccaaacgc
     6781 gcgttctcgc cattcggcga cagctacctc gagatcctcc cttccacccc agacccgggc
     6841 gcggcccccg cggtcaatct cgcgcccggc caatgcatcg ctcgcgtgat cgaaggctcg
     6901 tcgacggaca cggtcctgcg gtcgatgaac aaggtcctgc cgaacgtcga cgaaggcctc
     6961 gacaccctgc accgcgtcgc gatcgacgga cgcaaatggg cgagcggccg cctcgaagac
     7021 cgcgtcctcg gtgccgagca ctggctcgac gaacgccaag accaagatcc gctgggccgc
     7081 gcaaacgaag cgatggctcg cctcgaagtc tccacccgcc gcgccgccga cgcactctcc
     7141 gccgtgcgtc ccgacgtcga caacacgctc acgcgcgtgg ccgacggtgt agcgcaagca
     7201 cgccacaaga tcaccgagct caaagccgac atgaaagacg gcctcgcggg tgtccgctcg
     7261 ggcctcgacg gcgccgacga aaccatcgcc gacgtccaag aagtgctcgt ttcgatcgac
     7321 gagaacaaga acaagcccga ctacacgggc actctcggca agctcgtcaa cgatccagag
     7381 ctcgccgatt cgatcgaaga aggcaccgaa tcggcgcgcg aaggcctcgg cagcttcggc
     7441 cgcttcaagt cctacttggg cctgcgcacg gagttcaact ggttctctcg cgcaccgcgc
     7501 ttcttcgtca cggccgaagt ccgcgctcgc accgacaagt tctacctcgt cgagctcgag
     7561 aaaggcccgc tgggcgcatt cccggaaggc caaatctcca acggcccgcc gaacgccgac
     7621 tacgtccgcc accaagagat ccacgacaag attcgcttca ccgtccaatt cggcaaacag
     7681 ttcggcaaca acttccaagt ccgcggcggc atcaaggagt cgaccttcgg cttcggtgtc
     7741 gacgtgctga tgggccaagg ccgcctgaaa ttctcgacgg acctgtacgg cggttacacg
     7801 cgcgtgcccc gcctcaagct cgcgagtgcg ctcgaagtgt tccgctccgt ctatctcatc
     7861 gcgggcatcg atgatgcgat gaacacgccg ggctacttca acatccagaa gggcaacacg
     7921 aacgttccga tccagttcga ccaagtgcgg tacggacgag actacttcct cggcgcgcag
     7981 ctgcagttcg atgacgcgga tctggcgatg ctgctgcgcg tgtacggcgc actgctcgtc
     8041 ggcctgctgt agcgccgctc ggtcggctgc cgctccgtcg ctcggtgcgt cgcctcggtc
     8101 ggctgccgct ccgtgatccg tgcgtcgctc caccgtggcg tgtatttcgc ggatcggagg
     8161 cgatcgccgg tcttcccaga tggaacacaa agacgcagag aaggcagagg acgcagaaag
     8221 gggtcgagca gggcgcacgg gcagggcgtg tgctcatcgt tcgcacttcg ctacggcagg
     8281 gcgtgtgccc ggagttaacg caggggcaga gcgcagggat cgcagggatc gcagaaatcg
     8341 caggcccaga gtgaccagca caagccctgc ccgtgcgtcc cccccaaccc ttttctgcgg
     8401 cctctgcctc ctttgcggct ttgcgttcca tccgggaaca gggcgaaggg ggaacagggc
     8461 gaagggcgaa cagcgcgaac ggggcgcacg aaatgtccgc gccgtggcgg cacacgaagc
     8521 cgcggagcgg gggcgcggcg actacccgcc ggtgatcggc gcgagcaggt agagctcgtc
     8581 gccgtctttg agctcgcgac ctgcgttgtc gatgatcgcg cagcgctcgc cgttgagcat
     8641 cagcgtgtcg gagaggtgcg gcgagtgagc gagcgattcg cgcagcggga tgccagcttg
     8701 gttcgcgagg tcgacgaact cgccgagcgt tgtgcgttcg ggaacgcgca cgtattcgtc
     8761 gacgtctttc cagtcgtcac cgatgcggcc gcggatgacg acgtggactt tgacttggtc
     8821 gccgcgcagt cgatcgaaga tgcccatcgc gtgcgtactc cgttggctcg ttattcggtg
     8881 tagccgtcga ggaggtcggc catgccgagg gtttcggcgt gggctttgct gacgtgaccc
     8941 gtcgtcgtgt tccagtgcat cgccgtgtag tagtcgtcgc ggaggacggg gagcggcacg
     9001 cgcacgcctt tgagcgggcc tgcttcgagg tggccatcac cttcgccgag catgcgtttg
     9061 gggatcgcga agtccgcggg cttgatgcct tcacggcggt tgaatgcggc gcggaggttt
     9121 tggatccgct cgccgcattt taggaggtcg gactccgtgt agttccagcc cgtggcgctg
     9181 ttgacgagct cgagccaggg gaggcggccg gtgaggcccg tgaacatgca gaggccgagg
     9241 ccgttgagaa cttggtgggc gttcgagtgg agggcttggg ctttgccttt gccttcggtg
     9301 cccttccatt tgacgttcca gtccttcttc tcggcggcgt gcgggagggc gaacggcgtg
     9361 gcgaaggtct cgttccacga accggacccc gacgtgtgcc gcgcaggcgt cggatcggcg
     9421 acgtagccaa cgcccatcag cgagctgaag cgagtgtcgt gatacggggg ctcgccgccg
     9481 tggacgtgga tcgcgaactc ttcgctgcct ttggcgacgt gctcgctcgc gcgcttgacg
     9541 ccttggccga gccacgcgcc gcagccttcg cccttgccca tcttgatcgt gagcgcgagc
     9601 gcggcttcgc cgttgcccca gcgcatgtcg atgccgtcga ggtcggcggg cgtgaggatg
     9661 ccgcgctcta cgcattcggc gacccagcca agcgtcgccg acgaggagac cgcgtcgatg
     9721 ccgaggcggt tgcacgcgtc gtggcacgcg gtgacgatcg agaggtcgtc gttcaggaga
     9781 tttgcgccga agccgcacag tgtctcgaag tccgggcgcc ggacgtccgt caggttgcgc
     9841 gacctgacct tgacgatgcc cttgcatggc atcgggcagt cgccgcacga gagcttcttc
     9901 gagacgatct tctcgacttc gccaccatcg atcttcatcg acttggtgcc gatcgggaag
     9961 tccttcgggc cgacgcccgt ccaattcttg atcggcgcgt cgccgttctc gacggagatt
    10021 gcgacgccga gcgtcgtgcc gcgtttcgag aatgcgcggc gcatcgcggc ggcaggggag
    10081 gcgagcttgc tgccggtctt cggcgcggag cggtagatga aacccatcca cttcttcgcc
    10141 ttgaggaacc acgcgaacac gcgcgtgaac aggccgaggt cgcgcttgta ctcgtcgttg
    10201 atgcgtttgc agacggcttt gaactcgtcg gggcgcgcga cgccgatctc gccggagccg
    10261 gcgacgacga tcgctttgag gttcttgctg ccgtagatcg cgccgaagcc ttggcgaccg
    10321 aacgcgtggt agcggtcgtt catcacgctc gcgatgcgca gctgcttttc gccggcgggg
    10381 ccgatcgcgg agacgccgac atcgcgcttg ccgccgaagc gatctttgag cgcgtcgaac
    10441 gcttcgggga tttcttggcc ccagagctcg cccgcgggct cgaacgagac ctcgctgtct
    10501 ttgatgacga gcagcgtcgg cacttctgct ttgcccgtga cgaccatcgc gtcgtagccg
    10561 gctttgcgga ggtggctgca cacgctgcca cccgagttag agtcggccca cgtgttcgtc
    10621 agcggcgact tgcccacgat ctggatgcgc gacgaaaacg gtgcatggtg gcccgtgagc
    10681 aggccggagc acagcgcgaa gcaagccttg ggatcgaacg gatcggcgcc gacggggaag
    10741 tgcttccaca tgagccacgc gccgaggccg tagccgccga ggtagtcgcg gtagacctgc
    10801 gcgtcgatcg gctctacgcg gtgagtcttc gtcgagagat cgacgaacag gacccgccca
    10861 tgaaagccct tgttcgaagg agctagcgct tcacccatat cgggcgagca tactatcgtc
    10921 gcgcgatgcg gatgtggatt ctcgtcgtgg ttgcggccct gggctgcgaa ggcaagaaga
    10981 agccggcagc gaccgaaaca ggcagcggca agggctccga gatcgtcgca gctgcgcctg
    11041 cggatgcggc ggtggtagcg ccggtcgatg cggctgccgt cgcggtcgac actgcgatcg
    11101 ccgatggtgc gaccgcaggc agctcggatg cgggtgtcgc agcggacaag ctggactacg
    11161 agcacatcct cgactgggag acgatcggcc cgttcaagct cggcatggac gaagcgaacg
    11221 tcgtaaagct cgtgggcgcg ccaaagaaga aggcgtttcc cgtcgaggaa ggcgcgaccg
    11281 gcgaattcgt gagcggctgg gagtggcccg cgctgggact cacgctcgag atggcgagcg
    11341 acagacgcac cggaccgttt cacgtgcggt cgatcgaaat ctatccgccg agcacgtacg
    11401 cgacgagccg gggcgcgaag atcggaatgt catccgccgc gctgccggcg ctgtacgtgc
    11461 gcaacctcga cgagggccgc gaggatccga gggactatct cgtcggctcg ccgtacggcg
    11521 ggttgttgat cacgttcaag gatggcaagg ccgtctcgat cttcctcggt gcgatggcgt
    11581 tctgattgca cgaggtcgcc ggcatgaagt actggccggc catcctgctc ctcgtcacgg
    11641 cgtgcagcac gaagcagaag gcgtcgatca ccgacgaggc atattcgaac gagggccgtc
    11701 gccgcgagct gttcgaggcc acgctgcgcg ttctcgacga gaatccggac tacgtggacg
    11761 agctctacaa gctgacgctt cgtcatccaa agacgctgca gcggcagttc gaggcgaccg
    11821 cgcgcagtct gagcgatcga gacgtcgcag agcgcactgc ggccgcgctc gtgaaccatc
    11881 ccaagggcct cgagcaagtc atggtggcga cgctcgaagc ggcgagcgac aaaccgaacg
    11941 ccgcaaacgc cgtcgtcgat gcgatgcaga agcgcgccga tatcgcggcg agcttgctcg
    12001 tgaagcggcc cgcggagctg gcaacgatct cgaaggcgat catcacgcgg gcattcgccg
    12061 agccgaatac gaaagagatc ctgacgaaga tcgtgaagga ggctgccgac cccaaggcgg
    12121 ctgcacgcag ctcgtggacg ccgaagcgct agcggcgcgc ggtcgtcagc gcagcaggat
    12181 cgcgtccccc tcgacggcga ccatctcggc gctcgccgga tcgaacttct cggcgacgta
    12241 gttgcggccc gtcggtgacg cgaccgtatc gcccggctcg atgcgttcgc ccgaggcgag
    12301 cgtgtttgcg acatctcgca gcatcagcgc cgcgtcggtt gcaccgtcgg gcacgacatc
    12361 gagcagcacg atgtcgggac ggccgaactt gcgcagtccg agcgaccacg ccgcgaggtg
    12421 gccgtcagcg agctcgtcga agaacagcgt cacctcgtgc atgatgtcga acgcgcggtc
    12481 tgcgggcatg cgtgcgatct cgctgccgag atgcgcgcgc gccgcgtaga cgtcgatcac
    12541 gatcgtggcg ccctcttcgc agacgcactt cgcgagcgcc cacgccgctt gcacgtggcc
    12601 gaggtcgtcc ggatcggaga gctgcgcttc gatcacgtat gcgtgctcgg cggcgagcgc
    12661 gatcgcggcg tgttgcgggc cgaggtccgc ggcgatgaga tcggcgaatg gcgccacgac
    12721 ttgctcggtg aaccacgctg gatcctcggc gcggtcgcgt gcgacgagcg cgatcgacgt
    12781 cgggccgtcc ccttcgtcgg gcatgccgtg ctgcttgcgc gagaccggaa gcggatcggg
    12841 cagcttgcgc tgtgacagcg cgatcaaggc gatctcgccc gtcccgccac ccacgacgaa
    12901 attctcgcgc ggccaggtcg ccaccactag ctggcgtcgc tctcggcgga gtacatcgct
    12961 tcgatctcgc gcgcgtactt ctgggcgacg accttgcgct tgatcttcat cgtcggcgtg
    13021 agctcacccg agtcgatgcc gaacggcttc tcgaggatcg cgaacttctt gatctgctcg
    13081 acgcgtgcga gttgctggtt gatctcgtcg atcttcactt ggatctgcga ccggatctgc
    13141 tccgtcgcca gcttctcggc agccgtcggc tcgagcgtga cgagcgcggt gaggaacttg
    13201 cggcggtcac cgatcacgac cgcttcaccg acgagcggcg actgcttgat caacgcttcg
    13261 atgttcttcg gcgcgatgtt cttgccgccg gcggtgatga tgatttcctt cttgcgaccc
    13321 gtgatcgaga ggaagccgtc cttgtcgaac gcaccgagat cgccagagca gagccagccg
    13381 tctttgagcg tctcggccgt cgcctcgggc tccttgtagt agccgagaaa cacgttcggg
    13441 ccgcggatca ggatctcgcc gtcgtcgccg agcttcacct cgatgcccgg aatcggcggg
    13501 ccgacgctgc cgatcttcgt cttgcctgag atgttgcacg acgtcgggcc gcaatcttcg
    13561 gactggccgt agatctcgtg aatcggcagg tcgaggctcg agaagaactc gagcacgtcc
    13621 ggcgcgattg gcgcggcgcc cgagaacatc tcgaacgcgc gatcgagacc gatcgcttgc
    13681 ttgatcttcg agatgacgag cttgtccgcg agcttgtact gcagctcgag gtgcttcggc
    13741 acggcctcgc cgcgatcgcg atggatgttg acctggcggc acaccttgcg cgcccaatcg
    13801 acgagcgcct tcttcgcgcc ggtcgtctgc gcgagcttgc cggccatcgc ggcgtggaac
    13861 ttctcccaga tgcgcggcac gccgaagaac accgtcggac gcgcgtcctt cagattctcg
    13921 ggcaccttct cgatcgactc cgcgaagtag accgtgtgcg caccggttgc cggcatgtgc
    13981 agcgagcaca tctgctcggc gatgtgcgag agcggcagat acgacagcag cacttcgccg
    14041 gtgcgctggc caccgagctg ccacagcgcg cgcgacgtcc acgcgagatt gccgtgcgac
    14101 agcatcacgc ccttcggcgg gcccgtcgtt cccgacgtgt agatcagcgt ggcgagatcg
    14161 gactgctcga tcgaatcgat gcgcttgtcg agctcggcgt cgctcgtcgc gtcggccttc
    14221 gcgaggaagt cttcccacgt gagcacatcg ccgccggtcg cgtcgatcgc gcccttcatc
    14281 atcacgatcc acttcagctg tggaagctcg ctgcgcttcg ccttcacctt cgcgagctgc
    14341 tcggcattct cgacgagcat gatcagcgat tcagagtgat gaacgatgta ttggacttct
    14401 tctgccgagc acgtcgtgta aatgcccgcc ggacagccgc ccgccatcat cgctgcgtga
    14461 tcgaagatcg cccactcggg acgattgaac ccgagaatgg ccaccttgcc gccgcgctgg
    14521 agcccgagcg ccatcatcga gcgcgccgcg gtgcggattt gctcgccgta cgtcttccac
    14581 gtcgtcggtt gccagcgccc gttgaccttt gcttggtacg cgatcgtcga aggccgttcg
    14641 accgccatgc gcagcacgcg atgaggaatg gtgtcgcgag tcatctgggt cgtcagttct
    14701 tcccggggaa gtaggtggtc gagcgcttga cgcccttggt cttcagcgcc ccgtcggaga
    14761 gcagcttgcg aatcggcagc gcgaggtcct tcgtggtcgt gccgagctgc ttgttgatct
    14821 gctcgatgcg cagaccaggg tgcttcgtga cgaacgagtg aaagcgatcc gccagttggt
    14881 cgagctcgcc cgatgtccgc ttacccccgc ggcctccgcg tccacgtcca cgtccgacgc
    14941 ctccgatcga aatcttgccg cccttaccga gcgcttcgtc gatcatgtcg cgagctgcgg
    15001 cacgcgcgag ctcggtgatc tggttgacga agtcggtgac gactcgattc atctcggttt
    15061 ggaaatcact cattgaggag cgtgcttatc caattaccga atcaaacaca actcatcacg
    15121 gagggaatac cgcgctcact tggctcagag tcgcgtacac gacaggtccg gttccagccg
    15181 atccgagatg catgtcggcg tacttgagga agttgtcgac gcgcccgtac tcgctcggcg
    15241 agaagccacc cgcgggatgg aatcccatca tcagcgtctt cggtgcggcg agcggttggc
    15301 cgtcccagtt cgcatcgaac agaccgttca tctcttcgag cgatacgtaa tcgatcatca
    15361 cgccgttgtc cggcacttcg agcatgcgca tgttcgcgcc gggcacgctc gcctgaggat
    15421 ccgatgtgct cggatggtac ggctggctcg tgtcaccgat ctgcgaccag ttggtctggt
    15481 tccacgtgta cagctccttg ccgttccact cctcgatctt cgcccagttc agcgccgacg
    15541 tatcggcgac gtagccgttg tcgtcgagcg cgagcagcgt ctgcacgtcg gccgtccagc
    15601 cacccgcacg gaacgtcacg ggcgtgccga gaccgcgctc gttgaagatc gcgtgagcgc
    15661 gctgcagcag cgtgcccatg tcttggcggc cgtacgcggc gagcttgatc gtgtagccgc
    15721 tcatatcgtc cggatacaca gtcgactgat tcgtgatgca ggtgaggcct gcgctcgtga
    15781 cgaagtggca ccacgggtgg atgtgcaacg cgatctcgtc gtgcatctcg tcgcgctgct
    15841 tcttgatcca cacgtcgagc tcgtcgcggc gcgccggcgt cacgaccgga tccgtatacg
    15901 tgtatggcgc ccaaaaatgg gtgatcaaca ttccaggatg gtttgtgtgc aaactatcca
    15961 tgtagatgat tgcttgcttt ccaggatccg aaaaatcgta atccgtggag acgagcacgt
    16021 agtacgccat gctgcgatgg aacgtcgcct tcgcaaaggc ggtcgtcgat ccgttcgcgg
    16081 tgaacagaat gtcgtgcgtg ccgacctcga cgccctcgat gttgatgtcg agcgcgaagc
    16141 cgtcggactg ctcgtgcagg cgatgcccac cgaggcccgg aatgtacgca tcgacgtagt
    16201 cgctgcccga cggcgctttg acgatgaacg tcatctgatt cgtgcccggc tgcgcggcat
    16261 cgggcatcac gtaccagtct ttgagaccgt caatcgcgaa cgcaggatcg ttgctagtcg
    16321 gcgggctcgg cgtgtcggga ttgggcggcg gataatccgg cgtacacccc aaaagactcg
    16381 acgagagaac gacagcaagc gtccaagccc tcatggcgcg ggaccctaac acgaagaaat
    16441 cggggccgat catggtagtt ttcgcgttcc atgaggctga ttgtgctcgt gttctttgcg
    16501 gcggcgtgcg gcggcgcgac tgctgaatct ccactcacag tccagctcgg actcaaccac
    16561 gaccaaacga agcagatgct cgcgcagcat cagttctgcc tcgagacgtc gtcatcgacc
    16621 gtcgcggcga cccagcagaa gcaggtctat ccgcgctgca atcgcccggc ggcggagcac
    16681 ggtgaagcct gggtcgtcgc gatgtacgac ggtgacaagc tcgtcgagct gcgccgcttc
    16741 gagagctacg gcgacgacgc gcgcgcggtc gagcgctgga acgagctcat caccgagcgc
    16801 atgaagacat cgcagcccga cgatgcagcg ctccagcaga tcaaagagca gggcatgctc
    16861 gagccgggca cgcgctcggt caaagcattt cgcggcgagc ccggcaccgt cgtcggcgtc
    16921 tatttactca cgccgtcgcc gcccgcgaat gccaacgtgc tcgagaaggt cacgtatctg
    16981 aagtagcgag tccgaaaacg aaaacgcccg cgcgtgcggg cattcgttta cggaagcgag
    17041 tctgcgttac gggcgagcgc ctgcacacat cgcgtcgttc gcgccggtgc atgccgcgag
    17101 gcgagccgtc gccatgttcg tcgccgtgat gccggcgccc gtcgggttgc ccttctcgaa
    17161 ccagccattg gagaagttgc cgctgaccgg cgagccctgc tgcgtgtcga cgtccgtcgt
    17221 gttcgagaac cagttgttgt actggaagtc agcgttcatg ccgccgtaga acatgaggcc
    17281 gtacgacgac gtgctgatgt tcgagtgcgt gaccttgatg acgttgccgc ccgggttgaa
    17341 gtgcagctgg cagtgcgtcg tgtccgtctg gcccggctcc atgccgaccg tcgagtacga
    17401 cacttccatc gtgccgccgt tcatcatgag gaagtcgccg gctgcgtgat gcaggtgcga
    17461 gtcggcgatc gtcgccttgc ccgtgccgct cgtcgtgatg ttgccgcccg cttgatcgac
    17521 gaacttgaac ttcagctcgc cgcccgcgtt caccatgatg ccgttccagc ccatgccttc
    17581 cggcttgagc gtgactttgc tcgccgaggt gccctgcgca tcgaggatgc cgttgacctg
    17641 cagcgaggct tggcccttga gcgagaccgt cgtgcccgcc gcgatcgtca cggtgacgcc
    17701 cgggtcgatc gtcgtgatgc ccgagatgag gacgttgccg gtccagtcgg tcgacgccgt
    17761 gatgtgaccg ctgatgccgc ccgtggggcc ggagccgctg ccactgccgg agcccgagcc
    17821 cgttccatca tcgccgcctg tcccgtcatc tccggtcatg gttccgtcgc cgacgacgca
    17881 gccgatcagg ggcagggaca agagcagggc tagggtcttc ctcatggtgt gcaattctag
    17941 agcaatggat gtgccgcgag cgcatgcgga accgcccatg tgatcagcct gttacacggc
    18001 gtggatcgac cctcgatcgg gggtcgtcac cccacccggt ggggcatctc gtcatcgatc
    18061 cgcgctagct cgccggaaac aggaaccgca ggaaccgcca ggcgcgagct ttccaagcaa
    18121 gctcatcgtg ttgcgcgccg ggctcgagcc ggtagcagag cgcgttcgga ccggatgcgc
    18181 aggacggcga atcgccgcgc tgccaaccca ggtttacgag catgtcgcgc acctcggccg
    18241 tgtccgccgc gctgtccgcg ttcgacgcga gggcgccgcc gctgtcgagg tagatcgaga
    18301 ccggcatctt gctcgcgccg cgcagctgat cgcggaccgc cgtgccttcg tcttggccga
    18361 cccagaacga gctcgagagg cacgcgatgc cggcatacgt ctgcggatgc gcgagcgcga
    18421 gctccatcga gatcaggccg ccgagcgagc tgcccgcgac atacacgcgg ccatcggcgc
    18481 ggaccttcgc gacgaacttc ggctgcagct cgttgacctg gaagtcgatg aagttggccg
    18541 tcgcggtcgc atcgaggccg tactcgtcga ggcgcttgtt cgtcatgtcc gccgcgatca
    18601 cgacgactgg cgctacgcgc ttcgcggcga tctcggtatc gagcgtgacg ttcacttccc
    18661 agccggtgtg gccaaagcag cagtcgtggt cgtcccagac gttctggccg tcgtgcatga
    18721 acagcacggg atatttcgtc gacgcggtcg caggcgcatc gtagccagcc ggcagatacg
    18781 cggtgacgtc gcggcagttg ccgagcttgg cggagcacgc ttcgccgagg tacgcgagat
    18841 gaccgcgacc ggagtcgggc gtgttcagca ccgagttctt gccgtcggcg ttgcccgaga
    18901 aatcgtcata cgcgaatgcg ggattcagcg gatcgagtgt ccacgcctgc ccggtcacga
    18961 gcttgtacgt gtgaaagccg ctcgcgacat tgccgacgcc gagcaccacg gagctgccgc
    19021 aaaacgcctt cgtttcgagc gc
//
LOCUS       DATLBZ010000434         3351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_536809, whole genome shotgun sequence.
ACCESSION   DATLBZ010000434 DATLBZ010000000
VERSION     DATLBZ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536809"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VMZ53_22045"
     CDS             <1..1026
                     /locus_tag="VMZ53_22045"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31215.1"
                     /translation="STFAGNAGAAKERQEIVRYAINHVSETAGAANFTTGYGDGKYLL
                     LASDRRTDGVMLEALILEDPKNDLIPKIVTGLLAHRTKGHWLNTQENAFVLTALELYF
                     KTYEKITPNFVARVWLGNDYAGDHAFKGRTTEYHQIDIAMKDVATHDKSNLTIQKDGA
                     GRLYYRIGMKYAPEDLKLAPADYGFVVERTYEGVDDPKDVTQDKQGIWHIKAGKRVRV
                     KLSLVNENRRYHVALVDPMPAGLEALNPALATTGPIPTAQDPNDTTSRGRYWWWYGPW
                     YEHQNMRDERVEAFSSLLWEGVHSYEYVARATTPGHFVVPPPKAEEMYMPETFGRGAS
                     DRVYVEQ"
     gene            complement(1185..1448)
                     /locus_tag="VMZ53_22050"
     CDS             complement(1185..1448)
                     /locus_tag="VMZ53_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31216.1"
                     /translation="MSDPDLTLAILRDIRRKLDHGFAQSKADLAALNARMSVLEQRFD
                     VLSDTVNSFGDDHRTLIGYVKKQLVLAIKTLDLRVSKLERKSG"
     gene            1611..2132
                     /locus_tag="VMZ53_22055"
     CDS             1611..2132
                     /locus_tag="VMZ53_22055"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HUS31217.1"
                     /translation="MAVIFIDGNDLRANEVRQVIPGIELRVEQLQTGFATSAPEPEQK
                     ARDKVLAHPVTAACFAEAVDLTTMEGKSLRLELDSENENRFCKWWRETPAQMQLRIAI
                     RREGSAEIELIKATCQGRIADKPAGPLLLGWDRLFVPEGQDFTLAQLTDAGELTDFRR
                     AAYTDVARALGLH"
     gene            2236..3006
                     /locus_tag="VMZ53_22060"
     CDS             2236..3006
                     /locus_tag="VMZ53_22060"
                     /inference="COORDINATES: protein motif:HMM:NF042890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6748 domain-containing protein"
                     /protein_id="HUS31218.1"
                     /translation="MALALHIASNMIRASLFIAAVAAVIPACAASSGPADELGEETAA
                     DGEEGKGDAAGAFTFYNLIADNRACSLNSPSGCGIGFFLSRANRSTTQCGRGTPQSQC
                     KITELDWSGTALPPSVAKSYEDRLRDGQPLLVKGDVVPAANDAGLSFAVKELWVTNNP
                     EFVEGVFAVVKDNGIRCVRAPCPSLTERRLNSNLSAQITGLDFDASGAEQADIDLARE
                     QLYSADGLVVVGYRDYDSLGGKTRTVNRFFTKAPVPLH"
     gene            3052..>3351
                     /locus_tag="VMZ53_22065"
     CDS             3052..>3351
                     /locus_tag="VMZ53_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31219.1"
                     /translation="MGRFDDRASQPSRGPSDMFKWKVLRRSDEKPANFDELDKIRPPI
                     ADPAPAIAALASGAAVACWIGHASWTFRLGGKLCVIDPVWSQRLSGVVPRLVPPGV"
BASE COUNT          646 a         1157 c         1009 g          539 t
ORIGIN      
        1 tcgacgttcg cgggcaacgc aggtgcagcc aaagagcgcc aagagatcgt gcgctacgcg
       61 atcaaccacg tcagcgagac ggcgggcgcg gccaacttca cgaccggcta cggcgacggc
      121 aagtacctcc tcctagcgtc cgatcgccgg acagacggcg tgatgctcga ggcgctgatc
      181 ctcgaagacc ccaagaacga tctcattccg aagatcgtga cgggcctgct cgctcaccgc
      241 acgaaaggcc actggctcaa cacgcaagag aacgcgttcg tgctcaccgc gctcgagctc
      301 tacttcaaga cgtacgaaaa aatcacgccg aacttcgtgg cgcgcgtgtg gctcggcaac
      361 gactacgccg gtgaccacgc cttcaaaggc cggacgacgg agtaccacca gatcgacatc
      421 gcgatgaaag acgtcgcgac gcacgacaaa tcgaacctga cgatccaaaa ggacggcgca
      481 ggccgcctct actaccgcat cggcatgaag tacgcgcccg aagacctcaa gctcgcgcca
      541 gccgactacg gcttcgtcgt cgagcgcacg tacgaaggcg tcgacgatcc gaaagacgtc
      601 acgcaagaca agcaaggcat ctggcacatc aaggccggca agcgcgtgcg cgtgaagctc
      661 tcgctcgtca acgagaaccg tcgctatcac gtcgcgctcg tcgacccgat gcccgccggc
      721 ctcgaggccc tcaacccggc gctcgcgacg acaggcccga tcccaaccgc ccaagatccc
      781 aacgacacga cgtcgcgcgg tcgctattgg tggtggtacg gcccctggta cgagcaccag
      841 aacatgcgtg acgagcgcgt cgaagcattc tcgtcgctgc tctgggaagg cgtgcactcg
      901 tacgagtacg tcgctcgcgc gacgacgccc ggccacttcg tcgtcccacc gcccaaagcg
      961 gaggagatgt acatgcccga gacgttcggg cgtggcgcga gtgaccgcgt gtacgtcgag
     1021 cagtagctac tgcgacgcct tttcggctgc acgtttgagg cgcgcgtact gtctgctgag
     1081 tcggcgtatt accggatcgg cgatgagctc tctaagctcc tgccgagccg attcgaaaac
     1141 ggtcggtcgg gaaggcgcgg agcgctttcg acggcggcga atcatcagcc gctctttcgt
     1201 tcgagctttg agacgcgaag gtcgagcgtc ttgatcgcaa ggaccaactg tttcttcacg
     1261 tatccgatga gcgttcggtg atcgtcgccg aagctgttca cggtgtcgga aagaacatcg
     1321 aaccgctgtt cgaggacaga catccttgcg ttgagtgctg cgagatccgc tttgctttgt
     1381 gcgaagccgt gatcgagctt tctgcgaatg tcgcgaagaa tcgcgagcgt gagatcggga
     1441 tcggacacga ggcacctcca gtctaccgag cccactgagc aagccaaagg ccattcggaa
     1501 gtgcgcgttt cctcggtgcg atacgttgcg agttggccgc cgcgtggacg tcgtgtccgt
     1561 ctgccggcgg acgccggaca gcgcgccatc cgtcgtagta gggtcgccgc gtggcggtga
     1621 tcttcatcga cggcaacgac ctgcgcgcga acgaagtccg ccaagtcatc cccggcatcg
     1681 agctgcgcgt cgagcagctg cagaccggct tcgcgacgag cgcgcccgag cccgagcaaa
     1741 aagcgcggga caaagtgctc gcgcatcccg tcaccgccgc gtgcttcgcc gaagccgtcg
     1801 acctcacgac gatggaaggc aagtcgctgc gcctcgagct cgacagcgaa aacgagaatc
     1861 gcttctgcaa atggtggcgc gagacgccgg cacaaatgca gctgcgcatc gcgatccgcc
     1921 gcgaaggctc cgccgagatc gagctcatca aagcgacgtg ccaaggccgc atcgccgaca
     1981 agcccgccgg cccactcctc ctcggctggg atcgcctgtt cgttcccgaa ggccaagact
     2041 tcacgctcgc gcagctcacc gacgcaggcg agctcaccga cttccgccgt gccgcataca
     2101 ccgacgtcgc gcgcgccctc ggcctccact aacagcgttg ttacagcgcg agtactggct
     2161 tgtgctcgat cagccatggc aagtgagctt gccgcctcga ctctcgctag gtcgccgaga
     2221 tgtttgacgc ggcgcgtggc cctggcgttg cacatcgcga gcaacatgat tcgcgcatcc
     2281 ctgttcatcg ccgctgtcgc ggccgtcatt cccgcttgcg ccgcaagctc gggtcctgcc
     2341 gacgagctcg gcgaagaaac cgccgccgac ggtgaagaag gcaagggcga cgccgccggt
     2401 gccttcacgt tctacaacct gatcgccgac aaccgcgcat gcagcctcaa ttcgccgagc
     2461 ggctgcggca tcggcttctt cctgtctcgc gcaaaccgct cgacgactca atgcggccgc
     2521 ggcacgccac agagccagtg caagatcacc gagctcgact ggagtggtac cgcgctgcca
     2581 ccgtccgtcg caaagagcta cgaagatcgc ctccgcgacg gccagccgct gctcgtcaaa
     2641 ggcgacgtcg tgccggccgc caacgacgcc ggactctcgt tcgccgtcaa agagctctgg
     2701 gtcaccaaca atcccgaatt cgtcgagggc gtcttcgcag tcgtcaaaga caacggcatc
     2761 cgctgcgtcc gtgccccgtg cccgagcctg acggagcgcc gcctcaactc gaacctctcc
     2821 gcgcagatca ccggcctcga cttcgacgca tccggcgccg agcaagctga catcgacctc
     2881 gcgcgcgagc agctctacag cgcggacggc ctcgtcgtcg tcggctaccg cgactacgac
     2941 agcctcggtg gcaagactcg cacggtcaat cgcttcttca cgaaggcgcc agttccgctg
     3001 cactgaccaa cgcagcggtg tcgaccgcgg tagtggtagc gtggcaccac gatgggccgc
     3061 ttcgacgatc gcgcgtcaca acccagtcgc ggaccgagcg acatgttcaa gtggaaggtg
     3121 ctccgtcgca gcgacgagaa gcccgccaac ttcgacgagc tcgataagat ccgcccaccg
     3181 atcgctgacc cggcccccgc gatcgccgcg ctcgcctccg gcgctgccgt cgcatgctgg
     3241 atcggccacg catcatggac cttccgcctc ggcggcaagc tctgcgtgat cgatcccgtc
     3301 tggtcgcagc gcctctcggg cgtcgtcccg cgcctcgtcc caccgggcgt c
//
LOCUS       DATLBZ010000435        13272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1281000, whole genome shotgun sequence.
ACCESSION   DATLBZ010000435 DATLBZ010000000
VERSION     DATLBZ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281000"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VMZ53_22070"
     CDS             complement(<1..681)
                     /locus_tag="VMZ53_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HUS31220.1"
                     /translation="MEAKLDMVLEQVGRDKNIDKSVLKDALVQAILTAAKRTFGMNRE
                     MEAKFNDDTGTVDLFLIVKIVEEDDAIEGRELTVAQAKSAGLDAELDDELLFQIFYRA
                     EDEDKAREQDEKFGELIDLKNASKKFGRIAAQTAKQVIYQRVREAERDNVYNEYKDRK
                     GEIITGTVRRFERGALVVDVGKAEAVLPMREQVPRESYRVGDSIKAYVLDIDRNARGP
                     QIILSRTHK"
     gene            complement(693..1304)
                     /gene="rimP"
                     /locus_tag="VMZ53_22075"
     CDS             complement(693..1304)
                     /gene="rimP"
                     /locus_tag="VMZ53_22075"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HUS31221.1"
                     /translation="MSRNPLQQKLISIIEPVLEAAGFELVDLRFLLEQGGWVLRVQVD
                     TPLADVTDITQVPSDRVDLSDCENISRELSAVLDVDDPIPQAYHLEVSSPGIDRPLRT
                     AAHFAHFAGSEAKIQLGVPLQLEGGERKNFKGVLKGVADNKVSIDCDGTVFQLPIDDI
                     DHAKLVPDWDAVMHGQSGVGEKKPKPVKPGHRPSQKQKQKQQH"
     gene            1456..2286
                     /locus_tag="VMZ53_22080"
     CDS             1456..2286
                     /locus_tag="VMZ53_22080"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HUS31222.1"
                     /translation="MTWQLRVGAVQMCSTDDLQANLATCRRLVEQAVSEGAQLACLPE
                     CFSFLGRKEGDKLAIAETIEGSGPIMGLLRELAAKHGIHILGGGTPEVVTGDAKRTYN
                     TAVLVGPSGNVVSRYRKIHLFDVDIPGGAVLRESDATAPGSECVVVDIGGAKCGLSIC
                     YDVRFPELYRSLVKDRGAEVLFVPAAFTAHTGAAHWHLLLRARAIEDQAWVVAPAQWG
                     KHNEKRESYGHTLVVDPWGTIVAERETGDGVVMATLEGAAVEKRRTQMPCLSHAVLWK
                     "
     gene            complement(2287..2619)
                     /locus_tag="VMZ53_22085"
     CDS             complement(2287..2619)
                     /locus_tag="VMZ53_22085"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS31223.1"
                     /translation="MTDERRGSPRVAASIAGELEIDGGKSAIAITRDVGANGLLVFTR
                     LRECTGSVKLTVVYKDEMLVLTGHVVRMEPVEDSQLWRNKVAIAIDGDHSAITKLFSA
                     IAASDQTT"
     gene            complement(2640..3032)
                     /locus_tag="VMZ53_22090"
     CDS             complement(2640..3032)
                     /locus_tag="VMZ53_22090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31224.1"
                     /translation="MAEETEEPVPKLPRGRGLKLSGPELFRIFMTLIALVGVIVLARP
                     CASSVSNFVMGYEGSASSKLPKPGNVEPTTPTPSDLDQYEQLKPGMTEQELKAAIERA
                     KAKNAAATGSAAATGSAAASAGSAGSAQ"
     gene            complement(3049..3552)
                     /locus_tag="VMZ53_22095"
     CDS             complement(3049..3552)
                     /locus_tag="VMZ53_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31225.1"
                     /translation="MKSVLAFVVVGMVVSTAHADPFAVRNPRPDAPPSVTATQQPARI
                     EQRVEILDPGKSQRRTATIIVAGGGALYVSSLIVSLSMKSRYDDAIARLDSGEGDLQA
                     AVDDANHARRIAQVAGTGLFAAGAVAIGVGAYLYFTAPIKIRREHVAIVPSVDSQGVG
                     LSLSGGF"
     gene            complement(3588..4214)
                     /locus_tag="VMZ53_22100"
     CDS             complement(3588..4214)
                     /locus_tag="VMZ53_22100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31226.1"
                     /translation="MSDRRLANWTARDLETHIDELASAARRWRDGKGELPLVTLHLSS
                     GARHIGFVLERTRTTLAVQGVPQRGSYDLDVTVIPIARIEAVTLHNAQTLVEAAPPTP
                     EGATSMLDLRRRAKALADQLATRAGKPIAIEIGAGDVAELGPLFEHLRIALERVCADD
                     LGRASLAERVARIELRSGASGVSLASGTLVVAGPLAADRLQREIDAVL"
     gene            complement(4211..4606)
                     /locus_tag="VMZ53_22105"
     CDS             complement(4211..4606)
                     /locus_tag="VMZ53_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31227.1"
                     /translation="MGLAEKRWAADKKKTDEPAFVTQVASTLGFSVPVEIDWDTFSNN
                     LDDTQYLTHDSYGLPNLVKALSTITADDLGKEAIKGALKKIVIRPAPADDTAFTFADG
                     VITWKAYFGSSSSGYIYADAMQKTLEKAL"
     gene            4728..5900
                     /locus_tag="VMZ53_22110"
     CDS             4728..5900
                     /locus_tag="VMZ53_22110"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31228.1"
                     /translation="MRIAVLLLIAGCRWRFDTTGDDLPTADGGFVVDDAGQFVHCDVD
                     TDCGRCQRCENSFCQRATFADVDLGQYNGCALDERGQVWCWGLNDYQAVAPELTGGVV
                     VPRPRRRTDITATIDRIALGWGVMYLRTTSHELMSRGSLDGDVDDTRTFWDGLSASHF
                     NGCATTTTGELYCSGLDDDGQLGRGTLTTAPRPLGLFGTDTDWARSSTFNATCAIKTN
                     GTLWCVGRNDFAQLGRGTTTPRETDIAQLGTANDWSEIALDEHTTCGIRGGQLWCWGL
                     EFGTNATQPSPLQVGTATGWSTLDAYYRCAVAMLGGTPYWACSDALALATSLQAPTTF
                     GWTEMPVTPDPGTRIVLGATAGCFRQNGQWMCWGDNRNGAVGVDDLSVVAPTPLCE"
     gene            complement(5906..7636)
                     /gene="recJ"
                     /locus_tag="VMZ53_22115"
     CDS             complement(5906..7636)
                     /gene="recJ"
                     /locus_tag="VMZ53_22115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HUS31229.1"
                     /translation="MQVRVRSVDERLVDRIAGALRVRPATARCLVARGVVEPQDAQCF
                     IDPRLAALRPPAGLAGLALAVGRIADAVVKGERIGVFGDYDVDGITTAALLTSFLRAA
                     GAHVECAVARRDAGYGFTASAASDFVARGCKVIITGDCGTSDIESIRVANAAGVEVIV
                     VDHHTVPSADVVHPAFSLVNPFRADSTFPFRGMASVGLGFYVAASVRTELRDRGHFRS
                     RPEPDVRDLLDLVALGTIADLVPLAAENRILTALGLRRLQSRARPGIAALLESAGVDI
                     DREIDANTIAWKIAPRINAPGRLGAAEPSLSLLLAEGESATERARVLEEANTQRRSVQ
                     DKVMREALAMLGDADPGPAIVLAGEGWAPGVVGIVAAKLVDKYQRPAFVVGIDPATGL
                     GRGSARTSAGVNLYEALASASRELGPRLDRFGGHAAAAGFTVQRENVAALAEALQSAV
                     ATRFAEGSGPIARSRDVDAEVKLAEVDERLAQELAGLGPFGQENPAPMLVTRGARVTA
                     VRRVGDGSHLKLTLEDNTQTTRSAIGFKLGDRPVDVGAHLDLAFVPTVSTWQGRRSAE
                     LEIADLAILS"
     gene            complement(7700..8470)
                     /locus_tag="VMZ53_22120"
     CDS             complement(7700..8470)
                     /locus_tag="VMZ53_22120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS31230.1"
                     /translation="MDADTARRMWNDSADAYNHFVESGLDYYRTDLHGPALLDACGDV
                     RGLRVLDLGCGQGWFSRQLAEAGAQVTGIDWSAAMIEHARRLGPPEIHYEVMDAADVA
                     TAFAAGSFDLATGCMSIMDMPKPGAVLAAARQVAPRIVFSIPNPVTDAPHRVWKRDAQ
                     GGKLALEIDRYFESTTTIMRWNMKRLVHHFETVQYRYTLEQWSRMLEDAGLVIARLRE
                     PRPSDAAVAARPELADAARLPYFLIVDARRSFSDPGII"
     gene            8608..8781
                     /locus_tag="VMZ53_22125"
     CDS             8608..8781
                     /locus_tag="VMZ53_22125"
                     /inference="COORDINATES: protein motif:HMM:NF015035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005186 - pheromone activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="HUS31231.1"
                     /translation="MTNNKTETTFETIDTANLENVTGGYMSENRLSATPESGGKLISG
                     AIDRQERETNMPH"
     gene            complement(8809..10095)
                     /gene="secF"
                     /locus_tag="VMZ53_22130"
     CDS             complement(8809..10095)
                     /gene="secF"
                     /locus_tag="VMZ53_22130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HUS31232.1"
                     /translation="MDQHKHFRYLIPPGNNFAFVRKFKYWLIASVVLMAAAVAALFIN
                     KSTRGEYMNWTIDFKGGTEIIFAFKDTNTNQFVKVDPAKVRKALEDAGDHGVELSDIA
                     YTEEINGKEVPVSGMLIRSPRFSALKDQQRVDTSKGFIEKFADRKVGKATWSGDRLFV
                     RTSKPVTHEEAAAFFAAHGLELKPWSQKEIDQYSQPDEGTGELNAVYSIWGLDRQYGQ
                     TLEKAIGNVKAITVNSYGVGAKAGEKLRDDAAKSIIYAIFLIMLYLAFRFDIRYAPGA
                     AFATIHDAVMVIGVFALTWTEVSLTTVAGLLTVIGYSVNDTVIVFDRIRENQAKHKDK
                     KLERIVDLSLNEMLVRTILTSSTVFATTLIMNIFGTGLVKNFAFAMNVGVIVGTYSSI
                     FLATPIFLWVSRTWYSGPPKARSIPAAVPKDGAVEA"
     gene            complement(10106..12121)
                     /gene="secD"
                     /locus_tag="VMZ53_22135"
     CDS             complement(10106..12121)
                     /gene="secD"
                     /locus_tag="VMZ53_22135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HUS31233.1"
                     /translation="MDRSLKWRTAILAVGTIFFILILLPSFIPKQSLPKLPWPANKLW
                     SSKMSLGLDLQGGLHLVYSIDLDKAIDDRASEIKRSMDSRFTDEKVKGAVKTPAAPVG
                     AVTVLIDNAPDVPKVKKWIEEDHGKDITYIECSKAEPPNTVCFRVSSTFGDSLKKDAL
                     SNAVSTIRERINEKGVAEPNVVEKGDQIIVELPGLDDEQIQETKDIIARTAKLEMKVV
                     DDCTVYSPTGCTASNSTHDGSPYMKKLFSKVGSDTKGNPTDPDAAKLEIRAEVDQWRP
                     DEGGAVHTDYYLIAPDREETVPLAWAKKHGCINKESVVENGTVRCNITGRQIIERYVY
                     GDPELGMQGLIAQDPSFKAPDDRQLGFEPLDVQPNAKDKRHFWRTYYLERAVRLTGSA
                     ISNAMGSYDPNTNRPVVLLDFNRFGGRIFGDVTAQIVGMKFATILDDKVKSAPIINGA
                     IRGGRASITMGGGNVDVQEREREELVAVLKTGSLPAPLREESSARVGPSLGMDAIAKT
                     RLSFILGIALVILIMVGIYRWSGMIAVFAVVFHIVVTLAVMALFGATLTLPGIAAIVL
                     SIGMEVDGNILIYERIREELLQGKSVRGAIDLGFSRAFSAILDGQLTTAAAGWVLLNY
                     GSGPIKGFAVMLLVGVFTTVTTNIWVTRILFDWVVTRKKGQLQSLSI"
     gene            complement(12170..12469)
                     /gene="yajC"
                     /locus_tag="VMZ53_22140"
     CDS             complement(12170..12469)
                     /gene="yajC"
                     /locus_tag="VMZ53_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HUS31234.1"
                     /translation="MQALSGMFPILMIFGIFYFVLLRPQMRRQKETQAMLSKLGKGDE
                     VITRGGLIGKITGVSDDGIVVLELQEKVRVRIPRSFIEGKWEGKVVEEAGAKKAA"
     gene            complement(12523..>13272)
                     /locus_tag="VMZ53_22145"
     CDS             complement(12523..>13272)
                     /locus_tag="VMZ53_22145"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HUS31235.1"
                     /translation="DDGVTFRSHIDGSKHRLTPEVSMHIQKVLGSDIAMCFDQCPPGD
                     APADVQEVALARTTRWAARCKAAPHAEGQAVFGIVQGGIDVPRRMRHMAEITALDFDG
                     HALGGLAVGESVADTYRILDEVAHQLPAEKPRYLMGVGTPADLEKGIAAGIDMFDCVM
                     PTRNARNGYLFTSGPRVNIPNAANRLDLGPIDPECPCSTCRTHSRAYLRHLYLAKEIL
                     YARLATLHNLTFYARHVRRLRDRILADGMRA"
BASE COUNT         2310 a         4310 c         4281 g         2371 t
ORIGIN      
        1 cttgtgcgtg cggctgagga tgatctgcgg gccacgcgcg ttgcgatcga tatcgaggac
       61 gtacgccttg atcgaatcac cgacgcgata gctctcgcgc ggcacttgct cgcgcatcgg
      121 caggacggct tccgccttgc ccacgtcgac gacaagcgcg ccacgctcga agcggcgcac
      181 ggtgcccgtg atgatctcgc ccttgcggtc tttgtactcg ttatagacgt tgtcgcgctc
      241 ggcctcgcgg acgcgctggt agatgacctg cttggcggtc tgcgcggcga tgcggccgaa
      301 cttcttgctc gcgttcttga ggtcgatcag ctcgccgaac ttctcgtcct gctcgcgtgc
      361 cttgtcctcg tcctcggcgc gatagaaaat ctggaacagc agctcgtcat cgagctcggc
      421 atcgagaccg gcgctcttgg cttgcgcgac cgtgagctcg cggccttcga tggcgtcgtc
      481 ctcttcgacg atcttcacga tgaggaacag atcgaccgtt cccgtgtcgt cgttgaactt
      541 cgcttccatc tcgcggttca tgccgaacgt acgtttggcg gccgtaagaa tcgcctgcac
      601 gagagcgtcc ttgagaacgc tcttgtcgat gttcttatct cggccgacct gctcgagaac
      661 catgtcgagt ttggcttcca tgaccatttc cctcagtgct gctgcttctg cttttgcttt
      721 tgactcggac gatgtccggg cttcacaggc ttgggtttct tttcgccgac gccagactgg
      781 ccgtgcatga cggcgtccca gtcgggcacg agcttggcgt gatcgatgtc gtcgatcggg
      841 agctggaaca ccgttccgtc gcagtcgatc gagactttgt tgtccgcgac gcccttgagg
      901 acgcccttga agttcttgcg ctcgccgccc tcgagctgca gcggcacgcc gagctggatc
      961 ttggcttcgc tgccggcaaa gtgcgcgaag tgcgccgccg tgcgcagcgg gcgatcgatg
     1021 ccaggcgagc tgacctcgag gtgatacgcc tgcgggatcg ggtcgtcgac gtcgaggaca
     1081 gccgacagct cgcgcgagat gttctcgcag tcactcagat cgacgcggtc actcggcacc
     1141 tgcgtgatgt ccgtgacgtc ggcgagcgga gtgtcgacct ggacgcgaag cacccagccg
     1201 ccttgctcca atagaaagcg cagatcgacg agctcgaagc cggccgcttc gaggaccggc
     1261 tcgatgatcg aaatgagctt ttgttggagt ggattgcggg acatgtctga acgagggcga
     1321 aaaaaaaagg tggccggacg gccaccccta ccgcgaagcg atatgaagct gcgggatggg
     1381 taccaaaccc ctgatcagac cgcaagtgct tttgaggctc ctacgcggtc gccgtaggcc
     1441 aggtacagtc ccgaaatgac atggcagctg cgcgtcggtg ccgtccagat gtgctcgacg
     1501 gacgatctcc aagcgaacct tgcaacgtgc cgccggctcg tcgagcaagc ggtctcggag
     1561 ggtgcccagc tcgcctgcct gcccgagtgc ttctcgttcc tcggccgcaa ggaaggcgac
     1621 aagctcgcga tcgccgagac gatcgaaggc tcgggtccca tcatgggcct gctccgcgag
     1681 ctcgccgcga agcacggcat ccatatcctc ggtggcggca ctccagaggt cgtcaccggc
     1741 gacgcgaagc gcacgtacaa caccgccgtc ctcgtgggac cgagcggcaa cgtcgtatcc
     1801 cgctatcgca agatccatct gttcgacgtc gacatcccgg gcggtgcagt tctccgcgaa
     1861 tcggatgcga ccgcgcccgg cagcgagtgc gtcgtcgtcg acatcggcgg cgcgaagtgc
     1921 gggctatcga tctgctacga cgtgcgattc cccgagctct atcgctcgct cgtcaaagac
     1981 cgcggcgcag aggtgctctt cgtccctgcc gcgttcaccg cgcacaccgg cgcggcgcac
     2041 tggcatctgc tgctacgtgc gcgcgcgatc gaagaccagg cgtgggtcgt cgcgccggcg
     2101 cagtggggca agcacaacga gaagcgcgag agctacggtc acacgctcgt cgtcgatccg
     2161 tggggcacga tcgtggccga acgcgagacg ggtgacggcg tcgtgatggc gacgctcgag
     2221 ggcgcggccg tcgagaagcg acgcacgcag atgccgtgcc tctcccacgc cgtgctctgg
     2281 aaatagctac gtcgtctggt cggacgcggc gatcgcgctg aacagcttcg tgatcgcgct
     2341 gtgatcgccg tcgatcgcga tcgcgacctt gttgcgccac agctgcgagt cttcgacggg
     2401 ctccatgcgg acgacgtgtc cggtcagcac gagcatttcg tccttgtaga cgaccgtcag
     2461 cttcacgctg cccgtgcatt cgcgcaagcg cgtgaacacg agcaggccat tcgcgccgac
     2521 gtcgcgcgtg atcgcgattg ccgacttgcc accgtcgatc tccagctcac ccgcgatgct
     2581 tgccgcaact cgcggtgatc cacgccgttc gtccgtcacg tccccaccct accagcgcgt
     2641 cactgcgcac tgccagcaga acccgccgag gcggcggcgc tgcccgtcgc tgcagcgctg
     2701 cccgtcgctg cggcgttctt cgccttcgcg cgctcgatcg cggccttcag ctcctgctcc
     2761 gtcatgccgg gcttcagctg ttcgtactgg tcgagatccg acggagtcgg cgtcgttggc
     2821 tctacattgc ccggcttcgg cagcttcgat gaagcgctgc cctcgtagcc catcacgaag
     2881 ttcgagaccg agctcgcgca gggacgcgcc agcacgatta caccgacgag cgcgatcagc
     2941 gtcatgaaga tccgaaacag ctcgggcccc gagagcttga gaccgcggcc tcgcggcagc
     3001 ttcggtacgg gctcttcggt ttcttcggcc acgatcgaag tctgacgctt agaagccgcc
     3061 gctcaaggac aaaccgacgc cttggctatc caccgacgga acaatcgcta cgtgctcgcg
     3121 gcggatcttg atcggcgcgg tgaaatacag gtacgcaccg acgccgatcg cgacagcgcc
     3181 cgcggcgaac agcccggtgc cggcaacctg cgcgatccgg cgcgcatggt ttgcgtcgtc
     3241 gacggcagct tgcagatcgc cttcgcccga gtcgaggcgc gcgatcgcat cgtcgtagcg
     3301 cgacttcatc gagaggctga caatgagcga cgacacgtag agtgcaccgc cgccggcgac
     3361 gatgatcgtg gccgtgcgcc gttgcgactt acctggatcg agaatctcga cgcgttgctc
     3421 gatccgcgcg ggctgctgcg tggcggtcac gctcggcggc gcgtccggtc gcggattacg
     3481 aacggcgaac ggatctgcat gagcggttga gaccaccatc ccgacgacca cgaatgcgag
     3541 aacgctcttc atgagcgggt tgtatcaccg acgcatgacg agcgcgctca caacacagcg
     3601 tcgatctcgc gctgcagcct gtcggcggcg agaggtccgg ccaccacgag cgtgccgctc
     3661 gcgagggaga caccagatgc gccactgcga agctcgatcc gcgccactcg ctcggcgagc
     3721 gatgcccgcc cgaggtcatc ggcacagacg cgctcgagcg cgatgcgcag gtgctcgaac
     3781 agaggcccga gctcggcgac gtcgccggcg cctatctcga tcgcgatcgg ctttcccgcg
     3841 cgcgtcgcga gctgatcggc gagcgccttc gcgcgacgac gcaagtcgag catcgacgtc
     3901 gcgccttccg gcgttggcgg cgctgcttcg acgagcgtct gcgcgttgtg cagcgtgacc
     3961 gcttcgatgc gcgcgatcgg gatgaccgtc acgtcgagat cgtacgagcc acgctgcgga
     4021 acgccttgca ctgcgagcgt cgtgcgcgtg cgttcgagaa cgaagccgat gtgacgcgca
     4081 cccgatgaga gatgcagcgt cacgagcgga agctcaccct tgccgtcgcg ccatcgccgc
     4141 gctgctgatg cgagctcgtc gatgtgcgtc tctagatcgc gcgccgtcca attcgcgaga
     4201 cggcgatcgc tcaaagcgct ttctcgagcg tcttctgcat cgcgtcggcg tagatgtagc
     4261 cactgctcga gctaccgaag tacgccttcc aggtaatcac gccatccgcg aacgtgaacg
     4321 cggtgtcgtc ggcaggcgcc ggacgaatga cgatcttctt gagcgcgccc ttgatcgcct
     4381 ccttgccgag atcgtctgcg gtgatcgtcg acaacgcctt cacgagattc ggaaggccgt
     4441 agctgtcgtg cgtcagatac tgcgtgtcgt cgaggttgtt gctgaaggtg tcccagtcga
     4501 tctcgaccgg aacgctgaac cccagcgtcg acgcaacttg tgtgacgaac gcaggttcgt
     4561 cggtcttctt cttgtcggct gcccaacgtt tctctgccaa gcccatgaag gctcctttcg
     4621 atgaattgga acgcagccag tctcccgtag attccagcaa aagtgtcacc cgcgtgttca
     4681 ctcccggcga cgcacggcgt cagcgacgtg ggatacgatg caagctcatg cgcatcgcgg
     4741 tgttgttgct gatcgccggc tgtcgctggc ggttcgacac caccggcgat gacctgccga
     4801 ctgccgacgg cgggttcgtc gtcgatgacg cggggcagtt cgttcattgc gacgtcgaca
     4861 ccgactgcgg ccgatgccag cgctgcgaga attcattctg ccaacgcgcg acgttcgccg
     4921 atgtggacct cggccagtac aacggctgcg ccctcgatga gcgaggccaa gtctggtgct
     4981 gggggctcaa cgattaccaa gcggtggcgc ccgagctaac aggtggcgtc gtcgtcccgc
     5041 gcccgcgccg ccgcaccgac atcacggcga cgatcgatcg catcgctctc gggtggggcg
     5101 tcatgtacct gcggacgacg tcgcacgagc ttatgagcag aggctctctc gatggcgatg
     5161 tcgatgacac gcgcacgttt tgggacgggc tcagcgcgtc gcacttcaac ggctgcgcca
     5221 cgactacgac gggcgagctg tattgctcgg gcctcgacga cgatggccaa ctgggtcgcg
     5281 gcacgctgac gaccgcacca cgaccacttg gcctgttcgg cacggacaca gactgggcgc
     5341 gctcgtcgac gttcaacgcg acgtgtgcga tcaagacaaa cggcacgctg tggtgcgtcg
     5401 gcaggaacga cttcgcccag ctcggccggg ggacgaccac gccgcgcgaa acggacatcg
     5461 cacagctcgg gaccgcgaac gactggagcg agatcgccct ggacgagcac acgacctgcg
     5521 gcattcgtgg cggacagctc tggtgctggg gtctcgagtt cggcaccaac gcgacgcaac
     5581 cgagcccact gcaggtaggc acggcaacgg ggtggtcgac gctcgatgcg tactatcgct
     5641 gcgcggtcgc gatgctcggc gggacgccgt actgggcgtg cagcgacgcg cttgcacttg
     5701 caacgtcctt gcaggcgccg acgacgttcg gatggacgga gatgccggtc acgcccgatc
     5761 cgggcacgcg tatcgtgctc ggcgcaaccg ccggctgttt tcggcagaac ggccagtgga
     5821 tgtgctgggg agacaatcgc aacggtgccg tcggcgtcga cgatctgtcg gtcgtcgccc
     5881 cgacaccgct ctgcgagtag cgcgactacg acaggatcgc gaggtccgcg atttcgagct
     5941 ccgcgctgcg cctgccctgc cacgtcgaga cggtgggcac gaatgcgaga tcgagatgcg
     6001 cgccgacgtc gaccggacga tctccgagct tgaacccgat cgcactgcga gtggtctgcg
     6061 tgttgtcctc gagcgtgagc ttgaggtgcg agccatcgcc gacccggcgc acggcggtga
     6121 cgcgcgcgcc gcgcgtgacg agcatcggcg ctgggttctc ctggccgaac gggccgaggc
     6181 ctgcgagctc ttgcgcgagg cgttcgtcga cctctgcgag cttcacttcc gcatcgacat
     6241 cgcggctgcg cgcgatcggg cccgagcctt ccgcgaatcg cgtcgcgacg gcggactgca
     6301 acgcttcggc gagtgccgcg acgttctcgc gctgcacggt gaatccagca gcggccgcat
     6361 gaccgccaaa gcgatcgagg cgcgggccga gctcgcgtga tgcgctcgcg agtgcttcgt
     6421 agaggttgac gcctgcggag gtgcgcgccg agccgcgacc gaggcccgtg gccggatcga
     6481 tcccgacgac gaacgcgggc cgctgatact tgtcgacgag cttcgccgcg acgatgccga
     6541 cgacgccggg cgcccagcct tcgcctgcca gcacgatcgc aggccccgga tcggcgtcgc
     6601 ccagcatcgc gagcgcctcc cgcatgactt tgtcctgcac ggagcggcgc tgcgtgttcg
     6661 cctcttcgag gacgcgcgcc cgctcggtcg cggactcgcc ttcggcgagc agcagcgaca
     6721 gcgaaggctc ggcggcaccg aggcgtcccg gggcgttgat gcgcggcgcg atcttccagg
     6781 cgatcgtgtt cgcgtcgatc tctctgtcga tatcgacacc cgccgattcg agcagagctg
     6841 cgatgcccgg ccgcgccctg ctctgcagcc ggcgaagccc gagcgccgtg aggatccgat
     6901 tctcggcggc aagcggtacc aggtccgcga tcgtgccgag cgcgacgagg tccagcagat
     6961 cgcgaacatc gggctccggc cgcgagcgga aatgaccgcg atcgcgaagc tccgtgcgaa
     7021 cgcttgccgc gacgtagaag ccgaggccca ccgaggccat gccgcggaac ggaaacgtgg
     7081 agtcggcacg gaagggattc acgagcgaga acgcgggatg cacgacatcc gccgacggca
     7141 ccgtgtggtg atcgacgacg atcacctcga cacccgcggc attcgcgacg cgaatcgact
     7201 cgatgtcgct cgtgccgcag tcaccggtga tgatgacctt gcaaccacgc gccacaaaat
     7261 cactcgccgc gctcgccgta aacccgtagc cagcatcgcg acgcgcgacc gcgcactcga
     7321 cgtgcgcacc cgcagcgcgc agaaacgacg tcagcagcgc ggcggtcgta atcccatcga
     7381 cgtcgtaatc gccgaacacg ccgatgcgct cgcctttgac gaccgcgtcc gcaatacgac
     7441 cgactgcgag cgccagaccc gcgaggcccg cgggcggccg cagcgccgca agtcgcggat
     7501 cgatgaagca ctgcgcatcc tgaggctcga cgaccccgcg cgcgacgaga cagcgagcgg
     7561 ttgccgggcg cacacgaagc gcgccggcga tgcgatcgac gagacgttcg tcgactgagc
     7621 ggacgcgtac ctgcacgcga gcacgctacg cagtcggtct gacatcgaaa cgtcctgatc
     7681 tcgttgagat ccctgtggat tagatgatcc cgggatcgct gaacgatcgt cgagcgtcga
     7741 cgatcaagaa gtacggcagg cgcgcagcgt cggcgagctc gggtcgcgca gcaaccgcag
     7801 catcgctcgg acgcggttcg cgcaatcgcg cgatcacgag gcccgcatct tcgagcatgc
     7861 ggctccactg ctcgagcgtg tagcgatact gcacggtctc gaagtgatgc acgaggcgct
     7921 tcatgttcca gcgcatgatc gtcgtcgtcg attcgaagta gcgatcgatc tcgagcgcga
     7981 gcttgccgcc ctgcgcgtcg cgcttccaca cgcgatgtgg cgcgtcggtc accgggttcg
     8041 gaatcgagaa cacgatgcgc ggcgcgacct gcctcgcggc tgccagcacc gcaccaggtt
     8101 tcggcatgtc catgatcgac atgcagcccg tcgcgagatc gaacgagccg gcggcaaacg
     8161 ccgtcgcgac gtccgccgca tccatgacct cgtagtggat ctccggcggg cccaagcggc
     8221 gtgcatgctc gatcatggcc gccgaccaat cgatgcccgt cacctgcgcg cccgcctctg
     8281 cgagctggcg actgaaccag ccctggccgc aaccgagatc cagtactcgc aatccgcgaa
     8341 cgtcgccgca cgcgtcgagc agcgccggcc catgcagatc cgtacggtaa tagtcgagcc
     8401 cgctctcgac gaagtggttg taggcgtcgg cagagtcgtt ccacatgcgg cgggccgtgt
     8461 cggcgtccac tcgctcagtc tacaagcgat gcccacgccg tagccggcgg gctacgagct
     8521 gcagttcgca gacgacatcg aatgtcagtc tgcccgattc gtttcaacaa catatgtacg
     8581 gcatgtccgc tgcactacag acggaacatg acgaacaaca agaccgagac gaccttcgag
     8641 acgatcgaca ccgccaacct cgagaacgtg acgggcggct acatgtccga gaaccggttg
     8701 tccgcgacgc ccgaatcagg cggcaagctg atcagcggcg cgatcgatcg gcaagagcgc
     8761 gaaacgaaca tgccgcactg agcgacaggc gcagcggcgc gagtcggctc aggcttcgac
     8821 ggcgccgtct ttggggacag ctgccggaat gcttcgggcc ttcggcgggc ccgagtacca
     8881 ggtccgcgag acccacagga agatcggcgt cgcgaggaag atcgacgagt acgtaccgac
     8941 gatcacgccg acgttcatcg caaacgcgaa gttcttcaca aggcccgtgc cgaagatgtt
     9001 catgatcagc gtggtcgcga acaccgtcga cgacgtgagg atcgtgcgca cgagcatctc
     9061 gttgagcgag aggtcgacga tgcgctcgag cttcttgtcc ttgtgcttcg cctgattctc
     9121 gcgaattcgg tcgaagacga tcacggtgtc gttgacggag taacctatga ccgtcaacag
     9181 gcctgcgacc gtcgtcagcg agacctccgt ccacgtcaat gcgaacacgc cgatgaccat
     9241 gaccgcgtcg tggatcgtcg cgaacgcggc accgggcgcg taccggatat cgaaccggaa
     9301 cgcgaggtac agcatgatga gaaagatggc gtagatgatc gacttcgccg cgtcatcgcg
     9361 cagcttctcg ccggccttcg cgccaacgcc gtacgagttg accgtgatcg ctttgacgtt
     9421 gccgatcgcc ttctcgaggg tctgaccgta ctggcggtcg aggccccaga tcgaatagac
     9481 ggcgttgagc tcgccggtgc cttcatcggg ctgcgagtac tgatcgattt ccttctgcga
     9541 ccacggcttc agctcgaggc cgtgcgcggc gaagaacgca gccgcttcct cgtgcgtgac
     9601 cggcttcgac gtgcgcacga agagccgatc gccggaccac gtcgccttgc cgaccttgcg
     9661 gtccgcgaac ttctcgatga agcccttcga cgtgtcgaca cgctgctggt ccttgagagc
     9721 ggagaaccgc gggctgcgga tcagcatgcc gctaaccggg acttccttgc cgttgatctc
     9781 ctcggtgtac gcgatgtccg agagctcgac gccgtgatcg ccggcgtctt cgagagcctt
     9841 gcgcaccttc gccggatcga ccttcacgaa ctggttcgtg ttggtgtcct tgaaggcgaa
     9901 gatgatttca gtgccgcctt tgaaatcgat cgtccagttc atgtactcgc cgcgcgtgct
     9961 cttgttgatg aagagcgctg cgacggcagc agccatcagg acgacggacg cgatgagcca
    10021 gtacttgaac ttgcgaacga acgcgaagtt gttgcccggc gggatcaggt agcgaaagtg
    10081 cttgtgttgg tccatggccg ggtcctcaga tcgaaaggga ctgcaattgg cccttcttcc
    10141 tggtgacaac ccagtcgaag aggatgcgcg tgacccaaat gttggtggtg accgtggtga
    10201 agacgccgac gagcagcatc acggcgaagc ccttaatcgg accggagccg tagttcagga
    10261 gcacccagcc agcagcggcg gtcgtgagct gaccgtcgag gatggcggag aatgcgcgcg
    10321 agaacccgag gtcgatggcg cctcgcaccg acttgccttg taacagctct tcgcgaattc
    10381 gttcgtagat gaggatgttg ccgtcgacct ccatgccgat cgacagcacg atcgcggcga
    10441 tgccgggcaa cgtcagtgtc gcgccgaaca gcgccatgac cgccagcgtg accacgatgt
    10501 ggaacacgac ggcgaacacc gcgatcatgc ctgaccagcg atagatgccg accatgatga
    10561 ggatgacgag cgcgatgccg aggatgaacg acagccgcgt cttcgcgatc gcgtccatac
    10621 cgagcgatgg gccgaccctc gcagagctct cctcgcgaag cggtgccggc agcgagccgg
    10681 tcttgagcac ggcgacgagc tcctcgcgct cgcgctcctg aacgtcgacg ttgccgccac
    10741 ccatcgtgat cgatgcgcgg ccaccgcgaa tcgcgccgtt gatgatcggc gccgacttca
    10801 ccttgtcgtc gaggatcgtc gcgaacttca tgccgacgat ctgcgcggtc acatcaccga
    10861 agatgcggcc gccgaaccgg ttgaagtcga ggagcacgac cgggcggttc gtgttcggat
    10921 cgtagctgcc catcgcgttc gagatcgcag agccggtcag gcgaactgcg cgctcgaggt
    10981 agtacgtgcg ccagaagtgg cgcttgtcct tcgcgttcgg ctgaacgtcg agaggctcga
    11041 agccgagctg gcgatcgtcg ggagccttga acgacgggtc ctgcgcgatg aggccctgca
    11101 tcccgagctc gggatcgccg tatacgtagc gctcgatgat ctggcgcccc gtgatgttgc
    11161 agcgaacggt gccgttctcg acgaccgatt ccttgttgat gcagccgtgc ttcttcgccc
    11221 acgcgagcgg cacggtctct tcgcgatccg gcgcgatcag gtagtagtcc gtgtgaactg
    11281 cgccgccctc gtcggggcgc cactggtcga cttccgcgcg aatctcgagc ttggccgcgt
    11341 cgggatccgt cgggttgccc ttcgtgtcgc tgccgacctt cgagaacaat ttcttcatgt
    11401 acggcgagcc gtcgtgcgtc gagttcgacg cggtgcagcc cgtcgggctg tacacggtgc
    11461 agtcgtcgac gactttcatc tcgagcttcg cggtgcgcgc gatgatgtcc ttcgtctctt
    11521 ggatctgctc gtcgtcgaga ccggggagct cgacgatgat ctggtcgccc ttctcgacga
    11581 cgtttggctc ggcgacaccc ttctcgttga tgcgctcacg gatcgtcgac acggcgttcg
    11641 acagcgcgtc cttcttgagg ctgtcgccga acgtcgacga cacgcggaag cacacggtgt
    11701 tcggcggctc cgccttcgag cactcgatgt acgtgatgtc cttgccgtgg tcctcttcga
    11761 tccacttctt gaccttcggc acatccggcg cgttgtcgat cagcaccgtc acggcgccga
    11821 ccggcgcggc cggcgtcttc acggcgccct tcaccttctc atcggtgaag cgcgagtcca
    11881 tcgatcgctt gatctccgac gcgcggtcgt cgatcgcctt atcgagatcg atcgaataga
    11941 cgagatgcag gccgccttgg aggtcgaggc ccaagctcat cttgctcgac cacagcttgt
    12001 tcgccggcca tggcagcttc ggcaggctct gcttcgggat gaagctcggc agcagaatca
    12061 gaatgaagaa gatcgtgccg acggcgagga tggccgttcg ccacttgaga gagcgatcca
    12121 tgaagcacct atcagttcgg gcgcgaactc actaacggag acgagataat cacgcagcct
    12181 tcttggcgcc agcttcctcg acgactttcc cttcccactt accctcgatg aagctgcgcg
    12241 gaatacgcac gcgaaccttc tcttgcagct cgaggacgac gatgccgtcg tccgaaacgc
    12301 ccgtgatctt gccgatcagg ccgccacgcg tgatgacttc gtcacccttg ccgagcttgg
    12361 acagcatcgc ttgggtctcc ttctgtcgac gcatctgcgg gcgcagaagc acgaagtaga
    12421 agatgccgaa gatcatcaga atcggaaaca tgccggacag tgcttgcatg gcctctaacc
    12481 tgttaaaaac ccgggggttc gtagcaccgg gccacgacag ggtcaagcac gcatgccgtc
    12541 ggcgaggatc cggtcgcgca gacgccgcac gtgcctcgcg tagaacgtga ggttgtgcag
    12601 ggttgcgagg cgcgcgtaca ggatctcttt ggccaggtaa agatgtcgca ggtaggcccg
    12661 cgagtgcgtg cggcacgtcg agcaggggca ctcggggtcg atcgggccga ggtcgaggcg
    12721 gttcgccgcg ttcgggatgt tcacgcgcgg gcctgacgtg aacaggtagc cgttgcgcgc
    12781 gttgcgagtc ggcatcacgc aatcgaacat gtcgatgccg gccgcgatgc ccttctcgag
    12841 gtccgccggc gtgccgacgc ccatcaagta gcgcggcttc tcggccggca gctggtgcgc
    12901 gacctcgtcg aggatccggt aggtgtccgc gacgctctcg ccgacggcaa gtccgccgag
    12961 cgcgtgacca tcgaaatcga gcgccgtgat ctctgccatg tgacgcatgc ggcgtgggac
    13021 atcgatgccg ccttgcacga tgccgaacac cgcctgccct tctgcatgcg gcgcggcctt
    13081 gcagcgcgcg gcccatcgcg tcgtgcgagc gagcgcgacc tcttgcacgt ccgcgggagc
    13141 atcaccgggc ggacattgat cgaagcacat cgcgatgtcg gacccgagca ccttctggat
    13201 gtgcatgctg acctcggggg tgaggcggtg cttgctgcca tcgatgtgcg agcgaaacgt
    13261 gacgccgtcg tc
//
LOCUS       DATLBZ010000436        11660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4239866, whole genome shotgun sequence.
ACCESSION   DATLBZ010000436 DATLBZ010000000
VERSION     DATLBZ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11660
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4239866"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VMZ53_22150"
     CDS             complement(<1..680)
                     /locus_tag="VMZ53_22150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUS31236.1"
                     /translation="MAAPQRVLVVDDNELYLRALQRQCRDEHAFTLTVVNNANDALAM
                     IASSKPDLVIMDMFMPSLDGVEACRQLKANPKTSEVPVIVATVDMSEELAAVCAAAGA
                     GAVSKPFCLKDLVPPTQTRVAADAPAVMTQTTETTRGADLLVGMLADAGVDVVFGLPG
                     GAISPVHDAMLGASMRLITTRHEAGAMFAAAGYAQSSGKLGVVAVTSGPGALNALTGL
                     ASAWCDSLP"
     gene            809..3193
                     /locus_tag="VMZ53_22155"
     CDS             809..3193
                     /locus_tag="VMZ53_22155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31237.1"
                     /translation="MSVDRDEVANLERTSRRLDAACAIIQAVHEELTIEGVLNGIVVN
                     LVEVAGFAGAEIAIDAHIDDLRVVHSTSAGSKGESSELTRECAVFLRGNEVGQLITRY
                     RTEELLEEQSDLLEFLLPTMYMGLEHAVSFAEVEDYRKSLEQKVIERTAQLNEAHEAL
                     KEAKASRDRFFANINHEIRTPLTLIQLSTDGIARSNEKLSKGTREKLESVNASTRRLL
                     HLVNSLLLLAAGDEGKLRIRPRPIDVAAILQKLVANWQPAAQVGEIQLSYAGPETCDA
                     TMDEAAIETIAGNFVSNATKFTPRGGRITVTLVAAEETVTISVRDTGPGIDPELVPRL
                     FGRFERAQSAVARGVRGTGIGLSLSKELVEQQGGAIDVRRESDPTGTTFEVTLARRQT
                     VEAIAVVPALPLPEQDYTAAQLDEKDTRTHAVTSLDTVDLPEARAEATHEPEATILLA
                     EDDPALQRNIADLLATRYRVLTASNGRIALEMAAEHLPDLLVTDLEMPEMNGVELTKR
                     FLQLKGTGMSPVLIVSAHAGLGTRLAGFEAGAVDYIVKPFAADELLARVRNQLAIRQL
                     AYKLHESQKLASLGMLSAGLAHEIRNPANALVNALRPLLELLPPEEQAPESTGALLAE
                     VALDAANQIRQRCMNILDYSRSDDVTRRPEHIDKLIGRACRLLTDSLKSVELRQEVNV
                     TTPVPCAGPLIEQILINLLDNAAYAAGVGGWVRVAGRQDAKRVIIEIGDSGPGVPAHI
                     QDRIFEPFFTTKPAGKGTGLGLAISRRIALNHGGDLRVVRLDQGTAFRLELPLE"
     gene            3284..4465
                     /locus_tag="VMZ53_22160"
     CDS             3284..4465
                     /locus_tag="VMZ53_22160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31238.1"
                     /translation="MAGAPAPEAKPLIVYVDDERPNRIVFEANLKQEFNIRSCADAES
                     ALKVLDSEDVAVLVTDIRMEGMDGLELLRIAKDKHPQTLRMVITAYSDIDPILRAINE
                     GLVARYIVKPWVREELIQVLRWATEVWTFGRDRAELMERLLQTERLASLGGIAAAYAH
                     DLKTPLMMVDVTLDQLREVAESVPALHEAIENTDLDEATKRQLLDQIDMAPELVEDTK
                     KASEDLKGIIALFNEFIKPGSKRGTAPVIDPLPIVRHVVLMNQKITGASIPIGYVGPA
                     QLPHIRMIPVELTQVLLNIIGNARQAVEARGEPNKGVAVDARMQGNMLELRVKDEGVG
                     MPPEVLKKVGTPWFTTRKEGTGLGLSNVQRLVGVAGGRMRIESEQGVGTVVTILLPTA
                     A"
     gene            complement(4469..6226)
                     /locus_tag="VMZ53_22165"
     CDS             complement(4469..6226)
                     /locus_tag="VMZ53_22165"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS31239.1"
                     /translation="MPFSSAHPALRRALAARSYSEPTPVQAAVLAEDAGEKDLLVSAQ
                     TGSGKTVAFGLALAPRLLGEAERLDKPGAPRALVICPTRELAQQVHRELSWLYAETGA
                     RMTTCVGGMDPRRERFELERGVHFVIGTPGRLCDHLERGALQLAKIDAVVLDEADEML
                     DMGFREDLERLLGAMPEERRTLMFSATIPPPIAQLAKRYQKKALRIAATGEGDRHRDI
                     EYHAVTIVPRERGLAVVNLLRYHDAPGALVFCSTRDGVTQAAAALSERGFSCAALSGE
                     LSQRERNQALQALRDGRARVCVATDVAARGLDLPDLGLVIHADLPQNAQTLVHRSGRT
                     GRAGKHGLAILVVPEPARRYVERMLQTAKIDASWRQPPSAEDIRVRDHDQLVLEIRAM
                     ADEPSDEDRKVAAQLLAERKAEDLVAALVRIRREARPAPEELTPPRTGPGGKKAPPPR
                     KEKPDRPERPDRQERRVENTGNPNGPDRRRVNKGSGEGRGTHGNPGGREFRGGKNAGG
                     VWFTINVGRSKNADPKWLIPLLCRRGDVTKRAIGKIQILMRETRVEIAPDAADHFAQA
                     VRRPDDKDKNVHIEPVDTE"
     gene            6323..7657
                     /locus_tag="VMZ53_22170"
     CDS             6323..7657
                     /locus_tag="VMZ53_22170"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS31240.1"
                     /translation="MKRLLSVALLACMAQTAHAEDPKINYQKFELPNGMRVYVIEDHK
                     APTAYGVTWFKVGSKDEIANRTGFAHLFEHLMFKGSAHLPDGLMDQLLEAAGGWSNAF
                     TSSDMTVYQNVANANFLEQMLWIDADRLAGLLDTFDKPKLDNQRDVVLNERRQSYENQ
                     PYGNAELFIQENLWPKDFGYHWSTIGYPQDLKAAAVPDVADFFKKYYVPNNATMVVAG
                     DVKFADVKKLVEKYFGWIPKGADPVRPTYKAPPPITKEIVVNTTDDVQVPRVYLVWRG
                     PSQFTGDEAPLDIAATLLGDGKSSRLYKRLVYDEKIAQDVSASLHAETLAGTFEIEVT
                     AKPGVDPKRLIKEISEEVAKLSSTAPDAKEVERAQNAYEAAFLGRLENTLQRAIQLAE
                     YDVAANDPDYFAKDLARFRAVTPAQVKDMAAKYLKPTARVTLTISPGKKVVK"
     gene            7657..9180
                     /locus_tag="VMZ53_22175"
     CDS             7657..9180
                     /locus_tag="VMZ53_22175"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS31241.1"
                     /translation="MRRSIALIVSLFASTAFAQPKLQAPPAPPQAKQPPPPAAPSGAP
                     WDKSGIKDWTKVPAPTKEPTFKPPVPKRSKLKNGMSLLLIENHALPIVSMAMVVPSAG
                     AAVDPTAKAGLASFTADLLDEGAGDLSAIQIAEEQDRLGASIRAYADVDSAGVSVSTL
                     TKTLEPTVDLVTKIVTQPKFDAKEFERVKGDRMTSLELRRDRPREVAAIMLNAALYGA
                     DSAYGHPASGMRETFKDIAVGDVQTFYKERWNPAAMTLIVAGDFDAKALKTKLDATLG
                     AWKPTGAKKPEKIAAKPAALGKRLLLADRKDSAQSDVRIGLVGIDRKDKRYYQLEVLA
                     NALGGSFTSRLNNRLREQLGITYGVRAGEDYRAQPGPFLISTAIVTPSTGQGLSETIK
                     ILDDLAANDLPKDELEKAKQNLIRALPAQFDTNAATVASYADLLTYGLPDNWYASYAT
                     NIKKVNAAQVKAAAKTLIPSKSFVVSIVGDLSKIKADIDKLGFGDALMHDLYGQPAK"
     gene            complement(9181..10614)
                     /locus_tag="VMZ53_22180"
     CDS             complement(9181..10614)
                     /locus_tag="VMZ53_22180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUS31242.1"
                     /translation="MTSKSASLVALVSGVVIGAQAHAQPAPGVHDSNEPHDGITESAE
                     RQPGSTRVEASTSAGTDAARHAELRASARRGSVDLSLTGGVDDRADRQQRGAVVAIDR
                     ASGTSRLAVTGGISDVALDLAIASVGIQTRSGGAAWSSGRIDLGNLDHELVIGASFTS
                     TSGMSTDTSEDLMHMGVLQRERRSDHRFLQAYLKDTLHVIETLDVSGGFVFDDWRNLS
                     AIETIRYGTDEQMDVHAPHIEDMHFSPQLGALYRATDHIAVRASGYRELRAPTVGELY
                     VPLVLDGAVAAANAQLTPESVWAGEVGPELRIGHLQARSAAFLHEIDSPIASANGERT
                     NLDHGRVLGVATEASWRPVEPVLAAVSYTRSSSTIRDAAYAGHELSLVPRHRAAALLS
                     YDARRVATLSGGVRWLGRQYIDAENTRAIRSTTLVDATATRAIRRGLAGYVSVENLLD
                     RRTIAAHDLPAAGRTVQIGVKLDSARF"
     gene            complement(10716..>11660)
                     /locus_tag="VMZ53_22185"
     CDS             complement(10716..>11660)
                     /locus_tag="VMZ53_22185"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HUS31243.1"
                     /translation="ILSIGLAAAGCKKSSPSPGPTGGGGGGGGGGGGGGGGGWLIGDS
                     GLMINMDGDKAYDDSIGGDENLNAIACRYAGEAWVVGDRGTLLYTNNGGEDWEAQVLP
                     TSARLRTVATQDDGPVFLGGDGAFLVTRDTGKTWTLLGDGTASFRSMSAAYAKTGVLA
                     LAEDGGLWEYEAGALTKKMTLPGARAVHQTTAGDVVMTAGSGIMRSMNGGATFEPLVV
                     DPGIIFDDIRVNDDGSATAVGAGGAIANIDAAGTVAVQYVGAADLHTIHIHHDTNGYA
                     GGDDGTVLITHDLGLTWQVGPNVGRTVRGVDEIGFGHR"
BASE COUNT         2120 a         3669 c         3934 g         1937 t
ORIGIN      
        1 ggcaagctgt cgcaccatgc agacgcgagt ccggtcagcg cgttgagcgc accggggccg
       61 ctcgtcaccg cgaccacacc gagcttgcca gacgactgcg cataccccgc agccgcgaac
      121 atcgcacccg cttcgtgacg cgtggtgatc agccgcatcg acgcgccgag cattgcgtcg
      181 tgcaccgggg aaatcgcacc acccggcagg ccaaagacaa catcgacgcc tgcgtcggcg
      241 agcataccaa ccaacagatc cgcgccgcgt gtggtttctg tcgtctgtgt catcacagcc
      301 ggcgcatcgg ccgcgacgcg agtttgtgta gggggcacca gatcttttag acaaaacggc
      361 ttagagactg ccccggcgcc cgcggcagcg cagaccgctg cgagctcttc ggacatgtcg
      421 acggtcgcaa cgatgacagg tacttcggaa gtcttgggat ttgccttcag ttgtcgacag
      481 gcctcgaccc cgtcgagaga cggcatgaac atgtccatga ttacgaggtc cggcttgctc
      541 gacgcgatca tggctagcgc gtcgttcgca ttgttaacga cggtgagcgt gaacgcgtgt
      601 tcgtcgcgac actgcctctg gagcgctcga agatagagct cgttgtcgtc gacgacgagg
      661 acacgttgcg gcgccgccat ccgggcctta tagacccgcc cgggcgattt caaaacgaga
      721 ttgtgcgacc gaggcggatg cgcacgaacg cgattcgcca gagtccgctt cacatcgtgg
      781 aaaccgcagg atgctagagt gacgctccgt gtcagtcgat cgcgatgagg tcgcgaacct
      841 cgaacggacc tcgcgtcggc tcgatgccgc gtgcgcgatc attcaggcgg tccacgagga
      901 gctgacgatc gagggcgtcc tcaacggcat cgtcgttaac ctcgtcgaag tggccgggtt
      961 tgctggcgcc gaaatcgcga tcgacgccca tatcgacgac cttcgtgttg ttcattccac
     1021 gtcggccggc tccaagggcg agagcagcga gctcacccga gagtgcgcgg tcttcctgcg
     1081 cggcaacgag gttgggcagc taatcacgcg gtaccggacg gaagagttgc tcgaagagca
     1141 gtccgatcta ctcgagtttc tactgccgac catgtacatg ggtctcgagc acgctgtctc
     1201 gttcgcggag gtagaggact accgcaagtc gctcgagcag aaggtcatcg agcgcaccgc
     1261 acaactcaat gaggcgcatg aagcgctcaa agaagccaag gcttcacgcg atcgattctt
     1321 tgccaacatc aaccatgaga tccggacccc gctcacgcta attcagctct cgaccgacgg
     1381 catcgcccga tcgaacgaaa agctgagcaa ggggacgcgc gaaaagctcg aatcggtgaa
     1441 cgccagcact cggcgcttgc tccacctggt gaatagcttg ctgcttctcg cggcgggcga
     1501 cgaaggaaag cttcgcatcc gaccccggcc gatcgacgtc gcggccattc tccagaagct
     1561 cgtcgcgaat tggcagccag ccgcgcaggt cggcgagatc cagctgagct atgcgggccc
     1621 cgagacgtgc gatgcaacca tggacgaagc ggccatcgag acgatcgccg gcaactttgt
     1681 ctcgaacgcg acgaagttca cgccgcgcgg gggccggatc acggtgacgt tggtcgcagc
     1741 cgaggagacg gtgacgatca gcgttcgcga taccgggcca ggcattgacc cggagctcgt
     1801 gccgcggttg ttcggacggt tcgagcgcgc gcagagcgcg gttgcgagag gcgtacgtgg
     1861 taccggcatc ggtttgtcgc tgtccaagga gctcgtcgag cagcaaggag gcgcgatcga
     1921 tgtgcggcgc gagagcgatc caacggggac aacgtttgaa gttacgctag ctaggagaca
     1981 gacggtcgaa gcgatcgccg tcgtgcctgc gctcccgcta cccgagcagg actacacagc
     2041 cgcgcaactc gatgagaagg acactcgcac gcacgcggtg acgagcctcg atacggtgga
     2101 cctgcctgag gcgcgcgccg aggcgacgca cgagcccgaa gcgacgattt tgctcgcgga
     2161 ggatgatccg gcgctgcagc ggaatatcgc ggacctgttg gcgacgagat atcgcgtgct
     2221 gaccgcgtcg aatgggcgca tcgcgctcga gatggcggcg gagcatctgc cggacttgct
     2281 tgtcaccgac ttggagatgc cggagatgaa cggcgtcgag ctgacgaagc ggtttctgca
     2341 gctgaagggg acggggatgt cgcccgtgct gatcgtcagc gcgcatgctg gattggggac
     2401 gcggctggcg ggcttcgagg cgggcgccgt cgattacatc gtgaagccgt ttgcggcgga
     2461 tgaattgctg gcgcgcgtgc ggaatcagct cgcgattcgt cagctcgctt ataagctgca
     2521 cgagagtcag aagctcgcgt cgcttggaat gctgtcggcg gggttggcgc acgagattcg
     2581 gaatcctgcg aacgcgctcg tcaatgcgtt gcggccgttg ctcgagctgt tgccgccaga
     2641 ggagcaggca ccggagtcga cgggcgcgct gttggctgag gtcgcgctcg atgcggcgaa
     2701 tcagatcagg cagcgctgca tgaacatctt ggattactcg cggtcggatg atgtgacgag
     2761 gcgaccggag cacatcgata agttgatcgg gcgtgcgtgc cggctgctga cggattcgtt
     2821 gaagagcgtc gagctgcggc aggaggtgaa cgtgactacg cccgtgccgt gcgccggacc
     2881 gctgatcgaa cagattctga ttaacctcct ggataatgcg gcgtatgcgg cgggggtggg
     2941 cggatgggtc cgcgtcgcgg ggcggcagga tgccaagcgc gtcatcatcg agatcggcga
     3001 cagcgggcct ggggtgccgg cgcatatcca agaccgcatc ttcgaaccgt tctttacgac
     3061 caaaccggca ggaaagggca cggggctggg gctcgcgatt tcgcggcgga ttgcgctcaa
     3121 ccacggcggt gacttgcgcg tcgttcggct cgatcagggg accgcgtttc ggctcgagtt
     3181 acctctcgaa taacccaaac gtgtaggtgg ccgcgcacgg tgacgcaccg cttcagaaat
     3241 tggggcgaac ggccgaacca gatatcatta attgataacg cgaatggcag gggcgcccgc
     3301 accagaagcg aagccgctga tcgtctacgt cgacgatgag cgaccgaatc gcatcgtctt
     3361 tgaggcgaac ctcaagcagg agttcaacat ccgcagctgc gcggatgccg agtctgcgct
     3421 gaaggtgctc gactccgaag atgtcgcggt gctcgtgacg gacatccgca tggaagggat
     3481 ggacggtctc gaactgctgc gcatcgcgaa ggacaagcat ccgcagaccc tgcgcatggt
     3541 gatcaccgcg tactcggaca tcgatccgat cttgcgcgcg atcaatgagg ggttggttgc
     3601 gcgctacatc gtgaagccgt gggttcgcga agagctcatt caggtgctgc gttgggcgac
     3661 ggaagtgtgg acgttcggcc gcgatcgcgc ggagctcatg gagcgcctgc tgcagaccga
     3721 gcgacttgca tcacttggcg ggattgccgc ggcgtatgcg catgacttga agacgccact
     3781 catgatggta gacgtcacgc tcgatcaatt gcgcgaggtc gcggagtcag tccctgcgct
     3841 gcacgaggcg atcgagaaca ccgatctcga tgaggccacg aagcgccagc tgctcgatca
     3901 gatcgatatg gcgcccgagc tcgtcgagga cacgaagaag gcgtccgaag atctgaaggg
     3961 catcattgcc ctcttcaacg agttcatcaa accgggtagc aagcgcggaa cggcacccgt
     4021 gatcgatccg ttgccgatcg ttcgccatgt cgtgctcatg aaccagaaga tcaccggcgc
     4081 gagcattccg atcggttatg tcggaccggc gcagctgccg catatccgca tgattcccgt
     4141 cgagcttacg caggtgctgc tcaacatcat cggcaatgcg cgtcaggccg tcgaagcgcg
     4201 cggcgagccg aacaagggcg tcgcggtgga tgcgcgtatg cagggcaaca tgctcgagct
     4261 gcgcgtgaag gacgagggcg tcgggatgcc gcccgaagtg ctgaagaagg tcggcacgcc
     4321 gtggttcacg acgcgcaaag aaggcacggg ccttgggctg tcgaacgtgc agcggctcgt
     4381 cggcgtagcc ggtggtcgca tgcggatcga gagcgagcag ggcgtcggga cggtcgtgac
     4441 gattctgctg ccgaccgccg cgtagcgcct actcggtgtc gaccggctcg atgtggacgt
     4501 tcttgtcttt gtcgtccgga cgccggacag cttgcgcgaa gtgatcagcg gcgtcagggg
     4561 cgatctcgac gcgcgtttcg cgcatcagga tctggatctt gccgatcgcg cgtttcgtca
     4621 cgtcgccgcg ccggcagagc agcgggatga gccacttcgg atcagcattc ttcgagcggc
     4681 ccacgttgat cgtgaaccac acgccgcccg cattcttgcc accgcggaac tcgcgaccgc
     4741 cgggattgcc gtgcgtgccg cgaccctcgc cgctgccctt gttgacgcga cggcggtctg
     4801 gcccgttcgg attgcccgtg ttctcgacgc ggcgttcttg gcgatcgggc cgctcgggcc
     4861 ggtctggctt ctctttgcgc ggcggcggtg ccttcttgcc gcccggaccg gtgcgaggag
     4921 gcgtgagctc ttctggcgca ggacgcgctt cgcgacgaat gcgcacgagt gcggcgacga
     4981 ggtcttcggc tttgcgctcg gcgagtagct gtgcggcgac cttgcgatct tcgtcggagg
     5041 gctcgtccgc catcgcgcga atctcgagca cgagctgatc gtggtcgcgc acgcggatgt
     5101 cttcggccga tggcggctgc cgccacgacg cgtcgatctt cgcggtctgc agcatccgct
     5161 cgacgtagcg gcgcgcgggc tccggcacga cgaggatcgc gaggccatgc ttgccagcgc
     5221 gacccgtgcg accgctgcga tgcacgagcg tctgcgcgtt ctgcggaagg tcggcgtgaa
     5281 tgacgagacc gaggtccgga aggtcgagac cgcgcgcggc gacgtcggtc gcgacgcaca
     5341 cgcgtgcacg gccatcgcgc agcgcttgca gcgcttggtt gcgctcacgt tgcgagagct
     5401 cgccggatag agcagcgcag gagaagccac gctcgctgag agcggcggcg gcctgcgtga
     5461 caccgtcgcg cgtcgagcag aacacgaggg cgccgggcgc atcgtggtag cgcagcaagt
     5521 tcacgacggc gaggccgcgc tcgcgcggga cgatcgtgac ggcgtggtac tcgatgtcgc
     5581 gatggcgatc gccttcgccg gtggcggcga tacggagcgc cttcttctga tagcgctttg
     5641 cgagctgcgc gatcggcggc gggatcgtcg ccgagaacat cagcgtgcga cgctcttcgg
     5701 gcatcgcgcc gagcaaccgc tcgaggtcct cgcggaaccc catgtcgagc atttcgtcgg
     5761 cttcgtcgag cacgaccgcg tcgatctttg cgagctgcag cgcgccgcgc tcgaggtgat
     5821 cgcagagccg gcctggcgta ccgatgacga agtgcacgcc acgctcgagc tcgaaccgct
     5881 cgcgccgggg atccatgccg ccgacgcacg tggtcatgcg cgcgcccgtc tcggcgtaga
     5941 gccacgacag ctcgcggtgg acttgctgcg cgagctcgcg ggtcgggcag atcacgagcg
     6001 cgcgaggggc accgggcttg tcgagccgct cggcttcacc gaggaggcga ggcgcgagcg
     6061 cgaggccgaa cgcgaccgtc ttgccggagc ccgtctgggc cgagaccaac aggtcttttt
     6121 ctccggcgtc ctcggcgaga acggcggctt gaaccggggt cggttcggag taggagcggg
     6181 ctgcgagggc tcgacgcagc gcgggatgcg cagaggaaaa cggcaaggcg gcgcacccta
     6241 gtcggtctgc cggagggagg caaatgctgt cgcacgctgt cgcagctttt tgttctctgg
     6301 ggccggtgtt agcgtccgcc gcatgaaacg gctgctctcc gtggctctcc tggcgtgcat
     6361 ggcgcaaacc gcccacgcgg aggaccccaa gatcaactac cagaagttcg agctccccaa
     6421 cggcatgcgg gtctacgtga tcgaggatca caaggccccg accgcgtacg gcgtgacctg
     6481 gttcaaggtc ggcagcaagg acgagatcgc aaaccgcacg gggttcgcgc acctgttcga
     6541 gcacctgatg ttcaagggct cggcgcacct gcccgacggc ctcatggatc agctcctcga
     6601 agcggccggc ggttggtcga acgccttcac gtcgagcgac atgacggtct atcaaaacgt
     6661 cgcgaacgcg aacttcctcg agcaaatgct ctggatcgat gccgaccgcc tcgcgggtct
     6721 gctcgatacg ttcgacaagc ccaagctcga taaccagcgc gacgtcgtgc tcaacgagcg
     6781 ccgccagagc tacgagaacc agccgtacgg caacgccgag ctgttcattc aagagaacct
     6841 gtggccgaaa gacttcggct accactggtc gacgatcggt tacccgcagg atctcaaggc
     6901 agcagcggtt cccgacgtcg cggacttctt caagaagtac tacgtgccga acaacgccac
     6961 gatggtcgtc gcgggcgacg tgaagttcgc cgacgtgaag aagctcgtcg agaaatactt
     7021 cggctggatc ccgaagggcg cggaccccgt acgtccgacg tacaaggcgc cgccgccgat
     7081 cacgaaagag atcgtcgtca acacgaccga cgacgtccaa gtgccgcgcg tgtatctcgt
     7141 gtggcgcgga ccgtcgcaat tcacgggcga cgaagccccg ctcgatatcg cagcgacgct
     7201 cctcggcgac ggcaagtcga gccgcctcta caagcgcctc gtgtatgacg agaagatcgc
     7261 gcaggacgtg tcggcgtcgc tccatgccga gacgctcgca ggcacgttcg agatcgaagt
     7321 gacggcgaag ccgggcgtcg atccgaagcg gctcatcaaa gagatcagcg aagaggttgc
     7381 gaagctgtcg tcgacggcgc ccgatgccaa agaagtcgag cgtgcgcaga acgcgtacga
     7441 ggctgcgttc ctcggtcgcc tcgagaacac gctgcagcgc gcgatccagc tcgccgagta
     7501 cgacgtggct gcgaatgacc cggattactt cgcgaaggac ctggcgcgct tccgcgccgt
     7561 gacgcctgcg caggtgaaag acatggccgc gaagtatttg aagccgaccg cgcgcgtcac
     7621 gctgacgatc agccccggca agaaagtggt gaagtgatgc gccgctcgat cgcactcatc
     7681 gtttcgctgt tcgcctcgac ggcattcgca cagccaaagc tccaagcgcc gcctgcaccg
     7741 ccgcaggcga agcaaccacc gccgcccgca gcgcctagtg gtgcgccgtg ggacaagtcc
     7801 ggcatcaaag actggacgaa ggttccagct ccgacgaagg agccgacgtt caagccgccc
     7861 gttcccaagc gcagcaagct gaagaacggc atgtcgctgc tgctcatcga gaatcatgcg
     7921 ctgccgatcg tgtcgatggc gatggtcgtg ccgagtgccg gcgcggcggt cgatccgacg
     7981 gcgaaagcgg ggctcgcttc gttcaccgcc gatctgctcg acgagggcgc aggtgatctc
     8041 agcgcgatcc agatcgcgga agagcaagac cgcctcggtg cgagcattcg cgcgtacgcg
     8101 gatgtcgaca gcgcgggcgt cagcgtctcg acgctgacga agacgctcga gccgacggtc
     8161 gatctcgtga cgaagatcgt cacgcagccg aagttcgacg cgaaagagtt cgagcgcgtg
     8221 aagggcgatc ggatgacgtc gctcgagctg cgccgcgatc ggccgcgcga ggtcgctgcg
     8281 atcatgctga acgctgcgct ctacggtgcc gattcggcgt atggccatcc cgccagcggc
     8341 atgcgcgaga cattcaagga catcgccgtc ggtgacgtgc agacgttcta caaagaacgc
     8401 tggaaccccg ctgcgatgac gctgatcgtc gcaggtgact tcgatgcaaa agcgctcaag
     8461 acgaagctcg acgccaccct cggcgcgtgg aagccgaccg gtgcgaagaa gccagagaag
     8521 atcgcggcga agccggccgc gctcggtaag cggctcctgc tcgccgatcg caaggactcg
     8581 gcgcaatccg atgtgcgcat cgggctcgtc ggcatcgatc gcaaagacaa gcgctactac
     8641 cagctcgagg tgctcgcgaa cgcgctcggc ggtagcttca cgagccgcct caacaatcgc
     8701 ttgcgcgagc agctcggcat cacgtacggc gtccgcgccg gcgaggatta ccgagcgcag
     8761 cccggcccgt tcctgatctc gaccgcgatc gtcaccccgt cgacggggca ggggctcagc
     8821 gagacgatca agatcctcga cgatctggcg gcgaatgatc tgccgaaaga cgagctcgag
     8881 aaggcgaagc agaatctcat ccgagcgctg ccggcgcagt tcgacacgaa cgcggccacg
     8941 gtcgcgtcgt acgccgatct cctgacgtac ggccttccgg acaactggta cgcgagctac
     9001 gcgacgaaca tcaagaaggt caacgcggcg caggtgaagg cggccgcgaa gacgctgatc
     9061 ccgtcgaaga gcttcgtcgt gtcgatcgtc ggcgacctgt cgaagatcaa agccgacatc
     9121 gacaagctcg gcttcggcga cgcgctcatg cacgacctct acggccagcc ggcgaaatag
     9181 ctagaaccgc gcgctatcga gcttcacgcc gatttgcacg gtgcggcccg cggccggcaa
     9241 gtcgtgcgcg gcgatcgtgc ggcgatcgag caggttctcg acgctgacgt agccggcgag
     9301 gccgcggcga atcgcgcgcg ttgcagtcgc atcgacgagc gtcgtcgagc gaatcgctcg
     9361 cgtgttctcg gcatcgatgt actggcggcc gagccagcgc acgccgccgg aaagcgtcgc
     9421 cacgcgccga gcatcgtacg agagcagcgc cgctgcgcga tggcgaggga caagggagag
     9481 ctcgtgaccc gcatatgccg cgtcgcgaat cgtcgagctc gaacgggtgt acgagacggc
     9541 ggcgagcacc ggctcgacgg gacgccagct ggcttccgtc gcgacgccga ggacgcggcc
     9601 atgatcgagg ttggtgcgct cgccgtttgc gctcgcgatc ggagaatcga tctcgtggag
     9661 gaacgcggcg ctgcgcgcct gcaggtgccc gatgcgcagc tcgggaccga cttcgccagc
     9721 ccacaccgat tcgggcgtga gctgcgcgtt cgccgcggcg acagcaccgt cgaggacaag
     9781 cgggacgtac agctcgccga cggtcggcgc gcgcagctcg cgatagcccg aggcgcgtac
     9841 ggcgatgtga tcggtcgcgc ggtagagggc gccgagctgc gggctgaagt gcatgtcctc
     9901 gatgtgaggc gcgtggacgt ccatctgctc gtccgtgccg tagcgaatcg tttcgatcgc
     9961 gctgaggttg cgccagtcgt cgaatacgaa gccgccgctg acgtcgagcg tctcgatcac
    10021 gtggagcgtg tctttgagat acgcctggag gaagcgatgg tccgagcggc gctcgcgctg
    10081 cagcacgccc atgtgcatga gatcttccga cgtatcggtc gacatgccgc tcgtgctcgt
    10141 gaagctcgcg ccgatgacga gctcgtgatc gaggttgccg aggtcgatgc gacccgacga
    10201 ccacgcggcg ccgccgctgc gcgtctggat accgacgctc gcaatcgcga gatcgagtgc
    10261 gacgtcagag atgccgcccg tgacggcgag ccggctcgtg ccgcttgcgc gatcgatcgc
    10321 gacgactgcg ccgcgctgct ggcgatcggc gcgatcgtcg acgcctccgg tcagcgagag
    10381 atcgacgctg ccgcgtcgtg cactggcgcg gagctctgca tggcgtgccg cgtccgtacc
    10441 cgcgctggtg ctcgcttcga cgcgcgtgct gccaggctgg cgctccgcac tctcggtgat
    10501 tccgtcgtgc ggctcgttcg agtcgtgcac ccccggcgcg ggttgcgcgt gagcctgcgc
    10561 gccgatgaca acacccgaaa cgagcgcgac gagggaggcc gacttggagg tcacgcctac
    10621 cctcgctttg catccggcgc gccacgcgcg gaagctcact caccgagcgt gcgccgagga
    10681 gacagcgtgg cgatgaggag aatgtcgtcg acgatctagc ggtgaccaaa cccgatctcg
    10741 tcgacaccgc gcacggtgcg cccgacgttc gggccgactt gccacgtgag accaaggtca
    10801 tgcgtgatca agacggtgcc gtcgtcgccg ccagcgtagc cattcgtatc gtggtggatg
    10861 tggatcgtgt gcaggtctgc agcgcccacg tactggactg ccacagttcc tgccgcgtcg
    10921 atgttcgcga tcgcgccacc ggcaccgacg gccgtcgcac tgccgtcatc gttgacgcgg
    10981 atgtcgtcga agatgatgcc cggatcgacg acgagcggtt cgaacgtcgc gccgccgttc
    11041 atcgaccgca tgatgccgct gcccgcggtc atgacgacgt cgccagcggt cgtctggtgc
    11101 actgcgcgcg caccggggag cgtcatcttc ttcgtcagcg cacctgcctc gtactcccag
    11161 agcccgccgt cttcggcgag cgcgagcacg ccggtcttcg catacgccgc cgacatcgag
    11221 cggaagcttg cggtgccgtc gccgagtagc gtccatgtct tgccagtatc ccgcgtgacg
    11281 aggaacgcac cgtcgccgcc gaggaagacc gggccgtcat cttgcgtcgc gaccgtgcgc
    11341 aggcgagccg acgtcggcag cacttgtgct tcccaatcct cgccgccgtt gttcgtatag
    11401 agcagcgtgc cgcggtcgcc gacgacccac gcttctcccg catagcgaca tgcgatcgcg
    11461 ttgaggttct cgtcgccgcc gatgctgtcg tcgtaggcct tgtcgccgtc catgttgatc
    11521 atcaggccgg agtcgccgat cagccagcct ccgccgccac cgccgccacc gccaccacca
    11581 ccaccgccgc caccgccggt gggaccgggg ctcggcgagc tcttcttgca gccagcggcc
    11641 gcgagaccaa tcgaaagaat
//
LOCUS       DATLBZ010000437         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9484053, whole genome shotgun sequence.
ACCESSION   DATLBZ010000437 DATLBZ010000000
VERSION     DATLBZ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9484053"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VMZ53_22190"
     CDS             <1..1977
                     /locus_tag="VMZ53_22190"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUS31244.1"
                     /translation="PTETMSARHVLPLIVVLVAFAFVQCGHRDVAVSEHSSDGQSGEQ
                     QLGAQQIDIDWPQAPGDAHFDVQDPPRTIEELRARLQRILERERIAGMSIALVDRNGT
                     MWVGGLGVRDRATRERVDADTAFRVGSLSKSIIALGVMRLVDQGKLDVDRPLREIIPD
                     LEIDNPWEDTAPITLAQCLEHTAGFDDLRFNEIFAPDETTTVRQALALNPRSRVIRWK
                     PGTRHAYSNVGFTVAALAIEKAAGEPFDVYLRREILAPMGIVDADFRRTETIAPRLAT
                     GYMSFDEPVVFRAVAHRPPGALLASASDVAKLVHFWIVRGVGYPPIISAAGLARIERS
                     GTLPYPHTDAEYGFANYTDVWSPVIGRGHDGGMPGFHASYRYFPGLDVGYVMLLNSSY
                     VFRGYFEIRALLFSYLTQGRTFPPPAATANAAEPPGAPYFALGTKNGLFGFMDTLWIG
                     FHVSAMPDGTLVVRSLQGDTGLLVPAADGGYLHPGAAGSSVRFTTNADGTPIMLLGWQ
                     YTEARPMWLGVGRYAALRTTTALLVLAPLWALTELLLAFIFRRRILPASLLIWPSIAG
                     ASSFAISHLLGAAFFAGVIGVVHPLTVGLCAATIVLAVASAATLVCAVRWLRRPDRPP
                     LIAMILPLLFGLAFSGFSLWLAIHGVIGIRTWSW"
     gene            1996..>2547
                     /locus_tag="VMZ53_22195"
     CDS             1996..>2547
                     /locus_tag="VMZ53_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31245.1"
                     /translation="MRMLALLVVIAACTSSKPTATAPPQGSSGSGSVATSDAAAMESG
                     TATTGSAASMPDAAVAAAIDAGSLDDDLGMTYTDPAGGSPGTVKANTVYRSDCSTTHP
                     IAHDVCDGAPPDTRKLKSCKALHVKMFKPCKKGAPSCYVERTCADGRVVASDFLECAA
                     EQSHRCFTKSSRIYKDDIAYLSEP"
BASE COUNT          399 a          862 c          835 g          451 t
ORIGIN      
        1 cccacggaaa cgatgtccgc gcgccacgtg ctcccgctga tcgtagtgct cgtcgcattc
       61 gcgttcgtgc aatgcgggca tcgagacgtt gcggtgagcg agcattcgag cgacggccag
      121 agcggcgagc agcaactcgg cgcgcagcag atcgacatcg actggccgca ggcaccgggc
      181 gacgcgcact tcgacgtgca ggatccgccg cgcacgatcg aggagctgcg cgcgcgactc
      241 cagcgcatcc tcgagcgcga acggatcgcg ggcatgagca tcgccctcgt cgatcgcaat
      301 gggacgatgt gggtcggcgg gcttggcgta cgtgaccgcg ccacgcgcga gcgtgtcgat
      361 gcggacacgg cgtttcgcgt cggctcgctc tcgaagagca tcattgcgct cggcgtgatg
      421 cggctcgtcg atcaaggcaa gctcgacgtc gaccggccac tgcgcgagat cattccggat
      481 ctcgagatcg ataatccgtg ggaggacaca gcgcccatca cgctcgcgca gtgcctcgag
      541 cacaccgccg gctttgacga cttgcgcttc aacgagatct tcgcgccgga tgagacaacg
      601 accgtgcggc aggcactcgc gctgaacccg cggtcgcgtg tcattcgctg gaagccgggc
      661 acgcggcacg cgtattcgaa tgttggcttc accgttgcag cgctcgcgat cgagaaggcg
      721 gccggcgagc cgttcgacgt gtatctgcgc cgcgagattc tggcgccgat gggaatcgtc
      781 gacgcagact ttcgccgcac cgagacgatc gcgccgcgac tcgcgaccgg ctatatgagc
      841 ttcgacgagc ccgtcgtgtt tcgcgcggtc gcacatcggc cacctggcgc attgctcgcg
      901 tcggcaagcg acgtcgcgaa gctcgtccac ttctggatcg tgcgcggcgt gggctatccg
      961 ccgataatct ccgctgcggg gctcgcgcgc atcgagcgca gcggcacctt gccctatccg
     1021 cacaccgatg cggagtacgg cttcgcgaac tacaccgacg tgtggtcccc ggtgatcggc
     1081 cgcggtcacg acggcggcat gcccgggttt cacgcgagct atcgctactt ccccggcctc
     1141 gacgtcggct acgtgatgct cctcaactcg agctacgtgt tccgcggcta cttcgagatt
     1201 cgcgcgctgt tgttttcgta tctgacgcag ggccgcacgt tcccgccgcc agcagcgacc
     1261 gcgaacgccg ccgagccgcc gggtgcgccc tacttcgcgc tcggcacgaa gaacggcctg
     1321 ttcggcttca tggatacgct gtggatcggg tttcacgtca gcgcgatgcc ggacggaacg
     1381 ctcgtcgtac gcagcctcca aggcgatacg ggcctgctcg ttccagcggc tgacggcggg
     1441 tacctgcatc ccggggctgc gggcagcagc gttcgcttca cgacgaatgc cgacggcacg
     1501 cccatcatgt tgctcggctg gcagtacaca gaagcgcggc cgatgtggct cggcgtcggg
     1561 cgctacgcag cgctgcgaac aacaaccgcg ttgctggtgc tagcgccgct gtgggcgctg
     1621 acggagctct tgctcgcatt catcttcagg cgtcggattc tgcccgcgtc gctcctcatc
     1681 tggccatcga tcgcgggtgc ctcttcgttc gcgatttcgc atttgctcgg cgcggcgttc
     1741 ttcgcgggcg tcatcggggt cgttcatccg ctcaccgttg gcctttgcgc cgcgaccatc
     1801 gtgctcgctg ttgcctccgc ggcgacgctc gtctgcgcag tgcggtggct acgccgcccc
     1861 gatcgcccgc cgctgatcgc gatgatcctg ccgctcctct tcgggctcgc attctcgggc
     1921 ttctcgctgt ggctcgcgat ccacggagtg atcgggatcc gcacctggtc ctggtgactc
     1981 cgatataacc gccgcatgcg aatgcttgca ttgttggtgg tgatcgcggc gtgcacgagc
     2041 tcgaagccca cagcgaccgc gccgccccaa ggctcgagcg gcagcggcag cgtggcgacg
     2101 agcgatgcgg cggcgatgga aagtggtacg gcgacgacgg gaagtgcggc gagcatgccc
     2161 gatgccgctg tcgccgctgc gatcgacgcc ggctcgctcg acgacgatct cgggatgacg
     2221 tacaccgatc cggccggcgg ctcgccgggc acggtgaagg cgaacaccgt ctatcgctcg
     2281 gactgctcga cgacacatcc catcgcacac gacgtctgcg acggcgcccc acccgacacg
     2341 cgcaagctca agagctgcaa ggcgctgcac gtgaagatgt tcaagccttg taagaagggc
     2401 gcgccaagct gttacgtcga gcgcacgtgc gctgatggcc gcgtcgtcgc gagcgacttc
     2461 ctcgagtgcg ccgcggagca gagccaccgc tgcttcacga agtcgtcgcg catctacaag
     2521 gacgacatcg cgtacctcag cgagccc
//
LOCUS       DATLBZ010000438         5545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4251310, whole genome shotgun sequence.
ACCESSION   DATLBZ010000438 DATLBZ010000000
VERSION     DATLBZ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4251310"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VMZ53_22200"
     CDS             <1..1314
                     /locus_tag="VMZ53_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31246.1"
                     /translation="QRGPAEMGFRTNAARMNLNRDYVKADSLEIRAVLGVFRKYEPVV
                     SVDMHVTDGAKFEHDVAVLVGTFAKRDDGLDELSRTLSDQLGARLTALGHLPLAFYPS
                     FEKDDDPASGFSVSEAPLRFSQSYASARSRIGILLETHSWRPYKHRVETTYHVLQALF
                     EQAAKDAATWAKVEADAVAADQKLLGGQLPVAYENSTHVTQLQFRGYAYEQRASEISG
                     GMWTIYDETKPQIWNIPLLDEVVPKLSVRVPKEGYIIDGGFAAQLAPVLERHGIQYTK
                     LAPARLDVEVYRATKVTFDPPYEGRTRAKLEGAWKAETRTLDRGAIFVSLHQPNVRLI
                     VHLLDPDGPDSFAQWGFVQSAFEQKEYMEAYVAEEAAREMLAKDPSLKAAFDAALADP
                     DFAKSPEKRREFFYKRHPAWDERMNLLPIYRVDMPMTVTGSSARE"
     gene            complement(1316..2167)
                     /locus_tag="VMZ53_22205"
     CDS             complement(1316..2167)
                     /locus_tag="VMZ53_22205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31247.1"
                     /translation="MRCLLAAALCMGCATPARSAPDSDPYVEHTVALRKQLSGKKLGK
                     LQYRTEKPFVVVGDGSADELEHDALTVQWAVAMLEKDFFTTRPTKILDVYLFRNAKSY
                     ERGVKRLTGESPSTPYGFYSSTHEGLFMNIATGGGTLVHEIVHPYVESDFPNAPPWLN
                     EGLGSLFEQSAERGGHIIGLTNWRLAGLQSAIDEDTVPSFEKLTHYSTAEFYGDDSGV
                     NYAASRYLLYYLQEQGVLRNFYKTCRDARDKDPSCIGALKKVLGTDDLVAFKKKWQLY
                     VAQLTFP"
     gene            2259..2594
                     /locus_tag="VMZ53_22210"
     CDS             2259..2594
                     /locus_tag="VMZ53_22210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31248.1"
                     /translation="MLDVTNDTALPVVFGLQVGDTVAVQTDVLAELARHAPGLPPTWL
                     MLPAGTEGKLLGYRDRAGEPYALVEIALERKLVMIIREHKLVSAPRPAPPTPMHRRPS
                     RRAGSRHSR"
     gene            complement(2625..2975)
                     /locus_tag="VMZ53_22215"
     CDS             complement(2625..2975)
                     /locus_tag="VMZ53_22215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS31249.1"
                     /translation="MAVRLLLVEDHEDSADLLAQLLENNGYAVSIARCATDALSLAES
                     NTFDVVVSDVGLPDASGYELMTKLRGKMKGIAITGTSGDDAEARGREAGFSAHLTKPV
                     SVRRLQQALAELGI"
     gene            3098..3865
                     /locus_tag="VMZ53_22220"
     CDS             3098..3865
                     /locus_tag="VMZ53_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31250.1"
                     /translation="MKMLALLTTIFATATGCHTTSGPTAAAPSSPASLTQPTADLTQP
                     TADLAGRRAQMLAYLHDYTEAGQYPTDDAGLPRSVFVDAKGVRCPMAELIFRSGHADL
                     VDAVAREHNEVRLSDVNEGPLLAWMQSSGLTRDEINLVQGVADLDMVWIQEEAPSQET
                     ILARGQIRGRLESAERALRDGTTHSLQVAVAALPNGKLPEAKLAAGAKVVPVAAMKSG
                     PQGRPLRAVALAPRWRTSPQMVVAPNGSITLAPAVRN"
     gene            complement(3862..4236)
                     /locus_tag="VMZ53_22225"
     CDS             complement(3862..4236)
                     /locus_tag="VMZ53_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31251.1"
                     /translation="MTPLLCFVVFVSATLIDFAHARCIVAISCRAPHRAAMWSIVQWA
                     GATVGFIIAVKVTFWVLPFEAAGLYIGTVIAVATSKDDKPRSTVAEPMRSGRIPVELV
                     TTAMRKMSASTTASTATSSTWS"
     gene            4418..>5545
                     /locus_tag="VMZ53_22230"
     CDS             4418..>5545
                     /locus_tag="VMZ53_22230"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUS31252.1"
                     /translation="MIGLGCMRLSTDASRDDARSLAVLRVALDEGITLFDTADAYALD
                     DLDIGHNEKLLARALAEELRAHVGTSRVGTDGRAHVGTSLVATDGRAHVGTSLVATEG
                     RAHGGPPVVVTKGRAHGGTPVVVTKGGLVRPDGAWVPKGNARHLGLAARSSRERLASR
                     DATGAEGASGATSGNESAAPIDLYLLHVVDPRVPLATSVRALAKLRDDGIVRAIGLSN
                     VTQHQLEQALALTQIEAIEVELGPHKLDAFHSGLVDFCRQRDIRVLAHRPLGGPNGVK
                     RLARDKRLAQLCENANWSDRPSDAPEHSPDAEHSASASVQWPTIHELVLAWLAAKDVV
                     PIPGATRVETVRSIARANRLQLSEELVRELDAHLLDEPSTTR"
BASE COUNT          954 a         1882 c         1800 g          909 t
ORIGIN      
        1 cagcgcggcc ccgccgagat gggctttcgc acgaatgccg cgcggatgaa tctcaaccgc
       61 gactacgtca aggccgactc gctcgagatc cgggccgtgc tcggcgtgtt ccgcaaatac
      121 gagcccgtcg tgtccgtcga catgcatgtc accgacggcg cgaagttcga gcacgacgtg
      181 gcggtcctcg tcggcacgtt tgccaagcgc gacgatggcc tcgacgagct ctcgcgcacg
      241 ctctccgatc agctcggcgc gcgcctcacc gcgctcggcc acttgccgct cgcattctat
      301 ccgtcgttcg agaaggacga tgatccggcg tcgggcttct ccgtcagcga agcgccgcta
      361 cggttctcgc agtcgtatgc gagcgcgcgc tcgcgcatcg gcattctgct cgagacgcac
      421 agctggcgtc cgtacaagca tcgcgtcgag acgacgtatc acgtgttgca agcgctgttc
      481 gagcaagcgg cgaaggacgc ggccacgtgg gcgaaggtcg aggcggatgc tgtcgctgcg
      541 gatcagaagc tgctcggcgg gcagctcccg gtggcctacg agaacagcac gcacgtcacg
      601 cagctgcagt ttcgcggcta cgcgtacgag cagcgcgcgt cggagatttc gggcggtatg
      661 tggaccatct acgacgagac caagccgcag atctggaaca tcccgctcct cgacgaagtc
      721 gtcccgaagc tctcggtgcg cgtgccgaag gagggctaca tcatcgatgg cggcttcgcc
      781 gcgcagctcg cgccggtgct cgagcgccac ggcattcaat acacaaagct cgcgccggcg
      841 cgcctcgatg tagaggtcta tcgcgcgacg aaggtcacgt tcgatccgcc gtacgaaggc
      901 cgcacgcgcg cgaagctcga aggcgcctgg aaggcagaga cgcgaacgct cgatcgtggc
      961 gcgatcttcg tgtcgctcca tcagccgaac gtgcggctca tcgtccacct gctcgacccc
     1021 gacggtcccg actcgttcgc gcaatggggc ttcgtgcagt ccgcgttcga gcagaaggaa
     1081 tacatggagg cgtacgtcgc ggaggaagcc gcgcgcgaga tgctcgcgaa ggatccgtcg
     1141 ctgaaggccg cgttcgacgc cgcgctcgcg gaccccgatt tcgcgaagag ccccgagaag
     1201 cgccgcgagt tcttctacaa gcgacatccc gcgtgggacg agcgcatgaa cctgctgccg
     1261 atctatcgcg tcgacatgcc gatgacggtg accggatcgt cagcgcgcga gtgagtcatg
     1321 gaaacgtcag ctgcgcgacg tacagctgcc acttcttctt gaacgcgacg agatcgtcgg
     1381 tgccgagcac cttcttcagc gcgccgatgc agctcggatc cttgtcgcgc gcgtcgcggc
     1441 acgtcttgta gaagttacgg agcacgccct gctcttgcag gtagtagagc aagtagcgcg
     1501 atgccgcgta gttcacgccc gagtcgtcgc cgtagaactc ggcggtcgag tagtgcgtga
     1561 gcttctcgaa gctcggcacg gtgtcctcgt cgatcgcaga ctgcaagccg gcgaggcgcc
     1621 agttcgtcag cccgatgatg tggccaccgc gttccgccga ttgctcgaac agagagccga
     1681 gcccctcgtt gagccacggc ggtgcgttcg gaaaatccga ctcgacgtac ggatgcacga
     1741 tctcgtgaac gagcgtgccg ccgcccgtcg cgatgttcat gaacagcccc tcgtgcgtcg
     1801 acgagtaaaa gccatacggc gtgctcggtg actcgccggt caaccgcttc acacctcgct
     1861 cgtagctctt cgcgttgcgg aacagataga cgtcgaggat cttcgtcggg cgcgtcgtga
     1921 agaagtcttt ctccagcatc gcgacggccc actgcacggt cagcgcgtcg tgctcgagct
     1981 cgtccgcgga accatcacca acgacgacga acggcttctc ggtccgatac tgcagcttgc
     2041 cgagcttctt gccgctcagc tgcttgcgca gcgcgacggt gtgctcgacg tacggatcgc
     2101 tgtcgggcgc agagcgggcg ggcgtcgcgc atcccatgca gagcgcggcc gcgaggagac
     2161 acctcatgtc agtacgacac gccatcgccg tgctcgttgc atagcgcact cccgatcgct
     2221 gcgccgggac tgtcagatcg gacggttacc gtgcgcgcgt gctggacgtt acgaacgaca
     2281 ccgccttgcc tgtcgtgttc ggactgcagg tcggggacac cgtggccgta caaaccgacg
     2341 tgcttgccga gctcgctcgt cacgcgccgg gcttaccgcc gacgtggctc atgcttccgg
     2401 cggggaccga aggcaagctg ctcggctacc gcgatcgcgc aggcgagccg tacgccctcg
     2461 tcgagatcgc actcgagcgc aagctcgtga tgatcatccg cgagcacaag ctcgtctccg
     2521 ctccacgtcc agcgccgccc acaccgatgc accgtcgccc atcgcgtcgc gcgggctctc
     2581 gccattcgcg ctagctcgcg tctgagcgcc ggcgtgatcg ctggctaaat gccgagctcg
     2641 gcgagtgctt gctgcagacg acgcacggag acgggcttcg tcagatgcgc cgagaatcca
     2701 gcctcgcgtc cgcgggcttc cgcgtcatcg ccggacgtgc cggtaatcgc gatgcccttc
     2761 atcttgccgc ggagcttcgt catgagctcg tagccgcttg catcgggcaa cccgacgtcg
     2821 ctcacgacca catcgaacgt gttcgactcc gcgaggctca gcgcatcggt cgcgcaccgc
     2881 gcgatgctca cggcatagcc gttgttctcg agcagctgcg cgagcaaatc agccgaatcc
     2941 tcgtgatcct cgacgagcag gagccgaaca gccataacga gcgcagtcaa ccatgccgat
     3001 ggtcaccctt caatgggaat tggtgctcct gacttacgaa tgtcttacgt cgcggcctgt
     3061 cgcgttcggc catcgtcagc acacggagga accgacgatg aaaatgcttg ctttgctgac
     3121 gacgatcttc gcaaccgcga ccggatgcca caccacctcg ggcccgacgg ccgccgcgcc
     3181 gtccagtccc gcgtccctca cccagccaac ggccgatctc acgcagccaa cggccgacct
     3241 tgccggccgg cgcgcccaga tgctcgccta tctccacgac tacaccgaag ccggtcaata
     3301 cccgaccgac gatgccggcc tgccccgaag cgtgttcgtc gacgcgaaag gcgtccgctg
     3361 cccgatggcc gagctcattt tccgctccgg ccacgccgac ctggtggacg cggtcgcccg
     3421 cgagcacaac gaagtccgcc tctccgacgt gaatgaaggc ccgctcctgg cgtggatgca
     3481 gagctccgga ctgacgcgcg acgagatcaa cctcgtccaa ggtgtcgccg acctcgacat
     3541 ggtctggatc caagaggaag cgccgagcca ggagacgatc ctcgcccgtg gccagatccg
     3601 cggccgcctc gaatccgccg agcgcgcgct ccgcgacggg acgacgcact cgctccaagt
     3661 cgcggtcgcc gcgctgccaa acggcaagct gcccgaagcg aagctcgccg ccggcgcgaa
     3721 ggtcgtcccg gtcgccgcga tgaagtcggg gccccaaggc cgcccgctgc gcgcagtcgc
     3781 gctggctccg cgctggcgga cgtcgccgca gatggtggtc gctccgaacg gctcgatcac
     3841 gctcgcgccg gcggtcagaa actaagacca cgtcgacgac gtcgcggtcg acgcggtcgt
     3901 cgaggccgac attttccgca tcgcggtcgt gacgagctcg acgggaatcc gcccacttcg
     3961 catcggctcc gcgacagtgg accgcggctt gtcgtccttg ctcgtcgcga ccgcgatgac
     4021 ggtccctatg tagaggcccg ctgcctcgaa tggcagcacc cagaatgtga ccttcaccgc
     4081 gatgatgaag ccgacggtcg cgccggccca ctggacgatc gaccacatcg cggctcggtg
     4141 aggcgcgcgg cacgagatcg cgacgatgca gcgcgcatgc gcgaagtcga tgagggtggc
     4201 agagacgaag acaacgaagc agaggagcgg cgtcatgcag acatccttgc tgacgtaggc
     4261 gtgaagccac ccgacgccga tcgtgaagct cgcgaccgag ccgcgtgaag ctcgcggccg
     4321 aggcgcgtga agcgagcgtg atcgcagcga cgggcatcgc gagctcgcac ggttccacac
     4381 gactcgtacc cacgtgggca cagcgtatgc tgtccgcgtg atcggcctcg ggtgcatgcg
     4441 gctgtcgacg gacgcgagtc gcgatgatgc gcgttcgctc gcggtcctgc gcgttgcgct
     4501 cgacgaaggc atcacgctgt tcgacacggc ggacgcctac gcgctcgacg atctcgacat
     4561 cgggcacaac gagaagctcc tcgcgcgcgc gctcgccgag gaacttcgcg cccacgtggg
     4621 cacatcgcgt gtcggtacgg acggtcgtgc ccacgtgggc acatcgctcg tcgctacgga
     4681 cggtcgtgcc cacgtgggca catcgctcgt cgctacggag ggccgtgctc acgggggccc
     4741 accggtagtc gttacgaagg gccgtgctca cgggggcaca ccggtcgtcg ttacgaaggg
     4801 cgggctcgtg cggcccgacg gcgcgtgggt gccgaaaggc aacgcgcggc atctcggtct
     4861 agcagcgcgt tcgagtcgcg agcgtctggc ctcgcgtgat gcgaccggcg cggagggcgc
     4921 gagcggcgcg accagcggca acgagtccgc cgcgccgatc gatctgtact tgctgcacgt
     4981 cgtcgatcct cgcgtgcctc ttgcgacgag cgtgcgcgcg ctcgcgaagc tgcgcgacga
     5041 cggcatcgtg cgcgcgatcg gcttgtcgaa tgtcacgcag catcagctcg agcaagcact
     5101 cgcgctcacg cagatcgagg cgatcgaggt cgagctcggg ccgcacaagc tcgacgcgtt
     5161 tcactcgggg ctcgtcgact tttgccggca gcgtgacatt cgtgtgctcg cacatcgtcc
     5221 gctcggcggg ccgaatggcg tgaagcgtct cgcgcgcgac aagcgactcg cgcaactgtg
     5281 cgagaacgcg aattggtcag accggcctag cgatgcgccc gagcattcac cggatgccga
     5341 gcattcagcg agtgcgtcgg ttcaatggcc gacgattcac gagctcgtgc tcgcgtggct
     5401 cgctgcgaag gacgtcgtgc cgattcccgg cgcgacacgc gtcgagacgg tgcgaagtat
     5461 cgcccgcgcg aatcggttgc agctctccga ggagctcgtg cgcgagctcg acgcgcactt
     5521 gctcgacgag ccctcaacga cacgt
//
LOCUS       DATLBZ010000439         4816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7724361, whole genome shotgun sequence.
ACCESSION   DATLBZ010000439 DATLBZ010000000
VERSION     DATLBZ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7724361"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..984)
                     /locus_tag="VMZ53_22235"
     CDS             complement(97..984)
                     /locus_tag="VMZ53_22235"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS31253.1"
                     /translation="MPGAATVRYGGQTSCVEARTANGETLIVDAGTGMRQLGNKLARE
                     AAGAPGQYHVLLSHVHWDHIQGLPFFSPAYIPGTKISVYALLTAADELNQVIGGITRH
                     EFFPMPLEAVPANFDFHQVEPGVDFVVGRFHVIPIALNHPFGSVGYRIDADGSSWAYV
                     SDTAPFDKVLHKQHFLAGPEPLSPDDLTALGAMREALIKRLQGVDTVVYDTHFLPEEY
                     ARFPHYGHSTPDQALVICKEAGVRRLVLYHHAPSHTDEQMDKIAAEYLAKGASIGIEV
                     LTSFEGMTLPIGSQAGEQK"
     gene            1096..2142
                     /locus_tag="VMZ53_22240"
     CDS             1096..2142
                     /locus_tag="VMZ53_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HUS31254.1"
                     /translation="MNPQPRILVMGAGGIGGIVSATLTEVGSAVTAVSTNDSIRAAVD
                     KSGYRIVDEGEERTVRGWVSPAPEGKYDLCILATQPPNVEDAARTALPHLADDAEVVV
                     LQNGLCEDRIGAIVGPERVIGAIVAWGASMPEPGRYERTASGGFTIGRMSGVIDKDVE
                     RVAELLEAIGPVTLTQNLAGARWSKLALNCAVSALGTIAGDRLGPLVRVRRYRRLGLE
                     AMTECVQVAKAEGIKLEKVAGTLDLDWIALSQADRESSGSVALTAKHALLLAVGLRYR
                     RMRSSMLAAIERGRTPAIDFLNGEVVTRGAKHGIATPFNARIVETVWSIAKGSSKSSR
                     ETLDKVAERISDEK"
     gene            2216..2641
                     /locus_tag="VMZ53_22245"
     CDS             2216..2641
                     /locus_tag="VMZ53_22245"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS31255.1"
                     /translation="MSGGRRDPRIKTNTRALVWFGSRAAQFTIDNLSLGGARLIGSMA
                     LRLGQQVDIELDLDTGPIKVTGEVVRVDTPDLLEDQIAVRFVNTAPEVKTAIRDVVAR
                     QLAAAEDDDAAGDEETTRGDPVVGKLGDDEPEIELETDD"
     gene            complement(2661..3230)
                     /locus_tag="VMZ53_22250"
     CDS             complement(2661..3230)
                     /locus_tag="VMZ53_22250"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS31256.1"
                     /translation="MTDASVEQIVRVPVLETERLVLRGPRLADFADSFGMWRLPEVNK
                     HIGGKPRTEEDSWSRLQRYVGHWALLGFGMWMVREKSSGAYVGEVGFMDNRREMTPAF
                     TAVEVGWVLMPQHHGKGYATEAMRAALAWGDDHFTRAPLDERLVRGRVECIIDDGNDA
                     SVRVATKLGFVRLGTADFKGEPVVLYRRG"
     gene            complement(3262..>4816)
                     /locus_tag="VMZ53_22255"
     CDS             complement(3262..>4816)
                     /locus_tag="VMZ53_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31257.1"
                     /translation="GRADVAGGRADVGAARKDAAAIETRSRARLAGAIACGVAIASVQ
                     WLPAIFAAPHIVGSSIEGIRVARFLELFVPMRSPDIHVPMIFVGAPLLALAAVTRPDR
                     RMIAFAAVLFVASAFWGQHLAVLALLAAAHAGTAIDALFAADNEVLAQRRRALIALAA
                     AALLTAIAVTALGTLRSRIDDTAQRALLDHTVRDGIIAVACAAGAALLAWRVRVNITP
                     LVVALLLVAPGVLAQHSMLPVMDRDVVDEPGPWAARALTSSPPVRVYRPIKLLEDVHD
                     TSAPTLDAELATFSGASGSLWGVDAARSEDPARITTHDRTWFAASSAGGLLLERYGIS
                     LAILPLSSIGQREGTTVELGRRGSWALVRFPASPPASVVREWIFAPDDDTALHRLFPP
                     GSRHGLPTGLIVLHGTGRENQDEESPPTPCTIERWNAASIDLVCTASTESPEAYAVVS
                     STPMPGWSVTVDDRATPWLTADVLRRAVAIPAGTHRVQWRYHAPGLTPALILAALGIL
                     GLVGLRVKR"
BASE COUNT          848 a         1572 c         1663 g          733 t
ORIGIN      
        1 ccgtcacgat ctcgatcgaa gtggtgttgc cgccgtagcg aaggaactcg cgtcccgaca
       61 tcgcgaacga gccgcgaaca ccccagaagc gtactttcac ttctgctccc cagcttgcga
      121 accaatcggc agcgtcatgc cctcgaacga cgtcagcacc tcgatgccga tcgatgcgcc
      181 cttcgcgagg tactccgcgg cgatcttgtc catctgctcg tcggtgtgcg acggcgcgtg
      241 gtgataaagg actaagcggc gcacgcctgc ttctttgcag atcacgagcg cctgatccgg
      301 cgtcgagtga ccatagtgcg ggaaccgcgc gtactcctcc gggaggaagt gcgtgtcgta
      361 gacgacggtg tcgacgccct gcaggcgctt gatgagtgcc tcgcgcatcg cgccgagcgc
      421 ggtgagatcg tcgggcgaga gcggctcggg gccggcgaga aagtgctgct tgtggagcac
      481 cttgtcgaat ggcgcggtgt cgctgacgta cgcccacgac gagccatccg catcgatgcg
      541 gtagccgacc gacccgaacg gatggttcag cgcgatcgga atgacgtgaa agcgtccgac
      601 gacgaagtcg acaccgggct cgacctgatg aaagtcgaag ttcgcgggca ccgcctcgag
      661 cggcatcggg aagaactcgt gacgcgtgat gccgccgatg acttggttca gctcgtcggc
      721 tgcagtcagc agcgcgtaga cggagatctt cgtcccgggg atatacgcgg gcgagaagaa
      781 cggcagacct tggatgtgat cccagtgaac gtgcgagagc agcacgtggt actggcccgg
      841 cgcacccgcg gcttcacgcg cgagcttgtt gccgagctgg cgcatgccgg tgcccgcgtc
      901 gacgatcagc gtctcgccgt ttgcagtgcg cgcttcgacg catgacgtct gcccgccgta
      961 tcgcacggtg gctgcgccag gcacggggta cgagccccgg accccccaaa acgtaatctg
     1021 catgcggtgc gataattcta cgccgtcctc gggattcccc aaagttccag cacttttcgt
     1081 ataaaggacc acagcatgaa cccccagccg cgaatcctcg tcatgggcgc cggcggtatc
     1141 ggaggcatcg tttctgcgac cctcacggag gttggatcgg ccgtgacggc ggtctcgacg
     1201 aacgattcga tccgcgccgc cgtcgacaaa tcgggctatc gcatcgtcga cgaaggcgag
     1261 gagcgcaccg tccgcggctg ggtgtcgccc gcgcccgagg gaaaatacga cctctgcatc
     1321 ctggcgaccc agccacccaa cgtcgaggac gccgctcgca ccgccctgcc ccatctcgcg
     1381 gacgatgccg aagtcgtcgt cttacagaac ggcttatgtg aggaccggat cggcgccatc
     1441 gtcggacccg agcgggtgat tggcgcgatc gtcgcgtggg gcgcgtccat gccggaaccg
     1501 ggccgctacg aacgcacggc gagcggcggc ttcacgatcg gccgtatgtc gggcgtgatc
     1561 gacaaagacg tcgagcgcgt cgccgagctg ctcgaagcga tcggcccggt cacgctcacg
     1621 cagaacctcg ccggcgcgcg atggagcaag ctcgcactca actgcgccgt gtccgcgctc
     1681 ggcacgatcg caggcgaccg cctcggcccg ctcgttcgcg tgcgtcgcta tcgccggctc
     1741 ggtctcgaag cgatgacgga gtgcgtgcag gtcgccaagg ccgaaggcat caagctcgag
     1801 aaagtcgccg gcacgctcga cctcgattgg atcgcgctgt cgcaagcgga tcgcgaatcg
     1861 tcgggctcgg tcgcgctcac cgcgaagcac gcgctcctcc tcgccgtcgg cctccgttac
     1921 cgcagaatgc gctcgtcgat gctcgcagcg atcgagcgag gccgaacccc ggcgatcgat
     1981 ttcctcaacg gcgaggtcgt gacccgcggc gccaaacacg gcatcgcgac gccgttcaac
     2041 gcgcggatcg tggaaacagt gtggtcgatc gctaagggat cctcgaagtc gtctcgcgaa
     2101 accctcgata aggttgccga gcggatttcc gacgagaaat aggcctgccg gatcaggcag
     2161 tgactgccag acatccgaat tcgtcaaatg ggtccgacca tgctgtagta ctcgggtgtc
     2221 ggggggccgt cgagatccgc gtatcaagac gaatacgcga gcgctggttt ggttcggctc
     2281 gcgtgccgcg caattcacga tcgacaacct gtcgctcggt ggcgcgcgtc tgatcggatc
     2341 gatggcgctg cgcctcggcc agcaggtcga catcgagctc gatctcgaca cgggtccgat
     2401 caaggtcacg ggcgaagtcg ttcgcgtcga cacccccgac ctcctcgaag atcagatcgc
     2461 cgtccgcttc gtcaacacag cgcccgaggt gaagaccgcg atccgcgatg tcgtcgcgcg
     2521 ccagcttgct gcggccgaag acgacgacgc cgccggcgac gaagagacga cgcgcggtga
     2581 cccggtcgtc ggcaagctcg gcgatgacga gcccgaaatc gagctcgaga ccgacgacta
     2641 gcgcccgcgc ttccgcgcgt tcagccgcgg cgatagagga cgacgggctc gcctttgaag
     2701 tcggcggtgc cgaggcgtac gaagccgagc ttcgtggcga cgcggacgga agcgtcgttg
     2761 ccatcgtcga tgatgcattc gacgcggccg cggacgagac gctcgtcgag cggtgcgcgc
     2821 gtgaagtggt cgtcgcccca tgcgagcgcg gcgcgcatcg cttcggttgc gtaacctttg
     2881 ccgtggtgct gcggcatcag cacccagccc acttcgaccg cggtgaatgc gggcgtcatc
     2941 tcgcggcggt tgtccatgaa gccgacctcg ccgacatacg cgccgctcga cttctcgcgc
     3001 accatccaca tgccgaagcc gagcagcgcc cagtgaccga cgtagcgctg cagccgcgac
     3061 cacgagtctt cttcggtgcg cggcttgccg ccgatgtgct tgttgacctc gggcagtcgc
     3121 cacatgccga acgaatcggc gaagtcggcg agcctcggcc cgcgtagaac gagccgctcg
     3181 gtctcgagca cgggaacgcg gacaatctgc tcgacggacg cgtcggtcat cgcagcacac
     3241 gcggtgtatc gagtttcgcg gtcatcgctt cacgcgcaag cctaccaggc cgaggatgcc
     3301 gagcgcggcg aggatgagcg cgggcgtgag tcccggcgcg tgatagcgcc actgcacgcg
     3361 atgcgtgccc gccggaatcg caacggcgcg gcgcaggaca tcggcggtga gccacggcgt
     3421 cgcacgatca tcgacggtga cggaccaccc cggcatcggc gtcgacgaca cgactgcgta
     3481 ggcctctgga gattccgtcg aagcagtgca cacgaggtcg atcgatgctg cattccagcg
     3541 ctcgatcgtg cacggcgtcg gtggactctc ctcgtcttga ttctcgcggc ccgtgccgtg
     3601 aagaacgatg aggccggtgg gcagtccgtg gcgcgagccc ggcggaaaga ggcggtgcag
     3661 cgcggtgtcg tcgtcgggcg cgaagatcca ctcgcggacg acagatgccg gcggcgatgc
     3721 ggggaagcgg acgagcgccc agctaccgcg gcgaccgagc tcgacggtcg tgccttcgcg
     3781 ctggccgatc gacgagagcg gcaagatcgc gagcgagatg ccgtagcgct cgagaagcag
     3841 gccgcccgcg gacgatgccg cgaaccacgt gcggtcgtgc gtggtgatgc gtgcgggatc
     3901 ttcgctacgc gcggcatcga cgccccacag cgagccggag gcgcccgaga atgtcgcgag
     3961 ctcggcgtcg agtgtcggcg cggacgtgtc gtgcacatct tcgagcagct tgatcgggcg
     4021 atacacgcgg acgggcggcg aggaggtcaa cgctctggct gcccacgggc cgggctcgtc
     4081 cacgacgtcg cggtccatga ccggcagcat cgagtgttgg gcgaggacac ccggcgcgac
     4141 gaggaggagc gcgacgacga gcggcgtgat gttcacgcgc acgcgccatg cgagcagcgc
     4201 tgcgcctgcc gcgcacgcga ccgcgatgat gccgtcgcgg accgtgtgat cgaggagtgc
     4261 gcgttgcgcg gtgtcgtcga tgcgcgaccg cagcgtgccg agcgcggtga ccgcgatcgc
     4321 ggtgagcagc gccgcggcgg cgagtgcgat gagtgcgcgg cggcgttgtg cgagcacttc
     4381 gttgtcggcg gcgaacagcg cgtcgatcgc ggtgccggcg tgagcggcgg cgaggagcgc
     4441 gagcactgcg aggtgctggc cccagaatgc gctcgcgacg aacaggaccg ccgcgaatgc
     4501 gatcatgcgg cgatcggggc gcgtgaccgc ggcgagcgcg aggagcggcg cgccgacgaa
     4561 gatcatcgga acgtgaatgt ccggcgagcg catcgggacg aacagctcga gaaagcgagc
     4621 gacccgaatg ccttcgatcg aagaaccaac gatgtgcggc gccgcgaaga tcgcaggcag
     4681 ccactgcacg gaggcaatcg caacgccgca cgcgatcgca ccggcaagtc gcgcacgcga
     4741 acgcgtctcg atcgcggcgg catccttgcg cgccgcgccg acatccgcgc gcccaccagc
     4801 gacatccgcg cgccca
//
LOCUS       DATLBZ010000440        11418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9493722, whole genome shotgun sequence.
ACCESSION   DATLBZ010000440 DATLBZ010000000
VERSION     DATLBZ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11418
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9493722"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2080
                     /locus_tag="VMZ53_22260"
     CDS             <1..2080
                     /locus_tag="VMZ53_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31258.1"
                     /translation="INLVGPDHKEQCDGGTTGSPKETTLCNLDCTNAVCGDNKINQTA
                     GEQCDSTSGVDSPTCNGQSGGAVKCKLTSCGDNFINGAAGETCDATSGSDSPSCNGSS
                     AGQKACKAAVCGDNFLNSQAGETCDSSNGADTAGCNGALAGVGKACKVSVCGDNFVNP
                     AANEQCDSSGGNDVAGCNGFGAGAQSCRFSSCGDNYLNPQDGEQCDASNGNNTPTCNG
                     IAAGVKKCLFSACGDNFINPAAGETCDATNGADTAGCVGNSAQALTLGIACKASTCGD
                     SYANPAALEDCDAAGADTSACNGVNAGTKKCKFATCGDNYVNPQASETCDSSSGVNTA
                     TCNGNLATPTSVRCKAASCGDTYRNSAAGEDCDTGPADSATCNGNSAGAVKCKTPACG
                     DSHPNTQAGENCDDGNNATELCAYGAPSCSVCRAGCTTGGGTTQSCNDGVVQSAFETC
                     DDGNASCGTCSTNCQVFASAQAKGSISVVRGNVIASGDVFRLDDGYNPPITFEYRLSG
                     AAATGNMSIPVGSGDNLGSVRGKTRDAINYSVNIETTGNAGASETGTTATFTTTSNHR
                     FTTGLTVVVSGVGVAGYNGKWVITGTPAADKFTVTLPTSGLAASGGGSVSLALDITAT
                     SGDTFSIVVLTNDHFSLNGNTAITFTASDPDFSKVDMQGGAGGDCDTGLVCRSNPDCD
                     NGLCSSNACN"
     gene            2147..2914
                     /locus_tag="VMZ53_22265"
     CDS             2147..2914
                     /locus_tag="VMZ53_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS31259.1"
                     /translation="MNLSQLKIVVTGGAQGMGAHFARRLAEAGAQVAVGDVKEEGLAQ
                     LAESARGLPGKIHTRKLDVSSEADVTGFVDWAYGAMGGLNGLINNAGILRDGLLVKKD
                     KTTGEITKMSKDQFEAVINVNLTGATMMVREVVAKMVSAEQKPGVVVNMSSIARHGNR
                     GQSNYVSAKAALAANTTTWAREFAAFGIRVCGVAPGMVETPMTQGMNQKARDAIVAAI
                     PVGRIGTPEDLWLAVKFCLECDYFNARTFDVDGGMGF"
     gene            complement(2922..4346)
                     /locus_tag="VMZ53_22270"
     CDS             complement(2922..4346)
                     /locus_tag="VMZ53_22270"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HUS31260.1"
                     /translation="MVATGFLYEHLADELNQAIDRGALRAGDRLPSVRRLAQERSVSV
                     STVLEAYLQLENAGLIEVRPKSGHFVRRRAALTAEPRMPRVCQRPSRVSATDAYTKIL
                     EAMRDPELVPLGCATIDPSYLPMAQLNRTVAQMTREMTTLGARYEAAPGLITLRRQIA
                     RRAVESGAALSEHDLCTTVGATEGITLALRAIAKPGDVIAVESPAYFGVLQAIEKLGL
                     RALEIPANPRNGLDVDAFEEAIRGQPIRALFVSPTISNPLGSIMPDDERERLVRIAKR
                     ADIALIEDDVYGELVFDGSRPRTLRSFAGPSEDNNVILISSVSKTLAPGYRVGWIAGG
                     KYHDQIVRMKLSQSLADPPLLGMAVAEFLSSGGYDRHLRRLRTAVSGNVERYREAIAN
                     DFPDGTRVAAPRGGFVLWVELPPGADALELHEQALRKGIVVAPGPIFSARQRFTNFVR
                     ISAGTPWSERIASAIRTLGRIARV"
     gene            4478..5410
                     /locus_tag="VMZ53_22275"
     CDS             4478..5410
                     /locus_tag="VMZ53_22275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HUS31261.1"
                     /translation="MRPVVTTTSSPEAVAPVAPKIDPRLIASLAAVYVIWSSTYLAMR
                     IVVHDLPPLLSAALRFLLAGGTMFGYALYKGARFPSLRDWLRVAPVGIFLFVGGNGFV
                     SIASQSIPSGGIAVVCALMPLWAGVLGFAFGVRPTRREWFSLFIGFAGVVILLGGPSL
                     AGKPIHIALVIASPMLWAAGSLLSRRTKDVGGVHAPLIGPALQMLTGGAALAAVALVR
                     GERFPVDASATSWAALVYLFIFGSLVAFTAYAWLLRNTRPAIATSYAYINPILAVLIG
                     AALYGEPLGVTTLIANILIVGAVMLALSGTRHRV"
     gene            5424..6620
                     /locus_tag="VMZ53_22280"
     CDS             5424..6620
                     /locus_tag="VMZ53_22280"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31262.1"
                     /translation="MLRVVLRLLLASAVVTTLPACPGSTQTKTSNKKTKKAKKADTKA
                     LVSEARDDAKNGDYDAADAKYAEAWETSKDFDVLEERVDFLIHAGRATKAAEAAKAFL
                     DANISDKNAYILYAEALLAGNKGAEALEVADQLIQLNADEPAGYEKKGRALILLEKNE
                     EGIDMLRKAVQMDNTSASFHRSLGIALNKLGKIDESALEFRAAIKADSDDAEAHVLLG
                     MALRGQQEYDEAKSYLDKAIELDPRNGRAYFEMGLLYNQQKKQDLAEAALSKAVQLSP
                     NESLFWYAYGEIYRLQERFEDAITAYKKATDIDPPYTKAMFKLGSLLIQKKSYDEAEV
                     VLTNAVRREPKNPSGYLVLGDLYAAKRKAKLGIESYQKYLELAPKSDPERDRAQSAIR
                     ELKRQR"
     gene            6682..7332
                     /locus_tag="VMZ53_22285"
     CDS             6682..7332
                     /locus_tag="VMZ53_22285"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HUS31263.1"
                     /translation="MTDVGIDPPAAILRWAARHIPRLAFATGFGAEGCVIIDLIAREQ
                     LPIDIFTLDTGVLFPETYALWKALEAKYGITIRGVAPVQAIDKLWETDSDRCCDLRKV
                     QPLRAALAGFGGWITAIRRDQTPERANAAVVEADPKFGLIKVNPLVAWTHDDVWAHLY
                     ANDVPTNALHQQGYPSIGCQPCTSPIVPGENLRAGRWRGAQKTECGLHTPLKEAAK"
     gene            7329..7898
                     /locus_tag="VMZ53_22290"
     CDS             7329..7898
                     /locus_tag="VMZ53_22290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HUS31264.1"
                     /translation="MSDARLYPVFLRLEGLPVVVVGGGPVAASKLDGLLAAGARVTVI
                     APHVLPEIRAKATVVEQPFRAAHLAGARWVIAAATPDVNRDVAAAAAARGLFVNAVDD
                     TRVATAYLGGVVRRGPVEIAISTGGLAPALAGLLREALDALLPEDLDKWIEVANEARR
                     DWKQRKVPMPERRPLLLRALDRIYAGASS"
     gene            7895..8641
                     /gene="cobA"
                     /locus_tag="VMZ53_22295"
     CDS             7895..8641
                     /gene="cobA"
                     /locus_tag="VMZ53_22295"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR01469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HUS31265.1"
                     /translation="MSGFVSLVGAGPGDRELLTLRAADRLRNADLVLYDALTPEDILE
                     LAPQAKRFYVGKRAGRHSIDQDGIHRLMVRAANRGERVVRLKCGDPFVLGRGGEEALA
                     LEEAGIAYEVVPGLSSAIAAPALAGIPVTHRGLSQGFAVFSGHTTQHADSIAPHTLTL
                     VVLMGLRTRAELAERLIARGWSPATPAAIVLGASHEGSTRWLGTLETLATADIDGDLA
                     GIIVVGAVVELATQIANDYTSRTKLAARIS"
     gene            8638..10377
                     /locus_tag="VMZ53_22300"
     CDS             8638..10377
                     /locus_tag="VMZ53_22300"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HUS31266.1"
                     /translation="MSVTEAPAPVPVGRLGFARHEDVDLFVQRLEAFENGELSPDEWR
                     SFRLLNGVYGQRQDGVMMVRAKLPAGIATPAQLLALADVAEKFSAGERGHVTTRQNVQ
                     FHFVPQDKTEEALRILADSGITTKEACGHSVRNWTCCPLAGVAKTEAFDPTPYVEALA
                     RHLLRGTYSSTLPRKLKPSLGGCCGTDCSQAFINDLGFLATSRDGAHGFKLLAGGGLS
                     TLRREAIVVEEFVPVAEVLEAADAIVRVFHRIGNRNNKAKARLKWAIEKIGVDAFLAE
                     YRAERETIRSEGGVPLVLPAQPVPPSRRPLLAQVSEPADGYSEWAADSVRAQKQAGFS
                     AVVIRLILGDITATQLRALAQIAKTYGEGEVRLTNEQNIVLRWVPDARLPALHRELAA
                     IGLANIGANTLADVTSCPGATSCKLAVTSSKGLAAVLTQFLDERQDLVRKAKGLDIKV
                     SGCPHGCGQHYIGGIGFQGGMRKLAGRAAPLYLVYVGGGITATGADFGKLIGKVPARR
                     VTTLLERLLGFYIAAGGTSHKFWNDVPVDQVRELIADLTVIDDATATEEDFIDHGEAA
                     AFEVITAEGECSA"
     gene            complement(10385..10678)
                     /locus_tag="VMZ53_22305"
     CDS             complement(10385..10678)
                     /locus_tag="VMZ53_22305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31267.1"
                     /translation="MRAVALLAILALIPGCFGYNPGAKKWSYAGNSVLIAGGGGAIAG
                     DILSKDEPCPTTSMNGCYQPKFGGGMVAGVMLVTAGIIGIVINATRPNVKTSR"
     gene            10782..>11418
                     /locus_tag="VMZ53_22310"
     CDS             10782..>11418
                     /locus_tag="VMZ53_22310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS31268.1"
                     /translation="MIPLLAFYAEKFGASPLVATTLVSVYAVCSLISTPIIGNLSDRY
                     GRRRLLLVSQAGTCLGFLMLAWSHTLWMVFLGRILDGITAGNLSLAQAYISDHTKPEN
                     RAKAFGVIGVAFGIGFMFGPGIGGLLGKYGLHVPFLVAASLSLLSIIATATLLKNEVP
                     GAPTEADEATPATEGQPPAGRRPGAFDIATYTEYFRRPKLGNLYLQFFLFTF"
BASE COUNT         2000 a         3813 c         3722 g         1883 t
ORIGIN      
        1 gatcaacttg gtcggccccg accacaaaga gcaatgcgac ggcggcacga cgggctctcc
       61 gaaagagacg acgctctgca acctcgactg cacgaacgcg gtctgcggcg acaacaagat
      121 caaccagacc gcgggcgagc agtgcgactc gacgagcggc gtcgactcgc ccacgtgcaa
      181 tggtcagagc ggcggcgcgg tgaagtgcaa gctgacctca tgcggcgata acttcatcaa
      241 cggcgcggcc ggcgagacgt gtgacgccac cagcggctcc gactcgccgt cgtgcaacgg
      301 ttcgagcgca ggccagaagg cctgcaaggc cgccgtctgc ggtgacaact tcctcaactc
      361 gcaagcgggc gagacgtgcg actcgagcaa cggcgccgac acggcaggct gtaacggcgc
      421 tttggccggc gtcggcaagg catgcaaggt ctcggtgtgt ggtgacaact tcgtcaaccc
      481 cgcagcgaac gagcagtgcg actccagcgg cggcaatgac gtggctggtt gcaacggctt
      541 cggcgccgga gctcagtcgt gcaggttctc gtcgtgcggc gacaattatc tcaacccgca
      601 ggacggcgag cagtgcgacg cgagtaacgg caacaacacg ccgacgtgca acggcatcgc
      661 cgcaggcgtg aagaagtgtc tgttctcggc gtgcggcgac aacttcatca atcccgctgc
      721 cggcgagacc tgcgatgcga ccaacggcgc cgacaccgcc ggttgcgttg gcaacagcgc
      781 gcaggcgttg acgctcggga tcgcctgtaa ggcgtccacg tgtggcgata gctacgcaaa
      841 cccagccgca ctcgaagatt gcgacgcggc cggtgcggac acgagcgcgt gtaacggcgt
      901 caacgcgggc acgaagaagt gcaagttcgc gacgtgcggc gacaactacg tcaacccgca
      961 ggccagtgag acctgcgatt cgagcagcgg cgtgaacacg gccacctgta acggcaactt
     1021 ggcaacgccg accagcgttc gctgcaaagc tgcgtcgtgc ggtgacacgt atcggaacag
     1081 cgctgcgggc gaagactgtg acaccgggcc agcggactcg gcgacgtgta acggcaatag
     1141 cgccggcgcg gtgaagtgca agacgccggc ctgcggcgac agccacccga acacgcaggc
     1201 gggcgagaac tgcgacgacg gcaacaacgc gacggagctc tgcgcgtacg gtgctccttc
     1261 gtgcagcgtg tgccgtgcgg gctgcacgac aggcggaggt acgacgcaat cgtgcaatga
     1321 cggcgtcgtc cagtcggcgt tcgaaacgtg tgacgacggc aacgcgtcgt gcggtacgtg
     1381 cagcacgaac tgccaggtgt tcgcgtccgc gcaggcgaag ggctcgatct ctgtcgtgcg
     1441 cggcaacgtg atcgccagcg gcgacgtgtt ccggctcgac gacggctaca acccgcctat
     1501 cacgttcgag taccggctca gtggtgctgc ggccaccggc aacatgtcga tccccgtcgg
     1561 cagcggcgac aacttgggca gcgtgcgcgg caagacgcgt gatgcgatta actactccgt
     1621 caacatcgag accacaggta acgcgggtgc gagcgagacc gggacgaccg ccacgttcac
     1681 gacgacgagc aaccatcgct tcacgaccgg cctcaccgtg gtcgtctcgg gcgttggcgt
     1741 ggcgggatac aacggcaagt gggtcatcac cggtacgccc gcggcggata agttcaccgt
     1801 gacgcttccg acgagtggtc tcgcggcatc gggcggcggc agcgtgtcgc tcgcgctcga
     1861 catcaccgcg accagtggcg acacgttctc gatcgtcgtt ctgacgaacg atcacttctc
     1921 gctcaacggc aataccgcga tcaccttcac cgcaagcgac cccgacttct cgaaggtcga
     1981 catgcagggc ggcgccggcg gcgattgcga taccggtctc gtgtgccggt cgaacccgga
     2041 ctgcgacaac gggctatgta gcagtaacgc ctgcaactag cgtcggtcgc aacggaactt
     2101 tgctgcgctg caacattttc ggcgtgggtg tgttaaacac ccgcgcatga acctctcgca
     2161 gctgaagatc gttgtcacgg gtggcgccca gggcatgggt gcgcacttcg ctcgccggct
     2221 tgccgaagct ggtgctcaag tcgccgttgg cgacgtcaag gaagaggggc tcgcgcagct
     2281 tgccgagtcc gcgcgcggtc tccccggcaa gatccacacg cgcaagctcg atgtgtcgag
     2341 cgaagcggat gtgacggggt ttgtcgattg ggcgtacggc gcgatgggcg gcctcaacgg
     2401 cctcatcaac aacgccggca tcctgcgcga cggtctcctc gtcaagaagg acaagacgac
     2461 cggcgagatc acgaagatga gcaaggacca gttcgaagcg gtcatcaacg tcaacctgac
     2521 aggcgcgacg atgatggtgc gcgaggtcgt tgcgaagatg gtctcggccg agcagaagcc
     2581 gggcgtcgtc gtcaacatgt cgtcgatcgc gcgtcacggt aaccgcggcc agtcgaacta
     2641 cgtgtcggcg aaggctgcgc tcgccgcgaa caccacgacg tgggcgcgcg agttcgccgc
     2701 gttcggcatt cgcgtgtgcg gcgttgcgcc gggcatggtc gagacgccga tgacgcaggg
     2761 catgaaccag aaggcgcgcg atgcgatcgt cgccgcgatt ccggttggcc gcatcggcac
     2821 gccggaagat ctctggctcg cggtgaagtt ctgcctcgag tgcgactact tcaacgcgcg
     2881 cacgttcgac gtcgacggcg gcatgggctt ctagtcggcg cctacactcg agcgatgcgg
     2941 ccgagcgttc gaatcgcgct cgcgatccgt tcgctccacg gcgttcccgc cgagatcctg
     3001 acgaagttcg tgaagcgctg gcgcgccgag aagatcggtc ccggcgcgac gacgatgcct
     3061 ttgcgcagcg cctgctcgtg cagctcgagg gcatcggcac caggcggcag ctcgacccac
     3121 agcacgaagc caccgcgagg agccgcgacg cgcgtgccgt ccgggaagtc gttcgcgatc
     3181 gcctctcggt agcgctcgac gttgccggag actgcagtgc gcagccgccg caagtgacga
     3241 tcgtagcctc cgctcgacag gaactcagcg acggccatcc cgaggagcgg cggatcggcg
     3301 agtgactgcg agagcttcat gcgcacgatc tgatcgtgat acttgccgcc cgcgatccag
     3361 ccaacgcggt agccgggcgc gagcgtcttg ctcaccgagc tgatgaggat gacgttgttg
     3421 tcctcgctcg gtccggcaaa cgagcgcagc gtccgcgggc gagagccgtc gaacacgagc
     3481 tcgccgtaca cgtcatcctc gatgagcgcg atgtcggcgc gcttcgcgat gcgcacgaga
     3541 cgctcgcgtt cgtcgtccgg catgatcgag ccgagcggat tgctgatcgt cggcgacacg
     3601 aacagcgcgc ggatcggctg gccgcgaatc gcctcttcga acgcatcgac gtcgaggcca
     3661 ttgcgcgggt tcgctggaat ctcgagagcg cgcaggccga gcttctcgat cgcctgcagc
     3721 acgccgaagt acgccggtga ttcgaccgcg atcacgtcgc ctggtttcgc gatagcgcgc
     3781 agcgcgagtg tgatgccttc ggtcgccccg accgtcgtgc acagatcatg ctccgacagc
     3841 gcggcgcccg attcgacggc gcgccgagcg atctgccggc gcagcgtgat caagcccggt
     3901 gctgcttcgt agcgcgcacc gagcgtcgtc atctcgcgcg tcatctgcgc gacggtgcgg
     3961 ttgagctgcg ccatcggtag atacgacgga tcgatcgtgg cgcagccaag cggcacgagc
     4021 tcgggatcgc gcatcgcctc gaggatcttc gtgtacgcgt cggtggcgct gacgcgcgaa
     4081 ggccgctggc acacgcgagg catgcgcggc tccgccgtga gcgcggcgcg gcggcgcacg
     4141 aagtggcccg acttcggccg cacctcgatg agccctgcat tctcgagctg cagatacgct
     4201 tcgagcaccg tcgacacgct gacgctgcgc tcctgcgcga ggcgtcgcac cgacggcaac
     4261 cgatcgcccg ctcgtagtgc gcctcgatcg atcgcctggt tcagctcgtc cgcgagatgc
     4321 tcgtacagga acccggtggc caccatcgtg acgaaagtgt atccatcgac gtcgtgaccg
     4381 accagcacag atgcggggcg ctacaccggt acagcgcaac tgtactggtc cgcgcagcaa
     4441 attcgtgtct ctgtactgat cgcaggcagc cgtcatcatg aggcccgtgg tcacaacaac
     4501 gtcatcgcct gaggctgtcg cgcctgtcgc accgaagatc gacccacgtc tgatcgcgag
     4561 cctcgccgcg gtgtacgtga tctggagctc gacgtacctc gcgatgcgga tcgtcgtcca
     4621 cgatctgccg ccactgctca gcgcggcgct gcgcttcttg ctcgcgggcg ggacgatgtt
     4681 tggctacgcg ctgtacaagg gcgcgcgctt cccgtccctg cgcgattggc tgcgcgtcgc
     4741 tccggtcggc atcttcctgt ttgtcggcgg caacggattc gtctcgatcg catcgcagtc
     4801 gatcccgagc ggcggcattg ccgtcgtgtg cgcgctgatg ccgctgtggg ccggcgtgct
     4861 cggcttcgcg ttcggcgtcc gcccgacgag gcgcgagtgg ttctcgctgt tcatcggctt
     4921 cgccggcgtc gtgatcttgc tcggtggccc gtcgctcgca ggcaagccca tccacatcgc
     4981 gctcgtgatc gcgtcgccca tgctgtgggc tgcgggctcg ctgctgtcgc gccgaacgaa
     5041 agatgtcggc ggcgtgcacg ctccgctgat cggccctgcc ctgcagatgc tcacgggcgg
     5101 tgcagcgctc gccgccgtcg cgctcgtgcg cggtgaacgc ttcccggtcg acgcttccgc
     5161 gacgagttgg gccgcgctcg tctacttgtt catcttcggc tcgctggtcg cattcaccgc
     5221 gtacgcgtgg ctccttcgca acacgcgccc tgcgatcgct accagctacg catatatcaa
     5281 cccgatcctc gcggtcctca ttggtgctgc gctctacggc gagccgctgg gcgtaacgac
     5341 gctcattgcc aacatcctca tcgtcggcgc ggtcatgctc gcgttaagtg gcactcgcca
     5401 cagggtttag ctacgcttcg tggatgcttc gggtggtgct ccgccttctg cttgcctcgg
     5461 ccgtggtgac cacgctgccg gcttgcccgg gctctacgca gaccaagacg tccaacaaga
     5521 agaccaagaa ggcgaagaag gccgacacca aggcgctcgt atccgaagcg cgcgacgatg
     5581 cgaagaacgg cgactacgac gcggctgacg cgaagtacgc cgaagcgtgg gagacgtcga
     5641 aggacttcga tgtgctcgaa gagcgcgtcg acttcctcat tcacgcgggc cgcgcgacca
     5701 aggcagccga ggctgcgaag gcgtttctcg acgcgaacat cagcgacaag aacgcgtaca
     5761 tcctctacgc ggaggcgctg ctcgccggca acaagggagc agaagcgctc gaggtcgcag
     5821 accagctgat ccagctcaac gccgacgagc ctgcgggcta cgagaagaag ggccgcgcat
     5881 tgatcctcct cgagaagaac gaggaaggca tcgacatgct gcgcaaggcc gtgcagatgg
     5941 acaacacgtc ggcgtcattc catcgctcgc tcggcatcgc gctcaacaag ctcggcaaga
     6001 tcgacgagtc cgcactcgag ttccgcgccg ccatcaaggc cgattccgac gacgccgaag
     6061 cgcacgtgct cctcggcatg gcgctgcgcg gccagcagga atacgacgag gcgaagtcct
     6121 atctcgacaa agcgatcgag ctcgacccgc gcaacggtcg cgcgtacttc gagatgggcc
     6181 tgctctacaa ccagcagaag aagcaggacc tcgccgaagc ggcactgtcg aaggccgtgc
     6241 agctctcgcc gaacgagtcg ctgttctggt acgcgtacgg cgagatctat cgcctgcagg
     6301 agcgcttcga agacgcgatc acggcgtaca agaaggcgac ggacatcgac ccgccgtaca
     6361 cgaaggcgat gttcaagctc ggctcgctgc tcatccagaa gaagagctac gacgaggccg
     6421 aggtcgtgct gacaaacgcc gtgcgtcgcg agccgaagaa cccgagcggc tacctcgtcc
     6481 tcggcgatct ctacgcagcg aagcgcaagg cgaagctcgg catcgagagc taccagaagt
     6541 acctcgagct cgcgcccaag agcgatccag agcgcgatcg cgcccaatca gccatcagag
     6601 agctcaagcg ccaaaggtag ttagcggcgc atccgctatc gttgacaggt catccgccgc
     6661 gggtgtagaa caatatccgt tatgacggat gtcgggatcg acccgcctgc agccatcctg
     6721 cgctgggctg cgcgccacat cccgcgcctc gcatttgcga cgggcttcgg tgccgagggt
     6781 tgcgtgatca tcgatctgat cgcgcgcgag cagctgccga tcgacatctt cacgctcgat
     6841 accggcgtgc tcttccccga gacgtacgcg ctgtggaaag cgctcgaggc gaagtacggc
     6901 atcacgattc gcggcgtcgc gccggtgcag gcgatcgaca agctctggga gaccgacagc
     6961 gatcgttgct gcgatctgcg caaggtgcag ccgctgcgcg cggcactcgc cggcttcggt
     7021 ggctggatca cggcgatccg tcgcgaccaa acgccggagc gtgcgaacgc cgccgtcgtc
     7081 gaagccgacc cgaagttcgg cttgatcaaa gtgaacccgc tcgttgcgtg gacgcacgac
     7141 gacgtgtggg cccacctcta cgcgaacgat gtcccgacga acgcgctgca ccagcaaggc
     7201 tatccgtcga tcggttgcca gccgtgcacg tcgccgatcg tgccgggcga gaacctgcgc
     7261 gcgggtcgct ggcgcggcgc acagaagacc gagtgcggcc tccacacgcc gctgaaggaa
     7321 gcagccaagt gagcgacgcg cggctctatc ccgtcttcct caggctcgag ggactgcccg
     7381 tcgtcgtcgt cggtggcggg cccgttgcgg cgagcaagct cgacggtctg ctcgctgcag
     7441 gtgctcgcgt gactgtcatc gcaccgcacg tgttgcccga gattcgtgcg aaggcgaccg
     7501 tcgtcgaaca acctttccgc gcggcgcatc tcgcaggtgc gcgctgggtg atcgcggcgg
     7561 cgaccccaga cgtgaatcgc gatgtcgcgg cggcggcagc ggcgcgcggg ctcttcgtca
     7621 acgcggtcga cgacacgcgc gtcgcgacgg cgtacctagg tggcgtcgtg cggcgcggtc
     7681 ccgtcgagat cgcgatctcg acaggcggtc tcgcgccggc gctcgcgggc ctcttgcgcg
     7741 aagcgctcga tgcgctgctg cccgaagatc tcgacaagtg gatcgaagtc gcgaacgaag
     7801 cgcgccgcga ttggaagcaa cgcaaggtcc cgatgcccga gcgcaggcct ctgctgctgc
     7861 gcgcgctgga tcgcatttac gcaggagcct catcatgagc gggtttgttt ctctcgtcgg
     7921 tgcaggacct ggcgatcgcg agctgctcac gctgcgtgcg gccgatcggc tgcgcaacgc
     7981 agacctcgtg ctctacgacg cgctcacgcc cgaggacatc ctcgagctcg cgccgcaagc
     8041 caagcgtttc tacgtcggca agcgcgccgg ccgtcactcg atcgatcaag acggcattca
     8101 tcgcctcatg gtgcgcgcgg cgaatcgcgg cgagcgcgtc gtgcgactca agtgcggcga
     8161 tccattcgtg ctcggacgcg gcggcgaaga agcgctcgcg ctcgaagagg caggcatcgc
     8221 gtacgaagtg gtgccgggtc tgtcgtcggc gatcgccgcg cccgcgcttg ccggcattcc
     8281 ggtcacgcac cgcggcctct cgcagggctt cgcggtgttc tccggtcaca cgacgcagca
     8341 cgccgattcg atcgcgccgc acacgctgac gctcgtcgtg ctgatgggcc tgcgcacgcg
     8401 cgccgagctg gcggagcggc tcatcgctcg cggctggtcg ccggcgacgc ccgcagcgat
     8461 cgtgctcggc gcatcgcacg aaggcagcac gcgctggctc ggcacgctcg agacgctcgc
     8521 gacggccgat atcgatggtg acctcgcggg catcatcgtc gtcggcgctg tcgtcgagct
     8581 cgcaactcag atcgcgaatg actacacgag ccgaacgaag ctcgcagcga ggatctcatg
     8641 agtgtcacgg aagcacccgc acccgttccc gtcggccgcc tcggttttgc acgccacgag
     8701 gacgtcgacc tgttcgtcca acgcctcgaa gcattcgaga acggtgagct gtccccggac
     8761 gagtggcgct cgttccgttt gctgaacggc gtctacggcc agcgccaaga cggcgtgatg
     8821 atggtgcgcg cgaagctgcc cgctggcatc gcaacgcccg cgcagctgct cgcgctcgcc
     8881 gacgtcgccg agaaattctc cgccggtgaa cgagggcacg tgacgacacg gcagaacgtg
     8941 cagtttcact tcgtcccgca ggacaagacc gaagaagcgc tgcgcatcct cgccgattca
     9001 ggaatcacga cgaaggaagc gtgcggccac tccgtgcgca actggacgtg ctgcccgctc
     9061 gcgggtgtcg ccaagaccga ggcgttcgat ccgacgccgt atgtcgaggc gctcgcgcgt
     9121 cacctgttgc gcggcacgta cagctcgacg ctgcctcgca agctgaagcc atcgctcggc
     9181 ggttgctgtg gcaccgactg ctcccaggcg ttcatcaacg acctcggctt cctcgcgacc
     9241 tcgcgcgacg gcgcccatgg cttcaagctc ctcgcgggtg gcgggctctc gacgctgcgc
     9301 cgtgaagcga tcgtcgtcga agaattcgtc ccggttgcag aagtgctcga agccgctgat
     9361 gcgatcgtcc gcgtgttcca tcgcatcggc aatcgcaaca acaaggcaaa ggctcgcctc
     9421 aagtgggcga tcgaaaagat cggcgtcgat gcgttcctcg ccgagtatcg cgccgagcgc
     9481 gagacgatcc ggagcgaagg cggcgtgccg ctcgtgctgc cggcgcaacc agtgccaccg
     9541 tcgcgtcgtc cgttgctcgc tcaggtctcc gagccggccg acggctactc cgagtgggca
     9601 gccgacagcg tgcgcgcgca gaagcaagcg ggcttctccg ccgtcgtcat ccgcctcatc
     9661 ctcggtgaca tcacggcgac gcagctccgt gccctcgcgc agatcgcgaa gacgtacggc
     9721 gagggcgaag tccgcctgac gaacgagcag aacatcgtgc tgcgctgggt tcccgacgcg
     9781 cgcctgcccg cgcttcatcg cgagctcgca gcgatcggtc tcgcgaacat cggcgcgaac
     9841 acgctcgccg acgtgacgag ctgccccggc gcgacgagct gcaagctcgc ggtcacgtcg
     9901 agcaagggcc tcgctgcagt gctcacgcaa ttcctcgacg agcgccaaga cctcgtccgc
     9961 aaagcgaagg gcctcgacat caaagtgtcg ggttgcccgc acggctgcgg ccagcactac
    10021 atcggtggca tcggcttcca aggcggcatg cgcaagctcg ccggtcgcgc cgcgccgctg
    10081 tacctcgtct acgtcggtgg tggcatcacg gcgaccggtg ccgatttcgg caagctcatc
    10141 ggaaaagtgc cggcacgccg tgtcaccacg ctgctcgagc ggctgctcgg cttctacatc
    10201 gcggccggcg gcacgtcaca caagttctgg aacgacgtgc cggtcgacca ggttcgcgag
    10261 ctgatcgcag acctcaccgt catcgacgac gcgaccgcaa ccgaggaaga cttcatcgat
    10321 cacggcgaag cggctgcgtt cgaagtcatc accgccgaag gcgagtgctc cgcgtagcgc
    10381 tgatctagcg cgacgtcttg acgttcgggc gcgtcgcgtt gatgacgatg ccgatgatgc
    10441 cggccgtcac gagcatcacg cccgccacca tgccgccgcc gaatttcggc tgatagcagc
    10501 cattcatcga cgtcgtcggg cacggctcgt ctttcgagag aatgtctccc gcgatcgcgc
    10561 cgccaccgcc tgcgatgagg acggagttcc cggcgtatga ccatttcttc gcgcccgggt
    10621 tgtagccgaa gcatcccgga atcagcgcga ggatcgcgag cagagccacc gctctcatgc
    10681 gcagaccagt atatcctgcg cgcgctgtga gtcagaagcc gcggtcgccc ctgttgccga
    10741 tctttttgat cgtactggtc gacgtcctgg gattcacgat cgtgatcccg ctcctggcgt
    10801 tctacgccga gaagttcggt gcctcgccac tcgtcgcgac gacgctggtg tcggtctacg
    10861 ccgtctgctc gctgatctcg acgccgatca tcggcaacct ctccgaccgc tacggccgcc
    10921 gccgcctcct cctcgtcagc caggctggca cctgcctcgg ctttctcatg ctcgcgtggt
    10981 cgcacacgct gtggatggtg ttcctcggcc gcatcctcga cggaatcacc gccggaaacc
    11041 tgtcactcgc gcaggcgtac atctccgatc acaccaagcc cgaaaatcgc gcgaaggcgt
    11101 tcggcgtcat cggcgtcgcg ttcggcatcg gcttcatgtt cggccccggc atcggcggcc
    11161 tcctcggcaa gtacggcctc cacgtgccgt ttctcgtcgc agcgagcctc tcgctgctgt
    11221 cgatcatcgc gaccgcgacg ctcctcaaga acgaggttcc cggcgccccg accgaagccg
    11281 acgaggcgac gccggcgacc gagggtcagc ctccagcggg acgacgtccc ggcgcgttcg
    11341 acatcgcgac gtacaccgag tacttccgtc gccccaagct cggcaatctg tacctgcagt
    11401 tcttcctgtt cacgttcg
//
LOCUS       DATLBZ010000441         6168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3270929, whole genome shotgun sequence.
ACCESSION   DATLBZ010000441 DATLBZ010000000
VERSION     DATLBZ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3270929"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VMZ53_22315"
     CDS             complement(<1..737)
                     /locus_tag="VMZ53_22315"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HUS31269.1"
                     /translation="MSAVAVSPMTSEGKPRVVIANRADGGTAYWVGKLYGFAALVLAV
                     AIAITCLLLYRYFSERTDEVGPGTNHDLAAYAHHVPSVSHIYAADGTLLGEFASEWRE
                     FVSYEDIPKQMVDAFLAVEDHDYFNHRGIYFKGIARALWTNITAGDFAQGGSTITQQV
                     AKQFLGSEKSLSRKAKEAILARRLEATYSKRAILSVYLNQIYLGSGAWGVSAAARRYF
                     QKDLKDLTLAESALIAGLAKAPTAYSPV"
     gene            799..1227
                     /locus_tag="VMZ53_22320"
     CDS             799..1227
                     /locus_tag="VMZ53_22320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31270.1"
                     /translation="MRRLCLILVGISALGCRDPNAKKTSQGAAEAPASPTAQKQTTPD
                     PAPSVPAEQPRVERDQAWRDAMTKTIETVAPDLTAVTCGNIECSATLTAPSDEELVAR
                     AEKLQSEDSLRQLEARGVVLSAPIKKDGKSAMTIRVLFDE"
     gene            complement(1231..1887)
                     /locus_tag="VMZ53_22325"
     CDS             complement(1231..1887)
                     /locus_tag="VMZ53_22325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS31271.1"
                     /translation="MALLARVTSKNPADWTDRELLKWVLRSDARGWAELVRRYRPLIY
                     RCITKVTLKYAPSLGSADLDEIYADVMMQLVREDMHKLRIYNPARGTKLGSWIGMISV
                     NAAYDYLRSAGRRPLLDKVDGNLDPHEECDRTPLDQLIEKERWTHLNELLADFTEKDR
                     MFVELYYQKGMEADEIAAEMQISLKTVYSKKHKIRAHLVRCLGQIAGDSPIADLALAS
                     "
     gene            complement(2128..6141)
                     /locus_tag="VMZ53_22330"
     CDS             complement(2128..6141)
                     /locus_tag="VMZ53_22330"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS31272.1"
                     /translation="MNGDGCESDCKLPNGGTCDEDSDCHSGVCDPTSDVCEPANVCGN
                     GHVEGSEACDDGNTVGGDTCEANCKLPIGAPCTDDNQCNSGTCDTPGSDTCEPANTCG
                     NGHVEGTEACDDGNTSGGDTCEADCKLPNGSGCTTDNQCHSGVCDPSSHVCEPANTCG
                     NGHLDAGETCDDGNIVGGDTCEADCKLPNGSGCTTDTQCHSGVCDPTSHQCEPADTCG
                     NHKVENGETCDDGNTVGGDGCEADCKLPNGSTCTTDNQCHSGVCDPTSHQCEPANTCG
                     NGKRETGEGCDDGSTIPGDGCSALCKIEDGHPCNQTLPGDTGDDSCKSDLCDTTDGLP
                     GTCGGLDTDGDGVFDYADIDDDNDGLLDTMEGSGARDTDGDGAKDSRDLDADNDGIAD
                     ATEAGHRFPDNDGNFIADCPNRIYGVNGYCDALETTVDSGLPDFDGTGPSDGDPTNTD
                     ADSGPDYVDLDSDNDGIADLYEAGSGCADGNADGACDGDDVDHDGVAAALDFTLVTGT
                     QFGTSGGHIPTNTDGADAQDYRDLDSDQDGVFDILETKNAKLDVDKNGIIDATADRDG
                     DGVRDVADDSDLDGQPDSEDPDAPKFAGLHDPRISSDDDGTPDYQDPDSDGDNVGDFP
                     DNCRVDYNPDQMDVDGDGTGDACDERDGRGWGVQGSACGCDSSNGSPTGFLMVLGALG
                     LVVGRRRRRRSGAAGAALTLAALVLVPTTSHAQVVESDFSTERFQLASDREGILDVES
                     GSVRKHLQIDLAMWLGYANDPLILNRTDNGTMHVGSLVSNQVSGELVGSVGILNRAQV
                     ALAVPLIFAQTDSIPSGGATPTAPAGSFALGDLRLIPKYRLLPQSDFGVDLSFYISLT
                     FPTRSGEGFSGDRGVTAAPAFALSRSFPNGWRGAANLGYRIRGKEMSLDLEVNDEVFA
                     GLGVGKTLGKGEVDGGFTMATAANDMFGAFNRNYAELKAGGSYNVSGPVNLFAAMGAG
                     IAQGYGTPDWRALAGVRVDRKHEEKIVEPIRDTDGDGLLDNVDKCPTEPEDKDAFEDE
                     DGCPDVDDDKDGILDVNDKCRLEPEDKDSFEDEDGCPEADNDKDGILDPTDKCPVDPE
                     DLDKFQDDDGCPDPDNDNDTVLDPQDQCPEIAGPVENNGCPWPDSDGDGVIDKFDNCP
                     KWKGTPENQGCNIKQLVKITESKLEVYEATFFATNRSAIQKRSFKMLNNVAEVIKNHP
                     EIKISIEGHTDDRGSNKFNLKLSQSRADAVLKYFVKRGVEPAQLTAIGYGEEQPIADN
                     KTSKGRAANRRVEFMAQRVTTTTTTTVIQQDGTPAPTTTPPGTAPATPAPGATPAPAP
                     TAPTTPAPAPTAPAPKK"
BASE COUNT          970 a         1953 c         2024 g         1221 t
ORIGIN      
        1 accggcgagt acgcggtcgg cgcctttgcg agaccggcga tgagtgcgga ctccgcgagc
       61 gtcaggtcct tcaagtcttt ctggaagtag cgccgcgccg cagccgacac gccccacgcg
      121 cccgagccta gatagatctg gttcaggtac accgagagga tcgcgcgctt gctgtacgtg
      181 gcttcgaggc gccgcgcgag gatcgcttcc tttgctttgc gcgacaggct cttctcggaa
      241 ccgagaaact gtttggcgac ctgctgcgtg atcgtcgatc cgccctgcgc gaagtcaccg
      301 gcggtgatgt tcgtccagag cgcgcgcgcg atgcctttga agtagatgcc gcgatgattg
      361 aagtagtcgt gatcctcgac cgcgaggaac gcatcgacca tctgcttcgg gatgtcctcg
      421 tacgatacga actcgcgcca ctcggacgcg aactcgccga gcagcgtgcc gtcagcggcg
      481 tagatgtgcg acacgctcgg aacgtggtgg gcgtacgccg ccagatcgtg gttcgtgccg
      541 gggccgactt cgtccgtgcg ctcggagaag tagcgataca agagcaggca cgtgatcgcg
      601 atcgcgactg cgagcacgag tgccgcgaag ccgtagagct taccgaccca gtatgccgtt
      661 ccaccgtcgg cgcgattcgc gatgacgaca cgcggtttgc cctcggaagt catcggcgat
      721 actgcaacgg ctgacacggc cgagggattc tagccatcac gcgtccgaaa gcagcacttg
      781 ctgctaggct acgtcgcgat gcgccggctt tgcctgatcc tcgtcggaat ctcagcactt
      841 ggctgccgtg atcccaacgc gaagaagacc tcacaaggcg ccgccgaagc gcctgcatcg
      901 cccaccgcgc aaaagcagac gactccggat ccagcgccgt ccgtaccagc cgagcagccg
      961 cgcgtcgaac gagaccaagc gtggcgcgac gcgatgacga aaacgatcga gacggtcgcg
     1021 cccgatctca ccgcggtgac ctgcggcaac atcgagtgct ccgcgacgct gactgcgccg
     1081 tctgatgaag agctcgtcgc gcgcgctgaa aagctgcaga gcgaagactc gcttcgtcag
     1141 ctcgaagcgc gcggcgtggt gctcagcgct ccaataaaaa aggacgggaa gtcagcgatg
     1201 actatccgcg tcctgttcga tgagtaacgg ctacgaggcg agagcgagat ccgcgatcgg
     1261 gctgtcaccg gcgatctggc cgaggcagcg gacgaggtgc gcgcgaatct tgtgcttctt
     1321 cgagtagacc gttttgaggc tgatctgcat ctcggccgcg atctcgtcgg cttccatgcc
     1381 cttctgatag tagagctcga cgaacatgcg gtctttctcg gtgaagtccg cgaggagctc
     1441 gttgaggtgc gtccaacgct ctttctcgat cagctgatcg agcggcgtgc ggtcgcactc
     1501 ttcatgcggg tcgaggttgc cgtcgacttt gtcgagcagc gggcggcggc cggcgctgcg
     1561 caggtagtcg taggcggcgt tgacggagat catgccgatc cacgaaccga gcttcgtgcc
     1621 gcgcgccggg ttgtagatgc ggagcttgtg catgtcttca cgcacgagct gcatcatcac
     1681 gtcagcgtag atctcatcga ggtccgcgct gccgagcgac ggggcgtact tcagcgtcac
     1741 cttggtgatg cagcggtaga tgagtggccg atagcggcgc accagctcag cccagccacg
     1801 ggcgtcacta cgtagaaccc acttgagaag ctcacggtcg gtccagtcag cgggattctt
     1861 cgaagttacg cgggcgagaa gagccattgc gacctcctag tggaccgtgc tctagagcaa
     1921 cggtggtgcc acgcctaact gtgcgtaact gctacgacgt ggcgacgatc cacaggtggt
     1981 cgccaccatt ctttggtgaa aacgaccagg tgcgggaagt aagcgctggg aaggtccgta
     2041 ttaatcactc ccgtttcagc gccgctcccg ccgaaacgac gaaagcgcgc cgggttaggg
     2101 cgcgctgtag gtgctgcttc gcgtcggtta tttcttcggc gcgggagcgg tcggcgcggg
     2161 agccggagtg gtcggagcgg tcggcgccgg agcaggcgtc gcgcccggag caggcgtcgc
     2221 cggagcagtg ccgggcggcg tggtagtcgg tgccggcgtg ccgtcctgct ggatcacggt
     2281 ggtcgtcgtg gtcgtcgtca ctcgctgcgc catgaactcg acgcggcggt tggcagcgcg
     2341 acccttcgac gtcttgttgt ctgcgatcgg ctgctcctcg ccgtaaccga tcgccgtcaa
     2401 ctgtgccggt tcgacgccgc gcttgacgaa gtacttgagc accgcatctg cgcgcgactg
     2461 cgacaacttc aagttgaact tgttcgagcc gcgatcatcc gtgtggccct cgatcgagat
     2521 cttgatctcc gggtggttct tgatcacctc ggcgacgttg ttgagcatct tgaacgagcg
     2581 cttctggatc gcagagcggt tcgtcgcgaa gaacgtcgcc tcgtacacct cgagcttgct
     2641 ctcggtgatc ttgacgagct gcttgatgtt gcagccctga ttctcgggcg tgcccttcca
     2701 cttcgggcag ttgtcgaact tgtcgatcac accgtcgccg tcgctgtcgg gccacgggca
     2761 gccgttgttc tcgaccggac cggcgatctc gggacactga tcctgcggat cgaggacggt
     2821 gtcgttgtcg ttgtccggat ccgggcagcc gtcgtcatcc tggaacttgt cgaggtcttc
     2881 cggatcgacg ggacacttgt cggtcggatc gaggatgccg tccttgtcgt tgtcggcttc
     2941 ggggcagccg tcttcatcct cgaagctgtc tttgtcttcg ggctcgaggc ggcacttgtc
     3001 gttgacgtcg aggatgccgt ccttgtcgtc gtcgacgtcg gggcagccgt cttcgtcctc
     3061 gaatgcatcc ttgtcctcgg gctcggtcgg acacttgtcg acgttgtcga ggagaccgtc
     3121 accatcggtg tcgcgaatcg gctcgacgat tttctcctcg tgcttgcgat cgacgcgaac
     3181 gcctgcgagc gcgcgccagt ccggcgtgcc gtagccttgc gcgatgccgg cgcccatcgc
     3241 agcgaacagg ttcaccgggc cgctcacgtt gtagctgccg cccgccttga gctcggcgta
     3301 gttgcgattg aacgcgccga acatgtcatt cgcggcagtc gccatcgtga agccaccatc
     3361 gacttcaccc ttgccgagcg tcttgccgac gccgagaccg gcgaagactt cgtcgttgac
     3421 ctcgaggtcg agcgacatct ctttgccgcg gatgcggtag ccgaggtttg cggcgccgcg
     3481 ccagccgttc gggaatgacc gcgacagcgc gaacgctggt gcagccgtca cgccgcgatc
     3541 accgctgaag ccttcgccgc tgcgggtcgg gaacgtgagc gagatgtaga acgagaggtc
     3601 gacaccgaag tcgctctgcg gcaacaggcg atacttcgga atcaggcgca gatcgccgag
     3661 cgcgaagctg cccgccggtg ccgtcggcgt cgcaccaccc gacggaatgc tatcggtctg
     3721 cgcgaagatc agcggaaccg cgagagcgac ttgcgcacgg ttcaggatgc cgacggaacc
     3781 aacgagctcg ccgctcacct ggttcgagac cagcgagccg acgtgcatcg ttccgttgtc
     3841 ggtgcggttg aggatcagcg gatcgttcgc gtagccaagc cacatcgcga gatcgatctg
     3901 caggtgcttg cgcacgctgc ccgactcgac gtcgaggatg ccttcgcgat ccgatgcgag
     3961 ctggaagcgc tccgtgctga agtcgctctc gaccacctgc gcatggctcg tcgtgggcac
     4021 gagcaccagc gcagccagcg tgagcgccgc gccggcggca ccgctcctgc gacgtcggcg
     4081 gcgaccgacc acgaggccga gcgcgccgag caccatcagg aatccagttg gcgaaccatt
     4141 cgacgaatcg cagccgcacg cggagccttg aacgccccaa ccgcgaccat cgcgctcgtc
     4201 acaagcgtca cccgtgccgt ccccatcgac atccatctga tcaggattgt agtcgacgcg
     4261 gcaattgtca gggaagtcgc cgacgttatc tccgtcggaa tccggatcct ggtagtcagg
     4321 tgtgccgtcg tcgtcgctcg aaatgcgcgg gtcgtggaga ccggcgaact tcggcgcatc
     4381 gggatcctcg ctgtcgggct gaccgtcgag gtcgctgtcg tcggcgacat cgcgcacacc
     4441 gtcgccatcg cgatcggccg ttgcgtcgat gatgccgttc ttgtcgacat cgagcttcgc
     4501 gttcttcgtc tcgaggatgt cgaacacgcc gtcctggtcg ctatcgagat cgcgatagtc
     4561 ctgcgcgtcc gcgccgtcgg tgttcgtcgg gatgtgcccg ccggacgttc cgaactgcgt
     4621 accggtgacg agcgtgaagt cgagcgcggc tgcgacgcca tcgtgatcga catcgtcgcc
     4681 gtcgcatgca ccgtcagcat ttccgtcggc gcagccagag cccgcttcgt agagatccgc
     4741 gatgccgtcg ttgtcgctgt ccagatcgac gtagtccggg ccgctgtcgg catcggtgtt
     4801 ggtcggatcg ccgtcggacg ggccggtgcc gtcgaagtcg ggtaggccgg agtcgacggt
     4861 cgtctcgagc gcgtcgcagt agccgttcac gccgtagatg cggttcgggc agtccgcgat
     4921 gaagttgccg tcgttgtcgg ggaagcggtg gccagcctcg gtcgcatcgg cgatgccgtc
     4981 attgtccgcg tcgaggtcgc gcgagtcctt cgcgccgtca ccgtcggtgt cgcgcgcgcc
     5041 gctgccttcc atcgtgtcga ggagaccgtc gttgtcgtcg tcgatatctg cgtagtcgaa
     5101 gacgccgtcg ccgtcggtat cgagaccacc gcacgtgccg ggcaggccgt cggtcgtgtc
     5161 gcagagatcc gacttgcacg agtcgtcgcc ggtgtcaccg ggcagcgtct ggttgcacgg
     5221 atgaccgtct tcgatcttgc agagcgcgct gcaaccgtcg cccgggatcg tgctgccgtc
     5281 gtcgcagcct tcgccggttt cgcgcttgcc gttgccgcac gtgttcgccg gctcgcactg
     5341 atgcgaggtc ggatcgcaca cgcccgagtg acattggttg tcggtggtgc acgtcgagcc
     5401 gttcggcagc ttgcaatcag cttcgcagcc atcgccaccg acggtgttgc cgtcgtcgca
     5461 ggtctcgccg ttttcgactt tgtggttacc gcacgtgtcc gcgggctcgc actgatgcga
     5521 ggtcggatcg cagacgcccg agtgacactg ggtgtcagtc gtgcagcccg agccattcgg
     5581 cagcttgcag tcggcttcgc acgtatcgcc gccgacgatg ttgccgtcgt cgcatgtttc
     5641 gcccgcgtcg aggtgaccgt tgccgcacgt gttcgccggc tcgcagacgt gcgagctcgg
     5701 atcgcacacg ccggagtggc actggttgtc ggtcgtgcag cccgagccgt tcggcagctt
     5761 gcagtcggct tcgcacgtgt cgccgccgct cgtgttgccg tcgtcgcacg cttccgtgcc
     5821 ctcgacgtgg ccattgccgc aggtgttcgc gggctcgcat gtgtcggagc ccggcgtgtc
     5881 acacgtgcca gagttgcatt ggttatcgtc ggtgcaaggc gcgccgatcg gcagcttgca
     5941 gttcgcttcg cacgtgtcgc cgccgacggt gttgccgtcg tcgcacgctt cgctgccttc
     6001 gacatgaccg ttgccgcaga cgttcgcagg ctcgcacacg tccgatgtcg gatcgcagac
     6061 gcccgagtga cagtccgaat cttcgtcgca cgtgccgccg ttcggcagct tgcagtccga
     6121 ttcgcagcca tcaccgttca tgtggttacc gtcgtcgcaa ccttcgcc
//
LOCUS       DATLBZ010000442        15257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4261092, whole genome shotgun sequence.
ACCESSION   DATLBZ010000442 DATLBZ010000000
VERSION     DATLBZ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15257
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4261092"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VMZ53_22335"
     CDS             <1..1391
                     /locus_tag="VMZ53_22335"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HUS31273.1"
                     /translation="FLFSQCQAIHARSIVPLQDTPRLRVRFTAELTFPRELTLVMAAA
                     DRGRREDGSAAIHRFEMPQPIPPYLFAFAIGDLASRDLSPRCRVWAEPAVVDAAAWEF
                     ADVEAMIGAAEALFGPYDWERFDILTMPPSFPYGGMENPRLTFLTPTVIAGDRSLVSV
                     VAHELAHSWTGNLVTNANAEHFWLNEGFTMYAERRIIEALDGKDMAALQAALGRRELD
                     ESLARFAKTPEMTKLRPQLAGVDPDECYSLVPYEKGYLFLTQIEAAVGREAFSKWLRS
                     YLAAFRFGAITTEDFERHIEAALPGALAKANARAWIDGEGLPSNVVTATSARLAAIES
                     IGAAVPAREQTATWTPIEWALYLESLPRPASHETCRALDDQYKLTASSNFDVLIPWLT
                     LALKSGYSAVLPRVQQVLGEVGRMKYLRPLYTALAADPSTRPTAVAVFERNAATYHPI
                     ARQMVESVLASV"
     gene            1399..2823
                     /locus_tag="VMZ53_22340"
     CDS             1399..2823
                     /locus_tag="VMZ53_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HUS31274.1"
                     /translation="MRRWATLFIGLAACGRLGFGPTEETTDGSTASGDDANVDSVVPL
                     AINSGTTSMQASYGGTANEEGYDVAVSSTGAVAVTGTLQSSFMVGSTAVGSVAGDDII
                     VSAFAADGTPAWTSTLGGGGGDRGQAIAFDSAGNIYVSGLYASTVNFFGTNKTAAGMW
                     DMFVVSYAPSGAFRWVTTAGGVNGDFAYDVIADSAGNVIVTGYYDGAVDFGMGPLPAA
                     GANEAFVAKYNGSTGALLWARGFTSAGSDTGQGVAVDAAGRIYVTGNLGGAIDFGGGP
                     VGAAGGAYLLALDSAGNYLWATTMKNTLAWDVALDAGTSQLLVAARVSTAGTDVGDGM
                     LLPTYGGSDDNMVVAFTLNGTLAWHRESGGDLSDIGLGVTSDGTGNVYLIGLGGTALN
                     GAGANAIQGNGMQDIYVIAYDATHTPRWGKMYGGAAQDRGYAVAVAPDGALWITGRYA
                     GTADFGKGAVTSAGGNDLFLTKLY"
     gene            complement(2817..3626)
                     /locus_tag="VMZ53_22345"
     CDS             complement(2817..3626)
                     /locus_tag="VMZ53_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31275.1"
                     /translation="MKKLAVLVLAFTACAAEEEADYSELDDLDAIAGKADGFSSWQLV
                     GTGVAYQQVNTGHGVLIAYGGYTAKLSDSAAWASELVDAKLGAADVGHIFAVRGPADP
                     AYAAREIANTKLRAKLATFDDGTAPIYVVAHSSGSFVAHELLAQVNNASPPLLARTAY
                     ANLDGGGSGLTSTIANALAGLEFVYARDPGAGASHNAGPMMSLAAAYGAPAFEVRVPG
                     TGCSAGASWCLHDVVITHRPHNPSTFDLARDYTDFVNRTVTTEYLDPLLPQ"
     gene            complement(3711..5147)
                     /locus_tag="VMZ53_22350"
     CDS             complement(3711..5147)
                     /locus_tag="VMZ53_22350"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HUS31276.1"
                     /translation="MVAAAIPQPRRIAPQVDPSIEDVIGSASKPSGVPAQVTPPESLP
                     GKLGFWAGIQAVMGTMRHGVDWVVGQHTKYGELSRGTFLDRDMVFIWDADEIHKALRN
                     EGGAWSTGMGWDLLAFQGLEERKGNMGSLLALDFDDHRAARALVQPAFTMRAVQGYLD
                     TAKRSFDETVPAWVERGRVPFKRDVRTLLARVANQIFTGIEDPAQVAAIDRSLVDFWT
                     GMLALSRKPWLSPTFRRARRGYASLQDTFVRLAAERRDATTGNDLFSHMVRGAAPESF
                     DAIVRVFLTIMFGAFDTTSVACTSMAYLLAKHPEWQERVRTEALAFPSGALDAMSLRS
                     MKELEWVWKETLRLMPVSGFVPRRTLRAVEVGGVTLPPGTFAGIMQGAIGRHPKWWSE
                     PDRFDPERFAPDRAEDKQHPALQMPFGAGAHACIGNQLATMEMKLLFHKLLTTCRFSL
                     AKDYDARHTFTPMGCVSGKVDLRLEPLA"
     gene            5237..7864
                     /locus_tag="VMZ53_22355"
     CDS             5237..7864
                     /locus_tag="VMZ53_22355"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31277.1"
                     /translation="MGEPRRTRLPLQIGIQSLYSLAFASATLVPAAVALLATLFILVI
                     KGGGIILLLLFSQGWVIAVGIIGIEVMLLVRGILHLRFALRTRPADAVFDDRGLHVEG
                     GRLHGRSIAWSDCKAVREEKVGPADDEIGGSRAVIDRKQGEPLIVAEAEDESELASLR
                     ALCDSVRARLHPEALPLLPPEDRPDLLRCPSCAAAAAPIDADHTTCAYCSAQVPIPKA
                     LREKLRATEEVLASSKVSAPLLAKLLKQPSARYATLLSIVAGIPIAAMWILAVVFAVA
                     LYRHHGLRAINVVLLSVSALVTIAGCYYLLRFWFVNRMALSVITLSFGAFPPAKEGAP
                     WTCRSCNAPLPPARSGLLARCAFCGSDNVQGIDLRPRAEGARKERATLESAFARRDKD
                     RRNYALRAAAALVVLPISLWGFFRASKPVTREAKLLVACDRGELPVCVDLGRYYLETQ
                     TYNRDAAKRVFERACKRTSADACAELGALLASGDAKELARAVELTWSGCQANHAMSCT
                     VLGNMIEGRRVSRTDLPDAVALYRRACDAGHAHGCATLAEHVFAGNGVPKDRAAGVKL
                     FEQSCAAKDPFACADLAQLYFDGNGVPQDRARAIAMWSDACSTTPPTYAACSGLGFAY
                     EKGLGVVKDRTRSWDLLRRSCEYGNVAAACTRVGEIQEAQERYDAAFEHYVRACPSDA
                     GGCLGMASVEVKRGNASRGIALLRRSCTDDHVGEACTLGAIWLVNGKHGIAKDPAQAA
                     ELYAAGCNLDNASSCNLGGQLADTQQDYAAARRLLTRACDLGEASGCNFLGKELEDGL
                     GGAADASAALAAYQRACDTPLVDDSHGAQAYGCAHEGLLARKLGDEPRAVSRLRKACD
                     WESQPACDALQAAPDARAR"
     gene            complement(7834..8052)
                     /locus_tag="VMZ53_22360"
     CDS             complement(7834..8052)
                     /locus_tag="VMZ53_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31278.1"
                     /translation="MVLRPRVPFNGVKVFSATMFNDRQVLGEKVTQWIAENPLNELTE
                     IMVTQSSDASFHCIALTVFYRARASGAA"
     gene            complement(8184..9197)
                     /locus_tag="VMZ53_22365"
     CDS             complement(8184..9197)
                     /locus_tag="VMZ53_22365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUS31279.1"
                     /translation="MATLTERWLRPRTPRTQFPPYELGDPTIVGLAEARTSTIARIYH
                     KGHEQAWDGRAVLDELVAKHGGIHFPEDKKAAFGRIASVLLWGELAAWSISADLALKL
                     EETPAKMAASSQVFDEARHFYVLRDYMWRAGIPIDKLGGWSRRLLVELLETDNLMHKV
                     VGMQLLVESTAVVMFRMIAESKLEPVLSDLLYYFERDEARHVGLGVLTLPEVLAGLTE
                     RDAMRLWWFQTRMNLEMTLGGITLRHAFDKLGIDQAEMNMQGFKYHNEIIRRMRKESP
                     GTGADAASVKGLFRISRKGQDAFQEFFFPTKPRSPVSSRMFDAIVSVAETTDRWLRER
                     APA"
     gene            complement(9228..9851)
                     /locus_tag="VMZ53_22370"
     CDS             complement(9228..9851)
                     /locus_tag="VMZ53_22370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS31280.1"
                     /translation="MRSEARQRQILACAKRVFAERGFHAANISHICEAAGIGRGTLYQ
                     YFTNKRSVLTAILRETLDRVRALMEQQARETEYPPPEKVTRSAAVAVSTQQLRRVLAV
                     VFDDSDTLRILLREAVGLDVEVEKILSEIDEALIGIVERDVKRALAAGYLRELDARAI
                     ATMVVGGIEKLGLAALRSDAPVDLDKLAREAAMLHAIGTLSDRLKPE"
     gene            10140..10454
                     /locus_tag="VMZ53_22375"
     CDS             10140..10454
                     /locus_tag="VMZ53_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31281.1"
                     /translation="MTNTDDFETIPSELLTTAQGGATTWRQLARFANAHGYHVTSSTG
                     GRHLGWAHHAGRAIDVRTRGHSSADVNSFMRAARRAGITVFDERRGGNSAWSGPHLHL
                     QK"
     gene            complement(10457..11767)
                     /locus_tag="VMZ53_22380"
     CDS             complement(10457..11767)
                     /locus_tag="VMZ53_22380"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS31282.1"
                     /translation="MRALPAVALAAAIACGCGDTTTAPQLAPKPTPGEAAQWSDAWLL
                     AQTSSYVSDAAARRAALESSLANPANMYSRIRLASYGLESRGWDLLPVWNPRSIPVTA
                     ELLPALERGESPTLPAGQPPLWNGVTPSTMPEWIALGRRVFFEYPMRAEVFMEYALTD
                     SELAAAVGIQRTRDGALPGLVVFANVDGETRVGITCAICHSTVKNGEVVVGQARRAFD
                     YGRLRLTFDRVHGTVRSAEEAKRMASWGPGRADVTEDDDEDPVAIPDLWGLRHQRWLT
                     QAGTIRHDSPLALAIRQETQLTDSNHARVRPPRELAWALAMFVYSLSPPTTDAAPPAQ
                     GEALFREHCARCHANAAYGGNTIAAATIGTDPALATGRGRGTGKYRVPTLLDVRDGAP
                     YLHDGSVGSLAELLSPTRAAPGHRAGTQLVQDDRDALIAFLETL"
     gene            complement(11767..12339)
                     /locus_tag="VMZ53_22385"
     CDS             complement(11767..12339)
                     /locus_tag="VMZ53_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31283.1"
                     /translation="MMKHLIVILALSAVSCDKGTPPRAKATVTLTSVSFADDCGGTPP
                     SDAPPSPARREAPAAAAKERAEPLDEGARRRCEQTSMQLVVVGDAGTELHIKSVEVFD
                     ADGKSLGKLSASKPTRWNETNAAYEPWDEKVGAASSAVSYVLSQPSFVDQWDARDRTY
                     TVKVIASVAGVDQPLKTTVLVVGRPPPVPT"
     gene            complement(12339..12944)
                     /locus_tag="VMZ53_22390"
     CDS             complement(12339..12944)
                     /locus_tag="VMZ53_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31284.1"
                     /translation="MRRLVLLFVLVAGCEGGPRKEAAPIPPPKLATRVELTAVTLADD
                     CGGAPPASPPVKTERAETATKAKSKADSATFEGARMRRCEQTSMQLTIIAAEGSKIQV
                     SSVELFDDKGTSLGTLTASRPTRWSDASGTYEAWDESVTAGSTTSVSYVLTQPKWDHI
                     GDRWNKTYTVKTVLSVGGVTQAAERNVTLTAPTSLPPNVKT"
     gene            13138..13518
                     /locus_tag="VMZ53_22395"
     CDS             13138..13518
                     /locus_tag="VMZ53_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31285.1"
                     /translation="MGKSEPGDNPASAFERAARRLLAGRPSDSNSAKQVSARAAQSLE
                     RLSTHLARILGATGVHMLMERSIASSQFPHAMENQNPETITDAFVDVLSSFVGLLKKL
                     IGDGLVERLLHEVWPAIFVPAEHP"
     gene            13515..14519
                     /locus_tag="VMZ53_22400"
     CDS             13515..14519
                     /locus_tag="VMZ53_22400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS31286.1"
                     /translation="MKKPPESNDAEGAASAREKSVANRETWVRARERLTGEHEILLHD
                     REEAVTVREASMRARTEAEAARVEREHLLVQMREANEKLVMATLRAGESESAARASDR
                     AKDDFIARLGHELRNPLAPILIALDLIAMDGPDSHEREHAIIGRQVNHLIQLVDDLFD
                     VSRIRSGKLSLQCEPIEMADVIWGAVEMARPMIDAHAHVLTIEVPRQGLAVNGDAVRL
                     AQVVGNLLTNAAKFTPSGGTIAVMGERHDTTVVLRIRDTGIGISPQMLPRLFDAYVQQ
                     HRDASHVRSGLGLGLAIVRSLVEMHGGTVTAHSKGLGLGSEFLVGLPLVTIERSSDAA
                     "
     gene            complement(14539..>15257)
                     /locus_tag="VMZ53_22405"
     CDS             complement(14539..>15257)
                     /locus_tag="VMZ53_22405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS31287.1"
                     /translation="AGTWKNLPGYEVAQPAASNLTKVLRPDVPYNQLDGMWGVTGGSE
                     PAYNLSSYYGSTYKNNRQVTLLTGPNGPGDQDLWMSGYLDGCGDIILNQRPGVKHAQG
                     CEGKISYLAGHSYSTNVPVTSGSEGNGTRMFLNALFEAQCTTAAGGNDSDGDGVDDAN
                     DPDPTDPHKCGDGDADMCDDCASGHYDPINDCNAAGDAGLNPGTGDKSGCCQTGAGGG
                     ARSMLLAIVVGLLLVRRRRR"
BASE COUNT         2555 a         5033 c         5052 g         2617 t
ORIGIN      
        1 cgtttctgtt ctcgcagtgt caggcgattc acgcgcgctc gatcgtgccg ctgcaagaca
       61 cgccgcgcct gcgcgttcgc ttcaccgcgg agctgacgtt cccgcgcgag ctcacgctcg
      121 tgatggctgc ggcggatcgc gggcggcgcg aggatggcag cgcagcgatc catcgcttcg
      181 agatgccgca gccgattccg ccgtatctgt tcgcgttcgc gatcggtgac ctcgcgtctc
      241 gcgatctatc accgcgctgt cgcgtatggg cggagcccgc ggtggtcgac gcagcggcgt
      301 gggagttcgc cgacgtcgaa gcgatgatcg gcgcagccga ggcgctgttc ggcccctacg
      361 attgggagcg cttcgacatc ctgacgatgc ctccatcgtt tccgtatggc ggcatggaga
      421 acccacgcct gacgttcctc acaccgacgg tgatcgcggg cgaccgctct ctcgtctctg
      481 tcgtcgcgca cgagctcgct cactcgtgga ccggaaatct cgtgacgaac gcaaacgccg
      541 agcacttctg gctcaacgaa ggcttcacga tgtatgcgga gcggcgaatc atcgaagcgc
      601 tcgatggcaa ggacatggcg gcgctgcaag ccgcgctcgg tcgccgcgag ctcgacgaat
      661 cgctcgcgag attcgcgaag acgcccgaga tgacgaagct gcggccgcag ctcgccggcg
      721 tcgatccgga cgagtgctac tcgctggttc cgtacgagaa gggctacctg ttcctcacgc
      781 agatcgaagc cgcggtcggt cgcgaggcat tctcgaagtg gctgcgcagc tacctcgcgg
      841 catttcggtt cggcgcgatc acgacggaag atttcgaacg ccacatcgag gcggcgctgc
      901 ccggtgctct cgcgaaggcg aatgcccgcg cgtggatcga tggcgaaggc ctgccgtcga
      961 acgtggtcac cgcgacgtct gcgcgcctcg ctgcgatcga gtccatcgga gcagccgtgc
     1021 cggcgcgcga gcaaaccgcg acgtggacgc cgatcgaatg ggcgctgtat ctcgagagcc
     1081 tgccgaggcc cgcgtcgcac gagacgtgtc gcgcgctcga tgatcaatac aagctcaccg
     1141 ctagcagcaa cttcgacgtg ctcatcccgt ggctcacgct cgcgctgaag agtggctaca
     1201 gcgccgtgtt gccgcgcgtg cagcaggtgc tcggcgaggt cgggcgcatg aagtacctgc
     1261 gcccgctgta caccgcgctt gcggccgacc cgtcgacacg gcccaccgcg gtcgctgtct
     1321 tcgagcgcaa tgccgcgact tatcacccga tcgcacggca aatggtggag agcgtcctgg
     1381 ccagcgtatg atgtacgagt gcgtcgctgg gccacgctgt tcataggtct tgctgcatgc
     1441 ggccggttgg ggttcggccc aaccgaggaa accaccgacg gctcgactgc gagtggcgac
     1501 gatgcaaacg tcgatagcgt cgtgccgctc gccatcaaca gcggcaccac atcgatgcag
     1561 gcgagctacg gcggcactgc gaacgaagag ggctacgacg tcgcggtgag ctcgacgggt
     1621 gctgtcgcgg tgaccggcac gctgcagagc tcgttcatgg tcggttcgac cgcggttggc
     1681 agcgtcgcgg gcgacgacat catcgtctcg gcgtttgcgg ccgatggaac cccggcgtgg
     1741 acgagcaccc tcggtggtgg cggtggagat cgcgggcagg cgatcgcgtt cgacagtgca
     1801 ggcaacatct acgtcagcgg gctctacgcg agcaccgtga acttctttgg cacgaacaag
     1861 acggctgctg ggatgtggga catgttcgtc gtgtcttatg cgccgagcgg cgcgttccgc
     1921 tgggtcacga ccgcgggcgg cgtcaacggc gacttcgcgt acgacgtcat cgccgactcc
     1981 gccggcaacg tcatcgtcac cggctattac gacggcgcgg tcgacttcgg catgggcccg
     2041 ctgccggcgg ccggcgccaa cgaagcgttc gtcgcgaagt acaacgggtc gaccggcgcg
     2101 ctcttgtggg cacgcggctt cacgagcgca ggctcggaca ccgggcaagg cgtggccgtc
     2161 gacgcggctg gacgcatcta tgttacgggc aacctcggcg gtgcgatcga cttcggtggt
     2221 ggtccggtcg gagctgcagg cggtgcgtat ctgctcgcgc tcgattcggc ggggaactac
     2281 ctctgggcaa cgacgatgaa gaacaccctc gcgtgggatg tcgcgctcga tgccggcacg
     2341 tcgcagcttc tcgtcgcggc gcgcgtctcc acggctggca ctgatgttgg tgacggcatg
     2401 ttgctgccaa cgtacggcgg cagtgacgac aacatggttg tcgcattcac gctgaacggc
     2461 acactcgcat ggcaccgcga gagcggcggt gacttgtcgg acatcgggct cggcgtgacg
     2521 agtgatggca ccggcaacgt ctatctgatc ggccttggcg gcactgcgct caatggcgcc
     2581 ggcgcgaacg cgatccaggg caacgggatg caggacatct acgtcatcgc ctacgacgcc
     2641 acgcacacgc cgcgttgggg aaagatgtac ggcggggcag cgcaggatcg cggctatgcg
     2701 gtggcggttg cgcccgacgg tgcgctctgg atcacggggc gctacgcggg caccgccgac
     2761 tttggcaaag gtgcagtgac gagcgccggc ggcaacgact tgttcctgac gaagctctac
     2821 tgaggcagca gcggatcgag atactccgtc gtgacggtgc gattgacgaa gtccgtgtag
     2881 tcgcgcgcga gatcgaacgt cgatgggttg tgcggccggt gcgtgatcac gacgtcgtgc
     2941 aagcaccagc tcgcgccggc gctgcagcca gttcccggca cgcgcacttc aaacgcgggc
     3001 gcaccgtacg cagccgcgag gctcatcatt ggacctgcgt tatgcgaggc gccggcgcct
     3061 gggtcacgcg cgtagacgaa ttcgagaccc gcgagcgcgt tggcgatcgt cgaggtcagc
     3121 cccgagccgc cgccgtcgag gtttgcgtac gccgtgcgcg cgagcagcgg aggactcgcg
     3181 ttgttgacct gcgcgagcag ctcgtgcgcg acgaacgatc cgctcgaatg cgcgacgacg
     3241 tagatcggcg ccgtgccgtc gtcgaatgtc gcaagcttcg cgcgcagctt cgtgttcgcg
     3301 atttctctcg ccgcgtaggc agggtccgcc ggtccgcgca cggcgaagat gtggccgaca
     3361 tccgccgcgc cgagcttcgc gtcgacgagc tcgctagccc acgctgcaga atccgacagc
     3421 ttcgcggtgt agccgccgta cgcgatgagc acgccgtggc cggtgttgac ctgctggtac
     3481 gcgacgccgg tgccgacgag ctgccaggag gagaagccgt ccgctttgcc ggcgatcgcg
     3541 tcgaggtcgt cgagctcgct gtagtcagct tcttcttccg cagcgcaggc cgtgaacgcg
     3601 agcacgagca cggcgagctt cttcacgcgc gcatcgtcgc tgccgtcgag gagccacgct
     3661 ctgcgcaacc gctaacaacg cgacgtgcag cggggtgcgc aactgacaag ctacgcgagc
     3721 ggctcgaggc gcaggtcgac cttgcccgag acgcagccca tcggcgtgaa cgtgtgacgc
     3781 gcgtcgtagt ccttcgcgag cgagaaccgg cacgtggtga ggagcttgtg aaagagcagc
     3841 ttcatctcca tcgtcgcgag ctggttgccg atacacgcgt gcgcgccggc gccgaacggc
     3901 atctgcaggg cagggtgctg cttgtcctcg gcgcggtccg gggcaaatcg ctcgggatcg
     3961 aaacgatcgg gctcgctcca ccacttcgga tgcctgccga tcgcgccctg catgatgccg
     4021 gcgaacgtgc ccggtggaag cgtgacgccg ccgacttcaa ctgcccgcag cgtgcgtcgc
     4081 ggcacgaacc ccgacaccgg catcagccgc agcgtctcct tccacaccca ctcgagctct
     4141 ttcatcgagc gcagtgacat tgcgtccagc gcgccgctcg ggaacgcgag cgcttcggtc
     4201 cgcacgcgct cctgccactc gggatgcttc gcgagcaagt aggccatgct cgtgcaggcg
     4261 accgacgtcg tgtcgaacgc gccgaacatg atcgtgagga acacgcggac gatggcatcg
     4321 aagctctcgg gcgcagcgcc gcgcaccatg tggctgaata ggtcgttgcc ggtcgtggcg
     4381 tcgcgccgct cggcggcaag gcgcacgaac gtgtcttgga gcgacgcgta gccgcgacgc
     4441 gcgcggcgaa acgtcggcga gagccacggc ttgcgcgaga gcgcgagcat gccggtccag
     4501 aaatcgacga gcgagcggtc gatcgcggcg acctgcgcag gatcttcgat gccggtgaag
     4561 atctggtttg cgacgcgcgc gaggagcgtg cggacgtcgc gcttgaacgg aacgcggccg
     4621 cgctcgaccc atgcaggcac cgtctcgtcg aacgaacgct tcgcggtgtc gaggtagcct
     4681 tgcaccgcgc gcatcgtgaa ggcgggctgc acgagcgcgc gtgctgcgcg gtgatcgtcg
     4741 aagtcgagcg cgaggagcga tcccatgttg cccttgcgct cttcgagccc ttgaaacgcg
     4801 agcaggtccc agcccatgcc ggtcgaccac gcgccacctt cgttgcgaag cgccttgtgg
     4861 atctcgtcgg cgtcccagat gaagaccatg tcgcgatcga ggaacgtgcc gcgcgacagc
     4921 tcgccgtact tcgtgtgctg gccgacaacc cagtcgacgc cgtggcgcat cgtacccatg
     4981 acggcctgga tgcccgccca gaagccgagc ttgcccggca gcgattccgg cggcgtcact
     5041 tgtgcgggaa cgccgctcgg tttgctcgcg ctgccgatga cgtcttcgat cgacggatcg
     5101 acctgcggag cgatgcggcg gggctgggga attgcggctg cgaccatcgt gaagaggcag
     5161 agacggcccg gcccgaatgt gaaaagattt ttggccgcgc ggcgtaagtc cacggcggtg
     5221 cagtactctg cgcgcagtgg gcgaaccacg tcgtacccgc ctcccgctgc agatcggtat
     5281 ccagtcgctc tacagcctcg cgtttgcgag cgcgacgctg gtgccggcag ccgtagcgct
     5341 cctcgcgacg ctgttcattc tcgtcatcaa gggcggcggc atcatcctgc tcttgttgtt
     5401 ctcgcaaggc tgggtgatcg ccgtcggcat catcggcatc gaggtgatgc tgctcgtccg
     5461 tggcatcctg catttgcgat ttgcacttcg cacgcggccg gcggacgcgg tattcgacga
     5521 tcgagggctg cacgtcgagg gcggtcggct gcacggccgc tcgatcgcgt ggagtgactg
     5581 caaagccgtg cgcgaggaga aggtcgggcc cgccgatgac gagatcggtg gctcgcgtgc
     5641 ggtgatcgat cgcaagcaag gtgagccgct gatcgtcgcc gaggccgagg acgagagcga
     5701 gctcgcgtcg ctgcgcgcac tctgtgacag cgtgcgtgcg cggctgcatc ccgaagcgct
     5761 cccactactg ccgccggaag accgtcctga cctgctgcgg tgtccgtcat gcgctgccgc
     5821 agctgcgccg atcgatgccg accacacgac gtgcgcgtat tgctccgccc aggtgccgat
     5881 tccgaaggcc ttgcgcgaga agctgcgcgc gaccgaggag gtgctcgctt cgtcgaaggt
     5941 ctcggcgcca ctgctcgcga agctgctgaa gcaaccgagt gcgcgctatg cgacgttgct
     6001 gtcgatcgtc gcgggtatcc cgatcgcggc gatgtggatc ctcgcggtcg tgtttgcggt
     6061 cgcgctgtat cgccatcacg gtctgcgcgc gatcaacgtc gtactgctgt cggtatcggc
     6121 gctcgtgaca atcgcaggct gctactacct gctgcggttt tggttcgtca accgcatggc
     6181 gctgagcgtg atcacgctga gcttcggcgc gttcccgccg gcgaaggaag gtgcgccgtg
     6241 gacgtgtcgc agctgcaacg cgccgctgcc acccgcgcgg tcgggcctac tcgcgcgctg
     6301 cgcgttctgc ggctcggaca acgtgcaagg aatcgatctg cgcccgcgcg cggaaggagc
     6361 gcgcaaagag cgcgcgacgc tcgagagtgc gttcgctcga cgagacaagg accgtcgcaa
     6421 ctatgcactg cgcgcagcgg ccgctctcgt cgtgctgccg atctcgctgt ggggattctt
     6481 tcgcgccagc aagcccgtga cgcgagaggc gaagctgctg gtcgcctgcg accgcggtga
     6541 gctgccggtg tgcgtggatc tcggtcggta ctatctcgag acgcagacgt acaaccgcga
     6601 cgccgcgaag cgcgtgttcg agcgcgcctg caagcgcacc agcgccgatg catgtgcaga
     6661 gctcggtgca ctgctcgcgt ccggcgacgc caaggagctc gcgcgcgccg tcgagctgac
     6721 gtggagcggc tgtcaggcga accacgcgat gtcgtgcacc gtgctcggca acatgatcga
     6781 agggcgtcgt gtctcgcgga ccgatctgcc ggacgcggtc gcgctctatc gccgtgcgtg
     6841 cgatgcgggt cacgcgcacg gttgcgcgac gcttgccgag cacgtgttcg caggcaatgg
     6901 cgtgcccaaa gatcgcgcgg ccggcgtgaa gctgttcgag cagagctgcg ccgcgaagga
     6961 cccgttcgcg tgcgcggacc tcgcgcagct gtacttcgac ggcaacggcg tgccgcagga
     7021 tcgcgcgcgt gcgatcgcga tgtggagcga tgcctgctcg acgacgccgc cgacgtacgc
     7081 cgcgtgctcg ggcctcgggt tcgcgtacga gaagggcctc ggcgtcgtga aggatcgtac
     7141 ccgctcgtgg gacctgctgc gccggtcgtg cgagtacggc aacgtcgctg cggcatgcac
     7201 gcgcgtcggc gagatccaag aagcgcagga gcgatacgac gccgcgttcg agcattacgt
     7261 ccgcgcatgc ccgtccgacg caggcggctg cctcggcatg gcgtcggtcg aagtgaagcg
     7321 aggcaacgcc tcgcgcggca tcgcgctcct tcgccgctcg tgcaccgacg atcacgtcgg
     7381 cgaagcctgc acgctgggcg cgatctggct cgtcaacggc aaacacggca tcgcgaagga
     7441 tcctgcacag gcagccgagc tctacgctgc gggctgcaat ctcgacaacg cgagctcgtg
     7501 caacctcggc ggtcagctcg ccgacacgca gcaagactac gccgccgcgc gtcgcctcct
     7561 gacgcgtgcg tgcgacctcg gcgaggcgag cggctgcaac ttcctcggca aggagctcga
     7621 ggacggcctc ggcggcgctg ccgatgcatc cgccgcactc gccgcctatc aacgtgcgtg
     7681 tgacacgccg ctcgtcgacg acagccatgg cgcgcaggcg tacggctgcg cccacgaagg
     7741 tctgctcgct cgcaagctcg gcgacgaacc gcgagcggtc agccgactac gcaaggcgtg
     7801 cgactgggag agccagcccg cctgcgacgc gcttcaggcc gcgcccgacg cacgcgcacg
     7861 gtagaacacg gtcagcgcga tgcagtgaaa cgacgcgtcg ctcgactgcg tcaccatgat
     7921 ttcggttagc tcgttcagcg ggttctccgc gatccattgc gtgacctttt caccgagcac
     7981 ctggcggtcg ttgaacatgg tcgccgagaa cactttcacg ccattgaatg gcacgcgagg
     8041 ccggagaacc acgcggcctt ccgaattttg aagcatcagt tcgaatacca ggttgtgggc
     8101 gataagcgag gggctacaca aactgtcatg ccggctgcga gctattcgcc tgcgacgtga
     8161 gctgtgtcgc acctgttcgg gctctacgcg ggtgcgcgtt cgcgcagcca gcggtcggtc
     8221 gtctcggcga cgctgacgat cgcgtcgaac attctcgacg agaccggcga ccgtggcttc
     8281 gtcgggaaga agaattcctg aaacgcgtct tgtcctttgc gcgagatccg gaacagcccc
     8341 ttcaccgacg cagcatccgc ccccgtcccc gggctctcct tgcgcatgcg acgaatgatc
     8401 tcgttgtgat acttgaagcc ctgcatgttc atctcggcct gatcgatgcc gagcttgtcg
     8461 aacgcatggc gcagcgtgat gccgccgagt gtcatctcca agttcatgcg cgtctgaaac
     8521 caccacagcc gcatcgcgtc gcgctcggtc aacccggcca gcacctccgg caacgtcagg
     8581 acaccgagcc cgacatggcg cgcttcgtcg cgctcgaagt agtacagcag gtccgacaag
     8641 acgggctcga gcttggactc cgcgatcatg cgaaacatga cgaccgccgt cgactcgacg
     8701 agcagctgca tgccgacgac cttgtgcatc agattgtccg tctcgagcag ctcgacgagt
     8761 agccggcgcg accatccgcc gagcttgtcg atcggaatcc cagcccgcca catgtaatcg
     8821 cggagcacgt agaagtgccg cgcttcgtcg aagacctgac tcgacgcggc catcttcgcc
     8881 ggcgtctcct cgagcttcag cgccaagtcc gcgctgatcg accacgccgc tagctcgccc
     8941 cacagcagca cggacgcgat gcgcccgaac gccgccttct tgtcctcagg aaaatgaatg
     9001 ccgccatgct tggcaacgag ctcgtcgagc accgcgcgcc catcccacgc ttgctcgtgt
     9061 cccttgtgat agatgcgcgc gatcgtgctc gtgcgtgctt ccgccaaccc gacgatcgtc
     9121 ggatcgccga gctcgtacgg cgggaattgc gtgcggggcg tgcgtggccg tagccaacgt
     9181 tcagtcaaag tcgccatacg tcgttcgcct ccatcgtcgt tggtcattca ctccggcttg
     9241 agccggtccg aaagcgttcc aatcgcgtga agcatcgcgg cctcacgcgc gagcttgtcc
     9301 aagtcgaccg gcgcgtcgct gcgcaaggcc gccaagccga gcttctcgat gccgccgacc
     9361 accatcgtcg cgatcgctcg cgcatcgagc tcgcgcaaat acccagctgc aagcgcgcgc
     9421 tttacgtcgc gctcgacaat gccgatcaac gcctcgtcga tctcgctcaa gatcttctcg
     9481 acctcgacat cgaggccgac cgcctcgcgc agcagaatcc gcagcgtgtc gctgtcgtcg
     9541 aacacgaccg ccaacacccg gcgcagctgt tgcgtcgaca cggccaccgc cgccgaccgc
     9601 gtgactttct ccggcggcgg atactccgtc tcgcgcgcct gttgctccat caacgcccgc
     9661 actcgatcga gcgtctcgcg caagatcgcg gtcagcaccg agcgcttgtt cgtgaagtac
     9721 tgatagagcg ttccgcgccc gatcccggcc gcctcacaga tgtggctgat gttcgccgca
     9781 tgaaatccgc gctccgcgaa cacgcgcttc gcacaggcca gaatctggcg ctgccgcgct
     9841 tctgaacgca tataactcat ggttttcgca cagaactgac gtgtcagttc taaggataag
     9901 ggacggacgc aacttccgca acccacaatc gcgcgctggc tcctcgagtt gctggtgctg
     9961 gctgggagac cgcccccaat cacgcgtcgc ccgcaggcaa cacgcccatc gcgagcacga
    10021 gcaatcgcaa acttccgcgc gtgcagccgt cgcctctaga tcgcgatcga gtccagcgga
    10081 acgcacgtgc tcgcgggcac cgcagctgca cggagtcgca ggcgaaagga acaacagcca
    10141 tgacgaacac cgacgacttc gagacgattc ccagtgagct cctcacgacc gcgcagggcg
    10201 gtgcaacgac gtggcgccag ctagcgcgat ttgccaacgc tcacggctat cacgtgacgt
    10261 cgtcgaccgg cggtcgccat ctcggctggg cgcaccacgc aggtcgcgcg atcgatgtcc
    10321 gcacgcgcgg ccatagctct gccgacgtga attcgttcat gcgcgcagct cgccgcgcag
    10381 gcatcaccgt gttcgacgag cgtcgcggtg gcaactccgc atggagcggc ccgcacctcc
    10441 acctgcagaa gtagcgttac aacgtctcga gaaacgcgat cagcgcgtcg cggtcatcct
    10501 gcacgagctg ggtgccagca cgatgaccgg gcgcagcgcg cgtcggagac aacagctcgg
    10561 ccagcgagcc gacggagcca tcgtggagat acggtgctcc gtcgcgcacg tcgagaagcg
    10621 tcgggacgcg gtacttgccg gtgccacgac cacggccggt cgccagcgct ggatccgtgc
    10681 cgatcgtggc ggcggcgatc gtatttcccc catacgcggc attcgcatgg caacgcgcgc
    10741 aatgttcgcg gaacaacgct tcgccttgag ccggcggtgc cgcgtcggtc gttggtggtg
    10801 acagcgagta gacgaacatc gcgagcgccc acgcgagctc gcgtggcggg cgaacgcgtg
    10861 cgtggtttga atcggtcagc tgcgtctctt gtcggatcgc gagcgcgagt gggctgtcgt
    10921 ggcgaatcgt gccggcttgc gtgagccagc gttggtggcg cagcccccag agatccggaa
    10981 tcgccaccgg atcctcgtcg tcgtcctcgg tgacgtcggc acggccgggc ccccaactgg
    11041 ccattcgttt cgcttcctcg gcgctgcgca cggtgccgtg cacgcgatcg aacgtcagcc
    11101 gcagccttcc gtagtcgaac gcgcgacgtg cctgcccgac gacgacctcg ccgttcttca
    11161 cggtcgagtg acagatcgcg caggtgatgc cgacgcgcgt ttcgccatcg acattcgcaa
    11221 atacgacgag cccggggagc gcgccgtctc gcgtgcgctg gatgccaacc gcagcggcga
    11281 gctcgctatc cgtcagcgcg tactccatga atacctcggc gcgcatcggg tactcgaaga
    11341 acacgcggcg accaagcgcg atccattcag gcatcgtcga cggcgtgacg ccattccaga
    11401 gcggcggctg accggcgggt agcgtcggcg actcaccgcg ctctagagcc ggaagcagct
    11461 ccgcggtcac cgggatcgag cgcggattcc agacgggcaa cagatcccag ccgcgagatt
    11521 ccaagccata cgaggcgagc cgaatgcgcg aatacatgtt cgcgggattc gcgagcgatg
    11581 actcgagcgc cgcccgacgc gcggccgcat cgctgacata cgacgaggtc tgcgcgagca
    11641 gccaggcgtc ggaccactgc gcggcttcgc cgggcgttgg cttcggcgca agctgcggag
    11701 ccgtcgtcgt gtcgccacaa ccacacgcga tcgcggcggc gagcgcaact gcaggaagcg
    11761 cgcgcatcac gtcggaacgg gcggcgggcg accgacgacc aacaccgtcg tcttcagcgg
    11821 ctggtcgaca ccggcgaccg aggcgatgac cttcaccgtg tacgtgcgat cgcgtgcgtc
    11881 ccattggtcg acgaacgacg gctgcgagag cacatagctg accgccgacg aagcggcacc
    11941 gaccttctcg tcccacggct cgtacgccgc gttcgtctca ttccaccgcg tcggcttact
    12001 cgccgacagc ttgccgagcg atttgccgtc cgcgtcgaag acctcgaccg acttgatgtg
    12061 gagctccgtg ccggcgtctc cgacgacgac gagctgcatc gacgtctgct cgcagcgcct
    12121 gcgcgcacct tcatcgagtg gctccgccct ttctttcgcg gccgcggccg gcgcctctct
    12181 gcgcgcgggt gaaggcgggg catcagacgg cggtgtgccg ccacagtcgt ccgcgaacga
    12241 gaccgacgtg agcgtgaccg tcgccttcgc gcgaggtggg gtccccttgt cgcagctgac
    12301 ggctgacagc gccaggatca cgatcagatg tttcatcatc aggttttcac gttgggtggg
    12361 agactcgtcg gtgcggtgag cgtgacattg cgctctgccg cctgcgtgac accgccgacg
    12421 gacagcacgg tcttcaccgt gtacgtcttg ttccagcgat cgccgatgtg atcccacttc
    12481 ggttgcgtga gcacgtagct gacgcttgtc gtcgagcccg ctgtcaccga ctcgtcccac
    12541 gcttcgtacg tgccgctcgc gtccgaccat cgcgtcggcc tactcgcagt cagtgtaccg
    12601 agcgacgtgc ccttgtcgtc gaacagctcg acggagctga cctgaatctt cgacccttcc
    12661 gcggcgatga tcgtgagctg catcgacgtc tgctcgcaac gcctcatgcg tgcgccctcg
    12721 aacgtcgcgg aatctgcctt cgacttcgcc ttcgtcgcag tttcggctct ctcggtcttc
    12781 acgggcgggc tcgccggcgg cgcgccgcca cagtcatcgg cgagcgtgac tgcggtgagc
    12841 tcgacgcgcg tggccagctt cggcggtggt atcggtgccg cttctttccg ggggccgccc
    12901 tcgcagcccg caacaaggac gaacaggaga accaggcgtc tcatcgtcga tccaacgtaa
    12961 cacgcgccca ggtggtctaa ttttgccgaa cgcttcgagc gtccgcgcac gaaatgatcg
    13021 cgttgtgagg cgcaagcgtc tcgtcggcgc gtcgttcgcc acgaatcttc gattcgcgtg
    13081 gtgtcacgcg ttgacggccc gacgagagcg ggtcatgcat gatcgaaggc gggcgacgtg
    13141 ggaaaatcgg agccggggga caacccggcg agcgcgttcg agcgagctgc gaggcggctc
    13201 ctcgcagggc ggccgagcga cagcaattcg gcaaaacagg tgtcagcgcg tgcggcgcag
    13261 tcactggaac ggctctcgac gcacctagcg cgaatacttg gcgcgaccgg cgtccacatg
    13321 ctcatggagc ggagcatcgc gagctcgcag tttcctcatg cgatggagaa tcagaaccca
    13381 gaaacaatca cggacgcatt cgtcgacgtc ttgtcgtcat tcgtcggttt gctgaagaaa
    13441 ctcatcggcg acgggctcgt cgagcggctg cttcacgagg tctggcccgc catcttcgtg
    13501 cccgcggagc acccatgaag aagcctcccg agtcgaatga tgccgaaggt gcggcaagtg
    13561 cacgtgagaa gtctgtcgcc aatcgggaga cctgggttcg cgcccgagaa aggctcacgg
    13621 gcgagcacga gatcctgttg cacgaccgcg aggaggccgt aacggttcgc gaggcatcga
    13681 tgcgcgcgcg aacggaggcg gaggccgcac gcgtcgagcg tgaacacctg ctcgtgcaga
    13741 tgcgagaagc gaacgagaag ctcgtcatgg ccacgctgcg agcgggcgag agcgagagcg
    13801 ccgcgcgcgc cagcgatcgc gcaaaggacg acttcatcgc gcggctcggc cacgagctgc
    13861 gaaatccact cgcgccgatt ctgatcgctc tcgacctgat cgcgatggat ggccccgact
    13921 cacacgagcg cgagcacgcg atcatcgggc gacaggtcaa ccacctgatt cagctcgtcg
    13981 acgatctgtt cgatgtatcc cggatcagga gtggcaagct ctcgttgcag tgcgagccaa
    14041 tcgagatggc cgatgtcatc tggggcgccg tcgagatggc ccgtcccatg atcgacgcgc
    14101 acgcacacgt gctgacgatc gaagtgcccc gccagggtct tgccgtcaac ggcgatgctg
    14161 ttcgtctggc gcaggtagtc ggcaatctcc tgaccaacgc tgcgaagttc accccgagtg
    14221 gcggcacgat cgcggtcatg ggtgaacgtc acgacacgac cgtcgtgctt cgaattcgcg
    14281 ataccggcat cggaatctcg ccgcagatgc taccgcgctt gttcgatgcc tacgtccaac
    14341 agcatcgcga cgccagtcac gttcgaagcg ggctgggcct cggcctcgcg attgtccgct
    14401 cgctcgtcga aatgcacggc ggcacggtga ccgcacacag caaagggctc gggctcggca
    14461 gcgagttttt ggtcgggctc ccgttggtaa cgatcgaacg cagcagcgat gcggcgtgag
    14521 agcgttgcct ctgaccgcct aacgacggcg acgcctcacg agcaagagcc cgacgacgat
    14581 cgctaggagc atcgaccgtg cgccgccccc tgcaccggtt tggcagcaac cactcttgtc
    14641 gccggtgccc gggttgaggc cggcatcgcc tgccgcgttg cagtcgttga tcggatcgta
    14701 gtggccgctc gcgcagtcgt cgcacatgtc cgcgtcgcca tcgccgcact tgtgtggatc
    14761 ggtcggatcc ggatcgttcg cgtcgtcgac accgtcgccg tcggagtcgt tgccgccggc
    14821 cgcggtcgtg cactgcgcct cgaacagcgc gttgaggaac atgcgcgtgc cgttaccttc
    14881 ggagccggat gtcaccggca cattggtgct gtacgagtgg ccggctagat agctgatctt
    14941 accttcgcaa ccctgcgcgt gcttcacgcc ggggcgctgg ttgaggatga tgtctccgca
    15001 gccgtcgaga taaccgctca tccagaggtc ttgatcaccg gggccattcg gtccggtcag
    15061 cagcgtgacc tggcggttgt tcttgtacgt ggagccgtag tagctgctga ggttgtaggc
    15121 gggctcgctg ccaccggtca cgccccacat gccgtcgagc tggttgtaag gaacgtcggg
    15181 ccgcagcact tttgtcaggt tcgacgcagc aggctgcgcg acctcgtaac cgggcaggtt
    15241 cttccacgtg ccggcct
//
LOCUS       DATLBZ010000443         5636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5027345, whole genome shotgun sequence.
ACCESSION   DATLBZ010000443 DATLBZ010000000
VERSION     DATLBZ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5027345"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VMZ53_22410"
     CDS             <1..823
                     /locus_tag="VMZ53_22410"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HUS31288.1"
                     /translation="GMTLDIGGEPDHVEITAGDRVLGNADDAGALTAQFGSLGPLTLT
                     ATAFDKAGMPAATATVDVTIDDPQVADCKAWLDLYKLDYTSGPTNLGITDPVTVTLPL
                     NGVAYRYSGNTTDRKTLYGDCTLMKSLAQAAPILRAHDVTKLVDIGIYNYRCIDQSLT
                     PPNCSMSQHSYAKAIDIAELVTSADVHYSVLKDWVIDSGGTTCAAATENEKDAFLHQV
                     ICELKTAMVWNIVLTPNYNSDHRNHFHVDLTKNSDFIKRTSSELVEPIVDGAFAD"
     gene            complement(833..1486)
                     /locus_tag="VMZ53_22415"
     CDS             complement(833..1486)
                     /locus_tag="VMZ53_22415"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HUS31289.1"
                     /translation="MFLVIGVAAGALTGVPIGPVNVAVIDAAYRHTYRRAMAVGFGGA
                     LADALYAALGVMGVTPFLRTYPTVLTVIYAVSGIVLMVYGWLTARSQPVAPAAPTNPD
                     DHAPTEDSVVFRRRELKQGFTLGIALILLNPAAVVTWVVIMGSVIPDTSTHWDGLACA
                     VGVFVGSFAWFSLVAYLTQKGKHVLGDKAAWIPRVVGVALMIYAVYLLAKAVKFVVA"
     gene            1577..2332
                     /locus_tag="VMZ53_22420"
     CDS             1577..2332
                     /locus_tag="VMZ53_22420"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS31290.1"
                     /translation="MTWLGHATAVAQLGKARVIFDPLLPWRARAAGKVDAILVTHSHV
                     DHLNRWSLKAIDRDTHLVVPRGCKHIVADLGFRAVTEMTAGDQLEVGGLQIEAVETRH
                     DQGRWRKSTGPECVGYVVGGGGAHVHHSGDVDFSDHQVFDDIGKRFAIDATLLPIGGM
                     MPVWYYRMRRANHDQGVHIDPDCALDIYERLGAKRLVPVHWGTLHLRLGLPSMPMKRL
                     IKIAGARNAAGVQVLRHGETLELAGLDADAVKP"
     gene            complement(2306..2536)
                     /locus_tag="VMZ53_22425"
     CDS             complement(2306..2536)
                     /locus_tag="VMZ53_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31291.1"
                     /translation="MNDQILSPDGFKWFLTLIVGIVAAAWFVYDAINLLRTRHADRKD
                     PVVRDKHFGYVMGMLIGIVGVYGCLRFHGIGV"
     gene            complement(2533..3087)
                     /locus_tag="VMZ53_22430"
     CDS             complement(2533..3087)
                     /locus_tag="VMZ53_22430"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUS31292.1"
                     /translation="MAVATAPRVVSPYNPANAVTASRFLCLPPLLWAIDHGYHQWATL
                     FIMICGLLDKVDGLVARIFDCRSAFGELFDAITDGICYGFGLIVVAAYGWAPVLPVVA
                     IIFLGLANSAMRFVYVKRAGRTTNYKSYAMERLVAYTGYLIGFATGGFQVTYFYWVFV
                     PLMLVIVGYDTKRMLIDPIPEPKA"
     gene            complement(3094..3774)
                     /locus_tag="VMZ53_22435"
     CDS             complement(3094..3774)
                     /locus_tag="VMZ53_22435"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUS31293.1"
                     /translation="MPNLFAFRKNLDRVIHPLVSAVAKLPIHANAWTMLGALLGLIGG
                     VLFFYAYWWIGFVVLVVRGLVDHIDGYKARNFNQRSTFGAVMDDVCDRWVLGIMFAGG
                     CLNLSDDYPHILIILGFGITGALTNVIIKLSIYAESQQDIWREKGKIGHPVDVVGLFG
                     SAEFLIYYGSGVFFTALLHDPRPLLAGCWAVAIMSHVSLLQRVRFAWKRYRFVDPGLA
                     DDDQAQAG"
     gene            complement(3792..5090)
                     /locus_tag="VMZ53_22440"
     CDS             complement(3792..5090)
                     /locus_tag="VMZ53_22440"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HUS31294.1"
                     /translation="MTDDLTTAESIEAADQKYVMRPWTHPKGEPVIVAKAKGCTVTDV
                     HGKEYLDLTAGYFVNNAGHCHPRIAQAAMKQMGEVLQVSGKHGTIPAIKLAKKLIELA
                     PKSLHKVFFSTGGSEANEFALKAARQYTKKSDVAYLENGYHGLTLGSLEVTASEKYRE
                     SAGKPMGRENYAIPTSYAYRCKHGPHAECTEKCLDGVEALFDAHPNTAALIAEPVQAV
                     GGLAPSKAWWKKLDEIRKKRGILLIADEVQTGLGRTGKMFAVEHYGLQPDIMTVGKGL
                     SGGVGSMAATIASDEISKAFFGGTTPTSGGNAVSAAAGLELIKILEDEKLVENAAAVG
                     RYFTEAARALKDPWIGDVRFTGLLGGIELVKDTGSREILGKTEMAKIKDLLHEAGMLI
                     TVSGWHGNVLRLQPPLSFTNAMVDQFIAALRKTLQTVRGS"
     gene            complement(5124..>5636)
                     /locus_tag="VMZ53_22445"
     CDS             complement(5124..>5636)
                     /locus_tag="VMZ53_22445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUS31295.1"
                     /translation="WPLVRVRGELAIIGLSTSRQTPWFTAYGRVGAGQLARFEKALGD
                     PRLAGKARLVAIHHPPAGKRAANKIRGLKDHAAFADVIGRVGCDLIVHGHEHQDLTET
                     LTGPSGPIQVRGIASGTYHHNKPDRVARYRVFQIEGGKIVSDFVRVWTREHGMFEAQP
                     GSGQQSNYVS"
BASE COUNT         1077 a         1788 c         1819 g          952 t
ORIGIN      
        1 cggcatgacg ctcgacatcg gcggcgagcc cgatcacgtc gagatcaccg cgggcgaccg
       61 cgtgctcggc aacgcggacg acgccggtgc gctgaccgcg caattcggat cgctcggccc
      121 gctcacgctg accgcgacag cattcgacaa agcgggcatg ccggccgcca cggcaaccgt
      181 cgacgtcacg atcgacgatc cgcaggtcgc cgactgcaag gcatggctcg atctctacaa
      241 gctcgactac acgagcggtc ccacaaacct cggcatcacc gatccggtga cggtcaccct
      301 gcctctcaac ggcgttgcct accgctacag cggcaacacg accgatcgca agacgctcta
      361 cggcgactgc acgctgatga agtcgctggc acaagcggcg ccgatcctgc gcgcacacga
      421 cgtcacgaag ctcgtcgaca tcggcatcta caactatcgc tgcatcgacc aatcactcac
      481 acctccgaat tgctcgatgt cgcagcactc gtatgcaaag gcgatcgaca tcgccgagct
      541 cgtcacgagc gccgacgttc actacagcgt gctcaaagac tgggtcatcg acagcggtgg
      601 gacgacgtgc gcggccgcga ccgaaaacga aaaagacgct ttcctgcacc aagtcatctg
      661 cgagctgaag accgcgatgg tgtggaacat cgtgctgacg ccgaactaca actcggatca
      721 ccgcaatcac ttccacgtcg acctgacgaa gaattctgac ttcatcaagc gaacgtcgtc
      781 agagctcgtc gagccgatcg tagatggcgc tttcgccgac taagcgtgcg agctacgcga
      841 cgacgaactt cacggctttg gcgagcaagt acacggcgta gatcatcagc gcgacgccaa
      901 cgacgcgcgg gatccacgcg gccttgtcgc cgagcacgtg cttgcccttc tgcgtgaggt
      961 acgcgacgag cgagaaccag gcaaagcttc cgacgaacac accgaccgcg cacgcgaggc
     1021 cgtcccagtg cgtcgacgtg tccggaatca ccgagcccat gatcacgacc cacgtcacga
     1081 ccgccgcggg gtttagcagg atcagcgcga tgccgagcgt gaagccttgc ttgagctcgc
     1141 ggcggcggaa cacgacggag tcttcggtcg gcgcgtgatc gtcgggattc gtcggcgcag
     1201 cgggcgcgac gggttggctg cgagcggtga gccagccgta gaccatcagc acgatgccgc
     1261 tgaccgcgta gatgacggtg agcaccgttg gatacgtgcg gaggaatggc gtcacgccca
     1321 tcacgccgag cgcagcgtag agcgcgtccg cgagcgcacc accgaagccg accgccatcg
     1381 cgcggcgata cgtgtgtcga taggcagcgt cgatcaccgc gacattcacc gggccgatag
     1441 gcacgcctgt gagtgcgccg gcggcaacgc cgatcacgag gaacatcagc acgggggagc
     1501 ccccgtgcta tacagcaccg tgaggtcgcc tgctatgccg aacatagggt cttcctccga
     1561 ggaaacacac ccttcggtga cgtggctcgg ccatgcgacc gcggtggccc agctcggaaa
     1621 agctcgggtg atcttcgatc cgcttctgcc gtggcgcgcg cgtgccgcgg gcaaggtcga
     1681 cgcgattctg gtgacgcact cgcacgtcga tcacctcaat cgctggagcc tcaaggccat
     1741 cgatcgagac acgcacctcg tcgtgccgcg cggctgcaag cacatcgtcg cggacctggg
     1801 atttcgcgca gtcaccgaga tgaccgcggg cgatcagctc gaggtcggcg gcctgcagat
     1861 cgaggccgtc gagacgcgtc acgatcaggg acgctggcgc aagtcgactg ggcccgagtg
     1921 cgtcgggtat gtcgtcggtg gcggcggcgc gcatgtccat cactcgggag acgtcgactt
     1981 ctcggatcac caggtcttcg acgacatcgg caagagattc gccatcgatg cgacgctgtt
     2041 gccaatcggc gggatgatgc ccgtctggta ttaccggatg cgccgcgcaa atcacgatca
     2101 aggcgtgcac atcgatcccg attgcgcgct cgacatctac gagcggctcg gcgcgaagcg
     2161 cctcgtgccc gtgcactggg gcacgctgca tctgcggctc ggtttgccgt cgatgccgat
     2221 gaagcggctc atcaagatcg cgggcgcgcg caatgccgcc ggcgtgcagg tactgcggca
     2281 cggcgaaacg ctcgagctcg ctgggctaga cgccgatgcc gtgaaaccgt aggcagccgt
     2341 agacgccgac aattccgatc agcatgccca tgacgtagcc gaagtgcttg tcgcgcacga
     2401 ccgggtcttt gcgatcggcg tggcgggtgc gcagcaagtt gatcgcgtcg tagacaaacc
     2461 acgctgccgc gacgatgccc acgatcagcg tcaggaacca cttgaagccg tcgggcgaca
     2521 ggatctgatc gttcatgctt tcggctccgg gatgggatcg atcagcatcc gcttcgtgtc
     2581 gtagccgacg atgacgagca tcagcggcac gaacacccag tagaagtacg tgacctggaa
     2641 gccgccggtt gcaaagccga tgaggtagcc cgtgtacgcg acgagacgct ccatcgcgta
     2701 ggacttgtag ttcgtcgtgc ggccggcgcg cttgacgtag acgaagcgca tcgcggagtt
     2761 cgcgagaccg aggaagatga tggcgacgac gggaagcacg ggcgcccagc cgtacgccgc
     2821 gacgacgatg aggccaaagc cgtagcagat gccgtcggtg atcgcgtcga acagctcacc
     2881 gaacgccgag cggcaatcga agatgcgcgc gaccaagccg tcgaccttgt cgagcaggcc
     2941 gcagatcatg atgaagagtg tcgcccactg gtgatagccg tggtcgatcg cccagagcag
     3001 cggcggcaag cacaggaagc gcgaggctgt gacggcgttt gccgggttgt acggcgacac
     3061 gacgcgtgga gcagttgcga cagccacgag cgactaccct gcttgcgctt gatcgtcgtc
     3121 ggcgaggccc gggtcgacga accgatagcg cttccacgca aagcggacgc gctgcaacag
     3181 agagacgtgc gacatgatcg cgacagccca gcagccggcg agcagcggac gcggatcgtg
     3241 caagagcgcg gtgaagaaca cgcccgagcc gtaatagatg aggaactcgg ccgagccgaa
     3301 gaggccaacg acgtcgacgg ggtggccgat cttccccttc tcgcgccaga tgtcttgttg
     3361 gctctcggcg tagatcgaca gcttgatgat cacgttcgtc agcgcgcccg tgatgccgaa
     3421 cccgaggatg atcaggatgt gcgggtaatc gtcgctgagg ttgaggcagc cgccggcgaa
     3481 catgatgccg agcacccagc ggtcgcagac gtcgtccatc acggcgccga acgtcgagcg
     3541 ttggttgaag ttgcgcgcct tgtagccatc gatgtgatcg acgaggccgc gaacgacgag
     3601 gaccacgaag ccgatccacc agtacgcgta gaagaacagc acgccgccga tgaggccgag
     3661 cagagcgccg agcatcgtcc acgcgtttgc gtggatcggg agcttcgcca ccgcagacac
     3721 gagcgggtgg atgacccgat cgaggttctt acgaaaggcg aacaggtttg gcatgcgagg
     3781 gggaaactct ctcagcttcc gcggacggtc tgcaaggtct tacgaagcgc agcgatgaac
     3841 tgatcgacca tggcgttcgt aaaggagaga ggcggctgca ggcgcagcac gttgccatgc
     3901 cagcccgaga ccgtgatgag catacccgcc tcgtgcagga gatctttgat cttggccatc
     3961 tctgtcttgc cgaggatctc gcgcgagccc gtgtccttga ccagctcgat accgccgagg
     4021 aggcccgtga accggacgtc gccgatccat ggatccttca gcgcgcgcgc agcctcggtg
     4081 aagtagcggc cgacggcagc cgcattctcg acgagcttct catcctcgag gatcttgatc
     4141 agctcgaggc ccgcggcggc cgagacggcg ttgccgccgg acgtgggcgt ggtgccgccg
     4201 aagaacgcct tgctgatctc gtcgctcgcg atcgtcgcgg ccatcgagcc gacgccgccg
     4261 gagagaccct tgccgacagt catgatgtcg ggctgcaggc cgtagtgctc gacggcgaac
     4321 atcttgccgg tccgaccaag gcccgtctgc acttcgtcgg cgatgagcag aatcccgcgc
     4381 ttcttgcgga tctcgtcgag cttcttccac cacgccttcg acggtgcgag accgccgacg
     4441 gcttgcacgg gctcggcgat cagcgcggca gtattgggat gcgcgtcgaa cagcgcctcg
     4501 acgccgtcga gacacttctc ggtgcattcg gcatgcgggc cgtgcttgca gcggtaggcg
     4561 tacgacgtcg ggatcgcata gttctcgcga cccatcggct tgccggcgct ctcgcgatac
     4621 ttctcgctcg cggtgacctc gagcgaaccg agcgtcagtc cgtgatagcc attctctaga
     4681 tacgcgacgt cgctcttctt cgtgtactgg cgcgcagcct tgagcgcgaa ctcgttcgcc
     4741 tcgctgccgc ccgtcgagaa gaacaccttg tgcagcgact tcggcgcgag ctcgatgagc
     4801 ttcttcgcga gcttgatcgc cgggatggtg ccgtgcttac cggagacctg cagcacctcg
     4861 cccatctgct tcatcgcggc ttgggcgatc cgcggatggc agtgaccggc attgttcacg
     4921 aagtagcccg ccgtcaggtc gagatactcc ttgccgtgaa cgtcagtaac cgtgcagccc
     4981 ttcgccttgg cgacgatgac gggctccccc tttgggtgcg tccacggacg catcacgtac
     5041 ttttgatcag cggcttcgat ggactcggcg gtcgtgaggt cgtcggtcac ttgggctgag
     5101 gtatctcatg cctcaaatca ttgtcaactt acatagttag attgctgacc gctgccaggc
     5161 tgtgcctcga acatgccgtg ttctcgcgtc cagacgcgta cgaaatcgct gacgatcttg
     5221 ccgccctcga tctggaatac gcgatagcgc gcgacgcgat cgggcttgtt gtgatgatag
     5281 gtgcccgacg caattccgcg gacctggatc ggtccggatg gccctgtgag ggtctcggtc
     5341 aggtcctggt gttcatgtcc atggacgatc agatcgcagc cgacgcgacc gatcacgtcc
     5401 gcgaacgctg catggtcctt gagcccgcgg atcttgttcg ccgcgcgctt gcccgcgggc
     5461 ggatgatgga tggcaacgag gcgagcttta cccgcgagcc gcggatcgcc gagcgccttc
     5521 tcgaagcgtg cgagctggcc ggcgccgacg cgtccatacg cggtgaacca cggcgtctgc
     5581 ctcgaggtcg agaggccgat gatcgcgagc tcgccgcgca cgcgaacgag gggcca
//
LOCUS       DATLBZ010000444         2800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3272639, whole genome shotgun sequence.
ACCESSION   DATLBZ010000444 DATLBZ010000000
VERSION     DATLBZ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272639"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VMZ53_22450"
     CDS             <1..241
                     /locus_tag="VMZ53_22450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31296.1"
                     /translation="VVTLNWSVAKYLRAVGGVALCMLAAGGVAAIVYPLLPAGHGVRA
                     LVTTVVVVVVSALLLAYTQGLTLRGVVRSMREPPP"
     gene            complement(238..1023)
                     /locus_tag="VMZ53_22455"
     CDS             complement(238..1023)
                     /locus_tag="VMZ53_22455"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HUS31297.1"
                     /translation="MQPFWYRTFPRATFVARRLVGASYEREMQLLDVLCDRTKLGIDI
                     GAKVGMYTYRIRAHSAEVLAFEPIPLFHNMLKRVFEGKRGRIEPYAVSNKRGSAVLRM
                     PYDQTGGAQFGRSTIDVGNKLEHQVVASVRELEVETRTLDDYDFDAVGFIKIDVEGHE
                     MAVLEGAEKTIARHHPNLLVECNDDHSADGVAKLTTWMQERGYSLYFLHHRVIRPVAE
                     YDRSVHWKDHTIENFICVHESRPDVVKALHERAARTSLRRAAV"
     gene            complement(1023..1754)
                     /locus_tag="VMZ53_22460"
     CDS             complement(1023..1754)
                     /locus_tag="VMZ53_22460"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HUS31298.1"
                     /translation="MIRNAVILAAGMGTRLRGEGDLPKPLFEVGGVALIKRTILTLAD
                     AGVTRVVVVTGFMAERIRGAVADDPTYAQRGITIEFAHNAEYTLKNGISVLVGGKQVG
                     GPFMLSMADHIYTPAVARIVGSSDMSVADLYLATDRRITEVLDIDDATKVRSEGGRIV
                     DIGKTIPIYDRIDCGVFAVTPRLLEALEAVRAELGDCSLSEGVKRLAQAGAARVADIG
                     EETWQDVDTPADRAHVEGLLAKRGS"
     gene            complement(1802..>2800)
                     /locus_tag="VMZ53_22465"
     CDS             complement(1802..>2800)
                     /locus_tag="VMZ53_22465"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HUS31299.1"
                     /translation="SCDSQPCRLQFAAWLCSILVQSVAKTLGVRLHDARRMAHEALHH
                     FLRRILSKYPIRFGAAAINRILDGAFGGSDANWLVAPSRGYFPTMVLDVSTNLQRKLF
                     YFPRVYGRFYGTNTFAKFIRGRLKPGDTFYDIGANVGFFSLLAAGIVGDSGSVYAFEP
                     EPVTCDSIAKSAKVNGYSRLHAVQCALSDHEGEADFYCARDGTANSLVTEAPGREKRY
                     ERKVTTRVTRLDNLVAEGTIDPRGLSLIKVDVEGAEARTVRGMRESLVAADYPAIWCE
                     VRGPKGSTRAPNTYPAVLSVLKDMGYTPVIWSEQGRRHVRDEDVVGRADVLFERSR"
BASE COUNT          477 a          903 c          901 g          519 t
ORIGIN      
        1 ggtcgtgacc ctcaactggt cggtcgcgaa gtacctgcgt gcggtgggcg gcgtggccct
       61 gtgcatgctc gcggcgggcg gcgtggcagc tatcgtctat ccgctgcttc ccgcgggtca
      121 tggcgtgcgg gcgctcgtca cgacggtcgt cgtcgtggtg gtcagtgcat tgctgctcgc
      181 ctatacgcag gggctcacgt tgcgcggcgt agtgcggtcg atgcgcgaac cgccgcctta
      241 aaccgcggcg cgacgcaggc tcgtgcgagc ggcacgttcg tgcagcgcct tcacgacgtc
      301 gggtcgcgat tcgtgcacac agatgaagtt ctcgatcgtg tgatctttcc agtgcacgct
      361 gcggtcgtac tctgcgacgg ggcgaatcac gcggtggtgc aggaaataga gcgaataacc
      421 gcgctcctgc atccaggtcg tcagcttcgc cacgccatcc gcgctgtggt catcgttgca
      481 ttcgacgagc aggttcggat ggtgacgcgc gatcgtcttc tccgctcctt cgaggaccgc
      541 catctcgtgg ccctcgacat cgatcttgat gaagccgacg gcgtcgaagt cgtaatcgtc
      601 gagggtgcgg gtctcgacct cgagctctcg cacgctcgcg accacttgat gctcgagctt
      661 gtttccgacg tcgatcgtcg agcgtccgaa ctgcgcgccg ccggtttggt cgtacggcat
      721 ccgcagcacc gcgctgccgc gcttgttcga caccgcgtac ggctcgatgc ggccgcgctt
      781 gccttcgaag acgcgcttga gcatgttgtg gaatagcgga atcggttcga acgccagcac
      841 ctccgccgag tgggcgcgga tccggtacgt gtacatcccg actttcgcgc cgatgtcgat
      901 gccgagcttg gtgcgatcgc acagcacgtc gaggagctgc atctcgcgct cgtagctcgc
      961 accgaccagg cgcctcgcga cgaacgtcgc gcgcggaaag gtccgatacc agaacggctg
     1021 catcagcttc ctctctttgc gagcaagcct tcgacgtgcg cacgatcggc cggggtgtcc
     1081 acgtcctgcc acgtctcttc accaatgtcg gcgacgcgcg ctgcgccagc ttgtgcgagc
     1141 cgcttcacgc cttcggacag cgagcagtcg ccgagctcgg cgcgtaccgc ttcgagagcc
     1201 tccagcaggc gcggcgtcac ggcgaacaca ccgcagtcga tgcggtcgta gatcgggatc
     1261 gtcttgccga tgtcgacgat gcgaccgcct tcgctgcgca ccttcgtcgc gtcgtcgata
     1321 tcgaggacct cggtgatgcg ccgatcggtc gcaaggtaga ggtcagcgac gctcatgtcc
     1381 gacgagccga cgatgcgcgc gactgcgggc gtatagatgt ggtcggccat cgacagcatg
     1441 aagggcccgc cgacctgttt gcctccgacg aggacggaga tcccgttctt gagcgtgtac
     1501 tccgcgttgt gcgcgaattc gatcgtgatg ccacgctgcg cataggtcgg atcgtcggca
     1561 accgcgccgc gaatgcgctc ggccatgaac ccggtgacca ccaccacgcg cgtcacgcct
     1621 gcatcggcga gcgtgaggat ggtgcgcttg atcagcgcaa cgcctccgac ctcgaagagc
     1681 ggcttgggca gatcgccttc tccgcgcagg cgcgtaccca tgccagcggc aaggatgacg
     1741 gcgttccgga tcatgggggc cgaccttacg cgaccgagct agcgagtcaa ggcaaacgca
     1801 tctatcgcga gcgttcgaac agtacgtctg cgcggccgac gacgtcctca tcccgaacgt
     1861 ggcgtcgtcc ctgctcggac cagatcacgg gcgtgtaccc catgtccttc agcacggaca
     1921 gcacggctgg gtacgtattg ggcgcccgcg tggagccttt agggccgcgc acttcgcacc
     1981 agatcgcagg gtaatcggca gcgaccaagg attcgcgcat gccgcgaacc gtgcgcgcct
     2041 ctgcaccctc tacgtccact ttgatcagcg agaggccgcg cggatcgatc gtgccctcgg
     2101 ccacgaggtt gtcgagcctc gtcacacgcg tcgtcacctt gcgctcgtac cgcttctcgc
     2161 gacccggcgc ctccgtgaca agggagttcg cggtgccgtc acgcgcacag tagaagtcgg
     2221 cttcgccctc gtgatccgag agtgcgcatt gcacagcatg aaggcgtgag tagccgttga
     2281 ccttggcgct cttcgcaatc gagtcgcacg ttaccggctc tggttcgaac gcgtacacac
     2341 tgccgctatc accgacgatg cccgccgcga ggagcgagaa gaagccgacg ttcgcgccga
     2401 tgtcgtagaa cgtgtcgccc ggcttgagtc tgccccggat gaattttgcg aacgtgttcg
     2461 tgccgtagaa gcgaccgtag acgcgcggga agtagaagag cttgcgctgc aaattcgtcg
     2521 agacgtcgag gaccatcgtc gggaagtagc cgcggctcgg cgcgacaagc caattcgcat
     2581 cgctaccgcc aaaagcgcca tcgaggatgc gattgatcgc agcggcgccg aacctgatcg
     2641 gatacttcga gaggatccgg cgtagaaaat ggtgcagagc ctcgtgtgcc atccgccgcg
     2701 catcgtgcaa gcgcacgccc agcgtcttcg cgacggattg cacgaggata gagcagagcc
     2761 atgctgcaaa ttgcagccga cacggctgcg aatcgcagct
//
LOCUS       DATLBZ010000445         6292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2795506, whole genome shotgun sequence.
ACCESSION   DATLBZ010000445 DATLBZ010000000
VERSION     DATLBZ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2795506"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VMZ53_22470"
     CDS             <1..369
                     /locus_tag="VMZ53_22470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31300.1"
                     /translation="GAYTFDPASLKGKPSIVMFVTPTCSHCLATIPRAMAAAKAEQAN
                     VVAVFIAGGKQNAEGVISHTKFEGPALVDDGTLRRKYNIKGVPYILVLGPDGHARNAY
                     RGEHGDDDVQTFREALADAK"
     gene            complement(391..960)
                     /locus_tag="VMZ53_22475"
     CDS             complement(391..960)
                     /locus_tag="VMZ53_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31301.1"
                     /translation="MRRALLMIMLATPALFAHNVGAQGSAQEPKGQNPEGEYGGVQPG
                     QPKKAEPAKKTKKPPPKGTLSWIGFETKDGGSNVFFQSVAPFEVSQRVENGTLVVGLS
                     LSKLGQNTWRPIDTRFFETSVARVSAKKKGKGIEVRIAFKDAKNAAQGSLRTATEADG
                     MYYAYLSFAGGGSTTGTTGSADAPDAPEK"
     gene            1098..1661
                     /locus_tag="VMZ53_22480"
     CDS             1098..1661
                     /locus_tag="VMZ53_22480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31302.1"
                     /translation="MRSVKGSLFLDYVRMLKAHKGVEWSAFLSPEDLAYLSMTIDKTA
                     WYPMESFERMGNAILKFVAGGELMPVQLWGRYSSAQMKHAHPKLLAPGDPVETLNRFK
                     VMRSTFFDFEALQVLMLHDGEAQISIAYHMGMPAEEAASVQTMGFFEGLLELAGAKEI
                     RAKFREKSWAGDARTVLDIRWVSPLSR"
     gene            complement(1683..3122)
                     /locus_tag="VMZ53_22485"
     CDS             complement(1683..3122)
                     /locus_tag="VMZ53_22485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HUS31303.1"
                     /translation="MAADASAYRDLLTRLLGARRFGMKLGLDRIRALLDQLGAPDRRL
                     GTVVHIAGTNGKGSTVAMLATLVRGCGKRVATYTSPHLSTLRERITIDGQLVSEQAIV
                     EAAARVEAAGGGELTFFEQITAIAMLIIADARVDVTILEVGLGGRLDATNVVDARTAP
                     IAVITGVAMDHEAILGTTLDAIAREKAGIVKRGQRVVIGASGEPAAVPLLRELAFAAG
                     AARVTVVDDSAIARVPPVALPGAHQRANAAAALAALDDLEMAPAPPHDIVELLAHVTH
                     PGRFELVGATTIADIADNRDAVILDGAHNPHGARALAATLRAHRLRPVLVVAVSADKD
                     VRAIADALAPEVSAVIATRYQQERALDPPTLADAFRAAIERMNVHSPNRPQIDFLDDR
                     DATSRASSVRDGRDATSGSSSIRDGRGATSVRVELAADLEAALARARVLGGPILVAGS
                     LFIVGEARVLLLGAPADPIAISDPAKPAT"
     gene            complement(3126..4031)
                     /gene="accD"
                     /locus_tag="VMZ53_22490"
     CDS             complement(3126..4031)
                     /gene="accD"
                     /locus_tag="VMZ53_22490"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HUS31304.1"
                     /translation="MAWWSRTQGLAEQPKKSVPKGIWTKCERCSTTIYEADLDANLRV
                     CSSCNHHFRVPTEARIAMMVDPGSWQEHDRTLESGDPLGFKVDNKKYVDQIKGTTKKV
                     GAGDAYRAGTCTVGGIPSEVGFFVFEFMGGSMGSVVGEKITRQFERAVAHKRPAILFS
                     ASGGARMQEGVLSLMQMAKTSAARSKLREARVPYIAVLLHPTTGGVAASVAMLGDLII
                     AEPDALIGFAGPRVIEQTIRQQLPPGFQRSEFLLEHGMVDLVVERKDLKPTLERTLRW
                     FERGAVPERRTGTVFDETADIPATD"
     gene            4176..4487
                     /locus_tag="VMZ53_22495"
     CDS             4176..4487
                     /locus_tag="VMZ53_22495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31305.1"
                     /translation="MSSSVLVVNEISTEREEIARALEAEGFTVVQASSAAEAVREIWG
                     GSFIVAVVSTLLTGTTSTALVQQLGQMAPEIETVVHGKNDELPALVRKCIQIREGEAA
                     A"
     gene            complement(4484..5158)
                     /locus_tag="VMZ53_22500"
     CDS             complement(4484..5158)
                     /locus_tag="VMZ53_22500"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HUS31306.1"
                     /translation="MADGLDRFARDFKAGTVLFEEGQPGDYMYVITRGEVEIRRKVGE
                     TERVLAVLPAGEFFGEMAILNARPRSATAVVRIDSRLLVIEGSTFEAMLRARPEIALR
                     IIKALAVRLENANQHVELLLLPTPNHRVVQCLRHMAEEQMLLAGGQIREGTAILVPKR
                     VEDIAVRVGLPVHEVIDVVDRLRAARLVLMAEDAGIAGDGFIVPEVGRLQEFLEFLNL
                     KDRFGA"
     gene            complement(5169..5819)
                     /locus_tag="VMZ53_22505"
     CDS             complement(5169..5819)
                     /locus_tag="VMZ53_22505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31307.1"
                     /translation="MPRVPPSPVIDVVVPVRDHAASLGALLRDLPHRSVRSVVVVDRD
                     SKDRSAEIARDAGALVLREASGGYGAACLRAQAHFGALPRVPEVIVFIPGDRPAAASA
                     LLDLVAPIGERGVELVLGVETRKRRVREKLALGLIGTVYGQRWSGVGPVRALKFPALV
                     ALGMRDHSDGWDVEMLVRAVKLGLTCDEVPLPGTDEKAPKIGSRALLHILRHSTMR"
     gene            5835..>6292
                     /locus_tag="VMZ53_22510"
     CDS             5835..>6292
                     /locus_tag="VMZ53_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HUS31308.1"
                     /translation="MALTYKDAGVDIDAGNALVERIKPLAKATTRPEVLGGIGGFAAL
                     CRIPQQYKEPILVSGTDGVGTKLKTAFAANRHDTIGIDLVAMCVNDVLVTGAEPLFFL
                     DYFGTGSLDVETGASVIGGIAEGCKQAGCALVGGETAELPGLYHGKDYDLA"
BASE COUNT         1066 a         2081 c         2074 g         1071 t
ORIGIN      
        1 ggcgcgtaca cgttcgatcc tgcgtcgctg aagggcaagc cgagcatcgt catgttcgtc
       61 actccgacgt gctcgcactg cctcgcgacg atccctcgcg cgatggctgc tgcgaaggcg
      121 gagcaagcga acgtcgttgc ggtgtttatc gcgggcggca agcaaaacgc cgagggcgtg
      181 atcagtcaca cgaagttcga gggccccgca ctcgtcgacg acggcacact ccgccgcaag
      241 tacaacatca agggcgtgcc gtacatcctc gttctcggcc ccgacggtca cgcgcgcaac
      301 gcctaccgcg gcgagcacgg cgatgatgac gtgcagacgt tccgcgaagc gctcgccgac
      361 gcgaagtaat cgcgcgaggc ggaacttgcc ctacttctcg ggcgcgtcag gagcgtcggc
      421 gctgccggtc gttccggtcg tgctgccacc acccgcgaac gacaggtacg cgtagtacat
      481 gccatcggct tcggtcgccg tgcggagcga gccctgcgcc gcgttcttcg cgtctttgaa
      541 cgcgatgcga acctcgatgc ctttgccctt cttcttggcg ctcacgcgcg cgacggatgt
      601 ctcgaagaag cgcgtgtcga tcgggcgcca cgtattctgg ccgagcttcg agagcgacag
      661 cccgacgacg agcgtgccgt tctcgacccg ctggctcact tcgaacggcg ctaccgactg
      721 gaagaacacg ttcgagccgc catctttggt ctcgaagccg atccacgaca gcgtgccctt
      781 cggcggcggc ttcttcgtct tcttcgccgg ctcggccttc ttgggctgcc ccggctgaac
      841 gccgccgtac tcgccttcgg gattctgccc cttcggctct tgcgcagatc cttgggctcc
      901 cacgttgtga gcgaacaagg cgggggtcgc caacatgatc atcaagagcg cgcgacgcat
      961 aaatagatcg taggtggatg cagcgagccg cgcaaattcg gtctcggacg tgtgccgagt
     1021 tacgtaagta cttcgcaaat aaccggcgtg tacccggcgc tgctcgttag agagcgcaaa
     1081 cggggccatg atgcggcgtg cgcagtgtca aaggcagttt gtttctcgac tacgtgcgca
     1141 tgttgaaggc tcacaagggt gtcgagtgga gcgcgttcct ctcgcccgag gacctcgcgt
     1201 atctgtcgat gacgatcgac aagaccgctt ggtatccgat ggagtcgttc gagcgcatgg
     1261 gtaacgcgat cttgaagttc gtcgcgggtg gcgagctgat gcccgtgcag ctgtggggac
     1321 gttactcgtc ggcgcagatg aagcacgcgc atccgaagct cctggctccg ggcgatccgg
     1381 tcgagacgct caaccgcttc aaagtcatgc gctcgacgtt cttcgacttc gaagcgctgc
     1441 aggtgctcat gctccacgac ggcgaggcgc aaatctcgat cgcctatcac atggggatgc
     1501 ccgccgaaga agccgcctcc gtccagacga tggggttctt cgagggcctg ctcgagctcg
     1561 cgggtgccaa agagattcgc gcgaagtttc gcgagaagtc gtgggccggc gacgcgcgca
     1621 ccgtcctcga tattcgctgg gtctcgccgc tgagccgcta gctcgctggc gtcgccggat
     1681 cgctacgtcg ctggcttcgc aggatcactg atcgcgatcg gatcggccgg cgcaccgagc
     1741 aagagcaccc tcgcctcgcc gacgataaag agcgacccgg caacgaggat cggaccacca
     1801 agaacgcgcg cccgcgccag cgccgcctcg agatccgcag ccagctcgac gcgaacgctc
     1861 gtcgcacctc ggccatcgcg aatgctcgaa gagccgctcg tcgcatcgcg gccatcgcga
     1921 acgctcgacg ccctgctcgt cgcatcgcgg tcgtcgagga aatcgatctg cgggcgattg
     1981 ggtgaatgaa cgttcattcg ttcgatcgcg gcgcggaatg catcggcgag cgtcggcgga
     2041 tcgagcgcgc gctcttgctg gtagcgcgtc gcgatcaccg cggacacttc gggcgcgagt
     2101 gcgtcggcga tcgcgcgaac gtctttgtcg gcactcaccg cgacgacgag cacgggccgc
     2161 aggcgatgag cgcgaagcgt tgcggcaagc gcgcgtgcgc cgtgcgggtt gtgcgcaccg
     2221 tcgaggatca ccgcatcgcg gttgtccgcg atgtccgcga tggtcgttgc gccgacgagc
     2281 tcgaaccggc ctggatgggt gacatgcgcg agcagctcga cgatgtcgtg cggcggcgcg
     2341 ggcgccatct cgagatcgtc gagcgcggcg agcgctgccg ctgcattcgc gcgttgatga
     2401 gcgccgggaa gcgcgaccgg cgggacgcgc gcgatcgccg aatcgtcgac gacggtcacg
     2461 cgtgcggcac ctgcggcgaa tgcgagctct cgcaagagcg gcacggcagc gggctcgccc
     2521 gacgcgccga tcacgacgcg ctggccgcgt ttgacgatgc cggccttctc gcgcgcgatc
     2581 gcgtcgagcg tcgtcccgag gatcgcctcg tgatccatcg cgacgcccgt gatcacggcg
     2641 atgggcgcgg tgcgcgcatc gacgacgttt gtcgcgtcga gcctgccgcc gaggccgacc
     2701 tcgaggatgg tcacgtcgac gcgcgcgtcg gcgatgatta gcatcgcgat cgcggtgatc
     2761 tgctcgaaga atgtcagctc gccaccgccg gctgcctcga cgcgtgcggc ggcttcgacg
     2821 atcgcttgct cgctgacgag ctgaccgtcg atcgtgatgc gctcgcgcaa cgtcgagaga
     2881 tgtggcgacg tgtacgtcgc gacgcgcttg ccgcaacctc gcacgagcgt tgcgagcatg
     2941 gcgaccgtcg agcctttgcc gttggtcccc gcgatgtgga cgaccgtgcc gagccgtcgg
     3001 tctggcgcgc cgagctgatc gagcagcgcg cgaatgcgat cgagcccgag cttcatgccg
     3061 aagcgacgcg caccgagcaa gcgcgtcagc agatcgcgat acgcggacgc gtccgctgcc
     3121 acgagctagt ccgtcgccgg gatgtcggcc gtctcgtcga agacggtgcc tgtgcgtcgc
     3181 tcgggaacgg cgccgcgctc gaaccagcgc agcgtgcgct cgagcgtcgg cttgagatct
     3241 ttgcgctcga cgacgaggtc gaccatgccg tgctcgagga gaaactccga gcgctggaag
     3301 ccgggcggca gctgctggcg gatcgtctgc tcgatcacgc ggggaccggc gaagccgatg
     3361 agcgcgtcgg gctctgcaat gatgagatcg ccgagcatcg cgactgacgc ggccacgccg
     3421 cccgtcgtcg ggtgcagcag caccgcgatg tatgggacgc gcgcttcgcg cagcttgctg
     3481 cgggccgcgc tcgtcttcgc catctgcatc agcgagagca cgccctcctg catgcgggcg
     3541 ccgcccgatg ccgagaacag gatcgccggg cgcttgtgcg cgaccgcacg ctcgaactgg
     3601 cgcgtgatct tctcgccgac gaccgagccc atggagccgc ccatgaactc gaacacgaag
     3661 aagccgacct cgctcggaat gccgccgacc gtgcacgtgc ccgcgcgata cgcgtcgccc
     3721 gcgccgacct tcttcgtcgt gcctttgatc tggtcgacgt acttcttgtt gtcgaccttg
     3781 aagccgagcg ggtcaccgct ctccagcgtc cggtcgtgct cttgccaact cccgggatcg
     3841 accatcatcg cgatccgggc ctcggtcggg acccggaagt ggtgattgca gctggagcag
     3901 acccgcaaat tcgcgtcgag atctgcctcg tagatcgttg tagagcaccg ctcacacttc
     3961 gtccaaatcc ctttggggac ggactttttg ggctgctccg cgagcccttg ggtgcgggac
     4021 caccacgcca tgggtcgcac tgataccact cacgccaagt atggtaaagg gcttggattg
     4081 cggtcgtccc cggggtctgg tagatagcaa aggggttacg cgcctgaagg gcgttacgtg
     4141 tttggacccg ccccgctccg tacaaaggtg tcgcgatgtc ttcgtcggtc cttgtcgtta
     4201 atgaaatcag cacggagcgc gaggaaattg cccgcgctct cgaagccgag gggttcacgg
     4261 tagttcaagc gtcttcggcg gcggaagccg tccgcgagat ctggggtggg tcgtttatcg
     4321 tggcggtggt ctcgacgctt ttgaccggaa ccacgagcac ggcgctcgtc cagcagctcg
     4381 gccaaatggc gcccgagatc gagaccgtgg tgcacggcaa gaacgacgag ctgcccgctc
     4441 tcgttcgcaa gtgcatccag atccgcgaag gcgaagcggc agcttaggcg ccgaagcgat
     4501 ccttcaggtt caagaactcc aagaactctt gtagtcgtcc cacttcggga acgatgaagc
     4561 cgtcgcctgc gatgccggcg tcctccgcca tgaggacaag gcgcgcggcg cgcagtcgat
     4621 cgaccacgtc gatcacctcg tgcactggca agccgacgcg gaccgcaatg tcctcgacgc
     4681 gcttgggcac gaggatcgcg gtgccctcgc ggatctgacc accggcgagc agcatctgct
     4741 cttcggccat gtggcgaagg cactgcacga cgcggtggtt cggcgttggc agcagcaaca
     4801 gctcgacgtg ctggttcgca ttctcgagcc gcactgcgag cgctttgatg atgcgcagcg
     4861 cgatctccgg acgcgcacgc agcatcgctt cgaacgtact gccttcgatc acgagcagac
     4921 gcgagtcgat ccgtacgacc gcggtcgcgc tgcgaggccg cgcgttgaga atcgccatct
     4981 cgccgaagaa ctcgccagcc ggcagcactg cgagcacgcg ctcggtctcg ccgaccttgc
     5041 ggcgaatctc tacttcgcca cgggtgatga cgtacatgta atcgccgggc tgtccctcct
     5101 cgaacagcac ggtgcccgcc ttgaagtcgc gagcgaagcg gtccagaccg tcggccacca
     5161 cggaggcatt atctcatggt cgagtgccga agaatgtgca ggagcgcgcg gctcccgatc
     5221 ttcggtgctt tctcgtcggt gccaggcagc gggacctcgt cgcaggtcag accgagcttc
     5281 accgcgcgca cgagcatctc cacgtcccaa ccgtcgctgt ggtcgcgcat gccgagcgcc
     5341 acgagcgcag gaaacttcag cgcgcgcacc ggaccgacgc ccgaccagcg ctggccgtag
     5401 accgtaccga tgaggccgag tgcgagcttc tcgcggacgc gacgcttgcg cgtctccaca
     5461 ccgagcacga gctcgacgcc gcgttcaccg atcggcgcga cgagatcgag cagcgccgat
     5521 gcggcggctg ggcgatcgcc cggtatgaac acgatgactt cgggcacgcg cggcagcgca
     5581 ccgaaatgcg cttgggcacg caagcacgcg gcaccgtagc caccgctggc ctcgcgcagt
     5641 acgagcgcgc ctgcatcgcg tgcaatctcg gcgctgcgat ctttcgaatc gcggtcgacg
     5701 acgacgacgg agcgcaccga ccgatggggc aagtcgcgca gcaacgcacc aagactcgcc
     5761 gcatggtcgc gcacaggcac gacaacgtcg atcacgggcg acggaggcac ccgtggcatg
     5821 ttacacgagc ctcgatggcg ctcacctaca aggacgcagg agtcgatatc gacgccggca
     5881 acgcgctggt cgagcgcatc aagccgctcg cgaaggctac gacccgtccc gaggtgctcg
     5941 gtggcatcgg cgggttcgcc gccctctgcc gcatcccgca gcagtacaag gagccgatcc
     6001 tcgtctccgg taccgatggc gtcggcacga agctcaagac cgcgttcgcc gcgaaccgcc
     6061 acgatacgat cggcatcgat ctcgtcgcga tgtgcgtcaa cgacgtgctc gtcacgggcg
     6121 cggagccgct gttcttcctc gattacttcg gcaccggctc gctcgatgtc gagaccggcg
     6181 catcggtcat cggcggcatc gccgaaggct gcaagcaagc gggctgcgcg ctcgtcggcg
     6241 gcgagaccgc ggagctgccc ggcctttatc acggcaagga ttacgacctc gc
//
LOCUS       DATLBZ010000446        21304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9502321, whole genome shotgun sequence.
ACCESSION   DATLBZ010000446 DATLBZ010000000
VERSION     DATLBZ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21304
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9502321"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1158)
                     /locus_tag="VMZ53_22515"
     CDS             complement(253..1158)
                     /locus_tag="VMZ53_22515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUS31309.1"
                     /translation="MRSLVSIIVVLLAAGAASAAPSTQSNPAFLGIAMVDFAGGCEIT
                     GITRCSPAEDGGLRDSDLVVGMDGNALVDPHTAPQQRRSSCDVLREKIMAHAPGDVAA
                     FELRRNGRPATVKVTLSTRADVQHRCFVGQAVPGLETKDIDNPDHEIDLGELRGKTTV
                     LAWFRLDRCISCGSVIDKVADGIRDRIKDETPDVIGITAHNSDPLQSTLVSPGQVRVV
                     GSARPFPTRSGFGSTLPLLVAEEDEFSQVTLQESDRVQFMVVDCRGIIRFVAPLAPGS
                     EDLEAAVDEVLAAVEQAEHSRTQRR"
     gene            1270..1704
                     /locus_tag="VMZ53_22520"
     CDS             1270..1704
                     /locus_tag="VMZ53_22520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31310.1"
                     /translation="MKRRLPDLSHVLDDSRPMMRTLAVREKRIHTRFDKAFLVVIGSE
                     LYGDSFAIARNISAGGLLVEMTYAPPLGTVVTVHFQYMREAASPEQETPAMDEIVARA
                     EVKHHHYLNFTGGNDAASSRAIGLRFIEFIDPGDRVEADRIH"
     gene            1766..3331
                     /locus_tag="VMZ53_22525"
     CDS             1766..3331
                     /locus_tag="VMZ53_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31311.1"
                     /translation="MRAAIILAIIAAFWTPALAKDPPAPPPPPKPVDAAVQAVLDRVA
                     KGDKAAIDEVNKLAATHIEDLKSFLQRPHTSPVEVRLELLKSIEADVPDEKGRFVAPQ
                     RKSAKEEKKLDELDWLAELQKKDAATPGVGETIADVVVIRALSASKAIEAAPVIFEAA
                     FWPETMIYRDEVGRYLRKMEPYCIPALMKEAQAGNYDRKRYSNYQLERLDRQEPGKAL
                     SAASGDEQLMIAILEAWRATKHREAVHAVWGKVNADSPRVRAKAREAWRAYIEGPPPK
                     PAPMKKLQLPGGKLTKKEKPLYLTYRELADNELRKWSNELLATDYPIVDPTLDDSDEG
                     KVVKKVQKIDLSAVTKELFDFYDKQRAEQDGKQWAEAEAKAKAGDLAGATTMLDRLLA
                     ANPDHTAKAQMALVYFDQAKQLEKASKWAEAAAAYSKANGLAPQGPNANDALAAHHYM
                     LGKALEAQGKDGGPDYRKAIALKPDYAPAKEAAVRASGGHKPTWMIYGAILAILLAGG
                     FFAAAMVRRRAAH"
     gene            3350..3961
                     /gene="tmk"
                     /locus_tag="VMZ53_22530"
     CDS             3350..3961
                     /gene="tmk"
                     /locus_tag="VMZ53_22530"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HUS31312.1"
                     /translation="MTPGRLIVIEGLDGAGTTTQTKRLVEHLNASGRRAHATREPSDG
                     PVGRLIREMLTGGHAIEGERLSQGTFGLLFAADRLDHMQREVEPQIKAGALVISDRWY
                     HSSLAYQGTGADRDWITTINARARKPELTIFLQVRPEVAAQRRAAARRKEELFEDVRM
                     QQEVDAGYKATIAELKALGERIEILDGEQSEDQVFAAIVSLVQ"
     gene            4026..4556
                     /locus_tag="VMZ53_22535"
     CDS             4026..4556
                     /locus_tag="VMZ53_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31313.1"
                     /translation="MRALLASVVFVIACGPKVAPKSTVFDEDLPTEKVVANAEPAAVE
                     PPRPEAPPGKGLRNGTIERAQLIAVLDKGPGAFLRQLEVTAAMDGSRFVGWQLVQLLD
                     PQSPLRDVDVVPQDVLLAVNGKPITRPDQLQMLWDSLRTASEVRALVWRGDAKLELAF
                     AIVEAAPPVAPVQLSK"
     gene            4556..5608
                     /locus_tag="VMZ53_22540"
     CDS             4556..5608
                     /locus_tag="VMZ53_22540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31314.1"
                     /translation="MRCLLLLTLAACGRIDFDPRHDAGVDATSDAVAPRCTEGWLDVP
                     GPRVYGYRVTRIRDGFAIVATDAGSTFVLFVVHDDLTVEPARALQISQIGSLYGVAST
                     DDVVRVAWTEVVPTTYHVGEFALDGTMLSARLTPQLGVTSSSGLDLAVATGTMAGTID
                     VWGIDATGTVTPVHVLTTASTSPSAVARTSNGFTGAWTAGTAHYLTAIDDGGTITVAD
                     RMIGDGSSMSLAWTGQRHVVVRQDVPSALIAAVYDANLDLVGGPTTLAITGWSGWTMA
                     WNGTDIGGVGFANIVGVRDLRFGIIQSDSTLGETVGFGDGMANVLTGPSIAAGPGRFA
                     MVWNADQQLRFAVACR"
     gene            complement(5605..6390)
                     /locus_tag="VMZ53_22545"
     CDS             complement(5605..6390)
                     /locus_tag="VMZ53_22545"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HUS31315.1"
                     /translation="MPKLAVARLVPHFGRLGRAERWKRATRARPRIWAHRGASAHAPE
                     NTLAAFELAKSAGADGIELDVMLDRDANVVVFHDHELTRLCGRPGRMEELGASVRKEL
                     RVQGEPVPLLADVFDMLGDLEINVEIKVERVARAAQLVKETANVIRRSGRAEQVLVSS
                     FDPIALVQFHRYMPDIALAYLFAQEQGLPIRRGWVGQWIGASVVHPDHRLCTKQMVKA
                     WHTAGMPINAWTVDDEAELRRLDDLGVDGVFTNDPARALAAFG"
     gene            complement(6407..8227)
                     /locus_tag="VMZ53_22550"
     CDS             complement(6407..8227)
                     /locus_tag="VMZ53_22550"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS31316.1"
                     /translation="MSHAFVTGATGLIGRWLVPELVRRGSQVTVLLRNARERSAEYLQ
                     WVASHGADATAVRVVDGDLASPGLGLSEADRVHLATLTDVFHAGALMKWRMARDVAKA
                     ANVDGTQAVVDLALASRALRRFVHVGGFKAATAEAWTSRGIAHDAPFDHALYEGLYNR
                     LGGYEASKLAADHLVRDAARTRGLPLTQVHPGGVIGDSCTGETTQFFGFAPLVQDLWY
                     GKLPAVPGGRRTWLPLTTVDFLASFMARLPELADTAGGSYTVLDERTPLLVDLVALIG
                     ERIGVAAPRRRVPISIVKRVMRSRREELSFLSDERHDTQATVAIAARMNLAWPSITDA
                     LHRNIDFIIASRFGAQPVHADAGLDRIAGSQTFVTGTTTSPDRVLLHGLPLDSDSWDA
                     LDQQLGGSSLRADLPGLGRSAPAAATPAEWTSSLLRSVARPTMVVAHSLGTLYAIEYA
                     VAHPERVEALVLVSPFFLQAPPPSMMRWAPAARSVGRLLRRKHIEQLVAGDAHATTPV
                     LDGPATHLARPGAGARFGEALAFAHARRKSLQQALAELATRIPIVIIHGERDALVDAA
                     PSGVRVVTLPGTGHFPQLDQPAAVAAVIRELASATHARAA"
     gene            complement(8224..8817)
                     /locus_tag="VMZ53_22555"
     CDS             complement(8224..8817)
                     /locus_tag="VMZ53_22555"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS31317.1"
                     /translation="MGRTQRRGETARTRLLATTAELLHKQGYSSTGMNQILDESKAPK
                     GSLYFYFPGGKEELVAAALSQASEGLSATLQQLLDASPTPVAAVKAVIAFFKGQLEHS
                     HFTKGCPVATVALEQSATSDALHEVCAGAYRGWHRLVEKRLVRDGYSPARADSLANFF
                     LTTLEGALLVSRAHRSTAPLVAAGNELIRILEQGDHA"
     gene            8894..10654
                     /locus_tag="VMZ53_22560"
     CDS             8894..10654
                     /locus_tag="VMZ53_22560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31318.1"
                     /translation="MPDEETDATVPDVPARRDSERLEPQRRSARKTGDRFVLGEALGR
                     GGMGEVLDARDEQIGRTVAIKRMLADAPTDAQMQRFLREAKIQGQLEHPAIVPVHELG
                     RDTDGRPYFAMKKLAGTTLADIVDLDDGTKYPRNRLLRAFSDVCLAIELAHTRGIVHR
                     DLKPSNIMLGDFGETYVLDWGVAKVLGESSDVALDVTDSANATRAGAAIGTPGYMAPE
                     QARGEKTIGPRADIYALGCVLFEILAGDRLHPRGEAGLVMTLEQTDARPSSRAPDREI
                     PPELDALCVEATQLDPANRISSARELGERVQRFLDGDRDLELRRTIARQHLDAANAAL
                     AAGDRKSAMREAGRALAFDSTLSEAAQVITTMLVEPPREPPPEVVSEMAKEDEGLVRS
                     SARVGAIANSIYVLLVPMLLIAGSVPLLYSLAISALAVLGTGYLMYMARPGGAYRLWP
                     IALVDTLLLVVVARLLSPFVFGIAASAVTVVGLMAGPAVSLRRGIAVATMLSLAVLLP
                     WLGEQVGWLSSTIDVARGEMTLRSPLYWSPAMTHLALVLATIVGTFAALALTRQLRFA
                     ERDARLRMHMQAWQLRQLVS"
     gene            complement(10655..11833)
                     /locus_tag="VMZ53_22565"
     CDS             complement(10655..11833)
                     /locus_tag="VMZ53_22565"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HUS31319.1"
                     /translation="MTPTGIVVQKYGGSSVADVDKIRLVAQRIARTKAAGKKVVVVVS
                     AMGKTTNSLIEKAKAISPSPSRRELDMLLTCGEREAMSLLSMACAEEGLESISFTGSQ
                     AGILTNDRHSGARIVEVRPFRIEDELDRGKVVIVAGFQGVSYKREITTLGRGGSDTTA
                     VALAGALRADYCEICSDVDGVYSADPRVVDAAQLLESISHDEMLELAAQGAKVLHDAS
                     VEFARKSGIALYARATNKDGGGTRIDFTPERERVVAAVTGQSSLIRVRVSGGAAVEHC
                     LQIIEAASIPLTHLDLEGKELRCWFTIADVPDWGEVRKRLDTVLAGQIEFGEEGAVTM
                     VGHGIGGNPQIINKARASAVGAGVPLNAVSVSPLRVTLWCDRQHVDNLVRSLHKTFVE
                     "
     gene            11884..13986
                     /locus_tag="VMZ53_22570"
     CDS             11884..13986
                     /locus_tag="VMZ53_22570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31320.1"
                     /translation="MTLRAAVLLLAISMMACGSSAKRTPAWPDAPMELRDDSDREQAI
                     DQLWVLPNGAVRDAVRAKIADAIIDRINDALEDDKPYNAEQLLFQLCTLWQTDPGKVG
                     AGLARHAKVIHTLRATFAKSGALEPTIATLTLLAELDSAHRDEHLAELDEVLHFSDDL
                     EAAENGPEAQRAQPLELLQPTVLALPLGWIVDKYVALMTERQRVISELINKNGASIQL
                     VRAHHDMLATSLRIAIALARAGRTEQINDKIVDIKGLGQSKELQIRAELVAERPTAEA
                     YGDLAEQMRDDKDNADAAAALAVCLAGLKKFPTDANLLSAAAADAATLGRVDQPIELY
                     EAAIKAQGGDVDSALALRLGKLYAERIARLAFGGRPAAAKKEWKELEQYTRRQKSPPQ
                     VWSVVAAGGETALGRGLLSQGKLREAERELVASLDRAPSIDAYETLATIHFKTDRLSS
                     ATRYATEGIKLLGVTKGDQVRRAKLDRIAADVARTAGRSRDAATLYLDALRTWGALGP
                     DSELPPAVRGERKLEFARSLWFVGESGKAVNLVFDAIDADPSNAANYASGVAFLISVG
                     AYADALDIVHRALTQPDVSEFYRVYMCLWALAEATQRGEPRDRQSVEFLATRQGDLWY
                     ELLAKAATGRADVGQLTAAATTGPRQAELAFYRVTLGLDAEAKSPAGRKKLLEEVVMA
                     RLVMDAEYDLARRYLSRP"
     gene            13992..14906
                     /locus_tag="VMZ53_22575"
     CDS             13992..14906
                     /locus_tag="VMZ53_22575"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUS31321.1"
                     /translation="MIDDEAGEFLRARLTPRALAAWAGTDRLSALPLSVPQERALTKA
                     GALLELFVGGGLVRRDPSWPDFGELVVERDGDLRATVSVLPLGKGLIVCDRLDTSDGL
                     DYVCWPDDSSYHLAHAIPPGRRARWLDLGCGSACAEVMRPELATSIVAADLNARATRY
                     AAMGLELSGISHAEVVTADLATGLDGPFELVTCNAPIPGNSASEGIDSRWRSTDGGFF
                     ARLFDDAKRVVSGDGMVVVHGATEALQAEVMGLPGERVVVSYTPEDLFGFGVLWWRPG
                     AADRLVWKRRELTADRPHITFDDRLAVL"
     gene            14940..16175
                     /locus_tag="VMZ53_22580"
     CDS             14940..16175
                     /locus_tag="VMZ53_22580"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUS31322.1"
                     /translation="MAQKTLSIVVLILAFAVGCNQSRTIETPAPPIDKTRLSHSQHAM
                     IRDCTSCHRGGERPGAQDHAPCDDCHKQAFLEKPGELCKTCHTKVTDTPVTAPLRPYP
                     VEDLWQSEPPRFSHKLHMDLGRMEAGVGFHVTCIDCHQRDGKLARPDHATCSRCHAPE
                     AKLGNAPAMGDCGGCHKKGLHERFRKRLIKGDLQFDHEHHRADRRGTAVKCEECHAES
                     AASASYTDHAPPRVANCVGCHDDSQRVPAELRMRMCQTCHSQITQTVISIAPRSHLPA
                     TERPIDHTLAFRRDHAEVAEREGARCAACHTQMSGNPRDLCDECHQTMRPFDHTITFR
                     EMDHGPEAAATRDRCARCHVVEFCTACHSQRPRSHGFPGTFLEHHGGSARVNIRSCLT
                     CHDETTDCAQCHAVRGVMP"
     gene            16172..17971
                     /locus_tag="VMZ53_22585"
     CDS             16172..17971
                     /locus_tag="VMZ53_22585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31323.1"
                     /translation="MSRLIIVTLVALAAGNAFADAPNLKLTREPPPRHLRMRPRTAAT
                     TTPGPAPVATDVPESGAQVNATGEATPLGSLRDLRRPISIRFSLGYVVDGTGLRNNSA
                     DPACDLTTSCTLNINQQPVRSSEISRLRAYALGEGDFSSRGVVFPSLSTYLATKFQIA
                     KRTTGFAFADADQLDRKPIGPPVATWFERSGIEPRQFWAEVKDFLPDARLAPLRLRAG
                     EIYVYGPWVLHMYGSIAAWEGKLVKASLYGGSRVPDFTNSGFFDRKNRSGIGGGSLNF
                     DLRALKTPIPFAVGVEALKFTAIGGYDASAANHAALQLDWRPRKDIALISRFRTLDGE
                     LANEYVQLRTRYKLVSNLVFDYSHRHSRDWRWDPSITSNDSMTARRYLDLGPNIPQSI
                     ISGRAGTLIKENVDVLVRGALAWDGANSDIERNTFMARYFELGTALEVRLRRTVGVGL
                     SALTRQTERTATVTGEIVDIPNQIDPIPIRYSPEMGEIGFTELGTTLRLSLGARRLSA
                     LVEAYGRRTRYALDYCAPAIQMDGTYDPRCMSALDTGIQSRDIRGGGRFTLDAWVGSQ
                     LRLFASYEISSRIDFQRELSGFKSLRLVMEGNY"
     gene            17971..19461
                     /locus_tag="VMZ53_22590"
     CDS             17971..19461
                     /locus_tag="VMZ53_22590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUS31324.1"
                     /translation="MRALVVLLLLTGVAAAEAPFGFDHVIHARDVDVKGMDAIACTRC
                     HAEKQGKLVGKPTHTSCFGQCHGPLPKPPARGGKLAEGDRTRICQSCHSDAQLTAKYS
                     GKLPASYPPFTIDPDFGVTFGHKQHAAVTCVSCHDVRDKPAKGAVHARCASCHDGSGA
                     RGRGPAMAKCTTCHPAGVGRPQPPQLAVVKDSVESSFSHAKHATRGAQGKDCGTCHAT
                     IRETDDFQLPRPTVKTCALAGCHDAKAAFATTVACTRCHDRAPTDHFEVSRPTARFEH
                     AGNHEQLVKSTPCATCHPVLPSGEVRVVGHAPCVECHSEDFTARRPQTCGACHNATEP
                     WRKLVADRAPPRQTEFGASLDHTKHKAECATCHTLRTETAELRTSRGHHACTGNGCHA
                     PKGGAAPQITECAACHRIGLSIERLTARISAAWSVRATFQHATHRTTKNGNDLACAAC
                     HEDLAGDLLATPTPKKAACIACHDTNKQAFKVTGTTCSRCHGARTR"
     gene            19470..19814
                     /locus_tag="VMZ53_22595"
     CDS             19470..19814
                     /locus_tag="VMZ53_22595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31325.1"
                     /translation="MPRWQRAYILATCALIGGAFAYAACDWGRWPRLTYRPLTGEIMM
                     KTPPGAITVNYLGTVAWGLGGAAAGALVGALLCAIFKRPWPGRALHLFGAWALTGVVL
                     AGSYYTWNLWPW"
     gene            19854..20519
                     /locus_tag="VMZ53_22600"
     CDS             19854..20519
                     /locus_tag="VMZ53_22600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31326.1"
                     /translation="MTTKLAAAVLLALTSTAAAQTYVARDVSYAEPPAPKAPRMVDAR
                     LGLMLGGSDVGDADGFSAGASGALGYRIGDVTLRGMFDYYKVGDGSDEALSRHGRATR
                     VGGAVRYSFANTGYDSSAAVDFWGELGAGYEHVAWRHGGILERPSGELAVGLDLGRRG
                     ERNARGERKEIGYFMAFRSLVAHGPEMEGATAMCGGPCTEATKPPRTDVSMFFELGVH
                     WGR"
     gene            20547..>21304
                     /locus_tag="VMZ53_22605"
     CDS             20547..>21304
                     /locus_tag="VMZ53_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31327.1"
                     /translation="MQIANGVLRHSLVPSCLAFLAACTGGGGMQKSAVYSGGGGTAAA
                     PSSTIAGDSSYSPPAHAETYYEPARPSQQSTIVQNDPAPERPGLGTTFGEEVYAPISF
                     APFQRSSGSPWAEVALHYNDADGVNAHAQYLGVTPQPLEIYAGDGSIAVALVDEMGRT
                     LPGVDAGGRTLIMGEDGAHYRIVVRNATTARFEIVASVDGLDVIDGKPADPNRRGYIV
                     DPHDTLVIDGFRTSDANVAAFRFGRVADSYAAQTS"
BASE COUNT         3643 a         7095 c         7125 g         3441 t
ORIGIN      
        1 cgaacgtccg aacggttgcg gtcatcggcg ttcgtaagcg taggcgtaag cgtccgcgta
       61 agcgagcacc cgtcctgatc tttcgctgct gcgaaaggac cgcacaaacc acgagcagat
      121 acgggaaagg gcgtccctct cggccttgct tccttgcgtt ccatccgagc tctcgttgcg
      181 cagacccgag agagcggaac gccggaaccg cttacgctta cgcttacgct tacgctcgct
      241 tgcgcttgcg ggctagcggc gttgcgtgcg cgaatgctcg gcttgctcga cggcagcgag
      301 cacttcgtcg acggcagctt cgagatcctc ggagcctggg gcgagcggag cgacgaagcg
      361 aatgatgccg cggcaatcga cgaccatgaa ctgcacgcgg tcgctctcct gcagcgtgac
      421 ctgcgagaac tcgtcttctt cggcgacgag gagagggagc gtcgagccga agccggagcg
      481 cgtcgggaac ggtcgcgccg agccaacgac gcggacctgg cctgggctga cgagcgtgct
      541 ctggagcgga tcgctgttgt gtgcggtgat gccaatgacg tcgggcgtct cgtctttgat
      601 gcggtcgcga atgccgtcgg cgaccttgtc gatgaccgag ccgcagctga tgcagcgatc
      661 gaggcggaac cacgcgagca cggttgtctt gccgcgaagc tcgccgagat cgatctcgtg
      721 atcgggattg tcgatgtcct tcgtctcgag cccgggcacc gcttggccga cgaagcagcg
      781 atgctggaca tcggcgcgcg tcgagagcgt gaccttcacc gtcgctggac ggccgttgcg
      841 ccgcagctcg aaggcagcga cgtcacccgg cgcgtgcgcc atgatcttct cgcgcagcac
      901 atcgcacgac gagcgacgct gctgcggcgc ggtgtgcgga tcgacgagcg cgttgccgtc
      961 catgccgacg acgaggtcgc tatcgcgaag gccgccatct tccgccgggc tgcagcgcgt
     1021 gatgcccgtg atctcgcagc caccggcgaa atcgaccatc gcgatgccga ggaaagcggg
     1081 gttgctctgg gtcgatggcg ccgcgctcgc cgcacccgcg gcaagcagca cgacgatgat
     1141 cgagaccagg cttcgcacgc ggtgagactg cctgaaggac ggtcgggccg ccaagaacat
     1201 tcgtgtcgtc tgatgtagga caaggtgaga tttttgactc catctcggcc gggctcgatc
     1261 attatttcgg tgaagcgacg cctccccgat ctgtcccatg ttctcgacga cagcaggccc
     1321 atgatgcgga cgctcgccgt ccgcgagaag cgcatccata cccgcttcga taaggcgttc
     1381 ctcgtcgtga tcggatcgga gctgtacggc gacagctttg cgatcgcacg caacatctcc
     1441 gccggtggcc tgctcgtcga gatgacgtac gcgccgccgc tcgggaccgt cgtgaccgtc
     1501 cacttccagt acatgcgcga ggcagccagc cccgagcagg agaccccggc aatggacgag
     1561 atcgtcgctc gcgccgaggt gaaacatcac cattacctga acttcaccgg gggtaatgac
     1621 gcggccagct cgcgcgcgat cggcctgcgc ttcatcgagt tcatcgatcc gggagatcgc
     1681 gtcgaagccg atcgaattca ctgactaggt cgagcgttcc gtccctaact tgcgccgacg
     1741 gcgacgaacc gctaatccct ggtctgtgcg tgccgcgatc attctcgcga tcattgcggc
     1801 gttttggacg cccgctctcg ctaaagatcc gccggcgcca ccgccgccgc caaagcccgt
     1861 cgatgctgcg gtgcaagccg tgctcgatcg cgtggcgaaa ggtgacaaag ccgcgatcga
     1921 cgaggtcaac aagctcgccg cgacgcacat cgaggatctg aagtcgttcc tccagcgtcc
     1981 gcatacgtcg cccgtcgaag tgcggctcga gctactgaag tcgatcgagg cggacgtgcc
     2041 cgacgagaag ggccggttcg tcgcgccgca gcgcaagagc gccaaggaag agaagaagct
     2101 cgacgagctc gactggctgg ccgagctgca gaagaaagac gcggcgacgc cgggcgtggg
     2161 cgagaccatc gcggacgtcg tcgtgattcg cgcgctgtcg gcgtccaagg cgatcgaggc
     2221 ggcgcctgtg atcttcgagg cggcgttctg gcccgagacg atgatctatc gcgacgaggt
     2281 cggtcgttat ctccggaaga tggagccgta ctgcatcccg gcgctcatga aagaagcgca
     2341 ggcgggcaac tacgatcgca agcgctactc gaactaccag ctcgagcggc tcgatcggca
     2401 ggagccaggc aaggcgctgt ccgcggcgag cggcgacgag cagctgatga tcgcgatcct
     2461 cgaggcgtgg cgcgcgacga agcatcgcga ggccgtgcac gcggtgtggg gcaaagtgaa
     2521 tgcggactcc ccgcgcgtgc gtgcgaaggc gcgtgaagcg tggcgcgcgt atatcgaggg
     2581 cccgccgcca aagccggcac cgatgaagaa gctgcagctg ccgggcggca agctgacgaa
     2641 gaaagagaag ccgctgtacc tcacgtatcg cgagctagcg gacaacgagc ttcgcaagtg
     2701 gagcaacgag ctgcttgcga cggactatcc gatcgtcgac ccgacactcg acgacagcga
     2761 cgagggcaag gtcgtcaaga aggtccagaa gatcgatctg tccgcggtga cgaaggagct
     2821 gttcgacttc tacgacaaac agcgcgccga gcaggacgga aagcagtggg ccgaggccga
     2881 agcgaaggcg aaggccggtg acctcgccgg tgcgacgacg atgctcgatc gcctgctcgc
     2941 cgcaaatccc gatcacactg cgaaggcgca gatggcgctc gtctacttcg atcaggccaa
     3001 gcagctcgag aaagcgagca agtgggccga ggcggccgcg gcgtactcga aggcgaatgg
     3061 cctcgcgccg cagggaccga acgcgaacga cgccctcgcc gctcatcact acatgctcgg
     3121 caaagcactc gaagcgcagg gcaaagacgg cggccccgat taccgcaagg cgatcgcgct
     3181 gaagcccgac tacgcgcctg cgaaggaagc ggcggttcgt gcaagcggtg gtcacaagcc
     3241 gacgtggatg atctacggcg cgatcctcgc gatcctcctc gccggcggtt tcttcgcggc
     3301 ggcgatggtg cgacgtcgag ccgcgcacta ggcgtgatat gactcggcgg tgactccggg
     3361 tcggctcatc gtgatcgaag gtctcgacgg tgcgggcacc acgacgcaga cgaagcgtct
     3421 cgtcgagcac ctcaatgcga gtggccgcag ggcgcacgcg acacgcgagc cgagtgatgg
     3481 tcccgtcggt cgcctcatcc gcgagatgct cacgggtggc cacgccatcg agggtgagcg
     3541 cctctcgcag ggcacgttcg gcctgctgtt cgccgccgac cgcctcgatc acatgcagcg
     3601 cgaggtcgag ccccagatca aagccggcgc gctcgtgatc tccgatcgct ggtaccactc
     3661 gtcgctcgcc tatcagggca ccggcgcgga ccgcgactgg atcacgacga tcaatgcgcg
     3721 cgcgcgaaaa ccggagctga cgatctttct ccaggtgcgg ccggaagttg ctgctcagcg
     3781 tcgagccgcg gcccggcgta aagaggagct gttcgaggac gtgcggatgc agcaggaggt
     3841 cgatgccggc tacaaggcga cgatcgccga gctgaaagcc ctcggtgagc gcatcgagat
     3901 cctcgacggc gagcagtcgg aagaccaggt cttcgccgcg atcgtgagct tggtccaata
     3961 gggaccgcgg cgacaacctg cgttgaaact tgcgccggag cacgtcgccc ggcacgttca
     4021 taggcatgcg cgccctgctc gcctctgtcg ttttcgtgat cgcatgtggc ccgaaggtcg
     4081 cgccgaagag cacggtgttc gacgaggatc tcccgacgga gaaggtagtc gcgaacgcgg
     4141 agcctgcggc tgtcgagcca ccgcgtcccg aggcgcctcc aggcaagggt ctgcgcaacg
     4201 gcacgatcga acgcgcgcag ctgatcgcgg tgctcgacaa gggacccggc gcgttcctgc
     4261 gccagctcga agtaacggcc gcgatggacg gcagtcgctt cgtcggctgg cagctcgtgc
     4321 agctgctcga tccgcagagc ccgcttcgcg atgtcgatgt cgtgccgcaa gacgtcctgc
     4381 tcgccgtcaa cggcaagccg atcacgcggc ccgatcaatt gcagatgctg tgggactcgc
     4441 tacgaacggc gagcgaggtg cgcgcgctcg tgtggcgcgg cgatgcgaag ctcgagctcg
     4501 cgttcgcgat cgtcgaagcg gcgccgccgg tggcgcccgt gcaactctcg aagtagtgcg
     4561 ctgcctgctg ctcctcacgc tcgctgcgtg cggtcgaatc gattttgatc ctcgccacga
     4621 tgccggcgtc gacgccacca gcgatgcggt tgcgccacga tgcaccgagg gctggctcga
     4681 tgttccgggt ccgagggtgt acggctatcg cgtcacgcga atccgtgatg gctttgccat
     4741 cgttgccacg gacgcaggct cgacgttcgt gctcttcgtc gtgcacgacg atctgacggt
     4801 cgagccggcg cgcgcgctgc aaatttcgca aatcggcagt ctctatggtg tcgcctcgac
     4861 ggacgatgtg gtgcgcgtcg cgtggaccga ggtcgtcccg accacgtacc acgtcggcga
     4921 attcgcgctc gatggaacga tgctctccgc tcgtttgacg ccgcagctgg gagtgacatc
     4981 gagctccggt ctcgatctag cggtcgcgac cgggacgatg gccggcacga tcgatgtgtg
     5041 gggcatcgat gccacaggaa cggtcactcc agtgcacgtc ttgacgaccg catcgacctc
     5101 gcccagcgca gtcgcgcgca cgagcaatgg cttcaccgga gcgtggacgg ccggaaccgc
     5161 gcactacctc acggccatcg atgacggagg cacgatcacc gtggccgatc ggatgatcgg
     5221 ggatggttcg tcgatgtcac tcgcgtggac ggggcagcgg cacgtcgtcg tgcggcagga
     5281 cgtgccaagc gcgctcattg ccgccgtcta cgacgccaac ctcgatctcg tcggcggtcc
     5341 gacgacattg gcgatcactg gctggagtgg gtggacgatg gcgtggaatg gcaccgacat
     5401 cggcggcgtc gggtttgcga acatcgtggg cgtgcgggac ctgcggttcg gcatcatcca
     5461 gtcggacagc acgctcggcg aaacggtcgg gtttggcgac ggcatggcga acgtacttac
     5521 aggtccttcg atcgccgctg gtccgggccg cttcgcaatg gtgtggaacg cggaccagca
     5581 gctgcgcttc gccgtcgcgt gtcgctagcc gaacgcggcg agcgcgcgcg caggatcgtt
     5641 cgtgaagacg ccgtcgacac cgagatcgtc gaggcgacga agctcggcct catcatcgac
     5701 ggtccatgcg ttgatcggca ttccggcggt gtgccacgcc ttcaccatct gcttcgtgca
     5761 cagccgatgg tcgggatgga cgacgctcgc gccgatccac tgaccgaccc agccgcgacg
     5821 aatcggcagg ccttgctcct gcgcgaagag gtatgcgagc gcgatgtcgg gcatgtagcg
     5881 atggaactgg acgagcgcga tcggatcgaa gctcgagaca agcacttgct cggcgcggcc
     5941 gctgcggcgg atcacgttcg ccgtctcctt gacgagctgc gcggcacgcg cgacgcgctc
     6001 caccttgatc tcgacgttga tctcgaggtc gccgagcatg tcgaacacgt ccgcgagcag
     6061 cggcaccggc tcgccttgca cgcgcagctc tttgcgcacg cttgctccga gctcctccat
     6121 gcggccgggg cgaccgcaga ggcgggtcag ctcgtgatcg tggaagacga cgacgttcgc
     6181 gtcgcgatcg agcatcacgt cgagctcgat gccatcggcg ccggcagact tcgcgagctc
     6241 gaaggcggcg agcgtgtttt ccggcgcgtg tgccgatgca ccgcgatggg cccagatgcg
     6301 ggggcgcgca cgcgtggcgc gtttccaacg ctcggcgcgt cccagccggc caaagtgcgg
     6361 cacgagccgc gcgacagcta gctttggcat gtgccgctag cttagctcac gcggcgcgtg
     6421 cgtgtgtcgc ggacgcgagc tcgcgaatga cagcggcgac ggcggcaggc tgatcgagct
     6481 gcgggaagtg gcccgtgccc ggcagcgtga cgacgcggac gccggacggt gcggcatcga
     6541 cgagcgcatc gcgttcgcca tggatgatca cgatcggaat gcgcgtcgcg agctcggcga
     6601 gcgcttgctg caagctcttg cgacgagcat gcgcgaaagc aagcgcttcc ccgaatcgag
     6661 cgcctgcgcc cgggcgcgcg aggtgcgttg ctggaccgtc gaggacgggc gtcgttgcgt
     6721 gcgcgtcgcc ggcgacgagc tgctcgatgt gcttgcgtcg cagcaggcga ccgacgctgc
     6781 gagctgccgg cgcccaccgc atcatcgacg gcggcggcgc ctgcagaaag aacggcgaca
     6841 cgagcacgag cgcttcgacg cgttcgggat gcgcgaccgc gtattcgatg gcgtacagcg
     6901 tgccgagcga atgcgcgaca accatcgtcg ggcgcgcgac ggatcggagc agcgagctcg
     6961 tccactcggc aggcgtcgcc gcggcaggcg cggagcggcc gaggcccggt agatccgcac
     7021 gcagcgacga gccaccgagc tgctgatcga gcgcgtccca cgagtcgctg tcgagcggta
     7081 gtccatgcag cagcacgcga tcaggcgagg ttgtggtccc ggtgacgaac gtttgcgagc
     7141 ccgcgatgcg atcgaggccg gcatccgcgt gcaccggctg tgcgccgaag cgcgacgcga
     7201 tgatgaagtc gatgttgcgg tgaagcgcgt cggtgatgct cggccacgcc aggttcatgc
     7261 gcgcagcgat cgcaacggtc gcctgcgtat cgtggcgctc gtcgctcaag aacgagagct
     7321 cctctcggcg cgagcgcatc acgcgcttca cgatgctgat cgggacacgg cgacgcggcg
     7381 cagccacacc gatccgctcg ccgatgagtg cgacgaggtc gacgagcagc ggcgtacgct
     7441 cgtcgagaac cgtgtagctc ccgcccgcgg tatcggcgag ctcgggtagc cgcgccatga
     7501 agctcgcgag aaaatcaacg gtcgtgagcg ggagccacgt gcgacgtccg ccaggaacgg
     7561 cgggcagctt gccgtaccag aggtcttgca cgagcggcgc gaacccgaag aactgcgtcg
     7621 tctcgccggt gcacgaatcg ccgatcacgc cgcccgggtg cacttgcgtc agtggcaggc
     7681 cgcgcgtgcg agcagcatcg cggacgaggt gatcggcggc gagcttcgac gcctcgtaac
     7741 caccgaggcg gttatagagc ccttcataga gcgcatgatc gaacggcgca tcgtgagcga
     7801 tgccgcggga cgtccatgcc tcggcggtcg cagccttgaa gccgccgacg tgaacgaaac
     7861 gccgcaacgc gcgtgaagcg agcgcgaggt cgaccacggc ctgcgtgccg tcgacgtttg
     7921 cagccttggc gacatcccgc gccatccgcc acttcatgag agcaccggcg tggaacacgt
     7981 cggtgagcgt cgcgaggtgc acgcgatcgg cttcagagag gccgaggccc ggcgacgcga
     8041 gatcgccatc gaccacgcga acggctgtcg catccgcacc gtgactcgcg acccactgca
     8101 gatattcggc gctgcgctcg cgcgcgttgc gcaagaggac cgtgacctgc gagccgcgac
     8161 gaacgagctc cggtaccaac cagcgaccga tcagacccgt ggcacccgtg acaaatgcgt
     8221 ggctcatgcg tggtctcctt gttcgagaat tcggatgagc tcgttaccgg cggcaacgag
     8281 cggtgcagtc gagcgatgag cgcgactgac gagcagcgcg ccttcgagcg tcgtgagaaa
     8341 gaagttcgcc aggctgtcgg cgcgcgcggg cgagtagccg tcccggacga gacgcttctc
     8401 gacgaggcga tgccacccgc gatacgcgcc ggcgcaaacc tcgtgaagcg cgtcggacgt
     8461 cgcagactgc tcgagcgcga ccgtggcaac cgggcagccc ttcgtgaagt gcgagtgctc
     8521 gagctggcct ttgaagaacg cgatgaccgc cttgacggcg gcgaccggtg tcggcgacgc
     8581 atcgagcaat tgctgcagcg tcgccgacag gccctcgctc gcttgcgaca acgccgccgc
     8641 gaccagctcc tctttgccgc ccgggaagta gaagtagaga gaccccttcg gcgctttact
     8701 ctcgtcgagg atctggttca tccccgtcga cgagtagccc tgcttgtgga gcagctcggc
     8761 ggtcgtcgcg aggaggcgcg tccttgctgt ttctcctcgc cgttgggttc gtcccatgtg
     8821 ttatatatag accagtcgtt atagtttgtc aatggcccgc caacctggcg ggtttacgag
     8881 aggatggcca gcgatgcccg acgaggagac cgacgcgacc gttcccgacg tccccgcgcg
     8941 acgcgattcc gaacggctcg agccgcagcg ccgaagcgct cgcaagaccg gcgatcggtt
     9001 cgtgctcggc gaagcgctcg gacgcggagg catgggcgag gtgctcgacg cgcgcgatga
     9061 gcaaatcggc cgaaccgtcg cgatcaagcg catgcttgcc gacgcgccga cggacgcgca
     9121 gatgcagcgc ttcctgcgcg aggcgaagat ccagggccag ctcgagcatc ctgcaatcgt
     9181 tccggtgcac gagcttggcc gcgacacgga tgggcgcccg tacttcgcga tgaagaagct
     9241 ggccggcacc acgctcgcgg acatcgtcga cctcgacgac ggaacgaagt acccgcgcaa
     9301 tcgcctgctg cgcgcgttct cggatgtctg cctcgcgatc gagctcgcgc atacccgcgg
     9361 catcgtgcat cgcgatctga agccgtcgaa catcatgctc ggcgactttg gcgagacgta
     9421 cgtgctcgac tggggcgtcg cgaaggtgct cggagaatcg tcggatgtgg cgctcgacgt
     9481 gaccgactcg gcgaacgcga cgcgagcggg cgctgcgatc ggaacgcccg gctacatggc
     9541 accggaacaa gcgcgcggcg agaagaccat cggtccacgc gcggacatct acgcgctcgg
     9601 ctgcgtgctg ttcgaaatcc tcgccggtga ccggctccat ccacgcggcg aggccgggct
     9661 cgtgatgacg ctcgaacaaa ccgatgcccg gccgagctcc cgcgcgcccg atcgcgagat
     9721 tccgccggag ctcgatgcgc tgtgcgtcga ggctacccaa ctcgaccccg ccaatcgcat
     9781 ctcgtcggct cgcgagctcg gcgagcgcgt acaacgcttt ctcgacggcg atcgcgacct
     9841 cgagctacgt cgtacgattg ctcgccagca tctagatgcc gcgaacgccg cgcttgcagc
     9901 aggtgatcgc aagagcgcga tgcgcgaagc cggacgcgcg ctcgcgtttg actcgacgtt
     9961 gtcagaggcc gcgcaggtga tcacgacgat gctcgtcgag ccgccgcgcg aaccgcctcc
    10021 cgaggtggtc agcgagatgg cgaaggagga cgaggggctc gtgcgcagca gtgcgcgcgt
    10081 cggagcgatc gcgaactcga tctatgtact gctcgttccg atgcttctca ttgcggggtc
    10141 ggttcccctg ctctactcgc tcgcgatctc cgcgctcgcg gtgctcggca ccggctacct
    10201 gatgtacatg gcgcgccccg gcggcgcgta tcgcttgtgg ccgatcgcgc tcgtcgacac
    10261 gttgctgctc gtcgtcgtcg cccggctgtt gtcgccattc gtcttcggca tcgctgcgtc
    10321 ggcggtgacc gtggtgggcc tcatggccgg tccggccgtt tcgctgagaa gaggcatcgc
    10381 tgttgcgacg atgctctcgc ttgccgtgct cctgccatgg ctcggcgagc aagtcggctg
    10441 gctgtcgtcg acgatcgacg tcgcccgcgg cgagatgacc ttgcggtcgc cgctgtactg
    10501 gagccccgcc atgacgcacc tcgcgctcgt gctcgcgacg atcgtcggga cattcgccgc
    10561 gctcgcgttg acacgccagc tgcggttcgc ggagcgcgac gcgcgccttc gcatgcacat
    10621 gcaagcgtgg cagctgcgtc agctcgtgag ctgattactc gacgaacgtc ttgtggagcg
    10681 agcgcacgag attgtcgacg tgctggcgat cgcaccacag cgtcacgcgc agcggcgaga
    10741 ccgagacggc gttgagcgga acacccgcac cgaccgcgct cgcacgcgcc ttgttgatga
    10801 tctgcgggtt gccaccgatg ccgtggccga ccatcgtgac cgcaccttct tcgccgaact
    10861 cgatctgacc ggcgagcacg gtgtcgaggc gcttgcgaac ctcgccccag tccggcacgt
    10921 cggcgatcgt gaaccagcag cgcagctctt tgccctcgag gtcgaggtgc gtgagcggga
    10981 tggatgccgc ctcgatgatc tgcaggcagt gctcgacggc tgcgccgccc gacacgcgca
    11041 cgcgaatcag tgacgattga ccggtgaccg cggcaacgac gcgctcgcgc tccggcgtga
    11101 agtcgatgcg cgtgccgccg ccatccttgt tcgtggcgcg ggcatacagc gcgatgccgc
    11161 tcttacgggc gaactcgaca gaagcgtcgt ggagcacttt cgcgccttgc gcggcgagct
    11221 cgagcatctc gtcgtggctg atcgattcga gcagctgcgc tgcatcgacg acgcgtgggt
    11281 cggcgctgta gacgccgtcg acgtcgctgc agatctcgca gtagtcggcg cggagtgcac
    11341 cagcaagcgc gacggcggtg gtgtccgagc cgccgcggcc gagcgtcgtg atctcgcgct
    11401 tgtacgaaac gccttggaag ccagcgacga tgacgacctt gccgcggtcg agctcgtctt
    11461 cgatgcggaa cggcctgacc tcgacgatgc gcgcacccga gtggcggtcg ttcgtgagga
    11521 tgccggcctg cgaaccggta aacgagatcg actcgaggcc ttcctcggcg cacgccatcg
    11581 acagcagcga catcgcctcg cgctcgccgc acgtcagcag catgtcgagc tcgcggcgcg
    11641 acggagacgg gctgatcgct ttggcttttt cgatcagcga gttcgtggtc ttgcccatcg
    11701 cagacaccac gacgaccacc ttcttgccgg cggccttcgt gcgcgcgatg cgttgcgcca
    11761 cgagacggat cttgtcgacg tcggcgaccg aggagccgcc gtacttctga accacgattc
    11821 ccgttggagt cacgtcatgc accatacacg atcataacga tctgacgatg ctaccttgtt
    11881 ctcatgacgc ttcgcgccgc ggtgctgctg ctcgccatct ccatgatggc gtgcggatct
    11941 tcggcgaagc gcacccctgc gtggcccgac gcgccgatgg agctgcgcga cgattcggat
    12001 cgcgagcaag cgatcgacca gctgtgggta ctgcccaacg gtgcggtacg cgacgcagtg
    12061 cgcgcgaaga ttgccgacgc gatcatcgat cgaatcaacg acgcgctcga ggatgacaag
    12121 ccgtacaacg ccgagcagtt gttattccag ctgtgcacgc tctggcagac ggacccgggc
    12181 aaggtaggcg ccgggctcgc acggcacgcg aaggtcatcc atacgctgag agcgacgttc
    12241 gcgaagtccg gcgcgctcga gccgacgatc gccacgctca cgttgctcgc cgagctcgac
    12301 agcgcgcacc gcgacgagca tctcgccgag ctcgacgagg ttctgcattt ctctgacgac
    12361 ctcgaagcgg ccgagaacgg ccccgaagcg cagcgcgcgc agccgctcga gctgctgcag
    12421 ccgacggtgc tcgccctgcc cctcggctgg atcgtcgaca aatacgtcgc gctcatgacc
    12481 gaacggcagc gcgtcatctc ggagctcatc aacaaaaacg gcgcgtcgat ccagctcgtg
    12541 cgcgcccacc acgacatgct cgcaacgtcg ctccgtatcg cgatcgcgct cgcccgcgcc
    12601 ggccgcaccg agcagatcaa cgacaagatt gtcgacatca agggcctcgg tcagagcaag
    12661 gagctgcaga tccgcgccga gctcgtcgcc gagcgcccga ccgccgaagc gtacggcgac
    12721 ctcgccgagc agatgcgcga cgacaaggac aatgccgacg cggctgccgc cctcgccgtc
    12781 tgcctcgcag gcctgaagaa gttccccacg gacgctaact tgttgtccgc cgcggccgcc
    12841 gacgccgcca cgctcggccg cgtcgatcaa ccgatcgagc tctacgaggc cgcgatcaaa
    12901 gcgcaaggag gcgacgtcga ctccgcgctc gcgctgcgcc tcggcaagct ctacgcagag
    12961 cgcatcgcgc gcctcgcctt cggtggccgc ccggccgccg cgaagaaaga atggaaggag
    13021 ctcgagcagt acacgcggcg ccagaagtcg ccgccgcaag tgtggtcggt cgtcgccgcc
    13081 ggcggcgaaa ctgcgctcgg ccgtggcctc ctctcgcaag gcaagctgcg cgaagccgaa
    13141 cgcgagctcg tcgcgtccct cgaccgtgct ccttcgatcg acgcgtacga aacgctcgcg
    13201 acgatccatt tcaagaccga ccgcttgtcg tccgcgacgc gctacgcgac cgaaggcatc
    13261 aagctcctcg gcgtaacgaa gggcgaccaa gtgcgccgcg cgaagctcga tcgcatcgcc
    13321 gccgatgtcg cgcgcacggc cggtcgctcg cgcgatgccg ccacgctcta cctcgacgcg
    13381 ctacgcacgt ggggcgcgct cggtcccgac agcgagctgc cgccggccgt ccgcggtgaa
    13441 cgcaagctgg agttcgcgcg ctcgctctgg ttcgtcggcg aatcgggcaa ggcggtcaac
    13501 ctcgtgttcg acgcgatcga tgccgacccg tcgaacgccg ccaactacgc gagcggtgtc
    13561 gcgttcctca tctcggtcgg cgcatatgcc gacgcactcg acatcgtgca tcgcgcgctc
    13621 acgcagcccg atgtcagcga gttctatcgc gtgtacatgt gcctctgggc gctggccgaa
    13681 gcaacccaac gaggcgagcc gcgcgaccgc caatccgtcg agtttctcgc cacacgccaa
    13741 ggcgacctct ggtacgagct gcttgcaaaa gccgcgaccg gccgcgcgga cgtcgggcag
    13801 ctcaccgccg cagcgacgac aggcccgcgc caagcggagc tcgccttcta tcgcgtgaca
    13861 ctcggcctcg atgccgaggc aaagtcacca gccggccgca agaagctgct cgaagaggtg
    13921 gtgatggcgc gtctcgtcat ggatgccgaa tacgacctcg cacgccggta cctctcgcgg
    13981 ccgtgacgca gatgatcgac gacgaggcgg gcgaattcct gcgggcaagg ctcacgcctc
    14041 gcgcactcgc cgcgtgggcg ggcacggatc gcctgagcgc gttaccactc tccgttccac
    14101 aggagcgcgc gctcacgaag gccggcgcgt tgctcgagct gtttgtcggc ggaggactcg
    14161 tacggcgcga tccatcgtgg ccggattttg gcgagctcgt cgtcgagcgc gacggcgatc
    14221 tgcgtgcgac cgtgtccgtg ctgccgcttg gcaagggcct catcgtctgc gaccgcctcg
    14281 atacgagcga tggcctcgac tacgtctgct ggcccgatga ttcgagctac cacctcgcgc
    14341 acgccattcc tccgggacgc cgcgcgcgct ggctcgatct cggatgcggc tctgcgtgcg
    14401 ccgaagtgat gcggcccgag ctagcgacga gcattgtcgc ggcggacctc aatgcgcgcg
    14461 cgacgcgata tgcggcgatg ggcctcgagc tctctggcat ctcgcatgcg gaggtcgtca
    14521 cggccgatct ggcgaccggg ctcgacggcc cgttcgagct cgtgacgtgc aacgcgccga
    14581 ttccgggtaa cagcgcgtcg gagggcatcg actcgcggtg gcgctcgacg gacggcggct
    14641 tcttcgcgcg gctgttcgat gacgcgaagc gggtggtcag cggcgatggc atggtcgtcg
    14701 tgcacggcgc gaccgaggcg ctgcaggccg aggtgatggg cttgcccggc gagcgcgtcg
    14761 tcgtgagcta cacgccggag gacctgttcg gctttggcgt gctctggtgg cgccccggcg
    14821 cggcggaccg gctcgtatgg aagcggcgcg agctgacggc cgatcgcccg cacatcacgt
    14881 tcgacgaccg cctcgccgtc ctctgatcgg cgctgtgata ccgtcgagcc gcctcgggga
    14941 tggctcagaa gacgctctcc atcgtcgtcc tgatcctggc gttcgcggtg ggctgtaacc
    15001 agtcgcgcac gatcgagacc cctgcgcctc cgatcgacaa gacgcggctg tcgcatagcc
    15061 agcacgcgat gattcgcgac tgcacgagct gtcaccgcgg cggcgagcga cccggcgcgc
    15121 aggaccatgc gccgtgcgac gactgccaca aacaggcgtt tctcgaaaaa cccggcgagc
    15181 tctgcaagac ctgccacacg aaggtgaccg acacgcccgt gacggcgccg cttcggccgt
    15241 atcccgtcga ggacctgtgg cagtcggagc caccgcggtt ctcgcacaag ctgcacatgg
    15301 acctcggtcg catggaggcc ggcgtcggct tccacgtgac gtgcatcgat tgtcatcagc
    15361 gcgacggcaa gctcgcgcgg cctgaccatg caacgtgctc gcggtgtcat gcccccgagg
    15421 ccaagctcgg caatgcaccc gcgatgggcg actgcggggg ttgccacaaa aagggcctgc
    15481 acgagcggtt tcgcaagcgg ctcatcaaag gcgacctgca gttcgatcac gagcaccatc
    15541 gggccgatcg ccgcggcacg gccgtgaagt gcgaggagtg ccacgccgag agcgcggcat
    15601 cggcgtcgta tacagatcat gcgccaccgc gcgttgcgaa ctgcgtcggg tgccatgacg
    15661 acagccagcg ggtgcccgcc gagctgcgga tgcgcatgtg ccagacgtgc catagccaga
    15721 tcacgcagac ggtgatctcg atcgccccgc gcagtcactt gcctgcgacc gagcgaccca
    15781 tcgatcacac gttggcgttc cggcgcgatc atgcagaagt cgccgagcgc gagggtgcac
    15841 ggtgcgccgc gtgccacacg cagatgtccg gcaacccgcg cgacctctgc gacgagtgcc
    15901 atcagacgat gcgcccgttc gatcacacga tcacgttccg cgagatggac cacggccccg
    15961 aggctgcggc gacgcgcgat cgctgcgcgc gctgtcacgt cgtcgagttc tgcaccgcgt
    16021 gtcactcgca gcggccgcgc tcgcacggat tcccaggcac gttcctcgag catcatggcg
    16081 gctcggcgcg tgtgaacatt cggtcgtgcc tcacgtgtca tgacgagacg acggactgcg
    16141 cacagtgcca cgcggtgcgc ggagtcatgc cgtgagccgc ctgatcattg tcacgcttgt
    16201 cgcgcttgct gcgggcaatg cgtttgccga cgcgccgaat ctgaagctca cgcgcgagcc
    16261 acctccccgc catctgcgca tgcggccgcg cactgcggcg acgacgacac ctggtccagc
    16321 gcccgtcgcg acggacgtgc cagagagcgg tgcgcaggtg aacgcgaccg gcgaagcgac
    16381 tccgctcggc agcctgcgcg atctgcgacg gccgatctcc attcgcttca gcctcggtta
    16441 cgtcgtcgac ggcaccggcc tgcggaacaa ctccgccgat cccgcttgcg acctgacgac
    16501 atcgtgcacg ctcaacatca accaacagcc ggtgcgctcg tcggaaatct cgcgactccg
    16561 cgcatacgca ctcggcgaag gcgacttcag ctcgcgcggc gtcgtgttcc cgagcttgtc
    16621 gacgtacctc gcgacgaagt tccagatcgc gaagcggacg acgggcttcg cgttcgccga
    16681 tgcagatcag ctcgaccgca agccgattgg cccgccggtc gcgacgtggt tcgagcgcag
    16741 cggcatcgag ccgcgccaat tctgggcgga agtgaaagac ttcctacccg acgctcgcct
    16801 cgcgccgctg cgtctgcgtg ccggcgagat ctatgtctac ggaccgtggg tcttgcacat
    16861 gtacggctcg atcgcggcgt gggaaggcaa gctcgtcaag gcaagcctct acggcggcag
    16921 ccgtgtgccg gacttcacga acagcggctt cttcgacagg aagaatcgtt cgggcatcgg
    16981 cggcggctcc ctcaacttcg atctgcgcgc gctgaagacg ccgattccgt tcgccgtcgg
    17041 tgtcgaagcg ctgaagttca ccgcgatcgg tggctacgat gcgagtgccg ccaatcacgc
    17101 agcgcttcag ctcgattggc gcccgcgcaa agacatcgca cttatctcgc ggtttcgcac
    17161 gctcgatggc gagctcgcga acgagtatgt gcagctacgc acgcgctaca agctcgtgtc
    17221 gaacctcgtc ttcgactaca gccatcgcca ctcacgcgat tggcgttggg atccgtcgat
    17281 cacgagcaat gattcgatga cggcacgacg ctacttggat ctcgggccga acatcccgca
    17341 gtcgatcatc tcgggacgcg ccggtacgct gatcaaagaa aacgtcgacg tcctcgtgcg
    17401 cggcgctctc gcgtgggatg gcgccaacag cgacatcgag cgcaacacgt tcatggcgcg
    17461 ctacttcgag ctcggcaccg cgctcgaggt ccgcctccgc cgcaccgtcg gtgttggcct
    17521 atctgcgctc acgcgtcaga ccgaacgcac cgcgacggtc accggcgaga tcgtcgacat
    17581 cccgaatcag atcgatccga ttccgatccg ctacagcccc gagatgggcg agatcggctt
    17641 caccgagctc ggcacgacat tgcgcctctc cctcggtgcg cgccggctat ccgcgctcgt
    17701 cgaagcgtat gggcgacgca cgcgctacgc gctcgattac tgcgcgcccg cgatccagat
    17761 ggacggcacg tacgatccgc ggtgcatgtc cgcgctcgat accggcatcc agagccgaga
    17821 catccgcggt ggcggccggt tcacgctcga tgcctgggtg ggatcgcagc tgcgtctgtt
    17881 cgcgagctac gagatctcca gccgtatcga cttccagcgc gagctcagtg gcttcaagag
    17941 ccttcgcctc gtgatggagg gcaactacta gtgcgcgcgc tcgtcgtgct gctgctgctc
    18001 accggtgtcg ccgccgccga agcgccgttt ggcttcgacc acgtgattca cgcgcgcgac
    18061 gtcgacgtga agggcatgga cgccatcgcg tgcacgcggt gtcatgccga gaagcagggc
    18121 aagctcgtcg gcaaaccgac tcacacctcg tgtttcggcc agtgccatgg cccgctgccg
    18181 aaaccaccgg cgcgtggggg caagctcgca gagggggacc gcacgcgcat ctgccagagc
    18241 tgtcacagcg atgcgcagct caccgcgaaa tattccggaa agctccccgc cagctatccg
    18301 ccgttcacga tcgaccccga cttcggcgtc acgtttggtc acaagcaaca cgcggccgtc
    18361 acctgcgtgt cgtgtcacga cgtccgcgac aaaccggcga agggcgccgt gcacgcgcgg
    18421 tgcgctagct gtcacgatgg ttctggtgcg cgaggccgcg gcccagcgat ggcgaagtgc
    18481 acgacgtgcc accctgcagg cgtcgggagg ccgcagccgc cgcagctcgc cgtcgtgaag
    18541 gacagcgtcg aatcatcgtt ctcgcacgcc aagcacgcga cgcgcggagc ccaaggcaag
    18601 gactgcggca cgtgccacgc tacgatccga gagacggacg acttccagct accgcgaccg
    18661 acggtgaaga cgtgcgcgct cgccggttgc cacgacgcga aggctgcgtt tgcgacgacc
    18721 gtcgcgtgca cgcgttgcca cgatcgcgcg ccgaccgatc actttgaagt ctcgcgacct
    18781 accgcgcgct tcgagcatgc cggtaaccac gagcagctcg tgaagtcgac gccgtgcgcg
    18841 acgtgtcatc ccgtgttgcc gagcggcgaa gtgcgcgtcg tcggccacgc cccctgcgtc
    18901 gagtgtcaca gcgaggactt caccgcgcgc aggccacaga cctgcggcgc atgccacaac
    18961 gccaccgagc cgtggcgcaa gctcgtcgcc gatcgggcac cgccgcggca gaccgagttc
    19021 ggcgcatcgc tcgatcatac gaagcacaag gcggagtgcg cgacgtgcca cacgcttcgc
    19081 accgaaactg ccgagctgcg cacgtcgcgc ggacatcacg cgtgcacggg caacggctgt
    19141 catgcgccca aaggcggtgc ggcgccgcag atcaccgagt gcgcagcgtg ccatcgcatc
    19201 gggctctcga tcgagcggct gacggcgcgg atcagcgcag cctggtcggt gcgcgcaacg
    19261 ttccagcacg cgacacatcg cacgaccaag aacggcaacg acctcgcgtg cgccgcatgt
    19321 cacgaggatc tcgccggtga cctgctcgcg acgccgacgc caaagaaggc cgcgtgcatt
    19381 gcgtgtcacg acacgaacaa gcaagcgttc aaggtcaccg gcacgacctg ctcgcgttgt
    19441 catggagcac gcacgcgatg agcgacggga tgccacgctg gcagcgggcg tacatcctcg
    19501 cgacatgcgc gctgatcggc ggtgcgttcg cgtatgccgc atgcgactgg ggacgctggc
    19561 ctcgcctcac ctatcgtccg ctcacgggcg agatcatgat gaagacgccg ccgggcgcga
    19621 tcacggtcaa ctacctgggc accgtcgcgt ggggactcgg cggtgcggcg gcaggtgcgc
    19681 ttgtaggtgc gctgctgtgc gcgatcttca agcgaccttg gccgggccgg gcacttcacc
    19741 tattcggcgc gtgggcgcta actggcgtag tcctcgccgg cagttactac acgtggaact
    19801 tgtggccgtg gtgagactgt aagcacgtcg cgttgccctc gtttataggg cgtatgacga
    19861 cgaagctcgc cgctgccgtg ctcctcgctc tgacgtcgac cgccgctgcg caaacgtacg
    19921 tcgcgcgcga tgtcagctat gcggagccgc ccgctccgaa agctccgcgc atggtcgatg
    19981 cacggcttgg cctgatgctc ggtggctccg acgtcggcga tgcagatgga ttctccgcag
    20041 gcgcatccgg tgctctcggc taccgcatcg gcgacgtcac gctgcgcggc atgttcgatt
    20101 actacaaggt cggcgacggc tcggatgaag cactgtcgcg tcatggccgc gcgacgcgcg
    20161 tcggcggtgc agtgcgctac tcgttcgcga acaccggcta cgattcgagc gcagccgtcg
    20221 atttctgggg cgagctcggg gccggctacg agcacgtcgc gtggcgacac ggcggcattc
    20281 tcgagcggcc gagcggcgag cttgcagtcg gtctggatct cggacgtcgc ggcgagcgca
    20341 atgcacgcgg cgagcgaaaa gaaattggct acttcatggc gttccgcagc ctcgtcgcgc
    20401 acggccccga gatggagggc gcgaccgcga tgtgcggtgg tccttgcacc gaagcgacga
    20461 agccgccgcg caccgacgtc agcatgttct tcgagctcgg cgtgcactgg ggccgctgag
    20521 catcacgtaa ccgaaagggt cttcacatgc agatcgcgaa tggggtgctc cggcactcgt
    20581 tggtcccgtc ttgtctcgcg ttcctcgcag cctgcaccgg cggcggagga atgcagaagt
    20641 ccgctgtcta ctccggcggc ggtggcaccg ctgctgctcc gtcatcgacg atcgccggcg
    20701 attcatcgta ttcgccgccc gcccacgccg agacgtacta cgagcccgcg cgcccgtcgc
    20761 agcagtcgac gatcgtgcaa aacgatccgg cgccggagcg ccctggcctc ggcacgacgt
    20821 tcggcgagga agtctacgcg ccgatcagct tcgcgccgtt tcagcgttcg tcgggctcac
    20881 cgtgggcgga ggtcgcgctg cactacaacg acgccgacgg cgtgaacgcg cacgcgcagt
    20941 acctcggcgt cacgccgcag cccctcgaga tctacgcagg cgacggctcg atcgcggttg
    21001 ctctcgtcga cgagatgggg cgcacgctgc ccggcgtcga tgcaggcggc cgcacgctca
    21061 tcatgggtga ggacggcgcg cactatcgaa tcgtcgtgcg caacgcgacg acggcgcgct
    21121 tcgagatcgt ggcgtccgtc gacggtctcg acgtgatcga tggcaagcct gccgatccga
    21181 atcgccgcgg ctacatcgtc gacccgcacg acaccttggt gatcgacggc ttccgcacgt
    21241 ccgacgcgaa cgtggcggcg ttccggttcg gccgggtcgc agattcgtac gctgcgcaga
    21301 cgtc
//
LOCUS       DATLBZ010000447        18284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7731468, whole genome shotgun sequence.
ACCESSION   DATLBZ010000447 DATLBZ010000000
VERSION     DATLBZ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18284
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7731468"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1932)
                     /locus_tag="VMZ53_22610"
     CDS             complement(<1..1932)
                     /locus_tag="VMZ53_22610"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HUS31328.1"
                     /translation="MSTTLRLLAFALIVAVTACGPSGSGTIDSGGGLRTDAPPYMPDA
                     FGGPDFGQLCTTSDDCGDGGYCVESQDGDICTYSCDSGCPDYYHCRATDIGGKLVSLC
                     LPLRFEYCKPCTEDAACDGGICVQLEGGGYCLPPCPFEGTCPSGYSCGTDPTNAHQGS
                     YCVPNTGSCTCNAMNAGQVRTCSNMNGSGTCYGTETCMPNNGGWSGCNATSASSEVCD
                     GLDNDCDQLIDDGVSGSACPITNSNGTCQGVALCTGTGLTCQGQSPTAESCNFADDDC
                     DGMKDELWPTLEQVCSAGVGGCERFGVVRCNTAGNGVECSVTAGASQNEACNGLDDDC
                     DTQTDENFKSTFVTPPANPLGATCSVGVGACARTGNFVCAANGNGTACSVTPGTPSAS
                     ETCNGLDDNCNSQVDEGFKNQGTGQYDQNTACGSCSVDCTQLYALPNATGVCSTAGGT
                     PQCQMQCLPNAFDLDLASSNGCELVLDPNAVYVSTDDTNGADDANCGKGPFNTGSGNY
                     PCLTIAQGIVRAGQLGRTTIRVANGIYDEAVTIPNGVAMLGGHRPDNWQRDVAGTGTM
                     ITGVGTIATTTHDYTVRAVSITSATMFEGFLVIGSVNSKTGGNSYAIYVSGASSSLTL
                     RSNIIFSGRGGPGSTGGVGG"
     gene            complement(1929..4907)
                     /locus_tag="VMZ53_22615"
     CDS             complement(1929..4907)
                     /locus_tag="VMZ53_22615"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HUS31329.1"
                     /translation="MGRPLSCAVLVALAIGCGNPSRSPIDSNDGGFNVDAPPEPGQFG
                     GACSSHTDCESGYCIEPVGAHGGVCSRTCNDDCPQDWECRAVDLPQADVKVCVPAAKQ
                     LCLACQNDTECGGGACLTIDGGGRCAIPCSGQCPTGYTCKADASGSHTGNWCQPVTDS
                     CSCSAQLDGAMRSCSNMGTVNGNAVTCFGTQVCSAATGWSTCTANTPASETCDGVDND
                     CDFVIDDGVSTGASCNITNANGTCVGIQQCNGSSGFTCVGQTPAAEKCDAIDNNCNGG
                     VDETFVAAGLGSSCTAGVGACIRYGSQRCNTAQTGLVCSAVAGSPSAELCNQIDDNCN
                     GTVDEPFKTGAAALGTVCSAGLGVCTQYGSYVCATNGASTTCSVTPNTAAMTTETCNY
                     ADDNCDGVVDNGFRNTTTGFYDTDQNCGICGTNCTTMFTGNQAFGQCVVASSAQCVMR
                     CNAGYGNLNGSTIDGCEFQIDATAIYVSVADTTALDDATCGLGPTGTGTGNHPCKSIS
                     QGLLRSMTTGRVQILVADGTYSEGIVLLNGKQVKGGYRADTWERHVATTNTVIAGANV
                     SGVHDRTVTAASITSATLFEGFVVRGSFNAKPSGNSYAIYISGSNANLVIRDNQIFGG
                     RGGSGAAGSPGSNGPAGINGAAYAAATYDSFQTNGNNNLACDGTTNNRAAYGGGATAC
                     SGDVTSGGNGGGNNCTPSRSTQNSTATSPATAGQPGAGALGGAGGAVGTRGYDADLNQ
                     NTCVIPVQGQTGLPMFGTDGSAGSSGLPAAGVGGCSAAGGSVVGNEWVGGGGSTGQIG
                     GNGGGGGGGGAGGGATCTNCKRQGNFFVDAPDLLGGHGGGGGGGGCGGAGGAGGNPGG
                     GSFGIFVVGGSAPTITTNSITLGTGGIGGSGGIGGGGGLGGTGTQGGDQGTATVLFCT
                     GKGGRGGDGGDGGPGSGGGGGCGGSTFGIFTSGVGTPNYCASNSTTGGAIGTGGAGGF
                     SGGNSGGAGASGVLQNCVSL"
     gene            5005..7827
                     /locus_tag="VMZ53_22620"
     CDS             5005..7827
                     /locus_tag="VMZ53_22620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31330.1"
                     /translation="MGNSPEPDADLSAAPATLDSLERLLESGSGGAGYPVFAPATVLA
                     DRFVIEKLLGLGGMGAVYLARDRRLERQVALKLHRDDAAAERLHREAIAMAQLAHPNV
                     IPVFEVGTVENRSFVAMEYIEGSTLRAWLDEGSRSRSQRLDVLLAAGEGLAAAHDVGL
                     VHRDVKPENILVGRDARVRIGDFGLAQLTPPTTRPVRVPIGPETATGTVIGTPAYMAP
                     EQIDGRDVDARTDQFSFCVVAWEILVGRRPYSADDTLALRDVITRAEPAGARELPAAL
                     RGVLERGLATEPEQRWPSMHALLAALRSARKRPRRLAVAGAALAAAGLVATAALWPAR
                     DAGATCDATISSFDRELPTELTRSLGERIRSSGSPHADGRADVLLRTLDAQRTSAHRI
                     TLTACRAHANHQWSPDLVHASNECLADHARTVRELVTGVPVTRDSIADVLIVASRLPD
                     AAPCGDARLLAGWRPLSTNRALVGDVVAARGKLESAIAQADLGRLSAASTILEDVRNS
                     NVAKEPAVQGRLALLASTLHIAKGELAEAERSLTDAYFAARASDDGELVLRTVGELIR
                     LAANTRRDTASADRWVRDGLADAERERARYPVQAANVYLRAASAASIAGEAEVALERA
                     EKSRALLGDKLAPIMRGGYLAVRADAYASLGNITESLAASDEYLELLRQGLGDSHPAI
                     AEALADRAATLLEGERADEAATTAREAKAILDGEGQASTISVASVELSLGATLLQLGD
                     PTARDYLERSRDTFVHTYGENHPDVALADTNLALVYLDAGDGAKALATLRRAVSIQEH
                     VLGADHTELAAALYNLAVAERDGNDLDSALATARRAAAIFDKRQHGAVRHLYSLALVA
                     MIANMRGEHADALIAAEAALALPGDPEEPIGPAWAKLEAARALIKLRRDSARSQSLLA
                     EARAAYAAENMADRVAEIDGLRAGTR"
     gene            7845..8447
                     /locus_tag="VMZ53_22625"
     CDS             7845..8447
                     /locus_tag="VMZ53_22625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS31331.1"
                     /translation="MSDPAEVELRAFVAEGKLELAAARAIEQYGSELLGFLHARARND
                     DLAAEAFSVTCEQLWRRFASFRWEASLRTWLYCLARNALVTLQRDPRRDPARNLPLSV
                     LSSIEAVRRQSTEPYQRTETKQALRDLRDSLPAEDHELLLLRIDRRMSWKDIARTLAE
                     DEPTDSVLTSRAAALRKRYERLKEQLRELAIERGILDDVQ"
     gene            complement(8441..15478)
                     /locus_tag="VMZ53_22630"
     CDS             complement(8441..15478)
                     /locus_tag="VMZ53_22630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HUS31332.1"
                     /translation="MKTDAPIFMPGSGGVTDTGQATYSIPLWVPEGPQSAQPNLELTY
                     RGRANGSLGVGFALSGLSSIAPCTKTFASEGFADGIDFGQPGEAGLDQQDIRDSYCLD
                     GKKLVELASPKDSFPLGLNEKAFRTEVESFEHVVAHLPSSDAKVPPDQFIVYQRNGST
                     RLYKPVYASRLRPINGAVTREEVTAAVPIIYLLSEVTDRHNNRVVYKWQGTDAYNTHE
                     VAFQIGTIEYSFVDGTPRRRIEFSYETRTDSTVKYVSGVRVVNRSRLTRIDMYAPNPT
                     TTAKVWSYQLAYKLSGETGRSLLLNVKLCDVDNICSWVRSFDWREKRSFSTDLTSLSE
                     SEFDKVALGYDEESLEWYRSYTKANWLRPGDTRILLFDIDGDGDDDALYRRKPTYVDP
                     YSKSGSDDELHSGQLVDYRASAGDIEARLSSATEPLSMKKVSLTDILEPGLVDQGKPP
                     AGKTFAVANLGKSRIADMDGDGYLDLVLAKTRFTVSGARHEGDPYRRDDWSYGYSVQY
                     GGPWDHVNGVPFEAGKNLQLDAVAMKGPVMRYLAELQPKTQFFAWQPPPFQWTAADAD
                     GDGHAELVGPFVTTPGDPNPPTPIDVSQNYEMDWFDAKTMNGAGYGYRTFLSTDGQLA
                     SFGQPWVCGNGNAVVNDVDGDGRQDVLVAGDTTAPGETDPVTSSVSTFQRLTLADPWL
                     GAPPHGGAAKAGTSALWAGGCVSSAPDLVWGDWNGDGLTDVLYPPHQYGNNDKPVVRW
                     NYGTGFGAAEPIVIEGAPELNDLLDQKLPMGRFEPVQWDRGTRVADVDGDGRSDILAF
                     RQDNDACLNGVMSGLVQPDNFEGCMQKLIVYRSRGSRFVAEEIFSWGSGMFSFQHGFS
                     TAQIGDVNGDGAVDAVTVANHRLQVLELPWRAAPDVIENVNDGSTPYALEQFDYTHDW
                     WGDAPRAEATAFEVDGPHACAWPIACPRGGFEVVRQHRSFAGTKPDGSAMYRTQIHTY
                     ANPKISLIGRGSLGFAQHTIWDRESGRYTDRHFDNDVEVDTNPAWFGGTFYPYAGTAT
                     FETTTTPLKSVPSDADLQAATAAPGLASFTNYEVHTTWTSKLFDKKFSSDGRMLTILP
                     SSITVDDIENTVQANVSSKTPSYGVGSPFGSGSSTTTRLLHDDYGHITSQVTDISDGQ
                     LNALQVTRSTTTEYQTPRIGTKLWHVDLPSVVTQKSFDRDDSIAPARVTRVSYDSTTG
                     DVTSVKVAATGPAYPVCITQYAAESCETLGNVTYYTSRDSFGNPTYITTKAADVTTNR
                     TTRISWDGDGVYPMTVTNPLGHQTTTKIHPAYGVPIAVTDELGITSLARYDGFARLRS
                     ATRPGAAGLTRSYSPVAVGTRRGLRIDEAHDDGKLSYARVDELGRTIESGRRGFDAAT
                     WSYEQTTYDVLGNVAQISVPSTSLSNPKWINNNYDRLGQLTATYEANLARTTYKPRLL
                     STTTTDPEGHISYVNRDPAGRTVESGHLVGGAPYGAVKFGYSAFDQTESVTDAKGFVT
                     RLVHDELGRLATYNDPDAGLTTYRNDGYGQRTGETRANGDQYVRTYDVLGRLGDSTGP
                     DGITKRVYDTGTNATGRPTRVESADGVVTSISYDSYGRPYGIVQTVNGISDSIYHRYD
                     TYGRLKYLFYPVVSGQNRLTVSYNWANDGYLHSIADVSSCGLTPSGQLESAGASTCIG
                     TSLWKIDSRDAFNHLVHTTFGNGIAEDRAYDPLTGRITSHNAGGTTTTYTFLRDGQLK
                     TRRGENSRSESFQYDDLHRIQQWTLSWMDSTKVIRKSITDYSIDELGNLYNVATDGTT
                     TFSATFATAGRPHRMNWSSVGGTHKYDGNGRNYSGGGRTITYAENDLPAQIVEGSTTR
                     DFLYDGTGGRVMRSDATSTVRYMGKLFEHRTSTVSGTTDVAYVYGENAAIAQIDMTGT
                     RKATRYLASDPLGTVSVVFAGPLGSQVVDEIAYYDPYGKRISENGTAIVDPDKSTSRG
                     FGGHEHDTSDLVNMSGRIYDRKQFRFLTPDPVIGSPFFGQAYNPYSYVLNNPVNLTDP
                     SGFDPEGGGGAEGAGADASYNDPLDEGSIGVEEAPGNVFNVTIIGVPPHSSDASMGST
                     ATANVIQEEPWQPDMPISDAVRYGAGVAMDGNQSPIVRLLAGAGALMWVPFAINEHGA
                     AGIVSTPHAIVTGLDASMQHQQRAAALDAVGADGAALDERLQMAGALALALADIAGLV
                     TDISPSTEVTMVAKEAEAAEASIQHLLQFVPDAEYIARHYNPKQYVRPGHLFEEAKIA
                     KATKLAADSNANGFKSLREQFLYAIYADNGRLLKYGTSKSPMTRYTPEKFYKMFGDGA
                     YMELLDKGGTRVMRHGEQRLIRGHEFINGSKPPWNKAYH"
     gene            complement(15662..18250)
                     /gene="ligD"
                     /locus_tag="VMZ53_22635"
     CDS             complement(15662..18250)
                     /gene="ligD"
                     /locus_tag="VMZ53_22635"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HUS31333.1"
                     /translation="MSDPKEPGALEAYRAKREGGATPEPMASTVNATARGPRMFVVHQ
                     HDATRMHWDLRLEIDGVLCSWAVPKEPSMDPDDKRLAVKVENHPLEYVHFEAVIPPGN
                     YGAGAMIAWDRGLFRPLIDPAQGMIDGEIKFELYGYKLRGAFTLVHTGKGTRGKGSGR
                     GSNDWLLIKKRDEHAEAHMRSGKPLSAASVLSGLTVEELEARAPRHQAILSALADAKI
                     PKRAIAPGSVEPMLCQTADAAFSSADWLYELKYDGFRMMAHGGAGEAKLRYRSGLDPT
                     DRYPEITSAVRALPIGDMLLDGEIVMLDAAGKPDFHKLSFRGQMHRASEIQRAALASP
                     VTYIVFDLLACGGHDLRNVPLIERKALLRRLLPDVGPLRYSDHIEQHGEALLQQIVAH
                     GMEGVVAKKKSSVYKSKRSPDWLKMKADPEADFAVCGFTPPKGSRTGLGALHLCVWHE
                     GRWMWAGKVGSGFDDALLASLTAELTKKPRWRPTFPRPEGSGDALWIEPELVVQVRYR
                     EWPEDSSLRFPVFERLRRDKLPIDCPMPMRRTGEAPAPKEPGPGDSMEILVDEDPASS
                     RELRLSNPKKLLWKDENITKGELIEYYRSVAPYVLPYLKDRPTVLHRYPNGIDGEMFY
                     QKDMPDWSPPWLRTTTLWSNHSQREIHYLLLDDPDGLAFVANLASIPLHCWASRIADL
                     EKPDWTIIDLDPKNAPREHVVPLALAIHRLCDDIGLPNFVKTSGQSGLHVLIPLANQC
                     TFDQARTLAYLISLIIERQHPTMATTARNPAARGGRVYLDWGQNAHGQLLVAPYSVRP
                     VAGAPVSMPLVWDEVVPDLDPKQFNLRNALDRITSWASDPVLPVLSTKPDLAAALAAL
                     DKYARD"
BASE COUNT         2984 a         6012 c         5896 g         3392 t
ORIGIN      
        1 gccgccgaca ccgcccgtcg aaccggggcc gccgcgaccc gagaagatga tgttgctccg
       61 tagagtgagg ctcgacgacg cgccgctgac atagatcgcg tacgagttgc cgccggtctt
      121 cgaatttacc gagccgatga cgaggaagcc ctcgaacatc gtcgcgctcg tgatgctaac
      181 ggcgcgcacc gtgtagtcgt gcgtcgtcgt cgcgatcgtc ccgacgcccg tgatcatcgt
      241 gccggtgccc gccacatcgc gctgccagtt atcggggcgg tggccgccga gcattgcgac
      301 gccgttcggg atggtgacgg cttcgtcgta gatgccgttc gcaacgcgaa tcgtcgtgcg
      361 gccgagctgg cctgcgcgca cgatgccctg cgcgatcgtg aggcacggat agttgccgga
      421 ccctgtgttg aacggaccct tgccgcagtt cgcgtcgtcc gcgccgttcg tgtcgtcggt
      481 cgagacgtag accgcgttcg gatcgagcac gagctcgcag ccattcgacg aggcgagatc
      541 gagatcgaat gcattcggca ggcactgcat ctggcactgc ggcgtgccgc cggccgtcga
      601 gcacacgccg gtcgcgttcg gcaacgcata cagctgcgtg cagtcgaccg agcagctgcc
      661 acacgcggtg ttctgatcgt actgaccggt gccttggttc ttgaagcctt cgtcgacttg
      721 gctgttgcag ttgtcgtcga ggccgttgca ggtctcgctc gcgctcggtg tgccgggcgt
      781 caccgagcat gcagtgccgt tgccgttcgc cgcacacacg aagttaccag tgcgtgcgca
      841 cgcgccgaca ccgacgctgc atgtcgcgcc aagcggattt gccggcggcg tcacgaacgt
      901 cgacttgaag ttctcgtcgg tctgcgtgtc gcaatcgtcg tcgaggccgt tgcaggcttc
      961 gttctgcgac gcacccgctg tgacggagca ctcgacgccg ttgccggcgg tgttgcagcg
     1021 aaccacgccg aagcgctcgc agccaccgac gcccgcgctg cagacttgct cgagcgtcgg
     1081 ccacagctcg tccttcatgc cgtcgcagtc gtcgtcggcg aagttgcacg actcggccgt
     1141 cggggattgt ccctgacacg tcagtccggt tcccgtacaa agcgcgacgc cctgacaggt
     1201 gccgttgcta ttcgtgatcg gacacgccga accgctgacg ccatcgtcga tcagctgatc
     1261 gcagtcgttg tccaagccgt cacagacttc actgctcgcc gacgtggcgt tacagccgct
     1321 ccagccgccg ttgttcggca tgcaggtctc ggtgccgtag caggtaccgg acccgttcat
     1381 gttcgagcac gtccggacct ggcccgcgtt catcgcgttg cacgtgcagc tgcccgtgtt
     1441 gggcacgcag tagctgcctt ggtgggcgtt ggtcggatcc gtcccgcacg aatagccgct
     1501 cggacacgtg ccttcgaacg ggcagggcgg taggcagtag ccaccacctt cgagctgcac
     1561 gcagatgccg ccatcgcacg ccgcgtcttc cgtgcacggc ttgcagtact cgaagcgcag
     1621 cggcagacac agcgacacga gcttgccgcc gatatcggtt gcgcggcagt gatagtagtc
     1681 ggggcagccg gagtcgcagc tgtacgtgca gatgtcgccg tcctggctct cgacgcagta
     1741 gccgccgtcg ccgcaatcat cggacgtcgt gcacagctgt ccgaagtccg gaccgccgaa
     1801 agcgtcgggc atgtaaggcg gtgcatcggt gcgaagcccg ccaccgctat cgatcgtgcc
     1861 gctgccggaa gggccgcacg ccgtgacggc gacgataagc gcgaatgcga gaaggcgaag
     1921 cgtcgtgctc atagcgacac acagttctgg aggacgccgc ttgcgcctgc gccgccggag
     1981 ttaccgccgg aaaatcctcc tgcgccaccc gtgccgatcg cgccaccggt cgtcgagttc
     2041 gacgcacagt aattcggagt gccgacaccg ctcgtgaaga taccgaacgt cgatccgccg
     2101 cagcccccgc cgccgccgga gcccggcccg ccatcgccgc catcgccgcc gcgaccgccc
     2161 ttgccggtgc agaacaagac cgtcgccgtg ccttggtcgc caccttgcgt acccgttcct
     2221 ccgagcccgc cgccgccgcc gatgccgccg ctgccgccga ttccgccggt accgagcgtg
     2281 atcgagttcg tcgtgatcgt cggagccgag ccaccgacga cgaagatgcc gaacgagccg
     2341 ccgcctgggt tgccgccagc accaccggcg ccgccacagc cgccgccacc accgccgccg
     2401 ccgtggccgc caagaagatc cggcgcatcg acgaagaaat tgccttggcg tttgcagttc
     2461 gtgcacgtcg caccgccgcc tgcaccgcca ccgccgccgc caccaccatt gccgccgatc
     2521 tgaccggtgc tgccaccgcc gcccacccat tcattaccga cgacgctgcc gccggctgcc
     2581 gagcagccgc caaccccagc cgcaggcaat ccactgctac cagcagagcc gtcagtgccg
     2641 aacatcggca agcccgtttg gccctggacc ggaatgacgc acgtattctg attgaggtcc
     2701 gcgtcatagc cgcgtgtgcc gactgcaccg cccgcaccac cgagcgcacc tgcgccaggc
     2761 tgaccagcag ttgctggcga cgtcgcggtg ctgttctgcg tcgaacggga tggcgtgcag
     2821 ttgttgccgc cgccgttgcc gccactcgtc acgtcaccgc tgcatgccgt cgccccgccg
     2881 ccgtaggccg cacgattgtt cgtcgtgcca tcgcacgcga ggttgttgtt gccgttcgtc
     2941 tggaacgagt cgtacgttgc ggccgcatac gccgcgccgt tgatgccggc agggccgttc
     3001 gaacccggcg agcccgcggc acccgagcca ccacggccac cgaagatttg attgtcgcga
     3061 atcacgagat tcgcattcga gccgctgatg tagatcgcgt acgagttgcc gctcggcttt
     3121 gcgttgaacg agccgcgcac gacgaagcct tcgaacagcg tcgccgacgt gatcgacgcg
     3181 gccgtcaccg tacgatcgtg aacgcccgag acattcgcgc cggcgatcac ggtgttcgtc
     3241 gtcgcgacgt ggcgctccca ggtgtccgcg cgatagccgc ccttcacttg cttgccgttg
     3301 agcagcacga tgccttcgct gtacgtgccg tcggcgacga ggatctggac gcggcccgtc
     3361 gtcatcgatc gcaggaggcc ctgcgagatc gatttgcacg gatggttgcc ggtgcccgtt
     3421 cccgtcgggc cgagaccgca cgtcgcatcg tcgagcgccg tcgtatcggc gacggagacg
     3481 tagatcgcgg tcgcgtcgat ctggaattcg cagccgtcga tcgtcgagcc attgaggttg
     3541 ccgtagcccg cgttacagcg catcacgcac tgcgcgctcg acgcgacgac gcactgacca
     3601 aatgcttggt tgcccgtgaa catcgtcgtg cagttcgtgc cgcagatgcc gcagttctga
     3661 tcggtgtcgt agaagccagt ggtcgtgttg cgaaagccgt tgtcgacgac gccatcgcag
     3721 ttgtcatcgg cgtaattgca ggtctccgtc gtcatcgcgg cggtgttcgg ggtgaccgag
     3781 cacgtcgtcg atgcaccgtt cgtcgcgcag acgtacgagc cgtactgcgt gcacacgccg
     3841 aggcccgcgg agcacaccgt gccgagcgcg gcggcacccg tcttgaatgg ctcgtcgacc
     3901 gtaccgttgc agttgtcgtc gatctgattg cagagctcgg cggatggcga gcccgcgacc
     3961 gcagagcaga cgaggcccgt ctgcgcggtg ttgcagcgct ggctgccgta tcgaatgcac
     4021 gcaccgacgc ctgccgtgca cgacgagccg aggcccgccg cgacgaacgt ctcgtcgacg
     4081 ccgccgttgc agttgttgtc gatcgcgtcg cacttctcgg cggccggagt ctggccgacg
     4141 cacgtgaaac cgctcgagcc gttgcactgc tgaatgccga cgcacgtgcc gttcgcgttc
     4201 gtgatgttgc agctcgctcc ggtgctcacg ccgtcatcga tcacgaagtc gcaatcgttg
     4261 tcgacgccgt cgcaggtctc gctcgctggc gtgttcgccg tgcatgtcga ccaacccgtc
     4321 gccgcgctgc agacctgcgt gccgaagcac gtcactgcat tgccgttgac ggtgcccatg
     4381 ttcgagcagc tgcgcatcgc accgtcgagc tgggcgctgc acgagcagct gtccgtcacc
     4441 ggttggcacc agttgcccgt gtgcgagccg cttgcgtcgg ccttgcacgt gtagccggtc
     4501 ggacattgac cgctgcacgg aatcgcgcag cggccgccac cgtcgatcgt gaggcacgcg
     4561 ccgccgccgc actccgtatc gttctggcat gcgaggcaca gttgcttcgc tgcgggaacg
     4621 cagaccttca cgtcggcttg cggcagatcg acggcgcgac attcccagtc ttgcgggcag
     4681 tcgtcgttgc acgtgcgcga gcacacgccg ccgtgcgcac cgacgggctc gatgcagtag
     4741 ccggactcgc aatcggtgtg cgagctgcac gcgccgccga attgtccagg ctccggcgga
     4801 gcatcgacgt tgaatccacc gtcgttcgaa tcgatcggcg aacgggatgg attaccgcag
     4861 ccgatcgcaa gcgcgaccag cacggcacac gacagcggcc tccccacccg ccgacactag
     4921 ctattccctc gcgtgcttgt cgaggtcgaa ctgcctgcgt tgcgatggat gccgggacga
     4981 tgtgcgcctt atcattggcg cgaagtgggg aattcgccgg agcccgacgc tgacctctcc
     5041 gcagctcctg ccacgctgga ttcactcgag cgtctgctgg aaagcggaag cggaggcgcc
     5101 ggttacccag tgtttgcgcc ggctacggtt ctcgctgatc gattcgtcat cgagaagctt
     5161 ctcggcctcg gcggcatggg ggccgtctac ctagcgcggg atcggcggct cgaacggcag
     5221 gtcgcgctca agctccatcg cgatgacgcg gcagccgagc ggttgcatcg cgaagcgatc
     5281 gcgatggcgc agctcgcgca ccccaacgtc atccccgttt tcgaagtcgg caccgtcgag
     5341 aatcgatcgt tcgtcgccat ggagtacatc gagggctcga cacttcgcgc gtggctcgat
     5401 gaaggctcgc gatcgcggtc gcaacgcctc gacgtgttgc tcgccgcggg cgaaggactc
     5461 gctgctgcgc acgacgtcgg cctcgttcac cgcgacgtca aaccagaaaa catcttagtt
     5521 ggccgtgatg cacgcgtccg gatcggcgac ttcgggctcg ctcaacttac gcctccgacg
     5581 acgcgcccgg tgcgagtgcc gataggtccc gagacagcaa ccggaaccgt gatcggaaca
     5641 ccggcgtaca tggcacccga gcagatcgac ggccgcgatg tcgatgcgcg taccgaccag
     5701 ttctcgttct gcgtcgtcgc gtgggaaatc ctcgtcggtc ggcgcccgta cagcgccgat
     5761 gacacgctgg cgctgcgcga cgtcatcacg agagccgagc cggccggcgc tcgcgaactg
     5821 ccagcggctc tccgcggcgt gctcgaacgc ggcctggcta ccgagccaga acagcgatgg
     5881 ccatcgatgc atgcgcttct ggcagcgctg cgcagcgcgc gcaagcgtcc tcgacggctt
     5941 gcggtcgcgg gcgctgcgct cgctgctgcc ggcctcgttg ccaccgccgc gttatggccc
     6001 gcgcgcgatg ccggcgcgac gtgcgacgcg acgatcagca gcttcgatcg cgagctcccc
     6061 accgagctga cgcgctcgct cggagagcga attcgatcct cgggttcgcc acatgcggac
     6121 ggtcgcgcag acgttctgct ccgcacactc gacgcgcagc gcacaagtgc acaccgcatc
     6181 acactcaccg cgtgccgcgc tcacgccaac catcaatggt ccccagatct cgtgcacgcg
     6241 agcaatgaat gcctcgccga tcacgcgcgc acggtgcgag agctcgtcac cggtgtgcct
     6301 gtcacccgag acagcatcgc cgatgtgttg atcgtcgcgt cgcgacttcc cgatgctgcg
     6361 ccgtgcggag atgcgcgttt gcttgccggg tggcgcccgc tctccacgaa ccgcgcgctc
     6421 gtcggtgatg tggtagccgc acgcggcaag cttgagagtg cgatcgcaca agcggacctc
     6481 ggacgcctct ccgccgcgag cacgatcctc gaagacgttc gcaactccaa tgtcgcgaag
     6541 gagccggccg tgcagggacg tctcgcgctc ctcgcatcga cccttcacat cgccaagggt
     6601 gagctcgcag aagccgagcg ctcgctgacc gatgcgtact tcgctgcacg tgcgtccgac
     6661 gacggcgagc tcgtactgcg caccgtcggt gagctgatcc ggcttgccgc gaacacacgt
     6721 cgcgatacag caagcgccga ccgctgggtg cgcgatggtc tcgccgacgc ggagcgcgaa
     6781 cgcgctcgct atccggtgca ggctgcgaat gtctacctac gcgccgcatc tgccgcctcg
     6841 atcgcaggcg aagcagaggt cgcgctcgag cgtgccgaga agagtcgtgc gctgctcggc
     6901 gacaagctcg caccgatcat gcgtggcggg tatctcgccg tacgcgccga cgcatacgct
     6961 tcgctcggca acattaccga gtcgctcgcg gcatcagacg aatatctcga gctcttgcgc
     7021 caaggtctcg gtgacagtca ccccgcgatt gccgaagcac tcgcggatcg cgccgcaacg
     7081 ctgctagaag gcgaacgcgc agacgaagca gcgacgacag cacgcgaagc caaggcgatc
     7141 ctcgacggag aagggcaggc gagcacgatc tcggtggcgt cggtcgagct cagtctcggc
     7201 gcgacccttc tccagctagg cgaccccaca gcccgcgact atctcgaacg ttcgcgcgac
     7261 acgttcgtgc acacgtacgg cgaaaatcac ccggacgtcg cgctcgcgga cacgaacctc
     7321 gcgctcgtgt atctcgacgc cggcgacggc gccaaggcgc tcgcgacact gcgacgtgct
     7381 gtctcgatcc aggagcacgt gctcggtgcc gatcacaccg agcttgccgc cgcactctac
     7441 aacctcgcgg tcgcggaacg cgatggtaac gatctcgact ccgcgctcgc gaccgcacgc
     7501 cgtgcggctg cgatcttcga caagcgccag cacggtgctg tccgtcatct gtattcactc
     7561 gctcttgtag cgatgatcgc gaacatgcga ggcgagcacg ccgacgcgct catcgccgcc
     7621 gaagccgcgc tcgcactgcc cggcgatccg gaggagccca tcggtccggc atgggcaaag
     7681 ctcgaagccg cgcgtgcgct gatcaagctc cgccgagatt ccgcgcgctc gcagtcgctg
     7741 ctggcagaag cgcgtgcggc gtacgccgcc gagaacatgg cggaccgcgt cgccgagatc
     7801 gacggcctac gcgccggcac ccgctgacgg tgtacgctgc gcacgtgagc gacccggctg
     7861 aagtcgagct gcgcgcgttc gtagcagagg gcaagctcga gctcgcagcc gcgcgcgcga
     7921 tcgagcaata cggcagcgag ctgcttggct ttctgcatgc acgcgcccgc aacgatgacc
     7981 tcgccgcaga ggcgttctcc gtgacgtgcg agcagctgtg gcgccgcttc gcgagctttc
     8041 gctgggaagc gagcctgcgc acgtggctct actgcctcgc gcgcaacgct ctcgtaacgc
     8101 tccagcgtga tcctcgtcga gatccggcgc gcaacctccc gctctccgtc ctctcgagca
     8161 tcgaagcggt gcgccgccag agcacggagc cgtaccagag aaccgaaacg aagcaggccc
     8221 tgcgcgatct gcgcgactcg cttcccgccg aggaccacga gctgctcctg ctccgcatcg
     8281 atcgccgcat gtcttggaag gacatcgccc ggacgctcgc cgaggacgag cccacggact
     8341 cggtgctcac gtcgcgcgcc gcggcgctgc gtaagcggta cgagcgactg aaagagcagc
     8401 tccgcgagct cgcgatcgag cgaggaatcc tcgacgacgt tcagtgatac gccttgttcc
     8461 acggtggctt cgaaccgttg atgaattcgt ggccgcggat gagacgttgc tcgccatgcc
     8521 gcatcacgcg cgtccctccc ttgtcgagga gctccatgta cgcgccgtcg ccgaacatct
     8581 tgtagaactt ctccggcgtg tagcgagtca tcggactctt cgacgtcccg tacttcaaca
     8641 ggcgaccatt gtccgcgtag atcgcgtaga gaaactgctc gcggaggctc ttgaacccgt
     8701 ttgcattcga atccgcggcg agcttcgtgg cctttgcgat cttagcctct tcgaagaggt
     8761 gtccgggacg tacgtactgc ttcggattgt agtggcgcgc gatgtactcc gcgtcgggca
     8821 cgaattgcag caggtgttgg atgctggcct cagctgcctc cgcttctttc gcgaccatcg
     8881 tgacttccgt cgaagggctg atgtccgtca cgaggcccgc gatatcggcg agggcgagtg
     8941 ccagagctcc tgccatctgc aagcgttcgt cgagcgccgc cccatcggcc cccaccgcgt
     9001 cgagtgccgc cgcgcgctgc tgatgctgca tgctcgcgtc caggcccgtc acgatcgcgt
     9061 gcggcgtcga gacgatgcct gccgcaccat gctcgttgat cgcgaacggg acccacatca
     9121 gtgcaccggc acccgcaagc agacgaacga tcggcgattg gttgccatcc atggcgacgc
     9181 ctgcgccata acggacggca tccgaaatcg gcatgtcggg ctgccacggc tcttcctgga
     9241 tgacgtttgc cgtggcggtc gaacccatgc tcgcgtcgct gctgtgcggt ggaaccccga
     9301 tgatcgtgac gttgaagacg ttgccggggg cttcctcgac gccgatcgac ccttcgtcga
     9361 gtgggtcatt atatgaagca tccgctcccg cgccctccgc gccaccacct ccctccggat
     9421 cgaaccccga cggatccgtc aggttcaccg ggttgttcag cacgtagctg tacggattgt
     9481 aggcctgtcc gaagaatgga cttccgatga ccgggtcagg cgtcaggaat cggaactgct
     9541 tgcggtcgta gatgcgtccg ctcatgttca cgaggtcgga ggtgtcgtgc tcgtggccac
     9601 cgaaaccgcg cgaggtggac ttatcgggat cgacgattgc agtcccattc tcggagatgc
     9661 gcttgccgta cggatcgtag tacgcgatct cgtcgacgac ttgtgagccc agcggccccg
     9721 caaacacgac gctgaccgtg ccgagcggat cggacgcgag atagcgagtt gccttgcgcg
     9781 ttcccgtcat gtcgatctgc gcgatcgccg cgttctctcc gtagacgtac gcgacgtctg
     9841 tcgtcccgct gacggtgctc gtccggtgct cgaacagctt gcccatgtag cgcacggtgg
     9901 acgtcgcatc gctgcgcatc acgcggccac ccgtgccgtc gtacaggaag tcgcgcgtcg
     9961 tggaaccctc gacgatctgc gcgggcagat cgttctctgc atacgtgatc gtgcggccgc
    10021 cccccgaata atttcgtccg ttcccgtcgt atttgtgcgt tccgccgacc gagctccagt
    10081 tcatgcgatg cggccggccc gcggtcgcaa atgtcgcgga gaacgtcgtc gtgccgtccg
    10141 tcgccacgtt gtagaggttg ccgagctcat cgatgctgta gtccgtgatg ctctttcgga
    10201 tgaccttcgt cgaatccatc cacgacagcg tccattgctg gatgcggtgg aggtcgtcgt
    10261 actggaacga ctcgctgcga ctgttctcgc cacggcgcgt cttcagctga ccgtcgcgca
    10321 agaacgtgta cgtcgtcgtc gtgccgcccg cgttgtgcga cgtgatgcgg cccgtcaatg
    10381 ggtcgtacgc gcgatcctcc gcgattccat tgccgaacgt ggtgtggacg agatggttga
    10441 aggcgtcccg gctatcgatc ttccaaaggg acgttccgat gcatgtggac gcgccggcgg
    10501 attccagctg gccgctcggc gtcaatccgc aactcgagac gtccgcgatg gagtggaggt
    10561 acccgtcgtt cgcccagttg taggaaacgg tcaatcggtt ctgtccggac acgaccggat
    10621 agaacagata cttcaagcgt ccgtacgtgt cgtagcggtg atagatagag tcgctgattc
    10681 cgttgacggt ctgcacgatc ccatacggcc gtccgtagct gtcgtaggag atggacgtga
    10741 cgacaccgtc agccgactcc acgcgcgtcg gtcttccggt ggcgttcgtc ccggtgtcgt
    10801 agacgcgctt cgtgatgccg tcgggtccgg tgctgtcgcc gagtcgaccg aggacgtcat
    10861 aggtgcgcac gtactgatcg ccgtttgcac gcgtctcgcc ggtgcgttgg ccatagccgt
    10921 cgttgcggta cgtcgtgagt cccgcgtcgg gatcgttgta cgtcgcgagt cggccgagct
    10981 cgtcgtgaac tagacgcgtc acgaatccct ttgcgtcggt cacgctctcg gtctggtcaa
    11041 acgcggagta tccaaacttg acggcgccgt acggcgcgcc gccgactagg tgaccgctct
    11101 ctacggtgcg gcccgctgga tcgcgattca cgtagctgat gtggccttcg ggatcggtcg
    11161 tcgtggtcga gaggagtcgc ggcttgtacg tggtgcgagc aaggttcgct tcgtacgtgg
    11221 ctgtcagctg cccgaggcgg tcgtagttgt tgttgatcca cttcggattc gacagtgacg
    11281 tcgacggcac cgaaatctgg gcgacattgc cgagcacgtc gtacgtcgtc tgctcgtagc
    11341 tccacgtcgc cgcgtcaaag cctcggcgcc ccgactcgat cgtgcgaccg agctcgtcca
    11401 cccgcgcgta cgacagcttg ccatcatcgt gtgcttcgtc gatgcgcaga cctcggcgcg
    11461 ttccgacggc gacgggcgag tagctgcgcg tgaggccggc ggctcccggg cgcgtcgccg
    11521 agcggagtcg agcgaatcca tcgtagcgag ctagcgacgt gatgccgagc tcgtcggtga
    11581 ccgcgatcgg cacaccgtag gcgggatgga tcttcgtcgt cgtctgatgg ccgagtggat
    11641 tcgtaacggt catcggatag acgccatcgc cgtcccacga gatgcgcgta gtgcgattcg
    11701 tcgtgacatc cgcggccttc gtcgtgatgt acgtcgggtt tccgaatgaa tcgcgactgg
    11761 tgtagtacgt gacgttgccg agcgtctcgc agctctcggc ggcgtactgt gtgatgcaca
    11821 cggggtatgc ggggccggtg gcagctacct tgacgctcgt gacatcgcct gtcgtgctgt
    11881 cgtagctcac gcgcgtgaca cgcgccggcg cgatggagtc gtccctgtcg aacgacttct
    11941 gggtcacaac ggagggaagg tcaacatgcc agagcttcgt gccgatacgc ggcgtttgat
    12001 actcggtggt cgtgctgcgt gttacctgca acgcgttcaa ctgaccgtcg gaaatgtccg
    12061 tgacctgact cgtgatgtgc ccatagtcgt catgaagcag gcgcgtggtc gtgctgctgc
    12121 cgctgccgaa tggcgagcca acgccatacg aaggcgtctt cgagctgacg ttcgcctgca
    12181 cggtgttctc gatgtcgtcg acggtgatcg acgaaggcag gatcgtgagc atgcggccgt
    12241 cagaagagaa tttcttgtcg aagagctttg acgtccacgt cgtgtgtact tcgtagttcg
    12301 tgaaggacgc gagaccgggg gcggctgtgg ctgcttgaag atccgcgtcc gatggcaccg
    12361 acttgagcgg cgtcgtcgtc gtttcaaacg ttgccgttcc ggcatacgga tagaacgttc
    12421 cgccaaacca tgccggattc gtgtcgacct cgacgtcgtt gtcgaagtga cggtccgtgt
    12481 agcggccgct ctcgcggtcc cagatcgtgt gctgcgcgaa gccgaggctg ccacggccga
    12541 tcaggctgat cttcggattc gcgtacgtgt ggatctgcgt tcggtacatc gcgctgccat
    12601 ccggcttcgt tccagcgaac gagcggtgtt gacgcacgac ctcgaagccg ccacgcgggc
    12661 acgcgatcgg ccacgcgcac gcatgggggc cgtccacctc gaaagccgtc gcttcagcgc
    12721 gcggcgcatc gccccaccaa tcgtgcgtgt agtcgaattg ctcgagcgcg tagggcgtgc
    12781 tgccgtcatt gacgttttcg atcacgtcgg gcgcggcacg ccatggcagc tccagcactt
    12841 gcagacgatg attcgcgacc gtcaccgcat ccacggcgcc atcaccattg acgtcgccga
    12901 tctgcgcggt cgagaagccg tgctggaagc tgaacatgcc ggagccccac gagaagattt
    12961 cctcggcgac aaagcggctg ccgcgcgagc ggtagacgat gagcttctgc atgcagccct
    13021 cgaaattgtc gggttgcacg agaccggaca taacgccgtt caggcacgcg tcattatctt
    13081 gacgaaacgc gaggatgtcg cttcgcccat cgccatcgac atctgccacg cgcgtcccgc
    13141 ggtcccactg cacgggctcg aaccggccca tcggcagctt ctgatcgagg aggtcgttga
    13201 gctcgggtgc gccttcgatg acgatcggct cggcggcgcc gaacccggtg ccgtagttcc
    13261 agcgcacgac gggcttgtcg ttgttgccgt actgatgcgg tggataaagc acgtccgtca
    13321 gtccgtcacc gttccagtcg ccccacacga ggtccggcgc gctcgagacg cagccgccgg
    13381 cccacaacgc cgaggtgcct gctttcgcgg cgccaccgtg cggcggcgca ccgagccacg
    13441 gatccgcgag cgtcagccgc tggaacgtcg agacggaact tgtgaccggg tctgtctcgc
    13501 cgggagccgt cgtgtcaccg gcgacgagca cgtcctggcg cccatctcca tcgacgtcgt
    13561 tgaccacggc gttgccgttg ccgcacaccc acggctggcc gaacgaggcg agctgcccat
    13621 cggtcgaaag gaaggtccga tagccgtagc ccgctccgtt catcgtcttc gcatcgaacc
    13681 agtccatctc gtagttctgg ctgacgtcga tcggcgtcgg cggattcgga tcacccggcg
    13741 tcgtaacgaa cggtccgacg agctcggcgt gaccgtcgcc atcggcatcc gctgcagtcc
    13801 attgaaatgg cggaggctgc cacgcgaaga attgcgtttt gggctgcaac tccgcgaggt
    13861 agcgcatcac gggccccttc atcgcgaccg cgtcgagctg caggttcttg cccgcttcga
    13921 aggggacccc gttcacgtgg tcccacgggc cgccgtactg gaccgagtag ccgtacgacc
    13981 agtcgtcgcg gcgatacgga tcgccttcgt ggcgcgcccc cgagacggtg aagcgcgtct
    14041 tcgcgagcac gagatcgagg tatccgtctc cgtccatatc cgcgatccgc gactttccga
    14101 gattcgcaac cgcgaacgtc ttgcccgccg gcggcttgcc ttgatcgacg aggcccggtt
    14161 cgaggatgtc ggtcagcgac accttcttca tcgacagtgg ctccgtggcg ctcgagagcc
    14221 gcgcctcgat gtcgccggct gacgcgcgat agtccacgag ctgcccggaa tggagctcgt
    14281 cgtcactgcc gctcttcgag tacggatcga cgtatgtggg cttgcgtcga tagagcgcgt
    14341 catcgtcgcc atcgccgtcg atgtcgaaga gaagaatccg cgtatcgccc ggccgcagcc
    14401 agttggcttt cgtgtaggag cggtaccact cgagagattc ttcgtcgtag ccgagcgcga
    14461 ccttgtcgaa ttcgctctcg gagagcgacg tcagatcggt gctgaagctg cgcttttcgc
    14521 gccagtcgaa ggagcggacc cagctacaga tgttgtcgac atcgcatagc ttcacgttca
    14581 ggaggaggct ccgtccggtc tctccgctca gcttgtaggc gagctgatat gaccagacct
    14641 tcgccgttgt cgtcggattc ggtgcgtaca tgtcgatgcg cgtgaggcgc gaccgattca
    14701 cgacgcggac gccgctgacg tacttcaccg tgctgtctgt ccgggtttcg tacgaaaact
    14761 cgatgcgtcg ccgcggagta ccgtcgacga acgaatattc gattgtgccg atctggaagg
    14821 cgacctcgtg cgtgttgtac gcgtcggtgc cctgccactt gtagacgacg cgattgttgt
    14881 gtcggtccgt gacctccgac aggaggtaga tgatcggcac ggccgccgtt acctcctcgc
    14941 gtgtcaccgc gccgttgatg gggcgcaggc gtgatgcgta gacgggcttg tacaggcgcg
    15001 tgctgccatt gcgctgatag acgatgaact gatccggtgg caccttcgca tcgctcgagg
    15061 gcaggtgcgc gaccacatgc tcgaagctct cgacctccgt gcggaacgcc ttctcgttca
    15121 ggccgagagg gaaagagtct ttgggcgacg cgagctcgac gagcttcttg ccgtcgagac
    15181 agtacgagtc gcggatgtcc tgctgatcga gacccgcttc gccgggctgt ccgaagtcga
    15241 tcccgtccgc gaacccctca gacgcgaagg tcttcgtgca cggggcaatc gacgagagac
    15301 ccgagagcgc gaagccaacg ccaagtgagc cattggcacg acctcgatat gtgagctcga
    15361 gatttggctg ggcactctgc ggaccctcgg gaacccacag cggaatcgag taggtcgctt
    15421 gaccggtatc cgtgacgccg ccgcttccgg gcatgaagat cggcgcatcg gtcttcacga
    15481 tcgaaccgag cgggtaggtc ggcccgccgg cgatctgcgc atggacggga gcgacgtgcc
    15541 acgacgggaa cagctgtgcg attaacgtga cgatggcgcc cgccgcgacg gcgctacgcg
    15601 tatgggatcg aggcattgcc gatagacgcg cccgcgatcc gatgtgacag acttttttcg
    15661 ctcagtctct cgcgtacttg tcgagggccg cgagagcggc agcgagatcc ggtttcgtcg
    15721 acagcacggg cagcaccggg tcggatgccc aactcgtgat ccgatcgagc gcattgcgga
    15781 gattgaactg tttgggatct aggtcaggca cgacctcgtc ccacacgagc ggcatcgaga
    15841 cgggggcgcc tgcgacgggc cgcacactat agggagcgac gagcagctgg ccgtgtgcgt
    15901 tctgccccca gtcgagatac acgcggccgc cgcgtgcggc tggattgcgt gctgttgttg
    15961 ccatcgtcgg atgctgccgc tcgatgatga gcgagatcag atacgcgagc gtgcgcgctt
    16021 gatcgaacgt gcactgattc gcgagcggga tgagcacgtg caggcctgat tggcctgagg
    16081 tctttacgaa gttcggcagg ccgatgtcat cgcagaggcg atggatcgcg agagcgagcg
    16141 gcacgacgtg ctcgcgcggc gcgttcttcg gatcgagatc gatgatcgtc cagtcgggct
    16201 tctcgagatc ggcgatgcga ctcgcccagc agtggagcgg aatcgacgcg aggttcgcga
    16261 cgaatgcgag accgtccgga tcgtcgagca gcagataatg gatctcgcgc tggctgtgat
    16321 tgctccacag cgtcgtcgtc cgaagccaag gcggcgacca gtcgggcatg tccttttggt
    16381 agaacatctc gccgtcgatg ccgttcggat agcggtgcag caccgtcggg cgatccttca
    16441 agtagggcag cacgtacggc gcgacggagc ggtagtactc gatgagctcg cccttcgtga
    16501 tgttctcgtc cttccagagc agcttcttcg ggttggacaa gcgcagctcg cgcgacgagg
    16561 ccggatcttc atcgacgagg atttccatcg agtcgcctgg acctggctcc ttcggcgcgg
    16621 gcgcttcacc cgtgcgacgc atcggcatcg gacaatcgat cggcagcttg tcgcggcgca
    16681 ggcgctcgaa gaccgggaag cgtagcgagc tgtcttccgg ccactcgcgg tagcggactt
    16741 ggacgacgag ctcgggctcg atccacaacg cgtcgccgct gccttcggga cgcgggaacg
    16801 tcggacgcca gcgcggcttc ttcgtcagct ctgcggtgag cgaggcgagg agcgcatcgt
    16861 cgaagccgct gccgactttg ccggcccaca tccagcggcc ttcgtgccac acgcagaggt
    16921 gaagcgcgcc gaggccggtg cggctgccct tcggcggcgt gaagccgcag acggcgaagt
    16981 cggcctcggg gtcggccttc atcttgagcc agtccggcga gcgcttgctc ttgtagacgg
    17041 acgacttctt cttcgcgacg acgccttcca tgccgtgcgc gacgatctgc tggagcagcg
    17101 cttcgccgtg ctgctcgatg tgatcgctgt agcggagtgg cccgacgtca ggcagcaggc
    17161 gacgcagcag cgccttgcgc tcgatgagcg gcacgttgcg gaggtcgtga ccgccgcacg
    17221 cgagcaagtc gaacacgatg tacgtgaccg gagaggcgag ggcggcccgt tggatctcgc
    17281 tcgcgcgatg catctggccg cggaacgaga gcttgtggaa gtcgggcttg cccgcggcgt
    17341 cgagcattac gatctcgccg tcgagcaaca tgtcgccgat cggcagcgcg cgcaccgcgc
    17401 tcgtgatctc cggatagcga tccgtcggat cgagccctga gcgatagcgc agcttcgctt
    17461 cgccagcgcc gccgtgcgcc atcatgcgaa agccgtcgta cttgagctcg tagagccaat
    17521 ctgccgacga gaacgcggcg tctgcggtct gacacagcat cggctcgacg ctgcccggcg
    17581 cgatcgcgcg cttgggaatc ttcgcgtcgg cgagcgcgct caggatcgct tgatgccgcg
    17641 gcgcacgcgc ttcgagctct tccaccgtca gtcccgagag gacgctcgcg gccgacagcg
    17701 gcttgccgct tcgcatgtgc gcctcggcgt gctcgtcacg cttcttgatg agcagccaat
    17761 cgttgctgcc gcgacccgag cctttgccgc gcgtgccttt gcccgtgtgc acgagcgtga
    17821 acgcgccgcg cagcttgtag ccgtagagct cgaacttgat ctcgccgtcg atcatgcctt
    17881 gcgccggatc gatcagcggt cggaacaatc cgcggtccca cgcgatcatc gcgcctgcgc
    17941 cgtagttgcc gggcgggatg accgcttcga agtggacgta ctcgagcgga tggttctcga
    18001 ccttcaccgc aagccgcttg tcgtccggat ccatggacgg ctctttgggc accgcccagc
    18061 tgcacaacac accgtctatc tcgagtcgga gatcccagtg catgcgggtc gcgtcgtgct
    18121 ggtggacgac aaacatcctc ggtccacgcg cagttgcgtt gaccgtgctg gccattggtt
    18181 caggtgtggc tcccccttcg cgctttgcgc ggtacgcttc gagcgcgccg ggttctttgg
    18241 gatcactcac gacgaaccga tacaatttat tttatgcccg ctcg
//
LOCUS       DATLBZ010000448        10180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4269277, whole genome shotgun sequence.
ACCESSION   DATLBZ010000448 DATLBZ010000000
VERSION     DATLBZ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269277"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VMZ53_22640"
     CDS             complement(<1..899)
                     /locus_tag="VMZ53_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HUS31334.1"
                     /translation="MSTHGDTPRNEKSNGNGNGHHKKANGNGHSNGNGKTTVKVNTPD
                     QLNHRELLAALRAFKRGNFEVRMRDDLTGLDGQVAETFNELVSMVKQIKEESHDVCSA
                     VGKEGQAAKRMRRLGTTGGWADYIQSVNEVIEDLSGHANEIARVVSAVAKGDLEQTMD
                     VEDGGFGPRRGEFLRHARIVNGMVARLSQFGSEVTRVALEVGGEGKLGAQARVQGVSG
                     VWKDLTDSVNLMASNLTSQVREVARVTTAIAQGDLSKTVTIDVKGEILELKNTINTMV
                     EQLRGFANEVTRVAREVGTEGRLG"
     gene            complement(896..2581)
                     /locus_tag="VMZ53_22645"
     CDS             complement(896..2581)
                     /locus_tag="VMZ53_22645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS31335.1"
                     /translation="MQLDAPLHSDQLDDGEGEEDGALLSYGRDILVVDDNDTNLIAIE
                     AALLPLGRKMVLARSGVEALARLLEQDFALIILDVAMPGMDGFETARLVRSRARNRAT
                     PIIFITGLSWQDDAILKGYELGAFDFLMKPIRGEVLRAKAMVFIELQARTLELRDKAE
                     QLREAQTRAHERELTMQRKRFEAEVLERQVAQMAEVDRRKDEFLAILAHELRNPLQPL
                     QTAVEIMANTADKPVPERVRGIISRQVQHIGRLVDDLLDVARFQSGKLSLRKEHVPLD
                     AIITEAINDMRPVITGRKHVLEVHGIDASPVIHGDPMRLVQVLTNLLSNAVKYTDPGG
                     TIVVDWGIGEDKLAFIRVKDNGRGITDELLPRIFDMFVQERTTPDGKGGLGLGLGLVK
                     RLVELHGGTVSANSDGSGKGASFEVKLPVADTLQASRTQTKPDTEPHERRLRAVVCDD
                     APDLRELVADLLRMKGHEVTVVEDGPSAIRVISLEKPDVALIDIGLPDMDGYEVARQL
                     RRLMTDPVRLIAMTGYGQAADRATALQAGFDAHIVKPASADKIMRALYGKEEQ"
     gene            complement(2835..3638)
                     /locus_tag="VMZ53_22650"
     CDS             complement(2835..3638)
                     /locus_tag="VMZ53_22650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31336.1"
                     /translation="MRSVVVLLLTCGVAAAGPSFKDKKAKALADALNADLEHVIDVGS
                     DEIMDGGYRGKIHLVPELPEGKYRQQLVWVDKALRAIDDFFVGFDKPKYRWRKLDVHF
                     VRSVGKRTPSAYATGWSITHNVVGSLLTSERGVRETYFHELFHLNDFDHGDWSAKTLT
                     TDYDAIIKKCGAVGAKAVKCLAPFAPNDTKVKASGTYYAFQPNNGDAVHEYAAELAVR
                     YFKEQSEMAANGKLSRAAFKCGPPENRRAWKGIVDEFFAGHDLVPACSE"
     gene            3716..4420
                     /locus_tag="VMZ53_22655"
     CDS             3716..4420
                     /locus_tag="VMZ53_22655"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUS31337.1"
                     /translation="MVPRNRFLAALACGLPTIVVGVASADDYYPTDPGVAPPRSESLW
                     RDPSVASGVGIGVDVGGGVTGFVGSRLRSTTSSFGGAWSVRAAFGTHIPLALEVGYTG
                     SATAINGQLGPETATLMGRTLESAIRYTVMPRARWSPYAFAGLGWQHYTINDRSFELS
                     DTGIRTSDDLLVMPFGLGMGYRVGAIVTDLRGTLRAARGANLVLDNAEVALATGQGSY
                     APMHSWETSLNVGYEF"
     gene            complement(4417..5646)
                     /locus_tag="VMZ53_22660"
     CDS             complement(4417..5646)
                     /locus_tag="VMZ53_22660"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HUS31338.1"
                     /translation="MVARVLALVALLAACEKRSALYCDKNPEDLAHCPRTDGEVPVMM
                     CTDNASCTTSQRPFCELGSHVCVGCLSNADCTDPTKLNCDPTALECRGCVAHSDCPSS
                     ACLPGGVCGDDANVIYVDPAAAANGDGTKTKPVQTIADALDLVTATRKNIKLTGTLDE
                     TVLLMARDVVIVTDPGTKLLGKGDPAVKINASQVTAYGLDIDCSGAMNCLRVESSSTA
                     RLYRVNVHDAPKSGVELKDNSYLELDQSTVAGNFDGIITSASGTFSITNNFIIHNGSA
                     TSAHGGVDIGSSSPANRFEFNTVAHNQIKDGLILAGGITCPLASSVAVPNNLIVANTG
                     SLGNAANCVTTGSPPDADAVPYQFKSITPPYDYHLLGGSKAIDASTITSTITNDVDGD
                     FRPQGAKKDLGADEYKP"
     gene            complement(5628..6581)
                     /locus_tag="VMZ53_22665"
     CDS             complement(5628..6581)
                     /locus_tag="VMZ53_22665"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS31339.1"
                     /translation="MRALVVALFMLFAAPARAGTVGIVVTGDQGLQAELSKQLDNWLR
                     GHGHQVADALGADGTRSLLNCMEIDDQGCAQGVVAARAKTEAVLYGQAAKSRTSKATI
                     LTIYWLYKDKEPVGMRRACEDCTADVMRGIVDEMLGLVTQVSSEKRGRVALHSKPEGM
                     QVLIDNENIGVTPMERDLAVGTHQIVLMHRGQRVGERSLKVQPDVSAEITIPVTMPVD
                     DEPEGRRSRPSRVVPALVLGLGVAAIAGGAVLYVTSEEDTGEKLYYRDTKLAGIGVAA
                     GGVVVTALGTWLWVRAGGPADSAPVATLDAHGGTIGWSRAF"
     gene            complement(6578..8248)
                     /locus_tag="VMZ53_22670"
     CDS             complement(6578..8248)
                     /locus_tag="VMZ53_22670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31340.1"
                     /translation="MIRALLLLSLVAGCTETIRVQADSLDGLESLEVYPPDAVITVTD
                     LAPPEHTLQYIAIGRFSDGTTREVTSLVGWSVDNVRIGALDGNGLFTATQTAAGHGVV
                     TVRAHGQMASTPMTVIIDASIVDTAFPPPRAGLFAGAPLPGGPAPTYPTSGTRFPQGV
                     ASTLFQYGTATYDAYRLRFDSDVLHLAVETGANRWRADGAMQRILEGSGVEGAIALTI
                     DATTAVGTGNVYASTPIMLEYSRDRPETPLFFWSASTNGVMRGSLATSTASKLYPSSG
                     VCVGCHALSKDASRMAVGYDNTISTDLEQFDLATLAPTIPASTRQPMGWATYSPDGTH
                     LVVANNGALAMYDSTTGASLGAVPLPTMRYATHPDWSPDGRYLAVALTSQTPNNLDVK
                     AASIARIPYNDGAWGAPEVLVSGSSTNNNYFPKWSPDGRYLAFVHATSASQGAKSAEL
                     LLLPAEGGSPKYLATASHRVGGVDAGDLANTMPAWGPPQPEHQWLAFVSSRQYGDVLA
                     TGDRAQVWITSLDLDAPADSSSPAFWLPCQDVTVVNNNPVWTNQVVTN"
     gene            complement(8245..9345)
                     /locus_tag="VMZ53_22675"
     CDS             complement(8245..9345)
                     /locus_tag="VMZ53_22675"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HUS31341.1"
                     /translation="MKIRVEPPSPEVRRERVEKKLFAQLAAVEMAERVDAVLPQPRRS
                     RVPYMLGAAALAAAVLALVLFARRDAAPTHEVASPSRVVTPVGGESRFIVGDAVIDAK
                     SDTSVAVQHGEDGAITLVLDRGAVDCDVEPRHGRPPFRVLAGDLKVEVVGTRFTVTRT
                     PSLRVDVARGKVKVSTAAGTTLVEAGDSWPVVTAEAQPAPAVEQPVAEAPVAEAPVAE
                     APIADAPAPAPAPKATAPAVKKPAPVQPDEDLAQKSYRVAQKLETSQPAKAATIYRAI
                     ATDSTTTPALAATALLSLAQVELELHDPTAALSAAEEYVKRFPKAASAEEALWVRYQA
                     LRALGKRDEARGAAADYLRQFPNGAYADRLQK"
     gene            complement(9342..9935)
                     /locus_tag="VMZ53_22680"
     CDS             complement(9342..9935)
                     /locus_tag="VMZ53_22680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS31342.1"
                     /translation="MGLRLVDAPEPQDDTALIAACIAGDVAAQRTLFRREYARVNATV
                     YRIVGSPRDADDLVQETFIAVFRGLTRFRGDSKLSTWIDRIAVRVVFHHLRGKKSTVP
                     LDLIGELDAGGGRVDDQTHAREGLRRLYGVLADLSADSRMAFSLFAIDGRSIAEVAEL
                     TQTSLVAAKCRIWRARREVMKRAKADELLAELVKEAE"
     gene            complement(9966..>10180)
                     /locus_tag="VMZ53_22685"
     CDS             complement(9966..>10180)
                     /locus_tag="VMZ53_22685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31343.1"
                     /translation="AGNTVDSRRFDPAVRVGLAALRDLPRGLEVGLDVSADCLLLRQK
                     YEAGNEQILAVPRVQLVTSVVIGIRL"
BASE COUNT         1633 a         3302 c         3385 g         1860 t
ORIGIN      
        1 ccgaggcggc cttcggtacc gacttcgcgg gcgacgcggg tcacctcgtt cgcgaaaccg
       61 cggagctgct cgaccatcgt gttgatcgtg ttcttcagct cgaggatttc gcctttgacg
      121 tcgatggtga cggtcttcga caggtcgccc tgcgcgatcg ccgtcgtgac gcgtgcgacc
      181 tctcgcacct gcgacgtcag gttcgacgcc atcaggttca cggagtccgt caggtctttc
      241 cagacgccgc tgacgccttg gacacgcgcc tgtgcaccga gctttccttc gccaccgact
      301 tcgagagcga cgcgagtcac ttcggagccg aactgagaga gtcgagccac cattccgttg
      361 acgatgcggg catggcggag gaactcgccg cgccgcgggc cgaagccgcc gtcttcgaca
      421 tccatcgtct gctcgaggtc gcctttggcg acggcgctga cgacgcgcgc gatctcgttg
      481 gcgtgaccgg agagatcctc gatgacctcg ttgaccgact ggatgtaatc ggcccagccg
      541 cccgtggtgc cgagccggcg catgcgcttc gctgcctggc cctctttacc gacggcgctg
      601 cagacgtcgt gggactcttc cttgatctgc ttgaccatcg agacgagctc gttgaacgtc
      661 tcggcgacct ggccatcgag accggtcagg tcatcgcgca tgcgcacttc gaagttgccg
      721 cgcttgaacg cgcgcaaggc agcgagcagc tcgcgatggt tcaactgatc aggtgtgttc
      781 accttgaccg tcgtcttgcc attgccgttg ctgtggccgt tgccgttggc cttcttgtgg
      841 tgaccgttcc cgttaccgtt ggacttctcg ttccgggggg tatcgccgtg cgtgctcatt
      901 gttcctcttt gccgtagagc gctcgcatga tcttgtctgc gctcgccggt ttcacgatgt
      961 gggcatcgaa gcccgcttgc agagctgtcg ctcgatcggc tgcctggcca taaccagtca
     1021 tcgcgatcag gcgaaccggg tccgtcatca ggcggcgcag ctgacgcgcc acctcgtagc
     1081 catccatgtc gggaagcccg atatcgatca atgcgacgtc gggcttctcg aggctgatga
     1141 cacgaatggc cgagggtccg tcttcgacga cggtgacttc gtggcctttc atgcggagca
     1201 ggtcagcgac gagctcgcga agatccggtg catcgtcgca aacgacagcg cgcagccgcc
     1261 gctcgtgcgg ttccgtgtcg ggctttgtct gcgtgcgcga cgcctggagc gtgtcggcca
     1321 cgggcagctt cacttcgaag ctcgcacctt tgcccgaacc gtcgctattg gcggacaccg
     1381 tgccaccgtg gagctcgacg agtctcttca cgagaccgag accaagacca aggccgccct
     1441 tgccgtcggg cgtcgttcgc tcttggacga acatgtcgaa gatgcgcgga agcagctcgt
     1501 cggtgatgcc gcggccgttg tccttcacgc gaatgaacgc gagcttgtcc tcgccgatgc
     1561 cccagtcgac cacgatcgtg ccgcccgggt cggtgtactt caccgcgttc gacaagaggt
     1621 tcgtcagcac ctggacgagg cgcatcggat cgccatgaat gaccggcgat gcgtcgatac
     1681 cgtggacttc gagcacgtgc ttgcggcccg tgatcaccgg gcgcatgtcg ttgatcgctt
     1741 cggtgatgat cgcgtcgagc ggaacgtgct ctttgcgcag cgagagcttg ccgctctgga
     1801 agcgcgcgac gtcgagcaga tcgtcgacga gccggccgat gtgctggacc tgacgcgaga
     1861 tgatcccgcg aacgcgctcg ggcaccggtt tgtcggcggt gttcgccatg atctcgacgg
     1921 cggtctgcag cggctgcagc ggattgcgga gctcgtgggc gaggatcgcg aggaactcgt
     1981 ccttgcggcg atcgacttcg gccatctgtg cgacctgacg ctcgaggact tcggcttcga
     2041 agcgcttgcg ctgcatcgtc agctcgcgct cgtgcgcgcg ggtctgcgct tcacgcagct
     2101 gctcggcctt gtcgcgaagc tcgagcgtgc gtgcctgcag ctcgatgaag accatcgcct
     2161 tcgcacgcag cacctcgccg cggatcggct tcatgaggaa gtcgaatgcg ccgagctcgt
     2221 agcccttgag gatcgcgtcg tcctgccacg acaggccggt aatgaagatg atcggagtcg
     2281 cgcggttgcg agcgcgcgag cgaacgagac gcgccgtctc gaagccgtcc atgcccggca
     2341 tcgcgacatc gagaatgatg agcgcgaagt cctgctcgag caggcgtgcg agcgcttcga
     2401 caccagagcg tgcgagcacc atcttgcggc cgagcggtaa cagcgccgct tcgatcgcga
     2461 tcaggtttgt gtcgttgtca tcgacgacga ggatgtcgcg cccgtacgag agcagggcgc
     2521 catcttcctc gccctcgccg tcatcgagct ggtcggaatg caatggtgcg tctagctgca
     2581 cgaacccact tgtaaccgag gcagttgcca tatcgctctc cgtcatggcg cgttccgcac
     2641 gggcaccgtg gatcgatact cccggctgag taggcggaac ttcaccggtg gtgcgcatat
     2701 gctcaaccgg cggggcatgt gcacgcgatg tgcccgggtg acacgcgatc agagcgaccg
     2761 aaatttagac acttagctgt aaataacgcg cgcctgcgcg cactgcgcgc gcacgcacgc
     2821 atgggcaaaa atgctcactc agaacaggcg gggaccaggt cgtgtcccgc aaaaaactcg
     2881 tcgacaattc ccttccaagc gcggcggttt tcgggaggcc cgcacttgaa tgcagcgcgg
     2941 ctcaatttcc cgtttgcggc catctcggac tgctctttga agtagcgcac cgcgagctcc
     3001 gccgcgtatt cgtgcacggc gtctccgttg ttcggttgga acgcgtagta cgtgccgctc
     3061 gccttcactt tcgtgtcgtt cggcgcaaac ggagcgagac acttcaccgc cttcgcaccg
     3121 acggcgccgc acttcttgat gatcgcgtcg tagtcggtcg tcagcgtctt cgcggaccaa
     3181 tcgccgtgat cgaaatcgtt caggtgaaac agctcgtgga agtacgtctc gcggacgccg
     3241 cgttcgctcg tgagcagtga gccgaccacg ttatgcgtga tcgaccagcc ggtcgcgtac
     3301 gcgcttggcg tccgcttgcc gaccgaccgc acgaagtgaa cgtcgagctt gcgccagcga
     3361 tatttcggct tgtcgaagcc gacgaagaag tcgtcgatcg cgcgcagcgc tttgtcgacc
     3421 cacacgagct gctggcgata cttgccttcg ggcagctcgg gcacgagatg gatcttgccg
     3481 cggtaaccgc cgtccatgat ctcgtcgctg ccgacgtcga tcacatgctc gaggtcggcg
     3541 ttgagcgcgt ccgcgagcgc cttggctttc ttgtccttga agctcggacc cgcggccgcc
     3601 acgccacacg tcagaagaag gacgaccacc gaacgcatcc acttcatcct aatccgtaga
     3661 gacactcgat gctcgtcgaa gttgcggcgg cacgcccgat gctcgtcgag ggtgcatggt
     3721 gccgagaaac cgctttctgg ccgcgctcgc gtgcggcctg cctacgatcg tcgtcggtgt
     3781 ggcgagcgcg gatgactact atccgaccga cccgggcgtc gcgccgccac gctccgaatc
     3841 cctctggcgt gatccatccg tcgctagcgg cgtcggcatt ggcgtcgacg tcggcggcgg
     3901 tgtgacgggc ttcgtcggct cgcggctgcg cagcacgacg tcgtcgttcg gcggcgcgtg
     3961 gagcgttcgc gcggcgttcg gcacgcacat cccgctcgcg ctcgaggtcg gctacacggg
     4021 cagcgccacg gcgatcaacg gccagctcgg tccggagacg gccacgctga tgggacgcac
     4081 gctcgagtcc gcgattcgct acacggtgat gccgcgcgcg cggtggtcgc cgtacgcatt
     4141 cgctggtctc ggctggcagc actacacgat caacgatcgc agcttcgagc tgtcggacac
     4201 cggcattcgc acgagcgatg atttgctcgt gatgccgttc ggtctcggca tggggtatcg
     4261 cgtcggtgcg atcgtcacgg atctgcgagg cacgctgcgc gcggcgcgtg gcgcgaacct
     4321 cgtgctcgac aacgcggagg tcgcactcgc gacggggcag ggcagctacg cgccgatgca
     4381 ctcgtgggag acgagcctca acgtcggcta cgaattctag ggcttgtact cgtcggcgcc
     4441 gagatccttc ttcgcgcctt gtggccggaa gtcgccgtcg acgtcgttcg tgatcgtcga
     4501 cgtgatcgtc gaggcgtcga tcgccttgct gccgccgagc agatggtagt cgtacggcgg
     4561 tgtgatcgac ttgaactggt acggaactgc atcggcgtcc ggaggcgagc ccgtcgtgac
     4621 gcaattcgcc gcgttcccga gcgagcccgt attcgcgacg atcaagttgt tcgggacggc
     4681 aacgctgctc gcgagtgggc acgtgatgcc gccggcgagg atgagaccgt ccttgatctg
     4741 attgtgagcg acggtattga actcgaagcg attggcgggc gagctgctgc cgatgtcgac
     4801 gccgccatgc gccgatgtgg cagagccgtt gtggatgatg aagttgttcg tgatcgagaa
     4861 cgtgccgctc gcgctcgtga tgatgccgtc gaagttgcct gcaacggtcg actgatcgag
     4921 ctcgaggtag ctgttgtcct tgagctcgac gccgctcttc ggcgcgtcgt ggacgttgac
     4981 gcgatagagc cgagcggtgc tcgacgactc gacgcggagg cagttcatcg cgccgctaca
     5041 gtcgatgtcg aggccatacg cggtgacctg gctcgcattg atcttgacgg ccgggtcgcc
     5101 cttgccgagg agcttcgtgc cggggtcggt gacgatcacg acgtcgcgcg ccatgagcaa
     5161 gacggtttcg tcgagcgtgc ccgtgagctt gatgttcttg cgagtggcgg tcacgaggtc
     5221 gagcgcgtcg gcgatcgtct gtacgggctt cgtctttgtt ccgtcgccgt tcgctgccgc
     5281 cgcgggatcg acgtagatca cattcgcatc gtcgccacac acgccgccgg gaagacacgc
     5341 gctcgaaggg cagtcactgt gcgcgacaca gccgcggcac tcgagcgcgg tcgggtcgca
     5401 gttcagcttc gtcggatcgg tgcagtccgc gttcgagagg caaccgacgc acacgtgcga
     5461 gccgagctcg cagaacggcc tttgcgacgt cgtgcacgaa gcgttgtcgg tgcacatcat
     5521 caccggcacc tcgccgtccg tgcgcgggca gtgcgcgagg tcctcgggat tcttgtcgca
     5581 gtagagcgcg ctgcgcttct cgcacgcggc gagcagtgca acgagagcta gaacgcgcgc
     5641 gaccatccga tggtccctcc atgcgcatcg agtgtcgcaa ccggcgcgga atccgccggg
     5701 ccgccggcgc gaacccagag ccacgtgccg agcgcagtca cgacgacgcc gccggccgca
     5761 acgccgatgc cagcgagctt cgtgtcgcga tagtagagct tctcgccggt gtcctcttcg
     5821 ctcgtgacgt agagcaccgc gccacctgcg atcgcggcga cgccgaggcc gagcacgagc
     5881 gcgggcacca cgcgtgatgg cctgcttcga cggccttcgg gttcgtcgtc gacgggcatc
     5941 gtcaccggaa tcgtgatctc ggcgctgaca tcgggctgca ccttgagcga gcgctcgccg
     6001 acgcgttggc cgcgatgcat gagcacgatc tggtgcgtgc cgacggcgag gtcgcgctcc
     6061 atgggcgtca cgccgatgtt ctcgttgtcg atcaagacct gcatgccttc aggcttggag
     6121 tgcagcgcga cgcggccgcg cttctcggag ctcacctgcg tgacgaggcc gagcatctcg
     6181 tcgacgattc cgcgcatcac gtcggcggtg cagtcctcgc acgcgcggcg catgccgacc
     6241 ggctccttgt ctttgtagag ccaatagatc gtcaggatgg tcgctttgct cgtgcgcgac
     6301 ttcgcagctt ggccgtagag caccgcctcg gtcttcgcac gtgcggcgac gacgccctgc
     6361 gcgcacccct ggtcgtcgat ctccatgcag ttgagcagtg acctagtgcc gtcggcgccg
     6421 agcgcatcgg cgacttgatg gccgtgaccg cgcaaccagt tgtcgagctg cttggagagc
     6481 tccgcctgca gaccctgatc gccggtgacg acgatcccga cagtgccggc acgtgcgggc
     6541 gcggcgaaca gcatgaacag cgcgacgacg agcgccctca attcgtgacc acctggtttg
     6601 tccagacggg attgttattg acgaccgtca cgtcttggca gggtaaccaa aatgcggggc
     6661 tcgatgagtc agccggcgca tcgagatcga gagaggtgat ccagacctgc gcgcggtcgc
     6721 ccgttgcgag cacatcgccg tactggcgtg acgagacaaa cgcgagccat tgatgctcgg
     6781 gttgcggcgg gccccatgcg ggcatcgtgt ttgcgagatc gccggcgtcg acaccgccga
     6841 cgcgatggct cgcggtcgcg agatatttcg gcgagccgcc ctcggcgggc aggagcagga
     6901 gctccgcgct cttcgcgccc tgcgatgcag acgtcgcgtg cacgaacgcg aggtagcgac
     6961 catcaggtga ccacttcgga aagtagttgt tgttcgtcga cgagccgctg acgaggacct
     7021 cgggtgcgcc ccacgcgccg tcgttgtacg ggatgcgcgc gatgctcgcc gccttcacgt
     7081 cgaggttgtt cggcgtctgg ctcgtgagcg cgaccgcgag atagcggccg tccggtgacc
     7141 agtcgggatg cgtcgcatag cgcatcgtcg gtagcggcac ggcgcccagt gacgcaccgg
     7201 ttgtcgagtc gtacatcgcg agcgcgccgt tgttagcgac gacgaggtgc gtgccgtcgg
     7261 gtgagtacgt ggcccagccc atcggttgtc gcgtgctcgc cggaatcgtc ggtgcgagtg
     7321 tcgcgaggtc gaattgctcg aggtctgtcg agatcgtgtt gtcgtagccg acggccatgc
     7381 gactcgcgtc tttcgacagc gcgtggcagc cgacgcagac gccggagctc ggatagagct
     7441 tgctcgccgt cgaggtcgcg aggctgccgc gcatcacgcc gttggtcgac gcggaccaga
     7501 agaacagcgg cgtctcgggg cggtcgcgcg aatactcgag catgatcggt gtgctcgcgt
     7561 agacgttgcc ggtgccgacg gcggtcgtgg catcgatcgt gagcgcgatc gcgccttcca
     7621 caccgctgcc ctcgagaatg cgctgcatcg cgccgtctgc gcgccaccga ttcgcaccgg
     7681 tctccacagc gagatggagc acgtcggaat cgaagcgaag gcgatacgcg tcgtacgtcg
     7741 cggtgccgta ctgaaacagc gtgctcgcga cgccctgagg aaaccgcgtg ccgctcgtcg
     7801 gatacgtcgg tgccggacca ccaggaagcg gcgcgccggc gaacagacct gcgcgcggcg
     7861 gcgggaacgc ggtgtcgacg atggacgcgt cgatgatgac ggtcatcggc gtcgaggcca
     7921 tctggccgtg ggcgcgcacg gtgacgacgc cgtgacccgc ggcggtctgc gtcgcggtga
     7981 acagaccgtt gccatcgagc gcaccgattc gcacgttgtc gaccgaccag ccgacgagcg
     8041 acgtgacctc acgcgtggtg ccgtcggaga accggccgat cgcgatgtat tgcagcgtgt
     8101 gctcgggcgg cgccaggtcc gtcaccgtga tcacggcgtc cggcggatac acctcgagcg
     8161 attcgagacc gtcgagcgag tcggcctgaa cgcggatcgt ctcggtgcag cctgcaacga
     8221 gcgagagcag caggagtgcg cgaatcattt ctgcaaacgg tcggcgtatg ccccgtttgg
     8281 gaactgtcgc agataatccg ccgcagcgcc gcgcgcttcg tcacgcttgc caagcgcacg
     8341 caacgcttga tagcgaaccc acagcgcctc ttccgcgctg gccgctttcg ggaatcgctt
     8401 cacgtattcc tccgccgcgc tcagcgccgc ggttggatca tgcagctcga gctcgacctg
     8461 cgcgagcgag aggagtgcag tcgctgcgag cgccggcgtc gtcgtcgagt ccgtagcgat
     8521 cgcgcgatag atcgtcgcgg ccttcgccgg ctggctcgtc tcgagcttct gcgcgacgcg
     8581 atacgacttc tgcgcgaggt cttcgtcggg ctgaacaggc gccggcttct tcaccgcagg
     8641 ggccgtagct ttcggtgccg gggccggtgc gggcgcgtcg gcgatgggcg cttcggcgac
     8701 aggcgcttct gcgacaggcg cttctgcgac cggctgttcg acggccggcg cgggctgcgc
     8761 ttccgctgta acgaccggcc acgagtcgcc tgcttcgacg agcgtcgtgc ccgccgccgt
     8821 cgacactttc actttgccgc gtgcgacatc gacgcgaagc gacggcgtcc gcgtgacggt
     8881 aaaccgcgtc ccgacgacct cgaccttcag atcacccgcg agcacgcgga agggcgggcg
     8941 gccatgacgt ggctcgacat cgcaatcgac cgcaccgcga tcgaggacga gcgtgatcgc
     9001 gccgtcttct ccgtgttgca cggcaacgct cgtgtcgctc ttcgcatcga tcacggcatc
     9061 gccgacgatg aaccgcgact cgccgcccac aggcgtgacg acgcgcgacg gcgaggcgac
     9121 ctcgtgcgtt ggcgccgcgt cgcgacgagc gaacagcacg agtgcgagta ccgcagctgc
     9181 gagcgcagcg gcgccgagca tgtacgggac acgactgcga cgaggttgcg gcaacaccgc
     9241 atcgacgcgc tcggccatct cgacagccgc gagctgtgcg aacaacttct tctcgacgcg
     9301 ctcgcggcgg acttccggac taggcggctc gacgcggatc ttcattcggc ctccttcacc
     9361 agctcggcca agagctcgtc ggctttcgct cgcttcatga cttcgcgccg cgcgcgccag
     9421 atgcggcact tcgcagcgac gagcgacgtc tgcgtcagct ccgcaacttc ggcaatcgat
     9481 cggccgtcga tcgcaaacag cgaaaacgcc atccgcgagt ccgccgacag atccgccagc
     9541 acgccgtaga ggcggcgcag tccttcgcgc gcgtgcgtct gatcatcgac gcgaccgccg
     9601 cccgcatcca gctcgccgat cagatcgagc ggcacggtcg acttcttgcc gcgcaggtgg
     9661 tggaacacga cgcgcaccgc gatgcgatcg atccacgtgc tgagcttgct gtcgccgcga
     9721 aaccgggtga gcccgcggaa caccgcgatg aatgtctcct gcacgaggtc atcggcgtca
     9781 cgcggcgaac cgacgatgcg atacacggtc gcgttcacgc gcgcgtactc gcgccggaac
     9841 aacgtccgct gcgcggcgac gtcacccgcg atgcacgcgg cgatcagcgc cgtgtcatct
     9901 tgcggttcgg gtgcatcgac gagccgaagg cccatgcagc ctagtgtcgg cgaaagccga
     9961 ataagttaca gccggatgcc gatcacgacc gacgtcacga gctggacgcg cggaaccgcc
    10021 agaatctgct cgtttccggc ttcgtacttc tggcgcagca gtaggcagtc ggccgacaca
    10081 tcgagcccca cctcgaggcc gcgcggcagg tcacgaagcg ccgcgagccc cacgcgcacg
    10141 gccgggtcga agcggcgtga atccacggtg ttgccggcca
//
LOCUS       DATLBZ010000449         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6994497, whole genome shotgun sequence.
ACCESSION   DATLBZ010000449 DATLBZ010000000
VERSION     DATLBZ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6994497"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1082)
                     /locus_tag="VMZ53_22690"
     CDS             complement(279..1082)
                     /locus_tag="VMZ53_22690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31344.1"
                     /translation="MRSLFLLALTACGRLSFDGVDGHGDARRDGNGSDAPGDFCTAGA
                     SATCPAGALFCDGFETSAGPSFPKWDSTMLGNWFTGGAADPATQVDATGAPCRGAKSA
                     HGHALGEAQIATLHEAITPRPPSLFLRAWFRIGGTSPESDIEMLGIHDTPDSQFINVG
                     VMRGAQALGINVVGFASTAGGVESPATIVTDQWMCLELGIRFDTAGAGRIQLWLDDAP
                     IADLDNVTTEATATLDDVMLGVVTGMPETGTFDVDFDEVAVATSHIGCN"
     gene            complement(1092..1625)
                     /locus_tag="VMZ53_22695"
     CDS             complement(1092..1625)
                     /locus_tag="VMZ53_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31345.1"
                     /translation="MRMAWLVGLGLAMAACGGNDAAECTGTGCVCANTDCSCTAGADC
                     KTSCGDLDCSLACTSAAKCEGNTDGALMLACNDSSQCKGAGGDHSSVSCNAMSNCDFK
                     LGASAAVACNMSSTCKIQVGGASTIACSDSASCNIKCDTDCKATCTGSSECAVTCGAA
                     ATPATTCAPGVFACGSC"
     gene            complement(1640..>2321)
                     /locus_tag="VMZ53_22700"
     CDS             complement(1640..>2321)
                     /locus_tag="VMZ53_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31346.1"
                     /translation="PGEDPDQYIQRICGDKGPLARDCKTTVPEMQGHLVEAIAIRRAT
                     ERARNAVNECFACASDPGWHQAELGWETLDRTAAESVVEIETAADPGNWPTAGAAAED
                     DPGLPEAKLTTRGDLVVGGHSYGPNTQRVEVLRELRGNGDVIALHLHPDTTLAQARAI
                     LVDARKAGAKRVAVVAREAFYPYNRKVYWVADGYGMRANLRPTDSLQLLLHAIDEVAG
                     PGTVARVD"
BASE COUNT          367 a          791 c          778 g          385 t
ORIGIN      
        1 gacgcggtgc cggtcgggaa tccagtccgc cgcgatgccg acctcaccga cgacatcggg
       61 ccgcggcttg cccgtgatca ggccgccctc gagaccaccg cccagctgca cacccagcgt
      121 tgcgtcggca tgcgccacgc cggcagtgac cagcaacacg atgatggcga tgcgcacggc
      181 ccaagcgaac agcaatacgt acgccggcac cgtccgagtg gcatcgcggg cttatcgcgc
      241 gcgaggcgcg cggcagcgcg ccgtccgcga catcgctgtc agttgcagcc gatgtgcgaa
      301 gtggcgacgg cgacctcgtc gaagtcgacg tcgaacgtgc ccgtctcggg catgcccgtg
      361 acgacgccca gcatcacgtc atcgagcgtc gccgtcgctt cggttgtcac gttgtctaga
      421 tccgcgatgg gtgcatcgtc gagccagagt tggatgcgtc cggcgcctgc cgtatcgaag
      481 cgaatgccga gctcgaggca catccactga tcggtgacga tcgtcgcagg cgattcgacg
      541 ccgcccgcgg tcgacgcgaa gcccaccacg ttgatgccga gcgcctgcgc gccacgcatc
      601 acaccgacgt tgatgaactg cgagtcgggc gtgtcgtgaa tgccgagcat ctcgatgtcg
      661 ctttcgggcg acgtgccgcc gatgcgaaac cacgcgcgca agaacaacga gggcggccgc
      721 ggcgtgatcg cctcgtgcag cgttgcgatc tgcgcttcgc cgagcgcgtg accatgtgcc
      781 gacttcgcgc cgcggcacgg tgctcccgtc gcatcgacct gcgtcgccgg atcggccgca
      841 ccaccggtga accaattgcc gagcatcgtg ctgtcccact tcggaaagct cggccccgcg
      901 ctcgtctcga agccatcgca gaatagagcg cccgcaggac acgtcgcaga tgcgcctgcg
      961 gtgcagaaat cgcccggcgc gtcgctgccg ttgccgtcgc gcctagcatc gccgtggccg
     1021 tcgacgccgt cgaagctgag ccggccgcat gccgtcagcg cgagcaggaa cagactgcgc
     1081 acccagcgag actagcagga gccgcaggcg aacacgccgg gcgcacacgt tgtcgctggc
     1141 gtcgccgctg cgccgcacgt cacagcgcac tccgaagagc ccgtgcaggt tgccttgcag
     1201 tcggtatcgc acttgatgtt gcagctcgcc gagtcggaac aggcgatcgt gctcgcgccg
     1261 cccacttgga tcttgcaggt cgagctcatg ttgcaggcga ccgccgcaga ggcgccgagc
     1321 ttaaagtcgc agttcgacat cgcgttgcag gacacgctgc tgtgatcgcc accggcgcct
     1381 ttgcattgcg acgagtcgtt gcacgcgagc atgagggcgc cgtccgtatt gccttcgcac
     1441 ttcgctgcgc tcgtgcacgc gagcgagcaa tcgagatcgc cgcaggatgt cttgcagtcc
     1501 gcacctgcgg tgcaggagca atcggtgttc gcgcagacgc aaccggtgcc cgtgcattcg
     1561 gcagcgtcgt taccgccgca tgccgccatc gccaaaccga gacccaccag ccatgccatt
     1621 cgcatgaccg gatcgtagct caatcgacgc gtgcgacggt gccggggccc gcaacctcgt
     1681 cgatcgcgtg gaggagcagc tgcagcgagt cggtggggcg caggttcgcg cgcatgccgt
     1741 agccgtcggc gacccagtac accttgcggt tgtatggata gaacgcctcg cgtgcgacga
     1801 cagcgacgcg cttggcgccc gccttgcgag catcgacgag gatcgcgcga gcttgtgcga
     1861 gcgtcgtgtc gggatgcagg tgcagcgcga tcacgtcgcc attgccgcgg agctcgcgga
     1921 gcacttcgac gcgttgcgtg ttcggtccgt agctgtgacc accgacgacg aggtcgccgc
     1981 gcgtcgtgag ctttgcttcg ggtaggcctg ggtcgtcttc ggctgctgcg ccggcggtgg
     2041 gccagttgcc ggggtccgcc gcggtctcga tctcgacgac ggactcggcc gccgtgcgat
     2101 cgagcgtctc ccagccgagc tcggcttggt gccagcccgg atccgaagcg cacgcgaagc
     2161 attcgttgac cgcgttgcgt gcgcgttcgg tcgcgcggcg aatcgcgatc gcctcgacga
     2221 gatgaccctg catctcggga accgtcgtct tgcagtcgcg cgcgagcggg cctttgtcgc
     2281 cgcagatgcg ctggatgtac tggtccggat cttcgcccgg c
//
LOCUS       DATLBZ010000450        10038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6245066, whole genome shotgun sequence.
ACCESSION   DATLBZ010000450 DATLBZ010000000
VERSION     DATLBZ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6245066"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VMZ53_22705"
     CDS             complement(<1..584)
                     /locus_tag="VMZ53_22705"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUS31347.1"
                     /translation="MGSEASLKGSSARLWKLVEERMQQGANTAAIDQRIWDLFGEEWA
                     VVFTDLAGFSRHVAKFGIIHFLQVMLEQQRLLLPVVEQHDGILIKIEADSFLILFKKA
                     TQALHCAVAMQQKCQSVNMRRAPEEQIVLCCGIGFGKLLKIGDEDVFGHEVNVASKLG
                     EDTAKGNEILVTRAAKEAIGDSGVTWEEVHVEHAG"
     gene            616..1995
                     /locus_tag="VMZ53_22710"
     CDS             616..1995
                     /locus_tag="VMZ53_22710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HUS31348.1"
                     /translation="MKRLTPSDAMFLYGESREQMFHVAAMMPFTPPPDAPPDHLRQLM
                     DDIRAAGADVQRPWNLRLRTPDFLFNPMQAWVEAEEIDLEYHVRRSALPAPGDERELG
                     ILVSRLHGYHVDFHRPPWEAHLIEGLEGGRFALYVKVHHSLVDGFSAMRILANALSPD
                     PNERERPLFFSIPPPTRGPRPDAVGANYEAVMSAVREQYTAAKVATRALKRVFDSARA
                     GDHELVTPRQAPKCILNARISQSRRFATQRIEIGRLKAIAKAASGTLNDVVLTLCAGS
                     LRSYLLEQNALPAEPLIAMVPVAVRAKDDTGGGNSIGAILANLATDVEAADERLVQII
                     ESTRHAKQQLQGMSKAAIIQYSAMLIAPSMLQMIPQTAGHIRPTFNVVISNVPGPDHP
                     LYFRGARLEAAYPMSIPLHGQALNITANSYDGQMCFGFTGCRDTLPHLQRLAVYCREA
                     MDELERAFQ"
     gene            complement(1989..3644)
                     /locus_tag="VMZ53_22715"
     CDS             complement(1989..3644)
                     /locus_tag="VMZ53_22715"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HUS31349.1"
                     /translation="MIKDLWYKNAVFYCVHVGAFMDANGDGCGDFEGLMRRLDYLAGL
                     GITCIWLMPFQKSPQKDDGYDISDYYSVDERYGTLGDFVDFTHEAKQRGIRVIIDLVV
                     NHTSDQHKWFQEARKSKTSKYRDYYLWSEKRPKDADEGMVFPGVQKTTWTKDSVSGDY
                     YFHRFYDFQPDLNTQNPQVQEEIRKIMGFWLELGVSGFRMDAIPFVIQTKGADKKHTE
                     RYDMLRDMREFLQWRTGDAIILAEANVTPDHNLNYFGDLGDRIQMMFNFWVNQRVFYA
                     LASHDVRPLKKALQQTSNRPDTAQWAMFLRNHDELDLGRLTEEERELVFKEMGAEKTM
                     QLYDRGIRRRLAPMLNGDRRKLELAYSLMFSLPGTPIMRYGDEIGMGENLRLKERNAV
                     RTPMQWSTEPHAGFTRAEKPYLPVIDDGPYGFHKVNAAVQKTDPQSLLNWTERMIRAR
                     KEAPELGWGEFEVLETGQDKVLALRYNWRNNSVVTVHNVSGEALTIELHLGDMPGRKL
                     CNVLSPEHSEADGRGRHHIVLEPYGYRWYRVGGLGYLLDRTDY"
     gene            complement(3699..5021)
                     /locus_tag="VMZ53_22720"
     CDS             complement(3699..5021)
                     /locus_tag="VMZ53_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31350.1"
                     /translation="MELARGTVGEDRPWAITLATFARRGTSGQITLLADDGKRYSIAL
                     DRGMVVAARSPAIADTVGRIALTTRAASSDQINELVRRHGAQPHRDEIELLQELASLP
                     PSKVARLRWEVTARRAARSFCAERGEFILEDRICLPVGGCEVDLRAVVYQGLRLHASE
                     DRLRRELRALGEVFQLEPDAGTELHRFGFGDEEWPLLAALRTPITVPELEAVLRDLDS
                     RAMQAALSALAVFGTARAIATGRTPTPPRAHASSSMTPSIASTSLTPAPPIVPPARSP
                     LGRMSPAMGRAPTPLLNIPTSTRDLERISIGRTKTNPELAAEAAERATRALARDKPEA
                     AVLELKKAVELVPNDVDYSALLGWALFCAADDKRAVEPIARKALQLAVNKSTKPAVAR
                     FFLGRIERMLGRDREALGHFHAVLMLQPDHRDAAAEIRVLQARLEGRR"
     gene            complement(5140..5895)
                     /locus_tag="VMZ53_22725"
     CDS             complement(5140..5895)
                     /locus_tag="VMZ53_22725"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HUS31351.1"
                     /translation="MRALVRDVSPSFANALCAVTPDPPIELALAKAQHREYQLALLEC
                     GLELRMLGPSDDPDACFVEDTAVVASDVALISRPGAPSRRAETVTVAAALERDVQLVR
                     MDAPATLDGGDVMRVGKTIFVGRSARTNEAGIARLAEVFEPRGFRVVPVAMPAGVLHL
                     KSCCAPLGDDRITLADGSIPADAFGDVHVVRVPVDESYAANCLAVSGSVLCAKGYPRT
                     RDALAAAGFDTVELDTSEFRKADGALTCLSILF"
     gene            5930..7987
                     /locus_tag="VMZ53_22730"
     CDS             5930..7987
                     /locus_tag="VMZ53_22730"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUS31352.1"
                     /translation="MRSLVAFTLFAVACGGARPKQTVPAPPATGGDTTAAAQPPAPTP
                     EPAAQPPEPTPEMIGADTPKATVAGNTFVAPAGWSMIVRGTATVLTPPEGDSHIALVD
                     VNAKNADQAVKLAWIAYDPTKKYELKVATPAAPHDGWQEIQGYDYFVSPNEKRGVGAD
                     ARFANGQWLVTIYDMTNAVGEKRSSQVGVIFGKLLPKGYERETFAGKKANELTPERIK
                     ALGAFVEASEKLLGVPGVSIGVIQNGKTVFIGGFGVRELGKPAKVDGDTKYMVASNTK
                     AMTTLMLAKLVDEKKLTWDSTATSLLPSFKLGDADTTSKVMVKHLICACTGLPRQDME
                     WLLEYGKLTPEGALATLGTMQPTSKFGELYQYSNPLAAAAGFLGGHVAFPKLELGKAY
                     DQAMQARVFGPLGMKDTTFDFKKAEKGNSAVPHSPDVDGKTALALKEVNYSIIPVRPA
                     GAAWSTVRDMLKYVQMELAEGKLPNGKQYVSKDALLARRAPQVAIGKDATYGMGLMVD
                     TKYDVTVVHHGGDMIGFHSDMMWIPDAQVGAVILTNGDPGWLIRNQFRRKLLEVLYDG
                     KPEADANISAAAKQWFDGLAAERKLMTIPADSGESGRLAQSYSNDALGTVTVSKAGAA
                     TVFDFGEWKSEVASRKNPDGSISFLTTVPGMSGLELVVGNAGGKRTLVLRDGQHEYVF
                     TEK"
     gene            8018..8659
                     /locus_tag="VMZ53_22735"
     CDS             8018..8659
                     /locus_tag="VMZ53_22735"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS31353.1"
                     /translation="MPPSADVIRADFDRIARVSEAVPDRLEPAARRLLAEVPLRKQVL
                     DIGCGTGALARELAAKRGAKVTAIDLAPRMIDVARLRTSQSLRVDYRVADIMELTPRG
                     FDVAIAVNTLHHLPLAEIADRMASSVVSGGKVLIADLFTAPSVVDVPYNALSWLLAAR
                     SSSPELRDAWNAHGEHDELSPFRVIRATLREVLPGVVVRRHLGWRYTAVWQKK"
     gene            8741..9526
                     /locus_tag="VMZ53_22740"
     CDS             8741..9526
                     /locus_tag="VMZ53_22740"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS31354.1"
                     /translation="MGPCEGLIPDHLRSNVDTTEAAAWVVDGFGQAHAIGPKSTIGRN
                     HEGELVILASSVSREHAELVKKDAGWHIRDLGSRNGTFVDGTRAQGRVSLPERALLKI
                     GDVALWFLAEVVADPVPPPSMATASAGGGLVRFNIAAGPVELCLVGTTDMGAGGALLS
                     RAVGNEKWTEKNLPPLEFQLLRALCARAAEEADSPSAVRGCVQTKQLARDLPFQSKYA
                     NEENVRQVVRRVRAALGEIGADSMLAVAPGRGYYVSCPVTVAR"
     gene            complement(9523..9684)
                     /locus_tag="VMZ53_22745"
     CDS             complement(9523..9684)
                     /locus_tag="VMZ53_22745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31355.1"
                     /translation="MESAVLVVLAIVVALVAIDALGRLDERRPKSKPKKDQTVISTQK
                     IVVDLSKRS"
     gene            complement(9748..>10038)
                     /locus_tag="VMZ53_22750"
     CDS             complement(9748..>10038)
                     /locus_tag="VMZ53_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31356.1"
                     /translation="IQHVTAQPKRPSEVASTLIQPELETILMKCLEKKPADRYASATE
                     MAEALRALPPTKDWDLPDAKLWWREFKAREQAGLAASSLSTLTITVDLAKRE"
BASE COUNT         1730 a         3222 c         3310 g         1776 t
ORIGIN      
        1 ccggcgtgct cgacgtgcac ttcctcccac gtgacgccgg agtcgccgat cgcttctttc
       61 gcggcgcgcg tcacgaggat ctcgttgcct tttgcggtgt cctcgccgag cttgctcgcg
      121 acgttgacct cgtggccgaa cacgtcttcg tcgccgatct tgagcagctt gccaaagccg
      181 atgccgcagc agaggacgat ctgttcttcg ggtgcgcggc gcatgttgac cgactgacac
      241 ttctgctgca tcgcgaccgc gcagtgcagg gcttgggtcg ctttcttgaa gaggatcagg
      301 aagctgtcgg cttcgatctt gatcagaatg ccgtcgtgtt gttcgacgac ggggaggaga
      361 aggcgctgct gttcgagcat cacctgcagg aagtggatga ttccgaattt ggcgacgtgg
      421 cgggagaagc ccgcgagatc ggtgaacacg accgcccact cttcgccgaa gaggtcccag
      481 atgcgctggt cgatcgcggc ggtgttcgcg ccttgttgca tccgctcctc gaccagcttc
      541 cagagccgcg cagagctgcc ttttaggctc gcttcactcc ccataatccc cagcctacgc
      601 tgataggatc ggatcgtgaa gcgactcacg cccagcgacg ccatgttcct gtatggcgag
      661 tcgcgcgagc agatgtttca cgtcgcggcg atgatgccgt tcacgccgcc gccggacgcg
      721 ccgcccgatc atctgcgcca gctgatggat gacattcgcg cggcgggtgc cgatgtgcag
      781 cggccgtgga acctgcggct gaggacgcct gacttcttgt tcaacccgat gcaggcgtgg
      841 gtggaggccg aggagatcga cctcgagtat cacgtgaggc ggtcggcgct gccggcaccc
      901 ggcgacgagc gcgagctcgg catcctcgtg tcgcggctcc acggttatca cgtcgacttt
      961 catcggccgc cgtgggaagc gcacttgatc gaggggctgg agggcgggcg gtttgcgctg
     1021 tatgtgaagg tccaccactc gctcgtcgat gggttttcgg cgatgcggat cttggcgaac
     1081 gcgctgtcgc ccgatccgaa cgagcgcgag cggccgctgt tcttctcgat cccgccgccg
     1141 acacgcgggc cgcgacccga tgcagtaggc gcgaactacg aagcggtcat gagcgcggtg
     1201 cgcgagcagt acaccgcggc gaaggtcgcg acgcgagcgc tcaagcgcgt gttcgatagc
     1261 gcgcgggcag gggaccacga gcttgtcacg ccgaggcagg cgccgaaatg catcttgaat
     1321 gcgcgcatca gccagagccg gcgattcgcg acgcagcgga tcgagatcgg gcggctcaag
     1381 gcgatcgcga aggcggcgag cggcacgctg aacgatgtcg tcctgacgct gtgcgctgga
     1441 agcttgcgct cgtacttgct cgagcagaat gcgctgccgg ccgagccgtt gatcgcgatg
     1501 gtcccggtgg cggtgcgcgc gaaggacgac accggcggcg gtaactcgat cggtgccatc
     1561 ctcgcgaacc tcgcgaccga tgtcgaagcg gccgacgaac ggctcgttca gatcatcgag
     1621 tcgacgaggc acgccaagca gcagctgcag ggcatgtcga aggccgcgat catccagtac
     1681 agcgcgatgc tcattgcacc gtcgatgctg cagatgatcc cgcagacggc ggggcatatc
     1741 cggccgacgt tcaacgtcgt catctcgaac gtgcccggcc ctgaccatcc gctgtacttc
     1801 cgtggcgcgc gcctcgaagc tgcgtatccg atgtcgattc cgctgcacgg gcaggcgctc
     1861 aacatcacgg cgaacagcta cgacggtcag atgtgcttcg gctttactgg ttgtcgcgac
     1921 acgctgcccc acttgcaacg cctcgccgtg tactgccgcg aagcgatgga cgagctcgag
     1981 cgcgcgtttc agtagtcggt tctgtcgagc aagtagccga gaccaccgac gcggtaccaa
     2041 cgatagccgt acggctccag cacgatgtgg tgccggccgc gtccgtcggc ttcgctgtgc
     2101 tcgggactga gcacgttgca cagcttgcga cccggcatgt cgccgagatg gagctcgatc
     2161 gtgagcgctt cacccgacac gttgtgcacc gtcacgacgc tgttgttgcg ccagttgtag
     2221 cgcagcgcga gcaccttgtc ctggccggtc tcgagcacct cgaactcgcc ccagccgagc
     2281 tcgggcgctt ccttgcgcgc gcggatcatc cgctcggtcc agttgagcag cgactgcggg
     2341 tccgtcttct gcacggccgc gttcaccttg tgaaagccgt acggcccatc gtcgatcacc
     2401 ggcaggtatg gcttctcggc gcgcgtgaag cccgcgtgcg gctccgtcga ccattgcatc
     2461 ggcgtgcgaa ctgcgttgcg ctctttgagg cgcaggttct cgcccatccc gatctcgtcg
     2521 ccgtaccgca tgatcggcgt gccgggcagc gagaacatca ggctgtacgc cagctcgagc
     2581 ttgcggcgat cgccgttgag catcggcgcg agccgacggc gaatgccgcg atcgtagagc
     2641 tgcatcgtct tctcggcgcc catttctttg aagacgagct cgcgttcctc ctcggtgagc
     2701 cggccgagat cgagctcatc gtggttgcgc aggaacattg cccactgcgc cgtgtcgggg
     2761 cgattcgacg tctgctgcag cgccttcttg agcggccgca cgtcatgcga cgcgagtgcg
     2821 tagaacacgc gctggttgac ccagaagttg aacatcatct ggatgcggtc gccgaggtcg
     2881 ccgaagtagt tgaggttgtg atcgggcgtg acgttcgcct cggcgaggat gatcgcgtct
     2941 cccgtgcgcc actggaggaa ctcgcgcatg tcgcgcagca tgtcgtagcg ctcggtgtgc
     3001 ttcttgtcgg cgcctttggt ctggatgacg aacggaatcg cgtccatccg aaacccggac
     3061 actccgagct cgagccagaa gcccatgatc ttccggatct cttcttggac ctgtggattc
     3121 tgcgtgttga ggtcgggctg aaaatcgtag aagcgatgga agtagtagtc gccggagacg
     3181 ctgtccttcg tccacgtcgt cttctgcacg ccgggaaaca ccatgccctc gtcggcatcc
     3241 ttgggacgct tctccgacca caggtagtag tcgcggtact tcgacgtctt gctcttgcgc
     3301 gcttcctgga accacttgtg ttgatcggac gtgtgattga cgacgagatc gatgatcacg
     3361 cgaatgccgc gctgcttcgc ctcgtgcgtg aagtcgacga agtcgccgag cgtgccgtag
     3421 cgttcgtcga cggagtagta gtcgctaatg tcgtagccat cgtccttttg cggcgacttc
     3481 tggaacggca tgagccagat gcacgtgatg ccgagacccg cgaggtaatc gaggcgacgc
     3541 atgagtccct cgaagtcgcc gcacccatcg ccgttcgcat ccatgaacgc gccgacgtgg
     3601 acgcagtaga agaccgcgtt cttgtaccag aggtccttga tcacgcaccg acgctcgtcc
     3661 ggatggcacc ggcgatcaac tgtgcgtgag cgcccgcctt agcgccggcc ttcgaggcgt
     3721 gcctgcaaca cgcggatctc agcggcggcg tcgcggtgat cgggctgcag catgagaacg
     3781 gcgtgaaagt gaccgagcgc ttcgcgatcc ctgccgagca ttcgctcgat gcggccgagg
     3841 aagaaccgcg cgacggccgg cttcgtcgac ttgttcaccg cgagctgaag cgccttgcga
     3901 gcgatcggct cgactgcgcg tttgtcgtcg gcggcgcaga acagcgccca gccgagcagc
     3961 gcgctgtaat cgacatcgtt cggcacgagc tcgaccgcct tcttgagctc gagcaccgcg
     4021 gcctcgggct tgtcacgtgc gagagcacgc gtcgctcgtt cggcagcctc cgcggcgagc
     4081 tcggggttcg tcttcgtgcg gccgatcgaa atccgctcga ggtcgcgtgt cgacgtagga
     4141 atgttgagca aaggcgtcgg cgcgcggccc atcgcaggcg acatgcgtcc aagcggcgag
     4201 cgcgcaggcg gcacgatcgg cggcgctggc gtcagtgaag tcgaggcaat cgacggcgtc
     4261 atcgatgagc tcgcgtgcgc gcgcggtggc gtcggtgtgc gtccggtggc gatcgcccgc
     4321 gcggtgccga acaccgcgag tgccgacagc gctgcttgca tcgcgcgtga atcgaggtcg
     4381 cgcaacaccg cttcgagctc gggcacggtg atcggcgtgc gcaatgcggc gagcagcggc
     4441 cactcctcgt cgccaaagcc gaagcggtgc agctcggtgc ccgcatcggg ctccagctga
     4501 aacacctcgc cgagcgcgcg cagctcgcga cgcagccgat cctcgctcgc gtgcagccgc
     4561 aggccttgat agacgacggc gcggagatcg acttcgcaac cgccaaccgg caagcagatg
     4621 cgatcctcga ggatgaactc gccgcgctcg gcgcagaacg agcgagcagc acgacgcgcg
     4681 gtcacttccc agcgcaggcg cgcgactttc gacggcggca ggctcgcgag ctcttgcagc
     4741 agctcgatct cgtcacgatg cggctgcgcg ccgtgacgtc gcacgagctc gttgatctga
     4801 tccgacgacg ccgcgcgtgt cgtcagcgcg atccgaccga cggtgtccgc gatcgcaggc
     4861 gagcgtgcag cgacgaccat gccgcgatcg agcgcgatcg aatatcgctt gccgtcgtcg
     4921 gcgagtagcg tgatctggcc gctcgttccg cggcgcgcaa acgtcgccag cgtgattgcc
     4981 cagggtctgt cttccccgac ggtaccgcga gcaagctcca cactcccatc atcgggattc
     5041 cgtcgagacc atcaagattc atgattctcg tgagtcggct gacggaaggc tacccgacgt
     5101 gtacccgtcg ccgaggtgcg ccggtgtgcg tgcgcgacct cagaagagga tcgagagaca
     5161 ggtcagcgcg ccgtcggctt tgcgaaactc gctggtgtcg agctcgaccg tgtcgaagcc
     5221 tgccgccgcg agggcatcgc gagtacgcgg gtagcctttc gcacacagca cggagccgga
     5281 gactgcgagg cagttcgcgg catagctctc gtcgacgggc actcggacga cgtgcacgtc
     5341 tccgaaagca tcagccggaa tcgatccatc cgcgagcgtg atgcggtcgt cgccgagcgg
     5401 cgcacagcag ctcttgaggt gcagcacacc agctggcatc gcgaccggca ctacgcgaaa
     5461 gccgcgcggc tcgaagactt cagcgagacg tgcaatgccc gcttcgtttg tacgcgcgga
     5521 gcggccgacg aagatcgtct tgcctactcg catgacgtcg ccaccgtcga gtgttgcagg
     5581 cgcgtccatc cgcacgagct gcacatctcg ttcgagcgcg gcggcgacag tcaccgtttc
     5641 tgctcgccgg ctcggtgcac ccgggcgcga gatgagcgcg acgtcgctgg cgaccacggc
     5701 cgtgtcctcg acgaagcaag cgtcggggtc gtcggatggg ccgagcatcc tcagctcgag
     5761 accgcattcg agaagcgcga gctgatattc gcgatgctgc gccttggcga gggcgagctc
     5821 gatcggcggg tccggcgtca ccgcacaaag cgcattcgcg aagctcggcg agacatctcg
     5881 gaccagcgct ctcattgcac cgcgaccata ctgcgatatc gtccgcgcca tgcgcagcct
     5941 cgttgccttc acgctgttcg ccgtcgcctg tggcggagcc cgaccaaagc agaccgttcc
     6001 tgcaccaccc gcaaccggcg gcgatacgac cgcggctgct cagccgcccg caccgacgcc
     6061 cgagcccgcc gcccaaccgc cggagcccac gcccgagatg atcggcgcgg atacgccgaa
     6121 ggcgacggtt gccggtaata cgttcgtcgc gcctgcgggc tggtcgatga tcgtgcgcgg
     6181 aaccgcaacc gtgctgacgc cgcccgaagg cgactcgcat atcgcgctcg tcgacgtcaa
     6241 cgcgaagaac gcggaccaag ctgtgaagct cgcgtggatc gcgtacgacc caacgaagaa
     6301 gtacgagcta aaagtcgcga cgcccgcggc gccgcacgac ggatggcaag agattcaagg
     6361 ctacgactac ttcgtctcgc cgaacgagaa acgcggcgtc ggcgcagatg cacggttcgc
     6421 gaacgggcag tggctcgtca cgatctacga catgacaaac gccgtcggcg agaagcggtc
     6481 gtcacaggtt ggcgtcatct tcggcaagct gttgccgaag ggctacgagc gcgagacgtt
     6541 tgctggcaag aaggcgaacg agctcacgcc ggaacgcatc aaggcgcttg gcgcattcgt
     6601 cgaggcgtcg gagaagttgc tcggcgtacc gggcgtgtcg atcggtgtca ttcagaacgg
     6661 caagaccgtg ttcatcggcg gctttggcgt gcgcgagctc ggcaagcctg cgaaggtcga
     6721 tggcgacacg aagtacatgg tcgcgtcgaa cacgaaggcg atgacgacgc tgatgctcgc
     6781 gaagctcgtc gacgagaaaa agctgacgtg ggactcgacc gcgacgagcc ttctgccgtc
     6841 gttcaagctc ggcgacgccg acacgacgag caaagtgatg gtgaagcacc tcatctgcgc
     6901 gtgcaccggc ttgccgcgcc aggacatgga gtggctcctc gagtacggca agctgacgcc
     6961 ggaaggcgcg ctcgcgacgc tcggcacgat gcagccgacg agcaagttcg gcgagctgta
     7021 ccagtattcg aatccgctcg cggcagcagc gggcttcctc ggtgggcacg tcgcgttccc
     7081 gaagctcgag ctcggcaagg cgtacgacca agcgatgcag gcgcgcgtgt tcggtccgct
     7141 cggcatgaag gacacgacgt tcgacttcaa gaaggcggag aagggcaact cggcggtgcc
     7201 gcactcgccc gacgtcgatg gcaagactgc gctcgcgctc aaagaagtga actactcgat
     7261 cattcccgtg cgtcccgcgg gcgctgcgtg gagcacggtg cgcgacatgc tcaagtacgt
     7321 ccagatggag ctcgccgagg gcaagcttcc gaacggcaag cagtacgtct cgaaggacgc
     7381 tctgctcgcg cgccgcgctc cacaggtcgc gatcggcaag gacgcgacgt acggtatggg
     7441 cctcatggtc gatacgaagt acgacgtgac ggtcgtgcac catggcggcg acatgatcgg
     7501 gttccacagc gacatgatgt ggatccccga cgcgcaggtc ggcgccgtga tcctcacgaa
     7561 cggcgatccg ggttggctca tccgcaatca gttccgccgc aagctgctcg aggtgctcta
     7621 cgacggcaag cccgaagccg acgcgaacat ctccgccgcc gcgaagcagt ggttcgacgg
     7681 gctcgccgcc gagcgcaagc tcatgaccat cccggccgat tcgggagagt cgggcaggct
     7741 cgcgcagagt tattccaacg atgcgctcgg cacggtcacc gtcagcaagg ccggcgcggc
     7801 cacggtgttc gattttggcg agtggaagag cgaggttgcg tcgcgcaaga accccgacgg
     7861 cagcatctcg tttctgacca cggtgccggg gatgtcaggt ctcgagctcg tcgtcggcaa
     7921 cgccggcggc aagcgcacgc tcgtcctgcg cgacggtcag cacgaatacg tgttcacaga
     7981 gaaatagggt ggcagggcgc ggcgttgccc tgaacaaatg ccgcccagcg ccgacgtcat
     8041 ccgagccgat ttcgatcgga tcgcacgcgt cagtgaggcc gtccccgaca ggctcgagcc
     8101 cgccgcgcgc cgcctcctcg cggaggttcc gctgcgcaag caggtgctcg acatcggctg
     8161 cggcaccggt gctctcgcgc gcgagctggc ggccaaacgc ggcgcgaagg tcaccgcgat
     8221 cgatctcgcc cctcgcatga tcgacgttgc gcgcctgcgc acctcgcaga gcctgcgcgt
     8281 cgactatcgc gttgccgaca tcatggagct cacgccgcgc ggcttcgacg tcgcgatcgc
     8341 ggtgaacacg ctgcatcacc ttccgctcgc agagatcgcc gatcgcatgg cgagctcggt
     8401 cgtctctggc ggcaaggtcc tgatcgcgga cctattcacg gcgccgagcg tcgtcgatgt
     8461 tccctacaac gcgctgtcgt ggttgctcgc cgcgcggtcg tcgagcccag agcttcgcga
     8521 cgcctggaac gcgcatggcg agcatgatga gctctcaccc tttcgggtaa ttcgcgcgac
     8581 gttgcgcgag gttctcccag gcgtggtggt gcgtcgacac ctgggctggc gctacaccgc
     8641 ggtgtggcaa aaaaagtagt acgattgccc gcaaatgggc tgcttgtgct gcggtagcgg
     8701 ggcggaaatc ggatcccttt gccgaacgtg cgcccaagaa gtgggtccat gcgaagggct
     8761 cattccggac cacctccgct cgaacgtaga tacgaccgaa gctgcggcat gggtcgtcga
     8821 cgggtttggt caggcgcacg cgatcgggcc gaagtcgacg atcggtcgca atcacgaggg
     8881 cgagctcgtc atcctcgcga gctccgtctc gcgcgagcac gcagagctcg tgaagaaaga
     8941 tgccggctgg cacattcgcg atctcgggag ccgcaacggc acgtttgtcg atggcacccg
     9001 cgcgcagggc agggtgagcc tccccgagcg tgcactgctg aagatcggcg acgtcgcgct
     9061 gtggtttctc gccgaggtgg tcgcggatcc tgttccgcct ccatcgatgg cgacggcgag
     9121 cgcaggcggc ggccttgtcc gtttcaatat cgcggctggt cccgtcgagc tctgtctcgt
     9181 cggcacgacc gacatgggcg ccggcggtgc gttgctttcg cgtgcggttg gcaacgaaaa
     9241 gtggaccgag aagaatctgc cgccactcga gttccaactg ctgcgcgcgc tctgtgcgag
     9301 agcagcagaa gaagcagact ctccgtcagc ggttcgcggc tgcgtgcaga caaagcagct
     9361 cgcgcgcgac ttgccgtttc aatcgaagta cgccaacgaa gagaacgtgc gccaggtcgt
     9421 ccggcgtgta cgcgcagcgc tcggcgagat cggcgccgac tcgatgctcg cggtcgcacc
     9481 aggtcgcggc tactacgtga gctgcccggt taccgtcgcc cgctagcttc gcttcgacag
     9541 atcgaccacg atcttctgcg tcgagatcac ggtctggtct ttcttcggct tgctcttggg
     9601 gcggcgttca tccaatcgcc cgagcgcatc gatcgcgacg agcgcgacca cgatggcgag
     9661 gacaaccaag acagccgatt ccatgcccac ggtgtagcgc atcgtgccgc ttggtccgac
     9721 catttcacgc gtttttcacg ctcgtgatca ttcacgtttt gcgagatcca ccgtgatcgt
     9781 cagcgtggac agcgagctcg cggctaaccc cgcttgctcc cgggctttga actctcgcca
     9841 ccacagcttg gcgtcaggca aatcccaatc cttggtcggc gggagcgccc gtaaggcctc
     9901 ggccatctcg gtcgcgctcg cgtaccggtc ggccggcttc ttctctaagc acttcatcaa
     9961 gatggtctcg agctcgggct ggatcagcgt gctcgctacc tcggacggcc gtttgggctg
    10021 ggcggtcacg tgctggat
//
LOCUS       DATLBZ010000451         9680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4276926, whole genome shotgun sequence.
ACCESSION   DATLBZ010000451 DATLBZ010000000
VERSION     DATLBZ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4276926"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VMZ53_22755"
     CDS             complement(<1..750)
                     /locus_tag="VMZ53_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUS31357.1"
                     /translation="MSSSPQTSIRNVAIIAHVDHGKTTLVDGLLKQAGTFRTGEVVAE
                     RAMDSNDLERERGITILSKCTAVTWKGTRINIVDTPGHADFGGEVERVLGMVDSVLLL
                     VDAYEGAMPQTRFVTQKAFEMGLSPIVVVNKIDRPGVDPHDVMDQVFELFVSLGATDK
                     QLDFPVIYASGRQGYAIRELGDEPKDLGPILDLIVEKVPPAAGDPEAPLLMQVATLDY
                     DDYLGYMAIGRMRAGRAKVGDRVLLAHRDGKK"
     gene            complement(750..1334)
                     /locus_tag="VMZ53_22760"
     CDS             complement(750..1334)
                     /locus_tag="VMZ53_22760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31358.1"
                     /translation="MCSRLLCTLLVLAACQDKQPPAAPPAPASVDVTDADGHLVAELK
                     LIRPCRGSVPPFNELIVGGPPILASEGSAQWLGTTAANGTTYEKDGERLARLYPINDP
                     KTAAVLDMHGVAQVRILVEGDKGVVQNGAGMPIRNLSKGAAGSAGPSVVIDAPPLTVT
                     GTDDLVLAALISAPELVPQVRILAACERVLAKGS"
     gene            1423..2865
                     /locus_tag="VMZ53_22765"
     CDS             1423..2865
                     /locus_tag="VMZ53_22765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31359.1"
                     /translation="MKGCVALVGLASLLVVACGHRDSGNAGRRDAAAVPRDAAAPAEL
                     APLALGMASVEGFAYRTRPGHESFRRAREAEAKGDWAAVATACEAALATDPDHLDASY
                     LLAVARAKQNQLENIVPPLAKAVSSDFGKWAQASLDQPALQKFLATPAGEAWRARVDH
                     DRETFTASLARSLIVTSHGDFYAFDPDTSRWFRLTRTGGAVVGAFAPAGVARLAYITR
                     EKERLPSGAKGPTKIKLGIIDLATGRSRLAVELSGTGPLRVAYNAKKLKRFVVRVGAK
                     WFSVVDELKLRLEPEAKGAKIGYLADLADATWLDITGRTARLTRIGVPNVTADWDTQS
                     LASALRIGSSHRVVTVPSPGLIEGNTLVWSPDKTQLAFAAKLADTCKPGEPNAAAFVA
                     DGSTGAVQELERATAGIAVDWVAARKLAVAGDKGVTIVDLSGGAPLPLEGADGLLTPR
                     KKPTCTPEEPDVDIAEPIDEEPAEAETTQH"
     gene            complement(3160..3495)
                     /locus_tag="VMZ53_22770"
     CDS             complement(3160..3495)
                     /locus_tag="VMZ53_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31360.1"
                     /translation="MEPPADGQLRIRITPTLFIGPARFIIRPRISIDGVVEKTRWGTQ
                     IRTLPAGPHRLVVWFPWLLVPEAGRAELTVELVPGTVTSVRYHTTSFAIAGGRMTLDQ
                     SEIAGPATP"
     gene            complement(3590..3925)
                     /locus_tag="VMZ53_22775"
     CDS             complement(3590..3925)
                     /locus_tag="VMZ53_22775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31361.1"
                     /translation="MATPGQLSPEQLARLKRLLLDKGRDISDKLSQMMAGKKVTFEEM
                     FKAQVGETPIERARRYLDLVDGRIKAINAGTYGNCFLCETPLSYAELEQLPWAQYCTA
                     CAAKDTSNR"
     gene            complement(3904..5370)
                     /locus_tag="VMZ53_22780"
     CDS             complement(3904..5370)
                     /locus_tag="VMZ53_22780"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HUS31362.1"
                     /translation="MNFEPTTLGWLVPYLILCVAGMVLVLAEAFYKGEDRTYLSTLAV
                     AGSIAAAIASVILYRQLAPGETKPLMGTMLVADRLGYVLSAAFSLLTALTALMSPGHQ
                     RAHDWRMGEYYGLLLLSASGMVMLAQAANLVTIFLGIETMSIGVYVLVAMRRRSRRGN
                     EAALKYFLIGSFATGFLMYGLALLYGASGTLSLVRMQGALAGTANPGFVIAGAFMLVV
                     AFGFKVAAVPFHMWAPDAYEGAPTPVTAFMASAVKGAAVVAMIRVFGMALGGDVLPFG
                     TLGWASPLVVIAAITITIGNIGAVRQDNIKRMLAYSSISHAGVLMVGLCAMGLGSPTG
                     LSSLVYYILAYSVTTMGAFAVVAYVGSKGRERLLVDDWAGFASQHPAAALAMVVCLLS
                     LGGMPPTGGFFGKFYVFKSAMDVYNGQLLWLVLVGVVNSAISIYYYLRIVTAMYFREA
                     NAPFTVTRSSGLIFVMALMAILVLEMGIMPGWWLRLVS"
     gene            complement(5367..7043)
                     /locus_tag="VMZ53_22785"
     CDS             complement(5367..7043)
                     /locus_tag="VMZ53_22785"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HUS31363.1"
                     /translation="MTPGAYILPLLLGLPILGAIFVMCTPKTESSLHRGIGIAISTIT
                     FLVSLLIFKNFNPTADGFQLVFDTEWIPGLGAHFKTGIDGISVFLVILTTFLVPLTLI
                     GTRKAVDKHSREFMACILVLETGMLGAFVALDLFLFYVSWEVMLIPMYLLIGIWGGQR
                     RLYASIKFVIYTMVGSLLMLVAIFYVYVQGAAVLGHYTTDLQELLTVALPYKAQVWCF
                     AAFALAFAIKVPLFPLHTWLPDAHVEAPTAGSVILAGVLLKFGIYGFLRFAMPLFPHG
                     AAVWAPALAILAVIGIIYGAFVAFAQDDVKKLVAYSSVSHLGFCMIGILSITAMGTSG
                     SIYAMLSHGLTTGGLFLGIGVLYERRHTRRLSEYGGIWKQMPVFSGLFLIVVMGSAGL
                     PALSGFVGEFLTMFGTFMAYETEPMGYALPHPKLLAALSATGVILGAIYLLYMFQKVF
                     FGKLDKAKNGKLQDLRGHELFVFIVLALGIFIGGVYPKPILRLMEPSVQKFTRDFTRR
                     SAEPDGPPHVYGSLPTTPTPPPATNGAPPPAPPAGTPPAPAGAQPAGGTP"
     gene            complement(7040..9421)
                     /gene="nuoL"
                     /locus_tag="VMZ53_22790"
     CDS             complement(7040..9421)
                     /gene="nuoL"
                     /locus_tag="VMZ53_22790"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HUS31364.1"
                     /translation="MDTVNLKFFGIPLLACIPLLPLVGAFINLTLGRRFSKGMAHTVA
                     IAAVGLACVLSAYLVFGPLFKLFKAGQGGVGIEQTVYTWIEVGSFKAQLAFRLDTLSA
                     VMIMIITFIGSLIHIYSTGYMAHEGEQGYARYFGYLNLFTGSMLILVLAANLPVMFIG
                     WEGVGLCSFLLIGFWYENEAYATAGRKAFVVNRIGDFCFILGMFLLFWAVKDTSVANN
                     ARGSLDFANLKDPAVGASYVQAYWGGERLAAAAGILLFIGACGKSAQIPLFVWLPDAM
                     AGPTPVSALIHAATMVTAGVYMICRMSFLYSASTTALIVVSTVGLLTALAAAFMAFAQ
                     TDLKKVLAYSTVSQLGFMFVAAGTGNWVAAIFHLGTHAFFKACLFLGAGSVMHGMEHG
                     GSKTPGDIMTMGGLGKHMRITQITFAISCLAIAGIFPFSGFFSKDEILGGAWNVHPPG
                     WPVWYGKVLWGGLLLAALGTAFYMWRLYFLVFAGKERSEEAKHAHESPLSMTGVLAIL
                     AFFATIVGFLGLPHLESFHGPSITHGLAKWLEPSVTPTWYTPGTQEQMEILGHATDGT
                     TFALMGAALMIGLLGITLAYMFYGRGPSRSVDRLTEGPLADVYTASKHKLWFDEIYDV
                     TIVRPFKVVARGLFEIADRFVIDTVAVNGSAFVVGLFGRVSRWFQNGQVQRYLAGLVV
                     GAAAVFFVTDCHQNPSFAYRLNGNDLELHAEPGAGISSARAKMRWDIDGDGKPDNGPD
                     GKPLEAADIKVRAGEVGTHVTLWIDDPVTQKTVTVTRAIKFADAAPASSEVTP"
     gene            complement(9425..>9680)
                     /gene="nuoK"
                     /locus_tag="VMZ53_22795"
     CDS             complement(9425..>9680)
                     /gene="nuoK"
                     /locus_tag="VMZ53_22795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HUS31365.1"
                     /translation="GGVLVRRNALIILMSVELMLNAANMTLLTFSRMWGGTEALGAHT
                     FALMVIGVAAAEASVGLAIVVAVFRGRRNVDVDRLTTLRH"
BASE COUNT         1833 a         3123 c         3254 g         1470 t
ORIGIN      
        1 tttcttgccg tcgcgatggg caagcagcac gcggtcgccg acctttgcgc ggccggcgcg
       61 catgcgtccg atcgccatgt agccgaggta gtcgtcgtaa tcgagcgtgg cgacctgcat
      121 gagcagcggc gcctctggat caccggcggc gggcggcacc ttctcgacga tgagatcgag
      181 gatcgggccg aggtccttgg gctcgtcgcc gagctcgcgg atcgcgtagc cctggcggcc
      241 ggacgcgtag atgacaggga agtcgagctg cttgtccgtc gcgccgagcg agacgaacag
      301 ctcgaagacc tgatccatca cgtcgtgcgg gtcgacgccc ggacgatcga tcttgttgac
      361 gacgacgatg ggggacaggc ccatctcgaa tgccttctgc gtgacgaagc gcgtttgcgg
      421 catcgcacct tcgtaggcgt cgacgagcag gagcaccgaa tcgaccatgc cgagcacgcg
      481 ctcgacctcg ccaccgaagt cggcgtgtcc gggcgtatcg acgatgttga tgcgcgtgcc
      541 cttccacgtg acggcggtgc acttgctgag gatcgtgatg ccgcgctcgc gctcgaggtc
      601 gttggagtcc atcgcgcgtt cagcgacgac ctcgcccgtg cggaacgtgc cggcttgctt
      661 gaggagaccg tcgacgagcg tggtcttgcc atggtcgacg tgcgcgatga ttgcgacgtt
      721 gcggatactg gtctgagggg acgaagacat cacgatccct ttgcgagcac gcgttcacag
      781 gcggcgagaa ttcgaacttg cggcacgagc tccggtgcgc tgatcagcgc ggcgagcacg
      841 aggtcatccg taccggtgac agtaagaggc ggcgcgtcga tcacgacgga ggggccggcc
      901 gagcccgccg cgcccttgct gaggttgcga atcggcatgc cggcgccgtt ttgcacgacg
      961 cccttgtcgc cctcgacgag gatgcgcacc tgcgcgacgc catgcatgtc gaggactgcg
     1021 gcggtctttg gatcattgat gggatagagg cgcgcgagtc gctcgccatc tttctcgtac
     1081 gtcgtgccat tcgcggcggt cgtgccgagc cactgcgcgc tgccttccga cgccaggatc
     1141 ggagggccgc ccacgatcag ctcgttgaac ggcggtaccg atccgcggca cggccggatc
     1201 agcttgagct ccgcgacgag gtgcccgtcc gcatccgtga cgtcgaccga cgcaggtgca
     1261 ggcggcgccg caggcggctg cttgtcttgg caggctgcca ggaccagcag ggtacatagg
     1321 aggcgggagc acacgacggg cggcggttca taacagggga cggggagcac ggcaatgggc
     1381 taaaccttcg cgatccctgc aagtccgtga taaccccacg aggtgaaggg atgcgtcgcg
     1441 ctcgtcgggc tggccagcct gctggtcgtc gcatgcggcc atcgcgactc gggaaatgcc
     1501 gggcgccggg acgccgccgc cgtcccgagg gatgccgccg cgccggccga gctcgccccg
     1561 ctggcgctcg ggatggcctc ggtcgagggc ttcgcgtacc ggacgcggcc cggccacgag
     1621 tcgttccggc gcgcccgcga ggccgaagcg aaaggtgatt gggcggcggt cgccaccgcg
     1681 tgcgaggcgg ctctcgccac ggacccggat catctcgatg cgtcgtacct gcttgccgtc
     1741 gcccgcgcga agcagaacca gctcgagaac atcgtgccgc cgctcgcgaa ggccgtctcc
     1801 tcggacttcg gcaagtgggc gcaggcgtcg ctcgatcagc cggcgctgca aaaatttctc
     1861 gcgacgccgg cgggcgaagc atggcgcgca cgcgtcgacc acgatcgcga aacattcacg
     1921 gcgtcactcg cgcggtcgct catcgtcacg tcgcacggcg acttctacgc attcgatccg
     1981 gacacgtcgc gctggttccg cctcacgcgc acgggcggcg cggtcgtcgg cgcgttcgca
     2041 cccgcgggcg tcgctcgtct cgcatacatc acgcgcgaga aggaacgcct gccgagcggc
     2101 gcgaaaggcc cgacgaagat caagctcggc atcatcgacc tggcaactgg ccgctcgcgt
     2161 ctcgcggtcg agctctcggg caccggaccg ctccgcgttg cgtacaacgc aaaaaagctg
     2221 aagcgcttcg tggtccgcgt cggcgcgaag tggttcagcg tcgtcgacga gctcaagctg
     2281 cgtctcgagc ccgaagccaa gggcgcgaag atcggctacc tcgccgacct cgccgacgcg
     2341 acgtggctcg acatcaccgg ccgcaccgcg cggctcaccc gcatcggcgt gccaaacgtc
     2401 accgcggact gggacacgca atcgctcgcg agcgcgctcc gcatcggcag ctcgcatcgc
     2461 gtcgtgacgg tgccgagccc gggcctcatc gaaggcaaca cgcttgtctg gtctccggac
     2521 aaaacgcagc tcgcattcgc cgcgaagctc gccgacacgt gcaagcccgg tgagcccaac
     2581 gccgccgcct tcgtcgcgga cggctcgacg ggcgccgtgc aagagctcga gcgcgcgacg
     2641 gccggcatcg ccgtcgactg ggtggccgcg cgcaagctcg cagtcgcggg cgacaagggc
     2701 gtgacgatcg tcgatctctc cggcggcgcg ccgctgccgc tcgaaggtgc cgatggcctg
     2761 ctcacgccgc gcaagaagcc aacgtgcacg ccggaagaac cggacgtcga catcgcagag
     2821 ccgatcgacg aagagccggc cgaagcggaa acgacgcagc actgagagcc gccgaaccgg
     2881 aaacgcagcg ctgaggccga cgcatcggtc gccggacggc cgccagccgg acacgcgacg
     2941 aacgacgagt tctcccgcac gccccggaca ttcagtccga cctaatttcc cacgccagtg
     3001 ctccggaaat tcagtccgac ctaatttccc acgccagtgc tccgtaaccg tacgaggatt
     3061 gatcctgccg gttcgcgcag ctgcagctcg cggtgacgac gcgcagtcga cgcgacgtac
     3121 gagccacgct tgaagcagaa cgccgggtcg atggccagct caaggagtag ccgggccggc
     3181 gatctcgctc tggtcgaggg tcatgcgacc gcctgcgatg gcgaaggagg tcgtgtggta
     3241 acgcaccgac gttacggtgc ccgggaccaa ctccacggtg agctcggcac gcccggcctc
     3301 gggcacgagt agccacggga accagacgac gagccggtgc ggtcccgccg gcaacgttcg
     3361 aatctgcgtg ccccaccgag tcttctcgac cacgccgtcg atcgagatcc gagggcggat
     3421 aatgaaccgc gccgggccga tgaatagcgt cggtgtgatc cggatcctga gttgtccgtc
     3481 ggctggcggc tccacggcga cactgtacaa gatcagcgcg gtcgttactc gttggtctgc
     3541 gcggtcgccc tcgccggcgc tgctggaaat tcagtccgac ctaattcgtt tagcggttgc
     3601 tcgtgtcttt ggccgcgcag gctgtgcagt actgcgccca cggcagctgc tcgagctcgg
     3661 cgtaggagag cggcgtctcg cataggaagc agttgccgta cgtgcccgcg ttgatcgctt
     3721 tgatgcgacc gtcgacgagg tcgaggtagc ggcgcgcgcg ctcgatcggc gtctcgccga
     3781 cctgcgcctt gaacatctcc tcgaacgtca ccttcttgcc cgccatcatc tggctgagct
     3841 tgtcggagat gtcgcggccc ttgtcgagca gcagtcgctt gagtctcgcg agctgctcgg
     3901 ggctcagctg accaggcgta gccaccagcc cggcatgatg cccatctcga gaacgagaat
     3961 ggccatgagc gccatcacga agatgaggcc cgacgagcgc gtcacggtga acggcgcgtt
     4021 ggcttcgcgg aagtacatcg cggtcacgat tcgcaggtag tagtagatgc tgatcgcgct
     4081 gttgacgacg ccgacgagga ccagccagag gagctggccg ttgtagacgt ccatcgcgct
     4141 cttgaagacg tagaacttgc cgaagaagcc accggtgggc ggcatgccgc cgagcgagag
     4201 caggcagacg accatcgcaa gcgcggcggc cgggtgctgc gatgcaaagc cagcccagtc
     4261 gtcgacgagc aggcgctcgc ggcctttgga gccgacgtat gcgacgaccg cgaacgcgcc
     4321 catcgtcgtg acgctgtacg cgaggatgta atagacgagc gacgataggc ccgtcggcga
     4381 gccaaggccc atcgcacaga ggccgaccat gaggacgcct gcgtgcgaga tcgacgagta
     4441 cgcgagcatc cgcttgatgt tgtcttggcg gaccgcgccg atatttccga tcgtgatcgt
     4501 gatcgctgcg atcacgacga gcggcgaggc ccagccgagc gtgccgaacg gcagcacgtc
     4561 gccgccgagc gccatgccga acacgcgaat catcgcgacg acggctgcgc ctttgacggc
     4621 ggaagccatg aacgcggtga ccggcgtcgg cgcgccttcg tacgcgtcgg gagcccacat
     4681 gtggaacgga accgccgcga ccttgaagcc gaacgcgacg acgagcatga acgcgcccgc
     4741 gatcacgaag cctggattcg ccgtacccgc gagcgcgcct tgcatgcgca cgagcgagag
     4801 cgtgccggac gcgccgtaga gcagcgccaa gccgtacatg aggaagcccg tcgcgaacga
     4861 gccgatcaag aagtacttga gcgctgcttc gttaccgcgg cgcgagcgac ggcgcatcgc
     4921 gacgaggacg tagacgccga tcgacatcgt ctcgatgccg aggaagatcg tgacgaggtt
     4981 cgccgcctgc gcgagcatca ccatgcccga cgcgctgagc agcagcaggc cgtagtactc
     5041 gcccatgcgc cagtcgtgcg cgcgttggtg gcccggcgac atcagcgcgg tcagcgcggt
     5101 cagcagcgag aacgccgccg acagcacgta gccgaggcga tccgcgacga gcatcgtgcc
     5161 catcagcggc ttcgtctcgc cgggtgcgag ttggcgatag aggatgaccg atgcaatcgc
     5221 ggcagcgatc gagccggcga ccgcgagcgt cgacaggtac gtgcggtctt cgcctttgta
     5281 gaacgcctcg gcgagcacga gcaccatgcc cgcgacgcag aggatcagat acggaacgag
     5341 ccagccgagc gttgtgggtt cgaaattcat ggcgtacctc ccgcgggctg cgcgcctgca
     5401 ggagcgggtg gcgtaccggc cggcggagct ggcggcggcg caccattggt cgcgggcggt
     5461 ggcgtgggcg tcgtcggcag cgagccgtag acgtgcggcg gcccgtcggg ctccgcggag
     5521 cgacgcgtga agtcacgcgt gaacttctgc acgctcggct ccatcaggcg gaggatcggc
     5581 tttggataca caccgccgat gaagatgccg agcgcgagca cgatgaacac gaacagctcg
     5641 tgaccgcgca ggtcctgcag cttgccgttc ttcgctttgt cgagcttgcc gaagaacacc
     5701 ttctggaaca tgtagagcaa gtagatcgcg ccgaggatga cgccggtcgc cgacagcgcc
     5761 gcgagcagct tcgggtgcgg cagcgcgtag cccatcggct cggtctcgta cgccatgaac
     5821 gtgccgaaca tcgtcaagaa ctcgccgaca aagcccgaga gcgcgggcag acccgccgag
     5881 cccatgacga cgatgaggaa caggcccgag aagaccggca tctgcttcca aatgccgccg
     5941 tactccgaga gccggcgcgt gtggcgacgc tcgtagagca cgccgatgcc gaggaagagg
     6001 ccgcccgtcg tgaggccgtg cgagagcatc gcgtagatcg agccgctcgt gcccatcgcc
     6061 gtgatcgaca agattccgat catgcagaag ccgaggtggc tcacggatga atacgcgacg
     6121 agcttcttca cgtcgtcttg agcaaacgcg acgaacgcgc cgtagatgat gccgatcacc
     6181 gcgaggatcg cgagcgctgg tgcccagacg gcggcgccat gcgggaacag cggcatcgca
     6241 aagcgcaaga agccgtagat gccgaacttg agcagaacac cggccagaat gaccgagcct
     6301 gccgtcggcg cttcgacgtg cgcatcgggc aaccacgtgt gcagcgggaa cagcggcacc
     6361 ttgatcgcga acgcgagcgc gaacgcggcg aagcaccaga cttgcgcctt gtacggcagc
     6421 gcgaccgtga gcagctcctg gaggtccgtc gtgtagtgac cgaggaccgc cgcgccttgc
     6481 acgtagacgt agaagatcgc gacgagcatc agcagcgagc cgaccatcgt gtagatgacg
     6541 aacttgatcg acgcgtagag gcggcgctga ccaccccaga tgccgatcaa gaggtacatc
     6601 gggatcagca tcacttccca cgacacgtag aacaggaaga gatcgagcgc gacgaacgcg
     6661 ccgagcatgc ccgtctcgag gacgaggatg caggccatga actcgcgcga gtgcttgtcg
     6721 acggccttgc gcgtaccgat cagcgtgagc ggcacgagga acgtcgtcag gatcacgagg
     6781 aagaccgaga tgccgtcgat gccggtcttg aagtgcgcgc cgaggccggg aatccattcc
     6841 gtgtcgaaca cgagctggaa gccatccgcc gtcgggttga agttcttgaa gatgagcagc
     6901 gacaccagga acgtgatcgt ggagatcgcg atgccgatgc cgcggtggag cgacgactcc
     6961 gtcttcggcg tacacatcac gaagatggcg ccgaggatcg gaaggccgag gaggagcggc
     7021 aggatgtacg cgcccggggt catggcgtca cctcactgct ggctggtgcc gcgtcggcga
     7081 atttgatcgc gcgggtcacg gtcaccgtct tttgcgtcac tggatcgtcg atccagagcg
     7141 tgacgtgcgt gccgacttca ccggcgcgca ccttgatatc cgcggcctcg agcggtttgc
     7201 cgtcgggacc gttgtcgggc ttgccgtcac catcgatgtc ccaccgcatc ttcgcgcgcg
     7261 ccgaggagat gccggcaccg ggctccgcgt ggagctcgag gtcgttgcca ttcaagcgat
     7321 acgcgaacga cggattctga tggcagtccg tcacgaagaa tacggcggct gcaccgacga
     7381 cgaggccagc gagatagcgc tgcacttggc cgttctggaa ccaccgcgac acgcggccga
     7441 acaagccgac gacgaacgcg ctgccgttga cagccaccgt atcgatcacg aagcggtcgg
     7501 cgatctcgaa gaggccgcga gcgaccacct tgaacggacg cacgatggtc acgtcgtaga
     7561 tctcgtcgaa ccacagcttg tgcttcgacg cggtgtagac gtcagcgagc ggaccttccg
     7621 tcagccggtc gacagagcgc gacgggccgc gcccgtagaa catgtacgcg agcgtgatgc
     7681 cgagcagacc gatcatgagc gcggcgccca tcagcgcgaa cgtcgtgccg tcggtcgcat
     7741 gcccgaggat ctccatctgc tcctgcgttc cgggcgtgta ccaagtcggc gtcacgctcg
     7801 gctcgagcca cttcgcgaga ccatgcgtga tcgacggacc gtggaagctc tcgaggtgcg
     7861 gcagcccgag gaagccgacg atcgtcgcga agaacgcgag gatcgcgagc acacccgtca
     7921 tcgacagcgg cgactcgtgc gcatgcttgg cctcttcgct gcgctccttg cccgcgaaca
     7981 cgaggaagta gagccgccac atatagaacg cggtgccgag cgccgcgagg agcaggccgc
     8041 cccagaggac cttgccgtac caaaccggcc agcccggcgg atggacgttc catgcgccgc
     8101 cgaggatttc gtctttcgag aagaatcccg agaacgggaa gatgccggca atcgcgaggc
     8161 acgagatagc aaacgttatc tgcgtgatgc gcatgtgctt gccgaggccg cccatcgtca
     8221 tgatgtcgcc gggcgtcttg ctgccgccgt gctccatgcc gtgcatgacc gagcccgcgc
     8281 cgaggaacag acacgctttg aagaacgcgt gcgtgccgag gtggaagatc gcggcgaccc
     8341 agttgccggt gcccgcggcg acgaacatga agccgagctg actcaccgtc gagtaggcga
     8401 gcaccttctt caagtctgtc tgcgcgaacg ccatgaatgc agcggcgagc gccgtgagta
     8461 agccgacggt cgatacgacg atcagtgccg tcgtcgatgc cgagtagagg aacgacatcc
     8521 ggcagatcat gtagacgccg gcggtgacca tcgtcgccgc gtggatgagc gcggagaccg
     8581 gcgttggacc tgccatcgcg tccggaagcc agacaaacaa cggaatctgc gcgctcttgc
     8641 cgcacgcacc gatgaacaac aggattcccg ccgctgcggc gagccgctca ccgccccaat
     8701 acgcttggac gtacgacgcg cccaccgctg gatccttgag gttcgcgaag tcgaggctgc
     8761 cgcgcgcgtt gttcgcgaca gacgtgtcct tcacggccca gaacaacagg aacatgccga
     8821 ggatgaagca gaagtcgccg atgcggttga cgacgaatgc tttgcgacct gccgtcgcgt
     8881 acgcctcgtt ctcgtaccaa aagccgatca gcaggaacga gcacaggccg acgccttccc
     8941 agccgatgaa catcaccggg aggttcgcgg cgagcacgag gatcagcatc gagcccgtga
     9001 agaggttcag atagccgaag taccgcgcgt agccctgctc gccctcgtga gccatgtagc
     9061 ccgtcgagta gatgtgaatc agcgagccga tgaacgtgat gatcatgatc atcacggccg
     9121 acagcgtatc gaggcggaac gcgagctgcg ccttgaagct gccaacctcg atccacgtgt
     9181 agaccgtctg ctcgatgccg acgccgccct gccctgcttt gaacagcttg aacagcgggc
     9241 cgaacaccag gtacgcggag agcacgcacg cgagaccgac cgcggcgatc gcaacggtgt
     9301 gcgccatgcc cttcgagaac cggcggccga gcgtcaggtt gatgaacgcg ccgacgagcg
     9361 gcaacagcgg gatgcacgcg aggagcggaa tcccgaaaaa cttgagattg accgtatcca
     9421 tcgcctagtg cctcagggtg gtgaggcggt cgacatcgac attgcgccgg ccgcggaaca
     9481 ccgcgacgac gatcgcgagg ccgaccgagg cctcggctgc agcaacaccg atcaccatca
     9541 gcgcgaacgt gtgagcaccg agtgcttcgg taccgcccca catgcgcgag aaggtgagca
     9601 gcgtcatgtt cgccgcgttg agcatcagct cgacgctcat caagatgatc agcgcgttgc
     9661 ggcgaacgag cacgccaccg
//
LOCUS       DATLBZ010000452        19114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1318891, whole genome shotgun sequence.
ACCESSION   DATLBZ010000452 DATLBZ010000000
VERSION     DATLBZ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19114
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318891"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..2278)
                     /locus_tag="VMZ53_22800"
     CDS             complement(188..2278)
                     /locus_tag="VMZ53_22800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HUS31366.1"
                     /translation="MAGEPELTLDGTLERITFRGDEYYTVARFAPDSGAAITIVGKLV
                     EINEGMPITIHGQWVEDKKYGRQFKVSFAHPRTPKTVIGIERFLASGLIKGVGPAIAK
                     RIVEKFGKDTLEVIDKSPHRLTEIAGIGTAKAKQVADAIAAQKHVQDVLVFLQGHGIT
                     MASAARIVRQYGKDAINVVRANPYRLAHEVWGIGFRTADAIAEKLGIARDAPERLEAG
                     LLHALETSAEDGHMHLPDEELVAAAAALLGIDAAKLPVRLGALEHSKKVVREVLGHRG
                     PCTALPDLYAAEHESAALLAEIAATPARSFPLDIAAAVHAFESVTGLELARQQRQAVE
                     ASLRDRCVVITGGPGVGKTTIVKAIVHLARLGSRRVSLAAPTGRAAKRLGEATAAEAM
                     TIHRLLEFNPNEGGFQRSRENPLDADMLVVDEASMVDALLFRAVLGALRPGAQLVLVG
                     DVDQLPSVGAGAILADVIESGAATVIRLTEIFRQAAASKIVVSAHRINRGELPDLDSA
                     PGGDSDFYFITRDDPNAARDTIVELVAERIPARFKLDSISDVQVLTPMHRGDLGTASI
                     NRALQDRLNTGTPGSQELVRGDRTFRPRDKVMQLKNDYDRNVFNGDIGVIEAIDHDAG
                     TLRVDFDGRIVPYERAELDQLVHAYAVSVHKSQGSEYPAVVIPLVTQHFMMLGRSLLY
                     TAVTRGQKLVVIVG"
     gene            2317..3192
                     /locus_tag="VMZ53_22805"
     CDS             2317..3192
                     /locus_tag="VMZ53_22805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31367.1"
                     /translation="MRRSLVVLAFVGACGGIDLGEYPDEAVDARCEYLVNCGLFEDAN
                     ACRAYYQDRLPNNPSVEAAVDDGKLAYDGDAADDCLDALRSGSCSRGAELPDTCDHIF
                     TGKVADGGACAFDAECVSDFCDVSDCTMACCPGMCSPSRPVPKIGQQCNFICEDGAYC
                     GLDNVCKAVLPKGASCDYPLACDRGLYCAGMTGSTAGICTQMPKTGEACTEICDDIGD
                     TCKAGTCTPVGLSGDPCTTDDDCSVYYECDGTKCVVPASDPKMPNGSQCSSSVQCQSH
                     YCGNDNLCADVPLCI"
     gene            complement(3189..3473)
                     /locus_tag="VMZ53_22810"
     CDS             complement(3189..3473)
                     /locus_tag="VMZ53_22810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31368.1"
                     /translation="MKSGLYGAIIALLLTGCIVRHLVSGPRLTGTCNGACAHYVDCKP
                     GARAEDRTRCETECPDVFSDRDSLMAYESLSCQNAVEYVDGTQPKAARKH"
     gene            complement(3470..4225)
                     /locus_tag="VMZ53_22815"
     CDS             complement(3470..4225)
                     /locus_tag="VMZ53_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HUS31369.1"
                     /translation="MVRVERSGRVTTVILDRAEAKNAVDRPTAEALADAFREFDADPE
                     ARVGVLYGDHGTFCAGADLKALAKGTPNRVAPDGDGPMGPSRMLLGKPVIGAIEGHAV
                     AGGLELALWCDLRVAADDAVLGVFCRRFGVPLIDGGTIRLPRLIGLSRALDLILTGRG
                     VDAQEALAMGLVNRVVPKGEARAAAERLAMELAELPQAAMRADRMSAYLQHDLSLEQA
                     LQKELELGSVALQEAVEGAMRFAGGAGRHGSRS"
     gene            4249..5358
                     /locus_tag="VMZ53_22820"
     CDS             4249..5358
                     /locus_tag="VMZ53_22820"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HUS31370.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAATLIGRAGGADILGVERGYRGL
                     LDDAFVELGPQDVAGILREGGTMLGSARCKEFHDRKVRDRAREILAKREVDGLIVVGG
                     NGSLTGALHLADPAEIGSGRPLKVTGVPASIDNDLGLTGLSIGVDTAMNTIVEACDKI
                     ADTATAHDRTFIVEVMGRDCGYLAMTSAIAVGADLALFPEANKPENETIQQIVDTVLA
                     VRAKQKRARKVLIIKAEGVNISADKLKLAVDAGLKERQPDAEPSSIETRVTVLGHVVR
                     GGRPSAFDRLLGSRLANVAVRALLAGESHKMAAWMPPVELPAEVGSRSPADPYCWLVD
                     LKAVLAETENLLKGRSPLARWRAGAFEEIEDALLL"
     gene            5369..7207
                     /locus_tag="VMZ53_22825"
     CDS             5369..7207
                     /locus_tag="VMZ53_22825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31371.1"
                     /translation="MPTEPELLRLFDRSPIGMYRSDARGRLLVVNPALVAILGYDTVE
                     EVLALDLLKDVYINAAERAPVLEEYRDTGFVEGRRVHWKARTGRILTVQIFGHVVETK
                     DGLVFDATVIDLTEIDALEDELRRQREILEQSMRQMPAVYWVTDREQRLTHVGGPTEQ
                     LFGFPPERFLGKKIQDALVDPKDDIDAVRNHDRALAGEIIQHESLYAAKLVASTTAPY
                     RNANGDIIGVIGTLVDVTAWRALERRMVDAQRAESLGVLAGGLAHDFNNLLVAVLGNA
                     DLALREIPVGKPGRTAIENIRVAGLRAAELTDQLLAYAGRGGAGTTRVELASIAEELL
                     RIAAPQFPPEVRVALDIETKLAVRGDPAQVRQVLMNLISNAREALLGRGGKIAVTAET
                     VHSAGDADLDDMLTAPKGSYVAIRVVDNGPGMDRETRRHIFEPFFTTKQGGHGLGLAA
                     VLGIVRAHGGGVRVRSEPDAGVEITVLWPAAVTAPIAAAQPPTTAPTVLVIDDEALVR
                     DVVARMVEDLGYLAMTAADGQTGLALLDKETIDAVLVDLTMPQMSGADVVAAVRTKRP
                     GLPVVICSGYDRDGRGPVQADAYLPKPFRLDALERTLAKLLPLRSV"
     gene            7247..7957
                     /locus_tag="VMZ53_22830"
     CDS             7247..7957
                     /locus_tag="VMZ53_22830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS31372.1"
                     /translation="MSEAAASTIKVLLVEDDARLAQLTIRYLEGHGVVVTHASDGIEG
                     QAEALRRQYDCLVLDLMLPGRDGIEVCRQLRSRIDVPIIMVTARGEESDRVLGLEVGA
                     DDYVTKPFSPRELLARIKANVRRQRGQAGPVAQTIQVGKLVLDPAAMTVVLDGNPVDV
                     TAYEFSILRALAQRPGKVLSREQLLDLAKGSADLSFDRSIDVHISRLRNKLGDDSRSP
                     KILKTVRGVGYLLAGGTE"
     gene            7954..9258
                     /locus_tag="VMZ53_22835"
     CDS             7954..9258
                     /locus_tag="VMZ53_22835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS31373.1"
                     /translation="MSKRKLALRLYALTAAIAIVTMVALLLLPRFVGSPRYLEPQAAL
                     VQNLVDRFSLREPERRAEPLERWRKRLKGKLTFYDTQGSVIASTHEELPPPTTDELKG
                     LEGDQKWILEYRRIVVRSDDGKMIGVYVPNTPGFPWGFVLPLGAGVLIVVGAATLWFS
                     RRLVRPLDSLAMAAQKFGSGDISSRAHLHRDDELGDVGKAFDDMADRTAALISSQRQL
                     MADVSHELRTPLARIRVALELAAEDPLAAKDVLTDVGTDLDEIDQLIADILTTARLDA
                     DPAPVAREPISVGELADRAASRFTARHPGRTLEKTVDDAERAIECDPVLLRRALDNLL
                     DNAAKYSEADKPITLAVHPNGKAVDFEVVDKGIGMSKDELARAGTPFWRADGSRARRT
                     GGVGLGLALARRIARAHGGDISLDSVPGRGTTVRLHVPVGES"
     gene            9255..10622
                     /locus_tag="VMZ53_22840"
     CDS             9255..10622
                     /locus_tag="VMZ53_22840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUS31374.1"
                     /translation="MIKRWLPRIIVLAIVAGGVAFLWIKKPWQKAPTPVTFSTVPVTK
                     GTIAAQVTATGTLSARGTVQVGAQVSGRVVELKADFNDKVKKGQIIAKLDESVLKAQI
                     QQQQAAYDLTVANLKKSQVSAMDAKRQLERQRTLKDQNLIAGATVEQYEVTLETANAQ
                     IVASKAEVARAAANLEQARTNLGYATIISPTDGVVLSRAVELGQTVASSLQAPVLFTI
                     AEDLTRMQIDTAVAEGDVGALREGMKAQFSVDAFPGKTFEGVVRQVRNAPTTTQGVVT
                     YDAVIDVDNTDLSLRPGMTASVTFVLQSVENVVKIPNAALRFKLSRDQMMALAEKYNL
                     PMGRRGSGAGGGSGSWSGRRGSGAGSGGGELAQGSGASGGGGGPGGGGGGPGGGGEGG
                     GRRMGDRKQLWKLVDGKPKMVFVKTGLTDGSTTQLLDGDIQPGDQLITEIQGLPAPKR
                     KMGAF"
     gene            10622..11329
                     /locus_tag="VMZ53_22845"
     CDS             10622..11329
                     /locus_tag="VMZ53_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS31375.1"
                     /translation="MAAPLVEMRGISRTYQMGDNLVHALRNVSITIERGASIAIMGTS
                     GSGKSTLMNIMGCLDQPTSGEYLLSGGDVSKLSRSQLADVRGKMIGFVFQSFNLLTRT
                     TALENVETPLIYQGVSARERRKRAIEALERVGLHGRHDHTPNQLSGGQQQRVAIARAL
                     VTKAPVIMADEPTGNLDTKTSYEVMAILQGLVDQGITVVLVTHEADIAAYARRVVTLR
                     DGAIVEDKTQEPVKAVA"
     gene            11326..12555
                     /locus_tag="VMZ53_22850"
     CDS             11326..12555
                     /locus_tag="VMZ53_22850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS31376.1"
                     /translation="MNIFTTFLLALRALWRHKVRTFLTMLGLIGGVGTVIAMVAAGQG
                     ARESVADVFRSMGTNLLIISNGSQRQGGVAGGTGSSFALTWDDFAALENGEVSTVRWV
                     SPVLATKMQVAAEDTNWNTQVTGTNAAFFKIKNWDVTEGKLFDDDVGPAKIALIGKTV
                     ATTLYPGVNPIGQAIRINGQPYEVTGILASKGQSAMGQDQDDIVVVPVKTYLAKLDRG
                     AGKFITRGQIYVSTESENDITRAEQQISELLRQRHNLGPNDEDDFRIRNLAEFALRQK
                     ESTDKITTLLALVAAMALLVGGIGVMNIMLVSVIERTREIGIRMAVGAKPLDVMTQFL
                     VESLMLSLIGGAIGLAMGAWWAKLMADFYGWKFLFPAQTAVIAFLISSGIGVVFGLYP
                     AIRASLLDPITALRYET"
     gene            12552..13850
                     /locus_tag="VMZ53_22855"
     CDS             12552..13850
                     /locus_tag="VMZ53_22855"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUS31377.1"
                     /translation="MKLRIALCLALLSSPALAQPPPQTLTMEQAVEIAMKSQPALRQS
                     RASLEASRGRIEQVEATRRPTLNLSASANIGGGGPSRDIIDPSASSAIGASAQWRITD
                     FGQTAAQLRAAQLSAEASSADVQTDILDVRRDVELTFLEAVARTRLLVVAEATVKSEE
                     THLDQAKRFVAAQAKDPIEVVQAQARAANARASFAQAQSQAAIALANLRAAIGWVDPA
                     STLAVSNNWPVPPDAPPPVLTELVTTARSHRPEIVALDKQVAAAEANIDAARAQRRPV
                     LSANAQSQWSPSWSKEADMNGDHINVPQPSWSIGLSLTWQAYDGGRTAADVRVARANR
                     ESALAQRDALLLDLTSQLDSARAQIDANRAATQASTEAVEAARAQLELAEARYAQGLG
                     SQIELADAQTAVTTAEGNLIQSEFQLATAWATLRRSLGGI"
     gene            13887..16553
                     /locus_tag="VMZ53_22860"
     CDS             13887..16553
                     /locus_tag="VMZ53_22860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31378.1"
                     /translation="MGIRAFWPSAFALGITLLAACGDNLEPPDDPDQLVNVELETGAP
                     SQVMAGDTINVTCTLHERDMNGEDLATMVPAEIKIVDETKVSRLSGTIIARKVGIIAV
                     SCQLPDRGVADASPVMVEILPGAASNVVTTVTPNPVVAGNDIAVTCTVYDDYGNLVEG
                     QSPTLALAPDDNANTVAGLGATMIHSGHYIATCQLPGSTSNNAGFDVLPNLPANISLS
                     RAPDLPVYAINDTIQILHVITDRYGNEIFDATVTDVSTPLVGTGPTTYLGASTFKYGG
                     EGTFRITVTVDPPTDGGVTVSASINVLINSRGPAISCLNDATMLNLTPGGTYTVNGNA
                     NDVNGVKSLTVNGNAVTFNGNGDWTANITTRFGMNFVDITAIDNFDEPTTKVCTFLVS
                     NRYAATTTPIPDVLSLKLTQPAVDDFNRTNTLGSFGDILHTIANSQGLRDAVHNALYA
                     NNPLKPSSCDSQTCVPFIGCACWYSSGVWYNYSQFDGPNTVSLTLVDGGIAATARLEN
                     VHVNLRVWGKVGPIPYDTTGWVNVSYVQVNLTLDTAISGGKPHITVRPNSTSVQVGTI
                     TTDFNGVDGWIIDNIVVPLAQGSLRNALANAIQGFIVNNFNTTLDGLMSGLDITTLGA
                     TFNVPRIAGTGTVPLSFSVGFTSLNTTSSRLLFGIGTRFTSSVSPNAFPTLGIAQPPG
                     TNLSDMTVGSPSNTGLAAHVSVINGALHALWKANYFQATVNGSSLGSSGTTIDVTTRL
                     PPVAFISNANVVQLHIGAADLMINGNPDLPANLAARLGVEAHASVSLVGNDLVFGGII
                     IDQVHASTDTVNLTSQAQQSLQTLLAGLAQQLVNQSLNNSLPALPIPGFTIPASLTVY
                     GLPAGKQLGINSPTLSVAPQHFTLRGQFGIRP"
     gene            complement(16550..18682)
                     /locus_tag="VMZ53_22865"
     CDS             complement(16550..18682)
                     /locus_tag="VMZ53_22865"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31379.1"
                     /translation="MFVCAECGASQPMPGHCGADGSPLQTVGDDPILGATIGAYRVAR
                     LLGIGGMGRVYKGVHPTIGSRVAIKVLSRECSDRRDLVDRFFAEAKAVNLIRHESIVN
                     VLDLSTLPDGRPYIVMEYLDGSALANVIDSGLRSGQPLPIGGVARLAAEVLDALGAAH
                     AKGIVHRDLKPDNIFVTPAGRAKVLDFGIAKLQPELGGSSTHTGSLMGTPHYMSPEQA
                     AGRSVDLRADLYAIGVILFECTTGQKPFTAPALFDLLRMHIEAAPPSPRALRPDLPVA
                     LEQVIYQALAKHPEQRFASAQAMSMALQQATAQLPADQWAPIVGAASSRIPSTGGGWA
                     PTPPASWAGSQRPLSHVPSSTQTAGQVGSEARRKPASSKKGLWIALGALLLIGGGVTA
                     IVVAGGSDAKTTTASETPAPAPDPAPAPTPTPAPPSPSPTPAPPADDPWGADDPVAEA
                     LINDMLGKMPPEQRKMLPKPVQDLLKKYGSWAKVPKAERDKLGVDLAGMMAQMQIGAM
                     DDINDALTVDISNNADDPTHGTGLGPDGWVMNRTFAAYAGYDPKKVDPAKTQAFIVAE
                     AKRMVPDAVLFRIDADGVFPDGHADFSLIENGSIDYRFISPSRMKRDPKKPLGAKVER
                     RCMFRIELTKDGAWSAPMDGWDCNERLVGLPKCSFAQVWKKALAKKAPSNAIGSLGYR
                     ADFNGVAKWYFDIEDPDAKFSEMFNDDC"
     gene            18830..>19114
                     /locus_tag="VMZ53_22870"
     CDS             18830..>19114
                     /locus_tag="VMZ53_22870"
                     /inference="COORDINATES: protein motif:HMM:NF042609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HUS31380.1"
                     /translation="MTQLLDDDRPRERLWQHGVATLGDAELLAILVGTGIRARPALAV
                     AADVIASVGGISQLARASPRELALTAGIGTARATRIAAAFELGRRALTSSQ"
BASE COUNT         3297 a         6377 c         6204 g         3236 t
ORIGIN      
        1 gggatcgacc ggcgcgaaga cgcgccgctc gaccccggcg caacggcgct tcgcgccgga
       61 agagccgacg acgatccgtg ggcgacattt gcgcgcgtca ttgcgtcagg gtccggatgc
      121 gctcggcgag ccaggtccag cgcgtgcgcg cggtcgcgtt gcggaccgcc aggccgatcg
      181 cgcggcgcta gccgacgatc acgaccagct tctggccgcg ggtgaccgcg gtgtagagga
      241 ggctgcggcc gagcatcatg aagtgttgcg tgacgagcgg gatcacgacg gccgggtact
      301 cggagccttg gctcttgtgc acggagacgg cgtacgcgtg gacgagctga tcgagctcgg
      361 cgcgctcgta cgggacgatg cgaccgtcga agtcgacgcg gagcgtgccg gcgtcgtggt
      421 cgatcgcttc gatgacgccg atgtcgccgt tgaagacgtt gcggtcgtag tcgttcttca
      481 gctgcatgac cttgtcgcgc gggcggaacg tgcgatcgcc gcgcacgagc tcttgggacc
      541 ccggcgtgcc ggtgttgagg cggtcttgca gcgcgcgatt gatcgaggcg gtaccgaggt
      601 caccgcggtg catcggcgtg aggacttgga cgtcggagat cgagtcgagc ttgaagcgcg
      661 cggggatgcg ctcggcgacg agctcgacga tcgtatcgcg ggcggcgttc ggatcgtcgc
      721 gcgtgatgaa gtagaagtcg ctgtcgccgc cgggcgcgct gtcgagatcg gggagctcgc
      781 cgcgattgat gcggtgggcg gacacgacga tcttcgatgc ggcggcctgg cggaagattt
      841 cggtcaggcg gatgaccgtc gcagcgccgg actcgatgac gtcggcgaga atggcgcctg
      901 cgccgaccga gggaagttgg tcaacgtcgc cgacgagcac gagctgcgcg cccgggcgga
      961 gggcgccgag gaccgcgcgg aagaggagcg cgtcgaccat ggaggcttcg tcgacgacga
     1021 gcatgtcggc gtcgagcgga ttttcgcgcg aacgttggaa gccaccctcg ttggggttga
     1081 actcgaggag ccggtggatc gtcatcgctt ccgcggcggt tgcctcgccg aggcgcttcg
     1141 cggcgcgacc cgttggtgcg gcgagcgaga cgcggcgact gccgaggcgc gcgaggtgca
     1201 cgatcgcttt gacgatggtc gtcttgccga cgccgggacc accggtgatg accacgcagc
     1261 gatcgcgcag cgacgcttcg actgcttgcc gctgctggcg tgcgagctcg aggccggtga
     1321 cggactcgaa tgcgtggacc gcggcggcga tatcgagcgg gaacgagcgc gccggcgtgg
     1381 cggcgatttc ggcgaggagc gcggcggact cgtgctctgc cgcgtagaga tcgggaagcg
     1441 cggtgcatgg gccgcggtga ccgaggacct cgcgcacgac tttcttcgaa tgttcgagcg
     1501 cgccgagccg cactgggagc ttcgcggcgt cgattcccag cagagcggct gccgcagcga
     1561 cgagctcctc gtcgggcaga tgcatgtggc cgtcttcggc ggatgtctcg agcgcgtgca
     1621 gcaggcctgc ctcgagccgc tccggcgcat cgcgcgcgat gccgagcttt tctgcgatcg
     1681 cgtcggcagt gcgaaagccg atgccccaca cttcgtgcgc gaggcgatac gggttcgcgc
     1741 gaacgacgtt gatcgcgtcc ttgccgtact ggcgaacgat gcgcgcggcg gacgccatcg
     1801 tgatgccgtg gccctgcagg aagacgagga cgtcttgcac gtgcttttgc gccgcgattg
     1861 cgtcggcgac ttgcttcgcc ttcgccgttc cgatgccggc gatctcggtc agccggtgcg
     1921 gcgatttgtc gatcacctcg agcgtgtcct tgccgaactt ctcgacgatg cgttttgcga
     1981 tcgcgggacc gacgcccttg atgagtcccg aggcgaggaa tcgctcgatc ccgatgacgg
     2041 ttttgggcgt gcgcggatgc gcgaacgaga ctttgaactg gcggccgtac ttcttgtcct
     2101 cgacccactg gccgtggatc gtgatcggca tgccttcgtt gatctcgacg agcttgccga
     2161 cgatcgtgat cgcagcccca ctgtcgggcg caaaccgcgc gacggtgtag tactcgtcgc
     2221 cgcggaacgt gatgcgttcc agcgtgccgt cgagggtgag ctcgggctcg ccagccattt
     2281 gcgcagtcta gcgccaaagc tgttacgctc gatccgatga ggcgatctct cgtcgtcctg
     2341 gcgttcgtcg gcgcgtgtgg tggcatcgat ctcggcgagt accccgacga ggcggtcgac
     2401 gcgcgctgcg agtacctcgt caattgcggc ctgttcgagg acgccaacgc atgccgcgcc
     2461 tactaccagg atcggctgcc gaacaatccg agcgtcgagg cggcagtcga tgacggcaag
     2521 ctcgcgtacg acggcgacgc ggcggacgat tgcctcgatg cgctgcgatc gggttcgtgc
     2581 tcgcgtggcg ccgagctgcc cgacacgtgc gaccacatct tcacgggcaa ggtcgccgac
     2641 ggtggcgcct gtgcgttcga tgccgagtgc gtgtcggact tctgcgacgt cagcgactgc
     2701 acgatggctt gctgtccagg tatgtgctcg ccgtcgcgcc ccgtcccgaa gatcggacag
     2761 caatgcaact tcatctgcga ggacggcgcg tactgcggcc tcgataacgt ctgcaaggcc
     2821 gtgctgccga agggcgcgtc gtgcgattac ccgctcgcct gcgatcgcgg tctctactgc
     2881 gcggggatga cgggctcgac ggcgggcatt tgcacgcaga tgccgaagac cggcgaggcc
     2941 tgcacggaga tctgcgacga catcggtgat acgtgcaaag ccggcacgtg cacgccggtc
     3001 ggtctgtcag gcgatccgtg cacgaccgac gacgactgct ccgtctacta cgagtgcgac
     3061 ggtacaaagt gcgtcgtgcc ggcgtcggat ccgaagatgc cgaacggctc gcagtgctcg
     3121 agctcggtgc aatgccagtc gcattactgc ggcaacgaca atctctgcgc cgacgttccg
     3181 ctctgtatct agtgtttgcg ggcggccttc ggctgcgtac cgtcgacgta ttcgaccgcg
     3241 ttctggcagg acaagctctc gtacgccatc agcgagtcgc gatctgaaaa cacatcgggg
     3301 cactcggtct cgcagcgcgt tcgatcttca gcgcgcgcgc cgggtttgca atcgacgtag
     3361 tgcgcacacg cgccgttgca cgtgcccgtg agccgcggcc cgctcacgag gtgccgcacg
     3421 atgcagcccg tcaacaacag cgcgatgatc gcgccgtaca acccggactt catgagcggc
     3481 tcccgtgcct ccccgcgccg cccgcgaacc gcatcgctcc ttcgacagct tcttgcagcg
     3541 cgaccgagcc gagctcgagc tctttctgca gcgcctgctc gagcgacagg tcatgctgca
     3601 gatacgcgct cattcgatcg gcgcgcatcg ccgcttgtgg gagctccgcc agctccattg
     3661 cgagtcgctc cgctgcggcg cgcgcttcgc ctttcggcac gacgcgattc acgaggccca
     3721 tcgcgagcgc ttcctgcgca tcgacgccgc gtcccgtgag aatgagatcg agcgcgcgcg
     3781 agagcccgat caagcgcggc aggcgaatcg tcccgccatc gatcagcggc acaccgaacc
     3841 gccggcagaa cacgccgagc acggcatcgt cggccgcgac gcgcaaatcg caccacagcg
     3901 cgagctcgag gccgcctgcg acggcgtggc cttcaatcgc gccgatgacc ggcttgccga
     3961 gcagcatcct cgacggtccc atcggcccgt caccgtcggg cgcaacgcga ttcggcgtgc
     4021 ccttcgcgag cgctttcaga tccgctccgg cgcagaacgt gccgtgatcg ccgtacagca
     4081 cgccgacgcg cgcttcaggg tctgcgtcga actcgcggaa cgcatcggcg agagcctcag
     4141 ccgtcgggcg gtcgaccgca ttcttggcct ccgcacggtc gagaatcacc gtcgtcaccc
     4201 ggccggagcg ttcgactcgg accattggcc gactgtagca tcacaccgat gaagcgcatc
     4261 gcggtcctga ccagtggtgg tgacgcgccg ggcatgaatg ccgccatccg tgcggcaact
     4321 ctcatcggcc gcgcaggagg agccgacatc ctcggtgttg aacggggata tcgcgggctc
     4381 ctcgatgacg cgttcgtcga gctcggacca caggatgtgg cgggaatcct gcgcgagggt
     4441 gggacgatgc tcggctcggc gcgctgcaaa gagtttcacg accgcaaggt ccgcgatcgc
     4501 gcacgagaga tcctcgcaaa gcgcgaggtc gacggcctca tcgtcgtcgg cggcaacggc
     4561 tcgctcaccg gcgcgctgca tctcgcggac cccgctgaga ttggcagcgg ccgcccgctc
     4621 aaggtcaccg gcgttcccgc atcgatcgat aacgacctcg gtctcaccgg cctctcgatc
     4681 ggtgtcgaca ccgcgatgaa cacgatcgtc gaggcgtgcg acaagatcgc cgacaccgcg
     4741 acggcgcacg accgcacctt catcgtcgag gtgatgggac gcgactgcgg ctacctcgcg
     4801 atgacatcgg cgatcgccgt cggtgcagac ctcgcgctgt ttcccgaggc gaacaagccc
     4861 gagaacgaga caatccaaca gatcgtcgac accgtgctcg ccgttcgcgc gaagcagaag
     4921 cgcgcacgca aggtcctcat catcaaggcc gagggcgtca acatctcggc cgacaagctc
     4981 aagctcgcgg tcgacgcggg gctcaaggag cgtcagccgg atgccgagcc gagctcgatc
     5041 gagacgcgcg tgacggtgct cggccatgtc gtgcgtggtg ggcgaccaag cgcattcgat
     5101 cgcctgctcg ggagtcgcct cgcaaatgta gcggtccgtg cacttcttgc aggtgagagc
     5161 cacaagatgg ccgcgtggat gccgcccgtc gagcttccgg cggaggttgg ctctcggtcg
     5221 ccggctgatc cgtactgctg gctcgtcgat ttgaaggccg tgctcgctga gactgaaaat
     5281 ttactgaaag gacggagccc gctcgctcgg tggagagctg gcgcgttcga agagatcgag
     5341 gacgcgctgc tgttatagtg gccgtccctt gcctaccgaa cccgagctgt tgcgcctgtt
     5401 cgaccgctcg ccgatcggga tgtatcgctc cgatgcgcgt ggccggctcc tcgtcgtcaa
     5461 ccccgcgctc gtcgcgatcc ttggctacga caccgtcgaa gaagtgctcg cgctcgatct
     5521 gctcaaggac gtctacatca atgcggccga gcgcgcgccg gtgctcgagg agtatcgcga
     5581 caccggcttc gtcgaaggtc gtcgcgtgca ctggaaggcg cgcaccggtc ggattctcac
     5641 cgtccagata ttcggtcacg tcgtcgagac caaagacggc ctcgtgttcg atgccacggt
     5701 catcgacctg accgagatcg atgcgctcga agacgagcta cgccggcaac gcgaaatcct
     5761 cgagcagtcg atgcggcaga tgcccgcggt ctattgggta acggatcgcg agcagcgctt
     5821 gacgcacgta ggaggaccga cggagcagct cttcggtttt ccgcccgagc gctttctcgg
     5881 taagaagatc caagacgcgc tcgtcgatcc caaggatgat atcgacgccg tccgcaatca
     5941 cgatcgcgcg ctcgcaggcg aaattatcca gcacgaatcg ctgtacgcag cgaagctagt
     6001 ggcctcgacc acggcgccgt atcgcaacgc gaatggggac atcatcgggg tcatcggcac
     6061 gctcgtcgat gtgacggcct ggcgcgcact cgagcgccgc atggtcgacg cgcagcgagc
     6121 agagagcctt ggcgtcctcg ccggcggtct cgcgcacgac ttcaacaatc tcctcgtcgc
     6181 cgtgctcggc aacgccgacc tcgcgctgcg cgagattccc gtcggcaaac caggtcgcac
     6241 cgcaatcgag aacatccgcg tcgccggtct acgcgccgcc gagctcaccg atcagctgct
     6301 cgcatatgcc ggccgcggcg gtgcggggac gacacgagtc gagctcgcga gcatcgccga
     6361 agagctgctt cgcatcgcgg cgccacagtt cccgccggaa gttcgcgtcg cactcgacat
     6421 cgaaacgaag ctcgcggtgc ggggcgatcc tgcgcaggtt cgtcaggtgc tgatgaattt
     6481 gatctcgaat gcgcgcgagg cgctgctcgg gcgaggtggc aagatcgcgg tgacggccga
     6541 gacggtgcac agcgcaggcg acgccgacct cgacgacatg ctcaccgcgc cgaagggctc
     6601 gtacgtcgcg atccgcgtcg tcgacaatgg tccgggcatg gatcgcgaga cgcgccgcca
     6661 catcttcgag cctttcttca cgaccaagca gggcggtcat ggccttggcc tcgccgcggt
     6721 gctcggcatc gtgcgtgcac atggtggtgg cgttcgcgta cgctccgagc ccgatgccgg
     6781 cgtcgagatc accgtgctgt ggccggcggc cgtgacggct ccgatcgccg ccgcgcagcc
     6841 gccgacgacc gcaccgaccg tgctcgtgat cgacgacgag gcactcgttc gtgatgtcgt
     6901 cgcgcgcatg gtcgaagacc tcggctactt ggcgatgacc gctgccgatg gccaaaccgg
     6961 cctcgcactc ctcgataagg agacgatcga tgcagtgctc gtcgacctca cgatgccgca
     7021 gatgagcggt gccgacgtgg tcgccgccgt gcgcaccaag cggcccggac tgccggtcgt
     7081 catttgctcc ggatacgacc gcgacgggcg gggcccggtt caggctgatg cgtatttgcc
     7141 gaagccattt cgcctggacg ctctagagcg cactctggcg aaactattgc cattacgaag
     7201 tgtgtagggg ccttcgcccg cgcgcgtgga tgcgtatgct gtcttcgtgt cggaagcggc
     7261 cgcgtccacc atcaaagtcc tcctcgtaga ggacgatgcg cgtctggcgc agctaaccat
     7321 tcggtatctc gaaggtcatg gcgtcgtggt cacccacgcg agcgatggca tcgaaggcca
     7381 agccgaagcg ctgcggcgtc aatacgactg cctcgtgctc gatctcatgt tgcccggtcg
     7441 cgatggcatc gaggtctgcc ggcaacttcg ctcgcggatc gacgtcccga tcatcatggt
     7501 caccgcgcgc ggcgaggaat ccgatcgcgt gctcggcctc gaggtcggcg cggacgatta
     7561 cgtcacgaag ccattttccc cgcgcgagct gctcgcgcgc atcaaagcaa acgtgcgacg
     7621 ccagcgcggc caagccggtc ccgtcgcgca gacgatccaa gtcggcaagc tcgtcctcga
     7681 tccagccgcg atgacagtcg tgctcgacgg caatcccgtc gacgtaacgg cgtacgagtt
     7741 ctcgatcttg cgcgcgctcg cgcagcgtcc cggcaaggtg ctctctcgcg agcagctgct
     7801 cgatctcgcg aagggctcgg cggacctcag cttcgatcgt tcgatcgacg tccacatctc
     7861 ccgcctgcgc aacaagctcg gcgatgacag ccgcagcccg aagattctca agaccgttcg
     7921 cggcgtcggg tatctcctcg ccggagggac cgagtgagca agcgcaagct cgcgctgcgg
     7981 ctctacgcgc tgacggcagc gatcgcgatc gtcacgatgg tcgcgctcct cctgctgccg
     8041 cgtttcgtcg gtagccctcg ttatctcgag ccgcaggccg cgctcgtgca gaacctcgtc
     8101 gatcgctttt cgctgcgcga gccggagcgc cgcgccgagc ccctcgagcg gtggcgcaag
     8161 cgcctcaaag gcaagctcac gttctacgac acacaaggca gcgtcatcgc atcgacacac
     8221 gaggagctac caccgccgac aaccgacgag ctcaaaggtc tcgaaggcga tcagaagtgg
     8281 atcctcgaat accggcgcat cgtcgtccgc tccgacgacg gcaaaatgat cggcgtctac
     8341 gttccgaaca cgcccggctt tccgtggggc ttcgtccttc ccctcggcgc gggcgtgctg
     8401 atcgtcgtcg gtgcggcaac gctgtggttc tcgcgacgcc tcgtgcgccc cctcgattca
     8461 ctcgcgatgg cggcgcagaa gttcggcagc ggcgacatct cctcgcgcgc gcacctgcat
     8521 cgcgatgacg agctcggcga cgtcggtaaa gcattcgacg atatggccga tcgcaccgca
     8581 gcactgatct cgtcgcagcg tcagctcatg gcggatgtct cgcacgagct gcgcacgccg
     8641 ctcgcacgta ttcgcgttgc actcgagctc gcagctgaag atccgttggc cgcgaaggat
     8701 gtgctcaccg acgtcggcac cgatctcgac gagatcgatc agctgatcgc agacatcttg
     8761 accacggcgc gtctcgatgc ggatccggcg cccgtcgcgc gcgagccgat cagcgtcggc
     8821 gagcttgccg atcgcgcagc gtcgcggttc acggcgcgcc atccaggccg cacgctcgag
     8881 aaaaccgtcg acgatgcaga acgtgcgatc gaatgcgatc ccgtgctgct gcgccgcgct
     8941 ctcgacaatc tcctcgataa cgccgcgaag tactcggaag cggacaagcc gatcacgctc
     9001 gccgttcatc cgaatggcaa ggccgtcgat ttcgaggtgg tggacaaagg catcggcatg
     9061 tccaaggacg agctcgcacg cgcgggaaca ccgttttggc gcgcggacgg tagtcgcgct
     9121 cgtcgcacgg gcggcgtcgg cctcgggctt gcactcgctc gccgcatcgc gcgtgcacat
     9181 ggtggcgaca tctctctcga ttcagttccg ggacgcggta cgaccgttcg gcttcacgtg
     9241 ccagtaggtg agtcgtgatc aaacgttggc ttcctcgcat catcgttctc gcgatcgtcg
     9301 ccggtggcgt cgcgttcctc tggatcaaga aaccgtggca gaaggcgccg acgcccgtca
     9361 cgttcagcac cgttcccgtc acgaagggaa cgatcgccgc gcaggtgacc gcgaccggca
     9421 cgctgtcggc gcgcggtacg gtgcaggtag gcgcgcaagt ctccggccgc gtcgtcgagc
     9481 tcaaggctga cttcaacgac aaggtcaaaa agggccagat catcgcgaag ctcgacgaga
     9541 gcgtgctcaa ggcgcagatc caacagcagc aagccgccta cgatctcacg gtcgcgaatc
     9601 tcaagaagtc gcaggtgtcc gcgatggacg cgaagcgcca actcgagcgg cagaggacgc
     9661 tcaaggatca gaacctgatc gccggcgcga ccgtcgagca gtacgaagtc acgctcgaga
     9721 ctgcgaacgc gcagatcgtc gcctcgaagg ccgaagtcgc acgcgccgcc gcaaacctcg
     9781 agcaagctcg cacgaatctc ggttacgcga cgatcatctc gccgaccgat ggtgtcgtgc
     9841 tctcgcgtgc tgtcgagctc ggccagaccg tcgcgtcgtc gttgcaggcg cccgtgctgt
     9901 tcacgatcgc ggaagacctc acgcgcatgc agatcgatac ggcggtcgcc gaaggcgatg
     9961 tcggcgcgct gcgcgaaggc atgaaggcgc agttcagcgt cgatgcgttt cccggcaaga
    10021 cgttcgaagg cgtcgttcgc caggttcgca atgcgccgac gaccacgcag ggtgtcgtga
    10081 cctacgacgc cgtgatcgat gtcgataaca ccgatctctc gctgcgcccg ggcatgaccg
    10141 cgagtgtcac gttcgtgctg cagtccgtcg agaacgtcgt caagattccg aacgccgcac
    10201 tgcggttcaa gctgtcgcgc gatcagatga tggcgctcgc ggagaagtac aacctgccca
    10261 tgggccgtcg cggcagcggt gccggtggcg gcagtggcag ctggtccggc cgtcgcggca
    10321 gcggtgcggg tagcggtggc ggcgagctcg cgcaaggctc gggcgcgtcc ggcggcggtg
    10381 gtggccccgg cggcggcggc ggtggccccg gcggcggtgg tgaaggcggt ggacggcgca
    10441 tgggcgatcg caagcagctg tggaagctcg tcgacggcaa accgaagatg gtgttcgtca
    10501 agaccgggct gaccgatggc tcgacgacgc agctcctcga cggagacatc caacccggcg
    10561 atcagctcat caccgagatc caaggcctgc ccgcgccgaa gcgaaagatg ggagcgttct
    10621 gatggcggcg cccctcgtcg agatgcgtgg catcagccgc acgtaccaga tgggcgacaa
    10681 cctcgttcac gcgctgcgca acgtcagcat cacgatcgag cgtggcgcgt cgatcgcgat
    10741 catgggcacg tccggatctg gcaagtcgac actcatgaac atcatgggct gcctcgatca
    10801 accgacgagc ggtgagtatc tattgagcgg cggcgacgtc tcgaagctct cgcgcagcca
    10861 gctcgcagat gttcgcggca agatgatcgg gttcgtgttc cagagcttca atctgctcac
    10921 ccgcacgacc gcgctcgaga acgtcgagac accgctcatc taccagggag tcagtgctcg
    10981 tgaacggcgc aaacgcgcga tcgaagcgct cgagcgcgtc ggcctgcacg gacggcacga
    11041 ccacacaccg aatcaactct ccggcggcca gcagcaacgt gtcgcgatcg cgcgtgcgct
    11101 cgtcacgaag gctcccgtca tcatggccga cgagcccacc ggcaacctcg acaccaagac
    11161 gtcgtacgaa gtgatggcga tcctgcaggg cctcgtcgat cagggcatca ccgtcgtgct
    11221 cgtcacgcac gaagcagaca tcgcggcgta cgccaggcgc gtggtgacgc tgcgcgacgg
    11281 cgcgatcgtc gaagacaaga cgcaagagcc ggtgaaggcg gtcgcatgaa catcttcacg
    11341 acgttcttgc tcgcgttgcg cgcgctgtgg cgtcacaagg tgcgcacgtt cctgacgatg
    11401 ctcggtctca tcggtggcgt cggcaccgtg atcgcgatgg tcgcggctgg acagggcgcg
    11461 cgcgagtccg tcgccgacgt cttccgatcg atgggcacga acctcctcat catctcgaac
    11521 ggttcccagc gccaaggcgg cgtcgccggc ggtaccggct cgtcgttcgc gctcacgtgg
    11581 gacgacttcg cggcgctcga gaacggcgaa gtctcgacgg tgcgctgggt ctctcccgtg
    11641 ctcgcgacga agatgcaggt cgcggcagag gatacgaact ggaacacgca ggtaaccggc
    11701 acaaacgctg cgttcttcaa gatcaagaac tgggacgtca ccgagggcaa gctgttcgat
    11761 gacgacgtcg gtccggcgaa gatcgcgctc atcggcaaga ccgtcgcaac gacgctctat
    11821 cccggtgtga atcccatcgg ccaagcgatc cggatcaacg gccagccgta cgaggtcacc
    11881 ggcatcctcg cttcgaaggg ccagtcggcg atggggcagg atcaggacga catcgtggtc
    11941 gtgccggtga agacgtatct cgcaaagctc gaccgtggcg ccggcaagtt catcacgcgc
    12001 ggtcagatct atgtctcgac ggaatcggag aacgacatca cgcgtgcgga gcagcaaatc
    12061 agcgagctgt tgcgtcagcg acacaacctc ggtcccaacg acgaggatga cttccgcatt
    12121 cgcaacctcg cggagttcgc gctccgtcag aaggaatcga cggacaagat cacgacgctc
    12181 ctcgcactgg tcgctgcgat ggcactgctc gtcggcggca tcggcgtcat gaacatcatg
    12241 ctcgtcagcg tgatcgagcg gacgcgagag atcggtattc gtatggccgt cggtgcaaag
    12301 ccgctcgacg tgatgacgca gttcctcgtc gagtctctga tgctgtcgct gatcggcggt
    12361 gccatcggac ttgcgatggg cgcctggtgg gccaagctca tggcggattt ctacggttgg
    12421 aagtttctct ttcccgctca gaccgcggtc atcgcgttct tgatctcgag cggtatcggc
    12481 gtggtcttcg ggctctatcc cgcgattcgg gcgtcgttgc tcgatccgat cacggcgctg
    12541 aggtacgaga catgaagtta cgaatcgccc tttgtcttgc attgctatcg tcgcccgcac
    12601 tcgcacagcc accgccgcag acgctcacga tggagcaggc tgtcgagatc gcgatgaaga
    12661 gccagcctgc gctgcggcag agtcgcgcgt cactcgaagc ctcgcgtggg cgcatcgagc
    12721 aggtcgaagc gacccgtcgg ccgacgctga atctatcggc gagcgcgaac atcggcggtg
    12781 gtggcccatc gcgcgacatc atcgatccgt cggcgagctc ggcgatcggc gcgagcgcgc
    12841 agtggcgcat caccgacttc ggtcagactg cggcgcagtt gcgtgccgcg cagctcagcg
    12901 cagaggcgag ctcggcggat gtgcagaccg atatcctcga cgtgcgtcgc gatgtcgagc
    12961 tgacgtttct ggaagcagtc gcgcgcacgc gtttgctcgt cgtcgccgaa gcgaccgtga
    13021 agagcgagga gacgcacctc gaccaagcga agcggttcgt tgcggcgcag gcgaaagatc
    13081 cgatcgaggt cgtgcaggcg caggctcgag ccgcgaatgc gcgcgcatcg ttcgcgcaag
    13141 ctcagtcgca agccgcgatc gcgctcgcga acttgcgggc cgccatcggc tgggtcgatc
    13201 cggcgtcgac gctcgcggtg tcgaacaact ggcccgttcc tcccgatgcg ccgccgcccg
    13261 tgctcaccga gctcgtcacc acggcgcgct cgcaccgtcc ggagatcgtc gcgctcgaca
    13321 agcaggtcgc cgctgccgaa gcgaacatcg atgcggctcg cgctcagcgc cggccggtgc
    13381 tctcggcgaa cgcgcagagc cagtggtcac cgagctggtc gaaggaagcc gacatgaatg
    13441 gcgaccacat caatgttcca cagccatcgt ggagcatcgg gctgtctctg acctggcaag
    13501 cctatgacgg tggacgcacc gccgcggacg tccgcgtggc gcgggcgaac cgcgagtcgg
    13561 ccctcgcaca acgcgatgca ctcctcctcg atctgacgag ccaactcgac tcggcgcgtg
    13621 cccagatcga cgcgaaccgt gcggcaacac aggcttcgac ggaagcggtc gaggcggctc
    13681 gcgcgcagct cgagcttgcc gaggcgcgtt acgcccaagg actcggaagt cagatcgagc
    13741 ttgcggacgc ccagaccgcc gtcacgaccg ccgaaggcaa tctcatccag tcggaattcc
    13801 agctggccac tgcctgggca acgttgcgtc gatcactcgg cgggatttag gcaattccag
    13861 ccacatgccc tgataccatc ggggcgatgg ggattcgcgc gttctggccc tccgcctttg
    13921 ctctgggcat cacgctcctc gccgcgtgtg gcgacaacct cgagccgccc gacgatccag
    13981 atcagctcgt caacgtcgag ctcgagaccg gcgcgccctc gcaggtgatg gccggcgaca
    14041 cgatcaacgt gacgtgcacg ctgcacgagc gcgacatgaa cggcgaagac ctcgcgacga
    14101 tggttcccgc cgagatcaag atcgtcgatg agacgaaggt ctcccgcctc agcggaacga
    14161 tcatcgcgcg caaggtcggc atcatcgcgg tctcgtgcca actgcccgat cgcggcgtcg
    14221 cggatgcatc gccggtgatg gtcgaaatcc tcccgggcgc tgcgtcgaac gtcgtcacta
    14281 ccgtcacgcc gaatcccgtc gtcgcaggta acgacatcgc cgtgacgtgc acggtctacg
    14341 acgactacgg aaacctcgtc gagggccaat cgccgacgct cgcactcgcg ccggacgaca
    14401 acgcgaatac cgtcgccggt ctcggcgcga cgatgatcca ctccggtcac tacatcgcga
    14461 cgtgccagct gcctggctcg acgagcaaca acgcaggctt cgacgtgctg ccgaatctgc
    14521 cggcgaacat ctcgctgtcg cgcgcgcccg atctcccggt ctacgcgatc aacgacacga
    14581 tccagatcct gcacgtcatc accgatcgat acggcaacga gatcttcgac gcgacggtca
    14641 ccgatgtgag cacgccgctc gtaggcacag gcccgacgac gtatctcggc gcgtcgacgt
    14701 tcaagtacgg cggcgaaggc acgttccgca tcaccgtgac ggtcgatcct ccgacggacg
    14761 gcggcgtcac cgtcagcgcg tcgatcaacg ttctcatcaa cagccgcggt ccagcgatca
    14821 gctgtctcaa cgacgcgacg atgctgaacc tcacgccggg cggcacgtac accgtcaacg
    14881 gcaacgcgaa tgacgtcaac ggcgtgaagt cgctgaccgt caatggcaat gcagtcacgt
    14941 tcaacggcaa cggcgactgg accgcaaaca tcacgacgcg gttcggcatg aacttcgtcg
    15001 acatcaccgc gatcgataac ttcgacgagc cgacgacgaa ggtctgcacg ttcctcgtct
    15061 cgaatcgcta cgcggcgacc acgacgccaa tccccgacgt gctctcgctc aagctcacgc
    15121 agcccgccgt cgacgatttc aatcgcacga acacgctcgg ctcgttcggc gacatcctgc
    15181 atacgatcgc gaatagccag ggtctcaggg acgcggttca caacgcgctc tacgcgaaca
    15241 acccgctcaa gccctcgagc tgtgactcgc agacctgcgt gccgttcatc ggctgcgcgt
    15301 gctggtacag ctccggcgtt tggtacaact actcgcagtt cgacggtccg aacacggtgt
    15361 cgctcacgct cgtcgatggt ggtatcgccg caacggcgcg cctcgagaac gtgcacgtca
    15421 acctgcgcgt ctggggcaag gtcggcccga ttccgtacga cacgaccggt tgggtgaacg
    15481 tctcctacgt ccaggtcaac ctgactctcg ataccgcgat ctccggcggc aagccgcaca
    15541 tcacggtgcg cccgaattcg acgagcgtgc aggtcggcac gatcacgacg gacttcaacg
    15601 gcgtcgacgg ctggatcatc gacaacatcg tcgtgccgct cgcgcaaggc tcgttgcgta
    15661 acgcgctcgc gaacgcgatc cagggcttca tcgtcaacaa cttcaacacg acgctcgacg
    15721 gcctgatgtc aggcctcgac atcacgacgc tcggcgcaac gttcaacgtg ccgcgcatcg
    15781 cgggtacggg cacggtgccg ctctcgttca gcgtcggctt cacgtccctc aacacgacgt
    15841 cgtcgcggct cttgttcggt atcggtacgc ggttcacctc gtcggtcagc ccgaacgcat
    15901 tcccgaccct tggcatcgcg cagccgcccg gtacgaacct cagcgacatg accgtcggct
    15961 cgccgtcgaa caccggtctc gcggcgcacg tcagcgtcat caacggcgcg ttgcacgcgc
    16021 tgtggaaggc gaactacttc caggcgacgg tcaacggcag ctcgctcggc tcgagcggca
    16081 cgacgatcga cgtcacgaca cggcttccgc ccgtcgcgtt catcagcaac gcgaacgtcg
    16141 tccagctgca catcggcgcc gcggatctga tgatcaacgg caatcccgat ctgcccgcga
    16201 acctcgcggc gcggctcggc gtcgaggcac acgcatccgt atcgctcgtc ggcaacgacc
    16261 tcgtgttcgg cggcatcatc atcgatcagg tgcacgcgtc gacggacacg gtgaacctca
    16321 cgtcgcaagc gcaacagtcg ctgcagacgc tgctcgctgg gctcgcgcag cagctcgtga
    16381 accaatcgct gaacaactcg ctaccggcgc tgccgattcc gggcttcacg attcccgcgt
    16441 cgctcacggt ctacggtctg cccgcgggca agcagctcgg catcaactcg ccgacgctgt
    16501 cggtcgcgcc gcagcacttc acgctgcgcg gtcagttcgg cattcgccct taacagtcgt
    16561 cgttgaacat ctcgctgaac ttcgcgtcgg gatcctcgat gtcgaagtac cacttcgcga
    16621 cgccattgaa gtccgcgcgg tagccgagcg agccaattgc gttcgagggc gctttcttcg
    16681 cgagcgcctt cttccagact tgcgcgaacg agcacttcgg aaggccgacg agcctctcgt
    16741 tgcagtccca gccatccatc ggagcgctcc acgcgccgtc ctttgtgagc tcgatgcgga
    16801 acatgcagcg gcgctcgact ttcgcgccga gcggcttctt cggatcgcgt ttcatgcgcg
    16861 atggcgagat gaagcgatag tcgatcgatc cgttctcgat gagagagaag tccgcgtggc
    16921 cgtcggggaa cacgccgtcc gcatcgatgc gaaacagcac ggcgtcgggc accatgcgct
    16981 tggcctcggc gacgatgaat gcctgagtct tcgcggggtc gactttcttc gggtcgtaac
    17041 cggcgtacgc ggcgaacgtt cgattcatca cccagccgtc gggcccgagc ccggtgccgt
    17101 gcgttgggtc gtcggcgttg ttgctgatgt cgacggtgag cgcgtcgttg atatcgtcca
    17161 tcgcgccgat ctgcatctgc gccatcatgc cggcgagatc gacgccgagc ttgtcgcgct
    17221 cggccttcgg tacctttgcc cacgacccgt atttcttcag cagatcctgc acgggcttcg
    17281 gcagcatctt gcgctgctcg ggcggcatct tcccgagcat gtcgttgatc agcgcttctg
    17341 cgactggatc atcagcgccc cacggatcgt cggctggtgg tgccggcgtc gggctcgggc
    17401 ttggtggcgc gggcgtcggc gtcggtgcag gcgcggggtc gggcgcgggc gctggcgtct
    17461 ccgacgcggt cgtggtcttc gcgtccgagc caccggcaac gacgatcgcg gtcacaccgc
    17521 cgccgatcag aagcagcgcg ccaagcgcga tccacaagcc cttcttcgac gacgccggct
    17581 ttctgcgcgc ctccgagcca acctgcccgg ctgtctgcgt cgacgatggc acgtgactga
    17641 gcggccgctg gcttcccgcc cagctcgcgg gtggagtcgg cgcccagcct ccgcccgtcg
    17701 acggaatgcg actgctcgcg gcaccgacga tcggcgccca ctggtccgct ggcaactgcg
    17761 cggtcgcttg ctgcagcgcc atgctcatcg cctgcgccga cgcaaaccgc tgctcgggat
    17821 gcttcgcgag cgcttgatag atcacctgct cgagcgcgac gggcaagtcg ggtcgcagcg
    17881 cgcgcggaga cggcggcgcg gcctcgatgt gcatccgcag caagtcaaac agcgcgggcg
    17941 ccgtgaacgg cttctgcccg gtggtgcact cgaaaaggat cacgccgatc gcgtagagat
    18001 ccgcgcgcag atcgacgctg cgtcccgccg cctgctcggg cgacatgtaa tgaggcgttc
    18061 ccatcagcga gcccgtgtgc gtcgagcttc cgccgagctc gggctgcagc ttcgcgatgc
    18121 cgaagtcgag caccttcgcg cgtcccgcag gcgtcacgaa aatattgtcg ggcttgagat
    18181 cgcggtgaac gatgcccttc gcatgcgcag cgccgagcgc atcgagcacc tcggccgcga
    18241 gccgcgcgac gccgccgatc ggcaagggct gcccgctgcg cagcccggag tcgatcacat
    18301 tcgcgagggc ggagccgtcg agatactcca tcacgatgta cggacgacca tcgggcagcg
    18361 tcgagagatc gagcacgttg acgatgctct cgtgccggat gaggttgacg gccttcgctt
    18421 ccgcgaagaa ccggtcgacg aggtcgcgcc gatcgctgca ctcgcgcgac agcaccttga
    18481 tcgcgacgcg actaccgatc gtcggatgca cgcccttgta gacgcgcccc atcccgccga
    18541 tgccgagcaa gcgcgcgacg cgatacgcgc cgatggtcgc gccgagaatc ggatcatcac
    18601 cgacggtctg cagcggcgag ccatccgcgc cgcaatgccc tggcatcggc tgcgacgctc
    18661 cacactccgc gcacacgaac atggctccaa agcctaacac gcgcatgtcc gccgcacgtc
    18721 cgcgcgtccg cccaccggct cggcgaatcg cccgtcggac aatgacccca cctaactcct
    18781 cgacgacaca cgcgccaaac ctggcgccac gtttgctcgc gtcttccgca tgactcagct
    18841 gctcgacgac gaccgaccgc gcgaacgtct ttggcaacac ggcgtcgcga cgctcggcga
    18901 tgccgagctc ctcgccatcc tcgtcggcac cggcattcgc gcgcgccctg ccctcgcggt
    18961 cgccgccgat gtgatcgcct ccgtcggtgg catctcccaa ctcgcacgcg catcaccacg
    19021 cgagctcgcg ctcaccgccg gcatcggcac cgcgcgcgca acgcgcatcg ccgccgcgtt
    19081 cgagctcggt cgccgcgcgc tcacatcctc gcaa
//
LOCUS       DATLBZ010000453         9999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6997125, whole genome shotgun sequence.
ACCESSION   DATLBZ010000453 DATLBZ010000000
VERSION     DATLBZ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6997125"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VMZ53_22875"
     CDS             complement(<1..742)
                     /locus_tag="VMZ53_22875"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HUS31381.1"
                     /translation="MKKILLGLLITSACGGDAGEQLFVPKPECMGAQITPYSGTFPQV
                     INKLAIGSVEDGFDLDHDGKPDNKLAAVASLAQSSIDDSFKKYDILIPIEFFDFETVA
                     KDECVKFAIYLADYVTDKDMDGKKGFIDEGDCNDNVAAIKPGAQEVPGDSIDNNCNGL
                     ADEDGQGVPSDSTANADNDGVTVHDGDCDDTNPMVHKGMAEICGDGLDNDCDGVADRT
                     SDGAGGSTACSPFDGTRDLNLDPLSLMGG"
     gene            790..1560
                     /locus_tag="VMZ53_22880"
     CDS             790..1560
                     /locus_tag="VMZ53_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31382.1"
                     /translation="MTFVLGVVAITLLSGHVSPSVDDNNRYLKVTPQADGIRFAYTVF
                     FGEIPGASERKLLDANHDGQIDTAESKAYGDKLASQVASAVDIDVDGRPQKLVWSVVD
                     VGMGSREVAAGSFSVDMIAYLCGSGGTTHRVKVRDQFRVPRPGETDVKVEDSPSITVT
                     KARIGPADDATHEYRFVGPGGPLSDDGLDIEFKVVGEAPRVPDDRCGKAIAARKAEGA
                     KYIWLGVAMLLAGGALGAALLVYVRKRNASAAANRRAQ"
     gene            complement(1531..2256)
                     /locus_tag="VMZ53_22885"
     CDS             complement(1531..2256)
                     /locus_tag="VMZ53_22885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31383.1"
                     /translation="MKRYVLALIAVAACGDDGGGMVTPDSSTPMPDAPVTCPAVTPPL
                     AAGMHTFYLSFEGVTITKGACDDAKTNCSELVGQASTVVPAFLPAESDRDARIAVIAA
                     EVQTALAQFSVDVVRTRPASGDYWMASIGGTSMAVAGVSGVTAGVKPTCQSVNKSSIS
                     LVFDRGATVTDRAYADVITTTFGQLVGLVSVNLSGDCMCNAGSCTHTDACTWGTSVST
                     QINSCSRQVQNEQELLRTAVGCR"
     gene            complement(2271..2936)
                     /locus_tag="VMZ53_22890"
     CDS             complement(2271..2936)
                     /locus_tag="VMZ53_22890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HUS31384.1"
                     /translation="MAVRARLPKPSPEEVPGAESFLPVKHNLTALRAAVPTCRGCPLY
                     KAATQAVFGEGSASSKVMLVGEVPGDREDLAGKPFVGPSGKLLDEALALAGIPRDEVY
                     VTNAVKHFKFTQRGKRRLHDKPNRYEIAACKPWLDSELEVIEPEIVVLLGATAAQALL
                     GSAFRVTKQRGQALDTQLAKWTFATVHPASVLRAPDEEMRRIAKEEFIADFEVVGDYF
                     KKL"
     gene            3001..3534
                     /locus_tag="VMZ53_22895"
     CDS             3001..3534
                     /locus_tag="VMZ53_22895"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS31385.1"
                     /translation="MKTTLGIITSLLLSSAAFAGNAAPDFTGAAPRPHIDKSLVASSG
                     TAPIEPGDVLQFRSDEAILTTSGYSQVDRAALWLKVHPKDNIVIEGQADHPGTEPYNS
                     DLSMLRAEAVRKRLMSQGISRKRIIMVASGEKDQLLSPADRRVRMYATKLSPQAIYAM
                     TAEQARNAPAVEQTARR"
     gene            3562..5049
                     /locus_tag="VMZ53_22900"
     CDS             3562..5049
                     /locus_tag="VMZ53_22900"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS31386.1"
                     /translation="MKKFPDFVPPTKVSYLDRGPTLHLRRCILQTEDDSQEWVFDKEE
                     IRIGSMEDNDVVLADDTVSRYHCKIIQDDTGYVLVDNHSTNGTFINKVRVREGFLKPG
                     CSLAVGQSLLKFNAREEEVQIVPSRATRCGNLIGGNAKMREIYSIIEKIAPTATTVVI
                     DGETGTGKEVVAQSIHSLSPRSKNELVVFDCGAVPPNLIESELFGHEKGSFTGAMMTR
                     TGLFEQADGGTLFLDELGALPLDLQPKLLRALEQREVRRVGGAKSNKVDVRIIAATNR
                     NLEDEVRAGRFRQDLFYRLSVVRLHLPSLRDRADDIPVLVQHFVETGPYNRGPDGKPR
                     VRGVSREAVNALQAYPWPGNVRELVNVIERAVSFCTHGQIELSDLPDYVRTAKPAPQK
                     EHQGARPLPRQPTGGNPTVGSALSPTPPATPDELLAEGVTFKDAKERWVATFERDYIL
                     QMLRRNTGNISHAARSADIDRKYFRKLMKKYDIEAAGVDDDDAVE"
     gene            5027..6103
                     /locus_tag="VMZ53_22905"
     CDS             5027..6103
                     /locus_tag="VMZ53_22905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUS31387.1"
                     /translation="MMTTPSSEEDDADALLDDEEDAAPVDDGAGAHLDENGKPRIIER
                     HLIVTPELAGLRLDHYVKTQIQRLSRTKIQAIIETQLRRADGSAVKSSTTVALGDHFI
                     IRRQARAEPPCPRTFTVLYEDARMRIVDKPAMLPVHATAKFYFNTLTRVLWERYPDED
                     ELQLCHRLDRETSGCVVLARDKAAAAKLKGAIAAKDKTTKQYVAVVHGTPPWTEETTI
                     DLPLRLAGPADATRLPHVRMLPATMAEPDAQSAITRVVVERAGNPYSLVRCTLVTGRQ
                     HQIRAHLAARGFPIVGDKIYTHGDDAFIEYCASGLTPELAAQFVLPRHALHAARITVP
                     HPEDGRPITAIAPLPSDMATLLGE"
     gene            6231..6596
                     /locus_tag="VMZ53_22910"
     CDS             6231..6596
                     /locus_tag="VMZ53_22910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31388.1"
                     /translation="MAQSLQCPESMKDFQTLTTEELTTAHGGMSAAARFIMMHESGGS
                     TTAGHLYKQGRGDGTRGNHSSAFGAFQMIQATRKAYMGRDYQSTDFNKQYAAASHYVR
                     DRYGSWERAASFWRSHHWY"
     gene            6661..8013
                     /locus_tag="VMZ53_22915"
     CDS             6661..8013
                     /locus_tag="VMZ53_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31389.1"
                     /translation="MRRSVVAVLFGLVAACGNSSGTTGDANGGDDDQTGDDAGGTSGP
                     RTVKLTLTNRPMTAANFSFLVAYQDGSAAWQLAPAPAGDVYSFEINAPNYGVAFACIG
                     TTAGTTTQVRSVTSAYFAVGERTELTLDVPARCSDRQTTMGAVMLNGSVTNRPFNGGT
                     LVAVWGGRSAFVGTQTGNFQLTVQPGTHDLFVVHAVPEGNGDFYTDEVWVARDVTLTA
                     NTNRTINFNEAQSTTQYAVNIGTVDPAARVTASTTVYTANGTAAGLVRETMNWETSSL
                     ADAQMKTTDVYDQSIQVSSFAKSATVTHATNEPGDIDWTAPAPLGAVQSMTATKMPYV
                     ILQATWPAYADVDGYLWNAAQQLSGSQCGGNTSSACSIVWQAYLSPGVTGAMPAFRMP
                     ELADLAGWKDAFELVSGTQAFGSVTAYTSSAGAGDFPAGVPANGTTRTFVRSDFATTP
                     "
     gene            8036..8404
                     /locus_tag="VMZ53_22920"
     CDS             8036..8404
                     /locus_tag="VMZ53_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31390.1"
                     /translation="MAKKDRAAPFSKKHRRYWIPVTGGMLLIGAINVALGMCSYKEAP
                     PTERMELVIPGHERRPGQLMRAEIPADVMRAFAVKYPHTIPDGAITNGTDNTIIVQFP
                     PGNALHTAVFKLDGTFVSEQ"
     gene            complement(8405..>9999)
                     /gene="sppA"
                     /locus_tag="VMZ53_22925"
     CDS             complement(8405..>9999)
                     /gene="sppA"
                     /locus_tag="VMZ53_22925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HUS31391.1"
                     /translation="ASVLGDGDHIERVELSGTIGSRELTSIVLRLRSIAHDSTAKAVI
                     VMFDGADGGWATLQELRDELLAVKKAGKKVFAYMVSGTGRDYFVATAADKIYVDPAGG
                     VRLVGMAGETLHFRGAFDMIGVTPQFEKIAEYKSAPEQFTEKKATDTAAKMANDLYDS
                     LWDQWLTAIAAGRHLSKDEVKALVDAGPYTAGDLAKDQKLVDGVASPDKVAQMIVTEI
                     GRSYPVTSPGADRPDRWTRPGIAVIYVEGDITDGKSQTIPILGRSVAGGETLVAAVTA
                     ARNDPRVGAIVMRIDSPGGSALASELVSREIFQTRGVKPVICSFSDLAASGGYFIAAG
                     CDTIYAEPMTITGSIGIFYGKFDLSGLMAKVGVTTDTYKRGKRSDVESMFRPYTDEER
                     TALLEKLKYMYSRFVGAVSEGRKLTKEQVDGLGRGHVYTGDMAKPIQLVDKFGGLGDA
                     LDDAKKRMGLDPSTRVTIYELPKLPSSIFGPLGSLLGVKEQSSVFELPIVRSLLSGLP
                     ASLLVSPDAAQARLPYDLQFAP"
BASE COUNT         1766 a         3351 c         3201 g         1681 t
ORIGIN      
        1 cgccgcccat cagcgagagc gggtcgaggt tcaggtcgcg cgtgccgtcg aacgggctgc
       61 acgccgtcga gccgccagcg ccgtcgctcg tgcgatccgc gacgccgtcg cagtcgttgt
      121 cgaggccgtc gccgcagatc tcggccatgc ccttgtggac catcgggttc gtgtcgtcgc
      181 agtcgccgtc gtggacggtc acgccgtcgt tgtcggcgtt cgcggtgctg tcgctcggca
      241 cgccttggcc gtcttcatcg gcgaggccat tgcagttgtt gtcgatgctg tcgccgggaa
      301 cttcctgcgc gccgggcttg atcgcagcga cgttgtcgtt gcaatcgcct tcgtcgatga
      361 agcccttctt gccgtccatg tccttgtccg tgacgtagtc cgccaggtag atggcgaact
      421 tcacgcactc gtccttggcg accgtctcga agtcgaagaa ctcgatcggg atcaggatgt
      481 cgtacttctt gaacgagtcg tcgatcgacg actgcgcaag gctcgcgact gccgccagct
      541 tgttgtccgg cttgccgtcg tgatcgagat cgaagccgtc ttcgacgcta ccgatcgcga
      601 gcttgttgat cacctgcgga aacgttccgc tgtacggcgt gatctgtgcg cccatgcact
      661 cgggcttggg aacgaacaat tgctcgccgg cgtctccgcc gcatgcactc gtgatcaata
      721 gaccaagaag gatcttcttc atggggtacc ctcgccgcgg acttgagcac gatgcgtaaa
      781 gcgcggtcaa tgaccttcgt cctcggcgtg gtggccatca ccctgctctc gggtcatgtg
      841 tccccatcgg tcgatgacaa caaccgctac ctgaaggtca cgccccaggc cgacggcatc
      901 cggttcgcct atacggtctt ctttggcgag atccccgggg cttccgagcg caagctcctc
      961 gacgccaacc acgatggcca gatcgacacg gcggagtcga aagcctacgg cgacaagctc
     1021 gcgtcccaag tcgcttccgc cgtcgatatc gacgtcgatg gcaggcccca gaagctcgtc
     1081 tggagcgtcg tcgatgtcgg catgggctcg agggaggtcg ctgctggctc gttctccgtc
     1141 gacatgatcg cctacctgtg cgggtcgggc ggcacgacgc atcgcgtgaa ggtgcgtgat
     1201 cagttccgcg tcccgcgccc gggcgagacc gacgtcaaag tcgaggacag tccgagcatc
     1261 acggtgacca aagcgcgcat tggtccggcc gacgacgcga cccacgaata ccgattcgtc
     1321 ggtccgggag ggccgctctc ggacgacggt ctcgacatcg agttcaaggt ggtgggcgaa
     1381 gcgccgcgcg ttcccgacga tcggtgtggg aaagccatcg cggcgcgtaa ggccgagggc
     1441 gcgaagtaca tctggctcgg cgtcgcgatg ctgctcgcgg gcggcgcgct cggggcggcg
     1501 ttgctcgtgt acgtcaggaa gcgcaacgca tcagcggcag ccaaccgccg tgcgcagtag
     1561 ctcctgctcg ttctgtactt gccggctgca cgagttgatc tgcgtcgaga cgctcgtgcc
     1621 ccacgtgcat gcgtcggtgt gcgtgcacga tccagcgttg cacatgcaat cgcccgacag
     1681 gttgaccgac acgaggccga ccagctggcc gaacgtcgtc gtgatgacat ccgcgtacgc
     1741 gcgatccgtc accgtggcgc cgcgatcgaa cacgagcgag atgctgctct tgttcaccga
     1801 ctgacaggtc ggcttcacgc ctgccgtcac gcccgagacg cccgccaccg ccatcgacgt
     1861 gccgccgatc gaggccatcc agtagtcgcc cgatgccggc cgcgtgcgca cgacatcgac
     1921 ggagaactgc gcgagcgcgg tctgcacctc cgcggcgatc acggcgatgc gcgcatcgcg
     1981 atccgactcg gccggcagga acgcaggcac gaccgtcgag gcctggccga cgagctcgga
     2041 gcaattcgtc ttggcgtcgt cgcacgcgcc tttcgtgatc gtgactccct cgaacgatag
     2101 atagaacgtg tgcatgcccg cggcgagcgg cggcgttacg gccgggcacg tcaccggcgc
     2161 atccggcatc ggcgtgctgc tgtcgggcgt gaccatgccg ccgccgtcat ctccacatgc
     2221 cgcgaccgcg atcagcgcca agacataacg cttcatcgcg ccacgataac tcaaagcttc
     2281 ttgaagtagt cgcccacgac ctcgaagtcc gcgatgaact cttccttcgc gatgcgccgc
     2341 atctcctcgt cgggcgcgcg taagacggac gcgggatgga cggtcgcgaa cgtccacttc
     2401 gcgagctgcg tgtcgagcgc ttggcctctc tgcttcgtca cgcgaaacgc cgagccgagc
     2461 agcgcctgcg cagcggtcgc gccgagcagg acgacgatct ccggctcgat cacttcgagc
     2521 tcgctgtcga gccacggctt gcacgcagcg atctcgtacc ggttcggctt gtcgtgcagc
     2581 ctgcgcttgc cccgctgcgt gaacttgaag tgcttcacgg cgttcgtcac gtagacctcg
     2641 tcgcgcggga tgccggcgag cgcgagcgct tcgtcgagga gcttgccgct tggaccgacg
     2701 aacggcttgc ctgcgagatc ctcgcggtcg cccgggactt cgccgaccag catcactttc
     2761 gacgacgcgc tcccttcacc gaacactgct tgcgtcgccg ctttgtacag cgggcatcca
     2821 cgacacgtcg ggaccgcagc gcgcagtgcc gtgaggttgt gcttgaccgg taagaatgat
     2881 tcggcgcccg gaacttcctc gggcgacggc tttggaagtc gcgcacgaac cgccatgcag
     2941 tgatgccaag cacgcgatgt gccgtgcgcg cgtggcacgt ctgatgctcc tccttagtgc
     3001 atgaagacga cacttggaat catcacttct ctgctgctgt cgtcggctgc attcgctggc
     3061 aacgcagcgc ctgatttcac cggagcagca ccgcgcccgc acatcgacaa gtcgctggtc
     3121 gcgtcgagcg gcaccgcgcc gatcgagccg ggtgacgtgc tgcagttccg cagcgacgaa
     3181 gcgatcctga cgacgagcgg ttactcgcaa gtcgatcgcg ccgcgctgtg gctgaaggtc
     3241 caccccaagg acaacatcgt catcgagggc caagcggatc acccgggcac cgagccatac
     3301 aactcggacc tctcgatgct gcgcgccgag gcggttcgca agcgcctcat gtcgcaaggc
     3361 atctcgcgca agcgcatcat catggtcgcg agcggcgaga aggatcagct gctcagcccg
     3421 gccgatcgcc gcgttcgtat gtatgcgacg aagctgtcgc cgcaggcgat ctacgcgatg
     3481 accgcggaac aagccaggaa tgcccctgcg gtcgagcaga cggcccgtcg ctgagggtgg
     3541 tatgctgccc ctcgcggggc catgaagaag tttcctgact tcgttccacc caccaaggtc
     3601 agctacctcg accgaggtcc gacgctgcat ctgcgtcgct gcatcttgca gacggaggac
     3661 gacagtcagg agtgggtctt cgacaaagaa gagatccgca tcggctcgat ggaagacaac
     3721 gacgtcgtcc tcgccgacga cacggtgtct cgctaccact gcaagatcat ccaggacgac
     3781 accggctacg tcctggtcga caaccattcg acgaacggca cgttcatcaa caaggtgcgc
     3841 gtccgcgagg gcttcctcaa gccgggctgc tcgctcgccg tcggccaatc gctcctcaag
     3901 ttcaacgcgc gcgaagaaga ggtccagatc gttccgtcgc gcgccacgcg ctgcggcaac
     3961 ctgatcggcg gcaacgcgaa gatgcgcgag atctactcga tcatcgagaa gatcgcgccg
     4021 acggccacga cggtcgtcat cgatggcgag accgggaccg gcaaagaggt cgtcgcgcag
     4081 tcgatccact cgctgtcgcc gcgcagcaag aacgagctcg tcgtgttcga ctgcggcgcg
     4141 gttccgccga acctgatcga gagcgagctg ttcggccacg agaagggcag cttcaccggc
     4201 gcgatgatga cgcgtaccgg cctgttcgag caggctgatg gaggcacgct gttcctcgac
     4261 gagctcggcg cgctgccgct cgacctccag ccgaagctgt tgcgcgcgct cgagcagcgc
     4321 gaagtccgcc gcgtcggtgg cgcgaagtcg aacaaggtcg acgtccgcat catcgcagcg
     4381 acgaaccgca acctcgagga cgaagtccgc gcaggtcggt tccgccagga cttgttctat
     4441 cgcctcagcg tcgtgcgcct gcatctgccg tcgctgcgcg atcgcgccga tgacatcccg
     4501 gtgctcgtgc agcacttcgt cgagacgggg ccgtataacc gcgggcccga cggcaagccg
     4561 cgcgtgcgtg gcgtctcgcg cgaagcggtc aacgcgctgc aggcatatcc ctggccaggc
     4621 aatgttcgcg agctcgtcaa cgtgatcgag cgcgcggtct cgttctgcac gcacggccag
     4681 atcgagctgt cggatcttcc tgactacgtg cgaacggcga agcccgcgcc gcagaaagag
     4741 catcaaggcg cgcgcccgct gccgcggcag ccgaccggcg gcaatcccac cgtcggcagc
     4801 gcgctctcgc cgacgccgcc cgcgacgccc gacgagctgc tcgccgaagg cgtcacgttc
     4861 aaggatgcga aggagcgctg ggtcgcgacg ttcgagcgcg actacatctt gcagatgctt
     4921 cgtcgcaaca ccggcaacat ctcgcacgct gcgcgctcgg cagacatcga tcgcaagtac
     4981 ttccgcaagc tgatgaagaa gtacgacatc gaggccgcgg gcgtcgatga tgacgacgcc
     5041 gtcgagtgaa gaagacgacg ccgacgcgct cctcgacgac gaagaggatg cagcgcccgt
     5101 cgacgatgga gccggcgctc acttagacga gaacggcaag ccgcgcatca tcgagcgcca
     5161 cctgatcgtc acgccggagc tcgcgggcct tcgcctcgat cattacgtca agacgcagat
     5221 ccaacggctg tcgcgcacga agatccaagc gatcatcgag acgcagctgc ggcgagcgga
     5281 cggtagtgcc gtcaaatcgt cgacgacggt cgcactcggc gaccacttca tcattcgccg
     5341 gcaagcgcgc gcagagccgc cctgccctcg cacgttcacg gtgctctacg aagacgcgcg
     5401 catgcggatc gtcgacaagc ccgcgatgct gcccgtgcac gcgacggcga agttctactt
     5461 caacacgctg acgcgcgtgc tctgggagcg ctatcccgac gaggacgagc tgcagctctg
     5521 ccatcggctc gatcgcgaga cgagtggctg cgtcgtgctc gcacgcgaca aggcagctgc
     5581 ggcaaagctc aaaggcgcga tcgccgccaa agacaagacg acgaagcaat acgtcgcggt
     5641 cgtgcacggc acgccgccct ggaccgaaga gacgacgatc gacctgccgc ttcgcctcgc
     5701 cggccccgcg gacgcgacgc gcctacccca cgtccgcatg ctccccgcga cgatggccga
     5761 gcccgatgcc cagtcggcga tcacgcgcgt cgtcgtcgag cgcgccggca atccctactc
     5821 gctcgtgcgc tgcacgctcg ttaccgggcg ccagcatcag atccgcgcgc acctcgcggc
     5881 ccgcggcttc ccgatcgtcg gcgacaagat ctacacgcac ggcgatgacg cgttcatcga
     5941 gtactgcgcg agcggcctca caccggagct cgctgcgcaa ttcgtgcttc cgcgccatgc
     6001 gctccatgct gcgcgcatca ccgtcccgca tcctgaagat gggcgcccga tcaccgcgat
     6061 cgcgccactg ccctccgaca tggcgacgct cctcggggag tagtcccacc aatgtggaac
     6121 cgagcgtcag cggccactcg aaaagccact gctgtacgcg ttgagtgcgc cctcacaatg
     6181 cccgccggga aacgtgtccg gcgcctgtct cctcgggtcg tcagtgtcac gtggcgcagt
     6241 cgctgcagtg tcccgagagc atgaaggact tccagacgtt gaccaccgag gagctcacca
     6301 ccgcacacgg aggcatgagc gccgcagcac gcttcatcat gatgcacgag tcgggcggca
     6361 gcacgaccgc tggccacctg tacaagcagg gtcgcggcga cggcacgcgc ggcaaccact
     6421 cgtcggcatt cggcgccttc cagatgatcc aagcgacgcg caaggcgtac atgggccgcg
     6481 actaccagtc gacggacttc aacaagcagt acgccgccgc gtcgcactac gtccgcgatc
     6541 gctacggctc gtgggagcgc gcggcctcgt tctggcgctc gcaccactgg tactaagcgc
     6601 gcgtaccgcc gaggaaactt cgtcgtggaa tcgggcggtg ggtgcctgta taggtgaggc
     6661 atgcgccgtt cggttgttgc ggtcttgttc ggcctcgtag cggcatgtgg caattcgagc
     6721 ggcacgaccg gcgatgccaa tggcggcgac gacgatcaaa cgggcgacga cgcgggcggt
     6781 acgtccggcc cccggaccgt caagctgacg ctgacgaacc ggccgatgac cgctgcgaat
     6841 ttctccttcc tcgtcgcgta tcaagatggc agcgctgcgt ggcagctcgc accggcaccg
     6901 gcgggcgacg tgtactcgtt cgagatcaac gcgccgaact acggcgtcgc gtttgcgtgc
     6961 atcggcacga cggcaggcac gacgacgcag gtgcggagcg tgacgtccgc gtacttcgcg
     7021 gtcggtgagc gcacggagct cacgctcgac gtaccggcgc ggtgcagcga tcgccagacg
     7081 acgatgggcg cggtcatgct caacggctcg gtgacgaatc ggccgttcaa cggcggaaca
     7141 ctcgtcgccg tgtggggagg ccgtagcgcg tttgtcggca cgcagaccgg aaacttccag
     7201 ctcacggtac aacccggcac gcacgatctg ttcgtcgtgc acgcggttcc cgagggcaac
     7261 ggtgatttct acaccgacga ggtgtgggtc gcgcgtgacg tcacgctgac ggcgaataca
     7321 aatcgcacga tcaacttcaa cgaagcgcag tcgacaacgc agtatgcagt caacatcggc
     7381 acggtcgacc ccgccgcgcg cgttacggct agcacgacgg tgtacaccgc gaacggaacc
     7441 gctgccggac tcgtgcgcga gacgatgaac tgggaaacgt cgtcgctcgc cgacgcgcag
     7501 atgaagacga ccgacgtcta cgatcagtcg atccaagtat cgagcttcgc gaagagcgcg
     7561 acggtcacgc acgcgacgaa cgagcccggc gacatcgact ggaccgctcc agcgccgctg
     7621 ggtgccgtgc agtcgatgac cgcgacgaag atgccgtacg tgatcctgca ggcgacgtgg
     7681 ccggcctatg ccgatgtcga tggttatctg tggaatgcgg cgcagcagct ctcgggatcg
     7741 cagtgcggcg gcaatacgag ctccgcgtgc tcgatcgtgt ggcaggcgta tctgtcgccc
     7801 ggcgtgacgg gtgcgatgcc agcgttccgc atgcccgagc tcgcagacct cgccggctgg
     7861 aaggatgcgt tcgagctggt ctcgggcacg caggctttcg gcagcgtgac ggcgtacacg
     7921 tcgagcgccg gtgcaggaga tttcccggcg ggtgtccccg caaatggcac gacgcgcacg
     7981 ttcgttcgaa gcgacttcgc gacgacgccc tgacgcgtgc tagaccaaca aggctgtggc
     8041 caagaaggac cgcgccgcgc cgttttcgaa gaagcaccgc cggtattgga tcccggtgac
     8101 gggcggcatg ctgctgatcg gcgccatcaa cgtggcgctg ggcatgtgca gctacaaaga
     8161 ggcgccgccg accgagcgca tggagctggt gattccgggc cacgagcgca ggcccgggca
     8221 gctgatgcgc gcagaaatcc ccgctgacgt aatgcgcgcg tttgcagtga agtacccgca
     8281 cacgattccc gacggtgcga tcaccaacgg tacggacaac acgatcatcg tgcagttccc
     8341 gccgggcaac gcgctgcaca cggcggtgtt caagctcgac ggcacgttcg tcagcgaaca
     8401 gtagctacgg cgcgaactgt aggtcgtacg gcaagcgcgc ctgcgcggca tcgggcgaca
     8461 cgagcagcga tgcgggcaag cccgacagca gcgagcgcac gatcggcagc tcgaataccg
     8521 agctctgctc cttcacaccg agcaagctgc cgagcggacc gaaaatgctc gacggcaact
     8581 tcggcagctc gtaaatggtg acgcgcgtcg atgggtcgag gcccatgcgc ttcttcgcgt
     8641 cgtcgagcgc atcgccgagc ccgccgaact tgtcgacgag ctggatcggc ttggccatat
     8701 cgccggtgta gacgtgaccg cggccgaggc cgtcgacctg ctccttcgtg agcttgcgtc
     8761 cttcggagac cgcgccgacg aagcggctgt acatgtactt gagcttctcg agcagtgccg
     8821 tgcgttcttc gtcggtgtac ggccggaaca tgctctcgac atcggagcgc ttgccgcgct
     8881 tgtacgtgtc ggtcgtgacg ccgaccttcg ccatgaggcc gctcagatcg aacttgccgt
     8941 agaagatgcc gatcgatccg gtgatcgtca tcggctcggc atagatcgtg tcgcagccgg
     9001 cagcgatgaa gtacccgccg gatgcggcga gatcgctgaa cgagcagatc accggcttga
     9061 cgccgcgcgt ctggaaaatc tcgcgcgaca ccagctcgga cgcgagcgcg ctgccaccgg
     9121 gagaatcgat ccgcatcacg atcgcgccga cgcgaggatc gttgcgcgcc gccgtcaccg
     9181 ctgcgacgag cgtctcgccg cctgccacgc tgcgcccgag gatcggaatc gtctgcgact
     9241 tgccgtccgt gatgtctccc tcgacgtaga tgacggcgat gccagggcgc gtccagcggt
     9301 cggggcgatc cgcgccgggc gacgtcacgg gatacgagcg accgatctcg gtgacgatca
     9361 tctgcgcgac tttgtcggga gatgcgaccc cgtcgacgag cttctggtcc ttcgccagat
     9421 cgcctgctgt gtacggcccc gcatcgacga gcgccttcac ctcgtccttg ctcaggtggc
     9481 ggcccgcggc gatcgccgtg agccactgat cccagagcga gtcatagagg tcgttcgcca
     9541 tcttcgcggc cgtgtcggtc gctttcttct cggtgaactg ctcgggcgcg ctcttgtact
     9601 ccgcgatctt ctcgaactgc ggcgtcacgc cgatcatgtc gaacgccccg cgaaaatgca
     9661 gcgtctctcc cgccatgccg acgagccgca cgccaccggc cggatcgaca tagattttgt
     9721 cggccgcggt cgcgacgaag taatcgcgcc ccgttccgga caccatgtac gcgaacactt
     9781 tcttgccggc cttcttcacg gcgagcagct cgtcgcgcag ctcctgcagc gtggcccatc
     9841 cgccgtccgc gccgtcgaac atgacgatga cggctttcgc cgtcgagtcg tgcgcgatcg
     9901 accgcagtcg cagcacgatc gatgtcagct cgcgcgagcc aatggtcccc gacagctcga
     9961 cgcgctcgat gtgatcgcca tcgcccaaga ccgatgcgg
//
LOCUS       DATLBZ010000454         5607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4278069, whole genome shotgun sequence.
ACCESSION   DATLBZ010000454 DATLBZ010000000
VERSION     DATLBZ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4278069"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VMZ53_22930"
     CDS             complement(<1..941)
                     /locus_tag="VMZ53_22930"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HUS31392.1"
                     /translation="MRTITLVSILAAAAGGCTMAGAKHAYVASPTSMDMAMAPPPGYP
                     TAEAQPAANSESYTDYGKNPWVMTDKDRFSTFAADVDTASFTIGRRKLMQENALPPQA
                     SVRVEEWVNYFKYSFPPPHAGTPFSTVMEAAPHPFAQDRYVLRVGVATKAKTLGERKP
                     SALVFLVDVSGSMSSDDKLGLAKRALHLLTENLTEKDAVSIVTYAGDSRVVLPMTSTD
                     TKDGKSRIHKAIDSLGAGGSTAMAAGIDSAYEQAAKWIRPGAISRVIVLSDGDANVGS
                     HTHEEMLRIIEDRAKQGVSLSTIGFGMGNYKDTTMEQL"
     gene            995..1540
                     /locus_tag="VMZ53_22935"
     CDS             995..1540
                     /locus_tag="VMZ53_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31393.1"
                     /translation="MRSALLAIVLILVGCDAGERTPTGPRPDQLALREIVEYTARAQR
                     EIDSVAKAVLERYAADHDSVPDSGLLQHETTILVDRDLAYDGTGGTLTADALPRGAFA
                     LRSPSEMQTDADRTDKYVHYIFIHDLHIEGDRATFWVGVHLKLPNHPEGPGTCCCSAE
                     QLYVKRNGAWTYAETTSSICS"
     gene            1578..4460
                     /locus_tag="VMZ53_22940"
     CDS             1578..4460
                     /locus_tag="VMZ53_22940"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31394.1"
                     /translation="MPRDLDPLAETQVNTPSDAGEAKSDPALDQTAADSLVGRASFSV
                     GEQLKIGRFLALEKLGEGGMGVVIAAYDPQLDRKVAIKMMHDHVSELQGSRLAREAKA
                     MAQLNHPNVLTVHDTGLYGDRLYIAMELVDGTTLAKWLRAKPRSQAEIFDVIMQAGRG
                     LAAAHAVGLVHRDFKPDNVLVGNDGRVRVSDFGLVSSSGEAPTNDGKKPKVDAHAALT
                     QTGAVMGTPLYMAPEQHAGKVADAKADQFSFAVTLWQALCDEMPYGADSYEALVANVT
                     GGKLRAIPRGVRVPAKIKEILARGLESDPAKRFGSMKELLAALDRARRPNRQPLIIAG
                     AVVAGLAIGGGVLLMGGRHQAATEDVCGGGTKRVSAVWDDKRRASLASAFSAIGGDDV
                     WKQLDTIVSRYAQTWALHHRDICSASLVRHEQSSELYDLRMRCMDGRLADFDALLTRI
                     ATTKKETRFKTIDAVAALPDFTDCDDSERLRAAIPLPNDAAVRAKIADIESGFTEAVS
                     YDRTGDAKTAKSILDPLVPKAKELGYAPLDAKLVLLRAGLELRAGDLETAAASYRESA
                     EVAARARDDVRIAQSWIDLMNVLGLQGKYADALALEPIARTSTERVSDEPRLVARFAN
                     TLAGIYLAQGKYPDARREYEKALEAVRKDRPDSDLLAPALGNMALALWYTGDMPGAQK
                     FYADARDRAIENVGPKHPTLAYLYRNIGDLAAATNDIDTALENYKKSLAIFEGTNGPD
                     HIDVAIALEPLSYAYARKGDVAKSREAGLRALKLREAKYGPEHPTIANTLNSLADAEV
                     MDGRPESLTLAIAYLDRSIAIQEKAFGTQHPKLPDSLDRLGNAQLKAKKYADAVTTFQ
                     RSLAIKRKTMGPTANDVALVETELGQAALMVKKYSVASDAYIAAEGIFRGNNPKDPNI
                     AIALLGRANVEAAQGHRAKAVELAKQARELAEEPVRGMVDAFLAGK"
     gene            4529..5584
                     /gene="pstS"
                     /locus_tag="VMZ53_22945"
     CDS             4529..5584
                     /gene="pstS"
                     /locus_tag="VMZ53_22945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HUS31395.1"
                     /translation="MKRIALIALTLLACSKGEKTNDQPAGSGGTKPSAGNATLNASGS
                     TFQKAFQEEAIAELTTKNPGVKINYGGGGSGKGRQDLSDMVTDFAGSDSPFKDADLAK
                     VKGGEVLYFPVVLGAVTVSYNAGVDKLQLSPDTIAKIFQREVKTWNDPAIAADNPGVT
                     LPATAIVVVHRADGSGTTDIFTKFVEGASTAWKLKSGSTVEWPADTQAGQGNGGVAQL
                     VKSTAGAIGYVDLSDAKASGLKYASIKNSAGKYVEPNAASVSAAAEAVDVKDNLTFSA
                     VNGKGDAAYPIAGQSYIIVYAKQSDAAKANAIKAYLTYLLGDGQKLLEPLDFATLPKA
                     LADKALAQLSKIQSPGA"
BASE COUNT         1062 a         1798 c         1834 g          913 t
ORIGIN      
        1 agctgctcca tcgtcgtgtc tttgtagttg cccatgccaa agccgatcgt cgacagcgag
       61 acgccttgct tcgcgcggtc ctcgatgatg cgcagcatct cttcgtgcgt gtgcgagccg
      121 acgttcgcgt cgccgtcgga gaggacgatc acgcgcgaga tcgcgccggg gcggatccac
      181 ttcgcggcct gctcgtaggc gctgtcgatg cccgctgcca tcgcggtcga gccgcctgcg
      241 ccgagcgagt cgatcgcctt gtggatgcgc gacttgccgt ccttggtgtc ggtcgacgtc
      301 atcggcagta cgacgcgcga atcacccgcg tacgtgacga tcgagaccgc gtccttctcg
      361 gtcaggttct ccgtgaggag atgcagcgcg cgctttgcaa gcccgagctt gtcatcactc
      421 gacatcgatc ccgatacgtc gacgaggaac acgagcgcgc ttggcttgcg ctcgcctagc
      481 gtctttgcct ttgtcgcaac gccgacgcgc aacacgtagc gatcctgcgc gaacggatgg
      541 ggcgccgctt ccatcaccgt cgagaacggc gtgcctgcat gcggcggcgg gaagctgtac
      601 ttgaagtagt tcacccactc ctcgacgcgc acgctcgctt gcggcggcag cgcattctct
      661 tgcatgagct tgcggcggcc gatcgtgaac gatgccgtgt cgacatcggc tgcgaacgtc
      721 gagaaccgat ccttgtcggt catcacccac gggttcttgc cgtagtcggt gtagctctcg
      781 ctgttggctg ctggttgcgc ctcggccgtc ggataaccag gaggtggcgc catcgccatg
      841 tccatcgagg tcggcgacgc gacatacgcg tgcttcgcac cagccatcgt gcaaccaccg
      901 gctgcggcgg cgagaatcga tacgagagtg atagtgcgca tttgctgaaa caacgcgcgc
      961 aacccgcgac ggcttacacg gttcctaaag acacatgcgt tctgcccttc tagcgatcgt
     1021 cctgatcctc gtgggatgcg acgcaggtga gcggacacct acagggccgc gccccgatca
     1081 gctcgcgctc cgcgaaatcg tggaatacac ggcgcgcgcg cagcgcgaga tcgactccgt
     1141 cgcaaaggca gtactggaac gctacgcagc ggatcacgac tccgtacccg acagcggctt
     1201 gcttcagcac gagacgacga tcctcgtgga tcgcgatctc gcgtacgacg gcacgggcgg
     1261 gactctaacg gccgatgcat taccgcgcgg agccttcgcg ctgcgttccc cgagcgagat
     1321 gcagaccgat gccgaccgca cggacaaata tgtccactac atcttcatcc acgacctgca
     1381 catcgaaggc gatcgcgcca cgttctgggt cggcgtccac ctgaagctgc cgaatcatcc
     1441 cgagggacct ggcacgtgct gctgctctgc ggagcagctc tacgtgaagc gcaacggcgc
     1501 atggacgtac gccgagacga cgtcttccat ctgcagctag ctgcgttgtc gcgacgcgcg
     1561 actgatacac tgggctcgtg ccgcgtgacc tcgatccgtt agcggagaca caggtcaaca
     1621 cgccgtccga tgcgggcgag gccaagagcg atcccgcgct cgatcagacg gcggcggaca
     1681 gcttggttgg acgcgcgtca ttctccgtcg gcgagcagct gaagatcggc cgcttcctcg
     1741 cgctcgagaa gctcggcgaa ggcggcatgg gtgttgtcat cgctgcgtac gacccgcagc
     1801 tcgatcgcaa ggtcgcgatc aagatgatgc acgaccacgt cagcgagctg cagggctcgc
     1861 gcctcgctcg cgaggccaag gcgatggcgc agctcaatca cccgaacgtg ctgacggtgc
     1921 acgacacggg gctctacggc gatcgcctct acatcgcgat ggagctcgtc gacggcacga
     1981 cgctcgcgaa gtggttgcgc gcgaaaccgc ggtcgcaggc tgagatcttc gacgtgatca
     2041 tgcaagccgg gcgtgggctc gcagcagcgc acgcggtcgg gctcgtgcac cgcgacttca
     2101 agcccgacaa cgtgctcgtc ggcaatgacg gccgcgttcg cgtctccgac ttcgggctcg
     2161 tgtcgtcctc gggggaggcg cccacgaacg atggaaagaa gccgaaggtc gacgcgcatg
     2221 ccgcgctcac ccagaccggc gcggtgatgg gcactccgtt gtacatggcg cccgagcagc
     2281 acgcaggcaa ggtcgccgat gcgaaggcgg atcaattcag cttcgcggtc acgctatggc
     2341 aagcgctgtg cgacgagatg ccgtacggcg ctgacagcta cgaggctctc gtcgcgaacg
     2401 tgacgggcgg caagctgcgg gcgattccac gcggcgtacg cgtgccggcg aagatcaaag
     2461 agatcctcgc gcgcggtctc gagagtgatc cggcaaagcg gtttgggtcg atgaaggagc
     2521 tgctcgctgc gctcgatcgt gcgcgacgcc ccaatcgcca gccgctcatc attgcggggg
     2581 cggtcgtggc cggccttgcc atcggtggag gtgtcttgct gatgggtggt cgccatcagg
     2641 cggcaacgga ggacgtgtgc ggcggcggta ccaagcgcgt ctccgcggtg tgggacgaca
     2701 agcgacgcgc gagcctggcg tcggcgttta gcgcgatcgg tggcgatgat gtgtggaagc
     2761 agctcgacac gatcgttagc cgctacgcgc agacgtgggc gcttcaccat cgcgacatct
     2821 gcagcgcgag cctcgtgcgc cacgagcaat cgtccgagct ctatgacttg cgaatgcgct
     2881 gcatggacgg gcggctcgcc gatttcgacg cgctgctcac gcgcatcgcc acgacgaaga
     2941 aggagacgcg gttcaagacg atcgatgcgg ttgctgcgct tccggacttc accgattgcg
     3001 acgacagcga acgtctgcgc gcggccatcc cgctgccaaa cgatgccgcc gttcgcgcga
     3061 agatcgcgga tatcgagagc gggttcaccg aggccgtctc gtatgaccgc accggcgacg
     3121 caaagacagc gaagtcgatc ctcgacccgc tggtcccgaa ggcgaaggag ctcggctatg
     3181 cgccgctcga cgcaaagctg gtgctgttgc gcgctggtct cgagctgaga gctggcgatc
     3241 tggagacggc cgccgcgtcg tatcgcgagt ccgcggaagt cgccgctcgc gcccgtgatg
     3301 acgtccgcat tgcacagagc tggatcgatc tgatgaacgt gctcggcttg cagggcaaat
     3361 acgccgatgc gctcgcgctc gagccgatcg cgcggacgtc gacggagcgc gtctccgacg
     3421 agcctcgcct ggtcgcacgg ttcgcaaaca cgctcgccgg catttatctc gcgcaaggca
     3481 agtatcccga cgcgcgtcgc gagtacgaga aggcgctcga agcggtgcgc aaggatcggc
     3541 cggacagcga tttgctcgcg cccgcactcg gcaacatggc gctcgcgctc tggtacacgg
     3601 gcgatatgcc gggcgcgcag aagttctacg cggacgcgcg cgatcgggcg atcgagaacg
     3661 tcggaccgaa gcatccgacg ctcgcgtacc tctatcgcaa catcggcgac ctcgcggccg
     3721 cgacgaacga catcgacacc gcactcgaga attacaagaa gtcactcgcc atcttcgagg
     3781 gcacgaacgg tcccgatcat atcgacgtcg cgatcgccct cgagccgctg tcgtatgcgt
     3841 acgcgcgcaa gggtgatgtc gcgaagtcgc gtgaagcagg cctgcgcgcg ttgaagctgc
     3901 gcgaagcgaa gtatgggccc gagcatccga cgatcgccaa cacgctcaac agcctcgcag
     3961 atgccgaagt gatggacgga aggcccgaat cgctgacgct cgccatcgcg tatctcgatc
     4021 gatcgatcgc gatccaggag aaggcgttcg ggacgcagca tccgaagctt cccgactcgc
     4081 tggaccgtct cggcaatgcg cagctcaagg caaagaagta cgcggatgcc gtcacgacgt
     4141 ttcagcgcag cttggcgatc aagcgcaaga cgatgggccc gaccgcgaac gacgtcgcgc
     4201 tcgtcgagac cgagcttggc caagcggcac tgatggtgaa gaagtactcg gtcgcaagcg
     4261 acgcgtacat tgccgccgag gggatcttcc gcggcaacaa tcccaaggat ccgaacattg
     4321 ccatagccct cctcggacgc gcgaacgtcg aggctgcgca aggtcatcgc gcgaaggcgg
     4381 tcgagctggc aaaacaggca cgggagctag cggaagaacc ggtccggggc atggtcgacg
     4441 cgtttcttgc cggcaagtag tcgatctcgt caccgtcccg tcacgtgcgt gccgcgaggt
     4501 tgccgcatcc gcttcgtagc ttgcgtgcat gaagcggatc gcgctgattg cactgaccct
     4561 cctcgcctgc agcaagggcg aaaagaccaa cgaccaaccc gcgggcagcg gcggcacgaa
     4621 gccgagcgcc ggcaacgcca cgctgaatgc cagcggctcg accttccaga aggcgttcca
     4681 agaagaggcg attgccgagc tcacgacgaa gaacccgggc gtgaagatca actacggcgg
     4741 cggtggttcc ggcaaaggtc gccaggacct ctcggacatg gtcaccgact ttgcggggtc
     4801 ggactcgccg ttcaaggatg cagacctcgc gaaggtgaag ggcggcgagg tcctctactt
     4861 cccggtcgtg ctaggcgcgg tgaccgtcag ctacaacgcc ggcgtcgaca agctgcagct
     4921 ctcgcccgac accatcgcga aaatcttcca gcgcgaggtc aagacctgga acgacccggc
     4981 gatcgccgcg gataacccgg gcgtgacgct gccggcgacc gcgatcgtcg tcgtgcatcg
     5041 cgccgatggc tccggtacga cggacatctt cacgaagttc gtcgagggtg cgtcgaccgc
     5101 gtggaagctg aagagcggct ccaccgtcga gtggcccgcg gacacgcaag cggggcaggg
     5161 caacggcggc gtcgcgcagc tcgtgaagtc caccgcaggc gcgatcggtt atgtcgatct
     5221 ctccgacgcg aaggcgtccg gtctgaagta cgcgagcatc aagaacagcg ccggcaaata
     5281 cgtcgaaccg aacgcagcgt cggtgtccgc ggccgcagaa gcggtagacg tgaaggacaa
     5341 cctgacgttc tccgcggtca acggcaaagg cgacgctgcc tatccgatcg cgggacagag
     5401 ttacatcatc gtctacgcga agcagtccga cgccgcgaag gcgaacgcga tcaaggcgta
     5461 tctgacctac ctgctcggcg acggccagaa gctgctcgag ccgctcgact tcgcgacact
     5521 gcccaaagcg ctcgcggaca aggcactcgc gcagctctcg aagatccaat cgccgggagc
     5581 ctagcgatgg acctgcgcgg caagaac
//
LOCUS       DATLBZ010000455         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6246831, whole genome shotgun sequence.
ACCESSION   DATLBZ010000455 DATLBZ010000000
VERSION     DATLBZ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6246831"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VMZ53_22950"
     CDS             complement(<1..672)
                     /locus_tag="VMZ53_22950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31396.1"
                     /translation="MPVIARDAVASDHLVYAWPFPIGPEEYEVSCAEDELVVMCPSWL
                     VGDRLGPGRHRWRTPDPSRPVSAYFVLTAPVEVGFDVLTQFVIPTTGQPVRLRAIGSL
                     QVRCMDPALLVAQFVGLPFDHVNEGVLRSVTRSIERMLARLLTRRVVMASTPHAVTDP
                     SMHPHIIEELVAYNPAAGAVFGVELVRFGHMSIIADDGSRPWHEMVPTPQARHGGPNG
                     ETERRV"
     gene            complement(745..2058)
                     /locus_tag="VMZ53_22955"
     CDS             complement(745..2058)
                     /locus_tag="VMZ53_22955"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HUS31397.1"
                     /translation="MGISIVIVLAFLVGNGLFAGAEIAILSVRTTRLREFIRRRDKRA
                     LAVKKLRDQPERFLATVQVCITTFGTAASVYGGATLEPYFEHQLKSVGLGHLTSLIAV
                     VIMIVFFELIIGELVPKSLALRASDRYSFFTARPLLWIAQLLRPVVWSLTAASNLVLR
                     FFGDKTTFTETRLSKDELQQLVEEAAKTGSLDPRASEIASRALGFGEVTVAEVMVPRN
                     RVVALRRGSPPEELQRVILEEGHSRMPVYDTSIDNIVGYVVARDVLALAWEKGLLVLE
                     DILRPPHKVPESTRALDLLRELQRRRTQMAVVVDENGGLSGLVTAEDLIEELVGDIMS
                     EDDVPEQYFVREPSGTILVQGWAAVRKVNRDLHLDLPVGKDRTTIAGLCMSLAQAIPQ
                     AGEKLTTDNGTVLEIIEASPRRVRRVRIHPVADADSEEPSEPASE"
BASE COUNT          388 a          705 c          712 g          324 t
ORIGIN      
        1 cacgcggcgc tcggtctcgc cgttcgggcc tccgtggcgc gcttgtggcg tcggcaccat
       61 ctcgtgccac ggacgcgagc cgtcgtcggc gatgatcgac atgtgaccga agcgcacgag
      121 ctcgacaccg aagaccgcgc cggcagccgg gttgtatgcg acgagctcct cgatgatgtg
      181 cggatgcatc gacggatccg tcaccgcgtg tggcgtcgac gccatcacca cgcgacgcgt
      241 gagcagacgc gcgagcatgc gctcgatgct gcgagtcacg ctgcgcagta cgccttcgtt
      301 cacgtgatcg aacggaaggc cgacgaactg cgcgacgagc agcgctggat ccatgcagcg
      361 cacttgcagc gaaccgatcg cgcgaaggcg caccggttgg cccgtcgtcg gaatcacgaa
      421 ctgcgtgagc acgtcgaagc cgacctcgac gggagccgtc agcacgaagt acgcagacac
      481 cggccgcgat ggatccggcg tgcgccaacg atgccggcca ggaccgagcc tgtcgccgac
      541 gagccacgat ggacacatca cgacgagctc gtcttccgcg cacgacacct cgtactcctc
      601 cgggccgatt ggaaacggcc aggcgtatac gagatggtcc gacgcgacgg cgtcgcgcgc
      661 gatcacgggc atcaacgcga tactacagga tctcgacggg ggaaggtcgc gacgaggagc
      721 gtcgtaaaat gcacaacccg cgcactactc gctggccggt tcggacggtt cttccgagtc
      781 ggcgtcagct acgggatgga tccgcacgcg acgcacgcgg cgcggtgatg cctcgatgat
      841 ttcgagcacc gtcccgttgt ccgtcgtgag cttttcgccc gcttgcggaa tcgcctgagc
      901 gagcgacatg cagaggccgg cgatggtcgt tcggtccttg ccgaccggca gatcgaggtg
      961 gaggtcgcgg ttcaccttgc ggacggccgc ccagccctgg acgaggatcg tgccgctcgg
     1021 ctcgcggacg aagtactgct cgggcacgtc gtcctcgctc atgatgtcgc cgacgagctc
     1081 ctcgatgagg tcttcggcgg tcacgagacc ggacaggccg ccgttctcgt cgacgacgac
     1141 cgccatctgc gtgcggcggc gctgcagctc gcgaagcaga tcgagcgcgc gcgtcgactc
     1201 gggcaccttg tgcggcggcc gcagaatgtc ttcgagcacg agcaggccct tctcccacgc
     1261 gagcgcgagg acgtcgcggg ctacgacata gccgacgatg ttgtcgatgc tcgtgtcgta
     1321 gaccggcatg cggctgtggc cctcctcgag gatcacgcgc tgcagctcct cgggcggcga
     1381 accacggcgt agcgcgacga cgcgattgcg cggcaccatc acctcggcga ccgtcacctc
     1441 gccaaagccc agcgcgcgcg atgcaatctc gctcgcacgt ggatcgagag agccggtctt
     1501 cgcggcctct tcgacgagct gctgcagctc gtctttcgac agacgcgtct cggtaaacgt
     1561 cgtcttgtca ccgaagaagc gcagcacgag gttcgacgcc gcggtgagcg accacacgac
     1621 ggggcgcagc agctgcgcga tccacagcag cggccgcgcg gtgaagaacg agtagcgatc
     1681 cgaggcacgc agcgcgagcg acttcggcac gagctcgccg atgatgagct cgaagaacac
     1741 gatcatgatg acgaccgcga tcagcgacgt caggtgaccg aggccgacgc tcttgagctg
     1801 atgctcgaaa taaggctcga gcgtggcacc gccgtacacg gacgctgccg ttccaaatgt
     1861 cgtgatgcag acctgcacgg tggcgaggaa ccgctcgggc tggtcgcgca gcttcttgac
     1921 ggccaacgcg cgcttgtcac gccggcggat gaactcgcgc aggcgggtcg tgcgcacgga
     1981 caagatcgcg atctcggccc cagcgaacaa cccgttgccg accaggaagg ccagcacgat
     2041 cacgatactg atgcccatgc gcgacgacgc ggagcatacg ctatatgctc cgtgcgatgt
     2101 ccacgcggac ccgcagcggg gatcgcggc
//
LOCUS       DATLBZ010000456        10287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9515156, whole genome shotgun sequence.
ACCESSION   DATLBZ010000456 DATLBZ010000000
VERSION     DATLBZ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10287
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9515156"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1140
                     /locus_tag="VMZ53_22960"
     CDS             31..1140
                     /locus_tag="VMZ53_22960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS31398.1"
                     /translation="MTDEKREINERVLTDASLRSERRKTDAELAKKMSIDDVATDVVI
                     EAREKADTLMGEARALADLKAADNLTARDAREIHEARARADAAVDEARRGADTVARDE
                     HNERLVALARLLATERQDTDLKLELERLHADAQVTTREDFMAIVSHDLRSLLGGIALT
                     AELLKTAESGGDPFVKVKDYAGRIQRFCARMNRLVADLMDVASIEAGKLSLVRKRREV
                     ALLLRDATEAFERTAASQGITLTTSCSAPGPIDVDHERIFQVLTNLVGNALKFTPAGG
                     RISIAIDRSEDLIRFTVSDTGEGIPAGILGKIFDRFVQVVENDRRGLGLGLFIAKSIV
                     EAHGGKLWVESTPGKGSTFYFTVAATREALASSSE"
     gene            complement(1089..1487)
                     /locus_tag="VMZ53_22965"
     CDS             complement(1089..1487)
                     /locus_tag="VMZ53_22965"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HUS31399.1"
                     /translation="MTRGAGSSYGFMAAFALVALWAGTGPLFGFSSAWQMIINTATTI
                     ITFLMVFVIQHSQNRDTLAIQLKLNELIASNASANNKLVDIEELSPEELAVVKKFYVK
                     LAAHTETAANVRASHSLDEASASRVAATVK"
     gene            complement(1554..2585)
                     /locus_tag="VMZ53_22970"
     CDS             complement(1554..2585)
                     /locus_tag="VMZ53_22970"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HUS31400.1"
                     /translation="MFAIVALSFTACGDQLVEFGRDAGSNSGSGDAVGSRPTVTVTSP
                     LDTTNNVSVNKRVTATFSRAMNGATLTSTTFTVHQGTTPIAGTVSYAGTTATFVASSP
                     LDVSLVYTARITTGAADPQGATLASDHVWTFTTGACSQAAVAMRGAGNYGVLAGSTVT
                     NTGPTSVTGDLGLSPGTAVTGFPPGTLVGTMHAGDSTSATANADLTTAYNDAAGRTLC
                     PVTVAGNLGGMTLAPGLYKSTSSLEISSGDLTLDAQGDDDAVFIFQMASTLTTTSGRQ
                     VILAGGANARNVYWQVGSSATLGTTSAFRGTIMADQAITLENGATLNGRALARIAGVT
                     LANNTIVKP"
     gene            complement(2609..3562)
                     /locus_tag="VMZ53_22975"
     CDS             complement(2609..3562)
                     /locus_tag="VMZ53_22975"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS31401.1"
                     /translation="MKLLFGVSRYFAFGPSVAFTTLPAEGTGDAGTSWAFGATGRLER
                     PHDAPGGRLSAISPWVDADLNYVRTGPLNRPGFAAAVGVALPLDENRKLWFGPFARYS
                     QIVQGDRAGFDNRDAKILSIGLSLEVGPGLMHRRRSPVTAQEEVTTPVADVTPPPPSP
                     SDRDGDTVVDDDDNCPDVAGIVSNAGCPVYAKVIVKRDKLELKEKIAFKWDSANLEDV
                     SFPLLDEVAQALKDNPGFKVQVEGHASSDGNYDHNQTLSERRAQAVLDYLIAHGVAAD
                     RVISKGFGSDNPTGTNSTVAGRESNRRVEFIVSFIIVEKAK"
     gene            3961..5025
                     /locus_tag="VMZ53_22980"
     CDS             3961..5025
                     /locus_tag="VMZ53_22980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS31402.1"
                     /translation="MHDSRAIGSNGFPELARAIVAAGHDVSKIMDALATSLTRTLCDR
                     CSIDRISSGEAEPAAPRALSPRHAILSLQGTKHLIGSVTVMRDDASSPFDGCDFADIE
                     TCITYASLAAEVALHLDAERRLLQAEHERAALFHRNMLGIVGHDLRAPVSAILLGTEM
                     IVAQHQDDPSIAVIATRIVSFANRITGMVDQLLDLSRAQLGGGIPLARCATRLGPVLE
                     SVVAELAARYPRNQFRLIGDVDVKGVWDPDRLRQVVASLLTNAVRHGLEDGPINIIIS
                     RVGNETSIAIHNELLGEPIPQEELRGLFEAYRQGEGEHHGPGLGLGLYIVREIVEAHR
                     GTIAVESTTSGTTFRIVLPE"
     gene            complement(5030..6445)
                     /locus_tag="VMZ53_22985"
     CDS             complement(5030..6445)
                     /locus_tag="VMZ53_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS31403.1"
                     /translation="MTCILVVDHDREFAELVRDGLRDRKYSAEAVRTAKEAIAFLDRN
                     PVDIVVAHLHLRDVSGLELVSRLRDRHLETLGIVVTSQGSLETAVQAIRAGAYDFICK
                     PVTLSVLEVAIERAVNYQALRREVQRLRLTVAASQPVDAILGDSAAIREVTSMIRRVA
                     ESPSTVLVTGESGTGKELVARAIHDLSDRKHQPFVAINCGALPPALLESELFGHVRGA
                     FTDARQNRHGLFVQAGEGTLFLDEIGEMPLEMQVKLLRVLQERKLRPVGGDAEVTFGA
                     RLIAATNRDLRTAIDEQRFRQDLYHRINVVEIKVPPLRSRPGDVRLLAQRFIEQLSAR
                     SRKPAPKLEDDAVRKLIAYDWPGNVRELQNCIERVIALLESSSISARDLPEAIRDYVP
                     SRTRTATGASDELVTLEELERRYIQQVLEVTRNNKSEAAKILGIDRRSLYRRLHDDDA
                     SPATTTDASTATTTDAPSQNN"
     gene            6654..7976
                     /locus_tag="VMZ53_22990"
     CDS             6654..7976
                     /locus_tag="VMZ53_22990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31404.1"
                     /translation="MTAPPDIALDPFDGHYELGELLGSGGMGNVYSAMQVSLGRRVAI
                     KLPQRHLVSNPLVLRRFRAEALAGSRIDHRNVARVMDFGERNGVLFLVMEYLPGVTLD
                     SLLLEHGPMLGSAGPDLCGQLLDGLAAAHSAGVIHADIKSANLIVEMRADGPIAHVID
                     FGLARFVNAPELEVERLLSGTPEYLAPELITGGLPSVASDLYAAGIVLYELLTGATPF
                     AGGTSDEILRRHSEDAVVPPSLRSPDQGIHAALDAVIIRALAKEPAARFETAASFAAA
                     LRACPRTLAVAPRAKRETAPPFSTESPTRDLHPEPPEDVASDTDRIALIRAAITEAFA
                     RNDGDLLVTSYLELARALIDDHDLPRAIAELERGLRMLRSTARETASGAWRLQLCLAA
                     LYSGTAQPERARNAATLGREDAARAASELGKTRAESLLARLKRSSART"
     gene            8028..8318
                     /locus_tag="VMZ53_22995"
     CDS             8028..8318
                     /locus_tag="VMZ53_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31405.1"
                     /translation="MNRAFSSRMDDDNDARHENGVDATRGQPILDRVLAHKVVLETKL
                     QTLSDDDRTTRADIELALATVRELLTGRLTHLPRVVAVDLNRWLERSKHLGG"
     gene            complement(8328..9068)
                     /locus_tag="VMZ53_23000"
     CDS             complement(8328..9068)
                     /locus_tag="VMZ53_23000"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HUS31406.1"
                     /translation="MKIVVLGGTGSLGRSIVEKATAAGHDVVAASRSGAVKVDVTTGQ
                     GLAEAFAGVDVIMDATNAQAGAHDVLVGGTKRVLEAAAAAGVRHFVGISIVGIDDAPL
                     AYYRTKVAQEKVIEASAVPWTLLRATQFHDLIPKLAAPKLGIVFAPRGFKIQPIDVRE
                     VAAVLVDAVAAGPQKRIPDVGGPEVRDFGDLARRWKRAAHKKRLVVRMPVPGATGAFL
                     RAGKLCCPDRAIGKTTFESWLAETYGTR"
     gene            complement(9065..9460)
                     /locus_tag="VMZ53_23005"
     CDS             complement(9065..9460)
                     /locus_tag="VMZ53_23005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31407.1"
                     /translation="MKKMLFFGALAVVGCGGGQKQTASPPTSTPPLANSAPAEATPPT
                     PKTETELAMDKMRGFRDQFCMCKDSDCARRVSDDMTAWAQDMAKKSDEPPKMTDAQTK
                     EMQQIGTQMGDCMVKAMQGSAPPPTGNNP"
     gene            9484..>10287
                     /gene="lipA"
                     /locus_tag="VMZ53_23010"
     CDS             9484..>10287
                     /gene="lipA"
                     /locus_tag="VMZ53_23010"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HUS31408.1"
                     /translation="MSERVHLPVVQAEDGAAAPAAPTQVVRRRHPEWIRAQLPTKPAY
                     FELRAMVKELKLHTVCESASCPNIGECWTRKALTIMIMGDICTRSCRFCDVTTGRPLP
                     LDPDEPRRVAEMLGRLDLEHTVITCVDRDDLADFGAAHWAETIRRVKDACPEMTLEIL
                     AGDFKGKPELVDIVLDADPDVFAHNLETVPRLSRQVRVQASYPRSYKILEHARKRGAV
                     TKTGLMLGLGETLDEVRDVIRELAGLGVDIVTLGQYLSPSKQHLAIERYV"
BASE COUNT         1820 a         3371 c         3261 g         1835 t
ORIGIN      
        1 gccacgcgct tctgaggtac gttcgcgcca gtgacggacg agaaacgcga gataaacgag
       61 cgagtactta ccgatgccag ccttcggtcg gagcgccgca agaccgacgc cgagctcgcc
      121 aagaagatgt ccatcgatga cgtcgccacg gacgttgtta tcgaggcgcg cgagaaagcg
      181 gacacgttga tgggcgaggc gcgcgcgctc gcagatctca aagccgcaga taacctgacg
      241 gctcgagacg cgcgagagat ccacgaagcg cgagcacgtg ctgatgccgc tgtagatgaa
      301 gcgagacgag gcgccgacac ggttgcgcgc gacgagcaca acgagcgcct ggtcgcgctc
      361 gccaggctgc tcgcaaccga acgccaagac accgatctca agctcgagct cgagcgcctc
      421 cacgcggacg cacaggtaac cacgcgcgag gacttcatgg cgatcgtgag ccatgacctc
      481 cgcagcctgc tcggtgggat cgcactcact gccgagctcc tgaagacggc cgaaagcggc
      541 ggcgacccgt tcgtgaaagt gaaggactac gccgggcgca tccagcgttt ctgcgcgcgg
      601 atgaatcgac tcgtcgccga tttgatggat gtggcgagca tcgaagccgg caagctgtcg
      661 ctcgttcgga agcgccgaga ggtcgcgctc ctattgcgcg acgccacgga ggccttcgaa
      721 cggaccgcgg cgtcgcaagg catcacgctc acgacgagct gcagcgcgcc cggaccgatc
      781 gacgtggatc acgaacggat ctttcaggtc ctgacaaatc tggtcggcaa cgccctcaag
      841 ttcacgccgg ccggcggccg catctcgatc gcaatcgacc gcagtgaaga cctcatccgg
      901 ttcaccgtca gcgacacggg cgagggaatt cccgcgggca tcctcggcaa gatcttcgac
      961 cgtttcgttc aggtcgtcga gaacgatcgc cgtggcctgg gactcggact gttcatcgcg
     1021 aagtcgatcg tcgaggcgca cggcgggaaa ctgtgggttg agagcacccc cggtaagggc
     1081 agtacgttct acttcaccgt ggcggcgacg cgcgaggcgc ttgcctcgtc cagcgagtga
     1141 gacgcacgca cgtttgccgc ggtctcggtg tgcgcagcaa gcttgacgta aaacttcttt
     1201 acgactgcta gctcctccgg actgagctct tcgatatcga cgagcttgtt gttcgctgac
     1261 gcgttactcg cgatgagctc gttgagcttg agctgaatcg caagggtgtc gcgattctga
     1321 gagtgctgga tcacgaacac catgaggaac gtgatgatcg tcgtcgccgt gttgatgatc
     1381 atctgccacg cgctcgagaa cccgaagagc ggccccgttc cggcccataa cgcgacgagc
     1441 gcgaatgcgg ccatgaagcc gtacgagctc cccgcgcctc gtgtcacgcc attcgcgaca
     1501 gagccaaata ggtgcttgcg agtgcgtggg gaatcggtcg ttggcggcat cgtctacggc
     1561 ttgacgatcg tgttgttcgc aagggtcacg cctgcgatgc gtgccaacgc tcggccgttg
     1621 agcgtcgcgc cgttctctag cgtgatcgct tggtcggcca tgatcgttcc acggaacgcc
     1681 gacgtggtgc cgagggttgc cgagctgccc acctgccaat acacgtttcg tgcgttcgct
     1741 cctccggcga gaatgacctg gcgcccggat gttgtcgtca acgttgacgc catctggaag
     1801 atgaacacgg cgtcgtcatc gccctgtgca tcgagcgtga gatcgcccga tgaaatctcg
     1861 agcgacgagg tcgacttgta gaggcctggc gcgagcgtca tgccgccgag gttgcctgcg
     1921 acggtgacag ggcaaagagt ccgtcccgca gcgtcgttgt atgcggtcgt cagatccgcg
     1981 tttgcggtcg ccgaggtcga gtccccggcg tgcatcgtgc ccacgagcgt gccgggtgga
     2041 aagcccgtca cagcagtgcc cgggctgaga ccgagatcgc cggtcaccga tgtcggcccg
     2101 gtgttcgtca ccgtcgagcc tgcgagcact ccgtaattac cggcaccacg catcgctacg
     2161 gctgcttggc tgcaggcgcc cgtcgtgaac gtccacacgt ggtcgctcgc gagcgtcgcg
     2221 ccttgcggat ctgcagcacc cgtcgtgatc cgagcggtgt aaacgagcga gacatcgagg
     2281 gggctcgacg ccacgaacgt cgcggtggtt cccgcgtagc tcacggtgcc tgcgatcggg
     2341 gtggtccctt gatgaaccgt gaatgtcgtg gacgtcagcg tggcgccgtt catcgcgcgg
     2401 ctgaacgtcg cggtgacgcg cttgttaacg gacacgttgt tggtggtgtc gaggggactc
     2461 gtcacggtca ccgtgggccg actaccgacg gcgtcaccgg aacctgaatt cgatcccgcg
     2521 tcgcgtccga actcgacgag ctgatctccg caagcggtga agctcagcgc aacgatcgcg
     2581 aacatggtgc aatggaggcg tttcatgatt acttcgcttt ctcgacgata atgaagctga
     2641 cgatgaactc gacgcggcgg ttggactcgc gtccagcaac ggtcgagttc gtaccggtcg
     2701 ggttatccga gccgaaccct ttggagatga ctcggtctgc cgcgacccca tgcgcgatga
     2761 ggtagtcgag caccgcttgg gcgcgtcgct ccgacagagt ttggttgtgg tcgtagttgc
     2821 cgtcggagga tgcgtggcct tcgacctgga ccttgaagcc ggggttgtcc ttcagtgcct
     2881 gcgcgacttc atcgagaagc gggaatgaca cgtcctcgag attcgcggag tcccacttga
     2941 aggcgatctt ctctttcagc tcgagcttgt cgcgcttcac gatcactttc gcgtacacgg
     3001 ggcaacccgc attgctgacg atgcccgcga cgtcggggca gttatcgtca tcgtcgacca
     3061 cggtgtcgcc gtcgcgatcc gatggtgacg gcggcggtgg tgtcacgtcg gccaccggag
     3121 tggtgacctc ttcctgcgca gtgaccggcg aacgacgccg atgcatgaga cccgggccga
     3181 cctcgaggct gagaccgatg ctcaggatct ttgcatcgcg gttgtcgaag ccggcgcggt
     3241 caccctgcac gatctgcgag taacgcgcga acggaccgaa ccagagcttg cggttctcgt
     3301 cgagcggcag cgcaactccg acggcggcgg cgaaccccgg gcggttcagc gggcccgtgc
     3361 ggacgtagtt gaggtcggcg tcaacccatg gcgagatggc gctaagacgt ccacccggag
     3421 catcgtgagg ccgctcgagg cgacccgtcg cgccgaacgc ccatgacgtg ccggcgtcgc
     3481 cggttccctc ggccggaagc gtcgtgaacg cgacgctcgg accgaaagcg aaatagcgag
     3541 acacgccgaa caagagcttc acggcctggc gaaagcctgc ctcggtcatc tcggactgcg
     3601 ggtgactcag tgcgccggca acgccaggct cgaccttcag cgaaagctgg agtcccgagc
     3661 caaatgttgg agcttcgttc tcgtcggcag gagcttcttg agctgctgcg accgagaccg
     3721 atccgaaata aaaagcgacg atagggatta gagcgcgtac gtacatggca cctcggcgcg
     3781 acccgatgtg cagcatccat acccgccgca acttgcgtgc gttgcgacgc tctcatcgag
     3841 ctcgccattg gagacgccca cgagcgccca agcgatgggc accgttcgcc cgcggcgacc
     3901 gagtattgcc gtcgagccag cagatacacg cggcacgtat cgtgcgtagg atcaccgaag
     3961 atgcacgact cacgcgcgat cggttcgaat ggctttcccg agctcgcgcg tgcgatcgtc
     4021 gctgccggtc acgacgtttc caagatcatg gatgcgcttg caacctcgct cacgaggacg
     4081 ctctgcgacc gctgctcgat cgatcggatc tcgagcggcg aagccgagcc tgccgcacca
     4141 cgcgcattga gcccgcgcca cgcgattctt tcactgcagg gcaccaagca tctaatcggt
     4201 agcgtcacgg tgatgcgcga cgacgcgtca tctccattcg acgggtgcga cttcgctgac
     4261 atcgagacgt gcatcacata tgcgagcctt gccgcggagg tcgcgcttca cctcgacgca
     4321 gaacgcaggc tgctacaggc cgagcacgaa cgcgccgcac tgttccatcg caacatgctc
     4381 ggcatcgtcg ggcatgattt gcgcgccccg gtctccgcga tcctcctcgg aacggagatg
     4441 atcgtcgccc agcaccaaga cgacccctcg attgccgtca tcgccacgcg gatcgtctcc
     4501 ttcgcgaacc gtattaccgg gatggtcgac caattgctcg acctcagtcg cgcgcagctc
     4561 ggtggtggca tcccactggc gcggtgcgcg acgcggctgg ggcccgtgct cgaatcggtc
     4621 gtcgctgagc ttgctgccag gtacccgcga aatcagtttc gtttgatcgg cgacgtagac
     4681 gtcaagggcg tctgggatcc ggatcgcctg cgccaggtcg ttgcgagcct gctcacgaac
     4741 gccgtccgcc acggtttaga ggacgggccc atcaacatca tcatctctcg cgtcggaaat
     4801 gaaacgtcga tcgcgattca caacgagcta ctgggcgagc cgattccgca ggaagagctc
     4861 cgcggcttgt tcgaggcgta ccggcaaggt gagggagagc atcacggtcc aggtctagga
     4921 cttggtctct acatcgtccg tgaaatcgtc gaagcacacc gcggcacgat cgcggtggag
     4981 tccacgacga gcggcacgac atttcggatc gtgctgcctg agtgatgcgc tagttgttct
     5041 gcgacggcgc gtcggtcgtc gtagccgtgc tcgcgtcggt cgtcgtagcc gggctcgcgt
     5101 catcatcgtg gagccgtcga tatagcgagc gacgatcgat accgaggatc ttcgcagctt
     5161 cgctcttgtt gttgcgtgtc acttcgagca cctgttgaat gtagcgacgt tcgagctctt
     5221 cgagcgtcac gagctcgtcg gacgcgccgg tcgcggtccg tgtccgggaa ggtacgtagt
     5281 ctcgaatcgc ctctggaagg tcgcgtgctg agatcgagct cgactcgagg agtgcgatca
     5341 cgcgctcgat acaattctgc agctcccgca cgttgcccgg ccagtcatag gcgatcagct
     5401 tgcgcactgc atcgtcctcg agcttgggag ccggcttgcg cgagcgcgcc gacagctgct
     5461 cgatgaagcg ttgggcgagg agccgcacgt cacccggccg gctgcgaagc ggagggacct
     5521 tgatctcgac gacgttgatg cggtgataca gatcctgtcg aaagcgctgc tcgtcgatcg
     5581 cggttcgcag gtcgcgattc gtcgcagcga tcagtcgcgc gccgaacgtc acttccgcgt
     5641 cgccaccgac ggggcgcagc tttcgctcct gcaggacgcg cagcagcttg acttgcatct
     5701 cgagcggcat ctcgccgatc tcgtcgagga acaacgtccc ctcgccggcc tgcacgaaca
     5761 acccatggcg gttctgtcgg gcatccgtaa acgcaccgcg tacgtgaccg aacagctcgc
     5821 tctcgagcaa cgcgggcggc agtgcgccgc agttgatcgc gacgaacggt tgatgtttcc
     5881 gatccgagag atcgtgaatc gcgcgagcga cgagctcttt gccggtgccg ctctcgccgg
     5941 tcacgagcac ggtcgacggg ctctccgcga cgcgccggat catcgaagtg acctctcgga
     6001 tcgcggcgct gtcgccgagg atcgcgtcga cgggctggga tgcggcgacg gtgaggcgca
     6061 agcgctgtac ttcacgacgg agcgcctgat aattgacagc gcgctcgatc gcgacctcga
     6121 ggaccgacag cgttacgggc ttgcagatga aatcgtaggc gcccgcgcgg atcgcctgca
     6181 ccgcagtctc gaggctgccc tgcgacgtga cgacgatccc gagcgtttcc agatgtcgat
     6241 cgcgaagccg cgatacgagc tctaggcccg acacgtcgcg caggtggaga tgcgcgacaa
     6301 cgatatcgac cggattgcgg tcgagaaacg cgatcgcttc cttcgccgta cgaaccgcct
     6361 ccgccgagta cttgcgatct cgcaagccat cccggacgag ctccgcgaat tcgcggtcgt
     6421 ggtctacgac cagaatgcac gtcacgcagc cactcttaca ccattagaca aaaccgcaac
     6481 gtcagaaagc aatccccggg ccaccagtta actactcgca cgtccacgat tcgtcgggcg
     6541 cgcgtaacaa gccgtgggca gcgtgtgccc acgttccgcc cagcaggtct tcgaggttgg
     6601 gcgttctggc gatgtgtcgc agcggtatgg aatgtgctcc tcggtgcgag cccgtgaccg
     6661 ctccacccga tatcgccctc gacccgttcg acgggcacta cgagctcggg gagctgctcg
     6721 gaagcggtgg gatggggaac gtgtacagcg cgatgcaggt ttctctgggt cgccgcgtcg
     6781 ccatcaagct gccgcaacgc cacctcgtct ccaatcccct cgtgctgcgc cggtttcgag
     6841 ccgaagccct cgccggcagc cggatcgatc accgcaacgt cgcacgcgtc atggattttg
     6901 gagagcgcaa cggcgtgttg ttcctcgtca tggagtactt gccgggagtc acgctcgaca
     6961 gcttgttgct cgagcatggc cccatgctgg gcagcgctgg cccggacctc tgcgggcaac
     7021 tgctcgatgg tctcgccgcg gctcacagtg ccggcgtcat tcacgcagac atcaagagcg
     7081 cgaacctcat cgtcgagatg cgagccgatg gtccgatcgc acacgtgatc gacttcggcc
     7141 tcgcgcggtt cgtgaacgcc cccgagctcg aagtcgaacg tcttctctcc gggaccccgg
     7201 agtacctcgc gcccgagctg atcaccggcg ggctgccatc ggtagcgtcg gatctctacg
     7261 ccgcaggtat cgttctctac gagctcctga ccggcgcaac tccgttcgcg gggggaacga
     7321 gcgacgagat cttgcgtcgt cacagcgagg acgccgtcgt cccgccgtca ctgcgctctc
     7381 cggatcaagg tatccatgct gcgctcgacg cagtcatcat tcgcgcgttg gcgaaggaac
     7441 cggctgcacg attcgaaacc gccgcaagct tcgcggccgc gcttcgggca tgtcctcgta
     7501 cactcgcggt cgcgccgcgc gcaaagcgtg aaaccgcgcc cccgttctct accgagtcac
     7561 ccacgcgcga tctccatccc gaaccgcccg aagatgtggc gagcgacacc gatcgaatcg
     7621 cgctgattcg ggccgcaatc acggaggcct tcgcgcgcaa tgatggagac ctgctcgtaa
     7681 cgtcgtatct cgagctggcg cgtgcgttga tcgacgatca cgacctgccc cgcgcaatcg
     7741 cggagctcga acgaggtctg cgaatgcttc gatccacggc gcgcgagacg gcctcgggcg
     7801 cctggcgcct tcaattgtgt ctcgcagcgc tgtactcggg aactgcccaa ccggaacgcg
     7861 cacgcaacgc agcgactctc ggtcgcgaag acgccgcccg cgctgcatcg gagctcggga
     7921 agactcgcgc tgaatcgttg ctcgctcgat tgaagcgcag cagcgcgcgt acatagcgcc
     7981 cacgagatgg gcagcatgcg cccgcgacct ccttcaccga cgacggcatg aatcgtgcgt
     8041 tcagctcgcg catggacgac gacaacgacg cacgccacga gaatggcgtc gacgccaccc
     8101 gcggacaacc gatcctcgac cgcgtgctcg ctcacaaggt cgtgctcgag acaaagcttc
     8161 agacgctctc ggatgacgat aggacgacgc gggcggacat cgagctcgcg ctcgcaacag
     8221 tccgcgagct actcacgggg cgtttgaccc acttgcctcg cgtcgtcgcg gtggacctga
     8281 accgttggct cgaacgcagc aagcacctcg gcggctagcg cacgccacta cctcgtgccg
     8341 tacgtctcgg cgagccagct ctcgaacgtc gtcttgccga tcgcgcgatc gggacagcac
     8401 agctttcctg cgcgcagaaa tgcgccggta gcgccgggca ccggcatccg cacgacgagc
     8461 cgcttcttgt gtgccgcgcg cttccaccgc ctcgcgagat cgccgaagtc gcgcacctct
     8521 ggcccgccca cgtcagggat gcgcttctgc ggtccagcag cgacagcgtc gacgagcacc
     8581 gctgccacct cgcgaacatc gatcggttga atcttgaagc cacgcggcgc gaagacgatg
     8641 ccgagctttg gcgcggcgag cttcggaatc agatcgtgga actgcgtcgc gcgcagcagc
     8701 gtccacggca cggcgctcgc ctcgatgacc ttctcctgcg cgactttcgt gcggtagtac
     8761 gcgagcggcg cgtcgtcgat accgacgatc gagataccga cgaagtgcct cacgcccgca
     8821 gcggctgcag cctcgagcac acgtttggtc ccgccgacga gcacgtcgtg cgcaccggcc
     8881 tgcgcgttcg tcgcatccat gatgacgtcg acgcccgcaa acgcctcggc gagtccctgc
     8941 cccgtcgtga cgtcgacctt gaccgcgccg ctgcgactcg cggcaacgac gtcgtgcccc
     9001 gcggcggtcg ccttctcgac gatggaacgg ccgaggctgc cagttccgcc gaggactacg
     9061 atcttcacgg gttgttgccg gtgggtggcg gagcactgcc ctgcatcgcc ttgaccatgc
     9121 agtcgcccat ctgcgtgccg atctgctgca tctctttggt ctgcgcgtcg gtcatcttcg
     9181 gcggctcgtc ggacttcttc gccatgtctt gcgcccacgc ggtcatgtcg tcggacacgc
     9241 gacgcgcgca gtcggagtcc ttgcacatgc agaactgatc gcgaaacccg cgcatcttgt
     9301 ccatcgcgag ctcggtctcg gtcttcggtg tgggcggcgt ggcttccgcc ggcgcgctgt
     9361 tcgcgagcgg cggtgtgctc gtcggtggcg aggccgtctg cttctgccca cctccgcagc
     9421 ccacgaccgc caacgcaccg aagaacagca tctttttcat gcgtctatgc tatccaacaa
     9481 ccgatgtccg agcgcgtgca cctgccagta gttcaggccg aggacggcgc agccgcgcct
     9541 gccgcgccga cgcaggtcgt tcgccgccgt catccagagt ggattcgcgc gcagctgccg
     9601 acgaagcccg cgtacttcga gctccgcgcg atggtcaaag agctgaagct ccacaccgtc
     9661 tgcgagagcg cgagctgtcc gaacatcggc gagtgctgga cgcgcaaggc actgacgatc
     9721 atgatcatgg gcgatatctg cacgcggtcg tgtcgcttct gcgacgtgac gacgggccgc
     9781 ccgctgccac tcgatcccga cgagccgcgc cgcgttgccg agatgctcgg ccgcctcgac
     9841 ctcgagcaca ccgtcatcac gtgcgtcgat cgcgatgacc tcgcggactt cggcgccgcg
     9901 cactgggccg agacgatccg ccgcgtgaaa gatgcgtgcc ccgagatgac gctcgagatt
     9961 ctcgccggcg acttcaaggg caaacccgag ctcgtcgaca tcgtgctcga cgccgatccg
    10021 gacgtcttcg cgcacaacct cgagacggtg ccgcgcctct cgcgtcaggt gcgcgttcaa
    10081 gcaagctacc ctcgcagcta caagatcctc gagcacgccc gcaagcgcgg cgccgtcacc
    10141 aagacgggct tgatgctcgg cctcggcgag acgctcgacg aagtgcgcga cgtgatccgc
    10201 gagctcgcgg gcctcggcgt cgatatcgtg acgctcggcc agtacctctc gccgagcaag
    10261 cagcacctcg cgatcgagcg ctacgtc
//
LOCUS       DATLBZ010000457        11361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_579690, whole genome shotgun sequence.
ACCESSION   DATLBZ010000457 DATLBZ010000000
VERSION     DATLBZ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11361
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579690"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VMZ53_23015"
     CDS             complement(<1..705)
                     /locus_tag="VMZ53_23015"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HUS31409.1"
                     /translation="MAKAEKVTLELGGIEVALSNPAKLYFPKAGITKQELVDYYLAVE
                     PGVLRALARRPMILKRYVNGAESEPFYQKRAPPKRPPWLDIATFRFPSGRSADEIVVN
                     HAASLVYVVNLGCIELHPHAVRSDDMDRPDELRIDLDPVPGVAWPQIIDVAKVAREVL
                     DELGLVGWAKTSGSRGAHLWVRIHPRWPFTEVRRAALAFAREVERRAPSIATAKWWKE
                     ERQGVFLDYNQNARDRT"
     gene            737..2107
                     /locus_tag="VMZ53_23020"
     CDS             737..2107
                     /locus_tag="VMZ53_23020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HUS31410.1"
                     /translation="MQSRWSTRLTTLLALAIPMVLARMTQSVITFADAIQVEHLGPQS
                     LAATATGGLNVMGLVILPQGLAFIIQSFVAQLAGRGDRAETPRFAIYGLGIALVAGII
                     GLAAIPAIDPLLSLTSYSPAVRQEMSDYMMIRFLAVAAMVGTEALGNWYGGLGNTWMQ
                     MVAGVITMVAAVFCNFLLIDGHLGAPALGVSGAAWAAVIGSWLGFAFLAIAFWRGWGN
                     APRTKGHLRHQLSKKEFVRVVRFGLPNGANWFLEFAAFQIFINIVMSSLGDETVAALN
                     AVLAVNTIGFMPAFGLASAGAILAAQSIGAGKRDEVWPQVKTTLLCTLTWMGVMGVVY
                     LVWPESILALFDSKHVSTNMVTIGTTMLLISAAWQLFDATSMTLSETLRAAGDTFWTA
                     AARLILAWVVFTPAAILVVMRWDGGPVGAMLCLVGYLAVLAALLGYRFRSGAWRKIEL
                     IEPKLV"
     gene            complement(2108..3382)
                     /locus_tag="VMZ53_23025"
     CDS             complement(2108..3382)
                     /locus_tag="VMZ53_23025"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HUS31411.1"
                     /translation="MKTKHISKILFAVTLGSSAVASANGFNVNEHDARVTGRGGATAA
                     SNDTPSSIVFNPGGIPIAEGTNFSVGLTSYIASGSYTPEGGGAKTTTDSQPAFAPSLY
                     VTSRVHDKVAVGIGLHFPFGLAVSWPTGHMQSDTIQDQSLRTYFITPAFGLNLNKEIP
                     GLTIGGGVDIVPATVQLERTITFGDAQGTAVLGGDALGIGGRFGVMYAPPKAKQIKLG
                     AMWRSDVNLDFSGNGDFDIDPQYRGQLPPDGKIATTIHLPQSIAGGIAFDPNPELEIE
                     MNAVWINWEKFNELRITLPDGSETNTVENYHNTVTFRIGADYKLAKQNAAIRAGFIYD
                     PTPIPDTTLSAQLPDVNRKNITIGGSKYFGAYAAHAAFEWVTPGERKTSSTMYTPEFK
                     GTYGVQAFVFSLNIAGHFGGGASQPAPAAVAE"
     gene            complement(3389..5791)
                     /locus_tag="VMZ53_23030"
     CDS             complement(3389..5791)
                     /locus_tag="VMZ53_23030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31412.1"
                     /translation="MKKLACLLVLAASGCPDVKIDPDETGGPVVEFDPARSQATGARF
                     LPFPNDLVRDPTTGKLNLSAQACESATSKATRENILNKLDGFGTYETAIAITFTAEVD
                     AATLDGNVVLYQMTNQGTALDAASAVSVPLLLRKTTSARLLPDACTSPELVNAVVAIP
                     MVPLKQKSTYVAAVLKGVKDTTGNEFSGSATWGLVNAAQAPVTLDDAGNVIGERTPLD
                     PSDPEQLAQLKSLDLLWKAHAKALTFLDAVPNARPRTDNLVAFQFTTQTVTDPLDPTV
                     MGSPAQKLSTASPLLDPATQTGKFGPFSAICSANAETNATQCFLKLALGGCDPLGTGC
                     SNANYAAGNAACTLFGCGFVGDVLGTGIGTINYQQQLPNAWANANKKPLQGPWSDPLK
                     PETQASLVLETLITIPTGTMPANGWPVLIYGHGLTLSKETALTFAGRAAQAGFATVAI
                     DFAGHGSRAIRISDDVALGCTGRCVSGTTGEFLDPVDTCESVADCPNPTTDHCGRMEI
                     KGTDPAVAVAVGAPPTPRNNPQCYDPFLSTDLAKTRDGLRQSVLDLERVVLALKSCSA
                     GCGALTVDPTKILYAGVSMGGILGSIATAESPDIKAAMLSVPGAGWIDLLENTDTVEF
                     RCPLVNGLIDAGVLQGIKWTGTNTDALCLAADKLAWQGQPGYGTFSATARWVLDPADP
                     ANFASKLATKRFMIQEVTGDTVVPNITTQRLAALTGMTSKLQNADTINLGAPAPSAAV
                     VSPTPTENKYIKYTNDSTAFYVHSSLIKPPDVPAAVGAPKAQAGTLRMQIDAATWLGA
                     NK"
     gene            complement(5969..6916)
                     /locus_tag="VMZ53_23035"
     CDS             complement(5969..6916)
                     /locus_tag="VMZ53_23035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HUS31413.1"
                     /translation="MQVKRFAKRLGKELIDDGITDIGAMMAYYAVLALFPMLVFVLSL
                     ALLVLDDSTVRDGVTFALSAAPQSVRPLVLDRVTQLVEASSAGFAFVGAALALWGASR
                     GAVGLMTALNTIFEKKETRSWLRRQLIAIAVTLGVAILIVAALGLLFLGPFVGHFLAD
                     RFGLGDAFDVVWGIGRWVGAGVLVMLVWAVLYKFLANTDAPFRIFTPGAIVGVIAWLG
                     ISALFGLYLGHFNSYEATYGTLGGAIIFLTWLWLSNMTLLLGAEINDVLADFKAHEDP
                     AAAALSDEGEHGGPPATPATVKQEPNWARAAKEGHAKPA"
     gene            complement(6959..7032)
                     /locus_tag="VMZ53_23040"
     tRNA            complement(6959..7032)
                     /locus_tag="VMZ53_23040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6996..6998),aa:Arg,seq:tct)
     gene            7092..7799
                     /locus_tag="VMZ53_23045"
     CDS             7092..7799
                     /locus_tag="VMZ53_23045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31414.1"
                     /translation="MRTTIAILLSIYLAACSKGANEGAEEAKKQAEREQAQKAKEGGV
                     AQKISPPVTGKQKLDCNKVIKADLFQQALGETETVTVRASDSEPDAAASCDLLRGGKK
                     MTVEEQKAKLKKEPRLGVLSGDPICNVSTFCWTIEDPERFRKRCAELKEKDDDSLGFY
                     SCVLVVAQGADDVKRFRFFDDDTKCIIQVRGGPSNVDNDLIGKCAKAAHDLIGPDEIK
                     PGAAPAPAAPEAAGSGS"
     gene            complement(7817..8989)
                     /locus_tag="VMZ53_23050"
     CDS             complement(7817..8989)
                     /locus_tag="VMZ53_23050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31415.1"
                     /translation="MRAVGLLLLLGGCDNLFALDHLRDPDPGSDVDAAVSKLIDASSD
                     CPGGAFYGSYGAGGKGLLKLCLPAGPVTTLAFAGTVDTDNSLSCTSVATIDDVGTQIC
                     IKYATTIALASVRATGSRALVFVAADNMTVSGEVDVSSRQGEDRGAGGNWSNCQSSNG
                     KSGMYGGSGGAGGSFATRGGDGGTNASGAFTGSRATALPDRVRGGCAGGNGGDGPTSV
                     IAGAMGGKSGGAVYFIAGQELSFSDAAIVNASGMGGRGAMATSVTASTDSGGGGGGGG
                     GSGGLIGLDAPLIRIAAGATFFANGGGGGAAASVRVPGGNGAEPVATAITTAAMGGTN
                     TTFYAGHGGDGAVKATTGVNGAGMKSGGGGGGGMGYIVVFSPSGVPTTGKFSPSPQ"
     gene            complement(9005..10090)
                     /locus_tag="VMZ53_23055"
     CDS             complement(9005..10090)
                     /locus_tag="VMZ53_23055"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HUS31416.1"
                     /translation="MKLALPFQPPVEPMLAKLANEIPEGDQWIYEPKWDGFRAMVFKS
                     GDELYIQSRDLKPLGRYFPELEPQLRASLPDKCVLDGEIVIATDGKLDFEALLLRIHP
                     AASRVKMLAEQKPSSYVAWDLLALGDEDLRGLSQQERRTRLEAALADVKPPVHVTPAT
                     RDRAVAADWFARFEGAGLDGVMAKPQDLPFTAGKRVMMKIKHQRTADCVVAGFRWYKN
                     GKGTLVGSLMLGLYDGDGTLHYVGIASSFKQTERANLVKLLEPVRENAKEQHPWKDWA
                     EWQDQETDRRLPGATSRWNRGKDLSWEPLRIELVAEVSFDHMQGTRFRHATHFKRWRP
                     DKPTSDCRYDQLEVSPPQEIKEIFGRT"
     gene            10117..10443
                     /locus_tag="VMZ53_23060"
     CDS             10117..10443
                     /locus_tag="VMZ53_23060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31417.1"
                     /translation="MRAALVLTLTLLLVVDATPAHADRDMCTPGTKYRGATIDLDVKD
                     ADIHDVMRLLADVGNVNIVVADTVTGKVTLKLKRVAWDLAACTIARVHKLEMRVENTV
                     VLVRKL"
     gene            10542..10799
                     /locus_tag="VMZ53_23065"
     CDS             10542..10799
                     /locus_tag="VMZ53_23065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31418.1"
                     /translation="MTTFETIDISALDLVQGGADEGQGAPNREQIRIGVQAQGDRTGG
                     RANIGVEGERSRTNYAVCVQETRQAGGTPRDIRETCGLPPS"
     gene            10778..11086
                     /locus_tag="VMZ53_23070"
     CDS             10778..11086
                     /locus_tag="VMZ53_23070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31419.1"
                     /translation="MWPSAFVIGKETIMIDIALDLLALVTGAGESTTEVNVGPIRYRS
                     STTDYRTCVDGMRGAVQQQYPDQRSFFGRLFGTTDPNAAARGRAEQEAIASCGPPPAN
                     "
BASE COUNT         1959 a         3685 c         3727 g         1990 t
ORIGIN      
        1 cgtgcgatcg cgagcgttct ggttgtagtc gaggaacacg ccctgacgtt cttccttcca
       61 ccacttcgcg gtcgcgatcg aaggtgcacg ccgctcgacc tctcgcgcga atgcgagcgc
      121 agcccgacgc acctcggtga atggccagcg cggatgaatg cgaacccaca gatgcgcacc
      181 gcgcgagccg ctcgtcttcg cccacccgac gaggccgagc tcgtcgagca cttcgcgcgc
      241 gaccttcgca acatcgatga tctgcggcca cgcgacgccc ggcaccggat cgagatcgat
      301 gcgcagctcg tcgggacgat ccatatcgtc ggagcgcacg gcatggggat ggagctcgat
      361 gcagccgagg ttgacgacgt acacgagcga cgctgcatga ttgacgacga tctcgtcggc
      421 ggagcggccc gacggaaacc gaaacgtcgc gatgtcgagc catggtgggc gcttcggtgg
      481 agcgcgcttt tgatagaacg gctctgactc ggcgccgttg acgtagcgct tgaggatcat
      541 cgggcgccgc gcgagcgcgc gcagcacgcc aggctcgacg gcgaggtagt agtcgacgag
      601 ctcctgcttc gtgatcccag ctttgggaaa atagagcttc gccgggttgg agagcgcgac
      661 ctcgataccg ccgagctcga gcgtcacctt ctcggctttc gccatgcgcg aatcctggca
      721 tagttcgcct cctgccatgc agtcgcggtg gtctacacgc ctcaccacct tattggcgct
      781 cgcgattccg atggtgctcg cacggatgac gcaatccgtg atcacgttcg ccgacgcgat
      841 ccaggtcgag cacctcggcc cgcagtcgct cgccgcgacc gcgaccggcg gcctcaacgt
      901 gatgggcctc gtgatccttc cgcagggcct cgcgttcatc atccagagct tcgtcgcgca
      961 gctcgcgggc cgcggcgatc gcgccgagac tccacgcttc gcgatctacg gcctcggcat
     1021 cgcgctggtc gcgggcatca ttggcctcgc cgcgattcca gcgatcgatc cgctgctctc
     1081 gctgacgagc tactcgccgg ccgtacgcca agagatgtcg gactacatga tgatccgctt
     1141 cctcgcagtt gccgcgatgg tcgggaccga ggcgctcggc aattggtacg gcggcctcgg
     1201 caacacgtgg atgcagatgg tcgccggcgt gatcacgatg gttgcggctg tcttctgcaa
     1261 cttcctgctc atcgacggcc acctcggcgc acccgctctc ggcgtgagcg gcgccgcgtg
     1321 ggcggcagtc atcggcagct ggctcgggtt cgccttcctc gcaatcgcat tctggcgagg
     1381 gtggggcaac gcgccgcgca cgaagggcca cctccgccac cagctctcga agaaagaatt
     1441 cgtgcgcgtc gtccgcttcg gcctgccgaa tggcgcgaat tggttcctgg agttcgccgc
     1501 gttccagatc ttcatcaaca tcgtgatgtc gagcctcggc gacgaaaccg tggccgcgct
     1561 caacgccgtc ctcgccgtga acacgatcgg tttcatgccc gcgttcggcc tggcgtccgc
     1621 aggtgcgatc ctcgcggcgc aatcgatcgg tgcaggtaag cgcgacgagg tctggcctca
     1681 ggtcaagacg acgctgctgt gcacgctcac gtggatgggc gtgatgggcg tcgtctatct
     1741 cgtgtggccc gagagcatcc tcgcgctgtt cgacagcaag cacgtctcga cgaacatggt
     1801 gacgatcggc accacgatgc tgctcatcag cgcggcgtgg cagctgttcg acgcgacgtc
     1861 gatgacgctg tccgaaacgc tacgcgccgc gggcgacacg ttctggacgg ccgccgcgcg
     1921 actcatcctc gcgtgggtgg tcttcacgcc cgccgcgatc ctcgtcgtga tgcggtggga
     1981 cggtggaccg gtcggcgcga tgctctgcct cgtgggctac ctcgcggtgc tcgccgcgct
     2041 gctcggttac cgcttccggt ccggcgcgtg gcgaaagatc gagctgatcg agccgaagct
     2101 cgtctagcta ttcagcgacg gccgcgggcg ccggctgcga agcaccaccg ccgaagtgac
     2161 cggcgatgtt gagcgagaac acgaacgcct gcacgccgta cgtgcccttg aactccggcg
     2221 tgtacatcgt cgacgacgtc ttgcgctcgc cgggcgtgac ccactcgaac gccgcgtggg
     2281 cagcgtacgc accgaagtac ttgctgccgc cgatggtgat gttcttgcga ttcacgtcgg
     2341 gcagctgcgc agagagcgtc gtgtccggga tcggcgtcgg gtcgtagata aagcctgcgc
     2401 gaatggcggc gttctgcttc gcgagcttgt aatcggcgcc gattcggaac gtgacggtgt
     2461 tgtgatagtt ctcgaccgtg ttcgtctcgc tgccatcggg gagcgtgatg cgcagctcgt
     2521 tgaacttctc ccagttgatc cagacggcgt tcatctcgat ctcgagctcg gggttcggat
     2581 cgaacgcgat gccaccggcg atcgactgcg gcagatggat cgtcgtcgcg atcttgccgt
     2641 caggcggcag ctgaccgcgg tactgcgggt cgatgtcgaa gtcgccgttg ccggagaagt
     2701 cgagattgac gtcgctgcgc cacatcgcgc cgagcttgat ctgctttgcc ttcggcggcg
     2761 cgtacatcac gccgaagcgg ccgccgatgc ccagggcgtc gccgccgagg accgcggtgc
     2821 cttgcgcatc accgaacgtg atcgtgcgct cgagctgcac cgttgccggg acgatgtcga
     2881 caccgccgcc gatcgtcagg cccggaatct ctttgttgag attgaggccg aacgccggcg
     2941 tgatgaagta cgtgcgcagg ctctggtctt ggatcgtgtc ggactgcatg tggcccgtcg
     3001 gccacgacac cgcgagaccg aacgggaagt gcaggccgat gccgaccgcg accttgtcgt
     3061 ggacgcgcga cgtcacgtag agcgacggtg cgaacgcggg ctgcgagtcc gtcgtcgtct
     3121 tggcgccgcc tccttcgggc gtgtacgagc cgctcgcgat gtacgacgtc agaccgaccg
     3181 agaagttcgt gccttctgcg atcgggatgc cgcccgggtt gaacacgatt gacgacggcg
     3241 tgtcgttgct cgctgcggtt gcgccgccgc ggccagtcac gcgtgcatcg tgctcgttga
     3301 cgttgaagcc gttcgccgat gcgaccgccg aggagccgag cgtcaccgcg aagagaattt
     3361 tagagatgtg tttcgtcttc atggtgacct acttgttcgc gccgagccat gtggccgcgt
     3421 cgatctgcat gcggagagtg cctgcttgtg ccttcggggc gccgaccgca gccggcacat
     3481 caggcggctt gatgagcgac gagtgcacgt agaacgccgt cgagtcgttc gtgtacttga
     3541 tgtacttgtt ctccgtcggc gtgggcgaga ccaccgcagc ggagggtgcc ggcgcgccga
     3601 ggttgatcgt gtccgcgttc tggagcttcg acgtcatgcc ggtcagcgca gcgagccgct
     3661 gggtcgtgat gttcggaacg acggtatcgc ccgtgacctc ttggatcatg aagcgcttcg
     3721 tcgcgagctt gctcgcgaag ttcgccgggt cggccgggtc gagcacccag cgcgccgtgg
     3781 ccgagaacgt gccgtagccc ggctggcctt gccacgcgag cttgtcagct gcgaggcaga
     3841 gcgcgtcggt gttcgtaccg gtccacttga ttccctgcaa cacgccagcg tcgatgagac
     3901 cgttgacgag cgggcagcgg aactcgacgg tgtcggtgtt ctcgagcaga tcgatccagc
     3961 cagcgccggg gacgctgagc atcgcggcct tgatgtcggg gctctcggcc gtcgcgatcg
     4021 agccgaggat gccgcccatc gagacgcccg cgtagaggat cttcgtcggg tcgaccgtca
     4081 acgcgccgca gccagcggag cacgacttga gcgcgaggac gacgcgctcg agatcgagca
     4141 cgctctggcg caggccgtca cgcgtcttcg cgagatccgt cgacaggaac gggtcgtagc
     4201 actgtggatt gttgcgcggc gtcggaggag cgccgacggc gactgcgacc gcaggatcgg
     4261 ttcccttgat ctccatgcgt ccacagtggt cggtggtcgg gttcgggcag tcggcgaccg
     4321 attcgcacgt atcgacgggg tcgaggaact cgcccgtcgt gccgctcacg cagcgacccg
     4381 tgcatccgag tgcgacgtcg tcagagatgc gaatcgcgcg gctgccgtgg cctgcaaagt
     4441 cgatcgcgac cgtcgcgaaa cccgcctgtg cagcgcgtcc cgcaaacgtc aacgccgtct
     4501 ccttggagag cgtgaggccg tgaccgtaga tgaggacggg ccagccgttc gccggcatcg
     4561 tgccggtggg aatggtgatg agggtctcga gcacgagcga cgcctgcgtc tcgggcttca
     4621 gcggatccga ccaaggacct tggagcggtt tcttgttcgc gttcgcccac gcgttcggca
     4681 gctgctgctg gtagttgatc gtgccgatgc cggtgccgag gacgtcgcca acgaacccgc
     4741 aaccaaagag cgtgcacgca gcgttaccgg ccgcgtagtt cgcattgctg catccggtgc
     4801 cgagtggatc gcagccaccg agcgcgagct tgaggaaaca ttgcgtcgcg ttcgtctcgg
     4861 cgtttgcact gcagatcgcc gagaacggac cgaacttgcc cgtctgcgtc gccgggtcga
     4921 gaagcgggct cgcggtcgac agtttctgcg ccgggcttcc catcaccgtc ggatcgagtg
     4981 gatccgtcac cgtctgcgtc gtgaactgga acgcgacgag gttatcggta cgcggccgcg
     5041 cgttcggcac ggcgtcgagg aacgtgagcg ccttcgcgtg cgccttccac agcaggtcga
     5101 gcgacttcag ctgcgcgagc tgctctggat ccgagggatc gagcggcgtg cgctcgccga
     5161 tgacgttgcc ggcatcgtcg agagtgaccg gcgcttgcgc cgcgttcacg aggccccacg
     5221 tcgcggagcc ggaaaactcg ttgcccgtcg tgtccttcac gcccttgagc accgcagcga
     5281 cgtacgtgct cttctgcttg agcggcacca tcgggatcgc gacgaccgcg ttcacgagct
     5341 cgggcgacgt gcacgcatcc gggaggaggc gcgcgctcgt cgtcttgcga agcaagagcg
     5401 gcaccgaaac cgcgctggcg gcatcgaggg ccgtgccctg gttcgtcatc tgatagagga
     5461 cgacgttgcc gtcgagcgtc gccgcatcga cctcggccgt gaacgtgatc gcgattgcgg
     5521 tctcgtacgt gccgaagccg tccagcttgt tgaggatgtt ctcgcgcgtc gccttggacg
     5581 tggccgattc gcaggcctgc gcgctgaggt tcaacttccc ggtagtcggg tcgcgaacga
     5641 ggtcattagg gaacggcaag aatcgcgcgc ccgtcgcttg cgaacgagcc gggtcgaact
     5701 cgaccacggg cccgccagtc tcgtcgggat cgatcttgac gtcaggacag ccgctcgccg
     5761 caagcacgag caaacaggct agctttttca tccagtgctc catcgaagga tgccgagtat
     5821 aggcacctct cacgaagcac gcgagcgatc ccaaccgcaa cgatctgttg tcgatctgcc
     5881 ctcaggaaga gaccgtgagc atgcccacgt cgcttacgac gccgcttcgc ggggcgtgta
     5941 gtgcatcacg cgagctttgc ggaccttgtc acgcaggctt tgcgtggcct tctttggccg
     6001 cgcgcgccca attgggctcc tgcttgaccg tcgcaggcgt cgcagggggg ccaccgtgct
     6061 cgccttcatc cgagagcgca gcggcagccg gatcctcgtg cgccttgaaa tcggcgagca
     6121 cgtcgttgat ctccgcgccg agcagcagcg tcatgttcga cagccacagc cacgtcagga
     6181 agatgatcgc gccgccgagc gtgccatacg tcgcctcgta gctattgaag tggccgaggt
     6241 agagaccgaa cagcgcgctg atgccgagcc aggcgatcac accgacgatc gcgccgggcg
     6301 tgaagatgcg gaatggcgcg tcggtgttcg cgaggaactt atacagcacc gcccacacga
     6361 gcatcacgag gacgccggcg ccgacccagc ggccgatgcc ccatacgacg tcgaacgcat
     6421 cgccgaggcc gaagcggtcc gcgaggaagt gaccgacgaa cgggccaagg aacaggagtc
     6481 cgagcgccgc gacgatcaga atcgcgacgc cgagcgtgac cgcgatcgcg atgagctggc
     6541 ggcgaagcca cgagcgcgtt tccttcttct cgaagatcgt gttgagcgcc gtcatgagac
     6601 cgaccgcgcc gcgcgatgca ccccacagcg cgagtgcagc accgacgaac gcgaatcctg
     6661 cactcgacgc ctcgacgagc tgggttacgc gatcgaggac cagtggccga acgctctgcg
     6721 gcgccgcgct caacgcgaac gtgacgccgt cgcgcacggt gctgtcatcg agcactagca
     6781 gggcgagcga caacacgaac acgagcatgg gaaagagcgc gaggaccgcg tagtacgcca
     6841 tcatcgcgcc gatgtcggtg atgccgtcgt cgatcagctc ttttccgagt cgctttgcaa
     6901 accgcttgac ctgcatgcac aaagaaagtg cagcgcatat gccagcgagc agcgcgagcg
     6961 cgcccggcag gagtcgaacc tgcaacctac cgggtagaag ccggttgctc tatccaattg
     7021 agctacgggc gcgtgcggga aactctgcac ccacaggagc ttcccggcaa gtcgtgttag
     7081 gtttccgtgc gatgcggacg accatcgcaa ttcttctgag catctacctt gccgcgtgta
     7141 gcaagggcgc caacgagggc gccgaggagg caaagaagca ggccgagcgc gagcaagcgc
     7201 agaaggccaa ggagggcggg gtcgcgcaga agatcagccc accggtgacg ggcaagcaga
     7261 agctcgattg caacaaggtc atcaaagcgg acttgttcca gcaagcgctc ggcgagaccg
     7321 aaacggtgac ggtgcgggcg tcggattcgg agcccgatgc agcggcttcg tgcgacctgc
     7381 ttcgcggtgg caagaagatg acggtcgaag agcagaaggc caagctcaag aaggagccgc
     7441 gcctcggcgt gctctccggc gatccgatct gcaacgtgtc gacgttctgc tggacgatcg
     7501 aagatccgga acggttccgg aagcgctgcg ccgagctcaa agagaaggac gacgactcgc
     7561 tcggcttcta ctcgtgcgtc ctcgtggtcg cgcagggtgc ggatgacgtg aagcgcttcc
     7621 ggttcttcga tgacgacacg aagtgcatca ttcaggtgcg cggtggtccg agcaacgtcg
     7681 acaacgatct gatcgggaag tgcgcgaagg ccgcgcatga tctgatcggt cccgacgaga
     7741 tcaagccggg cgcagcaccg gcaccggcag cgcctgaagc tgctggctcc ggatcgtgat
     7801 cgcgcgagcc gactagttac tgcggcgacg gcgagaactt gccggttgtc ggcacgccgc
     7861 tcggcgagaa cacgacgatg tagcccatgc cgccgcctcc gccgccgccc gatttcattc
     7921 cagcgccgtt caccccggtc gtggctttga ctgcgccatc cccgccgtgc cctgcgtaga
     7981 atgtcgtgtt cgttccgccc atcgccgccg tcgtgatcgc ggttgcgacg ggctccgcgc
     8041 cgttgccgcc aggcacgcga acgctcgcag ccgcaccgcc tccaccgccg ttcgcgaaga
     8101 atgtcgcgcc ggctgcgatc ctgatgagcg gggcgtcgag accgatgagg ccgccgcttc
     8161 ctcctcctcc tccgccgcct ccgccggagt ccgtcgacgc ggtgaccgac gtcgccatcg
     8221 caccacgacc gcccatcccc gatgcattga cgatggcggc gtccgagaac gagagctctt
     8281 ggcccgcgat gaaatagacg gcgccgccgg atttgccgcc catcgccccg gcgatgaccg
     8341 acgtgggacc gtctccgcca ttcccgcctg cgcagccgcc gcgcacacga tcgggcagcg
     8401 ctgtcgctcg cgagccagtg aacgcaccac tcgcgttggt tccaccatcg ccgccacgcg
     8461 ttgcaaagct tccgccggcg ccacccgagc cgccgtacat gccgctcttg ccgttactcg
     8521 attggcagtt gctccaattg ccacccgcgc cgcggtcttc gccctgtcgc gacgacacgt
     8581 cgacctcacc gctaaccgtc atgttgtcgg ccgcgacgaa caccaacgca cgacttcctg
     8641 ttgcgcgaac tgaagcgagg gcaatcgtcg tcgcgtactt gatacagatc tgcgtaccga
     8701 cgtcgtcgat cgtggcgacg ctcgtgcagc tgagcgagtt atcggtatcg acggtgcccg
     8761 cgaacgcgag cgtcgtgaca gggcctgcgg ggagacagag ctttagcagg cccttaccgc
     8821 ctgctccgta cgaaccgtag aacgcgccac cggggcaatc actcgacgcg tcgatgagct
     8881 tgctgacggc cgcatcgaca tcggagcccg gatccggatc gcgcaagtga tcgagcgcga
     8941 acaggttgtc gcagccgccg agcagcagca ggaggccaac cgcgcgcatt cctcattcta
     9001 accgttacgt gcggccgaag atctctttga tttcctgcgg cggcgaaact tcgagctggt
     9061 cgtaccggca gtcactcgtc ggcttgtcgg gacgccagcg cttgaagtgt gtcgcatgac
     9121 gaaaccgcgt gccctgcatg tgatcgaacg agacctctgc aacgagctcg atccgcagcg
     9181 gctcccaaga gagatccttg ccgcgattcc accggctcgt cgcgccgggc aagcggcgat
     9241 cggtttcttg atcctgccac tccgcccagt ccttccacgg atgctgctct ttcgcgttct
     9301 cacgaacagg ctcgagcagc ttcacgagat tcgctcgctc ggtttgcttg aacgacgacg
     9361 cgatgccgac gtagtgcaac gtgccgtcgc cgtcgtacag gccgagcatg agcgaaccga
     9421 cgagcgtgcc cttaccgttc ttgtaccaac ggaagccagc gaccacacaa tcggcggtgc
     9481 gctggtgctt gatcttcatc atcacgcgct tgcccgcagt gaacggcagg tcctgcggct
     9541 tggccatgac gccatcgagg ccggcgcctt cgaagcgcgc gaaccaatcg gcggcgactg
     9601 cgcgatcgcg cgttgcgggc gtcacgtgta ccggaggctt cacatcggcg agcgcagctt
     9661 cgagccgcgt gcggcgctct tgctgtgaca ggccgcgtag gtcctcgtcg ccgagcgcga
     9721 gcaagtccca cgcgacatac gacgacggct tctgctcggc gagcatcttg acgcgagacg
     9781 cagccggatg gatgcgcagc agcagcgcct cgaagtcgag cttgccgtcg gtcgcaatga
     9841 cgatctcacc gtcgaggacg cacttgtcag gcaggctcgc gcggagctgg ggctcgagct
     9901 ccgggaagta ccggccgagc ggcttcaggt cgcggctctg gatatacagc tcgtcaccag
     9961 acttgaagac catcgctcga aagccgtccc acttcggctc gtagatccat tgatccccct
    10021 cgggaatttc gttcgccagc ttcgcgagca tcggctcgac gggtggttga aaggggaggg
    10081 ccagcttcac gcgcgacacg ctacactgat ctggtcgtgc gcgccgcgct agtgctcacc
    10141 ctcaccctgc tgctcgtcgt agacgcgacg cccgcgcacg ccgatcgcga tatgtgcacg
    10201 cccggcacaa aatatcgcgg cgcgacgatc gatctcgatg tgaaggacgc cgacatccac
    10261 gatgtgatgc ggctgctagc cgacgtcggc aacgtgaata tcgtcgttgc cgacactgtc
    10321 accggcaagg tgacgctgaa gctcaagcgc gttgcgtggg atctcgcggc gtgcacgatc
    10381 gctcgcgtgc acaagttaga gatgcgcgtg gaaaatacgg tcgtgctcgt tcgcaagctc
    10441 tgatcgagag cgttgtcgcg ctgtcgtttg gcgagccgtg gcatggccac tgcactagcc
    10501 acttgacgag aacgcaaaca actctcgagg agaaaacgac aatgacgacc ttcgaaacga
    10561 tcgacatctc tgctctcgat ctcgtgcaag gcggcgctga cgaaggccaa ggcgcaccga
    10621 accgcgagca gattcgcatc ggcgtccagg cccaaggcga tcgcaccggc ggtcgcgcca
    10681 acatcggcgt ggaaggcgag cgctcgcgca ccaactacgc cgtctgcgtg caagagactc
    10741 gtcaagccgg tggaacgccg cgcgacattc gcgagacgtg tggccttccg ccttcgtgat
    10801 cggcaaggag acgatcatga tcgacatcgc ccttgatcta ctcgcgctcg tcacgggtgc
    10861 gggcgagagc acgacggaag tgaacgtcgg cccgattcgc tatcgcagca gcacgacgga
    10921 ctaccggacg tgcgtcgatg gaatgcgagg cgccgtgcag cagcagtatc cggatcagcg
    10981 gtcgttcttc ggccggctgt tcggaacgac cgatccgaat gcggctgcgc gcggccgagc
    11041 cgagcaagag gcgatcgcga gctgcggtcc tccgcccgcg aactaggcga ccttgccgcg
    11101 tccgcgtcgt cgtcgcccgg cctggcctcg tcgccggtct cgtcgtcgtg cacgtcgtcg
    11161 tacacgtaca cgtacacgtc tcacgtgcac gtgcacgtgc acgtgacacg aatgccgctt
    11221 tcctcgccga gcgggagctc gtcgtcgggc gaagagcacg gtgcaggctc gccagcgcgt
    11281 ctgacgacgc atcgacgagc acgagcacga gcacgagcac gagcacgagc acgagcacgt
    11341 gcacgtgaca cgtggacgtg g
//
LOCUS       DATLBZ010000458        14304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_581373, whole genome shotgun sequence.
ACCESSION   DATLBZ010000458 DATLBZ010000000
VERSION     DATLBZ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14304
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581373"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VMZ53_23075"
     CDS             <1..667
                     /locus_tag="VMZ53_23075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31420.1"
                     /translation="LFDVLFIVIFAALIRAAAVQQAAAQVPAPAKPQPPSPPVKLEVA
                     QLRAQAVSSVSSDIAGRPTVIVRISAAGSVTAIETATATAATSPTSPTSPTSPTSPTS
                     PTSATSPTSPTSLTAGKSETLDSPLLEHSPDPDVALAYLGDRAAELRICRIVAVHLAL
                     PDLAHHLVILAPEKPLADLPHALVEGLRRDVERCLHDQQGLAVIVDPSAVPAAAGSAA
                     HEQ"
     gene            657..1139
                     /locus_tag="VMZ53_23080"
     CDS             657..1139
                     /locus_tag="VMZ53_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31421.1"
                     /translation="MSSERKALAKDVAKELNAQRRKRKLATYSVFGALLAAAIFFARC
                     GMGWGVGGGKGDGIGDGNGTSKAKGSGSAAAQAQRCTVRVSAEGIFVDGVKKTRDEAV
                     AACKQTDGAMVTVTGDARQGDWDELRAALQAVRVKIYIRGELWDGSNTPAGSDAPNQP
                     "
     gene            1172..1426
                     /locus_tag="VMZ53_23085"
     CDS             1172..1426
                     /locus_tag="VMZ53_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31422.1"
                     /translation="MADLDPANLLEIRIETNDSGPWAEDAEWVFVMRDGTETRFGNGQ
                     ITLSAIQWLPGFDNDAVIRAMTCTDNETFIVWRRPEQPSN"
     gene            complement(1467..2744)
                     /locus_tag="VMZ53_23090"
     CDS             complement(1467..2744)
                     /locus_tag="VMZ53_23090"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HUS31423.1"
                     /translation="MRASWLVLLAGCYSAQAGSGAGSGAGSGVGSGAGSATSVVAQCP
                     CDESHTDPSVQAPPHDELTLTKTKFTDLPGWTDDKQSEALPSFLNSCAKLAKLADDAP
                     IGVDGHGGLAKQWRHACAAAAKVKAGDDKAARAFFEAEFVVWQAAGKNGVDGKLTGYC
                     VQQLHASRKKAGKFQYPILARPKDLAEIKLDDFVNDGHGRRIWGRFVDGGKVVPYYTR
                     VEIRKGALASQKLELMYADDPVDVLFAHIEGSAKAIMDDGTTVWLNFDGKNGRAYKGV
                     GKALKDLNLLVAPYNGTMPGIRKWFVDHPKRFDEIVDLNASYVFFEESKQPGATGSQD
                     VVLTPRRSAAIDRAFIAHSTPIWVDGRQPEVGKTGTVPYRSLLIAQDTGGGIMGAVRA
                     DIYWGDDKTAEELAGRMGGPGKYWLLLPKGVTK"
     gene            2926..2996
                     /locus_tag="VMZ53_23095"
     tRNA            2926..2996
                     /locus_tag="VMZ53_23095"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2958..2960,aa:Gln,seq:ttg)
     gene            3000..3071
                     /locus_tag="VMZ53_23100"
     tRNA            3000..3071
                     /locus_tag="VMZ53_23100"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3033..3035,aa:Gln,seq:ctg)
     gene            complement(3149..7111)
                     /locus_tag="VMZ53_23105"
     CDS             complement(3149..7111)
                     /locus_tag="VMZ53_23105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS31424.1"
                     /translation="MNHFASLLFIITMGACGNLGSCGACGSTGPLPNGKLPTDQTVEG
                     GAQVRVTPQGFSKLSSILPGLLNQQLSSGFCLPKGSAGVSLFNFDIATAKYCQSGGGC
                     SGGCKITAQVNTPNGLTTQVVGNNLRVNLSTKIDTTIPIDFVVFGVTVDSCNMRLQSS
                     NLNGSFDVGFGIKPADGELDIKLANINSFTTNLQWSGCGIISDIGQLLTTLIDAIPNT
                     IKGFLTPLLNPLIQSFIPNPLGVKGMVNIGSLLEGISPGTDGFMEARIVPGGYVGMTA
                     NGGMNMGVITGMNADEDPATRTVALDSEPALCVPPMPAPVFSLPTVARSGLGGNTFQL
                     APAGQFSGNPDPAADIAMGLSKTTLNLTGHHLVTSGGMCLGVGTSYIKQLNVGTIGIL
                     VPSLGELASDEGNDPLLLVTRPQKAVDFSIGTGTMTDPNLTIHLRNIEVDFYAFLYER
                     YVRAFTLELTMNVGINMTFEQPASGPAVIKPELVGISSSEVQVKVLNSQFVRETPQHL
                     EGVLPSVFDLVTPLLGQLPPIQVPSFAGFSLNNLSIQKVTTSQDEFLALYASLGSSPM
                     MRVAADNDPFMRHAVDLIDEDAPSLKVQSTGKARLLAVDTPPAEIIRGALVPGQDGQL
                     PSVTFDVDRYDTMGRELEWTWNINGGLYRPYQPGGQLIISDRAFAWQGKYEIGLKSRV
                     KGDYHTVSDEIRTAVVIDSVGPRVFEDKTEWDGDQMKIHVWDVVDGKDIEIAFGAVGD
                     DAPQTKWVPAREATLALITAKHLADLGNGDIAVFARDQQGNQTIAYVAPFHGQAGASG
                     CSCDSGKAPSAGSLALFGLVGLGLFGRRRRRSLRISPAAKRMMKKAAKQLGFVACIAL
                     LSAFIPACSCGKGAGQSCETAEDCGPDFCEEGQLPFCVDNECVCSDDIPPGRIGPYSD
                     VGVGGGAIWVSAYAESHGDLVVAQTTGGRIPNEAWEWVDGVPDGPVVIPGSKIRGGIE
                     EKGADVGMYTSIAVASDGTPMVTYFDVDTASLKFATKSGGVWTTHIVEQGTSKIDPGS
                     GGSLVGMYTSLTLRSDDGRPGVAYLAHVKDGAGERAEVRYAAAQTANPTSSADWQFWT
                     VDKANIPANPDEVYPLPGGLGLFIDSARNPTNQAPVVTYYDRTNGDLKVSKFNPTSGQ
                     FATAIVLDGTNGIDAGWSPSVGIDANGVVNVAYVGATSDDLKFTTDATNAPKETIDDG
                     YRIDGITVDGLPKPVYHFVGDDAGLVLAGANGPRVVYQDATTQEMLLAEKGQDGKWTH
                     TSIAGGPQVSPWPGAYGFFASAALSPTDIVMSTWVVNQPEDDNWVEVFLRPITIARQQ
                     "
     gene            7397..7470
                     /locus_tag="VMZ53_23110"
     tRNA            7397..7470
                     /locus_tag="VMZ53_23110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7431..7433,aa:Arg,seq:tcg)
     gene            7520..7591
                     /locus_tag="VMZ53_23115"
     tRNA            7520..7591
                     /locus_tag="VMZ53_23115"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7552..7554,aa:Lys,seq:ctt)
     gene            7625..9331
                     /locus_tag="VMZ53_23120"
     CDS             7625..9331
                     /locus_tag="VMZ53_23120"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HUS31425.1"
                     /translation="MAVFSFRSSGLLFLSQASRSGRFAQGGSRHPDRDGKLQPPWYLP
                     DREGNDGYEAQDNADAGQERDGPRICDSRCAKAERARSRDDGRLDRRRPALSQRSRAR
                     KAERTPRARSQGARTAWPRGLGFAARLEAVSAPLEVRYEDRRVGILRADASNRFCFRY
                     VEEWLGARDAFPVSIALPLGPHEESGGSAHSFFANLLPEGLVRQAVCRRLGISESNDV
                     ELLRAIGGECAGALSIVEAGAKLQAPDNEYEELDAQKLQSLADDDDRVVPLLVGGAQT
                     RLSLAGAQEKLPVALLDGKIHLPLKRSASTHILKLPNPRYAHLPQNEAFVLGLASHAG
                     LECASSELTRATKPQSLLVERYDRRRTDDPWPVRRLHQEDFCQALGLLPGQKYEQEGG
                     PSLAQAVAVVRTSVANPLHDVRRLIEWQAFNAAVGNSDGHGKNLSILYDDEGPRLAPF
                     YDLVSTREYANLDRLLAMSVGGRRNADELGYEQWVSLASELDMRDRVVLDIVGRTLET
                     VADILPRWTDTFRERYGRLPVLQTLPGRIDNQLRRLRRGIEKPAKTANARKVKGGKTK
                     KR"
     gene            9401..10966
                     /locus_tag="VMZ53_23125"
     CDS             9401..10966
                     /locus_tag="VMZ53_23125"
                     /inference="COORDINATES: protein motif:HMM:NF006029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HUS31426.1"
                     /translation="MGSAEFLSLVPLFLAALVPACLLVSSFTISRQRARSLPVASFVT
                     TMTAVITYLAVFLRVLLWAIGFDAREVIPTSVMHDVVQIDIVSGAMFVLVSTLAMIVV
                     RYSRTYLAGQAELDRYARYLLLTVASVTVLVTSNHLAVLMVAWFATDIGLHQLLTFYR
                     RRRQAIIVAHKKFLLSRVADACFVGSLVMIGGAVGTLRIDVVNTFARSQGELSPALHL
                     ATVLLVIGVILKSAQLPFHGWMLQVMEAPTPVSALLHAGVVNMGGFVMIRLAPLMAHA
                     SLAQNILIGVGLLSTIVASLVMVTRAAIKSSLAWSTIGQMGFMLVQCGLGAWHLALMH
                     VLAHSLYKAHTFLSSGSIVRQWRGAHLVHSPHALLWHLIIGTTVVCAAVAPFYYATAL
                     PTGHLASTTGSLAFVLALSFVPMIGRAIAAGKRAFVLAALLTVGASAAYFAFHVVFDL
                     VAPSIMETPPSELKWQIVVGGLIFLFVAQSIMQTSPNGRFANFIQPHLDSGLYIDDWF
                     TRVTFRLWPPALQ"
     gene            10963..13392
                     /locus_tag="VMZ53_23130"
     CDS             10963..13392
                     /locus_tag="VMZ53_23130"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HUS31427.1"
                     /translation="MSDLVGLREDAERACARIAPTWPLDRFIAVNPFWPLTNKPVPAV
                     AGDLAALSGARLLMPREWYAQEWRAGRFRSEHLKEAIAESGSNATEDDLIASFWIREQ
                     EPLRRPLVVDVMDSLLRREHELTWRDFVMERISRFCASYFDDGQAQINGVREGGLYAS
                     WLPQAKMDQLPNLFMKLNGYRSTVAKLPNTADEMIALGCADLGVPVAQRERYLAALLL
                     DVNGWASWCAYLRFTARQGSGDDVHIRELLAIRLAWEWILFLASDDSLRAEWRFAMTS
                     WPAIDRAARASRTGWTLQRAVEIAWISQVRSKLLQGFDAKPIAQPKLQAAFCLDVRSE
                     VFRRGLEALGQDIQTLGVAGFFGVPIEYVPLAADHGSPHLPGVLAPKYRVTDTGVESG
                     LETTRRARLDAAYAWKAFKSSSLSSFAYVDAIGLFFARKLFRDAWRPERYRDDEKERP
                     GLTEDEDAKRVPRLTSRVDGTALSADERCDIAAGLLKAMGLTRNFARLVLLVGHGSET
                     RNNAYAAGIDCGACGGHPGDINARAAAALLNDGPIRSGLAARGIEIPAWTFFVAALHN
                     TTTDEVTLFDVSAAAQTHQTDLDNVRAALERAGFAARRERAPRLGLAELTDAKLYAAV
                     VKRATNWAEVRPEWGLAGNAGFIIAPRERTRHMNLEGRVFLHDYRVEDDRDYSILEAI
                     MTGPMVVAHWINFQYYASTVDNARYGSGNKVLHNIVGGHLGVFEGNGGDLRIGLSLQS
                     LNDGEHWVHTPLRLAVFIEAPREAIDSVLEKHAKVKELVDNEWLHLYQLDVTERALYA
                     RRGGTWKPAPG"
     gene            13527..>14304
                     /locus_tag="VMZ53_23135"
     CDS             13527..>14304
                     /locus_tag="VMZ53_23135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31428.1"
                     /translation="MRRLLLLVVIAACGTTEDEVPTYGEGLGTPDSPIPQDGAPYTVH
                     TQTAAPSTTTQAEVTRIAGALRQFSQTPARSMLTLAKQASPEELATLDAMSSTLRSRL
                     EGWIDTEIDKVVVDGVRGRAVASEMADFVDSTLAHFSFESTLSFTPTKTTHTLGAVSF
                     RLSGLDVVVPVGGLAGDDAQQRVSATVGEGGSLAFGDHTFGIDFGAHAWHGINLASST
                     LHGVGMAPALSNAVNCKALAQAVSAKCYNGACVGHSAEITS"
BASE COUNT         2395 a         4541 c         4829 g         2539 t
ORIGIN      
        1 actgttcgac gtgctgttca tcgtgatctt cgcggcgctc attcgcgcag ccgcggtgca
       61 gcaagccgct gcgcaggtgc ccgcaccggc gaagccacag ccgccgtcac cgcccgtgaa
      121 gctcgaggtc gcgcagctcc gcgcgcaggc cgtgagctcc gtgagcagcg acatcgcggg
      181 acgcccgacg gtcatcgtgc gcatcagcgc ggcggggtcg gtgacggcga tcgagactgc
      241 gaccgcaacg gccgcgacga gcccgacgag cccgacgagc ccgacgagcc cgacgagccc
      301 gacgagcccg acgagcgcga cgagcccgac gagcccgacg agcctgaccg cgggcaagag
      361 cgagacgctc gactcgccgc tgctcgagca cagccccgat cccgatgtcg cgctggcgta
      421 cttgggcgat cgcgcggcgg agctgcggat ttgccggatc gtggccgtgc acctcgcgct
      481 gccggacctc gcacatcacc tcgtgatcct ggcgcccgag aagccgctcg cggatctgcc
      541 gcacgcgctc gtcgagggac tgcgccgcga cgtcgagcgg tgtctgcacg accagcaagg
      601 cctcgcggtc atcgtcgatc cgtcagccgt tccggctgca gcagggagtg cagcacatga
      661 gcagtgagag gaaggcgctc gcgaaagatg tcgcgaagga gctcaacgcg cagcggcgca
      721 agcggaagct cgcgacgtac agcgtgttcg gcgcgctgct cgccgccgcg atcttcttcg
      781 cgcgctgcgg gatggggtgg ggcgtcggcg gcgggaaggg cgatggcatc ggcgacggca
      841 acgggaccag caaggcgaag ggcagcggct cggcggcggc gcaggcgcaa cgctgcaccg
      901 tgcgcgtctc ggccgaaggc atcttcgtcg acggcgtgaa gaagacgcgc gacgaggcag
      961 tggccgcgtg caagcagacc gacggcgcga tggtcaccgt gacgggcgac gcgcgacaag
     1021 gcgattggga cgagctgcgc gccgcgctgc aggcggtgcg cgtgaagatc tacattcgcg
     1081 gcgagctctg ggacggcagc aacacgcccg ctggcagcga cgcgccgaac cagccgtgaa
     1141 ccgaatcacg tagctcgatc atcggagcta tatggccgat ctcgatcctg caaacctgct
     1201 cgagattcga atcgaaacga acgacagcgg accatgggcg gaagacgcgg agtgggtgtt
     1261 cgtgatgcgc gacggaaccg agacgcgctt cggcaacggg cagatcacgc tatcggcgat
     1321 tcagtggctg ccgggcttcg acaacgacgc ggtcattcgc gcgatgacct gcaccgacaa
     1381 cgagacgttc atcgtgtggc ggcgcccgga gcaaccgagt aactaggtgc gtcgccacgt
     1441 cgtgacgccg ctgcttcgtg acagcgctac ttcgtcacgc ccttcggaag cagcagccag
     1501 tacttgccgg gaccgcccat gcgtccggcg agctcctcgg cggtcttgtc gtcgccccaa
     1561 tagatatcgg cgcgcacggc acccatgatg ccgccgccgg tgtcctgcgc gatgaggagc
     1621 gagcgatacg gcaccgtgcc ggtcttgccg acttcgggct gacgtccgtc gacccagatc
     1681 ggcgtcgagt gcgcgatgaa tgcgcggtcg atcgcagcgg agcggcgcgg cgtgagaacg
     1741 acgtcttgcg agccggtcgc gcctggctgc ttcgattcct cgaagaagac gtacgacgcg
     1801 ttgaggtcga cgatctcgtc gaagcgcttg gggtggtcga cgaaccactt gcgaatgccg
     1861 ggcatcgtgc cgttgtaggg cgcgacgagc aggttgagat ccttcagcgc cttgccgacg
     1921 cctttgtacg cgcggccgtt cttgccgtcg aagttgagcc agacggtcgt gccgtcatcc
     1981 atgatcgctt tggccgagcc ttcgatgtgc gcgaacagca cgtcgacggg atcgtcggcg
     2041 tacatgagct cgagcttctg gctcgcgagc gcgcctttgc ggatctcgac gcgcgtgtag
     2101 tacggcacga ccttgccgcc gtcgacgaag cggccccaaa tccgccggcc gtggccgtcg
     2161 ttgacgaagt cgtcgagctt gatctcggcg aggtccttcg gccgcgcgag gatcggatac
     2221 tggaacttgc cggccttctt gcgcgacgcg tggagctgct gcacgcaata cccggtcagc
     2281 ttgccgtcga cgccgttctt gccggcggcc tgccaaacga cgaactccgc ctcgaagaat
     2341 gcgcgcgccg ccttgtcgtc gccggccttc accttcgcgg ctgccgcaca ggcatgtcgc
     2401 cactgcttgg cgagcccgcc gtgcccatcg acgccgatcg gcgcgtcgtc ggcgagcttc
     2461 gcgagcttcg cgcacgagtt gagaaacgac ggcagcgctt cgctctgctt gtcgtcggtc
     2521 catccaggca ggtccgtgaa cttcgtcttc gtcagcgtca gctcgtcgtg cggcggcgcc
     2581 tgcactgacg gatcggtatg cgactcgtcg cacgggcact gcgcgacgac gctcgtcgcg
     2641 gaccccgcac ccgaaccaac gcccgagcca gcaccggagc cagcaccgga gccagcctgt
     2701 gccgagtagc agccggcgag gaggacgagc caggacgcgc gcatgcctga cgatgacaac
     2761 gcgcgaaccc gggcgcaaat tcacgcgtct gcacaacgcg ctacgtgcgc tcgcactttt
     2821 gagcttgccg accccgacgt gggtcgctac gttgaggaca tgtcgaacac gctcgcgaat
     2881 cgttgcgctc gaagcgtgct cggccgccag caacgctagt cccgctccgg cgtggtctag
     2941 cggcaggacg cgggactttg gatcctgagg cggtggttcg aatccatccg ccggaacgat
     3001 ccggggtggt ctaactggca ggacggcgag ctctgacctc gcaattcctg gttcgaatcc
     3061 aggccccgga acgatggcga ttccggacat gaaaaaggcc cggcgactct tcgtcgtacc
     3121 gggcctgcgc agcgtcgcgc gctcggcgct actgctggcg agcgatcgtg atcgggcgga
     3181 ggaagacctc gacccagttg tcgtcttcgg gctggttcac aacccacgtc gacatcacga
     3241 tgtccgtcgg cgagagcgcg gccgacgcga agaagccgta cgcgcccggc cacggcgaga
     3301 cctgcgggcc gcccgcgatc gacgtgtgcg tccacttgcc gtcctggcct ttttcggcga
     3361 gcagcatctc ttgcgtcgtc gcgtcttggt agacgacgcg cgggccgtta gcgccggcga
     3421 gcacgaggcc cgcgtcgtcg ccgacgaagt ggtagaccgg cttcggcagg ccgtcgacgg
     3481 tgatgccgtc gatgcggtag ccgtcgtcga tcgtctcctt cggcgcgttc gtcgcgtcgg
     3541 tcgtgaactt caggtcgtcg ctcgtcgcgc cgacgtaagc cacgttgacc acgccgttcg
     3601 cgtcgatgcc gaccgagggc gaccagcccg cgtcgatgcc gttcgtgccg tcgagcacga
     3661 tcgccgtcgc gaattggccc gacgtcgggt tgaacttgct gaccttgagg tcgccgttcg
     3721 tgcggtcgta gtacgtcacg accggtgctt ggttcgtcgg gttacgcgcg gagtcgatga
     3781 agaggccgag gccgcccgga agcgggtaga cctcgtcggg gttcgccgga atgttcgcct
     3841 tgtcgacggt ccagaactgc cagtcggccg acgacgtcgg gttcgcggtt tgcgctgccg
     3901 cgtagcgaac ttccgcgcgc tcacctgcgc cgtctttgac gtgggcgaga tacgcgacgc
     3961 cggggcgacc gtcgtcggag cgcagcgtca gcgacgtgta catgccgacg agcgagccgc
     4021 ccgagcctgg atcgatcttg ctcgtgcctt gctcgacgat atgcgtcgtc cacacgccgc
     4081 cggacttcgt cgcgaacttg agcgacgccg tgtcgacgtc gaagtacgtg accatcggcg
     4141 tgccgtcgga ggccactgcg atcgacgtgt acataccgac gtcggcgccc ttctcctcga
     4201 tgccgccgcg gatcttcgag ccggggatca cgaccgggcc atccggcacg ccgtcgaccc
     4261 actcccacgc ttcgttcggg atgcgaccac cggtcgtctg cgcgacgacg aggtcaccgt
     4321 gcgactccgc gtatgcgctg acccagatcg cgccgccacc gacgccgaca tcgctgtacg
     4381 gtccaatgcg gcccggcggg atgtcatcgg agcagacgca ctcgttgtcg acgcagaacg
     4441 gaagctgacc ttcctcgcag aaatcggggc cgcagtcttc cgcggtctcg caagactggc
     4501 cggcaccctt gccacacgag cacgcgggga tgaacgccga gagcagcgcg atgcacgcga
     4561 cgaagccgag ctgcttggca gccttcttca tcatgcgctt cgcggcgggg gagatgcgga
     4621 gcgaacggcg acggcggcga ccgaacaggc cgagaccgac gaggccgaac agcgcgaggc
     4681 taccggcgct cggcgccttg cccgaatcgc agctgcagcc cgatgcgccc gcctggccgt
     4741 ggaacggcgc gacgtacgcg atcgtctggt tgccctgctg gtcgcgcgcg aacacggcga
     4801 tgtcaccgtt gccgagatcc gcgaggtgct tcgccgtgat gagcgcgagc gtcgcttcgc
     4861 gagccggcac ccacttcgtc tgcggcgcgt catcgccgac cgcgccgaac gcgatctcga
     4921 tgtccttgcc gtcgacgacg tcccagacgt ggatcttcat ctgatcgccg tcccactccg
     4981 tcttgtcctc gaagacgcgc gggccaaccg agtcgatcac gaccgccgtg cgaatctcgt
     5041 cggagacggt gtggtagtcg cccttcacgc gcgacttgag accgatctcg tacttgccct
     5101 gccacgcgaa cgcgcggtcg ctgatgatca gctggccgcc cggctggtac ggacgataaa
     5161 ggccgccgtt gatgttccac gtccactcga gctcgcggcc catcgtgtcg tagcgatcga
     5221 cgtcgaacgt gacgctcggc agctggccgt cttggccggg aacgagcgcg ccgcgaatga
     5281 tttcagccgg cggcgtatcg accgcgagca gacgagcctt gcccgtgctc tgcaccttca
     5341 gcgaaggcgc gtcttcgtcg atcagatcga ccgcgtgccg catgaacggg tcgttgtcgg
     5401 cagcgacgcg catcatcggg ctcgagccga gcgacgcgta gagcgcgagg aactcgtcct
     5461 ggctcgtcgt caccttctgg atcgacagat tgttcagcga gaagcccgcg aacgacggca
     5521 cctggatcgg aggcagctga ccgagcagcg gcgtcacgag atcgaacacg gacggcagca
     5581 cgccctcgag gtgttgcggc gtctcgcgca cgaactggct gttgagcact ttgacctgca
     5641 cctcgctcga cgagatgccg acgagctcgg gcttgatgac ggcgggcccg ctggccggct
     5701 gctcgaacgt catgttgatg ccgacgttca tcgtcagctc gagcgtgaac gcgcggacgt
     5761 agcgctcgta gaggaacgcg tagaagtcga cttcgatgtt gcggaggtga atcgtcagat
     5821 tcggatccgt catcgtgcca gtaccgatcg agaagtcgac ggccttctgc gggcgcgtca
     5881 cgagcagcag tggatcgttg ccttcatcgc tagcgagctc gccgagcgac ggaacaagaa
     5941 tgccgatcgt gccgacgttg agctgcttga tgtagctcgt gccgacgccg aggcacatgc
     6001 cgccgctcgt cacgaggtgg tgaccggtga ggttcagcgt cgtcttcgag agacccatcg
     6061 cgatatccgc ggcgggatcc gggttaccgg agaactgacc ggccggcgcg agctggaacg
     6121 tgttgccacc gaggcccgag cgcgccaccg tcggaagcga gaacaccggc gccggcatcg
     6181 gcgggacgca cagcgcgggc tcgctatcga gcgcgaccgt acgcgtcgcc ggatcttcgt
     6241 cggcgttcat gcccgtgatg acgcccatgt tcatgccgcc gttcgccgtc atcccgacgt
     6301 agccgccggg gacgatgcgc gcttccatga agccgtccgt gccgggcgag atgccctcga
     6361 gcagcgagcc gatgttgacc atgcccttca cgccgagcgg attcggaatg aagctctgaa
     6421 tgagcgggtt cagcagcggc gtgaggaatc ccttgatggt gttcgggatc gcgtcgatga
     6481 gcgtcgtgag cagctgaccg atatcggaga tgattccgca gcccgaccac tgcaagttcg
     6541 tcgtgaacga gttgatgttc gcgagcttga tgtcgagctc gccatcggca ggcttgatgc
     6601 cgaagccaac gtcgaagcta ccgttgaggt tgctcgactg caggcgcatg ttgcacgagt
     6661 cgacggtgac gccgaacacg acgaagtcga tcgggatcgt cgtgtcgatc ttcgtcgaca
     6721 gattcacgcg aaggttgttg ccgacgactt gcgtcgtcag accgttcggc gtgttgacct
     6781 gcgcggtgat cttgcagccg cccgagcaac cgccgccgct ctggcagtac ttcgccgtcg
     6841 cgatgtcgaa gttgaagagg ctgacgcctg cgcttccctt cggcagacag aagccgctgg
     6901 acagctgctg attcagcaag ccaggcaaga tcgacgagag cttcgagaag ccctgtggtg
     6961 tgacgcgaac ctgcgcacca ccctcgacgg tttgatccgt cggcaatttg ccgttcggca
     7021 gtggacctgt cgatccacat gctccgcagc taccaaggtt tccgcatgct cccatcgtga
     7081 tgatgaagag cagcgacgca aaatgattca cgcgcgcttt acgaacaaac actgcaatcc
     7141 tcccgggcag gaaactgcac cctcatcgtg ccgatcctgc ggtgacgtat acctgaaggc
     7201 cgtttcggat tggaagtgca tttattacgt agacgcgcgt caattctcag gttttccggc
     7261 ctgaccaggg atcaggtact tggggtagga aaatgtgagc tgccgccccg cgcctacaag
     7321 tgggctgagc aagtgcgcgc cctcgaacgg acttgacagg tacggcttcc gaccgctatc
     7381 tactgccgcc caacaagggt ccgtagctca gttggataga gcgccgggct tcgaacccgt
     7441 aggtcgggcg ttcgagtcgc cccggactca ctgacaatag aagacgtagc gatcaccgcg
     7501 atgcggtggt cacccaaccg ggggcatagc tcaatggtag agcatcggac tcttaatccg
     7561 ctggttgaag gttcgagtcc ttctgccctc accccgacac tcgaaaacgg ccacctcgaa
     7621 gcaggtggcc gttttctcgt ttcggtcgtc aggtcttctg tttttatcgc aagcctcccg
     7681 atcgggaaga ttcgcccagg gaggctcgcg tcatcccgat cgggatggaa agcttcagcc
     7741 gccgtggtat cttcccgatc gggaaggaaa cgatggctac gaagcccaag acaacgccga
     7801 tgcgggtcaa gagcgcgacg gacctcggat ctgtgattcg cgatgcgcga aagcggagcg
     7861 agctcgatca agagacgacg gccgacttga tcggcgtcgg cccgcgcttt ctcagcgaag
     7921 tcgagcgagg aaagccgagc gcacgcctcg ggctcgttct caaggtgctc gaacggcttg
     7981 gcctcgaggt ttgggtttcg ccgcgcggct ggaagccgtg agcgcgccgt tggaggttcg
     8041 gtatgaggac cgacgcgtcg gcatcctgcg cgctgacgcg tcgaaccgtt tttgttttcg
     8101 ctacgtcgag gagtggctgg gtgcaaggga tgcattcccg gtctcgattg cgttgccgct
     8161 gggcccgcac gaggagagcg gcggaagcgc gcactcgttc ttcgcgaacc tgcttcctga
     8221 aggtttagtc cgtcaagccg tatgccgtcg cctcggaatc tcggagagca atgacgtcga
     8281 gctccttcgc gcgattggcg gcgagtgcgc aggcgcgctg tcgatcgtgg aggcgggcgc
     8341 caagctgcaa gcacccgaca acgagtatga ggagctcgac gcgcaaaagc tccagtcgtt
     8401 ggcggatgac gacgatcgtg tggtgccgtt gctcgtgggt ggtgcgcaga cgcgactctc
     8461 gctcgctggt gcacaggaga agcttccggt cgcgcttctc gacggcaaga tccatctccc
     8521 actgaagaga tcggcgagca cgcacatcct caagctgccg aatccgcgct acgcgcatct
     8581 gccgcagaac gaggcgttcg tgctcggcct cgcaagccat gcaggcctgg aatgcgcgag
     8641 ctcggagcta acgagagcaa cgaaaccgca gagcctgctg gttgaacgtt acgaccgaag
     8701 gcgaaccgac gatccgtggc ccgtccggcg cctgcatcaa gaggatttct gtcaggcact
     8761 cggattgctg ccggggcaaa aatacgaaca ggagggcggc ccctcgctcg cgcaagcggt
     8821 ggctgtcgta cgcacgagcg tcgcgaatcc gctccacgat gttcggcgcc ttatcgaatg
     8881 gcaggcgttc aatgcagcgg tgggcaattc ggatgggcac gggaagaacc tgtcgatcct
     8941 ctacgacgac gaaggcccgc gacttgcgcc gttctacgat ctcgtgtcga cgcgagaata
     9001 cgcgaatctc gatcgcctat tggcaatgag cgttggtggc cggcgcaacg ccgacgagct
     9061 tggctacgag cagtgggtgt ccctcgcgtc cgagctcgac atgagagatc gcgtggtcct
     9121 cgacatcgtc ggccgcaccc tcgaaaccgt tgccgacatt cttccgcgct ggacggacac
     9181 atttcgagag cggtacggac gcttgcctgt cctgcagaca cttccaggcc gcatcgacaa
     9241 ccagctccgt cgtttgcgtc gaggaatcga gaagcccgcc aagacggcga atgcgcgcaa
     9301 ggtcaagggc ggaaagacca agaagcgctg agactgcgaa cccggaaatg tcggcgatgc
     9361 ggcgttgcca cgagcgacgt ccccggcctc taattgcgcc atgggaagcg cggagtttct
     9421 ctcgctggtg ccgctgtttc tcgcggcgct cgtcccggcg tgtttgctcg tgtcgtcgtt
     9481 tacgatctcg aggcagcgcg cacgctcgct gcccgtggcg tcgttcgtca cgacgatgac
     9541 ggcggtgatc acgtatctcg ccgtcttctt gcgcgtgctc ttgtgggcga ttgggttcga
     9601 cgcacgcgag gtgattccga catcggtcat gcacgacgtc gtgcagatcg acatcgtgtc
     9661 gggcgcgatg ttcgtgctcg tctcgacgct cgcgatgatc gtcgtccggt actcgaggac
     9721 gtacctcgct gggcaggcag agctcgaccg gtacgcgcgg tatctgttgt tgacggtcgc
     9781 gtcggtcacc gtgctcgtga cgtcgaacca cctcgccgtg ctcatggtgg cgtggtttgc
     9841 gaccgacatc ggtttgcacc agctgctgac gttttatcgg cggaggcggc aggcgatcat
     9901 cgtcgcgcac aagaagtttt tgctgagtcg cgttgccgat gcgtgcttcg ttggatcgct
     9961 cgtgatgatc ggtggcgctg ttgggacgct gcgcatcgac gtcgtgaata cgttcgcgcg
    10021 gtcgcagggc gagctgtcac cggcgctgca tctcgcgacc gtgctgctcg tgattggcgt
    10081 gatcttgaag tcggcgcagc tgccctttca tggctggatg ctgcaggtca tggaggcgcc
    10141 gacgccggtg tctgcgctct tgcatgccgg cgtcgtgaac atgggcgggt tcgtgatgat
    10201 tcggctggcg ccgctgatgg cgcacgcgtc gctcgcgcag aatattttga tcggcgtcgg
    10261 gctgttgtcg acgatcgtcg cgtcgctcgt catggtcacg cgtgctgcga tcaagagctc
    10321 gcttgcgtgg tcgacgatcg ggcagatggg cttcatgctc gtgcagtgcg ggctcggcgc
    10381 gtggcacttg gcgctcatgc acgtgctcgc gcactcgctg tataaggcgc acacgttctt
    10441 gagctcgggc tcgatcgtga ggcagtggcg cggcgcgcac ctcgtgcata gcccgcatgc
    10501 gctgttgtgg catttgatca tcgggacgac cgtggtgtgt gcggcggttg cgccgttcta
    10561 ttacgcgact gcgctgccga ccggacacct cgcgagcacc acggggtcac tcgcgttcgt
    10621 gctcgcgctc agcttcgtgc cgatgatcgg acgggcgatc gcagcgggga agcgggcgtt
    10681 cgtgctcgca gcgttgctga cggtgggggc gtcagccgcg tacttcgcgt tccatgtcgt
    10741 ctttgatttg gtcgcaccgt cgatcatgga aacgcccccg tcggagctga agtggcagat
    10801 cgtcgtcggc gggctcatct tcttgttcgt cgcgcagtcg atcatgcaga cgagccccaa
    10861 cgggcggttt gccaacttca tccagcctca cctcgacagc ggcctttaca tcgatgactg
    10921 gttcacacgc gtgacgttcc gcttgtggcc accggcactc caatgagcga cttggttgga
    10981 cttcgcgaag atgccgagcg cgcgtgtgcg cggatcgcgc cgacgtggcc gctcgaccgg
    11041 ttcatcgcgg tcaatccgtt ttggccgctc acgaacaagc ccgtgcccgc ggtggccggt
    11101 gatctggcgg cgttgtcggg agcgcggttg ctcatgccgc gcgagtggta cgcgcaggag
    11161 tggcgagcgg ggcggtttcg ctccgagcac ttgaaggaag cgatcgcgga gagcggctcg
    11221 aatgcgacgg aggacgatct catcgcgagc ttctggatcc gcgagcagga gccgcttcgt
    11281 aggccgctcg tcgtggacgt gatggactcg ctgctgcggc gcgagcacga gctgacgtgg
    11341 cgcgacttcg tgatggagcg catcagtcgg ttctgcgcgt cgtacttcga tgacggacaa
    11401 gcgcagatca acggcgtgcg cgagggtggc ttgtacgcga gctggctgcc gcaggccaag
    11461 atggatcagc tgccgaatct gttcatgaag ctgaacgggt atcggtcgac ggtcgcgaag
    11521 ctgccgaaca ccgcggacga gatgatcgcg cttggctgcg cggatctcgg cgtgcccgtc
    11581 gcacaacgcg agcggtatct ggccgcgctg ctgctcgacg tcaatgggtg ggcatcgtgg
    11641 tgtgcgtacc tgcggtttac ggcacgccag gggagtggcg acgacgtgca cattcgcgag
    11701 ctcctcgcga ttcgcctcgc gtgggaatgg atcttgttcc tcgcgagcga cgacagcctc
    11761 cgcgccgagt ggcggttcgc gatgacgagc tggcccgcga tcgatcgcgc cgcgcgcgcg
    11821 agccgcacgg gatggacgct gcaacgtgcg gtcgagattg catggatctc gcaggtgcgg
    11881 tcgaagctgc tgcaagggtt cgacgccaag ccgatcgcgc agccgaagct gcaagctgcg
    11941 ttctgtctcg acgtccgctc ggaagtgttt cggcgcgggc tcgaagcgct cggccaggat
    12001 attcagacgc tcggcgtcgc cggcttcttc ggcgtcccga tcgagtacgt gccgctcgcc
    12061 gcggatcacg gctcgcctca cctcccgggc gtgctcgcgc cgaagtaccg cgtgacggat
    12121 acgggcgtcg agagtgggct cgagactacg cgtcgcgcgc ggctcgatgc ggcgtatgcg
    12181 tggaaggcat tcaagtcgag ctcgctctcg agctttgcgt acgtcgatgc gataggcctg
    12241 ttcttcgcgc gcaagctgtt ccgcgacgca tggcgtcccg agcgttatcg cgacgacgag
    12301 aaagagcgtc cgggactgac cgaagacgaa gacgcgaagc gcgtaccgcg gctcacgagt
    12361 cgcgtcgacg ggacggcgct ctccgccgac gagcgttgcg acatcgccgc gggtctgctg
    12421 aaggcgatgg gcctcacgcg caacttcgcg cgcctcgtgt tgctcgtcgg acacggcagc
    12481 gagacgagga acaacgcgta cgcagcgggc atcgattgcg gcgcgtgtgg cggtcatccc
    12541 ggtgacatca acgcgcgtgc cgcggccgcg ctgctcaacg acggcccgat ccgctcgggg
    12601 cttgccgcgc gcggcatcga gattcccgcg tggacgttct tcgtcgcagc acttcacaac
    12661 acgacgaccg acgaagtgac gctgttcgac gtcagtgccg ccgcgcagac gcatcagaca
    12721 gatctcgaca acgtccgtgc ggcgctcgaa cgtgcgggct tcgctgcgcg acgcgaacgt
    12781 gcaccgcggc tcgggctcgc cgagctgacc gacgcaaagc tctatgccgc cgtcgtcaag
    12841 cgggcgacga actgggcgga agtgcggccc gagtggggcc ttgcagggaa cgccggcttc
    12901 atcatcgcgc cacgcgagcg cacgcggcac atgaacctcg aagggcgcgt gttcctccac
    12961 gactatcgcg tcgaggacga ccgcgactac tcgatcctag aagcgatcat gaccggcccg
    13021 atggtcgtcg cgcactggat caacttccag tactacgcgt ccaccgtcga caacgcgcgc
    13081 tatggcagcg gcaacaaggt gctccacaac atcgtcggcg gccacctcgg cgtgttcgag
    13141 ggcaacggcg gcgatctgcg aatcggcctg tcgctgcagt cgctcaacga tggtgagcac
    13201 tgggtccaca cgcctctgcg gttggccgtc ttcatcgagg cgccgcgaga agcgatcgat
    13261 tccgtgctcg agaagcacgc gaaggtgaag gagctcgtcg acaacgagtg gctgcacctg
    13321 taccagctcg atgtgaccga gcgagctctg tacgcgcggc gcggcgggac ctggaaaccg
    13381 gctccgggct gacatcttcg agtaacaact cgtggtgatt gggggcgtgg gcaccgcgcg
    13441 ggtgagccgc cccaccggca tttcgcgcgg cccgcgtgca tatccagtga cttagacctg
    13501 gccgaggcct tgctctatct gcgggcatgc ggcgcttgct cctcctcgta gtgatcgcgg
    13561 cgtgcgggac gacagaggac gaggtgccga cgtacggcga ggggctcgga actcccgaca
    13621 gcccgatccc gcaggacggc gcgccctaca cggtgcacac gcagacagcc gcgccgagca
    13681 cgacgacgca agcggaggtc acgcggatcg cgggcgcgct gcgccagttc tcgcagacgc
    13741 cagcgcgctc gatgctgacg ctcgcgaagc aagcatcacc cgaggagctc gcgacgctcg
    13801 atgcgatgtc atcgacgctg cgctcgcggc tcgaaggttg gatcgatacc gagatcgaca
    13861 aagtcgtcgt cgacggtgtg cgcggtcgcg cggtggcgtc ggagatggcc gacttcgtcg
    13921 actcgacgct cgcgcacttc tcgttcgaga gcacgctgtc gttcacgccg acgaagacga
    13981 cgcacacgct cggcgcagtc tcgttccggc tctcgggcct cgacgtcgtt gtccccgtcg
    14041 gcgggctcgc gggtgatgat gcgcagcagc gcgtctcggc caccgtcgga gaaggcggct
    14101 cgctcgcgtt cggcgatcac acgttcggca tcgatttcgg cgcgcacgcg tggcacggca
    14161 tcaacctcgc gagctcgaca ctgcacggtg tcggcatggc gcctgcgctg tcgaacgcag
    14221 tcaactgcaa ggcgctcgcg caggccgttt cggccaagtg ctacaacggc gcgtgcgtcg
    14281 ggcactcggc ggagatcacg tcgc
//
LOCUS       DATLBZ010000459        12765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9517322, whole genome shotgun sequence.
ACCESSION   DATLBZ010000459 DATLBZ010000000
VERSION     DATLBZ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9517322"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VMZ53_23140"
     CDS             complement(<1..327)
                     /locus_tag="VMZ53_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31429.1"
                     /translation="MTQQPDYFANHEKGRKFPWTLYHAPLERDLSQFLSLVSAWKHRL
                     QQADGDVLVVGCGLLHEIDEAPEGLRFTVVDIDPRAVDAVLARKDPRIIAGHVVPPET
                     PLDQVLG"
     gene            complement(344..1249)
                     /locus_tag="VMZ53_23145"
     CDS             complement(344..1249)
                     /locus_tag="VMZ53_23145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS31430.1"
                     /translation="MKGLMLKAVPDLPPDEMNAPSDDDVLRWALAYLEHAPLSLTLRE
                     INRLVALETVTTKRGPVLDVGCGDGFWWTLRSDKREIYGIDISASEIAQARKRINAQL
                     SDVSRELPFPGTKFGELIGNCSLEHVPDIDAALANLRAAASDDAQLLMFVPTPRWAYQ
                     GRMQSFLLRYSPRMAMALSGALNGFFQHWHLYDHKVWTRLLETHGWRVREIKGLGSAR
                     SEFLFRAFMPPAFVQFLAKSITGFYPAKLYRFLPKAAMKPLAKLVRWAVSDPIVDVES
                     PHAYEYMIVAEAAPSSSSSGAASPK"
     gene            complement(1206..2813)
                     /locus_tag="VMZ53_23150"
     CDS             complement(1206..2813)
                     /locus_tag="VMZ53_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31431.1"
                     /translation="MTRRWTIAIFVATFGVYMLASSREPAWGDSHSMWEVADRLVEHG
                     KIDIKTRWPEDIPPGRGGKIYSINPIGVSLVHVPGAAGAALTHRWMPEKDVLLRPIFT
                     HVGPAAMGALACVMFFLLLVDLGRSKRTASLCTAILALATTTFVYARMPYSEILQLAM
                     FMGFFRQTLKLASEPTRREALWWGAWAGLLLNAKYVFALAIAGGAVLVAWALRSKRAE
                     LVRLTGWAAVTGAPLLVLAAVYNYLRWSDVTASGYGPYLSAFFGGSVFDGAWGMLASP
                     NKSAFLYSPPLLLALLGWPAAIREHRRLGLALLVVVLPTFVVYCTYRSWSGDYAWGPR
                     FFVWAVPVLLVGIAWFVDRMSRWRGAFVGLVVACGIAVQLVGSALYWDHFIRIAIDVK
                     NQWLGNPNRSGSYIAERGRGHCDSCFEDTYEIMWTPAFQPIEGNWWLVKSIARGDDDW
                     HIAQQDAPWRTYTHLEMNLAATYPRARIDWWGLLWIKDAPKLRPLGIFLLLLFAAISG
                     FGIWRWIRLHRSGNEGIDAEGRAGPAS"
     gene            complement(2824..3699)
                     /locus_tag="VMZ53_23155"
     CDS             complement(2824..3699)
                     /locus_tag="VMZ53_23155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31432.1"
                     /translation="MRPWLVLLVTLGVSGAVGYKALRAAGAFDPVAEVPHRHREYATT
                     TDAMTAILAENPVPRVYAIGEYHQTRDAIAATSPLARFTHEIIGMLEPHAQHLVIESW
                     LDANCWGDKRDPVAAMLNKPNGVSMDVMRLVSRTKKLHMQAHTLPMTCIEYDSVVDFS
                     GHVNFLLLLDLITEKLAETTRGLLRSGDGTSVIVYGGALHNDLYPRWPLETLSYARPL
                     ADSMGGHVLEIDLVVPEVVAPMEMIRTEPWFPLLARSSPSRVIVWNRGPGSYVVILPA
                     QSEAAGRVAKLVDPM"
     gene            complement(3739..4638)
                     /locus_tag="VMZ53_23160"
     CDS             complement(3739..4638)
                     /locus_tag="VMZ53_23160"
                     /inference="COORDINATES: protein motif:HMM:NF021554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HUS31433.1"
                     /translation="MFGPSRSADSTRTDHRLESGLAVAGLYAGFSAWAYIAWYRNHPE
                     KDEHDFGRDGWFGKHTYAGGADKLGHAWATMVLARGGTAALRAGGWDRTKSALVSATL
                     SEGLFFAVEVKDYFYYEFSPGDFTFNTLGALTALALDLSPRLDELFDYRVEYWFSDQY
                     LHNLFDSDSPCAKRVPGQPSCSRWNIAEDYSAEKYLIALHLGAIRPIRDLSKWSRFVD
                     VDVGFYSRNYKPPPTLEPNALKTQTLFLGVSLNAQGVVDALLPHESKLRKTGHGFFEV
                     VGPPMTIGVGAQRSTSRVNTGGA"
     gene            5083..6384
                     /locus_tag="VMZ53_23165"
     CDS             5083..6384
                     /locus_tag="VMZ53_23165"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUS31434.1"
                     /translation="MKLLALVVFINRYVAGVLIRILRGKKWDETRDDFEPTVTVVIPM
                     FNEGEAIKETLQSLLDSDYPSHKLQIVCVDDCSTDNSFEIAREMARASGGKLKVIRNR
                     VNLGKRRSINRAVRSAESEIIVSVDSDVVVDANAIRELTRRFTDDRIAAVGGWVDVRN
                     KHDNWLTRMQVIKYWYGYHFLKNLEWGFRRVMCLSGCLTAYRRSVLVELEPVLEDRTI
                     LGVSIKYGEDRFLTRQIIKAGYLTTMTHAARCRTFVPNTLASYFSQQLRWRRSNIVDY
                     SGAVSHVWRLNPFIAIHFFAQFALLVAYPIAVVRALSSGWFFNGLQIHLYVITLMGLY
                     YWFRVRKLPKEERVGVLSFLPIALTMPVTYALLTPLALFTLDSGSWETRGHEAEPDAA
                     DEPVTDIHTGQHILVPALATEPVRSRAVDRHTGSQAQLPAA"
     gene            6449..7246
                     /locus_tag="VMZ53_23170"
     CDS             6449..7246
                     /locus_tag="VMZ53_23170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31435.1"
                     /translation="MEAPRKTPKFWMAVQPSIIRLYKVAGLVALTAILVGLLGFLVVN
                     IFYFFDNSWVRPVVLSPNHPKVVEASNLVADAKLRQSQLDTEQMEITAELGEIDRAVA
                     ADDKFITDVGTQADAPKSTDQLMLRREVDKAKLDKENQQGRRAPLKARLETLSLRIKD
                     QEQVVHRLEASPYLRAVNGKVVLAFVPYTNLKHVSIGTKLYGCSWGLVMCSRVGKVKA
                     TIDGEVQDIHPHDQSVQRGIMVEIELSTPSAEGNTVLFAGGKPLWLF"
     gene            7375..10638
                     /locus_tag="VMZ53_23175"
     CDS             7375..10638
                     /locus_tag="VMZ53_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31436.1"
                     /translation="MSDDNQGEEKKTIIGQGPSAGRYAVSKPGDRTVVIPNAAAAAPR
                     KSPTMPGVNVTPEPQKRPPMVVMPPSAATVVMAPQQPTPAPPVVATGTPLPQPVANPT
                     MASSGRARRSSEAPSGVMPRSAWDSPSGISGISPGSTPGTPPSVGGSDGATGVHATAS
                     KPHPGVRINQYELIKLIGEGGMGQVYVARDLRLGRRVAIKFLLSETHPELTQRFLVEA
                     RATARCQHENIVTIYEVGEHVSAPYMVLEFLSGKPLTAQIKNSGKLPYTRAVEVMTHT
                     LKALECAHGQGIVHRDLKPDNIFITDAGNIKVLDFGIAKVLEASPQNAVQTSAAGIRL
                     PSPLELATGSNTSLTRHGTIMGTLKYMSPEQWGIGIEIDHLSDIWACGILMHRMITGK
                     HPLAPLDGNQLVVTAMLELPMPSMAESAPPDCPKELIQIVDKCLYKIKEQRWQSVSEL
                     LRALEPFMPGRRTQTLQIDESPYAGLSSFQENDAGKFFGRNREIAAMVTRIRDRPLMG
                     VVGSSGVGKSSFVRAGLVPALKRSGEQWETLVTRPGRSPMDALAVMVAPMVGTSSNLA
                     DELNEQTKLAARLRKEPGHLGHLLRGRARKESRRIMLFVDQFEELYTNVEDPAERAAF
                     TAALAAVADDATSPLRVVLSIRSDFLDRCAEDQTFLADLTQGLFFLNQPNRDSLREAI
                     TQPAELAGYSFEVPATVEDMLDHLETTPGALPLLQFAASKLWEGRDVARKMLTHNAYA
                     AMGGVAGALASHADRVVQELGQSNAPLVRAILLRLVSPERTRSIVPLDDLRELSREVG
                     AVQRLVDQMVDARLLVVQTLEGGKGSTVEIVHESLINNWPTLRRWLDETQEDAALIDQ
                     LRTAARQWNAKGRSPDLLWRGDTADEAKKFRRRHKGPLSDIEQSFLDEIIAYEGAVAR
                     RKRVAVIGAFVGLGILVIAAMVALVIIQKSRTEAKDQAVIAEQNRKEAEENLAAMRAK
                     EAERQKAEAEKAKAEQKVVVSEVKLDAAQVDLAKKNQELEAAVVVARENEAEAKRAEA
                     RAKAAQKEAEQAQGEAVAAKDQVAIQKMQVELLLKQEQERVKRLQSQIGSPIVDDLK"
     gene            10659..11756
                     /locus_tag="VMZ53_23180"
     CDS             10659..11756
                     /locus_tag="VMZ53_23180"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS31437.1"
                     /translation="MTKAIWGLALGLGIVLSLRTAHAQAPQPETPPKPEKTPKSDEKA
                     DTTETLQKGGDVRPWAQGVPPAEQKTALLAFREGNSQLNDGLFAKASETYRGALTHWK
                     HPAIYYNLSLALMNLDQPVEAYESMQAATTFGEGPIDKDKFEHAKEYLLLLGKQIATI
                     TVACDKPGAKVSLDGKEVFIAPGKHTQRVRIGRHTLVAEKTGYQARVNAPNIGPGETF
                     SVELKLFTADELTRYRRRWETTWMPYAVLGAGIGVGLIGVGLELGADSSYNDYDKKVA
                     ACSMGNTGCENTQALKDLRSRGDTMKTLGYVSYGVAGATIAGGIVLAILNRPKSYLLR
                     PEEVQDQLDQKVTFAPIVTPTMAGASVQGHF"
     gene            11756..>12765
                     /locus_tag="VMZ53_23185"
     CDS             11756..>12765
                     /locus_tag="VMZ53_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HUS31438.1"
                     /translation="MKYLLLALFITACASTPDSQICERSGIVCPEGTKCAAVQAVCIT
                     NDCGDGITAGGETCDDGNIIDGDGCSADCRSKESCGDGILSAAGGELCDDGNDDDCDG
                     CSHDCKSIELCGNDIKDCGEACDDGNTVGGDGCASNCKSTESCGNGVVDTQVGEVCDP
                     PGANCGVDCRSLLACGNGIVDAGEQCDHGQANNGDDKDCTHDCKINVCGDGLINLVGP
                     DHKEQCDGGTTGSPKETNLCNLDCTNAVCGDNKINQTAGEQCDSTSGNDSPSCNGQNG
                     GAVKCKLSSCGDNYINPAAGETCDITGGADSPSCNGSSAGQKACKAAVCGDNFLNSQA
                     GET"
BASE COUNT         2437 a         4184 c         4085 g         2059 t
ORIGIN      
        1 gccgagaacc tgatcgagcg gcgtttcggg cggtacgaca tggcccgcga tgatccgtgg
       61 atcttttcgc gcgagcactg catcgaccgc acgcggatcg atatcgacca ccgtgaagcg
      121 cagcccctcg ggtgcttcgt cgatctcgtg gagcaggccg caaccgacga caagcacatc
      181 gccatctgct tgctgcaagc ggtgcttcca cgccgacacg agcgagagaa actggctaag
      241 gtcgcgctcg agcggcgcgt ggtagagcgt ccacggaaac ttgcggccct tctcgtggtt
      301 cgcgaagtaa tcgggctgct gcgtcatcgg cgactctcga cgactacttc ggcgaagcgg
      361 cgcccgacga cgacgacgac ggcgctgcct cggcgacgat catgtattcg tacgcgtgcg
      421 gcgattcgac gtcgacgatc ggatccgaga cggcccagcg cacgagcttc gcgagcggct
      481 tcatcgccgc cttgggcagg aagcgataga gctttgcggg gtagaagccg gtgatcgact
      541 tcgcgaggaa ctgcacgaac gccggcggca tgaacgcgcg gaacaggaat tcggagcgcg
      601 cggagccgag gcctttgatc tcgcgcacgc gccagccatg cgtctcgagc aggcgcgtcc
      661 acaccttgtg atcgtagagg tgccagtgct ggaagaagcc gttgagcgcg cccgacagcg
      721 ccatcgccat gcgcggcgag tagcgcagca ggaacgactg catgcggcct tggtacgccc
      781 agcgcggcgt cgggacgaac atgagcagct gcgcgtcgtc ggaggcggcg gcgcggaggt
      841 ttgcgagcgc ggcgtcgatg tcggggacgt gctcgagcga gcagttgccg atcagctcgc
      901 cgaacttcgt gccggggaac ggcagctcgc gcgagacgtc ggagagctgc gcgttgatgc
      961 gcttgcgcgc ttgcgcgatc tcgctcgccg agatgtcgat gccgtagatc tcgcgcttgt
     1021 ccgagcgtag cgtccaccaa aagccatcgc cgcatccgac gtcgaggacg gggccgcgct
     1081 tggtggtgac ggtctccagc gcgacgagac ggttgatctc gcgcagcgtg agcgagagcg
     1141 gcgcgtgctc gagataggcg agcgcccacc gcaggacgtc gtcgtcactc ggcgcgttca
     1201 tctcgtcagg aggcaggtcc ggcacggcct tcagcatcaa tcccttcatt acctgatcga
     1261 tggaggcgaa tccaccgcca gattccgaag ccgctgatcg cggcgaacag gagcagcagg
     1321 aagatcccga gaggccgcag ctttggcgcg tctttgatcc agagcaggcc ccaccaatcg
     1381 atgcgagcgc ggggatacgt cgcggcgaga ttcatctcga gatgcgtgta cgtccgccag
     1441 ggagcatcct gctgcgcgat gtgccaatcg tcgtcgccgc gcgcgatcga tttgacgagc
     1501 caccagttgc cctcgatcgg ctggaacgcg ggcgtccaca tgatctcgta cgtgtcctcg
     1561 aagcacgaat cgcagtggcc gcgaccgcgc tcggcgatgt agctgcccga gcgattcgga
     1621 ttgccgagcc attggttctt cacatcgatc gcgatgcgaa tgaagtgatc ccaatagagc
     1681 gcgctgccga cgagctgaac cgcaatgccg cacgcgacga caagcccgac gaacgcgccg
     1741 cgccatcgcg acatgcgatc gacgaaccac gcaattccga cgaggagcac cggcacggcc
     1801 cacacgaaga agcgcggtcc ccacgcgtag tcgccgctcc acgagcgata cgtgcagtag
     1861 acgacgaacg tgggcagcac gacgacgagc agcgcgagac cgagccggcg atgctcgcgg
     1921 atcgccgcgg gccagccgag caatgcgagc agcagcggtg gcgaatagag aaacgcgctc
     1981 ttgttcggcg acgcgagcat gccccacgca ccgtcgaaga cactgccgcc gaagaacgcc
     2041 gacaagtacg gcccgtagcc gctcgccgtg acgtcgctcc accgcaggta gttgtagacg
     2101 gcggcgagca cgagcagtgg cgcaccggta acggctgccc atccggtcag tcgcaccagc
     2161 tccgcccgct tcgatcgcaa tgcccacgcg acgagcactg cacctccggc gatcgcgaga
     2221 gcgaagacgt acttcgcatt gagcaagagg cccgcccacg cgccccacca cagcgcctcg
     2281 cgccgcgtcg gctcgctcgc cagcttcagc gtctgccgga agaagcccat gaacatcgcg
     2341 agctgcagga tctcggagta cggcatgcgc gcgtacacga acgtcgtcgt cgcgagcgcg
     2401 aggatcgcgg tgcacaggct cgcggttcgc ttcgagcgcc cgagatcgac gagcagcaag
     2461 aagaacatca cgcatgcgag cgcgcccatc gccgccggcc ccacgtgcgt gaagatcggc
     2521 cgcagcaaca cgtctttctc gggcatccag cgatgcgtga gcgccgcgcc cgccgcgccc
     2581 ggcacgtgga cgagcgacac gccgatcgga ttgatgctgt agatcttgcc cccgcggccg
     2641 ggcggaatgt cctcgggcca gcgcgtcttg atgtcgatct tgccgtgctc gacgaggcga
     2701 tcggcgactt cccacatcga gtgcgaatcg ccccacgcgg gctcgcggct cgatgccagc
     2761 atgtacacgc cgaacgtcgc gacgaagatc gcgatggtcc agcggcgcgt cacacccaat
     2821 gcgttacatc ggatccacga gcttcgccac tctaccggcc gcctcgctct gcgccggcag
     2881 gatcacgacg tagctgccgg gaccgcggtt ccacacgatc accctcgacg gcgacgaccg
     2941 ggcgagcagc ggaaaccacg gctcggtccg gatcatctcc atgggcgcga cgacctccgg
     3001 cacgacgaga tcgatctcga gtacgtgccc gcccatcgag tcggcgagcg gccgcgcata
     3061 gctgagcgtc tcgagcggcc agcgcggata gagatcgttg tgaagcgcgc cgccatacac
     3121 gatcacgctc gtgccatcgc cagaccgcag caggccgcgc gtcgtctcgg cgagcttctc
     3181 ggtgatgaga tcgaggagca gcaggaagtt cacgtggccg gagaaatcga cgaccgagtc
     3241 gtactcgatg caggtcatcg gcaacgtgtg cgcctgcatg tggagcttct tcgtcctcga
     3301 aacgagccgc atcacgtcca tgctcacgcc gtttggcttg ttcaacatcg ccgcgaccgg
     3361 atcgcgcttg tcgccccagc agttcgcgtc gagccacgac tcgatcacga ggtgctgcgc
     3421 gtgcggctcg agcatcccga tgatctcgtg cgtgaaccgg gcgagcggcg acgtcgctgc
     3481 gatcgcgtca cgcgtctggt ggtactcgcc gatcgcgtag acacgcggga ccgggttctc
     3541 ggcgaggatc gcggtcatcg cgtcggtcgt cgtcgcgtac tcgcgatggc gatgcggcac
     3601 ctcggctacc gggtcgaacg cacccgcggc gcgcagcgcc ttgtagccga cggctccgga
     3661 cacaccgagg gtaacgagca agaccaacca cggccgcatt ccctagagac aacaacgtga
     3721 caaccgcggt tgcaggaatc aggcgccgcc ggtgttcacc cggctcgtcg agcgctgggc
     3781 cccgacgcca atcgtcatcg gtggtccaac tacttcgaag aagccatgtc ctgtcttgcg
     3841 gagcttggac tcgtgcggca ggagcgcgtc gacgacaccc tgcgcattga gcgacacgcc
     3901 gaggaacagc gtctgggtct tgagcgcgtt gggctcgagc gtcggcggcg gcttgtagtt
     3961 gcgcgagtag aacccgacgt cgacgtcgac gaagcgggac cacttcgaca ggtctcgaat
     4021 cgggcggatg gctccgaggt ggagcgcgat caagtacttc tccgcggagt agtcctccgc
     4081 gatgttccag cgcgagcagc tcggctgacc gggcacgcgc ttcgcgcagg gcgagtcgga
     4141 gtcgaacagg ttgtggaggt actggtccga gaaccagtac tcgacgcggt agtcgaacag
     4201 ctcgtcgagg cgtggcgaca ggtccagcgc gagcgcggtc agcgcgccga gcgtgttgaa
     4261 cgtgaagtcg cccggcgaga actcgtaata gaagtagtcc ttcacctcga cggcaaagaa
     4321 cagtccttcg ctcaacgtcg cggacacgag cgcgctcttc gtgcggtccc aaccgccggc
     4381 gcgaagcgct gccgttcctc ctcgtgcgag gaccatcgtc gcccacgcgt gaccgagctt
     4441 gtcggcgcca ccggcgtacg tgtgcttgcc gaaccaacca tcacgaccga agtcgtgctc
     4501 gtccttctcg ggatggtttc tgtaccaagc gatgtacgcc cacgccgaga agcccgcgta
     4561 gagaccggct acggcgagac ctgattcgag ccgatggtcc gtgcgggtac tgtctgcaga
     4621 tctcgaagga ccaaacacga gcgctggtga ttggtcgatc cacggcgacc gagttgcgac
     4681 attccgatca tgagtaggcg tctcactggc tactggttca gccactgcga cgccacgcaa
     4741 ccacgtgaca atcaacagag cgagcgctgc gagacgcacg cggcgaccct cccactggcg
     4801 ttgcgcaaaa acaagtcagc acccctcagc tatctgatgt tcagctgtat cggtcacggg
     4861 ggatgtggca acatgcgtca accaacttga gttggtgggt gaatcgtaat agtttgctcg
     4921 catctccttg atcttggtcg cggtcctata agcagctcgt tctccgcgac ctctcttttc
     4981 tagccttact cctgtgaaac ctcgcttgac ctgtcatgcc gtgattggct cgcgcctcgc
     5041 atctagaggc cagcgatgac aacgcatgtg ctttgggctc tcgtgaagct cctagcgctc
     5101 gtggtgttca tcaaccgcta cgtcgccggc gtgctgattc gcattctgcg cggcaagaaa
     5161 tgggatgaga cccgcgatga cttcgagccg accgtcacgg tcgtgatccc gatgttcaac
     5221 gagggcgaag cgatcaagga gacgctgcag agcctcctcg acagcgacta tccctcgcac
     5281 aagctgcaga tcgtctgcgt tgacgactgc tcgaccgaca actccttcga gatcgcgcgc
     5341 gagatggcgc gtgcctcggg cggcaagctc aaggtcatcc gcaatcgcgt gaacctcggc
     5401 aagcgccgct cgatcaaccg cgccgtgcgc agcgccgagt ccgagatcat cgtctcggtc
     5461 gactcggacg tcgtcgtcga cgcgaacgca atccgcgagc tcactcgtcg cttcaccgac
     5521 gatcgcatcg ctgctgtcgg cggctgggtc gacgtgcgca acaagcacga caactggctg
     5581 acccgcatgc aggtcatcaa gtactggtac ggctaccact tcctgaagaa cctcgagtgg
     5641 ggcttccgcc gcgtgatgtg cctctcgggc tgcctcaccg cgtatcgccg cagcgtcctc
     5701 gtcgagctcg agccggtcct cgaagaccgc accatcctcg gcgtctcgat caagtacggc
     5761 gaggatcgct tcctgacccg ccagatcatc aaggccggct acctcaccac gatgacgcac
     5821 gcggctcgct gccggacgtt cgtgccgaac acgctggcgt cgtacttctc gcagcagctg
     5881 cgctggcgcc gctcgaacat cgtcgactac tcgggtgcgg tcagccacgt gtggcgcctg
     5941 aacccgttca tcgcgattca cttcttcgcg cagttcgcgc tgctcgtcgc gtatccaatc
     6001 gcggtcgttc gcgcgctgtc gtccggctgg ttcttcaacg gcctgcagat ccatctctac
     6061 gtgatcacgc tgatgggtct ctactactgg ttccgcgtcc gcaagctgcc gaaggaagag
     6121 cgcgttggcg tgctctcgtt cctcccgatc gcgctgacga tgccggtcac gtacgcactg
     6181 ctcacgccgc tcgctctgtt cacgctcgac tcgggaagct gggagacgcg tggacacgag
     6241 gcagagcccg acgccgctga cgagccggtc acagacatcc acacgggtca gcacattctc
     6301 gtgccggcac ttgccaccga gcccgtccgc agccgcgccg tcgaccgtca tacgggttcg
     6361 caggctcagc ttccagctgc ttagcgctga tccctcggcg gctatttaag tgacagcggc
     6421 ccgattcttg gagtaggaat cagctgcgat ggaagctccg cggaagacgc cgaagttctg
     6481 gatggcggtg caaccgtcga tcatccgtct ctacaaggtc gcgggacttg tcgcgctgac
     6541 tgcgatcctc gtcggacttc tcggcttcct cgtcgtcaat atcttctact tcttcgacaa
     6601 cagctgggtc cgccccgtcg tgctgtcgcc gaaccatccg aaggtcgtcg aagcgtcgaa
     6661 cctcgtggcc gatgcgaagc tgcgccagag ccagctcgac accgagcaga tggagatcac
     6721 cgcggagctg ggcgagatcg atcgcgcggt cgccgcggat gacaagttca tcaccgatgt
     6781 cggcacgcaa gccgacgcgc cgaagtcgac cgaccagctg atgctgcgtc gcgaagtcga
     6841 caaggcgaag ctcgacaaag agaaccagca aggtcgtcgc gcgccgctga aggctcgcct
     6901 cgagacgctc agcctgcgca tcaaggatca agagcaggtc gtgcatcgcc tcgaagcgtc
     6961 gccgtacctg cgcgccgtca acggcaaggt cgtgctcgcg ttcgttccgt acacgaacct
     7021 caagcacgtc tcgatcggca cgaagctgta cggctgctcg tggggactcg tcatgtgctc
     7081 gcgcgtcggc aaagtgaaag cgacgatcga cggcgaggtg caggacatcc atccgcacga
     7141 ccagagcgtg cagcgcggca tcatggtcga gatcgagctg tcgactccgt ctgcagaggg
     7201 caacacagtg ctcttcgcag gtggaaagcc gctttggctc ttctgacccg ttaaagcgcg
     7261 agaaacgctg acgccgcaga catgtagggc gtcaggaatc gcggtcgata acgcgcatct
     7321 cgacgagcgc ggcttgtaca attgtggcca gcgcttcgag gggaccgcag ctgcatgagc
     7381 gacgacaatc agggtgaaga aaagaagacg atcatcgggc aaggcccatc ggcgggacgt
     7441 tacgccgtta gcaagccagg tgatcgaacc gtcgtgattc cgaacgccgc tgctgcggca
     7501 ccgcgaaagt cgccaacgat gccaggcgtc aacgtgacgc ccgagccgca gaagcgtcca
     7561 ccgatggtcg tcatgccgcc gtcggctgca acggtcgtca tggcaccgca acaaccgacg
     7621 cctgccccgc ccgtcgtcgc aactggaacg ccgctgccgc agccggtcgc caatccaacg
     7681 atggcatcgt cgggccgcgc ccgtcgctcg tcggaagcgc cgtcgggcgt catgccgcgc
     7741 tctgcgtggg actcgccgag cggcatctct gggatctcgc cgggaagcac gcccggcaca
     7801 ccgccgagcg tcggtggtag cgatggcgcg acaggcgtcc acgcgaccgc gtcgaagccg
     7861 caccccggcg ttcgcatcaa tcagtacgag ctgatcaagc tgatcggcga aggcggcatg
     7921 ggccaggtct atgtcgctcg cgacctgcgc ctcggtcgtc gcgttgcgat caagttcctc
     7981 ctgtcggaga cgcatcccga gctcacgcag cgcttcctcg tcgaagcccg tgcaaccgct
     8041 cgttgccagc acgagaacat cgtcaccatc tacgaagtcg gcgagcacgt ctcggcgccg
     8101 tacatggtgc tcgagttctt gagcggcaag ccgctgaccg cgcagatcaa gaacagcggc
     8161 aagctgccgt acacgcgcgc cgtcgaggtg atgacgcaca cgctgaaggc gctcgaatgc
     8221 gcgcacggcc agggcatcgt tcaccgcgat ctcaagcccg acaacatctt catcaccgac
     8281 gccggcaaca tcaaggtcct cgacttcggc atcgccaagg tgctcgaagc gagcccgcag
     8341 aatgcggtgc agacgtcggc cgccggcatt cgcttaccga gcccactcga gctcgccacc
     8401 ggatcgaaca cgagcctcac gcggcacggc acgatcatgg gcacgctcaa gtacatgtcc
     8461 cccgagcagt ggggcatcgg catcgagatc gatcacctga gcgatatctg ggcgtgcggc
     8521 atcctcatgc accgcatgat caccggcaag cacccgctcg caccgctcga cggcaaccag
     8581 ctcgtcgtca ccgcgatgct cgagctgccg atgccgtcga tggccgagtc ggcgccgccg
     8641 gactgcccca aagagctgat ccagatcgtc gacaagtgtc tctacaagat caaagagcag
     8701 cgctggcagt cggtcagcga gttgctgcgc gcgctcgagc cgttcatgcc gggccgccgc
     8761 acgcagaccc tgcagatcga cgagagcccg tacgcgggtc tgtcgtcgtt ccaagagaat
     8821 gacgcgggca agttcttcgg ccgtaaccgc gaaatcgcgg cgatggtcac gcgcattcgc
     8881 gaccgtccgc tcatgggcgt cgtcggtagc tccggcgtcg gcaagtcgtc gttcgtgcgc
     8941 gccggtctcg tgcccgcatt gaagcgttcg ggcgagcagt gggaaacgct cgtcacgcgc
     9001 ccgggtcgtt cgccgatgga cgcgctcgcc gtgatggtcg cgccgatggt cggcacatcg
     9061 tcgaacctcg ccgacgagct caacgagcag accaagctcg ccgctcgcct gcgcaaggag
     9121 cccggtcacc tcggtcacct gctccgtgga cgcgcgcgca aagaatcgcg ccgcatcatg
     9181 ctcttcgtcg atcagttcga agagctctac acgaacgtcg aggatccggc ggagcgcgcg
     9241 gcgttcaccg cggccctcgc ggctgtcgct gacgatgcga cgagcccgct gcgcgtcgtg
     9301 ttgtcgattc gctcggactt cctcgatcgc tgcgcggagg accagacgtt cctcgccgac
     9361 ctcacgcagg gcctgttctt cttgaaccag cccaaccgcg acagcttgcg cgaagcgatc
     9421 acgcagcccg ccgagctcgc cgggtactcg ttcgaggtgc ccgccaccgt cgaggacatg
     9481 ctcgaccacc tcgagacgac gccgggcgca ctgccgctcc tccagttcgc cgcaagcaag
     9541 ctgtgggagg gccgcgacgt cgcgcgcaag atgctcacgc acaacgcgta cgctgcaatg
     9601 ggcggcgtcg ccggtgcgct cgcgagccac gccgatcgcg tcgtccagga gctcggccag
     9661 agcaatgcac cgctcgtccg cgcgatcttg ctccgcctcg tctcgccgga gcgcacgcgc
     9721 tcgatcgttc cgctcgacga cctgcgcgag ctgtcgcgtg aagtcggtgc ggtgcagcgc
     9781 cttgtcgatc agatggtcga cgcgcgcctc ctcgtcgtgc agacgctcga aggcggcaaa
     9841 ggctcgaccg tcgagatcgt gcacgagtcg ctgatcaaca actggccgac gctgcgccgc
     9901 tggctcgatg aaacgcagga agacgctgcg ctcatcgatc agctgcgcac ggcggcgcgc
     9961 caatggaacg cgaagggtcg ctcgcccgat ctcttgtggc gcggtgacac ggccgacgag
    10021 gcgaagaagt tccggcgccg tcacaagggc ccactctctg atatcgagca gtcgttcctc
    10081 gacgagatca tcgcgtacga gggcgccgtc gctcgccgca agcgcgtggc cgtcatcggt
    10141 gcgttcgtcg gccttggcat cctcgtgatc gccgcgatgg tcgcgctcgt gatcatccag
    10201 aagtcgcgta ccgaggcgaa ggaccaagcg gtcatcgccg agcagaaccg caaagaggcc
    10261 gaagagaacc tcgcggcgat gcgcgcgaaa gaagccgagc gccagaaggc cgaagccgag
    10321 aaggcgaagg ccgagcagaa ggtcgtggtc tccgaggtga agctcgacgc tgcgcaggtc
    10381 gacctcgcga agaagaacca ggagctcgaa gccgcggtcg tggtggctcg agagaacgaa
    10441 gccgaagcca agagggctga agcgcgtgca aaggccgccc aaaaggaagc cgagcaagcg
    10501 cagggggaag ccgtcgcggc caaggatcag gttgcgattc agaagatgca ggtggagctc
    10561 ttgctcaagc aggaacaaga gcgcgtgaag cgtttgcagt cgcagatcgg tagtccgatc
    10621 gtcgacgatc tcaagtagct actaactttg gagacactat gaccaaggcc atctgggggt
    10681 tggcactggg gctcggcatc gtgctgagcc tccgcaccgc gcatgcgcaa gcgccgcaac
    10741 ccgaaacgcc gcccaagccc gagaagacgc cgaagtccga cgagaaggcg gacacgacgg
    10801 aaacgctcca gaagggcggc gacgtccggc cctgggcaca aggtgttccc cccgccgagc
    10861 agaagacggc gctgctcgcg ttccgcgaag gcaacagcca gctcaacgat ggtctgttcg
    10921 cgaaagcatc cgagacgtac cggggcgcgc ttacgcactg gaagcacccg gccatctatt
    10981 acaacttgtc gctcgcgctc atgaacctcg accaacccgt cgaggcgtac gagagcatgc
    11041 aagcagctac gacgttcggc gaaggcccga tcgacaagga caagttcgag cacgcgaagg
    11101 aatatctgct gctgctcggc aagcagatcg cgacgatcac ggtcgcgtgt gacaagccgg
    11161 gcgcgaaggt ctcgctcgac ggcaaggaag tcttcatcgc gccgggcaag cacacgcagc
    11221 gcgtgcgcat cggacgccac acgctcgtcg ccgagaagac gggctatcag gcccgcgtca
    11281 acgctccgaa catcggcccc ggcgaaacgt tctccgtcga gctcaagctg ttcactgctg
    11341 acgagctgac gcgctatcgc cgccgctggg agacgacgtg gatgccgtac gcagtgctcg
    11401 gtgcgggcat tggcgtcggt ctcatcggcg tcggtctcga gctcggcgcg gactccagtt
    11461 acaacgatta cgacaagaag gttgccgcct gcagcatggg caacacgggc tgcgagaaca
    11521 ctcaggcgct gaaggacctg cgctctcgcg gtgacacgat gaagacgctc ggctacgtga
    11581 gctacggcgt tgccggcgcc acgatcgcgg gcggcatcgt cctcgcgatc ttgaaccgtc
    11641 cgaagtcgta cttgctgcgg cccgaagagg ttcaagacca gctcgatcag aaagtcacgt
    11701 tcgctccgat cgtcacgccc acgatggcag gcgccagcgt gcaaggacat ttctaatgaa
    11761 gtatctcctc ctcgccttat tcatcacggc gtgcgcatcg acgcccgact cgcagatctg
    11821 cgagcgctcc gggatcgtct gtcccgaggg aacgaagtgc gctgccgtgc aggcggtgtg
    11881 catcaccaac gattgcggcg atggcatcac cgcgggcggc gagacctgcg acgacggcaa
    11941 catcatcgat ggcgacggct gctcggcgga ctgtcgctcg aaagaatcgt gcggcgacgg
    12001 catcctcagc gccgctggcg gcgagctctg cgacgacggc aacgacgacg actgcgacgg
    12061 ctgctcgcac gactgcaagt cgatcgagct ctgcggcaac gacatcaagg attgcggcga
    12121 agcctgcgac gacggcaaca ccgtcggtgg cgacggctgc gcgtcgaact gcaagtcgac
    12181 cgagagctgt ggcaacggcg tggtcgatac ccaggtcggc gaggtctgcg atccacccgg
    12241 tgcgaactgc ggcgtcgact gtcggtcgct tcttgcgtgc ggcaacggca tcgtcgatgc
    12301 cggcgagcag tgcgaccacg gccaagcgaa caacggtgac gacaaggact gcacccacga
    12361 ttgcaagatc aacgtgtgtg gcgacggctt gatcaacttg gtcggccccg accacaaaga
    12421 gcaatgtgac ggcggcacga ccggctctcc caaggagacg aacctctgca acctcgactg
    12481 cacgaacgcg gtctgcggcg ataacaagat caaccagacc gcgggcgagc agtgcgattc
    12541 gacgagcggc aacgactcgc cgagctgcaa tggtcagaac ggcggcgcgg tgaagtgcaa
    12601 gctgtcctcc tgcggcgaca actacatcaa cccggcggcc ggcgagacgt gtgacatcac
    12661 gggcggtgcg gactcgccga gttgcaatgg ttcgagcgca ggccagaagg cctgcaaggc
    12721 cgccgtctgc ggtgacaact tcctcaactc gcaagcgggc gagac
//
LOCUS       DATLBZ010000460        26872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9517767, whole genome shotgun sequence.
ACCESSION   DATLBZ010000460 DATLBZ010000000
VERSION     DATLBZ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 26872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26872
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9517767"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VMZ53_23190"
     CDS             <1..787
                     /locus_tag="VMZ53_23190"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HUS31439.1"
                     /translation="QIFAADWGLIFYFAVLTLANYGGTIAGWASYNKWALLGGLRSSS
                     QRRSYEVAMGLSLLGAVLVTGTLEPGWIAAHGATNTLSASNPLNWLWLWQPVGLVLFF
                     TAAIAETKRAPFDIPEGEPEIIGYFVEYSGMRWGIFFLAEFIEVVFIAAVVATVFFGG
                     YQVPFLDADGFRFGGYMSDVNGHGVSAGGWVFHLPHIVVTSLQFAAFGLKVVLLCWFQ
                     LMVRWTLPRFRADQLMNLGWKLLLPLSLANIMVTALIKLIFTS"
     gene            797..1507
                     /locus_tag="VMZ53_23195"
     CDS             797..1507
                     /locus_tag="VMZ53_23195"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HUS31440.1"
                     /translation="MSVSTHPEQDGHGEESDGPKVLAEAKSKHGKVMRTIQVVRPEPE
                     DSSFLAASAKGFGITLKHFAKNLFLPLKKLSNRSRAGAPLDEGMRNDIETVQYPEEKV
                     TYPERYRGLHRLMLREDGAVRCVACMCCPTVCPAHCITIVPAETDNKGIEKRPAIFEI
                     DELRCVVCGLCVEACPCDAIRMDTGDHAKPVEDRADAVESLESMLARGIKSSAKQGGE
                     GPNWREGGHEASKHEVNR"
     gene            1679..1853
                     /gene="ssrS"
                     /locus_tag="VMZ53_23200"
     ncRNA           1679..1853
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VMZ53_23200"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1982..3502
                     /locus_tag="VMZ53_23205"
     CDS             1982..3502
                     /locus_tag="VMZ53_23205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31441.1"
                     /translation="MNRRDTGVTAVTSVTEVAAGKGPARGSMLLGKYRVESTLGFGGM
                     GLVIKAHNIDLGQDVAIKILREDVQIDEENIARFIREAKNAVRLKSEHVARIRDVGMF
                     DDGRPYMVMELLEGLDLGKLLLDTGRIEPVRAVDLVLQACDAIGEAHSLGIVHRDIKP
                     TNLFVAKRPDGTDIIKVLDFGISKAAQHGTEMVLTQTQSMLGTPAYMSPEQMRSARTV
                     DPRSDVWSIGCVLYEAVEGHAPFEASNFAELCVMVATEDPAPLVHAPELGGVLMRCLA
                     KTLEERYQDIAELAADLAPLSSDPTSAANQVARIQRLLQNRADGTPPPWRKSDHRISE
                     PSVPRMMVSSTPLPGAPSSVVDAAPPLDTVHDVRTGGGVLKTLAVLVLLFAVGIGAGL
                     YVTRDRSSTDSQAIAPQDAELQVPATAPIDAAAAPEPPDAREITVEKGSNAAGSGSAT
                     PHVQPPPKQPPPKKPPPKKPPPKKPPPPPQGSGAGSATIVKPPPKCDPFDNKNGCS"
     gene            complement(3503..4591)
                     /locus_tag="VMZ53_23210"
     CDS             complement(3503..4591)
                     /locus_tag="VMZ53_23210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HUS31442.1"
                     /translation="MAAHHDSGFTFELVWKIGLVLLLAIPIVGVLMPRYARAWFRESW
                     PKLLQLIGMAAVMGTLIHYAQLLPAVSILSATLVRIIASLIAAGAMLWLIFRDPTFVA
                     WFKYISVDQWRAIDAETDRPPEAAGRFDWRILVVLVVVALSLTLQEYIGDRGQFERWF
                     PSHGPDPYYELKGFAWWSGWRVLGYVIMPWTAVALMPGERIRDYHVSPKGFFSHLWLY
                     VMMFALILPAVIIASTTQAFRHTYPFYRMANRSHFDLWSWEALYAAQFLSLEIFFRGF
                     ILQGLRRALGSNAIFVMIVPYCMIHYGKPLPETLGAIGAGLILGTVAMRTKSIWGGVL
                     IHVGVAMTMDVFALRGCPPIGSNRWCGS"
     gene            complement(4591..6048)
                     /gene="gltX"
                     /locus_tag="VMZ53_23215"
     CDS             complement(4591..6048)
                     /gene="gltX"
                     /locus_tag="VMZ53_23215"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HUS31443.1"
                     /translation="MKPVRVRIAPSPTGDPHVGTAYIALFNYVFAKKHGGKFVLRIED
                     TDQTRARSDSEQMIFDALHWVGLSWDEGPDVGGPYAPYRQSERSAIYKEHADMLLAKG
                     EAYRCFCTEARLAKVRVEQQAAKAGTLGYDRHCRDLDPAEGAKRAAAGEPNVIRLKVP
                     LSGPIEFEDKLRGKVTREAKEIDDQVLLKSDGMPTYHLANVVDDHLMEISHVIRAEEW
                     ISSTQKHVLLYKAFGWDAPEFIHMPLLRNADKTKISKRKNPVSINYYRDIGILPHALL
                     NFLGLMGWSFGGDREKFTLEEMTDVFSWDRMSLGGPVFDLNKLTWLNEQYIHEQSYDQ
                     LADALINWRVNKDYLLKVLPLVRERIKKLDEIIPATEYFFSGDLDYSAVLSQMTIPEV
                     AATDLKKALLDLVERFEAKDGWSKEMLEEVARGWVEANGWNSGKAFMLLRIAITGRTA
                     SPPLFDVMAVLGKEIARRRLRRAADVVGAGGKLKS"
     gene            6087..7232
                     /locus_tag="VMZ53_23220"
     CDS             6087..7232
                     /locus_tag="VMZ53_23220"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HUS31444.1"
                     /translation="MLSLLVATSLTGYVSDVQSHWTADGTRIITEATLHTGAGDVTVS
                     QFGGTAVDPQTGERLSMREMPGPEPLLDGMRVAISPSFAMDNSWRMHVVVDSMKVLDY
                     PAGFVRTGPTAAGHYLYWESGCVFITVDSDGTKEIAGDNEFPLISESIATWNDNTASC
                     SYIQLREEGKKAVETGRDRVNIIKFRDQSWCRPATKDDPARCHPDSAAGITTATYVDD
                     SGSSRDGAIVDSDVELNGVNFAISVNGQTLGTEPCTSELKNTLTHELGHLLGLEHTCL
                     AAGDPPRIDNNGAAVPLCTQAMSNMTITAATMFNYQACGEVTKSTLEPDDINAICTIY
                     PIAQDPGTCEHVGDGTGCCDSSGNPGGSLLLALGVLTGFWRRTRRAR"
     gene            7242..7805
                     /locus_tag="VMZ53_23225"
     CDS             7242..7805
                     /locus_tag="VMZ53_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31445.1"
                     /translation="MTGPVCPSCGVAVMPGYARCPKCRKPLPRRKQTVMGGTAVDSTS
                     RGPLLAILAAAIVGAGIIAYIGLRSSQSSSAAPRAATPTQQTDTTANDVVESNVPDQP
                     ETRTNTPAAPDPSAVADRLEKALKRERLWSTVSVEGGRIDVRSGSCSDPKMAGILDGS
                     SQSLKSAGLTRLRCLEQSGQVVSDRDL"
     gene            7795..8154
                     /locus_tag="VMZ53_23230"
     CDS             7795..8154
                     /locus_tag="VMZ53_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HUS31446.1"
                     /translation="MTSDSEPPPSKPHRLILPLGMRVLVQVIRDEERTDAGLYLPAGA
                     KEAQDDALYGKVIEVARDRPTTNEATENVSGVPHGAHVLFRKDAGVRVPWDDRLRVID
                     VKDVLATVEEVRDSEAH"
     gene            8219..10741
                     /locus_tag="VMZ53_23235"
     CDS             8219..10741
                     /locus_tag="VMZ53_23235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HUS31447.1"
                     /translation="MDVRCEKCQTEYELDESRLKPGGVTVKCTNCGHMFKIRKRANTN
                     AGVASAPAAKAQPTPAPPPPAPAAAQPAPVSDRSKRGDAEAPVNIADMPSGPQSASAE
                     RQWLVRMENGEQKTCRELATLQQWIVAGIVSRESLISRSGKTWKRLGDIGELGQYFVI
                     ADEARANRAAKPTGPSAKGAAGAGTMLGVGAMRPSTNPDDDLEQRSTGNFRARPPTPP
                     PPPPASMSGSGSKANPMAQTELAPSGPTKRPPTNPPPPPVSKPKTMEPPPGRATAGWA
                     SNDVKASESMAAMPQGPRGGKLSASPDEPAFAGKVRVTPSDRSTFDGRVVAEDDDEFY
                     PASRGSRAGMWIALISLLVIGAAAGAVYMFVFKAKQSPTAETTKQDGGIATAGDASSV
                     VVAPIVDAQEVASSPLDTARAELVGGVHTRMKSTLDGLAGKDDPASLAMRARLMTAMA
                     QGMNDRAGFVDKAEADKLRKEAKQIVIDAAPLAQRAVAAQADSVDANLAMADIMRLQA
                     KPAPSVKRYITAAKGKGDKSAAASVALSEAQLAIRDGKLADATTALSSAEAPADDMRI
                     KLALALIAYAQAKPADAKAHVDQILATQPDHDAALAMYKKLETTVSSSDPLPPEDGSG
                     AKPNNAGSGSTTTNNGTGGNGGNGGNGGNGGNGGGNGGGGGGGGGGDSYDALLAKANK
                     LAESNCSAAMPIFQKALDQKPNGVEALTGTGYCHLDRKEFSSAFSKFRAALLVSPRYE
                     PALGGVAETYQRQGNKPAAIEAWNKYLEVFPGSAKAKKQLEILGADTTGGGTTKPPEG
                     GGSASGGDTKPAGGGDTKPAGGSETPPAPAPTPAPTPAPATP"
     gene            11066..12982
                     /gene="sppA"
                     /locus_tag="VMZ53_23240"
     CDS             11066..12982
                     /gene="sppA"
                     /locus_tag="VMZ53_23240"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HUS31448.1"
                     /translation="MRAALVAIICLAACKSNPSPLDHAFGSGSYAGPTDDPWAATLGT
                     KNEDGTSSRSGDGGFDLQGILEKVKEAIDTPGPYESPKQTKDFAVDKPHWGVMGLDGE
                     IVEREAFSITGGGGTELRHLVVRLRALAKNEQLQGLFLRIEPLSISLPDLMELRAAMH
                     DFRAAGKKLYCHTESAMNATYLVIAACDRIALAPLGDIALTGPSAMPIHVKPLLDKLS
                     ITADFIHVGAYKGAAEPLTRDAPSKEMEETLGAILDRRYQTIVDVVAAERKLEPATVK
                     ALIDEALFPADVALGKKLVDEVTPWEQFRDSVKAPWAKMEIEPDDKDQLQAMMKLARF
                     VGAMPAERPFGDHVAVVYALGDIKDGDGDGVIGARQEIASHTLVSALRAIAADDSVKA
                     VVLRIDSGGGSAQASELIWTAVEQLKAKKPVIVSMSDVAASGGYYIAAGATKIFAQED
                     TLTGSIGVVGGHIAPAAALAKLGVNTFPMGRGKHATLMSSFQPWTDDEKKLMQTHMEA
                     VYKVFVSRVAAGRKKTAEQIQPIAQGRVWVGTKAKELGLVDEIGGLDAALAEARKLGK
                     VDEAIDVEVYPPAPTLRDVLAGWGQVHTPFGLSMQLQLLDPEIAKVAGHLLALVDAFR
                     TTPIQTIAILPAIQ"
     gene            12979..14016
                     /locus_tag="VMZ53_23245"
     CDS             12979..14016
                     /locus_tag="VMZ53_23245"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HUS31449.1"
                     /translation="MKCLVLVVALAACSSQQTTAPQQKSPAPTAAPVAPANAGRVETR
                     HFQSAALGVEKDYIIYLPAGYDASTKRYPVFYYLHGLGGNETNWTNTGELAQSADKLG
                     LEAIVVMPDGDDGFYADSAAPIDYDTCMKDGTGLFLPGMQNRQHTCVKQRKYETYIIK
                     DLVGEIDAKYRTVASREGRAIAGLSMGGFGALQLGMRHPDLFAAAASHSGVDALLYAG
                     PYPYEKGKVKLITQEEISRYGKGNPDVEQIGLWIQMIFGKDIAVWKSYDPATLAEKVE
                     PGKPALYLDCGTEDGFGLQNGAQYLHDILSARNIEHAFFLGPGPHDMRFWRPRLPESL
                     KFLRDHTVKPT"
     gene            14026..17049
                     /locus_tag="VMZ53_23250"
     CDS             14026..17049
                     /locus_tag="VMZ53_23250"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS31450.1"
                     /translation="MKLVGRDRELEQLRDVCRQARDGRGRVVVVSGDAGLGKSALVEE
                     IANELAPAFTITWGRAWELADAPAYFPLWPCLKSIGVAFDGRGDETGAFELWEHVLEA
                     LVRAAATKPVMWIVEDLHVADTQSLDLLNFLAQAVRPLPVMLLATTRAQAADAAHKRI
                     VRLARMGIELRLAPLARENVDVLAKHWLDRDLSADELAQLSERTGGNPLFVIECARAG
                     KGNGKHLLGAVPPTVAVVVGERVEQLPAATRTVLADAAVLGNELTAATLGAMTDKLPA
                     RVIDDLAPALRAGLIEELAPGRFRFHHALVRDAIEDQMAPAQRRAAHQRAEAALSSGI
                     DSPRAIVESARHALLGVEAGREAHVTDIVERALSVLEEQGTFDRASQLYRQLRDLRAN
                     GVLPGGTPSDLIRGAAIARRAGQFAYSRELSLEAATRARATGDTEQLAYAALAVGAEI
                     HPGVVDPVVVQHLEEALAKAPAGPLHCRLTARLAAAMQPHADPRVPIDMAREAIREAR
                     KLGDAALLEDVLYVATSAMIEYVDAEQVRDLNREHLALARTHADAERQIRAYTRLLFN
                     EGELGDFAMFDKLLDEMMLVATAAGHPRVTWRALLMSSMRAIATGQFAESERFISEVE
                     RAAQLIDDLGLSMSLSAHRHMRAVDLHRETTVQQAITSIEASIGANLPGRAFIVPMIK
                     GIAMSRFGDVGAAGRAWAALPLDDLFSRGGGTVGWLSVIGEIAACVGTDEQRKNILER
                     IQPFASRHVYFGHTPMIYGGPVRRVIGLLELSLGQKAAGENSLRRALDVCRTNGFAPW
                     VARISFELGEMAEAARLATNLGIRGLAKRAAVPEAESVAPPTKPALGVSREGELWRIS
                     YGVRVARVSDARGMELIAKLIERPGEDMHVLVLAGTGGEAMTDSDAGDALDERAAKSY
                     RERIAAIDGEMPVAKGERANQLARERAFLQGELARAFGLGYAPRRVGSMSERARVNVQ
                     RRIKDALTRIGHHDPVIADYLRSAIRTGTYCTFRP"
     gene            17115..17882
                     /locus_tag="VMZ53_23255"
     CDS             17115..17882
                     /locus_tag="VMZ53_23255"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HUS31451.1"
                     /translation="MESIIPALFPLTYFALLLAERLLGGRRFPKVRWWIPKGILFFFI
                     GGLINSTLPVALARVFAGHTLLDLSSLGLAGTVVAVIASTFVSYWLHRTFHRFSGLWR
                     WTHQMHHSAERVDMAGFGYSHPFELLIAVGMTPLVASIIGVAPEAAMLAGYLTFVLGL
                     VEHFNVKTPQWLGYIVQRPEMHCVHHERGVHAYNYGLPLWDMLFGTLRNPKAWSGQAG
                     FWDGASKQTFAMLAGRDVAVPPHVAAVANAVPERIAA"
     gene            complement(17879..21337)
                     /locus_tag="VMZ53_23260"
     CDS             complement(17879..21337)
                     /locus_tag="VMZ53_23260"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS31452.1"
                     /translation="MATLVYTDVDGVDRSFSLGNEPVTVGRAPDCTIRSEDPRVSRMH
                     ARFFVDQGTLWVEDLGSSNGIYVGPQKTQRAPVPVGEIILIGSLMIRLLPASGTLPPP
                     MGLHGTLAQWLEMERKARVAVEEERNAFAQRVGELHDEIRIMREAQNLLQEEERTLRT
                     ELDDLRRKSVADLEQVRVELAKAREEKIVATTSAGLTAVEKLAEQDAIISRLENELIT
                     ARAQVHATAAEPKSKILEDQLVTSTARAEKAEKDLASAQIRAQGAERNLQHANMQAAK
                     AETKAAELQSKIEELSAEKAKLATEIAAEKSKAGSELATANEKIKQLESRLGQGAAPV
                     QAAEARATALAAELAEANAARDAAKARAADLEKDLIEAKDATLAADAQLQQARAARGE
                     LEAKLQADAGAQAEKLAAANAKVKELEARLEQLKGAEAEMAAAKKEREEARSQTAGAD
                     KKIAESLARAEEAENRARAADTMAKAMAKDVAEALRRAVDSDSKSRAVTRELDGAHKK
                     AAEGEKKAADAAALIKAAEDRAAAAEAARIANEKELGDKLEQLQKELVDKLGSTEKEL
                     VAKAEKVKQELEAKLDQTARELAAERSTAMSLVDRKTQLERELNEARTTLAGLQHRLE
                     LAEGKVAEYEVQVEQLEDRIQDLEAGAAVEQTAASSSIEEARERIKKLEEQLQESKAA
                     GKTASKTAAGLERRLDALETELKTVKEARAAAEAALTEARARVGDLERSVEEYTTTVH
                     TDRSLIEAAESRAKDAEAKLEPLAAKAEAADLAIGRANALQRQLDESLQKLSWMERDL
                     ENAKAKNPAAEAEVQRRIDEAEARARDAEGRALDADARVEAQTAMARDADARAREAEV
                     QMAAAQARAQEVEGRARDIETRAKDIEARAKEAAERIVEAERRARDAETKLADAIRRA
                     QDAEDKLEDLQGTQERSDVGEVAALQARVAELQAEVDRADNVRQFAANTEREIAGLER
                     ELREMKAKVTQITLERDRLESQLRDLRDDSETTNRRVPAVGGGRADPEVTAQADLSRY
                     TALVARAAELEQKVMKMEKDGERMRRELSESEQKLAAQRRANEDEPTNTGASIPMEFA
                     EYLSVLEESIDSLRANMRAASDETAMMDQSDSVVAVGQAVSSAAEHVERARDALRSLM
                     AMVPQN"
     gene            complement(21359..22495)
                     /locus_tag="VMZ53_23265"
     CDS             complement(21359..22495)
                     /locus_tag="VMZ53_23265"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HUS31453.1"
                     /translation="MKPLGPGSTIGVLGGGQLGRMMAIEARRMGYRVVVLDPSARCPT
                     AQVADGVVVGALDDLESAKHLARQVDVITLDTEHVPADILAELEKLVPVRPGASVLGT
                     INDRQIQKQFLDKLGLPQAQWAPATTEAELAAALKQLGRPAILKVRSRGYDGKGQVRI
                     EESSDASADLAKLRGAPVVAEELVRFKREISVILARGIRGEVRTYPIAENVHRRHILH
                     TTRAPAPMTPATQKRAEEIGVTVAKQLGHVGVIAVEMFELAGGQLLVNEIAPRTHNSG
                     HYTWGACVTSQFEQHVRAVCGQPLGDPTAMSGSVMVNLIGDLWHSGPPKWDTVLARPE
                     ARLHLYGKDLAAPGRKMGHVLLLDDDTDRALEAADQLVSTLSRA"
     gene            complement(22492..23052)
                     /gene="purE"
                     /locus_tag="VMZ53_23270"
     CDS             complement(22492..23052)
                     /gene="purE"
                     /locus_tag="VMZ53_23270"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HUS31454.1"
                     /translation="MSKPKRAKATATATATATATATKPKVGIIMGSQSDWPTMQKAAE
                     MLERLGVPYEAEIVSAHRTPDKLFNYANTAADRGLLVIIAGAGGAAHLPGMTAAKTRL
                     PVLGVPVQSKALSGLDSLLSIVQMPKGVPVGTLAIGEAGAANAGLLAASIVALQDPKV
                     ALALDVFRKQQTDDVLAQALDDGDTK"
     gene            complement(23049..24101)
                     /locus_tag="VMZ53_23275"
     CDS             complement(23049..24101)
                     /locus_tag="VMZ53_23275"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HUS31455.1"
                     /translation="MKRGTDIAEAVRVLRAGGLVAFPTETVYGLGADASRDEAAAKIF
                     AVKGRPAVHPLIVHLPSDSRIEGWALEIPENAMRLARKAWPGPLTLILKRGHRVAKAT
                     TGGGDTVGLRVPSHPMAQQLLHAFGGGIAAPSANRFGAVSPTTADHVAADLGDDVDYI
                     LDGGACSVGVESTIVDFSRGRPVLLRPGGMTRDAIERMVGEALLEPDEIEEAAPAAPG
                     TLASHYAPHAEVIAVEPHEVPATVMRIAAELSAKPEPTTEASPSGRVKRATMVRERIA
                     VLAPAAEFPAWGPIAARAYPLPDDLPGMARELYAALRDLDGMGVEVVIATLPPAVGLG
                     EAVGDRLRRAAGPRSP"
     gene            24137..24643
                     /locus_tag="VMZ53_23280"
     CDS             24137..24643
                     /locus_tag="VMZ53_23280"
                     /inference="COORDINATES: protein motif:HMM:NF026926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm10 family immunity protein"
                     /protein_id="HUS31456.1"
                     /translation="MMMLRALAVGVDDSEELQCFTLGFAEEADGSGPALMFQRAYAFD
                     EQDIELGFDTYCLVTDSQSTTYGGIEKWQLAEGWLRLSLSFKAAEDLGVDRDLLIQFA
                     PVHMELVRSALARILAPVEAPVAADATQPKAAKAKRAKAKRAKAKRAKAKTKTKSEAR
                     AKTKSARR"
     gene            25797..>26872
                     /locus_tag="VMZ53_23285"
     CDS             25797..>26872
                     /locus_tag="VMZ53_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31457.1"
                     /translation="MLGNERVIDTSHKPSTAQIDRALRDLAQQHAALEADEAHWLRLA
                     DQQKIWPKLGYVHALEYLEDVFGYAPRTAKDRIRVARELGDLPQLEDELRCGELSYSV
                     VRELTRVATPETVERWIEQARGRRLRDVEQMVSGRKKGDDPDDKPDANLVKHTVVLEL
                     DGASMAIWREMQAVLEAELETSLDDRALVLALGDRILGAASTAVRDRAPSNDNEIDGP
                     DAPDAPAAAGAADAAYAPDAVADTSERLRATKTIPAATKRRVLERDQGRCQVPGCRSR
                     KHLQFHHIEFQSHGGGHEDSNLLLLCGGHHRLLHDGLLTIAGRAPNALVFTRNGQRLR
                     SADDGAQPQLSGRDTHATSKHAPVP"
BASE COUNT         4581 a         9144 c         8697 g         4450 t
ORIGIN      
        1 gcagatcttc gcggcggact ggggcctcat cttctacttc gcggtgctca cgctcgcgaa
       61 ctacggcggc acgatcgcgg gctgggcgtc ctacaacaag tgggcgctgc tcggcggtct
      121 gcgcagctcg tcgcagagga ggtcgtacga agtcgcgatg ggcctctcgc tgctgggcgc
      181 ggtcctcgtc acgggcacgc tcgagccggg ctggatcgca gcgcacggcg cgacgaatac
      241 gctgtcggcg tcgaatcccc tgaactggct ctggctgtgg cagcccgttg gcctcgtgct
      301 gttcttcacc gcggcgatcg ccgagacgaa gcgcgcaccg ttcgatatcc ccgaaggcga
      361 gcccgagatc atcggctact tcgtcgagta ctccggcatg cgctggggca tcttcttcct
      421 cgccgagttc atcgaggtcg tgttcatcgc ggccgtcgtg gcgaccgtgt tcttcggcgg
      481 ctaccaagtg ccgttcctcg atgccgatgg tttccgcttc ggcggctata tgtcggacgt
      541 caacggccac ggcgtgagcg ctggcggttg ggtgttccac ctgccgcaca tcgtcgtgac
      601 gagcctgcag ttcgcagcgt ttggcctcaa ggtcgtgctg ctctgctggt tccagctgat
      661 ggttcgctgg acgctgccgc gcttccgcgc cgatcagctc atgaacctcg gctggaagct
      721 gctgcttccg ctgtcgctcg cgaacatcat ggtcacggct ctcatcaagc tgatcttcac
      781 gtcctagagg tccccgatgt cagtttcaac gcatccagag caggacggtc acggcgaaga
      841 gagcgatggg cccaaggtcc tcgccgaagc gaagagcaag cacggcaagg tgatgcgcac
      901 gattcaagtc gtgcgtcccg agccggagga ctcgagcttt ctcgccgcga gcgcgaaggg
      961 ctttggcatc acgctcaagc acttcgcgaa gaatttgttc ctgccgctca agaagctcag
     1021 caaccgctcg cgtgccggcg cgccgctcga tgagggcatg cgcaacgaca tcgagacggt
     1081 gcagtacccc gaagagaagg tgacgtatcc ggagcgctac cgcggcctgc accgcttgat
     1141 gcttcgagag gacggcgcag tgcgttgcgt cgcgtgcatg tgctgcccca ctgtgtgccc
     1201 ggcgcactgc atcacgatcg tgcccgccga gaccgataac aaaggcatcg agaagcgccc
     1261 ggcgatcttc gagatcgacg agctgcgctg cgtcgtctgc ggcctgtgcg tcgaggcatg
     1321 cccctgcgat gcgatccgca tggacacggg cgatcacgcc aagccggtcg aagatcgcgc
     1381 agacgcggtc gagtcactcg agagcatgct cgcgcgcggg atcaagtcga gcgcgaagca
     1441 gggtggcgaa ggaccgaact ggcgcgaagg cggccacgaa gcgtcgaagc acgaagtgaa
     1501 tcgctaaaaa cgcagacaca gatctggaat cgcgtgcgca aaaagatcag cgtgcgcgtc
     1561 ggtcacagcg cagtcactgc gatcgcgacg agcttctgcg tggttgcaaa gaagtgatcg
     1621 cttcgatcat ttttcccgtt gacggattcg atcacgaacc gtagctttgt cttcagagca
     1681 ccggcggttc gatgcgagaa ccggggcgaa actgtctcct acatttctat ccaagggatc
     1741 tgcctctgat tcgggagtgc acgcccgctc gtcgggaagc ccgaagaatt actgtggagc
     1801 ccacctacgg caatcgtagg ggatggagcg tcctacggtc gaacggtcgt gctcgttttg
     1861 gggtgcagcg gaaaaaaggc cgctgcaccc cgtctgtttt ttcggggtcg tagcggcgct
     1921 tacccctgcc atcaccatgt aatggcccgc ctggcagtgt ttcgggctac gatcatttca
     1981 cgtgaaccgg agagacactg gggtcacggc ggtcacgtcg gtcacggaag tagcggccgg
     2041 caagggcccc gcccgtggca gcatgctgct cggcaagtac cgcgtcgaat ccacgctggg
     2101 atttggcggc atgggcctgg tgatcaaggc ccacaacatc gatctgggcc aggacgtcgc
     2161 gatcaagatc ctccgcgagg acgtccagat cgatgaagag aacatcgctc gatttattcg
     2221 tgaggcgaaa aacgcagtcc gcctgaagag tgagcacgtc gcccgtattc gcgacgtcgg
     2281 tatgttcgat gacggcaggc cgtacatggt catggagctg ctcgagggcc tcgacctcgg
     2341 caagctgctg ttggacacgg gcaggatcga gccggttcgc gccgtcgatc tcgtgctcca
     2401 ggcctgcgac gcgatcggcg aagcgcactc gctcggcatc gtgcaccgcg acatcaagcc
     2461 gacgaacctg ttcgtcgcga agcgtcccga tgggaccgat atcatcaagg tcctcgactt
     2521 cggcatcagc aaggccgcgc agcacggcac cgagatggtg ctgacgcaga cgcaatcgat
     2581 gctcggcacg ccggcctaca tgtctccgga gcagatgcgc tccgcgcgta ccgtcgatcc
     2641 gcgcagcgac gtgtggtcga tcggctgcgt gctgtacgag gcggtcgagg gtcacgctcc
     2701 gttcgaagcg agcaacttcg ccgagctctg cgtgatggtc gcgaccgaag accctgcccc
     2761 gctcgtccac gctccggagc tcggtggcgt gctcatgcgc tgtctcgcga agacgctcga
     2821 ggagcggtac caagacattg cggagctcgc ggccgatctc gcgccgctgt catccgatcc
     2881 gacgagcgcc gcgaatcagg tcgcgcgcat ccagcggctc ttgcagaacc gcgccgacgg
     2941 aacgccaccg ccatggcgga aatcggatca ccgcatcagc gagccgtcgg tgccgcgcat
     3001 gatggtctcg agcacgccgt tgccgggtgc gccgtcgtcg gttgtcgacg ccgccccgcc
     3061 actcgataca gtgcacgacg tccgcacggg cggtggcgtc ctcaagacac tcgcggtcct
     3121 cgtgctcctg ttcgccgtcg gcatcggtgc cggtctctac gtgacgcgcg atcggtcgtc
     3181 gacggattcg caggcgatcg cgccacaaga tgcagagctg caggtgccgg cgaccgcgcc
     3241 gatcgatgct gcggccgcgc ccgagccgcc cgacgcccgc gagatcaccg tcgagaaggg
     3301 cagcaatgcg gccggcagcg gaagcgcgac gccgcatgtt caaccgccac cgaaacagcc
     3361 gccgccgaag aagccgccgc cgaagaaacc gccgccgaag aagccaccac cgccgccgca
     3421 aggctccggt gcaggatcgg cgacgatcgt gaagccgccg ccgaagtgcg acccgttcga
     3481 caacaagaac ggctgcagct agtcagctgc cgcaccagcg attgctgccg atcggggggc
     3541 agcctcgcag cgcgaacacg tccatcgtca tcgcgacgcc gacgtgaatc agcacgccgc
     3601 cccagatcga cttcgtccgc atcgcgaccg tgccgagaat gagaccggcg ccgatcgcgc
     3661 cgagcgtctc gggcagaggc ttgccgtagt ggatcatgca gtacggcacg atcatcacga
     3721 agatcgcgtt cgagccgagc gcgcggcgga gcccctgcag gatgaatccg cgaaagaaga
     3781 tctcgagcga gagaaactgc gcggcgtaga gcgcttccca cgaccacaga tcgaagtgcg
     3841 agcgattcgc cattcggtag aacggatacg tgtggcggaa cgcttgtgtc gttgacgcga
     3901 tgatcaccgc cggcaagatc agcgcgaaca tcatcacgta cagccagagg tgcgagaaga
     3961 accccttcgg cgagacgtgg taatcgcgaa tgcgttcgcc gggcatgagc gccacggcag
     4021 tccacggcat gatcacgtag ccgagcacgc gccaacccga ccaccacgcg aagcccttga
     4081 gctcgtaata cgggtccggg ccgtgcgacg gaaaccagcg ctcgaactgc ccgcgatcgc
     4141 cgatgtactc ctgcagcgtc agcgagagcg cgacgacgac gaggacaacg aggatgcgcc
     4201 aatcgaagcg acccgcagcc tcgggcggac gatcggtctc ggcgtcgatc gcacgccact
     4261 gatcgacgga gatgtacttg aaccacgcga cgaacgtcgg gtcgcgaaag atcagccaca
     4321 gcatcgcgcc cgctgcgatc aatgaggcga tgatccgcac gagcgtcgcc gacaggatcg
     4381 acaccgccgg caacagttgc gcgtagtgga tgagcgtccc catcacggcg gccatgccga
     4441 tgagctgcaa cagcttcggc cagctctcgc gaaaccatgc gcgcgcgtat cgcggcatca
     4501 gcacgccgac gatcgggatc gccagcagga gcacgagccc gatcttccag acgagctcga
     4561 atgtgaagcc gctgtcgtga tgagcggcca ttacgacttc agcttgcccc cagcgccgac
     4621 gacgtctgct gcacgacgca gacgacgacg cgcgatctct ttaccgagga cagccatcac
     4681 gtcgaacagc ggtggcgacg cggtgcggcc cgtgatcgcg atgcgcagca gcatgaaggc
     4741 cttgccgctg ttccagccat tggcctcgac ccagccgcgc gcgacctctt cgagcatttc
     4801 cttgctccag ccgtctttgg cctcgaagcg ctcgacgaga tcgagcagcg ccttcttgag
     4861 atccgtcgcg gcgacctcgg ggatcgtcat ctgcgaaagc acggccgagt aatcgagatc
     4921 gccggagaag aagtactccg tcgccgggat gatctcgtcg agcttcttga tgcgctcgcg
     4981 cacgagcggt agcaccttga gcagatagtc cttgttgacg cgccagttga tcagcgcatc
     5041 ggcgagctga tcgtagctct gctcgtggat gtactgctcg ttgagccacg tcagcttgtt
     5101 gagatcgaag accggcccac cgagcgacat gcgatcccac gagaagacgt cggtcatctc
     5161 ctcgagcgtg aacttctccc ggtcgccgcc gaacgaccag cccatgaggc cgaggaagtt
     5221 gagcagcgcg tgcggcagaa tgccgatgtc tcggtagtag ttgatcgaga ccgggttctt
     5281 gcgcttgctg atcttggtct tgtccgcgtt gcgcagaagc ggcatgtgga tgaactcggg
     5341 cgcatcccag ccgaacgcct tgtagagcag cacgtgcttt tgcgtcgagc tgatccactc
     5401 ctctgcgcga atgacgtgcg agatctccat caggtgatcg tcgacgacgt tcgcgaggtg
     5461 atacgtcggc atgccatcgg acttgagcag gacctgatcg tcgatctctt tggcttcgcg
     5521 cgtgaccttg ccgcggagct tgtcctcgaa ctcgatcggt cccgacagcg gcaccttgag
     5581 ccggatcaca ttgggctcgc ctgcggccgc gcgctttgca ccctcggcgg gatcgagatc
     5641 acggcagtga cggtcgtaac cgagcgtgcc cgccttcgct gcttgctgct cgacgcgaac
     5701 cttcgcgagg cgagcctcgg tgcagaagca gcggtatgcc tcgcccttcg cgaggagcat
     5761 gtccgcgtgc tccttgtaga tcgcgctgcg ctcgctctgc cgatacggcg cgtagggacc
     5821 accgacgtcg gggccctcgt cccacgacag gccgacccag tggagcgcgt cgaagatcat
     5881 ctgctcggag tccgatcgcg cgcgcgtttg atcggtgtct tcgatgcgaa ggacgaactt
     5941 gccgccgtgc ttcttcgcga agacgtagtt gaagagagcg atgtacgcgg taccgacgtg
     6001 cggatcgccc gtcggcgacg gtgcaatgcg cacgcggacc ggcttcatgg tgggaccttg
     6061 taacatggcg tgataccgtg aggtagatgc tcagcttgct cgtggcgacg agccttaccg
     6121 gctacgtcag cgacgttcag agtcactgga ccgccgacgg gacgcgcatc atcaccgaag
     6181 cgacgctgca caccggtgcc ggcgacgtga ccgtctcgca gtttggcgga accgcggtcg
     6241 atccgcaaac cggcgagcgg ctctcgatgc gcgagatgcc cggtcccgag ccgctcctcg
     6301 acgggatgcg cgtcgcgatc tcgccatcat tcgcgatgga taactcgtgg cgcatgcatg
     6361 tcgtcgtcga cagcatgaag gtcctcgatt atccggcggg cttcgtgcgc accggtccaa
     6421 cggccgcggg tcactatctg tattgggaat ccggctgcgt gttcatcacg gtcgattcag
     6481 acggcaccaa agagattgcc ggcgacaacg aattcccgct gatcagcgaa tcgatcgcga
     6541 cgtggaacga caacacagcg agctgctcgt acatccagct ccgcgaagag ggcaagaagg
     6601 ccgtcgagac cggtcgcgat cgcgtcaaca tcatcaagtt tcgcgatcaa tcgtggtgtc
     6661 gcccggcgac gaaggacgat ccagcgcgct gccatcctga tagcgcagca ggcatcacga
     6721 cggcgacgta cgtcgacgat tccggcagct cgcgcgacgg cgcgatcgtc gattcggacg
     6781 tcgagctcaa tggcgtgaac ttcgcgatct cggtcaacgg tcagacgcta ggcacggagc
     6841 cgtgcacgtc ggagctcaag aacacgctca cgcacgagct cggtcaccta ctcggtctcg
     6901 agcacacgtg cctcgcggcg ggcgacccac cgcgcatcga taacaatggc gccgccgtcc
     6961 cgctgtgcac gcaggcgatg agcaacatga ccatcaccgc cgcgacgatg ttcaactacc
     7021 aggcgtgcgg cgaggtcacc aaatcgaccc tcgagcccga cgacatcaac gcgatctgca
     7081 cgatctatcc gatagcccaa gatccaggca cctgcgagca cgtcggcgac ggcacgggct
     7141 gctgcgacag cagtggcaat ccgggcggtt ccctgctgct agcgctcggc gtgttgacgg
     7201 gtttttggcg gcgtacacgc cgggcgcgct agaatttcgc gatgaccggt ccggtatgcc
     7261 cgtcctgcgg tgtcgcggta atgccgggct acgcgcgctg cccgaagtgt cgcaagccgc
     7321 tgccgcgtcg caagcagacg gtgatgggcg gaaccgccgt cgattcgacg agccgcggtc
     7381 cgttgctcgc gatcctcgcc gccgcgatcg tcggtgccgg catcatcgcg tatatcggcc
     7441 tgcgcagctc gcagagctcc agtgcggcgc cgcgggccgc gactccgacg cagcagacgg
     7501 acacgacggc gaacgacgtc gtcgagtcga acgttccgga ccagcccgag acgcgcacga
     7561 acacgccggc ggcgccggat cccagcgccg tcgccgatcg cctcgagaaa gcgctcaagc
     7621 gcgagcgcct gtggtcgacg gtcagcgtcg agggcggccg catcgatgtt cgctcggggt
     7681 cgtgcagcga tccgaagatg gcgggcatcc tcgatgggag ctcgcaatcc ctgaagtcgg
     7741 ccggcttgac ccggctgcgc tgcctggagc agagtggcca ggtcgtctcc gatcgtgacc
     7801 tctgactccg aaccaccgcc gagcaagccg caccgcctga tcctgccgct cgggatgcgc
     7861 gtgctcgtcc aggtcattcg cgacgaggag cgcaccgacg cgggcctcta cctgccggcc
     7921 ggcgcgaagg aagcgcaaga cgacgcgcta tacggcaaag tcatcgaggt cgcgcgcgat
     7981 cgaccaacga cgaatgaagc gaccgagaac gtctccggcg tcccgcatgg tgcacacgtg
     8041 ctgttccgca aagacgccgg cgtgcgcgtg ccgtgggacg accggcttcg cgtgatcgac
     8101 gtgaaggatg tgctcgcgac cgtcgaagaa gtccgcgaca gcgaagcgca ctagcgtcgc
     8161 cgttcactag acctacgaga cttccgtggt accgtaacgg ccaacctctc ggaatctcat
     8221 ggacgtccgc tgcgagaagt gccagaccga gtacgagctc gacgagagcc ggctcaagcc
     8281 aggtggtgtc actgtcaagt gcaccaactg cgggcacatg ttcaagatcc gcaagcgggc
     8341 gaacacgaac gcgggcgtag cgagcgcgcc agccgcgaag gctcagccga ctccggcgcc
     8401 accgccgccc gcacctgcgg ccgcgcagcc ggcaccggtg agcgatcgca gcaagcgcgg
     8461 cgatgccgaa gcgcccgtga acatcgcgga catgccgtcg ggaccgcagt ctgcgtcggc
     8521 ggagcggcag tggctcgttc gcatggagaa cggcgagcaa aagacgtgtc gcgagctcgc
     8581 gacgctgcag cagtggatcg tcgctggcat cgtctcgcgc gagtcgttga tctcgcgcag
     8641 cggcaagaca tggaagcggc tgggcgacat cggcgagctc ggccaatact tcgtgatcgc
     8701 cgacgaggcg cgcgcgaatc gtgcagcgaa gccaactggg ccgtctgcga agggcgctgc
     8761 cggcgctggc acgatgctcg gcgtcggtgc gatgcggccg agcacgaatc ccgacgacga
     8821 tctcgagcag cgctcgaccg gcaacttccg cgcgcggccg ccgacgccgc ctcctccgcc
     8881 gcctgcatcg atgtcgggct cgggctcgaa agccaaccca atggcgcaga ccgagctcgc
     8941 gccgtcgggc ccgacgaagc gaccgccgac gaatccaccg ccaccgccgg tgtcgaagcc
     9001 aaagacgatg gagccgccgc ccggtcgcgc gaccgccggg tgggcgagca acgatgtcaa
     9061 ggcgagcgag tcgatggcgg cgatgccgca aggtccgcgc ggcggcaagc tgtcggcgag
     9121 ccccgacgag cccgcatttg ccggcaaggt gcgcgtcacg ccgagcgatc gctcgacgtt
     9181 cgacgggcgg gtcgtcgccg aggacgatga cgagttttat cccgcgtcgc gcggctcgcg
     9241 cgcaggcatg tggatcgcgc tgatctcgct cctcgtgatc ggtgcagcag ccggtgcggt
     9301 ctacatgttc gtcttcaagg cgaagcaatc gccgacggcc gagacgacga agcaagatgg
     9361 cggtatcgcc acagccggcg atgcgagctc ggttgtcgtc gcgcccatcg tcgacgcgca
     9421 agaagtcgct tcgagcccgc tcgatacagc gcgcgccgag ctcgtcggcg gtgtgcacac
     9481 gcgcatgaag tcgacgctcg acggtctcgc cggcaaagac gatccagcgt cgctcgcgat
     9541 gcgcgcgcgc ctgatgaccg cgatggcgca aggcatgaac gatcgcgccg gcttcgtcga
     9601 caaagccgaa gcggacaagc tgcgcaaaga ggcaaaacag atcgtcatcg atgcagcgcc
     9661 gctcgcccaa cgcgcggtcg ccgcgcaagc cgacagcgtc gacgcaaacc tcgcgatggc
     9721 cgacatcatg cggctgcagg ccaagcccgc tccgtcggtg aagcgctaca tcaccgcggc
     9781 caaaggcaaa ggcgataaga gcgccgccgc atcggtcgca ctgtccgaag cacagctcgc
     9841 gattcgcgac ggcaagctcg ccgacgcaac gaccgcgctc tccagcgccg aggcgccagc
     9901 cgacgacatg cgcatcaagc tcgcgctcgc gctgatcgcc tacgcacaag ccaagccggc
     9961 cgacgccaaa gcccacgtcg atcagatcct cgcgacgcag ccggatcacg acgcggcgct
    10021 cgcgatgtac aagaagctcg agacgacggt gtcgagcagc gacccgcttc cgcccgaaga
    10081 cggcagcggc gccaaaccga acaacgcggg cagcggctcg acgacgacaa acaacggcac
    10141 gggcggcaac ggcggcaacg gcggcaacgg cggtaacggc ggcaacggag gcggcaacgg
    10201 tggcggcggt ggcggcggcg gcggtggcga cagctacgac gcgctcctcg ccaaagcgaa
    10261 caagctcgcc gaatccaact gctcggccgc gatgccgatc ttccaaaagg cgctcgacca
    10321 aaagccgaac ggcgtcgagg cgctcaccgg caccggctac tgccatctcg atcgcaagga
    10381 gttctcgagc gcgttctcga agttccgcgc cgcgctcctc gtctcgccgc gctacgagcc
    10441 cgcgctcggt ggcgtcgccg aaacctatca acgccaaggc aacaagcccg ccgcgatcga
    10501 ggcgtggaac aagtacctcg aggtgttccc cggctccgcg aaagcgaaga agcagctcga
    10561 gatcctcggc gccgacacga ccggcggcgg cacgaccaag ccgcccgaag gtggtggctc
    10621 ggcgtccggc ggcgacacga agcccgcagg tggtggcgat acgaagccgg cgggtggctc
    10681 cgaaacgcca ccagcgcccg cccctacgcc tgcgccaacg ccggctcctg cgacgccgta
    10741 agcgcggttc gctcgcgcat cgccgcgccg atgacgtcca cgtgacacgt ccacgtcctc
    10801 gtccacgtcc acgtgacacg tccacgtcca cgtccacgtg ctcgtgctcg tgctcgtgct
    10861 cgtgctcgtg ctcgtgctcg tgctcgtggg aaccggattg actagaggca caaggacctg
    10921 gagggtctga accaggtgaa gaccgtcgag cacgcgttcc tcgagaccct ccttcgtacg
    10981 ctccgttcat cccctcgtgt ctctgtggtc gatccgatcc agtgcattcg cgacgacggc
    11041 cgcgcagtcg cggtatgaag cggccatgcg tgctgctctc gtcgcgatca tctgcctcgc
    11101 tgcctgcaag tcgaacccat cgccactcga tcacgcgttc ggcagcggct cgtacgcggg
    11161 cccgaccgac gatccgtggg ccgcgacgct cggcacgaag aacgaggacg gcacgagcag
    11221 ccgcagcggt gacggcggct tcgatctgca gggcattctc gagaaggtca aagaggcgat
    11281 cgatacgccg gggccgtacg agtcgccgaa gcagacgaag gactttgcgg tcgacaagcc
    11341 gcactggggc gtcatgggcc tcgacggcga gatcgtcgag cgcgaggcgt tctcgatcac
    11401 gggcgggggc ggcaccgagc tgcgtcacct cgtcgtgcgg ctgcgtgcac tcgcgaagaa
    11461 cgagcagctg cagggcttgt tcctgcgcat cgagcccctc tcgatcagcc tgccggattt
    11521 gatggagctg cgcgctgcga tgcatgactt ccgcgccgcc ggcaagaagc tctactgcca
    11581 caccgagtcc gcgatgaacg cgacgtacct cgtgatcgcg gcgtgcgatc gcattgcact
    11641 cgcgcccctc ggtgacatcg ctctgacggg accgtcggcg atgccgatcc acgtcaagcc
    11701 gctcctcgac aagctctcga tcaccgccga cttcatccat gtcggtgcgt acaagggcgc
    11761 cgccgagcca ctcacgcgcg atgcgccatc gaaggagatg gaggagacgc tcggcgccat
    11821 cctcgaccgg cgttaccaga cgatcgtcga cgtcgtcgcg gcggagcgca agctcgaacc
    11881 ggcgacggtg aaggcattga tcgacgaggc actgtttccg gccgacgtcg cgctcggcaa
    11941 gaagctcgtc gacgaggtca cgccgtggga gcaattccgc gacagcgtga aggcgccgtg
    12001 ggcgaagatg gagatcgagc ccgacgacaa agatcagctg caagcgatga tgaagctcgc
    12061 gcggtttgtc ggcgcgatgc cggcggagcg gccgttcggc gatcacgtcg cggtcgtcta
    12121 tgcgctcggc gatatcaagg acggcgacgg cgatggtgtg atcggcgcgc ggcaagagat
    12181 cgcatcgcac acgctcgtgt cggcgctgcg cgcgatcgcg gcggacgatt cggtgaaggc
    12241 ggtcgtgctt cgcatcgatt cgggcggcgg aagcgcgcaa gcatcggagc tgatctggac
    12301 ggcggtcgag cagctgaagg cgaagaagcc cgtgatcgtc tcgatgagcg acgtcgcggc
    12361 gagcggcggt tactacatcg cagccggcgc gacgaagatc ttcgcgcagg aagacacgct
    12421 caccggatcg atcggcgtcg tcggcggcca catcgcgccg gccgccgcac tcgcgaagct
    12481 cggcgtcaac acgttcccga tggggcgcgg caagcacgcg acgctcatgt cgagctttca
    12541 gccgtggacg gacgacgaga agaagctgat gcagacgcac atggaggcgg tctacaaggt
    12601 gttcgtgtcg cgcgtcgcgg cgggccgcaa gaagaccgcc gagcagattc agccgatcgc
    12661 gcagggccgc gtgtgggtgg gcacgaaggc gaaagagctc gggctcgtcg acgaaatcgg
    12721 cggtctcgat gcagcgcttg cggaagcgcg caagctcggc aaggtcgacg aagcgatcga
    12781 cgtcgaggtg tatccgccgg caccgacgct gcgcgacgtg ctcgccggtt ggggccaagt
    12841 gcacacgccg tttggcctgt cgatgcagct tcagctgcta gaccccgaga ttgcgaaggt
    12901 cgccggccac ctgctcgcgc tcgtcgatgc tttccgcacc acaccgattc aaacgatcgc
    12961 tatcctcccc gccatccaat gaagtgcctc gtcctcgtcg tcgccctcgc cgcctgctcg
    13021 tcgcagcaaa cgaccgcgcc gcagcagaag tcgccggcac ccacggccgc gccggtcgcc
    13081 cctgcgaatg ctggccgcgt cgagaccagg cacttccaga gcgctgcgct cggcgtcgag
    13141 aaggactaca tcatctatct gccggcgggc tacgacgcgt cgacgaagcg gtatcccgtc
    13201 ttctactacc tgcacggact cggcggtaac gagacgaact ggaccaacac aggcgagctc
    13261 gcgcagtccg ccgacaagct cggcctcgaa gcgatcgtcg tcatgcccga cggtgatgac
    13321 ggcttctatg ccgatagcgc cgcgccgatc gactacgaca cgtgcatgaa ggacggcacc
    13381 ggcctgttcc ttcccgggat gcagaatcgc caacacacgt gcgtgaagca gcggaagtac
    13441 gagacgtaca tcatcaaaga tctcgtcggc gagatcgacg cgaagtaccg gacggtcgcg
    13501 tcgcgcgaag gtcgtgcgat cgccgggctg tcgatgggcg gcttcggcgc gctgcagctc
    13561 ggcatgcggc atcccgatct gttcgcggcg gccgcgagcc actcgggcgt cgacgcgctg
    13621 ctatacgcgg gcccgtatcc gtacgagaag ggcaaggtga agctcatcac gcaggaagag
    13681 atttcgcgct acggcaaggg caatcccgac gtcgagcaga tcggcctgtg gatccagatg
    13741 atcttcggca aggacatcgc ggtctggaag agctacgacc cagcgacgct tgccgagaag
    13801 gtcgagccag gcaaaccggc gctttacctc gactgcggca ccgaggatgg cttcggcttg
    13861 cagaacggcg cgcaatacct tcacgacatc ctgagcgcgc gcaacatcga gcacgcgttc
    13921 ttcctggggc caggcccgca cgacatgcgc ttctggcgac cgcggctccc cgagagtctg
    13981 aagttcctgc gcgatcacac cgtcaagccg acgtaggatg aacgcgtgaa gctggtcggg
    14041 cgggaccggg agctcgaaca gctccgcgac gtgtgcaggc aggcgcgcga tggccgcggc
    14101 cgcgtggtgg tcgtgtccgg agatgccggc ctcggcaaga gcgcgctcgt cgaagaaatc
    14161 gcgaacgagc tcgcgccggc gttcaccatc acgtggggcc gcgcgtggga gctcgcggat
    14221 gcgccggcat atttcccgct atggccgtgc ctgaaatcga tcggcgttgc gttcgatggc
    14281 cgcggtgacg agaccggtgc cttcgagctg tgggagcacg tgctcgaagc gctcgtgcga
    14341 gccgcggcga cgaagcctgt gatgtggatc gtcgaggatt tgcatgtcgc cgacacgcag
    14401 tcgctcgact tactcaactt cctcgctcaa gcggtgcgcc cgctgccggt gatgttgctt
    14461 gcaacgacgc gcgcgcaagc agccgatgcg gcgcacaagc gcattgtccg gctcgctcgc
    14521 atgggcatcg agctgcgact cgcgccgctc gcacgcgaga acgtcgatgt gctcgcgaag
    14581 cactggctcg atcgcgacct ctctgccgac gagctcgccc agctctcgga gcgaaccggc
    14641 ggcaatccgc tgttcgtgat cgagtgcgct cgcgccggaa agggcaacgg caagcatctc
    14701 ctcggtgcgg tgccgccgac ggtggcagtc gtcgtcggcg agcgcgtcga gcaactacct
    14761 gcggccacgc gcacggtgct cgcggatgct gccgtgctcg gcaacgagct gaccgccgcg
    14821 acgctcggcg cgatgacgga caagctcccc gcgcgcgtga tcgatgacct cgcgcccgcg
    14881 ctccgcgcgg gactcatcga agagctcgca cctggtcgct tccgcttcca ccacgcgctc
    14941 gttcgagacg cgatcgagga tcaaatggcg ccggcccagc ggcgagccgc acatcagcgc
    15001 gccgaagccg cgttgtcgtc gggcatcgat tcaccgcgcg cgattgtcga gagcgcacgc
    15061 catgcgttgc tcggcgtcga ggccggtcgc gaagcgcacg tcacggacat cgtcgagcgc
    15121 gcgctgtctg tgctcgagga gcaaggcacg ttcgatcgcg catcgcagct gtaccgccag
    15181 ctgcgcgacc tgcgcgcgaa cggcgtactc cccggcggca cgccgagcga cctcatccgc
    15241 ggcgcagcga tcgcacgtcg cgcagggcag ttcgcgtata gccgcgagct gtcgctcgaa
    15301 gctgcgaccc gtgcacgcgc caccggcgac accgaacagc tcgcatatgc ggcgcttgcc
    15361 gtcggcgcgg agatccaccc gggcgtcgtc gatcccgtcg tcgtgcagca cctcgaagag
    15421 gcgctcgcga aggcgcccgc tggcccactg cattgccggc tcaccgcgcg cctcgccgca
    15481 gccatgcagc cgcatgccga tccacgtgtg ccgatcgaca tggcgcgcga ggcaattcgc
    15541 gaggcccgca agctcggtga tgcggcgctg ctcgaagatg ttctctacgt cgcgacgtca
    15601 gcgatgatcg agtacgtcga cgccgagcag gtgcgcgatc tcaatcgcga gcacctcgcg
    15661 ctggcgcgta cgcacgctga tgccgagcgg cagattcgcg cgtatacgcg gctgctcttc
    15721 aacgaaggcg agctcggcga cttcgcgatg ttcgacaagc tcctcgacga gatgatgctc
    15781 gtcgcaaccg cggcgggaca tccgcgcgta acctggcgcg cgttgctgat gtcctcgatg
    15841 agggcgatcg caacgggaca gttcgccgaa tcggagcgct tcatcagcga ggtcgagcgc
    15901 gcggcgcagc tcatcgatga cctcggcctt tcgatgtcac ttagcgcgca tcgccacatg
    15961 cgcgcggtcg atctgcatcg tgaaacaacc gtgcaacagg ccatcacctc gatcgaagca
    16021 tcgatcggcg cgaacctgcc gggtcgtgcg ttcatcgtgc cgatgatcaa gggcatcgcg
    16081 atgtcacggt tcggcgatgt cggcgccgca ggacgcgcgt gggccgcgct gccactcgac
    16141 gatctgttct ctcgcggcgg cggcaccgtc gggtggctct cggtcatcgg cgaaatcgcg
    16201 gcgtgcgtcg gtaccgacga gcagcgcaag aatatcctcg agcgcattca gcctttcgct
    16261 tcccgtcacg tctacttcgg ccacacgccg atgatctacg gaggtcctgt ccgccgcgtc
    16321 atcggccttc tcgagctgtc gctcgggcag aaggcagccg gcgagaacag cctgcggcgc
    16381 gccctcgacg tctgtcggac gaacgggttc gctccgtggg tcgcacgcat ctcgttcgag
    16441 ctcggcgaga tggcagaagc ggcgcgcctc gccacgaatc tcgggattcg aggtctcgcg
    16501 aagcgcgcgg cggttccgga ggccgagtcc gtcgctccac cgacgaagcc ggcgcttgga
    16561 gtctcacgcg aaggcgagct gtggcgcatt tcgtacggcg tgcgagtcgc gcgcgtgagc
    16621 gatgcgcgag gcatggagct gatcgcgaag ctcatcgaac gcccgggaga agacatgcac
    16681 gtcctcgtgc tcgcgggcac gggtggcgaa gcgatgaccg acagcgatgc aggcgatgcg
    16741 ctcgacgagc gggcagcgaa gagctatcgc gagcggatcg ccgcgatcga tggtgagatg
    16801 ccagtcgcga aaggcgagcg cgcgaatcaa ctcgcacgcg aacgtgcatt tctccaaggc
    16861 gagctcgccc gcgcgtttgg cctcggctac gcgcctcgtc gcgtcggctc gatgtccgag
    16921 cgcgcacgcg tgaatgtcca acgccggatc aaagacgcgc tcacccgcat cggccatcac
    16981 gacccggtca tcgctgacta tctccgcagc gcgattcgga ccggcacgta ctgcacgttt
    17041 cgtccgtaag tctctggaac acctgagctt cgcgtccgga acattcgttc catccgacgt
    17101 cacgcagagt gggcatggaa tcgatcatcc ctgccctgtt cccgctgacg tacttcgcgc
    17161 tgctcctggc cgagcgcctg cttggaggtc gccgcttccc gaaggtccgg tggtggatcc
    17221 ccaagggcat cctgttcttc ttcatcggcg gtctcatcaa ctcgacgctg ccggtggcgc
    17281 tcgcgcgcgt gttcgcgggt cacacgctgc tggatctgtc gagcctcggt ctcgcgggca
    17341 cggtggtcgc ggtgattgca tcgacgttcg tgtcgtactg gctgcaccgc acgttccatc
    17401 gcttcagcgg gctgtggcgg tggacgcacc agatgcatca cagcgctgaa cgtgtcgaca
    17461 tggccggctt tggctatagc catccgttcg agctgctcat cgcggttgga atgacgccgc
    17521 tcgtcgcgtc gatcatcggc gtcgcgccag aagccgcgat gctcgccggg tacctcacgt
    17581 ttgtcctcgg cctcgtcgag cacttcaacg tgaagacgcc gcaatggctc ggctacatcg
    17641 tgcaacgtcc ggagatgcac tgcgtccatc acgagcgtgg cgtgcatgcg tacaactacg
    17701 gcctgcccct ttgggacatg ctgttcggca cgctccgcaa tccgaaggcg tggagtgggc
    17761 aggccgggtt ttgggacggc gcctcgaagc agacgttcgc gatgcttgcc ggccgcgatg
    17821 tcgcggtacc gccgcacgtt gccgccgtcg cgaatgcagt ccccgagcga atcgccgcct
    17881 agttctgcgg aaccatcgcc atcagcgatc gcagcgcatc gcgagctcgc tccacgtgct
    17941 ccgctgccga gctgaccgct tggccgaccg cgacgaccga atccgattga tccatcatcg
    18001 ccgtctcgtc gctcgccgca cgcatgttcg cgcgcagcga atcgatcgat tcttcgagca
    18061 cgctcaagta ttcggcgaac tccatcggga tgctcgcgcc ggtattcgtc ggctcgtctt
    18121 cgtttgcgcg acgctgggct gcgagcttct gctcggactc ggagagctcg cgccgcatgc
    18181 gctcgccgtc cttctccatc ttcatgacct tctgctcgag ctcggccgca cgcgcgacga
    18241 gcgcggtgta gcgcgagagg tcggcttgtg cggtgacctc tggatcggcg cggccaccgc
    18301 cgacggcagg aacccggcga ttcgtcgtct cgctgtcgtc gcgtaggtcg cgcagctgcg
    18361 attcgaggcg atcgcgctcg agcgtgatct gcgtgacctt cgccttcatc tcgcgcagct
    18421 cgcgctcgag accggcgatc tcgcgctcgg tgttcgccgc aaactggcgg acgttgtcgg
    18481 cgcgatcgac ctcggcttgc agctcagcga cgcgagcctg cagcgcagcg acttcgccca
    18541 cgtccgagcg ctcttgcgtt ccctgcagat cctcgagctt gtcctcggcg tcctgcgcgc
    18601 gacgaatcgc atcggcgagc ttcgtctccg catcacgcgc acggcgctcg gcttcgacga
    18661 tccgctcggc cgcttccttc gcccgcgcct cgatgtcctt ggcgcgagtc tcgatgtcgc
    18721 gagcgcggcc ctcgacctct tgcgcgcgag cctgcgcggc ggccatctga acctcggctt
    18781 cacgcgcacg cgcgtcggca tcacgcgcca tcgccgtctg cgcttcgacg cgggcgtcgg
    18841 catcgagcgc acggccctcc gcgtcgcgcg ctcgcgcttc cgcctcgtcg atgcggcgct
    18901 gcacctccgc ttcggcggcc gggttcttcg cctttgcatt ctcgagatcg cgctccatcc
    18961 acgacagctt ctggagcgat tcgtcgagct ggcgctgcag cgcattcgcg cgaccgatcg
    19021 cgaggtcggc ggcctcggcc tttgcagcga gcggctcgag cttggcctcg gcgtccttcg
    19081 cgcgtgactc ggcggcttcg atcagcgagc gatcggtgtg gaccgtcgtg gtgtattcct
    19141 cgacggagcg ctcgagatcg ccgacgcgcg cgcgcgcttc ggtcagcgcc gcctccgccg
    19201 ctgcgcgcgc ttccttgacg gtcttcagct cggtctccag cgcatcgagc cggcgttcga
    19261 gacctgctgc ggtcttcgat gcggtcttgc cggccgcctt ggattcctgc agctgctctt
    19321 cgagcttctt gatgcgctcg cgcgcttctt cgatcgagct cgatgccgca gtctgctcga
    19381 ccgcagcgcc ggcctcgaga tcctggatgc gatcttcgag ttgctcgacc tgcacttcgt
    19441 actccgcgac cttgccctct gcgagctcga gccggtgctg cagacctgcg agcgtcgtgc
    19501 gcgcttcgtt gagctcgcgc tcgagctggg ttttgcgatc gacgagcgac atcgccgtag
    19561 accgctccgc cgccagctcg cgcgccgtct gatcgagctt cgcttcgagc tcttgcttga
    19621 ccttctcggc ctttgcgacg agctctttct ccgtcgagcc gagcttgtcg acgagctcct
    19681 tctgcagctg ctcgagcttg tcgccgagct ctttctcgtt ggcgatgcgc gccgcttccg
    19741 cagcggctgc ccgatcctcc gccgccttga tcagcgccgc agcgtcggct gctttcttct
    19801 cgccctcggc cgccttcttg tgcgcgccgt cgagctcgcg cgtcaccgct cgcgacttcg
    19861 agtcggaatc gaccgcgcga cgcagcgcct cggcgacgtc cttcgccatc gccttcgcca
    19921 tcgtgtcggc ggcacgcgcg cggttctccg cttcttccgc gcgagccagc gattcggcga
    19981 tcttcttgtc agccccggcg gtttggctgc gcgcttcttc gcgctccttc ttcgcggccg
    20041 ccatctcggc ttcggcgccc ttcagttgct cgaggcgcgc ttccagctct ttcaccttcg
    20101 cattcgccgc ggcgagcttc tcggcctgcg cgcctgcgtc cgcctgcagc ttcgcttcga
    20161 gctcacctcg cgcggcgcgc gcctgctgca gctgcgcatc ggctgcgagc gtggcgtcct
    20221 tcgcctcgat cagatccttc tcgaggtccg ccgcccgcgc cttggctgca tcgcgtgctg
    20281 cgtttgcttc cgcgagctcc gcggcgagag ccgtcgcgcg cgcttccgct gcctgcaccg
    20341 gcgctgcgcc ctgaccgagc cgcgattcga gctgcttgat cttctcgttc gcggtcgcga
    20401 gctcgctgcc cgctttcgac ttctccgccg cgatctccgt cgcgagcttc gccttctccg
    20461 cagagagctc ttcgatcttc gactgcagct ccgcggcctt cgtctcggct ttcgccgcct
    20521 gcatgttcgc gtgctgcagg ttgcgctccg cgccttgcgc gcgaatctgt gccgatgcca
    20581 ggtccttctc cgccttctcc gcgcgggccg tcgacgtgac gagctggtct tcgaggatct
    20641 tcgacttcgg ctccgcagcc gtggcatgca cctgcgcacg cgccgtgatc agctcgttct
    20701 cgagccgcga gatgatcgcg tcctgctcgg ccagcttctc gacggcggtg agcccagccg
    20761 acgtcgtcgc gacgatcttc tcctcgcgtg ccttcgcgag ctcgacgcgc acctgctcga
    20821 ggtccgccac gctcttgcgt cgcagatcat cgagctcggt gcgcagcgtg cgctcttctt
    20881 cctgcaggag gttctgcgcc tcccgcatga tgcgaatctc gtcgtgcagc tcgccgacgc
    20941 gctgcgcgaa tgcgttgcgc tcttcctcga cggcgacgcg cgccttgcgc tccatctcga
    21001 gccactgcgc gagcgtgccg tgcaggccca tcggcggcgg caacgtcccg ctcgccggca
    21061 gcagccggat catcaagctg ccgatcaaga tgatctcgcc gaccggcacc ggcgcgcgct
    21121 gcgtcttctg cggaccgacg tagatgccgt tgctgctacc gagatcctcg acccacagcg
    21181 ttccctgatc gacgaagaag cgcgcgtgca tgcgcgacac ccgaggatcc tcgctgcgga
    21241 tcgtgcaatc gggcgcacgg ccgacggtta ccggctcgtt ccccaacgag aacgagcggt
    21301 cgacaccgtc gacgtccgta tagacgagcg tagccatccc ttagggattg ctacggtatc
    21361 acgcgcggga cagcgtcgac acgagctggt cggccgcttc taacgcgcga tcggtatcgt
    21421 cgtcgagcag gaggacgtgg cccatcttgc ggcccggcgc cgcgaggtcc ttcccataga
    21481 ggtgcagcct cgcctcgggc cgcgccagca cggtgtccca cttcggtggg ccggagtgcc
    21541 agagatcgcc gatgaggttg accatcacgg atccgctcat cgcggtcgga tcgccaagcg
    21601 gctgcccgca cacggcgcgc acgtgctgct cgaactgcga cgtcacgcag gcgccccacg
    21661 tgtagtgccc gctgttatgc gtgcgcggcg cgatctcgtt gaccagcagc tgcccgcccg
    21721 cgagctcgaa catctcgaca gcgatcacgc cgacgtgacc gagctgcttc gcgacggtca
    21781 cgccaatctc ttccgcccgc ttctgcgtgg caggcgtcat cggcgcaggc gcgcgcgtcg
    21841 tgtgcaggat gtgccggcgg tggacgttct cggcgatcgg atacgtgcgc acctcgccgc
    21901 gaatgccgcg cgccagaatc accgaaatct cgcgcttgaa ccggacgagc tcctcggcaa
    21961 cgaccggcgc gccgcgcagc ttcgcgaggt cggcgctcgc gtcggaactc tcctcgatgc
    22021 ggacctggcc tttgccgtcg taaccgcggc tgcggacctt gaggatcgcg gggcgtccga
    22081 gctgcttgag cgccgcggca agctcggctt ccgtcgtcgc gggcgcccac tgcgcctgtg
    22141 gcaatccgag cttgtccaaa aactgcttct ggatctgccg gtcgttgatc gtgccgagca
    22201 cgctcgcgcc gggccgcacg ggcaccagct tctcgagctc ggcaagaata tccgccggaa
    22261 cgtgctcggt atcgagcgtg atgacgtcga cttgccgcgc tagatgcttc gccgactcca
    22321 gatcgtcaag cgctccgacg accacgccat ccgcgacctg cgccgtcgga caccgcgcgc
    22381 tcggatcgag cacgaccacg cgatagccca tgcgccgcgc ctcgatcgcc atcatgcgtc
    22441 cgagctgacc gccgccgagc acgccgatcg tcgagcctgg gccgagtggc ttcacttcgt
    22501 atccccgtcg tcgagcgcct gcgcgagcac gtcatcggtc tgctgcttgc gaaacacatc
    22561 gagcgcgagc gcgaccttcg gatcctgcaa cgcaacgatc gacgccgcca acagccccgc
    22621 atttgccgcg cccgcttcac caatcgcgag cgtcccgacc ggcacgccct tcggcatctg
    22681 cacgatcgac aacagcgaat ccagccccga cagcgccttg ctctgcacgg gcacgccaag
    22741 cacaggcaac cgcgtcttcg ccgccgtcat gcccggcaag tgcgccgccc cgcccgcgcc
    22801 ggcgatgatg acgagcaacc cacggtccgc cgcggtgttc gcataattga agagcttgtc
    22861 cggcgtgcga tgcgccgaaa caatctcggc ctcgtacggc acaccaaggc gctccagcat
    22921 ctcggccgcc ttctgcatgg tcggccaatc gctctgcgag cccatgatga tgccgacctt
    22981 cggcttcgtc gcggtcgccg tcgcggtcgc cgtcgccgtc gccgtcgcct tcgctcgttt
    23041 cggtttgctc atgggctgcg tgggcccgcc gcacggcgaa gccgatcgcc gacagcttcg
    23101 cccagcccca ccgctggagg caacgtagcg atcaccactt ccactcccat gccgtcgaga
    23161 tctcgcaacg ccgcatacag ctcacgcgcc atgccaggca gatcatccgg aagcggatac
    23221 gcacgcgccg cgatcggccc ccacgccgga aactccgccg caggtgccag cacggcaatc
    23281 cgctcgcgca ccatcgtcgc gcgcttcacc cggccgctcg gcgacgcctc cgtcgtcggc
    23341 tccggcttcg ccgacagctc cgccgcaatg cgcatgaccg tcgccggcac ctcgtgcggc
    23401 tccaccgcaa tgacctccgc atgcggcgca taatggctcg cgagcgtccc cggcgctgca
    23461 ggcgccgcct cttcgatctc atcgggctcg agcaacgctt cgccgaccat gcgctcgatc
    23521 gcatcgcgcg tcattccgcc cggccgcagc agcacaggcc gcccacgcga gaaatcgacg
    23581 atggtcgact ccacaccgac gctgcacgca ccgccgtcca aaatatagtc gacgtcgtcg
    23641 cccaaatccg ccgccacgtg atccgcggtc gtcgggctca ccgcgccgaa tcgattcgcg
    23701 ctcggcgccg caatgccgcc gccaaacgca tgcagcagct gctgcgccat cggatgcgaa
    23761 ggcacgcgca agccaaccgt atcgccgccg cccgtcgtcg ccttcgcgac gcgatgccca
    23821 cgcttcaaga tgagcgtcag cggtccgggc cacgccttgc gcgcaagtcg catcgcattc
    23881 tccggaatct ccagcgccca accctcgatg cgcgaatccg acggcagatg cacgatcagc
    23941 ggatgcaccg cgggcctgcc cttcacggca aaaatcttcg cggccgcctc gtcgcgcgaa
    24001 gcatccgcgc cgagcccgta caccgtctcg gtcgggaacg cgacgagccc accggctcga
    24061 aggacgcgga ccgcctcggc gatgtcggtg cctcgcttca tcggcgcagc ttatcagtgt
    24121 gtgctgtagt cggggaatga tgatgctgcg agcactcgcg gtcggggtcg acgactccga
    24181 agagctgcag tgcttcacgc tgggcttcgc ggaggaagca gacggcagcg gcccggcgct
    24241 gatgttccag cgcgcgtacg ctttcgacga acaggatatc gagctcggct tcgacaccta
    24301 ctgtctcgtg accgactcgc agtcgacgac ctacggcggc atcgagaagt ggcagctcgc
    24361 cgaaggctgg ctgcgcctgt cgctgtcgtt caaggcggcg gaggatctcg gcgtcgaccg
    24421 agacctgctg atccagttcg cgccggtgca catggagctc gtgcgctccg cgctcgcgcg
    24481 aatcctcgcg cccgtcgagg cacccgtggc ggcggacgcg acgcaaccaa aggccgcaaa
    24541 ggcgaagcgt gcgaaggcga agcgcgcgaa ggcgaagcgc gcgaaggcga agacgaagac
    24601 gaagagcgag gcgagggcga agacgaagag cgcacgccga taacggccac acggccgtcc
    24661 gcgccgcaga tcgacgtcgg tctcgtgccc acgtgggaaa agcagatcga atgggtctcg
    24721 tgcccacgtg ggcacaacaa atcgacatcg gcctcgcaga gaagcgacct cgatcggacg
    24781 tgccgcgccg tggccggcgg cgatgtcgcg gcgcgcgccc tggccgtcga gggccggggc
    24841 gcgccgctcc accgccggca cggcgctcgt ccccacatcg agaactagcc gaacagcgaa
    24901 cgaacatcgc cgcgcgactg aacatcgcgc gtcgtgccca cgtgggcacg gcagatacag
    24961 cagatcgaat cgctccgtca ccacgttgac cgttcgcaga gaagcgagct cgatcgaacg
    25021 tgccgcgccg tggccggcgg cgaagtcgcg gcgcgcgccc tggccgtcga gggccggggc
    25081 gcgccgctcc accgccggcc tggcgctcgt ccctcatcga ggacaagccg aacatcgccg
    25141 cgcgattgaa catcgcgggt tgtgcccacg cgggcacgcg accgggactt cgcgggtcgt
    25201 gcccacgtgg gcacgccaga tcgaatcgct gtcgtgcgca cgcgggcacg cgacctggac
    25261 gtcacgggtc gtgcccgcgt gggcacgaga ttcaattgcg ggccgtgccc acgttggcac
    25321 atcaacacat cgcgatcggc tcatgccggc gtgtgcacaa cgactggttc gccgggtggc
    25381 tagctcattc tctggcgcac ttggtggcga gcgcctggcc cggcggtgga gcggcgcgcg
    25441 gggcgccatc aaagcgcccc cgcgcgcgcc gcgatcccac cgccggccca ggcgcggctc
    25501 gcactttcta gtccgctctg tttttgatcg gtgtttgatc gcgagtggcg aatcgcgatc
    25561 gccgcaccat tgatgaccga cgacgcagcg acaatatttt gacaactttt cgcttgacgt
    25621 ctttcgagtc gcaccgagac gcgcatcgcg agacgcgccg gtgatcagtc attagaacgc
    25681 gcagcgcgcg atgtgctgca cgaattcagc gcgaacgctt tctcgtcgag aaattccaac
    25741 aaacgcacgc gtcgcgcaaa tcgcgagcgc cgaaaaacgc gtgctagtga aggaacatgt
    25801 tggggaacga acgagtcatc gataccagcc acaaaccgag cactgcgcaa atcgatcgcg
    25861 cacttcgcga tctcgcgcag cagcacgcag cactcgaagc cgatgaggcg cattggctgc
    25921 gactcgccga ccagcagaag atttggccga agctaggtta cgtccacgcg ctggagtact
    25981 tggaggacgt cttcggctac gcgcctcgaa ccgcgaaaga tcgtattcgc gtcgcgcgtg
    26041 agctcggcga cctgccgcag ctcgaagatg agcttcgatg cggcgagttg agctactccg
    26101 tcgtccgcga gctcacgcgc gtcgcgacgc ccgaaactgt cgagcgctgg atcgaacagg
    26161 cgcgcgggcg caggctacgc gatgtggagc agatggtttc cgggcgcaag aagggtgacg
    26221 acccagatga caagcccgac gcgaatctcg tgaagcacac ggtggtgctc gagctcgatg
    26281 gagcgagcat ggcgatctgg cgcgaaatgc aggctgtgct cgaagccgag ctcgagacat
    26341 cactcgatga tcgcgcgctc gtcctcgcgc ttggtgaccg aatcctcggt gctgcttcga
    26401 ctgctgttcg cgatcgcgcg ccttcgaacg ataacgagat cgacggcccc gacgccccgg
    26461 acgcgccggc cgcagccggt gcagccgacg cagcgtacgc acccgacgcg gtcgctgaca
    26521 cgagcgagcg cctgcgcgcg acgaaaacga ttcccgcagc aacgaagcgc cgggtgctcg
    26581 aacgcgacca aggtcgctgc caggtgccgg gttgtcgctc gaggaagcac ctgcagttcc
    26641 atcacatcga gtttcagtcg catggtggcg gccacgagga ctcgaacttg ctcctgctgt
    26701 gtggtggcca ccaccggttg ctgcacgacg gcctcctgac gatcgccgga cgcgcgccaa
    26761 acgcgctcgt cttcacgcgc aacggccagc gcctgcggag cgccgacgat ggtgcgcagc
    26821 cgcagctcag cggccgcgac acgcacgcga cgagcaagca tgcgccggtg cc
//
LOCUS       DATLBZ010000461        15671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1325091, whole genome shotgun sequence.
ACCESSION   DATLBZ010000461 DATLBZ010000000
VERSION     DATLBZ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15671
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325091"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VMZ53_23290"
     CDS             complement(<1..1308)
                     /locus_tag="VMZ53_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS31458.1"
                     /translation="MAKLIVISGEERQEFELAAFNTIGRHPDNTIQILDRIISKEHAQ
                     IQRSADGRFLLRDLRSLNGTFLRGERVSDHYLADGDEFTMGSTRIVFVDKPKADDALQ
                     RVTIAPGLTESHIRGRIQANTGDFMPERQIGDDKILRRDYERLRIGHELARAVGSELD
                     LDKLLPKILDKAFELVGADRGVILLMDEKGELAPRFVKTRSGKSDPNIVLSKTVMAEV
                     TTNKAAVLSSDATMDARFSGAHSIIMQGIRSTMTLPLLYANELLGIMHLDSLFTSNAF
                     TEKDLQICTGMAQQAAISIQNARLASRIEREAQTRAQISRLIPPSIVEQVVKGELTIE
                     KGGRLSEVTMLFSDIRGFTTMSDGRPAQEIVNTLNEYFEVMVDILFKYSGTLDKFVGD
                     EIIGLFGAPISIEDAPFKAVACGLAMLSALAEFNRTRGSENLPP"
     gene            1488..1569
                     /locus_tag="VMZ53_23295"
     tRNA            1488..1569
                     /locus_tag="VMZ53_23295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1522..1524,aa:Leu,seq:cag)
     gene            complement(1659..3725)
                     /locus_tag="VMZ53_23300"
     CDS             complement(1659..3725)
                     /locus_tag="VMZ53_23300"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31459.1"
                     /translation="MSETELRARIDELEQTVRELRERHAVLTKLDAATQPLVDPRDIM
                     QTTARILAEHLDVDRCAYANVENETVFDITGDWPRGVPSIVGRWDVALFGPACVSHML
                     ENKPYVVDNVMTDPRISPELRASYRATTIEAVICVPLHKQGVFTAAMAVHQKVPRRWT
                     PAEIELVTTVVGRCWEALERAAVTRTRVAAEAALADNRARLEYAVLLSGVGFWYCDLP
                     FDELLWDDRVKAHFWLPPDARVTIDLFYDRIHPDDREATRAAIDTSIANNQRYDVIYR
                     TVDPTTKRVKHVRAIGGAAYGPDGTPTRFDGVTLDVTALREQDQRKDEFLATLAHELR
                     NPLAPIRTGLELLELAQDPADARRTVATMKRQLLHMVRMVDDLLDISRVTLGKVTLVS
                     SRTDLRDIVDSALETTRGLIDAAGHTLEIELPQGPLPLDVDATRISQVVANLLSNAAK
                     YTPRGGRIRIEASREDNGWLDVRVSDTGIGIAADALPAVFEMFTQLGQSIDRAQGGLG
                     IGLTLARRLIEMHNGTITAASAGPNRGSTFTVRLPLALSNAAANDAAPPTTPRRTTPG
                     GLRILVVDDNVDAAEMLAMLLDRDGNEIRVAHSGPDCLVLATELRPHVVLLDIGLPGL
                     DGYEVARRLRADHSLAQPLIVAISGWGTVEDRKKAHEAGFDQHLVKPIDHAALAKLLA
                     TASTSA"
     gene            3883..5463
                     /locus_tag="VMZ53_23305"
     CDS             3883..5463
                     /locus_tag="VMZ53_23305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31460.1"
                     /translation="MRVVVLVLLLTATASADDAWSPIDSRGWPTGVGLDELRELHTCE
                     PGELEPSEGGAIGCRPAFVSEGPRIGMSVGWSSGPAFGSERVAGGTHALGVELDYAIT
                     RHLQLSGLYELAGIAPSRMATGDGKRSQHFFALARMRLFADEVGRDAWTLGAGAGWAL
                     RAASLGDDAPIARVSIARDIGMFLDASNTVGAGIELAYERTLDADALSTVSLSYRMGF
                     ELNVREPQNLGRPAPARSTWRTITGDAYLGNALGLGVSLGLRASSVLSLQTSAGFQFG
                     TSEFAKQHGFDDASWSLLSGPRLSLPFDEVAPYVQVQAGPAWFSTVDGPEVRGLAHAE
                     LGTRIFAGCGGTIDAGVWLRGDIETRDLTSGGLLMRLGTGPVRAHCQPSSSGAFFASR
                     PVDPPPPPPPSPPARTDFSSSGEVVSTGGYAGGSANVYVAQPPVVIVQQPFVLEVELG
                     VAVAGFVVQVDPRMLPLDRMRGVRGVSIELVGSASAVASFEGALRGTLDRNGVRVDAW
                     STVITGSPRVIARITVTP"
     gene            5511..5900
                     /locus_tag="VMZ53_23310"
     CDS             5511..5900
                     /locus_tag="VMZ53_23310"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HUS31461.1"
                     /translation="MRFVWIVIARLLFAAVFIVAGIKHFSGTLINDAAAAGAPAPSVL
                     VPLAGVLAIVGGLSIAVGAFTRIGALVLVAYLVLQTVLTGPELVNFALLGGALAFFYF
                     GSGPISVDDRLAATHRSWRRYIDLQRA"
     gene            complement(5901..6641)
                     /locus_tag="VMZ53_23315"
     CDS             complement(5901..6641)
                     /locus_tag="VMZ53_23315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31462.1"
                     /translation="MTALVAGCGRVAFDPLSDGRAGDDAVTDGSSLTFCETVMPPPTF
                     CADFDSSLDPMVGFSSALLMNGGRVSVSPMAFSGRGSLESAFDDPPTPGQYTTATLIY
                     EAQAPVSSIALDYEVYFPARPTSGSHIESQDIAIATASGTFFYDLDIFPNAPDHFLQE
                     LSAGTFMVNNVNIPQVSTGVWHHVRMTLDLAAATHTFAIDGSTLSSGPTNFPLIPGVP
                     RLEAGINYEINSPTGNKIYVDNLVLYWQ"
     gene            complement(6700..8172)
                     /locus_tag="VMZ53_23320"
     CDS             complement(6700..8172)
                     /locus_tag="VMZ53_23320"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31463.1"
                     /translation="MAIELDDTAAPPPPIGVAATERPAPVVAEGTATAPGKKAVPSTP
                     SDELPAGMRLGHFQLQKKLGAGGMGEVYLATDLALDRPVAIKVLPSGTSSGTARERLI
                     REARAQARVQHPNVGHIYFIGEDQGRLYFAMEYVTGETLGERLAKGPMTAEDALAVIH
                     DAVLGLREAQRSGFTHRDVKPSNLMVDNHGVVKVLDFGIAAGGTSSPDADASGPIAQT
                     TLAGTPLYMAPEQARGEPIDFRADIYALGATLYQLVSGQPPFSADSVEKLITLHASAD
                     RPLIPKGRVPKTQQTALDTLISRMMASKPKDRFASYDDLLRAIENVSTQYTRPAGVWV
                     RAIAFGIDITLLTIILSITLSLIGANDKDVPMGALLMPLLALVDMLLTVRFGSTPGSA
                     LFELQVVDVRTGARPGWRAALLRIAWSYGPLILLDWVALLYKPLHAPDRLQDLTKVAY
                     VAWMVMVAYRLVHASLRVPGKRAPWDRRSNTMVRYRSGRR"
     gene            complement(8186..9400)
                     /locus_tag="VMZ53_23325"
     CDS             complement(8186..9400)
                     /locus_tag="VMZ53_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31464.1"
                     /translation="MWRIAIAVGAILVGACRGNSERAKDRSAAGSGSAVEEAVSKVVA
                     TCAETTGTIEIRRKGQARWEKAEVGTTFRERDWVRTGSRAFARLRFSGGGFLDLQENT
                     SVLVDRAIMVEQGMLVGVADAKQPFIIKAADGSELNVTASGEGAEFRLVATAGKGVEI
                     AVTKGSLELANADGKRVVPAGEGVELSKQRLGDAIKLLPFPRSLSPGIDARFFYTPKM
                     TIPLVWSHVPNATSYYVQVARDAEFHSLVYSGDVTDAKGSFSPDALGMYAWRAAARDA
                     GGHLGEFGYGRRIYCEADQPKDFLVGPRDNFKLDYAKKAPTIEFSWQSQGESKKYKLV
                     IERDAPGADPIVSTTTGTQMLAETLEDGVYYWGVYAIDGDKEQPMFLNLRQLTIHKLE
                     GPKIKTEVHWNK"
     gene            9425..11182
                     /locus_tag="VMZ53_23330"
     CDS             9425..11182
                     /locus_tag="VMZ53_23330"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31465.1"
                     /translation="MTMDSSGLGAQATVRCHACNARGSGKGNCKQCGTPFSKRHPPGT
                     ILGSYRLEEVIGEGGMGIVYLATHTRLGRRVAIKMLRSHYTTNPQAIHRFFAEARAVN
                     KIHHPNIVQITDFIEQPGDDNYYVMELLEGTNLAHLIVHGGMISLARTFAIMTQVASV
                     LEAVHRAGIVHRDLKPENVFLIERGGQQDFVKLVDFGVAKLIDDTDDFVATNNTNPGA
                     ILGTPDYMSPEQASAVPVDHRTDIYAFGAMLYELVTGRLPLKGANFGELVVQLMTVTP
                     TPPSQLSLPHEVPPALEELIMSCLAKQTIDRPDSMETIGERLGEIAEEQGWIVYELNV
                     RPSGPVHARPETNPPPMPGGRKTPPAGVPPLRRPQTTGTPPLKAKTPTPGGSPPLAHA
                     ETAEALPAEAKPDTSPSTVRSPSALLADRLAPTMLAPGPEPVAAPKPTPRRFPMWMLA
                     AGAGVVIAIVVVVVALAGGAKRDEAAAKVDAQLRIADERIAAGKLVAPGGDDALEHLL
                     EAKRLAPANKGVADRLHKLAREYEQLADQAIAAGTFAEAAAHLQVVLSAEPDNKAAAA
                     KMKDVEAHMLERQRPGAPR"
     gene            complement(11179..13056)
                     /locus_tag="VMZ53_23335"
     CDS             complement(11179..13056)
                     /locus_tag="VMZ53_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31466.1"
                     /translation="MRRIVALVALLIARTAVAGEVVSTGPLAMRASGDLVLGRDATAT
                     IHVRGEGDIHLVTSAGTLSELVSEGGARRATLTLPSQQFPQRAIIAALDSAGTVVDWL
                     AVPLAGAARVKIDTEPRATVVVRVGTVEFGPVAADGHGVANVAILVPPGITQATTIAT
                     GKKGAIRDKPMQLGVAPFARTLAICAPMGDHVSVIAITPTGAASAVAPKVTASTGMTD
                     APVAAANGVFISKHHAAPAGDTAEMAASFAGEEAQRSTCTLRVPPEAPSAIKIVGAPS
                     FVAGSGVVTLALSLDYPGQRRAMAVDAITLSADTGTIAAPQRTDDGWTAAWTLPDRFD
                     GRRNAHVTATVDVPGGSPVTATFELELVAAAAERIAVSAPARVRADGAAGGNVEARVL
                     DRFGNVVRVTGLKPRARGRVGSFTGADGTAATYVAPRTRDPGDDVIEVVDPVSGLTGR
                     ATVHLDALPRRFAFALRAGYLSNLGRVGTPVGMASIDARLPILDEKLTLGAQLGGYTT
                     TVIAMDGVESVTGEIKSMPILARAAYRRTLGPLDAWAGAGAGVALTSSEVSSASAGTQ
                     REAANRFAATVFAGAGKRVGPAWLVFEAAYLHATMPSDALLTGRTGGVLATAGFAYEL
                     R"
     gene            complement(13053..>15671)
                     /locus_tag="VMZ53_23340"
     CDS             complement(13053..>15671)
                     /locus_tag="VMZ53_23340"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HUS31467.1"
                     /translation="GMLDPTMAGTGTVAVASSLGPSAVSGAVVILPGAPASVVVSPPS
                     VTLSADAAPLTFTAAVTDADGNTTAPSALTWSVTSGPVTAISSGTGVFDPKRAGMGTI
                     AATTANNISGTAMVTVTPGKAATLAITPPTLVTSQGGAPTTFSVTGGDADGNPTTDIG
                     SLTWSIATGPIASIGAATGVLQPTAAGSGTIRATSSYGPIGTTGTIQILAAGALVPVI
                     AVPSTVSVGQTFTLAMTVSNPGQDAAANVSACTLVVGGTGTASIVTTPAAVPTIAPAG
                     NATLSWTLTATGAGSLTFTTCAQGTDAVTSAPLSTPASSSSTVVSPPQLAATFTVPAI
                     VGRGATFPVSMVVTNSGGATASNVVASALTTTGTASVTLVSSPAAGASIAPAGTTTFQ
                     WTYKATAMGTVQFHGTAAGTDAVSGSPAATPTANSSTVDVVEAYLAASDMFGDGSTFA
                     FVAGYRGKVYAGPNKTGSSAIRVATDATTVEPLTFTFSRDASGNTSANTATPYTSIGA
                     AGCTHNTTGCGPDNEDLRGMFKSFTWGGVEWLLQSGASVALGAAYIYLSTSSTTSLPF
                     SFVDLSSAMSSSHGASAVAVVGSRLYIGTSGDNGGRAQLLALTTSPTAPGLNAGGADL
                     DDMHLERIGRWTTSANPERVDAIGGVNGIAYAMNKNAWVRAENTSPSPYPSLCLPLVC
                     YPDWTDITPSASAYSNKASRSSTKAGGLEPADRAVPQLALFGGRLFVARNTTAGPQLW
                     ACTPSGGKCDSNDWTLVAANSTGDTQLTQFNDTTLTSITMLVATPTLLYVGFDSANGI
                     QIFRTANSAATARSAFEGLAGCSAANHPAACGGYGGAGLGDVTNTRIFDAKAVGSSVW
                     LSVGNGTAPADLVMLP"
BASE COUNT         2534 a         5078 c         5341 g         2718 t
ORIGIN      
        1 cggcggcagg ttctcggacc cgcgggtgcg gttgaactcg gcgagcgccg agagcatcgc
       61 gaggccacat gccaccgcct tgaacggcgc atcctcgatc gagatgggcg cgccaaagag
      121 gccgatgatc tcgtcgccga cgaacttgtc gagcgtgccg ctgtacttga agaggatgtc
      181 caccatcacc tcgaagtact cgttcaaggt gttgacgatt tcctgcgccg gtcgcccgtc
      241 cgacatcgtg gtgaagccac gaatatcgga gaacagcatc gtgacttcgg agaggcggcc
      301 gcccttctcg atcgtgagct cgcccttgac gacctgctcc acgatgctgg gcggaatgag
      361 gcggctgatc tgcgcgcgcg tctgcgcttc gcgctcgatg cggctcgcga gcctcgcgtt
      421 ctgaatcgag atggcagcct gctgcgccat gccggtacag atctgcagat ccttctcggt
      481 aaacgcgttc gacgtaaaca gcgagtcgag gtgcatgatg ccgagcagct cgttcgcata
      541 gagcagcggc aaggtcatcg tcgaccgaat gccctgcatg atgatcgagt gcgcaccgga
      601 aaaccgcgcg tccatcgtgg catcgctcga gagcacggcc gccttgttcg tcgtgacctc
      661 ggccatgacg gtcttcgaca gcacgatgtt cggatcgctc ttgcccgagc gcgtcttcac
      721 gaaccgcgga gcaagctcgc ccttctcgtc catcagcaga atcacgccgc ggtccgcacc
      781 aacgagctcg aacgccttat cgagaatctt cggcagcagc ttgtcgaggt cgagctcgct
      841 gccaaccgcg cgcgccagct cgtggccgat gcgcaagcgc tcgtagtcgc gccgcagaat
      901 cttgtcgtcg ccaatctggc gctccggcat gaagtcgccg gtgttggcct gaatgcgtcc
      961 acgaatgtgg ctctcggtca gccccggcgc gatggtcacg cgctgaagcg catcgtccgc
     1021 cttcggcttg tcgacaaaga cgatgcgggt cgagcccatc gtgaactcgt cgccatccgc
     1081 caaataatga tcgctgacgc gctccccacg caggaacgtg ccgttcaacg accgcaggtc
     1141 acgcagcaga aaccgcccat ccgcggagcg ctggatctgc gcatgttcct tcgagatgat
     1201 gcgatccaag atctggatcg tattgtcggg atgacgaccg atcgtattga aggcggcgag
     1261 ctcgaactcc tgccgctctt ccccggaaat gacgatcagc tttgccatcg cgagctaacc
     1321 agaaaccctt ctcttcgatg ctacgtaagc ggcccttcct agtaaagccg gcccgacgac
     1381 caactccgtg cgcctacacg ggaaataggg ggttcaccgg ccgattaggg cgtggtttcg
     1441 gtcttgtgct cgtccaagag atgtggcatc agttcgcccg cacgtacgcg atcgtggcgg
     1501 aattggtaga cgcgctggat tcaggttcca gtgggtaaca ctgtgggggt tcgagtcccc
     1561 ccgatcgcac gaaggccttt cgttccaacg agcgagaggc ctttcgattt ttcgggaccg
     1621 cgaatcggga gatgggcgcc ggcactttca gagcggtatc aggcgctcgt cgaggccgtt
     1681 gcgagcagct ttgcgagcgc ggcgtgatcg attggcttca cgaggtgctg gtcgaagcct
     1741 gcttcgtgcg ccttcttgcg atcttccacc gtgccccagc cggagatggc cacgatcaac
     1801 ggttgggcga gcgagtgatc ggcgcggagg cggcgcgcga cctcgtagcc gtcgaggcct
     1861 ggcaggccga tgtcgaggag gacgacgtgc gggcgcagct cggtggcgag gacgaggcaa
     1921 tcgggaccgc tgtgtgcgac gcgaatctcg ttgccgtcgc ggtcgagcag catcgcgagc
     1981 atctctgctg catcgacgtt gtcgtcgacg acgaggatcc gcaggccgcc gggtgtggtg
     2041 cgtcgcggcg tggtgggcgg agcggcgtcg ttggcggcag cgttcgagag tgcgagtgga
     2101 aggcggacgg tgaacgtgct gccgcggttt gggccggcgc tcgcggcggt gatcgtcccg
     2161 ttgtgcatct cgatcaggcg acgtgcgagc gtgaggccga tgccgaggcc gccttgcgcg
     2221 cgatcgatcg attggccgag ctgcgtgaac atctcgaaca cggcgggaag ggcgtctgct
     2281 gcgatgccga tgccagtgtc cgagacccgg acgtcgagcc acccgttgtc ttcgcggctc
     2341 gcttcgatgc ggatgcggcc gccgcggggc gtgtacttcg ccgcgttgga gaggaggttc
     2401 gcgacgactt gcgagatgcg cgttgcatcg acgtcgaggg gcagcgggcc ttgtgggagc
     2461 tcgatctcga gcgtgtggcc ggcggcgtcg atcaggcccc gcgtggtctc gagcgcgctg
     2521 tcgacgatgt cgcgcagatc cgtgcggctg gagacgagcg tgaccttgcc gagcgtgacg
     2581 cgcgaaatgt cgaggaggtc gtcgaccatg cgcaccatgt gaagcagctg gcgcttcatt
     2641 gtcgcgacgg tgcgccgtgc gtccgccggg tcttgcgcca gttcgaggag ctcgaggccg
     2701 gtgcgaatcg gtgcgagtgg gttgcggagc tcgtgggcta gggtcgcgag gaattcatcc
     2761 ttgcgttgat cctgctcgcg aagcgcggtg acatcgaggg tgacgccgtc gaagcgcgtc
     2821 ggagtgccgt cggggccgta ggctgcgcca ccgattgcgc ggacgtgctt cacgcgtttg
     2881 gtcgtcgggt cgacggttcg atagatgacg tcgtagcgct ggttgttggc gatcgacgtg
     2941 tcgatggcgg cgcgcgtggc ttcgcggtcg tcgggatgga tgcggtcgta gaacagatcg
     3001 attgtcacgc gggcgtcggg cggcaaccag aagtgcgctt tgacgcgatc gtcccagagg
     3061 agctcgtcga acggcagatc gcagtaccag aagccgacgc ccgagaggag caccgcgtac
     3121 tcgagacgcg cgcgattgtc ggcgagtgca gcttcggcag cgacgcgtgt gcgcgtgacc
     3181 gcggctcgct cgagcgcttc ccagcagcgg ccgacgacgg tcgtcacgag ctcgatctcg
     3241 gcgggcgtcc agcggcgcgg caccttctgg tggacggcca tcgctgcggt gaagacgccc
     3301 tgcttgtgaa gcggcacgca gatgaccgct tcgatcgtgg tcgcgcgata cgaggcgcgg
     3361 agctcgggcg aaattcgtgg gtccgtcatc acgttgtcga cgacgtacgg cttgttctcg
     3421 agcatgtgag agacgcacgc cggaccaaac agtgcgacat cccaacgccc gacgatgctc
     3481 ggcacgccac gcggccaatc ccccgtgatg tcgaagacgg tctcgttctc gacattcgcg
     3541 tacgcacacc gatcgacgtc gagatgctct gcaaggatgc gcgccgtcgt ctgcatgatg
     3601 tcgcgcggat caaccagcgg ctgcgttgcc gcgtcgagct tggtcagcac cgcatgccgc
     3661 tcgcggagct cgcgtacggt ttgctcgagc tcgtcgatac gcgcacgtag ctctgtctcg
     3721 gacacgtgcg gcgaggatag ccagagttcg ggcaatgacc aagccagcga gcagtgcgcg
     3781 agcggcacac ccggcgtgat cgttcattca cgtggtgcag ccgctaaccg cttgtgatcg
     3841 cgttggaacc gggcggtatg ctgattgctg agagcgctcc cgatgcgcgt cgttgtcctc
     3901 gtcctgctgc tcactgcgac cgcctccgcg gacgacgcct ggtcgcctat cgactcgcgc
     3961 ggctggccta cgggcgtggg cctcgacgag ctgcgcgagc tgcatacgtg cgaaccgggt
     4021 gagctcgagc cttccgaagg cggcgcgatc ggctgccggc ccgcgtttgt gagcgaaggc
     4081 ccgcggatcg ggatgagcgt cggctggtcg tcgggtcccg cattcggcag cgagcgcgtc
     4141 gccggcggta cgcacgcact cggcgtcgag ctcgactacg cgatcacgcg acacctgcag
     4201 ctgtccggac tctacgagct cgccggcatc gcgccttcgc gcatggctac gggcgacgga
     4261 aagcggagcc agcacttctt cgctctcgcg cggatgcggc tctttgcgga tgaggtcggc
     4321 cgcgatgcat ggacgctcgg cgctggcgcg ggctgggctc ttcgcgcggc gagcttgggc
     4381 gacgatgcac cgatcgctcg cgtgtcgatc gcgcgcgaca tcggcatgtt cctcgacgcg
     4441 tcgaacaccg tcggtgcagg catcgagctc gcgtacgagc gcacgctgga tgcggacgcg
     4501 ctgtcgacgg tgtcgctcag ctatcgcatg ggcttcgagc tcaacgttcg cgagccgcaa
     4561 aatctcgggc ggcctgcccc cgcgcgaagc acgtggcgga cgatcaccgg cgacgcgtat
     4621 ctcggaaatg cgctcgggct tggcgtgtca ctcggccttc gcgcgagcag cgtgctgagc
     4681 ctgcagacca gtgccggctt tcaattcggc acgagcgagt ttgcgaagca gcacggcttc
     4741 gacgacgcgt cgtggtcgct gctatcggga ccgcgcctct cgctgccatt cgacgaggtc
     4801 gcgccatacg tgcaggtgca agcagggccc gcgtggttct cgacggtgga tggccccgag
     4861 gttcgcggcc tcgcgcatgc ggagctcggc acgcgcatct ttgctggatg cggcggcacg
     4921 atcgatgcgg gcgtctggct ccgcggcgat atcgagacgc gcgacctgac atcgggcggc
     4981 ttgctgatgc gcctcggaac gggccccgtg cgcgcacact gccaaccatc gtcgagcggc
     5041 gcgttcttcg cgtcgcggcc cgtcgatccg ccaccgcctc cgccgccatc accgcccgcg
     5101 cgtactgatt tctcgtcgag cggcgaggtc gtcagcaccg gcggttacgc gggcggctct
     5161 gccaacgtct acgttgcgca gccaccggta gtcatcgtgc agcagccgtt cgtcctcgag
     5221 gtcgagctcg gcgtcgcagt cgcgggcttc gtcgtgcagg tcgatccacg gatgctcccg
     5281 ctggatcgaa tgcgcggcgt tcgcggcgtg tcgatcgagc tcgtcggctc cgcgtcggcg
     5341 gttgccagct tcgagggtgc tctgcgaggc acgctggatc gcaatggcgt gcgcgtcgat
     5401 gcgtggtcga ccgtaatcac gggctcaccg cgcgtcattg ctcgcattac ggtgacgccg
     5461 tgacatcggc gcacgcttga cgctgcttgc ggtggcggca ccttttcaat atgcggttcg
     5521 tctggatcgt cattgcaagg ttgctgttcg cggcggtctt tatcgtcgct ggcatcaagc
     5581 attttagcgg cacgctgatc aacgacgccg cagcggcggg cgcgccggcg ccgagcgtgc
     5641 tcgtgccact ggcgggcgtg ctcgcgatcg tcggcgggct ttcgattgcg gtcggcgcgt
     5701 tcacgcgcat cggtgcgctg gtcctcgtcg cgtatctggt gttgcagacg gtgttgaccg
     5761 ggccggagct cgtgaacttc gcgctgctgg gaggcgcact cgcgttcttc tactttggat
     5821 cggggccgat cagcgtcgat gatcgactcg cggcgacgca tcgctcgtgg cggcgctaca
     5881 tcgatctgca gcgcgcgtag tcactgccaa tacagcacga ggttgtcgac gtagatcttg
     5941 tttcccgtcg gcgaattgat ctcgtagttg atgccggctt cgaggcgcgg cacgcctggg
     6001 atgagcggga agttcgttgg acccgagctg agagtgctgc cgtcgatcgc gaacgtgtgc
     6061 gtcgccgccg cgaggtccag cgtcatacgg acgtgatgcc agacgcccgt cgagacctgc
     6121 gggatgttca cgttgttgac catgaaggtg cccgcggaga gctcctgcaa gaagtgatcg
     6181 ggggcgttcg ggaagatgtc gaggtcgtag aagaacgtcc cggacgcagt cgcgatcgcg
     6241 atgtcttggc tctcgatgtg actgccactg gtcggacggg ccgggaagta gacctcgtag
     6301 tcgagcgcga tactcgagac cggggcctgg gcctcgtaga tcagcgtggc tgtcgtgtat
     6361 tggcccggcg tcggcgggtc gtcgaacgcc gactcgagcg agccacgtcc ggagaacgcc
     6421 atcggtgaga ccgagacgcg gccaccgttc atcagcagcg cgctcgagaa gccgaccatc
     6481 ggatcgagag agctgtcgaa gtccgcgcag aacgtgggcg gcggcatcac ggtctcgcag
     6541 aatgtcagcg acgagccgtc ggtcacagcg tcgtcgccag cgcgtccgtc actcagcgga
     6601 tcgaacgcga cgcgaccgca gccggcgaca agagccgtca cacatactcc ccaccgcggc
     6661 actacctcga tggtacgccg agacgcgaga cgttgcagct tagcgacggc ccgagcggta
     6721 gcggaccatc gtgttcgagc gacgatccca cggcgcgcgc ttgcccggga cgcggagcga
     6781 ggcgtgcacg aggcggtacg cgaccatcac catccaagcg acgtacgcga ccttcgtcag
     6841 atcttgtagg cggtccggcg cgtggagcgg tttgtagagg agcgcgaccc aatcgagcag
     6901 gatcagcggg ccgtacgacc acgcgatgcg caggagtgct gcgcgccaac ctgggcgtgc
     6961 acccgtccgg acgtcgacga cttggagctc gaacaacgcg gagcccggcg tgctgccgaa
     7021 cctgaccgtg agcaacatgt cgacgagcgc aagcagaggc atgaggagcg cgcccatcgg
     7081 gacgtctttg tcgttcgcgc cgatgaggct gagcgtgatg ctgaggatga tcgtgagcag
     7141 cgtgatgtcg atgccgaatg cgatcgcgcg cacccagacg ccggcgggac gcgtgtattg
     7201 cgtcgacacg ttttcgatgg cgcgcagcag atcgtcgtag ctcgcgaagc ggtcttttgg
     7261 cttcgacgcc atcattcgcg agatgagtgt gtcgagcgcg gtctgctgcg tcttggggac
     7321 gcggcctttg ggaatcaggg ggcgatcggc gcttgcgtgg agcgtgatca gtttctcgac
     7381 ggagtccgcc gagaacggcg gttgtccgga gacgagctga tagagcgtgg caccgagcgc
     7441 gtagatgtcg gcgcggaaat cgatcggttc gccgcgcgct tgttcgggcg ccatgtagag
     7501 cggcgtgccg gcgagcgtgg tttgcgcgat cgggccactc gcatcggcgt ccggcgatga
     7561 cgtgccaccg gctgcgattc cgaagtcgag cactttgacg acgccgtgat tgtcgaccat
     7621 caagttggac ggcttgacgt cgcgatgcgt gaagcccgag cgctgtgctt cgcggaggcc
     7681 gaggacggca tcgtggatca cggcgagtgc gtcttcggcg gtcatcgggc ctttggcgag
     7741 gcgctcgccg agcgtttcgc ccgtgacgta ctccatcgcg aagtagaggc ggccttgatc
     7801 ttcgccgatg aagtagatgt gcccgacgtt tggatgttgg acgcgagctt gcgctcgagc
     7861 ttcgcggatc aggcgctcgc gtgccgtgcc cgaggatgtt ccgctcggca gcactttgat
     7921 cgcgactggc cgatcgagcg cgaggtccgt cgcgaggtag acctcgccca ttccgcctgc
     7981 gccgagcttc ttctggagct ggaagtggcc gagccgcatg ccggcgggca gctcgtcgga
     8041 tggcgtcgac ggtacggcct tcttgcctgg agcggtcgcc gtgccttcag cgacaaccgg
     8101 cgctggacgt tccgtcgctg ctacgccgat cggcggaggc ggcgctgcgg tgtcgtcgag
     8161 ctcgatcgcc acgcctcatc atacgtcact tgttccagtg aacctcggtc ttgatcttcg
     8221 ggccttcgag cttgtggatc gtcagctgcc ggaggttgag gaacatcggc tgttctttgt
     8281 ctccgtcgat cgcgtagacg ccccagtagt agacgccgtc ctcgagcgtc tcggccagca
     8341 tctgcgtgcc cgttgtcgtg ctgacgatcg gatcggcgcc gggtgcgtcg cgttcgatca
     8401 cgagcttgta cttcttcgac tcgccttgcg actgccacga gaactcgatc gtcggtgcct
     8461 tcttcgcgta gtcgagcttg aagttgtcgc gcgggccgac gaggaagtct ttcggttggt
     8521 ccgcttcgca gtagatgcgg cggccgtagc cgaactcgcc gaggtggcct ccagcatcgc
     8581 gagcggcagc acgccacgcg tacatgccga gtgcgtccgg cgagaacgac cctttggcgt
     8641 ctgtcacgtc gcccgaatac acgagcgagt gaaactccgc gtcgcgcgcg acctgcacgt
     8701 agtagctcgt ggcgttcggc acgtgactcc acacgagcgg gatcgtcatc ttcggcgtgt
     8761 agaagaaccg cgcgtcgatg ccgggcgaga ggctgcgcgg gaatggcagc agcttgatcg
     8821 cgtccccgag cctctgcttc gagagctcga cgccttcgcc ggcggggacc acacgtttgc
     8881 cgtctgcatt tgcgagctcg agcgagcctt tggtgaccgc gatctcgacg cccttgcccg
     8941 ccgtcgcgac gagccggaac tcggcgccct cgccgctcgc cgtgacattc agctcggagc
     9001 cgtcggcggc tttgatgatg aacggctgct tcgcgtcggc gacgccgacg agcatgccct
     9061 gctccaccat gatcgcgcgg tcgacgagga cggacgtgtt ttcctgcagg tcgaggaagc
     9121 cgccgccgga gaaccgcagc cgcgcaaacg cccgagagcc tgtcctcacc cagtcgcgct
     9181 cgcgaaatgt agtgccgact tctgcctttt cccagcgggc ctggcccttg cgtcggatct
     9241 cgatcgtgcc ggtcgtctcg gcgcacgtcg cgacgacctt cgagacggct tcctcgacgg
     9301 cagagcccga gccggccgca gaacggtctt ttgcacgctc gctgttaccg cggcatgccc
     9361 cgacgaggat cgcccccaca gcgatagcga ttcgccacac ccaagtggca tcatagcatg
     9421 tgggatgacg atggactcga gtgggctggg ggcccaggcg acagtgcgct gtcatgcctg
     9481 caatgcgcgt gggtccggca agggcaactg caagcagtgc ggcacgccct tcagcaagcg
     9541 gcacccgccc ggcaccatcc tcggctcgta tcggctcgaa gaagtaatcg gcgaaggcgg
     9601 gatgggcatc gtctatctcg ccacccacac gcggctcggc cggcgcgtcg cgatcaagat
     9661 gcttcgctcg cactacacga cgaacccgca agcgattcat cgcttcttcg ccgaggcgcg
     9721 agcggtcaac aagatccacc atccgaacat cgtccagatc acggacttca tcgagcagcc
     9781 cggcgacgac aactactacg tgatggagct gctcgaggga acgaacctcg cacatctcat
     9841 cgttcacggc ggcatgattt cgctcgcgcg aacgtttgcg atcatgacgc aggtcgcatc
     9901 ggtcctcgag gccgtgcatc gcgcggggat cgtgcatcgc gatctgaagc cggagaacgt
     9961 gttcctgatc gagcgcggtg gtcagcagga cttcgtcaag ctcgtcgact tcggtgtcgc
    10021 gaagctgatc gacgacaccg acgacttcgt cgcaacgaac aacacgaatc caggcgcgat
    10081 cctcggcacg cccgattaca tgtcccccga acaagcgagc gccgtcccgg tcgatcaccg
    10141 cacggatatc tacgcgttcg gcgcaatgtt gtacgagctc gtgacgggtc gccttccgct
    10201 gaagggcgcg aacttcggag agctcgtcgt gcagctcatg acggtgacgc cgacgccgcc
    10261 gagccagctg tcgctgccgc acgaggtccc gccggcgctc gaggagctca tcatgagctg
    10321 cctcgcgaag cagacgatcg atcgccccga ctcgatggag acgatcggcg agaggctcgg
    10381 cgagatcgcc gaagagcaag gatggatcgt ctacgagctc aacgtgcgcc cgtcgggacc
    10441 agtgcatgcg cgccccgaga cgaacccgcc cccgatgccg gggggacgca agacgccgcc
    10501 cgcaggtgtt ccgccgctcc gccgaccaca gacgaccgga acgccgccgc tgaaagcgaa
    10561 gacgccgacc cctggtggtt ctccgccgct cgcgcacgcc gagactgctg aagcacttcc
    10621 cgcggaggcg aagcccgaca cgtcgccgag caccgtgcgt tcgccgagcg cgttgctcgc
    10681 ggatcgactt gccccaacga tgctggcgcc cggtcccgag ccggttgctg cgccgaagcc
    10741 aacgccgcgg cgttttccga tgtggatgct ggctgccggc gctggcgtgg tgatcgcgat
    10801 tgtcgtggtc gtggtcgcac tggccggcgg ggcgaaacgc gacgaggcag ctgccaaggt
    10861 cgacgcgcag ctgcgcatcg ccgacgaacg catcgcggcg ggaaagctgg tcgcgcccgg
    10921 tggcgatgac gcgctcgagc atctgctaga ggcgaagcga ctcgcgccgg cgaacaaagg
    10981 ggtcgccgat cggttgcaca agctcgcacg cgagtacgag cagctcgcgg atcaagcgat
    11041 cgctgcgggc acgttcgcgg aggcagcggc gcatctgcag gtcgtgctca gcgccgagcc
    11101 cgacaacaag gcagccgcag cgaagatgaa agacgtcgag gcgcacatgc tcgagcgtca
    11161 gcggcccggc gcacctcgct agcgcagctc gtacgcgaag ccagccgtcg cgagaacccc
    11221 acccgtgcgg ccggtgagca gcgcatcgct cggcatcgtc gcgtgcaggt acgccgcctc
    11281 gaacacgagc cacgctgggc cgacgcgttt accagcgcca gcgaagaccg tcgccgcgaa
    11341 acgattcgct gcttcgcgct gcgtgcctgc gctcgcggac gagacttccg acgaggtcag
    11401 cgcaacacct gcaccggcgc cggcccacgc gtcgagcggg cccagggtgc ggcgatacgc
    11461 ggcgcgcgcg aggatcggca tcgacttgat ctcgccagtg acggactcga ctccgtccat
    11521 cgcgatcacc gtcgtcgtgt agccgccaag ctgtgcaccg agcgtcaatt tctcgtcgag
    11581 gatcgggagg cgcgcatcga tcgaagccat gccgaccggc gttccgacgc ggcctaggtt
    11641 cgacaggtag cccgcacgca gcgcaaacgc gaagcgccgc ggcaatgcat cgaggtgaac
    11701 ggtcgctcgg ccggtgagcc cgctgactgg atcgacgacc tcgatgacgt cgtcacccgg
    11761 atcacgcgtg cgaggtgcaa cgtacgttgc tgccgtgccg tcggcgccgg tgaacgagcc
    11821 aacgcggcca cgagcacgcg gcttcagccc ggtgacgcga acgacattgc cgaagcgatc
    11881 gaggacacgt gcctcgacat tgccaccggc agcgccatct gcacgaacgc gcgccggcgc
    11941 cgagactgca atccgctcgg ctgctgcagc gacgagctcg agctcgaacg tagcggtgac
    12001 cggcgatcca ccgggaacat ccaccgtcgc ggtgacgtgc gcgttgcgcc gaccgtcgaa
    12061 gcgatcgggc agcgtccacg ccgcggtcca gccatcgtcg gtgcgttgcg gcgctgcgat
    12121 cgtgccggtg tcggcgctga gcgtgatcgc atcgaccgcc atcgcacgac gctggcctgg
    12181 atagtcgagc gacaacgcga gagtgacgac gccgctacca gcgacgaagc tcggtgcccc
    12241 cacgatcttg atcgccgacg gcgcctctgg cggaacacgc agcgtgcagg tcgagcgttg
    12301 cgcctcctct cccgcgaacg aagcggccat ctccgcggtg tctcccgccg gtgccgcgtg
    12361 gtgcttcgag ataaagacgc cgttcgcagc ggcgaccggt gcatccgtca ttcccgtcga
    12421 tgcggtgacc ttcggtgcga cggccgacgc ggcgcctgtc ggcgtgatcg caatgaccga
    12481 gacgtggtcg cccatcggtg cgcagatcgc gagcgtgcga gcaaacggtg cgacgccgag
    12541 ctgcatcggt ttgtcgcgga tcgcgccctt cttgcccgtc gcgatcgtcg tcgcctgcgt
    12601 gatgcccggc ggcacgagaa tcgcgacatt tgcgacgcca tgaccgtcag ccgcgacagg
    12661 gccgaactcg acggtgccga cgcggacgac aacggtagcg cggggctcgg tgtcgatctt
    12721 cacgcgtgcg gcgcccgcga gaggaaccgc cagccaatcg acgacggtgc ctgcgctgtc
    12781 gagcgccgcg atgatcgctc gttgcgggaa ctgctggctc ggcaacgtga gcgtcgcgcg
    12841 tcgcgcgccg ccttcgctga cgagctccga gagggtgccc gcgctcgtca cgagatggat
    12901 gtcgccttcg ccgcgcacgt gaatggtcgc agtcgcgtct cgcccaagca cgagatcgcc
    12961 cgaggcgcgc attgcgagtg gccccgtcga gacgacctcg cccgcgacgg ccgtgcgtgc
    13021 gatgagcaac gcaacgagag cgacgatcct cctcatggca gcatcaccaa atccgccggc
    13081 gccgtaccgt tgccgacgct gagccagacc gaggagccaa ccgccttcgc gtcgaagatg
    13141 cgcgtattcg tgacgtcgcc tagccccgct ccaccgtagc cgccgcacgc agcggggtgg
    13201 ttcgccgccg agcatcctgc gagtccttcg aatgcggacc gcgcggtcgc agcggagttt
    13261 gcggtgcgga agatctggat gccgttcgct gaatcgaaac cgacgtagag gagcgtcggc
    13321 gtcgcgacga gcatcgtgat cgatgtcagc gtcgtatcgt tgaactgcgt cagctgtgta
    13381 tcgcccgtgc tgttcgcagc gaccagcgtc cagtcgttcg agtcgcactt gccgcccgac
    13441 ggagtgcacg cccacagctg cggtcccgcg gtcgtgttgc gcgcgacgaa caagcgcccc
    13501 ccaaagagcg cgagctgcgg cacagcgcga tcggccggct cgaggccgcc ggctttcgtc
    13561 gacgagcgcg acgccttgtt gctatacgcc gatgcggacg gcgtgatgtc cgtccaatcg
    13621 gggtagcaga ccagcggcag gcagagcgac ggatacgggc tcggcgatgt gttctcggcg
    13681 cgaacccacg cattcttgtt catcgcgtat gcgatgccat tgacgccgcc aattgcatcg
    13741 acgcgctcgg gattcgccga cgtcgtccac cggccgattc tctcgaggtg catgtcgtcg
    13801 agatcggcgc cccctgcgtt gaggcccggc gccgtcggag atgtcgtcaa cgcgaggagc
    13861 tgcgcgcgcc cgccgttgtc tcccgacgtg ccgatgtaga gccggctgcc aacgacagcc
    13921 acggccgacg cgccatggct cgatgacatc gcactgctca gatcaacgaa gctgaacggc
    13981 agcgacgtcg tggagctcgt cgacaggtag atatacgcgg cgccgagcgc gacggaggca
    14041 ccgctctgca agagccactc gacgccgccc caggtgaacg acttgaacat gccgcgcagg
    14101 tcctcgttat cgggcccgca tccggtcgtg ttgtgtgtgc agccggccgc gccgatcgac
    14161 gtatagggcg tcgcggtgtt cgcgctcgtg ttccccgacg catcccgcga gaacgtaaac
    14221 gtgagcggct cgacggtcgt cgcatcggtc gccacgcgaa ttgccgacga gcccgtcttg
    14281 ttcggcccgg cgtagacctt gccgcgatag cccgcgacga acgcgaacgt gctgccatcg
    14341 ccgaacatat cgctggcggc gagatacgcc tcgacgacat caaccgtgct cgaattcgcc
    14401 gttggcgtag ccgccgggct tccggacacg gcatcggttc cggcggcggt gccgtgaaac
    14461 tggaccgtgc ccatcgcggt cgccttgtac gtccactgaa acgtcgtcgt accggccgga
    14521 gcgatcgacg cgcccgccgc cggcgacgaa acgagcgtta cgcttgccgt gccggtcgtc
    14581 gtcagcgcgc tcgccacaac attcgatgca gtcgcgccgc cgctgttcgt cacgaccatc
    14641 gagaccggga acgtcgcacc acgacccacg atcgccggca ccgtgaacgt cgcggcgagc
    14701 tgcggagggg agacgaccgt cgaggacgag cttgccggcg tcgagagcgg tgcgctcgta
    14761 accgcgtcgg tgccctgcgc acaggtcgta aacgtcagcg agcccgcacc cgttgcggtg
    14821 agcgtccacg agagcgtcgc gttaccagcg ggcgcgatcg tgggcacggc tgccggcgtc
    14881 gtcacgatcg acgcagtgcc cgtcccgccg acgacgagtg tgcacgcgct gacgttcgct
    14941 gccgcgtcct ggccaggatt cgagacggtc atcgccagcg tgaacgtctg tccgaccgag
    15001 accgtcgacg gcaccgcgat caccggaacg agcgcccctg ccgccaggat ctggatcgtg
    15061 cccgtcgtcc cgatcggacc atacgagctc gtcgcccgga tcgtgcccga gccggctgcg
    15121 gttggctgca gcacacccgt cgctgcgccg atcgatgcga tcggtccggt cgcgatcgac
    15181 catgtcagcg acccgatatc cgtcgtcgga ttaccgtccg cgtcgccgcc cgtgacgctg
    15241 aacgtcgtcg gggctccacc ttgtgatgtc accagcgtgg gcggtgtgat cgccagcgtc
    15301 gcggccttgc cgggtgtgac cgtcaccatc gccgtgccgc tgatgttgtt cgccgtcgtc
    15361 gccgcgatcg tccccatgcc agcgcgcttc ggatcgaaga ctccagtgcc gctcgagatc
    15421 gcggtaaccg gtccgctcgt cacggaccac gtgagcgcgc taggcgcggt ggtattgccg
    15481 tcggcatcgg tgactgccgc ggtgaacgtc agcggtgcag catcggcgct cagcgtcacc
    15541 gacggtggtg acacgaccac gctcgccgga gcgcccggca ggatcacgac ggcgcccgac
    15601 accgcgctcg gtcccagcga gctggctacc gcaacggtgc ctgtgccggc catcgtcgga
    15661 tccagcatgc c
//
LOCUS       DATLBZ010000462         5339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1325237, whole genome shotgun sequence.
ACCESSION   DATLBZ010000462 DATLBZ010000000
VERSION     DATLBZ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325237"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..487
                     /locus_tag="VMZ53_23345"
     CDS             32..487
                     /locus_tag="VMZ53_23345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS31468.1"
                     /translation="MARFEGVSPADALDVVQDAFYTLLQRPDQLELREKPEDAARVLS
                     AIVRNAARNLRRRHHNARPHDDVEDAALVSGGPAPDQALEQAVTAAQLEGCMAKLGEV
                     HRHVVTLRVLEELSGDEAAAALGVTPGHVAVLLHRARKQLERCMTESAA"
     gene            complement(526..846)
                     /locus_tag="VMZ53_23350"
     CDS             complement(526..846)
                     /locus_tag="VMZ53_23350"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HUS31469.1"
                     /translation="MNRLVDTSSTMVTLYDFTAKWCGPCRTMKPVLDALAKEYAGKVQ
                     LVEVDVDDDQLTTQQFNVRAMPTYVLVRDGREVGRFVGSRPRAFVAGVLDRALSGDVQ
                     ITAP"
     gene            827..1648
                     /locus_tag="VMZ53_23355"
     CDS             827..1648
                     /locus_tag="VMZ53_23355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS31470.1"
                     /translation="MSTSRFITRDARREDYAAFVGFWGELGLDQAAPDVEWWLEHLAP
                     RTRFLAEPESGKLVAYSLTFAFGERGDVRQIAVDPSVRRQGVGRALFAAIAERFRGEG
                     VRDWRLEVKQDNVAGIALYRAVGMDVIRELLSLRMTAEQVRAFAATRLGGSRVDTVEP
                     ADDAALEARWDLGRGQIARWRKARGPNARLMRIADRALAQFTPDFSAKWGLVFPLYAI
                     DRGHVAAIFGAMTQMELPPIVEMQIPDGVAAKALERAGAKVLERLYEMGGVLDTQ"
     gene            1671..3122
                     /locus_tag="VMZ53_23360"
     CDS             1671..3122
                     /locus_tag="VMZ53_23360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31471.1"
                     /translation="MRLWGTLVVLLLSTRAFAQPADPAPAADPQAPQQAPQPAPQPDA
                     VPAEPPEPIETPTATEAPTSGLRVHILSTGNTYLPMTFDVFSVETKQVVASGAGAVES
                     RGEEAPILELKAGNYKIVRSGESFDARVDYAVATVVADTVVDYVIVVDPDDFAFRGSG
                     PLVGELPKGVEIAGVRLSLNGGGTVLFTQVRNGVGTTSGTTAQLGLFGNFGLVIEKGV
                     HFIDVNADAKLDLFDPVTGSLVPMHDRFQASGLYAYHLENSFLGPYVRMGMVTHVFPG
                     YVYLERDQPGTIIVEHVDGTETSRSFGQKADPDDLRIQVAKPFAPLKLQEELGANIKA
                     VDIDLLVVKVNVGTRVGFGFRQGIMNGLIVARSHGDADPIIRFREVDDYTTLGPVIGA
                     NASVTFARWLFGSAQFGLLAPLTSTDSSQSDNFGGRLLMDFSGTAGFKVPILTNLVTA
                     SADYSFRLERDAFITARTQFEHALMARANITLF"
     gene            complement(3119..3994)
                     /locus_tag="VMZ53_23365"
     CDS             complement(3119..3994)
                     /locus_tag="VMZ53_23365"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HUS31472.1"
                     /translation="MPADTTFLSQIQLFAQLDEDERNVLAQAMTERKANAGEHLFRIG
                     DPGDSMFIVQTGTVELFVKDTVGQKIVLHNAVPGDFFGELSLLDGGSRTASATVLEET
                     LLYVLDREDLLQLFRKRPDAALDMLAAMGRMTRKANALLRERVSKNVNEQVEEERSNL
                     ILRVADVVANFSGSITFLIIHVGLFAFWLTINMTSAAFDPFPFGLLTMAVSLEAIILS
                     TLLLFSSNRQGARDRIRADIEYEINMKAELEVAHLHEKTDKMYEEMVERFARIEKAMN
                     ISRQPGQQVAAAPKA"
     gene            4084..5094
                     /locus_tag="VMZ53_23370"
     CDS             4084..5094
                     /locus_tag="VMZ53_23370"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HUS31473.1"
                     /translation="MESASASQAQMEAFDPLFVVVELDFKTKQHRKISINDIRTSMEA
                     GKFVWIDVDIAQSEEARKLLTTLNLCNPEIVEDAMSREPATQIARYDDYIHLVLSGCR
                     LIGHKFDLERVDAMMGERYLLTLHKGAPVFLEAVRRSYVSDFVRFAQSPSFLLYELWD
                     HLIDNYLNVHKKFEERVEQVQAQLVGDVDDRVFGEASELGSDLLHLRKVVLPARAVLA
                     DLSTRKSPFVSEATQPFLGNMVGTIERVLQDVLVDRDILAGSLNNYMSLVAHKTNQVM
                     SKLTVVSIIFLPLTFLCGVYGMNFDNLPELHLKHGYAYFWGLVLLLLFGLLYMMRRNK
                     LL"
     gene            complement(5091..>5339)
                     /locus_tag="VMZ53_23375"
     CDS             complement(5091..>5339)
                     /locus_tag="VMZ53_23375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HUS31474.1"
                     /translation="EVSDNGPGIPAELRERVFERFFRVEHAQKSAPPSAGVGIGLYIA
                     RQVIELHGGTIRCDTGPLGGARFTMTIGKLPPGATAEG"
BASE COUNT          889 a         1630 c         1829 g          991 t
ORIGIN      
        1 cgctcgcgac cacgcgggcc gacttgctgg aattgcgcgg ttcgagggcg tgagcccggc
       61 ggatgcgttg gatgtcgtgc aggacgcgtt ctacacgctg ttgcagcggc cggatcagct
      121 ggagctgcgc gagaagccgg aggatgcggc gcgggtgctg tcggcgatcg ttcgcaatgc
      181 ggcgcggaat ctacggcggc ggcatcacaa tgcgcggccg catgacgatg tcgaggatgc
      241 ggcgcttgtg agcggtgggc ctgcgcctga tcaggcgctg gagcaggcgg tgacggccgc
      301 gcagctcgag ggctgcatgg cgaagctcgg cgaggtgcat cggcatgtcg tgacgctgcg
      361 cgtgctcgaa gagctgtcgg gcgatgaggc ggctgcggcg ttgggggtta cgcctgggca
      421 tgttgccgtg ctgctgcatc gcgcgcgcaa gcagctcgag cgctgcatga ccgagtcggc
      481 tgcttagggc cgcttagagg gctgcggagg gctgcttagg gctggttagg gcgcggtgat
      541 ttgaacgtcg cccgagaggg cgcgatcgag gacgccggcg acgaatgcgc gcgggcgtga
      601 gcccacgaag cggccgactt cgcggccatc gcgcacgagc acgtacgtcg gcatcgcgcg
      661 cacgttgaat tgttgggttg tgagttggtc gtcgtcgacg tcgacctcga cgagctggac
      721 tttgcccgcg tattccttgg cgagtgcgtc gaggacgggt ttcatcgtgc ggcagggtcc
      781 gcaccacttg gcagtgaaat cgtagagtgt gaccatagtg gacgaggtgt cgacgagccg
      841 attcattaca cgcgatgcgc gccgcgagga ttacgcggcg tttgtcgggt tttggggcga
      901 gctcggactc gatcaggcgg cgcctgatgt ggagtggtgg ctggagcatc tcgcgccgcg
      961 gacgcggttc ttggcggagc cggagagcgg aaagctcgtc gcgtattcgc tgacgttcgc
     1021 gttcggcgag cgcggggacg tgcggcagat cgctgtggat ccgagcgtgc gtaggcaagg
     1081 tgtcgggcgc gcgttgttcg cggcgatcgc ggagcgattt cgcggtgagg gcgtgcgcga
     1141 ttggcggctg gaggtgaagc aagacaacgt ggcaggcatc gcgctgtatc gcgcggtcgg
     1201 catggacgtg attcgcgagc tcctgtcgct gcgcatgacg gcggagcaag tgcgcgcgtt
     1261 tgccgcgacg cgactcggcg gttcgcgcgt cgacaccgtc gagccggcgg atgatgcggc
     1321 gctcgaggcg cggtgggatt tgggtcgggg gcagatcgcg cggtggcgca aggcgcgtgg
     1381 accgaacgcg aggctcatgc gaatcgcgga tcgcgcgctc gcgcaattca cgccggactt
     1441 ctcggcgaag tgggggctcg tgtttccgct ttatgcgatc gaccgcggcc acgtcgcggc
     1501 gatcttcggc gcgatgacgc agatggagct cccgccgatc gtcgagatgc agataccgga
     1561 cggcgtcgcc gcgaaggcct tggagcgtgc gggcgcgaag gtcttggaac gcctctacga
     1621 gatgggtggc gtgctcgaca cgcagtgatg cgtgacgtta cgatcccgga atgaggctgt
     1681 ggggaaccct cgtcgtactg ctgttgtcga ctcgcgcgtt cgctcaaccg gcggaccctg
     1741 cgccggcagc ggatccgcaa gcgccgcaac aagcgccgca accggcgccg cagcccgatg
     1801 ccgttccggc cgagccgccc gagccgatcg agacgccgac ggcgaccgaa gcgccgacgt
     1861 cgggcctgcg cgtccacatc ctgtcgacgg gcaacacgta tctgccgatg acgttcgacg
     1921 tgttctctgt cgagacgaag caagtcgtcg cgtccggcgc gggagccgtc gagtcgcgcg
     1981 gcgaagaagc gccgatcctc gagctgaagg ccggcaacta caagatcgtc cgcagcggtg
     2041 agtcattcga cgcgcgcgtc gattacgccg tggcgacggt cgtggcggac accgtcgtcg
     2101 actacgtgat cgtcgtcgat ccagatgact tcgcattccg cggctcgggc ccgctcgtcg
     2161 gcgagctgcc gaagggcgtc gagatcgcag gcgttcggct ctcgctcaac ggcggcggca
     2221 cggtgctgtt cacgcaggtc cgcaacggcg tcggcacgac gagtggcacg acggcgcagc
     2281 tcggtttgtt cggcaacttc ggcctcgtga tcgagaaggg cgtgcatttc atcgacgtca
     2341 atgccgatgc aaagctcgac ctgttcgacc ccgtcacggg ctcgctcgtg ccgatgcacg
     2401 atcgctttca ggcgagcggc ctctacgcgt atcacctcga gaattcgttc ctcggcccgt
     2461 acgtgcgcat gggcatggtc acgcacgtgt tccccggcta cgtctatctc gagcgcgatc
     2521 agccgggcac gatcatcgtc gagcacgtcg acgggaccga gacgtcgcgc agcttcgggc
     2581 agaaggcaga ccccgatgat ctccgcattc aagttgcgaa gccgttcgca ccgctgaagt
     2641 tgcaggaaga gctcggcgcc aacatcaagg ccgtcgacat cgatctcctc gtcgtgaagg
     2701 tcaacgtcgg aacccgagtc ggattcggat ttcgccaggg catcatgaac ggcctcatcg
     2761 tggcgcgtag ccatggggat gctgacccga tcattcgctt ccgcgaggtc gacgattaca
     2821 cgacgctggg ccctgtcatc ggtgcaaatg cgagcgtgac gttcgcgcgc tggctctttg
     2881 gctccgcgca gttcggtttg ctcgcgccgc tcacgagcac ggatagctcg cagtccgaca
     2941 acttcggcgg ccggctgctc atggacttct cggggacggc gggcttcaag gtgcccatcc
     3001 tgacgaacct cgtcacggcg tcggcggatt actcgttccg cctcgagcgc gatgcgttca
     3061 tcactgcacg gacgcagttc gagcacgcac tgatggcgcg cgcgaacatc acgctgttct
     3121 aggccttcgg cgccgcagcg acctgctgcc ccggctgacg cgagatgttc atcgccttct
     3181 cgatgcgggc gaagcgctcg accatttcct cgtacatctt gtccgtcttc tcgtggaggt
     3241 gagcgacttc gagctcggcc ttcatgttga tctcgtattc gatgtcggca cggatgcgat
     3301 cgcgtgcgcc ttgccggttc gagctgaaga gcagaagcgt cgagagaatg atcgcctcga
     3361 gcgagacggc catcgtgagc aggccgaacg ggaacggatc gaacgccgcc gacgtcatgt
     3421 tgatcgtcaa ccagaatgcg aacaggccga cgtggatgat gaggaacgtg atgctgccgg
     3481 agaagttcgc gacgacatcg gcgacgcgca ggatgaggtt actgcgctcc tcttcgactt
     3541 gctcgttgac gttcttcgac acgcgctcgc ggaggagggc attcgcctta cgggtcatcc
     3601 ggcccatcgc ggcgagcatg tcgagcgcgg cgtctggacg cttgcggaac agctgcagca
     3661 gatcctcgcg gtcgagcacg tagagcagcg tctcctcgag caccgtggcg ctggccgtgc
     3721 gcgatccgcc gtcgagcagt gagagctcgc cgaagaaatc gccgggcact gcgttgtgca
     3781 gcacgatctt ctggcccacc gtgtctttca cgaacagctc gaccgtgccc gtctggacga
     3841 tgaacatcga gtcgcccgga tcgccgatgc gaaacaggtg ctcgcctgcg ttcgccttgc
     3901 gctcggtcat cgcttgcgcg agcacgttgc gctcgtcctc gtcgagctgt gcgaacagct
     3961 ggatctgtga aaggaacgtc gtgtccgctg gcatcggtac tccattatcg agaggaccta
     4021 gagatcgttt ggccgcgacc gtccaggcaa tttgcgccgc aacgagcgta atatgcacgg
     4081 gtggtggaat cagcatctgc ttcgcaggcg cagatggagg cgttcgatcc gctgttcgtc
     4141 gtcgtcgagc tcgacttcaa gacgaagcag catcgcaaga tctcgatcaa cgacatccgc
     4201 acctcgatgg aggccggcaa gttcgtctgg atcgacgtcg acatcgcgca gagcgaagaa
     4261 gcgcgcaagc tcctgacgac gctgaacctg tgcaacccag agatcgtcga ggacgcgatg
     4321 tcccgcgagc ccgcgacgca gatcgcccgc tacgacgact acatccatct cgtgctgtcg
     4381 ggctgccggc tcatcggcca caagttcgat ctcgagcgcg tcgacgcgat gatgggcgag
     4441 aggtatttgc tgacgctgca caaaggcgcg cccgtctttc tcgaggcggt gcggcgctcg
     4501 tacgtgagcg acttcgtccg gttcgcgcag agcccgagct ttctgctcta cgagctctgg
     4561 gatcacctca tcgacaacta cctcaacgtg cacaagaagt tcgaggagcg cgtcgagcag
     4621 gtgcaggccc aattggtcgg cgacgtcgat gatcgcgtgt tcggcgaagc atccgagctc
     4681 ggctcggatc tgctgcacct gcgcaaagtc gtgcttcccg cacgcgcggt gctcgccgat
     4741 ctatcgacac gcaagtctcc gttcgtcagc gaagcgacgc agccgttcct cgggaacatg
     4801 gtcggcacga tcgagcgcgt gctccaagac gtgctggtcg atcgcgacat cctcgccggc
     4861 tcgctcaaca actatatgtc cctcgtcgcg cacaagacga accaggtgat gagcaagctg
     4921 accgtcgtca gcatcatctt cctgccgctc acgttcttgt gcggcgtgta cggcatgaac
     4981 ttcgacaacc tgcccgagct gcacctcaag cacggctatg cgtacttctg ggggctcgtg
     5041 ctcctgttgt tgttcggcct gctctacatg atgcggcgca acaagctgct ctagccttca
     5101 gcggtcgcgc ctggcggcag cttgccgatc gtcatggtga agcgcgcgcc gccgagcgga
     5161 ccggtgtcgc agcggatcgt gccgccgtgc agctcgatca cttggcgcgc gatgtagaga
     5221 ccgatgccga cgcctgccga cggcggtgca gacttctgcg cgtgctcgac gcggaagaac
     5281 ctctcgaaca cgcgctctcg cagctcggcc ggaatgccgg ggccgttgtc gctgacctc
//
LOCUS       DATLBZ010000463         4542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10730268, whole genome shotgun sequence.
ACCESSION   DATLBZ010000463 DATLBZ010000000
VERSION     DATLBZ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10730268"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VMZ53_23380"
     CDS             complement(<1..128)
                     /locus_tag="VMZ53_23380"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HUS31475.1"
                     /translation="MSTDKVIISCALTGAVTTKKHCPAIPYTPVEIAEEAKRAYDAG"
     gene            complement(138..776)
                     /locus_tag="VMZ53_23385"
     CDS             complement(138..776)
                     /locus_tag="VMZ53_23385"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HUS31476.1"
                     /translation="MSAASVLGCPDCPPAREARDLFVQNLGFDVVRAALPFVLIAIVV
                     FAVARVVRRYDGDASVSSTVSAGLVLGIGLGGFLDGIVLHQILQWHEMMSSVVPPDGL
                     VEVKYNMIWDGLFHAGVWLTTVLGVLLLVRAARRGRVRSKRIFGGSLLGGWGLFNFIE
                     GLVDHQLLGLHHVHPGSGQAAWDWGFVLVGGLGFMALGFVTTRSDLAGHSAV"
     gene            970..2244
                     /locus_tag="VMZ53_23390"
     CDS             970..2244
                     /locus_tag="VMZ53_23390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31477.1"
                     /translation="MRIAPLAAVLAAFLVACVHPKPGYFSAQKQAPAVKPVAPTARPF
                     VKMPWVELVDQVQTGPIYRVQIAAEITRDVIPTDDLAIVVSVDDHEVGRYPIVGKYKG
                     RPTKIKQEIELPLGDYEIDYVYQGDHFAGMPFRLAEVPVWGGRTALQLRAHQGTRVSL
                     REKKLWVGRWWANDGPAQAWIIEWARDGQVITTTSGTEAVGIPPEARGLVGGAAGAFR
                     AEKIVQNTIWTYGEEYPLPDVVAQQPGAWAARVVHGSSAPVAVVFTVTQLGTLVEASS
                     RKVMHVGWEPSWSKRLDARSLSVTEVDRLTAKLPHTSSTQPFDEPRTDGIEPAVRVSP
                     AAVRALFRSKQLAESWWEFLSLNRSTPATLTAQPAAFVKGSQPANARRDAAASAKQDA
                     ERRTKLRKLRAQIDELIKTQGFAWKPDEHPHS"
     gene            2457..2804
                     /locus_tag="VMZ53_23395"
     CDS             2457..2804
                     /locus_tag="VMZ53_23395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31478.1"
                     /translation="MNIESIDRSAAYVAAKSAVDVVQKITGGWPSEVAEPAKRCAQNL
                     LSAISEALEHGETTASRRRCLRGALLNAIELAAICDIARAHGLVTEQSLQSTGRTLSL
                     LGMSYHATSVANE"
     gene            complement(2852..3700)
                     /locus_tag="VMZ53_23400"
     CDS             complement(2852..3700)
                     /locus_tag="VMZ53_23400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31479.1"
                     /translation="MAVALVLDFDGTMTDAEAEGRPFRDGYLEDLALLTGASLLDVTK
                     IADEVEDELAAAPASHAFLWMGRPVAPATVDPYLRMVPVAHRILDRYKAIPSHEDRGR
                     LLGSVLYKYNYAKTLGHPVFRAGAGDMLRKLGGTPTWIVTNSDTHAVAGKVAALDREA
                     PGVAWLTSRVRGFARKFDVDDAWTGAASELAIPGLDRAVLLRRRAYFEILADVVKEAG
                     STFDELVVVGDIFELDLALPLSLGARVGLVTSSRTPDYERAFVTAHPRAALIDDLSQI
                     PAFAFG"
     gene            complement(3767..>4542)
                     /locus_tag="VMZ53_23405"
     CDS             complement(3767..>4542)
                     /locus_tag="VMZ53_23405"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUS31480.1"
                     /translation="MKLGGERKELTVLFSDIRGFSAFAEGMGPETLASFLGEYLTPMT
                     DLVLESGGTLDKYIGDAVMAIWAAPVDLPDHAARACEVALKMQEALIALNKKWKTEGK
                     PEVAIGIGINTGAMSVGNMGTPARFDYTVLGDQVNLGARLEALTKEYGVDILVGEATA
                     KAAGPGFVFREIDLVRVKGRAGAAPVYELVGRAGVKVDERFTEALATYRRRDFDAARA
                     QFAALSDDATAAVMAKRCADLASRPPPEGWDGVYEQRSK"
BASE COUNT          795 a         1520 c         1503 g          724 t
ORIGIN      
        1 cctgcgtcgt acgcgcgctt cgcctcttcg gcgatctcga cgggcgtgta cggaatcgcc
       61 gggcagtgct tcttcgtcgt cacggcgccg gtcagcgcgc aggagatgat gaccttgtcg
      121 gtggacacga gcggagccta caccgcgctg tggccggcga ggtcgctccg tgttgtgaca
      181 aagccaagtg ccatgaaccc caggccaccg acgagaacaa agccccaatc ccatgccgct
      241 tgcccgctgc cgggatgcac gtggtgcagg ccgagcagct gatgatcgac gagcccttcg
      301 atgaagttga agaggcccca gccgccgagc agcgagccgc cgaatatgcg cttcgaccgc
      361 acgcgtccgc gtcgcgccgc gcgaaccaac agcagcacgc cgagcaccgt cgtgagccac
      421 acgcccgcgt gaaagagccc gtcccagatc atgttgtact tcacctcgac gagcccatcg
      481 ggcggcacga cgctcgacat catctcgtgc cactgcagga tctgatggag cacgatgccg
      541 tcgagaaatc cgccaaggcc gatgccgagc acgaggcccg ccgacacggt tgacgagacg
      601 ctcgcatcgc catcgtaacg gcgcacgact cgcgccaccg cgaacacgac gatcgcgatc
      661 agaacgaagg gcagtgcggc tcgcacgaca tcgaagccga ggttctgaac gaagagatcg
      721 cgagcctccc gggcgggcgg gcaatcggga cacccgagga cactcgcggc ggacatgccg
      781 atcccgggtg caatgccggc tcccgtgcaa ccgcccgagc cacgtggcag ccgctggcaa
      841 agcgagcgag tgagcgcagt tcggcacaat ttcgtgcgac cgccgccgcc cccggggctc
      901 gttaccgcga tgagggttca cgaaaaagtg atgtcagtga tgggcagatc gtgtaagacc
      961 ccgcgcgtca tgcgcattgc cccgctcgct gccgtcctcg ctgcgttcct ggtcgcgtgt
     1021 gttcacccca agccgggcta cttctcggcg caaaagcagg cacccgcggt gaagccggtt
     1081 gccccgacgg cccgaccttt cgtcaagatg ccgtgggtcg agctcgtcga tcaggtgcag
     1141 accggcccga tctatcgcgt ccagatcgcc gccgagatca cgcgcgacgt catcccgacc
     1201 gacgacctcg cgatcgtcgt ctcggtcgat gatcacgaag tcggccgcta tccgatcgtc
     1261 ggcaagtaca aaggtcgccc gacgaagatc aagcaagaga tcgagctgcc gctcggcgac
     1321 tacgagatcg attacgtcta ccaaggcgat cacttcgcgg gcatgccgtt ccgcctcgcc
     1381 gaggtcccgg tgtggggcgg ccgcacggcc ctccaactcc gcgcgcatca aggcacgcgc
     1441 gtttcgctcc gcgagaagaa gctgtgggtc ggacgttggt gggcgaacga cggtccggcg
     1501 caagcgtgga tcatcgaatg ggcgcgcgac ggccaagtca tcacgacgac gtccggcacc
     1561 gaagcggttg gcattccgcc cgaagcgcgt ggcctcgtcg gcggcgcggc gggtgcattc
     1621 cgtgccgaga agatcgtgca gaacacgatc tggacgtacg gcgaggagta cccgctcccc
     1681 gacgtcgtcg cgcaacagcc cggcgcatgg gccgcacgcg tcgtccacgg ctcgagcgcc
     1741 cccgtcgccg tcgtcttcac cgtcacgcag ctcggcacgc tcgtcgaagc gagctcgcgc
     1801 aaagtcatgc acgtcggctg ggagccgagc tggtcgaagc gcctcgatgc gcgctctctc
     1861 tcggtcaccg aagtcgatcg cctcaccgcg aagctgccgc acacgagctc gacgcaaccg
     1921 ttcgacgagc cgcgcaccga cggcatcgag ccggccgtcc gcgtgtcgcc cgccgccgtc
     1981 cgcgcgctgt tccgctcgaa gcagctcgcc gaatcgtggt gggagttcct ctcgctcaac
     2041 cgctcgacgc cagccactct caccgcgcag cccgccgcat tcgtgaaagg ctcgcagccg
     2101 gcaaacgctc gccgcgatgc cgccgcttcc gcgaagcaag atgccgagcg ccggacaaag
     2161 cttcgcaagc tgcgcgcgca gatcgacgag ctgatcaaga cgcagggctt cgcgtggaag
     2221 cccgacgagc acccgcacag ctagcgcggc cactcgcagc tgacgccgct acgcgctccg
     2281 tgcttcgacg cgcgtgaact taccccggtc gtttcaaact taccccggtc gtgtttcgca
     2341 gcagcgtctg tccgggttgt ccggctgtga tgcggacgag atgtccggca agcggacgtc
     2401 gtgcgtgtgt gttcgcgcag ttgcgctagg cgcgagagtt gctgtgctcg cggcgcatga
     2461 acatagaatc gattgatcgt agtgctgcgt atgtcgcggc gaagagcgcc gtcgatgtcg
     2521 tccagaagat cacgggcggc tggccgagcg aggtggcgga acctgcgaag cgctgcgcgc
     2581 agaacttgtt gtccgcgatt tcagaagcgc tggagcacgg cgagaccacc gcgtcgcggc
     2641 gacgctgttt gcgcggcgcg ctgctcaatg cgatcgagct cgcggcgatc tgcgacattg
     2701 ctcgagcgca cgggctcgtc accgagcaat cgctgcagtc gaccgggcgc acgctgtcgt
     2761 tgctcggcat gagctatcac gcgaccagcg tcgcgaacga atagcggcaa gcagtccgcg
     2821 tagccgaacg gacagcagcg acaagcagcg tctagccgaa cgcgaacgcg ggaatctgcg
     2881 agagatcgtc gatgagtgcg gcgcgtggat gcgccgtgac gaacgcgcgc tcgtaatctg
     2941 gtgtccgcga cgatgtgacg aggccgacgc gagcaccgag cgacagcggt agcgcgaggt
     3001 ccagctcgaa gatgtctccg acgacgacga gctcgtcgaa cgtcgagcca gcttccttga
     3061 cgacatcggc gaggatctcg aagtacgcgc gacgccgcag cagcacggcg cggtcgaggc
     3121 ctggaatcgc cagctcgctg gccgcgccgg tccatgcatc gtcgacgtcg aacttgcgcg
     3181 cgaaaccgcg gacgcgcgac gtgagccagg caacgcccgg cgcttcgcga tcgagggcgg
     3241 cgaccttgcc tgcgacggcg tgcgtatcgc tgttcgtcac gatccacgtc ggcgtgccgc
     3301 cgagcttgcg aagcatgtcg cctgcgccgg cgcggaagac ggggtggccg agcgtcttgg
     3361 cgtagttgta cttgtagagg acggagccga gcagccgccc gcgatcttcg tgcgatggga
     3421 tcgccttgta gcgatcgagg atgcggtgcg cgacgggcac catgcgcaga tatggatcga
     3481 cggttgctgg cgcgacggga cgccccatcc agaggaatgc gtgcgaggcc ggcgcggcgg
     3541 cgagctcgtc ttcgacttcg tcggcgatct tcgtgacgtc gaggagcgac gcgccggtga
     3601 gcagcgcgag gtcttctaga tagccgtcgc gaaacgggcg gccttcggct tcggcatccg
     3661 tcatcgtgcc gtcgaagtcc aggaccagcg ctactgccat cgcgggcgtg aacctaacac
     3721 gcgttcggtt attccgaacg acggtcgagc atcacttccg ggcgcgctac ttcgagcgct
     3781 gctcgtatac gccgtcccaa ccttccgggg gtgggcgcga cgcgaggtcg gcgcagcgtt
     3841 tcgccatcac ggcggcagtg gcgtcgtcgg agagtgcggc gaactgcgcg cgtgcggcat
     3901 cgaagtcgcg acggcggtac gtggcaagtg cctcggtgaa gcgctcgtcg actttgacgc
     3961 ccgcacgacc gacgagctcg tacaccggtg cagcacccgc acgacctttg acgcgcacga
     4021 ggtcgatctc gcggaacacg aagccggggc ccgcggcctt cgcggtcgcc tcaccgacga
     4081 ggatatcgac gccgtattcc ttcgtcagcg cttcgagccg cgcgccgagg ttcacttgat
     4141 cgccgagcac cgtgtaatcg aagcgcgcgg gcgtgcccat gttgccgacg gacatcgcgc
     4201 cggtgttgat cccgatgccg atcgcgactt cgggcttgcc ctcggtcttc catttcttgt
     4261 tcagcgcgat gagcgcttcc tgcatcttca gcgcgacttc acatgcgcgc gcggcgtgat
     4321 ccgggaggtc gacgggcgct gcccagatcg ccatcaccgc atcgccgatg tacttgtcga
     4381 gcgtgccgcc cgactcgagc accagatctg tcatcggcgt gaggtactcg ccgaggaagc
     4441 tcgctagcgt ctcgggaccc atgccttcgg cgaatgccga gaagccgcga atgtccgaga
     4501 acagcacggt cagctctttg cgctcaccgc cgagcttcat gc
//
LOCUS       DATLBZ010000464         8717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5052475, whole genome shotgun sequence.
ACCESSION   DATLBZ010000464 DATLBZ010000000
VERSION     DATLBZ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5052475"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VMZ53_23410"
     CDS             complement(<1..773)
                     /locus_tag="VMZ53_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31481.1"
                     /translation="MELPPAGLSWGEYVERWVDDCGGWLPLADQLIDRGQELVEIPLD
                     PQTVERGLRRLAKRAHKPGGQYGRWMLRFFGFTSPVEQWVKWLGTYHSRFADLPCSLR
                     LELLAMWNRPPISESPLICWIEHGIAIAHHSQLDFESCWHWLARAEKHAPAAGPAAEI
                     EVALFAAQLEVDAEDRVAVQRRYRLIEDRLRTATLPPADEIAYRARLADLRSRHFSRP
                     AAGDPADFDRARAHYETIPESSVPFASFRKYAGLAYCAWR"
     gene            complement(846..3242)
                     /gene="lon"
                     /locus_tag="VMZ53_23415"
     CDS             complement(846..3242)
                     /gene="lon"
                     /locus_tag="VMZ53_23415"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HUS31482.1"
                     /translation="MSQNLPVIPMRATVLFPGVSLPIAAGRSQTLRAIEAALRDPKRR
                     IFAVAQRDDGEDINADDLYTIGTIATIASVQRGQGGVLRLVLEGQTRAIAQRITNEGD
                     HLVAQVTEAVDQAPLDPKGAAFVALHREVRDRSGELAAKRGIPGEAIEQMLQQITEPG
                     ALADLVAGYLDIGITEKQQLLDMLAIEDRLRGVLIHLQKQLAVMSAQEDIQSKVKEEL
                     GDKQREVYLRQQLKAIQKELGEGDESGDKAMDELKAKLDALPLPDEARKEVDREWARL
                     TRVGRESMESQVIRTYLETIAELPWGTRTEELLDVASAAKILEEDHYGLKDVKDRVLE
                     FLAVHQLAKKDKGNKGRILVFTGPPGVGKTSIAKSIARAMGRKYVRISLGGARDEADI
                     RGHRRTYIGALPGRIIAGMKQAGSKNPVFLLDEIDKLGSSYHGDPAAALLEVLDPAQN
                     DTFVDHYLGVPFDLSEVMFIATSNFLQNIPGPLLDRMEVVDFAGYTEREKLEIAKTYL
                     VKRALEDNGLTGEQLTLTDDAIREVITGYTREAGVRQLERELGRLSRKVARKVAAKEV
                     ETTTLDRVDVRPLLGRPRVHPEKKAREDQIGISTGMYYTPAGGDIMFVEVATMRGKGS
                     LTLTGQLGDVMKESAQAAWTFARSHAELLGILDHHEFERDVHIHVPAGAIPKDGPSAG
                     IAMATAIVSALSRRPARHDVAMTGEISLSGRVLPIGGLKEKILGAVRAGIRDFVIPRD
                     NEADLDDLPTEVQDMIHVTPVDTLAEALAITLRDTTLRDGRLFFAEPLVSSIASQLAH
                     "
     gene            3499..3987
                     /locus_tag="VMZ53_23420"
     CDS             3499..3987
                     /locus_tag="VMZ53_23420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31483.1"
                     /translation="MCALVRRDHEDLDRALVAMVDPLTPPAELSNLLDVFRLALAVHS
                     AAEANVMDVLLERAGSPRTLSILAMQTRVEHAAQRAAAESLALLRPASIAWYSHALEL
                     RVLVLDHGSRSDHLRWSLEDLPSSVQHSLAGNYATQRLRVLASTSPLAIARKIETTRP
                     FH"
     gene            complement(3988..5256)
                     /locus_tag="VMZ53_23425"
     CDS             complement(3988..5256)
                     /locus_tag="VMZ53_23425"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUS31484.1"
                     /translation="MYAFLAAALIRYVTIGTDAFPEVHAADADARLVSSDGQVAVVEI
                     HEEDLEELSALMHEHHDRCGGYMVHDTLEEALAQDAPTRGPVEYTIDRGATVRWAIGH
                     IDAAQILKTIAELSSMQNRYYQSKHGAEASLWLQDKWQKLAAGRDDVTIELFDQGYPQ
                     KSVIMTIRGTTKPDEVIVIGGHLDSIALGGKAANAPGADDDASGVATLTEVARVLLQE
                     GYRPDRTIKFMAYAAEEVGLRGSLAIARDFQKRKVNVVGALQLDMTNYQGSDKDIWLM
                     KDFTDAKQNAFMVQLIERYVNATYGFDACGYACSDHASWTRIGVPASMPFESRMNERN
                     KQIHTKKDTLELSANNAQHAAKFARLGAAYAIELGKGGITNSGQPHSDAQLTLPDFHA
                     TKSSDSSSDTAAIVSLLLLVAGFAMSRRIV"
     gene            complement(5229..5552)
                     /locus_tag="VMZ53_23430"
     CDS             complement(5229..5552)
                     /locus_tag="VMZ53_23430"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HUS31485.1"
                     /translation="MEPTREEVDAMPGLTVLEFGATWCGFCQAAKADIERTVGARRDV
                     RHIWIEDGKGKRLGRSFGVKLWPTLVFLRDGREVARVVRPRGKSDLDKALLSFEACTP
                     SSPQP"
     gene            5601..6764
                     /locus_tag="VMZ53_23435"
     CDS             5601..6764
                     /locus_tag="VMZ53_23435"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HUS31486.1"
                     /translation="MKRVALLTLLAACGDDGGHTGLRDAKIFNDAPPPGNGQWVLGYY
                     VGYDINAAPIASIDWSALTHVAFAPLTVKSDQTLDFSFSDQQGMGATHAMELSAAAHA
                     NGVKALLMLGGAGAGANIAVAARPANRAAFVTRLVTALATYGYDGIDLDWEDSVVLDD
                     LVGLAHDLRAAKPDILLSYPAGSINGNIDTVDPRMATLAESLDRFMVQTYYPSTAFTG
                     SGWNSWFVAPLSGVSGATPIAADDTLMRYAAAGVPKAKLGLGIGAYAICYTGGITGPR
                     QPTNGTTQQITGGDNDLPLRTFFTSATFTNASAAAKQYDAAAQQPYLTFSSPVNEPGC
                     GGATQYISYEDPTSIAAKGTFSKQNGYGGVIIWTINELDPATFRALHDGFINP"
     gene            6986..7594
                     /locus_tag="VMZ53_23440"
     CDS             6986..7594
                     /locus_tag="VMZ53_23440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HUS31487.1"
                     /translation="MPTSWMLTRLKRNAQSEHVAANIDRLSLLHVEAELARYRDYLIR
                     IYGFEAPVEAALAATPGLDTVVDLGARSHLKMLRTDLAALGMPDVSGIRQCRNIPTHF
                     TSVGEALGWIYVIEHNATLHGQVRRHLHKRIGAQHDSYLLAGERSGSTRVQELGAVLD
                     RFAREPDTARQVVEAVKLAFRRQRQWFTQAMPPGDIRPTRVA"
     gene            7923..>8717
                     /locus_tag="VMZ53_23445"
     CDS             7923..>8717
                     /locus_tag="VMZ53_23445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31488.1"
                     /translation="MGADLVKVGEVVDGRYKILRPIADGGMGTVFLAEHWLIKRRVAM
                     KILHAELAHDATMIRRFMNEALAAGTLGHPNIVESTDMGFTKGDVPYIVFEYLEGAVL
                     ADEIMRRGRIPFSRALNIAYQIASALDAAHEAGIVHRDLKTDNIFLTKRPDTSDHVKV
                     LDFGISRFLLASDKTGLGGNLLGTPEFMAPEQVTTPDTVDKRADIYAIGVVLFEMLMG
                     HVPFKLEPQAPGKLDVEAAHRLLDRIINEEPMALECRDAPAGFGHMV"
BASE COUNT         1506 a         2891 c         2779 g         1541 t
ORIGIN      
        1 cgccacgcgc agtacgcgag gcccgcgtac ttccgaaacg acgcgaacgg cacgctcgac
       61 tcgggaatcg tttcgtaatg cgcacgtgcg cgatcaaaat cggccgggtc tcccgcggcg
      121 ggccgcgaga aatggcgcga gcgaagatcg gcaaggcgtg cgcgatacgc gatctcatcg
      181 gcgggaggca gcgtcgcggt ccgcagccga tcctcgatga gccgataacg ccgctgcacc
      241 gcgacgcgat cttccgcatc cacctcgagc tgcgctgcga acaacgccac ttcgatctcg
      301 gcagcaggtc ccgcagccgg cgcgtgtttc tccgcgcgcg cgagccagtg ccagcatgat
      361 tcgaagtcga gctggctgtg gtgcgcgatc gcgatgccgt gctcgatcca gcagatcagc
      421 ggcgattcgg agatcggcgg ccggttccac attgcgagga gctcgaggcg aaggctgcac
      481 ggaagatcgg cgaagcggct gtgataggtg ccgagccact tcacccactg ctcgacgggc
      541 gacgtgaagc cgaagaaacg gagcatccag cgcccgtact ggccaccggg cttatgcgcg
      601 cgcttcgcga ggcgacgcag gccgcgctcc accgtctgcg gatcgagcgg aatctcgacg
      661 agctcttgcc cgcgatcgat caactgatcg gcgagcggga gccaaccacc gcagtcgtcg
      721 acccagcgct ctacatactc accccacgaa aggcccgccg gcggcaactc catgcggagg
      781 actagaacat gagtcgccga ccggcggaat ccagaggcgt ttgtgcgtgc gctggcttac
      841 gcgagctagt gcgcgagctg gctggcaatc gacgacacga gcggctcggc aaagaacagg
      901 cggccatcgc gcagcgtcgt gtcgcgcagc gtgatcgcga gtgcttcggc gagcgtgtcg
      961 acgggagtca cgtggatcat gtcctgtacc tccgtcggca gatcgtcgag gtcagcctcg
     1021 ttgtcgcgcg ggatcacgaa gtcgcgaatt cctgcgcgaa cggcgccgag gatcttctcc
     1081 ttgaggccgc cgatcggtag cacgcgcccc gacaacgaga tctcgccggt catcgccacg
     1141 tcgtgccggg ccggccgacg agacagtgca gacacgatcg ccgttgccat cgcgatgccc
     1201 gcggaagggc cgtccttggg gatggcgcct gccggaacat ggatgtgcac gtcgcgctcg
     1261 aattcgtggt gatcgaggat gccgagcagc tcggcgtgcg accgcgcgaa cgtccacgcg
     1321 gcttgcgccg actccttcat cacgtcgccg agctgaccgg tgagcgtcag gctgcctttg
     1381 cctcgcatcg tggcgacctc gacgaacatg atgtcgccgc ccgcaggcgt gtagtacatg
     1441 cccgtcgaga tgccgatctg atcctcgcgc gccttctttt caggatggac gcgtggacga
     1501 ccgagcagcg gccgcacgtc tactcgatcg agcgtcgtgg tctcgacttc cttcgcggcg
     1561 actttgcgcg cgaccttgcg agacagccgg ccgagctcgc gctcgagctg gcgcacgccc
     1621 gcttcccgcg tgtagcccgt gatgacctcg cggatcgcgt cgtcggtgag cgtgagctgc
     1681 tcgccggtga ggccgttgtc ctcgagtgcg cgcttcacga ggtaggtctt cgcgatctcg
     1741 agcttctcgc gctcggtgta accagcgaag tcgacgacct ccatacgatc gagcagcggg
     1801 cccggaatgt tctggagaaa gttcgacgtc gcgatgaaca tcacctcgga cagatcgaac
     1861 ggcacgccga ggtagtgatc gacgaacgtg tcgttctgcg cggggtcgag aacctcgagc
     1921 agcgcagcag ctggatcgcc gtgatacgac gagccgagct tatcgatctc gtcgagcaga
     1981 aacaccgggt tcttgctccc tgcttgcttc atgccggcga tgatgcggcc cggcagtgca
     2041 ccgatatagg tgcgacgatg acctcgaatg tcggcctcgt cgcgcgcgcc gccgagcgag
     2101 atgcggacgt acttgcgtcc catcgcgcga gcgatcgact tcgcgatcga cgtcttgccg
     2161 acgcctggcg gacccgtgaa gacaaggatg cgacctttgt tgcccttgtc cttcttcgcg
     2221 agctgatgca ccgcgaggaa ctcgagcacg cgatccttca cgtccttcag gccgtagtga
     2281 tcctcctcga ggatcttggc ggccgacgcg acatcgagca gctcttcggt gcgagtgccc
     2341 cacggcagct cggcaatcgt ctcgaggtac gtgcggatga cttgcgactc catcgactcg
     2401 cggccgacgc gcgtcaggcg cgcccactcg cgatcgactt ctttgcgcgc ctcgtcggga
     2461 agcggcagag cgtcgagctt cgccttgagc tcgtccatcg ccttgtcgcc ggactcgtcg
     2521 ccctcgccga gctccttctg gattgccttg agctgctggc gcaggtagac ctcgcgctgc
     2581 ttgtcgccga gctcttcctt cactttcgac tggatgtctt cctgcgccga catcacggcg
     2641 agctgcttct gcagatgaat gagcacgccg cgcaggcggt cttcgatcgc gagcatgtcg
     2701 agcagctgct gcttctcggt gatcccgatg tcgagataac cggcaacgag atccgcgagc
     2761 gcgccgggct ccgtgatctg ctggagcatc tgctcgatcg cttcgccggg aatgccgcgc
     2821 ttggcagcga gctcgccgga gcgatcgcgc acctcgcgat gcagtgccac gaatgcagcg
     2881 cccttcggat cgagcggcgc ctgatcgacc gcttcggtca cctgcgcgac gaggtgatcg
     2941 ccctcgttcg tgattcgctg cgcgatcgcg cgagtctgac cttcgagcac gaggcgaagc
     3001 acgccgccct gcccgcgctg gaccgacgcg atcgtcgcga tcgttccgat cgtgtagagg
     3061 tcatcggcgt tgatgtcctc cccatcgtcg cgctgggcga cggcgaagat gcgccgcttc
     3121 ggatcgcgaa gtgccgcctc gatggcgcgc agcgtttgcg aacgcccggc ggcaatcggc
     3181 aggctcacgc cagggaacag caccgtggcg cgcatcggaa tcacagggag gttttgcgac
     3241 ataccgttgc aagtgggtac cgtcaggtct cgtcgcaacc cgcccgaaag actcgcggcg
     3301 gttccgacac tggccgaccg actaggccgg agggcacgcg catgcgggct cgacgtgcgg
     3361 tgtcgcgcgc tccatggagc aacctcacga gtgcaactag ctgtgagcac gtgcaccgcc
     3421 ggatagaccg taggctcact caccagcgat tccttcggca ctttcagtga acgcgttctc
     3481 ggcgggatga tgctcgacgt gtgtgcattg gtgcgacgtg atcacgagga cctcgaccgc
     3541 gctctcgttg cgatggtgga ccctctaact ccgcccgcag agctctcgaa cctcctcgac
     3601 gtgttccgac tcgcgttggc cgtgcattct gcggcggaag cgaatgtgat ggacgtgctc
     3661 ctcgaaagag caggctcacc gcgcacgctc tcgatcctcg cgatgcagac ccgcgtcgag
     3721 catgcggcgc agcgagcagc agccgagtcg ctcgcgctcc tacgaccggc gagcatcgcg
     3781 tggtacagcc acgcactcga gctacgcgtg ctcgtgctcg atcacggctc gcgatccgat
     3841 cacctgcgtt ggagcctcga ggacctgccc tcctccgtcc agcacagcct cgccggtaac
     3901 tacgcgacgc agcgcctgcg ggtgctcgcg agtacctcgc cgctggcgat cgcccgcaag
     3961 atcgagacga cgcgaccgtt ccactgatca gacaatgcga cggctcatcg cgaagcccgc
     4021 gacgagcagc agcaacgaaa cgatagccgc cgtgtccgag ctcgagtcgg aagacttcgt
     4081 cgcgtggaag tccgggagcg tcagctgtgc gtcggagtgc ggttggccgc tgttcgtgat
     4141 gccgcccttg ccgagctcga tcgcgtaggc ggcaccgagg cgggcgaact tcgccgcatg
     4201 ctgcgcgttg tttgcggata gctcgagcgt gtccttcttg gtgtggatct gcttgttgcg
     4261 ctcgttcatg cgcgactcga acggcatcga ggccggcacg ccgatgcgcg tccagctcgc
     4321 gtgatccgag caggcgtagc cgcacgcgtc gaagccgtag gtcgcgttca cgtacctctc
     4381 gatgagctgc accatgaacg cgttctgctt cgcgtcggtg aagtccttca tcagccagat
     4441 gtccttgtcg gagccctggt agttcgtcat gtcgagctgc agcgcaccga cgacgttgac
     4501 cttgcgcttc tggaagtcgc gcgcgatcgc gagtgagcca cgcaagccga cctcttcggc
     4561 ggcgtacgcc atgaacttga tcgtgcggtc ggggcggtag ccctcttgca gcagcacgcg
     4621 tgcgacttcg gtcagcgtcg cgacgccgga cgcatcatcg tcggcaccgg gcgcgttcgc
     4681 agccttgccg ccgagcgcga tcgaatcgag gtggccgccg atcacgatca cttcgtcggg
     4741 cttcgtcgtg ccgcggatcg tcatgatgac ggacttctgc ggatagccct gatcgaacag
     4801 ctcgatcgtg acgtcgtcgc gcccggccgc gagcttctgc cacttgtcct gcagccacag
     4861 cgacgcctcg gcgccgtgct tcgactggta gtagcggttc tgcatcgacg acagctcggc
     4921 gatggtcttc aggatctgcg ctgcgtcgat gtggccgatg gcccagcgca cggtagcgcc
     4981 gcgatcgatc gtgtactcga cgggaccacg cgtcggcgcg tcttgcgcga gcgcctcctc
     5041 gagcgtgtcg tgcaccatgt aaccgccgca tcgatcgtga tgctcgtgca tgagagcaga
     5101 cagctcttcg aggtcctcct cgtggatctc gacgacggcg acttgcccgt ccgaggaaac
     5161 gagccgcgca tcggcgtcgg ccgcgtgcac ctcgggaaac gcgtcagtcc cgatcgtgac
     5221 gtagcggatc agggctgcgg cgaggaaggc gtacatgctt cgaagcttag cagggctttg
     5281 tcgaggtcac tcttgccgcg tgggcgcacg acccgagcga cttcgcggcc atcgcggagg
     5341 aacacgagcg tcggccacag cttgacgccg aacgagcggc cgaggcgctt gcccttgccg
     5401 tcctcgatcc agatgtgacg aacgtcccgg cgtgctccga ccgtccgctc gatgtctgct
     5461 ttggcggctt ggcagaaccc acaccacgtg gcgccgaatt cgaggacggt cagacccggc
     5521 atcgcatcta cttcttcgcg tgtcggctcc atgatcgagc ttagcaaccc accgccgatt
     5581 gtgtgaccat cagaaaacag atgaagcggg tggctcttct gacattgctc gcggcatgtg
     5641 gagacgacgg cggacacacg gggctccgcg acgcgaagat cttcaacgac gcgccaccgc
     5701 ccggcaacgg ccagtgggtg ctcggctact acgtcggtta cgacatcaac gcggctccca
     5761 tcgcatcgat cgactggagt gcgctgacgc atgtcgcgtt tgcgccgctg accgtcaaat
     5821 cagatcagac gctcgacttc tcgttcagcg atcaacaggg catgggcgct acacacgcga
     5881 tggagctgtc cgccgccgcg cacgccaacg gcgtcaaggc actgctgatg ctcggcggcg
     5941 ctggtgcagg cgctaacatc gcggtcgctg cacgcccggc caaccgcgca gcatttgtca
     6001 cgcgactcgt cacggcgctc gcgacctatg gctacgacgg catcgatctc gattgggagg
     6061 acagcgtcgt gctcgacgat ctcgtcgggc tcgcccacga tctgcgcgca gcgaagcccg
     6121 acatcctgct gtcgtatcca gcgggcagca tcaacggcaa catcgacacc gtcgatccgc
     6181 ggatggcgac gctcgccgag tcgctcgacc gcttcatggt gcagacgtac tacccgtcga
     6241 ccgcgttcac ggggtcgggc tggaattcgt ggtttgtcgc gccgctctcc ggcgtcagtg
     6301 gtgccacccc gatcgcggca gacgacacgc tcatgcgtta cgccgcggcg ggcgtgccga
     6361 aagccaagct cgggctcggc atcggcgcct atgcgatctg ctacacgggc ggcatcaccg
     6421 gcccgcgcca acccacgaac gggaccacac agcagatcac cggtggcgac aacgacctcc
     6481 cgctccgcac cttcttcacc tccgcgacgt tcacgaacgc gagtgccgcc gccaagcaat
     6541 acgacgctgc tgcccagcag ccctacctga cgttcagctc acccgtgaac gagccggggt
     6601 gcggcggagc gacccaatac atctcctatg aggaccccac gtcgatcgcg gcgaagggca
     6661 ccttctcgaa acagaacggc tacggcggcg tgatcatctg gacgatcaac gagctcgatc
     6721 cggccacgtt ccgagcgctg cacgacggtt tcatcaatcc gtagcgagcc cgatctgtgc
     6781 caacggcgca catcccgggg gattgcgaca atcgggcgca gttgcgtctc gtcggcgccc
     6841 ggaaagaggg atcttgccgt gccctagcgc gctttcgccc gagcggtctc gcacaccgtg
     6901 cccgataaca acagctcgcg cggcaaagtg ctgagatccg cgttgttcgg ttagagcacg
     6961 gtcgttgcag agtgggctgt catccatgcc gacctcgtgg atgttgaccc gcctcaagcg
     7021 caatgcgcag tcggaacacg tcgccgcgaa tatcgatcgc ttgtcactgc ttcacgtgga
     7081 agcagagctg gcgcggtatc gcgactactt gatccggatc tacgggttcg aagctccggt
     7141 ggaagcggca ctcgccgcca cgccgggcct cgataccgtg gtcgacctcg gcgcgcgttc
     7201 gcacctcaag atgctgcgga ccgacctcgc tgccctcgga atgcccgatg tcagtgggat
     7261 ccgtcagtgc cgcaacatcc ccacccattt caccagcgtg ggtgaagcgc ttggctggat
     7321 ctacgtgatc gagcacaacg ccacgctcca cggccaggtc cgtcggcatc ttcacaaacg
     7381 catcggggcg cagcacgact cgtatctgct cgccggtgaa cgcagcggct cgactcgcgt
     7441 gcaggagctc ggcgccgttc tcgatcggtt cgcgcgcgag cctgatacag cacgtcaggt
     7501 tgtcgaggct gtgaagctcg cgttccgtcg ccagcggcag tggttcaccc aagcgatgcc
     7561 gcctggtgac atacgcccga caagggtcgc ctgaccactc ggaggcacag tgcattgccc
     7621 cgcggaaaca gaactttgcg aacagtgcgt cgcagagctg gggaggctac gtccgcgagc
     7681 agttgaagcg gggacgcatc tcgccgatct actcgcaagt cacgtcgtgc cggaccaacc
     7741 ctggctcgat acgctcgacg cgaagagcga gcccatctgc cggcgcgtcg cagcgttcac
     7801 gacggacgac cgcatcgcgc atgagctctg ccaaatctgc gtcgagagtg cgcgcctgag
     7861 gtgggagcag ttgcgacagc tgcggagcct gcgcagatag agccggtata cactcgtcgt
     7921 cggtgggcgc agatctggtc aaggtcggcg aggtcgtcga cggtcgctac aagatcctgc
     7981 gcccgatcgc ggacggcggc atgggcaccg tgttcctcgc cgagcactgg ctcatcaagc
     8041 gccgcgtcgc gatgaagatc ctgcatgcgg agctcgcaca cgacgcgacg atgatccgcc
     8101 gcttcatgaa cgaggcactc gccgcgggca cgctgggtca tcccaacatc gtcgagtcca
     8161 cggacatggg cttcacgaag ggcgacgtgc cgtacatcgt gttcgagtat ctcgagggcg
     8221 cagtgctcgc tgacgagatc atgcgacgtg gccgcatccc gttctctcgc gcgctcaaca
     8281 tcgcgtacca gatcgcgtct gcgctcgacg ctgcccacga agccggcatc gttcatcgcg
     8341 atctcaagac agacaacatc ttcctcacga agcgccccga cacgtcggat cacgtgaagg
     8401 tgctcgactt cggcatctcg cgctttctct tggccagcga taagacgggc cttggcggca
     8461 acctactcgg cacgccggag ttcatggcgc ccgagcaagt cacgacgccc gacaccgtcg
     8521 acaagcgcgc cgatatctac gcgatcggtg tcgtgctctt cgagatgctg atggggcacg
     8581 tgccgttcaa gctcgagcca caagctccgg gaaagctcga cgtagaagcc gcgcatcgcc
     8641 tgctcgatcg aatcatcaac gaggagccga tggcgctcga atgcagggac gcgcccgcag
     8701 gcttcggcca catggtc
//
LOCUS       DATLBZ010000465        60071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7740203, whole genome shotgun sequence.
ACCESSION   DATLBZ010000465 DATLBZ010000000
VERSION     DATLBZ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 60071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60071
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7740203"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VMZ53_23450"
     CDS             complement(<1..712)
                     /locus_tag="VMZ53_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31489.1"
                     /translation="MPRTAPTTDWTPDPAAAGSEQPAPTEPETRAGKLWARIVWLASW
                     LLPPTLMAGAYTILALTSDTGAFGKSFMALGFGFVIVLWLVFRTAAESAGISRALSGG
                     DADSLLAISTKQLRRRRRSSDASRAPFLIYKALAHELRNEPTDALAAATEAKPSREHH
                     ALLALAVRITSLVDLGRAAEAHTLAADLEALAARVDRRLQPMPHHYAHLARARLLAAD
                     AKLTEANAELSKVIDDVRA"
     gene            complement(729..2120)
                     /locus_tag="VMZ53_23455"
     CDS             complement(729..2120)
                     /locus_tag="VMZ53_23455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HUS31490.1"
                     /translation="MTTARQSSPVLPKMYDSPDDWTPETWRARPAAQQASYGDEAKLA
                     RVMQEMAALPPLVTSWEVENLKADLAKAARGDAFVLQGGDCAESFDDCRSDPIAAKLK
                     ILLQMSLVLVHGTRKPVVRIGRIAGQYAKPRSAENETRGEVTLPAYRGDIINRDAFTA
                     EDREPDPTLMLRAYERAALTLNFIRALVDGGFADLHHPENWDVSFAAGSPNAAQYERI
                     VGSIRESLDFVSAITGVENEVLRRVTIYTSHEALALPYEQAQTRRVPRRNGYYNLSTH
                     FPWIGMRTAQLEGAHVEFHRGIRNPLGIKVGPAMTIGWLLGLLDVLDPQREPGRITLI
                     HRMGADNIQEKLPALVEAVQRTGRRVLWICDPMHGNTETTPQGIKTRRFDKIVNELEQ
                     SFELHTRLGSRLGGVHIELTGENVTECVGGARGLVEADLAHAYKSRVDPRLNYEQALE
                     TALRISRHLRSGT"
     gene            complement(2120..3856)
                     /locus_tag="VMZ53_23460"
     CDS             complement(2120..3856)
                     /locus_tag="VMZ53_23460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HUS31491.1"
                     /translation="MKSLVVCLVLVAACSQPATKHVDHGPPVAKKGARTLTIIGTNDL
                     HGAIDRLPLLAGFINNVRAAREADGGGVLLVDAGDMFQGTLESNLAEGEDVVRAYNAI
                     GYTAAAVGNHEFDFGPEGPSVVAKSVEEDPRGALKARAAEAKFPFLVSNINDQKSGNR
                     IKWPNMPSSILVEVAGTMVGVIGAATESTPFTTMPANFTGLVMAPTAQAIAKEAEALR
                     ARGAQVIVVVAHIGSACKDVEKPNDLSSCKTDEEVFKVISDLPKGLVDVWVGGHTHAT
                     VAHRIKDVAVIESYSSGRAFGRVDLRITDTHVSAVQIHKPQLLCPLDSEGNPPPAADC
                     HPEAYEGKPVVPDADVQKIADEASAKAAPRRDEKLGVQLTAIVTKSYSLESAEGNWFT
                     DLMLAARPDAQLALTNGGGLRADIPAGELTYGRLYEAMPFDNRFAIVEMKGKHVRRLV
                     TTNIQRGSGIYSWGGLVAKARCKSGQLDVEIKVAGKPIADEASYKVVTSDFLASGGDM
                     SVIGRLKLPEGAIQMTDVIIRDAIADVLRKKKGKLDPATLYSPAKRRLDYEGERPVEC
                     GGKQQKQQQEPD"
     gene            complement(3870..4358)
                     /locus_tag="VMZ53_23465"
     CDS             complement(3870..4358)
                     /locus_tag="VMZ53_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31492.1"
                     /translation="MKAECAELVGMNAQCEASLADGTKLPIDVTTEKGQWSWRLKGVV
                     VEQPALTEFVEKALAELHVDQKVDCTPVVHVVQPGERTTCKLSGGGLAFVQIAADGNA
                     RLELELDAAAAKARGEIVTPERDRELIKISRDLEGGAGDSDGEEEVTGDGGVGPAGDG
                     GV"
     gene            4597..5979
                     /locus_tag="VMZ53_23470"
     CDS             4597..5979
                     /locus_tag="VMZ53_23470"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31493.1"
                     /translation="MRNQLARWLLTASLLGGSLLTTASAQPNVRDHRKGHGPKIDAKI
                     DAKVVVDVNPRTAPPPPKAEKAGSRGGFVWVAGNWDWQKGQWVWVSGHWEKHRKGKRW
                     RDHRWEKQGDVYVRINGDWQDDRPNAAPPALQEEKWEPRTGFVWVRGNWDWQNGNWTW
                     VAGHWERERAGKRWTEGQWVLQGGAWVWTDGSWADAPKFPTAAPPAIKVETVAAKPGY
                     LWVKGKWDWRNGEWAWTPGHWEKERAQYSWQDGRWEQKNGQWTWVEGSWVAAAAPPPV
                     VISVVPTAAPPAPQAETVSPRSGFIWVRGRWDWQKGNWVWVAGHWERERAQSKWIDGS
                     WQNQGGKWVWVEGSWQTSAPPAPAPTPAPAPTPAPTPAPPPAPPVAQTPPPGPTTAPP
                     QPQAENVAPRSGFVWVKGHWGWSANNKYEWIGGHWEREKANVKWYDGHWESKSQGNVT
                     YWVWIEGGWK"
     gene            complement(6049..6504)
                     /locus_tag="VMZ53_23475"
     CDS             complement(6049..6504)
                     /locus_tag="VMZ53_23475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31494.1"
                     /translation="MRAAAVALLLVGTTAVASAEDELALTSRWHDVPTGHQLKLSQQI
                     TDQLTELGNFIGGHVNVLSDDVMGLRFDGRKRRARFRLGTGEGQYLRFKLDTDWHFAA
                     GKARVAARVELGLGTHEWHLELPDVEMLPSSVYGERGVEVRLPIFERRW"
     gene            6528..7487
                     /locus_tag="VMZ53_23480"
     CDS             6528..7487
                     /locus_tag="VMZ53_23480"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HUS31495.1"
                     /translation="MKLRNAGILVALLVAVGACKKSEPQAQKAGSGVAQVTVGSADGS
                     SAATDPWAKPKVAKDPLAKPLFWSLEKDGKTDYILGTMHTGVDPEARLPDIVWKKLDE
                     AKTFAMETNLADAGKLEVLRKDGTSLEDELGPEYWKKLEAAIGVQEASRLRGFKPMIP
                     ATLLALRGLPETPPMDGALHGRALNQHKEIVFLEDITAQGSVLEKWMNTKALKDILDD
                     LEIGEKRSKEMLAAYISGDEARIEAISNEEREDFKRHGRSEKEYDEQMEDLLYKRNAS
                     WIPILEKIHGNAFIAVGAMHLIGKRSVLDLLQQQGYKVTRITP"
     gene            7571..8590
                     /locus_tag="VMZ53_23485"
     CDS             7571..8590
                     /locus_tag="VMZ53_23485"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31496.1"
                     /translation="MHRLALSTFALVLFSTTTACGKDPSTGDDGVDAGFTGDSFSLKW
                     GPVDVAPGKEDTQCIWVRLNNETEIKVHGMHNVLSASSHHLIVYKDDMDTTEQTTPVP
                     CEPFTGALNTTGMVAPIAITQKADDKIYLPDAVAYTFAPHQMVKLEMHYLNASDASEN
                     ATADVDFYVAEPSTIQYEASILFTGSMDISLPPGQTTTLHQFFAVPSYLDLSKSNIFA
                     ITGHTHALGTDVKVGIGPSKTGPFSEVYAPSPFSWSEPETRTFDDPFSIPVGGGMDFS
                     CTWNNTKPTTVTFGESATKEMCFFWAYYYPSQGSKVCIHTDKYNGLNVCCPGDSLCSY
                     VEGML"
     gene            complement(8868..9590)
                     /locus_tag="VMZ53_23490"
     CDS             complement(8868..9590)
                     /locus_tag="VMZ53_23490"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUS31497.1"
                     /translation="MPPRTEIETVELAGLTTRIVGNGPITCVLLHGFGAPGDDLVALA
                     QYIPLPAATGGAAGVRFVFPAAPIELGGLYGDSRAWWHLDLARLEQDLRTGAIRDRRG
                     EIPTGLAEVRTQIDALLSALKDRYAVDDSKLVLGGFSQGAMLALDVALHRATAPAAVI
                     LMSGTLIAESEWRPLYAKLANTPILQSHGRADSLLPFMIAELLRDDLTAAGAKVEFIP
                     FNGGHEIPPPVLLQAGKLLAQL"
     gene            complement(9647..10597)
                     /locus_tag="VMZ53_23495"
     CDS             complement(9647..10597)
                     /locus_tag="VMZ53_23495"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS31498.1"
                     /translation="MAQRPPETPSSSGKKPRGFPWRLWLYALVMTAGAVAGGYFTWQY
                     RSTGKTAISDYDKCSKTLKTAQDAGSKHDKEVKTCTDALGAATLKVTELEKSNTEGYK
                     NLTASQAELVALRAQKAEADKRMAAIEDIRKQFAKMIDTGQLHVSARRGQLVISLPSE
                     VLFQTGSADLSKQGEYAVVEVASVLKKLPDRRFLVVGHTDNVEYVKPKGADAAAGGSA
                     ASAACGIPTDNWTLSTARALTVTRVLVTAGMDAKSLVPSGVGANDPIASNATNDGKQK
                     NRRIEIALLPAINELPPMPANLSDETAPAGATTPVDAPKK"
     gene            complement(10661..13930)
                     /locus_tag="VMZ53_23500"
     CDS             complement(10661..13930)
                     /locus_tag="VMZ53_23500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31499.1"
                     /translation="MGTGRSYLAAFASVHTLANAEERRQVTRQGLAMLAEIAEREPAP
                     LEGLASDQLLLAVRTALADGMLGDLDWLSPASAAIATFELAQALPNGAERRELGRRVL
                     TRLREADRDTFVRLLISLARTSPKIVASEGLRARLEVVLAAPLTAPGMIGELAIGLLA
                     QPALAQSWVEVPATGSLPGRRLSARLLAHGAREAVRRYDSGDRGGVRVLARAGLREAL
                     ARLLGDREALVWRFASIARGILAHVDSALADDIDRELRPSATNTEQRRAAASAAAALE
                     RGGGARRWLDVLVERAQKEPGIARGTILGLAGLAIVAPDDADVLATKLVARAPLEAAE
                     ALAELRREEAAPLLPDATAMALDWTRDQMKGDATSIDDSRFALLYALEAELGGAQAAE
                     GLAVPINAARNALDLGDVKTALREARIAVDEIGASADWLERASDDDPVDRRHSMRLLR
                     ELDRELLADNALPAVLALSGDTDPARQQFAKALASIERSLLDRELRPEAGAVAHGGLR
                     IARLRALVRLLDGVRVSGESDLEPRLAAVRQLMKRAADDQSSLRRAVWAAMTRAGDAL
                     LRDGHAEVTDLLLAWTLAFPDDDFAIVREASMVPDIEASFAAYAQLNQATWEAADPDD
                     AEAVRTVVERLGELADALPPEQSPRVESVRLALARLGNQLTRLMSSTSQEAVETGMLD
                     SIATELGALARRTYGARRRLGLDAGDLANELETAVRAIETVLAARASQRSDDAPRDSS
                     SQLDEAIAQTIDHARSALPPALAAAIERVLVWLARRPIDRTEERGASSSDVSLPGWVP
                     LSRLLGGFYVVRPIGRGAGGSVLLAVRAEHRNRPDRELVALKVPDYSGDAARAMSEQE
                     FERLFREEAGALLALPAHPNLARFITFDASAQPKPLLAMEYVRGTNLERTLESNLTMR
                     RAVDIVDDLLAGLEAMHAVRIAHLDVKPPNLVLREGSGRAVLVDFGLAGRHIRVGCGS
                     AHYGAAEVWSEDGTTFEPFGTDVYAAACVAFECFTGTLLIQGENLQQLLDQHYAAQPG
                     AEVLARMSARDPRVAPLADLLRAAVIRDPRKRPSVSRLRAGFAAIAPDLRTLDWPIST
                     S"
     gene            complement(13950..15212)
                     /locus_tag="VMZ53_23505"
     CDS             complement(13950..15212)
                     /locus_tag="VMZ53_23505"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HUS31500.1"
                     /translation="MTPTRRDVLRWGGLGTAALVTGCGDNDLVRDPGTQLATAIVEPE
                     SDSFLVAVWAELARDIALEVQVGDTTVLQTAATLDAARRATFDVGGLAPGSLYRVRAL
                     ADTGAELVHHVRTAPSPDDTRPVRIAVSADVDPYPEFDSELVEHVIAASPDVFVSIGD
                     FPYTDNGPPAMTLDTYRTRHVEARTSQRVRRLLTQMGLRAIYDDHEFRNDWDAMYRET
                     EADRYVAAMQAWDEFFPLRRTDIRYRSWRWGAHVECFLLDTRQFRSANAARDNDAKTM
                     LGETQLAWLLDGVRASTAPFKLVFTSVPLDFGDGNDHWSSFTTERARMFDAFAGVPGV
                     LFVSADQHWFAAHRHERGIREFQVGPISRGIGTPMRTADGVLFRHRAFNVGLVDIDGE
                     SLTFSGLGADGTVFYKETLTVEDLTPAT"
     gene            15262..16182
                     /locus_tag="VMZ53_23510"
     CDS             15262..16182
                     /locus_tag="VMZ53_23510"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HUS31501.1"
                     /translation="MRRVLGCVGLSGCLASVLAGCAFEHGDLGGSDDDPGSGSGSGAV
                     QPGDFDGDGVADLDDNCNTAANADQRDHDDDGRGDRCDVCPHLPDAGGDSDGDGVGDA
                     CDPNPTTASDRIAFFDGFYQMPQWDNVQGPGMWQVNSGVLRQPKLEASQLVRDDDPDL
                     GTVFVDARVQVNSMAMGPAARRSVGLVVGFGDAKHYFFCGVAQTQLGSEVQAGAEFTD
                     FWGTAQYEYAPGAFASQVGGGWLTLQTSTSVSSWGGTHIDCMGHRGQVTGTAQFEHDG
                     TPTGDIGLRTNGIDASFDYVFVVETAPSGS"
     gene            16220..16819
                     /locus_tag="VMZ53_23515"
     CDS             16220..16819
                     /locus_tag="VMZ53_23515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31502.1"
                     /translation="MQPHKESRGRTNANARNQREREAMREANRMPQPTIADIDPEDPT
                     QRIDDAGHYSITDLDVEAQPSAEAAADIDTAVADARGEADGEDNDEEAMIGEPDETTA
                     DAYAAGVKKDSGELYGIHTPHAADNDLDKTDDQESFKDSEQGEHWLETLGKKAAEYGA
                     AAEEELDVVDNSDHDGGHHSSEGGDRPVADKGSGGPGGL"
     gene            complement(17111..18016)
                     /locus_tag="VMZ53_23520"
     CDS             complement(17111..18016)
                     /locus_tag="VMZ53_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31503.1"
                     /translation="MKRALVLLLVAACGEVDEHRHLDAGADGSSADAAPDARTPVCWG
                     APFSTAPTAIAALNTNNRDAFLRLSPDELTAYFSSDVVGAATVLGLWKSTRASTSATF
                     AAPARLLLTGDNAVEVWSPTVTANGLTLYFTRTNGNSQKDIYKATRGAITDANFSGVA
                     LVAELNTASSEEDVYVVPDGSAIYFSSDRGNGNTLSIFRAQLNGSTFSPPTEVLADTN
                     LFVSRVVISPDELTMFYQDANEIHETRRASKTAPWTLEPALTVLGSPNADHPTWISPD
                     GCRLYLESDRGSAPLDLYVAVRSPQ"
     gene            complement(18031..18870)
                     /locus_tag="VMZ53_23525"
     CDS             complement(18031..18870)
                     /locus_tag="VMZ53_23525"
                     /inference="COORDINATES: protein motif:HMM:NF012406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HUS31504.1"
                     /translation="MDNVRLRRLKSDYEALRRLAHLHPKIEIEGVQGNPPDRYRLKLK
                     VKSIREKGDEIEMVQEHRLEVTMPRGYPRDAPMFRMLTPVFHPNVAPHAVCIGDDWTA
                     SEALDTLIQRVGEILAYQSYNTKSPLNGRAAQWVDENKHKVPIDKEEFFVDLNEAATS
                     MNAMAAANVCSNCAAQNVALKQCDKAQHKICSDCEMHCGTCGRLLCLTCGERTCTACQ
                     SQCANCKTPTKREQMFACTQNHQLCRDCCFQCQTCKDILCLMCNTVPCPRCQPSPQPR
                     SHI"
     gene            complement(18873..20186)
                     /locus_tag="VMZ53_23530"
     CDS             complement(18873..20186)
                     /locus_tag="VMZ53_23530"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HUS31505.1"
                     /translation="MVELSDAAPQKKKRFWRKSEAPIATSRVCPKCNVAGPKKLAPGQ
                     ICDSCEAQQAWNAIGESGIKIDTKAIQDAVTRREGEAAGEPLWQKALGFAPVLLSLAV
                     AAFAGWMIYLLLRPREIGPLKDLFDDLSSSSTRALWIGIGTFIVGIVALVRTRKQRSY
                     RKAHVLIAHLLAIVAGLGSAVIGGFHWYAMPSSFGGKYSSMPPKEQLGFASTSTVDRI
                     VAATVVVFAPDASGDARALAMGTGAIVGGDAKRAWIVTCSHVAMPYAAVGSWRHAKDA
                     QPVWVQLSDARQGKAYVRWAAPPPLDIAVIEFEIDNPPPPVQIAPNAGALEASASVMF
                     VPNPLRDGWLVHKGHLMQKEPHNTPAGTYELLITDLPVTHGDSGSGLFDDRGQLVGLN
                     TWTKVEGGLAHGISLPSEAMRALSDAVQNHELDRLNDLLPKATKD"
     gene            complement(20179..20718)
                     /locus_tag="VMZ53_23535"
     CDS             complement(20179..20718)
                     /locus_tag="VMZ53_23535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31506.1"
                     /translation="MARCAICYTVVGATEASQQCPDCKTDYHQSCWNEIGGCGTYGCK
                     KAAVAEKPPVPVLVGQGWGDQKPCPNCNAQIGSSLLVCSCGAKFPWADPMTNKEYREW
                     SDKQSRINTARKALIWMFIFSLVGFVAPVVGPLAGVYAWFKRKDLAASGGTYLAMGYG
                     SAAIGAMYLAIIVALKAGG"
     gene            complement(20721..21128)
                     /locus_tag="VMZ53_23540"
     CDS             complement(20721..21128)
                     /locus_tag="VMZ53_23540"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HUS31507.1"
                     /translation="MSLRGRTLDVALGGLAAAQLGIAAWLRPDGNSVSLADGRPLGGM
                     CLVHNLFGVDCPFCGMTRSFVALAHGEVGKAFEFHPAGPLLFLAMIVFVLSVGYVTVQ
                     RVTPLVERKRFLFTFQSIALVCLAIGVFKLVRS"
     gene            complement(21128..21766)
                     /locus_tag="VMZ53_23545"
     CDS             complement(21128..21766)
                     /locus_tag="VMZ53_23545"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HUS31508.1"
                     /translation="MEAVRKFKANPKMEGHPCKWCQIPLRLGDDAAVCNSCQGEHHGQ
                     CWDNKGGCATQGCVNAPLPQMQQQQQPMYGGQPAYGAQPGYGAAPQVSQQAALAAQGL
                     MSCPRCGSAIPIGSQICMNCKHITSPDGIYHGPKTNAPGASQSLVFGIIGLFICGIIF
                     GPLAISKANAAKAAMKMDPTLGGEGLATAGMVLGVLDILGWAVVLMLRVGGS"
     gene            complement(21790..23154)
                     /locus_tag="VMZ53_23550"
     CDS             complement(21790..23154)
                     /locus_tag="VMZ53_23550"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS31509.1"
                     /translation="MSEQQDEHIRIQAHELFDPEVDKGLRQQKQGRERIVADPPPVSP
                     IKRLLLNSLFYLPFASVLGALACWMMLEPKIDDFPVVGGEVQLINSDPFDTGPGMKSL
                     TIGNNEVYVDPDKTLFEVGAQGQPAFTSFDQIQVGTKVDATGLEQGSRLIAASIRPTD
                     DPTARGVVDKPMWPLFVLFPLTAALIALGLLLAEGLTTRNWIRMIERSLLGSFMAALF
                     ATLAFLPAGLILSISQSTLQDELAHGDKLIVTIKDVSAWHFFVFAACRSAAWACIGCA
                     TGLGMNIIRSTKTQLRNSVIGGALGGALGGLFFDPIDRWWKASMFEGGDMSRLVGLVA
                     VGLSIGIFVALVERLGRDAWLRVRTGPLAGKSFILYKTPTILGSSPQSDVYLFKDAEI
                     DASHAQVHRVGTVYEIEDMSSRMGTSVGGNKVRRRRLVSGDQIIIGSTILDFEERAKR
                     TPVG"
     gene            complement(23151..24527)
                     /locus_tag="VMZ53_23555"
     CDS             complement(23151..24527)
                     /locus_tag="VMZ53_23555"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HUS31510.1"
                     /translation="MSSLDIRAIDESDLKKEKAPAVKMEYRVFLSEEAFDRAVERGTK
                     DTSREIGGVLVGNVYKDDQGPYLKIETTIDALHADEKGAELTFTHDTWDHINKEMDGK
                     YKDKKVVGWYHTHPGFSVFLSDRDQFIQKSFFNLPFQVAFVYDPKSKEHGMFTWHDNE
                     IRRSRRYWIGAKEQTWDGNRASGAPEKKDVERKDDESKSSGGGSSEPLLGEGPGAFAA
                     IVIIGLILLLLGGFIGRWWGMKNTVEIAQQAQIEIVKAKAEGQASTLASLQGELVGVL
                     RSVMGDEALHKPVEDAVTSLDALLKDLDAPAQPGTDQAKKLKETVITLRNQLATLAND
                     RSSAQATLEILEMASKSTSLIRADLAREVAQQRAAVGLLYSELAADIAKNGDKARATR
                     LLQTAARLDPGNTSQYQMQLKSIDSSATLPRANDVAPQPEAPKGPTPPAAGAGSGATA
                     SAGSGGVQ"
     gene            complement(24524..25726)
                     /locus_tag="VMZ53_23560"
     CDS             complement(24524..25726)
                     /locus_tag="VMZ53_23560"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HUS31511.1"
                     /translation="MESVDLREDRYHRQSLIQWWDQQRVRKTRILVIGAGALGNEILK
                     LLALIGSGYTLVYDPDHIERSNLSRTVLFRESDEGQGKAEVAVRRMREINADVKAHAY
                     TENILVKAGLGVFHWADVIIGAVDNREARVFMNSCAARVGKKWVDGAIEALSGVVRVF
                     DPKNGPCYECTMNSTDRKLLAERRSCAMLAREAVTRGHVPTSAVAASIIGSMEVQEAI
                     KVVHGQPTLDGEALHLDGLFAEASRIKYPRRTECAGHENLGEIIPIGSGSETKLGDLL
                     ARAERELGEGASLDFSRDIITTLTCPECGDTEPGRAVLGAVRESQAACPKCKAHRVVE
                     IASSVSRGDSLDLSLTLGELGLPPFDIVVARQGMDRQQAWLLDGDAERVLGPLAESWT
                     PSGGGKLQ"
     gene            complement(25713..25991)
                     /locus_tag="VMZ53_23565"
     CDS             complement(25713..25991)
                     /locus_tag="VMZ53_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31512.1"
                     /translation="MGDINLEVWDATGNKRLKVTVPNDVAVERILMVLADKLHLPRYA
                     PDGQLMSYKFHHRRLGVQLLDDQTLAQQQCVEDDIVRIQPEITAGGER"
     gene            complement(26014..26679)
                     /locus_tag="VMZ53_23570"
     CDS             complement(26014..26679)
                     /locus_tag="VMZ53_23570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31513.1"
                     /translation="MDADVGHPADAKIYLDGRIEPQPDAQIVHPPADASIDAPKPVDA
                     YVCTVHTKQLLLNPALDLAPAGTSWVMQNIDNAFPVVTDDPPLAPHSAPYKAWMGGIS
                     GDDIGASSATDGLYQDVQVPAGTTSLVFTGFYDVRTSETGTTVYDRGTVSLTKTDGTP
                     LETIASLSNAAPTAAWTAVSKTFSGNYSSQTVRLKFTTTNDVINATSFYFDTFALTAT
                     YCQ"
     gene            complement(26901..28304)
                     /locus_tag="VMZ53_23575"
     CDS             complement(26901..28304)
                     /locus_tag="VMZ53_23575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HUS31514.1"
                     /translation="MAAAALTTPWGCPVLASSVSQSSLSWSDHPGRALLKLAWPITVS
                     MVSFSTMTLASTAFVAHVGADELAGVGLAGVVGFTLVCFGIGLLRGAKTLVSQAVGAG
                     RRERIPELLGAAVVLAVALGILATITAHFVAPLLVQLSASPRAGHFAAQYLAIRSLGA
                     TLVLVYAALREASYGVGDSRTPMRASLAGNAINIALDAVLILGLHWGVKGAAVATIAG
                     NVTELAVLAIPMRLQLAKLKWSVSAARAVWAQGVPNGLQFIMEVGAFLILTVLVARMS
                     AVDGAAHQMVLHLVNVSFLPAHAMAEAAAVLVGQAVGANRDVLVARVAKRALAIGAGY
                     ATVCCVAYAILGAHIAQAMSAGDDVLAARATMLVHVSLAFLVADAANVIARGVLRGAS
                     DVRYAAIVGIITSWLTTPPLTWWLGVHLGLGVVGGWIGLAIEIVLGASLFWLRVYRGG
                     WKPAAAAARRAMAGTAV"
     gene            complement(28603..29529)
                     /locus_tag="VMZ53_23580"
     CDS             complement(28603..29529)
                     /locus_tag="VMZ53_23580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31515.1"
                     /translation="MTTVGTKCIVKLGTRWTPSTIVRCNPDQTFDIVPIGEEDDFMAK
                     WHGVTRSEILIDDAAAWPDVFDSLRGGAAGIDSAAARAAFGKLGATFEDESWHAYWDR
                     EAKAEVLDRERAYEFFVGSGAPALLFANPSLDPSRDLFKLYWNQVRMGGRDPSDVDRA
                     ITIVDTVRVLGLAEAAEDPEQHAAIARFATRHRIKLPATLETLWGKADVLQKIEDSHC
                     NNPEPQGADDWQLRDHEGRHAARIMLPHQGDHAWWAVFADGAPDAEIWVSFEEDGGPV
                     RRVAHSLAFFFWDLAKTGRSWDLSQAAEDAED"
     gene            29641..30621
                     /locus_tag="VMZ53_23585"
     CDS             29641..30621
                     /locus_tag="VMZ53_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUS31516.1"
                     /translation="MSSISEDVSKLQGRFTALKAEVGKVLVGQDEMLHRLLLGLLAGG
                     HVLLEGVPGLAKTLVVRTLADALDGTFSRIQFTPDMLPGDVVGTQIYNPREGTYTVKK
                     GPVFGNFILADEINRAPAKVQSALLEAMQEKQVTLGDQTFKLTEPFLVLATQNPIEQE
                     GTYPLPEAQLDRFMLKVRVGYPTRDDELKIIDRMAGSGSEPSASKVMTCDEILSARDV
                     VRRVFVDDKVKRYAVDVVAATRDPKASGMPALAPLIDNGASPRASIALVKVAKAQAVL
                     SGRDYISPHDVKTIAHDVLRHRILASYEAEAQGKTTDHVIAQILENVPVP"
     gene            30631..31608
                     /locus_tag="VMZ53_23590"
     CDS             30631..31608
                     /locus_tag="VMZ53_23590"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUS31517.1"
                     /translation="MLPAEIAQAVKRIQFVTGRQVADVMAGAYLSVFKGRGMEFDEVR
                     PYVPGDDVRSIDWNVTARTGEPHIKRFQEERELTVMLLVDVSASQDFGSGRRSKLEAA
                     VELSALLALSAVENGDKVGLLAFHSGADLYIPPRKGGKHALRIVREVLARGEGAAAGP
                     QRLPMLPVFVRRWFAKLQKTTRAQPSPRRSTSIAAALEFCRQVLPRRAVLFLISDFLD
                     EGYLQVLRHANRKHDVVAALITDPREAEMPPAGLVTLEDAETGATRLVDTRSAQFRSE
                     LAAGAKKRQKDLEDQLRATGMDLVVFDASQSMVDPLLRFFKQRERRLRR"
     gene            31605..32645
                     /locus_tag="VMZ53_23595"
     CDS             31605..32645
                     /locus_tag="VMZ53_23595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31518.1"
                     /translation="MRWLAVLLLVGACSNRSALDSPDQASKLEPSKDAITKTSENGPV
                     KATVSVWPAKPTLGESIYLRLDIDAPAGISVDAPFQEAGDQRLGRFKVVGFVKDAKRG
                     ANGAQHQEQTYTLEAPASGKHRIPPLRLEMVDSRSDAKAAAQEILTEEIPLDVAPVPT
                     EAIGKELHAAAGKLDPDVGGTPWLWIGLGASILAVAFAGSLLTWRYFRVRRSLEEKKS
                     AYEEAITKLQSLEARGAPAAEQADAWFVELSAVVRDYVEGRYGLRAPELTTEEFLYIA
                     ARWPAMSALDRTQLSSFLERCDRVKFAGYRPESQESMELLALARKFVEDTRPRDEAPA
                     TVSADGLAKGAA"
     gene            32650..33687
                     /locus_tag="VMZ53_23600"
     CDS             32650..33687
                     /locus_tag="VMZ53_23600"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS31519.1"
                     /translation="MSWQIPGTNVELAHPALFLFALLAIPAFWWSRRGAGRVVFSSLR
                     ALPWGGQTWRTALAWVPDALFALAFVALAIALTGPRSGDKSSRIRREGIAIVMAVDVS
                     GSMRALDLSEKNQELTRLDAVKRVFEQFVLGGKRLGGRPDDAIGLVAFARYADTRSPL
                     TLDHANLVNAARQLDFATQDEDGTAIGAGLELAVQRLAEYKARSRIVVLLTDGESNVH
                     DIDEDTAIEDAIKAGVKVYTIGAGTNGVAPIAVDRGDGRKQLMQMTVSIDEKTLGKIA
                     EKTGGQYFRATDSASLRRIYQQIDKLERTTIEENRFLEYNQYYGIFVAAAGLMIAAAL
                     ALRSTFLRRLP"
     gene            33684..34712
                     /locus_tag="VMZ53_23605"
     CDS             33684..34712
                     /locus_tag="VMZ53_23605"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS31520.1"
                     /translation="MNLTWQHLERVHLVWVVLLFVACLVVLEIKSRSTLSSFLSPVMQ
                     RRLTAQPSFTRAMVKLALITLAMLACVAALMRPQARGETETVTVSQASADVLFALDVS
                     RSMLAEDAAPNRLARAKAEIGQLVAKLEGHRVGLLAFAGRAVPVCPLTPDHSYFNTVL
                     GTVDTRSAGKGGSRLAEAIKAAVRAFPQGQGAKLMVLITDGDDQDKPEYTQEAAKAAR
                     DAGIKIVVVGIGSEEGSQITLTDPATGAKTTLMHDGKPVISKLDGGALRKIALTTEGA
                     YIPAGTSAIDLDSIMESHVTPIVRQAAAGSVRVVPAERYVWFVLAAVVLLVAALAVGG
                     SGLERRTR"
     gene            34709..36976
                     /locus_tag="VMZ53_23610"
     CDS             34709..36976
                     /locus_tag="VMZ53_23610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31521.1"
                     /translation="MSTIVRTLVVAILVMCACAKSEHREAREKYNQGVELLVAGDHEG
                     AEKALLDARSTAGVDPELRFRAAYDLGMAYAAHAEKLKTGKEPDLNKALELTEQAVSW
                     FSDAARLRKDDADTKANLAIVRARAQSLADELRKSEGKLEARLDAVIADQRSVLDEAR
                     GAWIAIKEAGGTDPLAQQGTLTHLADKERGIVAEAGVIGDLADSEIDEIAKKTEDKRT
                     DEEKVRLVVLKNLDVYLAEARGKIAEARGKLQNLAAEDGVDRAEAALIALKRAREQLL
                     DPITVLREVAQDELQLLQETSAVADVEAGKLQQLKPGETPVIPAWMSGPALALRQLGM
                     HDRVEEVRARLAAATEGPPPAPPQGADPAQVEQQQKLLERVKVALPLVVDASTAMDKA
                     HGKLIDKKMQEAVLAERDALVALAKAIEQFADLKQTIDIASETQKQIVGALTEKKNAT
                     ETKEGIAANLARMPRIKELLADEVAKLEKQAQQPADPKADPKQLEAQKQQIDQMKQQM
                     ADAEKLRGEAEKLVADTDAAVKANKDPLTPAKAADAKLDELRRIFFSVIEHLQDLIRQ
                     QGESRDQTSTAHTQTDEERAPKLPGLASRQDQHGQLAKAITDALAAQADAAAKGQDPN
                     AQQQAKSMSAAADEVRLAQNDMADAKRTLDKAASPQTQQSVSLEPVLKAQASALEHLM
                     NALKLLQPPQQQKQDDKQQQQQQQQQQQQQQQKQQQQPQQGGAGQRARDEDARRQKER
                     REREQSQGDAVEQDW"
     gene            37171..39804
                     /locus_tag="VMZ53_23615"
     CDS             37171..39804
                     /locus_tag="VMZ53_23615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31522.1"
                     /translation="MLVLLLVLGAARGAFAQTARISLDETPHVGMPFTLGLVAEGFDE
                     APAPAQPKLEIPGAVVTPMGAQPNVSQSIQIINGKRSDSKRVTWVFRWRVEINKAGRA
                     SVPALTVVQGSKKATAQGGQFEVESVPTTDDMKLEVALPNRTIFVGETIEAKLTWLFR
                     VQPQQDPQFSVPLMAMDDFIVSAPPATDPRRALSFAAGTKELQLPYEVDETDVGGQQY
                     HRVVVRFYVAPKKAGKNDIPGASVTAALPVGRPDFFGNAPSRMFRAADVARSLDVKPL
                     PETDKPPGFAGAVGSQYSIAVGASRSVVQLGEPVELTVTIKSDQRLDTLSLGRLDGPG
                     GLPKDKFTVPADAPTGELSDDGKSKTFKVTAQVIAPASEIPAIAFSYFDPVKSRYQTI
                     HSDPIAVSVKGGSVVGANDVVAMNTTKPGEKPRAITTPETDVALVGADLALSAPGATH
                     DKPMGGSLLWLLVAALYLVPLALLGLRVYQLRTQTEREEASEVRAARKRVEQELARAA
                     KEPARDTAGALVSALRAFARAVERSPDDDGGLLAKIETESFAPSASSSPLSSDLRTRV
                     EDLIRRWTAEANRKRTSTGTTTAAVTIIVAALAAPHGAARAESTAPSPAAPSTLATSS
                     TLVADASGAATPANTATMSGDALANGRSAYQQAMTLSDASARKAAFQRAQVALGEAAH
                     AHPDSPELLADWGNAALGAGDLGTATLAYRRALAIDAENARARRNLEWLRSKESDTFR
                     PHGSVAATDALFFFHTWARGRRVLVGAFAFAIGVLLLVPWGAKRRRWMHALAIVPGIV
                     WLALTISVLVEDRHDEDAVVMEGVMLRAADSPGAPTTLAQALPRGAEVAVVEQRDGWT
                     KIRLASGTAGWVPSSAVERIH"
     gene            39840..41000
                     /locus_tag="VMZ53_23620"
     CDS             39840..41000
                     /locus_tag="VMZ53_23620"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HUS31523.1"
                     /translation="MTRRLYHEDAYTRRFDAQVIAHATYKGEPALVLDQTAFYPEAGG
                     QLGDRGVLGGLAVRDTQELDDGTIVHLVGGELPAIGSRVEGELDWARRRQHMAQHTAQ
                     HLLSGSLLDRAQAPTASARLGESALTIDVTRDRVPEPELAAAEDLANDLIDDDLAIRA
                     WFPTPDELASMKLRRDPKVSADIRVIAIGEFDFSPCGGTHCSRTSQLGALRIIGVERY
                     KGMTRVTFTAGRRARAELFNRDQVLRGLATQFSCGPAEVPAAIDKLKRDVESSSAELT
                     QLRSRLASAIIDAIPGTGPAVASIPGDAELLRSVAAKLVSAGRDAILCAPEPGGATVV
                     LFRAPGSTLDCGALWKKVVAKAPGRGGGRAERAEGRLTVEIPNWAEFIDDLA"
     gene            41142..45176
                     /locus_tag="VMZ53_23625"
     CDS             41142..45176
                     /locus_tag="VMZ53_23625"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HUS31524.1"
                     /translation="MSRIVGIGASAGGLESLEKLFSAMPADTGMSFVVIQHLSPDFRS
                     LMDELIARHSDMPVVVAEDGLPVQPNHIYLLPPRKEMIIRNKTLILTDKPNGLSLPID
                     LFFRSLAQDVGAESVAVVLSGSGSDGSRGVIDVKRAGGLVMIETPATAKFDGMPMSSH
                     ATGVVDHSATPRDLARLLCGLPPLDPVDEMEGDQVLNEDPAMDSLLRQLRDGFGIDFS
                     LYKITTVARRVQRRAELMRAQSLDQYAEHVRNDPSELNTLYQDLLIGVTQFFRDPESF
                     DLLEREVIPQLLDKIPPEQEVRVWSAGCATGEEPYSLAMLFYEQMSARQRPLNLKILA
                     TDVHQLSLETASAGIYGEQQLANLSPQRRERFFIKRATGFQVSPELRSLIVFARHNVT
                     KDAPFTKMNFISCRNMLIYFQPQAQRTVMSLFHFALAPNGVLFLGASESPGPLSDEFI
                     SVDEHWKIFRKRRDVALLSNVRLPTLGSPIRRQPFEPPRPHGPDPLILATYDQLLDRF
                     MPPSLLVDEDRNLIDSFGGAEKLLHVRKRRPTSNVLDLIDGELKNVIGGAMQRAVKEN
                     GRVAFSGVRMLESDGGEKRCTVTAEPFVHPRTGARSILVAFLDVTPIEPFVEAEAPVA
                     ASTMQHDQASRDRMETLETELAYTRETLQATIEELETSNEEMQATNEELVASNEELQS
                     TNEELHSVNEELYTVNAEYQQKIIELKELNADMQHLLEGTDVGTVFLDRELRIRRFTE
                     RIASVFRIQPDDVGRKIGDFSHHLVRPTLMQEIEKVRARGVTIEEEVRDRDGTPYFLR
                     ILPYRVTRAGDAPRKLESGADGVVLTLTDISTLAAARSRLAQLSAMVESSEDAIIGKA
                     LDGTIIYWNRGAEKLYGYTAAEAVGKHARMLLPPEKTDETDRFISTVRKGGKVEQVQT
                     IHVRKDGTRLEISKTVSPVFNSEGDLVAVSSIARDISALVAAQRAAEDGQERVRALLE
                     STAEAARRREQFLAMLSHELRNPLAAVLGATSVLAANPERATSERCCNVIERQARHMA
                     RLLDDLLDVSRITRGKFELRSAPLDLRAAIDGAIESTAPLFEQKGVTLDLALPDEPIP
                     MTGDIARLQQVIVNLLSNAANYSARGNRVELRVMPQGDQVLLRVLDRGFGIEREMLSK
                     IFELFVQAEQRIDRPRGGLGVGLSLAKSIVELHGGSIEARSDGPMMGSEFLVRLPLTK
                     PAETRASKPHIQRGPRRRIVLVEDQNDAREMLRMLLESLDHEVLDASDGASGVELIEK
                     VKPDFALIDIGLPTMTGYEVAQRIRQSADLQSIMLVALTGYGTANDVVAAHDAGFDAH
                     LVKPADLVRLQQLLASHKKDDSFD"
     gene            complement(45180..47063)
                     /locus_tag="VMZ53_23630"
     CDS             complement(45180..47063)
                     /locus_tag="VMZ53_23630"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31525.1"
                     /translation="MPNASAAATGGQSRLGRYELLARLATGGMGEIFLARLEGAEGFE
                     KLYVVKRILAHLADDTRFRQMLIAEARIASKMSHPNVCQVFELGDSDGQLYIVMEYLE
                     GVSLLPLLRRFSKNQQQLELGFIAGVMQQTTDAMNYAHELKDRDGQSLGIIHRDVTPS
                     NIFVTESGVAKVLDFGIAKARGASTQTQEGTVKGKYAYMAPEQLKGSGIDRRVDVFAM
                     GVVLYEMLALRRLFQRKTDYLTFRAVMEQPIPDIRRYRPDLPDGVAAALTRALERDPN
                     DRFETARQFGSAVLDGMAQYSRPWTQGEMSDFVAHYFADEIGKRHQQVSAVINRGVPA
                     RSTMPLFSMPDEETQVDDGDDEMFPSVETEVEGAPPWMPRPQSPAPNPMGPQNSQEFA
                     GHSSSGTPPPFGIEPTGSAPMLQPLRGAPVMPPTANTPPIVMVSQRSFVWPVVAILMV
                     LIAGGALFLVWKQMQNQQPPAEIKITQEGGGGGDKPIQTTGSGDTTTYNMDETNPTKG
                     SAAETPHETGSATPDTKPGPGPGPNLVRDKRPKTYDEAVQQKLADLNACVKAHPESLP
                     KDAMSAKITVAAAGHAQTVVIEPEAVNATPLGACLRNVLMKARYPTAGSDLKVTVPLK
                     QKS"
     gene            47168..48001
                     /locus_tag="VMZ53_23635"
     CDS             47168..48001
                     /locus_tag="VMZ53_23635"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HUS31526.1"
                     /translation="MRFELHCHSNCSDGTETPDAVAARAIARNVEIFALTDHDTCAGT
                     VATRTGQSATIRTLRAVEISCDDPFTGRTLHVLAYDRGHAGWADLELKLADLREARRN
                     RMRVMAAKLAQRGIRIDVEPLIADAEASGRTLGRPDLARLMIAHGAVSSMKEAFSRHL
                     YDGGPVDAPHRALTLADALALGRSANAAMSLAHPHLYDHLGVRLLREFAKEGLVGVEA
                     YYAAYAPAERNRWLDLAKELGLVATAGSDFHSPDDAVAPVGVDVPDDHGKRLRDWLLD
                     A"
     gene            complement(47998..48393)
                     /locus_tag="VMZ53_23640"
     CDS             complement(47998..48393)
                     /locus_tag="VMZ53_23640"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31527.1"
                     /translation="MTVEELIAKAYEHDREGREKEAVKFYDEAWRAGVPEPQRKRFLV
                     GYASTLRNVGRAEEAVAILGEAVAAYPDYPALVAFLALALADAGHPKAALATMLGCAL
                     DVAKPAIFDGYERALSEYHRLLLDEAATT"
     gene            complement(48390..48944)
                     /locus_tag="VMZ53_23645"
     CDS             complement(48390..48944)
                     /locus_tag="VMZ53_23645"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HUS31528.1"
                     /translation="MKTEPETFSFDDLERVRIEPWTGVRSMFARFHMRNMNIDDEVLF
                     YHSSCEPPGVAGLARITRIRLVDETQFDPQSKYFDERATREKPIWDCAEVQFVRRLPR
                     FVSLWEIRAEPSLADMPLLKKGMRLSVQPVTEAQYRRIVAMANEPAPPEPAPKPKAPA
                     KKKPAKKKPVKKPAKKPAKQKKKR"
     gene            complement(48944..49876)
                     /locus_tag="VMZ53_23650"
     CDS             complement(48944..49876)
                     /locus_tag="VMZ53_23650"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS31529.1"
                     /translation="MLGPLRHVGAGLAYFATASRQQKSDSLTARQHATPLPLPKGLEL
                     TWLGTAGFRFAYQGTVLWIDPYVTRLPLRDILLRRVVPPSSDAVARWVDKADAVLVGH
                     THFDHALDVPAIAKHTGCTVYGSQSLQNLMGLYGLAPQATVVEAHRDYEVGPFRFHFV
                     PSVHSKLQLGLGIPYSGELTCEHLDELTPQAYKCGQVWGIHLEVAGMRFYHQGSADLL
                     EAEIRDKNVDVFLCGISGRRFTPKYVSRIVRALAPQMIVPTHYDNFFRPLDAATRFSF
                     NVDLTGFADEVHAASHDVPLHVLDLGVPCRSSTG"
     gene            49924..50517
                     /locus_tag="VMZ53_23655"
     CDS             49924..50517
                     /locus_tag="VMZ53_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HUS31530.1"
                     /translation="MAFTLPPLPYGKDALSPVISPETIDYHYGKHHQAYVNNLNNLVP
                     GTKYENMSLEEVVKATNGQASEKKVFNNAAQVWNHTFYWHCLAPKAGGDPTGNIQAAI
                     DKSFGNFADFKKKFNDAAAAQFGSGWAWLVKSGDGSLAIETTSNAETPFAAGKTCVLT
                     CDVWEHAYYIDYRNARPKYLEEFWKLVNWEFANKNFG"
     gene            complement(50514..50966)
                     /locus_tag="VMZ53_23660"
     CDS             complement(50514..50966)
                     /locus_tag="VMZ53_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31531.1"
                     /translation="MSYLHCPTCKRAYNVAISTQCPSCPVPVQLVDAAEDIVAAAEAL
                     ARAMQRATPDERDDAMGRMDRLALPAPGAKPVTFQGAMLRSIREALDPPPAAHKPQPL
                     VATIARALIERVVDHPRAIEMRERLARRAPRLREGWRRVRALASAAAN"
     gene            51089..51886
                     /locus_tag="VMZ53_23665"
     CDS             51089..51886
                     /locus_tag="VMZ53_23665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31532.1"
                     /translation="MRLVVLTLALVACHPGQTPREPLPLVCSEDRVGNVIVEGGTRAD
                     VPELAVLEGTLDDAERTDRIVQVSKDLLHARGYSRAEIEVERRAGCGVELVVHVDRGP
                     RFRVAKIAFDTDDEFPAKARYAAIEDALGTVNAVGGSLVVDRLGRALEGLKRQYQDLG
                     WLDVEIEHADAVYDEASGKVSLTIPIHAGQRYKLGNVVARGGRRSSRAAVIDALGLRG
                     GDWYNGRAVREAIRRARRETGERIEVRLEVSEGAAAIDLEAVLRGVK"
     gene            51883..53202
                     /locus_tag="VMZ53_23670"
     CDS             51883..53202
                     /locus_tag="VMZ53_23670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HUS31533.1"
                     /translation="MKPSLFDWLTLAFAVSLAALCIVLAGCSANAHYLGAPLPAACSK
                     GDVERCAGWMAERDLVAGQLDVYDDPDLRAYVQRIADRLAQGAGLERGPRIVIANHDG
                     TYAAYGERIVVGRMAIEKLGSEAELAAIVAHELAHVEGHHASMTLFGPDADAVWLAAR
                     RDAEAIADERAVALLERAGYTPSAMRRALAASLDADDDEHPPKTDRLTAVSTLAAGRT
                     DGFDGRDELLAHVDGMVVGRDPQLGMRIDNAWVIALLGVAIQLPERDVVRVDGDELVL
                     RRGRSALTAYPIGAAWARELAASLDERASVDTRLGPITVGVTPSPAETATPIGKLQSA
                     VRELLPQPAPGTWVVILVRPGGGLVLELAAKSDPFLRDRWLSGLRAASPSELLATEPT
                     RIAIHRAPRAGRVAELVEECPDPYRALALEDPDRMLAEGQPFKCTDR"
     gene            complement(53216..53689)
                     /locus_tag="VMZ53_23675"
     CDS             complement(53216..53689)
                     /locus_tag="VMZ53_23675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31534.1"
                     /translation="MTREFLDTIAPYREILVALTSMLVALWIAQAIHGWRGSWRARRR
                     AERAGAGEDAAADMLEEAGYRVVEKQARVLWAPLLDGEPQLLELRADYLVEGDGQLLV
                     AEVKTGELAPSIETAATRRQLLEYHVAFAVDGVLLVSPEQGTIQRVAFPRVMPCA"
     gene            53785..54297
                     /locus_tag="VMZ53_23680"
     CDS             53785..54297
                     /locus_tag="VMZ53_23680"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HUS31535.1"
                     /translation="MPRIEVIQGDITKLDVDAIVNAANSSLLGGGGVDGAIHRAAGPD
                     LLEQCIRIRNAEGGCKTGDAKVTSGGRLKAKWVIHTVGPVWDGGTEDEPELLASCYRN
                     SLQHAAELGAKTIAFPAISTGIYGYPLREACEIAVNTISAHAGDAPEKVILCMFSKKD
                     YDTMLEVVEG"
     gene            54300..54812
                     /locus_tag="VMZ53_23685"
     CDS             54300..54812
                     /locus_tag="VMZ53_23685"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HUS31536.1"
                     /translation="MPKIEWMQADITKLRVDVVVNAANETLLGGGGVDGAIHRAAGPQ
                     LYDECLHLDGCETGEAKVTKGYRLPAKHIIHTVGPVWEEGAQNEPALLASCYRKSLLL
                     AQSLGARSIAFPAISCGVYGYPIRDAQEIAVKTINAHAGPLPELVILCTFDDETTEIT
                     REVVASVLAY"
     gene            complement(54809..56029)
                     /locus_tag="VMZ53_23690"
     CDS             complement(54809..56029)
                     /locus_tag="VMZ53_23690"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUS31537.1"
                     /translation="MSEPLERRLRIEIAPKTIALVFAIVAGLWLVAQLAAVIEVIVVA
                     LVLVGTFDPIVAWLEKRGLRRGRALVLVFVVAGLAVAGLVLLMVPPLVAQLVDLISNA
                     PKVRDDVIKSLQGRHGAEPIIKSLQELPVQDLGARAATILVGYSTKLLLLIGYAISTL
                     FLAMYFLADPTRSKGLIYSVVPRPHHVKTAKILIELRAIVGGYMRGQLITSLAISVFV
                     FVLLTILRVENALAIALFAGLTDIIPFVGGYIASTPVVLAATQRGTTATIIVIVLMIV
                     YQEFESRILVPSVYGRVLRLPPAIVLIALLVGGTLGGIVGALLALPIAAGLQMLAREL
                     RVDLPGETPASDATLHTDEKASKVYEQLTEDLPAKDAGVIADDLATIVKKAEADDASV
                     SDRLPIAPPDPKPA"
     gene            complement(56100..57302)
                     /locus_tag="VMZ53_23695"
     CDS             complement(56100..57302)
                     /locus_tag="VMZ53_23695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31538.1"
                     /translation="MKYGGLLVATLAACAPATSTDEAAITTAVRHERFGLIRDSAAEM
                     GLYNASLLAGVAISETNLAHCQSEATFACMGPASPSCNGGPVIAGAADGPCSAMQGGL
                     GMFQFDSGTYQDTINTYGDSILTIEGNTAQAVNFVEVRAIQDKHATDWLSAMDWLNNV
                     QMQTGTMTLEEWADFIVCRYNGCCTTSATCSSRRSGYRDNALKAFDEMGAEFWDTSGR
                     CAGVPAGGVIDQRSECYIAGGDPRYWRREPGGYGANGREWTGTTSAAAPANFGMWIIK
                     VPAGRYPVDVYLDGGEVGTSVSATYKIVHAGVTDTVTVDQTSVSGWVNLGDFDFAGAG
                     DEHVLLGDNTGEAGSMDRHVMFDALRVPIDDSEVADGGCCGTGRGTGATSLVGVGLFA
                     LALRRRRR"
     gene            complement(57344..58138)
                     /locus_tag="VMZ53_23700"
     CDS             complement(57344..58138)
                     /locus_tag="VMZ53_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31539.1"
                     /translation="MRALLIVFLLGCDMGVVAEPTTPEPAQPAFLLPSLYPRSPYQHV
                     HDFQEYALRDGRVVRYDAYNANVVSIFEPSTKQVTELGRAALLAVPRMGWLVLTRGAD
                     GTVRLNDIDPSRDALRLVWQGGYPEVVGTLDGAIVISSERELIALTAPGVAQTLAKLP
                     AGMSPASGDAIRGHRLLLVSGGAKEDGSWPARMTFDADVSIMDLQTSRVRKVGIAKGG
                     YTMQTAIPHPFVLVHWADHEQLASERSWAEGRPNIVDVTTEQISHR"
     gene            complement(58207..59013)
                     /locus_tag="VMZ53_23705"
     CDS             complement(58207..59013)
                     /locus_tag="VMZ53_23705"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HUS31540.1"
                     /translation="MKSAVVVTLALVGSLRPTLAHADRKHPLYVRGGVVHLEPLEQSR
                     PMELADVDGPASLAVQNGPIEGSGATVSSATIPGIIVGYQLPWKDGRIAIETIIGLPF
                     TVKFRATGTLANESIAPMALGLPTGVGPLGSELGEAKAVPPVVTMIYTFGNSPRIHPY
                     AGGGVAVMFSTDAKVTNPMLTAVSQPKMSISPAPGVVLQTGIDAKITNRVYARLDVKF
                     IAGMLARARVEHIQVQTPELPIFDTVEVGTAKMSMWVNPLIVQLGVGTDF"
     gene            complement(59010..>60071)
                     /locus_tag="VMZ53_23710"
     CDS             complement(59010..>60071)
                     /locus_tag="VMZ53_23710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31541.1"
                     /translation="EPVRRTSVTRGMACNQGTFRVEETDAAGTCTAVVAGTLIARERT
                     MTFIPDVPWVMGKRYKLTLVSGTNDSCDAGELCDPSSALSFDPLAGLGGGDGGGPALT
                     MPFTGGAPSKGTYMMNTGAPATDVNGNGVVDSSEQMRTENRAAMHITSTTGSINSATF
                     NGNTCGDAAVDNKKGCTYLVGAMPVVMGEATTTCPLPGGATAATCIPVQMSAQTMFGT
                     SITMAADIGLTTISTATGTQIMRIRQGTGGQVMGYIIDNGGTPKIMVALDLYMDAPDM
                     SVTLSSHDLHSKQLSLVLEGPVTFLDDGRISIALSNTADVPVDVSISGPASGHVKMSI
                     PKGEMNLQLVSPAPRGVSL"
BASE COUNT        10420 a        19759 c        19836 g        10056 t
ORIGIN      
        1 cggcccgcac gtcgtcgatc actttggaca gctcggcatt ggcttcggtg agttttgcgt
       61 cggcggcgag gaggcgcgca cgcgcgaggt gcgcgtagtg gtgcggcatc ggttggaggc
      121 gacggtcgac gcgggcggcg agagcttcga gatcggcggc gagagtgtgg gcttccgcgg
      181 cgcggccgag gtcgacgagc gatgtgatgc ggaccgcgag cgcgagaagc gcgtggtgct
      241 cgcgtgaggg tttggcttcg gtggcggcgg cgagggcgtc ggtaggctcg ttgcgaagct
      301 cgtgggcgag ggctttgtag atgaggaagg gcgcgcgcga tgcgtcgctg ctgcgtcggc
      361 ggcggcgcag ctgcttggtc gagatggcga ggaggctgtc ggcgtcaccg ccggagagtg
      421 cgcgcgagat gcccgcggat tcggcggcgg tgcggaacac gagccagagc acgatcacga
      481 agccgaagcc cagcgccatg aacgatttgc cgaaggcgcc cgtgtcactt gtgagcgcga
      541 ggatcgtgta ggcgcccgcc atcagcgttg gcgggagcag ccaactcgca agccacacga
      601 tgcgtgccca gagcttgccc gcgcgcgttt cgggctcggt aggcgccggc tgttcgctgc
      661 ctgcagcagc tgggtctggc gtccagtccg tcgtgggagc agtgcgcggc acgacagcgg
      721 ctcgctcgct aggtgccact gcggaggtgg cgcgagatgc gaagcgcggt ctcgagcgct
      781 tgctcgtaat tgagccgcgg atcgacgcgc gatttgtacg cgtgggctag gtcggcttcg
      841 acgaggccgc gtgcaccgcc cacgcattcc gtcacgttct cgccggtgag ctcgatgtgg
      901 acgccgccga gacgcgagcc aagccgcgtg tggagctcga acgattgctc gagctcgttg
      961 acgatcttgt cgaagcgtcg cgtcttgatg ccttgcggcg tcgtctcggt gttgccgtgc
     1021 attggatcgc aaatccagag gacgcggcgg cccgtgcgtt ggacggcttc gacgagcgcc
     1081 ggcaacttct cttggatgtt gtctgcgccc atgcggtgga tgagcgtgat gcggcccggc
     1141 tcgcgctgcg gatcgagcac gtcgagcagg ccgaggagcc agccgatcgt cattgccgga
     1201 ccgaccttga tgccgagcgg attgcgaatg ccgcgatgga actcgacgtg cgcgccctcg
     1261 agctgcgcgg tgcgcatgcc gatccacggg aagtgcgttg acaagttgta gtagccgttg
     1321 cgacgcggca cgcgacgcgt ttgcgcttgc tcgtacggca gcgcgagcgc ttcatgcgac
     1381 gtgtagatcg tcacgcgacg cagcacctcg ttctcgacac ccgtgatcgc gctgacgaaa
     1441 tcgagcgact cgcgaatcga tccgacgatg cgctcgtact gcgccgcgtt cggcgagccg
     1501 gccgcgaacg agacgtccca gttctccggg tgatgcagat cggcgaatcc accgtcgacg
     1561 agcgcgcgaa tgaagttgag cgtcaacgcc gcacgctcgt atgcgcgcag catcagcgtc
     1621 gggtccggct cgcgatcctc tgctgtgaat gcatcacgat tgatgatgtc gccgcgatac
     1681 gcaggcagcg tcacctcccc gcgcgtctcg ttctccgccg agcgcggctt cgcgtattgc
     1741 ccggcgatgc ggccgatgcg gacgaccggc ttgcgggtgc cgtgcacgag cacgaggctc
     1801 atctgcagca ggatcttcag cttcgctgcg atcggatcac tgcggcagtc gtcgaagctc
     1861 tccgcgcagt caccgccttg cagcacgaac gcgtcaccgc gcgcggcctt cgcgagatcc
     1921 gccttgagat tttcgacctc ccacgacgtg acgagcggcg gcagcgcggc catctcctgc
     1981 atcacgcgcg cgagctttgc ttcgtcgccg tacgacgctt gttgcgcggc agggcgtgcg
     2041 cgccacgtct cgggcgtcca atcatctggc gagtcgtaca tcttgggcag cacgggcgag
     2101 gactggcggg cggtcgtcat cagtcgggct cctgctgctg cttctgctgc ttaccaccgc
     2161 attcgaccgg gcgctcgccc tcgtagtcga gacggcgttt cgccggcgag tacagcgtcg
     2221 ccggatcgag cttgcccttc ttcttgcgta ggacgtctgc gatcgcgtcg cgaatgatca
     2281 cgtccgtcat ttgaatcgcg ccctccggca gcttgagccg gccgatcacg ctcatgtcgc
     2341 cgcccgatgc gaggaagtcg ctcgtgacta ccttgtacga agcttcgtcg gcgatcggct
     2401 tacccgcgac cttgatctcg acgtcgagct ggccgctctt gcaccgcgcc ttcgccacga
     2461 ggccgcccca cgagtagatg cccgagccgc gctggatgtt cgtcgtgacg agccgccgca
     2521 cgtgcttgcc cttcatctcg acgatcgcga accgattgtc gaacggcatc gcttcgtaga
     2581 gccgcccata cgtgagctcg cccgccggga tatctgcgcg caagccgcca ccattggtga
     2641 gcgcgagctg cgcatcggga cgcgcggcga gcattagatc cgtgaaccaa tttccctcgg
     2701 ccgattcgag cgagtacgac ttcgtcacga tcgccgtcag ctgcacgccg agcttctcgt
     2761 cgcggcgcgg cgcggccttc gcgctcgctt cgtcggcgat cttctgcacg tcggcgtcgg
     2821 gtacgacggg cttaccctca tatgcctcgg gatgacagtc agctgcgggt ggtggattgc
     2881 cctcggaatc gagcgggcac aacagctgcg gcttgtgaat ctgcaccgcc gaaacgtgcg
     2941 tatcggtgat gcgcagatcg acgcgcccga acgcgcgccc cgacgagtac gactcgatca
     3001 ccgcgacgtc tttaatccga tgcgcgaccg tcgcgtgcgt gtgcccgccg acccaaacgt
     3061 cgacgaggcc cttgggcaga tcgctgatga ccttgaacac ttcctcgtcg gtcttgcacg
     3121 acgacaaatc gttcggcttc tcgacgtcct tgcacgcgct gccgatgtgc gcgacgacga
     3181 cgatcacctg cgcaccgcgc gcgcgcagtg cctccgcctc tttcgcgatc gcctgcgccg
     3241 tcggcgccat gacgaggccg gtgaagtttg cgggcatcgt cgtgaacggt gtcgactccg
     3301 tcgctgcgcc gatcacaccg accatcgtgc ccgcgacctc gacgaggatc gacgacggca
     3361 tgttcggcca cttgatgcga ttgcccgact tctgatcatt gatgttcgag acgaggaacg
     3421 gaaacttcgc ttcggccgca cgagccttca gcgcgccgcg cggatcttcc tcgacggact
     3481 tcgcgacgac cgatggtccc tcgggaccga agtcgaactc gtgattgccg accgccgcgg
     3541 cggtgtaacc gatcgcgttg tacgcgcgaa cgacgtcttc accctcggcg agattcgact
     3601 cgagcgtgcc ttggaacatg tcgcccgcgt cgaccagcag cacgccgccg ccatccgctt
     3661 cccgcgccgc gcgaacattg ttgatgaagc cggcgagcag cggcagccga tcgatcgcgc
     3721 cgtgcagatc gttcgtgccg ataatcgtga gcgtgcgtgc gcccttcttc gcgaccggcg
     3781 gcccgtgatc gacgtgcttc gttgcgggct ggctgcacgc agcgacgagc acgagacaca
     3841 cgaccagcga tttcatacgc gcgaccatac tatacgcctc catctccggc cggcccgacg
     3901 ccgccatcgc ccgtgacttc ttcttcgccg tcgctgtcgc cggcgccgcc ctcgagatcg
     3961 cgcgagatct tgatcagctc gcgatcgcgt tcgggcgtca cgatttcacc gcgcgccttc
     4021 gcggctgctg cgtcgagctc gagctcgagt cgcgcgtttc catctgcggc gatctgcacg
     4081 aacgccaggc cgccacccga gagcttgcac gtcgtgcgtt cgcccggctg cacgacatgc
     4141 acgaccggtg tgcagtccac cttctgatcg acatgcagct ccgcgagtgc cttctccacg
     4201 aactccgtca acgcgggctg ctcgaccacg acgcccttga ggcgccacga ccactggccc
     4261 ttctcggtcg tcacatcgat cggtagcttc gtgccatcgg ccagcgacgc ctcgcactgc
     4321 gcgttcatcc cgaccagctc ggcgcactcc gccttcacgg tgacgccgaa tcgtgccgac
     4381 aggtcgcgcg cgatggcggc ctccagcgga gacgtttttg cacctacatc ggagggccga
     4441 tcgcggcacc ccacgactgc aagcacgccg acgatccgga gtccgaaagt cacggaagat
     4501 ctcgtaacac gaggagaagt tccggagcct gcctacatgg tctggatgaa ttttccgagc
     4561 taaattgcgt cccacgttcg gtttcacgga ggaacgatgc gcaaccaact agctcgctgg
     4621 cttctgactg cgtctctgct cggtggctca ctgctgacga ccgcgtctgc acagcccaat
     4681 gttcgcgatc accgcaaggg tcacggcccg aagatcgacg ccaagatcga tgcgaaggtc
     4741 gtcgtcgacg tcaatccacg caccgctcct ccgccaccga aagccgagaa ggccggttcg
     4801 cgcggcggct ttgtctgggt cgcgggaaac tgggactggc aaaaaggcca gtgggtctgg
     4861 gtttccggcc actgggagaa gcatcgcaag ggcaagcgct ggcgtgatca tcgctgggag
     4921 aagcagggcg atgtctacgt gaggatcaac ggcgactggc aggacgatcg tccgaacgcc
     4981 gcgccgcctg ctctacaaga agagaagtgg gaaccgcgca cgggcttcgt gtgggttcgc
     5041 ggcaactggg actggcaaaa cggcaactgg acgtgggttg ctggtcactg ggagcgcgag
     5101 cgcgctggca agcgttggac cgaaggccag tgggtgctgc aaggcggcgc gtgggtttgg
     5161 acggatggct cgtgggccga cgctccgaag ttcccgaccg ctgcaccgcc cgcgatcaaa
     5221 gtcgagacgg tcgcggccaa gcccggctat ctctgggtga agggcaagtg ggactggcgt
     5281 aacggcgagt gggcctggac gccgggtcac tgggaaaaag agcgcgctca gtactcgtgg
     5341 caagacggtc gctgggagca gaagaacggc cagtggacgt gggtcgaagg atcgtgggtc
     5401 gcggcagctg cgccgcctcc ggtcgtgatc agcgttgtgc cgacggccgc gccacccgcg
     5461 ccgcaagccg agaccgtgag cccgcgctcg ggcttcatct gggtgcgtgg tcgctgggat
     5521 tggcagaagg gcaactgggt ctgggtcgct ggtcactggg agcgcgagcg cgcgcagtcg
     5581 aagtggatcg acggcagctg gcagaaccag ggtggcaagt gggtgtgggt cgaaggctcg
     5641 tggcagacga gcgcgccgcc tgcgcctgct ccgactccgg caccggcgcc gacgcccgca
     5701 ccgacgcctg cgcctcctcc ggctccgccc gtcgcgcaga ctcctccgcc gggcccgacg
     5761 acggcaccgc cgcaaccgca ggccgagaac gtcgcgccgc gcagtggctt cgtttgggtg
     5821 aaggggcact ggggttggtc ggcgaacaac aagtacgaat ggatcggtgg ccactgggaa
     5881 cgcgagaagg cgaacgtgaa gtggtacgac ggccattggg agtcgaagtc ccaaggcaac
     5941 gtgacgtact gggtttggat cgagggcggc tggaagtagt cagctagctc ttcgagtcgg
     6001 aagtgccgtg cttcaccgcg cggcattttt gttttcgggt cgcgctacct accagcgacg
     6061 ctcgaagatc ggcagtcgca cttcgacgcc acgctcgccg tagacgctcg acggcagcat
     6121 ctcgacgtcg ggcagctcga ggtgccactc gtgcgtgccg aggccgagct cgacgcgcgc
     6181 cgcgacacgt gccttgccgg cagcaaagtg ccaatcggtg tcgagcttga agcgcaggta
     6241 ctggccctcg ccggtgccca agcgaaaccg cgcgcggcgc ttgcggccgt cgaagcgaag
     6301 gcccatgacg tcgtcggaca agacgttgac gtgaccgccg atgaagttgc cgagctcggt
     6361 gagctgatcg gtgatctgct gcgagagctt gagctggtga ccggtcggca cgtcgtgcca
     6421 gcgcgacgtc agcgcgagct cgtcctcagc ggaggcgacc gctgttgtac cgacgagcag
     6481 taaggccacc gcggccgccc tcactctgat catgctaaag cagcggcatg aagctgcgca
     6541 atgccggcat cctcgtcgct ctgctcgtcg ctgtcggcgc gtgtaagaag tcggaaccgc
     6601 aggcgcagaa ggcgggctcc ggcgtcgcgc aggtcacagt cggatcggca gatggctcga
     6661 gcgctgcgac agacccgtgg gcgaagccga aggtcgcgaa ggatccgctc gccaagccgc
     6721 tgttctggtc gctcgagaaa gacggcaaga cggactacat cctcggcacg atgcacacgg
     6781 gcgtcgatcc agaagcgcgt ctgcccgaca tcgtctggaa gaagctcgac gaggcgaaga
     6841 ccttcgcgat ggagacgaac ctcgccgatg caggcaagct cgaagtactt cgcaaagacg
     6901 gcacgtcact cgaggacgag ctcggacccg agtattggaa gaagctcgaa gcggcgatcg
     6961 gagtgcagga ggcgtcgcgg ctccgcggct tcaagccaat gattcccgcg acgctgctcg
     7021 cgctgcgtgg tctgcccgag acgccgccga tggatggcgc gctccacggc cgcgcgttga
     7081 accagcacaa agagatcgtg ttcctcgaag acatcacggc gcagggctcg gtcctcgaga
     7141 aatggatgaa cacgaaagcg ctcaaagaca tcctcgatga tctcgagatc ggcgagaagc
     7201 gctcgaagga gatgttggca gcgtacatct cgggcgacga agcgcgcatc gaggccatct
     7261 cgaacgaaga gcgcgaagac ttcaagcgcc acggccgcag cgagaaggaa tacgacgagc
     7321 agatggaaga cctcctctac aaacgcaacg cgtcatggat cccgatactc gagaagatcc
     7381 acggcaacgc cttcatcgcc gtcggtgcaa tgcacctgat cggcaaacgc agcgtgctcg
     7441 acctgctcca gcagcagggc tacaaagtga cgcggattac gccctgaaca gcggccgtcg
     7501 ggctgaaaag aacgcttgtt caatagcgcg gcgcgcggct agagtccggg caacctcgag
     7561 gggggttgtt atgcaccgtc ttgcgctctc gacgttcgcg ctcgttttgt tctcgacgac
     7621 aaccgcgtgt ggcaaggacc cgtcgacggg tgacgacggc gtcgatgcag gcttcacagg
     7681 tgacagcttc tccctcaagt ggggccccgt tgacgtcgcg cccggcaaag aagacacgca
     7741 gtgcatctgg gtccgtctca acaacgagac ggaaatcaaa gtgcacggca tgcacaacgt
     7801 tctctcggcg tcgtcgcacc atctcatcgt ctacaaggac gacatggaca cgacggagca
     7861 gaccacgccc gtcccatgcg aaccgttcac gggcgcgctg aacacgacgg gcatggtcgc
     7921 tccgatcgcg atcacgcaaa aagccgacga caagatctac ttgcccgatg ccgtcgcgta
     7981 cacgttcgcg ccgcaccaga tggtcaagct cgagatgcac tacctcaacg cgagtgacgc
     8041 atccgagaac gccaccgccg acgtcgactt ctacgtcgcc gagccctcga cgatccaata
     8101 cgaagcctcg atcctcttca cgggctcgat ggacatctcg ctccctcccg gccaaacgac
     8161 aacgctgcac caattcttcg cggtcccgtc ctacctcgac ctctcgaagt ccaacatctt
     8221 cgcgattacg ggccacaccc acgcgctcgg caccgacgtc aaagtgggca tcggcccctc
     8281 gaagacgggc ccgttcagcg aggtctacgc gccaagccca ttctcgtgga gcgagcccga
     8341 aacgcgcacg ttcgacgatc cattcagcat ccccgtcggc ggtgggatgg acttctcctg
     8401 cacgtggaac aacacgaagc ccaccaccgt gacattcggc gaatcggcga caaaagagat
     8461 gtgcttcttc tgggcgtact actacccatc gcaaggcagc aaagtctgca tccacaccga
     8521 caagtacaac ggcctcaacg tgtgctgccc cggtgacagc ctctgcagct acgtcgaggg
     8581 aatgctctag ctgctccgtc cctcgcggtt gcggaggttg cggaagttgc gtccgtccct
     8641 tagcgcttgc tcgcggttgc ggaggttgcg gaagttgcgt ccgtccctta gcgcttctta
     8701 gcgcttgctc cgtccctcgc ggttgcggag gttgcggaag ttgcgtccgt cccttagcgc
     8761 ttcttagcgc ttgctccgtc cctcgcggtt gcggaggttg cggaagttgc gtccgtccct
     8821 gagcgcttgc tccgtcccgc ggaggttgcg gaagttgcgt ccgtccctta gagctgcgcg
     8881 agcagcttgc cagcttggag gaggaccggc ggcgggattt cgtgaccacc gttgaacggg
     8941 atgaactcga cctttgcgcc cgcggctgtg aggtcgtcac gaaggagctc tgcgatcatg
     9001 aaggggagaa gggagtcggc gcggccgtgc gattggagga tcggcgtgtt ggcgagcttg
     9061 gcgtagagcg ggcgccattc ggattctgcg atcagcgtgc cggacatgag gatgacggcg
     9121 gccggcgctg tggcgcgatg gagggcgacg tcgagggcga gcatcgcgcc ttgcgagaag
     9181 ccgccgagca cgagcttcga gtcgtcgacg gcgtagcggt ctttgagcgc ggagaggagc
     9241 gcgtcgattt gcgtgcgcac ttcggcgagg cccgttggga tttcgccgcg gcggtcgcgg
     9301 atcgcgccgg tgcggaggtc ttgctcgagg cgcgcgaggt cgaggtgcca ccacgcgcgc
     9361 gagtcgccgt agaggccacc tagctcgatc ggtgcggcgg ggaagacgaa gcgcacaccc
     9421 gcggcgccgc cagtcgccgc cggcagcggg atgtactgcg cgagcgcgac gaggtcgtct
     9481 cccggcgctc cgaagccgtg gaggaggacg cacgtgatcg gaccgttgcc gacgatgcgc
     9541 gtggtgagac ctgcgagctc gacagtttcg atttcggtac ggggcggcac ggcggcgtta
     9601 tacgtcgcga ccgtgcgtgg ggcgagccgt gaggaagcgt tactggctac ttcttcggcg
     9661 cgtcgacggg ggtggtggcg ccggcgggcg cggtctcgtc gctgaggttt gccggcatcg
     9721 gcggtagctc gttgatcgcg gggaggagtg cgatctcgat gcgccggttc ttttgcttgc
     9781 cgtcgttcgt cgcattggat gcgatcggat cgttggcgcc cacgcctgat ggcacgagcg
     9841 acttcgcgtc catgccggca gtgacgagca cgcgagtgac ggtgagcgcg cgcgccgtcg
     9901 agagcgtcca gttgtccgtc ggtatgccgc aggcggcgga cgcggcggag ccgcctgctg
     9961 cggcatcggc gcctttgggt ttgacgtact cgacgttgtc ggtgtgtcca acgacgagga
    10021 agcggcggtc gggcagcttc ttcaacaccg acgcgacttc gacgacggcg tactcgcctt
    10081 gcttggagag atcggcgctg cctgtctgga acagcacttc ggacggcagc gagatcacga
    10141 gctggccgcg gcgcgcgctc acgtgcagct ggccggtgtc gatcattttt gcgaactgct
    10201 tgcggatgtc ttcgatcgcc gccatgcgct tgtcggcttc agccttttgc gcgcgcagcg
    10261 cgacgagctc cgcttgggat gcggtgaggt tcttgtagcc ttccgtgttc gacttctcga
    10321 gctcggtgac tttcagcgtc gccgcgccga gcgcgtccgt gcaggtcttg acctctttgt
    10381 cgtgcttgga gcccgcgtcc tgggcagtct tcagcgtctt actgcacttg tcgtagtcgc
    10441 tgatcgcggt cttgccggtg ctgcggtact gccacgtgaa gtagccaccc gcgacggcgc
    10501 ccgcagtcat cacgagcgcg tagagccaga ggcgccaggg aaagccgcga ggcttcttac
    10561 cgctcgacga cggagtctct ggtggtcgtt gcgccattgg ggaacgatac cgccgaagcg
    10621 gtgtgggatg cgacccgcgg cgcagccagc gtctgctgca ttacgaagtg gaaatgggcc
    10681 agtcaagggt gcgcagatcg ggcgcaatcg ccgcaaagcc cgcgcgcagc cgcgagacgc
    10741 tgggacgctt gcgcggatcg cgaatgactg cagcgcgaag taagtcggcg agcggtgcga
    10801 cgcgtggatc gcgcgctgac atgcgagcca atacttcggc gcctggttgc gctgcgtagt
    10861 gctggtcgag gagctgctgc aagttctcgc cttggatcag cagcgtgccg gtgaagcact
    10921 cgaacgcgac gcacgcggcg gcgtacacgt cggtaccgaa tggctcgaac gttgtcccgt
    10981 cttcggacca gacttccgct gcgccgtagt gcgcgctgcc gcagccgacg cggatgtggc
    11041 gacccgccag gccgaagtcg acgagcaccg cgcgtccgga tccttcgcgc aacacgaggt
    11101 tcggcggctt cacgtcgagg tgcgcgatgc ggacggcatg catggcttcg agacccgcga
    11161 gcagatcgtc gacgatatcg actgcgcgcc gcatcgtgag attcgattcg agcgtgcgct
    11221 cgaggttcgt cccgcgcacg tactccatcg cgagaagcgg cttcggctgc gcactcgcgt
    11281 cgaacgtgat gaagcgcgcg agattcggat gcgcgggaag cgcgagcagc gcgcctgctt
    11341 cttcgcggaa cagccgctcg aactcctgct ctgacatcgc acgcgcggca tcgcccgagt
    11401 agtcaggcac cttgagcgcg acgagctcgc ggtccggacg gttgcgatgt tccgcacgca
    11461 cggcgaggag gacggagccg ccagcaccgc ggccaattgg ccgaacgacg tagaagccac
    11521 ctagcaggcg agatagcggc acccagcccg gcaacgacac gtcgctcgat gatgcgccac
    11581 gctcctcggt gcgatcgatc ggacgccgcg cgagccacac gagcacgcgc tcgatcgcag
    11641 cagcgagagc aggcggcagt gcactccgcg cgtgatcgat ggtctgcgcg atggcctcgt
    11701 cgagctggct gctcgaatcg cgcggcgcgt cgtcggaacg ctgcgatgca cgcgccgcga
    11761 gcacggtctc gatcgcacgc acggccgtct ccagctcgtt cgctagatcg ccggcatcga
    11821 ggcccaagcg acgacgcgcg ccatatgtac gacgcgctag cgcaccgagc tccgtcgcga
    11881 tcgagtcgag catgcccgtc tcgacggcct cctgactcgt cgatgacatc aggcgcgtga
    11941 gctggttgcc gagccgcgcg agcgcaagac gcaccgactc gacgcgcggc gactgctcgg
    12001 gaggcagcgc atcggcgagc tcgcccagcc gctcgacgac cgtgcgcacg gcctcggcat
    12061 cgtcggggtc agccgcctcc cacgtcgctt gattgagctg cgcgtatgca gcaaacgacg
    12121 cctcgatgtc ggggaccatc gatgcttcgc gcacgatcgc gaaatcgtcg tcgggaaatg
    12181 cgagcgtcca tgcgagcaag aggtcggtca cctctgcgtg gccatcgcgc aagagcgcgt
    12241 cacccgcgcg cgtcatcgcg gcccacacgg cgcgacgcag cgacgactga tcatctgccg
    12301 cgcgcttcat cagctggcgt acggcggcga ggcgcggctc gagatcggac tcaccgctca
    12361 cgcgaacacc gtcgaggaga cgcacgagtg cgcgcaagcg agcgattcgc aggccgccgt
    12421 gtgcgacggc gccggcttct gggcgcagct cgcgatcgag gagcgaacgt tcgatcgatg
    12481 ccagcgcttt cgcgaactgc tggcgcgccg gatccgtgtc gcctgaaagt gcgagcaccg
    12541 ccggcagcgc gttatcggcg agcagctcgc gatcgagctc gcgaaggagg cgcatcgagt
    12601 gccggcgatc gaccgggtcg tcatcgctcg cgcgctcgag ccaatccgcg ctggctccga
    12661 tctcgtcgac ggcgatgcgc gcttcacgga gtgccgtctt cacgtcaccg aggtcgagcg
    12721 cgttgcgtgc ggcattgatc ggaacggcga gaccttccgc cgcttgcgcg ccgccgagct
    12781 ccgcttcgag cgcgtagagg agcgcgaacc ggctgtcatc gatgctcgtc gcatcgccct
    12841 tcatctgatc gcgtgtccaa tcgagcgcca tcgcggtcgc atccggcagc agcggcgcgg
    12901 cctcctcacg acgcagctcc gcgagcgcct ctgcagcctc gagcggtgca cgtgcgacga
    12961 gcttcgtcgc gagcacgtcg gcgtcgtccg gtgccacgat cgccaaaccg gcgaggccca
    13021 agatcgtacc gcgcgcgata ccgggctcct tctgcgcgcg ctcgacgaga acatcgagcc
    13081 agcgccgcgc gccaccacct cgctcgagtg ctgcagccgc agaggcggcg gcacggcgct
    13141 gctcggtatt cgtcgcgctc ggacgtagct cgcgatcgat gtcgtcggcg agcgccgaat
    13201 cgacgtgcgc gagaatgccg cgcgcgatcg acgcgaaccg ccacaccagt gcctcgcgat
    13261 cgccgagcaa ccgtgcaaga gcctcgcgca gacctgcacg cgccaacaca cgcacgccac
    13321 cacgatcgcc gctgtcgtag cgacgcaccg cttcgcgcgc accgtgagcg agcaagcgtg
    13381 cagagaggcg gcgtcccggt agcgagcctg tcgcggggac ctcgacccag ctctgcgcga
    13441 gcgccggttg cgcgagcagg ccaatcgcga gctcgccgat catgcccggc gcagtgagcg
    13501 gagccgcgag caccacttcg agccgagcac gcaaaccttc gctcgcgacg atcttcggcg
    13561 acgtgcgtgc gagcgagatg agtaggcgca cgaacgtgtc gcggtcggct tcgcgcaaac
    13621 gcgtgagcac gcgccggccg agctcgcgtc gctccgcacc gttcggcaac gcctgtgcga
    13681 gctcgaacgt cgctattgcg gccgacgccg gtgacagcca atcgagatcg ccgagcatgc
    13741 cgtcggcaag tgcggtgcga accgcgagca ggagctgatc ggacgcgaga ccctcgagcg
    13801 gcgctggctc gcgctcggca atttcggcga gcatcgcgag gccctgccgt gtcacctgac
    13861 ggcgttcctc ggcgttggcc agcgtgtgca cgctagcgaa cgcggcgagg taggacctgc
    13921 cggtgcccat cccgacaatt ttaccgccgc tatgtcgccg gggtcagatc ttcgacggtc
    13981 aacgtctcct tatagaagac tgtaccgtcc gccccgaggc ccgagaacgt tagcgattca
    14041 ccatcgatgt cgacgagccc gacgttgaaa gcacggtggc gaaacagcac gccgtcggcg
    14101 gtgcgcatgg gcgtcccgat accgcgggag atgggcccca cctggaattc gcggataccg
    14161 cgctcgtggc ggtgggcggc gaaccagtgc tggtcggcgc tgacgaagag cacgccgggc
    14221 acgcctgcga acgcatcgaa catgcgcgcg cgctcggtcg tgaagctcga ccagtggtcg
    14281 ttgccgtcgc cgaaatcgag cggcacgctc gtgaacacga gcttgaacgg cgctgtcgac
    14341 gcgcgcacac cgtcgagcag ccacgcaagt tgcgtctcgc cgagcatcgt cttcgcgtcg
    14401 ttgtcgcgcg ctgcattcgc gctgcgaaac tggcgcgtat cgaggaggaa gcactcgacg
    14461 tgagcgcccc atcgccacga gcgatagcgg atgtccgtgc ggcgtagcgg gaagaactcg
    14521 tcccacgcct gcatcgcggc gacgtagcgg tccgcttccg tctcgcgata catcgcgtcc
    14581 caatcgttgc ggaactcgtg gtcgtcgtag atcgcgcgca agcccatctg cgtcagcagc
    14641 cgccgcacgc gctgagatgt ccgcgcctcg acgtgccgcg tccgataggt gtcgagcgtc
    14701 atcgctggcg ggccgttatc tgtgtacgga aaatcaccga tcgaaacgaa gacatcgggc
    14761 gacgccgcaa tcacgtgctc gacgagctcg ctgtcgaact cgggataggg atcaacgtcc
    14821 gccgagaccg cgatgcgaac cgggcgcgtg tcatcgggcg agggtgccgt gcgcacgtga
    14881 tgcacgagct ctgcgccggt atctgcaaga gctcgcacgc gatacagcga gccgggcgcg
    14941 agcccgccga catcgaacgt ggcgcgtcgc gcagcgtcga gggtcgcggc tgtctgcagc
    15001 acggtcgtgt cgccgacctg cacttcgagc gcaatgtcgc gggcgagctc agcccagact
    15061 gcgacgagaa acgaatccga ttctggctcg acgatcgcgg tggccagctg cgtacccggg
    15121 tcgcgcacca gatcgttgtc gccacacccg gtgacgagcg ctgcggtccc tagaccaccc
    15181 caccgaagca catcgcggcg agttggtgtc atccgattca tggtggacag ccgagcgtag
    15241 ccgtcgttca atccgaccgc catgaggagg gtgctaggct gcgtaggcct gtcgggctgt
    15301 cttgcgagtg ttctcgcggg ctgcgcattc gagcacggcg atctgggcgg atccgacgac
    15361 gacccaggta gcggctccgg ttcgggcgcc gtgcagccag gcgacttcga cggtgacggc
    15421 gtggcggacc tcgacgacaa ctgcaacacc gccgcgaacg cggaccaacg cgatcacgac
    15481 gacgacggcc gcggcgatcg ttgcgacgtc tgccctcacc tgccagacgc aggcggcgat
    15541 tcagacggcg atggcgtcgg tgacgcatgc gatccgaatc ccacgacggc gagcgatcgc
    15601 atcgcgttct tcgacggctt ctaccagatg ccgcaatggg acaacgtgca aggccccggc
    15661 atgtggcagg tgaacagcgg tgtgctccgg caaccgaagc tcgaggcgtc gcagctcgtg
    15721 cgcgatgacg accccgatct gggaaccgtg ttcgtcgacg cgcgcgtgca agtcaactcg
    15781 atggcgatgg gccccgctgc gcgccgctcg gtcggcctcg tcgtcggctt tggcgatgcg
    15841 aagcactact tcttctgcgg cgtcgcgcaa acgcagctcg gatccgaagt ccaagccggc
    15901 gccgagttca ccgacttctg gggaaccgcg cagtacgagt acgcaccggg cgccttcgcc
    15961 tcgcaggtcg gcggcggttg gctaacgctc caaacgtcga catcggtttc gtcatggggc
    16021 ggcacccata tcgactgcat gggccaccgc ggtcaggtga ccggcaccgc gcagttcgag
    16081 cacgacggta cgccgaccgg tgacatcggc ctgcgcacga acggcatcga tgcgagcttc
    16141 gactacgtgt ttgtcgtcga gaccgctcca agcggttcgt agcgccgacg gcggcacgcc
    16201 gcatgctcct cctcgatgca tgcagccgca caaagaatcc cgaggtcgca cgaatgcgaa
    16261 tgcgcgaaac cagcgcgagc gcgaagcgat gcgcgaggcc aaccgcatgc ctcagccgac
    16321 gatcgcggac atcgatccgg aagatccgac gcagcgcatc gatgacgcgg gccactacag
    16381 catcacggat ctcgacgtcg aagcgcagcc atccgctgaa gctgccgcgg acatcgacac
    16441 ggcggtcgcc gacgctcgtg gcgaagccga cggcgaagac aacgacgagg aggcgatgat
    16501 cggcgagccg gacgagacca cagcagatgc gtacgccgct ggcgtgaaga aagactccgg
    16561 cgagctctac ggcatccaca ctccccacgc cgccgacaac gatctcgaca agaccgacga
    16621 ccaagagtcg ttcaaagact ccgagcaagg cgagcactgg ctcgaaactc tcggcaagaa
    16681 agctgctgaa tacggcgccg ccgccgaaga agagctcgac gtcgtcgaca acagcgatca
    16741 cgacggcggc caccactcgt ccgaaggcgg cgatcgaccg gtcgccgaca aaggctcggg
    16801 cggccccggc ggcctgtaag tccgaagctc ggacttgtgg tcttaccgct aagggacgga
    16861 cgcaacttgc gcaacccgcg actgtcgctc cgtccctcgc ggttgcggat gccgctaagg
    16921 gacggacgca acttgcgcac ccgcgcctgt cgctccgtcc ctcgcggttg cggaagttgc
    16981 gtaagttgcg tccgtccctt aacgcttgct ccgtccctcg cggttgcgga ggttgcggaa
    17041 gttgcgtccg tcccctacgc ggtgctccgt ccctcgcggt tgcggaggtt gcggaagttg
    17101 cgtccgtccc ttattggggg gagcgaacgg cgacgtagag gtcgagcggc gcggagccgc
    17161 gatcactctc gaggtagagg cggcagccgt cgggcgagat ccaggtcggg tggtcggcgt
    17221 ttggcgagcc gaggacggtg agcgcgggct cgagggtcca tggggctgtc ttcgacgcgc
    17281 ggcgggtctc gtgaatctcg ttggcgtctt ggtagaacat cgttagctcg tcggggctga
    17341 tgacgacgcg gctgacgaac aggttggtgt cggccaggac ttccgtgggc ggcgagaagg
    17401 tggagccgtt gagctgcgcg cggaagatgc tgagcgtgtt gccgttgccg cgatcggagc
    17461 tgaagtagat cgcgctgccg tccggcacga cgtagacgtc ttcttcggag ctcgcggtgt
    17521 tgagctcggc gacgagtgcg acgccgctga agttggcgtc ggtgattgcg ccgcgcgtcg
    17581 ccttgtagat gtccttttgc gagttgccgt tcgtccgcgt gaagtagagg gtgaggccgt
    17641 tggctgtcac cgtcggagac cacacctcga cggcgttgtc gcccgtgagc aagaggcgcg
    17701 ctggcgcggc gaacgtcgcg gaggtgctcg cgcgcgtgga cttccagagg ccgaggaccg
    17761 tggccgcgcc gacgacatcg ctgctgaagt acgccgtgag ctcgtcgggc gagaggcgca
    17821 ggaatgcgtc gcggttgttc gtgttgagcg cggcgatcgc agttggagcg gtggagaatg
    17881 gcgcgcccca gcagacgggc gtgcgcgcgt cgggggcggc atcggccgag gagccgtcgg
    17941 cgcctgcatc gagatgacgg tgctcgtcga cctcgccaca ggcggccacg agaagcagaa
    18001 cgagcgcgcg tttcacgcgc cgagcttaac ctagatgtgc gagcgcggct gcggcgatgg
    18061 ctggcagcgc gggcacggca cggtgttgca catgaggcag aggatgtcct tgcatgtttg
    18121 gcactggaag cagcagtcgc ggcacagctg atggttctgc gtgcacgcga acatctgctc
    18181 gcgcttcgtc ggcgtcttgc agtttgcgca ttgcgactgg catgcggtgc aggttcgctc
    18241 gccgcacgtg aggcagagga ggcggccgca ggttccgcag tgcatctcgc agtcggagca
    18301 gatcttgtgc tgcgccttgt cgcattgctt gagcgcgacg ttttgtgcgg cgcagttcga
    18361 gcagacgttc gccgcggcca tggcgttcat cgacgtggcg gcttcgttga gatcgacgaa
    18421 gaactcttct ttgtcgatcg gcaccttgtg cttgttctcg tcgacccact gcgcggcgcg
    18481 gccgttgagc ggcgacttcg tgttgtagct ctggtacgcg aggatctcgc cgacgcgctg
    18541 gatgagcgtg tcgagcgctt cgctcgcggt ccaatcgtcg ccgatgcaga cggcgtgcgg
    18601 cgcgacgttc gggtggaaga ccggcgtcag catccggaac atcggtgcat cgcgcgggta
    18661 gccacgcggc atcgtcacct cgagccggtg ttcttgcacc atctcgatct cgtcgccctt
    18721 ctcccggatc gacttcacct tgagctttag gcggtagcga tccggaggat tgccttgcac
    18781 gccttcgatc tcgattttcg ggtgcaggtg agcgaggcgg cgcagcgctt cgtagtccga
    18841 cttgagccgg cggaggcgta cgttatccat gactagtcct tcgtcgcttt cggaagcagg
    18901 tcgttcaggc gatcgagctc gtggttctgc acggcgtcgg acagcgcgcg catcgcttcg
    18961 gatggcaggc tgatgccgtg cgcgaggccg ccttcgacct tcgtccacgt gttgagaccg
    19021 acgagctggc cgcgatcgtc gaataggccg ctgcccgagt cgccgtgggt gaccgggagg
    19081 tccgtgatga gcagctcgta cgtgcctgcc ggcgtgttgt gcggctcctt ctgcatcagg
    19141 tggcccttgt gcacgagcca gccgtcacgc agcgggtttg gcacgaacat gacggacgcc
    19201 gaggcctcga gcgcacccgc gttcggcgcg atctgcaccg gcggcggcgg attatcgatc
    19261 tcgaattcga tcaccgcgat gtcgagcggg ggcggcgcgg cccagcggac gtacgccttg
    19321 ccttgacgcg cgtcggagag ctgcacccag acgggctgcg cgtccttcgc gtgacgccac
    19381 gagccgaccg cggcgtatgg catcgcgacg tggctgcagg tgacgatcca cgcgcgcttc
    19441 gcatcaccac cgacgatcgc gcccgtgccc atcgcgagcg ctcgcgcatc gccggacgcg
    19501 tctggcgcga acacgacgac ggtcgctgcg acgatgcggt cgactgtgga cgtcgacgcg
    19561 aagccgagct gctctttggg cggcatcgac gagtacttgc cgccgaagct cgacggcatc
    19621 gcgtaccagt gaaacccgcc gatcacggcg gagcccaggc ccgcgacgat cgcgagcaag
    19681 tgagcgatga ggacgtgcgc cttgcggtac gagcgctgct tgcgcgtgcg aacgagcgcc
    19741 acgatcccga cgatgaatgt gccgataccg atccacagtg cgcgcgtgct cgagctgctc
    19801 agatcgtcga acagatcctt cagcggcccg atctcgcgcg gccgcagcag cagatagatc
    19861 atccaaccgg cgaacgctgc gacggcgagc gacagcagca ccggcgcgaa gccgagcgcc
    19921 ttctgccaca gcggctcgcc cgcggcctcg ccctctcgac gagtgactgc atcttggatc
    19981 gcctttgtat cgatcttgat gccgctctcg ccaatcgcgt tccaggcctg ctgcgcttca
    20041 cacgagtcgc agatctggcc gggcgcgagc ttcttcggac cagcgacgtt gcacttcggg
    20101 caaacgcggc tcgtcgcgat cggcgcctcc gacttgcgcc agaagcgctt cttcttctga
    20161 ggcgccgcgt cgctcaactc aaccacccgc cttcagcgcg acgatgattg cgagatacat
    20221 cgcaccgatc gcggcgctgc cgtagcccat cgcgagatac gtgccgcccg atgctgccag
    20281 gtccttgcgc ttgaaccacg cgtagacgcc tgcgagcggg ccgacgaccg gcgcgacaaa
    20341 gccgacgagc gagaagatga acatccagat gagcgccttg cgcgccgtat tgatgcggct
    20401 ctgcttgtcg ctccactcgc ggtactcctt gttcgtcatg ggatcggccc acgggaactt
    20461 ggcgccgcac gagcagacga gcaggctcga accgatctgc gcgttgcagt tcggacaggg
    20521 cttctgatcg ccccagccct gccccacgag caccggcacc ggcggcttct ctgcgaccgc
    20581 ggctttctta cagccgtacg tgccgcagcc gccgatctcg ttccagcagc tctggtggta
    20641 atcggtcttg cagtcggggc attgctgcga cgcctcggtt gctccaacga ccgtgtagca
    20701 aattgcacaa cgagccatga ctagctcctc accagcttga agacaccgat cgccagacag
    20761 acgagcgcga tcgattggaa cgtgaacaaa aaccgtttgc gctcgacgag aggtgttacg
    20821 cgctgcacgg tcacgtagcc gaccgagagc acgaacacga tcatcgcgag gaacaacagg
    20881 ggccccgcgg gatgaaactc gaacgccttg ccgacctcgc catgagcgag cgcgacgaag
    20941 ctgcgcgtca tcccgcagaa cggacagtcg acgccgaaca ggttgtgcac gaggcacatc
    21001 ccgccgagcg gacgaccatc agcgaggctc acgctgttgc cgtcaggccg aagccatgcg
    21061 gcaatgccga gctgagcagc cgcaaggcca cccagcgcga cgtccagcgt gcgacctcgc
    21121 aaggacatca ggagccgccc acgcggagca tgaggacgac ggcccagccg aggatgtcga
    21181 gcacgccgag caccatgccg gcggtcgcga gaccctcacc accgagcgtc gggtccatct
    21241 tcatcgcggc cttcgcggca ttcgccttcg agatcgcgag gggcccaaag atgatgccgc
    21301 agatgaacag gccgatgatg ccgaacacga gcgactgaga tgcgcctggc gcgttcgtct
    21361 tcggaccgtg gtagatgccg tcgggcgacg tgatgtgctt gcagttcatg cagatctgcg
    21421 agccgatcgg aatcgccgaa ccacaccgcg ggcagctcat gaggccctgc gcggcgagcg
    21481 ctgcctgctg cgagacctgc ggagcagcgc cgtaacccgg ctgcgcgccg taggccggct
    21541 gaccgccgta catcggctgc tgctgttgct gcatctgcgg cagcggtgcg ttcacgcatc
    21601 cctgcgtcgc gcagccacct ttgttgtccc agcattgacc gtgatgctcg ccctggcacg
    21661 aattgcacac ggcggcgtca tcaccgagcc tcagcgggat ttggcaccac ttgcaggggt
    21721 gcccttccat cttcgggttc gctttgaatt tacgaacggc ttccatttgc aggctcctct
    21781 tgtcttctct taaccgaccg gtgtgcgttt cgcgcgttcc tcgaaatcga gaatcgtgct
    21841 gccaatgatg atctgatcac ccgaaaccag tcgacgacga cggaccttgt tgccgccgac
    21901 tgaggttccc atacgactgc tcatatcctc gatctcgtag acggtgccca cgcgatgaac
    21961 ctgcgcgtgc gacgcatcga tctcggcatc cttgaacaga tagacgtcac tctgcggcga
    22021 gctgccgaga atcgtcggcg tcttgtagag aatgaacgac ttgccggcga gcgggcccgt
    22081 gcgaacacgc agccacgcgt cgcgaccgag gcgctcgacg agtgccacga agatgccgat
    22141 cgacagaccg acggcgacga gaccgacgag gcgcgacatg tcgccaccct cgaacatcga
    22201 cgccttccac cagcgatcga tcggatcgaa gaacagaccg ccgagcgcgc caccgagtgc
    22261 accaccgatg acggagttac gcagctgcgt cttcgtcgag cggatgatgt tcatgccaag
    22321 gcccgtcgcg cagccgatgc atgcccacgc agcgctgcgg cacgcggcga acacgaagaa
    22381 gtgccacgcg ctgacgtctt tgatcgtgac gatcagcttg tcgccatgcg cgagctcgtc
    22441 ttgcagcgtg ctctgcgaga tcgacaggat caaacctgcg ggcaggaacg cgagcgtcgc
    22501 gaacagcgcc gccatgaagc tgccgagcaa cgagcgctcg atcatgcgga tccagttgcg
    22561 cgtcgtgagg ccctcggcga ggagcagacc gagcgcgatg agcgccgcgg tcagcgggaa
    22621 cagcacgaac agcggccaca tcggcttgtc gacgacaccg cgcgccgtcg gatcatcggt
    22681 cggacgaatc gacgccgcga tgaggcgcga tccttgctcg aggccggtcg cgtcgacctt
    22741 cgtgccgact tggatctgat cgaacgacgt gaacgccggc tggccctgcg cgccgacttc
    22801 gaagagcgtc ttgtcgggat ccacgtagac ctcgttgttg ccgatcgtca gcgacttcat
    22861 gcccgggccc gtgtcgaacg gatccgagtt gatgagctgc acttcgccgc cgacgacggg
    22921 gaagtcgtcg atcttcggct cgagcatcat ccaacacgcg agcgcgccga gcaccgaggc
    22981 gaacggcaga tagaacaggc tgttcagcag cagccgcttg atcggcgaga ccggcggcgg
    23041 gtcggcgacg atgcgctcac ggccctgctt ctgctgccgc aggcccttgt cgacctcggg
    23101 gtcgaacagc tcgtgcgcct ggatgcgaat atgctcgtct tgctgctcgc tcattgcact
    23161 cctccagaac cggcggacgc ggtcgctcca ctacctgcac ctgcggccgg gggcgttgga
    23221 cccttcggcg cttcaggttg tggtgccaca tcattggcgc gtggcaacgt cgctgaactg
    23281 tcgatggact tcagctgcat ctgatactgg ctggtgttac ccgggtcgag acgggcagcg
    23341 gtctggagca gacgcgtcgc gcgcgccttg tccccgttct tggcgatatc ggccgcgagc
    23401 tctgaataga gcagaccaac cgcggcacgc tgctgcgcca cttcacgcgc gagatccgca
    23461 cggatgagac tcgtgctctt cgacgccatc tcgagaatct cgagcgtcgc ttgcgccgag
    23521 ctgcgatcgt tcgcgagcgt cgcgagctga ttgcgcagcg tgatgacggt ctccttgagc
    23581 ttcttcgctt gatcggtgcc cggttgcgcc ggcgcatcga gatccttcag cagtgcatcg
    23641 aggctcgtga ccgcatcctc gacgggcttg tgcagcgctt cgtcgcccat cacagagcgg
    23701 agcacgccga cgagctcacc ttgcagagat gcgagcgtgc tcgcctgccc ctccgccttc
    23761 gccttcacga tctcgatctg cgcttgctgc gcgatctcga cggtgttctt catgccccac
    23821 cagcggccga tgaaaccgcc gagcaacagc aggatcaaac cgatgatgac gatcgcggcg
    23881 aacgcgccgg gaccttcacc gagcagcggc tccgagctgc caccaccaga gctcttggac
    23941 tcgtcgtcct tgcgctcgac gtccttcttc tccggcgcgc cgctcgcgcg gttgccgtcc
    24001 cacgtctgct ctttggcgcc gatccaatag cggcggctcc gacgaatctc gttgtcgtgc
    24061 cacgtgaaca tgccgtgctc tttgctcttc gggtcgtaga cgaaggcaac ttggaacggc
    24121 aggttgaaga agctcttctg gatgaactga tcgcgatccg acaggaagac agagaagcca
    24181 ggatgcgtgt ggtaccagcc aaccaccttc ttatccttgt acttgccgtc catctccttg
    24241 ttgatgtggt cccacgtgtc gtgcgtgaac gtgagctccg cgcccttctc gtcggcgtgc
    24301 agcgcgtcga tcgtggtctc gatcttgaga tacggacctt ggtcgtcctt gtagacgttg
    24361 ccgacgagca cgccgccgat ctcgcgcgac gtgtccttcg tgccacgctc caccgcgcgg
    24421 tcgaacgctt cctcggagag gaacacgcgg tactccatct tgaccgcggg agccttctcc
    24481 ttcttcaggt cgctctcgtc gatcgcgcga atatccagtg agctcattgc agctttcctc
    24541 caccactcgg cgtccacgac tcggctagtg gaccgaggac acgttcagca tcgccgtcga
    24601 gcagccatgc ctgctgacgg tccatgcctt gacgagcgac gacaatatcg aacggcggca
    24661 acccgagctc ccccagtgtc agcgagaggt cgagactatc accgcgcgac accgacgagg
    24721 cgatctcgac gacgcggtga gctttgcatt tcgggcatgc ggcttgcgac tcacgcacgg
    24781 cacctagtac ggcgcgaccc ggctccgtgt cgccgcattc gggacacgtt aacgtcgtaa
    24841 tgatatctcg cgagaaatcg agggatgcgc cttcgccgag ctcacgctct gcgcgagcca
    24901 gaagatcgcc gagcttcgtc tcgctaccac tcccgatcgg gatgatctct ccgagattct
    24961 cgtgacccgc gcactcggtg cggcgcggat atttgatgcg gctcgcttcg gcgaacagtc
    25021 cgtcgagatg aagcgcttcg ccgtcgagcg tcggctggcc gtgcacgacc ttgatcgctt
    25081 cctgcacttc catcgatccg atgatcgaag ccgcgaccgc gctcgtcgga acgtgaccgc
    25141 gcgtgaccgc ttcgcgcgcg agcatcgcgc acgatcgccg ctctgccagc agcttgcgat
    25201 cggtcgagtt catcgtgcac tcgtagcagg gaccgttctt cggatcgaag acccgaacga
    25261 cgccactcaa ggcctcgatc gcgccatcga cccacttctt gccgacgcgc gccgcacacg
    25321 agttcatgaa cacgcgagct tcgcggttgt cgacggcgcc gatgatgaca tctgcccagt
    25381 ggaagacgcc gagccccgct ttgacgagga tgttctcggt atacgcgtgc gccttcacat
    25441 cggcgttgat ctcgcgcatg cgacgcacgg cgacttccgc cttgccctgg ccttcgtcgg
    25501 attcgcggaa caagaccgtg cgcgacaggt tgctgcgctc gatatgatcg ggatcgtaga
    25561 cgagcgtgta gccgctgccg atgagggcca gcagcttcag gatctcgttg ccgagcgcac
    25621 ctgcgccaat caccagaatg cgcgtcttgc gaacgcgctg ctgatcccac cactggatca
    25681 gcgactgccg gtgatagcga tcctctctta ggtcaacgct ctccaccggc cgtaatctca
    25741 ggttggatac gaacgatgtc gtcctcgacg cactgttgct gcgcgagcgt ctgatcgtcg
    25801 agcagctgca cgccgagccg gcgatggtgg aacttgtagc tcatcagctg accatccggc
    25861 gcgtagcgcg gcagatgcag cttgtcggca agcaccatga gaatgcgttc gaccgcaacg
    25921 tcgttcggaa cggtcacttt gagccgcttg ttacccgtcg cgtcccagac ttcgaggttg
    25981 atgtcaccca cagcttggct ccttactaca gatctactgg caatacgtgg cggtcagagc
    26041 gaacgtgtca aagtagaacg aagtggcgtt gatgacgtcg ttcgtcgtcg tgaatttgag
    26101 tcggacggtc tggctggaat agttgcctga gaatgttttc gagacggctg tccacgccgc
    26161 cgtcggggcc gcgttcgaaa ggctcgcgat cgtctcgagc ggcgtcccgt cggtcttcgt
    26221 gagcgacacc gtgccgcgat cgtagacggt ggttcctgtt tcgctcgtgc gcacgtcgta
    26281 gaagccggtg aataccagcg acgtggtgcc cgcgggaacc tggacatcct ggtagaggcc
    26341 atcggttgcg gacgacgcgc cgatgtcatc gcccgagatg ccgcccatcc atgccttgta
    26401 gggcgccgag tgcggggcga gcggagggtc atccgtgacg accggaaatg cgttgtcgat
    26461 gttctgcatc acccagctcg tccctgcggg cgcgagatcg agcgcgggat tgaggagtag
    26521 ttgcttcgtg tgaaccgtac agacgtacgc gtccacgggc ttcggtgcgt cgatcgaagc
    26581 atccgcgggt ggatgcacga tttgcgcatc gggctggggt tcgatgcgac catcgagata
    26641 aatcttggcg tcggccgggt gcccaacatc agcatccacc gcctgatcgt tggcccgacc
    26701 agatgcgcat cccacgagaa gcacagccaa gctcaccctc ttcatcggcg aggggcatat
    26761 cccgaggccg aacgggtttc aacgaacatt ttcggtggtg agccacgggc agtagacccc
    26821 taggccccct cccggtcgca cgaaaaatag gtccgactga atttcccgat ttctgggcct
    26881 aattcagtcg gaccgtggtt tcagaccgcc gtgcctgcca tcgcccgccg ggccgccgct
    26941 gccgcaggtt tccacccgcc ccggtagacc cggagccaga acagcgacgc gcccaggacg
    27001 atctcgatcg ccagcccgat ccagccaccg accaccccaa gcccgaggtg aaccccgagc
    27061 caccaggtga gcggcggtgt ggtgagccag ctcgtgatga tcccgacgat cgcggcgtac
    27121 cgaacgtcgc ttgcaccgcg cagcacgcca cgcgcgatga cgttcgccgc atcggcgacc
    27181 aagaacgcga gactgacgtg cacgagcatg gtggcccgcg ccgcgagcac gtcgtcacca
    27241 gcgctcatcg cctgcgcgat gtgagcaccg aggatggcat acgccacgca acagacggtc
    27301 gcgtaaccgg cgccgatcgc gagcgcgcgc tttgccacgc gtgccacgag cacgtcgcgg
    27361 ttcgcaccga ccgcctgacc gacgagcacc gctgcggctt ccgccatcgc atgcgcaggc
    27421 aagaacgaca cgttcacgag gtggagcacc atctgatgag ccgcaccatc gacggcgctc
    27481 atgcgagcga cgaggactgt gaggatcaag aaggcgccaa cctccatgat gaactgcaag
    27541 ccgttcggaa cgccctgcgc ccacacggcg cgtgcagccg acaccgacca cttcagcttt
    27601 gcgagctgca gacgcatcgg tatcgcgagc acggcgagct ccgtgacgtt gccggcgatc
    27661 gttgcaacgg ccgcgccttt cacgccccag tgcagaccga ggatgagaac ggcgtcgagc
    27721 gcgatgttga tcgcattgcc ggcgagcgac gcgcgcatcg gggtgcggct atcgccaacg
    27781 ccgtagctcg cctcgcgcag cgccgcatag acgaggacca gcgtcgcgcc aagtgatcgg
    27841 atcgcgaggt actgcgccgc gaagtgcccc gctctcggcg atgccgacag ctgcacgagc
    27901 agaggcgcga cgaagtgcgc cgtgatcgtt gcgaggatgc cgagcgcgac ggcgagcacg
    27961 accgctgccc cgagtagctc ggggatgcgc tcgcgacggc ctgcaccgac ggcttgcgag
    28021 acgagcgtct tggcaccgcg caacaggccg atgccgaagc acacgagtgt gaagccgacg
    28081 acgccggcga gaccaacgcc cgcgagctcg tccgctccga cgtgcgcgac gaacgcagtc
    28141 gaggccaagg tcatcgtcga gaacgagacc atcgagacag tgatgggcca ggcgagcttc
    28201 agtaatgcgc gcccgggatg atcggaccat gagagagagc tttgagagac agaagacgca
    28261 agcacgggac acccccaagg ggttgtgagc gcagctgcgg ccatgcggca gccaacgctc
    28321 gggtcgccag gtccttctcg ttcgccgaaa cgaagaaggg ctcctgggtg ttgaaccgag
    28381 gagcccgcgg cggttggttc ttactcgatc agcgaatcga gccctcacca ccgcgggcaa
    28441 tcccgcaatc gacgatgacc atcgtcggct gctggcgcgt aaagcggttg atgaggctca
    28501 tcgatgaatc tccgagggac actttcactc tcgctccgcg ccccgcgatc cgtcaagcat
    28561 cgtcttcgcg tcgcgacaaa acagtgcatt ccgtgacgca ggtcaatcct cggcgtcttc
    28621 agcggcctgg ctgaggtccc acgatcggcc ggtcttcgct aaatcccaga aaaagaacgc
    28681 gaggctatgc gcgacgcggc ggacgggtcc tccatcttcc tcgaacgaca cccagatctc
    28741 ggcgtcaggg gcgccatcgg caaacaccgc ccaccacgcg tgatcgccct gatgcgggag
    28801 catgatgcgc gcggcgtggc gcccctcgtg atcgcgcagt tgccaatcat cggcgccctg
    28861 cggctcggga ttgttgcagt gcgagtcctc gatcttctgg agcacgtcgg ctttgcccca
    28921 gagcgtctcg agcgtcgcgg gcagcttgat ccggtggcgc gtcgcgaatc gcgcgatcgc
    28981 agcatgctgc tccgggtctt cggccgcctc ggcaaggcca agcacgcgca ccgtgtcgac
    29041 gatcgtgatc gcgcgatcga cgtcgctcgg atcgcgcccg cccatgcgga cctgattcca
    29101 atagagcttg aacaggtcgc gcgacggatc gagcgacggg ttcgcgaata gcagcgcggg
    29161 cgcgccggat ccgacgaaga attcgtatgc acgttcgcga tcgagaacct cggccttcgc
    29221 ctcgcgatcc cagtacgcgt gccagctctc gtcctcgaac gtcgcgccga gcttaccgaa
    29281 cgccgcacgt gccgcagccg aatcgatgcc tgctgcgcca ccacgaaggc tgtcgaacac
    29341 gtcgggccac gccgctgcat cgtcgatgag gatctcgctg cgcgtgacgc cgtgccactt
    29401 cgccatgaag tcgtcctctt cgccgatcgg cacgatgtcg aacgtctgat cggggttgca
    29461 gcgaacgatc gtggagggcg tccaccgggt gccgagcttg acgatgcact tcgtgcccac
    29521 cgtggtcatt gcggcagcgt acagcggact gcgatttgcg gcgatacgcc gggaatgact
    29581 cggcagctct cccggttgac agggcgctcg cgccgcatca acttcgcccc ggtcattctt
    29641 atgtcctcga tctcagaaga cgtttcgaag ctccaaggtc gcttcaccgc actcaaggcc
    29701 gaggtcggca aagtcctggt cggccaggac gagatgctgc atcggttgtt gctcggctta
    29761 ctggcgggcg gccacgtgct cctcgaaggc gtcccgggcc ttgcgaagac gctcgttgtt
    29821 cgcacgctcg ctgacgcact cgacggcacg ttctcgcgca tccagttcac gcccgacatg
    29881 ctgcccggcg acgtcgtcgg cacgcagatc tacaacccgc gcgagggcac gtacacggtc
    29941 aagaaggggc cggtgttcgg caacttcatc ctcgccgacg agatcaatcg cgcgcccgcg
    30001 aaggtgcagt ccgcgctgct cgaagcgatg caggagaagc aggtcacgct cggcgatcag
    30061 acgttcaagc tgaccgagcc gttcctcgtg ctcgcgacgc agaacccgat cgagcaagaa
    30121 ggcacgtatc cgctgcccga agcgcagctc gatcgcttca tgctcaaggt gcgcgtgggc
    30181 tatccgacgc gcgacgacga gctgaagatc atcgaccgca tggcgggctc aggctccgag
    30241 ccgagcgcga gcaaagtgat gacgtgcgac gagatcctca gcgcgcgtga tgtcgtgcgg
    30301 cgcgtattcg tcgacgacaa ggtgaagcgc tatgcggtgg acgtcgtcgc ggcgacgcgc
    30361 gatcccaaag cgtcgggcat gccggcgctc gcgccgctca tcgataacgg cgcttcgccg
    30421 cgcgcatcga tcgcgctcgt caaagtcgcc aaggcgcagg cagtgctgtc gggccgcgat
    30481 tacatctcgc cgcacgacgt gaaaacgatc gcgcacgacg tgcttcgcca ccgcatcctc
    30541 gccagctacg aggccgaagc gcagggcaag accaccgatc acgtgatcgc gcagatcctc
    30601 gagaacgtcc cggtcccgta gggtttcgcc atgctccccg ccgagatcgc gcaggcagta
    30661 aagcgcatcc agttcgtcac gggtcggcag gtcgccgacg tgatggccgg cgcgtacctg
    30721 tccgtcttca agggacgcgg catggagttc gacgaggtgc gtccctacgt gcccggcgac
    30781 gacgtgcgat cgatcgattg gaacgtcacg gcgcgaaccg gcgagccgca catcaagcgg
    30841 tttcaagaag agcgcgagct gaccgtgatg ctgctcgtcg acgtttccgc gtcgcaggac
    30901 tttggctcgg gccgccgcag caagctcgaa gcagcggtgg agctctccgc gctactcgcg
    30961 ctctccgccg tggagaacgg cgacaaggtc gggcttctcg cgtttcactc gggcgcggac
    31021 ctctacatcc cgccgcgcaa gggcggcaag cacgcgctgc gcatcgtgcg cgaagtgctc
    31081 gcgcgcggcg aaggtgcagc agcaggcccg cagcgcttgc cgatgctgcc ggtattcgtg
    31141 cggcgctggt tcgcgaagct gcagaagacg acgcgcgcgc agccgagccc tcgccgctcg
    31201 acgagcattg cggctgcgct cgagttctgc cggcaagtct tgccgcgccg tgcggtgctg
    31261 ttcttgatct cggattttct cgacgaggga tatctgcagg tgctgcggca cgccaaccgc
    31321 aagcacgatg tcgtcgcggc gctgatcacc gatccgcgcg aagccgagat gccgcccgcc
    31381 gggctcgtca cgctcgaaga tgcagagacc ggggcaacgc ggctcgtcga cacgcggtcg
    31441 gcgcagtttc gcagcgagct cgcggccggc gcgaagaagc ggcagaaaga tctcgaggat
    31501 cagttgcgcg cgacgggcat ggatctcgtc gtgttcgacg cctcgcagtc gatggtcgat
    31561 ccgttgctgc ggttcttcaa gcagcgcgag cggaggctgc gacgatgagg tggctcgccg
    31621 tgctcttgct cgtcggcgcg tgctcgaacc ggagcgcgct cgattcgccg gatcaagcga
    31681 gcaagctcga gccgtcgaaa gacgcgatta cgaagacgag cgagaacggc ccggtgaagg
    31741 cgacggtcag cgtgtggcca gcgaagccga cgctcggcga gtcgatctat ctacggctcg
    31801 atatcgacgc gccggcgggg atcagcgtcg atgcgccgtt tcaagaagcc ggcgatcagc
    31861 ggctcggtcg attcaaagtc gtcggcttcg tgaaggacgc gaagcgcggc gcgaacggag
    31921 cgcagcacca agagcagacg tacacgctcg aagctccggc gagcggcaag caccgcatcc
    31981 cgccgctgcg tctcgagatg gtcgactcgc gcagcgacgc aaaggcggcc gcgcaggaga
    32041 tcctcaccga ggagatccca ctcgacgtcg cgccggtacc gacggaggcg atcggcaaag
    32101 agctgcacgc cgctgctggc aagctcgatc cggacgtcgg cggcacgccg tggctgtgga
    32161 ttggcctcgg cgcgagcatc ctcgccgttg cgttcgcggg ctcgctgctg acgtggcgtt
    32221 acttccgcgt gcggcgttcg ctcgaggaga agaagtccgc gtacgaagaa gcgatcacga
    32281 agctgcaatc gctcgaagcg cgtggtgcgc ctgcagccga gcaagcggac gcgtggttcg
    32341 tcgagctgtc cgcggtcgtg cgcgactatg tcgagggccg ctatggcctg cgcgcgcccg
    32401 agctgacgac cgaagagttc ctctacatcg ccgcacgctg gcccgcgatg agcgcgctcg
    32461 atcgcacaca actgtcgagc ttcctcgagc gctgcgaccg cgtgaagttc gcgggctacc
    32521 ggcccgaatc gcaggaatcg atggagctgc tcgcgctcgc gcgcaagttc gtcgaggaca
    32581 cgcggccgcg cgatgaagcg ccggcgacgg tcagcgcaga tggcctcgcg aagggagccg
    32641 cctagttcta tgtcgtggca gattcccggc acgaacgtcg agctcgcgca tcccgcgctg
    32701 ttcctcttcg cgttgctcgc gattcccgcg ttctggtggt cgcggcgcgg tgccggtcgc
    32761 gtcgtattct cgtcgctgcg cgcgctgccg tggggcggcc agacgtggcg cacggcgctc
    32821 gcgtgggtac ccgacgcgtt gttcgcgctc gcgttcgtcg cgcttgcgat cgcgctcacc
    32881 gggccgcggt ctggcgacaa gagctcgcgc attcgtcgcg aaggcatcgc gatcgtgatg
    32941 gccgtcgacg tgtcgggctc gatgcgcgcg ctcgaccttt cggagaagaa ccaagagctg
    33001 acgcggctcg acgccgtgaa gcgcgtgttc gaacagttcg tgctcggcgg caagcggctc
    33061 ggcggtcgcc ccgacgacgc gatcggcctc gttgcattcg cgcgctacgc cgatacgcgg
    33121 agcccgctca cgctcgatca cgcgaacctc gtcaatgcgg cacgccagct cgacttcgcg
    33181 acgcaagatg aagacggcac cgcgatcggc gctggactcg agctcgccgt gcagcgcctc
    33241 gccgagtaca aggcgcgcag ccggatcgtc gtgctgctca ccgacggcga atcgaacgtt
    33301 cacgacatcg acgaggacac cgcgatcgaa gacgcgatca aggccggcgt gaaggtctac
    33361 acgatcggtg caggaacgaa cggcgtggcc ccgatcgcgg tcgaccgcgg tgatggccgc
    33421 aagcagctca tgcagatgac ggtctcgatc gacgagaaga cgctcgggaa gatcgccgag
    33481 aagacaggcg gccaatactt ccgcgcgacc gactcggcga gcctgcgtcg catctaccag
    33541 cagatcgaca agctcgagcg cacgacgatc gaggagaacc gcttcctcga atacaaccag
    33601 tactacggca tcttcgtcgc ggcggcgggc ctcatgatcg cggccgcgct cgcgctgcgc
    33661 tcgacgttcc tcaggaggct gccgtgaacc tcacgtggca gcacctcgag cgcgtccacc
    33721 tcgtgtgggt cgtcttgctg ttcgtcgcgt gtctcgtcgt gctcgagatc aagtcgcgct
    33781 cgacgctgtc gtcgtttctg tcgccggtga tgcagcgcag gctgacggcg cagccgtcct
    33841 ttacccgcgc gatggtgaag ctcgcgctca tcacactcgc gatgctcgcg tgcgttgcag
    33901 cgctgatgcg gccgcaagcg cgtggcgaga cggagacggt cacggtctcg caggcgtccg
    33961 cggatgtgct gttcgcgctc gacgtgtcgc ggtcgatgct cgccgaggac gcggcgccga
    34021 atcggctcgc acgtgcgaaa gcggagatcg gccagctcgt cgccaagctc gagggacatc
    34081 gcgtcgggct cctcgcgttt gcgggtcgcg ccgtgcccgt ctgcccgctc acgccggatc
    34141 actcgtactt caacaccgtg ctcggcaccg tcgacacgcg cagcgcgggc aaaggcggct
    34201 cgcgtctcgc cgaagcgatc aaagccgcgg ttcgcgcgtt ccctcagggg caaggcgcaa
    34261 agctcatggt gctcatcacc gacggtgacg accaagacaa gccggagtac acgcaagaag
    34321 cggcgaaggc cgcgcgcgat gcgggcatca agatcgtcgt cgttggaatc ggcagtgaag
    34381 aaggcagcca gatcacgctg accgatccag cgacgggcgc caagacgaca ctcatgcacg
    34441 acggcaagcc ggtcatcagc aagctcgatg gtggtgcgct gcgcaagatc gcgctgacca
    34501 ccgagggtgc gtacatcccg gcggggacga gcgcgatcga tctcgactcg atcatggaga
    34561 gtcacgtgac gccgatcgtg cggcaggcag cggcgggctc ggtgcgtgtc gtgccggcag
    34621 agcgctacgt gtggttcgtg ctcgccgcgg tcgtgctgct cgtcgctgcg ctcgcggtcg
    34681 gcggctccgg cctcgaacgg aggacgcgat gagcacgatc gttcggacgc tggtcgttgc
    34741 gatcctcgtg atgtgcgcgt gcgcgaagag cgagcatcgc gaagcgcgcg agaagtacaa
    34801 ccaaggcgtc gagctcctcg tcgcgggtga tcacgaaggc gccgagaagg cgttgctcga
    34861 tgcgcgctcg acggcgggcg tggatcccga gctgcggttc cgcgccgcgt acgacctcgg
    34921 catggcgtat gccgcgcacg cggagaagct gaagaccggc aaggagccgg atctgaacaa
    34981 ggctctcgag ctcaccgagc aagcggtgtc gtggttctcc gacgccgcgc ggctgcgcaa
    35041 ggatgatgcg gatacgaaag cgaacctcgc gatcgttcgt gcgcgcgcgc agtcgctcgc
    35101 cgatgagctt cgcaagagcg aaggcaagct cgaagcgcgc ctcgacgcgg tgatcgccga
    35161 tcaacgctcg gtgctcgacg aggcgcgcgg cgcgtggatc gcgatcaaag aagccggcgg
    35221 caccgatcca ctcgcgcagc aaggcacgct cacgcacctc gccgacaaag agcgcggcat
    35281 cgtggcggag gcgggcgtga tcggcgacct cgcagactcc gagatcgacg agatcgcgaa
    35341 gaagaccgaa gacaagcgca ccgacgagga gaaagtgcgg ctcgtcgtgc tgaagaacct
    35401 cgacgtctat ctcgcggaag cgcgcggcaa gatcgccgag gcgcgcggca agctgcagaa
    35461 cctcgcggcc gaagatggtg tcgaccgcgc cgaagccgcg ctcatcgcgc tgaagcgtgc
    35521 gcgcgagcag ctgctcgatc cgatcacggt gctgcgcgag gtcgcgcaag acgagctgca
    35581 gctgctgcaa gagaccagcg cggtggccga cgtcgaggca ggcaagctgc agcagctaaa
    35641 gcccggcgag acgcccgtca ttccggcgtg gatgagcggc cccgcgctcg cgctgcgcca
    35701 gctcggcatg catgaccgcg tcgaagaagt gcgcgcgcgc ctcgctgccg cgacggaagg
    35761 gccaccacct gcaccgccgc aaggtgcaga tccggcgcag gtcgagcagc agcagaagct
    35821 cctcgagcgc gtcaaggtcg cgctgccgct cgtcgtcgac gcttcgacgg cgatggacaa
    35881 ggcgcacggc aagctgatcg acaagaagat gcaggaggcc gtgctcgccg agcgtgatgc
    35941 gcttgtggcc ctcgccaaag cgatcgagca gtttgccgat ctcaagcaga cgatcgacat
    36001 cgcgtcggag acgcagaagc agatcgtcgg cgccctgacc gagaagaaga acgcgaccga
    36061 gacgaaggaa ggcatcgccg ccaacctcgc gcgcatgcct cgcatcaagg agctgctcgc
    36121 cgacgaggtg gcgaagctcg agaagcaggc gcagcagccc gccgatccca aagccgatcc
    36181 gaaacagctc gaggcgcaga aacagcagat cgatcagatg aagcagcaga tggccgatgc
    36241 ggagaagctg cgcggcgagg ccgaaaagct cgtcgcagac accgacgcgg ccgtgaaggc
    36301 gaacaaggat ccgctgacac ccgcaaaggc cgcggacgcc aagctcgacg agctacgccg
    36361 catcttcttc tcggtgatcg agcacctcca ggatctcatt cgccagcaag gcgagtcacg
    36421 cgatcaaacg tcgacggcgc atacgcagac cgacgaggaa cgcgcgccga agctcccagg
    36481 tctcgcgtcg cgccaagacc aacacggcca gctcgccaaa gcgatcaccg acgcgctcgc
    36541 cgcacaagcc gatgccgccg cgaagggaca agacccgaat gcgcagcagc aagctaagag
    36601 catgagcgcc gcggccgacg aagtgcgcct cgcgcagaac gacatggcgg atgcgaagcg
    36661 caccctcgat aaggcggcct cgccgcagac gcagcagtcg gtgagcctcg agcccgtgct
    36721 gaaggcacaa gcctccgcgc tcgagcatct gatgaacgcc ctgaagctcc tgcagccgcc
    36781 gcagcaacaa aagcaagacg acaagcagca gcagcagcaa caacagcagc agcaacaaca
    36841 acagcagcag aagcaacagc aacaaccgca gcaaggcggc gccggtcagc gcgcacgcga
    36901 cgaagatgct cgtcggcaga aggaacgccg cgagcgagag cagtcgcaag gcgatgcggt
    36961 agaacaggac tggtgatgcg ccgcgctcgc ccgcactcgc gacgtcagga ctatggtggt
    37021 cagaccagca tagcttccgc cgtcgcgcgg cttgtcgcga actgcacatc gcggcgcgcg
    37081 gcgcgtgggc cacgcgttgg tgggttgctc gcgcggcttg tcgcgaactg cacatcgcgg
    37141 cttgcgacgc gtggaccacg cgttggtgga ttgctcgtgc tgttgctcgt gctcggcgcg
    37201 gcgcgcggag ccttcgcaca gacggcgcgg atttcgctcg acgagacgcc gcacgtcggc
    37261 atgccgttca cgctcgggct cgtcgccgag ggcttcgacg aggcgcccgc gccggcgcag
    37321 ccgaagctcg agattcccgg ggccgtcgtc actccgatgg gcgcgcagcc gaacgtctcg
    37381 cagtcgatcc agatcatcaa cggcaagcgg agcgactcga agcgcgtgac gtgggtgttc
    37441 cgctggcgcg tcgagatcaa caaggccggt cgcgcgtcgg tgccggcgct gacggtcgtg
    37501 cagggcagca agaaggcgac ggcccaaggc ggccaattcg aggtcgagtc tgttccgacg
    37561 accgacgaca tgaagctcga ggtcgcgctg ccgaatcgca cgatcttcgt cggtgaaacg
    37621 atcgaggcaa agctgacgtg gctcttccgc gtgcagccgc aacaagaccc gcagttctcc
    37681 gtcccactca tggcgatgga cgacttcatc gtcagtgcgc cacccgcgac ggatccgcgt
    37741 cgtgcgttga gcttcgcggc gggcacgaaa gagttgcagc tgccgtacga ggtcgacgag
    37801 acggacgtcg gcggtcagca gtaccaccgc gtcgtcgtgc gcttctacgt cgcaccgaag
    37861 aaggcgggta agaacgacat tcccggcgcc tcggtgaccg ctgccctgcc cgtcggtcgc
    37921 cctgacttct tcggcaatgc gccgtcgcgc atgttccgcg ccgccgatgt cgcgcgctcg
    37981 ctcgacgtca aaccgctgcc ggagaccgac aagccaccgg gcttcgccgg cgcggtcggc
    38041 tcgcaatact cgatcgccgt cggcgcgagc cgctcggtcg tgcagctcgg cgagccggtc
    38101 gagctcacgg tcacgatcaa gagcgaccaa cgcctcgaca cgctctcgct cgggcgcctc
    38161 gacggtccgg gtggcctgcc caaggacaag tttacggtcc ccgccgatgc accgacgggc
    38221 gagctctccg acgacggcaa atcgaagacg ttcaaggtca ccgcgcaggt gatcgcgccc
    38281 gcctccgaaa tcccggcgat cgcattctcg tacttcgatc ccgtgaagag ccgctaccag
    38341 acgatccaca gcgatccgat cgccgtcagc gtcaaaggtg gcagcgtcgt cggcgcaaac
    38401 gatgtcgtcg cgatgaacac gacgaagccc ggcgagaagc cgcgcgcgat cacgacgccg
    38461 gagaccgatg tcgcgctcgt cggcgcagac ctcgccctgt ccgcgcccgg cgccacgcac
    38521 gacaagccga tgggcggctc gctgctctgg ctgctggtcg cagcgctcta cctcgtcccg
    38581 ctcgctctcc tcggcttgcg cgtctaccag ctccgcaccc aaaccgagcg cgaagaagcc
    38641 tcggaagtcc gcgccgctcg caagcgcgtc gaacaagagc tcgcacgcgc cgccaaggag
    38701 ccagcacgcg acaccgctgg cgccctcgtc tctgcgctgc gcgcgttcgc ccgcgccgtc
    38761 gagcgcagtc ccgacgacga cggtggcctg ctcgccaaaa tcgagaccga atcgttcgcg
    38821 ccgtccgcct cgtcgtcgcc actatcgtcc gatctccgga cacgagtgga agacctcatc
    38881 cgccgctgga ccgccgaagc aaaccggaag cgaacatcga cgggcaccac gacagccgcc
    38941 gtcacgatca tcgtcgccgc cctcgcagcc ccgcacggcg cagctcgcgc cgagtccacc
    39001 gccccctcgc ctgccgctcc ctcgacgctg gcgacatctt cgacgctcgt cgctgatgcg
    39061 agcggcgccg cgactccggc gaacacggca acgatgagcg gcgatgcgct ggcgaatggc
    39121 cgcagcgcgt atcagcaagc gatgacgctt tccgacgcga gcgcccgcaa ggccgccttt
    39181 cagcgcgcac aggtcgcgct cggcgaggcg gcgcatgcgc atcccgactc gccggagctg
    39241 cttgccgatt ggggcaacgc cgcgctcggt gccggcgatc tcgggacggc gacgctcgcg
    39301 tatcgacgcg cccttgcgat cgacgccgag aatgcccgcg cgcgccgcaa tctcgagtgg
    39361 ctccgcagca aggagtccga cacgttccgg ccgcatggca gcgttgccgc gaccgatgcg
    39421 ttgttcttct ttcatacgtg ggcgcgaggc cggcgcgtgc tcgtcggtgc gttcgcgttt
    39481 gcgatcggcg tgctgttgct cgtaccgtgg ggcgcgaagc ggcggcgctg gatgcatgcg
    39541 ctcgcgatcg tcccggggat tgtctggctc gcgttgacga tttcggtgct cgtcgaggat
    39601 cggcatgacg aagatgcggt cgtgatggag ggcgtcatgc tgcgcgccgc tgatagcccc
    39661 ggcgcaccga cgacgctcgc gcaggcactt ccgcgtggcg ccgaggtcgc ggtggtcgag
    39721 cagcgcgacg gctggacgaa gatccggctc gcgagcggca ctgcgggctg ggttccgtcg
    39781 agcgcggtcg agagaatcca ttagttccga tcgtcgacga atccgctaga ctgcgcgccg
    39841 tgactcgtcg gctctatcac gaggatgcgt atacgcggcg gttcgacgcg caggtgatcg
    39901 cgcacgcgac gtacaagggc gagcccgcgc tcgtcctcga tcagacggcg ttctatccag
    39961 aggccggcgg ccaactcggc gatcgcggcg tgctcggcgg gcttgcagtt cgcgacacgc
    40021 aggagctcga cgacgggacg atcgtccatc tcgtgggcgg cgagctcccc gcgatcggca
    40081 gccgcgtcga aggcgagctg gattgggcgc gtcgtcgcca gcacatggcg cagcacacgg
    40141 cgcagcactt gctgtcgggc tctctgctcg atcgcgcgca ggcacccacg gcatcggcga
    40201 ggctcggcga gagcgcgttg acgatcgatg tcacgcgcga tcgcgtaccc gagccggagc
    40261 tcgcggcggc cgaggatctc gcgaacgatc tgatcgacga tgacctcgcg atccgcgcgt
    40321 ggtttccaac gcccgacgag ctcgcttcga tgaagctgcg ccgcgatccg aaagtgagcg
    40381 cggacattcg cgtcatcgct atcggcgagt tcgatttctc gccatgcggc gggacgcatt
    40441 gctcgcgcac gtcgcagctc ggtgcgctgc gcatcatcgg tgtcgagcgg tacaagggca
    40501 tgacgcgcgt gacgttcacg gccggtcggc gcgcacgtgc cgagctgttc aaccgcgatc
    40561 aggtattgcg cgggctcgcg acgcagttct cgtgcggtcc cgcggaggtt ccggcggcga
    40621 tcgacaagct caagcgcgat gtcgagagct cgtctgccga gctcactcag ctgcggagcc
    40681 ggctcgcgag cgcgatcatc gatgcgattc ctggcaccgg cccagcggtc gcgtcgatcc
    40741 cgggtgatgc ggagctcctg cggagcgtcg cggccaagct cgtgtccgcg ggtcgcgacg
    40801 ccatcctgtg tgcgcccgaa ccgggtggcg cgaccgtcgt tttgttccgg gctccggggt
    40861 caacgctcga ctgcggcgcg ttatggaaga aggtcgtggc gaaggcgccc ggccgtggcg
    40921 gtggtcgggc cgaaagggcc gagggccggc tgacggtcga gatccccaac tgggcagaat
    40981 tcatcgacga cttagcgtag ctgtgcggga ttgagacact gtgcaatcgg gcgcgcgcgg
    41041 cgtgctccga tgagacgcca agtctcagca agcacagacg tttgcgtagg cgcgcagttt
    41101 gaaaaggctg tcgtgccttc gatttctcga aggtcacgca tatgagtcgg attgtcggca
    41161 ttggcgcttc ggcaggtggc ctcgagtcat tggagaagct gttctccgcc atgcccgccg
    41221 acacgggcat gtccttcgtc gtcatccagc atttgtctcc ggatttccgg agcctgatgg
    41281 acgagctcat cgcgcggcat agcgacatgc ccgtcgtcgt cgccgaagac ggcctgccgg
    41341 tccagccaaa ccacatctat ctcctgcccc cgcgcaagga gatgatcatc cgcaacaaga
    41401 cgctgatcct cacggacaag ccgaacggcc tatcgcttcc catcgatttg ttcttccgct
    41461 cgctcgctca ggacgtcggt gcggagtcgg tcgccgtcgt gctctcgggc agcggcagcg
    41521 acgggtcgcg cggtgtgatc gacgtcaagc gcgccggcgg tctcgtcatg atcgagaccc
    41581 cggcgaccgc gaagttcgac ggcatgccga tgtcctcaca cgcgaccggc gtcgtcgatc
    41641 actcggccac gcctcgcgat ctcgcgcgct tgctctgcgg attgccgccc ctcgatcccg
    41701 tcgatgaaat ggaaggggat caggtcctca acgaggatcc ggcgatggac tcgctgttgc
    41761 gccagcttcg cgacggcttt ggcatcgact tctcgctcta caagatcacg acggtcgccc
    41821 ggcgcgtgca gcgtcgcgct gagctgatgc gcgcgcagtc gctcgaccaa tacgccgagc
    41881 atgttcgcaa cgatccgagc gagctgaaca cgctctatca agatctactg atcggcgtca
    41941 cgcagttctt ccgcgacccc gagtcgttcg atctgctcga gcgcgaggtc atcccgcagc
    42001 tcctcgacaa gattccaccc gagcaggaag tccgcgtgtg gagcgcgggt tgcgcgacgg
    42061 gcgaagagcc gtactcgctc gcgatgctgt tctacgagca gatgtccgcg cgccagcggc
    42121 cgctcaatct caagatcctc gcgacggatg tccaccagct ctcgctcgag accgcgagcg
    42181 ctggcatcta tggcgagcag caactcgcca acctcagccc gcaacgacgc gagcggttct
    42241 tcatcaagcg cgcgaccggc tttcaggtgt cgcccgagtt gcgatctctg atcgtcttcg
    42301 cgcgtcacaa cgtgacgaag gatgcgccgt tcacgaagat gaacttcatc tcgtgccgga
    42361 acatgctgat ctacttccag ccgcaggcgc agcgcacggt gatgtcgctg ttccacttcg
    42421 cgctggcacc gaacggcgtc ttgttcctcg gcgcgagcga gagccccggt ccgctgtccg
    42481 acgaattcat ctcggtcgac gagcactgga aaattttccg caagcgccgc gatgtcgcgc
    42541 tgctctcgaa cgtgcgcctg cccacgctcg gcagcccgat ccgccgccag ccgttcgagc
    42601 cgccccgccc gcatggtccc gatccgctga tcctcgcgac ctacgaccag ctgctcgatc
    42661 gcttcatgcc tccgagcctg ctcgtcgacg aggaccgcaa cctcatcgac agcttcggcg
    42721 gcgccgagaa gctgctacac gtccgcaagc gccgccctac gtccaacgtg ctcgacctga
    42781 tcgacggcga gctcaagaat gtgatcggcg gtgcgatgca gcgcgccgtc aaagagaacg
    42841 gacgcgtcgc gttctcgggc gttcgcatgc tcgagagtga cggcggcgag aagcgctgca
    42901 cagtcacggc cgagccgttc gtccatcctc gcaccggtgc gcgcagcatc ctggtcgcgt
    42961 tcctggacgt gacgccgatc gagccgttcg tcgaagccga agctccggtt gccgcgtcga
    43021 cgatgcagca cgaccaagcg tcacgcgatc gcatggagac gctcgagacg gagctcgcgt
    43081 acacgcgcga gacgctgcag gccacgatcg aagagctcga gacctcgaac gaggaaatgc
    43141 aggcgacgaa cgaagagctc gtcgcgtcca acgaagagct acagagcacg aacgaggagc
    43201 tgcactcggt caacgaagag ctctacacgg tcaacgccga gtaccagcag aagatcatcg
    43261 agctcaaaga gctcaacgcc gacatgcagc acctgctcga gggcacggat gtcggcacgg
    43321 tgttcctcga tcgcgagctg cgcattcggc gtttcacgga gcgcatcgcg tcggtgttcc
    43381 gcatccaacc cgacgatgtc ggtcgcaaga tcggcgattt ctcgcaccac ctcgttcgcc
    43441 cgacgctgat gcaggagatc gagaaggttc gcgcgcgcgg cgtgacgatc gaagaagagg
    43501 ttcgcgaccg cgacggaacg ccgtacttcc tgcgcatcct gccgtaccgc gtcacccgcg
    43561 ccggcgacgc gccgcgcaag ttggagtcag gggcggacgg cgtcgtgctg acgctgacgg
    43621 acatctcgac gctcgccgcg gcgcgttcgc gcctcgcgca gctgtctgca atggtcgagt
    43681 catccgagga cgccatcatt ggcaaggcgc tcgacggcac gatcatctat tggaaccgcg
    43741 gcgccgaaaa gctctacgga tacacggcgg ccgaagcggt cggcaagcac gcacgcatgc
    43801 tgctgccgcc ggagaagacc gacgagaccg atcgcttcat cagcacggtt cgcaagggcg
    43861 gcaaggtcga gcaggtccag acgatccacg ttcgcaaaga cggcactcgt ctcgaaatca
    43921 gcaagacggt ctcgcccgtg ttcaacagcg aaggggatct cgtcgcggtg tcctcgatcg
    43981 ctcgcgatat cagcgcgctc gtcgccgcac agcgtgccgc cgaggatggc caggaacgcg
    44041 ttcgcgcgct gctcgaatcg accgccgaag ccgcacgccg tcgcgagcag ttcctcgcga
    44101 tgctctcgca cgagctgcgc aacccgctcg ctgccgtgct cggcgcgacg tcagtgcttg
    44161 ccgcgaaccc cgagcgcgcg acgtccgaac ggtgctgcaa cgtgatcgag cgccaagcac
    44221 gacacatggc tcgcctgctc gacgatctgc tcgacgtctc gcggatcacc cgcggcaagt
    44281 tcgagctacg cagcgctcct ctcgatctac gtgccgccat cgatggcgcg atcgaatcaa
    44341 ccgctccgct gttcgagcag aagggcgtca cgctcgacct cgcgctgcct gatgagccca
    44401 tcccaatgac gggcgacatc gcgcgactcc agcaggtaat cgtcaatctc ctgtcgaacg
    44461 ctgcgaacta ctctgcccgc ggaaatcgcg tcgagctccg cgtcatgccg caaggcgacc
    44521 aggtgctgct gcgcgtcctc gaccgcggct tcggtatcga acgcgagatg ctctcgaaga
    44581 tcttcgagct gttcgtgcag gccgagcagc gcatcgaccg cccgcgcggt ggccttggcg
    44641 tcggcctctc gctcgcgaag agcatcgtcg agctgcacgg tggctcgatc gaagcgcgca
    44701 gcgatggtcc gatgatgggc agcgagttcc tcgtccggct gccactcacc aagccggcag
    44761 aaacgcgcgc aagcaagccg catatccagc gcggcccgcg tcgtcggatc gtcctcgtcg
    44821 aggatcagaa cgacgcgcgc gagatgctgc gcatgctgct cgagtcgctc gatcacgaag
    44881 tactcgacgc gagcgacggc gcctccggcg tagagctcat cgagaaggtg aagccagact
    44941 tcgcgctgat cgacattggt ctacccacga tgaccggcta cgaagtcgcg cagcgcattc
    45001 gccagagcgc cgacctccag agcatcatgc tcgtcgcgct gaccggctac ggcaccgcga
    45061 acgatgtcgt cgcagcgcat gatgccggct tcgatgcgca cctcgtgaag cccgccgatc
    45121 tcgtgcgcct gcagcagctc ctcgcgagcc acaagaagga cgactcgttc gattagtcgt
    45181 cagctctttt gcttgagcgg aacagtcact ttgagatcgc taccggcagt cgggtagcgc
    45241 gccttcatca gcacgttgcg caagcatgcg ccgagcggcg tcgcgttgac ggcctctggc
    45301 tcgatgacga ccgtctgcgc gtggcctgct gccgcgacgg tgatcttcgc gctcatcgcg
    45361 tccttgggta gcgactcggg gtgcgccttc acgcaggcgt tgaggtccgc cagcttctgc
    45421 tgcacggcct cgtcgtacgt tttgggacgc ttgtctcgga cgaggtttgg accggggcct
    45481 ggacctggct tcgtgtccgg cgtggccgac ccggtctcgt gcggtgtctc ggccgcagag
    45541 cccttggtcg gattggtctc atccatgttg taggtcgtcg tgtcgcccga gcccgtcgtc
    45601 tggatgggct tgtcgccgcc accgccgccc tcttgcgtga tcttgatctc cgccgggggc
    45661 tgctggttct gcatctgctt ccacacgagg aacagcgcgc cacccgcgat gaggaccatc
    45721 aggatcgcaa cgaccggcca gacgaagctg cgctgcgaaa ccatgacgat cggcggcgta
    45781 ttcgccgtcg gtggcatgac cggagcgccg cggagcggct ggagcatcgg cgcgctgccc
    45841 gtcggctcga tgccaaaggg cggcggcgtg cccgagctcg aatgccccgc aaactcctgg
    45901 ctgttttgcg gccccatcgg attcggcgca ggcgattgcg gacgcggcat ccatggaggc
    45961 gcgccttcga cttcggtctc gacggacgga aacatctcgt cgtcgccgtc gtcgacctgc
    46021 gtctcctcgt cgggcatcga gaacagcggc atcgtcgagc gcgccggaac gccgcggttg
    46081 atcacggccg agacttgctg gtgacgcttg ccgatctcgt cggcgaagta atgcgccacg
    46141 aagtcgctca tctcgccctg cgtccacggc cgcgagtact gcgccatgcc gtcgagaacg
    46201 gccgagccga actgacgtgc ggtctcgaat cgatcattgg ggtcgcgctc gagcgcacgc
    46261 gtcagtgctg cggcgacgcc gtcgggcaaa tccggtcggt aacgacggat gtccggaatc
    46321 ggctgctcca tcaccgcgcg gaacgtcagg taatccgtct tgcgttgaaa gagccggcgc
    46381 agcgccagca tctcgtagag cacgacaccc atcgcgaaca cgtcgacgcg acgatcgatg
    46441 cccgagccct tgagctgctc gggcgccata tacgcgtact tgcctttgac ggtgccttct
    46501 tgcgtctgcg tcgacgcgcc gcgcgccttc gcgatcccga agtcgagaac cttagcgacg
    46561 cccgactcgg tgacgaagat gttcgacggc gtgacgtcgc ggtgaatgat gccgagcgac
    46621 tgaccgtcgc gatctttcag ctcgtgcgcg tagttcatcg cgtccgtcgt ctgctgcatg
    46681 acgccggcga taaacccgag ctcgagctgt tgctggttct tcgagaaccg ccggagtagc
    46741 ggcaagagcg agacgccctc cagatactcc atgacgatgt agagctgacc gtccgaatcg
    46801 ccgagctcga acacctggca gacgttcggg tggctcatct tcgaggcgat gcgtgcctcg
    46861 gcgatcagca tctggcgaaa ccgtgtgtcg tccgcgagat gcgcgaggat gcgtttgacg
    46921 acgtagagct tctcgaaacc ctcggcgcct tcgagtcgcg ccaaaaagat ctcgcccata
    46981 ccgccggtcg cgagacgagc taacagctca tatcgcccga gccgcgactg ccccccagtc
    47041 gctgcggcgc tcgcattcgg catccagctg taaagtctag cagagcactc cactcgaatg
    47101 cctcatcgac gcccgcccga gaccgcctac atctgcccac gttcagctgc tagggttgcg
    47161 cgtccacgtg cgcttcgagc ttcactgtca ttccaactgc tccgacggca ccgaaacgcc
    47221 ggacgcagtt gctgctcggg cgatcgcgcg caacgtcgaa atcttcgcac tcaccgatca
    47281 cgacacctgc gcgggtaccg tggcaaccag aacggggcaa agcgccacaa tccgcacgct
    47341 ccgcgccgtc gaaatctcct gcgacgatcc cttcacgggc cgaacgctcc acgtcctcgc
    47401 ctacgatcgc ggccacgccg gctgggctga cctcgagctc aagctcgccg acctgcgcga
    47461 agcgcgccgc aatcgcatgc gcgtaatggc cgccaagctc gcccaacgcg gcatccgcat
    47521 cgacgtcgag ccactcatcg cggacgccga ggcttccggc cgcacgctcg gtcggcctga
    47581 tctcgcgcgc ctcatgatcg cgcacggcgc cgtcagctcg atgaaagaag cgttctcgcg
    47641 ccacctctac gacggcggtc ccgtcgacgc tccgcatcgc gcactcacgc tcgcggacgc
    47701 tctcgcgctc ggccgctcgg caaacgccgc gatgtcgctc gcgcatccgc acctctacga
    47761 tcacctcggc gtgcggctgc tccgcgaatt cgcgaaagag gggctcgtcg gcgtcgaggc
    47821 gtactacgcg gcgtacgcgc cggccgaacg caatcgttgg ctcgatctcg cgaaggagct
    47881 cggcctcgtc gcgacggcgg gctcggactt ccacagcccc gacgatgccg tcgcaccggt
    47941 cggcgtcgat gtgcccgacg atcacggcaa gcgcctgcgc gactggctgc tcgacgccta
    48001 ggtcgtggcc gcttcgtcga gcagcagccg atgatattcg ctgagggcgc gctcgtagcc
    48061 gtcgaagatg gccggcttcg caacatcgag tgcacagccg agcatcgtcg cgagtgcggc
    48121 cttcggatgc cccgcgtcgg cgagcgcgag cgcgagaaac gcgacgagcg cgggatagtc
    48181 gggatacgcc gcgacggctt cgccgagaat cgcgaccgcc tcctcggcac gcccaacgtt
    48241 tcgcaacgtg gaggcatagc ccacgaggaa tcgcttgcgc tgcggctccg gcacgcccgc
    48301 gcgccacgcc tcgtcgtaga acttcacggc ctccttctcg cgcccttcgc gatcgtgctc
    48361 gtacgccttt gcgatcaatt cctcgacggt catcgcttct tcttctgctt cgccggcttt
    48421 ttggccggct tcttcacggg cttcttcttc gctggcttct tctttgcggg cgctttcggt
    48481 ttcggcgctg gttctggcgg cgcgggctcg ttggccatcg cgacgatgcg gcggtactgt
    48541 gcttcggtga ccggctggac ggagaggcgc atgcccttct tgagcagcgg catgtcggcg
    48601 agcgacggct cggcgcggat ctcccagagc gagacgaagc gcggcaggcg cctgacgaat
    48661 tggacctcgg cgcagtccca gatcggcttt tcgcgcgtcg ccctttcgtc gaagtacttc
    48721 gactgcggat cgaactgcgt ctcgtcgacg aggcggatgc gcgtgatccg cgcgaggccc
    48781 gcgacccctg gcggctcgca gctcgagtgg tagaacagga cctcgtcgtc gatgttcatg
    48841 ttgcgcatgt ggaagcgcgc gaacatcgag cgcacgcctg tccacggttc gatgcggacg
    48901 cgctcgagat cgtcgaacga gaacgtctcg ggctccgtct tcatcaacca gtactgcttc
    48961 gacatggcac tccgagatcg agaacgtgaa gcggaacgtc gtgggacgcg gcgtggactt
    49021 cgtcggcgaa gcccgtgagg tcgacgttga acgagaaccg agtggccgcg tcgagcgggc
    49081 ggaagaagtt gtcgtagtgc gtcggcacga tcatctgtgg tgcgagcgcg cgaacgatcc
    49141 gcgagacgta tttcggcgtg aagcggcggc ccgagatgcc gcacaggaag acgtcgacgt
    49201 tcttgtcgcg gatttcggct tcgagcaggt cggccgagcc ttggtgatag aagcgcatgc
    49261 ccgcgacctc gaggtggatg ccccagactt ggccgcattt gtaggcttgc ggcgtgagct
    49321 cgtcgaggtg ctcgcacgtg agctcgccgg agtacgggat gccaaggccg agctggagct
    49381 tgctgtgcac cgacggcacg aagtgaaagc ggaacgggcc gacttcgtag tcgcggtgcg
    49441 cctcgacgac cgttgcttgc ggcgcgagac catagaggcc catgaggttc tgcagcgact
    49501 gggagccgta caccgtgcac cccgtgtgct tggcgatcgc gggcacgtcg agcgcgtgat
    49561 cgaagtgcgt gtgaccgacg aggaccgcgt cggctttgtc gacccaccgc gcgacggcgt
    49621 cggacgatgg cgggaccacg cggcggagca ggatgtcgcg cagcggcaag cgcgtcacgt
    49681 atggatcgat ccagaggacg gtgccttggt aggcgaagcg gaagcctgcg gtgccgagcc
    49741 acgtcagctc gaggcccttc gggagcggca gcggagtcgc atgctgccga gcggttagcg
    49801 aatcgctctt ttgttggcgg ctcgccgtcg cgaagtacgc gagtccagca ccaacgtgtc
    49861 gaagcgggcc tagcaccgcc ccggattatt gactacaacc gggcctcaac gaaggagcga
    49921 ccgatggctt tcacgctgcc cccactgccc tacggtaagg acgcactgtc cccggtgatc
    49981 tcgccggaga cgatcgacta ccactacggc aagcatcatc aggcgtacgt caacaacctg
    50041 aacaacctcg tgcccggtac gaagtacgag aacatgtcgc tcgaagaggt cgtcaaggcg
    50101 accaacggcc aggcgtcgga gaagaaggtc ttcaacaacg ccgcgcaggt ctggaaccac
    50161 acgttctact ggcactgcct cgcgccgaag gccggcggtg acccgaccgg caacatccag
    50221 gcggcgatcg acaagtcgtt cggcaacttt gccgacttca agaagaagtt caacgacgcg
    50281 gctgccgcgc agttcggctc gggctgggca tggctcgtca agagcggcga cggctcgctc
    50341 gcgatcgaga cgacgtcgaa tgccgagacg ccgttcgcgg ccggcaagac gtgcgtgctc
    50401 acgtgcgacg tctgggagca cgcgtattac atcgactacc gcaacgcgcg cccgaagtac
    50461 ctcgaagagt tctggaagct cgtgaactgg gagttcgcga acaagaactt cggctaattc
    50521 gctgctgcgg atgcgagcgc gcgcacgcgg cgccagcctt cacggaggcg cggcgccctg
    50581 cgcgcgagtc gttcgcgcat ctcgatcgct cgtggatgat cgacaacgcg ctcgatgagt
    50641 gcgcgtgcga tcgtcgcgac gagcggctgc ggcttgtgcg cggctggcgg cggatccaat
    50701 gcttcacgga tcgagcgcag catggcgcct tggaacgtga cgggtttggc gcccggcgcg
    50761 ggcagcgcga gccgatccat gcgacccatc gcgtcatcgc gctcgtcggg cgtcgctcgc
    50821 tgcatggcgc gcgcgagcgc ctcggccgca gccacgatgt cctccgccgc gtccaccagc
    50881 tgcaccggca ctgggcagct cggacactgg gtcgaaatcg cgacgttata ggcgcgcttg
    50941 cacgtcgggc agtgcaggta gctcatgctg agtcccacgt tcggcggctt gcctacccga
    51001 ggcaactttt cgtcaccgga acacgcgtcg agaatcgcga gcttgcgggc gtgatcccca
    51061 tggcgagccg cttgctgttt cgcgactcgt gcggctcgtc gttcttacgc tcgcgctcgt
    51121 cgcctgtcac cccgggcaga cacctcgcga gccgctcccg ctcgtgtgca gcgaggaccg
    51181 cgtgggcaac gtcatcgtcg agggcggcac gcgggcggac gtcccggagc tcgccgtgct
    51241 cgaaggcacg ctggatgacg ccgagcgcac ggatcggatc gtccaggtct cgaaggacct
    51301 gctccatgca cgcggctact cgcgcgcgga gatcgaggtc gagcggcgcg cgggttgcgg
    51361 cgtcgagctg gtcgtccacg tcgatcgcgg tccgcggttt cgcgtcgcga agatcgcatt
    51421 cgacaccgat gacgagtttc cggcgaaggc tcggtatgcc gcgatcgaag atgcgctcgg
    51481 cacggtcaac gcggtcggcg gatcgctggt tgtcgatcgg cttggtcgcg cgctcgaagg
    51541 gctgaagcgg cagtatcaag atctcggttg gctcgacgtc gagatcgagc acgccgatgc
    51601 ggtgtacgac gaagcgagcg gcaaggtctc gctgacaatt ccgatccacg cgggccagcg
    51661 ctacaagctc ggcaacgtcg ttgcccgtgg cggcaggcgg tcgtcgcgag ctgccgtcat
    51721 cgatgccctc ggcctgcgcg gcggcgattg gtacaacggc cgcgccgtgc gcgaagcgat
    51781 ccgtcgcgcg agacgtgaga ccggcgaacg catcgaggtc aggctcgaag tctccgaggg
    51841 cgcagccgcg atcgatctcg aagcggtgct gcgaggcgtg aagtgaagcc atccctgttc
    51901 gactggctca cgttggcatt tgcggtctcg ctcgcagcgc tgtgcatcgt gctcgccggt
    51961 tgcagcgcga acgcgcacta cctcggtgcg ccccttcctg ccgcatgcag caagggcgat
    52021 gtcgaacgct gtgcggggtg gatggccgag cgcgatctcg tcgccggtca gctcgacgtc
    52081 tacgatgatc cggatctgcg cgcgtacgtg cagcgcatcg ccgatcgact cgcgcagggt
    52141 gcaggcctcg agcgtggacc acgcatcgtg atcgcgaatc acgacgggac gtatgccgcc
    52201 tatggcgagc gcatcgtcgt cggacgcatg gcgatcgaga agctcggcag cgaggcggag
    52261 cttgccgcga tcgtcgcgca cgagctcgcg cacgtcgaag gccaccacgc atcgatgaca
    52321 ctgtttggcc ccgacgccga cgcggtctgg ctcgccgccc gtcgcgacgc cgaagcgatt
    52381 gccgacgaac gtgcggtcgc gctgctcgaa cgcgccggct acacgccgtc cgcgatgcgt
    52441 cgcgcgctcg ctgcctcact cgacgccgac gacgacgagc acccgccgaa gacggatcgc
    52501 ctcaccgcgg tctcgacgct cgcggccggc cgcaccgatg gcttcgacgg ccgcgacgag
    52561 ctgctcgccc acgtcgacgg catggtcgtc ggtcgcgacc cgcagctcgg catgcgcatc
    52621 gacaacgcgt gggtgatcgc gttgctcggc gtcgcgatcc agctgccgga gcgcgatgtc
    52681 gttcgcgtcg acggcgacga gctcgtgctt cgccgtggtc gctccgcgct gaccgcgtat
    52741 ccgatcggcg cggcgtgggc gcgcgagctc gcggcatcgc tcgacgagcg cgcgagcgtc
    52801 gatacacgcc tcggcccgat caccgtcggc gttacgccct cgcccgccga aactgcgacg
    52861 ccgatcggca agctgcagag cgccgtccgc gagctgctgc ctcagccggc gccgggcacg
    52921 tgggtcgtca tcctcgtacg cccgggtggt ggcctcgtcc tcgagctcgc cgcgaagtcc
    52981 gatccattcc tgcgcgatcg ctggctgtca ggcctgcgcg ccgcatcgcc gtcggagctg
    53041 ctcgcgaccg agccgacgcg catcgcgatt catcgcgcgc ctcgcgcggg tcgtgtcgcg
    53101 gagctcgtcg aggaatgccc cgatccgtat cgcgcgctcg cgctggaaga tccggatcgc
    53161 atgctcgccg agggtcagcc gttcaagtgc accgaccgct gacggtcgcg cgaactcacg
    53221 cgcacggcat gacgcgcgga aacgcgacgc gctgaatcgt tccctgctcg gggctgacga
    53281 gcaggacgcc gtcgacggca aacgcgacgt gatactcgag gagctgccgg cgcgtggccg
    53341 cggtctcgat gctcggtgcg agctcaccgg tcttcacctc ggcgacgagc agctgcccat
    53401 ctccctcgac gagatagtcc gcgcgcagct cgagcagctg cggctcgcca tcgaggagcg
    53461 gagcccacag cacgcgcgcc tgcttctcga ccacgcgata ccccgcctct tcgagcatgt
    53521 ccgccgccgc atcttcgccg gcgcccgccc tctctgctcg gcggcgcgct cgccacgagc
    53581 cgcgccatcc gtgaatcgct tgcgcgatcc agagcgcgac gagcatcgag gtgagggcga
    53641 cgaggatttc gcggtacgga gcgatggtgt cgaggaattc gcgcgtcatg gaaaaattgt
    53701 cgatcgcgcg tctgacatcg cgttcgtccg gtttccggtc actccgccga acgcatatga
    53761 gagctgatat agaagtgggc gctaatgccg cggatcgagg tcattcaggg cgacatcacg
    53821 aagctcgatg tcgacgcgat cgtcaatgcg gcgaactcgt cactgctcgg cggagggggc
    53881 gtcgacggcg caattcatcg cgcggcggga ccggacctcc tcgagcaatg catccgcatc
    53941 cgcaacgcag aaggtggctg caagaccggc gacgcgaagg tcacgagcgg tggtcgcctc
    54001 aaagcaaaat gggtcatcca cacggtcggc cccgtctggg acggcggcac ggaagacgag
    54061 cccgagctgc ttgcgagctg ctatcgcaac tcactgcagc acgccgcgga gctcggcgcg
    54121 aagacgatcg cgttccccgc gatctcgacg ggcatctacg gctaccctct tcgcgaagcc
    54181 tgcgagatcg ccgtcaacac gatcagcgcg cacgccggcg atgcacccga gaaggtcatt
    54241 ctctgcatgt tctcgaagaa ggactacgac acgatgctcg aggtggtgga gggctagcga
    54301 tgccgaagat cgagtggatg caggcggaca tcacgaagct gcgcgtcgac gtcgtcgtca
    54361 acgccgcgaa cgagacgctg ctcggcggcg gtggcgtcga tggcgcgatt catcgcgcgg
    54421 cgggcccgca gctctatgac gagtgcttgc acctcgacgg ctgcgaaacc ggcgaagcga
    54481 aggtgaccaa aggctatcgt ttgccggcga agcacatcat ccacaccgtc ggccctgtgt
    54541 gggaagaagg cgcgcaaaac gagccggcgc tgctcgcgag ctgctatcgc aagtcgctgc
    54601 tcctcgcgca gtcgctcggt gcacgctcga tcgcattccc tgcgatctcg tgcggcgtct
    54661 acggctatcc aattcgcgac gcgcaagaga tcgccgtgaa gacgatcaac gcgcacgcgg
    54721 gcccgctacc cgagctcgtc atcctctgca cgttcgacga cgagacgacg gagatcacgc
    54781 gcgaggtcgt tgcctccgtg ctcgcgtact aggcaggctt cggatctggc ggcgcgatcg
    54841 gcaggcggtc gctcaccgac gcgtcgtcgg cctccgcctt cttgacgatc gtcgcgaggt
    54901 cgtcggcgat cacgccagcg tccttcgcgg gtagatcctc ggtcagctgc tcgtagactt
    54961 tcgatgcttt ctcgtcggtg tgaagggtcg cgtcactcgc gggcgtctcg ccaggcagat
    55021 cgacgcgtag ctcgcgggcc agcatctgca gtccagcggc gatcgggagt gcgagcagag
    55081 cgccgacgat gccgccgagc gtgccaccga cgagcaacgc gatcaggacg atcgcgggag
    55141 gcaaacgaag cacccggcca tacacgcttg gtaccaggat gcggctctcg aactcctgat
    55201 agacgatcat caggacgatc acgatgatgg tcgcggtggt gccgcgttgg gtcgccgcca
    55261 gtacaaccgg ggtactcgcg atatagccgc cgacgaacgg gatgatgtcg gtcaagccgg
    55321 cgaacaacgc gatcgcgagt gcgttttcca ccctgaggat cgtcaagagc acgaacacga
    55381 acacggagat cgcgagcgac gtgatgagct gcccgcgcat gtagccaccg acgatcgccc
    55441 tgagctcgat gaggatcttc gccgtcttga cgtgatgcgg ccgaggcaca acggagtaga
    55501 taaggccctt cgagcgcgtc ggatcggcga ggaaatacat cgcgaggaat agcgtcgaga
    55561 tggcgtaacc gatcaagagg agcagcttcg tcgagtagcc gacgaggatc gtcgcagcgc
    55621 gagcgccgag atcctgcacg ggcaactctt gcagtgactt gatgatcggc tcggcgccgt
    55681 ggcgtccctg gagactcttg atgacgtcgt cccgaacctt cggcgcgtta ctgatgagat
    55741 cgacaagctg cgcgacgagc ggtggcacca taagcagcac gagacctgct accgcgagac
    55801 ccgccacgac gaacacgagg accagcgccc ggccgcggcg caacccgcgc ttctcgagcc
    55861 acgccacgat cggatcgaac gtgccgacga gcacgagcgc tacgacaatc acctcgatca
    55921 cggcggcaag ctgcgcgacg agccacagtc cggcaacgat cgcgaatacc agcgcgatgg
    55981 tcttcggtgc gatctcgatg cgaagccggc gctccagcgg ttcggacatc cccgtacgtc
    56041 gtgcaatgca cagaccatcg agcaccgtgc gcgtatgcca cgcggcccgt cggtagcgcc
    56101 taacgacggc ggcggcgcag cgccaatgcg aacagcccga cgcccacgag cgacgtcgcg
    56161 cccgtcccgc gccccgtgcc acagcaaccg ccgtccgcga cctcgctgtc gtcgatcggt
    56221 acacgcagcg catcgaacat cacgtgccga tccatcgagc cggcttcgcc ggtgttgtcc
    56281 ccgagcaaaa catgctcatc gcccgccccc gcaaagtcga aatcgcccag gttgacccag
    56341 ccgctcacgc tcgtctgatc caccgtgacc gtgtctgtaa cgccagcgtg cacgatcttg
    56401 tacgtcgccg acaccgacgt gccgacttcg ccgccatcga ggtagacgtc gacgggatag
    56461 cgacccgcgg gcaccttgat gatccacatg ccgaagttcg cgggcgccgc tgcgctcgtc
    56521 gtaccggtcc actcgcggcc attcgcgccg tagccaccgg gctctctgcg ccaatagcga
    56581 ggatcgccgc ccgcgatgta gcactcgctt cgctgatcga tcacgccgcc ggcgggcact
    56641 ccggcgcagc gacccgacgt gtcccagaac tcggcgccca tctcgtcgaa cgccttgagc
    56701 gcgttgtcgc gatagccgga acgtcgcgag ctgcacgtcg cgcttgtcgt gcagcagccg
    56761 ttgtaccggc acacgatgaa gtccgcccat tcttcaagcg tcatcgtgcc ggtctgcatc
    56821 tgcacgttgt tcagccaatc catcgccgat agccaatcgg tcgcgtgctt gtcctggatc
    56881 gcgcgcacct cgacgaagtt cacggcttgc gcggtgttcc cctcgatcgt caggatcgaa
    56941 tcgccatacg tgttgatcgt gtcttgatac gtgccggagt cgaactggaa catgccgagg
    57001 ccgccctgca tcgccgagca cgggccgtcg gcagcgcccg cgatcacggg gccaccgttg
    57061 cacgacggcg aggccggccc catgcacgcg aacgtcgctt cgctctggca gtgcgcgagg
    57121 ttcgtctcgg agatcgcgac gcccgcgagc agcgatgcgt tgtagaggcc catctccgcc
    57181 gcagagtcgc gaatgagacc gaagcgctcg tgccgcaccg cggtcgtgat cgcggcttcg
    57241 tccgtcgacg tcgcaggcgc gcaggccgca agcgtagcga cgagcagtcc cccgtacttc
    57301 acccgaccat cgtggcatga aggcagggcc gcgtgggtgt cgatcaacga tggctgatct
    57361 gctcggtagt tacgtcgacg atgttcggac ggccttcggc ccagctgcgt tcggatgcga
    57421 gttgctcgtg gtcagcccaa tgcacgagga cgaagggatg cggaatcgcc gtctgcatgg
    57481 tgtacccgcc cttcgcgatg ccgaccttgc gcacgcggga cgtctgcaga tccatgatcg
    57541 agacgtcggc gtcgaaggtc atgcgagccg gccacgagcc atcttccttc gcgccaccgc
    57601 tgacgagcag cagacgatgg ccgcggatcg catcaccact cgctggcgac atcccagccg
    57661 gcagcttcgc gagcgtctgc gcgacgcccg gcgcggtcag cgcgatcagc tcgcgctcgg
    57721 atgagatcac gatcgcacca tcgagcgtcc cgaccacctc gggatagcct ccctgccaga
    57781 ccaggcgcag tgcgtcccta ctgggatcga tgtcgttgag cctaacggtg ccgtccgcac
    57841 cgcgcgtcaa taccagccac cccatgcgcg ggaccgcgag caacgccgca cgcccgagct
    57901 ccgtcacctg cttcgtcgaa ggctcgaaga tcgagacgac gttcgcattg tacgcgtcgt
    57961 agcgaaccac gcgtccgtcg cgcagcgcgt actcctggaa gtcgtggacg tgctgatacg
    58021 gcgaacgcgg gtagagcgat ggcagcagaa atgccggctg cgccggttcg ggcgtcgtcg
    58081 gctccgcaac cacgcccatg tcacagccga ggagaaacac gattagcagg gcacgcatca
    58141 tgatcaagat acgtgcgcca gcgcgcttca atgcagcggc ttggctggcc ttacagaggc
    58201 cttacatcag aagtcggtgc cgacgccgag ctgaacgatc agcgggttca cccacatgct
    58261 catcttcgcg gtgccaacct cgacggtatc gaagatcggc agctcgggcg tttgcacttg
    58321 aatgtgctcg acacgcgcgc gcgccaacat gccggcgatg aacttcacgt cgaggcgcgc
    58381 gtagacgcga ttcgtgatct tcgcgtcgat gccggtctgg agcacgacgc cgggcgccgg
    58441 ggagatcgac atcttgggct gcgagaccgc cgtcagcatc gggttcgtca ccttcgcgtc
    58501 cgtcgagaac atcacggcga cgccgccacc cgcataggga tgaatgcgcg gcgaattgcc
    58561 gaacgtgtag atcatcgtga cgaccggcgg gactgccttc gcttcgccta gctcggaacc
    58621 gagcggaccg acgcccgtcg gcaagccgag cgccatcggc gcgatcgatt cgttcgcgag
    58681 cgtaccagtc gcgcgaaact tcaccgtgaa tggcaagccg atgatcgtct cgatcgcgat
    58741 gcggccgtct ttccatggca gctgatagcc gacgatgatg ccgggaatcg tcgcggacga
    58801 gaccgtcgcg cccgagcctt cgatcggtcc gttctgcacg gcgagcgatg cgggaccgtc
    58861 gacgtcggcg agctccatcg gtcgtgattg ctcgagcggc tcgaggtgaa cgacaccgcc
    58921 gcgtacgtag agcgggtgct tgcgatcggc atgcgcgagc gtcggccgga gactgccgac
    58981 gagcgcgaga gtgacaacaa cggcggattt catagcgaca ctccacgcgg cgcgggggag
    59041 acgagctgga ggttcatctc gcccttgggg atgctcatct tgacgtggcc cgatgcgggg
    59101 ccgctgatcg agacgtcgac gggaacgtcc gccgtgttcg acagcgcgat cgagatgcgg
    59161 ccgtcgtcga ggaatgtgac cgggccttcg aggacgagcg acagctgctt gctgtgcagg
    59221 tcgtggctcg acagcgtcac gctcatgtcg ggcgcgtcca tgtagaggtc gagcgcgacc
    59281 atgatcttcg gcgtcccgcc gttgtcgatg atgtagccca tcacctggcc gccagtgcct
    59341 tgccggatgc gcatgatctg cgtgcctgtc gcggtgctga tcgtcgtgag cccgatgtcg
    59401 gcggccatcg tgatcgacgt accgaacatc gtctgcgccg acatctgcac gggaatgcag
    59461 gtcgctgccg tggcgccgcc gggaagcggg cacgtcgtcg tcgcttcgcc catgacgacc
    59521 ggcatcgcgc cgacgagata ggtgcagccc ttcttgttgt cgacggcggc gtcaccgcac
    59581 gtgttgccgt tgaacgtcgc gctgttgatc gatcccgtcg tcgacgtgat gtgcatcgcg
    59641 gcgcgattct cggtccgcat ctgctcgctc gagtcgacga cgccgttgcc gttgacgtcg
    59701 gtagccggcg ctccggtgtt catcatgtac gtgcccttcg acggcgcacc accggtgaac
    59761 ggcatcgtca gcgcggggcc tccgccatcg ccgccgccga gacccgcgag cggatcgaag
    59821 ctgagcgcgc tcgatggatc gcagagctcg cccgcgtcgc agctgtcatt ggtgcccgag
    59881 acgagcgtca gcttgtagcg cttgcccatc acccacggca cgtcggggat gaacgtcatc
    59941 gtgcgctcgc gagcgatcag cgtgcccgcg acgaccgccg tgcacgtgcc cgctgcatcg
    60001 gtttcctcga cgcggaacgt gccctggttg catgccatgc cgcgggtcac cgacgtgcgg
    60061 cgaacgggct c
//
LOCUS       DATLBZ010000466         4209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2820329, whole genome shotgun sequence.
ACCESSION   DATLBZ010000466 DATLBZ010000000
VERSION     DATLBZ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2820329"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VMZ53_23715"
     CDS             <1..504
                     /locus_tag="VMZ53_23715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31542.1"
                     /translation="ANAATNAANAASDAADSAAMAERSAQVRLADMLGNVDSIDEVRA
                     HGHTFTFLVEHADATGYEAPFAIVATTGDAGNVISVVEHARVASDMDLGGLSWLVDTM
                     QQTIAVTSLDVDDDGSVTLTTNDGARYMAIPGRGSGGSGATADDAGANIEVEARWAAE
                     FDHEPQS"
     gene            708..1250
                     /locus_tag="VMZ53_23720"
     CDS             708..1250
                     /locus_tag="VMZ53_23720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31543.1"
                     /translation="MKASKFIVLVGGILGILAFFLPLVSVERQGHKASVSAFQVMKGL
                     DQAEASVDEGVQQSAFSADTGASAKKDIGAIKGIVMAVFAPALLLVLIGGLGVARKKF
                     GRVAGTFSFLLGLLGLGIGAILKAAAEGDGGIGLTFLLLTGFAGVVGGLATLIKPERG
                     ASPAQSQFATVPAVAPRMAA"
     gene            1449..2777
                     /locus_tag="VMZ53_23725"
     CDS             1449..2777
                     /locus_tag="VMZ53_23725"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HUS31544.1"
                     /translation="MRAPLIVVLLAMIPACMVGEGGQANDDAVAGDGLRDSDVTGTSG
                     SGVGTIAARVCPNGPTVNGIDVSYYQGVIDWSAVKASGIEFAFVRLSDGAVFRDPKFD
                     VNWQGAKSAGVIRGAYQFFRPGQNVDTQADMMINAIGTYQPGDLPPVIDVEAAGGLSP
                     STVATRVKQWVTKVKGALGVDPIVYTGKYFWRDQVGGERALASNALWIAQYTSLCPDI
                     PTPWTRWTFWQHSDRGSVPGIREKVDLNKFNGTLADLASFAGGGGTPPPSFNQAPLPF
                     NWQRNFDGSYTFFAHPPAGVTRLDIRVEDYVIGDADTTKGEPVIDYTFNASRDGRDVD
                     VRGYDAQGAVIAVGNGLIDTTTSPKVFVDMTAASTYEFGLEDAPANAATVEVTADGYP
                     LLDQVSGRYKSTRGMVEYAFTQPGVRELTVTTRDAGGTLLRQDKRTFQVR"
     gene            2818..3738
                     /locus_tag="VMZ53_23730"
     CDS             2818..3738
                     /locus_tag="VMZ53_23730"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUS31545.1"
                     /translation="MAKQQQYDPRTDPDVPKGMSIELLQDLHLLTRDGQLNADARRKL
                     KQIRHLVGLLKPALDDALARSPEPSIVDCGAGKSYLGALLYELVLGPAGRGTLTAIEA
                     RPELSEQAANRAKRFGQERFKVATGTIGSFIAPPGSASSSSGSPSSSSGAASVARPNV
                     LTALHACDTATDDALVLAIANGADHVAVVPCCQAEVARQLEASKPDDAVLASLFAHPL
                     HRRELGSHLTNVIRALTLEAHGYKVTVTELVGWEHSAKNELILGKRVQRFDRRARETL
                     RTLLDRFHVEPALIRELTARNLDPRKDAST"
     gene            complement(3788..>4209)
                     /locus_tag="VMZ53_23735"
     CDS             complement(3788..>4209)
                     /locus_tag="VMZ53_23735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31546.1"
                     /translation="YLNVTVQAKKRRVVGGFIDTGSGMTILSNLAAEALGIDTSKPTD
                     YTHVGGFRRDICFAIVDIHIPGTDCFVEGFRVAVIANGAEDIPGALIGADFLQRTGAL
                     LDFRKGRHSIAGDVEGRDSAPEPFVRAKPVRVRRSRS"
BASE COUNT          771 a         1430 c         1329 g          679 t
ORIGIN      
        1 gcgaacgccg cgaccaacgc cgcgaacgcc gcgagcgacg ccgctgacag cgcagcgatg
       61 gccgagcgca gcgcgcaagt gcgcctcgcc gacatgctcg gcaacgtcga ctcgatcgac
      121 gaagtccgcg cgcacggtca cacgttcacg ttcctcgtcg agcacgccga tgcgaccggc
      181 tacgaggcgc cgtttgcgat cgtcgcgacg acgggcgacg caggaaacgt gatcagcgtc
      241 gtcgagcatg cgcgcgtcgc gagcgacatg gatctcggcg ggctgtcgtg gctcgtcgat
      301 acgatgcagc agacgatcgc ggtcacgagt ctcgacgtcg acgacgatgg cagcgtcacg
      361 ctgacgacga acgacggcgc acgctacatg gcgattcccg gccgcggctc gggcggcagt
      421 ggcgcgaccg ccgacgacgc gggcgcaaac atcgaggtcg aggcgcggtg ggccgccgag
      481 ttcgaccacg agccgcagtc gtaaaccgga cgcggcgatc tacgtagagc ccgtcgagga
      541 ttcgcggtcg cacagtattt tctcgtcgag gatcgcggac ccaaccgatc tcgacgatcg
      601 ggtgcgttca gacccgccga tgtacgccaa gtccgcgact gcaaatcgcg aacggatggc
      661 cttccgcctg cacgaggcag cctccactca acggaggcaa caaggtcatg aaggcaagca
      721 agttcatcgt tctcgtcggc ggcatcctcg gcattctcgc gttctttctc ccgctcgtat
      781 ctgtcgagcg acaaggacac aaggcaagcg tcagcgcatt tcaagtcatg aagggactcg
      841 atcaagccga ggcgtcggtc gacgagggcg tgcaacagag cgccttcagc gccgacaccg
      901 gtgcgagcgc gaagaaagac atcggcgcga tcaaaggcat cgtgatggcg gtgttcgcgc
      961 ccgcgctttt gctcgtgctc atcggcgggc tcggcgtcgc gcgcaagaag ttcggccgcg
     1021 tcgcgggaac gttctcgttc ttgctcggtc tgcttggtct cggcatcgga gcgatcttga
     1081 aggcggcggc cgaaggcgat ggcggcatcg gcctgacgtt cctcttgctc accggattcg
     1141 ccggtgtcgt cggtgggctc gcgacgctca tcaagccgga gcgcggcgca tcgccagcgc
     1201 agtcgcaatt cgcgacggtg ccagcggtcg ctcctcgcat ggccgcgtag tcacgcgttg
     1261 ccgcgctgcg tggcggctcg agttgccagt gctcgaacac gacacgctcg ccgcaatgtg
     1321 cgccacgaat tggctgggag actcgtcctc aggcaagagc cccagtcgca cgagatgacg
     1381 ccaggtcccg ggaaaccgcg acttggcgtt ttcgcgttca ggcacatgcg ttgctgattg
     1441 gttcgcgcat gcgcgctcca ctgatcgtgg tccttctcgc gatgatcccg gcgtgcatgg
     1501 ttggcgaagg cggccaagcg aacgacgacg cagtcgccgg cgacggactt cgcgattccg
     1561 atgtcaccgg cacgagcggc agtggcgtcg gcacgatcgc cgcgcgcgtc tgcccgaatg
     1621 gcccgaccgt caacggcatc gacgtctcgt actaccaagg cgtgatcgat tggtccgccg
     1681 tcaaagcgag cggcatcgag ttcgcgttcg tgcgcctctc cgatggcgcg gtctttcgcg
     1741 atccgaagtt cgacgtgaac tggcaaggcg caaagtccgc aggcgtgatc cgcggcgcgt
     1801 atcagttctt ccgtccaggt cagaacgtcg acacgcaagc cgacatgatg atcaacgcga
     1861 tcggcacgta tcagcctggc gacctaccgc cggtcatcga cgtcgaagcc gccggcggcc
     1921 tctcgccatc aaccgtcgcg acgcgcgtga agcagtgggt gacgaaagtg aagggcgcgc
     1981 tcggcgtcga tccgatcgtc tacacgggta agtacttctg gcgcgatcaa gtcggtggcg
     2041 agcgcgcgct cgcgtcgaac gcgctgtgga tcgcgcagta cacgtccctc tgcccggaca
     2101 ttccgacgcc ctggacgcgc tggacgttct ggcagcacag cgatcgcggc agcgttcccg
     2161 gaatccgcga gaaggtcgac ctcaacaagt tcaacggcac gctcgccgat ctcgcgtcgt
     2221 tcgcaggagg cggaggcaca ccgccgccgt cgttcaacca agcgcccctg cccttcaact
     2281 ggcaacggaa cttcgacggc tcctacacgt tcttcgcgca tccgcccgcg ggcgtcacgc
     2341 gcctcgacat ccgcgtcgag gactacgtca tcggcgacgc cgacacgacg aagggcgagc
     2401 ccgtcatcga ctacacgttc aatgcctcgc gagacggccg cgatgtcgat gtccgcggct
     2461 acgacgcgca aggcgcagtg atcgcagttg gcaacggtct gatcgacacg acgacgtcgc
     2521 cgaaagtctt cgtcgacatg accgcagcgt cgacgtacga attcggcctc gaagacgcgc
     2581 ccgccaacgc cgcaacggtc gaggtcacgg ccgacggcta cccgctgctc gaccaagtaa
     2641 gcggccgcta caaatcgacg cgcggcatgg tcgaatatgc gttcacacaa cccggcgttc
     2701 gcgagctcac tgtcacgacg cgcgacgccg gcggcaccct cctgcgccaa gacaagcgca
     2761 ccttccaagt ccgttaacag cgctagcgcc catagccgct cggggtacag tgcggcgatg
     2821 gcgaagcagc agcagtacga tccacgtacg gatcccgacg tgcccaaagg catgtcgatc
     2881 gagctgctcc aagacctgca cctgctcacg cgcgacggcc agctcaacgc tgatgcgcgc
     2941 cgcaagctca agcagatccg ccacctcgtc ggcctgctca agcccgccct cgacgacgca
     3001 ctcgcgcgca gccccgagcc atcgatcgtc gattgcggcg cgggcaagag ctacctcggc
     3061 gcgctgctct acgagctcgt gctaggcccc gccgggcgcg gcacgttgac cgcgatcgag
     3121 gcgcgcccgg agctctccga gcaagcagca aatcgcgcga agcgcttcgg ccaagagcga
     3181 ttcaaggttg cgactggcac gatcggctcg ttcatcgcgc cgcccggctc cgcgagctcg
     3241 tcgtcgggct cgccaagctc gtcgtcgggc gcggcgtcgg tcgcgaggcc gaacgtgctc
     3301 accgcgctgc acgcgtgcga caccgcgacg gacgacgcac tcgtcctcgc catcgcgaac
     3361 ggcgccgatc acgtggcggt cgtgccgtgc tgccaagcgg aagtcgcgcg tcaactcgag
     3421 gcgtcgaagc ccgacgatgc ggtgctcgct tcgctgttcg cgcacccgct gcatcgtcgc
     3481 gagctcggct cgcacctcac caacgtcatt cgcgcgctca cgctagaggc gcacggctac
     3541 aaggtcaccg tcaccgagct cgtcggctgg gaacactccg cgaagaacga gctgatcctc
     3601 ggcaagcgcg tgcagcgctt cgatcgccgc gcgcgagaga cgcttcgcac cctgctcgac
     3661 cggtttcatg tcgagcctgc gctgattcgt gagctcacgg cgcgcaacct cgacccgcgt
     3721 aaagacgcat cgacctgacg gtacaaccaa gttcttcgac ggatgcgcag tgactcggcc
     3781 gcagccgcta cgacctgctc cgccgcacgc gaaccggctt tgcacgaacg aaaggttccg
     3841 gcgccgagtc tctgccttca acatcaccgg caatcgaatg cctacccttc cggaaatcga
     3901 gcaaggcacc ggtgcgctga agaaagtccg caccgatgag tgcgccagga atgtcctccg
     3961 ccccgttcgc gatcacggcg acgcgaaatc cttcgacgaa gcagtcggtg cccgggatgt
     4021 gtatatcgac gatcgcgaaa caaatgtcgc gacgaaagcc acccacgtgc gtgtagtcgg
     4081 tgggctttga cgtgtcgatg cccaacgcct ccgcggcgag attcgacagg atcgtcatcc
     4141 cggatcctgt gtcgatgaag ccgccaacta ctcggcgttt tttcgcttgc accgtcacgt
     4201 ttaggtaca
//
LOCUS       DATLBZ010000467         6060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5798658, whole genome shotgun sequence.
ACCESSION   DATLBZ010000467 DATLBZ010000000
VERSION     DATLBZ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5798658"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VMZ53_23740"
     CDS             <1..1418
                     /locus_tag="VMZ53_23740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31547.1"
                     /translation="PLDWYLATLVPEATLFGTMQRLGRQSIIVSGAALAIAMGVALMF
                     AWNILRMRRTVAVAREQARSAEARAAQLGSYRLVEKLGAGGMGEVWRAEHQLLARKAA
                     IKLVRKDVLRDRQHAELIQERFRREAQPLASMRSRHTIELYDYGTTDDGTFFYVMELL
                     DGVDLDRLVRDQGPLPPARVIKLLVQACQSLAEAHDNGLLHRDIKPANLFVCRCADEV
                     DVLKVLDFGIVHTIAEPMPDPMDVVSLPVPQSPEPPPSRERIGAPSSGRLTAIGSVIG
                     TPGYIAPEQALVGSTVDGRADLYALGCVAWWLLSGTEVFRRMDTNESMRAHVTEAVPP
                     LRPLVKGWMPNGLEQLVVSMLAKNPDDRPADMRALIAQLRAIEIPREHAWTDAAALAW
                     WNQTRPNTKVATSDAATAAMSPMLIGASMESTREKSSLIHSAPPTMPAAQIANAGPPT
                     MPADAKTGEMPRSFGRPPTID"
     gene            1513..3114
                     /locus_tag="VMZ53_23745"
     CDS             1513..3114
                     /locus_tag="VMZ53_23745"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HUS31548.1"
                     /translation="MRKTLASVLLGTTLLTASCSNKSADMRSSAVKEESMSVDRPYDT
                     TTAQNVASGSAYAGAGVASPAPQMYDKAKLADGKEGGEAYHDWGKNPWIDASKDHLST
                     FAADVDTASYSIARRKLEEGALPPQAAVRVEEFVNYFKYSFPQPSGNSPFSVVMEAAP
                     SPMQAGRTIVRVGVATKAKSTMERKPAHLVFLVDVSGSMDSPDKLGLAKQALEILTRG
                     LKEEDSVSLVTYAGSTRVVLEPTNNHDEIIAALDDLKAAGSTGMASGIDLAYRMAMQN
                     MKPNTINRVVILSDGDANVGPHTHDEMLKLISGRAKEGVTLSTIGFGTGNYKDELMEQ
                     LADKGNGNNFYIDSMDQAKRVFQQDLTANLEVVAKDVKLQVDFDKDIVARYRLVGYEN
                     RDVKDDDFRDDKVDAGEIGAGHQVTALYEVELTAKGKQAHAPIGAIRIRHKAPDGEKA
                     TEAAFPMVGGPAASFANSSADFRFAFAAAAFADVLRGGQDAEHWSLASIRDMAKNAAG
                     DNADRKELVTLIEKAMQISGRTASR"
     gene            3145..3915
                     /locus_tag="VMZ53_23750"
     CDS             3145..3915
                     /locus_tag="VMZ53_23750"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUS31549.1"
                     /translation="MRVAVVVLVVLLRVATTAHAEPWHKGRYGTNRIVHLSIAVGGSL
                     LYTASGPLEDRWMATECRWCTPTALDVATRDALRWNDVSTAKTLSDVTAYGITPAFAM
                     SFVLAGTLADPTTAALIDDLTPILEATIFARFATSALKLAIGRQRPDAHFSGATDEEN
                     VSFPSGHTSGAMSVAISAAVVARMRGYRSEPYLWIGGSLIAMSVGYFRIAGDRHYVTD
                     VLAGAAVGATAGLTVPYLMKRNVSVVPTRDGIAVAGSW"
     gene            3932..>6060
                     /locus_tag="VMZ53_23755"
     CDS             3932..>6060
                     /locus_tag="VMZ53_23755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31550.1"
                     /translation="MRSVVVLLLVIGVAGVAGATPPEPKGPHPRMLLDEALKRDLRTY
                     GKLPVGPVAGAIKLCDEARTTDEHVRALYQGAEWAKVLQACLVAWAATDDKNHANTAI
                     KYFTALIDDLDNLGDGKGGDAAATRDHGYAIRNLGPYTALAYDWLNKQMPEPLKARAR
                     ARWKAWLDWYAEKGYRARIAGSNYQAGYLIAATTIAIAEAGEAGTDGTELWRLVADEL
                     WGKDMAHALTTDGVLAGGDWPEGWQYGPLSVAEYALGARIMKGAGVDVPGMQPWLSSL
                     LRRHVYALSPGEQTYAGGDTEDEQANLSPHVLTLAAVALGDASPQDRTWARGELARLR
                     ITDRDYFLYTSLASIGEGKPDAPPRAAWPTWYVSANTGTLYARTRWDDTAIWFVTECH
                     ATIETDHRHPNAGTFSLSRGKDDVIVDPTPYGSQSTLTSNAPGIDSPQLPKDYLPSQG
                     MWSEKTGYDFITQRASGIVAARCDYSDQFKFQDKPSDVPAALRDWVLIPSQDGRDAAL
                     LVIDRADTTSAERNLDLRFRTPGKLELADKNASATVGSTKLTIAQVTATGGTLALGAN
                     RHKDCYKFTGPKGQCDAARFPITDYRVRIPGPRPTAVHLISAAAKTAATAQPLTGKDW
                     AGSAIKGVRDAVVVWRTSGSGGFSYTAPANAMHLVLDAPEGGTLTATAAGGNCNVKVD
                     GAGAPGKPLVAMLDAQCKVTPDPEAAAA"
BASE COUNT         1105 a         2093 c         1928 g          934 t
ORIGIN      
        1 cgccgctcga ctggtacctc gcgacgctcg ttcccgaggc gacgctgttc ggcacgatgc
       61 agcggctcgg gcggcagtcg atcatcgtgt cgggcgctgc gctcgcgatc gcgatgggcg
      121 tcgccctgat gttcgcgtgg aacatcctcc gcatgcgccg caccgtcgca gttgcgcgcg
      181 agcaggcacg gtctgccgag gcacgcgcgg cgcaactcgg cagctatcga ctcgtcgaga
      241 agctcggcgc cggcggcatg ggcgaggtgt ggcgcgccga gcaccagctc ctcgcgcgaa
      301 aggccgcgat caagctcgtc cgcaaggacg tcctgcgcga tcgccagcat gccgagctga
      361 tccaggagcg gttccgccgc gaggcacagc cgctcgcctc gatgcgctcg cgccacacga
      421 tcgagctcta cgactacggc acgaccgacg acggaacgtt cttctacgtg atggagctcc
      481 tcgacggcgt cgacctcgat cggctcgtgc gcgatcaagg accgctgccg cctgcgcgcg
      541 tgatcaagct gctcgtgcaa gcctgtcagt cgctcgccga agcgcacgac aacggcctgc
      601 tccaccgcga tatcaagccg gcaaacctgt tcgtctgccg ctgcgccgac gaggtcgacg
      661 tcctcaaagt tctcgacttc ggcatcgtcc acaccatcgc ggagccgatg cccgatccga
      721 tggatgtcgt gtcgctgccc gtgccgcagt cacccgagcc gccgccatcg cgtgagcgga
      781 tcggtgcacc gtcgtcggga cgcctcacgg cgatcggttc ggtgatcggc acacccggct
      841 acatcgcgcc ggagcaagcg cttgtcggct cgacggtcga tggtcgcgcg gatctctacg
      901 cgctcggctg cgtcgcgtgg tggctgctct cgggcacaga agtgttccgc cgcatggata
      961 cgaacgagtc gatgcgcgcg cacgtcacgg aagccgtacc gccgttgcgg ccgctcgtga
     1021 agggctggat gccgaacggt ctcgagcagc tggtcgtctc gatgctcgca aagaatcccg
     1081 acgatcgacc ggccgacatg cgcgcgctga tcgcgcagct gcgtgcgatc gagatcccgc
     1141 gcgagcacgc atggaccgat gccgcggcgc tcgcgtggtg gaaccaaacg cggccgaaca
     1201 cgaaggtcgc aacctcggat gcggcaacgg cagcgatgtc cccgatgctg attggcgcga
     1261 gcatggagtc gacgcgcgaa aagagctcgc tgatccacag cgcgccaccg acgatgccgg
     1321 ccgcgcaaat cgcaaacgcg gggccaccga cgatgccggc cgacgcaaag accggcgaga
     1381 tgccgcggtc cttcggccgc ccgccgacaa tcgactaatc ccgatccttg ggcggaaagt
     1441 tgtcgtcggg gcgcgacaag atttcacgag atcgtgcaga ccggacttcg caccggcgcg
     1501 tcgcaccaaa gcatgcgcaa gaccctcgcg tccgtcctcc tcggcaccac cctcctcacc
     1561 gctagctgct cgaacaagtc agcagatatg cggtcttccg ccgtgaagga agaatcgatg
     1621 tcggtggacc gtccttatga caccacgacc gcccagaacg tcgcttcggg ctccgcctac
     1681 gcaggcgccg gtgtcgcgtc tcccgcacca cagatgtacg acaaggcgaa gctcgccgac
     1741 ggcaaggagg gcggcgaggc ctaccacgac tggggcaaga acccgtggat cgacgcttcg
     1801 aaggaccacc tctctacgtt cgccgcggat gtcgatacgg cgtcgtattc gatcgcgcgt
     1861 cgtaagctcg aagaaggcgc gctgccgccg caggccgctg ttcgcgtcga ggagttcgtc
     1921 aactacttca agtacagctt tccgcagccg agcggaaact cgccgttctc ggtcgtgatg
     1981 gaagcagcgc cgtcgccgat gcaggccggt cgcacgatcg tgcgcgtcgg cgtcgcgaca
     2041 aaggcgaagt cgacgatgga gcgcaagcct gcgcatctcg tgttcctcgt cgacgtctcg
     2101 ggatcgatgg acagccccga caagctcggc ctcgcaaagc aagcgctcga gatcctgacg
     2161 cgcggcctca aagaagaaga cagcgtctcg cttgtcacgt acgcgggcag cacgcgcgtc
     2221 gtcctcgagc cgacgaacaa ccacgacgag atcatcgctg cgctcgacga cctcaaggcc
     2281 gccggctcga cgggcatggc gtcgggcatc gacctcgcct accgcatggc gatgcagaac
     2341 atgaagccga acacgatcaa tcgcgtcgtg atcctcagtg acggcgatgc gaacgtcggc
     2401 ccgcacaccc acgacgagat gctgaagctc atctcgggcc gcgccaagga gggcgtcacg
     2461 ctctcgacga tcggctttgg cacgggcaac tacaaggacg agctcatgga gcagctcgcc
     2521 gacaagggca acggcaacaa cttctacatc gactcgatgg atcaagcgaa gcgcgtcttc
     2581 cagcaggatc tcaccgcgaa cctcgaggtc gttgcgaagg acgtcaagct gcaggtcgac
     2641 ttcgacaagg acatcgtcgc gcgctatcgc ctcgtcggtt acgagaaccg cgacgtcaaa
     2701 gacgacgact tccgcgacga caaggtcgat gccggcgaga tcggtgcagg ccatcaagtg
     2761 actgcgctct acgaagtgga gctcacggcg aagggcaagc aagcgcacgc tccgatcggc
     2821 gcgatccgca tccgtcacaa ggcgcccgac ggtgagaagg caaccgaagc ggcgttcccg
     2881 atggtcggcg gcccggcggc gtcgtttgcg aattcgtcgg cagacttccg gttcgcgttc
     2941 gctgcggccg catttgccga cgtgctgcgc ggtggccaag atgccgagca ttggtcgctc
     3001 gcgtcgatcc gcgacatggc gaagaacgcc gccggtgaca acgcggaccg caaggagctg
     3061 gtcacgttga tcgagaaggc gatgcagatc agtggtcgca ccgcatctcg ctgaggttct
     3121 gggagcggga tatgctccct gcggatgcgt gtcgcggtcg tcgttctcgt cgttctcctt
     3181 cgggtcgcga cgaccgcgca cgctgagccg tggcacaaag gccgctacgg tacgaatcgc
     3241 atcgtccatt tgtcgatcgc cgtcggtggc tcgctgctct acaccgcgag tgggccgctc
     3301 gaagatcgct ggatggcgac ggagtgtcgc tggtgtacgc cgacagcgct cgacgtcgca
     3361 acgcgcgacg cgctccgctg gaacgatgtc tcgacggcga agacgctgag cgacgtcacc
     3421 gcgtacggaa tcacgccggc atttgcgatg agcttcgtgc tcgctggcac gctcgccgat
     3481 ccgaccacgg ccgcgctcat cgacgacctc acgccgattc tcgaagcgac gatatttgcg
     3541 cggttcgcga ctagcgcgct caagctcgcg atcggccggc agcgccccga tgcccacttc
     3601 tccggcgcca ccgacgagga gaacgtctcc tttccgtcgg gccacacgtc aggcgcaatg
     3661 tcggtcgcga tcagtgccgc tgtcgtcgca cgcatgcgcg gctatcgcag cgagccgtac
     3721 ctatggatcg gcggctcgct catcgcgatg tccgtgggct acttccgcat tgcgggcgat
     3781 cgccactacg tcaccgatgt gctcgcgggc gccgcggttg gagctaccgc gggtctcacg
     3841 gtgccctatc tcatgaaacg gaatgtttcc gtcgtaccaa cgcgcgatgg aatcgcggtt
     3901 gcgggctcgt ggtgatgcta cggtttgcgc cgtgcgaagc gtcgttgtcc tgctgttggt
     3961 gatcggtgtc gcgggcgttg caggcgcgac gcctcccgag ccgaaaggtc cgcatccgcg
     4021 gatgctgctc gacgaagcgc tgaagcgcga cctccgcacg tacggcaagc tgcccgtcgg
     4081 ccccgtcgcg ggtgcgatca agctctgcga cgaagctcgc accaccgacg agcacgtgcg
     4141 cgctctctac caaggcgccg agtgggccaa ggtcctgcag gcctgcctcg tcgcgtgggc
     4201 tgcgaccgac gacaagaatc acgcgaacac cgcgatcaaa tacttcaccg cgctcatcga
     4261 cgatctcgac aacctcggtg acggcaaagg cggcgacgca gccgcgaccc gcgatcacgg
     4321 ctacgcgatc cgcaacctcg gtccatacac ggcgctcgcg tacgactggc tgaacaagca
     4381 gatgcccgag ccgctgaagg cccgcgcacg cgcccgctgg aaagcgtggc tcgattggta
     4441 cgcagagaaa ggctaccgcg cgcgtatcgc cggctcgaac tatcaggccg gctacctcat
     4501 cgcagcgaca acgatcgcga tcgccgaagc gggcgaagcc ggcaccgacg gaaccgagct
     4561 ctggcgtcta gtcgccgacg agctctgggg caaggacatg gcccacgcgc tgacgaccga
     4621 cggcgtgctc gctggcggcg actggcccga aggctggcaa tacggcccac tgtccgtcgc
     4681 tgaatacgcg ctcggcgctc gcatcatgaa gggcgccggc gtcgacgtgc ccggcatgca
     4741 gccatggctc tcgtcgctgc tgcgccgtca cgtgtacgcg ctctcgcccg gcgagcaaac
     4801 ctatgcaggc ggcgacaccg aagacgaaca agcgaacctt tcgccgcacg tgctcacact
     4861 cgcagccgtc gcgctcggcg atgcgtcgcc gcaagatcga acgtgggccc gcggcgagct
     4921 cgcgcgcctg cgcatcaccg atcgcgatta cttcctctac acgtcgctcg catcgatcgg
     4981 cgaaggcaag ccggacgccc caccccgcgc agcgtggccg acgtggtacg tcagcgcgaa
     5041 caccggcacg ctctacgcgc gcacgcgctg ggacgacacc gcgatctggt ttgtcaccga
     5101 gtgccacgcg acgatcgaga ccgatcaccg ccaccccaac gccgggacgt tctcgctctc
     5161 gcgcggcaag gacgacgtca tcgtcgatcc aactccgtac ggctcgcagt cgacgctgac
     5221 gtccaacgca ccgggcatcg acagcccgca gctgcccaag gactacctgc cgagccaagg
     5281 catgtggtcg gagaagaccg gctacgactt catcacgcag cgcgcatccg gcatcgtcgc
     5341 ggctcgctgc gactactccg atcaattcaa gttccaagac aaaccgtctg acgtgcccgc
     5401 ggcgctccgc gactgggtac tcatcccttc gcaagatggt cgcgatgccg cgctgctcgt
     5461 catcgatcgc gccgacacga cgagcgccga gcgaaatctc gaccttcgct tccgcacgcc
     5521 gggcaagctc gagctcgccg acaagaatgc gtccgccacc gtcggttcga cgaagctgac
     5581 gatcgcacag gtcaccgcga ccggcggcac cctcgcgctc ggcgcgaatc gccacaaaga
     5641 ctgctacaag ttcacgggcc cgaaaggcca atgcgacgcc gcgcgcttcc cgatcaccga
     5701 ctaccgcgtg cgcatccccg gcccccgccc cacggccgta catttgatct ccgccgctgc
     5761 gaaaaccgcc gccaccgcgc agccgctcac cggcaaagac tgggcaggca gcgcgatcaa
     5821 aggtgtgcgc gatgccgtcg tcgtgtggcg aacctcgggc tccggcggct tcagctacac
     5881 cgcgccagcc aacgcgatgc accttgtgct cgatgcgccc gaaggcggca cgctcacggc
     5941 aacggccgca ggcggcaact gcaacgtgaa ggtcgatggt gcgggcgcgc cgggcaagcc
     6001 gctcgtcgcg atgctcgacg cacaatgcaa ggtgacgccg gatcccgaag cggcggctgc
//
LOCUS       DATLBZ010000468        12682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9529212, whole genome shotgun sequence.
ACCESSION   DATLBZ010000468 DATLBZ010000000
VERSION     DATLBZ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12682
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9529212"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1155
                     /locus_tag="VMZ53_23760"
     CDS             211..1155
                     /locus_tag="VMZ53_23760"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HUS31551.1"
                     /translation="MLRARALEERLITMYKQGDGYFWIGGPGEEAFNVPLGLLVDKGQ
                     GVDHDYLHLHYRSSGTLLAMGADPVDSLRQMKNVATDPYSRGRNFAGHFSVRKWNVVP
                     VTSPIEVQFSIAIGTARAQRAAKGITIVQGGDAGTAEGDFATALVWASRKGCELPLLM
                     IVTNNRFGISTAYEGQHGYDQISERGKAFGMPTAVIDGNDPEQSYLAIEKALHYVRTE
                     RKPYLLEAMVSRLRGHSSASGANVVKGEIDCLETFETKLLERKLLTRAQIDQLRTGYV
                     DELAEAAKRVRREPQPDPSSIHDYIFADKNHVAGEGER"
     gene            1158..2213
                     /locus_tag="VMZ53_23765"
     CDS             1158..2213
                     /locus_tag="VMZ53_23765"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HUS31552.1"
                     /translation="MATMIQAIRLALHVGETKLGVTDIFGEDVGPPLGGVFTGTQGLT
                     TAWNTPLDERGIVGTAIGLALAGQKPVAEIQFCDYAFNTIDLLKIAGNTYWTSGGDWN
                     VPMVLMTPVGAGIRGSVYHSHSFDATATRIPGWKIVMPSTPLDAYGLLLSAIKDPNPV
                     MFLMPKALLRIKSDALIPGEPEDEKQLSSMIDAPLGDRTAWKPTWPAVEEVYIPIGEA
                     RVAREGAHVTVLTYGRNVPMCLTAAEELAPDGIDVEIIDLRSLHPYDWPAIKASVQKT
                     NRVLCVNEDTEVTNFGEHLVRRIVEELFYELHAPPKLLAGAHIPGIGLADNLERASVP
                     HKDGIKAALVELARHEP"
     gene            2216..4723
                     /locus_tag="VMZ53_23770"
     CDS             2216..4723
                     /locus_tag="VMZ53_23770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31553.1"
                     /translation="MGELSRWLLVVPALLVAGCLNSASVDCGDGRVCPSGTMCHPLGC
                     VAPSQLETCHGIADRVACTLPGSMRGVCKSEVCIAEGCGDGFALGGEECDGVDFAFAD
                     CKALGYHDATPLETCRGDCTFDRSVCGSRCGDGVLDPAFEDCDASALEQVADCKSLDY
                     YNAGTVTCTSFCTFDSTQCTGFCGDGVVNGGEFCDGSASDAGCAAFGYDAGRIGCASG
                     ACAPLFGSCVQLGWQPIVTEPDVYVTAFWGAASDDIWAVGGDLAILHFNGQGWTKVSD
                     VFGGDSYNSIWGTAANDVWVVGGGIGSKLAHLQNGVFVAVTSPTTQPLFGVWGSSPAN
                     YWAVGGAGTILHYDGQSWTVAPTLTNRALRAVWGNGPDNVWAVGDGGVVLHRTSSGWS
                     VHTASMTNISSLNGVWGDSTGTVWLAGQTGDNYSVLLRGPSSWSDMGVRFFGALTSVW
                     GSGPKDVWFVGGDNEDIDTAQIFHYDGARITEHETPAGLNSLSYVWGTGPNDVWSYAI
                     GEQFTHNEGTGWRRPQTGDVAPAFFHPWDIYGASPTSIWIVGDNFDDGITVRYNGTNL
                     VAVTPTFPDLLYAVTGSGPNDVWAVGQSGIVVHSTNGSSWTEITPRPFSTDLFAAWSP
                     GPNKLIACGNGVVIEFDGQDWSGHSIDGSCSGIWGSAPNDVWAVGSHIWHYDGTTWTQ
                     LPSSPAPDGLFEIWGSGKNDVWAAGRNGAVVHYDGATWTSIDTVAVGLLTTLWGTAPN
                     DIWMAGQLGAVTTVYHYDGIGWLEVDVAASSDWISAIGGTARTILFGGRNANNASFVR
                     VLDRTSPWSCSTMESACNDAVDNDCDGKIDDEDVDCP"
     gene            4797..5381
                     /locus_tag="VMZ53_23775"
     CDS             4797..5381
                     /locus_tag="VMZ53_23775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31554.1"
                     /translation="MKRLWWTLVIVGCASASRETTMPRANIGTKYARQTGYFASCGAR
                     CDEETTSGGIRHDSILDEVTLAKVDAQETCFDVTIRAEESKDEPFSEIHPQCSIDGGS
                     QQSAIESELVSVFDYNYTGQQNVAVVEGVAASQYLGMAVSKPADMVFRVIERKGMICC
                     GRPASTSAALEFRNAHYDYGASKGRLQMRWKLTN"
     gene            5381..6532
                     /locus_tag="VMZ53_23780"
     CDS             5381..6532
                     /locus_tag="VMZ53_23780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31555.1"
                     /translation="MQLTHRRWVARAVAALVACSVVRLAVPWWIGRDGERWYDGDAEL
                     QRGLANELVAFEHSDDVDAARPPRDRFTGEWALVTHQMIALGLAQLCLAHPEWKPALA
                     PVVTTAATKSFRREMRGFGTQAWRGEDAMASLATDHGHAYLAYAALALGMARLVDPAF
                     PPALAREHDALIAAYERRLLASPTGLIETYPGEAYPTDVAAVAAAIAIHGRATHTSHA
                     AVLEHWAQRVRTVQIDHASGLVLQRMDATTGKPHDAPRGSGTALAAYFAGFADRDIAK
                     LLGQGVLRHESTRFGFGGIREYAAGHSGAGDVDSGPVLLGISVSATGFALAPARAFHD
                     HDAFISLYRTVALFGMPADSGDRHRFAIGGPIGNALLLALLTSGPELAP"
     gene            6529..6798
                     /locus_tag="VMZ53_23785"
     CDS             6529..6798
                     /locus_tag="VMZ53_23785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31556.1"
                     /translation="MKRAWWPEAKPFVGPAAIVVIYAIVRFAFITVAAGQGVLSPSGN
                     VDSAVAALALATFVLRFLALFVVPMTVIYRLVMRVSRRWTAPTSR"
     gene            6833..8341
                     /locus_tag="VMZ53_23790"
     CDS             6833..8341
                     /locus_tag="VMZ53_23790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31557.1"
                     /translation="MTLSVDPPSMKHRDIDGVGLGLVATMDLLPVGVAHFAPDGTFLF
                     VNEALSEILGFTRQELLTKSFQQISFPDDLHECLRLNAELATGAIPRYVHEKRFIRGD
                     GSIVWVRVTVTAARNRPAPAVAFFVAVAEDITAQHLAEEARRTAEARLQLALRAADIG
                     IFRWDLVADRLEADENLLQLFALARDESTKTQAFLSRIHPDDRPRLEEATARSMHGAE
                     IDEEFRIILPDGTERWIRDRARTLLADDGSPICVTGACADVTARRQLDQTVRHALQTA
                     ERAVSARDEAIAILAHDLRNPLGNISLASAEVPSDEAARVRRFAIIRRAAIAMERLIS
                     DLLDIARIDAGTFAVEKAPTDIRSLLDETVELFAEPAANAGVQLRCTVDDGVPSSALA
                     DRERLRQVLSNLVGNALKFTPRGRHVHIGASADNAGGVLFTVRDEGCGIPSEQLAHVF
                     DRFWQANRGKRSGAGLGLAIVKGIIDAHGGSLALESEVGAGTKVMFSVESRG"
     gene            8474..8719
                     /locus_tag="VMZ53_23795"
     CDS             8474..8719
                     /locus_tag="VMZ53_23795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31558.1"
                     /translation="MQDIKLEDLVNVTGGAGVLDNMGTGASNLSNRWANNTANTLGRN
                     SFFGGVGAAAAGFAGGIVGGLQGFGHTLTEKVPLPPK"
     gene            9180..11387
                     /locus_tag="VMZ53_23800"
     CDS             9180..11387
                     /locus_tag="VMZ53_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUS31559.1"
                     /translation="MERVAHRICPLCEATCGLELAVEGDKVTSIRGHAADVFSQGFIC
                     PKGAALAELHEDPDRLRAPLVKRDGVHVEVSWDEAFAEIERRLPPILAAHGRDAAALS
                     LGNPSAHRYGLLLYGARLARAIATTNVFSASTLDQMPKQLACGMMFGHWLSVPVPDLD
                     RSQLLVVLGANPVVSNGSMWTVPDFRGKAKAMQARGGRLVVIDPRRTETAELADQHIF
                     IRPGGDVFLLLGIVHVLFAEKLVRLGRLADHVDGVDQIERAVADLSPEAVASRCGIEP
                     SVIRDLARALATTESAALYGRIGTCTQEYGTIASWLVDVINVLTGHLDAPGGAMFPKA
                     AAFAANTIGSPGKGKGIAIGRRKSRVSGAPEVFGELPMICLAEEIETPGEGQVRALIT
                     IASNPALSAPNGPRLAAAFSSLELMVSVDIYLNETTRHADVILPGLSALEESHYDVAF
                     PQLSCRNAARYSPAIFAPPAGALAEWQILLRLLAIATGRGANADINGLDDELTLADVQ
                     RVAGAQAPLLLQQLGDLKGPERLLDLALRGGPYGDRFGFVPDGLNLARIKAAPAGIDL
                     GALQPRIPEMLRTPSGKIELAPPLFIDDLGRVHADLARPAPDLVIVGRRQLRSNNSWM
                     HNLPMLAKGPFRCVALVHPADAERLALTNGGRARIANASRTIDVTVEVSDEMMPGVVS
                     LPHGWGHDQPGTKLTVAAERAGANLNAIIDDTRRDPLSGNAVLSGIPITMSPL"
     gene            11585..11827
                     /locus_tag="VMZ53_23805"
     CDS             11585..11827
                     /locus_tag="VMZ53_23805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31560.1"
                     /translation="MHDDSIQGALESYVQTQSDFADSADDNPSVGTFVEWPPKPPPPA
                     ALASIVHSNARIELPPAVDEIESIVVLDADDVDDVP"
     gene            11906..>12682
                     /locus_tag="VMZ53_23810"
     CDS             11906..>12682
                     /locus_tag="VMZ53_23810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS31561.1"
                     /translation="MSPNPDPRRWWALFLLCTAQFVVILDTSIVGIALPALQRALDFD
                     AAGLQWIFNAYVVAFGGLLLLGGRLADIFGPRRLFATGFGILTGASLLAGLATTGTTL
                     LIARALQGAGAALIAPAALSMVMGLFAARPTELRKALGFWGASAAAGGTAGVFLGGVI
                     TEWLSWRWTFLVNVPVGVLVLLATNAWLPRGSRQQGSVDVLGASTITGGLALTVYTIV
                     TANAASSARTVILLGLSAALFLAFVLIQRVRTSPLVPFSIF"
BASE COUNT         2201 a         4307 c         4006 g         2168 t
ORIGIN      
        1 aacgcgctcg acaacgccga cagccacgag tacttcgcag agaacacccc ggcgcagccc
       61 taaccagcgc gcccagacgg ttggggcctc ggccaattcc gagtaaaact cgcggtagtc
      121 gtgacttcaa gtgcatcgcg agctggtctg acctcggtga cggatacgcc gaggttgccg
      181 gccgacaaat gtcttcagat ctatgacctc atgctgcgcg cccgcgcact cgaggagcgc
      241 ttgatcacca tgtacaagca gggtgacggg tacttctgga ttggcgggcc cggcgaagag
      301 gcgttcaacg tcccgctggg actgctcgtc gataaagggc agggcgtcga ccacgactac
      361 ctgcatctgc actatcggtc gagcggcact ctcttggcga tgggcgcgga ccccgtcgat
      421 tcgctgcgcc agatgaagaa cgtcgcgacc gatccgtact cgcgcgggcg caacttcgcg
      481 ggtcacttct cggtccgcaa gtggaacgtc gtgcccgtga cgtcgcccat cgaggtgcag
      541 ttctcgatcg cgatcggcac cgcgcgcgcg caacgggccg ctaagggcat cacgatcgtg
      601 cagggcggcg acgctggcac ggcagaaggc gacttcgcga ccgcgctcgt ctgggcatcg
      661 cgcaaaggct gcgagctgcc gctgctgatg atcgtcacga acaatcgctt cggcatctcg
      721 accgcgtacg aaggacagca cggctacgac cagatctcgg agcgcggcaa ggcgttcggt
      781 atgccgacgg ctgtcatcga cggtaacgat ccagagcaga gctacctcgc gatcgagaag
      841 gcgctgcact acgtccgcac cgagcgtaag ccatacctgc tcgaggcgat ggtctcgcga
      901 ctgcgcggtc actcatcggc cagtggcgcg aacgtcgtca aaggcgagat cgattgtctc
      961 gaaacgttcg agaccaagct cctcgagcga aagctgctga cccgcgcgca gatcgatcag
     1021 ctgcgcaccg gctacgtcga cgagcttgcc gaagctgcga agcgcgtgcg tcgcgaaccg
     1081 cagcctgatc cgtcgtcgat ccacgactac atcttcgcgg acaagaacca cgtcgccggc
     1141 gaaggcgagc gctagccatg gcgacgatga tccaagcgat tcgcctcgcg ctgcacgtcg
     1201 gcgagacgaa gctcggcgtg acggacatct tcggcgagga cgtcggtcct ccgctcggtg
     1261 gcgtcttcac cggcacgcaa ggcctgacga ccgcgtggaa cacaccgctc gacgagcgcg
     1321 gcatcgtcgg aaccgcgatc ggtctcgcgc tcgcggggca aaagccggtc gccgagatcc
     1381 agttctgcga ctacgcgttc aacacgatcg atctcctcaa gatcgccggc aacacctact
     1441 ggaccagcgg cggcgactgg aatgtcccca tggtgctcat gacgcccgtc ggggcaggca
     1501 tccgcggcag cgtctaccac tcgcactcgt tcgatgcgac tgcgacgcgg attccgggct
     1561 ggaagatcgt gatgccgtcg acgccgctcg atgcgtacgg cctcctgctg tcggcgatca
     1621 aagatccgaa tccagtcatg ttcctcatgc cgaaggcgct gctccgcatc aagtcggacg
     1681 cgctcatccc gggcgagccc gaagacgaaa agcagctctc ctcgatgatc gatgcgccac
     1741 tcggtgatcg caccgcgtgg aagccgacct ggcccgccgt cgaggaagtc tacattccga
     1801 tcggcgaagc ccgcgtcgcg cgggaaggtg cgcacgtcac cgtgctgacg tacggccgca
     1861 acgttccgat gtgcctcacc gccgccgaag agctcgcgcc cgacggcatc gatgtcgaaa
     1921 tcatcgacct gcgctcgctg cacccgtacg actggccggc gatcaaagcg agcgtgcaga
     1981 agaccaaccg cgtgctgtgc gtcaacgagg acaccgaggt cacgaacttc ggcgagcatc
     2041 tcgtgcgccg gatcgtcgag gagctgttct acgagctgca cgctccgcca aagctcctcg
     2101 ccggcgcgca catcccgggc atcggcctcg cagacaatct cgagcgcgcg agcgtgccgc
     2161 acaaagacgg catcaaggct gcgctcgtcg agctggctcg ccacgagccg tgaccgtggg
     2221 agagctgagc cgctggctgc tggtcgtgcc cgcgctgcta gtcgccggct gcctgaacag
     2281 cgcgagcgtt gattgcggcg acggccgcgt ctgcccttca ggaacgatgt gtcatccact
     2341 cggatgcgtc gcgccgagcc aactcgagac gtgtcacgga atcgccgatc gcgtcgcgtg
     2401 cacgctgccc ggctcgatgc ggggcgtttg caagagcgag gtgtgcatcg cagaaggctg
     2461 tggcgacggc ttcgcgctcg gcggcgaaga gtgcgatggc gttgacttcg cattcgctga
     2521 ctgcaaggcg ctcggctacc acgacgcaac gccactcgag acatgcagag gcgactgcac
     2581 attcgatcgc tccgtctgcg gctcgcgttg cggcgacggc gtcctcgatc ccgcgttcga
     2641 agactgcgac gcatcggcgc tcgagcaagt ggccgactgc aagtcgctcg actactacaa
     2701 cgcaggcacc gtgacgtgca cgagcttctg cacgttcgac agcacgcaat gcacaggctt
     2761 ctgtggcgac ggcgtcgtca acggcggcga gttctgcgat ggcagtgcgt cagacgccgg
     2821 gtgcgcggca tttggttacg acgcaggtcg gatcggctgc gcgtcaggcg cgtgtgcacc
     2881 gctgtttggc tcgtgcgtac aactcggctg gcagccgatc gtgacggagc ccgatgtcta
     2941 cgtgaccgca ttttggggcg ctgcctccga cgacatctgg gctgtcggtg gcgatctcgc
     3001 gatcctccac ttcaatggcc aaggctggac gaaagtctcc gatgtgttcg gcggcgactc
     3061 gtacaactcg atctggggca cggcggcgaa cgatgtctgg gtcgtcggcg gcggcatcgg
     3121 cagcaagctc gcgcacctcc aaaacggcgt gttcgtcgcc gtcacgtcac cgacgacgca
     3181 gccgctgttc ggcgtgtggg gctcgtcgcc cgcaaactac tgggccgtcg gcggggcggg
     3241 aacgatcctc cactacgacg gtcagagctg gaccgtggcg ccgacactca cgaaccgcgc
     3301 attgcgcgcc gtctggggca acggtcccga caacgtgtgg gcagtcggcg acggtggcgt
     3361 cgtgctgcat cgaacttcct ctgggtggag cgtgcacacc gcgagcatga cgaacatcag
     3421 ctcgctcaac ggcgtctggg gcgacagcac gggaacggtc tggctcgctg gccaaacggg
     3481 tgacaactac tccgtgctgc tgcgcggccc gagctcttgg agtgacatgg gcgttaggtt
     3541 tttcggcgca ctcacctcgg tatggggttc cggaccgaaa gacgtgtggt tcgtcggcgg
     3601 tgacaacgag gacatcgaca ccgcgcagat ctttcactac gacggtgcgc gcatcaccga
     3661 gcacgagaca ccggcgggcc tcaactcgct ttcgtacgtg tggggcaccg gtccgaacga
     3721 cgtgtggtca tacgcgatcg gggagcagtt cacgcacaac gaaggaaccg gatggcgccg
     3781 tccgcagacc ggcgatgtgg cgcctgcatt ctttcacccg tgggacatct atggggcgag
     3841 cccgacgagc atctggatcg tcggcgacaa cttcgacgac ggaatcaccg ttcgctacaa
     3901 cggaactaac ctcgtcgcgg tcacaccaac gttcccggat ctcctctacg ccgtcacggg
     3961 ctccgggccg aacgacgtct gggccgtcgg ccagagcggt atcgtcgtgc actcgacgaa
     4021 tggcagctcg tggaccgaga tcacgcctcg cccgttctcg acggatctat tcgccgcgtg
     4081 gtcacccgga cccaacaagc tcattgcctg tggcaacggc gtggtgatcg agttcgacgg
     4141 tcaagactgg tccgggcact cgattgatgg ctcgtgctcc ggcatttggg gaagcgctcc
     4201 caacgatgtg tgggccgtcg gcagtcacat ctggcactac gacggcacga cgtggacaca
     4261 attgccgtcg agtcctgcac cggatggtct cttcgagatt tggggcagcg gaaagaacga
     4321 tgtgtgggct gccggtcgca acggggctgt cgtccactac gacggcgcga catggacgag
     4381 catcgacacg gtcgcggtcg gcttgctcac aacgctgtgg ggcacggcgc caaacgacat
     4441 ctggatggcg ggacagctcg gcgcggtcac gacggtctat cactacgacg gcattggttg
     4501 gctcgaggtc gatgtggccg ccagcagcga ctggatcagt gcgatcggcg gaacggcacg
     4561 tacgatcctg ttcggcggac gcaacgcaaa caacgccagc ttcgtgcgcg ttctcgaccg
     4621 tacgtcgcca tggagttgct cgacgatgga gagtgcgtgc aacgacgcgg tcgataatga
     4681 ttgcgacggt aagatcgacg acgaagacgt cgactgtccg taaggctcgt gtaacgccac
     4741 ggccacgccg aaaattcctg tggcacgtgt ggcgggctct tgatacgccc ttcgccatga
     4801 agcgactgtg gtggactctg gtgatcgtcg gttgtgcgag cgcatcgcgt gaaacgacga
     4861 tgcctcgcgc caatatcggc acgaagtacg cgcgtcagac aggctacttc gcgtcgtgtg
     4921 gtgcgcgctg cgacgaagaa accacgagcg gcgggatccg ccacgactcg attctcgacg
     4981 aggtcacgct cgcgaaagtc gacgcgcaag agacgtgctt cgacgtcacg atccgcgccg
     5041 aagagagcaa ggacgagccg ttctcggaga ttcatccgca gtgctcgatc gatggtggtt
     5101 cgcagcagag cgcgatcgag agcgagctcg tctcggtgtt cgactacaac tacacggggc
     5161 agcagaacgt ggctgtcgtc gaaggtgttg ctgcgagcca gtatctcggc atggcggtct
     5221 cgaagcccgc ggacatggtg ttccgcgtga tcgagcgcaa gggaatgatc tgctgcggtc
     5281 gccccgcatc gacgagcgcc gcgctcgagt tccgcaacgc gcactatgac tacggtgcga
     5341 gcaagggccg cctgcagatg cgctggaagc tgacgaactg atgcagctca cccaccggcg
     5401 ctgggtcgcg cgcgcggtcg ccgctctcgt ggcgtgctcg gtcgtgcggc tcgcagtgcc
     5461 gtggtggatt ggacgtgatg gcgagcgctg gtacgacggc gatgcggagc tgcagcgtgg
     5521 tcttgcgaac gagctcgtcg cattcgagca ctccgacgac gtcgatgctg cgagaccacc
     5581 gcgcgatcgt ttcaccggtg agtgggcgct cgtaacgcac cagatgatcg cgctcgggct
     5641 cgcgcaactc tgcctggccc accccgagtg gaagcctgcg ctcgcgcctg tcgtgaccac
     5701 cgcagcgacg aagagctttc ggcgggagat gcgcgggttc ggtacgcaag cgtggcgcgg
     5761 cgaagatgcg atggcgagcc tcgcaaccga tcacggccac gcatacctcg catatgccgc
     5821 actcgcgctc gggatggctc gtctcgtcga tccggcattt ccgcctgcgc tcgcgcgcga
     5881 gcacgatgcg ctcatcgccg cgtacgagcg acgactgctc gcctcgccga ccggcctcat
     5941 cgagacatat ccgggcgagg catatcccac cgacgtcgcc gccgtcgccg ccgccatcgc
     6001 gatccacggc cgcgcaacgc acacctcaca tgccgccgtc ctcgagcact gggcgcagcg
     6061 cgtgcgcaca gtccaaatcg atcacgcgag cggcctcgtc ctccagcgca tggacgcgac
     6121 gaccggcaag ccgcacgacg ctccacgcgg atcgggcacg gcgctcgcgg catacttcgc
     6181 tggcttcgct gaccgcgata tcgcaaagct cctcgggcaa ggcgtgctcc ggcacgagtc
     6241 cacgcgcttt ggcttcggcg gcattcgcga gtacgccgcg ggccactcgg gcgcgggcga
     6301 tgtcgacagt ggcccggtcc tcctcggcat ctcggtgtcg gcgacaggct ttgcactcgc
     6361 gcccgcccgc gcgttccacg accacgacgc gttcatctcg ctctatcgca cggtcgcact
     6421 gttcggcatg cctgctgatt caggtgaccg ccatcgcttt gcgatcggag gcccgatagg
     6481 caacgcactg ctactcgcgc tgctgacgtc tggcccggag ctcgcaccat gaagcgcgcc
     6541 tggtggcccg aagcaaaacc gttcgtcggg cccgccgcga tcgtcgtgat ctacgcgatc
     6601 gtccgcttcg cattcatcac cgtcgccgcc ggacaaggcg tgctgtcgcc atccggcaac
     6661 gtcgactcgg cggtcgccgc acttgccctc gcaacgttcg tgctgcggtt cctcgcgctc
     6721 ttcgtcgtcc cgatgacggt catctatcga ctcgtgatgc gggtctcccg acggtggaca
     6781 gcgccgactt cccgttgaaa cgccgcggct gccccctcta caatcgcgtc gcatgacgct
     6841 gtcggtggac ccaccctcga tgaaacaccg cgacatcgac ggcgttggcc tcggcctcgt
     6901 cgcaacgatg gacctactgc cggtgggcgt cgctcacttc gcgcccgatg gcacgttcct
     6961 cttcgtcaac gaggcgctct ctgaaatcct cggcttcact cgccaagagt tgctgacaaa
     7021 atcgtttcag cagatctcgt tccccgacga tctgcacgaa tgcctgcgct tgaacgcgga
     7081 gctcgcgacc ggcgctattc ctcgctacgt ccacgagaag cggttcatcc gcggtgacgg
     7141 cagcatcgta tgggtacgcg ttacggtcac cgcggcacgc aatcgcccgg cgccggccgt
     7201 cgcgttcttc gtcgcagtcg ccgaagacat caccgcgcaa cacctcgcgg aggaagcgcg
     7261 ccgcaccgcc gaagctcgcc tccagctcgc gctgcgtgcc gccgacatcg ggatcttccg
     7321 ttgggacctc gtggccgacc gcctcgaagc agacgaaaat ctgctccagc tcttcgccct
     7381 cgcacgcgac gagtcgacga agacgcaagc gttcctctct cgcatccatc ccgacgaccg
     7441 tccacgcctc gaagaagcaa ccgcacgctc gatgcacggc gccgagatcg acgaagaatt
     7501 ccgaatcatc ctgcccgacg gcacggaacg ctggatccgc gatcgcgcgc gcacgctgct
     7561 cgcggacgac ggctccccga tctgcgtcac cggcgcctgc gcagacgtca ccgcacgccg
     7621 tcagctcgac cagacggtgc gccatgcgct gcaaaccgcc gaacgcgccg tcagtgctcg
     7681 cgacgaagcc atcgcgatcc tcgcgcacga tctgcgcaac ccgctcggta atatctcgct
     7741 cgcatcggca gaggtcccga gcgatgaagc cgcgcgcgta cgtcggttcg cgatcatccg
     7801 gcgcgccgcg atcgcgatgg agcgcttgat cagcgacctg ctggatatcg cgcggatcga
     7861 tgcaggcacg ttcgcagtag aaaaggcacc gaccgacatc cgctcgctcc tcgacgaaac
     7921 cgtcgagctg tttgcggagc ccgccgcgaa cgcaggcgtc cagctgcgat gcacggtcga
     7981 cgacggcgtt ccatcgtcgg cgctcgccga ccgcgagcgt ctccgtcagg tcttgtcgaa
     8041 cctcgtcggc aacgcgctca agttcacgcc acgcggtcgc cacgttcata tcggcgcatc
     8101 cgcagacaac gcgggcggcg tcctcttcac tgtccgcgac gaaggttgcg gcatcccgag
     8161 cgagcagctc gcgcacgtat tcgaccggtt ctggcaagcg aatcgcggca agcgttcggg
     8221 cgcggggctc ggtctcgcaa tcgtcaaagg catcatcgac gcacacggcg gctcgctcgc
     8281 gctcgagagt gaagtcggcg ccggcacgaa ggtcatgttc tcggttgagt cccgcgggtg
     8341 acttgtcgtc tagcgccggc gacggccgaa tgagccgccg gcaacgcgaa atcagtagcc
     8401 actgcggcgc gctcctaacc acgagaaatg gttgccgtcc gcgatggcac gtccgctgct
     8461 ttagcgacgg accatgcaag acatcaaact cgaagacctc gtgaacgtta ccggtggtgc
     8521 aggcgtgctc gacaacatgg gcaccggcgc ttcgaatctc agcaaccgct gggcgaacaa
     8581 cacggcgaac acgctcggcc gtaattcgtt cttcggtggc gtcggcgccg ctgccgcggg
     8641 cttcgcgggt ggcatcgttg gtggcctgca aggcttcggt cacacgctga ccgagaaggt
     8701 tccgctgccg ccgaagtgat catgcgacga tcaggaatcg ctcggcgcgg cgtgccctgt
     8761 gcaggatcag cgccgcatgc tgcgcaaccc attgattctc gctatcgcca ctgcggcgct
     8821 cgcgctcgta gcgtgcgctg ataaaccagc gacgctcgac atcgagagga aagcacagcc
     8881 tccgtcgatc aactcctgcg acgacggggt cgaggcttgc gctggcaatg cccagtgcga
     8941 gtcatgcctc atcgaaggtg agtgcgcggc gggtccgagt cccgtttgga ccggcacggc
     9001 gtgcgtgtgc acggacaaca accctgcgaa ctgcgtctac gaatactgct gcgcgctcgg
     9061 ctacgaatgg aactccagcg catgcagatg tctcccgcac gggcggtagg gtccggcacg
     9121 cgagcacagc atactgcacg accgtggatc acgacgtgta gaggtgcgac gctcccggca
     9181 tggaacgcgt cgcgcatcgc atctgccctc tgtgcgaagc cacgtgcggg ctcgagctcg
     9241 cggtggaggg cgacaaggtc acgtcgatcc gcggtcacgc ggctgacgtt tttagccaag
     9301 gcttcatctg tccgaagggc gccgcgctcg ccgagctgca cgaggatccc gatcgcttgc
     9361 gagcaccgct cgtcaaacgc gacggagtcc acgtcgaggt gtcgtgggac gaagcctttg
     9421 ctgagatcga acgccggttg ccgccgattc tcgccgcgca tggtcgggac gccgccgcgt
     9481 tgtcgctcgg aaatccgagt gcacaccgct acggcctgct gctctacggc gcgcgactcg
     9541 cgcgtgcgat tgcgacgacg aacgtgttct cggcgtcgac gctcgatcag atgccgaagc
     9601 agctcgcgtg cgggatgatg ttcggccact ggctcagcgt gccggtgccc gacctcgacc
     9661 gctcgcagct gctcgtcgtg ctcggcgcga atccagtcgt gtcgaacggc agcatgtgga
     9721 ccgttcccga cttccgcggc aaggccaagg cgatgcaagc ccgcggcggt cgcctcgtcg
     9781 tgatcgatcc gcggcgcacg gagacagccg agctcgcaga ccagcacatc ttcatccgac
     9841 ccggcggcga cgtcttcctg ctcctgggca tcgtccacgt gctgttcgcc gaaaagctcg
     9901 tccgcctagg ccggctcgca gatcacgtcg acggtgtcga ccagatcgaa cgcgcggtcg
     9961 ccgacctctc gcccgaagcc gtggcctcgc gctgcggcat cgagccatcc gtgattcgcg
    10021 atctcgcacg tgcgctcgcg acgacagaga gtgccgccct ctacggccgc atcggcacgt
    10081 gcacgcagga gtacggcacg attgcgagct ggctcgtcga cgtgatcaac gtgctcaccg
    10141 gccatctcga cgcaccgggt ggcgcgatgt ttccgaaggc cgcggcattc gccgcgaaca
    10201 cgatcggctc gccgggcaaa ggcaaaggca tcgccatcgg ccgccgcaag agccgcgtct
    10261 cgggtgcgcc ggaagtgttc ggcgagctgc cgatgatctg tctcgccgaa gagatcgaga
    10321 cgccgggcga aggccaagtt cgcgcgctca tcacgatcgc gagcaacccc gctctctccg
    10381 cgccgaacgg cccgcgcctc gcagccgcct tcagctcgct cgagctgatg gtcagcgtcg
    10441 acatctatct caacgagacg acgcgccacg ccgacgtgat cctgccgggc ctctccgccc
    10501 tcgaggaatc gcactacgac gtcgcatttc cgcagctgtc ttgtcgcaac gcggcacgct
    10561 acagccccgc gatcttcgct ccacccgcgg gcgcgctcgc cgagtggcag atactgctcc
    10621 gcctcctcgc gatcgccaca ggtcgcggcg ccaacgccga tatcaacggg ctcgacgacg
    10681 agctcacgct cgcagacgtc cagcgcgtcg caggcgcgca agctccgctc ctcctccagc
    10741 agctcggcga tctcaaaggc ccagagcgcc tcctcgacct cgcgctccgc ggcggcccgt
    10801 acggcgatcg cttcggcttc gttcccgacg gactcaacct cgctcgcatc aaagcggcac
    10861 cagctggcat cgacctcggc gcgcttcagc cacgtatccc cgaaatgctg cgcacgccgt
    10921 cgggcaagat cgagctcgcg ccaccgctgt tcatcgatga tctcgggcgc gtccacgccg
    10981 acctcgcgcg tcccgccccc gacctcgtca tcgtcggccg ccgccagctc cgctcgaaca
    11041 acagctggat gcacaacctg ccgatgctgg cgaaaggccc attccgctgc gtcgcgctcg
    11101 tccatcccgc cgatgcagaa cgtcttgccc tcacgaacgg cggacgcgca cgcatcgcca
    11161 acgcgagccg cacgatcgac gtcacggtcg aagtaagcga cgagatgatg cccggcgtcg
    11221 tcagcttgcc gcacggctgg ggccacgatc agccaggcac aaagctcacc gtcgcggccg
    11281 agcgcgcggg cgcaaacctc aacgcgatca tcgacgacac gcgccgcgat ccgctctccg
    11341 gcaacgccgt gctctccggc attccgatca cgatgtcgcc gctctaagcc gcccgacgcg
    11401 agcattgtct cactccgaaa attctcgact cgacgacgtc tgcctacgtg cgcacatgtg
    11461 tgccggccgc gactgcactg ttggccatcg cacgaacgtt acgagtcgcg cgcaagcact
    11521 cacgcttcca tcacgaaggt cgctcgtcga aaacgacgcg attcacgtaa cgtcttcacc
    11581 accgatgcac gatgactcga tccagggcgc actcgaatcg tacgttcaga cgcagtccga
    11641 cttcgccgat tcggcagacg acaatccgtc ggtcggcacg ttcgtcgagt ggccgcccaa
    11701 accgccaccg cctgccgcgc tcgcgagcat cgtgcacagc aacgctcgga tcgagctgcc
    11761 gccagccgtc gacgagatcg agtcgattgt cgtgctcgac gccgacgacg ttgacgacgt
    11821 cccgtaggcg acgcgagcag tcgtcgcagg tgtaatgcga gcgggctagc tgcgtcgtgc
    11881 gcgtagacct cacaaggagc tacgcatgtc gccgaatccc gatccccgcc gatggtgggc
    11941 cctgttcctg ttatgcaccg cgcagtttgt agtcatcctc gacacctcga tcgtcggcat
    12001 cgccttgccc gcgttacagc gcgcgctcga cttcgacgca gcgggcctgc agtggatctt
    12061 caacgcgtac gtcgtcgcct tcggcggctt acttcttctc ggcgggcgcc tcgctgacat
    12121 cttcggaccg cgtcgtctgt tcgcaacggg cttcggcatt ctgaccggcg catcactcct
    12181 cgctggactc gcgacgaccg gcaccacgct ccttatcgcg cgtgcgctgc aaggcgcagg
    12241 tgcagcgttg atcgcgcctg ctgcgctctc gatggtgatg gggctcttcg cggcgcgccc
    12301 gaccgagctc cgaaaggcgc ttggcttctg gggcgcgtcc gctgccgctg gtggtacggc
    12361 aggcgtcttc ctcggtggcg tcatcaccga gtggctcagc tggcggtgga cgttcctcgt
    12421 gaacgtgccc gtcggcgtgc tcgtgctcct cgcgacgaac gcttggctgc cgcgtggttc
    12481 acgtcagcaa ggaagtgtcg acgtcctcgg cgcatcgacg atcaccggcg gcctcgcgct
    12541 caccgtctac acgatcgtga ccgcgaatgc ggcaagcagc gcgcgtacgg tgattctcct
    12601 cggactcagc gcagcgctgt tccttgcgtt cgttctcatc caacgcgtgc ggacgtcgcc
    12661 gcttgttccg ttctcgatct tc
//
LOCUS       DATLBZ010000469         6066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1335036, whole genome shotgun sequence.
ACCESSION   DATLBZ010000469 DATLBZ010000000
VERSION     DATLBZ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335036"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /gene="mutL"
                     /locus_tag="VMZ53_23815"
     CDS             complement(<1..1910)
                     /gene="mutL"
                     /locus_tag="VMZ53_23815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HUS31562.1"
                     /translation="MESQIAVLPATVIDQIAAGEVIERPASVVRELVDNAIDAGAKTI
                     SVETTAGGRGLIRIVDDGCGMSPKDAVLAFERHATSKLRAVDDLWGLPTLGFRGEALP
                     SIASVSRITLTTRRQQDLAATRVQIEGGKLISVSEVGAPVGTCVEVTDLFYCVPARLK
                     FLKGEGTEASHITDHVARIAMAHFALHLRLRHNGRVALDVPPDRDAFSRAQSLLGSRV
                     ASRMIPVSGEETGIRVTAFLGAPDLAQTTARGVQLFVGKRPVRDRGLLHALAMGYGEL
                     LPRGRYPLAIVLLEVPGGSLDINVHPQKAEVRFSDAAMVCAAVRHVVQAGVERAAWRN
                     DQSGVMMTAIASIAPPRLPFDAPATSLAHTYAAQLRESREGGWAARDASSTQAQLGFD
                     ASPRMWAQSIKDRVRTSRAAEAELAARAAHAIAKVADADRPSRPQRLDDGAPLDDAQL
                     AEGSAPTPTPGFFSTLRYLGQLDLTYLICEGDGELVLIDQHIAHERVELARLKALQSE
                     RDVVPTQRMLFPQTIDVKPELVELATKMAPVLAQVGYEAEGFGKHTLAVKAVPGGIRH
                     GDPTLLLRELLTEWADEGTPTEAERLERVLAEIACHSVVRAGDRLSPSEAEALLRSMD
                     GADFTTHGPHGRP"
     gene            1977..2981
                     /locus_tag="VMZ53_23820"
     CDS             1977..2981
                     /locus_tag="VMZ53_23820"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUS31563.1"
                     /translation="MPSTQTQGRVVAVTGACTYLGVELLRRLEEDPRYGKVLALDVRE
                     PPVRGGKIEHVMLDLTQPTVDGELATLLERHRVDTFVHGAFLSHPTHAADWAHELEDV
                     GTMHVLNACAGVEPRRLVMISTTLVYGAHPKNPNFLTEEAELRGHRDSRFVNDKVRAE
                     RQVQRFAKEHPGVEVCVLRFAPILGPTVSNMYTRFFSRVVAPVMMGHDPLMQFVHEQD
                     AAFALKAAVDSKVRGAFNIVGKGVLPYTTVLALLGRVPVPMPQIVARQLSKVLWATQL
                     VGSPPSFLDFLLYLCVADGTKAKRELGFSARLSIKRTILDFLGVAPEDGATDVARAQA
                     "
     gene            2978..4081
                     /locus_tag="VMZ53_23825"
     CDS             2978..4081
                     /locus_tag="VMZ53_23825"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUS31564.1"
                     /translation="MTDETDEDPVIRDESDDEDWYGDADPRVPDAATAWPTNDNGDLD
                     FLGHRDGGYIPSDPHEGEAELRELERRLEQNRSPVFPIDVRKHLPLEAVWRRWRALAM
                     WGRSDVVDDFGRDPRATARWEWLFEFLYSKWFRVHASGLENIPERGRALLVANHAGTL
                     PYDSAMVMHAVRRDHPARRDVRPLVEDTVFHLPFLGPVMNRIGGVRADPANAERLLEK
                     DELVAVFPEGEKGMGKLWKDRYRLQRFGRGGFVKLALRTGAPIIPVAVVGAEEAAPML
                     GKVTWFAKNIGIPWIPVTPTFPWIGPAGLLPLPSKWMIEFGEPIELPHGKEAAEDRLL
                     VNKLADQIRTQIQTMVDGLLSKRRSPLFAAFFG"
     gene            4159..4698
                     /locus_tag="VMZ53_23830"
     CDS             4159..4698
                     /locus_tag="VMZ53_23830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31565.1"
                     /translation="MAAAFESELDRLLDQVRELLGAGDDALLAEANVAVNHALQLSPD
                     SVEAWLLKAQVASATGDDYAALAAVEMALKRSPHRAEALYWRGAVLGDLDRHREALRS
                     IEAAFRVLADADHWLLEDLFYEKVMILDALGLHDAAVAACEAGLDRCPGSPLLRAALA
                     PAERARVRGSLKVLRGGAG"
     gene            4744..5919
                     /locus_tag="VMZ53_23835"
     CDS             4744..5919
                     /locus_tag="VMZ53_23835"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS31566.1"
                     /translation="MMKISTSLVVALGLVSVAGCKSKHEEGTVQKPTEGVQKDTGSAG
                     ASMKAPEEKPLTGADLAAKYQKCIGQINDGKFEDFKKDCMSGEYAVHQYGQDMKGSDM
                     VIGWFNAMKKAMPDWKMMPQIVLVDGRNILAVNLTTGTHSGELAMPGMPPIPATNKKI
                     GMFFVHKLAINEQNKATEEWGFDDPGTMMGQLGLSPKDAPPSRPAVDKGIDGAPILVV
                     TADDAKEKANLETFKKGMDAVNSHKLPDIMATMTDDAIEADMAEGADRKGKKDIEAGM
                     KGFLTAFPDVKVTASEMHAAGDWVIAIGKMEGTNTGDMGKMKKTGKKVSLDYAEVAML
                     KDGKIAQLWRFRSGMQAAMQMGLMPAPGAAPAAGSAAPAAGSAAAPAAGSAAAPATK"
BASE COUNT         1012 a         1931 c         2129 g          994 t
ORIGIN      
        1 gggcggccgt gtggaccgtg cgtcgtgaag tcggcgccgt ccatcgagcg cagcagagcc
       61 tcggcttccg agggcgatag acgatcaccg gcgcgcacga cggagtggca cgcgatttcg
      121 gcgagcaccc gctcgagacg ctcggcttcg gtcggtgtgc cttcgtcggc ccactcggtg
      181 agcagctcgc ggagcaggag tgttgggtcg ccgtggcgga tgccgccggg cacggctttg
      241 acggcgagcg tgtgcttgcc gaagccttcg gcttcgtagc cgacttgcgc gaggaccggc
      301 gccattttcg tcgcgagctc gacgagctcg ggtttgacgt cgatggtttg ggggaacagc
      361 atgcgctgcg tcggcacgac gtcgcgttcg ctttgcagcg ccttgaggcg cgcgagctcg
      421 acgcgttcgt gcgcgatgtg ttgatcgatc aggacgagct cgccgtctcc ttcgcagatg
      481 agataggtga ggtcgagctg gccgaggtag cgcagcgtgc tgaagaagcc gggcgtcggc
      541 gtgggtgcgc tgccttcggc gagctgggcg tcatcgagtg gtgcgccgtc gtcgaggcgt
      601 tgtgggcgtg acgggcggtc ggcatcggcg actttggcga tcgcgtgagc ggcgcgcgcg
      661 gcgagctcgg cttctgcggc acgcgatgtc cgcacgcgat ctttgatcga ctgcgcccac
      721 atccgcgggc tcgcgtcgaa gccgagctga gcttgcgtgc tcgacgcgtc gcgcgcggcc
      781 cagccacctt cacgggattc gcgaagttgt gcggcgtatg tgtgggcgag cgacgtggct
      841 ggcgcgtcga acgggaggcg cggcggagcg atggacgcga tggcggtcat catcacgccg
      901 gactggtcgt tgcgccatgc ggcgcgctcg acgccagctt gcacgacgtg gcgaacggcg
      961 gcgcacacca ttgcagcgtc cgagaagcgg acttccgctt tttgcgggtg gacgttgata
     1021 tcgagcgagc cgccgggaac ttcgagcagc acgatcgcga gtgggtagcg gccgcgtggg
     1081 agaagctcgc cgtagcccat tgcgagcgcg tggagcaggc cacgatcgcg aacggggcgc
     1141 ttgccgacga acagctgcac gccacgcgcg gtggtctgcg cgagatcggg cgcaccgaga
     1201 aatgcggtca cgcgaatgcc ggtttcttcg cccgagaccg ggatcatgcg ggaggcgacg
     1261 cgcgagccga ggagcgattg ggcgcgagag aatgcgtcgc gatcgggcgg gacgtcgagc
     1321 gcgacgcggc cgttgtggcg caggcgcagg tgcagcgcga agtgcgccat cgcgatgcgc
     1381 gcgacgtggt ccgtgatgtg cgaggcctcg gtgccttcgc ctttgaggaa cttgaggcga
     1441 gcgggcacgc aatagaagag gtcggtgacc tcgacgcacg tgccgaccgg cgcgccgacc
     1501 tcggagaccg agatcagctt gccgccctcg atctggacgc gcgtcgccgc gaggtcctgc
     1561 tggcggcggg tcgtgagcgt gatccgcgag accgaggcga tcgatggcag ggcttcgccg
     1621 cggaagccga gcgtgggcag gccccacagg tcgtcgaccg cgcggagctt cgaggtggcg
     1681 tggcgctcga acgcgaggac ggcgtccttc ggggacatcc cgcagccgtc gtccacgatg
     1741 cggatgaggc ctcgcccgcc cgcggtggtc tctaccgaga tcgtcttggc acccgcgtcg
     1801 atggcgttgt cgaccagctc gcgaaccacc gacgccggcc tctcgatgac ctcacccgcg
     1861 gcaatctgat cgatgaccgt tgccggcaga acggcgatct gggactccac ccactggggg
     1921 tatcacaccc ccctgaccac tcatgatctg ggccggtttt gcgctataag ccccccatgc
     1981 cgagcacgca aacgcagggc cgcgtcgtcg cggtgacggg tgcgtgcacg tacctgggcg
     2041 tcgagctgct gcgccggctc gaagaggacc cgcgctacgg caaagtcctc gcactcgacg
     2101 tccgtgagcc gcccgtgcgc ggcggcaaga tcgagcacgt gatgctcgac ctcacgcagc
     2161 caacggtcga cggcgagctc gcgacactcc tcgagcggca tcgcgtcgat acgttcgtgc
     2221 acggcgcatt tctctcgcat cccacgcacg cggcggattg ggcgcacgag ctcgaagatg
     2281 tcggcacgat gcacgtgctc aatgcgtgcg ctggcgtcga gccacgtcgc ctcgtgatga
     2341 tctcgacgac gctcgtgtac ggcgcgcatc cgaagaatcc gaacttcttg accgaggaag
     2401 ccgagctgcg cggccatcgc gactcgcgct tcgtcaacga caaggttcgc gccgagcgcc
     2461 aggtgcagcg cttcgcgaaa gagcatccag gcgtcgaagt ctgcgtgctg cgatttgcgc
     2521 cgatcctcgg cccgaccgtc tcgaacatgt acacgcgctt cttctcgcgc gtcgtcgcgc
     2581 ccgtgatgat gggccacgat ccgctgatgc agttcgtgca cgagcaggat gcggcgttcg
     2641 cactcaaggc ggccgtcgat agtaaggtgc gcggcgcgtt caacatcgtc ggcaagggcg
     2701 tcctgccgta cacgacggtg ctcgcgctgc tcggtcgcgt gccggtcccg atgccgcaga
     2761 tcgtcgcgcg ccagctcagc aaggtgctgt gggcgacgca gctcgtgggc tcgccgccga
     2821 gcttcctcga tttccttctc tatctctgcg tcgccgacgg cacgaaggcc aagcgcgagc
     2881 tcggcttctc ggcgcggcta tcgatcaagc gcacgattct cgatttcctc ggcgtcgcgc
     2941 cggaggatgg agcgacggac gtcgcgaggg cacaggcgtg accgacgaga ccgacgaaga
     3001 tccggtgatc cgcgacgaat ccgacgacga ggattggtac ggcgatgccg atccgcgcgt
     3061 gcccgatgcc gcgaccgcgt ggccgacgaa cgacaacggc gacctcgact tcctcggcca
     3121 ccgcgacggc ggctatatcc cgagcgatcc gcacgagggc gaggccgagc ttcgcgagct
     3181 cgagcgccgt ctcgaacaga accgctcgcc ggtgttcccg atcgacgttc gcaagcacct
     3241 gccactcgag gcggtgtggc gtcgctggcg tgcgctcgcg atgtggggac gcagcgacgt
     3301 cgtcgatgat ttcggccgcg atccacgcgc gaccgcgcgc tgggagtggc tcttcgagtt
     3361 cctgtactcg aagtggttcc gcgtgcacgc gagcggcctc gagaacattc ccgagcgcgg
     3421 ccgcgcgctg ctcgtcgcga atcacgcagg cacgcttccg tacgacagcg cgatggtgat
     3481 gcacgccgtg cgtcgcgatc atccggcgcg tcgcgacgtc cggccgctcg tcgaggacac
     3541 cgtcttccac ctgcccttcc tcgggccggt catgaatcgc atcggcggcg tgcgggccga
     3601 tccggcgaat gccgaacgcc tgctcgagaa ggacgagctc gtcgccgtct tccccgaagg
     3661 cgaaaagggc atgggcaagc tgtggaagga tcgctaccgc ctgcagcggt tcggccgcgg
     3721 tggcttcgtg aagctcgcgc tgcgcacggg cgcgcccatc attccggtcg ccgtcgtcgg
     3781 cgccgaagaa gccgcaccga tgctcggcaa ggtcacgtgg ttcgcgaaga acatcggcat
     3841 cccgtggatc ccggtcacgc cgacctttcc gtggatcggg ccggccggcc tgctgcccct
     3901 gccgagcaag tggatgatcg agttcggcga gcccattgaa ttgccgcacg gcaaagaggc
     3961 cgccgaagat cggctgctgg tgaacaagct cgccgatcag atccggaccc agatccagac
     4021 catggtcgat ggtttgctct caaagcgccg ttctcccttg tttgccgcgt tcttcggcta
     4081 gtagggaaga aatcacggag ggcctccgta gcccgtagct ttccttgaac ggttggatct
     4141 gcgtgttatc gtgaccgcat ggctgcggcg ttcgagtccg agctagaccg cctgctcgat
     4201 caggtgcgtg agctcctcgg cgccggtgac gacgcactct tggcagaagc caacgtcgcg
     4261 gtgaaccacg cgctgcagct ctcgcccgac agtgtcgaag cgtggctgct caaggcgcag
     4321 gtcgcatcgg ccaccggtga tgactacgcc gcgctcgccg cagtcgagat ggcgctcaag
     4381 cgatcgccgc atcgtgccga agcgctgtac tggcgcgggg ccgtgctcgg cgatctcgat
     4441 cgtcatcgcg aggcgctgcg ctcgatcgaa gcggcgttcc gggtgctcgc cgatgcggac
     4501 cactggctgc tcgaagatct cttctacgag aaggtcatga tcctcgacgc gctgggcctg
     4561 cacgatgccg cggtcgccgc gtgcgaagcg ggcctcgatc gctgccccgg atcgccgctg
     4621 ctgcgtgcgg cactcgcgcc ggcagaacgc gctcgcgtac gcggctcgct caaggtactg
     4681 cgcggcggcg cgggttgatt cctgatccac tgggtgcgaa gttcgcgccc agaggagaac
     4741 aggatgatga agatttcgac gtcgcttgtg gtcgcgctgg gtttggtttc ggtggcgggc
     4801 tgcaagagca agcacgaaga gggaacggtc cagaagccca ccgagggcgt ccagaaggac
     4861 accggctctg caggcgcgtc gatgaaggcg cccgaggaga agccgctcac cggcgctgat
     4921 ctcgctgcga agtatcagaa gtgcatcggc cagatcaacg acggcaagtt cgaggacttc
     4981 aagaaggact gcatgtccgg cgagtacgcc gtgcaccaat acggtcagga catgaagggc
     5041 agcgacatgg tcatcggttg gttcaacgcc atgaagaagg cgatgccgga ctggaagatg
     5101 atgccgcaga tcgtgttggt cgacggtcgc aacatcctcg ccgtgaacct gacgaccggt
     5161 acacactcgg gcgagctcgc gatgccgggc atgccgccca ttccggcgac gaacaaaaag
     5221 atcggaatgt tcttcgtcca caagctcgcg atcaacgagc agaacaaggc caccgaagag
     5281 tggggcttcg acgatccggg cacgatgatg ggtcagctcg gcctctcgcc gaaagatgcg
     5341 ccgccctcgc gtccggccgt cgataagggc atcgatggcg cgccgatcct cgtcgtgacc
     5401 gccgatgatg cgaaggagaa ggcgaacctc gagacgttca agaagggcat ggacgcggtc
     5461 aattcgcaca agctgcccga catcatggcg accatgaccg acgatgcgat cgaagcggat
     5521 atggccgaag gcgccgatcg caagggcaag aaggacatcg aagccggcat gaagggcttc
     5581 ctcaccgcgt tcccggacgt gaaggtcact gcgagcgaga tgcacgcggc cggcgattgg
     5641 gtgatcgcga tcggcaagat ggaaggcacg aacaccggcg acatgggcaa gatgaagaag
     5701 accggcaaga aggtctcgct cgactacgcc gaggtcgcga tgctcaagga cggcaagatt
     5761 gcgcagctgt ggcggttccg cagcggcatg caggcagcga tgcagatggg cctcatgccc
     5821 gcgcccggtg ccgctcctgc cgcaggcagc gcagcccccg ccgctggtag cgccgctgca
     5881 cccgcggccg gcagcgcagc cgcgcccgca acgaaatagc gcgaacggcc gtaccgcgcg
     5941 cgcggcgaag cgccttgcta tcatctgcgc gtgcgtgttc gccgcatcgc tgggctcgct
     6001 gcgagcggca ttgtcgccgt cgcttgcgtg tggctcgtcg gcggcgcgat cgcgcaggtc
     6061 tcgctg
//
LOCUS       DATLBZ010000470        18772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5062735, whole genome shotgun sequence.
ACCESSION   DATLBZ010000470 DATLBZ010000000
VERSION     DATLBZ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18772
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5062735"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VMZ53_23840"
     CDS             complement(<1..794)
                     /locus_tag="VMZ53_23840"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HUS31567.1"
                     /translation="MLQSQSQSQGGGLFGSYTALPGTYDEMFDSGSPRAPFARVAQLL
                     AKMGTDELARAQTLAEHSLLRQGVTFSVYGDARGTEKIFPFCLLPRLIAGADWTRLER
                     GLQQRLRALGMFLDDIYGEQKILAAGIVPAEMILGAAAYRANLRGLKPPGGVRIHISG
                     IDLIRDPAGTWRVLEDNLRTPSGVSYVMENRQLSKRMFPRVFDAARVHRVDHYPTRLA
                     ETLRSVSPAPDGETYAVVLTPGPYNSAYFEHSFLARTMGLELVQAP"
     gene            826..1302
                     /locus_tag="VMZ53_23845"
     CDS             826..1302
                     /locus_tag="VMZ53_23845"
                     /inference="COORDINATES: protein motif:HMM:NF027223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase
                     domain-containing protein"
                     /protein_id="HUS31568.1"
                     /translation="MIVHKPPTPWTARPQIPTVFLAGSIEMGSAPDWQSELVRELGVD
                     ATFLDPRRAEWDSSWRQSIDEPQFRGQVEWELDGLERADVIAMWFVAETKSPITLLEL
                     GLHARSGKLVVGCPHGFWRRGNIEVVCARYGVPLSAEWDAFVGSVSQTIMDTRRRE"
     gene            complement(1277..3895)
                     /locus_tag="VMZ53_23850"
     CDS             complement(1277..3895)
                     /locus_tag="VMZ53_23850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31569.1"
                     /translation="MRAWAIVTWAIVMLVGGCISSSSVVCDDGRVCPSGTVCREALCV
                     PKTCGDGKVDPGEQCDGLDLGSLDCVDFGYYGPEGLTCSSTCFPETSTCSGGICGDGE
                     VDPQEACDLAMPADHCGDFGFDVGRITCATTCTNDVSGCDRIGWTQMPSGTSADLTAV
                     WGAGANDMFAVGTGGTILHYDGAGWSPMVSGTTADLMGISGSSSSFALAVGANGTILQ
                     WNGVAWTPMTTGTTERLNAVMVVSPARAFAVGTNDTVLFFDGGSWTKLVNAAAPGDEE
                     LLAVWANATDVLVAGFTSNHLGAMVRHYKPDQSWTIAFSEAAGGPIRALSGKAANDVW
                     MAGRNDGSVIIYQNVDQLSCSTVTCTPVYTTGNLFGIWEHTPSNVYAVGSDGAIFHKT
                     TGFSSWPARADTSHAVEDLRAVFGTDANNVFAVGANGRIVRSTAIQIESYLPKPGANY
                     AGGFGLTEDELYTVGAVGTQGVIYEYVGANNNNVWVQDYLGGEPLNAVWSTAGGPTVV
                     VGQRGVIRTSVAAPAWSAVHSDNTMTLNGIWGTSYNDLFTVGAGTNGGVILHFNGSTW
                     SAQTVPPGVGGLFSVWGTSTANVFAVGDAGVILHYDGSAWSIVRAAASSAPPLYAVWT
                     GGDEVIAVGGDIVQRHGTAWIATRAPTTLHALAGSPGNVYASGDLGMLLRWDGATWIR
                     IESGTSRQLASLSVSGHSVAFTGAFGVIQRLDLGSTATQELVCGDYWDNDLDGLQNCA
                     DPDCAATAVCSTGGLCRGATNVTCGSTPGTTRGGPARFDLFSCADSFTTGSERFFRFT
                     PAESGPVTAHLAAPSPELSIAVLRASPEGACKIDDCIGVGPDVTFEGTAGETYFFVVD
                     GPDGTAGEFTLDVACP"
     gene            complement(3922..4275)
                     /locus_tag="VMZ53_23855"
     CDS             complement(3922..4275)
                     /locus_tag="VMZ53_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31570.1"
                     /translation="MKRLIFVFGLLGLIGCFLPVAGHLSLFDLRHFSEGWHVWLVIGA
                     FALPTYVGLSKTEGDTAAAVVGTFSFGYLAYKFFGHIFDLAFHASIGGFFMAVATVLG
                     LVWSLIALVMSSSDG"
     gene            complement(4286..4456)
                     /locus_tag="VMZ53_23860"
     CDS             complement(4286..4456)
                     /locus_tag="VMZ53_23860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31571.1"
                     /translation="MAIIIGLVIGALVGWLIGKNKGLGGVGLVLGGLLGVIGWIIVAV
                     MKGTPQDPSQLR"
     gene            complement(4530..6230)
                     /locus_tag="VMZ53_23865"
     CDS             complement(4530..6230)
                     /locus_tag="VMZ53_23865"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HUS31572.1"
                     /translation="MADDLAASLEMHDELIARRGVDVWVGAEPTFTRAESVEPAWTGA
                     AEGGDKLARAHAVATAIANQLSGASVTRVVGRTFPGEEKPRFAFGVRWRDEIIGIVDQ
                     GERAAVDAEPQPPPTDLGSDHWLTVTPDPGVVEVNMAPAANTRTFYAHAQQVWRAAET
                     AGLSPTRFRFNGDVADSGGGGQITLGGSRPDTSPFIRYPHVLPALVRYVNNHPSLSYW
                     FASECVGSASQGPRPDEGARERWDELAVTLGWLEHLADRGELGPDQMQAGLSPLLVDS
                     AGNSHRAELNVEKLWSPHVPAHGPRHGKMGVVELRAIRMPDRPGMLAALAALFRSIVA
                     RLVVSHYRAPLVDWHDELHDRFALPAALHRDLRMVLGDLDEHGLGLPPQLRRELEAFR
                     SPGITCRLGDATLTLRPALEFWPLVGDVASQERATARWVDASTQRWEVSLDGAGPDRV
                     IIGGKWVHLRLVGDHVRAIGVRRRIYQPSPGLHPGLPVIDPLAIEWAWAGRSQRVELW
                     AWRPGGGPYSGLPRDTNEALERRQERIKIVTHEGDASAPTFWREARPFTIDLRRDGIA
                     "
     gene            complement(6233..9541)
                     /locus_tag="VMZ53_23870"
     CDS             complement(6233..9541)
                     /locus_tag="VMZ53_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HUS31573.1"
                     /translation="MVAWYTTFPVRVLVQHRSRYHYPRPALLGPHLVRLRPSDHARAR
                     IESYKLAVSPEHRLHWQRDPHGNRVARLTFKSGQEIRELDVVVEIAVDVGPVNPFDFF
                     VDDRAKQLPFRYPDALETELGVFLDRGDAAYRMGPLANELLAKVPASGDTVGMLVEAN
                     RLVRERVNYVIRDETGVWTPEETLANGRGSCRDSAVLLVALLRARGIAARFVSGYLIQ
                     LTDEGMIPNEPKGVSRDVVDLHAWAEAYVPGAGWIGLDGTSGLLTGEGHIPLAATATP
                     AHAAPLDGTSNVAAASVEFSTTIARLGHEARPTAPFTDEIWGELLSASDRADELLQQA
                     GVELTVGGEPTFTARDGVEAVEWQGGALGADKWQKGRALADELRDRLAPTGVVLHRMG
                     KHYPGESLPRWALDIIARRDGVALWPDRELRATGSIADAKHYGEALAEILGMRELLHP
                     AYEDPWEVMRSEAVLPPDVDPTKAGLDDPEERQRLAKIFAQGVGSAVGWVLPLARGAA
                     GWLTEAWKFRRGHLFLLPGDSPVGLRLPLGSLPEAVAPPLWADDPNVADPRRDDTDDD
                     TQDALRRESPARRVQRSESGIGIRTALAVEPRNGELWIFLPPMTRFSDFCELIAALDE
                     ARVATGFDVNLEGYGPPPSPNLRRFAVTPDPGVLEVNLPPSWSGREAAELHATVFDAA
                     LSVGLTAERYMLDGRMAGSGGGNHITLGASQPMASPFLTKPELLASLISFVQHHPSLS
                     YMFTGLFVGPTSQAPRVDEARHDALYELEIALPQLFTPGTPVWQVDALLRHLLVDVAG
                     STHRAELSIDKLFDPHTPFGRQGLVELRAFEMPPHPRMTAAQVVLTRALVAAFTQEPY
                     KHALVRWGAELHDRFLLPYYMWRDFEDVLGYLAARGVALPSEAYKPFVELRCPLVGSL
                     DVGPGRVEVRNAIEPWHVLGEEATQSGTARFVDSSMERIEIRAHGLDEERYLIAVNNV
                     ALPMRRAASRDVRVGGVRFRAWCPPHALHPHLGIHHPLRIDVVDTWAKRGVIGGAYHV
                     WHPEGRAFAAPPLTRVEAAARRAQRFTIEGPSPWPLRARHVEPHPEQPYTLDLRRVDA
                     GLPMPRAEDWVVKE"
     gene            complement(9601..9966)
                     /locus_tag="VMZ53_23875"
     CDS             complement(9601..9966)
                     /locus_tag="VMZ53_23875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31574.1"
                     /translation="MSGVLTGRIENTVDATRGLPLADRVLARKIELEDALADLGPHDL
                     YEREAIQEALHSLYFIMPGDIAHPSPIVGRALSEWLERNKHVGLDIIRRQQAERLARM
                     IRNGEAPPECPNHSCDEAA"
     gene            10136..11146
                     /gene="argC"
                     /locus_tag="VMZ53_23880"
     CDS             10136..11146
                     /gene="argC"
                     /locus_tag="VMZ53_23880"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HUS31575.1"
                     /translation="MQRPRIAVIGASGYSGIELVRLLLSHDGVELVAAGSDKWAGRPV
                     EGAQQSHIGALSYAAHDVAASASADVFMLATPAEVSHELAPKLLARNARVVDLSGAFR
                     LRDAAAYATHYGFEHRHAGLLAEAVYGLPELDRSRQRDVRLIANPGCYATAIQLALSP
                     IRRLSGSQRFIVDAMSGVTGAGRKAVEEFSFAEIASDVRAYRVLRHQHTPEIEQQLQE
                     AVTFVPHLLPIKRGILATCHAQLVDGTTADDVARAFGMFEGERFIELAKSADDVAIKD
                     VVGTNKCRIGFSVGARGELVVISAIDNLVKGAAGQAVQNLNLMLGLPESAGLAGLRSF
                     HP"
     gene            11143..13269
                     /gene="argJ"
                     /locus_tag="VMZ53_23885"
     CDS             11143..13269
                     /gene="argJ"
                     /locus_tag="VMZ53_23885"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HUS31576.1"
                     /translation="MKVPIGFRFSGAHAGLKPVRRDVALIVSDVPAVAAGCFTQNLAA
                     AAPILDARPRVPSAAIRAVLVNSGNANAMTGPAGVADVVAIREALGTALGVGADAVLT
                     ASTGMIGVRLAASKVIGAVPALVTALTVVPEPAAEAILTTDTRTKLAFRTLELGGREV
                     TISAIAKGSGMIAPQLATMIAIVTTDAAISPAMLDRALRDAIDPSFHCLVVDGDMSTN
                     DTIIALANGQARNARIEAAGAELAAFTAALTSLCTELARDIASDGEGATRLLEVAVTG
                     APDVAVARDIARSIAASSLVKAAIFGADPNWGRVLATVGARAGSQHYAIDPHRTTVAI
                     QGVTVFAKGEPALTDAPALRSKMRAPEVRVEVELATGGGGTATAWGCDLGYDYVKLNA
                     DYSSLIVQTADGGLAKDDRLTNYSPSFKRKLIVEALSYISRFSGTRCVIKFGGSALSK
                     PALEQAFCDDIKLLRSVGLQPIVVHGGSAEVTRTLERLGHPTPEFVEGVRVTSPSDLK
                     VVDMALGSINSHLVSLLNLDGAHALGISGKDGALFRAKKKVSETGRDLGLVGEISQVN
                     GSVIEMLLNQGYVPVISPIALGEDGQGYNISADEAASAIAIALKAQKLLYLIDAPGIT
                     EHGELVTDLSVADLERKLATAESGMRVKLGAMIDALRGGVGRVHVIDGRTPHNLIAEL
                     FTDRGVGSLVTLDGDLAAAATLGGAQ"
     gene            13266..13712
                     /locus_tag="VMZ53_23890"
     CDS             13266..13712
                     /locus_tag="VMZ53_23890"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31577.1"
                     /translation="MSAQTRVRRSEIRTLITSQQVATQEELRSLLADRGHDVTQATLS
                     RDLAQLGARRTAQPTGGTYYELPDVPRAATPLLADLVRTIDDNGVLVVVHTTSGAAMV
                     VAGVLDRARLPEVLGTIAGDDAIFIAPARGVSTTRVIKKLRDLVAR"
     gene            complement(13709..14251)
                     /locus_tag="VMZ53_23895"
     CDS             complement(13709..14251)
                     /locus_tag="VMZ53_23895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31578.1"
                     /translation="MRRVAVVLVALAVRSAAADDGIALRVERDVGTQHEPYLRLDPAL
                     TSSLEGLTQETERHTTAITLGETRIILEGIASQNVDSDTRIHEDVESRGWRAAVRLTR
                     KLGPVQLEAWGAVENGQTRYGGGTYRDVGVALVKRFSLSRWMQAWIALSVNNRQWLDK
                     PPDGEANATTFMLSLGTTFR"
     gene            complement(14286..14657)
                     /locus_tag="VMZ53_23900"
     CDS             complement(14286..14657)
                     /locus_tag="VMZ53_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31579.1"
                     /translation="MRTSILFVVSALALGACGGDDGAEHSGVSTSKQLKDLSADERMQ
                     ECEWGVSYQGGAGHVTMCSDNVTVTVDTVPECVDAMDQYAASGCTATVAELEACTRAI
                     AADPCSFGGDACGPVFACLGQ"
     gene            complement(14721..15182)
                     /locus_tag="VMZ53_23905"
     CDS             complement(14721..15182)
                     /locus_tag="VMZ53_23905"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HUS31580.1"
                     /translation="MVGNIEPMTTINDARLREILTGSPTVAVLGIHREPEKAAFYVPE
                     YLKDEGYRVIGVNPQFRDEEILGEKVRESLSEIKEPIDLVDVFRRAEAIPAHVEDILA
                     MNPRPKYVWFQLGIKNDEAAKQLEDAGITVIQNRCTLADHQRLGLGKPARR"
     gene            15334..16266
                     /locus_tag="VMZ53_23910"
     CDS             15334..16266
                     /locus_tag="VMZ53_23910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31581.1"
                     /translation="MATPSFRKVIEATRSNAPGDLGCVIDTALTSEAIAVAFDARVGT
                     TIVVVTRAFVGNCPALSKLGSDRYVATVGGGSLVTDRKQSVLGSSQWEWAHDYLLTNP
                     LAIAAELPAKRVLAVAQPDPLDGWVAIEALEPTTIEKSLNELAARWRSEGKMQLGTKL
                     KTSRAGAQVVARLDKPDVDDMIALITDLTRNADAPPAQAPPAFACPPPSDLVKSCTQG
                     TAFVVTSTKRALESMTKTELALLIDNGDVAGLRASRDAALVLRAGDVVLGIDSHKVGS
                     PEQLQLLIAKAGPTAALAIRRDGVDTTVTLSESP"
     gene            16316..17629
                     /locus_tag="VMZ53_23915"
     CDS             16316..17629
                     /locus_tag="VMZ53_23915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31582.1"
                     /translation="MRSILIAVLLVAACGSDKNEKRAEPTRVEDRASVPPAPPPSAGS
                     AAGSSAGSPVATTPEPVKQADPVADTAPPAGGHDFTPEGKALLAVGACGGGAVPEGIP
                     QTLVDKHCSVITKAQDDYANGWLKKASPFFEQKVPKDLPKKVLYPFAGGDLSTALTVY
                     PDADEITTISLEPPGDPRTLGVLKGKALENALGKVEYELKFLYRVNFSNTLNMIEAMR
                     GGQLPTQLIFGLSALKIHGYELVALRYFRLNDDGTIHYLDDADLKKAPDPAKGDAQQR
                     NAVFSNCEVQFKKPGGRIQVWRHMRHNLGDDTMKGIGPGLKEDPRIVKHLEAKGQISG
                     MTKAASYLLSWDSFKIIRKVLLDHVVWMVSDATGVAPKWGKDAGFEYETYGLFEEPHI
                     GAGNNIAKNWKAEWAAQEKRELPFRFGYYDKKLRNHLVIMRKKSS"
     gene            17761..18741
                     /locus_tag="VMZ53_23920"
     CDS             17761..18741
                     /locus_tag="VMZ53_23920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUS31583.1"
                     /translation="MQLHEGRPFVAAGVVELWHATCWQVRHARPVEEVTVVAPTPSRS
                     RKLKRALTTGPQRLVRAVITPAERGPRFWAGAGTGTAVVVLACIALSRLGGNTTPSSL
                     AAFDASEPSEAPVVPAQSTSNEVEPPVPDLAALHPIPLEKDTPLDELYPSLLDWMHPV
                     AGTERVLPEYSTGTFGAERGGATHHECGAGHCGVDLGGPRGRAIVAVADGVVVHVERS
                     ELGRDGRSGRYVRLEHDDGTLTAYMHLDAIIDGLQVGDHVDGGQQIGTLGATAVYSAA
                     PHLHFSLEIPNVPGTHGDNVNTHYVDPAPFLVRASILDRAERRHPTKPAF"
BASE COUNT         3237 a         6243 c         6262 g         3030 t
ORIGIN      
        1 tcgggcgcct gcacgagctc gagccccatc gtgcgcgcga gaaagctgtg ctcgaagtac
       61 gccgagttgt acggaccggg cgtgaggacc acggcgtacg tctcgccatc aggagccggc
      121 gacaccgaac gcagcgtctc cgcgaggcgc gtcggatagt gatcgacgcg atgcacgcgc
      181 gctgcatcga acacgcgcgg gaacatgcgc ttgctcagct ggcggttctc catcacgtac
      241 gacacaccgc tcggcgtgcg caggttgtcc tcgagcacgc gccacgtgcc cgccggatcg
      301 cgaatgagat cgatgccgct gatgtggatg cggacgccgc ccggtggctt gaggccgcgc
      361 aggttcgcgc gatacgccgc ggcgccgagg atcatctcgg cgggcacgat gccggccgcg
      421 aggatcttct gctcgccgta gatatcgtcg aggaacatgc cgagcgcgcg cagccgctgc
      481 tgcaggcctc gctcgagtcg cgtccaatct gcgccggcga tcaaacgcgg cagcaaacag
      541 aacggaaaga tcttctcggt gccgcgtgca tctccataga ccgagaacgt cacaccctgg
      601 cggagcagcg agtgctcggc gagtgtctgc gcgcgcgcga gctcgtcggt ccccatcttc
      661 gcgagcagct gcgcgacgcg cgcgaacggc gcacgcggcg acccgctgtc gaacatctcg
      721 tcgtaggtcc ccggcagagc ggtgtagctg ccaaagaggc cacctccttg gctctggctc
      781 tgactctgca acatcaaaag catcgtaccc tcggacggac cttccatgat cgtgcacaag
      841 ccaccaactc cgtggaccgc gcgcccgcaa atcccgacgg tgtttctcgc gggcagcatc
      901 gagatgggct ccgcgccgga ctggcagagc gagctggtcc gcgagctcgg tgtcgacgca
      961 acatttctcg atccgcggcg cgccgagtgg gactcgagct ggcggcaatc gatcgacgag
     1021 ccgcagtttc gcggccaggt cgagtgggag ctcgacggac tcgagcgcgc tgatgtgatc
     1081 gcgatgtggt tcgtcgccga gacgaagtca ccgatcacgc tgctcgagct cggccttcac
     1141 gcgcgaagcg ggaagctcgt agtcggctgc ccacacggct tctggcggcg cggcaacatc
     1201 gaggtcgtgt gcgcgcgcta cggcgtgccg ctgtctgcgg aatgggacgc gttcgttggc
     1261 tcagtgagcc agacgatcat ggacacgcga cgtcgagagt gaactcaccg gcggtgccgt
     1321 cgggtccgtc gacgacgaag aagtacgtct cgccggcggt tccctcgaac gtgacgtctg
     1381 ggccaacgcc aatgcaatca tcgatcttgc atgcgccctc gggtgacgcg cgcaggactg
     1441 cgatcgagag ctcggggctc ggcgcggcga ggtgcgcggt gaccgggccg gactccgccg
     1501 gtgtgaatcg gaaaaagcgc tcgctgcccg tcgtgaatga atcggcacag ctgaacagat
     1561 cgaatcgcgc aggtccgccg cgtgttgtcc caggcgtcga tccacacgtc acgtttgtcg
     1621 cgcctcgaca cagtccaccc gtactgcaga cggcggttgc agcgcagtct gggtcagcac
     1681 agttctgaag gccgtcgagg tcattgtccc agtagtcgcc acacacgagc tcttgcgtcg
     1741 cggtgctacc cagatcgagg cgctggatca cgccgaaagc tccggtgaag gcgacggagt
     1801 gtccactgac ggagagactg gccaactggc gcgacgttcc cgactcgatg cggatccagg
     1861 tggcaccgtc ccaccgcagg agcattccga gatcgccgga cgcgtacacg ttgcctggcg
     1921 aaccagcgag tgcgtgcaat gtcgtcggcg cgcgcgtcgc gatccatgcc gtaccgtgcc
     1981 gctggacgat gtcgcctccc acggcgatca cttcgtcgcc accggtccac actgcgtaga
     2041 gcggaggcgc gctcgaggct gcagcacgga cgatcgacca cgccgaaccg tcgtagtgaa
     2101 gaatcacgcc cgcgtcaccc accgcgaaga cattcgcagt gctggtgccc catacgctga
     2161 agaggccacc gacgccgggc ggcacggtct gggccgacca cgtagagccg ttgaagtgga
     2221 gaattacgcc tccgttcgtg ccggcgccga ccgtgaagag atcgttgtag ctcgtgcccc
     2281 agatgccgtt gagcgtcatc gtattgtcgg agtgcacggc ggaccaagcg ggcgccgcga
     2341 cgctggtcct gatgacgccg cgttgaccga cgacaacggt gggaccgcca gccgtgctcc
     2401 agaccgcgtt cagcggctcg ccgcccaggt agtcctggac ccagacgttg ttgttgttgg
     2461 cgccaacgta ctcgtagatc acgccttgcg tacccactgc gccgacggtg tagagctcgt
     2521 cctcggtgag cccgaaaccg ccggcgtagt tcgcgcccgg ctttggaaga tacgactcga
     2581 tctggatcgc cgtcgaccgc acgattcggc cgttcgcgcc gacggcaaac acgttgtttg
     2641 catcagttcc gaacacggcg cgaagatcct cgacggcatg tgacgtgtcg gcgcgcgcgg
     2701 gccaggaaga gaagccggtg gtcttgtgaa agattgcacc gtcacttccg actgcgtaca
     2761 cgttggacgg agtgtgctcc cagatgccaa acaggttccc ggtcgtgtag acgggcgtgc
     2821 aggtgacggt gctgcagctg agctggtcga cgttctgata aatgatgacg ctgccgtcat
     2881 tgcgccccgc catccagacg tcgtttgcag ccttgcccga cagcgcgcgg atcgggccgc
     2941 ccgccgcctc gctgaatgcg atcgtccatg actggtccgg cttgtagtgc cgcaccatcg
     3001 cgccgagatg gttcgatgtg aagccagcga ccaatacgtc ggtcgcattc gcccacaccg
     3061 cgagcagctc ttcgtccccc ggcgcagcag cgttgacgag cttcgtccag ctgcctccgt
     3121 cgaagaacag caccgtgtcg ttcgttccga ctgcgaacgc gcgggcgggt gatacgacca
     3181 tcaccgcgtt cagcctttcc gttgtacccg tcgtcatcgg agtccacgcg acgccgttcc
     3241 actgcaggat agtgccgttc gcgccgacgg cgagcgcgaa gctcgagctg ctgcccgaga
     3301 tgcccatcag atccgccgtc gtgcccgaca ccatcggaga ccagccggcg ccgtcgtagt
     3361 gaaggatcgt accgccggtg ccgacggcaa acatatcgtt cgcacccgcg ccccacacag
     3421 cggtgagatc cgcgctcgtt cccgacggca tctgggtcca gccgatgcgg tcgcatcccg
     3481 acacgtcgtt cgtgcacgtg gtcgcgcagg tgatgcgtcc gacgtcgaaa ccgaaatcgc
     3541 cgcaatggtc cgccggcatc gcgaggtcgc aagcttcctg cggatccacc tcgccgtcgc
     3601 cgcagattcc gccggagcac gtcgacgtct ccggaaagca cgtcgaagag cacgtgagac
     3661 cttcaggtcc gtagtagcca aagtcgacgc agtcgagcga gccgagatcg aggccatcgc
     3721 actgctcgcc tggatcgacc ttcccatcgc cgcacgtctt ggggacgcac agcgcttctc
     3781 gacagaccgt accgctcgga cacacgcgac cgtcgtcgca cacgaccgag ctcgacgaga
     3841 tgcacccgcc gacgagcatc acgatcgccc acgtcacgat cgcccacgct cgcatgacgt
     3901 gagtctatca ggtctcgtcg atcaaccgtc cgacgaggac atcacgagcg cgatcagcga
     3961 ccacacgagg ccaagcaccg tggccacggc catgaagaag ccgccgatcg acgcgtgaaa
     4021 cgcgagatcg aagatgtgcc cgaagaattt gtacgcgagg tagccgaacg agaacgtgcc
     4081 gaccactgcc gcagccgtgt caccttcggt cttgctcagc ccgacgtagg tcggcagcgc
     4141 gaacgcgccg atcacgagcc acacgtgcca gccctcgctg aagtgccgca gatcgaacag
     4201 cgacaggtgg ccagcgaccg gcaggaagca gccgatgagg ccgagcaagc cgaatacaaa
     4261 gatcaggcgc ttcatcggcc tggccttagc ggagctggct cgggtcctgc ggcgtgccct
     4321 tcatgaccgc gacgatgatc caaccgatga cgccgagcag gccgccgagc acgagcccca
     4381 cgccgccgag gcctttgttc ttgccgatga gccaaccaac gagagcgccg ataacgagac
     4441 cgatgataat tgccatgtgt tttgtcctcc tgctaacgag ccgcagggtc gcgcgcggtc
     4501 gcacactttc ggcgaagtgc gcgtaacggt tacgcgatac cgtcgcggcg taggtcgatc
     4561 gtgaacgggc gcgcttcacg ccagaacgtc ggggcggagg catcgccctc gtgggtcacg
     4621 atcttgatgc gttcttggcg gcgctcgagc gcttcattgg tgtcgcgcgg aagaccggag
     4681 taggggccgc cgcctgggcg ccatgcccac agctcgacgc gctggctgcg gcccgcccac
     4741 gcccactcga tcgcgagcgg atcgatgacc ggcaggcccg gatgcaggcc gggcgacggc
     4801 tgatagatgc ggcggcgcac gccgatcgcg cggacgtgat cgccgacgag acgaaggtgc
     4861 acccatttgc cgccgatgat gacgcgatcg gggcccgctc catcgagaga aacttcccag
     4921 cgctgcgtcg atgcatcgac ccaccgcgcg gtcgcgcgct cctgcgatgc aacatcgccg
     4981 acgagcggcc agaactcgag cgcggggcgc agcgtgagcg tcgcgtcgcc gagccggcac
     5041 gtgatgccgg gggagcggaa tgcttcgagc tcgcggcgca gctgcggcgg aaggccgaga
     5101 ccatgctcgt cgagatcacc gagcaccatc cgcaggtcgc gatggagcgc ggcgggcaat
     5161 gcgaagcgat cgtgcagctc gtcatgccag tcgacgagcg gcgcgcggta gtgcgagacg
     5221 acgaggcgcg cgacgatcga gcggaacaga gcggcaagcg ccgcgagcat gccgggccga
     5281 tcgggcatgc ggatcgcgcg cagctctacg acgcccatct tgccgtggcg cggaccgtgc
     5341 gccggcacgt gcgggctcca cagcttctcg acgttgagct ccgcgcggtg agagttacct
     5401 gcgctgtcga cgagcagtgg cgagagcccg gcctgcattt ggtcggggcc cagctcgccg
     5461 cgatccgcga ggtgctcgag ccaaccgagc gtgacggcga gctcgtccca gcgctcgcgc
     5521 gcgccttcgt cgggacgcgg accctgcgat gccgaaccca cgcattcgct cgcgaaccag
     5581 tacgacagcg atgggtggtt gttcacgtag cgaacgagcg cgggcagcac gtgcggatag
     5641 cgaatgaacg gcgacgtgtc gggacgcgag ccaccgagcg tgatctgacc accgccgcca
     5701 gagtcggcga catcgccgtt gaagcgaaac cgcgtcggcg agaggccggc agtctcggcg
     5761 gcgcgccaca cctgctgggc gtgcgcgtag aacgtgcgcg tgttcgccgc gggcgccatg
     5821 ttcacttcga cgacgcctgg atctggcgtc accgtcagcc agtgatcgct gccgaggtcg
     5881 gtcggcggcg gttgcggttc ggcgtcgacc gcggcgcgct cgccttgatc gacgatgccg
     5941 atgatctcgt cgcgccagcg tacaccgaat gcgaagcgag gcttctcctc gccgggaaac
     6001 gtccgcccga cgacacgcgt gacggatgcg cccgagagct ggttcgcgat cgcggtcgcc
     6061 acggcgtgag cgcgtgcgag cttgtcgccg ccctctgctg cgccggtcca ggcgggctcg
     6121 acggactcgg cgcgcgtgaa cgttggctcg gcaccgaccc agacatcgac gcctcggcgc
     6181 gcgatcagct cgtcgtgcat ctcgaggctg gcggccaggt cgtcggccat ggctactcct
     6241 tgacgaccca gtcttccgcg cgcggcatcg ggagccccgc gtcgacgcga cggagatcga
     6301 gcgtgtacgg ctgctcgggg tgcggctcga cgtgacgcgc acgcagcggc cacggcgagg
     6361 gaccctcgat cgtgaagcgt tgggcgcgcc gtgcagcggc ctcgacgcgg gtgagcggcg
     6421 gtgcagcgaa cgcgcggcct tcgggatgcc acacgtgata cgcgcctccg atcacgccgc
     6481 gcttcgccca ggtgtcgacc acatcgatgc gcagcggatg gtgaatgccg agatgcggat
     6541 gcagcgcgtg gggagggcac cacgcgcgga agcgaacgcc accgacgcgc acatcgcgcg
     6601 acgcggcacg ccgcatcggt agcgcgacgt tgttgacggc gatcagatag cgctcttcgt
     6661 cgaggccgtg cgcgcgaatc tcgatgcgct ccatcgatga atcgacgaag cgagcggtgc
     6721 cgctctgcgt cgcttcttcg ccgagcacgt gccacggctc gatcgcgttg cggacttcga
     6781 cacgaccggg gccgacatcg agcgagccga cgagcgggca gcgcagctcc acgaacggct
     6841 tgtacgcttc gctcggcagc gcgacgccgc gtgccgcgag atagccgagg acgtcctcga
     6901 agtcgcgcca catgtaatag ggcaggagga agcgatcgtg gagctcggcg ccccagcgta
     6961 cgagcgcgtg cttgtatggc tcctgcgtga acgcagcgac gagcgcgcgc gtgagcacga
     7021 cctgcgccgc cgtcatgcgc ggatgcgggg gcatctcgaa tgcgcgcagc tcgacgaggc
     7081 cctggcggcc gaacggcgtg tgcgggtcga acagcttgtc gatcgacagc tccgcgcgat
     7141 gcgtcgagcc ggccacatcg acgaggagat ggcgcagcag cgcgtcgact tgccagaccg
     7201 gcgtgccggg cgtgaacagc tgcggcagcg cgatctcgag ctcgtacagt gcgtcgtggc
     7261 gagcttcgtc gacgcgcggt gcttgcgacg tcgggccgac gaacagaccc gtgaacatgt
     7321 acgacagcga cggatgatgc tgcacgaacg agatcaggct cgcgagcagc tcgggcttcg
     7381 tgagaaacgg cgatgccatc ggctgcgatg caccgagcgt gatgtgattg ccgccgcccg
     7441 agccggccat gcgaccgtcg agcatgtagc gctctgcggt gagaccgacg gacagtgcgg
     7501 cgtcgaatac ggtcgcgtgc agctcggccg cctcgcgtcc ggaccacgac ggcgggaggt
     7561 tcacctcgag cacaccagga tcgggcgtga cggcgaaccg gcgcagattc ggtgacggcg
     7621 gcggaccgta gccttcgagg ttgacgtcga agcccgtggc gacgcgcgct tcgtcgagcg
     7681 cggcgatcag ctcgcagaaa tcggagaacc gcgtcatcgg cggcaagaaa atccagagct
     7741 cgccattgcg cggctcgacc gcgagcgcgg tgcggatgcc aatcccggac tcgctgcgct
     7801 ggacgcggcg cgcaggcgac tcgcgacgaa gcgcgtcttg cgtgtcgtcg tcggtgtcgt
     7861 cgcgtcgcgg atcggcaacg tttggatcgt cagcccacag cggaggcgcc acggcctccg
     7921 gcaagctgcc gagcggaagc ctcagaccga ccgggctgtc gccgggcaac aggaacagat
     7981 gcccgcggcg aaacttccag gcttcggtga gccagcctgc agcgccacgc gcgagcggca
     8041 acacccagcc gacggcggag ccgacacctt gcgcgaagat cttcgcgagt cgttgtcgct
     8101 cttccggatc gtcgagacct gccttggtcg gatcgacgtc gggtggcagc accgcttcgc
     8161 tgcgcatcac ctcccacgga tcttcgtagg ccggatgcag gagctctcgc atgccgagga
     8221 tctcggcgag cgcttcgcca tagtgcttcg catccgcgat cgatccggtc gcacgcagct
     8281 cgcgatccgg ccacagcgca acgccatcgc gacgcgcgat gatgtcgagc gcccagcgcg
     8341 gcaggctctc gcccggatag tgcttgccca tccgatgcag cacgacgccg gtaggcgcga
     8401 gcctgtcgcg aagctcgtcg gcgagcgcgc ggcccttctg ccacttgtcc gcgccgagtg
     8461 cgccgccttg ccactcgacg gcctcgacgc catcacgcgc ggtgaacgtg ggctcgccac
     8521 cgacggtgag ctcgacgccg gcctgctgca gcagctcgtc tgcgcgatca ctggcggaga
     8581 gcagctctcc ccagatctcg tccgtgaatg gcgccgtcgg ccgcgcctcg tgcccgagcc
     8641 tcgcgatcgt cgtcgagaac tcgacgctcg cagccgcgac gttgctcgta ccgtcgagcg
     8701 gtgcggcatg cgcgggtgtc gccgtcgcag cgagcgggat gtgtccctcg ccggtgagca
     8761 ggccactcgt cccgtcgagc ccgatccatc ccgcacccgg tacgtatgcc tcggcccacg
     8821 catgcaggtc gacgacgtcg cgcgacacgc ctttgggctc gttcgggatc atgccctcgt
     8881 cggtgagctg gatgaggtaa cccgagacga agcgagcggc gattccgcgt gcgcgcaaga
     8941 gcgcgacgag cagcaccgca ctgtcgcgac agctgccgcg gccgtttgcg agtgtctctt
     9001 caggcgtcca gacgccggtc tcgtcgcgga tcacgtagtt gacgcgctcg cgaaccaagc
     9061 gattcgcctc gacgagcatg ccgacggtgt ctccactcgc gggcaccttt gcgagcagct
     9121 cgttcgcgag cggccccatg cggtatgcgg catcgccgcg atccaagaac acgccgagct
     9181 cggtctcgag cgcatcggga taacgaaacg gcagctgctt cgcgcggtcg tcgacgaaaa
     9241 agtcgaacgg attcaccggc ccgacgtcga ccgcgatctc gacgacgaca tcgagctcgc
     9301 gaatctcttg cccgctcttg aacgtgagcc gagcgactct gttcccgtgc ggatcgcgct
     9361 gccagtgcag gcgatgctcg ggtgaaacgg cgagcttgta gctttcgatg cgcgcacgcg
     9421 cgtgatcgga cggcctcaat cgaaccaagt gtggtccgag gagcgcgggt cggggatagt
     9481 gataacgact gcgatgctgg acgagcacgc gcacggggaa cgtggtgtac cacgccacca
     9541 cccgggccgc gttttccagc gctgtgcgtt cgcgctcgcc gcgcggcttc gcccgtcagg
     9601 ttacgcggct tcgtcgcacg agtgattcgg gcactcgggc ggcgcttcgc cgttgcggat
     9661 catgcgagcg aggcgctcgg cctgctgccg acgaataatg tcgagcccga cgtgcttgtt
     9721 gcgctcgagc cactcgctca gcgcccgccc gacgatcggc gacggatgag cgatgtcacc
     9781 gggcatgatg aagtagagcg agtgcagcgc ttcctggatg gcctcgcgct cgtaaagatc
     9841 gtgcgggccc aggtcagcaa gcgcgtcttc cagctcgatc ttgcgcgcta gcacgcgatc
     9901 cgccagcggc aggccgcgcg tcgcgtcgac ggtgttctcg atgcgaccag tcaaaactcc
     9961 gctcatgacg aacacaggta gagcacctcg tgtgccactc cacgccgacg atcaccgtgc
    10021 accgccgctc gtgcgccggc gtcgcagcgg gccgcccgat cacgcctcgc cgcgtgtgat
    10081 cggccacgtt ggcccttgca tatctgcata gaagacgcat attcatgcag aaactatgca
    10141 gcgccctcgc atcgcagtca tcggcgcctc gggctactcg ggcatcgagc tggtgcgcct
    10201 gcttctgtcg cacgacggcg tggagcttgt cgcggcgggc agcgacaaat gggcgggccg
    10261 gcctgtcgag ggcgcgcaac agtcccatat cggcgcgctg tcgtacgccg cgcacgatgt
    10321 cgctgcatcg gcgagcgcgg acgtcttcat gctcgcgacg cctgccgagg tctcgcacga
    10381 gctcgcgccg aagctgctcg cgcgcaacgc acgcgtcgtt gatctgtccg gcgcgtttcg
    10441 cctgcgcgac gcagctgcgt acgcgacgca ctacggcttc gagcatcgcc atgctggcct
    10501 tctcgcggaa gccgtctacg gtctgcccga gctcgatcga tcgcgccaac gcgacgtgcg
    10561 gctcatcgcg aacccgggct gctacgcgac cgcgatccaa ctcgcgctct cgccgatcag
    10621 gcgcctatct gggtcgcagc gattcatcgt cgacgcgatg tcgggcgtga ccggcgctgg
    10681 acgcaaagcc gtcgaagagt tttcgttcgc cgagatcgcg agcgacgtcc gcgcctatcg
    10741 cgtgctccgt catcagcaca cgcccgagat cgaacagcag ctgcaagagg cagtcacgtt
    10801 cgtgccgcac ttgctgccga tcaagcgcgg catcctcgcg acgtgccatg cgcagcttgt
    10861 cgacggtacg acggccgacg atgtcgcgcg cgcgttcggt atgttcgaag gcgaacggtt
    10921 catcgagctc gcgaagagcg cagacgacgt cgcgatcaaa gatgtcgtcg gcacgaacaa
    10981 atgccgcatc ggcttcagcg tcggcgcgcg cggtgagctc gttgtcatca gcgcgatcga
    11041 caatctcgtg aagggtgcgg ccgggcaggc cgtgcagaac ttgaacctga tgctcgggct
    11101 gccggagagc gcagggcttg ccggcctgcg gagttttcat ccatgaaagt gccgatcggt
    11161 tttcgtttct cgggtgcgca cgcgggtctc aagccggtac ggcgcgacgt ggcgctgatc
    11221 gtttccgatg tgcccgccgt ggctgccggt tgcttcacgc agaacttggc tgccgcggcg
    11281 ccgattctcg atgcgcgacc acgcgtgccg agtgcggcca tccgcgccgt cctcgtcaac
    11341 agcggcaacg cgaatgcgat gacaggacca gcgggcgtgg cggacgttgt cgcaattcgc
    11401 gaggcgctcg gaacggcgct cggcgtcggc gcggatgccg tgctcaccgc gtcgaccggc
    11461 atgatcggtg tgcggctcgc cgcgagcaaa gtgatcggtg cagtgcccgc gctcgtgacc
    11521 gcgttgaccg tggtgcccga gcctgcggcc gaggcgatcc tgacgacgga cacgcgcacg
    11581 aagctcgcgt ttcggacgct cgagctcggc ggccgcgagg tgacgatcag cgcgatcgcg
    11641 aagggctcgg gcatgatcgc gccgcagctc gccacgatga tcgcgatcgt cacgaccgat
    11701 gcagcgatct cgccggcgat gctcgaccgc gcgctgcgcg acgcgatcga tccgagcttt
    11761 cattgcctcg tcgtcgacgg cgatatgtcg acgaacgaca ccatcatcgc gctcgcgaat
    11821 gggcaggcga ggaatgcgcg catcgaagcg gcgggtgcgg agctcgcggc ctttaccgcc
    11881 gcgttgacgt cgttgtgcac ggagctcgcg cgagatatcg cgagcgacgg tgaaggcgcg
    11941 acgcgcctgc tcgaagtcgc ggtcaccggt gcgcccgatg tggcggttgc gcgagacatc
    12001 gcgcgatcga tcgcggcgag cagcctcgtg aaagcggcga tcttcggcgc ggatccgaac
    12061 tggggccgcg tgctcgcgac ggtcggagcg cgcgccggtt cgcagcacta cgcgatcgat
    12121 ccccatcgca cgacggtggc cattcaaggc gtaacggtct tcgcgaaggg cgagcccgca
    12181 ttgacggatg cacctgcgtt gcgctcgaag atgcgtgcgc cggaagtgcg cgtcgaggtc
    12241 gagctcgcga ccggcggcgg cgggacagcg acggcgtggg gctgcgacct cggctatgac
    12301 tacgtgaagc tcaacgcgga ctactcgtca ctcatcgtgc agacggcgga tggtgggctc
    12361 gcgaaagacg atcggctcac gaactacagc ccgagcttca agcgcaagct catcgtcgag
    12421 gcgctgtcgt acatctcgcg cttttccggt acgcgttgcg tgatcaagtt cggcggcagc
    12481 gcgctgtcga agcccgcgct cgagcaagcg ttctgcgacg acatcaagct gctccgctcg
    12541 gtcggtctgc agccaatcgt cgtgcacggc ggctcggccg aggtcacgcg aacgctcgag
    12601 cggctagggc atccaacgcc ggagttcgtc gagggcgtgc gcgtaacgtc gccgagcgat
    12661 ctgaaggtcg tcgacatggc gctcgggtcg atcaactcgc acctcgtgtc gctgctgaac
    12721 ctcgacggcg cgcatgcgct cggtatctcc ggcaaagacg gcgcactgtt tcgcgcgaag
    12781 aagaaggtct cggagacggg ccgagacttg ggcctggtcg gcgagatttc gcaggtgaac
    12841 ggcagcgtga tcgagatgct gctcaaccag ggctacgtgc cggttatctc ccccatcgcg
    12901 ctcggcgaag acgggcaggg atacaacatc agcgccgacg aagcggcgag cgcgatcgcg
    12961 atagcgctga aggcgcagaa gctgctgtac ctcatcgacg cgccgggcat caccgagcac
    13021 ggcgagctcg tgacggatct gagcgtcgcc gatctcgagc gcaagctcgc gacggcggag
    13081 agcggcatgc gcgtgaagct cggcgcgatg atcgacgcgc tccgcggcgg cgtaggtcgc
    13141 gtgcacgtga tcgacgggcg cacaccgcac aacctgatcg ccgagctgtt caccgatcgc
    13201 ggtgttggct cgctcgtcac gctcgacggc gatctggccg cggcagccac gctcggaggt
    13261 gcgcaatgag cgcgcagacg agagtgcggc gctccgagat ccggacgctc atcacgagcc
    13321 agcaggtcgc gacgcaggaa gagctgcgct cgctgcttgc agaccgcggt cacgatgtca
    13381 cgcaagcgac gctgtctcgc gatctcgccc agctcggcgc tcgccgcact gcgcagccaa
    13441 ccggcggcac gtactacgag ctgcccgacg tgccgcgtgc ggccacacca ctgctcgccg
    13501 atctcgtgcg cacgatcgat gacaacggcg ttctcgtcgt cgtgcacacg acgtcaggtg
    13561 ctgcgatggt cgtcgcgggt gtgctcgatc gcgcgcggct gcctgaagtg ctcggcacga
    13621 tcgcaggtga cgacgcgatc ttcatcgcgc ccgctcgcgg tgtgtcgacg acgcgcgtga
    13681 tcaagaagct tcgcgatctc gtcgcgcgct agcgaaacgt cgttccgagc gacagcatga
    13741 atgtggtggc gttggcttct ccgtcgggag gcttgtcgag ccactgccgg ttgttcacgc
    13801 tcagcgcaat ccacgcctgc atccagcgcg agagtgagaa tctcttgacg agtgcaacgc
    13861 cgacatcgcg atacgtcccg ccgccatagc gagtctgccc gttctctacg gcgccccacg
    13921 cctcgagctg caccggcccg agcttgcgag tgaggcgcac cgccgcacgc cagccgcgac
    13981 tctcgacgtc ctcgtgaata cgcgtgtcgc tgtccacgtt ctgcgacgcg atgccctcga
    14041 ggatgatgcg cgtctcgccg agcgtgatgg cggtcgtgtg tcgctcggtc tcctgcgtga
    14101 gtccctcgag gctcgaggtc agcgctggat ccagccgcag atacggctcg tgctgcgtac
    14161 ctacgtcgcg ctcgacgcgc aatgcgatgc catcatctgc cgcggcggac cgcacggcca
    14221 acgcgacgag gacgacggca acgcgcctca catcgctcag actcgcgccg ccgcaaaagg
    14281 gttcgctact gacccaagca agcgaacacg ggaccgcagg cgtcgccgcc gaagctgcac
    14341 ggatccgcgg cgatcgcgcg tgtgcacgcc tcgagctctg caacggtcgc ggtgcatccg
    14401 gatgccgcgt actgatccat cgcatcgacg cattcaggaa cggtgtcgac cgtgaccgtc
    14461 acgttgtcgg agcacatcgt cacgtggccg gcgccgcctt gatacgacac gccccactcg
    14521 cattcctgca tccgttcgtc ggcggagagg tccttcagct gcttcgacgt cgacacgccg
    14581 gagtgctccg cgccatcgtc gccaccacac gcgccaagcg ccagggccga gaccacgaac
    14641 aagatagacg tacgcatcga aggctccctc cgtagtggaa gctcgattgt cgctcgaacc
    14701 gcgagggtcg cgcgagcttt ctaccgacgg gccggtttgc cgagacccag gcgctgatga
    14761 tcggcgagcg tgcagcggtt ttggatcacc gtgatgcccg cgtcttcgag ctgcttggcc
    14821 gcctcgtcgt tcttgatgcc gagctggaac cacacgtatt tcggccgcgg attcatcgcc
    14881 agaatgtctt cgacgtgggc gggaatcgcc tcggctcggc ggaagacatc gacgagatcg
    14941 atcggttctt tgatctcgct gaggctctcg cgcaccttct cgcccaagat ttcttcgtcc
    15001 ctgaactgtg gattgacgcc aatgacgcga tacccctcgt ccttcaagta ctccggcacg
    15061 tagaaggccg ccttctccgg ctcgcgatgg atccccaaca ccgctacggt cggcgacccc
    15121 gtcaagatct cccgcaagcg ggcgtcattg atggtggtca tgggttcgat gttaccaacc
    15181 atctgctatg gagaaagggt gacctcctgc gcaagggtga tgctctgcat ggcgctcgcc
    15241 gcgtgcggca cacgcacacc tactgcgcgc gaacgcgcac tccgcgagct tccggcgcaa
    15301 gcacaaataa ttgcagcggc cgacgggcca acgttggcca cgcctagctt tcgcaaagtc
    15361 atcgaggcga cacgctcgaa cgctcctggc gatctcggct gcgtgatcga tacggcgctg
    15421 acgagcgaag cgatcgcggt cgcgttcgat gcacgcgtcg gcacgacgat cgtcgtcgtc
    15481 acacgcgcgt tcgtgggcaa ttgccccgcg ctctcgaagc tcggcagcga tcgatacgtc
    15541 gcgacggtcg gcggcggttc gctcgtgacc gatcgcaagc agagtgtgct cggcagctcg
    15601 cagtgggaat gggcgcacga ctacctgctg acgaatccgc tcgcgatcgc ggcggagctt
    15661 ccggccaagc gcgtgctcgc ggtcgcgcaa ccggatccgc tcgatggctg ggtcgcgatc
    15721 gaggcgctcg aaccgacgac gatcgagaag tcgctgaacg agctcgcggc gcgctggcgc
    15781 agcgaaggca agatgcagct cggtacaaag ctcaagacgt cacgcgctgg agcgcaggtt
    15841 gttgctcgcc tcgacaagcc cgacgtcgat gacatgatcg cgctgatcac cgatctgacg
    15901 cgcaatgccg atgcaccgcc cgcgcaagcc ccgcctgcat tcgcgtgccc gccgccgagc
    15961 gatctcgtga agtcgtgcac gcagggaaca gcgtttgtcg ttacctcgac gaagcgagcg
    16021 ctcgagtcga tgacgaagac cgagctcgca ctgctcatcg acaacggtga cgttgccgga
    16081 ctgcgcgcct cgcgcgacgc cgctctcgtg ctgcgcgccg gcgatgtggt cctcggcatc
    16141 gatagccaca aggtgggttc gcccgagcaa ctgcaattgc tcatcgcgaa agcaggacct
    16201 acagcggctc ttgcaattcg ccgcgacggt gtcgacacga ccgtcacgct cagcgaatcg
    16261 ccgtagcgct tcatcgtgga gatttaccgg cggtcgttgc atagtccggc cgctcatgcg
    16321 gtccattctc attgccgtcc tgctcgtcgc tgcttgcggt agcgacaaga acgagaaacg
    16381 cgccgagccg acgagagtcg aggaccgcgc atcggttccg cctgcgccgc cgccaagcgc
    16441 cggatccgcg gcaggttcga gcgcgggctc tccggtcgcg acgacgccgg agccggtgaa
    16501 gcaggctgat ccggtcgccg acacggcgcc tcctgcggga ggtcacgact tcacgcccga
    16561 aggcaaagcg ttgctcgcgg tcggcgcatg cggtggcggc gcggtgcccg aaggcatccc
    16621 gcagacgctc gtcgacaagc actgcagcgt gatcacgaag gcgcaggatg actacgcgaa
    16681 cggctggctg aagaaggcgt caccgttctt cgagcagaaa gtcccgaagg atctgccaaa
    16741 gaaagtgctc tatccgttcg caggcggcga cctgtcgacg gcgctcaccg tgtatcccga
    16801 cgccgacgag atcacgacga tctcgctcga gccgcccggc gatccgcgca cgctcggcgt
    16861 gctcaagggc aaggcgctcg agaacgcgct cggcaaagtc gaatacgagc tgaagtttct
    16921 ctatcgcgtg aacttctcga acacgctcaa catgatcgag gccatgcgcg gcggtcagct
    16981 cccgacgcag ctcatcttcg ggctctccgc gctgaagatc cacggctacg agctcgtcgc
    17041 gctgcgctac ttcaggctca acgacgacgg cacgattcat tatctcgacg acgcggatct
    17101 caagaaggcg ccggatcccg cgaagggcga cgcgcagcag cgcaacgccg tgttctcgaa
    17161 ctgcgaggtc cagttcaaga agccgggcgg ccgcatccaa gtctggcgtc acatgcgcca
    17221 caacctcggt gacgacacga tgaagggcat cgggcccggg ctcaaagagg acccgcgcat
    17281 cgtcaagcac ctcgaggcga aggggcagat cagcggcatg acgaaggccg cgagctatct
    17341 cctgtcgtgg gactcgttca agatcatccg caaggtgctc ctcgatcacg tcgtctggat
    17401 ggtgtccgac gcgacgggag ttgcaccgaa gtggggcaag gacgcgggct tcgagtacga
    17461 gacgtacggc ctgttcgaag agccgcacat cggcgcgggc aacaacatcg cgaagaactg
    17521 gaaggccgaa tgggctgcgc aggagaagcg cgagctgcca ttccgcttcg gttactacga
    17581 caagaagctg cggaaccacc tcgtgatcat gcggaagaag tcctcgtgat ccgtcgacgg
    17641 tgtacgacac gtcggtacag ctgattccga gaagaatgat cccgcgctcg cgatcgcgcg
    17701 cgaagtcgat ccgctagcat cgatcgcatg ggtgctgctt gtccgcgttg cgggcaaact
    17761 gtgcagttgc acgaagggcg tcccttcgtc gcggctggcg tcgtcgagct gtggcatgcg
    17821 acgtgttggc aagtgcgcca cgcgcggccc gtcgaagaag tcaccgtcgt cgcgccgacg
    17881 ccatcgcgct cgcgcaagct gaagcgcgcg ctgacgaccg gaccgcagcg tctcgttcgc
    17941 gccgtgatca caccggccga gcgcggtcct cggttttggg cgggcgcagg aaccggcacc
    18001 gccgtcgtcg tgctcgcgtg catcgcgctg tcgcggcttg gtggcaatac aacgccgtcc
    18061 tcgctcgccg ccttcgacgc gtcggagccg agcgaagccc cggtggtccc ggcccaatcg
    18121 acgagcaacg aagtcgagcc accggtgcca gaccttgccg cgctccaccc gattccgctc
    18181 gagaaggaca cgccgctcga cgagctgtat ccgtcgctgc tcgactggat gcatccggtc
    18241 gcaggcaccg agcgcgtgct tccggaatac tcgacgggca ccttcggcgc agagcgcgga
    18301 ggcgcaacac atcacgagtg tggggcgggg cattgcggcg tcgacctcgg tggcccgcgc
    18361 gggcgtgcga tcgtcgcagt cgccgacggc gtcgtcgttc atgtcgagcg ctccgagctc
    18421 ggtcgcgatg gccgcagcgg ccgctacgta cgtctcgagc acgacgacgg gacgctgacg
    18481 gcgtacatgc atctcgatgc gatcatcgat ggcctgcaag tcggcgatca cgtcgacggt
    18541 ggtcagcaga tcggcacgct cggcgcgacc gccgtgtact ccgctgcgcc gcacctgcac
    18601 ttctcgctcg agattccaaa cgtgcccggc acgcacggcg acaacgtgaa cacgcactac
    18661 gtcgacccgg cgccattcct cgtgcgggca tcgatcctcg accgcgccga acgtcgccat
    18721 ccgaccaagc cggccttctg atctatagtg ggggcatgcc ctcccgcgag ga
//
LOCUS       DATLBZ010000471         4448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10736573, whole genome shotgun sequence.
ACCESSION   DATLBZ010000471 DATLBZ010000000
VERSION     DATLBZ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10736573"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1103
                     /gene="arsM"
                     /locus_tag="VMZ53_23925"
     CDS             204..1103
                     /gene="arsM"
                     /locus_tag="VMZ53_23925"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HUS31584.1"
                     /translation="MKPDEIRSIVRDHYGAIARGRATGTSTAGCCMRSDAQASTPGCC
                     DTQDALTQDPETLGYRGDEPAPDGANLGLGCGNPTVIASLRPGERVLDLGSGGGFDCF
                     IAAHQVGETGRVIGVDMTADMVSLARANARKVNATNVEFRLGEIEHLPVADSSIDVIL
                     SNCVINLSPDKRAVFAEAMRVLAPGGRLAIADIVATRAIPTQMQNDVAALCGCVAGAA
                     LLDDVRAMLVDVGFGDVEVSVTPRSAEILGGWMPGIDQYVASATIEARKPGAMHARAG
                     GATSATAGEPAKQRSCCGPECCA"
     gene            1076..1696
                     /locus_tag="VMZ53_23930"
     CDS             1076..1696
                     /locus_tag="VMZ53_23930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS31585.1"
                     /translation="MLRTGVLRMNASPEQLRSASPAGELWRSVDAQLRPFIAKRVDAA
                     DVDDVLQDVFVRMQRGLGQVREEERFSGWLFQVARTAIAEHGRARARHPLPKVDVVED
                     APAGPSTDDDRVASRALSGCVAVFVARLATPYREAVTLVELEGLTAREAAEIVGISVS
                     GMKSRVQRGRQQLREMMEACCEIALDARGKVTDFAPRVDRCACGST"
     gene            complement(1689..2384)
                     /gene="gloB"
                     /locus_tag="VMZ53_23935"
     CDS             complement(1689..2384)
                     /gene="gloB"
                     /locus_tag="VMZ53_23935"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HUS31586.1"
                     /translation="MRVLAIPCLKDNYAYLVIEGTYAVIVDPSEHTPVRTAIEREGVT
                     LGGIWLTHHHWDHVGGVEKLVEETPGLVVVAHAADAPRAPKVTHVVEDGETIQCGPLR
                     TKIIHNPGHTLGAISYLIDDCVFTGDTLFGAGCGRVFEGTMEMMHASLQKLAALPPKT
                     KVYFGHEYTASNLRYAMHAEPDNAAMKQRAESLATPSTPSLIGLERETNPFLRAADAA
                     DFAARRGEKDSFK"
     gene            2434..3744
                     /locus_tag="VMZ53_23940"
     CDS             2434..3744
                     /locus_tag="VMZ53_23940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS31587.1"
                     /translation="MRILLASLVVALVACGPSPDDRTMGDDDMNTDCEGIACQVVQCE
                     KMGKPPTTISGTVYAPNGTLALYGANVYVPLVDPGPFAEGVTCTQCQDQLPGGAVANG
                     MSDTSGKFSVSGVPSGTDIPLFITIGKWRRKTVIPQVLPCQDNPLPNTVTSLPKNKTE
                     GDIPKIAIATGSCDALECLLRKIGVADSEFTADSGSGRIHLYSANGSPKIASSNTMLS
                     PATALWGDVNKMKNYDMMLFSCECGPYPENKSQPMMDNLKAYADMGGRVFLSHYHSVW
                     ISGEKSVPTHAPAVWPTVASCNIDEYQSGTGVIDQVNNPKGSAFAQWMTNVQGSTTPG
                     TVEINDSRQSCTSVDNTKAERWVYMKIGGTDYPQDFQFTTPQEVAKEARCGKVVFSDM
                     HVASGSTSSSTGFPSGCGAGAMSPQEKALAFMLFDIATCVGPIL"
     gene            3762..>4448
                     /locus_tag="VMZ53_23945"
     CDS             3762..>4448
                     /locus_tag="VMZ53_23945"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HUS31588.1"
                     /translation="MANMTACVLREHGGPEVLHLEELPLPEPGPGEVRVRIRAVALNH
                     LDIWVRRGGPAFKLEYPHRLGSDIAGEVDAIGAGVTGVTVGAKCVVQPGISCMRCTAC
                     LGGHDNLCRYYKILGENTQGGYAQVIVVPAVNIAPYPGDLDFPNAAASVLPFLTAWQM
                     VVHKARVQPGETVLVQGAGSGIGVAAIQIAKLFAARVITTASSEDKLARARALGADAG
                     IDYSTQDFATE"
BASE COUNT          746 a         1390 c         1543 g          769 t
ORIGIN      
        1 tgcgagagga gggcgacgat gcgcacgcgc gtctcgtcgg caagtgcgcg gaacagcttg
       61 gtcagctcgc gcacgcctgc ctcgtcgacc cgctcggcgg ctgatatcat agacatatgg
      121 atatccgtat gtcctgatac agtcaagctg cgtccttttg gcgagatgcc cgtcttccta
      181 gacaggagga caacatgagt gaaatgaagc ccgacgaaat ccgcagcatc gttcgtgacc
      241 actacggcgc cattgcgcgc ggaagggcga cggggaccag tacggccgga tgttgcatgc
      301 gaagcgacgc gcaagcgtcg acgcctggat gttgcgacac gcaggatgcg ctgacgcagg
      361 atccggagac gctcggctat cgcggcgatg agccggcgcc cgatggcgca aacctcgggc
      421 tcggttgcgg caatccgacg gtgatcgcat cgctgcggcc gggcgagcgc gtgctggacc
      481 tcgggtcggg cggcggcttc gactgcttca tcgccgcgca tcaggtcggc gagacgggca
      541 gggtcatcgg cgtcgacatg acggcggaca tggtctcgct cgcgcgagcg aatgcgcgca
      601 aggtgaacgc gacgaatgtc gagtttcggc tcggcgagat cgagcatctg ccggtcgcgg
      661 attcctcgat cgacgtcatc ctgtcgaact gcgtgatcaa cctgtcgccc gataagcggg
      721 ccgtgtttgc ggaggcgatg cgggtgctcg cgccgggtgg ccgtcttgcg atcgcggaca
      781 tcgtcgcgac gagggcgatc ccaacgcaga tgcagaacga cgtcgcagcg ctatgtggct
      841 gtgttgccgg tgcggcgttg ctcgacgacg ttcgcgcgat gctcgtcgat gtcggcttcg
      901 gcgacgtcga ggtgagcgtc acgccgcgca gcgcggagat tctcggcggc tggatgccgg
      961 gcatcgatca gtacgtcgcc tcggcgacca tcgaggctcg caaaccgggc gccatgcacg
     1021 cgcgcgcggg aggagcgacg agcgcaaccg ccggagaacc agcgaagcaa cgttcgtgtt
     1081 gcggaccgga gtgctgcgca tgaacgcgtc gcccgagcag ctgcgtagtg cctcgccggc
     1141 cggcgagctt tggcgcagcg tcgacgcgca gctgcgtccg ttcatcgcga agcgcgtcga
     1201 tgcggcggat gtcgacgatg tgcttcagga cgtcttcgtg cggatgcagc gcgggctcgg
     1261 tcaggtgcgc gaggaggaac gcttctccgg gtggctgttt caggtcgcgc gcaccgcgat
     1321 tgccgagcat ggacgcgcac gagctcgcca tccgctgccg aaggtcgatg tcgtcgagga
     1381 tgcgccggcc gggccctcga cggacgatga tcgcgtggcg tcacgagcgt tgtctggctg
     1441 cgtcgcggtg ttcgtcgcgc ggctggctac gccgtatcgc gaggcggtca cgctcgtcga
     1501 gctcgaagga ctcacggctc gcgaggcggc ggagatcgtc ggcatctccg tctcgggcat
     1561 gaagtcgcgc gtgcagcgag ggcggcagca gctgcgcgag atgatggaag cgtgctgcga
     1621 gatcgcgctc gatgcgcgtg gcaaggttac cgacttcgcg ccacgcgtcg accgctgcgc
     1681 ctgcggctct acttgaagct gtccttctcg ccgcgccgtg ctgcgaagtc cgccgcatct
     1741 gcagcgcgca agaacggatt ggtctcgcgc tcgaggccga tcagcgacgg cgtcgacggg
     1801 gtggcgagcg actcggcgcg ctgcttcatc gccgcgttgt caggctcggc gtgcatcgcg
     1861 tagcgaaggt tgctcgcggt gtattcgtgg ccgaagtaga ccttcgtctt cggcggcagc
     1921 gcggcgagct tctgcagcga cgcgtgcatc atctccatcg tgccttcgaa cacgcggcca
     1981 catccggcgc cgaacagcgt gtcgcccgtg aacacgcagt cgtcgatcaa gtagctgatc
     2041 gcgccgagcg tgtgccccgg gttgtggatg atcttcgtcc gcaatgggcc gcactggatc
     2101 gtctcgccgt cttcgacaac gtgcgtgacc ttgggtgcgc gtggcgcatc ggcggcgtgc
     2161 gcgacaacga cgagaccggg ggtttcttcg accagctttt cgacgccgcc gacgtggtcc
     2221 cagtggtggt gcgtcagcca gatcccgccg agagtgacgc cctctctctc gatggcagtg
     2281 cgaactggtg tgtgctcact cgggtccaca atgacggcgt aagttccttc gatgactaga
     2341 taagcgtagt tgtctttgag acacgggatt gccaggacgc gcatgtaaga aatgctacgc
     2401 cgatcggggt tcaggtgtgt ataacccggg cccatgcgaa tcctcctcgc ttcgttggta
     2461 gtggctttgg ttgcgtgcgg tccgtcgccg gacgaccgga cgatgggcga cgacgacatg
     2521 aacaccgact gcgaaggcat cgcgtgtcag gtcgtccagt gcgagaagat gggcaagccg
     2581 ccgacgacca tctcgggaac cgtctacgcg ccgaacggca cgctcgcgct ctacggtgcg
     2641 aacgtctacg tgccgctggt cgatccgggt ccgttcgcgg agggcgttac gtgcacgcag
     2701 tgccaagacc agctgccggg cggcgcggtc gcgaacggca tgagcgatac gtcgggcaag
     2761 ttcagcgtct cgggcgttcc gtcgggcacg gacatcccgc tgttcatcac gatcggcaag
     2821 tggcgccgca agacggtcat cccgcaggtg ctgccgtgcc aggacaaccc gcttccgaac
     2881 acggtcacca gcctgccgaa gaacaagacc gagggtgaca tcccgaagat cgcgatcgcg
     2941 accggcagct gcgacgcgct cgagtgcctc cttcgcaaga tcggtgttgc cgactcggag
     3001 ttcaccgcgg acagcggcag cggtcgcatc catctctact cggcgaacgg ctcgccgaag
     3061 atcgcgtcga gcaacacgat gctctcgccc gccacggcgc tgtggggcga cgtgaacaag
     3121 atgaagaact acgacatgat gctcttctcc tgcgagtgcg gtccgtaccc ggagaacaag
     3181 tcgcagccga tgatggacaa cctcaaggcg tatgccgaca tgggcggccg cgtgttcctc
     3241 tcgcactacc actcggtgtg gatctcgggt gagaagagcg ttccgacgca cgcaccggcc
     3301 gtgtggccga cggtcgcgtc gtgcaacatc gacgagtacc agagcggcac gggcgtgatc
     3361 gatcaggtca acaacccgaa gggctcggcg ttcgcgcagt ggatgacgaa cgtgcagggc
     3421 tcgacgacac cgggcacggt cgagatcaac gacagccgtc agtcgtgcac gtcggtcgac
     3481 aacacgaagg ccgagcgctg ggtctacatg aagatcggcg gcacggacta tccgcaggac
     3541 ttccagttca cgacgccgca agaagtcgcg aaggaagcgc gctgcggcaa ggtcgtgttc
     3601 tccgatatgc acgtcgcgtc gggttcgacg tcgagctcga ccggcttccc gtcgggctgt
     3661 ggtgccggcg cgatgtcgcc gcaagagaag gcgctcgcgt tcatgctgtt cgacatcgcg
     3721 acctgcgtcg gtccgatcct ctagctgcta caaacgcggt catggcgaac atgaccgcat
     3781 gcgtgctgcg cgagcacggc ggccccgagg tcttgcacct cgaagagctg ccactgccag
     3841 agcccgggcc cggtgaggtc cgcgttcgca ttcgcgccgt cgcgctgaac cacctcgata
     3901 tttgggtgcg gcgcggtggc cccgcgttca agctcgagta tccgcatcgc ctcggctccg
     3961 acatcgcggg tgaggtcgac gcgatcgggg cgggcgtgac cggcgtgacg gttggcgcga
     4021 agtgcgtcgt gcagccgggc atctcgtgca tgcgctgcac cgcgtgcctc ggtggtcacg
     4081 acaacctgtg tcgctactac aagatcctcg gcgagaacac gcagggcggc tacgcgcagg
     4141 tcatcgtcgt gccggcggtg aacatcgcgc cgtatccagg ggacctcgac tttccgaatg
     4201 ccgccgcatc ggtgctgccg tttctgacgg catggcagat ggtcgtgcac aaggcgcgcg
     4261 tgcagccggg cgagaccgtg ctcgtgcagg gcgccggctc gggcatcggt gtcgccgcga
     4321 tccagatcgc caagctgttc gctgcgcgcg tcatcacgac cgcgagcagc gaggacaagc
     4381 tcgcccgcgc tcgcgcgctc ggtgccgatg ccggcatcga ttactccacg caggacttcg
     4441 cgaccgag
//
LOCUS       DATLBZ010000472         3705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9989689, whole genome shotgun sequence.
ACCESSION   DATLBZ010000472 DATLBZ010000000
VERSION     DATLBZ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9989689"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VMZ53_23950"
     CDS             complement(<1..1283)
                     /locus_tag="VMZ53_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HUS31589.1"
                     /translation="MTLIRRAYQFAAQAHEGQTRKSGDPYVTHPLSVAQVIAELKLDV
                     ASVCAGLLHDCVEDTSATVEQLGEQFGKEIAFLVDGVTKLGKLPYSTREEQQAENFRK
                     MLLAMARDIRVILVKLCDRLDNMRSLNHLPPEKQERIAAETMQIYAPLANRLGIQWVK
                     VELEDLAFKYIYPGEYEQLAQEIAKTRAERLEYIHHVEKLILKEMQDNGVPCEVFGRA
                     KHLWSIYTKMKRTQRPFEEIHDSIGFRVITDTQMHCYQGLGVAHQTWTPIPGRFKDYI
                     ALPKPNLYQSLHTAVIGPRGERIEVQIRTKEMNLVAEHGIAAHWKYKEGKPLALEDDK
                     KFAWLRQLMESQKELRDPTEFLESVKIDLFGDEVYVFTPGGDVKALPKGSCPIDFAYA
                     VHSTIGDHCSGARVNGMIVPLRYQLRNGDTIEIIT"
     gene            complement(1398..2048)
                     /locus_tag="VMZ53_23955"
     CDS             complement(1398..2048)
                     /locus_tag="VMZ53_23955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31590.1"
                     /translation="MMGLRLLLVVVALVGCTRTFKAVAVQPNPLANQNETLRTSEKIT
                     IVRGDMELEMPDDPAPAQRASIVHNHRYPLLNEASFTMVSRDRLRFHVQVDHKWEEYA
                     DLNTWQVYLVDDKGRMWWPESVEHAHTHIITKMWDREQRTAVRNQYGDVVKINDDGFR
                     RRQTLGSLSVFRGNADFTFYQRDLFSVNVRSLKLVVKRSGEAFEFTWRFQDSVASQ"
     gene            complement(2118..2807)
                     /gene="gmk"
                     /locus_tag="VMZ53_23960"
     CDS             complement(2118..2807)
                     /gene="gmk"
                     /locus_tag="VMZ53_23960"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HUS31591.1"
                     /translation="MAGSQMNSRGVLVIVSSPSGAGKTTLTKRLLEELKGQLEFSVSY
                     TTRPMRTGEQHGKDYWFVSTDEFDEMVNKGEFAEWAFVHRNRYGTAAAPVETALAEGR
                     DVIFDVDWQGGKALSQRWPNDCLKIFVLPPDLETLERRLRQRATDAPHVVETRLRNAK
                     EELTHYREYQHLIVNDDLERAYALLRAMYLTRKYGEVARTDVPYDLPALANLVAANRV
                     AEAHAETLVGR"
     gene            complement(2807..>3705)
                     /locus_tag="VMZ53_23965"
     CDS             complement(2807..>3705)
                     /locus_tag="VMZ53_23965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HUS31592.1"
                     /translation="AGMRSMTGFGRGTQEHGRTRATVDIRAVNHRYLDLKLRGPLSPA
                     LEDAVGSRVRAAIDRGSVTVTVHLASASASSMRIDEAAAKGAHATLSGLAKTLGIEGP
                     TLALVLSQPGVVISADETESSEAEAPVLAAVDAAVAQLVSMREAEGKALAKELTARID
                     ELDRLRQQIATFAHDIPDRTRKKLQERLRRLLADEVTDKSSDAAGWLDPTRLAQEVAL
                     IAERADITEELVRLESHLGQARALITGKAAAGRRLEFLVQELGRELNTIGSKSAVTEI
                     STAIVEGKAVLEKVREQVQNVE"
BASE COUNT          609 a         1185 c         1186 g          725 t
ORIGIN      
        1 ctcgtgatga tctcgatcgt gtcgccgttg cgcagctggt agcgcagcgg gacgatcatg
       61 ccgttgacgc gcgcgcccga gcagtggtcg ccgatcgtcg agtgcaccgc gtaggcgaag
      121 tcgatcgggc agctgccctt cggcaacgcc ttgacgtcgc cgccgggtgt gaacacgtag
      181 acctcgtcgc cgaacaggtc gatcttgacg gactcgagga actccgtcgg atcgcgcagc
      241 tccttctgcg attccatgag ctggcgaagc cacgcgaact tcttgtcgtc ctcgagcgcg
      301 agcggcttgc cttccttgta cttccagtgg gcggcgatac cgtgctccgc gacgaggttc
      361 atctccttcg tgcggatctg aacctcgatg cgctcgccgc gcgggccgat cacggccgtg
      421 tgcagcgact gatagaggtt gggctttggc agcgcgatgt agtccttgaa ccggcccgga
      481 atcggcgtcc aggtctgatg cgcgacgccg agcccctgat agcagtgcat ctgcgtatcg
      541 gtgatcacgc ggaagccgat cgagtcgtgg atctcctcga acgggcgctg cgtgcgcttc
      601 atcttcgtgt agatcgacca caggtgcttc gcgcggccga acacctcgca cggcacgccg
      661 ttgtcctgca tctccttcag gatcagcttc tcgacgtggt ggatgtactc gagccgctcg
      721 gcgcgagtct tcgcgatctc ctgcgcgagt tgctcgtact cgccgggata gatgtacttg
      781 aacgcgaggt cttcgagctc gaccttgacc cactggatac cgaggcggtt cgcgagtggc
      841 gcgtagatct gcatcgtctc ggcggcgatg cgctcctgct tctcgggcgg caggtgattg
      901 agcgaccgca tgttgtcgag gcgatcgcag agcttgacga ggatgacgcg gatatcgcgc
      961 gccatcgcga gcagcatctt gcggaagttc tccgcctgct gttcctcgcg cgtcgagtac
     1021 ggaagcttgc cgagcttggt gacgccgtcg acgaggaatg cgatctcttt gccgaactgc
     1081 tcgccgagct gctcgacggt ggccgacgtg tcctcgacgc agtcatgcag aaggcccgca
     1141 cacacgctcg cgacatcgag cttgagctcg gcgatcactt gcgcgaccga gagcggatgc
     1201 gtgacgtacg gatcgccgga cttccgcgtc tgcccttcat gcgcttgcgc cgcgaactgg
     1261 taggcgcgac ggatgagcgt caagtcggcg ctggggtggt agcccgctac ttcactcaga
     1321 atctgatcga gctgatgcat cacaactgca ggtcccaaca gggtaccacg cagcgcagca
     1381 ttagctgact agctcaacta ctgggaagcg acgctgtcct ggaaacgcca cgtgaattcg
     1441 aacgcttcgc ccgatcgctt cacgacgagc ttgagcgacc gcacgttcac ggaaaacagg
     1501 tcgcgctgat agaacgtgaa gtccgcgttt ccgcgaaaca ccgagagcga gccgagcgtc
     1561 tggcggcggc ggaacccgtc gtcgttgatc ttgacgacgt cgccatactg attgcgaacg
     1621 gcggtgcgct gctcgcggtc ccacattttc gtaatgatgt gcgtgtgcgc gtgctcgacc
     1681 gactcgggcc accacatgcg gcctttgtca tcgaccaggt agacctgcca cgtgttgagg
     1741 tccgcgtact cttcccactt gtgatcgacc tgcacgtgga agcgcaggcg atcgcgcgac
     1801 accatcgtga agctcgcctc gttgagaagc gggtagcgat ggttgtggac gatcgatgcg
     1861 cgctgagcag gcgccgggtc gtccggcatc tcgagctcca tgtcgccgcg aacgatcgtg
     1921 atcttctccg acgtgcggag cgtctcgttc tgattagcca gcgggttggg ctgcacagcg
     1981 acggctttga acgtccgcgt gcatcccacc aacgcgacca caaccaagag cagtcggagg
     2041 cccatcatag gttcaggttg aatggtaccg cggtccgcgt caatgacgtg atcgggtgac
     2101 gagagctttg ctgagcccta ccgcccgacc agcgtttcgg catgcgcttc ggcgacacgg
     2161 ttcgcagcca cgaggttcgc gagggcggga aggtcgtagg gcacgtccgt ccgggctacc
     2221 tcgccgtact tccgggtgag gtacatcgcg cgtaacagcg cgtaagcccg ttcgagatcg
     2281 tcattcacga tgaggtgctg gtactcgcga tagtgagtca gctcctcctt ggcgttccgg
     2341 agccgggtct cgacgacgtg cggagcatcg gtcgcacgct gtcgcaggcg tcgctccagg
     2401 gtctcgagat cgggtgggag gacaaagatt tttagacagt cgttgggcca gcgctgagac
     2461 agcgccttcc cgccctgcca gtcgacatcg aagatcacgt cgcgaccttc cgcgagtgca
     2521 gtctcgacgg gagccgcggc ggtgccgtaa cggttgcgat gcacgaacgc ccactcggca
     2581 aactcgccct tgttcaccat ctcgtcgaac tcgtcggtcg agacaaacca gtagtctttg
     2641 ccgtgctgct caccggtgcg catcggccgc gtcgtgtacg agaccgagaa ctcgagctgg
     2701 cctttgagct cttcgagcag gcgcttcgtc agcgtggtct tgcccgcacc gctcggcgac
     2761 gacacgatga cgagaactcc gcgcgaattc atctgactgc ccgccatcat tccacgttct
     2821 ggacctgctc gcgcaccttc tcgagcacag ccttgccttc gacgatcgcg gtgctgatct
     2881 ccgtcaccgc actcttgctg ccgatcgtat tgagctcgcg gccaagctcc tgcacgagaa
     2941 actcgagtcg gcgtcccgcc gcggccttgc ccgtgatcag cgcgcgcgcc tgaccgaggt
     3001 gactctcgag ccgcacgagc tcttcggtga tgtctgcccg ctcggcgatc agcgcgactt
     3061 cctgcgcgag ccgcgtcggg tcgagccagc cggcagcgtc gctcgacttg tccgtgacct
     3121 cgtcggcgag cagacggcgc aggcgctctt gcagcttctt gcgcgtgcga tcgggaatgt
     3181 cgtgcgcgaa cgtcgcgatt tgctggcgca gccgatcgag ctcgtcgatg cgtgcggtca
     3241 gctccttcgc gagcgctttg ccctctgctt cgcgcatcga tacgagctga gcgactgccg
     3301 cgtcgaccgc cgcgagcacc ggtgcttccg cctcgctcga ctccgtttcg tcggcagaga
     3361 tcacgacgcc cggctgcgac aacacgagcg cgagcgtggg gccttcgatg ccgagcgtct
     3421 tcgcgaggcc ggagagagtc gcgtgtgcac ccttcgcggc cgcttcgtcg atccgcatcg
     3481 aggacgccga cgcgctcgcg agatgaactg tcacggtgac ggatccgcgg tcgatcgcgg
     3541 cgcgcacgcg actgcccacc gcgtcttcga gcgccggcga caatggaccg cggagcttca
     3601 gatcgagata gcgatgattg accgcgcgaa tgtcgacggt cgcgcgcgtg cgtccgtgct
     3661 cctgcgtgcc gcgtccgaag ccggtcatgc tgcgcatgcc agcaa
//
LOCUS       DATLBZ010000473        21548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1345336, whole genome shotgun sequence.
ACCESSION   DATLBZ010000473 DATLBZ010000000
VERSION     DATLBZ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21548
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345336"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..151
                     /locus_tag="VMZ53_23970"
     CDS             2..151
                     /locus_tag="VMZ53_23970"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS31593.1"
                     /translation="MLRALEQTGGIRKRAAVLLGISFRSLRYRLEKLGIEKPDSGDDE
                     DTGAK"
     gene            148..1044
                     /locus_tag="VMZ53_23975"
     CDS             148..1044
                     /locus_tag="VMZ53_23975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31594.1"
                     /translation="MRVWLWLLLALASFGIARGARVIAEAQQSTEAIGEPYAPTPGTA
                     PYVSLGYREAAADFLFVRLRTYFGDVDASPETVADLCEAIVVLDQRFHRPYEFCAGAI
                     SFFALTDRRHGSNALYRRAIALLDRGMAEYPNDWHLPNLAAQIYTQDLKTDDPAQRRE
                     WDEKATLLIESAVRKPGAPAELQEWAAVMRTKYGQQQRAIRDLHELLLVTSDKKAQRA
                     LIQRLAQLEHQDADAIAREVFEARHIFDEEWRNQRPSLPASWYVLLGPRLAPTFDPVD
                     LATGGHDLMQLQTPQPDEPPPE"
     gene            complement(1045..1782)
                     /locus_tag="VMZ53_23980"
     CDS             complement(1045..1782)
                     /locus_tag="VMZ53_23980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HUS31595.1"
                     /translation="MVEVLGVRIDPVTWIELRERVVHAIRERRPTTLMYANVHVLNTA
                     HEQPALADALATADVVYCDGEGVRVAARLAGSSLPERMTGADFIWDLARELATIRAKA
                     YWVGGAGDVGADALAILAERNPGLQVAGAHHGFFEKEGRESDAVIDAINASGADIVFV
                     GMGTPAQELWVARNRERIAAPVVWSIGATADFVAGVQPRGPAFLTQHGFEWLARLWSD
                     PRRLFGRYVIGNPLFVARVVRRRLARR"
     gene            1816..3207
                     /locus_tag="VMZ53_23985"
     CDS             1816..3207
                     /locus_tag="VMZ53_23985"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31596.1"
                     /translation="MRVAFITNYERTHVFHALSKHLEAAGHEVAYISPSTHWARWLEQ
                     QGVAPAHILDVATAGDEWDRDEVTPDEVAHLAELERRSKIYINDVILMDRILRHVPPN
                     RVRAYFAVATKRIARFLEDQDIQIVFGEQTFAFEILTTMVCASLGRLLLNPHVVRVPV
                     NRLGFYRFHQTSELVRFREVTPAHHAQAAELLAEFRRTKPIPDFIKRNQRPPMPRANW
                     PAKLLKHVRIELEDPHDETHFSPGWLIRQRSSEVLYGLAHRVMKPFWTPPEKPERPFV
                     LYPLQRQPESSIDVLGSRFSNQVELVRALARTVPSTHDLYVKEHPIGVGDRPPRVFRE
                     LRSIPGVKLVDPAVSSFALADAADLIVSVSGTAALESALRGRPAATIGKMFFGPILIA
                     SGFNPYADSVADLLAIREVASDDKLVAFLANVLAQSYVGTLGNPLHFPTALDADNIAS
                     VAIGFRDLLAAQQ"
     gene            3204..4433
                     /locus_tag="VMZ53_23990"
     CDS             3204..4433
                     /locus_tag="VMZ53_23990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31597.1"
                     /translation="MTGLLNVGLRGITMAARFVLLVVLAAYLAPAEVGVFALFTATNQ
                     WGIYLQGLELYLFTIREVVAAGPHEWARRTRDAFSLYGIVFAVASLLWTACFLGGFMP
                     WRLYAWFVVLLVLEQAGQELYRLLNAFGRPLAGSLVLFARTGSWMYVVSILMWRTPAY
                     RDLDTVFAAWVIGGSAAVVLALVFLRDLPWRGLPRVDWTWLRRGIAVSVPLLFGSLAW
                     RGIAVFERWYVGYAQGESALGVFGFYAAISAALPVLADSGIGAVLYPRLMKAWQANDH
                     TAYRHQLRQLWLSFGLLLAVCVPVAAIAMPFVVGHLAHGRYAAEVPTFNVLLAASALA
                     TLASVPQYALWSRGKDRSLVTIPIAGLATAVIADLILVPMYGGRGAAIGQLVAMALIL
                     VLRMGALVVADRREGAR"
     gene            4452..5858
                     /locus_tag="VMZ53_23995"
     CDS             4452..5858
                     /locus_tag="VMZ53_23995"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HUS31598.1"
                     /translation="MQAERNRPLRVFILILDALLVLAAMALAAGLHAALRDHVAALKD
                     PPRFDEYALLVYLTMPLWIGLEVLFGLHKTFERVWTRTQLALDLLKLHVAGFLGLSAA
                     VVATQATLNRSLILLFLGCSFLLSYAVRSALGLWQSYQHASGQARLRLLVIGEQSDQL
                     AAFLRTASAERRPPFVVGQLGVAPVGELPHLGRPDELERVLHENAVDHVLLFAPHHRG
                     DDVARIVSICETVGVRAELIVDLAEGAMSPPRVVNLYGRPFISFDSAAKKPEWIALKH
                     AFDWIVAAIGTVLLLPVLIVTALAILITMGRPILFTQTRAGLNGRPFRMFKFRTMVVD
                     AESRRQALADQNEMTGPVFKVTNDPRVTGLGRFLRKTSIDELPQLFNVLLGQMSLVGP
                     RPLPIDEQKAIRGWHRKRLSMKPGITGIWQISGRNNIDFEEWMRLDHEYIDAWSLRLD
                     LAILAKTVPAVMFRRGAR"
     gene            complement(5860..7581)
                     /locus_tag="VMZ53_24000"
     CDS             complement(5860..7581)
                     /locus_tag="VMZ53_24000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31599.1"
                     /translation="MSFVKRLRSPAVWFGCLTAAFAVTHIAALFTDAINWDEFALFSR
                     AQRAVLTGQLGTGGRPGLGVITLIPFIADCRDHLATVHAIRIAWSCFTFALLAGLFCF
                     LRRIARREPHAWHAAALGTALLALVPLFLRWSLQARTDQPAIAAALWAGVALLASRDR
                     RMFALASGVLLGTGYLFSEKATYIGALIVLVSLGDLYIDGEFRWRRELARSALFGTGV
                     LASIGIYKIVVPLIWVPQKQLSLDAGLDLFSWYRHVLGYRLYPSIVTSVIPHLVCAAI
                     IIICAVESFRKGGAERKPLALALAVMLLGYGIARFHTASFPYFWITIGVFPATAIAIG
                     WGSVRAVIPRFHTPLTIAAWVLLVALGVLYRRETLVDTQKTQRDTFALIEQRLPANWR
                     GFQTDAALVCRTDPSPIRVYLGQTIQTELFQGTFEAKRQALIDEFRSRPIAFIIRTQR
                     LNNFPDELREFWRDHYVPVASALEVAGKRVSGPRSTVDSIDVIVPGTYKWRAENRGAI
                     RIDDRDVAAGGTLELSTGRHDIALRDDVVGGVLALDVPTNVPVATAPFYPTMSLVELS
                     GVRRDWW"
     gene            7649..9604
                     /locus_tag="VMZ53_24005"
     CDS             7649..9604
                     /locus_tag="VMZ53_24005"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUS31600.1"
                     /translation="MQGARGTRFSAVLPTRWSWLALVASPSSYLLFIAIVVTMAAKAH
                     VVRGLEEISFWPGSWIAASAPDVAVFTGLAAVMSFVEARWRPARWLTPPIAFFVMLLA
                     ICSAAQLWITGEQLTAQVMVLGLERFGDVTAMARASVHVGPAAVIAIAALIAAPLVAH
                     RALRRSGQQDVGTRERARAAGILAVFAGLLGVVAPVPEQLAGLYRNAVAHTLWGLASG
                     DRSWNGGIGLFAGYEPHDLVDADTLAKLRAGPHPNVVVIVLESTRRDATGLDAAHSHA
                     RTPHLVELAARGLEMTHGRAVIPHTTKSLWSMMCGRLPILQPKLYETRPTGDVQCIPD
                     ILSAAGWRTGFLQSAVGKFEDRPRLVRGLGFQELVAAEQYTKEIGGYLSTDDEALVGQ
                     LAQWLDKKPGPFMVMMLTSSTHHPYMLTPSSEAYAKAESLPTSTDRDRYDRQIEAEDR
                     MVGGVLDLLRERGVLDHTIVVVLGDHGEGFGEKAARQHALNYYEEGLRVPWVITGPGI
                     SSQRVDVNTSVLDLAPTLLDLLGVQPTEAASASTPARSVLHLSTDRVLPFSCFFDKTC
                     FGFVQGSTKVIRMRETDRSFYFDLASDPEEHDPKQLTPALQTLLEEVAHTVDRHRTNS
                     KPVLREAMPDYPPWNCARNQPCGIKAP"
     gene            complement(9605..11392)
                     /locus_tag="VMZ53_24010"
     CDS             complement(9605..11392)
                     /locus_tag="VMZ53_24010"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HUS31601.1"
                     /translation="MATTWRQRLATLALERGGLLSLAILVLYLVLAPSFITDGDNAEF
                     STLAMTGGAGHPPGYPAYVLYLRAMSWLPAASGAHAAALATAIVGGATAFVLHQACRA
                     WGARPAACSIAVAIVSGAPVVLRLHTSAEVFAMNDLVAALVIWLAAAAGPLRGVRRAL
                     VLALVAGLGLADHHTCALLAPVGLLGVVRGIRESDNKAGATAAAFGGLLVGLSPYLYL
                     FGAPHSAASWGNPSSFSELLAHFTRKDYGGIGAFSPVPGEVDVGANLRALAMTLGRAW
                     LWAPAVIALGALVLRSVQRSEDGEPRSGWIALAITFLLAGPLLIARFNVPPVGIGLFI
                     CQRFHLLAVIVLTVPAAAALDAGFTLARRVEVGLLRRALVRELVAVAVFGAAAGASLP
                     HLLETHSPAVEKGVVNILQSLPPDAVVLGTADDLHFGALYVQQVRGIRKDVDVITWPM
                     TTMRWYREQFAARGLAIDPHAPGDEVPSLRVARQLLASGRPLFVEISMGTILQTFESY
                     PHGVLFRVLPPGTHKPDLDEILRINRDLFAAFDLAYPRPSHTSEYAAEMHQRYARTWD
                     ILARALAEAHRPDEARDARELVHELVPTP"
     gene            complement(11392..13050)
                     /locus_tag="VMZ53_24015"
     CDS             complement(11392..13050)
                     /locus_tag="VMZ53_24015"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HUS31602.1"
                     /translation="MATLGTIGGVAHPPGYPLYLIWLRAFSWLPAQSPAHASAIATAI
                     LGALGVLVLHAACRAWGARPRAASLTVAIFAAAPAVLRIQTEAEVFAMNNLIVALVLW
                     LSATRGPLRGYARIFALGLVAGFGLSDHLTCVLAAPVGILGVVRGVRETKTGAVGGIG
                     IAIAGLVLGLTPYLYLLATHDTNISWTRIDDVTHLVHHFLRKDYGGPGAFSPHGKEVS
                     ALESIWVMVQTVGRTWLWLPAIAGVAVLVRCIARAHDSETRVAWSMLAVSFVLAGPAL
                     ASRFNVVPEGIGLYVVRRFHILPALLLAPAVAVAFDHAATWLERRSEARAIRSDVVGG
                     VLAIISFAAFTGLSLDHVVRVHSPAVDLGLRQTLRSLPKDAVVIVHPDLMHFGLGYLQ
                     GAERERPDVTVISWAQVPSLTYRQRMAKSGIDLEPRANGRITTVDVAEQVLAKGRPLL
                     IDPYNSNIAQAFPTYPFGILFRVVPKEQRPPSIEEVFTTNRDLFEHFDFSYPMPGFDD
                     EYATDLHQQYARLWHTLAKGLEMAGRGEDAAVANAFAEALAPKP"
     gene            complement(13197..14984)
                     /locus_tag="VMZ53_24020"
     CDS             complement(13197..14984)
                     /locus_tag="VMZ53_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31603.1"
                     /translation="MSAFREYVKRDPGAWCLALAVVAVVVLYGATLGRGIVSYDDPWL
                     IRDNWVVHEASASSVRTILFDLHSNARLTLSPEYLPVRDLSVLLDFAIWGDWYGGFHL
                     TNLVVYVGAMCAWFGVLVGFGVDRRVAGLAILIWALHPAHAESVAWLSERKGLLAALF
                     TALCGLGYTRFRAGRSAGWLAFAALVGVMAVWSKATAAFGLAGLAGLDLVLPAGDRSR
                     RLVGLGVIGAFAVAAFVPVLMLATSANVVGTEMAAPAGRLEMVVGVLGFYVRLGLMTQ
                     HNSVSYPISDLGPSAIDVAVGIVALVALAWVLCAPRRMRLAPSPTLRAAGVLFVAGWL
                     PVSHLILPLQMVFVADRYLLFPTLGLALAIAYGVLRISSARARIALVAVLVFATALRT
                     LDAQSTWRDDSTLWQRAVETNPSDGNAWSMYADAVMDDGRTDLAFDVIHQGLRHTRAP
                     RLLLRKALLVQRMSTPEHAIVAMREAALAGEPRAMMNVGLLLLDVGHVDEALVWGMLA
                     AQLLPHHAPARRAFGKIALAAHHDELAYTEFTAAYRLEPKNRANRFNLALSLIALGRG
                     AEARPHLEVCLGDPELASRARGLLQQLAP"
     gene            15140..15640
                     /locus_tag="VMZ53_24025"
     CDS             15140..15640
                     /locus_tag="VMZ53_24025"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUS31604.1"
                     /translation="MIKRLKQRGFTLIELMIVVAIIGILAAVAIPAFMDYMKKSKKTE
                     ASLNLNKIAKNSKVYYNTNATFVNKAGAEAPGAGGANCSGPNKKFTVDATVWQDTAGW
                     QELDFQVDEPNLFSYTYTPGSTVATALAIGDLDCDKTLITYKLDLTAPGGNASAVITE
                     PPPNSD"
     gene            15713..17284
                     /locus_tag="VMZ53_24030"
     CDS             15713..17284
                     /locus_tag="VMZ53_24030"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31605.1"
                     /translation="MARRTKLALLAILCVYTALFVRAVTYELVWDDIHEIAESPDYDA
                     PLLDGALETQTARNDASLVETAKLQQDYDSFRPLTFASFWLEIRAWGRSPAPLHATNL
                     VLGALCTLLVFCLARRWVSDVAALCVTALFAIHPMQVEAVVYVSARGDLLAALGALLC
                     LAAVARANEASARSSIVGWTAAAGVAFAASVLAKESFACLPILVGVWLLLEGRLRGRW
                     PVAVVLAAIAIGYLVVRSAVVHNTAGSVVASAALALPGICVEYLRSFIAPFGLSTEQL
                     HASALTPIGIVLGAAALGGAAVAAWRRRSRELIAAVFGLCWVAILVAPSCVPIVSSSV
                     RADRYFYLPFAGLAVAIVAIVARLLDARPRIRRPAFAIAGIWLVVLLFISWRQVAVWR
                     TNETLYVNAVTMTPESSMANYRIGYLYATHDQWAEAAAYFESAIARDPDNALALNNLG
                     VMFMRQGDYRSAVAPLARATRANPGHFRAWLNLALSELQLGEHEQGCAHIQRALAINP
                     GYTTARAEWQQRCAR"
     gene            complement(17281..18261)
                     /locus_tag="VMZ53_24035"
     CDS             complement(17281..18261)
                     /locus_tag="VMZ53_24035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31606.1"
                     /translation="MEREQEVRTGTTQDTADVATETEEQSTPQTQFRSSGEIPAVASA
                     STAHAASASERAYAADTHHHGIPGGDRNVSMVGINHHPGHDSKGHGKQYTRVLVHGHE
                     PKLGKHARIFTSDGHAIGELEAGTHVPVNAGQICHLKIEGHEHPVSCVLAHGHPPGWV
                     PVSAFEHHGELAHIQHAQAHKIAHQQHNKPHAHGTPRTIRNKTIPADIDKLYTKPHQQ
                     SEGANHAHDYFIRGDGRANLLLNMPTWDTHGHSKGERLGSASDVVQAAEVGAEPNSHQ
                     VFHQTGSHVQVPLYNHGENHAVDHIRFVYGYVINSAGEHRYGWMNAFLLD"
     gene            complement(18273..21098)
                     /locus_tag="VMZ53_24040"
     CDS             complement(18273..21098)
                     /locus_tag="VMZ53_24040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31607.1"
                     /translation="MKKPTPFGKYYLLERINVGGMAEVFRAKAFGVEGFERLVAVKRI
                     LPNIAEDKEFIRMFIEEAKLAVQLNHANIAQIFDLGVVDGAYYIALEHVHGRDLRAMF
                     DRCRQIGEAMPVSQACFCVMKVCEGLDYAHNKRDQGGRELHLVHRDVSPQNVLVSFEG
                     EVKLIDFGIAKAAGKGSKTQAGILKGKFGYMSPEQVRGIPIDRRSDVFSCGIVLYELL
                     TGERLFVGESDFSTLEKVRNVEILPPSTYNRRIPDELERIVLKALAKDPDERYSNAID
                     LHDELQAFVYTAGEFYSRKDLAGWMKKTFGREIEEETAKLESYRQLKAPVAPVDEGRA
                     TNPVLGRTPTGSRPPTAAASPSARKSQPSGPQKTMPPPPPARPSGEFRAIQNTPGPQA
                     VTSPVKKQKRGPDDEGLAWDDDELETQIYDNPEDKAAAADVRRQAKQSGSHSANLPLS
                     SPPATPVPAPAAAAPPPPPPPPDLASLAGSTLKGWEKANGNGNGASPPAPMSIASGSN
                     KGPAPKPPVEDPLAALAASYSAAPSSSPFAAPPSAASARDLPMPKLTPPDPLFDPMAS
                     FGSGIVGRKRTSPSGSKNTLFIAIGGAAVAVVAVVVIIAMTGSKKDGPKSASAGTDST
                     QTTPTVNQAPTTAVDDPNTGFDLYVNPPGTMTWRLDGEERTTRLPSRVRGITPGVHTV
                     AIDAPAGYLSQNQSVTVEAGKAPRVDIMLQPIDISGTFSSDPAGATVSLIVDGKRETL
                     GPSPAKTKLDPRKTYQVLFEKTGYVSVNKPIVFTGSADEPISVVLEKAGAVAAVATQT
                     PKTETKTETQTQVKVQTPKTNTGGTPVTRPDTKTETKVETPKTETKTETKTEAKVETP
                     KVETPKAEAKGEGTLLLGSKPPCDIFIDGKATGLQTPQRDIKLTSGKHKITLVNNEFG
                     IKETFSVEIKPDAVEKQIKDYSDRIPK"
     gene            complement(21210..>21548)
                     /locus_tag="VMZ53_24045"
     CDS             complement(21210..>21548)
                     /locus_tag="VMZ53_24045"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS31608.1"
                     /translation="QVTDRRRELGLLRALGATKSDVLLVVLGEASVIGLIGGVLGVLL
                     GMGIASGIDYASAHYLPRFPYKPATWFNFKWWIWGGGLACSVGFAVLGGYLPARRAAT
                     MEPAQALSQN"
BASE COUNT         3581 a         7154 c         7080 g         3733 t
ORIGIN      
        1 ggtgctgcgc gcgctcgagc agaccggcgg gattcgcaag cgcgccgctg tgctcctcgg
       61 catcagcttc cgctcgctgc gctatcgcct cgagaagctc ggcatcgaga agcccgattc
      121 cggcgacgac gaggacaccg gcgcaaaatg agagtctggc tgtggctgct gctggctctc
      181 gcgagctttg gcatcgctcg cggcgcacgt gtgatcgcag aagcacagca gagcactgag
      241 gcgattggcg agccctacgc gcctacgccc ggtaccgcac cttatgtatc gctcgggtac
      301 cgcgaggcag ccgctgactt cttgttcgtc cgcttgcgca cgtacttcgg cgatgtcgat
      361 gcgtcgccgg agaccgtcgc ggatctgtgc gaggcgatcg tggtcctcga tcagcggttt
      421 caccgaccgt acgaattctg cgccggcgcg atctcgttct tcgcgctcac cgaccgccgg
      481 cacggcagca acgcgctgta ccgtcgagcg atcgccctgc tcgaccgcgg catggcggag
      541 tacccaaacg attggcacct cccgaacctg gcggcgcaga tctacacgca ggacttgaag
      601 accgacgacc ctgcgcagcg ccgcgaatgg gacgagaagg cgacgctgct catcgagtcg
      661 gcagtgcgca agcccggtgc ccccgcggag ctgcaggaat gggccgccgt catgcgcacg
      721 aagtacggcc agcagcagcg agcgattcgc gatctccacg agctcctgct ggtcacgtcc
      781 gataagaagg cgcagcgcgc gttgatccag cggctcgcgc agctcgagca ccaagacgca
      841 gacgcgatcg cgcgagaagt cttcgaagcg aggcacatct tcgacgagga gtggcggaac
      901 caacgcccgt cgcttcctgc gagctggtac gtactgctcg gcccgcgcct ggcgccgacg
      961 ttcgaccccg tcgatctcgc aacgggcggc catgacctca tgcagctgca gacgccccag
     1021 cccgacgagc cgccgccgga gtgatcagcg gcgcgccagc cgtcgcctga cgacgcgcgc
     1081 gacgaacagc gggttgccga tcacgtagcg gccgaacagc cggcgtggat cgctccacag
     1141 ccgagcgagc cactcgaacc cgtgctgcgt cagaaacgcc ggccctcgcg gctgcacgcc
     1201 cgcgacgaag tctgccgtcg caccgatcga ccacaccaca ggtgcagcga tgcgctcgcg
     1261 gttgcgagca acccacagct cctgcgccgg tgtgcccatg ccgacgaaga cgatgtctgc
     1321 gccgcttgca ttgatcgcgt cgatcaccgc gtcggactcg cggccttcct tctcgaagaa
     1381 gccgtgatgc gcacctgcca cctggagccc gggattgcgc tcggcgagga tcgcgagtgc
     1441 gtccgctccg acgtctcccg cgccgccgac ccagtaggcc tttgcgcgaa tggtcgcgag
     1501 ctctcgcgcg agatcccaga tgaagtccgc gcccgtcatg cgctcgggca acgagctgcc
     1561 tgcaagtcgc gccgcgacgc gaacaccctc gccatcgcag tagacaacat ccgctgtggc
     1621 tagcgcatcg gcgagagcag gctgctcgtg cgcagtgttg agcacgtgaa cgttcgcgta
     1681 catcagcgtc gtgggccgtc gctcacgaat cgcatgcacc acgcgctcgc gaagctcgat
     1741 ccacgtgacc ggatcgatcc ggacgccgag gacttcaacc attcgctgca tggtaccctg
     1801 gccacggtcc tggcgatgag ggtcgcattc ataacgaatt acgagcggac ccacgtcttt
     1861 cacgcgttat cgaagcacct cgaagcggcc ggccacgagg tcgcgtacat ctcgccgagc
     1921 acccactggg cgcgttggct cgagcaacaa ggcgtagcac ccgcacacat cctcgatgtc
     1981 gctaccgcgg gcgacgagtg ggaccgcgac gaggtgacgc cggacgaggt cgcgcatctc
     2041 gcagagctgg agcgtcgctc gaagatctac atcaacgacg tgatcctcat ggatcgcatc
     2101 ctgcgccacg tcccgccgaa tcgcgtgcgc gcctacttcg ccgtcgcgac gaagcgaatc
     2161 gcgcgcttcc tagaagatca ggacatccag atcgtcttcg gcgagcagac gttcgcgttc
     2221 gagatcctga cgacgatggt gtgcgcgagc ctcggccggc tcttgctgaa cccgcacgtc
     2281 gttcgcgtgc cggtcaaccg cctcggcttc tatcgctttc atcagacgtc cgagctcgtc
     2341 cgctttcgcg aggtcacgcc ggcgcatcac gctcaggccg ctgagctcct cgcagagttt
     2401 cggcgcacca aaccgattcc cgatttcatc aagcgcaacc agcggccacc gatgccacgc
     2461 gcgaactggc ctgcgaagct cttgaagcac gtccgcatcg agctcgagga tccgcacgac
     2521 gagactcact tctcgcccgg ctggctcatc cggcaacgca gcagcgaagt gctctacggc
     2581 ctcgcccacc gcgtgatgaa gccgttctgg acgccacccg agaagcccga acgtccgttc
     2641 gtcctgtacc cgctgcagcg tcagcccgag tcgtcgatcg acgtcctcgg cagccggttc
     2701 tcgaatcaag tggagctcgt tcgcgcgctc gcacgtaccg ttccatcgac gcatgatctc
     2761 tacgtcaaag agcatcccat cggtgtcggc gatcgaccgc cgcgcgtgtt tcgcgagctg
     2821 cgctcgattc caggcgtcaa gctcgtcgat ccagctgtgt cgtcgttcgc gctcgccgat
     2881 gccgccgacc tcatcgtcag cgtctcggga accgccgcgc tggagagcgc actccgtggc
     2941 cggccggcag caacgatcgg caagatgttc ttcgggccga ttctgatcgc gagcggcttc
     3001 aacccgtatg cggactcggt cgccgatctc ctcgccatcc gcgaggtcgc cagcgacgac
     3061 aagctcgtcg cgtttctcgc gaacgtcctc gcgcaatcgt acgtgggcac gctcggcaat
     3121 ccgctgcatt ttccgacggc gctcgacgct gacaacattg cgagcgtcgc cattggcttt
     3181 cgcgatctgc tcgcggcgca gcaatgaccg gacttctcaa tgtcggcctg cgcggcatca
     3241 cgatggcggc gcgcttcgtc ctgctcgtcg tgctcgcggc gtatctcgcg ccggcagagg
     3301 tcggcgtgtt cgcgctgttc acggcaacga accagtgggg catctacctc caagggctcg
     3361 agctgtacct cttcacgatt cgcgaggtcg tcgcggcggg tccacacgag tgggcgcgca
     3421 gaacacgcga cgcattctcg ctgtacggca tcgtgttcgc ggtcgcgtcg ctgctatgga
     3481 cggcgtgctt cctcggcggc ttcatgccgt ggcggttata cgcgtggttc gtcgtgctgc
     3541 tcgtcctcga gcaagcaggc caagagctct atcgcctcct caacgcgttc ggccggccac
     3601 tggcgggaag tctcgtgctg ttcgcgcgca ccggcagctg gatgtacgtc gtctcgatcc
     3661 tcatgtggcg cacgcccgcg tatcgcgatc tcgatacggt gttcgcggcg tgggtcatcg
     3721 gtggctctgc cgccgtggtg ctcgcgctcg tgtttctgcg cgacctgccc tggcgtggcc
     3781 tcccgcgcgt cgactggacg tggctgcgcc gagggatcgc ggtcagcgtg ccgctgctat
     3841 tcggcagcct tgcgtggcgc ggcatcgcgg tgttcgagcg ctggtatgtc ggctatgcgc
     3901 agggcgaatc ggccctcggc gtgtttgggt tctatgcggc tatctcagct gcgctgcccg
     3961 tgctcgccga ttctgggatc ggcgccgtgc tctatccgcg gctcatgaag gcgtggcagg
     4021 ccaatgacca caccgcatat cgtcaccagc ttcgccagct gtggctctcg ttcgggctcc
     4081 tccttgcggt gtgtgtgccc gttgcggcga tcgcgatgcc gtttgtcgtc ggccatctcg
     4141 cgcacggtcg ctacgccgcc gaggtcccga cgttcaatgt gttgcttgcc gcatcggcgc
     4201 tcgcgacgct ggcgtcggtc ccgcagtacg cgctgtggtc gcgcggcaag gatcggagtc
     4261 tcgtgacgat tccgatcgcg ggcctcgcga cagcggtcat cgccgatctc atcctcgtcc
     4321 cgatgtacgg cggtcgcggc gcggccattg gccagctcgt cgcaatggct ctcatcctcg
     4381 tgcttcgcat gggcgccctc gtggtcgcgg atcgccgcga aggtgcgcgt tgacgatgtt
     4441 aggctggtga tatgcaggcc gagcggaacc gtccgcttcg agtattcata ctgatactcg
     4501 acgctctgct cgtgctcgcg gcaatggccc tcgccgctgg tctgcatgct gcgctccgcg
     4561 atcacgtcgc ggcgctcaag gatccgccgc gcttcgacga gtacgcactg ctcgtgtacc
     4621 tgacgatgcc gctgtggatc ggcctcgaag tcttgttcgg actgcacaag acgttcgagc
     4681 gtgtgtggac gcgcacgcag ctcgcgctcg acctcttgaa gctccacgtc gcgggcttcc
     4741 tcggcctgtc ggcggctgtg gtcgcgacgc aggcgacgct caaccgcagt ctcatcctgc
     4801 tgttcctcgg ctgctcgttc ttgctcagct acgcggtgcg cagcgcgctc ggcctctggc
     4861 agagctatca acacgcgagt ggccaagcgc ggcttcgcct cctcgtcatc ggcgaacaaa
     4921 gcgatcagct cgccgcgttc cttcggacgg catcggcaga gcgacgcccg ccattcgtcg
     4981 tcggccagct cggtgtcgcg ccggtcggcg agcttcccca tctcggacgt cccgacgagc
     5041 tcgagcgtgt cctgcacgaa aacgcggtcg accacgtgct gctgttcgcg ccgcatcatc
     5101 gcggagatga cgtcgcgcgg atcgtgtcga tctgtgaaac ggttggcgtg cgtgcggagc
     5161 taatcgtcga tctcgccgag ggcgcgatgt cgccgccacg tgtcgtgaat ctgtatgggc
     5221 gcccgttcat ctcgttcgac tcggccgcga agaagcccga gtggattgcg ctgaagcacg
     5281 cgtttgattg gatcgtcgcg gcgatcggaa cggtgctgct gttgccggtc ctcatcgtga
     5341 cggcgctcgc gatcctcatc acgatggggc gcccgattct gttcacccag acgcgcgccg
     5401 gcctcaacgg tcgaccgttt cgcatgttca agtttcgaac gatggtggtc gatgccgaga
     5461 gccgtcgcca ggcgctcgcc gaccagaacg agatgacggg gcccgtcttc aaggtgacga
     5521 atgatccgcg cgtcaccggc ctcggcagat ttctgcgcaa gaccagcatc gacgagctac
     5581 cgcagctctt caacgtcctc ctcggacaga tgagtctcgt cgggcctcgc cctctgccga
     5641 tcgacgaaca gaaggcgatc cgcggctggc atcgcaagcg gctctcgatg aagcccggca
     5701 tcacgggcat ctggcagatc agcggccgca acaacatcga cttcgaggag tggatgcggc
     5761 tcgaccacga gtacatcgat gcgtggtccc tgcggctcga cctcgcgatc ctcgcaaaga
     5821 cagtccctgc cgtgatgttt cggcgcggcg cgcgctgagt caccaccagt cgcgtcgaac
     5881 gccgctgagc tcgacgagcg acatcgtcgg atagaacggc gcggtcgcga ccggcacgtt
     5941 ggtcggcaca tcgagcgcga ggactccgcc gacaacgtcg tcgcgaagcg cgatgtcatg
     6001 acgccccgtc gagagctcga gcgtcccgcc cgccgcgacg tcgcggtcgt cgatgcggat
     6061 ggctccgcgg ttctctgcgc gccacttgta cgtccccggg acgatgacgt cgatcgaatc
     6121 gaccgtcgac ctcggtccac tcacgcgctt gcccgcgact tccaacgccg aagcgacggg
     6181 cacgtagtga tcgcgccaga actcgcggag ctcgtccgga aagttgttga gccgctgcgt
     6241 tcgaatgatg aacgcgatcg gtcgcgagcg gaactcgtcg atgagtgcct gccgcttcgc
     6301 ttcgaacgtt ccttggaaga gctcggtctg gatcgtctgt cccagataca cgcgaatcgg
     6361 cgatggatcc gttcgacaca cgagagcggc gtcggtctga aagccacgcc agttggcggg
     6421 tagacgttgc tcgatgagcg caaacgtgtc gcgctgcgtt ttctgcgtgt cgacgagagt
     6481 ctcccgccga tacaagacgc caagcgccac gagcaggacc cacgcggcga tcgtcagcgg
     6541 cgtgtgaaaa cgcggaatga ccgctcgcac actgccccag ccgatcgcga tcgccgtcgc
     6601 ggggaacaca ccgatggtga tccagaagta cggaaatgac gcggtatgga agcgcgcgat
     6661 gccgtacccg aggagcatca ccgccagcgc gagagcgagc ggcttgcgtt cggcgccgcc
     6721 tttgcgaaac gactcgacgg cgcagatgat gatgatcgcc gcgcagacga gatgcgggat
     6781 cacgctcgtg acgatcgatg gatacagccg atagccgagc acgtggcggt accacgagaa
     6841 caagtcgaga cccgcgtcga ggctcagctg cttctgcgga acccagatga gcggcacgac
     6901 aatcttgtag atgccgatgc tcgcgagcac gccggtgcca aagagagcgg agcgcgcgag
     6961 ctcgcgtcgc cagcggaatt cgccgtcgat gtacagatcg ccgagcgaaa cgagcacgat
     7021 cagcgcgccg atgtacgtcg ccttttcact gaacaggtac cccgtcccga gcaagacacc
     7081 actggcgagc gcgaacatgc gccggtcacg agatgcgagc aacgcgacgc cggcccagag
     7141 ggcggcagcg atcgcaggct gatcggtcct cgcctgtaac gaccagcgca agaacagagg
     7201 cacgagcgcg agcagtgcgg tgcccaatgc ggccgcatgc cacgcgtggg gctcgcgccg
     7261 cgcgattcgc cgcaagaagc agaacaaccc cgcgagcagc gcaaacgtga agcacgacca
     7321 tgcgatccgg atcgcgtgca ccgtcgcgag gtggtctctg caatctgcaa tgaacggaat
     7381 gagcgtgatg acgccgaggc ccgggcggcc gccggtcccg agctgtcccg tgagcaccgc
     7441 acgttgtgcg cgcgagaaca gcgcgaactc gtcccagttg atcgcgtcgg tgaatagagc
     7501 cgcgatgtgc gtcaccgcaa atgccgcggt caggcatccg aaccagaccg ccggtgaacg
     7561 cagccgctta acgaagctca tcccacggcc ttccacccga gcatactcga tcgcgagcac
     7621 acgcctgcgc cctgctacca tgcggcgaat gcaaggtgcg cgaggcactc gatttagcgc
     7681 ggtgctaccg acgcgctggt cgtggctcgc gctcgtcgca agtccgtcga gctatctgct
     7741 cttcatcgcg atcgtggtca cgatggccgc gaaggcgcac gtcgtgcggg gcctcgagga
     7801 gatctcgttc tggccgggct cgtggatcgc ggcgagcgcg cccgacgtcg cggtgttcac
     7861 agggctcgcc gccgtcatgt cgttcgtcga agcgagatgg cgacctgcgc gctggctcac
     7921 gcccccgatc gcgttcttcg tcatgctgct cgcgatctgt agcgctgcgc agctgtggat
     7981 caccggcgaa cagctcaccg cgcaagtcat ggtgctcggc ctcgaacgct ttggcgacgt
     8041 gacggcgatg gcgcgcgcga gcgttcacgt cggccccgct gccgtcatcg cgatcgcagc
     8101 gctgatcgcc gccccacttg tcgctcatcg tgcgctgcgt cgatccggcc aacaggatgt
     8161 cggcacgcga gagcgagcac gcgccgcggg aatcctcgcg gtgtttgccg gtctgctcgg
     8221 cgtggtcgct cctgtgcccg agcagctcgc cggtctctat cgcaacgccg tcgcccatac
     8281 gctctgggga ctcgcgagcg gcgatcgcag ctggaacggc ggcatcggcc tgttcgccgg
     8341 gtacgagccg cacgacctcg tcgacgccga cacgctcgcg aagctgcgcg ccgggccgca
     8401 tccaaacgtc gtcgtgatcg tgctcgagtc cacgcgccgc gacgcgacgg ggctcgacgc
     8461 agcgcactcg cacgcgcgca caccacatct cgtcgagctc gccgcgcgcg ggctcgagat
     8521 gacgcacggt cgcgcggtca tcccgcacac gacgaagtcg ctgtggtcga tgatgtgcgg
     8581 gcgccttccg atcctgcagc ccaagctgta cgagacgcgg ccgacgggtg acgtccagtg
     8641 catcccggac atcctctccg cggccgggtg gcgcaccggc tttctccaga gcgccgtcgg
     8701 aaagttcgag gatcgccctc gcctcgttcg tggtctcggg tttcaagagc tcgtcgcggc
     8761 ggagcagtac acgaaagaaa tcggtggcta tctgtcgacc gatgacgaag cgctcgtcgg
     8821 gcagctcgca cagtggctcg acaagaagcc gggaccgttc atggtcatga tgctcacgtc
     8881 atcgactcat cacccgtaca tgctgacgcc gagctcggag gcgtacgcga aggcggagtc
     8941 gctgccgacg agcaccgatc gcgatcgcta cgatcggcag atcgaggccg aggatcgcat
     9001 ggtgggtggc gtgctcgact tgctgcgcga gcgcggcgtg ctcgaccaca cgatcgtcgt
     9061 cgtgctcggc gaccatggcg aaggattcgg cgagaaggct gctcgccagc acgcgctcaa
     9121 ctattacgaa gagggattgc gcgtgccgtg ggtgatcacc ggtccgggca ttagctctca
     9181 gcgcgtcgac gtcaacacga gcgtgctgga cctcgcaccg acgctcctcg acctgcttgg
     9241 cgtccagccc accgaggcgg cgagtgcatc gacgcctgcg cgcagcgtcc tgcatctttc
     9301 gaccgatcgc gtgttgccgt tctcgtgctt cttcgacaag acgtgctttg ggttcgtgca
     9361 aggcagcacc aaggtgatcc ggatgcgcga gaccgatcgc tcgttctact tcgacctcgc
     9421 aagcgatccc gaggagcacg atccgaagca gctgacgcct gcgctgcaga cgctgctcga
     9481 ggaagtagcc cacacggtcg atcgccatcg cacgaacagt aagccggtgc tccgcgaggc
     9541 gatgcctgac tacccgccgt ggaattgcgc acgcaatcag ccgtgcggga tcaaagctcc
     9601 gtagtcacgg cgtcggcacg agctcgtgga ccaactcgcg tgcgtcgcgc gcctcgtcgg
     9661 ggcgatgcgc ttcggcgagc gcgcgagcga ggatgtccca cgtgcgtgcg tagcgttggt
     9721 gcatctccgc cgcgtactcc gacgtgtggc tcggacgcgg atacgcgaga tcgaacgccg
     9781 caaacaaatc gcggttgatc cggaggatct cgtcgagatc cggcttgtgc gtgccgggcg
     9841 gcagcacgcg gaacagcacg ccatgcggat acgactcgaa cgtctgcagg attgtcccca
     9901 tcgaaatctc gacgaacagt ggccggccac tcgcgaggag ctggcgtgcg acgcgcaggc
     9961 tcggaacctc gtcgcccggt gcgtgcggat cgatcgccaa cccgcgcgct gcgaactgct
    10021 cgcgatacca gcgcatcgtc gtcatcggcc acgtgatcac gtcgacgtcc tttcggatgc
    10081 cccgaacctg ttggacgtag agcgcgccga aatgcagatc gtctgcggtg ccgagcacga
    10141 cggcatctgg cggcagcgac tgcaggatgt ttacgacgcc cttctcgacg gcgggcgaat
    10201 gcgtctcgag caagtgtggc agcgacgcgc ccgcggctgc gccgaacacg gcgacggcaa
    10261 cgagctcacg aacgagcgcg cgacggagaa gccctacctc gacgcgccgt gcgagcgtga
    10321 accctgcatc gagcgccgca gcggcgggga ccgtgagcac gatgacggcg agcaagtgga
    10381 agcgctggca gataaagagc ccgatgccga caggcgggac attgaagcgc gcgatgagca
    10441 gagggcccgc gagcaagaag gtgatcgcca gcgcgatcca gccgctgcga ggctcaccgt
    10501 cctcgctccg ttgcacgctg cgcagcacga gcgcgccgag cgcgatcaca gcgggggccc
    10561 acaaccacgc gcgtcccagc gtcatcgcca acgcgcgcaa gttcgcgccg acgtcgacct
    10621 cgcccggcac cggcgagaat gcgccaatgc cgccgtaatc cttccgcgtg aagtgtgcga
    10681 gcagctcgga gaacgagctc gggttgcccc acgacgcggc cgaatgcggc gcgccgaaca
    10741 gatacagata cgggctgagc ccgacgagca gcccgccgaa cgctgccgcg gtggcgcccg
    10801 ccttgttgtc tgactcgcga atcccgcgga cgacgccgag taagccgacc ggcgcgagca
    10861 gagcgcacgt gtgatggtcg gcgaggccga gtcccgcaac gagcgcgagc acgagcgccc
    10921 ggcgcacacc gcggagcgga ccggccgctg cagccagcca gatcacgagc gctgcgacga
    10981 ggtcgttcat cgcgaacacc tccgcggacg tgtgaagacg aagcaccacc ggcgcaccgc
    11041 tgacgatggc gaccgcgatc gaacacgcag ccggcctcgc accccacgcg cgacacgcct
    11101 ggtgcagcac gaacgcggtc gcgcctccga cgatggcggt ggcgagcgca gcggcatgtg
    11161 cacccgaggc cgcgggcagc cacgacatcg cgcgcagata caagacgtac gccggatacc
    11221 ccggcggatg gccggcgcca cccgtcatcg cgagcgtcga gaattctgcg ttgtcaccgt
    11281 cggtgatgaa tgacggcgcg aggacgagat acagcacgag gatcgcgagc gacagcagcc
    11341 caccgcgttc gagagcgagc gttgcgagcc gctggcgcca cgtcgttgcc atcatggctt
    11401 cggtgcgagc gcctcggcga acgcgtttgc cacggcggcg tcctcaccgc gtcctgccat
    11461 ctcgagtccc ttggcgagcg tatgccaaag ccgcgcgtac tgttgatgga ggtccgtcgc
    11521 atattcgtcg tcgaacccgg gcattgggta cgagaagtcg aagtgctcga acagatcgcg
    11581 gttcgtcgtg aacacctctt cgatcgacgg cggccgctgc tccttcggca cgacgcggaa
    11641 caagatgcca aacgggtacg tcgggaacgc ctgtgcgatg ttcgaattgt acggatcgat
    11701 caacagcggt cgtcccttcg cgaggacttg ttccgcaacg tcgacggtcg tgatgcgacc
    11761 gtttgcgcgt ggctcgagat cgatgccgct cttcgccatg cgctggcgat acgtgagcga
    11821 cggtacttgc gcccaggaga tgaccgtcac gtccggccgc tcgcgctcgg cgccctgcag
    11881 atacccgagg ccgaaatgca tgaggtcagg atgcacgatg acgaccgcat cctttggcag
    11941 cgaccgaaga gtctggcgga gcccgagatc gacggcgggc gaatgcacgc gcacgacgtg
    12001 gtcgagcgag aggcccgtga acgccgcaaa cgagatgatc gcgagcacgc cgccgacgac
    12061 gtcggatcgg atggcgcgcg cttcggatcg ccgctcgagc catgtcgctg cgtgatcgaa
    12121 cgcgaccgcc accgccggtg cgaggagtag cgccggcagg atgtgaaagc ggcggacgac
    12181 atagagaccg atgccctcgg ggacgacatt gaacctgctc gcgagcgccg gaccggcgag
    12241 cacgaacgag acggcgagca tgctccacgc aacgcgcgtc tcgctgtcat gcgcgcgggc
    12301 aatgcaccgc acgaggaccg ccacgccggc gattgcagga agccacagcc acgtgcgacc
    12361 gacggtctgc accatcaccc agatgctctc gagcgcgctg acttctttgc catgtggtga
    12421 gaacgcgccc ggcccgccgt agtccttgcg caggaagtga tgcacgaggt gtgtgacgtc
    12481 gtcgatacgc gtccacgaga tgttcgtgtc gtgcgtcgcg agcaagtaga gatacggtgt
    12541 cagaccgagg acgagacccg cgatcgcgat tccaatgccg ccgaccgcgc ctgtcttcgt
    12601 ctcgcgcacg ccgcgcacga ccccgagaat gccgacgggc gcagcgagca cgcacgtgag
    12661 gtgatccgac aggccaaagc ccgcgacgag cccgagcgcg aagatgcgcg catagccccg
    12721 aagcggcccg cgcgttgccg acagccagag gacgagcgcg acgatcaggt tgttcatcgc
    12781 gaacacttcg gcttcggtct gaatgcgaag gactgcagga gccgctgcga agatcgcgac
    12841 cgtcagcgac gcagctcgtg gccgcgcacc ccacgcgcga catgctgcgt gcaggaccaa
    12901 tacaccgagc gcgccgagga tcgctgtcgc aatcgcggac gcgtgcgcgg ggctctgtgc
    12961 ggggagccag ctaaaggctc gcagccagat caaatacagc gggtagccag gtggatgcgc
    13021 gacgccgccg atcgttccga gcgttgccat ctccgcgttg tcaccgtcga cgatcgttgt
    13081 cggcgcgagc cagatgtaca gcgcgagcgt cgcgagggcg attgcgcctc cgcgttcgaa
    13141 cgccagctga acgagccgct gtttcagagc gagtcatgat actcgcccct cgcagctcac
    13201 ggggcgagct gctgcaacaa gccacgcgcg cggctcgcga gctcgggatc gccgagacag
    13261 acctcgaggt gcggccgtgc ctccgcgccg cggccgagcg cgatcagcga gagcgcgagg
    13321 ttgaaccggt tcgcgcgatt cttgggctcg agccgatacg cggcggtgaa ctccgtatac
    13381 gcaagctcgt catgatgcgc ggcaagcgcg atcttgccga aagcgcggcg cgccggcgcg
    13441 tggtgcggca gcagctgcgc agcgagcatg ccccacacga gggcctcgtc gacgtggccc
    13501 acatcgagca gcaggaggcc gacgttcatc atcgcgcgcg gctcgcctgc gagcgctgcc
    13561 tctctcatcg cgacgatcgc atgctcgggt gtgctcatgc gctgcacgag gagtgccttg
    13621 cgcagcagga gccgcggcgc tcgtgtgtgt cgcaagcctt ggtggatcac gtcgaacgcc
    13681 agatctgtgc gcccgtcgtc catcaccgcg tcggcgtaca tgctccacgc atttccgtcg
    13741 gagggattcg tctcgacggc gcgctgccag agcgtactgt cgtcgcgcca cgttgactgt
    13801 gcatcgagcg ttcggagtgc ggtcgcgaac acgagcaccg caacaagcgc tatgcgcgca
    13861 cgcgcgctgc tgatccgaag tacgccgtac gcgatcgcga gcgcgaggcc gagcgtcggg
    13921 aacagcaagt agcggtcagc cacgaacacc atttgcagtg gcaggatcaa gtgactcacg
    13981 ggcagccaac ccgcgacgaa cagcacgcct gctgcgcgca gcgtcggcga gggcgcaagc
    14041 cgcatccgcc gcggcgcgca tagcacccac gcgagcgcga cgagcgcgac gattccaact
    14101 gcgacgtcga tcgccgatgg accgaggtca ctgatcggat agctcaccga gttgtgctgt
    14161 gtcatgaggc cgagccgcac gtagaagccc aggacgccga cgaccatctc gagtcgtcca
    14221 gccggagcgg ccatctcggt tccgacgacg ttcgccgacg tcgcgagcat cagcacggga
    14281 acgaacgccg ctactgcgaa ggcgccgatg acgccaagac cgacgagccg tcgcgagcga
    14341 tcgcccgccg gaagcacgag atcgaggccg gcgaggcccg cgagcccgaa cgccgcggtg
    14401 gccttgctcc acaccgccat cacgccgacg agcgccgcga acgcgagcca gcccgcagaa
    14461 cgccctgcgc gaaagcgcgt gtagccgagt ccgcacaacg cagtgaagag cgcggcaagc
    14521 agccccttgc gctcgctcag ccacgcgacc gactccgcgt gcgctggatg cagcgcccag
    14581 atgagaatcg cgaggccggc aacgcggcgg tcgacaccga acccgacgag gacaccgaac
    14641 cacgcgcaca tggcgcctac gtagacgacg agattcgtca ggtggaatcc gccgtaccag
    14701 tctccccaga tcgcgaagtc gaggagcacc gagagatcac ggaccgggag atattcgggc
    14761 gagagcgtca ggcgcgcgtt cgaatggaga tcgaacagga tcgttcgcac cgagctcgcc
    14821 gacgcttcgt gaacgaccca gttatcgcga atgagccacg ggtcgtcgta gctaacgatc
    14881 cctcgcccaa gcgtcgcgcc atacaggacg accaccgcaa cgaccgcaag cgcgaggcac
    14941 caagcacctg gatcgcgctt tacgtattcg cggaacgcgg acatggaact acatcgataa
    15001 cagccgagaa ttgacccagg agtgacgctt tacgtcacct ttcgatcgcc gcgttgccag
    15061 tcgtcatgga cttacggcca aaaagtcgtg gcacgtacgt tgctgctgta ctgtgctgcc
    15121 aaaagggggc atcagcgaaa tgattaagcg gctcaagcaa cgcggtttca cactcatcga
    15181 gctcatgatc gtcgtcgcga tcatcggcat cttggcggcc gtcgccattc cggccttcat
    15241 ggactacatg aagaagtcga agaagacgga agcatctctg aacctgaaca agatcgcgaa
    15301 gaactcgaag gtctactaca acacgaacgc gaccttcgtg aacaaggctg gcgcagaggc
    15361 tcccggcgcc ggtggcgcga actgctcggg tccgaacaag aagttcacgg tcgacgcgac
    15421 cgtctggcag gacactgccg gttggcagga gctcgacttc caggtcgacg agccgaacct
    15481 cttctcgtac acgtacaccc ccggctcgac ggtcgcgacc gcgctcgcca ttggcgacct
    15541 cgactgcgac aagacgctca tcacgtacaa gctcgacctg acggccccgg gtggtaacgc
    15601 ctcggctgtc atcacggagc cgccgccgaa ctctgactaa gaccaacggg tctttgagct
    15661 gaagcgccgc cgaaaggcgg cgttttcagt ttcggctcta tgatgagctc gcgtggcgcg
    15721 taggacgaag ctggcccttc tggcgatcct ctgcgtgtac accgcgctgt tcgttcgcgc
    15781 ggtcacgtac gagctcgtgt gggacgacat ccacgagatc gccgagagcc ccgactacga
    15841 tgcccctctg ctcgacggcg ctctcgagac gcagaccgcg cgcaatgacg cctcgctcgt
    15901 cgagaccgcg aagctccagc aggactacga cagctttcgg ccgctgacgt tcgcgagctt
    15961 ctggctggag attcgtgcat gggggcgctc gcctgcccca ctgcacgcga cgaacctcgt
    16021 cctcggcgcg ctgtgcacgc tgctcgtgtt ctgcctcgcc cgccgctggg tctcggatgt
    16081 ggccgcactc tgcgtcacgg cactgttcgc gatccatccg atgcaggtcg aggcggtcgt
    16141 ctacgtgtct gcacgcggtg acttgcttgc ggcgctcggc gctctcctct gtctcgctgc
    16201 tgtcgcgcgc gcgaacgaag catccgctcg ctcttcgatc gtcgggtgga ctgccgccgc
    16261 cggggtcgcg ttcgcggcat cggtgctcgc caaagagagc ttcgcgtgcc tgccgatcct
    16321 cgtcggcgtg tggctgctcc tcgaaggccg gcttcgtggt cgctggcccg tcgccgtggt
    16381 gctcgccgcg atcgcgatcg gctatctggt tgtccgctcc gccgtggtcc acaacaccgc
    16441 tggctccgtc gtcgcctccg cagcgctcgc cctgcccggc atctgcgtcg aatacctgcg
    16501 ctcgttcatc gcgccattcg ggctctcgac cgagcagctg cacgcatccg cgctaacgcc
    16561 gatcggtatc gtgctcggcg cagcagcgct cggcggcgcg gcagtcgcag cgtggcgtcg
    16621 tcgctcgcgc gagctgatcg cggccgtctt cggcctgtgt tgggtcgcga ttctcgtcgc
    16681 accgagctgc gtcccgatcg tctcgtcctc ggtacgtgcg gatcgctact tctatctgcc
    16741 gtttgcaggg cttgcggtag cgatcgtggc gatcgtcgct cgcctcctcg acgcgcgccc
    16801 ccgcatccgc cggcccgcat tcgccatcgc tggcatctgg ctcgtcgtcc tgctgttcat
    16861 cagctggcgg caagtcgccg tctggcgcac gaacgagacg ctgtacgtca acgcggtgac
    16921 gatgacgccc gagagctcga tggcgaacta ccgcatcggc tacctctacg cgacgcacga
    16981 ccagtgggcc gaagccgcgg cgtacttcga gagtgccatc gcgcgcgacc ccgacaatgc
    17041 actcgcgctc aacaacctcg gcgtgatgtt catgcgtcag ggcgactacc gcagcgccgt
    17101 cgcgccgctc gctcgcgcga cgcgtgcaaa ccccggccac ttccgcgcct ggttgaacct
    17161 cgcgttgtcc gagctgcagc tcggggagca cgagcaaggc tgcgcgcata tccaacgcgc
    17221 gctcgcgatc aatccgggct acacgacggc gcgcgccgag tggcagcagc gctgcgcgcg
    17281 ttagtcgagc aggaacgcgt tcatccagcc gtagcgatgc tcgcccgcgc tgttgatcac
    17341 gtagccgtac acgaagcgga tgtggtcgac ggcgtggttc tcgccgtggt tgtagagcgg
    17401 cacctgcacg tggctgccgg tctgatggaa gacctggtgg ctgttcggct cggcaccgac
    17461 ctccgctgcc tgcacgacgt cactcgccga cccgaggcgc tcgcccttgg agtggccgtg
    17521 cgtgtcccac gtcggcatgt tgagaaggag gtttgcgcga ccgtcgccgc ggatgaagta
    17581 gtcgtgcgcg tggttcgcgc cttcgctctg ttgatgtggc ttcgtgtaga gcttgtcgat
    17641 gtcggcggga atcgtcttgt tgcggatcgt tcgcggcgtg ccgtgagcgt gcggcttgtt
    17701 gtgctgctga tgcgcgatct tgtgggcctg tgcgtgctgg atgtgcgcga gctcgccgtg
    17761 gtgctcgaac gcgctcaccg ggacccagcc cggcgggtgg ccgtgcgcga gcacgcagct
    17821 gaccgggtgc tcgtggcctt cgatcttcag gtgacaaatc tggccggcgt tgactggcac
    17881 gtgggtgccc gcttcgagct cgccgatcgc gtggccgtcg ctcgtgaaga tgcgcgcgtg
    17941 cttgccgagc tttggctcgt gcccgtgaac gagcacacgc gtgtactgct tgccgtggcc
    18001 tttgctgtcg tggccaggat ggtggttgat gccgaccatg ctgacgttgc gatcgccacc
    18061 ggggatgccg tggtgatgcg tgtccgctgc gtacgctcgt tccgaggcgg atgcagcgtg
    18121 cgcggtcgag gcggacgcga ctgccggaat ctcgccgcta ctcctgaact gagtctgcgg
    18181 cgtcgattgc tcttcggttt cggtcgcgac gtccgcggtg tcttgcgtgg tgcccgtgcg
    18241 tacttcttgc tcgcgctcca tgttcgacaa gattacttcg gaatgcggtc cgaataatcc
    18301 ttgatttgct tctcgacggc gtccggcttg atctcgactg agaacgtttc tttgattccg
    18361 aactcgttgt tgacgagcgt gatcttgtgc ttgcccgacg tgagcttgat gtcgcgctgc
    18421 ggcgtctgca ggcccgtcgc cttgccgtcg atgaagatgt cacacggcgg cttgctgccg
    18481 aggagcagcg tgccctcgcc tttggcttcc gccttcggcg tttcgacttt cggagtctcg
    18541 accttcgctt cggtcttggt ctcggtcttc gtctcggtct tcggcgtctc gaccttggtt
    18601 tcggtcttcg tgtcgggacg cgtgaccggc gtgccaccgg tgttcgtctt cggcgtttgg
    18661 accttcacct gcgtctgcgt ctcggtcttc gtctcggtct tgggagtctg cgttgcgacc
    18721 gcggcgactg cgccggcctt ctcgaggacg acggagatgg gctcgtcggc ggagcctgtg
    18781 aacacgatcg gcttgttcac cgagacgtag ccagtcttct cgaacaggac ttgatacgtc
    18841 ttgcgcggat cgagcttcgt cttcgccggg ctcgggccga gcgtctcgcg ctttccgtcg
    18901 acgatcagcg agaccgtcgc gcccgcgggg tcgctcgaga acgtgccgct gatatcgatc
    18961 ggctgcagca tgatgtcgac gcgcggcgct ttgccggctt cgaccgtgac ggactggttc
    19021 tggctgaggt aaccggccgg cgcatcgatc gcgacggtgt ggacacctgg cgtgatgccg
    19081 cgaacgcgcg atggcaggcg cgtggtgcgc tcctcgccgt cgagacgcca cgtcatcgtg
    19141 cccggtgggt tcacgtagag atcgaagccg gtgttcgggt cgtcgacggc ggtggtcggc
    19201 gcttggttga ccgtcggcgt ggtctgcgtc gagtcggtac cggcagacgc gctctttggg
    19261 ccgtccttct tgctgcccgt catcgcgatg atgacgacga ctgcgacgac ggcaacggcg
    19321 gctccgccga tcgcgatgaa cagcgtgttc ttgctaccgg acggcgacgt gcgcttgcga
    19381 ccgacgatgc cgctgccaaa cgacgccatc ggatcgaaca gcggatccgg cggcgtgagc
    19441 ttcggcatcg gcaggtcgcg cgcggaggcg gctgacggcg gtgcggcgaa cggtgaggag
    19501 ctcggcgcgg cggagtagct cgcagcgagc gcggcgagcg gatcttcgac gggcggcttg
    19561 ggcgcggggc ccttgttcga gccactcgcg atcgacatcg gcgccggcgg cgacgctccg
    19621 ttgccgttgc cattcgcctt ctcccagccc ttgagcgtcg agcccgcaag cgatgcgaga
    19681 tcgggcggcg gcggtggtgg tggcggtgca gcagctgccg gcgcgggcac cggtgtggcc
    19741 ggcggcgagc tgagcggcag gttcgccgag tgcgagccgg actgcttcgc ctggcgacga
    19801 acgtcggcgg ccgcggcctt gtcctcgggg ttgtcgtaga tctgcgtctc gagctcgtcg
    19861 tcgtcccacg cgaggccttc gtcgtcgggc ccgcgcttct gctttttgac ggggctcgtg
    19921 accgcctgtg gtccgggcgt gttctggatc gcgcggaact cgccgctcgg acgcgccggt
    19981 ggcggcggcg gcatcgtctt ctgcggaccc gatggctgcg acttgcgcgc gctcggagac
    20041 gctgcggccg tcggcgggcg cgagcccgtc ggggtgcggc cgaggaccgg gttcgtcgca
    20101 cgaccttcat cgaccggggc gaccggcgcc ttgagctggc gatagctctc gagcttcgcg
    20161 gtctcttctt cgatctcgcg accgaacgtc ttcttcatcc aacccgcgag gtccttgcgc
    20221 gagtagaact cgcccgcggt gtagacgaac gcttgcagct cgtcgtggag atcgatcgcg
    20281 ttcgagtaac gctcgtccgg atccttcgcg agagccttga gcacgatgcg ctcgagctcg
    20341 tcggggatgc ggcggttgta cgtagacggc ggaagaatct cgacgttgcg gactttctcg
    20401 agcgtcgaga agtcggactc gccgacgaac agacgctcgc cggtgagcag ctcgtagagc
    20461 acgatgccgc acgagaacac gtcgctgcgg cgatcgatcg gaatgccacg gacttgctcc
    20521 ggcgacatgt agccgaactt gcccttgagg attcccgctt gggtcttgct gcctttgcct
    20581 gcggcctttg cgatgccgaa gtcgatcagc ttgacctcgc cctcgaacga aacgaggacg
    20641 ttttgcggcg agacatcgcg atgcacgagg tgcagctcgc ggccgccctg gtcgcgcttg
    20701 ttgtgcgcgt agtcgaggcc ctcgcagacc ttcatcacgc agaagcacgc ctggctgacc
    20761 ggcatcgcct cgccgatctg gcggcagcga tcgaacatcg cgcgcaggtc gcggccgtgc
    20821 acgtgctcga gcgcgatgta gtaggcgccg tcgacgacgc cgagatcgaa gatctgcgcg
    20881 atgttcgcgt ggttcagctg aaccgcgagc ttcgcctcct cgatgaacat gcggatgaac
    20941 tctttgtcct ccgcgatgtt tggcaggatg cgcttgaccg caacgagccg ctcgaaccct
    21001 tcgacgccaa atgctttggc gcggaacact tcggccatac cgccgacgtt gatccgctcg
    21061 agtaggtagt acttgccgaa aggcgtcggc ttcttcacgg tgcgtttatc ccgccgcccg
    21121 gggtgaaccg ggtcggctcc attgtcgggc cacaagagct ctctccctcg tgggccgagg
    21181 tgataccgcg aagactggca cgattacagc taattctgtg acagtgcctg cgccggttcc
    21241 atcgtcgcag cacgacgtgc cggtaggtag ccgccgagga cggcgaaacc gacggagcag
    21301 gcgagaccgc cgccccagat ccaccacttg aagttgaacc aagtcgcggg cttatacggg
    21361 aagcgcggca ggtagtgcgc cgatgcgtag tcgatgccgc tcgcgatccc catgccgagg
    21421 agcacgccga gcacgccgcc gatcaggccg atgacggacg cctcaccaag tacgacgagc
    21481 agcacgtcgc tcttggtggc gccgagcgca cgcaagaggc cgagctcccg gcgccgatcg
    21541 gtgacctg
//
LOCUS       DATLBZ010000474        19781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5817240, whole genome shotgun sequence.
ACCESSION   DATLBZ010000474 DATLBZ010000000
VERSION     DATLBZ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19781
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5817240"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2278
                     /locus_tag="VMZ53_24050"
     CDS             <1..2278
                     /locus_tag="VMZ53_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31609.1"
                     /translation="VAHVVDRPVDVVNEQLAAAVAARVIGAGRSTDEVVFSHVLIQEV
                     LYNELPTTQRASLHGSIADVLLARSSTQSFAEVAHHLFAAIPVRGIAAAIDTARRAAA
                     DASTHLAFDDAVALLERSLSEVPAAVEQDALRADLLLELARALFVAGRPSDGRNRALE
                     AVSVSRRLEDAERLARAVLLVGSAPRFATVDPELVALLEETLATLPAADSPLRARVLG
                     RLGAAKQPAPEFEPPIQLAREGLAMSRRTKADAATHLEVLHSATAAMGFFADPSERIA
                     LNQELATVASRLGDKPRTLRGHLRLVFDYIESGDPVQADACIEVCARLAAQLGRPAYQ
                     WSIPLVRAMRNVMQGEFAAADAHVEEARSIASKAEDSNHEASFTFFRYGLLRTAARNS
                     ELAEFMPIAAPMIRQLTDLHYSAACIAGGYARVGNLPQARATLDLLTSRLPELAGRQS
                     TVWVAEAAAALDDSALSESLIPLLAPIASRNLANGLWTMWCEGPVTRALGLVATTAHR
                     FDEAEKYFETALERVDVLGAPPHRARIEVDYAAMLRKRNQSGDRDRAMRLTDAAREKA
                     DRIGLPDLFASPQPSKTSDGFELRPDGDFWTITEAATVFRLKDSRGLRIVALLVASPD
                     REFHVLDLSSTPGEAAQAEDAGEALDARAIAAYKERVVELRQQLAQAEDWADRARATR
                     LREELEAVAGELAAGLGLGGKARRASSNVERARVNVRKRVLDAIMRIGEHSPSLAKHL
                     EQAIKTGTFCAYHPSRVR"
     gene            2367..2840
                     /locus_tag="VMZ53_24055"
     CDS             2367..2840
                     /locus_tag="VMZ53_24055"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUS31610.1"
                     /translation="MKKIVLSLALVVSLVAVASAETRKAPPVPAQNPTFVEIEKTLGF
                     VPEFFKGVPQTLLPAFWSSLTGLQLNPATKLDGKTKELIGLAVAAQIPCEYCVYFHTE
                     AAKLNGATDEEVKEAVAMAGVTRLASTILNGTQTDKAQFKKDTQRIIEGARRAKK"
     gene            2932..3405
                     /locus_tag="VMZ53_24060"
     CDS             2932..3405
                     /locus_tag="VMZ53_24060"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUS31611.1"
                     /translation="MKKIALSFALVASLVTVASAETRKAAPAPQNPTLGEIQKTFGFV
                     PDFVKGVPEALLPAFWGGITGLEMNPATKLDGKTKELIGLAVAAQVPCEYCVYFHTEA
                     AKLNGATDEEIKEAVAMASLTRMGSTILNGNQTDKAQFKKDIQRIVDGAKAHAKK"
     gene            3414..4451
                     /locus_tag="VMZ53_24065"
     CDS             3414..4451
                     /locus_tag="VMZ53_24065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS31612.1"
                     /translation="MTSFIDRILGDSAAFTQIAMAALGDRLGLWKDLAANGPATSTQL
                     ARRMGLSERHVREWLAAMAAASYLAYDTTTAQFALGSEHAAVLADEGGPMFLGGIPDL
                     LVSYMKPYDRLVETFRTGGGVPQSAYPEATYRGQERFSAGWYEHLLVQKWLPAAGLDG
                     ALASGLRLCDVGCGGGRAIVRLAKAYPASQFVGYDVYGPNVHRARELAERAGVAQRVQ
                     FHQLDAAAGIGQSFDVITTFDVVHDTAHPGQLLESIRGGLAARGTYLCLEIKCAARLE
                     DNFGPVGAMLHGVSVLYCMSTSLAHGGAALGTCGLHEGRLRELASMAGFRNVDKLAID
                     DAFNDLYALTA"
     gene            4494..7610
                     /locus_tag="VMZ53_24070"
     CDS             4494..7610
                     /locus_tag="VMZ53_24070"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS31613.1"
                     /translation="MGGPIVGRRAELHVLETALADARLGRGGIVLLVGEPGIGKSRLV
                     EEVARMAEGADVVWGRAWEAGGAPAYWPWTQVLRAVGGASTSPHVARVRGDSSEAAAS
                     SAGDRFFLFDAVARHLIAAATRPLVILFEDLHAADEPSLHLLAFLAAQLRAAAILVVG
                     TYRDVEARLTQGAGAVLDRIAREARVVRPARLGVDEVRELARNLGVIDSDALVTVYRR
                     SEGNPLFVVELLRVVQGRGVIGVPASVRTAIREHLAGLSGALRSVLEAVAVIGREVQP
                     VIVADICKRPLAEVQDELARGVEVGVLVERGGGRIAFAHGLIAETLHDDIPAARRHEL
                     HLAVANWLDRDPSPISSEIAHHLLEAGSDHADRAADAVRKAAERARRELAFEPAAQLI
                     ERCLGTAPTTNKLTRFELVRLLAEVRILGGDEAKGKESAREAAELARGLDSAELIARA
                     ALTHGLAYSFGVTDQVLVSLLEEALGALPPGDHPLRARMLARLGAAMTPAPVAAIPMQ
                     IARDAIAMARRLGDERTRLEVIYAALSALLPFAPPAERKPLSLEVLELAKRFDDPLVE
                     LRSLMRLTFDHFELADLVGVDCYIRAYDECLTRIRWSKAKWHVWMFRAMLAMLHGRFE
                     EQANAFNEAKRLAEEHGDGLWTLATRVGHELLTAWCRGDVETIERERLAFSAFASIPN
                     QTHYPEIGAAVHLWLGEHDAARSVMASHPVGRMVRDHHLPGAFTYTADLAWRLGDREL
                     AADLYPLLRDEEPPLPALHVHGFAFGRPMAHCAMILAATLGDLEASRRHFASCLSLVT
                     RIGARAFEAHACHAFSKITPDAAEAHDLAARARVIADEIGIRLDMREPTPPRVTATTS
                     PGIATSTAAATLRCEGEYWVVEWAAALCRVKATRGVEMLAKLVGEPGREFHALDLAGA
                     EVVDAGDSGEVLDAEAKRAYRARMLELREELDEAEQWNNAARAERARDEIEALESELS
                     RAAGLGGRDRRVGKAAERARVNVQRRLTDALKRISEINADLGKHFATTVRTGTYCSYS
                     PERATRPR"
     gene            7663..8640
                     /gene="msrP"
                     /locus_tag="VMZ53_24075"
     CDS             7663..8640
                     /gene="msrP"
                     /locus_tag="VMZ53_24075"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HUS31614.1"
                     /translation="MGALPMTDDIGESEITPRELYFNRRNFLRAGLMAGTVAGTAVLY
                     RKLNGVELDATATPEIRNVIPAPPQYKVAGESLTPRVSIVNYNNFYEFTTNKDGVAAA
                     SKNFKTDGWKISVEGMCHKPRIFDLADFLKLSPPEERVYRHRCVEAWSMVVPWVGFSV
                     SKLLAAVEPMGDAKYVAFETLLDPKRMPNQSNSSVLDWPYVEGLRMDEAMHPLAIFAT
                     GLYGQELPAQDGAPVKLVVPWKYGFKGIKSIVKITLTDKQPPTTWNKRAPKEYGFYAN
                     VNPGHPHPRWSQATEQRIGESGRRKTLMFNGYGEQVASLYAGMNLDVSF"
     gene            8627..10288
                     /locus_tag="VMZ53_24080"
     CDS             8627..10288
                     /locus_tag="VMZ53_24080"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HUS31615.1"
                     /translation="MSASKVVDGRFAKWLVIVNGLIPFGLLVYDAFGGQLGVNEVNFA
                     IRTTGLVGLVLLVLSLVVTPLRKLTGWSQLIAVRRNLGVLGFFYIALHFMIFFWWDRG
                     HDVGSTLSEIIERSYLWFGFGALVLMLPLAITSFDSMVSRLGAKRWKMLHRLTYPAIF
                     AGAIHYLLLVKSDTRQPKAFLYSLGALMLARVVMHHFDVRGQLAIAHKKLDAAKSAGS
                     KVKKFWKGELQIARIFQETPDVKTFRFVSADGGPLPFEHIAGQYINLHLTIDGKRVNR
                     SYTIASSPTRSAYCEISVKKAAFGSKWLHENWQEGQRVRVSAPAGKFFFAGHESERVV
                     LIAGGIGITPMMSVVRSLTDRGWRGEMYLLFSVKKDSDVVFKEELAYLQMRFPNLKVK
                     ITVGNRITKEMIASFVPGLKKGPVMLCGPDPMMTAMRKLLIEIGVPDAEVHQEAFIST
                     PPMEPGAPEPEVELPVGVAANIEFAKSGKTAESTELTVLEAAEECGVTIPYECRSGIC
                     GQCKTKLVSGKVAMEVQDALTPVDRARGLILACQARAVKDAVIDA"
     gene            complement(10289..11113)
                     /locus_tag="VMZ53_24085"
     CDS             complement(10289..11113)
                     /locus_tag="VMZ53_24085"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS31616.1"
                     /translation="MWLGRLAVLIALGGCGRHSFATVDGAAGGSDAPFIPAMPIAAYA
                     LNEGVGQNAASSVTTPMVNAVLGNSAGADGLDPTWTTGRFGGGLLFDATDDRATIAYA
                     PAGYFQTSSNNDGVSASAWIFPMGAGTTYRVVYEIGGESNGVSLGITATGILRCALRN
                     STAVQRSKVLDSTRTIATGVWSYVVCTSDAAGMNLFIYDNAGTAVDMTSASGPYIWRT
                     GNDGIAIGNNPADNSGLRGMITAGPEPFTGIVDEVRVYDRAISTAQIEYDLATALP"
     gene            complement(11142..11762)
                     /locus_tag="VMZ53_24090"
     CDS             complement(11142..11762)
                     /locus_tag="VMZ53_24090"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS31617.1"
                     /translation="MSPVAARRTQQQRREGTIRKLLDAATQTLIDVGYAEASVQRICE
                     RAGVSQGALFRHFATREELMVAVGADVGARILEDYKRKFASAVKRNTDIEAALLLVRA
                     ACRSRLNEAWYELEVAARTNENLRKALEPVLRHYFTNITALARELLPDVATALGPSFD
                     MLVTSIIMMFDGETTHRFLVRDHAVEEARLAYLVRGFELLQRQPHR"
     gene            complement(11759..13531)
                     /locus_tag="VMZ53_24095"
     CDS             complement(11759..13531)
                     /locus_tag="VMZ53_24095"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUS31618.1"
                     /translation="MAYDVIIKRGLYFDGTGAPGALKHLGIRDGRVAIASDEPLDERG
                     CSRVIDADGKWVMPGFVDCHTHYDAELIAAPSLSESVRHGVTTVTVGSCSISAVLAEA
                     EDCSDLFTRVESVPREHVLPLLKARKTWRSAAEYVEFLRRQALGPNVTSFLGHSDLRT
                     RVMGLERAVDPSVKPTASEMREMERLLEDALDAGFLGLSSMTNPWDKLDGDRFRSKQL
                     PSSYASWGEYRRLHKVLRRRGRILQSAPNIVTKINALLFMATSAGMFWRKSLRTTLIT
                     LADAKSSRGLHRVIGPMTRFVNRVLRGNLRWQTLPVPFEVYADGIDLVVFEEFGAGRD
                     ALHLASEVERNELMKDEAYRRRFRKDYERRGTARVWQRNFHDAFIVGCPDASLVGKSF
                     GEIADARGIHPVDAFLDLVVQHGKALRWRTTIANDRPAEMARLISERSALIGFADSGA
                     HIRNMAFYSFALRMLRYVRDTKSMPIERAVWRLTGEIGEWMGIEAGHLRVGDRADIAI
                     VDPAALDDRLDAYHEARMEGFGDLTRMVNRSDGAVAAVLINGRVAFDGTTFARDLGHA
                     ASYGTFLPARGTPPVMRTHVERAA"
     gene            complement(13618..16929)
                     /locus_tag="VMZ53_24100"
     CDS             complement(13618..16929)
                     /locus_tag="VMZ53_24100"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS31619.1"
                     /translation="MRVFGLCLLAGCALGATPPPSPPRRTDLGLAVSMSQLPNGLRVV
                     LVRDARAPEVSVTMRYAVGAIDDGAHPGIAHMAEHLMYQQVIANESLFTQLEEHATYF
                     NGGTTYDATTYLSRAPRAELDRLLSIEGLRLQLRCTTITDAIFERERAVVVNEIRERD
                     DSTQLLSAINASVFPDGHPYRQSIGGSEESLHALQRADVCAFIDAHYAPENAALVIAG
                     NLTPEEVEVALSKLVAKIPRRRVAPSGYVPALPTTAHRADATAPLDDEALLVTWPLPI
                     DPGVRTQVRAIAAALPYLINGEISGSVSSIELGDEGARVIGIVAFPASDESPWKVKEK
                     VESTIVELSDVFDPKWRLTELDQIGFDYVRQRAIYRLYRSLEDPPSRDQMLADFALAG
                     RDPSAALAAEFEGLRQLTQGTARDLVTRYLSIQGASFVTLKPETGKKRGHKVELAADI
                     HDVGQRRTTMDPAKARQPADVAVPEVAGITTKRLANGMQVVLLPLNSVPTVDVRLVFS
                     SGTADDPADKHGAAKLAAYGLGWNIRYINDLLAFAAAGGSNDIKVTTDHTTFSSAGID
                     MHIDYLLAGLRRWVRDGTYQRGTEGLVDAMRAAAKEEIDVDSTVADTWNAALYGSGHP
                     YTATGLRHMAATLGDADAERFRREHYTPDNATLVISGRFDAALTERWVEFLFGDWSGH
                     AVKKPLPRATLRPASIGLVEDIQQAQLRIALPTASTNRAAQLVVAQMLADIAADIRQQ
                     LGASYGVHAILGEERLATEYVLAGWVDQSRAPAAAELIAQRIAELHTDAGAAAQAFVI
                     ARRRVLVRLGALTASAEALGEWVEHDVDLGRAALSDVALAQQVRALTIDSLAGSLAEL
                     DLTRGVVFVRGPADSLKQAFAVLHRDPTLVQFDQAKRDSVADDPLARVEQAAAKSKPR
                     REQEDISYLEPALTEQAPRTRLTMKLMPFYIMGHLVESSEASLQIECCTGAGVAAELG
                     MRRDKAVALGLHVGVGYVSGYRYSGRGSTPNHYEVTDVPIDVAAFIQRTAYDRLWATL
                     LLGVHLDQLYKQSIDIYADDERGLHGALGIGLEGGLDLWKGHPHRLGIAARVQGALIS
                     SSGYGALSVGLAYRQ"
     gene            16952..17296
                     /locus_tag="VMZ53_24105"
     CDS             16952..17296
                     /locus_tag="VMZ53_24105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31620.1"
                     /translation="MRTFALVVLVLAACGKKSDSGAAASGGEVASCYSESMHSCREYR
                     GMNLALGSDSLTKLCTAVDKTAKFSDTPCATAGVIGTCKMDAGKDFFYDGYVLASSME
                     ADCKASSGTFAK"
     gene            complement(17297..18046)
                     /locus_tag="VMZ53_24110"
     CDS             complement(17297..18046)
                     /locus_tag="VMZ53_24110"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HUS31621.1"
                     /translation="MRLPCALLVTLAACAEAPTTIEPFLDLNKADGHPAHLEYLDRSR
                     LDMEEPSDLVRVGSKLFAVSDQHSKIYEITPDGDADEYLDIDGHDLEALGYDFTRNEF
                     LIADELKAKIWTIDEDGDRHDSIEVDNADDGNSGIEGIVVTTPDDRLFAVKEKDPARI
                     YELETDGTLVDSKKIDFAKDLSGISYNASDKHIYVLSDEDHTLFRLDRHWDPDRAWKL
                     PVDKPEGLAFSGNTLYIVSDSEDRIYTFELD"
     gene            18265..>19781
                     /locus_tag="VMZ53_24115"
     CDS             18265..>19781
                     /locus_tag="VMZ53_24115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31622.1"
                     /translation="MPGDAKDDSFGGSGAKEDGQYSACQLAEVLKLVNDSSSTASSLQ
                     DKVGLAENASRAIAAHRNGADGQQGTGDDDIYDDLNELDGVDFVGNLALGKLVYAVLP
                     KCENDLTGRPFIDDQTFTGPSSGWARDNAEVEVVLGVKGTTGQKLRELLLQKNSDGRT
                     LYDRMRKARVMEAFTYGFSLDEMPWDSDAQAAREKMPYVSLTIEPDRYAPNDQGEMEI
                     SLGTDLMDDTYYDTHGYTLLGNDIELRARARWDNATTVRRILIAAKFGTEIDADGNKV
                     NAKVDIRNDNGTSFVSKLDDDVRRGKTAWNGSDQAATPIRGVYEQLMAKNLLLDIGAH
                     HGVLLLEPAVHLRSTRSRYHMNEANITNVRAIYTNAVTRINAALAIVAKAKTAGIIPA
                     NQLAAVNAMETLGKGIVDKSLIAQRLGTTVTSLRLPDNWAQPSTPADLAANKKTAEAI
                     NALYHEFAAALDDTDRAITNAADEDFDAFTEMFRMWRISVESSFARKRSWDPFLNS"
BASE COUNT         3502 a         6297 c         6543 g         3439 t
ORIGIN      
        1 cgtcgcacac gtggtcgatc ggcctgttga cgtcgtcaac gagcagctcg ccgctgcggt
       61 cgcggcgcgc gtgatcggag cggggaggag cacggacgag gtcgtgttct cgcacgtgct
      121 gatccaggag gttctctaca acgagctgcc gacgacgcag cgcgcgtcgc tccatgggtc
      181 gatcgccgac gttctgctcg cacgctcgag cacccaatca ttcgcagagg tggcgcacca
      241 tctcttcgcg gcgattcccg tgcgcggaat tgcagcagcg atcgacacgg cgcgccgtgc
      301 agccgcggat gcaagcacgc acctcgcatt cgacgacgct gtcgcattgc tcgagcgctc
      361 gctctcggaa gtgccggccg ccgtcgagca agacgcgttg cgcgcggacc tgctgctcga
      421 gctcgctcgc gcgctgttcg tcgcaggccg cccgagtgat ggtcgcaatc gcgcgctcga
      481 ggcggtctcc gtcagccggc gtctcgagga tgcggagcga cttgctcgcg ccgtgcttct
      541 cgtcggctca gcgccacgat ttgcgacggt ggaccccgag ctcgtcgcgt tgctcgagga
      601 aacactggcg acgctgcctg cggccgattc gccgctgcgt gctcgtgtgc tcggccggct
      661 cggtgccgcg aaacagccgg cgccggagtt cgagccaccg atccagctcg cgcgcgaggg
      721 cctcgcgatg tctcgccgaa cgaaggccga tgccgccacg cacctggagg tgctgcactc
      781 ggcgaccgca gcgatgggat tcttcgccga tccgtccgag cgcatcgcgc tcaatcaaga
      841 gctcgccacc gtcgcgagcc gcctcggtga taagccacgc acgctgcgcg ggcatctgcg
      901 gctcgtcttc gactacatcg agagcggcga tccggtgcaa gcggacgcat gcatcgaggt
      961 gtgcgcgcgc ctcgcagcgc agctcggacg tccggcctat caatggtcga tcccgctcgt
     1021 tcgcgcgatg cgcaacgtga tgcaaggcga gtttgccgct gccgacgcac acgtcgagga
     1081 agctcgctcg atcgcatcga aggcggagga cagcaatcac gaggcgtcgt ttacgttctt
     1141 tcgctacggc cttctgcgca cggcagcgcg caacagcgag ctagcggagt ttatgccgat
     1201 cgcagcgccg atgatccggc agctgaccga tctgcattac tcagccgcgt gcattgccgg
     1261 cggttacgcg cgtgtcggta acttgccgca agcgcgtgcg actctcgatc tgctgacaag
     1321 ccggctgccc gagctggccg ggcgccagtc gacggtgtgg gtcgccgaag cggcagcagc
     1381 gctcgacgat tcggcgctct ccgagagcct catcccactg ctcgcgccga tcgcgtcgcg
     1441 caatctcgcg aatgggctct ggacgatgtg gtgcgaaggt cccgttacgc gcgcgcttgg
     1501 cctcgtcgcc acaacggcgc atcgattcga cgaagcggag aagtatttcg agaccgcgct
     1561 cgaacgggtg gacgtgctcg gtgctccacc gcatcgcgca cgcatcgaag tcgactacgc
     1621 ggcgatgctg cgcaagcgca accaaagcgg agatcgcgat cgcgcgatgc gcctcaccga
     1681 cgcagcgcgc gagaaagccg atcgcatcgg ccttcccgat ctgttcgcgt cgccgcagcc
     1741 ctcgaaaacg agtgacggct tcgagctgcg ccccgacggt gacttctgga cgatcaccga
     1801 agcggcgacg gtgttccgac tcaaggacag tcgtggtctg cgcatcgtgg cgctgctcgt
     1861 tgcctcgccg gatcgggagt tccacgtgct cgacctctcc tcgacacccg gcgaagcggc
     1921 gcaagcggaa gacgcaggcg aagcgctcga tgcacgcgcg atcgcggcgt acaaagaacg
     1981 cgttgtcgag cttcggcagc agctcgcgca agcggaagac tgggccgatc gcgctcgcgc
     2041 gacacgcctg cgcgaagagc tcgaagcagt cgccggagag ctcgcggcgg gcctgggtct
     2101 cggcggcaaa gcccgccgcg cgtcatcgaa tgtcgagcgt gcacgcgtca acgttcgcaa
     2161 acgcgtcctc gatgcgatca tgcgcatcgg cgagcacagc ccgtcgctcg cgaagcacct
     2221 ggagcaggcg atcaagaccg gcacgttctg cgcctaccat ccgagccgcg ttcgctaagc
     2281 ccgtggaacg caagcgagat tctcgccaca tttcgtgacg acgtttgcca cgccttggtt
     2341 gcacgaaccc caaccaagga actgacatga agaaaatcgt tctctcgctc gccctcgttg
     2401 tttcgctcgt agccgtcgcc tccgccgaga cccgcaaggc tccgccggtc cccgcgcaaa
     2461 atccgacgtt cgtcgaaatc gaaaagacgc tcggtttcgt gcccgagttc ttcaagggtg
     2521 tcccgcagac gctgctgccc gcattctggt cgagccttac cggcctacag ctcaacccgg
     2581 cgacgaagct agacggcaag acgaaggagc tcatcggtct cgccgtcgcc gcgcagattc
     2641 catgcgagta ctgcgtctac ttccacaccg aggcggcgaa gctcaacggc gcgaccgatg
     2701 aagaggtgaa ggaggccgtc gcgatggcgg gcgtcacccg tctggcgagc acgatcctga
     2761 acggcacgca gaccgacaag gcgcagttca agaaggacac ccagcgcatc atcgaaggtg
     2821 cgcggcgcgc gaagaagtag ctaaccaagc ggaacgaaag ggccgccgcg cagaacattt
     2881 cgttacggca tttggaacgc cttggtcttc gagacctaca aggaaatcca aatgaagaaa
     2941 atcgcactct cgttcgccct cgttgcttcg ctcgtgaccg tcgcttccgc cgagactcgc
     3001 aaggccgcac cggccccgca gaatcccacg ctcggcgaga tccaaaagac gttcggcttc
     3061 gtgcccgact tcgtgaaggg cgtccccgag gcgctgctgc ccgcattctg gggcggcatc
     3121 acgggcctcg agatgaatcc cgcgacgaag ctcgatggca agaccaagga gctcatcggt
     3181 ctcgcggtcg cggcgcaagt gccgtgcgag tactgcgtgt acttccacac cgaagccgcg
     3241 aagctcaacg gtgcgacgga cgaggagatc aaagaggcgg ttgcgatggc gagcctcacg
     3301 cgcatgggca gcacgatctt gaacggcaac cagacggaca aagcgcagtt caagaaggac
     3361 atccaacgca tcgtcgacgg cgccaaggcc cacgcgaaga agtgaggccc accatgacga
     3421 gcttcatcga ccgtatcctc ggcgactccg cagcgttcac gcagatcgcg atggcagcgc
     3481 tcggtgatcg actggggctg tggaaagatc tcgccgcgaa tggtcccgcg acgagcacgc
     3541 agctcgcgcg acgcatgggc ctctccgaga ggcacgtgcg cgagtggctc gcggcgatgg
     3601 cagcggccag ttacctcgcg tatgacacga cgaccgcgca gttcgcgctc ggctccgagc
     3661 atgcggcggt gctcgccgat gagggcggtc cgatgtttct cggcggcatc cccgatctgc
     3721 tcgtgagcta catgaagccg tacgaccgac tcgtcgaaac gtttcgcact ggcggaggcg
     3781 tgccgcagag cgcgtatccc gaggcgacat atcgcggaca agagcggttc tccgccggtt
     3841 ggtacgagca cttactcgtg cagaagtggt tgccggcagc aggtctcgac ggcgcgctcg
     3901 cgtcggggct gcgcctatgt gatgttgggt gtggcggcgg gcgcgcgatc gttcggctgg
     3961 caaaggcata cccggcatcg cagtttgtcg gctacgacgt ctacggtccg aacgtgcatc
     4021 gcgctcgcga gctcgcagaa cgcgcaggtg ttgcgcagag ggtgcagttt caccagctcg
     4081 atgctgccgc cggcatcggt cagagcttcg acgtgatcac tacattcgat gtcgtgcacg
     4141 ataccgctca tcccggacag ctgctcgagt cgattcgcgg aggtctcgca gcgcgaggca
     4201 cgtatctgtg tctcgagatc aagtgcgcag cgaggctcga ggacaacttc ggtcccgttg
     4261 gcgcgatgct gcatggcgtg agcgtcctct actgcatgtc gacgtcactc gcgcacggtg
     4321 gcgcagcact cggcacctgt ggtttacacg aaggccgctt gcgcgagctc gcgtcgatgg
     4381 cggggtttcg caacgtcgac aagctggcga tcgatgacgc attcaacgat ctctacgcgc
     4441 tgaccgcgta gccctagaca tcccgacggg acgcgatgga gactgatcga atcgtgggag
     4501 gaccgatcgt cggaaggagg gcagagctcc acgtgctcga gactgcgctc gccgatgccc
     4561 gattggggcg cggcggcatc gtgctcctcg tcggcgagcc cggcattggg aagagcaggc
     4621 tcgtcgagga agtcgcgcgg atggccgaag gcgccgacgt cgtgtgggga cgggcgtggg
     4681 aagctggtgg tgcgcctgcg tattggccgt ggacgcaagt gttgcgtgca gtcgggggtg
     4741 catcgacgtc gccgcatgtc gcgcgcgtac gaggtgactc gagcgaggcg gctgcatcga
     4801 gcgccggtga tcgcttcttc ttgttcgacg cagtagcacg ccatctgatc gctgcagcga
     4861 cgcgaccgct cgtgatcttg ttcgaggacc tgcacgctgc tgacgagcca tctttgcact
     4921 tgctcgcgtt tctagcagcg cagctgcgcg cggcggcgat cctcgtcgtg ggtacgtatc
     4981 gcgatgtcga ggcgaggctg acgcaaggcg ccggtgctgt cctggatcgc atcgcacgcg
     5041 aagctcgcgt cgtacgccct gcgcgccttg gtgtcgatga ggtgcgcgag ctcgcacgca
     5101 atctcggcgt gatcgacagt gacgcgctcg tgacggtgta ccggcgaagc gagggcaatc
     5161 ctctcttcgt cgtcgagttg ctgcgtgttg tccaagggcg cggcgtgatc ggcgttccag
     5221 cgagcgtacg cacggcgatc cgagagcacc ttgcggggtt gtcaggggcg ttgcggagcg
     5281 tactagaagc ggtcgcggtg attggccgtg aggttcagcc ggtcatcgtc gcagatattt
     5341 gcaaacgtcc gctcgccgag gtgcaggacg agctcgcgcg cggtgtcgag gtcggcgtgc
     5401 tcgtcgaacg cggcgggggc cgcattgcat tcgcacacgg gctaatcgcc gaaacgctac
     5461 acgacgatat tccggctgca cgccgtcacg agctgcacct ggcagtcgcc aattggctcg
     5521 atcgcgatcc gtcgccgata tcgagcgaga tcgcacatca cctgctcgaa gccggcagcg
     5581 accacgccga ccgggccgcc gatgctgtgc gcaaggcagc ggagagagcg cgtcgcgagc
     5641 tcgcgttcga accggcggcg cagctgatcg agcgatgcct cgggacggcg cctacgacga
     5701 acaagctgac gcggttcgag ctcgtacgct tgctcgcgga ggtacgaatc ctcggcgggg
     5761 acgaggcaaa gggcaaagag agtgcgcgtg aggcagcgga gctcgcgcgt ggtctggatt
     5821 cggcggagct catcgcgcgc gccgcgctga ctcatggtct cgcgtattcg tttggcgtca
     5881 ccgatcaggt actcgtgtca cttctcgagg aagcgctcgg cgcgctgccc ccgggtgatc
     5941 atccgctgcg cgcgcgcatg ctcgctcgtc tcggggccgc gatgacgcct gcacccgtag
     6001 cggcgattcc aatgcagatc gcgcgtgatg cgatcgcgat ggcgcgtcgg ctcggagatg
     6061 aacgtactcg tctcgaagtc atctatgcgg cgctgagcgc cctgttgccg ttcgcgccgc
     6121 ccgcagagcg caagcctctc agtctcgagg tgctagagct cgcgaagcgg ttcgacgacc
     6181 cgttggtcga gcttcgctct ctcatgcgcc tcacgttcga ccacttcgag ctcgccgatc
     6241 tcgtcggtgt cgattgctac atccgcgcgt acgacgaatg cctgacccgc atccgctggt
     6301 cgaaggcgaa gtggcacgtg tggatgttcc gcgcgatgct cgccatgctc catggccgct
     6361 tcgaggagca agcgaacgca ttcaacgaag ccaagcgcct cgctgaagaa cacggcgacg
     6421 gcctgtggac gctcgctacg cgcgtaggtc atgagctgtt gaccgcgtgg tgtcgcggcg
     6481 acgtcgagac gatcgaacgc gagcgcctcg cattctccgc gttcgcgagc atccccaatc
     6541 agacgcatta ccccgagatt ggggccgccg tgcatctctg gcttggcgag cacgatgcag
     6601 cgcgcagcgt catggcgagc catccggtgg gccgaatggt gcgcgatcat catcttcccg
     6661 gtgcgttcac gtacaccgcc gatcttgcgt ggcgactcgg ggatcgagag ctcgcggcgg
     6721 atctctatcc gctactgcgc gacgaggaac cgcccctgcc ggcgcttcac gtgcacgggt
     6781 tcgcgttcgg caggcccatg gcacattgcg cgatgatcct cgcggctacg ctgggcgacc
     6841 tcgaagcgtc gcgacgtcac ttcgcgagct gtttgtcgtt ggtcactcga atcggcgcgc
     6901 gagcgtttga agcccacgcc tgtcacgcat tctcgaagat cacgcccgat gcagccgagg
     6961 cacacgacct cgccgcgcgt gcgcgcgtga ttgcagacga gatcggcatc cggctggaca
     7021 tgcgggagcc aacgccaccg cgggtaaccg cgacgacttc gcctggcatc gcaacgtcga
     7081 cggcggctgc cacgttgcgg tgcgaaggtg agtattgggt cgtcgagtgg gccgccgcgc
     7141 tctgccgagt caaggcgacg cgtggtgtgg agatgctcgc aaagctcgtc ggtgagccag
     7201 gccgtgaatt tcacgcgctc gatctcgcgg gtgcagaggt cgtcgatgca ggtgacagcg
     7261 gcgaagtgct cgacgccgag gcgaagcgtg cgtatcgcgc tcgcatgctc gagctgcgcg
     7321 aagagctcga cgaggccgag cagtggaaca acgctgctcg cgccgaacgt gcccgcgacg
     7381 agatcgaggc cctcgagtcc gagctctcgc gggcggccgg cctcggtggt cgggaccgcc
     7441 gcgtcggcaa agccgcagaa cgcgcacgcg tcaacgtgca gcggcggcta accgacgcgc
     7501 tcaagagaat ctcggagatt aacgcggacc ttggtaagca cttcgccacc accgtccgca
     7561 cgggaaccta ctgttcctac tccccggagc gcgccacgcg cccgcggtaa cgggtcctgt
     7621 aaccactgcg cccgtcgggc gtatgtcacg atgattgatt agttgggggc gctgcctatg
     7681 accgacgata tcggggagag cgagatcacg cctcgcgagc tgtacttcaa ccgccgcaac
     7741 ttcctgcgcg cggggctcat ggcgggcacg gtcgcgggga cggcggtcct gtatcgcaag
     7801 ctcaatggtg tcgagctcga tgcgacggcg acgccggaga ttcgcaacgt gattcctgcg
     7861 ccgccgcagt acaaggtggc cggcgagtcg ctgacgccgc gcgtctcgat cgtcaactac
     7921 aacaactttt acgagttcac gacgaacaaa gacggcgtcg ccgccgcgtc gaagaacttc
     7981 aagaccgacg gctggaagat cagcgtcgag ggtatgtgcc acaagccgcg catcttcgac
     8041 ctcgcggact tcctcaagct cagcccgccc gaagagcgcg tctatcgcca tcgctgcgtc
     8101 gaagcgtggt cgatggtcgt cccgtgggtc ggcttctcgg tgagcaagct gctcgccgcg
     8161 gtcgagccga tgggagacgc gaagtacgtc gcgttcgaga cgctgcttga tccgaagcga
     8221 atgccgaatc agagcaacag ctcggtgctc gattggccgt acgtcgaggg cctgcgcatg
     8281 gacgaagcga tgcatccgct cgcgatcttt gcgacggggc tctacgggca ggagctacct
     8341 gcgcaggatg gcgcgccggt gaagctcgtc gtgccgtgga agtacggctt caagggcatc
     8401 aagtcgatcg tgaagatcac gctgaccgat aagcagccgc cgacgacgtg gaacaagcgc
     8461 gcgccgaagg agtacggctt ctacgcgaac gtcaatcccg gtcacccgca tccgcgctgg
     8521 agccaagcga cggagcagcg catcggtgag tcggggcgac gcaagacgct catgttcaat
     8581 ggctacggcg aacaggtggc gagcctctac gcggggatga atctcgatgt cagcttctaa
     8641 ggtcgtcgac ggccggttcg cgaagtggct cgtgatcgtc aacgggctca tcccgttcgg
     8701 gctcctcgtg tacgacgcgt ttgggggaca gctgggcgtc aacgaggtga acttcgcgat
     8761 ccgcacgacg ggcctcgtcg gtctcgtgct gctcgtgctg tcgctcgtcg tcacgccgct
     8821 gcgcaagctg accgggtggt cgcagctgat cgcggtgcgc cgcaacctcg gcgtgctcgg
     8881 gttcttctac atcgcgctcc acttcatgat cttcttctgg tgggaccgcg gccacgacgt
     8941 cggtagcacg ctctcggaga tcatcgaacg cagctatctg tggtttggct tcggtgcgct
     9001 cgtgctcatg ctgccgctcg cgatcacgtc gttcgacagc atggtgagtc gcctcggcgc
     9061 gaagcgctgg aagatgctgc accggctgac atacccggcg atcttcgcgg gcgcgattca
     9121 ctacctgctg ctcgtgaagt cggacacgcg ccaaccgaag gcgtttctct actcgctggg
     9181 cgcgctgatg ctcgcgcgtg tggtgatgca tcacttcgac gtgcgcggtc agctcgcgat
     9241 tgcgcacaag aagctcgacg cggcgaagtc agcgggctcg aaggtgaaga agttctggaa
     9301 gggcgagctg cagatcgcgc gcatcttcca agagacgccc gacgtgaaga cgttccgctt
     9361 cgtgtcggcg gacggtgggc cgctgccgtt cgagcacatc gcgggtcagt acatcaactt
     9421 gcatctcacg atcgacggca agcgcgtcaa ccgctcgtac acgatcgctt cgtcaccgac
     9481 gcgcagtgcg tactgcgaaa tctcggtgaa gaaggccgcg ttcggctcga agtggctgca
     9541 cgagaattgg caggaagggc agcgcgtgag ggtgtcggca ccggccggca agttcttctt
     9601 cgccggtcac gagtccgagc gcgtcgtcct gatcgcaggc ggcatcggca tcacgccgat
     9661 gatgtcggtc gtgcgcagcc tcaccgatcg tggctggcgc ggcgagatgt atctgctgtt
     9721 cagcgtgaag aaggacagcg atgtcgtgtt caaggaggag ctcgcgtatc tgcagatgcg
     9781 gttcccgaac ctgaaggtga agatcaccgt cgggaatcgg atcacaaagg agatgatcgc
     9841 gagcttcgtg cccggtctga agaagggccc ggtgatgctg tgcggccccg atccgatgat
     9901 gacggcgatg cgcaagctcc tcatcgagat cggcgtgccc gatgccgagg ttcatcaaga
     9961 ggcgttcatc tcgacgccac cgatggaacc gggcgcgccc gagcccgagg tcgagcttcc
    10021 cgtcggtgtc gctgcgaata tcgagttcgc caagagcggc aagacggcgg agtcgacgga
    10081 gctcaccgtg ctcgaagcgg ctgaggagtg cggcgtgacg attccgtacg agtgcaggtc
    10141 cggcatctgc ggccaatgca agacgaagct cgtctccggc aaggtcgcga tggaagtgca
    10201 agacgcgctg acgcccgtcg atcgcgcacg aggcttgatc ctcgcgtgcc aggcgcgcgc
    10261 agtgaaagac gccgtcatcg acgcgtagct acggtagggc agtcgcgagg tcgtactcga
    10321 tctgcgctgt cgagatggca cgatcgtaga cgcggacttc gtcgacgatt ccggtgaacg
    10381 gctccggtcc ggccgtgatc atgccgcgca gcccactgtt gtcggcggga ttgttgccga
    10441 tcgcgattcc gtcgttgccc gttctccaaa tgtacgggcc gctagcgctc gtcatgtcga
    10501 ccgcggtgcc agcgttgtcg tagatgaaga ggttcatgcc cgccgcatcg ctcgtgcaaa
    10561 cgacgtagct ccacacgccg gtcgcgatcg tgcgcgtcga gtcgagtacc ttggatctct
    10621 gcactgcggt gctgttgcgc agagcgcagc ggaggatgcc tgtggcggtg atcccgaggg
    10681 agacgccgtt ggattcaccg ccgatttcgt aaacgacgcg atacgtggtg ccggcgccca
    10741 tcgggaagat ccaggcgctc gccgagacac catcgttgtt gctgctcgtc tggaagtagc
    10801 ccgcaggcgc gtacgcgatc gtcgcgcgat cgtcggtggc gtcgaacagg aggccgccgc
    10861 cgaaccgccc cgtcgtccag gtgggatcta aaccgtcggc accggcgcta ttgccgagga
    10921 cggcgttgac catcggggtc gtgaccgagc tcgctgcatt ctggccgacg ccctcgttga
    10981 gcgcgtatgc ggcgatcggc atcgccggga taaacggagc atcggacccg ccggccgcgc
    11041 cgtcgacggt cgcgaacgaa tggcgcccgc atccgccgag cgcgatcagg acagcgaggc
    11101 gcccgagcca catgcgtcca ttgtgcctga ttctgcgccg ctcatcgatg cggttggcgt
    11161 tgcagaagct cgaagccgcg gacgagatag gcgaggcgtg cttcctcgac ggcatgatcg
    11221 cggacgagaa agcggtgtgt cgtctcgccg tcgaacatca tgatgatgga cgtcacgagc
    11281 atgtcgaagc tcgggccgag cgccgtcgcg acgtcgggca agagctcgcg cgcgagcgcg
    11341 gtgatgttcg tgaagtagtg acgaaggacg ggctcgagcg ccttgcgcag attctcgttc
    11401 gtgcgcgcag cgacctcgag ctcgtaccaa gcttcgttga gtcgagagcg acaggcggcg
    11461 cggacaagga gcaatgccgc ttcgatgtcg gtgttgcgct tcaccgcgga cgcgaacttg
    11521 cgcttgtagt cctcgaggat gcgcgcgcca acgtcggcgc cgactgcaac catcagctcc
    11581 tcgcgcgtcg cgaagtggcg gaacagcgcg ccttgcgaga cgcccgctcg ctcgcagatc
    11641 cgctgcacgc tggcctcggc gtagccgaca tcgatgagcg tctgcgtcgc tgcgtcgagg
    11701 agcttgcgaa tcgtgccctc gcggcgctgt tgctgcgtgc gtcgagctgc aacagggctc
    11761 aagcagcgcg ctccacgtgt gttcgcatga caggaggtgt accgcgtgcc ggcagaaagg
    11821 tgccgtagct cgcggcgtgg ccgagatctc gcgcgaacgt ggtgccgtcg aaggcgacgc
    11881 gcccgttgat gagcaccgcc gcgaccgcac cgtcgctgcg attgaccatg cgcgtgaggt
    11941 cgccgaagcc ttccatgcgt gcctcgtgat aggcgtcgag gcgatcgtcg agcgcggctg
    12001 gatcgacgat ggcgatgtcc gcacgatcgc cgacgcgcag gtgaccggcc tcgataccca
    12061 tccactcgcc gatctcgccg gtcagccgcc acaccgcgcg ctcgatcggc atcgacttcg
    12121 tgtcgcgcac ataccgcagc atccgcagcg cgaagctgta gaacgccatg ttgcgaatgt
    12181 gtgcgccgga gtcggcaaag ccgatgagtg ccgaacgctc ggagatcagc cgcgccatct
    12241 cggccggacg atcattcgcg atggtcgtgc gccagcgaag cgccttgcca tgctgaacga
    12301 cgaggtcgag gaatgcgtcg acgggatgaa tgccgcgcgc gtccgcaatc tcgccgaacg
    12361 acttgccgac gagcgacgcg tccggacagc cgacgatgaa cgcgtcatga aaattgcgct
    12421 gccacacacg tgcggtgccc cggcgctcgt agtccttgcg aaaccgccgg cggtacgcct
    12481 cgtccttcat cagctcgttg cgctcgacct cgctcgcgag atgcaacgcg tcgcggcccg
    12541 cgccgaattc ctcgaagacg accaaatcga tgccgtccgc atacacctcg aagggcacgg
    12601 gcagcgtctg ccaacgcaga ttgccgcgca gcacgcgatt cacgaaccgc gtcatcgggc
    12661 cgatcacgcg gtgcagcccg cgactcgact tcgcatccgc cagcgtgatg agcgtcgtcc
    12721 gcagcgactt ccgccaaaac attccagcgc tcgtcgccat gaacagcagc gcgttgatct
    12781 tcgtgacgat gttcggcgcc gattgcagaa tgcgcccgcg ccggcgcagc accttgtgca
    12841 gccgccgata ctcgccccac gacgcatagc tcgacggcag ctgcttgctc cgaaaccgat
    12901 cgccgtcgag cttgtcccaa ggattcgtca tcgacgagag ccccaagaag cccgcatcca
    12961 acgcgtcctc gagcagccgc tccatctcgc gcatctcgct cgcggtcggc ttcacgctcg
    13021 gatccaccgc gcgctctaac cccatcactc gcgtccgcag atccgagtgc ccgagaaacg
    13081 acgtcacgtt cggcccaagt gcctggcggc gcaagaactc gacgtactcc gccgcgctcc
    13141 tccacgtctt gcgcgccttc aagagcggaa gcacgtgctc gcgcgggacc gactcgacgc
    13201 gcgtaaacaa atccgagcaa tcctcggctt cggccaagac cgcactgatc gaacaactac
    13261 cgaccgtcac ggtcgtcacg ccatgccgca ccgactcgct gagcgaaggc gcggcaatga
    13321 gctccgcgtc gtagtgcgtg tggcaatcca caaagccagg catgacccac ttgccatccg
    13381 catcgatcac gcgtgaacaa ccgcgctcat cgagtggctc atcgctcgcg atcgcaacgc
    13441 ggccatcgcg aatgccgaga tgcttcagcg cccccggcgc acccgtgccg tcgaagtaaa
    13501 ggccgcgctt gatgatgacg tcgtaggcca tggcgagacg ctaacaaaga tagagagtga
    13561 ttgctatcta aatgatgcaa gttcgtagct agcagggctt ttcgcgccgc tccggcgcta
    13621 ctggcggtac gcgaggccta ctgagagcgc gccgtacccg ctgctcgaga tgagcgcgcc
    13681 ttgcacgcgc gccgcgatgc cgagtcgatg cggatgtccc ttccagaggt cgaggccgcc
    13741 ttcgagcccg ataccgagag cgccgtgaag gccacgctca tcgtctgcat agatgtcgat
    13801 cgactgcttg tagagctgat cgagatggac gccgaggagc agggtcgccc agaggcggtc
    13861 gtaggcagtg cgctggatga acgcggcgac gtcgattgga acatcggtga cttcgtagtg
    13921 gttcggtgtc gagcctctgc ccgagtagcg atagccgctc acgtagccga cgccgacgtg
    13981 gaggccgagt gctacggctt tatcgcggcg catcccgagc tcggctgcaa caccagcgcc
    14041 ggtgcagcat tcgatctgga gcgaggcctc ggagctctcg acgaggtggc ccatgatgta
    14101 gaagggcatc agcttcatcg tgaggcgggt gcgcggcgcc tgctcggtga gtgccggttc
    14161 gagatagcta atgtcttctt gctcgcggcg aggcttcgat ttcgcggcgg cttgctcgac
    14221 gcgcgcgagc ggatcgtctg caaccgagtc gcgcttcgcc tgatcgaact ggacgagcgt
    14281 tgggtcgcga tgcagaacgg cgaacgcctg ctttagcgaa tcggctggac cgcgcacgaa
    14341 cacgacaccg cgagtcagat cgagctcggc gagagagccg gcgagggagt cgatcgtgag
    14401 ggcgcgaact tgctgtgcga gcgccacatc actgagcgcc gcacgtccga gatcgacgtc
    14461 gtgctccacc cactcgccga gagcctccgc gcttgcggtc aacgcgccga ggcgaacgag
    14521 cactcgccgg cgtgcgatca cgaacgcctg tgcggctgcg cccgcgtcgg tgtgcagctc
    14581 cgcgatgcgt tgcgcgatta gctcggccgc tgcgggggcg cgcgattgat cgacccaacc
    14641 ggcgaggacg tactccgttg cgaggcgctc ctcgccgagg attgcgtgga ctccgtagct
    14701 cgcgccgagt tgctggcgga tgtcggcagc gatgtccgcg agcatctgcg cgacgacgag
    14761 ctgagcggca cggttcgtcg acgccgtcgg cagcgcgatg cgcagctgcg cctgctggat
    14821 atcttcgacg agaccgatcg atgccgggcg gagcgtcgcg cgaggcagcg gctttttcac
    14881 ggcgtgacct gaccagtcgc cgaataggaa ctcgacccac cgctcggtga gcgctgcatc
    14941 gaacctgcca ctgatcacga gcgtcgcgtt gtcgggcgtg tagtgctcgc gtcggaagcg
    15001 ctctgcatcg gcatcaccga gagtggccgc catgtgacgc aaaccggtcg ccgtgtacgg
    15061 gtgccccgag ccgtagagcg ccgcgttcca cgtgtcagcg actgtgctgt cgacgtcgat
    15121 ttcttccttc gcagcggccc gcatcgcgtc gaccagacct tcggtgccac gttgatacgt
    15181 gccatcgcgc acccaccggc gaagcccggc gagcaggtag tcgatgtgca tgtcgatgcc
    15241 ggcgctagag aaggtcgtgt gatcagtcgt gactttgatg tcattcgagc ccccggcggc
    15301 cgcgaacgcg agcaggtcat tgatgtagcg gatgttccag ccgagaccgt acgctgcgag
    15361 ctttgcggca ccgtgcttat cggctggatc gtcggcggtg cccgacgaga agacgagccg
    15421 cacgtcgacg gtcgggacgc tattcagcgg caagaggacg acctgcatgc cgttggcgag
    15481 ccgcttcgtc gtgatgccgg cgacctcggg caccgcgacg tccgccggtt gccgcgcctt
    15541 tgccggatcc atcgtggtgc ggcgttggcc gacgtcgtga atgtcggcag cgagctcgac
    15601 cttgtggccg cgtttcttgc cggtctccgg tttgagcgtc acgaacgacg caccttgaat
    15661 gctcaagtag cgagtcacga gatcgcgtgc cgtgccctgc gtcagctggc gcagaccttc
    15721 gaactcggcg gcgagcgcag cgctcgggtc gcgccctgca agcgcgaagt ccgcgagcat
    15781 ttgatcgcgc gacggagggt cctcgagcga tcgatacagc cggtagatcg cacgctgccg
    15841 cacataatcg aagccgatct ggtcgagctc ggtgagccgc catttcggat cgaacacgtc
    15901 cgagagctct acgatcgtcg actcgacctt ctccttcacc ttccatggcg actcgtcgct
    15961 tgccggaaat gccacgatgc cgatcacgcg agcaccctcg tcgcccagct cgatcgacga
    16021 gacgcttccc gaaatctcgc cattgatcag atacggcagc gccgccgcaa tcgcgcgcac
    16081 ctgcgtccgg acgcccggat cgattggcaa cggccacgtt acgaggagcg cttcgtcgtc
    16141 gagaggcgcg gttgcgtccg cgcgatgagc ggtggtgggt agggccggga catagcctga
    16201 cggcgcaacg cgacgtcgcg ggatcttcgc gacgagcttg ctcagcgcta cttcgacttc
    16261 ttccggtgtg agattgcccg cgatgacgag cgcagcattc tccggcgcgt aatgcgcatc
    16321 gatgaacgcg cacacatctg cacgctgcaa cgcatggagc gattcttccg aaccaccgat
    16381 cgattgccgg tatggatgcc cgtcgggaaa taccgacgcg ttgatcgcag acaagagttg
    16441 cgtcgagtcg tcgcgctcgc ggatctcgtt gacgacgacg gcgcgctcgc gctcgaagat
    16501 cgcatcggtg atcgtcgtgc agcgcagctg cagacgcagg ccttcgatgg agagcagccg
    16561 atcgagctcc gcacgcggtg cgcgcgacag gtacgtcgtc gcatcgtacg tcgtcccgcc
    16621 gttgaagtac gttgcgtgct cctcgagctg cgtgaacagc gactcgttcg cgatcacctg
    16681 ctggtacatc aggtgctccg ccatgtgcgc gatgccggga tgcgcaccat cgtcgatcgc
    16741 accgacggca tatcgcattg tcacgctgac ctcgggggcg cgagcgtcgc gaaccaagac
    16801 gacacgtaac ccgttaggca actgcgacat cgacactgca agcccgagat ccgtgcggcg
    16861 cggcggcgat ggcggcggcg tggctcccaa tgcgcagcct gcgagcaggc acaacccgaa
    16921 gaccctcaca cgtcgagtat aagatcgaag catgagaacc ttcgcgctcg tcgtcctcgt
    16981 cctcgccgcg tgcggcaaga aatctgactc cggagctgcg gcctccggcg gtgaggtcgc
    17041 gtcctgttac agcgagtcga tgcactcgtg tcgcgagtat cgcggcatga atctcgcgct
    17101 cggcagcgac tcgctgacca agctttgcac cgccgtcgac aagaccgcga agttcagcga
    17161 cacgccatgc gcgacagccg gcgtcatcgg cacgtgcaag atggacgcgg gcaaggactt
    17221 cttctacgac ggttacgtgc tcgcttcgtc gatggaagcg gactgcaagg cttccagcgg
    17281 aacgttcgcg aagtagtcag tcgagctcga acgtgtagat gcgatcttcg ctatcgctca
    17341 cgatgtagag cgtgttgccg gagaaggcga ggccctcggg cttgtcgacc ggcagcttcc
    17401 acgcgcggtc tgggtcccag tgcctgtcga ggcggaacag cgtgtggtct tcgtcgctga
    17461 gcacgtagat gtgcttgtcg gatgcgttgt acgagatgcc tgagaggtct ttggcgaagt
    17521 cgatcttctt ggagtcgacg agcgtgccgt cggtctcgag ctcgtagatg cgggcggggt
    17581 ctttctcttt gaccgcgaac aggcggtcgt cgggcgtcgt gacgacgatg ccttcgatgc
    17641 cgctgttgcc gtcgtcggcg ttgtcgacct cgatcgagtc gtggcggtcg ccgtcttcgt
    17701 cgatcgtcca gatcttcgct ttgagctcgt cggcgatcag gaattcgttg cgcgtgaagt
    17761 cgtaaccgag cgcttcgagg tcgtggccgt cgatgtcgag gtactcgtcg gcgtcgccgt
    17821 cgggcgtgat ctcgtagatc ttggagtgct ggtcgctcac cgcgaacagc ttgctgccga
    17881 cgcgcacgag atcggaaggc tcctccatat cgaggcgcga gcgatcgaga tactcgagat
    17941 gcgcggggtg gccgtccgcc ttgttcagat cgaggaaggg ctcgatcgtc gttggcgctt
    18001 cggcgcaggc cgcgagcgtg acgagtaggg cgcagggcag acgcatccgt gcgaaccgct
    18061 cagcaactgt tgcgccagcg aaatctgtaa tgaatctgca accggtggct acaacaccag
    18121 ccgatcgcgc cggctaggtg acaagcctgc gctcgccgca acgtggtgat gtcgcttgac
    18181 cccagcgcgg cacgaggctt gtagaggtgc ggcggcatgc gcgcgccact catttgtcta
    18241 ctgctcacgg ttggttgcac tgaggtgcca ggggacgcaa aggacgattc gtttggcggc
    18301 tccggcgcga aagaggacgg ccagtactcg gcctgtcagc tcgccgaagt gctcaagctc
    18361 gtcaacgact cgagctcgac ggcgtcgagt ctgcaggaca aggtcggcct cgccgagaat
    18421 gcatcgcgcg cgatcgccgc gcaccgcaat ggcgccgatg gccagcaggg cactggcgat
    18481 gacgacatct acgacgatct gaacgagctc gacggcgtcg acttcgttgg caacctcgcg
    18541 ctcggcaagc tcgtgtacgc ggtgctgccg aagtgcgaaa acgatctgac gggccggccg
    18601 ttcatcgacg atcagacgtt tacgggaccg agcagcggct gggcgcggga caacgccgag
    18661 gtcgaagtcg tgctcggcgt gaagggcacc acgggccaga agcttcgcga gctgctgctg
    18721 cagaagaaca gcgacggccg cacgctctac gatcgcatgc gcaaggcgcg ggtcatggag
    18781 gcgttcacgt atggcttctc gctcgacgag atgccgtggg acagcgacgc gcaagccgcg
    18841 cgcgagaaga tgccgtacgt ctcgctgacg atcgagcccg atcgctatgc gccgaacgac
    18901 caaggcgaga tggagatttc gctcggcacc gatctgatgg acgacacgta ctacgacacg
    18961 cacggctaca cgctccttgg caacgacatc gagctgcgtg cgcgcgcgcg ctgggacaac
    19021 gcaacgaccg ttcgccgcat cttgatcgcc gcgaagttcg gcaccgagat cgacgccgac
    19081 ggcaacaagg tcaacgcgaa ggtcgatatc cgcaacgaca acgggacgag cttcgtctcc
    19141 aagctcgacg acgacgttcg ccgcggcaag acggcgtgga acggcagcga tcaagccgcg
    19201 acgcccatcc gtggcgtgta cgagcagctg atggcgaaga acctcctgct cgatatcggc
    19261 gcgcatcacg gcgtgctcct gctcgagccg gcggttcacc tccgttcgac gcgcagccgc
    19321 tatcacatga acgaggcgaa cattacgaac gttcgcgcga tctacacgaa cgccgtcacg
    19381 cgcatcaacg ccgcgctcgc catcgtcgcg aaggcgaaga cggccggcat catcccggcg
    19441 aaccagctcg ctgccgtcaa cgcgatggag acgctcggca agggcatcgt cgacaagtcg
    19501 ctcatcgccc agcggctcgg cacgaccgtg acgtcgctcc ggctgcccga caactgggcg
    19561 cagccctcga cgccagcgga tctcgcggcg aacaagaaga ccgctgaagc gatcaacgcg
    19621 ctctatcacg agttcgcggc cgccctcgac gacaccgacc gcgcgatcac gaacgccgcg
    19681 gatgaagact tcgacgcttt caccgagatg ttccgcatgt ggcggatcag cgtcgagtcg
    19741 tcgttcgctc gcaagcgctc gtgggacccg ttcctgaatt c
//
LOCUS       DATLBZ010000475         4700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4310645, whole genome shotgun sequence.
ACCESSION   DATLBZ010000475 DATLBZ010000000
VERSION     DATLBZ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4310645"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VMZ53_24120"
     CDS             complement(<1..441)
                     /locus_tag="VMZ53_24120"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HUS31623.1"
                     /translation="MAIKKVESIWVDGQLVPWDQATDHLLAHTMHYGVGVFEGIRAYH
                     RADGRTMIFRLREHIERLIDSAAICTMDPTFTRDQLMQACVDVVKTNKMTSCYLRPLM
                     YLGYGALGLGSFEPPVRTMVACYEWGAYLGDDGLKKGIRCMISGF"
     gene            complement(539..3418)
                     /gene="glnE"
                     /locus_tag="VMZ53_24125"
     CDS             complement(539..3418)
                     /gene="glnE"
                     /locus_tag="VMZ53_24125"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HUS31624.1"
                     /translation="MVRPADLAIGAPDEGDAGRRAERLFDAASQSGLDLTDLDETAVQ
                     VATLACQRAPYLATLLTRDPHRLGRVAQDPYLRREKPLDQLMGQARAALSGVTTGAEL
                     RTALRRIRADELVRLGVRELELGLDTEVGRELSRLADVCFDAAIDFHDRELRARYGAP
                     MYEDDDGTQREAKLAVIGMGKLGGEELNFASDVDVIYVYSSDHGEVGGLSLHEYFAKL
                     CTLVTGALADVTEDDVVFRVDLRLRPEGAKGAIANSLAQMERYYETFGRPWERQAWIK
                     ARACAGDPALGAETLEMLKPFVFPRYTSPAIIEEVHALNRRIKRELVKTRDGFDLKNG
                     DGGIREIEFFAQALQLIHGGKRLSLRCRGTLAALDALQFAGLVSDDEHLSLWRAYRWL
                     RHAEHVLQLEAGLQTQTIPADPELQDVFARRLGYADHHGLQRILAAHTGSVARMFATL
                     GVEVDEGRPDVDAILRDELSDEDEAAALARLGFRDVTAAIAELARARRRPGSPLSPAA
                     SERAARIGSALLSEIANSADPDQALRALGDLVARRGEAWSIWRLFDEQPAIVRLLGSI
                     FGASAYLARTLVDTPELIDILVELGQSAPTRTVAQVAADLDAKLAAVDKSDPEALWSA
                     VAEVKAAHVLRVGLADFAGALDPLAVCVELTAIAEACLQTALTIVEQQMPKRAGALSV
                     LALGKLGGYELGYAADLDVVFVYEGGDDDEAVVHYSRLAQRLLGALRQRTPRGRLYEV
                     DTRLRPSGSQGLLVSSLVAWRRYHEHDARLWERQALIKLRPVAGDKALGAEVARLAAE
                     TVYGKSHDRKAVAEEIGAMRTKIERELGGGAHKRVGGDLKTDAGGVIDVEFAAQFLQL
                     IHGHAHAALRTTSTSTALRAAASLGIAPEGLVDLLDQGYRFLRGIEHRLRVVHDQPIH
                     RLPDARDELDRLARRSGFPDGAALVERVERWQHDIRAAYRTLLGA"
     gene            3489..4601
                     /gene="dnaJ"
                     /locus_tag="VMZ53_24130"
     CDS             3489..4601
                     /gene="dnaJ"
                     /locus_tag="VMZ53_24130"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HUS31625.1"
                     /translation="MRDLYEVLGVERGASQADLKKAYRRLAQQFHPDKCGGDKEAEEK
                     FKEAANAYQILSDDEQRAAYDRYGFDGIRRGAGGPGPGAGFSNVEDVFSAFGDLFGDF
                     FGGRSSGKRQPRGADLRVDLQLSFAEAVWGCTKDVQVTRAIACTTCSGSGAAKGSQPE
                     VCRTCQGKGQVVHSQGFFMVQSTCPHCRGAGKQIKDPCEDCRGRGLKPETSTLQVTVP
                     AGVDDGQTLRLAGKGESVPNGATGHLYVVLHVQGDERFKRDGDDILTEIPISFVKAAL
                     GGEVEVYTLEDNCNGTATVELKPGTQPGDVIVRRGQGIQHVGEHGRGDQVIMFKVEIP
                     KKLSARQEELLRELATELNEDGVRPEKRGLFGRTKK"
BASE COUNT          818 a         1552 c         1574 g          756 t
ORIGIN      
        1 gaagccggag atcatgcagc ggatgccttt tttgaggccg tcgtcaccga ggtacgcgcc
       61 ccactcgtag caggcgacca tcgtgcgaac gggcggctcg aatgagccga ggccgagtgc
      121 gccgtagccg aggtacatca gcgggcgcag atagcaggac gtcatcttgt tcgtcttcac
      181 gacgtcgacg catgcctgca tcagctggtc gcgcgtgaac gtcggatcca tcgtgcagat
      241 ggcggccgag tcgatcaggc gctcgatgtg ctcgcgcagc cggaagatca tcgtgcggcc
      301 atccgcgcgg tggtaggcgc gaatcccttc gaacacgccg acgccgtagt gcatcgtgtg
      361 cgcgagcagg tgatcggtcg cttggtccca ggggacgagc tgcccgtcta cccagatcga
      421 ttcgaccttt ttgatggcca tgtccgcttc gtagcaagag gcagggagag atcacaaggc
      481 gcagtgcatc aaggatctcc cgaccacaga tcgtgacgcg ctgcggctgc gaatgaagct
      541 acgcaccgag cagcgtgcga tatgcagcgc gaatgtcgtg ctgccagcgc tccacgcgct
      601 cgacgagcgc agcaccgtca gggaagccag agcgtcgtgc gagccggtcg agctcgtcgc
      661 gtgcgtccgg caaacgatgg atcggttggt cgtgcaccac gcgcaggcga tgctcgatac
      721 cgcggagaaa tcgatagcct tggtcgagta agtcgacgag gccttccggc gcgatcccga
      781 gcgaggccgc tgcacggagc gcagtcgagg tgctcgtcgt acgcagcgcg gcatgcgcgt
      841 ggccgtgaat gagttgcagg aactgcgccg cgaactcgac gtcgataacg ccgccagcgt
      901 cggtcttgag gtcaccgccg acgcgtttgt gcgcaccgcc accaagctcg cgctcgatct
      961 ttgtgcgcat cgcaccaatc tcttcggcca ctgcttttcg atcgtgagac ttcccgtaga
     1021 cggtctctgc ggcgagtcgc gcgacctcgg cgcccagtgc cttatcgcct gcgacgggcc
     1081 tcagcttgat cagcgcttgg cgctcccaga gccgcgcatc gtgctcgtga tagcgacgcc
     1141 acgccacgag cgagctgacg agcaagccct gcgatccgct cggccgcagg cgcgtgtcga
     1201 cttcgtagag gcggccgcga ggcgtgcgtt ggcgaagtgc gccgagcaag cgctgcgcaa
     1261 gccgcgagta gtgaacgacg gcttcgtcgt cgtcgccgcc ttcgtagacg aacacgacgt
     1321 cgaggtccgc cgcgtagccg agctcgtagc cgccgagctt gccgagcgcg agcacggaga
     1381 gtgcgcccgc gcgttttggc atctgctgct cgacgatcgt aagcgccgtt tgcagacacg
     1441 cctcggcgat cgccgtgagc tcgacgcaca ccgcgagtgg atccagcgcg cccgcgaagt
     1501 ccgcgagacc gacgcgcagc acgtgtgccg ctttcacttc ggcaacggcg ctccacagcg
     1561 cttcggggtc gctcttgtcg acggcggcca gcttcgcatc gagatccgcg gcgacttgcg
     1621 cgacggtgcg cgtcggcgcc gattggccga gctcgacgag gatgtcgatc agctccggcg
     1681 tatcgacgag cgtgcgtgcg agataggcgc tcgcgccgaa gatcgagccg agcaggcgaa
     1741 cgatcgcggg ctgctcgtcg aagaggcgcc agatcgacca tgcctcgcct cgtcgtgcga
     1801 cgagatcgcc gagcgcgcgc agggcctgat ctggatccgc gctgtttgcg atctcggaga
     1861 gcagcgcgct gccgatgcga gcagcgcgtt cgctcgcagc gggcgagagc ggagagccgg
     1921 gacgccggcg cgcgcgtgcg agctctgcga tcgcggcggt gacgtcgcga aagccgaggc
     1981 gggcgagggc ggcggcttca tcctcgtcgg acagctcgtc tcgcaagatc gcatcgacgt
     2041 cgggacggcc ttcgtcgact tcaacgccca gcgtcgcgaa catgcgcgcg accgagcccg
     2101 tgtgcgcggc gagaatgcgc tgcaggccat gatggtcggc gtagccgagc cggcgcgcga
     2161 acacgtcttg gagctcgggg tccgcgggaa tcgtctgcgt ctgcaggccg gcctcgagct
     2221 gcagcacgtg ctcggcatgg cgaagccagc gatacgcgcg ccacagcgag agatgctcgt
     2281 cgtcggacac gaggcctgcg aactggagcg catcgagggc cgcgagcgtt ccgcgacagc
     2341 gcagcgacag ccgcttgccg ccatggatga gctgcaacgc ttgcgcgaag aactcgatct
     2401 cgcggatgcc gccgtcgccg ttcttgagat cgaacccgtc gcgcgtcttc acgagctcgc
     2461 gcttgatgcg gcgattgagc gcgtgaactt cctcgatgat cgccggcgac gtgtagcgcg
     2521 ggaacacgaa cggcttgagc atctcgagcg tctcggcgcc aagcgctgga tcgcccgcgc
     2581 acgcgcgcgc cttgatccac gcttgccgct cccacggtcg accgaacgtc tcgtagtagc
     2641 gctccatctg cgcgagcgag ttcgcgatcg ctcccttcgc accctccggc cgcaaccgca
     2701 gatcgacgcg aaacacgacg tcgtcctcgg taacgtctgc gagcgcgccg gtcacgagcg
     2761 tgcacagctt cgcgaagtat tcgtgcagcg agagcccgcc gacctcgccg tggtcgctcg
     2821 agtagacgta gatgacgtcg acgtccgacg cgaagttcag ctcctcgccg ccgagcttgc
     2881 ccatgccgat cacggcgagc tttgcttcgc gctgcgtgcc gtcgtcgtcc tcgtacatcg
     2941 gtgcgccgta gcgcgcgcgg agctcgcgat cgtggaagtc gatcgccgca tcgaagcaga
     3001 cgtcggcgag gcgcgagagc tcgcggccga cttcggtatc gagacccagc tccagctcgc
     3061 gcacgcccag ccgcacgagc tcgtccgcgc gaatccgccg cagcgcggtc cgcagctcgg
     3121 ctcccgtggt cacccccgag agggcagccc gggcctgacc catcagctgg tcaaggggtt
     3181 tctcgcgccg caggtatgga tcctgcgcga cccggcctaa tcggtgaggg tctcgggtca
     3241 gaagcgtcgc gaggtacggt gcacgctggc aggccaacgt cgcgacttga accgcggtct
     3301 cgtccaagtc ggtgagatca agcccgcttt gcgacgccgc atcgaacaat cgctccgcgc
     3361 gccggcccgc atccccctcg tctggggcgc cgatggcgag atccgcgggc ctgaccatgc
     3421 ggtcaccata ccttgacacc cacgcggtcc cgcattaccc aaagccttga ttctcctcag
     3481 cctgcgtcat gcgtgatctt tatgaagtac tcggggtcga gcgtggcgcg agccaagccg
     3541 atctgaaaaa ggcgtaccgg cgcctcgcgc agcaatttca tcccgacaag tgcggcggtg
     3601 acaaagaggc cgaggaaaaa ttcaaagaag ccgcgaacgc atatcaaatc ctttccgacg
     3661 acgagcagcg cgctgcgtac gatcgctacg gcttcgacgg cattcgtcgt ggcgctggcg
     3721 ggcccggtcc cggggctggc ttctcgaatg tcgaagacgt cttctccgcg ttcggcgatc
     3781 tgttcggcga cttcttcggc ggtcgctcga gcgggaagcg tcaaccacgc ggcgcggacc
     3841 tgcgcgtcga tctacagctc tcgttcgcag aggcggtgtg gggctgcacg aaagacgtgc
     3901 aagtcacccg cgcgatcgca tgcacgacgt gcagcggatc cggcgccgcc aaaggcagcc
     3961 agcccgaagt ctgccggacc tgccaaggca aaggtcaggt cgtgcactcg cagggcttct
     4021 tcatggtgca gtcgacgtgc ccgcactgtc gcggcgccgg caagcagatc aaagatcctt
     4081 gcgaggactg tcgcggtcgc ggtctcaagc ccgagacgtc gacgctgcaa gtcacggtgc
     4141 ccgcaggcgt cgacgatggg caaacgctgc ggctcgcagg caaaggcgag tcggtgccga
     4201 acggcgcgac cggtcatctc tacgtcgtgc tccacgtcca aggcgacgaa cgcttcaagc
     4261 gcgatggcga cgacatcctc accgaaatcc cgatcagctt cgtcaaagcg gcgctcggcg
     4321 gcgaggtcga ggtctacacg ctcgaagaca actgcaacgg gaccgcgacc gtcgagctga
     4381 agcccggcac gcagcccggc gatgtcatcg tgcgacgcgg ccaaggcatc cagcacgtcg
     4441 gcgagcacgg tcgcggcgat caagtgatca tgttcaaggt cgagatcccg aagaagctca
     4501 gcgcgcgcca agaagagctg ctgcgcgagc tcgcgaccga gctcaacgaa gacggcgtgc
     4561 ggcccgagaa gcgcggcttg ttcggtcgca cgaagaagta gcgctagtac ttcgggacgt
     4621 acagcttcag ctcgacgcgg cgattcgcga ggcgcccctt cgccgaaccg ttttcggcca
     4681 cgggctcctc ggcgccgacg
//
LOCUS       DATLBZ010000476        15652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8503399, whole genome shotgun sequence.
ACCESSION   DATLBZ010000476 DATLBZ010000000
VERSION     DATLBZ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15652
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8503399"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2574)
                     /locus_tag="VMZ53_24135"
     CDS             complement(<1..2574)
                     /locus_tag="VMZ53_24135"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HUS31626.1"
                     /translation="MESGVDIDESRLRALAGPAYARGLAYLRDGAVRSCTVVGDSIEG
                     EVVGSETYKVRLSFAAGSIDASCTCPVMYEVCKHAVALALHYLGEKPIDVPSTEGTFA
                     TKADVDAFVTEHRIEHALMLSAELLIARIALPTHEYWVRGLLARLAVRDVASLEGAAR
                     HARTRELDGGLAKASYDLLHEQAAIVADGLAEESVRPVTPAAVAPLWTKLVDVRRKLR
                     EVASPRSRAWRAAQTWTFDAASATITWKERERICRPRDFMTQAVTTRFTMPGGGAPKL
                     ECTCGGKPSLNESSFTQCVHGLSLVDATLDVLADPAKEPVAKQMVDELLKPAWSRAIA
                     ELDRIDERAAKPRIAIEVWWHIEYELGALNLSPTVKKQMKRGGMSSGQRTSPHRMLEE
                     HGEVLSEPDRRIAELLTAWMPGRGSTYPARAFAALVGHPRIISEHDDEPIDLVRTELG
                     FAALGEGDKIRLEPSLFGSRMSPRMLGSLLQIHPPGEPLFVPEPERRRCVLVDVSDDA
                     RQLWSVLDKHGDMFPPEAHAALLDRLGKLEARLPIDVSESLKGRAVTADPTVVVRVRL
                     LPDVTLEAELFVRPGPGAPLFSPGSGPRDVMVPVGNGRGYIRRDLGNELGFARSMFAR
                     LPIIDAEEGPPQCFRFGNPDDAIAFVDALQSPPEGIEAEWVDRKKPPTVSRAAAEKLR
                     VYVDKKRDWFGIAGDLRIEAGRLELAVILDAARRQQKYVRVDDNRWVELSHTLRERLQ
                     AVADRTFSASRTKDALELSPSAVLAIRALAESGVHVEVAEPWRDLTQRVLAAASFKPK
                     RPRGLTGILRDYQVEGHAWLARLASWGAGGCLADDMGLGKTVQAIALLLDRSKLGPAL
                     VL"
     gene            2600..3454
                     /locus_tag="VMZ53_24140"
     CDS             2600..3454
                     /locus_tag="VMZ53_24140"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUS31627.1"
                     /translation="MAIRGYYLGCPGWGMKTWVGRLFPPGTRNQDFLARYAEVFNTVE
                     GNTTFYALPTADTVARWNEQTPSHFRFCFKFPREITHDQLLVDPDAHLATFLDRIAPL
                     GDKIGTLFLQLPPRFDATQLPRLRGFLDALPRGPHYAIEVRAPEFFLRGPAETELLAL
                     LRERSVDLVILDTRGIHSSKSLTHAETRARKPNLPVIMRATANQPFVRCVPHEDFAQT
                     SDIVEAWATQVAGWIAEGKTPYFFMHAPDDTFAPENAYGFHARLAARSDVGDLPAWPG
                     QPRQLGLF"
     gene            7507..8547
                     /locus_tag="VMZ53_24145"
                     /pseudo
     CDS             7507..8547
                     /locus_tag="VMZ53_24145"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            8551..8655
                     /locus_tag="VMZ53_24150"
                     /pseudo
     CDS             8551..8655
                     /locus_tag="VMZ53_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            8671..8772
                     /locus_tag="VMZ53_24155"
     CDS             8671..8772
                     /locus_tag="VMZ53_24155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS31628.1"
                     /translation="MDSGCGCSGGGNPAGSWPLLGAIGLLFRRRRRR"
     gene            complement(8769..11021)
                     /locus_tag="VMZ53_24160"
     CDS             complement(8769..11021)
                     /locus_tag="VMZ53_24160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31629.1"
                     /translation="MAINSEIQQLIASGQHLEAARRAQAEGDHALAADLYEKLWDFRS
                     ALDAARGAGDLPRALRYALELHDDATAKGLLDELNATPEGSKAALDMLVRMRRHAEAA
                     PIAEKLGDVNKAIDLFTRARKELEAARLLESQGRDRDAGRLIERALDLASPSEKAPLQ
                     LALGRILARRGAYPEAARLLQESRKQPELRADAQRHLIATLAAMGLRDGARDALLELR
                     AVDQSVPADLDTYLRAWRDESTERKTSRDREVIAGRYRLEKLLGSGASGRVFLATDEV
                     AGRQVAIKMFFASGARGGAAYERFVREARLASTLRHPSLVEVYDVSVERGFLVMEYLP
                     GGSLAARLGAGERLTAPQVRRMAFDIIGGLEAAHHRGVVHRDVKPANIFFDARGTAKL
                     GDFGVAHLVDLGQTQTGGLIGTLAYMSPEQITGASISISADLYALGVTLFEVITGRLP
                     FLGPDFVAQHLGEEPPVASSVAEGIPQAWDGMLAALLVKNPQERTQTLADLRHQLETL
                     DLHGQPRVGPRPRRSSAPHSIAQLAEEGEAKPRYQFETQLGVTTISQLSRAVDTVLDR
                     SVIIERFDQTDDALRAVERARMLGRAQSPFVQRALSLDRGARVAVFEAPSGASLADSP
                     PDLPATEALRVLKRLARGAAAIHIGGGSHGGIAPRFVVLDDGAAPTVMIAGLGVIGES
                     QPLDDVNAIIGVMATLAGCEPSFDAFAKSMCDEVGAAVPSYTMPLDAESLFAAADIVD
                     IAVLAALGSR"
     gene            11051..11989
                     /locus_tag="VMZ53_24165"
     CDS             11051..11989
                     /locus_tag="VMZ53_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HUS31630.1"
                     /translation="MHQRSAHRSLLVVGSVAFDDIDGPFGLHKDLLGGSASFITRAAA
                     YFTRNVSVVAVVGEDFPQKYVDEMSQQGIDVSGIERTPGETFHWVGKYSPDLSSRETI
                     DTRLGVFATFQPKLLDAHKDAQLVLLGNIDPELQLDVLRQVRAPALVALDTMNLWINI
                     KRAELEKTLSKVHTLMINDEEARLLAGDHNLRKAAARILKMGPTSVIIKRGDAGALLF
                     HGGGVFAAPAMQLEDVKDPTGAGDSFAGGLMGYLAYAGDLSPKSIRTAMIMGSVMGSF
                     AVEKFSVDGLRDLTAAKIQQRFDEMAELVHFEKVRL"
     gene            12034..12462
                     /locus_tag="VMZ53_24170"
     CDS             12034..12462
                     /locus_tag="VMZ53_24170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31631.1"
                     /translation="MRLRWLVVAASVAACSREPAAETGPSFRRDIAPVLEKHCTAKEC
                     HGAEPAPDVDLDLRAPFAYRQLVGVPAEMGEIRRSRVVPYDVYGSFLVHKLAGPSGPR
                     EGKRMPLDPATGEPLERSPLPSRFVDDVIVKWIEEGAPDN"
     gene            complement(12463..14514)
                     /locus_tag="VMZ53_24175"
     CDS             complement(12463..14514)
                     /locus_tag="VMZ53_24175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31632.1"
                     /translation="MSDHASSDALARFVDDSLAAEERAALEEHLDTCDDCRSTVAMLV
                     RAAVVERRGSEPTLGTHPPEAFQPTEVSTSMPQGPRSSLPKGTKVGRYIIGDTLGLGG
                     MGVVYAAHDPDLHRDIAIKVMRPEFSAANPDATRRIVREAQAMARVSHPNVVSVYDVG
                     THEGQVFIGMERISGQSLREWLTSSRTTAEILEVFIGAGHGLIAAHNAGMVHRDFKPD
                     NVLVDAAGRARVTDFGLAFDQADGESVDTSAEGMTRPIVGTPAYMAPEQHAGTNVDPR
                     SDQFSFAVALYEALYGSRPFAGKSRDELATSILEGKVEPPPAGTHVHASLRAILQRAL
                     STKPGDRFASMEDLLHALARDRGRQPRRVAFGALVLFALVAVGFGADYVLRERTHAVT
                     RGSFEAARAQVDKLFALRTDAFIAQADAMYRLPIMLEIATARDQSDFGLGDEAEDRAR
                     IARQHETLQSADWVGLVRSRHGDVLAIADHKGRLLYDSVSPELFGSSVTEIAPLAAAY
                     SSQTETYLGVIDGADPAVVRSGLLGGVPQRKLYVVFARAKRVGAVPSSMFMQLVEAGR
                     LLDEVGVGQGTLLSVVTAEGIAEGQVPKSVLLAVHDRGISELSVDDGTWLAERTPLRT
                     RGQDSAIAQLVLARQTNVGLSGLFPHARQTLAALAALLIGIALAGFVLARKRDVNRRS
                     R"
     gene            14544..15434
                     /locus_tag="VMZ53_24180"
     CDS             14544..15434
                     /locus_tag="VMZ53_24180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HUS31633.1"
                     /translation="MSNLAAVFLDARGETASDADRGALEGALSDAVTAATSGWPTVRV
                     DPAAWIRALAELAPKPLDLAKPLGEVLVADHYLAFACAAGDPAAVAECNAILVREAGF
                     AADGTKMHASVRDEATQIVRATLLAPREGKPPAIGDYAGRGALRSWLRVCVSRELVRI
                     AKAQQRIEPLEEHLVADPGYGDDPALEELKAKYRAQLADAFRTALTELPARERTLLRY
                     QLIDGLTIDEIGGIFRVHRATAARWIAKIRDDLVMRTREMMAASLGVDTSEAASIVRL
                     VQSQLDVSVIRHLGPPPKKR"
     gene            complement(15440..>15652)
                     /locus_tag="VMZ53_24185"
     CDS             complement(15440..>15652)
                     /locus_tag="VMZ53_24185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31634.1"
                     /translation="SFRIDHVPPGTWTLFAFSRRAVQPVSQKISVAADGVTEVTLKLD
                     EVKKDFTHPNKYGEKYRPTTVYAPGS"
BASE COUNT         2728 a         5466 c         5046 g         2412 t
ORIGIN      
        1 gagcacgagc gccggcccga gcttgctgcg atcgaggaga agcgcgatcg cctgcaccgt
       61 cttgccgagg cccatgtcgt cggcgaggca gccgcctgcg ccccacgacg ccagacgtgc
      121 gagccacgcg tggccctcga cttgatagtc gcggaggata ccggtgaggc cgcgcggtcg
      181 cttcggcttg aatgacgctg ctgcgagcac ccgctgcgtc aggtcacgcc agggctcggc
      241 cacctcgacg tgaacgccgg actcggcgag cgcacgaatc gccagcacgg ccgacggcga
      301 gagctcgagt gcgtctttgg tgcgtgacgc ggaaaacgtg cgatcggcga cggcctgcaa
      361 gcgctcgcgc aacgtgtgcg acagttcgac ccagcggttg tcgtcgacgc ggacgtattt
      421 ctgctgccgc cgcgctgcat cgaggatcac cgcgagctcg agacgcccgg cttcgatccg
      481 cagatcgccg gcgatgccaa accagtcgcg cttcttgtcg acatacacgc gcagcttctc
      541 ggccgcggcg cggctgaccg ttggtggctt cttgcgatcg acccactcgg cctcgatgcc
      601 ttcgggcgga gactgcagcg cgtcgacaaa cgcgatcgcg tcgtcgggat tgccgaaccg
      661 aaagcactgc ggcgggccct cttcggcgtc gatgatcggc aagcgcgcga acatgctgcg
      721 cgcgaagccg agctcgttgc cgaggtcccg acggatgtag ccgcggccgt tcccaacggg
      781 aaccatcacg tcgcgaggtc ccgagcctgg tgaaaacagc ggcgcgcccg gacccggacg
      841 aacgaacagc tcggcctcga gcgtgacatc gggcaagagg cgcacgcgca cgacgacggt
      901 cggatccgcc gtgaccgccc ttcccttgag actctcggag acgtcgatcg gcagccgcgc
      961 ttcgagcttg ccgaggcgat cgagcagcgc agcatgcgcc tccggcggaa acatatctcc
     1021 gtgcttgtcg agcaccgacc acaattgccg cgcgtcgtcg gagacgtcga cgagcacgca
     1081 acgccggcgc tcgggctcgg gaacaaacaa cggctccccc ggtggatgga tctgcaagag
     1141 cgagccgagc atgcgagggc tcattcgcga gccaaacagc gatggctcga gccgaatctt
     1201 gtcgccctcg ccgagagccg caaagccgag ctccgtgcgc acgagatcga tcggctcgtc
     1261 gtcgtgctcg gagatgatgc gcggatgccc gacgagtgcc gcgaacgcgc gcgccggata
     1321 cgtcgagccg cggcctggca tccacgccgt gaggagctcg gcgatccggc gatcgggctc
     1381 gcttagcacc tcgccatgct cttcgagcat gcggtgcggg ctcgtccgct ggccggagct
     1441 catgccgccg cgcttcatct gcttcttcac cgtcggcgag agattcagcg cgccgagctc
     1501 gtactcgatg tgccaccaca cttcgatcgc gatgcgcggc ttggccgccc gctcgtcgat
     1561 gcgatcgagc tcggcgatcg cgcgcgacca cgccggcttg agcagctcgt cgaccatctg
     1621 cttcgcgacc ggctccttcg cgggatccgc gagcacgtcg agggtggcgt cgacgagcga
     1681 gagcccatgc acgcattggg tgaaggagct ttcattcaat gaaggcttgc caccgcacgt
     1741 gcattcgagc ttcggggcgc cgccgccggg catcgtgaac cgcgtcgtta ccgcttgcgt
     1801 catgaagtcg cgtggccggc agatccgctc gcgctccttc cacgtgatcg tggcgctcgc
     1861 ggcgtcgaac gtccacgtct gcgcggcccg ccacgcacgc gaccgcggcg acgcgacctc
     1921 gcgcagcttg cgccgaacgt cgacgagctt cgtccacagc ggtgcgaccg cggctggcgt
     1981 aacgggccgg acgctctcct ccgcgaggcc atccgcgacg atcgccgctt gctcgtgcaa
     2041 caggtcgtac gaagctttcg cgagcccacc gtcgagctcg cgcgtacgcg cgtggcgcgc
     2101 tgcgccttcg agcgaggcga cgtcgcgaac ggcgagccgc gcaagcagac cgcgcaccca
     2161 gtattcgtgc gtcggcagcg cgatgcgcgc gatgagcagc tccgccgaca gcatcagcgc
     2221 gtgctcgatc cgatgctcgg tcacgaacgc atcgacgtca gctttcgtcg caaacgtgcc
     2281 ttcggtcgat ggcacatcga tgggcttttc gccgagatag tgcagcgcga gcgcgacggc
     2341 gtgcttgcac acctcgtaca tgaccgggca cgtgcagctg gcatcgatgc tgccggcggc
     2401 gaacgagagc ctcaccttat aggtctcgct gccgacgacc tcgccttcga tcgaatcgcc
     2461 gacgacggtg cagctgcgca ccgccccatc gcgcaggtac gcgagcccgc gcgcatatgc
     2521 cggtcccgcc agcgcgcgca gccgtgactc gtcgatgtcg actccagatt ccacgaagcg
     2581 ctgtagtgta gcggacgcca tggccatccg cggctactac ctgggctgtc ccggctgggg
     2641 catgaagacg tgggtcggcc ggctgttccc gccgggcacg cgcaaccaag attttctcgc
     2701 gcgctacgcc gaggtgttca acaccgtcga gggcaacacg acgttctacg cgctgcccac
     2761 cgccgacaca gtcgcgcgct ggaacgagca gacgccgtcc cactttcggt tctgcttcaa
     2821 gttcccgcgc gagatcacgc acgatcagct gctcgtcgat cccgacgcgc acctcgcgac
     2881 gtttctcgat cgcatcgcgc cactcggcga caagatcggc acgctattcc tccagctgcc
     2941 gccgcgcttc gatgcaactc agctgccgcg gctgcgcgga tttctcgacg cgctcccgcg
     3001 cggtccgcac tacgccatcg aggtccgcgc gcctgagttc ttcctgcgag gccccgccga
     3061 gaccgagctg ctcgcgctgc ttcgcgagcg cagcgtcgat ctcgtgatcc tcgacacacg
     3121 cggcattcac tcgagcaaga gcctcaccca cgccgagacg cgcgcgcgca agccgaacct
     3181 ccccgtgatc atgcgcgcga ccgcgaacca gccgttcgtg cgatgcgtgc cgcacgaaga
     3241 tttcgcccaa accagcgaca tcgtcgaggc atgggccacg caggtggcgg gctggattgc
     3301 agaaggcaag acgccttact ttttcatgca cgcgcccgac gacacgtttg cgccggagaa
     3361 cgcgtacgga tttcacgcgc gcctcgcggc ccgcagcgac gtcggcgacc tccccgcgtg
     3421 gcccgggcag cctcggcaat tgggcctgtt ctagttagcc cacgactacc ccgacggggc
     3481 ccgacatttt cggccgcaac gggtttagga tggcccgtgt tctctacacg tcggggtcgt
     3541 gtcctagctg cgcctatggc gctaatcgca gcgtctgtcc tgagctgcaa tgatgtcgac
     3601 aacagtggcg tcgacgcgca cttcgggtcg accacgcagg cggtcacggg cgccaacgat
     3661 gcgccggtgc tcgacgcgac gaagagcccg acgctcgcgc cgatgtactc gacgagcgcg
     3721 gcgccgacgg gcgcagttgg atcgctcgtc tcgaacgtcg tcgattacgc gacgcccgcg
     3781 ggacaggtcg acaacgtcac ggacctcgac gtcaatgaag ccgaggcgtc atcgctcggc
     3841 attgcgatca ccgcggccga cacgacgaac ggcaactggt tctactcgac gaacggcggc
     3901 accgcctgga acccgctcgg cgcggtgacg gactcatccg cacgcctgct cgcggccaat
     3961 tcgagcacgc gcctctactt ccaagcgacc gctggattca gcggcgcagc aacgatcacg
     4021 ttccgcgcgt gggacggtac gtctgcgctc accgacacga caacgaacgg cggcacgacc
     4081 gcgttctcga ccgccaccga caccgcgtca ctcacggtcg cggctccgat cacgttccgc
     4141 gcagcgacga atgcctctac ctcgaacacg gcgtccacca cgctcgtcat caataagccg
     4201 gtgggcacgg cgcagaacga cttcttgatc gccggtatca cgacggcatc gacgacgacg
     4261 atcaccgcac ctggcggttg gaccacgctc gtcgatcaga caagcggtac cgagcgccta
     4321 gcgatcttca cgcacctcgc aggtgcagcc gaaggcgcga gctacacgtt cacgttcggt
     4381 gcatcggtca acgcgaccgg aggcatcacg gcgttcaccg gtatcaacac ggcaagcccg
     4441 atccacgtct ccggcgcagg tgccaccgca tcgagcgtga accaaacggc gccggtgctg
     4501 acgacgagcg tcggcaacac gatgctcgtc acgtggcacg catatgcgag cgcgggcacg
     4561 tggacgccgc ctgccggcat gaccgaggcc gtcgaccgca acagcgacac gggaacggga
     4621 ccgaacggcg tctccatctc cgcgaactat ctcgcgctcg cgaccgcagg cacgacgggc
     4681 acgcgcaccg ccgtcgcgtc gaacaacggc gataccggca tcacggcggc gatcgttcta
     4741 cagacagcga acaacgcgcc cgtgctcgat acgacgaaga gcccggcgct aggaagcatc
     4801 gcggaggatg cggctgcacc aacgggcgcc gtcggcacgc tcgtatcggg cctggtcgat
     4861 tacgacagcc cggcgggaca ggtcgacaac ttcatcgacg tcaatggcac gagccaattc
     4921 ggcatcgcga tcaccgccgt cgattcgaca aactgcacgt ggtcgtactc gctgaacggc
     4981 ggcacgacgt ggacggcgat tcccgcggtg agcgatgcgg ctgcgcttcc gctcgcggcc
     5041 gacgcgaaca cgcgcatcta ctgccagccc acgtcgaact tcaacggaac cgtcgcgagc
     5101 gcgatcacgt tccgcgcatg ggacatgtcc accggcacgg cgggcacgct cgacagcacg
     5161 agcacgaacg gcggttcgac cgcgtactcg accgcgaccg acaccgcgag cctgaccatc
     5221 accgcggtca acgacgcacc gagcgcgacg aacatgagcg cgcccgagac gtacaccgaa
     5281 gacacgccgc tcgatctgat cgatatcgtc atcaccgaca tcgacagcac gacggtcacc
     5341 gccaccctca cgcttgcgaa cacgagcgcg ggcagcctca gcgccggcac cgtcggctcg
     5401 acgacgtcga cgttcacggc cgcgaccggc gtgtggaccg cgaccggccc gatcaatgag
     5461 gtcaacgcgc tgctcgcagg cgtgatctac acccccgcgt cgaacttcaa cacgacgttc
     5521 aacatcacca cgacggtcag cgacggcacg ctgtcggcga cgcccggtac gaaggtcatg
     5581 accggcgtgt cgtccaacga tccgccgagc gcgacgaacc tcaacgcccc cgagacgtac
     5641 accgaggata caccgctcga tctgacggac atcgtcatca ccgacggtga cagcgcggtc
     5701 gtcacggcca cgctcacgct gtcgacggca gcagcgggca cgctgagcgt cgctacgtcg
     5761 ggaactacca cgtcgacgtt ctccggcggc ctgtggtcgg cgagcggccc catcgccgag
     5821 gtcaacgcac ttctcgcggg tgtgatgttc aacccggcca cgaacttcga tgcgaatttc
     5881 acgatcgcga cgtccgtgga cgacggcacc gcggcgccgg tcaccggcac gaagacaatg
     5941 acggcgagcg gtacaaacga tgcgccggtg ctcgatggtt cgaagacgcc tgcgctcgct
     6001 gccgtcagcg aggatgcgcc ggccccgacc ggtgccgtcg gcacgctcgt ctcgagcctc
     6061 gtcgacttcg ccacgcccgc gggtcaagtc gacaacgtca ccgacccgga cgccggcgcg
     6121 cagctcggca tcgccgtcac gtcggtcgac tcgacaaact tcacggtctt ctactcgacg
     6181 aacggcggca cgacgtggca gccgatgggt gcggtcacca acgcgaacgc acggctcctc
     6241 gccgcggacg ccaacaaccg catctacgtg cagcccgttg ctaacctcag cggcacgttc
     6301 gcgaccgcga tcacgttccg cgcgtgggat cagtcggcgg gtaccgacgg cacgctcgtc
     6361 gacacgacga cgaacggtgg cggcaccgca ttctcggcgg gcactgacac gatcaccgcg
     6421 acggtcaccg cggtcaacga cgcaccgacc gcgacgaacc tcagcgcacc cgagacgtac
     6481 atcgaggaca cggcgctcaa cctcacggac atcgtcgtga gtgacgtcga cagcgcgaat
     6541 gtcacggtca cgctgacact ctcgaacacg agcgctggct cgctgaacac ggcgacgaac
     6601 ggcagcgtca cgtcgacgtt ctccgctggc gtgtggacgg catcgggcgc gatcgcgaac
     6661 gtcaacgccc tcctcgcggc gctcacgttc acgccggcga cgaacttcag cggcaatttc
     6721 acgatcgcga cgagcgtcac cgacggtacg gcctcgccga ttgtcggcag caaggcgatg
     6781 accggtacgg cggtcaacga cgcgccgagc gcgacgaacc tgtttgcgca ggagacctac
     6841 ctcgaggaca cggccctcga cctcatcgac atcgtggtca gcgatccgga cactgcgaac
     6901 gtcaccgtta cgatgacgct ctcgaaccct gcggccggta gcctcaacac cggtacgtcc
     6961 ggcacggtga cctcgactta cgtcgccggc accggcgtgt ggacggcggc gggaccgatc
     7021 gcgaacgtca acacgctgct cgctgcgctg acgttcacgc cagcggcaaa ctcgatcgtg
     7081 tcgttcaacc tcgacgtgag catcacggat ggcgttgcgg cagccgtgac cggcgtgaag
     7141 ctcttcaccg gcgccgcggt caacgatccg ccggtggcga cgaacctgtc cgtcaacgag
     7201 acgtacgtcg agaactcgac gaaggacctc gtcgacatcg tcgtcagcga cgtcgacagc
     7261 gtcacggtca ccgcgacact cacgatctcg aaccccgcgg caggcgcgct gacgacgggc
     7321 agctccggct ccgcgacgtc gacctacaac tcgacgaccg gcgtatggac ggtgaccggc
     7381 ctcgtcttca acgtgaactc gctgctctcg acggtcgcct tcattcctgc ggcgaacttc
     7441 aacgcgacct tcacgatcgc aacggcgatc agcgacggcc cgaacacggt cagcggcagc
     7501 aagacgatga acggcgtcgc ggtcaacacg ccgccgagcg cgacgaatct gtccgccgcc
     7561 gagacgtaca cggaagacac gacgctcgac ctcgtcaaca tcgtggtcac ggacgctgat
     7621 agcgcgagcg tcacggcgac gctgacgctg tcgaacaccg cggccggcgc gctcagcacc
     7681 gcaacctccg gcagcacgac gtcggcatat accgctggca cgggcgtgtg gactgcatcg
     7741 ggcccgctcg ctgaggtcaa cgcattgctc gccggcgtca cgtttgtccc tacggcgaac
     7801 ttcaacgcgg cgttcaccgt cgcgacgaac gtcaccgatg gcggctcgac ggcagtcact
     7861 ggcacgaagg cgttcacggg cgttccggtc aacgatgcac cgaccgcgac gaatctctcg
     7921 gccgccgagg cctataccga agacacgccg ctgaacctca cggacatcgt ggtcacggac
     7981 gtcgacggtg ggaacatcac ggcgacgctg acgctctcca gcccgacggc cggcgcgctc
     8041 aacaccggca cctcgggcgc agtcacgtcg acgtacaacg cgggcaccgg cgtgtggagt
     8101 gcgtccggcg cgctcgcaaa cgtcaacgcg ctgctcgcgg gcctcacgtt cacgccagcc
     8161 gcgaacttca acggcaactt cacggtcgcg acgagcgtta ccgacaccgt cgctgcggcg
     8221 ttgaccggca cgaaggcgtt cacgggcacc gcggtgaacg atgcgcccgt tgccgtcgat
     8281 gacgcgggca ccgtgcccga gggcgcgcag atcgcaatcg acgtcagcgg taacgacacc
     8341 gacgccgacg acggagttga tccggcgacc gtcgcgttcg tcacgcagcc gacgcacggc
     8401 acgatcacgg caggctcgaa cggttcggtc gcgtatcacc acgatggcac cgatacgacg
     8461 agcgacacgt tcacgtacac gatcaaggat gcgagcggcg tcgtctcgaa tgctgcgacg
     8521 gtgacgctca ccatcactcc ggttgccgac gacgaggacg ctgacggcat ctccgacgat
     8581 gtcgacaact gcccggacgt gtcgaacgcg acgcagatcg atacagacgg cgacggcatc
     8641 ggcgacaagt gcgactcgac gcccggcggt gtggacagcg gctgtggctg tagcggtggt
     8701 ggcaacccgg ctggcagctg gccgctcctc ggagcgatcg gcctgctgtt ccgtcgccgt
     8761 cgtcgccgtt aacgcgaacc gagcgcggcg agcaccgcga tgtcgacgat atcggccgct
     8821 gcaaacagcg actcggcatc gagcggcatc gtgtagctcg gcacggcggc gccgacttcg
     8881 tcgcacatcg acttggcgaa tgcgtcgaag ctcggctcgc atccggcgag cgtcgccatc
     8941 acgccgatga tggcgttgac gtcgtcgagc ggctgcgact cgccgatcac gccgagcccg
     9001 gcgatcatca ccgtcggtgc tgcaccgtca tcgagcacga cgaagcgtgg cgcgataccg
     9061 ccgtggctgc cgccgccgat gtgaatcgcg gccgcgccgc gagcgagccg cttgagcaca
     9121 cgcagcgcct cggtggccgg aaggtccggc ggggagtccg caagcgacgc accggagggc
     9181 gcttcgaaga ccgcgactcg cgcaccgcga tcgagcgaca gcgcgcgctg cacgaatggg
     9241 ctctgcgcac gcccgagcat gcgggctctc tcgacggcgc gcagcgcatc atccgtctga
     9301 tcgaagcgct cgatgatgac cgaccgatcg agcaccgtgt cgacggcgcg cgacagctgg
     9361 ctgatagtcg tcacgccgag ctgcgtctcg aactgatagc gcggcttcgc ttcgccttct
     9421 tcggcgagct gcgcgatcga gtgcggtgcg ctcgatcggc gagggcgtgg gccgacgcgt
     9481 ggctggccat gcaggtcgag cgtctccagc tggtggcgca aatcggcgag cgtctgcgtg
     9541 cgctcctgcg gattcttcac gagcagcgcg gcgagcatgc catcccacgc ttgcgggata
     9601 ccttccgcga cgctcgacgc gaccggcggt tcttcgccga gatgctgcgc gacgaagtcg
     9661 ggaccgagga acggcagcct ccccgtgatc acttcgaaca gcgtcacgcc gagcgcgtag
     9721 aggtctgccg agatcgagat cgaggcgccc gtgatctgct cgggcgacat gtacgcgagc
     9781 gtgccgatga ggccgcccgt ctgcgtctgt cctagatcga cgaggtgcgc gaccccgaaa
     9841 tcgccgagct tcgcggtgcc ccgcgcgtcg aagaaaatgt tcgccggctt cacgtcgcgg
     9901 tgaacgacgc cgcggtgatg cgccgcttcg aggccaccga tgatatcgaa ggccatccgc
     9961 cggacctgcg gtgccgtcag ccgctcaccc gccccgagcc gcgctgccag cgagccaccc
    10021 ggcaggtact ccatcacgag gaagccgcgc tcgacggaga cgtcgtacac ctcgacgagc
    10081 gagggatggc gcagcgtgct cgcaagccgc gcttcgcgga cgaaccgctc gtacgccgca
    10141 ccgcctcgcg cgcccgatgc gaagaacatc ttgatcgcga cttgccgccc agcaacctcg
    10201 tcggtcgcga ggaacacgcg gcccgatgcg ccggatccga gcagcttctc gagtcggtag
    10261 cgcccggcga tcacttcgcg gtcgcgactc gtcttgcgct ccgtcgattc gtcccgccat
    10321 gcgcgcaggt acgtatcgag atccgcgggc acggattggt cgaccgcgcg cagctcgagc
    10381 agcgcatctc gcgctccgtc gcgcaggccc atcgccgcaa gcgtcgcgat caaatgccgc
    10441 tgcgcatcgg cgcggagctc cggctgcttg cgcgattctt gaagaagccg cgcagcctcg
    10501 ggataagcgc cacgccgcgc gagaatccgg ccgagcgcga gctggagcgg ggccttctcg
    10561 ctaggcgaag cgaggtcgag cgcgcgctcg atcagacgtc ccgcatcgcg gtcgcgacct
    10621 tggctctcga ggagccgcgc cgcttcgagc tccttgcgcg cacgcgtgaa caggtcgatc
    10681 gccttgttca cgtcgccgag cttctcggca atcggcgccg cctcggcgtg acgccgcatc
    10741 cgcacgagca tgtcgagcgc agccttgctg ccttcgggcg tcgcgttcag ctcgtcgagg
    10801 aggcccttcg ccgtcgcgtc gtcgtggagc tcgagcgcgt agcggagcgc ccgtggcaga
    10861 tcacccgccc cgcgcgcagc gtcgagcgcc gaccgaaaat cccacaactt ctcgtacagg
    10921 tcggccgcaa gcgcgtgatc accctccgcc tgcgcccgcc gcgcggcctc caaatgctga
    10981 ccggacgcga tcagctgttg gatctcggaa ttgatcgcca agcaccggca atggtacccc
    11041 accagggctc atgcaccagc gcagcgctca tcgctccctc ctcgtcgtcg gatccgtcgc
    11101 cttcgacgac atcgacggcc cgttcggtct ccacaaagac ttgctcggcg gctcggcctc
    11161 gttcatcacg cgcgccgccg cgtacttcac gcgcaacgtg tcggtcgtcg cggtcgtcgg
    11221 cgaggacttc ccgcagaagt acgtcgacga gatgtcccag cagggaatcg acgtctcggg
    11281 tatcgagcgc acgcccggcg agacgttcca ctgggtcggc aagtactcgc cggacctgtc
    11341 ctcgcgcgag acgatcgaca cgcgcctcgg cgtgtttgcg acgttccagc ccaagctcct
    11401 cgacgcgcac aaagatgcgc agctcgtcct cctcggcaat atcgatcccg agctgcagct
    11461 cgacgtcctc cgccaggtgc gcgcacccgc gctggtcgcg ctcgacacga tgaacctctg
    11521 gatcaacatc aagcgcgccg agctcgagaa gacgctctcg aaggtccaca cgctgatgat
    11581 caacgacgag gaagcgcggc tcctcgcggg cgaccacaac ctccgcaagg cggcggctcg
    11641 catcctcaag atgggcccga cgtccgtcat catcaagcgc ggcgatgcag gcgccctgct
    11701 cttccacggc ggcggcgtgt tcgcggcccc ggcgatgcaa ctcgaagacg tcaaagatcc
    11761 gacgggcgct ggcgacagct tcgcgggcgg cctgatgggc tacctcgcgt acgccggtga
    11821 cctctctccg aaatcgatcc gcaccgcgat gatcatgggc agcgtcatgg gcagcttcgc
    11881 cgtcgagaag ttctcggtcg acggtctgcg cgacctcacc gccgccaaga tccagcagcg
    11941 cttcgacgag atggccgagc tcgtccactt cgaaaaagta cgcctctaaa ccgatttgtt
    12001 tgcgacaaat gcctcggggc gactctcgag cacatgaggc ttcgctggct cgtcgtcgct
    12061 gcgtccgtcg cggcgtgctc gcgcgagcca gccgccgaga cggggccatc gttccgccgc
    12121 gacatcgcgc ctgtgctcga gaagcactgc accgcaaagg aatgtcacgg cgccgagccc
    12181 gcgccggatg ttgacctcga tctgcgagca ccgttcgcgt atcgccagct cgtcggcgtt
    12241 cctgccgaga tgggcgagat ccggcgcagc cgcgtggtgc cgtacgatgt ctacggtagc
    12301 tttctggtgc acaagctcgc tggaccgtcg gggccgcgcg aagggaagcg catgccgctc
    12361 gatccagcca cgggcgagcc gctcgagcga agcccgctgc cgtcgcgctt cgtcgacgat
    12421 gtgatcgtga agtggatcga agagggcgca cccgacaatt gatcagcggc tgcggcggtt
    12481 cacgtctcgt ttgcgcgcga ggacgaaccc ggcgagcgcg atgccgataa gcagcgctgc
    12541 aagcgccgcg agcgtttgac gggcatgcgg aaacaaaccg gagagcccaa cattcgtttg
    12601 ccgcgcgagc acgagctgcg cgattgcgct gtcctgtccg cgtgtgcgaa gtggcgtgcg
    12661 ctcggcgagc cacgtgccgt cgtcgaccga gagctccgag attccgcgat cgtggaccgc
    12721 gagtagcacc gacttcggaa cttggccttc ggcaataccc tcggccgtga cgaccgagag
    12781 cagcgtgccc tgcccgacgc cgacctcgtc gagcaagcgt ccggcctcca cgagctgcat
    12841 gaacatcgag ctcggaacgg cgccgactcg cttcgcgcgc gcaaacacga cgtacagctt
    12901 tcgttgcggt acgccaccga gcaggccgga gcgtacgact gctggatccg caccgtcgat
    12961 caccccgagg tacgtctcgg tctgcgacga gtacgccgcg gcaagcggcg cgatctcggt
    13021 gacgctgctc ccgaagagct cggggctgac gctgtcgtac aacaggcgtc ccttgtgatc
    13081 ggcgatcgct aggacgtcac cgtgtcgcga ccggacgagg ccgacccagt ctgcgctctg
    13141 gagcgtttcg tgctggcgcg cgatacgtgc tcggtcttct gcctcgtcgc cgagaccgaa
    13201 gtccgattgg tcgcgcgcgg tcgcgatctc gagcatgatc ggaagtcggt acatcgcatc
    13261 ggcctgggcg atgaatgcgt ccgtacgcag agcgaacagc ttgtcgacct gcgcgcgggc
    13321 ggcctcgaaa cttccgcgcg tcacggcgtg cgtccgctct cgaagcacgt agtcggcacc
    13381 aaacccgacg gcgacgagcg cgaacaacac gagcgcaccg aatgcgacgc gccgaggctg
    13441 gcgcccacga tcacgggcga gcgcgtgaag caggtcctcc atggaggcga agcgatcgcc
    13501 cggcttcgtc gacagcgcgc gctgcaggat cgcgcggagc gacgcgtgga cgtgcgttcc
    13561 tgccggcggc ggctcgacct tgccttcgag gatcgacgtg gcgagctcgt cgcgcgactt
    13621 gcccgcgaat ggccgcgagc cgtagagcgc ttcgtacagg gcgacggcga agctgaactg
    13681 atcgctgcgc ggatcgacgt tcgtccccgc gtgttgctcg ggcgccatat acgcgggggt
    13741 gccgacgatc ggccgcgtca tcccttctgc actcgtgtcg acgctttccc cgtccgcttg
    13801 atcgaacgcg aggccaaagt ccgtgacgcg cgcgcgcccc gccgcgtcga cgagcacgtt
    13861 gtcgggcttg aagtcgcggt gaaccattcc tgcgttatgc gcagcgatga gtccatggcc
    13921 cgcgccgatg aagacttcga ggatctcggc ggtcgtccgc gacgaggtca gccactcacg
    13981 caggctctga ccggagatcc gctccatccc gatgaagacc tggccttcat gcgtgccgac
    14041 gtcgtagacg gacaccacgt tgggatgcga gacgcgagcc atcgcctgtg cctcacggac
    14101 gatgcgacgc gtggcatcgg gattcgcagc ggagaactcg gggcgcatga ccttgatggc
    14161 aatgtctcga tgcagatccg gatcgtgtgc tgcgtagaca acccccatgc cgccgaggcc
    14221 gagcgtgtcg ccgatgatgt agcgaccgac cttcgtgccc ttcggaaggc tcgagcgcgg
    14281 cccttgaggc atcgaggtcg agacctccgt cggttgaaat gcctcgggtg ggtgcgttcc
    14341 gagcgttggc tcggatccgc gacgttcgac aaccgctgcg cgcaccaaca tcgcgacggt
    14401 cgagcggcag tcgtcgcatg tgtcgagatg ctcttcgagc gcagcgcgtt cctcggcagc
    14461 gagcgaatcg tcgacgaatc gcgccagcgc gtcagaagat gcgtggtcgc tcacgcgatg
    14521 acgccttgtg ggatactcgc gtcatgagca acctcgccgc cgtctttctc gacgcgcgtg
    14581 gcgagactgc atctgatgcg gaccgaggcg cgctcgaggg tgcgctgtcc gacgcggtca
    14641 ctgcggcgac gagcggatgg cctaccgtgc gagtggatcc tgcagcatgg attcgcgcgc
    14701 tggccgagct cgcgccgaag ccgctggatc tcgcgaagcc gctcggcgag gtgctcgtcg
    14761 ccgaccacta tctggcgttc gcgtgcgcag ccggggatcc cgcagccgtc gccgagtgca
    14821 acgcgattct cgtacgcgag gccgggttcg ccgccgacgg cacgaagatg cacgcgtcgg
    14881 tccgcgacga ggccacacag atcgtgcgcg ctacgctgct cgcaccgcgc gaaggcaagc
    14941 cgcctgccat cggggattac gcgggccgcg gcgcgctgcg cagctggctt cgcgtgtgcg
    15001 tctcgcgaga gcttgtccgg atcgcgaagg cgcagcagcg catcgagccc ctcgaagagc
    15061 acctcgtcgc tgatcccgga tacggcgacg accctgcgct cgaagagctc aaggcgaagt
    15121 atcgcgcaca gctcgcggac gcgtttcgca cggcactgac ggagctgccc gctcgcgagc
    15181 gcacgctgct tcgctatcag ctgatcgacg gactcacgat cgacgagatc ggcggcatct
    15241 ttcgcgttca ccgcgcgacc gcggcccgct ggatcgcgaa gatccgcgac gatctcgtaa
    15301 tgcgcacgcg cgagatgatg gcggcgtcgc tcggcgtcga tacgtccgag gccgcgagca
    15361 tcgtgcgcct cgttcagagc cagctcgacg tcagcgtcat tcgccatctc ggtccgccgc
    15421 cgaagaaacg ctgaccgcat cacgagcccg gagcatagac cgtcgtcggt cgatacttct
    15481 caccgtactt gttcggatgc gtgaagtcct tcttcacctc gtcgagcttc agcgtgacct
    15541 cggtgacacc gtcggctgcg acgctgatct tctggctgac cggctgcaca gcacgacgtg
    15601 agaacgcgaa cagcgtccac gtgcccggcg gtacgtgatc gatccggaag ct
//
LOCUS       DATLBZ010000477         9371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10745058, whole genome shotgun sequence.
ACCESSION   DATLBZ010000477 DATLBZ010000000
VERSION     DATLBZ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10745058"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VMZ53_24190"
     CDS             complement(<1..1333)
                     /locus_tag="VMZ53_24190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31635.1"
                     /translation="MLWRLMFAAYAALGIFLAVAYTQYPDIKPQYQLYVYGAFTVGLA
                     VMALQYAFILRKPFLSMRQLHSLDTFYVIASLSIIAFCAVVSFDRRQAAYSCLIYSCF
                     AVLTRALIVPSTGRRTAIVTSIGYIPMSLGAIFLSKWAEWPPGKEVPWVAFLIGYGQI
                     VGVAILLSAAGSQLIYGLRRKVSAAQQLGKYTIVKKIGAGGMGVVYLAHHLLLRRPTA
                     VKVLQPDRVGAENLERFEREVQHMSQLTHPNTVAVYDYGHSSDGVFYYAMEYLGGGID
                     LQNLVAKHGKQPAERVRQILMQTCGALQEAHDRGIIHRDIKPANIILCERGELPDVVK
                     VVDFGLVKELTADTGQSQAMIMGTPGYVAPEVVTDPDAIGPSVDLYALGAVAYYLLTG
                     RRVFDGKTVLDVCLQHVTKEPTPPSQIAATHVPRVLEALIMQCLAKTPAQRP"
     gene            1636..2643
                     /locus_tag="VMZ53_24195"
     CDS             1636..2643
                     /locus_tag="VMZ53_24195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31636.1"
                     /translation="MKAVISLGLALAASQTAFAQPVTSPATCAVKVMRAPEDVRQVVE
                     AWVQAEPRCSVALDVRIIPTEGGLYLFALDERGRIRERIVPDAQSAGVLVASWVADDT
                     MYTPPPKQPTAAADERVDPFTNRGGGPNSDLTTPGMTLSNSEPVDGGEVPTVRKNVSK
                     WVGAGFLIDKTSEAGGLRVDADVWTRGTYSLGAALSSTTNHMDVIAQQGAGWMQARDI
                     KVVATGSHTWQSGRWFVRGAAGVGAVHTEVLVTVDQPSFDGTYGYYRASGWTGVAEAS
                     ALGGAELGKNWSLSFGPMFTVYAQDLHTYDESGYMTYEALELSRRDIEVLFYTGLKYR
                     L"
     gene            2640..3215
                     /locus_tag="VMZ53_24200"
     CDS             2640..3215
                     /locus_tag="VMZ53_24200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS31637.1"
                     /translation="MIKKGQLLQLRPEARVDEMSDAGLVAACAAADRTARALLFERHV
                     DAIHRFVGRMKGSDADAIDDLVQMTFVRAFQSAGRFRGTSARSWLYGIAANVVREHAR
                     KEIRRKRALSVVADELPRATRGHDVMLAKLPAAIDSLPHELRAAFVLIDMEGERGIDA
                     AAALGIPEGTLWRRVFNARTALRKFLAGGGA"
     gene            3215..4042
                     /locus_tag="VMZ53_24205"
     CDS             3215..4042
                     /locus_tag="VMZ53_24205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31638.1"
                     /translation="MSPDEIAAHEPEIVELAKRLPWKPLSEEHRRRLAAEVMAQADVA
                     VVRSRRRGWAIAAVVAAAAAVALVVGLRRDVATHAPTIAAGESPESVETSGRSTSALE
                     SPPAPAVAPEIHGVVQLHDGELTIDTRQREPVTVVTGGTRVTITSSRATVVARRGVII
                     TAQVFAGTAEITEAGHTRIVEAGEVWTPSPAATSLQSFRAGYELLRDGKYDDAVRVLD
                     LATDPVVLEDATFWAAIACERAGNKAEAARRLEAFMTAFPASPRLDDARSALERVTR"
     gene            4085..6112
                     /locus_tag="VMZ53_24210"
     CDS             4085..6112
                     /locus_tag="VMZ53_24210"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HUS31639.1"
                     /translation="MKAACPSCGAEIEFRYDDSFVRVCEYCRAAVQRTDRGLDSLGKF
                     ADLVPMDSPLRLFSDGQMGSQTFILVGMAQLRQEAGGITQEWYAKFSNTWGWLAEAQG
                     RYYLTFEVEGAQLPPLGSLQAGAKVQLPTMLGAMREFTVAEVSSATYVAANGELPFRL
                     APGTTYRYADLSDGQGNFATIDYGEGSDAPTLYTGQQLSLQDLNISGGEVGPSTAKKV
                     GASRLGCPNCNAPVDVFAAGQSQRAVCGHCNTLLDIQSGTGQVIEKLKTKAAPRIPLG
                     SSGTFPEGKLRVIGYVQRSANIEGVWYPFDEYLLYEPSIGFRWLVQSDGHWSYVQPVD
                     IGAIDYAGAKVKYDGVKFRMFQRSELRVDCVLGEFYWQVKVGETTFGEDYIAPPAMLS
                     KEATNKEENWSLSTYMKTAQIAAAFGDRADVEVGRTIGVGANQPDPAASATTPLAIAF
                     LAMIILGIVFASKAEKVERMSQTVSIPAGVILPTPEGTPPDPNAPNIFFSQPFDLAAD
                     QNIEVGFNASLNNNWAYVIASLVNTSTGDLVTLDGSMEYYSGVDGGESWSEGKPTDVE
                     VVGPMPAGQYVLRLETQQGGTVGDVPLFIRVRQGVFRFRYWALGMGVLSIPLFIFGLI
                     SYSHEKRRWSNSTEESGPPKTPLVLLVMGIFGIFWLLWALLRVFASSNNDD"
     gene            6147..6353
                     /locus_tag="VMZ53_24215"
     CDS             6147..6353
                     /locus_tag="VMZ53_24215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31640.1"
                     /translation="MPRPRQGKVLFVFVTALCVAIVGGYVWFASTQRELGSEERDQIP
                     SGMRSTPGGYRTYSYWHSGYHGGK"
     gene            6353..6574
                     /locus_tag="VMZ53_24220"
     CDS             6353..6574
                     /locus_tag="VMZ53_24220"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HUS31641.1"
                     /translation="MKLDNIGEAIVSSSVFAGIGLVVFGLCFWVMNKLAPFSVKKEIE
                     EDQNTALAIIMAGVIIGVSLIISSAIQGG"
     gene            6624..8108
                     /locus_tag="VMZ53_24225"
     CDS             6624..8108
                     /locus_tag="VMZ53_24225"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HUS31642.1"
                     /translation="MFVVAICGLVYELLAGTLGSYLLGDSVTQFSLVIGLYLSAMGVG
                     AWLSRRLDADLARRFLDIELAVAVVGGLSAPILFVAFGSLEHFQIVLLAFVFAIGVLV
                     GLELPLLMRLLEGQVAFKDIVSRVLTFDYIGALAAALLFPLFLVPKLGLVRSSLVMGT
                     ANAGVALWGTWLLAGRLGRKGVVAVRVRAVIVLLGMIVAFAIADKFTTWAEDEMYIDP
                     IVYSRQTQYQRIIVTQGKSGFQLFLNGNLQFSSADEYRYHESLVHPPMLAADGPPRRV
                     LILGGGDGLALREVLAHPSVEAVTLVDLDPAMPQLAMSFPPLVALNKNSFRDHRVTLV
                     NDDADVWLQEHPSQWDVIIVDFPDPNSFALGKLYTRAFYRRVLAHLSPGGAVSIQATS
                     PLFARKSYWCIVDTVEAAGFAVRPYQVAVPSFGVWGYVLAKRTDFKAPEHAPSAVALR
                     YLNDDALRGMFVMPADMEHVDVEINQLDNQALVRYYEREWRKWE"
     gene            8099..>9371
                     /locus_tag="VMZ53_24230"
     CDS             8099..>9371
                     /locus_tag="VMZ53_24230"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS31643.1"
                     /translation="MGVTRRDFVAMMLGAPFAHACSRGVTSRALPPGSLIDTGMARAH
                     AAVRDGTIPTPTQWRKQPVVIVGGGVAGLSAAWELRRRGVKDVLLLELDDVVGGTARG
                     GSSSVTPYPWGAHYIVAPQKEQGDLTALLTEMKAIEGYAADGTPIVDETLRCREPEER
                     VWYLGRWWEGLYMEAASSKEDRRQLAAFNAEIDRWSAWRDEQGRRAFAIPRSTASDAV
                     EVMELDRLSFAQWLDARGLTSERLRWLCDYACRDDYALTAAQTSAWAGLFYFAGRQSA
                     PGAEAQSVVTWPDGNNALITHLAVGATIERGIAVVDIDEVSGRLIGVGPAGPIGIEAE
                     KIIVATPTFVANRLVRARRPVGVPHVVPDAATHRKSNVGADYGAWVVANVHLRARPIE
                     RGRGAPPAWDNVFRDSPSLGYVSATHQRGRDR"
BASE COUNT         1545 a         2971 c         3230 g         1625 t
ORIGIN      
        1 cgggccgctg cgccggcgtc tttgcgaggc actgcatgat gagcgcctcg agcacgcgcg
       61 ggacatgggt cgctgcgatc tgcgacggtg gcgtcggctc cttcgtcaca tgctgcaagc
      121 agacgtcgag cacggtcttg ccgtcgaaca cgcggcggcc cgtgaggaga taataggcga
      181 cggcgccgag cgcgtagaga tccaccgacg ggccgatcgc atctggatcg gtgacgacct
      241 ccggcgcgac gtagcctggc gtgcccatga tcatcgcttg cgactggccg gtgtcggcgg
      301 tgagctcctt cacgaggccg aagtcgacga ccttcacgac gtcgggcagc tcgccgcgtt
      361 cgcacaggat gatgttcgcc ggcttgatgt cgcgatgaat gatgccgcgg tcgtgcgctt
      421 cttggagcgc gccgcacgtc tgcatcaaga tctgccgcac gcgctctgcc ggctgcttgc
      481 cgtgcttcgc gacgaggttc tgcagatcga tgccgccgcc gagatactcc atcgcgtagt
      541 agaagacgcc gtccgagctg tgcccgtagt cgtagaccgc gacggtgttc gggtgcgtta
      601 gctggctcat gtgctgcacc tcgcgctcga agcgctcgag gttctcggcg ccgacgcgat
      661 cgggctgcag gaccttgacc gccgtcggac ggcgcagcag caggtgatgc gcgagataca
      721 cgacgcccat tccgcccgcg ccgatcttct tcacgatcgt gtacttgccg agctgctgcg
      781 cggcgctgac cttgcggcgc agtccataga tgagctgtga ccccgcggca gagagcagga
      841 tcgcgacgcc cacgatttgg ccatagccga tgagaaaggc gacccacggc acttccttgc
      901 ccggcggcca ctcagcccac ttcgagagga agatggcgcc cagcgacatc gggatgtagc
      961 cgatcgacgt cacgatcgcg gtgcggcggc cggtgctcgg cacgatcagg gcgcgcgtga
     1021 gcaccgcgaa gcacgagtag atgaggcacg agtatgccgc ttgccgccga tcgaacgaga
     1081 cgaccgcaca gaacgcgatg atcgagagcg acgcgatcac atagaacgtg tcgagcgaat
     1141 gcagttgccg catcgagaga aacggcttgc gcaatatgaa cgcgtattgc agcgccatga
     1201 cagcgaggcc gactgtgaag gctccgtaca catagagctg gtactgcggc ttgatgtccg
     1261 ggtactgcgt gtatgcgacc gcgaggaaga tgccgagcgc cgcatacgcc gcgaacatga
     1321 ggcgccacag cacgatcaag cgtgcgtgaa ggtaggcgcg cgactcttcc tcggtgctcc
     1381 cgctggtcca cggaccatgg atcatccgac gcgagctggc tgcgtctgcc actcccacca
     1441 cgttgccggc cagcatgcca gagcgcgtta gcgtccacgt ggatttaacc gggcagagca
     1501 gtgaaaaaat cgggatcacc cgtgatgaat tacgacccct gcggcaacca agcaggggaa
     1561 ggccgacggc ggctgcaccg aggtacaaaa cgtgcggtct tcggtgcagt cgccggtcgg
     1621 ttacgtagga ggcacatgaa agcggtcatc tctctcggtc tagctctggc ggcgtcacag
     1681 acggcgttcg cccaacccgt gacgagcccg gcgacctgcg cggtgaaggt gatgcgtgct
     1741 cccgaggacg tccgtcaggt cgtcgaggcg tgggtgcaag cagagcccag atgcagcgtc
     1801 gcgctcgacg tgcggatcat tccgaccgag ggcggcctct acttgttcgc gctcgacgag
     1861 cgtgggcgga tccgcgagcg catcgttccg gacgcgcaga gcgcaggcgt actggtcgcg
     1921 agctgggtcg ccgacgacac gatgtacacg ccgccaccga agcagccgac ggccgcggct
     1981 gacgagcgcg tcgatccatt cacgaaccgc ggaggcggac cgaattcgga tctgacgacg
     2041 ccgggtatga cgctgagcaa cagcgagccc gtcgatggcg gcgaggtgcc gacggttcgc
     2101 aagaacgtct cgaagtgggt cggcgcgggc tttctgatcg acaagaccag cgaggctggt
     2161 ggcctccgcg tcgatgcgga cgtctggacg cgcggcacgt attcgctcgg cgcggcactg
     2221 tcgtcgacga cgaatcatat ggacgtcatc gcccagcagg gcgcgggctg gatgcaggcg
     2281 cgcgacatca aggtcgtcgc gaccggctcg catacctggc aatcggggcg gtggttcgtg
     2341 cgcggagccg cgggtgtcgg cgcggtgcac acggaggtgc tcgtcacggt cgatcaaccg
     2401 agcttcgacg gcacgtacgg ctactaccga gcaagtggct ggacgggcgt tgcggaggca
     2461 tctgcgcttg gcggcgcgga gctcggcaag aactggtcgc tcagcttcgg tccgatgttc
     2521 accgtctacg cgcaggattt gcacacctac gacgagagcg gctacatgac gtacgaggcg
     2581 ctcgagctgt cgcgccgcga tatcgaggtc ctcttctaca cggggctcaa gtaccgcctg
     2641 tgatcaagaa gggtcagctg ctccagctcc gcccggaggc gcgcgtcgac gagatgtcgg
     2701 acgccggcct tgtcgcggcg tgtgcagcag ctgatcgtac ggcacgcgcg ctgctgttcg
     2761 agcgccacgt cgatgcgatt catcggttcg tcgggcgcat gaagggcagc gacgccgacg
     2821 cgatcgatga cctcgtgcag atgacgttcg tgcgggcgtt tcagtcggcc ggtcgctttc
     2881 gcggcacgag cgcgaggtcg tggctctacg gcatcgccgc gaatgtcgtg cgcgagcatg
     2941 cgcgcaagga gatccggcgc aagcgagcac tcagcgtcgt cgccgacgag ctgccgcgcg
     3001 cgacgcgtgg gcacgacgtg atgctcgcga agctgccggc cgcgatcgat tcgttgccgc
     3061 acgagctgcg cgctgcgttc gtgttgatcg atatggaagg cgaacgcggc atcgatgcag
     3121 cggcggcgct cggcattccc gagggcacgc tgtggcgccg cgtgttcaat gcgcgcacgg
     3181 cgctgcggaa gtttctcgcg ggaggtggcg cgtgatgtcg cccgacgaga tcgctgcgca
     3241 cgagccggag atcgtcgagc tcgcgaagcg cttgccgtgg aagccgcttt cggaagagca
     3301 ccgccggcgg ctcgccgccg aggtcatggc gcaggccgat gtcgccgtcg tgagatcgcg
     3361 gcgtcgcggt tgggcgatcg cagcagtcgt tgcggctgca gcggccgtgg cgctcgtggt
     3421 cggactgcgt cgagatgtcg ccacgcatgc accgacgatc gcggcagggg agtcgcccga
     3481 gtctgtcgag acctcaggtc gctcgacgag cgcgctcgag tccccgccgg caccggccgt
     3541 ggccccggag atccacggcg tggtgcagct gcacgacggt gagctgacga tcgatacgcg
     3601 tcagcgcgag ccggttaccg tcgtcaccgg cggcacgcgc gtgacgatca cgagctcgcg
     3661 ggcaacagtg gtcgctcggc gtggcgtcat catcacggcg caagtcttcg cggggacggc
     3721 ggagatcacc gaggcgggcc acacgcggat cgtcgaggca ggcgaggtgt ggacgccgtc
     3781 gccggccgcg acgtcactgc agtcgtttcg cgcgggctac gagctgctgc gcgatggcaa
     3841 gtacgacgac gcggtccgcg tgctcgatct cgcgaccgac cccgttgtcc tcgaggacgc
     3901 gacgttttgg gcagcaatcg cgtgcgagcg cgcgggcaac aaggctgaag cggcgcgccg
     3961 gctcgaagcc ttcatgacgg catttccagc ctcgccgcgg ctagacgatg cgcgatcggc
     4021 tctcgagcgc gtgactcgct agtatttctg gtacgttcgc ggcgatgaac ggggtggtcc
     4081 ccgcatgaaa gccgcctgtc cgtcctgcgg cgcggagatc gagttccgtt acgacgactc
     4141 gttcgtgcgc gtctgcgagt actgccgcgc ggcggtgcag cgaaccgatc gcggcctcga
     4201 ctcgctcggc aagttcgccg acctcgtgcc gatggactcg ccgctgcgcc tgttctccga
     4261 cggccagatg ggcagccaga cattcatcct cgtcggcatg gcgcagctgc gccaggaggc
     4321 gggcggcatc acgcaggagt ggtacgcgaa gttctccaat acctggggtt ggctcgccga
     4381 agcgcaaggc cgctactacc tgacgttcga agtcgaaggc gcgcagctac cgccgctggg
     4441 ctcgctccag gccggcgcga aggtccagct gccgacgatg ctcggcgcga tgcgcgagtt
     4501 caccgttgcc gaggtgagca gcgcgacgta cgtggcggcg aacggggagc tgccgtttcg
     4561 gctcgcgccc gggacgacgt atcgctacgc cgatctcagc gacggccagg gcaacttcgc
     4621 gacgatcgac tacggcgagg gcagcgacgc accgacgctc tacaccggcc agcagctctc
     4681 gctgcaggac ctcaacatca gcggcggtga ggtcggcccg tcgacagcga agaaggtcgg
     4741 cgcatcgcgg ctcggctgcc cgaactgcaa tgcgcccgtc gatgtgttcg cggcgggcca
     4801 atcgcagcgc gccgtgtgcg gccactgcaa cacgctgctc gatatccagt ccggcacggg
     4861 ccaggtcatc gagaagctca agacgaaggc cgcgccgcgc attccgctcg gttcgagcgg
     4921 gacgtttccc gagggcaagc ttcgcgtcat cggctacgtc cagcgcagcg cgaacatcga
     4981 gggcgtctgg tatccgttcg acgaatacct gctgtacgaa ccgagcatcg gttttcgctg
     5041 gctcgtgcag tccgacggcc actggagcta cgtgcagccg gtcgacatcg gcgcgatcga
     5101 ctacgccggc gcgaaggtca aatacgacgg cgtgaagttc cggatgttcc agcgctccga
     5161 gctccgcgtc gattgcgtgc tcggcgaatt ctattggcag gtgaaggtcg gcgagacgac
     5221 gttcggcgag gactacatcg caccgccggc gatgctgtcg aaggaagcga cgaacaaaga
     5281 agagaactgg tcgctgtcga cgtacatgaa gactgcgcag atcgcggccg catttggcga
     5341 tcgcgcggac gtcgaggtcg gtcggacgat cggcgtcggg gccaatcagc ctgatcccgc
     5401 tgcgagcgct acgacgccgc tagcgatcgc gttcctcgcg atgatcatcc tcgggatcgt
     5461 gttcgcgtcg aaggccgaga aggtcgagcg tatgagccag acggtgagca tcccagcggg
     5521 tgtcatcttg ccgacgcccg aaggcacgcc gcccgacccg aacgcgccga acattttctt
     5581 ctcacagcca ttcgacctcg ccgcggatca gaacatcgag gtcggcttca acgcgtcgct
     5641 gaacaacaac tgggcgtacg tgatcgcgtc gctcgtcaac acatcgacgg gagacctcgt
     5701 cacgctcgat ggcagcatgg agtactactc cggcgtcgac ggtggcgagt catggtcgga
     5761 gggcaagccg accgacgtcg aggtcgtcgg gccgatgccg gcggggcagt acgtgcttcg
     5821 gctcgagacc cagcaaggcg gaacggtcgg cgacgtgccg ctgttcattc gcgtgcgcca
     5881 aggcgtgttc cgcttccgct actgggcgtt gggaatgggc gtgctctcga tcccgttgtt
     5941 cattttcggg ctcatcagct actcgcacga gaagcggcgc tggtcgaata gcaccgagga
     6001 gagcgggcca ccgaagacac cgctcgtcct gctcgtcatg ggcatcttcg gcatcttctg
     6061 gctcttgtgg gctctcctac gcgttttcgc gagcagcaac aatgacgact gaagaaccac
     6121 agaaacgtcc cgccgatcat ccgccggtgc cgcgcccccg gcagggcaaa gtcctcttcg
     6181 tcttcgtgac ggctctctgc gtcgcgattg tcggcggcta cgtgtggttt gcgtcgacgc
     6241 agcgcgagct cggttccgag gagcgcgatc agattccatc cggcatgcgg tcgacgccgg
     6301 gcgggtaccg tacctacagc tactggcaca gcggatacca cggaggtaag tgatgaagct
     6361 cgataacata ggtgaggcga tcgtctcgtc gtcggtgttc gccggcatcg gcctggtcgt
     6421 ctttggcctt tgcttctggg tcatgaacaa gctcgctcca ttctcggtga agaaggagat
     6481 cgaggaagat cagaacaccg cgctggccat catcatggcc ggagtgatca ttggcgtcag
     6541 cctcatcatc tcctccgcca tccaaggcgg ttgaaccgcc cggcggcgag ccgccgattc
     6601 gcgcgagcgc gttgtatctc aacgtgttcg tggtcgcgat ctgcggcctc gtctacgagc
     6661 tgctcgccgg cacgctcggt agttatctcc tcggcgattc ggtcacgcag ttctcgctcg
     6721 tcatcggtct ctacctctcg gcgatgggcg ttggggcgtg gctctcgcgc aggctcgatg
     6781 cagacctcgc gcggcgcttc ctcgacatcg agctcgcggt cgcggtggtg ggtggtctct
     6841 cggctccgat cttgttcgtc gcgttcggct cgctcgaaca cttccagatc gtgctgctcg
     6901 cgttcgtgtt cgcaatcggc gtgctcgtcg gcctcgagct gccgctgctc atgcgtttgc
     6961 tcgaagggca ggtcgcattc aaagacatcg tctcgcgggt cctgacgttc gattacatcg
     7021 gtgcgctcgc ggcagcgctg ctgttcccgc tgttcctcgt gccaaagctc gggctcgtgc
     7081 ggtcgtcctt ggtcatgggc accgcgaacg ccggcgtcgc gctctggggc acgtggctgc
     7141 tcgccgggcg actcgggcgc aagggcgtcg tcgccgtccg cgtgcgcgcg gtcatcgtgc
     7201 tgctcgggat gatcgtcgcg ttcgcgatcg ccgacaaatt cacgacgtgg gccgaggacg
     7261 agatgtacat cgatccgatc gtctacagca ggcagacgca gtaccagcgc atcatcgtca
     7321 cccagggcaa gagcggcttt cagctgttct tgaacggcaa cctgcagttc tcgtcggcgg
     7381 acgagtaccg ctatcacgag tcgctcgtgc acccgccgat gctcgccgcc gacggcccgc
     7441 cacgccgcgt gctgatcctc ggcggtggcg acgggctcgc gctccgcgag gtgctcgcac
     7501 atccgtcggt cgaagcggtg acgctcgtcg atctcgatcc ggcgatgccg cagcttgcga
     7561 tgagctttcc gccgctcgtc gcgctgaaca agaactcgtt tcgcgatcac cgcgtgacgc
     7621 tcgtcaacga tgatgccgac gtctggctgc aagagcaccc tagccagtgg gacgtcatca
     7681 tcgtcgactt cccagatccg aattcgttcg cgctcggcaa gctgtacacg cgcgcgttct
     7741 accgtcgagt gctcgcacat ctctcgccag gtggcgcggt ctcgatccaa gcgacgtcgc
     7801 cactgttcgc gcgcaagtcg tactggtgca tcgtcgatac ggtggaagcg gcgggcttcg
     7861 cagtgcggcc gtaccaagtc gcggtgccat cgttcggcgt gtggggctac gtgctcgcga
     7921 agcggacgga cttcaaggcg cccgagcacg ctcccagcgc cgtcgcgctg cgctacctca
     7981 acgacgacgc gctgcgcggc atgttcgtga tgcccgcgga catggagcac gtcgacgtcg
     8041 agatcaacca gctcgataat caagcgctcg ttcgctacta cgaacgcgag tggcgcaagt
     8101 gggagtaacc cgccgcgact tcgtcgcgat gatgctcggt gccccgttcg cgcatgcgtg
     8161 cagccgtggc gtgacgtcgc gagcgttgcc gcccggctcg ctgatcgata ccggaatggc
     8221 gcgtgcccat gcggcagtac gagacgggac catcccgact ccgacgcagt ggcgaaaaca
     8281 gcccgtcgtg atcgtcggtg gtggtgtggc ggggttgtcc gcggcgtggg agctgcgacg
     8341 tcgcggcgtg aaggacgtgc tgctgctcga gctcgacgat gtggtcggcg gcacggcgcg
     8401 cggcggctcg tcgtcggtga cgccgtatcc gtggggagcg cactacatcg tcgcgccgca
     8461 gaaagagcag ggcgatctga cggcgctgct caccgagatg aaggcgatcg aaggctacgc
     8521 ggcggacggc acgccgatcg tcgacgagac gctgcgctgt cgcgagcccg aagagcgcgt
     8581 ctggtatctc ggccgatggt gggaaggcct gtacatggag gcggcgtcgt cgaaggaaga
     8641 tcgtcgccag cttgccgcgt tcaatgcaga gatcgatcgg tggtcggcgt ggcgcgacga
     8701 gcagggccgg cgggcattcg cgatcccgcg ctcgacggcc tcggacgccg tagaggtcat
     8761 ggagctcgat cgcttgtcct tcgcgcagtg gctcgacgcg cgcggcctga cgtcggagcg
     8821 cttgcgctgg ctgtgcgact acgcgtgccg cgacgactac gcattgaccg ccgcgcaaac
     8881 gagcgcatgg gcgggcctgt tctacttcgc cggacgccaa tccgcgccgg gtgccgaagc
     8941 gcagtccgtc gtcacgtggc ccgacggcaa caacgcgctg atcacgcacc tcgcggtggg
     9001 cgcgacgatc gagcgtggca tcgcggtcgt cgacatagac gaggtgagcg gacgcctgat
     9061 cggcgtggga ccggcgggac ctatcgggat cgaggcggag aagatcattg tcgcgacgcc
     9121 tacgttcgtg gcgaatcggc tcgtgcgagc tcgtcgtccg gtcggcgtgc cgcatgtcgt
     9181 gccggatgct gcgacgcatc gcaagagcaa cgtcggcgct gactacggtg cgtgggtcgt
     9241 cgcgaacgtg cacttgcgcg cacgcccgat cgagcgcggc cgcggtgcgc cgccggcatg
     9301 ggacaacgtg ttccgcgata gcccgtcgct cggctacgtc tccgcgactc atcagcgcgg
     9361 ccgcgatcgc g
//
LOCUS       DATLBZ010000478        17491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9553163, whole genome shotgun sequence.
ACCESSION   DATLBZ010000478 DATLBZ010000000
VERSION     DATLBZ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17491
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9553163"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..980
                     /gene="dapF"
                     /locus_tag="VMZ53_24235"
     CDS             90..980
                     /gene="dapF"
                     /locus_tag="VMZ53_24235"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HUS31644.1"
                     /translation="MRFAKYQGLGNDFLVVDLRDVRGAEADAAQNPDVVKALCDRQFG
                     VGGDGVLAVLPGDAGADARMRVLNSDGSEAEMCGNGIRCVAKELYDRGGVKKDRIAID
                     TGAGRLVCAISEKGGVAETVTVEMGAPRLLRGEIPMTGPANERCIEQPLSLSADSTEL
                     QITAVSMGNPHAVAFVAGKQMQLATTIGPRVENHAWFPKRTNAEFARVISPTEIELVV
                     WERGCGITLACGTGACATVVAAVVTGRAQEGSDVLVHLPGGDLTITVLPNFANVLMKG
                     PARHVFDGELDPRRVTPRPA"
     gene            complement(1132..1932)
                     /locus_tag="VMZ53_24240"
     CDS             complement(1132..1932)
                     /locus_tag="VMZ53_24240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31645.1"
                     /translation="MRFGNVARVIAVSAACLAAVGSATSNSSNPPATQAQGANANMSP
                     PPPREMEPARALGLWRSTFGAVKIEADNSRGGVQSGAIQGVWVYQRQGQEVVGYFNGS
                     LRGNVFNFRWQEPNNPPLTGEGYLVFDPSGRQYSGRWWSDKRDRVGDWNGWRQAVSSQ
                     PAPQYGGQPYGGQNPYMNSQYGGQTYGRPPQPQQPQQPQYPQQYPQQYPQQPQQPTQP
                     YYPQQPQPQQPNPYGPQQPSPYGPQQPTQPYYPQQPAPQPQGPYYPPR"
     gene            2037..2110
                     /locus_tag="VMZ53_24245"
     tRNA            2037..2110
                     /locus_tag="VMZ53_24245"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2071..2073,aa:Ile,seq:cat)
     gene            2166..2936
                     /locus_tag="VMZ53_24250"
     CDS             2166..2936
                     /locus_tag="VMZ53_24250"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HUS31646.1"
                     /translation="MRDQLVYLLELQNFDGKVRELDAAAKQLPAKLDPLRRDLAKLQG
                     MLDAERAKMAETQNWRKSQQELIDREREALKSAQSKFQASKNTKEFSAASREVENKRK
                     SIQDREGELKKVTEATNAQETALSARDADVQKLRDELAAGEAAMADQVKSVGDQLAEA
                     KAARDAARSKVEKKWLSTYDSLAAKRGYAVAPVIKGVCQGCHMTLPPQLNNILARMES
                     IEMCPRCGRLVYRKELLEGKPDAGAGSDGGSGSAAPQS"
     gene            complement(2988..3488)
                     /locus_tag="VMZ53_24255"
     CDS             complement(2988..3488)
                     /locus_tag="VMZ53_24255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31647.1"
                     /translation="MKRSLLLSVLVVASCNDDVDLTGIYKVDADVASMPCGTDQAVTN
                     PPVALKFGKGEFFGAEYFSYQECDDVAATMCSSGGLFDASFSEPIDGGWRGIVTSSGG
                     SGTMCTISYTEQTAVLHGSLLIIEMSQYSAQVDNTPTLCSTDEADRRNTSMPCGEHER
                     IEATKQ"
     gene            complement(3485..4786)
                     /locus_tag="VMZ53_24260"
     CDS             complement(3485..4786)
                     /locus_tag="VMZ53_24260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31648.1"
                     /translation="MNALWRALHKLGADRVDMYEIEQWGFSIHAALSAPGREFHNHDH
                     VIEIASTGDPLEIIAALYHDAVYIQVDQGPPRSMRAEMDPYLLPTEGGWLVQPAAAGD
                     AATADVLTVFGRRSGETITPTMGLNEFASALVCCRQLQHALARNHRIAVAACIEQTIP
                     FRTDPVNTLRDRLMSLGISGDELDDILKRAVRIGNRDVENFAENDAARFLDNTWKLLP
                     ETNPALHSPMVYTVRDYRTALQKMESFLAVLPAERVFHTWKDEPKPEIHARRVARTTG
                     NLELAVRYLRAKLYSIALVEALAELTGGDVPLDYFMGSPKTSANPDVKRIENYLPHMG
                     SAKDLDPPLHRLLAEGRASESSFDTKQAPIGAFLHSTVGEAAVMKGVDSAKRWWAGAG
                     DAQAFIEAQPRKPVAAIARAAANIIDTRRDRLFALAERLEA"
     gene            complement(4851..5204)
                     /gene="rnpB"
                     /locus_tag="VMZ53_24265"
     ncRNA           complement(4851..5204)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VMZ53_24265"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5283..8318)
                     /locus_tag="VMZ53_24270"
     CDS             complement(5283..8318)
                     /locus_tag="VMZ53_24270"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HUS31649.1"
                     /translation="MARPKSPASKKPVAKKSSAEPQSKNPPVAKRDAAPLDDDKAAAK
                     APRDDKAAAKAPRDNAKAPRDDKAAAKAPRDNARDKAAAKAASPALPPPKPPKPNPKP
                     AQGPDGPTIDSVAKEGAEDGGGDDEHGSVGGGLFSRAAARVGKLFGSGRKTGAGADAK
                     DGGPRGAKTGTNGNAERGGKASASIVDARNADGNGRTSKSDGGNGNGRTSKSDGRGGT
                     AKSDGRGRNAGANGNGRSKSNGASNGPDRLPPSGETHQTATDEILTTNQGVPVSDDQN
                     SLKLGARGPTLLEDFHFLEKMTHFDHERIPERIVHARGSAAHGTFTPYKSLSKLTSAA
                     FLQNAGKETPVFVRFSTVAGGAGSVDLPRDVRGFAVKFYTDEGVFDLVGNNIPVFFIQ
                     DALKFPDLVHSVKMEPDRGYPQNATAHDTFWDFVSLMPESMHMLMWAMSDRTIPRSYR
                     MMEGFGVHTFKLVNAQGDVTFVKWQWRPVIGSQSVVWDEAVKINGADQDFHRRDLFMA
                     IQAGAFPEWELSIQAFTEDDIADFPFDILDATKFIPEELIPLQPIGKMVLDRWPDNFF
                     AETEQVAFHPGHLVPGIDFTDDPLLQGRIFSYTDTQLKRLGSPNFHEIPINRPRCPMR
                     NFQRDGHMRQEINRGRVNYEPNSLATSFPRETPDGFVTSPASFAPPSGFGQAAKVRER
                     SETFADHYSQARQFWHSMTEPEQRHIVNAFTFELAKVDTLAIRTRMLGHLNIIEPELG
                     ARVADGLGMVGQAEAITPVREPIDLEPSPSLSILAKHVPTLQGRKVGCMIGDGFDRAL
                     VEKLRAAVVKEGAMFELVAPKIGGALAADGTQMPADHMILGAPSVLFDAVVVAPGAAA
                     LPTLMIESAAKQWVFDAFTHCKVIGVVGAAQPLLDAAGVMPDDGIVAITGKSLDAYVA
                     RVKQGRIWTREVITMGPDKKLSTSKTPVLTSGKPRYAPEDDMAKAAKPKKKTPPPPPP
                     AKAGKPKSGGKAAGGPQMGIGDFMQEQFEERAGKKPKRG"
     gene            8510..9736
                     /locus_tag="VMZ53_24275"
     CDS             8510..9736
                     /locus_tag="VMZ53_24275"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HUS31650.1"
                     /translation="MDARISALQKEAATRDDVIGLSGGLPADDLMPKDELGRALAYVA
                     TRRDEALQYGWPEGTKQLRAWIADRLAGRGATIDPERVIITAGAQQALSLAAQMVAAG
                     TSSQANIAASGSSTRDARIETGAATYPGALDAFRGANVQVIARCDADKQRTRHGSGDK
                     LPPRDAAAGSRDTRAAGGGGCVRYLTAGVDMPTGVAVDHRDLLDRDEPLIVDEAYAEL
                     RFDGSVPRPLIADAPERVWHVGTISKTIAPGLRVGWLIPPEAEHDAVLELKSAADLHT
                     ASISQAALSRLLGTLDYDALVARARAAYADRAERAVRALRQHASGLEFAEPAGGFSIW
                     IETGERRDEADFLEAALAERVMVDPGSLFRPEPDDRLAFRISYSNAPIDVIDEGIRRL
                     ARALRSSKVVARAASR"
     gene            complement(9737..10495)
                     /locus_tag="VMZ53_24280"
     CDS             complement(9737..10495)
                     /locus_tag="VMZ53_24280"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HUS31651.1"
                     /translation="MSDLAFVDLSIIVPAYNEEQRLAPTLEKLHAFLQTQPLRYEIVV
                     VDDGSKDKTCDLVISMMPRITGLRLVRQNPNRGKGAAVRRGMLDARGQIRVMCDADGS
                     MPAEELPKLLAPIVQCKASIAIGSRYAEGAKTDKKQPFYRVLWSRLCNRVIQKSLVPG
                     VRDTQCGFKAFTAEAARDLFRYGRIDGWAFDLEILALARRRGFEIAEVGVEWMDDGRS
                     RVNPLKDMWKVVSEAMTIRKNLKAGVYDQRALAA"
     gene            complement(10507..10902)
                     /locus_tag="VMZ53_24285"
     CDS             complement(10507..10902)
                     /locus_tag="VMZ53_24285"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HUS31652.1"
                     /translation="MLRAGFGGVAATATDVTLLVLLVKHLHVPVAVGSFLGAAAGAAV
                     GFALNKYIAFRDRSPITFKQVGRFGLVAVATALLMAFAMQLIAVKLGAPLLVAKLVCS
                     ALVFIAWTYPAQRRLVFRRPRPQPWMSLS"
     gene            11142..13232
                     /gene="malQ"
                     /locus_tag="VMZ53_24290"
     CDS             11142..13232
                     /gene="malQ"
                     /locus_tag="VMZ53_24290"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HUS31653.1"
                     /translation="MAVDLRDLARAAGFDPEYTSWRGERVASSEETLRMALATIAPHL
                     GVDIERDDAIRAIENERWKEMVPPVVLAWDGRLALPFHVPAEFDDDWECEVTLEDGGT
                     VRAHGRLFDLPAEGHAWPGGVVHCRRTASVWLEGQLGYHSVTWKTAGGEGEALVIAAP
                     SKAAGQPGYGMKRWGVFAPVYGLASRESGAAGDLRTLRMLMEQVGKRGGKYVATLPIL
                     AAFLDEPYAFSPYSPASRLFWNELYLDLHAIAAEIGVAVPAPPPIAVNEPIDYRAQYR
                     WRRPVIDQVAQRLIRDPAFVQGIDEWARETGAYDYAAFRAIGERERSGWHSWPVAVRD
                     GGAGIATSRERAWELAGGGEREKASVDTHLVAQWLMQSQLEQMHGGDAQLYLDLPVGV
                     NCDAYEVWRFRKLFLLDLAAGAPPDALFLGGQNWGLPPLSPHALRRDRYRYFIRCLRH
                     HMRTAGMLRIDHVMGLFRLYCVPSGRPATDGVYLRYPYEELIAIVTLESRRSHCAVTG
                     EDLGTVPENVRPTMTRHGLFRLHVGQWSFPSEPGKSPEASPAEAVASLNTHDTATFAG
                     WWRGLDIDDQRDLGLINAEQEAQQRAEREKQKLAILAFAGPNAAEDTLTEVERMMVAV
                     TTDLALGPAEIVLATIDDLVLDPVPHNVPGTVSERPNWQRRLQNWSQVLDPERAPPAA
                     AAAVTALIAARPSS"
     gene            complement(13242..16373)
                     /locus_tag="VMZ53_24295"
     CDS             complement(13242..16373)
                     /locus_tag="VMZ53_24295"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31654.1"
                     /translation="MGALLAERYEILRELGRGGMGEVLLARQVNIGRLVVIKRVLGEL
                     ASEKHVRMLLEEAQIAARLHHPCIVAVLDVSREVSEAFIAMEYVAGVSLRELLVKAPT
                     GLPKEVALQIAFDVLRGLDYAHGVKSGEFVGVVHRDIKPRNVMVTFAGDTKVIDFGIS
                     RWLAGDGAWESTSVSGTHGYMPPEQQQGKRVDARADQYAIGITLREMVSGISPREAEA
                     TVQAGKPAAPRGDVDEELEAIIDRATAHDPDNRFASCAALSGALQLYAKQRGVTPSPL
                     AVERWLRENLAERIATWERDAARASEPSMPQLLNATQRTGKPVAIDSTGKPAPADIVG
                     DTAGAVLTSGARTVPDTGKAIVSSGARTMPAGPSDESPTTARSSLGERLTPPPTRKHT
                     VRRRWPAIAAAGVLLVVGGGGAAWWATRTPAATAPPPPALVSVTAINEGSADDAWLAP
                     AIEHLGTQAMRDVPERRFPVARDNDPRPSRHIVLAYRHTPAGLQIEARVVDTASKKSS
                     AMLARGAGPSVADALEQLVPPLVKALGEGQPDPAPDDEERTQMAAVGASSLAQYRRFR
                     ALSVETRLAGWIDSDRYATYFDELVKADPTWAHPYGDLFWLRGSGTSPVASKLLADAR
                     AKVDGKRDPSGVKLLDSLEAERVANYALMEKLSAPLFEANNEDMLAGEALYAALSNMH
                     RGDDGRAVFRRLHERFPSLYFVADIGTALIEEGREDQAQRVVSDALASHPENLTASRE
                     VVRSHARKGELDDAKRVATRALLVHGERPVALAELFEMMVLAGDLSEAQRLVDAMLLG
                     SPLSKARGRYRQAVLAIFEGRLNAATLSARQAIEAYGGAAGEESEVGQALMLLRSVGD
                     PAAARAATTQFAELITSQDDPETATVYRYRAELVPGAKNVCPKIEPYLAKLDEVDRIE
                     ARRDITRSAAMAGCAPCKDVLAAGFSDGELSAESLILHGDCAMKERDLPIAKRAYEQA
                     TQLWSSWWSSEASPYYAILAHQRLARVAHELGDNATARREYERFLAAWGKPDRPIAEA
                     DAARRELAALPAP"
     gene            complement(16400..>17491)
                     /locus_tag="VMZ53_24300"
     CDS             complement(16400..>17491)
                     /locus_tag="VMZ53_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HUS31655.1"
                     /translation="EEFRVQKVLGFQGLKRFELTEAVAGNIVAFTGMENLNVGETITS
                     IEKPTILPLLKIDAPTITMNFRVNDGPFAGKEGKYVTSRNLAERLHRELKSNVALRVE
                     DTADAGVFRVSGRGELHLTVLIETMRREGYELCVSQPKVILQEDANGKITEPYEDAVI
                     DLDENYVGAVVAELNRRLGIMREMRPSAPGRTRLEYRIPARGLIGYRSQFMTDTRGTG
                     VLYTQFAEYGPQGADIRTRQNGVLISSEDGISNAYGLFYLQERGQMFIGPSVKNYSGM
                     IIGIHARDNDLVVNPNKAKKLTNIRTTAADEKLVLAPPRQLTLEYALEFINDDELCEV
                     TPQSIRLRKMILDHNVRKRFEKKPAEIDD"
BASE COUNT         2760 a         5715 c         5982 g         3034 t
ORIGIN      
        1 ttcgccatct accggcggtg ccaccggttc gcccgctccc cgatcggcgc aggtgatctc
       61 ttgcgacaac cgcgctaggg tctgggcgta tgcgctttgc gaagtatcag ggcctcggca
      121 atgacttcct cgtcgtcgat ctgcgcgacg tgcgcggcgc ggaggccgat gcggcacaga
      181 atcccgacgt cgtgaaagcg ctgtgcgatc gccagttcgg cgtcggcggt gatggcgtgc
      241 tggcggtgtt gcccggcgac gccggggcgg atgcgcgcat gcgcgtgctc aactccgacg
      301 gctcggaagc cgagatgtgc ggcaacggca ttcggtgcgt cgcgaaagag ctttatgacc
      361 gcggcggtgt gaagaaagat cgcatcgcga tcgataccgg cgcgggtcgc ctggtgtgcg
      421 cgatctccga gaagggtggc gtcgctgaga cggtcaccgt cgagatgggc gcgccgcggc
      481 tgctgcgcgg cgagattccg atgaccgggc ccgcgaacga gcgctgcatc gagcagccgc
      541 tgtcgctgtc cgccgactcg accgagctgc agattacggc cgtgtcgatg ggcaacccgc
      601 acgcggttgc gtttgtggcg ggcaagcaga tgcagctcgc gacgacgatc ggaccgcgcg
      661 tcgagaatca cgcgtggttt cccaagcgca cgaatgcgga gttcgcgcgc gtgatttcgc
      721 cgaccgagat cgagctcgtc gtgtgggagc gcggctgtgg aattacgctc gcgtgtggca
      781 ccggtgcgtg tgcgaccgtc gttgccgcag tcgtgacggg ccgcgcgcaa gaaggcagcg
      841 atgtgctcgt gcacttgccg ggcggcgatc tgacgatcac cgtgctgccg aatttcgcga
      901 acgtcctgat gaaggggccc gcgcgtcacg tgttcgacgg cgagctcgac ccgcgccgcg
      961 tgacgcctcg ccccgcgtga ccggaaatta ggtccgaccg aatttccacg cagtcgcgcc
     1021 gaaacgacaa acgccgccga ggtttctcga cgcgttggtg cgttccgaaa tgcgaaacgc
     1081 cgccgaggtt ttctcgacgg cgttggtgcg tctgcgcgag gcgcgcgacg ttcagcgcgg
     1141 cgggtagtac ggaccctgcg gctgcggcgc gggctgctgc gggtagtacg gctgcgtcgg
     1201 ctgctgcggg ccgtacgggc tcggctgctg cggaccgtac gggtttggct gctgcggctg
     1261 cggctgctgc gggtagtacg gctgcgtcgg ttgctgcggt tgttgcgggt actgctgcgg
     1321 atattgctgc ggatactgcg gctgctgtgg ctgttgtggc tgcggcggac gaccgtacgt
     1381 ttggccgccg tattggctgt tcatgtacgg gttctgcccg ccgtagggct ggccaccata
     1441 ttgcggcgcg ggttgcgacg agacggcttg gcgccagcca ttccagtcgc ccacgcgatc
     1501 gcgcttgtca ctccaccagc ggcccgagta ttggcggccg gatggatcga agacgagata
     1561 gccttcgccc gtgagcggcg ggttgttcgg ctcttgccag cggaagttga agacgttgcc
     1621 gcgcagcgag ccgttgaagt agccgacgac ttcttggcct tggcgctgat agacccagac
     1681 gccttggatc gcgcccgact gaactccgcc gcgcgagttg tcggcttcga tcttcaccgc
     1741 gccgaatgtc gatcgccaga gaccgagcgc tcgcgcgggc tccatctcgc gaggaggcgg
     1801 cgggctcatg ttcgcgttgg cgccctgcgc ctgcgtagcc ggcgggttgc tgctgttcga
     1861 ggttgccgag ccgactgctg cgaggcatgc agcggaaacc gcgattaccc gtgcgacgtt
     1921 gccgaatctc ataggatttc gagtatagcg ccgcgcttgg cgtaaaaccg ctgacctgct
     1981 agtgtccgct cgcgtttccg tgcggctttc cacacgaggt ggcagcggcg gcacgcgggc
     2041 ccatagctca gtcggttaga gcggccggct cataaccggt gggtccctgg ttcgagccca
     2101 ggtgggccca cgacccgagt gaaaatcagc agaagtccca gcagcgaaag cgaaaggttg
     2161 tctacgtgcg cgatcagctt gtgtacctac tcgagctcca gaattttgac ggcaaggttc
     2221 gcgagctcga tgcggccgcc aagcagcttc cggcaaagct cgacccgctg cggcgcgacc
     2281 tggcaaagct gcaaggcatg ctcgatgccg agcgggcgaa gatggccgag acgcaaaact
     2341 ggcggaagtc gcagcaggag ctgatcgacc gcgagcgcga agcgctcaag agcgcgcaga
     2401 gcaagttcca ggcgtcgaag aacacgaagg agttttcggc cgccagccgc gaggtcgaga
     2461 acaagcgcaa gtcgattcaa gatcgcgagg gtgagctgaa gaaggtcacc gaggcgacga
     2521 acgcgcagga gaccgcgctg tcggcgcgcg atgccgacgt gcagaagctt cgcgacgagc
     2581 tcgcagccgg tgaagcggcg atggccgatc aggtcaagag cgtcggcgat cagctcgccg
     2641 aagcgaaagc cgcccgcgat gcagcgcgca gcaaggtcga gaagaagtgg ctgtcgacgt
     2701 acgactcact cgcggccaag cgcggctacg ccgtggcgcc cgtgatcaaa ggcgtgtgcc
     2761 agggctgcca catgacgctg ccgccgcagc tcaacaatat cttggcgcgc atggaatcga
     2821 tcgagatgtg cccacgctgc ggccgcctcg tgtatcgcaa agagctgctc gagggtaagc
     2881 ccgacgcggg cgccggcagc gacggcggca gcggcagcgc tgctccgcag agctgagcgt
     2941 tcgtcgacgc gccgccaccg cagcgactct gcgtcgctcg agcagcgcta ctgcttcgtc
     3001 gcttcgatgc gctcgtgctc gccgcacggc atcgacgtgt tgcgacggtc ggcttcgtcc
     3061 gtcgagcaga gcgtcggcgt gttgtcgacc tgcgcggagt actggctcat ctcgatgatg
     3121 agcagcgagc cgtgcagcac ggcggtctgc tccgtgtagc tgatcgtgca catcgtgccg
     3181 ctgccacccg acgacgtcac gatgccgcgc cagccgccgt cgatcggctc ggagaacgac
     3241 gcgtcgaaca ggccgcccga cgagcacatc gtcgccgcga cgtcgtcgca ttcttggtaa
     3301 ctaaagtatt cggcgccgaa gaattcgccc ttgccgaact tcagcgcgac cggcggattc
     3361 gtgacggctt ggtccgtgcc gcacggcatc gacgcaacat ctgcatcgac cttgtagatg
     3421 cccgtcagat cgacgtcgtc gttgcatgag gcgacgacga gcacgctcaa aagcagcgac
     3481 cgcttcacgc ttcgaggcgc tccgcgagag cgaacaggcg atcgcggcgc gtatcgatga
     3541 tgttcgcggc ggcacgcgcg attgcggcga cgggcttgcg cggctgcgct tcgatgaacg
     3601 cttgcgcgtc gcctgcgccg gcccaccagc gcttcgcgct gtcgacgccc ttcatgacag
     3661 cagcctcgcc gaccgtcgag tggaggaatg cgccgatcgg cgcctgcttc gtatcgaagc
     3721 tcgattccga cgcgcggccc tctgcgagga ggcgatgcag cggcggatcg agatccttcg
     3781 ccgagcccat gtgcggcagg taattctcga tgcgtttgac gtcgggattg gccgatgtct
     3841 tcggacttcc catgaagtag tcgagcggca cgtcgccgcc ggtcagctcg gcgagcgcct
     3901 cgacgagcgc gatcgagtac agcttcgcgc gcaggtagcg caccgcaagc tcgaggttgc
     3961 ccgtcgtgcg agcgacgcgg cgcgcgtgaa tctcgggctt cggctcgtct ttccacgtgt
     4021 ggaacacgcg ctcggccgga agcaccgcga ggaatgactc catcttctgg agagccgtgc
     4081 ggtagtcgcg caccgtgtag accatcggcg agtgcagcgc cgggttcgtc tcgggcagca
     4141 gcttccacgt gttgtcgagg aatcgagccg cgtcgttctc ggcgaagttc tcgacgtctc
     4201 gattgccgat gcgcaccgcg cgcttgagaa tgtcgtcgag ctcgtcgccc gagatgccaa
     4261 ggctcatcag ccgatcgcgc agcgtgttga ctggatcggt gcggaacgga atcgtctgct
     4321 cgatacacgc ggcgaccgcg atccggtgat tgcgcgcgag cgcgtgttgc agctggcggc
     4381 agcagaccag cgccgacgcg aactcgttga gccccatcgt cggcgtgatc gtctcgcccg
     4441 agcggcggcc gaacaccgtg agcacgtccg cggtcgctgc gtcgccggcc gcggcaggct
     4501 gcacgagcca gccaccttcc gtcgggagaa ggtacggatc catctccgca cgcatcgagc
     4561 gcggcgggcc ctgatcgacc tggatgtaga ccgcgtcgtg atagagcgcg gcgatgatct
     4621 cgagtggatc gcccgtcgag gcgatctcga tgacgtgatc gtggttgtga aactcgcgtc
     4681 cgggcgccga cagcgcagcg tggatcgaga agccccactg ctcgatctcg tacatgtcga
     4741 cgcgatcggc gccgagcttg tggagagccc gccacagcgc gttcacgatt tcgtgtaccg
     4801 gtgaccgcgc gtccatagcc ctcacagcca cggtatcaga aaaggctcac gcgtcggaga
     4861 gggcggtaag ccgagttctg tatgtggcga ccattcttct aggcccagcg ttgccactgg
     4921 gctcgagcga ccaacccgga gtcatcgcgc gggccacgcg acccctgtct ggtcttgctt
     4981 cgggtggggt ttacctagcc tccactgtca ccagcagagc ggtgcgctct taccgcaccc
     5041 tttcaccctt acctgccttg cgacaggcgg tttgctttct gttgcactgt cctgcgagtc
     5101 acctcgactg gtcgttaacc agcaccctgc cctatgaagc tcggactttc ctctcgcgcc
     5161 gatgcgttgc cgcaccgtca ccagcggtca cctcgccctc tccgacgaat gagcgacgca
     5221 aagctactca ctcgcgctca cgcgagcaag cgcgttcgga gattgatggg cagcgccgcg
     5281 agttagccgc gcttgggctt cttgcccgcg cgctcttcga actgctcttg catgaaatcg
     5341 ccgatgccca tctgcgggcc gccagcggcc ttgccgccgc tcttcggctt tccggccttc
     5401 gcaggaggag gaggaggagg cgtcttcttc ttcggcttcg ctgctttcgc catatcgtct
     5461 tctggagcgt agcgcggctt gcccgacgtg agcacgggag tcttgctcgt cgacagcttc
     5521 ttgtcggggc ccatcgtgat gacctcgcgg gtccagatgc ggccctgctt cacgcgcgcg
     5581 acgtaggcgt cgagcgactt gccggtgatc gcgacgatgc cgtcgtcggg catcactcct
     5641 gcggcatcga gcagcggctg cgccgcaccg acaacgccga tcaccttgca gtgcgtgaac
     5701 gcgtcgaaca cccactgctt ggccgccgat tcgatcatta gcgtcggcaa cgccgctgcg
     5761 ccgggcgcga cgaccacggc gtcgaacaac accgatggcg caccgaggat catgtgatcc
     5821 gccggcatct gcgtgccgtc cgcggcgagc gcgccgccga tcttcggagc gacgagctcg
     5881 aacatcgcgc cctctttgac gaccgctgca cgcagcttct cgacgagcgc gcgatcgaag
     5941 ccatcaccga tcatgcagcc gaccttgcgg ccctgcagcg tcggtacgtg cttcgcgagg
     6001 atgctcagcg acggcgacgg ctcgagatcg atcggctcgc gcaccggcgt gatcgcttcg
     6061 gcttgaccga ccatgccgag gccatcggcc acgcgtgcac cgagctcggg ctcgatgatg
     6121 ttgaggtgac cgagcatgcg cgtgcgaatc gcgagcgtgt cgaccttcgc gagctcgaac
     6181 gtgaacgcat tcacgatgtg gcgctgctcg ggctccgtca tcgagtgcca gaactggcgc
     6241 gcttggctgt agtgatcggc gaacgtctcg ctgcgctcgc gaaccttcgc ggcctgacca
     6301 aagccgctcg gcggtgcgaa cgacgcaggg ctcgtgacga acccatccgg cgtctcgcgc
     6361 gggaagctgg tcgcgagcga gtttggctcg tagttcacgc ggccgcggtt gatctcctgg
     6421 cgcatgtgac cgtcgcgctg gaagttgcgc atcgggcagc gcggccgatt gatcggaatc
     6481 tcgtggaagt tcggcgagcc gaggcgcttc agctgcgtgt cggtatacga aaagatgcgg
     6541 ccctgcagca gcggatcgtc ggtgaagtcg atgccgggca cgaggtggcc ggggtgaaac
     6601 gcgacctgct cggtctccgc aaagaagtta tccggccagc gatcgagcac catcttgccg
     6661 atcggctgca gcgggatgag ctcttcgggg atgaacttcg tcgcgtcgag gatgtcgaac
     6721 gggaagtcgg cgatgtcgtc ctcggtgaat gcctggatcg agagctccca ctcggggaac
     6781 gcgccggcct ggatcgccat gaacagatcg cggcgatgga agtcttgatc ggcgccgttg
     6841 atcttcaccg cctcgtccca cacgaccgac tgcgagccga tcaccggacg ccattgccac
     6901 ttcacgaacg tgacgtcacc ctgcgcgttg acgagcttga acgtgtgaac gccaaagccc
     6961 tccatcatgc gatacgagcg cgggatcgtg cgatccgaca tcgcccacat cagcatgtgc
     7021 atcgactcgg gcatgagtga cacgaagtcc cagaaggtgt cgtgcgcggt cgcgttctgc
     7081 ggatagccgc ggtccggctc catcttcacg gagtggacga ggtcgggaaa cttcagcgcg
     7141 tcttgaatga agaacaccgg gatgttgttg ccgacgagat cgaagacgcc ctcgtccgta
     7201 tagaacttca cggcgaagcc acgcacgtcg cgcggcagat cgacagagcc cgcaccgccg
     7261 gcgacggtcg agaaccggac gaacaccggc gtctccttgc cggcattctg cagaaacgcc
     7321 gcgctcgtca gcttcgagag cgacttgtac ggcgtgaacg tgccgtgcgc cgccgacccg
     7381 cgcgcgtgaa cgatgcgctc gggaatccgc tcgtgatcga agtgcgtcat cttctcgagg
     7441 aagtggaagt cctcgaggag cgtcgggccg cgggcgccga gcttcagcga attctgatcg
     7501 tcggagaccg ggacgccctg gttcgtcgtc aggatctcgt cggtggcggt ctgatgcgtc
     7561 tcgccgctcg gcggcagtct gtcggggccg ttcgatgcgc cgttcgactt gctgcggccg
     7621 ttgccattcg cgccggcatt gcgaccgcgg ccatcgctct tcgctgtgcc gccgcgacca
     7681 tcgctcttcg atgtgcggcc gttgccattg ccgccatcgc tcttcgatgt gcggccattg
     7741 ccgtcggcgt tgcgcgcgtc gacgatcgag gcgctggcct taccgccgcg ctccgcgttg
     7801 ccattcgtgc cggtcttcgc gccgcgcgga ccaccgtcct tggcgtctgc gccggctccg
     7861 gtcttgcgac cgctgccgaa cagcttgccg acgcgagccg ccgcgcgcga gaacaagccg
     7921 ccgccgacgg agccatgctc gtcatcgccg ccgccgtctt cggcgccttc tttcgccacg
     7981 ctgtcgatcg tcggaccatc ggggccttgc gccggcttcg ggttcggctt gggcggcttc
     8041 ggcggcggaa gcgcgggtga agcggctttc gctgctgcct tgtcgcgagc attgtcgcgc
     8101 ggtgccttcg cggccgcctt gtcgtcgcgc ggtgccttcg cattgtcgcg cggtgccttc
     8161 gcggcggcct tgtcgtcgcg cggggccttc gcggcggcct tgtcgtcgtc gagcggtgcc
     8221 gcatcgcgct tcgcgaccgg cgggttcttc gattgcggtt cagcggaaga cttcttcgca
     8281 acgggcttct tcgatgcagg cgacttcggg cgagccatgg ttcgcgccct tcgagcaagt
     8341 cgtgtaccgc tgtgcattcc gcgcacgctc cgcattcctc gacgacgagc cgcgatcgga
     8401 tctgctcaga ccgagcgctt acgctccgtc cgtgcgaagt cgcgcagcgg ctgcgacaag
     8461 cttgcgcgca atgtcgcggc gatgaacgag ctgcgtgcta gccacaagca tggacgcgcg
     8521 catttcggct ctacagaagg aagccgcgac gcgcgacgac gtcatcggcc tgtcgggcgg
     8581 actcccggcc gacgatctga tgccgaagga cgagctcggc cgcgccctcg cctacgtggc
     8641 cacgcgtcgt gatgaagcgc tccagtacgg ctggcccgaa ggcacgaaac agctccgcgc
     8701 atggatcgcg gaccgcctcg ccggtcgcgg agcgacgatc gatccggagc gcgtgatcat
     8761 cacggccggc gcgcaacaag cgctgtcgct cgcggcgcag atggtggcgg ccggcacgag
     8821 ctcacaagcc aacatcgcgg cgtcgggctc gagcacccgg gacgcgcgca tcgagaccgg
     8881 tgcggcgacc taccccggcg cgctcgacgc atttcgcggc gcgaacgtgc aggtcatcgc
     8941 gcgctgtgac gccgacaagc agcgcacgag acatggcagc ggcgacaagc tgccgccccg
     9001 cgatgccgca gcaggcagcc gcgatacgcg ggccgcgggg ggcggcggtt gcgtccgtta
     9061 cctcaccgcg ggcgtcgaca tgcccaccgg cgttgcagtc gaccatcgcg atcttctcga
     9121 tcgcgacgag ccgctcatcg tcgacgaggc gtatgccgag ctgcggttcg acggcagcgt
     9181 gccgcggccg ctgatcgcag atgcgcccga gcgcgtctgg catgtcggta cgatcagcaa
     9241 gacgatcgcg ccgggcctgc gcgtgggctg gctcatcccg cccgaggcgg agcacgatgc
     9301 ggtgctcgag ctgaagtcag cggccgatct gcacaccgcg agcatcagcc aagcggcgct
     9361 gtctcggctg ctcggcaccc ttgactacga cgcgctcgtc gcgcgggctc gggcggcgta
     9421 tgccgaccgc gccgagcggg cggtgcgggc gttgcgccag catgcaagcg ggctcgagtt
     9481 tgcggagcca gccggcggtt tctcgatctg gatcgagaca ggcgagcgcc gcgacgaggc
     9541 cgactttctc gaagccgccc tcgccgagcg cgtgatggtc gatccgggca gcttgtttcg
     9601 ccccgagccc gatgaccggc tcgcgttccg cattagttac tcgaatgccc ccatcgacgt
     9661 catcgatgag ggcatccggc gtcttgcgcg cgcgctgcgc agcagcaagg tcgtcgctcg
     9721 cgctgcgagc cggtagctac gccgcgagtg cgcgctgatc gtagacgcct gccttgaggt
     9781 tcttgcggat cgtcatcgct tcgctgacga ccttccacat gtccttgagc ggattgacgc
     9841 gcgagcggcc atcgtccatc cactcgacgc cgacttccgc gatctcgaag ccgcgacggc
     9901 gcgcgagcgc gaggatctcg aggtcgaacg cccagccgtc gatgcggccg tagcggaaca
     9961 agtcgcgcgc cgcttctgcg gtgaacgcct tgaagccgca ctgcgtgtcg cgcacgccag
    10021 gaacgagcga cttctggatc acgcggttgc agagacggct ccacagcacg cgatagaacg
    10081 gctgcttctt gtcggtcttc gcaccttcgg catagcgcga gccgatcgcg atcgacgcct
    10141 tgcactgcac gatcggcgcg aggagctttg gcagctcttc cgccggcatc gagccgtcgg
    10201 cgtcgcacat gacgcggatc tggccgcgcg catcgagcat gccgcgacgg accgccgcgc
    10261 ccttgccgcg gttcgggttc tgccgcacga ggcgcagccc cgtgatgcgc ggcatcatcg
    10321 agatgacgag gtcacacgtc ttgtccttcg agccgtcatc gacaacgacg atctcgtaac
    10381 ggagcggctg cgtctggaga aatgcgtgca gcttctcgag cgtcggcgcg aggcgctgct
    10441 cttcgttgta cgcgggaaca atgatcgaga ggtcgacgaa tgcgaggtca gacatgacag
    10501 gctccttcac gaaagcgaca tccacggctg tggacgaggg cggcgaaaga cgagacgacg
    10561 ctgggcggga tacgtccaag cgataaagac gagcgcagag cacacgagct tcgcgacgag
    10621 cagcggcgcg ccgagcttga cggcgatgag ctgcatcgcg aacgccatca agagtgcagt
    10681 cgccacggca acgagaccga agcgaccgac ctgcttgaag gtgatcggcg agcgatcacg
    10741 gaacgcgatg tacttgttca acgcaaagcc caccgcggca ccagcagctg cgccgaggaa
    10801 gctgccgaca gcgaccggga catggagatg cttgaccagc aggacaagca gcgtcacgtc
    10861 cgtcgcggtg gcggctacac cgccaaaccc ggcacggagc atctcgcgaa gttgcatgac
    10921 ccaggcaatg agcaccggat gtgccatggg cgctcggtgc ggcaaacccg cgaaaccgcg
    10981 cgtcaaagct ggctacctgc gcggtgaaag gtggtgaaaa gctatcgagc tgacgtcagg
    11041 tgaacttcgc gtgatcgcgg gaatgaacgg tcttggtcgg cctgcccgat tcacgctgca
    11101 cgcagcgggc gctcccgatc tcgctttggt acggtgcccg cgtggctgta gaccttcgcg
    11161 atctcgcacg cgcggccggc ttcgatcccg agtacacgag ctggcgcggc gagcgcgtcg
    11221 cttcgagcga agagacgctg cgcatggcac tcgcgacgat cgcgccgcat cttggcgtcg
    11281 atatcgagcg cgacgatgcg atcagggcga tcgagaacga gcgctggaaa gagatggtcc
    11341 cgccggtcgt gctcgcgtgg gacggtcgat tggcgctgcc gtttcatgtg cccgcagaat
    11401 tcgacgacga ttgggagtgc gaggtcacgc tcgaagatgg cggcacggtg cgcgcgcatg
    11461 ggcggctctt tgatctgccg gccgagggac acgcgtggcc gggcggcgtc gtgcattgcc
    11521 ggcggaccgc gagcgtgtgg ctcgaagggc agctcggcta tcactcggtg acgtggaaga
    11581 cggccggcgg cgagggagag gcgctcgtga ttgcggcgcc gtcgaaggca gccgggcagc
    11641 cgggctatgg catgaagcgg tggggcgtgt tcgcgcccgt gtacgggctt gcatcgcgcg
    11701 agagcggcgc ggcgggagat ctgcggacgt tgcgcatgct catggagcaa gtcggcaagc
    11761 gcggcggcaa gtacgtcgcg acgctgccga tcttggcggc gtttctcgac gagccgtacg
    11821 cattttcgcc gtactcgcct gcgagccgtc tgttctggaa cgagctgtat ctggatctgc
    11881 acgcgatcgc ggcagagatc ggcgttgccg tgcctgcgcc gccgccgatt gccgtgaacg
    11941 agccgatcga ttatcgcgcg cagtatcgct ggcggcgccc cgtgatcgat caggtcgcgc
    12001 agcggctcat tcgcgatccg gcattcgtgc aaggcatcga cgagtgggcg cgcgagaccg
    12061 gtgcgtatga ctacgcggcg tttcgtgcga tcggcgagcg cgagcgatcg ggatggcaca
    12121 gctggccggt cgcggtgcgc gatggcggcg cgggcatcgc gacgtcgcgc gaacgtgcgt
    12181 gggagctggc cggcggcggc gagcgcgaga aagcgagcgt cgacacgcat ctcgtcgcgc
    12241 agtggctcat gcagtcgcag ctcgagcaga tgcacggcgg cgacgcgcag ctctatctcg
    12301 atcttcccgt cggcgtgaat tgcgacgcgt acgaagtgtg gcgcttccgc aagctgttct
    12361 tgctcgatct cgctgcgggc gcgccgcccg acgcactgtt cctcggcggg caaaactggg
    12421 gcttgccgcc gctgtcgccg cacgcgctgc gtcgcgatcg ctatcgctac ttcattcgct
    12481 gcttgcggca tcacatgcgc acagccggca tgctgcgcat cgatcacgtg atggggctct
    12541 tccgtctgta ctgcgtgccg tcgggacggc cggcgacgga cggcgtctac ctgcgctatc
    12601 cgtacgagga gctcatcgcg atcgtgacgc tcgaatcgcg gcgctcgcat tgcgcggtga
    12661 ccggtgagga cctcggcacc gtgcccgaga acgttcgccc gacgatgacg cgccacggcc
    12721 tgttccggct gcacgtcggc cagtggtcgt tcccgagcga gccaggcaag tcgcccgagg
    12781 cgtcgccggc agaagcggtc gcgagcctca acacgcacga tacggcgaca ttcgcaggat
    12841 ggtggcgcgg cctcgatatc gacgaccagc gcgacctcgg cctcatcaac gccgagcaag
    12901 aagcgcagca gcgcgccgag cgcgagaagc agaagctcgc gatcctcgcc ttcgccggac
    12961 cgaacgcggc cgaggacacg ctcaccgagg tcgagcgcat gatggttgcc gtcacgacgg
    13021 acctcgcgct cggtcccgcc gagatcgtgc tcgcgacgat cgatgacctc gtgctcgacc
    13081 cggttccgca caacgtgccg ggaacggtca gcgaacggcc gaattggcag cgccggcttc
    13141 agaactggtc gcaggtactc gaccccgagc gcgcgccacc ggcggcggct gctgcagtta
    13201 cagcgctgat cgccgcgcgg ccgtcgtcgt agcagttggc gtcacggagc cggtaacgca
    13261 gcgagctctc ggcgcgcagc gtcggcctct gcgatcggcc tgtcgggctt gccccacgcg
    13321 gcgaggaagc gctcgtactc gcggcgcgcc gtcgcgttgt cgccgagctc gtgcgcgacg
    13381 cgggcaagac gctggtgcgc gaggatcgcg taatacggcg aggcctcgct gctccaccag
    13441 ctcgaccaca gctgcgtcgc ttgttcgtac gcgcgcttcg cgatcggcaa gtcgcgttct
    13501 ttcatcgcgc agtcgccgtg caagatcagc gactcggcgg acagctcgcc gtcggagaag
    13561 cctgcggcga ggacgtcttt gcacggcgcg caaccagcca tcgctgcact gcgcgtgatg
    13621 tcgcggcgcg cctcgatgcg atcgacctcg tcgagcttcg cgagatacgg ctcgatcttg
    13681 gggcacacat tcttcgcccc gggcacgagc tccgcgcggt agcggtagac cgtggccgtt
    13741 tcgggatcgt cttgcgacgt gatgagctcg gcgaactgcg tggtcgcggc gcgcgcggcg
    13801 gcaggatcgc caacggaccg cagcagcatc agcgcttggc cgacttcgct ctcttcgcca
    13861 gcggcgccgc cgtatgcttc gatcgcttgc cgcgccgaga gcgtcgccgc gttgaggcgg
    13921 ccctcgaaga tcgcgagcac tgcttgccga taccggccgc gcgccttcga caacggcgat
    13981 ccgagcaaca ttgcgtcgac gagccgctgg gcctcggaga ggtcacctgc gagcaccatc
    14041 atctcgaaca gctcggccag cgcgaccggc cgctcgccat gaacgagcag cgcacgcgtc
    14101 gcaacgcgct tcgcgtcgtc gagctcgccc ttgcgagcgt gcgagcgaac gacctcgcgc
    14161 gaagccgtca gattctccgg gtgtgaggcc agcgcgtcgc tgaccacacg ttgcgcttga
    14221 tcctcgcggc cctcctcgat cagcgctgtg ccgatgtcgg cgacgaagta gaggctcgga
    14281 aagcgctcgt gcagccgccg gaacaccgcg cgaccgtcat cgccgcgatg catgttcgac
    14341 agcgctgcat agagagcttc gcccgcgagc atgtcctcgt tgttcgcctc gaacagcggc
    14401 gcggagagct tctccatgag cgcgtagttc gcgacgcgct cggcttcgag cgaatcgagg
    14461 agcttcacgc cgctcggatc gcgcttgccg tcgacctttg cgcgcgcgtc tgcgagaagc
    14521 ttcgacgcga cggggcttgt tccactgcca cggagccaga acaaatcacc gtagggatgc
    14581 gcccacgtcg ggtctgcctt gacgagctcg tcgaagtacg tcgcgtagcg gtcggaatcg
    14641 atccagcccg cgagccgcgt ctcgaccgat agcgcgcgaa accgccggta ctgcgcgagc
    14701 gatgacgcac cgacggcggc catctgcgtg cgctcctcgt cgtcgggcgc agggtcgggc
    14761 tggccttcgc cgagcgcttt gacgagcggc ggcacgagct gctcgagcgc atcggcgacc
    14821 gacggtccgg caccgcgagc gagcatcgcg ctgcttttct tcgaagcggt atcgacaacg
    14881 cgcgcttcga tctgcagtcc cgccggcgtg tggcggtacg cgagcacgat atggcgcgac
    14941 gggcgtggat cgttgtcgcg cgcgacgggg aagcggcgct cgggcacatc gcgcatcgct
    15001 tgcgtaccga gatgctcgat cgcaggcgcg agccacgcgt cgtcggcgct gccttcattg
    15061 atcgcggtca ccgagacgag cgcgggtgga ggcggagcgg tggccgcagg cgtgcgcgta
    15121 gcccaccacg ccgcgccgcc gccgccgacg acgaggagca cgccggccgc agcgatcgcg
    15181 ggccagcgcc gccgcaccgt gtgcttgcgc gtaggtggag gcgtcagccg ttcgccgagc
    15241 gagctgcgtg cggtcgtcgg tgattcgtcg ctgggcccag ccggcatcgt gcgcgcaccg
    15301 gagctcacaa tcgcctttcc ggtgtcgggc accgtgcgcg cgccggacgt caggaccgcg
    15361 cctgccgtat caccgacgat gtctgccggc gcgggctttc ccgtcgagtc gatcgcgacc
    15421 ggctttcccg tccgctgtgt cgcgttgagc agctgcggca tcgacggctc gctcgcccgc
    15481 gcagcatcgc gctcccacgt cgcgattcgc tcggcgaggt tctcgcgcag ccagcgctcg
    15541 acggcgagcg gcgacggcgt gacgccacgc tgcttcgcgt acagctgcag cgcaccggac
    15601 agcgcggcac agctcgcgaa ccgattgtcc ggatcgtggg cggtcgcgcg atcgatgatc
    15661 gcttcgagct cctcgtcgac gtcaccgcgc ggcgcggctg gcttgccggc ctgcaccgtc
    15721 gcctccgctt cgcgcggcga gatgccgctg accatctcgc ggagtgtgat gccgatggcg
    15781 tattgatccg cgcgcgcatc gacgcgcttg ccttgctgct gctccggcgg catgtagccg
    15841 tgcgtgcccg acaccgatgt cgactcccac gcgccgtctc ccgcgagcca ccgagagatg
    15901 ccgaagtcga tcaccttcgt gtcgcccgca aacgtcacca tgacattgcg cggcttgatg
    15961 tcgcggtgca cgacaccgac gaactcgccg ctcttcacac cgtgcgcgta gtcgaggccg
    16021 cgcagcacgt cgaacgcgat ctggagcgcg acctctttcg gcaagccggt cggcgctttc
    16081 acgagcagct cgcgcagcga cacgccggcg acgtactcca tcgcgataaa cgcctcgctg
    16141 acctcgcgac tgacgtcgag caccgcgacg atgcacggat ggtgcaaccg cgccgcgatc
    16201 tgcgcctcct cgaggagcat gcgcacgtgc ttctcgctcg cgagctcgcc gagcactcgc
    16261 ttgatgacga cgagccggcc gatgttgacc tggcgcgcga gcaagacctc gcccatcccg
    16321 ccgcgaccga gctcgcgcag gatctcgtat cgctcggcga ggagcgctcc cacgctccca
    16381 gtgtaccgct gtccgaaggc tagtcgtcga tttcggcggg cttcttctcg aagcgcttgc
    16441 gcacgttgtg gtcgagaatc atcttgcgca gccggatcga ctggggcgtc acttcgcaga
    16501 gctcgtcgtc gttgatgaac tcgagcgcgt attcgagcgt gagctggcgc ggcggcgcga
    16561 ggacgagctt ctcgtcggcg gccgtcgtgc ggatgttcgt cagcttcttc gccttgttcg
    16621 gattgacgac aagatcgtta tcgcgggcgt gaatgccgat gatcatgccg ctgtagttct
    16681 tgacgctcgg gccgatgaac atctggccgc gctcttggag atagaacagg ccgtaggcgt
    16741 tcgagatgcc gtcttcgctc gagatcagca cgccgttctg ccgcgtgcgg atatcggcgc
    16801 cttgcgggcc gtactcggcg aactgggtgt agagcacgcc ggtgccgcgc gtgtccgtca
    16861 tgaactgcga gcggtagccg atgaggccgc gtgccgggat ccggtactcg aggcgcgtgc
    16921 gacccggtgc cgaaggacgc atctcgcgca tgatgccgag gcgacggttc agctcggcga
    16981 cgaccgcgcc gacgtagttc tcgtcgagat cgatgacggc gtcttcgtac ggctcggtga
    17041 tcttgccgtt ggcgtcttcc tggagaatca ccttgggctg cgacacgcac agctcgtagc
    17101 cttcgcgccg catcgtctcg atgaggacgg tcaggtgcag ctcgccgcga cccgagacgc
    17161 ggaacacgcc ggcgtcggca gtgtcctcga ccctgagcgc gacgttgctc ttgagctcgc
    17221 ggtggaggcg ctcggcgagg ttgcgcgacg tgacgtactt gccttctttg ccggcgaacg
    17281 gtccgtcgtt cacgcggaag ttcatcgtga tcgtcggcgc gtcgatcttg aggagcggca
    17341 ggatcgtcgg cttttcgatc gaggtgatcg tctcgccgac gttgaggttc tccatgcccg
    17401 tgaacgcgac gatgttgccg gccacggcct cggtgagctc gaagcgcttg aggccctgga
    17461 agccgaggac cttctggacg cggaactctt c
//
LOCUS       DATLBZ010000479         5564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2102705, whole genome shotgun sequence.
ACCESSION   DATLBZ010000479 DATLBZ010000000
VERSION     DATLBZ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102705"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VMZ53_24305"
     CDS             <1..1130
                     /locus_tag="VMZ53_24305"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HUS31656.1"
                     /translation="DKKLLTTDMDAAIARPDVDVVCELIGGTTDAKRAVLRAIGAGKH
                     VVTANKALLAEHGAEVFGAAEQSGVDVYYEAAVCGGVPIIRVLREGLASDRVEALWGI
                     VNGTSNYILSTMTETRRAFGDILADAQRLGYAEADPTLDVGGGDAAHKLAILVNLCFG
                     TQVDVSKIPMQGIDVVETIDLVYAEKFGYVIKPLVIAREHGQQIEARVHPALVPANWL
                     LADVNGAKNAVYVQSYALGPSMYYGAGAGMLPTAMSVVSDMIEIGRNIFARAAGGARP
                     QRPRPASARPLLPLDDIRSKYYLRFGVVDQPGVLGQLMTILGSCSVSIEQVVQETKNT
                     NPVQVVVLTHEAREGDMRRALAEIDRLNVVKTPVRVIRIAG"
     gene            1130..1519
                     /locus_tag="VMZ53_24310"
     CDS             1130..1519
                     /locus_tag="VMZ53_24310"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HUS31657.1"
                     /translation="MGHRYAYQIIFGDTDQMGVVYYGNYLRFFEGARASYWRSLGRSY
                     KDLEAANIALPVIEAHCNYKRPAHYEDLIEVDLRVAEVRAASLRFEYTVHRGADILAD
                     GYTRHAVIGPNGRPRALPDDMRALIVG"
     gene            1519..2253
                     /locus_tag="VMZ53_24315"
     CDS             1519..2253
                     /locus_tag="VMZ53_24315"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS31658.1"
                     /translation="MRHVVLVLVVALAGCDGVFNLDKLHLPPDSAVDAPPLDASLDDG
                     LVLHLSFDGNLTDSVSGVPAACVGGANTCPKLAAGNLGSAAAFDGADDCLTANLSVVG
                     TVITVALWFNVPTDTSVSLIAKPYTGALDSWQIDTDSNRTLRFISFDTNGATGVVLPT
                     AFTVNTWVHVAITYDGASVRNLYVNGSLRANDGPSQFAIDTGPVYIGCDRDGGAPARH
                     MAGSLDDVRVYRRVLTADEIKALANL"
     gene            2267..3265
                     /gene="glpX"
                     /locus_tag="VMZ53_24320"
     CDS             2267..3265
                     /gene="glpX"
                     /locus_tag="VMZ53_24320"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HUS31659.1"
                     /translation="MQDRNLALEVVRITEAAALASARLMGKGDRKLADNVAVEAMRVA
                     FDAIDIRGTVVIGEGERDEAPMLYIGEQVGAGWKDGGGNSPKVDIAVDPLEGTNLCAT
                     GAPDAICVIAIADDGQFLNAPDTYMQKIAVGPEARGVIDLRESVTWNLQRIAKAKGKK
                     VEDCTAVVLDRERHAEIIGELRQAGARIRLIGDGDVSGAVMTANPDSGIDVLFGTGGA
                     PEGVIAAAALKCVGGELQGRLKWRSPEELARGKKMGIKDEDHIYMLDELAKGNVMFAA
                     TGVTKGYLLEGVRFYGDSAKTESIVMRSKSGTVRVISATHHFNTKPKYSWATGLEP"
     gene            complement(3290..>5564)
                     /locus_tag="VMZ53_24325"
     CDS             complement(3290..>5564)
                     /locus_tag="VMZ53_24325"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31660.1"
                     /translation="AAQLAHPNVVHIYEFGKVEHDYFIAMEFVDGVHAGQVFKQAEKE
                     KIPPTLVARIGADAAAALHYAHELRNPNGKLYGLVHRDVSPANIMISFDGVVKLCDFG
                     IAKAAAAGDQLTNPGQVKGKYAYMSPEQTIAAPLDGRSDVFSLAIVLWELLTGKYIVP
                     RGDAVAAMRAIRDGKLEAVEKINPTVPPQLAKAINWALKPKREDRATAMDLANSLEEF
                     IKGSPEIANAMQLAAWVRKRFEREGTGQVPAIPATGAVGSQSPGTQASPGTMAAPSTM
                     AGGGTGETPTPLPITPPAYPPRRVVKLMADTGEGTEIYSASDLDAEAAQTIKGGRKPP
                     AFAAETAIANDDPIAPARRIFDRSQHAAEEATLRRPQPADIGDETEIVRSAAPAPDEE
                     DSENSLLGETVMRDRRTPIPSGPTVVPTRVQSSMPLAEGTGNEGQQAPPFVGFPGTTP
                     GTGVNRPGSAPNIARPSSAGVGGVGAGGVQGSGARVMPSAGHVPPSAPRAMPDGRLPT
                     GLPTDAARQKRRRLILTIAGLGGLMLLSFMVALAASNDGPKPTPKAAPKQDGGGIAIT
                     PITIDAPLPVAPPPVDAPSVPTVPSVTLDAAIGAGEAYLEVRTIPDGGTVKVGDQSRE
                     AVVPPGDPKAKPVAQMILSAGKHHVTVELEGWQPEKREVNLEVGEHQRIEIAFTKKIT
                     KSVDRGPAMGRLSVRTTPWSDVYLGSKKLGQAPFADIELPAGTHTLTFTNPSRPTVTK
                     KVKITAGKSTKLNFNLP"
BASE COUNT          892 a         1862 c         1851 g          959 t
ORIGIN      
        1 tcgacaagaa gctgctgacc acggacatgg acgcagccat cgcgcgcccc gacgtcgacg
       61 tcgtgtgcga gctgatcggc gggaccaccg atgcgaagcg cgctgtcctg cgtgcgatcg
      121 gcgcgggcaa gcacgtcgtc accgcgaaca aggcgctgct cgccgagcac ggcgcggagg
      181 tcttcggcgc tgccgagcaa tccggcgtcg atgtctatta cgaagctgcg gtgtgcggcg
      241 gcgtgccgat cattcgcgtg ctgcgcgagg gcctcgcgag cgatcgcgtc gaagccctgt
      301 ggggcatcgt caacgggacg tcgaactaca tcctctcgac gatgacggag acgcgccgcg
      361 cattcggcga catcctcgcg gacgcccagc ggctcggcta cgcggaagcc gacccgacgc
      421 tcgacgtcgg cggtggcgat gccgcgcaca agctcgcgat cctcgtcaac ctctgctttg
      481 gcacgcaggt cgacgtctcc aagattccga tgcagggcat cgatgtcgtc gagacgatcg
      541 atctcgtcta cgcggagaag ttcggctacg tcatcaagcc gctcgtcatc gctcgcgagc
      601 atggccagca gatcgaagcg cgcgtgcatc ctgcgctcgt gcccgcaaac tggctgctcg
      661 cggacgtcaa cggcgcgaag aacgcggtgt acgtgcagag ctacgcgctc ggtccgtcga
      721 tgtactacgg cgcgggcgcg ggcatgctgc ccacggcgat gtcggtcgtc agcgacatga
      781 tcgagatcgg tcgcaacatc ttcgcgcgcg ccgctggcgg agctcgcccg caacgcccac
      841 gcccggcatc cgcccgccca ctgcttccgc tcgacgacat tcgcagcaag tactacttgc
      901 ggttcggcgt cgtcgatcag cccggcgtcc taggccagct catgacgatc ctcggctcgt
      961 gctcggtatc gatcgagcag gtcgtgcaag agacgaagaa cacgaacccg gtgcaggtcg
     1021 tcgtgctcac ccacgaagcg cgcgaaggcg acatgcgtcg cgcactcgcc gagatcgatc
     1081 gcctcaacgt cgtgaagact ccggtgcgtg tgatcaggat cgccggctaa tgggccaccg
     1141 ctacgcgtac cagatcatct tcggcgacac cgaccagatg ggcgtcgtct actacggcaa
     1201 ctacttgcgg ttcttcgagg gagcgcgcgc gtcgtactgg cgatcgctcg gtcgctcgta
     1261 caaagacctc gaagccgcga acatcgcact gcccgtaatc gaggcgcact gcaactacaa
     1321 gcggccggcg cactacgagg acctcatcga ggtcgacctg cgcgtcgccg aggtccgcgc
     1381 cgcatcgctg cgcttcgagt acaccgtcca tcgcggcgcc gacatcctcg ccgacggcta
     1441 cacgcgccac gccgtcatcg gtccgaacgg aagaccgcgt gcgctgcccg acgatatgcg
     1501 agcattgatc gtcggctgat gcggcacgtc gtcctcgtcc ttgtcgtcgc gctcgccggt
     1561 tgcgacggcg tgttcaacct cgacaagctg catctgcctc ccgacagcgc cgtcgatgcg
     1621 ccgccgctcg atgcgtcact cgacgacggg ctcgtcctgc acctgtcatt cgacgggaac
     1681 ctcactgata gcgtgagcgg tgtgcccgct gcgtgcgtcg gcggtgcaaa cacgtgcccc
     1741 aagctcgcgg cagggaacct tggctcggcc gccgcgtttg acggcgctga cgattgcctg
     1801 accgcgaatc tgtcggtcgt cgggaccgtg atcacggtgg cgctttggtt caacgtcccg
     1861 acggacacga gcgtgtcact gatcgcgaag ccatacacag gcgcgctcga tagctggcag
     1921 atcgataccg attcgaaccg cacgcttcgc ttcatctcgt tcgatacgaa cggcgccact
     1981 ggcgtcgtgc tgcctaccgc cttcaccgtc aatacgtggg tgcatgtcgc gatcacctac
     2041 gacggcgcga gcgtgcgcaa cctgtacgtc aatggctcgc tccgcgcgaa cgacggcccg
     2101 tcccagttcg ccatcgacac cggccccgtg tacatcggct gcgatcgcga cggtggcgca
     2161 cccgcacgcc acatggccgg ctccctcgac gacgttcgcg tttatcgccg tgtcctcacg
     2221 gccgacgaga tcaaggcact cgcgaacctg tgatacaccc aggcgcatgc aagaccgcaa
     2281 tctcgctctc gaagtggtcc gcatcaccga agccgctgca ctcgcctccg cacgcctcat
     2341 gggcaagggt gaccgcaagc tggcggacaa cgtcgccgtc gaagcgatgc gcgtcgcgtt
     2401 cgatgcgatc gacatccgcg gcacggtggt catcggcgag ggcgagcgcg acgaagcgcc
     2461 aatgctctac atcggcgagc aggtcggcgc cggttggaaa gacggcggcg gcaattcacc
     2521 aaaggtcgac atcgcagtcg atccgctcga aggcacgaac ctctgcgcca ccggcgcgcc
     2581 cgacgcgatc tgcgtcatcg cgatcgccga cgacggccag ttcctgaacg cgcccgacac
     2641 atacatgcaa aagatcgccg tcggccccga agcccgcggc gtgatcgatc tgcgcgagag
     2701 cgtcacctgg aacctccagc gcatcgccaa agccaaaggc aagaaggtcg aagactgcac
     2761 ggccgtcgtt ctcgaccgcg agcgccacgc ggaaatcatc ggcgagctcc gccaagccgg
     2821 cgcgcgcatc cgcctcatcg gcgacggcga cgtctccggc gcggtcatga cagccaaccc
     2881 ggactcgggc attgacgtcc tgttcgggac gggtggcgcg cccgaaggcg tgatcgcagc
     2941 ggccgccctc aagtgcgtcg gtggcgagct gcaaggccgc ctgaaatggc gcagccccga
     3001 agagctcgcg cgcggcaaga agatgggcat caaagacgaa gaccacatct acatgctcga
     3061 cgagctcgcg aaaggcaacg tcatgttcgc cgcgaccggc gtgacgaagg gctacctcct
     3121 cgagggcgtc cgcttctacg gtgacagcgc caagaccgaa tcgatcgtga tgcggtcgaa
     3181 gtcgggcacc gtccgcgtga tcagcgcgac gcaccacttc aacacgaagc cgaagtactc
     3241 gtgggccacg ggcctcgagc cctgaccgcc gtccgcaact cgcatcgcgc tacggcaggt
     3301 tgaagttcag cttcgtcgac ttgcctgcgg tgatcttcac cttcttggtc accgtcgggc
     3361 gcgaggggtt cgtgaacgtg agggtgtgcg tgcccgcggg aagctcgatg tcggcgaacg
     3421 gcgcttggcc gagcttcttc gagccgaggt agacatcgct ccatggcgtc gtgcgcacgc
     3481 tgaggcgacc catcgcgggg cctcggtcga ccgacttcgt gatcttcttc gtgaatgcga
     3541 tctcgatgcg ctggtgctcg ccgacctcga gattcacttc gcgcttttcg ggctgccagc
     3601 cttcgagctc gacggtgacg tgatgctttc ctgcggagag gatcatttgt gcgaccggct
     3661 tggctttggg atcgcccggc gggacgaccg cttcgcgcga ttggtcgccg actttgaccg
     3721 tgccgccgtc gggaatcgtg cggacttcga ggtaggcctc gccggcgccg atcgctgcgt
     3781 cgagcgttac gctcgggacg gtcgggacgg agggcgcgtc gacgggtgga ggcgcgacgg
     3841 gcagcggcgc atcgatcgtg atcggcgtga tcgcgatgcc gccaccgtct tgctttggcg
     3901 ccgccttggg cgtcggcttc gggccgtcgt tcgaggcggc aagcgcgacc atgaaggaga
     3961 gaagcatcag gccgccgagt cccgcgatcg tcaggatgag gcgacgccgt ttttggcgcg
     4021 cggcgtccgt ggggagcccc gtcggtaggc ggccgtcggg catcgcgcgt ggcgccgatg
     4081 gcggcacgtg accagcgctc ggcatcacgc gcgcgccgga gccctgaacg ccgccggcgc
     4141 cgacgccgcc gactccggcc gacgacggtc gcgcgatgtt cggcgcggag ccgggccgat
     4201 tgacgccggt gccaggcgtg gttcccggaa agccgacgaa tggcggcgcc tgctggcctt
     4261 cgttgcccgt gccttcagcg agcggcatcg acgactgcac gcgcgtcggc acgacggtgg
     4321 gcccactcgg aatcggcgtg cgccgatcgc gcatcaccgt ctcgccgagc agcgaattct
     4381 cgctgtcttc ctcgtcgggc gcgggggcgg cactgcgcac gatttccgtc tcgtcgccga
     4441 tgtccgccgg ctgcggtctg cgcagcgttg cttcttcggc ggcgtgctgc gagcgatcga
     4501 agatccggcg cgcgggcgcg atcggatcgt cattggcgat tgccgtttcg gcggcgaatg
     4561 caggcggctt gcgcccacct ttgatcgtct gcgcggcttc ggcgtcgaga tcggacgcgc
     4621 tgtagatttc ggtgccttcg cccgtgtccg ccatcagctt gacgacgcga cgtggcgggt
     4681 aggcgggcgg cgtgatcggc agcggcgtcg gggtttcgcc cgttccgccg ccggccatcg
     4741 tgctcggagc ggccatcgtt cccggcgagg cttgcgtgcc cggcgactgc gaaccgacgg
     4801 cgcccgtcgc gggaatcgct ggcacttggc ccgtgccttc gcgctcgaag cgcttgcgta
     4861 cccacgccgc gagctgcatc gcgttcgcga tttcgggcga gcccttgatg aactcttcga
     4921 gcgagttcgc gagatccatc gccgtcgcac gatcttcgcg cttgggcttg agcgcccaat
     4981 tgatcgcttt tgcgagctgc ggcggcaccg tcgggttgat cttctcgacg gcttcgagct
     5041 tgccgtcgcg gatcgcgcgc atcgcggcca cggcatcgcc gcgcggtacg atgtatttgc
     5101 cggtgagcag ctcccagagc acgatcgcga gcgagaacac atcgctgcgt ccgtcgagcg
     5161 gagcggcgat cgtctgctcc gggctcatat aggcgtactt gcctttgacc tggcccgggt
     5221 tcgtcagctg atcgcctgcg gcggcggctt tcgcgatgcc gaaatcgcag agcttcacga
     5281 cgccgtcgaa cgagatcatg atgttcgcgg gcgagacgtc gcggtggaca aggccgtaga
     5341 gcttgccgtt cggattgcgg agctcgtgcg cgtagtggag tgcggccgcg gcgtcggcac
     5401 cgatgcgtgc gacgagcgtc ggcgggatct tttccttctc cgcctgcttg aagacctggc
     5461 cggcgtgcac gccgtccacg aactccatcg caatgaagta gtcgtgctcg acctttccga
     5521 actcgtagat gtggacgacg ttgggatgcg caagctgcgc agcg
//
LOCUS       DATLBZ010000480         8103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8505660, whole genome shotgun sequence.
ACCESSION   DATLBZ010000480 DATLBZ010000000
VERSION     DATLBZ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8505660"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VMZ53_24330"
     CDS             <1..417
                     /locus_tag="VMZ53_24330"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvA C-terminal domain-containing protein"
                     /protein_id="HUS31661.1"
                     /translation="HGGGHEDANLLLLCGGHHRLLHDGLLTITGRAPGELTFARNGVH
                     LRSASYEPRPQRSGRHSHAERKHARAPSSNDGAHLGANNNAALAEQALRQLGFKAHVA
                     SRAVELASAHVGTDAPLPDLIKAALRTPMCAPTRNA"
     gene            complement(697..2460)
                     /locus_tag="VMZ53_24335"
     CDS             complement(697..2460)
                     /locus_tag="VMZ53_24335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31662.1"
                     /translation="MSDAAHSSQPPGQFYCPSCEKNFAAGDVCPDDGTRLVRLKVTDP
                     LIGKVLDGRYTIHEKLGQGGMGAVYRGSQHSVGREVAIKLVTPQLVSDHDVIKRFLRE
                     AKLASRLSHPNAVAVMDFGQTEDGLFYLVMELVTGRTLDQIIKAEKKLKPERVVRIGM
                     QVCDALEGAHALQIVHRDLKPANIMLLAQGRDLVKVLDFGLAKSLAPDQTSTTMTNAG
                     ALLGTPAFMPPELALGQSCDGRADLYSLGCILFLLGSGRLPFVSDSAHELIAMHGMQP
                     APPMAGVPPQLAEVIDRMLEKDPAKRYQSAAENREALEAAIEAHRIMTPVSGMPRVSA
                     EQSSPANPLATAVGLGVPAKTSTPGRPSASTPATASPVVSATFPTPTPTPSAQRPAHG
                     SLEMEQLVTSDTMVAQSAPPATAMTSAVSPAMSSAIAQPKKSRAALVAVLAAVLVLGG
                     VAALALSGKSSSSSSSEKPAVTAPETTPPPSVQATTPPEVKPPEAKPPEQQPAVKIDT
                     TLPEVGNVVPEVTVTKQEKTTAITKTTKVTKTTPDKMTKVTKTTPDKTTKTSPDKTKT
                     TPDKTKATSGTGSGSTMTLPF"
     gene            complement(2457..3428)
                     /locus_tag="VMZ53_24340"
     CDS             complement(2457..3428)
                     /locus_tag="VMZ53_24340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31663.1"
                     /translation="MRIVATLVAALAVFGIPQAHAEDRAALQAKGEQLAKDGKYTDAI
                     DAFKAADRIEPRAIHACLISLAYTRRELWAQAELYLSFCHQRAKVGDPLPDWVSLADQ
                     QIRDRLVAAGMVEVSIEAKPATARVTVSSFAPDEVFEPRTIHLPIGTHVIFAKAAGYT
                     DGQLTVQITDKTPKHIVIELAPVAGSAHPGEPLVGKGEVFVPEKQQPIPERHDDAPAK
                     AGTPLLIGGGAAIALGIVAHGWMGFEHGKLEEANKPPITSASEELYAAHSGRYDVARI
                     ATVALYVTGVACVGLGFYQRSHSEKATAVAAVPLPEGGAVVTVGWRR"
     gene            3518..4951
                     /locus_tag="VMZ53_24345"
     CDS             3518..4951
                     /locus_tag="VMZ53_24345"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HUS31664.1"
                     /translation="MSRSPYIGLHVNVANRRATAADIVASVQREIETGALPAGSRLPP
                     VRALEKQLGLSKNTAQAAYDELVARGLVEAREREGVFVLAASRFAAPEPVVRAPLPEF
                     VPPPLARPDFTPRGTTPLSTVFIDPELLPTERIAECARSVLRERMPAHYDAQGYPPLR
                     EAIAKRLQARGLDVEADEVVITTGSQQSLDIVARSLAVKRVAVESPVYAYAKLLFESL
                     GHELVGLHLDPFKGIDLDAWDRALAAKPSLAYLIPSFHNPTGYSYTSTELRGVLDLCT
                     KRGIAILEDDWGSDMLSDGEYRPMLRMLGGKDVLYVNSFTKKLLPSLRVGFVAAHPSL
                     VPTLVAMKRLSTLGNAWITEAVVAEFLDRGYYDTHLQQLQKALDHRYASCLAALDELM
                     PDGVRWTHPGGGPTLWLELPKRIDLVALEAHLARRGILISNTSAAYVGPAHLHGFRVA
                     YAFLPEEEMRKALTIVADAIRSDMWQK"
     gene            4955..6184
                     /locus_tag="VMZ53_24350"
     CDS             4955..6184
                     /locus_tag="VMZ53_24350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31665.1"
                     /translation="MSLRPLVGLLACAACNSAPAATSDFWGPTIEPPRGLARIAPGMS
                     VDEAKRLVPALHEPKRQGVRDELIVDSGVSDVKLTVRVEAGTVSGIIAIAQGQSVRDL
                     LTRAWGLPEIARDPLGQPEVTWASESTGWKVKLDCMERNCLVEFMPYRVLTAEFFGPH
                     VVPPGELEKLRIGMPLAQAKELAPGPVSVRAGIATKYDGVREFVAVDDKAATIRSIYL
                     NLPPAAAELVAEAWGPPTTITWVGKQVDVWPDPETGWRATLRPALGKSQDLVFENYIP
                     AVALFGDQPDTLEALPEPVLGRTLDEVKQAYKEQVTTQGKNLVITLPPTEWDRFNTRI
                     QLNIGGGRVREIQFSVPWKPNPRARDTLLDLFKAKWGQPEEVVEDSKPALLFRTDDPR
                     VEVTDDPDHGAWSISIR"
     gene            complement(6186..6392)
                     /locus_tag="VMZ53_24355"
     CDS             complement(6186..6392)
                     /locus_tag="VMZ53_24355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31666.1"
                     /translation="MSYRNDHDAALARIAALEHENAQLKRELEGIRAVRRAVFVPQRD
                     WIAVGLAGLTGLAFGLCVLATSLS"
     gene            complement(6411..6575)
                     /locus_tag="VMZ53_24360"
     CDS             complement(6411..6575)
                     /locus_tag="VMZ53_24360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31667.1"
                     /translation="MIYRQPGKPFRVEIREEIRPRLDEYPETPYISVAGLLALPAFYA
                     VIVALFRVWS"
     gene            complement(6638..6859)
                     /locus_tag="VMZ53_24365"
     CDS             complement(6638..6859)
                     /locus_tag="VMZ53_24365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31668.1"
                     /translation="MSYRNDHDAALARINALELEDARLRAENAELRAALRHAPIVAEP
                     YRVSLPTIAAVATLGVFSAVAGLVVALAL"
     gene            complement(6870..7157)
                     /locus_tag="VMZ53_24370"
     CDS             complement(6870..7157)
                     /locus_tag="VMZ53_24370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31669.1"
                     /translation="MTMYRDDHEAALARVDVLEADRARLQAENEELRTTLRDATLRSL
                     TGYVRGRDAQVTTAPARANERVNGKELAIIALGGGLGAVLLLAVALLMASP"
     gene            complement(7214..>8103)
                     /locus_tag="VMZ53_24375"
     CDS             complement(7214..>8103)
                     /locus_tag="VMZ53_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HUS31670.1"
                     /translation="REYVLRRPPFGSKVKSAHDMGREVAVLSKLAPVYSRAPRVIAYR
                     DEPDVLGAPFYLMERRRGVILRKDLPPGLSESKVRELGNHLVDALVDLHAVDYTAAGL
                     GDFGKPAGYIERQVRGWSERYVGSQTDDIPTMTSVAAWLAANMPGEGAPSLIHNDFKF
                     DNVIFDPSLDRITGVLDWEMATIGDPLMDLGGGLSYWVESTDAAVFQMPIFGVTMKPG
                     MFKRVDYAARYFERSGRRTDNIVFYYAFGLFKTAVILQQIYYRFAKGLTQDPRFAPFI
                     HVVRALGDQAQRSIDRNSL"
BASE COUNT         1336 a         2673 c         2720 g         1374 t
ORIGIN      
        1 cacggcggcg gacacgagga cgcgaacttg ctcctgcttt gtggtggcca tcatcggctg
       61 ctgcacgacg gcctgctgac catcacggga cgcgcaccgg gcgagctgac cttcgcgcgc
      121 aatggagtgc atctgcgcag cgcgagctac gagccgcgac cacagcgaag cggacgccac
      181 agccacgcgg agcgcaagca cgctcgtgca ccctcgagta acgacggcgc gcacctcggc
      241 gcgaacaaca acgctgcact cgcggagcaa gcgctgagac agctcggatt caaggcgcac
      301 gtcgcgtcgc gcgccgtcga gctagcgagt gcccacgtgg gcacagacgc gcctcttccc
      361 gacctgatca aggccgcgct acgcacgccg atgtgtgctc caacgcgcaa cgcgtagaga
      421 gccacgtgcc gacccgcgac gtacacacgc ccgctgcagt cggtgtcgag caggcttgaa
      481 agtcgccgag cgacgtcacc aacgaggagt cgaggcttct aacgaacacg tcgatctctc
      541 acgtcgctaa cctcgacgta agaggtcgtc gtggcgcgcg ggcacgacgc tcggcggcgc
      601 cacgacctcg cctcgaagta agaggtcgtc gaggcgcgcg cgggcacgac gctcggcggc
      661 gtcacgacct cgtgttagtc gagcactcgc gcttcgtcag aacggcagcg tcatcgtcga
      721 gcccgagccg gttccgctcg tcgccttcgt cttgtcgggc gtcgtcttcg tcttgtcggg
      781 ggaggtcttc gtcgtcttgt cgggcgtggt cttcgtgacc ttcgtcatct tgtcgggcgt
      841 ggtcttcgtg accttcgtgg tcttcgtgat cgccgtcgtc ttctcttgtt tcgtgacggt
      901 gacctccggg acgacgttac cgacttcggg cagcgtcgta tcgatcttca cggcgggctg
      961 ctgctcgggc ggctttgcct cgggcggctt tacctcgggc ggcgttgttg cctgcacgga
     1021 cggcggcggc gtcgtctcgg gcgcggtgac cgccggcttc tccgacgacg acgacgacga
     1081 cgacttgccg ctgagcgcga gcgcggcgac accgccgagc acgagcacag ccgcgagcac
     1141 cgcgaccaac gctgcgcgcg atttcttcgg ctgtgcgatc gctgaggaca tcgccgggga
     1201 gacggctgac gtcatcgccg tcgcgggcgg cgcggactgc gccaccatcg tgtcggacgt
     1261 gacgagctgc tccatctcga gcgagccgtg cgcgggacgc tgcgcactcg gcgtcggcgt
     1321 tggcgtaggg aatgtggcgg agacgacggg cgacgcggtt gcgggcgtcg atgcgctcgg
     1381 gcgaccgggc gtcgacgtct ttgctggaac gccgaggccg acggctgtgg ccagcgggtt
     1441 tgccggcgac gattgctcgg cgctgacgcg cggcattcct gagacgggcg tcatgatgcg
     1501 atgggcctcg atcgcggctt cgagcgcttc gcgattttcg gcagcggatt ggtaacgctt
     1561 cgccggatcc ttctcgagca tgcgatcgat gacttcggcg agctgcggcg ggacgccggc
     1621 catcggcggc gcgggctgca tcccgtgcat cgcgatcagc tcgtgggccg agtcggagac
     1681 gaacggcagg cggccggagc cgagcaggaa gaggatgcag ccgagcgaat agagatcggc
     1741 gcgaccgtcg cacgattggc cgagcgcgag ctcgggcggc atgaaggctg gcgtgccgag
     1801 cagcgcgccc gcgttcgtca tcgtcgtcga cgtttgatcg ggcgcgagcg atttcgcgag
     1861 gccgaagtcg aggactttga cgaggtcgcg gccttgcgcg agcagcatga tgttcgcggg
     1921 cttgagatcg cgatggacga tttgcagagc gtgcgcgcct tcgagcgcgt cgcatacctg
     1981 catgccgatg cggacgacgc gctcgggctt gagcttcttc tccgctttga tgatctggtc
     2041 gagcgtgcga cccgtgacga gctccatcac gagatagaac aggccgtcct cggtctggcc
     2101 gaagtccatc acggcgactg cgtttggatg cgagaggcgg ctagcgagct tggcttcgcg
     2161 gaggaaccgc ttgatgacgt cgtggtcgct gacgagctgc ggcgtcacga gcttgatcgc
     2221 gacttcgcga ccgacggagt gctgcgagcc gcggtagacc gcgcccatgc cgccttggcc
     2281 gagcttctcg tggatcgtgt aacgaccgtc gaggaccttg ccgatcagcg gatcggtcac
     2341 cttgagacga acgaggcgcg taccgtcgtc ggggcagacg tcgcctgcgg cgaagttctt
     2401 ctcgcacgac gggcagtaga actgtccggg cggctgcgag gagtgcgcgg cgtcgctcat
     2461 cggcgccacc ccacagtgac gacggcgcca ccttcgggca gcggaacggc ggcgacggct
     2521 gtcgccttct cgctgtgcga tcgctgatag aagccgaggc ccacgcacgc cacgccggtg
     2581 acgtagagcg cgaccgttgc gatgcgcgcg acgtcgtagc gacccgagtg cgccgcgtag
     2641 agctcctcgc tcgccgacgt gatcggcggc ttgttcgcct cctcgagctt tccgtgctcg
     2701 aagcccatcc agccatgcgc gacgatgccg agcgcgatcg cggcgccgcc accgatgagc
     2761 agcggcgtgc ctgccttcgc gggcgcgtca tcatggcgct cgggaatcgg ctgctgtttc
     2821 tctgggacga agacttcgcc tttgccgacg agcggctcac ctgggtgtgc gctgccggca
     2881 acgggcgcga gctcgatgac gatgtgcttc ggggtcttgt ccgtgatctg cacggtgagc
     2941 tggccgtcgg tgtagccagc ggccttcgcg aagatgacgt gcgtgccgat cggcaaatgg
     3001 atcgtgcgcg gctcgaacac ttcgtcgggc gcgaagctcg agaccgtcac gcgcgcggtc
     3061 gcgggcttcg cttcgatcga gacttcgacc atgcccgctg cgaccaagcg atcgcgaatc
     3121 tgctgatcgg cgagcgagac ccagtccgga agcgggtcac cgaccttcgc gcgctgatga
     3181 cagaacgaga ggtacagctc ggcttgcgcc catagctcgc ggcgcgtgta tgcgagcgag
     3241 atgaggcacg cgtggatcgc gcgcggctcg attcgatccg ccgctttgaa cgcgtcgatc
     3301 gcatcggtgt acttgccgtc tttggcgagt tgctcgccct tcgcttgaag ggctgcgcgg
     3361 tcctcggcat gggcttgcgg gatgccgaat accgccagtg ccgcgacgag agtcgccacg
     3421 atcctcacga cttgaagatg ctaacacgtg cctgtccctt gggccctttc ggtcctgtcc
     3481 cttccgggcg aagccacatg agcggtatga cccgaccatg agtcggtctc cctacatcgg
     3541 cctccacgtc aatgtcgcaa accgccgtgc gacggctgcc gacatcgtcg cgagcgtcca
     3601 gcgcgagatc gaaaccggcg cgcttccagc gggatcccgg cttccgcctg tgcgcgcgct
     3661 cgagaagcag ctggggctgt ccaagaatac cgcgcaggcg gcgtacgacg agctggtcgc
     3721 acgcggcctc gtcgaagcac gggagcgcga gggcgtgttc gtgctcgccg cttctcggtt
     3781 cgcagcaccc gagccggtcg tcagagcgcc gctgcccgaa ttcgtcccgc cgccgctggc
     3841 gcgtccagac ttcacgccgc gcgggacgac gccgctctcg acggtgttca tcgatccaga
     3901 gctcttgcca acagagcgca tcgcggagtg cgcgcgatcg gtgctgcgcg aacgcatgcc
     3961 ggcgcattac gacgcgcaag gctatccacc gctgcgcgaa gcgatcgcga agcggctgca
     4021 ggcgcgcggc ctcgatgtcg aggcggacga ggtcgtgatc acgacgggct cgcagcagtc
     4081 gctcgatatc gtcgcgcggt cgctcgccgt gaagcgcgtc gcggtcgagt cgccggtcta
     4141 cgcctacgcg aagctcctgt tcgagagcct aggtcacgag ctcgtcggcc tgcatctcga
     4201 tccgttcaag ggcatcgacc tcgacgcctg ggatcgcgcg ctcgccgcga agccatcact
     4261 cgcgtatctg attccgagct ttcacaaccc gacgggctac tcgtacacga gcaccgagct
     4321 gcgcggcgtg ctcgatctct gcacgaagcg cggcatcgcg atcctcgagg acgattgggg
     4381 ctccgacatg ctgtccgacg gcgagtaccg cccgatgctg cgcatgctcg gcggcaagga
     4441 cgtcctctac gtcaactcgt tcacgaagaa gctgttgccg tcgctgcgcg ttggcttcgt
     4501 cgccgcgcat ccctcactcg tgccgacgct cgtcgcgatg aagcggctct cgacgctcgg
     4561 caacgcgtgg atcacggaag ccgtcgtcgc ggagtttctc gatcgcggct actacgacac
     4621 gcatctgcaa cagctgcaga aggcgctcga tcatcgctac gcatcgtgtc tcgctgcact
     4681 cgacgagctc atgcccgatg gcgtgcgttg gacgcatccc ggtggcggtc cgacgctgtg
     4741 gctcgagctg cccaagcgaa tcgatctcgt cgcgctcgag gcgcatctcg ctcgtcgcgg
     4801 cattctcatc tcgaacacgt cggcggccta cgtggggcct gctcacttgc acggtttccg
     4861 tgtcgcgtat gcgtttctgc ccgaggaaga aatgcgcaag gcactaacga tcgttgccga
     4921 cgcaatccgc tctgacatgt ggcaaaaata gggcgtgagc ctcaggccgc tcgtcggact
     4981 cctcgcatgc gccgcgtgta acagcgcgcc cgcagccacg agcgatttct ggggacccac
     5041 catcgagcca cctcgtggcc tcgcgcgcat cgcgccgggc atgagcgtcg acgaagcaaa
     5101 gcgcctcgtt cccgcgctcc acgagccgaa acgccaaggc gtgcgcgacg agctcatcgt
     5161 cgactccggc gtcagcgacg tgaagctcac cgtgcgcgtc gaagccggca ccgtcagcgg
     5221 catcatcgcg atcgcgcaag gccaaagcgt tcgcgatctg ctgacgcgcg cctggggcct
     5281 tcccgaaatc gcgcgcgatc cactcggcca gcccgaagtc acatgggcca gcgagtcgac
     5341 gggctggaag gtcaagctcg actgcatgga gcgcaactgc ctcgtcgagt tcatgccgta
     5401 ccgcgtgctc accgccgagt tcttcggccc gcacgtcgtg ccgccaggcg agctcgagaa
     5461 gctgcgcatc ggcatgccgc tcgcgcaagc gaaggagctc gcgccgggtc cggtctccgt
     5521 ccgcgcgggc atcgcaacga agtacgacgg cgttcgcgag ttcgtcgccg tcgacgacaa
     5581 ggccgcgacg attcgctcga tctatctcaa cctcccgccg gctgcggccg agctcgtcgc
     5641 cgaagcgtgg ggcccaccga caacgatcac gtgggtcggc aagcaagtcg acgtgtggcc
     5701 cgatcccgaa acgggttggc gcgccacgct gcgtcccgcg ctcggcaagt cgcaggatct
     5761 cgtgttcgag aactacattc cagccgtcgc cctcttcggc gatcagcccg acacgctcga
     5821 agcgctgccc gagccggtcc tcggccgcac gctcgacgag gtcaagcaag cctacaaaga
     5881 gcaagtcacg acgcagggca agaacctcgt catcacgctg ccgccgacgg aatgggaccg
     5941 gttcaacacg cgtatccagc tcaacatcgg cggtggccgc gttcgcgaga tccagttctc
     6001 cgtgccgtgg aagccgaacc ctcgcgcgcg cgacacgctt ctcgacctgt tcaaggcgaa
     6061 gtggggccag cccgaggaag tcgtcgaaga ctccaagccc gcgctgctct tccgcaccga
     6121 cgatccgcgc gtcgaagtga ccgacgatcc agaccacggt gcgtggtcga tctcgattcg
     6181 ctagctcacg aaagcgaagt cgcgagcaca cacaggccga acgcgagccc ggtgagtccc
     6241 gcgagcccga cggcgatcca atctcgctgc ggcacgaaga ccgcgcgtcg cacggcgcga
     6301 attccttcga gctcgcgctt cagctgtgcg ttctcgtgct cgagtgcggc gatgcgcgcg
     6361 agcgcagcat catgatcgtt gcgatacgac atgcgagctc ctctcgaagt tcaggaccaa
     6421 acgcgaaaca gcgccacgat gaccgcgtag aacgcgggca acgcgagcaa gccagcgacg
     6481 ctgatgtacg gcgtctcggg gtactcatcg aggcgcggcc ggatctcttc gcgaatctcg
     6541 acgcgaaacg gttttccggg ttggcggtaa atcatggcgc ctcctacagg gtcgcacgcg
     6601 ccgggcgaga agcaagcgcg cacgacgcgt tgaatcgtta gagcgctagc gcgacgacga
     6661 gcccggccac cgcggagaac actccgagcg tcgcgaccgc ggcgatcgtc gggagcgata
     6721 cccggtacgg ctcggccacg atcggcgcgt ggcgcagcgc ggcgcggagc tcggcgttct
     6781 cggcgcgcaa gcgggcatcc tcgagctcga gcgcgttgat gcgagcgagt gcagcgtcgt
     6841 gatcgttgcg atagctcata tgtgcctcct cagggcgatg ccatgagcag cgcgaccgcg
     6901 agcagcaaca cggctccgag gcctcccccg agcgcgatga tcgcgagctc cttgccgttg
     6961 acgcgttcgt tcgctcgtgc cggagccgtg gtgacctgcg catcgcggcc tcgcacgtat
     7021 cccgtcagcg agcgcagcgt ggcgtcgcgc agcgtcgtgc gcagctcctc gttctcggct
     7081 tgcagcctcg cgcggtcggc ctcgagcaca tcgactcgtg cgagcgcggc ttcgtgatcg
     7141 tcccggtaca tcgtcattga ttcgatgcac tcaagctgtg catcggtctc gcgctgtcac
     7201 gcgaaacacg acctcacaag gagttacgat cgatgctgcg ctgcgcctga tcgccaagtg
     7261 cgcgaaccac gtggatgaac ggcgcaaagc gcggatcctg cgtgaggcct ttggcgaacc
     7321 ggtaatagat ctgctgcagg atgacggccg tcttgaacag gccgaaggcg taatagaaga
     7381 cgatgttgtc cgttcggcgc cccgagcgtt cgaagtaacg ggccgcgtag tcgacgcgct
     7441 tgaacatgcc cggcttcatc gtcacgccga agatcggcat ctggaagacg gccgcgtcgg
     7501 tcgactcgac ccagtacgag aggccaccgc cgagatccat gagcgggtcg ccgatcgtcg
     7561 ccatctccca atcgagcaca cccgtgatgc gatcgagcga cggatcgaag atcacgttgt
     7621 cgaacttgaa gtcgttgtga atcagagagg gcgcgccctc tcccggcatg ttcgcggcga
     7681 gccatgcagc gacggaggtc atcgtcggaa tgtcgtcggt ctgcgagccg acgtagcgct
     7741 cgctccagcc gcgcacttgg cgctcgatgt agccggcggg cttgccgaag tcgccgaggc
     7801 ccgcggcggt gtagtcgacc gcatgcagat caacgagcgc gtcgacgagg tgattgccga
     7861 gctcgcggac ttttgattcg gagaggccgg gtggcaggtc cttgcgaagg atcacgccgc
     7921 ggcggcgctc catcagatag aacggcgcac cgagcacgtc gggctcgtcg cggtaggcga
     7981 tcacgcgagg tgcgcgcgag tacacgggcg ccagcttgga gagcacggcg acctcgcgac
     8041 ccatgtcgtg cgcgctcttc accttgctgc cgaacggcgg gcggcggagc acgtactcgc
     8101 gct
//
LOCUS       DATLBZ010000481         6875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9558086, whole genome shotgun sequence.
ACCESSION   DATLBZ010000481 DATLBZ010000000
VERSION     DATLBZ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9558086"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VMZ53_24380"
     CDS             <1..657
                     /locus_tag="VMZ53_24380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HUS31671.1"
                     /translation="HGDEHHYLSRVRRARVGDEIEIVDGEGKRARATITAMRESETVL
                     AVGDVHEVEDKLPRVRVLVPWIKGDRMDVCLEKLVEVGADEIVVWPAARAVVKLEGER
                     RATRVAHHQAALQAAARQCGRAAVPVISAVDSLGAAVAALPVRGGRFVLDPMADRAKL
                     VPVDEVTLCSGPEGGLAPAELDALAAAGFVGVGLGPRVMRAETAPVIAVALIRAATLS
                     "
     gene            670..1905
                     /locus_tag="VMZ53_24385"
     CDS             670..1905
                     /locus_tag="VMZ53_24385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HUS31672.1"
                     /translation="MTASALRQLSEQLLSGSVSIDQAQPMAPRFLAEQVAARSYFVSS
                     FANVSAFDTDDGLVLVDTGSFILAEPTRARLRSVTKRPVSTAVWTHGHVDHCFGVDLY
                     ERDSGRRVKVIAHEAVSRRFERYKLTREWNQAINQRQFQTTAVFPGEFREPDETFDKT
                     LTVVVGGRRFELKHALGETDDHTWVWVPDAGVICTGDLFIWASPNCGNPQKVQRFPRE
                     WAAALRDMDKRGAEVLLPGHGVPIWGAAAVHQALDETATFLESLVEQVVALMNTGARL
                     DEVLARVRIREDLLARPYLNPIYDEPEFIIRNLYRLYGGWWDGNPAHLKPPREGELAA
                     EVAALAGSAMKLADRAGQLAREGQHAVACELVELAFRAAPEDALIAKIRSEVYAQRAK
                     EERSLMARGVFSAAAGSKS"
     gene            2015..2500
                     /locus_tag="VMZ53_24390"
     CDS             2015..2500
                     /locus_tag="VMZ53_24390"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HUS31673.1"
                     /translation="MGRREASSVFVVGFWKRFAAAFVDLMVVVPVALLITYIVSKVSG
                     VHLPAKNLKLTDIDMWIDLALASNPAIVMGIVMFLAIGMVYLFVFHMTLGRTLGMRLL
                     KMKVIDVYGDPPSPARCAARCAGYLASIATLFLGFLWIGFDSEKRGLQDWIAGTYVIR
                     A"
     gene            2502..5075
                     /locus_tag="VMZ53_24395"
     CDS             2502..5075
                     /locus_tag="VMZ53_24395"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31674.1"
                     /translation="MPARLSSLLVRDGLVGVKRMEKAFQRQVIYGGSLDTILLEMNLV
                     PEERLTQYLALASGLPPAAKDEAARLDSAALALFSSEISAQFRAVPLGFETSGDAVRV
                     LVCSPLEISELEDLADLIDRPLQPLITPEYRWHLAYAKAYRVDAPARFTTLARQLEVE
                     AAGGPAPVGRSKSVIVDEAGPATQPLTQAPPPLAATVPAPPVLPKAVPPPTRVQRPTP
                     PPVPPQRPTPPPLPAAVPELPPLQDATDKIVFEPPPSLTEHKGELRSRTSTMAGMAVP
                     RARQDTDPGAGPLPRQPAVTAAVVTPSTGIPKAVADSATGRSSVKRGMMEPIATTGRD
                     SPLAIVRARELLATADDRDTVFLTLLRAARARARYAGLLTVQGGAAIGRVALAETGID
                     IAAIQKVLIPLDLMSPFRTVVNNQQPHIGPIVGGEQTIDQMVMRMGGTLPPSALIMPV
                     VLRERVVALVVAHRVHSDLKLIDVTELLPLAAGAADALGRLIVKHKSAGYRAPAQAAP
                     RVPIEGDRIDTKKIVRKDAEWRTPAPAERPSVPDIETEELSITAEPPRLINEVLDEIE
                     NAKEGQAEDAITDAVERAVESVGALAKRFPGKLRVDRFAVSGRPLRAAQYGGLLELTV
                     RLGSVASELLIEQMSSGERDTRFYATVCAAELRPRNAIFVLVERLFDQDFGVRDIAKE
                     ALAGYPLAELTQALAKARRAIHSDEPETVAAATSAIVQLGDVEAVSDLIGAIERGDRG
                     AEHVRKALVSLTAQDFGLSEKKWRKWFDGARRRHRIEWLIEGLLHKEDAIRELAINDL
                     RRLTGEYFGYHHDLPKKEREVAAERWSSWWRETGQRRFVQREDERHRPTAVIPARHEP
                     S"
     gene            complement(5072..5638)
                     /locus_tag="VMZ53_24400"
     CDS             complement(5072..5638)
                     /locus_tag="VMZ53_24400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31675.1"
                     /translation="MAVAVALWSLLQMLGMGIGLSSIDLGSEGSLRHVRVGNSVWSML
                     APLIALAVGGIVAGKLASTFDQRVGATHGFVVGALSSVIGLILTVSLVSMLAGSSVSS
                     ATSRFLTTDNVLVTPSMRNAERAAAAKDMGKILFGAGLSLLVGIGAAVAGGALAVSPL
                     NRRRKHDTAEVPVVPPPAAPPIDAPHVA"
     gene            complement(5783..6352)
                     /locus_tag="VMZ53_24405"
     CDS             complement(5783..6352)
                     /locus_tag="VMZ53_24405"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HUS31676.1"
                     /translation="MRLLLLSLVPVAGLAGVVGVRHFEPQPEAELAHVSRAQEIRSIA
                     IDGRGLPMASLRELLSTHAGEQVDTAKLTHDREALEQELVDRGYYAAHVTNAQVSYDA
                     NGGAFITFRVEQGPQFRVRSVIVTGATPKDAGVLAFEAGEIATADRIQLARATLADRL
                     ESRGKHLTVAANVRADDEAAVVDIELSAH"
     gene            complement(6483..>6875)
                     /locus_tag="VMZ53_24410"
     CDS             complement(6483..>6875)
                     /locus_tag="VMZ53_24410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31677.1"
                     /translation="ADGLDQRGNVPTGRYRFHVEGSGWTLDSAPFTVVEGGLSATATR
                     SNGQIHVAVKWHAPKGWRLMDMALMSNQPIPVRNKRVLIELHNSGVLSGTTPNTDANG
                     NVDVPDNSAATFVRVGDPYGNSFDATLP"
BASE COUNT         1146 a         2287 c         2333 g         1109 t
ORIGIN      
        1 cacggcgacg aacatcatta tctgtcgcgc gtgcggcggg cacgtgtcgg cgacgagatc
       61 gaaatcgtcg acggcgaagg caagcgagcg cgcgcgacca tcaccgcgat gcgcgagagc
      121 gagacggtct tggccgtcgg cgacgtgcac gaggtcgagg acaagttgcc gcgcgtgcgc
      181 gtgctcgtgc catggatcaa gggcgatcgc atggacgtgt gcctcgagaa gctcgtcgag
      241 gtcggcgccg acgagatcgt cgtgtggccg gcggcgcgtg cggtcgtgaa gctcgagggc
      301 gagcggcgag cgacgcgcgt cgcgcatcat caagcggcat tgcaggccgc ggcgaggcag
      361 tgtgggcggg cggctgtgcc ggtgatcagt gcggtggatt ccttgggcgc ggcggtggcg
      421 gcgttgccgg tgcgtggcgg aaggttcgtg ctcgatccga tggccgatcg cgcgaagctc
      481 gttcccgtcg acgaagtgac attgtgcagc gggcccgagg gcgggctggc acccgcggag
      541 ctcgatgcgt tagcggcggc gggatttgtc ggcgtcgggc ttggaccgcg cgtcatgcgt
      601 gcggaaaccg cgccggtcat tgcggtggca ttgatccgcg ccgcgacgct cagctgatat
      661 ggtcgcggca tgaccgcgag cgcgctgcgc cagctctccg agcagttgct gagcggcagt
      721 gtgtcgatcg atcaggcgca gccgatggcg ccgcggtttc tggccgagca agtggccgcg
      781 cgcagctact tcgtctcgag ctttgcgaac gtcagcgcgt tcgacaccga cgacgggctg
      841 gtcctcgtcg ataccggcag cttcattctc gcggagccga cgcgcgcgcg gttgcggagc
      901 gtgacgaagc ggccggtgag caccgcggtg tggacgcacg gccacgtcga tcattgcttt
      961 ggcgtcgact tgtacgagcg cgactcgggc cgccgcgtga aagtcatcgc gcacgaagcg
     1021 gtgagccggc gattcgagcg ctacaagctg acgcgcgagt ggaaccaggc gatcaaccag
     1081 cgccagtttc agacaactgc tgtgttcccg ggcgagttcc gcgagcccga cgagacgttc
     1141 gacaagacct tgaccgttgt cgtcggcggg cgccgcttcg agctgaagca cgcccttgga
     1201 gagaccgacg atcacacgtg ggtctgggtg ccggatgccg gcgtcatctg cacgggcgac
     1261 ctgtttatct gggcgtcgcc gaactgcggc aatccgcaga aggtgcagcg cttcccgcga
     1321 gagtgggccg ccgccctgcg cgacatggac aagcgcggcg ccgaggtgct gctgcccggg
     1381 catggcgtgc cgatttgggg tgccgcggcc gtgcatcagg cgctcgacga gaccgcgacg
     1441 tttctcgagt cgctcgtcga gcaagtcgtc gcgctcatga atacgggcgc gcgtctcgac
     1501 gaggtgctgg cgcgcgtgcg catccgcgaa gatctgctgg cgcggccgta tctcaatccg
     1561 atttatgacg agccggagtt catcatccgc aacctctacc ggctgtacgg cggatggtgg
     1621 gacggcaatc cggcgcactt gaagccgccg cgcgagggcg agctcgcggc cgaggtggcg
     1681 gcactggcgg gaagcgccat gaagctcgcg gatcgggccg gccagctggc acgcgagggg
     1741 cagcatgccg tcgcttgcga gctggtcgag ctggcctttc gggccgcccc cgaggacgcc
     1801 ctgatcgcga agattcgctc cgaggtctat gcccaacggg ccaaggaaga gcgctcgctg
     1861 atggcgaggg gcgttttctc ggccgccgcc gggtccaagt cctgaagagt atttgcctcc
     1921 ggcctctgcg gcaccgtata aaacatcgat gtcggagccc tcgctgctag tggacgagcc
     1981 cgggacgctc cctgccccga caattcagcg ttccgtggga cgacgcgaag ccagctcggt
     2041 cttcgtggtg ggattctgga agcggttcgc cgcggcgttc gtcgacctga tggtcgtggt
     2101 gccggtcgcg ctgctcatca catacatcgt cagcaaggtt tcgggcgttc acctgcccgc
     2161 gaagaacttg aagctcaccg acatcgacat gtggatcgac ctcgcgctcg cgagcaatcc
     2221 ggcgatcgtc atgggcatcg tgatgttcct cgcgatcggt atggtctatc tgttcgtctt
     2281 ccacatgacg ctcggccgta cgctcggcat gcgcctgctc aagatgaaag tcatcgacgt
     2341 gtacggcgat ccgccgtcac ccgctcgctg cgctgcgcgt tgcgctggct acctcgcgag
     2401 catcgccacg ttgttcctcg gattcctctg gatcgggttc gacagcgaga agcgtgggct
     2461 gcaggactgg atcgcgggga cctacgtgat tcgagcctga gatgccggcg cgtctgagct
     2521 cgctcctcgt gcgtgacggc ctcgtcggcg tcaagcgcat ggagaaagcg ttccagcgcc
     2581 aggtcatcta cggcggcagt ctcgacacga ttctcctcga gatgaacctc gtccccgagg
     2641 agcggttgac gcagtacctc gcgctcgcat cgggcttgcc tcccgcggcg aaagacgaag
     2701 cggcgcgtct cgattccgct gcgctcgcgc tgttctcgag cgagatctcc gcgcagtttc
     2761 gtgcggtgcc gctcgggttc gagacaagcg gcgacgcggt gcgcgtgctc gtgtgctcgc
     2821 cactcgagat cagcgagctc gaagatctcg cggacttgat cgatcgtccg ctgcagccgc
     2881 tcatcacgcc cgagtatcgc tggcacctcg cgtatgcgaa ggcgtatcgc gtcgacgcgc
     2941 ccgcgcggtt cacgacgctg gcgcgtcagc tcgaggtcga ggcagcaggt ggaccagcac
     3001 cggtcggtcg ctcgaagagc gtgatcgtcg acgaagccgg tcccgcgacg cagcctctca
     3061 cgcaagctcc gccgccgctc gccgcgacgg tccctgcccc gcccgtgttg ccgaaggcag
     3121 tgccgccgcc gacccgggtg cagcgtccga cgccgccgcc ggttccgccg cagcgaccga
     3181 cgccgccgcc actacctgcc gcggtccccg agctgccacc gctgcaggac gcgacggaca
     3241 agatcgtgtt cgagccgccg ccgtcgctca cggagcacaa gggcgagctg cgcagtcgaa
     3301 cgtcgacgat ggcgggaatg gcggtgcctc gcgcgcggca agacacggat cccggtgcag
     3361 gtcctttgcc tcgccagcca gcggtcaccg ccgctgtcgt cacgccctcg acgggaattc
     3421 cgaaagccgt cgccgattcc gcaacgggac ggtcgagcgt gaagcgcggg atgatggaac
     3481 cgatcgcgac gacggggcgc gacagcccgc tcgcgatcgt gcgtgcgcga gagctgctcg
     3541 cgaccgctga tgatcgcgac acggtgtttc tcacgctgct gcgtgcggct cgcgcgcgtg
     3601 cgcgctacgc gggcctgctc accgtgcaag gtggcgcagc gatcggccgc gtcgcgctcg
     3661 ccgagaccgg tatcgacatc gcggcgatcc agaaggtgct catcccgctc gacctcatgt
     3721 cgccgttccg tacggtcgtg aataaccagc agccgcacat cggcccgatc gtcggtggcg
     3781 agcagacgat cgatcagatg gtgatgcgca tgggcggaac actgcctcca tcggcgctga
     3841 tcatgcccgt cgtgttgcgt gaacgtgtgg tcgcgctcgt cgtcgcgcat cgcgtgcatt
     3901 cggatctcaa gctcatcgac gtcaccgagc tgttgccact cgccgctggc gcagcggatg
     3961 cgctcggtcg cctgatcgtg aagcacaagt cggcgggtta tcgcgcaccg gcgcaagctg
     4021 caccgcgcgt tccgatcgaa ggcgatcgca tcgacacgaa gaagatcgtc cgcaaggatg
     4081 ccgagtggcg aacgccggcg cccgccgagc ggccgagcgt gccggacatc gagaccgaag
     4141 agctgtcgat caccgccgag ccgccgcgct tgatcaacga ggtcctcgac gagatcgaga
     4201 acgccaaaga gggccaagcc gaagacgcga tcacagacgc cgtcgagcgc gcagttgaat
     4261 ccgtcggcgc actggcaaag cgcttcccgg gaaagctccg cgtcgatcgc ttcgcggtgt
     4321 cgggccggcc gctgcgcgcg gcacaatacg gcggcttgct cgagctgacg gttcgcctag
     4381 gctccgtcgc atcggagctg ctgatcgagc agatgagctc cggtgagcgc gatactcgct
     4441 tctacgcgac ggtgtgcgcc gccgagcttc gccctcgcaa cgcgatcttc gtcctcgtcg
     4501 agcgactctt cgaccaagat ttcggcgttc gcgacatcgc gaaagaagcg ctcgccggct
     4561 atccactcgc agagctcacg caggcactcg ccaaagcgcg ccgcgcgatt cactccgacg
     4621 agccagagac cgtggcggcg gcgacctcag cgatcgtcca gctcggcgat gtcgaggccg
     4681 tctccgatct catcggcgcg atcgagcgtg gcgaccgcgg cgcggagcac gttcgcaaag
     4741 cgctcgtctc tctcaccgcg caggacttcg gtctctccga gaagaagtgg cgcaagtggt
     4801 tcgacggcgc gcgacgccgt caccgcatcg agtggctcat cgaaggcctc ctccataaag
     4861 aggacgcgat tcgcgagctc gcgatcaacg acctgcgccg cctgaccggc gagtacttcg
     4921 gctaccacca cgatctcccg aagaaggagc gcgaggtcgc cgccgagcgc tggtcgagct
     4981 ggtggcgcga gaccgggcag cgacggttcg tgcagcgcga agacgaacgt caccgcccga
     5041 ccgccgtgat tccggcccgc cacgaaccga gttaagcgac gtgcggcgcg tcgatcggcg
     5101 gcgcagccgg cggcggaacg accggcactt ccgcggtgtc atgcttgcgc cgacgattca
     5161 gcggcgacac ggcgagagca ccgcccgcga cggcggcacc gatgccgacg agcagcgaga
     5221 gacccgcgcc gaacaagatc ttgcccatgt ccttcgcagc cgcggcgcgc tccgcgttgc
     5281 gcatgctcgg cgtgacgagc acgttgtccg tcgtgaggaa ccggctcgtc gccgacgaca
     5341 ccgagctgcc ggcgagcatc gacacgagcg acaccgtcag gataagcccg atcaccgacg
     5401 acagcgcgcc gacgacaaag ccgtgcgtag cgccaacgcg ctgatcgaac gtcgacgcga
     5461 gcttgccggc aacgatgccg ccgaccgcga gcgcgatcag cggagcgagc atcgaccaca
     5521 cgctgttgcc gacacgaacg tgccgcagcg agccttcact accgagatcg atcgaagaca
     5581 ggccgatgcc catgccgagc atctgcaaca gagaccacag cgcaacggcg accgccacgc
     5641 ccgcaaagac cgcgctccac cgcaaagccg gttggcgccg atcgctaatc agctcacgag
     5701 tataaggctt catgatcgaa gctacccagt gattcgctct cggcaaatga gcgaggtcgc
     5761 cctccgcgat cgcccgagtg cgtcagtgcg ccgagagctc gatgtccacg acagcggcct
     5821 cgtcgtccgc acgcacgttc gccgcgaccg tgaggtgctt gccacgtgac tcgagtcgat
     5881 cggcgagcgt cgcgcgcgcg agctgaatgc gatccgccgt cgcgatctcg cccgcctcga
     5941 atgcgagcac gccagcatct ttcggagtcg cgccggtgac gatcaccgaa cgcacgcgga
     6001 actgcgggcc ctgctcgacg cggaacgtga tgaaggcgcc gccgttcgcg tcgtagctga
     6061 cctgcgcgtt ggtcacgtgc gcggcgtagt aaccgcgatc gacgagctct tgctcgagcg
     6121 cctcgcgatc gtgcgtgagc ttcgcggtgt ccacctgctc gccggcgtgc gtcgagagca
     6181 gctcgcgcag gctcgccatc ggcagcccgc gaccatcgat cgcgatgctc cggatctcct
     6241 gcgcgcggct gacgtgcgcg agctccgcct ccggctgcgg ctcgaaatga cgtaccccga
     6301 ccactccggc gaggccggca acgggtacga gactgagcaa caagaggcgc acgtcccgag
     6361 ctagtgcaac gcctcggcca ggcctaatcg cccgaaatca tggagctccg ccgaacgcag
     6421 ccgtggacag cacttcacgc tccgaggtgc cgcgtatcca cgcgacggaa gtggtcttac
     6481 cgctacggga gggtcgcgtc gaacgagttg ccgtaggggt cgccgacccg gacgaacgtc
     6541 gcggcgctgt tgtccggtac gtcgacattg ccgttggcgt ccgtgttcgg cgtggtcccc
     6601 gagagcacgc cggagttatg cagctcgatc aacacgcgtt tgttccgcac cggaatcggt
     6661 tggttcgaca tcagcgccat atccatcagg cgccaaccct tcggcgcgtg ccacttcacg
     6721 gcgacgtgaa tctgaccatt cgagcgcgtc gcagttgcgg agaggccacc ttcgacgacc
     6781 gtgaacggcg cgctgtcgag cgtccaaccg ctaccttcga cgtggaaccg atagcgaccc
     6841 gtgggcacat tgccgcgttg atcgagaccg tcggc
//
LOCUS       DATLBZ010000482         4555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9559618, whole genome shotgun sequence.
ACCESSION   DATLBZ010000482 DATLBZ010000000
VERSION     DATLBZ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9559618"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1898)
                     /locus_tag="VMZ53_24415"
     CDS             complement(156..1898)
                     /locus_tag="VMZ53_24415"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HUS31678.1"
                     /translation="MSLHSPHRAAAEAWLAEDPDSKTAAELSTLIDRANAGDPAADRE
                     LREAFTGQLEFGTAGLRGVLGPGPQRMNRVLVRKVTAGLAAYLRETVKDSPQRGVLIG
                     HDSRHNSRIFAEDTARVLAGAGIKVYLAHRPWPTPTTAWGVTATNAAAGVMVTASHNP
                     PEYNGYKVYWGNGAQIVPPHDKGIAESIARTEKLGRSDQLPMPELAAARAAGLIVDLD
                     ESMHDRYLADVVALRAQPQLDGTSLIVAYTPLHGVGAPSVEPAMQRAGFPRIHTEPSQ
                     REGDPDFPTVAFPNPEEKGAMDRVLELARRSNADLVLANDPDADRLCVAVPDDGGYRV
                     LTGDQVGALLADYLLEVSPKDKRMVATTIVSSQLLSYLAKQAGADYRETLTGFKWIGN
                     AAMDYERDNAAAKGRFVMGYEEALGYSVGPLVRDKDGVSACVIFAELTAWNRARGKTV
                     LDHLDDIYRRVGLFVTEQVSLTKKGAEGIAEIKAAMSRFRANPPKEVGPHAIEQVVDL
                     SKGEGGLPPSDVLVFKLAGGRRIIMRPSGTEPKLKSYYEVRVPVGSGESIADARARGM
                     SELATLRDAHQSMI"
     gene            complement(1951..2562)
                     /locus_tag="VMZ53_24420"
     CDS             complement(1951..2562)
                     /locus_tag="VMZ53_24420"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS31679.1"
                     /translation="MKRLLILLLVLFAGTPAFAADAKTGAGTTAVKSANDKISALLKQ
                     KPAAGSKEEKELAGKVTTSVRDFLDIDELGKRAMADQWSKLTAAQQTEFLTTLRALIE
                     DNYIRGLRANLSYTVDYTGESADKDGNTVVTTTINTKRKNRTLKISVDYILKKQGDKL
                     KAWDVKTDGVGLVENYKTQFNKIIAKEGFSGLIARMKKKQSAT"
     gene            complement(2567..3889)
                     /locus_tag="VMZ53_24425"
     CDS             complement(2567..3889)
                     /locus_tag="VMZ53_24425"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUS31680.1"
                     /translation="MRLLVIALLLVPTIASAEPKKLTLDEVIAKAAANPKVQMAISDK
                     DVAESRIHEAYAAVLPRVKATIYGTVSPEIQCDNADCTRTSPQNFAFKFSGFFGGASL
                     EFTQVLYTFGKASHGLAAARAGVSAQKSLADEAAGDAAVDAARAYWGLKLARELGAML
                     EDGIEQIEGAQKGFEDRKDVTIEDRQRVAVLLAEAKVQRAEAAQAEAQALAGLRAITA
                     TPDADIDEDELLAVEKPVPEAADLANGATKRPQLLAAKSGAVAANELASFQAAYYFPD
                     LSLVGSAVISRAQGADDPPSAYANDPYNRQGLSLLLALQWSVEPWTVRARTERLRAEA
                     RKATAQAALAQIGATYDAETAIAEARSAKGKVDAANEGEKAARTWLASVLQNQAVGTA
                     EAKDLADAYIAWFQMKARWAQSVFQWNVAVVRLGRATGEFHAGPARPR"
     gene            complement(3931..>4555)
                     /locus_tag="VMZ53_24430"
     CDS             complement(3931..>4555)
                     /locus_tag="VMZ53_24430"
                     /inference="COORDINATES: protein motif:HMM:NF019109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1517 domain-containing protein"
                     /protein_id="HUS31681.1"
                     /translation="IQNALKWIAQTSNTSTKTGRAEMLGKVSLLLRNSRDAWVYGGAC
                     NERMGPLSNIKAIYDRHVDDARTRFSRETITNVDGKKSSQHAGDISRRSDEGEGLILV
                     TIVIAARSELFTVERIGTGEDLRNAIESAAFLGDQLVAVDVIWMPSEDDDRMSSMELE
                     ARYPAPDLIPIQGALVGKTFCDHCAGPHPAELVSCPHCGAPARDRAA"
BASE COUNT          695 a         1463 c         1525 g          872 t
ORIGIN      
        1 cgcaacttcc gcaacctgcg caaccgcgag ggacggagca accgagcaag cgcgtggaat
       61 gacgagcgtg atgaacgtgg atgagggcgc ggttgcggaa gttgcgtccg tcccttatcg
      121 gaatacccag gggtgtccgc cggctggaca gagcgtcaga tcatcgactg gtgagcgtcg
      181 cgcagcgtcg cgagctctga catgccgcgg gcgcgagcgt cagcgatcga ttcgccggag
      241 ccgaccggga cgcgcacttc gtagtagctc ttgagcttcg gctccgtacc cgacggccgc
      301 atgatgatgc ggcggccacc ggcgagcttg aagacgagga cgtcggatgg cggcaggccg
      361 ccttcgcctt tggagaggtc gacgacttgc tcgatcgcgt gcgggccgac ttctttgggc
      421 gggttcgcgc ggaagcgaga catggccgct ttgatttcgg cgatgccctc ggcgcccttc
      481 ttcgtcagcg agacttgctc cgttacgaag aggccgacgc ggcgatagat atcgtcgagg
      541 tggtcgagga ctgttttgcc gcgcgcacgg ttccacgcgg tgagctcggc gaagatgacg
      601 caggcggaga cgccatcttt gtcgcgcacg agtgggccga ccgagtagcc gagcgcctct
      661 tcgtagccca tcacgaagcg gcctttggcg gcggcgttgt cgcgctcgta gtccatcgcc
      721 gcgttgccga tccacttgaa gcccgtgagg gtttcgcggt agtcggcgcc ggcttgtttt
      781 gcgaggtagg agagcagctg cgacgagacg atcgtcgtcg cgaccatgcg cttgtctttg
      841 gggctgactt cgaggaggta gtcagcgagc agcgcgccga cttgatcgcc ggtgagcacg
      901 cggtagccac cgtcgtcggg gacggctacg cagaggcggt cagcgtcggg atcgtttgcg
      961 agcacgaggt cggcgttgga gcggcgcgcc agctcgagca cgcgatccat cgcgcctttt
     1021 tcttccgggt tcggaaacgc gaccgtcgga aagtcggggt cgccttcgcg ctggctcggt
     1081 tcggtgtgga tgcgcgggaa gcctgcgcgc tgcatcgcgg gctcgacgga cggcgcgccg
     1141 acaccgtgca gcggcgtgta cgccacgatc agcgacgtgc cgtcgagctg cggttgggcg
     1201 cgaagcgcga cgacgtcagc gaggtagcgg tcgtgcatcg actcgtcgag atcgacgatc
     1261 aggccagcgg cgcgtgcggc ggcgagctcc ggcatcggca gctgatcact gcggcccagc
     1321 ttctcggtgc gcgcgatcga ttcggcgatg cccttgtcgt gtggcgggac gatttgcgcg
     1381 ccgttgcccc agtagacctt gtagccgttg tactcgggcg ggttgtgact cgcggtgacc
     1441 atcacgccgg cggctgcgtt cgtcgcggtg acgccccacg cggtggtcgg tgtcggccac
     1501 gggcggtggg cgaggtacac cttgatgccg gcgccggcga gcacgcgcgc ggtgtcctcc
     1561 gcgaagatgc gcgagttgtg gcgcgagtcg tgaccgatga ggactccacg ctgtggcgag
     1621 tctttgaccg tttcgcgcag gtacgcggcc aggccggcgg tcaccttgcg gacgagcacg
     1681 cgattcatcc gctgcggtcc gggaccgagc acgccacgca ggcctgcggt gccgaactcg
     1741 agctggccag tgaaggcttc gcgcagctcg cgatcggcgg ccgggtcacc cgcgttcgct
     1801 cgatcgatca gcgtcgagag ctcggcggcg gttttcgaat cagggtcttc ggccagccac
     1861 gcttcggcgg cggcgcgatg cggtgagtgc agcgacacgg gctgacgata accaaaaagc
     1921 ggtcagccgg tagcttttca ccgtcagacg tcaggtagcc gactgcttct tcttcatgcg
     1981 agcgatcagg ccgctgaagc cttccttcgc gatgatcttg ttgaactgcg tcttgtaatt
     2041 ctcgacgagg ccaacgccgt cggttttgac gtcccacgct ttgagcttgt cgccctgctt
     2101 cttgaggatg tagtcgacgg agattttcag cgtgcggttc ttccgcttcg tgttgatcgt
     2161 cgtcgtgacg accgtattgc cgtctttgtc cgccgactcg ccggtgtagt cgaccgtgta
     2221 cgacaggttg gcgcgcaggc cgcgaatgta gttgtcctcg atcagcgcgc gcagcgtcgt
     2281 caagaactcc gtctgctggg ccgccgtcag cttgctccat tgatcggcca tcgcgcgctt
     2341 gccgagctcg tcgatgtcga ggaagtcgcg gacgctcgtc gtgaccttgc cggcgagctc
     2401 cttctcttcc ttgctcccgg cggccggctt ctgctttagc agcgccgaaa tcttgtcgtt
     2461 cgcggacttc actgcggtgg tgccggcacc agtcttggca tcggccgcaa acgccggggt
     2521 gccagcgaac aggacaagaa gaaggatgag tagacgcttc atgggcttac ctcggacgtg
     2581 ctgggccggc gtggaattca ccggtggcgc gtccgagacg taccacagcg acgttccact
     2641 ggaagaccga ctgggcccag cgagctttca tttgaaacca ggcgatatac gcatccgcga
     2701 gatccttggc ttcagcggtt ccgaccgcct gattttgcag gaccgaggcc aaccacgtgc
     2761 gtgccgcctt ttcgccctcg tttgccgcat cgaccttgcc cttcgcggac cgggcctccg
     2821 cgatcgccgt ttccgcatcg taggtcgcac cgatctgagc cagcgctgcc tgcgccgtcg
     2881 ccttgcgtgc ctcggcgcgc aaccgctcgg tcctcgctct cacagtccac ggctcgacgg
     2941 accactgcag cgcgagcaag agcgacagtc cctggcggtt gtacggatca ttcgcgtacg
     3001 cgctcggcgg atcgtccgcg ccctgcgctc tcgagatcac cgcactgccg acgagcgata
     3061 gatccgggaa gtagtacgcg gcctgaaagc tcgccagctc gttcgcggcg actgcaccgg
     3121 acttcgcagc gagcagctgc ggacgcttcg tcgcaccgtt cgccaaatcg gctgcctcgg
     3181 gtaccggctt ctcgacagcg agcagctcgt cttcatcgat gtctgcatcg ggcgtcgcgg
     3241 tgatcgcacg caatccggca agcgcttgcg cctcggcctg cgcggcctct gcacgctgca
     3301 ctttcgcttc ggcgagcagc acggcaacgc gctggcgatc ttcgatcgtc acgtccttgc
     3361 gatcttcgaa gcccttttga gcaccttcga tctgctcgat gccgtcctcg agcatcgcgc
     3421 cgagctcgcg cgccagcttg aggccccaat acgcgcgcgc cgcgtcgact gctgcatcac
     3481 ccgcggcctc gtcggcgagc gacttctgcg cggacacacc tgcgcgcgcc gcggcgagac
     3541 catgcgacgc cttgccgaac gtgtagagca cttgcgtaaa ctccagtgac gcgccgccga
     3601 agaagccgga gaacttgaac gcgaagttct gcggtgacgt gcgcgtgcag tccgcgttgt
     3661 cacactggat ctcggggctg accgttccgt agatcgtcgc ctttacgcgc ggcaatacgg
     3721 cggcgtacgc ctcgtgaatg cgcgactcgg cgacatcctt gtcgctgatc gccatctgca
     3781 ctttcggatt ggcggccgcc ttcgcgatca cctcatcgag cgtgagcttc ttcggttccg
     3841 ccgacgcgat cgtgggaacg agcaggagcg cgataacgag caagcgcatg ccgcggttgt
     3901 cccacgggga cgagcgcggg ccaaggtgcg ttacgccgcg cgatcgcgtg caggggcccc
     3961 gcagtgcggg cagctgacca gctcggcggg gtgcggcccg gcgcagtggt cgcagaacgt
     4021 cttgcccacg agcgcaccct ggatcgggat caaatcgggc gccggatacc gagcctccag
     4081 ctccatcgac gacatccgat catcgtcctc ggacggcatc cagatcacgt cgacagcgac
     4141 gagctggtcg ccgaggaacg ccgcgctctc gatcgcgttt cgcaggtcct cgccggtgcc
     4201 gatgcgctcg acggtgaaca gctcgctgcg cgccgcgatg acgatcgtca cgaggatgag
     4261 cccttcgcct tcgtcgctgc gccgcgagat gtcgcctgcg tgttggctcg atttcttgcc
     4321 gtcgacgttc gtgatcgtct cgcgcgagaa gcgcgtgcgc gcatcgtcta cgtggcggtc
     4381 atagatcgcc ttgatattcg agagcggccc cattcgttcg ttgcacgcac cgccgtagac
     4441 ccacgcatcg cgcgagttgc gaagcagcag cgagaccttg ccgagcatct cggcgcgccc
     4501 tgtcttcgtc gacgtattcg acgtctgcgc gatccacttc agcgcattct ggatg
//
LOCUS       DATLBZ010000483         7450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9560082, whole genome shotgun sequence.
ACCESSION   DATLBZ010000483 DATLBZ010000000
VERSION     DATLBZ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9560082"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VMZ53_24435"
     CDS             <1..445
                     /locus_tag="VMZ53_24435"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUS31682.1"
                     /translation="VFGVSAESAEAYAKWLSEKDGLKWRLPTEHEWEKAGRGTDGRIY
                     PWGDHFEATFCKMRDSRPGAPLAEAVGTYEWDISPYGVRDLAGSIADWCIPDERRTAK
                     REPREVVSRGGAWCDWAIDCRLASRRRYLATEHSARVGVRLARDP"
     gene            461..826
                     /locus_tag="VMZ53_24440"
     CDS             461..826
                     /locus_tag="VMZ53_24440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31683.1"
                     /translation="MENLSSGDLVIEVNVSPDAVYSCTWKGKSNERNPPEILKPWFEK
                     LLAAVTEKKGSIEMHFEKIEHFNSSTITALIKLIQVCRKSNVKLVMVYDQSLKWQRLS
                     FDALRVFEKNDDLFHLRFA"
     gene            823..1335
                     /locus_tag="VMZ53_24445"
     CDS             823..1335
                     /locus_tag="VMZ53_24445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31684.1"
                     /translation="MSVNADDFGFSIDLTVRNEWRNVDLLRTSVQNCFIAVFADLDGC
                     HAIAMVTGELLENALKYGNWASSERAMFRLRVVGNQGNIEVSVQNPLKPDDMNAQQLM
                     TALEWIASFPKPGEAYRARMMQIAQDESGDASPSRLGLVRIAFEGNCRLTAKIEKGAV
                     TVSALLLRDS"
     gene            complement(1322..3187)
                     /locus_tag="VMZ53_24450"
     CDS             complement(1322..3187)
                     /locus_tag="VMZ53_24450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31685.1"
                     /translation="MRRLAFVLLAVAACTDETPPTVAFDLDHPHTADTFWDMPFPSDL
                     RLTADGRPDLEGFPNKRNLPVVNDLLAGARERKGFPVMPIAYFRFTAEPPAHDLGPTD
                     DALIIDIDSTSPEHGTTFPVVLQSLPADAYSNGLVAVAPVPGIVLRGNTRYAVVIKKT
                     FAPGFSPPAAMHDPRVASLYAPLWAALNGSDDVLVATVFTTGDEVAILRERSEAIRAA
                     HTATIANLTRDPRTFDGFCSFTADVTFPQFQKGIAPYSTDGRFELDAQGIPIAQGAAT
                     VPLRITIPNGPMPSGGWPLWQYFHGSGGASFDLVDEGPSATADSDPLAGEGPGAIVAR
                     RGIAAASSSLPVDNERLPSAGAYDYLNINNLSAFPYTFQQGVYEQRLLLDALVELDVP
                     TCDGAAGHFDSAKLLAGGHSMGGMYTNMIAAIEPRYGAVTPFGAGGFWNMMILDTAIV
                     PGARGLLGSVLGVDGDTLTFMHPALGLIELGWEIADPINSMSRIAHRPLPGHTARHVY
                     QPIGLDDKYFPMPIFDAAALAYGNQQAGDMVWTTTQDSLRADGLDGLLSYPVHGNQGT
                     TAVVVQYRDGGIVDAHQIYRQLDEVKYQYGCFLQSYLRDGVPTVPAPAALSASCP"
     gene            complement(3259..4038)
                     /locus_tag="VMZ53_24455"
     CDS             complement(3259..4038)
                     /locus_tag="VMZ53_24455"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HUS31686.1"
                     /translation="MCELLGMECNVPTDIVFSFAGLASRGGKAGPHGDGWGLALYDGK
                     LARTFLEPHPAASSPLAKFVREHPIKTLLAIAHVRRKTRGGLALANTHPFVRELWGRH
                     FVFAHNGTVKKVKKLKLGRFKPIGETDSEHAFCALLGALEHEFKDYPRSQSDYAAAVA
                     AYAGKMGKDGTFNMLLGDGKQLFARCSTKLHYIVRKSPFNRATLVDEDLSVDFAEVTT
                     PNDRVAVVATMPLTRDEQWTAGTPNTMWWFRGGKLLKTLAS"
     gene            complement(4055..4996)
                     /locus_tag="VMZ53_24460"
     CDS             complement(4055..4996)
                     /locus_tag="VMZ53_24460"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HUS31687.1"
                     /translation="MNARWLYWIFVAAVAVALVALGAPSWAALGAGLVIAVMTTAAPP
                     RQLKLLTNIALQLGVVALGAGMDLGVVMRVGGTGVLITASSIAIALVVGHLLGKALRV
                     QGHTPLLISIGTAICGGSAIAAAASVLEPEEHETSVALGVVFMLNAVGLVLFPLVGQA
                     LGLSQPVFGRWAALAIHDTSSVVGAGLAYGSVALAVATTTKLARALWIAPLTIGIAVA
                     RRKRGSLRGVRWPWFILGFIGVAALVTFVPAVAKFGPEIVMVGRRALVLALFLIGLSL
                     SRAALSRVGTRPLLLGVALWLIVAIGSLPLALLPHTS"
     gene            complement(5045..6445)
                     /locus_tag="VMZ53_24465"
     CDS             complement(5045..6445)
                     /locus_tag="VMZ53_24465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31688.1"
                     /translation="MTSNGAARATSRAPSLQHRLVIAVAALTTVAIAIVGVALYGAAK
                     RGAWQQFDEALRGRAEAICGVAEHDEDGYEMELPPLQNAVATAYLPDGNIATTVDRRF
                     PPTTVLARSEAYGISDVRFPDKTSGRAIQLRCMPRDETGGAKPITLIVAERTDSVAVA
                     LDAIKPWFVAIALIAVLATSGIALLLVRRALSPLPQLATAISRIDDTSLDQRLSVAGQ
                     PAELVSPIDKLNDLLGRLSSAFARERQFTADVSHELRTPIAGLRTLLEVTALSERSTA
                     EYKTALVDAQAIVVQLAALVDNLLLLARLDAGQVPLATTDVDLAELVADCWKPHAAPA
                     SARKLEFRCTIPASTRITVDREKLRSVLGNLLANAAEYTDEGGWIEVSSSPEALLSVT
                     DSGPPIPREQLAKLFDRMWRGDASRGDTGVHCGIGLALARSLCAAMSLELTAETRDDG
                     SVRFTVADPASRRIGR"
     gene            complement(6442..6783)
                     /locus_tag="VMZ53_24470"
     CDS             complement(6442..6783)
                     /locus_tag="VMZ53_24470"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS31689.1"
                     /translation="MRVLVRRRYGATEKAIRIADLEIDTQARRVNRGGAVIALSAREY
                     ALLEYLAHRVGHIVSRTELWDHAYDEAAEPGSNVLDVHISHLRRKIDDGHDTKLIHTK
                     RGQGYVLGEDA"
     gene            complement(6790..6990)
                     /locus_tag="VMZ53_24475"
                     /pseudo
     CDS             complement(6790..6990)
                     /locus_tag="VMZ53_24475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(7119..>7450)
                     /locus_tag="VMZ53_24480"
     CDS             complement(7119..>7450)
                     /locus_tag="VMZ53_24480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31690.1"
                     /translation="NARVTSESEGGTLIYEGARETKLEVEVSAQGQLMQTEVAVPVAA
                     LPDAVTKAFAGKGAIKEAEVVLRASGVAFEIEVGGTEYVVDAQGNVLSTEAGEDGDGE
                     GADEDRD"
BASE COUNT         1300 a         2350 c         2587 g         1213 t
ORIGIN      
        1 cgtgttcggt gttagcgcgg agtcggccga ggcgtatgcg aaatggctct cggagaagga
       61 cggcctgaag tggcgcctac cgacggagca cgagtgggag aaggcgggac gcggcaccga
      121 cggacgcatc tatccgtggg gcgatcactt cgaggcgacg ttctgcaaga tgcgcgatag
      181 ccgccccggc gctcccctcg cagaagccgt cggcacatac gagtgggaca tctcgccgta
      241 cggtgttcgc gacctcgccg gcagcattgc tgactggtgt attccagatg aacgtcgcac
      301 ggctaagcgt gaaccgcgcg aggtcgtgtc acgtggtggc gcgtggtgtg attgggcgat
      361 tgattgccgg ctggcgtcgc gccggcgtta tctcgcgacg gagcactctg cacgggtggg
      421 cgtgcggctt gcgcgagatc cgtaaaggac gaggagaatc atggagaacc tgagctctgg
      481 cgatctagtc atcgaggtga acgtctcacc ggacgcggtc tacagctgca cctggaaggg
      541 caagagtaac gagcgcaacc cgcccgagat tctgaagccc tggttcgaga agctgctggc
      601 tgcggttacc gagaagaagg gctccatcga gatgcacttc gagaagatcg agcacttcaa
      661 cagctcgacg atcacggccc tgatcaagct gatccaggtc tgccggaagt cgaacgtcaa
      721 actggtgatg gtctacgacc aaagcctcaa gtggcagcgc ctgtcgttcg atgcgctccg
      781 cgtgttcgag aagaacgacg atttgttcca cctgaggttc gcatgagcgt taacgcggat
      841 gattttgggt tctcgatcga cctcacggtt cgaaacgagt ggaggaacgt cgacctgctt
      901 cggacgtccg tgcagaactg tttcatcgcg gtgttcgcag atctcgatgg ctgtcacgcg
      961 atcgcgatgg tcaccggcga gctgctcgag aacgcgttga agtacggcaa ctgggcatcg
     1021 tcggagcgcg cgatgttccg gctgcgtgtc gtcggtaacc aaggaaacat cgaggtgagc
     1081 gtgcagaacc cgctcaagcc cgatgacatg aacgcgcagc agctcatgac ggcgctcgag
     1141 tggatcgcta gcttcccgaa gccgggtgag gcctatcgcg cgcgcatgat gcaaatcgcg
     1201 caagacgaga gcggtgatgc atcgccgagc cggctcggtc tcgttcgcat tgcgttcgaa
     1261 ggcaactgcc ggctgaccgc gaagatcgag aagggcgccg tgaccgtcag cgcgctgttg
     1321 ctcagggaca gctagcggac agagccgcgg gtgccggtac cgttggcaca ccgtcgcgca
     1381 ggtagctctg gaggaagcat ccgtactggt acttcacctc gtcgagctgc cgatagatct
     1441 ggtgggcatc gacgatgccg ccatcgcggt attgaacgac caccgctgtg gtgccctggt
     1501 tcccgtgcac gggataggac agcaggccat cgaggccgtc ggcacgcagc gaatcctgcg
     1561 tcgtcgtcca caccatgtcg ccggcttgct ggttgccgta cgcgagcgcg gcggcgtcga
     1621 agatcggcat cgggaagtat ttatcgtcga ggccgatcgg ctgatacacg tggcgtgcgg
     1681 tatgtccggg aagcggacga tgcgcgatgc gcgacatcga gttgatcgga tcggcgatct
     1741 cccagcccag ctcgatgagg cccaaggcgg ggtgcatgaa cgtgagcgtg tcgccgtcga
     1801 caccaaggac gctgccgagg aggccgcgcg cgccgggcac gatcgcggtg tcgaggatca
     1861 tcatgttcca gaagccgccc gcaccaaacg gagtgaccgc gccgtagcga ggctcgatcg
     1921 ctgcgatcat gttcgtgtac atcccgccca tcgagtggcc gccggccagg agcttcgcgg
     1981 agtcgaagtg ccccgctgcg ccgtcgcacg tcggaacgtc gagctcgacg agagcatcga
     2041 ggagaaggcg ttgctcgtag acaccttgct ggaacgtgta ggggaatgcc gagaggttgt
     2101 tgatgttgag gtagtcgtac gcgccggcgc tcggcaggcg ctcgttgtcg acgggaagcg
     2161 aggacgatgc ggcggcaatg ccgcgacgcg cgacgatcgc gccaggacct tcgccggcga
     2221 gcgggtcgct gtccgcggtg gcgctggggc cttcgtcgac gagatcgaac gacgcgccgc
     2281 ccgagccatg gaagtactgc cacagtggcc agccaccgga gggcatcggg ccgttcggga
     2341 tcgtgatgcg cagtggaacg gtcgccgcac cctgcgcgat cgggatgccc tgcgcgtcga
     2401 gctcgaagcg gccgtcggtc gagtagggcg caatgccctt ctggaactgc gggaacgtca
     2461 cgtccgcggt gaacgagcag aagccgtcga acgtgcgcgg gtcgcgcgtg aggttcgcga
     2521 tcgtcgccgt gtgcgctgcg cggatcgcct cggagcgctc gcggaggatg gccacctcgt
     2581 cacccgtcgt gaagacggtc gcaacgagca cgtcgtctga tccgttcaat gcagcccaca
     2641 gcggcgcgta gaggctggcg acgcgcgggt catgcatggc ggcgggaggc gaaaagcccg
     2701 gtgcgaaggt cttcttgatg acgacggcgt agcgcgtgtt gccgcgcagc acgatgccgg
     2761 ggacgggcgc gaccgcgacg agtccgttgg agtacgcgtc ggcgggcaag gactgcaaca
     2821 cgacggggaa cgtcgtgccg tgctcgggcg acgtcgaatc gatatcgatg atcagcgcat
     2881 cgtcggtcgg gccgaggtcg tgcgcaggtg gctcggcggt gaagcggaag tacgcgatcg
     2941 gcatcaccgg gaagcctttg cgttcccgcg cgccggcgag caggtcgttc acgaccggta
     3001 ggttgcgctt gttcgggaag ccttcgagat cggggcgacc gtcggcggtg aggcgcaggt
     3061 ccgacgggaa cggcatgtcc cagaacgtgt ctgctgtgtg cgggtgatcg agatcgaatg
     3121 cgaccgtcgg cggggtctcg tccgtgcagg cggcgacggc gagcagcaca aaagcgaggc
     3181 gacgcatgcc gtttcgtacc gcgtcacccc cggcgggacc gaggaataca atctgccacg
     3241 ctgccaggcg gcgtcaggct aggaggcgag cgtcttcagg agcttgccgc cgcggaacca
     3301 ccacatcgtg tttggcgtgc cggcggtcca ttgctcgtcg cgcgtgagcg gcatcgtggc
     3361 aacgacggcg acgcgatcgt tgggcgtcgt cacctcggcg aagtcgacgc tgagatcttc
     3421 atcgacgagc gtcgcgcggt tgaacggaga cttccgcacg atgtagtgga gcttcgtcga
     3481 gcagcgcgcg aacagctgct tgccatctcc gagcagcatg ttgaacgtgc cgtccttgcc
     3541 catcttgccg gcgtacgccg cgaccgccgc cgcgtagtcc gattgcgaac gcggatagtc
     3601 cttgaactcg tgctccagcg cgccgagtag tgcgcagaac gcgtgctcgc tgtcggtctc
     3661 gccgatcggc ttgaagcgac cgagcttgag cttcttgacc ttcttcaccg tgccgttgtg
     3721 cgcgaacacg aaatggcgcc cccacagctc gcggacgaat ggatgcgtgt tcgcgagggc
     3781 gaggccgccg cgagtcttgc gtcggacgtg tgcgatcgcg agcagcgtct tgatcggatg
     3841 ttcgcggaca aacttcgcca gtggcgagct cgctgccggg tgcggctcga ggaacgtgcg
     3901 cgccagcttg ccgtcgtaga gcgcgaggcc ccagccgtcg ccgtgtggac ccgccttacc
     3961 gccacggctt gcgagcccgg cgaacgagaa gacgatgtcg gtcgggacat tgcactccat
     4021 cccgagcagc tcgcacatgc gcgacgagag tacctcagct ggtgtgcgga agcagcgcga
     4081 gtggaagcga tccgatcgcg acgatgagcc acaacgcgac gcccagcagc agcggccgcg
     4141 tgccaactcg ggaaagtgca gcgcgcgaga gcgacaggcc gatcaagaac agcgcgagga
     4201 cgagcgcacg ccgccccacc atgacgattt ccggtccgaa tttcgcgacg gcagggacaa
     4261 acgtgacgag cgccgcaact ccgataaagc cgaggatgaa ccacggccag cgaacgccgc
     4321 gaaggctgcc gcgtttgcgt cgcgcgacgg cgatgccgat cgtgagcggt gcgatccaca
     4381 gggcacgcgc gagcttggtg gtcgtggcga cggccagcgc gacggagccg tacgcaaggc
     4441 ccgcgccgac gaccgagctc gtgtcgtgaa tcgcgagagc tgcccaccgg ccgaacacag
     4501 gttgcgacag tccgagcgcc tgaccgacga gcggaaacag cacgagtccg acggcgttca
     4561 gcatgaacac gacgccgagc gcgacggacg tctcgtgctc ttccggctcg agcacgctcg
     4621 ccgccgcggc aatcgcgctg ccgccgcaga tcgcagtgcc gatggagatc agcagcggcg
     4681 tgtgaccttg gacgcggagc gccttgccaa gcagatgccc gacgacgagc gcgatcgcga
     4741 tgctcgatgc agtgatgagc acgccggtcc cgccgacgcg catgacgacg ccgaggtcca
     4801 tgccggcgcc cagcgcaacg acgccgagct gcagcgcgat gttggtcagc aacttgagct
     4861 gacgaggcgg cgcggcagtc gtcatgaccg cgatcacgag acccgctcca agtgctgccc
     4921 agctcggcgc accgagcgcg accaacgcga ccgcaacggc ggcaacgaag atccaataca
     4981 gccagcgcgc gttcacgcgg tgcccttatc tcacgcgcat ccgacaccga cgaggacttc
     5041 cgttttagcg tccgattcgc cgcgacgccg gatccgcaac ggtgaagcgc acgctgccgt
     5101 cgtcgcgagt ctcggcggtg agctcgagtg acatcgcggc gcacaagctt ctcgcaagcg
     5161 cgaggccgat gccgcagtgc acgccggtgt ctccgcggga tgcgtcgccg cgccacatgc
     5221 gatcgaagag cttcgcgagc tgctcgcgcg gaatgggtgg cccgctgtcg gtaacgctga
     5281 gcagcgcctc ggggctcgac gagacttcga tccagccgcc ctcgtcggta tactcggcgg
     5341 cattcgcgag caggtttccg agcacgctgc gcagcttctc gcgatcgacg gtgatgcgcg
     5401 tcgaagcggg aatcgtgcag cgaaactcta gcttgcgggc cgaggcgggc gcagcgtgcg
     5461 gcttccagca gtccgcgacg agctcggcga ggtcgacatc cgtggtcgcg agcggaacct
     5521 ggcccgcgtc gaggcgtgcg agcaggagca ggttgtcgac gagcgcggcg agctggacca
     5581 cgatcgcttg cgcgtcgacg agcgcggtct tgtactcggc ggtcgagcgc tcggagagcg
     5641 cggtgacttc gaggagcgtg cggaggcccg cgatcggcgt gcgcagctcg tggctgacgt
     5701 cggcggtgaa ctggcgctct cgcgcgaacg cagacgacag ccggccgagg agatcgttca
     5761 gcttgtcgat cggagagacg agctccgcgg gctgcccggc cacggagagg cgttgatcga
     5821 gcgaggtgtc gtcgatacgg ctgatcgccg tcgcgagctg gggcagggga gagagtgcgc
     5881 ggcggacgag caggagcgcg atgccactcg tcgcgagaac cgcgatgagc gcgatagcga
     5941 cgaaccacgg cttgatcgca tcgagggcga ccgcaaccga gtcagtgcgt tccgcgacga
     6001 tcaaggtgat gggcttcgcg ccgccagtct cgtcgcgtgg catgcatcgc aactgaatcg
     6061 cgcggccact cgtcttgtcc ggaaaccgga cgtcagagat accgtacgcc tccgagcggg
     6121 ccagcacggt ggtcggtggg aatcggcggt cgaccgtcgt cgcgatgttg ccgtcgggca
     6181 gatacgccgt cgctaccgcg ttctggagcg gcggcagctc catctcgtac ccgtcctcgt
     6241 cgtgctcggc gacgccgcag atcgcctccg cgcggccacg tagtgcttcg tcgaactgct
     6301 gccaggcgcc acgcttcgcc gctccgtaga gcgcgacacc gacgatcgcg atcgccaccg
     6361 tggtgagcgc agcgactgcg atgacgagcc ggtgttgcag cgagggcgct cgactcgtcg
     6421 cacgcgcagc gccgttcgac gtcacgcatc ctcgccgagg acgtatccct ggccgcgctt
     6481 cgtgtggatg agcttcgtat cgtggccgtc gtcgatcttg cggcgcaggt ggctgatgtg
     6541 gacgtcgagg acgttgctgc ccggttccgc ggcctcgtcg tacgcgtgat cccacagctc
     6601 ggtgcgcgac acgatgtggc cgacgcgatg cgcgagatat tcgaggagcg catactcgcg
     6661 agccgagagc gcgatgaccg cgccgccgcg attcacgcga cgcgcttgcg tatcgatctc
     6721 gaggtcagcg atgcgaatcg ccttctccgt ggcgccgtag cgtcgacgta cgagcacgcg
     6781 cacgcgagcg agaagctcgg caaatgcgaa cggcttgatc atgtagtcgt ccgcgcctgc
     6841 gtcgagccca gcgacccgat cgggcacggc gtccttcgcg gtgagcacga ggatgggcgt
     6901 cgtgttcccg gccttgcgca gcttcgcgag gatcgtcatg ccgtcgatgc ccggcagcat
     6961 gaggtccaat acgatcgcgt cgtacggatg gttctccgcg taccagagcc cctaggtacc
     7021 gttgcccgtc gcgtcgacgg cgtagcccga atcgcgcaag cccttggcaa gcgactcgcg
     7081 caacggtccg taatcctcga cgacgagcag ccgcatgctc agtcgcgatc ctcgtcggcg
     7141 ccttcgccgt cgccgtcctc gcccgcctcg gtcgagagca cgttgccctg cgcatcgacg
     7201 acgtactccg tgccgccgac ctcgatctcg aatgcgacgc ccgacgcgcg caacacgact
     7261 tcggcctctt tgatcgcgcc cttgcccgcg aacgccttcg tcacggcatc aggcaacgcc
     7321 gcgaccggca ccgccacttc cgtctgcatc agctgcccct gcgcgctgac ctcgacctcc
     7381 agcttcgtct cccgcgcgcc ttcataaatc agcgttccgc cctcgctctc actcgtcacc
     7441 cgcgcgttcg
//
LOCUS       DATLBZ010000484        26726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1364818, whole genome shotgun sequence.
ACCESSION   DATLBZ010000484 DATLBZ010000000
VERSION     DATLBZ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 26726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26726
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364818"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2073)
                     /locus_tag="VMZ53_24485"
     CDS             complement(<1..2073)
                     /locus_tag="VMZ53_24485"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HUS31691.1"
                     /translation="MWSDLRNALDAGATIAYGSTPGWAAWLAAQLAAREALVVVVAAD
                     DAAARELESDIRFFLGGQRDATELDAVASLPGIDVSPYAELSPDRACIVERVATLYRL
                     TQPALRPRMVVTSAEALARRTVPAGELATRGVTIKKGETIDRDQVAALLVAGGWARTP
                     VVDEPGTFAVRGGVIDVYAPLSPHPVRIELFGDEVESLRWFDAESQRTLRQVEHVHLH
                     PVRETINTGTREVRTRLREYADEIAFPSKATRRAIEQLEAGDAFVGIEGLTPAFHDEL
                     VAPETYVPADARWLVVDPDACRRVISDLWKDEEARYEQRDQKQLAFVPARHFVEELGA
                     FSAPLRIELSSLEVHGDSDATKLRVEVDNLRVLRQQLDHARTFGDTEHVAPLLDALKK
                     WKDLRVAIACDSQSRVDRLHGVLEARGISVRIATAGEAPLATSGVTLIPGAPTHSFAS
                     PRDGVVLVTATDIFGERVHHPSNKQKRAKDALLGGVSDWSQLQAGDYLVHQKHGVGRY
                     HGLKKIAVGTVSILQTTAVGAPTPKATEIDALHLEYDGGTLYLPVYRLGEVQRYVGAE
                     GHAPKIDKLGGVTWEATQSKVKRHVRVLAEELLQIYAQRKSLPGHTFPPPDDSYRELE
                     ATFPFDETPDQLDAINAVLGDMETDRAMDRLVCGDVGYGKTEVALRAVFKAVQGGKQA
                     CMLAPTTVL"
     gene            complement(2141..3154)
                     /locus_tag="VMZ53_24490"
     CDS             complement(2141..3154)
                     /locus_tag="VMZ53_24490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31692.1"
                     /translation="MSQDDESPEIEMTADEPTPVPAEVVQKQRRRRAANPTADEPIHT
                     GEIAPRSMIIRSSPSMEIPKIEIAKIEISDEKTELAPPPIMEPEPVAAPPRRARAVTA
                     GVRRRSMAPIFIGVGVAIAGVVVAVVLVGKSKPAATTTTPANSASDQHRLPLQAAAQF
                     IGTTFDADAKAALVRAEAMATSSMLRAGILTDAQTLQDMAKDKDVVFKLEAGDTVEVI
                     QVRDGTRTPMLRLPGDAAALDAPPVGKTKIVLRNGAPIVIATAEIAEQQGTSGQVVLG
                     SPVDLEAIKKRVAGHAVEASLVGLGAPIVLAKTGTPGGETIKLPIENEAKLPLELDVV
                     LAK"
     gene            complement(3222..3875)
                     /locus_tag="VMZ53_24495"
     CDS             complement(3222..3875)
                     /locus_tag="VMZ53_24495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31693.1"
                     /translation="MKTLAITMVLAMAGTAYADQCAWIDAKAATKAKKIVESHPKVIA
                     FCEPCGEKAPGIPEAATSVEIGTPDGGFKELQINGTGVDLAYTYVQMSPGLYRNLAML
                     AGCEVTDVSPSLKIADETPNGVLITADPAPPPPVVEPQLAPPPPIVLTLPTAAPPPPP
                     QIVVLETRPAFPWAMIALAGMTGFGATLFAVLAIATLRRRRAMRPRATELRADHSDK"
     gene            complement(3872..4576)
                     /gene="xrtK"
                     /locus_tag="VMZ53_24500"
     CDS             complement(3872..4576)
                     /gene="xrtK"
                     /locus_tag="VMZ53_24500"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase K"
                     /protein_id="HUS31694.1"
                     /translation="MTFVREHRWQIAAVVITLAAVIAGKQFYRSASAEDLRFLLAPTA
                     HLVSWLTGGNFVYEFGAGYADYKIGFIIAPPCAGMNFALAAFVTLVFGSLSAITGPRA
                     LAVRLAFAAVATYLATIVVNTIRISIAVAMHRGHLRVGDLDRAEAHRLEGIVVYLGGL
                     LLLFALARAIEARKRRATFAIPIAAYLVITLGLPLANGAAARGEFAHHALLVVTACTV
                     VVALLLGVAHYKRRTI"
     gene            complement(4573..6543)
                     /locus_tag="VMZ53_24505"
     CDS             complement(4573..6543)
                     /locus_tag="VMZ53_24505"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HUS31695.1"
                     /translation="MRILALVLALLVVPRVVHADNTDKSLSPFFFVEGAAPGVEALPL
                     ESTKADVHISGVIADVTVKQMYRNDGEKTISAKYVFPASTRAAVYGMKMTIGDRVIVA
                     KIKERQEARKEYEEAKAAGKTASLLEEDRPNVFSMNVANILPKDRIEVELKYTELLVP
                     TDGVYELDYPTVVGPRYASDNLDAKNPTNQFVASPYTKSGAQPSYAFDINVSVAAGMK
                     IQSIDSPSHTIKTSMGSAGTSANIALVNGSATGNKDFVLRYRLAGDDITPGLLLFPGQ
                     KENFFLMMVQPPHRPALEMIPAREYVFIIDVSGSMNGFPLDTTKTLMRDLLGKLRPTD
                     HFNVLLFSGGSSLYSEKSVPAEKDKIADAISFIDREQGGGGTELLPAMQRAMKLPRLS
                     KDLSRSFVVVTDGYIGEEAEMFEHIRAHLGEANVFSFGIGSSVNRHLVDGVAQAGQGE
                     PFVILDPKQAPDAAKKFREYVESPVLTNVEVKFDGFNAYDLSSKSIPDVFANRPVVVF
                     GKYKGTASGKITLTGVSGRGRFVATVEPAKFSADASNQALSYLWARNKVSELSDFTFG
                     DENKEAVTKLGLDYNLLTKYTSFIAVQQIVRTTAGSTDVTQPLPMPEGVSDLAVAESG
                     NEVGAEPGLIILLVLVGAVLILTQRRAARGVK"
     gene            6632..7720
                     /locus_tag="VMZ53_24510"
     CDS             6632..7720
                     /locus_tag="VMZ53_24510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31696.1"
                     /translation="MTRFAIVAALTVAGAAHADTPHDVARVRGLKVKKPIAQEIVDRA
                     DLQSHLLSKKTAAELEAEAIARTRWGLAAPVTNDDQVTGTYDPATKKLVLVTPVEEFV
                     LVHEVEHALQDMHFDLSKVEGGTGDEAIARHALVEGDAIVTTLEVLLDRKGVDPPWDN
                     PQVVAELIRAFDLPVDESYKAGFAFVVGLRAQKPWSAVDAAFKRPPRSTEQVLHVEKY
                     LADEQPVAVETLVVADARIIHETTWGELGVQQFLRAHGIDPQTAKLAAAGWAGDRVTT
                     LEKNGRVIGLARLVFDTPADAMEAFDAIERAIDDSQLAARAENSEARTRWLALDGTVS
                     TLERRGEEITIELAIPLAQIGSALPSSR"
     gene            7717..8079
                     /locus_tag="VMZ53_24515"
     CDS             7717..8079
                     /locus_tag="VMZ53_24515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31697.1"
                     /translation="MIRVTALIVAMSVAGCGLTMTQGPNPSQPADQRPVCTETFAASR
                     RDGVGAMVGLVAILFGTIAVKGADNTTVGVPLLVGGGVVIVGAYISGGVGYMRVKKCK
                     KAIEAFERRTVSVPPATP"
     gene            complement(8081..8566)
                     /locus_tag="VMZ53_24520"
     CDS             complement(8081..8566)
                     /locus_tag="VMZ53_24520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31698.1"
                     /translation="MNGEWFWLGVVLLLAAALILPRLFPEPVSEPRAPEPTPLAWNPE
                     RRRWTQQIIFAGQVIEVALDGDPSGPSAEAMGTWLDLRGQLDETWQSVIKFTLRETAR
                     NGVQLYEPDEFGIKRVDLCPEDPQHGGDVIFAFEVASELGTVFTVPLRHGKPLSLQRS
                     N"
     gene            complement(8563..9726)
                     /locus_tag="VMZ53_24525"
     CDS             complement(8563..9726)
                     /locus_tag="VMZ53_24525"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HUS31699.1"
                     /translation="MPRLVDVLLERRIIVCVGSGGVGKTTTAATLALAAARRGKRTLV
                     LTIDPAKRLANSLGLSALGHQVQEVDRALVRTGAPAQTGELHAMMLDQKMAFDEVVHR
                     HAKDPAAVKRILANPVYAQISGSLAGAQEYAAMAKLHDFDESGQWDTIIVDTPPTAHA
                     LDFLDAPRKLSEAIDSPAIEWFRKLQGGSGSGWSFVGKTGAFVLKRLAKFVGSQFIDD
                     LGVFFTEFNDILGGFRTRAEETFSLLRKPRVGFVLVASPEPMAVREALFFHERLVEAS
                     MPFVGFVVNKVHPALPIRADEPTLAAALGAHPGVAALGISGTTRTMTAHALFAAHGEL
                     ETLAEADRAAIAQLRSAGGANTLLIEVPLMRDDVHDVSRLVGLERYLLGAEAR"
     gene            9759..10271
                     /locus_tag="VMZ53_24530"
     CDS             9759..10271
                     /locus_tag="VMZ53_24530"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HUS31700.1"
                     /translation="MKRELIVTADGRDRTVTVEGPLPDGRFRVTIDGTAHDVDGKAIR
                     PGTWSLVIAGRNYVVDLDKRRTGIAASVGASEAMLQVEDALHRRLAAAASPRDKARGE
                     TLRAPIAGKVVKLLVAVGDDVVPGAPVIVLEAMKMENEIVSERGGKVTAINKQAGQAV
                     DTGDVLVELS"
     gene            complement(10277..11884)
                     /locus_tag="VMZ53_24535"
     CDS             complement(10277..11884)
                     /locus_tag="VMZ53_24535"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUS31701.1"
                     /translation="MGADGKSEAMARQLDAAQAITHVGSWEWDLASGVVSWSAELYRI
                     YGLVPGERTITLEVFLGALHPDDRDRIQAEVTAAMQRGGRFAWLERVVRPSGEVRVLD
                     TIGEVIKSDAGAPVTLIGTCRDITEEAELAKARERVQRVQAGERQALELLAANAPLEE
                     VLAVIVALIEELSPRTIASVLLLDERGAHLKHGAAPNLPDAYNRVIDGAAIGPKAGSC
                     GTAAYRREAVLVRDIATDPLWLLYRHLVEPYGLLACSSFPILASDGRVIGTFAVYYKE
                     QRIPDAASRELIERAAHVAGIAIERRQLDDQLRKLPGRIEEAREEERTNIAREIHDEL
                     GQALTALKLDIALVCRRLRNGNGHGDELPKKLEEMASTTDEIISSVRRISAELRPGIL
                     DDLGLRAALEWQAEEFTRRTGTPCHVKADVADLHLERGLATAVFRIFQEALTNVTRHA
                     QATFVECHLWLERGMLKLDIADDGVGVPMIAPRSSSLGLLGMRERARRCGGECTIRRR
                     EPRGTLVALSVPLKFPSAREEDHELGA"
     gene            11916..12542
                     /locus_tag="VMZ53_24540"
     CDS             11916..12542
                     /locus_tag="VMZ53_24540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS31702.1"
                     /translation="MHRILLVDDHVVVRRGVRAILEDHFPGIHVAEANNGDEALAALA
                     QPFDAVVLDLSMPGRSGFDLLSEIKHRYPKLPVLIMSLHDEEQFAVRALRAGAAGYLT
                     KAAASEQLVAAISKITRGSKYISDTLAERLATDVGGDHIASRHERLSDREFDVMRGIA
                     SGSSVSEIAAQMHLSVKTVSTYRTRLLEKMGMSTNAELTRYAIENGLV"
     gene            12637..13683
                     /locus_tag="VMZ53_24545"
     CDS             12637..13683
                     /locus_tag="VMZ53_24545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HUS31703.1"
                     /translation="MTNTGCMKVLIVDGSKQKRSNLVEALGELTNVVVQGAVSHVRTA
                     IRVVADATPDVIVTGVVLPDGEGTHLIERVRRMTAPPSIVVVADPGSDEQRSRYLAAG
                     ADRYVDSDDVGEVQAAVAGLRAREGVSPTESQRLLGRMTTGVVHDLNNYLAVLDGSLE
                     LMHRNPQDLAELWPPVRTTMDAIQDLVGTLLAYARGGTPPVEELDVGEVVRHAIQIGR
                     RMVPTNVVLTVEIAGGLRRLHGIRAELQQMILNLVVNACDAMPLGGELNVAVKGATAQ
                     AVLVEVSDTGSGKPFMPLVAGRTQSTKKGRFGIGLGMGIVHAVVDRHKGAIRVLERAG
                     GGTLVAVMLPTRAE"
     gene            13698..13916
                     /locus_tag="VMZ53_24550"
     CDS             13698..13916
                     /locus_tag="VMZ53_24550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31704.1"
                     /translation="MRWLVVVLIVATACRAERTNHCPSNVTSNCISGEVCSMDRQRGC
                     QVCQCRPWDGTPRSKDPDDEGTPPIPVH"
     gene            complement(13921..14625)
                     /locus_tag="VMZ53_24555"
     CDS             complement(13921..14625)
                     /locus_tag="VMZ53_24555"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUS31705.1"
                     /translation="MTRFAALLLVLAACSGGEDITYHWDRDRVLCSVPIDDLSHEVAW
                     DKVDEQLDNAAAHGSVAILHAHKPGITVSLASIERALTSAAERGLPTITFRELDGPPR
                     GALAFAFDDNSPELWLTAQDLLAAHDARITLFVSRWTTMTDAQRADVMTLASLGHDLQ
                     PHSVAHVDAVEYVAANGVDAYVADEVLPSFTPLEQLGYPPTTYAYPFGSHTAEIDLAV
                     LAHISRVRAAGECDEP"
     gene            complement(14622..16049)
                     /locus_tag="VMZ53_24560"
     CDS             complement(14622..16049)
                     /locus_tag="VMZ53_24560"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUS31706.1"
                     /translation="MSAHPEVVRVFATSQDPDFDGPWQARTPSNSTGSAVVIGPGLLL
                     TGAHVVANATFMQIQKVSAPDKAIARVKAVSHDCDLALLEVVEPADFLADIEPAEVGD
                     MPRLRDEVAVVGYPVGGEEISITEGVVSRIEVQRYSHSQRHLLAVTVDAAINAGNSGG
                     PVFGDGKVVGIAFQKLTGVDNIGEMVPPPIIRSFLDGVEKGKRPEIPALGITTQNLEN
                     PLLRKQLGLGDGERGVVVLHVDYGGSAYGILQERDVITSIDGLAIANNGTVQFMNRYR
                     TRYDVVLGYQYIGDKIKLDIKRAGKLKTVEVELKAWQPLVPRSRYDQPPQYFVYGGLV
                     FQTLTRDYLTTWDKWWNKAPKEFLNYYYLGYRTAEQHEVVILTQILADEINVGYAHLY
                     NEAVATLNGKAPLDMVDFVQQLSAARGVVEINTTSGGVIMLDADEVRKATPRILTRYH
                     IPRDRTPGLPGAPAAATPPIRAAMP"
     gene            16120..17139
                     /locus_tag="VMZ53_24565"
     CDS             16120..17139
                     /locus_tag="VMZ53_24565"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUS31707.1"
                     /translation="MHALQSITPERLAALVPVITLGEARKLVAQVHRGEPIAPTSAIR
                     RTAAEAVRAIGEVRTLEVIAETASAIDPFVKYLLGSGDARFEAVRIPLEHPDRFSVCV
                     SSQVGCALACAFCATGRLGLTRNLEAWEIIEQVRVVRARLSRGRVHGVVFQGMGEPLA
                     NLDEVLTAIDVMCEPCALGIDARAITVCTAGLPVGVRRLAREAPKVRLGLSIHTVLPE
                     RRKSLMPIARAHAIDELLDAAVEHARITGLSPMWAVTLLDGVNDSDDDAHALAERARW
                     FEAQTGRLPRISVIEYNAIGDGDVFTRSPRVVEYRKALGLPSHRRYSGGSDVAAACGQ
                     LALRT"
     gene            complement(17136..17723)
                     /locus_tag="VMZ53_24570"
     CDS             complement(17136..17723)
                     /locus_tag="VMZ53_24570"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HUS31708.1"
                     /translation="MAHPVHDIEVPSDIRTWVPSVIEIPRGSHLKYEVDKPTGLLRLD
                     RVLYSAVHYPANYGFIPRTHADDGDPLDILVLMQEPVAPLTIVRARALGGLRMVDDKG
                     GDDKIVAVCVDDPAYEQYQSLEQLPPHVMKELDRFFRDYKTLEEKMVEVDRFYGHDKA
                     FEIIQTSRDAYDRDSASLRDAARSSEAGPFDRGER"
     gene            complement(17735..18346)
                     /locus_tag="VMZ53_24575"
     CDS             complement(17735..18346)
                     /locus_tag="VMZ53_24575"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUS31709.1"
                     /translation="MLLYHDPRAPNPRRVRIFLAEKNVAYDTIEVLIADRAHQTDDFR
                     RKNPLSLLPVLELQDGRILRESMAICRFVEEEHPEPNLFGANSWERAQVEMWNRHAEL
                     ELLVPISQVFRNTHQFWVGRIKQAPDFAEIMREQLALRFDWLEGELSRRQYMAGDRFT
                     VADITALCALDFGKVSNVRINAETHPNFAAWHKRVSERPSMKA"
     gene            18407..19315
                     /locus_tag="VMZ53_24580"
     CDS             18407..19315
                     /locus_tag="VMZ53_24580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31710.1"
                     /translation="MRIAGLLLLVCLAGGCKNKGQDADAAPDPAALKAQQELIARRDK
                     LLDARQKLQQDRDKIDAEIKDIQAKGGDASEQLKKREELDTQIETSTSDLIGMVNSKL
                     DSLSKDKSAQVAARESDLASREKTIAEREARIAERERLLAQRDAESAARWKDTCSTGA
                     APVIIQQTAPKGGNWTNKDVTALLQKARNAMNKKGLINSDLPGPAASFESEATKAMSD
                     NDSNRAYIAAANLVGTIDTIVVNRQFIQQKTARIQSQAKAIKLDEATNKDLQDIIGDV
                     MNSYNNGDFAAANKRLNQAAAKIAKQ"
     gene            complement(19320..20201)
                     /locus_tag="VMZ53_24585"
     CDS             complement(19320..20201)
                     /locus_tag="VMZ53_24585"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS31711.1"
                     /translation="MRLTPLVIVLVAATAARAYADRVVAIAPLSTLGAEDKSAATKKL
                     LAQIEQAIAALPGTKIVPAAQVSAAIDKAKKPQLKQCEDDTQCIAEVGKLVGAEQVVT
                     GEVGGLGDSRVIYLKTTDVSKGQELRSTTLAIGSKDAADTPAGAAVRLLDPDKYRGNV
                     RFNFDVTGASVAVNGTKVNLSAAKDIALPVGTHAVTVTHPQYHNFVKFVDVKYGTVTD
                     VQVGMKQYPIVEGGIHAKPGASDTIIYTDPPVWKRWYVAGPAIFVLAVGVGIVAGVVA
                     HDFPQFDNCRKVGGENC"
     gene            complement(20192..21079)
                     /locus_tag="VMZ53_24590"
     CDS             complement(20192..21079)
                     /locus_tag="VMZ53_24590"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS31712.1"
                     /translation="MARVASFVLAVIALASAAAHAQEAPSAEDLLDKKIAVWRFDALG
                     IENEIVQRLETLFRMELDRLDKVPQPSRRDIERTVSAAEQNCTGEEKCLVSIGKKLGV
                     EFVVTGTVGSLGDNYVLNIKVVEVATGKSQKIQSDPLRGSPDELIEGVRVAAYRLLAP
                     QQLHGALQIQTDLVGAEVALDGTKVGATPLPNGGVLGKLKLGKHKLEVKAKGYDPFES
                     DVDVHFQKVSPVVVRLLQSGETVGTGRTIYKERTPIYTKTWFIATVAVVAVGVGILAG
                     RNIGSVPCYINGDRSMPCD"
     gene            21147..21575
                     /locus_tag="VMZ53_24595"
     CDS             21147..21575
                     /locus_tag="VMZ53_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31713.1"
                     /translation="MSWARVFVIAALVTCAAACSSVTTTRVQPESVAVGPHMRPIAVV
                     HAQVTSAYFLFLAIPGHVSLDYVVNRMLIHAAKELGADKVVDIKVDITPGDGIWTFRK
                     ILGWRSAQASGIAVVMEDEAAAPGAPATATPAQPQPAPTK"
     gene            21590..22513
                     /locus_tag="VMZ53_24600"
     CDS             21590..22513
                     /locus_tag="VMZ53_24600"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HUS31714.1"
                     /translation="MPLDVFDRRLILVVGKGGVGRSTVAAAIAGQCAARGRKTLLFET
                     NANDRFGTYFDKPAVGTEVTQLAPNLHAVNTNPAHALEEYGLMILKFRSVYEMVFENR
                     VTRAFLRAIPGLDDYALLGKAWFHTTEEKRGKPVWDTVVFDMPASGHSVTMLRIPWVI
                     TDTVPEGPLTRDARTIKELLTDPKRTAAVLVTLAEEMPVNEAIELEAKLSGFGIAPQH
                     VVCNQVYPAHFAPGAPVTKVVDTLAADPNLPSPLREVTEHATLSRDRRKLNEHYLTEL
                     RARTKTPVQELPMLFTPALDAAHVKALGDRL"
     gene            complement(22514..22795)
                     /locus_tag="VMZ53_24605"
     CDS             complement(22514..22795)
                     /locus_tag="VMZ53_24605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31715.1"
                     /translation="MVSVTARKIRELGSERPGGASGRIYTGYLISYPYIGRGMVIFRD
                     PHGHRMITTPVKRVLGEPGGKMIYVETENSVYRLEIHGEPLFPMRQTGE"
     gene            22867..23760
                     /locus_tag="VMZ53_24610"
     CDS             22867..23760
                     /locus_tag="VMZ53_24610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS31716.1"
                     /translation="MAEPTIDALYEQVRTAVPVAAGDVPAELLPVGPGIRVLALRTPT
                     LPPAAHTNVYVVGPDAGPQLVVDPGSPYPDQQAILDSALEREADAGRPIGGVLLTHHH
                     GDHVGGAAALAARFGVPIAAHRRTAERLRGRVDVARELGDNEILDDITCVFTPGHADG
                     HLVFEKAGAQIAGDMVAGIGTILIDPSEGDMADYLASLARMLERAPSLLLPAHGPPIT
                     DAAGKLREYIAHRTMREDRVVAALTSRADASVDELVPDVYADTPRILWPLAGRSLRAH
                     LDKLVKQGRAREVEPGRWTAR"
     gene            23783..25216
                     /locus_tag="VMZ53_24615"
     CDS             23783..25216
                     /locus_tag="VMZ53_24615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS31717.1"
                     /translation="MGLTFDTLAEVALARDMLRRWYGLELGLSAADGSDYDRTSHETC
                     ESVRKHPSGGCAAALKEAASVLAGRKGAAAVVHRCHAGLSVVAAPVRSTSGLTAIVYS
                     TGGRTNAEATSSIGTLLSLGADEATAAAARAPELSDADIAHVKDLVEAAAQAVERALP
                     TVIPPTTFTHPFTEMVGDAPAIREVVKLLTKVVKSEATVLIHGESGTGKELIARAIHY
                     HGPRAKKTFVVQNCSAFNDNLLESALFGHVRGAFTGAVKDQKGLFEVADGGTFFLDEI
                     GDMSAALQVKLLRVLQEGTFTPVGGTKPIKVDVRIIAASHKDLQAMVAHKQFREDLFY
                     RVNVLKITVPPLRDRVADIPRLAAYFVTKHWKFPGPAPRLSQSALARLVAYPWPGNIR
                     ELENEIERMLVLAADAQELTASMVSQRLGGQSVGTPRTLRDALAIAEAGAITESLGRH
                     SGDRDAAAADLNVSRAYLEARVAALGL"
     gene            25222..25431
                     /locus_tag="VMZ53_24620"
     CDS             25222..25431
                     /locus_tag="VMZ53_24620"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HUS31718.1"
                     /translation="MTSAKNAKARCPTCRGVSLKEANKVFPFCSNRCQLIDLGRWLDE
                     DYRVPEESDASGGGIEGIPRTDDER"
     gene            25501..25887
                     /locus_tag="VMZ53_24625"
     CDS             25501..25887
                     /locus_tag="VMZ53_24625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31719.1"
                     /translation="MRPSVDEPFDPIALAILLGLANTIVIAFGLAVAIPERGVAGAIF
                     IFGFIPGMMIGAIEGGIARLTARTPPWVRLILLTPIPVGFVLLMATSLHFRASSTGAA
                     IPTLVAVLILERHSRKRPLVPPARVA"
     gene            25897..26559
                     /locus_tag="VMZ53_24630"
     CDS             25897..26559
                     /locus_tag="VMZ53_24630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS31720.1"
                     /translation="MVEHRVLDHLGLAAADYDAAIRRYIPHYDALIATIVELVDGDVT
                     DLGTGTGALAAAILTKPSTRVRLVDIDPAMLSTAASRVAPFGDRATCVRASFDEGLAP
                     CDAVVASFALHHVPTLEAKRSLYARIHAALRPGGILAIGDVTIHDDGPANTTMMNAWA
                     AWMSDHGIARADANALFAKWAGEDRYFSLADELATLAAAGFSRPDCFWKHGPSTVFGA
                     YR"
BASE COUNT         4543 a         8738 c         8867 g         4578 t
ORIGIN      
        1 gagcaccgtc gtcggcgcga gcatgcacgc ttgcttgccg ccttgcaccg ccttgaacac
       61 ggcgcgcagc gcgacttctg tcttgccgta gccgacgtcg ccgcagacga ggcgatccat
      121 cgcgcgatcg gtctccatgt cgccgagcac ggcattgatc gcgtcgagct gatcgggcgt
      181 ctcgtcgaac gggaacgtcg cttcgagctc gcgatacgaa tcgtcgggcg gcgggaacgt
      241 gtgacccggc agcgactttc gctgcgcgta gatctgcagc agctcctcag cgagcacgcg
      301 gacgtggcgc ttgaccttgc tctgcgtggc ttcccacgtg acgccgccga gcttgtcgat
      361 cttcggcgcg tgaccttcgg cgccgacgta gcgctgaact tcgccgaggc gatagacggg
      421 cagatacagc gtgccgccgt cgtactcgag gtggagcgcg tcgatctcgg tcgcctttgg
      481 tgtcggcgcg ccgacggctg tcgtctgcaa gatcgagacg gtgccgaccg cgatcttctt
      541 gaggccgtga tagcgcccga caccgtgctt ttgatgcacg aggtagtcgc cggcttggag
      601 ctggctccag tcggaaacgc cgccgagcag cgcatctttc gcgcgcttct gcttgttcga
      661 cggatggtga acgcgctcgc cgaagatgtc cgtcgcggtg acgagcacga cgccatcgcg
      721 aggtgacgcg aacgaatgcg tcggtgcgcc gggaatgagt gtgacgcccg atgtcgcaag
      781 cggtgcctca cctgcggtcg cgatccgcac cgaaataccg cgggcctcga gcacgccgtg
      841 tagtcgatcg acgcgactct gcgaatcgca tgcgatcgcg acgcgcaggt ccttccactt
      901 cttcagcgcg tcgagcagcg gcgcaacatg ctcggtatcg ccgaacgtac gcgcatgatc
      961 gagctgctgg cggagcacgc gcaggttgtc gacctccacg cggagcttcg tcgcatcgga
     1021 gtcgccgtgc acttcgagcg atgacagctc gatgcggagc ggcgccgaga acgcgccgag
     1081 ctcttcgacg aagtgccgcg ctgggacaaa cgcgagctgc ttctgatcgc gctgctcgta
     1141 acgcgcctct tcgtccttcc acagatcgct gatgacccgg cggcacgcat cgggatcgac
     1201 gacgagccac cgcgcatcgg cgggcacata cgtctcgggc gcgacgagct cgtcatgaaa
     1261 tgcaggcgtc agcccctcga tgccgacgaa cgcgtcgccc gcctcgagct gctcgatcgc
     1321 tcggcgcgtc gccttcgacg gaaatgcgat ctcgtcggcg tactcgcgca ggcgcgtgcg
     1381 gacttcgcgc gtgccggtat tgatcgtctc gcgcacggga tgcagatgca cgtgctcgac
     1441 ctggcgcaac gtccgctgcg attcggcgtc gaaccaacgc aacgactcga cctcgtcgcc
     1501 gaacagctcg atgcgcacgg gatgcggcga cagcggcgca tagacatcga tgacgccgcc
     1561 gcgcacggcg aacgtgccgg gctcgtcgac gacgggcgtc cgagcccaac ctcccgcaac
     1621 gagcagcgcc gccacttgat cgcgatcgat cgtctcgccc ttcttgatcg tcacgccgcg
     1681 tgtcgcgagc tcgcccgccg gcacagtacg ccgtgcaagt gcctccgcgg acgtgacgac
     1741 catccgcggg cgaagcgcag gctgggtcag ccgatacagc gtcgcaacgc gctcgacgat
     1801 gcacgcgcga tccggcgaca gctccgcgta cggcgacacg tcgatgccgg gcagcgacgc
     1861 caccgcatcc agctccgttg catcgcgctg gccgccgaga aaaaatcgga tgtcgctctc
     1921 gagctcgcgc gcggcggcat catccgcggc cacgacgaca acgagcgcct cgcgagcagc
     1981 aagctgcgca gcaagccacg ccgcccaacc gggcgtcgat ccgtacgcga tcgtcgcgcc
     2041 ggcatcgagg gcatttcgca ggtcgctcca catagacggg cgcgcaagct agcacgcggg
     2101 tagctcgtga aaagcggtca ccgcgaacgg cggcggcgca ctactttgcg aggacgacgt
     2161 cgagctcgag cggcagcttc gcttcgtttt cgatcggcag cttgatcgtc tcgccgcccg
     2221 gcgttccggt ctttgcgagc acgatcggtg ctccgagacc gaccagtgat gcttccacag
     2281 catggccggc gacgcgcttc ttgatcgctt cgagatcgac cggcgagccg agcacgactt
     2341 gaccgctcgt tccttgttgc tctgcgatct cggccgttgc gatcacgatg ggcgcgccat
     2401 tgcgcagcac gatcttcgtc ttgccgaccg gcggtgcatc gagcgcggct gcgtcgcctg
     2461 ggaggcgcag catcggcgtg cgcgtgccgt cgcgaacttg gatgacctcg acggtgtcgc
     2521 ctgcttcgag cttgaagacg acgtccttgt ccttcgccat gtcctgcagc gtctgtgcat
     2581 cggtaaggat accggcgcgc agcatcgacg acgtcgccat agcttcggcg cgcacgagtg
     2641 ccgccttcgc atcggcgtcg aacgtcgtac cgatgaattg cgcggccgct tggagcggca
     2701 ggcgatgttg atccgacgcc gaattggctg gcgtggtcgt cgttgccgct ggcttgctct
     2761 tgccgacgag cacgacggcg acgacgacac ctgcgatggc gacaccgacg ccgatgaaga
     2821 tcggagccat cgaccgccgg cgcacgcctg cggtgaccgc gcgagcacga cgaggcggtg
     2881 ctgcgacggg ttccggctcc atgatcggtg gcggtgcgag ctcggtcttc tcgtccgaga
     2941 tctcgatctt cgcgatctcg atcttcggga tctccatgct cggagacgac cggatgatca
     3001 tcgagcgcgg cgcgatctca ccggtgtgaa tcggctcgtc tgcggtcggg tttgccgcgc
     3061 gccgacgccg ctgcttctgg acgacctccg ccgggacggg cgtgggctcg tccgcggtca
     3121 tctcgatctc gggagactcg tcgtcctgcg acattctgtc gtcccgagct tacctccatg
     3181 cggggtccgg gaggggcccc taccgttggg gcgaattgca gttatttatc cgaatggtcg
     3241 gcgcgaagct cagtagctct cggacgcatc gcccgacgcc gccgaagcgt cgctatggcc
     3301 aagaccgcga acagcgtggc gccaaatccg gtcattcctg ccaatgcgat catggcccac
     3361 gggaaggcgg gtcgcgtctc gaggaccacg atctgaggcg ggggtggcgg agcggccgtg
     3421 ggcagcgtca gtacgatcgg cggaggcggc gcgagctgcg gctcgacaac gggaggcggc
     3481 ggtgccgggt ccgccgtgat gagcacgccg ttcggcgtct cgtccgcgat cttcagggat
     3541 ggcgagacgt cggtaacttc gcagccggcg agcatcgcga ggttgcgata caggcccggc
     3601 gacatctgca cgtacgtgta cgcgaggtcg acgccggtgc cgttgatctg cagctccttg
     3661 aagccgccat cgggcgtgcc gatttcgacg cttgtcgccg cttcggggat ccccggcgcc
     3721 ttctcaccac acggctcgca gaacgcgatc accttgggat ggctctcgac gatcttcttc
     3781 gccttcgtcg ccgccttcgc gtcgatccac gcgcattgat ccgcatacgc cgttcccgcc
     3841 atcgcgagga ccatcgtgat cgctagagtc ttcatatcgt cctccgtttg tagtgagcga
     3901 cgccgagcag aagtgcaacc acgaccgtgc acgccgtcac gacgagcagc gcgtggtggg
     3961 cgaactcccc acgtgcagca gctccattcg cgagtggtaa gccgagcgtg atcacgagat
     4021 acgccgcgat cggaatcgcg aaggtcgccc tccgcttgcg ggcttcgatc gctcgtgcaa
     4081 gcgcgaacag cagtaacagg ccgccgaggt acacgacaat tccctcgagc cgatgcgcct
     4141 ctgcgcggtc gaggtcgccg acgcgcagat gtccgcgatg catcgcgacc gcgatcgaga
     4201 ttcggatcgt gttgaccacg atcgtcgcga gataggtggc gaccgcggcg aacgccaacc
     4261 gaacggcgag cgcacgcgga cccgtgatcg cgctgaggga tccgaacacg agcgtgacaa
     4321 acgcggcgag cgcgaagttc atgcccgcgc acggcggcgc gatgatgaaa ccgatcttgt
     4381 agtcggcgta gccggcgccg aattcgtaga cgaagttgcc gccggtcagc cacgagacga
     4441 gatgcgcggt gggcgcgagc aggaagcgca ggtcctcggc cgacgccgag cggtagaact
     4501 gcttgccggc gatcacggcg gcgagcgtga tgacgaccgc cgcgatctgc cagcgatgct
     4561 cgcggacgaa cgtcacttca cgcctcgcgc tgcacggcgc tgcgtgagga tcagcaccgc
     4621 accgacgagg acgagcagga taatgaggcc cggctcggcg ccgacttcgt tgccgctctc
     4681 tgcgaccgcg aggtcgctga cgccttcggg catgggcagc ggctgcgtga catccgtcga
     4741 gcccgccgtg gtgcgcacga tctgctgcac cgcgatgaac gacgtgtact tcgtgagcag
     4801 gttgtagtcg aggccgagct tcgtgacggc ttccttgttc tcgtcgccga acgtgaagtc
     4861 cgagagctcg ctcaccttgt tgcgggccca cagatacgag agcgcctggt tcgatgcgtc
     4921 ggccgagaac tttgccggct cgacggtggc gacgaagcgg ccgcggcccg agacgccggt
     4981 cagcgtgatt ttgccgctcg ccgtgccctt gtacttgccg aacacgacga cgggacggtt
     5041 tgcgaacacg tcggggatcg acttcgacga caagtcgtat gcgttgaagc catcgaactt
     5101 cacttcgacg ttcgtcagca ccggcgactc gacatactcg cgaaacttct tcgcggcatc
     5161 gggcgcctgc ttcgggtcga ggatgacgaa cggctcgcct tggccggcct gtgcgacgcc
     5221 gtcgacgagg tggcgattca ccgagctgcc gatgccgaac gagaagacat tcgcttcgcc
     5281 gaggtgcgcg cggatgtgct cgaacatctc ggcctcttcg ccgatgtacc cgtcggtgac
     5341 gaccacgaag ctgcgcgaga gatccttgct gagacgcggc agcttcatcg cacgctgcat
     5401 cgcgggaagc agctcggtgc cgccgccgcc ttgctcgcgg tcgatgaagg agatcgcgtc
     5461 ggcgatcttg tccttctcgg cgggcaccga cttctcgctg tagaggctcg agccaccgga
     5521 gaacagcagc acgttgaagt gatccgtcgg gcgcagcttg ccgaggaggt cgcgcatgag
     5581 cgtcttcgtc gtgtcgagcg ggaagccgtt catcgagccc gagacgtcga tgatgaagac
     5641 gtactcgcgc gccggaatca tctcgagcgc cggacggtgc ggcggctgca ccatcatcaa
     5701 gaagaagttt tccttttggc cgggaaacag gagcaggccc ggtgtgatgt cgtcacccgc
     5761 gaggcggtag cgcaggacga agtccttgtt gccggtcgcc gagccgttca cgagcgcgat
     5821 gtttgcggac gtgccggccg aacccatcga cgttttgatc gtgtgcgacg gcgagtcgat
     5881 cgattggatc ttcatgcccg ccgcaaccga cacattgatg tcgaacgcgt agctcggctg
     5941 ggccccggac ttcgtgtacg gcgacgccac gaactggttc gtcggattct tcgcatcgag
     6001 gttatcgctc gcgtagcgtg ggccgacgac ggtcggataa tcgagctcgt acacgccgtc
     6061 cgtcgggacg agcagctcgg tgtacttcag ctcgacctcg atgcggtcct tcgggaggat
     6121 gttcgcgacg ttcatcgaga agacattggg ccggtcttcc tcgagcagcg aggcggtctt
     6181 gcccgccgct ttggcctcct cgtattcctt gcgcgcttcc tggcgctcct tgatcttcgc
     6241 gacgatgacg cggtcgccga tcgtcatctt cattccgtag accgccgctc tcgtcgatgc
     6301 cgggaataca tacttcgcgc tgatcgtttt ctccccatcg ttcctgtaca tctgcttgac
     6361 cgtcacatcg gcgatcacac cgctgatgtg gacgtcggct ttcgtactct cgagcggcaa
     6421 cgcctcgacg ccgggcgctg cgccctcgac gaagaagaag ggggagagcg acttgtctgt
     6481 gttatctgcg tgaaccacac gcggcacgac aagcagggcg agaacgagcg caagaatcct
     6541 catgacgcgc gatagagcaa cgacgatgcc actcgcatct acgcggaacg tgatgacgcg
     6601 cggccaacga gacacacgct atgctgacca cgtgacacgc ttcgcgatcg tcgcagcgct
     6661 aactgtcgcg ggcgcagcgc atgcggatac cccgcacgac gtcgcacgtg ttcgtgggct
     6721 gaaggtcaag aagccaatcg cgcaagagat cgtcgatcgc gcagatctgc agtcgcacct
     6781 tctgagcaag aagacggcgg ccgagctcga ggccgaagcc atcgcgcgga cccgctgggg
     6841 cctcgctgcg ccggtgacga acgacgatca agtcaccggc acctacgatc cggcgacgaa
     6901 aaagctcgtc ctcgtgacgc cggtcgaaga gttcgtgctc gtgcacgagg tcgagcacgc
     6961 gctgcaagac atgcacttcg atctgtcgaa agtcgaaggc ggcaccggcg atgaagccat
     7021 cgcgcgccac gcgctcgtcg aaggcgatgc gatcgtgacg acgctcgagg tgctgctcga
     7081 tcgcaaaggc gtcgatccac cgtgggataa cccgcaggtg gtcgcggagc tgattcgcgc
     7141 gttcgatcta cccgtcgacg agtcgtacaa ggccggcttt gcgttcgtcg tcgggctgcg
     7201 cgcgcagaag ccatggtccg cggtcgacgc cgcattcaag cggccgcctc gatcgaccga
     7261 gcaggtgctg catgtcgaga agtacctcgc cgacgagcaa cccgtcgcgg tcgaaacgct
     7321 ggtcgtcgcc gacgcgcgaa tcattcacga gacgacgtgg ggcgagctcg gcgtgcagca
     7381 atttctgcgc gcgcatggca tcgacccgca gaccgcgaag ctcgcggcgg cgggctgggc
     7441 aggcgatcgc gtgacgaccc tcgagaagaa tggccgcgtg atcggtctcg cgcgcctggt
     7501 gttcgacacg ccggcggatg cgatggaggc cttcgacgcg atcgagcgcg cgatcgatga
     7561 cagccaactc gccgcacgcg ccgagaattc cgaggcccgc acgcgctggc tcgcgctcga
     7621 cggaacggtc tccacgctcg aacgcagggg cgaggagatc acgatcgagc tcgctattcc
     7681 gctcgcgcaa atcggttcgg cgttaccgtc gtctagatga ttcgcgtaac tgctctgatc
     7741 gtggcgatga gcgtcgccgg ctgcggtctc acgatgaccc aggggcccaa cccgtcgcag
     7801 cccgccgacc aacgcccggt gtgcaccgag accttcgcag catcacgccg agacggggtc
     7861 ggggcgatgg tagggctcgt cgcgatcttg tttggaacca tcgcggtaaa gggtgccgac
     7921 aacacgacgg tcggcgtgcc gcttctcgtc ggcggcggcg tcgtcatcgt cggcgcatat
     7981 atctccggcg gcgtcgggta catgcgcgtc aagaagtgca agaaggcgat cgaggccttc
     8041 gagcgccgca ctgtttcggt gccgccagcg acgccgtagc tcagttgctg cgctgtagcg
     8101 acagcggctt gccgtggcgg agcgggacgg tgaacacggt gccaagctcg ctagcgactt
     8161 cgaacgcaaa gatgacgtcg ccgccgtgct gcggatcctc ggggcagaga tcgacgcgct
     8221 tgatgccgaa ctcgtccggc tcgtagagct gcacgccgtt gcgcgcggtc tcgcggagcg
     8281 tgaacttgat gacggactgc caggtctcgt cgagctggcc gcgcagatcg agccacgtgc
     8341 ccatcgcctc tgccgacggg ccgctcggat caccgtcgag cgcgacctcg atgacttggc
     8401 ccgcgaagat gatctgctgc gtccaccggc ggcgctcggg attccacgcg agcggcgttg
     8461 gctcgggggc gcgcggctcc gagaccggct cgggaaacaa gcgcggcaag atcagtgcag
     8521 ccgcgagcag caacacgacg ccgagccaga accattcacc gttcatcgag cttccgctcc
     8581 caggagatag cgctcgagac cgacgaggcg agacacgtcg tggacgtcgt cgcgcatgag
     8641 cgggacttcg atgagcagcg tgttggctcc gcccgcggaa cgcagctgcg cgatcgctgc
     8701 gcggtcggcc tcggccagcg tttcgagctc gccgtgtgct gcgaacagcg catgcgcggt
     8761 catcgtgcgc gtcgtccccg agataccgag cgcagcgacg cctggatgag ccccgagtgc
     8821 ggcagcgagc gttggctcgt cggcacgaat cggcagagcg gggtgcacct tgttgacgac
     8881 gaagccgacg aacggcatcg acgcttcgac gaggcgctcg tggaagaaca gtgcttcgcg
     8941 cactgccatc ggctcggggc tggcgacgag cacgaacccg acgcgtggct tgcgaagcag
     9001 cgagaacgtc tcttctgcgc gcgtgcgaaa gccgccgagg atgtcgttga actcggtgaa
     9061 gaacacgccg aggtcatcga tgaactgcga gccgacgaac ttcgcgaggc gcttgagcac
     9121 gaacgcgccg gtcttaccga cgaacgacca gccggagccc gagccgccct gcagcttgcg
     9181 gaaccattcg atcgcgggcg agtcgatcgc ctcggacagc ttgcgaggtg catcgaggaa
     9241 gtcgagcgcg tgcgcggtcg gcggcgtgtc gacgatgatc gtgtcccact ggccgctctc
     9301 gtcgaagtcg tgcagcttcg ccatcgcggc gtactcttgc gcgcccgcga gcgagcctga
     9361 aatctgcgcg tagacggggt tcgcgaggat gcgctttacc gctgcgggat ccttcgcgtg
     9421 acgatggacg acctcgtcga acgccatctt ctgatcgagc atcatcgcgt gcagctcgcc
     9481 cgtttgtgcg ggagcgccgg tgcgcacgag cgcacggtcg acttcctgga cctggtggcc
     9541 gagcgccgag aggccgagcg agtttgcgag ccgcttcgcg gggtcgatcg tgaggaccaa
     9601 cgtgcgcttg cctcggcggg cggctgcgag agcgagtgtc gccgccgtcg tggtcttgcc
     9661 cacgccaccc gagccgacgc acacgatgat gcggcgctct agcaatacgt ctacgaggcg
     9721 aggcacgtgg cctgcggata gcatataaga gggcgcacat gaagcgcgag ctgatcgtta
     9781 ccgcagacgg acgcgatcga acggtcaccg tcgaggggcc gctccccgat ggccgatttc
     9841 gcgtgacgat cgacggcacg gcgcacgatg tcgacgggaa ggccattcgg cccggcacct
     9901 ggtcgctcgt gatcgcgggc cgcaactacg tcgtcgacct cgacaagcgc cgcaccggca
     9961 tcgcggcttc ggtaggcgcg agtgaggcga tgctccaagt cgaggacgcg ctgcatcgca
    10021 ggctcgcggc cgcagcaagc ccacgcgaca aagcccgcgg cgagacgctg cgcgcgccga
    10081 tcgcgggcaa ggtcgtcaag ctcctcgttg cagtcggcga cgacgtcgtg ccgggtgcgc
    10141 ccgttatcgt gctcgaagcg atgaagatgg agaacgagat cgtgtccgag cgcggcggca
    10201 aggtcaccgc gatcaacaag caagcagggc aggcggtcga tacgggcgac gtgctcgtcg
    10261 agctgtctta gcgccgctac gcgccgagct cgtgatcctc ttctcgcgcc gacgggaact
    10321 tgagcggcac gctgagggca acaagcgttc cgcgtggctc ccgccgccga atggtgcact
    10381 cgcctccaca gcgtcgcgcg cgctcgcgca tcccgagcag tcccaatgac gagctgcgcg
    10441 gtgcgatcat cggcacgccg acgccatcat ctgcgatgtc gagcttgagc atgccgcgct
    10501 ctagccacag atgacactct acgaacgtcg cctgtgcgtg acgcgtcacg tttgtcagcg
    10561 cctcttggaa gatgcggaag acggcggtcg ctaggcctcg ctcgagatgg aggtcggcga
    10621 catcggcctt gacgtgacac ggcgtaccgg tgcggcgcgt gaactcctcg gcttgccact
    10681 cgagcgcagc gcgtaacccg agatcgtcga ggatgccagg gcgcagctca gcggagatgc
    10741 ggcgcacgga gctgatgatc tcgtcggtcg tcgatgccat ctcttcgagc ttcttcggga
    10801 gctcgtcgcc gtgaccgttg ccgttgcgaa gtcggcggca cacgagcgcg atgtcgagct
    10861 tgagcgccgt gagcgcctgg ccgagctcgt cgtggatctc gcgggcgatg ttcgtgcgct
    10921 cttcttcgcg cgcttcttcg atgcgaccgg ggagcttgcg gagctggtcg tcgagctggc
    10981 gacgctcgat cgcgatgccg gcgacgtgcg cagcgcgctc gatcagctcg cgagacgcag
    11041 cgtcggggat gcgctgctct ttgtagtaga cggcgaacgt gccgatcacg cgtccgtcgc
    11101 tcgcgagaat cgggaacgac gaacaggcga ggaggccgta cggctcgacg agatggcgat
    11161 acaggagcca cagcggatcc gtggcgatgt ctcgaaccag caccgcttcg cgccggtagg
    11221 ccgcggtgcc gcacgagcca gccttgggcc cgatcgcggc gccgtcgatc acgcggttgt
    11281 atgcgtctgg aaggttgggc gcggcgccgt gcttcaagtg cgcgcctcgc tcgtcgagca
    11341 gcagcaccga cgcgatcgtc cgcgggctca gctcttcgat caacgcgacg atgacggcca
    11401 gcacttcctc gagcggtgca ttcgcggcga gcagctcgag agcttggcgc tcaccggcct
    11461 gcacgcgttg cacgcgctcc cgcgctttgg cgagctcggc ctcctccgtg atgtcgcggc
    11521 atgtgccgat caacgtgacg ggagcgcctg cgtcgctctt gatgacctcg ccgatcgtgt
    11581 cgagaacccg cacctcgccg ctcggccgaa cgacgcgctc gagccacgcg aatcggcctc
    11641 cacgctgcat cgctgccgtg acctcggcct ggatgcggtc gcgatcgtcg gggtgcagag
    11701 cgccgaggaa cacctcgagc gtaatcgtcc gttctcccgg tacgagaccg tagattcggt
    11761 agagctctgc cgaccaggag acgacccccg acgccagatc ccattcccaa ctgccgacat
    11821 gcgtgatggc ctgcgcagcg tcgagctgcc tcgccatcgc ttcgctcttc ccatccgcgc
    11881 ccatagctca caacgctacg ttagaatgag gccgcgtgca tcggatcctg ctcgtcgacg
    11941 accatgtcgt agtacgccgc ggagtgcggg cgatcctcga agaccatttc ccggggattc
    12001 acgtagccga ggcgaacaat ggcgacgaag cgctcgcggc gctcgcccag ccgttcgacg
    12061 ccgtcgtgct cgacctctcg atgccgggcc gcagtggctt cgatctgctc tccgagatca
    12121 agcatcgcta tccaaagctc ccggttctca tcatgagcct gcatgacgag gagcaattcg
    12181 cggtgcgtgc gttgcgcgcg ggagccgctg gctacctgac gaaagccgcc gcttccgaac
    12241 agctcgtcgc cgcgatctcg aagatcacgc gaggcagcaa atacatcagc gacacactcg
    12301 ccgaacggct cgcgacagac gtcggtggcg accacatcgc gagccggcac gagcgcctct
    12361 cggatcgcga gttcgacgtc atgcgcggca ttgcatccgg cagcagcgtc agcgagatcg
    12421 cggcgcagat gcacctcagc gtgaagaccg tcagcacata tcgcacgcgg ctcctcgaga
    12481 agatggggat gtcgacgaac gcggagctca cgcgctacgc gatcgaaaac ggcctggttt
    12541 agttgggttc ccacatttgt aggacggaca ccgacgcaat gatcagcgga gtccgactgc
    12601 aggatcctcg gatccccgac ggtctaagcg ttcgcgatga cgaacactgg ctgcatgaag
    12661 gtgctgatcg tggatgggtc gaagcagaag cgcagcaacc tcgtcgaggc gctcggcgag
    12721 ctgacaaacg tggtcgtgca aggcgcggtc tcccacgtcc gaacagccat tcgagtcgtc
    12781 gccgacgcga cccccgatgt gatcgtcacg ggcgttgtcc tgccagatgg ggagggcact
    12841 cacctgatcg agcgcgttcg ccggatgacc gcgccgccct ctatcgtcgt ggtggcggat
    12901 ccgggctcgg acgaacaacg ttctcgctac ctcgcagcgg gcgccgaccg ctacgtcgac
    12961 tccgacgacg tcggcgaagt gcaagcggcg gtcgccgggc tccgcgcgcg cgaaggtgta
    13021 tcgccgaccg agtcgcagcg gctgctcggc cggatgacga cgggcgtcgt tcacgacctc
    13081 aacaactatc tcgcggtgct cgatggctcg ctcgagctca tgcatcgcaa tccgcaagat
    13141 ctcgctgagc tgtggccgcc ggttcgcacg acgatggacg cgatccaaga tctggtcggc
    13201 acgctgctcg cgtacgcgcg cggtggcacc ccgccggtcg aggagctcga cgttggtgag
    13261 gtcgtacgcc acgcgatcca gatcggtcgc cgcatggtcc cgacgaatgt ggtcctaacg
    13321 gtcgagatcg cgggcggcct acgtcgcttg cacggtattc gcgccgagct gcagcaaatg
    13381 atcctcaatc tcgtggtcaa cgcgtgcgac gcgatgccgc tcggcggcga gctcaacgtc
    13441 gccgtcaaag gcgcgacggc gcaagcggtc ctcgtcgagg tttccgacac gggaagcggc
    13501 aagccattca tgccgctcgt ggcaggtcgc acgcagtcga cgaagaaagg ccgcttcggt
    13561 atcggcctcg gcatgggcat cgtccacgcc gtcgtcgatc ggcacaaagg tgcgatccgc
    13621 gtcctcgaac gcgcgggcgg cgggacgctc gtcgcagtga tgctgccaac gcgcgcggag
    13681 tgataccgtg atcatccatg aggtggcttg tcgtggtcct gatcgtcgcg actgcgtgcc
    13741 gtgcggagcg aacgaaccac tgcccgagca acgtgaccag caactgcatc agcggcgagg
    13801 tctgcagcat ggatcgacag cgtggttgtc aggtttgcca gtgccgaccc tgggacggga
    13861 ccccgcgcag caaggatccc gacgacgaag ggacgccccc catcccggtt cactgagcta
    13921 ctacggttcg tcgcactcac cggccgcgcg cacacgcgag atgtgagcga gcacagcgag
    13981 gtcgatctcg gcggtgtgcg agccgaatgg atacgcgtag gtcgtcggcg gatagccgag
    14041 ctgctcgagg ggcgtgaacg acggcaggac ctcgtcggcc acgtatgcgt cgacgccgtt
    14101 cgccgcgacg tactcgaccg catcgacgtg agccacgctg tgtggctgga ggtcgtgacc
    14161 gagcgaggcg agcgtcatga cgtcggcgcg ctgcgcgtcg gtcatcgtcg tccagcgcga
    14221 gacgaacagc gttattcggg catcgtgtgc ggccagtagg tcctgcgcgg tgagccagag
    14281 ctcgggactg ttgtcgtcga acgcgaaggc gagggcgccg cgtggcggac cgtcgagctc
    14341 acgaaacgtg atcgtcggga gaccgcgttc agcggcgctc gtcagcgcgc gctcgatcga
    14401 tgccagtgac accgtaatgc ccggcttgtg cgcgtggagg atcgcaacgg agccgtgcgc
    14461 ggccgcgttg tcgagttgct cgtcgacctt gtcccatgcg acctcgtggc tcaggtcgtc
    14521 gatcggcacg ctgcaaagga cacgatcgcg gtcccagtga tacgtgatgt cctcgccgcc
    14581 cgagcacgct gcgagcacga gcagcagtgc ggcgaaccgc gtcacggcat cgcggcgcga
    14641 atcggcggcg tcgccgcagc cggtgcaccc ggcaagcccg gcgtgcgatc gcgcgggatg
    14701 tgatagcgcg tcaggatgcg aggcgtcgct ttgcggacct cgtccgcgtc gagcatgatc
    14761 acgccgcccg acgtcgtgtt gatctcgacg acgccacgcg cggcggatag ctgctggacg
    14821 aagtcgacca tgtcgagcgg cgctttgccg ttcagcgtcg caacggcctc gttgtagagg
    14881 tgcgcgtagc ccacgttgat ttcgtcggcg aggatctgcg tgaggatgac gacctcgtgc
    14941 tgctcggcgg tgcggtagcc gaggtagtag tagttcaaga actccttcgg agctttgttc
    15001 caccacttgt cccacgtcgt caggtagtcg cgcgtcagcg tctggaacac gaggccgccg
    15061 tagacgaagt actgcggcgg ttggtcgtag cgcgagcgcg gaacgagcgg ctgccacgcc
    15121 ttgagctcga cttcgacggt cttgagcttg ccggcgcgct tgatgtcgag cttgatcttg
    15181 tcgccgatgt attggtagcc gagcacgacg tcgtagcgcg tgcggtaccg gttcatgaat
    15241 tggacggtgc cgttgttcgc gatcgcgagg ccatcgatcg acgtgatgac gtcgcgctct
    15301 tgcaggatgc cgtaggccga gccgccgtaa tcgacgtgga gaacgacgac gccgcgctcg
    15361 ccgtcaccga ggccgagctg cttgcgcagg agcgggttct cgagattttg cgtcgtgatg
    15421 ccgagcgccg gaatctcggg gcgcttgccc ttctcgacgc cgtcgaggaa cgagcgaatg
    15481 atcggcggcg ggaccatctc gccgatgttg tcgacgccgg tcagcttctg gaatgcgatg
    15541 ccaacgacct tgccgtcgcc gaagacggga ccaccgctgt tgccggcgtt gatcgcggcg
    15601 tcgacggtca cggcgagcag gtggcgctgg ctgtggctgt accgctggac ctcgatgcgc
    15661 gagacgacgc cctcggtgat cgagatttct tcgccgccca cggggtagcc gacgaccgct
    15721 acttcgtcgc ggaggcgcgg catgtcgccg acctcggcag gctcgatatc cgcaaggaaa
    15781 tcagctggtt cgaccacctc gaggagcgcg agatcgcagt cgtggctgac ggccttcacc
    15841 cgggcgatgg ccttgtcggg agccgacacc ttttggatct gcatgaacgt cgcgttggcc
    15901 acgacgtgcg cgccggtgag cagcaaacca gggccgatta cgacggcaga accggtgctg
    15961 ttcgagggag tccgcgcctg ccatggtcca tcgaagtccg ggtcctggct cgttgcgaag
    16021 acgcgaacaa cctcagggtg cgccgacacg atctaagggt accgtgcacg catggacgca
    16081 gcaaagtgta gtgtccaccc gatcttctaa cgcaccggaa tgcacgcact tcaatctata
    16141 acacctgaac gtttggccgc cctcgtgccg gtcatcacgc tgggtgaagc tcgtaagttg
    16201 gtcgcccagg tgcaccgcgg cgagccaatc gcgcctacct ctgccatccg gcgaacggcg
    16261 gcagaggcgg tccgcgcgat cggggaggtg cgcacgctcg aggtcatcgc ggagactgcg
    16321 agcgcgatcg atccgttcgt gaagtacctg ctgggatcgg gcgatgcgcg cttcgaagca
    16381 gtgcgcatcc cgctggagca tcccgatcgc ttcagcgtgt gcgtcagctc gcaagtcggg
    16441 tgcgcgctcg cgtgtgcctt ctgcgcgacc ggtcgcctcg gcctcacgcg caatctcgaa
    16501 gcgtgggaga tcatcgagca ggtgcgcgtc gttcgtgcgc ggctatctcg cggccgcgtg
    16561 cacggtgtcg tgttccaagg catgggcgaa ccgctcgcga acctcgacga ggtgctcacc
    16621 gcgatcgacg tgatgtgcga gccatgcgcg ctcggcatcg acgcgcgtgc gatcaccgtg
    16681 tgcacggcgg gccttcccgt cggcgtgcgc cggctcgcac gcgaagcgcc gaaagtccgc
    16741 ctcgggctgt cgatccatac cgtgctgccc gagcgtcgca agtcgctgat gccgatcgct
    16801 cgcgcgcatg cgatcgacga gctcctcgac gctgccgtcg agcacgcgcg catcacgggc
    16861 ctctcgccga tgtgggcggt cacgctgctc gatggcgtca acgattccga cgacgatgcg
    16921 cacgcgcttg ccgaacgcgc gcgctggttc gaagcgcaga ccgggcgcct gccgcgtatc
    16981 tccgtgatcg aatacaacgc gatcggcgac ggagatgtat tcacgcgatc accacgcgtc
    17041 gtcgaatatc gcaaagcact cgggttgccg agccatcgtc gctactccgg cggcagcgac
    17101 gtcgctgcag cgtgcggcca gctcgcgctg cggacctagc gctcgccgcg atcgaaaggt
    17161 ccggcttcgc tggaccgcgc agcatcgcga agcgatgcgg agtcgcgatc gtaggcgtcg
    17221 cgcgatgtct ggatgatctc gaacgctttg tcgtggccgt agaaccgatc gacctcgacc
    17281 atcttctctt cgagcgtctt gtagtcgcga aagaagcggt cgagctcttt catgacgtgc
    17341 ggcggcagct gctcgagcga ctgatactgc tcgtacgccg gatcatcgac gcacaccgcg
    17401 acgatcttat cgtcgccgcc tttgtcatcg accatgcgta gaccgccgag cgcgcgtgcg
    17461 cggacgatcg tgagcggcgc gacgggctct tgcatcagca cgaggatgtc gagcggatcg
    17521 ccatcgtcgg cgtgggtgcg cgggatgaag ccgtagttcg ccggatagtg aacggcgctg
    17581 tagagcacgc gatcgaggcg gagcaggccc gtcggtttgt cgacctcgta cttgagatgc
    17641 gagccgcgcg gaatctcgat caccgacggg acccacgtgc ggatgtcgct cgggacctcg
    17701 atgtcgtgga cgggatgcgc catgcgtgcc tgccttaggc cttcatcgac gggcgctcgc
    17761 tcacgcgctt gtgccacgcc gcgaagttgg gatgcgtctc ggcgttgatg cggacgttcg
    17821 acactttgcc gaagtcgagc gcgcacaacg ccgtgatgtc cgcgacggtg aagcgatcgc
    17881 ccgccatgta ttgccgccgg ctcagctcac cttcgagcca gtcgaagcgg agtgcgagct
    17941 gctcgcgcat gatctcggcg aagtccggcg cctgcttgat gcggccgacc cagaactgat
    18001 gcgtgttgcg gaagacttgc gagatcggaa ccagcagctc gagctcggcg tgacgattcc
    18061 acatctcgac ttgtgcgcgc tcccacgaat tcgcgccgaa caggttcggc tcgggatgct
    18121 cctcttcgac gaaccggcag atcgccatcg actcgcggag gatccgcccg tcctgcagct
    18181 cgagaaccgg caacagtgac agtggattct tgcggcggaa gtcgtcggtc tgatgcgccc
    18241 gatcggcgat gagcacctcg atcgtgtcgt acgccacgtt cttctcggcc aggaagatgc
    18301 gtacccgcct cgggttgggc gcgcgggggt cgtggtacag cagcatgcgg cgactgtacg
    18361 gccaaaaacc ggcagccgct ccgcggatct cgtacaataa aggcgtatgc gcatcgcggg
    18421 gttgctgctc ctcgtctgtc ttgctggcgg ttgtaagaac aagggtcaag acgccgacgc
    18481 ggcgccggat cccgctgctc tcaaagcgca gcaagagctg atcgcgaggc gcgacaagct
    18541 gctcgatgct cgccagaagc tccagcaaga ccgcgacaag atcgacgccg agatcaaaga
    18601 cattcaagcg aagggtggcg acgcgagcga gcagctgaag aagcgcgaag agctcgacac
    18661 ccagatcgag acgtcgacga gcgacctcat cggcatggtc aacagcaagc tcgactcgct
    18721 cagcaaagac aagagcgccc aggtcgccgc gcgcgaatcg gatctggcgt cacgcgagaa
    18781 gacgatcgcg gaacgcgaag cgcgcattgc cgagcgcgag cggttgctcg ctcaacgcga
    18841 tgccgaatcc gccgcgcgct ggaaagacac gtgctcgaca ggcgcagcgc ccgtcatcat
    18901 ccagcagacc gcgcccaagg gcggcaactg gacgaacaag gacgtgaccg cgctgttgca
    18961 gaaggcgcgc aacgcgatga acaagaaggg cctcatcaac tccgacttgc caggcccggc
    19021 cgcgagcttc gagagcgaag cgacgaaggc gatgagcgac aacgattcga accgcgcgta
    19081 catcgcggcc gcaaacctcg tcggcacgat cgacacgatc gtggtcaacc gtcagttcat
    19141 ccagcagaag acggcgcgca tccagagcca agcgaaagcg atcaagctcg acgaggcgac
    19201 gaacaaggat ctgcaggaca tcatcggcga cgtgatgaac agctacaaca acggcgactt
    19261 tgcggccgcg aacaagcgtc tgaatcaggc cgccgcgaag atcgcgaagc agtagcgagc
    19321 tagcagttct cgccgccgac tttgcggcag ttgtcgaact gcgggaagtc gtgcgcgacg
    19381 acgccggcga cgatgccgac gccgacggcg agcacgaaga tcgcgggacc cgcgacgtac
    19441 cagcgtttcc acaccggagg atccgtatag atgatcgtgt cgctcgcgcc gggcttggcg
    19501 tggatgccgc cctcgacgat cgggtactgc ttcatgccga cttgcacatc ggtgacggtg
    19561 ccgtacttga cgtccacgaa cttcacgaag ttgtgatact gcggatgcgt cacggtgacg
    19621 gcgtgcgttc cgacggggag cgcgatgtcc ttggccgccg agaggttcac cttcgtgccg
    19681 ttgacggcga cgcttgcgcc ggtgacgtcg aagttgaagc gcacgttgcc gcggtacttg
    19741 tcggggtcga ggaggcgcac cgctgcgccg gctggcgtgt ccgctgcgtc cttgctgccg
    19801 atcgcgagtg tcgtcgagcg aagctcttgg cctttgctca cgtcggtcgt cttcagatag
    19861 atgacgcgcg aatcgccgag gccgccgacc tcgccggtca cgacttgctc cgcgccgacg
    19921 agcttgccga cttctgcgat gcattgcgtg tcgtcttcgc actgcttgag ctgcggcttc
    19981 ttcgccttgt cgatcgccgc ggagacctgc gcggccggaa cgatcttcgt gccgggtagg
    20041 gcggctatcg cttgctcgat ctgggcgagc agcttcttcg tcgctgcgga tttgtcctcg
    20101 gcgccgagtg tcgagagcgg tgcgatcgca acgacgcggt cggcgtacgc gcgggcagcg
    20161 gtcgcagcga cgagcacgat gacgagcggg gttagtcgca cggcatgctc cgatcgccat
    20221 tgatgtagca gggcacgctg ccgatgttgc gtccggcgag aatgccgacg ccgacggcga
    20281 cgactgcgac ggtcgcgatg aaccacgtct tcgtgtagat cggcgtgcgc tccttgtaga
    20341 tcgtgcggcc ggtgccgacg gtctcgccgc tctggagcag gcgaacgacg acggggctca
    20401 ccttctggaa gtggacgtcg acgtcgctct cgaacgggtc gtagcccttc gccttcactt
    20461 cgagcttgtg cttaccgagc ttcagcttgc cgaggacgcc gccgtttggc agtggtgtgg
    20521 cgccgacctt ggtgccgtcg agcgcgacct ccgcaccgac gagatccgtc tggatctgca
    20581 gagcgccgtg cagctgctgg ggcgcgagca gacgataggc agcgacgcgc acgccctcga
    20641 tgagctcgtc gggagagccg cgcagcgggt cggattggat cttctgcgat ttgcccgtcg
    20701 cgacctcgac gaccttgatg ttgaggacgt agttgtcgcc gagcgagccg accgtgccgg
    20761 tgacgacgaa ctcgacgccg agcttcttgc cgatcgatac caggcacttc tcttcgcccg
    20821 tgcagttttg ctcggcggcg ctgacggtgc gctcgatgtc gcgacggctc ggttgcggaa
    20881 ccttgtcgag gcgatcgagc tccatgcgga acagcgtctc gaggcgctgc acgatctcgt
    20941 tctcgatgcc gagcgcgtcg aagcgccaga ccgcgatctt cttgtcgagc agatcctcgg
    21001 cggagggagc ctcctgcgcg tgcgccgcgg cgctagcgag cgcgatcaca gcgagcacga
    21061 acgaggcgac cctagccacg atggttcgat cctatcatta cctgcgggct gggcgcgctt
    21121 cgcggaggcc gtgatagcct gcaggcatgt catgggctcg ggtgttcgtg atcgcggcac
    21181 ttgtcacgtg cgctgccgca tgttcctcgg tgacgacgac gcgcgtgcag cccgagagcg
    21241 tcgcggtggg gccgcatatg cgcccgatcg cggtcgtcca cgcgcaggtc acgagcgcgt
    21301 acttcttgtt tctcgcgatc ccgggccacg tcagcctcga ctacgtggtg aatcgcatgt
    21361 tgattcacgc ggcgaaggag ctcggcgccg acaaggtcgt cgacatcaag gtcgatatca
    21421 cgcccggcga cggcatctgg acgttccgca agatcctcgg ctggcgctcg gcgcaggcca
    21481 gtggcatcgc ggtcgtcatg gaagatgagg cagcagcgcc cggtgccccg gccacggcga
    21541 cgcccgctca accgcagccg gccccaacca agtaggctat agatccctcg tgccgttgga
    21601 tgtcttcgac cgccgcctga tcctcgtcgt cgggaagggt ggtgtcggcc gcagcacggt
    21661 ggccgccgcg atcgcaggtc agtgcgcggc gcgtggtcgc aagacgctgc tgttcgagac
    21721 gaacgccaac gatcgcttcg gtacgtactt cgataagccg gccgtcggca ccgaggtcac
    21781 gcagctcgcg ccgaacttgc acgcggtgaa cacgaacccg gcacatgcgc tcgaagagta
    21841 cgggctcatg atcctgaagt tccgctcggt ctacgagatg gtgttcgaga accgtgtcac
    21901 ccgcgcgttc cttcgcgcga ttcccggtct cgacgactac gcgctgctcg gcaaagcgtg
    21961 gttccacacg acggaagaga agcgcggcaa gcctgtgtgg gacacggtcg tgttcgacat
    22021 gccggcatcg ggtcactcgg tcacgatgct gcggattccg tgggtcatca cggataccgt
    22081 ccccgaaggt ccgctcacgc gcgacgcgcg gacgatcaaa gagctgctca ccgatccgaa
    22141 gcggaccgcg gcggtgctcg tgacgctcgc cgaagagatg ccggtcaacg aagcgatcga
    22201 gctcgaagca aagctctcgg gcttcggcat cgcgccgcag cacgtcgtct gcaaccaggt
    22261 ctatccagcg cacttcgcgc cgggcgcacc ggtcacgaag gtcgtcgata cgctcgctgc
    22321 ggatccgaat cttccgtcac cgctgcgcga agtcaccgag cacgcgacgc tgtctcgcga
    22381 tcgccgcaag ctcaacgagc attacctgac cgagctgcgc gcgcgcacga agacgcccgt
    22441 gcaggagctg ccgatgttgt tcacgcccgc gctcgacgct gcgcacgtca aagcgctcgg
    22501 cgaccggttg tagctactcg ccggtctggc gcatcgggaa cagcggctcg ccatggatct
    22561 cgaggcggta gacgctgttc tcggtctcga cgtagatcat cttgccgccg ggctcaccga
    22621 gcacgcgctt caccggcgtc gtgatcatgc ggtggccgtg cgggtcgcga aagatcacca
    22681 tgccgcgccc gatgtacggg tacgagatca ggtatcccgt ataaattcgt ccgctggccc
    22741 caccgggtct ctcggagcca agctcacgaa tcttgcgtgc cgtgacgctg accatggacg
    22801 cgttacctat agtctaagag atggtcgtgg actcgtcatc ctctttgcgc tgcgatgaaa
    22861 aagcccatgg ctgagccgac catcgacgcg ctgtacgagc aggtgcgcac cgccgtaccg
    22921 gtcgccgccg gggacgtccc agccgagttg ctcccggtag ggccagggat tcgcgtgctt
    22981 gcgctgcgca cgccgacact ccctcccgca gctcacacca acgtgtacgt ggtcggaccc
    23041 gacgcagggc ctcagctcgt ggtcgatccc gggtcgccgt acccggatca gcaggcgatt
    23101 ctcgattcgg cgctcgagcg cgaggccgat gcgggccggc cgattggcgg cgtgctcctc
    23161 acgcatcatc acggcgatca cgtcggtggc gcggcagcac tggcagcgcg gttcggcgtt
    23221 ccgatcgcgg cgcaccgccg gacggccgag cggctgcgtg gtcgcgtgga tgtcgcgcgc
    23281 gagctcggcg acaacgagat cctcgacgac atcacgtgcg tcttcacgcc aggccacgcc
    23341 gacggtcacc tcgtcttcga gaaagccggc gcgcagattg cgggcgacat ggtcgcaggc
    23401 atcggcacga tcttgatcga tccgagtgaa ggcgacatgg ccgactacct cgcatcactc
    23461 gcccgcatgc tcgagcgcgc accgtcgctg ctgttgccgg cgcacgggcc accaatcacc
    23521 gacgctgccg gcaagctccg cgagtacatc gcacaccgca cgatgcgcga ggatcgcgta
    23581 gttgctgcgc tgaccagccg cgccgatgcc tccgtcgacg agctcgtgcc cgacgtgtat
    23641 gccgacacac ctcgcattct ttggcctctc gcaggtcgct cgctgcgcgc gcacctcgac
    23701 aagctcgtaa aacaaggcag agcgcgagag gtggagcccg ggcgatggac ggcccggtaa
    23761 ccttcgctat agtgctgccg cggtgggatt gaccttcgat acgctggccg aagtcgcgct
    23821 ggcgcgtgac atgttgcggc gctggtatgg cctcgagctc ggcctctccg cggccgacgg
    23881 ctccgactac gaccgcacgt cgcacgagac ctgcgagagc gtacgcaagc atccaagcgg
    23941 tggctgcgca gccgcgctca aagaagcggc gagcgtgctc gcgggacgca aaggcgcagc
    24001 cgcggtcgtc caccgctgtc atgcgggact gtccgtcgtc gctgcgccgg tccgctcgac
    24061 atcggggctc accgcgatcg tctactcgac gggcgggcgc acgaacgcgg aagcaaccag
    24121 ctcgatcggc acgctgctct cgctcggcgc cgacgaggcc accgcagccg cggcgcgcgc
    24181 gcccgagctc agcgacgccg acatcgcgca cgtgaaggat ctcgtcgagg ctgccgcaca
    24241 agcggtcgag cgcgcactac cgacggtgat tccgccgacg acgttcacac atccgttcac
    24301 cgaaatggtc ggcgacgcgc ccgccattcg cgaagtcgtc aagctgctca ccaaggtcgt
    24361 gaagagcgag gcgacggtcc tcattcacgg cgagagcggc acgggcaaag agctcatcgc
    24421 gcgcgcgatc cactatcacg ggcctcgcgc gaagaagacg ttcgtcgtcc agaactgctc
    24481 ggcgttcaac gacaacctgc tcgagagcgc gctgttcggt cacgtgcgcg gcgcgttcac
    24541 cggcgcggtc aaagatcaaa agggcctctt cgaggtcgcc gacggcggca cgttcttcct
    24601 cgacgaaatc ggcgacatgt ccgcggcgct ccaagtaaag ctgctgcgcg tcctccaaga
    24661 gggcacgttc acgccggtcg gcggcacgaa gccgatcaaa gtcgacgtcc gcatcatcgc
    24721 tgcgtcgcac aaggatctgc aggcgatggt cgcccacaag cagttccgcg aagacctgtt
    24781 ctatcgcgtc aacgttctca agatcaccgt gccgccgctg cgcgatcgcg tcgccgatat
    24841 cccgcgcctc gctgcgtact tcgtgacgaa gcactggaag tttcctggcc ccgcgccgcg
    24901 tctctcgcag agcgcgctcg ctcgcctcgt cgcgtatccc tggccgggca acattcgcga
    24961 gctcgagaac gagatcgagc gcatgctcgt cctcgcggcc gatgcgcaag agctgactgc
    25021 ctccatggtg tcgcagcgcc taggcggtca gtccgtcggc acgccgcgca cgctgcgcga
    25081 tgcactcgcg atcgccgaag ccggcgcgat caccgaatcg ctcggccgtc actcgggcga
    25141 tcgcgacgcg gccgccgcgg acctcaacgt ctctcgcgcg tacctcgaag cgcgtgtcgc
    25201 cgctctcggt ctctgatgac gatgaccagc gccaagaatg caaaggcgcg ctgcccgacg
    25261 tgccgcggtg tctcgctcaa agaggccaac aaggtgttcc cgttctgcag caatcgctgc
    25321 cagctgatcg acctcggtcg ctggctcgac gaggactatc gcgttcccga agaatcggat
    25381 gcgagcggcg gcggtatcga aggcatcccg cgcaccgacg acgaacgctg acgttcgtga
    25441 cgaaatgtct cgcgcgttct gcgcgttgag aacgtgatga cgcctgctag cgtttcggac
    25501 atgcggccct ccgtcgacga gccattcgat ccgatcgcgc tcgccattct cctcggcctc
    25561 gcgaacacga tcgtgatcgc attcgggctc gcggtggcca tccccgaacg cggtgtcgca
    25621 ggcgcgatct tcatcttcgg tttcatcccg ggaatgatga tcggcgcgat cgaaggtggc
    25681 atcgcacgcc tcacggcgcg cacaccgccg tgggtgcggc tcatcctgct gacgccgatc
    25741 ccagtaggct tcgtcctgtt gatggcgacg tcgctccact tccgcgcttc ctccaccggc
    25801 gccgccatcc cgactctcgt cgcggtcctg atcctcgagc gccactcgcg caaaaggccc
    25861 ctcgttcctc cggcgcgcgt agcctaagct cgagggatgg tggagcaccg cgtcctcgat
    25921 cacctcggcc tcgccgctgc cgactacgac gcagcgatcc gccgctacat cccgcactac
    25981 gacgcgctca tcgcgacgat cgtcgagctg gtcgacggcg acgtcaccga cctaggcacg
    26041 gggacgggcg cgctcgccgc agccatcctt acgaagccgt cgacgcgcgt gcgcctcgtc
    26101 gatatcgatc cagcgatgct gtcgaccgcc gcctcgcgcg tggccccctt cggcgaccga
    26161 gcgacatgcg tccgcgcgtc cttcgacgaa ggtctcgcgc catgcgacgc agtcgtcgcg
    26221 tcgttcgcgc tgcatcacgt gccgacgctc gaagcaaagc gttcgctata cgcgcgcatt
    26281 cacgccgcgc tgcggcccgg cggcatcctc gcgatcggcg acgtcacgat ccacgacgac
    26341 ggccccgcca acacgacgat gatgaacgcg tgggccgcat ggatgagcga ccacggcatc
    26401 gcgcgcgccg acgcgaacgc tctcttcgcg aagtgggctg gcgaagaccg ctacttctcg
    26461 ctggctgatg agctcgccac tctcgccgcc gctggcttct cgcgccccga ctgtttctgg
    26521 aagcacgggc cgtctaccgt cttcggcgcg taccgctagt gcgacgtcgc ttcgtctgac
    26581 gacgtatggt cgtgcaccac gcgccagcct tcaggccgcc gctcgagcgc gacgctgaac
    26641 acgccgccgc cggcatttgg cgtgcctgtc agcttccacc gcccgagcac gatcgcgcca
    26701 tccgcaccga gtgactgcac gctcag
//
LOCUS       DATLBZ010000485         6867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2856563, whole genome shotgun sequence.
ACCESSION   DATLBZ010000485 DATLBZ010000000
VERSION     DATLBZ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2856563"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VMZ53_24635"
     CDS             <1..389
                     /locus_tag="VMZ53_24635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31721.1"
                     /translation="RMLLREALLNVISNAAEACGPTNGDVSVRVRSIAGPAASAAPII
                     EIVVADTGPGIPRAHLGRLFVPGFTTKETGSGVGLAIAERVVSAHHGRITVDSEEGKG
                     TTITITLPTDKHGLAVLPRWTSAERG"
     gene            408..1832
                     /locus_tag="VMZ53_24640"
     CDS             408..1832
                     /locus_tag="VMZ53_24640"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS31722.1"
                     /translation="MIGTIVCVDDQAEVRRLLGEVFRAKGGNVVSFDDGEDAIKWLDT
                     NEADLVILDLDLGAGKRTGIEICKAMRAKLPELPIIILTGHGTIDDAVAAVKAGAVDF
                     ITKDPYLEDKLEISVEKVERIIKHAIDRKRLEQENKQLRATNERLREAAGPRWQIVGD
                     SQPMRTVMAKIEKVAAVPRPVLVLGPRGTGKELVARAIHLLSPRASEPYITINCAAVA
                     ESLLESELFGHEEGAFTGATKQKEGKFELADGGTLFLDEIGNMSLEFQAKILRVLEYQ
                     RFERVAGSESVQVNVRVIAATNADLKQAMADGKFRPDLYDRLAFEVIHLPPLADRMAD
                     VPVLATYFLMRFRQEVAGINVREIAADAFDRLAQYDYPGNIRELKNVVERAVYMAQGT
                     VLTGADVDLALPPEAQQAPVPGMTFADDPKLPLNERVDAFEAWLCKDALERTRYKQKE
                     AAALLGLTYDQFRQRYRKYALGKE"
     gene            1840..4086
                     /locus_tag="VMZ53_24645"
     CDS             1840..4086
                     /locus_tag="VMZ53_24645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31723.1"
                     /translation="MSLRVLFGFAVLLAGCLKFGGVPCDDGRTCPDGTECVDELKLCV
                     KHDQLTACADANGGDRCTAGAIQGVCNGAPLGMPCTTCVCVEARCGDGVVTAPEQCDT
                     DALGDEPVCNQHGYYETTNVACSPTCEYDVKASCSRTCGDGVVEAAFGEVCDDTVAPL
                     EPCISFGYDIGFADCIAGGCSPNLRQCRRIGWSPVVRETVQFNQHSLAAVGGHVFVVG
                     QTADLHLHVWHHDGVSWKNVDTGIAMLPYSLWAASPTDLYVLGTDTNNTTGRIAHLSG
                     STWTVENVPQNIAGEVWGTSSSDVWIFGYASARHFDGTTWTTVDDAGFGYIQAVWASA
                     PNDYWIVGGTALPHVLYHFDGSTWQTRATPVSELGGVSGTGAQPIVYGYGVAIWSGSA
                     FVSLDPPVVRVHAAWALSPTDIYIVADNSSVYHYDGVRWTLLYTTSFNARAIGGYAPD
                     GIYLVEGSGTIRHYDGNAWRLAPPAQAVPQPYVSVTGTTNAVFAVRQDAVDIFAGTRV
                     TETTLTSVRAVGRAGNDVYAVGADLIARYTGTPAWTADTAPTGFFVAIAGPDEATVFA
                     ATTKQIYRRNGNGTWTQVTLQGDPLAPNAIIEDLWCLPGGTTCVAVGQHVIIMWNGST
                     WTAQYELPHYGHVWGAAANDIYAVGGQGLILHFDGTAWSPIAIAGETSSFDGVYGTAS
                     NDVFIVTDGEALYHFDGRRWSPVRPFNAQGSVSDLWVSASAIWTIGGFSLTSTPRALV
                     LERTFPWQ"
     gene            4117..4881
                     /locus_tag="VMZ53_24650"
     CDS             4117..4881
                     /locus_tag="VMZ53_24650"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HUS31724.1"
                     /translation="MGIFGRIKKGVSSKANAALDKAIDPEKELEMAILELEEGRKKAL
                     AELVSYKATAKTLDNDMEKYKTKAAEWERRAMLAVKAGDDDAAKLALKEKKNCEQEAA
                     KIERDKHEAASYAIQLNKSRKEFETKLQILKMRKGTLATQIASARSANGDIFGNDKGV
                     WDKFARAEERIDQEAIETEVDAAMRGEDAEAALFDSKLAAAASQTPGLLTDGDDPLAA
                     LKEKMAVAKAEKAAKQLAAGDKKPAADDKAEKSEKK"
     gene            4878..5291
                     /locus_tag="VMZ53_24655"
     CDS             4878..5291
                     /locus_tag="VMZ53_24655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31725.1"
                     /translation="MIVTTHINARETTAPDQDEPLCTRLRDLALVDLDALTVGLDGTE
                     GQALTSAYADDVADALREARTRLATLRKELAGADPLNVLDMSSRQRASDAGHAGAARI
                     TDRLVTQTEAARAFARLAVLTAPLVGKLFEADRRR"
     gene            5367..5957
                     /locus_tag="VMZ53_24660"
     CDS             5367..5957
                     /locus_tag="VMZ53_24660"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS31726.1"
                     /translation="MGDEDPDLDRARNGDREAFGRLVRRHQKRVYAAALHILGNHSDA
                     DDATQETFVKAFRGLPQFDGRADFFTWLYRIAVNTALNTLRGGKRGSALSQKSGAEAA
                     HVGGRPEALGQNAPDPAQKAQTTGEVARVLEAIAELSPSLRVTLVLATIEDLPHKQIA
                     EILEIPEGTVAWRVNEARRLLRQRLAAVEPMSAVRK"
     gene            6082..>6867
                     /locus_tag="VMZ53_24665"
     CDS             6082..>6867
                     /locus_tag="VMZ53_24665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31727.1"
                     /translation="MQHGTGTTSDVKNAAMIPPGLEPDTQPTTKQDAVVEARDRDEDS
                     GLHDIRNLAQSTKQRLSSRRITNSPPMKDDDILASSSGSFKNIALPQPAKMVSLPELA
                     DLPSKKEVLAAEKAAAKEAKRSVKASKAPAIADDASVSLSAVAKVDAAVPAAPEVASV
                     PARSAFSLPSQQHKRSKAPLFAVIGLGAAAAAGAVIYTQMGGEKSQSAPAVAQVDNTA
                     KVEAQAPAPAAIAAPEERKKTDEELKAEAALEQAKLDAAKAEAD"
BASE COUNT         1316 a         2205 c         2278 g         1068 t
ORIGIN      
        1 atcgcatgct gctgcgcgag gcgctgctca acgtgatcag caacgcggct gaagcgtgtg
       61 gcccgacgaa cggtgacgtc tccgtccgcg ttcgctcgat tgcaggaccc gccgcgagtg
      121 cggcgccgat catcgagatc gtcgtcgcgg ataccggccc gggcatcccg cgcgcgcacc
      181 tcggccgctt gttcgtaccg ggcttcacga cgaaggagac cggctcgggc gtcggccttg
      241 cgatcgcgga acgcgtcgtg tctgcgcatc acgggcgcat caccgtcgat tcggaagagg
      301 gcaagggcac gactatcacg atcacgctgc cgaccgacaa gcacggcctc gccgtcctac
      361 cgcggtggac gagcgcggaa cgtggatgag atgatggcgg cgatccgatg atcggcacga
      421 tcgtttgcgt cgacgaccag gcagaagtac gccggctcct cggcgaagtc tttcgcgcca
      481 agggtggcaa cgtcgtttcg ttcgacgacg gcgaggacgc catcaagtgg ctcgatacga
      541 acgaggcgga cctcgtgatc ctcgatttgg atctcggcgc gggtaagcgc acggggatcg
      601 agatctgcaa ggcgatgcgc gcgaagctgc ccgagcttcc gatcatcatc ctgacggggc
      661 acggcacgat cgacgacgcg gtggcggctg tgaaagccgg cgcggtcgac ttcatcacga
      721 aggatccgta tctcgaggac aagctcgaga tctccgtcga gaaggtcgaa cgcatcatca
      781 agcacgcgat cgatcgcaag cgcctcgagc aagagaacaa gcagctgcgc gccacgaacg
      841 agcgcctgcg tgaagccgct gggccacgct ggcagatcgt cggcgactcg cagccaatgc
      901 gcacggtgat ggcgaagatc gagaaggtcg ccgcggtgcc gcgtcccgtg ctcgtcctcg
      961 ggccgcgtgg cacgggcaag gagctcgtcg ctcgcgcgat ccacttgctc tcgccgcgcg
     1021 cgagcgagcc gtacatcacg atcaactgcg cggccgtcgc ggagagtctc ctcgagagcg
     1081 agctgttcgg tcatgaagag ggcgcgttca caggcgcgac gaagcagaag gaaggcaagt
     1141 tcgagctcgc cgacggcggc acgctgttcc tcgacgagat cgggaacatg tcgctcgagt
     1201 tccaagccaa gatcctgcgc gtcctcgagt accaacgctt cgagcgcgta gcgggtagcg
     1261 agtcggttca ggtcaacgtg cgcgtgatcg cagcgacgaa tgcggacctc aagcaggcga
     1321 tggccgacgg caagttccgc cccgatctct acgaccgcct cgcgttcgag gtcattcatt
     1381 tgccgccgct cgccgatcgc atggcagacg tgccggtgct cgcgacgtac ttcttgatgc
     1441 ggttccgcca ggaagtcgcg ggcatcaacg tgcgcgagat cgccgccgac gcattcgatc
     1501 gcctcgcgca gtacgactat ccaggcaaca ttcgcgagct gaagaacgtc gtcgagcgcg
     1561 cggtgtacat ggcgcaaggc acggtgctca cgggtgccga cgtcgatctc gcgctgcctc
     1621 ccgaagcgca gcaggcaccg gtgccgggca tgacgttcgc cgacgatccg aagctgccgc
     1681 tcaacgagcg cgtcgacgcc ttcgaagcat ggctatgcaa agacgcgctc gagcgcactc
     1741 gctataagca gaaggaagcc gcggcgctct tgggcctcac gtacgaccag ttccgccagc
     1801 gctaccggaa gtacgcgctc ggcaaggagt agccgcacga tgtcgctacg cgtcttgttc
     1861 ggcttcgccg ttctgctcgc cggatgcctg aagttcggcg gcgtcccgtg cgacgacggt
     1921 cgaacgtgcc ccgacggcac cgaatgtgtc gacgagctga agctctgcgt gaagcacgac
     1981 cagctcacgg cctgcgccga tgcgaacggc ggcgaccgct gcacagccgg agccatccag
     2041 ggtgtctgca acggcgcgcc gctcggaatg ccgtgcacga cgtgcgtgtg cgtcgaagcg
     2101 cgctgcggcg acggcgtcgt caccgcaccc gagcagtgcg acacggatgc gctcggcgac
     2161 gagccggtgt gcaaccaaca cggctactac gagacaacga acgtcgcgtg ctcgccgacc
     2221 tgcgagtacg acgtgaaggc ctcatgctcg cggacgtgcg gcgacggtgt cgtcgaggcg
     2281 gcgttcggcg aggtctgcga cgacaccgtg gcgccgctcg aaccgtgcat ctcgttcggc
     2341 tacgacatcg gctttgcgga ttgcatcgct ggcggttgct cgccgaatct gcggcaatgc
     2401 cggcgcatcg gttggtcgcc cgtcgtgcgc gaaacggtcc agttcaatca gcactcgctc
     2461 gccgcggtgg gcggccacgt gttcgtcgtc gggcagaccg cggatctcca tcttcacgtt
     2521 tggcaccacg acggcgtctc gtggaagaac gtcgataccg gcatcgccat gctgccgtac
     2581 tcgctctggg ctgcatcgcc gaccgatctg tacgtgctcg gaaccgatac gaacaacacc
     2641 acgggccgca tcgctcacct cagtggttcg acgtggaccg tcgagaacgt gccgcagaac
     2701 atcgccggcg aagtctgggg cacctcgagc agcgacgtct ggatcttcgg ctacgcgagc
     2761 gctcgccact tcgacgggac gacgtggacc accgtcgatg acgccgggtt cggctacatc
     2821 caggcggtat gggcgagcgc gccgaacgac tactggatcg tcggcggaac ggcacttccg
     2881 cacgtgctct atcacttcga tggcagcacg tggcagacgc gcgcgacgcc ggtgtccgag
     2941 ctcggaggcg tgagcggcac cggcgcgcaa ccgatcgtct acggctatgg cgtcgcgatc
     3001 tggagtggca gcgcgttcgt ctcgctcgat ccgccggtcg tgcgtgtgca tgcggcgtgg
     3061 gcactgtcac cgacggacat ctacatcgtc gccgacaaca gcagcgtcta tcactacgat
     3121 ggggtgcgct ggacgctgct ctacacgact tcgttcaacg ctcgcgcgat cggcggatac
     3181 gcaccagacg gcatctacct cgtcgaggga agcggcacga ttcgccacta cgacggcaac
     3241 gcgtggcgat tggcgccgcc agcgcaagcg gtgccgcaac cgtacgtgtc cgtcaccggg
     3301 accaccaacg cggtcttcgc ggtgcgccag gacgccgtcg acatctttgc gggaacgcgc
     3361 gtgacggaaa cgacgttgac gagcgtgcgt gccgtcgggc gcgcaggcaa cgatgtgtat
     3421 gcagtcggcg cggacctcat tgcacgctac acggggacgc ccgcatggac tgcggacaca
     3481 gcgccgaccg ggttcttcgt cgcgatcgcc gggcccgacg aggccactgt cttcgccgcg
     3541 acgacgaaac agatctatcg ccgcaacggc aatggaacgt ggacgcaagt gacgctgcaa
     3601 ggcgatccgc tcgcgccgaa cgcgatcatc gaggacctgt ggtgccttcc gggcggaacg
     3661 acctgcgtcg ccgtcggtca gcacgtcatc atcatgtgga atggctcgac gtggacggcc
     3721 cagtacgaac tgccgcacta cggccacgta tggggcgcag ccgccaacga catttatgca
     3781 gtcggtggtc agggcctcat tctccacttc gatggcactg cgtggtcacc aatcgcgatc
     3841 gcgggcgaaa ccagctcgtt cgacggcgtg tacggcacag cttccaatga cgtgttcatc
     3901 gtcaccgacg gcgaggcgct gtatcacttc gatggccgcc gctggtcgcc ggtccgtccg
     3961 ttcaacgcgc aagggtcggt gagtgatctg tgggtctcgg cgtcggcgat ctggacgatc
     4021 ggcggcttca gcttgacgtc gacgccacgc gccctcgtcc tcgaacgaac attcccctgg
     4081 caataggcct ccgcgagtcg gtgctaagct cgtcccatgg gcatcttcgg caggatcaag
     4141 aaaggcgtct cgagcaaggc aaatgctgcg ctcgacaagg ccatcgatcc cgagaaagag
     4201 ctcgagatgg cgattctcga gctggaagaa gggcgcaaga aagcgctggc cgagctcgtc
     4261 tcgtacaagg cgacggcgaa gacactcgac aacgacatgg agaagtacaa gacgaaggcg
     4321 gccgagtggg agaggcgcgc catgctcgcg gtgaaagccg gcgacgatga cgcggcgaag
     4381 ctcgcgctca aagagaagaa gaactgcgag caagaggccg cgaagatcga gcgcgataag
     4441 cacgaggctg cgagctacgc gatccagctc aacaagagcc gcaaagagtt cgagacgaag
     4501 ctgcagatcc tgaagatgcg caagggcacg ctcgcgacgc agatcgcgtc ggcccgctcg
     4561 gcgaatggcg acatcttcgg caacgacaaa ggcgtgtggg acaagttcgc gcgcgccgaa
     4621 gagcgcatcg atcaagaagc gatcgagacg gaagtcgatg cggcgatgcg tggtgaggac
     4681 gccgaggccg cgctgttcga cagcaagctc gccgctgcgg cgtcgcagac gcccggcctg
     4741 ctcaccgacg gcgacgatcc gctcgcagcg ctcaaggaga agatggccgt cgccaaagcg
     4801 gagaaggctg cgaagcagct cgcggcggga gacaagaagc ccgcagccga cgacaaagcc
     4861 gagaagtccg agaagaagtg atcgtcacga ctcacatcaa cgcgcgtgag accaccgcgc
     4921 cggatcaaga cgagccgctg tgcacgcggc ttcgcgacct cgcgctcgtc gatctggatg
     4981 cgctcaccgt cgggctcgat gggacagagg ggcaggcgct cacctccgcg tacgccgatg
     5041 acgtcgccga cgcgctgcga gaagcgcgga cccgactcgc caccctgcgc aaagagctgg
     5101 ccggtgccga tccgctgaac gtcctcgaca tgtcgtcgcg ccagagggct tcggatgcgg
     5161 ggcacgccgg tgcggcccgt atcacggacc ggctggtcac ccagaccgag gcggctcggg
     5221 catttgcgcg cctggccgtg ctgaccgccc ccctggtggg caagctcttc gaagcggatc
     5281 gccgcagatg acgttgacgc cagatcggac ccaaacccgg gcgatgtgct gttgtccaat
     5341 ccccggtcac cttatagtcg cctgcgatgg gtgacgagga ccccgacctg gatcgggctc
     5401 gcaacggtga ccgagaagct ttcggtcgcc tcgttcggcg ccatcaaaaa cgcgtgtacg
     5461 ccgctgcact gcatatcctc ggcaatcaca gtgatgccga tgacgcgacg caagagacgt
     5521 tcgtcaaagc gtttcgcggg ctgccgcaat tcgatggtcg cgccgacttc tttacgtggc
     5581 tctatcgcat cgcggtgaac accgcgctca acacgctgcg aggtggcaaa cgcggatcgg
     5641 cgctctcgca aaagagcggc gccgaggctg cacacgtggg tgggcgaccc gaggcgttgg
     5701 gacagaacgc gccggacccg gcgcaaaaag cgcaaacgac cggtgaggtc gcgcgcgtgc
     5761 tcgaagccat cgcggagctg tctccatcgc tgcgcgtgac gctcgtgctc gcgacgatcg
     5821 aagacctgcc gcacaaacag atcgccgaga tcctggagat tcccgaggga acggttgcat
     5881 ggcgcgtcaa cgaggcccgc aggttgctgc gtcaacgcct cgctgccgtc gagcctatgt
     5941 cagccgtccg taagtaacgc catgcctgac gatttggaca accttcttcg cgctgcgatg
     6001 aaaaccctgg acgagcaggt gccctcgggt tatttcgaag cccttccgag tcacacgctc
     6061 gcgcgtttgg agggcagtag catgcagcat ggaacgggga cgacgtccga cgtcaagaat
     6121 gccgcgatga ttccgccggg cctcgagccc gacacgcagc cgacgacgaa gcaagacgct
     6181 gtcgtcgaag ctcgtgatcg cgacgaagac tctggccttc acgacattcg caatctcgcg
     6241 cagtcgacga agcagcgtct ctcgagcagg cgcatcacga acagcccgcc gatgaaggat
     6301 gacgacatcc tcgcgtcgtc gtcgggcagc ttcaagaaca tcgcgctgcc tcaaccggcg
     6361 aagatggtct cgctccccga gctggccgat cttccgtcga agaaggaagt gctcgccgcc
     6421 gagaaggctg cagcaaaaga agcgaagcgc agcgtaaagg cgtcgaaggc gccggcgatt
     6481 gcggacgacg cgtcggtgtc gctctcggcc gttgcgaagg tcgatgcggc ggttccggcc
     6541 gcccccgaag tcgcgtcggt tcccgctcgc tccgcgttct cgctgccgtc gcagcagcac
     6601 aagcgcagca aggcgccact gtttgccgtg atcggtctcg gcgctgccgc cgcggccggc
     6661 gctgtcatct acacgcagat gggtggcgag aagtcgcagt cggctccggc cgtcgcgcag
     6721 gtcgacaaca cggcgaaggt cgaagcgcag gctccggctc ccgccgcgat cgccgctcct
     6781 gaagagcgca agaagaccga cgaagagctg aaggccgaag ccgcgctcga gcaagcgaag
     6841 ctcgatgccg cgaaggccga agcggac
//
LOCUS       DATLBZ010000486         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6269868, whole genome shotgun sequence.
ACCESSION   DATLBZ010000486 DATLBZ010000000
VERSION     DATLBZ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6269868"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VMZ53_24670"
     CDS             <1..570
                     /locus_tag="VMZ53_24670"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUS31728.1"
                     /translation="FPGDDADWDFGLGAGFYLDATQAPWSAAYKMETHVVDELRAAVE
                     SAFPIARDRRGITGHSMGGHGALTLALRHADVYRSVSAFAPVCAPMQVPWGQKAFRNY
                     LATDGADHDACALITAGKRFMTPPLVDQGLADKFLETQLRPDLLEAACSAAGIELALR
                     RHAGFDHSYYFIATVVEDHLRHHAAHLQG"
     gene            677..841
                     /locus_tag="VMZ53_24675"
     CDS             677..841
                     /locus_tag="VMZ53_24675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31729.1"
                     /translation="MPSDYPTFVIASVHYALHLEGGVLTGTAAGQYTYDTPTTGDSCS
                     YAWDVTATKQ"
     gene            912..1895
                     /locus_tag="VMZ53_24680"
     CDS             912..1895
                     /locus_tag="VMZ53_24680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS31730.1"
                     /translation="MTIHDPQYAVEASGLVKQFGQLRAVDGIDLRVARGHVFGVLGPN
                     GAGKTTMLKMLATLLPIDGGHASIFGIDVAKRPHEVRQLVGVTGQYASVDENLTAREN
                     LMLFGRLLGLSRKGAAQTGDELLAAFGLADAADRQIKKFSGGMRRRLDLAVSLISRPP
                     LIFLDEPTTGLDPRTRGQMWETIRRLVREGATVLLTTQYLDEADQLADRIAVIDRGKV
                     VAEGTPGELKAGVGNSTLHVALEDRARTAEAAALVARVLGEEPVLTPEAGGLNIALSD
                     ANRAADVLIALRQAKLGISSASVRQPSLDEVFLALTGHHGDDQAERTEEAA"
     gene            1895..2713
                     /locus_tag="VMZ53_24685"
     CDS             1895..2713
                     /locus_tag="VMZ53_24685"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS31731.1"
                     /translation="MATLLPANAARMRVLAARPTIREALDQSFAMAWRALLKMRRNPE
                     QFFDVTLQPLLFTAMFTYIFGGAVAGSTAGYLPIIIPGILAQTTLTACMATGIQLRED
                     MDKGVFDRFKSLPIARISPLVGSALADLLRYALAATLTIIVGIAMGYRPGGGVAGVLG
                     GWLLTIIAGWSLAWVFTWLGAIARSAQAVQGISMMIMFPLSFLSNAFVPTQTMPGWLQ
                     TFVNVNPVSLVIRAVRDLMNHGEVTSSVGWALFGCAATVSIFAPLAVRAYSRKM"
     gene            complement(2714..>3101)
                     /locus_tag="VMZ53_24690"
     CDS             complement(2714..>3101)
                     /locus_tag="VMZ53_24690"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31732.1"
                     /translation="PRRHYRTGDAGRVVDGELRFEGRLDGQIKLFGHRIELDDIAADL
                     TALPNVARGVVIAVEEGGRAVALVGFVVGAPDARLDPRQLRDELATRVPSYMVPREIR
                     VVDALPMTVNGKLDRRSLAELSRERP"
BASE COUNT          521 a         1029 c         1039 g          512 t
ORIGIN      
        1 tttcccggcg acgatgcaga ctgggacttc ggcctcggcg cgggctttta tctcgatgcg
       61 acgcaagcgc cgtggtccgc cgcgtacaag atggagacgc acgtcgtcga cgagctgcgt
      121 gctgccgtcg aaagcgcgtt cccgatcgcc cgcgaccggc gcggcatcac gggccactcg
      181 atgggagggc acggcgcgct gacgttggcg ctgcgccacg cggacgtcta tcgctcggtg
      241 tcggccttcg cgccggtgtg cgcgccgatg caggtgccat ggggccaaaa ggcatttcgc
      301 aactacctcg cgacggacgg cgcagaccac gacgcgtgcg cgctgatcac cgcgggcaaa
      361 cgcttcatga cgccgccgct cgtcgaccaa ggcctcgccg acaaattcct cgagacgcag
      421 ctccgccccg atctactcga agccgcatgc agcgcggcgg gaatcgagct ggcgctgcgg
      481 cggcacgcgg gcttcgatca cagctactac ttcatcgcga ctgtcgtcga agatcacctt
      541 cgccatcacg cggcgcactt gcaggggtga ctggaaggta gcatcgccgg tcgacagcga
      601 gccgtgcaag ccgctcacga cgaccggcac tgcgacggag ttcgatctct cggagcgctg
      661 gaatgccggc gccggcgtgc ccagcgatta tccgacgttc gtcatcgcgt cggtgcacta
      721 cgcgctgcat ctcgagggcg gcgtccttac cgggaccgcg gcaggccaat acacgtatga
      781 cacgccgacc acgggcgata gctgcagcta cgcgtgggac gtgaccgcga ccaagcagta
      841 gagcacgcaa ggtttcttca cgaagcgcca cgtcgttgcg caatgacgag gtgtttcgat
      901 gtgtgctcga tatgacaatc catgatccgc agtacgcggt cgaggccagc ggcctcgtga
      961 aacagttcgg ccagcttcgt gccgtcgacg gcatcgacct gcgagtggca cgcggccacg
     1021 tcttcggagt gctcggtccc aacggtgccg gcaagacgac catgctgaag atgttggcga
     1081 cgcttctgcc gatcgacggc gggcacgcga gcatcttcgg catcgatgtc gcgaagcggc
     1141 cgcacgaggt ccgccagctc gtcggtgtaa cggggcagta cgcctccgtc gacgagaacc
     1201 tgactgcgcg cgagaacctg atgctgttcg gacggctgct cggcttgtcg cgcaagggcg
     1261 cggcacagac cggtgacgag ctcctcgctg cgtttggcct cgccgatgcg gccgaccgcc
     1321 agatcaagaa attctccggc ggaatgcggc ggcgactcga cctcgcggtc tcgctgatca
     1381 gccggccacc gctgatcttc ctcgatgagc cgacgacggg cctcgatccg cgcacgcgcg
     1441 gccagatgtg ggaaacgatt cggcggctcg tgcgagaagg cgcgaccgtc ctgctcacga
     1501 cgcagtatct cgacgaagcc gatcagctcg ccgatcgcat tgcggtgatc gaccgcggca
     1561 aggtcgtcgc cgaagggacg ccgggtgagc tcaaggccgg cgtcggcaac tcgacgctcc
     1621 acgtggcgct cgaggatcgc gcacgcacgg cagaagcagc agccctcgtc gcgcgcgtgc
     1681 tcggcgagga acccgtgctg acgcccgaag ccggcggcct caatattgcg ctgtccgatg
     1741 cgaaccgcgc cgccgacgtc ctgatcgcgc tgcggcaagc gaagctcggc atctcgtcgg
     1801 caagcgtccg gcaaccaagc ctcgacgagg tgttcctcgc gctgacgggc caccacggcg
     1861 atgaccaagc ggagcgcacc gaggaggcgg cgtaatggcg accttgcttc cagccaacgc
     1921 ggctcgcatg cgcgtactcg ctgctcgccc gacgatccga gaagcgctcg atcagtcatt
     1981 cgcgatggcg tggcgcgcgc tgctcaagat gcgccgcaac ccggagcagt tcttcgacgt
     2041 gacgctgcag ccgctgctct tcaccgcgat gttcacgtac atcttcggcg gagcggttgc
     2101 gggcagcacg gctggctact tgccgatcat catcccgggc attctcgcgc agacgacact
     2161 cacggcgtgt atggcgactg gcatccaact ccgcgaggac atggacaagg gcgtattcga
     2221 tcgcttcaag tcgctgccga tcgcgcgcat ctcgccgctc gtcggctcgg cgctcgccga
     2281 tcttctccgt tacgcgcttg ccgcaacgct gacgatcatt gtcggcatcg cgatgggata
     2341 ccgccctggc ggcggcgtcg caggcgtgct cggcggctgg ctgctcacga tcatcgcagg
     2401 ctggtcgctc gcgtgggtct tcacgtggct cggcgcgatc gcacgcagcg cgcaggcagt
     2461 gcaaggcatc tcgatgatga tcatgttccc gctgtcgttc ctgtcgaacg cgttcgtgcc
     2521 gacgcagacg atgccgggtt ggctgcagac gttcgtcaac gtgaaccccg tgtcgctggt
     2581 gattcgcgcg gtgcgcgatc tcatgaatca cggcgaggtg acgagcagcg tcggttgggc
     2641 actgttcggc tgcgccgcga cggtctcgat cttcgcgccg ctcgcggttc gcgcatacag
     2701 ccgcaagatg tagctacggc cgctcacgag acagctcggc gagagagcgg cgatcgagct
     2761 tgccgttgac ggtcatcggc agtgcgtcga cgacgcggat ctcgcgcgga accatgtacg
     2821 agggaacgcg cgtggcaagc tcgtcgcgca gctgtcgcgg gtcgaggcgc gcgtccggcg
     2881 caccgacgac aaagccgacg agcgcgacgg cgcgaccgcc ttcctcgacg gcgatgacga
     2941 ctccgcgggc gacattcgga agcgcggtga gatcggcggc gatgtcgtcg agctcgatgc
     3001 gatggccgaa gagcttgatc tgcccatcga ggcggccttc gaaccgaagc tcgccgtcga
     3061 caactcgccc cgcatcaccg gtgcggtagt gacggcgcgg c
//
LOCUS       DATLBZ010000487         5016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_632254, whole genome shotgun sequence.
ACCESSION   DATLBZ010000487 DATLBZ010000000
VERSION     DATLBZ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632254"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..517
                     /locus_tag="VMZ53_24695"
     CDS             272..517
                     /locus_tag="VMZ53_24695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31733.1"
                     /translation="MRARSIEHDIVLLPRWDADLNEKGERAELASTLLFAAGGAAVVG
                     GVVMIVLRYRAPEDATKTSLHVVPRADGASVWWSGAF"
     gene            complement(524..1483)
                     /locus_tag="VMZ53_24700"
     CDS             complement(524..1483)
                     /locus_tag="VMZ53_24700"
                     /inference="COORDINATES: protein motif:HMM:NF015657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31734.1"
                     /translation="MRTYTLAALLLVVGCGKDPALGGGDDMPPTDADTGSGSADAYIP
                     PNGYTKLIGRTWSTPAQGKDIYKCIRITVPTEMYITDIIAQAPLGTHHTVLSIASGNA
                     AGADGEQDCDVGTIGRQMLYASGVGTDPFSFPAGVGLKVAANTQLHLNLHLYNASDDP
                     MSDESAIWVKSQTTPPAMLAEMVFAGKILFGVGTGTQTITGGCTVAQNQGFNLFAVWP
                     HMHKLATHSKFEIIRGTNPPNVLWDAAYDFNDQKYYAPNGSPEFQVQAGDQIKVTCSY
                     NNTTGHSVTFGDSSDNEMCFVGMYRYPSLNASLTQCTDRPSGF"
     gene            complement(1575..2804)
                     /locus_tag="VMZ53_24705"
     CDS             complement(1575..2804)
                     /locus_tag="VMZ53_24705"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HUS31735.1"
                     /translation="MTAPDGQGKPDEAAVGFVMALGRALHRYGTPAHRLEDSLVKVCA
                     GLGIAAEVFTSPTALIMSFGRPTDLRSRMLRVDASVLDMGKLAEVDGVADDVIAHEIT
                     PEEGERRLEQIIAGKRRYGHGLSTLVHGVTAGALAVFFHGGLYDVAAAAGIGLTLGLL
                     AQLLQRSTDQARVFELVGSAFAAFAANVFSSVTPHITASLVTLAALVVLLPGMSLTVA
                     MTELATRHLIAGTARLMSAVIVLLELVVGVALGDRAAQALVHVHQATPVALPEYANWI
                     ALAASSVALAVLVQAQVRAFGWIVAGCVVAYVGSRFGAVWLGDNQLGVLIGAFALGLL
                     ANIYARYLDRPSQVVSVPAMLVLVPGGMGFRGMASLLNQDTLTGVETLFAMFIVAIAI
                     VAGLLIANALVSPRRSL"
     gene            3106..4836
                     /locus_tag="VMZ53_24710"
     CDS             3106..4836
                     /locus_tag="VMZ53_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HUS31736.1"
                     /translation="MQNMLVVNSEGPETRVAVVEEGSLAEFFVERKRDRGMVGNIYRG
                     KVTRVLPGMQAAFVDLGSKVERAAFLYVADVLGAGEERKLFEDSETDDGGDESPEGAA
                     SRIARSRKQLANRKIEDLLKPGQTVVCQVVKDPIGLKGARVTGYISLPGRYSVFMPHV
                     AQVGVSKRIGSDKERRRLRDLVNEARPKGTGFIVRTAAEDANDQELRDDVDFLARLWG
                     EIEKREESMNGPGLVYADLDLTLRCIRDLMREDTSEIIIDDEEQYDRVRKFTMAFLPR
                     FTERVKKYEQRRPIFDHYAIEPALRLAVSRRVPLRSGGSLVIDQGEALTAIDINTGSF
                     TNTGSGNLEDTVTANNLEACEEVARQLRLRNIGGIIVVDFVDMDKEGNRRKVWDAFQK
                     ALSKDRARTNVTKISELGLVEMTRKRTRESLVQLLTEPCPTCEGSGVVKSVTTVAYEI
                     LREVRRSGMLVDNEKIEIEAAPRVAEVLQRQERDYLDSLEKRFQKLIIVVGQKGWKPD
                     QFRVAGKMSTEIAAQAAAEAPHAHIPHAARNGGGGGGGQQGGGGGAGGGGRRRRRRGG
                     RGRDRANADA"
BASE COUNT          939 a         1534 c         1705 g          838 t
ORIGIN      
        1 gtgcgcgaag cggacagcat cgacgacatc accgacagac tcatcatcga cgggaacaac
       61 gacgactgca gcgacgactg gaacgacgac tgcagcgacg actggaacaa cgactgcagc
      121 gacgactgaa acgacgactg cagcgacgac tgaaacgacg ccagcgcctt catcgccgcg
      181 cgacgacgtt tctcacggct cgctatcgat cgccggactg gcggtcgccg ggacaggcgt
      241 cgcgtgtatc gcgggcggcg tcctgttcgg cttgcgcgca cgctccatcg agcacgacat
      301 cgtactgttg cctcgctggg atgcagatct caacgagaag ggcgagcgag cggagcttgc
      361 atcgacgctg ttgttcgcgg caggcggtgc ggccgtcgtc ggcggcgtcg tgatgatcgt
      421 cctgcgctat cgcgcgcccg aggatgctac gaagacgagc ctgcacgtcg tcccgcgtgc
      481 ggacggcgcg agcgtttggt ggtcgggcgc cttctgatcg tcgctagaaa ccgctcggac
      541 ggtccgtgca ctgcgtcagg ctcgcgttca gcgacggata gcgatacatg ccgacgaagc
      601 acatctcgtt gtccgagctg tcgccgaatg tcacgctgtg gcccgtcgtg ttgttgtagc
      661 tgcaggtgac cttgatctga tcgccggcct gcacctggaa ctcgggcgag ccgttcggcg
      721 cgtaatactt ctggtcgttg aagtcgtatg ccgcgtccca cagcacgttc ggcgggttcg
      781 tgccgcggat gatctcgaac ttcgaatgag tggccagctt gtgcatgtgc ggccacaccg
      841 cgaagaggtt gaagccttgg ttttgggcca ccgtgcagcc accggtgatc gtttgcgtgc
      901 cggtgccgac gccgaacagg atctttccgg cgaacaccat ctccgcgagc atcgccggcg
      961 gcgtcgtttg cgacttcacc cagatcgctg actcgtcgct catcggatcg tcgctcgcgt
     1021 tgtagaggtg caggttcaag tgcagctgcg tgttcgctgc gaccttgagg ccgacgcccg
     1081 ccgggaacga gaatggatcg gtgcccacgc ccgacgcgta gagcatctga cggccgatcg
     1141 ttccgacgtc gcagtcttgc tcgccgtcgg cgccggcggc gttgccgctc gcgatcgaga
     1201 ggacggtgtg atgcgtgccg agcggcgcct gcgcgatgat gtcggtgatg tacatctcgg
     1261 tcggcaccgt gatgcgaatg cacttgtaga tgtctttgcc ttgcgcgggc gtggaccacg
     1321 tgcggccgat gagcttcgtg tagccgttcg gtgggatgta tgcatcggcg cttccgctgc
     1381 cggtgtccgc gtcggtgggc ggcatgtcgt cgcctccacc aagagcgggg tccttgccac
     1441 atccgaccac gagcaaaagc gcggctagcg tgtacgtcct catcgagggc atcatcctcg
     1501 atctcagacg cgtgcaacgg gaaaagtgtt gctggcgata tgcagccgat cacagacggc
     1561 gacgcggcgg cgcctcataa gctgcggcgc ggagacacca gcgcgttggc aatgaggagt
     1621 cccgcgacga tagcgatcgc gacgatgaac atggcgaaca gggtctcgac gccggtgagg
     1681 gtgtcctggt tcaggagcga cgccatcccg cggaagccca tgccgccggg gacgaggacg
     1741 agcatcgccg ggaccgacac cacctgcgag ggccgatcga ggtagcgagc gtagatgttg
     1801 gcgaggaggc cgagcgcgaa cgcgccgatc aggacgccga gctggttgtc gccgagccag
     1861 acagcgccga agcggctgcc gacgtacgcc acgacgcagc cggcgacgat ccagccgaac
     1921 gcgcggacct gcgcctgcac gagaacagcg agagcgactg acgacgcagc gagcgcgatc
     1981 cagttcgcgt actcgggcag ggcgaccggt gttgcttgat ggacgtggac gagggcctgc
     2041 gccgcgcgat cgccgagcgc gacaccgacg acgagctcga gcaagacgat cacggcggac
     2101 atgagacgcg cggtgccggc gatcagatgt cgcgtcgcaa gctcggtcat cgcgacggtt
     2161 agcgacatgc ccggtaagag gacgaccagt gctgcgagcg tgacgagcga cgccgtgatg
     2221 tgcggtgtta ccgacgagaa aacgttggcg gcgaacgcag cgaacgcgga gcccacgagc
     2281 tcgaacacac gcgcttgatc agtcgagcgt tggagaagct gcgcgaggag gccgagcgtg
     2341 agcccgatgc cggcagccgc ggcgacgtcg tagaggccgc catggaagaa gaccgcgagt
     2401 gcgccggctg tcacgccgtg cacgagggtc gacagcccgt ggccgtagcg gcgcttgccc
     2461 gcgatgatct gttcgagacg gcgctcgcct tcttcgggcg tgatctcgtg cgcgatgaca
     2521 tcgtccgcga cgccatcgac ttcggcgagc ttgcccatat cgaggacgct cgcatcgacg
     2581 cgcagcatcc ggctccggag gtcggtcggc cggccaaagc tcatgatgag tgcggtcggc
     2641 gaggtgaaga cctcggccgc gatgccgagc cccgcgcaga ccttcaccag gctgtcctcg
     2701 aggcggtgtg cgggcgttcc gtagcgatgg agggcacgcc cgagcgccat cacgaagcca
     2761 accgcagcct catcgggctt gccttggcca tccggagcgg tcacgtcgcc aggttagcgg
     2821 aagtgcccga aaagcacgaa ggtccgggcg gcaacccaga ccttcgactt acttaaataa
     2881 ctagagaaag actaagttag gccgatgcgg caacaccggc gtgcccgtgt caaagcaagg
     2941 ggtaggccag taggctgggc ccggctgtaa tgggttgttt tggttggagt gccgtgctac
     3001 cgaggcctct caaaccaagt ttccgcgagc ccgacttgcg acatggatgt cgcgactgtc
     3061 cggagtgctc gcaacagatt tcaatacgga tagagcgtct ttcacatgca gaacatgctc
     3121 gtggtcaatt cagagggacc cgagacccgc gtcgcggtcg tcgaagaggg atccctcgct
     3181 gagttttttg tcgagcgcaa acgcgatcga ggaatggtcg gaaacatcta ccgcggaaaa
     3241 gtcacgcggg tgcttccggg tatgcaggca gccttcgtcg atctcggatc gaaggtcgaa
     3301 cgcgccgcat tcctctacgt cgccgatgtg ctcggtgcag gtgaagagcg aaagctcttc
     3361 gaggacagcg agaccgatga tggaggagac gagtcgccgg aaggcgcagc gtcccgcatc
     3421 gcgcggtcgc gcaagcagct cgcgaatcgc aagatcgaag atctgttgaa gccaggacag
     3481 acggtcgtct gtcaggtcgt caaagatccg atcgggctca agggcgcgcg cgtcaccggc
     3541 tacatctcgt tgcctggtcg ctactccgtc ttcatgccgc acgtcgcgca ggttggcgtc
     3601 agcaagcgca tcggctccga caaggagcgc aggcggcttc gcgatctcgt caatgaagcg
     3661 cggccgaagg gcacaggctt catcgtgcgc accgcggccg aggatgcgaa cgatcaggag
     3721 cttcgcgacg acgtcgactt cctcgcacgc ctgtggggcg agatcgagaa gcgcgaagag
     3781 tcgatgaacg gtcccggcct cgtgtacgcc gatctcgatc tcacgcttcg ctgcattcgc
     3841 gatctcatgc gcgaagacac gagcgagatc atcatcgacg acgaagagca gtacgaccgc
     3901 gtccgcaagt tcacgatggc attcctcccg cgcttcaccg agcgcgtgaa gaagtacgag
     3961 cagcgtcgtc cgatcttcga tcactatgcg atcgagcctg cactgcgcct cgccgtttcg
     4021 cgtcgcgttc cgctgcgcag cggtggctcg ctcgtgatcg atcagggcga agcgctcacc
     4081 gccatcgata tcaacaccgg cagcttcacg aacaccggct cgggcaatct cgaggacacg
     4141 gtcaccgcga acaacctcga agcgtgtgaa gaggtcgcgc gccagctccg gctgcgcaac
     4201 atcggcggca tcatcgtcgt cgacttcgtc gacatggaca aagagggcaa ccgccgcaag
     4261 gtgtgggacg ccttccagaa ggcgctgtcg aaggatcgcg cgcgtacgaa cgtcacgaag
     4321 atcagcgagc tcggcctcgt cgaaatgacg cgcaagcgca cgcgcgagtc gctcgtccag
     4381 ctgctcaccg agccgtgccc gacgtgcgaa ggctctggcg tcgtgaagag cgtgacgacc
     4441 gtcgcgtacg agatcctgcg cgaggttcgt cgctccggca tgctcgtcga caacgagaag
     4501 atcgagatcg aagccgcgcc gcgcgtcgcc gaggtgctgc agcgccaaga gcgcgactat
     4561 ctcgactcgc tcgagaagcg cttccagaag ctcatcatcg tcgtcggtca gaagggctgg
     4621 aagccggatc agttccgcgt cgccggcaag atgtcgaccg agatcgccgc gcaggcagcc
     4681 gctgaagcgc cgcacgctca catcccgcac gcagctcgca acggcggtgg tggtggtggc
     4741 ggccagcagg gtggcggcgg cggtgcaggt ggaggtggga gacggcgtcg ccgtcgcggt
     4801 ggccgtggtc gcgatcgagc gaacgcagac gcctaggtgt tgtgtggcgg cgctgcgtca
     4861 gtcgtgaacg acgcgcgcta ccctcgacga gatcacgcga gttagccaca gactggaagc
     4921 acggcaccat tcgtgcgttg atggtctgaa catgcgtggc ttagttgcag tggcacttct
     4981 cgcgggctgt ggcggtgcgt ccgccagtgg cagcgc
//
LOCUS       DATLBZ010000488         2822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_634688, whole genome shotgun sequence.
ACCESSION   DATLBZ010000488 DATLBZ010000000
VERSION     DATLBZ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634688"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VMZ53_24715"
     CDS             <1..273
                     /locus_tag="VMZ53_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HUS31737.1"
                     /translation="KNALAKQYHRLFELEGVKLNFRPDALKAIAEEASRRNSGARGLR
                     AILEEVMLDVMYEIPSLTGVKECVVTREVVEHRARPELVLESKAAS"
     gene            complement(354..1292)
                     /locus_tag="VMZ53_24720"
     CDS             complement(354..1292)
                     /locus_tag="VMZ53_24720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31738.1"
                     /translation="MRAGWAVSVVALAACGGPHSGSPDADANGSDADGMVDGGDGWKM
                     LIQRPWTVGSASEQFRCRRQTITEDMYITGLRALAPTGTHHTLLTIPDVQGTNGNFDC
                     TPNEISGTPQLLFGGGIGTNDFLFPPGVAVKIPAGTQLQLYLHIYNASDATMNDTSGI
                     LVKTVPASEVVNEADMFFVGKRTFQIPPDATPADPPAPYSITTDCGNGTLGDWHVVGM
                     WPHMHQHASHQKVEITRANVTMKTPLDVAFDFKEQRNYAMDYVVPPNDRIAVTCTWLN
                     DTGSMLYSGDNFGNEMCYAGLFVYPKVGNLYGCVNN"
     gene            1406..1843
                     /locus_tag="VMZ53_24725"
     CDS             1406..1843
                     /locus_tag="VMZ53_24725"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HUS31739.1"
                     /translation="MPELRNVIVFAIGAARYAVELRWVREVVTLGFVTAVPTAPPALG
                     GVCNLHGTILPVLDVSALLDQPPGAPARQGDGALVIEADGMICGLRVDQVDHVATLPE
                     RSGESGAVVDAGGRPLTLLDPQRLLRRAVELVNAAIEPAAVSG"
     gene            1840..>2822
                     /locus_tag="VMZ53_24730"
     CDS             1840..>2822
                     /locus_tag="VMZ53_24730"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HUS31740.1"
                     /translation="MSETDLDFDPEELAMLRQLFRTEAHDALEAVTSRVLAGGSVKPS
                     SDAFTEMMRVTHTLKGAAGTVGLDVMVDLTHRLESAFAALNRDATQWLPSTADLIVEV
                     TDGLRGYLDELANDPISADKHAMRLREQIERIARAEGSTPGTAISQIRESIAPPLRDS
                     MQDLLAPSPNDSLSMPTMTVLADDVLVESALEAPSTDVVPEPKSYLRVEPERVDALMS
                     SAGELLFDRTRIERRVQLLRTLARDLARTRQDLRDAIARDAPGKTSLGDAEAELASQA
                     ALLSQTTAALLDEIEALRRTIGDLQKGLTRIRMDSARNLFLHAARTLRALRR"
BASE COUNT          450 a          918 c          951 g          503 t
ORIGIN      
        1 aagaacgcgc tcgcgaagca gtatcaccgc ctgttcgagc tcgaaggcgt gaagctcaac
       61 ttccgccccg acgcgctcaa ggcgatcgcc gaggaagcct cgcgccgcaa ctccggcgct
      121 cgcggcctgc gcgcgattct cgaagaggtg atgctcgacg tgatgtacga gattccgtcg
      181 ctcacgggcg tcaaggaatg cgtcgtcacg cgcgaggttg tcgagcaccg cgcccggccc
      241 gagctcgtgc tcgagtcgaa ggccgcgagc tagctcagcg cgctcgtcgc attcgcagcg
      301 cgctcgtcgc attcgcagca cgctcgtcgc gttctcaacg cgttagcagc gcgctagttg
      361 ttcacgcagc cgtaaaggtt gccgaccttc ggatatacga acaggcccgc gtaacacatc
      421 tcgttgccga agttgtcgcc cgagtagagc atcgagcccg tgtcgttgag ccacgtgcac
      481 gtcacggcga tgcgatcgtt cggcggcacg acgtagtcca tcgcgtagtt gcgttgctcc
      541 ttgaagtcga acgcgacgtc gagcggcgtc ttcatcgtga cgttggcgcg cgtgatctcg
      601 accttctgat gcgacgcatg ctggtgcatg tgcggccaca ttccgacgac gtgccagtcg
      661 ccgagcgtac cgttgccgca atcggtcgtg atgctgtaag gcgcgggcgg gtcagccggc
      721 gtcgcgtccg gcggaatctg aaacgtccgt ttgccgacga agaacatgtc ggcctcgttc
      781 acgacctcgc tcgcgggcac ggtcttcacg aggatgccgg acgtatcgtt catcgtcgcg
      841 tccgacgcgt tgtagatgtg caggtagagc tgcagctgcg tgcctgcggg aatcttcacc
      901 gcgacgccgg gcgggaacag gaaatcattc gtgccgatgc cgccgccgaa caggagctgc
      961 ggcgtgccgg agatttcgtt cggcgtgcag tcaaagtttc cgttcgtgcc ttgaacgtcg
     1021 ggaatcgtca gcagcgtgtg gtgcgtgccg gtcggcgcga gcgcgcgcag gccggtgatg
     1081 tacatgtctt cggtgatcgt ctggcggcgg cagcggaact gctcgctcgc tgagcccacg
     1141 gtccacggcc gctggatgag catcttccag ccatcgccgc cgtcgaccat cccgtctgca
     1201 tcactgccgt tcgcatcggc gtccggcgat ccgctgtggg gaccaccgca cgcggcgaga
     1261 gcgacaacgc tgaccgccca accggctcgc atggtcgcag tatagagcta cctggggcgc
     1321 tggctggcat tttctccgcc gcaggtgtga ggcgcgcgag gattcgggag gccccgcgtg
     1381 ccggtggtca cggtatgctg ccgccgtgcc agagctgcgc aacgtcatcg tatttgcgat
     1441 cggtgcggca cgttacgcag tcgagcttcg ttgggttcgt gaagtcgtga cgctcggctt
     1501 tgtcactgcg gttccgacag caccgcccgc actcggtggt gtctgcaatt tgcacggaac
     1561 gatcttgccg gttttggatg tcagcgcatt gctcgatcag ccgccgggcg cacccgcgag
     1621 gcagggagat ggcgcactgg tcatcgaagc agacggcatg atctgcgggc tgcgcgtcga
     1681 tcaggtcgac cacgttgcga cgttgcccga gcgcagcggt gagagcggcg cggtcgtcga
     1741 cgctggtggt cgcccgctga cgctgctcga tccgcagcgc ttgcttcggc gcgccgtcga
     1801 gctcgtgaat gcggcgatcg agcccgcagc ggtttcggga tgagcgagac ggatctcgac
     1861 ttcgatcccg aagagctggc gatgctgcgc cagttgtttc gcaccgaggc gcacgacgcc
     1921 ctcgaagcgg tgacgtcgcg cgtgctcgcc ggcggatccg tgaagccgtc gagcgatgcg
     1981 ttcaccgaga tgatgcgcgt cacgcatacg ctcaagggcg cggcgggcac cgtgggcctc
     2041 gacgtgatgg tcgacctgac gcaccggctc gagagcgcgt ttgcagcgct caaccgcgac
     2101 gcgacgcagt ggttgccgtc gacggccgac ctcatcgtcg aggtcacaga cggcctgcgc
     2161 ggctacctcg acgagctcgc gaacgatccg atttctgccg acaagcacgc gatgcgcctg
     2221 cgcgagcaga tcgagcgcat tgcacgcgcc gagggcagca caccgggcac ggcgatctcg
     2281 caaatccgag agtcgatcgc gccgccgctg cgcgattcga tgcaggacct cctcgcgccg
     2341 tcaccgaatg actcgctatc gatgccgacg atgacggtgc tggccgacga cgtgctcgtc
     2401 gagtccgcac tcgaagctcc gtcgacggac gtcgtccccg agcccaagtc gtatcttcgc
     2461 gtcgagcccg agcgcgtcga tgcgctgatg tcgagcgccg gcgagctgct gttcgatcgc
     2521 acgcgcatcg agcggcgcgt ccagttgctc cgcacgttgg cgagagacct cgcacgcacg
     2581 cgtcaggatc tgcgcgatgc gatcgcgcgg gatgcgcccg gcaagacatc gctcggcgac
     2641 gccgaggccg agctcgcgag ccaggctgcg ctgctctcgc aaaccacggc ggcgctgctc
     2701 gacgagatcg aggcgctgcg ccgcacgatc ggtgatctgc agaagggtct gacgcggatc
     2761 cgcatggact ccgcgcgcaa cctcttcctc catgcggcgc gcacgctgcg cgcgttacgc
     2821 cg
//
LOCUS       DATLBZ010000489         5193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6275131, whole genome shotgun sequence.
ACCESSION   DATLBZ010000489 DATLBZ010000000
VERSION     DATLBZ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6275131"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VMZ53_24735"
     CDS             complement(<1..914)
                     /locus_tag="VMZ53_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31741.1"
                     /translation="MRSFVNAALLSLVVACGGESHQHTGIDALPSDTGGSDGLPPGCD
                     YLEQRDATNDDVFPSPGTPEATNLTIGNKTTICGGFQHTHFDGSITVDVDGYFATVAS
                     DADVLVRIAGTGADAVELVGVDVYPMGSTATAGKVTFYGDHGVASVHLAAGTYELLPF
                     ALNSTAITSTIDYKLELAAGSLDTRCALIPSGGYTEASDGTGSTGNDVVAFPTSGAPM
                     LTASGSDNPESTNVTVTATSNNRFSGSAADVTADKYEDKDTFAFVTGAANELTVRLGW
                     TGAANPDFFIFEAGSAAPVWRADGTTGTA"
     gene            complement(898..2415)
                     /locus_tag="VMZ53_24740"
     CDS             complement(898..2415)
                     /locus_tag="VMZ53_24740"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS31742.1"
                     /translation="MGGEQTLSGPDLEQGVSDGELREGVPLLGHAGGESVVLVRDGGT
                     VHAVGATCTHYSGPLAEGRVFGGAIHCPWHHACFDLKTGRASGPALAPIACFDVKLEG
                     GKIRVGAKRSPVTPDAKGPASVVIVGGGAAGVACAEALRDEGYSGPIWMVAGEGSDPV
                     DRPNLSKDYLAGSAPEEWVFLRSGDALAEKKITVVREAATAIDSKARVVKLASGKELA
                     FDALLLATGAEPIRLPLDGANLPHVHTLRTLADSKAIATATNEKTQAVIIGASFIGLE
                     AAASLRARGASVTVVGPESVPLARVLGDSVGAFIKGVHESKGVAFRLGRKPATITADK
                     VVLDDGSELPANLIVMGVGVKPRIELAASAGLQTDRGVVADDQLRAAPNIWVAGDIAR
                     YPYDGELVRIEHWQVAVRHGQAAARSMLGRPLRKDVPFFWSQHHDVTLGYVGHAEKFD
                     TPQVAGSLEARDAHVVYRNAGKVVAVLTLGRDQLALDVEAALERGDTSALDALVR"
     gene            2472..3068
                     /locus_tag="VMZ53_24745"
     CDS             2472..3068
                     /locus_tag="VMZ53_24745"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HUS31743.1"
                     /translation="MAVIELFFDIGSSYSYLASTQIDGVSQRTGVPVRWRPFLLGAVF
                     KATGNDLPIKLPAKARWMASDMAMWGKHYGIPVRIPSRFPMITLRAQRALCAAERLHG
                     PDGVPKFAQALFRAYWAEDKDPTADDVIGASAQTAGLEAAPIIAAIDAQETKDLLRAN
                     TDEAVKRGAFGAPSMFVGDTLFWGNDRLPLMEEFLKQS"
     gene            3065..3520
                     /locus_tag="VMZ53_24750"
     CDS             3065..3520
                     /locus_tag="VMZ53_24750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31744.1"
                     /translation="MKLAAALLVFAACEQSSGPPPPRPRLELVEAPGAGDVQTIVAEA
                     VVEATRDHKQLVVYEGAVWCEPCRDFHDAAAAGRLDATFGNVRMLVFDADRDGDALAQ
                     AGYRSELIPLFAIPRADGSSSGKQIEGAVKGQDAVAQITPRLQKLLADH"
     gene            3593..4066
                     /gene="dps"
                     /locus_tag="VMZ53_24755"
                     /gene_synonym="pexB"
     CDS             3593..4066
                     /gene="dps"
                     /locus_tag="VMZ53_24755"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HUS31745.1"
                     /translation="MYKTPSQLPEASRSTISAALNEILADGLDLHSQLKVAHWNIRGP
                     QFPTLHPLFEQFANQLAVHNDSIAERAVTLGGHALGTARSVAKQSRLAEYPAETTRDL
                     DHVRVLADRFESYLAAVRASRTSVEKAGDTDTVDLLTGVITEFEKNAWFLRATLA"
     gene            4143..>5193
                     /locus_tag="VMZ53_24760"
     CDS             4143..>5193
                     /locus_tag="VMZ53_24760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS31746.1"
                     /translation="MQAGADLTLIEASRRGDRDAFAEIIARYQRAVYAVAFAGVRDRA
                     LADDVAQDTFVIAWRRLSELRDASRLPAWLCGIARNRARELRRQTRREIASEMRDEIV
                     HPTTPYDAMSDAETERIIADALGKVPDVYREPLVLYYYEERSIDDVARSLGISPATTN
                     KRLSRGRRFLAESVATIERGLARRGPSPTLAASVLAVIGVTAPASHVDASPAAAKGSI
                     MHKLAIAALATITVGGAATAVAVTVARTDAHADTHTASSRKSVDSAHASAAGADGSHS
                     GSLCGLMAQLHGHKHAAAAPPSPLAALTTTVDANDCAAVGEHLANLQADATHGPNARP
                     DDTSFTTCSGQYTSMC"
BASE COUNT          874 a         1756 c         1684 g          879 t
ORIGIN      
        1 gcggttcccg tcgtgccgtc ggctcgccac acgggcgcgg cgctgccggc ctcgaaaatg
       61 aaaaagtcgg ggttcgcggc accggtccac ccaaggcgca cggtcagctc gttcgctgca
      121 cctgtcacga acgcaaaggt gtccttgtcc tcgtacttgt ccgcggtcac gtcggcggcc
      181 gacccgctga accggttgtt cgacgtggcg gtcaccgtga cgttggtcga ctccgggttg
      241 tcacttccgc tagccgttag catcggcgcg ccgctcgtcg gaaatgcgac cacgtcgttg
      301 ccggtgctgc cggtgccatc gcttgcttcc gtgtagccac ccgaagggat gagcgcgcag
      361 cgcgtatcga gagacccagc ggcgagctcc agcttgtaat cgatcgtcga cgtgatcgcc
      421 gtcgaattga gcgcgaacgg caggagctcg tatgtgcccg cggcgaggtg cacgctcgca
      481 acaccgtggt ctccgtagaa cgtcaccttg ccggcggtcg cggtggatcc catcgggtag
      541 acatcgacac cgacgagctc gaccgcgtcc gcacccgttc cggcgatccg aacgagcacg
      601 tcggcatcgg atgcaacggt cgcgaaatag ccgtcgacgt cgaccgtgat cgagccgtcg
      661 aagtgcgtat gttggaagcc accgcagatc gtcgtcttgt tgccgatggt caggttcgtc
      721 gcttcgggtg tgcccggcga cggaaacacg tcatcgttcg tcgcgtcgcg ctgctcgaga
      781 taatcgcagc ccggcggaag gccatcggag ccgccggtgt ccgacggcag cgcatcgatg
      841 ccggtgtgtt ggtggctctc gccgccgcag gcgacaacga gagagagcag ggcggcgtta
      901 acgaacgagc gcatcaagag cgctagtatc gcctcgttcc agcgccgctt cgacatctag
      961 tgcgagctga tcgcgtccga gcgtgagcac tgccacgacc ttgccggcat tgcgatagac
     1021 gacgtgcgcg tcgcgcgcct cgaggctgcc ggcgacctgc ggcgtgtcga atttctcggc
     1081 gtgaccgacg tagccgagtg tcacgtcgtg atgctggctc cagaaaaacg ggacgtcctt
     1141 gcggagtgga cggccgagca tcgagcgcgc agcggcctgg ccgtggcgaa ctgcgacttg
     1201 ccagtgctcg atgcgcacga gctcgccgtc atacggatag cgcgcgatgt caccggcgac
     1261 ccagatgttc ggcgccgcgc gcagctggtc gtcggcgacg acgccgcgat cggtctgcag
     1321 cccggcgctt gcggccagct cgatgcgcgg cttcacgccg acgcccatga cgatgaggtt
     1381 ggcgggcagc tcgctgccgt cgtcgagcac gaccttgtcg gccgtgatcg ttgcgggctt
     1441 ccggccgagt cggaacgcga cgcccttgct ctcgtgaacg cctttgatga acgcgccgac
     1501 gctatcgccg agcacgcgtg cgagcggtac ggattccgga ccgacgacag tcacgctcgc
     1561 gccacgcgcg cgcagcgatg ccgcggcttc gagaccgatg aagctcgcgc cgatgatgac
     1621 ggcttgcgtc ttctcattcg tcgccgtcgc gatcgccttg ctgtccgcga gtgtgcgcag
     1681 cgtgtggacg tgcggcaggt ttgcgccgtc gagcggtaga cgaatcggct cggcccccgt
     1741 cgcgaggagc agcgcatcga acgcgagctc cttgccgctg gcgagcttga cgacgcgggc
     1801 cttggaatcg atcgcggtcg ccgcttcgcg cacgacggtg atcttcttct cggcaagtgc
     1861 atcgccgctg cggagaaaga cccactcctc cggcgcgctg cccgccagat agtccttcga
     1921 gaggttcggg cgatcgaccg gatcgctgcc ttcaccagcg accatccaga tggggccgct
     1981 gtagccctcg tcgcgcagcg cttctgcaca cgcgacgcct gctgcgccac cgccgacgat
     2041 cacgaccgac gctggcccct tcgcatcggg tgtcacgggt gagcgcttcg cgccgacgcg
     2101 aatcttgccg ccttcgagct tcacatcgaa gcacgcgatc ggagcgagcg caggaccact
     2161 cgcgcgtccc gtcttcagat cgaaacacgc gtgatgccat gggcagtgaa tcgcgccgcc
     2221 gaacacgcgg ccttctgcga gtggcccgct gtaatgcgtg cacgtcgcac cgacggcatg
     2281 gacggtgccg ccatcgcgta cgagcacaac cgattcgcca ccggcatggc cgaggagcgg
     2341 cacgccttcg cgcagctcac catccgagac gccttgttcg agatcgggac cgctcagcgt
     2401 ttgttctccg cccatatcgc aacagagtct atccccgtcg ctggcgttac atctgctagg
     2461 ttggcgttcg catggccgtc atcgagctct ttttcgacat cggttcttcg tacagctatc
     2521 tcgcatcgac gcagatcgat ggtgtctcgc agcgaacggg cgtgccggtg cggtggcggc
     2581 cgtttctgct cggcgcagtc ttcaaagcga ccggcaacga cctcccgatc aagctgcccg
     2641 cgaaagcgcg atggatggcg agcgacatgg cgatgtgggg caagcactac ggcatcccgg
     2701 tgcgcatccc gagccggttc ccgatgatca cgctgcgggc gcaacgtgct ctgtgcgcgg
     2761 ccgagcggct gcacggtcca gatggcgtgc cgaaattcgc gcaagcgctg ttccgcgcgt
     2821 actgggccga ggacaaggat ccgacggccg acgacgtcat cggcgcctca gcgcagactg
     2881 ccgggctcga ggcagcgccg atcattgccg cgatcgacgc gcaagagacg aaggatctgc
     2941 ttcgtgcgaa caccgatgaa gccgtgaagc gcggcgcgtt cggcgcaccg tcgatgttcg
     3001 tcggcgatac gctgttctgg ggcaatgacc gcctgccgct gatggaagag tttctcaagc
     3061 agtcatgaag ctcgcggctg cgctcctcgt attcgccgca tgcgagcaaa gcagcggacc
     3121 gccaccgccg cgcccgcgtc tcgagcttgt cgaagctccc ggtgcgggcg acgtgcagac
     3181 gatcgtcgcc gaagcggtcg tcgaagcgac gcgcgatcac aaacagctcg tcgtttacga
     3241 gggcgcggtg tggtgcgagc cgtgccgtga cttccacgac gccgcagcag caggtcggct
     3301 cgacgcgacc ttcggcaacg ttcgcatgct tgtcttcgac gccgaccgcg atggcgatgc
     3361 gctggcacag gccgggtatc gctcggagct gatcccgctg ttcgcgatcc cgcgcgcaga
     3421 tggctcgtcg agcggcaagc agatcgaggg cgcggtgaaa ggccaggacg cagtcgccca
     3481 gatcacgccg aggcttcaga agctgctcgc agaccactga taggtacgtc ctatcagacc
     3541 catcgcaaga taggagctgt cctgatgacc ggggcggccg taggttgtct gcatgtacaa
     3601 gacgcccagc cagcttcccg aagcctcgcg gtcgaccatc tccgccgcgc tcaatgaaat
     3661 cctcgccgac ggtctcgatc tgcacagcca gctgaaggtc gcgcactgga acatccgcgg
     3721 cccgcagttc ccgacgctgc atccgctctt cgagcagttc gcgaatcagc tcgccgtgca
     3781 caacgattcg atcgcggagc gggccgtcac actaggtggt cacgcgctcg ggaccgcgcg
     3841 cagtgtcgcg aagcagtcgc ggctcgccga gtatccggcc gagaccacgc gcgacctcga
     3901 ccacgttcgc gtgctcgcag accgattcga gagctacctc gcggcagtcc gtgcgtcgcg
     3961 gacgtcggtc gagaaggccg gcgataccga tacggtcgac ctcctgaccg gcgtcatcac
     4021 cgagttcgag aagaacgcct ggttcctccg cgcgacgctc gcgtaggccc ctgaaatcgc
     4081 agcgactctg cgcgcgacga ttttcggtcc gaaacgcccg ccgtcttgca ctacaagacg
     4141 acatgcaagc aggtgccgac ctgacgctga tcgaggcaag ccgccgcggt gatcgcgacg
     4201 ccttcgccga gatcatcgcg cgctatcagc gagcggtcta cgcggttgca ttcgccggtg
     4261 tgcgcgatcg agcgctggcg gatgacgttg cgcaggacac gttcgtgatc gcgtggcggc
     4321 ggctatccga gcttcgcgac gctagccggc ttccggcatg gctgtgtggc atcgcgcgca
     4381 atcgtgcacg tgagctgcgc cgtcagaccc gtcgcgagat tgcgagcgag atgcgcgacg
     4441 agatcgttca cccgacgact ccgtacgacg cgatgagcga cgccgaaact gagcgcatca
     4501 tcgccgacgc actcggcaag gttcccgacg tctaccgcga gccactcgtc ctctactatt
     4561 acgaggaacg ctcgatcgac gacgtcgcgc gctcgctcgg catcagcccg gcgacgacga
     4621 acaagcgact ctcacgcggc cggcggttcc tcgcggaatc tgtcgcgacg atcgagagag
     4681 gcctcgcacg ccgtggcccg agtccgacgc tcgctgcgtc ggtgctcgcg gtcatcggtg
     4741 tcaccgcacc cgcttcgcat gtcgatgcct cacccgctgc tgcgaaagga tcgatcatgc
     4801 acaagctcgc aattgctgct cttgccacca ttaccgtcgg aggtgctgcc acggcggtcg
     4861 ccgtcaccgt cgcgcgcacc gatgcgcacg ccgacactca caccgcgagc tcgcggaagt
     4921 ccgtggactc cgcgcacgcg tcggcggccg gcgccgacgg atcccacagt ggatcgctgt
     4981 gcggcctcat ggcgcagctg catggccaca agcacgcggc tgcggcaccg ccatcgccac
     5041 tcgctgcgct gacaacgacc gtcgatgcga acgactgcgc tgccgtcggc gagcatctcg
     5101 caaatctcca agccgatgcg acgcacggac ccaacgcgcg ccccgacgac acgagcttca
     5161 cgacgtgcag cggccaatac acctcgatgt gcg
//
LOCUS       DATLBZ010000490         8468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1379023, whole genome shotgun sequence.
ACCESSION   DATLBZ010000490 DATLBZ010000000
VERSION     DATLBZ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379023"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2183)
                     /locus_tag="VMZ53_24765"
     CDS             complement(<1..2183)
                     /locus_tag="VMZ53_24765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HUS31747.1"
                     /translation="MPSPKQMPLGSIVKRKKRRLRTVSVLRLLFLLLIYGLSFAAACF
                     YFAVTTINDDLPQDLTKLLEYQPNRKSVVLSSDGEEIGSFSIENRRIIALDRMPPHVP
                     AAFLSAEDRRFFQHPGFDPVGIVRAAYKNFNSSGIKQGGSTITQQIIKQTLLSGEEGD
                     LSDLGMTAEQMTKEKKAQKYKRKFKEVILAYRLEKELTKAEILSIYLNHVYLGHGAYG
                     VGAAAETYFGKEVEDLTIAEAAMLAGLVASPTKYAPHRNMQLARERQKYVLGHMKEDK
                     YISEAEYEAALSEPIALVDESDINHLASPYFVEYVRKLATKRYGNSELFRGGLRFYST
                     LDTRMQQAAEDALRKGLESLDRRLGLRGPIGSVPLAQRGAWTGGPAHPLSGANDDTSA
                     LADQLLPEVRYGGMIVELGKGNSVVVDLGPKRLPLVDVDAKDVRAWHPTDKDDHVLWN
                     KGVQLGDLLPVRLNADGTALTVAQRPSLQGSLVAIEPSTGRVVALVGGYDWTASQFDR
                     ATQARRQVGSSIKPWIYAAGIENGKTPVDIMHDGPFSVTTATGVWTPANYDNKYMGNV
                     TLMTALAYSLNTISVQLVVFVGLDRVIEILRGFGIQSEIPRHISIALGTPDLTPLEIA
                     AGYAGIANGGRRVTPRFFDLVTNTTGDVVEDLRKAPPGPQVIKPEVAYVMINMMKGVV
                     TRGTAKYASTIGRPAGGKTGTSANYKDVWFNGFTTDLMVGVWVGR"
     gene            2198..4831
                     /locus_tag="VMZ53_24770"
     CDS             2198..4831
                     /locus_tag="VMZ53_24770"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31748.1"
                     /translation="MRSGRWPHVLSLIAATALAQPSDTAAPTPTPATAPESLDVPDWK
                     AGPSKVAVTPFENHVTNGKSLEWMVAETPFEIAEKSENVLGLDAVNPPLYVPGERVPA
                     DPDTVADFGHKMGAEYVVTGWYDRIREQLRIAVLVWKVDKGPNAKLAGESQKLGAPDD
                     YHKILGEAMGDAWSKAGVGVDLARSEQLQRPLAKDTYPVFMMGRGLGHFSGALAAMNA
                     IYGTGSGSGATGPDYESAKHDLERAVFLDPKLAEAQRLLGELYMQTAAGDPKLISKAT
                     GKFTYAADLAPDDVSSLRAAAFAMARAGKSEVALDMFKKIVTKHPWDLDARYELGAAL
                     WHVGEAKSAEHQLEQVTAHQPDHLQARRVLVLIHSSRNDTPKLISELEAIQQRAPNDL
                     EIKGDLATAYGAVGKWPKAIAELEAIATARPNDLALLVRIGHAYRKNNQTDKALEWYA
                     RASKVEPDSSYPGYAAAQAQFDAGKLDEAFRAYTLIQKFTVDRGAAEHAMGVIAMLQG
                     KTDQAAWNLRQAVRTEPRSLATRRAVIAAEINRKDAAAALQQAEPALVGWPNDGTLHY
                     LAGVAHALAGETDEARDELNKALQLQPDLAAAKSGLSALAAGTTPTLEFQPELVRPWG
                     DGDALQVVIDDYARVEQAMIAARADYQKAFLALLGAVGQGPKARVKPGSVKVCPAGQI
                     APDWDAAQKALQRYTRLGVELESSWRFIARHDEIGMTASLLPNSRTAVAGARKSYKLA
                     LADVAELRAEWTRGVTPELRTAGCNDKLLAAALKDPARYKMIAEDKPDQLPPQLAPRA
                     KPRSTFFVDNTRCPDPVDVWIDGSHVGQVAPGRRSALVSDGGERTLCLLTPGGAQCGD
                     RGTVRQVYLHDGWSTTLYCPK"
     gene            4847..5671
                     /locus_tag="VMZ53_24775"
     CDS             4847..5671
                     /locus_tag="VMZ53_24775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31749.1"
                     /translation="MRRLLVVAFLAGCHSAPEPAKNTPSTWSEATFDTNVQAVLAKHF
                     DGKSIKKLEPDLYRVGDFEVRTSKARSECREDWAHCDAAVGRTVAAIDQLRHAEPARR
                     DQLRVILRSNDKLSAVKERMKGVITQPFSSDAQWVLAVDMPNVIGYDVDLGALGLSAD
                     EAWKLATANTKPGKLVTAKSGGGSIVYQDAYAPSALRYPELLEAAARKEAPDRRGNLL
                     AVTPEENILLFTVGGDLEAGMLRDAATNGIKNSEMPLSTHVMEWTGRSWRERADAQ"
     gene            complement(5665..7212)
                     /locus_tag="VMZ53_24780"
     CDS             complement(5665..7212)
                     /locus_tag="VMZ53_24780"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31750.1"
                     /translation="MRRCWFLAFAAIAGCGRFGFGDVDHRADSAVTNGDGDITGDGGA
                     TLRFSYLKPMNTETFDRFGFAIALSADGKTLAVGAPAEDCGIVGVNASDGSNNGVSQS
                     GAVYIFARSGPGWVQQAYIKPSTVDINDAFGAALALSGDGNTLAVGVPNEDSAARTIG
                     GTESDNSALESGAVFVFARTNTTWSQQAYIKSSNSDAGDEFGWAVALTGTGDMLAVGV
                     PQEDGGSMGGGGDPSSNTAMDSGAVITFTRSGATWTQDAYIKPSNTGAGDLFGNTLSI
                     AEDGATLVVGAPAEDSGATIAIGSSTVGSQLDNSVTEGGAAYIFVLGAGMWYQQGYLK
                     PDKIDFGDFFGKVVSISSDGNTVAAASPGEDTIANSGAVSTFTRSADIWTAQQKLKAA
                     NAGDNDQFGVSLALSGDGTKLMVGAPFEDSNGISLGGNPNDNSSSDSGAVYAFVRGAS
                     WSQTSYSKGNAPDPGDQWGYAVAISRDGTTRSVSAAYEDSAATGINGNALDNTAQDSG
                     CAGVSNY"
     gene            7313..8197
                     /locus_tag="VMZ53_24785"
     CDS             7313..8197
                     /locus_tag="VMZ53_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31751.1"
                     /translation="MLPKPDKLPGRVVVLDIAFAATVGSSVSFSLTTQPFITGLGDRL
                     AAWVDHHDHERHAEFAGDPRFVLSTKAEHGACPEMVTPDLVRTTGPIDTIVTHVDLDG
                     LYAAAKWILDGVEPYKGADEDARAVDTRVGTPGPIATRIDKALRAKFRDDQLKRAVVH
                     FLVGGMKSGPHTEVIDDAAAEFDRRDAKTRELAQRFTIRGRVAIVDTAGAAGQFDKTD
                     LLLAGQAKAPVSIVRDSGMLTIAAPFDSGWDFVAIFELGGGMPTRVTIPEQKLDAVIA
                     KINDAPEPTPRQTSPVED"
     gene            8283..>8468
                     /locus_tag="VMZ53_24790"
     CDS             8283..>8468
                     /locus_tag="VMZ53_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HUS31752.1"
                     /translation="MALQLGDEAPNFTAETTEGTVNLHEYLGSGWGVLFSHPKDFTPV
                     CTTELGRVSALKPEFDKR"
BASE COUNT         1451 a         2732 c         2837 g         1448 t
ORIGIN      
        1 tcgcggccga cccagacgcc gaccatgaga tccgtcgtga agccgttgaa ccagacgtcc
       61 ttgtagttcg cgctcgtgcc cgtcttgccg ccggcggggc ggccgatcgt cgacgcgtac
      121 ttcgcggtgc cgcgcgtgac gacgcctttc atcatgttga tcatcacgta cgcgacctcg
      181 ggcttgatga cctgcgggcc gggcggcgcc ttgcgcagat cctcgacgac gtcgccggtc
      241 gtgttcgtca cgagatcgaa gaaccgcggc gtcacgcgcc ggccgccgtt cgcgatgcct
      301 gcgtagccgg ctgcgatctc gagcggcgtg aggtcggggg tgccgagcgc gatcgagatg
      361 tggcgcggga tttcggattg gatgccgaag ccgcggagga tctcgatcac gcgatcgaga
      421 ccgacgaaca cgacgagctg cacgctgatc gtattgagcg agtacgcgag cgcggtcatc
      481 agcgtgacgt tgcccatgta cttgttgtcg tagttcgcag gcgtccacac gccggtggcc
      541 gtcgtcaccg agaacgggcc gtcgtgcatg atgtcgacgg gcgtcttgcc gttctcgatg
      601 cccgccgcgt agatccacgg cttgatcgac gagccgactt ggcgacgcgc ttgggtcgcg
      661 cgatcgaact gcgacgcggt ccagtcgtag ccaccgacga gcgcgacgac gcgacccgtc
      721 gacggttcga tcgcgacgag cgagccttgc agcgatggtc gctgcgcgac cgtgagcgcg
      781 gtgccgtcgg cattgaggcg cacgggcagg aggtcgccga gctgcacgcc tttgttccag
      841 aggacgtgat cgtctttgtc cgtcgggtgc cacgcgcgga cgtctttggc gtcgacgtcg
      901 acgagcggca gacgtttggg gccgaggtcg acgacgacgc tgttgccttt gccgagctcg
      961 acgatcatgc cgccgtagcg aacttcgggg agcagctgat cggcgagcgc gctcgtgtcg
     1021 tcgttcgcgc cggagagcgg gtgtgcgggc ccgccggtcc atgcgccgcg ctgcgcgagc
     1081 gggacgctgc cgatcggacc gcgcagaccg aggcggcggt cgagcgactc gaggcctttg
     1141 cgcagcgcgt cctcggcggc ttgctgcatg cgcgtgtcga gcgtcgagta gaagcgcagg
     1201 ccgccgcgga acagctcgct gttgccgtac cgcttcgtcg cgagcttgcg gacgtactcg
     1261 acgaagtacg gcgacgcgag gtgattgatg tccgattcgt cgacgagcgc gatcggttcg
     1321 gagagtgcgg cttcgtactc cgcttcggag atgtacttgt cctccttcat gtgaccgagg
     1381 acgtacttct ggcgctcgcg cgcgagctgc atgttgcgat gcggtgcgta cttcgtcggt
     1441 gacgcgacga ggccggcgag catcgcggcc tctgcgatgg tgaggtcctc gacctctttg
     1501 ccgaagtacg tctccgccgc cgcgccgacg ccgtatgcgc cgtggccgag atagacgtgg
     1561 ttcaggtaga tggagaggat ttccgccttc gtcagctcct tctcgaggcg gtaggcgagg
     1621 atgacctctt tgaacttgcg cttgtacttc tgcgccttct tctccttcgt catctgctcg
     1681 gcggtcatgc cgagatcaga gaggtcgcct tcttcgccgg agagcagcgt ctgcttgatg
     1741 atctgctggg tgatcgtcga gccgccctgc ttgatgcccg acgagttgaa gttcttgtag
     1801 gccgcgcgga cgatgccgac gggatcgaag ccgggatgct ggaagaaccg gcggtcctcg
     1861 gcggagagga acgcggccgg cacgtgtggc ggcatgcgat cgagcgcgat gatgcggcgg
     1921 ttctcgatcg agaacgaccc gatctcttcg ccgtcgctgg agagcacgac ggactttcgg
     1981 ttcggctgat actcgaggag cttggtcagg tcctgcggta ggtcgtcgtt gatggtcgtg
     2041 accgcgaagt agaagcaggc ggccgcgaat gaaagcccat agatgagcag caggaatagg
     2101 aggcgcagca ccgacacggt gcgcaaccgg cgcttcttcc gcttgacgat cgagccgagc
     2161 ggcatttgct tcggcgaagg cacgctacaa tgagtctatg aggtctggcc gctggccgca
     2221 cgtcctctcg ctgattgctg cgactgcgct cgcacagcca agcgatacgg cagcgcctac
     2281 accgacgccg gcgacggccc ccgagtcgct cgatgtcccc gactggaagg cggggccctc
     2341 aaaagtggcg gtgacgccgt tcgagaatca cgtcacaaac ggcaagagcc tcgagtggat
     2401 ggtcgccgag acgccgttcg agatcgccga gaagagcgag aacgtgctcg gtctcgacgc
     2461 cgtcaatccg ccgctctacg tgcccggcga gcgcgtaccg gccgatcccg acaccgtcgc
     2521 ggacttcggt cacaagatgg gcgccgagta cgtcgtcacc ggttggtacg atcgcattcg
     2581 cgagcagctg cgcatcgcgg tcctcgtgtg gaaggtcgac aaggggccga acgcgaagct
     2641 cgccggcgag tcgcagaagc tcggcgcgcc ggacgactat cacaagattc tcggcgaggc
     2701 gatgggcgac gcgtggtcga aggccggcgt cggcgtcgat ctcgcgcgga gcgagcaact
     2761 ccagcgaccg ctcgcgaagg acacgtatcc ggtcttcatg atgggacgcg ggctcggtca
     2821 cttcagcggc gcgctcgcgg cgatgaatgc gatctacggg acgggctcgg gttcgggtgc
     2881 aacggggccc gattacgaat cggcgaagca cgatctcgag cgggcggtgt tcctcgatcc
     2941 gaagctggcg gaagcgcagc gcttgctggg cgagctctac atgcagacgg ccgcgggcga
     3001 tccgaagctg atctcgaaag cgacgggcaa gttcacgtac gccgcggacc tcgcgcccga
     3061 tgacgtgtcg tcgctgcgag ccgcagcgtt tgcgatggcg cgtgccggca agtccgaagt
     3121 cgcgctcgac atgttcaaga agatcgtcac gaagcatcca tgggatctgg atgcgcgcta
     3181 cgagctcggc gcagcgctgt ggcacgtcgg cgaggcaaaa tcggcggagc accagctcga
     3241 gcaagtcacc gcgcaccagc cggatcactt gcaagcgcgt cgcgtcctcg tcctgatcca
     3301 cagctcgcgc aacgacacgc caaagctcat cagcgagctc gaagcgatcc agcagcgcgc
     3361 cccgaacgat ctcgagatca aaggggacct cgcgacggcc tacggcgcgg ttggcaagtg
     3421 gccgaaggcg atcgcagagc tcgaagcgat cgcgacagca cggccgaatg acctcgcgtt
     3481 actcgtccgc attggccacg cgtatcgcaa gaacaaccag accgacaaag cgctcgagtg
     3541 gtacgcgcgt gcgtcgaagg tcgaacccga ctcgagctac ccgggctacg ccgcggcgca
     3601 agcgcagttc gacgcaggca agctcgacga agcattccgc gcgtacacgc tgatccagaa
     3661 gttcaccgtc gatcgcggcg ccgccgagca tgcgatgggc gtgatcgcga tgttgcaggg
     3721 caagaccgat caagccgcgt ggaacctgcg ccaagcggtg cgcacggagc ctcgctcgct
     3781 cgcgacacgc cgcgcggtca tcgccgccga aatcaatcgc aaggacgccg ccgccgctct
     3841 ccagcaagca gagcctgcgc tcgtcggctg gccgaacgac ggcacgctgc actacctcgc
     3901 aggtgtcgcc catgcgctcg ccggcgaaac cgacgaggct cgcgacgagc tcaacaaagc
     3961 gctgcagctc cagcccgatc tcgcggccgc gaagtcaggt ctgagcgcgc ttgctgccgg
     4021 caccacgccc acgctcgagt tccagcccga gctcgtccgt ccatggggtg acggcgacgc
     4081 gctgcaggtc gtgatcgacg actacgcacg cgtcgagcaa gcgatgatcg cggcgcgcgc
     4141 ggactatcag aaagcatttc tcgcgctgct cggcgcggtc gggcagggac ccaaagcgcg
     4201 cgtcaaaccc ggctccgtca aagtctgtcc cgctggccag atcgcaccgg attgggacgc
     4261 ggcgcaaaaa gcgctgcagc gctacacgcg actcggcgtc gagctcgaga gcagctggcg
     4321 cttcatcgcg cgtcacgacg agatcggtat gacggcgagc ctattgccga actcgcgcac
     4381 cgcggtcgca ggcgcacgca agagctacaa gctcgcgctt gccgatgtcg cggagcttcg
     4441 cgctgaatgg acgcgcggcg tgacgcccga gctgcgcacc gcgggctgca acgacaagct
     4501 gctcgcggct gcgctgaaag atccggctcg ctacaagatg atcgcggagg acaagcccga
     4561 ccagctgccg ccgcaattgg ctccgcgtgc gaagccgcgc tcgacattct tcgtcgacaa
     4621 cacgcgctgc cccgatcccg tcgacgtgtg gatcgatggc tcgcacgtgg ggcaggtagc
     4681 gcccggtcgc cgcagcgcgc tcgtctccga cggtggtgaa cgaacgctgt gcctcctcac
     4741 accaggcggc gcgcagtgcg gcgatcgcgg caccgtgcgc caggtctacc tgcatgacgg
     4801 ctggtcgacg acgctctact gcccgaagta atgatatgag cgcgcgatgc gcaggctgct
     4861 tgtcgtcgcc ttcctagcgg gctgtcactc ggcgccggag ccggcgaaga acacccccag
     4921 cacgtggtcg gaagcgacgt tcgacacgaa cgtccaggcg gtgttggcca agcacttcga
     4981 cggcaagtcg atcaagaagc tggagcccga cctctatcgg gtcggcgact tcgaggtgcg
     5041 gacgtcgaag gcgcgcagtg aatgccgcga agattgggcg cactgcgatg ccgctgtcgg
     5101 ccgcacggtc gccgcgatcg atcagcttcg gcacgctgag ccagctcgtc gcgatcagct
     5161 acgcgtgatt ctgcgatcga acgacaagct gtccgcagtg aaggagcgca tgaagggcgt
     5221 catcacgcag ccgttctcgt cggatgcgca gtgggttctc gccgtcgata tgccgaacgt
     5281 gatcggctac gacgtcgacc tcggtgccct cgggctcagc gcagacgaag cgtggaagct
     5341 cgcgaccgcg aataccaagc ccggcaagct cgtcaccgcg aagtcgggcg gcggctcgat
     5401 cgtctaccaa gacgcgtatg cgccgagcgc gctgcgctat cccgagctgc tcgaggccgc
     5461 cgcacgcaaa gaggcgccgg atcgcagggg aaacctgctc gcggtcacgc ccgaggagaa
     5521 catcctgctc ttcaccgtag gtggggacct agaagctggg atgctgcgcg atgccgccac
     5581 gaacgggatc aagaacagcg agatgccgtt gtcgacgcac gtgatggagt ggaccggccg
     5641 cagttggcgc gagcgcgctg acgctcagta gttcgagacg cctgcgcagc cgctgtcttg
     5701 cgccgtgttg tcgagcgcat ttccgttgat gccggtcgca gcgctgtcct cgtacgcagc
     5761 ggataccgac cgcgtcgtcc cgtcgcgcga gatcgcgacg gcgtagcccc actggtcgcc
     5821 ggggtcgggc gcgttgccct tcgagtacga cgtctggctc caactcgcac cgcgcacgaa
     5881 cgcgtagaca gcgccgctgt cgctgctcga gttgtcgttt ggattgccgc cgagcgagat
     5941 gccgttgctg tcctcgaagg gcgcaccgac catcagcttc gtgccgtcgc ccgagagagc
     6001 gaggctcacg ccgaactgat cgttgtctcc cgcgttcgcg gccttgagct tttgttgcgc
     6061 cgtccagatg tcggccgagc gcgtgaacgt ggacaccgcg ccgctgttcg cgatcgtgtc
     6121 ttcgccgggt gatgccgcgg caactgtatt gccatcgctg ctgatcgaga cgaccttgcc
     6181 gaagaagtcg ccgaaatcga tcttgtccgg tttcagatag ccctgctggt accacatgcc
     6241 ggctccaagc acgaagatgt aggcggctcc gccttcggtc acgctgttgt cgagttgcga
     6301 gccaacggtc gatgatccga ttgcgatcgt tgcgccgctg tcttccgcgg gtgctccgac
     6361 gacgagcgtg gcgccatcct ccgcgatcga gagcgtgttg ccgaatagat cgccggcccc
     6421 agtgttcgag ggcttgatgt acgcgtcttg cgtccacgtc gcgccgctgc gcgtgaacgt
     6481 gatcaccgcg ccgctgtcca tcgcagtgtt gctgctcggg tcgccgccgc cacccatcga
     6541 gccgccatct tcttgcggaa cgccgacggc gagcatgtcg cccgttcccg tcagcgcgac
     6601 agcccacccg aattcgtcgc cggcatcgct gttggacgac ttgatgtacg cctgctgcga
     6661 ccacgtcgtg ttggtgcgcg cgaatacgaa cacggcaccg ctctcgaggg cgctgttgtc
     6721 gctctctgtg ccgccgatcg ttctcgccgc gctgtcttca ttcgggacgc cgaccgcgag
     6781 cgtgttgccg tcgccggaga gagcgagcgc tgcaccgaac gcatcgttga tgtcgacggt
     6841 cgacggcttg atgtacgcct gctgcaccca gcctgggccg ctgcgcgcga agatgtagac
     6901 ggcgcccgat tggctgacgc cgttgttcga gccgtcgctc gcattcacac cgacgatccc
     6961 gcagtcctcg gcgggcgcgc caacggcgag cgtcttgccg tcggctgaca acgcgatcgc
     7021 aaagccgaag cgatcgaacg tctcggtgtt catcggcttg aggtagctga agcgcagcgt
     7081 cgcgccgccg tcgccggtga tgtctccatc gccattcgtg accgcggagt ccgcgcgatg
     7141 atcgacatcg ccaaacccga agcggccgca gccggcgatg gcggcgaacg cgaggaacca
     7201 acaccgcctc actgcgtcga tcgtatcagg gtgaagcgcc gccccgagca gcttctagtg
     7261 ctacggtctg cgaacgttgc gtttgccgca tttggcgttt gcatcgccgc gattgctccc
     7321 aaaacccgac aagttgccgg gtcgcgtcgt cgtgctcgat atcgcattcg cggcgacggt
     7381 cggctcgagt gtgtcgtttt cgctcactac acagccgttc atcacgggcc tcggcgatcg
     7441 gttggccgca tgggtcgatc atcacgacca cgagcggcat gcggagttcg cgggcgatcc
     7501 gcgcttcgtg ctgtcgacga aagccgagca cggcgcgtgt cccgagatgg tgaccccgga
     7561 tctcgtgcgc acgacggggc ccatcgatac gatcgtgacg cacgtcgatc tcgacggtct
     7621 ctacgccgcg gcgaaatgga tcctcgatgg tgtcgagcca tacaaaggcg cggacgagga
     7681 cgcgcgagcg gtcgatacgc gcgtcggcac gccggggccc atcgcgacgc gcatcgacaa
     7741 agcattgcgc gcaaagtttc gcgacgacca gctcaagcgc gcggtcgtgc atttcctcgt
     7801 cggagggatg aagtccggcc cgcacaccga ggtgatcgac gacgctgccg ccgagttcga
     7861 tcgccgcgat gcgaagacgc gcgagctcgc ccagcgattc acgattcgcg gccgcgtcgc
     7921 gatcgtcgat acagcggggg ctgctggtca gttcgacaag acggatctct tgctcgcagg
     7981 gcaggcgaag gcaccggtct cgatcgtgcg cgactccggc atgctcacga tcgcggcgcc
     8041 gttcgactcg ggctgggact ttgtcgcaat cttcgagctc ggcggcggga tgccgacgcg
     8101 cgtgacgatt cccgagcaga agctcgatgc cgtgattgcg aagatcaacg acgcgcccga
     8161 gccgacgcct cgacaaacgt cccccgtcga ggactgagcc ttccaatccg tgcaagtgtc
     8221 gcttgccgac ccatagcgcg tccgctataa cggcagcctc gataacatca cggagaatcg
     8281 ccatggcact gcagctcgga gacgaagcac cgaatttcac ggcagagacg accgagggca
     8341 ccgtcaacct gcacgagtac ttgggcagtg gttggggcgt gctgttctcg cacccgaaag
     8401 acttcacgcc ggtatgcacg accgagctcg gtcgcgtctc ggcgctcaag cccgagttcg
     8461 acaagcgc
//
LOCUS       DATLBZ010000491        23574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8518480, whole genome shotgun sequence.
ACCESSION   DATLBZ010000491 DATLBZ010000000
VERSION     DATLBZ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 23574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23574
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8518480"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VMZ53_24795"
     CDS             complement(<1..336)
                     /locus_tag="VMZ53_24795"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HUS31753.1"
                     /translation="MVRVWLVAAVAIAGCVHASAAPREKPADFVDVPTLIPDAVLDIR
                     YATDDNFTGKAVYPVAHCKLRRSVAARLVKAAAFLRKSDRRLLIWDCYRPKSIQDVFW
                     KLVPDERYVA"
     gene            364..1347
                     /locus_tag="VMZ53_24800"
     CDS             364..1347
                     /locus_tag="VMZ53_24800"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HUS31754.1"
                     /translation="MSTDARNDGLIDAFLFDGHGGARVLDWDGIDAWRADAGMLWLHV
                     DYANPAVRQWLDTKSGIDDVILSALTDPDPRPRAIAYGDKLQLIVRGMNNNVGSAPEN
                     MISVRAWVEPRRVITMRHRASTSVKQLVSELKQGEGPRNAADLTVQLAERIVDHVVAR
                     VDRLGDAVAACEDQVLGGDAGDLRATLADHRRNAIALRRYLGPQRDALTKLASINVPW
                     LEPNHRARLAEVADQLTRTVEELDAARDRAAVTQEELGSRVAEATNKRLYVLSIFTSV
                     FLPLGFVCSLLGVNVGGVPLQKSDWAFWALCGVFALYIYVQLWLFKKRGWL"
     gene            complement(1348..2835)
                     /locus_tag="VMZ53_24805"
     CDS             complement(1348..2835)
                     /locus_tag="VMZ53_24805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31755.1"
                     /translation="MRSALLWLAVAACASGNGSTVDSGTSSGDSSAPMIDAASCGNLP
                     CNAVYVSRMGNDSSAGTKMAPMKTINAAITKALNTVPPLAVFVEGGTYPEQVAMKAGV
                     AVYGGFDATWTRTPAVVTEINAASPAVTFDQIQTGAAIDGVTIKSTDATGAGASSYAV
                     VITSSKLIELRDVTITPGIGAKGNDGGNGSVGAMGGAGGNGNPGAEYSTGFGCDVHAL
                     PAVGTSGASSCGRTGGLGGAAGVGDSAGNTGGDSAGLAQSGGFGGGGGGHNPPCGGAG
                     DGQVGADGFPGSAGSNGAAGMDIGTFNGVMYIPSGGGDGLNGTAGYGGGGGGGGGGGT
                     ASCDSSGSSGGGGGGGGCGGGRGFAGTGGGGSFGVIAVDSSVAIKSSMVIANRGGDGG
                     RGGQGGSGGTGGGYGLGGVYSNSSEQDDGGCGARGGLGGAGGGGGHGGGGGGGPSAAL
                     VCLGTASIQIPQTTAMGATGGSGGTSPGLAGASGVSTRAIDCSFF"
     gene            complement(2882..3772)
                     /locus_tag="VMZ53_24810"
     CDS             complement(2882..3772)
                     /locus_tag="VMZ53_24810"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HUS31756.1"
                     /translation="MSFADLRADTAVRADDSVPGRYHLTLPDHWDYLLPSGGVVMTAA
                     LRAAQAHIGDPTFRLASATTIFASPIHPGKCLADVHVIRRGNSAVQTRVIFRHTQEKD
                     GEPGLELIATFMRDRKGPDVACVPFPAVKSLADALPVDDGASNNPHTRFRFYHQLECK
                     LADGPAFWQPDFAAGPARYARWFRYRAPQRDAEGRLDRLAIPPLIDTMPTALHRAIGP
                     GGYRFYAPSLDLTVYTIDDTMREWLLVAVTMRRALGGWAIADAEVWDDAGKLIAYGSQ
                     AMYLHGVSGDPPTIDAARRA"
     gene            complement(3797..4558)
                     /locus_tag="VMZ53_24815"
     CDS             complement(3797..4558)
                     /locus_tag="VMZ53_24815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31757.1"
                     /translation="MHFPKYWARGQATSGSSSASCWRWSDVSEDDARQKANQRAAELA
                     ALFASGRRLDRYSYGDRPLREEIVQRLDNGVVITRNLYGALVLNAERAMFIDVDFGDG
                     AGEGAAIQRVQQWTASHPELAVRVYRTHAGLRLLVTNHTYDPKSAEAIALLEEVGTDK
                     LYIQLCKAQASFRARLTPKPWRIGMKEPRVRFPYESAEQHGELLHWVSHYELASKKAS
                     VCKLLETTGPASVLDEIRPLLAYHDHVAVGDQPLA"
     gene            complement(4599..7037)
                     /locus_tag="VMZ53_24820"
     CDS             complement(4599..7037)
                     /locus_tag="VMZ53_24820"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HUS31758.1"
                     /translation="MSEKSSKWVVFHGHFYQPPRENPWLEAIEPQPSAHPYRDWNERI
                     TAECYRPNTAARVIDGQGHIIQIVDNYERMSFNVGPTLMSWLERHAKDVHESLVAADR
                     ASRERFSGHGSAMAQAYNHIIMPLASPRDKVTQVRWGIADFKRRFHRAPEGMWLPECA
                     VDTASLEALAAEGILFTVLAPHQAKAWRPPGGEWRTTPVDPGRVYKYKLPSGRSIDLF
                     FYDGATSQAVAFERLLSDGHHIISRMTNRGPVEGDGPTLCHIATDGETYGHHHRYGDM
                     ALAWALSQVEQGWNGTRLTNYAEFRTKFPATWEVQLLENSSWSCIHGIERWRNDCGCN
                     SGGKPGWNQKWRRPLRDAFDWLREQASGALDSVGRALFKDPWVARDAYVDALLNGRQG
                     RDRFLETHASHSLSTEERVRALSLMEMARHEMLMYTSCGWFFDDLSGIETVQCIQYAA
                     RVAELLNSISGVSVESELVERLSLARSNVPDHGDGRRVWTMFVRPARVDPSKVAAHVA
                     VHALVEPDPTRAVAVDGFHVEFLDRVERKSGRARMVAGTVCVKSSLTDAATALCFAGL
                     HLGEQHVTGGVRVPPSESEWAHMLKELRDSLETGDVFAAQRAIDRHFPGAHLSLGALL
                     PGSRERVLAAVLGEAIGSAHEALVDTYDQHAPLIRWLVAHDLPVPEALHTVAEASLRQ
                     RVLANLSLENASFQALRDHIAEATEVKVSLDTPEIALAASEGLRRLIDRVAPSKDSEI
                     DFAALDTVTRAAEVAARMKSSVDLWFAQNATWRLLQRLPELRARGKSGDINALTAVSD
                     LERLARALRLAVPA"
     gene            complement(7034..8929)
                     /gene="glgB"
                     /locus_tag="VMZ53_24825"
     CDS             complement(7034..8929)
                     /gene="glgB"
                     /locus_tag="VMZ53_24825"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HUS31759.1"
                     /translation="MTQPFQKPAAQSLLTSDDLFLFNQGRHFKLYEHLGAHPVEGGTF
                     FAVWAPSARSVSVIGDWNGWVHGSDKLRPRESSGIWEGVLKGVGHGTRYKYAIVGPDG
                     ATHEKADPFAARYEHPPMTASIVWKPQHQWKDGDWMKSRGERAARNAPMSIYELHLGS
                     WRRRNGEVLGYRELGQQIAHHVTQLGFTHVELMPVMEHPFYGSWGYQVTGYFAPTTRY
                     GAPEDFMCFVDELHQRGIGVILDWVPAHFPTDQHGLGYFDGTHLFEHADPRRGFHPDW
                     RSYIFNYARHEVRSFLISSAINWLDRYHIDGLRVDGVASMLYRDYSRQAGEWVPNEDG
                     SNHDRDAIHFLQDFNKAVYTMFPDVQTIAEESTAWGGVSRPPEYGGLGFGYKWDLGWM
                     HDTLKYLEREPIHRQWHHGEITFRAIYQNSENYCLPLSHDEVVHGKGSILSKMPGDPW
                     QKYASARLLYGYQWTTPGKKLLFMGSEIGVWNEWNHDQELDWGCGMHPAHAGIARWVG
                     DLNSAYRKYKSLHVGDCDPVGFRWLVGDDSGSSIIAYLRTGNGGDPPALVVANFTPVP
                     REGYRLGVPKGGFWREILNSDASIYGGSGVGNRGGMHTDGQGWHGYDQSLVVTVPPLG
                     IVVFVPE"
     gene            8996..9334
                     /locus_tag="VMZ53_24830"
     CDS             8996..9334
                     /locus_tag="VMZ53_24830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31760.1"
                     /translation="MPKATVRNRRNVLPIRGRKEADKTMRKMMMVALFSVLSLSACAV
                     EEPVQTAQDEAPAEQQPCNTADDHHARPTNARELEEEGEAVWLQCDDNSECESNVCDL
                     EIHTCREYPF"
     gene            9475..11307
                     /locus_tag="VMZ53_24835"
     CDS             9475..11307
                     /locus_tag="VMZ53_24835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31761.1"
                     /translation="MMLVASLMSAACGSVEEPAVMNDDGTGSDVGSGSGSGSGSGSAS
                     PDAGVPVDTGCMKAADCGANMACDIATHACVAGALTIDPSDFVIDSGVWWTTTSGPVL
                     HGTFQGPTSAVVEVKTGASQPVMATLTGNAWAVTLPAGTISSSYAPIVVMLTDPSGSD
                     IELARTLGLDSAPPAITVQGSIRDERNDTIDFATGEPVHTHAGAATDLASTGCASVYK
                     YAYLMDASPAFGSATSPNDLAWKLRITDAKLQTGSQQFRVRTDAGAVLINWSALPTPD
                     AAGTSTVKLTRSGGAYAIAALGTRTGQFFIDVRATDWGGLSSTASFCFDNHPLAAPLS
                     ISAIEKGDLFGLSTTNDAGIAALFADNAAVNVVKQHIVQQVAEPISFTWSLQATSPSY
                     TAQQVWGYVEETFNGGYMCQPGNSKPECVMTTKPSSSYPPASASPSGGTLASSYSVAL
                     RDDVTGQIVATSTTKSITWTIPARGSSEAPHGYTVEVAQSGFYAIRPLSVFWYGYWTD
                     SPVGVSTLMGSTYTGTAPYDIKGTQCVQMIQTAFGPMCQNAIKYWGMSAIDQVTLSYS
                     PFVATFSTGATATQLSAPSYVMPSMLTSQAFTWDSGDDDLPGAL"
     gene            complement(11722..15099)
                     /locus_tag="VMZ53_24840"
     CDS             complement(11722..15099)
                     /locus_tag="VMZ53_24840"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HUS31762.1"
                     /translation="MRRIAIEKIDEAPVLAEIAADETERSLREFAGERAAQLYASTAC
                     KENEDAASNALSGITKLGDQHALIDVAVHAKLSGIRKKAFEELRDPKALAELAKDSDA
                     PQDIRAAAIARIDDGETLRAIAIDTTIKETGLAAVEKLDDVERLENVANKAKNKAVRQ
                     RARKIVQEITASEKAKQKPSVPDDVKRRRAEKAQLVRTLEGLADTFDFAKSEGVVKAA
                     QESWSKLEGADEGDERFTKAVERFWKRKELHDQQARSADELRAVEREAQRERERAAAE
                     RAAREAQAKSPESTEAKPDPAAADAEREERQKRRAEEDARRAEEQAAREARRKEDAER
                     GAAIATSLSAMLDDMEKLAAGSDRDTRGIDRLLQQAGRSFEQIGKVPADQRESLSDRY
                     TKARGKLVVRASELREAEDWERFANVPKAEALIATAKDMLAEESSPDLGNRLRQLQAL
                     WKEVGPMPQRRSKELWESFKSLCDQIYDKVKGFRAVESEKFADVTKAKEALIAEAESL
                     AESTDWQATANRLKELQAAWKQSGHLPRKQGDDLWKRFRAACDRFFERRKPQLDARNA
                     EEAQNLSAKQALIARAQTVANNAPGEGGWGKAIAEIKNLQQQWKEIGFVPRRDADAVY
                     KAFRAACDSLFAKRDEARDAEANAHRDAIDDVKAEIADVLVGGDDIVTRALAIRTKAE
                     ALDSRELSQSVEQMIRHVVTSNPDAVKGTELDPQAAKNRREKLLAKAEELLPKQPAAA
                     VTADGNPADIAAQLKQAMRSNAFGDLRFSGRDPVEVIDDLRTSWTNAGPLLDDDDRAQ
                     ASKFEDIVKRVLDAAGAAARADRDSAGRGDRDDRGDGEGDRDGRRRRRRADDRGDARR
                     DDRSGDRAGAGRDDGSGDRAGAGRDDRGGDRAGAGRDDRGGDRAGAGRERGGRDGAVS
                     ATPPVGSASADAAATTAAVVSAAAFGAPAAAASLTDPPLPPSKPAPTAVPAAAAAAAA
                     AAAATVAAIPQTAHDAITAPAKIPFEPIPAPEVPSAPLSKPAEQPAATLSTKPAEHPV
                     STKQPEPSFPPVSSQTTQVVEPPRAKTPTMQPPTDALDDAWDMPDDDPTASASADSSA
                     DADAAMQRPEVDQPPSSSEMAGDGAAGGDGIDEPGWD"
     gene            15480..17648
                     /locus_tag="VMZ53_24845"
     CDS             15480..17648
                     /locus_tag="VMZ53_24845"
                     /inference="COORDINATES: protein motif:HMM:NF027993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HUS31763.1"
                     /translation="MRTTTLFAGFVLGASLVTGCIDDGSDKPDQSNVKGGPDGKAEAW
                     GSADNPALFNSSLDYVAANLPRTGEARNIPWAGNYWPVYQDSINHKWAGSTSEAPSTK
                     YGRAFNVTGVENAVSQYHGVDNNSDRTACTTDSQCNSTLGESCAKREGQTSGRCIPTW
                     WGICHAWSPAAILLPEPKHAVTYGGVDFKVQDIKALLTLVHDRTETKFVSLRCDKLDN
                     GTDINFDKYGRPNTGSAECRDTNPGTFHVLMTNYLGKQGESFVYDRTFDSEVWNQPLR
                     GYRITEMTEVSALDANKLVGVTAEGGTTTTKTGNVAQGATTQVGTFPVQAGQLFTLSM
                     TGTGDPDVYVKFGSAPTTSSYDCRPYVEGAAETCTLTVPAGATQAFVAVNGYTASTYS
                     MNIVVGGSIPSSYVFNTAAAKLFKTHMDVDYISESPASQDGNLGATIDRYTHTDNYDY
                     ILEVDAAGKIVGGEWLGASKRKHPDFVWLPIRASGTSVAGGKITYANVKMIYDLSMQD
                     AGGGTTGGGVKTVTDSATVAKAAWKQYGPFNVAAGTTLTATMTGSAGDADLYVRKGAA
                     PTMAAYDCRPYTDGSNESCSIVGPAQVYVGVNGYATSSAYGLTIKYTEGGGTTPPSPP
                     PTTFTHLSQTGSVAQGEMKVFQLAVPANKRLVIRTTSTVDVDLYIQFGAAPTTDAYIA
                     RGYTTSGNETISYTSSSNGTLFIGVHGYAAGAFSVRTADQ"
     gene            17690..18310
                     /locus_tag="VMZ53_24850"
     CDS             17690..18310
                     /locus_tag="VMZ53_24850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS31764.1"
                     /translation="MRAFVVFGLALAGCSFQHGSLSRDDAPVSGDDDAPMIDAAIDAR
                     PDARPDATVTPTCSPTGLVCLAGIASAMTCNGACWVKCTIDGTSGTPVTEATAGQLCA
                     NWGGKLAPIRNAMDQACVATTLFPGQASWIGFEQAPATTTVAGGWSWNGDNVTPTYTN
                     WDTAGNQPDDQNGSENGQEQCAFMPTTGLWHDTDCFGTLYRFSCRK"
     gene            18329..18577
                     /locus_tag="VMZ53_24855"
     CDS             18329..18577
                     /locus_tag="VMZ53_24855"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HUS31765.1"
                     /translation="MPTPEVIDRVRALHDKLEAEPHAALEPVRPQLRTIVGEPHDAAH
                     YSSLSERLQAAYEALEVEHPTLAAAVQATMNALNAAGL"
     gene            complement(18574..20544)
                     /locus_tag="VMZ53_24860"
     CDS             complement(18574..20544)
                     /locus_tag="VMZ53_24860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HUS31766.1"
                     /translation="MPAPGDRPRDQPWIFRTYAGHSSARASNELYRKNLAAGQTGLSI
                     AFDLPTQCGYDADHPLARPEVGKVGVPIASLDDMHALFDGIPLEQMNTSMTINGTAMW
                     LLALYVALARERGVPEAGLRGTTQNDIVKEYLARGTYIFPPEPSLDLIAETYEYCVAH
                     LPQWNPSNICSYHLQEAGATPVQELAFALADAVGILDRVKARGRVDEKSFSSCVGRVS
                     FFVNAGMRFIEEMCKMRAFGQLWDELCRERYGVTDAKHRQFRYGVQVNSLGLTEQQPE
                     NNAWRILIEALGVTLSRDARCRALQLPTWNEALSLPRPWDQQWSLRLQQILAYETDLL
                     EYGDIFTGNPVIEAKVASLCEAAKRELLEIDGMGGIRGAIETGYCKRELVRSMATRMA
                     RIEKGEQVVVGVNKWTDGLPSPLTAGADGGVFHVDPAAVEAAFASLEQTRKKRDAGRV
                     RDVLARLEAAARAGEPLMELSIECALARVTTGEWAGTLRGVWGEYRAQTGVQGARIAD
                     GDGWTALRQRVEAHVAKTGRRPRLLVGKPGLDGHSNGAEVIAVAAKDAGFEVIYAGIR
                     LEPAAIAQTAVQEDVDVVGLSVLSGSHVELAKAVLEELRARGGSDVSVVLGGTVPQSD
                     HGVLRDLGVRRIFTPSDFRLVDVVGHLLDLCP"
     gene            complement(20556..21500)
                     /locus_tag="VMZ53_24865"
     CDS             complement(20556..21500)
                     /locus_tag="VMZ53_24865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31767.1"
                     /translation="MLRNALVLLAASGCDYAVGLQHIVPERGACGPYASVTPVSIVGV
                     EEARGFSVNADGTMALVTGKDSAGQTRPIVLHLNGSDWEPDPTPNIQTGLTAGMKGAK
                     LAPPEPAPNGAKYPAGPLQPAMLLSTVPTGQNKLQVGRYYWTGTTWQQDTNQPAFSLA
                     GYDVYPGNVWIRVGDANPDVDRVRHTVLWMVTQDPSEGPNKVAIMANTPNSFTLEDRA
                     RTVGFNSLGARVGQAVMTEDRAKLVYSALSGIRSDIFAVAQDTESRTFDDEGGGISTV
                     NTNDDEVEPWINADCSQLYFRRIPAETPNEAGQIFVAQ"
     gene            complement(21513..22373)
                     /locus_tag="VMZ53_24870"
     CDS             complement(21513..22373)
                     /locus_tag="VMZ53_24870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HUS31768.1"
                     /translation="MPRIVAAYVACALIWGTTWYAIRVCIAPDGYPTLLSVAMRFAIA
                     ALLLVPLAIRANAWPKGRVWWWLVAAGVLDAASYSLVYLGEEHIPGGLAAVVYGTQPL
                     ILAIMMTALKISGLTRRHVIGAVISLAGVGVLFLDRLDVSRAQAIGVLLVLGSVVVAT
                     SYSVIMKVRGQGVHGLVATTIFLSVTAVVLGIVALVAREPIRWPPATAPTVALLYLAV
                     VGTVVAFLVYFWLLGRTTLLVTSTLVFVFPLVALLTDALFERAISLGPRAYIGAGITL
                     AGLAVSLRRR"
     gene            complement(22395..23435)
                     /locus_tag="VMZ53_24875"
     CDS             complement(22395..23435)
                     /locus_tag="VMZ53_24875"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HUS31769.1"
                     /translation="MAVLLTACGGGGKKPAAKPDPAAAAVASNAEQATAPQAKAEEPP
                     PMSMAQRMEKTTRAMFIAWNAHEAETIANQYEADAILRIYPMPERTSREAIKAQAANT
                     FVGYPDFKTGVRRAIMKDNIAVVEWTVTGTNTGDTPGTNATKRSVGVHGVSVLQFSDA
                     GLIKEEHRYWDMPTQDAQLDPKAKKGTFRPVAELPTEKPATVASTGSPDEAKNLEVVN
                     AVYAAIDAHKADEVLKYMDPNGGGDDYTEPASRQGTKGIKEYLGVFFKAVPDLKQDKI
                     VQLAAGDLVVSEVNLHGTLKGNLGPMKATNKPINLHFVDIAQVKDGKVVHMTSYANSL
                     ELMPPPAAPQKK"
BASE COUNT         3960 a         7915 c         7675 g         4024 t
ORIGIN      
        1 cgctacgtag cgctcgtccg ggacgagctt ccagaacacg tcctggatcg atttcgggcg
       61 atagcagtcc cagatcagca gccgccgatc gctcttgcgt aggaacgccg ctgctttcac
      121 gagacgcgca gcgaccgagc gccgcagctt gcaatgcgcg accggataga cggccttgcc
      181 cgtgaagttg tcgtcagtcg cgtagcggat gtcgagcacc gcatcgggaa tgagcgtcgg
      241 gacgtcgacg aagtccgccg gcttctcgcg cggcgccgcc gaggcgtgga cgcaccccgc
      301 gatcgctacc gcggccacca gccagacgcg caccatgcct cgatcgtagt atgacgccag
      361 cgcatgtcca ccgatgctcg caacgatggg ctcatcgacg ccttcctgtt cgatgggcat
      421 ggcggcgcgc gggtcctcga ctgggacggc atcgacgcct ggcgcgcgga cgccggcatg
      481 ctctggctcc acgtcgacta tgcgaatccc gcggtcagac agtggctcga cacgaagtcg
      541 ggcatcgatg acgtcatcct ctccgcgcta accgatccgg atccgcgccc gcgtgcgatc
      601 gcgtacggcg acaagctgca gctcatcgtg cgcggcatga acaacaacgt cggctcggcg
      661 ccggagaaca tgatctccgt tcgcgcatgg gtagagcctc gccgtgtcat cacgatgcgg
      721 catcgcgcgt cgacgtcggt gaagcagctc gtcagcgagc tcaagcaagg cgaagggcct
      781 cgcaacgccg cggatctcac cgtgcagctc gccgagcgca tcgtcgatca cgtcgttgca
      841 cgcgtggacc ggctcggcga cgcggtcgcg gcgtgcgaag accaagtgct cgggggcgac
      901 gccggcgatc tgcgcgccac gctcgccgat catcgacgca acgcgatcgc gcttcgccgc
      961 tatctcggcc cgcagcgaga cgcactcacc aagctcgcgt cgatcaacgt gccctggctc
     1021 gagccgaacc atcgcgcacg tctcgccgaa gtcgccgacc agctcacgcg caccgtcgaa
     1081 gagctcgatg ccgcccgcga tcgcgcagcg gtcacgcaag aggagctcgg ctcgcgcgtt
     1141 gcggaagcga cgaacaagcg cctctacgtg ctctcgatct tcacgtcggt gttcttgccg
     1201 ctcggcttcg tgtgctcgct actcggcgtg aatgtcggcg gcgtgccgct gcagaagagc
     1261 gactgggcgt tctgggcgct gtgcggcgtg ttcgcgctct acatctacgt gcagctgtgg
     1321 ctgttcaaga agcgcggctg gctctagcta gaagaacgaa cagtcgatcg cgcgcgtcga
     1381 gacgccgctg gcgcctgcga gacctggcga ggttcctcca cttccgccgg tcgcgcccat
     1441 cgcggtcgtc tgcgggattt ggatcgacgc ggtgccgagg cagacgagtg ccgctgatgg
     1501 accgccacct ccgccgccgc cgtggccacc gccgccgccc gctccgccga gcccgccgcg
     1561 cgcgccgcag ccgccgtcgt cttgctcgct gctgttgctg tacacgccgc cgagaccgta
     1621 gccaccgccc gtgccgccgg agccgccctg acctccgcgg ccgccatcgc cgccgcgatt
     1681 cgcgatgacc atcgacgact tgatcgcgac gctcgaatcg acggcgatca cgccgaacga
     1741 tccgccgccg ccggtgccgg caaagccgcg tccgccgccg caaccaccac cgccgccgcc
     1801 gccgcccgac gagccggacg aatcgcagct cgcggtcccg ccaccaccgc cgccaccacc
     1861 gccaccaccg tagccagcgg ttccgttgag accatcgccg ccacccgacg ggatgtacat
     1921 gacgccattg aacgtgccga tatccattcc ggccgcgccg ttgctgcctg cgctaccagg
     1981 aaagccgtct gcgccgactt ggccatcgcc tgcgccgccg catggtgggt tgtgaccgcc
     2041 accaccgccg ccgaaaccgc cggactgcgc aaggcccgcc gagtccccac ccgtgttgcc
     2101 tgcgctgtca ccgacgcccg cagcgccgcc caatccgccg gtgcgtccgc acgacgacgc
     2161 gcccgaagtt ccgaccgctg gaagcgcgtg gacgtcgcaa ccgaaccccg tggagtactc
     2221 cgcgccggga tttccattgc cgccggcacc gcccatcgca ccgacgctgc cgttgccgcc
     2281 gtcattgccc ttcgcgccga tgcccggcgt gatcgtgacg tcgcgcagct cgatcagctt
     2341 cgagctcgtg atcaccactg cgtacgagct cgctccagcg ccggtcgcat cggtgctctt
     2401 gatcgtgacg ccgtcgatgg ccgcgcctgt ctggatttgg tcgaacgtga ctgcaggcga
     2461 cgcagcgttg atctcggtga cgaccgccgg tgtccgcgtc cacgtcgcgt cgaatccgcc
     2521 gtacacggcg acgcccgcct tcatcgcgac ttgctcggga tacgtaccac cttcgacaaa
     2581 caccgcgagc ggcggaacgg tgttgagcgc cttcgtgatc gcagcgttga tcgtcttcat
     2641 cggcgccatc ttggtgcccg ccgacgagtc gttgcccatg cgcgatacgt agaccgcgtt
     2701 gcacggcagg ttcccacagc tcgccgcatc gatcatcggc gcgctcgagt cgccgctgct
     2761 cgtaccgcta tcgacggtac tgccgttgcc cgacgcacat gcggcaactg cgagccacag
     2821 tagcgcgctc ctcattgagg aaagtgtagc tgacgccgtg tgccgttacc tgcgaaacaa
     2881 gtcacgcgcg gcgcgctgcg tcgatcgtgg gcggatcgcc actgacgcca tgcagataca
     2941 tcgcttgcga gccgtatgcg atgagcttgc cagcgtcgtc ccagacttcc gcatcggcga
     3001 tcgcccaacc accgagtgcg cgacgcatcg tgaccgcgac gaggagccac tcacgcatcg
     3061 tgtcgtcgat cgtgtaaacg gtcaggtcga ggctaggtgc gtagaatcga tagcctccgg
     3121 ggccgatcgc gcgatgcagt gcagttggca tcgtatcgat gagcggcgga atcgcgagtc
     3181 gatcgagacg gccctcggca tctcgctgag gcgcgcgata gcgaaaccag cgggcgtaac
     3241 gcgcggggcc tgcggcaaag tctggttgcc agaacgctgg accgtcagcg agcttgcact
     3301 cgagctggtg atagaagcgg aaccgggtgt gcggattgtt ggacgcaccg tcgtcgaccg
     3361 ggagcgcatc ggcgaggctt ttcaccgcgg ggaacgggac gcacgcgacg tcgggaccct
     3421 tgcgatcgcg catgaacgtc gcgatgagct cgagccccgg ctcgccgtct ttctcctgcg
     3481 tgtggcgaaa aatcacgcgc gtctgcaccg cgctgttacc gcgccggatc acgtgcacgt
     3541 cggcgaggca ctttccggga tgaatcggcg acgcgaagat cgtcgtggcc gacgcgaggc
     3601 gaaacgtcgg gtcgccgatg tgtgcctgtg ccgcccgcag tgcagccgtc atcacgacgc
     3661 cacctgacgg caacaagtag tcccaatggt cggggagcgt caggtgatag cgaccgggca
     3721 cgctgtcgtc ggcgcgcacc gcggtatcgg cccggagatc agcgaacgac atcgcggcgt
     3781 acgttagcgc gaaacactac gcgagcggct gatcaccgac ggcgacgtgg tcgtggtagg
     3841 cgagcagcgg ccggatctcg tcgagcaccg acgccggtcc cgtcgtctcg agcagcttgc
     3901 agaccgaggc tttcttgctc gctagctcgt agtgactcac ccaatgcagg agctcgccat
     3961 gttgctcggc gctctcgtac ggaaaccgga cacgtggttc cttcatgccg atgcgccacg
     4021 gcttcggcgt gagccgcgcg cgaaaggatg cctgcgcctt gcagagctgg atgtacagct
     4081 tgtcggtgcc aacctcttcg agcagcgcga tcgcttctgc gctcttcgga tcgtacgtgt
     4141 gattggtgac cagcaagcgc aagcctgcat gcgtgcggta cacgcgcacg gcgagctcgg
     4201 gatgcgacgc ggtccactgc tgcacgcgtt ggatcgccgc gccctcgccc gcaccgtcgc
     4261 cgaagtcgac gtcgatgaac atcgcgcgtt cagcgttgag gaccagcgcg ccgtacaagt
     4321 tgcgtgtgat gacgacgccg ttgtcgagac gctgcacgat ctcttcacgc agcgggcgat
     4381 caccgtagga atacctatcg aggcggcggc ccgacgcgaa cagcgccgcg agctccgccg
     4441 cgcgttggtt tgctttctgg cgcgcgtcgt cttcgctgac atcagaccat ctccagcacg
     4501 acgcactcga actgccggag gttgcttgtc cacgtgccca gtacttcggg aaatgcacgt
     4561 cgacaaaata gccgtcgacg gagatctgtg ccagaggctt aggccggaac ggcgaggcga
     4621 agcgcgcgcg cgagccgctc gaggtctgac acagcggtca gcgcattgat gtcgccgctc
     4681 ttgccgcgtg cgcgcagctc cggcagacgt tggagcaagc gccacgttgc gttttgcgcg
     4741 aaccagagat cgacggagct cttcatgcgc gccgcaactt cggccgctcg cgtcactgta
     4801 tcgagcgcgg cgaaatcgat ctcggaatcc ttcgatggcg cgacgcgatc gatgaggcgg
     4861 cgcaacccct cgctcgcggc gagcgcaatt tccggcgtgt cgaggctgac cttcacttcg
     4921 gtcgcctcgg cgatgtgatc gcgcagcgct tggaagctcg cgttttccag cgacaggttc
     4981 gcgagcacgc gctggcgcag tgacgcttca gcgacggtat gcagtgcttc gggcaccggc
     5041 aggtcgtgcg cgacgagcca gcgaatgagc ggcgcgtgct gatcgtaggt gtccacgagc
     5101 gcttcgtgcg cggagccgat cgcttcaccg aggaccgccg cgagcacgcg ctctcgcgag
     5161 ccaggaagca gcgcgccgag cgagagatgt gcaccgggga agtggcgatc gatcgcgcgc
     5221 tgggcggcaa atacgtcgcc ggtctctagc gagtcgcgca gctccttgag catatgcgcc
     5281 cattccgatt cgctcggcgg gacacgaacg ccgccggtga cgtgctgctc gccgaggtgc
     5341 aagccggcaa agcacagcgc ggtcgcggcg tcggtcagcg atgacttcac gcagacggtg
     5401 cccgcaacca tccgcgcgcg gccgctcttg cgctcgacgc gatcgagaaa ctcgacgtgg
     5461 aagccgtcga ccgccaccgc gcgcgtcgga tccggctcga cgagcgcatg cacagcgacg
     5521 tgcgcagcca ccttgctcgg atcgacccgc gctggacgca cgaacatcgt ccacacgcgg
     5581 cggccatcgc cgtgatccgg aacgttcgag cgcgcgagcg agagtctctc gacgagctcg
     5641 ctctcgaccg atacgccact gatcgaattg agcagctcgg cgacgcgcgc cgcgtactga
     5701 atgcactgca cggtctcgat gcccgacaag tcgtcgaaga accagccgca gctcgtgtac
     5761 atgagcatct cgtgacgagc catctccatc agcgagagag cgcgcacgcg ttcctccgtc
     5821 gacagcgagt gcgaggcatg cgtctcgagg aaacggtcgc ggccttgccg gccgttcagc
     5881 agcgcatcga catacgcgtc gcgcgcgacc cacggatcct tgaacagcgc gcggccgaca
     5941 gaatcgagcg cgcccgatgc ttgctcgcgc agccaatcaa acgcatcgcg cagcggacgt
     6001 cgccacttct ggttccagcc cggcttgccg ccgctgttgc agccgcagtc gttgcgccag
     6061 cgctcgatgc catggatgca gctccagctc gaattctcga gcagctggac ttcccacgtc
     6121 gccgggaact tcgtgcggaa ctcggcgtag ttcgtgaggc gcgtcccgtt ccagccctgc
     6181 tctacttgcg acagcgccca cgcgagcgcc atgtcgccgt agcgatgatg gtggccgtac
     6241 gtctcgccgt cggtcgcgat gtgacacagc gtcggcccat caccttcgac ggggccgcga
     6301 ttcgtcatac gcgagatgat gtgatgaccg tcggagagga ggcgctcgaa cgcgaccgct
     6361 tgcgacgtcg cgccgtcgta gaagaacaga tcgatcgagc ggcccgacgg cagcttgtac
     6421 ttgtagacgc ggcccggatc gacgggcgtc gtgcgccact cgccgccggg cggtcgccac
     6481 gccttggcct gatgtggcgc gagcaccgtg aacaagatgc cctcggcagc aagcgcctcg
     6541 agcgaggccg tatcgacggc gcactcggga agccacatgc cttcgggcgc gcgatgaaac
     6601 cgccgcttga agtcggcgat gccccaccgc acctgcgtga ctttgtctcg cggcgaggcg
     6661 agcggcatga tgatgtggtt gtaggcctgc gccattgcag agccatgacc gctgaacctc
     6721 tcgcggctcg cgcggtcggc agcgaccagc gattcgtgga catctttcgc gtgacgctcg
     6781 agccacgaca tgagcgtcgg cccgacgttg aagctcatgc gctcgtagtt gtcgacgatc
     6841 tggatgatgt ggccttggcc atcgatgacg cgcgccgcgg tgttgggccg atagcactcg
     6901 gcggtgatgc gctcgttcca gtcgcgatac ggatgcgcgc tcggctgcgg ctcgatggct
     6961 tcgagccacg ggttctcgcg cggcggctga tagaagtggc cgtggaagac gacccacttc
     7021 gaagacttct cgctcactcg ggtacaaata ctacgatgcc cagcggcggc acggtgacga
     7081 ccagcgactg atcgtagccg tgccaaccct gcccatccgt atgcatcccg ccgcggttac
     7141 cgacgccgct gccgccgtag atcgaggcat cgctgttgag aatttctcgc cagaagcctc
     7201 ccttgggcac acccaagcga tagccctcgc gggggacggg tgtgaagttc gcgacgacga
     7261 gcgcaggcgg atcgccaccg ttgccggtgc gcagatacgc gatgatgctc gagccactgt
     7321 cgtcgccgac gagccagcgg aagccgacgg gatcgcaatc gccgacgtgc agcgacttgt
     7381 atttccgata ggcgctattc agatcgccga cccaacgcgc gatgccggcg tgcgcgggat
     7441 gcatgccgca gccccagtcg agctcttggt cgtggttcca ctcgttccac acgccgatct
     7501 cgctgcccat gaacagcagc ttcttgcccg gcgtcgtcca ctgatagcca tagagcaggc
     7561 gcgcgctcgc gtacttctgc cacgggtcgc ccggcatctt gctgaggatc gagcccttgc
     7621 cgtgcacgac ttcgtcgtgt gagagcggca ggcagtagtt ctcgctgttc tgatagatcg
     7681 cgcgaaacgt gatctcgccg tggtgccact ggcgatggat gggctcgcgc tcgaggtact
     7741 tgagcgtgtc gtgcatccag ccgaggtccc acttgtagcc aaagcctagg ccgccgtact
     7801 cgggcggacg cgacacgcca ccccatgccg tcgattcctc ggcgatcgtc tgcacgtcgg
     7861 ggaacatcgt gtagacggcc ttgttgaagt cctgcaggaa gtggatcgca tcgcgatcgt
     7921 gattgctgcc gtcttcgtta ggcacccact cgcccgcttg acgcgagtaa tcgcgataga
     7981 gcatcgacgc aacgccgtcc acgcgtaggc catcgatgtg atagcggtcg agccagttga
     8041 tcgcgctcga gatcaggaac gagcgcacct cgtgccgcgc gtagttgaag atgtagctgc
     8101 gccaatcggg gtgaaagccg cgccgcggat ccgcatgctc gaagagatgc gtgccgtcga
     8161 agtagccaag gccgtgctgg tccgtcggga agtgcgcggg cacccagtcg aggatcacgc
     8221 cgatgccgcg ctggtgcagc tcgtcgacga agcacatgaa gtcttccggc gcgccgtagc
     8281 gcgtggtcgg tgcgaagtaa cccgtcacct gatagcccca cgatccgtag aacgggtgct
     8341 ccatgaccgg catcagctcg acgtgcgtga agccgagctg cgtgacgtga tgtgcgatct
     8401 gctggccgag ctcgcgatag ccgagcactt caccattgcg tcgccgccat gatccgagat
     8461 gcagctcgta gatgctcatc ggtgcgttgc gcgccgcacg ctcgccgcgc gacttcatcc
     8521 agtcgccgtc cttccactga tgctgcggct tccagacgat cgacgccgtc atcggcggat
     8581 gctcgtagcg cgcggcgaat ggatccgcct tctcgtgcgt cgcgccatcg gggccgacga
     8641 tcgcgtactt gtagcgagtg ccgtgaccaa cgcccttcag cacgccttcc cagattccgg
     8701 acgactcgcg cgggcgcagc ttgtcgctgc catgcaccca gccgttccaa tcgccgatca
     8761 cgctcacgct gcgcgcgctc ggtgcccaca ccgcaaagaa cgtcccgcct tcgacgggat
     8821 gcgcgccgag gtgctcgtag agcttgaagt gcctgccctg gttgaacagg aacaagtcgt
     8881 cagacgtgag cagggactgc gccgcgggtt tctggaaggg ctgcgtcacg gcggcactct
     8941 actgcgctag cgcgggagta caaggaagcc aacctcgcct aactaggcgg tcacgttgcc
     9001 gaaagcaaca gtacgcaacc gccgcaacgt cctgccgata aggggccgca aggaggcaga
     9061 caagacaatg cgcaagatga tgatggtggc cctgttctcg gttctctcgc tctcggcgtg
     9121 tgcggtggag gagccggtgc aaaccgctca ggacgaggcg cccgccgaac agcaaccctg
     9181 caacaccgcg gacgatcacc acgctcgccc gaccaacgct cgcgagctcg aggaggaagg
     9241 cgaagccgtg tggctccagt gcgacgacaa ctccgagtgc gagagcaacg tgtgcgatct
     9301 cgagatccac acctgccgcg agtatccgtt ctgatcgcgg cgcaccgcga atgaagaagc
     9361 ccggcccgca aggtcgggct ttcttcgttt cggtcgtccg aaattcgtac gcaaccgccg
     9421 cgacgacctg ccgataagga gccgcaccaa gggaggacgg aaacgatgcg caagatgatg
     9481 ctggttgcga gtctgatgag tgcggcatgt ggctcggtcg aagagcccgc tgtgatgaac
     9541 gatgacggca cgggttccga cgtcggctcg ggctccgggt cgggctcggg ctcgggctcg
     9601 gcgtcaccgg acgcaggtgt gccggtcgac acgggctgca tgaaggccgc cgactgcggc
     9661 gcgaacatgg cgtgcgatat cgcgacgcat gcatgcgtgg caggcgcgct gacgatcgat
     9721 ccgtcggact tcgtgatcga cagcggcgtg tggtggacga cgacgagcgg cccggttctg
     9781 cacggcacgt ttcaaggccc gacgagcgcg gtcgtcgaag tgaagaccgg tgcgtcgcag
     9841 ccggtgatgg cgacgctcac cggcaacgcg tgggcagtaa cgcttcccgc cggcacgatc
     9901 tcgagctcgt acgctccgat cgttgtcatg ctgacggatc cgagcggcag cgacatcgag
     9961 ctcgcgcgca cgcttggcct cgatagcgcg ccacccgcga tcaccgtgca aggtagcatc
    10021 cgcgacgagc gcaacgacac gatcgacttc gcaaccggcg agcccgttca cacgcacgcg
    10081 ggcgcggcga cggacctcgc ctcgacaggc tgcgcgagcg tctacaagta cgcctacctc
    10141 atggacgcgt cgcccgcgtt cggttcggcg acgtcgccga acgacctcgc gtggaagctg
    10201 cgcatcaccg acgcgaagct gcagacgggc tcgcaacagt tccgcgtgcg caccgacgca
    10261 ggcgcagtgc tgatcaattg gagcgcgctg ccaacacccg acgcggcagg tacgtcgacg
    10321 gtcaagctca cgcgcagcgg tggtgcgtac gcgatcgccg cactcggcac gcgcacgggc
    10381 cagttcttca tcgatgttcg cgcgacggac tggggcggcc tctcgtcgac ggcgagcttc
    10441 tgcttcgaca accatccgct cgccgcaccg ctctcgatct ccgcgatcga gaagggcgac
    10501 ctcttcggac tgtcgacgac gaacgacgcc ggtatcgccg cgctgttcgc ggacaacgcc
    10561 gcggtgaacg tcgtcaagca gcacatcgtg cagcaggtcg ccgagccgat cagcttcacg
    10621 tggtcgctcc aagcgacatc accgtcgtac accgcgcagc aagtctgggg ctacgtcgaa
    10681 gagacgttca acggcggcta tatgtgccag ccgggcaact cgaagccgga gtgcgtgatg
    10741 acgacgaagc cgagctcgtc gtatccaccg gcgagcgcgt ccccgtcggg cggcactctc
    10801 gcaagcagct actccgtcgc gcttcgcgac gacgtcacgg gccagatcgt cgcaacgagc
    10861 acgacgaagt cgatcacgtg gacgatcccg gcgcgtggct cgagcgaagc gccgcacggc
    10921 tacaccgtcg aggtcgcgca gtcgggcttc tacgcgattc ggccgctgag cgtgttttgg
    10981 tacggctatt ggaccgactc gcccgtcggc gtcagcacgc tgatgggctc gacgtacacg
    11041 ggcacggcgc cctacgacat caagggcacg cagtgcgtgc agatgattca aaccgcgttc
    11101 ggtccgatgt gccagaacgc gatcaagtac tggggcatga gcgctatcga ccaggtgacg
    11161 ctcagctaca gcccgttcgt cgcgaccttc tcgacgggcg caacggcgac gcagctgtcg
    11221 gccccgagct atgtgatgcc gagcatgctg acgtcgcaag cgttcacgtg ggacagcggc
    11281 gacgacgatc tgcccggcgc gctgtaatcg cgcctccctg catcccccgc tgtgcccacg
    11341 tgggcacgcc gggcacgctg cactcgcgcg cacactcacc tcgcccgctc gacctcgagg
    11401 caaacgtctc gaggtcgtcg cgcgtttgat gccccgcagc gaacgcgcct ctcatttagg
    11461 ggtctcttcg cgaaacccat catgacggtg tcatgacgtt caaatccgcg gagttaaccg
    11521 tccgtacatc gtcatgacgg gatccggttg gagaccgatg tagtccggga tctgcgggcg
    11581 cggccgtgct cgtgaccgat tcgatgaccg acttcgatca ccgacttcga tcaccgactt
    11641 cgatgatcga ctacatggat caccgagatc gatcaccggt gtgacgccaa cgccgaaggg
    11701 cgccgcggcg cggggcgtcg atcagtccca gccgggctcg tcgatgccgt cgccaccggc
    11761 cgcaccgtcg cccgccatct ccgaagagga cggcggctga tcaacctcgg ggcgctgcat
    11821 cgcggcatcg gcgtcggccg acgaatcggc ggacgcagat gccgtcggat cgtcgtcggg
    11881 catgtcccac gcgtcgtcga gcgcatccgt cggcggctgc atcgtcggcg tcttggcgcg
    11941 cggcggctcg acgacctgcg tcgtctgcga cgacaccggc gggaacgacg gctcgggctg
    12001 cttcgtcgag accgggtgct cggccggctt cgtcgagagc gtcgcggccg gctgctcggc
    12061 cggcttcgac agcggcgccg acggaacctc gggcgccgga atcggctcga acgggatctt
    12121 cgccggagcg gtgatcgcgt cgtgcgcggt ctgcgggatc gcagcgactg tcgcagcggc
    12181 tgccgcagcc gctgcagccg ctgcagcggg aaccgcggtc ggtgccggct tcgacggcgg
    12241 cagcggcggg tcggtcagcg acgccgcggc agccggtgca ccgaacgcag ccgcgctcac
    12301 gacagccgcg gttgtcgcag ccgcgtcagc ggacgcagaa ccaacgggtg gcgttgcgga
    12361 caccgcgccg tcgcgaccgc cacgctcgcg acctgcgcct gcgcgatcac caccgcgatc
    12421 atcacgacct gcgcccgcgc gatcaccacc gcggtcatcg cgacctgcac ctgcgcgatc
    12481 accactgccg tcatcgcgac ctgcgcctgc gcgatcacca ctgcggtcat cgcgacgggc
    12541 atcaccgcga tcgtcagcgc ggcggcgccg gcggcggcca tcgcgatcgc cttcgccgtc
    12601 acctcggtca tcgcgatcgc cgcgaccggc agagtcgcgg tctgcgcgag ccgcggcgcc
    12661 cgcggcgtcg agcacacgct tcacgatgtc ctcgaacttc gacgcctgcg cgcggtcgtc
    12721 atcgtcgagc agtgggcctg cgttggtcca cgatgtgcgc agatcgtcga ttacctcgac
    12781 cgggtcgcgg ccggagaagc gcaggtcgcc gaacgcgttc gagcgcatcg cttgcttgag
    12841 ttgcgcggcg atgtcggcgg ggttgccgtc ggcggtgact gcggcggcgg gctgcttggg
    12901 gagtagctct tcggccttcg cgagcagctt ctcgcggcga ttcttcgccg cttgcggatc
    12961 gagctccgtg cctttgacgg cgtcgggatt cgacgtgacg acgtggcgga tcatctgctc
    13021 gacggattgc gacagctcgc ggctgtcgag cgcctcggct ttcgtgcgaa tggcgagagc
    13081 ccgcgtgacg atgtcgtcgc cgccgacgag gacgtcggcg atttccgcct tcacgtcgtc
    13141 gatcgcgtca cggtgggcgt tggcttcggc gtctcgcgct tcgtcgcgct ttgcgaacag
    13201 cgaatcgcat gcggcgcgga aggctttgta gacggcgtct gcatcgcggc gcgggacgaa
    13261 gccgatttcc ttccactgct gctggaggtt cttgatttcg gcgatcgcct tgccccagcc
    13321 accttcgccc ggcgcgttgt tcgcgaccgt ttgggcgcgc gcgatcaggg cttgtttggc
    13381 ggagaggttc tgcgcctctt cggcgttgcg cgcgtcgagc tgcggcttgc ggcgctcgaa
    13441 gaagcgatcg cacgcggccc ggaatcgctt ccacaggtcg tcaccttgct tgcgcgggag
    13501 gtggccggac tgcttccagg cggcttgcag ctctttgagc cggttcgcgg ttgcttgcca
    13561 gtcggtagac tcggcgagcg attccgcttc ggcgatcagc gcttccttcg ccttcgttac
    13621 gtcggcgaac ttctccgact cgacggcgcg gaagcctttc accttgtcgt agatctgatc
    13681 gcagagcgac ttgaatgatt cccacagctc tttgctgcgg cgctgcggca tcgggccgac
    13741 ttctttccag agcgcttgca gctggcgaag gcggttgccg agatcgggag acgactcctc
    13801 ggcgagcatg tctttggccg tggcgatcag cgcttcggcc ttcggcacgt tcgcgaaccg
    13861 ctcccagtcc tctgcttcac gaagctcgct cgcacgaacg acgagcttgc cgcgcgcctt
    13921 cgtgtagcga tcgctcagcg attcgcgttg gtcggcgggc accttgccga tttgctcgaa
    13981 cgatcggccg gcttgttgga gcaggcggtc gatgccacgc gtgtccctgt cggaaccggc
    14041 ggcgagcttc tccatatcgt cgagcatcgc gctgagcgac gtcgcgattg cagcgccgcg
    14101 ctcggcgtct tctttgcggc gcgcttcgcg agcggcttgc tcttcggcgc gacgtgcatc
    14161 ttcctcggcg cggcgcttct ggcgctcttc acgctcggcg tcggcggcgg ccgggtcggg
    14221 cttcgcctcg gtggattcgg gcgacttcgc ctgtgcctcg cgagccgcgc gttcggctgc
    14281 ggcacgctct ctctcgcgct gcgcttcacg ctcgacggct cgcagctcgt cggcggaccg
    14341 cgcttgttgg tcgtgcagct ccttgcgctt ccagaagcgt tcgaccgcct tcgtgaagcg
    14401 ttcgtcaccc tcgtcggcgc cttcgagctt cgaccacgat tcttgtgcgg ccttgacgac
    14461 gccttcggac ttcgcgaaat cgaatgtgtc ggcgaggcct tcgagcgtgc gcacgagctg
    14521 cgccttctcg gcgcggcgac gcttgacgtc gtccggaacg ctcggctttt gcttcgcctt
    14581 ctccgacgcg gtgatctcct gcacgatctt gcgagcgcgc tggcggaccg ccttgttctt
    14641 cgccttgttg gcgacgttct cgaggcgttc gacatcgtcg agcttctcga cggcagcgag
    14701 gccggtctct ttgatcgtcg tgtcgatcgc gatcgcgcga agcgtctcgc catcatcgat
    14761 gcgagcgatc gcagcggcgc ggatatcttg aggagcgtcg gaatccttcg cgagctcggc
    14821 gagcgccttg ggatcgcgca gctcttcgaa cgccttcttg cgaattccgg atagcttcgc
    14881 gtgaacggct acgtcgatga gcgcgtgctg gtcacccagc ttcgtgatcc ccgagagcgc
    14941 attcgatgcg gcgtcctcat tctccttgca ggcagtcgac gcgtagagct gagccgcacg
    15001 ttcacccgcg aattcacgca ggctccgttc ggtctcgtct gcggcgatct ccgcgaggac
    15061 aggtgcttcg tcgatcttct cgatcgcgat acggcgcacg ccgatatcac ggtcggttcg
    15121 agcaacttga tgaaggatgg cagcgtcgtc cgaggtcaaa gccctgacgg cttcggtgcg
    15181 aacgcggacg tcgctgtggc ggtatttggg gcggaagaga tctgcgatcc ccataggcag
    15241 gggcgggaat ctaagcgcct atctaggggg ctgcaatacg ctttatacgg ttcatcgcag
    15301 tcgcgaaacg agacgtggga gtcgcgacct ggctagcggg ctagccagtg aaccgttgtc
    15361 ctcggcagcg ttggggattt gacacgcaga ttccttgcga ccttgcgtgt ggtcagggct
    15421 ctccccgacg gcggcacgct gcgtgctcat ggccgcgccg cataaaggag agactacgaa
    15481 tgcgtacgac gacactgttt gccggcttcg tgctgggcgc gagcctggtc accggctgta
    15541 tcgatgatgg cagcgacaag cctgatcaga gcaatgtgaa gggcggtccg gacggcaagg
    15601 cggaagcctg gggctccgcg gacaaccctg ccctcttcaa ctcttccctc gactacgtcg
    15661 ccgcgaacct gccccgcacg ggcgaggcgc ggaacatccc gtgggcgggc aactactggc
    15721 ctgtctacca agacagcatc aaccacaagt gggccggctc gacgagtgag gctccctcga
    15781 ccaagtacgg ccgcgcgttc aacgtgaccg gcgtcgagaa tgcggtctcg cagtaccacg
    15841 gtgtcgacaa caacagcgat cgcaccgcgt gcacgacgga ctcgcagtgc aacagcacgc
    15901 tcggcgaatc gtgcgcgaag cgcgaaggcc agacgagcgg ccgctgcatc ccgacgtggt
    15961 ggggcatctg ccacgcgtgg tcgcccgccg cgatcctcct tcccgagccc aagcacgccg
    16021 tcacctatgg tggcgtcgac ttcaaggtgc aggacatcaa ggcgctgctc acgctcgtgc
    16081 acgatcgcac ggagacgaaa tttgtctcac tccgctgcga caagctcgac aacggcaccg
    16141 acatcaactt cgacaagtac ggccgcccga acacgggcag cgccgagtgc cgcgacacga
    16201 accccggcac gttccacgtc ctcatgacga actacctcgg caagcaaggt gagtcgttcg
    16261 tctatgaccg cacgttcgac agcgaagtct ggaaccagcc gctgcgcggc taccgcatca
    16321 ccgagatgac cgaagtctcg gcgctcgatg cgaacaagct cgtcggcgtg accgccgagg
    16381 gtggcaccac gacgacgaag accggcaacg ttgcgcaggg cgcgacgacg caggtcggta
    16441 cgttcccggt gcaagcgggc cagctcttca cgctcagcat gaccggcacc ggcgacccgg
    16501 acgtctacgt caagttcggc tccgcgccga cgacgagctc gtacgattgc cgcccgtacg
    16561 tcgagggcgc ggccgagacc tgcacgctca cggttcccgc gggcgcgacg caggcattcg
    16621 tcgccgtcaa cggctacacg gcctcgacct actcgatgaa catcgtcgtc ggtggctcga
    16681 tcccgtcgag ctacgtgttc aacaccgcag ccgcgaagct gttcaagacg cacatggacg
    16741 tcgactacat cagcgagtcg ccggcttcgc aggacggcaa cctcggcgcg acgatcgatc
    16801 gctacacgca cacggacaac tacgactaca tcctcgaggt cgatgcggcc ggcaagatcg
    16861 tcggcggtga gtggctcggc gcgagcaagc gcaagcaccc cgacttcgtg tggctgccga
    16921 tccgcgcgag tggcacgagc gtcgccggcg gcaagatcac ctacgcaaac gtgaagatga
    16981 tctacgacct gtcgatgcaa gacgcaggcg gcggcaccac gggtggcggc gtcaagacgg
    17041 tcaccgattc ggcgacggtc gcgaaagcgg cgtggaagca gtacggcccg ttcaacgtgg
    17101 cagcgggcac gacgctcacg gcgacgatga ccggctcggc gggagacgcc gacctctacg
    17161 tccgcaaggg cgcggcgcca acgatggcgg cttacgactg ccgcccgtac accgacggct
    17221 cgaacgagtc ctgctcgatc gtcggcccgg cacaggtcta cgtcggcgtg aacggctacg
    17281 cgacgtcgag cgcgtacggc ctcacgatca agtacaccga gggtggcggt acgacgcctc
    17341 cttcgccgcc gccgacgacg ttcacgcacc tctcgcagac cggctcggtc gcgcagggcg
    17401 agatgaaggt cttccagctc gcggtcccgg cgaacaagcg cctcgtgatc cggaccacct
    17461 cgacggtcga cgtcgacctg tacatccagt tcggcgccgc gccgacgacg gatgcgtaca
    17521 tcgcccgtgg ctacacgacg tcgggtaacg agacgatctc gtacacgtcg tcgagcaacg
    17581 ggacgctgtt catcggcgtg cacggctacg ccgccggcgc gttctcggtt cgtaccgcag
    17641 accagtagag tcctcgttca gcgagcgccc tccgtgcgac aatgcgcatg tgagggcgtt
    17701 cgtcgttttc gggctcgcgc tggcgggctg cagcttccag cacggctcgc tgtcgcgcga
    17761 tgacgcgccg gtcagcggcg atgacgatgc accgatgatc gacgcggcga tcgacgcccg
    17821 acccgacgca cgccccgacg cgacggtcac gcccacgtgc tcgccgacgg ggctcgtatg
    17881 tttagcgggc attgcatccg cgatgacgtg caacggcgcg tgctgggtga agtgcaccat
    17941 cgatggtacg tcgggaacac ccgtcaccga ggcaaccgcc gggcagctgt gcgccaactg
    18001 gggcggcaag ctcgcgccga ttcgcaacgc gatggatcaa gcctgcgtcg caacgacgct
    18061 gtttccaggc caggcgagct ggatcggatt cgagcaagct ccggccacca cgaccgtcgc
    18121 aggaggctgg tcgtggaacg gcgacaacgt gacgccgacg tacacgaatt gggataccgc
    18181 cggaaatcaa cccgacgatc agaatggcag cgagaacggc caagaacagt gcgcgttcat
    18241 gccgacgacc gggctgtggc acgacaccga ttgcttcggc acgctgtatc gattcagctg
    18301 ccgcaagtag atctgctacg gtgcccgcat gccgacgccg gaagtgatcg accgagtgcg
    18361 cgcgttgcac gacaagctcg aggcggagcc gcatgctgca ctcgagccag tacggccgca
    18421 gctgcgcacg atcgtcggcg agccgcacga tgcggcgcac tactcgtcgc tgtcggagag
    18481 attgcaggcc gcctacgagg ccctcgaagt cgagcacccg acgctcgcag ctgccgttca
    18541 agcgacgatg aacgcgctca acgccgccgg gctctaagga caaagatcga gcaagtggcc
    18601 gacgacgtcg acgagacgga agtcgctcgg cgtgaagatc cggcgaacgc cgaggtcgcg
    18661 cagcacgccg tgatctgatt gcggcaccgt gccaccgagg acgaccgaga cgtcgctgcc
    18721 gccgcgagcg cgaagctctt cgagcacggc tttcgcgagc tcgacgtggg agcccgagag
    18781 gaccgagagg ccaacgacat cgacgtcttc ttgcacggcc gtctgcgcga tcgctgcggg
    18841 ctcgagtcga atgccggcgt agatgacttc gaagccggcg tccttcgcgg cgacggcgat
    18901 cacttcagcg ccgttactgt ggccgtcgag tccgggcttt ccgacgagga gacgagggcg
    18961 gcggccggtc ttcgcgacat gcgcttcgac gcgttggcgg agcgcggtcc agccatcacc
    19021 gtcggcgatg cgtgcgcctt gcacgcccgt ctgcgcgcgg tactcgcccc acacgccgcg
    19081 cagcgtgccg gcccattcgc cggtcgtgac gcgggcgagc gcgcactcga tcgagagctc
    19141 catgagcggc tcaccggcgc gagcggcagc ttcgaggcga gcgagcacat cgcggacgcg
    19201 gcccgcatcg cgcttcttgc gcgtctgctc gagtgacgcg aacgcggctt cgaccgctgc
    19261 gggatcgacg tgaaacacgc cgccgtctgc gcccgcggtg agcggcgatg gcagaccgtc
    19321 ggtccacttg ttcacgccga cgacgacctg ctcgcctttt tcgatgcgag ccatgcgggt
    19381 cgccatcgag cggacgagct cgcgcttgca gtaaccggtc tcgatcgcgc cgcgaatgcc
    19441 gcccatgccg tcgatctcga ggagctcgcg cttcgcggcc tcgcagagcg acgcgacctt
    19501 cgcttcgatc accggattgc cggtgaagat gtcgccgtat tcgaggaggt ccgtctcgta
    19561 ggcgaggatc tgctgcagcc gaagcgacca ctgttggtcc cacgggcgag gcagcgacag
    19621 tgcttcgttc cacgtcggca gctgcagcgc acggcaccgc gcatcgcgag acagcgtcac
    19681 gccgagcgct tcgatcagga tgcgccacgc gttgttctcg ggctgctgct cggtgagccc
    19741 gagcgagttg acctgcacgc cgtagcggaa ctggcggtgc ttcgcgtccg tcacgccgta
    19801 gcgctcgcgg cagagctcgt cccagagctg gccaaacgcg cgcatcttgc acatctcttc
    19861 gatgaagcgc atgcccgcat tgacgaagaa cgagacgcga ccgacgcacg aggagaacga
    19921 cttctcgtcg acgcggccgc gcgccttcac gcgatcgagg atgccgaccg catcggccag
    19981 cgcgaatgcg agctcttgca ccggcgtcgc gccggcttct tgcaggtggt agctgcagat
    20041 gttcgaggga ttccactgcg gcagatgcgc gacgcagtac tcgtacgtct ccgcgatcag
    20101 atcgagcgac ggctccggcg gaaagatgta cgtgccgcgc gcgaggtact ccttgacgat
    20161 gtcgttctgc gtcgtgccgc gcaggcctgc ttcgggaacg ccgcgttcgc gagccagcgc
    20221 gacgtacagc gcgagcagcc acatcgcggt gccgttgatc gtcatcgacg tgttcatctg
    20281 ctcgagcgga atgccgtcga acagcgcgtg catgtcgtcg agcgatgcga tcggaacgcc
    20341 gaccttgccg acttccggac gcgcgagcgg atgatcagcg tcgtagccgc actgcgtggg
    20401 aagatcgaat gcgatcgaga ggccggtctg tcccgctgcg aggttcttgc gatagagctc
    20461 gttcgacgcg cgcgcgctcg agtgacctgc gtacgttctg aagatccacg gttggtcgcg
    20521 gggccggtcg ccgggcgctg gcatcgggcg aattgttact gagcgacgaa gatctgacca
    20581 gcctcgttag gcgtctctgc aggaattcgc cgaaaataca gctgcgagca gtcggcgttg
    20641 atccacggct ctacctcgtc gtcgttcgtg ttgacggtgc tgataccgcc gccttcgtcg
    20701 tcgaacgtgc gcgattcagt gtcttgcgcg accgcaaaga tgtcgctgcg aatgccggac
    20761 agcgcggagt aaacgagctt cgcccgatcc tcggtcatca cggcttggcc cacgcgcgcg
    20821 ccgaggctgt tgaagcctac cgttcgtgcc ctgtcctcca gcgtgaagct gtttggagtg
    20881 ttggccatga tcgcgacctt gttcggtccc tcactcggat cttgcgtcac catccacagc
    20941 acggtgtggc gcacgcgatc gacatcgggg ttcgcatcgc caacgcgaat ccaaacgttg
    21001 ccgggataca cgtcgtagcc ggcgagcgag aacgcgggct ggttcgtatc ctgctgccac
    21061 gtggttcccg tccagtagta acggccgacc tggagcttgt tctgccccgt cggtaccgtc
    21121 gagagcagca tcgcgggctg tagtgggccg gctggatact tcgctccgtt cggtgcgggc
    21181 tcgggaggag ccagcttcgc gcccttcatg cccgcggtca ggcccgtctg gatgttcggc
    21241 gtcggatcgg gctcccaatc ggagccgttc aagtgcaaca cgatcggccg cgtctgcccc
    21301 gccgaatctt tgcccgtgac gagtgccatc gtgccgtcgg cgttgaccga gaagccgcgc
    21361 gcctcttcga caccgacaat cgagacggga gtcaccgacg catacggacc gcacgcgcct
    21421 cgctccggca cgatgtgctg aaggcccacc gcatagtcgc acccgctagc agcgaggagc
    21481 acgagcgcgt tgcggagcac gcaccaagga tatcatcggc gacgtaggct caccgcgagc
    21541 cccgccaatg tgatccccgc cccgatgtac gcccgcggtc cgagcgaaat cgcccgttcg
    21601 aacagcgcat ccgtgagtag cgccacgagc gggaacacga acacgagcgt gctggtgaca
    21661 agcaacgtcg tgcggccgag cagccagaag taaacgagaa acgcgacgac ggtgccgacg
    21721 accgccaagt acaggagcgc gaccgtcggt gcagttgccg gcggccatcg aatcggctca
    21781 cgtgcgacga gcgcgacgat gccgagcacg accgcggtca cgctgagaaa gatcgtcgtc
    21841 gcgacaagac catgcacgcc ctgcccgcgc accttcatga tcacggagta gctcgtcgca
    21901 acaacgacgg agccgagcac gagcaagacg ccgatcgcct gtgcgcgcga cacgtcgagc
    21961 cgatcgagga acaacacgcc aacgcccgcg agcgagatga ccgcaccgat cacatggcgg
    22021 cgcgtgagcc cgctgatctt cagcgccgtc atcatgatcg cgaggatcag cggctgcgtg
    22081 ccgtagacga ccgcggcgag cccacccgga atgtgctctt caccgagata gacgagcgag
    22141 tacgacgctg catcgagcac acccgctgca acgagccacc accacacgcg gcccttcggc
    22201 cacgcgttcg cgcgaatcgc gagcggcacc agcagcagcg cggcgatcgc gaaacgcatc
    22261 gcgaccgaca gcagcgtcgg atagccatcg ggcgcgatgc agacgcggat cgcgtaccac
    22321 gtcgttcccc agatcagcgc acacgcgaca taagcggcga cgatccgggg cacaacgcgc
    22381 acagcatagc tgctctactt cttctgcgga gcggccggcg gcggcatcaa ctcgagcgag
    22441 ttcgcgtagc tcgtcatgtg gacgaccttg ccgtccttga cctgcgcgat gtcgacgaag
    22501 tgcaggttga tgggcttgtt cgtcgccttc atcgggccga ggttgccctt gagcgtgccg
    22561 tgcagattca cttcgctgac gacgagatcg cctgcggcaa gctgaacgat cttgtcttgc
    22621 ttcagatccg gcaccgcttt gaagaacacg ccgaggtatt ccttgatgcc cttcgtaccc
    22681 tgacgcgacg ccggctcggt gtagtcatcg ccgccaccgt tcggatccat gtacttcagg
    22741 acctcgtccg ccttgtgcgc gtcgatcgcc gcgtagaccg cgttcacgac ctcgaggttc
    22801 ttcgcttcat cgggcgagcc cgtcgaagcc accgttgccg gcttctctgt cgggagctcc
    22861 gcgacgggcc ggaacgttcc cttcttcgcc ttcggatcga gctgggcgtc ctgtgtcggc
    22921 atgtcccagt agcggtgctc ttccttgatg agccccgcat cggagaactg caggacggaa
    22981 acgccgtgca caccgacaga acgcttcgtc gcgttcgtac ccggcgtatc acccgtgttc
    23041 gttcccgtga ccgtccattc aacgacggcg atgttgtcct tcatgatcgc gcgccgcacg
    23101 ccggttttga agtccgggta gccgacgaac gtgttcgctg cttgggcctt gatcgcctcg
    23161 cgcgaggtcc gctcgggcat cgggtagatg cgtaggatcg cgtcggcctc gtactgattg
    23221 gcgatcgtct ccgcctcgtg cgcgttccac gcgatgaaca tcgcacgtgt cgtcttctcc
    23281 atcctctggg ccatcgacat cggcggaggc tcctcggcct tcgcctgcgg cgcggtcgct
    23341 tgctccgcgt tcgatgcgac tgccgctgct gccggatccg gcttcgccgc aggcttcttc
    23401 cccccaccac cgcacgccgt caacagcact gccaccagcc acgtacacgt ccgcatagag
    23461 cctccctttt gaggcccttc gtagatcagc ccagcgtggc gcgcaagccg tcgacgagct
    23521 tggtttcagc gacgacgccg agcgcatcgc gcagacgatc gacgcgtgcg cggg
//
LOCUS       DATLBZ010000492         4157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_646628, whole genome shotgun sequence.
ACCESSION   DATLBZ010000492 DATLBZ010000000
VERSION     DATLBZ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646628"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2044)
                     /locus_tag="VMZ53_24880"
     CDS             complement(<1..2044)
                     /locus_tag="VMZ53_24880"
                     /inference="COORDINATES: protein motif:HMM:NF023400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HUS31770.1"
                     /translation="MAALLAACQNKSKLDQWPSGDSGPRVLSEDKTEQGKGVDAPPAR
                     DPVTAVKLNPVIHEFGTDNVVPTAIVIQLAAPVVDRADVGQRTDKTVVKLTPDVKGSV
                     TYTGVSELTFTPSTAFAFDTSYKVDLVSVETRDGVMNMPPKDQWGENWTYTFKTPKFA
                     FLSWEPADLDLTKHQATMELRFSGPVLANVARTSMTFTIDGKAPTTVNVLSAREPNLV
                     TVQMKDARIAVGAKIGVAIKKGVPATNGATLADAATAEFQVSSDKAISMKHATVVEGA
                     NGFYIEVVCDDKAAEPGSRSSYEGDGYYNLSQRCQLADASRITFTPAVKKVYITSGRA
                     GFRIFGDFKRGVYKMKIGGGSTSVDGGVLLASYTHTFSVAARKPTLSFASSGRYLPRS
                     AWNNLGIKGINVDKVNLVVRHVPAENLVFWLGNDSYETADERTSDVILKKEIPLRSTP
                     DEMTANWLDVGAMLPANTKGVLELRVNGVGATAVSRLLLTNMSLVAKKTPTPDKPWQQ
                     RVQVWALDMDANSLLGDVEVSLVRKSGKAVARCTTSSGSGCTLQTRADDDIDESEPFA
                     LIARRGDDLTYIRYKDLRADVTESSTSGVPYIASTPYRASIFSDRGVYRPGDNAHVVA
                     IVRDQKDKAPDQALPVEVKLVDPRARVAKRITLKTNAAGVLAFDYDLPAFADTGHWR"
     gene            2181..3887
                     /locus_tag="VMZ53_24885"
     CDS             2181..3887
                     /locus_tag="VMZ53_24885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31771.1"
                     /translation="MASDETDALTEIDHASEPRVDLEQTSVIDLRQERYERLGKIGRG
                     GMGEVVLCKDTRIVRQVAMKQLRSKYKNDPAHRERFLFEARVQGQLEHPAIVPVHDLG
                     ETDDGELFFTMKRIRGVTLAKAMDQVRDGVGTYTRRRLLTAFSSICLAVDFAHTRGVV
                     HRDLKPQNIMLGDFGEVYIIDWGIAKLIGHEETPISELVDFPGSDSSSTQAGAVLGTP
                     LYMAPERREGIATPGTDVYSLGVILGQILETEDDIAPELGSISRRASEQDPTYRFKSA
                     RELHDAIERFLDGDRDLEARRKLAQDHVARAEAALASRSADARTAAARDVGRALGLDP
                     SNAAALRILMRLMSDMPAELPPAAQQEIDRRWFERRARTMRIGSLSTLSVLLMTPFIL
                     ASGVLNWTYFAAYVGCVCGASALQWNASRTPNHFYFASAFCLQIIACSTLTWSLGLVG
                     LVPATFTLATVAWRQNVRGLGAALLMLAFVLACLIAPIALASWGITAPFFAVRNHALV
                     ILPVMHDFSPITTTIYATFATFGVIAASSIYGRLFVSEIRQAEAKLTFQAWQLQQLLP
                     PV"
     gene            3897..>4157
                     /locus_tag="VMZ53_24890"
     CDS             3897..>4157
                     /locus_tag="VMZ53_24890"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUS31772.1"
                     /translation="MPAIDPAEIPSKSASGYPEPFRSRVLPREKRALGDALGLTKVGV
                     NLTTLPPGKESSMRHWHTHEDELIYVLEGEVVLVTDDGELVLR"
BASE COUNT          687 a         1428 c         1289 g          753 t
ORIGIN      
        1 cgcgccagtg gccggtgtcc gcgaatgcgg gcaggtcgta gtcgaaagca agcacgccgg
       61 cggcgttggt cttcagcgtg atgcgcttcg caacacgagc ccgcggatcg acgagcttca
      121 cctcgacggg cagcgcttgg tccggcgcct tgtccttctg atcgcggacg atcgcaacga
      181 catgcgcgtt gtcaccgggg cggtagacgc cgcggtcgga gaagatgctc gcacgatacg
      241 gcgtcgatgc gatgtacggc acgcccgacg tgctcgattc cgtcacatct gcacgcagat
      301 ctttgtagcg gatgtacgtg aggtcgtcgc cgcggcgcgc gatcagagcg aatggctcgc
      361 tctcgtcgat atcgtcgtcg gcgcgtgtct gcagcgtgca gccggacccg ctcgacgtcg
      421 tgcacctcgc gactgccttg ccgctcttgc gcacgaggct cacctcgaca tcgccgagca
      481 gcgagttggc atccatgtcg agtgcccaga cctgtacgcg ctgctgccac ggcttgtcgg
      541 gcgtcggcgt cttcttcgcg acgagggaca tgttcgtgag caagagtcgc gatacggcgg
      601 tcgcaccaac gccgttgacg cggagctcga gcacgccctt tgtattcgcg gggagcattg
      661 cgccgacgtc gagccagttg gccgtcatct catcgggcgt gctgcggagc ggaatctcct
      721 tcttcaggat cacgtcgctc gtgcgctcgt cggcggtctc gtagctgtcg ttgccgagcc
      781 agaagacgag gttctcggcg ggcacgtgac gcacgacgag gttgaccttg tcgacgttga
      841 tgcccttgat gccgaggttg ttccacgcgc tgcgcggcaa gtagcggccg ctcgacgcga
      901 acgagagtgt cggcttgcgc gcggcgacgg aaaacgtgtg cgtgtagctc gcgaggagca
      961 cgccgccgtc gacgctcgtc gagccgccgc cgatcttcat cttgtacacg ccgcgcttga
     1021 agtcgccgaa gatgcggaag cccgcgcggc ctgacgtgat gtagaccttc ttcacggcgg
     1081 gcgtgaacgt gatcctcgac gcatcggcga gctggcaccg ctgcgagagg ttgtagtagc
     1141 catcgccttc gtagctcgag cggctgcccg gctccgcggc tttgtcgtcg cagaccactt
     1201 cgatgtagaa gccgttcgcg ccctcgacga cggtcgcgtg cttcatcgag atcgccttgt
     1261 cgctcgagac ctggaactcc gcggtcgctg cgtccgcgag cgtcgcgccg ttcgtggccg
     1321 gaacgccctt cttgatcgcg acgccgatct tcgcgccgac tgcgatacgc gcgtctttca
     1381 tctgcaccgt cacgagattc ggctcgcgtg ccgagagcac gttgacggtc gttggcgcct
     1441 tgccgtcgat cgtgaatgtc atcgaggtgc gcgcgacatt ggcgagcacc gggcccgaga
     1501 agcgcagctc catcgtcgcc tggtgcttcg tcagatcgag gtccgccggc tcccacgaca
     1561 ggaacgcgaa cttcggcgtc ttgaacgtgt acgtccaatt ctcgccccac tgatccttcg
     1621 gcggcatgtt catcacgccg tcgcgcgtct cgacgctgac gaggtcgacc ttgtacgacg
     1681 tgtcgaatgc gaacgccgtg ctcggcgtga acgtcagctc gctgacgccg gtgtacgtaa
     1741 cggagccctt cacatcgggc gtcagcttga cgaccgtctt gtccgtgcgc tgcccgacat
     1801 ccgcgcgatc gaccaccggc gctgcgagct gaatgacgat cgcggtcggc acgacgttgt
     1861 cggtaccgaa ctcgtggatg acggggttca gcttgaccgc ggtgacgggg tcgcgcgcag
     1921 gcggcgcatc gacgccctta ccttgctcgg tcttgtcctc gctgagaacg cgcggcccgc
     1981 tgtcccccga cggccactga tcgagcttgg atttgttctg acatgcggcc aggagagccg
     2041 ccaccgccac gctgcctaac gccacggacg tcgtgaatcg catgagcttg tcctctattt
     2101 gcgccagaac gccggtcccc atgccggcat tccaagcaac ggcgagaaca tacactcttg
     2161 gagcggttac gatcggctcc atggcgagcg acgagaccga cgccctcacc gagatcgacc
     2221 acgcgagtga accgcgcgtc gacctagagc aaacgtctgt gatcgactta cgccaagagc
     2281 gctacgagcg cctcggcaag atcgggcgcg gtggcatggg cgaagtcgtg ctctgcaaag
     2341 acacgcgcat cgtgcgccag gtcgcgatga agcagctgcg ctcgaagtac aagaacgatc
     2401 cagcgcatcg cgagcgcttc ttattcgaag cacgcgtgca gggccagctc gaacatccag
     2461 cgatcgtccc ggtgcacgac ctcggcgaga cggacgacgg cgagctgttc ttcacgatga
     2521 agcgcatccg cggcgtgacg ctcgccaagg caatggatca ggtccgcgat ggcgttggca
     2581 cgtacacgcg ccgccgccta ctcacggcgt tctcgagcat ctgcctcgcc gtcgatttcg
     2641 cgcacacgcg tggcgtcgtc caccgcgatc tcaagccgca gaacatcatg ctcggcgact
     2701 tcggcgaggt ctacatcatc gattggggca tcgcgaagct gatcggccac gaggaaacgc
     2761 cgatctccga gctcgtcgat tttccgggca gtgacagctc ctcgacgcag gcgggggccg
     2821 tcctcggaac gccgctctac atggcgcccg aacgccgcga aggcatcgcc acgccgggaa
     2881 cggacgtcta ctcgctcggc gtcatccttg gacaaatcct cgagaccgag gacgacatcg
     2941 cacccgagct cggctcgatc tcgcgacgcg caagcgagca agatccaacc taccgcttca
     3001 agagcgcccg cgagctccac gacgcgatcg agcggttcct cgacggtgat cgcgatctgg
     3061 aagctcgtcg caagctcgcg caagaccacg ttgcacgcgc cgaggccgca ctcgcaagcc
     3121 gcagcgccga cgcgcgaacg gctgccgccc gagacgtcgg tcgtgccctc ggcctcgatc
     3181 cgtccaacgc agccgcgctg cgcattctca tgcggctcat gtccgacatg ccagccgagc
     3241 tgccgcccgc agctcaacaa gagatcgacc ggcgctggtt cgagcgacgc gcccgcacga
     3301 tgcgcatcgg ctcgctgtcc acgctctcgg tcttgctgat gacgcccttc atcctcgcga
     3361 gcggagtcct caactggacg tacttcgccg cctacgtcgg ctgcgtgtgc ggcgcctctg
     3421 cgctccagtg gaacgcatcg cgaacgccaa accacttcta cttcgcatcg gccttctgtc
     3481 tccagatcat cgcgtgctcg accctcacgt ggtcgctcgg tctcgtcggt ctcgtcccgg
     3541 ccaccttcac actcgcgacg gtcgcatggc gccaaaacgt tcgcggcctc ggcgccgcgc
     3601 tcctcatgct cgcgttcgtc ctcgcgtgcc tgatcgcgcc gatcgcgctc gcgagctggg
     3661 gcatcaccgc gccgttcttc gccgtccgaa accacgcgct cgtcatcctg ccggtcatgc
     3721 acgacttctc cccaatcacg accacgatct acgcgacgtt cgcgacgttc ggcgtcatcg
     3781 ccgcctcatc gatctacggc cgcctcttcg tcagcgagat ccgccaagcc gaagcgaagc
     3841 tgacgtttca agcctggcag ctccaacagc tcctgccgcc ggtgtagatc caacgcatgc
     3901 ccgcgatcga tcccgctgag atcccgtcga agtcggcgtc cggctacccc gagccattcc
     3961 gctcgcgggt cctgcctcgc gagaagcgcg cgctcggcga cgcactcggc ctgacaaagg
     4021 tcggcgtcaa cctcacgacg ctgccgccgg gcaaggaatc ctcgatgcgt cactggcaca
     4081 cgcacgagga cgagctgatt tacgtgctcg aaggcgaggt cgtgctcgtc accgacgatg
     4141 gcgagctcgt cctccgc
//
LOCUS       DATLBZ010000493        17044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10760910, whole genome shotgun sequence.
ACCESSION   DATLBZ010000493 DATLBZ010000000
VERSION     DATLBZ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17044
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10760910"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1554)
                     /locus_tag="VMZ53_24895"
     CDS             complement(31..1554)
                     /locus_tag="VMZ53_24895"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HUS31773.1"
                     /translation="MRAASSLGLCIFVFGCGTTVGPQDAPTARVRVAHLSPDAPDVDF
                     CLAPHGTTDFIGPVLGANGGPLGMAYGQVTKYFDLEAQQYDVRLVAPGAQDCAKGLVP
                     DFTDLPELTDGATATIAAIGKLGHGSTAAFTLRAYIDDAVIEPGKSKLRFIHASPGTP
                     GVDVGIGGGVLFTPVFSNIEYGSASSITAAPAAGIELSARAHETTSDVLSIKPAALTS
                     DANATAIAIGEIGNADAPLQVLLCNDSSPPHGLHTECNLVGGAPERARIRVAHLSPDA
                     PAVDVCLSQTGANRWQGPLLASLGAPTGLSYPQITTYVSVPITTYDVRVVLAGASTCA
                     TPAVADTVGIGVAPGISATIAALGDLARENDASLSDPGFRLKAFVDDTTVDPAKAKLR
                     FIHASPGTPAVDVGLGTSTSWSKVFANVAFSSVATNSPMNDRGYVETAPFTSPVSARL
                     VGSSADALVMHVQVTAGSINTAFAIGGKSGSTTNPLRVLLCRDSAQPLGLLTTCHTN"
     gene            complement(1645..3429)
                     /locus_tag="VMZ53_24900"
     CDS             complement(1645..3429)
                     /locus_tag="VMZ53_24900"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31774.1"
                     /translation="MSEKETPTVVDKPAGDRPAATGSSAKVVPAALGSASIASGTSGA
                     SGVSRPNASFASGVSRPGTRIPSATATISEQAMAAVEAERMRGLLNGIAGSSAFVTLI
                     VLLVSGDPWAQRVHAAALGVTAVMCATLALWFRNPKRYRPRVALYAILSQVVVLATGY
                     YFWGFFSTYGALVPLTVYIAMGSATKTETILGTALLVSVQTSLGLALTLGYITPRGLV
                     EPVVGRTDFKTQLIALALIQGITIGAAVAGRAAKRASQAALETHNQALLDLARREAQL
                     AEAWADARAAREAGVGGLGRFTDQRIDDFELGEILGRGAMGEVYAAKTPGNHPVAVKI
                     LAPHLLRDPSARDRFLRESEIVSSINSPHVVKVLTVAPKDALVPYIAMERLEGTDLAQ
                     MLKKRSLLPLAEIEDIVTQVATGLDAAHKAGVIHRDLKPSNIFATGEASARVWKLLDF
                     GASKWHDGEGTLTRDNVVGTPGYMAPEQALGRAVDQRSDVYALGVIMYRLMTGVPAVV
                     PGEVPAMLQEVSFKMPVQPSKRAKVSPALEAVLAVALAKSPVHRFSTAGDLAAAFRAA
                     AAGKLDRAIQQRAEAVLADLPWDAWNRR"
     gene            complement(3430..3621)
                     /locus_tag="VMZ53_24905"
     CDS             complement(3430..3621)
                     /locus_tag="VMZ53_24905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31775.1"
                     /translation="MRRRGRILAIKWGMNPNSSSLGVDVTFLLVGATALAIVTPVIGA
                     LLRWKKPATVEAPPAPRED"
     gene            complement(3634..4629)
                     /locus_tag="VMZ53_24910"
     CDS             complement(3634..4629)
                     /locus_tag="VMZ53_24910"
                     /inference="COORDINATES: protein motif:HMM:NF019840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31776.1"
                     /translation="MSRSFVSCFIVVTACTSASGPIGIDPDEASGDDKADGAGRIVDV
                     QCADMPDAGATKEWRHFKSKVITAASDGHHSGIDLVATDADEEQVIRGDISYTIFDKA
                     LEDEDVDVFACRRRAWVRLGTVRTDGDGQFALTLTGDDRLPIGIRDMAVSVAGDRSTG
                     RFLAVVAPEGTELAVSDVDGTLTTSEEAFAANAFGIDAAIHDGAAEGWMHVRSTGRIP
                     VYVTARARMFTSDTREWLAKKGMPRGVLRLAPQVLLPGSATVDYKSETLAELPLPIAL
                     GVGNRASDIEAYTNAGVAADRIFIKLPEFSGEVASHLQAGEGVGFSAYPGPLANL"
     gene            4829..5296
                     /locus_tag="VMZ53_24915"
     CDS             4829..5296
                     /locus_tag="VMZ53_24915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31777.1"
                     /translation="MKSLVVFTLVMLTAGRAHAGGAAFPNPGTANVLAGPTVGIAWGH
                     GHTRHFSLGFEAGVGYMVERANVGIVRRADESLVYITLDPWLFVGATLGAGYGTESGF
                     TPILGVWEGAPLGLQCEGRSSGDWATQGTISISYRWSGVHEILFSPKFGKERC"
     gene            complement(5312..6412)
                     /locus_tag="VMZ53_24920"
     CDS             complement(5312..6412)
                     /locus_tag="VMZ53_24920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31778.1"
                     /translation="MARALVILLLLCRIAAADPNALLEGLDTDSKPELAAAVTAIERA
                     PTTPELADVLFAAGRACEDRLLDPARALALYERILRELPDASVAIAAERRVEKLQSVR
                     GHEKLASELAELIAHADDRHPAMVWLEGEALASSPWPGAPDAALWLGDWMCRTQQYQA
                     AQLRYARLRESWPDSEQARIAHRNAAGCALDAHDFALATRLSNELVVGDDVDAAVKSD
                     LLAAARRGRFREALYTASWIALLLASAVLLASLADAILRGGRRLPALRPPIEVLFLAP
                     LALVIVAGSYLSQRSIAPAVLRISLAGLTAAYLSGLTLDLLRLRGRPVRMRSTIHVIA
                     CAFGIVAIGYIAITRDGLLDMLVETVKYGPGD"
     gene            complement(6415..7890)
                     /locus_tag="VMZ53_24925"
     CDS             complement(6415..7890)
                     /locus_tag="VMZ53_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS31779.1"
                     /translation="MSEESTQGNGEPILIVDDEKNIRRTLRMVLEGEGHAVHEAGSIA
                     EAETVLARDNIDMILLDVKLGDDNGLDLLRSLKSRGEDGMSSRTSEIPVVMISGHATI
                     EDAVAATRLGAFDFMEKPLDRNRVMVTVRNALERRKMWREVHDLRRAVDARWELLGNT
                     PVMNELRRQIAKVAPTRSRILITGESGTGKELIARAIHRNSSVSAGPFVKVNCAAIPP
                     ELIESELFGHERGAFTGAVAKKRGLFEVADGGTIFLDEIGDMALPAQAKVLRVLQSGE
                     LTRVGGEKSMRTDCRVVAATNRDLEQMVKDGTFREDLYFRLNVVPIRSPDLKERADDI
                     PLLVESFVRECCDENGLGYKPVDEPVFERLKQYEWPGNVRELRNVVERLVIMSDEVIR
                     EKDLPPYLGGPRPPGQGSKANQSGPVTAIDLGRYAGKSLREFREEVESEFIRIRLAEF
                     EWNISRTAQALGIERTNLHKKLRALGIHRGEGSGPVGGDNE"
     gene            complement(7883..8704)
                     /locus_tag="VMZ53_24930"
     CDS             complement(7883..8704)
                     /locus_tag="VMZ53_24930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31780.1"
                     /translation="MKRAFVIVAMMVGAAHAGNAPLVTPPAGWKQDAQQATVLAGKAN
                     EVTHFGGLRSLATSDVYVAPEGGAVLIVTAVAGKVTAADRDAAARFAVDELLDASSRA
                     TLSGGGITVDSSGSRVVTDAKMVRGDIQWTDKSTGTRTNALVEVVADEENIVAITGEC
                     VLGNVDVKLAAACTGALTTLDPGIPLAKRQPIALAPSGTLLKAQPVPTQTMGRPPATM
                     TDGTHTPLPPMTVPTEKPARDLRPVYVGLGIVLLAAAFWWNQRQRAKFEKDSDDE"
     gene            complement(8701..9216)
                     /locus_tag="VMZ53_24935"
     CDS             complement(8701..9216)
                     /locus_tag="VMZ53_24935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31781.1"
                     /translation="MLVAALPLAACRASEAGPGSGTGSAASSGLSAPAGWKALPSLAN
                     AAHDAAKTSGLAVDSAEAWGETARGCYGTWLSLTTTRAKPLKLADEFVKGIETEPSLA
                     GIALTEVVKPAATTDNDMLSLAFAKPPYRGKVRARLVNDGHITALVCFWNEREPAACE
                     AACGQLLGGLK"
     gene            complement(9216..10244)
                     /locus_tag="VMZ53_24940"
     CDS             complement(9216..10244)
                     /locus_tag="VMZ53_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HUS31782.1"
                     /translation="MAGSFRIRQSYREDVDQVLAVAEHLDTVNLPHDRQVIEGILDRS
                     EKSFNGEVEGSDLEFVFVLEDLAQKRIIGTSMIYAQHGTKRAPHIFFRVENDERYSVT
                     LDRYFVHRTLRIGYNYNGPTEIGGLILLPEYRRNVHALGKALSYVRFLFMRMHRPLFR
                     NQVLSELLPPLEADGTSKLWEALGRRFTGLTYQEADRVSKDNKEFIHALFPDDPIHTE
                     LLPEDVQAIIGQVGEETRAVEKMLRRIGFDYAEQIDPFDGGPHFLANTDDITIVKDAM
                     EVEVRAEAIGDRRWAIVGVETPGSRPGFRATGALVTPHGGGAVGLPEEMRNRLGVEVG
                     QKVWLSYG"
     gene            complement(10281..10760)
                     /locus_tag="VMZ53_24945"
     CDS             complement(10281..10760)
                     /locus_tag="VMZ53_24945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31783.1"
                     /translation="MQQVKQNVQIADDLALQFQAVAGRIAAAMASQQDSDLSALRAES
                     ERLYAGVWDHLDTAAAQTANAGRSTAAYATIRSSADTAVGVTGVASEITNVEHGYKKT
                     TYTVQGSAKLNAQGIESARQASAALKAAWPDVDWTPPAPLGDIDLRPRGFFARLFGR"
     gene            complement(10821..11648)
                     /locus_tag="VMZ53_24950"
     CDS             complement(10821..11648)
                     /locus_tag="VMZ53_24950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUS31784.1"
                     /translation="MRALFLPLFAALVSTADAKGTPKVPKPPPLAWSGPAAGPTLTGD
                     PELIFTFDDGPDPKRTPFVLDALAAHHIHAIFFMVGARLEAPEAQAIVDRILADGHII
                     ANHTMTHQDLCHLKDDQRANDEIDKGQKIIEAAAKMQMVWFRAPYGVRCELLDTMLAA
                     RGITHFHWDLDPKEWKHGSTKKTIDYLEKMLGKIQGRNVLLMHDIKKATVEALPVILD
                     WLEQENARRKELRIRRIRIIQGYELAIERLPPGLLDWLSEVAPDRQRLAQAVTSVLP"
     gene            11708..12733
                     /locus_tag="VMZ53_24955"
     CDS             11708..12733
                     /locus_tag="VMZ53_24955"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HUS31785.1"
                     /translation="MLERLENKEPPRIRLANTPTRGHWLRYGEQLGTKIWLKRDDHTG
                     SELMGNKVRKLEYLMAEALQLQATHVITCGGEQSNHARATAMAAAHLGLKSVLVLRTD
                     TPEKPPATTGNILLDRLVGAEIRWISRAAWKDRNRLLAEEAERVKSAGGRPYIIPEGG
                     SNALGSWGYLRAMRELVEDLAGIAAPDNPVTVVYACGSGGTGAGLIVGWKLLGLQALG
                     IRVTGIAVCDDKPYFVDSIGKICGDFEERWQLDAHVTPQDIDIIDSHVGLGYAKSRPE
                     ELATIRDVCRSDGIVLDPVYTGKAFHGVVTELKKDPKRFGAAVAFIHTGGMFGLFASP
                     DQIGSVL"
     gene            complement(12730..13293)
                     /locus_tag="VMZ53_24960"
     CDS             complement(12730..13293)
                     /locus_tag="VMZ53_24960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31786.1"
                     /translation="MFRPLLVLAVVSVPAFAHADGNLELGPTLAVASESSDVRSAGHP
                     GIALSGASWSGPVGVTAEVGHQPWAWDSSSNWLSVGGRISPVSSPKRICDRRSCTSLR
                     PWLELGIAREMWMLEEPGIPHSSFSRFSGRAGAGIDVLRSDMGGTIWFRVQHANDRPT
                     IMGPFGEAREPYDTSLVVGLGVFFAAI"
     gene            complement(13334..13474)
                     /locus_tag="VMZ53_24965"
     CDS             complement(13334..13474)
                     /locus_tag="VMZ53_24965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HUS31787.1"
                     /translation="MTILIVLLLLALLGGGFGYSRFGAAGLGPAGLIILILVILAITG
                     RL"
     gene            complement(13571..14452)
                     /locus_tag="VMZ53_24970"
     CDS             complement(13571..14452)
                     /locus_tag="VMZ53_24970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS31788.1"
                     /translation="MTRLQGKIAVVTGASRGGGRGVAVELGAAGATVYVTGRTSRSHA
                     PPAYERMLAQGHLEKMPGTIEDVAEEVTAAGGRGIAVRCDHSDVAQVRELFARVDREQ
                     GQLDLLVNNAWGGHETLTADQLSAPFWELSIDHWESMFDHGVRNHVVASHAAAPLFVR
                     QRRGLIITTSFWDRGRYIKGNLFYDVAKATMNRFAFALAEELRAHEVTSVALVPGWMR
                     TEIILAAFSTDERGWTDVPPLARTESPRYLGRAVVALASDPHAHQKTGEVLYVGELAA
                     RYGFTDVDGRVIPPFEM"
     gene            14533..15522
                     /locus_tag="VMZ53_24975"
     CDS             14533..15522
                     /locus_tag="VMZ53_24975"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HUS31789.1"
                     /translation="MTRVLLLSLVLAACSTEPKTTSASKEVKLLNVSYDPTRELYADI
                     NAAFAKQWEAKTGQHVTIEQSHGGSGKQARAVIDGLEADVVTLALAYDVDAIAKAGLI
                     KPDWQTKLPESSTPFTSTIVFLVRKGNPKGIHDWADLVKPGVEVITPNPKTSGGARWN
                     YLAAWGYTLQTGGDEAKAKAFVADLYKHVPVLDTGARGATTTFAERGIGDVLIAWENE
                     AYLAINKLGADKVEIVVPSVSILAEPPVAVVDDVVDKRGTREVATAYLTFLYSPEAQQ
                     IAAKQFYRPRDTTVQTSFADVKLFTIDQVFGGWAKAQPAHFNDGGQFDAITAK"
     gene            15523..16350
                     /gene="cysT"
                     /locus_tag="VMZ53_24980"
     CDS             15523..16350
                     /gene="cysT"
                     /locus_tag="VMZ53_24980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HUS31790.1"
                     /translation="MARRSAIPGFGLTMGYTIVYLSLIVLIPLATLAIKTAQLSPSEI
                     WELATSKRAIASYKITFGISLLAALLNVVFGSLLAWTLVRYRFFGKSILDAIVDIPFA
                     LPTAVSGLALTAVLAPNGWIGRFLEPLGIRAVFNPLGVLIALMFISLPFVVRTIQPVL
                     EEIEPEVEEAAATLGATRWQTLYRVILPGLWPAMVTGFALAFARAIGEYGSVVFISGN
                     KQYSTEITPYLIMTKLDEFDFASATALALVMLAVSFAMLLAINGFSAWVRRRQGAFA"
     gene            16350..>17044
                     /locus_tag="VMZ53_24985"
     CDS             16350..>17044
                     /locus_tag="VMZ53_24985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HUS31791.1"
                     /translation="MVTSAPPIAERVVAASTSEPVWARRILIGISVGFSALFILVPLA
                     AIFGEAFSRGLRAFGQALVDPKALSAIKLTLITTAIVVPLNLVFGVSAAWAIARFRFW
                     GRSFLISLIDLPLAVSPVIAGMVFVLLFGSRGGFLGPWFAAHGVKIIFGLPGIVLATT
                     FVTFPFIARELIPLMEAQGSDEEQAAVSLGAGGWTTFRRITLPNIKWGLLYGVLLCSA
                     RALGEFGAVAVVAG"
BASE COUNT         2820 a         5699 c         5599 g         2926 t
ORIGIN      
        1 gcatctgcat atggcgggcg gaagctaaca tcagttggtg tggcaggtgg tcagcaggcc
       61 gagcggctgc gcgctatcgc ggcagagcag cacgcgcagc ggattcgtcg tcgagcccga
      121 cttgccgccg atcgcgaatg ccgtgttgat cgatccggcg gtcacctgca cgtgcatgac
      181 aagggcgtcg gcggacgagc ccacgaggcg cgcgctcacc ggtgacgtga acggtgcagt
      241 ctcgacgtaa ccgcgatcat tcatcgggct attcgtggcg acgctcgaga acgcgacgtt
      301 cgcgaacacc ttcgaccagc tcgtgctcgt gccgaggccg acgtcgacag cgggcgtgcc
      361 gggcgacgcg tggatgaagc gcagcttcgc cttggccgga tcgaccgtcg tgtcgtcgac
      421 gaatgccttc aggcggaagc ctggatcgct cagcgaggcg tcgttctcac gtgcgaggtc
      481 gcctagtgca gcgatcgtgg cgctgatgcc gggggcgacg ccgatgccga cggtatcagc
      541 gaccgccggt gtcgcgcagg tcgacgcacc tgcgagcacg acgcgcacgt catacgtcgt
      601 gatcggaacc gacacgtacg tcgtgatctg cggatacgag aggcctgtcg gcgcgccgag
      661 cgatgcgagc agcgggcctt gccagcgatt cgcgccggtc tgcgagaggc agacgtcgac
      721 ggcgggtgca tccggcgaga gatgcgcgac gcgaatgcgt gcacgctcgg gcgcgccacc
      781 gacgaggttg cactccgtgt gcaggccgtg cggcggcgag ctgtcgttgc agagcagcac
      841 ttgcagcggt gcatcggcat tgccgatctc accgatcgcg atcgcggtcg cgttcgcatc
      901 ggacgtcagc gccgccggtt tgatcgacag cacatcgctc gtcgtttcgt gcgcgcgagc
      961 cgacagctcg atgccagctg cgggcgccgc cgtgatcgac gacgcggatc cgtactcgat
     1021 gttcgagaat actggcgtga acaacacgcc gccgccgatt ccaacgtcga cgcctggcgt
     1081 acccggcgat gcgtgaatga agcggagctt ggatttgccc ggctcgatca ctgcgtcgtc
     1141 gatgtacgcg cgcagcgtga aggccgcagt gctgccgtga cccagcttgc cgattgctgc
     1201 gatcgttgcc gtcgcgccat cggtgagctc gggcagatcc gtgaagtcgg ggacgagtcc
     1261 cttcgcgcaa tcttgtgcgc cgggtgcgac gagacgcacg tcgtactgct gcgcctcgag
     1321 atcgaaatac ttcgtgactt ggccgtacgc catgccgagc ggtccgccat ttgcgccgag
     1381 caccggcccg atgaagtccg tcgtgccgtg tggggcgaga cagaagtcga catcgggtgc
     1441 atccggcgag agatgcgcga cgcgcacgcg tgcggttggc gcgtcctgcg gaccgacggt
     1501 ggtgccgcaa ccgaaaacga agatacaaag accgagcgaa gaagcagccc tcatcgagac
     1561 tcctttacgc cgctgaagaa gcggttcgag agtctgttcg ccgggccgac gagattggat
     1621 gcagcagcta actaggcgtg gcgcctagcg acgattccac gcatcccagg gaagatctgc
     1681 gaggaccgct tccgcgcgct gctggatcgc gcgatcgagc ttgcctgcgg ccgccgcgcg
     1741 aaacgctgca gcgaggtcgc ccgccgtcga gaatcgatgc acgggagact tcgcgagcgc
     1801 gaccgcgagc accgcctcga gagctggcga caccttcgcg cgcttgctcg gctgcaccgg
     1861 catcttgaac gacacctctt gcagcatcgc gggaacctcg ccgggaacaa ccgcgggcac
     1921 gcccgtcatc aagcgataca tgatcacgcc gagcgcgtac acgtcgctgc gttgatcgac
     1981 cgcgcggccg agcgcttgct ccggcgccat gtagccgggc gtgccgacga cgttgtcgcg
     2041 cgtcagcgtg ccttcgccgt cgtgccactt cgatgcgccg aaatcgagga gcttccacac
     2101 gcgcgcgctc gcttcacccg tcgcgaagat gttcgacggc ttgaggtcgc gatggatgac
     2161 gcccgctttg tgcgcagcat cgaggccggt cgcgacttgc gtgacgatgt cttcgatctc
     2221 cgcgagcggc agcagcgagc gcttcttcag catctgcgcg aggtcggtgc cttcgagccg
     2281 ctccatcgcg atgtacggga cgagcgcgtc cttcggcgcg accgtgagca ccttcacgac
     2341 gtgcggtgaa ttgatcgagc tgacgatttc cgattcgcga aggaagcgat cgcgtgcgct
     2401 cggatcgcgg agcaggtgcg gtgcgaggat cttcaccgcg acgggatggt tgccgggcgt
     2461 cttggccgcg tagacctcgc ccatcgcgcc gcgaccgagg atctcgccga gctcgaagtc
     2521 atcgatgcgc tgatcggtga agcgaccgag cccgccgacg ccggcttctc gagcggcacg
     2581 cgcgtcggcc catgcttccg cgagctgcgc ttcgcgtcgt gcgagatcga ggagcgcctg
     2641 gttgtgcgtc tcgagtgccg cttggctggc gcgtttcgcc gcacgtccgg cgaccgccgc
     2701 gccgatcgtg atgccctgga tcagcgcgag tgcgatgagc tgcgtcttga agtccgtgcg
     2761 accgacgacc ggctcgacga gacctcgtgg cgtgatgtag ccgagcgtca gcgcgaggcc
     2821 aaggctcgtc tgaaccgaga cgagcagcgc ggtgccgagg atcgtctcgg tcttcgtcgc
     2881 cgagcccatc gcgatgtaca cggtgagcgg cacgagcgcg ccgtacgtgg aaaagaagcc
     2941 ccagaagtag tagccggtcg cgagcacgac gacctgcgag aggatcgcgt acagcgcgac
     3001 tcgcggccga tagcgtttcg gattgcgaaa ccacagcgcg agcgtcgcgc acatcactgc
     3061 cgtcaccccg agcgcggcag cgtgcactcg ctgcgcccac ggatcgccgc tgacgagcag
     3121 cacgatgagt gtcacgaacg cagacgagcc cgcgatgccg ttgagcagac cgcgcatgcg
     3181 ctccgcctcg acagccgcca tcgcttgctc ggagatcgtt gcggtggcgc tcggaatccg
     3241 cgtcccgggt cgcgacacgc cactcgcgaa cgaggcattc ggccgcgaca ctccgctcgc
     3301 gccgctcgtg ccgctcgcga tcgacgcgct cccaagcgct gcgggcacta ccttcgcgct
     3361 cgaaccggtc gctgcgggcc ggtcgccggc aggcttgtcg acgacggtcg gcgtctcctt
     3421 ttcggacact cagtcctcgc gcggcgccgg cggtgcttcc accgtggccg gcttcttcca
     3481 ccgcagcagc gcgccgatga ctggcgtcac gatcgcgagc gccgtcgcgc cgacgagcaa
     3541 gaacgtgacg tccacgccga ggctcgacga gttggggttc atgccccact tgatggctaa
     3601 gatccggccg cgccgacgca cggcaccatt ctatcagagg ttcgcgagtg gaccggggta
     3661 cgcggaaaag ccgacgcctt cgcccgcttg cagatgagat gcaacctcgc cggaaaattc
     3721 cggaagcttg ataaagatgc ggtccgcggc gacgcctgcg ttcgtgtagg cctcgatgtc
     3781 gctcgcgcga tttccgacgc caagcgcgat cggcagcgga agctccgcga gtgtttcgct
     3841 cttatagtcg accgtcgcgc tgccaggcag cagcacctgc ggcgcgaggc gaagcacccc
     3901 gcgaggcatc cctttcttcg cgagccactc gcgcgtatcg ctcgtgaaca tgcgcgcgcg
     3961 cgccgtcacg tacaccggga ttcgcccggt cgaacgaacg tgcatccagc cctccgcggc
     4021 gccgtcgtgg atcgcggcgt cgatgccgaa cgcattggcc gcgaatgctt cttccgacgt
     4081 cgtcagcgtg ccgtcgacat cactgacggc gagctccgtg ccctccggcg cgaccacggc
     4141 gagaaagcgt cctgtgctgc gatcacccgc gaccgaaacc gccatgtcgc ggatgccgat
     4201 cggcagccgg tcgtcgccgg tgagcgtcag cgcgaactgg ccatcgccat ccgtgcgcac
     4261 cgtgccgaga cgaacccatg cgcgccggcg gcacgcgaac acgtcgacgt cttcgtcctc
     4321 gagcgccttg tcgaagatcg tgtacgagat atcgccgcgg atcacttgtt cctcgtcggc
     4381 gtcggtcgcg acgagatcga tgccactgtg atggccatcg ctggcggccg tgatgacctt
     4441 gctcttgaag tgtcgccact ccttcgtggc ccccgcgtcc ggcatgtcag cgcactgcac
     4501 gtcgacgatg cggcccgctc cgtctgcttt gtcatcgccg ctcgcctcgt ccgggtcgat
     4561 gccaatcggc ccactggcgc tcgtgcaggc cgtaacgacg atgaaacaac tcacgaagct
     4621 ccgcgacatg ccgcggccat tctgcagatc gcggaccacg cacttcgatc gacgagccgg
     4681 ccacttagcg cgcgcctgta acgcgactgc aaacaatggg tggcagcaaa gccagccacg
     4741 actcccagac tagtcgccgg gcgtcgatca cgcgctgttc acggcgacgt cctcgtgcat
     4801 tcacgtctgc tccggcgaac gtcgacacat gaaatcgctc gtcgtgttca ccctcgtcat
     4861 gctgactgcc ggccgcgccc atgccggcgg tgcagcgttt ccgaacccag gtacggcgaa
     4921 cgttctcgcc ggaccgacag tgggcatcgc gtggggacac gggcacacga gacacttcag
     4981 cttgggcttc gaagcggggg tcgggtacat ggtcgaacgc gccaacgtcg gcatcgtccg
     5041 ccgcgcggac gagagcctcg tctacatcac gctcgatccg tggctgttcg tcggcgcgac
     5101 tctcggcgcc ggttatggca ccgagtccgg cttcacaccg atcctcggtg tgtgggaagg
     5161 cgcgccgctc ggtctacagt gcgagggacg atcgagcggc gattgggcga cgcaaggcac
     5221 gatctcgatc agttatcgct ggtcgggcgt gcacgagatc ctgttctcac ccaagttcgg
     5281 caaggagcgc tgctagacgc tcgtcggctg gctagtcgcc ggggccgtac ttcacggtct
     5341 cgacgagcat gtcgagcagg ccgtcgcgcg tgatcgcgat gtagccgatc gcgacgatgc
     5401 cgaatgcgca cgcgatcacg tggatcgtcg agcgcatgcg gaccgggcgg ccgcggaggc
     5461 ggaggaggtc gagcgtgagg ccggagagat aggcggcggt gagcccggcg agcgagatgc
     5521 gcagcaccgc tggcgcgatc gagcgttgcg agaggtacga cccggcgacg atcacgagcg
     5581 cgagcggcgc gagaaacagc acctcgatgg gcgggcgcag ggcaggcagc cggcggcctc
     5641 ctcgtaggat cgcgtcggcg agcgatgcga gcaggactgc gcttgcgagc agcaacgcga
     5701 tccatgatgc ggtgtacagc gcttcgcgga agcgaccgcg acgggccgca gcgagcaggt
     5761 cagacttcac ggccgcatcg acgtcatcac cgacgacaag ctcgttcgac agccgcgtcg
     5821 cgagtgcgaa gtcatgcgcg tcgagcgcgc aacctgcggc gttgcgatgc gcgatgcgcg
     5881 cttgctcgct atcgggccac gactcgcgga gccgtgcgta gcgcagctgc gcggcctggt
     5941 attgctgcgt gcggcacatc cagtcgccga gccacagcgc ggcatcggga gctccgggcc
     6001 agggcgagct cgcgagcgct tcaccctcga gccacaccat cgcgggatgg cggtcgtccg
     6061 catgcgcgat cagctcggcg agctcgctgg caagcttctc gtggccgcgc acgctctgca
     6121 gcttttcgac gcggcgctcg gcggcgatcg cgacagacgc gtccggaagc tcgcgcaaga
     6181 tgcgctcgta cagcgcgagt gcgcgtgctg ggtcgagcag ccggtcttcg cacgcgcgcc
     6241 ccgcggcgaa cagcacgtcg gcgagctcgg gcgtcgtcgg tgcgcgctcg atcgccgtaa
     6301 ccgctgcggc gagctctggc ttggaatcgg tatcgaggcc ctcgagcagc gcgttcggat
     6361 cagcagccgc gatgcgacag agcagcagca gaatgacgag cgcgcgagcc acgcctattc
     6421 gttatcgccg ccgaccggtc cactgccttc gccacggtgg atgccgagcg cgcgcagctt
     6481 cttgtgcaga ttggtccgct cgatgccgag cgcttgtgcg gtgcgggaga tgttccactc
     6541 gaactcggcg agccgaatac gaatgaactc gctctccacc tcttcgcgga actcgcgcag
     6601 gctcttgccc gcgtagcgcc cgagatcgat cgcggtgacc ggaccactct ggttcgcttt
     6661 gctgccttgg cccggcggac gtggtccacc gaggtacggc ggcagatcct tctcgcggat
     6721 cacctcgtcg ctcatgatga cgagacgctc gacgacgttg cggagctcgc gcacgttgcc
     6781 gggccactcg tactgcttga gccgctcgaa caccggctcg tcgacgggct tgtagccgag
     6841 gccgttctcg tcgcagcact cgcgcacgaa cgactcgacg agcagcggga tatcgtcggc
     6901 gcgctctttg agatcgggag aacgaatcgg cacgacgttg aggcggaagt agaggtcctc
     6961 gcggaacgtg ccgtctttca ccatctgctc gaggtcgcga ttggtcgcag cgacgacgcg
     7021 acagtcggtg cgcatcgact tctcaccgcc gacgcgcgtc agctcgccgc tctgcaacac
     7081 gcgcaagacc ttcgcctgcg cagggagcgc catgtcgccg atctcgtcga ggaagatcgt
     7141 gccgccgtcg gcgacctcga acaggccgcg cttcttcgca accgcaccgg tgaacgcacc
     7201 gcgctcgtgg ccgaacagct cgctctcgat cagctcgggc gggatcgcgg cgcagttgac
     7261 cttcacgaac gggccggccg aaacggaaga gttgcggtgg atcgcgcgcg cgatgagctc
     7321 cttgcccgtg ccgctctcgc cggtgattag gatgcggctg cgcgtcggtg cgactttggc
     7381 gatctggcgg cgcagctcgt tcatcaccgg cgtgttgccg agcagctccc aacgcgcgtc
     7441 gacggcgcgc cgcaggtcgt gcacctcgcg ccacatcttg cggcgctcga gcgcgttgcg
     7501 gacggtgacc atcacgcggt tgcgatcgag cggcttctcc atgaagtcga acgcgccgag
     7561 gcgcgtggcc gcgaccgcgt cctcgatcgt cgcgtggccc gagatcatca cgaccggaat
     7621 ctccgacgtc cggctcgaca tgccgtcttc accgcggctc ttgagcgagc gaagcagatc
     7681 gaggccgttg tcgtcgccga gctttacgtc gagcaagatc atgtcgatgt tgtcgcgcgc
     7741 gagcacggtc tcggcttccg cgatcgatcc ggcctcgtgc acggcgtgac cttcgccttc
     7801 gagcaccatg cgcagcgtgc ggcggatgtt cttctcgtcg tcgacgatga ggatgggctc
     7861 gccgttgcct tgggtggact cttcactcat cgtcggaatc cttttcgaac ttggcgcgct
     7921 ggcgctgatt ccaccagaac gccgctgcga gcagcacgat accgagtcca acgtacaccg
     7981 gacggaggtc tctggccggt ttctccgtcg ggaccgtcat gggaggcagc ggcgtgtgcg
     8041 tgccgtcggt catcgtcgcg ggcggccgtc ccatggtctg cgtcgggacc ggctgcgcct
     8101 tcaagagcgt gccggacggc gcgagtgcaa tcggctgacg cttcgcgagc gggatccccg
     8161 gatccagcgt cgtcagcgca cccgtgcagg cggcggcgag cttgacgtcc acattaccga
     8221 gcacgcactc gcccgtgatc gcgacgatgt tctcttcgtc ggcgacgacc tcgacgagcg
     8281 cattcgtccg cgtgcccgtc gatttgtcgg tccattggat gtcgccgcgc accatcttcg
     8341 cgtcggtgac gacgcggctt cccgagctgt cgacggtgat gccgccgccg gagagcgtcg
     8401 cgcgactgct cgcgtcgagc agctcgtcga cggcaaaccg cgccgccgcg tcgcgatccg
     8461 cggccgtgac cttgcccgcc accgcggtca cgatgagcac ggcaccgccc tcgggcgcga
     8521 cgtagacgtc cgacgtcgct agcgagcgaa gcccaccgaa gtgagtcacc tcgttggcct
     8581 tgcccgcgag cacggtcgct tgctgcgcat cctgcttcca gccggcaggc ggcgtgacga
     8641 gcggcgcatt gccggcgtgc gcggcgccaa ccatcatcgc gacgatcacg aacgcgcgct
     8701 tcatttcaat cctccgagca actgtccaca tgcggcctcg catgcagcgg gctcgcgctc
     8761 gttccagaaa cacacgagcg cggtgatgtg gccatcgttg acgagtcgcg cgcgcacctt
     8821 gccgcggtac ggcggctttg cgaacgcgag cgagagcatg tcgttatccg tcgttgcagc
     8881 cggcttcacg acctcggtca acgcgatccc cgcgagcgac ggctccgtct cgatcccttt
     8941 cacgaactcg tcggcgagct tcagcggctt cgcgcgcgtc gtcgtgagcg agagccacgt
     9001 gccataacag ccgcgcgctg tctcgcccca cgcttccgca ctgtcgacgg caagcccgct
     9061 cgtcttcgct gcatcgtggg cagcgttcgc gagagacggc agcgccttcc acccggccgg
     9121 cgcgctcagc cccgacgacg ctgccgagcc tgtgcccgac cctgggcctg cttccgacgc
     9181 gcgacatgcc gcaagcggca gcgcagcgac gagcatcagc cgtacgagag ccataccttc
     9241 tggccgacct cgacgccgag gcggttgcgc atctcttccg gcagtccgac cgctccacca
     9301 ccatgcggcg tgacgagcgc acccgtcgcg cgaaatcctg gccgcgagcc cggcgtctcg
     9361 acgccgacga tcgcccatcg cctgtcaccg atcgcttcgg cgcgaacctc gacttccatc
     9421 gcgtccttca cgatcgtgat gtcgtcggtg ttcgccagga agtgcgggcc accgtcgaac
     9481 ggatcgatct gctcggcgta atcgaagccg atcctgcgca gcatcttctc gaccgcgcgc
     9541 gtctcttcgc cgacctggcc gatgatcgct tgcacgtcct ctggcaacag ctcggtgtga
     9601 atcggatcgt cggggaacag cgcgtgaatg aattccttgt tgtccttcga gacccggtcg
     9661 gcttcctgat acgtcaggcc cgtgaagcgc cggccgagcg cttcccacag cttcgaggtt
     9721 ccatccgctt cgagcggagg caacagctcg ctcagcacct ggttgcgaaa cagcggccgg
     9781 tgcatgcgca tgaacaagaa acgcacgtag ctgagcgcct tgccgagtgc gtgcacgttg
     9841 cgccgatatt cgggcagcag gatgagcccg ccgatctcgg tcggcccgtt gtagttgtag
     9901 ccaatgcgca gcgtgcggtg cacgaagtag cgatcgagcg tgacgctgta gcgctcgtcg
     9961 ttctcgacgc gaaagaagat gtgcggcgcg cgcttcgtcc cgtgctgcgc gtagatcatc
    10021 gacgtgccga tgattcgctt ctgcgcgaga tcctcgagca cgaacacgaa ctcgaggtcc
    10081 gagccctcga cctcgccgtt gaacgacttc tcggagcgat cgaggatgcc ctcgatcacc
    10141 tggcgatcgt gcggcaggtt gaccgtgtcg agatgctcgg caaccgcgag cacctgatcg
    10201 acatcctctc gatacgattg acgaatccga aacgatccgg ccacgactcg ggcaccctac
    10261 catgggcgcc ggccaagcgc ttagcggccg aataggcgcg cgaagaagcc gcgcgggcgc
    10321 agatcgatat cgccgagcgg cgcgggcggc gtccagtcga catccggcca cgcggccttg
    10381 agcgcagcgc tcgcttggcg cgccgactcg atgccctgcg cgttgagctt cgccgagccc
    10441 tgcacggtgt acgtcgtctt cttgtagccg tgctcgacgt tggtgatctc cgacgccacg
    10501 cccgtcacac cgaccgccgt atccgccgac gaacggatcg tcgcgtaggc tgccgtcgag
    10561 cgaccggcgt ttgccgtctg cgccgcggcc gtgtcgaggt gatcccagac gccggcgtag
    10621 aggcgctcac tctcggcgcg aagcgcggac aaatcgctgt cctgctggct cgccatcgct
    10681 gctgcaatcc gacccgccac cgcctggaac tgaagtgcta ggtcgtccgc gatctgcacg
    10741 ttctgcttca cctgttgcat gcgccgcgac atatcacggt gcccgcggag caccgccatg
    10801 aaaacgcgct gacagggtgc ttacggcaac acgctggtaa cagcttgcgc caaacgctgc
    10861 cgatctggcg ccacttcaga aagccagtcc aagaggcccg gcgggaggcg ttcgatcgcg
    10921 agctcgtaac cctggatgat ccggatgcgg cggatgcgga gctccttgcg gcgcgcgttc
    10981 tcctgctcga gccagtcgag gatgaccggc agcgcttcga cagtggcctt cttgatgtcg
    11041 tgcatcagca ggacgttgcg gccctggatc ttgccgagca tcttctcgag gtagtcgatc
    11101 gtcttcttcg tgctgccgtg cttccactct ttgggatcga ggtcccagtg gaagtgcgtg
    11161 atgccgcgcg cggccagcat cgtgtcgagc agctcgcagc gcacgccgta cggtgcgcga
    11221 aaccagacca tctgcatctt cgcagcggct tcgatgatct tctggccctt gtcgatctcg
    11281 tcgttcgcgc gctgatcgtc tttgagatga cacaggtcct gatgcgtcat cgtgtgattc
    11341 gcgatgatgt gaccgtccgc caagatgcga tcgacgatcg cctgcgcctc gggcgcctcg
    11401 agccgcgcac cgaccatgaa gaagatggcg tggatgtgat gtgcggccag cgcgtcgagc
    11461 acgaacggcg tgcgcttcgg atcaggccca tcgtcgaacg tgaagatgag ctcgggatcc
    11521 cccgtgagcg tcgggcccgc tgcaggacct gaccacgcca gtggcggcgg cttgggaacc
    11581 ttgggcgtgc ccttcgcgtc agccgtgctg acgagcgcag cgaagagcgg gaggaaaagc
    11641 gccctcacgt acttcgatct aacacgcagc acgccggaag cattcttttg agcctatcct
    11701 cgcgaccatg ctcgagcgcc tcgagaacaa agaaccgcct cgcatccgcc tcgccaacac
    11761 gccgacccgt ggccactggc tgcgttatgg cgagcagctg ggtaccaaga tctggctaaa
    11821 gcgcgatgac cacaccggca gcgagctgat gggcaacaag gtccgcaagc tcgagtacct
    11881 gatggccgaa gcgctgcagc tgcaggcgac gcacgtcatc acgtgtggcg gcgagcagag
    11941 caaccacgct cgcgcgaccg cgatggccgc ggcccacctg ggcctcaaga gtgtgctcgt
    12001 cctgcggacc gacacgcccg agaagccgcc ggcgacgacc ggcaacatcc tgctcgatcg
    12061 cctcgtcggt gccgagattc gctggatctc gcgcgccgct tggaaggacc gcaatcgcct
    12121 gctcgccgaa gaagccgagc gcgtgaagag cgccggcggg cgcccgtaca tcattcccga
    12181 aggcggctcc aatgcgctcg gcagctgggg ctacctccgc gcgatgcgcg agctcgtcga
    12241 ggatctcgcg ggcatcgcgg cgccggacaa cccggtgacg gtcgtctacg cgtgcggctc
    12301 gggcggcact ggcgcgggcc tcatcgtcgg ctggaagctg ctcgggcttc aggcactcgg
    12361 tattcgcgtc accggtatcg cggtctgcga tgacaagccg tacttcgtcg attcgatcgg
    12421 caagatctgc ggcgacttcg aagagcgctg gcagctcgac gcgcacgtca cgccgcagga
    12481 catcgacatc atcgatagtc acgtcggcct cggctacgcg aagagccgcc ccgaggagct
    12541 cgcgaccatc cgcgatgtct gccgcagcga cggcatcgtg ctcgatcccg tctatacggg
    12601 caaagcgttc cacggcgtcg tcaccgagct gaagaaggac cccaagcgct tcggcgccgc
    12661 ggtcgcgttc attcacaccg gcggcatgtt cgggctgttc gcttcgcccg accaaatcgg
    12721 ctcggtgctc taaatcgccg cgaagaacac gccgaggccg acgaccaggc tcgtgtcgta
    12781 aggctcgcga gcctcgccga acggacccat gatcgtcgga cgatcgtttg cgtgctggac
    12841 gcggaaccag atggtgccgc ccatgtcgct gcgcagaacg tcgatgcccg caccggcgcg
    12901 cccgctgaag cgcgagaacg acgagtgcgg aattccaggc tcttcgagca tccacatctc
    12961 gcgtgcaatc cccagctcga gccagggacg cagcgaggtg cagctgcgcc gatcgcagat
    13021 gcgcttcggc gacgaaacgg gagaaatgcg gccgccgacg gagagccaat tcgaggagct
    13081 gtcccaggcc caaggctggt gcccaacttc tgcggtaact cccaccggtc cgctccagct
    13141 cgcgcccgaa agcgcgatgc cggggtgacc tgccgaacgc acgtcgctgc tctcactcgc
    13201 tacggccagt gtagggccga gctcgagatt gccgtcggcg tgcgcgaagg caggaaccga
    13261 aacgactgca aggacgagaa gcggcctgaa catgcggcgc gtataccgca agtcaggcga
    13321 gcttcgcgac tacttacaga cggcccgtga tcgcgaggat cacgaggatg aggatgataa
    13381 gacccgccgg gccgaggccc gccgcgccga agcggctgta gccgaagcca ccgccgagga
    13441 gtgcaaggag aagcaggacg atgaggatgg tcatcataat gacccttgct agtgcaacga
    13501 agctgccagg tgatctgatg gcgccgtggg cgctcaccgg gtcagcggag cgctcggcgc
    13561 atcaccgagc tcacatctcg aatggcggaa tcacgcggcc atcgacgtcc gtgaacccgt
    13621 acctagcggc aagctcaccg acgtagagca cctcgccggt tttctgatgc gcgtgcggat
    13681 cagatgcgag cgcgaccact gcgcgcccta gataccgtgg cgattcggtg cgcgcgagcg
    13741 gcggcacatc tgtccagccc cgctcatcgg tcgaaaacgc tgcgaggatg atttcggttc
    13801 gcatccagcc aggcacgagc gccacggatg tcacttcatg cgcgcgcagc tcctcggcga
    13861 gagcgaatgc aaatcggttc atcgtggctt ttgcgacgtc gtaaaacaga ttccctttga
    13921 tgtaacggcc gcggtcccaa aagctcgtcg tgatgatgag tcctcgtcgc tggcgcacga
    13981 acaacggggc agcagcgtgc gatgcgacga cgtggttgcg cacaccgtgg tcgaacatcg
    14041 attcccagtg atcgatcgac agctcccaaa atggtgcgga caattgatcg gcggtgagcg
    14101 tctcgtgccc gccccacgcg ttgttgacga gtaggtcgag ctggccttgc tcgcggtcga
    14161 ctcgtgcgaa cagctcgcgc acttgtgcaa cgtcggagtg atcgcagcgc accgcgatgc
    14221 cgcgaccacc ggcggccgtc acttcttccg cgacgtcttc gatcgtgccg ggcatcttct
    14281 cgaggtgccc ttgcgcgagc attcgctcgt acgccggtgg cgcgtgcgaa cggctcgtgc
    14341 gaccggtgac gtagacagtg gcgccggcgg ctccgagctc gaccgcgacg ccgcgtccgc
    14401 cgcctcgact cgcgcctgtc acgaccgcga tcttcccctg gagccttgtc attccgcgca
    14461 tcttgccacg tctgttaaaa cggcaaagca tccgtattga cagaccgtcc gctataacgg
    14521 acagtcgtcc acatgacacg ggtcctcctc ctgtctctcg tccttgctgc ttgctccacc
    14581 gagccgaaga cgacgtcggc gagcaaggaa gtgaagctcc tcaacgtttc gtacgacccg
    14641 actcgcgagc tctacgccga catcaatgcg gcgtttgcga agcagtggga ggcgaagacc
    14701 ggccagcacg tgacgatcga gcaatcgcac ggcggctccg gcaaacaagc acgcgcggtg
    14761 atcgacggcc tcgaagccga tgtcgtcacg ctcgccctcg catacgacgt cgatgcgatc
    14821 gcgaaagcgg gcctcatcaa acccgactgg cagacgaagc tgccggagag ctcgacgccg
    14881 ttcacgtcga cgatcgtgtt cctcgtccgc aagggcaatc cgaaaggcat ccacgactgg
    14941 gccgacctcg tgaagccagg cgtcgaagtg atcacgccga acccgaagac gtcgggcggc
    15001 gcgcgctgga actacctcgc ggcgtggggc tacacgctgc agaccggtgg cgacgaggcg
    15061 aaggcgaagg cgttcgtcgc ggatctctac aagcatgtcc ccgtgctcga cacgggcgcg
    15121 cgcggtgcga cgacgacgtt tgccgagcgc ggcatcggcg acgtgctcat cgcctgggag
    15181 aacgaggcct atctcgcgat caacaagctc ggcgccgaca aggtcgagat cgtcgtgccg
    15241 agcgtgtcga tcctcgcgga gccgcccgtc gccgtcgtcg acgatgtcgt ggacaagcga
    15301 ggcacgcgcg aggtcgcgac cgcgtacctg acgttcctct acagccccga ggcgcagcag
    15361 atcgcggcga agcagttcta tcgcccgcgc gacacgacgg tccagacatc gttcgccgac
    15421 gtgaagctgt tcacgatcga tcaggtgttc ggcggctggg cgaaggcgca gcccgcgcac
    15481 ttcaacgacg gcggccagtt cgacgcgatc acggcgaagt aaatggcacg acgttccgcc
    15541 atcccggggt tcggtctgac gatgggctac acgatcgtgt acctgtcgct gatcgtcctc
    15601 atcccgcttg cgacgctcgc gatcaagacc gcgcagctgt caccgagcga gatctgggag
    15661 ctggcgacga gcaagcgcgc gattgcttcg tacaagatca cgttcggcat ttcgctcctc
    15721 gccgcgctgc tcaacgtcgt gttcggcagc ctgctcgcgt ggacgctggt gcgctaccgc
    15781 ttcttcggca agagcatcct cgatgcgatc gtcgacattc cgttcgcatt gccgacggca
    15841 gtatcgggcc tcgcactcac ggcggtgctc gcaccgaatg gctggatcgg tcgcttcctc
    15901 gaaccgctcg gcattcgcgc cgtgttcaac ccgctcggcg tgctgatcgc gctcatgttc
    15961 atctcgctgc cgttcgtcgt gcgcacgatc cagccggtgc tcgaggagat cgaacccgaa
    16021 gtggaagagg cggcggcgac acttggcgcg acgcgctggc agactctcta ccgcgtcatc
    16081 ctgccgggct tgtggcccgc gatggtcacg ggcttcgcgc tcgcgttcgc acgggcgatc
    16141 ggcgagtacg gctcggtcgt cttcatctcc ggcaacaagc agtactcgac ggagatcacg
    16201 ccgtacctca tcatgacgaa gctcgacgag ttcgattttg cgtcggcgac ggcgctcgcg
    16261 ctcgtgatgc tcgccgtgtc cttcgcgatg ctgctcgcga tcaacggctt ctcggcgtgg
    16321 gtgcgccggc ggcagggagc attcgcgtaa tggtgacgtc agcgccaccg attgccgaac
    16381 gtgtcgtcgc cgcgtcgacc agcgagcccg tgtgggcgcg ccgcatcctc atcggcatct
    16441 ccgtcgggtt ctccgcgctg ttcatcctcg tgccgctcgc cgcgatcttc ggcgaagcgt
    16501 tctcgcgcgg ccttcgcgcg ttcggccaag cgctcgtcga tccgaaggcg ctctcggcga
    16561 tcaagctgac gctgatcacg accgcgatcg tggtgccact caatctcgtg ttcggcgtgt
    16621 ccgccgcatg ggcgatcgcg cggttccggt tctggggccg ctcgttcctg atctcgctga
    16681 tcgatctacc gcttgccgtt tcgccggtga tcgcgggcat ggtgttcgtg ctgctgttcg
    16741 gctcgcgcgg cggcttcctc ggcccatggt tcgcggcgca cggcgtgaag atcatcttcg
    16801 gtctaccggg catcgtgctc gcgacgacgt tcgtgacgtt cccgttcatc gcccgcgagc
    16861 tcatcccgct gatggaggcc cagggcagcg acgaggaaca ggccgcggtc tcgctggggg
    16921 ccggcgggtg gacgaccttc cggcgcatca ccctccccaa catcaagtgg ggcctgctgt
    16981 acggcgtcct cctctgctcc gcccgcgcgc ttggggagtt cggcgcggtc gccgtcgtcg
    17041 ccgg
//
LOCUS       DATLBZ010000494         3157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2878914, whole genome shotgun sequence.
ACCESSION   DATLBZ010000494 DATLBZ010000000
VERSION     DATLBZ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2878914"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..2384
                     /locus_tag="VMZ53_24990"
     CDS             33..2384
                     /locus_tag="VMZ53_24990"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HUS31792.1"
                     /translation="MSAGVGGGPSVLVGDPVVFAEAMKRKRNAILCGALLAACGGSPK
                     TAPVELGTTHSSTDVMPATPAVVEAVDPNLAFRLSYSNPGGMWMPAQMTLPQHVETFR
                     KMGVKLPAEALANPLSETLTSIVSLGGCSASFVSPDGLVVTNHHCVQGALKYLSTKEN
                     NLIENGFLAKTREEELAAGPDKRVMVVQAYKDITHEMRDGLEAIKDPIARKDEKEKRY
                     KQQIAACEKDRPWLRCSVSGFFGGGQYQLIEMLEIKDVRLVYVPHRAIGNYGGEIDNW
                     AWPRHTGDWSFYRAYVGKDGKPAEYSPDNVPFKPQHWLKVSTAGLRAGDFVMVTGYPG
                     ETSRTETAAAIHHEVEWYLPYAIAAMQEKYKMLESHLDDGNETALAATTAKQGTQNYL
                     EKYQDMLEGLTKGDLLRQKDDIDRRVKEWAALPGHEVHKAAIEKMEKIQSEEFRTARL
                     DYDRGAALRGSKLLATSTSFTRWAEERAKKNEERKPGYQERDMPRALASQKSFSRSFD
                     RTLDREGFRLTLVRALQLPEQDRTWLATLLGAKKGQKIDEALIDKTLDAWYKAPTLED
                     VDVRLDLLQNGTSKQLKASKDPFIKAAQRIWPLIKAEEKKDNAKAGDLMLVEPYYADA
                     MRQVLGGVLPPDANGTLRITYGTVKPVKSDESAFTTASQIPAKDKGEEPFDAPKAELE
                     AIKAKRYGRYADPSLGNELPVDFLSDLDITNGSSGSATLNDKGELVGLVFDGNKEGVA
                     SDVVFNPSVTRSIHVDARYMIWVMDALDNADHLIKEMGLQPQL"
     gene            complement(2353..>3157)
                     /locus_tag="VMZ53_24995"
     CDS             complement(2353..>3157)
                     /locus_tag="VMZ53_24995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HUS31793.1"
                     /translation="DAMELLKKLPDGSVDLVVTDPPYAIAKEDWDEFESLDVYVDWCD
                     TWLAEVARVLAPHGSAYVCGFSEILADVKARSAKRFAGCRWLVWYYKNKANLGRDWGR
                     SHESILHLRGEDATLDVDSVRVPYNGHTTKYPARVQAVNSQYGRGVRRDKWEPHPLGA
                     KPRDVIEVPVICNGMTEKTPHATQKPEALIEKLVLASSAAGQLVVDPFVGSGTTAVVA
                     AKLGRRWLAGDADARYVGLARERVTNATADTSVAKAKRATRAAAADPSL"
BASE COUNT          606 a         1023 c         1027 g          501 t
ORIGIN      
        1 cgcagggtgt cctgtgacct gtggtctcta ccgtgtcagc gggagtcggc gggggaccca
       61 gcgtcctcgt cggcgatccc gtggtatttg ccgaggcgat gaagcgcaag cgcaatgcca
      121 tcctgtgtgg agccctgctc gctgcctgcg gcggatctcc caagaccgcg ccggtcgagc
      181 tcggcacgac gcattcgtcc acagacgtca tgcccgcgac gcccgctgtc gtcgaggcgg
      241 tggatccgaa tctcgcgttc cggctttcgt actcgaaccc gggcggcatg tggatgccgg
      301 cgcagatgac actgccgcag cacgtcgaga cgttccgcaa gatgggcgtg aagctgcccg
      361 ccgaagcact cgcgaatccg ctcagcgaga cgcttacgtc gatcgtgtcg ctcggcggct
      421 gcagcgcatc gttcgtatcg cccgacggtc tcgtcgtgac gaaccatcac tgcgtgcaag
      481 gcgcgctgaa gtatctctcg acgaaagaga acaacctgat cgagaacggc tttctcgcga
      541 aaacgcgcga agaggagcta gcggccggtc cggacaagcg cgtgatggtc gtgcaggcgt
      601 acaaggacat cacgcacgag atgcgcgacg gcctcgaagc gatcaaggat ccgatcgcgc
      661 gcaaggacga gaaagagaag cgctacaagc agcagatcgc ggcgtgcgaa aaagatcgcc
      721 cgtggctgcg ctgctcggtg tcaggtttct tcggcggcgg tcagtaccag ctgatcgaga
      781 tgctcgagat caaggacgtg cgtctcgtct acgtgccgca tcgcgcgatc ggcaactacg
      841 gcggagagat cgacaactgg gcgtggccgc gtcacaccgg tgactggtcg ttctatcgtg
      901 cgtacgtcgg caaggatggc aagcccgccg agtactcgcc cgacaacgtg ccgttcaagc
      961 cgcagcactg gctgaaggtc tcgacggcgg ggctgcgggc cggcgacttc gtgatggtga
     1021 ccggctatcc gggcgagacg agccgcaccg agaccgccgc tgcgatccat cacgaggtgg
     1081 agtggtacct gccatacgcg atcgccgcga tgcaggagaa gtacaagatg ctcgagtcgc
     1141 acctcgacga cggcaacgag actgcgctcg ccgcgacgac cgcgaagcag ggcacgcaga
     1201 actacctcga gaagtatcag gacatgctcg agggcctgac gaagggcgac ctgcttcgtc
     1261 agaaggacga catcgatcgt cgcgtgaagg agtgggcggc gctgcccggc cacgaggtgc
     1321 acaaggccgc gatcgagaag atggagaaga tccagagcga ggaattccgc acggcgcgcc
     1381 tcgattacga tcgcggtgca gcgctccgcg gcagcaagct gctcgcgacg tcgacgtcgt
     1441 tcacgcggtg ggccgaagag cgcgcgaaga agaacgagga gcgcaagccc ggctaccagg
     1501 agcgcgacat gccgcgcgcg ctcgcgagtc agaagtcgtt ctcgcgctcg ttcgaccgca
     1561 cgctcgatcg cgaaggcttc cgcctcacgc tggttcgcgc gctgcagctg ccggagcaag
     1621 atcgcacgtg gctcgcgacg ctcctcggtg ccaagaaggg ccagaagatc gacgaagcgc
     1681 tcatcgataa gacgctcgac gcttggtaca aggcgccgac gctcgaagac gtggacgtcc
     1741 gcctcgacct gctgcagaac gggacatcga agcagctgaa ggcctcgaag gatccgttca
     1801 tcaaggcagc gcagcggatc tggccgctga tcaaagccga agaaaagaag gacaacgcga
     1861 aggccggcga cttgatgctc gtcgagccgt actacgccga tgcgatgcgc caagtcctcg
     1921 gcggtgtgct tccgcccgat gcgaacggca cgctgcgcat cacgtacggc acggtgaagc
     1981 cggtgaagtc cgacgagtcc gcgttcacga ccgcgtcgca gattcccgcg aaggacaagg
     2041 gcgaggagcc gttcgacgcg ccgaaggccg agctcgaagc gatcaaggcg aagcgctacg
     2101 gccgctacgc cgatccgtca ctcgggaacg agctgccggt cgacttcctc tcggatctcg
     2161 acatcacgaa cggcagctcg ggctcggcaa cactcaacga caagggcgag ctcgtcggtc
     2221 tcgtcttcga cggcaacaaa gaaggcgtgg cttcggacgt cgtgttcaac ccgtcggtga
     2281 cgcgctcgat tcacgtcgac gcgcgctaca tgatctgggt gatggacgcg ctcgacaacg
     2341 ccgaccatct catcaaagag atgggtctgc agccgcagct ctagtcgccc gcttcgcctt
     2401 cgcgacgctc gtatcggccg tcgcattcgt cacgcgctcg cgagccagac cgacgtagcg
     2461 cgcatcggcg tcgccggcga gccatcgccg accgagcttc gccgcgacca cggccgtcgt
     2521 tccgctgccg acgaacggat cgacgacgag ctggccggcc gcgctcgacg caagcacgag
     2581 cttctcgatc agcgcttcgg gcttttgcgt cgcgtgcggc gtcttctccg tcatgccgtt
     2641 gcagatgacg ggcacctcga tcacgtcgcg cggtttcgcg ccgagcggat gcggctccca
     2701 cttgtcgcgc ctcactccgc ggccgtactg cgagttcacc gcctgcacgc gcgctggata
     2761 cttcgtcgtg tggccgttgt acggcacgcg cacgctatcg acgtcgagcg tcgcgtcctc
     2821 gccgcgcaga tgcagaatcg actcgtgcga gcgaccccag tcgcggccga ggttcgcttt
     2881 gttcttgtag taccagacga gccagcgaca ccccgcaaac cgcttcgcgc tgcgcgcctt
     2941 cacgtcggcc aagatctccg agaagccaca cacgtaggcc gagccatgcg gtgcgagcac
     3001 gcgcgcgact tcagcgagcc acgtgtcaca ccaatccacg tacacatcga gggactcgaa
     3061 ctcgtcccag tcttccttcg cgatcgcgta cggcggatcc gtgacgacga ggtcgacgct
     3121 gccgtcgggg agcttcttca agagctccat cgcgtcg
//
LOCUS       DATLBZ010000495         8368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1388158, whole genome shotgun sequence.
ACCESSION   DATLBZ010000495 DATLBZ010000000
VERSION     DATLBZ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388158"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..387)
                     /locus_tag="VMZ53_25000"
     tRNA            complement(306..387)
                     /locus_tag="VMZ53_25000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(351..353),aa:Leu,seq:gag)
     gene            478..1071
                     /locus_tag="VMZ53_25005"
     CDS             478..1071
                     /locus_tag="VMZ53_25005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HUS31794.1"
                     /translation="MSLYQGSPPPPLSRATLVIGLYTAMGIVAFLLAAGRGDPDVYRL
                     GGNPEGWQLASAPVFGVVLGLAVVGLTRVATSRFQWARDLHTSFKDLLGPLTGREIVI
                     LALASSIGEELLFRGALLPWFGVWISAAVFAALHIGPGRRFLPWTLSALVLGVAFGLL
                     AKWTGNIGGPIAAHFTINFLNLKYIVGAPARRAAPAV"
     gene            complement(1076..1639)
                     /gene="msrA"
                     /locus_tag="VMZ53_25010"
     CDS             complement(1076..1639)
                     /gene="msrA"
                     /locus_tag="VMZ53_25010"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HUS31795.1"
                     /translation="MRNVLLVAAVLAASSSADADPAPKREVAIVAGGCFWGMEHVMRD
                     LPGVISMDVGYAGGKSTSVGYHDVSEGTTGHAESVRIVFDPSKISYDDILLRFFRGHD
                     PTQVDRQNNDIGTQYRSEIFTTSPTQYATALAIRTRVENSHQWKKPVVTKIEPATTWV
                     QAEDYHQDYLVKHPNGYNDHFLRPYKF"
     gene            2036..2233
                     /locus_tag="VMZ53_25015"
     CDS             2036..2233
                     /locus_tag="VMZ53_25015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31796.1"
                     /translation="MVEGTVVGEEEEAFAVVVEAAGDVYARRQTELRERWATFRVREL
                     RVDEVRLVESDQQQGDQPRFE"
     gene            complement(2215..2937)
                     /locus_tag="VMZ53_25020"
     CDS             complement(2215..2937)
                     /locus_tag="VMZ53_25020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HUS31797.1"
                     /translation="MGRAFEKRKHTIFARMDRVAKQFTKINKDIAIAVKASGPSPDAN
                     PALRRALQNARALNMPKDKVEAAIKKASGQGAADYEIVIYEGYAPHGIALLVETATDN
                     VTRTVANVRHAFKANGGNMGTTGSVARMFEKQGVFRLNPEGIDRDALELELIDHGLTA
                     LEDGESDKGEKQLVVRCAFSDFGAMQTALEARKATVISSSHEFVATTLTELPEDKADE
                     VMKLVGTLEDDDDVQNVYSNLG"
     gene            complement(2952..5168)
                     /locus_tag="VMZ53_25025"
     CDS             complement(2952..5168)
                     /locus_tag="VMZ53_25025"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31798.1"
                     /translation="MRGLAVLVLALGACGRLNFDDLRAGSGSDADSDGGAFVQSCFRA
                     IASGNDFTCVIDARGDVWCWGSNRFFQSDPTQAGPEAILLPRKVSLPRPAVQLALGNS
                     VACARLDDGHARCWGSDFWGSLGDGGGMTGPGPIEPAIGDTIVDIAAGGETVCALRST
                     DRRLLCWGKNTLFEAARPTGMSIDTPTLIDNTTGTKELALGHHHGCLIDASGVFKCWG
                     RAGSSEIGDPTVDTPTPRTPPAYAAITHLVRGGGRFTCAIETSGDLRCVGNNNSLQLG
                     RDDTYPSSTPLAPIVSGAASASLGSMSGCALFPDHHVECWGDGTDGLLGPTHLEPSAT
                     AVRVLDNVSALESGAHHACALRGPDVLCWGYNFLGELGRGDISVRASPTHVGSLPSAT
                     DLAVGPEHACMRSGLQLYCWGSNQQGQLGDGTRLSRPVAAAVDAGIAVDGVAVGAMNT
                     CVWGGGIVRCAGRADSGQTGDTTYPPQSLAFRTVAGATAVSFVDIGTGQICGIDGGDV
                     KCWGGNAYGELGTGDTMARATPTAIGLSNAIDVSAGTASTCAVAGGTLSCWGDNGHHQ
                     VSPLNTMRFLTPTAVNIGTDTPAQVSVGFTHACVLTTTGTIKCWGGNDAGEVGNNTIV
                     DQLNPVDVSLPMAAAEVRAGSKLTCARLVDGTVYCWGLNEHGELGTGNFTWASVPIQV
                     PNITDALAIEVGDGYACARHATGTIECWGNERRGRLGTGRLTLDATPTPVALSCMP"
     gene            complement(5185..6312)
                     /locus_tag="VMZ53_25030"
     CDS             complement(5185..6312)
                     /locus_tag="VMZ53_25030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31799.1"
                     /translation="MRGGVVFGLLVGCYSPTPHAGAPCPDGVCPTGLVCSPATMTCEI
                     SATPKADAAVDAEPDAFVPVDANVSLFAYRRRITIVNNSSTTMPTGFSIRVPLGNLAT
                     LVSQGKVNADFSDLRVIGDTLGERDRIVDPPSGPAPPALTFSLASSIAAGATSSEYAL
                     YYGRPTAPAAPANGNNVFPLFDEFNAGLSSVWLQNDAPSVSGGKVTLRANRLDALATN
                     AATDNVPLVCSLEMITTISDPNSDPTVQPTGTLYYWFGFQRTGDFQAIDPWSVWIARG
                     KGQLHAEQKSPNGCDPTSCDGPYATQDTTPHYYVVERDTGATRFYRDGTLSYTATVTN
                     TDMSPMLRNFTAASTVTIDYVRARARVSPDPTVTVGNEQAL"
     gene            6395..7123
                     /locus_tag="VMZ53_25035"
     CDS             6395..7123
                     /locus_tag="VMZ53_25035"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS31800.1"
                     /translation="MRGRLVLVTGGSRGIGRAIVDECLALGADVIAVARDASAIAGEH
                     VTGLAADVTTGEGRARIVDAVRERGALHVLVHNAGTNVRGPLISYDDATIARLLDLNL
                     TSPLLLSRDLHPFLRAANGASVVHIGSVAGHVALPTGVAYAAAKAGLAQATRTLALEW
                     ARDAIRVNLVAPWYTRTPLVEPVLAKPEALAAIIARTPMGRIAEPREVATAVAFLAMP
                     AASYITGQSLAVDGGMTIQGLPVA"
     gene            7131..8090
                     /locus_tag="VMZ53_25040"
     CDS             7131..8090
                     /locus_tag="VMZ53_25040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31801.1"
                     /translation="MRLGRYDVDELLAKGGMGEVYKGRLVAEHGFVRPIVIKLIRTEL
                     LADERAALMFIDEARMAAGLHHRNILQIVDFDRFQGGAFIVTEWIDGCDLRTLLKEMR
                     AAPRYDIALTIISELATGLDAAHDATGADGAPLHLVHRDVSPSNVLLGKQGEIKLSDF
                     GIAKARMRSYHTVSGTIKGKAPYMAPEQILGEVLDRRADIFSLGVLLFEVTTRTRLYS
                     GTGDANAMKMILAGDVPDPAERRPGFPPELTQIIRKALARDRNRRYSTAGELADDLDE
                     VARSRKWSLSCTAVGEVARAIAARAGQSTPTPGAGRLSLGGQP"
     gene            8087..>8368
                     /locus_tag="VMZ53_25045"
     CDS             8087..>8368
                     /locus_tag="VMZ53_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31802.1"
                     /translation="MNPGAAESAHDRVGPYQLVKAIGAGGSARVDLARIDRAYGFQRH
                     VVIKRPLEHLRSDQKAGDSLQREARLGGRLRHPNLVSVLDAGRHDGYDYL"
BASE COUNT         1317 a         2553 c         2933 g         1565 t
ORIGIN      
        1 cgagccgcgc cggggccggc ggcgatgtcg cggcgcgcgc cctggccgtt gaaaggccgg
       61 gcgcgccgct caccgccggc ccggcgctct gagcggcgtg gcggagagag ccgtcgaact
      121 cgtcgtacga caaagtcatc gtcgcgcacg aaggggtcgt aatgaccagt caccagcgct
      181 ctagttggga ctcgaacccg gtaacaccga accggtgaca ccgaaaacga aaacagcggc
      241 atttctgccg cttagttcgt gcgcgtcgcc agtcgggact caaacccgct actggctcag
      301 cgtcgtgcgc gaggggggac tcgaaccccc acacggttaa gtactagcac ctcaagctag
      361 cgcgtctacc aattccgcca cccgcgcaat tcagttgtga atgaagggct gtttgagtac
      421 cgctcgacat cgcctctgtc aaggtggccc ggttctgcat agtattcgtc cgtcgccatg
      481 tcgctgtacc aggggagccc gccaccgccg ctatcgcgcg cgaccttggt gattgggctt
      541 tatacggcca tgggcatcgt cgcgtttctg ttggcggcgg ggcgtgggga tccggatgtg
      601 tatcggttgg gcggtaaccc ggaggggtgg cagctagcga gcgcgccggt ttttggcgtc
      661 gtgttggggt tggcggttgt ggggctgacg cgggttgcga cgagccgctt tcagtgggcg
      721 cgcgaccttc acacgagctt caaggatttg ctcgggcctt tgacggggcg cgagatcgtg
      781 attttggcgt tggcgagctc gattggggag gagctgctgt ttcgcggggc gttgttgccg
      841 tggtttggcg tgtggatttc cgcggcggtg tttgcggcac tgcacattgg gccggggcgg
      901 cggtttctgc cgtggacctt gagtgcgttg gtgctgggcg ttgcgttcgg gttgttggcg
      961 aagtggaccg ggaacattgg cgggcctatt gccgcgcact tcacgatcaa ctttttgaat
     1021 ttgaagtaca tcgtcggtgc gccggcacgc cgcgctgcac ctgccgtcta ggcggctaga
     1081 atttgtacgg ccgcaggaag tggtcgttgt agccgttggg gtgtttgacc aggtagtcct
     1141 ggtgatagtc ctcggcttgg acccaggtcg tcgccggttc gatcttggtg acgacgggct
     1201 tcttccattg atgcgagttt tcgacgcgcg tccggatcgc gagagcggtc gcgtattgcg
     1261 tcggtgatgt cgtgaagatt tcgctgcggt attgcgtgcc gatgtcattg ttctggcgat
     1321 cgacttgcgt cgggtcgtgg ccgcggaaga agcgcagcag gatgtcgtcg tacgagatct
     1381 tggatgggtc gaacacgatg cggacggatt cggcgtggcc ggtcgtgcct tcgctgacgt
     1441 cgtggtagcc gacgctcgtc gacttgccac ctgcgtaacc gacgtccatc gagatgacgc
     1501 ccgggagatc gcgcatcacg tgctccatgc cccagaagca gccgcctgcg acgatcgcga
     1561 cttcgcgttt cggtgcggga tcggcgtcgg cggaggagga agcagcgagc acggcggcga
     1621 cgaggaggac gtttcgcatg tcctctttac gtcagcggcg tggggcggtt acaggcgtga
     1681 tctcgcgcca ttgacctgga gcgaggccgt cgagggcgta ggggcctatc gtgatgcgga
     1741 tgaggcgaag cgtggggagg ccgaccgcgg ccgtcatccg gcggacttgg cggttgcgtc
     1801 cttctttgat cgtgagtgcg agccacgcgt cggggacgga tttgcggaag cggatcggcg
     1861 gggtgcgcgg ccagagccac ggtggggctt cgattgcgtg ggcttcggcg gggagcgtgg
     1921 ggccgtcttt tagatcgatg ccggtggcga gacggcgaag agcgtcgtcg gtgatcgtgc
     1981 cttcgacttg ggcgaggtag gtcttcggcg ccttccactt cggatcggtg aggcggtggt
     2041 tgagggaacc gtcgtcggtg aggaggagga ggccttcgct gtcgtggtcg aggcggccgg
     2101 cgacgtttac gcgcggcggc agaccgaact gcgcgagcgt tgggcgacct tccgcgtccg
     2161 tgaacttcga gtggacgagg tacggcttgt tgagagcgat cagcagcagg gcgatcagcc
     2221 gagattcgag tagacgttct ggacgtcgtc gtcgtcttcg agcgtgccga cgagcttcat
     2281 gacttcgtct gctttgtctt cggggagctc cgtcagcgtg gtcgcgacga attcgtgcga
     2341 cgaggagatg acggtggctt tgcgagcttc gagcgcggtt tgcatcgcgc cgaagtcgga
     2401 gaacgcgcag cgcacgacga gctgcttttc gcctttgtcg ctctcgccgt cttcgagcgc
     2461 ggtgaggccg tgatcgatca gctcgagctc gagtgcgtcg cggtcgatac cttcggggtt
     2521 gagccggaag acgccttgct tttcgaacat gcgtgcgacc gagccggtcg tgcccatgtt
     2581 gccgccgttg gctttgaatg cgtggcggac gttggcgacc gtgcgcgtga cgttgtcggt
     2641 tgccgtttcg acgaggagcg cgatgccgtg cggtgcgtag ccttcgtaga tgacgatttc
     2701 gtagtctgct gcgccttggc cgctggcctt tttgatcgcg gcttcgactt tgtctttcgg
     2761 catgttcaac gcgcgagcgt tttgcagcgc gcggcgaagg gcggggttgg cgtcgggact
     2821 cgggccgctc gctttgacgg cgatcgcgat gtccttgttg attttggtga actgcttcgc
     2881 gacgcgatcc atgcgtgcaa agatcgtgtg cttgcgtttc tcgaatgcgc gtcccacggc
     2941 gggacgtagt actacggcat gcaggaaagc gctacaggcg tcggcgtggc gtcgagggtg
     3001 aggcggccgg tgccgaggcg gcctcttcgt tcgttgcccc agcactcgat cgtacccgtg
     3061 gcgtgtcgcg cacatgcata gccgtcgccg acctcgatcg cgagcgcgtc ggtgatgttt
     3121 ggaacctgga tgggcacgct ggcccacgtg aagttgcccg tgccgagctc gccgtgctcg
     3181 ttgaggcccc agcagtagac cgtgccgtcg acgagacgtg cgcacgtcag cttcgagcct
     3241 gcgcgaactt cagcggcggc catcggcagc gagacatcga cagggtttag ttggtcgacg
     3301 atcgtgttgt tgccgacttc gccggcgtcg ttgccgcccc aacacttgat cgtgccggtc
     3361 gtcgtgagca cgcacgcgtg tgtgaagccg acgctgacct gcgccggtgt atcggtgccg
     3421 atgttgacgg cggtcggcgt gagaaaccgc atcgtgttga gcggcgagac ttggtgatgg
     3481 ccgttgtcac cccagcagct caaggtgcct ccagcgactg cgcacgtcga agctgttccg
     3541 gctgagacat cgatcgcatt cgagagtccg atcgccgtcg gcgttgcgcg ggccatcgtg
     3601 tcgccggtgc cgagctcgcc gtacgcgttg ccgccccaac acttcacgtc gccgccatcg
     3661 atgccgcaga tctgccccgt gccgatgtcg acgaaggaga cggccgttgc gcccgccacc
     3721 gtgcggaatg cgagtgattg cggagggtac gtcgtgtcgc cggtttggcc gctgtccgcg
     3781 cgacccgcgc agcgcacgat gccgccgccc cacacgcacg tgttcatcgc gccgacggcg
     3841 actccatcaa ctgcgatccc ggcatcgacg gccgccgcga cgggacgaga gaggcgcgtt
     3901 ccatcgccga gctgtccttg ttggtttgag ccccaacaat agagctgcaa gcctgatcgc
     3961 atacacgcgt gctctgggcc gactgcgaga tccgttgcgg aggggaggct gccgacgtgt
     4021 gtaggcgagg cgcgaacgga gatgtcgccg cgcccgagct cgccgaggaa gttgtagccc
     4081 cagcagagga cgtccggtcc gcggagcgcg cacgcgtgat gtgcaccgct ctcgagggcg
     4141 gacacgttat cgaggacgcg caccgcggtc gcgctcggct cgagatgcgt cggtccgagg
     4201 agaccgtcgg tgccatcgcc ccagcattcg acgtgatgat cggggaacag cgcgcaaccg
     4261 gacatcgagc cgagcgatgc agatgcagcg ccactgacga ttggagcgag cggcgtggac
     4321 gacggatacg tgtcgtcgcg tccgagctgg aggctgttgt tgttgccgac gcagcgcaga
     4381 tcgccgcttg tttcgatcgc gcaggtgaag cgaccgccgc cgcgcacgag atgcgtgatg
     4441 gctgcgtacg ccggcggcgt ccgtggtgtc ggcgtgtcga ccgtgggatc gccgatttcc
     4501 gacgagcctg cgcgacccca gcacttgaag acgccgctcg cgtcgatcag gcagccatgg
     4561 tggtgaccga gcgcgagctc cttcgtgccg gtcgtgttgt cgatgagtgt cggagtgtcg
     4621 atcgacatgc cggtcgggcg cgccgcttcg aacagcgtgt tcttgcccca gcacagcagt
     4681 cgtcgatcag tcgaacgcag cgcgcacacc gtctcgccgc ctgctgcgat gtcgacgatc
     4741 gtgtctccga tcgcgggctc gatgggacct ggacctgtca tgccaccgcc gtcgccgagc
     4801 gatccccaga agtcgctgcc ccaacaccgc gcgtgaccgt cgtcgagacg agcgcatgcg
     4861 accgaattgc cgagggcgag ctgaacggct ggacgcggta gcgagacctt gcgcgggagc
     4921 aagatagctt ctgggccggc ttgcgtgggg tccgactgaa agaagcggtt cgagccccag
     4981 caccagacgt cgccgcgcgc atcgatcacg cacgtgaagt cgttgccaga agcgatcgcg
     5041 cggaaacacg actggacgaa cgcgccgcca tcgctgtctg catcgctgcc actgcctgcg
     5101 cgcagatcat cgaagttcag tcgcccgcat gcgccaagcg cgagcaccaa gacggcgaga
     5161 ccgcgcatcg ctacggcgag tgtgtcacag cgcttgctcg ttgccgacgg ttacggtcgg
     5221 atccggcgac acgcgtgcac gtgcgcgtac gtagtcgatc gtgacagtgc tcgccgccgt
     5281 gaaatttcgc agcatcggag acatgtccgt attcgtgacg gtcgcggtgt acgagagcgt
     5341 gccgtcccga tagaagcgcg tcgcgccggt gtcgcgctcg acgacgtagt agtgcggcgt
     5401 cgtgtcttgc gtcgcgtacg ggccatcgca agacgtcgga tcgcaaccgt tcggcgactt
     5461 ctgctcggcg tgcagctggc ccttgccgcg cgcgatccac accgaccacg gatcgatcgc
     5521 ttgaaagtcg ccggtgcgtt ggaagccgaa ccaatagtag agcgtgccgg tcggctgcac
     5581 cgtcggatcg ctgttcggat cactgatcgt cgtgatcatc tcgagcgaac agacgagcgg
     5641 aacgttgtcg gtcgctgcat tggttgcaag cgcgtcgaga cgattcgcgc gcagtgtgac
     5701 tttgccgccg ctgacgctcg gcgcatcgtt ctggagccac acgctcgaga ggcctgcgtt
     5761 gaactcgtcg aacagtggga acacgttgtt gccgttcgcc ggtgcagcag gggcggtcgg
     5821 ccgaccgtag tagagcgcgt attcgctgct cgtcgcacca gcggcgatcg aagacgcgag
     5881 cgagaacgtg agtgcgggcg gtgccggccc cgacggcgga tcgacgatgc gatcgcgttc
     5941 gccgagcgtg tcgccgatga cgcgcagatc ggagaagtct gcgttgacct tgccctgcga
     6001 gacgagcgtc gcgaggttgc cgagcggaac gcggatcgaa aatcccgtcg gcatcgtcgt
     6061 gctcgagttg ttgacgatcg tgatgcgacg gcgatacgcg aacagcgaca cgtttgcatc
     6121 gacgggcacg aatgcgtcgg gttctgcatc gacggcggca tccgctttcg gcgtcgcgct
     6181 gatctcgcac gtcatcgtcg cgggcgagca gacgagcccg gtcgggcaca caccatccgg
     6241 gcacggcgcg ccagcgtgcg gcgttggcga ataacagcca acgagcaggc cgaacacgac
     6301 gccgccgcgc atgcatcaat catagcccgg cgctgcaacg ccgtctgcat gcctctttgg
     6361 tgcaactatc ccggcgatga cagacgcttg gaccttgcgc ggacggctcg tattggtaac
     6421 cggcggtagc cgaggaatcg ggcgcgcgat cgtcgacgag tgcctggcgc tcggcgcgga
     6481 tgtgatcgca gtcgcgcggg atgcgtcggc gatcgcgggc gagcatgtga cgggcctcgc
     6541 agcggatgtg acaacgggcg aggggagggc gcgaatcgtc gacgcggttc gcgagcgagg
     6601 cgcgctgcac gtgctcgtcc acaatgcggg cacgaacgtg cgtgggccgc tcatcagcta
     6661 cgacgacgcg acgatcgcgc gcttgctcga cctcaacttg acgtcgccgc tgctgctgtc
     6721 gcgtgatctg catccatttc tgcgcgcggc gaatggcgcg agtgtcgtgc acatcggatc
     6781 ggtcgccggc catgtcgcgc tacccaccgg ggtcgcctac gccgcggcga aagcggggct
     6841 agcgcaggcc acgcggacgc tcgcactcga atgggcgcgc gatgcgattc gcgtgaacct
     6901 cgtcgcgcct tggtatacgc gcacgccgct cgtcgagccg gtgctcgcaa agcccgaggc
     6961 attggccgcg atcattgcgc gcacgccgat gggcaggatc gccgaaccgc gcgaggttgc
     7021 gacggcggtg gcgtttctcg cgatgcccgc tgcgtcgtac atcacggggc agagcctcgc
     7081 cgtcgacggt ggcatgacca ttcaaggctt gcccgtggca tgatcggacc gtgcgtctcg
     7141 gtcggtatga cgtcgacgag ctgctcgcaa agggcggcat gggcgaggtc tacaaaggcc
     7201 gcctcgtcgc ggagcacggc ttcgtgcggc cgatcgtcat caagctgatt cgcacggagc
     7261 tcctcgccga cgagcgcgcc gcgctcatgt tcatcgatga agcgcgcatg gccgcgggcc
     7321 tgcaccatcg gaacatcctg cagatcgtcg acttcgatcg cttccaaggt ggcgcgttca
     7381 tcgtcaccga atggatcgac ggttgcgatc tacgcacgct gctgaaggag atgcgcgcgg
     7441 cgccgcgcta cgacatcgcg ctcacgatca tcagcgagct cgcgaccggc ctcgatgccg
     7501 cgcacgatgc caccggcgca gacggcgcac cgctgcacct cgtccatcgc gacgtcagtc
     7561 cgagcaacgt actgctcggc aagcaaggcg agatcaagct cagtgacttc ggcatcgcga
     7621 aggcgcggat gcgcagctat cacaccgtgt ccggcacgat caaaggcaag gcgccgtaca
     7681 tggctcccga gcagatcctc ggcgaggtgc tcgatcggcg cgccgacatc ttcagcctcg
     7741 gtgtgttgct cttcgaggtg acgacccgta cgcgcctcta ttcgggaacc ggcgacgcga
     7801 atgcgatgaa gatgatcctc gcgggcgacg ttccggaccc tgccgagcgc cgccccggat
     7861 ttccgcccga gctcacgcag atcatccgca aggcactcgc gcgcgatcgc aatcgacgct
     7921 actcgacggc gggcgagctg gccgatgatc tcgacgaggt cgcacgttcg cgcaagtgga
     7981 gcttgtcgtg cacggcagtc ggcgaggtcg ctcgcgcgat cgcggcacgc gcaggccaat
     8041 cgacgccgac gccgggcgcc ggccgcttgt cgctcggagg tcagccgtga atccgggcgc
     8101 tgccgaatcg gcgcacgatc gcgtcggccc gtatcagctc gtcaaggcga ttggcgcggg
     8161 tggctcggca cgcgtcgatc tcgcgcgcat cgatcgcgcg tacggcttcc agcgccacgt
     8221 cgtcatcaag cgccccctcg agcatcttcg ctccgatcaa aaggccggcg attcgctgca
     8281 gcgcgaagcg cgcctgggcg gacgcctacg ccaccccaac ctcgtctccg tcctcgatgc
     8341 aggtcgccac gatggctacg actacctc
//
LOCUS       DATLBZ010000496         3860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8520553, whole genome shotgun sequence.
ACCESSION   DATLBZ010000496 DATLBZ010000000
VERSION     DATLBZ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8520553"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VMZ53_25050"
     CDS             complement(<1..192)
                     /locus_tag="VMZ53_25050"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS31803.1"
                     /translation="MDLFHDNPMYTAVRQMMGAFAKQSIRPISIKHDQEESMPWELMK
                     QAQAFGMTQTTLLDGRKKLT"
     gene            complement(192..1241)
                     /locus_tag="VMZ53_25055"
     CDS             complement(192..1241)
                     /locus_tag="VMZ53_25055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS31804.1"
                     /translation="MDFALDDDQQLIVETVRRFADRDLRTWAADSDRAGAPPDRLTHV
                     AGELGFFVDAVPAANDGMLEGAYSHATRALRGFELGRGCAALAALLETNVEPALAIEK
                     WGSAAAKKELFGSLASGGMAALAHDSRGNLEVVDGRVTGKIGPVPALAKASHVLVVAR
                     DLVFLAPTSALKITAITPSGWRAAHWASAQLDSYKPEHVLPAESAQHILAWARVSLAA
                     RAAGVATAAMEFADAYAKERVQFGQPIGKFESLIRLRDDAETGAVAAKQLALYAAWAL
                     DTNSQKAFDLASRARVLAGDVVAKATIDAVQIFGGYGFVNDYPVEKLMRDARAFEALH
                     GDERLGRVLAQKAVQ"
     gene            complement(1324..1926)
                     /locus_tag="VMZ53_25060"
     CDS             complement(1324..1926)
                     /locus_tag="VMZ53_25060"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS31805.1"
                     /translation="MGRPREHDETTRQELLAAAEKLLAERGLAALSVREAADAIGTTT
                     RAIYSVFGGRPGLLAALYVRGFEVLGGLIDALPQTRDPKADLVDAGVLGFRRFAVEHP
                     NLFRLTCERMAPDWEPGPTEHAAAMQTFDKLRALVRRCKDAGLIGGRSTAEIAFQFHA
                     FAQGLASVELQGWLPDLDGEAFCRSGLERFVTGLVAVKRK"
     gene            2002..3315
                     /locus_tag="VMZ53_25065"
     CDS             2002..3315
                     /locus_tag="VMZ53_25065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31806.1"
                     /translation="MEHAIVIGGSMAGLATAAALASRFERVTIVERKPVPDGVASVAP
                     HGRLPHVLLRGGFDALSRLLPGLLEEMHAAGATLSTAARGRWWSGGWRMRPDSDAIAP
                     LATRGLLETVVRRRVLALPNVAIQRGLVEGLVMADGAVRGLRVAGEELAADLVVDCGG
                     RGSGLPEWLVTAGFAAPTVSEAHVDVFYRALMFERRADDLDGDLFMVIQNIAPAKRLG
                     LALAVENNRWLLLCGAYFGERPPDDIAGITKFAESLPVRDLADLIRGRELVAEPTSYR
                     FTSSRRVSYPALPPGLAVIGDAHASFNPLYGQGMSVAALQAERLGQLIDRGIAQLADR
                     AHRELAAIADRAWQIAVGADFAYGETRGDKPRGVDRRNAYIRRVFAACTVDPVVSRAM
                     MEVQNMLAPPTSLLSPPMIARVLWGSRRARRRAVGASLQLSKNVA"
     gene            complement(3312..>3860)
                     /locus_tag="VMZ53_25070"
     CDS             complement(3312..>3860)
                     /locus_tag="VMZ53_25070"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31807.1"
                     /translation="LVPVGKDQVQHVEIARDIAQRINSAYGKDVLRLPKAKLEENAAI
                     VPGIDGRKMSKSYGNQIPLFAQPKQMKKVVNSIVTDSTPPETPKDPEASTIFQIYKAI
                     ASPEECNALAERYRAGIGWGDAKKALLDRLEVELAPARERYDALMADTAKIDALLAEG
                     AAKARATARKTLERVRAAVGIS"
BASE COUNT          635 a         1294 c         1235 g          696 t
ORIGIN      
        1 cgtcagcttc ttgcgtccat ccagcagcgt cgtctgcgtc atgccgaatg cttgggcctg
       61 cttcatcagc tcccacggca tcgactcttc ttgatcgtgc ttgatcgaga tcgggcgaat
      121 cgattgcttc gcgaatgcgc ccatcatctg acggacggcc gtgtacatcg ggttgtcgtg
      181 gaagaggtcc attactgcac cgccttctgc gcgaggacgc ggccgagccg ctcatcacca
      241 tgcagcgctt cgaacgctcg cgcgtcgcgc atcaatttct cgacgggata atcgttgacg
      301 aagccatagc cgccgaagat ctggacggca tcgatcgtcg ccttcgcgac cacatcgccg
      361 gccaacacgc gtgcgcggct cgcgaggtcg aacgctttct gagaattcgt atcgagtgcc
      421 cacgccgcgt acagcgcgag ctgcttcgct gccaccgcac cggtctcggc gtcgtcgcgc
      481 agccgaatca gcgactcgaa cttgccgatc ggctggccga actgcacgcg ctctttcgca
      541 tatgcgtcgg caaactccat cgccgcggtc gccacacctg ccgcacgcgc cgccaacgac
      601 acacgcgccc acgccaaaat gtgctgcgcg ctctcggcag gcagcacgtg ctcgggcttg
      661 tagctatcga gctgcgccga ggcccagtgc gcggcgcgcc aaccgctcgg cgtgatcgcc
      721 gtgatcttca acgccgatgt cggcgccaag aacacgaggt cgcgtgcaac gaccagcacg
      781 tgcgacgcct tcgcgagcgc aggcacgggc ccgatctttc ccgtcacgcg accgtcgacg
      841 acttctaggt tcccgcgcga atcatgcgcg agtgccgcca tgccgcccga cgcgagcgag
      901 ccaaacagct ccttcttcgc ggccgcgctg ccccacttct cgatcgcgag cgccggctcg
      961 acgttcgtct cgagcaacgc agcgagcgcc gcgcatccgc gcccgagctc gaagccgcgc
     1021 aacgcgcgcg tggcatgcga gtacgcgcct tcgagcatgc cgtcgttcgc cgcaggcacg
     1081 gcatcgacga agaagccgag ctcgccagca acgtgcgtca atcgatcggg tggagcgcca
     1141 gcgcgatcag agtccgcggc ccacgtccgc agatctcggt cggcgaatcg gcgcacagtt
     1201 tcgacgatca gctgctgatc gtcatcgagt gcgaaatcca tgacgtaagt ggtatcgcgc
     1261 tttcctaaaa cagtcactac ggactgtaaa ttgtaacggc ccggacagcc gctgcgattc
     1321 cagctacttg cgcttcaccg cgacaagtcc ggtcacgaaa cgctcgagcc cgctacgaca
     1381 gaacgcttcg ccgtcgaggt cgggcagcca gccctgcagc tcgaccgacg cgagtccttg
     1441 cgcgaacgcg tgaaactgaa acgcgatctc cgcggtgctc cggcctccga tcaagccggc
     1501 atccttgcag cgccgaacca aagcgcgaag cttgtcgaat gtctgcatcg cggcggcgtg
     1561 ctcggtggga ccgggctccc agtcaggcgc catgcgctcg cacgtgagcc gaaacaggtt
     1621 cggatgctcg acggcgaatc ggcgaaatcc gagcacgcct gcgtcgacga gatccgcttt
     1681 cggatcccgg gtctgcggca gggcatcgat caggccacca aggacctcga agccacgaac
     1741 gtagagcgcc gcgagtaacc ccgggcggcc gccaaacacg ctgtagatcg cgcgtgtcgt
     1801 ggtcccgatt gcgtcggcgg cttcccgcac cgacagcgca gcgagcccgc gctctgcaag
     1861 gagcttctcg gcagctgcca ggagctcctg gcgggtggtc tcgtcatgct ctctgggccg
     1921 gcccatatcg ctcgttgaca gcctactgca acgatgttac gaaacgatgt aacgtaacga
     1981 cgaaacgaaa tggagcggag aatggaacat gcgatcgtca tcggtggaag catggcgggg
     2041 ctcgccacgg cggcggccct cgcgtctcgt ttcgagcgcg tcacgatcgt cgagcgcaaa
     2101 cccgtacccg acggagtggc ttcggtcgcc ccgcacggtc gccttcccca tgtattgctg
     2161 cgcggcggct tcgacgcgct ctcgcggttg ctgccgggtc tgctcgaaga gatgcacgcg
     2221 gcgggcgcga cgctcagcac ggcagcgcgc ggacgctggt ggtccggcgg ctggcgcatg
     2281 cggccggact ccgacgcgat cgctccgctc gcgacgcgtg gtctgctcga gacggtcgtg
     2341 cgcaggcgcg tgctcgcgct gccaaacgtg gcgatccagc gcgggctcgt tgaagggtta
     2401 gtgatggcag acggcgccgt tcgcggattg cgcgttgcag gcgaggagct cgccgctgat
     2461 ctggtcgtcg attgcggtgg ccgtggttct ggactgcccg aatggcttgt cactgctggc
     2521 ttcgccgcgc cgaccgtgag cgaggcccac gtggacgtgt tctaccgcgc gctgatgttc
     2581 gagcgtcgcg ccgacgatct agacggcgac ttgttcatgg tgatccagaa cattgcgccg
     2641 gcgaagcgac tcggactcgc actcgcagtc gagaacaatc gctggttgct tctctgcggc
     2701 gcgtactttg gcgagcgccc acccgacgac atcgcgggca tcacgaagtt cgcggagtcc
     2761 cttccggttc gcgacctggc tgatctgatt cgcggccgcg agctcgtcgc cgaaccgacg
     2821 tcctaccgct tcacatcgag ccgccgcgtc agctatccgg cgctgccgcc gggcctcgct
     2881 gtcattggag atgcgcatgc gagcttcaat ccgctctacg ggcagggaat gtccgtcgcc
     2941 gcactccaag ccgagcggct cggtcagctc atcgatcgcg gcatcgcgca gctcgccgat
     3001 cgcgcacacc gcgagctcgc cgcgatcgca gaccgcgcat ggcagatagc ggtcggcgca
     3061 gacttcgcgt acggcgaaac gcgcggagac aagccgcgtg gcgtcgaccg ccgcaatgcg
     3121 tacatccgtc gggtattcgc tgcgtgcacg gtcgaccccg tcgtgtcgcg agccatgatg
     3181 gaagtgcaga acatgctcgc gccgcctaca tctctgttgt ccccgccgat gatcgcgcgc
     3241 gtactgtggg gaagtcgccg tgctcgcagg cgggccgttg gggcgagcct gcagctgagc
     3301 aaaaacgttg cttaggagat gccgacggcg gcgcggaccc gctcgagcgt cttgcgcgcg
     3361 gtcgcgcgag ccttcgcggc accttcggcg agcagcgcgt cgatcttcgc ggtgtcggcc
     3421 atcagcgcgt cgtagcgctc gcgcgccgga gcgagctcga cctcgagtcg atcgagcagc
     3481 gcttttttag cgtcacccca accgatgcct gcgcggtagc gttccgcgag cgcgttgcat
     3541 tcttcgggcg atgcgatcgc tttgtagatc tggaaaatcg tcgacgcctc gggatccttc
     3601 ggcgtctcgg gcggcgtcga gtcggtgacg atcgaattga cgaccttctt catctgcttc
     3661 ggttgcgcga acagaggaat ctggttgccg taactcttgc tcatcttgcg accatcgatg
     3721 ccgggcacga tcgccgcgtt ctcttcgagc tttgctttcg gcaggcggag gacgtctttg
     3781 ccgtacgccg agttgatgcg ttgggcgatg tcgcgcgcga tctcgacgtg ctgcacttgg
     3841 tctttgccga ccggcacgag
//
LOCUS       DATLBZ010000497        10542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2133536, whole genome shotgun sequence.
ACCESSION   DATLBZ010000497 DATLBZ010000000
VERSION     DATLBZ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133536"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1147
                     /locus_tag="VMZ53_25075"
     CDS             113..1147
                     /locus_tag="VMZ53_25075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ1255/VC2487 family glycosyltransferase"
                     /protein_id="HUS31808.1"
                     /translation="MRILYGVTGEGLGHAMRSRVMADHLRSRGHEVKLVASRRACSYL
                     RRYFDDVEEIPGFTLTYVKGGVGRTRTLYRNARVARANVRESIDLYRRPIVEFQPDVC
                     VSDFDSFAHMFGKLLGRPVISIDHQHVLDRCEHPALGRGRVPLTRALVRAKVPGCAHY
                     IVTSFYQPPLRPRAQGTTTLVGPILRPEVLALTPSTADHVLVYQTATRDLVAPLFALP
                     DYRFIVYGCHADRTLANVTFRTFDEPQFLADLASARAVICNGGYTLLSEALYLGKPVL
                     SVPLRRHGEQLLNALYLETLGLGRRADHPDANTFRDFLSRLPAPTTTPISAGNSHAFT
                     TIDQLLEELP"
     gene            1144..1497
                     /locus_tag="VMZ53_25080"
     CDS             1144..1497
                     /locus_tag="VMZ53_25080"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HUS31809.1"
                     /translation="MTPTSTPTTTSERLYASKSFGEFWERYQEIHAHPRTRAMHALAT
                     ASALGMVALAVARRNPKLALLAPVVDYGIAQLSHRSEGQRTQPLRKPLWHARAEWRLF
                     RKSLVDAEHYLHSRW"
     gene            complement(2564..3409)
                     /locus_tag="VMZ53_25085"
     CDS             complement(2564..3409)
                     /locus_tag="VMZ53_25085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31810.1"
                     /translation="MARYQDAIVTNVLGQQLQPGEQLHHWAYGVKQPNIGLIILLMCL
                     AILPGAIAVALMTKEYVIGLTSHRLIILQVSGGKAKVILKWDYYRNQMPAAQTSTGAL
                     FTHIKIKDAQRPFVAKFHRMGMPRNREHAMAIAAAISQPQLGAPPQGGPQGFLPPGQQ
                     QQGYPPPGAPQGYAPPQQGDAPPQQGYAPPQQGFAPPQQGGYPQQPQQGGYPQQPQQG
                     GYPQQPQQAQQGGYPQQPQQGYAQQGAQPQQPQQGGYPPGGQGGGGWPQGGGGQQGGG
                     GYGHG"
     gene            complement(3429..4352)
                     /locus_tag="VMZ53_25090"
     CDS             complement(3429..4352)
                     /locus_tag="VMZ53_25090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31811.1"
                     /translation="MKYVAFIYAVFAAGCILPVSTGAPMPATTVGKGSIGFAMSGEAP
                     TLDLIADKDNQQGSNADPIAYGAAPAAASTLTFSYGLTDDTDLEVAGEGALYYFILPA
                     PTGGSIGIRQHFNAGNDFDFGVAARFGHVGSTVESTDSNGTTTSSGARANYGALSAIL
                     QTKRGFVRPLAALQFMPAHIKRAPSDEPAFSFNGFASSITVALEFTGHNGVIAPYLTG
                     TNFYSDRFNNSGWFVSGGVMFALRKDRNRPHIDEVLPPTPPAGYPQYPPPYPQQPSAP
                     MGPPPPQPQGPPPPAQPPQGPPPPQPTTSPM"
     gene            4582..5355
                     /locus_tag="VMZ53_25095"
     CDS             4582..5355
                     /locus_tag="VMZ53_25095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31812.1"
                     /translation="MPTELFDELRIVPSLSILRTELFDELRIVPSLSIEPTLLFEPLR
                     IVTAPSLMSSRERRVKCPPAEGPKRHHREMRVSRWFVVMVLLLAASPALADRPVCIIG
                     RSDCRTIVLAEVSWGRVDSYQDMPDATRHHNHLLGDLELGMLVNVAARHGVGLSVGGA
                     SDPYRTDIHLIMRGRYRFWLDRRMGLEGSLAYGPANGVQGQVAAELLDQVGLFVSVGR
                     EHVPASSDATNVMFGVRFANLTALPALFIGYAAALATSD"
     gene            complement(5352..7421)
                     /gene="ligA"
                     /locus_tag="VMZ53_25100"
     CDS             complement(5352..7421)
                     /gene="ligA"
                     /locus_tag="VMZ53_25100"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HUS31813.1"
                     /translation="MLRAAMAQMDLFGSKPAAPPSGDSKDEYLKLVDELTEHDRRYYV
                     DAAPTISDVEYDKLLKKLRELEGQHPDWIVAWSPTKRVGHAPISDFPKVTRAVAMLSL
                     DNTYGHDDLREFHERVLKGLDNDSVTYSIEPKIDGFGIELTYKGGVLTLGATRGDGRI
                     GEDVTQNVRVVRGVALKLKEPVDIVVRGEIYMTKAEFAQINEERAKAGEDTFKNPRNT
                     AAGTIKQMDPREVAKRPLRTILYEVVDGERYSRGHLESLEYIKRLGLPTSPANSSASD
                     WDSLIAAVEQWQEKRDQLPYEVDGLVIKVDDFGQRAALGTTSKFPRWAIAYKFPARQV
                     TTRLLDLEANIGRTGTVTPVAILDPVDVSGTTVSRASVHNYDQVLRLGLGRGDRVLIE
                     KAGEIIPQILSVTEKGPGPAFEAPTECPSCGAPLEKEAGKVALLCMNRLGCPAQQLGA
                     IEFFASRGQMNIDGLGEKVVIQLVGAGLVKDVADLFVLDADQVAKLERFAEQSAKNLV
                     AAIAKAKETATFSRLLAALGIAHVGGVLAKPISQKYGTLSGMRAAAAAKDSAAFVADL
                     TEIEGVGETTAEGIDRFLRDPNVQVVLDKLIERGIDPKEPVVAVSDGPLTGKTFVVTG
                     TLSKPRADVQKAIEAAGGKVAGSVSKKTNYLVAGADTGKTKLEAAQKHGVTVIDEAEL
                     DKLLAKR"
     gene            7437..8408
                     /locus_tag="VMZ53_25105"
     CDS             7437..8408
                     /locus_tag="VMZ53_25105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31814.1"
                     /translation="MRGALLVPIAILAGCYSPHYQDCVISCASNACPSGFDCRDDKMC
                     RPSDTTGTCQNIESDARQLVDTSGDAPYPTGMWNAAQMVDVMMPDVSDPTLTDDLLEM
                     YFTHLGDIWVTKRSSTASAWSTPVAVPELGSPVSDHNPNMTPDGLTMLLASDRPGSTG
                     IDLWQSKRSSRSVPWGTPVRVPELESNVSDNSASLSYDELTIVFSSDRLGSNTDIFMA
                     TRTAGSPWTGVRELPELNIVNQEDTHPWLSPDKRTIYFMSDRTGGGDLYVARRATPAD
                     PFDMPQAISELNHGSNFDGDPWVSPDGHHIFFASDISGVSHIYEATR"
     gene            complement(8405..9532)
                     /locus_tag="VMZ53_25110"
     CDS             complement(8405..9532)
                     /locus_tag="VMZ53_25110"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS31815.1"
                     /translation="MTVQPKRTLKVEKLARIYDDEIAPVWGTRFGKMLLRNLAVPEKG
                     QVLDISCGTGYPTIEILRRMSDGSRLIAIDASSAMLDVARRKIADLGPLGKKGVFFRT
                     ESPVPKLSFADDVYDLVVCNLGLAEMPSVEVALRDFARVAKLGGEVRCTLPLAGTFQE
                     FHDLYREVLIKHDKHEALDRLDRHIARYPTIDHAERCMKSANLDGEIASEEFTLLFKS
                     SREFFFAPVIEYGPLAEWKEIAGSGQEMQDVFWYIKEAIDAYFDGRPFSVTVKAGCVI
                     GKKTELRAGDRSDAEISLDDDLEQFEDIDTGFPERPERMPAGSVRVSEDVSNVVSDED
                     VIDSHSPEEQDLDAFIDGKKRPSHLDRSEPIDLVDFEDDES"
     gene            complement(9600..>10542)
                     /gene="thiC"
                     /locus_tag="VMZ53_25115"
     CDS             complement(9600..>10542)
                     /gene="thiC"
                     /locus_tag="VMZ53_25115"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase"
                     /protein_id="HUS31816.1"
                     /translation="KLNIDMFIETLIEQAEQGVDYFTIHAGVRLAYIPLTANRVTGIV
                     SRGGSIMAKWCLAHHKENFLYTEFERICEVMKKYDVAFSLGDGLRPGSTADANDRAQL
                     AELETLGELTDIAWKHDVQTMIEGPGHVPMHKIKENVDLQMKICKEAPFYTLGPLVTD
                     IAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKDGVIAYKIAAHAADL
                     AKGHPGAHKRDDALSKARFEFRWLDQFNLSLDPDTAKDFHDETLPADGAKVAHFCSMC
                     GPKFCSMKISEEVREHLAQKSEEFTSSGGELYVTKVP"
BASE COUNT         1726 a         3407 c         3413 g         1996 t
ORIGIN      
        1 gcgctctaca agcgactcga taccgaggcc aaccagcgcg agacaacgaa gcggcagatg
       61 gtgatcgccg cgctcgagcg gtacctcgat caaactcact aggaggctct cgatgagaat
      121 tctctacggc gttaccggcg aaggcctcgg tcacgcaatg cgcagccgcg tgatggccga
      181 ccacctccgc tcgcgcggcc acgaggtcaa gctcgtcgcg tcgcgccgcg cgtgcagcta
      241 cctgcgccgc tatttcgacg acgtcgagga gattcctggc ttcacgctga cgtacgtcaa
      301 aggtggcgtc ggccgcacgc gaaccctcta tcgcaatgcg cgagtcgcac gcgcaaacgt
      361 tcgcgagagc atcgacctct accgccgccc gatcgtcgag ttccaacccg acgtctgtgt
      421 gagcgacttc gactcgtttg ctcacatgtt cggcaagctg ctcggccgcc ccgtcatctc
      481 gatcgatcat cagcacgtcc tcgaccgctg cgagcaccca gcgctcggcc gtgggcgtgt
      541 gccgctcacg cgcgcgctcg ttcgcgcgaa ggtgccgggc tgcgctcact acatcgtgac
      601 gtcgttctat caaccgccgc ttcgcccacg cgcgcaaggc acgacgacgc tcgttggtcc
      661 gatcctgcgt cccgaagtcc tcgcgctcac gccgagcaca gccgaccacg tgctcgtcta
      721 tcaaacggcc acacgcgatc tcgtcgcgcc gctcttcgcc ctccccgact accgcttcat
      781 cgtctacggc tgccacgccg accgcaccct cgcgaacgtc acattccgca cgttcgacga
      841 accgcagttc ctcgccgacc tcgcctcggc gcgcgccgtc atctgcaacg gcggttacac
      901 gctgctatcc gaagcgctct acctcggcaa gccagtcctc agcgtgccgc tgcgtcgaca
      961 cggcgagcag ctgctcaacg cgctctacct cgagacgctt ggcctcggcc gccgcgccga
     1021 ccatcccgac gcgaacacgt ttcgcgactt cctctcgcgc ctgccggcac ccaccacgac
     1081 gccgatctcc gcaggcaact cccacgcctt cacgacgatc gatcaactgc tcgaggagct
     1141 gccatgactc caacgtcaac gcccaccacg acgagcgaac ggctgtacgc atcgaagagc
     1201 ttcggcgaat tctgggagcg ctaccaagag atccatgcgc acccgcggac acgcgcgatg
     1261 cacgcgctcg caactgcctc cgcgctcggc atggtcgcgc tcgcggtcgc gcgccggaat
     1321 cccaagctcg cgctgctcgc gccggtcgtc gattacggaa tcgcgcagct ctcgcatcgc
     1381 agcgagggcc agcgaacgca gcctctgcgc aagccgctgt ggcacgcgcg cgccgagtgg
     1441 cgcctgttcc gcaagtcgct cgtcgatgcg gagcactacc tgcactcgcg ctggtgagct
     1501 gcgccagccc gcgctcgtcc gacatcgacg aagaaagcgt atcggtgcgt ggcagcgtcg
     1561 acggcggcgg tcgagatcgg cgcgcgacgc gaacagccga gcgtgcccac gtgggcacgg
     1621 cagcacggat gtgtctcgtg cccgcgtggg cacggcagtt cgcatgtgtc tcgtgcccgc
     1681 gtgggcacgg cagctcggat gtgtctcgtg cccgcgtggg cacagcacaa cgcatcgaag
     1741 tcttcccgag gcaagctatg agcggatcgt gacgatgaac cctttgtgct tggagtcagc
     1801 atcgtttcgt ctcgcttcat tcgtgtgcga gcgcctggcc cggcggggga gcggcgcgcg
     1861 gggcccaatc acaagggccc ctgcgcgcgc cgcgatccca ccgccggccc aggcgcggct
     1921 cgcagcatgt cgttcgatat ttcttctctg acgatgttcg agcacgtcgc cccactggcg
     1981 aggcgcggct cgcagcatgt cgtgactccg atgcgatcgg cgagccgagg cgccgccagc
     2041 gcgtgacgaa agcgattccc gccgcgacga agcggcgcgt gctcgaacgc gacgagggtc
     2101 gctgtcagat gcccggttgt ccctcgagga agcatctgca ctttcaccac atcgtgtgtc
     2161 agtcgcacgg cggcggacac gaggacgcga acttgctcct gctttgcggc ggccaccatc
     2221 ggttggctgc acgatggcct gttgagtatc accggacgtg caccgaacga gctcgcattc
     2281 acgcgcaacg gatgcgccct gcgcagcgca aacgatgacg cgcgacctcg agtgagtggg
     2341 cgcgatagcc acgcggcgcg caagcacgcg ggcgtgcctt cgagtgaaga tcgcgtgcgc
     2401 gcaggcaacg acaacgcggg acttgcagaa caggcattgc ggacgctcgg cttcaaggcg
     2461 cacatcgcgt tgcgcgcggt cgagcaggcg tgtgcccacg tgggcatgga ttcgcgcgat
     2521 cttgtgtggg tctggcgaac gcgcgcgcac ttttgcgtgc gcgctaaccg tggccgtagc
     2581 cgccgccacc ttgctggccg ccaccacctt ggggccagcc acctccgcct tgtccgcccg
     2641 gcggataacc gccttgttgc ggttgctgcg gttgggcgcc ttgttgtgcg tagccctgct
     2701 gcggctgctg cggatagcca ccttgttgcg cctgctgcgg ctgctgcgga tagccacctt
     2761 gttgcggctg ctgcggatag ccgccttgtt gcggctgctg cggataacca ccttgttgcg
     2821 gcggcgcgaa gccttgctgc ggcggcgcgt agccttgctg cggcggcgcg tcgccttgct
     2881 gcggcggcgc gtaaccctgc ggcgcgcccg gcggcgggta gccttgttgt tgctggccgg
     2941 gtggcaggaa gccttgcggt ccgccttgcg gcggcgctcc gagctgcggc tgcgagatcg
     3001 cagcggcgat tgccatcgcg tgctcgcgat tgcgcggcat gcccatgcga tggaacttcg
     3061 cgacgaacgg acgctgggcg tccttgatct tgatgtgcgt aaacagcgcg ccggtcgatg
     3121 tctgcgcggc cggcatctgg ttgcggtagt agtcccactt gaggatgacc ttggccttgc
     3181 cgccgctgac ttggagaatg atcaagcggt gggaggtgag gccgatgacg tactccttcg
     3241 tcatcagcgc gaccgcgatc gcgccgggaa ggatcgcgag gcacatcagc aggatgatca
     3301 ggccgatgtt tggctgcttg acgccgtacg cccagtgatg aagctgctcg cccggctgca
     3361 gctgttggcc gagcacgttc gtgacgatcg cgtcttgata acgagccata gcgctcgttg
     3421 tatcaccgtc acatcggcga tgtcgtcggc tgcggcggcg gcggtccctg cggtggttgc
     3481 gcgggtggcg gcgggccttg cggctgcggc ggcggtgggc ccatcggtgc cgacggttgc
     3541 tgcggatacg gcggcggata ctgcggatat ccggcgggcg gagtgggcgg caacacctca
     3601 tcgatgtgcg ggcggttgcg gtctttgcgc agagcgaaca tcacgccacc ggagacgaac
     3661 cagccgctgt tgttgaagcg gtcgctgtag aagttcgtgc ccgtgaggta cggcgcgatc
     3721 acgccgttgt ggcccgtgaa ttcgagggcg acggtgatcg aggacgcgaa gccgttgaac
     3781 gagaaggcag gttcgtcgga cggcgcgcgc ttgatgtgag ccggcatgaa ctggagtgct
     3841 gcgagtgggc gcacgaagcc gcgcttcgtc tgcaggatcg cggacagcgc gccgtagttc
     3901 gcgcgtgcgc cactcgacgt cgtcgtgccg ttcgaatcgg tggactcgac ggtcgagccg
     3961 acgtgaccga aacgcgcggc gacgccgaag tcgaagtcgt tgcccgcgtt gaagtgctgg
     4021 cggatgccga tcgagccgcc cgttggcgcg gggaggatga agtaatagag cgcgccttcc
     4081 cccgcgactt cgagatcggt gtcgtcggtg aggccgtaac tgaatgtgag cgtcgatgcg
     4141 gctgcgggcg cggcgccgta ggcgatcgga tcggcgttgc tgccttgttg gttgtctttg
     4201 tcggcgatga ggtcgagcgt cggcgcttcg ccgctcatcg cgaagccgat gctgcctttg
     4261 ccgaccgttg ttgctggcat gggcgcgccc gtcgagacag gcaggatgca gccggcggcg
     4321 aatactgcat aaatgaaggc gacgtacttc atgaattccc ccggagctat tcgatcacga
     4381 ctcgggggca gcgcgccaga tggcgaagcc tcactgcgct ggcaaagtgc cgcgcgcggc
     4441 tggcgtcaca atttcaggac gctgacggat ccgccgaaga acagcaccgc agcgactaga
     4501 tggcgcaggt ccgacgagta accatccact tttccgacgg agctgttaga cgagttacga
     4561 atcgttccgt cgctatcgat cttgcccacc gagctgttcg acgagttgcg gatcgttccg
     4621 tccttgtcga tcttgcggac ggagctgttc gacgaattgc ggattgtccc gtcactatcg
     4681 atcgagccga cgctgctgtt cgagccgttg cggatcgtca ccgcgccgag cttgatgtcg
     4741 tcacgtgaac gtcgcgtgaa gtgcccaccg gctgaaggcc caaagcgtca tcatcgcgaa
     4801 atgagagtct cgcgttggtt cgtcgtgatg gtgcttttgc tggcggcgag cccggctttg
     4861 gcggataggc cggtctgcat cattggccgc agtgattgca ggaccatcgt gcttgccgag
     4921 gtctcatggg gccgcgtcga ctcctatcag gacatgccgg acgcgacgcg gcatcataac
     4981 cacctcctcg gcgatctgga gctcgggatg cttgtgaacg tggccgcgcg acacggagta
     5041 ggactgtcgg ttggcggagc ctcggatccg taccgcaccg acatccatct gatcatgcgt
     5101 ggccgttacc gcttctggct cgacagacgc atgggactcg aaggctcgct cgcctatggc
     5161 ccggccaacg gtgttcaagg gcaggtagcc gcagagctcc tcgatcaggt cgggttgttc
     5221 gtgtccgttg gcagggagca cgtaccggct tcgtcggatg ctacgaacgt gatgttcgga
     5281 gtgcgcttcg cgaacctgac agcgctgccg gcactgttca tcggctacgc cgccgctctg
     5341 gcgacgagcg actagcgctt cgccaggagc ttatcgagct cggcctcgtc gatgaccgtc
     5401 acgccgtgct tctgcgcggc ctcgagcttc gtcttgccag tgtccgcgcc ggcgacgaga
     5461 tagttcgtct tcttgctgac cgagcctgcg actttgccgc cggcggcttc gatcgctttc
     5521 tgcacgtcgg cgcgcggctt cgagagcgtg cccgtgacga cgaaggtctt gccggtcagc
     5581 gggccatcgg agactgcgac gacgggctcc ttcggatcga tgcctcgctc gatcagcttg
     5641 tcgagcacga cctgcacgtt tggatcgcga aggaatcggt cgatgccttc tgcggtcgtc
     5701 tcgcccacac cttcgatctc ggtgaggtcg gcgacaaagg ccgcgctgtc ctttgcggcg
     5761 gccgccgcgc gcatgccgga gagcgtgccg tacttctgcg agatcggttt cgcgagcacg
     5821 ccacctacgt gcgcgatgcc gagcgccgcg agtaggcgcg agaacgtagc ggtctctttc
     5881 gccttcgcga tcgcggcaac gaggttcttc gccgactgct ccgcgaaccg ctcgagcttc
     5941 gcgacttggt cggcgtcgag cacgaacaga tcagcgacgt ctttgacgag ccctgcgccg
     6001 acgagttgga tcacgacctt ctcgccgaga ccatcgatgt tcatctggcc gcgggacgcg
     6061 aagaattcga tcgcgccgag ctgttgagcc ggacagccga gccggttcat gcacagcagc
     6121 gcgaccttgc ccgcttcttt ctcgagcggc gcgccacacg agggacactc ggtcggtgcc
     6181 tcgaacgcag ggcccggtcc tttctcggtg acgctcagga tctgcgggat gatctcgccg
     6241 gccttctcga tgaggacgcg gtcaccgcga ccgaggccga ggcgcaacac ctggtcgtag
     6301 ttgtgcacgc tcgcgcgcga gacggtcgtg ccggagacgt cgacgggatc gagaatcgcg
     6361 accggcgtga cggtgcccgt gcgaccgatg ttcgcctcga gatcgaggag ccgcgtcgtc
     6421 acctggcgcg cggggaactt atacgcgatc gcccagcgcg gaaacttcga cgtcgtaccg
     6481 agcgcagcgc gctgaccgaa atcgtcgacc ttgatcacga ggccatcgac ttcgtacggc
     6541 agctggtcgc gtttctcctg ccactgctcg accgcggcga tcagcgagtc ccaatcgctc
     6601 gccgaggagt tcgctggcga cgtcggcaag ccaagccgct tgatgtactc gagcgactcg
     6661 aggtgaccgc gcgagtaacg ctcgccgtcg acgacttcgt acaggatcgt gcgcagtggg
     6721 cgcttggcga cctcgcgcgg atccatctgc ttgatcgtgc ccgcggcggt gttgcgcgga
     6781 ttcttgaacg tgtcctcgcc ggccttcgcg cgctcttcat tgatctgcgc gaactccgcc
     6841 ttcgtcatgt agatctcgcc gcgcacgacg atgtcgacgg gctccttgag cttgagcgcg
     6901 acacctcgca cgacgcgtac gttctgcgtg acgtcctcgc cgatgcgccc atcaccgcgc
     6961 gtcgcgccga gcgtcagcac gccgcctttg taggtgagct cgatgccaaa gccgtcgatc
     7021 ttcggctcga tcgagtacgt gacgctgtcg ttatccagcc ccttgagcac gcgctcgtgg
     7081 aattcgcgta ggtcgtcgtg gccgtacgtg ttgtcgagcg agagcatcgc gacggcgcgc
     7141 gtgaccttcg gaaagtcgct gatcggcgca tggcctacgc gcttcgtcgg tgaccacgca
     7201 acgatccagt cggggtgctg gccttcgagc tcgcgcagct tcttcagcag cttgtcgtac
     7261 tcgacgtcgc tgatcgtcgg cgctgcatcg acgtaatagc ggcgatcgtg ctcggtcagc
     7321 tcgtcgacga gctttaggta ctcgtctttc gaatcgccgc tcggcggggc agccggcttg
     7381 ctcccgaaca ggtccatctg cgccatagcg gcacgcagca tagtacgatg tagcgaatgc
     7441 gcggcgcgct actcgtcccg atcgcgatcc tcgcgggttg ttactcaccg cactaccagg
     7501 actgcgtcat ctcgtgtgcc agcaacgctt gtccatcggg cttcgattgc cgtgacgaca
     7561 agatgtgcag accctctgac acgactggca cctgccagaa catcgaaagc gacgcgcgac
     7621 aactcgtcga tacgagcggc gatgcgccct acccgaccgg catgtggaac gctgcgcaga
     7681 tggtcgacgt catgatgccc gacgtctccg atccgaccct gaccgacgat ttgctcgaga
     7741 tgtatttcac gcacctgggt gacatctggg tgacgaaacg gtccagcaca gccagcgcct
     7801 ggtccacacc ggtcgccgtg ccggagctcg ggtcgccggt gagtgaccac aacccgaaca
     7861 tgacgcccga tggcttgacg atgttgctcg catcagaccg cccgggatcc acggggatcg
     7921 atctgtggca atcgaagcga tcgagtcgct cggtgccgtg gggcacgcct gtgagggtcc
     7981 cggagctcga gagcaacgtg tccgataact ccgcgagcct ttcatacgat gagctaacga
     8041 tcgtgttctc gtcggaccgc ttgggcagca acaccgacat cttcatggcg acacgcacag
     8101 ccggatcgcc gtggaccggg gtcagggagc ttcccgagct caacatcgtc aaccaagagg
     8161 acacccatcc gtggcttagc ccggataagc ggaccattta cttcatgagc gaccgcacgg
     8221 gtggcggtga tctctacgtc gcaagacgcg caactccggc cgacccgttc gacatgccgc
     8281 aagcaatctc agagctaaac cacggctcga actttgacgg tgatccgtgg gtttctccgg
     8341 acggtcacca catcttcttt gcgagtgaca tcagcggcgt gtcccatatc tacgaagcga
     8401 cgcgttagct ctcgtcgtct tcgaagtcta caaggtcgat cggctcgctg cgatcgaggt
     8461 gcgacggacg cttcttgccg tcgatgaatg catcgaggtc ctgctcttcc ggcgagtgcg
     8521 aatcgatcac gtcctcgtcg gagacgacgt tcgacacatc ctcgcttacg cgcaccgagc
     8581 ctgccggcat ccgctcggga cgctccggga agccggtgtc gatgtcctcg aactgctcga
     8641 gatcgtcgtc gagcgaaatc tctgcgtcgc tgcgatcgcc ggcgcgaagc tcggtcttct
     8701 tgccgatcac gcagcccgct ttgaccgtga cggagaacgg ccggccatca aagtacgcgt
     8761 cgatcgcttc cttgatgtac cagaacacgt cctgcatctc ttggccggag cccgcgatct
     8821 ctttccactc ggcgagcggg ccgtactcga tgacgggcgc gaagaagaac tcgcgcgagc
     8881 tcttgaacag cagcgtgaac tcctcggacg cgatttcacc gtcgaggttc gcgctcttca
     8941 tgcagcgctc ggcgtgatcg atcgtcgggt agcgcgcgat gtggcggtcg agacgatcga
     9001 gcgcttcgtg cttgtcgtgc ttgatgagga cctcgcgata gaggtcgtgg aattcttgga
     9061 acgtgccggc gagcggcagc gtgcagcgaa cctcaccgcc gagctttgcg acgcgcgcga
     9121 aatcgcggag ggctacttcg acagacggca tctcggcgag gccgaggttg cagaccacga
     9181 ggtcgtagac gtcgtcggcg aacgagagct tcggcaccgg gctctcggtg cggaagaaca
     9241 cgcccttctt gccgagcggt ccgaggtccg cgatcttgcg acgagcgaca tcgagcatcg
     9301 cgctcgacgc gtcgatggcg atcaggcgcg agccatcgct catgcggcgc aggatctcga
     9361 tcgtcgggta gccagtgccg cacgaaatgt cgagcacctg ccccttctcg ggcactgcga
     9421 gatttcgcag cagcatcttg ccgaaacgcg tgccccatac gggcgcaatc tcgtcgtcgt
     9481 agatgcgcgc cagcttctcg accttgagcg tgcgcttggg ctgtaccgtc actgcctaga
     9541 tacccggaat catgacggaa cggtgacggc ttgtctacaa ctgcgtactg ggcccccgct
     9601 tacggaactt tcgtcacgta cagctcgcca ccagaggacg tgaattcctc cgacttctgg
     9661 gcgagatgct cgcgcacttc ctcggaaatc ttcatcgaac agaacttggg cccgcacatc
     9721 gagcagaagt gcgcgacctt ggctccgtcg gcgggaagcg tctcgtcgtg gaagtccttc
     9781 gcggtgtccg ggtcgagcga caggttgaac tggtcgagcc agcggaactc gaagcgcgcc
     9841 ttggacaggg cgtcgtcgcg cttgtgagcg cccgggtggc ccttcgcgag atcggccgca
     9901 tgcgccgcga tcttgtaggc gatgacgccg tccttcacgt cgtcgcggtc cgggagaccg
     9961 aggtgctctt tcggcgtgac gtagcagagc atcgccgtgc cgaaccagcc gatcatcgcc
    10021 gcaccgatcg cgctcgtgat gtggtcgtag ccaggcgcga tgtcggtgac gagaggcccg
    10081 agcgtataga acggcgcctc tttgcagatc ttcatctgca ggtcgacgtt ctctttgatc
    10141 ttgtgcatcg gcacgtggcc ggggccttcg atcatcgtct gcacgtcgtg cttccacgcg
    10201 atgtcggtga gctcaccgag ggtctcgagc tctgcgagct gcgcgcgatc gtttgcgtct
    10261 gccgtcgagc cgggacgcag gccatcgccg agcgagaacg cgacgtcgta cttcttcatg
    10321 acctcgcaga tgcgctcgaa ctcggtgtag aggaagttct ccttgtggtg cgcgaggcac
    10381 cacttcgcca tgatcgagcc gccgcgcgag acgatgcccg tcacgcgatt cgcggtgagc
    10441 gggatgtacg cgagacgcac gcccgcgtgg atcgtgaagt agtcgacgcc ctgctcggcc
    10501 tgctcgatca acgtctcgat gaacatgtcg atgttcagct tc
//
LOCUS       DATLBZ010000498        14892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2881475, whole genome shotgun sequence.
ACCESSION   DATLBZ010000498 DATLBZ010000000
VERSION     DATLBZ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14892
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2881475"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VMZ53_25120"
     CDS             <1..591
                     /locus_tag="VMZ53_25120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31817.1"
                     /translation="KALFELYDAFGSASLDTTLWTNVRGSTLNNGRLVVGGGGQTDNG
                     VISKMSFGVNNAVDFIIVASQATTAMPGFWAGFEGSTTDGPPYTIWWTLDTSNMIAPS
                     YKITTASTDYRGTPFAFTTAAHYYSVEHYGKGTMYRYDDVPYDTQAYDVAYTSPLFIR
                     LWNYNSSPSVAYDMVRVRQAVDPPPAVVVGAPVAKP"
     gene            complement(592..1248)
                     /locus_tag="VMZ53_25125"
     CDS             complement(592..1248)
                     /locus_tag="VMZ53_25125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31818.1"
                     /translation="MSGVRFDDLFAAFVRIPGAVGRYFADRKHRKWMRKAPTFRVRDM
                     PENMFAKIVGHVRPFRDRAIEAPLSKRLCVYYDVSIDAMVGNSMLRTLATVQDGIPFV
                     LRDDSGAAVIDPAHAFVSSGIDAMSESRPLEADAAQRELLVRTGLAGRTPAMLDSLRF
                     REAVLELDEMVAVFGGGIREPDPEGVATMTYRDGAPTRLHLTGTAKFPLFISDDPRSF
                     "
     gene            complement(1245..2084)
                     /locus_tag="VMZ53_25130"
     CDS             complement(1245..2084)
                     /locus_tag="VMZ53_25130"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HUS31819.1"
                     /translation="MRGLALVMLLAACGSSDDAPPTKTYGGDRPVDLRTPATLTEGKK
                     YPLVVILHGFGANGFAQYAFFNASALVNSDRALVLAPDGTVNSSGQQFWNADPACCDF
                     EGKNPDDVAYIGGLIDDVVADWPIDKNQIYLIGHSNGGYMSYRMACERADVIAAVASL
                     AGNAATNASACTPDKPVNVLHMHGTADATVPFASGGTGAVASVSQWATHDGCGTTRTS
                     TVTLDLDSQVAGAETHGETTSGCPSGVAVDLWTMEGSSHIPLFNANAIDDIFSWLTAH
                     KRG"
     gene            2120..3031
                     /gene="miaA"
                     /locus_tag="VMZ53_25135"
     CDS             2120..3031
                     /gene="miaA"
                     /locus_tag="VMZ53_25135"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HUS31820.1"
                     /translation="MPPRLVVIVGPTGAGKTRLSLELAMRVGGEVISCDSQQVYLGMD
                     IGTGKVTADERSRVPHHLLDVVKPDQEMTMARFIDMADAAVAEVASRGKPVIVCGGTG
                     LYVRGLLLGLFEGPPADTELRKEIQAMGIAAIRDELERVDPKAASKIDRNDEKRMVRA
                     LEVYRLTGEPMSAHQARHDHKTMARRYDAKLVGLAPEREQLYRQIDARVDEMMAAGFE
                     QEVAALRAAGYAPPLRSQQAIGYDELHQVAAGALDRGRAIELIKRNSRHYARRQLSWY
                     RPDQTITWYASPAAVDLAELERYLAGL"
     gene            3032..4024
                     /locus_tag="VMZ53_25140"
     CDS             3032..4024
                     /locus_tag="VMZ53_25140"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HUS31821.1"
                     /translation="MSTTEAPPQLTDRMILEFERPIIDLEKKISELRGLSTDTVDFSA
                     EIRKLEQKARRLQKEVFADLSAQQKVQLARHPARPYMNDYIGLLMDDFVELHGDRSFR
                     DDPAIVGGLAMFDEWEVLVLGHQKGRNTKDNMHRNFGMARPEGYRKATRLMRMASRFR
                     RPIICFIDTPGAYPGLGAEERGQAEAIAKALQVMASLECPLLSIVIGEGGSGGALALG
                     VTDRILMLEYSIYSVISPEGCASILWRDPAKIAEAATQLKLTAPDLLHLGICDEIIPE
                     AAGGAHRSPAQTAAKMRMAIKKHLKELVELPPAELVERRYQKFRKMGAFIESPS"
     gene            4031..6088
                     /locus_tag="VMZ53_25145"
     CDS             4031..6088
                     /locus_tag="VMZ53_25145"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUS31822.1"
                     /translation="MYYELALISVAVAGGYWGWYFIRHQTTRLYGMLQLGAALLSLVG
                     LLGRKIGESSLGIAGAVGVGAGVCLLVLGPLVRGFARRLAGAERFDAAKRMFDVAEIL
                     APGSGVDDEKLLLAAMREIRDGNIETTVEALTAAKQRAPADARLAIDERIAMLYLAAY
                     RWDEAITHAEAHLFGAIPAMGEASSSHAALRRALGIAPAVWVELLGAYGYVGDLDRAA
                     SMLARLEEVCAGRPDASVWLHRGRLIALALAGRVSAVQALVEPRRSRHMKPAARTYWL
                     GVAYERHGEIAAAEAAYAKARSRSRGRPRILIDQALARLPVAKPVELSTEASAIIERV
                     EATPPPAVTERMHRQGPTATRILAAAMIVTAATIALTLGNSADLGVLIRDGALVHTLV
                     KAGQWWRLFTSIFVHAGGLHFLVNLVGLWMLGRLAEELFGSWRMAAIFALAGLGGAVA
                     SLLATNANISTGASGAILGLLGAVFAELTVHRKKHRLAWTRGVWGSLAVVALAQVGTD
                     FMYRLTDHWAHAGGLVVGALAGLAFSPNLEWKNARVHAARVITAIFAAAVITSFAFTV
                     RTSIVDSLTASPEFAVELVSPYDKMPATGNIDAYISGTANRAKENFSIERVESAPDTL
                     VTLPAGWTGQEMIGWLPSELGDDQKVRIIVARAPDRVLTLYLPESVVRGAPEYFTWLI
                     PRL"
     gene            6099..6716
                     /locus_tag="VMZ53_25150"
     CDS             6099..6716
                     /locus_tag="VMZ53_25150"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HUS31823.1"
                     /translation="MRIALVLVLVATASAAADPLGDCYDELDARHITYKKTKKSGIKK
                     AVEITGTLGGVEVSGAEPLVIDCSLAVSLDEAGRYMKALGMESAHFASAYSRRMVRGT
                     NHPSKHSYGLAIDVSSFTGPDLGTVRVDKDYEQGLGDAVDCVGQPLTQGGAILKVLQC
                     QLVRSGLFHLVLSPDYDDAHHDHFHLEAIPWPDRSSLRSSSPAIH"
     gene            complement(6739..7428)
                     /locus_tag="VMZ53_25155"
                     /pseudo
     CDS             complement(6739..7428)
                     /locus_tag="VMZ53_25155"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
     gene            complement(7431..7934)
                     /locus_tag="VMZ53_25160"
     CDS             complement(7431..7934)
                     /locus_tag="VMZ53_25160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31824.1"
                     /translation="MHILRLTTVFAGVAALFTAAGIGRVAAYASVDKIGGRRHAMYMA
                     ARAHAAASAGLMLIAVIPHGELPERGPSWLLIPIMGAIAGALCGALIGVVCSGGSTVR
                     ISDVMALAIKKPSEALRQLLDPEDLVKLGAAVRHRTTQMFEGIFEPAEKRPTEKPDAK
                     RVEPPRE"
     gene            8220..9185
                     /locus_tag="VMZ53_25165"
     CDS             8220..9185
                     /locus_tag="VMZ53_25165"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HUS31825.1"
                     /translation="MANANPSNRAYAAAHFALELDGKEDVGMFRSVEGGGVKADVMTY
                     QPAPSQENGGYQKWRQLGKPKFDDIKLQVGMSMAQPFYAWLADFFVGKATRKTGAIVA
                     ADFYYNERARRDFKGAMIRELTFPKLDAADKNAIYMNISVCVEDIEFKKGSGTKLKVP
                     EKTSGQKLWTACNFTFKLDGLDEACRRVTKIDSFTVKQAVAEYHAGGFKAAIKTPTAM
                     EFPNLIFYVPEADAQPFMEHMQASVGFGKDLGKGKVRDPKTLNGSISTFDNENRHLLD
                     VEFKGADIVSVAPEKSESTTEEVKLVKIELYIESMELKYPSAELE"
     gene            complement(9186..9335)
                     /locus_tag="VMZ53_25170"
     CDS             complement(9186..9335)
                     /locus_tag="VMZ53_25170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31826.1"
                     /translation="MLAFVLTPVEAFAQVDDPDELEAVTDLLGAAKEEAVKRWFSLRD
                     EAIRN"
     gene            9451..10581
                     /locus_tag="VMZ53_25175"
     CDS             9451..10581
                     /locus_tag="VMZ53_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HUS31827.1"
                     /translation="MPRSKVAVLRVRPDHVLEDIDRLYELADVKAALKPGAQTILKDN
                     ISWHFPFPGANTTPWQLEGTVRALHSRGYNDLVCVQNKTVVTDAFKGEDLNGYLPIFK
                     RYDIPVRYNFKPEDMTWSVYRPKAKMNVLDTIFPEGIQIPDAFHGTNIIHLPTVKCHI
                     YTTTTGAMKNAFGGLLNTKRHYTHSWIHETLVDLLAIQKEIHSGLFAVMDGTTAGDGP
                     GPRTMRPVIKDVMLASDDQVAIDAVAASMMGFDPMTLKYISMADEQGLGNGRRENIDV
                     VGDEELANQRWGFSVGDNGASMVGDIMWFGPMKRFQKLFFHTPLVNLFVMGSEAYHDY
                     YRWPLRDRRIFEQWRDSTTWGRLFDHYQVHGTLAPQDVAKVG"
     gene            10602..11156
                     /locus_tag="VMZ53_25180"
     CDS             10602..11156
                     /locus_tag="VMZ53_25180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31828.1"
                     /translation="MRVVLLTLLVGCGFEHGAWGPASTVDAGDDVDAAIDAEAMVIVD
                     APPDPPPSTKSWVVIETLTVPVNGQSVTSQTALANGVTYHLRASGTWVIQSTPGTQAD
                     AEFWDFTNTAGPQEGVTGVDCGLAVDDTTVDTNKTPKWGAYSASHTYEIDWVGDGTTI
                     VALMHDGNYSNNTGSLSLAILAYQ"
     gene            11207..13171
                     /locus_tag="VMZ53_25185"
     CDS             11207..13171
                     /locus_tag="VMZ53_25185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31829.1"
                     /translation="MRWLPVLVVAGCAEPSAPAVPQVSVAPDVEQPTPRSPAWDPWAY
                     PQVVPPKPPEPWALSTSAASIAEPAKRLVTSIHGASDIAVLAVTEDGRAAVTADIRSV
                     RFWPRLDGKHEPVVVAMRPPAALAIARDGDGLLVAGLDAIGQLELVRLTADGESLLRV
                     AAASARPIREIHATARGVIAVRDDDAIALYDAAGELRGELAPVARQRVRAVAVRHDAA
                     IALVESGGTMHGQWLDVDATGVTRGHSTPALPFRDPAFALSTDHQRLVGTDQRGNVVV
                     VDANTGRALQHRWKNKDDAALHVVGFIDRSALAIDVGAPFVALHAKRDFEQSEEIAGQ
                     HFVTVDGAVVGVEGAALVIGRHGKPVQYLGYRMASPITAVPRDDGFLITDGTALAELG
                     PDLRARKLFKIAPKDGELFNTQLVDADHVVTAFAPGRDNPVGYVFADVSKGTVTPLGD
                     GYASFSRENGLLAIQQLSRIDVRRYDRKQGKLGPASTLHVSSWVPFVWLDSKRGDFAL
                     VTLDFDARDAELDVFRLRDDVERLKTRNVKPAERWWDAQNMDTLLGETWRVSRRRSSP
                     DGSLVAEVSTDGRMTLHDATGAERWMVASGGAMDVLWTPAGELVAFGAGIARVDIETG
                     ALRERQCGWQFGLWPASTTPISASALCEAP"
     gene            13168..>14892
                     /locus_tag="VMZ53_25190"
     CDS             13168..>14892
                     /locus_tag="VMZ53_25190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31830.1"
                     /translation="MRQTAAAFLILAFAASRAASSASDEWEEEGEFMPPPPFALVDPS
                     IGGVKPAPASSPFLASSRIAVLRDRTLVSDEDSGTLILADSYGASMAQVAIGRDAGLL
                     AVDPIAKRAYVADRHGDRIVVVDVGETLTVAATWKTPAEPYGVALAPDRKTVLVTTIA
                     DRTLVAFDASSGREQWRAALDAEPRGIAIAPDGKRALVASVTSGALQEVALDGPHVAV
                     RKALAVTSRDQRAKGAFAVAFLGSSVAITAFVNDTPVTPFPDGDHYGGSSEPPIAPQL
                     AFIGTGDQQARAITNIVEPRAIAWDGARDVLYVGGMATDKLVVISHASQVDVAFASEA
                     PLGRRCGADGIAVDDHGNVRVWCAFSRSIARFDAKKNGALAKVEHGPELTTSTMDETR
                     HAGFVLFHTANAHVSSFGGMSCGSCHIDGRADGVSWHIRGRELQTPVLAGRISGSGPF
                     KWDGTAKTLQRSLRETIDRLGGDGLSKKQLGQLVTFVETMPAIRTPTRDVAAVARGKQ
                     LFQSPAVGCTDCHDGAAFTDSARHRFGGAAFDTPGLGALAASAPYFHDGSAPTLDAVL
                     RERGSVHGM"
BASE COUNT         2618 a         4856 c         4939 g         2479 t
ORIGIN      
        1 aaggcgctgt tcgagctcta cgacgcgttc ggctctgcat cgctcgacac gacgctatgg
       61 acgaacgtgc gcggctcgac gctgaacaac gggcgcctcg tcgtcggtgg cggcggccag
      121 acggacaatg gcgtgatttc gaagatgtcg tttggcgtca acaacgccgt cgacttcatc
      181 atcgtcgcgt cgcaggccac gaccgcaatg cctggctttt gggcgggctt cgagggcagc
      241 acgacggatg ggcctccgta cacgatctgg tggacgctcg acacgtcgaa catgatcgcg
      301 ccttcgtaca agatcacgac ggcgagcacc gactatcgcg gcacgccgtt cgcgttcacg
      361 acggccgcgc actactacag cgtcgagcac tacggcaaag ggacgatgta ccgctacgac
      421 gacgttccgt acgacacgca agcgtatgac gtcgcgtaca cgtcgccgct cttcatccgg
      481 ctgtggaact acaactcgtc gccgagcgtt gcgtacgaca tggttcgcgt gcggcaagca
      541 gtcgatccgc cgccggccgt cgtcgtcggt gcgcccgtcg cgaagccgta gtcagaacga
      601 gcgcggatcg tcgctgatga agagcgggaa cttcgccgtg cccgtcaggt gcagccgcgt
      661 cggcgcgcca tcgcgatagg tcatcgtcgc gacgccttcg ggatccggct cgcggatgcc
      721 accgccgaac acggcgacca tctcgtcgag ctcgagaacg gcctcgcgaa accgcagcga
      781 atcgagcatc gctggcgtgc gtcctgcgag acccgtgcga acgaggagct cgcgttgcgc
      841 tgcatctgct tcgagcgggc gcgactcgct catcgcatcg atgcccgacg agacaaacgc
      901 atgagcggga tcgatgacgg ccgcgccgct gtcgtcgcgc agcacgaacg ggatgccgtc
      961 ttgcaccgtc gcaagcgtgc gcagcatgct gttgccgacc atcgcgtcga tggacacgtc
     1021 gtagtagacg cagagtcgct tggacagcgg cgcttcgatc gcgcgatcgc gaaacggtcg
     1081 aacgtggccg acgatcttcg cgaacatgtt ctcgggcatg tcacgcacgc ggaacgtggg
     1141 agctttgcgc atccacttgc ggtgcttccg atccgcgaag tagcggccga ccgcgcccgg
     1201 gatccggacg aacgcggcga agagatcgtc gaatctgact ccgctcatcc gcgcttgtgg
     1261 gcggtgagcc aactgaagat gtcgtcgatc gcgttcgcgt tgaacagcgg gatgtggctc
     1321 gagccttcca tcgtccacag atcgacggca acacccgacg ggcaaccgct cgttgtctcg
     1381 ccgtgcgtct cggcgcctgc gacctgcgag tcgagatcga gcgtgaccgt cgacgtgcgt
     1441 gtcgtgccgc agccgtcgtg cgtcgcccat tgcgagacac tcgcgaccgc gcccgtgcca
     1501 ccgctcgcga atgggacggt cgcgtcggca gtgccgtgca tgtggagcac gttgacgggt
     1561 ttgtccggcg tgcacgccga ggcattcgtc gctgcgttgc ccgcgagcga agcgaccgcc
     1621 gcgatcacat ccgcgcgctc gcacgccatg cgataggaca tgtagccgcc gttcgagtgg
     1681 ccgatcagat agatctggtt cttgtcgatc ggccagtcgg ccacgacgtc gtcgatgagc
     1741 ccgccgatgt acgcgacgtc gtcgggattc ttgccttcga agtcgcagca cgccggatcg
     1801 gcattccaga attgctgacc gctgctgttc acggtgccgt cgggcgcgag cacgagtgcg
     1861 cggtccgaat tgacgagcgc cgacgcattg aagaacgcat attgcgcgaa gccgttcgcg
     1921 ccgaagccgt gcaagatgac gacgagcgga tacttcttgc cctccgtcag cgttgcaggc
     1981 gtgcgcaggt cgaccgggcg gtcgccgccg taggtcttcg tcggcggagc gtcgtccgat
     2041 gagccgcaag ccgcaagaag catcacgaga gccagtccgc gcatggccgg agactagcag
     2101 ggccactaca atgcaggcag tgccaccgcg cctcgtcgtg atcgtcggac ccaccggcgc
     2161 cggaaagacg cggctctccc tcgagctggc gatgcgcgtc ggcggcgagg tgatctcgtg
     2221 cgactcgcag caggtctacc tcgggatgga catcggcacg ggcaaggtca ctgccgacga
     2281 gcggtcgcgc gtgccgcatc atctgctcga tgtcgtgaag cccgaccaag agatgacgat
     2341 ggcgcggttc atcgacatgg ccgacgcggc ggtggccgag gtcgcgtcgc gcggcaagcc
     2401 cgtgatcgtc tgcggcggga cgggcctcta cgtgcgcggc ctgttgctcg gtctcttcga
     2461 ggggccaccc gccgacaccg agctgcgcaa ggagatccag gcgatgggca tcgccgcgat
     2521 ccgcgacgag ctcgagcgcg tcgatcccaa agccgcgtcg aagatcgacc gcaacgacga
     2581 gaagcggatg gtccgcgcgc tcgaggtcta tcgcctcacc ggtgagccga tgagcgcgca
     2641 tcaggcgcgt cacgaccaca agacgatggc gcgtcgctac gacgcgaagc tcgttggcct
     2701 cgcgccggag cgagagcagc tctatcgcca gatcgacgcc cgcgtcgacg agatgatggc
     2761 agccggcttc gagcaggaag tcgccgccct gcgggccgcc ggctacgcgc caccgctgcg
     2821 gtcgcagcag gcgatcggct acgacgagct gcatcaggtc gccgccggag cgctcgaccg
     2881 tggtcgggcc atcgagctga tcaaaagaaa ttcacgtcat tacgcacggc gtcagctgtc
     2941 ctggtaccgg cctgatcaga cgatcacctg gtacgcgtcc ccggccgccg ttgacctcgc
     3001 cgagttggag cggtacctag cagggctcta agtgtcgacg accgaagctc cgccgcagct
     3061 gaccgaccgg atgattctcg agttcgagcg tccgatcatc gatctcgaaa agaagatcag
     3121 cgagctgcgc ggcctctcga ccgacaccgt cgacttctcg gccgagattc gtaagctcga
     3181 gcagaaggcg cgcaggctcc agaaggaagt cttcgcggac ctcagcgcgc agcagaaagt
     3241 gcagctcgcg cgtcacccgg cgcggccgta catgaacgac tacatcggtc tgctcatgga
     3301 cgacttcgtc gagctgcacg gcgaccgcag cttccgcgat gacccggcga tcgtcggcgg
     3361 gctcgcgatg ttcgacgagt gggaagtcct cgtgctcggc caccagaagg gccgcaacac
     3421 gaaggacaac atgcatcgca acttcggcat ggcgcggccc gagggctatc gcaaggcgac
     3481 gcgcctcatg cgcatggcgt cgcggttccg ccggccgatc atctgcttca tcgatacgcc
     3541 cggcgcgtat cccggcctcg gcgccgagga gcgcggccaa gccgaagcga tcgcgaaggc
     3601 gctgcaggtg atggcgagcc tcgagtgccc gctgctctcg atcgtgatcg gcgaaggcgg
     3661 cagcggtggc gcgctcgccc tcggcgtgac agatcgcatc ctgatgctcg agtactcgat
     3721 ctactcggtg atctcgcccg aaggctgcgc gtcgatcctg tggcgcgacc cggcgaagat
     3781 cgcggaagcc gctacgcagc tgaagctgac cgcgccggat ctgctgcacc tcggcatctg
     3841 cgacgagatc attcccgaag ccgcgggtgg cgcacatcgc tcgcctgcgc agactgccgc
     3901 caagatgcgg atggcgatca agaagcacct caaagagctc gtcgagcttc cgcccgcaga
     3961 gctcgtcgaa cggcgctatc aaaagttccg caagatgggc gcgttcatcg agtcgccgtc
     4021 gtagcgagcg atgtactacg agctggcgct catctcggtc gccgtcgccg gcggatactg
     4081 gggctggtat ttcatccgtc accagacgac gcggctctac ggcatgctgc agctcggcgc
     4141 cgcgctgctg tcgttggtag gcctcctcgg tcgcaagatc ggtgagagct cgctggggat
     4201 cgcaggcgca gtcggcgtcg gcgcgggcgt gtgcttgctc gtgctcggtc cgctcgtccg
     4261 cgggttcgcg cggcgcctcg ccggtgccga acgtttcgac gccgcgaagc gcatgttcga
     4321 cgtcgccgag atcctcgcgc cgggttccgg cgtcgacgac gaaaagcttc tgctcgccgc
     4381 gatgcgcgag atccgcgacg gcaacatcga gacgacggtc gaggcactga ccgccgcgaa
     4441 gcagcgtgcg cccgcggatg ctcggctcgc gatcgacgag cgcatcgcca tgctctatct
     4501 cgcggcgtac cgctgggacg aagcgattac ccacgccgag gcgcatctgt tcggcgcgat
     4561 tcccgcgatg ggcgaagcga gctccagcca cgcggcgctg cgccgcgcac tcggcatcgc
     4621 gcccgccgtc tgggtcgagc tgctcggtgc gtacggttac gtcggcgatc tcgatcgcgc
     4681 cgcgtcgatg ctcgctcgcc tcgaagaagt gtgcgcgggt cgtccggacg cgagcgtgtg
     4741 gctgcatcgc gggcgcctga tcgcgctcgc gctcgccggt cgcgtcagcg ccgtgcaggc
     4801 gctcgtcgag ccgcggcggt cgcgccacat gaagccggct gcgcgcacgt actggctcgg
     4861 cgtggcgtac gaacgtcacg gcgagatcgc agcggccgaa gcagcgtacg caaaggcgcg
     4921 ctctcgctcg cgcggccggc cgcgcatctt gatcgatcaa gcgctcgcac gattgcccgt
     4981 cgctaaacct gtcgagctga gcacggaagc gagcgcgatc atcgagcgcg tcgaggccac
     5041 gccgcccccg gccgtcaccg agcgcatgca tcggcaagga ccgaccgcga cgcgcatcct
     5101 cgccgccgcg atgatcgtca ccgccgccac gatcgcgctc acgctcggca actccgccga
     5161 cctcggcgtg ttgatccgag acggcgcgct cgtccacacg ctcgtcaaag cagggcagtg
     5221 gtggcggctg ttcaccagca tcttcgtcca cgcaggtggc ttgcacttcc tcgtcaacct
     5281 cgtcggtctc tggatgctcg gccggctcgc cgaagagctg ttcgggtcgt ggcgaatggc
     5341 ggcgatcttc gcgctcgcgg gcctcggcgg tgcggtagcg agcctgctcg caacgaacgc
     5401 gaatatctcg accggtgcgt cgggcgcgat cctcggcttg ctaggtgcgg tcttcgcgga
     5461 gctcaccgtg catcgcaaga agcatcggct tgcttggacg cgcggcgtgt ggggcagcct
     5521 cgcggtcgtc gcgctcgcgc aggtcggcac cgacttcatg tatcgcctca ccgatcactg
     5581 ggcgcacgcg ggcggcctcg tcgtcggtgc gctcgcaggc ctggcgttct cgccgaacct
     5641 cgagtggaag aacgcacgcg ttcacgccgc gcgcgtcatc acggccatct tcgcagcggc
     5701 cgtcattacg agcttcgcgt tcacggttcg cacatcgatc gtcgacagcc tcacggcatc
     5761 gccggagttc gccgtcgagc tcgtgagccc gtacgacaag atgccggcga ccggaaacat
     5821 cgatgcctat atttccggca ccgcaaatcg cgcgaaggag aacttcagca tcgagcgcgt
     5881 cgaatccgcg cccgatacgc tcgtcacgct gccggccggc tggactggcc aggagatgat
     5941 cggctggctg ccgtcggagc tcggcgacga ccagaaggtc cgcatcatcg tggcgcgcgc
     6001 gcccgaccgc gtcctcacgc tctacctgcc cgaatccgtg gtccgcgggg cacccgaata
     6061 tttcacctgg ctgattcctc ggctgtaagc ttcgcttcgt gcgtatcgcg ctcgtgctcg
     6121 tgctcgtcgc aacggccagc gccgcggctg atccactcgg cgactgctac gacgagctcg
     6181 acgcgcgtca catcacgtac aagaagacga agaaatccgg catcaagaaa gccgtcgaga
     6241 tcaccggcac gctcggcggc gtcgaggtct cgggcgccga gccgctcgtg atcgactgct
     6301 cgctcgccgt gtcactcgac gaagccggcc gctacatgaa ggcgctcggc atggagagcg
     6361 cgcacttcgc gtcggcgtac tcgcggcgca tggtccgcgg cacgaatcat ccgagcaagc
     6421 acagctacgg cctcgcgatc gacgtgtcgt cgttcaccgg tcccgatctc ggcacggtgc
     6481 gcgtcgacaa agactacgag cagggcctcg gcgacgcggt cgattgcgtt ggccagccgc
     6541 tcacgcaagg cggcgcgatc ctcaaggtgc tgcagtgtca gctggtgcgc tcgggtctgt
     6601 ttcatctggt gctgtcgccg gactacgacg atgctcacca tgatcacttt catctggaag
     6661 cgattccgtg gccggaccgt tcttcgcttc gctcttcctc gcccgcgatc cactgactcg
     6721 cagcgcgacg aataggatcg cgagcgacca cgcgagccag atgatcgccc acgtccacac
     6781 ggcccatgtc tgcgtcgtgc cgaacgtcga cgctgccatg ttgtgtgcgt cggacatgtt
     6841 gccggtgctc tgaccgttga cgacttgcga cgggcgcaac agcacgcgga tatcgaggag
     6901 cgcattcacg cacgactgcg cggcgaggaa atgcgcgaac agcacgcgcc accgattcgg
     6961 caacaggatc gccgcgagcg cgatcgccgc accgattgcc ggcacgatgt actgcccgaa
     7021 gcgatcgccg ttcggcgccg gcgcgatcac gagcaccgtc gtaccgacaa gcaacacgga
     7081 catgacgatc agcgcgttac gcgcggctcg cgcattgggc gtcacgacca gcaagaccgc
     7141 gccccacaga ggcgtgccca tgtagccggc ggcggcgatg atcgctgtgc caaacgatcc
     7201 gaccgacgtg tacgcgtacg cgagaccgct cgtgtcgcga tagatcacca tgcgatcgaa
     7261 gcccgagccc gtgatgatca tcgcgaggcc atgcgagagc tcgtgcagcc acgttgcgag
     7321 gaccttgaac ggatagagca gggagcccgc gaacggcagg ttccacagca ggagagacgc
     7381 gagcagcgcg atcgctagcg tccgcatctc gtgacccttg cggcgcatcg ctactctcgc
     7441 ggcggctcga ctcgtttagc gtctggcttc tcggtcggcc gcttctcggc gggctcgaag
     7501 atgccttcga acatctgcgt cgtgcgatgg cgaacggccg cgccgagctt cacgagatcc
     7561 tcgggatcga gtagctggcg caacgcctcg ctcggcttct tgatcgcgag cgccatcacg
     7621 tcgctgatcc ggaccgtcga cccgccacta cacacgacgc cgatcaaggc gccgcagagc
     7681 gcaccggcaa tcgcgcccat gatcggaatc aggagccacg aggggccgcg ctccggcagc
     7741 tctccgtgcg ggatgaccgc gatcaacatg aggcccgcgc tcgccgcggc atgcgcacga
     7801 gccgccatgt acatcgcgtg gcggcggcca ccgatcttgt cgaccgacgc atacgccgcg
     7861 acccgcccga ttccagcggc cgtgaagagc gccgcgactc ccgcgaacac ggtcgtcagc
     7921 cgcagaatgt gcatccagga ggcgtcgcga tcccccacgc cgagccgggc gaggagccaa
     7981 actccgccag tcaaagcagg tacttcgatg acggcgcccg taagaagacc tttgaaaaag
     8041 ctccaacgcg gcggaaaagc cgcgggaagt cgcgcgctta cgatgggagc tttcgagtcg
     8101 gccatagacg cggtccgagg aagaccgcat gcccgataac cgtagcatgt tcggagtctg
     8161 cgcctgcgct gcggcaagcc gtcgcgaggg ccgtacgtat acaatctcaa aggcgactaa
     8221 tggcgaacgc taatccctct aatcgtgcct atgcagcggc gcacttcgcg ctcgagctcg
     8281 atggcaagga agacgtcgga atgttccgat cggtcgaggg cggcggcgtc aaggcggacg
     8341 tgatgacgta ccagcccgcc ccgagccagg agaacggcgg ttaccagaag tggcgccagc
     8401 tcggcaagcc gaagttcgac gacatcaagc tgcaagtcgg catgtcgatg gcgcagccgt
     8461 tctacgcatg gctcgccgac ttcttcgttg gcaaggcgac gcgcaagacc ggcgcgatcg
     8521 tcgccgcgga cttctattac aacgagcgcg ctcgtcgtga cttcaagggc gcgatgattc
     8581 gcgagctgac gttcccgaag ctcgatgccg ccgacaagaa cgccatctac atgaacatct
     8641 cggtgtgcgt cgaagacatc gagttcaaga agggcagcgg caccaagctc aaggtgccgg
     8701 agaagacgag cggccagaag ctgtggaccg catgcaactt cacgttcaag ctcgacggtc
     8761 tcgatgaagc ctgccgccgc gtgacgaaga tcgattcgtt cacggtgaag caagcggtcg
     8821 ccgagtatca cgccggtggc ttcaaggccg cgatcaagac gccgacggcg atggagtttc
     8881 cgaacctcat cttctacgtg cccgaagcgg acgcacagcc gttcatggag cacatgcaag
     8941 cgtcggtcgg cttcggcaag gacctcggca aaggcaaggt tcgcgatccg aagacgctca
     9001 acggcagcat ctcgacgttc gataacgaga accgccacct gctcgacgtc gagttcaagg
     9061 gcgcggacat cgtgagcgtt gcgccggaga agtcggagtc gaccaccgaa gaggtcaagc
     9121 tggtcaagat cgagctctac atcgagtcga tggagctcaa gtacccgagc gccgagctcg
     9181 agtaactagt tccggatcgc ttcgtcgcgg agcgagaacc agcgcttcac ggcctcttcc
     9241 ttcgcagctc ccagaagatc tgtgacggct tcgagctcgt cgggatcgtc gacctgggcg
     9301 aaagcctcca ccggggtcag cacgaacgcg agcacgtccc gcgcgctctt cggatccggc
     9361 tgataactgc tgctgtatag cgtttcgccc acgagccaca gtcgccttcg gggatcgacg
     9421 cggcaagttt tcggcggtag ggtccggcct atgccgcgct cgaaggttgc tgttctgcgg
     9481 gtccgtccgg accacgttct cgaggatatc gatcgcctct acgagcttgc cgacgtcaaa
     9541 gccgcgctca aaccaggcgc gcagacgatc ctcaaggaca acatctcgtg gcacttcccg
     9601 ttccccggcg cgaacaccac gccgtggcag ctcgaaggca ccgtccgtgc gctccacagc
     9661 cgcggctaca acgatctcgt ctgcgtccag aacaagaccg tcgtcaccga cgcattcaag
     9721 ggcgaagacc tcaacggcta cctcccgatc ttcaagcgct acgacatccc ggttcgctac
     9781 aacttcaagc cggaggacat gacgtggtcc gtctatcgcc cgaaggcgaa gatgaacgtc
     9841 ctcgacacga tcttcccgga aggcatccag atcccggacg cgttccacgg cacgaacatc
     9901 atccatctcc cgaccgtaaa gtgtcacatc tacacgacga ccaccggcgc gatgaagaac
     9961 gcgttcggcg gtctgctcaa cacgaagcgt cactacacgc actcgtggat ccacgagacg
    10021 ctcgtcgatc tgctcgcgat ccaaaaagag atccactccg gcctgttcgc ggtcatggac
    10081 ggcacgacag ccggcgatgg gccggggccg cgcacgatgc gaccggtgat caaagacgtg
    10141 atgctcgcgt cggatgatca ggtcgcgatc gatgcggtcg ccgcgtcgat gatgggcttc
    10201 gatccgatga cgctgaagta catctcgatg gccgacgagc agggtctcgg caatggccgt
    10261 cgcgagaaca tcgatgtggt cggtgacgaa gagctcgcga atcagcgctg gggcttctcg
    10321 gtcggcgaca acggtgcgtc gatggtcggc gacatcatgt ggttcgggcc gatgaagcgg
    10381 ttccagaagc tgttcttcca cacgccgctc gtgaacctgt tcgtgatggg gtccgaggcc
    10441 tatcacgact actaccggtg gccgcttcgc gaccggcgca tcttcgagca gtggcgcgac
    10501 tctacgacgt ggggtcgcct gttcgaccac tatcaagtac acggcacgct cgctccgcaa
    10561 gacgtcgcaa aggtcggctg aagatcggcg atactggacg ggtgcgcgtc gtcttgctca
    10621 ccctgctggt tggatgcggg ttcgagcatg gcgcgtgggg accggcatcg acagtcgatg
    10681 cgggcgatga cgtagacgcg gcgatcgacg ccgaggcaat ggtgatcgtc gatgcgccgc
    10741 ccgacccgcc gccgtcgacc aagagttggg tcgtgatcga gacgctgacg gtcccggtga
    10801 acgggcagag cgtgacgtcg cagacggcgc tggcgaacgg cgtgacgtat cacctgcgcg
    10861 cctcgggcac gtgggtgatt cagagtacgc cgggcacgca agccgacgcc gagttctggg
    10921 acttcacgaa tacggcgggt ccgcaggaag gtgtgaccgg cgtcgactgt gggctcgcgg
    10981 tagatgacac gaccgtcgat accaacaaga cgccgaagtg gggcgcgtat tccgcgagcc
    11041 acacgtacga gatcgactgg gtcggtgacg gcacgacgat cgtcgcgctc atgcatgacg
    11101 gtaactactc gaacaacacc ggctcgctgt cgctcgcgat cctggcgtat cagtagtttc
    11161 tcgaacactt ttgaataggc ggagcgtggc ccgacgttgt ggcgccatgc gttggcttcc
    11221 cgtgctcgtc gtcgccggct gtgcggagcc gagcgcgccg gctgtgcccc aggtctccgt
    11281 cgcgcccgat gtagagcagc caacgccgcg gtcgcctgca tgggatccgt gggcgtatcc
    11341 gcaggtggtt ccgccgaagc cgcccgagcc gtgggctctg tccacgagcg cagcctcgat
    11401 cgcggagccg gccaagcggc tcgtcacgtc gatccatggt gcgagtgaca tcgcggtgct
    11461 ggccgtgacg gaagatggtc gcgcggcggt cacggcggac attcgctcgg tccgtttctg
    11521 gccgcgcctc gatggcaagc acgagccggt cgttgtcgcg atgcggccgc ccgcagctct
    11581 tgcgatcgcg cgtgatggcg atgggctgct cgtcgcgggc ctcgatgcaa tcggccagct
    11641 cgagctcgtg cgtctcacgg ccgacggtga atcattgttg cgtgtggcgg cggcgagcgc
    11701 gcgaccgatt cgcgagatcc acgcgactgc gcgcggtgtg atcgctgtgc gcgacgatga
    11761 tgcgatcgcg ctctacgacg cggctggcga gctgcgcggt gagcttgctc cagtggcgcg
    11821 gcaacgcgtg cgtgcagtcg ccgtgcggca cgacgcagcg atcgcgcttg tcgagagcgg
    11881 gggaacgatg catggccagt ggctcgacgt cgatgcgacg ggggtgacac ggggccactc
    11941 gacaccggcg ttgccattcc gtgatccggc gttcgcgtta tcgacggatc atcaacggct
    12001 cgttggcacc gaccagcgag ggaatgtggt tgtcgtcgat gcgaataccg gccgcgcgct
    12061 gcaacaccgt tggaagaaca aagacgacgc tgcgctgcat gtggtcgggt ttatcgatcg
    12121 aagcgcgctc gcgatcgacg tcggtgcgcc attcgttgcg ctgcatgcca agcgcgattt
    12181 cgagcaatcc gaggagatag ccggccaaca cttcgtcacc gtcgacggag cggtcgtcgg
    12241 cgtcgaaggc gcggccctcg tgatcggtcg tcacggcaag ccggtgcagt acctcggcta
    12301 ccggatggcg tcgccgatta cggccgtgcc gcgcgacgac gggtttctca tcaccgatgg
    12361 cacggcgctc gcggagctcg gtcccgatct gcgtgcgcgc aagctgttca agatcgcgcc
    12421 gaaagacggc gagctgttca acacgcagct cgtggatgcc gatcacgtgg tcaccgcatt
    12481 cgcgcccggc cgcgacaatc cggtgggcta cgtattcgcc gacgtaagca aaggaaccgt
    12541 cacacctctc ggcgacggct acgccagctt cagccgcgag aacggcctcc ttgccatcca
    12601 gcagctaagc cgcatcgacg tgcgccgcta cgatcgcaag cagggcaagc taggtcccgc
    12661 gtcgacgctg catgtctcgt cgtgggttcc gttcgtgtgg ctcgattcga aacgcggaga
    12721 cttcgcgctc gtcacgctcg atttcgatgc gcgcgacgca gagctcgatg tctttcggct
    12781 gcgcgacgac gtggagcggc taaagacgcg gaacgtgaaa ccggccgagc gttggtggga
    12841 cgcccagaac atggacacgc tgctcggtga gacgtggcga gtgagccggc gacgaagcag
    12901 tccggacggc agcctcgtcg ccgaagtgag cacggatggc cggatgacgc tgcacgatgc
    12961 gacgggcgca gagcgctgga tggtcgcttc gggcggcgcg atggatgtgt tgtggacgcc
    13021 ggctggagag ctcgtcgcat tcggcgctgg gattgctcgc gtcgatatcg aaaccggtgc
    13081 gctgcgcgag cggcagtgtg gttggcaatt tggcttgtgg cccgcatcga ccacaccgat
    13141 cagcgcttcg gcgctctgtg aggcaccatg agacagacag cggccgcgtt cttgattctc
    13201 gcattcgcag cgagtcgtgc tgcctcgagt gcttccgacg agtgggaaga agagggtgag
    13261 ttcatgcccc cgccgccgtt cgcgctcgtc gatccgtcga tcggaggcgt gaagcctgcc
    13321 ccggcgtcat cgccattcct cgcgagctcg cgtatcgcgg tccttcgcga tcggacgctc
    13381 gtcagcgacg aggacagcgg cacgttgatc cttgccgact cgtacggcgc atcgatggcg
    13441 caggtagcga tcggccgcga cgcgggactg ttggcggtcg atccaatcgc gaagcgtgcc
    13501 tacgtggcgg atcgccatgg cgatcgcatt gtcgtggtcg acgtcggcga aacgctgacc
    13561 gttgctgcga cgtggaagac gccagccgag ccatacggcg tagcgctcgc gccagaccgc
    13621 aagacggtcc tggtgacgac gatcgcggat cggacactcg tcgcgttcga tgcttcgtcg
    13681 ggccgagagc aatggcgggc cgcgctcgac gccgagccgc gaggaatcgc cattgccccc
    13741 gacggcaagc gcgcgctcgt cgcgtcggtt acgagcggcg cactgcagga agtcgcgctc
    13801 gatgggccgc acgtcgcggt tcgcaaggcg ctggcagtca catcgcgcga ccagcgtgcg
    13861 aagggcgcct ttgcagtcgc gttcctcgga agctccgtcg cgatcacggc attcgtgaat
    13921 gacacaccgg tcacgccgtt cccagatggc gatcactacg gcggaagcag cgagccaccg
    13981 atcgcaccac agctcgcgtt catcgggacg ggcgatcagc aggcgagggc aatcaccaac
    14041 atcgtcgagc ctcgcgcgat cgcatgggac ggcgcccgcg acgtgctcta tgtcggcggc
    14101 atggcgacgg acaagctcgt cgtgatcagc cacgcttcgc aggtcgacgt cgcgttcgcg
    14161 tcggaggccc cgctcgggcg gcgttgcggc gcggatggga tcgccgtcga cgatcacggc
    14221 aacgttcgcg tgtggtgcgc gttctcgcga tcgatcgcgc gcttcgacgc gaagaagaac
    14281 ggtgcgctcg cgaaggtcga gcacgggccg gagctcacga cgtcgaccat ggacgagacg
    14341 cggcacgcag gcttcgtgct gtttcacacc gcgaacgctc acgtgtcatc gtttggcggc
    14401 atgtcgtgcg gcagctgcca catcgacggt cgcgccgatg gcgtctcgtg gcacatccgc
    14461 ggtcgcgagc tgcagacccc cgtgctcgcc ggtcgaatca gcggcagcgg tccgttcaag
    14521 tgggacggca ccgcgaagac gctgcagcgg agcttgcgcg agacgatcga ccggctcggc
    14581 ggcgacggac tctcgaagaa gcaactcggc cagctcgtga cgttcgtcga gacgatgccc
    14641 gcgattcgga cgccgacgcg agacgtcgcc gccgtcgcgc gcggtaagca gctgtttcaa
    14701 tcaccggccg tcggctgcac ggactgtcat gacggcgcag cgtttaccga tagtgcgcgc
    14761 catcgcttcg gcggcgccgc gttcgacacg cccggcctcg gagcactggc tgcgagcgcg
    14821 ccgtactttc acgatggcag cgcgccgacg ctcgacgccg tgctgcgcga acgaggcagc
    14881 gtgcacggaa tg
//
LOCUS       DATLBZ010000499        11510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5854877, whole genome shotgun sequence.
ACCESSION   DATLBZ010000499 DATLBZ010000000
VERSION     DATLBZ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5854877"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VMZ53_25195"
     CDS             <1..667
                     /locus_tag="VMZ53_25195"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS31831.1"
                     /translation="IVSWRVVKQTLLRPLPFYAIVSTLIGYTVLFVISKVGGAGVKPE
                     ALLRQIGGSHVVALAPALSALLFVAASGSATNAWLGSMGLTKQTLALDALGVDRRSYL
                     WAPAWLAAALCYLAVSALFALGMILGGLLVCRAHGITNGWELLTGDLLDPRPERLRYA
                     VRAAFLVWIYAWGIASDVVAKGGASKPEADAVTRGMTASVVACTLWVVGWELVTVLVV
                     FRL"
     gene            664..2244
                     /locus_tag="VMZ53_25200"
     CDS             664..2244
                     /locus_tag="VMZ53_25200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31832.1"
                     /translation="MKLAAIIGVAIVALHGAGFVYVANHSRGDELAVDISELKLDGGP
                     GLVRKHWTKTYRGGFTRDVGVTQLVGPFQDPAKPACTGRVVIGQSFLTDQLAPIMKAM
                     IDEQLRGMEVFPVGQYKSIQHLGLEWAHVENHLGDFAMFGKAGAPNGYIRATARVSFS
                     RVDVPVLVVLVPEVIATSTSPSPSASPQMHFRINARADLDFGNRVLDWVSDKVGADQL
                     ASRIARQQIDDVLVTTFAPPPPFDLGDGQTLQFTYCDAPVDIANNAYGALPFGVHIEG
                     HAGLLPPQFATSPTTSPSPSRALPSATTQLAIDLDLDALNAMLFELWRSGWLDKRLAE
                     VGLDARFNSDPIVTEYLSIRISPPKLALPPVIAPGSNGKLRLAADARTMIDDGASTVG
                     RVYGALDFTFGTSQTTTGTPQTTTGASPALAAAPKATSFTPSVDLGALELACEQRPNV
                     LVPCYGDLVAAMRDRGGEFHGALTDAFARLLADIFVNRRVGADGLPADLVITSASATL
                     GGTGTLHLELTGKLEPVN"
     gene            2371..3090
                     /locus_tag="VMZ53_25205"
     CDS             2371..3090
                     /locus_tag="VMZ53_25205"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUS31833.1"
                     /translation="MDRDGFREALLAIMERKVHWAWPHFGAGRVPADKLHIHFEQEYA
                     TYVRDFPMMVGWAYVQCPIAEVRRDLAENLYEEETGGVIAGKPHPELFLEYPRGLKMN
                     LSRFEHVELLPAARAYRAVLDEHTQRHGWAQAAAVTTIFVEGTDHERGELDPNAAKRP
                     APPLEQHPLVVHYGLPVEGLALTKAHRRVEGSHRAAAWRMILEHVPASEYAGVIDAME
                     RTLEAWRSYRDAVAAACGLSR"
     gene            3111..4076
                     /locus_tag="VMZ53_25210"
     CDS             3111..4076
                     /locus_tag="VMZ53_25210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31834.1"
                     /translation="MHRLALLVATVAACGGGAAQEPVKPIGIVPVPAVAPPPKPVCIR
                     APEDVAAITHATADETHVSYCIGEAVDQCFKLDLETGKLDLLAEPPAAQDKSLTPTGH
                     VETTNPELKVCNANGCKTLTPQVWPGAAPLHAATNGAVAVVMLGDAEAGKGYVDVYDA
                     GKAKKLATFKYARGEMKCGEVAMLGDVIYVSAIACKSPSGRGALYTTKGKKIANVGGK
                     GEFGTYGGAYTQLTDTTWAFLEENGNMIAVQDVAKGKVLKTVDVTQLFDNVRMGNPGE
                     SAIVRLSAGKLAVIGGTPANGSVAIVDPASGEVKVVKAPLCGSGG"
     gene            4211..5332
                     /locus_tag="VMZ53_25215"
     CDS             4211..5332
                     /locus_tag="VMZ53_25215"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS31835.1"
                     /translation="MPAAATSVERQGRTAIVRVRGDVVIPTVRALHGTLRGLQRRRDV
                     KKVVLSLGSTGKLDSSAVAVIELARRGLHRCGKDFELADLHDHHRAALELAPEVKAKP
                     QAKVEGPSFLERVGEYVLSLGSTIADLAILVGATLREAASVVARRRKLPAGSVSSFIL
                     LMGVDAVFIVGLLSFLLGMTMAFQGVVQLERFGAGVFVADMVGWSMVREFAPLMTAII
                     LTGRTGASIAAELGTMRVNLEIDALTAMGVNPVRFLVVPRLAALIFVQPALTLMGMFI
                     GISAGMLIASLMIDMSPMTFWLHIQQRVTIADFAYGIAKSLVFASIIGFAGTHLGMRA
                     TGDASSVGRATTRTVVVSVFLIIVTDAIFATVSSLLGAR"
     gene            5353..6165
                     /locus_tag="VMZ53_25220"
     CDS             5353..6165
                     /locus_tag="VMZ53_25220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS31836.1"
                     /translation="MSDARISIPEARISAGDVRVTMPGTESGPLLECVDVAVGYEKPV
                     LQHANIQIGRGEIVALLGGSGCGKSTLLRTLVGLVPPLAGQVMLFGQSMYELEPNARD
                     KLRKRIGIAFQQDALFSSMTIEDNVALPLRELTKLPGDIIKEMVRMRLALVGIAELGR
                     RLPANISGGQRKRAALARASILDPELIFCDEPTAGLDPVVAAEVDATLVKFRDALGSS
                     IVLVSHELESIRATANRGIMLARGAVAAIGTIDELTHSRDEAVHQFFHARNE"
     gene            6162..6989
                     /locus_tag="VMZ53_25225"
     CDS             6162..6989
                     /locus_tag="VMZ53_25225"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HUS31837.1"
                     /translation="MTTKQQKVRIGLFVVIAGLLLAVVLVTFAGLHFWKPRTKYRIVF
                     DQTVYGLEPGADVYVNGIRSGKVTDIRLAPDDLRKVEVTIAVKEGTPIHQDTKAVMQF
                     AGLTGLKVIDLRGGTLQSPKLAAGGRIEVGETILDKLEDRAMRMVDQTDDMLKNANSV
                     VKSAQQVVNNVNELTDPKALGEVIAQSRQAAANLSQLSQAMRGLVDDNRASIRASVTS
                     METATRNFSTMIDGNQVKAAVSDLRQASRTMKEMAREVRQKPSRLLFSKPEPDRKLP"
     gene            6986..7648
                     /locus_tag="VMZ53_25230"
     CDS             6986..7648
                     /locus_tag="VMZ53_25230"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HUS31838.1"
                     /translation="MKRALLSLVFAVSTAACAGKVPATRFYQLATPSTPTPTNNSGVT
                     LAVETLDTDNAYDDDRMVYRLTPYRLDYYNYHRWSATPGTLVGNFLEAAFEASGRFRN
                     VTREADAQAPVILGGRVLALEEVDKSKTSWVGHVVIELRLTDATTGDVVWSQQFDETE
                     PVKEQTPEGLAQALSRALNRIAQNALPMIAEHATQVAKANDAQKASEAKATTSRSARL
                     KP"
     gene            complement(7645..8421)
                     /locus_tag="VMZ53_25235"
     CDS             complement(7645..8421)
                     /locus_tag="VMZ53_25235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31839.1"
                     /translation="MSDDDDKKVKKLLDDAHQTGALSAKSLAALDVVDVGAQIQAGLG
                     VTIDDVAASEVVLLTMMPDDSQSIAAANNTDAVKDGHNFVLEALARSKQSGEVLAHTR
                     YLNGDVLFPYTTLANASQMTAANYNPCHGTPLFDQTVVLLGTVIAKAQELQQAGIAVR
                     TVTLIITDGGDYGSTRCRTSDVAAIVADMLAQENHIVAAMGINDGSTDFKKVFKSMGI
                     PDRWVLTPGNSASEIRRAFQVFSQSAVRATAGAQLGGFSN"
     gene            complement(8418..9464)
                     /locus_tag="VMZ53_25240"
     CDS             complement(8418..9464)
                     /locus_tag="VMZ53_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HUS31840.1"
                     /translation="MTLPIPTFFDPKRAADWSYRPDAAQLASAGAAWRAQHKLKPAAA
                     DETRVHLLLIDVQKDFCFPEGTLYVAGRSGKGAIDDSRRIAEFVYRNLASITDITTTL
                     DTHLAYQIFFPSFWLDKADQPVAAFTTITSAMIAAGEVRPNPAMAKWLCGGNYTWLCK
                     QALFYCQELERAGKYQLYLWPPHCLLGSDGHALAGVIHEARLFHAFTRVAQSNVEVKG
                     GNPLTENYSVLRPEVLSRFDGAALAQRNTQFIHTLLAADAVVIAGQAASHCVKSTIDD
                     LLTEIAAQDPKLAAKVYLMTDCMSAVTVPDGKGGLAADFTPQADAALQKFADAGMRLV
                     KSTDPITTWPGVKL"
     gene            complement(9477..11207)
                     /locus_tag="VMZ53_25245"
     CDS             complement(9477..11207)
                     /locus_tag="VMZ53_25245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31841.1"
                     /translation="MTARHVWIDGTRVALDPAALIGQGGEAEVYDLGDGRVLKWWKPA
                     DHPDFDGLPEQQAAAERRLAEQPAKLRALPGNLPSAVIAPSGLALASKKASARLGAAA
                     IAGGITGAAAVQGECVGYVMPKITGVPLHSYGEPRWRRENPVDGADVVAALVALHDAI
                     AGLHRAGVVIGDCNDLNVLVDGRRVHLIDVDSYQYGGFACSMFSERFVDPRLCDAQQL
                     VPVRPHDADSDWFAFAVMAFRSLLGVGPWGGVAKSCPATQRALRRVSVLASDVVYPRA
                     AKPLQMLPDDLLALFRDWFERDVRGEYPRVALERLRLQRCKSCGDEHARVRCPSCQTR
                     AFVPPSVVHGRLRYQVIATSEVAPAATHGVPVQPMNESSFMQSMKGTAVWLDGGALMR
                     RTRVGSERIGSVLAGQTRAWVGDSMGVGFYRAGGYAVGFVFKPDRGGLDDRVALPKLR
                     GRLLDAHAVIAGGAQTGADARAWLFFTTEQGTSCVVVDANARVVASEMLVDATWLAGV
                     GGACAAGNFLFVPTDDGVARIEILQGAIACTRVFAETAPLVGAADRLSLSPGGLDVVR
                     RRDAIRMQLS"
     gene            complement(11204..>11510)
                     /locus_tag="VMZ53_25250"
     CDS             complement(11204..>11510)
                     /locus_tag="VMZ53_25250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31842.1"
                     /translation="MCPRGHGHHGTIIVGTDGAGEIVGGIERFAQRRYLDHPDALRRE
                     LSLLAKPQERIDWDERRVIRTPAMVQDDCAIGVLHIERVGIATSATGVTGVEREVLS"
BASE COUNT         1963 a         3892 c         3726 g         1929 t
ORIGIN      
        1 gatcgtgagc tggcgagtcg tcaagcagac actgctgcgg ccgctgccgt tctacgcgat
       61 cgtctcgacg ctgatcggct acacggtgct gttcgtgatc tccaaagtcg gcggtgcagg
      121 cgtaaagccc gaagcgttgc ttcgccagat cggcggcagt cacgtcgtcg cgctcgcacc
      181 agctctctcg gcgctgctgt tcgtcgcggc atcgggcagc gccacgaatg cgtggctcgg
      241 ctcgatgggc ctgacgaagc agacgctcgc gctcgacgcg ctcggtgtcg atcgacgttc
      301 gtacctctgg gcgccggcat ggctcgccgc cgcgctctgc tacctcgcgg tctccgcgct
      361 gttcgcgctc gggatgatcc tcggcgggct gctcgtctgc cgcgcgcacg ggatcacgaa
      421 tggctgggag ctgcttaccg gcgatttgct ggacccgcgc cccgaacgcc tgcgctacgc
      481 ggtccgcgcc gcatttctcg tgtggatcta cgcgtggggc atcgcgtccg acgtcgttgc
      541 gaagggcggt gcatcgaagc ccgaggccga tgccgtcacg cgcggtatga ccgcgagcgt
      601 cgtggcgtgc acgctgtggg tcgtcggctg ggagctcgtc acggtgctcg tggtgttccg
      661 cctttgaagc tcgccgccat tatcggcgtc gcgatcgtcg cgctgcacgg cgcgggcttc
      721 gtctatgtcg cgaatcattc gcgcggcgac gagcttgcgg tcgatatctc cgagctgaag
      781 ctcgatggtg gtccgggcct cgtgcgcaaa cactggacga agacgtatcg cggcggcttc
      841 acgcgcgacg tcggtgtcac gcagctcgtc ggtccattcc aagatccggc gaagcccgcg
      901 tgtacgggcc gcgtcgtcat cggccaatcg ttcctcaccg atcaactcgc accgatcatg
      961 aaggcgatga tcgacgagca gctgcgcggc atggaagtct tccccgtcgg ccaatacaag
     1021 agcatccagc acctcggcct cgagtgggcg cacgtcgaga accacctcgg tgacttcgcg
     1081 atgttcggca aggcgggcgc gcccaacggc tacatccgcg caaccgcgcg cgtgagcttc
     1141 tcgcgtgtcg atgttcccgt gctcgtggtg ctagtcccgg aggtgatcgc gacaagcaca
     1201 tcgccgtcgc caagcgcatc gccgcaaatg cactttcgca tcaacgcgcg cgcggacctc
     1261 gacttcggca accgcgtgct cgactgggtc tccgacaaag tcggcgccga ccagctcgcg
     1321 tcgaggatcg cgcgccagca gatcgacgac gttctcgtca cgacgttcgc gccgccgcca
     1381 ccattcgatc tcggcgatgg ccaaacgctg cagttcacgt actgcgacgc gccggtggac
     1441 atcgcgaaca acgcgtacgg cgcgctgccg ttcggagtgc acatcgaagg ccatgccggc
     1501 ctgctgccgc cgcagttcgc cacatcgccg acgacgagcc catcgccgtc gcgcgcgctg
     1561 ccctccgcga cgacgcagct cgcgatcgac ctcgatctcg acgctctcaa cgccatgttg
     1621 ttcgagctct ggcgcagcgg ctggctcgac aagcgactgg ccgaggtcgg tctcgatgct
     1681 cggttcaaca gcgatccgat cgtgacggag tacctgtcga tccggatatc gccaccgaag
     1741 ctcgcactac caccggtgat cgcgcccggt tcgaacggca agctgcgcct cgccgccgac
     1801 gcccgcacca tgatcgacga cggcgcatcg accgtcggcc gcgtttacgg cgccctcgac
     1861 ttcacgtttg gcacatcgca aacgacaacc ggcacaccgc agacgacgac cggtgctagc
     1921 cccgcgctcg cagccgctcc caaggcgacg agctttacac cgagcgtcga cctcggcgcg
     1981 ttggagctag cgtgcgagca gcggccgaac gtcttggtgc cgtgttacgg cgatctcgta
     2041 gcggcgatgc gcgatcgcgg gggagagttt cacggcgcgt tgaccgatgc tttcgcgcgc
     2101 ttgctcgccg atatcttcgt gaatcgccgc gtcggggcag atggcttacc ggctgatctc
     2161 gtgatcacgt cggcatctgc cactcttggt ggcacaggca cgctgcatct ggaattgacc
     2221 ggcaagctcg agcccgtgaa ctgacgactg cgtgtttcgc ctgcaagcta gtgcatggcg
     2281 aacacatcgt ttcggcgaat tggtcacgcc aaagcgagac atcacgactt ccaaacttgc
     2341 ccgtttcggc cgatcgccta cgatctacag gtggaccgag acggctttag ggaagcgctc
     2401 ctcgcgatca tggagcgtaa ggtgcattgg gcgtggccgc acttcggggc gggtcgcgtg
     2461 ccggcggaca agctgcacat tcacttcgag caggagtacg cgacgtacgt ccgcgacttc
     2521 cccatgatgg tcggctgggc gtacgtgcag tgtccgatcg ccgaggtccg acgcgacctc
     2581 gccgagaacc tctatgaaga agagaccggc ggcgtgatcg cgggcaagcc gcatcccgag
     2641 ctgttcctcg aatatccgcg cggcctcaag atgaatctgt cgcggttcga gcacgtcgag
     2701 ctgttgcctg cggcgcgcgc ctatcgcgcg gtactcgacg agcacacgca gcggcatggc
     2761 tgggcacagg cggcagccgt cacgacgatc tttgtcgagg gcaccgacca cgagcgcggc
     2821 gagctcgatc caaatgctgc gaagcgaccc gcaccgccac tcgagcagca tccgctcgtc
     2881 gtgcattatg ggctacccgt cgagggcttg gcgctgacga aggcgcatcg ccgcgtcgag
     2941 ggcagtcatc gtgccgcagc gtggcgcatg atcttggagc acgttcctgc gagcgagtac
     3001 gccggcgtca tcgacgcgat ggagcgaacg ctcgaggcgt ggcgcagcta tcgtgatgcg
     3061 gtagccgctg cctgcgggct cagccgctga tcgtgtaaca tccacggttg atgcaccggt
     3121 tggctctcct tgtcgcgacg gtcgccgcgt gcggaggtgg cgccgcgcag gagcccgtca
     3181 agccgatcgg tatcgtcccc gtgcctgcgg tcgcgccgcc tccgaagccc gtctgcattc
     3241 gcgcgcccga ggacgtcgcc gcgatcacgc atgcgaccgc cgatgaaacg cacgtgagct
     3301 actgcatcgg tgaagctgtc gatcagtgct tcaagctcga tctcgagacc ggcaagctcg
     3361 acctgctcgc cgagccgccg gctgcgcaag acaagtcgct cacgccgacg gggcacgtcg
     3421 agacaacgaa tcccgagctg aaggtctgca acgcgaatgg ctgcaagacg ctcacgccgc
     3481 aggtgtggcc cggcgctgcg ccccttcatg cagcgacgaa tggtgctgtc gcggttgtga
     3541 tgctgggcga cgccgaagcc ggcaagggct acgtcgacgt gtacgacgcg ggcaaggcca
     3601 agaagctcgc caccttcaag tacgcacgcg gcgagatgaa gtgcggcgag gtcgcgatgc
     3661 tcggcgacgt gatctacgtc agcgcgattg cgtgcaagag cccgtcggga cgtggcgcgc
     3721 tgtacacgac caagggcaag aagatcgcga acgttggtgg caagggcgaa ttcggcacct
     3781 atggcggcgc gtacacgcaa ctgaccgaca cgacgtgggc ctttctcgag gagaacggca
     3841 acatgatcgc cgtgcaagat gtcgcgaagg gcaaggtctt gaagacagtc gacgtcacgc
     3901 agctcttcga caatgtgcgg atgggtaatc ctggcgagtc ggcgatcgtg cgtttgagcg
     3961 cgggcaagct ggcggtcatt ggcggcacac ctgcgaacgg cagtgtggcg atcgtggatc
     4021 cggcgtcggg tgaggtgaag gtcgtcaaag cgccgctctg cggcagcggc ggctagctcc
     4081 cggagcggtt gcggtagctt ctgcgcgcgg cgtgcgtcca cgcacgggca aagtgggcgc
     4141 caagtggaca gtcacttcgt gtcttcggcg ctctcggatt gtcctcgatc ttgcagttga
     4201 ggtgaccgcg atgccggccg ctgccacctc cgtcgagcgc caagggcgaa cggccatcgt
     4261 gcgtgtgcgc ggcgacgtgg tgatcccgac cgtacgtgcg ctccacggca cgcttcgcgg
     4321 cctccagagg cgccgcgacg tcaagaaggt cgtgctctcg ctcgggagca ccggcaagct
     4381 cgactcctcg gcggtcgcgg tcatcgagct cgcgcggcgc ggcctccatc gctgcggcaa
     4441 ggacttcgag ctcgccgact tgcacgacca tcatcgtgcc gccctcgagc ttgcccccga
     4501 ggtaaaggcg aagccgcagg ccaaggtaga aggcccgagc ttcctcgaac gcgtcggcga
     4561 gtacgtgctc tctctcggct cgacgattgc cgacctcgcg atcctcgtcg gagcgacgct
     4621 gcgcgaggcg gcatcggttg ttgcacgccg gcgcaagctg cctgctgggt cggtgtcgag
     4681 cttcatcttg ctgatgggcg tcgacgcggt gttcatcgtc ggcctcttga gcttcttgct
     4741 cggcatgacg atggcgttcc aaggcgtcgt ccagctcgag aggttcggtg ccggcgtgtt
     4801 cgtcgcggac atggtcggct ggtcgatggt tcgcgagttc gcgccgctga tgacggcgat
     4861 catcctgacg ggtcgcaccg gcgcttcgat cgcggccgag ctcggcacga tgcgcgtcaa
     4921 cctcgagatc gatgcgctca cggcgatggg cgtcaatccc gtccgcttcc tggtggtgcc
     4981 gcgcctcgct gcgctgatct tcgtgcagcc cgcgctgacg ctcatgggca tgttcattgg
     5041 catctcggcc ggcatgctga tcgcgtcact gatgatcgac atgtcgccga tgacgttctg
     5101 gctgcacatc cagcagcgcg tcaccatcgc cgacttcgca tacggcatcg cgaagagtct
     5161 cgtgttcgca tcgattatcg gcttcgcggg cacccacctc ggcatgcgcg cgaccggcga
     5221 tgcgagcagc gtgggtcgcg ccacgacccg caccgtcgtc gtcagcgtgt tcctcatcat
     5281 cgttacggac gcgatcttcg cgacggtctc gtcgctgctg ggggcacgat gaggaccgac
     5341 gcaaggacct cgatgtccga cgcgcggatc tcgatccccg aggcccggat ctctgcgggc
     5401 gacgtgcgag tcacgatgcc gggcaccgag agcgggccgc tactcgagtg cgtcgacgtc
     5461 gccgtcggct acgagaagcc ggttctgcaa cacgccaaca tccagatcgg tcgcggcgag
     5521 atcgtcgcgc tgctcggtgg atcgggttgc ggcaagtcga cgctactccg cacgctcgtc
     5581 ggtctcgttc cgccgcttgc cggccaggtg atgttgttcg gccagtcgat gtacgagctc
     5641 gagcccaacg cgcgcgacaa gctccgcaag cgcatcggca tcgcattcca gcaggacgcg
     5701 ctgttctcgt cgatgacgat cgaggacaac gtcgcgctcc cgctgcgcga gctcacgaag
     5761 cttcccggcg acatcatcaa agagatggtc cggatgcgcc tcgctctcgt cgggatcgcg
     5821 gagctagggc gccgcctgcc cgccaacatc tccggtggcc agcgcaaacg cgccgcgctt
     5881 gctcgcgcgt cgatcctcga tcccgagctg atcttctgcg acgagccgac cgctgggctc
     5941 gatcctgttg tcgcggccga agtcgacgcc acgctcgtca aattccgcga cgccctcggc
     6001 agctcgatcg tcctcgtgag ccacgagctc gagagcattc gtgcgaccgc caaccgcgga
     6061 atcatgctcg cgcgtggcgc cgtcgctgcg atcggcacca tcgacgagct gacgcacagc
     6121 cgcgacgaag cagttcatca attctttcat gcgaggaacg aatgacgacc aagcagcaaa
     6181 aggttcgtat cggcttgttc gtggtgatcg ccggactgct gctcgcggtc gtgctcgtga
     6241 cgttcgcggg tctgcacttc tggaagccac ggacgaagta ccgcatcgtg ttcgaccaaa
     6301 cggtctatgg cctcgagccc ggcgccgacg tctacgtcaa cggcatccgc tccggcaaag
     6361 tcacggacat ccggctcgcg cctgacgatc tgcgcaaagt cgaggtcacg atcgcggtca
     6421 aggaaggcac gccgatccac caagacacga aagccgtcat gcagttcgcg ggtctcaccg
     6481 gcctcaaggt gatcgacctc cgcggcggca cgttgcagtc cccgaagctc gcagccggtg
     6541 gccggatcga agtcggcgaa acgatcctcg acaagctcga agaccgcgcc atgagaatgg
     6601 tcgaccaaac cgacgacatg ctgaagaacg cgaacagcgt cgtgaaaagc gcacagcagg
     6661 tcgtcaacaa cgtcaacgag ctgaccgatc ccaaggccct cggcgaggtc atcgcgcagt
     6721 cgcgtcaagc ggccgcgaac ctctcgcagc tgtcgcaagc gatgcgtggc ctcgtcgacg
     6781 acaaccgcgc gtcgatccgc gcctccgtca cctcgatgga gaccgcgacg cgcaacttct
     6841 cgacgatgat cgacggcaac caagtcaaag ccgcggtctc cgacctgcgc caagcaagcc
     6901 gcacgatgaa agagatggcg cgcgaagtcc ggcaaaagcc gtcgcgcctg ctcttctcga
     6961 aacccgagcc cgacaggaag ctgccatgaa gcgcgcgctg ctctccctcg tcttcgccgt
     7021 ctccaccgcc gcgtgcgcag gcaaagtgcc cgccacgcgc ttctaccagc tcgcgacgcc
     7081 gtcgacgccg acgccgacga acaacagcgg cgtgacgctg gccgtcgaga cgctcgacac
     7141 cgacaacgcg tacgacgacg accgcatggt ctaccgcctc acgccgtacc gtctcgacta
     7201 ctacaactac catcgctgga gcgcgacgcc ggggacgctg gtcggcaact tcctggaagc
     7261 cgcgttcgaa gccagtggcc ggttccgcaa cgtcacgcgc gaagcggatg cgcaagctcc
     7321 ggtcatcctc ggcggccgcg tgctcgcgct cgaggaagtc gacaagtcga agacgagctg
     7381 ggtcggccac gtcgtgatcg agctgcgcct caccgacgca acgaccggcg atgtcgtgtg
     7441 gtcgcaacag ttcgacgaga cggagccggt caaagagcaa acgccggaag gcctggcgca
     7501 agcgctctcg agggcgctca accggatcgc gcagaacgcg ctgccgatga tcgcggagca
     7561 cgcgacgcag gtcgccaaag ccaacgatgc gcagaaagca tccgaggcga aggcaacgac
     7621 ctcgcgctcc gcgcggctga agccctagtt cgagaagccg ccgagctgcg cgccggctgt
     7681 cgcgcgaacg gccgattggc tgaacacctg gaacgcgcgc cggatctccg acgcagaatt
     7741 gcccggtgtc agcacccagc ggtccgggat gcccatcgac ttgaacacct tcttgaagtc
     7801 ggtcgagcca tcgttgatcc ccatcgcggc gacgatgtgg ttctcctgcg cgagcatgtc
     7861 cgcgacgatc gcagcgacgt cggaggtgcg gcagcgcgtc gagccgtagt cgccgccgtc
     7921 ggtgatgatc agcgtgacgg tacggaccgc gatgcccgct tgctgtagct cctgcgcttt
     7981 cgcgatcacg gtgccgagca gcacgaccgt ttgatcgaac agaggcgtgc cgtgacacgg
     8041 gttgtagttc gccgccgtca tctgcgaagc gttggcgagc gtcgtgtacg ggaacaggac
     8101 gtcgccgttc aagtagcgcg tgtgcgcgag cacttcgccc gattgcttgc tgcgcgccag
     8161 cgcttcgagc acgaagttgt ggccgtcttt caccgcgtcg gtgttgttgg ccgccgcgat
     8221 cgactgcgag tcgtccggca tcatcgtgag cagcacgacc tcgctcgctg cgacgtcgtc
     8281 gatcgtgacg ccgaggccag cctggatctg cgcacccacg tcgacaacgt cgagcgcggc
     8341 aagcgacttt gcggacagcg cgccggtctg gtgcgcgtcg tcgaggagct tctttacttt
     8401 cttgtcgtcg tcatcgctca tagcttcact ccgggccagg tggtgatggg gtcggtggac
     8461 ttgacgaggc gcatccctgc gtccgcgaac ttctgtagtg cagcgtccgc ctgcggcgtg
     8521 aagtcggctg cgaggccgcc tttgccgtcg gggacggtga cggccgacat gcagtccgtc
     8581 atcaggtaga ccttcgctgc gagcttggga tcctgcgccg cgatttcagt gagtaggtca
     8641 tcgatggtcg acttcacgca gtggctcgcg gcttgacccg cgatcacgac tgcgtcggcg
     8701 gcgagcaacg tgtggatgaa ttgcgtgttg cgctgcgcga gcgctgctcc gtcgaagcga
     8761 gacaacacct cgggtcgcag caccgagtag ttctcggtca gcgggttgcc gccctttacc
     8821 tcgacgttgc tctgcgcgac gcgcgtgaac gcatggaaga ggcgcgcttc gtggatgacg
     8881 ccggcgagcg cgtgcccatc ggaaccgagg agacagtgcg gcggccagag gtagagctgg
     8941 tacttgccgg cgcgctcgag ctcctggcag tagaacagcg cctgcttgca gagccacgtg
     9001 tagttgccac cgcacagcca cttcgccatc gccgggttcg gccgcacctc gcccgccgcg
     9061 atcatcgcgc tcgtgatcgt cgtgaacgcg gcgacgggct gatccgcctt gtcgagccag
     9121 aagctcggaa agaagatctg atacgcgagg tgcgtgtcga gcgtcgtcgt gatgtccgtg
     9181 atcgaggcga ggttgcggta gacaaactcg gcgatccgcc gcgagtcatc gatcgcgcct
     9241 ttgcccgagc gtcccgcgac gtagagcgtg ccttccggga agcagaagtc tttctgcacg
     9301 tcgatgagca agagatgcac gcgcgtctcg tcggccgcgg ccggcttgag cttgtgctgc
     9361 gctcgccacg ccgcgccggc gctcgcgagc tgcgcagcgt ccggccgata gctccagtca
     9421 gccgcgcgct tcggatcgaa gaaagtcgga atgggaagtg tcatcgaggt ctcctttcaa
     9481 ctgagttgca tccgaatggc atcgcgacgc cgaacgacgt cgagcccacc gggagaaagc
     9541 gaaagtcggt cggccgcacc gacgagcggc gcggtctccg cgaacacacg cgtgcaagca
     9601 atggcgccct gcaggatttc gatgcgcgcg acaccatcgt cggtcggcac gaacaagaag
     9661 ttgccggccg cgcacgcgcc gccaacacca gcgagccacg tcgcgtcgac gagcatctcg
     9721 cttgcgacca cgcgcgcgtt cgcatcgacg accacgcagc tggtgccctg ctccgtcgta
     9781 aagaacagcc acgcacgtgc atcggcgccg gtctgcgcgc cgccggcgat caccgcatgc
     9841 gcatcgagca agcggccgcg gagcttcggc agcgctacgc ggtcatcgag cccgccgcga
     9901 tcgggcttga acacgaagcc gaccgcatac ccacccgcgc gatagaagcc gacgcccatc
     9961 gagtcgccga cccacgcgcg cgtctgtccc gcaagcacgg agccgatgcg ctcggacccg
    10021 acgcgcgtcc gtcgcatcag cgcaccacca tcgagccaca cggcggtccc tttcatcgat
    10081 tgcatgaatg aactttcatt catcggttgc accggcacgc cgtgcgtcgc ggccggcgcc
    10141 acctctgacg tcgcgatgac ctggtaccgg aggcggccgt ggacgaccga cggcggcacg
    10201 aacgcccgcg tctgacagct cggacaccgc acgcgcgcgt gctcgtcacc gcagctcttg
    10261 catcgctgca gccgcaacct ctcgagcgcg acgcgcggat actcaccgcg cacatcgcgc
    10321 tcgaaccaat cgcggaacag cgcgagcagg tcgtcgggca gcatctgcag cggcttggct
    10381 gcacgcgggt agacgacgtc gctcgcaagc acgctcacgc gccgcaacgc acgctgcgtc
    10441 gccgggcaac tcttcgccac gccgccccac ggcccgacgc cgagcagcga gcgaaacgcc
    10501 atgaccgcga atgcgaacca gtcgctgtcc gcatcatgcg gacgaaccgg cacgagctgc
    10561 tgcgcatcgc acagccgcgg atcgacgaaa cgctcgctga acatcgagca cgcaaagccg
    10621 ccgtactgat agctatcgac gtcgatgagg tgcactcgcc gcccgtcgac gagcacgttc
    10681 aagtcgttgc agtcgccgat gacgacgccc gcgcgatgca gccccgcgat cgcatcgtgc
    10741 agcgcgacga gcgcggcaac gacgtccgca ccatcgacgg gattctcgcg acgccaccgc
    10801 ggctcgccgt acgaatgcag cggcacgccg gtgatcttcg gcatgacgta gcccacgcac
    10861 tcgccctgca cggccgccgc acccgtgatc cctcccgcga tcgcggccgc gcccaaccgc
    10921 gcgctcgcct tcttactcgc gagggcgagg cccgacggag cgatgaccgc gctcggcaga
    10981 ttgccgggca acgcgcgcag cttcgcgggc tgctcggcga gccggcgctc cgcggcagcc
    11041 tgctgctcgg gcagtccatc gaagtcggga tggtcagcgg gcttccacca cttgaggacg
    11101 cgtccatcac cgaggtcgta gacctccgct tcgccgccct gcccgatcag cgccgcaggg
    11161 tcgagcgcca cacgcgtgcc atcgatccag acgtgccgcg cagtcacgac agcacctcgc
    11221 gctcgacgcc cgtcacaccc gtcgcgctcg tcgcgatgcc cacgcgctcg atgtgtagca
    11281 ctccgatcgc gcagtcgtcc tgcaccatcg cgggcgtgcg aatcacgcga cgctcatccc
    11341 agtcgatgcg ctcttgcggc ttcgcgagca acgagagctc gcgcctcaac gcatcgggat
    11401 ggtcgagata ccgccgctgc gcgaagcgct cgatgccccc gacaatctcg cccgctccgt
    11461 cggtgccgac gatgatcgtc ccgtggtgac cgtgcccacg tgggcacatc
//
LOCUS       DATLBZ010000500         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2884397, whole genome shotgun sequence.
ACCESSION   DATLBZ010000500 DATLBZ010000000
VERSION     DATLBZ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2884397"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..658)
                     /locus_tag="VMZ53_25255"
     CDS             complement(50..658)
                     /locus_tag="VMZ53_25255"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HUS31843.1"
                     /translation="MVIRGTTRVRCPACNTEQDAQLVQTINPSENPADKEKLLAGELN
                     VLACACGKRTQLAANVLYTQDDFHCQVVPGGDDKAMEEAAAAFAASGIGGRRRLVPSL
                     NALVEKVKLLDAGLDDRAIEMAKVLLLATIGELERVLLFDGRDNQLVHWVMFDEDGQP
                     ERVSSAVDQIEKLAQRPMSDDELRIDRAWGLEAVKAMVMSAN"
     gene            complement(668..886)
                     /locus_tag="VMZ53_25260"
     CDS             complement(668..886)
                     /locus_tag="VMZ53_25260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31844.1"
                     /translation="MSLADLSGMDKAKQTPGDRVGRKRKPRDPSPEREEEVRRGWNPA
                     DDIFGEKTVDKDLDLDRGKYSTPPPRRG"
     gene            complement(896..1885)
                     /locus_tag="VMZ53_25265"
     CDS             complement(896..1885)
                     /locus_tag="VMZ53_25265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31845.1"
                     /translation="MKKLGVMLAVLLGTAPALAQPGTPTPSDPPPDPQPAQPTSPGPT
                     STLDSGIGMPSMTTTPGADKPAPPPEKKKEPGRGDFDAGGQVRLPNGPGEDDKYAAFN
                     WIAFDAKGRYFLLDQVTLNGNVPIAVIHPDTIKGGAVEPSMFGGISIRLDAIVTAPKM
                     PMVPQKGENKIGLSLTGAYMREGAMLLTEKDFPLFIGDMKPGFAGALVTRVALSNVVD
                     FSLLPAWIYQSGSTNSHKAIQVPLALIIKLGDVVKLSTDLNIATGDDYSMRGKNGGRL
                     GAGVALDLKIGPILGHVGTGVASLLTGDPMTTQAVYPSIKDSVYFDLNVKYAK"
BASE COUNT          334 a          666 c          658 g          403 t
ORIGIN      
        1 catgtcgaga tcgggctcgg cgatccgaac gcgatcgaca aggcgctcgc tagtttgcgc
       61 tcatcaccat cgccttgacg gcttcgaggc cccacgcgcg atcgatgcgg agctcgtcat
      121 ccgacatcgg ccgctgcgcg agcttctcga tctggtcgac ggcgctcgag acgcgctcgg
      181 gctgaccgtc ctcgtcgaac atcacccagt gcacgagctg gttatcgcgt ccgtcgaaca
      241 ggagcacgcg ctcgagctcg ccaatcgtcg cgagcagcag caccttcgcc atctcgatcg
      301 cccgatcgtc gagccccgcg tcgagtagct tcactttctc gacgagcgcg ttgagcgaag
      361 gcacgagccg gcgcctgccg ccgatgcccg atgctgcgaa cgccgcggcc gcttcttcca
      421 tcgccttgtc gtcgccgccc ggcacgactt ggcagtgaaa gtcatcctgc gtgtagagga
      481 cgtttgcggc gagctgtgtc cgcttgccac atgcgcacgc gagcacgttg agctctcccg
      541 cgagcagctt ctctttgtct gcgggattct cgctggggtt gatcgtctga acgagctgcg
      601 cgtcttgctc ggtgttgcat gccgggcagc gcacgcgcgt cgttccgcga atcaccatct
      661 cgcaggttta gccgcgacgg ggcggcggcg tcgagtactt gccgcgatcg agatcgagat
      721 ccttgtcgac ggtcttctcg ccgaagatgt catcggctgg gttccacccg cgacggacct
      781 cttcttcacg ctcgggagaa gggtcacgtg gcttgcgctt gcgacctacg cgatctcccg
      841 gtgtctgctt cgccttgtcc ataccggaca agtcagcaag cgacatgccg cgacgctact
      901 tcgcgtactt gacgttgagg tcgaagtaga cggagtcctt gatcgacgga tacaccgcct
      961 gcgtcgtcat cggatcgccg gtgagaaggc tcgcgacgcc cgtgccgacg tggccgagga
     1021 tcggaccgat cttgagatcg agtgcgacgc ccgcaccgag gcgaccgccg ttcttgccgc
     1081 gcatcgagta gtcatcgccg gtcgcgatgt tcagatcggt gctgagcttg acgacgtcgc
     1141 cgagcttgat gatcagcgcg agcggaactt ggatcgcctt atgcgaattc gtgctgcccg
     1201 attgatagat ccacgccggc agcagcgaga agtcgacgac gttcgacagt gcgacgcgcg
     1261 tgacgagcgc acccgcgaag cccggcttca tgtcgccgat gaacagcggg aaatctttct
     1321 cggtgagcag catcgcgcct tcgcgcatgt atgcacccgt tagcgacaac ccgatcttgt
     1381 tctcaccctt ctgcgggacc atcggcatct tcggcgcggt gacgatcgcg tcgaggcgaa
     1441 tcgagatacc gccgaacatg ctcggctcga cggcgccacc cttgatcgta tcgggatgga
     1501 tcaccgcgat cgggacgttg ccgttcagcg tcacctgatc gagcaggaag tagcggccct
     1561 tcgcgtcgaa cgcgatccag ttgaaggcgg cgtacttgtc atcttcgccg gggccgttcg
     1621 gcaggcgcac ttggccaccc gcatcgaagt cgccgcgacc gggttccttc ttcttctccg
     1681 gcggcggcgc tggcttgtct gcgccgggcg tcgtcgtcat cgacggcatg ccgatgcccg
     1741 aatcgagcgt cgacgtcggc ccaggcgacg tgggttgtgc cggctgcgga tcaggcggcg
     1801 gatcagacgg ggttggcgtg ccgggttgcg cgagcgccgg tgctgtcccc aacagcaccg
     1861 ccagcatcac accgagtttt ttcattgtta gctgccagta gacggcggag gtccgtgaga
     1921 cctccagtcc cccaaccgtt aggcagcagt gattctactc aagtgggcga tcgctgcgcc
     1981 agatttgcac ctgcggcgag ccgcgcgcga tgtaggcgta gcccagatcg tcgaatgcga
     2041 cggagcgcag ctgcgacgcg g
//
LOCUS       DATLBZ010000501         7965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2891010, whole genome shotgun sequence.
ACCESSION   DATLBZ010000501 DATLBZ010000000
VERSION     DATLBZ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2891010"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VMZ53_25270"
     CDS             <1..967
                     /locus_tag="VMZ53_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS31846.1"
                     /translation="AGLDNVSYAIAMEEVSRACASTGVIMSVNNSLVCDPIYRFGTEA
                     QKTEWLTPLASGKLLGCFALSEPEAGSDAAAQKTVAEKQSDGTWVINGTKNWITNGPV
                     ADVCVLFTMNDKALGHKGITAFILPMKTKGVRTGPPDDKLGIRGSKSSQIYLDEVRLP
                     ADAILGEVGGGFRVAMSTLDGGRIGIAAQALGIARAALEDALAYAQQRRTMGKPIIQH
                     QAISFKLADMATEVDAARLLVHRAAWLKDNKLPYGKEAAMAKLFASDIANKAAREAIQ
                     IFGGNGYVTEFPVERHFRDAKITEIYEGTSEIQRLVISGYLAKGG"
     gene            970..1410
                     /locus_tag="VMZ53_25275"
     CDS             970..1410
                     /locus_tag="VMZ53_25275"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS31847.1"
                     /translation="MKNTILVAVLVIAACGGKNKNNAEEGGGATIDTQATSGDTTDHS
                     GSMVPPEKMEEVNSALLRKNNIISRCLSQAVEAGTAPKGTHGKITVELSISPSGQATK
                     VEVIKSSIESKEVQGCVKRKVEEISFPELPKQYETSYTYAMEAN"
     gene            1410..2228
                     /locus_tag="VMZ53_25280"
     CDS             1410..2228
                     /locus_tag="VMZ53_25280"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS31848.1"
                     /translation="MRLAVPALFLAACGSSSPPPAKPDPVVEKKVTRVPIEDESKDEV
                     PEEGVTFTKTKGAMAKEAIEAGLAPHTQELSDCYIKKVGKRRWLGGHIVLHWDIKADG
                     TITSVKMAESDLGAWDIEKCLVEIAWAATFEKPAGGDADFTVPLEFSPTRSSQVWDED
                     KALRAVGGQLANLDECDHPDNDEKTKGGHKKKAPPKKDPDAKPVRPEPRPPSNVIVTL
                     YVGPQGKAQSVGFSSPTSELGEKWTACAEQAAMNWRLPDPRGQIAKLAVRYRAE"
     gene            2232..3389
                     /locus_tag="VMZ53_25285"
     CDS             2232..3389
                     /locus_tag="VMZ53_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS31849.1"
                     /translation="MLFSPTEEQELVQQTARDFAERVLAPKAAARDLSGEFPEKELRE
                     LGKLGLLGVNVPDALGGADAGVIAYSLALQELARGDASVAVAVSVTNMVAELIATQGS
                     AELAQQWVPRLVSGELVAGAFGLSEPEAGSDAGAMRTTATKTANGWRLSGTKQWITSG
                     DHAGVIVVWATTGDGGAQSAKHAGMSAFIVPGDAKGLSVARLEEKMGLHGSSTAQLVL
                     DGVEVGDDQILGAVGRGFSLAMMALDGGRIGIASQACGIARGALEAAVTYAKDRQTFG
                     QPIIKHQAVGNMLADAATWLDAATLMTLRAAWTKQEKQPFSQAAAMAKLFATEHAWKI
                     CDIALQVHGGYGYVRDFPVERALRDVRVTRIYEGTSEIQRIVIARNLMRSQ"
     gene            3393..3800
                     /locus_tag="VMZ53_25290"
     CDS             3393..3800
                     /locus_tag="VMZ53_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HUS31850.1"
                     /translation="MSDRKLRILVAKPGLDGHDRGAKVVARALADAGYEVVYTGLHQT
                     PEMIAAAAVQEAVDAVGLSIMSGAHMTLFPAVIDALKSRGAQDVIVFGGGIVPKDDLP
                     KLTEMGVAKIFTPGASTNEIVDWVENFLRPKVA"
     gene            3800..4960
                     /locus_tag="VMZ53_25295"
     CDS             3800..4960
                     /locus_tag="VMZ53_25295"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUS31851.1"
                     /translation="MRAALAAALFGVVLSACSQPAPPATKPAGSGTGSGLSMVPDDAG
                     PATQEETLAAIQKAMNELDEGSQTCWAVAATDRFDIEGDLVMRIDVGPTKATAAVVTD
                     NVHNTKLTACMSELLGAYRWAPPLYGQTIQLPFKFRHPDGQSVIDRRLVEAHGQTNIA
                     VSVLLDEANTGNGAASMFEINLAEGASTDWRKADRAELWYFLTDAIVANSPKQMHVYS
                     PVKAGDMVYVAAGKSRKFSGRVGGTRAVLVVAPGGREGTARAGALPTPAAPAGGTPEI
                     LYATGAKTWGPAKIYLDSAELTDAPFAGSILAMPAGAKVAEHVHEKETEMLYLLEGSG
                     AMTIAGQRVEVTETSVIQIPPNTKHSFEASTAVRAVQVYTPGGPEQRFKKKP"
     gene            4957..5898
                     /gene="meaB"
                     /locus_tag="VMZ53_25300"
     CDS             4957..5898
                     /gene="meaB"
                     /locus_tag="VMZ53_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HUS31852.1"
                     /translation="MSSIDSILQSDIRAAARLMRDLDDRRPDAMTMLKQLFPHTGKAY
                     IVGITGNPGAGKSTVVDSLIAHYRSKGERVGVVCVDPTSPFSGGAILGDRIRMQRHAL
                     DPDVFIRSLATRGHLGGLSRSTFDVAHVLDAMGYQRILIETVGVGQDEVEVMRAAHTT
                     VVVTVPGLGDDIQAIKAGLLEVADVLVVNKADREGADRTERDLMHMLDLRTGDRKDVE
                     IVRTIATRGTAEGSGIAELADGIEKHRERAWGGEGANARAVLRATAQLAELVRAQLAD
                     RASKALAQRGGLSEIAQAVVDRRQDPWTIAEELVETL"
     gene            5993..6688
                     /locus_tag="VMZ53_25305"
     CDS             5993..6688
                     /locus_tag="VMZ53_25305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31853.1"
                     /translation="MTSFRYCLGALLLSGCGLISSDVTNFDLTLPEKAFSVDASGWQV
                     NQEAADAFLGTSCAGSPNICSSAATNACPMNCTGECSTTTSKCVLHLDVGVYQMIDLV
                     SEKPELKSINDEPVIKVTIDSVTYRVPQNTLNTDTPQMNVYVAPMSVMDPKDPMAKQI
                     GTIAPVAAGATVGMADMAYTANGKQNLMDVMSTYKNPFNVIVGSDLEIQQGDPVPQGR
                     LDAVVQIKAHAGL"
     gene            6708..7238
                     /locus_tag="VMZ53_25310"
     CDS             6708..7238
                     /locus_tag="VMZ53_25310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31854.1"
                     /translation="MVRAVLLTTLLAGCLSVGSDPPGPGSNNNPDGGTSGNVDAPKLV
                     WPDAAPGMMNNLPCKNTVTPAPQLGHHNTGQSCMQSCHNHGFTVAGTLYTNDTGNTGF
                     AGAHVTLTDNNGTAHDLVVNSDGNFYTKTTVAFPVLVIASACPSAVKMPLASSDGNCN
                     KTGCHVGGGPRQMHLP"
     gene            7400..>7965
                     /locus_tag="VMZ53_25315"
     CDS             7400..>7965
                     /locus_tag="VMZ53_25315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31855.1"
                     /translation="MKIQFRSILFALVLPVAACGGGEDTNMVSMAPQDFLPKPVVSQP
                     VVETPVVTKTPIVEIPVQEPVAKAETEIKAKPGTAQYHIEMARMYISQDKRALAIASA
                     NKAIKLAPNSSQAWNTKGRAELNAKNYENAITAFTTAVEMNPDNAFAWNNLGYTELLL
                     KRYEDAAEHLGEATTKKDATGYMFNNLGT"
BASE COUNT         1579 a         2501 c         2639 g         1246 t
ORIGIN      
        1 cgccggcctc gacaacgtca gctacgcgat cgcgatggaa gaagtctcgc gcgcgtgtgc
       61 gtcgacgggc gtcatcatgt ccgtcaacaa ctcgctcgtg tgcgatccga tctatcggtt
      121 cggcaccgag gcgcagaaga cggagtggct cacgccgctc gcgagcggaa agctgctcgg
      181 ttgcttcgcg ctcagcgagc ccgaagcggg cagcgatgcc gcggcgcaga agaccgtcgc
      241 cgagaagcag tccgacggca cgtgggtcat caacggcacg aagaactgga tcacgaacgg
      301 ccctgtcgcc gacgtctgcg tgctgttcac gatgaatgac aaggcgctcg gccataaggg
      361 catcaccgcg ttcatcctgc cgatgaagac gaagggtgtc cgcacgggcc cacccgacga
      421 caagctcggt attcgtggca gcaagtcatc gcagatctac ctcgacgagg tgcgcctccc
      481 cgcggatgca atcctcggcg aagtcggcgg aggcttccgc gttgcgatgt ccacgctcga
      541 cggcggccgc atcggcatcg ccgcgcaggc actcggcatc gcacgcgcag cactcgaaga
      601 tgcgctcgcc tatgcgcagc agcgccgcac gatgggcaag ccgatcatcc agcaccaagc
      661 gatcagcttc aagctcgccg acatggcgac cgaggtcgat gccgcgcgct tgctcgttca
      721 ccgcgccgcg tggctaaagg acaacaagct tccgtacggc aaggaagccg cgatggcgaa
      781 gctgttcgcc tcggacatcg cgaacaaggc cgcgcgcgaa gcgatccaga tcttcggcgg
      841 caatggctac gtgacggagt tcccagtgga acgtcacttc cgcgatgcca agatcacaga
      901 aatctatgaa ggtacgagcg agatccagcg tctcgtgatc agcgggtact tagcaaaggg
      961 tggatgacta tgaagaacac gattctcgtt gcagtactgg tgatcgcggc atgcggtggc
     1021 aagaacaaga acaacgctga agagggcggc ggcgcgacga tcgacacgca agcgacgagc
     1081 ggcgatacga ccgatcactc gggcagcatg gttccgccgg agaagatgga agaggtcaac
     1141 tcggcgctgc ttcgcaagaa caacatcatc tcgcgctgcc tatcgcaggc ggtcgaggcg
     1201 ggcaccgcgc cgaagggcac gcacggcaag attaccgtcg agctttcgat ctcgccttcg
     1261 ggtcaggcga cgaaggtcga agtgatcaag tcgtcgatcg agtccaaaga agtgcagggc
     1321 tgcgtgaagc gcaaagtcga ggagatttcg ttccccgagc tgccgaagca gtacgagacc
     1381 tcgtacacgt acgcgatgga agcgaactga tgcgattggc agtacccgca ttgttccttg
     1441 cggcatgcgg ttcgtcatcg cctcctcccg cgaagcccga tccggtcgtc gagaagaagg
     1501 tcacgcgcgt tcccatcgag gacgagtcga aggacgaggt ccccgaggag ggcgtcacct
     1561 tcacgaagac gaagggcgcg atggcgaagg aagcgatcga ggcaggtctt gcgccacaca
     1621 cgcaagagct ctctgattgc tacatcaaga aggtcggcaa gcggcgctgg ctcggcggcc
     1681 atatcgtcct gcattgggac atcaaggccg acggaacgat cacgtcggtg aagatggccg
     1741 agagcgacct cggcgcgtgg gatatcgaga agtgtctcgt cgagatcgcg tgggcggcga
     1801 cgttcgagaa gccggccggc ggcgatgcgg acttcacggt cccgctcgag ttctcgccga
     1861 cgcgcagctc gcaagtctgg gacgaggaca aggcgctgcg cgccgtcggt ggccagctcg
     1921 cgaacctcga cgagtgcgat caccccgaca acgacgagaa gacgaaaggc ggccacaaga
     1981 agaaggcgcc gcccaaaaaa gatccagatg caaagccggt gcgtcccgag ccacgtcctc
     2041 cgagcaacgt gatcgtcacg ttgtatgtcg gcccgcaagg caaggcgcaa tcggtgggct
     2101 tctcgtcgcc gacgagtgag ctcggggaaa agtggacggc gtgtgcggaa caggcggcga
     2161 tgaattggag gctccctgat ccgcgcggcc agatcgcaaa gctggcggtg cggtacaggg
     2221 cggagtagcc cgtgctgttc tcgcctaccg aagagcaaga gctcgttcag cagaccgccc
     2281 gtgacttcgc ggagcgtgtg cttgcaccaa aggcagccgc gcgcgaccta agcggagagt
     2341 ttcccgagaa ggagctgcgc gagctcggca agctcgggct cctcggtgtc aacgtgccgg
     2401 acgcgctcgg cggagcagac gctggcgtca tcgcgtactc gctcgcgctg caggagctcg
     2461 cgcgtggtga tgcatcggtg gccgtcgcgg tctcggtcac gaacatggtc gccgagctga
     2521 tcgcgacgca aggcagcgca gagctcgcgc aacagtgggt gcctcggctc gtaagtggcg
     2581 agctcgtcgc cggcgcgttc ggcctctcgg agcccgaggc ggggagcgat gcgggcgcga
     2641 tgcggacaac cgcgacgaag accgcgaatg gctggcggct ctccggcacg aagcagtgga
     2701 tcacgagcgg tgatcacgcg ggcgtgatcg tcgtgtgggc gaccaccggt gatggcggcg
     2761 cgcagtccgc gaagcacgca ggcatgtccg cgttcatcgt gcccggcgac gcgaagggac
     2821 tgtcggtcgc gcgcctcgaa gagaagatgg gcctgcatgg ctcgtcgact gcgcagctcg
     2881 tgctcgacgg tgtcgaggtc ggtgacgacc agatcctggg cgcggtcggt cgcggcttct
     2941 cgctcgcgat gatggcgctc gacggcgggc gcatcggcat cgcctcgcag gcatgcggca
     3001 tcgcgcgcgg cgcgctcgag gcggccgtca cctacgcgaa ggatcggcag acgttcggcc
     3061 aaccgatcat caagcaccag gcggtcggaa acatgctagc cgatgcagcc acctggctcg
     3121 acgccgcgac gctgatgacg ttgcgcgcgg catggacgaa gcaggaaaag cagccgtttt
     3181 cacaggcggc ggccatggcc aagctgtttg cgaccgagca cgcctggaaa atctgcgaca
     3241 tcgctctcca agtgcacggc ggctacggct acgttcgcga cttcccagtc gagcgtgcgc
     3301 tccgcgacgt tcgcgttacc cgcatctatg aaggtacgag cgagatccag cgcatcgtca
     3361 tcgcgcgcaa tctgatgagg agccagtgag tcatgagtga tcgaaagcta cgcatcctgg
     3421 ttgccaagcc cggtctcgac ggacacgacc gcggcgcgaa ggtcgttgca cgcgcgctcg
     3481 cggatgccgg ttacgaggtc gtgtatacgg ggctgcatca gacgcccgag atgatcgcgg
     3541 ctgccgccgt gcaggaagcc gtcgacgcgg tcggcctctc gatcatgagc ggcgcgcaca
     3601 tgacgctctt cccggccgtg atcgacgcgc tgaagtcgcg cggtgcgcaa gacgtcatcg
     3661 tgttcggtgg cggcatcgtg ccgaaggacg acctgcccaa gctcaccgag atgggcgtcg
     3721 cgaagatctt cacgcccggc gcgagcacga acgagatcgt cgactgggtc gagaacttcc
     3781 tgcggcccaa ggtcgcctga tgcgtgcagc gctcgcagca gcgctgttcg gcgttgtgct
     3841 gagcgcgtgc agtcaaccag cgccgcctgc gacgaagccg gcaggcagcg gcaccggctc
     3901 gggcctctcg atggtcccgg acgatgcggg tcccgcgacg caagaggaga cgctcgccgc
     3961 gatccagaaa gcgatgaacg agctcgatga gggctcgcag acatgttggg cggtcgcggc
     4021 gaccgatcgc ttcgacatcg agggtgacct cgtgatgcgc atcgacgtcg gcccgacgaa
     4081 ggcgacggca gcggtcgtca ccgacaacgt gcacaacaca aagctcaccg catgcatgag
     4141 cgagctgctc ggcgcgtatc gctgggcgcc gccactatac gggcagacga tccagctgcc
     4201 gttcaagttc cggcatcccg acggtcagtc ggtcatcgac cgccgcctcg tcgaagcgca
     4261 cggccagaca aacatcgcgg tgtccgtgct gctcgatgaa gcgaacaccg gcaacggcgc
     4321 agcctcgatg ttcgagatca atttggcgga gggcgcgtcg accgactggc gcaaggccga
     4381 ccgcgcggag ctctggtact tcctgacgga tgcgatcgtc gcgaactcgc caaagcagat
     4441 gcatgtctac tccccggtga aagccggcga catggtctac gtcgccgcgg gaaagagccg
     4501 caaattcagc ggccgcgtcg gcgggacacg tgccgtgctc gtcgtcgcac caggcggtcg
     4561 cgaaggcacc gcgcgtgcgg gtgcgctccc gacgccggct gcgcctgcag gcggaacgcc
     4621 ggagattctc tacgcgaccg gcgcgaagac atggggaccg gcgaagatct atctcgacag
     4681 cgcggagctc acggacgcac cgttcgccgg atcgatcctc gcgatgcctg ccggcgcgaa
     4741 ggtcgccgag cacgtgcacg agaaagagac ggagatgctg tacctgctcg aaggcagcgg
     4801 cgcgatgacg atcgccggcc agcgcgtcga ggtcaccgag acgtccgtca tccagattcc
     4861 tccgaacacg aaacattcgt tcgaagcgag cacggcggtg cgcgcggtcc aggtctacac
     4921 gccgggcggt cccgagcagc gattcaagaa gaagccatga gcagcatcga ctcgattttg
     4981 caaagtgaca tccgcgcggc cgctcgcttg atgcgcgacc tcgacgatcg tcgtcccgat
     5041 gcgatgacca tgctgaagca gctgttcccg cataccggca aggcatacat cgtcgggatc
     5101 accggcaacc caggcgccgg aaaatcgacc gtcgtcgact cgctcatcgc gcactaccgc
     5161 agcaagggcg aacgcgtcgg tgtcgtctgc gtcgatccga catcgccgtt ctccggcggc
     5221 gcgatcctcg gcgatcgcat tcgcatgcag cgccatgcgc tcgatcccga tgtcttcatt
     5281 cgcagcctcg cgacgcgcgg tcacctcggc ggcctgtcgc gctcgacgtt cgatgtggcg
     5341 cacgtgctcg acgcgatggg ttaccagcgc atcttgatcg agacggtcgg cgtcggtcag
     5401 gacgaagtcg aagtcatgcg cgccgcgcac acgacggtcg tcgtgaccgt gccgggcctc
     5461 ggcgatgaca ttcaagcgat caaggcgggc ctgctcgaag tcgccgacgt cctcgtcgta
     5521 aacaaggccg atcgcgaggg cgccgaccgc acggagcgtg acctcatgca catgctcgat
     5581 ctccgtacgg gcgatcgaaa agacgtcgag atcgtgagga cgatcgcgac acgtggcaca
     5641 gcggaaggtt ctggcattgc cgagctcgcc gacggaatcg agaagcatcg cgagcgcgcg
     5701 tggggaggcg agggcgccaa tgcgcgcgcc gttctacgcg ccaccgcgca gctggcagag
     5761 ctcgtccgcg cgcagctcgc agaccgggcc tccaaagcgc tggcgcagcg tggggggctc
     5821 tctgaaatcg cgcaggctgt cgtcgatcgc cgtcaggatc cgtggacgat tgccgaagag
     5881 ctcgtagaga cgctgtaggt ccctgtagcg tccatcacgg cgggtccaga atttgggtcc
     5941 gctcgcagca cgggtgctag actgagcttg ccccggggtt ttaggggcac ttatgactag
     6001 ttttcgctat tgcctcggtg ccctcctgtt gtccggctgc ggcctgatct cgtcggacgt
     6061 aacgaacttc gatctgacgc tgcccgagaa ggcattcagc gtagatgcgt ctggctggca
     6121 ggtgaaccag gaagctgccg acgcgttcct cgggacgagc tgcgcaggct cgccaaacat
     6181 ctgctcctcg gccgcgacga atgcgtgccc gatgaactgc acgggcgagt gcagcacgac
     6241 gacgagcaag tgcgtgctcc atctcgacgt cggcgtgtat cagatgatcg acctcgtctc
     6301 ggagaagccg gagctcaagt cgatcaacga cgagcccgtc atcaaagtga cgatcgacag
     6361 cgtcacgtat cgcgttccgc agaacacgct gaacacggac acgccgcaga tgaacgtcta
     6421 cgtcgcgccg atgtcggtga tggatccgaa ggatccgatg gcgaagcaga tcggcacgat
     6481 cgcgccggtc gctgcaggtg ccaccgtcgg catggcggac atggcctaca ccgccaacgg
     6541 caagcagaac ctcatggacg tcatgtcgac gtacaagaac ccgttcaacg tgatcgtcgg
     6601 ctcggatctc gagatccagc aaggcgatcc ggttccgcag ggccggctcg acgcggtcgt
     6661 tcagatcaag gcccacgcgg gcctgtagcc gcgataccat cgccgtgatg gttcgcgctg
     6721 ttctgctgac cacgctgctc gctggttgtc tgagcgtcgg ctcggatcca ccgggcccgg
     6781 gcagcaacaa caatcccgac ggcggcacgt ccggaaacgt cgacgcgccg aagctcgtgt
     6841 ggcccgacgc ggcgcccggc atgatgaaca atctgccgtg caagaacacg gtcacgcccg
     6901 caccgcagct cggccatcac aacaccggcc agagctgcat gcagagctgt cacaaccacg
     6961 gcttcacggt cgcgggcacg ctctacacga acgacacggg caacaccggg tttgccggcg
     7021 cgcacgtcac gctcaccgac aacaacggca ccgcgcacga cctcgtcgtg aattctgacg
     7081 gcaacttcta cacgaagacg acggtcgcgt ttccggtgct cgtgatcgcg agcgcatgtc
     7141 catccgccgt gaagatgccg ctcgcgtcgt cagacggtaa ctgcaacaag acgggctgcc
     7201 acgtcggcgg cggtcctcgt cagatgcact tgccgtagat ctcgtctaaa acgccgcaga
     7261 atcgcattct gttgcatgcg tgccggcgag gacgctggcg tgtcccggca cccacaggtg
     7321 cccacaagtt ggcgtgcgcg ttcgaagtct cgtctggcac gtcgggtgaa gaaaggagag
     7381 agcgaaaggg atacgtgtca tgaagatcca gtttcgttcg atcctgtttg ctctcgttct
     7441 tccggtggcg gcgtgcggtg gcggcgagga caccaacatg gtgtcgatgg cgccgcagga
     7501 tttcctgccg aagccggtcg tgtcgcaacc tgtcgtcgag acgccggtcg tcacgaagac
     7561 gccgatcgtc gagatcccgg tgcaagagcc cgtcgcgaag gcggagacgg agatcaaggc
     7621 caagccgggc acggcgcagt accacatcga gatggcgcgc atgtacatct cgcaagacaa
     7681 gcgcgcgctc gcgatcgcgt cggcgaacaa ggcgatcaag ctcgcgccca attcgagcca
     7741 agcgtggaac acgaagggcc gcgcggagct caacgccaag aactacgaga acgcgatcac
     7801 ggcgtttacg actgcggtcg agatgaaccc ggacaacgcg tttgcgtgga acaacctcgg
     7861 ctacaccgag ctgctcctca agcgctacga agacgccgcc gagcatctcg gcgaagcgac
     7921 cacgaagaag gacgcgaccg gctacatgtt caacaacctc ggcac
//
LOCUS       DATLBZ010000502        17765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8526029, whole genome shotgun sequence.
ACCESSION   DATLBZ010000502 DATLBZ010000000
VERSION     DATLBZ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17765
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8526029"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VMZ53_25320"
     CDS             complement(<1..275)
                     /locus_tag="VMZ53_25320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31856.1"
                     /translation="MTALFTAGALAKAWLATLLMMAAQGTLLALLAFVLARAGKLRPA
                     WQAAIWLVVTVKLALPWGPSMPYSLADAIAMFRDHPAPAPMLHIAPSA"
     gene            complement(272..697)
                     /locus_tag="VMZ53_25325"
     CDS             complement(272..697)
                     /locus_tag="VMZ53_25325"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HUS31857.1"
                     /translation="MKCPELTPAELRVMKALWELGKGSVADVRGELDKRGNELAYTTV
                     MTLLGRLATKNAVVVDKAREPFVYRPAFRRESMLRDRLRDFVREVFDGKADSLVLRLV
                     EDESLSREDLIEIERKIADAEKPAAEKPEKPEKPKKERK"
     gene            complement(694..1533)
                     /locus_tag="VMZ53_25330"
     CDS             complement(694..1533)
                     /locus_tag="VMZ53_25330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31858.1"
                     /translation="MNWWFAPAPEGRLRALRILIGGYALVYLVARMPEIYKVAHYGRE
                     HFAQVGVTRLLDAPLAPALVLAIAIATCALLACLVANVQYRITAPLAALGLLWTITYR
                     NSWGMVFHTENLLVLHVIALALSPPKGDAGWPVKLMATLTTLTYLLAGIAKLRIAGPA
                     WIDGELLRNQIAVDNLRKALLGDPTAPLANLFLHHPKQLVVFSAMTLVIELGAPLALL
                     HRRIGYVWAVTAWAFHLGVVLLMNIWFPYPLLGFAFLPLLPAERLIARATTLVVMLRK
                     RSS"
     gene            complement(1530..1973)
                     /locus_tag="VMZ53_25335"
     CDS             complement(1530..1973)
                     /locus_tag="VMZ53_25335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31859.1"
                     /translation="MDRARWLGLAVSLALVAATLEPLVRDPYDDGFPLSTYPMFATKR
                     QTVQTFHYALGVTRDGKRRTLSPSLIGSSEILQALRVIEHAVGGGKGALTSLCETIAK
                     RVASDDDFTDIAFVRIVKGTHDAVRYLGDDEIGVESELLRCEVRR"
     gene            1996..3756
                     /locus_tag="VMZ53_25340"
     CDS             1996..3756
                     /locus_tag="VMZ53_25340"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS31860.1"
                     /translation="MREVRSWVVIVVALAACKGQGGTAAKGSGEIAAEGTRLEGEIPV
                     VFGDCVPNTDTPFVSGPEPQAFTLDELKHAGNTLYVTMADGGDVHAAFDGWGTIGTGK
                     YGTVSHGSGAGASYGIGSGRLIGQRGNGPTVTIGQPVAGGGLDKAIMRRYIKRNIQKI
                     QYCYEKQLLATPAIAGTLTAKFTIIENGTVSKSEANGVHKEVASCVASVIKSIEFPKP
                     KGGNVEVSYPFTFRPGGGTTAAIKPVDSTARQQAIDAARNQGVIGALGDSGGAFATLS
                     TPRPKSKPYQPGAGSALVELRILLVDCFRNQDKNYAAVVFDLAPGAITAHGIDHPAFA
                     KCIAGIAEQVKVGGPLRCSATFGTAGVESLPAVDITADAITMFGKKVTELAAVVGGHP
                     EPWWKIESVSIAASEHVKATRQSKAPLTLHGPLAIRPLATTPMKVVTNVVHTLIDAGE
                     DPLLTAQRNGTWQLLRSYALPVVPVPRGTGGSWDSEANIFADDQDAVHLSVLVDKTDV
                     WVGISRTNEGKRVPLAKLKEELAAHKASTRFAEREDIEIAGADEVTYGQVVEAIDVAS
                     AAGFRGWSLTSPVGLSAPAR"
     gene            complement(3757..5154)
                     /locus_tag="VMZ53_25345"
     CDS             complement(3757..5154)
                     /locus_tag="VMZ53_25345"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HUS31861.1"
                     /translation="MWSSAVAALAVHAILLGSVEGFDLNVIGEGFSTRDRGTAKELVE
                     LQANCVGDALLASSARYTMCFAPWREDTDACLTGAELEMRMDLSNCEARRTPDAIAAI
                     AMLEQKNADKITPIDPEPLLEEMKQEQDKPKPPEIQQPQPQQPTPPTPPAPPPPKRPQ
                     QIVETAKPNSEKEPENARYLSEFNTVAEKQTVARGSVKEPMVAKSQPEELTPKQNPKE
                     ASIEKQDEDRAKGANKTAPDVPGSLAMRTPGVQTPSELEQEQKERGTATGGMGPLAFD
                     GFMPRKGQGAFEQQQKQRSEIPKGQSGAGGGVPDLQLKPSQEQLERALGGGNVDHMED
                     VLAGDETAVNSKRWVHATFFNRLKRQVAQNWDPASVWRRNDPSGQVYGFKTRVTEVRV
                     ALATNGQLAKIVVTAPSGVSELDDEAVRAFQSAAPFPNPPKELANGDGLITFAFSFYF
                     EIGQPRSSWKVVRSM"
     gene            complement(5295..6203)
                     /locus_tag="VMZ53_25350"
     CDS             complement(5295..6203)
                     /locus_tag="VMZ53_25350"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS31862.1"
                     /translation="MKLAALVALAVTLALPTLARADDVTIGLYAPTAPFEGSGDRVSF
                     INGIADHLKGVANGGKVTGKVYANASAFAAAVKKGEIQYAVIDAPYAAANGLPYQILA
                     TATRNNESSAPWQLVAKSSIGSIKDLKGAKVAIPTVGAKQQAFVTNVLLGAEVDAAYF
                     GSIVDAADARSAMTMITVGKADAAFVSSGVEPPAGTKRLLGLTNVGWPMFVALPGADA
                     KLTAAFSARIKSFGGAGGFSGFTDAVSYRSLAGSFSSNTKRGPMAVPPPARLNVRDLL
                     TGRTFNVPLSDPLEVVVAPAAPAPAQ"
     gene            complement(6200..8575)
                     /locus_tag="VMZ53_25355"
     CDS             complement(6200..8575)
                     /locus_tag="VMZ53_25355"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31863.1"
                     /translation="MKRIVLAALLAFATTTPAFAQREQDEESQLLVEEAKRALSKKDY
                     ERAGGLLDKALVSSPRRIDLYILRASIYGIVGKHDAAVTLLERARQLSPNNTRVNISL
                     GVELVEVGRGSEGVPILEKIVAGDARHFDAQVVLGHYYAKQGEWKDAAKAFDAYFANR
                     PKALAGEDSLHRYDQANSMLRSGDAKGALDLYKKLGEDKKDDRARLGVAWATAAISCK
                     QAMPILDNLGELEAKYAEISLVRGRCALKLGRLDDAVNRAERFRKSSPNALAGWILLG
                     DVRAAQNNWKEAEAAYLHVVEASPSDRLFALELARAERHLGKTAAAADRLKAAGPPKD
                     YEDDWTLEYGETLLVLRRAAALKELVAPFVAAHEKNGKAQFLLGSALYILGDHAQAVG
                     HLDAALDQGEQRAAPTFVDALNTLAAEAVKKKDLAQAASFLDKAAAAGGSTLTTRNLG
                     AVLVAKNDLPRALEVLQKADANDADAQYVLGRALQAAKRFDDARNAYGRAIKLYGKDA
                     RVAVVQRDLAASELAAGRGEEAVAVLDAAVQTAPAGTKKELDRARLAVARSAATEAMR
                     AARFSVAVKILRSVEKSADGDALVDLRCDLALAATGAQQRDLALDLLRGLERGKAKCP
                     FVAPADDVGVPILIAWNEDASPKRARKTLDRLETMRRRATGVAEPLLRTAATDIAVRA
                     AAEAYTAGNTKLAAQFLATAKSYDKRSPEVVHDLAVIDLVGGQTDNAMNVLANLVGEV
                     PEARINIGVAYERKGEPLKALQSWKAAAAAGSRFAPLKDWIEAKERFWGAP"
     gene            complement(8572..10209)
                     /locus_tag="VMZ53_25360"
     CDS             complement(8572..10209)
                     /locus_tag="VMZ53_25360"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HUS31864.1"
                     /translation="MRRRLSLGTKLTLGTALLIATAVASSAWYGLNSLDSYARASGEA
                     RRNDLEKAFQREAELLTRNASATSASLLATSDYTRLEEIARNLAVENRNVVWIALVEP
                     SGTVAAASDKAPAKQGGKIDDPLAKTLETAASGKVEQIPDPADKSHLLLGTPVFIPDS
                     TGKEVRVGTVRLAFDMSAMERAQQEALAAGRERARASAERQLLFAGLLLAIGLLLALW
                     QALRISRPLLALSEQARHIAAGDFGRRVVVKTGDEVGQLGESFNRMSESLVYLVEEIG
                     RKASLERELEVARSIQGLMTPPPEAVALDSFNLIGRCEMASACGGDWWSYRQLADGRL
                     LLVVGDVTGHGMPSAMIAATGRGAVESLAFINSNAITPELVLRAIDKAIRDVGERLLM
                     TCFAMILRQDGVVDYANAGHTFPYVISDTKKQPMLEILPTRSNPLGSYRPHIAEGKCQ
                     LANGDFIVLTSDGLTDRVSREGTRFGDKRLRKALMEEVTRGTNNVRALCDRVVGEVNR
                     FGGEQPVDDDITLVIVQYSGTATGSRKGINAGRGAAA"
     gene            10343..12436
                     /locus_tag="VMZ53_25365"
     CDS             10343..12436
                     /locus_tag="VMZ53_25365"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HUS31865.1"
                     /translation="MAGADDEPAIPIRELPPFPDVEASAQVSEVGVYGASQSEEETVV
                     GAAKREQSLGTVASAVTVLTADQMRRFGYRSLAEALRGISGVYVVDDRMVERIGVRGL
                     QLLGDSNTRILILIDGTPLNEPWAQFVDSSTALPVSLDDVARIEVIRGPVSSIYGTNA
                     FFGIINIVTLEADKAPRAYGRTSIDSYGTYGGNAAFNTGTLNRQVRGSVSFRQRQGET
                     LSYPDLENVSSMPTRADGAMSYFGSIAVNYDRLFFQARGYNRERELPGAPYDSQYGSE
                     DNINQDRHGLAELGYTRELTKRVTLAGRIYGNHYTFENDLLLTNGPFSTKTSATWYGG
                     EVRLLADVLEKEKLLSITTGASYELTSTTSSATTKSAPIETDFDIAGAYLEASTEPRP
                     WFGATAGARFDRNSEFTNELSPRAALFLHKGDTYGLKLLYAAGFRNPSVFEAYYDDDL
                     RFAPLLDADMRTGLRPERIKAYELVAYGRPFTGAKLRVSAWEWRLSDLLRRDEVFDPI
                     DNEIRLRYQNTRDLISRGVEIESTYRDLAGRSVYFNAAFSFTGRNCLEDTGVTNIMLD
                     PKVGNCDEPQNSPVVVAQAGASSQRLFELFHLSAETTFISKRGTQRAEALRVNPDDPD
                     GAVPAYVGLNVVAYAPDIRGIDVTIGARNLIMREEAPAQSDYNRAATAMTPRIEVLRV
                     PGPGREVFMRMGYKF"
     gene            12496..13380
                     /locus_tag="VMZ53_25370"
     CDS             12496..13380
                     /locus_tag="VMZ53_25370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31866.1"
                     /translation="MHRIVGGTFRPIIVVNNAAAAAERCMQTPPFLAIVEHELLAATA
                     PFLPRDVQTLVLMQDSEAGELGALLEQGNLSFLLANPMPLLAEELSTTVQKLSRLDIF
                     GLEKYLTWGVEIREIELMNAQNRRELVTEVADDIQRACLGPRPLAAATLIADELLSNA
                     VFNAPVDSSGAHVHANDVRTDSRTLTSRDRVTLRYACDARYLAIEVNDDYGSLDRATI
                     LRCLTKASSRSRDKVSMTTRGAGIGLATVYGSCNHLVFNLDPGKRTQVIALIDVRFRP
                     IELGNTVCSFGIFKTEAP"
     gene            13377..13859
                     /locus_tag="VMZ53_25375"
     CDS             13377..13859
                     /locus_tag="VMZ53_25375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HUS31867.1"
                     /translation="MTTQQKARCTIESTPHGDVLTIAGEIDDTTDLASYGAQLQRNAI
                     IDLGGVTFINSVGVREWVTLLDQVMGRGIRVTLRNVSEPMVRQMTMVMEAKGEANVES
                     FFAPYICTKCGDERALLLELAHHHAALAAGTPPHLPCAKCGAAAEFDEFPARYLAFLT
                     "
     gene            13871..15163
                     /locus_tag="VMZ53_25380"
     CDS             13871..15163
                     /locus_tag="VMZ53_25380"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS31868.1"
                     /translation="MHRLLPMVVFVAACGGNDGPTGPVKVGLLAPKTGALMQVGESFD
                     RVAQVAVDSINGKGGVDGRPLELIVEDTETTADKAGAKLQGLIDMGVVAVVGPATSGE
                     VTNAFPVARDNRVPIISPSSTAPSLSRTSTSGGPDDQGFMFRNVPDDEIQGLAMAYYI
                     KQKREPAVTNVAVVFENSPYGSGLKNSFKTSFEDLGGTVTLEVSFEQNLTDVAAARPT
                     INMLVNAANRPTFVVLVALEQDAVKLVQAWDNNGSPVLPGMQFFMTDGARSAGFLSSA
                     PASVRKMCGTAPTFPIRGLAYRQLQDAYEATFTDKIGDQVFAPNVWDAFHLFAASMVQ
                     QRHAHPDEDIGGEHLRDAITAVSKDGQTFHSGQWRDLIASLRAGNDVDYDGAAGPNDF
                     DVVGQAVGPYEVWCIGDDGASFTQELFLEAKEVQALKP"
     gene            complement(15164..15682)
                     /locus_tag="VMZ53_25385"
     CDS             complement(15164..15682)
                     /locus_tag="VMZ53_25385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31869.1"
                     /translation="MAKRVDHDSTMEVSSSQLVPEGQKRPAPHRAPGQDKNDVSMWIG
                     GVVGADEFAAKRPTAPKGRRWGVVVLLVVLVAVAGGAGYYFFGRSKPPTASSNAVPPD
                     AALVPTATAVPLDAAIDAHTDAAVDAGTDAGALKADAITGVDANKPPAKKPTKKVVKK
                     PLPKKKPAPKRK"
     gene            15736..>17765
                     /locus_tag="VMZ53_25390"
     CDS             15736..>17765
                     /locus_tag="VMZ53_25390"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31870.1"
                     /translation="MRGDVRATYVAPLDPNKAAGLIRAATDRRRIFELVLAGVRSKTR
                     FAAVLSVHTQELRGQFALADAAFDTRPTSELRIPRNVFKRFEAAISSGSPSVGAIVTG
                     EPFVDGYLEALGQPTAHVLVMPILIATRVVALVVAHRGDAELKMEDVSSVFTLVAATG
                     QALSKNLATRTRAAATSGTPVVPADAGYEVEVTDVNAKRTRLATLRSSESWAELASAI
                     RELVREGIEHGEPAEDEQLELLLELGTIELRLGHPDRAIDAWRSAQAIDAADTRVLDA
                     LERAYVQGGQWAEVADLLEKKAALAESVETRTAILLELAAITDERLHDDDRTIAAYER
                     ILSFDPGHHRAGKELEARYSQREDWQALVALLLDRASRHDDPQERVAALMAVANLYES
                     RIGDGRDAFLVWMTVFRREPERPHLIEQLARLADNASHLHEVVAETHALADELAPEHP
                     RVAANLWRLVGTWQRDRMGNRDAAAVAFDHAARLDPDAAVEAAAHLRSDERWPELAAL
                     LTRLLETAPDAKRRAELCCELGELYETHLAQPGEATRWFERAHDHDPDSSAPLVALHR
                     LYLDGKAWYALGELLPQLIDTLGASVPREVIVDLHVELGQILVEHLGRPDEAIEAFRE
                     ALALDGKHEPAFRGLAQVYESTGQTEALLDTSEAEVDAIGGPLQAQRYADIAA"
BASE COUNT         3014 a         5936 c         5749 g         3066 t
ORIGIN      
        1 gcggatggcg cgatatgcag catcggtgcg ggcgccggat gatcgcggaa catcgcgatg
       61 gcgtcggcga gcgagtacgg catcgacggt ccccacggca gcgcgagctt gaccgtgacg
      121 acgagccaga tcgccgcttg ccatgcaggc cgcagcttgc ccgcgcgagc gagcacaaac
      181 gcaagcagcg cgagcagcgt gccttgtgcc gccatcatca gcagcgtcgc gagccacgcc
      241 ttggcgagcg caccggcggt gaagagcgcg gtcatttgcg ctccttcttc ggtttctcgg
      301 gcttctcggg cttctcggcc gccggcttct ccgcgtcggc gatcttgcgc tcgatctcga
      361 tgaggtcctc gcgcgacagg ctctcgtcct cgacgaggcg aagcacgagc gagtcggcct
      421 tgccgtcgaa cacctcgcgc acgaagtcgc gcaggcgatc gcgcagcatc gactcgcggc
      481 ggaatgccgg ccgatacacg aacggctcgc gcgccttgtc gacgacgact gcgttcttcg
      541 tcgcgagccg accgagcagc gtcatcacgg tcgtatacgc gagctcgtta ccgcgtttgt
      601 cgagctcgcc tcgcacgtcg gcgacggagc ccttgccgag ctcccacagc gccttcatga
      661 cgcgcagctc ggcgggggtc agctcgggac acttcatgac gatcgctttc gtaacattac
      721 tacaagtgta gttgcccgag caatgagcct ctctgccggg agcagcggca aaaacgcaaa
      781 gccgaggagc ggatagggaa accagatgtt catgagcaac accacaccga ggtgaaatgc
      841 ccaggctgtg acagcccaca cgtagccgat ccgacggtgc agaagcgcga gcggcgcgcc
      901 gagctcgatg acgagcgtca tcgccgagaa cacgaccagc tgcttgggat gatggagaaa
      961 caggtttgcc agtggcgccg tgggatcgcc gagcaacgcc ttgcggagat tatccacggc
     1021 gatctgattg cgcagcagct cgccatcgat ccaggcgggc cccgcgattc gcagcttcgc
     1081 gatgccggcg agcaggtacg tgagcgtcgt cagcgtcgcc atcaacttca cgggccaacc
     1141 cgcgtcgccc tttggcggtg acagcgccag cgcgatcacg tggagcacga ggagattctc
     1201 ggtgtggaag accatgcccc acgagttgcg gtacgtgatc gtccacaaca ggccgagagc
     1261 ggcgagcggc gcggtgatcc ggtactgcac gttcgcgacg agacatgcga gcagcgcgca
     1321 cgtggcgatc gcgatcgcca gcacgagggc cggcgcgagc ggcgcgtcga gtaatcgcgt
     1381 cacgccgacc tgcgcgaagt gctcgcggcc gtagtgcgcg actttgtaga tttcgggcat
     1441 ccgagcgacg aggtagacga gcgcgtagcc gccgatgagg atgcgcagcg cgcggagccg
     1501 gccctcgggc gcaggcgcga accaccaatt catcgccgca cctcgcagcg cagaagctcg
     1561 ctctcgacgc cgatctcatc atcgccgaga tagcgaaccg cgtcgtgcgt gcctttgacg
     1621 attcgcacaa acgcgatgtc cgtaaaatcg tcgtcggacg cgacgcgctt cgcaatggtc
     1681 tcgcacagcg acgtcaacgc gcccttcccg cctccaaccg cgtgctcgat cacgcgcagc
     1741 gcctgcagga tctcgctgct gccgatcagc gacggcgaca gcgtgcggcg cttgccatcg
     1801 cgagtgacac cgagcgcgta gtgaaacgtc tgcacggtct gccgcttcgt cgcgaacatc
     1861 ggatacgtcg agagcggaaa gccgtcgtcg tacggatcgc ggacgagtgg ctccagcgtc
     1921 gctgcgacga gcgcaagcga caccgcgagg ccgagccacc gtgcgcggtc cattcctcac
     1981 tgttaccatc gctcgatgcg ggaagtccga tcgtgggtag tgatcgtcgt cgcgctagct
     2041 gcgtgtaaag gccagggcgg gaccgcagcg aagggctcgg gcgagatcgc ggcagagggc
     2101 acgaggctcg aaggcgagat tcccgtcgtg ttcggcgatt gcgtcccgaa taccgacaca
     2161 ccgttcgtct ccgggcccga gccgcaagcg ttcacgctcg acgagctgaa gcacgccggc
     2221 aacacgctgt acgtgacgat ggcggacggc ggcgatgtgc acgcagcgtt cgatgggtgg
     2281 gggacgatcg ggaccggcaa gtacggcacg gtcagtcatg gaagcggcgc cggcgccagc
     2341 tacggcatcg gcagcggtcg gctgatcggc cagcgcggaa atggcccgac ggtcacgatc
     2401 ggacagcccg tcgccggcgg cggactcgat aaggcgatca tgcgccgcta catcaagcgc
     2461 aacatccaga agatccagta ctgctacgag aagcagctgc tcgccacgcc agcaatcgcc
     2521 gggacgctca cggcgaagtt cacgatcatc gagaacggca ccgtctcgaa gtcggaggcg
     2581 aacggcgtcc acaaagaggt cgcttcgtgc gtcgcgagcg tgatcaagag catcgaattt
     2641 cccaagccga aaggcggcaa cgtcgaggtg agctatccgt tcacgttccg gccgggtggc
     2701 ggcacgacag ccgcgatcaa gcccgtcgat tcaaccgcgc gtcagcaggc aatcgacgca
     2761 gcgcggaatc agggagtcat cggcgccctc ggcgacagcg gtggagcatt cgcgacgctc
     2821 agcactccgc gaccgaagag caagccgtac caaccgggcg caggctccgc gcttgtcgag
     2881 ctgcgcatcc tgctcgtcga ttgctttcgc aaccaagaca agaactacgc agcggtcgtg
     2941 ttcgatctcg cgccgggtgc gatcaccgcg catggcatcg atcacccggc gttcgcgaag
     3001 tgcattgcgg gcatcgccga acaagtgaaa gtcggcgggc cgctgcgctg ctcggcgacg
     3061 ttcggtactg ccggcgtcga atcgctgcct gccgtcgaca tcaccgcgga tgcgatcacg
     3121 atgttcggca agaaggtcac cgagctcgcg gcagtggtcg gagggcaccc cgagccgtgg
     3181 tggaagatcg agtcggtgtc catcgcagcc agcgagcacg tcaaggcgac gcgacagagc
     3241 aaagccccgc tcacattgca tggcccgctc gccattcgcc cgctggcgac gacgccgatg
     3301 aaggtcgtca cgaacgtcgt gcacaccttg atcgacgccg gggaggatcc tctgctcacc
     3361 gcgcagcgaa acggtacatg gcagctcttg cgcagctatg cgttgcccgt cgttccggtg
     3421 ccgcgcggta ctggcggcag ctgggatagc gaggccaaca tcttcgccga cgaccaagac
     3481 gcagttcatc tctccgtcct cgtcgacaag accgatgtct gggtcggcat atcgcgcacg
     3541 aatgaaggca agcgcgtgcc gctcgcgaag ctcaaagagg agctcgccgc gcacaaggcg
     3601 agcacgcggt tcgccgaacg cgaggacatc gagatcgcgg gcgccgacga ggttacgtat
     3661 ggtcaggtcg tagaggcgat cgacgtcgcg agcgccgccg gattccgcgg ctggtcgctc
     3721 acctcgccgg tcgggctgtc ggcgccggcg cgctgatcac atcgagcgaa cgaccttcca
     3781 cgacgagcgt ggttggccga tctcgaagta gaacgagaac gcgaatgtga tgagcccatc
     3841 gccgttcgcg agctccttcg gcggattcgg gaacggcgct gcggattgga atgcgcgcac
     3901 cgcttcgtca tcgagctcgg acacgcccga cggtgccgtg acgacgatct tggcgagctg
     3961 accgttcgtc gcgagcgcga cgcggacctc ggtgacgcgc gtcttgaagc cgtagacctg
     4021 accgcttgga tcattgcggc gccacaccga cgcggggtcc cagttctgcg cgacctggcg
     4081 cttgaggcgg ttgaagaacg tcgcgtggac ccagcgcttc gaattcaccg ccgtctcgtc
     4141 gccggccagc acgtcttcca tgtgatcgac gttgccgccg ccgagcgcgc gctcgagctg
     4201 ctcttgcgat ggcttcagct gcaggtcggg cacgccgcca ccggcgccgc tctggccctt
     4261 tggaatttcc gagcgctgtt tctgctgctg ctcgaacgcg ccctgcccct tgcgcggcat
     4321 gaagccgtcg aacgcgagcg ggcccatgcc gcccgtggcc gtgccgcgct ccttctgctc
     4381 ttgctcgagc tccgacggag tctgcactcc gggcgtgcgc atcgcgagcg agccaggaac
     4441 atccggcgcg gtcttgttgg cacccttcgc gcggtcttcg tcctgctttt cgatcgatgc
     4501 ttccttcgga ttctgcttcg gcgtcagttc ttcgggctgg ctcttcgcga ccatcggctc
     4561 tttgaccgag ccacgcgcga ccgtctgctt ctctgcgacg gtgttgaact ccgataggta
     4621 gcgcgcgttc tccggctcct tctcggaatt gggtttcgcg gtctcgacga tctgctgcgg
     4681 gcgcttcggc ggaggtggcg ccggcggcgt cggaggcgtg ggctgctgcg gttgcggctg
     4741 ctggatctcg ggcggcttcg gcttgtcctg ctcttgcttc atctcctcga gaagcggctc
     4801 tggatcgatc ggcgtgatct tgtcggcgtt cttctgctcg agcatcgcga tcgctgcaat
     4861 cgcgtcaggc gtgcgacgcg cctcgcagtt cgagaggtcc atccgcatct cgagctcggc
     4921 acctgtgagg cacgcatcgg tgtcctcgcg ccatggagcg aagcacatcg tgtagcgcgc
     4981 agagctcgcg aggagcgcat cgcctacgca gtttgcttgc agctcgacca gctccttggc
     5041 cgtcccgcga tcgcgcgtcg agaatccctc gccgatgacg ttaagatcga agccctcgac
     5101 gctaccgagc aagatggcgt gaaccgccaa cgctgcgacg gcagacgacc acatcttggg
     5161 tcgtgaatat ctccctctgc gcgcgccggt cagcatggga aaaccgagct acccctagat
     5221 tatagaggcg atcgtcgccg cgtcaggtcg attcgacgcc tcgatcgcca ccggaatagg
     5281 ccagttagaa tcggttattg agcgggcgct ggcgccgcag gtgcgacgac gacctcgagc
     5341 ggatcggaga gcggcacgtt gaatgtgcgc cccgtgagca gatcgcggac gttcagccgc
     5401 gccggcggag gcaccgccat ggggccccgc ttggtgttgc tcgaaaacga gcccgccagc
     5461 gaccgatagc tcaccgcgtc ggtgaaccca gagaagccgc cggcgccgcc gaagctcttg
     5521 atgcgcgccg agaatgcggc ggtgagcttg gcgtccgctc ccggcaacgc gacgaacatc
     5581 ggccacccca cattggtgag acccagcagc cgcttcgtgc ccgctggcgg ctcgacgcca
     5641 ctcgacacga acgccgcgtc ggccttccca acggtgatca tcgtcatcgc ggatcgtgcg
     5701 tctgccgcat cgacgatcga gccgaagtac gcggcgtcga cctccgcgcc gagcagcacg
     5761 ttcgtcacga atgcctgctg cttcgcaccg accgtcggga tcgcgacctt cgcgcccttg
     5821 agatccttga tgctgccgat cgacgatttt gcgacgagct gccacggcgc gctcgattcg
     5881 ttgttgcgcg tcgcggtggc gaggatctga tacggcaggc cattggcggc cgcgtacggc
     5941 gcatcgatca ccgcgtattg aatctcgccc ttcttgaccg ctgccgcgaa cgcgctcgca
     6001 ttcgcgtaca ccttgccggt caccttgccg ccattcgcga cgcccttgag gtgatctgca
     6061 atgccgttga tgaagctcac gcgatcgccg gagccttcga acggcgccgt cggtgcgtag
     6121 agtccgatcg tgacgtcatc ggcgcgcgcg agtgtgggca gtgcgagcgt cacggcgagc
     6181 gcaacgagag ctgcgagctt catggcgccc cccagaagcg ttccttcgct tcgatccaat
     6241 ctttgagcgg cgcaaaccgg ctacccgcgg cggcggcggc cttccacgac tgcagcgcct
     6301 tgagtggctc gcccttgcgc tcgtacgcga cgccgatatt gatgcgcgct tcgggcactt
     6361 caccgacgag gtttgcgagg acgttcatcg cgttgtccgt ttggccaccg acgagatcga
     6421 tcaccgcgag gtcatgcacg acttcggggc tgcgcttgtc gtagctcttc gcggtcgcaa
     6481 gaaactgtgc ggcgagcttc gtattgcctg cggtgtacgc ctcggccgcg gcgcgcacgg
     6541 cgatatctgt tgcggcggtg cgcaggagcg gctcggcgac gccagtcgcg cgacggcgca
     6601 tcgtctcgag acggtcgagc gtcttgcgtg cgcgcttggg cgacgcatcc tcgttccacg
     6661 cgatcaagat cggcacgccg acgtcgtcgg ccggcgcgac gaacggacac ttcgcctttc
     6721 cgcgttcgag cccgcgcagc agatccagtg cgaggtcgcg ttgctgagca cctgtcgcgg
     6781 cgagcgcgag gtcgcaccgc agatcgacga gcgcgtcacc gtctgccgac ttctcgacgg
     6841 agcgcaggat cttcaccgcg accgagaatc gcgctgcacg catcgcctcg gttgcggcac
     6901 ttcgagccac cgcaaggcgc gcacgatcga gctccttctt cgtaccagcc ggagcggtct
     6961 gcacggcggc gtcgagcacg gcgactgctt cttcgccgcg accggccgcg agctcggatg
     7021 ctgcgaggtc acgctgaacg accgcgacgc gtgcatcctt gccgtagagc ttgatcgcgc
     7081 ggccgtacgc gttgcgtgcg tcgtcgaaac gcttcgctgc ctgcagcgct cgacccagca
     7141 cgtattgcgc gtccgcgtcg ttcgcgtcgg ccttttgcag cacttcgagc gcgcgcggca
     7201 ggtcgttctt cgcgacgagc actgccccga ggttgcgtgt cgtcagcgtg ctgccacccg
     7261 ccgcggcggc cttgtcgagg aagctcgcgg cttgcgcgag atcctttttc ttcaccgctt
     7321 ctgctgcgag cgtgttgagc gcatcgacga acgtcggcgc tgcacgttgt tcgccttgat
     7381 cgagtgcggc atcgaggtga ccgaccgcct gcgcgtgatc gccgaggatg tagagcgccg
     7441 agccgaggag gaactgcgcc ttgccattct tctcgtgggc agcgacaaac ggcgccacga
     7501 gctctttgag cgcagcagcg cgccgaagca cgagcagcgt ctcgccgtac tcgagcgtcc
     7561 agtcgtcctc gtaatccttc ggcggtcccg ctgctttgag gcggtccgca gccgccgcgg
     7621 tcttgccgag atgacgttcc gcacgcgcga gctcgagcgc gaacaaccga tcgcttggcg
     7681 atgcctcgac gacgtgcaga tacgcggcct cggcttcctt ccagttgttc tgcgcagcgc
     7741 ggacgtcacc gagcaggatc cagccggcga gcgcattcgg cgagctcttg cgaaaccgct
     7801 ccgcgcgatt gaccgcgtcg tcgagccgcc cgagcttgag tgcgcagcgt ccgcgcacga
     7861 gcgagatttc ggcgtacttc gcctcgagct cgccgaggtt gtcgaggatc ggcatcgcct
     7921 gcttgcacga gatcgcagcg gttgcccatg caacaccgag gcgcgcgcgg tcgtctttct
     7981 tgtcttcgcc gagcttcttg tacagatcga gcgcgccctt ggcgtcgccg ctgcgcagca
     8041 tcgagttcgc ctgatcgtag cgatgcagcg agtcctcgcc ggcgagcgcc ttcggccggt
     8101 tcgcgaagta cgcatcgaac gcttttgcag cgtccttcca ctcgccttgc ttcgcgtagt
     8161 agtggccgag cacgacctgc gcatcgaagt gacgcgcatc gccggcgacg atcttctcga
     8221 gaatcggcac gccttcgctg ccgcgcccga cctcgacgag ctcgacgccg agcgagatgt
     8281 tcacgcgcgt gttgttcggc gacagctgac gagcgcgctc gagcagcgtc accgccgcgt
     8341 cgtgcttgcc gacgatgccg tagatgctcg cgcgcaagat gtagagatcg atgcggcgcg
     8401 gcgacgagac cagcgccttg tcgagcaagc cacccgctcg ctcgtaatcc ttcttggaga
     8461 gagcgcgttt cgcttcttcg acgagcagct ggctctcttc atcttgctcg cgctgcgcga
     8521 atgccggtgt cgtcgtcgcg aacgcgagca acgccgcgag cacgatgcgc ttcacgcagc
     8581 ggctccccgc cccgcattga ttcccttgcg cgagcccgta gccgtaccgg agtactggac
     8641 gatgacgagc gtgatgtcgt cgtcgactgg ctgttcgcca ccgaagcggt tcacttcgcc
     8701 gaccacgcga tcgcacaacg cgcgcacgtt gttcgtaccg cgcgtgactt cttccatgag
     8761 cgccttgcgc aggcgcttgt cgccgaagcg cgtgccttcg cgcgagacgc gatcggtgag
     8821 gccatccgac gtgaggacga tgaagtcgcc gttcgcgagc tggcacttgc cctcggcgat
     8881 gtgcgggcga tacgagccga gcgggttcga acgcgtcggc aggatctcga gcatcggctg
     8941 cttcttcgtg tcggagatga cgtacgggaa cgtgtggcct gcgttcgcgt agtcgacgac
     9001 gccgtcttgg cgcaggatca tcgcgaagca ggtcatcaac agtcgctcgc cgacatcgcg
     9061 gatcgctttg tcgatcgcgc gcagcacgag ctcgggcgtg atcgcgttcg aattgatgaa
     9121 cgccagcgat tcgaccgcgc cgcgacctgt cgccgcgatc atcgcgctcg gcatgccgtg
     9181 gcccgtcacg tcacctacga cgagcaacaa gcggccatcc gcgagttggc gatagctcca
     9241 ccagtcgccg ccgcacgccg acgccatctc gcagcgaccg atcaagttga acgaatcgag
     9301 cgcaaccgct tcgggcggcg gcgtcatcag gccttgaatg ctgcgcgcga cttcgagctc
     9361 gcgctcgagg ctcgccttgc ggccgatttc ttcgacgaga tagacgaggc tctccgacat
     9421 ccggttgaag ctctcgccga gctggccgac ctcatcaccg gtcttcacga ccacgcgcct
     9481 cccgaaatca ccggcggcga tgtggcgagc ttgctccgac agtgcgagca gcggccgcga
     9541 gattcgcagc gcctgccaca gcgcgagtag caaaccgatg gctagcaata agccggcgaa
     9601 cagcagctgc cgctctgccg atgcgcgcgc acgttcgcga ccggccgcga gcgcttcttg
     9661 ctgcgcgcgc tccatcgcag acatgtcgaa cgcgagacgc acggtaccga cgcgaacttc
     9721 tttgcccgtg ctgtccggaa tgaacaccgg cgtgccgagc aagagatgcg acttgtcggc
     9781 gggatcggga atctgttcga ccttgccact cgccgcggtc tcgagagtct tcgcgagcgg
     9841 atcgtcgatc ttgccgccct gtttcgccgg cgccttgtcg ctcgccgcgg ccacagtgcc
     9901 gctcggctcg acgagcgcga tccacacgac gttgcggttc tcgacggcaa gattgcgcgc
     9961 aatctcctcg agccgcgtgt agtcgctcgt cgcgagcagc gatgcggacg tcgctgatgc
    10021 attgcgcgtc agcagctccg cttcgcgttg aaatgctttc tcgagatcgt tgcgccgcgc
    10081 ttcaccgctt gcgcgcgcgt agctatcgag cgagttgagc ccgtaccacg cgctcgaagc
    10141 gacagctgtc gcgatgagca gcgcagttcc taaggtcagc ttcgtaccga gcgataggcg
    10201 gcggcgcacg agtgttccag ctcaggaaca gtctaagcca aaacgagttg acgtcggcgc
    10261 gaggcggccc tcaaaatggc gtctcgtgca ctggtcacgc gttgggatcg cggcggtcgc
    10321 ggtacccctc atgcttagcg gtgtggccgg ggcggatgac gaacccgcga tcccgattcg
    10381 cgagcttccg ccttttcctg atgtcgaggc gagcgcgcaa gtctccgaag tcggcgtcta
    10441 cggcgcgagc caatcggagg aagagaccgt cgtcggcgcg gcgaagcgcg agcaatcgct
    10501 cggcactgtg gcctcggcgg tcacggtcct caccgcggat cagatgcgca gattcggcta
    10561 tcgctcgctc gccgaagcgc tgcgcggcat ctccggtgtg tacgtcgtcg acgatcgcat
    10621 ggtcgaacgc atcggcgtcc gcggccttca gctgctcggc gattcgaaca cgcgaatcct
    10681 gatcctcatc gatggcacgc cgctcaacga gccgtgggcg cagttcgtcg acagctcgac
    10741 cgcgctgccg gtgagtctcg acgacgtcgc gcgcatcgag gtcattcgtg gccccgtgtc
    10801 gtcgatctac ggcacgaacg cgttcttcgg catcatcaac atcgtcacgc tcgaagcgga
    10861 caaagcgccg cgcgcgtacg gccgcacatc gatcgacagc tacggcacgt acggcggcaa
    10921 cgctgcattc aataccggca cgctcaatcg ccaagtgcgc ggcagcgtct cgttccgcca
    10981 acgccaaggc gagacgctct cgtatccgga tctcgagaac gtctcgtcga tgccgacgcg
    11041 cgccgacggt gcgatgtcgt acttcggctc gatcgccgtc aactacgatc gcctcttctt
    11101 ccaagcgcgt ggctacaacc gcgagcgcga gctgcccggc gcgccatacg acagccagta
    11161 cggcagcgag gacaacatca accaagaccg ccacggcctc gcggagctcg gctacacgcg
    11221 tgagctcacg aagcgcgtca cgctcgctgg ccgcatctac ggcaatcact acacgttcga
    11281 gaacgacctg ttgctcacga acggcccgtt ctcgacgaag acgagcgcga cgtggtacgg
    11341 cggcgaagtt cgcttgctcg ccgacgtgct cgagaaagaa aagctgctct cgatcacgac
    11401 cggcgcttcg tacgagctga cctcgacgac gtcgagcgcg acgaccaagt cagcgccgat
    11461 cgagacggac ttcgatatcg caggcgcgta cctcgaagcg tcgacggagc cgaggccgtg
    11521 gttcggcgcg acggccggcg cacgcttcga ccgcaactcc gaattcacga acgagctatc
    11581 gccgcgcgcc gcgctcttcc ttcacaaggg cgacacgtac ggcttgaagc tgctgtacgc
    11641 cgccggcttc cgcaatccga gcgtgttcga ggcgtactac gacgacgatc tgcggttcgc
    11701 gccgctgctc gatgcggaca tgcgaacggg tctgcgtccc gagcgcatca aagcgtacga
    11761 gctcgtcgcg tatggccgtc catttaccgg cgcgaagctg cgcgtctcgg cgtgggagtg
    11821 gaggctgtcg gacttgctcc gtcgcgatga agtctttgat ccgatcgaca acgagatccg
    11881 acttcgctac cagaacacgc gcgacctcat ctcgcgtggc gtggagatcg agagcaccta
    11941 tcgcgacctc gcagggcgct cggtgtactt caacgccgcg ttctcgttta ccggccgcaa
    12001 ctgcctcgaa gacaccggcg tcacgaacat catgctcgat ccgaaggtcg gcaattgcga
    12061 tgagccgcag aactcgccgg tcgtcgtcgc gcaagccggc gcgtcatcgc agcgcctctt
    12121 cgagctgttc catctctccg cggagacgac gttcatcagc aagcgcggca cgcagcgcgc
    12181 cgaagcgttg cgcgtgaatc ccgacgatcc tgacggagct gtgcccgcat acgtcggact
    12241 caacgtcgtg gcttatgcgc ccgacatccg cggcatcgac gtcacgatcg gcgcgcggaa
    12301 cttgatcatg cgcgaggagg cccccgcgca atccgattac aaccgcgccg caacggcgat
    12361 gacacctcgc atcgaggtgt tgcgcgtccc gggccctggt cgcgaggtgt tcatgcgcat
    12421 ggggtacaag ttttgacggc acgtgatctt cggccgattc tcgtcgtcca cccggacacg
    12481 aagacgcagc gaatcgtgca ccgcatcgtc ggcggcacgt tccgcccgat catcgtcgtc
    12541 aacaacgccg ccgccgcggc agaacgctgc atgcagacgc cgccgtttct cgcgatcgtc
    12601 gagcacgagc tgcttgcggc gaccgcgccg ttcttgccgc gggatgtcca gacgctcgtg
    12661 ctcatgcagg actccgaagc gggtgagctc ggcgcgctgc tcgagcaagg caatctgtca
    12721 ttcctcctcg cgaacccgat gccgctgctc gccgaagagc tgtccacgac ggttcagaag
    12781 ctctcccgtc tcgacatctt cgggctcgag aagtacctga cgtggggcgt cgagattcgc
    12841 gagatcgagc tgatgaacgc gcaaaatcgc cgagagctcg tcaccgaggt cgccgatgac
    12901 atccaacgcg cgtgcctagg cccccgccct cttgcggccg cgacgctcat cgccgacgag
    12961 ctgctctcga atgccgtgtt caacgcgccc gtcgacagca gcggcgcgca cgtccacgcc
    13021 aacgatgtcc gcacggactc gcgcacgctg acgagccgcg atcgcgtcac gctccgctac
    13081 gcctgcgatg cgcgctacct cgcgatcgag gtcaacgacg actacggctc gctcgaccgc
    13141 gccacgatcc ttcggtgcct gacgaaagcg tcgtcgcgtt cgcgcgacaa ggtgtcgatg
    13201 acgacgcgcg gtgcaggtat cgggctcgcg acggtctacg gcagctgcaa ccacctcgtc
    13261 ttcaatctcg atcctggcaa gcgcacacaa gtgatcgctc ttatcgacgt ccgtttccgt
    13321 ccgatcgagc ttggtaatac cgtctgctcg ttcggcatct tcaagaccga ggcaccatga
    13381 cgactcagca gaaagcgcgt tgcacgatcg aatcgacgcc ccacggcgac gtgctcacga
    13441 tcgccggcga gatcgacgac acgacggacc tcgcgagcta cggcgcgcag ctccaacgca
    13501 acgcgatcat cgacctcggc ggcgtgactt tcatcaactc ggtcggcgtg cgcgagtggg
    13561 taacattgct cgaccaagtg atggggcgcg gcattcgcgt cacgcttcgc aatgtgtcgg
    13621 aaccgatggt gcgccaaatg acgatggtga tggaggctaa gggcgaagcg aacgtcgagt
    13681 cgttcttcgc gccgtacatc tgcacgaagt gcggcgacga acgcgccctg ctcctcgagc
    13741 ttgcccacca tcacgccgca ctcgccgccg gcacaccgcc tcacctgccg tgcgcgaaat
    13801 gtggcgccgc cgcggagttc gacgagttcc ccgcgcgcta cctggcgttt ctcacgtgaa
    13861 aggagcaccg atgcatcgtc tccttccgat ggtggtgttc gtcgcagcgt gcggcggtaa
    13921 cgacggcccg accggccccg tgaaagtcgg actgctcgcg cccaagaccg gcgcactgat
    13981 gcaggtcggc gagtcattcg atcgcgtggc tcaggtcgcg gtcgacagca tcaacggcaa
    14041 gggcggcgtc gacggccggc cactcgagct gattgtcgag gacaccgaga ccacggccga
    14101 taaggccggc gcgaagctgc agggcttgat cgatatgggc gtcgttgcag tggtcggccc
    14161 ggcgacgtca ggcgaagtga cgaatgcgtt tccggtcgcg cgcgacaaca gagtgccgat
    14221 catctcgccg agctccaccg cgccttcgct ctcgcgcacg tcgacgagcg gcggccccga
    14281 cgaccagggc ttcatgttcc gcaacgtgcc cgacgacgaa atccaaggcc tcgcgatggc
    14341 ctactacatc aagcagaagc gcgagcccgc cgtcacgaac gtcgctgtcg tgttcgagaa
    14401 cagcccgtac ggcagcggtc tcaagaactc gttcaagacg tcgttcgagg atctcggcgg
    14461 caccgtcacc ctcgaggtca gcttcgagca gaacctcacc gacgtcgctg cagcgcggcc
    14521 gacgatcaac atgctcgtga acgcggcgaa ccggccgacg tttgtcgtgc tcgtcgcgct
    14581 cgaacaagat gccgtcaagc tcgtgcaggc gtgggacaac aacggctcgc cggtccttcc
    14641 aggcatgcag ttcttcatga ccgacggcgc gcggtcggcg ggcttcttgt cgtcggcgcc
    14701 tgcgtcggtt cgcaagatgt gcggcacggc gcccacgttt ccgattcgcg gcctcgcata
    14761 tcgccagctc caggacgcgt acgaggcgac gttcacggac aagatcggcg atcaggtgtt
    14821 cgctccgaac gtgtgggatg cgttccatct ctttgcggcg tcgatggtgc agcagcgcca
    14881 tgcgcatccc gacgaggaca tcggcggcga gcatctgcgc gacgcgatca ccgcggtctc
    14941 caaagatggc cagacgttcc actcggggca atggcgcgac ctcatcgcgt cgctgcgcgc
    15001 tggcaacgat gtcgactacg acggcgctgc aggcccgaat gacttcgatg tcgtcggcca
    15061 agccgtgggc ccgtacgaag tctggtgcat cggcgacgac ggcgcgtcgt tcacgcagga
    15121 gctgtttctc gaagcgaaag aagtccaggc gctcaagccc tgatcacttc ctcttcggcg
    15181 cgggcttctt cttcggcagc ggctttttca cgaccttctt ggtcggcttt ttggccggcg
    15241 gcttgttggc gtcgacgccg gtgatcgcat cggccttcag cgcgcctgca tccgtcccgg
    15301 cgtctaccgc cgcgtcggtg tgcgcatcga tcgcagcgtc gagcggtacc gcggtcgctg
    15361 tcggcacgag cgcagcatcg gggggaaccg cgttcgacga tgcggtaggc ggtttcgagc
    15421 ggccgaagaa gtagtagccc gcgccgcccg cgaccgcgac gagcacgacg agcaggacca
    15481 cgacgcccca gcgccggccc ttcggtgcgg ttggccgttt cgccgcgaac tcgtcggcac
    15541 cgacgacgcc gccaatccac atgctgacgt cgttcttgtc ctgcccgggc gcgcggtggg
    15601 gcgcagggcg cttctggccc tccggaacta gttggctaga cgacacttcc atcgtcgagt
    15661 cgtgatcgac gcgctttgcc atctgcgcac gagtctactc gcacccggag gtcacgtggc
    15721 gataggatcg cactcgtgcg tggggatgtc cgtgcgacct atgtcgcacc gctcgatccg
    15781 aacaaagccg cgggattgat ccgcgctgcg acggaccggc gtcgcatttt cgagctcgtg
    15841 ctcgcgggcg ttcggtcgaa gacgcggttc gcggcggtcc tctccgtgca cacgcaggag
    15901 ctgcgcggcc agttcgcgct cgcggacgct gcgtttgata cgcgcccgac ctccgagctg
    15961 cggatcccgc gcaacgtctt caagcggttc gaagcggcga tttcatcggg ctcgccgtcc
    16021 gtgggcgcga tcgtcacggg cgagccattc gtcgacggtt atctcgaagc gctgggccag
    16081 ccgacagctc acgtgctcgt gatgccgatc ttgatcgcca cgcgagtcgt cgcgctcgtc
    16141 gtcgcgcatc gcggtgatgc cgagctgaag atggaagacg tctcgagcgt gttcacgctc
    16201 gtggcggcga ccggacaagc gctatcgaag aacctcgcga cacgcacccg agccgcggct
    16261 acgtcaggca cgcccgtcgt tcccgccgac gctggttacg aggtcgaggt caccgatgtc
    16321 aacgcgaagc gcactcgcct cgcgacgctg cgatcgagcg aatcgtgggc ggagctcgcg
    16381 agcgcgatcc gagagctcgt gcgcgagggc atcgagcacg gcgagcccgc cgaagacgag
    16441 cagctcgagc tgctgctgga gctcgggacc atcgagctgc gcctaggtca tcccgaccgc
    16501 gcgatcgatg cgtggcgaag tgcgcaagcg atcgatgccg ccgatacgcg cgtgctcgac
    16561 gcgctggagc gcgcgtacgt ccagggcggc cagtgggcag aggtcgccga cctcctcgag
    16621 aagaaggcag cactcgccga atccgtcgag acgcgaacgg cgatcctcct cgagctggcc
    16681 gcgatcaccg acgagcggct gcacgacgac gaccgtacca tcgccgcata cgagcgcatc
    16741 ctctccttcg atccaggtca ccatcgcgcg gggaaagagc tcgaggctcg ctatagccag
    16801 cgcgaggact ggcaagcgct cgtcgcattg ctgctcgacc gcgcgtcgcg gcacgacgat
    16861 ccgcaagagc gcgtcgcagc gttgatggcc gtcgcgaatc tctacgagtc gcggatcggc
    16921 gacggtcgtg atgcgttcct cgtgtggatg acggtgttcc gtcgcgagcc ggagcgcccg
    16981 catctgatcg agcagctcgc gcgtctcgcc gataacgcga gccacttgca cgaggtcgtc
    17041 gcggagacac atgcgctcgc cgacgagctg gcgcccgagc atccgcgcgt cgcggcgaac
    17101 ctgtggcggc tcgtcggaac atggcagcgc gatcgaatgg gcaaccgcga tgcagctgcc
    17161 gtcgcgtttg accatgctgc tcgcctcgat cccgatgcgg ccgtcgaagc cgcggcacat
    17221 ctgcgcagcg acgaacggtg gcccgagctt gccgccctgc ttacgcgctt gctcgagacg
    17281 gcgccggatg cgaagcgccg cgcagagctg tgctgcgagc tcggcgagct gtacgagacg
    17341 cacctcgcgc agccgggcga agccacacga tggttcgagc gcgcgcacga tcacgatccg
    17401 gactcgtcgg cgcccctcgt cgcgctgcac cggctctatc tcgacggcaa ggcgtggtac
    17461 gcgctcggcg agctgctgcc acagctgatc gacacgctcg gtgcaagcgt gccgcgagaa
    17521 gtcatcgtcg atctgcacgt cgagctcggc cagatcctcg tcgagcatct cggtcggccc
    17581 gacgaagcga tcgaagcatt ccgcgaggcg ctcgcgctcg atggcaaaca cgagccagcg
    17641 ttccgcggtc tcgcgcaggt ctacgaatcg acgggccaga ccgaagcgct cctcgacacg
    17701 agcgaagccg aggtcgacgc gatcggcggt cctctccaag cgcagcgcta cgccgatatc
    17761 gccgc
//
LOCUS       DATLBZ010000503         5617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9596475, whole genome shotgun sequence.
ACCESSION   DATLBZ010000503 DATLBZ010000000
VERSION     DATLBZ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9596475"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VMZ53_25395"
     CDS             complement(<1..1246)
                     /locus_tag="VMZ53_25395"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS31871.1"
                     /translation="MSRRLVLIAAAVALAIAGVIVWRVTRKTQAHHPKVAPLRPAEKP
                     AAKTDKISIKGRLFDEEKGAPIAAVKVVLLGAAGEESATTSADGGFGFTVPPGRYRVF
                     VRDLDWITAGLQERIRIDDGPDAKFANVLDAHIAPLLVADTDLDMLEITATKAAKVSG
                     RVADDDGNLVVGAAVRLRQPPTRSSSLRPVLGTDTAITNDAGEYTLAVPPGMYEMELR
                     HPKYAGMVGAVAGLNLGAGVRDTESLVAVKGCIVRGKVVMADGTTPAPDGAVEVDNTG
                     GTSFGPMARVALDGTFEFATTATGYLRVRAWPWKSTPSQTQEVDCYNGKRYDHITLKL
                     EERPPDLAGTLVDAQGRPMPFRHVDLQNLDPGVTGQQERTDAKGEWHFYDVEDGNYRL
                     IATAEGRGIADLNIRSPKTDIQL"
     gene            complement(1274..2032)
                     /locus_tag="VMZ53_25400"
     CDS             complement(1274..2032)
                     /locus_tag="VMZ53_25400"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HUS31872.1"
                     /translation="MARTDVGRKRQINEDAFFRDDARGFYVVADGVGGHNKGEIASRE
                     ATEQLKSWVYGASRDLDRLVERVAMGDSECVWEIRKLLETGVKQACYMIYSMAEQDPE
                     KKGMSTTLSALLIRANYAFAAHVGDSRVYRVRKGNVLQLTEDHTLINYKVKHGMMTKE
                     EAEKATGKNVITRAVGHKDYVQVDTADVDVANSDRFLLCSDGLHGYFNDNDREVAELC
                     ADGELEECADACIALANQRGGKDNITAVVVEVLA"
     gene            2109..2942
                     /locus_tag="VMZ53_25405"
     CDS             2109..2942
                     /locus_tag="VMZ53_25405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31873.1"
                     /translation="MSVGPQLAAVLRERASEILAAWIVRFERSPLRFRRAIKAATHTA
                     QVASLLEALTEAAHGGADALKPGSDATRDLERSCAFMGAQFAGEGATGYDVAALLLEL
                     RDVVDGIAEPADKLAVTRCFEWLTVVALDGFAASGLQSLREQTSEQLDAGTPVIEVLP
                     KVPAVLLVGAPSGGVIDSLLARAWMLAVGTGAPCLIIDCGGLAAQGEKNFERGYRGFL
                     EQAEGSALQVLLSSARRPLRELAAKLTAESNLSFQHFDRFDSAIAHALERAGHLLMRR
                     S"
     gene            2975..5182
                     /locus_tag="VMZ53_25410"
     CDS             2975..5182
                     /locus_tag="VMZ53_25410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31874.1"
                     /translation="MGDFPVGFGRYQLVERLAMGGMAELFVATSPGEHGFQKKVVIKR
                     LLPHLSTDDTYKEMFIDEAKLTARLVHPKIAQTFELGKVDNSLFIAMEFVDGIDVLAL
                     LREYATRRRKIAPELAAYICHEVLDALDYAHNAKDENGNSLGIVHRDISPSNVLLSVR
                     GDVKLVDFGIARLIDPDRAHKSKTGTLKGKYGYMSPEQVIEMPLDGRSDVFSVGVVLA
                     ELLTGRRLFAAANELDVLLMVRDAKLGRLDKYGADIEPGLAAIVRKALKKPVDERWAG
                     AAAFRDAIGEWLFEHRHRMTPKNIAEIVGELREAVNERRIKSAAEGNAQTAVLEDVVQ
                     SRVPSENLESIPVLSMDDQDVSMPVISVSYDAEQPDPPSRPVTSDPIPASKVPAAAAP
                     PVIAPVKAPPRDRSAPIVIPVKHDRSAKEDKDAAPEAPIADSISAAVGAIVLPDDEER
                     AREDSKPHSFDDLTEEPAAMRSRDIQMPTAEELARKKPPTPPSLADIEEAPDDAGDFA
                     LTSPLRVLFRLMTARATGLLVVAVGGIKKEIYVRDGQPEYVSSNVASELFGNYLVTKG
                     VLSDGELAMALAMMPHYGGKLGDTLVGLGLLKPLEVFRHLTRQVRTKIIDVCTWNKGG
                     FAWYAGRENPREAFPLDFNAFEILGAGAMALDPDVVDTWIAKSSSVRLRASRARRVGP
                     ERFEVKGLVELFENLDGKTTIGGLVDAQPDREERQRTARMLCLLEACDLARPA"
     gene            5202..>5617
                     /locus_tag="VMZ53_25415"
     CDS             5202..>5617
                     /locus_tag="VMZ53_25415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31875.1"
                     /translation="MNASTTVKAGDTLEGRYKILNLVGEGGMGKVFLAEHMLIKRRVA
                     IKILRPELATDSNVVERFMNEARAAGAIGHPHIVESTDMGFTREEVPFIVFEYLEGSL
                     LTDEIYRTKGMSPRRALRIATQIASALHAAHQAGIV"
BASE COUNT          952 a         1853 c         1808 g         1004 t
ORIGIN      
        1 ggagctggat gtcggtcttc ggcgagcgaa tgttgaggtc cgcgatgccg cgcccctccg
       61 ccgtcgcgat cagccggtag ttgccgtcct cgacgtcgta gaagtgccac tcgcctttgg
      121 cgtccgtgcg ctcctgttgc cctgtaacac ccggatcgag gttctgcaga tcgacgtgcc
      181 tgaacggcat tggccggccc tgcgcgtcga cgagcgtgcc agccagatca ggcgggcgtt
      241 cttcgagctt cagcgtgatg tggtcgtagc gtttgccgtt atagcagtcg acttcttgcg
      301 tctgcgacgg cgtcgatttc cacggccacg cgcgcacgcg aaggtaacca gtcgccgttg
      361 tcgcaaactc gaacgtaccg tcgagcgcga cccgcgccat cgggccaaag ctcgtcccgc
      421 cggtgttgtc gacctcgacc gctccgtcgg gtgctggcgt ggtgccatcg gccatgacga
      481 ccttgccgcg cacgatgcag cctttcaccg caaccaagct ctcggtgtcg cgcacgcccg
      541 cgccgagatt gaggccggcg acggccccga ccatgccggc gtacttcgga tgccgaagct
      601 ccatctcgta catcccgggc ggcacggcga gggtgtattc gccagcatcg ttcgtgatcg
      661 cggtgtcggt gccgagcacg ggacggagcg acgagctgcg cgtcggaggc tggcgcaaac
      721 gcacggctgc gccgacgacc agattgccgt catcatcggc gacgcggccg ctgactttcg
      781 ctgccttcgt cgcggtgatc tcgagcatgt cgagatcggt gtcggcgacg agcagcggcg
      841 cgatgtgcgc atcgagcacg ttcgcgaatt tcgcgtccgg tccgtcatcg atgcgaatgc
      901 gctcttggag accggcggtg atccaatcga gatcgcgcac gaacacgcga tagcggcccg
      961 gcggcaccgt gaagccaaag ccgccatcgg cgctcgtcgt cgcagactct tcgccagcgg
     1021 cgccgagcag cacgaccttc acggctgcga tcggcgcacc cttttcttcg tcgaacagcc
     1081 ggcccttgat cgagatcttg tcggtcttcg cggctggctt ttcagcaggg cgcagcggcg
     1141 cgaccttcgg atgatgcgcc tgggtcttgc gcgtcactcg ccagacgatc acgccggcga
     1201 tcgcgagcgc gactgcagcg gcgatcagga cgagccggcg agacacgaga tcgacgtacc
     1261 accgcgcgag cggctacgcc aggacctcga cgacgactgc ggtgatgttg tccttgccgc
     1321 cgcgctgatt ggcgagcgcg atgcacgcgt ccgcgcactc ttccagctcg ccgtcggcgc
     1381 acagctcggc aacttcgcgg tcgttgtcgt tgaagtaacc gtgcaggccg tcgctgcaga
     1441 gcaagaagcg atcgctgttt gcgacatcga cgtccgcggt gtcgacttgc acgtagtcct
     1501 tgtggccgac ggcgcgcgtg atgacgttct tgccggtcgc cttctcggct tcctctttcg
     1561 tcatcatgcc gtgcttcacc ttgtagttga tcagcgtgtg atcctcggtg agctgcagga
     1621 cgttgccctt gcgcacgcga tacacacgcg agtcaccgac gtgcgcagcg aacgcgtagt
     1681 tcgcgcggat gagcagcgcc gacagggtcg tcgacatccc cttcttctcg ggatcctgct
     1741 ccgccatgct gtagatcatg taacaagctt gcttgacgcc cgtttcgagc agcttgcgga
     1801 tctcccacac gcactcggag tcgcccatcg cgacgcgctc gacgaggcga tccagatcgc
     1861 gcgaggcgcc gtacacccac gacttgagct gctcggtggc ctcgcggctc gcgatctcgc
     1921 ccttgttgtg accgccgaca ccgtcggcga cgacgtaaaa gccgcgcgca tcatcgcgga
     1981 agaaggcgtc ctcgttgatc tgacgcttcc gcccaacgtc tgttcgcgcc acgctaagta
     2041 gcttcaaagt gccacccgtc gcaaatcgta acatggcaag ccccggatcg gactgctacg
     2101 ctgccagggt gagtgtaggg ccacagctcg cggcggtttt gcgtgaacgc gcgagtgaaa
     2161 ttctggcggc gtggatcgtt cggttcgagc ggtcgcccct gcgatttcgt cgtgcgatca
     2221 aggctgccac tcacacggcg caggtcgcga gcctcctgga agcgctgacg gaagccgccc
     2281 acgggggcgc cgatgcgctg aaacctggtt cagacgcgac tcgcgatctc gaacgcagct
     2341 gtgcgttcat gggcgcacaa ttcgctggtg aaggtgcaac gggctatgac gtcgccgcac
     2401 ttctcctcga gctccgcgat gtcgtcgacg ggattgccga gcccgcagac aagctcgcgg
     2461 tgacccgctg cttcgagtgg ctgacggttg ttgccctcga tggcttcgcg gcgtcgggcc
     2521 tgcagtcgct gcgcgagcag acgagcgagc agctcgatgc cggcacgcca gttatcgaag
     2581 tgctgccgaa ggtgcccgcc gtgctgctcg tgggcgcacc ctcgggcggc gtgatcgaca
     2641 gtttgctcgc acgcgcatgg atgctggccg tcggcaccgg cgccccgtgc ttgatcatcg
     2701 actgcggagg cctcgccgcg cagggtgaga aaaacttcga gcgcggctat cgaggattct
     2761 tggagcaagc agaaggcagc gcgctgcagg tgttgctgtc atccgcgcgc aggccgctcc
     2821 gcgagctcgc cgcgaagctc accgccgaga gcaatctgtc gttccaacac ttcgatcgat
     2881 tcgacagtgc catcgcgcac gcgctcgaac gcgctggcca cttattgatg cgaagaagct
     2941 gagttacctc gcaccgtgct aagcttttcg tccggtggga gacttcccag tcggctttgg
     3001 ccgataccag ctggtcgagc gccttgccat gggtggcatg gccgagctgt tcgttgcgac
     3061 cagccctggt gagcacggct ttcagaagaa agtcgtgatc aagcggctgc tgccgcatct
     3121 ctccaccgac gacacgtaca aagagatgtt tatcgacgag gcgaagctga ccgcgcgcct
     3181 ggtccacccc aagatcgctc agaccttcga gctcggcaaa gtcgacaact cgctgttcat
     3241 cgcgatggag ttcgtcgacg gcatcgacgt gctcgcgctg ctgcgggagt atgcgacgcg
     3301 ccgccgtaag atcgcgccgg agctcgctgc gtacatctgc cacgaggttc tcgacgcact
     3361 cgactacgcg cacaacgcga aggacgagaa cggcaactcg ctcggcatcg tgcatcgcga
     3421 catctccccg tcgaatgtct tgttgtccgt gcgcggtgac gtgaagctcg tcgacttcgg
     3481 catcgctcga ttgatcgatc cggatcgcgc ccacaagagc aagacgggca cgctcaaggg
     3541 caagtacggc tacatgtcgc ccgagcaggt gatcgagatg ccgctcgacg gacgcagcga
     3601 cgtgttctcc gtcggcgtcg tgctcgccga gctgctcacc ggtcgtcgct tgttcgccgc
     3661 cgccaacgag ctcgacgtcc tgctcatggt gcgcgacgcg aagctcggtc gtctcgacaa
     3721 gtacggcgcc gatatcgagc cgggcctcgc cgcgattgtt cgcaaggcgc tgaagaaacc
     3781 cgtcgatgaa cgctgggccg gcgcggctgc gttccgcgat gcgatcggcg agtggctgtt
     3841 cgagcaccgc caccgcatga cgccgaagaa catcgcggag atcgtcggtg agctgcgcga
     3901 ggccgtgaac gagcggcgga tcaagtcggc cgcggaagga aacgcgcaga cggcggtgct
     3961 cgaggatgtc gtccagagtc gcgtgccgag cgagaacctc gagtcgattc cagtcctctc
     4021 gatggacgat caggacgtca gcatgccggt gatctcggtg agctacgacg ccgagcagcc
     4081 cgatccgccg tcgcgccctg tcacgtcgga tccgattcct gcgtcgaaag ttcccgccgc
     4141 tgctgcgccc cctgtgatcg caccggtcaa agcgccgccg cgcgatcgca gcgcgccgat
     4201 cgtgatcccg gtcaagcacg acaggtcggc gaaagaggac aaggacgctg cgcccgaagc
     4261 gccgatcgcc gattcgatca gcgcggccgt cggcgcgatc gtgctacccg acgacgaaga
     4321 gcgcgctcgc gaagactcca agccgcactc gttcgacgat ttgaccgagg agcccgcggc
     4381 gatgcgcagc cgcgatatcc agatgccaac ggcggaagag ctcgcgcgca agaagccgcc
     4441 gacgccgccg tcgctcgcgg acatcgagga ggctcctgac gacgccggcg acttcgcgct
     4501 gacatcgccg ctgcgcgtgc tgttccgcct gatgacggcg cgtgcgaccg gtctgctcgt
     4561 cgtcgccgtc ggtggcatca agaaagagat ctacgttcgg gacggccagc cggagtacgt
     4621 gtcgtcgaac gtcgcgagcg agctgttcgg caactacctc gtgacgaagg gtgtgctctc
     4681 cgacggcgag ctggcgatgg cgctcgcgat gatgccgcac tacggcggca agctcggcga
     4741 cacgctcgtc gggctcggcc tgctcaagcc gctcgaggta ttccgtcacc tgacacgcca
     4801 ggtccgcacg aagatcatcg acgtgtgtac gtggaacaag ggcggcttcg cttggtacgc
     4861 cggccgcgag aacccgcgcg aagccttccc cctcgacttc aatgccttcg agatcctcgg
     4921 cgccggtgcg atggccctgg acccggacgt cgtcgacacc tggattgcca agagctcgag
     4981 tgtccgcctc cgcgcctccc gcgcgcgccg ggtcggtccc gagcgcttcg aggtcaaggg
     5041 tctcgtcgag cttttcgaga atctcgacgg aaaaacgacc attggcggcc tcgtcgacgc
     5101 ccagccggac cgcgaggagc ggcagcgcac cgcccggatg ctctgcctgc tcgaagcatg
     5161 cgacctcgcg cggcctgcgt gagtactgct agacttatcg tgtgaacgcc tcgactacgg
     5221 tcaaggccgg ggacacgctc gagggacggt acaagatcct taacctggtc ggtgaaggtg
     5281 gcatgggcaa ggtgtttctt gccgagcaca tgctcatcaa gcgccgggtc gctatcaaga
     5341 tcctccgccc cgagctggcc accgactcca acgtcgtcga gcgcttcatg aacgaagcgc
     5401 gcgcggcagg cgcgatcggt catccccaca tcgtcgagtc gaccgacatg gggttcacgc
     5461 gcgaagaggt gccgttcatc gtcttcgagt acctcgaagg ctcgctgctc accgacgaga
     5521 tctatcgcac caaaggcatg tcgccgcggc gcgcactgcg tatcgcaacc cagatcgcat
     5581 ccgcgctcca cgccgcgcac caagcgggca tcgttca
//
LOCUS       DATLBZ010000504         7496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5126955, whole genome shotgun sequence.
ACCESSION   DATLBZ010000504 DATLBZ010000000
VERSION     DATLBZ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5126955"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VMZ53_25420"
     CDS             <1..778
                     /locus_tag="VMZ53_25420"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31876.1"
                     /translation="FGGGGKDLDIGLTLYIMIEAAKGLNYAHQLENPQTGQKLNIVHR
                     DVSPPNIMMSKNGEVKVVDFGLAKAASQVEITDEGVVKGKFAYLSPEAALGQPVDART
                     DVFALGIITWEMLTGRRLFYADDPHATIKLVRQARVPSMSAYNSKVAPELEDIVRRAL
                     ARDPNDRTPNAADYAEELSNHLFDRELKVTSRDVANAVKAVRAAKKQPTASQNLINAL
                     IADEIAKMTSLVDKLPEASDAPKAVEVVDTSDWASELLDD"
     gene            complement(775..1350)
                     /locus_tag="VMZ53_25425"
     CDS             complement(775..1350)
                     /locus_tag="VMZ53_25425"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HUS31877.1"
                     /translation="MSNRLRDHSAIEARLLDLAHTTDAKLTAAALAYYAPCSIEDAQR
                     VLDDLTVKSVVAMDVEDDGTIVYTMPGRQKLGAPRAALPTPPTQPMGALIPFRPMHAP
                     VRYAVPRGASPLLAALLTLLVPGAGHLYAGRIITGIFWFLVVGLGYTLFFPGLILHLF
                     ATVSAANAAGRLEQQRQYVLAAQAQPYRLAA"
     gene            1453..2100
                     /locus_tag="VMZ53_25430"
     CDS             1453..2100
                     /locus_tag="VMZ53_25430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31878.1"
                     /translation="MKIAAVSFALVAVAACTGSEEPEAASPDAGITQPDAPPPEPKPA
                     FTASTMIGGKLAIDDAGIRATYKRGATEYKSRVTISLTDTATNKMCSVTLLPKLDSFG
                     HASTSTRQFKTVNLDFAGSTMMEDGCKWDDAWILSQLDQQFGHYIVGFAKARFAEDQP
                     YLDVYYDAVQPFPNSTANIVSAGSGTAYGMTAEGVVGLMMVEPAPGTLVPALYEF"
     gene            complement(2088..3098)
                     /locus_tag="VMZ53_25435"
     CDS             complement(2088..3098)
                     /locus_tag="VMZ53_25435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS31879.1"
                     /translation="MMARSSTPRSPTPRTIDDWLAHLKTLDLPRDADELRRVLALERP
                     GAGVAIAAVARRAAEHSLHAFAPELAAAFTRLVADAKRDPGCRGKNAIARCLHDLDVW
                     EEAVFAAGATLVQLEGPLSEITGKQDDMAAELRGICALAYARFLRPDALDVIADLLVD
                     EWAVTRIAAARALGACGRYDATALLRYKLLAVDDEPEVLAACCDSLLELSRDASPFLI
                     KLLSNHDDRAEAAALALGGARISDARDALIEWCTACSPDRRHRAGYVVLALLRDDVAN
                     AFLAEAIAKRAKADALAAARALATFNDHEPTRELLATAIASVKDTAVRRELGKLIADQ
                     NS"
     gene            complement(3095..3427)
                     /locus_tag="VMZ53_25440"
     CDS             complement(3095..3427)
                     /locus_tag="VMZ53_25440"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HUS31880.1"
                     /translation="MPITEAQIRKLALAYPEAIESSHFDVKDFRVRKKIFATIHPTGK
                     TGVLLHIDPDQRAALAADEPETFQLRGTALQVVFARITVAQYKALLEQAWRGTAPKKL
                     VAAYDAER"
     gene            3511..4560
                     /locus_tag="VMZ53_25445"
     CDS             3511..4560
                     /locus_tag="VMZ53_25445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31881.1"
                     /translation="MKRALVLALFVAACDPMGDFDIDLSGLEPGPIAGDRGAVTFALV
                     DGCPGGNFLFGCDKTMPAFAVGARARFVIGSASGSPSDEAKLAKATFVVSDPRVAAVG
                     RDAEGFVVLSALADGSAVIEVDDENGDVIDSISVDVERVVSLKGDMATTVLEGARLGA
                     SVIATGTSGRALFAHGAVVATLTGGIELDADPSGFFMSSEQVVIRVGKLLPPDTNSET
                     EPTESIARTRPAHIVWSTGAVSTDVSYTIVTRDDITSVKVSELWHEPSAYKQNLHADA
                     KVGEKYVRGGPACDWRIVSGGGDGAALASGVGDDTHSGFAFFDSALVYGNGDMTVECR
                     VNERVVGEMTVHLGP"
     gene            4646..6289
                     /locus_tag="VMZ53_25450"
     CDS             4646..6289
                     /locus_tag="VMZ53_25450"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HUS31882.1"
                     /translation="MRSCVFILILAACGHTTSGLGDDDDGVAPDSGPASRCVATPSAP
                     DDPSVVVTDGGELRGVERDTTRAFLGIPYAAPPIGDLRFRAPAQTACWQGVRDASTFG
                     QVCPQILPSGSKLGDEDCLFLNVFAPKAKSETKRPVMVFIHGGAYEVGSGNQDLVLDG
                     SGNLYDGTTLASEQDAIIVTLNYRLGALGFTAHPAFAIEDEHNSSGNYGTLDQIAALQ
                     WVRSNIARFGGDPDRVMIFGESAGALSVCLLLATPLAKGLFSSAIMESGGCRVAEKQS
                     RIQQGVELATRVGCDSDGDPAACLRSKGVFEIVTQVAADAGGMLGSDPATMWEMVHGP
                     NIDGYVFSEQPIKTIREGHHAKVPLVVGSNADEFAIFLPAITTCGGAYTFLYGVFGNR
                     TNEVVAQYPCDWTAPRDSVVAAMTDHMFTCEARRAARAAVAGGSPSVRRYFFTRHFTN
                     HALAALDAFHTAELPYVFRTFDVLGYTPNADDRQLSIDIGGYWGRVAASGDPNGGSAP
                     AWAAYSSTQDNALDLDMPLAMTQQVRAAKCDFWDTFVAN"
     gene            complement(6286..6783)
                     /locus_tag="VMZ53_25455"
     CDS             complement(6286..6783)
                     /locus_tag="VMZ53_25455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31883.1"
                     /translation="MGWKRAVRAAVALGLCGCVGPTIRDPHAGQRRLTFHNEAGGPVC
                     RLSIYPFGQSQPSNNFLEADTEIPSGGSVDYWVTPSTYQVHAEGCPYEKTQVGGYAPQ
                     VIMEKADAVTVLFREDDAKSKDAAQAIVHAHQNGTMIPAKLTVVGKPAARPTTVRPAR
                     TNARS"
     gene            6885..7145
                     /locus_tag="VMZ53_25460"
     CDS             6885..7145
                     /locus_tag="VMZ53_25460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31884.1"
                     /translation="MLAIASWKSDSVGSMKIRATLLLCAKVGVIAVAATGCGSRPNKI
                     MADTQVLPYQAPDISEITGIEEPDESDESEGEASAPAPEPGK"
     gene            7162..>7496
                     /locus_tag="VMZ53_25465"
     CDS             7162..>7496
                     /locus_tag="VMZ53_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HUS31885.1"
                     /translation="MCGIIGYIGNRQATPLLIGGLRKLEYRGYDSAGVSVYNDGMSRV
                     VRCRGKLGNLEEKLKAEPAVGTVGIGHTRWATHGRPSDENSHPHKVGPISVIHNGIIE
                     NHLQLRQELV"
BASE COUNT         1291 a         2415 c         2521 g         1269 t
ORIGIN      
        1 cttcggcggt ggcggcaaag atctcgatat cggcctgacg ctctacatca tgatcgaggc
       61 cgcgaagggc ctcaattacg cccaccagct cgagaatccg caaaccggac agaagctcaa
      121 catcgttcat cgcgacgtct caccgccgaa catcatgatg tcgaagaacg gcgaggtgaa
      181 ggtggtcgac ttcgggctcg cgaaagccgc cagccaggtc gagatcacgg acgagggtgt
      241 cgtaaagggc aagttcgcgt atctctcgcc cgaggcggcg ctcggtcagc cggtggacgc
      301 gcgcaccgac gtgttcgcgc tcggcatcat cacgtgggag atgctgacgg gccgccgctt
      361 gttctacgcc gacgatccgc acgcgacgat caagctggtg cgccaagcgc gcgtgccctc
      421 gatgtccgcg tacaactcga aggtcgcccc cgagctcgaa gacatcgtgc gtcgggcgct
      481 cgctcgcgat ccgaacgacc gcacgccgaa cgccgccgac tacgccgaag agctctcgaa
      541 ccacctgttc gatcgcgagc tcaaggtgac atcgcgcgat gtcgcgaacg cggtgaaggc
      601 cgtgcgcgcc gcgaagaagc agccgacggc gagccagaat ctcatcaatg cgctgatcgc
      661 ggacgagatc gcgaagatga cgtcgctcgt cgacaagctg cccgaggcgt cagacgcccc
      721 gaaggcggtc gaggtggtcg acacgtcgga ctgggcgtcc gagctcctcg acgactaagc
      781 ggctaaccga tatggctgcg cttgcgcggc gagcacgtac tggcgctgct gctcgaggcg
      841 gcctgcagca ttcgcggccg acaccgtcgc gaacaaatgc aggatgaggc ccgggaagaa
      901 cagcgtatag ccgaggccga cgacgaggaa ccagaagatg cccgtgatga tgcggcctgc
      961 gtagagatgg ccggcgcccg gcacgagcag cgtcaagagc gcagcgagca gcgggctcgc
     1021 accgcgtgga accgcgtagc gcacgggcgc gtgcatcggt cgaaacggga tgagcgcacc
     1081 catcggctgc gtcgggggcg tgggtagggc ggctcgcggc gcgcccagct tctgccggcc
     1141 cggcatcgta tagacgatgg ttccgtcgtc ctcgacgtcc atcgcgacaa cactcttcac
     1201 ggtcagatca tcgagcacgc gctgcgcgtc ttcgatcgag cacggcgcgt agtaagcgag
     1261 agccgctgcg gtcagcttcg cgtcggtggt atgtgcgaga tcgaggagcc gcgcttcgat
     1321 cgcgctgtga tccctgaggc ggttggacac gagaagcaac ataaccacgt ggaatccgtt
     1381 gtcactgcct gttcgggtag tgcgagcttc actgaaagtt cccatccaac cgccgcagcg
     1441 cgtctagcgg tcatgaagat cgccgccgtc tcgttcgctc tcgtcgctgt cgccgcctgc
     1501 acaggctccg aggaacccga agccgcaagc cccgacgcgg gcatcacgca acccgatgca
     1561 ccgccgccgg agcccaagcc cgcattcaca gcaagcacga tgatcggcgg caagctcgcg
     1621 atcgacgacg ccggcattcg cgcgacgtac aaacgcggcg cgacggaata caaatcgcgc
     1681 gtcaccatct cgctgaccga cacggccacg aacaagatgt gctcggtcac gctgcttccg
     1741 aagctcgact cgttcggtca cgccagcacg tcaacgcgcc agttcaagac cgtcaacctc
     1801 gacttcgccg gctcgacaat gatggaagac ggctgcaagt gggacgacgc gtggatcctg
     1861 agccagctcg atcaacagtt cggccactac atcgtcggct ttgcgaaggc tcgcttcgcg
     1921 gaagaccagc cgtacctcga cgtttactac gacgccgtgc agccgtttcc gaacagcacc
     1981 gcgaacatcg tgagcgccgg cagtggtacc gcgtacggaa tgacggccga aggcgtcgtc
     2041 ggtctcatga tggtcgagcc cgcgccgggc acgctcgttc ccgcgctcta cgagttctga
     2101 tccgcgatga gcttgccgag ctcgcgccgg accgcggtgt ccttcaccga tgcgatcgcg
     2161 gtggcgagca gctcccgtgt gggctcatga tcgttgaacg tcgcgagcgc gcgcgcagcg
     2221 gcaagagcgt cggccttcgc gcgcttcgcg atcgcctcgg cgaggaacgc gttggcgacg
     2281 tcatcgcgta gcaacgcgag cacgacatag cccgcgcgat gccgacggtc cggcgagcac
     2341 gcagtgcacc attcgatcag cgcatcgcgt gcgtcactga tacgggcgcc gccaagtgcg
     2401 agcgccgccg cttcagcgcg gtcgtcgtgg ttggagagga gcttgatgag gaatggcgat
     2461 gcgtcacgcg agagctcgag caacgagtcg caacatgcgg caagtacttc gggctcgtcg
     2521 tcgaccgcga gcagcttgta gcgaagcagc gcagtcgcgt cgtaccggcc acaggccccg
     2581 agcgcgcgcg ctgccgcaat tcgggtgacg gcccattcgt cgactagaag gtcggcgatc
     2641 acgtcgagcg cgtccggacg caagaagcgc gcgtaagcga gcgcgcaaat gccccgcagc
     2701 tctgcggcca tgtcgtcttg cttgccggtg atctcggaga gcgggccctc gagctggacg
     2761 agcgtcgcgc cggccgcgaa gaccgcttcc tcccacacat cgaggtcgtg gaggcagcgc
     2821 gcgatcgcgt tcttgccccg gcagcctggg tcacgcttgg cgtcggcgac aaggcgcgtg
     2881 aacgccgcag cgagctccgg tgcgaacgca tgcaggctgt gctccgcggc tcgacgcgca
     2941 accgctgcga tcgccacgcc cgcacctggg cgttcgagag cgagcacgcg gcggagctcg
     3001 tcggcatcgc ggggtaagtc gagcgttttc aagtgcgcga gccagtcgtc gatggtacga
     3061 ggcgtcgggc tgcgcggcgt cgagcttcgc gccatcaccg ctccgcgtcg tatgcagcga
     3121 cgagcttctt cggcgcggtg ccgcgccatg cttgctcgag caacgccttg tactgcgcga
     3181 cggtgatgcg agcgaaaacg acttgcagcg cggtgccccg cagctgaaac gtttcgggct
     3241 cgtcggcggc gagcgcggcg cgctgatccg gatcgatgtg gagcagcacg ccggtcttgc
     3301 ccgtcggatg gatcgtcgcg aagatctttt tgcgcacgcg aaagtctttg acgtcgaagt
     3361 gcgacgactc gatcgcctcg ggataggcga gcgcgagctt gcggatctgc gcttcggtga
     3421 tcggcatgcg ctggcatctt cgcaggcttt ccgttcgctg ccacttgctg cgctacgcag
     3481 gtcggaccac tcgcgggtat tccgaccatg atgaaacgcg ctcttgttct cgctctcttc
     3541 gtggccgcct gcgatccgat gggcgatttc gatatcgatc tgtcggggct cgagcctgga
     3601 cctatcgccg gtgatcgcgg agcggtcacg tttgcgctcg tcgatggctg tcccggcggg
     3661 aacttcctgt tcggctgcga caagacgatg cccgcgtttg ccgtcggagc gcgagcgcgc
     3721 ttcgtgatcg ggtcggcgag tggcagccct tcggacgagg ccaagctcgc gaaggcgacg
     3781 ttcgtcgtga gcgatccgcg tgtcgccgcg gtagggcgcg atgccgaagg cttcgtcgtc
     3841 ttgtcggcgt tggcagacgg cagcgcggtg atcgaagtcg atgacgagaa tggcgatgtg
     3901 atcgattcga tcagcgtgga tgtcgagcgc gtcgtatcgc tgaagggcga tatggcgacg
     3961 acggtcttgg aaggtgcgcg gcttggcgcg tcggtcatcg cgaccggaac atcggggcga
     4021 gcactcttcg cgcacggcgc tgtcgtcgcg acgctcacgg gtggcatcga gctcgatgca
     4081 gacccgagcg gcttttttat gagcagcgag caggtcgtga ttcgcgtcgg caagctcttg
     4141 ccgcccgata cgaacagcga aaccgagccc accgagagca tcgcgcgcac gcggcccgca
     4201 cacatcgtgt ggtccacggg cgcggtgtcg accgacgtga gctacacgat cgtcacgcgc
     4261 gacgacatca cgtcggtgaa ggtctcggag ctgtggcacg agccctctgc gtacaagcag
     4321 aacctccacg ccgatgcgaa ggtcggcgag aagtatgttc gcggtgggcc ggcgtgtgat
     4381 tggcggatcg tgtcgggcgg cggcgatggc gcagcgctcg cgtcgggcgt cggcgacgac
     4441 acgcatagcg gcttcgcgtt cttcgacagc gctctcgtct atggcaatgg cgacatgacc
     4501 gtcgagtgcc gcgtgaacga gcgcgtcgtc ggcgagatga ccgtccacct cggaccgtga
     4561 gtgagacgtg ctcgctgtta cacccgtgta atggcggccc gtggtatgcc gccctcgatg
     4621 atgaggagcg cctgagaggc actcgatgcg gtcttgcgtt ttcatcctca ttcttgcggc
     4681 ttgcggccat acgacgagcg gtctcggcga cgacgacgac ggcgtagcgc ccgatagcgg
     4741 tcccgcctcg cgatgcgtcg cgacgccgag cgcgccagat gacccgagcg tcgtcgtcac
     4801 cgacggtggc gagctgcgcg gcgtcgagcg cgacaccacc cgcgcattcc tcggcattcc
     4861 gtatgcggcc ccgccgatcg gcgacctccg cttccgggca ccggcgcaaa ctgcgtgctg
     4921 gcaaggcgtg cgcgacgcgt cgacgttcgg ccaggtctgc ccgcagatcc tgccgtcggg
     4981 cagcaagctc ggcgacgagg actgcctgtt cttgaacgtg ttcgcgccca aggcgaagag
     5041 cgagacgaag cggccggtga tggtcttcat tcacggcggc gcgtacgagg tcggctcggg
     5101 caatcaagac ctcgtcctcg acggcagcgg caacctctac gacggcacga cgctcgcgag
     5161 cgagcaggac gccatcatcg tgacgctcaa ctaccggctc ggcgcgctcg gcttcaccgc
     5221 gcatcccgcg ttcgcgatcg aggacgagca caactcgagc ggcaactacg gcacgctcga
     5281 tcagatcgcg gcgctgcagt gggtgcggag caacatcgcg cgcttcggcg gcgatccgga
     5341 ccgcgtgatg atcttcggcg agtcggcggg cgcgctctcg gtgtgcctac tcctcgcgac
     5401 gccgctagcg aagggcctct tctcgtcggc gatcatggag agcggcggct gccgcgtcgc
     5461 cgagaagcag tcgcggatcc agcagggcgt cgagctcgcg acgcgcgtcg gttgcgacag
     5521 cgacggtgat ccagcggcgt gcctgcgcag caagggcgtg ttcgagatcg tcacgcaggt
     5581 ggcggcggat gccggcggca tgctcggcag cgaccccgca acgatgtggg agatggtcca
     5641 cgggccaaac atcgacggct acgtattcag cgagcagccg atcaagacga tccgcgaagg
     5701 gcatcacgcg aaggtgccgc tcgtcgtcgg cagcaatgca gacgagttcg ccatcttctt
     5761 gcccgcgatc acgacgtgcg gcggcgcgta cacgttcctc tatggcgtgt tcggcaatcg
     5821 cacgaacgag gtcgtcgcgc agtatccgtg cgactggacg gctcctcgcg acagcgtcgt
     5881 cgcagcgatg accgatcaca tgttcacgtg cgaagcgcgt cgcgcggcac gtgctgcggt
     5941 cgcgggcggg tctccgagcg tgcgtcgcta tttcttcacg cgtcacttca cgaatcacgc
     6001 gctcgccgcg ctcgacgcct ttcacacggc cgagctgccg tacgtgttcc ggacgttcga
     6061 cgtgctcggc tacacgccca acgcagacga ccgccagctc tcgatcgaca tcggcggcta
     6121 ctggggccgc gtcgcggcga gcggcgatcc gaatggcggc agcgcgccgg cgtgggcagc
     6181 gtattcatcg acgcaggaca acgcgctcga cctcgatatg ccgctcgcga tgacccagca
     6241 agtgcgcgcc gccaagtgcg acttctggga cacgttcgtc gcgaactaac tacgcgcgtt
     6301 cgtgcgtgcg ggccgcaccg tcgtgggcct tgccgccggt ttgccgacga ctgtcagctt
     6361 cgcgggaatc atcgtgccgt tctgatgcgc gtggacgatc gcctgcgcgg cgtctttgct
     6421 cttcgcgtcg tcttcgcgaa acagcacggt gaccgcgtcg gccttctcca tgatcacctg
     6481 cggtgcgtag ccgccgacct gcgtcttctc gtacgggcag ccctcggcgt ggacctggta
     6541 tgtgctgggc gtcacccagt agtcgaccga gccgccgctc gggatctcgg tgtcggcctc
     6601 gaggaagttg ttgctcggct gcgactggcc gaacggatag atcgagaggc ggcagacggg
     6661 acctccggcc tcgttgtgga acgtcagtct ccgctggccg gcatgagggt cgcgtatcgt
     6721 cggtccgacg cagccgcaca gtccgagcgc gacggccgcg cgcactgcgc gtttccaccc
     6781 catgtgttca ctatcgcaca gggttaatcg cggtcgatgc cgcgataacc acgaggttcg
     6841 aatagcttcc ggccgcggcg cgtttcatgc acaagggtcc gcgggtgctc gccatcgcga
     6901 gttggaaaag tgatagcgtc ggctcgatga agattcgagc gactctcttg ctttgcgcga
     6961 aggttggcgt gatcgcggtt gcggccacgg gttgcggttc gcgtcccaac aagatcatgg
     7021 cggacaccca ggtgcttcct tatcaggcgc ctgacatcag cgagatcacc ggtatcgaag
     7081 agccggatga gtcggacgaa tccgagggcg aagcctcggc gccggctccc gagccaggca
     7141 agtaacgctc aggaggtttt catgtgcggc atcatcggtt acatcggcaa tcgccaagca
     7201 accccgttgc tcatcggcgg actgcgtaag ctcgagtatc gcggttacga ctcggctggt
     7261 gtctccgtct acaacgacgg catgtcgcgt gtcgtccggt gtcgcggcaa gctcggaaac
     7321 ctcgaagaga agctgaaggc ggagcccgct gtcggcaccg tcggcatcgg ccacacgcgc
     7381 tgggccacgc acggccgtcc ctctgacgag aacagccacc cgcacaaggt cggcccgatc
     7441 tcggtcattc acaacggcat catcgagaac cacctgcagc tgcgccaaga gctggt
//
LOCUS       DATLBZ010000505        12893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5128062, whole genome shotgun sequence.
ACCESSION   DATLBZ010000505 DATLBZ010000000
VERSION     DATLBZ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12893
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5128062"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VMZ53_25470"
     CDS             <1..869
                     /locus_tag="VMZ53_25470"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUS31886.1"
                     /translation="VQERSFSAAARDLGLVKSAVSKRISLLEERLGVRLLTRTTRKLS
                     LTEDGARFYEHCAALLAAADAAEEAVAGASSEPRGRLLVNAPVSFAQMYLTDALAGFL
                     RKHPSIEVDLSADDRMVDVVEGGFDVVVRITRLADSSMVARRLSMDRLVVCASPDYLA
                     RHGRPETPAELVGHNCLHYSLVSQAGEWRFRDAQKQPFVVPIAGNFRTTDGATLWRAA
                     LAGVGIAVTPLFMVARDVQAGRLELLLEGARKAEIGIYAMFASRRQLPARTKLLVEHL
                     VGWFAEPAWRLR"
     gene            complement(933..1214)
                     /locus_tag="VMZ53_25475"
     CDS             complement(933..1214)
                     /locus_tag="VMZ53_25475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31887.1"
                     /translation="MTDLATISMDDLSVVCGGQQGAPQQPQQPAPNPAADYSTNGNVV
                     QQGAQMIDNAYGAFQGARKAGASWYESLGNAAIGAFNLGGGFDRNGRPR"
     gene            complement(1411..5616)
                     /locus_tag="VMZ53_25480"
     CDS             complement(1411..5616)
                     /locus_tag="VMZ53_25480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31888.1"
                     /translation="MPALPKPHRVAFLVPDVKIDGPDAAYEREAAVLIWTACIETCQR
                     HPRLAVLDADATPLFPQDGHFAPLHADRGGRATDAFFGPTRRDEVLWLELRLGSKPSV
                     VRLHAVGRDGKQESYDALGPKLGEQIQQVFGAWLTARKLGTLPRRFEDITTDHVLAAL
                     RVIAPTLAEQARAWSLPVATKPTWSLSIVEDEPGDDEEPEPERDENPEDPDEEFDAAF
                     EAAIDSTIDSTQDLEPSAPGTPPEAPRRRSLSRPLVARLQPPFKLPALRLLELALRED
                     LSEAILVVDPAHPQALFAQFEKTAPHSKDFVLLRKIIAAAPGWARPYEELRVAEDSDD
                     DDNAQAPSDLEAVAAAGIATLSRPGGLQGLETVGDLLSEDGRVDEGIRLLERGVELHM
                     EDPGAHLALLSLHDDTDREGARLEQAFRSGSLHGCPMDPVLPWYPDQIHIDLRVATAL
                     LAVGRLDEAIALRANRLEGREAQWPHHTRILASWRKDPRFVAWSYAREGYFRGDDARG
                     VEGFSRIEPDDNVDLAIFLDSLVALGREDEVPLAWAQYGLGHGYDGPIARIVAARALM
                     AAGEYRRGIEEMWRVELTEPGRDEHVAVARCGLIMAAAPIDVIEAALGERVAIGAPTL
                     AKKMARDVADFVPAAAKSGVVARALGLGPKAQTIDFDMEWLAGFSPDTRSRRAIDQMF
                     ADLGSFRKGPPQGFDISDELKKGDRIVNRWLEVAFAEASEEDPAALAQAAAYLAAQAL
                     GRYLAATSYPPTTLAGALRAVAGGALALVRKHRRSLGDREARAVLATIEPLLRRVDRW
                     VGSTWLATVERSLGIDERSGGDVLGFARDFPSVSGRILGPEETAVLSWSVARLHRDRP
                     DGWAAKIVVQAARLAAHTGYAGCDEWADAVVAQLAAREIEQDDAIDALHTACYLAQGV
                     SAIPCVHAARVLFDAGRAPAALAVLSAGLEAAGEEWRDKALASLSDAWKRSKIDVPIE
                     FEKVAAGVFEALQKGDPLRGEKLARWAVAYDPSNEEAHRNLGLALAMQGKTIDALAHL
                     VKSTPEQATQILSGVLYQNGKLPESMAVLDYASRWYVRADQWLTYGGIAYAAMDNPRT
                     VKAYALAYQLDPDAFDASQLNAYAGVLDEVGDYAMCEKVANHLIRVAGKDKMWLTNGW
                     NHLACAYIGQDKFEEAIELANRAVKENPLPDNAGNFAATLQRAFTKQKTAPTAPAEQK
                     KRERVFELMEAGDLAAAAEMITDKSWRVRRAALHAARFRFASENQVEVTPRARAAALA
                     ILDDTVGTMDKDALLARSVALQIREQAYFARDPAPSLGDRMTRDAFYAEFRARGGVVL
                     GEDAPPPPAFVDRVVVPGSRIERASDYIALLRDLANLAPREALAQFELDEAMYIEVAT
                     AWANAMEKDPTIAKTIATGLAKR"
     gene            complement(5616..7400)
                     /locus_tag="VMZ53_25485"
     CDS             complement(5616..7400)
                     /locus_tag="VMZ53_25485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31889.1"
                     /translation="MVDLPVQLGAMDAVPPAVRYRIRDVIGLLQGGPLEGVASERLNR
                     LLDEGEPWIGADPSLAEVGYWMLQAKHTPEMRRQGCVWLTMFPTVETMTRVAAVALDS
                     ATPVVVREQAIWTLGYRQVRCMHPSTRWSPEAVQLADETLIKLGDAATSAGKVASEQL
                     PLAMRHIQWEGASAIFARAPGLWGEAIESFASPAFARVLLVCLEDIPPQHRVRAIRLV
                     GAVLGDECIPMMLAKAGQASIDEKLEMLFMVISLAGEAKLGVLEDAIKGMKFVDLLRQ
                     RAKWHLQNPRVVPTVRGLRVARSTALMAPEGRQKACAQAADDLGVLTKFARHPEAYLY
                     TMWGWMVRGSCDPARARDLVAAHPESQRLVRDLYMEDLAKRGRVKLLSAAAQTLQGAD
                     FGALHLATFGRPFAALELAATARLHTPELVCARVLACYRAGRPDLAKRVLAEDLPPSE
                     IVDDTALPPFPGPHEKWLIEHAPTSRPAIGALAGGLDAVVALAKAAPLEAEPDSTSLE
                     PIQAVIRRLGRGLPGSTVYLAGEFKYLDKGKIAKAVEAAGARLVNGPFPGTDYYVHGD
                     WCLVQTIAQLERQGARRLRRGELPGADV"
     gene            7443..8129
                     /locus_tag="VMZ53_25490"
     CDS             7443..8129
                     /locus_tag="VMZ53_25490"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2012 domain-containing protein"
                     /protein_id="HUS31890.1"
                     /translation="MSRFALALLLVSSTATAGSIKGTVLFEGEPPEQQTLQRDSDPKC
                     SKTKADEAVVVTKGKLRDVLVRIKNGSGGKHDAPAATVLIDQKDCMYSPRVVGIVAGQ
                     KLQVRNSDNTFHNVWGQVSNKDVFNKPHAAKAADLTLDPSAAKADDIVELKCGVHGWM
                     HGYIAVQDHPYFAVTASDGSFVIKDLPQGSYTLEAWHPELGTKTMKVVIGKGKRADVT
                     ARFSYKQSER"
     gene            8284..10071
                     /locus_tag="VMZ53_25495"
     CDS             8284..10071
                     /locus_tag="VMZ53_25495"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HUS31891.1"
                     /translation="MEPTEDATEPQGVGLQGALVKMEMSSDVAVLLDEIPAGPMREAA
                     VANVMAKPASFWTERAARQVRLTYYRLVFRGLYYSSDWGNASKVYGPLPLPDKSVWNV
                     QLTGTPHRVSAINGQNHDSIVVSYKFDTMIVTDKDSPGKVDPSLATLGGTADEVFTLP
                     TDPDLLLQRTGYACMDEEEYPPGSVFEENTWYFYDDTCKAGNSWCHITEQPGESCINA
                     VNKRVGDVKTTMHFTRVPYDATLASKYRVGAITNRDGADLAVMQKGMEEEKKIAYRFF
                     APGSCELEEGVIAKLGWRRLLMFSAIVQNNGTQPVHIGDVTDSNNPWVRSGAFEFSAC
                     HGHYHFSHYGTFNYNGAPGSKRAFCLEDTNRFHNDETTPLTADHQSCHYQGIGAGWGD
                     EYQFGLPGQWVDITDVDTRKAHPLTFDSNPDMFLCEGKPALDSNGQLILDPSPFKDAQ
                     GNTVSRVRCNMPTTWHDNNVGTVSVSSPGGSFVTESCKLGEIGHDRDCGYAAQTQLRS
                     CQAGSTVTLTCKNTGAPQALRICEKSGALGVGVACTFRDSVANPTINGSTTFSFTCPA
                     VRDYPGAGGYSLYNASVVPSMGTGSVTCN"
     gene            complement(10072..10854)
                     /locus_tag="VMZ53_25500"
     CDS             complement(10072..10854)
                     /locus_tag="VMZ53_25500"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HUS31892.1"
                     /translation="MDPWSVLTEHQAPRARAFLADRERERKHLVVYLSGAHAYGFPSP
                     DSDLDLKCVHIASTRALVGLNPPDDPPGRIDIVDGVELDYGSNELGGVLRGILKGNGN
                     YIERIFGELVLGGDLVRVESARAVLAPVISRRVGKHYGGFAMSQLRAFDEKPTAKRAL
                     YVLRTAATGLHLLLTGNVVTDVAKLAEHLPAEISELMAIKQRGEKQPLEADHARAWRS
                     RLAAAIDAIDAGWPRSVLPAEPPPEAIAAVDEWLRDVRQQSW"
     gene            10879..11373
                     /locus_tag="VMZ53_25505"
     CDS             10879..11373
                     /locus_tag="VMZ53_25505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31893.1"
                     /translation="MRVLALLALAACSNVAVRVRPADLSVHAQEIVNTGEADVPFEGT
                     TVHVKASERVEVRLADGDTQRLATLTIGELVQGCVPDPDSKQCLANQAVRDPFVVRRE
                     HHVRSGSLETGVAFGLMGGAVGTCLAVCQDRAELGEGLAYVGLGVAAFMGLFLLVVMA
                     GGRD"
     gene            complement(11370..12260)
                     /locus_tag="VMZ53_25510"
     CDS             complement(11370..12260)
                     /locus_tag="VMZ53_25510"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HUS31894.1"
                     /translation="MSEARTAPDIVILSPHLDDAVLSVGGLISREVAAGKRVEIVSCF
                     TAGPPLETIAPERRVFGDYSMRRAEDERALATLGATHRWLDLHERIWRDPPLPRTRGV
                     FPTYVFRTPERMDDFRELKAIRAVITDILERGTRTIYAPLAIGHHVDHAEVALAALRE
                     VLGSGAFERIRFYEDVYGLGRACRKQHFVAKRRMWRWFGAPAWASPRVGALLRLVAMA
                     RKGPGLEEYLPEAARLEWTCAPQEVAKLDEERKLAAISEYGSQVRAFGGMARVTAFVR
                     RAHKIMGGELTWTCRPASPR"
     gene            complement(12253..>12893)
                     /locus_tag="VMZ53_25515"
     CDS             complement(12253..>12893)
                     /locus_tag="VMZ53_25515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31895.1"
                     /translation="LAEHRTTVIGWLRDDPSLELDAAAERLADTARVHAPTRHDAINR
                     ARDYIKQLYRSMYDQGLIAANDWLSLRAAAKTEPELERGGPAPPSGIALPRDLRPKNA
                     YNLIRLLDLAIRWLSAEPPTVRVSDSLRPTLIAIEKGEVPMPDVMALARELTPKLEAA
                     RQTSPLPRYPDVARADRVLRAARAEAARRCVQCTDDAWGAHSAAPPEARFDE"
BASE COUNT         2133 a         4361 c         4279 g         2120 t
ORIGIN      
        1 tcgtccagga gcgctcgttc agcgccgccg cgcgcgacct cggcctggtg aagtccgctg
       61 tgagcaagcg catctcgctc ctcgaagagc ggctcggcgt acgcctcctc acgcgtacaa
      121 cacgcaagct gtcgctgacc gaggatggcg cgcggttcta cgagcactgt gcagcgctgc
      181 tcgcggccgc cgatgcggcg gaggaagcgg tggcgggcgc gagctcggag ccgcgagggc
      241 ggctgctcgt caacgcgccc gtgtcgttcg cgcagatgta cttgaccgac gcgctcgctg
      301 gcttcttgcg caagcacccc agcatcgagg tcgacctcag cgccgacgac cgaatggtcg
      361 acgtcgtcga gggaggcttc gacgtggtcg ttcggatcac gcggcttgcg gattcgtcga
      421 tggtggcgcg ccggctgtcg atggatcgcc tcgtcgtgtg cgcttcgccc gactatctag
      481 cgcgccacgg ccgtcccgaa acgcccgcgg agctcgtcgg ccacaactgc ctgcactact
      541 cgctcgtgtc gcaagccggc gagtggcggt ttcgcgacgc gcagaagcag ccgttcgtcg
      601 tgccgattgc cggcaacttt cgaacgaccg atggcgccac gctctggcga gccgcgctgg
      661 ccggcgtagg tatcgcggtc acgccgctgt tcatggtcgc gcgcgacgtc caggctggcc
      721 ggctcgagct gttgctcgaa ggcgcacgca aagccgaaat cggcatctat gcgatgttcg
      781 cgtcacgtcg acaattaccc gcgcgcacga agctcctcgt cgagcatctc gtgggctggt
      841 tcgccgagcc cgcctggcgc ttgcgctagc ggtttgcgtg gcccgccgac gtcccctcga
      901 acgtctgacg ggcacacgcg cgtagctaac actcaacgcg ggcgaccgtt gcggtcgaag
      961 ccgccgccga ggttgaacgc accgatcgcc gcgttgccga gcgactcgta ccagctcgcg
     1021 ccggctttgc gcgcgccttg gaacgcgccg tacgcgttgt cgatcatctg cgcgccctgc
     1081 tggaccacgt tgccgttcgt cgagtagtca gcggccgggt tcggcgcggg ctgctgcggc
     1141 tgctgcggcg cgccctgctg tccaccacac accaccgaga ggtcatccat cgagatcgtt
     1201 gcgaggtccg tcatcacact ttgctcctgc tgctttgttt gttcgtcgtc gagacatttc
     1261 gacgatgaca actccatgtg cagcgggtgt gccgagtcgt gcacgttgtt gcatcgcgag
     1321 gttgcgacgc tgcgcaacgc ggcgactgtc aaatgcggtg agcgaactgc gatctcggac
     1381 gcgcgacgcc gacggtagac agccgtcaac tcagcgtttt gccaggcctg tcgcgattgt
     1441 cttggcgatc gtcgggtcct tctccatcgc gttcgcccat gcggtggcga cctcgatata
     1501 catcgcctcg tcgagctcga actgcgcgag cgcttcgcga ggcgcgaggt tcgcgaggtc
     1561 acgcaggagc gcgatgtagt cgctcgcgcg ctcgatccgg gagcccggca cgacgacgcg
     1621 atcgacgaat gctggaggcg gcggcgcgtc ttcaccgagc acgacaccac cgcgtgcgcg
     1681 gaactccgcg tagaacgcgt cgcgcgtcat gcggtcgcca aggctcggcg ccggatcgcg
     1741 cgcgaagtac gcctgctcgc gaatctgcag cgcgaccgac cgcgcgagga gcgcatcctt
     1801 gtccatcgtg ccgacggtgt cgtcgagaat ggcgagtgcg gcggcacgag cgcgcggcgt
     1861 gacctcgact tgattctccg acgcgaagcg aaaacgagct gcgtgcagcg cagcgcggcg
     1921 gacacgccac gacttatccg taatcatctc ggcggcggcg gcgaggtcgc ctgcctccat
     1981 cagctcgaat acgcgctcgc gtttcttctg ctcggccggc gccgtcggtg cggtcttctg
     2041 cttcgtgaat gcgcgctgca gcgtcgctgc gaagttgccg gcgttatcgg gcagcggatt
     2101 ctctttgacg gcgcggttcg cgagctcgat cgcctcttcg aacttgtcct gaccgatgta
     2161 cgcacacgcc aggtgattcc acccgttcgt cagccacatc ttgtctttac ccgcgacgcg
     2221 aatgaggtgg ttcgcgacct tctcgcacat cgcgtagtcg ccgacctcgt cgaggacgcc
     2281 cgcgtacgcg ttgagctgcg aagcatcgaa tgcgtccggg tcgagctgat acgcgagtgc
     2341 gtatgctttg accgtgcgcg ggttgtccat cgccgcgtac gcgatgccgc cgtacgtgag
     2401 ccattgatcg gcgcgcacgt accaccggct ggcgtagtcg aggacggcca tcgattcggg
     2461 cagcttgccg ttctgataga ggacgcccga caaaatctgc gtcgcttgct ccggcgtgct
     2521 cttcacgaga tgagcgagcg cgtcgatggt cttgccctgc atcgcgagcg cgagcccgag
     2581 gttgcgatgt gcttcctcgt tcgacggatc gtaggcgacg gcccaccgcg cgagcttctc
     2641 gccacggagc gggtcgccct tctgcagcgc ttcgaacaca cccgccgcga ccttctcgaa
     2701 ctcgatcggt acgtcgatct tgctgcgctt ccatgcgtcg gacagcgacg cgagcgcttt
     2761 gtcgcgccac tcctcgcctg ccgcttcgag gcctgcggac agtacagcga gcgcggccgg
     2821 tgcacgacct gcgtcgaaca ggacgcgcgc agcgtgcacg catggaatcg cgctgacgcc
     2881 ctgcgcgaga tagcaggcgg tgtgcagcgc atcgatcgcg tcgtcttgtt cgatctcgcg
     2941 agcggcgagc tgtgcgacca cagcatcggc ccactcgtcg caacctgcgt agcccgtgtg
     3001 cgcggcgagg cgcgccgcct ggacgacgat cttcgccgcc cagccatccg ggcgatcgcg
     3061 atgcagacgt gcgaccgacc acgacagcac ggcggtctcc tcgggaccga ggatgcgacc
     3121 ggacaccgac ggaaagtcgc gcgcgaagcc gaggacgtcg ccaccactgc gctcgtcgat
     3181 gccgaggctg cgctcgacgg tcgcgagcca cgtgctgccg acccagcggt cgacgcgacg
     3241 cagcagcggc tcgatggtcg cgaggaccgc gcgagcttcg cgatcgccga gcgaacggcg
     3301 atgtttgcga acaagtgcga gggcaccgcc tgcaacggcg cgcagcgcgc ctgcgagcgt
     3361 cgttggtggg tagctcgtcg ctgcgaggta tcggccgagc gcttgcgcag cgagatacgc
     3421 agccgcttgc gcgagtgcgg cgggatcctc ctcggaggct tcggcgaatg cgacctcgag
     3481 ccagcgattg acgatccggt cgcctttctt cagctcgtcg gagatgtcga agccttgcgg
     3541 cgggcctttc cggaaggaac cgaggtctgc aaacatctgg tcgatcgcgc ggcggctgcg
     3601 cgtatcgggc gagaaccccg ccagccactc catgtcgaag tcgatcgtct gcgcctttgg
     3661 accgaggccc agcgcacgcg ccacgacacc gctctttgcg gcagccggaa cgaagtcggc
     3721 gacgtcgcgc gccatcttct tcgccagcgt cggtgcaccg atcgcgacgc gctcgccgag
     3781 cgctgcctcg atcacatcga tcggtgccgc ggccatgatc aggccgcatc gcgctactgc
     3841 gacgtgctcg tcgcgacccg gctcggtgag ctcgacgcgc cacatctcct cgatcccacg
     3901 ccggtactcg cccgcggcca tcagcgcgcg cgccgcgacg atccgagcga tcggcccgtc
     3961 gtagccatgg ccgaggccgt attgcgccca cgcgagcggc acctcgtctt cgcggccgag
     4021 tgcgacgagc gaatcgagga agatcgcgag atcgacgttg tcgtccggct cgatgcgaga
     4081 gaacccctcg acgccgcgcg catcgtcgcc gcggaagtag ccttcacgcg cgtagctcca
     4141 cgcgacgaag cgaggatcct tacgccagct cgcgaggatg cgcgtgtgat gcggccactg
     4201 cgcttcgcgg ccctcgaggc ggttcgcccg caacgcgatc gcttcgtcga gacggccgac
     4261 cgcgagcagc gccgtcgcaa cgcgcagatc gatgtggatt tgatcgggat accaggggag
     4321 caccggatcc atcggacagc cgtgcaggct tcccgaccga aacgcttgct cgaggcgcgc
     4381 gccctcgcga tcggtatcgt catgcaacga gagcagcgcg agatgcgcgc ccggatcttc
     4441 catgtgcaac tcgacgcccc gctcgagcag gcgaatgccc tcgtcgacgc gaccatcttc
     4501 ggagagcaag tcgccgacgg tctcgaggcc ttgcaggccg cccggccgtg agagcgtcgc
     4561 gatgcccgcc gcagcgaccg cttcgagatc gctaggcgct tgtgcgttgt cgtcgtcgtc
     4621 gctgtcttcc gcgacgcgca gctcttcata cggccgtgcc cagcccggcg ccgccgcgat
     4681 gatcttgcga aggagcacga agtccttgct gtgcggcgcg gtcttctcga actgcgcgaa
     4741 cagcgcctgc gggtgcgctg gatcgacgac gaggattgcc tcggagaggt cctcgcggag
     4801 ggcgagctcg agcagacgca gcgcgggcag cttgaacggc ggctgcaagc gagctacgag
     4861 cgggcgcgag agcgagcgac ggcgaggagc ttcgggaggc gtgcccggcg cggatggctc
     4921 gagatcctgt gtcgagtcga tcgtcgaatc gatcgctgcc tcgaacgcgg catcgaattc
     4981 ctcgtccgga tcctcgggat tctcgtcgcg ctccggttcg ggctcttcgt cgtcacccgg
     5041 ctcgtcctcg acgatcgaca gactccacgt cggcttggtc gcgacgggca gcgaccacgc
     5101 gcgtgcttgc tcggcgagcg tcggtgcgat cacgcgcagc gcggcgagca cgtgatccgt
     5161 ggtgatgtcc tcgaaccgac gcggcagcgt gccgagcttg cgcgcggtga gccacgcgcc
     5221 gaacacctgc tggatctgct cgccgagctt ggggccgagc gcgtcgtacg actcctgctt
     5281 gccatcacgg ccaacggcat gaagccgaac gacgctcggt tttgatccga gccgcagctc
     5341 gagccagagc acctcatcgc gccgggtcgg gccgaagaac gcgtcggtgg cgcggccgcc
     5401 tcgatccgca tgcagcggcg cgaagtgacc gtcctgcgga aacagcggcg ttgcatcggc
     5461 gtcgaggacg gcgaggcgcg gatggcgctg gcaggtctcg atgcacgccg tccagatcag
     5521 caccgcggct tcgcgctcgt acgcggcgtc ggggccgtcg atcttgacgt cggggacgag
     5581 gaaggcgacg cgatgcggtt tcggtagcgc cggcattaca cgtctgctcc cggcagctca
     5641 ccacggcgca acctgcgcgc gccttgtcgc tcgagctgcg cgatcgtctg cacgaggcac
     5701 caatcgccgt gcacgtagta gtcggtgccg gggaacggcc cgttgacgag ccgcgcaccc
     5761 gccgcttcaa cggcctttgc gatcttgccc ttgtcgagat acttgaactc gccagcgaga
     5821 tacaccgtcg agcccggcaa gccgcggccg aggcgacgaa tcaccgcttg gatcggctcg
     5881 agcgacgtcg agtccggctc tgcttcgagc ggcgctgcct tcgcgagcgc gacgacggca
     5941 tcgaggccac ccgcgagcgc accgatcgcc ggccgcgacg tcggcgcatg ctcgatgagc
     6001 cacttctcgt gcggtccggg gaacggcggc aaggccgtgt cgtcgacgat ctccgacggt
     6061 ggcaggtcct cggcaagcac gcgcttcgcg agatcgggac ggcccgcgcg gtagcacgcg
     6121 agcacgcgag cgcacacgag ctcgggcgta tgcaggcgcg cggtggcagc gagctcgagc
     6181 gcagcgaatg ggcggccgaa cgtcgcgagg tgcagcgcgc cgaagtctgc tccctgcagc
     6241 gtttgcgcag ccgccgacag cagcttcacg cgaccgcgct tcgcgagatc ctccatgtag
     6301 agatcgcgga cgaggcgctg cgattcggga tgcgccgcga cgaggtcgcg tgcacgcgct
     6361 ggatcgcacg agccgcgcac catccagccc cacatcgtat agaggtacgc ctcggggtga
     6421 cgcgcaaact tcgtgagcac gccgaggtcg tcggccgctt gcgcgcatgc cttctgccgc
     6481 ccctctggcg ccatcagcgc ggtcgagcgc gcaacacgta ggcctcgcac cgtcggcacg
     6541 acgcgcggat tctggagatg ccacttcgcg cgctgccgca gcaggtcgac gaacttcatc
     6601 cccttgatcg catcctcgag cacgccgagc ttcgcttcac ccgcgaggct gatcaccatg
     6661 aacagcatct cgagcttctc gtcgatcgat gcctgccctg ctttcgcgag catcatcggg
     6721 atgcactcgt cgccgagcac tgcaccgacg aggcgaatcg cgcgcacgcg gtgctgcggc
     6781 gggatgtcct cgagacagac gagcaacacg cgcgcaaacg cgggcgatgc gaagctctcg
     6841 atcgcttctc cccacagccc aggcgcgcgc gcgaagatcg ccgacgcacc ttcccattgg
     6901 atgtggcgca tcgcgagagg cagctgctcg ctcgcgacct tgccggcgct cgtcgctgca
     6961 tcgccgagct tgatcagcgt ctcgtcggcg agctgcacgg cctcgggcga ccaccgcgtc
     7021 gacggatgca tgcacctcac ctgtcgatag ccgagcgtcc agatcgcctg ctcgcgcacg
     7081 accaccggcg tcgccgaatc gagagccacc gcagcgacgc gcgtcatcgt ctcgaccgtc
     7141 ggaaacatcg tcagccagac gcagccctgg cggcgcatct cgggcgtgtg cttggcctgc
     7201 agcatccagt agccgacctc ggcaagcgac ggatcagcgc cgatccacgg ctcaccctcg
     7261 tcgagcaacc gattcaatcg ctccgatgcg acgccttcga gcggtccacc ttggagcaga
     7321 ccgatgacgt cgcgaatgcg atagcgcacc gccggtggta ctgcgtccat agccccgagc
     7381 tgcacgggca aatccaccac gacgcgagca taaggcggcg tgtcatagtc aggccatcct
     7441 ccatgagccg attcgcgcta gccctgcttc tggtctcctc caccgcgacc gccggctcca
     7501 tcaagggcac ggtgctgttc gagggtgagc ctccggagca gcagacgctc cagcgcgatt
     7561 cagatccgaa atgttcgaag acgaaagcgg acgaagccgt cgtcgtcacg aagggcaagc
     7621 tgcgcgacgt cctggtccgc atcaagaacg gcagcggtgg caagcatgac gctcccgcag
     7681 cgacggtgct gatcgatcag aaagactgca tgtactcgcc gcgcgtcgtc ggaatcgtcg
     7741 ccgggcagaa gctgcaggtg cgcaacagcg acaacacgtt ccacaacgtg tggggccagg
     7801 tctcgaacaa ggatgtcttc aacaagccgc acgccgcgaa ggccgcggac ctgacgctcg
     7861 acccgtcagc cgcgaaggcc gacgacattg tcgagctcaa gtgcggcgtg cacggttgga
     7921 tgcacggcta catcgcggtg caggatcatc cgtatttcgc ggtgaccgcg agcgacggca
     7981 gcttcgtgat caaggatctc ccgcagggca gctacacgct cgaggcgtgg catcccgagc
     8041 tcgggactaa gacgatgaaa gtcgtcatcg gcaagggcaa gcgcgccgac gtcaccgcgc
     8101 ggttctcgta caagcagagc gaacgctaag ctggctactc gaattgccgg ctaatcacgc
     8161 gtgtactgca acgtaccgtt tgcgctgagc acgcacgcgg caccacgctt gctttgacgt
     8221 gcgcgaatgc actcgtctcg tcatctcgta gcagctgctc tggtttcggc cgcaggctgc
     8281 gcaatggagc ccacagaaga tgcaaccgag ccgcagggtg tcggcctgca aggtgcgctc
     8341 gtcaagatgg agatgtcgtc ggacgttgcc gtcttgctcg atgagattcc ggctggaccg
     8401 atgcgcgagg cagccgttgc gaacgtgatg gcgaagcccg cgtccttctg gaccgagcgc
     8461 gcagctcgcc aagtgcggct cacgtactac cggctcgtgt tccgcggtct ctattactcg
     8521 agtgattggg gcaacgcgtc gaaggtgtat ggcccgctcc cgctgcccga caagagcgtg
     8581 tggaacgtcc aactcactgg cacgccgcat cgcgtgtccg cgatcaatgg tcagaatcac
     8641 gactcgatcg tcgttagcta caagttcgac acgatgatcg tcacggacaa ggactcgccg
     8701 ggaaaggtcg acccttccct cgcgacgctc ggcgggaccg ccgacgaagt attcacgctt
     8761 ccgacggatc cggatctgct gctccagcgt accgggtatg catgcatgga tgaagaggag
     8821 tatccgccgg gcagcgtgtt cgaagagaac acctggtact tctacgacga tacgtgcaaa
     8881 gccggtaaca gctggtgcca catcaccgag caacccggcg agtcatgcat caacgcggtg
     8941 aacaagcgcg tcggcgacgt gaagacgacc atgcatttca cgcgcgttcc gtacgacgcc
     9001 accctcgcga gcaagtaccg cgttggtgcg atcacgaacc gggatggcgc cgacctcgcc
     9061 gtcatgcaga aaggcatgga agaagagaag aagatcgcgt accgcttctt cgcacccggc
     9121 tcgtgcgagc tcgaggaagg cgtgatcgcg aagctcgggt ggcggcgcct gctgatgttc
     9181 tcggcgatcg tgcagaacaa cggcacgcag cccgtccaca tcggcgacgt caccgattca
     9241 aacaacccgt gggtccgcag cggcgcattc gagttcagcg cgtgtcacgg ccactaccac
     9301 ttctcgcact acggcacgtt caactacaac ggcgccccgg gctcgaagcg tgcgttctgt
     9361 ctcgaggaca cgaaccgctt ccacaacgac gagactacgc cgctcaccgc cgaccaccag
     9421 agctgccact accaaggcat cggtgccggc tggggcgacg agtaccagtt cggtcttccc
     9481 ggtcagtggg tggacatcac cgacgtcgac acgcgcaaag cccatccgct cacgttcgac
     9541 agcaacccgg acatgttcct ctgcgagggc aagcctgcgc tcgactcgaa cggccagctg
     9601 atcctcgacc cgtcgccgtt caaagatgcg cagggcaaca cggtgtcgcg cgtacgttgc
     9661 aacatgccga cgacgtggca cgacaacaac gtcggcacgg tcagcgtgag ctcgcccggt
     9721 ggcagcttcg tcacggagag ctgcaagctc ggcgagatcg ggcacgaccg cgattgcggc
     9781 tatgccgcgc agacgcagct tcgctcgtgc caagccggct cgacggtcac gctgacgtgc
     9841 aagaacacgg gcgcgccgca agcactgcgc atctgcgaga agagcggcgc cctcggcgtc
     9901 ggagtggcgt gcacgttccg cgactccgtc gcgaatccga ccatcaacgg ctcgacgacg
     9961 ttctcgttca cgtgccccgc cgttcgtgac tatccgggtg cgggcggtta ctcgctctac
    10021 aacgcatcgg tcgtgccgtc gatgggcacg ggttcggtca cctgcaacta gctaccagct
    10081 ctgctggcgg acgtcgcgca gccattcgtc gacggccgcg atggcctcgg gcggtggttc
    10141 cgcagggagc acggagcgcg gccatccggc atcgatggca tcgatcgccg cggcgagccg
    10201 gctccgccat gcacgggcat ggtcggcctc cagcggctgc ttctcgccgc gttgcttgat
    10261 ggccattagc tcgctgatct ccgccggcag atgctcggcg agcttcgcga catcggtcac
    10321 gacgttgccg gtcagcaaga gatgcagccc ggtcgcagcg gtgcgcagga cgtagagcgc
    10381 acgctttgcc gttggcttct cgtcgaacgc gcgcagctgg ctcatcgcaa acccgccgta
    10441 gtgcttgccg acgcggcgcg agatgaccgg agccagcacc gcgcgagcag actcgacgcg
    10501 gacgagatcg ccgccgagca cgagctcacc gaagatgcgc tcgatgtagt tgccgttgcc
    10561 tttcagaatg ccgcgtagca cgccgccgag ctcgttgctc ccatagtcga gctcgacgcc
    10621 gtcgacgata tcgatgcggc ccggcggatc atccggcgga ttgaggccga cgagcgcgcg
    10681 cgtcgacgcg atgtgcacgc acttgaggtc gaggtcgctg tcgggcgacg gaaagccgta
    10741 cgcatgcgcc ccggacaggt acacgacgag gtgcttccgc tcgcgttcgc ggtcggcgag
    10801 aaacgcgcgt gcgcgcggcg cctggtgctc tgtcagaaca gaccacgggt ccatgccaga
    10861 cacggtatca tcagcacgat gcgggtcctc gcactgctcg cgctcgctgc atgcagcaac
    10921 gtcgcggtgc gcgtgcgacc cgccgacctc tcggtccacg cgcaggagat cgtgaacacg
    10981 ggtgaggccg acgttccgtt cgagggcacg acggttcacg taaaagcgag cgagcgtgtc
    11041 gaagtccgac tcgccgatgg cgatacgcag cgcctcgcga ccctgacgat cggtgagctc
    11101 gtgcagggat gtgtgcccga ccccgattcg aagcagtgcc tcgcgaatca agcggtgcgc
    11161 gatccgttcg tcgttcgccg cgagcatcac gtccgctcgg gctcgctcga gactggcgtg
    11221 gcattcggcc tcatgggagg tgccgtcggg acttgcctcg ccgtatgcca ggatcgggca
    11281 gagctcggcg aaggcttggc gtacgtcggc ctcggcgtcg ccgcgttcat gggtctcttc
    11341 ctgctcgtcg tgatggcggg cggccgcgac taacgcggag acgcgggccg gcacgtccat
    11401 gtcagctcgc cgcccatgat cttgtgggct cgacgcacga acgccgtgac gcgcgccatg
    11461 ccgccgaacg cacgaacctg cgagccgtac tcgctaatcg ccgcaagctt ccgctcctcg
    11521 tcgagcttcg caacttcctg cggtgcgcac gtccactcca gccgcgcggc ctccggcaaa
    11581 tactcctcga gccccggccc cttcctcgcc atcgcgacga gccgcagcag cgctcccacg
    11641 cgcgggctcg cccacgcggg cgcgccaaac cagcgccaca tccgccgctt cgcgacgaag
    11701 tgctgcttgc gacacgcgcg cccgagccca tagacatcct cgtagaagcg aatgcgctcg
    11761 aaggcgccgc tgccgagcac ctcgcggagc gcagccagcg ctacctccgc atgatcgacg
    11821 tgatgcccga tcgcgagcgg cgcataaatc gtccgagtgc cgcgctccag gatgtccgtg
    11881 atcaccgcgc ggatcgcctt gagctcgcgg aagtcgtcca tgcgctcggg cgtgcgaaac
    11941 acgtacgtcg gaaagacgcc gcgcgtccgc ggcagtggcg ggtcgcgcca gatgcgctcg
    12001 tgcaaatcga gccagcggtg cgtcgcgccg agtgtcgcca gcgcgcgctc gtcctccgct
    12061 cggcgcatcg agtagtcgcc gaacactcgc cgctccggcg caattgtctc gagtggaggc
    12121 cccgccgtga agcacgagac gatctcgacg cgcttgcccg cggcgacctc gcgcgaaatc
    12181 agcccgccga cggacaacac cgcatcgtcg agatgaggcg acaaaatgac gatgtccggc
    12241 gcggttcgtg cctcactcat cgaagcgcgc ctccggcgga gcagcggagt gagcgcccca
    12301 tgcatcgtcg gtgcactgca cgcagcgccg cgcggcttca gcgcgggcag cgcgcagcac
    12361 tctatcggcg cgcgcgacgt cggggtagcg cggcagcggg ctggtttggc gcgcagcctc
    12421 gagcttcgga gtcagctcgc gcgcgagcgc catgacatcg ggcatcggga cttcgccctt
    12481 ctcgatcgcg atgagcgtgg ggcgcaggct gtccgaaacg cggacggtcg gtggttcggc
    12541 cgagagccag cggattgcga ggtcgaggag gcgaatcagg ttgtaggcgt tcttggggcg
    12601 aaggtcgcgc ggcagggcga tcccgctcgg cggcgcaggt ccgcctcgct ccaactcggg
    12661 ctccgtcttt gcggcggcgc gcagcgagag ccagtcgttt gccgcgatga ggccctggtc
    12721 gtacatcgag cggtagagct gcttgatgta atcgcgagcg cggttgatcg cgtcgtggcg
    12781 cgttggcgca tggacgcgcg cggtgtcggc gaggcgttcg gccgccgcgt cgagctcgag
    12841 ggacgggtca tcgcgcagcc aaccgatgac ggtcgtgcgg tgctcggcga ggc
//
LOCUS       DATLBZ010000506        31129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4360258, whole genome shotgun sequence.
ACCESSION   DATLBZ010000506 DATLBZ010000000
VERSION     DATLBZ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 31129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31129
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4360258"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..608)
                     /locus_tag="VMZ53_25520"
     CDS             complement(9..608)
                     /locus_tag="VMZ53_25520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31896.1"
                     /translation="MPSFLHDVEIDWGWPKLSDEERVLARKHEALLQSDRKSHTVLTA
                     ERALEAAARDVPGRLLRMRAIDDHHTYYWQVVEGEVYPGHEFDDAGMFPGELTAEDFH
                     AAGVTIVMTPIAMPDAMLAEVATRATTLSEVVQDAWREAGDEVPAGFRAPSGPRHMHS
                     IYLPVDVWAELQDRAKTEERSVSYLVQRAVTAAYELPVE"
     gene            complement(625..2175)
                     /locus_tag="VMZ53_25525"
     CDS             complement(625..2175)
                     /locus_tag="VMZ53_25525"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS31897.1"
                     /translation="MSAEDDLKKRAHEAVELLEQLSTELPKLAELDEDLRIRLVTAAG
                     QLSRPDRYSRKALGKALARARVRARREADRAILDQTGIRALRREPVFRTPLPKPTWTA
                     LDAPLRDDEGEADEWAEAEAKRAEGARVEHPRQCYVCKTEFHELHFFYDQMCPPCAEL
                     NYAKREQTADMRGRVALVTGARVKIGYQAAILLLRAGAEVIVATRFPRDAARRFAREV
                     DSADWMQRLHIYGIDLRHTPSVELLAQHLVQTQSRLDAIINNACQTVRRPAGWYDHVV
                     KDEGEAPSTAFERAVLDRNRALLGGDVRMLPAGASQEAGLWKSAVLTQVPLLAEDLER
                     GTHLYPQGRLDQDLQQVDMRDFNSWRMTLADVPTAELLEVHLVNAVAPFVLNARLRPL
                     LAAVPTRDSHVVNVSAMEGQFYRRWKTDKHPHTNMAKAALNMMTRTSAADYVRDGIHM
                     NSVDTGWVTDEDPKAHADRKQQMHGFHPPLDIVDGAARIVDPIFAGLNSGSHLWGLFL
                     KDYKPTPW"
     gene            2219..3526
                     /locus_tag="VMZ53_25530"
     CDS             2219..3526
                     /locus_tag="VMZ53_25530"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HUS31898.1"
                     /translation="MIIAKRLKGKAIAAWLGPTGDAARRAIADVRHKLQKEPSKLELY
                     FDISDPWSYVAAQVASRLIDAYPVDFAFYPVTPPASDVNAHPQLRLKHAVRDAQQLAD
                     YWDIHFPGKKEADPGAVRDVACALIKERPAKEQLRAAIELSHAMWGNDRKTLTSLLGT
                     WGTESTGSVAPILNSNYGQLRNAGHYQGAMLHYGGEWYWSIDRLAHLEAALAKDFNCD
                     VAHVVTAKPESARGPVALSDKPLTLEMWFSFRSPYSYIALEQIESIIAPYDVTLQLRP
                     IMPMVTRGVPLPNVKRMYIVRDAKREADRSGIPFGELCDPLGAGVENCVAIAYWAEKR
                     SQADLLAFSKSAMRGIWSEARDVAEYVDLRHIVERAGLPWAEAKDSIGNPEAAKWATA
                     AVNDLQVIALWGVPSFRIGDFVAWGQDRLPLLADRLRRHKAAM"
     gene            3711..5354
                     /locus_tag="VMZ53_25535"
     CDS             3711..5354
                     /locus_tag="VMZ53_25535"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS31899.1"
                     /translation="MTTKLLPVTGEVTIGRSSTCDLMISHISVSRHHATLRVSPLQIT
                     DHGSRNGTRIRGRALPSGTATAISAGDAIQIGDAAILLQPVMMSFDGKPLVEPVLRTE
                     GTVQALDTECARSARTGSPFALIQIATEQGKPKDIVALLRGFLRTTDIVRGDSTGGFS
                     VLLPDTADVTIAVARLRDLLRQHGIAARFGAARYPDDGVSPEQLLAYAFEELAREPDA
                     DPTPMDTVRALVGSVAGGELSVLINGETGVGKELCAEMIHRLSPRAKKPFVKLNCSAL
                     VESLIESELFGHERGAFTGATSAHPGLLDTGNGGTVFLDEIGELPVGVQAKLLRVLEE
                     RVVRPVGATTGKKVDVRFIFATNRDLVEEVNAGRFRRDLYYRMNGVTILIPPLRERRG
                     EIAALARAFADRARPGVPLMLGKEVVAALEHHSWPGNIRELRNTIERAVLLSSGGAIR
                     PAHLVLERDSPPAVRDSQPTMPVERLSSDSLTLERPAIRQSSQSLASEVADLERQRIL
                     QALEECGGNQSRAAKLLGMSRNTLLSRLDEYGLPRPRKA"
     gene            5471..6340
                     /locus_tag="VMZ53_25540"
     CDS             5471..6340
                     /locus_tag="VMZ53_25540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31900.1"
                     /translation="MRITPAAAIVLLAACADSAPEAQKDVPGTVNVDEDTESRIAGSY
                     VAGDDTLLFDASEIDTNLISVTVKLHGLTIDATLDTRDGNRMWSQDAFATDTGADSVI
                     TEDDQEMMARFVKALEAENIGISEGNGLAFHFGTVINLWAEWTPAMDPVRIKFEDRDR
                     AVDMCWFASAPDRYHDYDGHDCSSCSGDPGQGQGRTNCSSYVAYGPYYAGTTYYYKGG
                     TWVSGDDMDGHGNYDYQTGNCYGRYGGGCGSGSAYFRENASHDHCVRNGHVIGSSWCS
                     DELWNTTQPYNCF"
     gene            6340..6690
                     /locus_tag="VMZ53_25545"
     CDS             6340..6690
                     /locus_tag="VMZ53_25545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31901.1"
                     /translation="MRTALAFVMLVACARSEPSERELLSGAQDATRTFFALGEGGDCK
                     QLAPMLQRPATCDDLVRQFRDTHAHLSQVLGAKIDGRDKHMVLVSVDATAPEHVHHWI
                     VRAKWTSSGWKLAL"
     gene            6687..7208
                     /locus_tag="VMZ53_25550"
     CDS             6687..7208
                     /locus_tag="VMZ53_25550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31902.1"
                     /translation="MRVRTLAPTLLALGALVAIYVFARHGDEPSTPSPSAAQNVHAPA
                     PAAAPPSAPPPAITAAPLPASGDMPVAPPKALTVDEYAGHEPKAKKPKMTLQEELAET
                     TKHISVMEKRATLIDQQVAELEKAGKTKEAAEQRVIAERLRKHVEQLREAVAAGRAPN
                     ETSDSKSGDEHTP"
     gene            7205..8278
                     /locus_tag="VMZ53_25555"
     CDS             7205..8278
                     /locus_tag="VMZ53_25555"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HUS31903.1"
                     /translation="MKRVVACLLLAAGCDVTTGQQTHAIVGGQLDPDDLSIVAVVARR
                     TQCNQPSVGIQCTATVIGEQVALTAAHCVTQDAAGTLEVYIGAHVGDPGGRFLLVDDV
                     MRHPAFDDETHAYDIALLHLAERTIVPPVDLPLARLDDSSVGARTRVVGYGVTQAGVL
                     ADGQRRETTMLVSAVDAHTFTATPDPGNSCGGDSGGPVFVATPTGEQLLGVTSAGDPL
                     CATRAVATRVDVALDFIRTYVLSPPLIAPATIDPAETCTAMCSTSAECPAAMVCDLGR
                     CHLAGALPASYHDTCTQDADCDGTCAQLSPNECRCAIACSMVGGPGSPGDDDTCNASG
                     RSSWLVLLALAFMVRRSRRRTAV"
     gene            complement(8241..9800)
                     /locus_tag="VMZ53_25560"
     CDS             complement(8241..9800)
                     /locus_tag="VMZ53_25560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31904.1"
                     /translation="MSRRALLWTIAGVAVAVVCVLAAVTAWTCDDALISFRYVRHWVG
                     GDGLVFNIGERIEAFSNFLFVMLLAPFAAAHADLFTVANVLGIVATAVQVALLVWLLG
                     RRGLPLVAVAFSAALFATDRIAVVWATGGLETATHGALVLAALALQLERPTAIRMTAV
                     VHVALAASRPEGVMFAAIWFAHLLATRQTAELRRALNWFVPLVVALVAARLLYYGELV
                     ANPYRAKVSGVATLDFGPGYVSMFLRRMGLWGLAALATLPLLVSGLRTRPDRALLLGA
                     AFVIAQLAMAAWTGGDYMTDFRILAPVVGVFYVVVGLLAARAWSSKTLAIVAMVAFAG
                     GHAYRQLVPVPVFSTAPAPAAHKRILTVTREQPSAFARALSVFAEPGDSVLADWGGWM
                     SYGHDLRTIDATGLVSKRITRDFYLRSKDEQLPGHARWPTIELMQREHLTFVLPKVNE
                     RPPEDAEINEQTPVRRQEYPFLHVTIPLEHGAYLRFFTTLSADELIARGAAKHVRVCF
                     RPPFGALTCAP"
     gene            9925..11994
                     /locus_tag="VMZ53_25565"
     CDS             9925..11994
                     /locus_tag="VMZ53_25565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31905.1"
                     /translation="MGSAEEFGPYVVHEQIGLGGMATVHRAETQGIAGFSKQVALKRM
                     LPNVAADANLVKSFIREARLASHLRHANVAQTYDLGKVGETYFIAMELVPGWNLREIL
                     KHSAQTKRNMPLPIALNIVNQICDALDYAHNLCDETGQPLGIVHRDVSPSNIILGEGG
                     VAKLIDFGIAKASTSGMQTMSGTIKGKFSYMAPEYLMGYLDWRGDLFAVGVIAHELLS
                     NKPLFQGKDDMDTLYRVKDMPILPPSRFNPLVPPEIDNIVMTALERDPNRRWQQASAM
                     RSALTTETARLSLVAQNQEIDVWLDQTFAGGADDLEAPEISLGGSTMELSKGSVPDDF
                     LNAAADSVSTLVRPVGSQPIQAQGTPVARDSSPTLGDRPSRQMNAQKSRAESPRPPSR
                     PPSRPSQNVVPRGEFGDTAQNRDWGDDPPTRADASAVDQIAQSSLARRDDQRTMKGAG
                     IDGLSQRTLVSAPPAELLAQQSFTEETVANAAAMRSLADSPLARDESLEAPTLTNTKR
                     ATTDVTDADGVAAESRRDEFAQHVDTTPRRPTPRPQPAASPLTNVPKIRDEATTTQDA
                     PPRRDSGLRPSSPSAPPAPGPLRTTLGVSAPPPIVHSGARTRPHIPKASAEIEVGSSA
                     IVAEVSVSDVLADLGHRPTAHTPAKGAAMPMAPKSGGSSFLLMLLVLIVAGGAAAVVY
                     FALPYFT"
     gene            12010..12522
                     /locus_tag="VMZ53_25570"
     CDS             12010..12522
                     /locus_tag="VMZ53_25570"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HUS31906.1"
                     /translation="MNKRRIALISTGGTIEKTYDELSGVLANQVSVLDVMLASLELRG
                     VEVQRVALMNKDSLEMTPDDHALIATTAADMAKKHAGVVIVHGTDRLAVSGDRICELG
                     TPASPIVLTGAMRPYELRSTDALQNLTEALLAVQILQPGVYVAMHNKVLQFPGVTKDR
                     NAGTFVKEGA"
     gene            12519..13619
                     /locus_tag="VMZ53_25575"
     CDS             12519..13619
                     /locus_tag="VMZ53_25575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31907.1"
                     /translation="MSELPIALLPALLGALDRAGVGVVAMQYSGDATSKLYANESLAT
                     SLGYTPQEWRATPLWHVISPRHRPAINALFEKLARTPDEPMPTAIEVMLVHRDGHEIA
                     AEVAAGRVEVAGGYAFVFIVRDMSLHRQTQLSLLEADRLALVGALASGFAHETNNPLT
                     SVLLNLRSLRKQVHAHMPEQSQTTALRCLDDITTGSERIASNVRALQTLATRSTTATI
                     DLAMVVSSALRLAAPTLEPRAHVIRQIFPVAPVLGEESRIGQAVLAMLLFASSGFDTD
                     VAVTSNRIVVAVEDRDGKVCVEVTDNGRALTDVDAEHAFDPFFRTPVRGAGVGVGLGV
                     ARSVAVTLGGEVALTRRDGGGAVVTMRLPPAP"
     gene            complement(13909..14352)
                     /locus_tag="VMZ53_25580"
     CDS             complement(13909..14352)
                     /locus_tag="VMZ53_25580"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HUS31908.1"
                     /translation="MSLVERLEEAKRSGDCQALFDIVPYSRFLGLSARLDGEELITTM
                     KFSPELIGNPALPALHGGTLGALLESSAIFELLWRAETIVLPKTITITVDYLRSGAPV
                     DTHARSIVTRHGRRVANVRVEAWQTDRKTPVATAHAIFLVMRDPP"
     gene            complement(14349..14852)
                     /locus_tag="VMZ53_25585"
     CDS             complement(14349..14852)
                     /locus_tag="VMZ53_25585"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HUS31909.1"
                     /translation="MASDDKPLRVSRGFVDGIPHNKALGIKVVEFRRAEAIFELPYDT
                     KLVGNPDTGVLHGGAITALLDACSGAAVFAALTEWQPIATLDLRIDYLRTGDVDRAIR
                     AKATCYRITRNVAFTRAIAYHDDDEADPIASSVGTFMLGTKPAGGVGGTGLGTTGPAS
                     GSKKDAP"
     gene            14875..15333
                     /locus_tag="VMZ53_25590"
     CDS             14875..15333
                     /locus_tag="VMZ53_25590"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUS31910.1"
                     /translation="MAGRAPIPTWYFALVVVRRGQRFLLTQERKYGSSWSIPGGRVEP
                     GESLVRAAIREVYEETGVPVRVDGILRVEHSAAESTVRMRVLFTGTPLDDTPPKTTAD
                     DESLGAAWLTLDEIRKLPLRGAELRALLESVESGRQVFPLDLLGDSEMSI"
     gene            15346..16392
                     /gene="rsgA"
                     /locus_tag="VMZ53_25595"
     CDS             15346..16392
                     /gene="rsgA"
                     /locus_tag="VMZ53_25595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HUS31911.1"
                     /translation="MGWDTDWDDARRAVDPDCVLHPVRVVAEHRGAYHATNGDGVGWI
                     ELPGRTYHRAHDKRELPTVGDWVMVERWPEALASNGAAVIRHLLPRRGLLVRKAAGEA
                     TVPQPVAANVDIGIVMTSANSDVSPARIERYLSLLSDGGITPALMLSKIDLVADAGPL
                     LDELRAIAPGHTVLAVSKVTGAGIDEARALVGPGRTAVLLGSSGVGKSSLLNALLGEG
                     TQLTQPIRDDDRGRHTTTRRELFITEYGLWIDTPGMRELAQFIDEDDPELVFDDIDAL
                     AEQCKFRDCQHRAEPGCAVRDHIDPRRLASFHKLLEERATGAEKQNVARRLAETRRAK
                     ANKYAPRPGKKDQD"
     gene            16432..18042
                     /locus_tag="VMZ53_25600"
     CDS             16432..18042
                     /locus_tag="VMZ53_25600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HUS31912.1"
                     /translation="MIAFSGVTKQYGGQILFVDASFQINPGEKVGLVGPNGAGKSTVF
                     RLIVGEETPDDGNVEKPRKLTVGYFRQDVGELRGRTILAETCAGAGEVAELADELARL
                     TTALETDPTDEVVTRYGEVQSRYQDLGGYELEARAQEILNGLGFHQEQMSADVGTLSG
                     GWKMRVALAQILLAKPELLLLDEPTNYLDIESILWLETFLRDYAGTVVMTCHDKEIMN
                     RVVKKIVEIDGGALRAYGGNYDFYEGAHKIEAERREAEYARQQAMLAKESRFIERFKT
                     HVAKAAQVQSRLKKLDKIEKIAEPKRFIEKSFDFKTPSRSGDDVVRVEALRKAYGTRV
                     VHDNLTLTVRRRERWAVMGENGAGKSTLLKMIAGALPPDAGVAAIGASVQMGYYAQHV
                     MDLLTSDRTVLEELQEHAPSANQGTLRSLLGAFGFHDDDVFKPISVLSGGEKARVALS
                     KLLYDAPNLLVLDEPTNHLDIATKRSLVKALADYEGTLIFVSHDRQFLSALATRVLEL
                     SPQGARVYGGSYDEYVASTGREAPGMRA"
     gene            18117..21248
                     /locus_tag="VMZ53_25605"
     CDS             18117..21248
                     /locus_tag="VMZ53_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS31913.1"
                     /translation="MAADDDDPRRQGGAPPPQKKTQFGMVIPTPSGAPQAPQAPRVPA
                     ATPQPPATNKTTFGVPSPMARPPAQRVTPAPMPVQRVTPATTPAQPQPQPPPSNATTG
                     TAHGQATDQDLPHDVIASGMGLEPGGRVHQYELIRELGRGGMGMVWAARDTKLGRRVA
                     IKFLLDASRSVADRFLTEARATAQCNHENIVIIHEVDEIESMPYMVLEFLEGQPLRGV
                     MGTYGSGMRLAPTRVVELMLPVARALARAHELGIVHRDLKPENVFVTNAGQVKVLDFG
                     IAKAVARRDPQAQQRSVYGDLHAQGANLNMTREGALIGTLPYMSPEQMGIGEVDHRSD
                     IWAFGIMLYEMLAGRHPVYPLTSEALITNAVSEDAMPSVRSDVPDVPDALAWIVDSCL
                     RKRVVERMPRATDLVKKLEELMPGRAGRQLAEGESPYPGLTAFQEADADRFFGRTREV
                     ARMTARIREFPITGIVGPSGAGKSSFIRAGVGPALKASGEHWEVVTLRPGRQPLAALA
                     SIVQRLTTRSGVDVQTQVADHHTLLQRLRGEPGFVGTLLRNRARQTGTQILLFVDQFE
                     ELYTLVPDPNERRAFTAALTGVADDTAAPLRVVVSMRSDFLDRIAEDQRFMEELSRGL
                     VFLQPPDRAGLREALVAPIEMVGHRFESAAMVEDMLDALVNTPGALPLLQFAAAKLWD
                     ARDRQRRLLTVASYNSIGGISGALATHADDIVAQMNDRMQRLTQKIFRRLVTPERTRA
                     IVELADLYQLAPDQAEVSRTIDQLVSARLLMVQTRGDTGSSGGGSVEIVHESLIDRWP
                     TLRRWLDEDQEDAAFLSQLSSAAKQWEAKGRSRGLLWTGDAMEEARRWSAQRPRELPA
                     REQAFLDAVFSHAQRGKRAKRALLIATFALLGIVAAGASVAAVKIRDSEQQAKTALSQ
                     IQQKEAERDRAEAAQKAAETEKARAEEAKQKAELDKQKAELEKHKAEQDKLAAEAMSR
                     EQLQAKNLELQKTLDEVQTAKATAEKAKEIAVKEKQNAERARDEANAAKAKIQAELDR
                     QRQENAKLSHKLK"
     gene            complement(21458..22456)
                     /locus_tag="VMZ53_25610"
     CDS             complement(21458..22456)
                     /locus_tag="VMZ53_25610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS31914.1"
                     /translation="MKLRTLCLVVGLWVATANIAHADQPWAQGVSEAQKAEAQKKLEE
                     GNALFLKQNYPEALKAYQAAVAQWDHPAIRFNIVRCLIQLDQPVPASDNLQLALKYGA
                     APLEEAVYTEALAYQKLLANQIAEVEISCKQDGAKVTLDGETLIAKCPGSEKRRVKPG
                     QHGVVTTKEGFLPQNIEVVVVGGNTETVETNLVPLAKAAKIVHRWPTWIPWVVFGGGL
                     VVAGFGGVIEATAFSNMSTFDKQVSSNCQTTGCDLNTNQSLRDQRDDAKTLGNIGIAI
                     ISVGAAGAVAGGVLLVMNRGQTVYEQPEKQQGPLGTARLDYVPHDGGGVLTLSGRF"
     gene            complement(22502..23368)
                     /locus_tag="VMZ53_25615"
     CDS             complement(22502..23368)
                     /locus_tag="VMZ53_25615"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS31915.1"
                     /translation="MALVADPVYVLGPRKKDALATRADALVTLREILDAEKDKKFPVK
                     SGNLSVVASQGGLSAWAVDTIDVQGETMAVTVVLSNADDFWVVAAASVAVTPSMKSVR
                     ASLAKDAVVPPAMEAPGSVPDAAEAAADRFKRGFGDPTVWGDDLSKRGDAVVIGPAAE
                     EITRGKTAIAKLWKKRTKANVRYASAGDTLAGTTPDGQIAWVSAPVVRFEDNEDQPLP
                     LRLFGVYEKAGSDWKLISLQESLAIDEPGQGASFKKIAAPAVKQDEPPPKPKTEDKPK
                     KKTKKKKKSSDD"
     gene            complement(23533..24483)
                     /locus_tag="VMZ53_25620"
     CDS             complement(23533..24483)
                     /locus_tag="VMZ53_25620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31916.1"
                     /translation="MFALAHWLHTEARMGALDNFLRRFGYAKLDRYGLILTGDDRVLS
                     TRPAVLDDGLGGKIVGWTDDDLAAMELEHIGVPKKKKPAKLVAAPASLHKLPVAPPPV
                     PLAAKIVAPAAKLPGLPPTGPIVIAPVVAPVAPPVAVLPPKVAEEPPVEEDEWEWDIM
                     MAKARAAAEEVEQAAASIDSALPSGVLRDPAKPGLAIATSVTAPRRKTSPSIPAVRAK
                     TEPPVRTMPKPVAPIAERKTVIPVPTLQATAKPSELQPYTPYSPPRRSPRGTGSVGED
                     TVRTEAVPANDDHTSPYVTMPAEVKPVGYAHTKRVAAKER"
     gene            complement(24615..25394)
                     /locus_tag="VMZ53_25625"
     CDS             complement(24615..25394)
                     /locus_tag="VMZ53_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HUS31917.1"
                     /translation="MSDILVEEAQGVLWVTLNRPEVRNALSRDVNVRLQEIASDLEQR
                     HDEIRIVVLTGAGDKAFCAGADLKERKGIPAAEAGPYINAIANAIEGWGAIKQPTVCL
                     MNGSAYGGGLEIAMACDFRIIVENAEVGLTEVKLGIMPGAGGTQRLPRLIGEARAKEL
                     IMLGRRIHAGRALEIGLVHQVVPRDRLRAAGDALLAELATCAPISVMSAKSAIELGFG
                     KPMEEGLTIERECYERTLYSEDRDEGLAAFAEGRPPKYQGR"
     gene            complement(25407..26345)
                     /locus_tag="VMZ53_25630"
     CDS             complement(25407..26345)
                     /locus_tag="VMZ53_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HUS31918.1"
                     /translation="MKLPAQVTIYEVGPRDGLQNEARNVPTADKIRFIDALVASGIKS
                     IEITSFVSPKWIPQLADAAEVARGVQRPPGVLMSALVPNRRGLDTALASGMKVVAVFL
                     SASETHNKKNVNKTIADTLVAFDEVIPAARAANVPVRAYVSTVFGCPYEGDVDPEKTV
                     ELVGKLRDMGVYQVSLGDTIGVANPQQTEDVIGRVLAKHPADSLAVHFHDTQGTALAN
                     CLVSMQMGITTIDASAGGLGGCPYAPGASGNLATEDVVAMLHSMGVKTGIDLDKLTEA
                     SRTASTFVGHELPSKYLKAHLGKQARARRQAEKLGS"
     gene            complement(26342..27031)
                     /locus_tag="VMZ53_25635"
     CDS             complement(26342..27031)
                     /locus_tag="VMZ53_25635"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HUS31919.1"
                     /translation="MIRGFLFDLDGTLVDSERESGEAMARALSRGQGVEIEQSDRDYV
                     IGRSWVAIYDRLKERYPQIEWNRNELIARTAMLREEVFAETGVTIMPHARETLTWTRT
                     YPRALVTGSSRVEVTQVLPLIGEMAAFEVIVAAEDVGRSKPHPDGYQKALDALGLDAK
                     HCVVVEDSTAGIEAGRSAGCLVIAVRAGNFGGWDQSGAHHLIDTLADLTPPLVDKLAR
                     DYGFGMYGGAL"
     gene            complement(27067..28002)
                     /locus_tag="VMZ53_25640"
     CDS             complement(27067..28002)
                     /locus_tag="VMZ53_25640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31920.1"
                     /translation="MKKLIVVLGVSLLGCGADNSGPEMLPELAAELEAPPANGIQVVS
                     PIFEDVEPGTDYEICTWTDAVFDKQTDVRWTQGFQSEPPGHHVILFYTLDKQPPGTQR
                     ICTDNDMATFRFLAGNGGNKEKNMAPGNLVYRIPPGAQIVMNHHYLNASDEKLKGQSA
                     VNIAFADAGDWIPSGNTAIVNTGLEVQPGMTTQEMHCTIDRQMKLWYFAPHMHRWGKE
                     ISIDVTKSGEKTRMFDIHDWDPSWTFHPPENRRDPADPLILNPGDKVDVSCTWNNTEG
                     RVLPFGFEMCVAFGQFVDDAHMGNWACDHGNWVDF"
     gene            complement(28015..28356)
                     /locus_tag="VMZ53_25645"
     CDS             complement(28015..28356)
                     /locus_tag="VMZ53_25645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31921.1"
                     /translation="MRAAVLIIMLTVAACGGDDSSSTPMDAAVDAYYSLCGHPGDPGN
                     ELGIGKFCATLSDCNGTMSAPLCSSLGDKDTHFCTKTCSMGSTNACGTGAECTCNGNN
                     QCGCTPSVCLN"
     gene            28457..29872
                     /locus_tag="VMZ53_25650"
     CDS             28457..29872
                     /locus_tag="VMZ53_25650"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS31922.1"
                     /translation="MHRPCVMLAKMLRVRVVLALALLTPAACANRAKQSVSLYESGDY
                     AGAARAADEGLAQHPDDDGLWAMKVRAALALGDADAMAKAYAGYMAHRGEDDKELLRD
                     LSQATIAQALASPSVKLKIEAIEAVAALELHDLADAVAERMDDQDDRVAAAAAVAVLK
                     GFPEAPRIADEMLHSDVSEARRIVVDGIGKKVGKLALSDLEKAAADRDHNVRRAAIYW
                     LGALKDKDAVEVLTKRMKDPDEGVRAAAAQALAKIGIGHLDAYAKQALADRALSVRMA
                     GVDLLAAAKRDADLIALTDDKDPMVALSAAIAVKSSRPELAAKAIDRAITAEEWTVRA
                     GAANMLGMAVDKATAASYAQKLVNDTAVGVRLAAARVLLHQGDRTTATPIFSAALGDA
                     DFGLQAATDLASINDKAGLDALAAATRDTTKTPEQRAAAASAHRSAHRVTPGLVAALA
                     DPNGLVRIEAASTLGALAKSK"
     gene            29942..30436
                     /locus_tag="VMZ53_25655"
     CDS             29942..30436
                     /locus_tag="VMZ53_25655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31923.1"
                     /translation="MRRWVPSRCFLAFAFAFAYALALAACHSKPATPTTTANARVTLA
                     VLPAESDAFPKAARAVTESLERASVSGIDDKNISKVSLEVVQLSIECVEPTPACYKAV
                     GRSLSANRLLFAQISAEKKKAVKVSVTLFDVESQSPRTAEKVFPNEKAATDGIQDLVA
                     EATR"
     gene            30433..>31129
                     /locus_tag="VMZ53_25660"
     CDS             30433..>31129
                     /locus_tag="VMZ53_25660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31924.1"
                     /translation="MSTKKRTDETPPSDVVEMDAMDEAEWEKDQHTPMVQDSELQKLV
                     KESADTPAKGVATKPSGSGKPATGTAKTVKRAPTSAGTASSTTTAAAPTTPAPTTTAP
                     ASAPTKSDAAPSGRAKRPTKSDAKSKPASSAPPASPASPASPASPAPPASPAAAAPAP
                     AAPAAPADDDAWFELTDGDDAPSPPKVETGPVGAKPGSRTITTNTVSRSGATKPDASK
                     SAPASASPTSAKPT"
BASE COUNT         5387 a        10555 c        10145 g         5042 t
ORIGIN      
        1 ggcatgggct actcgacggg cagctcgtac gccgcggtca ccgcgcgctg cacgagataa
       61 gagacgctgc gctcctcggt cttcgcgcga tcctggagct cggcccatac gtcgaccggc
      121 aagtagatcg agtgcatgtg acgcggcccg ctcggcgcgc ggaagccggc gggaacttcg
      181 tcgcccgctt cgcgccacgc gtcctgcacg acctccgaga gtgtcgtcgc gcgcgtcgcc
      241 acttcggcga gcatcgcatc cggcatcgcg atcggcgtca tcacgatcgt gacgcccgcg
      301 gcgtggaagt cttcggccgt cagctcgccg gggaacatgc cggcgtcgtc gaactcgtgg
      361 ccggggtaga cctcgccttc gacgacctgc cagtaatacg tgtggtgatc gtcgatcgcg
      421 cgcatgcgca gcaggcgtcc cggcacgtcg cgcgccgccg cttcgagggc acgctccgcg
      481 gtcagcacgg tgtgcgactt gcgatccgac tgcagcagcg cttcgtgctt gcgagcgagc
      541 acgcgctctt catccgagag cttgggccaa ccccaatcga tctccacgtc gtgcaggaag
      601 ctcggcatgg cgcggagcct acatctacca gggggtcggt ttgtaatctt tcaggaacag
      661 accccacagg tgggatcccg aattcagccc ggcaaagatc gggtccacga tgcgcgcagc
      721 accgtcgacg atatcgagcg gcggatggaa gccgtgcatc tgctgcttgc ggtcggcatg
      781 cgccttcgga tcttcatcgg tgacccagcc cgtatcgacg ctgttcatgt ggatgccatc
      841 gcgcacgtaa tcggcggccg atgtccgcgt catcatgttg agcgcggcct tcgccatgtt
      901 ggtgtgcgga tgcttgtcgg tcttccagcg ccggtagaac tggccttcca tcgccgagac
      961 attgacgacg tgtgagtcgc gcgtcggcac ggcggcgagc agcggacgca accgcgcgtt
     1021 gagcacgaac ggagcgaccg cgttgacgag gtgcacttcg agcagctctg cagtcggcac
     1081 gtcggcgagc gtcatgcgcc acgagttgaa gtcacgcatg tcgacttgtt ggagatcctg
     1141 atcgaggcgg ccttgcggat agagatgcgt gccgcgctcg agatcctcgg cgagcaatgg
     1201 cacctgcgtg agcacggcgg acttccagag cccggcttct tgcgatgcgc ctgcgggcag
     1261 catgcgaacg tcgccgccga gcagtgcgcg gttgcgatcg aggaccgctc gctcgaacgc
     1321 cgtcgacggt gcctcgccct cgtctttgac gacgtggtcg taccaacccg caggacggcg
     1381 gaccgtctgg cacgcgttgt tgatgatcgc gtcgaggcgc gactgcgtct gcaccaggtg
     1441 ctgcgcgagc agctccacgc tcggcgtgtg ccgcagatcg atgccgtaga tgtgcaggcg
     1501 ctgcatccag tcggcgctat cgacttcgcg agcgaaccgg cgcgcggcat cgcgtgggaa
     1561 gcgcgtcgcg acgatcactt ccgcgccggc gcgcaacaag aggattgcgg cttggtagcc
     1621 gatcttcacg cgcgcgccgg tgacaagagc gacgcggccg cgcatgtctg cggtctgctc
     1681 gcgcttggcg tagttgagct cggcgcacgg cgggcacatc tgatcgtaga agaagtgcag
     1741 ctcgtgaaac tccgtcttgc agacgtagca ctggcgcgga tgctcgactc ttgcgccttc
     1801 cgcgcgtttt gcttcggcct cggcccactc gtctgcttcg ccttcgtcat cgcgcagcgg
     1861 ggcatcgagc gcggtccacg tgggcttcgg cagcggcgtg cggaacaccg gctcgcgacg
     1921 cagggcgcga attcccgtct gatcgaggat cgcgcgatcg gcttctcgac gggcacggac
     1981 ccgcgcgcgg gcgagcgcct tgccgagcgc cttgcggctg tagcggtccg ggcgcgagag
     2041 ctggcccgcc gcggtgacga gcctgatgcg cagatcctcg tcgagctcgg cgagcttggg
     2101 cagctcggtg ctgagctgtt cgagcagctc gaccgcttcg tgcgcgcgtt tcttcagatc
     2161 gtcttcagca gacatggggg tgtgagcgcg gtggggaaga tgtaatgtgc cccgccgcat
     2221 gatcatcgcc aagcgtctca aggggaaagc catcgccgcc tggctcggac cgaccggtga
     2281 tgccgcccgt cgtgccatcg cagacgtccg ccacaagctc cagaaggagc cgagcaagct
     2341 cgagctctac ttcgacattt ctgatccttg gagctacgtc gctgcgcagg tcgcgagccg
     2401 cctcatcgac gcgtaccccg tggacttcgc gttctatccc gtcacgccgc cggcgtcgga
     2461 cgtcaacgcg catccgcagc tgcggctcaa gcatgccgtc cgggacgcgc agcagctggc
     2521 cgattattgg gacatccact ttcccggtaa gaaggaagct gatcccggcg cggtgagaga
     2581 cgtcgcgtgc gcgctcatca aagagcgtcc tgcaaaggag cagctgcgcg cggcgatcga
     2641 gctctcgcac gcgatgtggg gcaacgatcg caagacgctg acgagcctgc tcggcacgtg
     2701 gggcaccgag tcaaccggct cggtcgcgcc gatcctcaac tcgaactacg gccagctccg
     2761 caatgcgggt cactaccaag gcgcgatgct gcactacggc ggcgagtggt actggagcat
     2821 cgatcgcctc gcgcacctcg aggccgcgct cgcgaaggac ttcaattgcg acgtcgcgca
     2881 tgtcgtcacg gcgaagcccg agagcgcgcg cggtcctgtc gcgctgtccg acaagccact
     2941 cacgctcgag atgtggttct cgttccgctc gccgtattcg tacatcgcgc tcgagcagat
     3001 cgagtcgatc atcgcgccct acgatgtgac gttgcagctg cgtccgatca tgccgatggt
     3061 cacgcgcggc gtcccactgc cgaacgtcaa gcgcatgtac atcgtgcgcg atgcaaagcg
     3121 cgaagcggac cgctcgggca ttccattcgg cgagctctgt gatccactcg gcgctggcgt
     3181 cgagaactgc gtcgcgatcg cctattgggc cgagaagcgc agccaagcgg acctactcgc
     3241 gttctcgaaa tcggcaatgc gcgggatctg gagcgaggct cgcgacgtcg ccgagtacgt
     3301 cgacttgcgg cacatcgtcg agcgcgccgg tctgccgtgg gcagaagcga aggattcgat
     3361 cggcaacccc gaggcggcga agtgggcgac ggcagcggtg aacgatctgc aggtcatcgc
     3421 gctctggggc gtcccgtcat ttcgcattgg tgatttcgtg gcgtggggac aagaccggct
     3481 gccgctgctc gcggaccggt tgcgacgcca caaagcggcg atgtagcggc aagccccaca
     3541 ctgggccggc gtctactacg aagaccgctg attcaatgga attacgcgat cgcttacact
     3601 cgtagcgata cgcagtgtca aacgactttg acgacatcga gaccgatggg tcaaggggag
     3661 ccgatgttgg aggaacacgg cttcacgcgt tgatcagcgt cgatggtgtg gtgacgacca
     3721 agctcttgcc ggtcaccggc gaggtgacga tcggtcggtc gtcgacctgc gatctcatga
     3781 tctcccacat ctcggtctcg cggcatcacg cgacgctgcg ggtgtctccg ctgcagatca
     3841 cggatcacgg aagtcgtaac ggaacacgca ttcgcggacg cgcattgccc tcgggcacag
     3901 cgaccgcgat ctcggcgggg gatgcgattc aaatcggcga cgcggcgatc ctgttgcagc
     3961 ccgtcatgat gtcgttcgac ggcaaaccac tcgtcgagcc agtactccgc accgaaggaa
     4021 ccgtgcaggc gctcgatacc gagtgtgcac gttcggcacg aaccggatcg ccgttcgccc
     4081 tgatccagat cgcgaccgag caggggaagc cgaaggacat cgtcgcgttg ctgcgcgggt
     4141 tcttgcgcac gaccgacatc gtcaggggcg actccaccgg tggattctcg gtgctccttc
     4201 ccgataccgc agacgtcacg atcgccgttg cgcgcctgag agacctactg cgccagcacg
     4261 ggatcgccgc gcgcttcggc gccgctcgct atcccgacga cggcgtctcg cccgagcagc
     4321 tgctcgcgta tgcattcgaa gagctcgcgc gagagcccga cgcggacccg acgcccatgg
     4381 atacggtgcg cgcgctcgtc ggtagcgtcg ccggcggcga gctgtcggtc ttgatcaacg
     4441 gcgagaccgg cgtcggcaaa gagctgtgcg cggagatgat ccaccgcctc tcccctcgcg
     4501 cgaaaaagcc gttcgtgaag ctgaactgct ccgcgcttgt cgagtcgttg atcgagagcg
     4561 agctattcgg ccacgagcgc ggtgcgttca ccggcgcgac gagcgcgcat ccaggtctgc
     4621 tcgacaccgg caatggcggc acggtcttcc tcgacgagat cggagagctt ccggtcggcg
     4681 tgcaggcgaa gctcctgcgc gtcctcgagg aacgcgttgt tcgccccgtt ggcgcgacga
     4741 ccggcaagaa ggtcgacgtg cgcttcatct tcgcgaccaa ccgcgatctc gtcgaggagg
     4801 tcaacgcggg acggtttcgc cgcgacctct actatcgaat gaacggcgtc acgatcctga
     4861 tcccgccgct gcgcgaacgg cgtggtgaga tcgcggcact ggcccgtgcc ttcgcggatc
     4921 gtgcgcggcc cggcgtaccg ctgatgctcg gcaaagaggt ggtcgcggca ctggagcacc
     4981 actcgtggcc cggcaacatt cgcgagctgc gcaacaccat cgagcgcgcg gtgctgctgt
     5041 cgtcgggcgg cgcgattcgg cccgctcatc tcgtgctcga gcgtgatagc cccccggccg
     5101 ttcgcgatag ccagccgacg atgccggtcg aacggctgtc gagcgattcg ctcaccctgg
     5161 agcggcccgc gatccggcag agctcgcaat cactcgcgag cgaggtcgcc gatctagagc
     5221 gtcagcggat cctgcaggcg ctcgaggaat gcggcggcaa ccagtcgcgc gcggcaaagc
     5281 tgctcggaat gtcgcgcaac acgttgctct cgagactcga cgagtacggg ctgccgcgac
     5341 cgcggaaggc ttgagcaacg ggacccttct ttgggcaagt tgcccaaggg ctgagcaatc
     5401 cggaatcctc gcaaggcgtg tctttcccgt gcgttagcgc tcggtacgtt cgttgctcac
     5461 tgcgggcgtc atgcgcatta cgcccgccgc tgcaatcgtt ctccttgccg cgtgtgccga
     5521 ctctgccccc gaggcgcaga aagacgtacc cggcaccgtg aacgtagacg aggacaccga
     5581 gtcgcgaatc gcgggctcgt atgtcgcggg cgatgacacg ctcctgttcg atgcgagcga
     5641 gatcgacacg aacctcatca gcgtgaccgt caagctgcac ggcctgacga tcgacgcgac
     5701 gctcgatacc cgcgatggca accggatgtg gagtcaagac gcgttcgcca ccgataccgg
     5761 cgcggactcc gtgatcaccg aagacgatca agaaatgatg gcgcgattcg tcaaggcgct
     5821 cgaagccgag aacatcggca tctccgaggg caacggcctc gcgttccact tcggcacagt
     5881 catcaacctc tgggccgagt ggacgccggc gatggatccc gttcgcatca agttcgagga
     5941 tcgcgatcgc gcggtcgaca tgtgctggtt cgcgtcggcg ccggaccgct atcacgacta
     6001 cgacggtcac gactgcagct cgtgcagcgg cgaccccggc cagggccagg gcaggacgaa
     6061 ctgctcgtcg tatgtcgcct acggtccgta ctacgcgggc acgacctact actacaaggg
     6121 tggcacctgg gtcagcggcg acgatatgga tggccacggc aactacgact accagaccgg
     6181 taactgctac ggccgttacg gcggtggttg cggcagcggc tctgcatatt tccgagagaa
     6241 cgcctcgcac gaccactgtg tccgcaacgg tcacgtgatc ggcagctcgt ggtgttcgga
     6301 cgagctttgg aacacgacgc agccgtataa ttgcttttag tgcgtacggc tctcgcgttc
     6361 gtgatgctcg tagcttgcgc tcgctcggaa ccgagtgagc gcgagttgct ttcgggcgcg
     6421 caggatgcga cgcgcacgtt cttcgcactc ggcgagggcg gtgactgcaa gcagctcgca
     6481 ccgatgctgc agcgcccggc gacgtgcgac gacctcgtgc ggcagtttcg cgacacgcac
     6541 gcgcacctct cgcaggtctt gggcgccaag atcgacggcc gcgacaagca catggtgctc
     6601 gttagcgtcg acgcgacggc gcccgagcac gttcatcatt ggatcgtgcg cgcgaagtgg
     6661 acatccagtg gatggaagct cgcgctgtga gggttcgaac gctcgctcct accctcctcg
     6721 cgctcggcgc cctcgtcgcg atctacgtgt tcgcgcgtca cggcgacgaa ccaagtacgc
     6781 cctcgccctc cgccgcgcag aacgtgcacg cgcctgctcc ggccgctgcg ccgccatccg
     6841 cgccgccgcc tgccatcacg gctgcgccgc tgcccgcgtc cggcgacatg ccggtggcgc
     6901 cgcctaaagc gctgacagtc gacgagtacg ccggtcacga gcccaaagcg aagaagccaa
     6961 agatgacgct ccaggaggag ctcgccgaga cgacgaagca catctcggtg atggagaagc
     7021 gagcgacgct gatcgaccag caggtcgccg agctcgagaa ggccggtaag acaaaggaag
     7081 cggcggaaca gcgggtgatc gcggaacgcc tgcgcaagca cgtcgaacag ctgcgcgaag
     7141 ccgtggcggc cgggcgcgca ccgaatgaga cgagcgactc gaaatccggc gacgagcaca
     7201 cgccgtgaag cgcgtcgtcg cgtgcctgct gctcgcagcc ggctgtgacg taaccaccgg
     7261 ccaacagact cacgcgatcg ttggtggcca gctcgacccc gatgacttgt cgattgtcgc
     7321 cgtcgtcgca cgacgtacgc agtgcaacca gccgtcggtc ggcatccagt gcacggcaac
     7381 cgtgatcggc gaacaggtcg cgttaacggc ggcccactgc gttacgcagg acgccgctgg
     7441 cacgctcgag gtgtacatcg gcgcgcacgt cggtgatcca ggtgggcgct tcctcctcgt
     7501 cgacgacgtc atgcgccatc ccgcgttcga tgacgagacg cacgcgtacg acattgcgtt
     7561 gctgcatctc gcggagcgga cgatcgtgcc accggtggat ctgccgttag cgcggctcga
     7621 tgattcatcg gtgggtgcgc gcacgcgcgt ggtgggctat ggcgtgacgc aggctggcgt
     7681 gctcgcggat ggccagcggc gcgagacgac gatgctcgtc agtgcggtcg acgcgcacac
     7741 gtttacggcg acgcccgatc cgggcaactc gtgcggcggc gattctggcg ggccggtgtt
     7801 cgttgccacg cctaccggcg agcagctgct cggcgtgacg tctgctggcg atccgctgtg
     7861 cgcgacgcgt gcggtagcga cccgcgtgga tgtcgcgctc gacttcatcc ggacgtatgt
     7921 gttgtcgcca ccgttgatcg cgcccgcgac gatcgatccc gccgagacgt gcaccgcgat
     7981 gtgctcgacc tcggcggagt gcccggctgc gatggtctgt gacctcggac ggtgtcacct
     8041 cgccggcgcg ctgcccgcga gctatcacga cacctgcacg caggacgcgg actgcgatgg
     8101 aacgtgcgcg cagctctcgc cgaatgagtg ccggtgcgcg atcgcgtgca gcatggtcgg
     8161 tggtccaggt agcccgggtg atgacgacac gtgcaacgcc agcggccgca gctcgtggct
     8221 cgtgctcctc gcgctcgcgt ttatggtgcg caggtcaagg cgccgaacgg cggtctgaag
     8281 cagacgcgca cgtgcttcgc cgcgccgcgt gcgatgagct cgtcggccga cagcgtcgtg
     8341 aagaaccgca ggtacgcgcc gtgctcgagg ggaatcgtga catggagaaa cggatactcc
     8401 tggcggcgta caggcgtctg ctcgttgatc tcggcatcct cggggggcct ctcgttgacc
     8461 ttcggcagca cgaacgtcag gtgctcgcgt tgcatcagct cgatcgtcgg ccagcgcgcg
     8521 tgaccgggaa gctgttcgtc tttgctgcgc agatagaagt cacgcgtgat gcgcttggag
     8581 acgagccccg tcgcatcgat cgtgcgcagg tcgtgcccgt agctcatcca gcctccccaa
     8641 tccgcgagga cggaatcgcc cggctcggcg aacactgaca gcgcgcgcgc gaatgcgctc
     8701 ggttgctcgc gcgtcacggt gaggatgcgc ttgtgcgccg cgggcgccgg cgccgtcgag
     8761 aacaccggga cgggcacgag ctggcgatac gcatggccac ccgcgaacgc taccattgcg
     8821 acgatcgcga gtgtttttga cgaccatgca cgtgccgcga gcaaaccgac gacgacgtag
     8881 aacacgccga cgacaggcgc gaggatccga aagtccgtca tgtagtcgcc gccagtccac
     8941 gcagccatcg cgagctgcgc gatgacgaac gcagcgccca gcaacaacgc gcggtccggc
     9001 ctcgtccgca aaccggacac cagaagcggc agcgtcgcga gcgcggccag gccccacagg
     9061 cccatacgcc gcaggaacat cgacacatac cccggcccga aatcgagcgt cgcgacgccg
     9121 ctcaccttcg cgcgatacgg attggcgacg agctcgccgt aatacagcag acgcgcggcg
     9181 acgagcgcga cgacgagtgg cacgaaccag ttcagcgccc ggcggagctc cgcggtctgg
     9241 cgcgtcgcga ggagatgcgc aaaccagatc gctgcgaaca tcacgccctc gggtcgcgac
     9301 gcagccagtg cgacatgcac gaccgccgtc atgcggattg ccgttggacg ctcgagttga
     9361 agtgcgagcg cggcaagcac gagcgcccca tgcgtcgcgg tctccagccc accggttgcc
     9421 cacacaacgg cgatgcgatc cgtcgcgaac agtgccgcag aaaatgcgac cgccaccagc
     9481 ggtagaccac gccgaccgag gagccacacc aacaaagcga cctgcaccgc tgttgcaacg
     9541 atgccgagca cgttcgcgac cgtgaagaga tcggcatgcg ccgccgcaaa cggcgcgagg
     9601 agcatcacga acaggaagtt cgagaacgcc tcgatccgtt cgccgatgtt gaagacgagc
     9661 ccgtccccgc cgacccagtg gcggacgtag cgaaagctga tgagcgcgtc gtcgcacgtc
     9721 cacgcggtaa cggcggcgag gacgcaaacg acggcgaccg cgacaccagc gatcgtccag
     9781 agcaaagcgc gacgcgacaa cattctcagg ctatcaggat cgcaactgtc ctctgtatca
     9841 ggaggtttcg gggtaggacg ttcccgcaga tcacttgcga cagtgacgta ccctgcgttc
     9901 ggccgaccgt agtacgatga aaacgtgggg tctgccgagg agttcggccc gtatgtcgtg
     9961 cacgagcaaa tcgggctcgg cggcatggct accgtccatc gcgcggagac tcagggcatt
    10021 gccggtttca gcaagcaagt cgcgctgaag cgcatgctgc cgaacgtcgc ggcggacgcg
    10081 aacctcgtga agtcgttcat tcgcgaggcg cggctcgcga gccacctgcg gcacgcgaac
    10141 gtggcgcaaa cctatgacct cggcaaggtc ggcgagacgt acttcatcgc gatggagctc
    10201 gtgccgggtt ggaacctccg cgagatcttg aagcacagcg cgcagacgaa gcggaacatg
    10261 ccgctgccga tcgcgctcaa tatcgtcaac cagatctgcg atgcgctcga ctacgcgcac
    10321 aacctctgcg acgagaccgg ccagccccta ggcatcgtcc atcgcgacgt ctcgccgtcg
    10381 aacatcatcc tcggcgaggg tggcgtcgcg aagctcatcg actttggcat cgcgaaggca
    10441 agcacgtccg gaatgcagac gatgtccgga acgatcaagg gcaagttcag ctacatggcg
    10501 ccggagtatc tgatgggata cctcgactgg cgcggtgacc tgtttgccgt cggtgtgatc
    10561 gcccacgagc tgctgtcgaa caagccgctg ttccagggca aggacgacat ggacacgctc
    10621 taccgcgtga aggacatgcc gatcttgccg ccgtcccggt tcaacccgct ggtgccgccc
    10681 gagatcgaca acatcgtgat gacagcgctc gaacgcgatc cgaatcgccg ctggcagcaa
    10741 gcgagtgcga tgcgctctgc gctgacgacc gagacggcgc gcctctcgct cgtcgcgcag
    10801 aaccaagaga tcgacgtttg gctcgaccag accttcgcgg gtggcgccga cgatctcgag
    10861 gctcccgaga tttcgctcgg cggctcgacg atggagctgt cgaagggctc ggtgcccgac
    10921 gatttcctca acgccgcagc cgactcggtc tcgacgctcg tgcggcccgt cggcagccag
    10981 ccgattcaag cgcaggggac gcctgtcgcg cgcgacagct cgccgacgct cggcgatcgc
    11041 ccgagccggc agatgaacgc gcaaaagtcg cgcgccgagt cgccgcgtcc gccgtcgcgg
    11101 ccgccatccc ggccgagcca gaacgtcgtc cctcgtggcg agttcggaga caccgcgcaa
    11161 aatcgcgact ggggtgacga tccgccgacg cgcgccgacg cctcggccgt cgatcagatc
    11221 gcgcagagct cgctcgctcg ccgagatgat cagcgcacga tgaagggcgc cggaatcgac
    11281 gggctctcgc agcgcacgct cgtctcggcg ccgcccgccg agctcctcgc tcagcagagc
    11341 ttcacagaag aaaccgtcgc caacgccgcg gcgatgcgat cgctcgctga cagcccactc
    11401 gcacgcgacg agagtctcga ggcgccgacg ctcacaaaca cgaagcgcgc cacgaccgat
    11461 gtgaccgatg ccgatggcgt cgccgctgaa tcgcgtcgcg atgagttcgc gcagcacgtc
    11521 gacacgacgc cgcgccgccc aacgccgcgc ccgcaacccg ccgcgtcccc gctcacgaac
    11581 gtcccgaaga tccgcgacga ggcgacgaca acgcaggacg cgccgccgcg ccgcgacagc
    11641 ggccttcgtc cgtcgtcgcc gagtgctccg cccgcgcccg gacctctacg gaccacgctc
    11701 ggcgtcagcg caccaccacc gatcgtgcac agcggcgcgc gcactcgccc gcatattccg
    11761 aaggccagcg ctgaaatcga ggtcggctcg tcggcgatcg tcgcggaggt cagcgtatcc
    11821 gacgtgctcg cggacctcgg tcatcgaccg acggcgcaca cgcctgcgaa gggcgcggcg
    11881 atgccgatgg cgccaaagag cggcggtagc agcttcttgc tgatgcttct ggtcctgatc
    11941 gtcgccggtg gcgcggcagc cgtggtttac ttcgcgctgc cttacttcac ctgatccgca
    12001 gaagcacgaa tgaacaagcg acgtatcgcg ctgatctcga cgggcgggac gatcgagaaa
    12061 acctacgacg agctgtcggg cgtgctcgcg aaccaggtct cggtgctcga cgtcatgctc
    12121 gcatcgctcg agctccgcgg cgtcgaggtc cagcgcgtcg cgctgatgaa caaagacagc
    12181 ctcgagatga cacccgacga tcacgccctg atcgccacaa ccgccgccga catggcgaag
    12241 aagcacgccg gcgtcgtcat cgtgcacggc acagaccgcc tcgccgtctc gggcgatcgc
    12301 atctgcgagc tcggcacgcc cgcatcgccg atcgtgctga ccggcgcgat gcgcccctac
    12361 gagctgcgct ccaccgacgc gctgcaaaac ctcacggagg cgctgctcgc cgtccagatc
    12421 ctgcagcccg gcgtctacgt cgcgatgcac aacaaggtgc tgcagttccc cggcgtcacc
    12481 aaagatcgca acgcgggtac gttcgtcaag gaaggcgcgt gagcgaactc ccgatcgcgc
    12541 tgctccccgc gcttctcggc gcactcgatc gcgccggcgt cggcgtggtc gccatgcagt
    12601 acagcggcga cgcgacatcg aagctctacg cgaacgagtc gctcgcgacg agcctcggct
    12661 acacgccaca agagtggcgc gcgacgccgc tctggcacgt catctcgccg cgccaccgcc
    12721 ccgcgatcaa cgcgctgttc gagaagctcg cgcgcacgcc cgacgagccg atgccgaccg
    12781 cgatcgaagt catgctcgtc caccgcgacg gtcacgagat cgcggccgaa gtcgccgcgg
    12841 gtcgcgtcga agtcgcaggc ggctacgcgt tcgtcttcat cgtgcgcgac atgtcgctcc
    12901 atcgccaaac gcagctctcg ctcctcgagg ccgatcgcct cgcgctcgtc ggcgcgctgg
    12961 cctccggctt cgcacacgaa accaacaacc cgctgacctc cgttctcctg aaccttcgct
    13021 cgctgcgcaa gcaagtgcac gcgcacatgc ccgagcaatc gcagacgacc gccctgcgct
    13081 gcctggacga catcacgacc ggctccgagc gcatcgcctc gaacgtccgc gcgctccaaa
    13141 ccctcgcgac gcgcagcacg acagccacga tcgacctcgc gatggtcgtc tcgtcggccc
    13201 tccgcctcgc cgcgccaacg ctcgagcccc gcgcgcacgt catccgccag atcttcccgg
    13261 tcgcgccggt cctcggcgaa gaatcgcgca tcggccaagc cgtgctcgcg atgctcctct
    13321 tcgcgagctc gggcttcgac accgacgtcg ccgtcacaag caaccgcatc gtggtcgcgg
    13381 tcgaagatcg cgatggcaag gtatgcgtcg aggtcaccga caacggccgc gcgctcaccg
    13441 acgtcgatgc cgaacacgcg ttcgatccgt tcttccgcac gccggtccgt ggtgcaggcg
    13501 tcggcgtggg cctcggcgtc gcgcgctcgg tcgccgtgac tctcggtggc gaagtcgcgc
    13561 tgactcggag agacggcggt ggcgctgttg ttactatgcg gcttccgccc gctccgtgag
    13621 ctcacatttg acctttcacg ttctgcttcg atggaacgcc ctcagttgct ccagcgctcc
    13681 ggctcgcgct cgcgcgcgct agcgggtggc gcagcgacag ggttccggcg acgtgtaacc
    13741 cgacgtgccg ctcgcgcccc gcaccctcga tcgtgactag cgcccgaccg cactggccgc
    13801 cccaccgcgc tgacacggcg acggggccct gtcgcgcagc cgcccgcccg cgcgcgagcg
    13861 cgagacggag cgcgcgatcg gcggaggccg catcggcgga agcgggcgct acggcgggtc
    13921 gcgcatcacg aggaagatcg cgtgcgcggt ggcgaccggc gtctttcgat ccgtctgcca
    13981 ggcctcgacg cgcacgttcg cgacgcgacg accgtgacgc gtgacaatgc tgcgcgcgtg
    14041 cgtgtcgacg ggcgcgccgg agcgcaggta gtcgacggtg atcgtgatcg tcttggggag
    14101 gacgatcgtc tcggcgcgcc acagcagctc gaagatcgcc gacgactcga ggagcgcgcc
    14161 gagcgtccca ccgtggagcg cggggagcgc gggattgccg atgagctcgg gcgagaactt
    14221 catcgtcgtg atcagctctt caccatcgag gcgcgcggag agaccgagga accgcgagta
    14281 cggcacgatg tcgaagaggg cctggcagtc gccagatcgc tttgcctctt cgaggcgttc
    14341 aaccaggctc atggcgcgtc cttcttggag ccggatgccg ggccggttgt gccgaggccg
    14401 gtgccgccga cgccgcccgc gggcttcgtg ccgagcatga acgtgccgac ggagctggcg
    14461 attggatcgg cttcgtcgtc gtcgtggtac gcgatggcgc gcgtgaacgc gacgttgcgc
    14521 gtgatgcggt agcaggtcgc tttggcgcgg attgcgcggt ccacgtcgcc ggtgcggagg
    14581 taatcgatgc gcagatcgag cgtcgcgatt ggctgccact cggtgagcgc ggcgaagacc
    14641 gcggcgccgg agcacgcgtc gaggagcgcg gtgatcgcac cgccgtggag gacgcccgtg
    14701 tcggggttgc cgacgagctt ggtgtcgtag ggcagctcga aaatcgcttc ggcgcggcga
    14761 aactcgacga ctttgatgcc gagggccttg ttgtgcggaa tgccgtcgac gaacccgcgt
    14821 gacactcgaa gcggcttgtc gtcggaagcc atgtggcgac attatagggc gctcatggct
    14881 ggccgcgcgc ccatcccgac ctggtacttc gcgctcgtcg tcgttcggcg cgggcaacgg
    14941 tttctgctca cgcaggagcg caagtacggc tcctcgtggt cgattcccgg cgggcgcgtc
    15001 gagccgggcg agtcgctcgt gcgcgcggcg atccgcgagg tctacgagga gacgggtgtg
    15061 cccgtgcgcg tggatgggat cttgcgcgtc gaacattcgg cggcggagtc gacggtgcgc
    15121 atgcgcgtgc tgtttaccgg tacgccgctc gacgatacgc cgccgaagac gaccgccgat
    15181 gacgagtcgc taggtgctgc gtggttgacc ctcgacgaga ttcggaagct gccgctgcgc
    15241 ggcgcggagc tgcgcgcgtt gctcgagagc gtggagtcgg ggcgacaggt gtttccgctc
    15301 gatctcctcg gcgattcaga aatgtcgatc tagatgctgc aacggatggg ctgggacacc
    15361 gactgggatg atgcccggcg agccgtcgat ccagactgcg tgctgcatcc cgtgcgcgtg
    15421 gtcgcggagc accgcggcgc atatcacgcg acgaatggcg acggcgtcgg atggatcgag
    15481 ctgcccgggc gcacgtatca ccgcgcgcac gacaagcgcg agctgcccac cgtcggcgat
    15541 tgggtgatgg tcgagcgctg gcccgaggca ctcgcgagta atggcgcggc ggtgattcgc
    15601 cacctcctgc cccgtcgcgg cttgctcgtg cgcaaagcag ccggcgaagc gacggtgcca
    15661 cagccggtgg ccgcgaacgt cgacatcggc atcgtgatga cgagcgcgaa cagcgacgta
    15721 tcgcccgcgc gcatcgagcg ctatctgtcg ctgctcagcg acggaggcat cacgcccgcg
    15781 ctgatgctct cgaagatcga tctcgtcgcc gatgctggtc ctctgctcga cgagctgcgc
    15841 gcgatcgcac cgggccacac cgtgctcgca gtgtccaagg tgaccggcgc cggcatcgac
    15901 gaagcgcgcg cgctggttgg gccgggccgc accgcagttc tgctcggcag ctcgggcgtc
    15961 ggcaaatcgt cgctgctcaa tgcgctcctc ggcgagggga cgcaactcac gcaaccgatc
    16021 cgcgacgacg atcgcggccg ccacacgacg acgcgccgcg agctgttcat caccgagtac
    16081 ggcctctgga tcgacacgcc cggcatgcgc gagctcgcgc agttcatcga cgaggacgac
    16141 cccgagctcg tcttcgacga catcgatgcg ctcgccgagc agtgcaagtt ccgcgactgc
    16201 caacaccggg ccgagcctgg gtgcgccgtg cgcgaccaca tcgatccccg tcggctcgcg
    16261 agcttccaca agctcctcga ggaacgtgcg accggcgcgg agaagcagaa tgtcgcgcgg
    16321 cgtctcgcag aaacccgcag ggcgaaagcc aataagtacg ccccgcgacc cggcaagaag
    16381 gaccaagact gaaagttttt cggacgccgc gagatgctat aagccgcgct cgtgatcgcg
    16441 ttctcgggtg tgaccaagca gtacggcggg cagattctgt tcgtcgacgc atcgtttcag
    16501 atcaatcccg gcgagaaggt cgggctcgtc ggtccgaacg gtgcgggtaa atcgaccgtc
    16561 ttccgcctca tcgtcggcga agaaactccc gacgacggca acgtcgagaa accgcgcaag
    16621 ctaacggtcg gctacttccg ccaggacgtc ggtgagctgc gcggccgcac gatcctcgcg
    16681 gagacgtgcg caggcgcggg ggaggtcgct gagctggcgg acgagctcgc gaggctcacg
    16741 accgcgctcg agaccgaccc aaccgacgag gtcgtcacac gctacggcga agttcagtct
    16801 cgctaccagg atctcggcgg ctacgagctc gaggcgcgcg cgcaagaaat cctcaacggc
    16861 ctcggctttc atcaagagca gatgtctgcc gacgtcggca cgctctcggg cggctggaag
    16921 atgcgcgtcg cactcgcgca gatcctgctc gcgaagccag agctgttgct gctcgacgag
    16981 ccgacgaact acctcgacat cgagtcgatc ctctggctcg agacgtttct gcgcgactac
    17041 gcgggcaccg tggtgatgac gtgccacgac aaagagatca tgaaccgcgt cgtcaagaag
    17101 atcgtcgaga tcgacggcgg cgcgcttcgc gcatacggcg gcaactacga cttctacgag
    17161 ggcgcgcaca agatcgaagc ggagcgccgc gaagcggaat acgcgcgcca gcaagcgatg
    17221 ctcgcgaagg agagccgctt catcgagcgc ttcaagaccc acgtcgcgaa ggcagcgcag
    17281 gtacagagtc gcctcaagaa gctcgacaag atcgagaaga tcgccgagcc aaagcgcttc
    17341 atcgagaaga gcttcgattt caagacgccc tctcgctcgg gcgacgatgt cgtccgcgtc
    17401 gaagcgctcc gcaaggcgta cggcacgcgc gtcgttcacg acaacctgac gctcacggtc
    17461 cggcggcgcg agcgctgggc tgtcatgggc gagaacggcg cgggcaaatc aacgctgctc
    17521 aagatgatcg ccggcgcgct tccgcccgat gcaggcgtcg ccgcgatcgg cgcgtccgtg
    17581 cagatgggct actacgcgca gcacgtgatg gacctgctca cgagtgatcg caccgtgctc
    17641 gaggagctgc aggagcacgc accgtcggcg aatcaaggca cgctgcggag cctgctcggt
    17701 gcgttcggct tccacgacga cgacgtcttc aagccgatca gcgtgctgtc cggtggcgag
    17761 aaggcgcgcg tcgcgctgtc gaagctgctc tacgacgcgc cgaacctgct cgtcctcgac
    17821 gagccgacga accacctcga catcgcgacg aagcgctcgc tcgtcaaggc gctcgccgat
    17881 tacgaaggca cgctgatctt cgtctcgcac gatcgccagt tcctctccgc cctcgcgacc
    17941 cgtgtgctcg agctgtcacc gcaaggcgct cgcgtctacg gcggcagcta cgacgaatac
    18001 gtcgcgtcga ccggccgcga agcgcctggc atgcgcgcgt agcaagtaag aagtacgacg
    18061 acgacgctgg cgacgccgaa cgcgatcgcg ctcgccccgc gtgttaccct cgacacgtgg
    18121 cagcagacga cgacgatcca cgccgccaag gcggggcgcc gcctccgcaa aagaagacgc
    18181 agttcggcat ggtgattccg acgccgtccg gtgcgcccca agctccgcag gcgccgcgcg
    18241 ttccggccgc gacaccgcag ccacccgcaa cgaacaagac gacgttcggc gtgccgtctc
    18301 cgatggcgcg tccaccggcg caacgcgtga cgcccgcgcc gatgcccgtg cagcgcgtaa
    18361 cgccagcgac aacaccggca cagccgcaac cgcaaccgcc tccgtcgaac gcgacgaccg
    18421 gcaccgcgca cgggcaggcg accgaccagg atctcccgca cgacgtgatc gcgtcgggca
    18481 tggggctcga gcccggcggc cgcgttcacc agtacgagct gattcgcgag ctcggccgcg
    18541 gcggcatggg catggtgtgg gccgcccgcg ataccaagct cggtcgccgc gtggcgatca
    18601 aattcctgct cgatgcatcg cgctcggtcg cggatcgctt cctcaccgag gcgcgcgcga
    18661 ccgcgcagtg caatcacgaa aacatcgtca tcatccacga agtcgacgag atcgagtcga
    18721 tgccgtacat ggtcctcgaa ttcctcgagg gccaaccgct gcgcggcgtc atgggcacgt
    18781 acggaagcgg catgcgcctc gcgccgacgc gtgtcgtcga gctgatgctc cccgtcgcac
    18841 gcgcactggc tcgcgcgcac gagctcggaa tcgtccatcg cgacctcaag ccggagaacg
    18901 tgttcgtcac gaacgccggc caagtgaagg tgctcgactt cggtatcgcg aaggcggtgg
    18961 cgcgtcgcga tccgcaggcg cagcagcgct cggtctacgg cgacctgcac gcgcaaggcg
    19021 cgaatctgaa catgacgcgc gagggcgcgc tgatcggaac gctgccgtac atgtcgcccg
    19081 agcagatggg catcggcgag gtcgatcacc gctccgacat ctgggcgttc ggcatcatgc
    19141 tgtacgagat gctggcgggc cggcatccgg tctatccgct gacctccgaa gcgctgatca
    19201 cgaacgccgt ctccgaggac gcgatgccgt cggtgcgctc ggacgtcccc gatgtccccg
    19261 acgcactcgc gtggatcgtc gacagctgcc tgcgcaagcg cgtggtcgag cgtatgccgc
    19321 gcgccacgga cctcgtaaag aagctcgaag agctgatgcc gggtcgtgcg ggccgccagc
    19381 tcgccgaagg tgagagtccg tatcccggac tcaccgcatt ccaggaagcc gatgcggatc
    19441 gcttcttcgg ccgcacgcgc gaggtcgcac gcatgacggc acgcattcgc gaattcccga
    19501 tcacgggaat cgtcggaccg tcgggcgccg gcaagtcatc gttcatccgc gctggcgtcg
    19561 gtcccgccct caaagcgagt ggcgagcact gggaagtcgt cacgctgcgc cccggccgcc
    19621 agccgctcgc cgcactcgcg agcatcgtgc agcgactgac gacacgctcc ggcgtcgacg
    19681 tgcaaacgca ggtcgccgat caccacacgc tgctccagcg gctgcgcggc gagcccgggt
    19741 tcgtcgggac gctgctccgc aatcgcgcgc gccagaccgg cacgcagatc ctgctgttcg
    19801 tcgatcaatt cgaagagctc tacacgctcg ttcccgatcc gaatgaacgc cgcgcgttca
    19861 ctgcagcgct aacgggcgtc gcggacgata cggcggcgcc gctgcgcgtc gtcgtctcga
    19921 tgcgctccga tttcctcgat cgcatcgccg aggaccagcg cttcatggag gagctctcgc
    19981 gcgggctcgt gttcctccag ccgccggacc gcgccggcct gcgcgaagcg ctcgtcgcgc
    20041 ccatcgagat ggtcggccat cgcttcgagt ccgcggcgat ggtcgaggac atgctcgatg
    20101 cgctcgtcaa cacgccaggc gccctgcccc tcctccagtt cgccgcggca aagctgtggg
    20161 atgcgcgcga tcgccagcga cgtctgctga cggtcgccag ctacaactcg atcggcggca
    20221 tctcaggcgc tctcgcgacg cacgccgacg acatcgtcgc gcagatgaac gatcgcatgc
    20281 agcggctgac gcaaaagatc ttccgccgcc tcgtcacgcc cgagcgcacg cgcgcgatcg
    20341 tcgagctcgc cgacctctat cagctcgcgc cggaccaagc ggaggtcagc cgcacgatcg
    20401 accagctcgt cagcgcgcgc ctgctcatgg tgcaaacgcg aggcgacacc ggcagtagcg
    20461 gcggtggctc cgtcgagatc gtgcacgagt cgctcatcga tcgctggcca acgctccggc
    20521 gctggctcga cgaagatcaa gaagacgccg cgttcctctc ccagctgtct tccgccgcca
    20581 aacagtggga agccaaaggc cgcagtcgcg gtctgctctg gacgggtgac gcgatggaag
    20641 aggcgcgccg gtggtccgcg caacgcccgc gcgagctgcc cgcacgcgag caagccttcc
    20701 tcgatgccgt gttctcgcac gcacaacgcg gcaaacgcgc caaacgcgcg ctgctcatcg
    20761 cgacattcgc gctcctcggc atcgtcgcgg cgggcgcctc cgtcgctgcc gtcaaaatcc
    20821 gcgactccga gcaacaagcc aagacggcgc tctcgcagat ccagcagaag gaagccgaac
    20881 gcgatcgcgc cgaagccgcg caaaaggccg ccgaaaccga aaaggcccgc gccgaagaag
    20941 ccaagcagaa agccgagctc gacaagcaaa aggccgagct cgagaagcac aaagccgagc
    21001 aagacaagct cgccgccgaa gccatgagcc gcgaacagct ccaagcgaaa aacctcgagc
    21061 tgcagaagac gctcgacgag gtccaaaccg ccaaagcgac cgccgagaag gcgaaagaga
    21121 tcgcggtcaa agagaagcag aacgccgagc gcgcacgcga cgaagcaaac gccgcgaagg
    21181 cgaagatcca ggccgagcta gaccgccagc gccaagagaa cgcgaagctg tcgcacaaac
    21241 tgaagtagcc gccgctggtt tcgtgctccg caaattgttc cgctgccgct cggcttcgcg
    21301 gatcgcgcgc tgtgtctcgc gctcgcgcgc gagcgggctg cttcgcggca acggacggag
    21361 ggaacgcaag ggagccagca gagcaaggat cgagtaacga caagcgatcg acacgcgatc
    21421 gatcggcgag aagcgggcgc agcgatggcg acggcgacta gaagcggccg ctgagcgtca
    21481 gcacaccacc gccatcgtgc ggcacgtagt cgaggcgcgc ggtgccgagc gggccttgct
    21541 gcttctcggg ctgctcgtag acggtctggc cgcgattcat cacgagcagc acgccaccag
    21601 cgaccgcgcc ggcggcaccg accgagatga tcgcgatgcc gatgttgccg agggtcttcg
    21661 cgtcgtcgcg ctgatcgcgg agcgattggt tcgtattgag gtcgcagcca gtcgtttggc
    21721 agttcgacga gacttgcttg tcgaacgtgc tcatgttcga gaacgcggtg gcttcgatca
    21781 cgccgccgaa gcccgcgacg acgaggccgc cgccgaacac gacccatggg atccatgtcg
    21841 gccagcgatg gacgatcttt gcggctttgg cgagcgggac gaggttcgtc tcgaccgtct
    21901 cggtgttgcc gcccaccacg acgacctcga tgttctgcgg caggaagcct tccttggtcg
    21961 tgacgactcc gtgctggccg ggcttcacgc gacgtttctc gctgcctggg cattttgcga
    22021 tcagcgtctc gccgtcgagc gtgaccttcg cgccgtcttg cttgcagctg atttcgacct
    22081 cggcgatctg gttcgcgagc agcttctggt acgcgagcgc ttcggtgtag acggcttctt
    22141 cgagcggcgc cgcgccgtac ttgagcgcga gctgcaggtt gtcggatgcg ggcacgggct
    22201 gatcgagttg gatcaggcag cgcacgatgt tgaagcggat cgcggggtgg tcccactgcg
    22261 cgaccgcggc ttggtacgcc ttgagcgctt cgggatagtt ctgcttgagg aaaagggcgt
    22321 tgccttcttc gagcttcttc tgcgcttcgg ccttctgcgc ttcgctgacg ccttgggccc
    22381 agggttggtc ggcgtgcgcg atgttcgcgg tcgcgaccca cagccccaca accagacata
    22441 gcgttcgcag cttcatggcg ggttcccgga gctaggttct cacagcgccg gggcgcgtcg
    22501 actagtcgtc ggaagatttc ttcttcttct tcgttttctt cttcggcttg tcttcggtct
    22561 tcggcttcgg cggcggctcg tcctgcttga cggcgggcgc cgcgatcttc ttgaagctcg
    22621 cgccttggcc cggctcgtcg atcgcgagcg actcctgcag cgagatgagc ttccagtcgc
    22681 tgcccgcctt ttcgtagacg ccgaacaggc gcagcggcag gggctgatct tcgttgtcct
    22741 cgaaccggac gaccggtgcg ctgacccacg cgatctgtcc gtcgggcgtc gtgcctgcga
    22801 gcgtgtcgcc cgctgatgcg tagcggacgt tcgctttcgt gcgcttcttc cagagctttg
    22861 cgatcgccgt cttgccgcgc gtgatttctt cggcggctgg accgatcacg accgcatcgc
    22921 cgcgcttgct gagatcatcg ccccacaccg ttggatcgcc gaagcctcgc ttgaagcgat
    22981 cggcggcggc ttccgctgca tcgggcacgc tgcccggcgc ttccatcgcg ggcggcacga
    23041 cagcgtcctt cgcgagcgac gcacgcaccg atttcatcga cggcgtcacg gccacggatg
    23101 cggcggcgac gacccagaag tcatcggcgt tggacagcac gactgtcact gccatcgtct
    23161 cgccctggac atcgatggtg tcgacggccc acgcggagag gccaccttgc gacgcgacga
    23221 ccgagaggtt gcccgacttc accgggaact tcttgtcctt ctctgcgtcg aggatttcgc
    23281 gcagcgtgac gagcgcgtcg gcgcgcgtag cgagcgcgtc tttcttgcgt gggccgagca
    23341 cgtagaccgg atcggcgacg agcgccatca ggccgtcggt gttcgagtga ccgatcgtcg
    23401 cgtagatctc ggtgacgaga ctcttcgcgt ctttctcggc tttgcgttcg ctgaccgtcg
    23461 gagtcttcgg cttctcgacg tcggtggtcc cgccgccgcc gccgcacgca gcgaccagcg
    23521 cgacgacgag cgctagcgct ccttggctgc gactcgcttg gtgtgcgcgt agccgactgg
    23581 cttcacttcg gccggcatcg tcacgtacgg cgacgtgtga tcgtcgttcg ccggcactgc
    23641 ttcggtgcgc acggtgtcct ctccgacgga gcccgtgcct cgagggctgc ggcgcggcgg
    23701 tgagtacggc gtgtacggct ggagctcgct cggcttcgcg gtggcctgca gcgtcggcac
    23761 cggaatgacc gtcttgcgct cggcgatcgg cgcaactggc ttcggcatcg tgcgaaccgg
    23821 tggctccgtc ttcgcgcgga ccgccggaat gctcggcgac gtcttgcgcc tcggtgcggt
    23881 cacgctcgtg gcgatcgcaa ggcccggctt tgccgggtcg cgcagcactc ccgaagggag
    23941 tgcggagtcg atcgacgccg ctgcttgctc gacttcttcg gccgccgccc gcgccttcgc
    24001 catcatgatg tcccattccc attcatcctc ctcgaccggt ggctcctcgg caacctttgg
    24061 cggcaggacg gcgacgggcg gagcgacagg cgcgaccaca ggggcgatca cgatgggccc
    24121 agtcggcgga aggccgggca gcttcgcggc gggtgcaacg atttttgccg cgaggggcac
    24181 cggcggcggc gcgaccggaa gcttgtgcaa actcgccgga gcggcaacta gcttggccgg
    24241 cttcttcttc ttcggaacac cgatgtgctc gagctccatt gcggccagat cgtcgtcggt
    24301 ccagcccacg atcttgcctc ccaagccgtc gtcgagcacg gcaggtcgcg tggatagcac
    24361 gcgatcgtcg cccgtcagga tgaggccgta ccggtcgagc ttggcgtagc cgaagcggcg
    24421 aaggaagttg tcgagggcgc ccatgcgggc ttccgtgtgc agccagtggg ccagcgcgaa
    24481 cacagcgcaa ccttgcagac cctcggaggt gctccggacg gtcgagtcgc ctggggtgtg
    24541 gggtcccgac ccggctggtg cctcggcccc cagcgcgatt tttctgtaac taattcacgc
    24601 gctcggccac cgcgttagcg gccttgatac ttcggcggcc ggccttcggc gaacgcggcg
    24661 agaccctcgt cacgatcttc ggagtacagc gttcgctcgt aacactcgcg ctcgatcgtc
    24721 aggccttctt ccatcggctt gccgaatccg agctcgatcg cagacttcgc gctcatcacc
    24781 gagatcggtg cgcacgtcgc gagctcggcg agaagcgcgt cgccggcagc acgcaacctg
    24841 tcgcgcggaa cgacttgatg cacgagaccg atctcgagtg cgcgccctgc gtggatgcga
    24901 cggccgagca tgatcagctc cttcgcacgc gcttcaccga tgagccgcgg caaacgctgc
    24961 gtaccacccg cgcccggcat gatgccgagc ttcacctcgg tgagcccgac ctcggcattc
    25021 tcgacgatga tgcggaagtc gcacgccatc gcgatctcga gaccacctcc gtacgccgag
    25081 ccgttcatga ggcacacggt cggctgcttg atcgcgcccc acccttcgat cgcattggcg
    25141 atcgcgttga tgtacggccc agcctccgcc gcagggatcc cctttcgctc tttgagatcc
    25201 gcgcccgcgc agaatgcttt gtcaccggcg ccggtcagca cgacgatgcg aatctcgtcg
    25261 tgacgctgct cgaggtcgct cgcgatttcc tgcaaccgca cgttcacgtc gcgcgacaac
    25321 gcgttgcgaa cctcggggcg gttgagcgtc acccacagga cgccctgggc ttcttcgacg
    25381 aggatgtcgg acatgcgccg acgctatcag gagccgagct tctctgcttg gcgccgcgcg
    25441 cgtgcttgct tgccgagatg cgccttgagg tatttcgacg gcagctcgtg accgacgaac
    25501 gtcgatgcgg tgcgcgacgc ctcggtgagc ttgtcgagat cgatgccggt cttcacaccc
    25561 atcgagtgca gcatcgcgac gacatcttcg gtcgcaagat tgcccgatgc acccggcgcg
    25621 tagggacaac cgccgaggcc gcccgcagac gcatcgatcg tcgtgatgcc catctgcatc
    25681 gagacgaggc agttcgcgag cgcggtgcct tgcgtgtcgt ggaagtggac cgccagcgaa
    25741 tccgcgggat gtttggcgag cacgcggccg atcacgtctt cggtttgctg cgggttcgcg
    25801 acgccgatcg tgtcgccgag cgacacctgg tagacgccca tatcgcgcag cttgccgacg
    25861 agctcgacgg tcttctccgg atcgacatcg ccctcgtacg ggcacccgaa cacggtcgag
    25921 acgtacgcac gcaccggcac gttcgcagcg cgcgcggccg ggatgacttc atcgaacgcg
    25981 acgagcgtgt cggcgatcgt cttgttgacg ttcttcttgt tgtgcgtctc gctcgccgag
    26041 aggaagacgg cgacgacctt catgcccgac gcgagcgcgg tatccaggcc gcgacggttc
    26101 ggcacgagcg cgctcatcag cacgccgggc ggacgctgca cgccgcgcgc gacctccgcg
    26161 gcatccgcga gctgcgggat ccacttcggc gacacgaagc tcgtgatctc gatcgacttg
    26221 atccccgagg cgacgagcgc gtcgatgaag cggatcttgt ccgcggtcgg aacgttgcgc
    26281 gcttcgttct gcaaaccatc gcgcgggccg acctcgtaga tggtcacctg agcgggcagc
    26341 ttcacagagc gcctccgtac atgccgaaac catagtcccg agcgagcttg tcgaccagcg
    26401 gaggcgtgag gtcggcgagc gtatcgatga ggtggtgggc gccggactgg tcccagcccc
    26461 cgaaattgcc agctcgtacc gcgatcacga ggcacccagc cgatcggcca gcctcgatcc
    26521 ccgcggtcga atcttcgacg acaacacagt gtttcgcgtc gaggccgagc gcgtccagcg
    26581 ccttctggta gccgtcggga tggggcttgc tgcggccgac atcctccgcc gccacgatca
    26641 cctcgaatgc cgccatctcg ccgatcagcg gcagcacttg ggtgacctcc acccgcgagc
    26701 tgcccgtcac cagcgcgcgc ggataggtcc tcgtccaggt cagcgtctct cgcgcgtgcg
    26761 gcatgatcgt cacgcccgtc tcggcgaaca cctcttcgcg caacatggcg gtccgcgcga
    26821 tcagctcgtt gcggttccac tcgatctgcg gatagcgctc cttgaggcga tcgtagatcg
    26881 cgacccacga gcggccgatc acgtagtcgc ggtccgactg ctcgatctcg acgccctgcc
    26941 cgcggctgag cgcgcgcgcc atcgcctcac cgctctcgcg ctcggagtcg acgagcgtgc
    27001 cgtcgaggtc aaacaaaaag ccgcggatca ctccgcggcc ttataccact agggtcgctg
    27061 tgaaaattag aagtcgaccc agttgccgtg gtcgcacgcc cagttgccca tgtgcgcgtc
    27121 gtcgacgaac tggccgaacg cgacgcacat ctcgaagccg aacggcagga cgcgaccttc
    27181 cgtgttgttc caggtgcacg agacgtcgac cttgtcgccc gggttcagga tcagcgggtc
    27241 ggctgggtcg cggcggttct cgggcgggtg gaacgtccac gatggatccc agtcgtggat
    27301 gtcgaacatg cgcgtcttct cgcccgactt ggtcacgtcg atcgagatct ctttgcccca
    27361 gcgatgcatg tgcggcgcga agtaccagag cttcatctgg cgatcgatcg tgcagtgcat
    27421 ctcctgcgtc gtcatgccgg gctgcacttc gaggccggtg ttgacgatcg ccgtgttgcc
    27481 cgacgggatc cagtcacccg catccgcgaa cgcgatgttg acggccgact ggcccttcag
    27541 cttctcgtcg ctggcgttga ggtagtgatg gttcatgacg atctgcgcgc ccggcggaat
    27601 ccggtacacg agattgcccg gcgccatgtt cttttccttg ttgccgccgt tgccggcgag
    27661 gaagcggaac gtcgccatgt cgttatcggt gcagatgcgc tgcgtgccgg gcggctgctt
    27721 gtcgagcgtg tagaacagga tcacgtggtg acccggcggc tcgctctgga agccttgcgt
    27781 ccagcgcacg tcggtctgct tgtcgaagac agcgtcggtc cacgtgcaga tctcgtagtc
    27841 ggttcccggc tcgacatctt cgaagatcgg cgagacgacc tggatgccgt tagccggcgg
    27901 agcttcgagc tcggcggcca gctccggcag catctccggt cccgaattgt cggctccaca
    27961 accgagcagc gataccccga gcacaacgat gagcttcttc attcccaacg ctccttagtt
    28021 aagacaaacc gacggcgtgc agccgcactg gttgttcccg ttgcaagtgc actccgcgcc
    28081 tgtaccgcac gcgtttgtag atcccatcga gcacgtcttc gtgcagaagt gcgtgtcctt
    28141 gtcgccgagg ctcgagcaga gcggcgcgct catcgtcccg ttgcagtccg agagcgttgc
    28201 gcagaactta ccgatgccga gctcgttgcc cggatcgcca gggtggccgc acagcgagta
    28261 ataggcgtcg accgccgcgt ccatcggcgt agaggacgag tcgtctccgc cgcacgcagc
    28321 gacggtaagc atgatgatga gcacagcggc gcgcatgagc ttccctcgct tagcgctgcc
    28381 gctatagccc gcatcgttgt gcacgctcaa gcacaaccta gcgaatgttg gggcttgtaa
    28441 ccgaggctgc aaagccgtgc acagaccgtg tgtcatgctc gccaagatgc tccgcgttcg
    28501 cgtcgttctc gccctcgctc tgctaactcc ggccgcctgc gcaaaccgcg caaagcagtc
    28561 ggtctccctc tatgaatcgg gggattacgc gggtgctgcc cgcgccgccg acgaaggtct
    28621 cgcgcaacat cccgacgacg acggcctatg ggccatgaag gttcgtgccg cgctcgcgct
    28681 cggtgacgcg gacgcgatgg cgaaggccta cgccggatac atggcgcacc gcggcgaaga
    28741 cgacaaagag ctgctgcgag atctctcgca agcaacgatc gcgcaggcgc tcgcctctcc
    28801 ctccgtgaag ctgaagatcg aagcgatcga agccgtcgcc gcgctcgagc tgcacgacct
    28861 cgccgacgcg gtcgccgagc gcatggacga tcaagatgat cgcgtcgcgg ctgccgcagc
    28921 ggtcgcggtg ctcaaaggct ttccggaagc gccgcgcatc gccgacgaga tgttgcacag
    28981 cgatgtcagc gaagcgcgcc gcatcgtcgt cgatggcatc ggcaagaaag tcggcaagct
    29041 cgcgctctcc gatctcgaga aagccgccgc cgaccgcgat cacaacgtgc gtcgcgcagc
    29101 gatctattgg ctcggtgcgc tcaaagacaa agacgcggtc gaagtcctga cgaagcgcat
    29161 gaaggatccc gacgaaggcg tgcgtgcagc cgccgcgcaa gcgctcgcga agatcggcat
    29221 cggccatctc gacgcgtacg ccaagcaagc actcgccgat cgcgcgctat cggtccgcat
    29281 ggccggcgtc gatctgctcg ccgccgcaaa gcgcgacgcc gatctcatcg cgctcaccga
    29341 cgacaaagat ccgatggtcg ctctctccgc cgcgatcgcc gtgaagtcct cgcgccccga
    29401 gctggcagcc aaagcgatcg atcgcgcgat caccgccgag gagtggacgg tgcgtgcagg
    29461 cgccgcgaac atgctcggca tggcagtcga caaagccacc gccgcgtcct acgcgcaaaa
    29521 gctcgtcaac gataccgccg tcggagttcg cctcgccgcc gcgcgcgtcc tcctccacca
    29581 aggcgatcgc acgaccgcca cgccgatctt ctccgccgcc ctcggagacg ccgacttcgg
    29641 cctccaagcg gcgaccgacc tcgcgagcat caacgacaaa gccggcctcg acgccctcgc
    29701 cgcagcgacg cgcgacacca cgaaaactcc cgagcagcgc gccgcggccg cgtctgccca
    29761 tcggtcggcc catcgcgtaa ctccaggcct cgtcgctgcg ctcgccgacc cgaacggcct
    29821 cgtccgcatc gaagccgcct cgacgctggg cgccctcgca aagtcgaagt aactcctaac
    29881 agttggctac cccgatgagc cccggactat ggggcttcgt ctggtacatt cgggatccac
    29941 cgtgcgtcgc tgggtcccta gccgctgctt tctcgcgttc gcgttcgcgt tcgcgtacgc
    30001 gctcgcgctc gcggcctgcc attccaagcc cgccacgccg accacgaccg cgaacgcacg
    30061 cgtgacgctc gcggtccttc cggcggaaag cgacgcattc cccaaagccg cacgcgcggt
    30121 caccgaatcg ctcgagcgcg cgagcgtctc cggcatcgat gacaagaaca tctcgaaagt
    30181 ctcgctcgag gtcgtgcagc tctcgatcga gtgcgtcgag ccgacgcccg cttgctacaa
    30241 agcagttggc cgctcgctgt ctgcaaaccg cctcctcttc gcgcaaatct cggccgaaaa
    30301 gaaaaaggcc gtcaaagtca gcgtgacgct cttcgacgtc gagtcgcaga gcccgcgaac
    30361 cgcagagaag gtcttcccga acgagaaggc agcaaccgac ggcatccagg atctcgtcgc
    30421 ggaggcaacg cgatgagcac gaagaagcga accgacgaaa cgccgccctc cgacgtcgtc
    30481 gagatggacg cgatggacga ggccgaatgg gagaaggacc agcacacgcc gatggtccaa
    30541 gactccgagc tgcaaaagct cgtcaaggaa agcgcagaca cgccggccaa gggcgtcgcc
    30601 acaaaaccgt ccggctcggg caagcccgcg accgggaccg cgaagaccgt caagcgcgcg
    30661 cccacgagcg caggaaccgc atcgtcgacg accaccgcgg ccgcgcccac gacgcccgcg
    30721 cccacgacga ccgcgcccgc gagcgcgccg acgaaatcag acgcagcgcc gagtggtcgc
    30781 gcgaaacgtc cgacaaagag cgacgcaaaa tcgaagcccg catcgagcgc accgcccgca
    30841 tcccccgcat cccccgcatc ccccgcatcg ccggcgccac ccgcatcgcc cgcggccgcc
    30901 gcccctgcgc ccgccgcacc tgccgcacct gccgacgacg acgcatggtt cgagctgacc
    30961 gatggcgacg acgcgccaag cccacccaag gtcgagaccg gccctgtcgg cgcgaaaccc
    31021 ggctcgcgaa cgatcacgac gaacaccgtg tcgcgcagtg gcgcgaccaa acccgacgca
    31081 tcgaagtcgg cacccgcaag cgcgtcgcca acgagcgcta agcccacgc
//
LOCUS       DATLBZ010000507         9003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_663290, whole genome shotgun sequence.
ACCESSION   DATLBZ010000507 DATLBZ010000000
VERSION     DATLBZ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663290"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2078)
                     /locus_tag="VMZ53_25665"
     CDS             complement(<1..2078)
                     /locus_tag="VMZ53_25665"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HUS31925.1"
                     /translation="MRWTLALALVVACADPPKKPIAGTITRSADARGLPRMLDVRDAP
                     VASSARDHVLHFASAWRVSPLAVPVLDEVGTLNVPGGTITRVRQTIDGLPVWGGELRV
                     LVQDSELRAITGTLVGRDRARSWRVIDDRAMAIARAARGAHVDQALAREVWYPSGDAL
                     IAAWVVDAYTSDPGSAHGDAMRTIIAGDDGRVLERYSLSADAFTYKVFAEPTGEKHPL
                     DGPQVDATPHPSGSPNGFYPAYLAGPNLVSVDSLSASGDPWLPPGATETSGNNVDAYI
                     DVSAPNGLTQGDFRATVSSAGAFSYVYDLELPPLVTAEQQNASITSLFYGINWLHDFW
                     YDAGFTEAAGNAQKSNYGRGGIEGDAILAEAQDNALGGSRNNANMNTPDDGMSPRMQV
                     YVWSGKDNRVLDLSSGDKPEFGVAVFGPKQFDVQGAVVVGQDGAGVNVTDGCEPYTNN
                     IDGKLVAVDRGNCTFKRKALNAQNANAVGVLIVNNQTSTTPPPLGDDSQLANTTITIP
                     TVSVTDVAGAALKAAIAKGTVTATLHREVGTDLDGTLDSTLIAHEFGHYLHHRLTLCG
                     NQMCRALSEGWGDFSALMLTSRPGDNLDRAYPFGIYVTQGYSNDPAYYGIRRAPYSAS
                     FSINALSFRHMASGEAPPSGHPFLVMNSNSEVHNAGEIWASALWQVYAALQKSSTDFL
                     ETRAKMARYVV"
     gene            complement(2091..3308)
                     /locus_tag="VMZ53_25670"
     CDS             complement(2091..3308)
                     /locus_tag="VMZ53_25670"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HUS31926.1"
                     /translation="MRFALALLILVTTAPAWAKPPDCHNDPAEARQVLMKLSREYQNV
                     ETQYPEIYKNYPVVELITMGVGGLIWERHGHIALCVRHSPTNEDCYNYGIGDFHHPVK
                     MGAGFFRGTHSFYVGKMDPGQMFMTYVCADRTLYVQPLPLTDAEEQEVINKLEFDIKE
                     ENKNYAYDHFWDNCTTRVRDVLDNATHGKLKTLKGPDDDSTYRDLARKGFFGMRIPLL
                     ITDIAMGRVTDRTPTYYEKMFLPDYLREAAEKAWGIKPVPVYTREGPPPLDDGPSGRL
                     IFALVILVLTSPAWIARLRRRFEKAGLAVAVLPYWFLGTVFWFLALISPLPYVRWNES
                     CLVLLPTDLLLVWFLSPERRQKYARFRVASIIVVAGLLLVNVLKQPLWPILLWPLVPA
                     AVVGFWPSKPKKA"
     gene            complement(3325..4374)
                     /locus_tag="VMZ53_25675"
     CDS             complement(3325..4374)
                     /locus_tag="VMZ53_25675"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS31927.1"
                     /translation="MEPLKTIGIDLWAGQARGVHDNRQTCFQTAPRIVAPKQMRALFL
                     GAYEKFAPLCREVDEPGLALLAVDEYTGAPAGLVRLRARPGRHVASIIGRHDRCDLFL
                     DKHSSLALRHLAVVLDPVQSWSRNSTSVHYRILDLRTERGFTDENGQAMRGLTAEGPA
                     FMRCAGHAIFVMPVGDPSDWPEKSSDAWDMLPERVYFDELRGDPRGSLTNMPAAPRNG
                     STGRSKSMIFRTLGPRDTGMGLVDRGDVAGSLDIIGPNLRGQISLGDRALRDGILIGR
                     YARCDGAALLDDPSMSRVHVLLLHVEGALLAIDTASRNGSHLPGQRKARVMQLDGDME
                     VHLGKQTRIRWRWAA"
     gene            complement(4414..5745)
                     /locus_tag="VMZ53_25680"
     CDS             complement(4414..5745)
                     /locus_tag="VMZ53_25680"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUS31928.1"
                     /translation="MDVAVTYLDSPGDVPARMPSPFAPEPVPIARRAMERLRAELPVE
                     TLRLLATDGKMFGVLVVRDGDRIGFLRAFSGMIGRSWHLPGFVPPAFDEAARDSFWIA
                     GEAELATLTDPSERAALSRTLLPRIHATYVLPNARGEVRALRDLFRAEVTGLQDKEPP
                     GGAGDCAAPKLLAYAYRHGLAPIALAEQWIGAPPTNDGRIDGNYYPACRGKCGPILAH
                     MLAGIDVEEAPQYGGGPISADEPRTLFEDDWLVIVDKPVGLLSIPGRGGALRDSVIMR
                     LRARYGSDVMVVHRLDLDTSGVLLAAKDAATYKALQRLFAIREVEKRYVAILDGQPSG
                     DRGTIDLPLRVDVDDRPRQIVDAVHGKPAITDWEVLAKAGGRTRVALSPRTGRAHQLR
                     VHMAVGLGAPIVGDRLYGRSDGGRLMLHAEALGFVHPHTGSRVAATREAPF"
     gene            5772..6080
                     /locus_tag="VMZ53_25685"
     CDS             5772..6080
                     /locus_tag="VMZ53_25685"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUS31929.1"
                     /translation="MNANVAKCLLVSKVLVADGMMSDDEKLFLQNMMEKLALTEAEKR
                     SVIELENWDEAEPIVTKLSEDERQELVEMLVDAASADGRLSAHELATVQRVSKALGLK
                     "
     gene            6115..6537
                     /locus_tag="VMZ53_25690"
     CDS             6115..6537
                     /locus_tag="VMZ53_25690"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HUS31930.1"
                     /translation="MASGFAQLKHEWQAFKDDEPGERFEDHHDRMKHASKGFNIGATA
                     LGVFLLLAGIVLCVIPGPGTPLIVFGLALLAARWQRAAKVLDRGELKVRKGGRRLKRW
                     WHALSKPAKAGVIIGLVILAAGASMFVWRFVVSAYLLG"
     gene            6537..7256
                     /locus_tag="VMZ53_25695"
     CDS             6537..7256
                     /locus_tag="VMZ53_25695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31931.1"
                     /translation="MQILLWLALATVSGQRARIDVDGEVSAKQAKQVAALVEAVVVDV
                     ERRFTQPAKQPDAHVTLLVYSDPDRYQTEAAKLGPVISDWGFYMPDKRTAIANVGASI
                     GNLRHELVHPLLGDDYPTIPAWLNEGIASLYGSAKLGKHGFTFLVNYRLRDLQKALKA
                     GTLPSLAELAASTADDLHGDQAMMFYAYSRYVLLYADKQGTLSKLYADLRAADHKKHA
                     AILASYVDETAFRAWARKLRY"
     gene            7353..>9003
                     /locus_tag="VMZ53_25700"
     CDS             7353..>9003
                     /locus_tag="VMZ53_25700"
                     /inference="COORDINATES: protein motif:HMM:NF023404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31932.1"
                     /translation="MDERNSKRDLVLAPGEYAYMQDVTKGVVKTYTGPTVINPTAQER
                     PIVFDETTQRFVPCSLDEAVQPVATAPEGSYLVLENPSARGDHPTAGGVHPAPDLAVG
                     RTLNLTGPRAFALWPGEVVKLVPGHHLRSNQYVLVRVLNEDEAKRNWAQAVMKPAEGG
                     ELTGPPAVLGVGQLLVIRGTDVSFYIPPTGLSVVPDAAGRYVRDAMTLERLEYCILVD
                     ENGNKRYERGPQVVFPEPTEVFVEQNGQRKFRAIELSELHGLHIKVIAPYSEGGRDYR
                     EGDELFITGKETAIYYPREEHSLIRVDGRERHVAVAIPAGEGRYVVDRTTGVIRVVHG
                     PAMFLANPIREVLVHRVLSDRQCATWYPGNASVLAHNKALRAGTETPAKPLPETRRAS
                     LVLDTRYEGSPHIVVWVGYAVMVVDPRGQRRVVTGPATILLEFDETLEILQLSTGTPK
                     TPDKRIETVYLRTLNNRISDRCTVETSDHVAITMSYALRVGFEGDPQRWFEVEDYVQL
                     LCDHVRSLLRAAIKRRSVDDVYANVVDVVRDAVLGAPTTDGS"
BASE COUNT         1565 a         2915 c         3001 g         1522 t
ORIGIN      
        1 acgacgtagc gcgccatctt cgcgcgagtc tcgaggaagt cggtgctcga tttctgcagc
       61 gccgcgtaga cctgccagag cgccgacgcc cagatctcgc cggcgttgtg cacttcggag
      121 ttgctgttca tgacgaggaa cggatggccc gacggtggcg cctcgccgct cgccatgtgg
      181 cggaacgaca gcgcgttgat cgagaagctc gcgctgtacg gcgcgcgtcg gatcccgtag
      241 tacgccggat cgttcgagta gccttgcgtg acgtagatgc cgaacgggta cgcgcggtcg
      301 aggttgtcgc ccgggcgaga cgtcagcatg agcgcggaga agtcgcccca cccttcgctg
      361 agtgcgcggc acatctggtt gccgcacagc gtgaggcggt gatggaggta gtggccgaac
      421 tcgtgtgcga tcagcgtcga gtcgagcgtg ccgtcgaggt ccgtgcccac ctcgcgatgc
      481 agcgtggccg tcacagtgcc cttcgcgatc gccgctttga gcgcagcgcc cgccacgtcg
      541 gtcaccgaga cggtgggaat cgtgatcgtc gtgttggcga gctgcgagtc gtcgccgagc
      601 ggtggcggtg tcgtgctcgt ctggttgttg acgatgagga cgccgacggc gtttgcgttc
      661 tgtgcgttga gcgccttgcg cttgaacgtg cagttgccgc gatcgacggc gacgagcttg
      721 ccgtcgatgt tgttcgtgta cggctcgcat ccgtcggtga cgttgacgcc cgcgccgtct
      781 tgaccgacga caaccgcgcc ttgcacgtcg aattgcttcg gcccgaacac cgcgacgccg
      841 aactcgggct tgtcccccga cgagaggtcg agcacgcggt tgtctttgcc cgaccacacg
      901 tacacctgca tgcgcggaga catgccgtcg tcgggggtgt tcatgttcgc gttgttgcgc
      961 gagccaccga gcgcgttgtc ttgcgcctcg gcgaggatcg catcgccttc gatgccaccg
     1021 cggccgtagt tcgacttctg cgcgttgccc gcagcctcgg tgaagccggc gtcgtaccag
     1081 aagtcgtgca gccagttgat gccgtagaac agcgacgtga tcgaggcgtt ctgctgttcg
     1141 gccgtgacga gcggcggcag ctccagatcg tagacgtacg agaacgcgcc ggctgacgag
     1201 acggtcgcgc ggaagtcgcc ttgcgtgagc ccgttcggcg ccgagacatc gatgtacgca
     1261 tcgacgttgt tgcccgaggt ctcggtggca cccggtggca gccacggatc gccagatgcg
     1321 ctgaggctgt cgacgctgac gaggttcggt cccgcgagat aggccggata gaatccgttc
     1381 ggcgagccgc tcgggtgcgg cgtggcgtcg acctgcggtc catcgagtgg gtgcttctcg
     1441 cccgtcggct cggcgaagac tttgtacgtg aacgcgtcgg cgctgaggct gtaacgctcg
     1501 agcacgcgac cgtcatcacc cgcgatgatc gtccgcatcg cgtcgccatg cgcgcttcca
     1561 ggatcgctcg tatacgcgtc gacaacccac gctgcgatca atgcatcgcc gctcggatac
     1621 cagacctcgc gagcgagagc ttgatcgacg tgtgcacctc gcgccgcccg cgcaatcgcc
     1681 atcgcgcgat catcgatgac gcgccacgac ctcgcgcgat cgcgaccgac gagcgtgccc
     1741 gtgatcgcgc gcagctcgct gtcttgcacg agcacgcgaa gctcgccgcc ccacacgggc
     1801 aagccatcga tcgtctgccg cacgcgcgtg atcgttccgc cgggaacgtt cagcgtaccg
     1861 acctcgtcga gcacgggcac cgcgagcggc gagacgcgcc acgccgacgc gaagtggagc
     1921 acgtgatcgc gagcagacga tgcgacaggc gcatcacgaa cgtcgagcat gcgaggcagg
     1981 ccgcgcgcgt cggcggatcg cgtgatcgtg ccggcgatcg gcttcttggg cggatcagcg
     2041 cacgcgacaa ctagcgctag cgcgagcgtc cacctcacgg ctcgacggta tcaggctttc
     2101 ttcggcttgg acggccagaa accgacaaca gccgctggca cgagcggcca caagagaatc
     2161 ggccacagcg gctgcttcag aacattcacg agcaagaggc ccgcgacgac gatgatcgac
     2221 gcgacgcgga accgcgcgta tttctggcgg cgctccggcg agaggaacca aacgagcagg
     2281 aggtccgtcg gcaggaggac gagacacgac tcgttccacc gtacgtacgg cagcgggctg
     2341 atcagcgcga ggaaccagaa cacggtgccg aggaaccagt acgggagcac ggcgacggca
     2401 agccctgcct tctcgaagcg gcgacgcagc cgcgcgatcc acgcaggtga cgtgagcacg
     2461 aggatgacga gcgcgaagat caggcgaccg ctcggtccat cgtcgagcgg cggcggtcct
     2521 tcacgcgtgt acaccggcac cggtttgatg ccccacgctt tctcggcggc ttcgcgcagg
     2581 tagtccggca agaacatctt ctcgtagtag gtcggcgtgc gatcggtgac gcggcccatc
     2641 gcgatatcag tgatgagcag tgggatgcgc atgccgaaga agcccttgcg tgcgaggtcg
     2701 cgatacgtgc tgtcgtcgtc ggggcctttg agcgtcttca gcttgccgtg cgtcgcatta
     2761 tcgaggacgt ctcgcacgcg cgtcgtgcag ttgtcccaga agtgatcgta cgcgtagttc
     2821 ttgttctctt ctttgatgtc gaactcgagc ttgttgatga cctcttgctc ttcggcgtcg
     2881 gtcagcggca gcggctgcac gtacagcgtg cgatcggcgc agacgtacgt catgaacatc
     2941 tgcccgggat ccatcttccc gacgtagaag ctgtgcgttc cgcggaagaa gccggcaccc
     3001 atcttcaccg ggtggtgaaa atcgccgatg ccgtagttgt agcaatcctc gttcgtcggg
     3061 ctgtgacgga cgcagagcgc gatgtgaccg tggcgttccc agatgagccc accgacgccc
     3121 atcgtgatga gctcgacgac cggatagttc ttgtagatct cggggtactg cgtctcgacg
     3181 ttttgatact cgcggctgag cttcatcagc acctgccgcg cttccgccgg atcgttatga
     3241 cagtccggtg gcttcgccca cgcaggcgcg gtcgtcacca ggatcaagag cgcgagcgca
     3301 aaccgcacgc cctgagtgta ttcgtcaggc ggcccagcgc cagcgaatgc gcgtctgttt
     3361 gcccaggtga acttccatgt cgccgtcgag ctgcatgacc cgcgccttgc gctgtccagg
     3421 caaatgactg ccattgcggc tggcggtgtc gatagcgagt aacgcgccct cgacgtggag
     3481 gagcaggacg tgaacgcgcg acatcgacgg atcgtcgagg agagccgcgc catcgcagcg
     3541 ggcgtagcgg ccgatcagga tgccgtcgcg aagcgcgcga tcgccgagcg agatctggcc
     3601 gcgcaggttc gggccgatga tgtcgagcga accggcgacg tcgccgcgat cgacgaggcc
     3661 catgccggtg tcgcgcggac cgagcgtgcg aaagatcatg ctcttgctgc ggcccgtcga
     3721 tccattgcga ggagccgccg gcatgttcgt cagtgatcct ctgggatcgc cgcgcagctc
     3781 gtcgaagtac acgcgctcgg gaagcatgtc ccacgcgtcg gaagatttct ccggccagtc
     3841 cgacggatca ccgacgggca tcacgaagat cgcgtgacct gcgcagcgca tgaacgccgg
     3901 gccttctgcc gtgagcccgc gcatcgcctg gccgttctcg tcggtgaagc cgcgctcggt
     3961 gcgcagatcg aggatgcggt agtggacgct cgtcgagttg cgcgaccagc tctgcacggg
     4021 atcgagcacg acggcgaggt ggcgcagcgc gagcgacgaa tgcttgtcga gaaacagatc
     4081 gcagcgatcg tggcgaccga tgatcgaagc gacgtgacgg cccggacgcg cgcgcagccg
     4141 aacgaggccc gctggggcgc cggtgtattc gtcgaccgcg aggagggcga gaccaggctc
     4201 gtcgacttcg cgacacagcg gagcgaactt ttcatacgcg ccgagaaaca gcgcgcgcat
     4261 ctgcttgggc gcgacgatgc gaggcgccgt ctgaaagcag gtctggcggt tgtcgtggac
     4321 gccgcgggcc tgcccagccc acagatcgat accgatcgtc tttagcggct ccactccctg
     4381 accctagcaa atacgtatga gcagcgacac gctttagaag ggtgcctcgc gggtagcggc
     4441 gacccggctg ccggtgtgtg ggtgtacgaa tccgagcgct tcggcgtgga gcatcaggcg
     4501 tcctccgtca gaccggccgt acagacgatc tccgacgatg ggagcgccca aaccgaccgc
     4561 catatggacg cgcagttggt gcgctcgacc ggtccgcggg gagagtgcga cgcgggtgcg
     4621 accgccggcc ttcgcgagga cctcccagtc ggtgatggct ggcttgccgt ggacggcatc
     4681 gacgatctga cgcggccggt cgtcgacgtc gacgcggagt ggcaggtcga tcgtgccgcg
     4741 atcgccgctc ggctgcccat cgagaatcgc gacgtaccgc ttctcgacct cgcggatcgc
     4801 gaagagccgc tgcagcgctt tgtacgtcgc cgcgtctttg gcggcgagca gcacgcccga
     4861 cgtatcgaga tcgaggcgat ggacgaccat cacgtcgctg ccgtagcgcg cgcgcagtcg
     4921 catgatgacc gaatcgcgaa gcgcaccgcc gcggcccggg atcgagagca agccaacggg
     4981 cttgtcgacg atgacgagcc agtcgtcctc gaacagcgtg cgtggctcgt ctgcggagat
     5041 cggtccaccg ccgtactgcg gcgcctcttc gacgtcgatg ccggcgagca tgtgcgcgag
     5101 gatcgggccg cacttgccgc gacacgccgg atagtagttg ccgtcgatcc ggccgtcgtt
     5161 cgtcggcggc gcaccgatcc attgctcggc gagcgcgatc ggcgcgaggc cgtggcgata
     5221 tgcgtacgcg agcagcttcg gcgccgcgca atctccagcg ccaccgggag gctctttgtc
     5281 ttgcagcccc gtgacttcgg cgcgaaacag gtcgcggagt gcacggacct cgccgcgcgc
     5341 attcggcaag acgtacgtcg cgtggatgcg tggcagcagc gttcgcgaga gtgctgcgcg
     5401 ctcgctcgga tcggtcagcg ttgcgagctc ggcctcgccg gcaatccaga acgaatcgcg
     5461 cgcggcctcg tcgaaggcag gcggcacgaa gccaggcagg tgccagctcc ggccgatcat
     5521 cccggagaac gcgcgcagaa atccgatgcg gtcgccatcg cgtacgacga gcacgccgaa
     5581 catttttccg tcggtggcaa gcaggcgcag ggtctcgacc ggcagctccg cacgcagccg
     5641 ctccatcgcg cggcgggcaa tcgggaccgg ctccggcgcg aacgggctcg gcatccgcgc
     5701 gggcacatcc ccgggcgaat ctaagtacgt cacggccacg tccatcgcgc gctcagcata
     5761 cagtcgcggc tatgaacgcg aacgtcgcga agtgcctgct cgtctccaaa gtgctcgtag
     5821 ctgacggcat gatgtcagac gacgagaagc tgtttttgca gaacatgatg gagaagctcg
     5881 ccctcaccga ggcagaaaag cgcagcgtca tcgagctcga aaactgggac gaggccgagc
     5941 cgatcgtcac gaagctttct gaagacgagc ggcaagagct tgtcgaaatg ctcgtcgacg
     6001 ctgcatctgc agatggccgc ctctcggcgc acgagctcgc aaccgtgcag cgtgtgtcaa
     6061 aggcgctcgg cctgaaatag tggccggcct gcgagtgcac ggctagcctt ttctatggcc
     6121 agcggttttg cgcagctcaa gcacgagtgg caggcgttca aggacgacga acccggcgag
     6181 cgtttcgaag atcaccacga tcggatgaaa cacgcttcga agggcttcaa catcggagcg
     6241 acagccctcg gcgtctttct cctcctcgcc ggaatcgtgc tctgcgtgat tccaggtccg
     6301 ggaacacctc tcatcgtatt cggcctcgcg cttctcgctg cgcgctggca acgcgctgcg
     6361 aaggtcctcg atcgtggcga gctcaaggtg cgaaagggtg gccgccgcct gaagcggtgg
     6421 tggcacgcgc tgtccaagcc ggcgaaagcg ggcgtcatca ttggcctcgt catcctcgca
     6481 gccggtgctt cgatgttcgt gtggcgtttc gtcgtctctg cgtacttact tgggtaatgc
     6541 agattctgtt gtggctggcg ctcgcaaccg tgagcggcca gcgcgcacgc atcgacgtcg
     6601 acggtgaagt cagcgcgaag caggcgaagc aagtcgcggc cctcgtcgaa gcggtggtcg
     6661 ttgacgtcga gcgacggttc acacagcctg cgaagcagcc cgacgcgcac gtcacgttgc
     6721 tcgtctattc ggatccagat cgctatcaaa cggaagccgc caagcttggc cccgtgatct
     6781 cggactgggg cttctacatg cccgacaagc ggaccgcgat cgcgaacgtc ggcgcgagca
     6841 tcggcaacct gcgccacgag ctcgtgcatc cgctgctcgg cgacgactat ccaacgatcc
     6901 cggcgtggct caacgaaggc atcgcctcgc tgtacggcag cgcgaagctc ggcaagcatg
     6961 gcttcacgtt cctcgtgaac taccgcctgc gcgatctcca aaaggcgctg aaggcgggga
     7021 cgctgccgtc gctcgcagag ctcgctgcgt cgacggccga tgacctccac ggcgaccaag
     7081 cgatgatgtt ctacgcgtac tcgcggtacg tgctgctgta cgccgacaag caaggcacgc
     7141 tgtcgaagct ctacgccgac cttcgcgctg ccgatcacaa gaagcacgca gccatcctcg
     7201 cgagctacgt cgacgagacg gcgtttcgcg cgtgggctcg caagctgcgc tactagttgc
     7261 ttcgccgtga agcagctgct ccgtcgtgga gcacgtcgcc ggatgcaact cgcgaggttg
     7321 gcgcggcatc gcacttgctg gaggtgccgc acatggatga gcgcaacagc aagcgggatt
     7381 tggtgctcgc gcccggcgag tacgcgtaca tgcaggacgt cacgaagggc gtcgtgaaga
     7441 cgtacacggg gccgacggtg atcaatccaa ccgcgcaaga gcggccgatc gtgttcgacg
     7501 agacgacgca gcgcttcgtg ccctgctcgc tcgacgaagc ggtgcagccc gtcgcgaccg
     7561 cgcccgaagg cagctatctc gtcctcgaga atccgtccgc gcgtggcgat cacccgaccg
     7621 cgggtggcgt gcatccggcg ccggatctcg cggtcggtcg cacgctgaac ttgacgggac
     7681 cgcgcgcgtt cgcgctgtgg cccggcgaag tcgtcaagct cgtgcccggc catcatctgc
     7741 gctcgaacca gtacgtgctc gtgcgcgtgc tcaacgagga cgaagcgaag cgcaattggg
     7801 cgcaggccgt gatgaagccg gctgaaggcg gcgagctcac aggtccgccc gccgtgctcg
     7861 gcgtcggcca gctgctcgtg atccgcggga ctgacgtctc cttctacatc ccgccgacgg
     7921 gcctctcggt cgtccccgac gccgccggtc gttacgtgcg cgatgcgatg acgctcgagc
     7981 gcctcgagta ctgcatcctc gtcgacgaga acggcaacaa acgctacgag cgcggcccgc
     8041 aggtcgtgtt ccccgagccg acggaagtat tcgtcgagca gaacggccag cggaagttcc
     8101 gcgcgatcga gctatcggag ctgcacggcc tgcacatcaa agtcatcgcg ccctacagcg
     8161 aaggtggccg cgactaccgt gaaggtgacg agctgttcat cacgggcaag gagaccgcga
     8221 tctactaccc gcgcgaggag cactcgctga tccgtgtcga cggtcgcgag cgtcacgtcg
     8281 ccgtcgccat tccggcgggc gaaggccgct acgtcgtcga tcgaacgaca ggcgtcatcc
     8341 gcgtcgtcca cggaccggcg atgttcctcg cgaatccgat ccgcgaggtg ctcgtgcatc
     8401 gcgtcctctc cgaccgccag tgcgcgacgt ggtacccggg caacgccagc gtgctcgcgc
     8461 acaacaaggc gctgcgcgca gggacggaaa cgccggcgaa gccgctgccc gaaacgcgac
     8521 gcgcatcgct cgtgctcgac acgcgctacg aaggctcgcc gcacatcgtc gtctgggtcg
     8581 gctacgccgt aatggtcgtc gacccccgag gccagcgccg cgtcgtcacg ggccccgcga
     8641 cgatcctgct cgagttcgac gagacgctcg agatcctcca gctctccacg ggcacgccaa
     8701 agacgcccga caagcgcatc gagaccgtct acctgcgcac gctgaacaat cgcatcagcg
     8761 atcgctgcac cgtcgagacg agtgaccacg tcgcgatcac gatgtcgtac gcgctgcgcg
     8821 tcggcttcga aggcgaccct caacgctggt tcgaagtcga agactacgtg cagctgcttt
     8881 gcgatcacgt gcgctcgctg ctgcgcgccg cgatcaagcg ccgctccgtc gacgatgtct
     8941 acgctaacgt cgtcgatgtc gtccgcgacg cagtgctcgg cgcgcctacg acggacggct
     9001 ccg
//
LOCUS       DATLBZ010000508        17343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5130670, whole genome shotgun sequence.
ACCESSION   DATLBZ010000508 DATLBZ010000000
VERSION     DATLBZ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17343
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5130670"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VMZ53_25705"
     CDS             complement(<1..250)
                     /locus_tag="VMZ53_25705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31933.1"
                     /translation="MGAAGATLIAAWLLTPRGPEAMPPDEMSSPVLSAKLSSKTLLAT
                     DRDQDIAVTITMPKGRAQVRPPLSLAIVIDRSGSMEGEA"
     gene            501..1157
                     /locus_tag="VMZ53_25710"
     CDS             501..1157
                     /locus_tag="VMZ53_25710"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS31934.1"
                     /translation="MTNAQERKAQERQARRRRIQEAARTVFAERGYAGASIELIARAA
                     QLSVGAIYLYFRSKEDLYVSLIEDALTVFDVEMGQVREHGDVSNRLKETWSILVRWAE
                     RDAEGPRILRLLAQPAIRPQLSDEVVTAVSAGVARIQDHIGACVADGIHAGLYRQVNA
                     REIADLAWSVLLGSLDAAEMHSNIGMPAEALVVRTDRALSLIESALAPQVAATLRAVA
                     "
     gene            1208..1651
                     /locus_tag="VMZ53_25715"
     CDS             1208..1651
                     /locus_tag="VMZ53_25715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31935.1"
                     /translation="MLRLVVVISLTLLAACGGGASYQISIRNASSRPIEELYVYPTGA
                     ARGTSRGQLAPNASTAVKMKGGHVSVTAISAKIQVDEHTRDKPTADQQVELNAPVELV
                     FYDDGAAPPGIERKGVIGVAFQIPKAAPKPDEGPPQPDEPAPQAP"
     gene            1758..3332
                     /locus_tag="VMZ53_25720"
     CDS             1758..3332
                     /locus_tag="VMZ53_25720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31936.1"
                     /translation="MRTDESGRFCPECGAALIEQAARQVEAAAPSGSSSSATKLIGLT
                     IDGQFEIEAIIGGGAFGTVYRGRQVGLDRPVAVKVPTFEIAADPVMAKRFAREARAAA
                     RVQHPGVVGIFAVGELADGRPYLAMQLVDGEPLDRALSDGPLPPARALRIARAIASAL
                     SETHAADVVHRDLKPTNVMWRRDRSGDDRITLVDFGIAVAKPGNAEATRLTANGLIGT
                     PHYMSPEQAHGEIVDARADLYALGCLLFELVTGTTPFEGSGFEVLLAHLGRPAPLPSS
                     KNPDVPECIDELCAALMQKKPDDRPANADTVVALIDEALEQLPDAMRVSQENPRAAAS
                     SSARKSRKPARTLATRHDLPEGTVTPSRRRRVTRHILSSLVAVAALGGAGFAVNQLRD
                     HPNAGIEPAADEGSAADNDPRIGRRRTITKDNGDVIATAAIPDPLLHGFESRARLDLK
                     NKLGQPLVAEEVVLTIADSSGAAKGLTARPRGAADKRVPGRYYFSYSFPQKGHYVVRV
                     FTPASDSTLEIPLDVE"
     gene            complement(3420..3953)
                     /locus_tag="VMZ53_25725"
     CDS             complement(3420..3953)
                     /locus_tag="VMZ53_25725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31937.1"
                     /translation="MSLRTIALSLVMAAFMASPALAGDPNAVFKGQIKLSTKRFPLSA
                     KSKDAYIAQVRKQSSTNFQENKETKSWKIYFAAFLKAPLNDVEYMVKFYEVGRGANQL
                     LGASEAFNDERGQRTIVSNITLDKKGFGVNKELMMTIESKGTVLASARFKILGEGEKF
                     TGKVNFSEDEAQGKEEE"
     gene            complement(3991..4881)
                     /locus_tag="VMZ53_25730"
     CDS             complement(3991..4881)
                     /locus_tag="VMZ53_25730"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HUS31938.1"
                     /translation="MSTARNPTTFIVGAGPVATALAGALRLAGVPVLGLWGRTPPAAR
                     AAGAAAGVAAFSSAPPDILLEAEVVVLAVRDSGITDAAQTLVGTGLVGKRHVLLHCAG
                     AAPAEEVLGPVREKVGGIGTMHPLQAIADGRAAMRALKGTVFGIEGDDRGKAIATKLV
                     TALGGVVLALDQTQMASYHCAAALASNYIVSALDAAAQVLAGAGVSPTQAVQALVPLA
                     EGAVKNVAAKGTTQGLTGPIRRGDVETIQRHLDAIAGKADLLEIYRALARRALEIAAR
                     LDGPDAPDRAGLEKIRQLIS"
     gene            complement(4878..5450)
                     /gene="nadD"
                     /locus_tag="VMZ53_25735"
     CDS             complement(4878..5450)
                     /gene="nadD"
                     /locus_tag="VMZ53_25735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HUS31939.1"
                     /translation="MRRIGLFGGSFNPPHAAHQLVALYALETLVDEIWVVPTYSHPFG
                     KQLVAYDHRIAMCELAVAALGARARVSRAEQELAHKPDFVASRTLDLIEHLYASFPDV
                     ELRLVVGADILHEKDKWYRWDDVVAKAPLIIIGRGGYELPEGSVATGITMPEISATRV
                     RELLAAGDPSVSSLLPQTVLRYIERHGLYR"
     gene            complement(5447..6622)
                     /locus_tag="VMZ53_25740"
     CDS             complement(5447..6622)
                     /locus_tag="VMZ53_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HUS31940.1"
                     /translation="MTVQTGLARLVDEGAKLIAGRRVGYLCNATSVDAELRHGIELLR
                     GAGVNITALFGPEHGVRGDAQDMIGVDAAVDTLTALPVHSLYGHTEASLSPTAEMLEG
                     IDVMVFDIQDVGSRYYTFVWTMVLAMRACAKAGKAFVVLDRPNPIGGVHVEGGAIDAG
                     FESFVGLVSCPNRHGMTAGEIARWRHSVEMMDLDLAVVSMRGWMRDLWFEHTGLPWVL
                     PSPNMPTIDTALVYPGMCLVEGTELSEARGTTKPFELSGAPHLDPHKLAAAMTAMDLP
                     GVICRPVIYTPTFHKHAMKPCGGVQLHVTNHDTFRPYRTGVAFLKACHDQAPAQFQWR
                     VKAYEFVDKIPAIDLLAGNAELRTGIEAGASIDDLARRWPRYEGEFAEERAAFLLYE"
     gene            complement(6619..8121)
                     /locus_tag="VMZ53_25745"
     CDS             complement(6619..8121)
                     /locus_tag="VMZ53_25745"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HUS31941.1"
                     /translation="MSRTVIAAAVAIVIAVLTAVAFFVTSKAYSDKVRKDAEVQLRRS
                     YQVVQQLAQLEAIDVANKAERLAADKSFATALKSESTSERANASRVGFTKFTSNEKEG
                     DIKPDLIALVDANGKLVAMSDVPSVVANQWIKPDKPNETIIPGLNVALGSRVIISDIW
                     NDRGRMMKIGIAPVIDTDVVAPPSDPDGVVIIGAIVVAYSQTAQLAQQDKRLLGTEIA
                     YYDQGKVVATSFTKGNGNEEDTAKTAQLTELIKSGKVKEGAGQVKVMIDGLPYYAAAV
                     PMPRSTTNSKRMPADYPAMTGGAMVLAAAQDSANTAGTIKMFIALLGIGAIAMSLLGL
                     YLTHRRLVTQVDQIELGVTDIINGNVDRTFRPVGAELDGLANGLNVMLARLLGRPEPG
                     EEEFDEEGNPIIPGRVDFEEGEDKPQVDPDLANLAKESEPDYYKRVFTEYVAARKAAG
                     SPDDVSFENFIAKLKVNEGKLRAQYNCRAVRFRVVTKDNKVSLKPVPIFE"
     gene            complement(8181..9878)
                     /locus_tag="VMZ53_25750"
     CDS             complement(8181..9878)
                     /locus_tag="VMZ53_25750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31942.1"
                     /translation="MNCPTCQVEIFDVDVKCANCGTVLGATGAHRMIGTLILGTYELV
                     DVLGQGGMSVVFKAKHKMTDQEVALKILPPELAAHSQVKSRFLEEAKALAALDHPNIV
                     HLYNFGQENGSFVLAMQFVRGKTWERAILENKRLDWKITCRIAIDVLKALEYAHGRGV
                     VHRDMKPSNVLVRESDGAATVMDFGIAKMTTSTKLTAEGQTMGTVRYMSPEQVRGQEV
                     DLRTDLYSLGATIYESLVGDTPFDGSTHFEIMTKHLSELPKAPSTHGVEVPRDVEDAI
                     MRSLAKKAGDRFESARDLRKILENALRTGDVGLVETQRINREILGDLKSTKDERPSFS
                     TPSTADSSAKALAPTARSATASDLADELEPGTTSAQARIGKPKSKLPWIILALLVVLG
                     GGATGAVMVLGAKRSPSYQARTKIKGVTLAQGKRVGTVFVETDGKVTPEEVEVTYEAV
                     LQDIKEFEPQIGGKRDVIEEIVAIPQTELCNLSIVRASRKPCNASDTATTALNVDKRQ
                     LLVSSDRATLADNLRFGVANAVCDFQFLLPEMASGTDEVVKKLDAICEMTKRFATPK"
     gene            complement(9875..10792)
                     /locus_tag="VMZ53_25755"
     CDS             complement(9875..10792)
                     /locus_tag="VMZ53_25755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS31943.1"
                     /translation="MAAGPAGITQRHITARDGTRIGYQVTGPAGAPCVVLANGLGGTY
                     IAFRYLYDALGEYRTICWDYRGLYTSALSSDPRANTVRHQVDDLIDICTAENVESFVL
                     AGWSMGVQVGFETLRHYPERVKGMLAINGTYGHAFRTVMGSRLVGRTIPMLLRLVKAQ
                     AALAGIATKRLAGSDALISAMKRVGIVSQTIDIEIFRAVAAGFQTIDWAIYSDLLTRL
                     DEHDAEEVLEHITVPVTIVTGDRDLMTPPSTAEHMHRLIKRSRLVVIEGGTHYTPVEY
                     PDIIVEELGTLLDRVPGWERRHHAKDAAS"
     gene            complement(10871..11758)
                     /locus_tag="VMZ53_25760"
     CDS             complement(10871..11758)
                     /locus_tag="VMZ53_25760"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUS31944.1"
                     /translation="MNRRLPVVLGALAFATLVGATSSNAEAGGRFSVRIGGQARVHVG
                     SSWHFARPAWRPNRWHVSGSIYVGPRYVYQPRPYYVYYPTYVPSYYEQTTYYPVAPAY
                     TTPGVTVAVAPRAPLPKLGIGLFAGGSAVEAQDGGGANESDDMGLLARFRITPGLVIE
                     GEMGKMSYDVDGYDNVRVDRRLGASLLYEIGAYNKWAPYILVGTGVQQAEVGGQFTTT
                     QDYGEIGAGLRWAVTPKFHLAFDLRAGSRSTVSSDSNNTTVPTTNTGVSARTITPPTE
                     TSDESEEYTRGRLSAILYF"
     gene            12040..13299
                     /locus_tag="VMZ53_25765"
     CDS             12040..13299
                     /locus_tag="VMZ53_25765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31945.1"
                     /translation="MSDKKAGNRDISDLKARLGLKKGGAAAPAAAPRANGAPSGGVLA
                     PPGMAVPPPPGAVSPAQPVIPNAADDPFAAMNAMAAVAQAKPQKEYIIVNDGKPVENV
                     GEKSNAASLLKILVPGLIALVIGVAVGKIGTSASDYNEGLRGAKAILGEKTTPSTVKS
                     LKSTLNEIDAVLDEAKTKNGLKPNLDLDKRLKELAAKLEVKLDVVFRAQQVNPVLSGQ
                     IMSFYAGVTEVKDMIDIHNKSAAFDDVMLKKGKESADKAASDVQGFEGETKYAVLVSA
                     PTDEDKNAEFGAKVVEIMGVYCGGNNMVSKCGEGEYPNAIGYRTDVGGPVIKGDLAQG
                     GSDNVPTKKVVKILSGTVKDGLIKGGEPAVSEVYYTKRLKALYDRIHGKPDQTGKPVG
                     GLIEDGNKLEQALSTEANKGTRFSFFM"
     gene            13335..14231
                     /locus_tag="VMZ53_25770"
     CDS             13335..14231
                     /locus_tag="VMZ53_25770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31946.1"
                     /translation="MGRIRSALLFSALLGLATSSALAADAKKKKAVPAKKVPAKKAEA
                     PKKAAPPPMVTADHKKALAEKMGGFKFGMTKDEILKVLQKQIDDRFEEKIKATTDVAT
                     QDKLRKEKKAELARTSSTYVKFDGKKTGWDVSIIETEFAHNTGESMMERWENEGGKNN
                     RRFFFFKDEKLWKMYISLDVSMIPEDKRNFDTFKGVMEGQYGKGAIDGGTISWRAGEF
                     DARAVDRLKDYGALGLVVEDPKVRNELVAAREAVAPKKAETPSVIKAVIDTEGKDHPD
                     VKSNSGSVEAVIQANGGGSTKK"
     gene            complement(14524..14844)
                     /locus_tag="VMZ53_25775"
     CDS             complement(14524..14844)
                     /locus_tag="VMZ53_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HUS31947.1"
                     /translation="MPTYEYRCNACKREFEAVQRMADPDLTHCTSCHEEKLEKLISFT
                     LVKSDVWKDALYQDKPKEAMKGTHAVDLAKPPKSTAQPSTLDDSFKPCNEACATSAAA
                     ERAR"
     gene            14863..15315
                     /locus_tag="VMZ53_25780"
     CDS             14863..15315
                     /locus_tag="VMZ53_25780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31948.1"
                     /translation="MRHAASTGCLVGLLCIAACHDCPEKPDDCVENDVTLTQGVYGQV
                     VEGDDVVHDGSCREYAQPVSYPVWFETTDGVRVAEDVADEHGAYELAMTSGEYRACVQ
                     DNNNNRRCGALRTVTVPTTGRVRYDIWTGVIGLTTAAVRGPSLCGREP"
     gene            complement(15323..>17343)
                     /locus_tag="VMZ53_25785"
     CDS             complement(15323..>17343)
                     /locus_tag="VMZ53_25785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31949.1"
                     /translation="FSAVALGKNYELLKNEFMALEFPDARRGRAVAKAILVDDYRIPN
                     QVGGIAHRGGSVLETPGSNGSDPALCPTWAGMTPPANVTPFCVIQEWVNRERASLGNQ
                     VTQMNQSDTVPVIFVERNTGDVRDRLEFDTFVGGADLMRATLTFGANFGGNPTVGGAT
                     SLLGNASCAGLAGGDIQAPDVAPDGDRVAFAARPSAGAPLSVWIVQLSTGQCTQVTPA
                     SGMIHNFDPAFSPDGRYLVFASTRGKSGPTQSRKRLLPQSDLWRIQLPTGTPEQMTFL
                     SNSEVSPQFMREGRVTMTTEKASDGFYQLAGRRLNWDLTDYHPLLAQRSASPYASLTD
                     LAQSGPSIGYSAATDIREGADGNFLLILSDVGTDGKSVVPGAGGALAVFNRSIGPFEQ
                     GRGDPGYLPSVKFVDSAATGRMGATTAYRAPTYLPDGQILVSYAPQATTANYRLVAMN
                     PRTGAQTTLLNPPGGKAIVDAVIAYAYPPRSLYLNRRQLVFGGTSTSDPTHSVLYMPD
                     APMIFTLLTGNLRRGRPVTSFRTAAKLVVETEGMCPAGSCTRGANGIFESRMTLGSAD
                     LADDGSVKVRLPAGVGVILSLVDSTGKTLVKMGEEHQLGPGEQVSMGVRETITNAAGQ
                     TVNLFDAICAGCHGSVSGRELDVSVSADALTGASASASQTADPTTIGN"
BASE COUNT         3001 a         5496 c         5814 g         3032 t
ORIGIN      
        1 gcgcctcgcc ttccatcgaa cccgaccgat cgatcacgat cgcgagtgag agcggcggac
       61 gaacctgcgc gcggcctttc ggcatggtga tcgtgactgc gatatcctga tcgcgatccg
      121 tcgcgagcag cgtcttcgac gagagcttcg cgctcaggac cgggctcgac atctcgtcgg
      181 gaggcatcgc ctcgggacct cgcggcgtca ggagccaggc ggcgatgaga gtggcgccgg
      241 cggcacccac gacgagcgaa gtcctggtcc gatgagcagt gagcatgcgg tctatcgacg
      301 catgaccgga gctgacgatc tggcgcgtat cagcaccggt gaacccggtt agaagcgaaa
      361 ttcgagtcag atttagccta catgccttgg aggctactcg agtagccacc gcatcactgc
      421 caactcgacg aatctactag ccattactag tgatgaagat gccgcttgac cgcttgcgtc
      481 aaagttcgta aagcaccgcc atgacgaacg ctcaagaacg caaggcgcaa gagcgacagg
      541 cccgccgccg tcgcatccag gaagcagccc gcacggtgtt cgcggagcgc ggatacgcag
      601 gtgcctcgat cgagctcatc gctcgcgcgg cccagctctc cgtcggcgcg atctacctct
      661 acttccgctc gaaggaagac ctctacgttt cgctcatcga agacgcactg acggtcttcg
      721 acgtcgagat gggtcaggtc cgcgagcacg gcgacgtgtc gaaccgtctc aaggagacgt
      781 ggtcgatcct cgttcgctgg gcagagcgcg acgcggaagg tccgcgcatc ctgcgcctcc
      841 tcgcgcagcc ggccattcgc ccgcagctgt cggacgaagt cgtgaccgcg gtgtccgcgg
      901 gcgtggcgcg catccaggat cacatcggcg cgtgtgttgc cgatggaatc catgcgggcc
      961 tctaccgcca ggtcaacgcg cgcgaaatcg ccgacctcgc ctggtcggtg ctcctcggca
     1021 gcctcgacgc tgcagagatg cactcgaaca tcggcatgcc ggccgaagcg ctcgtcgtcc
     1081 gcacggaccg cgccctctcg ttgatcgaga gtgcactcgc gccgcaagtc gccgcgactc
     1141 tccgcgctgt cgcctgattc tctgctcgct gttctacggc ccgtgtaaac gggtctagga
     1201 tagctgcatg cttcgtcttg tcgtcgtcat ctcgctcacg cttcttgcag catgcggtgg
     1261 cggagcgagc taccagattt cgatccgcaa cgcgtcgtcg cgaccgatcg aagagctgta
     1321 cgtctatccg accggcgctg cgcgcggaac atcgcgcggc cagctcgcac cgaatgcgag
     1381 cacggcggtg aagatgaaag gcggccacgt ttcggtgacc gcgatcagcg cgaagatcca
     1441 ggtcgacgag cacacgcgcg acaagccgac ggctgatcag caggtcgagc tgaacgcgcc
     1501 tgtcgagctc gtgttctacg acgacggcgc agcgcctccc ggcatcgagc gaaaaggcgt
     1561 catcggcgtc gcgttccaga tccccaaagc tgcgcccaag cccgacgagg gacctccgca
     1621 gccggacgag cccgcgccgc aagccccttg aatgtcgtcg gcgcagcgga agtgccgcgc
     1681 tgtgcagggt tttcgcatcg tgaagatccc agaggctgct acgttcaatc tctgtgcgga
     1741 tttgctcttc atgtgacgtg cgtaccgacg agtcggggcg gttctgcccc gagtgcggtg
     1801 ccgcgttgat cgagcaagcc gcgcgacagg tcgaggcggc cgcaccgtct ggcagctctt
     1861 cgagcgcgac gaagcttatc ggcctcacga tcgacggcca gttcgagatc gaagcgatca
     1921 tcggcggtgg cgcgttcggc acggtgtatc gcgggcgcca agtcggtctc gatcgccccg
     1981 tcgcggtgaa agtgccgacg ttcgagatcg cggcggatcc ggtgatggcg aagcggttcg
     2041 cgcgagaagc gcgtgcagcc gcgcgcgtgc agcatcccgg cgtcgtcggc atcttcgcgg
     2101 tcggcgagct cgccgatggc cgtccgtacc tcgcgatgca gctcgtcgat ggcgagccgc
     2161 tcgatcgcgc tctctccgat ggaccactgc cgccggcacg cgcgctgcgg attgcgcggg
     2221 cgatcgcgag tgcactctcg gagacgcacg ccgccgacgt cgtccatcgc gatctcaagc
     2281 cgacgaacgt catgtggcgt cgcgatcgca gcggcgatga tcgcatcacg ctggtcgact
     2341 tcggcatcgc cgtcgccaag cccggcaacg ccgaagcgac gcgcctcacg gcgaatggcc
     2401 tcatcggcac gccccactac atgtctcccg agcaagcgca cggcgagatc gtcgacgcgc
     2461 gcgcggatct ctacgcgctc ggttgcttgc tgttcgagct cgtcaccggc acgacaccgt
     2521 tcgaaggctc gggcttcgaa gtcctcctcg cgcacctcgg tcgccctgcc ccgctgccaa
     2581 gcagcaagaa cccagacgtg ccggagtgca tcgacgagct gtgcgcggcg ctgatgcaga
     2641 agaaaccaga cgatcggccg gcgaatgccg acaccgtcgt cgcgctgatc gacgaagcgc
     2701 tcgagcagct gcccgacgcg atgcgcgtct cgcaggaaaa cccgcgcgcg gcagcatcgt
     2761 cgtccgcacg caagtcgcgc aagcctgcac gcacgttggc aacgcgccac gatctcccgg
     2821 aaggcaccgt gacgccgtcg cgccgtcgcc gggtgactcg ccacatcctg agctcgctcg
     2881 ttgcggtcgc agcgctcggc ggtgcgggct tcgccgtgaa tcagctgcgc gaccatccca
     2941 atgccggcat cgagcccgcc gccgacgagg gcagcgcagc cgacaacgat ccgcgcatcg
     3001 gtcgccgccg cacgatcacg aaggacaacg gcgacgtgat cgcgaccgcg gcgatccccg
     3061 atccactgct gcacggcttc gagagccgcg cgcgcctcga tctgaagaac aagctcggcc
     3121 agccgctcgt cgccgaggaa gtggtcctga cgatcgcgga ctccagcggc gcagcgaagg
     3181 gcctcaccgc gcggccacgc ggcgccgccg acaagcgcgt gcccggccgc tactacttct
     3241 cgtactcgtt cccgcagaag ggccactacg tcgtgcgcgt gttcacgccc gcgagcgact
     3301 cgacgctcga gattccgctc gacgtggaat aggggtccgc ccccattgct ccgagatgga
     3361 gcagtccgga atgaaaaagc ccgcgcgagg cgggctgcga tgcggcgaag agcgagggcc
     3421 tactcttctt ctttgccctg cgcttcgtct tccgagaagt tcaccttgcc cgtgaacttt
     3481 tcgccttcgc cgaggatctt gaagcgcgcg ctcgcgagca cggtgccctt gctctcgatc
     3541 gtcatcatca gctctttgtt gacgccgaag cccttcttgt cgagcgtgat gttcgagacg
     3601 atcgtcctct gaccacgctc gtcgttgaac gcttcgctcg cgccgagcag ctggttcgcg
     3661 ccgcggccga cttcgtagaa cttcaccatg tactcgacgt cgttgagcgg cgccttgagg
     3721 aacgcggcga agtagatctt ccaggacttc gtttccttgt tctcttggaa gttggtcgac
     3781 gattgcttgc ggacctgcgc gatgtacgcg tctttgctct tcgcggagag cggaaaccgc
     3841 ttcgtcgaca gcttgatctg acccttgaag accgcattcg gatcacctgc gagcgcaggc
     3901 gacgccatga atgcagccat caccaacgag agtgcaatcg tacgaaggct catcgtccgt
     3961 cgatgataca caaattgcgg ggacgaattg tcagctgatc agctggcgaa tcttctcgag
     4021 gcccgcccta tcaggagcat ccggaccgtc gaggcgcgca gcgatctcga gcgcacggcg
     4081 cgcaagagcc cgataaattt cgagtaggtc ggccttaccc gcgatcgcat cgagatgacg
     4141 ttgaatggtt tcgacgtcgc cacgtcgaat ggggccggtc aggccctgcg tggttccctt
     4201 ggccgcgacg tttttgacgg cgccctcggc gagcggcacg agtgcttgca cggcctgtgt
     4261 aggagacaca cccgcaccgg ccagcacctg agcggccgca tctagcgcgc tgacgatgta
     4321 gttcgacgcg agagcggcag cgcagtgata cgaggccatc tgcgtttgat cgagcgcgag
     4381 cacgacgccg ccgagcgcgg tgacgagctt ggtcgcaatg gccttgccgc ggtcatcgcc
     4441 ttcgatgccg aacacggtgc ccttgagcgc gcgcatcgca gcacgaccat cggcgatcgc
     4501 ttgcagcgga tgcatcgtgc cgatgccacc gactttctcg cgcaccgggc cgagcacttc
     4561 ttcggcgggt gcggcgcccg cgcaatgcag cagcacgtga cgcttgccga cgaggccagt
     4621 accgacgagc gtctgcgccg catcggtgat gcccgagtca cgaaccgcga ggacgacgac
     4681 ctcggcttcg agcaggatgt ccggcggcgc ggacgaaaag gcggcgacac cagcagctgc
     4741 accagcggcg cgcgcggcgg gcggtgtacg tccccacagg ccgagcacag gtacgcccgc
     4801 gagacgcagc gcaccggcga gcgcggtcgc gaccggacct gcgccgacga tgaacgtggt
     4861 gggattgcgt gccgtgctca tcggtagaga ccgtgccgct cgatatacct caggaccgtc
     4921 tgaggcaaaa gcgacgagac actcgggtca ccggcggcca gcagctcgcg gacgcgcgtc
     4981 gccgaaatct cgggcatcgt gatgccggtc gcgacgctcc cttcgggcag ctcgtaccct
     5041 ccccgcccga taatgatgag cggcgccttc gcgacgacat cgtcccagcg gtaccacttg
     5101 tccttctcgt gcaggatgtc ggcaccgacg acgagccgca gctcgacatc gggaaagctc
     5161 gcgtacagat gctcgatcaa gtcgagcgtg cgactcgcga cgaagtccgg cttgtgcgcg
     5221 agctcctgct cggcccggct gacgcgcgca cgcgcaccga gagcggccac ggcgagctcg
     5281 cacatcgcga tgcggtgatc gtacgcgacg agctgcttgc cgaacggatg gctatacgtc
     5341 ggcacgaccc agatctcgtc aacgagcgtc tcgagcgcat agagcgcgac gagctggtgc
     5401 gccgcatgcg gcggattgaa gctgccgccg aacagtccga tgcgcctcat tcgtacaaga
     5461 ggaacgccgc cctctcctcc gcaaactcgc cttcgtagcg cggccagcga cgcgcaagat
     5521 catcgatgct cgcgcccgcc tcgatgccag tgcgcagctc cgcgttgccg gcgagcagat
     5581 cgatcgctgg aatcttgtcg acgaactcgt acgccttcac gcgccactga aactgcgcag
     5641 gcgcctgatc gtggcacgcc ttcaaaaacg cgacgccggt gcgatacggc cggaacgtgt
     5701 cgtgattcgt cacgtgcagc tgcacgccgc cgcatggctt catcgcgtgc ttgtgaaacg
     5761 tcggcgtgta aatgaccggc cggcagatca cgcccggcag atccatcgcc gtcatcgccg
     5821 cagcgagctt gtgcggatcc aagtgcggcg cgcccgagag ctcgaacggc ttcgtcgtcc
     5881 cgcgcgcctc cgagagctcc gttccctcga cgaggcacat gcccgggtac acgagcgcgg
     5941 tatcgatcgt cggcatgttc ggcgacggca agacccacgg caggcccgtg tgctcgaacc
     6001 acaggtcgcg catccagccg cgcatcgaga cgaccgccag atcgagatcc atcatctcga
     6061 cgctgtgccg ccaccgcgca atctcgcccg ccgtcatccc gtggcgattc ggacacgaca
     6121 cgaggccgac aaacgattcg aagcccgcat cgatcgcgcc gccctcgaca tgcacgccac
     6181 cgatcggatt cggccggtcg agcacgacga atgccttgcc cgccttcgcg cacgcgcgca
     6241 ttgcgagcac catcgtccac acgaaggtgt agtagcggct gccgacatct tggatgtcga
     6301 agaccatgac gtcgatgccc tcgagcatct ccgcggtcgg cgacagcgat gcctcggtgt
     6361 gcccatagag gctatgcacg ggcagcgccg tcaatgtatc gacggccgcg tcgacgccga
     6421 tcatgtcctg cgcgtcgccg cgcacgccgt gctcggggcc gaacagcgcg gtgatgttca
     6481 cgcccgcgcc gcgcaggagc tcgatgccgt ggcgcagctc tgcgtcgact gacgtcgcgt
     6541 tgcacaaata cccgacgcgg cgtcccgcga tcagcttcgc gccctcgtcg acgagtcgcg
     6601 cgagtcctgt ctgaaccgtc attcgaagat cgggacgggc ttgagcgaga ccttgttgtc
     6661 cttggtcacg acgcggaagc ggaccgcgcg gcagttgtac tgcgccctga gcttgccctc
     6721 gttgaccttg agcttcgcga tgaagttctc gaaggagacg tcatcgggcg agcccgcggc
     6781 cttgcgcgcg gcgacgtatt cggtgaacac gcgcttgtag taatcgggct cgctctcttt
     6841 ggcgaggttc gcgaggtcgg ggtcgacttg cggcttgtct tcgccctctt cgaagtcgac
     6901 gcgaccggga atgatcgggt tgccctcttc gtcgaactcc tcttcgccgg gctcggggcg
     6961 accgagcagg cgagcaagca tgacgttgag gccgttcgcg aggccgtcga gctcggcgcc
     7021 gacggggcgg aacgtgcggt cgacgttgcc gttgatgatg tcggtgacgc ccagctcgat
     7081 ctgatcgacc tgcgtgacga gccggcgatg cgtgaggtag aggccgagca gcgacatcgc
     7141 gatcgcgccg atgcccagca gcgcgatgaa catcttgatc gtgcctgcgg tgttggcgct
     7201 gtcttgcgcc gcggcgagca ccatcgcgcc acccgtcatt gccgggtagt cggcgggcat
     7261 gcgcttcgag ttcgtcgtcg agcgcggcat cggcacggcg gccgcgtagt acggcaggcc
     7321 gtcgatcatg accttcacct ggccagcgcc ctctttgacc ttgccgctct tgatgagctc
     7381 ggtcagctgc gcggtcttcg cggtgtcttc ctcgttgccg ttccccttcg taaagctggt
     7441 cgcgacgact ttgccctgat cgtagtaagc gatttcggtg ccgagcaggc gcttgtcctg
     7501 ctgggcgagc tgcgccgtct gcgagtacgc gacgacgatc gcgccgatga tgacgacacc
     7561 atctggatcg ctcggcggtg cgacgacgtc cgtgtcgatg acgggcgcga tgccgatctt
     7621 catcatgcgg ccgcggtcgt tccagatgtc gctgatgatc acgcgactgc cgagtgcgac
     7681 gttgagaccc ggaatgatcg tctcgttcgg cttgtcgggc ttgatccact gattggcgac
     7741 gacgctcggg acgtcactca tcgcgacgag cttgccgttc gcatcgacga gcgcgatcag
     7801 gtcgggcttg atgtcgccct ccttctcgtt cgacgtgaac ttcgtaaagc ccactcggga
     7861 cgcgttcgcg cgctccgagg tgctttcgct cttgagcgcc gttgcgaacg acttgtccgc
     7921 ggcgaggcgc tcggccttgt tcgcgacatc gatggcctcg agctgcgcga gctgctggac
     7981 gacctgatac gaccgcctga gctgcacctc cgcgtctttg cggaccttgt cgctataggc
     8041 tttcgacgta acgaaaaatg cgaccgctgt gagcaccgcg atcacgatcg cgacggcagc
     8101 ggcaatgacc gtgcgggaca tgctccctcc ggcgccgatt attcgtgcgg ttctgcgggg
     8161 atgtcaacga gatggcaggg tcacttcggg gtagcgaagc gcttcgtcat ctcacagatc
     8221 gcgtccagct tcttcacgac ctcgtcggtt cccgaggcca tctcgggcaa gaggaactgg
     8281 aagtcgcaga ccgcgttcgc gacgccaaat cgcaggttgt cggcgagcgt ggcccggtcg
     8341 ctcgacacga ggagctgccg cttatcgacg ttcagagcgg tcgtggcggt gtcgctcgcg
     8401 ttgcagggct tgcgtgaggc ccgcacgatc gacaggttgc agagctcggt ttgcgggatc
     8461 gcgacgatct cctcgatgac atcgcgcttg ccgccgatct gcggctcgaa ctccttgatg
     8521 tcctggagta cggcctcgta ggtcacctcc acctcttcgg gcgtgacctt gccgtcggtc
     8581 tcgacgaaca cggtgccgac gcgcttgccc tgcgcgagcg tgacaccttt gatctttgtc
     8641 cgcgcctggt agctcgggct gcgcttggcg ccgaggacca tcacggcccc ggtcgcgccg
     8701 ccgccgagca cgacgagcag cgcgagaatg atccacggca gcttgctctt gggcttgccg
     8761 atacgcgcct gtgcggaggt cgtgcccggc tcgagctcat cggcgagatc gctagcggtc
     8821 gcggagcgcg cggtaggcgc gagcgctttt gcgctgctgt cggcagtgga tggcgtagag
     8881 aagctcgggc gctcgtcctt cgtcgacttg agatcgccga ggatttcgcg gttgatccgc
     8941 tgcgtctcga cgaggccaac gtcacccgtg cgcagcgcgt tctcgaggat cttgcggagg
     9001 tcgcgtgcac tctcgaaccg atctccggcc ttctttgcga gcgagcgcat gatcgcgtcc
     9061 tcgacatcgc gcgggacctc gacgccgtgc gtgctcggcg ccttcggcag ctccgagaga
     9121 tgcttcgtca tgatctcgaa gtgcgtgctg ccatcgaacg gcgtatcgcc gacgagcgac
     9181 tcgtagatcg tcgcgccgag cgagtagagg tcggtgcgga gatcgacctc ctgaccgcgc
     9241 acctgctcgg gagacatgta gcggaccgtg cccatcgtct ggccttcggc ggtcagcttc
     9301 gtcgacgtcg tcatcttcgc gatgccgaag tccatgaccg tcgcggcgcc atcgctctcg
     9361 cgcacgagca cgttcgacgg cttcatgtcg cgatgcacga cgccgcggcc gtgcgcgtac
     9421 tcgagcgcct tcaggacgtc gatcgcgatg cggcacgtga tcttccagtc gaggcgtttg
     9481 ttctcgagga tcgcgcgctc ccacgtcttg ccgcgcacga actgcatcgc gaggacgaac
     9541 gagccgttct cctgaccgaa gttgtagagg tggacgatgt tcggatgatc gagcgccgcg
     9601 agcgccttcg cctcctcgag gaagcggctc ttcacctggc tgtgcgcggc gagctccggc
     9661 gggaggatct tgagcgcgac ctcctggtcc gtcatcttgt gcttcgcctt gaagacgacg
     9721 ctcatgccac cttggccgag gacatcgacg agctcgtagg tgccgaggat gagcgtaccg
     9781 atcatgcggt gcgcgccggt cgcgccgagc accgtgccgc agttggcgca cttgacatcg
     9841 acgtcgaaga tctccacctg gcacgtcggg caattcacga ggcggcatct ttcgcatgat
     9901 ggcggcgttc ccagccaggg acgcgatcga gcagcgtgcc gagctcctcg acgataatgt
     9961 cggggtattc gaccggcgtg tagtgcgtgc cgccctcgat cacgacgagg cgcgagcgct
    10021 tgatcaggcg atgcatgtgc tcggcggtcg atggcggcgt cattagatcg cgatcgccag
    10081 tgacgatcgt gacgggcacc gtgatgtgct cgagaacttc ttcagcgtcg tgctcgtcga
    10141 ggcgggtcag tagatcggag tagatcgccc agtcgatcgt ctgaaagccg gcggcgaccg
    10201 cgcggaagat ctcgatatcg atcgtctgcg agacgatgcc gacgcgtttc atcgcgctga
    10261 tgagcgcgtc gctgccggcg agccgcttcg tcgcgatgcc cgcgagcgcc gcctgcgcct
    10321 tcacgagccg gagcaacatc gggatcgtgc ggccgacgag ccggctgccc atcaccgtgc
    10381 gaaacgcgtg gccatacgtc ccgttgatcg cgagcatgcc cttcacgcgc tcgggataat
    10441 gacgcagcgt ctcgaagccg acctgcacgc ccatcgacca gccggcgagg acgaagctct
    10501 cgacgttctc cgcagtgcag atatcgatca gatcgtcgac ctggtggcgc accgtgttgg
    10561 cgcgcggatc gctcgagagt gcgctcgtgt agaggccgcg gtagtcccag cagatcgtcc
    10621 ggtactcgcc gagcgcgtca tagaggtagc gaaacgcgat gtacgtgccg ccgagcccgt
    10681 ttgcgagcac gacacacggc gctcccgcag gccccgtcac ctgatagccg atgcgggttc
    10741 cgtcgcgcgc cgtgatgtgg cgctgcgtga tcccagctgg cccggctgcc atgcacgagc
    10801 agcgtagccg aaaacgaaag cggccggggt ctacccagcc gccatcatta gtgagcgatc
    10861 gattagccga tcagaagtac aggatcgccg agagacgacc gcgcgtgtac tcctcgctct
    10921 cgtcgctcgt ctcggtcggc ggcgtgatcg tgcgcgccga aacacccgtg ttcgtggtcg
    10981 ggaccgtcgt gttgttcgag tcgctcgaca ccgtgctgcg gctgcccgcg cgaaggtcga
    11041 acgcgaggtg gaacttcgga gtcaccgccc agcgaaggcc ggcaccgatt tcgccgtagt
    11101 cctgcgtcgt cgtgaactgg ccgccgactt ccgcctgctg cacgcccgtg ccgacgagga
    11161 tgtatggcgc ccacttgttg tacgcaccga tttcgtagag cagcgacgcg ccgaggcggc
    11221 ggtcgacgcg gacgttgtcg tagccgtcga cgtcgtacga catcttgccc atctcgcctt
    11281 cgatgacgag gccaggcgtg atgcggaagc gagcgaggag gcccatgtca tccgactcgt
    11341 tcgcgccgcc accgtcttgc gcctcgacgg ccgagccgcc ggcgaagagc ccgatgccga
    11401 gcttcggaag cggagcgcga ggagccacgg cgacggtcac gccgggcgtg gtgtacgcgg
    11461 gagccaccgg gtagtacgtc gtctgctcgt aatacgacgg gacgtacgtc gggtagtaca
    11521 cgtagtaagg gcgcggctgg tagacgtagc gcgggccgac ataaatcgag ccgctgacgt
    11581 gccagcgatt cgggcgccac gcgggacgcg cgaagtgcca cgaggagccg acgtgaacgc
    11641 gggcctgccc gccgatgcga acggagaaac gacctcccgc ttcagcgttc gacgaggtgg
    11701 caccgacgag cgttgcgaac gcgagagcac cgaggacgac aggtaggcga cggttcactg
    11761 ggttcccttc tttctactca ctcggacgcg cgaaacgtcc agtcggttta ctgtgcaccc
    11821 gacgggccag cactttttcg acgacgacac gagtgaaacg agccaggttc cggcggctta
    11881 cattccgtcc agggatcgtc aggtgtgacc acgaattcac gacttaaggg actgtggtgc
    11941 tgcgaagaca tcttggatgc aacttgcgca actctccggc atccttgaca gttgcggcga
    12001 ctgattcata cagtcatgcc ccttcgggag gaacgacctg tgagcgataa gaaagccggc
    12061 aatcgagaca tcagcgacct caaggcacgc cttgggctca agaaaggtgg agcagctgcg
    12121 ccggccgccg ctcctcgcgc caacggtgca ccgtcgggcg gagtgcttgc gccaccggga
    12181 atggcagtgc cgccaccacc tggcgcagtg agtcccgccc aacctgtgat cccgaacgca
    12241 gcggatgatc cttttgcggc aatgaacgcg atggccgccg tcgcgcaggc caagccgcag
    12301 aaggaataca tcatcgtcaa cgacggcaag cccgtcgaaa acgttggtga gaaatcgaac
    12361 gcagcgtcgc tgctcaagat cctggtcccg ggtctcatcg ccctcgtcat cggtgtcgcc
    12421 gtcggcaaga tcggcacgag cgcgtcggac tacaacgaag gtctgcgcgg tgcgaaagca
    12481 attctcggcg agaagacgac gccgtcgacg gtgaagtcgc tcaagtcgac gctcaacgag
    12541 atcgacgccg ttctcgacga ggcgaagacg aagaacggcc tcaagccgaa cctcgatctc
    12601 gacaagcgtc tcaaggagct cgccgcgaag ctcgaggtca agctcgacgt cgtgttccgc
    12661 gcgcagcaag tcaacccggt cctctccggc cagatcatgt cgttctacgc cggcgtgacc
    12721 gaggtcaagg acatgatcga catccacaac aagtcggcgg cgttcgacga cgtcatgctc
    12781 aagaagggca aagagtcggc ggacaaagca gcgagcgacg tccaaggttt cgaaggcgag
    12841 accaagtacg cggtgctcgt ctcggcgccg acggacgaag acaagaacgc cgagttcggc
    12901 gcgaaggtcg tcgagatcat gggcgtgtac tgcggcggca acaacatggt ctccaagtgc
    12961 ggcgagggtg agtacccgaa cgccatcggc taccgcaccg acgtcggtgg tccggtcatc
    13021 aagggcgacc tcgcccaagg tggcagcgac aacgtgccga cgaagaaggt cgtcaagatc
    13081 ctcagtggca ccgtcaagga cggtctcatc aagggcggcg agccggcggt gtcggaggtc
    13141 tactacacca aacgcctaaa ggcgctgtac gaccgcatcc acggcaagcc cgatcagacg
    13201 ggtaagccgg ttggcggcct catcgaggac ggcaacaagc tcgagcaagc cctcagcacg
    13261 gaagccaata aaggcacgcg gttctcgttc ttcatgtgat gaatcttctg ggtctctgaa
    13321 acgtcggagg acgtatgggt cgcattcgct ctgcattgct cttctctgcc ctgcttggtc
    13381 tcgctacgag ctccgcgctc gccgccgacg ccaagaagaa gaaggccgtt ccggccaaga
    13441 aggtcccggc caagaaggcc gaggcgccga agaaagcggc cccaccgccg atggtcacgg
    13501 ccgatcacaa gaaagccctc gccgaaaaga tgggcggctt caagttcggc atgacgaagg
    13561 acgagatcct caaggtcctc cagaagcaga tcgacgatcg cttcgaagag aagatcaagg
    13621 cgacgacgga cgtcgcgacg caagacaagc tccgcaagga gaagaaggcc gagctcgcgc
    13681 gcacctcgtc cacctacgtc aagttcgacg gcaagaagac gggctgggac gtctcgatca
    13741 tcgagaccga gttcgctcac aacacgggcg agtcgatgat ggagcgctgg gagaacgagg
    13801 gcggcaagaa caaccgccgc ttcttcttct tcaaggacga gaagctctgg aagatgtaca
    13861 tctccctcga cgtctcgatg atccccgaag acaagcgcaa cttcgacacg ttcaaaggcg
    13921 tgatggaagg ccagtacggc aagggcgcga tcgacggtgg aacgatcagc tggcgcgcag
    13981 gcgagttcga tgcgcgcgcg gtcgatcgcc tgaaagacta cggcgctctc ggcctcgtcg
    14041 tcgaggatcc gaaagtccgc aacgagctcg tcgcggcgcg tgaagcggtc gcgccgaaga
    14101 aggccgaaac gccgtcggtg atcaaggcgg tcatcgacac cgaaggcaag gaccaccccg
    14161 acgtgaagtc gaactcgggt tcggtcgaag ccgtgatcca ggctaacggc ggcggctcga
    14221 cgaagaagta gtcacgtcgc agctcgctgc gcaccaacgc ctcggcccgc gccggggcgt
    14281 ttgtcgtttc ggcgcgccgc ggtccgcgcc cgggcatagt tcgtttcggc tctgcatcgg
    14341 gccgcgtgtc gttatggttg cgcgcgtcgg gccgggccgt ttgtcgtttc gggtcatgca
    14401 tggtcaggag cgtgcggcgg tggcggacgc tgcgcgcgac gtgcgtgcga gttgacgtga
    14461 cgttctccag gagctccatt accgcggcat gtgttcgggc cgcggtagac gcggcttacg
    14521 tggtcagcgc gcgcgttcag cagcggccga ggtcgcgcag gcttcgttgc agggcttgaa
    14581 cgagtcgtcg agcgtcgacg gttgagcggt gctcttcggc ggcttggcga ggtcgacggc
    14641 gtgcgtgccc ttcatcgcct ctttgggctt gtcttggtag agcgcgtcct tccagacgtc
    14701 gcttttcacg agcgtgaacg agatgagctt ctcgagcttc tcttcgtggc aggacgtgca
    14761 gtgcgtcagg tctggatctg ccattcgctg cacggcctcg aactcgcgct tgcaggcgtt
    14821 gcaccggtac tcgtaggtcg gcatcgagca taattagtcc gcatgcggca cgcggcgtca
    14881 acaggctgtc tggtcgggtt gctgtgcatc gcggcgtgcc acgactgtcc ggagaagccc
    14941 gatgactgcg tcgagaacga tgtgacactt acgcagggag tttatggaca ggtcgtcgag
    15001 ggtgatgacg tcgtccacga cggcagctgc agggagtacg cgcagccggt cagctatccg
    15061 gtgtggttcg agacgacgga tggcgtgcgg gtagccgagg acgttgctga tgagcacggc
    15121 gcttacgagc tcgcgatgac aagcggcgag tacagggcgt gcgtgcagga caacaacaac
    15181 aaccgccggt gcggggcctt gaggacggtg acggtgccga cgaccggacg tgtgcgttac
    15241 gacatctgga cgggcgtcat cgggcttacg accgcggcgg tgcgcgggcc atcgctctgc
    15301 gggcgcgagc cttagttcga gtttagtttc cgatcgtcgt cgggtcggcc gtttgcgagg
    15361 ccgaggcgga ggcgccggtg agtgcgtcgg cggagacgga gacgtcgagc tcgcgaccgg
    15421 agacggagcc gtggcagccg gcgcagatcg cgtcgaacag gttgacggtt tggccggcgg
    15481 cgttcgtgat cgtctcgcgc acgcccatcg agacttgctc gcctgggccg agctggtgct
    15541 cttcgcccat tttgacgagc gtcttgccgg tggaatcgac gagcgagagg atgacgccga
    15601 cgcctgcggg gaggcggact ttgaccgagc cgtcgtcggc gaggtcggcg gagccgagcg
    15661 tcatgcgcga ttcgaagatg ccgtttgcgc cgcgcgtgca ggagccggct gggcacatgc
    15721 cttcggtttc gacgacgagc tttgcggcgg tgcggaagga agtgacgggg cggccgcgcc
    15781 gcaggttgcc ggtgagcagc gtgaagatca tcggcgcgtc gggcatgtag aggacggagt
    15841 gggtcgggtc ggaggtggat gtgccgccga acacgagctg gcggcggttg aggtagagcg
    15901 accgcggcgg gtacgcgtac gcgatgaccg cgtcgacgat cgctttgccg cctggcgggt
    15961 tgagcagcgt cgtttgcgcg cccgtccgcg ggttcatcgc gacgaggcgg tagttcgcgg
    16021 tggtggcttg cggcgcgtag ctgacgagga tttggccgtc ggggaggtat gtgggggcgc
    16081 ggtatgcggt ggtggcgccc atgcggccgg tggctgcgct gtcgacgaat ttgacggacg
    16141 gcaggtagcc cggatcgcca cgaccttgtt cgaacgggcc gatcgagcgg ttgaagaccg
    16201 cgagcgcgcc gcccgcgccg gggacgacgg attttccgtc ggtgccgacg tcactgagga
    16261 tgaggaggaa gttgccgtcg gctccttcgc ggatgtcggt ggcggcggag tagccgatcg
    16321 aggggccgga ctgcgcgagg tccgtgagcg acgcgtatgg agaggcgctg cgctgcgcga
    16381 gtagcgggtg gtagtcggtg aggtcccagt tgaggcggcg acctgcgagc tggtagaagc
    16441 cgtcgctcgc tttctcggtc gtcatcgtca cgcggccttc gcgcatgaac tgcggcgaga
    16501 cttccgagtt ggagaggaac gtcatttgtt ctggcgtgcc ggtgggcagc tggatgcgcc
    16561 agagatcgga ttgcgggagc aggcgtttgc ggctttgcgt cgggccggat ttgccgcgcg
    16621 tcgacgcgaa cacgaggtag cggccgtccg gagagaacgc gggatcgaag ttgtggatca
    16681 tgccgctcgc cggcgtgact tgtgtgcatt ggcccgtcga gagctgaacg atccagacgc
    16741 tgagcggcgc gcctgcggat gggcgtgcgg cgaacgcgac gcgatcgcca tcgggcgcga
    16801 cgtcgggcgc ttgaatgtcg ccgcctgcga gaccggcgca ggacgcattg ccgaggagcg
    16861 aggtggcgcc accgacggtc gggttgccgc cgaagttcgc gccgaacgtg agcgttgcgc
    16921 gcatgagatc ggcgccgccg acgaacgtgt cgaactcgag gcgatcgcgg acgtcaccgg
    16981 tgttgcgctc gacgaagatg accgggaccg tgtcgctctg gttcatctgc gtgacttgat
    17041 tgccgagcga cgcgcgctcg cgattgaccc attcttggat cacgcagaac ggcgtgacgt
    17101 tggcaggtgg cgtcatgccg gcccacgtcg ggcaaagcgc cgggtccgag ccgttcgagc
    17161 ccggcgtctc gagcacggag ccgccgcgat gtgcgatgcc gccgacttgg ttcgggatgc
    17221 ggtaatcgtc gacgaggatc gccttggcga cggcgcgacc acgacgtgcg tcggggaact
    17281 cgagcgccat gaactcgttc ttgagcagct cgtagttctt gccgagcgcg acggcggaga
    17341 aga
//
LOCUS       DATLBZ010000509         2625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_663894, whole genome shotgun sequence.
ACCESSION   DATLBZ010000509 DATLBZ010000000
VERSION     DATLBZ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663894"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VMZ53_25790"
     CDS             <1..219
                     /locus_tag="VMZ53_25790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31950.1"
                     /translation="LELRRLAFPHAYSKGSTMAVWLWNSRNEYAISHAISCWLRDEAT
                     TVGDAEAIELTTRNCKLSVPTRTGDVRG"
     gene            complement(216..812)
                     /locus_tag="VMZ53_25795"
     CDS             complement(216..812)
                     /locus_tag="VMZ53_25795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31951.1"
                     /translation="MRGLPLVVLVLAGCAVHTVSTSLRKDQVGSKYARISGYMATRGA
                     APGGTHTEETPGSIRHDSILDEAVLAKVDAQETCVELVLRTDSGHDEPVDQYQPSFEI
                     DGKKTRGVIENELVSVYDYTFTGMRETASVEGVAASQYIGMSLQQPTDQIFRVIERRA
                     TVCGGIGGNVSQVKLDVTHPSWDVADYHYHLDFDWKLN"
     gene            complement(902..2053)
                     /locus_tag="VMZ53_25800"
     CDS             complement(902..2053)
                     /locus_tag="VMZ53_25800"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HUS31952.1"
                     /translation="MKRIVIALVVLIAASVLAGERETAWSPVVYPEQRIPLTFSHQKH
                     LGRGTQCDACHPSASTSRSAVDNLIPTEAQCRTCHAIERDKPDKQATPVAACKACHPG
                     FSPGVPVERVYLTPTPLKFDHSAHAKQTCESCHAVRQIDFATTKQLPTMSSCFACHEQ
                     ETDACTTCHLAAIGGLMQTTFPHGRLEPRSATLGDDHGPDFLRDHKQQARRLDSTCSA
                     CHDRSQCVECHNGVVKPVEFHAANYVLLHSVEAKRGKPDCSACHRQQTFCTGCHERSG
                     VGTRSDTELNSVDAGKQFHPAGWASKTTGVGNMHAIVAKRSIGSCASCHREDDCLECH
                     SAEASALKVSPHPAGWRASSRCRALDRSNRRMCLRCHVTDDELGCDWSK"
     gene            complement(2050..>2625)
                     /locus_tag="VMZ53_25805"
     CDS             complement(2050..>2625)
                     /locus_tag="VMZ53_25805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31953.1"
                     /translation="VRLRRGDHALEPALEYFFPTFDGDSIFNAFSLEPTTDVRVGYQY
                     TPTGPLRAHASGWLRAYDDAPGLSRFAGGADAGVEHAFGGGWRARVDGLWDDGWGGRR
                     IGGAADAAVRGRGRMWWRGRVVVLGVAEDETANVATARRFVTTSTVLSATYRIAETVA
                     VHAIGEADYDVIHDLQLRALAVVDLNFSPEP"
BASE COUNT          424 a          858 c          886 g          457 t
ORIGIN      
        1 ctcgagctgc gtcgcctcgc gttcccgcat gcgtatagca agggttcgac gatggctgtg
       61 tggctgtgga actcgcggaa cgagtacgcc atctcgcacg cgatctcgtg ttggctgcgc
      121 gacgaagcga cgaccgtcgg cgacgccgag gcgatcgagc tcacgacgcg caactgcaag
      181 ctcagcgtac cgacgcgaac cggcgacgtt cgcggctagt tcagcttcca gtcgaagtcg
      241 aggtgatagt ggtagtcggc cacatcccac gacggatgcg tgacgtcgag cttcacctgg
      301 ctgacgttgc ctccgatgcc gccgcatacg gtcgcgcggc gctcgatcac gcggaagatc
      361 tgatcggtcg gctgctgcag cgacatgccg atgtactggc tcgcagcgac gccttcgacg
      421 gacgccgtct cgcgcatgcc cgtgaacgtg tagtcgtaga cagagacgag ctcgttctcg
      481 atcacgccgc gcgtcttctt gccgtcgatc tcgaagctcg gctgatactg atcaaccggc
      541 tcgtcgtgac cgctgtcggt gcgaaggacg agctctacgc acgtctcttg cgcgtcgacc
      601 ttcgcgagca ccgcctcgtc gaggatggag tcgtggcgaa tcgagcccgg cgtctcttcg
      661 gtgtgcgtgc cacccggcgc tgcgccgcgc gtcgccatgt agccgctgat ccgtgcgtac
      721 ttcgagccaa cctgatcctt gcgaagcgag gtgctgacgg tatggacggc acagccggcc
      781 agaacgagta caacgagtgg gagtccgcgc atggggcgat gtcgtgtctc ggagcgccgc
      841 gccagtcaag gacaaagcag cgcgcctaca aacttcgtta caaagccggc gcgttcgcgc
      901 actactttga ccagtcgcag cccagctcgt cgtcggtgac gtggcagcgc aggcacatgc
      961 ggcggttgct gcgatcgagc gcgcgacacc gcgagctagc tcgccaaccg gcgggatgcg
     1021 gcgagacctt gagcgcgctc gcttccgcac tgtgacactc gaggcaatcg tcctcgcggt
     1081 gacacgacgc gcacgagccg atgctgcgct tcgcgacgat cgcgtgcatg ttgccgacgc
     1141 ccgttgtctt gctcgcccag cccgctggat ggaactgctt gcccgcgtcg acgctattga
     1201 gctcggtgtc gctacgcgtg ccgacgccgg atcgctcgtg gcaaccggtg cagaacgtct
     1261 gctggcgatg gcacgccgag caatcgggct tgccgcgctt ggcctcgacc gagtgcagca
     1321 gcacgtagtt tgccgcgtgg aactcgacgg gctttacgac cccgttgtgg cactcgacgc
     1381 attgcgagcg gtcgtggcac gcgctgcatg tcgaatcgag ccggcgcgct tgctgtttgt
     1441 gatcgcgcag gaagtcgggc ccgtgatcgt cgccgagcgt cgcgctgcgc ggctcgagcc
     1501 ggccgtgcgg gaacgtcgtc tgcatgaggc cgccgatcgc cgcgaggtgg catgtcgtgc
     1561 acgcgtcggt ctcctgctcg tgacacgcaa agcagctact catcgtcggc agctgcttcg
     1621 tcgtcgcgaa atcgatttgg cgaacggcgt ggcagctctc gcacgtttgc ttggcatgcg
     1681 cgctgtgatc gaacttgagc ggcgtcggcg tgagatacac gcgctcgacg ggcacgcctg
     1741 gggagaagcc cggatggcag gccttgcacg cggccaccgg cgtcgcttgc ttgtccggct
     1801 tatcgcgctc gatcgcgtga cacgtgcggc actgcgcctc ggtcgggatc aagttgtcga
     1861 ccgcggagcg agacgtcgac gcgctcggat gacaggcatc gcattgcgtg ccgcggccca
     1921 gatgcttctg atgcgagaac gtcagcggga tgcgttgctc tggatagacg accggcgacc
     1981 acgcggtctc tcgctcgccg gcgagcacgc tcgcagcgat gaggacgact agcgcgatga
     2041 cgatgcgttt catggctcgg gtgagaagtt gaggtcgacg acggcgagcg ctcgcagctg
     2101 cagatcgtga atgacgtcgt agtcggcttc gccgatcgcg tgaactgcga ccgtctcggc
     2161 gatccgatac gtggcgctca gtaccgtcga cgtcgtcacg aagcggcgcg cggtcgcgac
     2221 attcgcggtc tcgtcttcgg cgacgccgag cacgacgacg cgacctcgcc accacatgcg
     2281 tccacgtccg cgcacggcag cgtccgccgc gccgccgatc cgtcggcctc cccagccgtc
     2341 gtcccagagg ccatcgacgc gagcgcgcca gccgccaccg aacgcatgct cgacgcctgc
     2401 gtccgcacca ccggcgaagc gagacaagcc gggcgcgtcg tcataggcgc ggagccaacc
     2461 gctcgcgtgc gcacgcagcg gccccgtcgg cgtgtactga tagccgacgc gaacgtccgt
     2521 ggtgggctcg agcgagaacg cgttgaagat cgagtccccg tcgaacgtcg ggaagaaata
     2581 ttcgagcgcc ggctcgagtg cgtggtcgcc gcgtcgaagg cgcac
//
LOCUS       DATLBZ010000510         6278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2896851, whole genome shotgun sequence.
ACCESSION   DATLBZ010000510 DATLBZ010000000
VERSION     DATLBZ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2896851"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VMZ53_25810"
     CDS             <1..1081
                     /locus_tag="VMZ53_25810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31954.1"
                     /translation="RVDVYALGCIMYEMLVGKTPLKGDSMIRTIAMQMLDPIVPPSQV
                     NPDLHVPPALEAIVMKALEKKREQRFSTMGDLLTALEQIAPRLADAPSPSLPPLPPGA
                     DPALAPSTPTSLITNTGPRPLSSSESAVMTKVRRPKPATRQLHEPEFAAPGKPVSFTH
                     LDSEPEPRPQSSRVPMILAGLLMLLLGAGGMILLLMKLQGSNDDKPVAETPRDAALVV
                     TEQRDAAVIETPADAQVIVDVPVDAGVVAQAKRDAGVGVVRVNGGAIDKTKPVTIEVL
                     TRPGEADVFIGANYRGPSGVKIQERFGAKVHIECKTARMKGSLDVTFDGKVTAVMCTA
                     TRDRFCVPGIKNPFDDCEEDPNAGP"
     gene            complement(1153..2322)
                     /locus_tag="VMZ53_25815"
     CDS             complement(1153..2322)
                     /locus_tag="VMZ53_25815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31955.1"
                     /translation="MRNAGRSLLFAAVCSAGAGLLTSCSSAYADRIAAPSAPGPRIAL
                     AVAGEKLRAPYDVQVVTEDGDTAPTYAFRDRFYVQGSANQRYTIRVTNPTGRRVEAVV
                     SVDGLDVVDGESGDLNKRGYIVPAYGEVRIEGFRTSLQDVASFRFTSVDNSYAGRKGK
                     ARNVGVIAVAIFEETAPPPDVEIVAPVNPRPTYRWEDDVDDRDWGSDEDAEASVSRNR
                     GGSRHVPSRKDAAKADKRTAAAEESRKEVAPGDYYAPAPTTTATGRGGYAGGAAQGAP
                     AARPYMPPPPPPHQDVDDGVEAEWQPPVERAPNRTNRPGLGTEFGESRYSAASYTKFV
                     RAAGRPIAIAELRYNDVAGLMALGIPVAPMPDDGEIMMRETADPFPGDHFARAPR"
     gene            2405..3076
                     /locus_tag="VMZ53_25820"
     CDS             2405..3076
                     /locus_tag="VMZ53_25820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31956.1"
                     /translation="MAASKSVAKGAKAGGAKAKAKASAKPAAKPNGNGKSPKRRAAAD
                     VEFADPALFDPLTPGETADALRTLIEDRRVSSMAKVGRYRIICTEPLVVKPPHWMAGH
                     RLARVVAYDYAADRAVDACVDLDAGIVAHLELNRGQPMLSRDEEAIAISIAMADDRVR
                     SKLEMNDTAQSVMHYWGRSPNELAYARRSAAVIFGRAAGHSSLIAVVDLIDQQVTQVT
                     PAELW"
     gene            3076..3885
                     /locus_tag="VMZ53_25825"
     CDS             3076..3885
                     /locus_tag="VMZ53_25825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31957.1"
                     /translation="MSNTNVIEEANWKFHWRLTESAGIMIYLADYKGKRVLWEGSLPY
                     VTVDHQRETFDVEMAAEAHGQWWTPLGTRTIQGPVRVQSFRGGVELSASFASGPYQYT
                     QLWRFHDDGRLCPWLTIYGPGVHDQHTYHPHFRFDFDLDGAEEDAFERFEDGRWQRVA
                     QEGWFPYSGEADEAGNVWRQVDFRSRAAINLRPHSWDDAEVFAIRYHDGEWAPFSPRS
                     AAGSQPFPAAYVGAEELDGQDLSLWYVAHVHFDQSFPYTAGPWIKVEGLAK"
     gene            complement(3888..4742)
                     /locus_tag="VMZ53_25830"
     CDS             complement(3888..4742)
                     /locus_tag="VMZ53_25830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31958.1"
                     /translation="MTLADACVELAWAQWVAIGVSGSARPPHHAVDLEAAIAFAPALD
                     DLDPRLHDEVVDWCSRFAERVVSVSRLKQVLKLFDEDHQQRFERFAAIINARGGTKWP
                     TTSRAAPTKMTSEKSRFLIDGAAAVQLRARMIFGIGARADVLVSLLLSPLGWTHVSLL
                     ASLAYTKRSLADALGDLSAGGVLHGYLVGNAMHYRLVRKAELSALVGKHPELAFQPWA
                     QRLAVTASLLATTARTKTKSEMIRDIELRKTIDRHRVALASVGEEPVTSTNAEELVSA
                     WLLPLLEA"
     gene            complement(4745..5311)
                     /locus_tag="VMZ53_25835"
     CDS             complement(4745..5311)
                     /locus_tag="VMZ53_25835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31959.1"
                     /translation="MLTAKQLEDALSALGEVLADRGKRFEVAIIGGGALAIANLIARP
                     TKDLDVVAMINDGRLETAAPLPADLVEAIADVAAYLGLASDWLNNGPTAMLRFGLPEG
                     FLARCDRRAYGGLVVRFADRLDHIHFKLYAAADDRPGGKHHRDLVALAPTRDELRAAA
                     RWAREHDPSREFSQLVDQVLASFEEDGA"
     gene            complement(5402..6025)
                     /locus_tag="VMZ53_25840"
     CDS             complement(5402..6025)
                     /locus_tag="VMZ53_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HUS31960.1"
                     /translation="MSQHQVSADDVEKTIVSFTRLDQRGDKKAMNKLAQRLQKEQPFL
                     LQHAAAVRSEHGDAVGEAAVFYATLVWAMFDRIGDGTLPRLTAQNLAEADRVVTEELS
                     KVEGLADKPPHERVAPALVATQPHIYAKLSELLMEDVKEAAMTADTASTIIKPTEVVV
                     EAFDAAISGRRPGERQGTIVATNKVGRNEPCPCGSGKKYKKCHGEAQ"
BASE COUNT         1140 a         2092 c         1978 g         1068 t
ORIGIN      
        1 ccgcgtcgac gtctacgcgc tcggctgcat catgtacgag atgctcgtcg gcaagacgcc
       61 gctcaagggc gactcgatga tccggacgat cgcgatgcag atgctcgatc cgatcgttcc
      121 gccgtcgcag gtcaatcccg acctccacgt cccgcccgcg ctcgaagcga tcgtgatgaa
      181 ggcgctcgag aagaaacgcg agcagcgctt ctcgacgatg ggcgacctac tcactgcgct
      241 cgagcaaatc gcgcctcgcc tcgccgatgc tccatcaccg tcgttgcctc cgttgccgcc
      301 tggcgccgat cccgcacttg cgccatcgac gccgacctca ctgatcacga acacggggcc
      361 gcgcccactc tcgtcgtcgg aatcagcggt gatgacaaag gtgcggcgcc ccaagccggc
      421 gacgcgccag ctccacgagc ccgagttcgc cgcgccgggc aagccggtct cgttcacgca
      481 tctcgattca gagccggagc cgcggcccca gagctcgcgc gtgccgatga tcctcgcggg
      541 cctgctcatg ctgctgctcg gcgcgggcgg catgatcttg ctgctcatga agctgcaggg
      601 cagcaatgac gacaagccgg tcgcggagac gccgcgcgat gccgccctcg tcgtcacgga
      661 gcagcgcgat gccgcggtga tcgagacgcc cgccgacgcg caggtgatcg tcgacgtgcc
      721 ggtcgatgcg ggcgtcgtcg cgcaggcgaa gcgagatgcg ggtgtcggcg tcgtgcgcgt
      781 gaacggcggc gcgatcgaca agacgaagcc cgtcacgatc gaagtgctca cgcgtccggg
      841 cgaagcggac gtcttcatcg gtgcgaacta tcgcggcccg agcggcgtaa agatccaaga
      901 gcgcttcggc gcgaaggtcc acatcgagtg caagaccgct cggatgaagg gcagcctcga
      961 cgttacgttc gacggcaagg tcaccgcggt gatgtgcacg gcgacgcgcg atcgcttctg
     1021 cgtgcccggt atcaagaatc cgttcgacga ctgcgaagaa gatccgaacg ctgggccgtg
     1081 attacggccc gaaacgggca aagccgccac gagggcggct gggcggctag gcgagagcga
     1141 tcgcgatgcg attcagcgag gagcgcgcgc gaagtgatcg ccggggaacg ggtccgcggt
     1201 ctcgcgcatc atgatctcgc cgtcatcggg catcggcgcg acggggatgc cgagcgccat
     1261 caggccggca acgtcgttgt agcggagctc ggcgatcgcg atcgggcggc cggcagcgcg
     1321 gacgaacttc gtgtagctcg cggccgagta gcgcgactcg ccgaactcgg tgccgaggcc
     1381 cgggcgattc gtgcgattcg gcgcgcgctc gacgggcggc tgccactcgg cctcgacgcc
     1441 gtcatcgacg tcctgatgcg gaggcggcgg aggcggcatg tacggacgcg ccgccggtgc
     1501 accttgcgct gcgccaccgg cgtagccacc gcggcccgtt gccgtcgtcg tcggcgcggg
     1561 tgcgtagtag tcacccggcg cgacttcctt gcgcgattcc tcggccgcgg ccgtgcgctt
     1621 gtcggccttc gccgcgtctt tccgcgacgg gacgtggcgc gagccaccgc gattgcgcga
     1681 caccgaagct tcggcatctt cgtcgctgcc ccagtcgcga tcatcgacgt cgtcttccca
     1741 gcgatacgtc gggcgcggat tcacgggcgc gacgatctcg acatcgggcg gcggcgcagt
     1801 ctcttcgaag atcgcgacgg cgatcacgcc gacgttgcgc gctttgcctt tgcggcccgc
     1861 gtacgagttg tcgaccgacg tgaagcggaa cgacgcgacg tcttgcaacg acgtgcggaa
     1921 accttcgatg cgaacttcgc cgtatgcggg aacgatgtag ccgcgcttgt tgagatcgcc
     1981 gctctcgcca tcaacgacgt cgagaccatc gacgctgacg accgcttcga cacggcggcc
     2041 cgtcgggttc gtcacgcgaa tcgtgtagcg ctggttcgcc gagccctgca cgtagaagcg
     2101 atcgcggaac gcgtacgtcg gcgcggtgtc gccgtcttcg gtgacgacct gaacgtcgta
     2161 gggcgcgcga agcttttcac cggcaaccgc cagcgcgatg cgcggaccgg gcgccgatgg
     2221 agcggcaatc cgatcggcat acgccgacga gcacgacgtc aaaagcccgg caccagccga
     2281 gcaaaccgca gcaaagagaa gcgagcgtcc agcgttcctc atggtctttc aaacgcccga
     2341 gcccgccgcg agatctaaac aaaagcgctt gtcgtttcgt ggatttacat agatagtgcg
     2401 cgacatggct gcgtcaaaga gcgttgctaa aggggcgaaa gcagggggcg ccaaggcgaa
     2461 agccaaggcc agcgccaagc cggccgccaa gccgaatggc aacggcaaat cacccaagcg
     2521 cagggccgcg gcggacgtcg agtttgccga tcccgcgctc ttcgatccat tgacgcccgg
     2581 cgagaccgcc gacgccctcc ggacgctgat cgaggatcgc cgcgtgtcgt cgatggcgaa
     2641 ggtcggccgc taccggatca tctgcaccga gccgctcgtc gtgaagccgc cgcactggat
     2701 ggcaggtcac cggctcgcgc gcgtggtcgc gtatgattac gccgccgatc gcgcggtcga
     2761 cgcatgcgtc gatctggacg cgggcatcgt cgcgcatctc gagctgaaca gaggccagcc
     2821 gatgttgtcg cgcgatgaag aagcgatcgc gatctcgatt gcgatggccg acgaccgcgt
     2881 ccgcagcaag ctcgagatga acgacaccgc gcagtctgtc atgcactact ggggtcgctc
     2941 gccgaacgag cttgcctacg cgcgtcgcag cgccgccgtg atcttcggtc gcgccgcagg
     3001 ccattcatct ctcattgcag tcgtcgacct catcgatcag caggtcactc aagtcacccc
     3061 agcggagctc tggtgatgag caacacgaac gtcatcgaag aagccaactg gaagtttcat
     3121 tggcgcctga ccgagtcggc cgggatcatg atctatctcg ccgactacaa gggtaagcgc
     3181 gtcctctggg agggctcgct accgtacgtc accgtcgatc atcagcgcga gacgttcgat
     3241 gtcgagatgg ccgccgaggc gcatggtcaa tggtggacgc cactcggcac gcgcacgatc
     3301 caaggcccgg ttcgcgtgca atcgttccgc ggcggcgtcg agctctccgc gagcttcgcg
     3361 tcaggcccgt accaatacac gcagctctgg cgctttcacg acgacggccg cctctgccca
     3421 tggctcacga tctacgggcc gggcgttcac gaccagcaca cgtaccaccc gcacttccgc
     3481 ttcgacttcg atctcgacgg cgccgaggaa gacgcattcg agcgcttcga agatggccgc
     3541 tggcagcgcg tcgcccaaga aggctggttc ccgtactccg gcgaagccga cgaagcaggc
     3601 aacgtgtggc gccaagtcga cttccgatcg cgcgccgcga tcaacctgcg cccgcacagc
     3661 tgggacgacg cagaggtctt cgcgattcgc tatcacgacg gcgagtgggc accgttctca
     3721 ccgcgcagcg ctgccggctc gcagccgttc cccgctgcct acgtcggcgc cgaagagctc
     3781 gacggccagg acctctcgct ctggtacgtc gcgcacgtgc acttcgacca atccttcccg
     3841 tacaccgcgg gaccgtggat caaggtcgaa ggtctagcga agtagcgcta cgcctcgaga
     3901 agcggcaaga gccacgcgga cacgagctct tcggcgttcg tcgacgtgac aggttcttcg
     3961 ccgacgctcg caagtgcaac gcgatgtcga tcgatcgtct tacgaagctc gatgtcgcga
     4021 atcatttccg acttggtctt cgtccgcgca gtcgtggcga gcaatgacgc tgtcaccgct
     4081 aggcgctgcg cccacggctg aaacgcgagc tctggatgct ttcccacgag tgcgctgagc
     4141 tccgccttgc ggacgagtcg gtagtgcatg gcgttaccca cgagatatcc atgcagcacg
     4201 ccgccagcgc tcaagtcgcc cagtgcatcg gcgaggctgc gcttcgtata ggcgagtgat
     4261 gcaagcagac tgacgtgcgt ccagccgagc ggtgacagca acagcgacac cagaacatcc
     4321 gcgcgcgcac cgatcccgaa gatcatgcga gcccgcagct gcacagcggc ggcgccgtcg
     4381 atcaggaagc gcgatttctc gcttgtcatc ttcgtcggcg cggccctcga ggtggttggc
     4441 cacttcgtcc caccacgcgc attgatgatt gccgcgaaac gctcgaaccg ctgctggtgg
     4501 tcttcgtcga acagcttgag cacttgcttg agccgcgata ccgacaccac gcgttccgcg
     4561 aagcgcgagc accaatcgac cacttcgtca tgcaagcgcg gatcgagatc gtccaaagcg
     4621 ggcgcaaacg caattgctgc ttctagatcg actgcatgat gcggcggacg tgccgaaccc
     4681 gacacgccaa tcgcgaccca ctgcgcccac gcgagctcca cgcatgcatc cgcaagcgtc
     4741 acggctacgc tccgtcctct tcaaacgagg ccaggacttg atcgactaac tgcgaaaact
     4801 cgcgtgaggg atcgtgttcc ctcgcccacc ttgccgcggc ccgcagctca tcgcgagtcg
     4861 gcgcgagcgc tacaagatcg cgatggtgct taccgccggg acgatcatct gcggctgcat
     4921 atagcttgaa atgaatgtga tcgagacgat ccgcaaaccg cacgacaagc ccgccgtacg
     4981 ctctgcgatc gcagcgcgca aggaagccct ccggtagtcc gaaccggagc atcgccgtcg
     5041 gaccgttatt gagccagtca gatgcaagac ccaaatacgc agcaacgtcc gcgatcgctt
     5101 cgacgagatc agcaggaagc ggcgcggcgg tctcgaggcg gccatcgttg atcatcgcga
     5161 cgacgtcgag atctttcgtc ggacgtgcga tgaggtttgc gatcgcaagc gcaccgccgc
     5221 cgatgatcgc gacctcgaac cgcttgcctc ggtcggcgag gacttctccc agtgcggaga
     5281 gcgcgtcttc cagctgcttc gcggtcagca cacaagcact ttaagctccc ggaccgggaa
     5341 gataaagtgc ataaaacgcg aggcttatcc tcgcgtaaat ctttaatttc gggcagttac
     5401 gttactgggc ctcgccgtgg cacttcttgt acttcttccc gctgccgcac ggacacggct
     5461 cgttgcgacc gaccttgttc gtcgcgacga tcgttccctg tcgttcgccc ggacgacgtc
     5521 ccgaaatcgc cgcatcgaat gcttcgacga cgacttcggt cggcttgatg atcgtcgacg
     5581 ccgtgtccgc ggtcatcgcg gcttccttca catcttccat cagcagctcg gacagcttcg
     5641 cgtagatgtg cggctgcgtc gccaccaacg ccggagccac gcgctcgtgc ggcggcttgt
     5701 ccgcgagccc ttcgaccttc gagagctcct cggtcacgac gcgatccgcc tcggcaagat
     5761 tctgcgcagt aaggcgcggc agcgtgccat cgccaatgcg atcgaacatc gcccacacga
     5821 gcgtcgcgta gaacaccgcc gcctcgccga cggcatcgcc gtgctcgctg cgaaccgccg
     5881 ctgcatgctg cagcaagaac ggctgctcct tctggagccg ttgcgcgagc ttgttcatcg
     5941 ccttcttgtc gccgcgctga tcgaggcgcg tgaacgaaac gatggtcttc tcgacgtcgt
     6001 ctgcggagac ctggtgctgg gacatcggcg agatcttgcc gcgatgccgc ggcccgtcaa
     6061 gcgcttggcg ccagcgcatt cggaaaagtt caggcttctt cggctaacag caccttcact
     6121 tctcgaagga gccgctcgat caatccgagg tcaaaaatgc ggccggaggc gctcgtgaaa
     6181 cgccacttct agcgacccgt tctggtcctt ggtgacatcg aggatgcacg cgtcgccgtc
     6241 gacaagctcg tagatcggat agtcgcgatc gataggcg
//
LOCUS       DATLBZ010000511         4332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5869703, whole genome shotgun sequence.
ACCESSION   DATLBZ010000511 DATLBZ010000000
VERSION     DATLBZ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5869703"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VMZ53_25845"
     CDS             complement(<1..890)
                     /locus_tag="VMZ53_25845"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS31961.1"
                     /translation="MSAHVLVGRQRELGELATALADAEAGRGALVLIRGEPGIGKSRL
                     ASAFVESAVGARVAWGRAWEAGGAPAYWPWIEAFRALWQQAPADERDGLPKSRLAELV
                     QMDGELAAHTGDLGTPPQLEPQQARFRLFDAASAVIAHFAARSPLVIVLDDLHAADPA
                     SVEMLQFVARTVRTTRAVIVATYRDSEARSRAEVADAIARLSRDGRTIALSRLAETAV
                     SEWVAQEGRPELAHAVYARSEGNPLFVVELLRLAEELGDRGLSEIPDSVRDVIRQRLG
                     TLPPVTREVLQTAAVLGRVV"
     gene            complement(905..1966)
                     /locus_tag="VMZ53_25850"
     CDS             complement(905..1966)
                     /locus_tag="VMZ53_25850"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS31962.1"
                     /translation="MTIDDDKLNALLGKFVSDFGAALHATTVIIGEKLGLYRALADAG
                     KPLAARELAEHTGTTERYVAEWLAAQAASGYVEYDAATGRYSMTREQALVLADDTSPT
                     YVPGAFLIAASVFKDEPKITAAFRTGHGVGWHEHHADLFHGTARFFRPGYAANLVGSW
                     LPALDGVVAKLEAGARVADIGCGHGITTVLMAKAFPRSTFIGFDYHAPSIEAAREEAR
                     RAGVADRVKFEVASAKDYPGTGYDLVAFFDCLHDMGDPVGAAAHVKQSLAPNGTWMLV
                     EPFAHDRVEQNLNPVGRVYYSASTCICTPASCAQEVGLALGAQAGEGRLREVLLAGGF
                     RSVRRATETPFNLVLEAKL"
     gene            complement(1963..2523)
                     /locus_tag="VMZ53_25855"
                     /pseudo
     CDS             complement(1963..2523)
                     /locus_tag="VMZ53_25855"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            2653..>4332
                     /locus_tag="VMZ53_25860"
     CDS             2653..>4332
                     /locus_tag="VMZ53_25860"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS31963.1"
                     /translation="MLVGRDREIAVVDGALAQLAEGRGAVALVSGEPGIGKTRLVSEA
                     ATRAATRGARVAWGRCWEAGGAPAFWPWYEALGGLGLRFPDAATIAASDPAQARFALF
                     RDVAAELARAAVRSPLVLVLEDLHAADQSSVLLLEFLATQLRAAPIMVLGTYRDLEAS
                     LRAEVGDVIARSGRAGTVLALGRLRQQDVAAVVREAVEDADEALIARVFETTSGNPLF
                     VTELVQQVRTGASSSTIPLGVREIIRQRLGLVSPAARLMLDAGAVLGVEFGSGDIARM
                     ATNADPEIDAAIASGLVTRRGDRVRFTHALYREALYHDLPRATRHALHRDAARVLSAM
                     GAPAAEVAHHLIEAGPEAAPDAVEQTIVAARQALDAFAFEDAVSLLERAKLAVPAGPL
                     EATLRARIVIALGEVRLRSGDPKGRELCVEGAQRARELGDASLLALAGLAYGSVFLIG
                     GVDPVMVSMLEDALAGLPAVDSGLRARTMARLAAARQPSAPALRPRDIELALEAVGIG
                     RRVATRRELLEILQSASGALYGAADPRVRITISRDQAQLAEELGDAPRLIAA"
BASE COUNT          705 a         1474 c         1407 g          746 t
ORIGIN      
        1 ttgacgacgc gcccgaggac ggcggctgtc tgcagcacct cacgcgtcac cggtggcagc
       61 gtaccgagac gctggcggat cacatcgcgc acgctgtccg gaatctccga gagcccgcga
      121 tcgccgagct cctcggcaag ccgcaacaac tcgacgacga acaacggatt gccttcgctg
      181 cgcgcataca ctgcatgcgc gagctcgggg cgcccttctt gcgctaccca ttcgctcacc
      241 gcggtctccg cgagcctcga cagcgcgatc gtgcgtccat ctcggctcaa gcgcgcgatt
      301 gcatcagcta cctctgcccg cgaccgcgcc tcgctatcgc gatacgtcgc gacgatgacc
      361 gcgcgagtcg tgcgcactgt ccgcgcgacg aactggagca tctccacact cgccgggtcc
      421 gctgcatgaa gatcgtcgag cacgatcacg agcggcgacc gcgcagcaaa atgcgcgatc
      481 acggccgagg ccgcgtcgaa caaacgaaac cgcgcttgct gcggctcgag ctgcggcggc
      541 gttccgagat cgcccgtgtg cgcagcgagc tccccgtcca tctgcacgag ctcggcgagc
      601 cgcgacttcg gcaacccgtc gcgctcgtcg gcgggcgctt gctgccacag cgcgcgaaac
      661 gcttcgatcc acggccaata tgccggcgcg ccacctgctt cccatgctcg tccccacgca
      721 acgcgcgcac cgacggcgct ctcgacgaac gcgctcgcga gccgtgactt gccgatgccc
      781 ggttcgccgc ggatcaggac caacgctccg cgtcccgctt cggcgtcggc gagcgcggtc
      841 gcaagctcac ccagctcccg ctgtctccct accagcacgt gtgccgacac cgaggcgagt
      901 ttactcagag ctttgcttcg agtacgagat tgaacggcgt ctctgtcgcg cgacggacgg
      961 agcggaaccc tccggccagc agcacttcgc gcagcctgcc ctcgcctgcc tgcgcaccga
     1021 gcgcgagccc gacttcctgc gcgcacgacg ccggcgtgca gatgcacgtc gaagcgctgt
     1081 agtaaacgcg accgacggga ttgaggtttt gctcgacgcg atcatgcgca aacggctcga
     1141 cgagcatcca cgtgccattc ggcgcgagcg actgcttgac gtgcgcagca gctccgactg
     1201 ggtcacccat gtcgtgcaag caatcgaaga acgctacgag gtcgtagccc gtgccgggat
     1261 aatccttcgc agaagcgact tcgaacttca cgcgatcggc gacgcccgcg cgccgcgcct
     1321 cttcacgtgc cgcttcgatc gaaggcgcgt gatagtcgaa gccgatgaac gtcgagcgcg
     1381 ggaacgcctt cgccatcagc accgtcgtga tgccgtgccc gcagccaatg tccgcaaccc
     1441 gtgcgcctgc ctcgagcttt gcgacgacac cgtcgagcgc tggcagccag ctcccgacaa
     1501 ggtttgccgc gtaccccggt cgaaaaaacc gcgccgtgcc gtgaaacaga tcggcatggt
     1561 gctcgtgcca accaacgccg tggccggtgc gaaatgcggc cgtgatcttc ggctcgtctt
     1621 tgaacaccga cgccgcaatc agaaacgcgc ccggcacgta ggtcgggctc gtgtcatcgg
     1681 caagcaccag cgcttgctcg cgcgtcatcg aatatcggcc ggtcgcggcg tcgtactcga
     1741 cgtagcccga tgccgcttgc gccgcgagcc actcggccac ataacgctcc gtcgtgccgg
     1801 tgtgctcggc gagctcccgc gccgcgagcg gcttgccggc atcggcgagc gccctataga
     1861 ggccgagctt ctcgccgata atcacggtcg tcgcgtggag cgcggcgccg aagtcagaca
     1921 cgaactttcc tagaagcgca ttgagcttgt cgtcatcgat ggtcatgccc gcagggcgtg
     1981 actttcctcg tcacggttgt gactttcgac gattttggcg aggtgcttgc tcgctgcctc
     2041 cgacaggccg agccgcggta agtcttcacg aagctgcgcg cgcgtccccc gaccggcgac
     2101 gtagccgttt aggcgcgctg caacggtgga ctcgacaaac tgcgccggcg tgtatcccgc
     2161 gagctctcgc atcaggatcg agtcgtgtcg caagtagtac ttctggtgat agtcctccgc
     2221 cagatagaac ggcgcggccg taatgattgg cgccgtaatg ccgcgctcgc gagatatgcg
     2281 ctcctgctcg gcgttcgctg cgaacaccgc ctccatgtac tgacggctgt acgcgcgacc
     2341 gccgcggcga ttcttccaga tcgcgtcgag cagttgcgcg taactcagaa gctgcggatc
     2401 gaagtcgagc tggaacacct cggtgtgatc ggcgaggtcg tgataggtgg gatcgacggt
     2461 gcggccaccc gcgtagccga cgcgcgtgcg gacgatcgct ggatgacacc cgaaccgggc
     2521 atcaggcgtc cagaatcaac caagtccaaa cgttgcggtc tcgacgacac gcggctgctg
     2581 atcgagtcga agcatcatgc cgagtgtacg cggcagagtc gcacttggtt actactgatc
     2641 taagatggct cgatgctggt cggtcgggat cgggagattg cggtggtgga cggcgcgctc
     2701 gcgcaactcg ccgaggggcg cggcgcggtc gcgctggtct ccggtgagcc aggcatcggc
     2761 aagactcgac tcgtcagcga ggcggccact cgcgctgcga cacggggagc gcgcgtcgca
     2821 tggggacgct gctgggaagc cggtggcgcg ccggcgttct ggccatggta cgaggctctc
     2881 ggtgggctcg gcctgcgctt tccggacgcc gccacgatcg cggcgagtga tcccgcgcaa
     2941 gcgcggttcg ccttgtttcg cgacgtagcc gcggagctag cgcgcgcggc ggtgagatcg
     3001 ccgctcgtgc tcgtcctcga agatcttcat gctgcagatc aatcgagcgt gctcttgctc
     3061 gagtttctcg cgacccagct gcgagcggca ccgatcatgg tcctcggcac ttatcgcgat
     3121 ctcgaagcga gcttgcgcgc ggaggtcggc gacgtgatcg cgcggagcgg gcgcgcaggg
     3181 acggtgctcg cgctcgggcg cttgcggcaa caagatgtgg ccgctgtcgt gcgcgaagct
     3241 gtcgaggacg ccgatgaagc gttgatcgca cgcgtcttcg agacgacgag tggcaatcca
     3301 ctgttcgtta ccgagctcgt ccagcaagtc cgaacaggcg cgtcatcgtc gacgattcca
     3361 ctcggggtgc gagagatcat tcgccaacgc ctcggccttg tctcacccgc cgcgcgtctg
     3421 atgctcgacg caggcgcggt cctcggtgtc gagtttggca gcggcgacat cgcgcgaatg
     3481 gccacgaatg cggatccgga gatcgacgct gcgatcgcga gcggtctggt gacgcgacgc
     3541 ggcgatcgcg tgcgattcac acacgcactc tatcgcgagg cgctctatca cgatctgccg
     3601 cgtgcgactc gccacgcgct ccatcgcgac gccgctcgcg tgctgagtgc catgggtgct
     3661 ccggccgcag aggttgcgca tcaccttatc gaagccggtc ccgaggcagc gccggatgcc
     3721 gtcgagcaaa cgatcgtcgc tgcgcgtcag gcgctcgatg cattcgcgtt cgaagatgcc
     3781 gtctcgctgc tcgagcgcgc gaagctggcc gttccagcag gacctctcga agcaacactt
     3841 cgcgctcgaa tcgtgattgc gctcggcgag gtgcgccttc gcagcggcga cccaaaaggc
     3901 cgcgagctct gcgtcgaggg tgcgcagcgt gcgcgcgagc tcggtgatgc atcgctcctc
     3961 gcgctcgcag gcctcgcgta cggctcggtg ttcctcatcg gtggcgtcga tcccgttatg
     4021 gtctcgatgc tcgaagatgc gttggctggc ctgcctgcgg tcgacagcgg cctgcgtgct
     4081 cgcacgatgg ctaggttagc agccgctcgt cagccatccg ccccggcgtt gcggccgcgc
     4141 gacatcgagc tcgcgctgga ggccgtcggg atcggacgtc gcgtcgcgac ccgtcgagag
     4201 ctgctggaga tcctgcaatc cgcatccggt gcgctctacg gcgctgccga tccgcgcgtc
     4261 cgtatcacga tctcgcgcga tcaagcgcag ctcgcagaag agctcggtga cgcgccccgc
     4321 ctcatcgccg cc
//
LOCUS       DATLBZ010000512        12011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2898234, whole genome shotgun sequence.
ACCESSION   DATLBZ010000512 DATLBZ010000000
VERSION     DATLBZ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12011
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2898234"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="VMZ53_25865"
     CDS             <1..1673
                     /locus_tag="VMZ53_25865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS31964.1"
                     /translation="GVAEPKSYLRVEPERVDALMSSAGELLFDRTRIERRVQLLRTLA
                     RDLARTRQTLSDAIAREAPGKASLADAETELASQAALLSQTTAALLDEIEALRRTIGE
                     LQRGLVRIRMESARNLMTHAARTLRALRRATGVRVELRTLGEDTEFDKAVAEQLVDPI
                     TQLLRNAVAHAVEPPEERAARGKPQTATITIRARQDGNLLVLEISDDGRGVDTAALRD
                     RLVETSRWTQARAQLATDTDVLDALGTGVSVRGDADELAGRGIGLDLVRQTIARLGGE
                     VKVSSTPGRGTTFSLRLPLSTSLAQAMLFKVAGQVYAIPAVHVTETTIVEANAATATV
                     RGAQLPVLRLEQLLGYTAASERRPGVVVSFASKTMVCTVDKIVGPREIIIKPLGPLLA
                     PLTLYAAATISGSGKVQLILDPAQLVRRVYPDTADGPDPASTPMVLAGRALVVDDSRA
                     IREAMTSMLGREGWIVDVAEDGARALQMARQLKYDLVVTDLEMPELGGFDLIARLRKD
                     ERFKTTPMVIITSRANPEHRRRARDLGVRALVAKPITRRKLLEALAAR"
     gene            1693..4041
                     /locus_tag="VMZ53_25870"
     CDS             1693..4041
                     /locus_tag="VMZ53_25870"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31965.1"
                     /translation="MGRAVGLVVMLALAGCYEQPSETCKITCTPGAPNACPDGLECNP
                     AGLCNVGGASCKGPTVAFRSAGIGARHICGLSMAGDIYCWGDNSVGQVGTGTVGDPVT
                     LPTKLMGGPFTHLAVGAEHSCAMDGSGKPVCWGQNTYGQTRGAGPGTSPAPVETLLGQ
                     APAFDHLSAGGRATCALGNGQLWCWGFRDFTNGTAASQATRVAPVIDDWTDISCGFDH
                     CCGISTSMGALCFGENDNGQLGNSTMTTSAVPVKAGLPAGMTTLSIVAGHEVSCAIVS
                     AASTTEGELWCWGRNTNGTDIIDQANQNRLNPQRMGADADWTSISIGTGYACGQRGRR
                     VYCWGSSDHGGMGNGIWSNYNTPLAMAVDLGEADEVLLHTGSEADEVELACLRNGTDL
                     DCFGDNSYGELATGYTSRRATPVEITAPPMRSWKHVDAGHHHTCATLDDDSVYCWGST
                     EYGEANAGIKLGADAPCVDGEVCDRARPERMPAPLMRADVITAGDHFSCARDGTNAYC
                     WGIGARGYTGPSSSNPVHTVLGPGASSWVTLQGGDTVTCGLTSSNEYACWGYIPFNQP
                     TAMPLTLPGIQDITQIAFGENFGCVVGTNKVRTCWGTNANGELGYGDQANRNLSGAIT
                     VANIESIAADDRHACAVNTDGTVGCWGSNDYRESGSTAEGSAIISPVLVKRTGNTTLS
                     SCTQVSVGAQFSCAVCAGRVLCWGMDHEAELGRGRLYGGHTELAAEVTLPEDLTFIEM
                     SAGHHHSCAISTTGRMYCWGTSLHGQIGDGQQSRNLPTPVGR"
     gene            complement(4044..6782)
                     /gene="rnr"
                     /locus_tag="VMZ53_25875"
     CDS             complement(4044..6782)
                     /gene="rnr"
                     /locus_tag="VMZ53_25875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HUS31966.1"
                     /translation="MSHVERQAILDCLSRLVTPGIKLTALADDLGLRKQDYSGLRAQI
                     LELVEEGFVHVLPGGAFALAPQGRPADPHAKPTPPAPPPAKPVKLPKPAKAAKEVKRG
                     AALPWSKPPRPGPSPKVIEEYDQETGEIRIRDLTKPDRDLSTTGRHARIAAPLRETGR
                     HTRIVEPARRDDRDSRAPGSKLTGRITVHPAGYGFVETPEGTVFVPAKYRGNCLDGDE
                     VALDTWPGVRGTEGQVVEVIKRGRARLTGILRRVGRAVYLEPDDPRIAADFGRVGIDE
                     ALSGKDGDAVVVEITRYPDAARKELAGKVLKVLGDPEDPRTEIEKILACAVIPLEFPD
                     DALAQAKATSQELTAADLADRIDLRDRRFCTIDPETARDFDDALCIEDGPHGGPRVWV
                     AVADVGHYVRWNDALDREAAVRSVSVYLPDRVIPMLPKELSAGICSLNPLVDRCAMVV
                     RIDYSDDGDILDIGYMAAAIRSKARFDYPGVAAALEGDFRGRREQYRPWVPELTKLNN
                     LAQILRKKRQAKGALDLEISEPKVVLDADDPRLVRDVVKAKGDPAVKGAYQLVEEFMI
                     AANNAVGNFFMRRNAPTVWRVHAPPDPDRVTKLAELLGAYGIIVDVDEATTPLGMKAV
                     LDQIAQKPGAQALTFLLLRTLTQAVWDTVPIGHFGLASGEYVHFTSPIRRYPDLLAHR
                     LLKYHLHREGYASGGGYSKPPPEVETLAELAAASSKHERRAMEAERESVAMYRAYLMR
                     DQVGEEFPGTVSAVTSFGAFVEIEEPFVEGLIKLDSLGEPFEYDEIHMRLSGKRTGRT
                     IELGDKVLVEINNVSVIRRRIDFTLKATSSTKKGRVPRVEANARAPIPQRKKAGREVE
                     RAQLHQVERGAPKRGRAERGGRGAGGKLQLGVSHRGVAAGGGGGRGKKAGARRGKK"
     gene            complement(6799..8085)
                     /locus_tag="VMZ53_25880"
     CDS             complement(6799..8085)
                     /locus_tag="VMZ53_25880"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUS31967.1"
                     /translation="MNVRLAAVAAAAVLGCSGTQARQVGCSKDTDCRDPRVCDRGVCV
                     DPHPIAGVKAMSTTVTDDGGTEVSETSVKGPPAFAMAGGDARHTGRRSGPASTAAPKE
                     AWSVDVKGVVAGSPTIGPDGTIYVASHDGNLYAISDAGQIKWTFKTGDRSWSTPAIAD
                     DGTIYIGSDDDHLYAVKNDGSLKWKLRLGDCDPKGFGPESSRCDVDGGPTIGPDGTIY
                     VGGDGIHAVWADGTLRWKLATAEHVASTPALGNDGTVYAGSEDDALYAVGADGTKKWE
                     VRTGGDVDAMPIVGANGTIYVGSDDHTFYAITPQGSVAWRVITGADVRGGAALAADGT
                     LYVGSFDGRLYAIAPNGQVKWKLAAADKINSTPGIATNGTIVFGAQDEHVYAVAPDGT
                     LRWHIQLPGDVDSTPAIASNGTMYVAGDDGHLHAFR"
     gene            complement(8118..8393)
                     /locus_tag="VMZ53_25885"
     CDS             complement(8118..8393)
                     /locus_tag="VMZ53_25885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31968.1"
                     /translation="MAKLVDGFKDGKPELGRRTFLVPENHSEVLALLYHEQTGIVGGL
                     PVGPVTTDPFGAHGIRMYPDQDPVVLEETRRYFEKLAERFSNDLPKA"
     gene            8501..9793
                     /locus_tag="VMZ53_25890"
     CDS             8501..9793
                     /locus_tag="VMZ53_25890"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HUS31969.1"
                     /translation="MVLELWFDFSCPYAYLASRKAPHIARAAGVELVWKPMLLGGVFR
                     GIGAGDGPMATLGPAKARHNLQDMHRWAELFGAPFRMPAAHPMRTVRALRTLLALPHS
                     RWPAAIEALYAAYWQRGEDVTRDETIAGALRQSGLGDAEIAGALAAADNYKDELRART
                     DEAIALGIFGAPAWVIRRDGREPLLIWGQDRMHWVDAALAGWDPDASSPPGGPRPVSD
                     GVQPFRSPSTLDVYFDVSSPFAYLGLTQLPALANVTGVTPRLQPILLGGLFRDIGQVD
                     VPLFAMPPPKTRYTGLEMSRWARWWGVPFSMPTKFPQRTVTAQRLCVIAAEQSAAAGI
                     RLATTLGRAMWAEQRDLEDDATLRGLLDDAGLPREWVERTKEAAVKEALIARTAAAKQ
                     AGVFGVPAWIIDEKYLFWGQDRLELVMRALGGWRPEHG"
     gene            9790..10446
                     /locus_tag="VMZ53_25895"
     CDS             9790..10446
                     /locus_tag="VMZ53_25895"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HUS31970.1"
                     /translation="MIYVATIVCAVACLVLVGAEYKQAAMLRIVSKCVASAAFIAVGY
                     LAAHEDRSYERHILLGLVLGAVGDVALLGRSNRAFMAGLVAFLLGHLAYVIACIHTVD
                     PHETLGLAGLYAVAPAIVGLGALAYLWPRLGKMRVPVILYVAVIISMVVAAIAVGRSE
                     TLPLHARERLVIGAVLFFISDLAVARDKFVAKGFSNRAWGLPTYYAGQLFIAWSLASS
                     "
     gene            complement(10447..10602)
                     /locus_tag="VMZ53_25900"
     CDS             complement(10447..10602)
                     /locus_tag="VMZ53_25900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31971.1"
                     /translation="MGRTKRSVKSRPKYKQKRRMKDRRMKAKKAAAHKRGLNRNRKRR
                     SKRGSKR"
     gene            10676..11305
                     /locus_tag="VMZ53_25905"
     CDS             10676..11305
                     /locus_tag="VMZ53_25905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS31972.1"
                     /translation="MADLKQLMARYQDGDASAFRELYAQVAPRLLGYLLKMARSRALA
                     DDLLQQTFLKVHRARAAYIRDSDPLPWIYSIAHRTFIDEARKKKRAIVNVASDPEGTV
                     PDVPATLTGDAVGAADEPVDSEETKAALDALAQLPEQQREAVVLTKLEGKSVAEAAEI
                     AGTTVGAMKVRAHRGYEALRKLLGAAPARAKKMASALLVLVSAAGWRTA"
     gene            11624..>12011
                     /locus_tag="VMZ53_25910"
     CDS             11624..>12011
                     /locus_tag="VMZ53_25910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31973.1"
                     /translation="MADAIDDLAKARPPSPRAMSAELEAELGRIEAVKPRRPARQVAT
                     LVGISLVYGAGLLAALSMRRDMTELPTAWIVAAAVAWFCGFVVPSYLALVPKSGSMMP
                     RWQLGAAAAIVASIAFIVLGLVVHPSG"
BASE COUNT         1940 a         3981 c         4035 g         2055 t
ORIGIN      
        1 gtggtgttgc agagccgaag tcgtacttgc gcgtcgagcc cgagcgcgtc gatgccctga
       61 tgtcgagtgc gggtgagctg ctgttcgatc gcacgcgtat cgaacgtcgc gttcaactgc
      121 tgcgcacgct cgcccgagac ctcgcccgca cgcgacaaac gctcagcgac gcaatcgcgc
      181 gcgaagcccc tggcaaagcg tcgctcgccg acgccgaaac cgagctcgca agccaagccg
      241 ccctcctctc gcaaacgacc gccgccctcc tcgacgagat cgaagcgttg cgccgcacga
      301 tcggcgagct ccaacgcggc ctcgtgcgca tccgcatgga gagcgcgcgc aatctcatga
      361 cgcacgcggc acgcacgctg cgagcactgc ggcgcgcgac cggtgtgcgc gtcgagctgc
      421 gcacgctcgg agaagacacc gaattcgaca aggccgtcgc cgaacagctc gtcgatccga
      481 tcacgcagct gcttcgcaac gcagtggccc atgccgtcga gccgcccgaa gagcgcgccg
      541 cgcgcggcaa gccacagacc gccacgatca cgattcgcgc tcgccaggac ggcaacttgc
      601 tcgtgctcga gatctccgac gacggccgcg gcgtcgatac ggctgcgctc cgcgatcgcc
      661 tcgtcgagac gagccggtgg acacaagcgc gcgcgcagct cgcgaccgac accgatgtgc
      721 tcgatgcgct cggcacgggc gtgtcggtgc gcggcgatgc ggacgagctc gctgggcgcg
      781 gcatcggcct cgatctcgtg cgccagacga tcgcgcgcct cggcggcgaa gtgaaagtgt
      841 cgtcgacgcc aggccgcggc acgacgttca gcttgcggct gccgctgtcg acctcgctcg
      901 cgcaagccat gctgttcaag gtcgccggtc aggtctacgc gattcccgcc gtgcacgtca
      961 cggagaccac gatcgtcgaa gcgaacgcgg cgaccgcgac ggttcgcggc gcgcagctcc
     1021 ccgtgctccg gctcgagcag ctgctcggct acacggcagc gagcgagcgg cggccaggag
     1081 tcgtcgtctc gttcgcgagc aagacgatgg tctgcaccgt cgacaagatc gtcgggcctc
     1141 gcgagatcat catcaagccg ctggggcctc tgctcgcgcc actcacgctc tatgcggctg
     1201 cgacgatcag cggttcgggc aaggtgcagc tgatcctcga tccggcgcag ctcgtgcgtc
     1261 gcgtctatcc ggatacggcg gacggtccgg atcccgcgtc gacgccgatg gtgctcgcgg
     1321 gccgtgcgct cgtcgtcgac gattcccgcg cgattcgcga ggcgatgaca tcgatgctcg
     1381 gccgcgaggg ctggatcgtc gacgtcgcgg aggacggcgc gcgggcgctc cagatggcgc
     1441 ggcagctcaa gtacgacctc gtcgtcaccg acctggagat gcccgagctc ggaggcttcg
     1501 acctgatcgc gcggctgcgc aaggacgagc gattcaagac gacaccgatg gtgatcatca
     1561 cgtcgcgcgc gaacccggag caccgccgcc gcgctcgaga cctcggcgtc cgggcgctcg
     1621 ttgccaagcc gatcacgcga cgcaagctgc tcgaagcact cgcggcccgc tgatttccgt
     1681 taccatggtg acgtgggtcg agctgtaggt ctcgtggtga tgctcgcgct ggcgggctgc
     1741 tacgagcagc cgtcggagac gtgcaagatc acctgcacgc cgggcgcgcc gaacgcatgc
     1801 ccggatgggc tcgagtgcaa tcccgccggc ctatgcaacg tcggtggtgc atcgtgcaag
     1861 ggaccgacgg tcgcgttccg tagcgctggc atcggtgcgc gccacatctg cggcttgtcc
     1921 atggccggcg acatctactg ctggggcgac aactccgtcg gccaggtcgg cactggaacc
     1981 gtcggcgacc cggtcaccct tcccacgaag ctgatgggcg gaccgttcac gcatctcgcc
     2041 gtcggcgccg agcattcgtg cgcgatggat ggcagcggca agccggtgtg ctggggccag
     2101 aacacgtacg gtcagacgcg aggcgcaggt cctggcacga gccccgcgcc cgtcgagacg
     2161 ctgctcggtc aggcgccggc cttcgatcac ttgagcgcgg gtggtcgagc gacctgcgcc
     2221 ctcggcaacg gtcagctctg gtgttggggc ttcagagact tcacgaacgg caccgccgca
     2281 tcacaagcca cgcgcgtcgc tccggtcatc gacgattgga cagacatctc gtgcggcttc
     2341 gatcattgtt gcggcatcag cacatcgatg ggcgcgctct gcttcggcga gaacgacaat
     2401 ggccagctcg gcaactcgac gatgacgacg agcgcggtgc ccgtgaaggc cggtctgccc
     2461 gcgggcatga cgacgctgtc gatcgtcgcc ggccacgagg tgtcgtgtgc gatcgtgtcg
     2521 gccgcctcga cgacagaagg cgagctgtgg tgctgggggc gcaacacgaa tggcaccgac
     2581 atcatcgatc aagcaaacca gaatcggctc aacccacagc gcatgggcgc cgatgcggac
     2641 tggacctcga tctcgatcgg gaccggctac gcgtgcggcc aacgcggtcg ccgcgtgtac
     2701 tgctggggaa gcagcgacca cggcggaatg ggcaacggaa tctggagcaa ctacaacacg
     2761 ccgctcgcga tggcggtcga tctcggcgaa gccgacgaag ttctgctcca cacaggctcc
     2821 gaggctgacg aggtcgagct cgcctgcttg cgcaacggca cggacctcga ctgcttcggc
     2881 gacaactcgt acggcgagct cgcaaccggc tacacatcgc gacgagcgac gcccgtcgag
     2941 atcacggcac ctccgatgcg atcgtggaag cacgtcgacg cgggccatca ccacacgtgc
     3001 gcgacgctcg acgacgacag cgtctattgc tggggctcga ccgagtacgg cgaggcgaac
     3061 gccgggatca agctcggtgc cgatgcgccg tgcgtcgacg gcgaggtctg tgatcgcgcg
     3121 cgaccggagc gaatgcccgc gccgctgatg cgcgccgatg tgatcaccgc aggcgatcat
     3181 ttctcgtgcg cgcgcgacgg cacgaatgcc tactgttggg gcatcggcgc tcgagggtac
     3241 acggggccat cgtcgagcaa ccctgttcac acggtgctcg gtcctggcgc gagctcgtgg
     3301 gtgacgcttc agggtggcga cacggtgacg tgcggtctca cgtcgtcgaa cgagtacgcg
     3361 tgctgggggt acatcccgtt taaccaaccg acagcgatgc cgctgacgct tcctggtatt
     3421 caggacatca cgcaaatcgc gttcggcgag aacttcggct gcgtcgtcgg tacgaacaag
     3481 gtacgcacgt gctggggaac taacgccaat ggtgagctcg gctatgggga ccaagcgaat
     3541 cgcaatctga gcggcgcgat tacggtcgcc aacatcgagt cgatcgcagc cgacgaccgg
     3601 cacgcatgcg ccgtcaatac cgacggcacc gtcggatgct ggggctcgaa cgactaccgc
     3661 gaatcaggct cgactgccga gggttctgcg atcatctcgc cggttctcgt caagcgcacc
     3721 ggcaatacga cgttgagcag ctgtacgcag gtctcggtcg gcgcgcagtt cagctgtgcg
     3781 gtgtgtgccg gtcgcgtgct gtgttggggc atggaccacg aggcggagct cgggcgcggg
     3841 cgtctctatg gtggccacac cgagctcgcc gccgaggtga cgctgccgga ggacctgacc
     3901 ttcatcgaga tgtcggccgg tcatcaccac agctgcgcga tcagcacgac gggtcgcatg
     3961 tactgctggg gcacgagctt gcacggccag atcggcgacg gtcagcagag tcgtaatctg
     4021 ccgacgccgg tcggtcgcta gctctacttc ttgccgcgcc gcgcgccggc ttttttgccg
     4081 cgaccaccgc cgccgcccgc tgcgacgccg cgatgcgaga cgccgagctg cagcttgccg
     4141 ccggcaccgc gtccgccgcg ctctgcacgt ccgcgcttgg gcgcgccgcg ctcgacctga
     4201 tgcagttgcg cgcgctcgac ctcgcgtccg gctttcttgc gctgcgggat cggtgcacga
     4261 gcatttgcct cgacgcgcgg cacgcggccc ttcttcgtcg aagaagtcgc cttgagcgta
     4321 aagtcgatgc gacggcgaat caccgaaacg ttgttgatct cgacgagcac tttgtcgccg
     4381 agctcgatcg tgcggccggt gcgcttgccc gacagccgca tgtgaatctc gtcgtattcg
     4441 aagggctcgc cgagtgaatc gagcttgatg agaccttcga caaacggctc ttcgatttcg
     4501 acgaacgcgc cgaagctcgt aacggccgag accgtgcccg ggaattcctc gccgacctga
     4561 tcgcgcatga ggtacgcgcg atacatcgca accgattcgc gctcggcctc catcgcacga
     4621 cgctcgtgct tactcgatgc ggccgcgagc tccgcgagtg tctcgacctc cggaggcggc
     4681 ttcgagtacc cgccgcccga ggcgtagccc tcgcgatgca ggtggtactt gagcaggcga
     4741 tgcgcgagca gatccggata gcgccggatc ggcgacgtga agtgcacgta ctcgccggaa
     4801 gcgagaccga agtgaccgat cgggacggtg tcccagacgg cctgcgtgag cgttcgcagc
     4861 agcaagaacg tcagcgcctg cgcgccgggc ttctgcgcga tctgatcgag caccgccttc
     4921 atgccgagcg gcgtcgtggc ttcgtcgacg tcgacgataa ttccgtaggc gccgagaagc
     4981 tccgcgagct tcgtaacgcg atccggatcg ggcggcgcgt gcacacgcca caccgtcgga
     5041 gcgttgcggc gcatgaagaa gttaccgacc gcgttgttcg ccgcgatcat gaactcttcg
     5101 acgagctgat acgcgccctt caccgccggg tcgcccttcg ccttgaccac atctcgcacg
     5161 aggcgcggat cgtcggcgtc gagcacgact ttgggctcgg agatctcgag atcgagcgcg
     5221 cccttcgctt gccgcttctt gcgcagaatc tgagcgaggt tgttgagctt cgtgagctcg
     5281 ggcacccagg ggcggtactg ttcgcgacga ccgcggaagt cgccttcgag cgcggcagcg
     5341 acgcccggat aatcgaagcg cgctttgctg cgaatcgcgg cggccatgta gccgatgtcg
     5401 aggatgtcgc cgtcgtcgct gtagtcgatg cgcacgacca tcgcgcaacg atcgacgagc
     5461 gggttgagcg agcagatgcc cgccgacagc tccttcggca gcatcgggat cacgcgatcg
     5521 ggcaagtaca ccgagacgct gcgcaccgcg gcctcacgat cgagtgcgtc gttccagcgc
     5581 acgtaatgac cgacgtccgc gaccgcgacc cacacgcgtg gaccaccatg cgggccgtcc
     5641 tcgatgcaca gtgcatcgtc gaagtcgcgc gctgtttcgg gatcgatggt gcagaaccgg
     5701 cgatcgcgca gatcgatacg atcggcaagg tcagcggcgg tcagctcctg cgatgtcgcc
     5761 ttcgcttgcg cgagtgcgtc gtcgggaaac tcgagcggga tgaccgcgca cgcgaggatc
     5821 ttctcgatct cagtccgtgg atcttccggg tcgccgagca ccttcagcac cttgccggcg
     5881 agctctttgc gcgcagcgtc gggatagcgc gtgatctcga cgacgacggc atcgccgtct
     5941 ttgccggaca gcgcctcgtc gatgccaacg cgaccgaagt cggcggcgat gcgaggatca
     6001 tcaggctcga gatacaccgc gcgaccgacg cggcgcagga tgccggtcaa ccgtgcacgg
     6061 ccgcgcttga tgacctcgac gacctgccct tccgtgccgc gcacgccggg ccatgtatcg
     6121 agggcgacct cgtcaccatc gaggcagttg ccgcgatatt tcgcgggaac gaagacggtg
     6181 ccctctggcg tctcgacgaa gccatagccc gcggggtgca ccgtgatgcg gccggtgagc
     6241 ttgctgccgg gggcgcgcga gtcgcgatcg tcacgccgag ccggttcgac gatgcgcgtg
     6301 tggcggcccg tctcgcgcag cggcgccgcg atgcgagcgt gcctgcccgt cgtcgagaga
     6361 tcgcgatcgg gcttcgtcag atcgcggatc cggatctcgc ccgtctcctg atcgtattcc
     6421 tcgatcacct tcggcgacgg accggggcga ggcggtttgg accacggcag cgcggcgccg
     6481 cgcttcactt ctttggcggc tttcgccggc tttggcagct tcacgggttt ggctggtggt
     6541 ggtgcaggtg gcgtgggttt ggcgtgcgga tcggcgggac gaccttgggg cgcgagcgcg
     6601 aatgcgccgc cggggaggac gtgcacgaag ccttcttcga cgagctcgag aatctgcgcg
     6661 cgaaggccgc tgtaatcttg cttgcgcagg ccgaggtcat cggcgagggc ggtgagcttg
     6721 atgcccgggg tgaccaggcg tgacaggcaa tcgaggatcg cctgtcgttc gacatgagac
     6781 attttgttct ttttctagtt agcgaaaagc atggaggtgc ccgtcgtcgc cggcaacata
     6841 catcgtgccg tttgaagcga tggcgggcgt tgaatcgaca tcgcctggaa gctggatatg
     6901 ccagcgaagc gtgccgtcgg gagcgactgc atagacgtgc tcgtcttgcg caccaaaaac
     6961 gatggtgccg ttcgtcgcga tcccaggagt cgagttgatc ttgtcggccg cagcgagctt
     7021 ccacttcacc tggccgttcg gtgcaatggc gtagagccgt ccgtcgaagc tgccgacgta
     7081 gagggtgccg tcggcggcga gcgctgcccc accgcgaaca tcagcgccgg tgatcacgcg
     7141 ccacgcgacg gagccttgcg gcgtgatcgc atagaacgtg tgatcgtcgc tgccgacgta
     7201 gatggtgccg ttcgcgccga caatcggcat cgcatcgacg tcgccgccgg tgcgcacttc
     7261 ccacttcttg gtgccgtcgg cgccgaccgc gtagagcgca tcgtcctcgc tgcccgcata
     7321 gacggtgccg tcgttgccga gcgcgggcgt cgacgcgacg tgctcggcgg tggcgagctt
     7381 ccagcgcagc gtgccatcgg cccacaccgc gtgaatgccg tcgccgccga catagatcgt
     7441 gccgtcggga ccgatcgtgg gcccgccgtc gacatcgcag cgcgatgact cgggaccaaa
     7501 gcccttcgga tcacaatcgc cgaggcgcag cttccacttc agcgagccgt cgttcttcac
     7561 ggcgtagaga tgatcgtcgt cggagccgat gtagatcgtg ccgtcgtcgg cgatcgcagg
     7621 tgtcgaccac gagcggtcgc cggtcttgaa cgtccacttg atctgaccgg cgtcgctgat
     7681 cgcatagaga ttgccgtcgt gcgacgcgac gtagatcgtg ccgtcgggac cgatcgtcgg
     7741 tgagcctgcg acgacaccct tgacgtcgac gctccatgct tccttcggtg cggccgttga
     7801 cgcgggcccg ctgcgacgac cggtgtggcg cgcatcgccg cctgccattg cgaacgcggg
     7861 cggtcccttc acggacgtct cgctgacctc cgtgccgcca tcgtccgtga cggtcgtgga
     7921 catcgccttc acgcccgcga tcggatgcgg atcgacgcag acaccacggt cgcagacccg
     7981 cggatcgcga caatccgtgt ccttcgagca cccgacttgg cgggcttgcg tgcccgagca
     8041 accgagcact gcggcagctg ctacagccgc caacctaacg ttcacgcgcg aaacctaacc
     8101 ctggaatgta gaagccacta tgccttcgga agatcgttcg agaatcgctc ggcgagcttc
     8161 tcgaagtacc gacgtgtttc ttctagcacg accggatcct gatcgggata catgcgaatg
     8221 ccgtgcgccc caaacgggtc cgtcgtcacg gggcccacgg gaagaccacc aacgattcca
     8281 gtctgctcgt gatagagcag cgcgagcacc tcggagtgat tttcgggcac caagaaagta
     8341 cggcgcccta gctcgggctt gccgtcttta aacccgtcga ccagcttggc catgttcgac
     8401 ccaaccagtc taccgtcacg aggttcttcg ggaaagtccg tttggcacgt gtgatcgcac
     8461 tgcctcccac acatgaacac ccggggggta gtctcacgcc gtggttctag aactgtggtt
     8521 cgacttctcg tgcccgtatg cgtatctcgc gtcgcgcaag gcgccgcata tcgctcgggc
     8581 ggcgggggtg gagctcgtct ggaaaccgat gctgctcggc ggcgtctttc gtgggatcgg
     8641 cgccggtgac ggtccgatgg cgacgctggg accggccaag gcgcgccaca acctccagga
     8701 tatgcaccgc tgggccgagc tgttcggcgc gccgtttcgc atgcctgctg cacacccgat
     8761 gcgtactgtt cgggcgctcc gtacgctgct cgcccttccc cactcccgct ggcctgccgc
     8821 gatcgaggcg ctgtatgcag cgtactggca gcgcggcgaa gacgtcacgc gtgacgagac
     8881 gatcgccggt gcgctccgcc aatcgggcct cggcgatgcg gagatcgcgg gcgcgctcgc
     8941 agccgccgac aattacaaag acgagctccg cgcgcgcacc gatgaagcga tcgcgctcgg
     9001 catcttcggc gcaccggcgt gggtgatccg gcgcgatggt cgcgagccgc tgctgatctg
     9061 gggacaagat cgaatgcact gggtcgatgc cgcgctcgcg ggctgggatc ccgatgcgtc
     9121 gtcgccgccc ggtggcccac ggcccgtgag tgatggcgtg cagccgtttc gttcgccgtc
     9181 gacgctcgac gtgtacttcg acgtgtcgag cccgtttgcg tatctcgggc tgacgcagct
     9241 gccggcgctc gccaacgtga cgggcgtgac gccgcgtttg cagccgatcc tcctgggcgg
     9301 attgtttcgc gacatcggcc aagtcgacgt tccgctgttc gcgatgccac cgccaaagac
     9361 tcgatacacg ggtctcgaga tgtcgcggtg ggctcgctgg tggggcgtgc cgttctcgat
     9421 gccgacgaag tttccgcagc gaacggtgac cgcgcagcgc ctgtgcgtga tcgcggcgga
     9481 gcagtccgcg gccgccggca tccgactcgc gacgacgctc ggtcgcgcga tgtgggcgga
     9541 gcaacgcgat ctcgaagacg acgcgacgct gcgcggcctc ctcgacgatg cgggcctccc
     9601 gcgcgagtgg gtcgagcgaa cgaaagaggc ggcggtgaaa gaagcgctga tcgcgcgcac
     9661 ggcggcggcg aaacaagcag gcgtgttcgg cgtcccggcg tggatcatcg acgagaagta
     9721 tctcttctgg ggccaagacc ggctcgagct cgtgatgcgc gcgctcggcg gctggcgacc
     9781 ggagcacgga tgatctacgt cgccacgatc gtctgcgcag tcgcgtgtct cgtcctcgtc
     9841 ggcgccgagt acaagcaagc agcgatgctg cgcatcgtca gcaagtgcgt cgcgtcggcg
     9901 gcgttcattg cggtcggcta tctcgcggcg cacgaggatc gcagctacga gcgacacatc
     9961 ttgctcggcc tcgtgctcgg cgccgtcggc gacgtcgcgc tgctcggccg cagcaatcgc
    10021 gcattcatgg cggggctcgt cgcattcctg ctcggccacc tcgcctacgt gatcgcgtgc
    10081 attcacaccg tcgatccgca cgagacgctc gggctcgccg gtttgtacgc cgtcgccccg
    10141 gccatcgtcg gcctcggcgc tctcgcctat ctctggccgc ggctcggcaa gatgcgcgtg
    10201 ccggtgatcc tctacgtcgc ggtcatcatc agcatggtag tcgcggcgat cgccgtcggc
    10261 cgcagcgaga cactgccgct gcacgcgcgc gagcgtctgg tgatcggcgc ggtgctgttc
    10321 ttcatcagcg acctcgccgt cgcacgcgac aagttcgtcg cgaaaggctt ctcgaaccgc
    10381 gcgtggggcc taccgacgta ctacgccggc cagctcttca tcgcgtggtc gctcgccagc
    10441 agctgatcag cgcttgctgc cgcgcttgct acgacgcttg cggttccggt tgagaccgcg
    10501 cttgtgcgcc gccgccttct tcgccttcat gcggcgatcc ttcatgcggc gcttctgctt
    10561 gtacttcgga cgtgatttga cggagcgctt agtacgaccc atgtccgcgt ctatacatcg
    10621 gcctcgggac ctgtaaccag ggggcgcggc cgtcgtatat ccctctgcgg tcgaaatggc
    10681 cgatctcaag cagctcatgg cgcggtacca agatggcgac gccagcgcgt tcagagagct
    10741 gtacgcgcag gtggccccgc gcctgctcgg ctatctcctc aagatggcgc ggtcacgggc
    10801 gctcgccgac gacctcctgc aacagacctt cctgaaggtg catcgcgcgc gggccgccta
    10861 tatccgcgac tccgatcccc tcccgtggat ctactcgatc gcccaccgca cgttcatcga
    10921 cgaagcccgc aagaagaagc gcgcgatcgt caacgtggcc agcgaccccg agggcaccgt
    10981 ccccgacgtc cccgcaacgc tcaccgggga tgcggtcggt gccgccgacg aaccggtcga
    11041 cagcgaggaa acgaaagccg cgctcgacgc gctcgcccag ctgcccgagc aacaacgcga
    11101 ggcagtcgtg ctgacgaagc tcgagggcaa gtccgtcgcc gaggccgccg aaatcgcggg
    11161 cacgacggtc ggtgcaatga aagtccgcgc gcaccgcggc tacgaggcgc tccgcaagct
    11221 gctcggcgct gcgcctgcgc gtgcgaagaa aatggcgtcc gcgctcctcg tgctcgtcag
    11281 cgccgctggt tggaggaccg cgtgatggcg ctccaccgac cactcgcgat acgcgactgc
    11341 gctctggagt ccgcggtgat cccagaggga acgcaaggga gcaaccagag caagggacgc
    11401 aagccggagg atcagtgggg agcgatcgtg ctctgggaga acgtgtgtcg cgagcgcgcg
    11461 gcgcgccgta ccggctcgct gaagccggag caggatcatc gtgcgctcgt gctcacgacc
    11521 agcgcgtgtc acgagcgcgg cgcgcgcctt gacccttctc tgctcttctt gcgtccctct
    11581 ccggccttgc ccccttgcgt tccatccggg acggaggcgc gctgtggctg acgctatcga
    11641 tgacctcgcg aaggcgaggc cgccgagccc tcgggcgatg tcggcggagc tcgaggcgga
    11701 gctggggcga atcgaggcgg tgaagccgcg aaggcccgcg cgacaggtcg ccacactcgt
    11761 cggaatctcg ctcgtgtacg gtgcgggact tctcgcggcg ctcagcatgc ggcgcgatat
    11821 gacggagctg ccgacggcgt ggatcgttgc ggcggcggtc gcgtggttct gcggcttcgt
    11881 cgtgccgagc tatttggcgc tcgtgccgaa gagcggttcg atgatgccgc gttggcaact
    11941 cggcgcagcg gcggcgatcg tcgcgtcgat cgcattcatc gtgctcgggt tggtcgtgca
    12001 tccgtccggg c
//
LOCUS       DATLBZ010000513         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4364683, whole genome shotgun sequence.
ACCESSION   DATLBZ010000513 DATLBZ010000000
VERSION     DATLBZ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4364683"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..693)
                     /gene="pdxH"
                     /locus_tag="VMZ53_25915"
     CDS             complement(37..693)
                     /gene="pdxH"
                     /locus_tag="VMZ53_25915"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HUS31974.1"
                     /translation="MQPLWRLGEQYQGPLLDPASCDPDPFVEVQRWLTVAIDGGIPSA
                     NAMTLATVDERGRPAARIVLLKELDQRGFVFFTSYDGRKARDMTAHAHAALVLWWEPL
                     HRQIRVEGRVEKLAAAESDAYFATRPRGSQLGAIASPQSQVLASRQELEAKVAALQAQ
                     YSAEAPPRPANWGGYRVVPDMIELWQGQPSRLHDRVRYTLVASPGTTPATWTRDRLAP
                     "
     gene            complement(756..1250)
                     /locus_tag="VMZ53_25920"
     CDS             complement(756..1250)
                     /locus_tag="VMZ53_25920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31975.1"
                     /translation="MRRIFVAALVFAAACGGKSAAPAKVGEGSGTGAGSGSGPMMGHQ
                     GEHDDMSPELKRFRDVFAPLWHAEKGAKRTADTCAAVPELTASADAIGKATPPPSAMA
                     DTWTKGTRGLVAAVAKLGEACKANDATKFEAAFTDVHESFHALMEMAHMHDSGSGAEH
                     DHMQ"
     gene            1309..1815
                     /locus_tag="VMZ53_25925"
     CDS             1309..1815
                     /locus_tag="VMZ53_25925"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HUS31976.1"
                     /translation="MHLFVVRHAVAEDAEPGQDDAARELTSEGARKFKRSVQGLRELR
                     WTFDRVLTSPWKRAVQTAELLAPVTDGQTISTELLCDKPRPELFALIAETTAPHVEGD
                     ARQATAIVGHEPWLSELVGWLAFGDPHHGEAIELKKGGVVWLEGHAIPGGMKLRAVLP
                     PKVLRELR"
     gene            complement(1812..2639)
                     /locus_tag="VMZ53_25930"
     CDS             complement(1812..2639)
                     /locus_tag="VMZ53_25930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31977.1"
                     /translation="MRWASILVLLAACRFGFEARTGELDDADSDVTTGDSGPVPTCAG
                     HDEDADHFPDACDSCPTVPNGAQVDGDGDGVGDACDPRPATGGDYIQLFDAHTSSTSG
                     TYYVLNSQYSWQGDALRLGLNTDVGQADFVLTALPSRIATRMHVVTTQSMTLQWFGVW
                     YSNNMADTKKVFAEIERDPPVAMTAYDLHEASMGSATRYSAFTFGAPTFSVGDTIDLT
                     IDTSLVTGGDDVMTVIDPQGVQRTHALAITIPRDVYGFLEAQSNTIDFDYLIAYGIR"
BASE COUNT          446 a          917 c          954 g          490 t
ORIGIN      
        1 agcgggcgcg acgcgggttc gcggtcagcg gcgcggtcag ggcgcgaggc ggtcgcgcgt
       61 ccatgttgct ggcgtggtcc ccggcgacgc gaccagcgtg tagcgcactc gatcgtgcag
      121 ccgcgacggc tggccctgcc acagctcgat catgtccggt acgacgcggt agccgcccca
      181 gttagcggga cgcggcggcg cctcggcgct gtactgcgcc tgtagagcag cgaccttcgc
      241 ctcgagctct tgccgcgacg cgagcacctg cgattgcggc gatgcgatcg cgccgagctg
      301 cgagccgcgc ggtcgcgtgg cgaaatatgc gtcggattcg gcggcggcaa gcttctcgac
      361 gcgaccttcg acgcggatct ggcggtgcag cggctcccac cagaggacga gcgcggcgtg
      421 cgcgtgcgcg gtcatgtcgc gcgctttgcg tccgtcgtac gacgtgaaga acacgaagcc
      481 gcgctggtcg agctccttga gcagcacgat gcgagctgcg ggtcggcctc gttcgtcgac
      541 ggtcgctagc gtcatcgcgt tcgcggatgg gatgccgcca tcgatcgcga cggtgagcca
      601 gcgctgcact tcgacgaacg gatctggatc gcagctcgcc ggatcgagaa gcgggccctg
      661 atactgctcg ccgaggcgcc agagtggctg catgacttcg gtctaccacg ccctgtgcgc
      721 gagccgcgtc tgtcccagcg gctggctggc gactgtcact gcatgtggtc gtgctcggcg
      781 ccgctgccgg aatcgtgcat gtgcgccatc tccatcagcg cgtggaacga ttcgtggacg
      841 tcggtgaatg cggcttcgaa cttggtcgcg tcgttggctt tgcatgcctc gccgagcttg
      901 gcgaccgcgg cgacgagacc gcgcgtgcct ttcgtccacg tatcggccat cgcagacggt
      961 ggcggcgtcg ccttgccgat cgcgtcggcg ctcgcggtga gctccgggac cgcggcgcac
     1021 gtatcggcgg tccgcttcgc gcccttctcg gcgtgccaca gcggcgcgaa cacgtcgcgg
     1081 aacctcttca gctcgggaga catgtcatcg tgctcgccct ggtggcccat catcgggcca
     1141 gagccggagc cagcgcccgt tccagagcct tcaccgacct ttgcgggagc ggcggacttc
     1201 ccaccgcacg cagcggcgaa caccaatgcg gcaacaaaga tgcgtctcat gccgcggctt
     1261 tcgcacgcgc tcgctcgctc tgtcaacgcg caggggtaga gtcgagacat gcatctcttt
     1321 gttgtgcgcc acgcagtcgc cgaggatgcg gaacctggcc aggacgatgc ggcgcgcgag
     1381 ctcacgagcg agggtgcgcg gaagttcaag cgctccgtgc aggggttgcg cgagctgcgt
     1441 tggacgttcg accgcgtgtt gacgagcccg tggaagcgcg ctgtccaaac ggcggagctg
     1501 ctcgcgcccg tcaccgatgg tcagacgatc tcgaccgagc tgctctgtga caagccacgg
     1561 ccggagctgt tcgcgttgat cgcggagacg accgcgccgc acgtcgaagg cgatgcacga
     1621 caagcgaccg ccattgtcgg ccacgagcca tggctctccg agctcgtcgg ttggctcgcg
     1681 tttggcgacc cgcatcatgg cgaagcgatc gagctcaaga agggcggcgt cgtgtggctc
     1741 gagggccacg cgattccagg cggcatgaag ctacgcgcgg tgctgccgcc gaaagtcttg
     1801 cgcgagctgc gctaacgaat gccgtaggcg atcaagtagt cgaagtcgat cgtattgctc
     1861 tgcgcttcga ggaaaccgta gacgtcgcgt gggatcgtga tcgcgagcgc atgcgttcgc
     1921 tgcactccct gcggatcgat gactgtcatc acgtcgtcgc cgccggtcac gagcgacgta
     1981 tcgatagtga gatcgatcgt atcgcctaca ctgaacgtcg gtgcgccgaa cgtgaacgcg
     2041 ctgtagcgcg tcgccgaacc catgctcgcc tcgtgcagat cataggcggt catcgcgaca
     2101 ggcggatcgc gctcgatctc ggcgaacacc ttcttcgtgt cggccatgtt gttgctgtac
     2161 caaacgccga accattgaag cgtcatcgat tgcgtcgtga cgacgtgcat ccgcgtcgcg
     2221 attcggctcg gcagcgccgt caggacgaag tccgcttggc cgacatcggt attcaaaccg
     2281 aggcggagcg cgtcgccttg ccacgagtac tgcgaattca gcacgtagta cgtgcccgac
     2341 gtgctgctcg tgtgcgcgtc gaagagctgg atgtagtcgc cgcccgtcgc cggccgcgga
     2401 tcgcacgcgt caccaacgcc gtctccgtca ccgtcgacct gcgcaccatt cggaaccgtc
     2461 gggcagctgt cgcaagcatc gggaaaatga tcggcgtcct cgtcgtgacc cgcgcacgtg
     2521 gggaccgggc cgctgtcgcc cgtcgtgaca tcggagtccg cgtcgtcgag ctcgccggtg
     2581 cgcgcctcga agccgaagcg acacgcagcg agcagcacga ggatcgacgc ccacctcaca
     2641 ctggatagct tatcgcaacg ggcgcatcgc tttggcgcga cgtagatcgt tgaccgctcg
     2701 cccgacgctg tcgtcgtgaa ggagcagctt ggagagcgcc cccgtcgaga ttccgaggcg
     2761 ctgcgccgtc gtgccaatct cgaggttgcc cgcgacgagc aaatcga
//
LOCUS       DATLBZ010000514         7461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5874052, whole genome shotgun sequence.
ACCESSION   DATLBZ010000514 DATLBZ010000000
VERSION     DATLBZ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5874052"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VMZ53_25935"
     CDS             complement(<1..1412)
                     /locus_tag="VMZ53_25935"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HUS31978.1"
                     /translation="MSFFPIDRVMPEALRAGKIRGVHLAIVVDNTEGNGNTIGYQIKV
                     KYPWMNDQDATYWARIAIPMAGKERGTYFLPEKDDQVLVVFEHGDINRPIMIGALWSK
                     KQEPVEVNQSGKNNTKMIKSRAGHRIIFDDKDGSEKITIVDKTKKNKIVLDSPGKLVQ
                     IECAGDIEVKAADNIIMHSKTLKVGTTEGIKGSAQSVLSHAGATFGVKAQGVTVNGAS
                     VQINVSNSPATNVSGSGKGELGGAAVEKAKDQIEAKAKPASGGGGGGGGGGGGGGGGS
                     GGASGGASGSASGSASGSASGGASGGASGSASGSASGGASGSASGGASGSASGGASGG
                     AKGGASGSASGSASGSASGGASGSASGSASGSASGGASGKADAGAQGGASGNASGSAS
                     GSAGAGAQGGASGGAQGGAAGGASGKADAGASGGAQGGAAGGASGKADAGAQGGASGS
                     ASGSASGSAGAGAQGGASGGA"
     gene            complement(1409..2098)
                     /locus_tag="VMZ53_25940"
     CDS             complement(1409..2098)
                     /locus_tag="VMZ53_25940"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HUS31979.1"
                     /translation="MDNLLLGLHYGVVCQNKDPENLDRVKIRLPWLDEGDTDQTHWAQ
                     LVTPMEGQKFGWYTLPDVDDVVVVAFIGGDTAQPIILGGVWSTPDFSPEPNEDGKNNF
                     RGFRSRVGHRFVFDDSDKTKVYFADKSTKLMVGVGNFAKAGAGPNVCAVHQPPMSGDK
                     GVSISSMEGNLEITCKDGVLKMTAGKNVKINAQDTIDIKAGQDIKMDGSSAKLTSQDN
                     SNYDASTIDIA"
     gene            complement(2129..3196)
                     /locus_tag="VMZ53_25945"
     CDS             complement(2129..3196)
                     /locus_tag="VMZ53_25945"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HUS31980.1"
                     /translation="MGAQTNFKIKIGKLEIESADVQGFSIERDMFQPDMCAIVLSNQE
                     NNYSPKIEMAGPIEVNIGDGGGECIYKGEVTGLEATFRGGEKSRLLVRGMNKLHRLLR
                     KRKSVTFMDKNDQQILNQVVSDVSGLSLKWKHDVSITYKHVYQHNLTDLEFIRMRAAR
                     IGAHVWCVDKEVFVQKPDLQSKPIAFLKVGESGDNAVKSFTPRLSSAAIVNKVTVKGW
                     NPEKKELITGNATVQTSKLGNEHSVAGSKDMGNEETFTVDHPIWSPDEATALAKARLQ
                     DLALSYVTGEAEVPGAPVYDLGKVVQIISNPEDSGSDDPFNGNYYIMGVTHRYSVPRN
                     KEGGYTTILRLARDAQKGPKE"
     gene            complement(3203..3850)
                     /locus_tag="VMZ53_25950"
     CDS             complement(3203..3850)
                     /locus_tag="VMZ53_25950"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31981.1"
                     /translation="MSPSSDNKSFSPYTPVAQSPGKEGAAKFWIKSLDEPSLVVSAQF
                     NPKELQIDRQVPWSPPGEAGKENSKNKSGGVDLEFTGAKGRSLSVELLFDGYEEEAEP
                     GFVGKVAESVATLEALASVRKPGEKKEDERRPHWCVCSWGTALQSGGEKKFKCVIESI
                     STKYTMFDNEGTPLRATVTLKLTEADSVSVKKDPAKKGGGAAKKPAGGSPPAGKK"
     gene            complement(3847..>7461)
                     /locus_tag="VMZ53_25955"
     CDS             complement(3847..>7461)
                     /locus_tag="VMZ53_25955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAP7 domain-containing protein"
                     /protein_id="HUS31982.1"
                     /translation="AAQVMASAIAPLMQAQTRTPVGSPQVTAPVMSATLRSMLTTMLE
                     RSAQPAHGELATRSSQYAPAMVTPPAPREEETSAASDLADRYAEQRARIVELQRVARV
                     TAEREAVARTAPAGEQAARIAEIQQRATEAAARREEATRQLDTRRATTQAERVAAAET
                     ERARLEERIAQRLADRARQQDTQRLHETAREAAARDARIAPAVPQAETRADIAPPSVQ
                     SRIAAELANAVSALPPELASMVASSISQRPDRAAQAIADLGEALRTVELLARTTASGG
                     SIAPSRGPRLVMPAGLGGLVSTVEHTTQPIGAAGRVPQFAPTAGAPMFAAPVRDARVP
                     AMTWLAPMQTRATATPAPTSALGAAMQSSPAALHHVAWSDRWLARFAGAQAQSLDVFS
                     TASGESRLAMLAAAAPGAVFVAPSADEPQVAAPSRTDIAAARSSEVRRYDDDAETPDD
                     VLFAIAAAARSSRPSARKSDAAPATPAAPAYDPMVAPTRETLADVVAHAAPGAPGAGL
                     SAQLASSPFAPALRHVLPIGSAPSFDVRALFGSAVGATYLAGLLSSATDELVVPRSLP
                     TWALAALGETPMGAMPAIGERAVESFEPTYVAPADVANLEGEGVAQAAAAAAPLTTLR
                     SALLSFDVETFAATATAPTLASRITAESTSPSLARALVDTLSLPMLADSAAMRDPGTL
                     DVAGGTAHVPSYGAPGMVADRAHAWSVAQERSSADLAYDFVTPELVLAARVYGLGPAE
                     AAQAARLAIAGPGALSAMAGAVDRTFVQAMAIEAERRHGRTQIATAYPLIATADGTQA
                     FDSPSARDAAFARDVAQGVPSAAAPPLFAPSASSFGVEKRAPRGAYLWPAATTAALGL
                     TASMPDGEQSMSVAALELLAAQAVAELGTYSALGEISDVERRAVASVASRAESGATIT
                     TPGSHAEPSDDDILSSAAAFVPAGRRDKFQALYLALSQSPSGRTASPAARAARALALA
                     GRGDEAITARERANVAWDVLPVVFGQRDREPAAGEERTGATVSTGEQASRMLRRREEV
                     RAMDPTYVERPGLGPLSARAGEALGSYVAPAQTAPAAHHRDRDAGAVLRAPSAAHELV
                     QTGRPAGRFGGGEVEIPTWFEQAARKMLADRSAAASTDFSLAELTLVTSAPAPQLAAS
                     SRAPGGSSPSRAPAPAADGAAGGEKKGAEDIEKIANEVYREVLVLMDIARARNGEPYL
                     "
BASE COUNT         1057 a         2572 c         2484 g         1348 t
ORIGIN      
        1 gcgccgccgc tcgcgccacc ttgtgcgcct gcgccggcgc tgccggatgc actgccgctc
       61 gcgctgcccg atgcgccgcc ttgcgcgccc gcgtctgctt ttccgctcgc gccaccggct
      121 gcgccgcctt gtgcgccgcc gctcgcgcct gcgtctgctt ttccgctcgc gccaccggct
      181 gcgccgcctt gtgcaccgcc gctcgcgcca ccttgtgcgc ctgcgccggc gctgccggat
      241 gcactgccgc tcgcgttgcc cgatgcgccg ccttgcgcgc ccgcgtctgc ttttccactc
      301 gcgccgccgc tcgcgctgcc cgatgcgctg ccgctcgcgc tacccgatgc accgccactc
      361 gcgctgcccg atgcgctgcc gctcgcgctg cccgatgcgc cgcccttcgc tccgccgctc
      421 gcaccgccgc tcgcgctgcc cgatgcaccg ccgctcgcgc tgcccgacgc tccaccgctc
      481 gcgctgcccg atgcactgcc gctcgcgccg cccgatgcac cgccgctcgc gctgcccgat
      541 gcactgccgc tcgcgctgcc cgatgctccg ccactcgcgc cgcccgatcc gccgccgccg
      601 ccgccgccac cgccaccacc accaccacca ccgccgctgg cgggtttggc tttcgcctcg
      661 atctgatctt tcgctttctc gacggcagcg ccgccgagct cgcctttgcc ggagcccgac
      721 acgttcgtcg ccggcgagtt cgacacgttg atctgcaccg acgcgccgtt gacggtcacg
      781 ccttgtgctt tcacgccgaa cgtcgcgcct gcatgcgaga gcaccgattg cgcactgccc
      841 ttgatgccct cggtcgtacc gaccttgagc gtcttcgagt gcatgatgat gttgtcggcg
      901 gccttcacct cgatgtcgcc ggcgcactcg atctggacga gcttgcccgg cgaatcgagg
      961 acgatcttgt tcttcttcgt cttgtcgacg atcgtgattt tttcggagcc gtctttgtcg
     1021 tcgaagatga tgcggtgccc cgcgcgggac ttgatcatct tcgtgttgtt cttgccgctc
     1081 tgattgacct cgacgggctc ctgcttcttg ctccagagcg caccgatcat gatcgggcga
     1141 ttgatgtcgc cgtgctcgaa cacgacgagg acttgatcgt ccttctccgg caggaagtac
     1201 gtgccgcgtt ctttgccggc catcgggatc gcgatgcgcg cccagtaggt cgcgtcttga
     1261 tcgttcatcc acgggtactt gaccttgatc tggtacccga tcgtgttgcc gttgccttcg
     1321 gtgttatcga cgacgatcgc gagatgaacg ccacggatct tgccggcgcg aagcgcctcc
     1381 ggcatcacgc ggtcgatcgg gaagaagctc atgcgatgtc gatcgtcgaa gcgtcgtagt
     1441 tgctgttgtc ttgcgacgtg agcttggcgg acgagccgtc catcttgatg tcttggcccg
     1501 ctttgatgtc gatcgtgtct tgcgcgttga tcttcacgtt cttgccggcc gtcatcttga
     1561 ggacgccgtc cttgcacgtg atctcgaggt tgccttccat cgaggagatc gagacgccct
     1621 tgtcaccgct catcggcggt tggtggacgg cgcacacgtt cggtcccgcg ccggcctttg
     1681 cgaagttgcc aacgccgacc atcagcttcg tcgacttgtc ggcgaagtac accttcgtct
     1741 tgtccgagtc gtcgaacacg aagcgatgac caacgcggct gcgaaagccg cggaagttgt
     1801 tcttgccgtc ttcgttgggc tccggcgaga agtcgggcgt gctccagaca ccgccgagga
     1861 tgatcggctg cgcggtgtcg ccaccgataa acgcgacgac cacgacgtca tcgacatcgg
     1921 gcagcgtgta ccagccgaac ttctggcctt ccatcggcgt aacgagctgc gcccagtgcg
     1981 tctgatcggt gtcgccttcg tcgagccacg gcaggcgaat cttcacgcga tcgagattct
     2041 cgggatcctt gttctggcag accacgccgt agtgcaaccc gaggagcagg ttgtccaacg
     2101 aatcgagctt gggtccgcct gcgcgcattt attccttcgg tcccttttgc gcatcgcgcg
     2161 cgagccgcag gatcgtcgtg tagccaccct ctttgttgcg cggcaccgag taacggtgcg
     2221 tgacgcccat gatgtagtag ttgccgttga acggatcgtc cgagccgctg tcctcggggt
     2281 tcgagatgat ctggacgacc ttgccgagat catagacagg agcgcccggc acttcggctt
     2341 cgcccgtgac gtacgagagc gcgagatcct gcaggcgcgc tttcgcgaga gcggtcgctt
     2401 cgtcggggct ccagatcgga tgatcgaccg tgaacgtctc ctcgttgccc atgtccttcg
     2461 agcccgcgac cgagtgctcg ttaccgagct tcgatgtctg caccgtggcg ttgccggtga
     2521 tgagctcttt cttctcgggg ttccagccct tcaccgtcac cttgttgacg atggccgccg
     2581 acgacaggcg cggcgtgaag ctcttgaccg cgttgtcacc cgactcgccg accttgagga
     2641 acgcgatcgg cttgctctgt agatcgggct tctgaacgaa gacctctttg tcgacgcacc
     2701 agacgtgcgc gccgattcgc gctgcacgca tgcggatgaa ctcgaggtcc gtcaggttgt
     2761 gctggtagac gtgcttgtac gtgatgctga cgtcgtgctt ccacttcagg ctcagaccgc
     2821 tgacatcgct gacgacctga ttgaggatct gctgatcgtt cttgtccatg aacgtgactg
     2881 acttccgctt gcgcaggagc cggtgcagct tgttcatgcc gcgcacgagg agacgcgatt
     2941 tctcgccgcc tcggaacgtc gcttcgaggc ccgtgacctc gcctttgtag atgcactcgc
     3001 cgccgccgtc gccgatgttc acttcgatcg ggccggccat ctcgatcttg ggcgagtagt
     3061 tgttctcttg gttcgagaga acgatcgcgc acatgtccgg ctggaacatg tcgcgctcga
     3121 tcgaaaagcc ctgcacgtcg gcactctcga tctcgagctt gccgatcttg atcttgaagt
     3181 tggtttgcgc gcccacggcg gcttacttct tcccggccgg agggctgcca cctgcaggct
     3241 tcttcgcggc tcccccaccc ttcttggccg ggtccttttt cacgctcacc gagtcggcct
     3301 cggtgagctt caacgtgacc gtcgcgcgaa gcggcgtgcc ttcgttatcg aacatcgtgt
     3361 acttcgtcga gatcgactcg atgacgcact tgaacttctt ctcgccgccc gactgcagag
     3421 ctgttcccca cgagcacacg caccagtgcg ggcggcgctc gtcttccttc ttctcgccag
     3481 gcttgcgcac cgacgcgagt gcttcgagcg tcgcgaccga ctcggcgacc ttgccgacaa
     3541 atccgggctc ggcttcttcc tcgtagccgt cgaacaggag ctcgaccgag agcgaacggc
     3601 ctttggcgcc ggtgaactcg aggtccacgc cgcccgactt gttcttgctg ttctctttgc
     3661 cggcttcgcc tggagggctc cacggaacct gccgatcgat ctgcagctcc tttggattga
     3721 actgagcgct gacgacgagg ctcggctcgt cgagactctt gatccagaac ttcgcggcgc
     3781 cctccttgcc cggtgattgt gcgaccgggg tatagggcga gaagctcttg ttgtcgctgc
     3841 ttgggctcat aggtagggct ctccgttacg cgcacgggcg atgtccatga gaacgagcac
     3901 ctcgcggtag acctcgttcg cgatcttttc gatgtcctcg gcgccctttt tctcgccgcc
     3961 agccgcgcca tcggcggcag gagccggggc gcgcgaaggc gagctgccac cgggtgcgcg
     4021 agacgacgca gcgagctgcg gcgcaggagc cgacgtgacg agcgtgagct cggcgagcga
     4081 gaagtccgtc gacgcagctg cggagcgatc cgcgagcatc ttgcgggccg cttgctcgaa
     4141 ccacgtcggg atttcgactt caccgccgcc gaaccgaccg gccgggcgac ccgtttgcac
     4201 gagctcgtgc gcagccgacg gagcgcggag gacagcgccc gcatcgcgat cgcggtgatg
     4261 cgcagcgggt gcggtctgcg caggcgcgac gtacgagccg agcgcttcgc cggcgcgcgc
     4321 cgagaggggt ccgagacccg gacgctcgac gtacgtcggg tccatcgcgc gaacctcttc
     4381 gcgacggcgc agcatgcgag acgcttgttc gcccgtcgag accgtagccc ccgtgcgctc
     4441 ttctcctgcc gccggctcgc gatcgcgctg accgaagacg acgggcaaca catcccaggc
     4501 gacgttcgcg cgttcgcgcg cggtgatggc ttcgtcgccg cgaccggcca gtgcgagcgc
     4561 gcgtgcggcg cgtgcagcgg gcgatgcggt acggccagat ggcgattggc tcagagcgag
     4621 gtagagcgcc tggaacttgt cgcgacgtcc ggctggaacg aacgcagccg cgctcgacag
     4681 gatgtcgtca tcacttggct cggcgtgcga accgggcgtc gtgatcgtcg cgcctgattc
     4741 cgcgcgcgat gcaaccgacg cgacggcgcg acgttcgaca tcgctaatct caccgagcgc
     4801 cgagtacgtg ccgagctcgg caacagcctg cgcggcgagc agctcgaggg cagccaccga
     4861 catcgactgc tcgccgtcgg gcatcgaggc cgtgaggccg agtgcagcgg tcgttgcagc
     4921 gggccagaga tacgcgccac gcggcgcgcg cttctcgacg ccgaacgacg acgcgcttgg
     4981 tgcgaacagc ggcggcgccg cagccgaggg cacgccctgc gctacgtcgc gcgcgaaggc
     5041 ggcgtcgcga gcggacggcg agtcgaacgc ctgcgtacca tcggcggtcg cgatcagcgg
     5101 atacgcggtc gcgatctgcg tacgaccgtg acgacgctca gcttcgatcg ccatcgcttg
     5161 cacgaacgta cgatcgaccg cgccagccat cgccgacagt gcacccgggc ctgcaatcgc
     5221 gagacgtgcc gcctgcgctg cttctgcggg accgaggccg tagaccctcg ccgcgagcac
     5281 gagctcgggc gtgacgaaat cgtatgcgag gtccgcgctc gagcgctcct gcgccactga
     5341 ccacgcgtgc gcgcgatccg cgaccatgcc cggcgcgccg tacgacggaa catgcgcggt
     5401 gccgccggcg acgtcgagcg tgcccgggtc gcgcatggcg gcgctgtccg cgagcatcgg
     5461 cagcgagagc gtatcgacga gcgcacgcgc gagcgacgga gacgtcgact ccgccgtaat
     5521 gcgcgatgcg agcgtcggcg ccgtcgccgt agcggcgaac gtctcgacgt cgaacgacag
     5581 cagtgcgctg cgcagcgtcg tgagcggtgc tgctgcagca gcggcctgcg cgacaccttc
     5641 gccctcgaga ttcgcgacgt cagccggcgc gacatacgtc ggctcgaacg actcgacggc
     5701 gcgctcgccg attgcgggca tcgctcccat cggcgtctcg ccgagcgcgg cgagtgccca
     5761 cgtcggaagc gagcgcggaa cgaccagctc gtccgtcgcc gacgacagca gaccggcgag
     5821 ataggtggcg ccgaccgcgg aaccgaacag tgcgcgaacg tcgaagctcg gtgccgagcc
     5881 gatcggcagg acgtgacgga gcgccggcgc aaacggcgac gacgcgagct gcgcagagag
     5941 gccagcaccc ggtgcgcctg gcgctgcgtg cgcgacgaca tcggcgagag tctcgcgcgt
     6001 cggcgcgacc atcgggtcat acgcaggcgc ggccggcgtt gcgggtgctg catccgactt
     6061 gcgagcagat ggacgcgacg agcgcgcggc cgcagcgatc gcgaagagca cgtcatcggg
     6121 cgtctcggcg tcgtcgtcgt agcgacggac ttcgctgctg cgagccgctg cgatatcggt
     6181 gcgcgagggc gccgcgacct gcggctcgtc ggccgacggc gcgacgaaca ccgcaccggg
     6241 cgctgccgcc gcgagcatcg cgagacgcga ttcgccggag gccgtgctga acacgtcgag
     6301 cgactgcgct tgtgcacctg cgaagcgagc gagccagcga tcgctccacg cgacgtgatg
     6361 aagcgctgcg ggcgacgact gcatcgcggc accgagcgcg ctcgtcggag cgggcgtcgc
     6421 ggtagcacgc gtctgcatcg gcgcgagcca cgtcatcgcg ggaacgcgcg cgtcgcgcac
     6481 gggcgcagcg aacatgggag cgcccgcggt gggagcaaac tgcggaacgc gaccggcggc
     6541 gccgatcggc tgcgtcgtgt gctcgacggt cgaaacgagg ccgccgagac cggcgggcat
     6601 cacgaggcgc gggccacgag acggagcgat cgagccgccg cttgcggtcg tgcgagcgag
     6661 aagctcgacg gtgcggagtg cttcgccgag atcggcgatc gcctgcgctg cgcgatcggg
     6721 acgctggctg atcgagctgg cgaccatcga cgcgagctcg ggcggaagtg ccgagacagc
     6781 gttggcgagc tcggccgcaa tgcgcgactg cacgctcggc ggcgcgatgt cggcgcgggt
     6841 ctcggcctgc ggcaccgccg gcgcgatacg cgcatcgcga gccgctgctt cacgtgcggt
     6901 ctcgtgcagg cgttgcgtat cttgctggcg ggcgcggtcg gcgagtcgct gcgcgatgcg
     6961 ctcctcgaga cgagcacgct cggtttcggc agctgcaacg cgctctgctt gcgtcgtcgc
     7021 gcgacgcgtg tcgagctggc gcgtagcttc ttcgcgacga gccgccgcct cggtggcgcg
     7081 ctgctggatc tcggcgatgc gtgcagcctg ctcgcccgca ggcgccgtgc gagcaactgc
     7141 ttcgcgctcg gccgtcacgc gtgcgacgcg ctggagctcg acgatgcgcg cgcgttgttc
     7201 ggcatagcgg tccgccaggt cgctcgcggc ggaggtctcc tcttcgcgcg gcgctggtgg
     7261 cgtcaccatc gcaggtgcgt actgcgacga ccgcgtcgcg agctcgccgt gcgccggctg
     7321 cgccgagcgc tcgagcatcg tcgtcagcat cgagcgcagc gtcgcgctca tcaccggcgc
     7381 cgtgacctgc ggcgagccga cgggcgtgcg cgtttgcgcc tgcatcagcg gcgcgatcgc
     7441 cgacgccatc acctgcgcgg c
//
LOCUS       DATLBZ010000515         8305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1412364, whole genome shotgun sequence.
ACCESSION   DATLBZ010000515 DATLBZ010000000
VERSION     DATLBZ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8305
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412364"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..731)
                     /locus_tag="VMZ53_25960"
     CDS             complement(360..731)
                     /locus_tag="VMZ53_25960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31983.1"
                     /translation="MKLSIACVAALSLFGCADEVSTSGDTEVSVVGVKEAQNREARLS
                     EVRLRIEDVRFSEKVGTWAESLIPDSMDKACVNCELVFVHQSDGRIDNTEIWTADGNF
                     LCRVKQTSIALLQNTCSKFQQ"
     gene            complement(785..1180)
                     /locus_tag="VMZ53_25965"
     CDS             complement(785..1180)
                     /locus_tag="VMZ53_25965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31984.1"
                     /translation="MKLFLAGVAAVAMFGCADQPETTGRGGISPAKHFANPAVSGATQ
                     FEGMQTAWHMSLADLSFSDQIGTVSESMNPQGNGNPDEGCADCEVIAIRTGDGLLENI
                     DLLTVDGTYLCSIRQTAVAVLSNSCGWIR"
     gene            complement(1421..3598)
                     /locus_tag="VMZ53_25970"
     CDS             complement(1421..3598)
                     /locus_tag="VMZ53_25970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31985.1"
                     /translation="MHRLIVVIAFLVAARGTAHADLSSQLYGDVRDVIEELIQTEVTT
                     SVVTTVKVRSPALEFYMHGTLERLGSPYWGSLGRVLKDDVTVATADFVYWHVSTGGGS
                     GDVIESAKKFFDCARKNRGDDPACKRLLEAIVTQHRPLLEVECRREKPSPDRRIACDL
                     GLATLAALKGRGEARHHLVDAMSDIVLLEVGGSDLGERMRDVLTKWLDLPKDLPTPLL
                     EALANPDLGAELSDEALEKRCKDWKEMQKALDDPSSPYAWLCFAITHEQLVPQLQASV
                     VVRENGKSFSQKIDHWVIAAALKDFDSDRADDDLVYRMFADLAFDAQCPAAKDGGDAP
                     PEAAAWPCGGKRLEPGAQVSVYWLRRPPWMLTLGQNGKFTANNLPQKFLIASVEKFRR
                     LYRRINELRQVVPPSLEKYLFHAGKTVQPDTKKALRALHRMGRLVAELRGRWYLWSQT
                     QNTKAFAELDVAELLEVARNSLDQATIEKNQALAFLDKQASGGAATVDIGDWLRMVMR
                     ADYRSLAMESLRSALDLRLADKSRPKETFFLSLTAYLLDNSEGVGEQVARSAFRASAK
                     ELLLSMSHRGVPRADDRLRFRITPRLMAGLSFADSYADTDGDSRRRVVAAYWPTAMVA
                     VNDYVGLEFSGLDLIGPLTEMALRPAGTYDQQQKVALDFLRPRAGLWVAMPQFSRRVA
                     LTVGGGGRFLELKRLDDGATPMVRAQYSYKPSLMFDAGIQFVF"
     gene            complement(3696..5795)
                     /gene="uvrC"
                     /locus_tag="VMZ53_25975"
     CDS             complement(3696..5795)
                     /gene="uvrC"
                     /locus_tag="VMZ53_25975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HUS31986.1"
                     /translation="MIAADVLDRIPTEPGVYLFKDAKGQVVYVGKAQNLRTRVRQYFR
                     VGGDERFFVAAGFLGRAVADLETIVVSSSKEALLLENHLIKKHQPRFNVKLRDDKQYL
                     VLRLVDPRLPASAGGPKTPAPPPDGEEPGKIQKRELFPRVEVVRNIRDDNASYFGPYH
                     SATSARETLRTLNRHFQLRTCTDHVLETRGRPCLQYQIKRCSGPCAIDVPPAAYAEQV
                     EDVKMFLGGKSAELVSRLRTRMEKRADREDFEVAAVLRDSIAAVERTLAKQHIVQDDF
                     VDQDVWGLYREADNVEVAVLFIRGGKLVGRRVFCQKDQELPDATVIAEHVQQYYATGT
                     YIPDEIVVGVVLEDGAVLADWLGAARGKKVKLVEPRRGIRARLVELADRNAAASAQSR
                     RNKDADADALLQKVADRLGLTRPPKRIECFDIAHIQGTDTVASMVTFVDGVPARGLYR
                     KFKIRTVDNNDFASMYEVLTRRFKRTVGDDPAWAKPDLLVIDGGKGQLGMAVTALTDL
                     GVKLGGEDGVEVIGLAKERELEAGSAPDRIYRRNVKDSIALRANSPELYVLARIRDEA
                     HRFANTFHRDRRTKASLRSQLDDIPGIGTTRRQRLLKHFGSVRAVRQATLDELAKAPG
                     MNRKAAEQVAKFFAELEPEPAGTGDLDANVEGQTVEVVPEPVYEGDDSDGATATAVSD
                     DTTPTDVDDSDGAPQSS"
     gene            complement(5831..6928)
                     /locus_tag="VMZ53_25980"
     CDS             complement(5831..6928)
                     /locus_tag="VMZ53_25980"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HUS31987.1"
                     /translation="MPWFLALMSLAFLIIVHEGGHFFVARWCKMRVDRFSIGFGPGIL
                     KKKTKSGTVFQLAPIPFGGFVEIRGMNIAEDVDPDDKLAYPNRPAWQRFLTIFAGPAT
                     NYLSAVLIVFMLYNCHGQESSWKFYGVAAVKEDYDAFGKLKVGDRILAVDGDPILAAG
                     EYVGPDGTRLHSDDLGLVGRVSAKKGAPVTLTILRDGHKQEVLVQPKLVFRDIPYDME
                     PKYLLGIAPSNSSDTIDIGVVASARESLIYPVVLTQRIGTFLYEVVRGRQEADPGGPK
                     RIVDEFKSAWELGWVTGLQLLAGLSVYLGLFNLLPLPALDGGRLVFLTYELVTRRRAN
                     PKIEATVHMAGIMVLAVVMILVTLRDFKVFS"
     gene            complement(6992..7540)
                     /locus_tag="VMZ53_25985"
     CDS             complement(6992..7540)
                     /locus_tag="VMZ53_25985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31988.1"
                     /translation="MVLPFGDDEEYVGIAVRRIAEYLRALELSFEILAIDEDSGDNSH
                     AVLALLRAEVPELRVTHAPGRGRGVDVGASRAQGTLIAVATPDVAAGSLDGVGDACRR
                     LLAGEGDAEVALTRYTVAHRVRGLVAFRGARLVGAAMHRRLAKRLQVRHLAVRVAGAT
                     GITAKSAGSLFRAFAARGNRVA"
     gene            complement(7745..>8305)
                     /locus_tag="VMZ53_25990"
     CDS             complement(7745..>8305)
                     /locus_tag="VMZ53_25990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUS31989.1"
                     /translation="LVPIKGVDVAIDAMTHVGTNIELVIAGDGPERAALESRVARIAP
                     SSSRAHVARDRAAPGQRDPVERVHFVGQLDATARDELLGRASLVVVPSRVLPNGRSEG
                     TPLVALEALAAGVPVIASDVGGLRELDAIVRVPPDDPRALAAAIDRVLASPPDGDELR
                     ASISHLSWPRVAQRLLDFTLDRLVRA"
BASE COUNT         1525 a         2725 c         2663 g         1392 t
ORIGIN      
        1 caggtagccg cacatcccgg cgaggccgta gaacattccg gcggtcgagc gcccgaacgg
       61 gcccatgacc atcgtgtggg cctgaagcga gatcatcgcg atgagcagga acagtcccgc
      121 tcccagcccg atgaccccgg ctacctcgcg gcgccggtcg tcaccgcctc caccaccccg
      181 ccgaccctcg cccgaagaac tgctgttaat ctttcggttt ttggacatgg ctgtcgctcg
      241 gctccctgga gcactttatc ctacatggcc gcacaccagg gacccacttt tttccctcgg
      301 cagagccagc ggagcgacaa cccgaaatga actgctaacg aaccgcgaac gatcgaagac
      361 tactgctgga actttgagca ggtgttctgc agcagcgcga tcgaagtctg cttcacgcgg
      421 cagaggaagt tgccgtccgc cgtccagatc tccgtgttgt cgatgcggcc atcgctctgg
      481 tgaacgaaga cgagctcgca gttcacgcac gccttgtcca tcgaatcagg aatgagcgac
      541 tcggcccacg tgccgacctt ctcggagaag cgaacatcct cgatgcgaag gcggacctcg
      601 ctcaggcgcg cctcgcggtt ttgcgcttcc ttgacgccaa cgacgctgac ttcggtgtca
      661 ccgctcgtcg agacttcgtc cgcacaacca aacagcgaga gagcagcgac gcaagcgata
      721 gagagcttca cgagttcctc cagagttatc ggtaaaagtg ccgtagcaaa aatgggatga
      781 gtgctcagcg aatccagccg cagctgttcg agagcacggc gaccgccgtt tggcggatgg
      841 aacagagata ggtgccgtcg accgtcagca ggtcgatgtt ctcgagcaag ccatcgccgg
      901 ttcggatcgc gatgacttcg cagtcggcac aaccctcgtc ggggttgccg ttgccctgcg
      961 ggttcatcga ctcggagacc gtgccgatct gatcgctgaa cgagaggtca gcgaggctca
     1021 tgtgccaggc cgtctgcatg ccctcgaact gggtggcgcc agagacggcc gggttcgcga
     1081 agtgcttcgc ggggctgatg ccccctcgac ccgtcgtttc gggctgatca gcgcaaccga
     1141 acatggcgac agcggcaaca cctgcgagga agagcttcaa gaccggcctc cgttgagtag
     1201 tgagaaccaa catcagcaca cgtgacagac cattatagtc ggcattgcat tccaaaaatc
     1261 cagatatgga attcggaaaa ataaaaaatc gtgcgcgaca tcgtgcgacg tcgacggtgt
     1321 cgcaacgcgc gcgtgtagga cacgcggcgc acgagacaca ctgattagtt gatttccgct
     1381 gtgtgggata acagcgctac ggcacgcgct ggcgagaacg tcagaagacg aactggatgc
     1441 cagcgtcgaa catcagcgac ggcttgtacg aatactgagc gcgcaccatc ggcgtggcgc
     1501 cgtcgtcgag tcgcttcagc tcgaggaagc gtcctccgcc accgactgtc agcgcgactc
     1561 tgcgagagaa ctgcggcatc gcgacccaca ggcccgcgcg cggacgcaag aagtcgagcg
     1621 cgaccttctg ctgctggtcg tacgtgccgg ctggacgcag tgccatctct gtcagcgggc
     1681 cgatgagatc gagccccgag aattcgagac cgacgtagtc gttcacggcc accatcgcgg
     1741 tgggccagta cgcagcgaca acgcgacgcc gcgagtcgcc atcggtgtcg gcgtagctgt
     1801 cggcgaacga gagccccgcc atcagccgag gcgtgatgcg aaagcgcagc cgatcatccg
     1861 cgcgcggcac tccgcgatgc gacatcgaga gcaagagctc cttcgccgat gcgcggaacg
     1921 ccgagcgcgc gacttgctcg ccgacgcctt cgctgttgtc gagcaggtat gccgtgagcg
     1981 agaggaagaa cgtctccttt ggccggctct tgtccgcaag cctcagatcg agcgccgagc
     2041 gcagcgattc catggcgagc gagcggtaat cggcacgcat caccatgcgc agccaatcgc
     2101 cgatatcaac ggtcgctgcg ccgccgcttg cttgcttgtc gaggaatgcg agcgcttggt
     2161 tcttctcgat cgttgcttga tcgagagagt tgcgcgcgac ctcgagcagc tctgcaacgt
     2221 cgagctcggc gaacgccttc gtgttctgcg tctgcgacca cagataccag cgcccgcgca
     2281 gctcggcgac gagccgcccc atccgatgca gcgcgcgcag cgccttcttc gtatcgggct
     2341 gcacggtctt gccggcgtga aacaggtact tctcgagcga aggcggcacg acctgcctca
     2401 gctcgttgat gcggcgatac agccggcgga acttctcgac ggacgcgatc aaaaatttct
     2461 gcggcaggtt gttcgcggtg aacttgccgt tctggccgag cgtcaacatc catggcgggc
     2521 gccggagcca atacacgctg acctgcgcgc cgggctcgag gcgcttaccg ccgcacggcc
     2581 acgccgctgc ctcgggagga gcgtcgccgc cgtccttcgc agctggacac tgcgcatcga
     2641 acgcgaggtc cgcgaacatt cgatagacga gatcgtcgtc ggcgcgatcg ctgtcgaagt
     2701 ctttgagcgc cgcggcgatg acccagtgat cgatcttctg gctgaacgac ttgccgttct
     2761 cgcgcaccac caccgacgcc tgcagctgcg gaacgagctg ctcgtgcgtg atcgcgaagc
     2821 agagccacgc atacggcgac gacggatcgt cgagcgcctt ctgcatctcc ttccagtcct
     2881 tgcagcgctt ctcgagagct tcatcggaaa gctccgcgcc gagatcggga ttcgcgagcg
     2941 cctcgagcag cggcgtcggg agatccttcg gcaggtcgag ccacttcgtg aggacgtcgc
     3001 gcatgcgctc gccgagatcg gagccgccga cttcgagcag cacgatgtcg ctcatcgcat
     3061 cgacgaggtg atggcgcgct tcgccgcggc ccttcagcgc agcgagtgtc gcgaggccga
     3121 ggtcacacgc gatgcggcga tcgggcgatg gcttctcgcg ccgacattcg acttcgagca
     3181 atggccgatg ctgcgtgacg atcgcctcga gcaagcgctt gcacgcgggg tcatcgcccc
     3241 gattcttgcg cgcgcagtcg aagaacttct tcgcgctctc gatcacgtcg ccactgccac
     3301 cgcccgtcga gacatgccag tagacgaagt ccgccgtcgc gacggtcacg tcgtccttga
     3361 gcacgcgacc gaggcttccc cagtatggcg agccgagtcg ctcgagcgtg ccgtgcatgt
     3421 agaactcaag cgcgggcgag cgaaccttca ccgtcgtgac caccgaggtc gtcacttcgg
     3481 tctggatgag ctcttcgatc acgtcgcgaa cgtcgccgta gagctgcgac gacagatcag
     3541 cgtgcgccgt gcctcgcgcc gccaccaaga acgctatcac cacgatgaga cgatgcatgg
     3601 agcgagcatc gtaacggcga atgcgccgcg atgaccgcga ggtgcgtcag attcgtagcg
     3661 tcacgatgac cgcgaggtgc gccagattcg ttgcgtcacg acgactgcgg cgcgccgtct
     3721 gagtcgtcga catcggtcgg cgtcgtgtcg tcggagaccg ctgtggccgt cgcgccgtcg
     3781 gagtcgtcgc cttcgtagac gggctcgggc acgacctcga cggtctgacc ttcgacattc
     3841 gcgtcgaggt cgccggtgcc cgcgggctcg ggctcgagct cggcgaagaa cttcgcgacc
     3901 tgctcggcgg ccttgcgatt catgcccggc gctttcgcga gctcgtcgag cgtcgcctgc
     3961 cgcaccgcgc gcacactgcc gaagtgcttg agcagccgct ggcgccgggt cgtgccgatg
     4021 cccggaatat cgtcgagctg cgagcgcagg ctcgccttcg tgcgccgatc gcgatggaac
     4081 gtgttcgcaa accggtgcgc ctcgtcgcga atgcgcgcga gcacgtacag ctcgggcgag
     4141 tttgcgcgca gcgcgatcga gtccttcacg ttgcggcgat agatccgatc cggcgcgctg
     4201 cccgcttcga gctcgcgctc cttcgcgagg ccgattacct cgacgccgtc ctcgccgccg
     4261 agcttcacgc cgagatcggt cagcgcggtg actgccatgc cgagctggcc cttgccgccg
     4321 tcgatgacga gcaagtcggg cttcgcccag gcggggtcat ctccgacggt gcgcttgaac
     4381 cggcgcgtga gcacttcgta catcgacgcg aagtcgttgt tgtcgaccgt gcgaatcttg
     4441 aacttgcgat agaggccgcg cgctggaacg ccgtcgacga acgtgaccat cgatgcgacg
     4501 gtgtcggtgc cctgaatgtg cgcgatgtcg aagcactcga tgcgcttcgg aggccgcgtg
     4561 aggccgagcc gatcggccac tttctgaagc agcgcgtcgg cgtctgcatc cttgttgcgc
     4621 cgcgattgcg ccgacgccgc agcgttgcga tccgcgagct cgaccaagcg cgcgcgaatt
     4681 ccgcggcgag gctcgacgag cttcaccttc ttgccgcgcg cggcgccgag ccagtccgcg
     4741 agcaccgcgc cgtcttcgag cacgacgccg acgacgatct cgtcggggat gtacgtgccc
     4801 gtcgcgtagt actgctgcac gtgctcggcg atcaccgtcg cgtcgggcag ctcttggtcc
     4861 ttctggcaga acacgcgccg cccgacgagc ttgccgccgc gaatgaacag cacggcgacc
     4921 tcgacgttgt ccgcctcgcg atagaggccc cacacatctt gatcgacgaa gtcgtcctgc
     4981 acgatgtgct gcttcgcgag cgtgcgctcg accgctgcga tcgaatcgcg cagcacggcc
     5041 gccacctcga agtcttcgcg atcggcgcgc ttctccatgc gcgtgcgcaa gcgcgagacg
     5101 agctcggcgc tcttgccacc gaggaacatc ttcacgtcct cgacttgctc cgcatatgcc
     5161 gccggcggca cgtcgatcgc gcatggcccg ctgcagcgct tgatctgata ctgcaggcac
     5221 ggtctgccgc gcgtctcgag cacgtgatcc gtgcacgtgc gcagctggaa gtgacgattg
     5281 agcgtgcgca gcgtttcgcg cgcgctggtc gccgagtgat acgggccgaa gtagctcgcg
     5341 ttgtcgtcgc ggatgttgcg aacgacctcg acgcgcggga acagctcgcg cttttggatc
     5401 ttgccgggct cctcgccgtc gggcggtggc gcgggcgtct tcggcccgcc agcggaagct
     5461 ggcaagcgag gatcgacgag ccgcagcacg aggtactgct tgtcgtcgcg cagcttcacg
     5521 ttgaagcgcg gctgatgctt cttgatgagg tggttctcga gcagcagcgc ttccttcgac
     5581 gacgacacga cgatcgtctc gaggtccgcc accgcgcgtc cgaggaagcc cgccgcgacg
     5641 aagaaccgct cgtcaccgcc tacgcgaaag tactgccgca cgcgcgtgcg gaggttctgc
     5701 gccttgccga cgtagacgac ctggcctttg gcgtccttga atagatagac gcccggctcg
     5761 gtgggaatcc gatcgaggac gtccgcggca atcaccggcg cactttagcg cgccatctga
     5821 cagaggcccg tcagctaaag actttgaaat cccgcagcgt cacgagaatc atcacgaccg
     5881 ccaggaccat gatgccggcc atgtgaacgg tagcttcgat cttgggattc gcgcggcgac
     5941 gcgtgacgag ctcgtacgtc aggaacacga ggcggccgcc gtcgagcgca ggcagcggca
     6001 gcaagttgaa gagtccgagg tagacggaca atccagcgag gagctgcagg ccggtcaccc
     6061 agccgagctc ccaggcgctc ttgaactcat cgacgatgcg cttggggccg cccgggtccg
     6121 cttcctggcg cccgcgcacg acctcataga ggaacgtgcc gatgcgctgc gtgagcacga
     6181 cgggatagat cagtgattcg cgcgccgacg ctacgacacc gatgtcgatc gtgtccgacg
     6241 agttcgacgg cgcgatgccg agcagatact tcggctccat gtcgtacggg atgtcccgga
     6301 acacgagctt gggttggacg agcacttcct gcttgtgacc atcgcgcagg atcgtgagcg
     6361 tgaccggcgc acccttcttc gcagacacgc ggccgaccaa gccgagatca tccgagtgga
     6421 ggcgcgtgcc atccgggccg acgtactcgc ccgccgcaag aatgggatcg ccatcgaccg
     6481 cgaggatgcg atcgccgacc ttgagcttgc cgaacgcgtc gtagtcttcc ttgacggcgg
     6541 cgacgccgta gaacttccac gagctctctt ggccgtggca gttgtagagc atgaagacga
     6601 tgagcaccgc cgacagatag ttcgtcgcgg ggcccgcgaa gatcgtcagg aagcgctgcc
     6661 acgccggacg attcggatac gcgagcttgt cgtcgggatc gacatcctcg gcgatgttca
     6721 tgccgcgaat ctcgacgaag ccgccgaacg gaatcggggc gagctggaac accgtccccg
     6781 acttcgtctt cttcttcaga atgccgggac caaagccgat gctgaagcga tcgactcgca
     6841 tcttgcacca gcgcgccacg aagaaatgac ctccctcatg cacgatgatg aggaacgcca
     6901 acgacatcag agcgaggaac cagggcaccc actgcgagca tagcagcgac gcccgaggga
     6961 ggcccggcct agtcgccgtt actgccgcaa attacgcgac gcgattgccg cgagcggcaa
     7021 aggcgcggaa cagcgagcca gcagacttcg cagtgatgcc cgtcgccccg gcaacgcgga
     7081 cggcaaggtg gcgaacttgg agccgcttcg ctagccggcg atgcatcgcc gcaccgacga
     7141 ggcgcgcgcc gcgaaacgcg accaagccgc gcacgcgatg agcaaccgtg taacgagtca
     7201 gcgcgacttc ggcatcgccc tcaccggcga gcagccggcg acacgcgtcg ccgacaccgt
     7261 cgagcgaacc agccgcgaca tccggcgtcg cgacggcaat gagcgttccc tgcgcgcgcg
     7321 acgcgccgac atccacaccg cgaccacgac caggcgcgtg cgtgacgcga agctcgggca
     7381 cttccgcgcg aagcagtgcg agcactgcgt ggctgttgtc gccggagtcc tcgtcgatcg
     7441 cgagaatctc gaacgagagc tcgagcgcgc ggagatactc ggcgatgcga cgcacagcga
     7501 tgccgacgta ctcctcgtca tcgccgaatg gcaaaaccac cgagacctgc agcctagggc
     7561 tgtcggacat cggcagcggg attagcacca aacgtgccgc cacgagatgc atattcataa
     7621 cgcatcagcc gccagatcgt cacgtgattg ccgcacgtta gccacacaca ggatggcatt
     7681 ggtcggaccg cgatgcgaac gctcttacgc agtgcggcgg ccgctcgtat cgccagcttc
     7741 gcagctacgc acgcacgagg cgatcgagcg tgaagtcgag cagtcgctgc gccacgcgag
     7801 gccacgacag atgcgagatg ctcgctcgca gctcgtcgcc atctggcggt gatgcgagca
     7861 cgcgatcgat cgcagcagca agcgcgcgtg gatcgtcagg cggcacgcga acgatggcgt
     7921 cgagctcgcg cagcccgccg acgtccgacg cgatcaccgg cacgccagca gcgagtgctt
     7981 cgagtgcgac gagtggtgtg ccctcgctgc gaccgttcgg gagcacgcgc gatggaacca
     8041 cgacgaggct cgcgcgtccg aggagctcgt cgcgcgcggt tgcgtcgagt tgcccgacaa
     8101 agtgcacgcg ctcgacggga tcgcgctggc caggcgcggc gcggtctcgt gcgacatggg
     8161 cgcgcgacga acttggcgcg atccgcgcga cacgtgattc gagtgcggca cgctcgggtc
     8221 catcgccggc gatcacgagc tcgatattcg tacccacgtg ggtcatcgca tcgatcgcca
     8281 catcgacgcc cttgatcggc acgag
//
LOCUS       DATLBZ010000516         2884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2907483, whole genome shotgun sequence.
ACCESSION   DATLBZ010000516 DATLBZ010000000
VERSION     DATLBZ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2907483"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VMZ53_25995"
     CDS             complement(<1..1002)
                     /locus_tag="VMZ53_25995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS31990.1"
                     /translation="MCLPSLDETALAQTLASVDPRATIRPPSLRDTVVHDGVARGVDE
                     KLDPLATRLQLGATIGQGGMAIVRKATQTALHREVAVKTLRSDATDEAAPRLLREAWI
                     TGALEHPNIVPVHDLGSDDRGRPLLVLKRIEGEPWQPKSRDLEDNLATLMQVCHAIAF
                     AHNRGIVHRDLKPDNIMIGRFGEVYVLDWGIAVATKDDGSGRLPLAAESREVAGTLAY
                     MAPEMLGGAAVTEQTDVFLLGAILYEIIEGKPPHLLESIGASITSIALCEPSLSPDVP
                     AELVAICRRAMAREAAQRYQTADELRLAVAAFLEHRTSAQIAHESHEKLRELESQLAS
                     "
     gene            1031..1654
                     /locus_tag="VMZ53_26000"
     CDS             1031..1654
                     /locus_tag="VMZ53_26000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31991.1"
                     /translation="MRGLLVTMLIVACAAPRGPAPAPPPLRPTPVAPDASAATPVAEC
                     PARSLGPTPEVVVVQTLAAAQPHLAASTPAANENLMQLAAEHGAAFAVRVMRGAVHGS
                     DSIIYDIAGLFITDKRVVIAKRVATDISVIPGPDGTPTPLQSTVEKLSPAIGHIQLVS
                     GHDVVDHFIDLEHGVHLLALTSSSPRVWKLDGDSGPISGDCQYWWDD"
     gene            complement(1651..>2884)
                     /locus_tag="VMZ53_26005"
     CDS             complement(1651..>2884)
                     /locus_tag="VMZ53_26005"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HUS31992.1"
                     /translation="AVCADAVEPWNAKVNRPLVPRLSSVASWTKKQTTLDPTVRVALE
                     GFVKRWKKRADDCVLDWAELMTDPAALEAGFAGSDVSKKDIERLVAWVKRQLAKPQAP
                     PVDEEGNPILDSEGKAVGPDDDDPAGRFDDEDDPLLLRLTQLKRGGLMPSGGDEMFYE
                     HVAVDEAQDRSALEIKVLVEAVRAPHDDPSQRSVTIAGDTAQRLVFDNNFSGWSDLVT
                     LVAGKSEAAIVRPLKLSYRSTAEVMLLAREILGPELAPEEPLAARPGEPVELHEFGDL
                     GEAVAFLGDALRNLMNREPTASCAVITRHAETADAYFDGLRRAEVPALRRIRRDEFNF
                     QPGVDVTDVAQVKGLEFDYVVMVDVNDASYPAVNWARHLLHIGVTRAAHQLWLVSTGE
                     PSPLIPTALRDGGRMTVG"
BASE COUNT          439 a          985 c          967 g          493 t
ORIGIN      
        1 cgacgcgagc tggctctcga gctcgcggag cttctcgtgc gattcgtgcg cgatctgggc
       61 gctcgttcga tgctcgagaa acgcggcgac tgcgagccgt agctcgtcgg cggtctgata
      121 tcgctgcgcc gcctcgcgag ccatcgcccg tcgacaaatc gcgacgagct ccgccggaac
      181 atccggcgac agcgacggct cgcagagagc gatgctcgtg atcgatgcgc cgatcgattc
      241 gagcaagtgc ggcggcttgc cctcgatgat ctcgtacaag atcgcgccga gcagaaacac
      301 atccgtctgc tcggtcaccg cggcgccgcc gagcatctcc ggcgccatgt acgcgagcgt
      361 gccggcgacc tcgcgcgact ccgctgcgag cggcagccgc ccactgccat cgtctttcgt
      421 cgcgaccgcg atgccccaat cgaggacgta cacctcgccg aagcgtccga tcatgatgtt
      481 gtcgggcttc agatcgcgat ggacaatgcc gcggttgtgc gcaaacgcga tcgcgtgaca
      541 gacctgcatc agcgtcgcga ggttgtcctc gaggtcacgg ctcttcggct gccacggctc
      601 gccctcgatg cgcttgagca cgagcaacgg ccgcccacga tcgtcgctgc cgaggtcgtg
      661 cacgggcacg atgttgggat gctcgagcgc accggtaatc cacgcctcgc gcagaaggcg
      721 cggtgcagcc tcgtccgtcg cgtcgctgcg cagcgtcttg accgcgacct cgcgatgcag
      781 cgccgtttgc gtcgccttgc gcacgatcgc catgccgccc tgcccgatcg tcgcgccgag
      841 ctgcaggcgc gtcgcgagcg gatcgagctt ttcgtcgacg ccccgtgcaa cgccgtcatg
      901 tacgacggtg tctcgaagcg atggcggacg aatcgtcgcg cgcgggtcaa cgctcgcgag
      961 agtctgcgcc agagcggttt cgtccagaga cggcaggcac atgaacgtta tcagagctag
     1021 ccttgtccaa gtgcgtggcc tcctcgtcac gatgctcatc gtcgcgtgcg cagctcctcg
     1081 cggacccgca cccgcaccgc cgccattgcg cccgacgccc gtagcgcccg acgcttccgc
     1141 tgcaacgcct gtcgccgaat gccccgctcg cagcctcggc ccgacgcccg aagttgtcgt
     1201 cgtccagacg ctcgctgccg cgcagcctca cctcgcagcg agcacgccgg ccgcgaacga
     1261 aaatctcatg cagcttgccg ccgagcacgg cgctgcgttc gcggtgcgcg tcatgcgcgg
     1321 tgcggtccac ggctccgact cgatcatcta tgacatcgcc ggcctgttca tcaccgacaa
     1381 gcgtgtggtg atcgcgaagc gcgtcgccac ggacatcagc gtcatcccag gacccgacgg
     1441 gacaccgacg ccactgcaaa gcaccgtcga aaagctgtcg cccgcgatcg gccacattca
     1501 gctcgtgagt ggccacgatg tcgtcgatca cttcatcgat ctcgagcacg gcgtgcacct
     1561 gctcgcgctt acgtcgtcgt cgccgcgcgt gtggaagctc gacggcgact cgggtccgat
     1621 ctccggcgac tgccagtact ggtgggacga ctagccgaca gtcatgcggc cgccatcgcg
     1681 gagcgcagtc ggaatcagcg ggctcggctc gccggtcgat acgagccaca gctggtgagc
     1741 agcgcgcgtc acgccgatgt gcagcaggtg ccgcgcccag ttcaccgcgg ggtagctcgc
     1801 gtcgttgacg tcgaccatca cgacgtagtc gaactcgagg cctttgactt gcgcgacgtc
     1861 ggtgacgtcg acacccggct ggaagttgaa ctcgtcgcgg cggatgcggc gaagcgccgg
     1921 gacttccgcg cggcgtaggc cgtcgaagta ggcgtcggcc gtttcggcgt ggcgcgtgat
     1981 gacggcgcac gaggccgtgg gctcgcgatt catcagattg cggagcgcgt cgccgaggaa
     2041 tgcgacggct tcgccgaggt cgccgaactc gtgcagctcg accggttcgc ctgggcgtgc
     2101 ggcgagcggc tcttcgggcg cgagctcggg accgaggatc tcgcgggcga gcagcatgac
     2161 ctcggcggtc gagcggtacg agagcttcag cgggcgaacg atcgcggctt cggacttgcc
     2221 cgcgacgagc gtgacgaggt cactccagcc gctgaagttg ttgtcgaaca cgaggcgctg
     2281 cgcggtgtcg cccgcgatcg tgacggagcg ctgcgatgga tcgtcgtgcg gcgcgcgcac
     2341 ggcttcgacg aggactttga tctcgagcgc ggagcggtcc tgggcctcgt cgacggcgac
     2401 gtgctcgtag aacatctcgt cgccgcccga cggcatgagc ccgccgcgct tgagctgcgt
     2461 gaggcgcaga agcagcgggt cgtcttcgtc gtcgaagcgg ccggctggat cgtcatcgtc
     2521 gggaccgacg gctttgcctt cgctatcgag gatcgggttg ccttcttcgt cgaccggcgg
     2581 cgcttgcggc tttgcaagct ggcgcttcac ccacgcgacg aggcgctcga tgtctttctt
     2641 gctgacgtcg ctgccggcga agcccgcttc gagcgcggcc gggtctgtca tgagctcggc
     2701 ccagtcgagc acgcagtcat cggcgcgctt cttccagcgc ttgacgaagc cttcgagcgc
     2761 gacgcgcacc gtcggatcga gtgtcgtctg cttcttggtc cacgaggcaa ccgacgacag
     2821 gcgcggcacg agcgggcggt tgaccttcgc gttccacggc tcgaccgcgt cggcgcagac
     2881 ggcc
//
LOCUS       DATLBZ010000517         3644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7047495, whole genome shotgun sequence.
ACCESSION   DATLBZ010000517 DATLBZ010000000
VERSION     DATLBZ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7047495"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VMZ53_26010"
     CDS             complement(<1..624)
                     /locus_tag="VMZ53_26010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31993.1"
                     /translation="MRTTRYLVLLIALAACAKKKSASHDLDHDLIRVTSDARLRTDLV
                     GGGDFTDTATFVLVEAENTAKEGAYVTLGGDLTDEAGKTIGTLRAASLYIPSGELRTF
                     ALVDKERKPRPDAKGAKIYVRGARVPPMPPPAHIEAPRQVMDGTGSDVKVVMQGILKN
                     DAPRGGNIMVVATFFDEHGKPMTRPFSMIWVPPNGTQPVQFVGPLGSV"
     gene            complement(640..927)
                     /locus_tag="VMZ53_26015"
     CDS             complement(640..927)
                     /locus_tag="VMZ53_26015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS31994.1"
                     /translation="MKPEVMVELLENAAEQLGIKVSYEPLQTSGIGLRGGLCRVKGVY
                     RVIIDKRATTEERMTTLATSLAGFDTTELELSQKVRDLLRTYEGTGPRRAA"
     gene            complement(975..2552)
                     /locus_tag="VMZ53_26020"
     CDS             complement(975..2552)
                     /locus_tag="VMZ53_26020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS31995.1"
                     /translation="MSLTVPSEAAQLEALRGLATELPLATSEQVVVDRVLGVLASLFP
                     GRALAVRVLDVRTREPARSYVRGGGLRDSVAGEGVTLPQAALDRARLKSAVAASARVT
                     VRERWDSPFTGMATGFAIPLAAGGELYGVLDVGYPPGGDAREGDEKPMLTIASGLAFA
                     LRTLRLQEDAIGLRDYQARLLDSANALILGIDRTWRITVCNRALLELTGTTRDEVLGR
                     DIRDFISGDQRATLTTAFSAALSGQHHTAVTVMLPTRSSPVRSVWAIAPVGRAGQPGG
                     PIEAVVAIGQDQSKIDMLQQQIVRAERLATLGELAAGVVHELNNPLTSITVYAEYLVR
                     KLESQGTEPADIEKLRRIGASAQRILRFSRDLVQYARPSGKESEAVDLASVVRQSVSI
                     CEHLVERGGVDLAVEVDPELPVIQAVAGQLEQVLINLITNAVHAVDQGGKVVVRAKAE
                     GPANVLIEVADSGPGVPEADRDKIFEPFFTTKPDGKGTGLGLPIVRNIVDSHRGQISV
                     TRSDLGGAAFRVVIPIV"
     gene            complement(2549..>3644)
                     /locus_tag="VMZ53_26025"
     CDS             complement(2549..>3644)
                     /locus_tag="VMZ53_26025"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUS31996.1"
                     /translation="RITGFARDGLLGSELVDLVPIDQRAMISDAIARVLAGVNVETFD
                     LELSTTSGSPVWVSASTSTVLAGTGAVILAFRDVTAERALEHELRSTKEFLERLIDST
                     VDAIIAADFKGRIILFNQGAERLFGYRARDVIGKKPVWELYEKDGARHIMRMLRSTSY
                     GGVGRLEQTRREVRIASGETVPVSMTASVVYEGEREVATVGILTDLRERIRMEQRLLD
                     AQQKLQLSEKQALVAELAGAAAHELNQPLTSIIGYAQLIQRQSAQDAAHLRAVGIILS
                     EAERMAGIVKKIGRITKYETTDYVGGARMLDLDRAAAESDTGIPIETIEAEESKTGEF
                     SAVAPGAQVVSNTPSLDDLVDLADRSGGKP"
BASE COUNT          572 a         1212 c         1224 g          636 t
ORIGIN      
        1 cacgctgccg agcggcccga caaactgcac cggctgcgtg ccgttcggcg ggacccagat
       61 catcgagaac gggcgcgtca tcggcttgcc gtgctcgtcg aagaacgtcg cgacgaccat
      121 gatgttgccg ccgcgcggcg catcgttctt gaggatgccc tgcatgacga ccttcacgtc
      181 ggagcccgtg ccgtccatga cctggcgcgg ggcttcgatg tgagccggcg gcggcatcgg
      241 cggaacgcga gcaccgcgca cgtagatctt cgcgcccttc gcatcggggc gaggcttgcg
      301 ctctttgtcg acgagggcga acgtgcggag ctcgcccgac ggaatgtaga gcgaggcggc
      361 gcgcagcgtg ccgatcgtct tgcccgcctc gtcggtgaga tcgccgccga gcgtgacgta
      421 cgcgccttcc ttcgcagtgt tctccgcttc gacgagcacg aacgtcgcgg tgtccgtgaa
      481 gtcgccgccg ccgaccagat cggtgcggag acgcgcgtcg ctcgtcacgc ggatcagatc
      541 gtgatcgaga tcgtgcgacg cgctcttctt cttggcacag gccgcgagcg cgatcaggag
      601 cacgaggtag cgggtggtcc gcacctcgca gctatagcac tacgccgcgc gacgagggcc
      661 agtgccctca taggtgcgca gcaagtcgcg aactttttgc gacagctcga gctcggtcgt
      721 gtcgaagccg gcgagcgacg tcgcgagcgt cgtcatgcgc tcttccgtcg tcgcgcgctt
      781 gtcgatgatc acgcggtaca cgcctttgac gcgacacagg ccgccacgga gaccaatgcc
      841 gctcgtctgc agtggctcgt agctcacctt gattcccagc tgctcggcgg cgttctcgag
      901 cagctcgacc atgacttctg gcttcatccc gacagccaaa atacatggtt ggatctgggg
      961 cgcaaccttt ctgctcaaac gatcgggatc actacgcgaa atgctgctcc gccgagatct
     1021 gagcgagtta ccgagatctg accgcggtgc gaatcgacga tgtttctgac gatcggaagg
     1081 ccgaggcccg tgcctttgcc atcgggcttc gtggtgaaga acggctcgaa gatcttgtcg
     1141 cgatccgctt cgggcacgcc ggggccggaa tcggccactt cgatcaggac gttggcgggt
     1201 ccttccgcct tcgcgcggac gacgactttg ccgccttggt cgaccgcgtg gaccgcgttg
     1261 gtgatcaggt tgatcaggac ctgctcgagc tggccggcga ctgcctggat cacgggaagc
     1321 tccgggtcga cctccaccgc gagatcgacg ccgccacgct ctacgaggtg ctcgcagatg
     1381 gagacggact ggcgaacaac agatgcgaga tccaccgcct cgctctcctt gccgctcggc
     1441 cgggcgtact ggacgaggtc gcgcgagaag cgaaggatgc gctgcgcgct cgcgccgatg
     1501 cggcgaagct tctcgatgtc cgcgggctcg gtaccttgtg actccagctt tcgcacgagg
     1561 tactcggcgt agacggtgat cgaagtgagc gggttattga gctcgtgcac gacgccggct
     1621 gcgagctcgc cgagtgtggc cagccgttcg gcgcgaacga tctgctgctg cagcatgtcg
     1681 atcttcgatt ggtcctggcc gatcgcgacg accgcttcga tcgggccacc gggctgcccc
     1741 gcgcgaccga ccggtgcgat cgcccacacg cttcgaacgg gcgaggagcg cgtcggcaac
     1801 atgaccgtga ccgcggtgtg atgctggccg gagagcgcag cggagaacgc ggtcgtcagc
     1861 gtcgcgcgct gatcgccgga gatgaagtcg cgaatgtcgc ggccgagcac ttcgtcgcgc
     1921 gtcgtgccgg tcagctcgag gagtgcgcgg ttgcacaccg tgatgcgcca cgtgcgatcg
     1981 atgccgagga tgagcgcgtt ggcgctgtcg agcaggcgcg cttggtaatc gcgaaggccg
     2041 atcgcgtctt cttgcaggcg cagcgtgcgc agcgcgaatg cgaggccgct agcaatcgtg
     2101 agcatcggct tctcgtcgcc ctcgcgcgcg tcgccgccgg gtggatagcc gacgtcgagc
     2161 acgccgtaga gctcgccgcc cgccgcgaga ggaatcgcga agcccgtcgc catgcccgtg
     2221 aacggcgagt cccaccgttc gcgcaccgtg acgcgtgcac tcgcggcaac cgcgctcttg
     2281 agccgcgcgc gatcgagtgc tgcctgcgga agtgtgacgc cttcgccggc gacggaatcc
     2341 cgcaacccgc cgccgcgcac gtacgaacgt gcaggctcgc gcgtgcgcac gtcgaggacg
     2401 cgaacggcga gtgcgcgccc gggaaacagc gacgcgagca cgccgagcac gcgatcgacg
     2461 acgacctgct cgctcgttgc gagtggcagc tcggtcgcga gcccgcgcaa ggcttcgagc
     2521 tgagccgcct cgctcggcac cgtgaggctc atggtttgcc tcccgagcgg tccgcgagat
     2581 cgacgaggtc gtcgagcgac ggcgtgttcg acacgacctg cgcgccgggt gccaccgccg
     2641 agaactcgcc ggtcttgctc tcttccgcct cgatcgtctc gatcggaatg ccggtgtcgc
     2701 tctccgcggc cgcgcgatcg agatcgagca tccttgcacc gccgacgtaa tcggtcgtct
     2761 cgtacttcgt gatccgtccg atcttcttca cgatgccggc cattcgttcg gcttccgaca
     2821 ggataatgcc gacggcacgc agatgcgctg cgtcctgcgc gctttgtcgc tggatcagct
     2881 gcgcgtaacc gatgatagac gtcagcggct ggttcagctc gtgcgcggcc gcccctgcaa
     2941 gctccgcgac gagcgcctgc ttctcggaga gctgcagctt ctgttgcgcg tcgaggaggc
     3001 gctgctccat gcggatgcgt tcgcgcaagt cggtcagaat tccgacggtg gcgacctctc
     3061 gctcgccttc gtacacgacc gatgccgtca tgctcacggg caccgtctcg ccgctcgcga
     3121 tgcgcacctc gcgacgcgtc tgctcgagcc gaccgacgcc gccgtagctc gtcgagcgga
     3181 gcatgcgcat gatgtggcgc gcgccgtctt tctcgtagag ctcccacacc ggcttcttgc
     3241 cgatcacgtc gcgcgcgcgg tagccgaaca gccgctcggc gccctggttg aacaggatga
     3301 tgcggccctt gaagtcggcc gcgatgatgg cgtcgaccgt cgaatcgatg aggcgctcga
     3361 gaaactcttt cgtcgaccgc agctcgtgct cgagcgcgcg ctcggcggtc acgtcgcgga
     3421 atgcgaggat cacggcgccg gttccggcga gcaccgtcga cgtcgatgcc gagacccaca
     3481 caggcgagcc gctcgtggtc gagagctcga gatcgaacgt ctcgacgttg acacccgcga
     3541 gcaccctcgc gatcgcgtcg gagatcatcg ctcgctggtc gatgggcacg agatcgacga
     3601 gctcgctgcc gagcagaccg tcgcgcgcaa agccggtgat gcgc
//
LOCUS       DATLBZ010000518         5190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3334145, whole genome shotgun sequence.
ACCESSION   DATLBZ010000518 DATLBZ010000000
VERSION     DATLBZ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3334145"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VMZ53_26030"
     CDS             <1..243
                     /locus_tag="VMZ53_26030"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HUS31997.1"
                     /translation="VRDCLDVAFGTLGLDWQKYVELDPRYLRPTEVDHLHGDASKARE
                     KLGWKPKVTFKELIKRMVEADEHDVRSALAGRAPNM"
     gene            250..1038
                     /locus_tag="VMZ53_26035"
     CDS             250..1038
                     /locus_tag="VMZ53_26035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUS31998.1"
                     /translation="MRISLVTPSYNQAQFIGRTIDSVLSQKGDFELDYRVIDGASTDG
                     TVDILKSYGNRLQWISEADRGQVEAINKGLHAATGDVVGWLNSDDILLPGALAKVATA
                     FESNPQAEWLHGRCQIIDENDNEVRRWVSAYKHYRSKHHSLENFLTENYVSQMTVFWR
                     RSIQDEVGYLDPDIRYAFDYDLILRLAHRSAPLYIEDPIACFRWYATSKSGSGFVVQM
                     TETTEIAASSPRSSLSIRGVALAKKFAIINIYRAYGFARFKLKR"
     gene            complement(1027..2130)
                     /locus_tag="VMZ53_26040"
     CDS             complement(1027..2130)
                     /locus_tag="VMZ53_26040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUS31999.1"
                     /translation="MSRLKILFCTHTVQLGGAARSLREIIRNWDVEIDLAVPRWANAD
                     DDTQIRAFFGERVQRIHHLWLPWSEVYVGRPPIRLSARTHLLFPLTWRAQQRSFEKLV
                     RTERYDGVHLNSLVLHPMLRADAPMTFHVREILAEQHAVVRADAARARGVVFIDEATK
                     KPFEPRLPAKHIVLNNPVDMTSVGTPPPDAARKLGGNPDDLTIFTMVGTLNDEKGVPF
                     AIEAFRQVKSPDVRLVLVGSTPPVLADRIRTLAAADKRIIVWGEERNIESIYTLADYV
                     VRGEPYHCVGRTVYEALYAGCGVVIMGDAITQQLFEFERFSDRIHFYPPRNAKAFAEV
                     IEGLAGKKRTAKRGESNVERYITEFDRFIRSAL"
     gene            complement(2127..3242)
                     /locus_tag="VMZ53_26045"
     CDS             complement(2127..3242)
                     /locus_tag="VMZ53_26045"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUS32000.1"
                     /translation="MSRIGLSEPFLGGNERAYISECIDTNFVSSVGPFVERFEREFAA
                     YVGAKHAVACASGTAALHVAFRLIGLSPGDEVLVPSLTFVASVNPITYERGTPVLVDS
                     ELESWNMDPDLVVDYIERAARAGKELPKAVEVVHLLGHPAKLEAIVDVCGKHGIAVIE
                     DAAEALGARYRGGRYDGKHVGTIGRIGCFSFNGNKIMTTGGGGMLTTDDAELARRAKH
                     LTTQARLPGPAYRHDEIGYNYRLTNVAAAMGVAQLEQLPSFIAKKLAIASRYDAALAG
                     RDGFVPPPRASWASPTCWLYSLRVARGRDEQMQKLAAAQIESRPIWTPVHTMPMYATA
                     PRIGGANAEQLAAEGLSIPCSVGLSDEQQDRVISLLS"
     gene            complement(3235..4227)
                     /locus_tag="VMZ53_26050"
     CDS             complement(3235..4227)
                     /locus_tag="VMZ53_26050"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS32001.1"
                     /translation="MSVAGKIVLVTGADGFIGSHLVERLVADGAKVRAFCMYNSQGSW
                     GWLDTSTRDVKAALDVRLGDIRDPGFVADATKGVDVVMHLAALIAIPYSYNAPESFVA
                     TNVHGTLNVLEAARRHGVQRLIVTSTSEVYGTPETTPIRETHALNAQSPYAATKVAAD
                     QLSLSFQRSFGVPVVILRPFNTYGPRQSMRAVLPTILGQLLKGSSTIKLGRLDTRRDL
                     TFVADTVDGFVRAAIAQGIEGKTIQLGTGKSPSIQDVFDTACRVLGKQAKVELDNARL
                     RPDASEVLVLESDYAQATALLGWRPTVELEEGLRRHAEWMAQNLDRYDTERFHV"
     gene            complement(4224..>5190)
                     /locus_tag="VMZ53_26055"
     CDS             complement(4224..>5190)
                     /locus_tag="VMZ53_26055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HUS32002.1"
                     /translation="ETGHEIALIVDAARTLVGVLTDGDIRRALLRGAKLEDRVADHMT
                     RKFHSVGLAAGRIEVIERMQALAISQVPVVDEAGKLAGLHLLRELIGMPERSNWAIIM
                     AGGRGSRLAPLTDDVPKPMLRVAGSPILERIVLHLVNQGVKRIYLAINYLGHIIQAHF
                     GDGARFGARIEYLVEDQPLGTGGALSILPEKPTAPVLVMNGDLVTQADVGALLRYHEA
                     GTHVATVGVRKYFHTVPFGCVELDGERIIKLQEKPTLSQLVNAGIYVLAPELVTALAR
                     NTPITVPEILSNAMNRGEEVRAFEIDDDWIDVGHRDQLDQARGGS"
BASE COUNT          893 a         1736 c         1709 g          852 t
ORIGIN      
        1 gtccgcgact gcctcgacgt cgcattcggc acgctcggcc tcgactggca gaagtacgtc
       61 gagctcgacc ctcgctacct ccgcccgacg gaagtcgatc acctccacgg cgacgcgagc
      121 aaagcccgcg aaaagctcgg ctggaagccg aaggtcacgt tcaaagagct catcaagcgc
      181 atggtcgaag ccgacgagca cgacgtccgc agcgctctcg ccggccgagc gccgaacatg
      241 taaatcaccg tgcggatctc gctcgtcacg ccctcctaca atcaggcgca attcatcggg
      301 cgcacgatcg atagcgtcct atcgcagaag ggcgacttcg agctcgacta ccgcgtcatc
      361 gatggcgcga gcacggacgg cacggtcgac atcctaaaaa gctacggcaa ccggctccaa
      421 tggatcagcg aggccgaccg cggccaagtc gaagcgatca acaagggcct gcacgccgcg
      481 accggcgacg tcgtgggctg gctcaacagc gacgacatcc tcctccccgg cgcgctcgca
      541 aaggtcgcca cagcattcga atcaaacccg caagccgagt ggctccacgg ccgctgccag
      601 atcatcgacg agaacgacaa cgaagtgcgc cgctgggtca gcgcctacaa gcactaccgc
      661 agcaagcacc actcgctcga aaacttcctg accgagaact acgtcagcca gatgacggtg
      721 ttctggcgcc gcagcatcca ggacgaagtc ggctacctcg atccggacat ccgctacgca
      781 ttcgactacg acctcatcct ccgcctcgcc catcgcagcg cgccgctcta catcgaagac
      841 ccgatcgcgt gcttccgctg gtacgcgacg agcaagagcg gcagcggctt cgtggtgcag
      901 atgaccgaga caacggagat cgccgcgtcg tcgccgcgct ctagcctctc gatccgcggc
      961 gtcgcgctcg cgaagaaatt cgcgatcatc aatatctatc gcgcctacgg cttcgctcgc
     1021 ttcaagctaa agcgctgagc gaatgaaccg gtcgaactcg gtgatgtagc gctcgacgtt
     1081 ggattcgccg cgcttcgccg tgcgcttctt tcctgcgagg ccctcgatga cctcggcgaa
     1141 tgccttcgcg ttgcgcggcg ggtagaagtg gatgcggtcg gagaagcgct cgaactcgaa
     1201 gagctgctgg gtgatcgcgt ctcccatgat tacgacgccg cagccggcgt agagcgcctc
     1261 gtagaccgtg cggccgacgc agtgatacgg ctcgccgcgc acgacgtagt ccgcgagcgt
     1321 gtagatcgat tcgatattgc gctcttcgcc ccacacgatg atgcgcttgt cggcggcggc
     1381 gagcgtgcgg atacgatcgg cgagcaccgg tggcgtcgag ccgacgagga cgaggcggac
     1441 gtctggtgac ttcacctggc ggaatgcttc gatcgcgaac ggcacaccct tctcgtcgtt
     1501 gagcgtcccg accatcgtga agatcgtgag gtcgtcggga ttgccgccga gcttgcgtgc
     1561 tgcgtcggga gggggcgtgc ccacggaggt catgtcgacc gggttgttga ggacgatgtg
     1621 cttggccggc aggcgcggct cgaacggctt cttcgtcgcc tcgtcgatga acacgacacc
     1681 gcgggcgcga gcggcatcgg cgcggacgac ggcgtgctgc tcggcgagga tctcgcggac
     1741 gtggaacgtc atcggcgcgt cggcgcgcag catcgggtgc agcacgagcg agttgaggtg
     1801 gacaccgtcg tagcgctccg tgcgcacgag cttctcgaag ctgcgttgtt gggcgcgcca
     1861 ggtgagcggg aacaggaggt gcgtgcgcgc ggaaagacgg atcggcgggc ggccaacgta
     1921 gacctcgctc catggcagcc agaggtggtg gatgcgctgc acgcgctcgc cgaagaatgc
     1981 gcggatttgc gtgtcgtcgt cggcgttggc ccagcgcgga accgcgaggt cgatttcgac
     2041 gtcccagttg cggatgatct cgcgcaggct gcgcgcggca ccgccgagct gcaccgtgtg
     2101 cgtgcagaac agaatcttga gccggctcat gaaaggagcg agatcacgcg gtcctgctgc
     2161 tcgtcgctca ggccgacgga gcacgggatg ctgaggccct cggccgcgag ctgctcggcg
     2221 ttcgcgccac cgatgcgcgg cgcggtcgcg tacatcggca tcgtgtggac cggcgtccag
     2281 atcgggcgcg attcgatttg cgcggcggcg agcttctgca tctgctcgtc gcggccgcgc
     2341 gcaacgcgga gcgagtacag ccagcacgtc ggcgaggccc atgatgcccg cggtggaggc
     2401 acgaatccgt cgcggccggc gagcgcagcg tcgtagcgag aagcgatcgc gagcttcttc
     2461 gcgatgaagc tcggcagttg ctcgagctgt gcgacaccca tcgccgcggc gacgttcgtc
     2521 aggcggtagt tgtagccgat ctcgtcgtgg cggtaggccg ggcccgggag gcgcgcctgc
     2581 gtcgtcaggt gcttcgcgcg tcgcgcgagc tcggcgtcgt cggtcgtgag catgccgccg
     2641 ccgcccgtcg tcatgatctt gttgccgttg aacgagaagc agccgatgcg accgatcgtg
     2701 ccgacgtgct tgccgtcgta ccgcccgccg cgatagcgag cgccgagcgc ctctgccgca
     2761 tcttcgatca cggcgatgcc gtgctttccg cagacgtcga cgatcgcctc gagcttcgcg
     2821 gggtggccga gcaagtgcac gacctcgact gccttcggca gctccttgcc cgcgcgcgcc
     2881 gcgcgctcga tgtaatcgac cacgaggtcc ggatccatgt tccagctctc gagctcgctg
     2941 tcgacgagaa ccggcgtgcc gcgctcgtac gtgatcgggt tcaccgacgc gacgaacgtc
     3001 aacgatggga cgagcacttc gtcgcccggc gagagaccga tcaaccgaaa cgcgacgtgc
     3061 agcgccgcgg tcccgctcgc acatgcgacc gcgtgcttcg cgccgacgta cgcggcgaac
     3121 tcgcgctcga accgctcgac gaacgggccc accgacgaca cgaagttcgt gtcgatgcac
     3181 tcgctgatgt acgcgcgctc gttgccgccg aggaacggct ccgacaggcc gatcctagac
     3241 atggaaccgc tcggtatcgt accgatcgag attctgcgcc atccactccg cgtgacgccg
     3301 cagtccctct tcgagctcga ccgtcggccg ccacccgagc aacgccgtcg cctgcgcgta
     3361 gtcggactcg agcacgagca cctcgctcgc atcgggcctc aggcgtgcgt tatcgagctc
     3421 gaccttcgcc tgcttgccga gcacgcggca cgcggtgtcg aagacgtcct ggatcgacgg
     3481 cgatttgccc gtgccgagct gaatcgtctt gccctcgatg ccctgcgcga tcgccgcgcg
     3541 cacgaagcca tcgacggtgt cggcgacgaa cgtcaggtcg cggcgcgtgt cgagccggcc
     3601 gagcttgatc gtcgacgagc ccttgagcag ctggccgagg atcgtcggca gcactgcgcg
     3661 catcgattgc cgcgggccgt acgtgttgaa cggccgcagg atcacgacgg gaacgccgaa
     3721 gctgcgctgg aacgacagcg acagctgatc ggccgccacc ttcgtcgctg cgtacggcga
     3781 ctgcgcattg agcgcgtgcg tctcgcgaat cggcgttgtc tcgggcgtgc cgtagacctc
     3841 gctcgtcgac gtcacgatga ggcgctgcac gccatgacgc cgcgccgcct cgagcacgtt
     3901 gagcgtgccg tgcacgttcg tcgcaacgaa cgactccggc gcgttgtacg agtacggaat
     3961 cgcgatcagc gccgcgaggt gcatcacgac atcgacgccc ttcgtggcgt ccgcgacgaa
     4021 acccggatcg cgaatgtcgc cgagccggac gtcgagcgca gccttcacat cgcgcgtgct
     4081 cgtatcgagc cagccccacg agccttggct gttgtacatg cagaacgcgc ggaccttcgc
     4141 gccatccgcg acgagccgct cgacgaggtg gctgccaatg aagccgtcgg cgccggtgac
     4201 gagcacaatc ttgcccgcga cgctcacgag ccacctcgcg cctgatcgag ctgatcgcga
     4261 tggcccacgt cgatccagtc gtcgtcgatc tcgaacgctc gcacttcctc gccacggttc
     4321 atcgcattcg acaaaatctc gggcacggtg atcggcgtgt tgcgcgcgag cgcagtgacg
     4381 agctcgggcg cgagcacgta gatgccggcg ttgacgagct gcgacagcgt cggcttctcc
     4441 tgcagcttga tgatgcgctc gccgtcgagc tcgacacagc cgaacggcac cgtatgaaag
     4501 tacttgcgga cgccgaccgt cgcgacgtgc gtgcccgcct cgtgatagcg cagcagcgcg
     4561 ccgacatccg cttgcgtcac gagatcgccg ttcatcacga gcacgggcgc agtcggcttc
     4621 tccggcaaga tcgacagcgc gccgccggtg ccgagcggct gatcttcgac gagatattcg
     4681 atccgcgcgc cgaaccgtgc gccgtcaccg aagtgcgcct ggatgatgtg accgaggtag
     4741 ttgatcgcga gatagatccg cttcacgccc tgattgacga gatgaagcac gatgcgctcg
     4801 aggatcggac tgccggcgac gcgcagcatc ggcttcggca cgtcgtccgt cagtggtgct
     4861 agccgagagc cgcggccccc cgccatgatg atggcccagt tcgagcgctc gggcatgccg
     4921 atcagctcgc gcaagagatg caggccggcg agcttgccgg cttcgtcgac gacggggacc
     4981 tgcgagatcg cgagcgcctg catccgctcg atcacctcga tgcggcccgc tgccagtccg
     5041 accgagtgga acttgcgcgt catgtggtcg gcaacgcgat cctcgagctt cgcgccgcgg
     5101 agcaacgcgc gccggatgtc accatccgtg aggacgccga cgagcgtgcg cgctgcatcg
     5161 acaatgagcg cgatctcgtg gccggtctcg
//
LOCUS       DATLBZ010000519         4270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9618902, whole genome shotgun sequence.
ACCESSION   DATLBZ010000519 DATLBZ010000000
VERSION     DATLBZ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9618902"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VMZ53_26060"
     CDS             complement(<1..1109)
                     /locus_tag="VMZ53_26060"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HUS32003.1"
                     /translation="MTKPLFVARLAGTQSEMGAQHGALVGEEAKSLVAFYETMPERAL
                     AGDMQGAFGHVGRRVVRGIAKAWQQRLAKDRPAELVARTRAFMRAVGVENEDALLTLA
                     TMDSMQNCVSLAARGKLGPFGNPVAGRVAAAAAPACSSIVAWGDATADGELLFSRNFD
                     FPGVGVWDAAPSFVVCAPDGGQHYGFFATKGADAPVVTVVNEAGLVLAPHTRWHTGVT
                     FSGAMIVDLVHDIARRAETLEDAIKIARERPVSSTWGIAVGSAREKSALVLEIAGPHV
                     EVVRPAPGASFLVCANRYRTPALQAGQVAASSAWALHSERRERRLRQLVETRESPLTA
                     EMLVKFMGDRQDVDAPGIKRHLGGIPAQPTNVHCAV"
     gene            complement(1106..1732)
                     /locus_tag="VMZ53_26065"
     CDS             complement(1106..1732)
                     /locus_tag="VMZ53_26065"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS32004.1"
                     /translation="MPRRPDLARRAELANAAFEVLRTRGVGTSMSELADALGVKRPTL
                     YFYFPDIGAVFESVLDQTYAAHAEVLIAKLRTLDHPLDRLKALLEVTFAFHRERPHLI
                     GGLFQLWAVGGRDLTSVLEKGRKMVTASRDALVMDLRGGITRKEVLPCEPEKIVDMVL
                     AVLDGVLVHHVLGIARPEGVMDELIDRVIEPLRPSGKKQPQQKKRRRS"
     gene            1937..2539
                     /locus_tag="VMZ53_26070"
     CDS             1937..2539
                     /locus_tag="VMZ53_26070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32005.1"
                     /translation="MTREDVVIQKPEDIRFLVPVFHTSNACCGASTAYLLRRIAYRAG
                     AYAPGIQEHMFHELNTSRQGGTIEKVQRWFNGRVSSLNELGYRLQCRRVATPTPAILD
                     WVKQGKGYRGAMLPTEFKKLHPDPASAGMDSHHDMVAHAVGITMDRLDPKGDEELVMI
                     DPWPGVVGDGKDRGKVPKGLELAHKARGYHALVYFWVGWS"
     gene            complement(2549..>4270)
                     /locus_tag="VMZ53_26075"
     CDS             complement(2549..>4270)
                     /locus_tag="VMZ53_26075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32006.1"
                     /translation="VEVRVNSGANRLSVLAGTDPLVQRAVASAEISEMEGALAVSKGD
                     DAKTLRDRIAKKSVASRVISSQTSMLVLESDGDYARYGIDRKSLADVLVVGPNGIERK
                     GRTSTAVQQIAQPTTGVKNKPEVDGKTTTTKGNFATAGADDTGEMTKDAPKMAKEAEK
                     SDSFGADDARMDFDGASSQGFRGGAPGGVAGGSVNGAVAAGPSTPSATTPPPPPAPRP
                     QAAPRNERVAAQAMSEDADEEADNDDSSVRRNRNADALVQEQREAWPPKDAPVALTGE
                     FAEINKLITKKNIDGALAKARDWHAKEPGNVLALIALGDALEANGSSGTAARVYGSII
                     DLFPGRADMRRFAGERLSRIQQNAPNLIVDTYKRAVADRPDHMTGHRLYAYSLVRTGK
                     HAEAFAAILAGVDQSYRGDSYRGGDRVLAEDAGMIGAAYAAAQPSKRKEIVAQLAKRN
                     LQLATKSSTRFVLYWETDGNDVDFHIQDGRGGHAFYQNMQLPSGGELYADVTTGYGPE
                     CFAIEGTPKAGPYRLSINYYSQGPMGYGMGLLEIQRFDGKGGLSFEDRPYVIMNDHAY
                     VDLGKIK"
BASE COUNT          685 a         1477 c         1367 g          741 t
ORIGIN      
        1 accgcgcaat gcacgttggt cggctgcgcc gggatgccgc cgaggtgacg cttgatgccc
       61 ggcgcatcga cgtcttggcg atcgcccatg aacttgacga gcatctccgc ggtcagtggc
      121 gactcgcgcg tctcgacgag ctggcgcaac ctgcgctcgc gacgctccga atgcagcgcc
      181 cacgccgacg acgcggcgac ctgccctgct tgcagtgcgg gcgtgcgata gcgattcgcg
      241 cagacgagga agctcgcgcc cggcgctggc cgaacgacct cgacgtgcgg tcccgcgatc
      301 tcgagcacga gtgcgctttt ctcgcgagca gagcccaccg cgatgcccca cgtcgacgac
      361 acgggacgct cacgcgcaat cttgatcgca tcctcgagcg tctcggcgcg gcgcgcgatg
      421 tcatgcacga ggtcgacgat catcgcgccg ctgaacgtca cgcccgtgtg ccaccgcgtg
      481 tgcggcgcga ggacgaggcc cgcttcgttg acgacggtga ccaccggcgc gtcggcgccc
      541 ttcgtcgcga agaagccgta gtgctgccca ccgtcgggtg cgcacacgac gaacgatggc
      601 gctgcgtccc acacgccgac gcctggaaaa tcgaagttgc gcgagaacag cagctcgccg
      661 tcggcagtcg cgtcgcccca tgcaacgatc gacgagcacg caggcgcagc agccgcggcc
      721 acgcgtcccg cgacgggatt gccgaacggt ccgagcttgc cgcgtgcagc gagcgagacg
      781 cagttctgca tcgagtccat cgtcgcgagc gtgagcagcg cgtcttcatt ctcgacgccg
      841 accgcacgca tgaatgcacg cgtccgcgcg acgagctcgg ctgggcgatc cttcgcgagc
      901 cgttgttgcc acgccttcgc gatgccgcgc acgacgcgcc ggccgacatg gccaaacgca
      961 ccctgcatgt ctccggcgag cgcgcgctcc ggcatcgttt cgtagaacgc gacgaggctc
     1021 ttggcctctt caccgacgag cgctccgtgc tgcgcgccca tctcggactg cgtgcccgcg
     1081 aggcgagcga caaacaatgg cttggtcatg aacgtctcct cttcttctgc tgcggctgct
     1141 tcttgcccga cggacgcagc ggctcgatca cgcgatcgat cagctcgtcc atcactcctt
     1201 caggccgtgc gatgccgagc acgtgatgca cgagcacgcc gtcgagcacg gcgagcacca
     1261 tgtccacgat cttctcgggc tcacacggca acacttcttt gcgcgtgatg ccgccgcgca
     1321 gatccattac gagcgcgtcg cgcgacgcgg tcaccatctt gcggcccttc tcgagcacgc
     1381 tcgtcaaatc gcggccgccg accgcccaca gctgaaacag cccgccgatc agatgcggtc
     1441 gctcgcggtg aaacgcaaac gtcacctcga gcaacgcctt cagccgatcg agtgggtggt
     1501 cgagcgtgcg cagcttcgcg atcaagacct ccgcatgcgc ggcatacgtc tggtcgagca
     1561 cgctctcgaa caccgcgccg atgtccggaa agtagaagta gagcgtcggt cgcttcacgc
     1621 cgagcgcatc ggccagctcg ctcatgcttg tccctacgcc tcgcgtccgc agcacctcga
     1681 acgcggcgtt cgccagctcg gctcgtcggg caaggtcggg gcggcgcggc ataacctgac
     1741 actctgtcag tacaagaagg ctgacaacct gtcaaggtat ggcgtgggaa ggccggtccc
     1801 tccggagacg tatgaggtct gtgggggcgg ccaccagaac attgcgcaac ctcgcgcaca
     1861 acctcagatc ggtggcgcgc attcaccccg cggcgatctc agtcgtgtac gcttcaagac
     1921 gctcgtgggg ctagcgatga ctcgcgaaga cgttgtcatc cagaaaccgg aagacattcg
     1981 gttcctggta ccggtgtttc acacgtcgaa cgcgtgctgc ggcgcctcga ccgcatatct
     2041 gctccgccgc atcgcctacc gcgctggcgc gtacgcaccc ggcatccagg aacacatgtt
     2101 ccacgagctg aacacgtcgc gccaaggcgg gacgatcgag aaggttcagc gctggttcaa
     2161 tggccgcgtg tcgagcctca acgagctcgg ctatcgcctg caatgccgcc gcgtcgccac
     2221 gcccacgccc gcgatcctcg actgggtcaa gcaaggcaaa ggctatcgcg gcgcgatgct
     2281 gccgaccgag ttcaagaagc tgcatcccga cccggcgtcc gccggcatgg actcgcacca
     2341 cgacatggtc gcgcacgccg tcggcatcac gatggaccgc ctcgatccca agggcgacga
     2401 agagctcgtc atgatcgacc cgtggcccgg cgtggtcggc gacggcaaag atcgcggcaa
     2461 ggtccccaag ggcctcgagc tcgcgcacaa agcgcgcggc tatcacgcgc tcgtgtactt
     2521 ctgggtcggc tggtcctgag ccttctagct acttgatctt gccgaggtcg acgtacgcgt
     2581 ggtcgttcat gatcacgtac ggacgatctt cgaacgagag cccgcctttg ccgtcgaagc
     2641 gctggatctc gagcaggccc atgccgtagc ccatcgggcc ctgcgagtag tagttgatcg
     2701 agagccgata cggaccggcc ttcggcgtgc cttcgatcgc gaagcactcg gggccgtagc
     2761 ccgtcgtaac gtccgcgtag agctcgccgc ccgacggcag ctgcatgttc tggtagaagg
     2821 cgtggccgcc gcgcccgtcc tggatgtgga agtcgacgtc gttaccgtcg gtctcccagt
     2881 agaggacaaa gcgcgtcgac gacttcgtcg cgagctggag gttgcgcttc gcgagctgcg
     2941 cgacgatctc cttgcgcttg ctcggctgcg cggcagcgta cgcagcgccg atcatgcccg
     3001 cgtcttccgc gagcacgcgg tcgccaccgc ggtagctgtc accgcgatac gactggtcga
     3061 cgcccgcgag gatcgcggcg aacgcttcgg cgtgcttgcc cgtgcgcacg agcgagtacg
     3121 cgtagaggcg atggcccgtc atgtggtcgg gacgatccgc gaccgcgcgc ttgtacgtat
     3181 cgacgatcag gttcggcgcg ttctgctgga tccgcgacag acgctcgccc gcaaagcggc
     3241 gcatatcagc gcgaccgggg aagaggtcga tgatcgagcc atagacgcgc gccgccgtgc
     3301 cggagctgcc attggcttcg agcgcgtcac cgagcgcgat cagcgcgagc acgttgccgg
     3361 gctctttcgc gtgccagtcg cgagccttcg cgagtgcgcc gtcgatgttc ttcttcgtga
     3421 tgagcttgtt gatctcggcg aactcgccgg tcaacgcgac cggtgcatcc ttcggcggcc
     3481 acgcttcgcg ttgctcttga acgagcgcgt ccgcgttacg gttccggcga acgctgctgt
     3541 catcgttgtc agcctcctcg tcggcgtctt cgctcattgc ctgcgcagct actcgctcgt
     3601 tgcgaggcgc agcctgtggt cggggagcgg gcggcggcgg tggagtcgtg gcagagggcg
     3661 tcgacgggcc ggctgcgacc gcaccgttca cggagccacc agcaacgccg cccggcgcac
     3721 cgccgcggaa cccttgcgag ctcgcaccgt cgaagtccat gcgcgcgtcg tcagcgccga
     3781 agctgtcgga cttctctgct tccttcgcca tcttcggcgc gtccttcgtc atctcgcccg
     3841 tgtcgtcggc gccggcggtc gcgaagttgc ccttcgtcgt cgtcgtcttg ccgtcgactt
     3901 cgggcttgtt cttcacgccg gtcgtcggct gcgcgatctg ctgcaccgcc gtcgatgtgc
     3961 ggcccttgcg ctcgatgccg ttgggaccga cgacgagcac atccgcgagc gacttgcgat
     4021 cgatgccgta gcgcgcgtag tcgccatcgc tctcgaggac gagcatcgac gtctggctcg
     4081 agatgacgcg cgacgcgacc gacttcttcg cgatgcgatc gcggagcgtc ttggcgtcgt
     4141 cgcccttact caccgcgagc gcgccttcca tctcgctgat ctccgctgac gcgacggcgc
     4201 gctgaaccag cggatccgta ccagcgagaa cactgagccg attcgcgccg ctgttgacgc
     4261 gaacctcgac
//
LOCUS       DATLBZ010000520         2755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2915384, whole genome shotgun sequence.
ACCESSION   DATLBZ010000520 DATLBZ010000000
VERSION     DATLBZ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2915384"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VMZ53_26080"
     CDS             <1..903
                     /locus_tag="VMZ53_26080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32007.1"
                     /translation="EALGRVRRSPHYQRAREEVGGELRYYVDELGQRLDALEQLFGSG
                     KRRPARTDDDDEHEGEVRLHQKRTPRPPASRDALIAGIAEALLLERSIHTIADPDAHS
                     ATVVVSRIGAAKGDGLIAVTQCYVHLGSQWFDNGAHANDTGEIGAINNVKGKGSFDDW
                     QKFWRARRDCAVVLRGLFVRDAIANDHGTWMLSAASAEPEVIRVEVRPGGAVSAADTL
                     KQHIAARRELERVLDQGGALPPNPDVLLPVTRTISARSPMLFWHKPYDVELEDFATGW
                     IDRTSVKDVRSLVERCWHLAWSRT"
     gene            900..>2755
                     /locus_tag="VMZ53_26085"
     CDS             900..>2755
                     /locus_tag="VMZ53_26085"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS32008.1"
                     /translation="MSRPSFRVFVTRHHGGLTSAVLLRRYKLLFEPPPPAAMAEDLDT
                     ALTRLAPQAALIADNAENAERYMWTEELDHRRIEVDIHPGRPDPRGYVISGSTVPIRL
                     GYASAKLDKASLYRIVVPRFDWSFVAEDLDAVPDTIRALVFSSLVGDATASLYDLRRE
                     IEESVIEWSPVEAAGRVKKRSGEEPDPAPTLEAVAEDWVGLARAGRLPPTVGTDAVYD
                     TLYSIFDDKKVPSLLFVGPRGAGKSALVRRIARGLLDRSRGKGAKRRLWATSADRIVA
                     GMVYLGMWQQRCLAMVKELEGGVDVLYIDRLADAMAPMSDGASIIDILAGPIISGELS
                     LFAECDEAELVRARQRFPALVDSLRVIRVPEATPAQVMALLEPYGQRLDPPVSLKIDA
                     SRRLVELLAAFRRDTAFPGKAIQFVDYCSLHKHTSLSVGDLTNTFGKWSGLPVDLLAP
                     ERALDTAAISRELQKGVIGQDAACTVAARVIARLKAGLDDPQRPVGSLLFAGPTGVGK
                     TELAKQLARYLFGDADRLVRIDMSEITTPGATARLITPSPAGTSLADRIRRQPLSVVL
                     FDEIEKADDAAFDLLLGVLGEGRLTDALGRLVDFRMSLIVMTTNLGAADPKPA"
BASE COUNT          448 a          986 c          872 g          449 t
ORIGIN      
        1 gaggcgctcg gtcgagttcg ccgctcgccg cattaccagc gcgcccgcga ggaggtcggc
       61 ggcgagctgc gctactacgt cgacgagctc gggcaacgcc tcgatgcgct cgagcagctg
      121 ttcggcagcg gcaagcgccg gcccgctcgc accgacgatg acgacgagca cgagggcgag
      181 gtgcggctgc atcagaagcg gacgccgcga ccaccggcca gccgcgatgc attgatcgcc
      241 ggaatcgccg aggcgctgct gctcgagcgg tcgattcaca cgattgcgga tccagacgcg
      301 cattctgcaa cagtcgtcgt ctcgcgtatc ggcgcagcga aaggcgacgg cctcattgcg
      361 gtcacccagt gctacgtcca cctcggctcg cagtggttcg acaacggcgc gcacgcgaac
      421 gacaccggcg aaatcggtgc gatcaacaac gtcaagggca agggttcctt cgacgactgg
      481 cagaagttct ggcgcgctcg tcgcgattgc gcggtcgtgc tgcgcggcct gttcgtgcgc
      541 gatgcgatcg caaacgatca cggcacgtgg atgctcagcg ccgcgtccgc cgagccggaa
      601 gtgattcgcg tcgaggtgcg ccccggtggc gcggtctcag ccgccgatac gctcaagcaa
      661 cacatcgccg cgcgtcgcga gctcgagcgc gtcttggatc agggtggcgc gctgccgccg
      721 aatcccgacg tgctgctccc cgtcacgcgg acgatcagcg cgcggtcgcc gatgttgttc
      781 tggcacaagc cgtacgacgt cgagctcgag gacttcgcga ccggctggat cgatcgcacg
      841 tcggtcaaag atgtgcgcag cctcgtcgag cgctgctggc acctcgcgtg gagtcgaacg
      901 tgagccgccc gagctttcgc gtattcgtga cgcgtcacca cggtggcctg acgtcggccg
      961 tgctgctgcg tcgctacaag ctgctgttcg agccgccgcc acccgccgcg atggccgaag
     1021 acctcgacac cgcgctcaca cgtctcgcac cgcaagcagc gctcattgcc gacaatgcag
     1081 agaacgccga gcgctacatg tggaccgagg agctcgacca ccggcgcatc gaagtcgaca
     1141 tccatccggg gcgcccggat ccacgcggct acgtcatctc cggctcgacg gttccgattc
     1201 ggctcggcta cgcatccgcg aagctcgaca aggcgtcgct gtatcgcatc gtcgtgccgc
     1261 gcttcgattg gtcgttcgtt gccgaagatc tcgacgcggt gcccgacacg atccgcgcgc
     1321 tcgtcttcag ctcgctcgtc ggagacgcga ctgcatcgct ctacgacctg cgtcgcgaga
     1381 tcgaagagtc ggtgatcgag tggagccccg tcgaagcggc gggccgcgtc aagaaacgca
     1441 gcggcgaaga gcccgacccc gcccccacgc tcgaggccgt cgccgaagac tgggtcggtc
     1501 tcgcgcgagc aggtcgcctg ccaccgaccg tcggcacgga cgccgtctac gacacgctct
     1561 actcgatctt cgacgacaag aaggtgccgt cgctcctgtt cgtcggcccg cgcggcgcag
     1621 gcaagtccgc gctcgttcgc cggatcgcgc gtggcctcct cgatcgcagc cgcggtaaag
     1681 gcgcaaagcg ccgcctgtgg gcaacgtccg cggaccgcat cgtggcgggc atggtctatc
     1741 tcggcatgtg gcagcagcgc tgcctcgcca tggtcaagga gctagaaggc ggcgtcgacg
     1801 tcctctacat cgatcggctc gcggacgcga tggcgccgat gtccgatggc gcatcgatca
     1861 tcgacattct cgcgggcccc atcatcagcg gcgagctctc gctgttcgcc gaatgcgacg
     1921 aagccgagct cgtgcgcgcg cgccaacgct tccctgccct cgtcgactcg ctgcgcgtga
     1981 ttcgcgtccc cgaagcgaca ccggcgcaag tgatggcgct gctcgaacca tacggccagc
     2041 ggctcgatcc tcccgtctcg ctcaagatcg atgcctcgcg ccgcctggtc gagctgctcg
     2101 ctgcattccg tcgcgacaca gcgttcccgg gcaaagcgat ccaattcgtc gactactgct
     2161 cgctgcacaa gcacacctct ctctccgtcg gcgatctcac caacacgttc ggcaagtggt
     2221 caggccttcc cgtcgatctg ctcgccccag agcgcgcact cgacaccgcc gccatctctc
     2281 gcgagctcca gaaaggcgtg atcggccaag acgccgcatg cacggtcgcg gctcgcgtca
     2341 tcgcgcgcct caaggcaggc ctcgacgatc cgcagcgtcc ggtcggctcg ctgctgttcg
     2401 cgggccccac gggcgtcggc aagaccgagc tcgccaaaca gctcgcgcgc tacctgttcg
     2461 gcgatgctga ccggctcgtc cgcatcgaca tgtccgagat cacgacgccc ggcgcgaccg
     2521 cgcgtctcat cacgccgtcg ccggcaggca catcgctcgc ggatcgcatc cgacgtcagc
     2581 cgctctccgt cgtcctgttc gacgagatcg agaaagcgga cgacgccgcg ttcgatctac
     2641 tcctcggcgt gctcggcgaa ggtcgcctca ccgatgcgct cggtcgcctc gtcgacttcc
     2701 gcatgtcgct catcgtgatg acgacgaacc tcggcgcggc cgatccaaag cccgc
//
LOCUS       DATLBZ010000521         7153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1428224, whole genome shotgun sequence.
ACCESSION   DATLBZ010000521 DATLBZ010000000
VERSION     DATLBZ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428224"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VMZ53_26090"
     CDS             complement(<1..374)
                     /locus_tag="VMZ53_26090"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32009.1"
                     /translation="MPVEAPDRVGSVIDGRYKITEVMASGAMGAVYKAERVPVGKLVA
                     VKFLHASFANDSEFQARFERETRVMSKLNHPNCVSVLDFGVWEGSPYLVMDYVDGVTL
                     RTRLDKQQKLPALEALQVARQML"
     gene            complement(398..763)
                     /locus_tag="VMZ53_26095"
     CDS             complement(398..763)
                     /locus_tag="VMZ53_26095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32010.1"
                     /translation="MATTLTFDKFWQWLGGHANCIVRAGTPESVLIDHDDFHWTLISE
                     DDHTKVVQLARAKDLVGEILVFSTEIAYVQVEPSETEGEWLFECLIENEKAREVAYHF
                     VMAHEYDDGEHRREEKWTH"
     gene            complement(835..2070)
                     /locus_tag="VMZ53_26100"
     CDS             complement(835..2070)
                     /locus_tag="VMZ53_26100"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS32011.1"
                     /translation="MRLLPFVVLMALTTPAVADQKAAEKLFRAGERSYKAQKFLSAAR
                     NFEEAYKELPAPEIAFSAAQAYRRQYRVDPDHAEYAELAVKYYKIYLNDAKDSGTRLG
                     DAADSLGEMARVLERVGTTGKLTTSQEKKTQLGVSIVLADAQVATKPSGMHEVDDDKD
                     DDKADDKKLVIKASIDGKPVDLDTLTDVSPGDHTIHAEADGYLPADQPAHIAAGSWKY
                     EDVTLRPKPANVTVKTEAGAAISVDHLGMGTAPRAPLQIEGGTHVITVTRLGRRPVAR
                     EIRVTRDQQLTLDVDLEATTRRRAVPWVIGVGGGVTLGFTGFFLIGAKYADNRASDRL
                     AELMTGNQDRSVLDNYNTWRHRRDLAINLAIVSGGAAVAVGLTAAWMYYFDTPSAEGV
                     TVTPWTSREGAGAAVSGRF"
     gene            complement(2067..4994)
                     /locus_tag="VMZ53_26105"
     CDS             complement(2067..4994)
                     /locus_tag="VMZ53_26105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32012.1"
                     /translation="MRALAFAALLLTGVAATGCSNLPAFEQTCGNGAIDPGESCDRAN
                     DSTCQECVQTCPGHRDEECTETAGPGYRCGANDMCFAPSGLFLQESVKEIDFPVGGGQ
                     IADLDGDRIGDIVGYSDINLDVRFGVPSFNIETRSSVIVPFSNISLAVRAFPAYDDEK
                     TGERVAARSDILLPTQDGLAGFTARAQSLVSYPFANAPGQRGVCALDPDPTTHIPRAV
                     PVQAFSLDKHHLAFVRRRVQSGALEIAILDPANAGADQCSVQPLCSLSTDSAQPGKYV
                     VLYDRYDLQPTATQRTSTIIAVSALAKYDVPDEMKSKTADVCVVHFADDTNVAAGSHF
                     KFQTLLGNATIGRDAFVTLARTHKTAACPALYTSAPALGVQVFAPALTAPCTYGAPQA
                     VPTYLTPGTFVTGRIPLVPPIPNYAPDAIAVVSETSSASGSTDIFAFQNPTGFPNATE
                     TEGKKVLSHVTRPLVHFRSADLDGDGRVEGVGAVSLPLAGVFGGQPDFDILYRTKTTD
                     SFLTYHMATLSIPSHIELGDFDGNGLTDIAFSERLGSEDRLLVSYGTPDRPLDPIEVA
                     TFTAVGSLQRIDLPDSLDPTGEALDDLVVVDAVNPPEVNLAILHGNPQRTMLAFYDPN
                     NNLTSPTKLVAAIAGNFVPDGDAMETIVDVSLFDDAEPNNMYVVSGILHAQGLRDPAL
                     AFKVPALTNQCPFGAPAGKVCVLGSKFRTWSLGNRDVIIAINPPIASGPTAPPLMPTR
                     KLVTLDPMVSVPGASERSIDAQFSAATVDPDQLAIDMVAADVYGTGQRELFISMGSIP
                     GFMGDPEKDHVAMCTVDASGVPQMCTNILASIPDPSLDAAWRCLAAQPARVHPEGRRD
                     FVPEIKSDDLVLLCRRPATNERGPEGRVLRISRDPESPDPALRSETVLSGPGVAQFQF
                     LAVGDVNGDRVDDLVGISFDQKTQLPKLHIYPQCHAEDLKCHEAAITHGAALGAEDRG
                     AMP"
     gene            complement(4994..6631)
                     /locus_tag="VMZ53_26110"
     CDS             complement(4994..6631)
                     /locus_tag="VMZ53_26110"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32013.1"
                     /translation="MSTVNTGNTPGMAVGANDQDLAPGQEVGEYRIENVLGQGGFGTV
                     YAAVHPLIGKRAAIKVLARKYSADPTVVSRFVAEARAVNQIRHRNIIDIFAFGQLADG
                     RHYYVMEYLEGQPLDAYLRDRGPLTLDEALPILRAIARALDAAHGKGIAHRDLKPENI
                     FLAHDTDGDTYPKLLDFGIAKLMAPEDDVRHRTGTGVPIGTPYYMSPEQCRGKDVDHL
                     TDVYSFGIVVYRMLTGTFPFEGDDYVELLYKQVNEEAAPPSTRNASLPASVDAAIAWM
                     MQKDRTKRPPTVVEAVHALDPDASRTPSLPGAVKTGRGSRPSIPPKRQVTPDALAATT
                     APDQAPVALPSAPTMAASSQLPTGTGDAKLPTAPASGGKGLIVAILAVLVLAGAGVAA
                     YVLVVAKKTPDATKDVVELPPVGSGSQSLPLATPVDAAVVAEAIDAAAPTPSSVLITL
                     EGVPKGTQVFRNGARVGLAPKFEIAYGTKEVPLELRAPSYEPTPLVIVPNADQTQTVT
                     LTKTKTSHSQHTQPTDVPPDKDSLADPFAKKKTKSGD"
     gene            complement(6656..>7153)
                     /locus_tag="VMZ53_26115"
     CDS             complement(6656..>7153)
                     /locus_tag="VMZ53_26115"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS32014.1"
                     /translation="DDDAKILVDGRALATAPAAALEVPHGKHLLSVLRRGREPFAQEV
                     TVGRGQELRIDAELRRTGQRKAVPWVYGGAGLAAAGAITTALFARSRNSHAEDLQASI
                     EMGNQPVSVADELERTVRSRDRFVMWTWILGGAAIAAGATGTALLVLDTPTSDSAVVG
                     VTGSF"
BASE COUNT         1181 a         2243 c         2401 g         1328 t
ORIGIN      
        1 agcatctgac gcgcgacctg gagcgcttcg agcgccggca gcttctgctg cttgtcgagg
       61 cgcgtgcgca acgttacgcc gtcgacgtag tccatcacca gatacggcga gccttcccac
      121 acgccgaagt ccaagaccga aacgcagttg gggtgattga gcttgctcat cacgcgcgtc
      181 tcgcgctcga accgcgcttg gaactcgctg tcgttcgcaa acgacgcgtg gaggaacttc
      241 accgccacga gcttgccgac tggcacgcgc tccgccttgt agacggcgcc catggcgccc
      301 gaggccatga cctcggtgat cttgtagcgc ccgtcgatga cgctaccgac ccgatcaggt
      361 gcttcgacgg gcatgcgagc ccgtcgaatc tagcacgtca gtgcgtccac ttctcttcgc
      421 gacggtgctc gccgtcgtcg tactcgtgcg ccatcacaaa gtggtaagcg acctcgcgcg
      481 ccttctcgtt ctcgattagg cactcgaaca gccactcgcc ttcggtctcg ctgggctcga
      541 cctgcacgta tgcgatctcc gtcgagaaga cgaggatctc gccgaccaga tcctttgcgc
      601 gggctagctg cacgaccttc gtgtggtcgt cctcggagat gagcgtccag tgaaaatcgt
      661 cgtgatcgat gagcacggat tcgggcgtac cggcgcgcac gatgcagttg gcgtggccac
      721 cgagccactg ccagaacttg tcgaacgtca atgtcgtcgc cacagccgct agtcgcttac
      781 ccgtcgaacc catacgcgcg gcttatagcc gcagttttcg ccgccgccaa gggctcaaaa
      841 ccgtcctgaa accgccgccc ctgcgccctc ccgcgacgtc cacggcgtca ccgtgacgcc
      901 ctcggcggaa ggagtatcga agtaatacat ccatgccgcc gtcagcccga ccgcgacggc
      961 agcgccaccc gacacgatcg cgagattgat ggcgagatcg cggcgatgcc tccacgtgtt
     1021 gtagttgtcg agaacagagc ggtcctgatt tcccgtcatg agctccgcga gacgatcgct
     1081 cgcgcggttg tctgcgtact tggcgccgat gagaaagaag cccgtgaagc cgagcgtcac
     1141 gcctccaccg acgccgatga cccatggcac cgcgcggcgg cgcgtggtcg cctctagatc
     1201 gacatcgagc gtcagctgtt gatcgcgcgt cacgcgaatc tctcgcgcaa cgggacgccg
     1261 tccgaggcga gtcacggtga tgacgtgcgt gccgccttcg atctgcagcg gcgcgcgcgg
     1321 agctgtgccc atgccgaggt gatcgacgct gatcgccgcg cctgcctcgg tcttgacggt
     1381 gacgttcgcc ggctttggac gaagcgtgac gtcctcgtat ttccacgacc cggccgcgat
     1441 gtgcgctggt tgatccgcag gcaggtagcc atccgcttcg gcgtggatcg tgtgatcgcc
     1501 ggggctgaca tcggtgagtg tatcgagatc gaccggtttg ccatcgatcg acgctttgat
     1561 gacgagcttc ttgtcatcag ccttgtcgtc gtccttgtca tcgtcgacct cgtgcatgcc
     1621 gctcggcttc gtcgccactt gcgcatccgc gagcacgatg ctgacgccga gctgcgtctt
     1681 cttctcctgc gaggtcgtga gcttgccggt cgttccgacg cgttcgagga cgcgcgccat
     1741 ttcgccgagg ctgtcggcgg cgtcgcccag gcgcgtcccc gagtccttcg cgtcgttgag
     1801 atagattttg taatacttca ccgcgagctc ggcgtactcg gcgtggtcgg ggtcgacgcg
     1861 gtactgccgg cgatacgcct gcgcggcgga gaacgcgatt tccggcgcgg gaagctcctt
     1921 gtaagcctcc tcgaagttgc gcgcggcgct gagaaacttc tgcgccttgt acgagcgctc
     1981 gccggcgcga aacagcttct cggctgcctt ttgatcggcg acggctggcg tggtcagtgc
     2041 catcaggacg acaaatggga ggagtctcat ggcatcgccc cccggtcttc ggcgccgagc
     2101 gcggcaccat gcgtgatcgc cgcttcgtga cacttcaggt cctcggcatg gcactgcgga
     2161 tagatgtgga gcttgggcag ctgcgtcttt tgatcgaagc tgatgccgac gagatcatct
     2221 acgcggtcgc cgttgacgtc accgacggcg aggaattgga actgcgcgac gcctggacca
     2281 gagagcacgg tctcggaacg cagagctggg tcgggcgact cgggatcacg cgagatgcga
     2341 agcacgcgtc cctctggccc gcgttcgttt gtggcggggc gtctgcacaa gagcactagg
     2401 tcgtcgcttt tgatctccgg cacgaagtcg cgccgaccct cgggatggac gcgcgcgggc
     2461 tgtgccgcca agcatctcca cgccgcgtcg agcgacggat cggggatgga cgcgaggatg
     2521 ttcgtgcaca tctgcggcac gccgctcgcg tcgacggtgc acatcgcgac gtggtccttc
     2581 tcggggtcgc ccatgaagcc gggaatcgag cccatcgaga tgaagagctc tcgttgcccc
     2641 gtgccgtaga catctgccgc gaccatgtcg atcgcgagct gatcgggatc gaccgtcgcc
     2701 gcggagaatt gggcgtcgat cgacctttcg gacgcgcccg gaacggaaac catcggatcg
     2761 agcgtgacga gcttgcgagt cggcatcagc ggtggcgcgg tcgggcctga cgcgatcggc
     2821 ggattgatcg caatgatgac gtcgcggttg ccgaggctcc acgtcctgaa cttcgagccc
     2881 agtacgcaaa ccttgccggc gggcgcaccg aatgggcatt ggttcgtgag cgcgggcacc
     2941 ttgaacgcca gcgccggatc gcgcagcccc tgcgcatgga gaatgccgct gacgacatac
     3001 atgttgttag gctcggcgtc gtcgaacaag gagacgtcga cgatcgtctc catcgcatcg
     3061 ccgtccggga cgaagtttcc tgcgatcgcg gcgacgagct tcgtcgggct cgtcaggttg
     3121 ttgttgggat cgtagaacgc gagcatcgtg cgctgcgggt tgccgtgcag gatcgcgagg
     3181 ttgacctcgg gcggattgac ggcatcgacg acgacgagat cgtcgagcgc ttcgccggtc
     3241 ggatcgagcg agtccggcag atcgatacgc tgcagacttc ccacggcggt gaatgtcgcg
     3301 acctcgatgg gatcgagcgg gcggtccggc gtcccatacg agacgagcag acgatcctcg
     3361 gagccgagcc gctcggagaa ggcaatatcg gtcaggccat tgccgtcgaa gtcaccgagc
     3421 tcgatgtggc tcggaatgct caacgtggcc atgtggtacg tgagaaagct gtcggtggtc
     3481 ttggtgcggt agaggatgtc gaagtccggc tggccgccga acacgccagc gagtggaagc
     3541 gacaccgcgc cgacgccctc gaccctgcca tctccatcga ggtccgcgga tcgaaagtgc
     3601 acgagtgggc gggttacgtg cgagaggacc ttcttgccct cggtctcggt cgcgtttgga
     3661 aaccccgtcg gattttggaa cgcgaagatg tccgtcgagc cgctcgcgct actcgtctca
     3721 ctgacgaccg caatcgcatc gggtgcgtag tttgggatcg gcggaacgag cgggatgcga
     3781 ccggtgacga acgtgcccgg cgtcaggtac gtcggtacgg cttgcggcgc accgtacgtg
     3841 cacggtgccg tgagggccgg cgcaaagacc tgcacgccga gcgctggcgc agacgtgtag
     3901 agcgcgggac atgcagcggt cttgtgtgtc cgcgcgagcg tcacgaacgc atctcggccg
     3961 atggtcgcgt tgcccaagag cgtttgaaat ttgaagtgcg agcccgcggc gacgttcgtg
     4021 tcgtctgcga agtgaacgac acagacgtcg gcggtcttcg atttcatctc gtcgggaacg
     4081 tcgtactttg cgagcgccga cacggcgatg atcgtcgacg tgcgttgcgt ggccgtcggc
     4141 tggaggtcgt agcggtcata gagcaccacg tacttgccgg gctgcgccga gtccgtgctg
     4201 agactgcata gcggctgaac ggagcactga tccgcaccgg cattcgcggg atcgaggatc
     4261 gcgatctcga gcgcgccgct ctggacgcga cggcggacga acgcgaggtg atgcttgtcg
     4321 agcgagaacg cttgcaccgg cacagcgcgc ggaatgtgcg tggtcggatc tggatcgagc
     4381 gcacatacgc cgcgttggcc aggcgcattc gcaaacggat acgagacgag agactgcgcg
     4441 cgagccgtga agcccgcgag cccatcctgc gtcggcaaca agatatcgga gcgcgccgct
     4501 acacgttcgc cggtcttttc gtcgtcgtag gcggggaacg cgcgcacggc gaggctgatg
     4561 ttcgaaaacg gcacgatgac gctgctgcgc gtctcgatgt tgaagctcgg gacgccgaac
     4621 ctcacgtcga ggttgatgtc cgagtagccg acgatatcgc cgatgcgatc cccatcgagg
     4681 tccgcgatct gaccgccacc gacgggaaaa tcgatctcct tgacggactc ttgcaagaac
     4741 aggccgctcg gcgcgaagca catgtcgttc gcaccgcagc gatagcccgg tccggcagtc
     4801 tcggtgcact cctcgtcccg atgaccagga cacgtctgca cgcactcctg acaggtcgag
     4861 tcgttagccc ggtcgcacga ctcgccggga tcgatcgcgc cgttgccgca cgtttgctcg
     4921 aacgcgggga gattggagca tccggtggcg gcaacaccgg ttaacaacag cgccgcgaag
     4981 gcgagtgcgc gcatcagtct cccgatttgg tctttttctt cgcaaacgga tcggcgagcg
     5041 agtctttgtc gggtggcaca tccgttggct gcgtgtgctg cgaatgcgat gtcttcgtct
     5101 tcgtcagcgt cacggtctgt gtttgatctg cgttcggaac gatcacgagc ggcgtgggct
     5161 cgtagctcgg cgcccgcagc tcgagcggta cttccttcgt gccgtacgca atctcgaact
     5221 tcggcgcgag accgacgcgc gcgccgttgc gaaacacctg cgtgcccttc gggactccct
     5281 cgagggtaat caagacggag gatggcgtcg gcgcggctgc atcgattgcc tccgccacca
     5341 cggccgcgtc gaccggcgtt gcgagcggca gcgactgcga gccgctacca accggtggaa
     5401 gctcgacgac gtccttcgtc gcatcgggcg tcttcttggc gacgacgagc acgtatgcag
     5461 cgacgcctgc accggcgagc acgagcaccg cgagaatggc gacgatcaag cctttgccac
     5521 cggaagccgg cgcggtcggt agcttcgcgt cgccggtgcc ggtcggtagc tggctcgatg
     5581 cggccatcgt cggtgccgac ggaagcgcga cgggtgcttg atcgggtgct gtcgtcgcag
     5641 cgagcgcgtc cggcgtgacc tggcgcttcg gcgggatcga cgggcggctg cctcgtccgg
     5701 tcttcacggc gcccggcagc gatggcgtgc gcgacgcatc gggatcgagc gcgtgcactg
     5761 cttcgacgac ggtcggcggc cgcttcgtgc gatctttctg catcatccac gcgatcgcgg
     5821 cgtcgacgga tgcgggaagg ctcgcgttgc gcgtgctcgg cggcgctgct tcttcgttga
     5881 cctgcttgta cagcagctcg acgtagtcat cgccttcgaa cggaaacgtc cccgtgagca
     5941 ttcgatagac gacgatgccg aacgagtaga cgtcggtgag gtgatcgaca tctttgccgc
     6001 ggcactgctc ggggctcatg taatacggcg tgccgatagg gacaccggtc cccgtgcgat
     6061 gccgaacgtc gtcctcgggg gccatgagct tcgcgatgcc gaagtcgagg agcttgggat
     6121 aggtgtcgcc gtcggtatcg tgcgcgagga agatgttctc gggtttgaga tcgcgatgcg
     6181 cgatgccttt gccgtgtgcg gcatcgagcg cacgggcgat cgcgcgcagg atcggcagtg
     6241 cttcgtcgag cgtgagtggc ccgcgatcgc gcaggtacgc gtcgagcggc tggccctcga
     6301 ggtactccat cacgtagtag tgacggccgt cggcgagctg gccgaacgcg aagatgtcga
     6361 tgatgttgcg atggcggatc tgattgacgg cgcgcgcttc cgcgacgaag cggctgacga
     6421 ccgtgggatc ggcggagtac ttgcgtgcga ggactttgat cgcggcgcgc ttgccgatca
     6481 gcggatgcac cgcggcgtag accgtgccga agccgccctg cccgagcacg ttctcgatgc
     6541 ggtactcgcc gacctcctgc cctggcgcca gatcttgatc gttcgcgccg acggccatgc
     6601 ccggcgtgtt gcctgtgttg accgtcgaca cccgccctca ttatacaggt gatcgctaga
     6661 agctaccggt tacaccgacg acagcggagt cagaggtggg cgtatcgagc acaagcagcg
     6721 cggtcccggt tgcgccggcg gcgattgccg cgccaccgag gatccaggtc cacatcacga
     6781 agcgatctcg cgaacgaacg gtgcgctcga gctcgtccgc cacgctgacg ggctgattgc
     6841 ccatctcgat gctcgcttgc agatcttcag cgtggctgtt gcgcgaccgc gcgaagagcg
     6901 ccgtcgtgat cgcacctgca gccgccagtc ctgcaccgcc gtagacccac ggcactgcct
     6961 tgcgctggcc ggtgcgacgc agctcggcgt cgatgcgcag ctcttgaccg cggcccacgg
     7021 tgacttcctg cgcgaatggc tcgcggccgc ggcggagcac cgagagcaga tgcttgccgt
     7081 gcggaacctc gagtgcggcg gccggcgcgg tagcgagcgc gcgtccgtcg acgaggatct
     7141 tcgcgtcgtc gtc
//
LOCUS       DATLBZ010000522         7352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2920712, whole genome shotgun sequence.
ACCESSION   DATLBZ010000522 DATLBZ010000000
VERSION     DATLBZ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2920712"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VMZ53_26120"
     CDS             <1..774
                     /locus_tag="VMZ53_26120"
                     /inference="COORDINATES: protein motif:HMM:NF019429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HUS32015.1"
                     /translation="ASGTASRPTTGQRGTSRPTTGQRARSGTASRPSTGRTLVFVGPT
                     LSADEVRERLPDADVRPPAAVGDILRAAHESGVRRIALIDGYFERMAAVWHKEILIAI
                     ERGIEVWGAASMGALRAAELEAFGMRGVGTIYGWFKQGVITADDEVAVAHMPAEYRYK
                     AVSDALVNIRYTLAVAADEGKLAPKVAAEILAKAKATFYRERVWSKLCSTKLALVDLK
                     ADDARRLLAKLRGAGKQPRRKIRVPRTWALRQLERLMLG"
     gene            913..4512
                     /locus_tag="VMZ53_26125"
     CDS             913..4512
                     /locus_tag="VMZ53_26125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS32016.1"
                     /translation="MDGRFEVRGTLGTGGAGVVYRAFDKQLQREVALKQLRTASGRDL
                     YRFKREFRALADIVHPNLVTLHELHANGDEWYFTMELVEGVSFIDWVRPGRVNGPTRT
                     RQEIIASPVNEVRLRGALVQLIDALIALHKAGKLHRDLKPSNVLVTQQGRLALLDFGL
                     VSNLAEDNPEKLAVGTPVYMSPEQASDQSLTEASDWYSVGAMLYEALTGRRPFEGESE
                     QVMTRKQTEVPLDPRQLAIGTPVDLARLSMLLLQPSAAARPTGFAILDQLGATPSART
                     RDMVRTAAPATFVGRQRELDEMRRALTDARRRGVTVMVKGASGMGKTACVRKFLRALG
                     DSAYVLEGRCFEREQVPFKMLDGIVDMLTGVVLALPPEHVDGLAPRELASLMRLFPVL
                     KRIKRFSDLAQASSAPADPSELRRRGFQALRSVLAKLARLRPLVLFVDDAHWGDIDSV
                     VILSELIHQPDASVLVIVAHRPEDYLGVVAKLKNPQGGARRGDVRELEIGALGDADAL
                     TLVSYLATDAKRTEEVVKAGAGNPLVLSELARAPQLAVGAKVADLVKQRAGKLSAEAQ
                     AMLAVSSVAAKPIPVDIAAHAAGVVGGHDEASQLSVERLATLRRVNGQMILQPAHDHV
                     RVAVVNALDVESRAGWHEALARAYEDAGANGGEVDSLAVVEHWLAAGHPANAAHHAIA
                     AAARAEDALAFRRAAELYDIALTYGPWDAAGQRDIMKKKAEALACAGQLDEAATFYGH
                     AAQLLPDDEEGIDCERLRIEALLRRGRIDEALPAAERLLSRMGIRSSLGTSRTRLATQ
                     WMQQKLRGLDYTERDAADCKPEDLLRIDVLHSVVSGLAFADPALGRVLQAELMRVALE
                     VGEPVRVCFALAQEITYSAAAGSRNANAVAAVGTRLKSIALRLGYPHIVGLADTALGI
                     AAYMSGQWRVARQHLESGLAVLRDHGAGVRWEIDIGETYWLASLYYLGEWREMMRQGQ
                     PILRDAIERGDIVSQLGVRTGRSNLAWLIAGRPDEARAQLAAAEASLPEGFHLPHVLA
                     IQAACNIDIYADAPNAAGGRLEEAWPHFERIGALRMQHLRVELLALRARIALADSTRG
                     FDDRMRLARSIADELIKEGAPWAIGIGLLLRASTQAARGDDDGALMTLVAAENHLVET
                     EMLGWLHVARLRRGRLEGGPGGAARAEAARDLLKDAGAQNPDQIAALLVPWTS"
     gene            4604..5464
                     /locus_tag="VMZ53_26130"
     CDS             4604..5464
                     /locus_tag="VMZ53_26130"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUS32017.1"
                     /translation="MSDIAQHVAVSERINIEDLDWDLARKHGLTPLEVENLKFFVDIE
                     SQTVYYFLEVAKLQVARDPELLTFLTMWNYEEFFHSQMIIRLLQECGVKLESAQDRTT
                     NVRKNARLKAKIEDFAQTMMAKVTPKQFVALWMFWGALQECLTMQAYEEVIRNTKNPV
                     LVEMFKRIAKQERRHFAYYFGQARERLTDNKLNQRYCRFIVQHFYSPVGSGVKTEAEG
                     REMMARLFPGDRMMEVMSYIERRMAQLPGMQGLDACSRWVEKIQPMLPVELRSTGKVR
                     RPAQSDTAAA"
     gene            complement(5564..6313)
                     /locus_tag="VMZ53_26135"
     CDS             complement(5564..6313)
                     /locus_tag="VMZ53_26135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32018.1"
                     /translation="MLKPAVTVSLFLVPLLATSASYADTITTEADTLVVVTPNAPIVV
                     NQGAPQSVAPTIMAPGAQEMPPAPPTAPVAVNGAPQNEDWNNVNHINGMPVKVGEKQD
                     YLLSVKKNNISANPFGLFFGLYDISVARSLSRNVAASFAFTGTEEFYQLSATLPLYFR
                     KTFSGPFLEGGLLIRSEREYDYAYDCYDCSTYEMSSTWSGPQMMFGWHWTFDSGLNVA
                     FAGGVAKRMTNQHDYGDNVEGNAYFRVGYAF"
     gene            complement(6522..7043)
                     /locus_tag="VMZ53_26140"
     CDS             complement(6522..7043)
                     /locus_tag="VMZ53_26140"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HUS32019.1"
                     /translation="MLSQGLSTVTAAHDLDRDQLLALLDAAPRGRIGVTIHHHIPTFH
                     TEHCVYAHLLSTGRDFKTCGRPCEKHQVALRDRVGLVHPVIVDVGCRNTVFNAQAQSA
                     ASLVPELLARGVQRFRIELVRESAEEAKRLYAAYSQLVAGAIAPADAVRAAAVHEQFG
                     VTRGTMRTLTVLR"
     gene            7089..>7352
                     /locus_tag="VMZ53_26145"
     CDS             7089..>7352
                     /locus_tag="VMZ53_26145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32020.1"
                     /translation="MQLERARPALIAARLIAVPLITVPLITMPLITMPLIAEPLITVP
                     LIAETLITVPLIAETFIAETLIAVCPRGHKLLAAFVCRRGRERL"
BASE COUNT         1259 a         2343 c         2524 g         1226 t
ORIGIN      
        1 gcgagcggaa cggcgtcgcg tccgaccacc ggccagcgcg gcacgtcgcg tccgaccacc
       61 ggccagcgcg cgcgcagcgg aacggcatcg cgtccgagca ccggccgcac actcgtgttt
      121 gtcgggccga cgctgagcgc ggacgaggtt cgcgaacggc tgccggacgc tgatgtccgg
      181 ccgccggctg cggtcggcga tattttgcgc gcagcgcacg agagcggtgt tcggcgcatc
      241 gccctcatcg atggctactt cgagcgcatg gccgcggtgt ggcacaagga gatcctcatc
      301 gcgatcgagc gcggcatcga agtgtggggc gcggcgagca tgggcgcgtt gcgcgcggcg
      361 gagctcgagg cgttcgggat gcgcggcgtc ggtacgatct atggctggtt caagcaaggc
      421 gtgatcaccg ctgacgacga ggtggccgtc gcgcatatgc ccgcggagta tcgctacaag
      481 gcggtgagcg atgcgctcgt caacatccgt tacacactcg ccgtcgctgc tgacgaaggc
      541 aagctggcgc cgaaggtcgc tgcggagatt ctcgccaagg cgaaagcgac gttctatcgc
      601 gagcgcgtgt ggagcaagct ctgcagcacg aagctcgcgc tcgtcgatct caaagccgat
      661 gatgcgcggc ggttgctcgc gaagttacgc ggcgccggca agcagccgcg acggaagatt
      721 cgcgtgcctc gcacgtgggc actgcgccag ctcgagcgct tgatgctcgg gtagagcgaa
      781 gcggcggagc gttggccagc gacggcccga agcggcggag cgttggccag cgacagcggc
      841 ggagcgttgg ccagcgacag cggcggtagc gttggccagc gacggcggcg gttgctgtag
      901 gatggcgtga cgatggatgg ccgctttgaa gtacgcggca cgctcggcac gggaggtgcg
      961 ggcgtcgtct atcgcgcgtt cgataagcag ctgcagcgcg aggtcgcgtt gaagcagctg
     1021 cgcactgcat cgggccgcga cctctatcgc ttcaagcgcg agtttcgcgc gctcgccgac
     1081 atcgtgcatc cgaatctcgt cacgctgcac gagctgcatg cgaacggcga cgagtggtat
     1141 ttcacgatgg agctcgtcga gggcgtgtcg ttcatcgact gggtgaggcc gggcagggtg
     1201 aacggcccga cgaggacgcg ccaggagatc atcgcgtcgc cggtcaatga agtgcggttg
     1261 cgcggcgcgc tcgtgcaact gatcgatgcg ctgatcgcgt tgcacaaggc gggcaagctg
     1321 catcgcgact tgaagccgtc gaacgtgctc gtcacgcagc agggcaggct cgcgctcctc
     1381 gattttgggc tcgtctcgaa cttggccgag gacaatcccg agaagctcgc ggtcggcacg
     1441 cccgtgtata tgtcgcccga gcaggcgagc gatcagtcgc tcaccgaggc cagcgattgg
     1501 tactccgtcg gcgcgatgct ctacgaggcg ttgaccggac gccgaccgtt cgagggcgag
     1561 tccgagcagg tgatgacgcg caagcagacc gaggtgcccc tcgatccgcg gcagctcgcg
     1621 atcgggacgc ccgtggatct cgcgcggctc agcatgctgc ttctgcagcc gagcgcggca
     1681 gcgcggccga cgggctttgc gatcctggat cagctgggcg ccacgccgtc ggcacgcacg
     1741 cgcgacatgg tgcgcacggc tgcgcccgca acgtttgttg gacggcagcg cgagctcgac
     1801 gagatgcgcc gcgcgctgac cgatgcacga cgccgcggcg tgaccgtgat ggtgaagggc
     1861 gcgtcgggga tggggaagac ggcgtgcgtc aggaagttcc tgcgcgcgct cggcgacagc
     1921 gcgtacgtgc tcgagggtcg ctgcttcgag cgcgagcaag tgccgttcaa gatgctcgat
     1981 ggcatcgtcg acatgctgac gggcgtcgtg ttggcgctgc cgccggagca tgtcgatggg
     2041 ctcgcgccgc gcgagctcgc gtcgctcatg cggctcttcc cggtgctcaa gcgcatcaaa
     2101 cgatttagcg atctggcgca agcgagcagc gcgccggcgg atccgtcgga gctacggcgg
     2161 cgcggctttc aggcgctgcg cagcgtgctc gccaagctcg cgcggttacg gccgctcgtg
     2221 ttgttcgtcg acgatgcgca ctggggtgac atcgacagcg tcgtgatcct cagcgagctg
     2281 atccatcagc ccgacgcgtc ggtgctcgtg atcgtggcgc atcggccaga ggattatctc
     2341 ggcgtcgtcg caaagctgaa gaatccgcag ggcggcgcgc gacgcggcga tgtgcgcgag
     2401 ctcgagatcg gtgcactagg cgatgccgac gcgttgacgc tcgtcagcta tctcgcgacc
     2461 gatgcaaagc gcacagaaga ggtcgtgaaa gccggcgccg gcaatccgct cgtgttgagc
     2521 gagctcgcac gcgctcctca gctcgcggtc ggtgcgaagg tcgcggacct cgtgaagcag
     2581 cgcgcgggga agctgtcggc cgaagcgcaa gcgatgctcg cggtgtcgag cgttgcggcg
     2641 aagccgattc ccgtcgacat tgcggcgcac gctgcaggtg tggtcggcgg ccacgacgag
     2701 gcgtcgcagc tgtcggtcga gcgactcgcc acgcttcgcc gcgtgaacgg gcagatgatc
     2761 ctgcagcccg cgcatgacca cgtgcgtgtg gcggtcgtca acgcgctcga tgtcgagtcg
     2821 cgtgccggtt ggcacgaagc gctcgcgcgc gcgtatgaag acgccggagc aaatggcggc
     2881 gaggtcgatt cgcttgccgt cgtcgagcat tggctcgcgg caggccatcc cgcgaacgcc
     2941 gcgcatcatg cgattgcggc cgctgcacgc gccgaggatg cactcgcgtt tcggcgagcc
     3001 gccgagctct acgatatcgc gctcacgtat gggccgtggg atgccgccgg ccagcgcgac
     3061 atcatgaaga agaaggccga ggcgctcgcc tgcgcgggtc agctcgacga agcggccacg
     3121 ttctacggcc atgccgcgca gctgttgccc gacgacgaag agggtatcga ctgcgagcgt
     3181 ctgcggatcg aagcgctgct ccggcgcggt cgcatcgacg aggcgctgcc ggcggccgag
     3241 cgtttgctat cgcggatggg cattcgcagc tcgctcggga cgtcgcgcac gcgcctcgcg
     3301 acgcagtgga tgcagcagaa gttgcgcggc ctcgattaca ccgagcgcga tgcggcggac
     3361 tgcaagccgg aggatctgct gcgcatcgac gtgttgcatt cggtcgtcag cgggctcgcg
     3421 ttcgcagatc cggcgctagg tcgcgtgctg caagcggagc tgatgcgcgt ggcgctcgaa
     3481 gtcggcgagc cggtgcgcgt ctgcttcgcc ctcgcgcagg agatcacgta ttcggccgct
     3541 gcgggctcgc gcaacgccaa cgccgtcgct gccgtcggca cgcgcctcaa gtcgatcgcg
     3601 cttcggctcg ggtatccgca catcgtcggg cttgcggaca ccgcgctcgg catcgcggcg
     3661 tatatgagcg ggcagtggcg cgtcgcgcgt cagcatctcg agagcggcct cgccgtgctc
     3721 cgcgatcatg gtgcgggcgt gcgctgggag atcgatatcg gcgagacgta ctggctcgcg
     3781 tcgctctact acctcggcga gtggcgcgag atgatgcgcc aaggccagcc gatcttgcgc
     3841 gacgccatcg agcgcggcga catcgtcagc cagctcggcg tgcgcaccgg ccgcagcaac
     3901 ctcgcgtggc tcatcgccgg gcgtcccgac gaagctcgcg cgcagctcgc tgccgccgaa
     3961 gcatcgctgc ccgaaggttt tcacctgccg cacgtcctcg cgatccaagc cgcgtgcaac
     4021 atcgacatct acgcggacgc tcccaacgcc gcaggcgggc gcctcgaaga agcgtggccg
     4081 catttcgagc gaatcggcgc gctgcgcatg cagcaccttc gcgtcgagct cctcgcgttg
     4141 cgcgcgcgga tcgcactcgc cgactcgacg cgcggcttcg acgaccgcat gcgtctcgcg
     4201 cgatcgatcg cggacgagct gatcaaagag ggcgcgccat gggcaatcgg gatcggtttg
     4261 ctcctgcgag cttctacgca agccgcacgc ggagatgacg acggcgcgct catgacgctg
     4321 gtcgccgcgg aaaatcacct ggtcgaaacc gagatgctag gttggttgca tgtcgcgcgg
     4381 ttacggcgcg gaaggctcga aggaggcccc ggcggcgccg ctcgcgcgga ggctgcgcgt
     4441 gatttgctga aggatgcagg tgcgcaaaat cctgatcaaa tcgcagcact tctcgtgcct
     4501 tggacttcgt gactcgcgcg ctgaattcgg tatatcgtgt tcgggcgtaa tgccgacgta
     4561 gacgatccgt gggggatggt cagtcgtaca gcggacagag ggtatgtcag acatcgcaca
     4621 acacgtcgcc gtttccgaac gcatcaacat cgaagacctc gattgggatc tcgctcgcaa
     4681 gcacgggttg accccgctcg aggtcgagaa cctaaagttc ttcgtcgata tcgagagtca
     4741 aacggtctac tacttcctgg aggtcgccaa gctccaggtc gcgcgtgatc cagagctgct
     4801 cacgttcctc acgatgtgga actacgagga gttttttcac tcgcagatga tcattcgttt
     4861 gctgcaggag tgcggcgtca agctcgagag cgcgcaagac cgcacgacga acgttcgcaa
     4921 gaacgcgcgg ctcaaggcca agatcgaaga cttcgcgcag acgatgatgg cgaaggtgac
     4981 gccgaagcaa ttcgtcgcac tgtggatgtt ctggggcgcg ctgcaagagt gtctgacgat
     5041 gcaggcgtac gaagaggtca tccgcaacac gaagaacccc gtgctcgtcg agatgttcaa
     5101 gcggatcgcg aagcaagagc gccgtcactt cgcgtactac ttcggccaag cgcgcgagcg
     5161 tctcaccgac aacaagctca accagcgcta ctgccgcttc atcgtccagc acttctactc
     5221 gccggtcggc tccggcgtga agaccgaggc ggagggccgc gagatgatgg cccgtctgtt
     5281 cccgggcgat cgcatgatgg aagtcatgag ctacatcgag cggcgcatgg cgcagctgcc
     5341 cggcatgcag ggcctcgacg cctgctcgcg gtgggtcgag aagatccagc cgatgctgcc
     5401 cgtcgagctc cgcagcaccg gcaaggtccg tcgccccgcg cagagcgaca ccgcagccgc
     5461 gtaacaccgg catccgaaac ggcgaagagc acgctcccgg caagggcgtg ctttcgtcgt
     5521 ttcggcgtca caatgctcgc gcgacgtcgt tcggtgccgc tcgttagaac gcgtagccga
     5581 cgcggaagta cgcgttgcct tcgacgttgt cgccgtagtc gtgctggttc gtcatgcgct
     5641 tcgcgacgcc gcccgcgaat gcgacgttca agcccgagtc gaacgtccag tgccagccga
     5701 acatcatctg cggaccgctc cacgtcgacg acatctcgta cgtcgagcag tcgtagcagt
     5761 cgtacgcgta atcgtactcg cgctcgctac ggatcaggag gccgccctcg aggaacgggc
     5821 cgctgaacgt cttgcggaag tacagcggca gcgtcgccga cagctgataa aactcttcgg
     5881 tacccgtgaa cgcgaagctc gcagccacat tgcggctgag cgagcgcgcg accgagatgt
     5941 catagaggcc gaagaacagg ccgaacgggt tggccgagat gttgttcttc ttcaccgaga
     6001 gcaagtagtc ctgcttctcg ccgactttca cgggcatgcc gttgatgtgg ttgacgttgt
     6061 tccagtcctc gttctgcggc gcgccattca cggccacagg cgccgtcgga ggagccggcg
     6121 gcatctcctg cgcgccgggc gccatgatgg tcggcgcaac tgactgcggc gcgccctggt
     6181 tcacgacgat gggggcgttc ggcgtgacca cgacaagcgt gtccgcttcg gtcgtgatgg
     6241 tgtcggcata cgacgccgag gtggcaagga gcggaacgag gaagagcgag acggtgacgg
     6301 ccggcttgag catggcggcc tctagagcac cccggatgcc aggtcacacg taacgtttga
     6361 agtgcccgtc tagtctcgaa tttttgctgc agaacgcggc aaacccgtgt cagaggccga
     6421 ccgccagttc gacgaaagtc gagagaatcc tgacacgcct gtcgcgatcg aggccggcgt
     6481 ctaccacttc gtgaagatcg cgtgatcgcg cgcaggtacc gtcaacgcag gaccgtcaag
     6541 gtccgcatgg tgccgcgcgt gacgccgaac tgctcgtgca ccgccgctgc ccgcaccgcg
     6601 tccgcgggcg cgatcgcccc ggcaacgagc tgcgagtacg cggcgtagag ccgtttcgct
     6661 tcctcggccg actcccgaac gagctcgatt cgaaaccgct gaacgccgcg cgcgagcagc
     6721 tcgggcacga ggctcgcggc gctctgcgcc tgtgcattga agaccgtatt gcgacaccca
     6781 acgtcgacga tcacgggatg cacgaggccc acgcgatcgc gcagcgcgac ctgatgcttc
     6841 tcgcacggcc ggccgcacgt cttgaagtcg cgaccggtcg acagcaaatg cgcatacaca
     6901 caatgctcgg tgtgaaacgt cggaatgtgg tggtggatcg tcacgccgat gcgtccacgc
     6961 ggcgcggcgt cgagcaacgc gagcagctga tcgcgatcca aatcgtgcgc cgccgtaacg
     7021 gtcgagagcc cctggctcag cacccacgcc gctgtgatcg agttcgtgac gttgagcgaa
     7081 aaatcgccgt gcagctcgag cgcgcgcgac ccgcgctcat cgccgcgcgg ctcatcgccg
     7141 tgccgctcat caccgtgccg ctcatcacca tgccgctcat caccatgccg ctcatcgccg
     7201 agccgctcat cacggtgccg ctcatcgccg agacgctcat cacggtgccg ctcatcgccg
     7261 agacgttcat cgccgagacg ctcatcgccg tgtgcccacg tgggcacaag ctccttgctg
     7321 cgttcgtgtg tcgacgtggg cgcgagcgcc tt
//
LOCUS       DATLBZ010000523         3623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2920990, whole genome shotgun sequence.
ACCESSION   DATLBZ010000523 DATLBZ010000000
VERSION     DATLBZ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2920990"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VMZ53_26150"
     CDS             <1..183
                     /locus_tag="VMZ53_26150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32021.1"
                     /translation="EALAQATQRLANADRALKGDITLTTQTTEFTGPQLKALGRELAE
                     RVILEKIVDGIVELAS"
     gene            185..988
                     /locus_tag="VMZ53_26155"
     CDS             185..988
                     /locus_tag="VMZ53_26155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32022.1"
                     /translation="MCCFSIPTPKSFWQRLRAPKIHVSATQIFGRIVAPGVQGLAYSM
                     NLESAREVAMILPIPVRPGTGESALRFLNLEKHPRMFTDLDALFVDISNLPRKGGLSV
                     AARPQTLVVHDVGSFIATYVPTKLDFDRVDRQFWLPRILLDSVPAYSDYGFAVFQLKA
                     GKHTVHPMAFTFPSRELDRLYFPTVHLHDGQFRRDAEFDHALYYQHPRVREQGGTFEG
                     DPTSYLMPSSDYEGLVDKSRPIVKRTVRGKQPNQDIWVKTGRPITVNSV"
     gene            complement(985..2277)
                     /locus_tag="VMZ53_26160"
     CDS             complement(985..2277)
                     /locus_tag="VMZ53_26160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32023.1"
                     /translation="MYRYGDGTPFPLEENFIETLTSAVEACTNAFMPLTELDGRRERA
                     RAGRQEADRELSKLAELEKAVTSALSPYMVPDKQSPQSQSVAQKTLAAAKTNIQQARA
                     LVDGRVQALEAQAGAGTAADAVLHALRPFFDHFQLPSTKWIMSWDVRGPEPRADAVAT
                     AGRLSASFSLQLETLRGPIRVGDLADGVIVHMMKKGLLGKAKPAPVELAKYVMVSYER
                     TASEHVITLKEKPDKASQGLRFMVSETGASWQSMGVSGDAETDPNPLDFEDVDGIRRL
                     GEGASKALKDLVNHRVLTDLTLGNQELTKLAEPRIVPMEVLQQLTPLARTIRERSRVS
                     GELVLKRDVAGGRREELFINRAQLAQHFSKLPMEYRKPFEDMGISSEDTQPAISIGSP
                     MLRPPAAPSGGKVPPAPPGSSTVKIDEDQITKVDAKKD"
     gene            complement(2299..3546)
                     /locus_tag="VMZ53_26165"
     CDS             complement(2299..3546)
                     /locus_tag="VMZ53_26165"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HUS32024.1"
                     /translation="MQLRIAIALVLLTSSAHADDRAEVSTSVFAESRQGDQGGLLVIH
                     PQADLDSDIGKHVSLGLGYSADAVSGATAAVYQVDATSGATKFSDVRHEGQLSLGFHG
                     RRSSLTFSTTVGTERDYLSRQFGGTASIDLPGRNTSVSLAYSHSYDEVCDKENADLMP
                     LERRALIGDDPCTKTGLLFGKDTAGMTTWRPLSIDTAAVTLTQNVSPTMNLQLALYGQ
                     ILEGFQSNPYRRVRIGSNSPQEHIPDTRDRWSITARMNRFLPKLHGAAHFSARFYDDT
                     WHVLGGDLELAYSQYIGNALLARFRLRAYQQTAAAFFKDAFFYETESTAGEYFSGDRE
                     LSPVRSALAGAKLTLISVGEDKPVWGLFDKLQFNLKGDLLLLFPVAADDVAANPMGID
                     KQFLNGSSLLDAFVLQLGLLGNY"
BASE COUNT          602 a         1172 c         1188 g          661 t
ORIGIN      
        1 gaggcgctcg cgcaagcgac gcagcgtctc gcgaacgccg atcgcgcgct caagggcgac
       61 atcacgctga ccacgcagac cacagagttc accgggccgc agctcaaggc gctcggacgc
      121 gagctcgcgg agcgcgtgat cctcgagaag atcgtcgacg gcatcgtcga gctcgccagc
      181 tgagatgtgc tgcttctcga tcccgacgcc caagtcgttc tggcagcggc tccgtgcacc
      241 caagattcac gtctcggcga cgcagatctt cgggcgcatc gtcgcgccgg gtgttcaggg
      301 cctcgcgtac agcatgaacc tcgagagtgc acgcgaggtc gcgatgatct tgccgatacc
      361 ggttcggccc ggaacaggtg agtcggcgct tcgcttcctg aacctcgaga agcacccgcg
      421 catgttcacc gacctcgacg cgctgttcgt cgacatctcg aacctgccgc gcaaaggtgg
      481 gctctcggtt gccgcgcgtc cccaaacgct cgtcgttcac gacgtgggct cgttcatcgc
      541 gacgtacgta ccgacgaagc tcgatttcga tcgcgtcgac cgccagttct ggctgccgcg
      601 gatcctgctc gattcggtgc ccgcgtacag cgactacggc tttgcggtct ttcagctcaa
      661 ggccggcaag cacacggttc atccgatggc gttcaccttt ccgtcgcgcg agctcgatcg
      721 cctctacttc ccgacggtgc atctgcacga cggccagttt cgtcgcgacg ccgagttcga
      781 tcacgcgctg tactatcaac acccgcgcgt gcgcgagcag ggcggcacgt tcgaaggcga
      841 tccgacgtcg tatctgatgc cgtcgagcga ttacgaaggg ctcgtcgaca agtcgcggcc
      901 gatcgtgaag cgcacggtgc gcggcaagca gccgaatcag gacatctggg tcaaaaccgg
      961 gcgaccgatc acggtcaact cggtctaatc cttcttcgcg tcgaccttcg tgatctgatc
     1021 ttcgtcgatc ttcacggtcg acgagccggg cggggcaggc ggaaccttgc cacccgacgg
     1081 agcggcaggc ggccgcagca tcggagagcc gatcgagatc gcgggctgcg tatcctcgct
     1141 cgagataccc atgtcctcga acggcttgcg gtactccatc ggcagcttcg agaaatgctg
     1201 cgcgagctgc gcgcggttga tgaagagctc ttcgcggcgg ccgccggcga catcgcgctt
     1261 gagcacgagc tcgccagaca cgcggctgcg ttcacggatc gtgcgcgcga gcggcgtgag
     1321 ctgctgcagc acttccatcg ggacgatgcg cggctcggcc agcttcgtca gctcttgatt
     1381 gccgagcgtc agatccgtca gcacgcggtg gttgacgaga tccttcagcg ccttgctcgc
     1441 gccttcgccg aggcgacgaa tgccgtcgac atcttcgaaa tcgagcgggt tcggatcggt
     1501 ctccgcgtcg ccgctgacgc ccatcgactg ccagctcgca ccggtctcgc tgaccatgaa
     1561 gcgaaggcct tgcgacgcct tgtccggttt ctctttgagc gtgatgacgt gctcgctcgc
     1621 cgtgcgctcg tacgagacca tcacgtactt cgccagctcc accggcgctg gcttggcctt
     1681 gccgagcagg cccttcttca tcatgtgaac gatcacgccg tcggcgagat caccgacccg
     1741 aatcggtccg cgcagggttt cgagttggag cgagaacgaa gcggagaggc gaccggcggt
     1801 agctaccgcg tcggcgcgag gctcgggacc gcgaacgtcc cacgacatga tccacttcgt
     1861 gctcggaagc tggaaatggt cgaagaacgg acgcagcgcg tgcagcactg catcggcggc
     1921 agttccggcg cccgcttgcg cttcgagcgc ttgcacgcgt ccgtcgacga gagcgcgcgc
     1981 ctgctggatg ttggtcttcg ccgcggcgag cgtcttttgc gcgaccgact gtgactgcgg
     2041 gctctgcttg tccggaacca tgtagggcga caacgcactc gtgaccgctt tctcgagctc
     2101 cgcgagcttg ctcagctctc ggtcggcctc ctgccgccca gctcgtgcgc gttcacgtcg
     2161 accatcgagc tccgtcagtg gcatgaaggc gttcgtgcac gcctccaccg cagacgtcag
     2221 ggtctcgatg aagttttcct cgagaggaaa tggcgtgccg tccccatacc gatacatgcc
     2281 gtccggagta tacgcgcgct agtagttgcc gagcaaaccg agctgcagca cgaacgcatc
     2341 taggaggctc gagccgttca ggaactgctt atcgatgccc atcggatttg cggctacgtc
     2401 gtcggcggcg accgggaata gcagcaggag gtcgcccttc aggttgaact ggagcttgtc
     2461 gaacaggccc cagaccggct tgtcctcgcc gacgctgatc agcgtcaatt tggcccctgc
     2521 gagggcgctg cgaacgggcg acagctcgcg gtccccgctg aagtactcgc cggcggtcga
     2581 ctcggtctcg tagaaaaacg catctttgaa gaacgccgcg gcggtctgct gataggcgcg
     2641 caggcggaag cgcgcgagca gcgcgttgcc gatgtactgc gagtacgcga gctcgagatc
     2701 cccgccgagg acatgccacg tgtcgtcata gaagcgcgcg gagaaatgcg cggcgccgtg
     2761 cagcttcggc aggaagcgat tcatgcgtgc ggtgatcgac cagcgatcgc gcgtgtcggg
     2821 gatgtgctcc tgcggagagt tcgagccgat gcgaacccgg cgatacggat tgctctggaa
     2881 gccctcgagg atttggccat acagggcgag ctgcaggttc atcgtcggcg agacgttctg
     2941 cgtgagcgtg acggcggcgg tatcgatcga gagcggtcgc cacgtcgtca tgcccgcggt
     3001 gtccttgccg aacagcaggc cggtcttcgt gcatggatcg tcgccgatca atgcgcgccg
     3061 ctcgagcggc atgaggtcgg cgttctcctt gtcgcagacc tcgtcgtagc tgtggctgta
     3121 cgcgagcgag acgctcgtgt tgcgaccggg cagatcgatc gacgccgtgc cgccgaattg
     3181 ccgcgagagg tagtcgcgct cggtgccgac cgtcgtcgag aacgtgaggc tcgagcggcg
     3241 gccgtggaag ccgagcgaga gctggccttc gtggcggacg tcggagaact tcgtcgcgcc
     3301 gctcgtcgca tcgacttggt acacggcggc cgtcgcgccg ctcaccgcgt cggcggagta
     3361 accgaggccg agggacacgt gcttgccgat atcgctgtcg agatcggcct gcggatggat
     3421 cacgaggagg ccgccctggt cgccttgacg cgattccgcg aacaccgagg tcgagacctc
     3481 tgcgcgatcg tccgcgtgcg cgctgctcgt cagcagaacg agcgcgatgg cgatcctcag
     3541 ttgcaaccgc agcctccgcc gccggcgccg tggccgcccg atgcgccttc gcggttcgag
     3601 agcacgtgat cttcggcctc ggt
//
LOCUS       DATLBZ010000524         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4385472, whole genome shotgun sequence.
ACCESSION   DATLBZ010000524 DATLBZ010000000
VERSION     DATLBZ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4385472"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="gltD"
                     /locus_tag="VMZ53_26170"
     CDS             complement(<1..478)
                     /gene="gltD"
                     /locus_tag="VMZ53_26170"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831769.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HUS32025.1"
                     /translation="MGKPTGFLEWDRAAPHRRPVALRVLDFKEVEELDPPDTSRQQAG
                     RCMDCGVPFCTQGCPLGNPIPDFAHAIWTDRWHDAHKQLAVTNEFPEFTGRLCPAPCE
                     GACVLAINSDPVTIEHLERSISERAFAEGWVQPRPPVTRTNKRVAIVGSGPAGLAAA"
     gene            complement(478..>2137)
                     /locus_tag="VMZ53_26175"
     CDS             complement(478..>2137)
                     /locus_tag="VMZ53_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HUS32026.1"
                     /translation="GGQLPGNKVDERIAKARNSTPGVSLISPPPHHDIYSIEDLAQLV
                     YDLTAINPRARVSVKLVSEVGIGAIAAGVAKARASCVVIAGYEGGTGASPLSSLKRAG
                     LPWELGLAETQQVLVENRLRDRIRVQVDGGLRTSRDIIVAALLGGEEYGMATASLIAS
                     GCVMLRKCHLNTCSVGIATQDPELRKKFTGTPEDVVAYFTFVAEGVRRRLAELGARSL
                     DEIIGRVDFLKVRKRSLKKHAKTRDLDLSAIVSPPITHRKLPLRFSAAQPWPLEDHID
                     HAIIAAGALEKHPMSVELPIDNSKRAAGTLLSGDIARRYGARGLPDGSIHVKLTGSAG
                     QSFGAFLAAGVTLELAGDANDYIGKGLSGGRIVVMPPQGSRFVAEDNVIIGNVALYGA
                     TAGDLFANGLAGERFAVRNSGSRAVVEGCGDHGCEYMTGGVVVVLGPVGRNFAAGMSG
                     GTAFVFDEKQTFRSRCNLDMVELEPLVEESDLWLVHSLIEDHVRFTGSPRGKKLLDTW
                     EHILARFVKVMPTEYKRVLEKRRTANRSRPVGIPTPAAVVGGGS"
BASE COUNT          327 a          679 c          777 g          354 t
ORIGIN      
        1 cggccgcggc gaggcccgca ggaccggagc cgacgatcgc cacgcgcttg ttcgtgcgtg
       61 tgactggggg gcgaggttgc acccagcctt ccgcgaacgc gcgctcgctg atcgagcgtt
      121 cgaggtgctc gatcgtgact ggatcggagt tgatcgcgag gacgcacgcg ccttcgcatg
      181 gggcggggca gaggcggccc gtgaattcgg ggaactcgtt cgtgacagcg agctgcttgt
      241 gggcgtcatg ccagcgatcc gtccagatcg cgtgtgcgaa gtccgggatc gggttgccga
      301 gcgggcagcc ttgcgtgcag aacggcacgc cgcagtccat gcagcgacct gcttgttggc
      361 gcgaggtgtc gggcgggtcg agctcttcga cctccttgaa gtcgaggacg cgtagggcga
      421 cgggccggcg gtgcggcgcg gcgcgatccc attcgagaaa gccggtgggc ttgcccatta
      481 ggagcctcct ccgacgacag cggccggcgt gggaatcccg acaggccgcg agcgatttgc
      541 ggtgcggcgt ttctcgagca cgcgcttgta ctccgtcggc atcactttga cgaagcgcgc
      601 gaggatgtgc tcccacgtgt cgagcagctt cttgccgcgc gggctccccg tgaagcgcac
      661 gtggtcttcg atcagcgagt ggacgagcca caggtcggat tcctcgacga gcggttcgag
      721 ctcgaccatg tcgaggttgc agcgcgagcg gaacgtctgc ttctcgtcga agacgaaggc
      781 cgtgccgcct gacatgcctg cggcgaagtt gcggccgacc gggccgagca cgacgacgac
      841 gccgcccgtc atgtactcgc agccgtgatc cccgcagcct tcgacgaccg cgcgcgagcc
      901 ggagttgcgg accgcgaagc gttcgccggc gaggccgttg gcgaagaggt cacctgcggt
      961 ggcgccgtag agtgcgacat tgccgatgat gacgttgtcc tcggcgacga agcgcgagcc
     1021 ttggggcggc atcacgacga tgcggccgcc ggacaggcct ttgccgatgt agtcgttcgc
     1081 gtcgcctgcg agctcgagcg tgacgcccgc ggcgaggaac gcaccgaacg attggccggc
     1141 ggagccggtg agcttcacgt ggatcgagcc atcgggaagg ccacgtgcac cgtagcgacg
     1201 tgcgatatcg ccggagagca gcgtgcccgc ggcgcgcttg ctgttgtcga tcggcagctc
     1261 gacggacatt ggatgcttct cgagcgcacc ggccgcgatg atcgcgtggt cgatgtggtc
     1321 ttcgagcggc cacggctgcg cagcgctgaa ccgcagcggc agcttgcggt gcgtgatcgg
     1381 cggcgacacg atcgcggaga ggtcgaggtc gcgcgtcttc gcgtgcttct tgagcgagcg
     1441 cttgcggact ttgaggaagt ccacgcgacc gatgatctcg tcgagcgagc gcgcgccgag
     1501 ctccgcgagc ctgcgccgga cgccttcggc gacgaacgtg aagtacgcga cgacgtcttc
     1561 gggcgtgccc gtgaacttct tgcgcagctc gggatcctgc gtcgcgatgc cgaccgagca
     1621 cgtgttgagg tggcacttgc gcagcatcac gcagccgctc gcgatgagcg aggccgtggc
     1681 catgccgtac tcttcgccgc cgagcagcgc ggcaacgatg atgtcgcgcg acgtgcgcaa
     1741 gccgccatcg acttgcacgc ggatgcggtc gcgcaggcga ttctcgacga ggacttgctg
     1801 cgtctcggcc aggccgagct cccacggcag acccgcgcgc ttgagcgacg acaacgggga
     1861 cgcgccggtg ccgccttcgt accccgcgat cacgacgcac gatgcccgtg ccttcgcgac
     1921 accggcagcg atcgcgccga tgccgacttc actgacgagc ttcacgctga cgcgcgcgcg
     1981 cgggttgatc gccgtcagat cgtagacgag ctgcgcgaga tcttcgatcg aatagatgtc
     2041 gtggtgcggc ggcggcgaga tgagcgagac gccgggcgtc gagttgcgcg ccttcgcgat
     2101 gcgctcgtcg accttgttgc cgggcagctg gccacct
//
LOCUS       DATLBZ010000525        22842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10782885, whole genome shotgun sequence.
ACCESSION   DATLBZ010000525 DATLBZ010000000
VERSION     DATLBZ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22842
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10782885"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..540)
                     /gene="msrB"
                     /locus_tag="VMZ53_26180"
     CDS             complement(94..540)
                     /gene="msrB"
                     /locus_tag="VMZ53_26180"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HUS32027.1"
                     /translation="MPTSKSGYDVTRLADDRIQALASEFSPEERHVILHQGTERPFCG
                     LLLDNKKPGTYTCRLCGLPLFSSDSKFESGTGWPSFFKPYDPAHVTELADESHGMRRV
                     EIRCARCDAHLGHVFDDGPPPTRLRYCLNSVSMDFRENGQPLELKT"
     gene            615..1469
                     /locus_tag="VMZ53_26185"
     CDS             615..1469
                     /locus_tag="VMZ53_26185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32028.1"
                     /translation="MKGRLWGAAAGVVALGLTGCFDEQITHCPTVDCPIEMVCDGVGG
                     CAVPEQLSQCSGQTDGAACSFSTLSNVHIDGACETGVCRSREIPACLFDQFLDSRVDV
                     GMWELWLPSNEPIVVSEEPGRLAIAIAPDVGRLYNGIQSRGRYDMLGGNASVEVEPAG
                     QEIGVETTFAVDIDSTLGFEMSAYADRLHLVVHSSGGVTNSIPVDYDPVAQRFWRFRH
                     DTGAGTMEMETSPDGNNWTSQRSAALARPPTGVVVTLLAGTYIDRGAASPGVAYFHRV
                     KLTSATCP"
     gene            1493..5071
                     /locus_tag="VMZ53_26190"
     CDS             1493..5071
                     /locus_tag="VMZ53_26190"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HUS32029.1"
                     /translation="MTSFVAAFIAFGAACGSPNATTDARPPQRDAPPGSGQLGDDCAQ
                     HSDCAGFCVEAVGGIGGVCTRECNDDCPAEWTCREIEISGTAVRACVPDAPQLCLACV
                     DDAECGTGAACLTIDNQGSCATKCTVDCPAGYTCAVDPKGTHPGNYCQPITGSCTCSV
                     GMDGMSRACTNTNTIGTCFGTQLCNPLVGWSTCTALAAALEDCDGNDNDCDFLIDEDT
                     GGGQACSNTVAGIGTCAGTRQCGGSVGYVCEGQIPSAEKCNYADEDCDTRVDETFSGL
                     GTICSPGVGGCQRVGSVRCKADGTATECSVVAGTPTSELCNRVDDNCNGTTDETFPTL
                     GNSCSVGAGTCQRFGTTVCATNGTSTTCSVTAGTPTTELCNALDDNCNGTADETFTML
                     GNGCAVGTGACQRFGTTVCASNQTSTTCSATAGSPTAELCNAIDDNCNGMNDETFTTL
                     GNGCSAGIGACQRFGTTVCASNQTSTTCSVVAGSPVAELCNKIDDNCNGSADETFTTL
                     GNSCSVGIGACQRFGTTVCAGNQTSTTCSATAGTAGTELCNAVDDNCNGSVDETFTTL
                     GNACSAGFGVCQRFGTTVCASGGMTTACTATAGTNSSSETCNFLDDDCNGVVDNGFRN
                     AGTGVYDQTANCGSCGNDCAAIYTGANSAGQCSTATGSPTCVMVCSAGTSDLNASSFD
                     GCEFVLDNTTVYVSTTDSAAADDATCGLGPTGSGAGNHPCKTITFGLTRAGTLARPNV
                     RVADGTYNESVTLVSGKNVYGGYRSGTWDRHLATTSTLIQGVSSVGVHDRTIVATNVS
                     NAIFEGFVVRGSFNTKATGNSYAIYISGGASTLVIKDNVVFAGRGGAGSEGGAGTVGS
                     TGPNGTGSVNGSYDGYVAGSPPCSASRQFNNGAVFSCGGDNVSGGNGGGNKCRPATDY
                     TQFSGINGFGGQPAAGAGGGAAGGTSQGGFDGTMQTQGQNTTCFLPGPSMAGADGASG
                     MSGSNGSGVAGCSASTGTVSSGEWVNGAAPSGVVGFNGGGGGGGAAGGGGACTACGGS
                     LNRDRLGAHGGGGGAGGCGGAGGNGGTGGGGVFGIFIVGGTAPTLTNNSIQRGAGGPG
                     GDGGIGGAGGFGGRGGAGGAQVMLCAAKAGVGGNGGNGGYGSGGGGGCGGSSYGIFTS
                     GIGTPTYCTNNTVSGGSAGAGGVGGFSVGNSGANGTAGVLQGCTSI"
     gene            5071..8682
                     /locus_tag="VMZ53_26195"
     CDS             5071..8682
                     /locus_tag="VMZ53_26195"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HUS32030.1"
                     /translation="MRTTRYIVLAFALCFVGCGGSNEKTDASVSDAFGGANFGAPCRT
                     AEDCPGGMCAETPSGGVCSSTCNGACPEGFNCRVRDVGGSLESICVPQLFDYCTACNS
                     DAQCNGGVCVDLEGTKACLAECPHQGSCPTGYTCGSDPTGQHIGSFCVPLTATCSCTV
                     AEQGQVRTCTKSNVLGTCRGIETCDADNGGWVGCSALDATTESCDGSDNDCDGLIDEG
                     VGGSPCMNTVAGVGSCAGVTRCQGAAGVTCEGKTPVAETCNYADDNCDGMPDETYAGL
                     GNVCSVGVGTCQRYGVVRCNTAGTATECSVTAGSAVTELCNALDDNCNGSIDETFTTL
                     GNSCSVGVGACTRQGNLVCNGAGTGTTCSVAAGSPVTELCNGRDDNCNGSTDETFTTL
                     GQACSVGVGVCTRQGSFVCNGAQNGVACSVAAGMPGTEVCNSLDDNCNGSIDEAFPTL
                     GQACSVGVGVCTRQGSLVCNGAQNGTACSVTAGAPGTEVCNSLDDNCNGSVDEAFPTL
                     GQACSVGVGACIRQGNLVCNGAQNGTTCSVAAGSPTAELCNSIDDNCNGMTDETFTTL
                     GQACTVGVGVCARQGNMVCNGAQNGAVCSVAAGTAGTETCDTRDDDCDGNTDEGWKNQ
                     STGVYDQANACGSCANDCTVSYALPNAAGVCNLTGSPHCAMQCAANAFDLDSALANGC
                     ELLLDTSAVYVSTDSAGSADDATCGQGPQGTGSGFHPCKSITQGLTRAAALGRPRVLV
                     ANGIYPESFTLVNGRSMLGGYNPDSWLRNVATTSTYITGSNTIATSHDYAVSAINITS
                     ATVFEGFVVVGPVNSKVGGNSYAIYISGSNSNLSVINNVIASGRGGPGSNGSSGLSGS
                     TGVDGTGRSSNPTAYDTKDAAASPCTLPTRQYANGGTLSCGTNVVSGGSGGGNRCAPK
                     NDYTIFSAFTGSTGNAGVGLLGGAAGLGGEAGHDMELAVDAGGDICYISTTAGWHDYG
                     FDGKNGSSGLNGTGVAACTAAGGSVSGGHWVGGSGLGGVGGGNGGGGGGGGGGGGATC
                     VGCPSGHDELGGHGGGGGAGGCGGTGGGSSTAGGGVFAIFVVGGASAPVITGNNIQRG
                     QGGAGGRGGAAGVGGAGGFGGDGGLVASPMFCPALAGRGGNGGAGGHGSGGAGACGGV
                     SIGIYTSGLGASMNYCLAAGNNTITGGASGPGGPGGYSVVNVGPTGANGSLSACSFN"
     gene            8752..10278
                     /locus_tag="VMZ53_26200"
     CDS             8752..10278
                     /locus_tag="VMZ53_26200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32031.1"
                     /translation="MADLPEAPDEDASTNVNTPPPGRPALKRRITRPSYMVELTEGSM
                     VGEYEVQSQIGEGAMGTVFMGVHPLIGKRVAIKVLKPELCANQASIDRFVQEAQAVNK
                     IGHPNIVDVFSLGELEDGRAYFVMEFLRGEDLKVKLSHGPMPVAETCDILDGIARALE
                     AAHAKEIIHRDLKPDNVFLHQVDNGPMMVKLLDFGIAKLVRTNPGQEKTQTGNMLGTP
                     RYISPEQARGIQVDHRSDIYSLGVIAYEMLAGRPPFDGETAMDLVVKHLNEDAPPLSQ
                     FVKVPRNLEQCVMRMLAKDAKARPSLEEIRGILVDPTRRLTPVPRSTAHAMPKSPAKP
                     RWPLLLGAVVLVAAAGVATWKLVLDKGDDKPAAVASEAPVPVEKQVPEVKQPEQPVQP
                     AASAPAPVERGTLEVEVKGAKDSTIYVDGEEWGRGASVKVPLEPGTHEIKVKPPGRAA
                     IVQTTTIESGQSNKLTIVVPKEKTIVIRRGSAKTQPKETPPPPPSDDALLAPKKRKGK
                     "
     gene            10275..11075
                     /locus_tag="VMZ53_26205"
     CDS             10275..11075
                     /locus_tag="VMZ53_26205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32032.1"
                     /translation="MKSLVIVCALAGAVYAAPKEKPKKVDVAQTQADALYEKGQQSYQ
                     AGEYQEAIEHFKRAYELVKDPVYLFNIAQSYRKVADCLNAHAFYTQYLQEAPDAENKD
                     KVTQWLSELQPCVDQRHNEQEAARRAEEADRVRKADDARRAAEARKPVPVEVDRGGAL
                     RIAGIATGTVGLAVLSAGIVYSINGRSIKNDLADRCSMGCQWDSMEILDLDAQGKRAN
                     TRAKIGTIAGGIATVVGVSLYIVGRTRVETVMITPNAGGATVSAQLTF"
     gene            11089..14160
                     /locus_tag="VMZ53_26210"
     CDS             11089..14160
                     /locus_tag="VMZ53_26210"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32033.1"
                     /translation="MPGDREPSEDELARTATVGATATVTGEPGRDVPPVRDSDVGDQL
                     GRYKLQREIGRGGMGVVHAAFDPDLERRVALKVLPVTSESVEARQRLLREARAMARLA
                     HPNVVTVHEVGTAKGRDFIAMELVDGKTLEDWLSAKPRTEREIIEAYVAAGRGLAAAH
                     AAGLVHRDFKPRNVLRHRDGRIAVTDFGLVVGVEAQADAFTTTLPSGGLAALDETTTP
                     SSLSGLTKTGSVLGTPAYMAPEQWTGGTIGPPADQFAFCVALWEALAKERPYRGDTLE
                     ALKEKVIAGRRADDSKIPRKLRGVLRRGLDPDPAKRYPDMNALLAAIVRTERRPTLLF
                     ALGAAAIVAAVVVYFVAARSPTVQKTYVVGERCSAPVLAPDKVWTAGDLERLRAAQQG
                     LAADLIDADMKAWHELRARACKAQADVREPQLACLDGVLSRIDTVKQALGIPNVSPQL
                     EINELLVDPATCNTAQPPRLTRVMSGEMRGLIGRYLVSQTPGRELKASDAAALIAAAG
                     GEPCALSFAHQMAVGITPGSERADHLARAERASQECADDRLLADTAMLSSMIAGLLAE
                     NELRTGLQRSEALAARVSEPFLEAQLEQQRVSLAMISDDIDGAVAHSEKALELFRDAG
                     AVRSALTAQLDLTSWKDRRGGANDFAGRGEELATLRADIVKKLGESDRAVANIDARRA
                     DWMWQNGDLAGAEQLRRKLVQRDPAFEPHVIAGQVVDDKGPVEGAEVTVAPSMWGDSL
                     RAAETLGSLQRSTVTGADGRFSISDVETKSYLIAQHGDQRSAAVLATPNMTVMLTPTS
                     RVSGTVDLRGHAPSQVTLIISAKSQPKETPYYMRAPIGEDGTFSVDGVPRGAVLFQTS
                     VALPTARMVAGKEVNVSKPVVEGVRLEVKASARPLNVLVRSIYGAPIVKANVFLIAGK
                     QPPDGFFGKMFSGKENYTPKLALPPQPEQQTPIVRARMKSGDLLARFAQRPEGEVSVC
                     AMPMPDRLDDPQFQAAGMDLKRLEKWPVKCVPMKSDDELVEIAIPPLPRFD"
     gene            complement(14167..14856)
                     /locus_tag="VMZ53_26215"
     CDS             complement(14167..14856)
                     /locus_tag="VMZ53_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HUS32034.1"
                     /translation="MTAFVLGATGFVGREVVRQLCVRGVKAHAHVRPDSKQLAEWQTR
                     FGELGAQVDTTPWDAMALAARWRELKPAQIYICIGTTRSKAKADSIEGNIYEKVDFGL
                     TKLAVDAARGSEVSPRLVYLSSVGADPKARSAYLAWRGKAEDAVKGSGLAWVIARPSI
                     ITGERDDSRLGEKTAGVIGDGLLAVVGVFGAKSLRARYRSTTPDVLASALIRLGEAPE
                     HDRIVDGADLR"
     gene            complement(14859..15800)
                     /locus_tag="VMZ53_26220"
     CDS             complement(14859..15800)
                     /locus_tag="VMZ53_26220"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUS32035.1"
                     /translation="MAKALEYNATLAERIDVTDALTIFRVKPDQEPARPWFVPGQYCV
                     LGMNNAEKPELGSVRRSMSIASAPEDAGPTEFYIRFVSKPESENPLTHLLWKLKTGDR
                     MYMRPVAAGVFTVKDTIGEADTRLRVCVAAGTGSAPFLSMVRSELRKNPDADLSKYVL
                     LHGASYPDDLGYRDELMQMVERNGLKYWGTVSRPKEAPSWKGDTGRVEAFFESGRIDD
                     LETRVGLPAGGFTPKNVVIFICGLTGTITGTIIPLMDRGFVPDFKRIREALGVPPETK
                     DSVFYEQYDTEPVINVKDPAVVEPLKARMQAGLAKLS"
     gene            complement(15886..16485)
                     /locus_tag="VMZ53_26225"
     CDS             complement(15886..16485)
                     /locus_tag="VMZ53_26225"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HUS32036.1"
                     /translation="MRTIIRSARVLALCGLICACAVPYRGGARAVQPTQLDETSWFRA
                     APTPVVRQKQMTDCGLAALAMVAGAWGRNWSIDDLAHRVPPGKHGIKLGILRDLARSR
                     GFEAYAIKANREDLKSELSAGRPVLLGLLLPHDRKRNRSHYEVAIAMNRADGTVITID
                     PATGEWMRRSPAVLDLEWKAAGYAALVVTADKAQETASK"
     gene            complement(16492..16668)
                     /locus_tag="VMZ53_26230"
     CDS             complement(16492..16668)
                     /locus_tag="VMZ53_26230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32037.1"
                     /translation="MEVVKTEAAAPKAADSHNYAQREAQDKKAADFQGGDLLIVGISG
                     GAIIVLLFLLLILA"
     gene            complement(16840..18483)
                     /locus_tag="VMZ53_26235"
     CDS             complement(16840..18483)
                     /locus_tag="VMZ53_26235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HUS32038.1"
                     /translation="MPARLRRSRHLSWFTHMGAVYLFHDLYGYLMEMSPDIAAMIEAF
                     DDGVDTQETIAYYQGKFGDGDPKQFVDVLAAHSVLVEPNEDEIEGLWAFVPIKAKWNV
                     WQRREDRLTLWTAWGQKPVQQVFLDPDETKIWDAFAGEKRLIEMRHHYDNDKLIKLVQ
                     RLTHSDVQALKLSMMPWSMYAKRPSLAPPYLTSTMPYERWTPGSPVPPAVSLTDYYRH
                     DVGDADLQFDHLETTLSHLFRVPHPALGNRTYGQTLIDALIARDAIPAGTIRVLEIGA
                     GLGYFARHVIDRLREKGRTVEYSILELAPALITAQKAKVGADAKWIEGDALTVEVPAG
                     SFDLVLCNEMVGDLPAKQLSRLDIGLDLSGTGNADRAKVKAISPIAEACNLDDAPEPF
                     YLQTGAFELIARIAKWLAPGGTAVVTEFGDVAQWPRLSTHLDHPELSTHFGHLQQVAH
                     GESLEAKIEFVIDLLDMDRAQEGLATTRSHFRALRALAEHLGIDLPKIGYTRELLEHT
                     VAGKLDLNLVGDLRFERIEDRLMGLVPHEFKALLAQKPR"
     gene            18537..19685
                     /locus_tag="VMZ53_26240"
     CDS             18537..19685
                     /locus_tag="VMZ53_26240"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS32039.1"
                     /translation="MAYLFESEEHAAIRATCRKFAAAHIAPRGAEWEESEEFPVELYK
                     TAAAAGIQGIGYPESVGGQGGDLSHVLVACEELILAGRSVGTIVGLGTHGIALPPIVR
                     FGTPEQQARFVTPCLRDGKISALGITEPGGGSDVASLTTKAVRDGDHYVITGAKTFIT
                     SGTRADFVTTAVRTGGDAHGGISLVVIERGTPGFEVSKKLKKTGWWASDTAELRFESC
                     RVPVANRIGPENGAFPMIMMNFVGERLALAGQCVAIAELAYRESITYARERNAFGKPL
                     SGFQVTRHKLADMASRIAAARALTGEVCARALKANDPSLAGLAAKAKNVATDMCSFVC
                     DQAVQIHGGYGYMRETLVERLYRDARLYPIGGGTREIMNEVIAKTEGY"
     gene            19689..20726
                     /locus_tag="VMZ53_26245"
     CDS             19689..20726
                     /locus_tag="VMZ53_26245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32040.1"
                     /translation="MRRVPSTLLVAVVARVVFTVARFCISGAYAPELSLAGSGVTLAY
                     DVLVLAAMLEVRRLERAGSREWQAWPAAVPIAVDLLYLLTGTSASRADVIAAVGGIAL
                     VVYVARLPQIALASQRSERTVASKAMQATTRVAVMWTIFGVVLFMLTTIRTYELAAIR
                     MHAMTYAFTYAMCLGLLGYALGRAASQLSQWTAAIAAWLMVWCAGVLAAQLPWIYRMF
                     WQTSSWTDVPAARYATAFVNGLPVAVMLACALFSLSVAALGSRHGDKDLVTEARGRGI
                     AIVLMFLASQRAVTWLAPKLGDELTYVAVMAAAAILNALAAYQLARLWDSTGRSLAEP
                     AVPAARVVTAP"
     gene            complement(20713..21603)
                     /locus_tag="VMZ53_26250"
     CDS             complement(20713..21603)
                     /locus_tag="VMZ53_26250"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HUS32041.1"
                     /translation="MRLLTAALPLLVLCSEASADRHVVQKGETLEHIARAYGCSVDAI
                     ERANSVKTTLLTPGTSIDIPACSLRSRAQVRSTSAAKPRARATDDDDDKAKAALAVID
                     GATWQPRPAAPSGITGGFAALAQGDGYELRRPTRAYGEPHVIEHLRSTIAVVRALYPD
                     VHTLAIGDISAAHGGKLDNHKSHRTGLDVDVGFYFKKVPDGYPDHFVAAGKDLDIEAT
                     WALLTAFARTSQADDGVEIIFLDYAVQKRLYDWARARGTPDDDLADLFQYPRGKDSLS
                     GLVRHWANHSDHLHVRFKAQ"
     gene            21735..22121
                     /locus_tag="VMZ53_26255"
     CDS             21735..22121
                     /locus_tag="VMZ53_26255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32042.1"
                     /translation="MPDLASSSSSVVDLGPPRWKKIVVIGGGITAVAVVAAVALKLIF
                     TVPGGAACDHLDELPGGDQVVQRLENYVESHVVEAGLVREQRHEVSGCRAALSALDQT
                     MTHGRFTRMTECIEKAKTAADASRCI"
     gene            complement(22149..22697)
                     /locus_tag="VMZ53_26260"
     CDS             complement(22149..22697)
                     /locus_tag="VMZ53_26260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32043.1"
                     /translation="MANVIEEAKSGRASCRTCKKTIAKGELRFGEEAPNAFGDTPSIR
                     WHHLLCAAEKMPAELKAVLDTYSDPVPNRAELDKAMQDAMSKGRAKPGGFPYADKAPT
                     GRAKCMQCEEPIAKDTLRVAVEREIDTGAMVTRGAGYLHPKCVAENLENVGGSKDDLV
                     EGLRTNSRLVPADLDQVIAEIG"
BASE COUNT         3950 a         7401 c         7742 g         3749 t
ORIGIN      
        1 cgtggacgtg gacgtggacg tggacgtgga cgtggacgtg gacgtggacg tggacgtgga
       61 cgtggacgtg gacgtggacg tgcagcccgc gaactaggtc ttgagctcga gcggctgacc
      121 gttttcgcga aagtccatcg agaccgagtt caggcaatac cgtaggcgtg tcgggggcgg
      181 gccgtcgtcg aagacgtggc cgaggtgtgc atcgcacctc gcgcagcgga tttcgacgcg
      241 ccgcatcccg tggctctcgt ctgcgagctc ggtcacgtgt gcgggatcgt atggcttgaa
      301 gaagctcggc catcccgtcc cggactcgaa cttcgagtcg gaggagaaca gcggtaaacc
      361 gcacaagcgg cacgtgtagg tgcccggttt cttgttgtct agaagcaggc cgcagaacgg
      421 gcgctcggtg ccctgatgaa ggatgacgtg gcgctcctcc ggggaaaact ccgaggcaag
      481 cgcctggatc cgatcgtcgg caagccgcgt gacgtcgtag ccggatttcg aggtgggcat
      541 ggcgcatttc tacgcccaaa cccgagtcga agttacgcgc gaaatgccgc caatcggttt
      601 acgatggtcg acgaatgaag ggtcgccttt ggggggcagc ggctggcgtt gttgcgctag
      661 gactcaccgg ctgtttcgac gagcagatca cgcactgtcc gacggtcgat tgcccgatcg
      721 agatggtgtg cgacggcgtc ggcggctgcg cggttcccga acagctctcg cagtgcagcg
      781 ggcagacgga cggcgcggcg tgcagctttt cgacgctgtc gaacgtccat atcgacggtg
      841 cctgtgagac gggcgtgtgc cgcagtcgcg agattccggc gtgcttgttc gatcagttcc
      901 tcgacagccg cgtcgacgtc ggcatgtggg agctgtggct gccgtcgaac gaaccgatcg
      961 tcgtgtcgga agagccgggg cgcctcgcga tcgcgatcgc accggacgtc ggtcgtctct
     1021 ataacggcat ccagagtcgt ggtcgctacg acatgctcgg cggcaacgcc agcgtcgagg
     1081 tcgagcccgc cggccaagag atcggcgtcg agacgacgtt cgccgtcgat atcgattcga
     1141 cgctcggctt cgagatgtct gcgtacgcag atcgattgca cctcgtcgtg cactcgagcg
     1201 gcggcgtgac gaactcgatc cccgtcgatt acgaccccgt cgcgcagcgg ttctggcgat
     1261 ttcgccatga caccggggcc ggaacgatgg agatggagac gagccccgac gggaacaact
     1321 ggacgtcgca gcgtagtgca gcgctcgccc gtccgccgac aggcgtggtc gtcacgctcc
     1381 tcgcaggtac atatatcgac cgcggcgccg catccccagg cgttgcgtac ttccatcggg
     1441 tgaagctaac gagcgctacg tgtccttgag ctcgcgctat gtgagcgcgc tcttgacgtc
     1501 tttcgtcgcg gcgttcatcg cctttggcgc tgcgtgcggt agcccgaacg caacgacgga
     1561 cgcgcggccg ccacaacgcg acgctccccc tggctccggc cagctcggcg acgattgtgc
     1621 gcagcattcc gattgcgccg gcttctgtgt cgaggccgtt ggcggcatcg gcggcgtgtg
     1681 cacgcgcgag tgcaatgatg actgtcccgc ggagtggacg tgtcgcgaga tcgagatctc
     1741 cggcacggcg gtccgcgctt gcgtccccga cgctccgcag ctgtgtctcg catgtgtcga
     1801 cgacgccgag tgcggcaccg gcgctgcgtg tctcacgatc gacaatcagg gctcgtgcgc
     1861 gacgaagtgc acggtcgatt gtcccgccgg gtacacgtgc gcggtcgatc cgaagggcac
     1921 gcacccgggc aactactgcc agccgatcac cggcagctgt acgtgcagcg ttggaatgga
     1981 cggcatgagc cgcgcgtgca cgaacacgaa cacgatcggg acgtgcttcg gtacgcagct
     2041 ctgcaatccg ctcgttggct ggtcgacgtg caccgcgctc gcggcagcgc tcgaagattg
     2101 cgacggcaac gacaacgact gcgatttctt gatcgacgag gacaccggcg gcgggcaggc
     2161 atgctcgaac accgtcgcgg gcatcggcac gtgcgcgggt actcgccaat gcggcggctc
     2221 ggtcggctac gtctgcgaag gccagattcc aagcgccgag aagtgcaact acgccgacga
     2281 ggactgcgac acgagggtcg acgagacgtt ctcgggcctc ggcacgatct gcagccccgg
     2341 cgtcggtgga tgccagcgcg tcggcagcgt gcgctgcaaa gcggatggca ccgccaccga
     2401 atgctccgtc gtcgcgggca cgcccacgag cgagctctgc aatcgcgtcg acgacaactg
     2461 caacggcacc accgacgaaa cattcccgac gctcggcaac agctgctcgg tcggcgccgg
     2521 tacgtgccag cgcttcggca ccacggtctg cgcgacgaac ggcacctcga cgacatgctc
     2581 ggtcactgcg ggtaccccga cgacggagct ctgcaacgcg ctcgacgaca attgcaacgg
     2641 cacggcggac gagacgttca cgatgctcgg caacggctgc gcggtcggca ccggcgcgtg
     2701 ccagcgcttc ggcacgacgg tctgtgcgag caatcaaacg tcgacgacat gctcggcgac
     2761 cgcaggttct ccgacggcag agctgtgcaa cgcgatcgat gacaactgca acggcatgaa
     2821 cgacgagacg ttcacgacgc tcggcaacgg ctgctccgcc ggtatcggcg cgtgtcagcg
     2881 cttcggcacc accgtctgcg cgagcaatca aacttcgacg acgtgctcgg tcgtggccgg
     2941 ctctccggtc gccgagctct gcaacaagat cgacgataac tgcaacggct cggcggacga
     3001 gacgttcacg acactcggca acagctgctc ggtcggcatc ggcgcctgcc agcgcttcgg
     3061 cacgactgta tgcgcgggta accaaacgtc gacgacgtgc tcggcgaccg caggcactgc
     3121 cggcaccgag ctctgcaacg cggtcgacga caactgcaac ggctctgtcg acgagacgtt
     3181 cacgacgctc ggcaacgcct gctcggccgg cttcggcgtg tgccagcgct tcggcacgac
     3241 ggtgtgcgcg agtggtggca tgacgaccgc ctgcacggca accgcgggca cgaattcctc
     3301 gtcggagacc tgcaacttcc tcgacgatga ttgcaacggc gtcgtcgaca acggcttccg
     3361 caacgcgggc accggcgtct acgatcaaac cgcgaactgc ggctcgtgcg gcaacgactg
     3421 cgcggcgatc tacaccggtg cgaactcggc cgggcagtgc agcacggcga ccggctcgcc
     3481 gacgtgcgtg atggtctgca gcgcgggcac gagcgacctc aacgcgtcga gcttcgacgg
     3541 ctgcgagttc gtgctcgaca acacgaccgt gtacgtctcg accacggact ctgcggccgc
     3601 tgatgacgcc acctgcggcc ttggcccgac gggcagcggc gccggcaatc atccttgtaa
     3661 gacgatcacg ttcggcctga cgcgcgcggg cactctcgct cgcccgaacg tccgcgttgc
     3721 cgacggtacg tacaacgagt cggtcacgct cgtcagcggc aagaacgtct acggcggcta
     3781 ccgctcgggt acgtgggatc gccatctcgc gacgacgtcg acgctgatcc aaggtgtgag
     3841 ctcggtcggc gttcacgacc gaacgatcgt cgcgacgaac gtgagcaacg cgatcttcga
     3901 aggcttcgtc gtccgcggct ccttcaatac gaaggcaacc ggcaactcgt acgcgatcta
     3961 catctccggt ggcgcgtcga cgctcgtgat caaagacaac gtcgtcttcg caggccgcgg
     4021 tggcgccggt tccgaaggcg gagcgggcac ggtcggctcg acgggcccca acggcacggg
     4081 ctcggtcaac ggctcgtacg acggctacgt cgccggcagc ccgccttgtt cggcgagtcg
     4141 ccagttcaac aacggcgctg tcttctcgtg cggcggcgat aacgtgagcg gcggaaacgg
     4201 cggcggcaac aagtgccgtc ccgcgaccga ctacacgcaa ttcagcggca tcaacggatt
     4261 cggcggccag cccgccgcag gtgcaggcgg tggcgctgcc ggcggcacgt cgcaaggcgg
     4321 cttcgacggc acgatgcaga cgcagggaca gaacacgacc tgcttcttgc ccgggccctc
     4381 catggcaggc gcggacggcg catcgggtat gtcgggcagc aacggcagcg gcgtcgctgg
     4441 ttgctcggcg tcgacgggca ccgtgagctc gggtgagtgg gtcaacggag ccgcgccctc
     4501 gggcgtcgtc ggattcaatg gcggcggcgg tggtggcggt gcagcaggtg gcggcggcgc
     4561 gtgtacggca tgcggtggaa gcctcaatcg cgatcgcctc ggcgcacatg gtggcggcgg
     4621 cggcgcaggc ggctgcggcg gcgcaggcgg caacggcggc accggcggtg gtggcgtctt
     4681 cggcatcttc atcgtcggtg gcaccgcgcc gacgctcacg aacaactcga tccaacgtgg
     4741 cgccggcgga cccggcggcg acggtggcat cggtggcgct ggtggcttcg gcggacgcgg
     4801 cggtgcaggc ggcgcacaag tcatgctctg cgcggcaaag gccggcgtcg gtggtaacgg
     4861 cggcaacggc ggttatggct ccggcggcgg cggcggttgc ggtggctcga gctacggcat
     4921 cttcacgtcg ggcatcggta cgccgacgta ttgcaccaac aacacggtgt cgggcggatc
     4981 ggcaggtgcc ggcggtgtcg gcgggttctc cgtcggtaat tccggcgcga atggcaccgc
     5041 gggcgtgctg caaggatgta cgtcgatctg atgcggacca cacgttacat cgtgctcgcg
     5101 ttcgcgctct gcttcgtcgg ttgcggcggc tcgaacgaaa agaccgatgc cagcgtctcc
     5161 gacgcgttcg gtggcgcgaa cttcggtgcg ccgtgccgga cggccgaaga ctgcccgggc
     5221 ggcatgtgcg ccgagacgcc gagcggcggc gtttgctcgt cgacctgcaa tggcgcatgc
     5281 cctgaaggct tcaactgccg cgtccgcgac gtcggcggat cgctcgaatc gatctgcgtg
     5341 cctcagctct tcgactactg cacggcgtgc aactcggacg cgcagtgcaa cggcggcgta
     5401 tgcgtcgatc tcgaagggac gaaagcgtgt ctcgccgagt gcccgcatca aggcagctgt
     5461 ccgacgggct atacgtgcgg atcggatccg acgggccagc acatcggcag cttctgcgtt
     5521 ccgctcacgg cgacgtgctc gtgcaccgtg gcagagcagg gccaagtgcg aacgtgcacg
     5581 aagtccaatg tgctcggcac gtgtcgcggc atcgagacgt gcgacgccga caacggcggc
     5641 tgggtcggct gctcggccct cgacgcgacg acggagagct gcgacggctc ggacaacgac
     5701 tgcgacggcc tcatcgatga aggcgtcggc ggcagcccgt gcatgaatac ggtcgccggt
     5761 gtcggtagct gcgctggcgt gacgcgctgc caaggcgcag cgggcgtgac gtgcgaaggc
     5821 aagacgcccg tcgcagagac gtgcaactac gccgacgaca actgcgacgg catgccggac
     5881 gaaacgtacg ctgggctggg caacgtctgc agcgtcggcg tcggcacgtg ccagcgctac
     5941 ggcgtcgttc gctgcaacac ggcgggaacc gcgacggagt gctccgtgac cgcgggctcc
     6001 gccgtgaccg agctatgcaa cgcgctcgac gataactgca acggctcgat cgacgagacg
     6061 ttcacgacgc tcggcaacag ctgctcggtt ggcgtcggcg catgcacgcg ccagggcaac
     6121 ctcgtctgca atggcgccgg aaccgggacg acgtgctccg tcgctgcggg ctcgccggtc
     6181 accgagctgt gtaacggccg cgacgacaac tgcaacggct cgacggacga gacgttcacg
     6241 acgctcggcc aagcgtgcag cgtcggcgtc ggtgtgtgca cgcgccaagg cagcttcgtg
     6301 tgcaacggcg cgcaaaacgg cgtcgcgtgc tcggtcgccg caggcatgcc cggcaccgaa
     6361 gtctgcaact cactcgacga caactgcaac ggatcgatcg acgaggcgtt cccgacgctc
     6421 ggccaggcgt gcagcgtcgg cgttggcgta tgtacgcgtc agggcagctt ggtctgcaac
     6481 ggcgcgcaga acggcacggc gtgctcggtg acggcaggcg cacccggcac cgaggtctgc
     6541 aactccctcg acgacaactg caacggctcc gtcgacgagg cattcccgac gctcggtcag
     6601 gcgtgcagcg tcggcgtcgg tgcgtgcatt cgccagggca acctcgtctg caacggcgcg
     6661 caaaacggaa cgacctgctc ggtcgccgcc ggctcgccga ccgccgagct gtgcaactcg
     6721 atcgatgaca actgcaacgg aatgacggac gagacattca cgacgctcgg ccaagcgtgc
     6781 accgtcggcg ttggcgtgtg cgcgcgccag ggcaacatgg tctgcaacgg cgcgcagaac
     6841 ggcgccgtgt gctccgtcgc tgcgggcacg gccggcacgg agacgtgcga cacgcgcgat
     6901 gacgactgcg atggcaacac cgacgagggc tggaagaacc agagcaccgg cgtctacgat
     6961 caagcaaatg catgcggcag ctgtgcgaac gactgcaccg tctcgtacgc gctgcccaat
     7021 gcagccggcg tgtgcaacct gaccggcagc ccgcattgcg cgatgcagtg cgcggcgaat
     7081 gcattcgatc tcgacagcgc gctcgcgaac ggctgcgagc tgctgctcga cacgagcgct
     7141 gtctacgtct cgactgacag cgccggctcc gccgacgacg cgacgtgtgg tcaaggcccg
     7201 caaggcacgg gcagcggctt ccatccgtgc aagtcgatca cgcaaggtct cacgcgcgca
     7261 gcggcgctcg gtcgtcctcg cgttctcgtc gcgaacggca tctatcccga gtcgttcacg
     7321 ctcgtgaacg gacgcagcat gctcggtggc tacaaccccg actcgtggct gcgcaacgtc
     7381 gccacgacga gcacgtacat cacgggctcg aacacgatcg cgacgagcca cgactacgcg
     7441 gtcagcgcga tcaacatcac cagcgcgacg gtgttcgaag gcttcgtcgt cgtcggcccc
     7501 gtcaacagca aggtcggcgg caactcgtac gcgatctaca tctccggctc caactcgaat
     7561 ctctcggtga tcaacaacgt gatcgcgtcg ggtcgcggcg ggcctggtag taacggctcg
     7621 tcgggcctca gtgggtcgac gggcgtcgat ggaaccggcc gcagctcgaa cccgacggcc
     7681 tacgatacga aggacgcggc cgcttcgccg tgcaccttgc cgactcgcca gtacgccaac
     7741 ggcggcacac tgtcgtgtgg aacgaacgtg gtcagtggcg gaagtggcgg cggaaaccgc
     7801 tgcgcgccga agaacgacta cacgatcttc agcgcgttca cggggtcgac aggtaatgca
     7861 ggcgtgggcc tgctcggcgg cgccgcaggc ctaggcggcg aggccggtca cgacatggag
     7921 ctcgccgtcg atgcaggcgg cgacatttgc tacatcagca cgacggcggg ctggcacgac
     7981 tacggcttcg acggaaaaaa cggttcgagc ggcctcaacg gcaccggcgt ggctgcatgc
     8041 acggcggctg gtggctcggt ctcgggcggc cattgggtcg gtggctccgg cctcggcggt
     8101 gtcggcggcg gcaacggtgg cggtggcggt ggcggcggcg gcggtggcgg cgcgacgtgc
     8161 gtaggatgcc cgagcggtca cgacgagctc ggcggtcacg gcggaggtgg tggcgcaggc
     8221 ggatgtggcg gcacgggtgg cggctcgagc accgctggtg gcggcgtctt tgcgatcttt
     8281 gtcgtcggcg gtgcatcggc tccggtcatc accggcaaca acatccagcg cggtcaaggc
     8341 ggcgcgggtg gacgcggcgg cgcagctggc gtcggtggcg ccggcggctt cggtggcgac
     8401 ggcggtctcg tcgcatcgcc gatgttctgc ccggcgctcg caggtcgtgg cggcaacggt
     8461 ggcgccggtg gtcacggttc gggcggcgca ggcgcgtgcg gcggcgtgag catcggcatc
     8521 tacacgagcg gcctcggcgc gtcgatgaac tactgcctcg cggccggcaa caacacgatc
     8581 accggcggtg cgtcgggtcc tggcggtccg ggcggctact ccgtcgtgaa cgtcggcccg
     8641 acgggggcca acggctcgtt gtccgcctgc tcgttcaact agaacgacta cattgtagta
     8701 cccacagccg atcctcgggc gtcagaagcc tgatgttaga attcggcaga ggtggctgat
     8761 cttcctgaag cacccgacga ggacgcgtcg acgaacgtca acacgcctcc gccgggtcgg
     8821 cccgcgctca agcgccggat cacgcggccg agctacatgg tcgagctcac cgagggcagc
     8881 atggtcggcg agtacgaagt gcaatcgcag atcggcgaag gcgcgatggg caccgtgttc
     8941 atgggcgtgc atccgctgat cggcaagcgc gtcgcgatca aggtgctcaa gcccgagctg
     9001 tgcgcgaacc aagcttcgat cgatcgcttc gtgcaagaag cgcaggcggt caacaagatc
     9061 ggccacccca acatcgtcga cgtgttctcg ctcggcgagc tcgaggacgg acgcgcgtac
     9121 ttcgtcatgg agtttctccg cggcgaagac ctcaaggtga agctctcgca cgggccgatg
     9181 ccggtcgccg agacgtgcga catcctcgac ggcattgcac gcgctctcga ggccgcgcac
     9241 gccaaagaga tcattcatcg cgatctcaag cccgacaacg tgttcctgca tcaggtcgac
     9301 aacggcccga tgatggtgaa gctcctcgac ttcggcatcg cgaagctcgt gcgcacgaac
     9361 cccgggcagg agaagacgca gacgggcaac atgctcggca cgccgcgcta catctcgccg
     9421 gagcaagcgc gcggcattca ggtcgatcat cgctccgata tctattcgct cggcgtgatc
     9481 gcgtacgaga tgctcgccgg tcggcccccg ttcgacggcg agaccgcgat ggacctcgtc
     9541 gtgaagcacc tcaacgagga cgcgccaccg ctgtcccaat tcgtgaaggt gccgaggaac
     9601 ctcgagcagt gcgtgatgcg catgctcgcc aaggacgcga aggcgcggcc gtcgctcgag
     9661 gagattcgcg gaatcctcgt cgatccgacg cggcggctca cgccggtacc gcgctcgacc
     9721 gcacacgcga tgccgaagtc gccggcgaag ccccgctggc cgctcctcct tggcgccgtc
     9781 gtgctcgtcg ccgctgcggg tgtcgcgacg tggaagctcg tgctcgacaa gggtgacgac
     9841 aaacctgccg cggtggcgag cgaagcgccg gttcccgtcg agaagcaagt ccccgaagtg
     9901 aagcagcccg aacagcccgt gcagccagcc gcgagcgcac ccgcacccgt cgaacgcggc
     9961 acgctcgagg tcgaggtcaa aggtgcgaag gactcgacga tctacgtcga cggcgaagaa
    10021 tgggggcgcg gcgcgtctgt gaaagttccg ctcgaaccag gcacgcacga gatcaaggtc
    10081 aagccaccgg gtcgcgctgc gatcgtgcag acgacgacga tcgaatcagg acagtcgaac
    10141 aagctcacca tcgtcgtgcc gaaggagaag acgatcgtga tccggcgcgg cagcgcgaag
    10201 acgcagccga aagagacgcc gcctccgccg ccgagcgacg atgcactcct cgctccgaaa
    10261 aagaggaagg ggaagtgaag tcactcgtca tcgtctgcgc gctcgcgggt gccgtgtatg
    10321 ccgcaccgaa agagaagccg aagaaagtcg acgtcgcgca gacccaagcc gatgcgctct
    10381 acgagaaggg acagcagagc tatcaggcgg gcgagtacca ggaggcgatc gagcacttca
    10441 agcgcgcgta cgagctcgtc aaggacccgg tttatctgtt caacatcgcg cagagctatc
    10501 gcaaggtcgc cgactgcctg aacgcgcacg cgttctacac gcagtacctg caagaagcgc
    10561 cggacgcgga gaacaaggac aaggtcactc agtggctcag cgagctgcag ccgtgcgtcg
    10621 accagcgcca caacgagcag gaggcggcac gtcgcgcaga ggaagcagac cgcgtccgca
    10681 aagcagacga tgcacggcga gcagccgaag cgcgcaagcc ggttcccgtc gaggtcgatc
    10741 ggggcggcgc gctgcgtatc gccggtatcg cgacgggaac ggtcgggctc gcggtgctct
    10801 ccgctggcat cgtctacagc atcaacggcc ggagcatcaa gaacgacctc gcggatcggt
    10861 gcagcatggg ctgccagtgg gactcgatgg agatcctcga cctcgacgcg cagggcaagc
    10921 gggcgaacac ccgcgcgaag atcggcacga tcgccggcgg catcgcgacg gtcgtcggtg
    10981 tcagcctgta tatcgtcgga cgcacgcgcg tcgagaccgt gatgatcacg ccgaacgccg
    11041 gcggtgccac ggtgagcgct cagctgacat tttagctatc cttcgatcgt gccgggtgac
    11101 cgagagccga gcgaggacga gctcgcacgc accgccacgg ttggcgcgac ggccaccgtc
    11161 acgggcgaac cgggccgcga cgtgcctccc gtgcgcgact ctgacgtcgg cgatcagctg
    11221 gggcgctaca agctgcagcg cgagatcggg cgcggcggca tgggcgtcgt gcacgccgca
    11281 ttcgatccgg acctcgagcg gcgcgtcgcg ctgaaggtct tgcccgttac gagcgagagc
    11341 gtcgaggcac gccagcgatt gctgcgcgaa gcgcgcgcga tggcgaggct ggcgcatccg
    11401 aacgtcgtca cggtgcacga ggtcggcacc gcgaagggtc gcgacttcat cgcgatggag
    11461 ctcgtcgacg gcaagacgct cgaagactgg ctcagcgcaa agccgcgcac ggagcgcgag
    11521 atcatcgagg catatgtcgc cgccggacgc ggtcttgctg ctgcccatgc agcggggctc
    11581 gtgcatcgcg acttcaagcc gcgcaacgtg ctgcgccatc gcgacggccg catcgccgtc
    11641 accgactttg gtctcgtcgt cggtgtcgag gcacaagccg atgccttcac gacgacgctg
    11701 ccgtcgggcg gcctcgctgc gctcgacgag acgacgacgc cgagctcgtt gtcgggactg
    11761 acgaagaccg gctcggtgct cggcaccccg gcgtatatgg cgcccgagca gtggaccggc
    11821 ggaacgatcg ggccgcccgc cgatcagttc gcattctgcg ttgcgctctg ggaggcgctc
    11881 gcgaaggagc gtccgtatcg aggcgatacg ctcgaagcgc tcaaggaaaa ggtgatcgcg
    11941 ggcaggcgcg cggacgattc gaagattccg cgcaagctcc gaggtgtcct gcggcgcggt
    12001 ctcgatccgg atccggcgaa gcgctacccc gacatgaatg cgctgctcgc ggcgatcgtg
    12061 cggaccgaac gccggccgac gctgctattc gcgctcggtg cggcagcgat cgtcgcggcg
    12121 gtggtcgtct acttcgtcgc agctcgttcg ccgaccgtac agaagacgta cgtcgtcgga
    12181 gaacgctgtt ccgcacccgt gctcgcgccc gacaaggtgt ggaccgcggg cgatctcgaa
    12241 cgcttgcgag ccgcgcaaca gggcctcgca gcggacctca tcgatgcaga catgaaggca
    12301 tggcacgagc tgcgcgctcg cgcgtgcaag gcgcaagcgg acgtacgcga gccgcagctg
    12361 gcgtgcctcg acggcgtgct gtcgcgcatc gataccgtga agcaagcgct cggcattccg
    12421 aacgtgtctc cgcagctcga gatcaacgag ctgctcgtcg atcctgcgac gtgcaatacc
    12481 gcgcagccgc ctcggttgac gcgcgtcatg tcgggcgaga tgcgtgggct gatcgggcgc
    12541 tatctcgtgt cgcagactcc gggcagagag ctgaaggcta gcgatgccgc ggcactcatc
    12601 gctgcggcgg gcggcgagcc gtgtgcactg tcattcgcgc atcagatggc cgtcgggatc
    12661 acccctggct ccgagcgggc agaccacctc gcgagagcgg agcgtgcttc gcaggagtgc
    12721 gccgacgacc ggttgcttgc ggacacggcc atgctgtcgt cgatgatcgc aggtttgctc
    12781 gcggagaacg agcttcgcac tggtctgcag cgctcggagg cgctcgcggc tcgcgtgtcg
    12841 gagccatttc tcgaggcgca actcgagcag cagagggtgt cactcgcgat gatcagcgac
    12901 gacatcgacg gcgcggtcgc tcacagcgag aaggcgctcg agctgtttcg agatgccggc
    12961 gctgttcgct ctgcgttgac cgcgcagctc gacctcacgt cctggaagga tcggcggggc
    13021 ggcgcgaacg acttcgcggg tcggggtgaa gagctcgcga cgctgcgcgc ggacatcgtc
    13081 aagaagctcg gcgaatccga ccgcgccgtc gcaaacatcg acgctaggcg cgcggactgg
    13141 atgtggcaga acggcgacct cgccggcgcc gaacagctgc ggcgcaagct ggtccagcgc
    13201 gatcctgcat tcgagccgca cgtgatcgcc ggtcaggtcg tcgacgacaa agggcccgtc
    13261 gaaggagcgg aggtcacggt tgccccgtcg atgtggggtg actcgctgcg cgccgccgag
    13321 acgctcggct ccctgcagcg ctccaccgtg accggagccg atggccggtt ctcgatctcc
    13381 gacgtcgaga cgaagtcgta cctgatcgcg cagcatggtg atcagcgctc ggccgctgtg
    13441 ctcgcgacgc cgaacatgac cgtgatgctg acgccaacga gccgcgtctc cggcaccgtc
    13501 gatctgcgcg gccacgcgcc atcgcaggtg acgctcatca tcagtgcgaa gagccagccg
    13561 aaggaaacgc cctactacat gagagcgccg atcggtgaag acgggacgtt ctccgtcgac
    13621 ggcgtgccgc gcggcgcggt cttgttccag acgagcgtcg ccctgccgac ggcgcggatg
    13681 gttgcgggca aggaagtgaa cgtgagcaaa cccgtcgtcg agggcgtccg gctcgaggtc
    13741 aaggcgagcg cgcgccctct caacgtgctc gtccgtagca tctacggggc accgatcgtg
    13801 aaggcgaacg tcttcttgat cgcgggcaag caaccgccgg atggcttctt cgggaagatg
    13861 ttctcgggca aggagaacta cacgccaaag ctcgcactcc caccacagcc cgaacagcaa
    13921 acgccgatcg ttcgcgcgcg catgaagagc ggcgatctgt tggcgcgctt tgcacaacgt
    13981 cccgaaggcg aagtcagcgt ctgtgcgatg ccgatgccgg atcggctcga cgacccacag
    14041 ttccaagccg cgggcatgga tctaaagcgg ctggagaagt ggccggtgaa gtgcgtgccg
    14101 atgaagtctg acgacgagct cgtggagatc gcgatcccac cgctgccgcg gttcgattag
    14161 cgccgattag cgcaggtccg cgccgtcgac gatgcgatcg tgctcgggag cttcgccgag
    14221 ccggatcagc gcggacgcga gcacgtcggg cgtcgtcgag cgatagcgcg ctcgcaagct
    14281 cttggcgccg aagacaccga cgaccgcgag tagaccgtcg ccgatcacgc cggcggtctt
    14341 ctcaccgagg cggctgtcgt cgcgctcgcc ggtgatgatc gacggacgcg cgatcaccca
    14401 cgccagccca gagcctttca ccgcgtcttc tgctttgccg cgccacgcga gatatgccga
    14461 gcgcgccttt ggatcggcgc cgaccgagga caagtagacg aggcgaggcg agacctccga
    14521 gccgcgcgcc gcgtcgaccg cgagcttcgt cagaccgaag tcgaccttct cgtagatgtt
    14581 gccttcgatc gagtcagcct tcgctttgct gcgtgtcgtg ccgatgcaga tgtagatctg
    14641 cgcaggcttc agctcgcgcc agcgcgctgc aagcgccatc gcgtcccacg gcgtggtgtc
    14701 gacctgcgca ccgagctcgc caaagcgcgt ctgccactcg gcgagctgct tcgaatcagg
    14761 acggacatgt gcgtgcgctt tgacgccgcg cacacatagc tgccgcacga cctcgcggcc
    14821 gacgaagccc gttgcgccga ggacgaaggc cgtcatggct acgacagctt cgcgaggccc
    14881 gcttgcatgc gcgccttcag cggttcgacg actgccgggt cctttacgtt gatgaccggc
    14941 tcggtgtcgt actgctcgta gaagaccgag tctttcgtct cgggcggaac gccgagcgct
    15001 tcgcggatgc gcttgaagtc gggcacgaag ccgcgatcca tgagcgggat gatcgtgccg
    15061 gtgatcgtgc cggtgagacc gcagatgaag atgacgacgt tcttcggcgt gaagccgccg
    15121 gccggcagac cgacgcgcgt ctccagatcg tcgatgcgac cggattcgaa gaacgcttcg
    15181 acgcgcccgg tatcgccctt ccagctcggg gcctctttcg ggcggctgac ggtgccccag
    15241 tacttgagcc cgttgcgctc gaccatctgc atcagctcgt cgcgatagcc gaggtcgtcc
    15301 ggatacgacg cgccgtgcag cagcacgtac ttcgacagat ccgcgtccgg attcttgcgg
    15361 agctcgctgc gcaccatcga gagaaacggc gccgagccag tacccgcggc gacgcacacg
    15421 cgcaggcggg tgtcggcctc gccgatggtg tccttcaccg tgaacacgcc cgcggcgaca
    15481 ggacgcatgt acatgcgatc gccggtcttg agcttccaga gcaagtgcgt cagcgggttc
    15541 tcggactccg gcttcgagac gaagcggatg tagaactccg tcgggcccgc gtcttccggc
    15601 gccgatgcga tcgacatcga gcggcgcacc gacccgagct cgggtttctc cgcattgttc
    15661 atgccgagga cgcagtactg accgggaaca aaccagggcc tggcgggctc ctggtccggt
    15721 ttcacacgaa agatggtgag ggcatccgtt acatcgatgc gctctgcgag tgttgcgttg
    15781 tactcgagtg ccttggccat cggcggcgac gctagcacga gggtcgcccg aaaatggaaa
    15841 acgccggatt cattccggcg tcggggacga gctggccgcc cgtcgctact tggacgcagt
    15901 ttcctgcgct ttatccgcgg taacgacgag ggcggcgtag cccgctgctt tccattcgag
    15961 atcgagcacg gcgggtgacc gccgcatcca ctcgccggtc gccggatcga tcgtgatgac
    16021 ggtgccgtca gctcgattca tggcgatcgc gacctcgtag tggctgcgat tgcgcttgcg
    16081 atcatgcgga agcaggaggc cgaggagcac cggccgtccg gccgagagct cactcttcaa
    16141 gtcctcgcga ttcgccttga ttgcgtacgc ttcgaagcct cgagaccgcg cgaggtctcg
    16201 caagattccg agcttgatgc cgtgcttacc cggcggcacg cgatgggcga ggtcgtcgat
    16261 cgaccaattg cgcccccatg caccggcgac catcgcgaga gccgcgaggc cgcaatcggt
    16321 catttgcttc tgccgaacca ccggagtggg tgctgcacgg aaccagctgg tttcgtcgag
    16381 ctgcgtgggt tgaacagcac gagcgccgcc ccggtaagga acggcgcacg cacagatcaa
    16441 cccgcacagc gcgagaactc gcgccgagcg gatgatcgtt ctcatgttcg attacgccag
    16501 gatgaggagc aggaacagca ggacgatgat cgcgccgccg gagataccga cgatgagcag
    16561 gtcaccacct tggaagtccg cagccttctt gtcctgagcc tcgcgctgcg catagttgtg
    16621 cgagtcggcg gccttcgggg cagccgcttc cgtcttcaca acttccacag cggccggggc
    16681 ctcgggcgcc gcgtaagcaa cagcgggggc gccgagagcg agagtcgaag cgagagcgag
    16741 attacgaagc aacattgtct tcctccaaac aggttgatcg gaggaagtgc agtcgtcatg
    16801 cccgcgtcgt tctggctgcg tgggcgctcg ctgtgctccc tagcgcggtt tctgagcgag
    16861 aagtgccttg aactcgtgag gcacaaggcc catcaggcgg tcctcgatgc gttcgaaacg
    16921 aagatcaccg accagattga ggtcgagctt gcccgccacc gtgtgctcca aaagctcgcg
    16981 cgtgtaacca atcttcggga gatcgatccc gagatgctcg gcaagggcgc gcagtgcgcg
    17041 gaagtgactg cgcgtcgtcg cgagaccttc ctgagctcga tccatgtcga gcaggtcgat
    17101 gacgaactcg atcttcgctt cgaggctctc gccgtgcgcg acttgctgca gatgcccaaa
    17161 atgcgtcgac agctcgggat ggtcgagatg cgtcgacagg cgcggccatt gcgcgacgtc
    17221 accgaattcg gtcacgacgg ccgtgccgcc cggcgcgagc cacttcgcga tccgcgcgat
    17281 gagctcgaat gcgccggttt gcagataaaa cggctcgggc gcgtcatcga gattgcacgc
    17341 ctcggcaatc gggctgatcg ccttcacctt ggcgcggtcc gcgttgccag tgcccgagag
    17401 atcgaggccg atatcgaggc gcgagagttg cttcgctggg agatcgccga ccatctcgtt
    17461 gcacagcacg agatcgaacg atcccgcggg aacctcgaca gtcagcgcgt caccctcgat
    17521 ccacttggcg tctgcgccga ccttcgcctt ttgcgcggtg atgagcgccg gcgcgagctc
    17581 gaggatcgaa tactcgacgg tgcgaccttt ctcgcgcagg cgatcgatga cgtggcgcgc
    17641 gaagtagccg agccccgcgc cgatctcgag cacgcgaatc gtgcccgcgg ggatcgcatc
    17701 gcgtgcgatc agcgcatcga tgagcgtctg gccgtacgtg cgattgccga gcgccggatg
    17761 aggaacgcgg aacaagtgcg acagtgtcgt ctcgagatga tcgaactgca ggtccgcatc
    17821 gcccacgtcg tggcggtagt agtccgtgag cgacactgcc ggcggcacgg gggagccggg
    17881 cgtccaccgc tcgtacggca ttgtcgatgt gaggtacggg ggcgctagcg acggccgctt
    17941 cgcgtacatc gaccacggca tcatcgacag cttcagcgcc tgcacgtcgg agtgcgtgag
    18001 ccgctgcacg agcttgatga gcttgtcgtt gtcgtagtgg tgacgcatct cgatcaggcg
    18061 cttctcgccc gcgaacgcgt cccagatctt cgtctcgtcg ggatcgagga acacctgctg
    18121 cacgggcttt tggccccacg cggtccacag cgtcagacga tcttcgcgac gctgccaaac
    18181 gttccacttc gccttgatcg gcacgaacgc ccacaggcct tcgatctcgt cctcgttcgg
    18241 ctcgacgagc accgagtgcg ctgcgagcac gtcgacgaat tgtttcggat cgccgtcgcc
    18301 gaatttgcct tggtagtacg cgatcgtctc ctgcgtatcg acgccgtcgt cgaacgcctc
    18361 gatcatcgct gcgatgtccg ggctcatctc catgaggtag ccgtaaagat cgtgaaacaa
    18421 atacacggca cccatgtgcg tgaaccacga caggtggcga gaccgccgaa gacgggccgg
    18481 cacgctcgga gattgcatgc cctttggtac cactgctggg cgtatgctcg cgcgccatgg
    18541 cgtacctgtt cgagagtgag gagcacgcgg ccattcgcgc gacctgtcgc aagttcgcgg
    18601 cagctcacat cgcgcctcgc ggcgctgagt gggaggagtc cgaagagttc ccggtcgagc
    18661 tgtacaagac agccgcagcc gctggcatcc aaggtatcgg ctatcccgaa tccgtcggcg
    18721 gccaaggcgg cgacctctcg cacgtccttg tcgcgtgcga agagctgatc ctcgcgggtc
    18781 gctcggttgg cacgatcgtg gggctcggca cgcacggcat cgcgcttcct ccgatcgttc
    18841 gcttcggcac gccggagcag caagctcgct tcgtcacgcc ctgccttcgc gacggcaaga
    18901 tcagcgcgct cggcatcacc gagccaggtg gcggcagcga cgtcgcatcg ctaacgacga
    18961 aggccgtgcg cgacggcgac cactacgtca tcacgggcgc taagacattc atcacgtcgg
    19021 gcacgcgcgc tgacttcgtc acgaccgcag tgcgaaccgg cggcgacgcg cacggaggca
    19081 tctcgctcgt cgtgatcgag cgcggcacgc cgggcttcga agtcagcaag aagctgaaga
    19141 agaccggctg gtgggcgagc gacaccgccg agctgcgctt cgagagctgc cgcgtcccgg
    19201 tcgcaaatcg catcggtccc gagaacggcg cgttcccgat gatcatgatg aacttcgtcg
    19261 gtgaacgcct cgcgctcgcc gggcaatgcg tcgcgatcgc ggagctcgcc taccgcgagt
    19321 cgatcacata cgcgcgcgag cgcaacgcat ttggcaagcc gctctccggc ttccaggtca
    19381 cccgccacaa gctggccgac atggcatcgc gcatcgctgc cgctcgcgca ctcacgggcg
    19441 aagtctgtgc tcgcgcgctg aaggcgaacg acccgtccct cgccggcctc gcggcgaaag
    19501 cgaagaacgt cgcgaccgac atgtgcagct tcgtctgcga ccaagccgtg cagattcacg
    19561 gcggctacgg ctatatgcgc gagacgctcg tcgagcgcct gtatcgcgac gcacgcctct
    19621 atccaatcgg cggtggcacg cgcgagatca tgaacgaggt gatcgcgaaa acggaaggct
    19681 actgagcgat gcgacgagtg ccatcgacgc tgctcgttgc cgtcgtcgcg cgcgtcgtct
    19741 tcacggttgc gcgcttttgc atcagcggcg cctatgcgcc cgagctttca ctcgccggta
    19801 gcggtgtcac gctcgcctac gacgtgctcg tgctcgccgc gatgctcgag gtacgccgcc
    19861 tcgagcgtgc gggcagccgt gagtggcagg cgtggccggc agcggtacca atcgccgtcg
    19921 acctgcttta tttgctgaca gggacgtcgg cctcgcgtgc ggacgtgatc gccgcagtcg
    19981 gcggcattgc gctcgtcgtt tatgtcgcac ggctacctca gatagcgctt gcatctcagc
    20041 gatcggagcg cacggtcgcg agcaaggcga tgcaagcaac cacgcgcgtc gcggtcatgt
    20101 ggacgatctt tggcgtcgtg ctgttcatgc ttacgacgat acgcacgtac gagctcgccg
    20161 ccattcgcat gcacgcgatg acgtacgcgt tcacgtatgc gatgtgccta gggctcctcg
    20221 gctacgcact cgggcgtgcg gcgtcgcagc tctcgcagtg gaccgctgcg attgccgcgt
    20281 ggctcatggt gtggtgcgcc ggtgtgctcg ccgcgcagct gccttggatc taccggatgt
    20341 tctggcagac ctcgtcgtgg acggacgttc ctgcggcccg gtatgcaacc gcgttcgtga
    20401 acggattgcc cgtcgcggtc atgctcgcgt gcgcgctctt cagcctttcg gtcgctgccc
    20461 tcgggtcgcg acacggcgac aaagatctcg tcactgaagc gcgaggtcgc ggcattgcga
    20521 tcgtgctcat gttcctcgcc agccagcggg cagtgacatg gctcgcgccg aagctcggtg
    20581 acgagctgac gtacgtagcg gtaatggcgg cggccgcaat cttgaacgcg cttgctgcgt
    20641 atcagctcgc gcggctctgg gactcgaccg gccgctcgct cgccgagccc gcggtccctg
    20701 cagctcgcgt cgttactgcg ccttgaagcg cacgtgcaga tggtcggagt ggttcgccca
    20761 gtgccgaaca agcccgctga gtgaatcctt gccgcgcgga tactgaaaca ggtccgccaa
    20821 gtcgtcgtcg ggcgtgccgc gcgcgcgggc ccagtcgtac aggcgcttct gcaccgcgta
    20881 atcgaggaag ataatctcca cgccgtcgtc agcctgtgac gttcgtgcga acgcggtcag
    20941 caatgcccac gttgcctcga tgtcgagatc cttgcccgcc gcgacgaagt gatcgggata
    21001 gccgtccggc actttcttga agtagaagcc gacatcgacg tcgaggccgg tgcgatgcga
    21061 cttgtgattg tcgagcttgc ctccgtgcgc cgcggagata tcgccgatcg cgagcgtatg
    21121 aacgtcggga tagagcgcgc gcacaaccgc gattgtcgag cgcagatgct cgatcacgtg
    21181 cggctcgccg tacgcgcgcg tcggtcgccg gagctcgtag ccatcgcctt gcgcgagcgc
    21241 agcaaagccg cccgtgatcc cgctcggcgc cgccgggcgt ggttgccacg tcgctccgtc
    21301 gatgacggcg agggcagcct tcgccttgtc gtcgtcgtca tcggtcgcgc gggctcgcgg
    21361 ctttgccgcc gacgtcgacc gcacctgcgc gcggctgcgc agcgagcatg cgggaatgtc
    21421 gatgctcgtg ccgggcgtca gcagcgtcgt cttcacgctg ttcgcgcgct cgatcgcatc
    21481 gacgctgcag ccatacgctc gcgcgatgtg ctcgagcgtc tcgcccttct gcacgacatg
    21541 ccgatcggcc gacgcctcgc tgcacaacac gagcaacggg agcgcggcgg tgagtaaacg
    21601 cacgaaaaca atgttcgcgc ggccacgttc ttgtcgccaa tttccgggct tatggaagtg
    21661 agcggcattt tcttggcgat cccgctgaag tttcgctcga agccggcgtc cgaagcggcc
    21721 taagaaacat gccaatgcct gacctcgcct cgtcttcgtc ctcggttgtc gacctcggcc
    21781 cgccgcgctg gaaaaagatc gtcgtgatcg gtggcggtat cacggccgtc gctgtcgtcg
    21841 cggcggtcgc gctgaagctg atcttcaccg tcccaggcgg cgcagcctgc gatcacctcg
    21901 acgagctgcc aggcggcgac caagtcgtgc aacgcctcga gaactatgtc gagtcgcacg
    21961 tcgtcgaggc gggcctcgtc cgcgaacagc gtcacgaggt gagcggctgc cgcgcagcgc
    22021 tcagcgcgct cgaccagacg atgacgcatg gccgcttcac gcgcatgacg gagtgcatcg
    22081 agaaggcgaa gaccgccgcc gacgcatcac gctgcatcta gcagccacac gcgatctcgc
    22141 ggcggccgct aaccgatttc agcgatcact tggtcgaggt cggcggggac cagacgcgag
    22201 ttcgtgcgaa ggccctcgac gaggtcgtct ttgctgccgc ccacgttctc gagattctcc
    22261 gcgacgcact tggggtgcag gtagcccgca ccgcgcgtca ccatcgcgcc ggtatcgatc
    22321 tcgcgctcga cggcgacgcg gagcgtgtcc ttcgcgatcg gctcttcgca ctgcatgcac
    22381 ttcgcgcggc cggtcggcgc cttgtcggcg tagggaaagc cgcctggctt cgcgcgacct
    22441 ttcgacatcg cgtcttgcat cgccttgtcg agctcggcgc ggttcgggac tggatcggaa
    22501 taggtgtcga gcacggcctt gagctcggcg ggcatcttct ctgcggcgca gaggaggtgg
    22561 tgccagcgga tgctgggcgt gtcaccgaac gcgttgggcg cttcctcacc aaaccgcagc
    22621 tcgcccttcg cgatcgtctt cttgcaggtg cggcagcttg cacggccgga cttcgcttct
    22681 tcgatcacgt tcgccatggc ggcgaaccta ctccaagtcg gatatgttcg cgccagtgga
    22741 acagccatac ccggtcatcg cgacgaagat cgacccgcgc agccccgagt accgcgccaa
    22801 ccacgacgcg aataccgcgg ccgtcgcgaa gctgacgaag gc
//
LOCUS       DATLBZ010000526        48247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8542313, whole genome shotgun sequence.
ACCESSION   DATLBZ010000526 DATLBZ010000000
VERSION     DATLBZ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 48247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48247
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8542313"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VMZ53_26265"
     CDS             <1..739
                     /locus_tag="VMZ53_26265"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HUS32044.1"
                     /translation="SELGMKIRKGFISAPGKVLIAADYSQIELRILAHLSGDPVLSSA
                     FRDRVDVHTQTAALVFDVPRESVTQEQRRIAKAVNYGLSYGQSDFGLARALDVPRTQA
                     AEYSRRYFQRFPSIHKFMDEIVAVARAQGGARTVLGRWRPIPNLMSKSPAARHAAERV
                     AQNTPMQGTGADIIKLAMLRTSERIAKERWPVQMLLTVHDELVFESPPDIAEGVGAAL
                     KDEMENVYKLAVPLEVDIGIGGNWGDI"
     gene            complement(736..3954)
                     /locus_tag="VMZ53_26270"
     CDS             complement(736..3954)
                     /locus_tag="VMZ53_26270"
                     /inference="COORDINATES: protein motif:HMM:NF019271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EGF domain-containing protein"
                     /protein_id="HUS32045.1"
                     /translation="MGKLRWAVLFVVFAACSGGSETAPRILSFTTFPSVVIADVPTSF
                     TFTFVLDSNDQVPVCQIDGVGDVTSGSQTMLTLSSDTTFTLHCMNGDGDATATSIVTV
                     TALPIAPALATFTANPTTVVVGTPTDVTFSWTFATTATPAPDCTIDQGVGAITSGETR
                     SVTQTSTRTYTLTCTNAGGSDSRQLTIDTAGAPVAPTIGTFTATPASATNGVPTNITW
                     TWTYSNSPTPAPTCSINNSVGTVTSGAMTSVTLTTATTYTLTCTNGGGTGTKQATVSV
                     AAVPVAPALATFTATPSTVTSGVATNVTWQWTYSNAPTPTPSCTVDNGVGTIGSGTVT
                     TVNITQNTTYTLTCMNSAGMATRMTSITVTPPVAPAIATFTATPSSVTSGLSTSVTFN
                     WTYANSPAPAPTCSINNGIGAIASGSSAFVNLTADTTYTLTCMNAAGTDTKQLTITVT
                     PPVAPIIGTFTATPNYVASGTNTYVGWSWTYANTPSPPPTCGLDHALGGVVNGQVLGI
                     TITTDTTYTLTCQNSAGQNSAQVTVKISTPPVLAGFTVTPSTVSVNTPTTATWNWSYS
                     NTPVPTPTCMITPGVSVTTPGGTSSITLSRARTYRLRCGNQGGIVVADVTVGVDECAA
                     NLDDCGANTDCIDTAESFSCACSTGYTGDGTTCSKLQACNTTPSLCDPHATCTNTANG
                     YACTCNTGYVGDGATCQRYRYEFVTSTTGTGNLATWAGAGGQTGIAAADAICSARAAA
                     AGLPGTYVAWISDAVNDAYCRVQGYAGKKSAVCGLSNGTLPVAAGPWARRDGVRWAGT
                     IDRLVSPYSELYSQGNLDENGVAVPTTDLVFTGTGADGAFKASGAADSCSSWTSASTS
                     DAAITGQAGAGSGYFSDQLAATNCDQLARLRCLEVGTGPAFPPHQVLPKRVFATSVSG
                     NGILGAWPDIAGLGLTGAAAADAICQARARYIGVANAASFRAFVSTSSSTAASRITGN
                     GPWFKLDGLPVAFSKTDLLDGQLFSPINRTELLGTPTSGFNGAWTGSTTAGINGSYTC
                     GNWTVGVGGTYGGTGKHDSFTSWVQSGITSCNTSEVLYCFEN"
     gene            complement(3978..7109)
                     /locus_tag="VMZ53_26275"
     CDS             complement(3978..7109)
                     /locus_tag="VMZ53_26275"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUS32046.1"
                     /translation="MTPPDDGPWDDATQHDDSAQMDGDDQPTMRANDDHVAALRKERM
                     KQRLIDDAASNVRRRTTNQGLQDRTRTPAQGNPTTPPHGVSTAQLDMHVSRTVTDRGI
                     PVQPDPSSTNRGIPVAPRGPMTASPDLASTSSPDIASALYAAAGDVEIDPSSTARGMP
                     AQGRTPAIAKGRHPTEDGVILLDRVSGNAPTDDAPRRSESASGGVVVVATGSEADRVR
                     RLCHTHSLLVPVMSSLAIAHEALYVIVIGEPSPPAPERVAHVVRPSVTDRQLVDLIRS
                     LVSGRVVAALPEASAEVDPRVLDAAQRIAKLDDRAAIESIMVDTITAVVGADRAHCLY
                     FDPLSGGLWSEGARVTLGDSRRAIAGFVGWAAYTGEALHAEPAGDDPRYLQELDDPEG
                     KAQTRLIVQPIIGGDRRTHAVLVAVRRWRHDPFKPSHAAALASLAALVAPTLDLVAPA
                     PPVADGRKPRTTIPGALKNAQTAALIAPPRTKPPTGPSPLTAPRPRPPSAPGMKRPPT
                     SPPTRDSSAGIDGGRSSSSSIDAPTAPRAPIVDEPADASDSKRLRTTSQPPSTSTTGR
                     TRAPTGESRPNALTPATASPTASRPRNPTDETRPEMPSGGAPRSPTPPGGSSRASTPG
                     GGSPSSARPRNPTDETRPEMAPGSSRPPTGPNPIAAHRPGTGPNPIAGHRPPTGPNPI
                     TGKRPPTGPNPVTGHRPGSGQSAGAPSTGPIPRPSAPLVEKLPLPAPRHPPSSPPAPS
                     ATIAMAHRVAIVAGDEEHDRARRIAKTCGLEIALASTTESAPADARLVTLGLPWSPDI
                     DPRVVYVARTTMSNDHLADLLTAVATGKALSSNESLGKANNPTEARRAQIAFTGSRAF
                     AGAVDFVSAETTAVSTLRELLDADRAYFLLYDDASGALSSPGRKLSMGDNRRSIAGLA
                     GWVAHTGRAALAERAASDPRWLGPIDDPDGDSNSQLLMQPVLRSDQHVQAIIVAARRP
                     RRPGFTDIDAALAERFASLVAPLLQQLHQHVENQKLIGDPTGAGSGDTKPAPAPTSPL
                     KSLLLRIKKLFGA"
     gene            complement(7112..7684)
                     /locus_tag="VMZ53_26280"
     CDS             complement(7112..7684)
                     /locus_tag="VMZ53_26280"
                     /inference="COORDINATES: protein motif:HMM:NF016973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HUS32047.1"
                     /translation="MSMDGELWHIVEAKRDDGTPTMFRIRELDPKKDLTRIFVVELPY
                     STHEMSRLPNAAAYRRVADLEEQWLRPACQTLGWEIVGTKTEDGSFFLYMYGGGDPQQ
                     LIEKVSPFDAALGFYDDEDPDWAEYATLRELLDQAKAIPKIAAKPAARKKPAAKKKTA
                     AKKKPAAKKPAAKKKKPAAKKSPASKKKRK"
     gene            7752..9038
                     /locus_tag="VMZ53_26285"
     CDS             7752..9038
                     /locus_tag="VMZ53_26285"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HUS32048.1"
                     /translation="MATACLKIFAMRWVVAAALALVACRGSTSDGQQPSRDLPPTPNA
                     AAATTPAKTHVEGSSTMRPHHEEPVIECSVHVPDIADDETRVQALLDQANREIERGSY
                     GAAWTCADRAADLAPTSVEAHHLRGASLAALNRDSEAQLAYSLALALDPDDPETLRAV
                     ADFYINGKGDRGRDALRLGLELAQRGSRRALARRRRNAPLAADLAVLEAQALNDLGRS
                     DEALERVDAAVRIAPNRGDAMHERGVALFDLSRFADAKVAFSKALQSQPQDAYTHQML
                     GLTLEQLGEFVAADTEQKKALELAPHELTPPVLIPVDEFQKEIDSVVATLPADRRAHV
                     DKIRVEIADLPDPDDLKAVQPPFPPTILGLYRGPVGRAPTASDEAPSIVLYRKNLARA
                     VKTRVELSEQIRDTLLHEIGHLEGLDEDDLRRRGME"
     gene            9087..10931
                     /locus_tag="VMZ53_26290"
     CDS             9087..10931
                     /locus_tag="VMZ53_26290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUS32049.1"
                     /translation="MSSDGTGLAASVPDSVQTLSSALRREQKKVALVQEVSKALSTVG
                     DIDALLRLIMSKVTELMEADRGTLFLVTEDGRQLWSKVSQGSEVVEIRLDIGEGIAGW
                     VAQTREIVNIPDAYADQRFQPAVDLKSGYRTRSILSVPMLGALGGLVGVIQLLNKSDG
                     PFTLADEELASALSSQAAIAIENARLYHSLVTQNQELSRARRDLERRQRELNALYEVE
                     KELSAALDLDDLLSRILAQAITVLGGGAGSIALTEADGSLRFRTVQGPAAPRLIERTL
                     PHGTGLLGWSIAHKTPIIVDDPANDHRHAHEVAKEAGVRPQHIIIAPLVDGEDVIGGI
                     EIIDQRRTVRDGENAWGEEDLKLLVLIAAQAASAIGLARRRSEQSNRDRLASIGRMLA
                     GLLHDLKTPMTIISGYAQLMAACDEPPQRETYVEQIQRQFDLMAGMTREVLAFARGDA
                     DLVVRKVYVNRYTEELATQLGAAIAGRSIEFMVEARYDGIAYFDEQKFLRVFHNLTRN
                     AAEAMPDGGKLRVIVERSVTSSCGPSRIPARASRPSCAAASSSYSRRAKRAAPASASR
                     SSRRSSTITTAWSSANRVAPAPRSSSSCRSPARPTAISARRIGRESRR"
     gene            complement(10894..11292)
                     /locus_tag="VMZ53_26295"
     CDS             complement(10894..11292)
                     /locus_tag="VMZ53_26295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32050.1"
                     /translation="MDSSQLLLERLTLLRDSWPNPPWTWDSRFQTVAASFSKEMEPDA
                     RKSAMNAFPRGWTTKSLENAPPDMQAIAERTGGLRSGQRLLAGDEMFAPKLFALWWPW
                     GGGDTITLRIGVDGATPELIAAIRDLFGAH"
     gene            11438..12090
                     /locus_tag="VMZ53_26300"
                     /pseudo
     CDS             11438..12090
                     /locus_tag="VMZ53_26300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            12087..14303
                     /locus_tag="VMZ53_26305"
     CDS             12087..14303
                     /locus_tag="VMZ53_26305"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HUS32051.1"
                     /translation="MSAARSAYWVWRRELQVTLRAPIVYIIGGLFLIVQGVAFAGLVT
                     VLSDPKKPAPLGALLEGQLAGTLLTWVLQLVVLTLLGMRTIADDKKTGAWELLLTARV
                     GEGAAVVGKWLGAVTIYALLWLPTLAYLGIVAIFRVDSGGWDAPSIITGYLGAIMLGA
                     ALLAWTVAASASTSNMLVAGALGFGMLIVFFLIGELPAAFPDLAIDHPTLARALEALS
                     LRGELTTFARGELSLVGVLYIVGLALVGLSLAIALACGGRRQRREVRTRVLATALIAI
                     IVVLGGVVALRRPVSLDVSAARRNSLDPETRLVLAELPGRAKLTIVRPTLSALEPIYD
                     EVERVAARMAASGAVDVRSYDPATLPGGIAAAARAAGVAPGDLESNGGVVVEVGARRR
                     VVDRLQLVAIGVNASGTPVVEQLEIEQAIAGAFAELARNQPLNVCATTGHGELPLVHD
                     PKDADWSAVVERLRGEGMTVAPVQIGAGVPEHCKVLVVAGPTTPLSSAEAIGVQTFLQ
                     RGGSLVVAAASRPVVASVAAAGQETTLASTGLEAVLGTVKLGLPPAIAVDPTLTVREL
                     PGALLVIDGYAAHPINDGFPQARATVWFQPRVVLATGKAQPLVRASTASWGERDLVSA
                     PPQKNEDDVGGPAVLAAISENGHVIAIGSAESFSTAVLKGGLSASDRWLAHAIRFASG
                     TPLPKVTLGSRGLDQVRLVLTPAQRRIVTWLSITGIPLVWTLFGALVIVLRRRKNT"
     gene            14300..15757
                     /locus_tag="VMZ53_26310"
     CDS             14300..15757
                     /locus_tag="VMZ53_26310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32052.1"
                     /translation="MIATTRGLIVLLVLAVVFGVLVIVVGPRKRESLDRALMPRFDET
                     RVNELVYTCPEGAETAIRDGKSWRLKTGVADAATIDATIAALRGARWHRSAEMTRAGK
                     TSCTLTVGDAIFAIGEKLEGTEQTWILRGRRAYLVDGWVANALAPGRLALHVRRPLAD
                     AKVDPHSHGIWIDPAVVQDIRATLDALELVSLDGKEGTPGAKAFGAEQLGSCDQNRVY
                     IRAPSGVGCVEAAAWQAIVDAEAKLAGPPEAIADRRPVPITPVKLVVSDRHDITLAGK
                     PQIDGVVDADAERVRELLTALATPGELVPVPETAPTDALLAVAADGTQVQLHMFGNGV
                     LYRRGEPIAIKTPSERLIARPSTDYRDPTRWREDPTTITSIKIDNVTFERGAVLGEWT
                     RKPPAKPGATVDTALVDALAQTLAVVRAPSNAGNGKTTNGRTLKVTFTPPAGDPSTHT
                     LVVSGAIRDGCRGRADNSEVIFPPELCTAVAALRP"
     gene            15778..16497
                     /locus_tag="VMZ53_26315"
     CDS             15778..16497
                     /locus_tag="VMZ53_26315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32053.1"
                     /translation="MWRVAGLVLLAACGRGFFNPDLSPDPDAATPVTAPSYVQSNYAT
                     SSMGQVTAIDAAFPLSQTAGNTIVVVVMWDSTNAALLTLFDRAGNTYRSAAGPTLTNG
                     NWTQTSFIATDIAGGAAGTNVVEADLNASSPMRIAVLEYANVSTAQPLDGNAQAKGDS
                     GMLPAISITTANPNELLVVAASTDYMFTDVFPTPATRVSGTNFFVQDKAAPVPATHVF
                     ATPNGTSNGNWVAQVLALQGR"
     gene            16688..17017
                     /locus_tag="VMZ53_26320"
     CDS             16688..17017
                     /locus_tag="VMZ53_26320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32054.1"
                     /translation="MSDFETLTDDQLSECSGGYGAQLNSAMHRARELGLRITSTTGGR
                     HAPHSYHYSGRAADVAGPASAMRQFYREMSRTHPTELFYDPMGGIKHGRQIGPIGGHR
                     THVHVAY"
     gene            complement(17044..17793)
                     /locus_tag="VMZ53_26325"
     CDS             complement(17044..17793)
                     /locus_tag="VMZ53_26325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32055.1"
                     /translation="MSHRLGVFATAFTLPVVLLAGAACDKDKLKGPEAEVKKTDVKLD
                     LPAVPAFDLPPAPADGSHTVKELRVKGKKLLDTDLTVHGFVTWAYDCKTAIRKGEEDL
                     KDVQARIDEDPTLCERPKFYIGDTAQTPVEKSLWVVDVPRPYNALEVKRIKKKDRVEP
                     DRCEPDEKDKSKSICPPYAVGDEVVITGTFKTSSPHSERNSDGLIVYKSMKNVTQAYE
                     SPAPVEMPGATGAGAPPPGALPSPEDLVKKK"
     gene            17809..19503
                     /locus_tag="VMZ53_26330"
     CDS             17809..19503
                     /locus_tag="VMZ53_26330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS32056.1"
                     /translation="MVTKGSQVRVVLAIAATICACESPPRVKPWRHAPDPTAEAANAP
                     GSPVLAQAAPSEAQVIANRSHTLRIHMDADPGRLTPIVEPSIWAKRITVGTIFEPLLR
                     YEPADANGPARFTGRLARSWQLMPVPGGIVIRIELQPGVTFHDGTPLTSSDVQWTLDT
                     IRDGRRHDLEHLKPMLDDVFRIELLTPLELQLVLLRPNAWVLRALAEIPILPMHVYDG
                     SLLAGGALVGTGPWKMGSHKGGVVHLVTNDKYWAGKPAISDVEFVYEPDAAAALTDAK
                     RGELDIVPALIPAHWPEQASAPGLQASFSALQLAPPRLRMFRFNASRPSVADPRVRHA
                     LALLIDRRAIAKRVFDGLARPANWPIWPGGPVDGAEVAAPDFDPAAAAKLLDEAGWVD
                     TDKDGLRDQGPDKQLKLVLIGRDKPAPKDSSGPPVKTERDYFVDAARRAGVIIEVRSG
                     GDSWLDKHIEGGDWDLLEVAWSGRVDMDITPLVGGRDATRPQQPHVDRVLDAMTAAWD
                     PAERGKLAKELAVALAESWPVAGITADAPQGLIHHRVENVKVWDGWLDLSQLRFAK"
     gene            complement(19785..20492)
                     /locus_tag="VMZ53_26335"
     CDS             complement(19785..20492)
                     /locus_tag="VMZ53_26335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32057.1"
                     /translation="MKAILVAVVGTAGLLATPAVAGNQKKQTSAKAPAKAEIAKPAVV
                     VVADASAEAGAGAALAIADVIAQPAAATEMLALPELPADTAGVFGQVSLPAPVLAAKP
                     IAKPVKEKAPVMKMGNDYVLGGGSKRTSAKPTEEVQQFVPKGLSRAQVSTYIDSHADE
                     IQLCWSRIPAKQRPDACTVDLDLTINDAGQVTDVELGGDVPAGAHKCIVHAVSHWSFP
                     TAETSTQIEYGISLHSI"
     gene            complement(20552..21742)
                     /locus_tag="VMZ53_26340"
                     /pseudo
     CDS             complement(20552..21742)
                     /locus_tag="VMZ53_26340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            21935..22177
                     /locus_tag="VMZ53_26345"
     CDS             21935..22177
                     /locus_tag="VMZ53_26345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32058.1"
                     /translation="MRFISIVGAIGLSACVTHVSRLGPFVKNLSVQPQGIVVDSCVVQ
                     YETTTDYTPASGGNRKDHELVEGTCYRDVVPTGGVR"
     gene            22174..22410
                     /locus_tag="VMZ53_26350"
     CDS             22174..22410
                     /locus_tag="VMZ53_26350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32059.1"
                     /translation="MRALVVCAALLAGCVNHSQVGPYVKSVSRADNSLMVIRCMIELV
                     GEDLREGTCERLYVPLGSMPLVPPPPMQPPPQMK"
     gene            complement(22398..22706)
                     /locus_tag="VMZ53_26355"
     CDS             complement(22398..22706)
                     /locus_tag="VMZ53_26355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32060.1"
                     /translation="MGTLRTSEEGAIAAGEGRKSENLDDNRRVDEAPKIIISAGPIAE
                     GATTHTPAPARAVPAAAQRRTGSLPAAPVKQGGIGLVVLLYLLCAAALGYAIYERYFI
                     "
     gene            22734..24122
                     /locus_tag="VMZ53_26360"
     CDS             22734..24122
                     /locus_tag="VMZ53_26360"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS32061.1"
                     /translation="MAHLLLIDDDARGEAVVKALTDQGHEVRWARSMADGLRQFRDDR
                     ADVVLVDTDLPDGSGHDVLRTVRIEDPTTMVIVTSAKGTIEEAVDAMKQGASDFVRKP
                     LDLPDLALRVSKSLSSRRLVTQLEYLKAQQARGSMISGLVGECPEMRDVFGTVIRLGQ
                     KTGRRAGPTVLIAGETGTGKGVMARALHYNSSRRDGPFVEVNCASIPENLMEAEVFGA
                     ERGAYTGAVAAHVGYVETAEGGTLFLDEIGCLSMPLQAKLLTVLESRTYRRIGSSVER
                     RADIQTVVATNMDLHAAVDRGAFREDLLHRLEIIVLKMPPLRDRGPDRLRLAAHLRDE
                     ICRDYGLAPRPFSEDVKDLIEGYHWPGNVRELRNALEQILLLEDAPMISARHFMLRGR
                     RQTPTKLPRLGTDNGSVELDVPEEGLALQAVTDALIDRTLAMCEGNVSRAARMLGVSR
                     DQLRYRLAKRGA"
     gene            24303..25778
                     /locus_tag="VMZ53_26365"
     CDS             24303..25778
                     /locus_tag="VMZ53_26365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32062.1"
                     /translation="MLVIEAEQGERLRLGHVLGNYRLIRKLGEGGVGTVYEGEHIRLG
                     RKMALKVLHPETASAEVIVRFFNEARAVNQIRHPNIVDVEDFVTAETGEHYLIMELLA
                     GEDLRTIATREGVLDPRRVAAIGEQIASALGAIHGAGIVHRDIKPDNIYVCRKPDGTE
                     IAKLIDFGIAKFLGSAEGMTREGMTLGTPEYMAPEQILSSGKPGPAADLYSLGMLMYE
                     CVSGAPAFTATTTAAILRGHISEPVVPPSLRRGEPVPPVLEAAILKCLEKDPKNRFAS
                     GDELATALRAERAVDLITPRFEPGKPRRKSRALQMLPAFAMAAAAAVLHFLPKPENQA
                     HAEVAAAPTPAPVAVVEKPAEAAPAPTPTPPPPAAPKAMDVALTSSPDGAELYMGEEP
                     LGPAPQIVSIPISSTAVELTARFDDGVEVVQTIVPDRPLPQIAFVKPAAPKPSQAVAV
                     KKTTRVTRPTKAAATKAAPTTTTGAGTMTGDKRDGTMDPFK"
     gene            25775..27286
                     /locus_tag="VMZ53_26370"
     CDS             25775..27286
                     /locus_tag="VMZ53_26370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32063.1"
                     /translation="MKSCPSCRRIYVDSEQVCPLDDGRLVASNAPAPAGVGRAMGPYL
                     LVSQLGEGGMGTIYIGRHTGLGRYVAIKVLRPELASRKDNVARFFQEAHTINRLKHPN
                     IVESIDLVEDAIDGAYCVLELLRGPDLKTRLARGALSLESAVHIGVQLADALGAVHAL
                     GVVHRDLKPENLILIARDGRDDTVKLIDFGVAQIGGEGTQAVIGTAAYMAPEQAVTGA
                     SVDRRADVYSLGVLLFEMVTGRHPFPSATDSEYILRHAEDKPPRPSKLASKCPPALDD
                     IILRCLAKKPEQRYADAAQVASALRLVNARAGRSKAGVIIAAAFVIGGGAAAAYLIVP
                     RYLQKNSEAKAETVAEAPVVENAPVEKPVEKPAEQVAEQAPVEAQPAAPTIVEISIHS
                     KPAGATVFRAGETVALGVTPFTVQLPRSDTPTHMRFELGGHNAKIIEVPIAEDMEINV
                     NLAKEATSEAVHAVRLKKSTKSSKSSTGEGSPPPVVPSDKKVQREGVIDPFAK"
     gene            27301..28482
                     /locus_tag="VMZ53_26375"
     CDS             27301..28482
                     /locus_tag="VMZ53_26375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32064.1"
                     /translation="MRLALITFAIALMGSTPAIADDVVILPPLGSAKDAKPVSTALAG
                     SKVGTLATLKVDAACTEDPRCLATKGSELSANRIVAITVTASGKLDMVVVDVGAKLLL
                     GTRSIAVAPKKMGKELGPAIARAIEDMTVDKAKALFAEGNESYNLGEFERALDRYKLA
                     YRVKPLPAFQFNIAQCHRKLGQNKEAIAMYQAYLVGVPNAPNKDVVESLIAESKKAID
                     EQVAAQRKLETDKLDVEKKKAEEARKAKEAEAAAAAERAKAEQAQIAAERERAKGYNR
                     HPARKFMVVTSVLGLATVGAGAYFGIQARDAQNNFDNNQCGDPTVPRLQPLIDQCNAD
                     KDKGTRDALLSNVLIGSGGAVVLVSALIYALDPGNVEAPKEKRVGLAVSPTSVQVVLK
                     W"
     gene            28482..32429
                     /locus_tag="VMZ53_26380"
     CDS             28482..32429
                     /locus_tag="VMZ53_26380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32065.1"
                     /translation="MRILIACVVLLGACKFNEQDFDDRACSADSECRPDEGCAANVCT
                     QKGCSVATDCGINGKFVCQENACVAAACSSDSECGTGFACSESFCRPSFNIAAAESMS
                     NTSISVTFDAPPDPTSAAVLANYSVSGLSLSGTPALVGNTVTLTTSTQTATSYTVTVS
                     GITRALDKAPLIGVSATFTGRAAFNVTTASATTSRTVILTFDGMPNVTEAATLANYSI
                     AGLSLSGTPLVVGNTVTLLTSSQAATSYTVVVNGVHRAGDAEGLADADAVFTGRDDFN
                     VLSAASESSHTFTVTFDAPADDLKAKTLANYSVVSSGNVPLALSGVPQLSVDGTTVTI
                     TSAAQAAGTYIVTVNNVTRAADNEPLNVKTANFTGRIPFNVFSAMSTSSTTVTVTYDA
                     TPVMADAIDIANYDIPGLTVNSATISGNTVTLATSPQSGTSYTVTVSGVARNSDGEPL
                     TVAAATFTGRAPFNVAGASTITSHSTTVTFDAPPNASQATVASNYSIPGLTVITAAYP
                     GTGNVVTLTTSAQSATPFQVTVNGITRASDGEALTLAVASFTGRSPFNVSSALSVTST
                     TVTLTFDAPPNATQAQQLGNYTANNGLTFSSVSYTGTGNTVTLTTNAQAATMYQVSVA
                     GITRASDGEPLTVTSATFTGRTPFDVAAAASTGNTTITVTFSAPPNATQAQTIGNYQI
                     TTGSLSLSGTPVLNGNTVTLTTSVQTGGAAYAVTVSNVTRASDGEALTTATRSFTGKS
                     GFNVASASSVSTTSLSVTFDAAPDMTTATNVNNYTVPGLTLSGPVTLSGNTVTMTTTA
                     QAGQTYTVTVSNVVRASDAVALTVNSTSFTHTTFNVASAAAVTSHSITVTFDALPNTA
                     QATTLTNYVVKDQMNNTLALSGTPTLNASTNTVTISTTTQNGTKNYTVTVSNVTRASD
                     GTTLSTKTAQFAGIGSFDVASATAPTSASISVTFNTPPNVTQATTLANYAVSGLTLKG
                     TPVLSGNTVTIATSAQMATTYTVTVSNVTRASDGEPLSNTSAMFTGKAPTAPTVTNVV
                     VTGTNPNNNTTFYNTGTATVVITGTQFTSVVCPAGVTLDDTNGVAASVKTPAQSCTVD
                     NDTQITAVFPAGIRTNGSTGWNVLVTNTGGTNAASSVKLVVKAGLLISEVLVGLSSGN
                     NTREFFEIYNPTATAIDTGSIGLVVHFRASNGTDTMPALSFLSPANQHRTLASHGFML
                     VSSSTSVADTWYSHRDATYDPVTTELAGNGGIYLSLSTTAQAKVIDKLGWGTQVAGGF
                     EGTAFTNISNNNSAQRKPAGGAGNATDTDVNLADFNTQTPAITPKGTADGVEP"
     gene            complement(32436..32783)
                     /locus_tag="VMZ53_26385"
     CDS             complement(32436..32783)
                     /locus_tag="VMZ53_26385"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32066.1"
                     /translation="MKAKLISLLLAGAAAVTVPTGCTGTTHGYVVASYDAPPAPREEV
                     VYFRPGYVWVHGNWVRDRYNGWRWQNGYYVRERAGYVYDNGRWDRRGNQYIWVQGTWR
                     PRNTVVIRGHRRW"
     gene            32973..33971
                     /locus_tag="VMZ53_26390"
     CDS             32973..33971
                     /locus_tag="VMZ53_26390"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HUS32067.1"
                     /translation="MTRVAVFGAGAIGCWVGGRLSAGGCDVTLIGRSRVLDELRAGLV
                     TSELHGGTRTAKPALSTDSSVIGNADLVLVTVKSAATPDAAREIGPKLGPNTIVISLQ
                     NGVRNVPVLRDALPGRTVIAGMVPFNVIRQRAGQYHRASGGKVMFDASDRAAAFSEAC
                     LRADLPFELRDDMPGVQWAKLVMNLNNAINALSGLPLATELSTRAFRRCLALAQREAI
                     GMIRAAGEPLARVTLVPTRWMPGLLTLPDALFKRLAGRVVAIDPTARSSMWDDLEAKR
                     PTEIDYINGEIVRLAEKLGRTAPMNGKLVQLIRAAEQGGKRDFSGDELFRAMQPTA"
     gene            complement(33952..34518)
                     /locus_tag="VMZ53_26395"
     CDS             complement(33952..34518)
                     /locus_tag="VMZ53_26395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32068.1"
                     /translation="MAVVDLPPGIGLVGTPLGSFEAPKTARRLATASLLIVIFCGALT
                     WVFVAKHWWRWHASTQFVLPIAFFAFSALGFAVRRAKVAVTRDGVRWGWTSLSFTQEA
                     ARITTCHVYRDGVALEAKRGSWWFLGARDWDRFDVLVRQLKRAELPVVEHEGKAPFRA
                     RLQSYGRFLDGLLVGSMVSAFIVMLWAA"
     gene            complement(34518..37148)
                     /gene="glnD"
                     /locus_tag="VMZ53_26400"
     CDS             complement(34518..37148)
                     /gene="glnD"
                     /locus_tag="VMZ53_26400"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HUS32069.1"
                     /translation="MFATLPPRLKAVGSGSAACRTIADVYDHALATRFEQACAGISTP
                     LALVATGGWSRRELAPYSDIDFIILYERDEASAKQVSDRLLYPLWDEKLAIGHALREA
                     RAVARLARDDLATATALLDARHIAGDRRLTTDLLRATLSALAPGGNPNEVIAMLAAEK
                     KARHDRFGASLYLLEPNLKQGIGALRDLATALWAAQVRWHPPRPGVDPEGADSLIASL
                     VAMGHLTRRQGDVLTTARDFMLRVRTLVQLTAKRRFDQLTFEIQEAIAPSLYPDAKAH
                     EGDIRSAVAPAVEALMRDYYLHARAVVQVADRLLEAARVPARRKPRIAQVDASFITFN
                     GELAIRDPRLFVDRPSEMVRMFRVAVDEKLPVYGHTKELVAETIARDPATLAADPVAN
                     KLFLDALVDPRDATQPSAFEMMHQLGILSALMPEWTPCTGRVQHDLYHVYTVDQHQLY
                     ALAMQKRLARGELAEEHPLATELWREVKRPAALFLATLLHDVGKPLGKGHAEKGAVVA
                     AAIARRLGLSDEEMEVSEFLVRQHLTMSHLSQRRDLSDPDVIARFAERVGSDDRLVKL
                     YLLTLCDTAMTAPDNLTTWKAGLLRDLMLRTRAYFRGESQAGEGESEDRSIRNKIVQL
                     VEVPDARPLVDGIDPRLVAQLTARQAARHIKLVHGTQSKQPPIAFEVHCFPMKGHSEL
                     AIVAPDEPGVLAAIAGALTANRVDVLGAVLGHVETTGRRLVMDTFYVRDLKGEAITDD
                     DPRWPRLLTDLRELLAGVPDPGAAAALIARRRPKSGLPKRVTPGVTTEIKLHDDSTAA
                     TIVEVFTLDRVGVLYAITQTLADLGFDISLAKVSTEGEKVADVFYVTRGGKRITDEKE
                     RAVLLQRLRLAVEAPGLTGS"
     gene            37309..38745
                     /locus_tag="VMZ53_26405"
     CDS             37309..38745
                     /locus_tag="VMZ53_26405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32070.1"
                     /translation="MLGRYQVLRPLAKGGMAEVLLARSIGIKGFERFVVLKRILLQHE
                     NEHSIEMFLDEARLVAGLHHRNIVQVHDIGEEEGKYFFAMEYVHGEDVRALLRAAKDK
                     KQQIPIEHVIAIVTAAATGLHYAHDQKGPDKKPLGIVHRDISPGNILIGFDGSVKVVD
                     FGIAKTESQHENTQAGEMKGKIGYMSPEQCQAKDIDRRTDVFMLGIVLFELCTVRRLF
                     KGETKFDTMQSIVSGNVPPPSKFRKDLPEELEKIILKALATDPNDRYQTADDLRLVLE
                     AFAMRNQIQISGTRLSDYMKELFGERPLPWLVEELPPMPDLQMVRARDSSPVVARAAS
                     LTESPSGNVAENPSESTPMAWSTRPEAKGKPTWFWPVIGAATLAIVGVVALFTVNSKS
                     QPAANPKPIVEKPAAPTPPVATPDAGIEVVAAPVDAAVEVVETPDAAVEVATTPPKPP
                     KQKIRHPKPPAGPKPPSGGDDLDNPFPK"
     gene            38752..39711
                     /locus_tag="VMZ53_26410"
     CDS             38752..39711
                     /locus_tag="VMZ53_26410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32071.1"
                     /translation="MHVRLATILLASVAFVPLAAADDPFAQRDEGQAPQQPTSPAPPQ
                     AIPDPPKKPPTKQAKADAKKHITEGTKLYNTQQYAQAAEHYRAAYMLDSQPEYLYAAA
                     QSQRLAGDCTNALLSYNAYLRTNPKDAERDKAQKNIERCEQDLKDKDAAAQAEAARKA
                     QEEAAAKQAADAAAAKAAADAADAKRRADEAAANRAAEEAKAAAARHEAAKKSYVPGH
                     IMLGVGLVAVGGGIFLFRDAHSTIEDFNATASYDEFLAGQSAADDAHTRQTLGVLTIS
                     GGVALVAGAAVFYVLHSRSSTEVPAVSASITHDSAGLVFSGAF"
     gene            complement(39708..44390)
                     /locus_tag="VMZ53_26415"
     CDS             complement(39708..44390)
                     /locus_tag="VMZ53_26415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32072.1"
                     /translation="MGTITRALVLCVAVVLVGCAEPREAFQCATSDQCTHAGQVGTCE
                     STGFCSFDDRGCPSGKRYGESAGDDLSGECVVNPNGDFIVASAAAINHVTIAVSFSQA
                     PDSTEAVNLANYSVDGLTLVGPPTLTGSIVTIPTVEQADTTYTVTVSNISRASDDAPL
                     WMGSATFKGRPEFKVVSAASATNGLAQVTFSGPPDLATAMALSSYSIPGLTLSGTPIV
                     VGNTVTLVTSPQSATSYTVTVSNIVRAGDAEALTASSASFTGRLGFNVVSAAAPNSAT
                     VVVTFDAPPNATQAATLANFSIPGLTLSGVPVVAGNTVTLTTSPQASQTYTVTVSNVT
                     RGSDAEPLIIAARNFSGVNTFNVASAAPSTSNNVQVTFDAPPNATQAGTLANYSIPGL
                     APTAVSYPGTGSVVTLTVPAMAATTYTVTVNNVTRATDAEPLANKMATFTGRPKFNVV
                     SATSLSSTSMRITFDGPPSATNGVVLGNYSVNNGLALSGTPAISGNTVTITTATQSAV
                     TYTVTVSGVTRSGDNEPLLANSTTFTGRTSFNVVSASAISSTSVAVTFDGPPGAGATT
                     AANYSIPTLTVSNATYPGTGNVVTLTTAPQTGGMYTLTVNNVTRTGDLEALTAKSATF
                     NGRLPFNVTMAQATLSTQVQVTFSAAPNATQAGNVNNYFISGLAISAVSVTGSVATLT
                     TAAQANQSYTVVVSNVTRGIDGELLTTNSFTFTGRTPFNVSSATPVTSTSVAVTFSGP
                     PGAGATTAANYSIPGLAVSAAAYPGTGNVVTLTTAAQSSTSYTLTVDNVTRTGDVEPL
                     TNKTATFTGRLPFNVTGAAATLNTRITVTFDGTPVSGMANTASNYVLSGGLAVTSVNY
                     PGTGNTVTVNTSAQTGGTNYTVTVNNVTRTDGEALTVKTANFTGRSAFNVTGATSVGN
                     TSLSVTFSATPGTGATTLGNYMITAGSLSLSGTPVVSGNTVTLTTSTQSAGSSYTISV
                     ANVLRSGDNEPLTNASATFTGKAGFNVASAASVNHNTMSVTFDATPVITEAQTEANYT
                     FSGGLTTNGAPVLVGNTVTIPTSTQAGQLYTVTVNNVHRAGDNFPLSVNAANFTHTLF
                     NVTSAVSTSTHSVDVTFDAPPNSGQATTMSNYAFSGGVVPTGTPVLNGSTVTIPTSDQ
                     VSGTLTVTVSNVTRGSDGTILSNNMATFAGRTPFTISAMSTSSGAMSVTYSDPPQVTP
                     ASTNSNYSVSGLTLSNPSLSGNTVSFTTTAQVATTYTVMVSNVLRASDNEPLTVNTAS
                     FAGTALSTPVVTNVVVQSTNVQGSGANGTTFYNVGTATVIITGSEFTGVSCPSGVTLD
                     DTNNIGTPVSTPATSCTVDSGTQITATFPSGIRSSYGGWNVKVTNAVGTNTTSTVKLV
                     VKAGLLVSEILTHAGGGNGADHEFLELYNPTASALNLSTLGVRLHVRTQGGTNTQLAL
                     TYTRTTIPSRGFFLITSTGSNNSDRWDGFQDAQYNSATADLVDNGGVYISLSTTANVR
                     PLDKVGWGTQVANGFEGTALPNISPTFSVSRKPNSSDASTDTDANATDFNPQTTIISP
                     KNSLGQAP"
     gene            complement(44430..44828)
                     /locus_tag="VMZ53_26420"
     CDS             complement(44430..44828)
                     /locus_tag="VMZ53_26420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32073.1"
                     /translation="MSLPRGLEDKIDQYVADALGATGTRAARASKPPPIQAAPIVDDV
                     DIDVSDMDVAAEDDILPVEIDDRTDPGSPNIFSFDEGTAWLAPAFSPPHDQIPTLSRI
                     RTARGSCPPPPEIDDDEVTNVVGIPPSPKP"
     gene            complement(44834..45934)
                     /locus_tag="VMZ53_26425"
     CDS             complement(44834..45934)
                     /locus_tag="VMZ53_26425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32074.1"
                     /translation="MRIVPSLFTLLAVTGCPGTAPPDQNATPDADVDADVTPTGLVLS
                     GKALDYFGAVALGDAALETDGLEPKATMTSATDGAYMVEVAVGSKFYVVGSKTGYRNT
                     RNTPITVSDMALSQDVYLMSVQDIQNQYTSINSPTPVAGTAFVTAELRRNNNTALEVP
                     LASVQLLDGNNVVVTGVKGPYAYNSAGNVDMAATTVAAFGGRARIAYFDVPPGTYTLS
                     VTYQNAQNQPVTNTTSLTTIADGATLALSGGMQGPGMGNGVTDPSFATHIYPKLQRAA
                     QGGLGCANCHTLSGPAAVLKYDDPAATVLANMKAATGVINTTTPADSLLLKRPLYEQP
                     PTAQDHPNATFIDVNDADYKLFLLWITNGAKP"
     gene            46093..47556
                     /gene="guaB"
                     /locus_tag="VMZ53_26430"
     CDS             46093..47556
                     /gene="guaB"
                     /locus_tag="VMZ53_26430"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HUS32075.1"
                     /translation="MLEEPLREALTFDDVLLVPSYSEVIPRDVDVTTRLTPLIPLRMP
                     LVSSAMDTVTESATAIRMAREGGMGFIHKNLSIEDQAREVHRVKKAQSGIVVDPLTIA
                     PSRTLEEALSIMRANRISGLPVVDADKRPVGILTNRDVRFEKRFDIQVGEVMTKNPVM
                     VKEGVGIDEAKDLLHKHRIEKLLVIDGQGALKGLITIRDIEQAQENPLAATDERGRLR
                     VGAAVGVGPDRDARIEALIAAGCDVICIDTAHGHSAGVLESVRQTRKKFPNVQLVAGN
                     VATGEATKALIETGVNAVKVGVGPGSICTTRIVAGVGVPQLTAIADAVAAAKGSGVAI
                     IADGGIKFSGDVAKALAAGADTVMIGSLFAGTDEAPGEVILYQGRSYKSYRGMGSIGA
                     MKEGSKDRYFQGDVESAAKLVPEGIEGRVPYKGSLRESIYQLIGGLRSSMGYLGCATI
                     AELHAKAKFVKISSAGLRESHVHDVIITKEAPNYRVE"
     gene            47556..>48247
                     /gene="guaA"
                     /locus_tag="VMZ53_26435"
     CDS             47556..>48247
                     /gene="guaA"
                     /locus_tag="VMZ53_26435"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HUS32076.1"
                     /translation="MHQLGKSHQLVLVLDFGSQYTQLIARRIREQSVYCEIHPCTMAA
                     DLEKVRAMKPLGIVLSGGPSSVYEEGAPSIPVGLYELGIPVLGICYGAQLTAHLLGGK
                     VHPAEKREYGRATVRVLQSPGAAKEGVFKTVNAGEELAVWASHGDHVEGLPSGFVHTA
                     ESDNCKFSGFAHHARKIHCIRFHAEVVHTPRGAELLGNFLFGVCGAAGDWSMASFVEE
                     AVEKIRAQVGSDG"
BASE COUNT         8146 a        15825 c        16297 g         7979 t
ORIGIN      
        1 ctcggagctg ggcatgaaga tccgcaaggg tttcatctcg gcaccgggca aggtgctgat
       61 cgcagcggac tactcgcaga tcgagctgcg catcctcgcg cacctctcgg gcgacccggt
      121 gctctcgtcg gcatttcgcg atcgcgtcga cgttcacacg cagactgcgg cgctcgtgtt
      181 cgacgttcca cgcgagtcgg tgacgcagga gcagcgtcgg atcgcgaagg ccgtgaacta
      241 cggtctgtcc tatggacaga gcgactttgg tctcgcccgc gcgctcgacg tgccgcgtac
      301 gcaagcggcg gagtactcgc gtcgctactt ccagcgcttc ccgtcgatcc acaagttcat
      361 ggacgagatc gtcgcggtcg cgcgcgcgca aggcggagct cgcacggtgc tcggtcgctg
      421 gcggccgatt ccgaacctca tgtcgaagag ccctgcggca aggcacgccg ccgagcgcgt
      481 cgcgcagaac acgccgatgc agggcaccgg cgctgacatc atcaagctgg cgatgctgcg
      541 gacgagcgaa cgcatcgcga aagaacgctg gccggtgcag atgctgctca ccgtgcacga
      601 cgagctcgtg ttcgagtcgc cgccggacat cgccgagggc gtcggtgcag cgctcaaaga
      661 cgagatggaa aacgtctaca agctcgcggt tccgctcgag gtcgatatcg gcatcggcgg
      721 caactggggc gatatctagt tctcgaagca gtacaggact tcgctcgtgt tgcagctcgt
      781 gatgcccgac tgcacccagc tcgtgaacga gtcgtgcttg cccgttccgc catatgtacc
      841 gccgacgccc acggtccaat tgccgcaggt gtacgaacca ttgatgcccg cggtggtcga
      901 tccggtccac gcaccgttga agcctgacgt cggcgtgccg agcagctcgg tgcggttgat
      961 cggtgagaac agctggccat cgagcaggtc cgtcttgctg aatgcgaccg ggagaccgtc
     1021 gagcttgaac cacggcccgt ttccggtgat gcgcgaggcg gcggtcgagg agctcgtcga
     1081 aacaaaggcg cggaagctcg ccgcgttcgc gacgccgatg taacgcgcgc gtgcctggca
     1141 gatcgcatcc gccgccgcgg cgcccgtgag gccaagcccc gcgatgtccg gccatgcgcc
     1201 gaggatgccg ttgccgctga cggacgtcgc gaacacgcgc ttgggcaaca cctgatgcgg
     1261 cgggaacgcc ggcccggttc cgacttcgag gcagcgcaga cgggcgagct ggtcgcagtt
     1321 ggtcgcggcg agctgatccg agaagtagcc gctgcccgcg cctgcttgcc cggtgatcgc
     1381 ggcgtcgctc gtgctcgccg aggtccagct cgagcacgag tccgcggcgc cggatgcctt
     1441 gaacgcgccg tccgcgcccg tgcccgtgaa cacgagatcc gtcgtcggaa ctgcgacgcc
     1501 gttctcgtcg agattgccct gcgagtagag ctcggagtac ggcgagacca agcgatcgat
     1561 cgtgccggcc caccgcacgc cgtcgcgccg cgcccacggt cccgccgcga cgggaagcgt
     1621 gccgttcgaa agcccgcaca ccgcactctt cttgccggcg tagccttgaa cgcggcagta
     1681 cgcatcattg acagcgtcgg agatccacgc gacgtacgtt cctggcagac ccgcagctgc
     1741 cgcgcgcgca ctgcagatcg catccgccgc agcgattcca gtctggcctc ccgcacctgc
     1801 ccacgttgcg aggttgccgg tgccagtcgt cgacgtcacg aactcgtagc gatagcgctg
     1861 acacgtcgcg ccgtcgccga cgtacccggt gttgcacgtg cacgcatagc cgttcgcggt
     1921 gttcgtgcac gtcgcatgcg gatcgcagag cgaaggcgtc gtgttgcacg cttggagctt
     1981 gctgcacgtc gtgccgtcgc cggtgtagcc agtcgagcac gcgcacgaga acgactccgc
     2041 cgtatcgatg caatcggtat tggcaccgca gtcgtcgagg ttcgccgcgc attcatcgac
     2101 gccgacggtc acatcggcta cgacgatgcc gccttggttt ccgcagcgca ggcggtacgt
     2161 ccgcgcgcgc gagagcgtga tcgacgacgt cccgccgggt gtcgtgacgc tgacgccggg
     2221 cgtgatcatg caggttggcg tgggcacggg cgtgttggag taagaccagt tccacgtcgc
     2281 ggtcgtcggc gtgttgacgc tcaccgtcga cggcgtgacg gtgaagcccg cgagcacggg
     2341 cggtgtcgag atcttgaccg tgacctgcgc gctgttctga ccggcggagt tctgacacgt
     2401 cagcgtgtac gtcgtgtctg tcgtgatcgt gatgcccagg acttggccat tcacgacgcc
     2461 gccgagtgca tggtcgagcc cgcaggtcgg cggcggcgat ggcgtgttcg cgtacgtcca
     2521 cgaccagccc acgtacgtgt tcgttcccga tgcgacgtag ttcggggtcg ccgtgaacgt
     2581 accgatgatc ggcgcgacgg gcggcgtgac cgtgatcgtg agctgcttcg tgtcggtgcc
     2641 cgcggcgttc atgcacgtga gcgtgtacgt cgtgtcggcg gtgagattca cgaaggcaga
     2701 cgagcccgac gcgatcgcgc cgatgccgtt gttgatgctg caggtcggcg ccggggccgg
     2761 cgaattcgcg tacgtccagt tgaacgtcac gctcgtcgag aggccgctgg tgacggaaga
     2821 gggtgtggcg gtgaacgtcg cgatcgccgg cgccacggga ggcgtgaccg tgatgctggt
     2881 catccgcgtg gccatgcctg cgctgttcat gcaggtgagc gtgtacgtcg tgttctgcgt
     2941 gatgttcacc gtcgtcacgg tgccgctgcc gatcgtgccg acgccattat cgacggtgca
     3001 gctcggcgtg ggcgtcgggg cgttcgagta ggtccactgc cacgtcacgt tcgttgcgac
     3061 gccgctcgtc accgtggacg gtgttgccgt gaacgtcgcg agcgcgggtg cgactgggac
     3121 cgcggcgacg ctgaccgtcg cctgcttggt tccagtgccg cccccgttcg tgcatgtgag
     3181 cgtgtacgtg gttgccgtcg tcagcgtgac gctcgtcatc gcccccgacg tcaccgtgcc
     3241 gacgctgttg ttgatggagc acgttggtgc cggcgtcggg ctgttggagt acgtccacgt
     3301 ccaagtgatg ttcgtcggaa cgccgttggt cgccgacgcg ggcgtcgcgg tgaacgtccc
     3361 gatcgtcggt gccaccggtg cgccggcggt gtcgatcgtc agctgccgcg agtcgcttcc
     3421 gcctgcgttc gtacatgtca gcgtgtacgt gcgcgtcgac gtctgcgtga cacttcgcgt
     3481 ctcgccgctc gtgatcgcgc cgacgccttg atcgatcgtg cagtcgggcg ccggcgtcgc
     3541 ggtggtcgcg aacgtccagc tgaacgtcac gtcggtcgga gtgccgacga cgacggtcgt
     3601 tggatttgca gtgaaggtcg cgagcgcagg agcgatgggc aacgcggtga cggtgacgat
     3661 gctggtcgcg gtcgcatcgc cgtcaccgtt catgcagtgc agcgtgaacg tcgtatcgct
     3721 cgagagtgtg agcatcgtct ggctgccgct cgtgacatcg ccgacgccat cgatctgaca
     3781 caccgggacc tgatcgttgc tgtcgagcac gaatgtgaag gtgaacgacg tcggcacatc
     3841 ggcgatgacg accgacggaa acgtcgtgaa cgacaggatc cgcggcgccg tctcgctgcc
     3901 accgctacat gccgcgaaca cgacgaacag cacagcccat cgcagcttcc ccatgctcct
     3961 cgatgatgcc cgagcgacta cgcgccgaac agcttcttga tgcgcaacag caagctcttc
     4021 agtggggatg tgggcgctgg tgcgggcttc gtatcgccgc taccggcgcc ggtcggatcg
     4081 ccgatgagct tctgattctc gacgtgttga tgcagctgct ggagcagtgg cgccacgagc
     4141 gacgcgaagc gctccgcgag cgcagcatcg atgtcggtaa agcccggccg ccgcgggcgc
     4201 cgtgccgcaa cgatgatcgc ttggacgtgc tggtcgctgc ggagcactgg ctgcatgagc
     4261 agctggctgt tcgaatctcc gtcgggatca tcgatcggtc caagccaccg cggatcggaa
     4321 gccgcgcgct cggccagcgc cgcccgaccg gtgtgggcca cccagcccgc gaggccggcg
     4381 atcgagcggc ggttgtcacc catcgagagc ttgcggcccg gtgagctgag cgcgccgctc
     4441 gcgtcgtcgt acagcaagaa gtacgcgcga tcagcgtcga gaagctcgcg cagcgtcgag
     4501 accgcggtgg tctccgcgct gacgaaatcg accgcccctg caaacgcgcg cgagccagtg
     4561 aacgcgatct gcgcgcgacg cgcttccgtc ggattgttcg ccttcccaag cgactcgttg
     4621 ctcgacagcg ccttgcccgt cgcgaccgcc gtcagcaaat ccgcgaggtg gtcgttgctc
     4681 atggtcgtgc gcgcgacata gacgacgcgt ggatcgatat cgggcgacca cggtaggccg
     4741 agcgtcacga gccgcgcgtc ggccggcgcc gactcggttg tcgacgcaag cgcgatctcg
     4801 aggccgcatg tcttcgcgat ccgccgtgcc cggtcatgct cctcgtcgcc tgcaacgatc
     4861 gccacgcgat gcgccatcgc gatggtcgcc gacggagcag gcggcgatga aggcggatgg
     4921 cgcggcgcag gcagcggcag cttctcgacg agcggtgccg agggccgcgg aatcggccct
     4981 gtcgaaggcg ctcctgcgga ctgtccggaa cccggacgat gcccggtgac gggattgggg
     5041 ccggtcggcg ggcgctttcc ggtgatcggg ttcggtccgg tcggcgggcg atgacccgcg
     5101 atgggattcg gtccggttcc cggacgatgt gccgcgatcg ggttcggccc ggtcggcgga
     5161 cgcgacgagc ccggtgccat ttcgggtcgt gtctcgtccg tggggttacg cggccgcgcg
     5221 gaggagggcg agccaccgcc gggcgtcgac gcgcgcgatg acccgcccgg aggtgtcggg
     5281 ctgcgcggcg cgccaccgct cggcatctca ggacgtgtct cgtccgtcgg atttcgcggt
     5341 cgcgacgccg tcggcgacgc ggtcgcgggc gtcagcgcgt tggggcgaga ctcacccgtc
     5401 ggcgcgcggg tgcgacccgt cgtcgaggtc gacggtggct ggctcgtcgt gcgcaggcgc
     5461 ttggagtcgc tcgcgtcggc gggctcgtcg acgatggggg cgcgtggtgc cgtgggggcg
     5521 tcgatgctcg acgacgagct gcggccgccg tcgatgccgg cgctcgagtc gcgcgtgggc
     5581 ggcgacgtgg gcggtcgctt catgcccggt gccgacggtg ggcgagggcg gggcgcggtg
     5641 agcggcgacg gccccgtcgg cggcttcgtg cgcggcggcg cgatcagcgc ggcagtttgt
     5701 gcgttcttga gcgcgccggg aatggtcgtc cgtggcttgc gtccgtcggc gaccggcggc
     5761 gcaggcgcga cgaggtcgag cgtcggcgcg acgagcgccg cgagactcgc gagcgcggct
     5821 gcgtggctcg gcttgaatgg gtcgtggcgc caacggcgca ccgcgacgag caccgcgtgc
     5881 gtccggcggt ctccgccgat gatgggctgg acgatcaggc gcgtctgcgc tttgccctcc
     5941 ggatcgtcga gctcttgcag atagcgcggg tcgtcgccag ccggctcggc gtgcagcgct
     6001 tcgccggtgt acgccgccca gccgacgaag ccggcgatcg cgcgccgtga atctccgagc
     6061 gtaacgcgcg cgccctccga ccacagccca ccactcaatg gatcgaagta caggcagtgc
     6121 gcgcgatccg cgccgacgac ggccgtgatc gtgtcgacca tgatcgactc gatcgcggcg
     6181 cggtcgtcga gcttcgcgat gcgttgggct gcgtcgagca cgcgcgggtc gacctcggcg
     6241 gacgcctcgg gaagcgcggc gacgacgcgg ccgctcacga gcgaccgaat cagatcgacg
     6301 agctggcgat ccgtcaccga cgggcgcacg acgtgtgcga cgcgctccgg tgcgggcggc
     6361 gacggctcgc cgatcacgat gacgtacagc gcctcgtgcg cgatcgcgag gctcgacatc
     6421 acgggtacga gcagcgaatg cgtgtggcag aggcgacgaa cgcgatcggc ctccgagcct
     6481 gtcgcgacga cgacgacgcc acccgatgca gactcgctac gccttggcgc gtcgtcggtc
     6541 ggcgcgttgc ccgacacgcg atcgagcaag atgacgccgt cctcggtcgg gtggcggcct
     6601 ttcgcgatcg cgggcgtgcg accttgcgca ggcatgccgc gcgcggtgct gctcggatcg
     6661 atctcgacgt cgccggctgc ggcgtagagg gccgaggcga tgtcaggcga gctcgtcgac
     6721 gcgaggtcgg gcgacgctgt catgggtcca cgcggcgcga ctggaatccc gcggttcgtg
     6781 ctcgacggat ccggctgcac cggaatcccg cgatcggtaa cggtccgact cacgtgcatg
     6841 tccagctgcg ccgtcgacac gccgtgcggt ggcgtcgtcg gattgccttg tgccggcgtg
     6901 cgggtcctgt cttgaaggcc ttggttcgtg gtccggcgac gcacgttcga cgcagcgtcg
     6961 tcgatgagcc gctgcttcat gcgttctttg cgaagcgcgg cgacgtggtc atcattcgcg
     7021 cgcatggtcg gctgatcgtc gccgtccatt tgcgcgctgt cgtcgtgttg cgtggcgtcg
     7081 tcccacggac cgtcgtccgg cggcgtcacg gctatttccg cttcttcttg ctcgcggggg
     7141 acttcttggc ggcaggtttc ttcttcttcg cggcaggctt ctttgcagcc ggcttcttct
     7201 tcgccgcggt cttcttcttg gctgcgggct tcttcctcgc tgcgggtttg gccgcgatct
     7261 taggaatcgc ctttgcctga tccaacagct cgcgaagcgt cgcgtactcc gcccagtccg
     7321 gatcctcgtc gtcgtagaag ccgagggccg catcgaaggg cgagaccttc tcgatcagct
     7381 gctgcggatc accgccgccg tacatataga ggaagaacga tccgtcttcc gtcttcgtcc
     7441 cgacgatctc ccacccgagc gtctgacacg cggggcggag ccattgctct tcgagatcgg
     7501 cgacgcgccg gtacgcggcc gcattcggga gccgtgacat ctcgtgggtc gagtagggca
     7561 gctcgaccac gaagatgcgc gtcagatcct tcttcggatc gagctctcgg atgcggaaca
     7621 tggtgggcgt tccgtcgtcg cgcttggctt cgacgatgtg ccacagctcg ccatccatgc
     7681 tcatgagggg cctaacctac cgcaacttcg tccctcactc cgaaatagtc ctggacgata
     7741 gtggttcgcg attggccacc gcatgcctta aaatattcgc catgcgctgg gtggtagcag
     7801 ctgcgctggc gctcgtggcc tgtcgtggat caacgagcga tgggcagcaa cctagccgcg
     7861 atttgccgcc gacaccgaac gcggccgccg ccacgacgcc ggcgaagaca catgtcgagg
     7921 gcagctcgac gatgcgcccg catcacgagg agcccgtcat cgaatgctcc gtccacgtcc
     7981 ccgacattgc cgacgacgag acccgcgtcc aagcgctgct cgaccaggcg aaccgcgaga
     8041 tcgaacgcgg cagctacggt gcggcctgga cctgcgccga ccgcgccgcg gacctggctc
     8101 cgacatccgt cgaggcccac catctccgtg gtgcgtcgct ggccgcgctc aaccgcgata
     8161 gcgaagccca gctcgcgtac tcgctcgcgc tcgcgctcga tcccgatgat cccgaaacgc
     8221 tgcgcgccgt cgccgacttc tacatcaatg gcaaaggcga ccgcggccgc gatgccctcc
     8281 gcctcggtct cgagctggcg caacgcggca gccgccgcgc gctcgctcgt cgtcgtcgca
     8341 atgcgccgct tgccgccgac ctcgccgtcc tcgaggcaca ggcgctgaac gacctcggtc
     8401 gcagcgatga agcgctcgag cgcgtcgatg ccgccgtgcg aatcgcacca aaccgtggcg
     8461 atgccatgca cgagcgtggc gtggccctgt tcgatctgtc gcgcttcgcc gacgcaaagg
     8521 tcgcgttttc gaaggctctc cagagccagc cgcaggacgc gtacacgcat cagatgctcg
     8581 gcctcacgct cgagcagctc ggcgaattcg tcgctgccga caccgagcag aagaaggccc
     8641 tcgagctcgc gccgcacgag ctgaccccgc cggtcctcat cccggtcgac gagttccaaa
     8701 aggaaatcga ttccgtcgtc gcgacgctgc cggccgaccg ccgcgcccac gtcgacaaga
     8761 tccgggtcga aatcgccgac ctcccggacc ccgacgacct caaggccgtc cagccgccat
     8821 tcccgccgac gatcctcggc ctctatcgcg gtcccgtcgg tcgcgctccg acggcctccg
     8881 acgaggcacc gtcgatcgtc ctctaccgca agaatctcgc gcgcgccgtg aagactcgcg
     8941 tcgagctgtc cgagcaaatc cgcgacacgc tgctgcacga gatcgggcac ctcgagggtc
     9001 tcgatgaaga cgatctgcgc cgccgcggca tggaatagct agcgccctgt cgatcgaacc
     9061 gtaggctggg gtacgatgcc gaacggatgt cgtcggacgg cacgggattg gcagcgagcg
     9121 taccggactc ggtacagacg ctctcgtccg cgctccgccg cgagcagaag aaggtcgcgc
     9181 tcgtccaaga ggtgagcaag gcgctgtcga ccgtcggcga tatcgacgcg ctgcttcgcc
     9241 tgatcatgtc caaggttacc gagctgatgg aggccgaccg cggcacgctg ttcctcgtca
     9301 ccgaagacgg ccgccagctc tggtcgaaag tcagccaagg ctccgaggtc gtcgagatcc
     9361 gcctcgacat cggtgaaggc atcgccggat gggtcgcgca aacgcgcgag atcgtcaaca
     9421 tccccgacgc gtacgccgac caacgcttcc agcccgccgt cgatctcaag tcgggctacc
     9481 gcacgcggtc gatcctgtcg gtgccgatgc tcggtgcgct cggtggcctc gtcggcgtga
     9541 tccagctgct caacaaatcc gacggcccgt tcacgctcgc cgacgaagag ctcgccagcg
     9601 cgctgtcctc gcaagccgcg atcgcgatcg agaacgcgcg cctctaccac tcgctcgtca
     9661 cgcagaacca agagctgtca cgcgcccgcc gcgacctcga gcgtcgccaa cgcgagctca
     9721 acgcgctcta cgaagtcgaa aaagagctct ccgcggcact cgacctcgac gaccttctgt
     9781 cgcgcatcct cgcgcaagcg atcacggtgc taggcggcgg cgccggctcg atcgcgctga
     9841 ccgaagccga cggctcgctt cgtttccgca ccgtgcaagg cccagccgcc ccgcgcctca
     9901 tcgagcgcac gctgccgcac ggcacgggcc tcctcggctg gtcgatcgcc cacaaaacgc
     9961 cgatcatcgt cgacgatcca gcgaacgatc accgccacgc ccacgaggtc gccaaagaag
    10021 cgggcgttcg cccgcagcac atcatcatcg cgccgctcgt cgacggagaa gacgtgatcg
    10081 gcggcatcga gatcatcgat cagcgccgta cggttcgcga cggcgagaat gcgtggggcg
    10141 aagaggacct caagctcctc gtcctgatcg ccgcacaagc cgcctccgcc atcgggctcg
    10201 cgcgccgccg ctccgagcaa tccaatcgcg accgcctcgc gtcgatcggt cggatgctcg
    10261 cgggcctgct ccacgatctc aagaccccga tgacgatcat ctcgggctac gcgcagctga
    10321 tggccgcgtg cgacgaaccg ccccaacgcg agacgtacgt cgagcagatc cagcggcaat
    10381 tcgatctcat ggcaggcatg actcgcgagg tgctcgcatt cgcgcgcggt gacgccgacc
    10441 tcgtcgtgcg caaggtctac gtcaaccgct acaccgagga gcttgcaacg cagctcggtg
    10501 ccgcaatcgc gggccgctcg atcgagttca tggtcgaagc gcgctacgac ggcatcgcgt
    10561 acttcgacga gcaaaaattc ctgcgcgtgt tccacaacct cacgcgtaac gcggccgagg
    10621 cgatgccgga cggcggcaag ctacgcgtca tcgtcgaaag gtcggtgacc agctcgtgtg
    10681 gaccgtcaag gataccggcc cgggcatccc gcccgagctg cgcggccgcc tcttcgagct
    10741 attcgcgacg ggcaaaaagg gcggcaccgg cctcggcctc gcgatcgtca agaagatcgt
    10801 cgacgatcac cacggcgtgg tcgagtgcga atcgggtagc accggcacca cgttcatcat
    10861 caagctgccg atcgcccgcc cgaccgacgg cgattagtgc gcgccgaata ggtcgcgaat
    10921 cgcggcgatg agctcgggcg ttgcgccgtc gacgccgatg cgcagcgtga tcgtgtcgcc
    10981 gccgccccat ggccaccaga gggcgaacag cttcggggcg aacatctcgt cacccgcgag
    11041 caggcgctgg ccggagcgga ggccgcccgt gcgctcggcg atcgcttgca tgtcgggtgg
    11101 cgcgttctcg agagacttgg tcgtccagcc gcgcgggaaa gcgttcatcg cgctcttgcg
    11161 tgcgtcgggc tccatctctt tgctgaacga cgcggcgacg gtctggaagc ggctgtccca
    11221 cgtccatggc gggtttggcc agctgtcgcg caggagcgtc aggcgctcga gaagcagttg
    11281 cgagctatcc atcagcgccc ttccgatctc atggcgccac gctatcacga ggtcgtgcca
    11341 aactcggcgc gtgctcgagc tgcgcggcat tcgcaaggcc tacggcgcgc gacgcgcggt
    11401 cgacgatgtc tcgctcaccg tcgaggcggg gcaggcggtg ttgctcgtcg ggcgcaatgg
    11461 ggcgggcaag acgacgctgc tgcgcatcgc gacaggcttt ttggacgcgg atgcgggagc
    11521 gtgacgatca gcggcgtcga tctggcgaag gatcgcgcgg gcgcgcaggc aaagctcggc
    11581 tacttgccgg agcacgcgcc ggcaccgacg gacctcagcg tgcgtgcgca cctgacgacg
    11641 cgcgcgaggc tcaagggcgt gcgcaagggc gcgggcgacg aggtcgagcg cgtgatgaag
    11701 gcggcggcag tggatagcga ggcggagcgg cgcatcggga atctgtcgaa gggctatcgg
    11761 cagcgcgtcg ggctcgcgga tgcgttgctg ggatcgccga agctcctcgt gctcgacgag
    11821 ccgacgagcg gcatggatcc gatccagacg aaggagctgc gctcgctgtt gatcgacatt
    11881 gcgaaggagc gcgcggtcat cgtctcgagc catgcggtcg ctgacttcga ggggctcgcg
    11941 gcgaagctcg cggtgatggc ggcgggcaag ctcgtcgcgt tcgagacgcc ggagaagatc
    12001 atcgagcaag cgggcgcagc gaagctcgac gaggcggtcg tgacgttgct cggcgagggc
    12061 gatgcgaaga agaaggcggc ggcatcgtga gcgcggcgcg ctcggcgtat tgggtctggc
    12121 gccgcgagct gcaagtcacg ttgcgcgcgc cgatcgtcta catcatcggc gggctgtttc
    12181 tgatcgtgca gggcgtcgcg tttgcgggcc tcgttaccgt gctctccgat ccgaagaagc
    12241 cggcgcctct cggtgcgctg ctcgaggggc agctggcggg cacgctgctg acgtgggtct
    12301 tgcagctcgt cgtgctgacg ctgctcggca tgcgcacgat cgccgacgac aagaagaccg
    12361 gcgcgtggga gctgctcttg acggcgaggg tgggcgaggg tgccgccgtc gtcggcaagt
    12421 ggctcggcgc agtgacgatc tacgcgctgc tgtggctgcc gacgctcgcg tacctcggca
    12481 tcgtcgcgat ctttcgcgtc gactcaggcg gctgggatgc tccgagcatc atcaccggat
    12541 acctcggcgc gatcatgctc ggcgctgcgc tgctcgcgtg gacggtcgcc gcgagcgcat
    12601 cgacgtcgaa catgctcgtc gcaggcgcgc tcggcttcgg gatgctgatc gtgttctttc
    12661 tcatcggcga gctgcccgcg gcgtttccgg acctcgcgat cgatcatccg acgctggccc
    12721 gcgcgctgga ggcgttgtcg ctgcgcggcg agctcactac gtttgcgcga ggcgagctct
    12781 cgctcgtcgg cgtgctgtat atcgttgggc tggcgctagt cggcctctcg ctcgccatcg
    12841 cgctggcgtg cggcggtcgc cggcagcgac gtgaggtccg cacccgtgtg ctcgcgactg
    12901 cgctcatcgc gatcatcgtt gtcctcggcg gcgtggtcgc gctgcgccgt ccggtgagcc
    12961 tcgatgtcag cgctgcgcgc cgcaactcgc tcgatccgga gacgcggctc gtgctcgccg
    13021 aattgccggg ccgcgcgaag ctcacgatcg tgcgcccgac gctgagcgcg ctcgagccga
    13081 tctacgatga ggtcgagcgc gtcgccgcgc gcatggcagc gtcgggcgct gtcgatgttc
    13141 gcagctacga tccggcgacg ctaccgggcg gcatcgcggc ggcagcgcgc gcagcgggcg
    13201 ttgcgcccgg cgatctcgaa tccaacggtg gcgtcgtcgt cgaggtcggc gcgaggcgtc
    13261 gcgtcgtcga tcgattgcag ctcgtggcga tcggcgtgaa cgcgagcggc actcctgtcg
    13321 tcgagcagct cgagatcgag caggcgatcg ccggcgcgtt tgccgagctc gcgcgcaacc
    13381 agccgctcaa tgtctgcgcg acgacgggcc acggtgagct gccgctcgtg cacgacccga
    13441 aggatgcgga ttggtcggcc gtcgtcgagc ggttgcgcgg tgagggcatg acggtggcgc
    13501 ccgtgcagat cggcgcgggc gtcccggagc attgcaaggt cctcgtcgtc gcgggaccga
    13561 ccacgccgct gtcgtcggcg gaagcgatcg gcgtgcagac gttcctgcaa cgcggcggct
    13621 cacttgttgt cgcagcagcg agccgcccgg tggtcgcatc ggttgcggcg gccgggcaag
    13681 agaccacgct cgcgtcgacg gggctcgaag cggtgctcgg tacggtgaag ctcggcctac
    13741 cgccggcgat cgcggtcgat ccgacgctga ccgtgcgcga gctgcccggc gcgctgctcg
    13801 tgatcgacgg ctatgccgcg catccgatca acgatgggtt tccgcaggcg cgcgcgaccg
    13861 tgtggttcca gccgcgtgtc gtgctcgcga cgggcaaggc gcagccgctc gtgcgcgctt
    13921 cgacggcgag ctggggcgag cgtgatctcg tctcggcgcc gccgcagaag aacgaagacg
    13981 atgtcggtgg cccggccgtg ctcgccgcga tctcggagaa cggccacgtg atcgcgatcg
    14041 gctccgcgga gtcgttctcg acggccgtgc tgaaaggcgg gctctcggcg agcgaccggt
    14101 ggctcgcgca cgcgatccga ttcgcatcgg gcacgccgct gccgaaagtc acgctcggat
    14161 cgcgtggcct cgatcaagtg cggctcgtgc tgacaccggc gcagcggcgc atcgtcacct
    14221 ggctctcgat caccggcatc ccgctcgtgt ggacgttgtt cggcgcgctc gtgatcgtgc
    14281 tgcgcaggag gaagaacacg tgatcgcgac gacgcgcggc ctcatcgttc tcctcgtgct
    14341 cgccgtcgtg ttcggcgtgc tcgtgatcgt cgtcggtccg cgcaagcgcg agtcgctcga
    14401 tcgcgcactg atgccgcgct tcgacgagac gcgtgtgaac gagctcgtct atacctgccc
    14461 cgaaggcgcg gagaccgcga ttcgcgatgg caagagttgg cggctgaaga cgggcgtcgc
    14521 cgatgcagcg accatcgacg cgacgatcgc ggcgctccgc ggtgcgcgct ggcatcgcag
    14581 cgccgagatg acgcgtgccg gcaagacctc gtgcacgctc accgtcggtg acgcgatctt
    14641 cgcaatcggc gagaagctcg aaggaaccga gcaaacgtgg atcttgcgag gaaggcgcgc
    14701 gtacctcgtc gatggctggg tcgcaaatgc gctcgcgccg ggccgcctcg cattgcacgt
    14761 gcggcgtccg ctcgcggatg cgaaggtcga tccgcactcc cacggcatct ggatcgatcc
    14821 agcggtcgtc caggacattc gcgccacgct cgacgcgctc gagctcgtct cgctcgacgg
    14881 caaagagggc acgcccggcg cgaaagcgtt cggcgccgaa cagctcggca gctgcgacca
    14941 gaatcgcgtc tacatccgcg cgccgagcgg cgtgggctgc gtcgaggcag cggcgtggca
    15001 agccatcgtc gatgcggagg caaagctcgc cggcccgccc gaagcgatcg ccgaccgccg
    15061 gcccgtgccg atcacgccgg tgaagctcgt cgtgtctgat cggcatgaca tcacgctcgc
    15121 gggcaagccg cagatcgacg gcgtcgtcga tgcagatgcg gagcgcgtcc gcgagctact
    15181 cacggcgctc gcaacgcccg gcgagctcgt tcccgtcccc gagacggcac cgaccgatgc
    15241 gctgctcgcc gttgccgccg acgggaccca ggtgcagctg cacatgttcg gcaacggcgt
    15301 cctctatcga cgaggcgagc cgatcgcgat caagacgccg agtgaacggc tcatcgcgag
    15361 gccgagcacc gattaccgcg atccaacgcg ctggcgcgag gatccgacga cgatcacgtc
    15421 gatcaagatc gacaacgtca cgttcgagcg cggcgccgtc ctcggcgaat ggacgcgcaa
    15481 gccgcccgcg aagccggggg cgactgtcga caccgcgctc gtcgatgcgc tcgcgcagac
    15541 gctcgccgtg gtccgtgcgc catcgaatgc cggcaacggc aagacgacga acggccgcac
    15601 gctcaaggtg acgttcacgc cgccggcggg cgatccgtcg acacacacgc tcgtcgtcag
    15661 cggcgcgatt cgtgacggct gccgcggtcg cgccgacaac agcgaagtga ttttcccgcc
    15721 cgagctttgc accgcagtag cggcgttgcg tccgtgatcg tgagacgcta aacgtcgatg
    15781 tggagggtcg ccggattggt gctgctcgcg gcgtgcggcc ggggcttctt caatcctgat
    15841 ctgtcgccgg accctgacgc ggccacgccg gtaaccgcgc cgtcgtatgt gcagtcgaac
    15901 tacgcgacgt cttcgatggg acaggtcacc gcaatcgatg cagcgttccc actgtcgcag
    15961 accgcgggta acaccatcgt cgtggtcgtg atgtgggact cgacgaacgc ggcgctgctc
    16021 acgctgttcg atcgtgcggg caacacgtat cgatccgccg cagggcccac gctcacgaac
    16081 ggaaactgga cccagacgtc gttcatcgcg acggatatcg cagggggcgc cgcgggaacg
    16141 aacgtcgtgg aagccgattt gaacgcgagc tctcccatgc gcatcgccgt cctcgagtac
    16201 gcgaacgtca gcaccgcaca accgctggat ggcaacgcgc aggcgaaggg tgactcggga
    16261 atgctgccgg caatctcgat cacgacggcg aatccaaacg agctcctcgt cgtcgccgcg
    16321 agcaccgatt acatgttcac cgacgtcttc ccgacaccag ccacgcgagt atccggcacg
    16381 aatttcttcg tccaggacaa agcggcgcca gtgccggcaa cccatgtctt tgcgactccg
    16441 aacgggacgt cgaacggcaa ctgggtcgcg caggtgctcg cgctgcaggg ccgatagcgg
    16501 ctggcaggcg aagtcgccgc gtctggcgag tcctctcgtc tgggcgcgaa atctccggtg
    16561 acagccccag tgtcaccgca cacgcgttgt cgacgacttg cgcgcgactt cgtaacccgc
    16621 ggtctacgcg ctgacacgtg gaacatgcgc tgcactcctt cgcgacgtcc ccaacaggag
    16681 tcattccatg agcgatttcg agacactcac cgacgaccag ctttccgaat gcagcggtgg
    16741 ttacggcgcg cagctgaatt ctgcgatgca tcgcgcccgc gagctcggcc ttcgcatcac
    16801 gtcgacgacg ggcggccgcc acgcgccgca ctcgtaccac tactccggcc gcgcggccga
    16861 tgttgcgggg cccgcgtcgg cgatgcgcca gttctatcgc gagatgtcgc gcacgcaccc
    16921 gacggagctg ttctacgatc cgatgggcgg catcaagcac ggccgccaga tcggcccgat
    16981 cggcggccac cgtacgcacg tgcacgtcgc gtactgagac gcacgtcgcg tactgagacg
    17041 cgcttacttc ttcttgacga ggtcttcagg cgagggcagg gcacctggcg gcggcgcgcc
    17101 tgcacccgtg gcgcccggca tctcgaccgg cgcgggcgac tcgtacgcct gcgtgacgtt
    17161 cttcatcgac ttgtagacga tgaggccgtc ggagttgcgc tcgctgtgcg gcgacgacgt
    17221 cttgaaggtg ccggtgatga cgacttcgtc gccgaccgcg tacggcggac agatgctctt
    17281 gctcttgtcc ttctcgtcgg gctcgcagcg atccggctcg acgcgatcct tcttcttgat
    17341 gcgcttcact tcgagcgcgt tgtacgggcg cggcacgtcg acgacccaga ggctcttctc
    17401 gacgggcgtc tgcgccgtgt cgccgatgta gaacttcggg cgctcgcaga gcgtcggatc
    17461 ttcgtcgatg cgcgcttgca cgtccttgag gtcttcttcg cctttacgga tggccgtctt
    17521 gcagtcgtac gcccacgtca cgaagccgtg aacggtgagg tccgtgtcga ggagcttctt
    17581 gcctttgacg cgaagctctt tgacggtgtg cgagccatct gccggcgccg gcggcaggtc
    17641 gaatgccgga acggcgggca ggtcgagctt tacgtcggtc ttcttgacct ccgcctcggg
    17701 gcccttgagc ttgtccttgt cacaagctgc gccagcgagt agtacgactg gcagcgtgaa
    17761 cgcagtggcg aagaccccca gacgatgcga catgcatgcg gtcttaacat ggtgaccaag
    17821 gggtctcaag tccgcgtggt ccttgccatc gcggcgacga tctgcgcatg cgagtccccg
    17881 ccccgggtga aaccgtggcg acatgcccca gatccgacgg cagaggccgc gaatgcaccc
    17941 ggctcgccgg tcctcgcgca ggccgcaccg tcggaggcgc aggtcatcgc gaaccgcagc
    18001 cacacgctgc gaatccacat ggatgcggac cccggccgcc tgacgcccat cgtcgagccg
    18061 tcgatctggg ccaagcggat cacggtcgga acgatcttcg agccgctcct tcgctacgag
    18121 cccgcagacg ccaacggccc agcccgcttc accggtcgcc tcgcgcgaag ctggcagctc
    18181 atgccggtgc cgggcggcat cgtcattcgc atcgagctgc agcccggggt cacgtttcac
    18241 gacggcacgc cgctcacgag ctccgacgtc cagtggacgc tcgacaccat ccgcgatggc
    18301 cgccgtcacg atctcgagca cctgaagccc atgctcgacg acgtgttccg catcgagctc
    18361 ctcacgccgc tcgagctgca gctcgtcctg ctgcgcccga acgcgtgggt gctgcgcgcg
    18421 ctcgccgaga tcccgatcct gccgatgcac gtctacgacg ggagcctgct cgccggtggt
    18481 gcgctcgtcg gaacgggccc atggaagatg ggctcgcaca aaggcggcgt ggtgcacctc
    18541 gtcaccaacg acaagtactg ggcgggcaag cccgcgatca gcgacgtcga gttcgtctac
    18601 gagcccgacg ctgcggctgc actcaccgac gcgaagcgcg gcgagctcga catcgtgccg
    18661 gcgctgatcc ccgcgcactg gcccgagcaa gccagcgcgc ctggcctcca agcatcgttc
    18721 agcgcgctcc aactcgcgcc gccgcgcctg cggatgttcc gcttcaacgc gtcgcgccca
    18781 tcggtcgccg acccgcgcgt ccgccacgca ctcgccctct tgatcgatcg ccgcgcgatc
    18841 gcaaagcgcg tgttcgacgg cctcgcgcgt ccagccaact ggccgatctg gccgggcggt
    18901 cccgtcgacg gcgccgaggt cgcggccccc gacttcgatc cggccgccgc cgccaagctc
    18961 ctcgacgaag cgggctgggt cgacacggac aaagacggcc tccgcgatca aggccccgac
    19021 aagcagctca agctggtcct catcggtcgc gacaaacccg cgcccaaaga ttcctccggc
    19081 ccgccggtga agacggagcg cgattacttc gtcgacgcgg ctcgccgcgc gggcgtgatc
    19141 atcgaggtgc gcagcggcgg cgacagctgg ctcgacaagc acatcgaagg tggcgactgg
    19201 gatttgctcg aagtcgcgtg gtcaggacgc gtcgacatgg acatcacgcc gcttgtcggt
    19261 ggtcgcgacg cgacgcgccc gcagcaaccg cacgtcgatc gcgtgctcga cgcgatgacc
    19321 gcggcgtggg atccggcgga gcgtggcaag ctcgcgaagg agctcgcggt cgcgctcgcc
    19381 gagagctggc cggtggccgg catcaccgcc gatgctccgc agggcctcat ccatcatcgt
    19441 gtcgagaacg taaaagtctg ggacggctgg ctcgatctgt cgcaactccg cttcgcgaag
    19501 tagcgtctgc gcgcaccgct cattccctgt cgttcccaga tggaacgcaa ggaagctagg
    19561 ccggacaggg acgcaagcag agagcgcgat taggcgcgcg ccgtgcgtgc acacgcgtga
    19621 gtgcagagcg cgacacatca cgtgtcacgc cgaaggcgcc ccggaccctc cggcttgcgt
    19681 ccctggctcc ccttgctccc ttgcgttcca tccgggtctg cttgtcgctt gcgacgaacg
    19741 agcttgcgac gaacgagctc gcgacgaacg agcgatattc gagctcagat cgagtggagc
    19801 gagatgccgt actcgatctg cgtcgacgtc tcggccgtcg ggaacgacca gtgcgagaca
    19861 gcgtgcacga tgcacttgtg cgcgcccgcg gggacatcgc cgccgagctc gacgtccgtg
    19921 acttgacccg cgtcgttgat cgtcaggtcg aggtcgaccg tacacgcgtc ggggcgttgc
    19981 tttgccggga tgcggctcca gcagagttgg atctcgtcgg cgtgcgagtc gatgtacgtc
    20041 gacacctgcg cgcgcgagag gcccttcggg acgaactgct gaacctcttc ggtcggcttc
    20101 gcgctcgtgc gcttgctgcc gccgccgagg acgtagtcgt tgcccatctt catgacgggc
    20161 gccttctctt tgaccggctt cgcgatcggc tttgccgcga ggacgggcgc aggcagcgag
    20221 acctgaccga agacgccggc cgtgtcggcg ggcagctcgg gcagggcgag catctcggtc
    20281 gccgccgcgg gctgtgcgat cacgtcggcg atcgcgaggg ccgcgccggc accggcttcg
    20341 gccgatgcgt cggcgacgac gacgacggcg ggtttggcaa tctccgcctt cgcaggcgcc
    20401 ttcgcggagg tttgcttctt ttggtttcca gcgaccgcag gcgtggcgag gagcccggcg
    20461 gtgccgacaa ctgcgacgag gatggctttc atgggaaggg tccaagagca cccgccgtgc
    20521 cacgcgcgat cctgaagcgc tgcgcggact ttcatgctga cgtggagtca ggcgtccctt
    20581 gggactctcg tttcccagcg gaagctcgag gagccactcg cagggcggcg aggaggacaa
    20641 ggccggcggg ctgggcggca agaaccgtaa gcgcgaccgt ggttccggcg gcatcggcaa
    20701 gcgcaccgac gagaaacggc agtgcgaggc caagcggcgt gaagaggtgg ctcgcggcga
    20761 gcacgcttcc cgactggttg ggccggcacg cgtacgcctg cgcggcggcg agagggtaga
    20821 gcggcgcggc gaagatcccg atcgggatcg cgaggagggt tgcggcgagc ggcgagtcga
    20881 cggcgagcca cgggatatac gcgacgacgc agccgatgct cgacacgacg agcaactcgc
    20941 gctcgctgcg gcggagcagg agtcgatcga gcaggagcag gccgacgatg ccgccgccga
    21001 cgaacgcgcc gacgaccgcg ctctgccaga acggtccggc gtgcagaccg acgcgcacgt
    21061 ggatcgacgc gaacacgacg aagatctcgt cgagcagatc gcagagcgca gtgccgaaca
    21121 gccatgcgag cagcacgcga tcggtgagcg catcacgcag cgtcgcgagc aggccggcac
    21181 gcggctcgtc ggaatgctcg gcatcgtgga gattcgcggg cgacgcaggc gcaacatcga
    21241 gcttcgagca cgcgagcagc agcgcccagc cggcgagcaa cccgccgacg atcgcgaagc
    21301 tcgcacgcca gctcgctccg gcaaatgcga tcgccgcgag cagcgccggt gccacgagat
    21361 cgccgaacgt cgaccacagc gaccagcgcg agagcgtccg cgcgcgattg tcgggatcgc
    21421 gatcgacaag cgtcgcctgg ccgagcgaga tcgcggtccc gcccgcgaag tacatgacgc
    21481 cgagggcagt cgcgagcgtg atcggtcccg gcgcgagcgc gcacgcgagc atcgccatcg
    21541 ccatcgccgc gatgcccccg cgcacgaacc atcgccgcgg atagcgatcc gcgagcacga
    21601 acagcagcgg ctcgacgacg agcgcgatcg cgcccggcac gacgaacacg acgagcgcga
    21661 gcacggcatg cgctgcaccg agcgaggtct cgatgtctgg cgcgccgaca atgggcacac
    21721 ccgaggagag ctcgtcgaat gcggcgagtc cgaagaggag cgttgtgacc gagacgagcc
    21781 ggccgagacc agccctagcc gcggatcaaa tgcgttcggt gaagtcgcgc gccataccgc
    21841 aagcataccg gatccgcgcc gcgcggaatt ccctcacgtc tgcctcgcgc ggaatgccag
    21901 gtgttgcctt gccgagccgc gtagtgtgga gcccatgcgc tttatctcga tcgtgggggc
    21961 gatcgggctc tccgcgtgcg tcacgcacgt gagcaggctc gggccattcg taaagaatct
    22021 ctcagtgcaa ccgcaaggga tcgtcgtcga cagctgcgtt gtccagtacg agacgaccac
    22081 cgattacacg ccggcctccg gcggcaatcg caaggaccac gagcttgtcg agggcacctg
    22141 ctatcgggat gtcgtgccta cgggaggcgt tcgatgagag cgcttgtcgt atgtgcagcg
    22201 ctgctcgcag gctgcgtgaa ccacagccag gtcggtccgt acgtgaagag cgtgtcgcgc
    22261 gcggacaact cgctgatggt gatccgctgc atgatcgagc tcgtcggcga agacctccga
    22321 gagggcacgt gcgagcgcct ttatgtgccc cttggctcga tgccgctcgt cccgccgccg
    22381 ccaatgcagc cgccacctca gatgaagtag cgctcgtaaa tcgcgtagcc gagcgcggcg
    22441 gcgcacagga gatagaggag gacgacgagg ccgatgccac cttgcttgac cggcgctgcc
    22501 ggcagcgatc cggtgcggcg ttgggcggcg gctggaaccg cgcgtgcggg cgcgggcgta
    22561 tgcgtcgttg cgccttccgc gatgggacct gcggaaatga tgatcttggg cgcctcatcg
    22621 acgcgccgat tgtcgtcgag gttctccgac tttcggcctt ctccagcggc gatggcgcct
    22681 tcctcgctgg ttcgcagggt tcccacgcct gaatggtata ctggctttcg cgcatggcgc
    22741 atctgctctt gatcgacgac gacgcgcgcg gcgaagcggt cgtcaaagcg ctgaccgatc
    22801 agggccacga ggtccgatgg gcgcgctcga tggcagacgg cctgcggcaa tttcgcgacg
    22861 accgcgcgga cgtcgtgctc gtcgatacgg atcttcccga tggctcgggg cacgacgtgc
    22921 tgcggaccgt gcgtatcgaa gacccgacga ccatggtgat cgtcacgagc gcgaagggca
    22981 cgatcgaaga agcggtcgac gcgatgaagc agggggcgtc tgacttcgtc cgcaagccgc
    23041 tcgacctgcc ggacctcgcg ctgcgcgtat cgaagtcctt atcgagccgc cgcctcgtca
    23101 cgcagctcga gtacctgaag gcgcagcagg cacgcggctc gatgatctcc ggcctcgtcg
    23161 gcgagtgccc cgagatgcga gacgtgttcg gcacggtgat ccggctcggg cagaagacag
    23221 gccgccgcgc gggaccgacc gtgctcatcg ctggcgagac cggtacgggc aagggcgtga
    23281 tggcgcgcgc gctccactac aacagctcgc gtcgcgatgg cccgttcgtc gaagtgaact
    23341 gcgcctcgat tcccgagaac ttgatggaag cggaagtctt cggcgccgag cgtggtgcgt
    23401 ataccggcgc ggtcgcggcg cacgtcggct acgtcgagac ggccgaaggc ggcacgctgt
    23461 tcctcgacga gatcgggtgt ctctcgatgc cgctgcaagc aaagctgctg acggtgctcg
    23521 agtcgcgcac gtatcgccgc attggctcga gcgtcgagcg gcgcgcggac atccagaccg
    23581 tcgtcgcgac gaacatggac ctgcacgcgg cggtggaccg cggcgcgttc cgcgaggacc
    23641 tgctgcatcg gctcgagatc atcgtgctga agatgccgcc gcttcgcgat cgcggaccgg
    23701 atcggctgcg actcgccgcg cacttgcgcg atgaaatctg ccgcgactac ggcctcgcgc
    23761 cgcgcccatt cagcgaggac gtgaaggacc tcatcgaggg ctatcactgg ccgggtaacg
    23821 ttcgcgagct gcgcaacgca ctcgagcaga tcctgctgct cgaggatgcg ccgatgatct
    23881 ctgcgcgcca cttcatgctg cgcggccgtc gccagacgcc gacgaagctg ccgcgcctcg
    23941 gcaccgataa cggatccgtc gagctcgacg tgcccgaaga ggggctcgcc ctacaggccg
    24001 tcaccgacgc gctgatcgac cgaacgctgg cgatgtgcga gggcaatgtc tcgcgcgcgg
    24061 cgcgcatgct cggggtcagt cgcgaccagc tgcgttatcg attggcaaaa cgcggtgcgt
    24121 gagattcccc aattggggaa atccacccaa ccattcccca accgtgatcg tttgatgtcg
    24181 cggcgttgac gcggaccgct tcttgcgaac aattttccat ccacgtggaa cagcggaagt
    24241 actgtgtagt ctgcggcaac atctacagga acgagctccg tacatgtcct gaagatggct
    24301 ccatgctcgt catcgaggcc gagcagggcg agcgtctgcg actgggccac gtgctcggaa
    24361 actaccgcct gatccgcaag ctcggcgaag gcggcgtcgg caccgtctac gaaggcgagc
    24421 acatccggct cggccgcaag atggcgctca aggtcctgca ccccgagacg gcgagcgccg
    24481 aagtgatcgt ccgcttcttc aacgaggcac gcgcggtcaa tcagatccgg catcccaaca
    24541 tcgtcgacgt cgaagacttc gtcaccgccg agacaggcga gcactacctg atcatggagc
    24601 tactcgcagg cgaggacctg cgcacgatcg ccacgcgcga aggcgtgctc gatccacgcc
    24661 gcgtcgcggc gatcggcgaa cagatcgcga gcgcactcgg cgcgatccat ggtgcgggca
    24721 tcgttcatcg cgacatcaag cccgacaaca tctacgtgtg tcgcaaaccg gacggcaccg
    24781 agatcgcgaa gctcatcgac ttcggtatcg cgaagtttct cggctccgcc gaaggcatga
    24841 cacgtgaagg catgacgctc ggcacgcccg agtacatggc gcccgagcag atcctgtcga
    24901 gcggaaagcc ggggccggcc gccgacctct attcgctcgg catgctgatg tacgagtgcg
    24961 tctcgggtgc gcccgctttc accgcgacga cgacggcagc gatcttgcgc ggccacatca
    25021 gcgagcccgt cgtgccgccg tcgcttcgtc gaggagagcc cgtgcccccc gtgctcgaag
    25081 cggcgatcct gaagtgtctc gagaaggatc ccaagaatcg gttcgcgagc ggcgacgagc
    25141 ttgcaacggc gctgcgtgcc gagcgcgcgg tcgatctcat cacgccacgc ttcgagccgg
    25201 gcaagcctcg tcgcaagagc cgcgcgctgc agatgctccc cgcgttcgcg atggcggccg
    25261 ctgcagcggt gttgcacttc ttgccgaagc cggagaacca agcgcacgcc gaagtggcag
    25321 cggctccgac gcctgctcct gtcgccgtcg tcgagaaacc ggcggaagct gctccggctc
    25381 ccacgccaac gcctccaccg ccggcggcac ccaaagcgat ggatgtcgcg ctcacgtcga
    25441 gcccggacgg cgcagagctc tacatgggtg aagagccgct cggccccgca ccgcagatcg
    25501 tttcgattcc gatctcgagc accgccgtcg agctcacggc tcggttcgac gacggcgtcg
    25561 aagtcgtgca gacgatcgtt ccggatcgac cgctgccgca gatcgcattc gtaaagcccg
    25621 cggctccgaa accatcgcag gccgtcgccg tgaagaagac cacgcgcgtg acgcgtccga
    25681 caaaggccgc cgcgacgaag gcggcaccga cgacaacgac gggcgcgggc acgatgacgg
    25741 gcgacaagcg cgatggcacg atggacccgt tcaaatgaag agctgtccga gttgccggcg
    25801 catctacgtc gatagcgagc aggtctgccc gctcgacgac ggccggctcg tcgcgtcgaa
    25861 cgcgcctgca cccgccggtg tgggccgcgc gatgggaccg tacttgctcg tcagccagct
    25921 cggcgaaggc ggcatgggca cgatctatat cggccgccac acgggcctcg gtcgctacgt
    25981 cgcgatcaag gtgctgcggc cggagctcgc gagccgcaaa gacaacgtcg cgcgcttctt
    26041 ccaagaggcg cacacgatca accgcctcaa gcatccgaac atcgtcgagt cgatcgacct
    26101 cgtcgaggat gcgatcgatg gcgcgtactg cgtgctcgag ctgttgcgcg gtcctgatct
    26161 caagacgcgt ctcgcacgcg gcgcgctctc gctcgagtcc gcggtgcata tcggcgtgca
    26221 gctcgcagat gcgctgggcg ccgtgcatgc gctcggtgtg gtccatcgcg atctcaagcc
    26281 cgagaacctg atcctcatcg cgcgcgacgg ccgcgacgac accgtgaagc tcatcgactt
    26341 cggcgtcgcg caaatcggcg gcgaaggcac gcaagccgtg atcggcaccg ctgcgtatat
    26401 ggcgcccgag caagcggtga ccggtgcgag cgtcgatcga cgcgcggacg tctactcgct
    26461 cggcgtactg ctgttcgaga tggtcaccgg acgacatccg tttccgtccg cgaccgacag
    26521 cgagtacatc ctgcgccacg ccgaggacaa gccgccgcgt ccgagcaagc tcgcctcgaa
    26581 gtgcccgccc gcgctcgacg acatcattct gcgctgtctc gcgaagaagc cggagcagcg
    26641 ctacgccgac gccgcgcaag tcgcatccgc gttgcggctc gtcaatgcgc gcgcgggccg
    26701 cagcaaagcg ggcgtgatca tcgccgcggc gttcgtgatc ggcggcggtg cggccgcggc
    26761 ctacctcatc gtgccgaggt acctccagaa aaattcggaa gcgaaggccg agacggtcgc
    26821 cgaagccccc gtcgtcgaga acgcccctgt cgagaagccg gtggagaaac cggccgagca
    26881 agtcgcggag caggcgcccg tcgaagctca gccggcagcg ccgacgatcg tcgagatctc
    26941 gatccattcg aagccagcgg gcgccaccgt gtttcgcgct ggcgagaccg tcgcgctcgg
    27001 cgtgaccccg ttcacggtac agctgccccg ctccgataca ccgacccaca tgcggttcga
    27061 gctcggcggt cacaacgcaa agatcatcga agttcctatc gccgaggaca tggagatcaa
    27121 cgtcaacctc gccaaggaag ccacatccga ggccgttcac gctgtacgat tgaagaagtc
    27181 gacgaagtcg tcgaagtcgt cgacgggcga gggaagcccg ccaccggtgg tgccgtcgga
    27241 caagaaggtg cagcgggaag gggtgatcga tccgttcgcc aaataactgc gaggagcctg
    27301 atgcgtctag ccctgattac gttcgcgatc gcactgatgg ggagtacgcc agcgatcgca
    27361 gatgacgtcg tgatcttgcc gccgctcggt tcggcgaagg atgcaaagcc ggtctccacc
    27421 gcgctcgcag gctccaaggt cggaacgctc gcgaccctca aggtcgacgc ggcatgcacc
    27481 gaagatccgc gctgcctcgc gacgaagggc agcgagctgt ccgcaaaccg gatcgttgcg
    27541 atcacggtga cggcctctgg aaagctcgac atggtcgtcg tcgacgtcgg cgccaagctc
    27601 ttgctaggca cgcgctcgat cgcggtcgcg ccgaagaaga tgggcaagga gctcggacct
    27661 gcgatcgcaa gagcgattga agacatgacc gtcgacaaag cgaaggcgct cttcgcggaa
    27721 ggcaacgaga gctacaacct cggcgagttc gagcgcgctc tcgatcgcta caagctcgcg
    27781 tatcgcgtga agccgctgcc ggcattccag ttcaacatcg cgcagtgcca tcgcaagctc
    27841 ggtcagaaca aggaagcgat cgcgatgtac caggcgtacc tcgtcggcgt tccgaacgcg
    27901 ccgaacaagg atgtcgtcga gtcactcatc gctgagtcga agaaagccat cgacgagcag
    27961 gtcgcggcgc agcgcaagct cgagaccgac aagctcgatg tcgagaagaa gaaggcagaa
    28021 gaagcgcgca aggcgaagga agccgaagct gctgcggcgg cagagcgtgc gaaggcagag
    28081 caggcgcaga tcgcagcgga gcgcgagcgc gcgaagggct acaaccgtca cccagcgcgg
    28141 aagttcatgg tcgttacatc ggtcctcggt ctcgcgaccg tcggcgccgg cgcgtacttc
    28201 ggcattcaag cgcgcgacgc gcagaacaac ttcgacaaca accagtgcgg cgacccgacg
    28261 gttcctcgtc tgcagccgct catcgaccag tgcaacgccg acaaggacaa gggcacgcgc
    28321 gatgcgctgc tcagcaacgt cctcatcggc agcggtggcg ccgtcgtgct cgtatccgcg
    28381 ctgatctacg cgctcgatcc gggcaacgtc gaagctccca aagagaaacg cgtcggcctc
    28441 gctgtatcgc cgacgtccgt gcaggtggtg ctcaaatggt aatgcgaatt ctcatcgcat
    28501 gcgtcgtcct actcggcgcg tgcaagttca acgagcaaga cttcgacgat cgcgcgtgta
    28561 gcgcggattc cgagtgccgt cccgacgaag gatgcgcggc caatgtctgc acgcagaagg
    28621 gctgctccgt cgcgacggac tgcggcatca acggcaagtt cgtctgccaa gagaacgcat
    28681 gcgtcgccgc ggcctgcagc tccgatagcg agtgcggcac gggcttcgcg tgcagcgaat
    28741 cgttttgccg cccgtcgttc aacatcgcgg ctgccgagtc gatgagcaac acgtcgatct
    28801 cggtgacgtt cgatgcgccg cccgatccga cgtctgccgc ggtgctcgcg aactactccg
    28861 tctccggcct cagcctcagc gggacgccgg cgctcgtcgg caataccgtc acgctgacga
    28921 cgtcgacgca gaccgcgacg agctacaccg tgacggtctc cggtatcacg cgtgcgctgg
    28981 acaaggcgcc gctgatcggc gtgagcgcga cattcacggg ccgcgctgca ttcaacgtga
    29041 cgaccgcgag cgcgacgacg agtcgcaccg tcatcctcac gttcgacggc atgccgaacg
    29101 tcacggaagc cgccacgctc gcgaactact cgatcgcggg tctgtcgctc agcggcaccc
    29161 cgctcgtcgt cggtaacacc gtcacgctgc tgacatcctc gcaggcggcg acgagctaca
    29221 cggtcgtcgt caacggcgtg catcgcgccg gcgatgcgga gggcctcgcc gatgccgacg
    29281 cggtgttcac gggccgcgac gacttcaatg tcctgtctgc cgcgtcggag agcagtcaca
    29341 cgttcaccgt gacgttcgat gcgccagcgg atgatctgaa ggcaaagacg ctcgcgaact
    29401 acagcgtcgt gagctcgggc aatgtgccgc tcgcgctgtc gggcgttccc cagctgtcgg
    29461 tcgacggcac cacggtgacg atcacgagtg ccgcgcaagc agcaggtacg tacatcgtga
    29521 cggtcaacaa cgtgacgcgc gcagccgaca acgagccgct caacgtcaag accgcgaact
    29581 tcaccggacg cattcccttc aacgtgttca gtgccatgtc gacatcgagc acgaccgtca
    29641 ccgtcaccta cgacgcgacg cccgtgatgg ccgacgcaat cgacatcgcg aactacgaca
    29701 tccccggtct cacggtgaac agcgccacga tctcgggcaa cacagtcacg ctcgcgacgt
    29761 cgccgcagtc tgggacctcg tacaccgtca cggtcagcgg ggttgctcgc aacagcgatg
    29821 gtgagccgct gacggtcgcc gctgcgacgt tcaccggccg tgcgccgttc aacgtggcgg
    29881 gcgcttcgac gatcacgagc cactcgacga cggtcacgtt cgatgcgcca ccaaacgcat
    29941 cgcaggcgac ggtcgccagc aactactcga tccccggcct caccgtcatc accgcggcgt
    30001 atcccggcac gggcaacgtc gtaacgctga cgacgtcggc acagtccgcg acaccgttcc
    30061 aagtcaccgt caacggcatc acgcgcgcga gcgacggcga ggcactgacg cttgcggtcg
    30121 cgagcttcac cgggcgctcg ccgttcaacg tatcgagcgc actgtccgtg acgagcacga
    30181 cggttacgct gacgttcgac gcgccgccga acgcgacgca ggcccagcag ctcgggaact
    30241 acaccgcgaa caacggcctc acgttctcga gcgtgtcgta cacgggcacg ggcaatacgg
    30301 tcacgctgac gacgaacgcg caagccgcga ccatgtatca ggtgtcggtc gccggtatca
    30361 cgcgcgctag cgatggcgag ccgctcacgg tgacgtccgc gaccttcacc ggccgcacac
    30421 cgttcgacgt agcagccgcg gcatcgacgg gtaacacgac gatcaccgtc acgttcagcg
    30481 caccgcccaa cgcgacgcaa gcgcagacga tcggcaacta ccagatcacg accggctcgc
    30541 tgtcgctcag cggaacgcct gtgctgaacg gcaacacggt gacgctgacg acgtcggtgc
    30601 agaccggcgg tgcggcgtac gccgtgacgg tctccaacgt gacgcgcgct agcgacggcg
    30661 aagcgctgac caccgcaacc cgcagcttca cgggcaagag cggcttcaac gtggcgagtg
    30721 cctcgtcggt gagcacgacc tcgttgtcgg tcacgttcga cgcagcgcct gacatgacga
    30781 cggcgacgaa cgtcaacaac tacacggtgc ccggactgac gctgagtggt cccgtcacgc
    30841 tgtcgggtaa caccgtcacg atgacgacca ccgcgcaggc agggcagacg tacacggtca
    30901 ctgtctcgaa cgtcgtccgc gcctccgatg cagtcgcgct cacggtgaac agcacgagct
    30961 ttacgcacac cacgttcaac gttgcgagcg cggctgccgt cacgagtcac tcgatcaccg
    31021 tgacgttcga tgcgctgccg aacacggcgc aggcgacaac tctgacgaac tacgtcgtca
    31081 aagaccagat gaacaatacg ctcgcactga gcggcacgcc gacgctcaat gcgagcacga
    31141 acacggtcac gatctcgacc acgacgcaga acgggacgaa gaactacacg gtgaccgtca
    31201 gcaacgtcac gcgtgcgagc gacggcacga cgctgtcgac gaagacggcg cagttcgccg
    31261 gcatcggctc gttcgacgtc gcgagtgcaa ccgcgccgac cagcgcttcg atctcggtga
    31321 ccttcaatac gccgcccaat gtcacgcaag cgaccacgct cgcgaactac gcagtgtctg
    31381 gcttgacgct caaaggtacg ccggtcctat cgggcaacac ggtgacgatc gcgacatctg
    31441 cacaaatggc gacgacctat accgtgaccg tctcgaacgt cacgcgtgcg agcgacggcg
    31501 agccgctctc gaatacgtcg gccatgttca ccggtaaggc gcccactgca ccgacggtga
    31561 cgaacgtcgt cgtcaccgga acgaatccga acaacaacac cacgttctac aacaccggca
    31621 cagcgaccgt tgtcatcacg ggtacgcagt tcacgagtgt cgtttgtccg gcaggtgtca
    31681 cgctcgacga tacgaacggc gtcgccgcct cggtgaagac gcccgcgcag tcgtgcacgg
    31741 tcgacaacga tacgcagatc accgcagtct tcccggcggg cattcgcacg aacggcagca
    31801 cgggctggaa cgtgctcgta acgaataccg gcggaacgaa cgctgcgagc tccgtcaagc
    31861 tcgtggtcaa ggcggggttg ctcatcagtg aggtgctcgt cgggctcagc agcggcaata
    31921 acacccgcga gttcttcgag atctacaacc cgaccgcgac cgcgatcgac accggcagca
    31981 ttggcctcgt cgttcacttc cgcgcatcca atggcacgga cacgatgccc gcgctgtcat
    32041 tcctgtcgcc cgccaaccaa caccgtacgc tcgcctcgca cgggttcatg ctcgtcagct
    32101 cgtcgacgtc ggtcgcggat acctggtaca gccaccgcga tgcgacgtac gaccctgtaa
    32161 cgaccgagct cgcgggcaac ggcggcatct atctgagcct cagcaccacc gcgcaagcga
    32221 aggtgatcga caagcttggc tggggcacgc aggtcgccgg cggattcgaa ggcaccgcgt
    32281 tcacgaacat cagcaacaac aacagcgcgc agcgcaagcc cgcgggtggc gcgggcaacg
    32341 cgactgacac cgacgtgaac ctcgctgact tcaacacgca aacgccggcg atcaccccga
    32401 aggggaccgc cgacggtgtc gaaccgtagc tgtcgttacc agcgtcggtg accgcggatc
    32461 acgacggtgt tccgtggtcg ccaagtgccc tggacccaga tgtactggtt cccgcggcgg
    32521 tcccaacgtc cgttgtcata gacgtacccg gctcgctcgc gcacgtaata gccgttctgc
    32581 cagcgccagc cgttgtagcg gtcacggacc cagttgccgt ggacccagac gtagccggga
    32641 cggaaataaa cgacttcctc acgtggtgcg ggcggagcat cgtacgacgc gacgacgtag
    32701 ccatgtgtag tgccggtgca gcccgtcgga acggtcactg cggcggcgcc tgcgagaagc
    32761 aggctgatta gcttcgcctt catacgacct cctagagcga cttagtcgca gccgcggagc
    32821 aagaagatga gcagcaggat cggaatggga attccgagga gccaagcaag agcataacca
    32881 gctgcgcctt tttcgttgtt ggggcgtttg ataagagcgt tcatgacctc gtgaagttgc
    32941 atcgcgcgtg cccgcgtcga taacgtgctc gagtgacacg cgttgccgtg tttggcgcag
    33001 gtgcgatcgg gtgttgggtc ggcggccggc tgagtgcggg cggttgcgac gtcacgctca
    33061 tcggaagatc acgtgtgctc gacgagctgc gcgctgggct cgtgacgagc gagctgcacg
    33121 gtggcacgcg caccgcgaag ccagcgctct cgacagattc ctcagtcatc ggaaatgccg
    33181 atctcgtcct cgtcaccgtg aagtcagcgg cgacaccaga tgcggcacgc gagatcggtc
    33241 cgaagctggg ccccaacacg atcgtcatca gcttgcagaa cggcgtgcgc aacgttccag
    33301 tgctgcgcga cgcgttacca ggccgcaccg tgatcgccgg catggtgccg ttcaacgtga
    33361 tccgtcagcg ggcggggcag taccaccgcg cgtcgggcgg caaggttatg ttcgacgcga
    33421 gcgaccgagc cgcggcattt tccgaggcgt gccttcgcgc cgacctgccg ttcgagcttc
    33481 gcgatgacat gccgggcgtt cagtgggcca agctcgtcat gaacctcaac aacgcgatca
    33541 atgcattgtc ggggctgccg ctcgcgaccg agctgtcgac gcgcgcgttc cgccgctgcc
    33601 tcgccctcgc gcaacgcgaa gcgatcggga tgatccgcgc cgccggtgag ccactcgctc
    33661 gggtgacgct cgtaccgacg cgctggatgc cgggcttgct cacgctgccc gatgcgctgt
    33721 tcaagcggct cgctggtcgc gtcgtcgcga tcgatccgac agcgcgctcg tcgatgtggg
    33781 acgacctcga agcaaaacga ccgaccgaga tcgattacat caatggtgag atcgttcggc
    33841 tcgcggagaa gctcggacgg accgcgccga tgaacggcaa gctcgtgcag ctgattcgcg
    33901 cggcagagca gggcggcaag cgcgacttca gcggcgacga gctgttccgg gctatgcagc
    33961 ccacagcatg acgatgaacg ccgacaccat cgagccgacg agcaggccgt cgaggaaccg
    34021 gccatagctc tgcagcctcg cgcgaaacgg cgccttgcct tcgtgctcga ccaccggcag
    34081 ctccgctcgc ttcagctggc ggaccaggac gtcgaatcga tcccagtcgc gcgcgccgag
    34141 gaaccaccac gagccgcgct ttgcctcgag cgcgacgcca tcgcgataga cgtggcacgt
    34201 cgtgatgcgc gcggcttctt gcgtgaacga cagcgaggtc cagccccacc gcacgccatc
    34261 gcgcgtgacc gcaaccttcg cgcgacggac tgcgaagccg agcgcagaaa acgcgaagaa
    34321 cgcgatcggc agcacgaact gcgtgctcgc gtgccatcgc caccagtgct tcgcgacgaa
    34381 cacccacgtc aacgcgccgc agaagatcac gatcagcaac gaagccgtgg ccagccggcg
    34441 cgcagtcttc ggggcttcga acgagccgag cggcgtgccg acgagaccga tgcccggcgg
    34501 aaggtcgacg actgccatca gctgcccgtg agccccggcg cttccaccgc gagccgcaac
    34561 ctctggagca gcacggcgcg ttctttctcg tcggtgattc gcttgccgcc gcgagtcacg
    34621 tagaacacgt cggcaacctt ctcgccttcg gtcgagacct ttgcgagcga gatatcgaaa
    34681 ccgaggtcgg cgagcgtctg cgtgatcgcg tagaggacgc cgacgcgatc gagcgtgaag
    34741 acttcgacga tcgtggccgc ggtcgaatca tcgtgcagct tgatctcggt cgtcacgccg
    34801 ggcgtcacgc gtttcggcag gccactcttg ggacggcgac gggcgatgag tgctgcggcg
    34861 gcaccgggat cgggaacacc cgcgaggagc tcgcggagat ccgtgagcag gcgcggccac
    34921 cgcggatcgt cgtcggtgat cgcctcgccc ttgaggtcgc gaacgtagaa ggtgtccatg
    34981 acgagccggc ggcccgttgt ttcgacgtga ccgagcacag cgccgaggac atcgacgcga
    35041 ttggcagtga gtgcaccggc gatcgccgcg aggacacccg gctcgtcggg cgcgacgatc
    35101 gcgagctcgc tgtggccctt catcgggaag cagtgaacct cgaacgcgat aggaggctgc
    35161 ttgctctgcg tgccgtgcac gagcttgatg tgacgagccg cttggcgtgc cgtgagctgt
    35221 gcgacgaggc gcgggtcgat gccgtcgacg agcgggcgag cgtcgggaac ctcgacgagc
    35281 tggacgatct tgttgcggat gctgcggtcc tcgctctcac cttcgccggc ctgcgattcg
    35341 ccgcgaaagt acgcgcgcgt acgcagcatg agatcgcgta gcagacccgc cttccatgtc
    35401 gtcaggttgt ccggcgcggt catcgcggtg tcgcacagcg tgagcagata gagcttcacg
    35461 aggcgatcat cgctgccgac gcgttcggcg aatcgcgcga tgacgtcggg atccgagagg
    35521 tcgcggcgtt gcgagagatg cgacatcgtc aggtgctgac gaacgaggaa ctcgctgact
    35581 tccatttcct cgtcggagag gccgagcctg cgggcgatcg cagcggcgac aacggcgccc
    35641 ttctcggcgt ggcctttgcc gagcggcttg ccgacgtcgt gcagcagcgt cgccaagaac
    35701 agcgcggcgg gtcgcttgac ctcgcgccac agctccgttg cgagcgggtg ctcctcagcg
    35761 agctcgccac gcgcgagtcg cttctgcatc gcgagtgcgt agagctggtg ctggtcgacg
    35821 gtgtagacgt gatagaggtc gtgctgcacg cggcccgtgc acggtgtcca ctcgggcatc
    35881 aacgccgaga gaatgccgag ctggtgcatc atctcgaacg ccgacggctg cgttgcatcg
    35941 cgaggatcga cgagcgcgtc gaggaacagc ttgttcgcga ccggatcggc ggcaagcgtc
    36001 gcggggtcgc gcgcgatggt ctcggcgacg agctccttcg tgtggccgta gaccggcagc
    36061 ttctcgtcga cggcgacgcg gaacatgcgc accatctcgc tcggacggtc cacgaacagg
    36121 cgcggatcgc gaatcgcgag ctcgccgttg aacgtgatga acgatgcgtc cacctgcgcg
    36181 atgcgaggct ttctgcgcgc aggaacgcgc gctgcctcga gcagccgatc ggcgacctgg
    36241 acgaccgcgc gcgcgtgcag atagtagtcg cgcatcagcg cttcgacggc gggcgccact
    36301 gccgagcgga tgtcgccctc gtgcgccttc gcatcggggt acaggctcgg agcaatcgct
    36361 tcttggatct cgaacgtgag ctggtcgaag cgtcgcttgg ccgtgagctg cacgagcgtg
    36421 cgaacgcgga gcatgaagtc gcgcgcggtc gtgagcacgt cgccttggcg gcgcgtcagg
    36481 tggcccatcg ccacgaggct ggcgatcagc gaatccgcgc cttcggggtc gacgcccggg
    36541 cgcggcggat gccaacgcac ttgcgcggcc cacagtgcag tcgctagatc gcgcagcgcg
    36601 ccgatgccct gcttgagatt cggctcgagc agatacagcg atgcgccgaa tcgatcgtgg
    36661 cgtgccttct tctcggcggc gagcatcgcg atgacctcgt tgggattgcc gcccggtgcg
    36721 agcgcagaca acgtcgcgcg caacaggtct gtcgtgagcc ggcgatcacc tgcgatgtgc
    36781 cgcgcgtcga gcagcgccgt ggcagtcgcg agatcgtcgc gagcgagccg ggccaccgct
    36841 cgtgcctctc gcagcgcatg accgatcgcg agcttttcgt cccacagcgg atagagcagg
    36901 cggtccgaca cctgcttcgc gctggcctcg tcgcgctcgt agaggatgat gaagtcgata
    36961 tcggagtagg gcgcgagctc gcggcgggac cagccgccgg tggcgacgag cgcaagcggc
    37021 gtggagatcc cagcacatgc ctgctcgaac cgcgtcgcca gcgcatgatc gtagacgtcg
    37081 gcgattgtcc gacatgccgc acttccgctg ccaactgcct tcaggcgcgg cgggagcgtt
    37141 gcgaacaccg cgtccagctc ggcgcgcaaa ctcggtgctg cggtacgcgc gtcctttgtg
    37201 gccacgcaac agatctagcg cggaccttgg ccccgcgtca cttgacgacg tgacctctgc
    37261 gcgggacgct tagatcgggt ggctactccg gcaccagcgg cgggcacaat gctcgggcgt
    37321 taccaggtcc tacgcccgtt agccaagggc ggcatggccg aggttctgct cgcgcgctcg
    37381 attggcatca agggcttcga gcgcttcgtg gtgctgaagc gcatcttgct gcagcacgag
    37441 aacgagcact cgatcgagat gttcctcgat gaagcgcgcc tggtcgccgg cctccatcac
    37501 cgcaacatcg tccaggtcca cgacatcggc gaagaagagg gcaagtactt cttcgcgatg
    37561 gagtacgtgc acggcgagga cgtgcgtgcg ctgctccgtg ccgcgaagga caagaagcag
    37621 caaatcccga tcgagcacgt gatcgcgatc gtcactgcgg ccgcgaccgg gctgcactac
    37681 gcgcacgacc agaaggggcc tgacaagaag cctctcggga tcgttcatcg cgatatctcg
    37741 cccggcaaca tcctcatcgg cttcgatggc tccgtgaagg tcgtcgactt cggcatcgcc
    37801 aagaccgagt cccagcacga gaacacgcag gccggcgaga tgaagggaaa gatcgggtac
    37861 atgtcgcccg agcagtgcca ggcgaaggac atcgatcggc gcaccgacgt gttcatgctc
    37921 ggcatcgtcc tgttcgagct gtgcacggtg cgccggctgt tcaaaggcga gacgaagttc
    37981 gacaccatgc agtcgatcgt ctccggcaac gtcccgccgc cgtcgaagtt ccgcaaggat
    38041 ctccccgagg agctcgagaa gatcatcctg aaggcactcg cgacggatcc gaacgatcgc
    38101 taccagacag ccgatgacct gcgcctcgtg ctcgaggcgt tcgcgatgcg aaaccagatc
    38161 cagatctcgg ggacgcgact gtccgactac atgaaggagc tgttcggcga gcgaccgctg
    38221 ccgtggctcg tcgaagagct gccgccgatg ccggatttgc aaatggtccg cgctcgcgat
    38281 tcgtcgccgg tcgtagcgcg cgcggcgtcg ctgacggagt caccgtcggg caacgtcgcc
    38341 gagaatccgt cggagtcgac gccgatggcg tggtcgacgc gcccggaggc caagggcaag
    38401 ccgacgtggt tctggccggt gattggtgcc gcgactctcg cgatcgtggg tgtcgtcgcg
    38461 ctgttcacag tgaattcgaa gagccagccg gcggcgaacc cgaagccgat cgtcgagaaa
    38521 cctgccgctc cgacgccgcc ggtcgcgacg cccgatgcgg gtatcgaagt tgtcgcggcg
    38581 cccgtcgacg ctgcggtcga ggtggtcgag acgcccgacg cggcggtcga agtggcgacg
    38641 accccgccga aaccgccgaa gcaaaagatt cgtcatccga agcctcctgc gggcccgaag
    38701 ccgcctagcg gcggagatga cctcgacaat ccgttcccca agtaggagct catgcacgtc
    38761 cgcctcgcca cgatcctgct cgcctccgtc gcattcgttc cactcgcggc ggccgacgat
    38821 ccattcgcgc agcgtgacga gggccaggcg ccgcaacagc caacgtcgcc cgcaccgccg
    38881 caggcgatcc cagatccgcc gaagaagccg ccgacgaagc aggcaaaagc cgacgcgaag
    38941 aagcacatca ccgagggcac gaagctctac aacacgcagc agtacgcgca ggccgccgag
    39001 cactatcgcg ccgcgtacat gctcgactcg cagcccgagt atttgtacgc ggcggcgcaa
    39061 tcacagcgcc tcgcgggcga ctgcacgaac gcgctgctct cgtacaacgc gtatctgcgc
    39121 accaatccca aagacgcgga gcgcgacaag gcacagaaga atatcgagcg ctgcgagcag
    39181 gatctgaaag acaaggacgc cgccgcgcag gccgaagctg ctcgcaaggc gcaggaggaa
    39241 gccgccgcga agcaggccgc cgacgccgcc gccgcgaagg ctgccgccga tgccgccgat
    39301 gcgaagcgtc gcgccgacga ggctgctgcc aatcgcgccg ccgaagaagc gaaggccgcg
    39361 gctgcgcgcc atgaagccgc gaagaagagc tacgtgcccg gccacatcat gctcggcgtt
    39421 ggcctcgtcg cggtcggtgg cggcatcttc ttgttccgcg acgcgcacag cacgatcgaa
    39481 gacttcaacg cgaccgcgag ctacgacgag ttcctcgcgg gccagtcggc tgccgacgac
    39541 gcacacacgc ggcagacgct cggcgtcctc acgatctcgg gcggcgtcgc gctcgtcgcc
    39601 ggtgccgcgg tgttctacgt gctccactcg cgcagcagca ccgaagtgcc agccgtcagc
    39661 gcgtcgatca cgcacgacag cgcgggcctc gtcttcagcg gcgcgttcta aggcgcctga
    39721 ccgaggctgt tcttcggcga gatgatagtc gtctgcggat tgaagtccgt ggcgttcgcg
    39781 tcggtgtccg tcgaggcatc gctgctgttc ggcttgcgcg acacgctgaa cgtcggcgag
    39841 atgttcggca gggcagtgcc ctcgaaacca ttcgcgacct gcgtgcccca gccgactttg
    39901 tcgagcggcc gcacgttggc cgtggtgctg aggctgatat agacgccgcc gttgtcgacg
    39961 agatccgcgg tcgccgagtt gtactgcgcg tcctggaagc cgtcccagcg gtccgagttg
    40021 ttgctgcccg tcgatgtgat caggaagaac ccacgtgatg ggatcgtggt gcgcgtgtac
    40081 gtcagcgcga gctgcgtgtt ggtcccgcct tgcgtgcgga catgcaggcg gacgccgagc
    40141 gtgctcaggt tcaacgccga ggccgtcggg ttgtagagct cgaggaactc atggtcggcg
    40201 ccattgccgc caccggcgtg cgtcaggatc tcgctcacga gcaagcctgc cttcacgacg
    40261 agcttcacgg tgctggtggt gttcgttccc acggcgttgg tgaccttgac gttccagccg
    40321 ccatagctcg agcgaatgcc tgacgggaag gtcgcggtga tctgcgtgcc gctgtccacg
    40381 gtgcagctcg tcgcgggcgt gctcacgggc gtcccgatgt tgttcgtatc gtcgagcgtg
    40441 acgccgctcg ggcacgagac cccagtgaac tccgagcccg tgatgatcac ggtcgccgta
    40501 cccacgttgt agaaggtggt gccattggcg ccggagccct gaacgttagt gctctgcacg
    40561 acgacattcg tgacgacagg cgtcgaaagc gcagtgcctg cgaagctcgc agtgttgacc
    40621 gtgagcggct cgttatccga cgcgcgcagg acgttgctga ccatcactgt gtacgtcgtc
    40681 gcgacctgcg cggtcgtcgt gaacgagacg gtgttgccgg acagcgaggg gttcgacaac
    40741 gtcaggcccg acacgctgta gttcgaattc gtgctcgccg gggtgacctg tggcggatcc
    40801 gagtacgtga ccgacatcgc gccgctgctc gtcgacatgg cggagatcgt gaacggcgtg
    40861 cggcctgcga aggtagccat gttgttgctg aggatcgtgc cgtcgctgcc gcgcgtgacg
    40921 ttcgagaccg tcaccgtgag cgtgccgctg acttgatccg aggtcggaat cgtcacggtg
    40981 ctgccgttca ggaccggagt gcccgtcgga acaacgccgc cgctgaacgc gtagttgctc
    41041 attgtcgtcg cttggccgga gtttggcgga gcgtcgaacg tgacgtccac gctgtgcgtg
    41101 ctcgtcgaca ctgcacttgt cacgttgaat agcgtgtggg tgaagttcgc tgcgttgacg
    41161 gagagcggga agttatcgcc cgcccgatgg acgttgttga ccgtgaccgt gtagagctgc
    41221 ccggcctgcg tcgaggtcgg gatcgtgacg gtgttgccga cgaggacagg cgcgccgttc
    41281 gtcgtgaggc cgccgctgaa cgtgtagttc gcctcggtct gtgcttccgt gatgacgggc
    41341 gttgcgtcga acgtgacgct catggtgttg tgattcaccg atgccgcgct cgcgacgttg
    41401 aagccggcct tccccgtgaa ggttgcgctc gcgttcgtga gcggctcgtt gtcgccggaa
    41461 cgcagaacgt tcgcgacgga gatcgtgtac gacgagcctg ccgattgcgt cgaggtcgtg
    41521 agcgtgacgg tgttgccact gacgactggc gtgccgctga ggctcagcga gcctgccgtg
    41581 atcatgtagt tgccgagcgt cgtcgcgcct gtgccgggcg tggcactgaa cgtgacgctg
    41641 aggctcgtgt tgccgacaga ggtcgcgccg gtgacattga atgcggagcg gcccgtgaag
    41701 tttgccgtct tcacggtcag cgcctcgccg tcggtacggg tgacgttgtt gaccgtgacg
    41761 gtatagttag tgccgccggt ctgcgccgac gtattgaccg tgaccgtgtt gccggtcccc
    41821 ggataattga cgctcgtcac cgcgaggccg ccggacaaca cgtagttgct ggcggtgttc
    41881 gccatgcccg agacgggcgt gccgtcgaac gtgacggtga tgcgagtgtt gagcgtcgcc
    41941 gccgcgccgg tcacgttgaa tggcaggcgc cctgtaaacg tcgcggtctt gttcgtcagc
    42001 ggttcgacgt cgccggtccg ggtcacgttg tcgaccgtga gcgtgtacga cgtgctcgac
    42061 tgcgccgcgg tcgtgagggt gacgacattg ccggtgcctg gatacgccgc tgccgaaact
    42121 gcgaggccgg gaatcgagta gttcgccgcc gtcgtggcac cggcgcccgg cggcccgctg
    42181 aacgtcacgg ccaccgacgt gctggtcacc ggagttgccg acgagacgtt gaatggagtg
    42241 cgccccgtga acgtgaagct gttcgtcgtg agcagctcgc cgtcgatgcc gcgcgtcacg
    42301 ttcgagacga cgaccgtgta tgactgattg gcctgcgctg ccgtcgtcag cgtcgcgacg
    42361 cttcccgtca cggacactgc tgaaatcgcg aggcccgaga tgaagtagtt gttgacgttg
    42421 cccgcttgcg ttgcattcgg tgcggccgaa aacgtcacct ggacctgcgt gctcagcgtc
    42481 gcctgcgcca tcgtgacgtt gaacggcaac cgaccgttga acgtagcgct cttcgccgtc
    42541 agcgcctcga gatcgccggt gcgcgtgacg ttgttcaccg tgagcgtgta catgccgccg
    42601 gtctgcggag ccgtcgtcag cgtcacgacg ttgcccgtgc ccgggtacgt tgcattcgag
    42661 accgtcaacg tcggaatcga gtagttcgcc gccgtcgtcg caccggcgcc gggcggaccg
    42721 tcgaaggtca ccgcaacgct cgtcgagctg atcgcgctcg cactcaccac gttgaacgac
    42781 gtgcggccgg tgaacgtcgt gctgttcgcg agcagaggct cgttgtcgcc ggagcgcgtg
    42841 acgccgctga ccgtgaccgt gtacgtgacg gccgactggg tcgcggtggt gatcgtgacg
    42901 gtgttaccgc tgatcgccgg cgtgccgctc agcgcgaggc cgttgttgac ggagtagttt
    42961 ccgagcacga cgccgttcgt tgcgctcggc gggccgtcga acgtgatgcg catcgatgtg
    43021 ctactgagcg acgtcgcact cacgacgttg aacttcggcc ggccggtgaa cgtcgccatc
    43081 ttgttcgcga gcggctcggc gtcagtcgcg cgcgtcacgt tgttcaccgt caccgtatag
    43141 gtggtcgccg ccatagcagg gacggtgagc gtgacgacgc tgccggtgcc cgggtacgac
    43201 accgccgtgg gggcgaggcc cggaatcgag tagttcgcga gcgtccctgc ctgtgtggcg
    43261 ttcggcggcg cgtcgaacgt gacttgcacg ttgttgctcg tgctcggcgc cgcgctcgcg
    43321 acgttgaacg tattcacgcc ggagaagttg cgcgccgcga tgatgagcgg ctcggcgtca
    43381 cttccgcgcg tcacgttcga gaccgtgacg gtgtacgtct ggcttgcctg cggagatgtc
    43441 gtgagcgtga ccgtgttgcc ggcgaccacg ggcacgccgc tgagcgtcag accggggatg
    43501 ctgaagttcg cgagtgtcgc ggcctgcgtc gcgttgggtg gggcatcgaa cgtgacgacc
    43561 accgtcgcgc tgttcggcgc tgctgcgctg acgacgttga agccgaggcg tccggtgaag
    43621 ctcgccgagc tcgcggtcag cgcctctgca tcgccggcgc ggacgatgtt gctgacggtg
    43681 accgtatagg acgtcgcgct ctgcggagag gtcacgagcg tcacggtgtt gccgacgacg
    43741 atcggggtgc ccgacagcgt gagccctggg atcgagtagc tcgacagcgc catcgcggtt
    43801 gccaggtccg gcgggccgct gaacgtcacc tgcgcgaggc cgttcgtcgc cgatgcggca
    43861 ctgacgacct tgaactcggg acgccccttg aacgtcgcgg accccatcca cagcggcgcg
    43921 tcgtcggagg cacgcgagat gttcgagacg gtcaccgtgt acgtcgtgtc ggcttgttcg
    43981 accgtcggaa tcgtgacgat gctgccggtg agcgtcggcg gaccgacgag cgtgaggcca
    44041 tcgacggagt aattggcgag gttgaccgcc tcggtggaat cgggcgcctg gctgaagctc
    44101 actgcgatcg tgacgtgatt gatcgcggcc gcgctagcga cgatgaagtc gccgttcgga
    44161 ttgacgacgc actcgcccga gaggtcgtcg cccgcagact cgccgtatcg cttgccggag
    44221 ggacagcctc gatcatcgaa gctgcagaac ccggtcgact cgcacgtgcc tacctgaccc
    44281 gcgtgcgtgc actgatccga ggtcgcgcat tgaaacgctt cgcgaggctc tgcgcacccg
    44341 acgagaacga ccgcgacaca gaggacgagc gccctcgtaa tggtccccac cttattatta
    44401 gcggctgtgc gcgccgccga gtcaaggctt cagggcttcg gggacggtgg gattccgacg
    44461 acatttgtca cttcgtcgtc gtcgatctct ggcggcggtg ggcagctgcc gcgagcggtg
    44521 cggatgcgcg acagcgtcgg aatctggtcg tgcggagggc tgaatgcagg cgcgagccat
    44581 gcggtgccct cgtcgaacga gaagatgttc ggcgatccgg gatccgtgcg gtcgtcgatc
    44641 tcgacgggca ggatgtcgtc ctccgccgca acatccatgt cgctcacgtc gatgtcgaca
    44701 tcgtcgacga tcggcgcggc ttggatcggc ggtggcttcg aggcgcgtgc tgcacgcgtg
    44761 ccggtcgcgc cgagcgcatc ggcgacgtac tggtcgatct tgtcctcgag gccgcggggg
    44821 agactcatgg ctactacggt ttcgctccgt tcgtgatcca gagcaagaag agcttgtagt
    44881 cggcgtcgtt cacatcgatg aacgtggcgt tgggatgatc ttgcgccgtc ggtggctgct
    44941 cgtacaaggg gcgtttgagt aacaatgaat cggcgggcgt cgtcgtgttg atgacgccgg
    45001 tcgcggcctt catgtttgcg agcacggtcg ctgcgggatc gtcgtacttg aggacggcgg
    45061 ctgggccact gagcgtgtga cagttcgcgc aaccaagtcc gccctgcgcc gcgcgctgca
    45121 gcttcggata gatgtgcgtc gcgaacgaag gatcggtgac gccgttgccc atgccgggac
    45181 cttgcatgcc gcccgagagt gcgagcgttg caccgtcggc gatcgtcgtc agcgacgtcg
    45241 tgttcgtgac cggttgattc tgcgcattct ggtacgtgac cgagagcgtg tacgtgccgg
    45301 gcggaacatc gaagtaggcg atccgcgcgc ggccaccgaa agcggcgacg gtggtggcgg
    45361 ccatgtcgac gttgccggcc gagttgtacg cgtacgggcc tttcactccg gtgacgacta
    45421 cgttgttgcc gtcgagcagc tggacgctcg cgagcggaac ctcgagcgcg gtgttgttgt
    45481 tgcggcggag ctcggcggtc acgaacgcgg tccccgccac gggcgtcggc gagttgatcg
    45541 acgtgtactg gttctggatg tcttggacgg acatcaagta gacgtcttgc gacagcgcca
    45601 tgtcggacac ggtgatcggc gtgtttcgcg tgttgcgata tccggtcttc gagccgacga
    45661 cgtagaactt gctgccgacc gcgacctcga ccatgtacgc gccatcggtg gcgctcgtca
    45721 tcgtggcttt cggctcgagg ccatcggtct cgagagctgc atcgcccagc gcgaccgcgc
    45781 cgaagtaatc gagcgctttt ccgctgagca cgaggccggt cggagtgacg tcggcgtcga
    45841 catcagcgtc cggcgtggcg ttctgatctg gcggcgccgt accgggacac ccggtgaccg
    45901 ccagcagagt aaatagacta ggtacgatgc gcatgaccca ctcccccgaa cggaaactcc
    45961 aatgaccatg ccagggtaac gcagtgaaac cctaggagtg caaacatcgt tcactgggac
    46021 tcgatccctc gggggtgggg gtcgactcgc caggtgcgtt gccaggatca gaccggtcat
    46081 ctaaggtgcg ccatgctcga ggaacctctt cgagaagccc tcaccttcga cgacgtcctc
    46141 ctcgtgccct cgtacagcga ggtgatcccg cgagacgttg acgtcaccac gcggctcacg
    46201 ccgctcatcc cgctgcgcat gccgctcgtc agctcggcga tggacaccgt caccgaatca
    46261 gcgacggcga tccgcatggc tcgcgaaggt ggcatggggt tcatccacaa gaacctctcg
    46321 atcgaagacc aggcgcgcga agtccaccgc gtgaagaaag cgcagtcggg catcgtcgtc
    46381 gatccgctca cgatcgcacc gtcgcgcacg ctcgaagaag cgctgtcgat catgcgcgcg
    46441 aaccgcatca gcggtctgcc cgtcgtcgat gcggacaagc gccccgttgg catcctcacg
    46501 aaccgcgacg ttcgcttcga gaagcgcttc gatatccaag tcggcgaggt gatgacgaag
    46561 aacccggtga tggtgaagga gggcgtcggt atcgacgaag ccaaagacct cctccacaag
    46621 catcggatcg agaagctcct cgtcatcgac gggcagggtg cactcaaggg cctcatcacg
    46681 atccgcgata tcgagcaggc gcaggaaaat ccgctcgccg cgacagacga gcgaggtcgg
    46741 ttgcgcgtcg gtgcagcggt tggcgtcggc cccgatcgcg atgcacgcat cgaagcgctg
    46801 atcgcagcgg gctgcgatgt catctgcatc gacaccgcgc acggtcactc ggcgggcgtc
    46861 ctcgagtccg ttcgccagac gcgcaagaag ttcccgaacg tccagctcgt cgctggcaac
    46921 gttgcgacgg gcgaagcgac gaaggcgctg atcgagaccg gcgtgaacgc ggtgaaggtc
    46981 ggcgtcggcc ctggatcgat ctgcaccacg cgcatcgtcg cgggtgtcgg cgtgccgcag
    47041 ctcaccgcga tcgcggatgc ggtcgctgcc gcaaagggct cgggcgtcgc gatcatcgcc
    47101 gatggtggca tcaagttctc gggcgacgtc gcgaaggcgc tcgctgcggg cgctgacacg
    47161 gtgatgatcg gatcgctgtt cgcgggcacc gacgaggcac cgggcgaagt catcctgtac
    47221 caaggccgct cgtacaagag ctatcgcggg atgggctcga tcggcgccat gaaagagggc
    47281 tcgaaggatc gctacttcca aggcgacgtc gagagtgcgg cgaagctcgt gcccgagggc
    47341 atcgaaggcc gcgtcccgta caagggctcg ctgcgcgagt cgatctatca gctcattggc
    47401 ggcctgcgct cgtcgatggg ctacctcggc tgcgcgacga tcgcggagct gcacgcgaag
    47461 gcgaagttcg tgaagatctc gtcggcgggc ctgcgcgaga gccacgtcca cgacgtcatc
    47521 atcacgaagg aagctccgaa ctaccgggtg gagtaatgca ccaactaggc aagtcgcatc
    47581 agctcgtttt ggtcctcgat tttgggtcgc aatacacgca gctgatcgcg cgccgcattc
    47641 gcgagcaaag cgtctactgc gagatccatc cgtgcacgat ggccgcggac ctcgagaagg
    47701 ttcgcgcgat gaagccgctc ggtatcgtgc tgtcgggcgg acctagctcc gtgtacgaag
    47761 agggcgcgcc gtcgattccg gtgggcctct acgagctcgg cattccggtg ctcggcatct
    47821 gctacggcgc gcagctcacc gcgcacctgc tcggcggcaa ggtccaccca gcggagaagc
    47881 gcgagtacgg gcgcgcaacg gtgcgggtgc tccagagccc aggcgccgcg aaagagggcg
    47941 tgttcaagac ggtgaatgcc ggcgaggagc tcgcggtgtg ggcgtcgcat ggcgatcacg
    48001 tcgagggcct gccgtcgggc ttcgtccata cggccgagag cgacaactgc aagttctccg
    48061 gcttcgcgca ccacgcgcgg aagattcact gcatccggtt tcatgccgag gtcgtgcaca
    48121 cgccgcgcgg cgccgagctg ctcggcaact tcctgttcgg cgtgtgcggc gcagcgggcg
    48181 attggtcgat ggcgagcttc gtcgaagaag ccgtcgagaa gatccgcgcg caagtcggca
    48241 gcgatgg
//
LOCUS       DATLBZ010000527        21789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5895555, whole genome shotgun sequence.
ACCESSION   DATLBZ010000527 DATLBZ010000000
VERSION     DATLBZ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21789
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5895555"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VMZ53_26440"
     CDS             complement(<1..900)
                     /locus_tag="VMZ53_26440"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS32077.1"
                     /translation="MTTTSALATLVACGGGNAPELSGLSDQVAQVGTEFKLDLNGTDP
                     DGDRLTYDFKAADLEGVKDRALVTVSPSGSGVFRWTPVASDVGQHSFDFIVSDGGNDT
                     TVTITIDVKSAIGSATSPIFRQPLGTGTTIELGKAQCADLNIVVEDQDTAQVDIKMEE
                     PVIDGATLSQEDGQTAKWHWCPSRAQEGDTRYTLVLSADDGENPKTIKNYLVVLRSGT
                     TGTNCPGGAPSISHTAANQTTRLDLKPTATITDDKGLKDAPLFYYSFTNPGATQVNLS
                     TMTQLSTTKVSGSNTNGTWAPALP"
     gene            complement(1072..7464)
                     /locus_tag="VMZ53_26445"
     CDS             complement(1072..7464)
                     /locus_tag="VMZ53_26445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32078.1"
                     /translation="MSPADKPPKGPAKPFLGDEELSSELDAWDDMFDNLHAADDGAAA
                     DLNKPVMDWPAPDPQASAPARVPEPALGQPEASRTQDDMASFEQPADELDAQMTLDAA
                     IPESVPAPAASNTWEATLDDPGETDFSDVGAQGQPAALGQMLGAQPVPAFDDADVDVG
                     ETRIDVQSRRPSTGGGYADDDDEVYTSASRPNVKPAPNERALPDDPIAPPPKPSPAVV
                     KRTGPAIIRRTPLSIPIQTPPPSSPDDFGENTRIADVGEMEAKQAESRRADARSKAPT
                     APPPMSFTPAYEPAASPDDDYEIEIGATNEDAAAVPEPVAPRRTVANVIRRPAGAPAP
                     RRESEPIIEVSRPSRPSMPAGEDDFSDVAAAVGASEETAFDDLPHTRAPSKRPPPPPE
                     PSPEPIEEEDASIDDAAETKMIHADIDMDRRDDAYVPMGAGLADEDLEVPVETGPRAT
                     SVEEMYVEEEVAQHEDDFAADDSAAMGARADERPPGLMDLYPRVKRPTSVPVLGEVRE
                     ARDESQQAPDVAAAKPRFQTTPAAYGEETPDIEPVIDPETIGADSQWPEQVQPLASSA
                     LDEESAGTLLVYERELPTVDEPTAAATLRIEAGRLCERLGDSDRARSHYDAALLADPR
                     ATAALRGLRRIARASGDLAEATRQLDAEIAVAGALERRPLAHYRVDLLMASGEHDMAR
                     VAVGELLDAAPSDVRALLAQLELAFLDGRADEFGSALEQLAHAVTDNELRAAVQQARG
                     MLAAHHNDTAAAVTWFTAAAESDPGSLGARLGAIREAAAQGNGFAAAQALLDLARGVV
                     ETDPATAAAIAMRAQHWAGGETGTAASELAAGVAAGDPLVARVTAETALAALDPENPA
                     PAAAALLGWAATEAPAAERAFAAARAAELDPANGATVWAQVLQLDPGDDYAAAQLRTS
                     FVAAEQTQSAIEVDLGVAVDVERERARLRAAFGLVAQGQLDEAIGLLQQGHAARPHSL
                     ALAEALAETLAAAGRWSERAKLLGELATTPGEQLDRDVAQLRSALAWEEAVGAASSVE
                     NPDPDEVQRATAAALGAWEKVLETSPSGTPIAHAAALVLAARLGDRDVLAEVLTRAQA
                     AERSPWGAVSLALRRARLIASEDPARVDAMLSEVQPQLDDPRRTVALLMAAARRGDVG
                     DAATTLEERANVLGETSEAAALRLRAAQLALDANDAMRATGLLRRVEKALPGVSAVSD
                     LLNAARRRAGDASGPVRMPKEGGGPASPDEFARVIRDADDAAARGEGASALGLYQTAL
                     EMRPGDPLAAVPLIRVATQLREPEPIAALALAQLKVAEATGDAAAKAEAYELLAHIDK
                     ELRSDAGSAQIALESASQADPSRIDLMHRLEREYTVGDQIGELLRLRRAELAQIPPEL
                     ARDRAAMIMDTAGLAERDKRPDAELTELYRDTLGADAKHRLALLYLESIVRRGGASDD
                     LAKLEEQIANYFEGDPRTQAAFYTRAGETLAELGQIDAAVQKFMKAEEVMPGHVPALE
                     GWRAAALKGQLWIDVAEAANRQAAIVSEPEQKAALYHFAGVVLMDKALVGEQAMVAFQ
                     RALDADPGHRDSFLRQRILLEEDANHDELANLLAKRLEHEPAGKQRVEIQRALADLMR
                     NFLTDRDGAKKHYREILTDDPNDLRAHAAVADIAWEQGAWQEAADALISRARLERDPD
                     ILKTLCFRLGLIYADRLIDVPMALKAFQRALTYQPDDEATLVRLADLATQAGEWKLAL
                     GACERLVKNETDPDKRAQHLHRVAKIFRQGFGDMKRAERALNLALDGAPTNDEALSEL
                     VRFYRDSNDMVSVRVHLNRVTGTMRVRAQQDPKDGVAYRVISRAMSARAAVGVDGSLP
                     IARTAAEIATLLGAGEEPERMLLGEPVRVDLAPLVRAEADEVLFPRSVQTELRQLFML
                     LGDRIAKHVGVDLRAYGVARGDRLRARDSSVASHAQDVATGLGFGEIDVYVSAKVPYA
                     MVAEPTSPASLVIGQAIAQMDSRAIRFAAGAALKLAQTQLAIPARLSSDDLGILVVGL
                     LRMFQDKFPTGGLDEGAIAAQTQKLKRLIPTSLATELRPFAFAVDASQWSPLEISRDL
                     KVAGLRAGLVAGGSLLAGLNILAAQAGTDVPSFLADPVAQGLITFALSEDHAALTR"
     gene            complement(7461..13100)
                     /locus_tag="VMZ53_26450"
     CDS             complement(7461..13100)
                     /locus_tag="VMZ53_26450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32079.1"
                     /translation="MSTDDDDDEGQALPEPPPRAQSEPSIDIPIEEDRPHRLQSAPSI
                     LVDDAIPRPIGEGGGNAESNVEPTAEPKSGDARPQRLQSEPTVDVDMSDDAVEEISKV
                     TPLPEPDVAKPAAVRPPAIPPISRMTPPPMPPRIPPVKPPAIPTLKGPPPKPPAIPSP
                     RASQPSVVVSIPPVGTPQPRTRPPTAPPKPKLPPAAEPASHDSQPKMSAEDMLSGGDM
                     SPLYGGVEVGTETPNIVDDQPLEAHLESPTVVDKAIAQLGDAGGEQRAEQLAKELDRT
                     ELNEPTQAAYVAYELGELYERRLADEGRAVKAFGRALNLDPSLRPNLWAIRRVFYKRG
                     LWPNLQKLVDAEVDYARDDHERADLLLEKARVSAHRLSEIDEARQALDEAVRIAPQHQ
                     GVLLELERVVARSGDVPALLDVWDRLADAVEQPARKIAYHLEVGRAAAAGGNVPRALE
                     AFDKAAALAVATPSAERVARERLRVVEEHGTPDDVAAAIDGLATVLLAAFGPAGPASE
                     SGTAAAGERPTRAAALRLELVALRRRQAQLARAEQPEKAWEYLQQALALSPGEAIVLA
                     DLTELAEQLGRYEDLAELVQNWQAVEGDPSRAMTLSIRRADALLRGGQRDQARALLAS
                     LEATAPGFIVLTSAAERDALAGKDPQALASTYLAAAHAALLGTWLGPGQDPKPDPDAA
                     SALFVQAAELLAYEVGTPEAVDEARMLLGKALEAQPKQIAAFEALVELDDATGNLVEG
                     LTRLRTAIDTVSGEDKRALVERAVRLARSHGDLETMLSLQRELIALAPDEIALRWRLE
                     STLATLGRDEERADLLVKLAADETDATGRGTALLAAARLRERSGAVEAATELYRQVLA
                     LWPEDTFARESLLDLLRAQERWQELVSERRAEAKALPDGPAARRALREAAWVLEARLD
                     DAAQAAQLYEDWLARTPDDRFALEGVARVRGKLNNRAGEADARGTLAEAEPTANSIWL
                     HARSLERAGRFDDAAEQYRSLIGSEEPSIAATNAALALGDLAASRADTVMRVEATAAL
                     AGRTADSRLGAALAEDSGWMYALVLEDFDRAAQSFEAAIALDPMRRGAQLGAALVAAR
                     RGDTAALAHAFEGLATSVQMPEAAAALLLRGAAMAAAGGDLDLANQRVQHARNAAPDD
                     TSALLVTAEIGTMPHVDAADAFAAVDPLLARAEVLEMRSALADDSAARTSWELDRAEA
                     LELAGRMREAGAVVAAVLKTRADDLRALSALHRMANRVGDKATAASAAYSLARQIGDR
                     AAKLELLREAASVFDGPGLPHNTDYAVSAFKRILASDPGAPEFDRVLELLRERADVRT
                     LIQLITERLTWHEAEGHAADTTVPLLLERATVLHGLGDTEAAMNDLDTLLDRAQSHVE
                     ALRFRADLAFNAGDVDAAVKLWRRYLAAETRPNRKGEIELQLSQVLAENTNDIGGAIE
                     QLERVVEANPEDTQLRERLLGLCLRANDWERAVRELRALARVRPTPIERAREELRLGL
                     MLRDRIGDRGQARLALDRARTLDPLNLDVVRELADLLEPAARAQVLATTAESFRTSIR
                     ENPRNAALYERLAQVNAWEADVDARWIALVAVEAVGTPSVDQRQVLAQGRASLVAPQR
                     VKLDDLARKSLRGDLGGPLHDLWRAIAPSVQVATGVDVGKLGFARGDKLAQKKLGDKY
                     EPLATALACFGLEDVEIYISAQRAGFARALAAETPILCVGADVAAASTPHNRFILGRA
                     VATLAEGVATLSELRDGELPWTIAAALRAADAQVPPALAELVVNDDTSVGERAKVLKK
                     ELSRKAKQVITSIAQTKSGELGNVDAFKRAALAVGHRTGLVWASDLAIALSVLDVGRG
                     GRAITDSPAALDLAAWSVSDDHLRLREKLGLVLKGSR"
     gene            13303..14838
                     /gene="hutH"
                     /locus_tag="VMZ53_26455"
     CDS             13303..14838
                     /gene="hutH"
                     /locus_tag="VMZ53_26455"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HUS32080.1"
                     /translation="MTTPFRLGQDVLSLEVLAQVARGGRRVEIAPGALAAMGRSRAVV
                     DAVVAGGDAAPAVYGVNTGFGALAEVRISAAEVTRLQQNLVRSHSSGVGQPLPRDAVR
                     GMMLLRAAVLATGRSGARALCCERLCDLLNAGVHPEIPARGSVGASGDLAPLAHLALG
                     MIGEGRAEYRGEWLPAADALKRADIAPLVLQAKEGLTLLNGTQHMTAIGGLTILDAEE
                     TCRVGDIAGALSLEALKGTVRAFDERVVGARPHPGQIEVARFLRALLVDSEIAESHKN
                     CGKVQDPYSLRCMPQVHGASRDVIAFARTVLEREAMSSTDNPLVIEDEMISGGNFHGQ
                     PVALALDAAAMAVAELANISERRVEQLVNPHLSSGLPPFLAPDSGLNSGFMIAQVAAA
                     SLVSENKVLAHPASVDSIPSSAGREDHVSMGATAALKLQTIHDHVRTVLAIELICGAQ
                     GIDLRRPLTTTKPLEAVHAAIRAKVPAMMVDRPIADDIRAVRAMIDDGSLIEAARSAG
                     AHA"
     gene            14884..15483
                     /locus_tag="VMZ53_26460"
     CDS             14884..15483
                     /locus_tag="VMZ53_26460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32081.1"
                     /translation="MRFALALVAVAVPAIAHADGEAMPTDDAAGDATRTDDPAANRIN
                     LRVGNATSDSTGRPTICVDVRVIRGFAAETCGTGQGVIHDQAGTELAHFRGTFSFLSR
                     RTSTGTAKLRGGLGWAELQVGVDHPGFHFGEPDTVDRGSVAGPEAVVQAQYLVPLGKG
                     IEAIASLTAGVAVFANADKLVVPQSNAQPFVSGEIGFGW"
     gene            complement(15484..16743)
                     /locus_tag="VMZ53_26465"
     CDS             complement(15484..16743)
                     /locus_tag="VMZ53_26465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32082.1"
                     /translation="MKWAVVILVVAGCRLSINKWGGIPPRATDHVKVVLHDNGGGDLG
                     KRVLTADPRVEVIAFDPAVNEKQRTDCGNLSCPLALEPACKWAAQQGADYYAIAAQSS
                     AFSQTTKCVRSHTDWSKAFDKNGDSEVCDESAVTAEGTRSSFSIRLYDATTCEEVPAL
                     SQQVTSYVPGAKAESETESAMQLGALAPTKYPGFPDQTMVAPDGHIVNAPGDGQYALF
                     RARKFQGIARLHRSGTPEEHVHMLMCCEEPQPGDVLVKRGPMKLVELDVSFDTAILAS
                     ATQHQGAYGAGIHLRHRHLAGGLELGLSGDTLEGSELSAGLVSINAGWGHHITSLLHL
                     SANVDIGFAQLLQDINGESARAWTPFAMPQARAQIAVRWWYVGLDLGYAISSTVGRGD
                     WSGEGSAMAVDARLRGPLARLYAGITL"
     gene            complement(16740..17927)
                     /gene="hutI"
                     /locus_tag="VMZ53_26470"
     CDS             complement(16740..17927)
                     /gene="hutI"
                     /locus_tag="VMZ53_26470"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HUS32083.1"
                     /translation="MSELVIHNARLMTCDPARPGLGIIERGAFASEHGRITWIGANSE
                     RPSGELEIDAGGALVTPGLVDCHTHAIFAGDRANEFAMRAAGKGYLEIAAAGGGINAT
                     LGPTRNATDEELIALMDARLTLALRGGTTTLEVKSGYDLTVEGELRLLRCIARAGTEQ
                     RLVPTLLAHLVPPDRRETRDAFVQEIVERLVPQVGAQRLAASVDVYCDEGAFTLAETR
                     AILGAAKTAGLAVRGHVGQFADLGAAEMIAELGALSADHMEQISDAGITALAKAGSVA
                     VMLPGACVQLRLPVPPIAKLRAAGIPMAIASDMNPGSSNCETLPIQMWLATTHYSMSV
                     EEAWLGVTRNAAKALALDGVGTLVADAPADFVIWNCDEPAQVPYRYGTNLVDRVFIAG
                     AEQ"
     gene            complement(17924..18163)
                     /locus_tag="VMZ53_26475"
     CDS             complement(17924..18163)
                     /locus_tag="VMZ53_26475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32084.1"
                     /translation="MSESGRTCRKIDRRGKLSDEPFDIQIARDKVIVRFRGRIVRTLT
                     GADADAVRDVIDDPDKLQLVVARKTGNFKRGNERR"
     gene            18249..19121
                     /locus_tag="VMZ53_26480"
     CDS             18249..19121
                     /locus_tag="VMZ53_26480"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUS32085.1"
                     /translation="METQMTQEMDVGDRPGTKLGKGLGWFSIGLGLTELIAPRGLARF
                     IGIQDDGKTPLVMRLFGLREIAAGAMLLARPTNPLGGWNRVAGDLIDLVTMGIAMRHS
                     TSTSRNVFALANVLGVTALDVMHGVRESRRKMGNPVRRAITIGRSREDVYSFYRKLEL
                     LPKFMNWIESVEELGNGMTHWKVKTPAGTIEYDAEITEDVPGRRISWRTLPNATIPNR
                     GVVEFLDAPGDRGCEVVLEMQVAAPLGKTIASQEAMGDLRRLKQIMELGEIVKSDASI
                     HKAPHPAQPEGEMQ"
     gene            19118..20302
                     /locus_tag="VMZ53_26485"
     CDS             19118..20302
                     /locus_tag="VMZ53_26485"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HUS32086.1"
                     /translation="MKANVWMGKQDVRVENVPDPKILNARDAIIRVTSTAVCGSDLHL
                     YNGFVPTMQKGDILGHEFMGEIVEVGSGVSNLKVGDRIAVPFTIACGVCSTCKSGKTS
                     LCENSNPNAFIQEGLLGNAGSGAFGYAHMTGGYAGGQAEYVRVPFADFGGLKIPEGLT
                     DEQVLFLTDILPTGYMGAEMADIKPGSVIAVFGCGPVGQFAIASAKLLGAEKIIAIDR
                     FPYRLEMAVQKAGATHAINYEDESVVEMIKELTGGRGADGVIDAVGMEAHGHGLQFAM
                     DRAKQVAKIQLDRAVALRDAIMTCANGGTVSVIGVYAGFIDKFPMGAFMNRGLTMRAG
                     QCHVHKYMRPLLDHIMAGRLDPTFVITHPMALSEAPKAYDLFVHKEDGCMKCVLKPDL
                     LH"
     gene            complement(20299..>21789)
                     /gene="hutU"
                     /locus_tag="VMZ53_26490"
     CDS             complement(20299..>21789)
                     /gene="hutU"
                     /locus_tag="VMZ53_26490"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HUS32087.1"
                     /translation="HVIVRELRKLENDQTLLVQSGRAVGVFRTHEEAPRVLIANSNLV
                     GKWANWEHFRELEGRGLMMYGQMTAGSWIYIGSQGIVQGTFETFAAAGKTHFGSDLAG
                     RVVLSGGLGGMGGAQPLAATMAGGVFLGVEIDPTRVQRRLETRYLDELAPDLHTALKR
                     VEECKAKKEARSIAVVGNAADVYTELVRLGFAADLVTDQTSAHDPLVGYIPQGLSLAE
                     AAELRGRDPEEYVRRARVSMAVQVQAMHAMKRSGSHVFDYGNNLRAQAELGGCPKEIA
                     YSYPGFVPAYVRPLFCEGKGPFRWAALSGDPEDIRVTDEAVLDEIKDDPMLHRWMKLA
                     KERIAFQGLPARICWLGYGQRHRVGLRFNELVKTGKVKAPIVIGRDHLDCGSVASPYR
                     ETEGMKDGTDAVADWAILNALVNTAAGASWVSFHHGGGVGIGYSLHAGMVVVADGTPR
                     AARCLERVLTSDPGMGVIRHADAGYDEAITTANERGVIVPRLRADR"
BASE COUNT         3301 a         7330 c         7485 g         3673 t
ORIGIN      
        1 cggcagcgcg ggcgcccacg tgccgttcgt gttcgagccg ctgaccttcg tggtcgacag
       61 ctgcgtcatc gtcgagaggt tgacctgcgt cgcgcccggg ttcgtgaacg agtagtagaa
      121 cagcggtgcg tccttgaggc ccttgtcgtc cgtgatggtc gcggtcggct tgagatcgag
      181 gcgcgtcgtc tggttcgccg ccgtgtgcga gatgctcggc gcgccgccgg ggcagttcgt
      241 gcccgtggtg ccgctgcgca gaacgacgag gtagttcttg atcgtcttcg ggttctcgcc
      301 gtcatcggcc gagagcacga gcgtgtagcg cgtatcgcct tcttgcgcgc gcgacgggca
      361 ccagtgccac ttcgcggtct ggccgtcttc ctgcgacagc gtcgcgccat cgatgacggg
      421 ctcttccatc ttgatgtcga cctgcgcggt gtcctggtcc tcgaccacga tgttcaggtc
      481 ggcgcactgc gccttgccga gctcgatggt cgtgccggtg ccgagcggct ggcggaagat
      541 cggcgaggtc gccgagccaa tcgcgctctt cacgtcgatc gtgatcgtga cggtcgtgtc
      601 attgccgccg tcggacacga tgaagtcgaa cgagtgctgg ccgacgtcgg aagcgaccgg
      661 cgtccagcgg aacacacccg agccgcttgg cgacacggtg acgagagcgc gatccttgac
      721 gccttcgagg tctgccgcct tgaagtcata ggtcaggcgg tcgccatcgg ggtcggtgcc
      781 gttgaggtcg agcttgaact cggtgccgac ctgggcgacc tggtccgaga gcccggatag
      841 ctcgggagcg ttgccgccgc cgcaggctac tagagtagcc agggcagacg tggtggtcat
      901 ggtccaaaga cctaggctgc tggtgagttt agctaaccgc atgacgttgc tccgaggttg
      961 atccgatggc tgtcggatgc gtggtcctta gagcaacggt taggccacgt cgcgacgcgc
     1021 cacccgccaa cgaagttgtc gccgccgccg ccgctaacta ggcgtacttg cctagcgagt
     1081 cagcgcggcg tggtcttcgc tcagcgcgaa cgtgatcaat ccctgggcca ctggatccgc
     1141 caggaagctc gggacgtcgg tacctgcttg agccgccaag atgttcagcc cggcgaggag
     1201 cgatcccccg gcgacgaggc cagcgcgcaa tccggcgacc ttgaggtctc gggaaatctc
     1261 gagcggcgac cactgcgagg catccacggc gaatgcgaaa ggccgcagct cggtggccag
     1321 gctcgtcggg atcaggcgct tgagcttctg cgtttgggcg gcgatcgcgc cctcgtccag
     1381 gccgccggtc gggaacttgt cttggaacat ccggaggagc ccgaccacga ggatgccgag
     1441 gtcgtccgac gacaaccgcg ccgggatcgc gagctgggtc tgcgcgagct tgagcgcggc
     1501 gcccgcagcg aaccgaatcg cgcgcgagtc catctgcgcg atcgcctggc cgatcacgag
     1561 cgatgccggg ctcgtgggct ccgcgaccat cgcgtacggg accttcgcgg agacgtagac
     1621 gtcgatctca ccgaagccga ggcccgtcgc gacgtcctgc gcatgcgacg cgactgacga
     1681 atcacgagcg cgcagacgat cgcctcgcgc tacgccgtac gcccgcagat cgacgccgac
     1741 gtgcttcgcg atccgatcgc cgagcagcat gaacagctgg cgcagctccg tctgcacgct
     1801 gcgcgggaac agaacttcgt cggcttccgc gcgaaccagc ggcgcgaggt cgacgcgcac
     1861 gggctcgccg agcagcatcc gctccggctc ttcgccggcg cccagcagcg tcgcgatctc
     1921 ggcggcggta cgcgcaatcg gcagcgagcc atcgacgccg accgccgcac gcgcactcat
     1981 cgcgcgagag atcacgcgat acgcgacgcc gtctttcgga tcctgctgcg cacgcacgcg
     2041 catcgtgccc gtcacgcggt tgagatgaac gcgcaccgag accatgtcgt tcgagtcgcg
     2101 gtagaagcgg acgagctcgg aaagcgcctc gtcgttcgtc ggcgcaccgt cgagcgcgag
     2161 gttgagcgca cgctccgcgc gcttcatgtc gccgaagccc tggcggaaga ttttcgcgac
     2221 gcggtgcagg tgctgcgccc gtttgtcggg gtccgtctcg ttcttgacga ggcgttcgca
     2281 cgcaccgagc gcgagcttcc attctccggc ctgcgtggcg agatccgcga gccgcacgag
     2341 cgtcgcttcg tcgtcgggct ggtacgtcaa cgcgcgctgg aacgccttga gcgccatcgg
     2401 tacgtcaatg aggcggtcgg cgtagatgag accgagccgg aagcagagcg tcttcaggat
     2461 gtccggatcg cgctcgagcc gagcacgcga gatcaatgca tcggcagctt cctgccacgc
     2521 gccttgctcc cacgcgatat cggcgacggc ggcgtgcgcg cgcagatcgt ttggatcgtc
     2581 ggtgaggatc tcgcggtagt gcttcttggc gccgtcgcgg tcggtgagga agttgcgcat
     2641 gagatccgcg agtgcgcgct ggatctcgac gcgctgcttg ccagccggct cgtgctcgag
     2701 gcgcttcgcg aggaggttcg cgagctcgtc gtggttcgcg tcttcctcga ggaggatgcg
     2761 ctggcgcagg aacgaatcgc ggtggcccgg atccgcgtcg agcgcgcgtt ggaacgcgac
     2821 catcgcttgc tcgccgacga gcgccttgtc catcaggacg acgcctgcga agtgatagag
     2881 cgcagctttc tgctctggct cgctgacgat cgcggcttgg cgattcgcag cctccgcgac
     2941 gtcgatccac agctggccct tcagggcagc cgcacgccag ccttcgagcg caggaacgtg
     3001 gccgggcatc acttcctcgg ccttcatgaa tttctgcacg gcggcgtcga tctgaccgag
     3061 ctctgcgagc gtctcgcctg cgcgcgtgta gaacgcggct tgcgtgcgcg gatcgccttc
     3121 gaagtagtta gcgatttgct cctcgagctt cgcgaggtcg tcggaagcac cgccgcggcg
     3181 gacgatcgat tcgagataga gcagcgcgag gcgatgtttt gcgtcggcgc cgagcgtgtc
     3241 gcgatagagc tccgtcagct cggcgtcggg gcgcttgtcg cgctcggcga ggccagcggt
     3301 atccatgatc atcgccgcgc gatcgcgtgc aagctccggc ggaatctgtg cgagctcggc
     3361 gcgccgcagt cgcagcagct cgccgatttg atcaccgacg gtgtactcgc gctcgagccg
     3421 gtgcatcagg tcgatgcgcg acggatcggc ttgcgacgcg ctctccagcg cgatctgcgc
     3481 ggagcccgca tcactgcgca gctctttgtc gatgtgcgcg agcagctcgt acgcctcggc
     3541 cttcgcggcg gcatcgcccg tggcttcggc caccttcagc tgcgcgaggg cgagcgcggc
     3601 aatcggctcg ggctcacgca gttgcgtcgc gacccgaatc agcggcaccg cagcgagcgg
     3661 atcgccgggg cgcatctcga gcgccgtttg atagaggccg agcgcgctgg cgccctcccc
     3721 tcgtgcggct gcgtcgtcgg cgtcgcgaat gacgcgcgca aactcgtcgg ggctcgcggg
     3781 tccgccgccc tctttcggca tgcggacagg gcccgacgcg tcaccggcgc gacggcgcgc
     3841 agcattgagc agatccgaaa ccgcgctgac gccggggagc gctttctcga cgcggcggag
     3901 aagtcccgtc gcgcgcatcg cgtcgttggc gtcgagcgcg agctgagccg cgcgcagtcg
     3961 cagagccgcc gcttcggacg tctcccccag aacgttggca cgctcttcga gcgtcgtcgc
     4021 tgcatcgccg acatcaccgc gccgagcggc ggccatcagt agtgcgacgg tacgccgagg
     4081 atcgtcgagc tgcggctgga cttcactgag catcgcgtcg acgcgcgctg gatcttcgct
     4141 cgcgatcaac cgggctcggc gcaacgcgag gctgacagcg ccccacggcg aacgttcggc
     4201 cgcctgtgca cgcgtgagga cttcggcgag gacatcgcga tcgccaaggc gagctgcgag
     4261 caccagcgct gccgcatgcg cgatcggtgt tcccgacggc gaggtctcca gcaccttctc
     4321 ccacgcgccg agagcagcgg cggtcgcgcg ttggacctcg tctgggtcgg gattctcgac
     4381 ggaggacgca gcgccgaccg cttcttccca cgcgagcgcg ctccgcagct gcgcgacatc
     4441 gcgatcgagc tgctcacctg gcgtggtggc gagctcgccg aggagcttcg cgcgttcact
     4501 ccagcgacct gcagccgcga gcgtttcggc gagagcttcc gcgagcgcga gcgagtgtgg
     4561 ccgtgctgcg tgaccttgtt gcaagagacc gatcgcttcg tcgagctggc cttgcgcgac
     4621 gaggccaaac gccgcacgca gccgtgcgcg ctcgcgttcg acgtccaccg cgacaccgag
     4681 gtcgacttcg atcgctgact gcgtttgctc cgcggcgacg aacgacgtgc gcagctgcgc
     4741 ggccgcatag tcatcgcctg gatcgagctg gagcacttgc gcccaaaccg tcgcgccgtt
     4801 ggcgggatcg agctccgcgg cacgcgcggc ggcaaatgcg cgctcggcag cgggtgcctc
     4861 ggtggccgcc cacccgagca aggcagccgc agcaggcgcc ggattctccg gatcgagagc
     4921 agcgagcgcc gtttccgcgg tgacgcgagc aacgagcgga tcgccagcag cgacgcccgc
     4981 ggcaagctcc gaagccgcgg ttcccgtctc accgcccgcc cagtgttgcg cgcgcatcgc
     5041 gatcgcagcg gcggtcgccg gatccgtctc gacgacacca cgcgcgagat cgagcagcgc
     5101 ctgcgccgcc gcaaagccgt tgccctgcgc tgcggcttcg cgaatcgcgc cgagtcgcgc
     5161 gccgagcgag cccgggtcgg actccgcggc cgcggtaaac caggtgacgg cggcagccgt
     5221 gtcgttgtga tgagccgcga gcatcccgcg cgcttgctga acagccgcgc gcagctcgtt
     5281 gtctgtcacg gcgtgtgcca gctgctcgag cgcgctgccg aattcgtctg cgcgtccgtc
     5341 gaggaatgcc agctcgagct gcgccaagag ggcgcgcacg tcggacggcg cagcatcgag
     5401 gagctcgcca acggcgacgc gcgccatgtc gtgctcgccg ctcgccatca gcaagtcgac
     5461 gcgatagtgg gcgagcggac ggcgctcgag cgcaccggcc accgcgatct ctgcgtcgag
     5521 ctggcgcgtt gcttcggcaa ggtcgccgct cgcgcgtgcg atccggcgga ggccacgaag
     5581 tgcggcggtc gcgcgcgggt cagcgagcaa cgccgcgtcg taatgcgagc gtgcgcggtc
     5641 ggaatcaccg agtcgctcgc acagccggcc ggcttcgatc cgcagcgtag cggcggccgt
     5701 cggttcatcg acggttggca gctcgcgctc gtacacgagc aacgtgcctg cggattcctc
     5761 gtcgagcgcg gagctcgcga gcggctgcac ttgctcgggc cattggctgt ccgcgccgat
     5821 cgtttcggga tcgatcacgg gctcgatgtc gggcgtttcc tcgccgtacg ccgccggcgt
     5881 tgtctggaag cgcggcttcg cggcagccac gtccggcgct tgctgtgact cgtcgcgcgc
     5941 ctctcggacc tcgccgagca cgggcaccga cgtcgggcgc ttcacgcgcg gatacaaatc
     6001 catcaggccg ggcggccgct catccgctcg cgcgcccatt gccgccgaat cgtcggcagc
     6061 aaaatcgtcc tcgtgctggg cgacttcttc ttcgacgtac atctcttcga cggacgtcgc
     6121 gcgcggaccg gtttcgaccg gtacctcgag gtcttcgtcg gcgagccccg cgcccatcgg
     6181 cacgtacgcg tcgtcgcgcc gatccatgtc gatgtcggca tggatcatct tcgtctcggc
     6241 ggcgtcgtcg atcgacgcgt cctcttcttc gatcggctcg ggcgaaggct ccggcggagg
     6301 cggcggtcgc ttcgacggcg cgcgcgtgtg cggcagatcg tcgaacgcgg tctcctcgct
     6361 cgcaccaacc gccgcggcga cgtcggagaa gtcgtcctcg cctgccggca tcgacgggcg
     6421 cgatggacgt gagacctcga tgatcggctc ggattcgcga cgtggcgccg gcgcgcctgc
     6481 cggacgacgg atgacgttcg cgacggtgcg gcgcggcgcg acgggctcgg gcactgccgc
     6541 ggcgtcttcg ttggtggcgc cgatctcgat ctcgtaatcg tcgtcgggcg acgcggccgg
     6601 ctcgtacgca ggtgtgaacg acatcggcgg cggcgccgtc ggtgctttcg atcgcgcgtc
     6661 cgctcgacgc gactctgctt gcttcgcttc catctcgccg acgtcggcga tgcgcgtgtt
     6721 ctcgccgaag tcgtccggtg acgagggcgg cggcgtctga atcgggatcg agagcggtgt
     6781 gcggcggatg atcgccgggc ccgtgcgctt gacgaccgcg gggctcggtt tcggcggcgg
     6841 cgcgatcgga tcgtcgggca acgcgcgctc gttcggcgcg ggcttcacgt tcgggcgcga
     6901 ggcgctcgtg tagacctcgt catcgtcgtc ggcgtatcca ccgcctgtcg acggccgccg
     6961 gctctggaca tcgatgcgag tctcgccgac gtcgacatcg gcgtcgtcga acgcgggcac
     7021 tggctgcgcg ccgagcatct ggccgagtgc cgctggttga ccctgcgcac cgacgtcgga
     7081 aaaatcggtc tcgcccggat cgtcgagcgt cgcttcccac gtgttcgacg ccgcgggcgc
     7141 gggcacggat tcgggaattg ccgcgtcgag cgtcatctgc gcgtcgagct cgtcggcggg
     7201 ctgctcgaag ctcgccatgt cgtcctgcgt gcggctcgct tcgggttggc cgagcgcagg
     7261 ctcgggcacg cgcgcaggtg cagacgcctg tggatctggc gccggccaat ccatcacggg
     7321 cttgttgaga tctgcggccg cgccgtcgtc ggcggcatgc aggttgtcga acatgtcgtc
     7381 ccacgcgtcg agctccgagg acagctcctc gtcgccgagg aacggctttg ccggaccttt
     7441 gggcggtttg tcggcgggac tcatcgcgat cctttcaaca cgaggccgag cttctcgcgc
     7501 aggcggagat gatcgtcgga gaccgaccat gcggcgaggt cgagcgctgc gggactgtcg
     7561 gtgatcgcgc ggccgcccct gccgacgtcg agcacggaga gcgcgatcgc gaggtcgctc
     7621 gcccagacga ggcccgtacg atgcccgact gcgagcgcag cgcgcttgaa cgcgtcgacg
     7681 ttgccgagct caccgctctt cgtctgcgca atgctcgtga tgacctgctt cgccttgcgc
     7741 gagagctcct tcttgaggac tttggcccgc tcaccgacgc tcgtgtcgtc gttgacgacg
     7801 agctcggcca atgcaggcgg aacctgcgca tcagcagcgc gcaaagcagc ggcgatcgtc
     7861 cacggcagct cgccatcgcg cagctccgac agagttgcca cgccctccgc gagcgtcgcg
     7921 acggcgcgtc cgaggatgaa gcgattgtgc ggcgtcgacg cggcagcgac atcggctccg
     7981 acgcacagga tcggcgtctc ggccgcgagc gcacgagcga agccagcgcg ctgcgcgctg
     8041 atatagatct cgacatcctc gagcccgaag catgcgagcg cggtcgcgag cggctcgtac
     8101 ttgtcgccga gcttcttctg cgcgagcttg tcgccgcgcg cgaagccgag cttgccgacg
     8161 tcgacgcccg tcgcaacttg gaccgacgga gcaatcgcgc gccagagatc atgcagcgga
     8221 cctccgaggt cgccgcgcag tgacttgcga gcgagatcgt cgagcttcac gcgctgcggc
     8281 gcgacgaggc ttgcgcgacc ctgcgcgagc acctgacgct gatcgaccga cggcgtgccg
     8341 accgcttcga ccgcgacgag cgcgatccag cgcgcatcga cgtcggcttc ccacgcgttg
     8401 acctgggcaa gcctctcgta caacgcggcg ttgcgcgggt tctcgcgaat ggaggtgcgg
     8461 aagctctcgg cagttgtcgc gagcacctgt gcacgcgcgg cgggctcgag gagatcagcc
     8521 agctcgcgga cgacgtcgag gttcagcgga tcgagcgtgc gtgcgcgatc gagtgcgagg
     8581 cgcgcttgcc cgcggtcgcc aatgcgatcg cgaagcatga ggccaaggcg cagctcttcg
     8641 cgtgcgcgct cgatcggcgt tgggcgcact cgcgccagcg cacgcagctc gcgaacggca
     8701 cgctcccagt cgttggcgcg aaggcacagc cccagcaagc gctcgcgaag ctgcgtgtcc
     8761 tcgggatttg cctcgacgac gcgctcgagc tgctcgatcg caccgccgat gtcgttcgtg
     8821 ttctcggcga gcacctgcga aagctgcagc tcgatctcgc ctttgcggtt ggggcgcgtc
     8881 tcggcagcga gatagcgacg ccacagcttc acggccgcgt cgacatcacc agcgttgaac
     8941 gccaggtccg cgcggaatct cagcgcctcg acatgcgatt gcgcgcgatc gagcaacgtg
     9001 tcgaggtcgt tcatcgcggc ctcggtatcg ccgagcccgt gcagcaccgt cgcgcgctcg
     9061 agcaacaacg gcaccgtcgt gtccgcggcg tggccttcgg cttcgtgcca cgtgagtcgt
     9121 tcggtgatca gctggatgag cgtgcggacg tcagcgcgct cgcgcagcag ctcgaggacg
     9181 cgatcgaatt ccggcgctcc cggatcgctc gcgaggatgc gcttgaatgc ggacaccgca
     9241 tagtcggtgt tgtgcggcag ccctggaccg tcgaagacac tcgcggcttc gcgaagcagc
     9301 tcgagcttgg ccgcgcgatc gccgatctgg cgagcgagcg agtacgcggc ggatgcggcc
     9361 gtcgccttat cgccgacgcg gttcgccatg cgatgcaggg ccgagagcgc gcgcagatca
     9421 tcggcgcgcg tctttagtac cgcggcgacg acggcgccgg cctcgcgcat gcggcctgcg
     9481 agctcgagcg cttctgcgcg atccagctcc cacgacgtgc gcgccgcgga gtcgtcggcg
     9541 agtgcgctcc gcatctcgag cacttcggcg cgcgcgagca gtggatcgac cgccgcgaac
     9601 gcgtcggcgg catcgacgtg tggcatcgtc ccgatttcgg cggttacgag gagcgcgctc
     9661 gtgtcatcgg gcgccgcatt gcgcgcgtgc tgcacgcgct ggttcgcgag atcgagatcg
     9721 ccgccggcgg ctgccatcgc ggcgccacgg agcaatagtg cagcagcggc ttcgggcatc
     9781 tgcaccgagg tcgcgagacc ttcgaacgca tgcgcgagcg cagcggtgtc gccgcgacgt
     9841 gcagcaacga gcgcagcgcc gagctgtgcg ccacgccgca tcggatcgag cgcgatcgcc
     9901 gcttcgaacg attgcgccgc gcgatcgaag tcttcgagca cgagcgcgta catccagccg
     9961 ctgtcctcgg caagcgcagc accgagccgc gagtccgccg tccgaccggc aagcgccgcc
    10021 gtcgcctcga cgcgcatgac cgtatcggcg cgcgacgctg ctagatcgcc aagtgcgagc
    10081 gctgcattgg tcgccgcgat cgatggctct tcggatccga tcagcgagcg atactgctcg
    10141 gccgcatcgt cgaagcgacc cgcgcgttcg aggctgcgtg cgtgcagcca gatcgaattc
    10201 gcggtcggct ctgcttcggc gagcgtgccg cgcgcgtcgg cctcgcctgc ccggttgttg
    10261 agctttccgc gtacgcgagc gacaccttcg agggcgaagc gatcatccgg cgtgcgcgcg
    10321 agccaatcct cgtagagctg tgctgcttgt gcggcatcgt cgaggcgagc ttcgagcacc
    10381 cacgcggctt cgcgcagcgc gcggcgtgcg gccgggccat cgggcaacgc tttcgcttcc
    10441 gcgcgacgct cgctgacgag ctcttgccaa cgctcttgcg cgcgcagcag atcgaggagc
    10501 gactcgcgcg cgaacgtatc ttcgggccag agcgcgagga cctgacggta gagctcggtg
    10561 gccgcttcga ccgctcccga acgctcgcgc agacgcgcgg cagcgaggag cgcagtgccg
    10621 cgaccggtgg cgtcggtctc gtcggcggcg agcttgacga gaaggtccgc gcgttcttcg
    10681 tcgcgcccga gcgtggcgag cgtcgattcg agccgccaac gaagcgcgat ttcatcgggc
    10741 gccagcgcga tcagctctcg ctgcagcgac agcatcgtct cgagatcgcc gtggctgcgt
    10801 gcgagcctga ccgcgcgttc gacgagtgcg cgcttgtctt cgccggagac ggtatcgatc
    10861 gcggtgcgca ggcgcgtgag gccctcgacg agattgcccg tcgcatcatc gagctcgacg
    10921 agtgcttcga acgccgcgat ctgcttgggc tgcgcctcga gcgccttgcc gagcagcatc
    10981 cgcgcttcgt cgacggcttc tggcgtgccg acctcgtacg cgagcagctc ggcggcttgc
    11041 acgaacagcg cggacgcggc gtccggatcc ggcttgggat cctggccggg tccgagccac
    11101 gtgccgagca gtgcggcatg cgcagcggcg agataggtcg aggcgagcgc ctgcggatct
    11161 ttgccggcga gcgcgtcgcg ttcggctgca gatgtcagga cgatgaagcc cggcgccgtc
    11221 gcttcgaggg atgcaagcag tgcacgggct tgatcgcgct ggccgccacg cagcagtgca
    11281 tcggcgcggc gaatcgacag cgtcatcgcg cgagacggat cgccctcgac ggcctgccag
    11341 ttctgcacga gctcggcgag gtcctcgtag cggccgagct gttcggcgag ctccgtcaga
    11401 tctgcgagca cgatcgcttc accgggcgag agcgcgagcg cctgctgcag gtactcccac
    11461 gctttctctg gttgctccgc ccgcgccagc tgcgcttggc gacggcgcaa cgcgacgagc
    11521 tcgaggcgca acgcagccgc acgcgtcgga cgctcgccgg cggccgcggt tcccgactcc
    11581 gatgcaggtc cggcggggcc gaacgcggcg aggagaacgg tagcgagacc atcgatcgcg
    11641 gcagcaacgt cgtcgggcgt gccgtgctct tcgacgacgc gaaggcgttc gcgcgcgact
    11701 cgctccgcgc tcggagtcgc gacggcaagc gccgcggcct tgtcgaatgc ttcgagcgcg
    11761 cggggcacgt tgccgccggc ggctgctgcg cggcctacct cgaggtggta cgcgatcttt
    11821 cgcgcgggct gctcgacggc gtcggcgagc cggtcccaca cgtcgaggag cgcaggcaca
    11881 tcgccgctgc gtgcgacgac gcgctcgagc tcgaggagca ctccttgatg ctgcggtgcg
    11941 atccgcacag cctcgtcgag cgcttgccgg gcttcgtcga tctcgctcag gcgatgcgcg
    12001 ctgactcgcg ctttctcgag cagcagatcc gcgcgctcgt gatcgtcacg tgcgtagtcg
    12061 acctctgcgt cgacgagctt ctggagattt ggccacagac cgcgcttgta gaacacgcgc
    12121 cggatcgccc acaggtttgg gcgcagcgac ggatcgagat tgagcgcacg gccgaacgcc
    12181 ttcacggcgc gaccctcgtc ggcgagccgc ctctcgtaga gctcgccgag ctcgtaggcg
    12241 acgtacgcgg cttgcgtcgg ctcgttcagc tcggtgcgat cgagctcctt cgcgagctgt
    12301 tcggctctct gctcgccgcc tgcatcgccg agctgcgcga tcgccttatc gacgacggtc
    12361 ggcgactcga gatgcgcctc tagcggctga tcgtcgacga tgttcggcgt ttcggtgccg
    12421 acttccacgc cgccgtagag cgggctcatg tcgccgccgg acagcatgtc ctcggcgctc
    12481 atcttcggct gcgaatcgtg cgatgcgggt tccgcagcgg gtggcagctt tggcttcggc
    12541 ggtgcggtcg gcgggcgcgt acggggttgc ggcgtgccga cgggcggaat cgacaccacc
    12601 acggaaggtt gcgacgcgcg cggcgacgga atcgcggggg gcttcggcgg cggacccttc
    12661 agcgtcggga tggcgggcgg cttgaccggc gggatccgcg gaggcattgg cggcggcgtc
    12721 atccgcgaga tcggtgggat cgcgggcgga cggacggctg cgggcttcgc cacatccggc
    12781 tcgggcagcg gcgtgacctt gctgatctct tcgacggcgt cgtccgacat atcgacatcg
    12841 accgtcggtt cgctctgcag gcgctgcggc ctcgcatcgc cggacttcgg ctcggcggtc
    12901 ggctcgacgt tggactcggc gtttccccca ccctcgccga tcggtcgcgg gatcgcgtcg
    12961 tctacgagaa tgctcggggc gctctgcaga cgatgcggac gatcttcctc gatcggaata
    13021 tcgatgctgg gctcgctctg cgcacgcggc ggaggttcgg gcagcgcttg gccctcgtcg
    13081 tcatcgtcgt ccgtggacat gaacgcgtcg atgcgagccg cgagtgaatt cactcggtcg
    13141 ctcttggccg gatcgctggc cgatgccggg gcgccctctc ctgcgccggc gtcatcttcg
    13201 ggtggtgtac ccaacgctct agaagatcac catttgagac gtgatgtgtc aacgccgaga
    13261 gaccgctcat tccgcgcagt tggccgtgct acacatgcgc gcgtgaccac cccttttcga
    13321 ctcggtcaag acgtgctctc cctcgaagtg ctcgcccagg tagcgcgcgg cggccggcgc
    13381 gtcgagatcg cccccggcgc actcgcggcg atgggacggt cgcgcgcggt cgtcgacgcg
    13441 gtggttgcag gaggcgacgc ggcgccggcc gtgtacgggg tcaacaccgg ctttggtgca
    13501 cttgccgagg ttcgcatctc ggcggccgag gtcacgcgcc tccagcaaaa cctcgtgcgt
    13561 tcgcactcct ctggcgtcgg tcagccgctg ccacgcgacg cggtccgagg aatgatgctg
    13621 ctgcgcgccg cggtgctcgc gaccggtcgg agcggtgcgc gcgccctctg ctgcgagcgg
    13681 ttgtgcgacc tgctcaatgc gggcgtccat cccgagattc cggcgcgcgg ctcggtcggc
    13741 gcctccggcg atctggcgcc cctcgcacat ctcgcgctcg gcatgatcgg cgaaggccgc
    13801 gccgagtatc gcggcgaatg gctgcctgcc gccgacgcgt tgaagcgcgc cgatatcgcg
    13861 ccgctcgtgc tgcaggccaa ggaagggctg acgctgctca acgggacgca gcacatgacg
    13921 gcgatcggtg ggctcacgat cctcgatgcc gaggagacgt gtcgcgttgg ggacatcgcg
    13981 ggtgcgctct cgctcgaagc tctcaaggga accgtccgcg cattcgacga gcgcgtcgtc
    14041 ggtgctcgac cgcatcctgg acagatcgaa gtcgcgcggt tccttcgcgc attgctcgtc
    14101 gattccgaga tcgccgagtc gcacaagaac tgcggcaagg tgcaagaccc gtattcgttg
    14161 cgctgcatgc cacaggtgca cggcgcgagc cgcgacgtga tcgcgtttgc tcgcacggtg
    14221 ctcgagcgcg aggcgatgag ctcgaccgat aacccgctcg tgatcgagga cgagatgatt
    14281 tcgggcggca acttccacgg ccaacccgtt gcgctcgcgc tcgacgctgc agcgatggcg
    14341 gtcgccgagc tcgccaacat cagcgagcgt cgcgtcgagc agctcgtcaa tccgcacctc
    14401 tcgagcggtc tgcctccgtt cctcgcgccc gatagcggcc tcaactctgg cttcatgatc
    14461 gcgcaggtcg ccgcggcgtc gctcgtctcc gagaacaagg tgctcgcgca tccggcatcg
    14521 gtcgattcga ttccctcgag cgccggacgc gaggatcacg tctcgatggg tgcgaccgcc
    14581 gccctcaagc tccagacgat ccacgatcac gtgcgcacgg tgctcgccat cgagctgatc
    14641 tgcggtgcgc aaggcatcga cctgcgccgg ccgctcacca ccacgaagcc gctcgaagcg
    14701 gtgcacgcgg cgattcgcgc gaaggttccc gcgatgatgg tcgaccggcc gatcgccgac
    14761 gacatccgcg cggttcgcgc gatgatcgat gatggctcgc tgatcgaggc tgctcgttcg
    14821 gcgggcgcgc acgcgtgacg ttcgccgcaa gcgttcgcag ctgacccgcg ctaggctcgg
    14881 cccatgcgtt tcgccctcgc gctcgtcgca gtcgcggtcc ccgcgatcgc gcacgccgat
    14941 ggcgaggcga tgccaaccga cgacgctgcg ggcgacgcga cgcgcaccga cgatcccgcc
    15001 gccaatcgca tcaacctgcg cgtcgggaac gcgacgagcg attcgaccgg tcgcccgacg
    15061 atctgcgtcg acgtgcgcgt gatccgtgga ttcgctgcag agacctgcgg cacgggacaa
    15121 ggcgtgatcc acgatcaggc aggcaccgag ctcgcgcact ttcgcgggac gttctcgttc
    15181 ttgtcgcgtc gaacgtcgac gggaacggcg aagctgcgcg gcggccttgg ctgggccgag
    15241 ctgcaggtgg gcgtcgatca tccgggcttt cacttcggcg agcccgatac ggtcgatcgc
    15301 ggatccgtcg cggggcccga agcggtcgtt caggcgcaat atctcgtgcc gctcggcaag
    15361 ggcatcgagg caatcgcgtc gctaaccgca ggcgtcgccg tgttcgcgaa tgcagacaag
    15421 ctcgtcgtgc cgcagagcaa cgcgcagccg ttcgtatcgg gcgaaatcgg cttcggctgg
    15481 tagctacagc gtgatgccgg cgtagagccg agcaagcggg ccgcgcagcc gggcatcgac
    15541 ggccatcgcc gagccctcgc cgctccagtc gccgcggccg accgtcgacg aaatcgcgta
    15601 tccgagatcg agccccacgt accaccagcg caccgcgatc tgcgcgcgtg cttgcggcat
    15661 cgcgaatggg gtccacgcgc gcgccgactc gccgttgatg tcttgcagga gctgcgcgaa
    15721 tccaatgtcg acgtttgccg agagatgcag gagcgaggtg atgtgatgcc cccatcctgc
    15781 attgatcgag acgaggcccg cactgagctc gctgccctcg agcgtgtcac cgctgagccc
    15841 gagctcgagg ccacccgcga ggtggcgatg acgcagatgg atgccggcgc cgtacgcgcc
    15901 ttgatgctga gtcgcgctgg caaggatcgc ggtgtcgaac gaaacgtcca gctcgacgag
    15961 cttcatcggc ccgcgcttca cgagcacgtc gccgggctgc ggctcttcac agcacatcag
    16021 catgtggaca tgctcctcgg gcgtgccgct gcgatgaagg cgcgcgatgc cttggaattt
    16081 gcgcgcgcga aacagcgcgt attggccatc gccgggcgcg ttcacgatgt ggccgtcggg
    16141 cgcgaccatc gtttgatcgg ggaagcccgg atacttcgtc ggggcgagcg cgccgagctg
    16201 catcgccgac tcggtctcgc tctccgcctt ggcgccgggc acgtagctcg tcacttgctg
    16261 cgacagcgcc ggcacttctt cgcaggtcgt cgcatcgtag aggcggatcg agaacgagct
    16321 ccgcgtccct tccgcggtca cggccgactc gtcgcagact tcggaatcgc cgttcttgtc
    16381 gaacgccttc gaccagtccg tgtgcgagcg cacgcacttc gtcgtctgcg agaacgcgct
    16441 gctctgcgcc gcgatggcgt agtaatcggc accctgctga gcggcccact tgcacgcggg
    16501 ctcgagcgcg agcgggcacg agaggttgcc gcaatcggtg cgttgcttct cgttgaccgc
    16561 cggatcgaac gcgatcacct cgacgcgcgg gtcggcagtg agcacgcgtt tgccgaggtc
    16621 gccgccgccg ttgtcatgga gcacgacctt gacgtgatcg gtggcgcgcg gcgggatgcc
    16681 accccacttg ttgatcgaga gccggcagcc ggcgacgacg agaatgacga cagcccactt
    16741 cattgctcgg cgccggcgat gaacacgcga tcgacgagat tggtcccgta gcgatacggc
    16801 acctgcgccg gctcgtcgca attccagatc acgaagtcgg ccggtgcgtc cgcgacgagc
    16861 gtgcccacgc cgtcgagcgc gagcgccttc gccgcgttgc gcgtcacgcc gagccacgct
    16921 tcttcgacgg acatgctgta gtgcgtggtc gcgagccaca tctggatggg cagcgtctcg
    16981 cagttactcg agcccgggtt catgtcgctc gcgatcgcca tcgggatccc ggcggcgcgc
    17041 agcttcgcga tgggcggcac gggcaaccgc agttgcacgc acgcgccagg caacatcacc
    17101 gcgacgctgc cagcctttgc gagcgcagtg atgcccgcgt cgctgatctg ctccatgtga
    17161 tcggccgata gcgcaccgag ctccgcgatc atttctgcag cgccgaggtc ggcgaattgc
    17221 ccgacatgac cgcgcacggc gaggcctgcc gtctttgccg cgccgaggat cgcgcgcgtc
    17281 tcggcgagcg tgaaggcacc ttcgtcgcag tagacatcta ccgatgccgc gagccgctgc
    17341 gcgccgacct ggggaacgag tcgctcgacg atctcttgca cgaatgcgtc acgcgtttcg
    17401 cgtcgatcgg gcggcacgag gtgcgcgagc aaggtcggca ccagccgctg ctccgtgccg
    17461 gccctcgcga tgcagcgaag caaccggagc tcgccctcga cggtgaggtc gtagcctgac
    17521 ttcacttcga gcgtcgtcgt cccaccgcgc aacgcgagcg tgagccgtgc gtccatcagt
    17581 gcgatgagct cttcgtcggt cgcgttccgc gtcgggccga gtgtggcgtt gatgcctccg
    17641 ccagccgcgg cgatctcgag gtagcccttc cctgctgcgc gcatcgcgaa ctcgttcgcg
    17701 cgatcacctg cgaagattgc gtgcgtgtgg caatcgacga gacccggcgt gacgagtgcg
    17761 ccgcccgcgt cgatctcgag ctcgccgctc ggccgctcgc tattggctcc aatccacgtg
    17821 atgcggccgt gctcgctcgc gaatgcgcct cgttcgatga tcccgagacc agggcgcgca
    17881 ggatcgcagg tcatgagccg cgcgttgtga atgacaagct cgctcatcgc cgctcgttgc
    17941 cccgcttgaa attgccggtt ttgcgcgcaa cgacgagctg cagcttgtcg ggatcgtcga
    18001 tcacgtcgcg cacggcatcg gcgtccgcgc ccgtcagcgt acggacgatc cgcccgcgga
    18061 agcgcacgat gaccttgtcg cgcgcgatct ggatgtcgaa tggctcgtca gacagcttgc
    18121 cgcgtcggtc aatcttgcgg cacgtgcggc cactctcgct cacgagccag aacctaccgt
    18181 agggccacac acgtgtgtaa cccgggcacg cacgttgctg tgctgtgagt cattgaagga
    18241 gtggatccat ggagactcaa atgactcagg agatggatgt cggtgatcgt cccggcacca
    18301 agctcggaaa aggtctcggc tggttcagca tcgggctggg cctgaccgag ctcatcgcgc
    18361 cgcgcggtct tgcgcggttc atcggcatcc aggacgacgg caagacgccg ctcgtgatgc
    18421 gcctgttcgg tctgcgtgaa atcgccgcgg gcgcgatgct gctcgcgcgg ccgacgaacc
    18481 cgctcggcgg ttggaaccgt gttgcgggtg atctcatcga cctcgtgacg atgggcatcg
    18541 cgatgcgtca cagcacgagc accagccgca acgtgtttgc gctcgcaaac gtgctcggcg
    18601 tgaccgcgct ggacgtcatg cacggcgttc gcgagtcgcg tcggaagatg ggcaaccccg
    18661 tgcgtcgcgc gatcacgatc ggtcgctcgc gtgaggatgt ctacagcttc tatcgcaagc
    18721 tcgagctgct gccgaagttc atgaactgga tcgagtccgt cgaggagctc ggcaacggga
    18781 tgacgcactg gaaggtgaag acgcccgccg gcacgatcga gtacgacgcc gagattacgg
    18841 aagacgttcc gggtcgccgg atcagctggc gcacgcttcc aaacgcgacg attccgaacc
    18901 gcggcgtggt cgagttcctc gatgcgcccg gcgatcgcgg ctgtgaagtc gtcctcgaga
    18961 tgcaggtcgc ggcaccgctc ggcaagacga tcgcgagcca ggaagcgatg ggcgatctgc
    19021 gtcgcctgaa gcagatcatg gagctcggcg agatcgtgaa gtccgacgcg agcattcaca
    19081 aggctccgca tcccgcgcag ccggaaggag agatgcaatg aaggcgaacg tatggatggg
    19141 caagcaagac gttcgcgtcg agaacgtgcc cgacccgaag attctcaatg cgcgtgacgc
    19201 gatcatccgc gtgacatcga cggcggtgtg cggctccgat ctgcatctct acaacggctt
    19261 cgttccgacg atgcagaagg gcgacatcct cggccacgag ttcatgggcg aaatcgtcga
    19321 agtcggctcc ggcgtgtcga acctgaaggt cggcgaccgc atcgccgtgc cgttcacgat
    19381 cgcgtgcggc gtgtgctcga cgtgcaagag cggcaagacg tcgctgtgcg agaactcgaa
    19441 cccgaacgca ttcatccagg aaggtctgct cggaaacgcc ggctcgggcg cgtttggcta
    19501 cgcgcatatg accggcggct acgcaggcgg ccaagccgag tacgttcgcg tgccgttcgc
    19561 cgatttcggc ggcctcaaga tccccgaggg tctgaccgac gagcaggtcc tgttcctcac
    19621 ggacatcctg ccgaccggct acatgggcgc cgagatggca gacatcaagc ccggctcggt
    19681 aatcgccgtg ttcgggtgcg gcccggtcgg tcagttcgcg atcgcgagcg cgaagctgct
    19741 gggagccgag aagatcattg cgatcgatcg cttcccgtat cgcctcgaga tggccgtgca
    19801 aaaggccggt gcgacgcacg cgatcaacta cgaagacgag tccgtcgtcg agatgatcaa
    19861 ggagctaacc ggcggccgcg gcgcagacgg cgtcatcgac gcggtcggta tggaagcgca
    19921 tggccatggt ctgcagttcg cgatggatcg tgcgaagcag gtcgcgaaga tccagctcga
    19981 tcgggcagtc gcgctgcgtg acgcgatcat gacgtgcgcg aatggcggaa ccgtctcggt
    20041 gatcggcgtc tacgccggct tcatcgacaa gttcccgatg ggcgcgttca tgaaccgcgg
    20101 cctgacgatg cgcgcgggcc agtgccacgt gcacaagtac atgcggccgc tgctcgatca
    20161 catcatggcg ggtcgtctcg atccgacgtt cgtgatcacg catccgatgg cgctgtccga
    20221 agcgccaaag gcgtacgacc tgttcgtgca caaagaggac ggatgtatga agtgcgtcct
    20281 caagcctgac ctgctgcact aacgatcggc gcgcagacga ggcacgatca caccgcgttc
    20341 gttcgcggtc gtgatcgctt cgtcgtatcc ggcatcggcg tggcgaatga cgcccatgcc
    20401 cggatcgctc gtgagcacgc gctcgaggca gcgcgccgcc cgcggcgttc cgtcggcgac
    20461 gacgaccatg cccgcgtgca gcgagtagcc gataccgacg ccgccgccgt ggtggaacga
    20521 gacccagctc gcaccggcag cggtattcac gagcgcgttc aagatcgccc agtccgccac
    20581 ggcatcggtg ccgtccttca tgccttcggt ctcgcgatac ggcgaagcga cggagccgca
    20641 gtcgaggtga tcgcgaccga tcacgatcgg cgccttcacc ttgccggtct tcacgagctc
    20701 gttgaagcgc aggccgacgc gatgacgctg cccgtagccg agccagcaga tgcgcgccgg
    20761 caagccctga aacgcgatcc gctccttcgc gagcttcatc cagcgatgca gcatcggatc
    20821 gtctttgatc tcgtcgagca cagcctcgtc ggtgacgcga atgtcctcgg gatcgccgga
    20881 cagcgcggcc cagcggaacg ggcctttgcc ctcgcagaac agcggccgca cgtacgccgg
    20941 gacgaagccg ggatacgagt acgcgatctc tttggggcag ccgccgagct ccgcctgcgc
    21001 acgcaagttg ttgccgtaat cgaagacgtg cgagccggag cgcttcatcg cgtgcatcgc
    21061 ctgcacttgc accgccatcg agacgcgcgc ccgccgcacg tactcctccg gatcacgccc
    21121 gcgcagctct gcagcctcgg cgagagacag cccctgcggg atatagccga cgagcgggtc
    21181 gtgcgccgac gtctgatcgg tcacgagatc cgcggcgaag ccgagccgga cgagctcggt
    21241 atacacatcg gccgcattgc cgacgaccgc gatcgatcgc gcctctttct tcgccttgca
    21301 ctcctcgacg cgcttcaacg ccgtatgcaa atcgggcgcg agctcgtcga ggtatcgcgt
    21361 ctccaagcgc cgctgcacgc gggtcggatc gatctcgacg ccgaggaaca cgccgcccgc
    21421 catcgtcgct gcgaggggct gcgcgccacc catgccgccg aggccgccgg acagcacgac
    21481 gcgtcccgcc agatcgctgc cgaagtgggt cttgcccgcc gcggcaaacg tctcgaacgt
    21541 tccctgtacg atgccttgcg agccgatgta gatccacgag cccgcggtca tctgcccgta
    21601 catcatgagg ccgcgtccct cgagctcgcg gaagtgctcc cagttcgccc acttcccgac
    21661 gaggttcgag ttcgcgatga ggacgcgcgg cgcttcttca tgcgtccgaa acacgccgac
    21721 cgccctgccc gactgcacga gcagcgtctg atcattctcg agcttgcgca gctcgcggac
    21781 gatcacgtg
//
LOCUS       DATLBZ010000528         8768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4388689, whole genome shotgun sequence.
ACCESSION   DATLBZ010000528 DATLBZ010000000
VERSION     DATLBZ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4388689"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VMZ53_26495"
     CDS             <1..782
                     /locus_tag="VMZ53_26495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32088.1"
                     /translation="PRASTPPPVGGFTSGLSAAFSGEQPAASLGALEGGDIPLSLSSV
                     DGTDRSGPVAAAVFEPPSSGESLPASIGPAPEKPKAPEKKAKPKDEPLDLFAPPESQG
                     EQFKVQLEGETERPAAQPVERKSKPSLQVPNMMGGTAVEGGVSAPSTSKLGPLGNERV
                     RYIVGVVLAILIGFLPAHFVASSMEQGAYDELDQKVIRAQQQADTPELYAALDKTRAD
                     FLDRKEAERRNAAIIALVIWGIVGGGIAFAWFKKIPWDSFE"
     gene            complement(783..1643)
                     /gene="tatC"
                     /locus_tag="VMZ53_26500"
     CDS             complement(783..1643)
                     /gene="tatC"
                     /locus_tag="VMZ53_26500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HUS32089.1"
                     /translation="MAEPQRKKRRVVYIEDDEDEVVEEKKNDELEGGRMPFLAHLVEL
                     RDRVRKAAIFFLAATIICWLKSDEIFEWLKEPLFRVWDYKKLGEPNLVFGKLTEPFWV
                     GMSIAMWAGVFAASPFIYYQIWKFIAPGLYKRERRIGITFAVCSAAFFIAGAAFCYYF
                     VLDGLYGFLISYSETDLKPMILMQDYLDLTRDMMLAFGAVFELPLLIYFLSLIGMVTH
                     RGLWKFNRWFIVIAFVVGAVLTPSPDVVSQILMAAPMIVLYNLSIIVSWRITIGRERK
                     EAEQRAREEA"
     gene            complement(1636..2157)
                     /locus_tag="VMZ53_26505"
     CDS             complement(1636..2157)
                     /locus_tag="VMZ53_26505"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HUS32090.1"
                     /translation="MFGIGGSEIIVILVVALLFLGPDKLPDAAKTISKGIRDLKKQQR
                     ALQQQIENDEQIGGAIRDLKSALRGEPEAPKRPPVRKEPPKEVTEAALETPTIMPPGH
                     DAEGNLLPDAHDENAGHDPDDPGVPDRLKLPPTAGEPDLDVAGGKSEGDEDLANLIKP
                     ATGGVAKASKTDG"
     gene            complement(2178..2732)
                     /locus_tag="VMZ53_26510"
     CDS             complement(2178..2732)
                     /locus_tag="VMZ53_26510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32091.1"
                     /translation="MTKTPLLALLVLAACGDDPVQFSEPVGIELKAKSGDVQNSTVDA
                     SKGITTESGNPYGKFISDAKAKLGGANPSTIEVDKVTLTLGAQSTNVSALEQVLTGDV
                     YVQFLTNDTNNTFVVAHFGSPTGPGPVEGHTSFDWSQVGTADVDKMLGGSFKVVLRGP
                     AATGFETKGAEASMQLTFTFTAFE"
     gene            complement(2783..4930)
                     /locus_tag="VMZ53_26515"
     CDS             complement(2783..4930)
                     /locus_tag="VMZ53_26515"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HUS32092.1"
                     /translation="MRRVVIANRREGGWWWRLPLVTAIWLALVAPFVIAFVIVSTLRS
                     WAHDLPAVPDLDAWLAEAHQTSYIVAADGTRLAELPFNDEASVVGHRMLVSIDSLPPQ
                     VVEAVLAAEDVRFFQHHGADYRAITRAAWANYKAERVVEGASTITQQVARNLLPVEIG
                     KERSARRKVREVLLARAIERRWTKHQILAVYLNFVFLGQNAYGMAAASRAYFDKPPNE
                     LTLAESALLAGLIQAPSRLDPFHHPEAAKARRDEILARMARAHLIDEPTRAAAAASPV
                     VVRRPVPTYGTRLGWYTETVRQLVAQAYPTDIDRGGLVIETAALPALGAQLQQDAADH
                     AARWKNPQVGALLWDHQTGYVEAIVGGREPWTKQNKFNRMLQSCRQPGSAWKALVYGA
                     ALESGAITPGTALRDAPIAEYDEVTNTHWKPKSGNRFRGVVLAQDAFASSLNSPAIDV
                     FDRIGATGVINFARRLGITSELADVRPMALGASCVKPIELARVYAIIARRGWAVSPRL
                     AIRVRRGNDVLFDASVPEDPWLDSANRFDRIAATTGEDPAERLLADGEQLIAEPVAFQ
                     LADMMHDVVVRGTAHAANVGRDAAGKTGTTNDNTDAWFIGFTGRELGAVWIGFDDPST
                     KLGSQGDGAHAALPLWSRAIRAAEGDRAKIEVPGPPPQGMEAVAIDRETGLLAAPSAP
                     GATLWFRSGTQPQEVAGQVGTSPTDFGRTSREF"
     gene            complement(4927..5949)
                     /locus_tag="VMZ53_26520"
     CDS             complement(4927..5949)
                     /locus_tag="VMZ53_26520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32093.1"
                     /translation="MARRTARADRPAARAASRPARTAARPAPAVTWRDGVHLSGTSIW
                     CDARRRRDVCFLSTADRVGRGGHGQLIGTPITLALAAPDSLAGHLAVPTHRPFTLGTT
                     RLELIPSGRCLGSAALHVDLGERTVLYAGEVRMKGGIENAEVRRCDALVVAAPVGETH
                     HRFAKVDDVLEQLVSFCKRQQKAGKTPMLVVDTPLDGIEVARRLVDEGMPVSASRAIR
                     TVLPKIHSLLPAKAKHLRDFIMPPGREGAIEMRVEGERVRPREKTVTALVSARAIDLR
                     TLDAYENGFPWPFVAGRDELLAWIEQSRAKEIFVTGASADAIAKKLGAKARVLGPPRQ
                     MALFAT"
     gene            complement(5994..6644)
                     /locus_tag="VMZ53_26525"
     CDS             complement(5994..6644)
                     /locus_tag="VMZ53_26525"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS32094.1"
                     /translation="MGAVRLEIRLNSQSEWIKIFDPRDWTVFVPNEALGEDADSLDNG
                     APLRIDLNVGGWLVTLRGTVVGTRDDPPGLLVALGGTEREKINYLNGFVRGGLLNLRE
                     KRRLPIKLSVTYGAIEGPAKTFTKDINEEGVFLFTDKPLPETSQVHMLVSVPGKDQPL
                     SLVGKVSHTILAQDEEPPGMGIVFDLDEAQREHLQAVIRELEEQLLENKLPEPIIE"
     gene            complement(6665..7759)
                     /locus_tag="VMZ53_26530"
     CDS             complement(6665..7759)
                     /locus_tag="VMZ53_26530"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUS32095.1"
                     /translation="MRFLRSLLFVALTGSIALADETPPPSSDAKPADAQPENAPVASK
                     RKVPLRVVRMLPETHQVLLFDKTKGTHVVAEVGQDIEGYIVDEIEDDEVTLVSPTGAE
                     VILTAPDMTWRRRQAERRAEAKKPIVADPAPVDPYADPAEAAATEPARMAAVTAPTAA
                     PIVAGSDGVRVASASGATVTPTDVAAPADPYGGDPAVAAFFEAVGASSPAPTTAPTAP
                     ATKAAPAASKSSSDGAALGAALTGSPAPISAPSATSSAPPAAATGPTTASIKRTDIDA
                     ALGDFAGTASTFRATFTPSGLRFDDVTAGSLLSKVGLQKGDVVTNIDGQPLRSLDDAA
                     NLYARLPTARGSTIQVTRAGKPVTLRIAIQ"
     gene            complement(7922..>8768)
                     /locus_tag="VMZ53_26535"
     CDS             complement(7922..>8768)
                     /locus_tag="VMZ53_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HUS32096.1"
                     /translation="RLGTLIKSNINDLITKAEDPEKMLTQVLLEMQQQLVEAKKAVAI
                     AIADEKKLQKQYTSETDKAKEWERKAMVAVRAGDDNLARQALGRKQEHETISGQFQQQ
                     WIAQKQAVEKLKDALRLLNNKIEEAKRKKNILIARKKRAEAQQQIANTMQGLGDTSAF
                     DTFDRMAERIQLMEAEAEAGAELAGELSGDTLESKFLQLEQGGAGSEDDALSELKAKM
                     GMLEAPKGESAKQLSAGAGGGKDDGHGKSDSKSSASADTGTGLTAEDLKELEDLDLSE
                     LDKKS"
BASE COUNT         1411 a         2858 c         2922 g         1577 t
ORIGIN      
        1 agccacgcgc ttcgacgcca ccaccggtcg gcggcttcac gagcgggctg tcggcagcgt
       61 tctccggcga gcaacccgca gcgagcctag gcgcgctcga aggcggcgac atcccactgt
      121 cgctgtcgag cgtcgacggc acggacagga gtgggccggt cgccgcggcg gtgttcgaac
      181 cgccgagcag tggcgagagc ttgccggcga gcatcgggcc tgcgcccgag aagccgaagg
      241 cgcccgagaa gaaagcgaag cccaaagacg agccgctcga tctgttcgcg ccgcccgaat
      301 cgcagggcga gcagttcaag gtgcagctcg agggcgaaac ggagaggccg gcggcgcagc
      361 ccgtcgagcg caagtcgaag ccgtcgctgc aggtgccgaa catgatgggc ggtaccgccg
      421 tcgagggcgg cgtctcggcg ccgagcacga gcaagctcgg tccactcggc aacgagcgcg
      481 tgcgctatat cgtcggcgtc gtcctcgcga tcctcattgg attcttgccc gcgcacttcg
      541 tcgcgtcgtc gatggagcag ggcgcgtacg acgagctcga ccaaaaggtg atccgcgccc
      601 aacagcaagc ggacacgccg gagctctacg cggcgctcga caagacacgc gcggactttc
      661 tcgatcgcaa agaagcggag cgtcgcaacg cggcgatcat cgcgctcgtg atctggggaa
      721 tcgtcggcgg cggcatcgcc ttcgcctggt tcaagaagat tccctgggac agcttcgagt
      781 agctacgctt cttcgcgcgc ccgctgctcg gcttctttgc gttcgcgtcc gatcgtgatg
      841 cgccacgaga cgatgatcga caggttgtag agcacgatca tcggcgcagc catcaggatc
      901 tgcgagacga cgtcgggcga cggcgtgagg accgcgccga cgacgaatgc gatcacgatg
      961 aaccagcggt tgaacttcca caggccgcgg tgcgtgacca tgccgatcag cgacaggaag
     1021 tagatcagca gcggcagctc gaagacggcg ccgaatgcga gcatcatgtc gcgcgtcagg
     1081 tcgagataat cctgcatcag gatcatcggc ttcaggtcag tctcgctgta gctgatgagg
     1141 aatccgtaca gaccgtcgag gacgaagtag tagcaaaacg cggcgcccgc gatgaagaac
     1201 gctgccgagc acaccgcgaa cgtgatgccg atccggcgct cgcgcttgta gaggcccggc
     1261 gcgatgaact tccagatctg gtagtagatg aacggcgacg cggcgaagac gccggcccac
     1321 atcgcgatcg acatgccgac ccagaacggc tcggtcagct tgccgaagac gaggtttggc
     1381 tcgccgagct tcttgtagtc ccagacgcga aacagcggct ccttgagcca ctcgaagatc
     1441 tcgtcggact tcagccagca gatgatcgtg gcggcgagga agaagatggc tgctttgcga
     1501 actcggtcgc gcagctcgac gaggtgcgcg agaaacggca tgcggccgcc ctcgagctcg
     1561 tcgttcttct tttcctcgac gacctcgtct tcgtcgtctt cgatgtagac gacgcgtctc
     1621 ttcttgcgct ggggctcagc catcggtctt cgaagctttc gcgacgccac ctgtcgcggg
     1681 cttgatcagg ttcgcgaggt cttcatcgcc ttctgacttg ccgcccgcaa cgtcgagatc
     1741 gggctcgccg gcggtgggtg gcagcttcaa gcgatccggc acgccgggat catcgggatc
     1801 atggccggca ttctcgtcgt gcgcgtcggg gagcaaattg ccctcggcgt cgtggccggg
     1861 cggcatgatc gtcggcgtct cgagcgcggc ttcggtgacc tctttgggcg gttccttgcg
     1921 gaccggcggg cgcttgggcg cttcgggctc accgcggagc gcgctcttca gatcgcggat
     1981 cgcgccgccg atctgctcgt cattctcgat ctgctgctgc agcgcgcgct gctgcttctt
     2041 cagatcgcgg atgcccttgc tgatggtctt cgcggcatca ggaagcttgt cgggccccaa
     2101 gaacaggagc gcgacgacca agatcacgat gatctcgctg ccgccaatgc caaacacggc
     2161 agcagcatag catcaggtca ctcgaaggcg gtgaacgtga aggtcagctg catcgacgcc
     2221 tcagcaccct tcgtttcgaa gcctgtggct gcaggacccc gcaggaccac cttgaagctg
     2281 ccaccaagca tcttgtcgac gtcggctgtg ccaacttggc tccagtcgaa cgaggtgtgg
     2341 ccctcgacgg ggcccgggcc cgtggggctc ccaaagtgcg ccacgacgaa cgtgttgttc
     2401 gtatcgttcg tcagaaattg cacgtagacg tcgccggtga ggacctgctc gagggcgctc
     2461 acgttcgtcg actgcgcgcc gagggtcagc gtgaccttgt cgacctcgat cgtggacggg
     2521 ttggcgccac cgagcttcgc tttggcgtcg ctgatgaact tgccgtacgg gttaccgctc
     2581 tcggtcgtga tgcccttgct cgcatcgacg gtcgagtttt ggacgtcacc gctttttgcc
     2641 ttcagctcga tcccgacggg ctcggagaac tgcaccggat cgtcaccgca ggctgcaaga
     2701 acgaggagcg cgagaagtgg agtcttggtc atggctatat agacgcgagt atcgcgcaaa
     2761 atgtcgctgt acaacttctg cgctagaact ctcgcgacgt tcggccgaaa tctgtgggcg
     2821 aagtcccgac ctggcctgcc acttcttgcg gctgtgtgcc ggatcgaaac cacaacgtcg
     2881 caccaggtgc gctgggcgca gcgagcaggc ccgtttcgcg atcgatcgcg accgcttcca
     2941 tgccttgcgg cggcggacct ggaacttcga tcttggcgcg atcgccttcc gctgcgcgaa
     3001 tcgcgcgcga ccacagcggc aacgcagcgt gcgcgccgtc gccctgcgag ccgagcttcg
     3061 tcgacggatc atcaaagccg atccacaccg cgccgagctc gcgtcccgtg aagccgatga
     3121 accacgcatc ggtgttgtcg ttcgtcgtgc ccgtcttgcc cgcggcatcg cggccgacgt
     3181 ttgcggcatg cgcagtcccg cgcacgacca cgtcgtgcat catgtccgcg agctggaacg
     3241 cgacgggctc ggcgatcagc tgctctccgt cggcgaggag gcgctctgcc gggtcctcac
     3301 ccgtggtcgc ggcgatgcga tcgaagcggt ttgcggaatc gagccacggg tcttcgggga
     3361 cgctcgcgtc gaacaacacg tcgttgccgc gccgcacgcg gatcgccaac cgcggagaca
     3421 ccgcccagcc tcgtcgcgcg atgatcgcgt agacgcgcgc gagctcgatc ggcttcacgc
     3481 acgatgcgcc gagtgccatc ggccgaacgt ctgcgagctc gctcgtgatg ccgagcctgc
     3541 gcgcgaagtt gatgacgccc gtcgcgccga tgcgatcgaa cacgtcgatg gcgggggagt
     3601 tgagcgacga cgcgaacgca tcttgcgcga gcacgacgcc ccgaaagcga ttgcccgatt
     3661 tcggtttcca gtgcgtgttc gtgacttcgt cgtattcggc gatcggtgca tcgcgcagcg
     3721 cggtgcctgg cgtgatcgcg ccgctctcga gcgcagcgcc gtagacgagt gccttccacg
     3781 cagagccggg ttggcggcag ctctgcagca tgcggttgaa cttgttttgc ttcgtccagg
     3841 gctcgcgacc gccgacgatg gcttcgacgt agccagtctg gtggtcccac agcagcgcgc
     3901 caacctgcgg attcttccag cgcgcagcgt gatcggcggc gtcttgttgt agctgtgcgc
     3961 cgagcgcggg cagcgccgcc gtctcgatga cgagccctcc gcgatcgatg tccgtcggat
     4021 acgcctgcgc gacgagttgg cgaaccgtct cggtgtacca gccgaggcgc gtgccgtacg
     4081 tcggaacggg gcggcgcacg acgacaggcg atgcagcggc ggccgcacgt gtgggctcgt
     4141 cgatcaggtg cgcacgcgcc atgcgcgcga ggatctcgtc gcgtcgcgcc ttcgcggctt
     4201 cgggatggtg aaatggatcg aggcggctcg gtgcctggat caaacccgcg agcaacgcgc
     4261 tctcggccag cgtgagctcg ttcggcggct tgtcgaagta cgcgcgcgac gcagccgcca
     4321 tgccgtacgc gttctgtccg aggaagacga agttcaggta gacggcgagg atctggtgct
     4381 tcgtccaccg ccgctcgatc gcgcgcgcga gcaagacctc gcggaccttg cgcctcgcgc
     4441 tgcgctcctt gccgatctcg acgggcagca agttgcgcgc gacctgctgc gtgatcgtcg
     4501 acgcgccttc gacgacgcgc tcggctttgt agttcgccca cgcagcgcgc gtgatcgctc
     4561 gatagtccgc gccgtgatgc tggaagaagc gcacgtcttc cgcagcgagc accgcctcta
     4621 cgacctgcgg cggcaacgag tcgatcgata cgagcatgcg atgaccgacg acgctcgctt
     4681 cgtcgttgaa cgggagctcg gcgaggcgcg tgccgtcggc cgccacgatg tacgacgtct
     4741 ggtgcgcctc cgcgagccac gcgtcgaggt ccggcacggc cggtagatcg tgcgcccagc
     4801 tgcgcagcgt cgagacaatc acgaacgcga tcacaaatgg cgcgacgagc gcgagccaga
     4861 ttgcagtcac gagcggcagt cgccaccacc agccaccttc gcggcgattg gcgatgacga
     4921 ctcgtctcat gtcgcgaata atgccatttg ccgaggaggg ccgaggacgc gcgccttggc
     4981 accgagcttt ttcgcgatcg catcggcact tgcgccggtg acgaagatct ctttcgcgcg
     5041 cgactgctcg atccacgcga gaagctcgtc gcgcccggcg acaaacggcc acggaaagcc
     5101 gttctcgtag gcgtcgagcg ttcggaggtc gatcgcacgt gccgacacga gcgcggtgac
     5161 ggtcttctcg cgtggacgca cgcgctcacc ttcgacgcgc atctcgatcg cgccttcgcg
     5221 tccgggcggc atgatgaagt cgcgcagatg ctttgccttc gcgggcagca gcgaatggat
     5281 cttcggcagc accgtgcgaa tcgcgcgcga tgcggagaca ggcatgcctt cgtcgacaag
     5341 ccgcctggcg acttcgattc catcgagcgg cgtatcgacg acgagcatcg gcgtcttgcc
     5401 cgctttctgc tgccgcttgc agaagctcac gagctgctcg agcacatcgt cgactttcgc
     5461 gaaccggtga tgcgtctcac cgaccggtgc agcgacgacg agcgcatcgc atcgccgcac
     5521 ctccgcattt tcgattccgc ccttcatgcg gacctcaccg gcatacagca cggtgcgctc
     5581 gccgagatcg acgtgcagcg cagcgctgcc gagacatcgc cccgacggaa tcagctcgag
     5641 gcgcgtcgtg ccgagcgtga acggccgatg cgtcggaacc gcgagatgac cggcgagcga
     5701 atcgggcgcc gcgagcgcga gcgtgattgg cgtgccgatg agctggccgt gaccgccacg
     5761 gccgacgcga tccgcggtcg agagaaaaca gacgtcgcgt cgcctgcgcg catcgcacca
     5821 gatcgacgtg ccggacaggt gcacgccatc gcgccacgtc accgcaggcg ctgggcgagc
     5881 cgccgtacgt gccgggcgcg acgccgcgcg cgcagcgggt cgatcggcgc gggcggtacg
     5941 acgtgccacg cctcgcacgg tgacatccgc ctgccaatct cgcaacttag cggctactcg
     6001 atgatcggct cgggcagctt gttctcgagc agctgctctt cgagctctct gatcacggcc
     6061 tgcaagtgct cgcgctgcgc ttcgtcgaga tcgaacacaa tgcccatgcc cggtggctct
     6121 tcgtcttgcg cgaggatcgt gtggctgacc ttgccgacga gcgacagcgg ctgatccttg
     6181 cccggcacgc tgacgagcat gtgaacctgc gacgtctcgg ggagcggctt gtcagtgaac
     6241 agaaacacgc cctcctcgtt gatgtccttc gtgaacgtct tcgcagggcc ttcgatcgcg
     6301 ccgtatgtga cgctgagctt gatcggcaga cggcgtttct cgcgcaggtt caacaggccg
     6361 ccgcgcacga agccattcag gtagttgatc ttctcgcgtt cggtgccgcc gagggcgacg
     6421 aggaggcccg gtggatcatc gcgcgtgccg acgacggtgc cgcgcagcgt gacgagccag
     6481 ccgccgacgt tgaggtcgat gcgcagcggc gcgccgttgt cgaggctgtc ggcgtcctcg
     6541 ccgagcgctt cattcggcac gaacacggtc caatcgcggg gatcaaagat cttgatccac
     6601 tccgactggc tgttcagtcg gatctcgagc cggaccgcac ccacgcgacc atcgtaaccg
     6661 aaatttactg aatcgcgatg cgcagcgtta cgggcttgcc cgcgcgcgtc acctggatcg
     6721 tcgagccgcg tgcggtcggc agacgcgcgt agaggttcgc ggcgtcgtcg agagagcgga
     6781 gcggctggcc gtcgatgttc gtgacgacgt cacccttttg gaggccgacc ttcgagagca
     6841 gcgagcccgc ggtcacgtcg tcgaagcgca ggccgctcgg cgtgaacgtg gcgcggaacg
     6901 tcgatgcggt gccggcaaag tcgccgagcg cggcgtcgat gtccgtgcgc ttgatcgagg
     6961 ccgtcgtcgg accggtggct gcggcaggag gggccgacga cgtcgcgctg ggcgcactga
     7021 tcggggcagg cgagccggtc agtgcagcgc ccagcgcggc gccgtcagat gaagacttgg
     7081 acgctgcggg tgcagctttg gttgcaggag cagtgggcgc ggtcgtcggt gccgggctcg
     7141 acgcgccgac ggcctcgaag aacgcagcga ctgccgggtc gccgccgtac gggtcagccg
     7201 gcgcggccac gtcagtcggc gtcaccgttg caccgctggc gctggcaacg cgtacgccat
     7261 cggagccggc tacgatgggc gcagcagtcg gcgcggtcac ggcggccatc cgtgcgggct
     7321 ccgtggcagc agcctcggcc gggtcggcgt acgggtcaac cggcgcaggg tcggcgacaa
     7381 tgggcttctt cgcctcggct cggcgctcgg cctggcggcg acgccacgtc atgtcgggag
     7441 ccgtcaggat gacctcggcg ccggtcggcg agacgagcgt cacttcgtcg tcctcgatct
     7501 cgtcgacgat atagccctcg atgtcctggc cgacctcggc cacgacatgc gtgcccttgg
     7561 tcttgtcgaa aagcagaacc tggtgagtct ccggcagcat cctcaccacg cggagcggca
     7621 ccttgcgctt gctcgcgacg ggcgcgtttt ccggctgtgc atccgcgggc ttcgcgtcgc
     7681 tcgagggagg cggcgtctcg tcggcgagcg cgatcgagcc tgtcaaagcg acgaacagaa
     7741 gcgaccggag aaaccgcatg gcgggacgta ttgcaagcac cgatcccgtt caaacgcgcg
     7801 gaagcacgcg tcgatctacc ggtcctgtga atggtcgacc acaggaggtg tgcccctgtg
     7861 ggacgacttt gttgcggcat gtttctcgac gagaaacggc ctgcgaacgc gctagcgagg
     7921 tctacgactt cttgtcgagc tcggagaggt cgaggtcttc gagctctttg aggtcttccg
     7981 ccgtcaggcc ggtgccggta tctgccgacg ccgagctctt cgagtcggac ttgccgtgac
     8041 cgtcgtcctt gccgccgccg gcgcccgccg acagttgctt ggcgctctcg cccttaggcg
     8101 cttcgagcat gcccatcttc gccttgagct cggagagtgc gtcgtcctcg gagcccgcgc
     8161 cgccctgctc gagctggagg aacttcgact cgagcgtgtc gcccgacagc tcgcccgcga
     8221 gctctgcgcc ggcttccgcc tcggcttcca tcagctggat gcgctcggcc atgcgatcga
     8281 acgtgtcgaa cgccgacgtg tcgcccaggc cctgcatcgt gttcgcgatc tgctgctgcg
     8341 cctcggcgcg cttcttccgc gcgatgagga tgttcttctt gcgcttcgct tcctcgatct
     8401 tgttgttgag gaggcgcagc gcgtccttca gcttctcgac ggcttgcttc tgggcgatcc
     8461 actgctgctg gaactgacca gaaatcgtct cgtgctcctg cttgcgcccg agcgcttgac
     8521 gcgcgaggtt gtcatcacca gcgcgaacgg cgaccatcgc cttgcgctcc cactccttcg
     8581 ccttgtccgt ctctgatgtg tactgcttct ggagcttctt ctcgtcggcg atcgcgatcg
     8641 cgacggcctt cttcgcctcg acgagctgct gctgcatctc gaggaggacc tgcgtcagca
     8701 tcttctccgg atcttcggcc ttcgtgatga ggtcgttgat gttcgacttg atcagcgtcc
     8761 cgagtctg
//
LOCUS       DATLBZ010000529         8196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2926547, whole genome shotgun sequence.
ACCESSION   DATLBZ010000529 DATLBZ010000000
VERSION     DATLBZ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2926547"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VMZ53_26540"
     CDS             complement(<1..1626)
                     /locus_tag="VMZ53_26540"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HUS32097.1"
                     /translation="MARPAHAVDPVPSFRYLVTGNGRGFQVFDVSANAVKQFLERPYR
                     YLRASSSNPDGEGVVRRNLAFDTYFGVRVGGSAAWMGGRAPDSVGYVDQSNMIRSTST
                     VGGLSVSSYYVSPFGFEGNAMVMLLEVHNGTGSAQSATAFSIHNFKMGSASNPDAPDG
                     NGEAIAWDASSMTATETGPGGGVIVYAPLGGMDVSSCNANAYSAVAGGGNLTSQAACS
                     GSDQKNAFQKDLGSIPPGESRWWGVAVLFDADGNANAARTAWTNFLGGKTPEQLYTSV
                     LAEFEAWRKMPAPGLSTAETAIWRQAETVLRMGQVIEPYTDAPKRHNTGMILASLPPG
                     GWHTGWVRDATYAINALARSGHGPEAKLGLDFFLNADAGRYASYVGNQPYRISTVRYY
                     GDGQEEADYSGSPTRNIEIDGWGLFLWAARTYVDSTGDIAWLNAVTKKGDTVYDAIKS
                     GVADPLVANLESSGLAIADASIWEVHWGNRQHFLYTTATAARGFCDMATLARRAGKTD
                     DIAKYRMLAEKATLAMKTNLVDSNKVLAGSLERL"
     gene            1932..2342
                     /locus_tag="VMZ53_26545"
     CDS             1932..2342
                     /locus_tag="VMZ53_26545"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HUS32098.1"
                     /translation="MLALLVAGCRPPGYGKEPDVDAATSTVDASTQTGDAGHDATTAA
                     TCPHAFRLDGHGTDSSVWLTGSFINWAGTPQAGAIAFTLGVDGGWTGNYAFPAGSHQY
                     KYIVNGNNWILDPTNANTADDGMGHTNSLYVCVP"
     gene            complement(2339..3085)
                     /locus_tag="VMZ53_26550"
     CDS             complement(2339..3085)
                     /locus_tag="VMZ53_26550"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HUS32099.1"
                     /translation="MFARFACLLLAGCGRIAFDPLSGGGAPGDTQTGDAFGGPAGSAV
                     SLNGTGYVSLDSLCPLLVSGFTIDGWFRLGATTQIDSDTVAPIGLNSNDMVTNYTLVL
                     WDWRDGSLAYYDDVYTMRTQNPAAVPGDEWHFFSLTYVPGRAIVYADGTSFSDAQTPQ
                     TVTSPCHVSLGQELDITGPSDFMVGLIDEVSVWSVAQSPSEILATMQANVTGSEPNLV
                     GYYRFDGNGADSSGHNRTANLVAATFVAVP"
     gene            3158..3973
                     /locus_tag="VMZ53_26555"
     CDS             3158..3973
                     /locus_tag="VMZ53_26555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32100.1"
                     /translation="MRWVLAVALLAACHTSAGTGDDDDTTTDARGMGSGSGSNGGLVN
                     VFDPGITRVIVEIDYETNQQPYTGNIIGFGDTFAPTMTNVDRLFAHKKTLTIPQALAN
                     MEDIGTIPDEELTVADLAAIAQAHRQQYDVDGARTYYIVFVSGHYADGNGVNAGVLGV
                     SFGDTIAMFKDVIRTTASLTSPNSERYVEQSTLIHELAHSIGLVDNGVPMVAGHKDAT
                     HGAHCNNDQCVMYWLNEGAADATSFAVNRLITGSTILFDAACLSDVDAKSGGL"
     gene            complement(3970..5316)
                     /locus_tag="VMZ53_26560"
     CDS             complement(3970..5316)
                     /locus_tag="VMZ53_26560"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HUS32101.1"
                     /translation="MRLAVIALLLLASCVQSKSVTCGSLVCSEASVCAPSGDRCVLAI
                     QADACEGRADRDACTYTGVAMGICNGAVCYPIGCGNGLVEPEQGELCDDGNRASFDGC
                     SADCHSTEVCGDGAIDIAIGEQCDAGDANSDAPGAPCRLGCRFAGCGDGIIDPSEECD
                     DGNKLPGDGCRADCAGRWTRLPSGTYMPLRAVWGASPANVFFVGDNGTILHFDGSHVT
                     AMAAPQPPIDYYAVAGSGPTNVIAAGSERIDRFDGTSWSTLHVTTDTVWRDAWASPAS
                     GVWLVGNGNATLATQAVFAHVTPQYSGVLAISSAREATKLWSDGTKLYVTTASGAGWI
                     RTGSTWASTGGLSGIRLTGVSSTEIYTYDNLGAGLFNGSTTTPMPNSDEMGPDAHDIG
                     GLAGDVLVVGGLGKVTRFDGTTWSIAPTPSSAILYDVWSASVGQAFIAGHQGTILY"
     gene            complement(5331..6623)
                     /locus_tag="VMZ53_26565"
     CDS             complement(5331..6623)
                     /locus_tag="VMZ53_26565"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS32102.1"
                     /translation="MAGPRVIIEASPDTPLVWFDVAIRGGAAADPRGIEGLHRHAALL
                     ARRGAGSRDRQQLDETLDSLGAMIDIGVSRDAVTLSGLALSRHLDAVIDLCADILGEP
                     KFATDEHARLLRETPQVLDEIRDDDSALATRWFDWLCCPGHPYGRTSLGTETSLEQIE
                     RGIAADLWKREVVANNLVIGLAGDIDERAATKIVSRLTERIPPGGRATTHVEDTGDEA
                     ESRRGRRLIVVDKPERTQAQLRIGHLALRYGHPDTAALAVAEAVFGGMFSSRLMQEIR
                     VKRGWSYGAGCALRRSRLPHWFEIWTAAAIEHAGPCVALTLDLLADYAAQGPTDDEVD
                     FARSYLVGAMPFHIATARQRMQLAVRDAVFELPDGYTAKLPEALAQLTAADVRAACKR
                     HLRPEDAVTVAVTTAEDAQAALSAANAGPMTVVDHDEY"
     gene            complement(6623..7969)
                     /locus_tag="VMZ53_26570"
     CDS             complement(6623..7969)
                     /locus_tag="VMZ53_26570"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS32103.1"
                     /translation="MTQSSWQLEAEAPAGRGVTARRYRLSNGLGLISCIDRRAPIVAL
                     QTWFRVGSRHERPGATGMAHLFEHLMFGQTEKLPPGEFDRQVERTGGESNAATWVDWT
                     YYRLSLPARDLELGIKLEAERMQHLVLEPTPVEAERDVVTNERRERVEDDVDGWLDEQ
                     LMAHAFTAHPYRWPTIGWMEDIRALALPDIRAFYRTWYAPNNATIVCVGDFDDEQLRD
                     TIAKYYGAIPPATLPEPSKIVEPEQTRERVVRAPKPIATDRLLIGYKAPGQDDPDWAP
                     LEIVSTLLAGCPSARLHRKLVIQDESASSVDAQLTPFRDPSLLRLAVTTARGHDADEV
                     IATIDAELNALIETPPSAAEVEKAKALAETDFWSSLVDVDGKAEALGHYETALGDFRR
                     VNVLAERLTQVTTNDVARVVRAYLLPTRRTIVIAEPESEEEREDNDAEHEDDGGES"
     gene            complement(7989..>8196)
                     /locus_tag="VMZ53_26575"
     CDS             complement(7989..>8196)
                     /locus_tag="VMZ53_26575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32104.1"
                     /translation="RFDAAQIVVRGSDQYRASAGIAAWVARQLATRTTGPVGMRAPSE
                     LFRAPLALRAVADSLGLSVEPSFG"
BASE COUNT         1388 a         2701 c         2726 g         1381 t
ORIGIN      
        1 gaggcgctcg agcgagccgg cgagcacctt gttcgaatcg acgaggttcg tcttcatcgc
       61 gagcgtcgcc ttctcggcga gcatgcggta cttcgcgatg tcgtcggtct tgcccgcacg
      121 acgcgcgagc gtcgccatat cgcagaagcc acgcgcggcc gtcgcggtcg tgtagaggaa
      181 gtgctggcga ttgccccagt gaacttccca gatcgacgcg tccgcgatcg cgagcccact
      241 cgactcgaga ttggcgacga gtggatcggc gacgccgctc ttgatcgcgt cgtagacggt
      301 gtcgcccttc ttcgtgacgg cgttgagcca ggcgatgtcg cccgttgaat cgacgtacgt
      361 gcgagcggcc cacaggaaga ggccccagcc atcgatctcg atgttgcgag tcggcgagcc
      421 cgagtagtca gcctcttctt ggccgtcgcc gtagtagcga acggtcgaga tccgatacgg
      481 ctggttgccg acgtagctcg cgtagcggcc tgcatcggcg ttcaagaaga agtcgaggcc
      541 gagctttgct tccggaccgt ggcccgagcg cgcgagcgcg ttgatcgcgt acgtcgcatc
      601 gcggacccag cccgtgtgcc agccgccggg cgggaggctc gcgaggatca tgccggtgtt
      661 gtgacgcttc ggtgcgtcgg tgtacggctc gatcacctgg cccatgcgca ggaccgtttc
      721 cgcttggcgc cagatcgcgg tctccgccgt cgagaggcca ggcgccggca tcttgcgcca
      781 cgcttcgaat tcggcgagca ccgacgtgta gagctgctcc ggcgtcttgc cgccgaggaa
      841 attcgtccac gccgtgcgcg cggcgttcgc attgccgtcg gcgtcgaaca acacggcgac
      901 gccccaccag cgtgattcgc ccggcgggat cgagccgagg tctttctgga acgcgttctt
      961 ctgatcgctg ccgctgcacg cagcttgcga cgtgagattg ccgccgcccg cgaccgccga
     1021 gtacgcgttc gcattgcagc tcgacacgtc catgccgccg agcggcgcgt agacgatcac
     1081 gccaccgccg gggccggtct ccgtcgcggt catcgacgat gcgtcccacg cgatcgcttc
     1141 gccgttgcca tcgggcgcgt cggggttcga cgccgagccc atcttgaagt tgtgaatcga
     1201 gaacgcggtc gcggactgcg cgctgccggt gccgttgtgc acctcgagga gcatcaccat
     1261 cgcgttgccc tcgaagccga acggcgagac gtaatacgag ctcaccgaca ggccgccgac
     1321 ggtcgacgtc gagcggatca tgttcgactg atcgacgtag ccgaccgagt cgggcgcgcg
     1381 gccgcccatc catgctgcgc tccccccgac gcgaacgccg aagtacgtat cgaacgcgag
     1441 gttgcggcga acgacgcctt cgccatcggg gttcgacgag ctcgcgcgca ggtaccgata
     1501 cggccgctcg aggaactgct tgaccgcgtt cgcgctgacg tcgaagacct gaaaaccgcg
     1561 gccgttgccg gtcacgaggt agcggaacga cggcaccgga tcgaccgcgt gagccggccg
     1621 cgccatggcc gagatcgcca cgaccgaggc aagaggcagg aggatgcggc tccgcagcga
     1681 catgttcgcg ccgggccttt cgcatgattc atgcccacga tcgcgaggcg cggcgcgtgt
     1741 ttattcgcgt acctgcgcgc ccgcccaggt tgccaaacgc aacccaccgg cgatcgctcg
     1801 cgctgccaat cgcgacgccc agaaaggggt caggcacaag aaaacaagaa actacggagt
     1861 tacttgttta cttgtgcctg accccgttgc tggcacggtg catgaacact gcaacggcga
     1921 tgcgtctggt cgtgctcgct ctgcttgtgg ccggctgccg tccacccgga tacggcaagg
     1981 agcccgatgt cgacgcggct acctcgacgg tcgacgcatc gacgcagacg ggggacgcag
     2041 ggcacgacgc gacgaccgcg gcgacatgcc cgcacgcgtt tcggctcgat gggcacggca
     2101 ccgattcgtc ggtgtggctc acgggtagct tcatcaactg ggcggggaca ccgcaggctg
     2161 gcgcgatcgc attcacgctc ggcgtcgacg gtggctggac gggcaactac gcgttcccgg
     2221 ccggctcgca tcagtacaag tacatcgtca acggcaacaa ctggatcctc gatccgacga
     2281 acgcgaacac cgccgacgac ggcatggggc acacgaacag cctctacgtc tgcgttccct
     2341 agggcaccgc gacgaacgtc gctgcgacga gattcgccgt ccggttatgt cccgagctat
     2401 ccgcgccatt gccgtcgaag cggtagtagc cgacgagatt cggttcgctg cctgtcacgt
     2461 ttgcttgcat ggtcgcgagg atctcgctcg gtgactgcgc gacgctccag acgctgacct
     2521 cgtcgatgag cccgaccatg aagtcgcttg gaccggtgat gtcgagctcc tgaccgagcg
     2581 agacgtgaca tgggctcgtt accgtctgcg gtgtttgcgc gtcggagaag ctcgtgccgt
     2641 ccgcgtacac gatggcgcgc ccggggacat acgtgagcga gaagaagtgc cactcgtcgc
     2701 caggcacggc agcggggttc tgcgtccgca tcgtgtacac gtcgtcgtag taggcgaggg
     2761 agccgtcgcg ccagtcccac agcacgagcg tgtagttcgt caccatgtcg ttactgttga
     2821 gcccgatcgg cgcgaccgtg tcgctgtcga tctgcgtcgt cgcgccgagc cggaaccagc
     2881 catcgatcgt gaagcccgac acgagaagtg ggcacaggct gtcgaggctg acgtagcccg
     2941 tgccgttgag cgagaccgcg ctgcccgccg gaccgccgaa cgcatcaccg gtctgcgtgt
     3001 cgccaggagc tccgccgccg gagagcggat cgaacgcgat gcgaccgcag ccggcaagca
     3061 gcaaacatgc gaaacgcgcg aacacgagtg aggatcgtaa cactggctca cgcacgcctg
     3121 acgatcggaa cgattccagg acgcgtacac tgagcgtatg cgctgggtgc tcgctgtcgc
     3181 gctcttggcc gcgtgtcaca cgtcggctgg caccggcgat gacgacgaca ccacgacgga
     3241 cgcgcgtggc atgggctccg gctcgggctc gaacggcggc ctcgtgaacg tgttcgaccc
     3301 cggcatcacg cgcgtgatcg tcgagatcga ttacgagacg aaccagcagc cgtacaccgg
     3361 caacatcatc ggctttggcg acacgttcgc gccgacgatg acgaacgtcg atcggctgtt
     3421 cgcgcacaag aagacgctga cgattccgca ggcgctcgcc aacatggaag acatcggcac
     3481 catccccgat gaagagctca ccgtcgcgga tctcgcggcg atcgcacagg cgcaccgcca
     3541 gcagtacgac gtcgacggcg cgcgcacgta ctacatcgtc ttcgtcagcg ggcactatgc
     3601 cgacggcaac ggcgtcaacg caggcgtgct cggcgtgtca ttcggcgaca cgatcgcgat
     3661 gttcaaggac gtgattcgaa cgaccgcgtc gctcacgtcg ccgaacagcg agcgctacgt
     3721 cgagcagtcg acgctcatcc acgagctcgc gcactcgatc ggtctcgtcg acaacggcgt
     3781 gccgatggtc gcgggacaca aagacgcgac ccacggcgcg cactgcaaca acgatcaatg
     3841 cgtcatgtac tggctcaatg aaggcgcagc cgacgcgacg tcgttcgccg tcaatcgcct
     3901 gatcaccggc agcacgatcc tgttcgacgc tgcatgcctg tccgacgtcg acgcgaagag
     3961 cggcgggctc tagtagagaa ttgttccttg gtggcccgcg atgaaggcct ggccgacgct
     4021 tgccgaccac acgtcgtaga ggatcgcact cgacggtgtc ggcgcgatcg accaggtcgt
     4081 accgtcgaag cgcgtgactt ttccgagccc gccgacgacc agcacatcac cggcgaggcc
     4141 gccgatatcg tgcgcatcgg gccccatctc gtcgctgttc ggcatcggcg ttgtcgtact
     4201 tccgttgaac aagcccgccc cgaggttgtc gtacgtgtag atctccgtcg acgaaacacc
     4261 cgtgagtctg atgccgctga gcccgccggt gctcgcccac gtgctgccgg tgcggatcca
     4321 gcccgcgcca gaagcggtcg tgacgtagag cttcgtgcca tcgctccaca gcttcgtcgc
     4381 ttcgcgtgcg ctgctgatcg caagcacccc gctgtattgc ggcgtgacgt gagcgaacac
     4441 ggcctgcgtc gcgagcgtcg cgttgccatt tccgacgagc cagacgcccg acgctggcga
     4501 agcccatgcg tccctccaca cggtgtccgt cgtgacgtgc agcgtcgacc aactcgtgcc
     4561 gtcgaagcga tcgatgcgct cggaacctgc ggcgatgacg ttcgtcggtc cggatccggc
     4621 gacggcgtaa tagtcgatcg gaggttgcgg tgccgccatc gcggtgacgt gcgaaccgtc
     4681 gaagtggagg atcgtcccgt tgtcaccgac gaagaagacg tttgcgggcg atgctcccca
     4741 aactgctcgc agcggcatgt acgtgcccga cggcaagcgc gtccagcgac ctgcgcaatc
     4801 agcacggcag ccatcgcccg gaagcttgtt gccgtcgtcg cattcttcgc tcggatcgat
     4861 gatgccgtcg ccgcagccgg cgaatcgaca gccgagacgg cacggcgcac cgggtgcgtc
     4921 cgagtttgcg tcgccggcat cgcactgttc gccgatcgcg atgtcgatgg cgccgtcacc
     4981 gcagacctcc gtcgagtgac agtcagcgct gcagccatcg aagctcgcgc ggttgccgtc
     5041 gtcgcaaagc tcgccttgtt ccggctcgac gaggccgttg ccgcaaccaa tcgggtagca
     5101 gaccgcgccg ttgcagatac ccatcgcgac gcccgtgtac gtgcacgcat cgcggtccgc
     5161 gcggccttcg catgcgtctg cctggatcgc gagcacgcag cggtcacccg acggtgcgca
     5221 caccgatgcc tcggagcaga cgaggctgcc gcacgtcacc gacttcgact gcacgcagct
     5281 cgccaggagc agtagcgcga tgacggcgag cctcatcgct ctacgataga tcaatattcg
     5341 tcgtgatcga cgacggtcat gggaccggcg ttcgcggcgg acagcgcagc ttgcgcatcc
     5401 tcggcggtcg tgacggcgac ggtgacggca tcttcgggac ggaggtgacg cttgcacgcg
     5461 gcacgcacgt cggcggcggt cagctgggcg agcgcttcgg gcaacttcgc ggtgtagcca
     5521 tccggcagct cgaacacggc gtcgcgcacc gcgagctgca tgcgttggcg agcggtcgcg
     5581 atgtggaacg gcatcgcgcc gacgaggtag ctgcgcgcga aatcgacttc gtcgtcggtc
     5641 gggccctgcg cggcgtagtc agcgagcaag tcgagcgtga gcgcgacgca cgggccagca
     5701 tgctcgatcg ccgcggcggt ccagatctcg aaccagtgcg gcaggcgaga gcgacgcagc
     5761 gcgcaaccag cgccgtagct ccaaccgcgc ttgacgcgga tctcttgcat gaggcgcgag
     5821 ctgaacatgc cgccgaacac cgcttcggcg accgcgagcg ctgcggtatc aggatggccg
     5881 tagcgcagtg cgaggtgacc gatgcgcagc tgcgcctgcg tgcgctccgg tttgtcgacg
     5941 acgatgaggc ggcggccgcg acgcgactcg gcttcgtcgc ccgtgtcctc gacgtgcgtc
     6001 gtcgcgcgac caccgggagg gatgcgctcg gtgagccgag agacgatctt cgtcgctgca
     6061 cgctcgtcga tatctccggc gagcccgatg acgagattgt tcgcgaccac ctcgcgcttc
     6121 catagatcgg cggcgatgcc gcgttcgatc tgctcgagcg atgtctcggt gccgagcgac
     6181 gtgcggccat atggatggcc cgggcaacac agccagtcga accagcgcgt agcgagtgcg
     6241 ctgtcgtcat cgcggatctc gtcgaggact tgcggcgtct cgcgcaggag acgcgcgtgc
     6301 tcgtcggtcg cgaacttcgg ctcgccgagg atgtcggcgc acagatcgat cacggcgtcg
     6361 aggtgacgcg acagcgcgag gcccgagagc gtgaccgcat cgcggctgac accgatatcg
     6421 atcatcgcgc cgagcgaatc gagcgtctcg tcgagctgtt ggcgatcgcg cgaaccggca
     6481 ccgcgtcgag cgagcagggc ggcgtggcga tgcaggcctt cgataccgcg cggatcggca
     6541 gcggcgccgc cgcgaatcgc gacgtcgaac cagacgagcg gcgtgtcggg ggaggcctcg
     6601 ataattacgc gtgggcccgc catcaggatt cgccaccgtc gtcttcgtgc tcggcgtcgt
     6661 tgtcctcgcg ctcctcttcc gattccggct cggcgattac gatcgtccgg cgcgtgggca
     6721 agagataggc gcggactaca cgcgcgacgt cgttcgtggt gacttgcgtg agccgctcgg
     6781 caagcacgtt gacgcggcgg aaatcgccga gcgcggtctc gtagtgaccg agcgcttccg
     6841 ctttgccgtc gacgtcgacg agcgagctcc agaaatctgt ctcggcgagg gccttggcct
     6901 tctcgacttc ggcggcagac ggcggcgtct cgatgagcgc gttcagctcc gcatcgatgg
     6961 tcgcgatgac ttcgtcagcg tcatggccgc gcgctgtcgt gaccgcgagg cgaagcagcg
     7021 acggatcgcg aaacggtgtt agctgcgcat cgaccgagga ggccgactcg tcctggatca
     7081 cgagcttgcg atgcagacgc gccgagggac agcccgcgag cagcgtcgac acgatttcga
     7141 gcggcgccca gtctggatcg tcttgtcccg gcgccttgta accaatgagc aggcgatcgg
     7201 tcgcgatcgg tttcggcgcg cgcacgaccc gctcgcgcgt ttgctcgggc tcgacgatct
     7261 tggacggctc cggcagcgtc gccggcggaa tcgcgccgta gtacttcgcg atcgtgtcgc
     7321 gcagctgctc gtcgtcgaaa tcgccgacgc acacgatcgt cgcgttgttc ggcgcgtacc
     7381 acgttcgata aaacgcgcgg atgtccggca gcgcgagcgc gcggatgtct tccatccagc
     7441 cgatcgtcgg ccagcgatac ggatgcgcgg tgaacgcgtg cgccatcagc tgctcgtcga
     7501 gccagccgtc gacgtcatcc tcgacgcgct cgcggcgctc gttcgtgacg acatcgcgct
     7561 cggcttcgac cggcgtcggc tcgagcacga ggtgttgcat gcgctctgct tcgagcttga
     7621 tgccgagctc gagatcgcgc gcgggaagcg agaggcggta gtacgtccag tcgacccacg
     7681 tcgcggcatt gctctcaccg ccggtgcgct cgacttggcg atcgaattcg ccgggcggca
     7741 gcttctcggt ctggccgaac atcaagtgct cgaacagatg cgccataccg gttgcacctg
     7801 gacgctcgtg ccgagagccg acgcgaaacc atgtctgcaa cgcgacgatc ggtgcgcggc
     7861 gatcgatgca cgagatcagc ccgaggccgt tgctgaggcg atagcgacga gctgtcactc
     7921 cgcggcccgc aggtgcctcc gcctcgagct gccacgaaga ctgcgtcatc ggatcgttct
     7981 tagcatgctt acccgaagct gggctcgacg gagagaccga gcgaatcggc gacggcacgc
     8041 agcgcgagcg gtgctcgaaa aagctcgctc ggtgctcgca tcccgaccgg tcctgtggtt
     8101 cgcgtcgcga gctgtcgtgc aacccacgca gcgatgccgg cgctcgcgcg atattgatcg
     8161 cttccacgca cgacgatctg cgcagcatcg aatctg
//
LOCUS       DATLBZ010000530        21565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1435737, whole genome shotgun sequence.
ACCESSION   DATLBZ010000530 DATLBZ010000000
VERSION     DATLBZ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21565
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435737"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VMZ53_26580"
     CDS             complement(<1..420)
                     /locus_tag="VMZ53_26580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS32105.1"
                     /translation="MISVRKLSKRHGARLVLSDVSANVGKGETIAIVGPSGGGKSTLL
                     RCLNYLETFDGGEVDIAGVSLRPGMGGSHREVLRSLRTKVGMVFQQFNLFPHLSALEN
                     ITLAPRVVRGLAPEAAGKRARDLLARVGLGDRGGAYPH"
     gene            complement(395..1867)
                     /locus_tag="VMZ53_26585"
     CDS             complement(395..1867)
                     /locus_tag="VMZ53_26585"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HUS32106.1"
                     /translation="MRHLVVALLVLVAACGDSKHGGTLSAIKKRGEITWGADLQGGEP
                     YLYEDPDHPNHIVGFEVDIANELAKRLGVKATFVQYNWSNLVPSLERGDFDIAMNGLE
                     ATGERKERILMSEPYYVYAETLSVRKGSPYKTLADLKGKRVGTLNQTYAHDLLRAQPV
                     EIVLYEGNEEPYIDLEQGRVDAVLLDNIIADRYGCVPTRPTVVCVPDDIARGTYIIGI
                     RKSDPELKRAIDSALQAMRADGTLQRILVDAKLWDHRQTEAPPEVVDAASSVRQREFD
                     SSMLWQFVLAALVTLKISLLAFLLAVPLGILLAVARVYGGPVFGVFARIYIELFRGTP
                     VLLQLFVLYYGLAPYYSLGPVQAAVLGLGLNYAAYEAEVYRGALLAIPRGQTEAAKAL
                     GMGPTQILRHVLLPQALRLALPPMTNDFVSLLKDSSLVSVITVIELTKRMTIAAVDMR
                     GWLIPGVACAAFYLALSFPLSELARRLERRLARDQRPQAL"
     gene            complement(1889..3397)
                     /locus_tag="VMZ53_26590"
     CDS             complement(1889..3397)
                     /locus_tag="VMZ53_26590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32107.1"
                     /translation="MSIRAATETTNLGRYHVVKRLAKGGMADVLLARTIGVAGFERHV
                     VIKRIHPELGDEPRYRKMFLDEARLAASLHHHNIVQVNDIGEQAGNYFFAMEYVHGED
                     ARTLLIEATNRSQLIPLEHVITIVMAAASGLHHAHEQKGPDKKPLHIVHRDVSPANIL
                     VGYDGGVKVADFGIAFAANRAEQTQSGVLKGKASYMAPEQCNCEPVDRRADVFSLGIV
                     LYELATVNPCFRGENDFMTMSAIVNGKFQRPSLLNAAIPAQLEAIILKALSTRPADRY
                     ATCDEMRIALEQFAEAQNLRTSTTALADDMKQQFGTRPEPWLEEARPDEAEIEITGSS
                     PLDLVLELKLPEPEPEDDLAVPIETTVSKDGMEPLRPVKMTLPLRALKIVPVRAATPP
                     VVPAVEFPEDEEPEVERAVVAPVASQRTRRLWIVASAGLLVVALLLFILVARSSSTTV
                     ANAPAVSPREEIEPRVMMKQHAPAPAAAAPAVTPKPKKAPANKWDPDALFPN"
     gene            3527..4489
                     /locus_tag="VMZ53_26595"
     CDS             3527..4489
                     /locus_tag="VMZ53_26595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32108.1"
                     /translation="MDLARMLDKCVRDQWSIDDLDWSVKPPDLPRDKEEAVVQCFHDM
                     SGIELLAGALFDVQRQKTTDPTLMKIFSTFVADEKRHSAVALRLAKHYDVHHYRDYKE
                     SPSLTDFRPHFLALVRDTSPEIANAYITSGELILDVALLRSLDDYVDDDMSHQAMHLI
                     NRDESRHIAIDFFMTEHYASDEYLAEIARRKRPSLRELVTGIRALATMMWHAKPFLQQ
                     VFLAPMDRTDPSGRRVQEAFKRIQLIMRKPTVARLPFSRFMIGMQDLYNHPIGGRLFG
                     PLLLRALGAEDRAARYLFTKDELTRAQQMSFDEMAEDALAAKFS"
     gene            4569..5810
                     /locus_tag="VMZ53_26600"
     CDS             4569..5810
                     /locus_tag="VMZ53_26600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS32109.1"
                     /translation="MSRTRVEAQLALWSGLTFGSLLTSFMTTRPVRDALVLDGDPSKI
                     PWLFTATFLIFVFIAPIWGRVVQKSPRRVVPASFHVFAAGSLVFALLVEFKVGARYTP
                     YTFYIWAAVYNQFVVSVFWSLLADLVGPTTARTLYGPISAGGTIGAFCGPLLTKYLLD
                     VIGFLGVMTLPVLFLELAVVGAWQIQRIARGLDVTAIEAAKPQQDEPLRENAWAGITR
                     MARSPYLSALGTFTCLTAIAATVVYIAQAKLVQIELPDRHARTGYFATLDFWQQSATF
                     VVQLLVAPVLLRRFGPAPVLCLLPLLQFAGVVGFWLAPSLPLIAAIHISSRTVSLGLT
                     RPARELCFTVLPRDDKYRAKNVIDTLMYRFGDFLSSWMSLALGFSAALVALSVPLTIG
                     WVTLAVVIGLGFRRRMKESTV"
     gene            5807..6946
                     /locus_tag="VMZ53_26605"
     CDS             5807..6946
                     /locus_tag="VMZ53_26605"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUS32110.1"
                     /translation="MKIDRRQFVVGSLAAAAAAACGSGKGPEPVKEPEKKPEPPAKKT
                     ILVLGGTNFIGPHIVTTALARGHTVTLFNRGKTHTELFPEVEKLRGDRDGQLDSLKGR
                     TWDAVVDTSGFVPRIVKMSAELLAPSVKQYVFVSTISVYKQPGTPNMDENAPLETTPE
                     PQSEDIPKYYGALKALSEQAAEAAMPGKVTVVRPGLIIGPGDPTGRFTHWPTRCADGG
                     EVLAPGDGSTPVQYIDGRDLGDWIVHLIEQGTFGTMNALGPDKKITMREVLDACNAAG
                     GNKAKLTWVDWKFLEKQDVAPWAEMPMWIPTEEDPGFGSMSNMRAVKAGLKFRPMAEI
                     AKDTLAWLAALPADTPADKRARMISTGIPRDKETKVLAAWHAAGH"
     gene            complement(7179..7829)
                     /locus_tag="VMZ53_26610"
     CDS             complement(7179..7829)
                     /locus_tag="VMZ53_26610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32111.1"
                     /translation="MTKRFAAVLFVSLIAACVGTPPDDGGPSTGGGNGTGGGNGDGTG
                     GGSGSGDGSGGGGGNGSMTATQFLSQMATKFCDEAFMCKSSFPTDAGVTFAQAFGASA
                     AACVADAAMYDQPALVEQQITAGKIKFSGSDAAACIAGLTFPACTQFWQNGPNSPAAC
                     DTALVGQVADGAACVVDYECSNAQSYCDETSKKCTPDTSGARTTPRLGEPDFGTMY"
     gene            complement(8017..8547)
                     /locus_tag="VMZ53_26615"
     CDS             complement(8017..8547)
                     /locus_tag="VMZ53_26615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32112.1"
                     /translation="MRVLGVLLVCLTTACVADVNGTHQESKEEETATTGGGAESGNTT
                     TTPTGYLSQIADIYCSESFTCRADFPPDRGYTFEAQWGASENECVNRLLTGWNPAAIE
                     NEIAKGRAMYDGAAGIACLNGVTFGSCSDYWTSGIQWAEACYHVIIGLVPAGGACENM
                     YACASQSCNATTHTCL"
     gene            8685..11042
                     /locus_tag="VMZ53_26620"
     CDS             8685..11042
                     /locus_tag="VMZ53_26620"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32113.1"
                     /translation="MGAVSIGAAVLAAILLLCTYITHPIPRFIGKPLGVHQGDFADRG
                     GLVMTWEAPTVDEDRLRPIEEAMRARNPDGYVWREGRLLKVALAGVPPEQVDAVVARM
                     TKGEGLTFREVLIVPEMKQIGELVPKENFEIDQWRPEDGGPIHTDYYLFAPTLEELDA
                     LREDVAAKGVNLPEGAVIAYEKIDSYDGKRGYRTYVVREQPALDGTAIADATLSYDPN
                     TNRPIVLLDFTRDGGRIFGELTSRIVGEKLAALIDGEVRSAPVINGPIRGGRASITMG
                     GADPQQQENEARALVETLKFGNLPAGGHVVEARYQPPTKSPSRLWLARVLLALLAAFV
                     VGGLAYVTLRFARPERQAFAPKRAGAAPWSRLAVMLVAPVAVIGVAQVTMFGVNDVEL
                     THIMARGGRRDFDFVTIGALGIMPVLTAYVVVEAFSLILPTWRRRRNAGPIARQPITL
                     AAAIVSVLLIVFQGWNMAMYMSRIEDVLYPGFFGKLLVVTSVAAGTLILIVAAEVIRW
                     RGLGNGYGALLATGFIGSLIPDDFGPQIAHDDRIVQLLGIAIIAIVFFVGLRWRVSQR
                     GEHSIRFPSSSFAPLAYAGGAVALIGLASQFPVEEFLFKALTWMQRVDSSAMAQLSLV
                     AALAIIWSFAFGRPGPFGGEARPDVITWLRATSLSLVALVLVAAVSVLADSVTEPPPF
                     VSVRTLLVDPIMIAITVAVVMDAIADFRARREKLVVAWTLHEPHHVERVQRLLAANDI
                     QPHMSALHLRSLLSFFGPFAPIEVQVPLAQGEQTREILERLAAEA"
     gene            complement(11039..11788)
                     /locus_tag="VMZ53_26625"
     CDS             complement(11039..11788)
                     /locus_tag="VMZ53_26625"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HUS32114.1"
                     /translation="MCGRYTITRQDSLVHDIEAAMGQLQLDPAVASNQWWKPRFNVAP
                     TQDAPVITLRDGVKTLEMMRWGLVPFWAAKEGAAKPPLMINARVEGIDSKRVFRDALE
                     RKRCLVPADGFFEWKREGKGKTAKKTPMWIHPGDHKFFMLAGLWSRAKLPAGELHSFT
                     IITGPPNELVSPIHDRMPVVLARETWQAWLDPSLPLEGAHALLGVPPIDDWKAEPVSS
                     WVNVADHDDPKCVEPAPPDNSSVPAQGKLWD"
     gene            complement(11793..12974)
                     /locus_tag="VMZ53_26630"
     CDS             complement(11793..12974)
                     /locus_tag="VMZ53_26630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS32115.1"
                     /translation="MKLATLSSAAANFIANGGRDLDIARAGELPALAAGDHVKLVDAN
                     GDDVGLAIADPDNGKLRVLALPTDGFAAIDGALIGWRVEKAVAWRKQLGLPGDGHAYR
                     VVHGAGDDLPGFAADVLGKYACVYVYGEGLRPLARQLADAIVGFARLDGAVVKLRARG
                     SADDVKQETVGKAPPETFVVAEPGVPELQYEIHPHGGLNVGLFTDMREQRRGLHRFVG
                     GKRVLNLFSYTGALSLASAKAGAASVTSVDTSQGVNAWAMGNFTRSGFQPGAQYKFET
                     GDAVRFLARAIRDKERYDVVLIDPPTFSTARGSPWTLDRDYPALIAQAAEVIPAGGVL
                     WLAANTHELGSLAKLAQKGIRNAGRNAAIIEQGGLPPEYPTVAAQPHDRYLQICVLRL
                     T"
     gene            complement(12974..13864)
                     /locus_tag="VMZ53_26635"
     CDS             complement(12974..13864)
                     /locus_tag="VMZ53_26635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32116.1"
                     /translation="MRTQAVLAAFLAVAACGPGPERGDDDDGTGSGSGSGSGSGSGNA
                     DGCSDAAKLVYVVDSNNKFSKFDPMTKTFMDIGTLSCPGENGGLFAGQPFSMGIDRDA
                     VAWVLFSDGKLFRVDTSNAACTSTTWTTQQGEMLFGMGFSANTAGSTDDTLFIAGGAG
                     DPMSSPTSKLATLSTTSFTAAPLGTVQGWPELTGNGNAELWGWFPDASAPRIEQINKT
                     TGAPIKTYNLPSLAGTPAAWAFAFWGGDYWIFLEKSNETKTTVYQVKGDTGVITTMTP
                     ATGRTIVGAGVSTCAPVVIL"
     gene            13915..19107
                     /locus_tag="VMZ53_26640"
     CDS             13915..19107
                     /locus_tag="VMZ53_26640"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32117.1"
                     /translation="MGQPEEPSTELLPAAGEAQAQGIAHPQVTPEGQPEPTLIGRTLG
                     RFTVVEKLGRGGSGEVFRAEQQQLGRSAVIKVLRREVAMAPNRVERFLREAKLASRLD
                     HPYAAHIYAFGAETDNVLWIAMEHVKGVTLDDLVARRGAMPAQVFAPLFVRLCEVIHT
                     AHELDIVHRDIKGANVMVIERAGQLLPKLLDFGIAKGDAFATTPGVVDGELTGHGVTL
                     GSPHYMSPEQWERPADVDARSDIYALGVLAYRCLAGFLPFHTTSRLQLAHAHTHLPVP
                     AMPDFVSPSLADVIMKALAKQPEQRWQTAVAFAEAVQRAVGTPVTEAVPLFDPATRDA
                     WLSAGPQPIADAVAHVTSATTTVEVDAALRELVAITCRWLAVLSLSGLPPATGTDPSV
                     REHARAVVGRDDGAPWLRLAKAAVAASPTPLMALVAALEASEPLAKLADRLDDPHRQR
                     TAALLAADVGAAAEALSALEPLLAYHLVLGGAGAVAESWQGPRRRDREKVLVWGELAE
                     SEVALLDTAGKVVARLSPYAQVIAPLPAAEPELFLLWRSGRGQARLVAAPWGFERDDE
                     RAGTMLAALSTEDSDTAMDAQDERSPYPGLAAYGTADADHFVGREREVETLANRLVRA
                     PLIAVLGPSGVGKSSFIHAGLVPRLGEQHRVLTMRPGRHPLHALAALPPVSGDSHDEG
                     ALVARLRELGESAQRGLVLVVDQLEEMVTLCADPDERRRFAETLAGAADGPNAPVRVV
                     ATLRDDFATVIESEAAFRGQFDVFVLATPPPESLRRIVVEPARRSAVSVDTRVVDDMV
                     AEVAGRPASLPLLSFTASQLWQTRDRQARKITYDAYLELGGVAGALATYADQVYGSLA
                     RRDQETVRDVFARLVAANGTRVPSPRSDLEQLPGAKGVLGHLIDARLLVVRDVEGQDI
                     VEIVHECLAERWPRLARWRSEDAADRALLADVTTASRRWIEAGRRADLLWRGEALAEL
                     SRLTARSTAMTGDERAFADAAVLAQTRARRIRRTLIAASMLVLAGVAIVMAYLSVVAN
                     ENRAEAERSAKKAADAASLAEQRLTQSLIAQGRRELNDGRDMPALAYFAAALERGADT
                     PALREMVSLAERGWPYFLTTHRGTAGGRAIVTMAGSPDGWIVGGDDSGSLRWWNDEGA
                     LIGELKTELQSITGIDRNPDNSLVVSGLRGIVIVDASHRIVQKLAIADEPWFARRGPG
                     PDEVSALASDALRVYGLDGVERRSMPITRTGDTQEPMLDEGARHAIFAADGELSVVDL
                     KTMKIRSLAHGIHNEPNGANDGSRYAYIDHDWNVHLLRGDGTEIKKIKPDTRPIRLVL
                     SDDGTRLGITSEQRFDVFDEKGELLDGIPLKLEQASYAMRGDDVWVTNQEGTVRHYVE
                     GQLVASAPLHASEIRLVLVARNALATVGSDSTLVMMKADARQVVEMPHLCKHISFAQY
                     GVATGYTCGDTTLLYTGRKKLGEYPETMMMVQVMHEPAYDRTALAADRGIRVFDGDAK
                     EIARTDKFLGAAWADRDHLWAVTDRKTLSRWAFATDTWEKIGEVPESSAVGSIPGYLL
                     IGTEDGEVHVLVDGREQHVVAIGTPVVGFNASPDHRWVAAQLSSGAAAIISTTTWTLE
                     RTLAQGDANGDFPIFDATGDLVLRANHYALTIWDRATGEELVFGLDLLSDLGGGRWLP
                     DGRIEMNRRRPSLLDIPRDKRPVPDILADIACHVPLKVVGSRIEPTVPIACAAGVSGL
                     TLPK"
     gene            19104..20432
                     /locus_tag="VMZ53_26645"
     CDS             19104..20432
                     /locus_tag="VMZ53_26645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS32118.1"
                     /translation="MIDDDAGSTTEQIDRQTPFGDRVLLVIDGASLQVVPLPPTGTLT
                     VGRSQKCDISIDSGSVSRHHANVVVGNDVEVEDVGSSNGTFVDGVRLPGNKKVRVGVG
                     IPFLIGGVTVMVQTRAGQRRDSASKSSALAALEQSASRIAVGKLSVLILGETGVGKER
                     FAERLHEMSPRRSNAFVRINCAAISEPLLEAELFGNEATNKPGLIEYADGGTAFLNDV
                     AELPQSLQVKLLRVIEDNAVRRIGSPNPRNVDVRFLAATNKDLAAEVDAGRFRRDLYF
                     RLAGASFTIPPLRDRKDEVLPLAEQFVASASGPLGRHFVLADDAKQWLAAHDWPGNIR
                     ELRNACERAVLLATGSMIEKQHLTIDEPKRPPGRFRSPTTPPPGMITSDMPTQVRATV
                     AELEKQRILEALDKCAGNQTRAAELLGISRRTLINRLDEYGIARPRKRDE"
     gene            complement(20449..>21565)
                     /locus_tag="VMZ53_26650"
     CDS             complement(20449..>21565)
                     /locus_tag="VMZ53_26650"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS32119.1"
                     /translation="PQGSAKIISGHLQLIFQYPNLSPYVVPFVTIGDGFMHTSSDVLG
                     SDTDWPLNTDIGARFLVHRSITLRAEGRFLRGASAKAPYTLDASYAEFMFGISFRPSK
                     APPEPPPPPPPPKVDSDGDGMFDDVDKCPNQAEDIDLFDDTDGCPDPDNDADGVFDTT
                     DKCPLEPEDKDGNADEDGCPDPDDDADGVPDKQDKCLREAEDKDGFQDLDGCPELDND
                     KDSFLDAQDKCPLEAEVINGTEDEDGCPDRGNALVVISPDRLELLENVVFKKTIIQKD
                     SFNLLNQVGAQLRAHPEILRLRITMHVQPTKNAEADQRLSELRAFAIREYLIKYGIDE
                     KRLEPRGFGGTKPLVDPKSKGAKDINERLELIILERK"
BASE COUNT         3524 a         7030 c         7219 g         3792 t
ORIGIN      
        1 gtgcggatat gcgccaccgc gatcgccgag gccgactcgc gcgagcaaat cacgcgcgcg
       61 cttgcccgct gcctccggcg cgagacctcg gactacgcgc ggcgccagcg tgatgttctc
      121 gagcgcggag aggtgcggga acaggttgaa ctgctggaac accatgccga ccttcgtccg
      181 cagcgagcgc aacacctcgc gatgcgagcc acccatgccg ggccgcaggg agacgccggc
      241 gatgtcgacc tcgccaccgt cgaacgtctc gagatagttc aggcagcgca gcagcgtcga
      301 cttgcctccg ccggaggggc ccacgattgc gatcgtctcg cccttgccta cgttcgcgga
      361 gacgtcgctg agcacgagcc gtgcgccgtg tcgcttagag agcttgcgga cgctgatcac
      421 gcgcgagcct cctttcgaga cgacgagcga gctcggacag cgggaacgag agcgcgagat
      481 agaacgccgc gcacgcgacg cccgggatga gccagccacg catgtcgaca gcggcgatcg
      541 tcatccgctt cgtcagctcg atcaccgtga tgacactcac cagcgagcta tccttgagca
      601 gcgatacgaa gtcattcgtc atcggcggca gcgcgaggcg cagcgcctgc ggtagcagca
      661 cgtgtcgcaa gatctgcgtc ggtcccatgc cgagcgcctt ggccgcctcg gtctgcccac
      721 gagggatcgc gagcagcgca ccgcgataga cctcggcttc ataggccgcg tagttcaagc
      781 ccagtcccag caccgccgct tgcactgggc cgagcgagta gtacggcgcg aggccgtagt
      841 agaggacgaa cagttgcagc agcaccggcg tgccgcgaaa cagctcgatg tagatccgcg
      901 cgaagacgcc gaagacggga ccgccgtata cacgcgcgac tgccagcagg atcccgagcg
      961 gcacggcgag caggaacgcc aagagggaga tcttcagcgt cacgagcgct gcgagcacga
     1021 actgccagag catcgacgag tcgaactcgc gctggcgcac cgacgatgca gcatcgacga
     1081 cctcgggcgg cgcttccgtc tggcggtgat cccagagctt cgcgtcgacg aggattcgct
     1141 gcagcgtgcc gtccgcgcgc atcgcttgga gcgcgctgtc gatcgctcgc ttcagctccg
     1201 ggtcggactt gcgaatgccg ataatgtacg tgccgcgcgc gatgtcgtcc ggaacgcaga
     1261 caacggtcgg ccgcgtgggc acgcatccgt atcggtccgc gatgatattg tcgagcagca
     1321 cggcatcgac gcgcccctgc tcgagatcga tgtacggctc ctcgttgcct tcgtagagca
     1381 cgatctcgac gggctgcgcg cgcagcaggt catgcgcgta cgtctggttg agcgtgccga
     1441 cgcgcttgcc tttgagatcg gcgagcgtct tgtatgggct gccctttcgc acggacagcg
     1501 tctccgcgta gacgtagtac ggctcgctca tcaggatgcg ctctttgcgc tcgcctgtgg
     1561 cttcgaggcc gttcatcgcg atgtcgaagt cgccgcgctc gagcgatggg acaagattcg
     1621 accagttgta ttggacgaac gtcgccttca cgccgaggcg ttttgcgagc tcgttcgcga
     1681 tgtcgacttc aaagccgacg atgtgattcg ggtggtccgg gtcttcgtag agatacggct
     1741 cgccgccctg caggtctgcg ccccacgtga tctcaccgcg cttcttgatc gcggacagcg
     1801 tgccgccgtg cttgctatcg ccgcacgctg cgacgagcac gagcagagca acgacgaggt
     1861 ggcgcaccgg cgccagtata gacgtctgct aattcgggaa cagcgcgtcg ggatcccact
     1921 tgttcgcggg agccttcttc ggcttcggcg tgacggccgg cgcggcggcg gcgggcgcgg
     1981 gcgcatgctg cttcatcatc acgcgcggct cgatctcttc gcgcggcgac acagcggggg
     2041 cattcgcgac ggtcgtgctc gacgagcgag cgacgagaat gaacaacaac agcgcgacga
     2101 cgagcaggcc cgccgaggcg acgatccaga gccgcctcgt ccgttgcgat gcgaccggcg
     2161 ccacgacggc tcgctcgacc tcgggctctt cgtcctcggg aaactccacc gcgggaacca
     2221 ccggcggcgt tgccgcgcgc accggcacga tcttcagagc gcgaagcggc agcgtcatct
     2281 tcaccggccg tagcggctcc atcccgtcct tcgagacggt ggtctcgatc ggcacggcga
     2341 ggtcgtcctc gggctccggc tcgggcagct tcagctccag cacgagatcg agcggcgagc
     2401 tgccggtgat ctcgatctcg gcctcgtcgg gccttgcctc ttcgagccac ggctcggggc
     2461 gcgtgccgaa ctgctgcttc atgtcgtcgg cgagcgcggt cgtcgacgtc cttagatttt
     2521 gcgcctcggc aaactgctcg agtgcgatgc gcatctcgtc gcacgtggcg tagcgatcgg
     2581 cgggccgcgt tgacagcgcc ttgagaatga tcgcttcgag ctgcgcgggg atcgccgcgt
     2641 tgagcagcga cgggcgctgg aacttgccgt tgacgatcgc cgacatcgtc atgaagtcgt
     2701 tctcgccgcg gaagcacggg ttcacggtcg ccagctcgta gaggacgatg ccgagcgaga
     2761 agacgtcggc gcggcgatcg accggctcgc agttgcattg ttcgggcgcc atgtacgacg
     2821 ccttgccctt cagcacgccc gattgcgttt gctctgcgcg attcgcggcg aacgcgatgc
     2881 cgaagtcggc gactttcacg ccaccgtcgt agccgaccag gatgtttgcg ggcgagacgt
     2941 cgcgatgcac gatgtgcagc ggcttcttgt cgggaccctt ctgctcgtgc gcgtggtgca
     3001 agcccgacgc ggcggccatc acgatcgtga tgacgtgctc gagtgggatc agctggctgc
     3061 gattcgtcgc ttcgatcagc agcgtgcgcg cgtcttcacc gtgcacgtac tccatcgcga
     3121 agaagtagtt gccggcctgc tcgccgatgt cgttgacctg gacgatgttg tggtgatgca
     3181 gcgatgcggc gaggcgcgct tcgtcgagga acatcttgcg atagcgaggc tcgtcgccga
     3241 gctcgggatg aatccgcttg atgacaacgt gacgctcgaa gccggcgaca ccaatcgtgc
     3301 gcgcgagcag gacgtcggcc atgccgccct tcgcgaggcg cttgaccacg tgatagcgac
     3361 cgaggtttgt cgtttcggtc gcggcgcgaa tgctcacaga accaaaatga ggcatcgcgg
     3421 atgtatgggt caagtgctct aaaccattgc cgaatgtagg cgctgtcggt gcgatctttt
     3481 tcgtatcgcg tatgacgatc cttgctcgcc gagtaccatt ggccgcatgg acctcgcgcg
     3541 catgctggac aagtgcgtcc gcgaccagtg gtcgatcgac gacctcgact ggagcgtgaa
     3601 accacctgat cttccacgcg acaaggaaga agcagtcgtc cagtgcttcc acgatatgtc
     3661 gggcatcgag ctgctcgcgg gtgcgctgtt cgacgtgcag cgtcagaaga cgaccgaccc
     3721 gacgctcatg aagattttct cgacgttcgt cgcggatgag aagcggcact cggccgtcgc
     3781 actccgtctc gcgaagcact acgacgttca tcactaccgc gactacaagg agagcccgtc
     3841 gctcaccgac ttccgcccgc acttcctcgc gctcgtgcgc gacacgtcgc cggagatcgc
     3901 gaacgcgtac atcacgagcg gcgagctcat cctcgatgtc gcgctgctgc gctcgctgga
     3961 cgactacgtc gacgacgaca tgagccatca ggcgatgcac ctcatcaatc gcgacgagtc
     4021 acgtcacatc gcgatcgact tcttcatgac ggagcactac gcgagcgacg agtacctcgc
     4081 ggagatcgcg cgtcgcaaac gcccttcgct gcgcgagctg gtcaccggca tccgcgcgct
     4141 cgcgacgatg atgtggcacg ccaagccgtt cttgcagcag gtcttcctcg cgccgatgga
     4201 tcgcacagac ccgagcgggc gccgcgttca ggaagcgttc aagcggatcc agctcatcat
     4261 gcgcaagccg acggtcgcga ggctcccgtt ctcgcgcttc atgatcggaa tgcaggatct
     4321 ctacaaccat ccgatcggcg gccggctgtt cggcccgctg ctgctgcgtg cactcggtgc
     4381 cgaggatcgc gcggctcgct acctgttcac gaaggacgag ctgacgcgcg cccagcagat
     4441 gagcttcgac gagatggcgg aagacgcgct cgctgcgaag ttctcctgag ccgagaccga
     4501 gtcgcacgct gtcgcacgcg tcgcagactt gacggcatga ccgccgtccg ccgacggtag
     4561 gcgcgcacgt gtcgcgtact cgcgtagaag ctcagctagc gctctggagc gggctcacgt
     4621 ttggctcgct gctgacgagc ttcatgacga cgcggccggt ccgcgatgcg ctcgtgctcg
     4681 acggtgatcc gtcgaagatc ccgtggctgt tcaccgcgac gtttctgatc ttcgtgttca
     4741 tcgcgccgat ctggggacgc gtcgtgcaga agtcgccgcg ccgtgtggtg ccggcgtcgt
     4801 ttcacgtctt cgcggcgggc tcgctcgtgt ttgcgctgct cgtcgaattc aaggtcggcg
     4861 cgcgctacac gccgtacacg ttctacatct gggctgcggt ctacaaccag ttcgtcgtca
     4921 gcgtgttctg gagcttgctc gcggatctcg tcggaccgac gacggcgcgc acgctctacg
     4981 gcccgatctc ggcgggcggc acgatcggcg cgttctgcgg gccgctcctg acgaagtacc
     5041 tgctcgacgt gatcggcttc ctcggcgtga tgacgctgcc cgtgttgttc ctcgagctcg
     5101 cggtcgtcgg cgcctggcag atccagcgca tcgcgcgcgg tctggacgtc accgcgatcg
     5161 aagcggcgaa gccgcagcaa gacgagccgc ttcgcgagaa cgcgtgggcg ggcatcacgc
     5221 gaatggcgcg ctcgccgtat ctctcggcgc tcggcacatt cacatgcctc accgcgatcg
     5281 cagcgacggt cgtctatatc gcgcaggcca agctcgtgca gatcgagctg ccggatcgcc
     5341 acgcgcgaac gggctacttc gcgacgctcg atttctggca gcagagcgcg acgttcgtcg
     5401 ttcagctgct cgtcgcgccg gtcctgctgc gccggttcgg gccggcaccg gtgctgtgcc
     5461 tgctgccgct tttgcagttc gcgggtgtcg tcgggttctg gctcgcgcca tcactcccgc
     5521 tcattgcggc aattcacatt agcagccgca ccgtatcgct cgggctcacc cgcccagcgc
     5581 gcgagctgtg cttcaccgtg cttccccgcg acgacaaata tcgagccaag aacgtgatcg
     5641 acacgctcat gtatcggttt ggcgatttcc tgagctcgtg gatgtcgctc gcgctcgggt
     5701 tttcggccgc gctcgtcgca ctgtctgttc cgctcacgat tggctgggtc acgctcgcgg
     5761 ttgtcatcgg cctcggtttt cgacgccgca tgaaggagtc cactgtatga agatcgatcg
     5821 tcgtcagttc gtcgtcggat cactcgccgc tgctgcggcc gctgcatgcg gaagcgggaa
     5881 aggaccggag ccggtgaaag agcccgagaa gaagccagag ccgcctgcga agaagacgat
     5941 cctcgtgctc ggcggcacga acttcatcgg cccgcacatc gtcaccacgg ctctcgcgcg
     6001 cggccacacc gtcacgctgt tcaatcgcgg caagacccac acggagctgt tccccgaggt
     6061 cgagaagctg cgcggcgatc gcgatggcca gctcgattcg ctcaagggcc gcacctggga
     6121 cgcggtcgtc gatacgagcg gcttcgtgcc ccgcatcgtg aagatgtcgg cggagctgct
     6181 cgcgccgagc gtgaagcagt acgtgttcgt ctcgacgatc tcggtctaca agcagccggg
     6241 cacgccgaac atggacgaga acgcgccgct cgagacgaca cccgagccgc agagcgagga
     6301 catcccgaag tactacggcg cgctgaaggc gctcagcgag caagcggccg aagcggcgat
     6361 gcctggcaag gtgaccgttg tccgccccgg cctcatcatc ggtcccggcg atccgacggg
     6421 gcggttcacg cattggccga cgcgctgcgc cgatggcggc gaggtgctcg cgccgggcga
     6481 tggctcgacg ccggtgcagt acatcgatgg ccgcgatctg ggcgactgga tcgtccacct
     6541 gatcgagcaa ggcacgttcg ggacgatgaa cgcgctcggc cccgacaaga agatcacgat
     6601 gcgcgaggtg ctcgatgcct gcaacgccgc cggcggcaac aaggcgaagc tcacgtgggt
     6661 cgactggaag ttcctcgaga agcaggacgt cgcgccgtgg gcggagatgc cgatgtggat
     6721 cccgaccgaa gaagatccgg gcttcggctc tatgtcgaat atgcgcgcgg tgaaagcggg
     6781 tctgaagttc cggccgatgg cagagatcgc gaaggacacg ctcgcgtggc tcgcggcgct
     6841 tccggcggat acgcccgccg acaaacgtgc gcgcatgatc tcgacgggca tcccgcgcga
     6901 caaagaaacg aaagtgctcg cggcgtggca cgcagcgggt cactgagcgg ccactcgcgt
     6961 agccgtcacc cgcgatcacc gggttcgtaa accgccggga tcaatcacgg ggctcgcgag
     7021 cgtcgcaacc tcgcgtccct attcgccggc cctcgtggca catcgactgc actaagggat
     7081 tggcgttcga caagaatgga tcggctggag acggctggtt cggggcggct cgtacctggt
     7141 ggatgcgcaa aatctaacga gccgccccac acatttttct agtacatcgt gccgaagtca
     7201 ggctcaccga gcctcggcgt tgtacgagca cccgacgtat ccggggtgca cttcttcgag
     7261 gtctcgtcgc aatacgactg cgcgttgctg cactcgtagt cgacgacgca cgcggcaccg
     7321 tcggcgactt ggccaacaag cgcggtgtca cacgcggcgg gcgaattcgg gccgttctgc
     7381 cagaactgcg tgcatgccgg gaacgtgagg cctgcgatgc acgccgctgc gtcactgccg
     7441 ctgaacttga tcttgccggc ggtgatctgc tgctcgacga gcgcaggctg gtcgtacatc
     7501 gccgcgtccg caacgcacgc agcagccgac gcaccgaacg cttgagcgaa cgtgacgccc
     7561 gcgtcggtcg ggaagctcga cttgcacatg aatgcttcgt cgcagaactt cgtcgccatc
     7621 tgcgagagga actgcgtcgc ggtcatgctg ccgttgccgc caccgccacc actgccatcg
     7681 ccgcttccac tgccaccgcc ggtgccgtcg ccgttaccac cgccggtgcc attgcctcct
     7741 ccggtagacg gaccgccgtc gtcgggtggt gttcccacgc aggctgcgat cagtgaaaca
     7801 aacagaactg ctgcgaaacg tttcgtcatc gggactgctc cttgtcgatg caatccgcag
     7861 tttgcaactg agtggccagc tcccagtgcg acgcgcgatc acacacttcc tcgcgcgaac
     7921 gcgatcagcg ctgctcaggt cgtgcaacgt ggtgcgcgag atcgtcgacg aagccgggga
     7981 acgtgtcgcc ggctacagtc ccggtcacgt tcgcgatcag aggcacgtat gcgtggtcgc
     8041 gttacacgac tgcgaagcgc acgcgtacat gttctcgcaa gcgccgcccg cgggaacgag
     8101 accgatgatc acgtggtagc aggcctcggc ccactgaatg ccggaggtcc agtagtcgct
     8161 gcacgagccg aacgtcacgc cgttcaagca cgcgatgccg gcagcgccgt cgtacatcgc
     8221 gcggcccttc gcgatctcgt tttcgatcgc ggccgggttc cagccggtga gcaggcggtt
     8281 gacgcactcg ttctcgctcg ctccccactg cgcttcgaac gtgtagccac gatcgggcgg
     8341 gaagtcggcg cggcaggtga acgactcgga gcagtagatg tcggcgatct ggctgaggta
     8401 gccggtcggc gtcgtcgtgg tgttgccgga ctcggcgccg ccgccggtcg tcgcggtctc
     8461 ctcttccttg gactcctggt gcgtgccgtt gacgtccgcc acgcaggcgg tggtcaggca
     8521 gacgagcagg actcccagga cgcgcatcaa ggcgaggaaa tgcaatgggt ttgccatcgt
     8581 ggctagtcca gtagccactt cgcaagcctt cgagatcgtg cgaaaaggcg ccgatgactg
     8641 acggaggggc aggcgtttcg accggagatg ggaccccgat ccgggtgggg gcagtctcca
     8701 tcggggcggc agttctcgcc gcgattctcc tgctttgcac ctacatcacg cacccgattc
     8761 cgcgcttcat cggcaagccg ctcggggtgc atcaaggcga cttcgcggac cgcggcggcc
     8821 tggtgatgac gtgggaagcg ccgaccgtgg acgaggatcg gctgcgcccg atcgaagagg
     8881 cgatgcgagc gcggaacccg gacggctatg tctggcgcga aggccgcttg ctgaaggtcg
     8941 cgctcgcagg agtaccgccg gagcaagtcg atgcggtcgt cgcgcgcatg acgaagggcg
     9001 aaggcctgac gtttcgcgag gtgctgatcg ttcccgagat gaagcaaatc ggcgagctcg
     9061 tgccgaagga aaacttcgag atcgatcaat ggcgtcccga ggacggtggc ccgattcaca
     9121 cggactacta cttgttcgcg ccgacgctcg aggagctcga tgcgctgcgt gaagacgtcg
     9181 ctgcaaaggg agtgaacctt cccgagggtg ccgtcatcgc gtacgagaag atcgattcgt
     9241 atgacggcaa acgcggctat cgcacgtacg tcgtgcgcga acagccagcg ctcgatggca
     9301 ccgcgattgc ggatgcaacg ctgtcgtacg atccgaacac gaaccggccg atcgtgctgc
     9361 tggatttcac gcgcgatggc ggccgcatct tcggtgagct gacctcgcgt atcgtcggtg
     9421 aaaagctcgc ggcgctcatc gatggtgagg tgcgatcggc gccggtcatc aacggtccga
     9481 tccgcggagg ccgcgcgtcg atcacgatgg gtggggcgga tccccagcag caggagaacg
     9541 aggctcgagc gcttgtcgag acgctcaagt tcggcaacct gccagccggc gggcacgtcg
     9601 tcgaagcgcg ctatcagccg ccgacgaaga gtccatcgcg cctgtggctc gcgcgcgtgt
     9661 tgctcgcgct gctcgccgcg ttcgtcgtcg gtggtctcgc gtacgtgacg ctgcggttcg
     9721 cgcggccgga gagacaagcg ttcgcaccga agcgcgcggg ggcggctccg tggtcgcggc
     9781 tcgcggtgat gctcgttgcg cctgtcgcgg tcatcggcgt cgcgcaggtg acgatgttcg
     9841 gcgtcaacga tgtcgagctg acgcacatca tggcgcgtgg cggtcgccgt gacttcgact
     9901 tcgtcacgat cggtgcactc ggcatcatgc ccgtgctcac ggcgtacgtc gtcgtcgaag
     9961 cgttctcgct gatcctgccg acgtggcgtc gccgccggaa tgcgggcccg atcgcgcgcc
    10021 agccgatcac gctcgccgcg gcgatcgtca gcgtgctgct gatcgtgttc caaggctgga
    10081 acatggcgat gtacatgagc cgcatcgagg acgtcctcta tccgggattc ttcggcaagc
    10141 tgctcgtggt cacgtcggtc gccgcgggca cgctgatcct catcgtcgcg gcggaggtca
    10201 ttcgctggcg cggcctcggc aacggctacg gtgcgctgct cgcgacgggc ttcatcggct
    10261 cgctgattcc cgacgatttc ggcccgcaga tcgcacacga cgatcgcatc gttcagctgc
    10321 tgggcatcgc gatcatcgcg atcgtgttct tcgtcgggtt gcgctggcgc gtgtcgcaac
    10381 gcggcgagca ctcgattcgc ttcccgagct cgagcttcgc tccgctcgcg tacgccggcg
    10441 gtgcggtcgc gctgatcggg ctcgcgtcac agtttcccgt cgaagagttt ctgttcaagg
    10501 cgctcacctg gatgcagcgc gtggattcgt ccgcgatggc gcagctatcg ctcgtcgccg
    10561 cgttggcgat catctggtcg ttcgcattcg gccggcctgg cccgttcggc ggggaagcgc
    10621 gtcccgacgt gatcacgtgg ctacgggcta cgtcgctgtc gctcgtcgcg ctcgtgctcg
    10681 tcgcggctgt aagcgtgctc gccgatagcg tcaccgagcc gccgccgttc gtcagcgtcc
    10741 gcacgctgct cgtcgatccg atcatgatcg cgatcaccgt cgcggtcgtg atggatgcaa
    10801 tcgccgactt ccgcgcgcgt cgcgagaagc tcgtcgtcgc atggacgctc cacgagccgc
    10861 atcacgtcga gcgcgtgcaa cgcttgctcg ccgcgaacga catccagcct cacatgagcg
    10921 cgttgcacct gcgatcgctg ctgtcgttct tcgggccgtt cgcaccgatc gaagtgcaag
    10981 taccgctggc tcagggcgag cagacccgcg agatcctaga gcgactcgcg gccgaggcct
    11041 aatcccagag cttgccttgc gcggggaccg atgaattgtc gggcggcgcg ggctccacac
    11101 attttggatc gtcgtggtcg gcgacgttca cccacgacga caccggctcg gccttccaat
    11161 cgtcgatggg cggtacgccg agcaacgcat gtgcgccttc gagcggcagc gatggatcga
    11221 gccacgcctg ccacgtctcg cgcgcgagca cgacgggcat tcgatcatgg atcggtgaca
    11281 cgagctcgtt cggcggtccc gtgatgatcg tgaagctgtg cagctctccc gcggggagct
    11341 tcgcgcgcga ccacagaccc gcgagcatga agaacttgtg gtcgccggga tggatccaca
    11401 tcggtgtctt cttcgcggtc ttgcccttgc cctcgcgttt ccactcgaag aagccgtcgg
    11461 caggaacgag gcagcgcttg cgctcgagcg catcacgaaa cacgcgcttg ctatcgatgc
    11521 cctcgacgcg cgcgttgatc atgagcggcg gcttggcggc gccttctttc gcggcccaga
    11581 acggcacgag cccccagcgc atcatctcca gcgttttcac gccgtcgcgc agcgtgatga
    11641 ccggcgcgtc ttgcgtcggc gccacgttga agcgcggctt ccaccactga ttgctcgcca
    11701 ccgcgggatc gagctgcagc tgacccatcg cggcctcgat atcgtggacg aggctgtcct
    11761 ggcgcgtgat cgtgtagcgg ccgcacatgg cgctacgtaa gccgtagcac gcaaatctgc
    11821 aggtagcgat cgtgcggctg tgccgcaacc gtcgggtact cgggcggcaa tccgccttgc
    11881 tcgatgatcg ctgcgttgcg ccccgcgttg cggatgcctt tttgcgcgag cttcgcgagc
    11941 gagccgagct cgtgcgtatt cgccgcgagc cagagcacgc cacctgcggg aatcacttcg
    12001 gcggcttgcg cgatgagcgc cggataatcg cgatcgagcg tccacggcga gccgcgcgca
    12061 gtcgagaacg tcggtggatc gatgagcacg acgtcgtaac gttctttgtc ccgaatcgcg
    12121 cgcgcgagga agcgcacggc atcgccggtc tcgaacttgt actgcgcgcc cggctgaaac
    12181 cccgagcgcg tgaagttgcc catcgcccac gcgttcacgc cttgcgacgt gtcgacgctc
    12241 gtcacgctcg ccgcacccgc ctttgccgat gcgagcgaca gcgcgccggt atacgagaac
    12301 agattcaaca cgcgcttgcc gccgacgaag cggtgaaggc cgcgccgttg ctcgcgcata
    12361 tcggtgaaga ggccgacgtt gaggccgccg tgcggatgga tctcgtattg gagctcgggc
    12421 acgcctggct ctgcgacgac gaacgtctcg ggtggcgcct tgccgacggt ctcttgcttg
    12481 acgtcgtcgg cgctgccgcg tgcacgcagc ttcacgaccg caccgtcgag gcgcgcgaag
    12541 ccgacgatcg catccgcgag ttggcgtgcg agcggccgca agccttcgcc atagacgtag
    12601 acgcacgcgt acttgccgag cacgtcggct gcgaagcccg gcaagtcgtc gcccgcgcca
    12661 tgcacgacgc gatatgcgtg gccgtcgccg gggaggccga gctgcttgcg ccacgcgact
    12721 gctttctcga cgcgccagcc gatcaacgcg ccatcgatcg cggcgaatcc gtcggtggga
    12781 agggcgagca cgcgcagctt gccgttgtcg ggatccgcga tcgcgagacc gacgtcatcg
    12841 ccgttcgcgt cgacgagctt gacgtgatcg ccggcagcca acgcgggcag ctcacccgcg
    12901 cgagcgatat cgagatcgcg cccaccgttg gcgatgaaat tcgccgcggc cgacgacagc
    12961 gtcgcgagct tcactacaag atcacgacgg gcgcgcaggt cgaaacaccc gcgccgacga
    13021 tcgtgcggcc cgtagcgggc gtcatcgtcg tgatcacgcc cgtgtcgcct ttgacttgat
    13081 agacggtcgt cttcgtctcg ttgctcttct cgaggaagat ccagtagtcg ccgccccaga
    13141 acgcgaatgc ccacgcggcg ggtgtgcctg caagcgaggg caggttgtac gtcttgatcg
    13201 gtgcgccggt ggtcttgttg atctgctcga tgcgcggcgc gctcgcgtcg gggaaccagc
    13261 cccacagctc cgcgttgccg ttgccggtca gctccggcca gccctgcacg gtaccgagcg
    13321 gcgcagctgt gaacgacgtc gtcgagaggg tcgcgagctt ggaggtcggc gatgacatcg
    13381 ggtcgcccgc gccgcccgcg atgaagagcg tgtcgtcggt cgatccggcg gtgttcgccg
    13441 agaagcccat gccgaacagc atctcgcctt gctgcgtggt ccacgtggtg ctcgtgcacg
    13501 cggcgttcga ggtgtcgacg cggaacagct tgccgtcgga gaacagcacc cacgcaacgg
    13561 cgtcgcggtc gatgcccatc gagaaaggtt gacccgcgaa caggccgccg ttttcgccgg
    13621 gacagctcag cgtgccgatg tccatgaacg tcttcgtcat cggatcgaac ttcgaaaact
    13681 tgttgttcga gtcgacgacg tagacgagct tcgcggcgtc cgagcagcca tccgcgttgc
    13741 ccgagcccga gcccgagccg gacccggagc cgctgccggt gccgtcgtcg tcgtccccgc
    13801 gctccggccc aggaccacag gcggcaactg cgagaaatgc ggcgaggaca gcttgggtgc
    13861 gcatgggcgc cgacgctagc acgaggtgcg gaacgcctga ggtacatttc caatgtgggg
    13921 caaccggagg agccttcgac ggagctgctg ccggccgcgg gtgaggcgca ggcgcagggc
    13981 attgcacatc cgcaggttac gccagagggc cagcccgaac cgacgttgat tgggcgcacg
    14041 ctcggccgct tcacggtcgt cgagaagctc ggcaggggcg ggtccggaga agtgtttcgg
    14101 gcggagcagc agcagctcgg caggtccgcg gtgatcaagg tcttgcgacg cgaggtcgca
    14161 atggcgccga accgcgtcga gcgatttctc cgcgaggcca agctcgcatc gcgactcgac
    14221 cacccgtacg ccgcgcacat ctacgcgttc ggcgccgaga ccgacaacgt gctgtggatc
    14281 gcgatggagc acgtcaaggg cgtcacgctc gacgatctcg tcgcacgccg cggcgcgatg
    14341 cccgcgcagg tcttcgcgcc gctgttcgtg cggctctgcg aagtgatcca caccgcgcac
    14401 gagctcgata tcgtgcatcg cgacatcaag ggcgcgaacg tgatggtgat cgagcgcgcc
    14461 ggtcagctac tgccgaagct gctcgacttc ggcatcgcga agggcgacgc atttgcgacg
    14521 acgccgggtg tcgtcgacgg cgagctgacc ggacatggcg tgacgctcgg ctcgcctcat
    14581 tacatgtcgc cggagcaatg ggagcggccg gccgatgtcg acgcgcgcag cgacatctat
    14641 gcgcttggcg tgctcgcgta tcgctgcctc gccgggttct tgccgtttca cacgacgagc
    14701 aggctgcagc tcgcgcacgc gcacacgcac ctgccggtgc cggcaatgcc cgacttcgtc
    14761 tcgccgagct tggcggacgt gatcatgaag gcgctcgcga agcagcccga gcagcgatgg
    14821 cagactgccg tcgcgtttgc agaagcggtg cagcgcgccg tcggcacacc ggtgaccgaa
    14881 gccgtaccgc tgttcgatcc agcgacgcgc gacgcgtggc tcagcgcagg gccgcagccg
    14941 atcgccgacg cggtggcgca cgtgacgtcg gcgacgacca ccgtcgaggt cgatgcggcg
    15001 ctgcgcgagc tcgtcgcgat cacctgtcgc tggctcgcgg tgctctcgct ctccgggttg
    15061 ccgcccgcga cgggaactga tcccagcgtg cgcgagcatg cgcgtgcggt cgtcggtcgc
    15121 gatgatggtg cgccgtggct gcgactcgcg aaggcggccg tcgctgcgtc gccgactcca
    15181 ttgatggcac tcgtcgcggc gctcgaagca tccgagcctc tcgcgaagct cgccgatcgc
    15241 ctcgacgatc cgcatcgcca gcgcacggct gcgttgctcg cagcagacgt cggcgccgcc
    15301 gcagaagcgc tgagcgcact ggagccgttg ctcgcgtatc acctcgtgct cggtggcgcg
    15361 ggtgccgtcg cggagagctg gcagggaccg cgtcgtcgcg atcgcgagaa ggtgctcgtg
    15421 tggggagagc tcgccgagag cgaggtcgcg ctgctcgata cggcgggaaa agtcgtggcg
    15481 cgtctgtcgc cgtacgcgca ggtgatcgcg ccgctgccgg cggcagagcc ggagctgttc
    15541 ctgctatggc gaagcgggcg agggcaggcg cgcctcgtcg ctgcaccgtg gggcttcgag
    15601 cgcgacgacg aacgcgcggg cacgatgctc gctgcgctgt cgacggaaga ttccgacacg
    15661 gcgatggacg cgcaggacga gcgctcgccg tatccaggcc tcgcggcgta cggcaccgcc
    15721 gatgccgatc acttcgtcgg acgcgagcgc gaagtcgaga cgctcgcgaa ccggctcgtg
    15781 cgcgcgccgc tgatcgcggt gctcggtccg tcgggcgtcg gcaagagctc gttcatccac
    15841 gctggtctcg tgccgcgcct cggcgagcag catcgcgtgc tgacgatgcg tccgggtcgc
    15901 cacccgttgc acgcgctcgc tgcattgccg ccggtgagtg gtgacagcca cgacgaaggt
    15961 gcgctcgtcg cgcgattgcg agagctcggc gagagtgcgc agcgcggatt ggtcctcgtc
    16021 gtcgatcagc tcgaagagat ggtgacgctg tgcgcggatc ccgacgagcg ccgacggttc
    16081 gcagagaccc tcgcgggcgc ggccgatggt ccgaatgctc cggtccgggt cgtcgcaacg
    16141 ctgcgcgatg acttcgcgac ggtgatcgag agcgaagccg cattccgggg gcagttcgac
    16201 gtcttcgtcc tcgcgacgcc gccgccggag tcgctccgcc ggatcgtcgt cgagcccgcg
    16261 cgccgctcgg ccgtcagtgt cgatacgcgc gtcgtcgatg acatggtcgc cgaagttgcg
    16321 ggacgtccgg cctcgctgcc actgctctcg ttcaccgcgt cgcagctgtg gcaaacgcgc
    16381 gatcggcagg cgcgcaagat cacgtacgac gcgtacctcg agctcggcgg tgtcgccggt
    16441 gcgctggcga cgtacgccga tcaggtctac ggcagcctcg cgcgtcgcga tcaggaaacg
    16501 gtacgcgatg tgttcgcccg tctcgtcgct gcgaatggca cgcgcgtgcc gtcaccgcgc
    16561 agcgatctcg agcagcttcc cggcgcgaaa ggcgtgctcg gacatctcat cgatgcgcgc
    16621 ctcctcgtcg tgcgcgacgt cgagggccaa gacatcgtcg agatcgtgca cgagtgtctc
    16681 gccgaacgct ggccacggct cgcgcgctgg cgcagtgaag atgccgcgga tcgcgcgctc
    16741 ctcgcggacg tcacgaccgc atcgcgtcgc tggatcgaag ccgggcgccg cgccgacttg
    16801 ttgtggcgag gtgaagccct cgcggagctg tcgcgtctga ctgctcggtc gacagcgatg
    16861 accggcgacg aacgcgcgtt cgccgatgcc gcggttttgg cgcagacacg cgctcgccgg
    16921 attcgtcgca cgttgatcgc agcgagcatg ctcgtgctcg ccggtgtcgc gatcgtgatg
    16981 gcgtacctca gcgtcgtcgc gaacgagaac cgcgcagagg cggagcgcag cgccaagaag
    17041 gccgccgacg ccgcgagcct tgccgagcag cggttgacgc agagcctgat cgcgcaaggt
    17101 cgccgcgagc tcaacgatgg tcgcgacatg ccggcgctcg cgtatttcgc ggcggcgctc
    17161 gagcgaggcg ccgatacgcc tgcattgcgt gaaatggtgt cgctcgccga gcgaggctgg
    17221 ccgtacttcc tcacgacgca tcgtggaacg gccggtggcc gtgcgatcgt gacgatggcg
    17281 ggatcgcccg atggttggat cgtcggtggc gacgattcag gttctctccg ctggtggaac
    17341 gacgaaggtg cgctcatcgg tgagctgaag acggaactcc agtcgatcac cggcatcgat
    17401 cgcaaccccg ataattcgct cgtcgtcagc ggccttcgcg gcatcgtgat cgtcgacgca
    17461 tcgcatcgca tcgtgcagaa gctcgcgatc gctgacgagc cgtggttcgc gcgtcgtggt
    17521 cccggccccg acgaggtgag cgcgctcgcc agtgatgctc ttcgcgtcta cggcttggac
    17581 ggcgtggagc gtcgctcgat gccgatcacg cgcacgggag acacgcagga gccgatgctc
    17641 gacgagggag cgcgccacgc gatcttcgcc gcggatggcg agctgtcggt cgtcgatctg
    17701 aagacgatga agattcgctc cctcgcgcat ggcatccaca acgagccaaa tggcgcgaac
    17761 gatggatcgc gatacgccta tatcgaccac gactggaacg ttcacctgct gcgcggcgac
    17821 gggacggaga tcaaaaagat caagccggac acgcggccga ttcgcctcgt cctctccgac
    17881 gacggcacgc gtctcgggat cacgagcgag caacgcttcg atgtcttcga cgagaagggc
    17941 gagctcctcg acgggattcc gctgaagctc gagcaggcga gctacgcaat gcgcggcgac
    18001 gatgtctggg tgacgaatca ggaagggacg gttcgtcact acgtcgaggg ccagctcgtc
    18061 gcttcagcac cgctccatgc aagcgagatc cgcctggtcc tcgtcgcgcg caatgcactg
    18121 gcgaccgtcg gaagcgactc gacgctcgtc atgatgaagg cggatgcgcg gcaagtcgtc
    18181 gagatgcccc acctctgcaa gcacatctcg ttcgcacagt acggcgttgc gactggctac
    18241 acgtgcggcg acaccacact gctgtacacg gggcgcaaga agctcggcga gtatccggag
    18301 accatgatga tggtgcaggt catgcacgag ccggcgtatg accgcacggc gctcgccgcc
    18361 gatcgaggta tccgcgtctt cgatggtgac gcgaaggaga tcgcgcgtac ggataagttc
    18421 ctcggagccg cgtgggccga tcgcgatcac ctgtgggccg tgacggatcg caagacgctg
    18481 tcgcggtggg cgttcgccac tgacacctgg gagaagatcg gcgaagttcc agagagctcc
    18541 gcagtcggct cgattcccgg ctatctgctg atcgggaccg aggacggcga ggtccatgtc
    18601 ctcgtcgatg gccgcgagca gcacgtcgtc gccatcggta cgccggtcgt tggcttcaat
    18661 gcgtcaccgg atcaccggtg ggtcgccgcg cagctctcca gtggcgccgc agcgatcatc
    18721 agcacgacga cctggacgct cgagcgaacg ctcgcgcagg gtgacgcgaa cggtgatttt
    18781 ccgatcttcg atgcgacagg agatctcgtg ctgcgggcga accactacgc gttgacgatc
    18841 tgggaccgcg ccaccggcga ggagctggtt tttggcctcg acctcctatc ggacctcggc
    18901 gggggccgtt ggctgccgga tggccgcatc gaaatgaacc gaagaaggcc gagcctcctc
    18961 gatattcctc gcgacaagcg tccggtccct gacatcctcg cggacatcgc atgccatgtc
    19021 ccgctcaagg tcgttggcag ccggatcgag cccacggtcc ccatcgcttg cgccgcggga
    19081 gtatccggcc ttacgctacc taagtgatcg acgacgacgc tggctcaacg accgaacaga
    19141 tcgatcgtca gactccgttt ggcgacaggg tcctcctcgt catcgacggc gcatcactcc
    19201 aagtcgtgcc gctgccaccg accggaacgc tcacggttgg ccggtcgcag aagtgcgaca
    19261 tctcgatcga ctcgggctcg gtgtcgcgcc atcacgcaaa cgtcgtcgtc ggcaacgatg
    19321 tcgaagtcga agacgtcggc agctcgaacg gcacgttcgt cgatggcgtg cgcctgcccg
    19381 gcaacaagaa ggtccgcgtc ggtgtcggca tcccgttcct gatcggcggc gtcacggtga
    19441 tggtgcaaac gcgcgcgggt cagcgccgcg acagcgcctc caagtcgagt gcgctcgccg
    19501 ctctcgagca atccgcatcg cgcatcgccg tcggcaagct ctccgtcctg atcctcggcg
    19561 agacgggcgt cggcaaagag cgcttcgccg agcgtctgca cgagatgtcg ccgcgccgct
    19621 cgaacgcgtt cgtgcgcatc aactgcgccg cgatctccga gccgctcctc gaagcggagt
    19681 tgttcggcaa tgaagccacg aacaagcccg gcctcatcga gtacgccgat ggcggcaccg
    19741 cgttcctcaa cgacgtggcc gagcttccgc agtccctaca agtaaagctc ctgcgcgtga
    19801 tcgaggacaa cgcggtccgt cgcatcggct cgccgaaccc gcgcaacgtc gacgtccgct
    19861 tcctcgcagc gacgaacaaa gatctcgccg ctgaagtcga cgcgggtcgc ttccgccgtg
    19921 acctctactt ccgcctcgcc ggcgcgtcgt tcacgatccc gccgttgcgc gaccgcaaag
    19981 atgaagtgct gccgctcgcc gagcaattcg tggcttcggc ctcgggcccg ctcggccgcc
    20041 acttcgtgct cgccgacgac gccaagcagt ggctcgccgc gcacgactgg ccgggcaaca
    20101 tccgcgagct gcgcaacgcg tgcgagcgcg ccgttctgct cgcgaccggc tcgatgatcg
    20161 agaagcagca cctgacgatc gacgagccga agcgcccgcc aggtcgcttc cgctctccga
    20221 cgactccgcc gcccggcatg atcacgtcgg atatgccgac gcaggtccgc gcgacggtgg
    20281 ccgagctcga gaagcagcgc atcctcgagg cgctcgacaa gtgcgccggc aatcaaacgc
    20341 gcgccgcaga gctgctcggt atctcgcgcc gcacgctgat caatcggctc gacgaatacg
    20401 gcatcgctcg cccgcgcaag cgcgacgagt agcgcgcgca gcgttctact acttgcgctc
    20461 gaggatgatc agctcgaggc gctcgttgat gtctttggcg cccttcgact tcggatcgac
    20521 gagcggcttg gtgccgccga agccgcgcgg ctcgaggcgc ttctcgtcga tgccgtactt
    20581 gatcaagtac tcgcggatcg cgaacgcgcg tagctcgctg aggcgctgat cagcctcggc
    20641 gttcttcgtg ggctggacgt gcatcgtgat gcgaaggcgc aggatctcgg ggtgagcgcg
    20701 gagctgcgcg ccgacctgat tgagtaggtt gaagctgtcc ttttggatga tcgtcttctt
    20761 gaagacgacg ttctcgagga gctcgaggcg gtcggggctg atcacgacga gcgcgttgcc
    20821 gcgatcgggg cagccgtctt catcctcggt gccgttgatg acttccgctt cgagcgggca
    20881 cttgtcctgg gcgtcgagga acgagtcctt gtcgttgtcg agctcggggc agccgtcgag
    20941 gtcctggaag ccgtctttgt cttcggcttc gcgcaggcac ttgtcttgtt tatccggcac
    21001 gccgtcggcg tcgtcgtcgg gatcggggca gccgtcctcg tcggcgttgc cgtccttgtc
    21061 ttcgggctcg agcgggcatt tgtcggtcgt gtcgaacacg ccgtcggcgt cgttgtccgg
    21121 atcggggcag ccgtccgtgt cgtcgaagag gtcgatgtct tcggcttggt tggggcactt
    21181 gtcgacgtcg tcgaacatgc cgtcgccgtc gctgtcgacc ttcggcggtg gcggcggtgg
    21241 cggcggctcg ggcggcgcct tcgacgggcg gaacgagatg ccgaacatga attccgcgta
    21301 cgacgcatcg agcgtgtacg gcgctttcgc cgatgcaccg cgcaggaagc ggccttcagc
    21361 gcgcagcgtg atcgagcgat ggacgagaaa ccgtgcaccg atatctgtgt tgagcggcca
    21421 gtcggtatca ctgccgagca cgtcgctcga cgtgtgcatg aagccatcgc cgatcgtcac
    21481 gaacggcacg acgtatggcg agaggttcgg atactggaag atcagctgga ggtggccgct
    21541 gatgatcttc gcggagcctt gtggt
//
LOCUS       DATLBZ010000531         7310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9635309, whole genome shotgun sequence.
ACCESSION   DATLBZ010000531 DATLBZ010000000
VERSION     DATLBZ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9635309"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VMZ53_26655"
     CDS             <1..434
                     /locus_tag="VMZ53_26655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32120.1"
                     /translation="THLPSAFGVAGVIASDETAFGVVGAAFADDQLPSQTRPAPSTPP
                     SEMAPADVQKWLSFFDKLVSTVVNTQSSCDKMATDVNAVVDANKDAIALAKRAKAQGK
                     KLPADAKAKMLDGVKKMLPGMQNCGTNEKVRAAFAKLDLNH"
     gene            456..1055
                     /locus_tag="VMZ53_26660"
     CDS             456..1055
                     /locus_tag="VMZ53_26660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HUS32121.1"
                     /translation="MSGSWMLRQLDYVTRPHHVPADAGRLALFNKGVTPERYVDFLSR
                     LYSFEAPIEARWKRMPELATVLDVERRIRTPFLLADLHALGAQPELLPVPNFIGIEQA
                     LGWMYVVERGRRLNGMLYRHLQRRLPREAMLGGNYLVASTSSGARWEELGRTLDKVAN
                     NAVKTDQIVNAANRAFRALRMTPIASSAAPAAPAPRSAA"
     gene            complement(1109..2491)
                     /locus_tag="VMZ53_26665"
     CDS             complement(1109..2491)
                     /locus_tag="VMZ53_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS32122.1"
                     /translation="MARILVADDEPGLREFVSDALELDEHVVVQAPDGKAAAKLLDER
                     GFDLVITDLKMPGLDGMALLRKVRTEQPEVEVIVMTAHGTVDNAVEAMKLGAFEYLQK
                     PLSGPDELRLLVSRAAERRGLRDHVDGQARHAEAGAGGIPLTYGDPTMKPVVDAIDKV
                     ARTHATVLLLGESGTGKEVAARNIHALGTRATRPFMAINCAALSEQLLESELFGHEKG
                     AFTGATERKRGRLELADGGTFFLDEVGELKPELQAKLLRVLQEKRFERVGGTRTLEVD
                     VRWIAATNRDLRAMVDAGTFRDDLYHRLAVFPIRLPPLRDRRKDLLPIAKSLLVRIAA
                     DLKRPLPALTQAAQERLLGAVWRGNVRELANALERAAILADGDAIDASHVWLDEAPAP
                     RGSSSGGSGGRGGSGGATTGEIRPLAELEREAILHALAVTDGNRRKAAELLGIGERTL
                     YDKLKKYDAE"
     gene            complement(2646..4160)
                     /locus_tag="VMZ53_26670"
     CDS             complement(2646..4160)
                     /locus_tag="VMZ53_26670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS32123.1"
                     /translation="MGASSASSASNASGPSRSSSSGGRRVPPLAIAIVLVAAALVATA
                     WSTRSTTVDAFAAVRDGQALAVQQSVRADLADLGGPPTAADLDAIVREHKPDGLRYLA
                     FLDGRGRVHVAAGTPTIERPPRLDRTGMHLEHVGGRVRIEVRGQLRRAWGNGRGALWL
                     AMEIESPKAGALRSAATRTLAIGLIAAFMLLVVAAWLVRAELRRSAQERKRERERRLA
                     SLGEMSAVLAHEIRNPLASLKGNAQLLASSLPRGEKSRTKAERVVDEAIRLEKLTQDL
                     LAFVRTGELVRRDVSPTKIAKDALAAHATTNRMPSSDRVPREPDATRTSESASLQLET
                     EATRTPDSENLQLETEATRTPDSENLKLETEATRTGESDNLRLETEAEVALEVANAKL
                     DVANAPLTWSLDAARIREVLINLVDNALAAGGPVEVRVAERAGRLVIEVRDHGPGVPA
                     AERDKIFEPFHTGKTRGTGLGLAIAQRIVELHRGTITVDDAPGGGALFRIEIPR"
     gene            complement(4325..4771)
                     /locus_tag="VMZ53_26675"
     CDS             complement(4325..4771)
                     /locus_tag="VMZ53_26675"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HUS32124.1"
                     /translation="MFTKTKIAIVLVGSLIAGVAGAQGLRGHGRGQMIEKFDTNKDGK
                     LDDSEKAAAKTAFEAQHAAKKKEMLTKFDTNKDGKIDDTERGVARDARMTERFKKLDT
                     DGNGQVSLAEFKANKLAKQGRGGRMHHRGGHAFGKGRGMGQGKGTK"
     gene            4937..5788
                     /locus_tag="VMZ53_26680"
     CDS             4937..5788
                     /locus_tag="VMZ53_26680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32125.1"
                     /translation="MKTTLALLVCLLAAGCGAKMKMASASMAPQAAAMAPAAAPPPLD
                     RSVFARDPNGQLSEEMLQKILASPIELDLPSRVGVLPIITATDWRGPSPDYEHVPAGT
                     SPMVARLRKDAAFSLVTEMMPIPSGSLGMEALREAAARYRLRYVILYREVLAKKNYQN
                     KWTWGYATFVGALFLPGKQHEVYGYIEATMFDVKTGLLMFTTRRSIMATKNSNEWHTK
                     DKLSQLASNAVYKFAPDLAQDFLLDVRRFANAAVAENARKLGQPELVQVPGVGPTIAT
                     DAPTAVN"
     gene            complement(5772..6908)
                     /locus_tag="VMZ53_26685"
     CDS             complement(5772..6908)
                     /locus_tag="VMZ53_26685"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HUS32126.1"
                     /translation="MSGAASDLRVVGLQALVASIALIALAWIPPMPVAGAQLSDAIGA
                     AGVGLVLLLVRPRVGWVVAAMAIYVALAAVSCMVMHGSFAKLGGVAWLVMLALGVAAA
                     CEDPAVEARIRKALVVAALVGAVTGLVGAVLFFVGVKTGLLNHAGDLVPGNYPRIRGT
                     MMRANALAGLIGTGLLLVGDLDARWRRPAYAVLLVALVFTFSRTWIALAGTVAVTHVA
                     FVRRRDVLALAAAVLTVAVMLLVSWPTLQLDPSHPWRISFSGEPGTRLVHLRDALDTI
                     RAHPLLGVGPGHAAAAGGWDAHFTLANVAAVVGIPAALAFLAILVGAGREVWKRARAG
                     DLASRALASIFILYAFDALARDVEDQRALWIVVGLALARFSSRR"
     gene            complement(6915..>7310)
                     /locus_tag="VMZ53_26690"
     CDS             complement(6915..>7310)
                     /locus_tag="VMZ53_26690"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUS32127.1"
                     /translation="FKAVACAISMLQGLEEFNRTRAAENQTPIRIGIGVNTGNVITGS
                     IGSTRALQYTAIGDAMNVASRLVNVAASGEIIISEHTYRHVAGRIEAIQLPPVKVKGK
                     AEELKVYRVTGLRNIQTPIPGDWSGPTNG"
BASE COUNT         1175 a         2484 c         2448 g         1203 t
ORIGIN      
        1 caacgcatct gccgtcggcg ttcggtgtcg ccggagtgat tgcgtccgac gagaccgcgt
       61 tcggcgtcgt cggcgccgcg ttcgcggatg accagctgcc gtcgcagacg cgccctgctc
      121 cgtcgacgcc gccatccgag atggcgcctg ccgacgtgca gaagtggctc tcgttcttcg
      181 acaagctcgt gtcgacggtc gtcaacacgc agtcgagctg cgacaagatg gcgacggacg
      241 tcaacgccgt cgtcgacgcg aacaaggacg cgatcgcact cgcgaagcgc gcgaaggctc
      301 aaggcaagaa gctgccggcc gacgcaaagg cgaagatgct cgacggcgtc aagaagatgc
      361 tgccgggtat gcagaactgc ggaacgaacg agaaggtccg tgcggccttc gcgaagctcg
      421 acctcaacca ctgaacaaag atcgaagagg acatcatgtc cggctcgtgg atgctgcggc
      481 agctcgatta cgtcacgcgt ccccatcatg ttcctgcgga cgccggacgg ctcgctctct
      541 tcaacaaagg cgtaacgccc gagcgctacg tcgactttct ctcgcgcctg tactcgttcg
      601 aagcgccgat cgaagcgcgc tggaaacgaa tgcccgagct cgctacggtg ctcgacgtcg
      661 agcgccggat tcggacgcca ttcctgctcg cggacctgca cgccctcggc gctcagcccg
      721 agctgttgcc cgtcccgaac ttcatcggca tcgagcaagc gctcggctgg atgtacgtcg
      781 tcgagcgcgg tcgccggctc aacgggatgc tgtaccggca tctgcagcgg cgcctcccgc
      841 gcgaagcgat gctcggcggc aactacctcg tagcgtcgac gtccagtggc gcgcgctggg
      901 aagagcttgg ccggacgctc gacaaggtcg cgaacaacgc cgtgaagacc gaccagatcg
      961 tcaacgccgc gaaccgcgcg ttccgcgcgc tgcgcatgac gccgatcgcg agcagcgccg
     1021 cacccgcagc acccgctccg cgcagcgccg cgtagccaac agccggcgac gactcgactg
     1081 ctccaaattg aagcagtggg gtcggaccct attcggcgtc gtacttcttc agcttgtcgt
     1141 agagcgtgcg ttcgccgatg ccgaggagct cggcagcttt gcggcgattg ccgtccgtca
     1201 cggcgagcgc gtggaggatc gcttcgcgtt cgagctctgc gagtggccgg atttcgccgg
     1261 tggtcgctcc cccagagccg ccacggccgc cggatccacc cgagctcgag ccgcgaggcg
     1321 ccggcgcttc gtcgagccag acgtgcgagg cgtcgatcgc atcgccgtcg gcaaggatcg
     1381 ctgcacgctc gagagcgttc gcgagctcgc gcacgttgcc tcgccatacc gcgccgagga
     1441 ggcgctcctg tgcggcttgc gtgagcgcgg ggaggggccg cttgagatcg gcggcgatgc
     1501 gaacgagtag gctcttcgcg atcgggagca gatccttgcg gcggtcgcgc agcggcggga
     1561 gccggatcgg gaagaccgcg aggcggtgat agagatcgtc gcggaacgtg cccgcatcga
     1621 ccatggcgcg caggtcgcga ttcgtcgcgg cgatccagcg aacgtcgacc tcgagcgtgc
     1681 gcgtgccacc gacgcgttcg aagcgcttct cctgcaggac gcgcaggagc ttggcctgta
     1741 gctcgggctt cagctcgccg acttcgtcga ggaagaacgt gccgccgtcg gcgagctcga
     1801 gacgaccgcg cttgcgttcg gtcgcgcccg tgaacgcgcc cttctcgtgg ccgaacagct
     1861 cgctctcgag gagctgctcg gagagggccg cgcagttgat cgccatgaac gggcgcgtcg
     1921 cgcgcgtgcc gagcgcgtgg atattgcgcg cagcgacttc cttgccggtg ccactctcgc
     1981 cgaggagcaa aaccgtggcg tgcgtgcgcg cgactttgtc gatggcgtcg acgacgggct
     2041 tcatcgtcgg atcgccatac gtcagcggga tgccaccggc gccggcttcg gcgtggcgcg
     2101 cttggccgtc gacgtgatcg cgcagaccgc ggcgctccgc cgcgcgcgag acgagaaggc
     2161 gcagctcgtc ggggcccgac agcggcttct gcagatactc gaacgcgccg agcttcatcg
     2221 cctcgactgc gttgtcgacg gtgccgtgcg cggtcatgac gatcacctcg acttcgggct
     2281 gctccgtgcg gaccttgcga agcagcgcca tgccgtcgag gcccggcatc ttgaggtcgg
     2341 tgatcacgag atcgaagcct cgctcgtcga ggagcttggc ggcggccttg ccgtcgggcg
     2401 cctgcacgac gacatgctcg tcgagctcga gcgcgtcgga aacgaactcg cgcaggcctg
     2461 gctcgtcgtc ggcaacgaga atgcgggcca tcagccgccc tcgcagtcgg caacctggat
     2521 gcgggccatc agccgacctc gccgatctcc ggtcgctccg ccgtgcccga gtgtggaggc
     2581 ggtgcaccga cctccgatct cgctggtcgc tgcgatcggc gcgagtgtgg agtgatgggc
     2641 ccggctcatc gcggaatctc gatgcggaag agtgccccac cgccgggtgc gtcgtcgacg
     2701 gtgatcgtgc ctcgatgtag ctcgacgatg cgttgcgcga tggccaggcc gaggcccgtg
     2761 ccgcgcgtct tgccggtatg gaacggctcg aagatcttgt cgcgttcggc cgcgggaacg
     2821 cccgggccgt ggtcgcgcac ttcgatcacg aggcgccctg cccgctccgc gacgcgtacc
     2881 tcgacgggcc caccggccgc cagcgcattg tcgacgaggt tgatgaggac ctcgcggatg
     2941 cgcgcggcgt cgagcgacca cgtcagcggc gcgttcgcga cgtcgagctt ggcgttcgcg
     3001 acctctagcg cgacctccgc ctcggtctcg agccgcaggt tgtcgctttc gcccgtgcgg
     3061 gtcgcttcgg tctcgagctt caggttctcg ctatcgggcg tgcgggtcgc ttcggtctcg
     3121 agctgcaggt tctcgctatc gggcgtgcgg gtcgcttcgg tctcgagctg caggcttgcg
     3181 ctttcggacg tgcgggtcgc gtcgggttcg cgcggtaccc tgtcgctgct cggcatgcga
     3241 ttcgtggtcg cgtgggcggc gagtgcatcc ttcgcgatct tcgttggcga gacgtcacgg
     3301 cgaacgagct cacctgtgcg cacgaatgcg agcaggtcct gcgtgagctt ctcgaggcga
     3361 atcgcctcgt cgacgacgcg ctccgccttc gtgcgcgact tctcaccgcg cggcaagctc
     3421 gacgcgagca gctgcgcgtt gcctttcagc gacgccaacg gattgcgaat ctcgtgggcg
     3481 agcaccgcgc tcatttcgcc gaggctggcg aggcgtcgct cgcgctcgcg cttgcgttcc
     3541 tgcgcgctgc gacgcagctc cgcgcgcacg agccacgcgg ccacgacgag cagcatgaat
     3601 gcggcgatga gaccgatcgc cagcgtacgc gtggcggcac tccgaagagc gcctgccttc
     3661 ggtgactcga tctccatcgc cagccagagc gcgccgcggc cgttgcccca agcgcggcgc
     3721 agctggccgc gcacctcgat gcgaacacga ccgccgacgt gctcgagatg catgcccgtt
     3781 cgatcgaggc gcggtggccg ttcgatcgtg ggcgtaccgg cagcgacgtg gacgcggccg
     3841 cgaccgtcga ggaatgcgag gtagcgcagg ccgtcgggtt tgtgctcgcg gacgatcgcg
     3901 tcgaggtccg cggcggtggg cgggccgccg aggtccgcga ggtctgcgcg caccgactgc
     3961 tgaaccgcga gcgcctggcc atcgcgaacc gccgcgaacg catcgacggt tgtcgagcgc
     4021 gtcgaccacg ccgtggcgac gagcgccgcc gccacgagca cgatcgcgat cgccagcggc
     4081 ggcacgcgac ggccacccga cgacgacgag cgcgacgggc ctgatgcatt cgatgcgctg
     4141 gatgcgctcg atgcgcccat ggctggcagc ataccgctac ctcgcgctct cgctgaagct
     4201 cgtcacgtcg aaccgccgct ggcgatcgtc cacccgcata cgccgctgac gatcgtccac
     4261 ccgcatgcgc cgctggcgat cgtccacccg catgcgccgc tggcgagcgt ccaccgcgcg
     4321 ctgcttactt cgtgcccttg ccctggccca tgccgcggcc cttgccgaag gcatgaccac
     4381 cgcgatgatg catgcgaccg ccgcgtcctt gcttcgccag cttgtttgcc ttgaattcgg
     4441 cgagcgagac ctggccgttg ccgtcggtgt cgagcttctt gaacctctcg gtcatgcgcg
     4501 cgtcgcgagc aacaccgcgc tcggtgtcgt cgatcttgcc gtccttgttc gtgtcgaact
     4561 tggtcagcat ctccttcttc tttgcggcgt gctgcgcttc gaacgcggtc ttggcggcgg
     4621 ccttctcgct atcgtcgagc ttgccgtcct tgttggtgtc gaatttctcg atcatctggc
     4681 cgcggccgtg accgcgcagg ccctgtgcac cggcaacacc ggcgatgagc gaaccaacga
     4741 gcacgatcgc aatcttcgtc ttcgtaaaca tggctgagag acctcctggt ctggtccctc
     4801 cgtagtgcga cgcgcgtgcc gtcggcaact gggcgattct cctcggacgc tctgcaccta
     4861 cactgcggag tctgcagatc cgcctgcgaa aactgcagcc ctgtaagcga cacgcgatcg
     4921 tctcgtttgc ttgggcatga agacgacact cgctttgctc gtttgcctac tcgccgctgg
     4981 atgtggcgcc aagatgaaga tggcgtcggc gtcgatggct ccgcaggccg cagcgatggc
     5041 tccggctgct gcacctcctc cactcgatcg cagcgtgttc gcgcgcgatc ccaatgggca
     5101 gctctccgaa gagatgctgc agaagattct cgcgtcgccg atcgagctcg atctgccgtc
     5161 gcgtgtcggc gtgttgccga tcatcacggc cacggattgg cgcggcccga gcccggacta
     5221 cgagcatgtg ccggcgggta cgtcgccgat ggtggcgcgc ctgcgcaagg acgccgcctt
     5281 ctcgctcgtc accgagatga tgccgattcc gtcgggctcg ctcggcatgg aagcgctgcg
     5341 tgaagcggcc gcgcgctatc gcctgcgcta cgtgatcctc tatcgcgagg tgctcgcgaa
     5401 gaagaattac cagaacaagt ggacgtgggg ctacgcgacg ttcgtcggcg cgctgttcct
     5461 cccgggcaag cagcacgagg tttatggcta catcgaggcc acgatgttcg acgtgaagac
     5521 gggcctgctc atgttcacga cgcgccgctc gatcatggcg acgaagaact ccaacgagtg
     5581 gcacacgaag gacaagctct cgcagctcgc gtcgaatgcg gtctacaagt tcgcgccgga
     5641 tctcgcgcaa gacttcctgc tcgacgtgcg tcggttcgca aatgcggcgg tcgccgagaa
     5701 tgcgcgcaag ctcggtcagc cggagctcgt gcaagttcct ggcgtcggac ccacgatcgc
     5761 gacggacgcg cctaccgccg tgaactgaag cgcgcgagcg cgaggccgac aacgatccac
     5821 agcgcgcgtt gatcctcgac atcgcgagct agcgcgtcga acgcatacag aatgaagatc
     5881 gacgcgagcg cgcgggaggc caggtctcct gcgcgtgcgc gtttccagac ctcgcgaccc
     5941 gcgccgacga ggattgcgag gaacgcgagt gcggcaggaa tgccgacgac cgcggcgaca
     6001 tttgcgagcg tgaagtgcgc atcccagcct ccggctgctg ccgcatgccc gggcccgacg
     6061 ccgaggagtg gatgcgcgcg aatggtgtcg agagcgtcgc gcaaatgcac gaggcgcgtg
     6121 ccgggctcgc cggagaacga gatgcgccat ggatggctcg gatcgagctg cagtgtgggc
     6181 cacgacacga gcagcatgac tgcgaccgtc agaaccgctg cggcaagcgc gaggacatcg
     6241 cgccgccgta cgaatgcgac gtgcgtaacg gcgacggtgc ccgcgagtgc gatccacgtg
     6301 cgcgagaacg tgaagacgag cgcgacgagc agcactgcgt acgcaggacg ccgccagcgc
     6361 gcatcgagat cgccgacgag caagagcccc gtaccgatga gcccggcgag cgcgttcgcg
     6421 cgcatcatcg tgccgcgaat gcgcggatag ttgccgggca cgaggtcgcc cgcgtgattg
     6481 agcagccctg tcttcacccc gacgaagaac agcaccgcgc cgacgagccc agtcaccgcg
     6541 ccgaccagcg ccgcgacgac gagcgccttg cgaatgcgcg cttccaccgc cgggtcctcg
     6601 cacgcggcag cgacgccgag cgccagcatc accagccacg caacgccgcc gagcttcgcg
     6661 aagctcccgt gcatcaccat gcaggagaca gccgcgagcg cgacgtagat cgccatcgcc
     6721 gccaccaccc agccaacgcg cggccgcacg aggagcagca ccaggcccac gccagccgca
     6781 ccgatcgcat cagaaagctg cgcgcccgcc accggcatcg gcggaatcca ggccaacgcg
     6841 atgagcgcga tgctcgcgac gagcgcttgc agaccgacga cgcgcaggtc cgacgccgcg
     6901 ccactcacga agagctagcc gttcgtaggg ccggaccagt cgcccggaat cggcgtctgg
     6961 atgttacgga ggccggtcac gcgatagacc ttgagctctt ccgccttgcc cttcactttg
     7021 acgggcggca gttgtatcgc ttcgatgcga cctgcaacgt gccgatacgt gtgctccgag
     7081 atgatgatct cgcccgacgc agccacgttc acgagacgcg aggcgacgtt catcgcatca
     7141 ccgatcgccg tgtactgcag cgcgcgtgtc gaaccgatcg acccggtaat gacgttgccc
     7201 gtattcacgc cgatgccgat gcgaatcggc gtctgattct ccgcagcgcg cgtgcggttg
     7261 aactcctcga ggccctgcag catcgagatc gcacacgcga ccgccttgaa
//
LOCUS       DATLBZ010000532         8314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10788203, whole genome shotgun sequence.
ACCESSION   DATLBZ010000532 DATLBZ010000000
VERSION     DATLBZ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10788203"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VMZ53_26695"
     CDS             <1..519
                     /locus_tag="VMZ53_26695"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HUS32128.1"
                     /translation="DANVIAGAGTLSAEICEQADALDAIVVAVGGGSQAVGALVAARA
                     LRPAVKVYGVQARAASAIHDSWHRGTRLVTESANTFADGLATRATYDLTWPALREGLA
                     GFVAVTEAQLASAIRLYIRATHNLAEGAGAAGLAGLLALRDELAGKTVAIVLSGSNID
                     GDTLRRVMSSDL"
     gene            516..1853
                     /locus_tag="VMZ53_26700"
     CDS             516..1853
                     /locus_tag="VMZ53_26700"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HUS32129.1"
                     /translation="MRRALITCAILLGSGAAHAGPLGELWRMKVRECPLYLPDTPGTL
                     AISKEPAVTLYDRATGKRVRDVKVTPKPKKSLSATRLGDTLVLQDWNVSYTGVDPKTD
                     AVRWRRDTPTKQGEKYWLLYPAGADGVLVRVDRKPTFSLELERFDGATGRPRWTAKPP
                     TARAQFETIVAGTRHTYLVTMDREPRGFTIVALDETGKPSWKIDDPIGDLPHFHVVGD
                     DLVAVVSDVVRVFAGATGKVSTWKLPWSPLYIVHGDTIYAQRASAEILAFGGATGTQR
                     WVTPIADHSGLLLPLGVSNGSLYVKDGEQLRVLDAATGKLASTYGVAGMDRLKAWDTA
                     PAITMCDTATKTLVALDPSATATDHRVTISGRIICKNCKTPPHVTVGSVTTTADASGK
                     FSLEVTARGRLSMVVTDDESLLAAWVRKITFDKDAKLKLGDVRVKMPVPNDLD"
     gene            1890..2984
                     /locus_tag="VMZ53_26705"
     CDS             1890..2984
                     /locus_tag="VMZ53_26705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32130.1"
                     /translation="MKGIVGKVVRGFVLALVCLAMFDVPASYLASRGWSPWLSLAFGL
                     LTFPILSLVWHALSEHARKKAPPLKPATRGWERFLLRSTLVGVLVIGALVALAGGKRV
                     LSGFRNHALWFIPDATSPLVADSKLFDFVPPNAEVLIWLRVTDDTRAQLGAAVPSIER
                     VGKALQAEDVVAAIGKDGFWIGEHGDAEVVGAFAELFAELQKLGKDHGLDAFTDSLAG
                     SVTKAPGNVRYWVSPGWTKAVSTGRPSALVDLFSQAPDDAFLVAVTRPAPGGIAKRDL
                     PDVTSGVVYVRLHRGRVVVAIRGDFASDTEAKKRADEVRDSTARIEDHECWSVADGRA
                     GVSQTDRTVTMELSIPLDRIESAMRCLDKK"
     gene            2981..3640
                     /locus_tag="VMZ53_26710"
     CDS             2981..3640
                     /locus_tag="VMZ53_26710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32131.1"
                     /translation="MTARGLAIAAVVALAPTIAHASPELDALVKDVPTCAARTYCFAI
                     RVHVPLDDRGAPIATAAWIAGQVAAANQHFEKLDVAFQIDGIGPLPAAAARVEDRKER
                     DSFGGRLSRKVIDWFVTGQLDDIDIPGAQVFGVTWRRGDRKFVIVSTQARERTLAHEL
                     GHFFGLPHSTYAISIMNKTPREEPPPEARTFADEEYARMKPRVKDAVRSRIVANVKRR
                     R"
     gene            complement(3646..4281)
                     /locus_tag="VMZ53_26715"
     CDS             complement(3646..4281)
                     /locus_tag="VMZ53_26715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HUS32132.1"
                     /translation="MAQSRELRGLVIVEILAIVGLAFVPLPAIVPVAVPLLVLASLSR
                     WTRGRDWGELFHGGLRATIVGATAGIVGLALALAIGTPLVEHVTDRAVEWSMYTLVRG
                     NASQLFAVAIYVAVTSLAAELALRGWIVERVLELASGRPGVTVLAILIGSAAEMIVTP
                     GNFAMRAGAGLFGAGLGMMYVAGNRSIVAPICARMAFALGALMLESMKLVG"
     gene            4405..5259
                     /locus_tag="VMZ53_26720"
     CDS             4405..5259
                     /locus_tag="VMZ53_26720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32133.1"
                     /translation="MRERIVTIGCLSALFLGLLGSAIAAAAPTDDELKTRKAELDKKL
                     AGQGFTVVVEAPFVIVGDESAATVKGRRTNFIRWTVGLIEKDFFKKRPDKLIEIWLFK
                     NITTYKRGAKKFFNDEPSTPYGYYSPDDNALVMNIGPGAGTLSHELVHPYMEANFPNV
                     PSWFNEGLASLYEQPREREGHMWGTTNWRLPGLQKGIRDKSIPDVATLLKTTRDGFYE
                     AAYDSYAMARFLCQYLQDKGKLRDFYAKFAADTKDPTGQAVLAAVLGQDLAAFDAAFR
                     KWALGLRR"
     gene            5640..7172
                     /locus_tag="VMZ53_26725"
     CDS             5640..7172
                     /locus_tag="VMZ53_26725"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HUS32134.1"
                     /translation="MYWLPSHSKAEKQIAGSLRPASAFFRFLWETRDELFSREFQDEL
                     VMSYQPRGQEPCAPALLAMVMLLQRYTGLSDADAVDAAENDRRWQLVLGCLGAEKAPF
                     GQGTLVRFRTRAIANDLDKKLVERTVELARKTSRFGWKKLRVALDSSPLHGAGRVEDT
                     WNLIGRAMAKVVHAVSLALDIEEETIIKSAGLTVLSGDSIKGALDIDWNDDDAQVAAL
                     QKLLHQVDTLEQWVAKRAAKQAEVPPLKESLDLLRKVVDQDIEPDPSGSGRRIKQEVA
                     KERVISISDPEMRHGRKSKTKLFNGFKRHIAIANNLILATVVVPANIQEHEATAPLLK
                     VVEEYGDIHTLDIDRGYLPSPRVAELHRDGTVIHSRPWAPTNKGLFTKDDFSINIRRR
                     EVTCPNGKIAKAVDSGKAAFAVDDCAKCKLKPSCTTSSHRTVTLHPAEDLLVKLRRSN
                     ASKHGRAELRERVAVEHHLARIGSIQGDTARYAGARKNELDLNRSAAIANLHEVARRR
                     AA"
     gene            complement(7185..>8314)
                     /locus_tag="VMZ53_26730"
     CDS             complement(7185..>8314)
                     /locus_tag="VMZ53_26730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32135.1"
                     /translation="LPLYGVTIYVPNADPGPFMEGAQCGRCDQQIPGEPITSAKSDEA
                     GRFTMSDVPAGRDIPLIVTVGKWRKRVAIPMVNECQDNPLPPELTSLPTSRQEGDIPK
                     MAIATGSCDALECLVRKLGIADTEFTPEGGNGRVHLYASNGATKFQNGTTFTPATSLW
                     GDLNKLKQYDIALFSCECSQNATTKPLAAMQAIKDYADFGGRVFLSHYHNIWVDGDSA
                     DPAHAPPVWKDIATCDADGYDTGNDVIDQVKNPKGPAFAQWMQNVMGTSTPGVIPIEG
                     GRQTCTAIDESKAERWVYYQDGTAQYPQNFQFSTPNEVPEEQRCGKVVFSDMHVASGS
                     SSSSSEAFPNGCSASPMTPQEKALSFMFFDISTCLGPIGKY"
BASE COUNT         1467 a         2725 c         2787 g         1335 t
ORIGIN      
        1 gacgcgaacg tgatcgccgg tgcgggcacg ctgtccgccg agatctgcga gcaggccgac
       61 gcgctcgacg cgatcgtcgt cgcggtcggt ggtggctcgc aagccgtcgg ggcgctcgtc
      121 gcggcgcgcg cgctgcgtcc ggccgtgaag gtctacggcg tgcaggcccg agccgcgtcc
      181 gcgatccacg actcgtggca tcgcggcacg cgcctcgtca ccgagagcgc gaacacgttc
      241 gccgacgggc tggcgacgcg cgcgacgtac gacctcacgt ggccggccct gcgcgagggc
      301 ctcgcgggat tcgtcgccgt caccgaggcg cagctggcgt cggcgatccg gctctacatc
      361 cgcgcgacgc acaacctcgc cgagggcgcc ggcgcagcgg ggctcgccgg tctgctcgcg
      421 cttcgcgacg agctcgccgg caagaccgtg gcgatcgtgc tgtccggatc gaacatcgat
      481 ggcgacacgc tccgccgcgt gatgagctcg gacctgtgag gcgagccctg atcacgtgcg
      541 cgatcctcct cggctccggc gcggcgcacg ccggtccact cggcgagctg tggcggatga
      601 aggtgcgcga gtgtcccctc taccttcccg acactccggg cacgctcgcg atctcgaagg
      661 agcctgccgt cacgctctac gatcgcgcga ccggcaagcg cgtgcgcgac gtgaaggtca
      721 cgccgaaacc gaagaagagc ctctcggcga cgcggctcgg cgacacgctc gttttgcagg
      781 actggaacgt ctcgtacacg ggcgtcgatc cgaagacgga cgccgttcgc tggcgtcgcg
      841 acacgccgac caagcaaggc gagaagtact ggctcctata tccggcaggt gccgacggcg
      901 tgctggtccg tgtcgaccgc aagccgacgt tctcgctcga gctcgagcgc ttcgacggcg
      961 cgaccggcag gccgcgctgg actgcgaagc cgccgaccgc gcgtgctcag ttcgagacga
     1021 tcgtcgccgg caccaggcac acgtacctcg tcaccatgga tcgcgagccg cgcgggttca
     1081 cgatcgtggc gctcgacgag acgggcaagc catcgtggaa gatcgacgat ccgatcggcg
     1141 acctgcctca cttccatgtg gttggcgacg acctcgtggc ggtggtctcc gacgtggttc
     1201 gcgtgttcgc cggtgcgacc ggcaaggtct cgacctggaa gctgccgtgg agcccactct
     1261 acatcgtcca cggcgacacg atctatgcgc agcgcgcgag cgccgagatc ctcgcgttcg
     1321 gcggcgcgac cggcacgcag cggtgggtga cgcccatcgc cgatcactcc ggcttgctgt
     1381 tgccgctcgg cgtctcgaac ggctcgctgt acgtgaagga cggcgagcag ctgcgcgtgc
     1441 tcgacgccgc gacgggcaag ctcgcgtcga cttacggcgt cgcgggcatg gatcgcctga
     1501 aggcatggga cacggcgccg gcgatcacga tgtgcgacac tgcgacgaag acgctcgtcg
     1561 cgctcgatcc gtcggcgacg gcgaccgacc atcgcgtgac catcagcgga cggatcatct
     1621 gcaagaactg caagacgcct ccgcacgtga ccgttggtag cgtcacgacg acagccgacg
     1681 cgagtggcaa gttctcgctc gaagtgacgg cccgtggccg gctgtcgatg gtcgtgaccg
     1741 acgacgagag cctcctggcg gcgtgggttc gcaagatcac gttcgacaag gacgcgaagc
     1801 tgaagctcgg agatgttcgc gtgaaaatgc cggttccgaa cgatctagac tgacctggcg
     1861 ccgcccgcag ggtgtgtaat cgttcccgta tgaagggcat cgtcggcaag gtggtgcgcg
     1921 gcttcgtcct cgcgctcgtg tgtctcgcga tgttcgacgt cccggcgtcg tatctcgcgt
     1981 cgcggggctg gtcgccgtgg ctgtcgctcg cgttcgggct cctgacgttt ccgatcctgt
     2041 cgctcgtctg gcacgcgctg tccgagcacg cgcgcaagaa ggcgccgccg ctcaagccgg
     2101 cgacccgcgg ctgggagcgg ttcctgctgc gcagcacgtt ggtcggcgtg ctcgtgatcg
     2161 gcgcgctcgt cgccctggcc ggtggcaagc gcgtgctgag cggcttccgc aatcacgcgc
     2221 tgtggttcat ccccgacgcc acgtcgccgc tcgtcgccga ctcgaagctg ttcgacttcg
     2281 tgccgccgaa cgccgaggtg ctgatctggc tgcgcgtcac cgacgacacg cgtgcgcaac
     2341 tcggcgcggc agtgccttcg atcgagaggg tcggcaaggc gctacaggcc gaagatgtcg
     2401 tcgcggcgat cggcaaggac ggcttctgga tcggcgagca cggcgacgcc gaggtggtcg
     2461 gcgcgttcgc ggagctgttc gccgagctgc agaagctcgg caaggatcac ggcctcgatg
     2521 ccttcaccga ctcgctcgcc ggcagcgtga cgaaggcgcc cggcaatgtt cgctactggg
     2581 tgtcgcccgg ctggacgaag gccgtctcga cgggacgccc gagcgcgctg gtcgacctgt
     2641 tttcccaggc gcccgacgat gcgttcctcg tcgcggtgac gcggccggcg cccggcggca
     2701 tcgcgaaacg cgacctgccc gacgtgacga gcggcgtcgt gtacgtccgc ctgcatcgcg
     2761 gtcgagtcgt cgtcgcgatc cgcggcgact tcgcctccga caccgaggcg aagaagcgcg
     2821 ccgacgaggt gcgcgactcc accgcgagga tcgaggacca cgagtgctgg agcgtcgccg
     2881 atggtcgcgc cggcgtctcg cagaccgacc gcaccgtgac gatggagctg agcatcccgc
     2941 tcgatcgcat cgagtcggcc atgcggtgcc tcgacaagaa gtgacggcgc gcgggctcgc
     3001 gatcgcagcg gtcgtcgcgt tggcaccgac gatcgcccac gcgagccccg agctcgacgc
     3061 gctcgtgaag gacgtaccga cctgcgccgc gcgcacgtac tgcttcgcga tccgcgtgca
     3121 cgtgccgctc gacgaccggg gcgcaccgat cgccaccgcg gcgtggatcg ccggccaggt
     3181 ggccgccgcg aaccaacact tcgagaagct cgacgtcgca ttccagatcg atggcatcgg
     3241 tcccttgccg gcggccgccg cccgcgtcga ggaccgcaag gagcgcgact cgttcggcgg
     3301 ccggctgtcg cgcaaggtca tcgactggtt cgtgacgggg cagctcgacg acatcgacat
     3361 ccccggcgcg caggtcttcg gcgtcacgtg gcggcgcggc gatcgcaagt tcgtgatcgt
     3421 atcgacgcag gcccgagagc ggacgctcgc acacgagctc ggccacttct tcggcttgcc
     3481 ccattcgacg tacgcgatca gcatcatgaa caagacgccg cgcgaggagc caccacccga
     3541 ggcacgcacg ttcgccgacg aggaatacgc gaggatgaag ccgcgcgtga aggacgcggt
     3601 ccgttcacgc atcgtggcga acgtgaagcg tcgccgttga ggccgctatc cgacgagctt
     3661 catcgactcg agcatcagcg cgccgagcgc gaacgccatg cgcgcgcaga tcggcgcgac
     3721 gatgctgcga ttgccggcga cgtacatcat gccgagcccg gcaccgaaca gcccagcgcc
     3781 ggcgcgcatc gcaaagttgc ccggcgtgac gatcatctcg gcagccgatc cgatgaggat
     3841 cgccagcacg gtcacgcccg gccggccgct cgccagctcg aggacgcgct cgacgatcca
     3901 cccgcgcagc gcgagctcgg ccgcgagcga cgtgaccgcc acgtagatcg cgaccgcgaa
     3961 cagctggctc gcgttgccgc gcaccaacgt gtacatcgac cattcgacgg cccggtcggt
     4021 gacgtgctcg acgagcggcg tcccgatcgc gagcgcgagc gcgaggccga cgatgcccgc
     4081 ggtggcaccg acgatcgtcg cccgcaaccc tccgtgaaac agctcgcccc agtcacgccc
     4141 gcgcgtccac cgcgagagcg acgcgaggac gagcagcggc accgcgaccg gcacgatggc
     4201 gggcaacggc acgaaggcga ggccgacgat cgcgaggatc tcgacgatga ccaggccgcg
     4261 cagctctcga gactgggcca cgatggattt acgataccaa ggcgcgacgg ttacgaaaga
     4321 gacaccgcct tcgcgcgcgt ccttgggcgc cgaaagtcga ttgttctacg caacatgcgc
     4381 ggtcatgctg ctgtcgcgtc cagcatgagg gaacgaatcg taacgatcgg gtgtctgagc
     4441 gccctgtttc tcgggcttct cggctcggca atcgccgccg ccgcgcccac cgacgacgag
     4501 ctgaagacgc gcaaggcgga gctcgacaag aagctcgccg gtcaaggctt cacggtcgtc
     4561 gtcgaggcac cgttcgtgat cgtcggcgac gagtcggcag ccacggtgaa gggccgccgc
     4621 acgaacttca tccgctggac cgtcggcctg atcgagaagg acttcttcaa gaagcgaccc
     4681 gacaagctga tcgagatctg gctgttcaag aacatcacga cgtacaagcg cggcgcgaag
     4741 aagttcttca acgacgagcc gtcgacgccg tacggctact actcgcccga cgacaacgcg
     4801 ctcgtgatga acatcggccc gggtgccggc acgctgtcgc acgagctcgt ccatccctac
     4861 atggaagcga actttccgaa cgtgccgtcg tggttcaacg aggggctcgc gtcgttgtac
     4921 gagcagccgc gcgaacgcga gggccacatg tggggcacga cgaactggcg gttgcccggc
     4981 ctacagaagg gcatccgcga caagtcgatc cccgacgtcg cgacgctgct gaagacgacg
     5041 cgcgacggct tttacgaggc agcgtacgac tcgtacgcga tggcgcgctt cctctgccag
     5101 tacctgcagg ataagggcaa gctgcgcgac ttctacgcga agttcgcggc cgatacgaag
     5161 gacccgaccg gacaggccgt gctcgccgcg gtgctcggtc aggatctcgc ggcgttcgat
     5221 gccgcgtttc gcaagtgggc gctcgggctg cgccgctagc cgaacggacc cagaggttcg
     5281 ggcagaaagg cgccactgat gattcaacag aagtccaaca gccctcggat ttcggacttt
     5341 gaacaccaag ttgcaaagcc ccaaaggccc agaccaaggt tttctcgggg agacggctcg
     5401 agttcgagtc aaccccgcgg tgtcgaacgc ggacgttcgg gcgacctcag ccggaccaag
     5461 acgtcatcga cgcatgttcg acggcgtctc gcgttcgagc gcaaacgaga cccgatcgaa
     5521 agatgagtgt ctgggctgtc ggactgctcg agctggttcg ggatctcgcc gctagagagc
     5581 cgatccacta acgcggcttg gggatcgacg ccggtctgag gcggtgatcc actcggtcca
     5641 tgtattggtt gccgagtcac agcaaggccg agaagcagat cgcagggagc cttcgtccag
     5701 cgagcgcatt ctttcgattc ctctgggaaa cgcgcgacga gctgttcagt cgcgagttcc
     5761 aagacgagtt ggtgatgagc taccaaccgc gggggcagga accatgtgcg ccggctctgc
     5821 tggcgatggt gatgcttctg cagcgctata cggggcttag cgatgcggac gctgtcgacg
     5881 cggcagagaa cgaccggcga tggcagctcg ttcttgggtg tctcggcgca gaaaaggcgc
     5941 ccttcggaca gggcactctg gtccggttcc gaacccgtgc gatcgcgaac gacctcgaca
     6001 agaagctcgt cgagaggacg gtcgagcttg cgaggaagac cagccgcttt ggctggaaga
     6061 agctccgcgt ggcactcgac tcgtctccgt tgcatggtgc cggacgggtc gaggacacgt
     6121 ggaacctaat cgggagggcg atggctaagg tcgttcacgc tgtcagcctc gcgctcgata
     6181 tcgaggagga gaccatcatc aagagtgccg gcctaacggt gctttccggc gacagcatca
     6241 agggagccct tgatatcgac tggaacgatg acgatgcaca ggtcgccgcg cttcagaagc
     6301 tgctccacca ggtggacacg ctggaacaat gggtcgcgaa acgggcagct aagcaagccg
     6361 aggtaccgcc gctcaaagag tcgttggacc tgcttcgcaa ggtcgttgac caagacatcg
     6421 agcccgaccc gtcaggcagc ggacggcgca tcaaacaaga ggttgcgaag gaacgggtga
     6481 tctcgatctc cgatccggag atgcggcatg gtcgaaagag caagacgaag ctgttcaacg
     6541 gtttcaagcg tcacatcgcg atcgcgaaca acttgatcct cgcgacggtg gtcgtcccgg
     6601 ccaatatcca agagcacgag gccacagcgc ctttgctcaa agttgtcgag gaatatggcg
     6661 acatccacac gctcgacatc gaccgcggct acctgccaag tccccgagtg gccgagctcc
     6721 atcgagacgg cactgtcatc cattcgcggc cgtgggcacc gacaaacaag ggactgttca
     6781 cgaaggatga cttttcgatc aacattcgtc gtcgagaagt cacgtgtcca aacggcaaga
     6841 tagcgaaggc tgtagacagc ggaaaagcag cattcgccgt cgacgattgc gcgaagtgca
     6901 agctgaaacc gagctgcacc acgtcctcgc atcgaacggt aaccctgcat cccgctgaag
     6961 acctcttggt caagctgcga cggagcaacg cctccaagca cggacgtgcg gagctgcgtg
     7021 aacgtgtggc agtcgagcac cacctcgctc gcatcggcag catccagggc gacacggcga
     7081 ggtatgcggg tgccaggaag aacgagctgg acctcaatcg gtccgcagcc atcgccaacc
     7141 tgcacgaggt cgcccgacgc cgtgccgctt agcggttcgg ctctctagta cttgccgatg
     7201 gggccgaggc aggtcgagat gtcgaagaac atgaacgaca gcgccttctc ctgcggcgtc
     7261 atcggggagg ccgagcagcc gttcgggaac gcttcgctcg agctcgacga gctacccgac
     7321 gcgacgtgca tgtcggagaa cacgaccttg ccgcagcgct gctcctcggg gacctcgttc
     7381 ggcgtcgaga actggaagtt ctgcggatac tgcgccgtgc cgtcctggta gtagacccag
     7441 cgctcggcct tgctctcgtc gatcgcggtg cacgtctggc ggccgccctc gatcgggatc
     7501 acgccgggcg tcgacgtgcc catcacgttc tgcatccact gtgcgaacgc cggacccttg
     7561 ggattcttca cctggtcgat gacgtcgtta ccggtgtcgt agccatcggc gtcgcacgtc
     7621 gcgatgtcct tccagaccgg cggcgcgtgc gcggggtcag ccgagtctcc gtcgacccag
     7681 atgttgtgat agtgcgagag gaacacgcga ccgccgaagt cggcgtagtc cttgatcgcc
     7741 tgcatcgcgg cgagcggctt ggtcgtcgcg ttctggctgc actcgcacga gaacagcgcg
     7801 atgtcgtact gcttcagctt gttcaggtca ccccacagcg acgtggccgg cgtgaacgtc
     7861 gtgccattct ggaacttcgt cgcgccgttg ctcgcgtaga ggtgcacgcg gccgttgccg
     7921 ccctcgggcg tgaactcggt gtcggcgata cccagcttgc gaacgaggca ctcgagtgcg
     7981 tcgcagctac cggtcgcgat cgccattttc gggatgtcgc cttcctggcg gctcgtcggt
     8041 agcgacgtca gctcgggagg cagcgggttg tcctggcatt cgttcaccat cgggatcgcg
     8101 acgcgcttgc gccacttgcc gacggtcacg atcagcggga tgtcgcgacc ggccgggacg
     8161 tccgacatcg tgaagcgacc cgcttcgtcc gacttcgcgg acgtgatcgg ctcgcccgga
     8221 atctgctggt cgcagcggcc gcactgggcg ccctccatga acggacccgg gtcggcgttc
     8281 ggcacgtaga tggtgacgcc gtagagaggc aacg
//
LOCUS       DATLBZ010000533        14004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7795271, whole genome shotgun sequence.
ACCESSION   DATLBZ010000533 DATLBZ010000000
VERSION     DATLBZ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14004
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7795271"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..659)
                     /locus_tag="VMZ53_26735"
     CDS             complement(105..659)
                     /locus_tag="VMZ53_26735"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUS32136.1"
                     /translation="MKRVLLVAAVLAATTATASAGGFVGLGIGGEAGVSSDSSLAADG
                     RSGRLEVGYSFGRLSVEGLVSRFDLYNTTDYAGYTDTSLGIAATFGVPLSAGFGVYGR
                     GGLQHTTLSVDESASRADRHYNGSGLLFGGGFDYHLPLAATNLALFVDYTITTSSLTI
                     DELPGIEAYQFTTRNWMLGAKLGF"
     gene            complement(824..3004)
                     /locus_tag="VMZ53_26740"
     CDS             complement(824..3004)
                     /locus_tag="VMZ53_26740"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HUS32137.1"
                     /translation="MGSPDVDLILATRHRRRVRGAIEIGVGLALIVAGIIITLLTYNK
                     TAKSGGMYVLAYGPVVLGFFGIVRGTIRMLRSEHGLMMPSVKHGTRYDLTIGVGFKSF
                     VAWRYLMARHHRVSRAVLFVFFLGLFITVVSTTIQLTLATKANPLKLDPLQMQNFARY
                     VSAGLALGLVLLYFTALIGALRFIFSFFTTVPLVGVWIGTGALVCVLSVMSGFESDLR
                     QKILGSNAHIQVTAEEGEMKDWREIKKKIDAVPGVIASTPYAVSEVVIASNSNGMNVI
                     IKGIDPETVGSVTKLVSSIQDPDKAAVMKRLDPIVDDNYNLEVPGTGSGAGSGSGSGS
                     AGAGSGVLDPPPDDMPGTGDPIDFSQPDPALRGWDEPPALDDIDHPPPPLDGSNAIAK
                     GSDQQPPMPELVPGPHIATPVVDPPPDDLVTSEEPPTDYSGREDEDDDTIHVVDVPLD
                     QQPSLSRRTQSLPGILVGKELVKQTHLYTGEEVRVVSPLSDPSNPDATGTPIPFNRDY
                     RVAGEFFTGMYEYDLKYVYVTLDSLQDFLDRGDAVDGIEVRIGSPDDTDAYVAKLQRT
                     LGKEYRIQDWKELNRSLFSALKLEKIAMFLVLGIVILVASFSIVGNLIMVVVEKGREI
                     ALLKTLGASDASLMLVFAIQGVLIGLIGTALGVGTGLLACYLGKTFGIPLNPDVYYID
                     KMPVHVDPQSVLITAFAGVMISMAATFYPAFVAARVRPAAGMRH"
     gene            complement(3008..4663)
                     /locus_tag="VMZ53_26745"
     CDS             complement(3008..4663)
                     /locus_tag="VMZ53_26745"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HUS32138.1"
                     /translation="MSEEQSTLERIISERRAKAAALRTHGSDPYRNDIGPSVGLGAVR
                     AKYGPTKPETPTGDPIDGETLRVSGRVMGKRVVSKKLSFAPIRDASGDIQLFLSLQHL
                     DADDHAKILPEVDTGDIVVAEGPAVWTKTGELSILVKRLWLVTKSLRPLPDKWHGLTD
                     VEARYRQRYVDLAVSPDVRDVFRKRSAIVRGIRRYLDERNFLEVETPMMHPIIGGAAA
                     RPFTTHHNALDMKLYMRIAPELYLKRLVVGGFERVYEINRNFRNEGLSRQHNPEFTML
                     EFYQAYATYTDLMDLTEKLVGELTQEVNGATKVTWDGIEVELAAPWRRLSIHDAVRDL
                     GNIAEASRVFADPAFAAEVAIAHGISPGEIARALLEGVPKGTFGQPAEEVVAGFKDPS
                     SRPSLAANMIERYPSEVERRVRAGHIGYMIFEHTAESKLVQPTFLTQFPLAVSPLARK
                     NDSDPAFCDRFELFVCGKEIANGFSELNDPDDQRSRFQAQLAAKDVGAAETMDYDEDY
                     CRALEIGMPPTAGEGIGIDRLTMLLTGQSSIRDVILFPLMRNE"
     gene            4700..6136
                     /locus_tag="VMZ53_26750"
     CDS             4700..6136
                     /locus_tag="VMZ53_26750"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HUS32139.1"
                     /translation="MRRLALFALLVLAACKGDPKGKVEATGSAGSGSDHFTEVIHGEP
                     CKNAQQKGPIAWIEDDYASALACAKQKKLPLVLDLWAPWCHTCIAMQTTVFMDPSMEP
                     RMKKFVFAALDTDREENAAAVGKFSTSAMPTFYVIAPDETVLARFVGAATLDQFQEFL
                     DSGLRSQTGSLAEADAKFLAGQRAMAKKDYEAADKELSAALELGPQAWLARPEVIYYL
                     QMVRTKRGDHAGCMKLSDEQMDAVGQTAIATNFWQTAIDCATAEAKTDAAKSKAVIAR
                     AIAKLQKTLDDTAAPLSPDDRAEALGYLRDALDAQGKKAEAKAAAEKARTFIDEQWTK
                     AATPFARMAWAWPRAEVYGYLGRPLDLVADYEKLATDLPKEYEPRARLGWLYLKAGNA
                     GEAAKWTDLALARVYGPRKGRLLNQRAEIAALTGEKSVEKMYREQAVKLWETLPEGQR
                     NADALAKAKAALAAVDAPAAGSGSAAKK"
     gene            6209..7201
                     /locus_tag="VMZ53_26755"
     CDS             6209..7201
                     /locus_tag="VMZ53_26755"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HUS32140.1"
                     /translation="MRPRVLGIVALVVAVIGLGASIASLVDYLSAKPTFCMESGCATV
                     RASEWSHPLGIPLPIFGIVFFATAIALSFIDRPRMRLVWSLGGAAVALGLIALQAFSI
                     HAWCKLCLVADPSALLYAAAVVAGGATLKPTWRASAVAPGVLLVVLALGLWTHQEAAP
                     AQPPPDVVLKEQKRGVVTIVEFVDFECPFCRALDKKLRVAVDRTKKPVRFVRKMVPLP
                     AHEHSVPAAMAYCAAEAQGVGDEMARRLFELPPAELTPAGCEKVAVELGCDVMKYRET
                     FASAELRARIEADMADARAAQLEGFPTIFIGTQKFEGSDHSSEKLLAAIERAGE"
     gene            7296..7589
                     /locus_tag="VMZ53_26760"
     CDS             7296..7589
                     /locus_tag="VMZ53_26760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32141.1"
                     /translation="MPTLATIDLETLATVTGGRWTLPRSPMSPGSGPMTLMGLLDKPG
                     RTLDLIRQGLGMPETGAPGSTYVPATANPDGSVNQGRFETPRDPNVPEVPMSQ"
     gene            complement(7594..8616)
                     /gene="prfB"
                     /locus_tag="VMZ53_26765"
     CDS             complement(7594..8616)
                     /gene="prfB"
                     /locus_tag="VMZ53_26765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HUS32142.1"
                     /translation="MKIEELDSIAARPNFWDDQKKAQSVLRDKKHLEQVVTSWDAQSR
                     ALSDAEVLLDLADEASDEDSAKEAAAQVLGIQKALDDLEFKRMLSGELDGGGAIVQIT
                     SGAGGVDASDWAQMLMRLMIRYCDRKSWKVDVLEETPAEEAGIKQATFTVAGDYAYGL
                     LKAENGVHRLVRVSPFDANGRRQTSFAAVTVTADIDDDIEVKYDEADLRIDTYRAGGA
                     GGQHVNKTDSAVRITHMPTGIVVQCQNERSQHANKDKAFKLLRAKLYDYEVAKREAKA
                     AEDAKARLKIEWGSQIRSYVLFPYQQVNDHRTEVKTSDVQGVLDGDLDELIRTYLLQQ
                     ANGKKN"
     gene            complement(8733..10127)
                     /gene="purF"
                     /locus_tag="VMZ53_26770"
     CDS             complement(8733..10127)
                     /gene="purF"
                     /locus_tag="VMZ53_26770"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HUS32143.1"
                     /translation="MCGVFGIHGHDEAANIAYLGMHALQHRGQESAGLVAADEGRLRR
                     HVSMGLVSDAFNRDVLAKLPGSTAIGHVRYSTAGSSELRNAQPFLFEYAGGSIAIAHN
                     GNLVNAAELRVELEAAGSLFQTSSDTEVIVHLMAKAREPDVVGKLRAALSRVRGAYSL
                     IVLATDGPHTKMIGVRDPNGFRPLVIGRLKDAYVLSSETCSFDLIEGDLLREVEPGEI
                     VVIENGGMVSHRLDSQPQTFCVFEHVYFARPDSLVNGKSVYRAREKMGMRLAQESPVE
                     ADVVIAVPDSGVPAAIGYSRQSGIPFEMGLIRSHYVGRTFIEPQDSIRHFGVRLKLSP
                     VRSIVDGKRIVVVDDSLVRGTTSRKIVKMLRAAGAKEVHLRISAPPTTHPCFYGIDTP
                     NRSELVAASHSVDEINKYVTSDTLVYLSHEGMMGAVSGDPSGSRYCSACFTGKYPVPL
                     GQQNADLVQLRRVR"
     gene            10208..11140
                     /locus_tag="VMZ53_26775"
     CDS             10208..11140
                     /locus_tag="VMZ53_26775"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS32144.1"
                     /translation="MPRLLWRDAQGIEGSLDLTSAEIKVGRAMDCAIRTDDAMVSRHH
                     SRIFWGGGGYVLEDLSSANGVYFQEQRVQSHLFKHGDAVRCGSLWLRFVDQAQIGATP
                     AAGPGTPAPMMQHPGMGAIAPAGPTGHVAAINPGGMSASMPAQQPGAAMAALGVAPAN
                     QESDAEIRVLRRRVEQLQTELRMYRSQKFKEDNVRRMEDLENDLSMMEIERDNLKTRL
                     DKAEQSLKLEAGSIKVKRALEIAAMTAETAASLNDLLSSLRIEVMAAEGEFDQYAHSI
                     PRASFELIRQSLKDAASHCDDARELLRKLREVAE"
     gene            11480..11848
                     /locus_tag="VMZ53_26780"
     CDS             11480..11848
                     /locus_tag="VMZ53_26780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32145.1"
                     /translation="MAALPLAQSSWHGPEVASTLAVAAVALLAGQRWAISIVVLAELL
                     LLPTIWPRAFLGGVELWPGRIAALTSLAMIVPGLLSVRRAAAALVLITGWRRTQRTCR
                     HLQYAFGVLFLIALLLPLIA"
     gene            complement(11854..13329)
                     /locus_tag="VMZ53_26785"
     CDS             complement(11854..13329)
                     /locus_tag="VMZ53_26785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32146.1"
                     /translation="MMTRVTCLLLALAGCSFYVDKDITQCDVDSDCESFGNHPYCQEG
                     VCVDSHLGPPGCFFGEPTTQEEFANRCTTSQTFQYDNCAKLGLCDQGALDAAWTTTAA
                     PQSLGTVPPPVNTQPTPTVNCVDVNPNMVYITGSTNLPPLVKAVQPLLYAGNPSYVAV
                     FAPQTSCRGAGSILDPDATKHLIKNVTNNYAFYYDQQGKQTFCLLDPAGTVVDVGESD
                     VNASACGFSAVANTADYVGPVLTIDLVVPSSSSQTTISAEAAHLVFGAGGASGKVTPW
                     TDPHYYFTRSSGTGTTQLVSRAIGVDATMWWGIDRLSAGNLVASMEAIDPTVSESVIG
                     VLSADYADKAKANLRLLAFQQKGQKYAYLPDSTPQVSDKANVRDGHYPIWGAIHFFAS
                     TANGVPSQAASAFITQFSVPKLKEELVSAIIDAGFVPSCAMKVSRTVDVGPLSPYTPP
                     FGCGCYFDNKVNGATACKKCTTASDCTSAAPACNYGFCEKQ"
     gene            complement(13341..>14004)
                     /locus_tag="VMZ53_26790"
     CDS             complement(13341..>14004)
                     /locus_tag="VMZ53_26790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32147.1"
                     /translation="PDVPAERQKEVNEEIDRLKGRVGSVTIQTAPGAEILIDDVAVGF
                     APLPEPVVVSAGQHRIVVHVQGRDPLQRVYDVAGRQELTVSMGQDLAPSAPVVKDTAP
                     VTAVTKQDEAPSKTPIYVTWTITGAFAAGAITFAWFARSDSHKLETLRNTYPVTPEQL
                     QDQKDKAVRDAAIADGLMAATLVTAGIATYFTLTRRDSKKAEKSVQLRVGPGAIGIAG
                     AF"
BASE COUNT         2422 a         4416 c         4659 g         2507 t
ORIGIN      
        1 aatcctaaag ggcgcaggac cttacttacg gcacctgcaa gtgtcaacgc aggttgggac
       61 ctatgccgag ccctttaggt tgcccggaga tcgggagtta aacgctagaa gccgagcttc
      121 gcgccgagca tccagttccg cgtcgtgaac tgataggcct caatgcccgg aagctcgtcg
      181 atcgtgaggc tgctcgtcgt aatcgtgtag tcgacgaaca gagccagatt cgttgccgca
      241 agcggcaggt ggtaatcgaa cccgccgccg aacaggaggc cactgccgtt gtagtggcgg
      301 tcagcgcgcg aagcgctttc atcgacgctg agcgtggtgt gctgcagacc accgcggcca
      361 tagacgccaa agccggcact gagcgggacg ccgaaggtcg cggcgattcc gagcgaggtg
      421 tccgtatagc cggcgtaatc cgtcgtgttg tagaggtcga aacggctcac gagaccttca
      481 acggagaggc ggccgaagga gtaaccaact tcgagccgac cgctgcggcc gtctgccgcg
      541 agactgctgt ccgagctaac gccggcctcg cctccgatgc cgagcccgac gaagccgccg
      601 gcagaagccg tggcggtggt ggcggcgagg acggcggcga cgagcaggac gcgcttcatg
      661 cgcgcgatcc atgagcattc gccatgccat ctataagtcc tcgaaagacc gcacccaggt
      721 catggaaacc tgtcaccgtt ccttcactac ctgggctcac ctgacgcagc agcgattgcg
      781 gcgactttct cgacgaaagc caaaccgcga tcggattgtc gcgttagtgg cgcatgccgg
      841 cagccgggcg aacgcgcgca gcgacgaagg ctgggtaaaa cgtggcagcc atcgagatca
      901 tcacgcccgc gaacgcggtg atgagcaccg actgcgggtc gacgtgaacc ggcatcttgt
      961 cgatgtagta aacgtcgggg ttcagcggga taccgaaggt cttgccgagg taacaggcga
     1021 gcaggcccgt gcccacgccg agtgcagtgc cgatgaggcc gatcagcacg ccttggatcg
     1081 cgaacacgag catcagcgag gcatcgctcg cgccgagcgt cttaagcagc gcgatctcgc
     1141 ggcctttctc gacgacgacc atgatgaggt tgccgacgat cgagaagctc gcgacgagga
     1201 tcacgatgcc gagcacgagg aacatcgcga tcttttcgag cttgagtgcc gagaagagcg
     1261 agcggttgag ctccttccag tcttggattc ggtactcctt gccgagcgtg cgttgcagct
     1321 tcgcgacgta cgcgtcggtg tcgtctgggc tgccgatgcg gacttcgatg ccgtcgaccg
     1381 cgtcgccgcg atcgaggaag tcttgcagtg agtcgagcgt cacgtagacg tacttgaggt
     1441 cgtactcgta catgccggtg aagaactcgc cggcgacgcg gtagtcgcgg ttgaacggga
     1501 tcggcgtgcc cgtcgcgtcg gggttcgacg gatcggagag cggcgacacg acgcgcactt
     1561 cttcgccggt gtagaggtgc gtctgtttga cgagctcttt gccgacgagg attccaggca
     1621 gcgactgcgt gcggcgtgag agcgagggct gctgatcgag aggcacgtcg acgacgtgga
     1681 tggtgtcgtc gtcttcgtct tcgcggcccg agtagtcggt gggcggctct tcgctcgtca
     1741 cgagatcgtc gggcggtgga tcgacgacgg gcgtcgcgat gtgcggaccg gggacgagct
     1801 cgggcatcgg cggctgctga tcgctgcctt tcgcgatcgc gttcgaaccg tcgagaggtg
     1861 gcggcggatg atcgatgtcg tcgagcgccg gcggctcgtc ccagccacgc agcgcgggat
     1921 cgggctggga gaaatcgatc ggatcgccag tgcccggcat gtcgtcgggc ggtggatcga
     1981 ggacgccgga gcccgcgccg gcggagcccg agccggagcc cgaaccagcg cccgagcctg
     2041 tgccgggaac ctcgaggttg tagttgtcgt cgacgatcgg atcgagccgc ttcatcaccg
     2101 cagccttgtc cggatcctgg atcgagctca cgagcttcgt caccgagcca acggtctcgg
     2161 gatcgatgcc tttgatgatg acgttcatgc cgttgctgtt cgaggcgatg acgacctcgc
     2221 tgactgcgta cggcgtcgac gcgatgacac cggggacggc gtcgatcttc tttttgatct
     2281 cgcgccagtc cttcatctcg ccttcttcgg ccgtgacttg aatgtgcgcg ttcgagccga
     2341 ggatcttttg gcgaaggtcg ctctcgaagc cgctcatcac ggagagcacg cacacgagcg
     2401 cgcccgtacc gatccaaacg ccgacgagcg gcaccgtcgt gaagaacgag aagatgaagc
     2461 gcagcgcgcc gatcagcgcg gtgaagtaga gcagcacgag accgagcgcg agccccgccg
     2521 agacgtagcg cgcgaagttc tgcatctgca gcggatcgag cttgagcggg ttggcctttg
     2581 tcgcgagcgt gagctggatc gtcgtcgaga caaccgtgat gaacaggccg aggaagaaga
     2641 cgaacaggac cgcgcgcgag acgcggtgat ggcgagccat caggtagcgc cacgcgacga
     2701 acgacttgaa gccgacgccg atggtgaggt cgtagcgcgt gccgtgcttg accgacggca
     2761 tcatcagccc gtgctcggac cgcagcatcc ggatcgtgcc gcgcacgatg ccaaagaagc
     2821 cgagcacgac gggcccgtac gcgagcacgt acatgccgcc tgacttcgcg gtcttgttgt
     2881 acgtgaggag cgtgatgatg atgcccgcga cgatcagcgc gagaccaacg ccgatctcga
     2941 tcgcgccgcg aacacgccgg cgatggcgcg ttgcgaggat gaggtcgacg tcagggctgc
     3001 ccatggatca ctcgttccgc atcagcggga acaggatgac gtcgcggatc gacgactgcc
     3061 cggtaagcag catcgtcagg cgatcgatgc cgatgccctc gcctgccgtc ggcggcatgc
     3121 cgatttcgag cgcgcggcag tagtcctcgt cgtagtccat cgtctcggcc gcgccgacgt
     3181 ctttcgcagc gagctgcgct tggaagcgcg agcgctggtc gtcgggatcg tttagctccg
     3241 agaagccgtt cgcgatctct ttgccgcaga cgaacagctc gaaccgatcg cagaacgccg
     3301 gatcgctgtc gttcttgcgc gcgagcggcg agacggcgag cgggaactgc gtcaggaacg
     3361 tcggctgcac gagcttggac tcggcggtgt gctcgaagat catgtagccg atatgacccg
     3421 cgcgcacgcg tcgctccact tcggagggat agcgctcgat catgttcgct gccagcgaag
     3481 gtcgcgagct cggatccttg aagccagcga caacttcctc ggcgggctgg ccgaacgtgc
     3541 ccttcggaac gccttcgagc agcgcgcgcg cgatttcgcc gggcgagatg ccatgcgcga
     3601 ttgccacttc ggcagcaaat gccggatcgg caaagacgcg tgacgcttcg gcgatgttgc
     3661 cgaggtcgcg aaccgcatcg tgaatcgaga gccgacgcca cggcgcagcg agctcgacct
     3721 cgatgccatc ccacgtgacc ttcgttgcac cgttgacctc ttgcgtgagc tcaccgacga
     3781 gcttctcggt gagatccatg agatcggtgt acgtcgcgta cgcttgatag aactcgagca
     3841 tcgtgaactc ggggttgtgc tggcgggaca acccttcgtt gcggaagttg cgattgattt
     3901 cgtacacgcg ctcgaatccg ccaacgacca ggcgcttcag gtagagctct ggcgcgatgc
     3961 gcatgtagag cttcatgtcg agcgcgttgt ggtgcgtcgt gaacgggcgc gccgctgcac
     4021 caccgatgat cggatgcatc atgggcgtct cgacttcgag gaagttccgc tcgtcgaggt
     4081 aacggcgaat gccgcgcacg atcgccgacc gcttgcggaa cacgtcgcgc acgtcaggcg
     4141 agaccgcgag atcgacgtag cgctggcgat agcgtgcttc gacgtccgtg aggccgtgcc
     4201 acttgtcggg gagcggtcgc agcgacttgg tgacgagcca cagccgcttc acgaggatcg
     4261 agagctcgcc ggtcttcgtc cagacggcag gcccttcggc gacgacgatg tccccggtat
     4321 cgacctcggg caggatcttc gcgtgatcgt cggcgtcgag atgttggagg ctgaggaaga
     4381 gctggatgtc gccgctcgcg tcgcgaatcg gtgcgaacga cagcttcttg ctgacgacgc
     4441 gcttgcccat cacgcggccc gacacgcgca gcgtctcgcc gtcgatcgga tcgccggtcg
     4501 gcgtctcggg cttcgtcggt ccgtacttgg cgcgcactgc accgaggcca acgctcggcc
     4561 cgatatcgtt gcggtatgga tccgatccgt gcgtgcgcaa cgcagcggcc tttgcgcgcc
     4621 gctcactgat gatgcgctcg agcgtggact gctcctctga catggcggcg gaccttacca
     4681 cctgtgctat cacgcggaca tgcgtcgttt ggccctgttc gcgctgttgg ttctcgccgc
     4741 ctgcaagggc gatcccaagg gcaaggtcga ggcgaccggc tccgcgggct cgggcagcga
     4801 ccacttcacc gaggtgatcc acggcgagcc gtgcaagaac gcgcagcaga agggcccgat
     4861 cgcatggatc gaggacgact acgcctctgc cctcgcctgc gccaagcaaa agaagctccc
     4921 gctcgtgctc gatctatggg cgccgtggtg ccacacgtgc atcgccatgc agacgacggt
     4981 gttcatggat ccctccatgg agccgcgcat gaagaagttc gtgttcgcgg cgctcgatac
     5041 cgatcgcgaa gagaacgccg ccgcggtcgg caagttctcg accagcgcca tgccgacgtt
     5101 ctacgtgatc gcgccggacg agacggtgct cgcgcgcttt gtcggcgcgg cgacgctcga
     5161 tcagttccag gagtttctcg attcaggtct gcgctcgcag acgggctcgc tcgccgaagc
     5221 cgacgcgaaa tttctcgcgg ggcaacgcgc gatggcgaag aaggactacg aggcggccga
     5281 caaggagctg tccgcggcgc tcgagctcgg gccgcaagcg tggctcgctc gccccgaggt
     5341 catctactac ctgcagatgg ttcgcacgaa acgcggcgat cacgccgggt gcatgaagct
     5401 ctccgacgaa cagatggacg cggtcggcca aaccgcgatc gcgacgaact tctggcaaac
     5461 ggcgatcgat tgcgcgacgg ccgaagcgaa gacggacgcg gcaaaatcaa aggccgtgat
     5521 cgcgcgcgcg atcgcaaagc tgcagaagac actcgacgac acggccgctc cactctctcc
     5581 tgacgatcgc gccgaagcgc tcggctacct acgcgacgcg ctcgatgcac aaggcaagaa
     5641 agccgaggcg aaggcggccg cggagaaggc gcgcacgttc atcgacgagc aatggacaaa
     5701 ggctgcgacg ccgtttgcgc gcatggcgtg ggcgtggccg cgcgccgaag tctacgggta
     5761 cctcgggcga ccgctcgacc tcgtcgccga ttacgagaag ctcgccaccg atttgccgaa
     5821 ggagtacgag cctcgcgcgc gactcggctg gctgtatctc aaagcgggca acgcagggga
     5881 agccgcgaag tggacagacc tcgcgctcgc acgcgtgtac gggccgcgca aaggccgcct
     5941 gctcaaccag cgcgccgaaa tcgcggcgct caccggcgag aagtcggtcg agaagatgta
     6001 tcgcgaacaa gcggtgaagc tgtgggagac gttgcccgaa ggtcagcgca acgccgacgc
     6061 gctcgccaaa gcgaaagctg cgctcgccgc ggtcgacgca cctgccgccg gttcgggctc
     6121 cgcggcaaaa aagtgacgcg ctcaccggtc tgaagcaata agcgtcacag ctggaattcg
     6181 ccgcggggct tgttaggttc gggaggcaat gcgcccgcgc gttctcggca tcgttgccct
     6241 cgtcgtcgcc gtgatcggcc tcggcgcgag catcgcgagc ctcgttgact acctctcggc
     6301 gaagccgacg ttctgcatgg agagtggctg cgcgacggtg cgtgcgtcgg agtggtcaca
     6361 tccactcggc attccgctgc cgatcttcgg catcgtgttc ttcgcgacgg cgatcgcgct
     6421 ctcgttcatc gatcgtcctc ggatgcggct cgtgtggtcg ctcggtggcg ccgcggtagc
     6481 gctgggcctc atcgcgctgc aggcattctc gatccacgcg tggtgcaagc tgtgcctcgt
     6541 cgcggacccg tcggcgctct tgtacgcggc ggctgtcgtc gcgggcggcg caacgctgaa
     6601 gccgacgtgg cgcgcatcgg ccgttgcgcc gggagtcctg ctcgtcgtgc tcgcgctcgg
     6661 cttgtggacg catcaagagg ccgcacccgc gcagccgccg ccggacgtcg tgctcaagga
     6721 gcagaagcgc ggcgtcgtga cgatcgtcga gttcgtcgac ttcgagtgtc cgttctgccg
     6781 cgcactcgac aagaagctgc gcgtcgccgt cgatcgcacg aagaagcccg tgcgcttcgt
     6841 ccgcaagatg gtgccgctac ccgcgcacga gcactcggtg ccagcggcca tggcgtattg
     6901 cgcggcggag gcgcaggggg ttggcgacga gatggcgcgc cggttgttcg agctaccgcc
     6961 ggctgagctg acgcccgcgg gttgcgagaa ggtcgccgtc gagctcggct gcgacgtgat
     7021 gaagtatcgc gagacgttcg cgagcgccga gctgcgcgca cgcatcgagg cggacatggc
     7081 ggatgcacgc gccgcccagc tcgaaggctt cccgacgatc ttcatcggca cgcagaagtt
     7141 cgagggcagc gaccactcga gcgagaagct gctcgcggcg atcgaacgcg caggcgagta
     7201 gacacacctg tctacggcgg aacgcattgc ggtcccgcaa ctggtgcaac cgcgctcggt
     7261 tacgcgtggc ccgacgtttg aactacgtgc acgcgatgcc taccctcgca acgatcgacc
     7321 tcgagaccct cgccaccgtg accggaggtc gctggacctt gccgaggagc ccgatgtctc
     7381 cgggcagtgg tccgatgacg ctgatgggtc tcctcgacaa gccgggccga acgctcgacc
     7441 tgatccgcca gggcctcggc atgccagaga ccggcgcgcc aggcagcacc tacgttccgg
     7501 cgacggcgaa cccagatggc tccgtcaacc agggtcggtt cgagacgccg cgtgatccga
     7561 acgtccctga agtcccgatg agccagtagc cggctagttc ttcttgccgt tcgcctgctg
     7621 cagcaagtac gtgcgaatga gctcgtcgag atcgccgtcg agcacgccct gcacgtcgct
     7681 cgtcttcacc tcggtgcggt gatcgttgac ctgttgatac gggaacagca cgtagctgcg
     7741 aatctgcgag ccccactcga tcttcaagcg cgccttcgca tcttcggcgg ccttcgcttc
     7801 gcgcttcgcg acttcgtagt cgtagagctt cgcgcggagc agcttgaacg ccttgtcctt
     7861 gttcgcgtgc tggctgcgct cgttctgaca ctgcacgacg atgccggttg gcatgtgcgt
     7921 gatgcgaacg gcggagtccg tcttgttgac gtgctgacca cccgcaccac ccgcgcgata
     7981 ggtgtcgatg cgcagatccg cctcgtcgta tttgacctcg atgtcgtcat cgatgtcggc
     8041 ggtcacggtg actgctgcaa acgaggtctg ccggcgcccg ttcgcgtcga acggtgacac
     8101 gcgcacgagc cgatgcacgc cgttctcggc cttgagcaag ccgtacgcgt aatcgcccgc
     8161 gacggtgaac gtcgcctgct tgatgcccgc ctcttcagcc ggcgtctctt cgaggacgtc
     8221 gaccttccag ctcttgcgat cgcagtagcg aatcatcaag cgcatgagca tctgtgccca
     8281 atcgctggca tcgacgccgc cggcaccgct cgtgatctgg acgatcgcgc caccaccgtc
     8341 gagctcgccc gacaacatcc gcttgaactc gagatcgtcg agtgccttct gaatgccgag
     8401 cacctgcgcg gcggcttcct tcgcggagtc ctcgtcggat gcttcatccg cgaggtcgag
     8461 caacacctcg gcatccgaga gagcgcgtga ctgcgcatcc cagctcgtga cgacctgctc
     8521 gaggtgcttc ttgtcgcgca gcacgctctg cgccttcttt tggtcgtccc agaaattcgg
     8581 gcgcgccgcg atcgaatcga gctcttcgat cttcaggcgt ttgtggtcga cgtcaaagca
     8641 acctccggag ctcggtcacc ttgccggaga gctccttcag ctgcgcgcgg actgcgcgca
     8701 catccgtacc ttcgatcgcc atggcccgcg ttttaccgga cgcggcgcag ctggacaagg
     8761 tcggcgttct gctggccgag cgggaccggg tacttgcccg tgaagcatgc cgagcagtag
     8821 cggcttcccg acggatcgcc ggagaccgcc cccatcatgc cttcgtgcga gaggtacacg
     8881 agcgtgtcac tcgtgacgta cttgttgatc tcgtcgaccg agtgcgaggc cgcgacgagc
     8941 tcgctgcggt tcggcgtgtc gatgccgtag aagcacggat gcgtcgtcgg cggagcgctg
     9001 atgcggaggt ggacctcttt ggcgcccgcg gcgcgcagca tcttgacgat cttgcgcgac
     9061 gtggtgccgc gcacgagcga gtcgtcgacc acgacgatgc gcttgccatc gacgatcgag
     9121 cgaacgggcg agagcttgag gcgcacgccg aagtggcgga tggaatcctg cggctcgatg
     9181 aacgtgcggc cgacgtagtg gctgcggatg aggcccatct cgaacgggat gccgctctgg
     9241 cgcgagtaac cgatcgcggc ggggacgccg gagtccggga ccgcgatgac gacgtcggct
     9301 tcgactggag attcttgcgc gaggcgcatg cccatcttct cgcgcgcgcg atagacgctc
     9361 ttaccgttga cgagggaatc ggggcgcgcg aagtagacgt gctcgaagac gcagaacgtt
     9421 tgcggctgtg aatcgaggcg gtgcgagacc atgccgccgt tctcgatcac gacgatctcg
     9481 ccgggctcga cttcgcgcag caggtcgcct tcgatgagat cgaacgagca cgtctcgctc
     9541 gaaaggacgt acgcgtcctt gaggcggccg atgacgagcg ggcggaagcc gttcggatcg
     9601 cgcacgccga tcatcttggt gtgcggcccg tcggtcgcga ggacgatgag cgagtacgcg
     9661 ccgcggaccc gcgagagcgc cgcgcgaagc ttgccgacca cgtctggctc acgcgctttc
     9721 gccatgaggt ggacgatcac ctcggtgtca ctcgacgttt ggaacagcga gcccgcagct
     9781 tccagctcga cgcgcagctc tgcggcgttg acgaggttgc cgttgtgggc gatggcgatc
     9841 gagccgcccg cgtattcgaa gaggaatggt tgcgcgttgc gcagctcgga ggacccggcc
     9901 gtcgagtagc gcacgtggcc gatcgccgtg gagcccggca gcttggcgag cacgtcgcgg
     9961 ttgaacgcgt cgctgaccag gcccatcgag acgtgtcggc ggaggcggcc ttcgtcggcg
    10021 gctaccagac ccgccgattc ttggccgcgg tgctggaggg cgtgcatgcc caggtacgcg
    10081 atgttggccg cttcgtcatg accatgaatg ccgaacaccc cgcacatggc gctcacttac
    10141 catgcggggc tgatcgcaag tagcaattct tgtggatcgg cgcgggttca cgtacctttt
    10201 cggggaagtg ccacggcttc tctggcgcga tgcgcagggc atcgagggct ctctggacct
    10261 tacgtccgcg gagatcaagg tcggcagagc catggactgc gccatccgca ccgacgacgc
    10321 gatggtctcg cgccatcact cgcgcatttt ctggggcggt ggcggctacg tcttggagga
    10381 tctctcgtcg gcaaacggcg tgtacttcca agagcagcgc gtccagagcc acctgttcaa
    10441 gcacggcgat gccgtccggt gcggaagtct ctggctgcgg ttcgtcgacc aggcgcagat
    10501 cggcgcgacg cccgcggctg gtcccggcac gcccgctccg atgatgcagc atccaggcat
    10561 gggcgcgatc gcgccggccg gcccgacagg tcacgtcgcc gcgatcaatc ccggcggcat
    10621 gtcggcgtcg atgcccgcgc agcagccagg cgctgcgatg gccgcactcg gcgtcgctcc
    10681 ggcgaatcag gagtctgacg ctgagattcg cgtccttcgt cgacgcgtcg agcagctgca
    10741 gaccgagctg cgcatgtatc gcagccagaa gttcaaagaa gacaacgtcc gccgcatgga
    10801 ggatctcgag aacgatctct ccatgatgga gatcgagcgc gacaatctga agacgcggct
    10861 cgacaaggcc gagcagagcc tcaagctcga agccggcagc atcaaggtga agcgcgcgct
    10921 cgagatcgcg gcgatgaccg cggagaccgc tgcgtcgctc aacgatctgc tgtcgagctt
    10981 gcgcatcgaa gtcatggcgg ccgagggcga gttcgaccag tacgcgcact cgattccacg
    11041 cgcgagcttc gagctgattc gccagtcgct caaggacgcc gcttcgcact gcgacgacgc
    11101 acgcgagctg cttcgcaagc tccgcgaagt cgccgagtaa ctcgcggtcg agccgtcagc
    11161 gaagtcgggt tcagcgcgct gtagcgtcgc aacgaaagct tcgcgtcgag ccgcgcggga
    11221 gtgacctgcg ccgcgtgctc agatcgtcac gagtaggcat tcttccgatg gtgttggcga
    11281 aaaatgaccg ttattctggt gacttccgcg atcgagattg tactcgtctg attacaacct
    11341 tggacaactg tcagcagtgc gttacatttc tgatatggcg gatgccgttc agtccgaccc
    11401 actcctacgc aggtctctct cgcgctcccg acgacgccgc gacgatctct ggctgggcgc
    11461 cagcgctctc gtcgcttcga tggcggcact gccgctcgct cagtcgagct ggcatggacc
    11521 cgaggtcgct tcgacgctcg cggttgcagc ggtcgcgctg ctcgctgggc agcggtgggc
    11581 gatctcgatc gtcgtcctcg cggagctctt gctgctgccg acgatctggc cgcgcgcgtt
    11641 tctcggcggt gtcgagctgt ggccaggtcg catcgctgcg ctgacctcgc tcgcgatgat
    11701 cgtccccggt ttgctctctg ttcgtcgcgc ggccgcggcg ctcgtgctca tcacgggctg
    11761 gcgacgcacg cagcgcacct gcaggcacct gcagtatgcg ttcggtgtgc tgttcctgat
    11821 cgcgctgctg ctgccgctga tcgcgtaacg cgactactgc ttctcgcaga agccgtagtt
    11881 gcaggcgggc gccgcgctcg tacagtccga cgcagtcgtg cacttcttac acgcggtcgc
    11941 gccgttgacc ttgttgtcga aatagcagcc gcaaccaaac ggaggcgtgt acggcgacag
    12001 tggaccgacg tcgaccgtgc gcgacacctt catcgcgcag gacggcacaa agccggcgtc
    12061 gatgatcgcc gagacgagct cttccttgag cttcggcacg ctgaactgcg tgatgaacgc
    12121 gctcgccgct tgcgagggga cgccgttcgc cgtcgacgca aagaagtgaa tcgcgcccca
    12181 gatcgggtag tgaccgtcac gcacgttcgc cttgtcgctt acctgtggag tcgagtccgg
    12241 gaggtacgcg tacttttgtc ccttctgctg aaacgcgagc aagcggaggt tcgctttcgc
    12301 tttgtccgca tagtcggccg agagaacgcc gatcacgctc tccgacaccg tcggatcgat
    12361 cgcttccatc gatgcgacga gattgccggc gctcaggcga tcgatgcccc accacatcgt
    12421 cgcgtcgact ccgatcgcgc gcgagacgag ctgcgtcgtg cccgtgcccg agctgcgcgt
    12481 gaagtagtag tgcggatcgg tccacggggt gaccttgccg cttgcgccac cggcgccgaa
    12541 cacgagatgc gctgcctcgg cgctgatcgt ggtttgtgac gacgaagacg gcacgacgag
    12601 atcgatggtc aggaccgggc cgacgtaatc ggcggtgttc gccacggccg aaaagccgca
    12661 cgcgctcgcg ttgacgtctg actcgccgac gtcgaccacg gtgccggcag gatcgagcag
    12721 acagaacgtt tgcttgccct gctggtcgta atagaacgcg tagttgttcg tgacgttttt
    12781 gatgaggtgt ttcgtcgcgt ccgggtcgag gatcgagccc gcgccacgac acgacgtctg
    12841 cggtgcgaac acagcgacgt acgagggatt gcccgcgtag agcagcggct gcacggcctt
    12901 cacgagcggc ggcaagttcg tcgaccccgt gatgtagacc atgttcggat tgacatcgac
    12961 gcagttcacg gtcggcgtcg gctgcgtgtt caccggcggc ggaacggtgc cgaggctctg
    13021 cggcgcagcg gtcgtcgtcc aggccgcatc gagcgcgcct tgatcgcaga gcccgagctt
    13081 cgcgcagttg tcgtactgga acgtctgcga cgtcgtacag cggttcgcaa actcttcctg
    13141 cgtcgtcggc tcaccaaaga agcagcccgg cggaccgaga tgcgagtcga cgcagacacc
    13201 ttcctgacag tacggatgat tcccgaacga ctcgcagtcg gaatcgacgt cgcactgcgt
    13261 gatgtctttg tcgacgtaga acgagcagcc agcgagcgct agaagaagac aggttacgcg
    13321 cgtcatcatg atgttcgacc tcaaaacgct ccggcgatcc cgattgcacc gggaccgaca
    13381 cggagctgta cagacttctc ggctttcttc gaatcgcgac gcgtcagcgt gaagtacgta
    13441 gcgattcccg ccgtcacgag tgttgcggcc atcagaccat cggcgatcgc cgcgtctcgt
    13501 accgctttgt ccttttgatc ctgcagctgc tccggcgtca ccgggtacgt gttgcgaagc
    13561 gtctcgagct tgtgcgaatc gctgcgcgca aaccacgcaa acgtgatcgc gcccgccgca
    13621 aacgcgcccg tgatcgtcca cgtcacgtag atcggcgtct tgctcggcgc ttcgtcctgc
    13681 ttcgtcaccg ccgtgacggg cgcggtgtcc ttgacgacgg gtgcggaggg cgcgaggtcc
    13741 tgccccatcg agaccgtcag ctcttggcga ccggcgacgt cgtagacgcg ctgcagagga
    13801 tcgcggcctt gaacgtggac gacgatgcgg tgctgaccgg cactgacgac caccggctcg
    13861 ggtagcggcg cgaaaccaac ggcgacatca tcgatgagga tttcagcgcc aggcgcagtc
    13921 tggatcgtca ccgagccgac gcgtcctttg aggcggtcga tctcttcgtt gacttccttc
    13981 tggcgctcgg ccggaacgtc cggg
//
LOCUS       DATLBZ010000534         5909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4398936, whole genome shotgun sequence.
ACCESSION   DATLBZ010000534 DATLBZ010000000
VERSION     DATLBZ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4398936"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2666)
                     /locus_tag="VMZ53_26795"
     CDS             complement(<1..2666)
                     /locus_tag="VMZ53_26795"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS32148.1"
                     /translation="MGEEVSARALRVKLERGEGDAAELVPLLANAAIESSTRSARLEA
                     CRLLGDIAGRALGAEWEAAERAAFSLLDAARVADTAADRRGVISAMGRGFRNIWLLPF
                     IHRRLSDKDPTIAAAALDAAGGLGFPALEEAVAAFVSEAAPVLLRRAAVSALGRMGAT
                     SAVDRLVPLIESDAADASIALAALTEIRSPAGRTAALAVLEGELEPEVQIAAVRYLAE
                     LGALEVLPMLRRLARSEDAEIRIASSLASRALKAERDRDAGERFLVALSEPDRAVRSV
                     LARRLRTLAVKDVLEQAEVLLGEDAAGVVQILGEIRDPEITRYLLALAARDSLPIPVR
                     ARAIGSIEADQQWERDELAKVAHDSKADASLRASAIQAMGAFATSTELLDKVADLAKS
                     PAPAIRGALLWALQLARRPADRSDAAKIAEIVEPMLGDADRMVRRRAIYVAGNLNLDK
                     LAAALAKCLAITGDETGETSASTSGQFSSAAISIPDILAEDIAGNPPDIRLAACVALG
                     ELGMPGVIDKVVAAIRREDDPRVLGAASNALISAAPQPAQLSALAGRVQQLLKDKDAR
                     LREAGAEMSGLLGGAVAASALTPLAADDSPSVRGAAVWALGKLADAASEKTLLGAFDD
                     DDETIHERAAAGLLRLGTPAALAQAIRFSAGHGDPAARLTIASALHVPPAHAEKLTPV
                     IDSALSKVNPDDPVFESLVRLKLATHVTQGAQASAIDVDAEITTVFPSFAQLAKLPGF
                     DQLVKSLRTAESLFQTTANQTDADLSPPITLWMKVLENYVHAWLGPRMAGLQRDPATL
                     FDYVDRVIGGSWNGYARWIEPKWKDPAEVGTAKVDIPLRSIPNAVRELQDRKRKRLDS
                     PLSVTEWARMMVLFAVDHPSSGFRNLFKLGGKS"
     gene            2698..3093
                     /locus_tag="VMZ53_26800"
     CDS             2698..3093
                     /locus_tag="VMZ53_26800"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HUS32149.1"
                     /translation="MVDRFRFPITAQPSDIDENGHVSNLVYLRWVLETATAHSIALGW
                     THARYLESGNVWVVRRHELDYLLPVTAGQQLVGETWVDPWKAASCVRRTELIREGTVV
                     ARGATTWAFMSIASGRPQRIPDEMKALFS"
     gene            3317..4861
                     /locus_tag="VMZ53_26805"
     CDS             3317..4861
                     /locus_tag="VMZ53_26805"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HUS32150.1"
                     /translation="MMGTRALFAATAAFCLASCTTTSSPNTGDDFENAARAYDVPVDL
                     LKAIAYVETRWQPVVGEAGEFERPSGAGVFALWGDNLAHGAAASGLTETAVRTDTAAN
                     IEAAAARLAELAAEQGVTGDDLMAWQPVIAGFAQIEDEEARAGYVSDVLGVLATGAET
                     VAEDGRKMTLLAHADMLVPQVTTIAGSSVDFGSAISRPSPNFNSRNGSAVSLVVIHDC
                     EGSYSGCWGYLRTTAAQASAHYVVNESGSEVTQLVRESNRAWHVAANYQCANAGNAQC
                     GKNGTSTNTFSVGIEHAGFASQASWSNGIIEKSAALTCDITKRHGVIRDRNHIVSHGQ
                     LQPYNRNDPGPNWPWSHYIDRVREICGDTGGGGGGGGNPTQAIIVDSNNANNNQAVAK
                     IELTGTWTASTSNAGYYGSGYWTANTAQSSAPATFYFYLPAAGTKTIDGWWTTGTNRA
                     TATPFIAFNASGTEVGRKLVNQQTGGSQWVALGTWNFSAGWNKVVVSRWTNSGSVVIA
                     DAIRVR"
     gene            4858..>5909
                     /locus_tag="VMZ53_26810"
     CDS             4858..>5909
                     /locus_tag="VMZ53_26810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32151.1"
                     /translation="MIAPRPHIAVAARANIAAASHALSARFARLATAISLLALVACSA
                     GDEPAPSKSRVELPGTLWMMRGTVLTRYAGGLATPIADSIYPSTSALPDGRIVAIASR
                     GDGETGEQLALVSPGGRITKLGPVAAQVRDPAVVRDATGVASIVVAMNVGGQSDLYRV
                     ALDGTTTRLTNDAAGNYHPAAAASRDAAGNDIVYVSSRDGDAEIYRTALNESAVDQSA
                     QRLTAFHKDDFDPVPSPDGQTIVFSSDREGPVRLFVMASDGTKLQRVTARTDAIDESD
                     AVFSRDGSMLAYLAGGRVFVRGSGTEHSAAIEREIGAGISATFSPDGRWLAVVTATGM
                     LSVFPLNGGEPVPVAQG"
BASE COUNT          977 a         1943 c         2051 g          938 t
ORIGIN      
        1 gacttgccgc cgagcttgaa gaggttgcgg aagcccgacg acggatggtc gacggcaaag
       61 aggaccatca tgcgcgccca ctcggtcacc gacagcggtg agtcgaggcg cttgcgcttg
      121 cgatcttgca gctcgcgcac ggcgttgggg atcgaacgca gcgggatatc gaccttcgcg
      181 gtgccgactt ccgcggggtc tttccacttc ggctcgatcc agcgcgcgta gccgttccag
      241 ctgccgccga tcacgcggtc gacgtagtcg aacagtgttg cggggtcgcg ctgaaggcca
      301 gccatgcgcg ggccgagcca cgcgtgcacg tagttctcga gcaccttcat ccagagtgtg
      361 atcggcggcg agaggtcagc gtctgtctgg ttcgcggtcg tttggaacag cgactcggcg
      421 gtgcgcaggg acttcacgag ttggtcgaaa ccgggcagct ttgctagctg cgcgaagctc
      481 gggaacaccg tcgtgatctc ggcatcgacg tcgatcgcag aagcctgcgc gccttgcgtg
      541 acgtgcgttg cgagcttgag gcgcacgagc gactcgaaca ctggatcgtc gggattgacc
      601 ttcgacagcg cgctgtcgat gacgggcgtg agcttctccg cgtgtgccgg cggaacgtgc
      661 agcgcagacg cgatcgtcag gcgcgcggct ggatcgccgt ggccggcgga gaagcggatc
      721 gcttgggcga gcgcggcggg cgtgccgaga cggagcaggc cagcagcggc gcgctcgtgg
      781 atcgtctcgt cgtcgtcgtc gaatgcgccg agcagcgtct tctcgctggc ggcgtcggcg
      841 agcttgccga gcgcccacac agcagcgccg cgcaccgacg gagaatcgtc ggccgcgaga
      901 ggcgtcagcg cgctcgcagc gacagcgccg ccgagcaggc cggacatctc ggcgcccgcc
      961 tcgcgcaggc gcgcgtcctt gtctttgagc agctgctgta cgcgacctgc gagcgcggac
     1021 agctgcgcag gttgcggcgc ggcggagatc agcgcgttcg atgcggcgcc aagcacgcgc
     1081 ggatcgtctt cgcggcggat cgcagcgacg accttatcga tcacaccggg catgccgagc
     1141 tcgccgagcg cgacgcacgc agcgagacgg atgtcaggtg gattgcccgc gatgtcctcg
     1201 gcgaggatgt ccggtatcga gatcgcggcg ctcgagaact ggcccgatgt gctcgcgctg
     1261 gtctcgccgg tctcgtcgcc ggtgatcgcg aggcactttg cgagtgcggc ggcgagcttg
     1321 tcgagattga ggttacccgc gacgtagatc gcgcgacggc gcaccatccg gtctgcatcg
     1381 ccgagcatcg gctcgacgat ctctgcgatc ttcgctgcgt cgctgcggtc ggctggacgc
     1441 cgcgcgagct gcagcgccca caggagcgcg ccgcggatgg cgggagccgg cgacttcgcg
     1501 agatcggcga ccttgtcgag cagctctgtc gatgtcgcga acgcgcccat cgcctggatc
     1561 gccgacgcgc gcagcgatgc atcggctttg gagtcgtgcg cgaccttcgc gagctcgtcg
     1621 cgttcccact gctggtcggc ctcgatcgat ccgatcgcgc gggcgcggac cgggatgggc
     1681 agcgagtcgc gcgcggcgag ggcgaggagg tagcgcgtga tctccggatc gcggatctcg
     1741 ccgagaatct gcacgacgcc cgcagcatct tcgccgagca gcacctcggc ttgctcgagg
     1801 acgtctttga cggcgagcgt gcgcaaccgg cgcgcgagga ccgagcggac ggcgcgatcg
     1861 ggctcgctga gcgcgacgag gaatcgttcg ccggcgtcgc gatcgcgttc ggctttgagt
     1921 gcgcgagagg ccaggctcga tgcgatccgg atctcggcgt cctcggagcg cgcgagtcgc
     1981 cgcagcatcg gcagcacttc gagcgcgccg agctcggcga ggtagcgaac ggcggcgatc
     2041 tgaacctcgg gctcgagctc gccttcgagc acagcgagcg cagcggtgcg acctgccggc
     2101 gagcggatct cggtcagcgc ggcgagcgcg atcgatgcat ccgcagcatc actctcgatg
     2161 agcggcacga gccggtcgac ggcggacgtc gcgcccatgc ggccgagcgc ggagactgcc
     2221 gcgcgccgga gcaagaccgg tgctgcctcg ctgacaaacg cagcgaccgc ctcttcgagt
     2281 gcgggaaagc cgaggccacc tgcggcgtcg agcgcggcgg cggcgatcgt cggatccttg
     2341 tcggagagcc ggcgatggat gaacggaagg agccagatgt tacggaagcc gcggcccatt
     2401 gccgagatga cgccgcgccg atcggccgcg gtatccgcga cgcgcgcagc gtctagcagc
     2461 gagaacgcag cacgctccgc cgcttcccac tcggcaccga gcgcgcggcc ggcgatatca
     2521 ccgaggagac ggcatgcttc gagccgtgcc gatcgcgtcg acgattcgat cgccgcattc
     2581 gcgagcagcg gcacgagctc ggctgcatcg ccttcgccac gctcgagctt cacgcggagc
     2641 gcacgtgcgc tgacttcttc acccataccg ggacgttaac aggtaggctg cccggccgtg
     2701 gttgaccgct tccgctttcc gatcaccgct cagccctccg acatcgacga gaacggtcac
     2761 gtctcgaacc tcgtctacct gcgctgggtg ctcgagacgg ccaccgcgca ctcgatcgcg
     2821 ctcggctgga cgcatgctcg ctacctcgag agcggcaacg tctgggtcgt gcgccgtcac
     2881 gagctcgact accttctccc cgtgaccgcc ggccagcagc tcgtcggcga gacgtgggtg
     2941 gatccatgga aggccgcgtc atgcgttcgt cgcaccgagc tcattcgcga aggcactgtg
     3001 gtcgctcgcg gcgcaacgac gtgggcgttc atgtcgatcg cgagtggccg tcctcagcga
     3061 attcctgacg agatgaaggc gctgttctcc tgactgtcag cacgcgcatt tgctagtgcc
     3121 ggtcagaagt tggtggtcgc tgtaggtctt gaacgatcgt caggcagcag gtttttttgg
     3181 acactgcaca cggtcgtgag cagccgaacg gtggttcgga atccgacgcg cgcaactcgt
     3241 gtcggagcct gcgcattcat ccgcctacag agtgcgatcg agtgggtggc atctaacgtg
     3301 aagtgcgtcg cacgcgatga tgggaacccg cgcactgttc gcggcaacgg ccgcgttttg
     3361 tctcgcgtcg tgcacgacga cgtcttcgcc taacacaggt gacgacttcg aaaacgctgc
     3421 tcgcgcctac gacgtaccag tcgatctgct caaagcgatc gcgtacgtcg aaacgcggtg
     3481 gcagcccgtc gtcggtgaag cgggcgaatt cgaacggccg agtggcgctg gcgtgttcgc
     3541 actctggggc gacaacctcg cgcatggcgc cgctgcgtcc ggcctgaccg aaaccgccgt
     3601 ccgcacggac accgccgcca acatcgaagc cgctgcggcg cggctcgccg agctcgcggc
     3661 cgagcaaggc gtcacgggtg acgacctcat ggcgtggcaa ccggtcatcg ctggcttcgc
     3721 gcagatcgag gacgaggaag cgcgcgctgg ctacgtgagc gacgtgctcg gcgtgctcgc
     3781 gacgggcgca gagacggtcg ccgaagacgg ccgcaagatg acgttgctcg cgcacgccga
     3841 catgctcgtc ccacaggtca cgacgatcgc cggctcctcg gtcgacttcg ggagcgctat
     3901 ctcgcggccc tcgccgaact tcaactcgcg caacggcagc gcggtctcgc tcgtcgtgat
     3961 ccacgactgc gaaggcagct actccggctg ctgggggtac ctccgcacga ccgccgcgca
     4021 agcaagcgcg cactacgtcg tcaacgagag tggcagcgaa gtcacgcagc tcgtgcgcga
     4081 gagcaaccgc gcgtggcacg tcgccgcgaa ctaccaatgc gcaaacgcgg gcaacgcgca
     4141 gtgcggcaag aacggcacgt cgacgaacac gttctccgtc ggcatcgagc acgcgggctt
     4201 cgcgtcgcaa gcgagctgga gcaacggcat catcgagaag tccgccgcgc tcacgtgtga
     4261 catcacgaag cgccacggcg tgatccgcga tcgcaaccac atcgtgtcgc acggccagct
     4321 ccagccgtac aaccgcaacg atccaggtcc gaactggccg tggagccact acatcgaccg
     4381 cgttcgcgag atctgcggtg acacgggcgg tggcggtggc ggcggcggca acccgacgca
     4441 agcgatcatc gtcgacagca acaacgcgaa caacaaccag gcggtcgcga agatcgagct
     4501 gacgggtacg tggaccgcgt cgacgtcgaa cgccggttac tacggcagcg gttactggac
     4561 cgcaaacacg gcgcagtcat cggcgcccgc gacgttctac ttctacctgc cggccgccgg
     4621 cacgaagacg atcgacggtt ggtggacgac gggtacgaac cgcgcgacgg cgacgccgtt
     4681 catcgcgttc aacgcgagcg gcactgaagt cggccgcaag ctggtcaacc aacagacggg
     4741 tggcagccag tgggtcgcgc tcggcacctg gaacttcagc gccggctgga acaaggttgt
     4801 cgtgtcgcgc tggaccaaca gcggctccgt cgtgatcgcc gacgcaatcc gcgttcgatg
     4861 atcgcacctc gcccgcacat cgcggtcgcc gcgcgcgcga atatcgcggc ggcatcgcac
     4921 gcgctcagcg cgaggtttgc gcgtctcgca accgcgatct ccttgctcgc gcttgtcgcc
     4981 tgcagcgctg gcgatgagcc tgctccgtcg aagtcgcgcg tcgaattgcc gggcacgctc
     5041 tggatgatgc gcggaacggt gctgacgcgt tacgcgggtg ggctcgccac gccgatcgcc
     5101 gacagcatct atccgtcgac gagtgcgctt cctgacggcc gcatcgtcgc gatcgcgtcg
     5161 cgtggtgatg gcgagacggg cgagcagctc gcactcgtgt cgccgggcgg gcgcatcacg
     5221 aagctcggcc ccgttgctgc gcaggttcgc gatccggcgg tggtgcgcga tgcgacaggc
     5281 gtcgcgagca tcgtggtcgc gatgaacgta ggcgggcaga gcgacctcta tcgcgtcgcg
     5341 ctcgacggca ccacaacgcg gctcacgaac gacgccgccg gcaactacca ccccgcggct
     5401 gcagcgtcgc gcgacgcggc aggcaacgac atcgtctacg tgtcgagccg cgatggcgac
     5461 gcggagatct acagaactgc attgaatgag agcgccgtcg atcaatcagc gcagcgactc
     5521 accgcctttc acaaagacga cttcgatccc gtgccgtctc ctgacggcca gacgatcgtc
     5581 ttctcgagcg atcgtgaggg cccggtgcgc ctcttcgtga tggcgagcga cggcacgaaa
     5641 ttgcagcgtg tcacggcgcg caccgacgct atcgacgaga gcgacgccgt gttttcgcgc
     5701 gacggctcga tgctcgcgta tctcgcgggt ggtcgcgtgt tcgtgcgtgg cagcgggacc
     5761 gagcacagcg ccgcgatcga gcgcgagatc ggcgctggga tctcggcgac gttttcgccg
     5821 gatggccggt ggctcgccgt ggtgactgca accgggatgt tgtcggtttt ccccctcaat
     5881 ggaggggagc cggtcccggt cgcgcaggg
//
LOCUS       DATLBZ010000535         5482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1442563, whole genome shotgun sequence.
ACCESSION   DATLBZ010000535 DATLBZ010000000
VERSION     DATLBZ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442563"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VMZ53_26815"
     CDS             complement(<1..226)
                     /locus_tag="VMZ53_26815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32152.1"
                     /translation="MSRTGANTVVEPVRLSATGAEPVRLSATGSKLVRRSATGAELVR
                     LSATGAELVRLSATGAELVRLSAAGSKRAAI"
     gene            237..2039
                     /locus_tag="VMZ53_26820"
     CDS             237..2039
                     /locus_tag="VMZ53_26820"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HUS32153.1"
                     /translation="MVEQLVKKGRIARVRIAGNERLIAAEDAARYRDGLGINIPPGLP
                     AAFLDKAESPVESLIARWARTHSPFTAAEPAARWGIPAPQIEPVLALLEARGQLVRGE
                     LRPGGKGLDACDPEVLRQLRRRTLAKLRAQVAPVGSSAYAGFLPRWHGLDQPRRGPLA
                     LRDAIGRLEGVALPFSELEQRILPARILDYHPRMLDELGAAGELTWVGAGSLGTKDGR
                     VMLLRRDRARELAPEPQEIVNRSAIHDAIIAHLKSSGASFLVAIESAVRTYSGSANGS
                     AGAGGVGAGGVGGSAGGDRSAAVISDRSAAVIGDRSAIQAAMWDLVWAGLITNDTFAP
                     LRSLSAPTSRRMNAGASFGGRWSLVESLGPAPTATARAHAQATALLERWGVASRVGAK
                     ADELPGGFSTIGDVMRAMEDAGTVRRGYFVEQLEGAQFAWPGAIDRLREAPRGQAQRV
                     DVLAAVDPANAWGSALPWPALRDADARPARRVGGTVVLVDGALALWVEPKGRRIATAA
                     ELPAEQIELALAVALPRIAMKARRRELLIETIDGESAQQSAWARSLLAAGARIDYRGL
                     VVRGSVVTAPPPDAETEPADAADDEAEEDEAADA"
     gene            2036..2848
                     /locus_tag="VMZ53_26825"
     CDS             2036..2848
                     /locus_tag="VMZ53_26825"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HUS32154.1"
                     /translation="MSAGGACGVMPEGDSIRRVASRLQPLIGQTLARVTTQGIVRNLV
                     GRTITAVAPHGKHLMIDLDDGTQLRMHLGMNGRVRYYMREDGEAAVARISPGRASLVL
                     VTAEYVAMWIQAPTVEIAPRRAPMRGIAVAALGPDVLADDFDANVAATRALEAAHRMI
                     GEVLLDQRVAAGIGNIWRCESLFVTRIDPRALVGSLSHDQIAATYRAARELMLTRLTA
                     AGPARDRFAVYSRTNKPCPRCEAPIRAYQLGEPPRWTWSCPVCQPHGASGGA"
     gene            2993..3460
                     /locus_tag="VMZ53_26830"
     CDS             2993..3460
                     /locus_tag="VMZ53_26830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32155.1"
                     /translation="MATALAYPAQAPLSSEVSTTLTVGAGAAFEVFADAVEIPRWLPL
                     VQTARVLSTRPDGRPSRVAFTRKLERGSLGYTLEYSYDPSTYTISWSTPGTSNVVLSG
                     EARFVPLSQRACLMLYRLVLDLPIVDDLITTELDGHPASLVVAEFREHLRRLC"
     gene            3683..4423
                     /locus_tag="VMZ53_26835"
     CDS             3683..4423
                     /locus_tag="VMZ53_26835"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HUS32156.1"
                     /translation="MHAGEVERLRELEDKLGRCIATLEIHMQPIVHATTRARFGYEAL
                     MRSTDKSLPHPGAVLDAAERLEKVPQLGRTLRSHTAKIIANAPGGRGLFFVNLHLLDL
                     LDKQLTSAFAPLTKVASRVVLEITERTSLQGHDEIRYRVAELRELGFKIAIDDLGGGH
                     ARMGTFSPFDTDFVKLDMSLVRDLEKHPTKQRLVKSIIELCAEGGTKVVGEGVETEAE
                     AQVLVELGCDLLQGYLIARPQLPFVDPL"
     gene            4447..>5482
                     /locus_tag="VMZ53_26840"
     CDS             4447..>5482
                     /locus_tag="VMZ53_26840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32157.1"
                     /translation="MRRLLLLGLMACSPTRDDVVGPFTGQPRRFYVDRIDVPRDNAQA
                     LAVAADLDGDDAVENQFGNVTGVLASTNDLSTNSADMIAAGSLASYVDIQADDLMNDA
                     AVGITYVGDESELGQTPFGGKFVDGAFISNRTRDTEYPGKTTVHLPVYTNANPLVLLV
                     EGAELELTPDGDGYTGILRGGLREETAREAAYFGLIDMFQNEPERHLVFGRGIDANHD
                     GAVSREEVDASVIALLVTADVDLFDGSRLAPHAGSQDNDCISVAFQFHLSPTPPTGPA
                     QNTCRDRMGDGDESGNDCGGSCQKCWDHSPCAIDADCQSGACIPGGPLEGTGKWCAEA
                     TCTDGVRDAFE"
BASE COUNT          860 a         1800 c         1903 g          919 t
ORIGIN      
        1 aaatcgccgc gcgcttcgag ccagcggccg acagccggac aagctccgcg ccggtggccg
       61 acagccggac aagctccgcg ccggtggccg acagccggac aagctcggca ccggtggccg
      121 accgccggac aagcttcgag ccggtggccg acagccggac aggctccgca ccggtggccg
      181 acagccggac aggctcgaca acggtgttag cgccggtacg cgacatcgtt gcgccgatgg
      241 tcgagcagct cgtcaagaag gggcgcattg cgcgggtgcg cattgccggc aacgagcgcc
      301 tcatcgcggc cgaggatgcg gcgcggtatc gcgatgggct cggcatcaac attccgcccg
      361 ggttgcccgc ggcgtttctc gacaaggccg agtcgcctgt cgagtccttg atcgcgcgct
      421 gggcgcgcac gcattcgccg tttaccgctg ccgagccggc tgcgcgctgg ggcattcctg
      481 ctccacagat cgagcccgtg ctggcgttgc tcgaggcccg cggccagctg gtccgcggag
      541 agctgcggcc gggaggcaaa gggctcgacg cgtgcgatcc ggaagtgctg cggcagctgc
      601 gccggcggac gttggcgaag cttcgtgcgc aggttgcgcc cgttggctcg tcggcgtacg
      661 cgggattttt gccgcggtgg catggcttgg atcagccgcg tcgcgggcca ctcgcattgc
      721 gcgatgcgat tggccggctc gagggcgttg cgctgccgtt ctcggagctc gagcagcgca
      781 ttcttcctgc gcgcattctc gattaccacc cgcgcatgct cgacgagctc ggtgcggccg
      841 gcgagctcac gtgggtcggc gctggatcgc tcggtaccaa agatggtcgc gtgatgctgc
      901 tgcgccgcga tcgagcgcgc gagctcgcgc cggagccgca agagatcgtg aaccgctcgg
      961 caatccacga cgcgatcatc gcgcacttga agtcgagcgg cgcgtcgttc ctcgtcgcga
     1021 tcgagagcgc agttcgcacg tactcgggat ccgcgaatgg gagcgcgggc gcgggcggcg
     1081 tgggcgcggg cggcgtgggc ggcagcgcgg gcggcgatcg cagcgcggct gtcatcagcg
     1141 atcgcagcgc ggctgtcatc ggcgatcgca gcgcgattca ggctgcgatg tgggacctcg
     1201 tctgggccgg cctcattacg aacgacacgt ttgcgccgct gcggtcgctg agcgcgccga
     1261 catcgcgtcg catgaatgcg ggcgcgagct ttggcggccg gtggagcctc gtcgagtcgc
     1321 tcggtcccgc gccgacggcg acggcgagag cgcacgcgca ggcgaccgcg ttgctcgagc
     1381 gctggggcgt tgcgagccgc gtgggcgcga aggccgacga gctgccgggc gggttctcga
     1441 cgatcggcga tgtcatgcgc gcgatggaag acgcgggcac cgtgcggcgt ggttactttg
     1501 tcgagcaact cgaaggcgcg cagttcgcgt ggcctggcgc gatcgatcgg ctgcgcgagg
     1561 cgccgcgcgg gcaagcgcag cgcgtggatg tgctggctgc ggtcgatccg gcgaatgcgt
     1621 ggggctctgc cctgccctgg cccgctcttc gcgatgcgga tgcgcggcct gcgcggcgcg
     1681 tcggcggcac ggtggtcctc gtcgatggtg cgctcgcgct gtgggtcgag ccgaaggggc
     1741 gtcgcatcgc gactgccgcg gagctgcccg cggagcagat cgagctggcg ctcgccgttg
     1801 cgcttccgcg catcgcgatg aaggcgcgcc ggcgcgagct gctcatcgag acgatcgacg
     1861 gcgagtcggc gcagcaatcc gcgtgggcgc gcagcctgct cgcggccgga gcgcgcatcg
     1921 actatcgcgg gctcgtcgtt cgcggcagcg tcgtcacggc gccaccgcct gacgcggaga
     1981 ccgagccagc agacgcggcg gacgacgaag cagaagagga tgaggccgcc gatgcgtgag
     2041 cgcgggcggg gcgtgcggtg tcatgcctga aggcgacagc attcggcgcg tcgcgagccg
     2101 cttgcagccg ctcatcggtc agacactcgc gcgtgtcacg acgcagggca tcgtccgcaa
     2161 cctcgtaggg cgcaccatca ccgccgtcgc gccgcacggc aagcacctca tgatcgatct
     2221 cgatgacggc acgcagctgc gcatgcacct cggcatgaac ggccgcgttc gttactacat
     2281 gcgcgaggac ggcgaagcgg cggtcgcacg catctcccca ggccgcgcga gcctcgtgct
     2341 cgtcaccgcc gagtacgtcg cgatgtggat ccaagcgccg accgtggaga ttgcaccgcg
     2401 gcgagcaccg atgcgcggca tcgcagtcgc ggcgcttggc cccgacgtcc tcgcagacga
     2461 cttcgacgca aatgtagcgg cgacgcgcgc actcgaggca gcacaccgaa tgatcggtga
     2521 ggtgcttctc gatcaacgcg tcgcagcggg catcggaaac atttggcgct gtgagtccct
     2581 ctttgtgacg cgaattgatc cgcgcgctct cgtcggctcg ctctcccatg atcagatcgc
     2641 cgcgacgtat cgcgctgcgc gcgagctgat gctcacgaga ttgacggctg ctggaccagc
     2701 cagagatcgc ttcgccgttt acagccgcac gaacaagccg tgcccacgat gtgaagcacc
     2761 gattcgcgcg tatcagcttg gcgaaccgcc gcgttggacg tggtcgtgtc cggtctgcca
     2821 gccacatggg gcttcgggtg gggcctagtg ttagcggaca ccgatcgcgc cgcggctagc
     2881 cgagtggtcg gtggagtggc tcccgaatgg gtttcaacta ggcggaacct ggtcgctgga
     2941 agctggtatc ggggttgctc attgggtcgg caccccgaag gagtaacgca ccatggctac
     3001 cgcgctcgca tatcccgccc aggcccctct ctctagcgag gtctcgacga cgcttacggt
     3061 aggcgcggga gcggccttcg aggtctttgc ggacgcggtc gaaatcccgc gctggctgcc
     3121 gctggtccag acagcccgcg tgctgtcgac gcgccccgac ggccgcccga gccgcgttgc
     3181 gtttacccgc aagctcgagc gcggatcgct cggctatacg ctcgagtaca gctacgaccc
     3241 gtccacctac accatctcgt ggtcgacacc cggaacgtcg aacgtggtgc tttcgggcga
     3301 ggcgcggttc gtgccgctgt cacagcgcgc gtgcctcatg ctttaccggc tcgtcctgga
     3361 tcttccgatc gtcgacgacc tcatcacgac cgagctcgat ggccatccgg catcgcttgt
     3421 cgttgcagag ttccgcgagc acctgcgtcg cctctgctag gcctacatga tccggtaacg
     3481 ttgggttagg gcggctcact cagggattgg cgagtcgccc tcgctctggg tacagtgaat
     3541 gtctgtgccc gcggagaagc gtccagccac gctcgctgct ttactgccgc cgcctcgcgc
     3601 gcggacctca ccaccaactc cgaccgagga ggccgtcgcg ccccttgctc gaggtacgga
     3661 tccccagcta agccgtctgg acgtgcacgc aggtgaagtc gaacgcctac gcgagctcga
     3721 agacaagctc ggtcgctgca tcgcgacgct cgagattcac atgcagccga tcgtccacgc
     3781 gacgacgcgc gcacgcttcg gctacgaagc gctcatgcgg tcgaccgaca agtcgctgcc
     3841 gcatcccggc gcagtgctcg atgctgccga gcgtctcgag aaagttccgc agctcggtcg
     3901 cacgctgcgc agtcacaccg cgaagatcat cgcgaacgca ccgggcggtc gcggcctgtt
     3961 cttcgtcaac ctccacttgc tcgaccttct cgacaagcaa ctcacgagcg cattcgcgcc
     4021 gctgacgaag gtcgcatcgc gcgtggtgct cgagatcacc gagcggacgt cgctgcaggg
     4081 ccacgacgag attcgctacc gcgtcgcgga gcttcgcgag ctcggcttca agatcgcgat
     4141 cgatgacctc ggcggcggcc acgctcggat ggggacgttc tcgccgttcg acaccgactt
     4201 cgtgaagctc gatatgtcgc tcgtgcgcga cctcgagaag cacccgacga agcagcggct
     4261 cgtcaaatcg atcatcgagc tctgcgccga aggcggcacg aaggtcgtcg gagaaggcgt
     4321 cgagaccgag gccgaggcgc aggtcctcgt cgagctcggc tgcgacttgc tccagggcta
     4381 cctgatcgct cgcccgcagc tgccgtttgt cgacccgctc tgacgggtgg gtgatagcgt
     4441 cggggcgtga ggcggttgct gctgctcggg ctgatggcgt gctcgccgac gcgcgatgac
     4501 gtcgttggtc cgtttaccgg acagccgcgc cgcttctacg tcgatcgtat cgatgtcccg
     4561 cgtgacaacg cgcaagcgct cgcagtggct gccgacctcg atggcgatga cgccgtcgag
     4621 aatcaattcg gcaacgtcac gggcgtgctc gcgagcacga acgatctctc gacgaacagc
     4681 gccgacatga tcgcggccgg gtcgctggcg tcgtacgtcg atatccaggc cgacgatctg
     4741 atgaacgacg cggctgtcgg catcacgtac gtcggcgatg agtcggagct cggccaaact
     4801 ccgttcggtg gcaaattcgt cgacggtgcg ttcatatcga accgcacgcg cgatacggag
     4861 tatccgggca agaccacggt ccacttgccg gtctacacga acgcgaatcc gctcgtcttg
     4921 ctcgtcgaag gcgccgagct agagctgact cctgatggtg acggctatac cgggattctt
     4981 cgcggcggcc tgcgcgagga gactgcacgc gaagcggcgt actttggcct gatcgacatg
     5041 tttcagaacg agccggagcg gcatctcgtg ttcggccgcg gcatcgacgc gaatcacgat
     5101 ggcgcggtct cgcgcgagga agtcgatgcg tccgtgatcg cgttgctcgt cacagcggat
     5161 gtcgatctgt tcgacggctc gcgcttggca ccgcatgccg gatcgcagga caacgactgc
     5221 atctcggtgg cattccaatt tcatctctcg ccaacgccac cgaccggacc ggcgcaaaac
     5281 acctgtcgcg atcgcatggg cgatggcgat gaatcgggga acgactgcgg cggctcgtgc
     5341 cagaagtgtt gggatcactc gccttgcgcg atcgacgccg attgtcagag cggcgcgtgc
     5401 attcccggcg ggccgctcga agggaccggc aagtggtgcg cggaggcgac gtgcaccgac
     5461 ggcgtccgcg atgcgttcga ga
//
LOCUS       DATLBZ010000536         9564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1442979, whole genome shotgun sequence.
ACCESSION   DATLBZ010000536 DATLBZ010000000
VERSION     DATLBZ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442979"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VMZ53_26845"
     CDS             complement(<1..1212)
                     /locus_tag="VMZ53_26845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS32158.1"
                     /translation="MGALAVRGLRVALPDGRVLIDGIDLSIGEGEVIVLLGGSGAGKS
                     TFARVLFERDELAAAGFDVVAAQLDLDRGQLGLVPQRGALFDHLDVRGNLELALRHAR
                     SDEKAEDWLERVGLSRELAEPGTPVTALSGGQAQRVAVARVLASKRKLLFLDEPSVGL
                     DPHRVRVLARLIREQVKALGISAIVVTHDVALAAGVADKLFMLSLADRKLEQLFADRW
                     PGALEDPGHSAEERGRWLVELEDVLVKHLEEHGDRAPPAGAPPRRGALRSMGRLVDPL
                     LAPFRVAALSLWKAPVQLVRHPRDFAIVSWRVVKQTLLRPLPFYAIVSTLIGYTVLFV
                     ISKVGGAGVKPEALLRQIGGSHVVALAPALSALLFVAASGSATNAWLGSMGLTKQTLA
                     LDALGVDRRSYL"
     gene            1387..3090
                     /locus_tag="VMZ53_26850"
     CDS             1387..3090
                     /locus_tag="VMZ53_26850"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS32159.1"
                     /translation="MSAQQDFFGEKAVEVATLLGDSVVDVKHCMDPHGGKVTPRTWAM
                     LATGAACLLLSAGAFIVSVKTAAYNHGAQDYWTHVAHKPQGAFRGVVLSGAYDWLAFG
                     GFLLGLLTVAVGLYRARKEKQDPTYKIGTASDVRVAVEGAPAPSFAMVAPKGDDFVFN
                     YGVGMTGEMLIDGKTIPLAELAASGHARPSTSAVGAFELPIPMNGRIRANIGLTTFQV
                     SAVAKPQAQSSPMFAMSSRTLSYFAGSLGAHLAAVLLLAQIPMDDGSASFDLAALEPT
                     DIRSNNGINEDVPPTPPEDVANNTGDGKEASGKSMQLEEGQAGTTKSNNQQSHIRIAN
                     NNREDTFAQRQVEIENARQAGILGSAALQDGSLFADLASQSEIASGPDSSNVWGAIYG
                     ADGEGYGTFGYGRHGFGPGGGCYGTDCGLIGTPNGYGKIGLGKFPGSGWDGGGGGPPM
                     RRRHTPTVPGPVVGEAKIVGGLDKAIIRRYIKRNIDKISYCYEKQLLANPSIEGTVKI
                     QFFISPTGAVTSSTGEGFNGAVASCVAGVVSAIEFPRPDGGGGVTVNYPFTFHASGSG
                     S"
     gene            3170..4459
                     /locus_tag="VMZ53_26855"
     CDS             3170..4459
                     /locus_tag="VMZ53_26855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32160.1"
                     /translation="MSLEEFGSYVVLEQLGVGGMASVHVAESRSMGGFRKRVALKRLL
                     PHAAENPDLVRSFLDEARLNTYLKHPNVAQIYDFGQVGDVYFMAMELVAGPTLNQLAR
                     QCDSTVGIIPFPITMNILIQVLDALDYAHNLRDEHGKRLGIIHRDVSPPNIVVSNQGL
                     VKLIDFGIAKAASSSVQTQVGTVKGKFSYMAPEYLREKLDHRVDIWAVGVIAHELLTN
                     RRLFDANDDFKVIDMVKELRIDPPSRRNVDVPPDLDAVVMTALQRDPDQRWQSAAAMR
                     TALANAAAELQTVVTNAQLIEWVEWTFSQKPPGEGSDLSQLIQILETPSRPSARLSNP
                     DEPTVKGRKQSAQPVKSERYFARALNAFSTPKAGIAAVKRAFSTPQVGAAMVKRNSSG
                     LRFVTTLLFLLLLGAIGVGIAAWFASWPFDLQQLLLG"
     gene            complement(4461..5135)
                     /locus_tag="VMZ53_26860"
     CDS             complement(4461..5135)
                     /locus_tag="VMZ53_26860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS32161.1"
                     /translation="MRTSLVLALVACAGPARRETPAQQPRAVPPDASVASVSTAPSLP
                     IDAAVDAPAVASPAIADAGIARIDPLPEDPEQWPMLAAMGNARAADALAKLPPNQRPC
                     EGTGSMSCDLSSNVSPGAGGKKTMLSGQVSKGFVSGVPGHEGHAMNVAACKVVAIDAK
                     THKTHTAQVNAKGQYSLGVPAGSYQVVFDDCFECVVKYHPKLEVLTLKAGDWGTASAQ
                     CRVLGK"
     gene            complement(5178..6035)
                     /locus_tag="VMZ53_26865"
     CDS             complement(5178..6035)
                     /locus_tag="VMZ53_26865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32162.1"
                     /translation="MKGTIAIAVAAAVLLFHLGRAPLAHAEPTEKELSARAAELEKKL
                     KGQGFTVLVEAPFVVVGDSGDKYVKKITTGFIRSKVALLEKDFFAKRPDRVLEVWLFH
                     NEKSFRAGAKKYFGDTPDTPYGYYSPDANALIMNASGLGTLSHELVHPYMEANFPDVP
                     SWFNEGLASLYEQPGEVKGHIVGFPNWRLPNLKKEIKAGTLPKLSKLLGTTRDQFYEA
                     DYDSYAYARYLVYYLQEQGKLQEFYKKFLEDKKDLTGATALAAVLGEDLEKFEPKWRK
                     WVMAIKYKR"
     gene            6119..6664
                     /locus_tag="VMZ53_26870"
     CDS             6119..6664
                     /locus_tag="VMZ53_26870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32163.1"
                     /translation="MVSVPWIAGLLVVATLVNWLRKRSWADRVKGPGLYAAVGAGAGL
                     VALVVALLIGTPVVEGLTDQAVQWSTYPVVRGSVTTFVMVAIVIAAGSLACELVLRGF
                     LVELGREFTRSNAVAVLMGGFAEALLADGDAGMKLGAGVFGVGLGAIYIASGRSVVAP
                     LCARLVFLLGALVLEALRVVG"
     gene            6675..7196
                     /locus_tag="VMZ53_26875"
     CDS             6675..7196
                     /locus_tag="VMZ53_26875"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS32164.1"
                     /translation="MSVTVREATPADAAVLIAHLQELVGEPGINIPLAPDEVTMTVEQ
                     EKAILADFAASPRALMLVATGTEGGVSQIVGELSLKAISSRKAVQHVATLGMSVKQGW
                     RRKGVGRELITAALEWAPSAGIKRVELYVYARNVPAIALYEKCGFAIEGRRTAFIREG
                     ETFLDDLVMAKIL"
     gene            complement(7263..7565)
                     /locus_tag="VMZ53_26880"
     CDS             complement(7263..7565)
                     /locus_tag="VMZ53_26880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32165.1"
                     /translation="MTFRKLVALAGIGGLMYAHRKRGGEFTVASIKQSWTDLISGAKN
                     KAADLRTQAESRLHDAASKVSSATDVSSQQSFSGGAADVTGYGSSGYGYGGGSTNR"
     gene            7824..8603
                     /locus_tag="VMZ53_26885"
     CDS             7824..8603
                     /locus_tag="VMZ53_26885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32166.1"
                     /translation="MEVFFLMMGLWLVVAVALGIRRPRGLGGAALRARLDAVYGEPHE
                     LVQVSPGGFPEADIAFYDRVKSALEQKGYSTLGDVEDLTLSRIYPHNRTFLRLFVDAG
                     RMIRASAYHLHPRGVVISLLQLVQLWPRHLRVVELVSEVGGKFLVTSNTHGVDRLEPP
                     PEAKIERLPLETSFLEVVARHEARITAYLKEHPERAPTSFERLDDVLASIARAHLAMA
                     RHRQKVGGLSRDELERLKGRPLSATEEAFLREVQAGKTEPS"
     gene            complement(8629..9039)
                     /locus_tag="VMZ53_26890"
     CDS             complement(8629..9039)
                     /locus_tag="VMZ53_26890"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HUS32167.1"
                     /translation="MPRMTIAEIEKLMDTGFPEWRKFSAITALGDNDITLTMPFRPDL
                     LRAGGTISGPALMALADTAAYFVTLAHAGPVASAATANLDIHFLVRPKPEPITAVATM
                     LRLGRRLSVTKVDLCSGDLLVAHATVTYALPSSK"
     gene            complement(9073..>9564)
                     /locus_tag="VMZ53_26895"
     CDS             complement(9073..>9564)
                     /locus_tag="VMZ53_26895"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HUS32168.1"
                     /translation="LGEVIGSHDGKAFRSFAQSLTLDALLAVANSHLEELAPRYQLER
                     VPKHDLELQVIDRDLGDEIRSVQSLSGGESFLVSLALALGLSSMSAHDVRVRTLLIDE
                     GFGTLDPATLDTALAVLDELQSTGRQVGVISHVPALVERVRAHVRVSPKGGGRSEVVI
                     AEA"
BASE COUNT         1644 a         3073 c         3215 g         1632 t
ORIGIN      
        1 gaggtacgaa cgtcgatcga caccgagcgc gtcgagcgcg agcgtctgct tcgtcaggcc
       61 catcgagccg agccacgcat tcgtggcgct gcccgatgcc gcgacaaaca gcagcgccga
      121 gagcgcaggt gcgagcgcga cgacgtgact gccgccgatc tggcgcagca acgcttcggg
      181 ctttacgcct gcaccgccga ccttggagat cacgaacagc accgtgtagc cgatcagcgt
      241 cgagacgatc gcgtagaacg gcagcggccg aagcagtgtc tgcttgacga ctcgccagct
      301 cacgatcgcg aagtcgcgag gatgtcgcac gagctgcacc ggcgctttcc agagcgacag
      361 cgccgcgacg cgaaacggcg cgagcaacgg atcgacgagc cggcccatcg aacgcaacgc
      421 gcctcgcctc ggaggagcac ccgcgggcgg cgcgcgatcg ccgtgctcct cgagatgctt
      481 caccagcacg tcttcgagct cgacgagcca gcggccgcgc tcttctgccg aatgtcccgg
      541 atcttccagc gcacccggcc agcgatcggc aaacagctgc tcgagcttgc gatcggcgag
      601 cgacagcatg aacagcttgt cagcgacgcc cgcggcgaga gccacgtcgt gcgtcaccac
      661 gatcgcggag atgccgagcg ctttgacttg ctcgcggatc agccgcgcaa gcacgcgcac
      721 gcgatgtgga tcgagaccga cggacggctc gtccaagaag agcagcttgc gcttactcgc
      781 gagcacgcgc gcgacagcaa cgcgttgcgc ttgcccgccg gacagcgcgg tgaccggcgt
      841 acccggttct gcgagctcgc gtgagaggcc aacgcgctcg agccagtctt ccgccttttc
      901 atcactgcgt gcgtggcgaa gcgcgagctc gaggttgccg cggacgtcga gatgatcgaa
      961 cagcgcgcct cgctgtggca cgaggccgag ctggccgcga tcgagatcga gctgcgcggc
     1021 gaccacatcg aaacccgcag cggcgagctc gtctcgttcg aagagcacgc gcgcgaacgt
     1081 cgatttaccg gcgcccgatc cgccgagcag cacgatgact tcgccttcgc cgatcgagag
     1141 atcgattccg tcgatgagca cgcggccgtc ggggagcgcg acgcgtaagc cgcggacagc
     1201 gagtgcaccc atctgtgtag gcgaaacggt accagacacc agcggttgga atgcgttgca
     1261 gcgatttcgc aagcaaaaaa tcaggaaccg ccacgcacgg ccgatgtctc aatcgctgaa
     1321 ccgcaacgac tcactgaagc gcttggtagc gcgaggtggg aggcggccgg acggaggtct
     1381 atctacatgt ctgcgcaaca ggatttcttt ggtgaaaagg cagttgaagt agcaacccta
     1441 ctcggcgact cggtcgtcga tgtgaagcac tgcatggacc cacatggcgg caaggtcacg
     1501 ccgcgtacat gggccatgtt ggcaaccggt gcagcgtgtc tgctcctgag tgcaggtgcg
     1561 ttcatcgtgt cggtgaagac cgccgcgtac aaccacggcg cgcaggacta ctggacgcac
     1621 gtcgcgcata agccgcaggg cgcgttccgc ggtgtcgtgc tgtccggcgc gtatgactgg
     1681 ctcgccttcg gcggcttcct gctcggcctg ctgacggtcg cggtgggtct ctatcgcgct
     1741 cgcaaggaga agcaggaccc gacgtacaag atcggaacgg cgtctgacgt gcgcgtcgca
     1801 gtcgaaggcg caccggcacc gagcttcgcg atggtcgcgc cgaagggcga tgacttcgtc
     1861 ttcaactacg gcgtcggcat gaccggcgag atgctcatcg atggcaagac gattccgctc
     1921 gccgagctcg ccgcatcggg acacgcacgt ccatcgacgt ctgctgtcgg tgcattcgag
     1981 ctgccgattc cgatgaacgg ccgcattcgc gccaacatcg gcctgacgac gttccaggtc
     2041 tccgcggtcg cgaagccgca ggcgcagtcg tcgccgatgt tcgcgatgtc gagccgcacg
     2101 ctgtcgtact tcgcgggctc gctcggcgcg cacctcgccg ccgtgctgct gctcgcgcag
     2161 atcccgatgg atgacggctc ggcgtcgttc gatctcgccg cgctcgagcc gacggacatc
     2221 cgctcgaaca acgggatcaa cgaggacgtt cctccgacgc cgcccgaaga cgtcgcgaac
     2281 aacacgggcg atggcaagga agcttcgggc aagtcgatgc agctcgaaga gggccaagct
     2341 ggcacgacga agtcgaacaa ccagcagagc cacatccgca tcgcgaacaa caatcgcgag
     2401 gacacgttcg cgcagcgcca agtcgaaatc gagaacgctc gccaggcagg catcctcggt
     2461 tcggccgcac tgcaagacgg ctcgctgttc gcggacctcg cgtcgcagag tgaaatcgcg
     2521 agtggcccgg actcgtcgaa cgtgtggggc gcgatctacg gcgccgacgg tgaaggctac
     2581 ggcacgttcg gttacggccg tcatggcttc ggtcccggtg gcggttgcta cggcacggac
     2641 tgcggcctga tcggcacgcc gaacggctac ggcaagattg gcctcggcaa gttcccgggc
     2701 agcggttggg acggtggcgg tggtggccct ccgatgcgtc gccgccacac gccgacggtc
     2761 ccgggcccgg tggttggtga ggcgaagatc gtcggtggtc tcgataaggc gatcatccgc
     2821 cgctacatca agcgcaacat cgacaagatc agctactgct acgagaagca gctcctcgcc
     2881 aacccgagca tcgaaggcac cgtgaagatc cagttcttca tctcgccgac cggcgcggtg
     2941 acgtcgtcga cgggtgaagg cttcaacggc gcggttgcga gctgtgtggc cggtgtcgtc
     3001 agcgcgatcg agttccctcg ccccgacggc ggtggcggtg tcacggtgaa ttacccgttc
     3061 acgttccacg cgtcgggttc cggttcgtaa cactccgtcc tcctccgtcc gcagacagcg
     3121 cccgcacgcc tcaccggttt gcgggcgttt ctgcgtgtac gatgcagctg tgagcctcga
     3181 agagttcggc tcgtacgtcg tgctggagca gctcggcgtt ggcggcatgg cctcggtgca
     3241 tgtcgccgag tcgcgatcga tgggcggctt tcgcaagcgc gttgcgctca agcgcctgct
     3301 gccgcacgcc gccgagaatc ccgacctcgt gcggtcattc ctcgacgaag cgcggctcaa
     3361 cacatacttg aagcacccga acgtcgcgca gatctacgac ttcggccaag tcggcgacgt
     3421 gtacttcatg gcgatggagc tcgtcgccgg cccgacgctg aaccagctcg cgcggcagtg
     3481 cgattcgacc gtcggaatca tcccgtttcc gatcacgatg aacatcctga tccaggtcct
     3541 cgacgcgctg gactacgcgc acaacctgcg cgacgagcac ggcaagcgcc tcggcatcat
     3601 tcaccgcgac gtctcgccac cgaacatcgt cgtgtcgaat cagggcctcg tgaagctgat
     3661 cgacttcggc atcgcaaaag cggcgagctc gtcggtgcag acgcaagtcg gcacggtgaa
     3721 gggcaagttc agctacatgg cgcccgagta cctgcgcgag aagctcgatc accgcgtcga
     3781 catctgggcc gtcggcgtga tcgcgcacga gctgctgacg aatcgccgcc tgttcgacgc
     3841 gaacgacgac ttcaaagtga tcgatatggt caaagagctg cgcatcgatc cgccatcgcg
     3901 gcgcaacgtc gacgtgccgc cggatctcga tgcagtcgtg atgaccgcgc tgcagcgcga
     3961 tcccgatcag cgctggcaat cggccgccgc aatgcggacc gcactcgcga atgcagcggc
     4021 ggagctgcag accgttgtga cgaacgcgca gctcatcgag tgggtcgagt ggacgttctc
     4081 gcagaagccg cccggcgaag gcagcgacct ctcgcagctg atccagatcc tcgagacgcc
     4141 gtcgcggccg tccgcacgcc tgtcgaatcc cgacgagccg acggtaaaag gccgcaagca
     4201 gagcgcacag ccggtgaaga gcgagcgcta cttcgctcgc gcgctgaacg cgttctcgac
     4261 gccgaaggcc ggcatcgcgg cggtgaagcg cgcattctcg acaccgcaag tcggcgctgc
     4321 gatggtgaag cgcaacagct cgggactgcg ctttgtgacg acgctgctgt tcttgctgct
     4381 gctcggggcg atcggcgtcg gcatcgcagc gtggtttgcg agctggccgt tcgatctaca
     4441 gcagctgcta ctcggctgag ctacttgccg aggacgcggc actgcgccga tgcagtgccc
     4501 caatcgccgg ccttgagcgt gagcacctcg agcttcggat ggtacttcac gacgcattcg
     4561 aagcagtcgt cgaagaccac ttggtagctg cccgcaggca cgccgagcga gtactgcccc
     4621 ttcgcgttca cttgcgctgt atgggtctta tgcgtcttgg cgtcgatcgc gacgaccttg
     4681 cacgcggcaa cattcattgc gtggccttcg tggccaggaa cgcccgaaac gaagcccttc
     4741 gagacctggc ctgagagcat cgtcttcttg ccgcccgcac ccggcgagac attggagctc
     4801 aaatcgcagc tcatgctgcc agtgccttcg cacggtcgct gattgggcgg cagcttcgcg
     4861 agcgcatcgg ctgcgcgcgc attgcccatt gcggccagca tcggccactg ctcgggatcc
     4921 tcgggcaggg gatcgatgcg cgcgatgccc gcgtcggcga tcgccggcga agcaacagcg
     4981 ggcgcgtcga cagcagcatc gatcggcagg ctcggcgcgg tggacacgct cgcgacggac
     5041 gcgtccggcg gcaccgcgcg gggctgttgc gctggcgtct cgcgtcgagc cgggcctgca
     5101 cacgcgacga gagcgaggac gagcgacgtg cgcatcgcgc gagtatagcg tcgtcgtggc
     5161 aggtcgcgct ctgacgctca gcgcttgtac ttgatcgcca tcacccactt gcgccacttc
     5221 ggctcgaact tctcgaggtc ttcgccgagc acggcggcga gcgcggtggc accggtgaga
     5281 tcctttttgt cctcgagaaa cttcttgtag aactcctgca gctttccctg ctcctgcagg
     5341 tagtagacga ggtagcgcgc gtacgcgtac gagtcgtagt cggcttcgta gaactgatcg
     5401 cgcgtcgtgc cgagcaactt cgatagcttt ggcagcgtgc ctgctttgat ctccttcttg
     5461 agattgggca ggcgccagtt cgggaagccg acgatgtggc ctttgacctc gccaggctgc
     5521 tcgtagagcg acgcgaggcc ttcgttgaac cacgacggca cgtcggggaa gtttgcctcc
     5581 atgtatggat gcacgagctc gtgcgacagc gtgccgaggc ccgacgcgtt catgatgaga
     5641 gcgttggcgt cgggcgagta gtagccgtac ggcgtgtcgg gcgtgtcgcc gaagtatttt
     5701 ttcgcgcctg cgcggaacga cttctcgttg tgaaacagcc acacctcgag gacgcggtcg
     5761 gggcgcttcg cgaagaagtc cttctcgagg agcgcgacct tgctgcggat gaagccggtc
     5821 gtgatcttct tgacgtactt gtcgccggag tcgccgacga cgacgaacgg cgcttcgacg
     5881 aggaccgtga agccttggcc tttgagcttt ttctcgagct cggcggcgcg cgcgctcagc
     5941 tccttttcgg tcggctcggc gtgagcgagc ggcgcgcggc cgaggtggaa cagcaggacg
     6001 gctgcggcga cggcgatcgc gatggtgccc ttcatcattg ccttgctaga cagctgaatt
     6061 gactcgttgt tgcgacccaa agatcgtcaa acttccggtg tcgtatacga tctagcgcat
     6121 ggtttctgtt ccgtggatcg cgggacttct cgtcgtcgcg accctcgtga actggctgcg
     6181 caagcggagc tgggccgatc gggtgaaggg cccggggctg tacgcggcag ttggagcggg
     6241 cgcggggctg gtggcgctcg tggttgcgct gctgatcgga acacccgtgg tcgaggggct
     6301 gacggatcag gcggtgcagt ggtcgacgta cccggtcgtg cgcggcagcg tgacgacatt
     6361 tgtgatggtc gcgatcgtga tcgccgccgg atcgctggcg tgcgagctcg tgctgcgcgg
     6421 ctttttggtc gagctcggtc gcgaattcac gcggagtaat gccgtggcgg tcctgatggg
     6481 cggcttcgcc gaggcgttgc tcgcggacgg cgacgccggg atgaagctcg gcgcgggcgt
     6541 gttcggcgtg ggcctcggcg cgatctacat cgcgagcggt cgcagtgtcg ttgcgcctct
     6601 gtgtgcgcgg ctcgtgttcc tgctcggcgc gctcgtgctc gaagcgctgc gcgttgtcgg
     6661 ctagagtgcc cgccatgagc gtcacggttc gcgaagcaac gccggccgat gcagcggtcc
     6721 tcatcgcgca tctgcaggag ctcgtaggcg agcctgggat caatatcccg ctcgcgcccg
     6781 acgaagtgac gatgaccgtc gagcaggaga aagcgatcct cgcagacttt gccgcatcgc
     6841 cgcgtgcgtt gatgctcgtc gctacgggca cggagggcgg tgtgtcgcag atcgtgggcg
     6901 agctctcgct caaggcgatc tcgtcgcgca aggcggtgca gcacgtcgcg acgctcggca
     6961 tgtcggtgaa gcaggggtgg cggcgcaagg gcgtcggacg ggagctcatc accgccgcgc
     7021 tcgagtgggc gccgagcgca ggcatcaagc gcgtcgagct gtatgtctac gcgcgcaacg
     7081 tgcccgcgat cgcgctctac gagaagtgtg ggttcgcgat cgaaggccgg cgcaccgcgt
     7141 tcattcgcga aggcgagacg ttcctcgacg acctcgtgat ggcgaaaatc ctctagccga
     7201 aaaggaaaac cgccgcgcga cggttaagcc gcgtggcggt ttgtagcgcg tactcttact
     7261 gcttagcggt tcgtcgaacc gccgccgtag ccgtagccgc tgcttccgta gccggtcacg
     7321 tcagccgcgc cgcccgagaa gctctgctgc gacgaaacgt ccgtcgcgct cgagaccttc
     7381 gaggccgcgt catggagacg cgattcagcc tgggtgcgga gatccgccgc cttgttcttg
     7441 gcgccggaga tcaggtcagt ccagctctgc ttgatgctgg cgaccgtgaa ttcaccaccg
     7501 cgcttgcggt gcgcgtacat gagaccaccg ataccagcga gagcgacgag tttgcggaaa
     7561 gtcatgacta aattcctcct tgttctgtat gaacaagagg caatcgccgt gcctcgcgct
     7621 gtgcgccgtt gcccgagtgc gcccgcgtct cacagcatgc ggatcgcctc cggaatcgtg
     7681 catccgtgac ggcgccgcgt cgcgtgagga gccgtcggcg gcagaagcgc cgttcgctcg
     7741 aacgccaacg accgcgacgt cagggcgcag cgcgcgcgtg gcgatgagcg cactgacggc
     7801 tcgagccgcg ataccatcgt cgcgtggaag tgttcttcct catgatgggc ctgtggctcg
     7861 tcgtcgcggt ggctctcggc atccgacgcc cgcgtggcct cggcggagcc gcacttcgcg
     7921 cgcgcctcga cgccgtgtac ggcgagccgc acgagctcgt ccaagtctcg ccgggaggat
     7981 tccccgaagc cgacatcgcg ttctacgatc gcgtgaagag cgcgctcgaa caaaagggct
     8041 actcgacgct cggcgacgtg gaggacctca cgctctcgcg catctacccg cacaaccgga
     8101 cgttcctgcg cctgttcgtc gacgcgggcc gcatgatccg cgcgagcgcc taccacctgc
     8161 atccgcgcgg cgtcgtgatc tcgctgctgc agctcgtcca actctggccg cgtcacctcc
     8221 gcgtcgtcga gctcgtcagc gaagtcggcg gcaagttcct cgtcacgtcg aacacgcacg
     8281 gcgtcgaccg gctcgagcca ccgcccgaag caaagatcga acgcctccca ctcgagacga
     8341 gcttcctcga agtcgtcgcg cgccacgaag cccgcatcac cgcctacctc aaagagcacc
     8401 ccgagcgcgc gcccacgagc ttcgaacgcc tcgacgacgt gctcgcgtcg atcgcgcgcg
     8461 cgcacctcgc gatggcgcga catcgccaaa aggtcggcgg cctctcgcgc gacgagctcg
     8521 agcgcctgaa aggccgcccc ctcagcgcca ccgaagaagc attcctgcgc gaagtccaag
     8581 caggcaagac cgaaccgagc tgacagcgcg ctttcgtagg ccgcgcgact acttcgacga
     8641 cggcaacgcg tacgtgacgg tcgcgtgcgc gacgaggaga tcgccggagc acaggtcgac
     8701 cttcgtcacc gacagccggc ggccgagccg caacatcgtg gcgaccgcgg tgatcggctc
     8761 gggcttcggg cgcacgagga agtgaatgtc gaggttcgcg gtcgcggcgc tggcgaccgg
     8821 gcccgcgtga gcgagcgtga cgaaatacgc cgcggtatcg gcgagcgcca ttagcgcggg
     8881 acccgagatc gtgccgcccg cgcgcaacaa gtcggggcga aacggcatcg tcagcgtgat
     8941 gtcgttgtcg ccgagcgccg tgatcgccga gaactttcgc cactcgggaa agccggtgtc
     9001 catgagtttc tcgatctcgg cgatcgtcat ccgcggcatg tcgcgcagct tcgcatgatc
     9061 ggctcgcgtc gatcaggctt cggcgatgac gacttcgctg cggccgccgc ctttgggcga
     9121 gacgcggacg tgcgcgcgga cgcgttcgac gagcgcgggg acgtgcgaga tcacgccgac
     9181 ctggcggccg gtggactgca gctcgtcgag gactgcgagc gctgtatcga gcgttgcggg
     9241 atcgagcgtg ccgaagcctt cgtcgatgag gagcgtgcgc acgcgaacgt catgcgccga
     9301 catcgaggag aggccgaggg cgagggcgag cgagacgagg aatgattcgc cgccggacag
     9361 cgattggacc gaacggatct cgtcgccgag gtcgcggtcg atgacttgca gctcgaggtc
     9421 gtgcttcggg acgcgctcga gttggtagcg cggcgcgagc tcttcgaggt gcgagttcgc
     9481 gacggcgagc agggcgtcga gcgtgagcga ttgcgcgaag ctgcggaacg ccttgccgtc
     9541 gtgcgagccg atcacttcgc cgag
//
LOCUS       DATLBZ010000537         6957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3345977, whole genome shotgun sequence.
ACCESSION   DATLBZ010000537 DATLBZ010000000
VERSION     DATLBZ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3345977"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VMZ53_26900"
     CDS             <1..1225
                     /locus_tag="VMZ53_26900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32169.1"
                     /translation="VVVGAINQTSVWEPAKAQPKPEPTPANDPWAVPTTWVSPTPAKM
                     SQPSDTSLIDGFAYGNGVVVSGMGVSLVVSTQLSTRYLGWKQLAAGNLANAGDDIVMS
                     LSGSRFVWLDASLDVKRDIELHDSPNGPWIYGIPVGERHVVTQTSRDGKYEISMTDVD
                     GKTSTKPLTYTDAERLEYSPASRLFAVGMRRKIDRYKLDLGTNEMSPLPQLRVQGSPI
                     TIRLYDPAVAHGMTAAVISWDSDYADYQSLTFYREKGKPTRIHPFDGQILSEDADGTL
                     YVYSRLGTPELRVMKNGKVERKLKLEGTSIPAITKDASRVAILDGKGAIVVSDGTTGT
                     ELWRQPMWGAQQVLFSGDGKKLVVRAIGGIATFDSTTGIRTGLECGWNFGIYDEPLGS
                     SPPGQSMVCEDPMLQ"
     gene            1285..3318
                     /locus_tag="VMZ53_26905"
     CDS             1285..3318
                     /locus_tag="VMZ53_26905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32170.1"
                     /translation="MCLTPSCSAPPTPHPAKTPAKPLPPQPPAEQLPGAPVATAHKLR
                     DAPEAPHGGFILQIVTSLDGAAAVSVDELGGVRLWPTLDGTREPRVIELPQAKQLALA
                     RGPNDGFQIIALDEVGGLYFAKLDNQARTLSHATLPPEPAFAGIAMTELGLLAWRVDQ
                     TIVLLDADGATKARIATKPKERVVAIAAANKRAIAITEIEDGSRSVRWLDIDPKLAWG
                     GEIYLGGDPGQDIALSPKHTYYAIARREERSTLVQVVDVAKKKVVASTNINMQGVDLA
                     FTDDTTLAIGTFEGISWVDTTLPMPVMSQVTRTSGSRVAPTLAAANGKAITTQNGELV
                     LATPSTTQFLGYDLVAPRFVEPAGNGQLLVGVKEAFTLIGANLRETPGNPGSWASIAL
                     PTNTMTQVLWLGGSDWLVETAAGGATPITLSVVDVAKNVTTPLRPNLREANVLMYEPS
                     TNIVTLSFGAEAEVAKWDAKSRQLTKLASITKPSPYEQELLAPLNPKLANELQLVHVV
                     MRDKPTIKWLRDPRSLATAASQITIIDGSYAGADAAGHVFVWRNSPSSKLELAVYADG
                     KPIAQLPAQGPVSLWPDPDGKRVIEIAANSVGLYQTTDGKQLWTQELSAVQEALWLSD
                     GSLAITSAGGIARLDPANGSITASRCGWRFGLTSAPHPQTSRIEPLCAQRAIQ"
     gene            3323..3661
                     /locus_tag="VMZ53_26910"
     CDS             3323..3661
                     /locus_tag="VMZ53_26910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32171.1"
                     /translation="MRLIWTLAIACVIAATGVRPQLEVRQDHTEQLQRATPGTLQHLT
                     ARSAVHHFAMRPHTLGTPDARSAPLAILPSAEFSTTPPRVRALSTAWSLHVSLSQLVP
                     AHPARAPPLG"
     gene            4051..6159
                     /locus_tag="VMZ53_26915"
     CDS             4051..6159
                     /locus_tag="VMZ53_26915"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HUS32172.1"
                     /translation="MTGAHVLSELVIVLGTAAVITLIFQALRLPVVLGYIAAGLVIGP
                     HVPVPLVANVHLIATLSELGVILLMFALGLELRISTLARVGIGAGLTALFEVALALTA
                     SALAARALGASGPESLFYGACFAISSTMLVAKAFEEHGWKGGFTDVVFAILVFEDLIA
                     IALLAVLTGVASGSGLGPKELAVTLGKLAGFLALVLVVGLLIVPRFMRLVAKLKRSET
                     LLIAALVICFGLAVLTEYVHYPVALGAFVAGLLVAESGLGHEVSDLVRPFRDVFAMMF
                     FVSIGMTIEPAQLASNIPRILLFTALVLVMKPLGIALASFTAGRGLQPAIRSGVSLAQ
                     TGELSFVIAGIGLAAGVASPDLLAIAVGVTCLTTLTSSVMIGRSEAIARTASARIPSR
                     VATFVSFYESWLGRLRGRERLTWLRVRRPVFVLVADVIAITALIIAGSFAHRYVATEV
                     GFSKLGAKIIVAGLVLAVISPFLVGLIRRVVVIARALAYTIIPPPSESGVVVDHQAAN
                     VVGLGAAAEALDLGRSARRALILTLELAIAAAISLPMIAAIQPFIGTTGLVALTVLLV
                     LLAVAYRSIVDFTQHVRAGSELILELLHHPVAPPTEAAAATEQPLSQIESILPGFGGL
                     VTVTLDPSSPAVARSLADLDLRARTGASVLAIARGEGGFATPKPTEPLQPGDILALAG
                     SDEAITAARAALTTAPPSTA"
     gene            complement(6164..6622)
                     /locus_tag="VMZ53_26920"
     CDS             complement(6164..6622)
                     /locus_tag="VMZ53_26920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32173.1"
                     /translation="MKWLVVLCAMTTPAFAERPVHGSVGGGTSFLLTGAQGDRNRFEL
                     EVDVEPPRSRFGGLLAWRALDDAHNGMLLAGVVYEAGAARPRLVVDLHADIGAELDQK
                     APVAGGGIRTTLTIWKMFGIALDGGAYLVIDGVEDTRLVFAGSSSLVLRW"
     gene            complement(6619..>6957)
                     /locus_tag="VMZ53_26925"
     CDS             complement(6619..>6957)
                     /locus_tag="VMZ53_26925"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HUS32174.1"
                     /translation="HLRRQITARWAPRGQLSVFTVDAMIEDAVRGAIDRRESGVQVLA
                     LEPAIAQDIVAAVRGKVGDKPAVILTSGDVRRHLRTLLEPELPDVAILAAHELAPGTS
                     VKTAGRIDVA"
BASE COUNT         1173 a         2656 c         2017 g         1111 t
ORIGIN      
        1 cgtcgtcgtc ggcgccatca accagacatc ggtctgggag cccgcgaagg cgcaacccaa
       61 gcccgagccg acgccggcaa acgatccgtg ggccgtcccc acgacgtggg tgagcccgac
      121 gcccgcgaag atgagccagc cgagcgacac gagcctgatc gacggctttg catacggcaa
      181 tggcgtcgtc gtcagcggca tgggcgtctc cctcgtcgtc agcacgcagc tctcgacgcg
      241 ctacctcggc tggaagcagc tcgccgcggg aaatctcgcg aacgccggcg atgacatcgt
      301 catgagcctg tcaggcagcc gcttcgtctg gctcgatgcc agcctcgacg tcaagcgcga
      361 catcgagctc cacgacagcc cgaacggacc gtggatctac ggcatccccg tcggtgagcg
      421 ccacgtcgtc acgcagacct cgcgcgacgg caaatacgaa atctcgatga ccgacgtcga
      481 cggcaagacc agcacgaagc cgctcacgta caccgacgcc gagcgcctcg agtactcgcc
      541 cgcatcgcgc ctgttcgccg tcggcatgcg ccgcaagatc gatcgctaca agctcgacct
      601 cggcacgaac gagatgtcgc cgctgccgca gctgcgcgtg cagggctcgc cgatcacgat
      661 tcgcctctac gatcccgcgg tcgcacacgg catgaccgcc gccgtcatca gctgggacag
      721 cgactacgcc gattaccaat cgctcacgtt ctatcgcgag aagggcaagc cgacgcgcat
      781 ccacccgttc gacggccaga tcctctccga agatgccgac ggcacgctct acgtctactc
      841 gcgcctcggc acgcccgagc ttcgcgtgat gaagaacggc aaggtcgagc gcaagctgaa
      901 gctcgagggc accagcatcc cggcgatcac gaaagacgcg agccgcgtcg cgatcctcga
      961 cggtaaaggc gcgatcgttg tcagtgacgg cacgaccggc accgagctgt ggcgccagcc
     1021 gatgtggggc gcgcagcagg tcctcttcag cggcgacggc aagaagctcg tcgtgcgcgc
     1081 gatcggtggc atcgcaacgt tcgattcgac gaccggcatc cgcacgggcc tcgagtgcgg
     1141 ctggaacttc ggcatctacg acgaaccgct cggctcctcg ccgcccggcc aatcgatggt
     1201 gtgcgaagat ccaatgctcc agtgatgggg tcaggcacaa gttcacaagt ttctaggctc
     1261 gctcacgccg cgttacttgt ttacttgtgc ctgaccccct cgtgcagcgc accaccgacg
     1321 ccgcatcccg cgaagacacc cgcgaagccg ctacctccgc agccgcctgc cgagcagctt
     1381 ccgggcgcac ctgtcgccac cgctcataag ctgcgcgacg caccggaggc tccgcacggc
     1441 ggtttcatcc tgcagatcgt gacgagcctc gacggcgcgg ccgcggtgag cgtcgacgag
     1501 ctcggcggcg tgcggctctg gcccacgctc gacggcacgc gagagccgcg cgtgatcgag
     1561 ctaccgcagg ccaagcagct cgcactcgcg cgcggtccga acgacggctt ccagatcatc
     1621 gccctcgacg aagtcggcgg cctctacttc gcgaagctcg ataaccaagc acgcacgctc
     1681 tcgcacgcga cgcttccacc ggagcccgcg ttcgcaggaa ttgcgatgac cgagctcggc
     1741 ctcctcgcgt ggcgcgtcga ccagaccatc gtcctcctcg acgccgacgg cgcgacgaag
     1801 gcacgcatcg cgaccaagcc gaaggagcgc gtcgtcgcca tcgcggctgc aaacaagcgc
     1861 gcgatcgcga tcaccgaaat cgaggacggc agccgcagcg tccgctggct cgacatcgat
     1921 ccgaagctcg catggggcgg cgagatctat ctcggcggcg atccaggtca agacatagcg
     1981 ctctcaccga agcacacgta ctacgcgatc gcgcggcgcg aagaacgcag cacgctcgtc
     2041 caggtcgtcg acgtcgcgaa gaagaaggtc gtcgcgtcga cgaacatcaa catgcagggc
     2101 gtcgacctcg cattcaccga cgacacgacg ctcgccatcg gcaccttcga aggcatcagc
     2161 tgggtcgaca ccacgctgcc gatgcccgtc atgtcgcaag tcacgcgaac gagcggcagc
     2221 cgcgtcgcac cgacactcgc cgccgcaaac ggcaaggcga tcacgacgca gaacggcgag
     2281 ctcgtgcttg caacaccgtc gacgacgcaa tttctcggct acgacctcgt cgcgccgcgc
     2341 ttcgtcgagc cggcaggcaa cggccagctc ctcgtcggcg tgaaagaagc gttcacgctc
     2401 atcggcgcga acctccgcga gacgccgggc aaccccggca gctgggcgtc gatcgcattg
     2461 cccacgaaca cgatgacgca agtcctctgg ctcggcggca gcgattggct cgtcgaaacc
     2521 gccgccggcg gtgccacacc gatcacgctc tccgtcgtcg acgtcgcgaa gaacgtcacc
     2581 acgccgctgc gcccgaacct cagagaagcc aacgtgctca tgtacgagcc gagcactaac
     2641 atcgtcacgc tctcgttcgg cgccgaagcc gaggtcgcga agtgggacgc caaatcgcgc
     2701 cagctcacga agctcgcgtc gatcacgaag ccaagcccct acgagcaaga gctcctcgcg
     2761 ccgctcaatc ccaagctcgc gaacgagctg cagctcgtcc acgtcgtgat gcgcgacaag
     2821 ccgacgatca agtggctgcg cgatccgcgc tcgctcgcca cagccgcgtc gcagatcacg
     2881 atcatcgacg gctcgtacgc cggtgccgac gctgcaggcc acgtcttcgt ctggcgcaac
     2941 tcgcccagca gcaagctcga gctcgcggtc tacgcggacg gcaagccgat cgcgcagctc
     3001 cccgcacaag gcccggtgtc gctgtggcct gaccccgacg gcaagcgcgt catcgagatc
     3061 gccgccaatt cggtcggcct ttatcaaacc accgacggca agcaactctg gacgcaggag
     3121 ctctctgccg tgcaagaagc tctgtggctc agcgacggct cactcgcgat cacgagcgcg
     3181 ggtgggatcg cgcgtctcga ccccgcgaac ggctccatca ccgcctctcg ctgcggctgg
     3241 cgattcggtc tgacctctgc gccgcatccg cagacatctc ggatcgagcc actctgcgcg
     3301 cagcgtgcta tccaataacg ccatgcgttt gatctggacc cttgcgatcg cgtgcgtcat
     3361 cgccgcgacc ggagtgcgtc cgcagctcga ggtccgccag gaccacaccg agcagctcca
     3421 gcgcgcgacg ccgggaacgc tccagcacct cacggcgcgt tcggccgtcc accacttcgc
     3481 gatgcgcccg cacacgctcg gcacgcccga cgcccgcagc gcaccgctcg cgatcctgcc
     3541 atccgcagaa ttctcgacga cacctccgcg cgttcgcgcg ctctctaccg cgtggtcact
     3601 ccacgtctcg ctctcgcagc tcgttccggc gcatcccgca cgtgctccgc cgctcggctg
     3661 aacgctccaa cccgttcacc gatttgcgac cacgtcaccg cctgtcacgg cggaaaggtt
     3721 tcctatgcgt ccggaatctg atcacgatga cgatatttcc tccactccga cgagctttgg
     3781 gcagcgccac ggcgtaacga tcatgacggt cgtgctcgcc gggctcctca tccttgtctg
     3841 ctgcgtgcag gtggcctgct gatggccggc ccgatcgcac ttgcagcggc actcgcgcgc
     3901 accgtcccga ttcggtgccc gtggtgcggc catcgcaagc tcgtggctcg taagcccgcg
     3961 gccgtcgcgt atcgggtgtg cgcgcgctgc aagcgccact tcccggatcc gctggccacc
     4021 aagccgagcc gcacgcgccg atagccaccg atgaccggcg cgcatgtcct cagcgagctg
     4081 gtgatcgtcc tcggcacggc cgcggtgatc accctcatct ttcaagccct gcgtttgccg
     4141 gtcgtcctcg gctacatcgc ggccggcctc gtcatcggcc cgcacgtgcc tgtcccgctc
     4201 gtcgcgaacg tgcacctgat cgccactctc tccgagctcg gcgtcatcct cctcatgttc
     4261 gcgctcggtc tcgagctgcg gatctccacg ctcgctcgcg tcggcatcgg cgccggcctc
     4321 accgcactct tcgaagtcgc gctcgcgctc accgcgtcgg cactcgcagc ccgcgcgctc
     4381 ggcgcatcag gccccgagtc gctcttctac ggtgcgtgct tcgcgatctc gagcacgatg
     4441 ctcgtcgcca aagcattcga agagcacggc tggaagggcg gcttcaccga cgttgtcttc
     4501 gcgatcctcg tgttcgaaga tctcatcgcg atcgcactcc tcgcggtcct caccggtgtc
     4561 gcgtcgggca gcggcctcgg acccaaggag ctcgcagtca cgctcggcaa gctcgccggc
     4621 ttcctcgcgc tcgtcctcgt cgtcggcctc ctcatcgtgc cgcggttcat gcgcctcgtc
     4681 gcgaagctca agcgatcaga gacgctcctc atcgcggcac tcgtcatctg cttcggcctc
     4741 gccgtcctca ccgagtacgt ccattacccg gtcgcactcg gagcattcgt cgccggcctc
     4801 ctcgtcgcag aatccggcct cggccacgaa gtcagcgatc tcgtccgccc gttccgcgac
     4861 gtcttcgcga tgatgttctt cgtctccatc ggcatgacga tcgagcccgc gcagctcgcg
     4921 tcgaacatcc cgcgcatcct cctgttcacc gccctcgtgc tcgtgatgaa gccgctcggc
     4981 atcgcgctcg catccttcac cgccggtcgc ggccttcaac cggcaatccg ctccggcgtc
     5041 agcctcgcgc aaaccggtga gctctcgttc gtcatcgcag gcatcggcct cgccgccggc
     5101 gtcgcaagcc cagacctcct ggcgatcgcg gtcggcgtca cgtgcctcac gacgctcacc
     5161 tcgtcggtca tgatcggccg ctccgaagcg atcgcgcgca ccgcctccgc acgcatccca
     5221 tcgcgtgtcg cgacattcgt ctcgttctac gagtcgtggc tcggccgcct gcgcggtcgc
     5281 gaacgcctca cctggctccg cgtccgtcgc cccgtcttcg tgctcgtcgc cgacgtcatc
     5341 gcgatcaccg cgctcatcat cgctggctcg ttcgcacacc gatacgtcgc gacggaggtc
     5401 ggcttctcga agctcggcgc gaagatcatc gtcgccggcc tcgtgctcgc cgtgatctcg
     5461 ccgttcctcg tcggcctcat ccgccgcgtc gtcgtgatcg ctcgcgcgct cgcctacacg
     5521 atcatcccgc cgccgtcaga gagcggagtc gtcgtcgatc accaagccgc caacgtcgtc
     5581 ggcctcggtg ccgccgccga agcactcgac ctcggccgct ccgcacgccg cgctctcatc
     5641 ctcacgctcg agctcgcgat cgcagccgcc atttcgctcc ccatgatcgc tgcgatccaa
     5701 ccgttcatcg gcacgacggg cctcgtcgcg ctcaccgtcc tcctcgtcct cctcgcagtc
     5761 gcctaccgct cgatcgtcga cttcacgcaa cacgtgcgcg caggcagcga gctcatcctc
     5821 gagctgctcc accatcccgt cgcgccgcca acagaagccg ccgccgcaac cgagcagccg
     5881 ctctcgcaga tcgaatcgat cctccccggc ttcggtggtc tcgtcaccgt cacgctcgac
     5941 ccatcatcgc cggccgtcgc acgctcgctc gccgacctcg acctccgcgc acgcacgggc
     6001 gcctccgtcc tcgcaatcgc acgcggcgaa ggcggcttcg cgacgcccaa acccaccgag
     6061 ccactacagc ccggcgacat cctcgcgctc gctggctccg acgaggccat cacggcagca
     6121 cgcgccgcgc tcacgactgc gccgccgtcg accgcgtgac aggtcaccag cgaaggacga
     6181 gcgacgagct tcccgcgaaa acgagtctcg tatcctcgac gccatcgatg accaagtacg
     6241 caccgccgtc gagcgcgatg ccgaacatct tccagatcgt cagcgtcgtg cgaatgccgc
     6301 cgcccgcaac cggcgccttc tgatcgagct ccgcgccaat gtcggcatga agatcgacga
     6361 cgagccgcgg ccgcgccgca cccgcctcgt acacgacccc tgccagcagc atgccgttgt
     6421 gcgcatcatc tagcgcgcgc cacgcgagta gcccgccgaa gcggctgcgc ggcggctcga
     6481 catccacctc gagctcgaag cgattgcgat cgccttgcgc gcccgtcagc agaaacgacg
     6541 tcccgccgcc tacgctgcca tgcacgggcc gctcggcaaa tgcgggcgtg gtcatcgcgc
     6601 agagaacaac gagccacttc acgcgacgtc gatgcgtcca gccgtcttca ccgacgtacc
     6661 aggcgccaat tcatgcgcgg ccagaatcgc gacatcgggc agctcgggct cgagcagcgt
     6721 tcgcaagtgc cgccgcacat caccgctcgt cagaatcaca gccggcttat cgccaacctt
     6781 gccgcgcacc gcggcgacga tgtcctgcgc gatcgccggc tcgagagcca atacctgcac
     6841 gccactctcg cgcctatcga tcgcaccgcg caccgcatct tcgatcatcg cgtcgacggt
     6901 aaacaccgac agctgaccgc gtggtgccca gcgcgccgtg atctgtcgac gcagatg
//
LOCUS       DATLBZ010000538         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1447689, whole genome shotgun sequence.
ACCESSION   DATLBZ010000538 DATLBZ010000000
VERSION     DATLBZ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447689"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VMZ53_26930"
     CDS             <1..244
                     /locus_tag="VMZ53_26930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32175.1"
                     /translation="GGTHRGLYDALAELAAHAAAAPAAAASAAPAGAASAATAVAAPH
                     APAAAAPDATPDVVPARHYIAKAVILAMGFELAPTS"
     gene            491..>2006
                     /locus_tag="VMZ53_26935"
     CDS             491..>2006
                     /locus_tag="VMZ53_26935"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS32176.1"
                     /translation="MLLVGLASASMFVLGVSDPSTARADAVLSRGNTQLFPTRIDVAV
                     EIRAQIESTTVVIELPPIETAGDYTLTVPTPAGASPVGVDIDRGAGFVALPVITGAPP
                     SASGGGTASAEYDAWAGTAPLGASLQALEPGPLTARVRFVRILRRVGGHVGFEVGSHR
                     CPLRAPYDPGAVATVRLSLATSRDLASFGATSATVDTSSASFNATARSAGFVAPPVAG
                     DAVVDVGYAEVSNGINVQLLTHRTPTADPLGGDAGYFLLLVDADTAPSTTPRAISLVI
                     DHSGSMAGDKIAQARDAARAMLDYLRPSDAFTIHIFDDDIDSFRSAPVPATPENIDAA
                     RSYIKGIEDSGSTNLNGGLQAALESPSSAERFDAVVMLSDGVATSGETDDRAIIANAW
                     RNAGDTRIFTFSIGSDADFPLMQALAQGSRGKHVDLNNVQATRDLVLRTRELFEDIRD
                     VRLTDLDMEVSNVTLTDTLPEQMPDLFSGGQVILVGRYTSSGSGSLTITGMEGAV"
BASE COUNT          315 a          690 c          678 g          323 t
ORIGIN      
        1 cggcggaacg catcgcggcc tctacgatgc gctcgcggag ctcgccgcgc acgcggctgc
       61 cgcgcccgca gctgcggcaa gcgccgcgcc cgcaggtgcc gcaagcgccg cgaccgcagt
      121 tgccgcgccg cacgcccccg cagctgccgc gccggacgcg acaccggatg tcgtacccgc
      181 gcgccactac atcgcgaagg ctgtgatctt ggcgatgggc ttcgagctcg caccgacgag
      241 ctgaacctcg atctcgtcga ggacgttcga ggtgttcggc tgtcgcaccc gggtggcgcg
      301 atcttcctgg ttcgttggcc ggtgacgccg tgtgcgctga ccgcggatga ccaatcgata
      361 tccgcgatcg cgtgcgacac atcgcggcag acgattcgca gcgctcgaag aggtgtgagc
      421 ggcgaatgcg ggcgtcctgc gcgcgattgc ttcagcgtgg atgcgggagg tcgagatgaa
      481 acgtgaacga atgctccttg tcgggctcgc gagcgcgtcg atgttcgtgc tcggtgttag
      541 cgatccgtcg acggcgcgtg cggatgctgt gctcagccgc ggcaacacgc agctgttccc
      601 gacgcgcatc gacgtcgcgg tcgagattcg cgcgcagatc gaatcgacga ccgtcgtgat
      661 cgagctcccg ccgatcgaga cggccggcga ctacacgctg accgtcccga cccccgcggg
      721 cgcgagcccc gtcggcgtcg atatcgatcg cggtgcgggc ttcgtggcgc tgccggtgat
      781 cacgggtgcg cccccgagcg catcgggtgg cgggacggcg agcgcggagt acgacgcgtg
      841 ggcgggcacc gccccactcg gtgcgtcgct gcaagcgctc gagcccggtc cgctgacggc
      901 gcgcgtgcga ttcgtgcgga tcctccggcg cgtcgggggc cacgtcggct ttgaagttgg
      961 atcgcatcgc tgcccgcttc gtgcgccgta cgacccgggc gccgtcgcga ccgtgaggct
     1021 ctcgctcgcc acgtcgcgcg acctcgcgag ctttggcgca acgagcgcga ccgtcgatac
     1081 gagcagcgcg tcgttcaacg cgacggcacg aagcgccggc tttgtagcac cgccagtcgc
     1141 cggcgatgca gtcgtcgacg ttggctacgc cgaggtctcg aatggcatca acgtgcagct
     1201 gctcactcat cgcacgccga cggccgatcc gctcggtggc gatgccggct acttccttct
     1261 gctcgtcgat gcggacactg caccgtcgac gacaccgcgc gcgatcagcc tcgtgatcga
     1321 tcactcgggc tcgatggcag gcgacaagat cgcgcaagcg cgagacgcag cgcgcgcgat
     1381 gctcgactac ctacggccat cggacgcgtt cacgatccac atcttcgacg atgacatcga
     1441 ctcgttccgc tcggcgcccg tgcccgcgac gcccgagaac atcgacgccg cgcggtcgta
     1501 catcaaaggc atcgaggaca gcggctcgac gaacttgaat ggcgggctgc aggctgcgct
     1561 cgagtcgccg tccagcgcgg agcgcttcga tgcagtcgtc atgttgtccg acggcgtcgc
     1621 gacatcgggc gagaccgacg atcgcgccat catcgcgaac gcgtggcgca acgcgggtga
     1681 cacgcgcatt tttacgttct cgatcggcag cgatgccgac ttcccgctga tgcaagcgct
     1741 cgcgcaaggc agccgcggca agcacgtcga cctcaacaac gtccaggcaa cgcgcgacct
     1801 cgtgctgcgc acgcgcgagc tgttcgaaga catccgcgac gtgcggctga cagacctcga
     1861 catggaggtc tcgaacgtca cgctgaccga tacgcttccc gagcagatgc ccgacctctt
     1921 cagcggtggt caggtgatcc tcgtcggtcg ctacacgtcg tcaggctccg gctcgctgac
     1981 gatcaccggc atggaaggtg cggtgc
//
LOCUS       DATLBZ010000539        18222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_708797, whole genome shotgun sequence.
ACCESSION   DATLBZ010000539 DATLBZ010000000
VERSION     DATLBZ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18222
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708797"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /locus_tag="VMZ53_26940"
     CDS             complement(<1..1968)
                     /locus_tag="VMZ53_26940"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUS32177.1"
                     /translation="MSAPSKITRRSFLVGLGLTTGGLAIGLRHAHAEEPSGKNGPTAP
                     PAPQGKRGLDPHALVHIDLDGSTTIFCHRSEMGQGVRSTIPVLIADELGADPARITVR
                     QAEGDKKYGDQDTDGSSSIRQQYESLRQAGAAARMMLCAAAAKKWGVKPETCTTINHE
                     VVHAKSKRKVPFAAVVADAAKLPVPKPKDIVLRPASELTRTSEPNLPLLDGQAIVTGT
                     ATYGADVRFPDMLIAVIARPPVLGGKVTKVDKQRALAIPGVKHVIELPAPKPPWEFQA
                     WGGVAVVADTTWAALKGRAALDITWDHGPHASASSKATRDEMLASVREGGKAVRNVGD
                     IDAAFSKAAKTVEADYVVPHLAHLTMEPPAAIVRPLQGRTVEVYAPSQNPQGAREDVA
                     KIFGIDAANVTVNVTLLGGGFGRKSKADFIAEAAHLAKQLGIPIRVQWTREDDIRHDY
                     YNAANAQHLRAALDAQGNVTAWHHRTALTPIATTFSDKPGADTPGLGDLQQGVVDVAL
                     AIPNVRAEPCKATPHVRVGWYRSVYNIFHAFGIGSFIDELAHARGKDPRDTWLEVIGP
                     AKKHSLADLGIPKLPNYGEPLDKHPVDAGRLRNVIERVTDAAKWSSRKGRFLGLAAHR
                     SFVTYTAVIISVVPDKTRVFRIDEAWITMDAG"
     gene            complement(1965..2435)
                     /locus_tag="VMZ53_26945"
     CDS             complement(1965..2435)
                     /locus_tag="VMZ53_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HUS32178.1"
                     /translation="MKLRINGKDHDLQQVDPEMPLLWALRDVIGLTGTKYGCGEALCG
                     SCTVHLDGHAVRSCVTPIRRAEGKAVTTIEGLSPDGSHPLQKAWLDLRVPQCGYCQAG
                     QIMSAAALLAVKPKPTDDEIDQSMAGNLCRCGTYTRIRAAIKKAAGTSDGGETK"
     gene            complement(2432..3085)
                     /locus_tag="VMZ53_26950"
     CDS             complement(2432..3085)
                     /locus_tag="VMZ53_26950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Isoquinoline 1-oxidoreductase subunit"
                     /protein_id="HUS32179.1"
                     /translation="MRRLALCLAVAACGGPKSAAAPSANPQLREAAAGELLAVRDFDA
                     IGDRAERSRALFGEMARVIMHPRCINCHPNGDTPHQGMQLALHDPPVVRGPDDHGVPA
                     MECTTCHQDRNQELTRVPGAPKWHVAPIEMAWVGKSAAYICNQIKDPKRNGGKSLAQL
                     VEHNGHDELVGWGWNPGADREKAPGTQQQFGELTAAWVETGAECPPEPIAAPTGETR"
     gene            complement(3220..3732)
                     /locus_tag="VMZ53_26955"
     CDS             complement(3220..3732)
                     /locus_tag="VMZ53_26955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32180.1"
                     /translation="MKRLLLLPLVFAAACSDQPTKEESVRVWSTASISLSNAQTQAVT
                     AAKTGGTGSTDVNLNYTGACTLGGSVAVTGNYAGEGDDEHAAFDLSASFDGCKDVSGS
                     LDGDIHWTSTADAAGFTAGMTGSLAWTSGSDSATCDFDLHLAVTQSAITYGGTLCGYT
                     MAELGLSVGH"
     gene            complement(3826..4962)
                     /locus_tag="VMZ53_26960"
     CDS             complement(3826..4962)
                     /locus_tag="VMZ53_26960"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HUS32181.1"
                     /translation="MLSIELEAALVRELRRAYERTNTKRFAGKLKPAVLVLGDTTKRL
                     GQWSRATRRLELSRTLVLERPWLEVISVLEHEMAHQFVDEVLGASAEPAHGPTFQRVC
                     AERGIDARAAGAPIAAADAEGERALDKIRKLMALAGSANEHEAEIAMRRAHELMLRHN
                     IEQARSGGANVYEVRQLGEPTRRANAVELDIVGLLTECFFVEVIRVPVYVAATASQAE
                     VFEVIGTRTNLDMALHVFEFMRATAQRLWEENRRDARVKSGRDRRAYQSGVVRGFREK
                     LVASRTSTSQGTSTTSQGTSTSQGASTSAGASEAVTSEALVWSGDDHLDAFFRARYPR
                     IRRRRRFMTMSSAHAAGREAGRTVILHKPVTRGPSDGSPRLLRD"
     gene            complement(5407..5667)
                     /locus_tag="VMZ53_26965"
     CDS             complement(5407..5667)
                     /locus_tag="VMZ53_26965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32182.1"
                     /translation="MTELVRCVCSRLNNAWRSYCGGCGSALQPRCDCGFVNGKSDRFC
                     GGCGVAMVSQLRASSGTMRPTNSNESTIPLDIIKDALSGEPL"
     gene            complement(5796..6395)
                     /locus_tag="VMZ53_26970"
     CDS             complement(5796..6395)
                     /locus_tag="VMZ53_26970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS32183.1"
                     /translation="MTAFDDELGNRRASEDDELGKRRTSEDAELLAHYIARGREPWNT
                     SLEASWLDYELRAFVIARLPTQRPLLVLNVGIGVGLFDDWLGHVIGGRITSIDRDADI
                     CRILELRQRREGHPHPARVICGDVRDGVLDGQSFDVITVVGSTLEESGDRGATTRALQ
                     SALTPTGQLLVAEVGQGAAGDRVLTCGDVWLACQTRVPA"
     gene            complement(6537..6959)
                     /locus_tag="VMZ53_26975"
     CDS             complement(6537..6959)
                     /locus_tag="VMZ53_26975"
                     /inference="COORDINATES: protein motif:HMM:NF022769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HUS32184.1"
                     /translation="MGIETTGKLHTIFDTKQVSERFSKREFVIELADNPKYPQTVLFQ
                     LTGDRCAQLDDLRVGDEVRIEFSLRGREWRSPSGEVKYFNSLDVWKIEPARAGRSARG
                     NGNGNGHGRGSGDPRDSEMPPRIDESQFGRVETDDIPF"
     gene            7008..7460
                     /locus_tag="VMZ53_26980"
     CDS             7008..7460
                     /locus_tag="VMZ53_26980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32185.1"
                     /translation="MQSLRDIAQAARRELMLCGVALEDAHTSLPSKLLALPPVVTAPM
                     RAWRLFRARRHRAAAKKHLDELHIALHERASLGVLNADLWSAIMRGVDALALSRWLAT
                     DELTAADVRPSRQTVQIILRDLSELIELLSELEGPGERASGSSPPSST"
     gene            7991..8545
                     /locus_tag="VMZ53_26985"
     CDS             7991..8545
                     /locus_tag="VMZ53_26985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32186.1"
                     /translation="MTLAVLGVGAAGAAGVWLKRWFRPEQRTRRLLRRTRVTPIKELT
                     DGQLACVVGTIVREGELIESLIERKRCVAFDTETNVFDGTGFSSPVRFETTRRMVPFY
                     VVDATGRVRVDAPQVALSNKPIAKGQSFVERVLVEGQRVRLVGSVVLDPTMAGNAEHH
                     FRETGGVHATLTGTAKFPLLADED"
     gene            complement(8542..11115)
                     /locus_tag="VMZ53_26990"
     CDS             complement(8542..11115)
                     /locus_tag="VMZ53_26990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS32187.1"
                     /translation="MAVTPDVVLRELAGLRALAQSLVHGDADAEDLLQETAIAAITHP
                     PTVEADGSPRPWLAKVLRNRWRMDRRARARRIAREQTSELELAATRGDASDVAGTATT
                     QHEAVDAVDAIDRARVLEKLAGALVALEEPFRTTIVRRYLDGDSAADIARALGVPAGT
                     VRWRLKTGLERLRASLDEKSPRWQRALAPFTAFKGAPAIASKASSTSTATTTSSLLKG
                     AVLVKAKTTIVSVIAVLLLLGGGGFAIYKLRGDGPSTSSPSIATSKAPGTGSAAGSGK
                     IIVEAAGSGETGSAAPEPVPGQGRVVVSTVAAEGGVISGRVVNWSTGEGVMDAEITFT
                     STAGATTIKSGKGGAFELAPPKPGTFALATILAPGFLPYAPELEHSPVRVALASKQAV
                     RGLTLFLQPALDYYGLVVDGSGAPVAGARVKLADPPSGEQSLEKVVNEWTTDKQGQFV
                     FHAQDETVFEATKGNLRGWAVLDGNAQTTHKLTIKIGNAPARDATIRGKVVDGSGAPV
                     PDVLVTAVPEDSPATAQRDAPRSVMFATSGNDGAFVLEGLDRKTYTLIGEDEDHAQAR
                     LDKIAGGTQNVTITLDAGLPLAGTVRNADDQPVPAYTLLVTRREGLVREIVTTRSVID
                     PRGKFDVRVPKGSYELIASADGWATSTPLAVAAGTTDATLKLSAGATLVGVVVDGATG
                     KPLEYARVWREGATGGATASPANAGVVTRADGTFELAGIPPGPFTISVGAGNYNPKLE
                     SGLVARDGATVGPLRVELRKLNPGEEPKMELVGIGVKLSADGEALKVDMVAPEGGAAG
                     AGIVAGDLLVAVDGVPVTQLGIDGAVARIRGVAGTKLSIGVKRGEQVSTLVVERKPVK
                     L"
     gene            11207..11497
                     /locus_tag="VMZ53_26995"
     CDS             11207..11497
                     /locus_tag="VMZ53_26995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32188.1"
                     /translation="MEAALKLTPETYPLTWAEICEQCPNAWVFLVDVEIAEDGSIRWG
                     RLIDHDRSMKQALARVDARDPHVVVAHTKGRPRSSPRIEVTDEIRSIFRASR"
     gene            complement(11494..12147)
                     /locus_tag="VMZ53_27000"
     CDS             complement(11494..12147)
                     /locus_tag="VMZ53_27000"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32189.1"
                     /translation="MRAWMSWSSGKDSAHALAAVRADVVGLVTTVSAAYDRVAMHAVR
                     RALLERQAAALRLPLTVVELPTPCTNDEYEARFGAALVRAREEGVTHIVFGDLFLADV
                     RAYRERQLAAVGLTPVFPLWQRDTRELAHEMIASGLRARLTCVDPRVLERTFAGREFD
                     ESLLADLPASVDPCGERGEFHTFVTHSPLFSTPIAVTLGEVVERDGFVFADCLDAAL"
     gene            complement(12163..12321)
                     /locus_tag="VMZ53_27005"
     CDS             complement(12163..12321)
                     /locus_tag="VMZ53_27005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32190.1"
                     /translation="MSVPFPDSLCHRCTHLRVVTSGKGSQFLMCREPTLPKYPPQPVR
                     VCPGFVAR"
     gene            12408..12899
                     /locus_tag="VMZ53_27010"
     CDS             12408..12899
                     /locus_tag="VMZ53_27010"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HUS32191.1"
                     /translation="MRLFACVAAASVLALGGCSLFMRSIEKPKADVRDVSLSSASFNG
                     VTGELALDVMNPNSFSVPLSGIDWTLSVGGTRAVSGTVELSQTIPAKGVAPVTTSLSI
                     ATTDALAVGAVLARGSRDYQIQVRLHFSTQVGQLDVDVTHAGQLGTQTASTSGPGWTQ
                     LGL"
     gene            12969..13490
                     /locus_tag="VMZ53_27015"
     CDS             12969..13490
                     /locus_tag="VMZ53_27015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32192.1"
                     /translation="MIRASLFALAFASLTMFAGCGKKKDEGGGAAKTAEKSGGGDTKA
                     APAAGPTKLPKLGLQIDAPGKVEVGDAIMGEGHMLQGEAFGAMQIEVAEKKKTLDDAK
                     SDAEMYGPKDLKEETLPDGWAVTYSNTGSMGKNFFVDVQRDIDGKTYHCSTTGSEEKQ
                     QQAVLAACKSLKK"
     gene            complement(13491..14396)
                     /locus_tag="VMZ53_27020"
     CDS             complement(13491..14396)
                     /locus_tag="VMZ53_27020"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HUS32193.1"
                     /translation="MRVVPFLLLLGCGVEPATGSDSHDQPGEVGVAGIQPPRTATLAE
                     VVAWIDAETAVRAPDTQLAITIEDLQTGEHVSLRGDTKHVSASSAKAWWVAAALDGVG
                     IAPVAPYAPNIFTYSDNSASGSVIDLIGPNAVNTWMWTVAGMNDSALTQWSYDKTRIA
                     TNSPRAMGGDNYMTSDDAVRFLARVYRKEIIADERGDKLLAWMTLSPNSGLGGWIPSR
                     LPTSVATTTMHKAGWLPPGCCSDDSYYNTSNDIGIVETPLGRGYAIAILARRGSDYAA
                     QQELMELSSCLLYRAFVQDDQLACE"
     gene            14424..14912
                     /locus_tag="VMZ53_27025"
     CDS             14424..14912
                     /locus_tag="VMZ53_27025"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HUS32194.1"
                     /translation="MADSTVIVQLPSEALARLPSAIAALVPANTDHFMAALEMGYLAA
                     SADGLDETERDAIATTLEKVTGIGFDHDAFEAHFSDLDAAVLALGRRERLARTSAEFD
                     NDDTRANVIRFAALVAMADGVLNAPELVVLEEVGSHFKWTPDRIRGLVTDAAARVQGG
                     DR"
     gene            14909..15502
                     /locus_tag="VMZ53_27030"
     CDS             14909..15502
                     /locus_tag="VMZ53_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HUS32195.1"
                     /translation="MSFVNRMSKEINCKIVYYGAGLCGKTANLQYIFAATKPESRGKM
                     ISLETETERTLFFDFLPLELGKIGEYKVRFHLYTVPGQVFYDASRKLILKGADGVIFV
                     VDSQAARVEANIESLENLESNIAAYGTSLATTPHVVQYNKRDMPEVTPIAELREQLNP
                     HKAPDFEAVAKTGSGVFETLKGLAKRVLTELAKKPPA"
     gene            complement(15499..16719)
                     /locus_tag="VMZ53_27035"
     CDS             complement(15499..16719)
                     /locus_tag="VMZ53_27035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32196.1"
                     /translation="MDSRLQRAIGTPRIQQQAGELVRLALVALEDLKQIDESLYERFV
                     ATRNSKLGPEAVAEGLRKLWDDTFRGLEQMLAFARSLSSQQQEAQATKDKADGDGFDF
                     GDFTEAPSSQNLDIGAGDIGDLLDGLGDASQEDEAAKWNGVLEKVGSIEYGLTSQLKE
                     ARVRVDVSLDTGETNQVLGILDDTNSSSSEGVHALVAAVYETFLPDIHTATVVPGYLT
                     TLGRALLVRRGLADLGLKLTPQNNLLQGDDATEHKIALEQIRLTMRQFVGSVVCRAMR
                     AADRWQMVEFERELSELPLKAARLTSEGLVKYIDSLSSVNQREVLVVHDRKTLEEMRE
                     ALSNARQLIDLSPRAAREMMDRAYQAAQRLRGRSPVSDEMIIQLERYAPTSSNPGEAG
                     KFLERLEAVLAASP"
     gene            16818..17573
                     /locus_tag="VMZ53_27040"
     CDS             16818..17573
                     /locus_tag="VMZ53_27040"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HUS32197.1"
                     /translation="MIGDFSAGIGDGFKGAGYLARHPRLWIWVLLPAVIIAILLILLI
                     GALMTWLSPYIDRVAAHVPGHFADSIIKILLGIVLAIASFSVMFSIASLIAGPFNEML
                     SEAIEEQITGQKGGGFKLGEFLHDIAVGALHALRRIFVYLLTMGLLLVIGLVIPVIGT
                     AIAALGGWIATSRFASYDAYDAIFSRRRMAYRAKLQYLNHNLARTMGLGGVIAFMSII
                     PFVNILALSIGAAGATLRMCAPPAAARQVATRS"
     gene            complement(17560..>18222)
                     /locus_tag="VMZ53_27045"
     CDS             complement(17560..>18222)
                     /locus_tag="VMZ53_27045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HUS32198.1"
                     /translation="DEWGMTPMTVNAYYNPALNEIVFPAAILQPPFFDPNADDAINYG
                     GIGAVIGHEISHGFDDQGSQYDEKGALKNWWTKDDSDKFKTATNMLVAQYSAYCPFPA
                     EGGKAAQCVKGELTLGENIADLAGITVAYQAYKLSLNGKPAPVLDGFTGDQRFFLGFA
                     QVWRRSYRDQELANRLVTDPHSPSEFRTSVVRNLDVWYDAFGAKQGEALFLANDKRVK
                     IW"
BASE COUNT         2932 a         6095 c         5925 g         3270 t
ORIGIN      
        1 gcccgcgtcc atcgtgatcc aggcttcgtc gatgcggaag acgcgcgtct tgtccgggac
       61 gacggagatg atgaccgcgg tgtacgtcac gaagctgcgg tgggcggcca ggccgaggaa
      121 ccggcctttg cgcgatgacc acttggcggc gtcggtgacg cgctcgatca cgttgcggag
      181 gcggccggcg tcgaccgggt gtttgtcgag cggctcgccg tagttcggca gtttcgggat
      241 gccgaggtcg gcgagcgagt gcttcttcgc cgggccgatc acttcgagcc acgtgtcgcg
      301 cgggtctttg ccgcgtgcgt gcgcgagctc gtcgatgaac gagccgatgc cgaatgcgtg
      361 gaagatgttg tagacggagc ggtaccagcc gacgcggacg tgcggtgtgg ctttgcacgg
      421 ctcggcgcga acgttcggga tcgcgagggc gacgtcgacg acgccttgtt ggagatcgcc
      481 gaggcccggg gtgtcggcgc ccggcttgtc gctgaacgtc gtggcgatcg gggtgagtgc
      541 cgtgcggtga tgccaggcgg tgacgttgcc ttgcgcatcg agcgccgcgc gcaggtgttg
      601 cgcgttggcc gcgttgtagt agtcgtggcg gatatcgtct tcgcgcgtcc actgcacgcg
      661 gatcgggatg ccgagctgtt tggcgaggtg cgcggcttcg gcgatgaagt cggctttcga
      721 cttgcgaccg aagccaccac cgaggagcgt gacgttgacg gtgacgtttg ctgcgtcgat
      781 gccgaagatc ttcgcgacgt cctcgcgcgc accttgcggg ttctgggacg gtgcgtacac
      841 ctcgacggtg cggccttgca gcgggcgcac gatcgcggcg ggtggttcca tcgtcaggtg
      901 cgcgaggtgc ggcacgacgt agtccgcctc gacggtcttg gcggctttgc tgaacgccgc
      961 gtcgatatcg ccgacgttgc gcacggcctt gccgccttcg cggacggaag ccagcatctc
     1021 gtcccgcgtg gccttcgagc tcgcgctggc gtgggggccg tggtcccacg tgatgtcgag
     1081 cgcagcgcgg cctttcagcg ccgcccatgt cgtgtctgca acgacggcga cgccacccca
     1141 cgcttggaac tcccacggcg gtttcggggc cggcagctcg atcacatgtt tcacgcccgg
     1201 gatcgcaagc gcgcgctgct tgtcgacctt cgtgaccttg ccaccgagga ccggcgggcg
     1261 cgcgatgacc gcgatcagca tgtccgggaa gcggacgtcg gcgccgtacg tcgcagtgcc
     1321 cgtgacgatc gcttggccgt cgagcagcgg caggttcggc tcggacgtgc gcgtcagctc
     1381 gctcgctggg cgaagcacga tgtcttttgg cttcggcacc ggaagcttcg cggcatcggc
     1441 gacgaccgcg gcgaacggca ccttgcgctt cgactttgcg tgtacgacct cgtgattgat
     1501 cgtcgtgcac gtctcgggct tgacgcccca cttcttcgcg gccgcggcgc acaacatcat
     1561 gcgagccgcc gcaccggctt ggcgcaggct ctcgtattgc tggcggatgc tgctcgagcc
     1621 gtcggtgtct tggtcgccgt acttcttgtc gccttcggct tggcggaccg tgatgcgcgc
     1681 cgggtccgcg ccgagctcgt cggcgatcag cacggggatc gtgctgcgca cgccttggcc
     1741 catctcgctg cggtggcaga agatcgtcgt cgagccatcg agatcgatgt gcacgagcgc
     1801 gtgcggatcg aggccgcgtt tgccttgcgg cgcgggcggc gcagtcgggc cgttcttgcc
     1861 gctcggttct tcggcgtgcg cgtggcgaag tccgatcgcg aggccgcctg tcgtgaggcc
     1921 gagcccgacg agaaacgacc gccgggtgat cttcgatggc gcgctcactt cgtctctccg
     1981 ccgtcgctcg tgccagcagc cttcttgatc gcggcgcgga tgcgcgtgta cgtaccgcag
     2041 cggcacaggt tgcccgccat cgattgatcg atttcgtcgt cggtgggctt cggcttcact
     2101 gcgagcagcg ccgcagcgct catgatctga ccggcctggc agtagccaca ttgcggcacg
     2161 cgcagatcga gccacgcctt ctgcagcggg tgcgagccgt cgggcgagag gccctcgatc
     2221 gtcgtcaccg cttttccttc ggcgcgccgg atcggcgtca cgcacgagcg aacggcatgg
     2281 ccgtcgagat gaacggtgca cgagccgcac agcgcttcac cgcagccgta cttcgtaccc
     2341 gtgaggccga tgacatcgcg gagcgcccac agcagtggca tctccggatc gacctgttgg
     2401 agatcgtggt ccttgccatt gatgcgcagc ttcatcgtgt ctctccggtg ggtgcagcga
     2461 tcggctcggg tgggcattcg gcgccggtct cgacccacgc agccgtcagc tcgccgaact
     2521 gttgttgcgt gccaggcgcc ttctcgcgat ccgcgcccgg attccagccc cacccgacga
     2581 gctcgtcgtg gccgttgtgc tcgacgagct gcgcgagcga cttaccgccg ttgcgcttcg
     2641 gatccttgat ctggttgcag atgtacgcgg cggacttgcc gacccacgcc atctcgatcg
     2701 gcgcgacgtg ccacttcggt gcacccggca cacgcgtgag ctcctgattg cgatcttggt
     2761 gacacgtcgt gcattccatc gcgggcacac cgtgatcgtc cggacctcgg acgaccggcg
     2821 gatcgtgcaa cgcgagctgc atgccttgat gcggcgtatc gccgttggga tggcagttga
     2881 tacagcgcgg atgcatgatc acgcgcgcca tctcgccgaa cagcgcacgc gagcgctcgg
     2941 cgcgatcccc gatcgcatcg aagtcacgca cggccaacag ctcaccggcc gcggcttccc
     3001 gcagctgcgg gttcgcgctg ggcgcagcgg cagatttcgg cccaccgcac gccgcgacgg
     3061 ccaagcagag cgcaagtcgc ctcatgcccg gcagcttgtc tggcggtcag gcgccgaaca
     3121 agcggatgac gacccaatca cgcaggtctc gtcatgtgcg ctcgccgcga ccgttctgcg
     3181 atcagagcgt gcggccgagc aacgaatcgt gcgacgagct tagtgaccga cggagaggcc
     3241 gagctcggcc atcgtgtagc cgcacagcgt gccgccgtac gtgatcgccg actgcgtcac
     3301 cgcgaggtgc agatcgaagt cgcacgtcgc cgagtcgctg ccgctcgtcc acgcgagcga
     3361 gcccgtcatc ccggcggtga agcccgcggc gtcggcggtc gacgtccagt ggatgtcacc
     3421 atcgagcgag ccgctgacgt ccttgcagcc gtcgaatgac gcgctgagat cgaacgctgc
     3481 gtgctcgtcg tcgccctcgc cggcatagtt gccggtgacc gcgacggatc caccgagcgt
     3541 gcacgcgccg gtgtagttga ggttcacgtc cgtcgaaccg gtgccgcccg tcttcgcggc
     3601 ggtgaccgcc tgcgtctgcg cgttggacag cgagatgctc gccgtcgacc agacgcggac
     3661 ggattcttcc ttcgtcggct gatcactgca ggcagcggcg aatacgagcg ggagtaggag
     3721 gaggcgcttc atgccccgca tcgtgcccga agctccgcgc gatccagcca tacgggactg
     3781 gcattggctg gcaagtcgga ttcagtgtct gctcagactg tgcgctcagt cgcgcaaaag
     3841 ccgcggactg ccgtcacttg ggccgcgcgt cacgggcttg tggagtatca ccgtccggcc
     3901 ggcctcacgc cccgctgcgt gcgcgctgga catcgtcatg aaacgccggc gacgacggat
     3961 gcgcggatag cgcgcgcgaa agaatgcgtc gaggtgatca tcgccggacc acacgagggc
     4021 ttcgcttgtc accgcttcgc tcgcgccggc cgatgtgctc gcgccttgcg acgtgctcgt
     4081 gccttgcgat gttgtgctcg tgccttgcga tgtgctcgtg cgtgatgcaa cgagcttctc
     4141 gcgaaacccg cgcacgacgc ctgattgata cgcgcgccga tcgcgcccgc tcttcacgcg
     4201 tgcgtcgcgg cgattttctt cccagagcct ctgtgcggtc gcgcgcatga actcgaacac
     4261 gtgcagcgcc atatcgagat tcgtgcgcgt accgatcacc tcgaagacct ccgcctgcga
     4321 ggcggtcgcg gccacgtaca ccggcacgcg aatcacttcg acgaagaagc attcggtgag
     4381 cagcccgacg atgtcgagct cgacggcatt cgcgcgtctt gtcggctcgc cgagctggcg
     4441 cacttcgtag acgttcgcgc cgccgctgcg tgcttgctcg atgttgtggc gcaacatcag
     4501 ctcgtgcgcg cgacgcatcg cgatctcggc ttcgtgctcg ttcgcgctac cggcgagcgc
     4561 catgagcttg cgaatcttgt cgagcgcgcg ctcaccctcg gcatcggcgg cggcgatcgg
     4621 cgcaccggcc gcacgcgcat cgatcccgcg ctcggcgcac acacgctgaa acgtcgggcc
     4681 atgcgcaggc tctgcggatg cgccgagaac ctcgtcgacg aactgatgcg ccatctcgtg
     4741 ctcgaggaca ctgattacct cgagccatgg ccgctccagc acgagcgtcc tcgacagctc
     4801 gagccgccgc gtcgcgcgcg accactggcc gaggcgcttc gtcgtatcgc cgagcacgag
     4861 caccgcgggc ttcagcttgc cggcgaatcg cttcgtattc gtacgttcgt acgcgcggcg
     4921 cagctcgcgc acgagtgccg cttcgagctc gatcgacaac accggttcag gcgtagcacg
     4981 ccgatccgac agcccgcctc gcgcgcgagc gcacggcgcc tcgagttgcc accacttgtc
     5041 tgaagcgtct tcgagcgtgt gtcgccgcgc acggtgggcg aaaaagtctc gctcctcgtc
     5101 gctaactcgg cattctccgt cgaggagatc tgctcccaaa acttgcacct gcggctggcc
     5161 gtcgtaaagg agagccagtc atgtttgccg tcgttcctcg tccacctctt cgcgagtccg
     5221 ccaagcgcga gcctctcgtc atggcgaggt gttcgtgcga gctgtgggaa ggcgttcgtt
     5281 gcgtgctgcc cgcgggtcac acgggatcgc atttccgccc ggcgacgtcc gaaggctgcg
     5341 agctgcgctg gcgttcgagt ctctagagcg gctcgctcga gtctctagag cggctcgccc
     5401 gagtctctag agcggctcgc ccgagagcgc gtccttgatg atatcgagcg ggatcgtcga
     5461 ctcgttcgag ttcgtcggcc gcatcgttcc gctcgacgca cgcagctgcg agaccatcgc
     5521 cacaccgcat ccgccgcaga agcgatcgct cttaccgttg acgaagccgc agtcacagcg
     5581 cggctgcagc gccgacccac aaccgccgca gtagctgcgc catgcattgt tgagtcgcga
     5641 gcaaacgcac cgtacgagct ccgtcatcgt ggcctccacc taaccgattc ggccacctcc
     5701 cgcattcgtt gaggcccttc gcgtccgtcg ctccgtccct cgcggttgcg gaggttgcgg
     5761 aagttgcgtc cgtcccttag tggtcaggcc agttgttacg ctgggacgcg tgtttggcac
     5821 gcaagccaaa catctccgca tgttaggacg cggtcgccag cggcaccctg ccccacctcc
     5881 gcaaccagga gctggccggt gggcgtcagg gcggactgga gggcacgcgt cgtcgcccct
     5941 cggtcgccgc tctcttcgag ggtcgagcca acgacggtga tcacgtcgaa ggactggccg
     6001 tcgagcacgc cgtcgcgaac atcgccgcag atgacgcgcg cgggatgcgg atgaccttcg
     6061 cgccgctggc gcagctcgag aatgcggcag atgtctgcat cgcgatcgat gctcgtgatg
     6121 cgaccgccga tgacgtgacc gagccagtcg tcgaacagcc cgaccccaat gccgacattg
     6181 agcactaaca gcgggcgttg cgttggcaac cgcgcgatca cgaatgcacg cagctcgtag
     6241 tcgagccacg acgcctcgag cgaggtgttc cacggctcgc gtccacgcgc gatgtagtgc
     6301 gcgagaagct cggcgtcctc ggatgtgcgg cgtttaccga gctcgtcgtc ctcggatgcg
     6361 cggcggttac cgagctcgtc gtcgaatgcg gtcacgctcg cagaaccgcg ctgtggctcg
     6421 cgatgttccc gcggctcacg atcttcgcgc ggctcgcgat gttcccgcgg ctcacaatct
     6481 tcgcgcggct cgcgatgttc ccgcgatctt cgtgcggctc gcgatgttcc cgcggctcag
     6541 aacggaatgt cgtcggtctc gacgcgaccg aactgcgact cgtcgatgcg gggtggcatc
     6601 tcggaatcac gcggatcgcc ggagcctcgg ccgtggccgt tcccgttgcc gttgccgcgc
     6661 gccgagcgac ctgcacgggc aggctcgatc ttccacacgt cgaggctgtt gaagtacttc
     6721 acttcgcccg acgggctgcg ccactcgcgg ccgcgcagcg agaactcgat ccggacttcg
     6781 tctccgacgc gcagatcgtc cagttgagcg cagcgatcgc ccgtgagctg gaacagcacg
     6841 gtctgcggat acttcggatt gtcggcaagc tcgatcacga actcgcgttt gctgaagcgc
     6901 tcggacacct gcttcgtatc gaagatggtg tggagcttac cggtggtctc gattcccatg
     6961 acgagcagca tacgaggtgg gtctgtcaga gtaggctcga cgagcccgtg caatccctcc
     7021 gcgacattgc ccaggctgcc cggcgcgagc tgatgttgtg cggagttgcg ctcgaagacg
     7081 cccatacgtc actgcccagc aagctgctcg cactcccgcc ggtcgtgacc gcgccgatgc
     7141 gtgcgtggcg gctgtttcgc gcccgccggc accgggccgc cgcgaagaag catctcgacg
     7201 agctgcacat cgcgctgcac gagcgcgcgt cactcggcgt gctcaacgca gacttgtgga
     7261 gcgcgatcat gcgcggcgtc gatgcgcttg cactctcgcg gtggctcgcg accgacgagc
     7321 tcacggccgc ggatgttcgg ccctcgcggc agaccgtgca gatcattctt cgtgatctgt
     7381 cggagctaat cgagctcttg agtgagctcg aagggcctgg ggaacgggct tccggctcgt
     7441 cgccgccgtc gtccacgtga ccatcgcggc gagctgcatc cacgccgcag cccacgtcac
     7501 gtatgcgccg tgataccagc tcgagaacag accgacgacc ggaaagaacg cgatgaatgc
     7561 gatcgcgatc agcgcaccgc acaatccggc gaggtcttca tactcgccca tcacgcgagg
     7621 aaaccatcgc cacagcactg gcagcacgcc gatgagcgcg agcgcgatca tcatcggtgc
     7681 ggtgctcgta tcgaatgcat ctctatcgtg gccgttgcag tcgtggaact gcggcatgaa
     7741 taggcaaacg atgagcagcg tgctggacgg taacgacgtg aaccatgtga accaatctgt
     7801 ctttagtgga cgggccatga atgccgcatc gagcagtgaa tgtgccgctc gggtgcaacg
     7861 tctgcgtggc agctcacacg cagtggtcgc tgcgagatgc acgctcgtta cgcagcgctg
     7921 cgttggcgtg gtggcgtcgc gtgctcgcca cagccgttga ccgcgatgtc atagtaggcg
     7981 cgtggatccc gtgaccttgg ccgttctcgg cgtaggagcc gcgggagccg caggcgtgtg
     8041 gctcaagcgc tggttccgac cagaacagcg cacgcgccgg ctgctccgca gaacgcgggt
     8101 gacgccgatc aaggagctga cggatggcca gctcgcatgc gtcgtcggca cgatcgtacg
     8161 agaaggcgag ctgatcgaat cgctgatcga gagaaagcgg tgcgttgcat tcgataccga
     8221 gacaaacgtg ttcgacggca caggcttctc gtcacccgtg cggttcgaga cgacgcgtcg
     8281 catggtgccg ttctatgtgg tggatgccac aggtcgcgtg cgggtcgacg caccgcaggt
     8341 cgcgctgtcg aacaagccga tcgcgaaggg ccagagcttc gtcgagcgcg tcctggtcga
     8401 aggccagcgc gtgcggctcg taggcagcgt cgtcctcgat ccgacgatgg cgggcaacgc
     8461 cgagcatcac ttccgcgaga cgggtggcgt gcacgcgacg ctcaccggta ccgcgaagtt
     8521 ccctctcctc gcggatgagg actagagctt caccggcttg cgctcgacaa cgagcgtcga
     8581 cacctgctcg ccgcgcttca caccgatcga cagcttcgtc cccgcgacgc cgcgaatacg
     8641 cgcgaccgca ccgtcgatgc cgagctgcgt gacaggcacg ccgtcaaccg cgacgagcaa
     8701 atccccggcg acaatgcccg cacccgcggc tccgccctcc ggcgcgacca tgtcgacctt
     8761 cagcgcttca ccgtcggccg aaagcttcac gccgatgccg acgagctcca tcttcggctc
     8821 ctctccggga ttgagcttgc gcaactcgac gcgcagcgga ccgaccgtcg ctccatctcg
     8881 cgcgacgagc ccgctctcga gcttcggatt gtagttgccg gcgccgacag agatcgtgaa
     8941 cgggcccggc ggaatcccgg cgagctcgaa cgtgccgtcc gcacgcgtca cgacaccggc
     9001 attcgccggc gaggcggtcg ctccgccggt cgcaccctcg cgccacacgc gtgcgtactc
     9061 gagcggctta ccagtcgctc catcgacgac gacaccgacg agcgtagcgc ccgcgctgag
     9121 cttcagcgtc gcatcggtcg tgccagcagc gaccgcgagc ggcgtgctcg tcgcccagcc
     9181 atccgcggac gcgatcagct cgtacgatcc cttcggtacg cggacgtcga acttgccacg
     9241 cggatcgatc acggagcggg tcgtcacgat ctcgcggacc aaaccctcgc gccgcgtgac
     9301 gagcaacgtg tacgccggca cgggctgatc gtccgcgttc cggacagtac ccgcgagcgg
     9361 caaccccgca tcgagcgtga tcgtgacgtt ctgcgtgccg cccgcgatct tgtcgagccg
     9421 tgcttgcgcg tgatcttcat cctcgccgat cagcgtgtac gtcttgcgat ccaatccctc
     9481 gagcacgaac gcgccatcgt taccgctcgt cgcaaacatg accgaccgtg gcgcatcacg
     9541 ctgcgccgtc gcaggcgaat cttcgggcac cgcggtgacg agcacgtccg gcacgggcgc
     9601 cccacttccg tcgacgacct tgccgcgaat cgtcgcatcg cgcgccggcg cattgccgat
     9661 cttgatcgtc agcttgtgcg tcgtctgcgc attgccgtcg agcaccgccc acccgcggag
     9721 gttgcccttc gtcgcctcga agaccgtttc atcctgcgca tgaaagacga actgcccctg
     9781 cttgtcggtt gtccactcgt tgacgacctt ctcgaggctc tgctcgccgc tcggcggatc
     9841 cgcgagcttc acccgcgcac ccgccacagg cgcgccactt ccgtcgacga cgagcccgta
     9901 gtaatcgagc gcgggctgca gaaacaacgt cagcccacga accgcctgct tgctcgcgag
     9961 cgcgacgcgc accggtgaat gctcgagctc cggcgcatac ggcagaaagc ccggcgccag
    10021 aatcgtcgcg agcgcgaacg tgcccggctt cggcggagca agctcgaagg cgccgccctt
    10081 gccgctcttg atcgtcgtcg cgccggctgt cgacgtgaat gtgatctcgg cgtccatcac
    10141 gccctcgccc gtcgaccaat tcacgacgcg tccgctgatc acaccgccct ccgccgccac
    10201 ggtcgacacg acgacgcggc cctgccccgg cacaggttcg ggtgccgcgc tgcccgtctc
    10261 gccactgcct gcagcctcga cgatgatctt gccgctgccc gccgccgagc ccgtgcccgg
    10321 cgccttgctc gtcgcaatcg aggggctgct tgtcgaaggt ccatcgccac gaagcttgta
    10381 gatcgcaaat ccaccgccac cgagcagcaa cagcactgcg atcaccgaaa cgattgtcgt
    10441 cttcgctttc acgagaacag ctcctttgag aagcgatgag gtagtggtcg cagtagatgt
    10501 gctcgacgcc ttcgatgcga tggccggcgc gcccttgaac gcggtgaacg gcgcgagcgc
    10561 gcgctgccaa cgcggcgatt tctcgtcgag cgatgcgcgc agccgctcga gccccgtctt
    10621 gaggcgccag cgaacggtgc ctgcgggcac gccgagtgcg cgtgcgatgt ccgcggccga
    10681 gtcgccgtcg aggtagcgcc ggacaatggt cgtgcggaat ggctcttcga gcgcgacgag
    10741 agcaccggcc agcttctcga gcacgcgtgc acgatcgatc gcgtcgacgg cgtcgactgc
    10801 ttcgtgctgc gtggtcgcgg ttcccgctac gtcgcttgca tcgccgcgcg tcgcggcaag
    10861 ctcgagctcc gacgtctgct cgcgtgcgat gcgccttgcc cgcgcgcgcc gatccatccg
    10921 ccagcggttg cgcaggacct tcgcgagcca cgggcgcggt gatccgtcgg cttcgactgt
    10981 gggcggatgt gtgatcgcgg cgatcgccgt ctcttgcagc agatcctcgg cgtcggcatc
    11041 gccgtggacg agcgactgcg ccagcgcacg caggccagcg agctcgcgca agacgacatc
    11101 cggggtcaca gccatgacat gccgcaggtg ggcagagtgt tggcgacgag atcccgggtc
    11161 acgatcgaaa agttgccaca cataccgaag cgaagacgat ggaaggatgg aagccgccct
    11221 caaactgacg cccgagacgt atccacttac gtgggccgag atctgcgagc agtgtccaaa
    11281 cgcgtgggta tttctcgtgg acgtcgagat cgcggaagac ggctcgattc ggtggggccg
    11341 cttgatcgat cacgatcgat cgatgaagca ggcgctcgca cgcgtcgatg cgcgtgaccc
    11401 gcacgtggtc gtcgcgcata cgaagggccg gccgcgcagc tcgcctcgca tcgaggtgac
    11461 cgacgagatc cgcagcatct tccgcgcttc gcgttagagc gcagcgtcga gacaatccgc
    11521 gaacacgaag ccgtcgcgct cgacgacctc gccgagggtg accgcgatcg gcgtggagaa
    11581 cagcgggctg tgcgtgacga acgtgtgaaa ctcgccgcgc tcgccgcacg gatcgacgga
    11641 cgccggcaga tccgcgagca gcgactcgtc gaattcgcga ccggcgaacg tacgttcgag
    11701 cactcgcgga tcgacacacg tcaggcgcgc gcgcagcccc gatgcgatca tctcgtgtgc
    11761 gagctcgcgc gtgtcgcgct gccacagcgg gaataccggc gtgagcccaa ctgccgcgag
    11821 ctgtcgctct cgatacgcgc ggacgtccgc gagaaataga tcgccgaaca cgatatgcgt
    11881 gacgccctct tcgcgcgcgc gcacgagcgc tgccccgaac cgcgcttcgt actcgtcatt
    11941 cgtgcacggc gttggcagct cgacgacggt cagcggcaga cgaagcgcgg cggcctgacg
    12001 ctcgagcaac gcgcgccgca ctgcgtgcat cgcaacgcga tcgtatgcag cgctcaccgt
    12061 cgtgacgaga ccgacgacgt cagcccgaac agccgcgagt gcgtgcgcgc tgtccttgcc
    12121 gctgctccac gacatccatg cgcgcatccg tgcctcgtct tatcaccgcg cgacgaagcc
    12181 tgggcacacg cgaacgggtt gcggcggata ctttggcagc gtcggctcgc ggcacatcag
    12241 gaactgcgag cccttgccgc tcgtaacgac gcgcaagtgc gtgcagcggt ggcagagcga
    12301 gtcgggaaac ggcacggaca cggtttcaca gctgttttcg cgcgttcaca cccggcgagc
    12361 aagatcgagc acttggcgac ggcctgctgc ttgctcctac cccgggcatg cgtttgttcg
    12421 cctgtgttgc tgctgcgtct gtgttggccc tcggtggttg ctcgctgttc atgcgctcga
    12481 tcgagaaacc aaaggcggat gtccgcgatg tgtctctatc gtcggcgagc ttcaatggag
    12541 tcaccggcga gctcgcgctc gacgtgatga acccgaatag cttcagcgtt ccgctgtctg
    12601 gcatcgactg gacgctcagc gtcggtggca cgcgcgcggt cagcggcacc gtcgagctgt
    12661 cgcagacgat tcccgcgaaa ggcgttgcgc ccgtgacgac ctcgctgtcg atcgccacga
    12721 ccgatgcgct ggccgtcgga gcggtgctcg cgcgtggctc gcgtgactat cagatccaag
    12781 tccgcctgca tttctcgacg caagtcggcc agctcgacgt cgacgtgacc cacgccgggc
    12841 agctcggaac ccagaccgcg tcgacgagtg ggcccggctg gacccagctc ggtttgtaac
    12901 ctcgcggatg tacgctcccg acattcggca cgccgcttgg actaggctgc ggaccggagg
    12961 tcacgttcat gattcgcgca tccctgtttg ctctcgcatt tgcttcgctc acgatgttcg
    13021 ccggctgcgg caagaagaag gacgaaggcg gcggcgccgc caagacggct gagaagtcgg
    13081 gcggtggtga caccaaggcg gctccggccg caggtccgac gaagctgccc aagctcggac
    13141 tccagatcga tgcccccggc aaggtcgaag tcggcgatgc catcatgggc gaaggccaca
    13201 tgctgcaagg cgaggcgttc ggcgcgatgc agatcgaagt cgcggagaag aagaagaccc
    13261 tcgacgacgc gaagagcgac gccgaaatgt acggcccgaa ggatctgaag gaagagacgc
    13321 tgcccgatgg ttgggccgtc acctacagca acacgggctc gatgggtaag aacttcttcg
    13381 tcgatgttca gcgcgacatc gatggcaaga cctatcactg ctcgacgacc ggcagcgaag
    13441 agaagcagca gcaggctgtt ctcgccgcgt gcaagtcgct caagaagtaa ctactcgcaa
    13501 gcgagctgat cgtcctgcac gaacgctcga tagagcaggc aggacgacag ctccatcagc
    13561 tcttgctggg cggcgtagtc gctgccgcgg cgggcgagga tcgcaatcgc gtatccgcga
    13621 ccgagcggtg tctcgacgat gccgatgtcg ttcgacgtgt tgtagtagct gtcatcgctg
    13681 caacaaccgg gcggaagcca gccggccttg tgcatcgtcg tcgtggcaac gctcgtgggc
    13741 aggcgcgacg ggatccaacc gccgaggccc gagttcggcg acagcgtcat ccacgcgagg
    13801 agcttgtcgc cccgttcgtc ggcgatgatc tctttgcgat acacgcgggc gaggaaacgc
    13861 acggcgtcgt cggacgtcat gtagttgtcg ccacccatcg cacgtggtga gttggtcgcg
    13921 atgcgcgtct tgtcgtagct ccactgcgtg agcgcgctgt cgttcattcc cgcgacggtc
    13981 cacatccacg tgttgacggc gttcggtccg atgaggtcga tcacgctgcc cgacgcggag
    14041 ttgtcggagt acgtgaagat gttcggcgcg tacggggcga ccggtgcgat gccgacgcca
    14101 tcgagtgccg cagccaccca ccacgctttc gccgagctcg cgctgacgtg cttggtgtcg
    14161 ccgcgcagcg agacgtgctc gcccgtttgc agatcttcga tcgtgatcgc gagctgggta
    14221 tccggcgcgc gcaccgcggt ctctgcgtcg atccacgcga ccacttcggc gagggtcgcc
    14281 gtcctcggcg gctggatgcc cgctacgcca acctcgcccg gctggtcgtg tgagtcgctg
    14341 ccggtggcag gctcgacgcc acagccgaga aggaggagga acgggacaac gcgcacgcga
    14401 aaggtaggtt accatcgctc tctatggctg actccactgt catcgtgcag ttgccaagcg
    14461 aggcactggc acgattgcca tctgctatcg ccgctctcgt accggcgaac accgatcact
    14521 tcatggctgc gctcgagatg ggttatctcg cggcatcggc cgacggtctc gatgaaaccg
    14581 agcgcgacgc gatcgccacc acgctcgaga aggtcaccgg tatcggcttt gaccacgacg
    14641 cattcgaggc gcacttctcg gatctcgacg ccgcggtctt ggccctcggc cgtcgcgaac
    14701 gtctcgcgcg cacgtccgcg gagttcgaca acgacgatac acgcgcgaac gtcatccgct
    14761 tcgccgcgct cgtcgcgatg gccgatggcg tgctcaatgc gcccgagctc gtcgtgctcg
    14821 aagaagtcgg ctcgcacttc aagtggacgc cggatcggat ccgggggctc gtcaccgacg
    14881 ctgctgcgcg tgtgcaggga ggtgaccgat gagcttcgtc aaccggatgt ccaaggagat
    14941 caactgcaag atcgtctact acggtgcagg tctctgcgga aagacggcga acctccagta
    15001 catcttcgcg gccacgaagc ccgagtcgcg cggcaagatg atctcgctcg agaccgagac
    15061 cgagcgcacg ttgttcttcg acttcctccc gctcgagctc ggcaagatcg gcgagtacaa
    15121 ggtccgcttc cacctctata cggtgcccgg ccaggtcttc tacgacgcga gccgcaagct
    15181 gatcctcaag ggcgccgacg gcgtgatctt cgtcgtggat tcacaggctg cgcgcgtcga
    15241 ggcgaacatc gagtcactcg agaacctcga gtcgaacatc gccgcctacg gcacctcgct
    15301 cgcgaccacg ccgcacgtcg tccagtacaa caagcgcgac atgcccgagg tgacgccgat
    15361 cgccgagctg cgcgagcagc tgaaccctca caaggcgccg gacttcgagg ctgtcgcgaa
    15421 gaccggttcg ggcgtgttcg aaacgctcaa ggggctcgcg aagcgcgtgc tcaccgagct
    15481 cgcgaagaag ccgccggcct aaggcgaagc cgcgagcacg gcctcgagcc gctcgaggaa
    15541 ctttccggcc tcgccggggt tgctcgatgt cggtgcgtaa cgctcgagct gaatgatcat
    15601 ctcgtcgctg acgggtgagc gcccgcgcag ccgctgcgct gcttgatacg cgcggtccat
    15661 catctcgcgt gcggcgcgcg gcgagagatc gatcagctgg cgcgcattcg acagcgcctc
    15721 gcgcatctct tcgagcgtct tgcgatcgtg aacgacgagc acttcgcgct ggttcaccga
    15781 cgacagcgag tcgatgtact tcacgaggcc ttcgctcgtg agacgcgccg ctttgagcgg
    15841 tagctccgag agctcgcgct cgaactcgac catctgccag cggtcggcgg cgcgcatcgc
    15901 gcgacacacg acggagccaa cgaactgccg catcgtcagg cgaatttgct cgagcgcgat
    15961 cttgtgctcc gtcgcgtcgt cgccctgcag caagttgttc tgcggcgtga gcttgagacc
    16021 gagatcggcg agcccgcgac gcacgagcag cgcgcggccc agcgtggtca ggtagccagg
    16081 caccacggtc gcggtgtgga tgtcgggcaa gaacgtctcg tacactgcag cgacgagcgc
    16141 gtgcacgcct tcgctcgacg agctgttcgt atcgtcgagg atgccgagca cctgattcgt
    16201 ctcgcctgta tcgagcgaaa catcgacgcg cacgcgcgct tctttgagct gcgacgtcag
    16261 gccgtactcg atcgagccaa ccttctcgag cacgccgttc cacttcgcag cctcgtcttc
    16321 ttggctcgca tcgcccaggc catcgagcag atcgccgatg tcacctgcgc cgatgtcgag
    16381 gttctggctc gacggagctt cggtgaagtc gccgaagtcg aagccgtcgc cgtccgcctt
    16441 gtccttggtc gcctgcgcct cttgctgctg cgacgacagc gaccgcgcga acgcgagcat
    16501 ctgctcgagc ccgcggaacg tgtcgtccca gagcttgcgc agaccctcgg ccacggcttc
    16561 gggcccgagc ttgctgttac gagtcgcgac aaagcgctcg tagaggcttt cgtcgatctg
    16621 tttcagatcc tcgagtgcga caagcgcgag ccttaccagc tcgccggctt gctgctggat
    16681 tctcggcgtg ccgatggctc gttgaagtcg tgagtccacc gctggtcagc agtttcgcac
    16741 gctctgcaag tcccggccaa ttccggcaat cagctcgtcg gtggcataag ctcagcagat
    16801 tcgggggtag cccaggcatg atcggcgact tttcggcggg aatcggcgat ggattcaagg
    16861 gtgcggggta cctcgcgcgt cacccgcgct tatggatctg ggtcctcctg cccgccgtca
    16921 tcatcgcgat tctgttgatc ctgctgatcg gcgccctcat gacctggctc tccccctata
    16981 tcgacagggt ggccgcccac gtcccgggtc acttcgccga cagcatcatc aagatcctac
    17041 tgggcatcgt cctcgccatc gcgagcttct cggtgatgtt ctccatcgcc tcgctgatcg
    17101 ccggcccctt caacgagatg ctctccgagg cgatcgagga gcagatcacc ggccaaaagg
    17161 gcggcggctt caagctcggg gagttcctcc acgacattgc cgtcggcgct ctccacgccc
    17221 tccgccgcat cttcgtgtac ctcctgacga tgggccttct gctcgtcatc ggcctcgtca
    17281 ttccggtcat cggcacggcc atcgccgcgc tcggcggctg gatcgcgacg tcacgcttcg
    17341 cgagctacga cgcctacgac gcgatcttct cgcgccggcg catggcctac cgcgcaaagc
    17401 tgcagtactt gaaccacaac ctcgcgcgga cgatggggct cggcggggtg atcgccttca
    17461 tgtcgatcat ccccttcgtc aacatcctcg ccctctcgat cggagcggca ggggcgacac
    17521 tgcgcatgtg cgcgccgccc gctgctgctc gtcaggtcgc taccagatct tgacgcgctt
    17581 gtcgttcgcg aggaacagcg cctcgccctg cttcgcgccg aacgcgtcgt accagacgtc
    17641 gaggttgcgc acgacggacg tgcggaactc cgacgggctg tgcggatccg tgacgagacg
    17701 gttcgccagc tcttggtcgc ggtagctgcg gcgccacacc tgggcaaagc cgaggaagaa
    17761 gcgctggtcg cccgtgaagc cgtcgagcac cggtgcaggc ttgccgttca gcgacagctt
    17821 gtacgcctgg tacgcgaccg tgatgccggc gaggtcagcg atgttctcgc cgagcgtcag
    17881 ctcacccttc acgcattgcg cggccttgcc gccttcagcc gggaacgggc agtacgcgct
    17941 gtactgcgcg acgagcatgt tcgtcgccgt cttgaacttg tcggagtcgt ccttcgtcca
    18001 ccagttcttg agcgcgccct tctcgtcgta ctggctgccc tggtcatcga agccgtggct
    18061 gatctcgtga ccgatgaccg cgccgatacc accgtagttg atggcgtcgt cggcgttcgg
    18121 atcgaagaac ggcggctgga ggatggcggc cgggaacacg atctcgttga gcgccgggtt
    18181 gtagtaggcg ttcaccgtca tcggcgtcat gccccactcg tc
//
LOCUS       DATLBZ010000540         5495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1451402, whole genome shotgun sequence.
ACCESSION   DATLBZ010000540 DATLBZ010000000
VERSION     DATLBZ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451402"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VMZ53_27050"
     CDS             <1..288
                     /locus_tag="VMZ53_27050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32199.1"
                     /translation="FDANGVELWSHRYAALALNNPVLSTTGDILLEAAHEGLHGSRYD
                     VLVLDGATGAERATIRSCGYINVLAADAQAYYGLGLTGSDSVGLARFSMTP"
     gene            complement(285..923)
                     /locus_tag="VMZ53_27055"
     CDS             complement(285..923)
                     /locus_tag="VMZ53_27055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32200.1"
                     /translation="MLRATARFARLALHAAVLLALAGCRDTTKPARPDSAPYDTTTAD
                     ASKAAACSSVFGSALTDSFGRADGTILAVVPPGHPTCPQPNGTHLVLQVTMNGAVYRM
                     VVNIDVLTHEVDAPLAGDPWSEGWHPTAQLDYVATLGVHANDFDLNPDVDGWVTNQLE
                     LGAHVSVFATSENFHDSAHLVHRENTNTDGAIVVNPETSPHYLLFRFSNQAF"
     gene            complement(927..2870)
                     /locus_tag="VMZ53_27060"
     CDS             complement(927..2870)
                     /locus_tag="VMZ53_27060"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS32201.1"
                     /translation="MIVLEILQGTPGGEQRVYRFSGSSASISIGRSPASSIPVTDFHL
                     SGDHAQITQIGEHYVFRDLRSTNGSAVEREGRRIAIDASARWELALVDGDFLLLGNRN
                     DPVRIAVRIGDESEEELGDRLVASRSIMDLPKVTDQIEGREPAEALRIYKALQPLGAR
                     LETNGVIEAIVDATFQLLPRATHVAILLRSESDKDRFALAVSRQRPVAGKEGPSIDPV
                     RASRAVLRRVLADRAAILTANAQEELSSSESIMGGQILSMLAIPLWRGDDIIGLIQAD
                     NRHSAGMFHERELETALLLGAQAALAIDNATLVARLRIAEERARGENVYLKRREQKIK
                     FDNIIGDSPAMKALLAQLERVIDTRATVCIEGETGTGKELIASAIHYQSSRRDKMFVA
                     QNCAALPENLLESELFGHKRGAFTSADSDKKGLFEIADGGTLFLDEMGEMPMSLQAKL
                     LRVLQEGTIRPVGATSEKQVDVRILCATNRDLAAEVEKGRFRQDLYYRLMVFPIKLPP
                     LRDRREDIPELGAHFLKRYAEEYRVELPGFTQDALDALASYNWPGNIRELENEVQRLV
                     IQAESGHWIEVTDLSPRLRKIEGTVTRIAPKQGTLKEMMEQVERWLIAEALRDHGNNK
                     TKTAATLGITREGLHKKLAKFGV"
     gene            3038..>5495
                     /locus_tag="VMZ53_27065"
     CDS             3038..>5495
                     /locus_tag="VMZ53_27065"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUS32202.1"
                     /translation="MLPSTGDLALDADAGVVVVGGTDGNAVELVQRAKKALGAKSLDV
                     PVISTARGVDRAKLEAAGADEVLTRPAYLRDVITIGRILHGIPASKRNHIVGSLAETT
                     GVYTLVRALASLGRSAVLTLIRGLRRGEVRFYKGEVTSAEVGLIHGQAALHQLLLWTD
                     ARWDFAHEDIVRRQQIPLSPDELFADAERFLEGVRESAGHLSPATVLEQDVQRIQDLG
                     KQIPTEVHGVLRMFDGHRVLTDVLEDSPYRVFETLRVAQKAVEAGLLRKVDSQRPKAT
                     WRAVLAIDEWLVGSETRAAVVERTADLTDSEPVGKKKAGKGKARAKRRKAATPVAGVK
                     AEIDWGALVPRVIGAEVGPLSGVVPAQATAGEVELPTRDRAREKLEALMDTDKRQKIF
                     PTEIGLEPKVVIDSGMGGDTDEWERIELEAKKKLAVAEASNEPETLGSPKRKKRKQDR
                     LAAAAADKASAEKAPAEKADAENTAAEHAAPRQATTQQATTQQAATQQATTQQATTQH
                     ATTQQATPQQATPQQATTQQATTQQAASQTIAADRGPAVDEVAAAAKLVAEKAAADAK
                     AAAERASVHANAADNAREAAARAAAEASAATTAVAAAEHAATVGKAAADARAASDRAA
                     TDAKAAAEFAAAASRAAVDSMAAAHAKAAVERSAADAAAAAEASEAAARLAADAKAAV
                     DARLAADAKVIAEAKATAEANLVGARTEADRASMAASAADNARAAAERAAADAAAPMT
                     DATSSNDYAVAAAAAASDARTAANRVASHAKTAAEFAAAAARAAGDSMAGAHAKAAAE
                     RAATDAAAAADASRAAERAAA"
BASE COUNT          949 a         1886 c         1843 g          817 t
ORIGIN      
        1 ttcgacgcga acggtgtcga gctgtggtcg catcgctacg ccgcgctcgc cttgaacaat
       61 ccagttctat cgacgaccgg cgacatcctg ctcgaggcgg ctcacgaagg tctccatggg
      121 tctcgctatg acgtgctcgt cctcgacggt gcaacgggcg cagagcgcgc gacgatccgg
      181 tcgtgcgggt atatcaacgt gctcgccgcg gacgcgcagg cctactatgg cctcggcctg
      241 accggctcgg attccgtcgg actcgcacgg ttctcgatga cgccttagaa ggcttggttc
      301 gagaagcgaa acagcaggta gtgcggcgac gtctcgggat tcaccacgat cgcgccgtcg
      361 gtgttcgtgt tctcgcgatg aacgagatgc gcgctgtcgt ggaagttttc gctcgtcgca
      421 aacaccgaga cgtgcgcgcc gagctcgagc tgattcgtaa cccagccgtc gacgtcgggg
      481 ttcagatcga agtcgttggc gtggacgccg agcgtcgcga cgtagtcgag ctgcgcggtc
      541 ggatgccagc cctcgctcca tggatcgccc gcaagcggcg cgtcgacctc gtgcgtgagc
      601 acgtcgatgt taacgaccat gcggtagacc gcgccgttca tcgtgacctg caggacgagg
      661 tgcgtgccat tgggctgcgg gcatgtcggg tgcccgggcg gcacgacagc gaggatcgtt
      721 ccgtcggcgc ggccgaagct atcggtcagc gcgctgccga agaccgacga gcaagcagcg
      781 gccttcgagg cgtcggccgt cgtcgtgtcg tacggcgcgg agtctggacg tgcgggcttc
      841 gtcgtgtcgc ggcatccggc gagcgcgagc aataccgccg cgtgcagcgc caggcgagcg
      901 aaacgagctg tcgcgcgaag cacgacctag accccaaact tcgcgagctt cttgtgcagg
      961 ccttcgcgcg tgatgccgag cgtggcggcg gtcttcgtct tgttattgcc gtggtcgcgg
     1021 agcgcttctg cgatcagcca gcgctcgacc tgctccatca tctctttcag cgtgccttgc
     1081 ttcggcgcga tgcgcgtgac ggtgccctcg atcttgcgca gccgcggcga gaggtcggtc
     1141 acttcgatcc agtgaccaga ctccgcttgg atcacgaggc gctgcacctc gttctcgagc
     1201 tcgcggatgt tgcccggcca gttgtaggac gcgagtgcgt cgagcgcgtc ttgcgtgaag
     1261 cccggcagct cgacgcgata ctcctcggcg taccgcttga ggaagtgcgc accgagctct
     1321 ggaatgtctt cgcggcgatc gcgaagcggc ggcagcttga tggggaacac catcaggcgg
     1381 tagtagaggt cctggcggaa gcggcccttc tcgacttcgg ccgcgagatc gcgattcgtc
     1441 gcgcacagga tgcgaacgtc gacctgcttc tcgctcgtcg caccgaccgg gcgaatcgtg
     1501 ccttcttgta ggacgcggag cagcttcgct tgcagggaca tgggcatctc gcccatctcg
     1561 tcgaggaaca gcgtgccgcc atcggcgatc tcgaagaggc ccttcttgtc gctgtcggcg
     1621 gaggtgaacg cgccgcgctt gtgaccgaag agctcgctct cgaggaggtt ctccgggagc
     1681 gccgcgcagt tctgcgcgac gaacatcttg tcgcgacgcg atgactggta gtggatcgcg
     1741 ctcgcgatca gctccttgcc cgtgcccgtc tcgccttcga tgcaaaccgt cgcgcgcgta
     1801 tcgatcacgc gctcgagctg cgcgaggagg gccttcatcg ccggcgagtc gccgatgatg
     1861 ttgtcgaact tgatcttctg ctcgcgtcgc ttgaggtaga cgttctcgcc gcgtgctcgt
     1921 tcttccgcga tgcggagccg cgcgacgagt gtggcgttgt cgatggcgag tgcggcctgc
     1981 gcaccgagga gcagagcggt ttcgagctcg cgctcgtgga acatgccggc ggagtggcgg
     2041 ttgtcggcct ggatgaggcc gatgatgtcg tcaccgcgcc agagcgggat ggcgagcatc
     2101 gagagaatct ggccgcccat gatcgactcg ctcgacgaca gctcttcttg cgcattcgcg
     2161 gtgaggatcg ctgcacgatc cgcgagcacg cggcgcagga cggcacggct cgcgcgcacg
     2221 ggatcgatcg atgggccttc tttgccggcc accggacgct gccgtgacac ggcgagcgcg
     2281 aagcgatctt tgtcggattc cgagcgcagg aggatcgcga cgtgcgtcgc acggggtagc
     2341 agctgaaatg tcgcatcgac gatcgcttcg atcacgccgt tcgtctcgag acgcgcgccg
     2401 agcggttgca gcgccttgta gatgcgcagc gcttctgccg gctcccgacc ttcgatctgg
     2461 tcggtaacct tcggcaggtc catgatcgag cgcgacgcca cgagccggtc accgagctcc
     2521 tcttcgctct cgtcaccgat gcgaaccgcg atgcggaccg gatcgttgcg attgccgagc
     2581 agcaggaaat cgccatcgac gagcgcgagc tcccaccggg cactcgcatc gatcgcgatg
     2641 cggcggcctt cgcgctccac ggcactcccg ttcgtcgatc gaagatcgcg gaatacgtag
     2701 tgctcgccaa tctgcgtgat ctgcgcgtgg tcgccggata agtggaaatc cgtgacggga
     2761 atgctgctcg caggcgagcg accaatggag atggacgcag atgacccgct aaagcgataa
     2821 acgcgttgct cgccgcccgg cgtgccttgc aggatctcga gaacgatcac gtggtaaacg
     2881 aagagcgccc gtcggcgact ccctccgtgg gtttatactg caccctcgat cttcgcgcat
     2941 gtcttcggga ccttccaaag tcatcgtcct ggacccggac cctcgggccg gtcgacaggt
     3001 ccagctcggt ttcgagcggg aaggccttgc cgcggttgtg ctccccagca caggggacct
     3061 cgcgctcgac gcggacgcag gcgtcgtcgt cgtcggcggg accgatggca acgcggtcga
     3121 gctggtccag cgcgccaaaa aggcgctcgg cgcgaagagt ctcgacgtcc cggtcatctc
     3181 gacggcccgc ggcgtcgacc gcgccaagct cgaggcggca ggcgccgacg aggtgctcac
     3241 gcgtcccgcg tacctgcgcg atgtcatcac aattggccgc atcctccacg gcatcccagc
     3301 gagcaagcgc aaccacatcg tcggaagcct cgccgagacg acgggcgtct acacgctcgt
     3361 tcgcgcgctc gcctcgctcg gccgctccgc ggtcctgacg ctcatccgcg gcctgcgtcg
     3421 cggcgaagtc cgcttctaca aaggcgaggt cacgtcggcc gaggtcggcc tgatccacgg
     3481 ccaagcggcg ctgcaccagc tcctgctctg gaccgacgct cgctgggact tcgcgcacga
     3541 ggacatcgtc cgtcgccagc aaatcccgct ctcgcccgac gagctattcg ccgatgccga
     3601 gcgattcctc gaaggcgtgc gcgaatccgc aggtcacctc tcgccggcga cggtgctcga
     3661 gcaagacgtc cagcgcatcc aagacctcgg caagcaaatc ccgacggagg tccacggcgt
     3721 ccttcgcatg ttcgacggcc accgcgtgct caccgatgtg ctcgaggact cgccgtatcg
     3781 cgtgttcgag acgctgcgcg tggctcaaaa ggcggtcgaa gcgggcctcc tccgcaaagt
     3841 cgactcgcag cgcccgaaag caacgtggcg cgccgtgctc gcgatcgacg agtggctcgt
     3901 cggcagcgaa acgcgtgcag ccgtcgtcga gcgcaccgcc gacctcaccg acagcgagcc
     3961 cgtcggcaag aagaaggccg gcaaaggcaa ggcacgcgcg aagcgccgca aagccgcgac
     4021 gcccgtcgca ggcgtcaaag ccgagatcga ttggggcgcg ctcgtcccgc gcgtgatcgg
     4081 cgcagaggtc ggcccgctct cgggcgtagt gccggcgcaa gcgacggcgg gcgaagtcga
     4141 gctgccgacg cgcgaccgtg cgcgcgaaaa gctcgaggcg ctgatggaca cggacaagcg
     4201 ccaaaagatc ttcccgacgg agatcgggct cgagccgaag gtcgtgatcg acagcggcat
     4261 gggcggcgat accgacgagt gggagcgcat cgagctcgaa gcgaagaaga agctggcagt
     4321 cgccgaggcg tcgaacgagc cggagaccct cggctcgcca aagcgcaaga aacgcaagca
     4381 agaccgcctc gccgcggctg ccgcggacaa ggcctccgcg gagaaggcgc ctgccgaaaa
     4441 ggccgacgcg gagaatacgg ctgccgaaca cgcggcgcct cgacaggcga cgacccaaca
     4501 agcgacgacc caacaagcgg cgacccaaca ggcgacgact caacaggcga cgacccaaca
     4561 cgcgacgacc caacaggcga cgccccaaca ggcgacgccc caacaggcga cgacccaaca
     4621 ggcgacgacc caacaagccg cctcgcagac gatcgctgct gacagagggc cggccgtcga
     4681 cgaagtcgcc gcggcagcga agctcgtcgc cgagaaagcc gccgcggatg caaaggctgc
     4741 ggccgagcgc gcgtcggtgc acgccaacgc ggccgacaac gcgcgcgaag cagccgcccg
     4801 cgccgccgca gaagcaagcg ccgcgacgac ggcggtcgcc gcggccgagc acgccgccac
     4861 cgtcgggaaa gccgcagccg acgcgcgcgc cgcgtccgat cgcgcggcga ccgacgcgaa
     4921 agccgccgcc gagttcgccg cagccgcctc gcgcgccgcc gtcgactcga tggctgcagc
     4981 gcacgccaaa gcggccgtcg agcgatccgc cgccgacgct gcagccgccg cggaagccag
     5041 cgaagccgcc gcgcgcctcg ccgccgatgc aaaggccgcc gtcgacgcga ggctcgccgc
     5101 cgacgcgaaa gtcatcgccg aagccaaagc caccgcggaa gcaaacctcg tcggcgcacg
     5161 caccgaggcc gaccgcgcat cgatggccgc gagcgccgcc gacaacgctc gtgccgccgc
     5221 ggaacgcgcc gctgccgatg cggccgcgcc aatgaccgat gcgacatcgt ccaacgacta
     5281 cgccgtcgcc gccgccgccg ctgcgtccga cgcgcgcacc gccgcaaatc gcgttgcatc
     5341 gcacgcaaag accgccgcgg agttcgctgc tgccgccgcg cgcgcagccg gcgactcgat
     5401 ggcgggcgcc cacgcaaagg ccgctgccga gcgcgccgcc accgacgctg ccgccgccgc
     5461 cgatgcaagc cgcgccgccg aacgcgctgc cgccg
//
LOCUS       DATLBZ010000541        13070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5917007, whole genome shotgun sequence.
ACCESSION   DATLBZ010000541 DATLBZ010000000
VERSION     DATLBZ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13070
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5917007"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VMZ53_27070"
     CDS             <1..984
                     /locus_tag="VMZ53_27070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32203.1"
                     /translation="ENGMAVAESISKFGTAPEFMYGKVTTPEWLTLGGDLRGASGYMQ
                     APQRYLVTFPMQTDLYAVAQKGNIDVAITIGYRPPEEGNEALTSVWSREHYVRWEQDG
                     STHQGVSVRVGRFMPVFGLRFAEHPIYTRRYGGTALYSDTYAAEVGFVSDKLEAHATG
                     FIKDPLIDPVRHANGAAAYAELHVAEHTIVGAGGMAEIGGTHDGLDYGSRFRGEISAK
                     QYLAGPDVLVQAELQIANPHVDGYGYKQIIGYVMGTWFGRHGVMVDLGVGHYDENIGL
                     HGLDRDCVDLNVHWFATSHVELNVTNRVELIGKGGGGPTGAYSLLMAHYRL"
     gene            1006..2157
                     /locus_tag="VMZ53_27075"
     CDS             1006..2157
                     /locus_tag="VMZ53_27075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32204.1"
                     /translation="MTRSLALIVALVPAVAAAQSDPAGAPAADPVPAPAPAAAPDSPP
                     APDATPAFTWEPFGFLRLQYIVVQNDPNVAFVGRDDGFELQNARAGFRGKLAERASFV
                     LSIDGAVDERAQQNTPQGKLRVGLRDAYGDVIAGKLVVRGGYFETWVDPQALVADTSR
                     EYVDKPLESRGMRSTEGWYTPGLPPGRSLGAALLLEPAEANQIGFEVAVQNGSDEFSS
                     NNDNDKPAVSASAMFRLADDGFVVASGRWNPRTVGELPFRQDETDLQGSIGAQLNVGL
                     ASFGAGAIVQRTTFESTNGPVQNAYGAHAQFMVTAPAKVPLGFGYRFGILDPSSLILT
                     DRVMEHTVGSVISVPSYRMRVQLQITHVMEQGARDLSNSRAQAAVELQL"
     gene            2154..5231
                     /locus_tag="VMZ53_27080"
     CDS             2154..5231
                     /locus_tag="VMZ53_27080"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HUS32205.1"
                     /translation="MKTALLLLLAACTGSAAPHARAQRIGKLQEAIGGPHAIGRIGDF
                     LLENDQVRFVIADTGRCSPGQDNCKETFGRVNTTFGGTLVDADLQRVQGDGEGNDQLA
                     ELLPAFFFTAIDPTKVEILADGSDGGAAIVQVTGTAGDLFQTVGHINAAIVGPMLEYR
                     QTYKLEPGARYVTIDTTIKNISSGMHPFPYLDPSQLSNLLGTDIPGIGNLQLSVPVGQ
                     LPLFGGEQSIFAPGVAGFNVLFAIQDSYKMAGGFPSFPGMVVDFVATKGNGVSYGLAL
                     PESPDNYVNAYPNGYAGQDITKYSMVLPFSYAGVTGAYQYRAPPILAAGAEKTYTSYF
                     VVGRGDVGSIYDTILELRKEKTGSFGGRVVDELSQQPIAGADVIISKAGKVLDQLETD
                     ARGSFLGKLPVGDYTYRVVTNDRPLTDEKMIHIDEGGQIGRLLQVQPPATLAISVIDE
                     VGRHAPAKIQLIGHDARVKTADGRGILYSLQLGEAVRPTAYDGTDRFIEKAWWTADGR
                     LDAKVRPGTYDLVISRGPEYELTTETITLEAGGLVGKQLQLKRAFSTDGWIAGDFHIH
                     SQPSTDSGLPISERVASCAAEGLEVAVATDHNVITDYAPVIAETGLDPWLFGIPGMEL
                     TTFETGHFIAYPLKVDPGSTRGGEFLWAHQTPKSMFDQLRALATSRDESVVQIAHPRQ
                     QVLGYFAQYFLDVATAEPYTPTGIMGLFAPYGDEFRGENFSYDFDALELLTAAKYEDV
                     HTFRAPNPLPPGPFPDPQPVPGQVVVGKDGKPTFPGTIETWFTLLDRGHKATGMGASD
                     AHHLLGDEPGSARTLLYVGPGRDTLGGFTRGDVTTALKEHRAITTNAPFIEMTVNGQG
                     VGSTVTASGSVPVEIHVRAPSWAAVDRLKVYTNGGTVVTDMAITTGTEFKTTVMITPT
                     TDAWVVAEVTGTANMFPAFSAVEFPPFDATIIIGALSAGFNLSSLPIASTLKPNRQHI
                     AQPYAITNPIWIDRDGNGWTPPKSPLPNKFAPRAADKTPDVRALFDALPEVSQ"
     gene            5228..5812
                     /locus_tag="VMZ53_27085"
     CDS             5228..5812
                     /locus_tag="VMZ53_27085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32206.1"
                     /translation="MSWLTRCVAIALAICLAGQFAWGHTFPPVHTVVIQVEGCEIAML
                     VGYRPASGEATDTLLARIAAEPKSHQLEAAKAALAREALAPFSVTVDGKPLSATSLQA
                     KIGIDPGGTRPMVVVLVTYAIPAGGTLAVSSREPRTTRISWTDRASQRVDLDHAPGQG
                     KWFTSVASFLLSLTPPTGVSACANSNTSDSSHSR"
     gene            5770..7425
                     /locus_tag="VMZ53_27090"
     CDS             5770..7425
                     /locus_tag="VMZ53_27090"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS32207.1"
                     /translation="MRQLKYVGLVALALTACADNAYDPDAPAIDPNAPRVHITSPARG
                     TIAGDVQKIEVKGTATDDSGVDSVLVNGVAAQVAADGTFTAYVPLVPGTNLIHAIAKD
                     KQGNEGKESRAVVAGPMATLARHIPESITATLSAQTFDAIGRGVAGFLKNGDLMTTMQ
                     GMNPVVNSGAPDGPDCLYAQAFITSMSVQDADILMSPQNGGVFLSGELTNVRVGMHLQ
                     WAVACADGSRDVVISASKVSISGMLNIGLAGRNFDIKLVTPNVTITGFNLNLGGVPQT
                     IIDMLNLNSVLGPVLAWGLEKIAVPVMNKSLAGLNDTKTIDVLGTKVDIDVKPSHITF
                     TREGGTVLLDTSLRAHNDSGTMVFVPNTVPDMDMSQGFQLAVADDAANQLLTSMWSAK
                     GMDTTIDLKNGSYGEVGTLYDSVQLEVKVPPYVDAASDKLVLTVGDMIANFKLGGSPT
                     AAVAINAQVEMKVAKGTDGKFRLDVGTPTTYVDILDENIEGANQLSNAQFEAIASFAL
                     SRVVSVGSGAVGAIPLPQFGGVGVTQLSIDSKHGYLVVNGEIQ"
     gene            complement(7406..8647)
                     /locus_tag="VMZ53_27095"
     CDS             complement(7406..8647)
                     /locus_tag="VMZ53_27095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS32208.1"
                     /translation="MVSRPTAWSVAIVAMLTMTVSYIDRSTLAVLADSVTKALHITET
                     QYGWLQSAFSIAYLVATPLSGWWIDRIGARRGLVASILVWTAVSALQAIAPGFGALFI
                     LRIALGLAEGPSFPGAAQTNQRILPPGERARGFGVLFTGSSIGGMIAPQLASYLFRVT
                     GSWRIAFAGSALVGLVWIPLWIAVTWRRDVRAQLDVPPPPREEPRSLGPRIGGALREY
                     VELLKHPNMIRALIAILGAAPVIGFVLLWAAKYLGRAWHVEQGDIGHYLWLPPLIFDV
                     ATIAFGDLASRRKRPDGAPPRLLFAIGVLLAATLAFLPLADTRWHAVVQMGIAMAGGG
                     IIYMLVTTDLLSRMPPHRTALAGGTVASGQSFALIVTSPLIGMSVDRFHSFDVAVVVL
                     GLWMLPCGAYWILKRPTESRH"
     gene            complement(8665..9777)
                     /locus_tag="VMZ53_27100"
     CDS             complement(8665..9777)
                     /locus_tag="VMZ53_27100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32209.1"
                     /translation="MKGSVGASAILALAAAAAAGCGEKTVELTMVLPSSNVAAQYDTS
                     CVKAVQLYLNGTTYPDNVDDYELDCQDVEMPAGTFAGVHDEIAGKFALNFPASGLSGV
                     EVYAYSGPCDAPSPQEFDLVFYGSSPYIGQDPVEVAITPNLSCTPNTVTVRPIDILKL
                     SATKQCAQAAWTTGKLGLSTLSPLPFTDESYWWGGLSSTAIVGGTATITAPTTVAPKT
                     CLAVALYTNDWAEVTCVPPQDQRACAGGSELEAPMINVDVGDMSINPNHFTQYGGVIY
                     GVVYGAQPIAGAKVELDPADADIGKVVYLDMPAGVENGMGALTEVNGATGTNTTGLFA
                     IYTQSLVHVTVTANGQSVKRVVGANEDEISAVVVKL"
     gene            complement(9793..10659)
                     /locus_tag="VMZ53_27105"
     CDS             complement(9793..10659)
                     /locus_tag="VMZ53_27105"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS32210.1"
                     /translation="MELEPHAVLVWSGTKPRREIFPIPPSGLVVGRDLFETDDERISR
                     QHTRITPVPNGYRLEDLKTRHGTFVEGNAPLHEPFVVTKSPRFFRAARSVFILTRDVE
                     AHGVLIDLPPSLLRMVRARSTQGQLHASAVIDSLVEARRSGLEHVLDMFARSVAAWIP
                     RGRSLRADELWLTSPPSAAPGSKIRAFPTREDRATCMLLDKLEKEMGITLGGEMRPHG
                     QGFVWGDQGDKGKRRFEVDCTWGNTGASEGPAYNIHLFDGKQELPRSTLRKLDDAVAI
                     VKAWIADGRLLQ"
     gene            complement(10664..>13070)
                     /locus_tag="VMZ53_27110"
     CDS             complement(10664..>13070)
                     /locus_tag="VMZ53_27110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32211.1"
                     /translation="MSVGIRPQSPPTITLGHNTFKDDGMGGKVLDEPLLDIRFSALEI
                     DFFASIDDQYNRVFTVVTDVHLPIGLQTTAMGELTPVIGNPMDAFTNVSVKNSDAITE
                     SPAEIAGLFPTLLGLVLPQLSGGLGSIALPELGGLKLSVTSVTAIDNNNFLAIFINFV
                     KATMPRRVNTHVEILGIAEPPDAVARDAAQWTTHRPPAVTLGLSGDAPDLEHSYRIDA
                     GSWSAWSPAAQQTLAPRVFWLPGVHIVEVRSRQIGHPETISLPEKLELPLGTGVPLEH
                     GGTVVQRNADFHGQAGAAGCSCDSRGGSPANGALLALVIGFVLLPMRRVRRRLAHFAR
                     EAMRLGPLVWLAALALLPACNCGAPCGDADCIAGDIDPGALGRFTSIAADDKRVLVAT
                     YDQVYGDLVVVDATNPSSLKLKSVDGVPDSTPTHDPDSSYRGGVEDPGINVGAWTSIA
                     LSGVAHVSYQDRDEGLLKYAFETKAGGSWKSYALDTSDTENGLYTSIAIDGDKKPAIA
                     YLALGIDDGMGHRNSELRLVRANGPTPNDASDWTSTLLATGPASCGGLCGADKCVEGA
                     DATAPQECVSPTTNCTPACASTDACVAGTCRPIVEAPKVLLPPGGAGAYVSLVALPDG
                     RLAAVYYNANKRALVLAIEGNKNTNDFTDVILDGDAVGADRGMWCSATVATDGTIHIA
                     YQDALGDQLMYTSWNGSPGTPVVVDDGQRSGDRTHPVGSGSSIYLVGGEPAIAYQDGL
                     TSDVYLATRSAGAWTTNGLAMGPLLDGFSIGATSGHGTPVLAWGQMDPAKAPPPNLIV
                     RTP"
BASE COUNT         2268 a         4304 c         4350 g         2148 t
ORIGIN      
        1 gagaacggca tggccgtcgc cgagtcgatc tcgaagttcg ggaccgcgcc cgagttcatg
       61 tacggcaagg tcacgacgcc cgagtggctg acgctcggcg gtgatctgcg cggcgcatcg
      121 ggctacatgc aggcgccgca gcgctacctc gtgacgttcc cgatgcagac ggacctctac
      181 gcggtcgcgc agaagggcaa catcgacgtc gcgatcacga tcggctatcg cccgcccgag
      241 gaaggcaacg aggcgctgac gtcggtctgg tcgcgcgagc actacgtgcg ctgggagcag
      301 gacggctcga cgcatcaagg cgtcagcgtg cgcgtcggtc gcttcatgcc ggtgttcggc
      361 ctgcggttcg ccgagcaccc gatctacacg cgtcgctacg gcggcacggc gctctactcg
      421 gatacgtacg cggcggaagt cgggttcgtc agcgacaagc tcgaggcgca cgcgaccggc
      481 ttcatcaaag atccgctcat cgatccggtg cgccacgcca acggtgccgc ggcgtatgca
      541 gagctccacg tcgccgagca cacgatcgtc ggcgcgggcg gtatggcgga gatcggtggc
      601 acgcacgacg gtctcgatta cggcagcaga tttcgcggcg agatcagcgc gaagcagtac
      661 ctcgcaggtc ccgacgtgct cgtgcaggcc gagctccaga tcgcgaatcc gcacgtcgac
      721 ggctacggct acaagcagat cattggctac gtgatgggga cctggttcgg ccggcatggt
      781 gtgatggtcg acctcggcgt tggccactac gacgagaaca tcggcttgca cgggctcgat
      841 cgagattgcg tcgatctgaa cgtccactgg ttcgcgacgt cgcacgtcga gctcaacgtt
      901 acgaaccgcg tcgagctgat cggcaaaggc ggtggtggac ccaccggtgc gtactcgctg
      961 ctgatggcgc actaccgcct gtagagcgtg tacgatcgcg gctacgtgac tcgtagccta
     1021 gctctgatcg tcgcgctcgt tcctgcggtc gcggcagcgc agtctgatcc agctggtgct
     1081 ccagcagcgg atccggttcc ggctcccgct cctgcagcgg ctcccgattc gcctcctgca
     1141 ccggatgcaa cgccggcgtt cacgtgggag ccgttcggct tcttgcgcct gcagtacatc
     1201 gtcgtgcaga acgatccgaa cgtcgcattc gtcggccgcg acgacgggtt cgagctgcag
     1261 aacgcgcgcg caggatttcg cggcaagctc gccgagcgcg cgagcttcgt gctgtcgatc
     1321 gacggcgccg tcgacgagcg cgcgcaacag aacacgccgc agggcaagct gcgcgtcggg
     1381 cttcgcgatg cctacggcga tgtcatcgcc ggcaagctcg tcgtgcgtgg cggctacttc
     1441 gagacgtggg tcgatccgca ggcgctcgtg gccgacacgt cgcgcgagta cgtcgacaag
     1501 ccgctcgaat cgcgcggcat gcgatcaaca gaaggctggt acacaccggg actgccgccc
     1561 gggcggtcgc tcggcgcagc gctgctcctc gagccggcag aggcaaatca gatcggcttc
     1621 gaggtcgccg tgcagaacgg ctccgacgag ttctcgtcga acaacgacaa cgacaagccc
     1681 gcggtctccg cgtcggcgat gttccgcctc gccgatgatg gattcgtcgt cgcgagcggg
     1741 cggtggaatc cgcggaccgt cggcgagctg ccgtttcgcc aagacgagac cgatctgcag
     1801 ggctcgattg gtgcgcagct caatgtcggc ctcgcatcgt ttggtgcggg cgcgatcgtt
     1861 cagcgcacga cgttcgagtc gacgaatggc ccggtgcaaa acgcgtatgg tgcacacgcg
     1921 cagttcatgg tgaccgcgcc tgcgaaagtt ccgctcggct tcggctaccg cttcggcatt
     1981 ctcgatccgt cgagcttgat cctgacggac cgcgtgatgg agcacacggt gggatccgtg
     2041 atcagcgtgc catcgtatcg aatgcgcgtg cagctacaga tcacccacgt gatggagcag
     2101 ggcgctcgcg atctatcgaa cagtcgcgcg caggctgcgg tggagctgca gctttgaaaa
     2161 ctgcgctctt gctgctgctc gcggcttgca ccggctcggc ggcgccgcac gcgcgcgcgc
     2221 agcgcatcgg taagctgcaa gaagcgatcg gcgggccgca cgcgatcggg cgcatcggcg
     2281 attttctcct cgagaacgat caagttcgct tcgtgatcgc ggataccggc cgctgctcgc
     2341 cgggtcagga caactgcaag gagaccttcg gccgcgtcaa cacgacgttc ggcggcacgc
     2401 tcgtcgatgc ggatctgcag cgcgtgcaag gcgatggcga gggcaacgat cagctcgcgg
     2461 agctactgcc cgcgttcttc ttcacggcga tcgatccgac gaaggtcgag atcctcgccg
     2521 atggctccga tggcggcgcg gcgatcgtgc aggtcaccgg caccgccggc gatctgtttc
     2581 agaccgtcgg ccacatcaac gccgcgatcg tcgggccgat gctcgagtat cgccagacgt
     2641 acaagctcga gcccggcgcg cgctacgtca cgatcgatac gacgatcaag aacatctcga
     2701 gcgggatgca tccgttcccg tatctcgatc cgtcgcagct ctccaacctg ctcggcaccg
     2761 acatccccgg catcggcaac ctccagctca gtgtccccgt cggtcagctg ccgctgttcg
     2821 gcggcgagca atcgatcttc gcgcccggcg tcgcgggctt caacgtgctg ttcgcgatcc
     2881 aagacagcta caagatggcc ggcggctttc cgtcgttccc cggcatggtc gtcgacttcg
     2941 tagcgacgaa gggcaacggc gtctcgtacg ggctcgcgct gcccgaatcg ccggacaact
     3001 acgtcaacgc gtatcccaat ggctacgccg ggcaagacat cacgaagtat tcgatggtgc
     3061 tgccgttctc gtatgcgggc gtgaccggcg catatcagta ccgagctccg ccgatcctcg
     3121 ctgcgggcgc cgagaagaca tacacatcgt atttcgtcgt cgggcgcggc gatgtcggaa
     3181 gcatctacga cacgatcctc gagctgcgca aagagaagac gggcagcttc ggcggccgcg
     3241 tggtcgacga gctctcgcaa cagccgatcg caggcgcgga tgtgatcatc tcgaaggccg
     3301 gcaaggtcct cgatcagctc gagaccgacg cgcgcggctc gttcctcggc aagcttcccg
     3361 tcggcgacta cacgtatcgc gtcgtcacga acgatcggcc gctcaccgac gagaagatga
     3421 tccacatcga tgaaggtggg cagatcgggc gcctcctgca ggtgcagcca cccgcaacgc
     3481 tcgcgatctc cgtgatcgac gaggtcggcc gccacgcgcc cgcgaagatc cagctcatcg
     3541 gtcacgacgc acgcgtgaag acggctgatg ggcgcggcat cctgtactcg ctgcagctcg
     3601 gcgaagcggt gcgtccgacc gcgtacgacg gcaccgatcg cttcatcgag aaggcatggt
     3661 ggaccgcaga tggccggctc gacgcgaagg tgcggcccgg aacgtacgac ctcgtgatct
     3721 cgcgcgggcc cgagtacgag ctcacgacgg agacgatcac gctcgaggcc ggcggtctcg
     3781 tcggcaagca gctgcagctg aagcgcgcct tctcgacgga cggttggatc gccggcgact
     3841 tccacattca cagccagccg tcgacagact cggggcttcc gatctccgag cgcgttgcga
     3901 gctgcgcggc ggaaggcctc gaggttgcgg tcgcgaccga ccacaacgtc atcacggact
     3961 acgcgcccgt gatcgcggag accggcctcg atccgtggct gttcggcatc ccaggcatgg
     4021 agctgacgac gttcgagacc ggccacttca tcgcgtatcc gctgaaggtc gatccgggct
     4081 cgacgcgtgg tggcgaattc ttgtgggcgc atcagacgcc gaagtcgatg ttcgatcagt
     4141 tgcgcgcgct ggcgacgagc cgcgacgagt cggtcgtgca gatcgcgcat cctcgccagc
     4201 aagtgctcgg ctacttcgcg cagtacttcc tcgacgtagc gacggcggag ccgtacacgc
     4261 ccactgggat catgggcctg ttcgcgccgt acggcgacga gttccgcggc gagaacttca
     4321 gctacgactt cgacgctctc gagctgctca ccgcagcgaa gtacgaagac gtccacacgt
     4381 tccgcgcgcc gaatccgctg ccgcccgggc cgtttcccga tccgcagccg gtgccgggcc
     4441 aagtcgtcgt cggcaaagat ggcaagccga cgtttccggg cacgatcgag acgtggttca
     4501 cgctgctcga tcgcggtcac aaggcgaccg gcatgggcgc atccgacgca caccacttgc
     4561 ttggcgacga gcccggcagc gcacgcacgc tgctctacgt cggtcccggt cgcgacacgc
     4621 tcggcggttt cacgcgcggc gatgtgacca ccgcgctaaa agagcaccgc gcgattacaa
     4681 cgaacgcgcc gttcatcgag atgaccgtga acggacaggg cgttggctcc accgtcacgg
     4741 cgagtggcag cgtgccggtc gagattcacg tgcgcgcgcc gagctgggcg gcggtcgatc
     4801 ggctgaaggt ctacacgaac ggcggcaccg tggtcaccga catggcgatc accaccggca
     4861 ccgaattcaa gacgacggtg atgatcacgc cgacgaccga cgcgtgggtc gtcgcggagg
     4921 tcacgggcac cgcgaacatg ttccccgcat tctcggcggt cgagttcccg ccgttcgatg
     4981 caacgatcat catcggcgcg ctgtccgcgg gcttcaatct gtcgagcttg ccgatcgcgt
     5041 cgacgctcaa gccgaatcgc cagcacatcg cgcagcccta tgcgatcacg aatccgatct
     5101 ggatcgatcg cgatggcaac gggtggacgc cgccgaagtc cccgctgccg aacaaattcg
     5161 cgccgcgcgc ggcggacaaa acgcccgatg tgagggccct cttcgacgcg cttcccgagg
     5221 tgtcgcaatg agctggctga cgcgctgcgt ggctattgct ctagccatct gcctggcagg
     5281 ccaattcgcc tgggggcata ccttcccgcc agtgcacacc gtagtcatcc aagtcgaagg
     5341 ctgcgaaatc gcgatgctcg tcggctaccg gccagcgagc ggcgaagcga ccgacaccct
     5401 cctcgcgcgc atcgctgcgg agcccaaatc gcaccaactc gaggctgcca aagccgctct
     5461 ggcgcgcgaa gcgctggccc cgttctcggt gactgtcgac ggcaaaccgc tgtctgcgac
     5521 gtccctgcaa gcgaagatcg gcatcgatcc aggcggcacc cggccgatgg tcgtcgtgct
     5581 cgtgacctat gcgattcccg cgggtggcac cctcgccgtg agctcgcgcg agccgcgtac
     5641 cacgcggatc tcttggaccg atcgagccag ccaacgggtc gatctcgatc acgccccggg
     5701 acaggggaag tggttcacca gcgtggcctc cttcttgctg agtctgactc caccaacggg
     5761 agtctcagca tgcgccaact caaatacgtc ggactcgtcg cactcgcgct gaccgcttgc
     5821 gcggacaacg cctacgaccc agacgcgcct gcaatcgatc cgaacgctcc ccgcgtgcac
     5881 atcacgtcgc ccgctcgcgg cacgatcgcc ggtgacgtcc agaagatcga agtcaaaggc
     5941 acggcgaccg atgactccgg cgtcgactcg gtgctcgtca atggtgtcgc ggcgcaggtc
     6001 gcggcagacg gcacgttcac tgcgtacgtt cctctcgttc caggcacgaa cctcatccac
     6061 gcgatcgcga aggacaagca aggcaacgag ggcaaggaat cgcgcgcggt cgtcgcaggt
     6121 ccgatggcga cgctcgctcg tcacatcccc gaatcgatca cggcgacgct gtccgcgcag
     6181 acattcgatg cgatcggccg cggcgtcgca ggctttctga agaacggcga cctcatgacg
     6241 acgatgcagg gcatgaaccc ggtcgtcaac agcggcgcgc ccgacggtcc cgactgcctc
     6301 tacgcgcaag cattcatcac gagcatgtcc gtccaggatg ccgacatcct gatgagcccg
     6361 cagaacggcg gcgtcttcct ctcgggcgag ctcacgaacg tgcgcgtcgg gatgcacctg
     6421 caatgggcgg tcgcctgtgc agatggctcg cgtgacgtag tcatctcggc gtcgaaggtc
     6481 tcgatcagcg gcatgctcaa catcggcctc gcaggtcgca acttcgacat caagctcgtc
     6541 acgccgaacg tgaccatcac cggcttcaac ctcaacctcg gtggcgtccc gcagacgatc
     6601 atcgacatgc tgaacctgaa ctcggtgctc ggtccggtcc tcgcgtgggg cctcgagaag
     6661 atcgcggtgc cggtgatgaa caagtcgctc gccggtctca acgatacgaa gacgatcgac
     6721 gtgctcggca cgaaggtcga catcgacgtg aagccctcgc acatcacgtt cacgcgcgaa
     6781 ggcggtacgg tcctcctcga tacgtcgctg cgcgcccaca acgacagcgg cacgatggtc
     6841 ttcgtcccga acacggtgcc cgatatggac atgtcgcaag gcttccagct cgcggtggcc
     6901 gacgacgcag cgaaccagct gctcaccagc atgtggtcgg cgaagggcat ggacacgacg
     6961 atcgatctca agaacggctc gtacggcgaa gtcggcacgc tctacgactc ggtgcagctc
     7021 gaagtgaagg ttccgcccta cgtcgacgcc gcgagtgaca agctcgtgct cacggtcggc
     7081 gacatgatcg cgaacttcaa gcttggcggt tcgccgacgg cagccgtcgc gatcaacgcg
     7141 caggtcgaga tgaaggttgc caagggcacc gacggcaagt tccgcctcga tgtcggcacg
     7201 ccgacgacgt acgtcgacat cctcgacgag aacatcgagg gagcgaacca actctcgaac
     7261 gcgcagttcg aagcgattgc ctcgttcgcg ctatcgcgcg tcgtctcggt tggctcgggt
     7321 gcggtcggcg cgattccgct gccgcagttc ggcggcgtcg gtgtcacgca gctgtcgatc
     7381 gacagcaagc acggctacct cgtcgtcaat ggcgagattc agtaggtcgc ttcaagatcc
     7441 agtacgcgcc gcatggcagc atccatagac cgagcacgac gacggcgacg tcgaagctgt
     7501 ggaagcgatc gacggacatg ccgatcagcg ggctcgtcac gatgagcgcg aatgattgcc
     7561 ccgacgcgac ggttccgccg gcaagcgcgg tgcggtgcgg tggcatgcgc gagagtaggt
     7621 cggtcgtcac gagcatgtag atgatgccgc cgcccgccat cgcgatgccc atctggacga
     7681 cggcgtgcca gcgcgtatcg gcaagcggaa gaaatgcgag agtcgccgcg agtaggacgc
     7741 cgatcgcgaa gaggaggcgc ggcggtgcac cgtccggccg cttccgccgc gacgcgagat
     7801 cgccgaacgc gatcgttgcc acgtcgaaga tgagcggcgg gagccacagg tagtggccga
     7861 tatcgccttg ctcgacgtgc caggcacgcc cgagatactt cgcggcccac agcagaacga
     7921 agccgatgac cggggcggcg ccgaggatcg cgatcagcgc gcggatcatg ttcggatgtt
     7981 tgagcagctc gacgtactcg cgcagggcac cgccgatgcg cggcccgagc gagcgcggct
     8041 cctcgcgcgg tggtggaggc acatcgagct gcgcgcgaac atcgcgtcgc cacgtgacgg
     8101 cgatccacag cgggatccag acgagaccga cgagcgcgct gcccgcgaat gcgatgcgcc
     8161 acgagcccgt gacgcgaaat agatagctcg cgagctgcgg cgcgatcatg ccgccgatcg
     8221 acgagccggt gaagagcacg ccgaagcccc gcgcgcgctc acccggcgga agaatgcgct
     8281 gattcgtctg ggccgcgccg ggaaagctcg ggccttccgc gagaccgagc gcgatgcgca
     8341 ggataaacag cgcgccaaag cccggcgcga tcgcctgcag cgcggagacc gcggtccaca
     8401 cgagaatcga agcgacgagg ccgcggcgcg cgccgatgcg atcgatccac cagcccgaca
     8461 gcggcgtcgc gacgaggtac gcgatcgaga acgcggactg cagccagccg tactgcgtct
     8521 cggtgatgtg gagcgccttc gtgacgctgt ccgcgagcac ggcgagcgtc gagcggtcga
     8581 tgtagctgac cgtcatcgtc agcatcgcga cgatcgcgac agaccacgcg gtgggcctcg
     8641 acaccatcgg cgcagcgtat ccgatcagag cttgacgacg acggccgaga tctcgtcttc
     8701 gttcgcgccg acgacgcgct tcacgctctg gccattcgcc gtcaccgtca cgtggacgag
     8761 tgactgcgta tagatcgcga agaggcccgt cgtgttcgtg ccggtcgcgc catttacttc
     8821 ggtgagcgcg cccatcccgt tctcgacgcc ggcgggcatg tcgagataga cgaccttacc
     8881 gatatcggca tccgccggat cgagctcgac cttcgcgccc gcgatcggct gcgcgccata
     8941 cacgacgccg tagatgacgc cgccgtactg cgtgaagtgg ttcggattga tcgacatgtc
     9001 gccgacgtcg acgttgatca tcggcgcttc gagctcgctg ccgcccgcgc acgcgcgctg
     9061 atcctgcggc gggacgcacg tgacttccgc ccagtcgttc gtgtacagcg caacggcgag
     9121 acacgtcttc ggcgcgaccg tcgtgggcgc ggtgatcgtg gccgtgccac cgacgatcgc
     9181 ggtcgatgac aggccgcccc accaatacga ctcgtcggtg aacggcagcg gcgacagcgt
     9241 cgacaagccg agcttgccgg tcgtccacgc ggcctgcgca cactgcttcg tcgccgacag
     9301 cttcaagata tcgatcggcc gcaccgtcac cgtgttcggc gtgcagctga ggttcggcgt
     9361 gatcgcgacc tcgacgggat cctggccgat gtacggcgag cttccataga acacgagatc
     9421 gaactcctgc gggctcggcg cgtcgcacgg cccggagtac gcgtacacct cgacgccgga
     9481 caaccccgac gccggaaagt tcagcgcgaa cttccccgca atctcatcgt gcacgcccgc
     9541 aaacgtcccc gcgggcatct cgacgtcctg acagtcgagc tcgtagtcat ccacgttgtc
     9601 gggatacgtc gtgccattca agtacagctg cacggccttc acgcacgacg tgtcgtactg
     9661 cgccgcaacg ttgctcgacg gcagcaccat cgtcagctcg acggtcttct cgccgcatcc
     9721 cgccgctgct gctgctgcga gcgcgaggat cgcgctggcg ccgacgctac ccttcactcc
     9781 gccgatctta cctcactgca gcaggcgccc atccgctatc catgccttca cgatcgcgac
     9841 agcgtcgtcc aatttgcgca acgtgctccg cggaagctcc tgtttcccgt cgaagagatg
     9901 aatgttgtac gcgggtccct cactggcccc cgtgttgccc cacgtgcaat ccacctcgaa
     9961 gcggcgcttg cccttgtcgc cctggtcgcc ccacacaaag ccctgtccat gcggccgcat
    10021 ctcgccgccg agcgtgattc ccatctcctt ctcgagcttg tcgagcagca tgcacgtcgc
    10081 gcgatcctcg cgcgtgggaa acgcgcggat cttgctgccg ggcgctgccg acggaggcga
    10141 cgtcagccac agctcgtccg cacgcagcga ccgccctcgc gggatccacg ccgccacact
    10201 gcgcgcgaac atgtcgagca catgctcgag gccactgcgc ctcgcctcga cgagcgaatc
    10261 gatcacggcg ctcgcgtgca actgcccctg cgtgctgcgc gctcgcacca tgcgcaacag
    10321 cgacggcggc agatcgatca acacgccatg cgcttcgaca tcccgcgtga gaatgaacac
    10381 gctgcgcgcc gctcgaaaaa accgcggcga cttcgtgacg acgaacggct cgtgcaacgg
    10441 cgcgttgccc tcgacgaacg tgccatggcg cgtcttcaaa tcctcgagcc gatagccatt
    10501 cggaacgggc gtgatgcgcg tgtgctgccg gctgattcgc tcgtcgtcgg tctcgaacag
    10561 atcgcggccg accacgagcc cgctcggcgg aatcggaaat atctcgcgcc gcggcttcgt
    10621 gccgctccac accagaacgg cgtgaggctc gagctccact cgattacggc gtgcgcacga
    10681 tgaggttcgg cggcggtgct ttcgcgggat ccatttggcc ccacgcgagc accggggtgc
    10741 cgtgacctga cgtcgcgccg atcgagaagc cgtcgaggag cggacccatc gcgaggccgt
    10801 tcgtcgtcca cgcgccggcc gagcgggtcg cgaggtagac gtcggacgtg aggccgtctt
    10861 ggtacgcgat cgcgggctcg ccaccgacga ggtagatcga cgagccggag ccgacggggt
    10921 gcgtgcgatc accggagcgt tgaccatcgt ctacgacgac gggcgtgccg ggcgagccgt
    10981 tccagctcgt gtacatcagc tggtcgccga gggcgtcttg gtaggcgatg tggatcgtgc
    11041 cgtcggtcgc gaccgttgcg ctgcaccaca tgccgcgatc ggcgccgacc gcgtcgccgt
    11101 cgaggatgac gtcggtgaag tcgttcgtgt tcttgttgcc ttcgatcgcg agcacgagcg
    11161 cgcgcttgtt cgcgttgtag tagacggcgg cgaggcggcc gtcgggcagg gcgacgaggc
    11221 tgacgtacgc gccggcgccg cccggcggga ggaggacctt gggcgcttcg acgatcggac
    11281 ggcaggtgcc tgcgacgcac gcgtcggtgg aggcgcatgc gggcgtgcag ttcgtcgtgg
    11341 gcgagacgca ctcttgcggc gccgtcgcgt cggcaccttc gacgcatttg tccgcgccgc
    11401 acaggccgcc gcagcttgcc gggcccgtgg cgaggagcgt ggaggtccag tcggacgcgt
    11461 cgttcggcgt cgggccgttc gcgcgaacga gccggagctc gctgttgcgg tggcccatgc
    11521 cgtcgtcgat gccgagcgcg aggtaggcga tcgcgggctt cttgtcgccg tcgatcgcga
    11581 tcgaggtgta gaggccgttt tcggtgtcgg aggtgtcgag cgcgtacgac ttccagctgc
    11641 cgcccgcctt cgtttcgaac gcgtacttga ggaggccttc gtcgcgatct tgataggaga
    11701 cgtgcgcgac gccagacagc gcgatcgatg tccaggcacc gacgttgatg cccggatctt
    11761 cgacgccgcc gcgataggac gagtcggggt cgtgggtagg cgtcgagtcg gggacgccgt
    11821 cgacggactt cagcttcagc gaggatgggt ttgtcgcgtc gacgacgacg aggtcgccgt
    11881 agacttgatc gtaggtcgcg acgagcacgc gcttgtcgtc ggcggcgatc gacgtgaagc
    11941 gaccgagcgc gccgggatcg atgtcacccg cgatgcagtc ggcgtcgccg cacggtgcgc
    12001 cgcagttgca tgccgggaga agggcgagcg ccgcgagcca cacgagcggg ccgagacgca
    12061 tcgcttcgcg tgcgaagtga gcgaggcggc ggcggacgcg gcgcatcggg aggagcacga
    12121 agccgatcac gagcgcgagg agcgcgccgt ttgccggcga gccaccgcgc gagtcacagc
    12181 tgcagcccgc ggcgccggct tggccgtgga agtcggcgtt gcgctggacg acggtgccgc
    12241 cgtgctcgag gggaacgccc gtgccgagcg gcagttcgag cttttcgggg agcgagatcg
    12301 tttcggggtg gccgatctgg cgcgagcgca cctcgacgat gtggacaccc ggcagccaga
    12361 acacgcgcgg cgcgagcgtt tgctgcgcgg ccgggctcca tgccgaccac gagcctgcgt
    12421 cgatgcgata cgagtgttcg agatccggcg cgtcgccgga gaggccgagc gtgacggcgg
    12481 gcgggcggtg cgtggtccac tgcgcggcgt cgcgtgcaac ggcgtcgggc ggctccgcga
    12541 ttccgaggat ctcgacgtgg gtgttgaccc ggcgtggcat cgtcgctttg acgaagttga
    12601 tgaagatcgc caggaagttg ttgttgtcga tcgctgtgac ggaggtgacg gagagcttga
    12661 ggccaccgag ctcgggcagc gcgatcgagc cgaggccgcc ggagagctgc ggcaggacga
    12721 ggccgagcag cgtcgggaac aggcccgcga tctcggcagg cgattcggtg atcgcgtcgc
    12781 tgttcttcac cgagacattc gtgaacgcgt ccatcggatt gccgatgacc ggcgtgagct
    12841 cgcccatcgc ggtggtctgg agaccgatcg ggagatggac gtcggtgacg accgtgaaca
    12901 cgcggttgta ctggtcatcg atcgacgcga agaagtcgat ctcgagcgcg ctgaagcgga
    12961 tgtcgagcag tggttcgtcc agcaccttgc cgcccatacc gtcgtctttg aacgtgttgt
    13021 gaccgagcgt gatcgtcggc ggcgattgcg ggcgaatgcc gaccgacatc
//
LOCUS       DATLBZ010000542        15801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2198637, whole genome shotgun sequence.
ACCESSION   DATLBZ010000542 DATLBZ010000000
VERSION     DATLBZ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198637"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VMZ53_27115"
     CDS             <1..829
                     /locus_tag="VMZ53_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HUS32212.1"
                     /translation="ETLRSVSPAPDGETYAVVLTPGPYNSAYFEHSFLARTMGLELVQ
                     APDLFVDQDKVFCRTTRGPKRVDVIYRRTDEAFLDPEVFRADSVLGVPGLMRAYAAGN
                     VALANAPGNGVADDKAVYPFVPQMIEYYLGEQPLLEQVPTYVCLRDDDRKYVLEHLGE
                     MVVKAVDEAGGYGMLMGPQSTAEQRDEFRRRIIAEPRRYIAQHRVELSSCPTWVGDRH
                     RVEPRRVDLRPYILTSREGPWVLPGGLTRVALVEGSYVVNSSQGGGSKDTWVMKETT"
     gene            826..1794
                     /locus_tag="VMZ53_27120"
     CDS             826..1794
                     /locus_tag="VMZ53_27120"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HUS32213.1"
                     /translation="MISRVADHCFWFGRYVERAEATARMLSATANLALDAELPPAQVW
                     RPLLVVSGEEDEFRTRVAEDNDDHSAWGDGEIVQRFMVWDEANGVSLTRSIGGARWNA
                     RSIREVLSLEAWEAVNELHLHATSDEAQSQYTYHREAFYHQVRRSTQLALGLLRSTML
                     HDEPLDFIWLGVLLERVNQTARMLDVHHHSFTNLNGSKEIVETALWLSLLRGLSGSEA
                     YMKRAAGRISSDAVARFLISESAFPRSISYCVHSAYERLCGIRPPEQHALPGGISLER
                     LRVLDSWVAARSKDPLGGRSVHELLTHVIDEVHAICGTLGKELLGA"
     gene            1825..3576
                     /locus_tag="VMZ53_27125"
     CDS             1825..3576
                     /locus_tag="VMZ53_27125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32214.1"
                     /translation="MRVLGIAVVVVAGCGFEHGTLGESTTLVVDDSVEDFAAGELNDI
                     TVDKLGYLAPDAFALGGLHARAYGSPFLSDTSTWSNLQPMLTTPLGERYSSMPGSFAF
                     DRPYGLGLARTDNFTVVYDGELHLDAGTRTLQVTADDIALVELDLGGVRPAVFAHVSD
                     GQPARLTFEVPKAGWYPIRAALSEAGSDAFLRLALVEANTPRAVDPSELRARVTSAHG
                     VTVTASPDRVFAARAPDISIEPSLIDRTFGSAVPPYDFAGVSTVYYALRYAGQLRVET
                     TEARVFAFDLGTDTTRDYARLLIDGKPIAGRWPGLMDQLTSEPLELTPGWHDILLDYA
                     QSDATARLQLTMSSATRAATAIEPMVLRPVRDGGLLTSVYRNENINLVDGAALDVALK
                     LDAPAGATVQFLDAYFTLYSQTRTEMTASLEGPGGADPIMIPTTASYDMLYDYFPNRT
                     AAVNAPVDGTWHLVLTDNSPTTTGYIYYPALMATYRGGPDKPYAETMSYVSAPHDLRG
                     TLLAIRATGELDGAQLVIEARSGSETTLEAAPWQSPEATPPGELVQYRLTLTSDGWQY
                     PTIDRVELDIRRDVPAD"
     gene            3597..5318
                     /locus_tag="VMZ53_27130"
     CDS             3597..5318
                     /locus_tag="VMZ53_27130"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HUS32215.1"
                     /translation="MPNLLAMSFEGELAPSFTLHCLEPGHTLPDGWGLGYFPAGEPSA
                     AILKEPAPPQGSMRSQLALAWEQVASSLFMLHIRHATWGALSDANTQPFARTWGRRDW
                     MIAHAGSLDQRLPDLAGPFEPVGSTDTEEIFCQFLNKCVEKNWRSLTECDLDVITGWL
                     AELNGIGGMTLCLTDGRDLLVHADQRGEPLWIGELKPPYERIGFGDEDLGVDLTKRGA
                     KARKGVVISTEPQKPREGDAEKALTWRQLEPGQVLVVREGAIIAERKTGGAVEKPRNL
                     RLPAKAEPRTFDIVHKTIYRYAQAIERSQHVLRLTPCHDRLQTVRSHEINVSVPTGSR
                     EYEDVFGNRVRRLTVESPFTEMIIEAKSTVEVLDTDPLGFGPLKVSSTIPLVWMPWQR
                     QVLDPYLLPPELPETELSELVEYAMSFVKRNENDLLDTLLDINQTIFKEYAYTQGATT
                     VSTTAFEVYVNRRGVCQDFTNLMICLARLLGVPARYVCGYIYTGPKAANTAQSEASHA
                     WVQLYLPQVGWKGFDPTNGILTQTDHVRVAVGRNYLDATPTSGTIFVGGGGETLSVDV
                     RVEPHQG"
     gene            5331..6824
                     /locus_tag="VMZ53_27135"
     CDS             5331..6824
                     /locus_tag="VMZ53_27135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32216.1"
                     /translation="MVRHVALVALTVVAACGDDSGQGQPADASVDVAVDAAPRQIVAH
                     AVVRQIVNDASRSPVAGPEVLYKMSSAAQRTGERMDVTLPIGAAGDIGFATLDDRYRL
                     SVALDTTTPTEFQMRATQLELSGRFVGRYDRTPPVAGTTLAATITNAPGTGTAVITST
                     GVWTQTGRSGGTNGSFTIDWSTQAASLGGPIGLLDAAQNDRAYYIVLDVTGTAPQYAA
                     ISSSCSADITMTAGAPTAFTCTAAPLAQSSCMHAQLHFAGETARITAITGSLYPNYFN
                     NWLLQATATPSTGITSALLLAYQVPLASTTPSVDLDRNFTFGNPYPGTTPVLLMNTGK
                     SRSFALPGTNAMNISATVNVYLPAAPDCATVTEGPTGFVAIPSLPRLDGVHLDSDAVT
                     IGLASKLHTLTWDVASPGAADYWLVRVHRAIINGSNSTRLSVVRSYVTLDHDVVLDPA
                     DFTVGQTYFIEVQAVTGVPKAAMGDFREYTFPYATSSIFSSAFRITE"
     gene            6821..7900
                     /locus_tag="VMZ53_27140"
     CDS             6821..7900
                     /locus_tag="VMZ53_27140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HUS32217.1"
                     /translation="MNAPLNLKELETLAHARLAQLAWDYYSSGADDETCVKRNVEAFQ
                     QILLHYRVLVDVSTRDLSTTVLGHRVALPILVAPTAFHRLAHKDGELATVRAAGDAGT
                     IFTLSTLSNTAVESVVAAATGPVFFQLYVYKDRSATEALVRRVEAAGAKALVLTVDAP
                     LLGRRERDVRNNFALPSQLGVENLHAAGYAPVPRADRESGLAAYFADLLDPSLTWDAI
                     SWLRSITKLPVLVKGIVRADDAASAIEHGAAGIVVSNHGGRQLDASPATIDVLARCVD
                     AIAGRGEVLLDGGIRRGADVVKAIALGARAVLVGRPVLWGLAAGGREGVGVALGILRR
                     ELDLAMALCGCPNVAAITRDLVAPP"
     gene            7897..8955
                     /locus_tag="VMZ53_27145"
     CDS             7897..8955
                     /locus_tag="VMZ53_27145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32218.1"
                     /translation="MKRAALLVVITACRFNFDEHAGDAGSDTGADAEPARRCTATKVN
                     GFAFGAPGITDLAAAQVPQGYSLGAVHVASTFWGVHLDRQLVPATPGPSWTTPVSPNN
                     GMYENGSLYFDGVNLDGSLTVADGTAYMKTFPTDMTGFFVAVQRTGSAGEPSIARVNG
                     TSLVSTWYHDNVIQFVVLLADGSPDTVDRSFTPGTANIRSVSVADGPSAIIAWSEANG
                     TCGVGSLDAQFGVSLKTLGTACTTPYVHGNGTDVAAVYDWGAYIRTSTIQVQMDGSFV
                     VGNERMIGQGTGPVVIAMGERWAAWRDNSRLHMSPLANIAEAVIENLPSGPPDAYTPA
                     GPYLFAVWGAELWAITCP"
     gene            8986..9642
                     /locus_tag="VMZ53_27150"
     CDS             8986..9642
                     /locus_tag="VMZ53_27150"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS32219.1"
                     /translation="MRIYFVGSHATGKTTLCRYVSRRYGLPMISEVARAVLAEMETGF
                     DALRTDMDLVAEYQERVFARQVAVERMHEGKFVSDRAFDNLAYVAEHTTNAAAMMNDQ
                     RFHDYMKWVREGVVFFLRPHQSLLKDDGVRAGVSWDSVLRIDGMVKLMLEQHQISYLP
                     IESVSMQERVRAVEFVLARCGLMNAPSVTRSRKQTIPPVQKPTVETWPHVLATSDVEA
                     "
     gene            complement(9639..10055)
                     /locus_tag="VMZ53_27155"
     CDS             complement(9639..10055)
                     /locus_tag="VMZ53_27155"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HUS32220.1"
                     /translation="MNRAILPLLSGAIFGAGVCVSGMVRPSKVLGFLDFGGAWDASLL
                     VVMAAALSLHVVAWQIVKRSRAPRYGTAFPDPPSKLIDMRLLGGAAVFGVGWGISGFC
                     PGPAVISVLSGSTASFVFVGAMGIAMVLFDASKSDG"
     gene            complement(10052..10480)
                     /locus_tag="VMZ53_27160"
     CDS             complement(10052..10480)
                     /locus_tag="VMZ53_27160"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HUS32221.1"
                     /translation="MHDFTPVHALVGGALIATSLAIMLIGTGRIAGLSGIFAGFVRGA
                     RGDWAWRAWFLAGMLAVGVAFRIARPETYDYAARVPLWAVALSGVLVGVGTRASNGCT
                     SGHGLCGISRFSKRSIIATMVFFGVGVATATLVGHFVRAA"
     gene            complement(10542..10712)
                     /locus_tag="VMZ53_27165"
     CDS             complement(10542..10712)
                     /locus_tag="VMZ53_27165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32222.1"
                     /translation="MNEDENDDFILLEQHVLDAEDFVEAEVEPVDDYEPAEEVILLDT
                     LRLEDDDLLDFA"
     gene            10933..>15801
                     /locus_tag="VMZ53_27170"
     CDS             10933..>15801
                     /locus_tag="VMZ53_27170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS32223.1"
                     /translation="MSEDSYSTSPSAVVDPLEAKVLVFRQQPEARGFAALRQELRDAG
                     RGELLAELCATWAQHERDPHRAADAWSEAGEAMVVLGETATAIEYLRTALELDPTNDR
                     AADRLLEIVEPNDPAAAAEIIEAELTELAKKNDPKRKPELIARRASHHRRAAVLWNDH
                     LGRVDRALWHYQQAWKLEPHRTESLEAARNLYASLGDDAMVAKLYMAELEVLGKHPPA
                     QKAATIRFALGKLALRGKDLEGAANHLEEAARLDPGNLDISEALADVYANPGFRDGQT
                     RHRAGELFVELGRKRLATRDDATGINYLRRAVGVDPAAKAPSQALESALSETSQWEEL
                     DRILRHRSMVVTEPSERVEVLRRRAALYRNQLKNRDGLIEVLSELIAYEQPGSKVTRE
                     LKELLREDEQWEKLSQLMEAELNALGQDPSTPADLLVSEILELATIAREHLGDRDRAA
                     ELLHQALGVNPTHEEALARYVDHFRERRDWRGLIDLYEFALDNVREAGATADELVRRL
                     EEIAQLAELRLGDIDRAIEAWQRIGELEPGSPKVAEALRRLTARSNMWQQLVASLERE
                     VEMAGDPISRMQALKKMAQTYRERQIDPRRAIDLYEQVLTENPEDDATLKSLVELYEK
                     EGDDAGLANTLKRQLELDLTRLSEQMRRQGKPSEAPKEWPVAKRSERLTTLRRLAQIY
                     ETRLADVDGVVYACRGVLELLSGDRDALDRMERVLEKAHDPRLEQTLEYHAQASASPA
                     ERAKLLRRLAKMATERQDDVVALERWEATLRASPGDPDALAALAGLYERAQRWPELAQ
                     VLERIDGGRPLPPPGTPEAAMRALELERYAMVIDQLKDPVRGVKAWHRVLETTPKNRN
                     ALDALARLYTAGQKWKELAEVVGRQITLMISTGLPDDLARAGELTMERAEILEQKLGA
                     PGDAIRALDTLLSDINPNHLEAHTMLRRLHEARGDFDAAVRVSEREMYLSPDPVRKIS
                     RGLEIGFICRDRLNNPTRALQAFKRVLELDPEQEEALGAAAELLAKLGRWKEHVAMLE
                     RFLQRLPGRDATDDPAIAEQRRALVQRIAAVTADKLGDPQGAFRWWRRAHDEAPDEQT
                     LADVRRAGEAYGRWRELADVITDERKRLVAAGGGAPAEPERFVALSRELAGLFERRLN
                     EKARAIQVYAEALDALPRDLSLLTDLERLASELDQRPVWKSVLEAFDPVIDAATPNER
                     VDLYLRKAKIFEDKMSDAKAAVENVLAAFSWGPDRDEPRVALLALAPKARAWNEVIAM
                     ESALIERGATTEKRVELLRRKAQTIEEQLKDAPRAFRTHLVALLLSPEDADTTSHLWR
                     LARVIGKYRETDKTPKPEPQPAWTQHETALAEAFAAANRAPGARPKSPSHPPPMKNRA
                     HTEPLADGDLSVGDSTQPLDLKEVEVAAQSMALMADSIKRGDKVPAPPNLKQAGDPEF
                     NKENSTMALSHEDIVKLSMPPRPPSPGSGKLPPPPPRPPQITASSPRAKRMSAPPPLP
                     RKAQAPVRRPPLPTLPNRAFETPWEELAVAYESLPAPDAQSRLRWLYRAAEVWETGSK
                     DVARAFDALARAFAQARRTPQGDAEVRARLMRVAQEHKAWDRLADLYEGMAESADTAV
                     AAADLLMEVAQIR"
BASE COUNT         2783 a         5324 c         5189 g         2505 t
ORIGIN      
        1 ggagacgctg cgttcggtgt cgccggctcc tgatggcgag acgtacgccg tggtcctcac
       61 gcccggtccg tacaactcgg cgtacttcga gcacagcttt ctcgcgcgca cgatggggct
      121 cgagctcgtg caggcgcccg acttgttcgt cgatcaggac aaggtcttct gccgcaccac
      181 gcgcggaccc aagcgtgtcg acgtgatcta tcggcgcacc gacgaagcgt ttctcgatcc
      241 cgaagtattt cgggccgaca gcgtgctcgg cgtgcccggc ctcatgcgcg catatgcagc
      301 gggcaacgtc gcgctcgcga acgcgcccgg caacggcgtc gccgacgaca aggccgtcta
      361 cccgttcgtg ccgcagatga tcgagtacta cctcggcgaa cagccgctgc tcgaacaggt
      421 gccgacgtac gtgtgcctgc gcgacgacga tcgcaaatac gtgctcgagc atctcggcga
      481 gatggtcgtg aaggccgtcg atgaagcagg cggctacggc atgctgatgg gcccccagtc
      541 gacggccgaa cagcgcgacg agttccgccg ccgcatcatc gcggagccgc ggcgctacat
      601 cgcgcagcat cgcgtcgagc tgtcgagctg ccccacgtgg gtgggcgatc ggcaccgcgt
      661 cgaaccacgt cgcgtcgatc tgcgcccgta catcctgacg tcgcgcgaag gaccgtgggt
      721 cttgcccggc ggcctcacac gcgtcgcgct cgtcgagggc agctacgtcg tcaactcgtc
      781 gcagggcggc ggcagcaaag acacgtgggt gatgaaggag accacatgat ctcccgcgtc
      841 gctgatcact gcttctggtt cgggcgctac gtcgagcgcg ccgaggccac cgcacgcatg
      901 ctctcggcga ccgcgaacct cgcgctcgat gccgagcttc cgccggcgca ggtgtggcgc
      961 ccactgctcg tcgtgtctgg cgaggaagac gaattccgca cgcgcgtcgc cgaggacaat
     1021 gatgatcact ccgcgtgggg cgacggcgag atcgtccagc gcttcatggt gtgggacgag
     1081 gcgaacggcg tgtcactcac tcggtcgatc ggtggtgcgc gatggaacgc acgctcgatc
     1141 cgcgaggtgc tgtcactcga agcatgggaa gccgtcaacg agctgcactt gcacgcgacg
     1201 agcgacgagg ctcaatcgca gtacacgtat caccgcgagg cgttctatca ccaagtgcgt
     1261 cgctcgacgc agctcgcact cggcctgttg cgctcgacga tgctgcatga cgagccactc
     1321 gacttcatct ggctcggcgt gttgctcgag cgcgtgaacc agacggcgcg catgctcgac
     1381 gttcatcacc attcgttcac gaatctcaac ggcagcaaag agatcgtcga gacggcgctg
     1441 tggttgtcgc tgctgcgcgg cttgtcgggc tccgaggcct atatgaagcg agccgcgggg
     1501 cgcatctcga gtgatgcggt cgcgcgcttt ctgatctcgg aatccgcgtt cccgcgctcg
     1561 atctcgtact gcgtccactc cgcgtacgag cgcctctgtg gcatccgacc gcccgagcaa
     1621 cacgccctgc ccggcgggat ttcgctcgaa cgcctgcgcg tcctcgattc gtgggtcgcg
     1681 gcgcgcagca aggacccgct cggggggcgc agcgtgcacg agctgctcac gcacgtcatt
     1741 gacgaggtcc atgcgatctg cggcacgctc ggcaaagagt tgctcggcgc gtagtgggac
     1801 gccgacgctg gtacgatatc gccgatgcgg gtgctcggaa tcgccgtcgt ggtcgtggcc
     1861 ggatgcggct tcgagcacgg cacactcggc gagagcacca cgctggtcgt cgacgacagc
     1921 gttgaagact tcgccgccgg cgagctgaac gacatcacgg tcgacaagct cggctacctc
     1981 gcacccgacg cgttcgcgct cggtggcctt catgcgcgcg cgtacggcag cccattcctg
     2041 agcgacacct cgacgtggag caacctccag ccgatgctga cgacaccgct cggcgagcgc
     2101 tacagcagca tgcccgggtc gttcgcgttc gatcgcccgt acggcctcgg gctcgcgcgc
     2161 accgacaact tcacggtcgt ctacgacggc gagctccacc tcgatgcagg cacgcgcacg
     2221 ctgcaggtca ccgcggacga catcgcgctc gtcgagctcg atctcggcgg cgtgcgtcct
     2281 gctgtattcg ctcacgtcag cgacggacaa cccgcgcgct tgacgttcga ggtaccgaag
     2341 gccggctggt atccgattcg tgcggcgctg agcgaggcgg gcagcgatgc gttcttgcgg
     2401 ctcgcactcg tcgaggcaaa cacgccgcgg gccgtcgatc ccagcgagct gcgcgcacgc
     2461 gtcacgtcgg cacacggcgt caccgtgacg gcatcacccg atcgagtctt cgccgcgcgc
     2521 gcacccgaca tctcgatcga gccgtcgctc atcgatcgca cgttcgggag cgcagtgccg
     2581 ccgtacgact ttgcgggcgt cagcactgtc tactacgcgc tgcgctacgc cggtcagctg
     2641 cgcgtcgaga cgacggaagc gcgcgtgttc gcgttcgacc tcggcaccga caccacgcgc
     2701 gactacgcgc gcctgctcat cgacggcaaa ccgatcgccg gccgctggcc agggctcatg
     2761 gatcagctga cgagcgaacc gctcgagctc acgcccggct ggcacgacat cctgctcgac
     2821 tacgcgcaaa gcgacgcgac cgcgcggctg caactcacga tgagcagcgc gacgcgagct
     2881 gcgactgcga tcgagccgat ggtcctgcgt cccgtgcgcg atggcggcct tctcacgagc
     2941 gtgtaccgca acgagaacat caatctcgtc gatggtgccg cgctcgatgt cgcgctgaag
     3001 ctcgatgcac ccgcgggggc caccgtgcaa ttcctcgatg cgtacttcac gctgtacagc
     3061 cagacccgca ccgagatgac ggcatcgctc gagggtcctg gcggcgccga tccgatcatg
     3121 attcccacaa cggcgtcgta cgacatgctc tatgactact ttccgaatcg cacggcggcg
     3181 gtgaacgcgc ccgtcgacgg cacgtggcac ctcgtgctca cggataactc gccaacgacg
     3241 actggctaca tctattaccc ggcgctcatg gcgacgtacc gcggcggccc cgataagccg
     3301 tacgccgaga cgatgagcta tgtgtcggca ccgcacgatc tccgcggcac cctgctcgcg
     3361 attcgcgcaa ccggcgagct ggatggcgcg cagctcgtga tcgaagcgcg ctcgggcagc
     3421 gagacgacgc tcgaagccgc gccatggcaa tcacctgagg caacaccgcc cggtgagctg
     3481 gttcagtacc ggctcacgtt gacgagtgat ggttggcagt atccgacgat cgatcgcgtc
     3541 gagctcgaca tccgccgaga cgtgccagct gactgactcg tagtagagtg ccgttgatgc
     3601 cgaacctgct cgcgatgtcg ttcgagggcg agctggcacc atcgttcacg ctgcactgcc
     3661 tcgagcctgg acacacgcta ccggacggct ggggcctcgg ttacttccct gccggcgagc
     3721 cgtcggcagc gatcctcaag gagcccgcac cgccgcaggg ttcgatgcgc agccagctcg
     3781 cgctcgcatg ggagcaagtc gcgtcgtcgc tgttcatgct ccacattcgt cacgcgacat
     3841 ggggcgctct ctccgatgcg aacacgcagc cattcgcgcg cacgtggggc cgccgcgatt
     3901 ggatgatcgc gcatgcaggc agcctcgatc agcgtctgcc agacctcgcg ggtccgttcg
     3961 agcccgtcgg atcgacagac accgaggaaa ttttctgcca gttcctcaac aagtgcgtcg
     4021 agaagaactg gcgcagcttg accgaatgcg acctcgacgt gattaccgga tggctcgccg
     4081 agctcaacgg catcggcggc atgacgctgt gcctcaccga tggccgcgac ttgctcgtgc
     4141 atgccgacca acgcggcgag ccactttgga tcggcgagct gaagccgccc tacgagcgca
     4201 tcgggtttgg cgacgaggat ctcggcgtcg atctgacaaa gcgcggtgcg aaggcgcgca
     4261 agggcgtcgt gatctcgacg gagccgcaga agcctcgcga aggtgatgca gagaaggcgc
     4321 tcacatggcg tcagctcgag ccaggccaag tgctcgtcgt gcgtgaaggc gcgatcatcg
     4381 cggagcgtaa gaccggcggt gccgtcgaga aacctcgcaa cttgcggctg cctgcgaagg
     4441 ccgagccgcg cacgttcgat atcgtgcaca agacgatcta tcgctacgcg caagcgatcg
     4501 agcgctcgca acacgtgctg cgcctgacgc catgccacga tcgattgcag accgtgcgct
     4561 cgcacgagat caacgtttcg gtgccgactg gatcgcgcga gtacgaagac gtgttcggca
     4621 atcgcgtgcg gcggctcacc gtcgagtcgc cgttcaccga gatgatcatc gaggcaaaga
     4681 gcacggtcga agtgctcgat accgatccgc tcgggtttgg cccgctcaag gtcagctcga
     4741 cgattccgct cgtgtggatg ccgtggcagc ggcaagtgct cgatccgtac ttgttgccac
     4801 cggagctgcc cgagacggag ctctccgagc tcgtcgagta cgcgatgagc ttcgtgaagc
     4861 gaaacgagaa cgatctgctc gatacgctcc tcgatatcaa tcagacgatc ttcaaggagt
     4921 acgcgtacac gcaaggcgcg acgaccgtgt cgacaacggc gttcgaggtc tacgtgaatc
     4981 ggcgcggcgt ctgtcaggac ttcacgaatc tgatgatttg cctcgcgcgc ctgctcggcg
     5041 tccctgcacg ctacgtgtgc ggctacatct atacgggccc caaggcggcc aacaccgcgc
     5101 agagtgaggc ttcgcacgcg tgggtgcagc tgtacctgcc acaggtaggc tggaagggct
     5161 ttgacccgac gaatggcatc ctgacgcaga ccgaccacgt tcgcgtcgcc gtcgggcgta
     5221 actacctcga tgcaaccccg acgagcggca cgatcttcgt cggaggcggc ggcgaaacgc
     5281 tgtctgtaga cgtccgcgtc gagcctcacc agggctgata tcatgtgcgc atggttcgtc
     5341 acgtagcgct cgtcgcgctg acggtcgtcg ctgcgtgtgg agacgattcg ggtcaaggcc
     5401 aacccgctga cgcgagcgtc gatgtcgcgg tggatgcagc gccgcgccag atcgtcgcgc
     5461 atgcggtcgt gcgccagatc gtgaacgacg catcgcggag tccggtggcg ggtcccgaag
     5521 tgctctacaa gatgtcgagc gccgcgcagc gcaccggcga gcgcatggat gtgacgttgc
     5581 cgatcggggc ggcaggcgat atcggattcg cgacgctcga cgatcgctac cgcctctccg
     5641 tcgcgctcga tacgacgacg ccgacggaat tccaaatgcg cgccacgcag ctcgagctct
     5701 cgggccgctt cgtcggtcgc tacgaccgca cgccgccggt ggcaggcacg acgctcgccg
     5761 cgacgatcac caacgctcca ggcacgggca ctgcggtgat cacgtcgacc ggcgtgtgga
     5821 cgcaaacagg acgaagcggc ggcacgaacg gctcgttcac gatcgattgg tcgacgcagg
     5881 cggcgagcct cggcggtccg atcggtcttc tcgacgccgc gcagaatgat cgcgcgtact
     5941 acatcgtgct cgacgtgacc ggcaccgcac cgcaatacgc cgcgatctcg tcgtcgtgta
     6001 gcgctgacat cacgatgacc gctggagccc caacggcgtt tacgtgcacc gccgcgcctc
     6061 tcgcgcagtc gtcgtgcatg catgcccagc tacattttgc gggcgagacc gctcgcatca
     6121 ccgcgatcac cggatcgctg tatccgaact acttcaacaa ctggctcctg caggcgacgg
     6181 cgactccgtc gacggggatt acgagcgcgc tgctcctcgc gtatcaagtg ccgctcgcgt
     6241 cgacgacgcc gtcggtcgat ctcgatcgca atttcacgtt cggcaatcca tatcccggca
     6301 ccacgccggt gctgctcatg aacacgggca agtcgcgctc gttcgcgttg ccgggcacga
     6361 acgcgatgaa catctcggcg accgtcaatg tctacctgcc cgcagcgccc gactgcgcga
     6421 cggtcaccga aggccccacc ggcttcgtcg cgatcccgag cctgccgcgg ctcgacggcg
     6481 ttcacctcga tagcgacgca gtcacgatcg gcctcgcctc caagctgcac acgctgacgt
     6541 gggatgtcgc atcgcccggc gcggccgact actggctcgt tcgcgtgcac cgcgcgatca
     6601 tcaacggatc gaattcaacg cgactctccg tcgtgcgctc gtacgtgacg ctcgaccatg
     6661 acgtggtgct cgatccggcg gacttcacgg tcggccagac ctatttcatc gaggtgcagg
     6721 cggtcacggg cgtaccgaaa gcggcgatgg gtgatttccg cgagtacaca ttcccgtacg
     6781 cgacgagctc gatcttctcg tcggcattcc ggatcacgga atgaacgcgc cgctgaacct
     6841 caaggagctc gagacgctcg cacacgctcg gctcgcgcag ctcgcatggg actactactc
     6901 gagcggcgcc gacgacgaga cgtgcgtgaa gcgcaacgtc gaagcgttcc agcagatcct
     6961 gttgcactac cgcgtgctcg tcgacgtgtc gacgcgcgat ctctcgacga cggtgctcgg
     7021 ccatcgcgtc gcgctgccga tcctcgtcgc gccgacggca tttcatcgcc tcgcgcacaa
     7081 agatggggag ctcgcgacgg tgcgtgccgc cggtgatgcc ggcaccatct tcacgctatc
     7141 cacgctctcg aacacggccg tcgaatcggt cgtagccgcc gcgaccggcc cggtgttctt
     7201 ccagctgtac gtctacaagg atcgctcggc caccgaggcg ctcgtgcggc gcgtcgaagc
     7261 tgccggcgcg aaggcgctcg tgctcacggt cgatgctccg ttgctcggcc gccgcgagcg
     7321 cgacgttcgc aacaacttcg cgctgccgtc acagctcggc gtcgagaacc tgcatgcggc
     7381 gggctacgcg ccagtgccac gcgccgatcg cgagagcggg ctcgctgcgt acttcgccga
     7441 cctcctcgat ccgtcgctga cgtgggacgc gatcagctgg ttgcgatcga tcacgaagct
     7501 gccggtcctc gtcaaaggca tcgtgcgcgc cgacgatgct gcttccgcga tcgagcacgg
     7561 cgcggcgggc atcgtcgtgt cgaatcacgg cggccgccag ctcgatgcat cgcccgctac
     7621 gatcgatgtg ctcgctcgct gcgtcgatgc aatcgccggt cgcggcgaag tcctgctcga
     7681 cggcggaatc cgccgcggcg ccgatgtcgt caaagcgatc gcgctcggtg cacgcgcggt
     7741 gctcgtcggt cgccccgtgt tgtggggcct cgccgcaggc ggtcgcgaag gcgtgggcgt
     7801 cgcgctcggg attcttcgcc gcgagctcga tctcgcgatg gcactgtgcg gctgcccgaa
     7861 cgttgcagcg atcacgcggg atttggtcgc gccaccgtga agcgcgcagc tctgctcgtc
     7921 gtcatcacgg cgtgtcgctt caacttcgac gagcacgccg gcgatgcagg cagtgatacc
     7981 ggcgcggatg cggagcccgc gcgtcgctgc accgcgacga aggtcaacgg gtttgcgttc
     8041 ggcgcgccgg ggatcacgga tctcgcagcc gcccaggtgc cgcagggtta ttcgctcggc
     8101 gcagtgcacg tcgcgagcac gttttggggc gtacacctcg atcggcagct cgtgcccgcc
     8161 acccccggcc cgagctggac gacgccggtg tcgccgaaca acggcatgta cgagaacggc
     8221 tcgctgtact tcgacggcgt gaacctcgac ggatctctca cggtcgcaga cggcacggcg
     8281 tacatgaaga cgttcccgac ggacatgacc gggttcttcg tcgcggtgca gcggaccggg
     8341 tctgcgggcg agccgagcat cgcgcgcgtg aacggcacgt cactcgtatc gacctggtat
     8401 cacgacaacg tcattcaatt tgtcgtgctg ctcgcggacg gatctccgga cacggtcgat
     8461 cggagcttca cgcccggcac cgcgaacatc aggagcgtca gcgttgcgga cggaccctcc
     8521 gcgatcatcg cgtggagcga ggcgaatggc acgtgcggtg tcggctcgct cgacgcgcag
     8581 ttcggcgtct cgctgaagac gctcggtacg gcgtgcacta cgccgtacgt ccacgggaac
     8641 gggaccgacg tcgccgcggt gtacgactgg ggcgcgtaca tccggacgtc gacgatccag
     8701 gtgcagatgg acggctcgtt cgtcgtcgga aacgaacgga tgatcggcca aggcacaggt
     8761 ccggtcgtca tcgccatggg cgagcgatgg gccgcgtggc gcgacaactc ccgcctgcac
     8821 atgtcgcccc tcgcgaacat tgcggaggcc gtgatcgaaa atctgccatc cggacctccc
     8881 gacgcgtaca cgcccgcagg cccttatcta ttcgccgtgt ggggcgcgga gctttgggca
     8941 atcacctgtc cgtgatgtca gggtgatctc gtaaatccgt tggcgatgcg gatctacttc
     9001 gtcgggagcc acgccaccgg aaagaccacg ctctgccgct acgtgagccg gcgctacggc
     9061 ctgccgatga tcagcgaagt cgcgcgcgcg gtgctcgcgg agatggagac cggcttcgat
     9121 gcgctgcgaa ccgacatgga tctcgtcgcc gagtatcaag agcgcgtgtt cgcgcgccag
     9181 gtcgccgtcg agcgcatgca cgaaggcaag ttcgtctcgg accgcgcgtt cgataacctc
     9241 gcgtacgtcg ccgagcacac cacgaacgcg gccgcgatga tgaacgacca gcgctttcac
     9301 gactacatga agtgggtgcg cgagggcgtg gtgttcttct tgcgcccgca tcagtcgttg
     9361 ctcaaggacg acggtgttcg cgccggcgtg tcgtgggatt ccgtgctgcg catcgacggc
     9421 atggtcaagc tcatgctcga gcagcatcag attagctacc tgccgatcga gagcgtgtcg
     9481 atgcaagagc gcgtgcgcgc cgtcgagttc gtgctcgcgc gctgcggcct catgaacgcg
     9541 ccgtcggtca cgcggtcgcg caagcagacg attccgcccg tgcagaagcc cacggtcgag
     9601 acctggccgc acgtcctcgc gacgagcgac gtcgaagcct agccgtcgct tttcgaggcg
     9661 tcgaagagca ccatcgcgat tcccatcgcg ccgacgaaga cgaacgacgc ggtggaaccg
     9721 gacagcacgg agatgaccgc ggggccggga cagaagcccg agatacccca cccgacgccg
     9781 aacacggccg cgccgccgag cagccgcatg tcgatgagct tcgacggcgg atccggaaac
     9841 gccgtcccgt agcggggcgc tcgcgaacgc ttgacgatct gccacgcgac aacgtgaagc
     9901 gagagcgcag ccgccatcac gacgaggagg ctcgcgtccc acgcgccgcc gaaatcgagg
     9961 aagccgagaa ccttcgacgg ccgcaccatg ccggacacgc agacgcccgc gccgaagatc
    10021 gcgccgctga gcagcgggag gatcgcgcgg ttcatgccgc cctcacgaag tggccgacga
    10081 gcgtcgcggt cgcgacaccg acgccgaaga acaccatcgt cgcgatgatc gatcgcttcg
    10141 agaatcggct gatgccgcag agcccgtggc cgctcgtgca gccgttgctc gcgcgcgtac
    10201 cgacgccgac gagcacgccg gacagcgcga ccgcccagag cggcacccgc gccgcgtagt
    10261 cgtacgtctc gggacgcgcg atgcgaaagg cgacgccaac agcgagcatt cccgcgagga
    10321 accatgcgcg ccacgcccag tcgcctctcg cgccgcgcac aaagccggca aagattccgg
    10381 acagtcctgc gatgcgcccg gtgccgatca acatgatggc gagcgacgtc gcgatcagcg
    10441 caccgccgac cagcgcgtgc accggcgtga agtcatgcat cgcggaaagc taccccacga
    10501 tcgccgcggt aggcgatgtc gagatccggt cggaggccga gctacgcgaa gtccagcaga
    10561 tcgtcatctt cgagccgcag cgtatcgaga aggatcactt cttccgcggg ctcgtaatcg
    10621 tcgacgggct ctacttcggc ctcgacgaaa tcctcggcat cgagcacgtg ctgctcgagc
    10681 aggatgaagt cgtcgttttc gtcttcgttc atggcttccg tcagtgattg cacccgtcgt
    10741 gccttcggta accctgcgaa tcggcccctg ccccgacgtg tcctcaccga ctcctcgtgc
    10801 ccttggtgtc ccgatgactg ggcgcggtgc gcccggcgca cgatcgaaga atcgtccgaa
    10861 aacctctgct gtgcgctttt gctccggacg cggaaacccg cggagttacc gtggtatcgt
    10921 cgcgagccgg ggatgtccga agacagctac tcgacgagcc cttccgcggt ggtggacccg
    10981 ttggaagcaa aggtcctggt gttccggcaa cagccggaag ccagaggctt tgcggccttg
    11041 cgccaagagc tccgcgacgc cgggcgtggt gagctgctgg ccgagctgtg tgccacctgg
    11101 gctcagcacg agcgcgatcc gcaccgtgcg gccgatgcat ggtccgaagc cggcgaagcg
    11161 atggtcgtgc tcggtgagac ggcgaccgca atcgaatact tgcgcaccgc gctcgagctc
    11221 gatccgacca acgaccgcgc ggccgatcgc ttgctcgaga ttgtcgagcc gaacgatccg
    11281 gcagctgccg cggagatcat cgaagccgag ctgaccgagc tcgcgaagaa gaacgatccg
    11341 aagaggaagc cagagctgat cgcgcggcgg gcgtcgcatc accggcgagc ggcagtgctg
    11401 tggaacgatc atctcggccg cgtcgatcgc gcgctctggc actaccagca ggcctggaag
    11461 ctcgagcccc atcgcaccga gtcgctcgag gccgcgcgca atctctacgc ctcgctcggc
    11521 gacgacgcga tggtcgcgaa gctctacatg gccgagctcg aagtcctcgg caagcatccg
    11581 cccgcgcaga aggcagcgac gatccgcttc gcgctcggca agctcgcgct gcgcggcaag
    11641 gatctcgaag gcgcagcgaa ccatctcgaa gaagcggcgc ggctcgatcc gggcaacctc
    11701 gacatcagcg aagcgctcgc cgacgtctac gcgaaccccg gtttccgtga tggccagacg
    11761 cggcatagag ccggcgagct gttcgtcgag ctcgggcgca agcgcctcgc cacgcgtgat
    11821 gacgcgacgg gcatcaacta tctgcgccgc gcagtcggtg tcgatcctgc cgcgaaggcg
    11881 ccgtcgcaag cgctcgagag cgcgctgtcc gagacgtcgc agtgggaaga gctcgaccgc
    11941 atcctgcgcc atcgctcgat ggtcgtcacg gagccgagcg agcgcgtcga agtgctgcgg
    12001 cggcgtgcgg cgctgtatcg caaccagctc aagaatcgcg atggcctcat cgaggtgctg
    12061 agcgagctga tcgcgtacga gcagccaggc agcaaagtca cgcgcgagct gaaggagctg
    12121 ctgcgcgaag acgagcaatg ggaaaagctg tcgcaactga tggaggccga gctcaacgcg
    12181 ctcggtcagg atccgagcac gcccgccgat ctgctcgtgt cggagatcct cgagctcgcc
    12241 acgatcgcgc gcgagcatct cggcgaccgc gaccgtgcag cggagctgct gcaccaggcg
    12301 ctcggcgtca atccgacgca cgaagaagcg ctcgcgcgtt acgtcgatca cttccgcgag
    12361 cgccgcgact ggcgcgggct catcgacctc tacgagttcg cgctcgacaa cgtgcgtgaa
    12421 gcgggcgcta ccgccgacga gctcgtgcgc cgcctcgaag aaatcgcgca gctcgccgag
    12481 cttcgcctcg gagacatcga ccgcgcgatc gaagcgtggc agcggatcgg tgagctcgag
    12541 ccgggctcgc cgaaagtcgc cgaagcactg cgccgcctga ccgcgcgctc gaacatgtgg
    12601 cagcagctcg tcgcgagcct cgagcgcgaa gtcgagatgg caggcgaccc catctcgcgc
    12661 atgcaggcgc tcaagaagat ggcgcagaca tatcgcgagc ggcagatcga tccgcgccgc
    12721 gcgatcgacc tctacgagca agtgctcacg gagaatcccg aagacgacgc gaccttgaag
    12781 tcgctcgtcg agctgtacga gaaagagggc gacgacgcgg gcctcgcgaa tacgctgaag
    12841 cgccagctcg agctcgatct cacgcgcctc tcggagcaga tgcggcggca gggtaagccc
    12901 tccgaggcgc cgaaggagtg gccggtcgcc aagcgcagcg aacgcctcac cacgcttcgc
    12961 cggctcgcgc agatctacga aacgcgcctc gccgacgtcg acggtgtcgt ctacgcatgt
    13021 cgaggcgtcc tcgagctgct cagcggcgat cgcgacgcgc tcgatcggat ggagcgcgtg
    13081 ctcgagaagg cgcacgaccc tcgcctcgaa cagacgctcg agtatcacgc gcaagcgtcg
    13141 gcgagcccgg cagagcgcgc gaagctgctg aggcgtctcg cgaagatggc gaccgagcga
    13201 caagacgacg tcgtcgcgct cgagcgatgg gaagccacgc tgcgcgcgtc gcccggcgat
    13261 ccagatgcgc tcgccgcact cgctggtctc tacgagcgtg ctcagcgctg gccggagctc
    13321 gcgcaagtcc tcgagcggat cgatggcggt cgcccgttgc cgccgcctgg aacgccggaa
    13381 gctgcgatgc gcgcgctcga gctcgagcgc tacgcgatgg tgatcgatca gctgaaggat
    13441 ccggtgcgcg gcgtcaaggc gtggcaccgc gtgctggaga cgacgccgaa gaatcgcaac
    13501 gcactcgacg cgctcgcgcg gctctacacg gccgggcaga aatggaaaga gctcgccgag
    13561 gtcgtcgggc gacagatcac gctcatgatc tcgacggggc tgcccgatga tctcgcgcgc
    13621 gccggcgagc tgacgatgga gcgcgccgaa atcctcgagc agaagctcgg tgcgccgggc
    13681 gatgcgatcc gagcgctcga cacgctcctg tcggatatca acccgaatca cctcgaagcg
    13741 cacacgatgc tgcgccgcct ccacgaagcg cgtggcgatt tcgatgccgc ggtgcgcgtg
    13801 tccgagcgcg agatgtacct gtcgccggat cccgtgcgga agatttcgcg cggtctcgag
    13861 atcggtttca tctgtcgcga tcgtctgaat aacccgacgc gcgcactgca ggcgttcaag
    13921 cgcgtgctcg agctggatcc cgagcaagaa gaagcgctcg gcgccgccgc ggagttgctc
    13981 gcgaagctcg ggcgatggaa agagcacgtc gcgatgctcg agcggttcct tcagcggctg
    14041 ccgggtcgcg acgcgacgga tgacccggcg atcgccgagc agcgacgcgc gctcgtccag
    14101 cgcattgccg cggtaaccgc cgacaagctc ggcgatccgc agggcgcctt ccgctggtgg
    14161 cgacgcgctc acgatgaagc gcccgacgag caaacgctcg ccgacgtgcg ccgcgcgggt
    14221 gaagcgtacg ggcggtggcg cgagctcgct gacgtgatta ccgacgagcg caagcgcctc
    14281 gtcgcggctg gcggtggagc acctgccgag cccgagcgct tcgtcgcgct gtcgcgcgag
    14341 ctcgcgggtt tgttcgagcg ccgtctgaac gagaaagcgc gcgcgatcca ggtctacgcc
    14401 gaagcgctcg atgcacttcc gcgcgacctg tcgctgctca ccgatctcga gcggctggca
    14461 agtgagctcg atcagcgccc ggtgtggaag tcggtgctcg aagcattcga tccggtcatc
    14521 gacgccgcga cgccgaacga gcgcgtggat ctgtatctgc gcaaggcaaa gattttcgaa
    14581 gacaagatgt ccgacgcgaa ggccgccgtc gagaacgtgc tcgcggcatt ctcgtgggga
    14641 ccggatcgcg atgagccgcg cgtcgcgctg ctcgcactcg cgccgaaggc ccgcgcttgg
    14701 aacgaagtca tcgcgatgga gagcgcgctc atcgagcgcg gcgcgacgac cgagaagcgc
    14761 gtcgagctgt tgcgccgcaa agcgcagacg atcgaagagc agctcaaaga tgcgccgcgc
    14821 gcgttccgca cgcatctcgt cgcgctgctg ttgtcccccg aggacgccga tacgacgtcg
    14881 catctgtggc gcctcgcgcg cgtgatcggc aagtaccgcg agaccgacaa gacgccgaag
    14941 cccgagccgc aaccggcgtg gacgcagcac gagactgcac tcgccgaagc gttcgccgcc
    15001 gcgaaccgcg cgccaggcgc acggccaaaa tcgccgagcc acccgccgcc gatgaagaat
    15061 cgcgcgcaca ccgaaccgct cgccgacggc gatctgtccg tcggagattc gacgcagccg
    15121 ctcgatctga aggaagtcga ggtcgccgcg cagtcgatgg cgctcatggc cgactcgatc
    15181 aagcgcgggg acaaggtgcc ggcgccgccg aatctcaagc aagccggtga ccccgagttc
    15241 aacaaagaga actcgacgat ggcgctgagc cacgaggaca tcgtcaagct gtcgatgccg
    15301 ccgcgcccgc cgtcgccagg ctccggcaag ctcccaccgc ctccgccgcg cccgccgcag
    15361 atcaccgcaa gctcgccgcg cgcaaagcgc atgagcgcac cgccgccgct tccacgcaaa
    15421 gcgcaagcgc cggttcgccg ccctcctctg ccgacgctgc cgaaccgcgc gttcgagacg
    15481 ccgtgggaag agctcgccgt cgcgtacgag agcttgccgg cgcccgacgc gcaaagccgc
    15541 ctgcgctggc tctatcgcgc tgccgaggtg tgggagaccg gcagcaagga tgtcgcgcgc
    15601 gcgttcgatg cgctcgctcg tgcattcgcg caggctcgcc gcacgccgca aggagacgct
    15661 gaagttcgcg ctcgcctgat gcgcgtcgcg caagagcaca aagcgtggga ccggctcgcc
    15721 gacctctatg aaggaatggc ggagtccgcc gataccgctg tcgctgccgc ggatctcctc
    15781 atggaggtcg cgcagattcg t
//
LOCUS       DATLBZ010000543         8778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3351589, whole genome shotgun sequence.
ACCESSION   DATLBZ010000543 DATLBZ010000000
VERSION     DATLBZ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3351589"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VMZ53_27175"
     CDS             complement(<1..242)
                     /locus_tag="VMZ53_27175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32224.1"
                     /translation="MAGTCPRDSSPLQAVGKRLGCTTCRGVFVTQDDLAELVRGMAPD
                     LHGEIKTPFLDRSSKEPALQCPVCTQPMKAVRLGTMP"
     gene            335..1198
                     /locus_tag="VMZ53_27180"
     CDS             335..1198
                     /locus_tag="VMZ53_27180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32225.1"
                     /translation="MARFVLLLLVAFLLGGCIVPHYVQHRAALVPHAAPVQSTGQPMD
                     RVLGFELGATNLADLVAPTTGSVDPPAGIAVAEHQARATFAVAPTPNLHLSFFHEQAL
                     KEGSHDISPTLPPLEDDAAFGYGMGFDVSVPTGAPGWRIGIATELTIWNCPYVAYTTE
                     TTGGTLLVDSGTDPVGIAAFGLTPSYRQGAWTWFANGTIRNHPTMLEKESSDFVAPDP
                     DVTGGPLNVSVGAGVALRRENVEVALDVHDTLTDDPVRYGPSLGLTFRVFGGKSTHRE
                     PAPTTVVVPVH"
     gene            1219..1611
                     /locus_tag="VMZ53_27185"
     CDS             1219..1611
                     /locus_tag="VMZ53_27185"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HUS32226.1"
                     /translation="MAEQQQSEGVDPYRAGFERIQQIIVTTGNEVAGHDVVAYLGIVR
                     GIVVRATSIGKGIIGGFKALGGGNIKEFIEVCELARHDAYQQMIAHAQEAGAEAIIGM
                     RYDAGEFSSGVTEVLAYGTAVKIMPRIR"
     gene            complement(1608..2159)
                     /locus_tag="VMZ53_27190"
     CDS             complement(1608..2159)
                     /locus_tag="VMZ53_27190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32227.1"
                     /translation="MKLLYVVVLALVLATSTARAERLRWYRGFYTRIGVRDSYGMQEP
                     FHAWAAFAFGIGYRYDRERWGIDASALNLQYDKEEGLHTAVRIVPYASLTRWTKAETW
                     AGAGLSIGWLKGTVDQAIPKRKGDGLQAELIAGGELPREMRVRLFVQSTLTLPLYQLY
                     DTNHSRDSTVYVYAFEIALGVRF"
     gene            complement(2156..4678)
                     /locus_tag="VMZ53_27195"
     CDS             complement(2156..4678)
                     /locus_tag="VMZ53_27195"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HUS32228.1"
                     /translation="MRAGVLLVIAMGCSRGTPVPCNGVLEPGELCDDGNSSDGDGCDC
                     ATRLIPPVSYTPVAGDPVVIVEDGGWCWFQDERVVDVDGKVVVSSISHGGDIQVTSWD
                     PASGQREHSILFTRLERDDHDVASLLPLPDGRLAAYFTRHDGFPSLWTRIAQTPGALG
                     SWTPLKVTQFDYDVTYTNPFLVGDRTLLFMRGIDTNPTLVGSTDFGLSWSEGAQLLDS
                     SERELDGRVFDHRPYVKYVSDSAGTVHLFYTDGHPVEFNRNSVYHLVYRDGALYRSDG
                     ARVAGAGLDDQPVLSPSSGTRVYDGNQLPGGQAWTWDAAIGADGKPVTVFSTFPDPER
                     PYYDHRYRYARWDGSAWQVHTIAYGGTGIYVVEGFYSGGITLDPDDPNIVYFSSNVVP
                     QTGEPTTSGKFEIYRGVTSDNGATWRITPVTSGSHVHNLRPIVPAHHTSPTEIVWLRG
                     SYDTYLDYRVQVVGLVGAGDAMANAPVESDADLVALARFDLAATDQGPATPTAPGFVA
                     AVPEGGYALATDRGISLMVSNITATRQAPDAVDPLYRGLALNSRHGFDPGNKLRVELT
                     GLTPGADYVLRVHGHDTKDAYLRTTLWFRGDAKSLDSDNNAAYVLAHRNVSNTTAGEG
                     YSDLFMHADDQGKLSIVGRGLDYYGADHTAVLSGVEVLQPPVTDLVARFDVDALPGDD
                     TAPNAASISWDELTEFSGEATMAGITVRVTTDRIEALRRREDTTDPMLSDFVFGRDQL
                     TVDVSGLEPGRLYAFTVYSTDTKYNLYDASRWRLDEPGREPRIVHGFHMNLQRTDEGA
                     AFTFYHRATTSSFRLRGEDVVTSLSSNPSVVIFNGMDIRVAP"
     gene            4704..5807
                     /locus_tag="VMZ53_27200"
     CDS             4704..5807
                     /locus_tag="VMZ53_27200"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HUS32229.1"
                     /translation="MGRAAWVALLVIGACADEQPAVRTGSACPAQAIREIDDHWMATH
                     PFLGDNDWRRATYFIGHMVVAKTLDDPAYADYLDRWAESHAYQVRDGAFTRNADNHTA
                     GQVYLALYERDHIPDQLAYIKISIDHVVDSGDRGDWSWVDAQFMASPVYAHLGELTGD
                     PRYHAAMFELFADARDRRHLYDASTGLWFRDENYLYPAAHTSHGRKIYWARGNGWVIA
                     SVVRTLEHLAADSPYRAAYEDMLRAMAAGLVSVQRDDGLWNVNLADADDYPGPESSGT
                     AFFVYGIAWGVNHGVLDRATYAPVVERGWRGLVTLAMRADGELGLVQGVGEEPASSQP
                     VTLASTHDYGVGAFLLAGSEVAALGLDLGCGAR"
     gene            5804..6343
                     /locus_tag="VMZ53_27205"
     CDS             5804..6343
                     /locus_tag="VMZ53_27205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32230.1"
                     /translation="MTKHCLLAILVLVAACKREGDHCEYPTKRCDGTSILECHIANAN
                     DDLLGHKSNYFERSSCPAGTQCVDVDGDVGCVDPKAECDPMSTVPVVVERGATIEIKA
                     CTNMLSSNGYYETATFTTCDPRTFKAKCLDGQSALNCVPGEARKGLHSVVREAVRGKY
                     VETQTFCPVCGVDPTQCAN"
     gene            complement(6340..7182)
                     /locus_tag="VMZ53_27210"
     CDS             complement(6340..7182)
                     /locus_tag="VMZ53_27210"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS32231.1"
                     /translation="MTATTLGGRSLATPFAARVFYATNATPMTHTFGPYTFDFAVDAP
                     VLASDLPLVVVSHGMTGTPWGYRGLASHLAHAGFAVLLLEHPGDSRRDPTKAGTAEIL
                     ATRPRDIRAALDAAFADPHVAPHLRAGSAAIIGHSMGGYTALAVAGGKPLALPNQTPD
                     GIAHPVDVERDPRITRAVLLAPAIPWLMAPGALAEVTIPLLVRVGEKDTDTPPFFVER
                     ILASLPASTPLDFATVPRAGHFAFFYPVPPALALLPPGQDPPGFDRAAYQSQLYAELA
                     AFLR"
     gene            complement(7179..>8778)
                     /locus_tag="VMZ53_27215"
     CDS             complement(7179..>8778)
                     /locus_tag="VMZ53_27215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUS32232.1"
                     /translation="LPASSLSRTPDGFGHFVGAAVLDGVIALLLLLTLVGSLLYLARG
                     RLRAVDSHTWLAIGGVFAGALLVRILGLNAFGQTWDEDVNWGAGRNYITNILSGDLSE
                     GSWIWNFEHPPVMKYLEGIGAQFADGFGPARFLSAIWTSLGCALLVPIGMRLYRLRVG
                     IVAGMIAALLPPLVAHSQIVGHESPTVLWWALAILLALGVHDDPARALTRMCWVGVVV
                     GVAVSSRFVNGLVGPLVLAVVIIMAPAERRLRTAIEGALVMPALALATFYVLWPRIWF
                     HPVAMLHQSMIKLSQPHALEPFLGELTNHPGRHYFLVYLAATLPLGILLGVIAYVARA
                     AREHDRSALIVAAWFLLPLGVMFSPVRQDGVRYVMPCILALAVAAAAGFEWLASLAER
                     GKRHVFLPFSGVLALYLVITLWRIHPYYLDYFGEHVGGPATVERKNIFETAWWGEGVN
                     RAVEYVNAHAQPNAKVFRNCIEPAHLAWFRDDLWTPMTNNPDGADWIIAYAPHKVGCP
                     IPASMHKVFTVDAQGAPLAEVWTK"
BASE COUNT         1590 a         2824 c         2972 g         1392 t
ORIGIN      
        1 ggcatcgtgc cgaggcgcac cgctttcatc ggctgcgtgc acaccgggca ctgcagcgcc
       61 ggctccttgc tcgagcgatc gaggaacggc gtcttgatct caccatggag gtccggcgcc
      121 atgccgcgca cgagctcggc gaggtcgtct tgcgtcacga acacgccgcg gcatgtggtg
      181 caaccgaggc gcttgccaac ggcttgtaac gggctcgagt cacgagggca ggttccggcc
      241 atggcgtgac cttacaccga tgggtgagca cccgtgcctg gagctgccga cggtctgcca
      301 ccacctgcca gccggacgcg tgcgagcgta aagcgtggct aggttcgttc tgctcctgct
      361 cgtcgcgttc ctcctcggag gctgcatcgt tccgcactac gtccagcatc gcgctgcact
      421 cgtgccacat gccgcgccgg tgcagtccac cggacaaccc atggaccgcg tgctcggctt
      481 cgagctcggc gcgaccaacc tcgccgacct cgtcgcaccg acgaccggca gcgtcgatcc
      541 gccggcaggg atcgcggtcg cagagcacca agcgcgcgct acgttcgccg tcgcccctac
      601 cccgaacctt cacctgtcgt tttttcacga gcaggcgctg aaggaaggtt cgcacgacat
      661 ctcgccgacg ctccctccac tcgaggacga cgcggcattc ggatacggca tgggcttcga
      721 cgtttctgtg ccgactggag ctccgggctg gcggatcggc atcgccaccg agctcacgat
      781 atggaactgc ccgtacgtcg cgtacaccac ggagacaacg ggtggaacgc tgctcgtcga
      841 ttcggggacg gatcccgtcg gtatcgcggc gtttggcctg acgccgagct atcgccaggg
      901 cgcgtggaca tggttcgcca acggaacgat tcgcaatcat ccaacgatgc tcgaaaagga
      961 atcgtcggac ttcgtcgcac cggatccgga cgtcaccggc ggcccgctca acgtcagcgt
     1021 cggtgcgggc gtggccttgc gacgggagaa cgtcgaggtc gcgctcgatg tgcacgatac
     1081 cctgacggac gatccggtga ggtacggccc gtcgctcggt ctgacgttcc gagtcttcgg
     1141 aggcaagagc actcatcgcg agccagcgcc gacaaccgtc gtcgtccccg tgcactgaga
     1201 tcgcgatata gctgccgcat ggcggaacag cagcagagtg aaggcgtcga cccgtatcgc
     1261 gcaggattcg agcgcatcca gcaaatcatc gtgacgacgg gcaacgaggt cgcgggccac
     1321 gacgtcgtcg catacctcgg catcgtgcgc ggcatcgtcg tgcgagccac gagcatcggc
     1381 aagggcatca tcggcggctt caaggcgctc ggcggcggca acatcaagga gttcatcgag
     1441 gtctgcgagc tcgctcgcca cgatgcatat cagcagatga tcgcgcacgc gcaagaggcc
     1501 ggcgccgaag ccatcatcgg catgcgctac gacgcgggcg agttcagctc gggtgtcacc
     1561 gaagtgctcg cgtacggcac cgccgtgaag atcatgccgc gcattcgcta gaaccgaacg
     1621 ccgagcgcga tctcgaacgc gtagacgtag accgtcgagt cgcgcgagtg attcgtgtcg
     1681 tagagctgat acagcggcag cgtcagcgtg ctctggacga acagccgcac gcgcatctcg
     1741 cgaggcagct cgccgcccgc gatgagctcc gcctgcaggc cgtcgccttt ccgcttcggg
     1801 atggcttgat cgacggtgcc tttgagccag ccaatcgaga ggccggctcc cgcccacgtc
     1861 tctgccttcg tccaccgcgt cagcgacgcg tacggaacga tgcgaacggc cgtgtgcaga
     1921 ccttcctcct tgtcgtactg gagattcagc gcagaggcgt cgatgcccca ccgctcgcgg
     1981 tcgtagcgat agccgatgcc gaacgcgaat gcggcccatg cgtgaaacgg ctcctgcatg
     2041 ccgtacgagt cacgcacacc gatgcgcgtg tagaagccgc gataccaacg cagccgctcg
     2101 gcgcgagcgg tcgaggtcgc aaggacgagc gcaaggacga cgacgtagag gagcttcacg
     2161 gcgcgactcg aatgtccatg ccgttgaaga tgacgacact ggggttcgac gagaggctcg
     2221 ttacgacgtc ttcaccgcgg agccggaacg agctcgttgt cgcgcgatga tagaacgtga
     2281 acgctgcgcc ctcgtcggtg cgctgcaggt tcatgtgaaa gccgtggacg atccgtggct
     2341 ctcgacccgg ctcgtcgagg cgccagcgcg aggcgtcgta caggttgtac ttcgtatccg
     2401 tcgaatacac cgtgaacgcg tacagacgac cgggctcgag accactcacg tcaacggtca
     2461 gctgatcgcg accgaacacg aaatcggaca gcattggatc ggtcgtgtct tccctccgcc
     2521 ggagcgcctc gatgcggtcg gtcgtgacgc gaacggtgat gcccgccatc gtcgcttcgc
     2581 ccgagaactc ggtcagctcg tcccacgaga tcgaagctgc attcggcgcc gtgtcgtcac
     2641 cgggaagtgc gtcgacgtca aagcgcgcga cgagatccgt caccggcggt tgcagcacct
     2701 cgacgccact caacaccgcg gtgtggtcgg cgccgtagta atcgagcccg cggccgacga
     2761 tcgagagctt gccctggtca tcggcatgca tgaacagatc gctgtagccc tcgccggccg
     2821 tcgtgttgct gacattgcga tgagccagga cgtatgcggc gttgttgtcg ctatcgaggc
     2881 tcttcgcgtc gccgcgaaac cacagcgtcg tcctcagata tgcgtccttc gtatcgtggc
     2941 cgtggacacg gaggacgtag tcagcgcccg gcgtgagccc cgtgagctcg acgcgcagct
     3001 tgttgcccgg gtcgaaaccg tggcgcgagt tgagcgcgag cccgcgatag agagggtcga
     3061 cggcgtcggg cgcctgacgg gtcgctgtga tgttcgagac catgagcgag atgccgcgat
     3121 ccgtcgcgag cgcgtagccg ccttcgggca cggcagcgac gaagcccggt gccgtcggtg
     3181 ttgcggggcc ttggtcggtc gccgcgagat cgaaccgcgc gagcgcgacc agatccgcat
     3241 cgctttcgac cggtgcgttt gccatggcat cgcccgcacc gacgagcccg acgacctgca
     3301 cgcgatagtc gaggtacgtg tcgtagctgc cgcgcagcca gacgatctcg gtcggcgacg
     3361 tgtgatgtgc gggcacgatc ggacgaaggt tgtgaacatg cgatccgctc gtcacgggcg
     3421 tgatgcgcca cgtcgcgccg ttgtcggagg tcacgcctcg atagatctcg aacttgccag
     3481 acgtcgtcgg ctccccggtc tgcggcacga cattgctcga gaagtagacg atgttcggat
     3541 catcgggatc gagcgtgatg ccgcccgagt agaaaccctc gacgacgtag atgccggtcc
     3601 cgccgtacgc gatcgtatgc acctgccacg cgctgccatc ccagcgagcg tagcgatagc
     3661 ggtggtcgta gtacggacgc tctggatcgg ggaacgtcga gaacaccgtc accggcttgc
     3721 catcggcgcc gatcgcagcg tcccacgtcc aggcctggcc gccgggcagc tggttgccgt
     3781 cgtagacgcg tgtgccggag ctcggcgaga gcacaggttg atcgtctaag ccggcgccgg
     3841 cgacgcgtgc accgtcgctg cgatagagag cgccatcgcg atagaccagg tgatagacgc
     3901 tattgcggtt gaactcgacc ggatgaccgt ccgtgtagaa gaggtgcacg gtgccggcgc
     3961 tgtcgctgac gtacttcacg tacggccggt gatcgaacac acggccatcc agctcgcgct
     4021 cgctcgaatc gagcagctgc gcgccctcgc tccacgagag tccgaaatcc gtcgacccga
     4081 cgagcgtcgg gttcgtgtcg atgccgcgca tgaacagcag cgtgcgatcg ccgacgagaa
     4141 atggattcgt atacgtcacg tcgtagtcga actgcgtgac cttcaacggc gtccagcttc
     4201 cgagcgcgcc gggggtctgc gcaatgcgcg tccacagcga cggaaagccg tcgtgccgcg
     4261 tgaagtatgc agcgagccgg ccgtcgggga gcggcagcag gctcgcgaca tcgtgatcgt
     4321 cgcgctcgag acgcgtgaac aggatcgagt gctcgcgctg cccgctcgcc gggtcccacg
     4381 acgtgacttg aatgtcgcca ccgtggctga tgctcgagac gacaaccttg ccgtcgacgt
     4441 cgacgacgcg ctcgtcttga aaccagcacc acccgccgtc ctcgacgatc acgacagggt
     4501 ctccggcgac tggtgtgtag ctgaccggcg ggatcaagcg cgtcgcgcag tcgcagccgt
     4561 cgccatcgga agaattgccg tcgtcgcaga gctcgcccgg ttcgagcacg ccattgcagg
     4621 gcacaggcgt cccacgcgaa cagcccatcg caatcacgag caacaccccc gcgcgcacgt
     4681 gacggcatga taactaaagt cggatgggtc gcgcagcctg ggtggctctg ttggtgatcg
     4741 gcgcgtgcgc tgacgagcaa cccgcggtcc gcaccggctc ggcctgcccc gcccaagcga
     4801 tccgcgagat cgatgaccac tggatggcga cgcacccgtt cctcggcgac aacgactggc
     4861 gccgcgcgac ctacttcatc ggccacatgg tcgtcgcgaa gacgctcgac gatccggcgt
     4921 acgccgatta cctcgatcgc tgggcagaga gccacgcgta tcaagtgcgc gacggagcat
     4981 tcacgcgcaa cgcggacaac cacactgcgg gccaggtcta cctcgcgctc tacgaacgcg
     5041 accacattcc cgatcagctc gcgtacatca agatctcgat cgatcacgtg gtcgacagcg
     5101 gcgaccgcgg cgattggtcg tgggtcgacg cgcagttcat ggcgtcgccc gtctacgcgc
     5161 acctaggcga gctcaccggc gatcctcgct atcacgctgc gatgttcgag ctgtttgccg
     5221 atgcgcgcga tcgccgtcac ctgtacgacg cgtcgacggg gctgtggttt cgcgacgaga
     5281 actatctgta tcccgctgcg cacacgtcgc acggcaggaa gatctactgg gcacgcggaa
     5341 acggctgggt gatcgcgtcg gtggtgcgaa cgctcgagca tctggccgct gatagcccgt
     5401 accgcgcggc gtacgaggac atgcttcgcg cgatggcagc cgggctcgtg tcggtgcagc
     5461 gcgacgacgg cctgtggaac gtcaacctcg ccgacgccga cgactacccg ggccctgaat
     5521 ctagcggcac cgcattcttc gtctacggca tcgcgtgggg cgtcaatcat ggcgtgctcg
     5581 atcgagcgac gtacgcgccg gtcgtcgaga gaggttggcg cgggctcgtc acgctcgcga
     5641 tgcgggccga cggcgagctc ggcctcgtcc aaggtgtagg cgaggagccg gcctcgtcgc
     5701 aaccggtcac gctggcgtcg acgcacgact acggcgtcgg cgcgttcttg ctcgcgggca
     5761 gcgaggtcgc ggcactgggc ctcgatctcg gttgtggtgc acgatgacga agcactgcct
     5821 cctcgcgatc ctcgtgctcg ttgcggcgtg caagcgcgaa ggcgatcact gcgagtatcc
     5881 gacgaagcgc tgcgatggca cgtccatcct cgagtgccat atcgccaatg cgaacgacga
     5941 tctcctcggc cacaagtcga actacttcga gcgttcgtca tgcccggcgg gaacgcagtg
     6001 cgtagatgtc gacggggatg tcggctgtgt cgatccgaaa gccgagtgcg atccgatgtc
     6061 cacggtcccc gtcgtcgtag agcgcggcgc gacgatcgag atcaaagcgt gcacgaacat
     6121 gctcagctcg aacggctatt acgagaccgc gacgttcacg acctgcgatc cgcgcacgtt
     6181 caaggcgaag tgcctcgacg gtcaaagcgc gctcaattgt gtgcccggcg aagcccgcaa
     6241 gggtctccac tcggtcgtgc gcgaagcggt gcgcggcaaa tacgtcgaga cgcagacgtt
     6301 ctgcccggta tgcggcgtcg atccgacgca atgcgcgaac tagcgtagga aggcggcgag
     6361 ctctgcgtag agctgtgact gatacgcggc gcgatcgaag cccggagggt cttggccggg
     6421 aggcaagagc gcgagcgcgg gcggaactgg atagaagaac gcgaagtggc cagcacgagg
     6481 gacggtcgcg aagtcgagcg gcgtcgacgc agggaggctc gcgaggattc tctcgacgaa
     6541 gaacggcggc gtgtcggtgt ctttctcgcc gacgcgtacg agcagcggga tggtgacctc
     6601 ggcaagcgcg cccggtgcca tgagccacgg gatcgcaggc gcgagaagaa ccgcgcgcgt
     6661 gatacgtgga tcgcgctcga cgtcgaccgg gtgtgcgatg ccgtcgggcg tttggtttgg
     6721 cagcgcgagc ggtttgccgc cggcgacggc gagcgcggtg tagccgccca tcgagtgacc
     6781 gatgatcgcg gccgagccgg cgcgtaggtg aggcgcgacg tgtggatcgg cgaatgccgc
     6841 gtcgagagcc gcgcggatgt cgcgggggcg cgtcgcgagg atttcggcgg tgcctgcctt
     6901 cgtcggatcg cggcgtgaat cgccggggtg ctcgaggaga aggacggcga agcccgcgtg
     6961 cgcgagatgc gatgccaggc cgcggtagcc ccacggcgtg ccggtcatgc cgtgcgagac
     7021 gacaacgagc ggtaggtcgc tcgcgagcac gggcgcgtcg accgcgaagt cgaacgtgta
     7081 tgggccgaac gtgtgcgtca tcggagtcgc attcgtcgcg tagaacacgc gggcggcgaa
     7141 gggcgtcgcg agcgagcggc caccgagcgt cgtggccgtc atttcgtcca gacctcggcg
     7201 aggggcgcgc cttgcgcatc gacggtgaag accttgtgca tcgacgccgg aatcgggcag
     7261 cccaccttgt gcggcgcgta cgcgatgatc cagtccgcgc cgtcggggtt gttcgtcatc
     7321 ggggtccaga ggtcatcgcg gaaccacgcg aggtgcgcgg gctcgataca gttgcggaag
     7381 actttcgcgt tgggttgcgc gtgcgcgttc acgtactcga cggcgcggtt cacaccctcg
     7441 ccccaccacg ccgtctcgaa gatgttcttg cgctcgaccg tcgcgggacc tccgacgtgc
     7501 tcgccaaagt agtcgaggta gtacgggtga attcgccaga gcgtgatcac gaggtacagc
     7561 gcgagcacgc cgctgaatgg aaggaagaca tgacgcttgc cccgttcagc gagcgacgcg
     7621 agccactcga agcccgccgc cgcggccacc gcgagcgcga ggatgcacgg catcacgtag
     7681 cggacgccgt cttgccgcac gggagagaac atcacgccga gcggcagaag aaaccacgcg
     7741 gcaacgatca gggccgagcg atcgtgttcg cgagcagcgc gcgcgacgta ggcgatcacg
     7801 ccgagcaaga tgccgagcgg cagcgtcgcg gcgagataga cgaggaagta gtggcggccg
     7861 ggatggttcg tgagctcgcc gaggaatggc tccagtgcgt ggggctgcga gagcttgatc
     7921 atcgactgat gcagcatcgc gaccgggtga aaccagatgc gcggccagag cacgtagaac
     7981 gtggcgagtg cgagcgccgg catcacgagc gcgccttcga tcgcggtacg cagacgccgc
     8041 tcggcgggcg ccatgatgat cacgaccgcg agcaccagcg ggccgacgag gccgttgacg
     8101 aagcgcgagc tgaccgcaac accgacgacg acgccgaccc agcacatgcg cgtcaacgcg
     8161 cgcgccggat catcgtgcac accgagtgcg agcaaaatag cgagcgccca ccacagcacc
     8221 gtcggagatt cgtgcccgac gatctgcgag tgcgcgacca gcggcggcag cagcgcagcg
     8281 atcatcccgg cgacgatgcc gacgcgcagc cgatagagcc gcatcccgat cggcacgagc
     8341 agcgcgcacc ccagcgacgt ccagatcgcc gacaggaacc gcgccggacc aaagccgtcg
     8401 gcgaattgcg cgccgatacc ttcgagatac ttcatgactg gcgggtgctc gaagttccag
     8461 atccacgagc cctcgctgag gtcgccggag aggatgttcg tgatgtagtt acgccccgcg
     8521 ccccagttga cgtcttcgtc ccacgtctga ccgaaggcgt tgagtccgag gatgcggacg
     8581 agcagcgcgc cggcgaagac gccgccgatc gcgagccacg tgtgcgagtc aacggcccgc
     8641 aaccgcccgc gcgcgagata gagcagcgag ccgacgagcg tgagcagcaa gagcagtgcg
     8701 atcactccgt cgagcacggc cgcgccgacg aagtgaccaa acccgtccgg ggtgcgggac
     8761 aacgagctcg ccggcaaa
//
LOCUS       DATLBZ010000544        28204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5919528, whole genome shotgun sequence.
ACCESSION   DATLBZ010000544 DATLBZ010000000
VERSION     DATLBZ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 28204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28204
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5919528"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VMZ53_27220"
     CDS             <1..1290
                     /locus_tag="VMZ53_27220"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HUS32233.1"
                     /translation="ADKKMTAKLTLPDGKESTLPIIVGSEDEHAIDIRSLRNETGYIT
                     LDSGYMNTGSTTSAITYLDGEQGILRYRGYSIEDLAEKSNFVEVSYLLIYGKLPNRGE
                     REVFGTMLTRHSLIHEDMHHFFAGYPPTSHPMAVLSSMVTSMSAYYPDSLDRNSAIDL
                     HITRILSKVRTIAAFAYKKSIGQPLMYPKNSLRYCANFLHMMFAVPSEEYEVSPILEK
                     AVNHLLILHADHEQNCSTSTVRMVGSSGASIYAAIAAGILALWGPLHGGANQAVIEML
                     EEIRASGSDYKKFIDDVKNKRDNRRLMGFGHRVYKNFDPRAKILKKMCDEVLGHMGIK
                     DPLLDIAKNMEEIALKDDYFISKKLYPNVDFYSGIIYRALGIPTNMFTVMFALGRLPG
                     WIAHWKEMVEDPQTKIGRPRQVYTGSKLRKFVALEQR"
     gene            1297..1869
                     /locus_tag="VMZ53_27225"
     CDS             1297..1869
                     /locus_tag="VMZ53_27225"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HUS32234.1"
                     /translation="MGKGRLEAFSDGVIAIIITIMVLELKVPHGTSFEALRHVWPPLA
                     AYVVSFIYVGIYWNNHHHLVHTVKHVDGWVMWANMHLLFWLSLIPKATEWVGEDLHEH
                     VPLAVYAFVMLMAGVSYLLLTKAILRQGDNPQLKEALGKDYKGNLSGVLYAAGVGLAF
                     VSRWITIAIIIAVAILWLIPDRRLVKAAAK"
     gene            complement(1870..2553)
                     /locus_tag="VMZ53_27230"
     CDS             complement(1870..2553)
                     /locus_tag="VMZ53_27230"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HUS32235.1"
                     /translation="MKNFCGTGISKAPCHARRMRWLLMLLASCGQMYSKPASQESTAA
                     PDDVEAPAPAKPLPTSKPVEKAAEKPATKAEAAKGPSDPAAKAFVDAHNAARAKNCAP
                     PLTWSPQIAKVAQAWADQLKAKGCVFGHSGNMKYGENLAAGTIGALDPESTVAMWYDE
                     IKLYKFPDGGFSMQTGHFTQLVWTTTKQVGCGHVQCKGNDIYVCNYDPPGNYEGEYKT
                     HVLPTSCKK"
     gene            complement(2550..3356)
                     /locus_tag="VMZ53_27235"
     CDS             complement(2550..3356)
                     /locus_tag="VMZ53_27235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32236.1"
                     /translation="MMRALLFLLLAACQFEHGSLSTGDGGGGGGDGNDAMRDSGPPCD
                     VHDFDGDGLGDSCDLCPHIASANIDQDDDGVGDECDERPLSPGEHRALWMAFYDPSEI
                     AGWTEPQNSVGVWNVSNNLLHETMNGFSVLDSPLTYTTDAYFYTSIQITQPNTNEVGF
                     CLSDVQPSVQYYCCAASNAAGPSVRAASQYNGSPGQISSPTGFAGNLGVGQHIEMKGT
                     LIGSQFRCRFTQGTAVSNAMTAAVGRAGPACFYTTTPVDYRYVFLVTIGS"
     gene            complement(3353..3793)
                     /locus_tag="VMZ53_27240"
     CDS             complement(3353..3793)
                     /locus_tag="VMZ53_27240"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HUS32237.1"
                     /translation="MAKKKSLPTDKTVKELRDWGLATYPGTALKSPWPGHMDLAVNDK
                     TYAYLSVEGEPFSISCKLPQSSSVALMLPFCKPTAYGLGKSGWVSAQFPEGRPLPVEM
                     LKEWIDESYRAQAPKKFLKQLEGGAPAAPAKKPTKKKPAAKKKK"
     gene            3816..4241
                     /gene="apaG"
                     /locus_tag="VMZ53_27245"
     CDS             3816..4241
                     /gene="apaG"
                     /locus_tag="VMZ53_27245"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HUS32238.1"
                     /translation="MPRPTVAESTSSAVTQGIRVRVQSHYLPEQSKPNEDHYVFAYTI
                     TIANESTYTAQLRTRHWIITDGRGEVEEVKGDGVVGEQPRLSPGQSFQYTSGCVLTTP
                     IGTMQGTYRFWRDDGTYFDAQIAAFSLSMPLQERNEEAN"
     gene            4326..4988
                     /locus_tag="VMZ53_27250"
     CDS             4326..4988
                     /locus_tag="VMZ53_27250"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS32239.1"
                     /translation="MTNAEREAATATALVEAAKGGDDKAFEALVRRYRKRIYALALHM
                     TRSASEADDITQEVFLKAYRALPGFEGRSQFFTWVYRMTVNRSLNAKRDRARRGEDTI
                     DDPRLELAVAVDARGNPAREAELRQTYSRLLRALDQLPIEMRTTVILVSLQGMSHGEV
                     GVIQKVSEGTIAWRMHEARRRLHEAMVPEKVRRPRGELSSELSRVLTEYGLPLIPVKG
                     RA"
     gene            5014..5889
                     /locus_tag="VMZ53_27255"
     CDS             5014..5889
                     /locus_tag="VMZ53_27255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32240.1"
                     /translation="MRFAIALLIVAACGDEGPVADPYQCVASGGEACFELPSYRIRAA
                     DPMGVLAEPALDCAPFSPSSLGDATFSGHTADYASIVGDVRVEVFSDVAMTDSLAAVQ
                     SDADGVFSADIAAMPNQMFVRTTADGRLPTLRLYQRFDVAPTGYYATTLADADVTSML
                     AGPGDRRLPGKSQLRGIVNDCAGKRLINVVANIAPSSGKNGWRLFEPGVRTYYQSDFD
                     GSFLRRTVGSQTTPAGIFLATNLAPGHHFVQVWGFLTDEDLMLGSLGLKLLAEDEIVV
                     PDADSVVVTHEYARL"
     gene            complement(5886..6545)
                     /locus_tag="VMZ53_27260"
     CDS             complement(5886..6545)
                     /locus_tag="VMZ53_27260"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HUS32241.1"
                     /translation="MLTVHHLENSRSQRVLWLLEELAVPYEIVKYARDPKTSLGPPEL
                     QKIHPLGKSPVITDDGTTVAESAAILEYVAEKYGNGKLIPAKSSPEYQRYRYFMHYAE
                     GSVMPILLVKLITRRLREAPGVAKLVGKAVAGKLDAAFVKPNVDRHVPFIANELGSRP
                     YFCGAELTLADIQMSFPMEAIAARIPDAPKVIHDYVARIHGRPAFKAALDKGGPYDVM
                     D"
     gene            6592..7110
                     /locus_tag="VMZ53_27265"
     CDS             6592..7110
                     /locus_tag="VMZ53_27265"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS32242.1"
                     /translation="MRFLLAGVLVVAGCSQLMPAPKQEPFPLLEVKATRPPPGPARVV
                     LTPSNVAIMDKVQFETGKADIKPESHGLLDEVAKVLKENPQVEQISVEGHTDSTGSPE
                     FNRKLSQQRAESVAKYLSGKGVKAARMEPKGFGPDRPIADNESDAGREANRRVEFNIL
                     KQGPKKTLVKDE"
     gene            7103..7474
                     /locus_tag="VMZ53_27270"
     CDS             7103..7474
                     /locus_tag="VMZ53_27270"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS32243.1"
                     /translation="MNKATLIIAALTLASVAACAKKGMSPAVRSEIETKLAATQKPIE
                     ECYQRQLTVNRKLQGMVVATAAIQPDGTFSEVTLRRDEPQDPVLKFCVVQEIAKIKLD
                     KPMGERVVLDSIPIKFVWANP"
     gene            7474..7905
                     /locus_tag="VMZ53_27275"
     CDS             7474..7905
                     /locus_tag="VMZ53_27275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HUS32244.1"
                     /translation="MAKLPTRSPTHEEVDPTRLASIGLELEEIADGEEIPLPALVELA
                     EDRGRPASHYLAAIPLATELRLAGKAPISVRVCAGNCQRYGALDLLDALVEKPGIQIV
                     PVTCLDRCDQAPACEVHSASGQLVVAPASKSNLDEALAALA"
     gene            7993..8706
                     /locus_tag="VMZ53_27280"
     CDS             7993..8706
                     /locus_tag="VMZ53_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS32245.1"
                     /translation="MLRLHKVNKYYQVGTHSLHVLRDIELDIADGEFVAIMGASGSGK
                     STLLNILGILDDYDSGEYWLGDTLIRNLNESKSAAYRNRFIGFVFQSFNLLPFKTAIE
                     NVALPLYYQGVSRRKRNQIAMQYLERVGLRDWADHLPSEMSGGQKQRVAIARALIGKP
                     RLILADEPTGALDSETSRQIMDLMHEINQTGITVLIVTHDPEVAARCQRTIRLRDGKV
                     MGPGDAASVSNPGMPAVTG"
     gene            8706..10079
                     /locus_tag="VMZ53_27285"
     CDS             8706..10079
                     /locus_tag="VMZ53_27285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS32246.1"
                     /translation="MFVAILKLVGLLVGLAVVLALLATLVVIAIALVRMLFVTLVQWR
                     DLFSIDRWQEVLVTIGRNKLRTALTMISVAWGIMVLVSLLGLGVGLDQGMRKQFARDA
                     TNGVWISANKTSVAYGGYDVGRKVMFENGDYERAKKIEGIEYITAQHYIGGRRWGANL
                     VTKRGAKANTFELQAVHAPVFHLEANEMIVGRFLSDADIAQRRKAAVIGKNVADFLFG
                     GEPAVGDWITIGGVAFQVVGVFTNPGGAEEERKIFIPITTAQLSYNGGEKVGSIQFTV
                     GNAGPEETQHIIDQVVGQLADRWSFDPNDKQAVRVHNNIEQFERFKKLFWMISMFVIV
                     IGLGTLAAGVVGVSNIMMIAVKERTKEIGVRKALGATPSSIVFMVIQEAVFLTALAGL
                     FGLVGGLVILEGLGKMNNDFILNPSIDLRTGIGATIFLVIAGALAGYFPARAAARVNP
                     IHALRDE"
     gene            10095..11348
                     /locus_tag="VMZ53_27290"
     CDS             10095..11348
                     /locus_tag="VMZ53_27290"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS32247.1"
                     /translation="MFDLDKWKEIWATLRSNRLRTFLTAFGVFWGILMLMAMLGFGSS
                     IQTGSQKQMRGMATNLLFVWGQQTTESYDGLPPGRQVKFDIDDIELLRRLPGVEHLAP
                     RLQLGGWMNNFTVQHDTKSGTFTVMADYPELKYIIAFEYEAGRFINERDIAESRKVVV
                     IGKAIQEQLFAPGEDPIGQYLKISGVYFQVVGVTKTLRTGQQGERDANSAFVPFTTLK
                     TAFNMGNRVGFFAMTAKPGTDGPELERQVRGALAQHHRIAPNDQLAIGSFNMFVMFGK
                     FTTFFFVLKALSWIVGGLTLLAGVVGVSNIMLITVKERTKEIGVRKALGATPMSVISM
                     VMKESIVLTLIAGLIGVSVGVALLAGAEMALKNATDSPLGPPHVDLSTVLVAVGVLVV
                     AGALAGIMPASHAASIKPIEALRAE"
     gene            11351..12505
                     /locus_tag="VMZ53_27295"
     CDS             11351..12505
                     /locus_tag="VMZ53_27295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUS32248.1"
                     /translation="MKKILTWLIAAVVILFIGGAFVFLYKKSQAKPVTFQTETADYAD
                     ITKKTVATGSIVPRREVEVKPKVTGVLSELYVEPGKKVKTGDALGKISIIPDAMQTQQ
                     AQTGVKAAQIAFDNAKRELQRNEELYKKGVVADAELQRYRTEFALRQQELEAAGGRLQ
                     LVKEGATRGQGSQSTLIVTSTADGMVIDVPVKVGFSVIQSNNFNPGTTVATVADMDDM
                     IFDGRVDEAEVAKIKEGMKLSIKVGAIEKDTLEGVLEYISPQGKTIDGAIQFQIKAAV
                     KQKPDVMMRANYSANADIVLDEKKHVLAVREALVQYDNGKPYVEVETSPQTFVKKDVQ
                     LGLSDGIKVEVLSGVAATDKIKIPETAGPPSAMGSGGPGGKGGGKPPAKK"
     gene            12593..14158
                     /locus_tag="VMZ53_27300"
     CDS             12593..14158
                     /locus_tag="VMZ53_27300"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HUS32249.1"
                     /translation="MLGAIDAGSNAIRVVIAELGVTELVRIEAERIPVRLGRNAFTRG
                     ELDEKTIDDAVGAFVHFRQRFDAHGVVLYRAVATSAVRNASNRDVLLHRLYHEAGIEL
                     EVIEGEEEARLVRKAVMQSLGPQAVPRAILDLGGGSLEVNLKAGSVWRGHSLPVGTVR
                     LLETFGLDGAIADAEAGMVRRYTATLMHTIAKNGPQSGTAAITGGNAEALARIIAKDG
                     SNSFELADLEKALPDIVSATVEQRMDKLGVKRDRAEVLGIASLVFATAARQLGLQKLV
                     APGVGVREGVLIELGETAREEQAKAEGAHDKALLTAARSFANRVDHDTTHGEHVRLLS
                     RALFHQLRDVHGIPDDKGVLLEVAALLHDVGEVVNQRGHHKHSEYMIRWGRIPGLDDA
                     GREMVALMARCHRKDIGRAKEMINASQLPKEQRVQLRRMTALLRIADSLDTDHRSRVE
                     QVVCTRMGEAIVLDLVVRDGPTREDPRLLRKTDMLKDELGLDVRVTVARPVAVPVEAG
                     TSGELRAVRSRML"
     gene            14502..16112
                     /locus_tag="VMZ53_27305"
     CDS             14502..16112
                     /locus_tag="VMZ53_27305"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HUS32250.1"
                     /translation="MRGALLVCLTLAACGDDPPTGPLAYHITHYDYTFDVESRAAHAE
                     VTLAVDTGGDCFDIPFRADGAANATIDGDAVSAAPDGTKLMVCGTGYETGSTIKLGID
                     VQIKLATVGPSQVGYSVTQDTDGNKLYYLVSWVGGCDQFGPCDSRPDQFATYKFTVTH
                     PASFKTRCPGTITEPSATQTVCDFTLAGGPTYSTFGVATYPAWTESDKGTWGGIKVTL
                     YDRASTTINAAIDSAWHDGYMTWMQATFGPYPFGNELRVLTAPTYWSGFEHPGNIVLD
                     DSLAKLTRPRYWSETAHVLDHEIAHQWAGDQTTLKDTYDFVWKESMAEYLAYVYEDMQ
                     DPAKSLVTARYWKTAAAAAEYFPVPGEKPALFDYYGDVYGEGPMILFHQLETMYGRDK
                     VIAALQKVLGKEQAIGVDDVIAALQETTGTDLSAYAAAWIHGSGAPDWPKYNVTFTPG
                     SLSLTMMNKKSTPRGCMFHVALEGANTGESLVMTVDTRTAQDQTIPVAPLSFTVTKVT
                     LDPYAECLVYKASGTPRMIRENPWVSEH"
     gene            16243..17265
                     /locus_tag="VMZ53_27310"
     CDS             16243..17265
                     /locus_tag="VMZ53_27310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32251.1"
                     /translation="MLMLASCGGKLPGGKGVPGGDKIPGGGNVPGGLGGDSGMVDPNT
                     CGNYAGMEAGARLKAFLSAVADLDKQAKETVEVVRTSCKMMGKELGMSDADFTDGMET
                     KDVCAKVWGAYNDNMKVAVKSKAAFKVKYTPAVCKLSVNAVAEAAAKCEGKASADMGA
                     TCSGTCNGKCSGKCAGSGKAGTGGSSGSGECHGQCSGTCEGDCEGHADVKASGQCKAH
                     AQVEASAKMECTEAKLEVTLDAKLVVDKSKAEQTLNAMKNGLPKLLSLNARLKPLQAA
                     VETTVSTAKELKNMGPKFVNSFKDQALCISGQVAAALNAAMSIQANVSVSVSVSAEAS
                     GSVGGG"
     gene            17382..18845
                     /locus_tag="VMZ53_27315"
     CDS             17382..18845
                     /locus_tag="VMZ53_27315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32252.1"
                     /translation="MKTLTEFSPLLLRNAAAARKAAGTGSPAPAASEEAPAAEAPPAA
                     EEAAAEAAPAEGDASAEASADASAEASADASSEASEGEAVEASGEEPEPMKEEGEPKA
                     DGGAAKAPDPAIEPVAAALGVQADRAARLLEALDIIGDRIDQVRLVRVFQGEKGPHGA
                     ITRGEFHYVIDRVAGAQRARRDDRGGDRGGRGGDRGGRGGGGDRGGRGGGGPGGGGGG
                     GGFGGRGDRDKPRGLGSLKMGAPKEEKKDDDRPARGEMPRAGIGWQLTAAPRDFSDRG
                     GRGGPGRGRGDRDRRGPGGGRGPGGPGGGRGPGGPGGGRGPGGGRGRGPGGPGGGRDF
                     GPGGGRDFGPGGGQPGVEGGAPGGEGFAGGGGGGGEFRGRGPRRGGGGGGGGPRGPRP
                     EGQNASGQNAGGDRPPQQPRLGPDGQPLPPRSKRPRKPIGPDANGMGPDGKPWDPERR
                     AKRQAERETPVNNEQGSSVEGQTPSAPVQNQSETPTE"
     gene            18940..19395
                     /locus_tag="VMZ53_27320"
     CDS             18940..19395
                     /locus_tag="VMZ53_27320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32253.1"
                     /translation="MSAAVIAVAVAATTQPVLADMSRPVISAFKGQLVVSKDELPTGK
                     NDKDTIAKIKKEQLKELTGTPGEDNTSWNFHYTAFLSKTGAKNLKMEFMKGDKLAADK
                     QLDGIDPKSTVLSGDISIDENEGLAKGNTYTIKLLAGSAVVAKTTLIMK"
     gene            complement(19396..20604)
                     /locus_tag="VMZ53_27325"
     CDS             complement(19396..20604)
                     /locus_tag="VMZ53_27325"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS32254.1"
                     /translation="MRIATIIAGAVTASLVPSLAAAEAVRDLPDPSAITFVRKEIPPS
                     GVYALAQSKIIYLNHTGVTLRPGNDDARTNRSSIVSQQVSVPAWNTSAAHWQQTVTCM
                     KDMFKRWDVVVTDVDPGNVPHMEAVFGGSPQNVGMGQGVGGVSPFTQDCGVIENSIVF
                     TFTSVFPDDPQTVCEVMAQEVAHSYGLDHEMLASDPMTYLNYNGNRTFKDQTVACGEY
                     SNRPCGIGGSTCRPNQNSVQLLNQRLGQADLVAPTLGITSPADGATVEPGFAVDATAS
                     DNIAVTAAQLFIDDQPVTTAAGAGPYTFATDAALAEGVHTISVEATDGKNPTRKSITV
                     TVAKAGSGDGSGSGSGSDEDGDGVPDGAGGDGDDDGDLIGGCNSGHDVGFAVGLFVLA
                     QIVLRRRRYD"
     gene            complement(20803..21738)
                     /locus_tag="VMZ53_27330"
     CDS             complement(20803..21738)
                     /locus_tag="VMZ53_27330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32255.1"
                     /translation="MRCRWAVVLLLALAACRHSSSSGSGSEPAHWAKAGPAPVAATLT
                     YPLELSIGGGRVWVVASNRDVMLLDRGKPRIVKSLGWVSAIAADEEGAWIAAHDELGG
                     KTSALWRLLPDADPIRVAEDVTYVAPAGAAIVIWRTGGAFVREPSGAEHPLGAIEGRI
                     RAVGDTLYVGDRRGVISRMPLAGGPLEQVADFGALVVSFDVAGDTIYASTDRGIYTMS
                     TRGGPRQMLAEHLDDPREVVADGEGGVYVLTYGTTSGLWGHSTNDDGELLHIDRNRKI
                     TALASDIIGAGRIALTKDTVYATDVYGGRLLAVPR"
     gene            complement(21768..22121)
                     /locus_tag="VMZ53_27335"
     CDS             complement(21768..22121)
                     /locus_tag="VMZ53_27335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32256.1"
                     /translation="MHRLLNRYRLQAKDFRPKGGGSIIARAALKNLAASSPAPVRTFG
                     GRDFSRTAIREVEARRPEYVAPALPAPADRFDQSDVVARPSSERTYAPHHRPAYQSDL
                     GHAALHALAQRKIAG"
     gene            22561..22857
                     /locus_tag="VMZ53_27340"
     CDS             22561..22857
                     /locus_tag="VMZ53_27340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32257.1"
                     /translation="MKSGFETIESNELVTVAGGAGGTSVPTSSGTNDQLLSTVQGIQS
                     SLKDLSSNQNNQGLFNGPNGALAFGMMAMAMRRPASTTVVYGGRRGGYYWQSSW"
     gene            22851..23147
                     /locus_tag="VMZ53_27345"
     CDS             22851..23147
                     /locus_tag="VMZ53_27345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32258.1"
                     /translation="MVRTNMNHFQTIATTSLATVTGGKATSAQDTQLTAMLTDISKSI
                     KDAAEAKNKSSSDPTQMMMMMMMMGGGGGGSQQAVAAAPPPPAPSPNVVRVNVR"
     gene            complement(23151..25118)
                     /gene="tkt"
                     /locus_tag="VMZ53_27350"
     CDS             complement(23151..25118)
                     /gene="tkt"
                     /locus_tag="VMZ53_27350"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HUS32259.1"
                     /translation="MTTDLPTLSINTIRTLAMDAIQKANSGHPGLPMGCAPMAYTLWQ
                     RHLKHDPKSPLWFDRDRFVLSAGHGSMLLYSLLHLAGYALPLDELKNFRQWDSKTPGH
                     PEWHLTPGVEATTGPLGQGAANAVGMAIAERYLGRYFNRPGHTIIDHHTFALVSDGDV
                     MEGVACEAASLAGHLGLGKLTYLYDANQITLDGPLSLTMSEDVGARYAACGWHVQHVE
                     NGDTDLAALDAAIAAAKAETDRPSIIVIRTTIGYGSPKKAGTSSAHGSPLGDAEIAAT
                     KQALGWDPNAKFLVPDEVRQHMSEGGRRGAAAHAAWREKMTAYKAAHPDLAAQFDMAV
                     NGELPPGWDAEFPSFTGSMATRACSGKVMAAITAKIPWLFGGDADLGGSTKTIVPGGD
                     YVKAGEGRNLRFGIREHAMGAIGNGMLYHGGARSYVSTFFVFSDYMRPTVRLAALNNQ
                     PAIFVWTHDSVGLGEDGPTHQPVEHLMALRAMHNLTLFRPCDGNETAVGWKLALLRNG
                     PTGLVLSRQDLPTLTQKGAPGAERGAYVLADGTDVILMASGSEVAVAMGARELLAKEN
                     ISARVVSMPSWEVFEEQDPAYKAQVLPPDRWQRVSVEAGVTFGWRQYIGERGIAIGID
                     RYGASAPAEVIFEHLGITPANVAAAARKLVR"
     gene            25158..26987
                     /locus_tag="VMZ53_27355"
     CDS             25158..26987
                     /locus_tag="VMZ53_27355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32260.1"
                     /translation="MRRLKALILVGIWACGGGKPATQPVPITPPAPTPVPTASPTPTP
                     TPTPTPAPTAEKPFTVDDKSGPEFFPPARKKVAIGQTISFSTAIIDQDLDETRVEVTR
                     MPKTATYDAITQTVTWTPGADEAYTLPKGSGLKEKALPQFELSVTQPGREKTAKYEVL
                     FEVVKGKPIAEPVAPQQTAVIETLLMIRSPKRLEQVNKDWPLDKMLAVGAETFKLQIP
                     EDKRSKLKGSLDGNTSYTQMLAGLAQTHNNPRLDPTSPQFDKAAFGSPADWKIVTFRP
                     RIDRAWTELRVVYRAVKAQEPVFAMFRLRPVVEYVPALPRPEEEHEANNKIFLGMVAK
                     HLMKNGGPNPKFVKDEAAEGKAVSALLTELMSFDDTKTKPYLHGFEIGIALEARMGGG
                     SARNADGSYKSGDGWGWSAQKPFQTSEGSTQVYQNVAIPGFWTQTVASKDNTTWVPRC
                     APRFNPEDKFVPGYEILCRKTMGFVDLPATKDGKIVNGRVDANNMYFEHKKRWMVEGF
                     PLEDGRRDLGEENGMTCSQCHIRNFGMHDYSDPANVDPSKGTPKALNKSLATLNFQIV
                     PSATWEEFTIDFLHHQECRGKTNLEKYLGADSAKGLTCPLAPK"
     gene            26997..>28204
                     /locus_tag="VMZ53_27360"
     CDS             26997..>28204
                     /locus_tag="VMZ53_27360"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HUS32261.1"
                     /translation="MAAPAAPKKKLPLAPADDKPAKKPEGKAAKIRAGGWHAFTDAGT
                     APKSLTAARIVMTLALAATIPTILGIEHGNRLLWTVCIAALPFFWMTFGYHVWRRICP
                     LAVAGQIGRLVGKPGARKMGDWMGKNYLLVQLGSMIAALSLRLIVTNGSAIGVSVFLG
                     VVVLTAIVVSFVYGGKTWCNFMCPVGLVEKIYTEPARSAVGAAPETASQCTPCVACKK
                     HCPDIDLEQGYWKEVPERDTRGRRLAYFAWPGIVVAFYTYYFLARGTWDYYFDGRWAY
                     ERDVLAAEPGFYFWHAIPRAVAAPVTLIAFGLASYVVFASIERIALRIRKPSVDATEE
                     QKEQHTLRVRHGMLALCGLIAFNAFYCFAGQPTLQKLPAWVVTGWGLIVVFASTTIFM
                     RRISRREDQYV"
BASE COUNT         5109 a         9106 c         9256 g         4733 t
ORIGIN      
        1 gctgacaaga agatgactgc gaagctgacc ctccccgacg gcaaggagag caccttgccc
       61 atcatcgtcg ggtcggagga cgaacacgcg atcgacatcc ggtccctccg caacgagacc
      121 ggctacatca cgctcgacag cggctacatg aacaccggct cgacgacgag cgcgatcacg
      181 tacctcgacg gcgaacaagg catcctgcgc tatcgcgggt attcgatcga agacctcgcc
      241 gagaagtcga acttcgtcga ggtgtcgtac ctgctgatct acggcaagct gccgaaccgg
      301 ggcgagcgcg aggtgttcgg cacgatgctg actcggcaca gcctcatcca cgaggacatg
      361 caccacttct tcgccggcta cccgccgacc agccacccga tggccgtgct gtcgtcgatg
      421 gtcacgagca tgtccgcgta ctacccggac tcgctcgacc gcaacagcgc gatcgacctc
      481 cacatcacgc gcatcctctc gaaggtccgc acgatcgcgg cgttcgcgta caagaagtcg
      541 atcggccagc cgctcatgta cccgaagaac tcgctgcgtt actgcgcgaa cttcctccac
      601 atgatgttcg cggtcccgag cgaagaatac gaagtctcgc cgatcctcga gaaggcagtg
      661 aaccacctgc tcatcctgca cgccgatcac gagcagaact gctcgacgtc gacggtccgc
      721 atggtcggca gctcgggcgc cagcatctac gcggcgatcg ccgccggcat actcgctctc
      781 tggggaccgc tgcacggcgg cgcgaaccaa gccgtcatcg agatgctcga ggagatccgc
      841 gcgagcggct cggactacaa gaagttcatc gacgacgtga agaacaagcg cgacaaccgc
      901 cgcctgatgg gcttcggtca ccgcgtgtac aagaacttcg atccgcgcgc gaagatcctc
      961 aagaagatgt gcgacgaggt cctgggccac atgggcatca aggatccgct gctcgacatc
     1021 gcgaagaaca tggaagagat cgcgctcaag gacgactact tcatctcgaa gaagctctat
     1081 ccgaacgtcg acttctactc cggcatcatc taccgcgcgc tcggcatccc gacgaacatg
     1141 ttcacggtga tgttcgcgct cggccgcctc cccggctgga ttgcccactg gaaagagatg
     1201 gtcgaggacc cgcagacgaa gatcggtcgt ccgcgccagg tctacaccgg cagcaagctc
     1261 cgcaagttcg tcgcgctaga acaacgctaa gcggtgatgg gcaaggggcg gctcgaggcg
     1321 ttctccgacg gcgtcatcgc catcatcatc acgatcatgg tgctcgagct gaaggtgccg
     1381 cacggcactt cgttcgaggc gcttcggcac gtatggccgc cgctcgccgc gtacgtcgtc
     1441 agcttcatct acgtcggcat ctactggaac aaccaccacc acctggtcca cacggtgaag
     1501 cacgtggacg gctgggtcat gtgggcgaac atgcatctgt tgttctggct gtcgctgatc
     1561 ccgaaggcga ccgagtgggt cggcgaggat ctgcacgagc atgtaccgct cgccgtgtac
     1621 gcgttcgtca tgctgatggc cggggtctcg tacttgctcc tgacgaaagc gatcttgcgc
     1681 cagggcgaca accctcagct caaagaagcg ctcggcaagg actacaaggg caacctgtcc
     1741 ggcgtgctct acgcggcggg cgtcggcctc gcgttcgtgt cgcgctggat cacgatcgcg
     1801 atcatcatcg cggtcgcaat cctctggctg attccggatc gccgcctcgt gaaggccgcg
     1861 gcgaagtagc tacttcttgc agctcgtcgg caggacgtgc gtcttgtact cgccctcgta
     1921 gttgcccggc ggatcgtagt tgcagacgta gatgtcgttg cccttgcact gcacgtggcc
     1981 gcagccgact tgcttcgtgg tcgtccacac gagctgcgtg aagtggcccg tctgcatcga
     2041 gaagccgccg tcgggaaact tgtagagctt gatctcgtcg taccacatgg cgacggtgct
     2101 ctccggatcg agcgcgccga tggtgccggc cgcgaggttc tcgccgtact tcatgttgcc
     2161 gctgtggccg aacacgcagc ccttcgcttt gagctgatcg gcccacgcct gcgcgacctt
     2221 cgcgatctgc ggcgaccacg tcagcggcgg cgcgcagttc ttcgcgcgag cggcgttgtg
     2281 cgcgtcgacg aacgcctttg cggcaggatc gcttggtcct ttcgcggcct cggctttcgt
     2341 cgccggcttc tccgccgctt tctcgacggg cttcgacgtt ggcaacggct tcgccggcgc
     2401 gggcgcctcg acgtcatcgg gtgcggccgt ggattcctgc gaagcaggct tgctgtacat
     2461 ctgtccgcac gacgcgagca gcatcaggag ccagcgcatg cggcgagcat ggcacggcgc
     2521 cttcgagatg cccgttccgc aaaagttctt catgagccga tcgtgacgag gaacacgtag
     2581 cggtaatcga cgggcgtcgt cgtgtagaag cacgccggcc ctgctctgcc gaccgcggcc
     2641 gtcatcgcgt tcgagactgc ggtgccctgc gtgaagcgac agcggaactg cgagccgatc
     2701 agcgtgccct tcatctcgat gtgttggccc actccgagat ttcctgcaaa gcccgtcggc
     2761 gacgaaatct ggcccgggct tccgttgtac tggctcgccg cacgcacgct cggtccggcg
     2821 gcgttcgatg cggcacagca gtagtactgc acgctcggct ggacatccga gaggcagaag
     2881 ccgacctcgt tcgtgttcgg ctgcgtgatc tggatgctcg tgtagaagta cgcgtcggtc
     2941 gtgtacgtca gcggcgagtc gagcaccgag aagccgttca tcgtttcgtg cagcaggttg
     3001 ttcgagacat tccagacacc gacgctgttc tgcggctcgg tccacccggc gatctccgac
     3061 ggatcgtaga acgccatcca cagcgcgcga tgctcaccgg gactgagcgg ccgctcgtca
     3121 cattcgtcgc cgacgccgtc gtcatcctga tcgatgttcg ccgacgcgat gtgcgggcac
     3181 aggtcgcacg aatcgccgag gccatcgccg tcgaaatcgt ggacgtcgca cggcggtccg
     3241 ctatcgcgca tcgcgtcgtt gccgtcaccg ccaccgccgc caccatcgcc cgtcgacaac
     3301 gagccatgct cgaactggca ggccgcgagc agcaggaaca gcagcgcgcg catcacttct
     3361 tcttcttcgc cgccggcttc ttctttgtgg gtttcttggc cggcgccgcg ggcgcgcctc
     3421 cctcgagctg tttcaagaac ttcttcggcg cctgcgcgcg atagctctcg tcgatccatt
     3481 ccttcagcat ctcgaccggc agcggcctac cttcgggaaa ctgggcgctc acccaaccgg
     3541 acttgccgag gccgtacgcc gttggcttgc agaacggcag catgagcgcg accgacgatg
     3601 actgcgggag cttgcacgag atcgagaacg gctcgccctc gacgctcaga taggcgtacg
     3661 tcttgtcgtt gaccgcgagg tccatgtggc cgggccacgg gctcttcagc gccgtgccgg
     3721 gataggtggc aagaccccag tcgcggagct ccttcaccgt cttgtccgtg ggcaagctct
     3781 tcttctttgc catgcgccga gtgtaaattg ccccggtgcc acgaccaacg gttgccgagt
     3841 cgacatcgtc tgcggtgacg caaggcatcc gcgtccgggt gcagtcgcac tatttgcccg
     3901 aacagtccaa gcccaacgaa gaccactacg tattcgcgta cacgatcacg atcgcgaacg
     3961 agagcacgta caccgcacag ctacggacgc ggcactggat catcaccgac ggtcgcggcg
     4021 aagtcgaaga ggtcaaaggc gatggcgttg tcggggagca gcctcggctc tcgccgggtc
     4081 agagcttcca gtacacgtcc ggctgcgtgc tcacgacgcc gatcggcacg atgcagggga
     4141 cgtatcggtt ctggcgggac gacggcacct acttcgacgc gcagatcgct gcgttctcgc
     4201 tgtcgatgcc gctccaagag cgcaacgaag aagcgaattg atcccgctac gttggcctcg
     4261 tgggccgatg taggcaggaa aatctcaaag ccttcacgta cgctatgcgt ctaacgctgc
     4321 atcagatgac gaacgcggag cgcgaggcgg caacagccac cgccctggtg gaggcggcga
     4381 aggggggcga cgataaagcg ttcgaagcgc tcgtccgccg ctatcgcaag cgcatctacg
     4441 ccctcgccct gcacatgacg cgctcggcgt ccgaggccga cgacatcacg caagaggtct
     4501 tcctcaaggc gtaccgcgcg ctgcccggct tcgagggccg cagccagttc ttcacgtggg
     4561 tctaccggat gacggtcaat cgctcgctca acgcgaagcg cgatcgcgcc cgccgcggtg
     4621 aggacaccat cgatgatcct cgtctcgagc tcgccgtcgc cgtcgatgcg cgcggtaatc
     4681 cggcgcgcga ggccgagctt cgccagacgt acagccgcct gctgcgcgcg ctcgatcagc
     4741 tgccaatcga gatgcgcacg accgtgattc tcgtctcgct gcaggggatg tcccacggcg
     4801 aggtcggcgt gattcagaag gtcagcgaag gcacgattgc gtggcgcatg cacgaagcgc
     4861 gtcgccgcct gcacgaggcg atggttcccg agaaggtgcg ccgcccgcgt ggcgagctgt
     4921 cgtccgagct gtcgcgggtg ctgaccgagt acggtctgcc gctgattccg gtcaagggtc
     4981 gcgcgtagcc gcaaccggcg ttacgatgat cgcgtgcgat tcgcgatcgc gttgttgatc
     5041 gtcgcggcat gcggagacga aggccccgtc gctgatccgt atcagtgcgt cgcgagtggc
     5101 ggcgaggcgt gcttcgagct gccgtcgtat cgcatccgag cggcggaccc gatgggcgtg
     5161 ctcgccgagc cggcgctgga ctgcgcacca ttttcaccat cgagcctcgg tgacgcgacg
     5221 ttcagtgggc acaccgccga ttacgcgagc atcgtggggg acgtgcgcgt cgaggtgttc
     5281 agcgatgtcg cgatgaccga cagcctcgca gcggtgcaat cggatgccga cggcgtgttc
     5341 tccgcagaca tcgccgcgat gccgaaccag atgttcgtcc gcacgacggc ggatggccgg
     5401 ttgccgacgc tgcgcttgta tcaacgcttc gatgttgcac cgacgggcta ctacgcgacg
     5461 acgctcgccg acgcggacgt gacatcgatg ctcgcaggtc ccggcgatcg gcggctgccc
     5521 ggcaagagcc agctgcgcgg tatcgtcaac gactgcgccg gtaagcgact gatcaacgtg
     5581 gtcgcgaata tcgcaccgtc gtcgggcaag aatgggtggc ggctcttcga gcccggcgtg
     5641 cgcacgtact accagagcga cttcgacggc agctttctgc gccgcacggt cggctcgcag
     5701 acgacgcccg ctggaatctt tctcgcgacc aacctcgcgc ccggtcacca cttcgtgcag
     5761 gtgtggggct tcttgaccga cgaggatcta atgctcggaa gtctcgggct gaagctcctc
     5821 gccgaggacg agatcgtcgt tcccgacgcc gacagcgtcg tcgtcacgca cgagtacgcg
     5881 cggctctagt ccatcacgtc gtacgggccg cccttgtcga gcgctgcctt gaacgcgggc
     5941 cgcccgtgga tgcgcgcaac gtagtcgtga atcaccttgg gcgcgtctgg aatgcgcgcc
     6001 gcgatcgctt ccatcgggaa gctcatctgg atatccgcga gcgtgagctc ggcgccgcag
     6061 aaatacgggc gcgagccgag ctcgttcgcg atgaacggga cgtgccggtc gacgttcggc
     6121 ttcacgaatg cagcgtcgag cttgccagcg acggctttac cgacgagctt cgcgacaccc
     6181 ggcgcctcgc gcagtcgcct cgtgatgagc ttcacgagta ggatcggcat caccgagcct
     6241 tcggcgtagt gcatgaagta gcggtagcgc tggtactcgg gcgagctctt cgcggggatg
     6301 agcttgccgt tgccgtactt ctcggcgacg tactcgagaa tcgcggcgga ctccgcgacg
     6361 gtcgtaccgt catcggtgat cacgggcgac ttgccgagcg ggtggatctt ctgcagctcc
     6421 ggcggcccga ggctcgtctt cggatcgcgc gcgtacttca cgatctcgta ggggaccgcg
     6481 agctcctcga gcagccacag gacgcgttgc gagcgcgagt tctcgaggtg atggacggtg
     6541 agcatctcca gcggttgtag tacgtgacga gccgtagtta gtatccgcgg catgcgattc
     6601 ctactcgccg gcgtgctggt agttgcgggc tgctcgcagc tgatgccagc tccgaagcaa
     6661 gaaccgtttc cgttgttgga ggtgaaagcg acgcgtccgc cgcccggacc ggcgcgcgtg
     6721 gtgctcacgc cgtcgaacgt cgcgatcatg gacaaggtgc agttcgaaac gggcaaggca
     6781 gacatcaagc ccgagtcgca cggcctactc gacgaagtcg cgaaggtgct caaggagaac
     6841 ccgcaggtcg agcaaatctc cgtcgagggt cacaccgatt cgacgggctc gcccgagttc
     6901 aaccgcaagc tgtcccagca gcgcgctgag tccgtcgcga agtatttgtc cggcaaaggc
     6961 gtgaaggcag cgcgcatgga gccgaagggc tttggccccg accgcccgat cgccgacaac
     7021 gaatccgacg cgggtcgcga ggccaaccgc cgtgtcgagt tcaacatcct caagcagggc
     7081 ccgaagaaga cgttggtgaa agatgaataa ggcaacgctc atcatcgcag ccctgacgct
     7141 cgcgtccgtc gccgcatgcg cgaagaaggg catgtcgccc gccgtccgca gcgagatcga
     7201 gacgaagctc gccgcgacgc agaagccgat cgaggagtgc taccagcgcc agctcacggt
     7261 gaaccgcaag ctgcagggca tggtcgtcgc gacggccgcg atccaacccg acggcacgtt
     7321 ctccgaggtc acgctgcgcc gcgacgagcc gcaggatccg gtcctgaagt tctgcgtcgt
     7381 gcaggagatc gcgaagatca agctcgacaa gccgatgggc gagcgcgtcg tgctcgactc
     7441 gattccgatc aagttcgtct gggcgaatcc gtagtggcga agcttccaac gcgcagcccg
     7501 acgcacgaag aggtcgatcc aacgcggctc gcatcgatcg gtctcgagct cgaagagatt
     7561 gccgacggcg aggagattcc cctgcccgcg ctcgtcgagc tcgcggaaga tcgcggccgc
     7621 ccggcgagcc actacctcgc ggcaatcccg ctcgcgacgg agctgcgcct tgcgggcaaa
     7681 gcgccgatca gcgttcgcgt gtgtgcgggc aactgccagc gctacggcgc gctcgacttg
     7741 ctcgacgccc tggtcgagaa accaggcatc cagatcgtcc cggtgacgtg cctcgatcgc
     7801 tgcgatcaag cgcccgcatg cgaggtgcac agcgcgagcg ggcagctcgt cgtcgcgccc
     7861 gcgtcgaagt cgaacctcga cgaagcgctc gccgcgctcg cataactgtc tgcttttccg
     7921 acaggttgtc gcaggtgtcg cagcgttcgc gccctggccc gcgccgtgag cccgcgctag
     7981 cctcggctcg tcgtgttgcg cctgcacaag gtcaacaagt actaccaggt cggcacgcac
     8041 agccttcacg tgctgcgcga tatcgagctc gatattgccg acggcgagtt cgtcgcgatc
     8101 atgggcgcgt ctggatcggg aaagagcacg ctgctcaaca tcctcggcat cctcgacgac
     8161 tacgactcgg gcgagtactg gctcggcgac acgctgattc gcaatctcaa cgagagcaag
     8221 tccgcggcgt atcgcaaccg cttcatcggc ttcgtctttc agtcgttcaa tctgctgccg
     8281 ttcaagaccg cgatcgagaa cgtcgcgctg ccgctctatt accagggcgt ctcgcgccgc
     8341 aagcgcaacc agatcgcgat gcagtacctc gagcgcgtcg gcctgcgcga ttgggccgat
     8401 cacttgccgt cggagatgtc gggcggtcag aagcaacgcg tcgcgatcgc gcgtgcgttg
     8461 atcggcaagc cgcgcctgat cctcgccgat gagccgacgg gcgcgctcga cagcgagacg
     8521 agccgtcaga tcatggatct gatgcacgag atcaaccaga ccggcatcac ggtgctgatc
     8581 gtgacgcacg atccggaagt cgcagcgcgg tgccagcgga cgatccgctt gcgcgacggc
     8641 aaggtcatgg gccctggaga tgcggcgagc gtgagcaatc ccggaatgcc ggcggtcacg
     8701 ggctgatgtt tgtcgcgatc ctcaagctcg tcggtctgct ggtcggactc gccgtcgtac
     8761 tcgcgctgct cgcgacgctc gtggtcatcg cgatcgcgct cgtgcgcatg ttgttcgtga
     8821 cgctcgtgca gtggcgcgac ttgttctcga tcgaccgctg gcaggaagtg ctcgtcacga
     8881 tcggccgcaa caagctgcgc accgcgctga cgatgatcag cgtcgcgtgg ggcatcatgg
     8941 tgctggtcag cttgctcggc ctcggcgtgg gtctcgacca aggcatgcgc aagcagttcg
     9001 cccgagacgc gacgaacggc gtgtggatca gcgcgaacaa gacatcggtc gcgtatggcg
     9061 gctacgacgt cggccgcaag gtcatgttcg agaacggcga ctacgagcgg gcgaagaaga
     9121 tcgaaggcat cgagtacatc accgcgcagc actacatcgg cgggcgccgc tggggcgcaa
     9181 acctcgtcac gaagcgcgga gcgaaggcga acaccttcga gctgcaggcc gtgcacgcgc
     9241 cggtgtttca cctcgaagcg aacgagatga tcgtcggccg cttcctgagc gatgccgaca
     9301 tcgcgcagcg tcgcaaagcg gccgtcatcg gcaagaacgt cgcggatttt ctgttcggag
     9361 gcgagccggc ggtcggtgat tggatcacga ttggcggcgt cgcatttcaa gtcgtcggcg
     9421 tgttcaccaa tcccggcggc gccgaggaag aacgcaagat cttcatcccg atcacgaccg
     9481 cgcagctctc ctacaacggc ggcgagaaag tcggctcgat ccagttcacc gtcggcaacg
     9541 cgggacccga agagacgcag cacatcatcg atcaagttgt cggtcagctc gcggatcgct
     9601 ggagcttcga tccgaacgac aagcaggccg tgcgcgtcca caacaacatc gagcagttcg
     9661 agcgcttcaa gaagctgttc tggatgatct cgatgttcgt catcgtgatc ggtctcggca
     9721 cgctagccgc gggtgtcgtc ggcgtctcga acatcatgat gatcgcggtc aaagagcgga
     9781 cgaaggagat tggcgtccgc aaggcgctcg gcgccacgcc gtcatcgatc gtgttcatgg
     9841 tgatccaaga ggcggtcttc ctcaccgcgc tcgctggcct gttcgggctc gtcggcggtc
     9901 tcgtgatcct cgagggcctc ggcaagatga acaatgactt catcctcaat ccgagcatcg
     9961 acctccgcac cggcatcggc gcgacgatct tcctcgtgat tgccggcgcg ctcgctggct
    10021 acttccctgc ccgcgccgct gcgcgcgtca atccgatcca tgcccttaga gacgaatgag
    10081 ttggatgagt agcgatgttc gatttggata agtggaaaga gatctgggcg acgcttcgca
    10141 gcaatcgcct gcgcacgttc ctcaccgcgt tcggcgtgtt ctggggaatc ttgatgctga
    10201 tggcgatgct cggcttcggc tcgtcgatcc agacgggcag ccagaagcag atgcgcggca
    10261 tggcgacgaa cctcctgttc gtgtggggcc agcagacgac ggagagctac gacggtctgc
    10321 cgcccggccg gcaggtgaag ttcgacatcg acgacatcga gctgctgcgc cggcttcccg
    10381 gcgtcgagca ccttgccccg cgcctgcagc tcggcggctg gatgaacaac ttcacggtcc
    10441 agcacgacac gaagagcggc acgttcacgg tgatggccga ctatcccgag ctcaagtaca
    10501 tcatcgcgtt cgagtacgag gcaggtcgct tcatcaacga gcgcgatatc gccgagagcc
    10561 gcaaggtcgt cgtcatcggc aaggcgatcc aagagcaact gttcgcgccc ggcgaagatc
    10621 ccatcggcca gtacctcaag atcagcggcg tgtacttcca ggtcgtcggc gtcacgaaga
    10681 cgttgcgcac cggccagcaa ggcgagcgcg acgcgaacag cgcgttcgtt ccgttcacga
    10741 cgctgaagac ggccttcaac atgggcaacc gcgtcggctt cttcgcgatg accgcgaagc
    10801 ccggcaccga tggtcccgag ctcgagcggc aggtgcgtgg tgcactcgcg caacatcacc
    10861 gcatcgcgcc aaacgatcag ctcgcgatcg gctcgttcaa catgttcgtc atgttcggca
    10921 agttcacgac gttcttcttc gtgctgaagg cgctgtcgtg gatcgtcggc ggcctgacgc
    10981 tgctcgcagg cgtcgtcggc gtctcgaaca tcatgctgat caccgtgaag gaacgaacga
    11041 aagaaatcgg cgtgcgcaaa gcgctaggcg cgacgccgat gtcggtcatc tcgatggtga
    11101 tgaaagagtc gatcgtgctg acgctgatcg cggggctcat cggcgtctcg gtaggcgtcg
    11161 ctctactcgc gggcgccgag atggcgctca agaacgcgac cgactcgccg ctcggcccgc
    11221 ctcacgtcga cctctcgacg gtgctcgtcg cggtcggagt tctcgtcgtt gccggtgcgc
    11281 tcgccggcat catgcctgct tctcacgccg cttcgatcaa accgatcgag gcattacggg
    11341 cggagtgact atgaagaaga tccttacctg gttgattgcg gccgtcgtca ttctcttcat
    11401 cggcggcgcg ttcgtgttcc tctacaagaa gtcgcaggcc aagcccgtca cgttccagac
    11461 ggagactgct gactacgcgg acatcacgaa gaagacggtc gcgaccggct cgatcgttcc
    11521 gcgccgcgag gtcgaggtga agccgaaggt caccggcgtg ctgtccgagc tgtacgtcga
    11581 gcccggcaag aaggtgaaga ccggcgatgc gctcggcaag atctcgatca ttcccgacgc
    11641 gatgcagaca cagcaggcgc agaccggcgt gaaggccgcg cagatcgcat tcgacaacgc
    11701 gaagcgcgag ctgcagcgca acgaggagct ctacaagaag ggcgtcgtcg ccgatgcaga
    11761 gctgcagcgc taccggaccg aattcgcgct ccgccagcaa gagctcgagg ccgccggcgg
    11821 ccggttgcag ctcgtcaaag agggcgcgac gcgcggccag ggctcccagt cgacgctgat
    11881 cgtcacctcg acggccgacg gcatggttat cgacgttccc gtgaaggtcg gcttctcggt
    11941 gattcagtcg aacaacttca atcccggcac gacggtcgcg acggtcgccg atatggacga
    12001 catgatcttc gacgggcgcg tcgacgaggc cgaggttgcg aaaatcaagg agggcatgaa
    12061 gctctcgatc aaggtcggcg cgatcgagaa ggacacactc gagggcgtgc tcgagtacat
    12121 ctcgccgcag ggcaagacga tcgacggcgc tatccagttc cagatcaagg cggccgtgaa
    12181 gcagaagccg gacgtcatga tgcgcgcgaa ctactcggcg aacgcggaca tcgtgctcga
    12241 cgagaaaaag catgtcctgg cggtccgcga ggcgctcgtc cagtacgaca acggcaagcc
    12301 gtacgtcgag gtcgagacct cgccgcagac gttcgtgaag aaggacgtcc agctcggcct
    12361 ctccgatggc atcaaggtcg aggtcctgtc gggggtcgca gcgacggaca agatcaagat
    12421 tcccgagacc gcgggtccgc cgtcggcgat gggctccgga ggtccgggcg gcaaaggcgg
    12481 cggaaagcct cccgcgaaga agtgaggcgc gggcgcttcg gctcggggat cgccggagac
    12541 cgattagggc tccacgccga cgcagaagtg atagcgtggc gtcgtgggca cgatgctcgg
    12601 ggcgatcgac gccggttcga atgcgatccg cgtcgtgatc gcagagctcg gcgttacgga
    12661 gctcgtccgc atcgaagccg agcgcatccc ggtccgcctc ggtcgcaacg cgttcacgcg
    12721 cggtgagctc gacgaaaaga ccatcgacga tgcggtcggc gcgttcgtgc attttcgcca
    12781 gcgcttcgac gcgcacggcg tcgtgctgta tcgcgcggtc gcgacgagcg ccgtccgcaa
    12841 cgcgagcaac cgcgatgtgc tgctccaccg cctgtatcac gaggccggca tcgagctcga
    12901 agtgatcgaa ggcgaggaag aagcgcgcct cgtccgcaaa gcagtgatgc agtcgctagg
    12961 accgcaagcg gtgccgcgcg cgatcctcga tctcggtggc ggcagcctcg aggtcaatct
    13021 caaggcgggc tcggtgtggc gcggtcactc cctgcccgtc ggcacggttc gcttgctcga
    13081 gacgttcggc ctcgatggcg cgatcgccga cgccgaagcc ggcatggtgc gccgctacac
    13141 cgcgacgctg atgcacacga tcgcgaagaa cgggccgcag tccggcaccg ccgcgatcac
    13201 gggcggcaac gcagaagcgc tcgcgcgcat catcgccaag gacggcagca acagcttcga
    13261 gctcgccgat ctcgagaagg cgctgcccga tatcgtgagc gccaccgtcg agcagcgcat
    13321 ggacaagctc ggcgtcaaac gagaccgcgc cgaggtcctc ggcatcgcat cgctcgtgtt
    13381 cgcgaccgcc gcgcgccagc tcggcttgca gaagctcgtc gcacccggcg tcggcgtgcg
    13441 cgaaggcgtg ctcatcgagc tcggcgagac cgcccgtgaa gaacaagcga aagccgaagg
    13501 tgcgcacgac aaggcgttgc tcaccgccgc gcgcagcttc gccaatcgcg tcgatcacga
    13561 cacgacgcac ggcgagcatg tccgcctgct ctctcgcgcg ctgttccacc agctgcgtga
    13621 cgtccacggc attcccgacg acaaaggcgt gctcctcgag gtcgctgcac tccttcacga
    13681 cgtcggcgag gtcgtgaacc agcgcggtca tcacaagcac agcgagtaca tgatccgctg
    13741 gggccgcatc ccagggctcg acgacgcggg ccgcgagatg gtcgccctga tggcgcgctg
    13801 ccaccgcaaa gacattggcc gagccaaaga gatgatcaat gcgtcgcagc tgccgaaaga
    13861 gcagcgcgtg cagcttcgca gaatgaccgc actcctgcgc atcgccgaca gcctcgatac
    13921 cgaccatcgg tcgcgcgtcg agcaggtcgt gtgcacgcgc atgggcgagg cgatcgtgct
    13981 cgacctcgtc gtccgcgacg ggccgacgcg cgaggaccct cgcctgctcc gcaagaccga
    14041 catgctcaag gacgagctcg gcctcgacgt tcgcgtcacc gtcgcccgtc ccgtcgctgt
    14101 gcccgtcgag gccggaacgt ccggcgagct gcgtgctgtg cgatcgcgca tgctctagcg
    14161 cggctcccta ccgtgccccc gaaggattga acagggagac acggagacac gaagtcggat
    14221 gcccaacggc acgtccgcgg cccgcatgcc ccggcacgtc cgcggccggc acaccccggc
    14281 aggtccgcgg ccggcacgcc ccgacccatc gcctgatctt cgacaggcac catcggatcc
    14341 cgaatcgatg ccacgggcac atgccctgcc cgcgcgaagc gcccctgacc tccgtgtctc
    14401 cgtgtctccg tgtttatcgt tcgggcacac gccctacctg tcgacgcgcg ccgaggcgga
    14461 tgtcaccgcg cgcgcggctg ctgccgtgat atgcccggcg catgcgtggt gcgctgctcg
    14521 tctgcttgac ccttgccgcc tgcggagacg atccgccgac cggaccgctc gcgtatcaca
    14581 tcacgcacta cgactacacg ttcgacgtcg agtcgcgcgc cgcgcacgcc gaggtcacac
    14641 tcgcggtcga tacgggtggc gactgcttcg acattccgtt tcgcgcagac ggcgctgcga
    14701 acgcgacgat cgacggcgac gcggtcagcg ctgcacccga tggcacgaag ctgatggtgt
    14761 gcggcaccgg ctacgagacg ggctcgacga tcaagctcgg catcgatgtg cagatcaagc
    14821 tcgcgacggt cgggccgagc caggtcggct actcggtcac gcaggacacc gacggcaaca
    14881 agctctacta cctcgtgagc tgggtcggcg gctgcgatca gttcggcccg tgcgacagcc
    14941 gtcccgatca gttcgcgacg tacaagttca ccgtcacgca tccggcgagc ttcaagacgc
    15001 gctgccccgg cacgatcacc gaaccgagcg cgacgcagac cgtgtgcgac ttcacgctcg
    15061 ccggcggacc gacgtactcg acgtttggcg tcgcgacgta ccccgcgtgg accgagagcg
    15121 acaagggcac gtggggcggc atcaaggtca cgctgtacga ccgcgcctcg acgacgatca
    15181 acgccgcgat cgattcggcg tggcacgacg gctacatgac gtggatgcag gcgacgttcg
    15241 gcccgtatcc attcggcaac gagctgcgcg tgctcaccgc gccgacgtac tggagcggct
    15301 tcgagcatcc cggcaacatc gtcctcgacg attcgctcgc gaagctcacg cgccctcgct
    15361 actggagcga gacggcgcac gtcctcgatc acgagatcgc gcatcagtgg gccggcgatc
    15421 aaacgacgct gaaggacacc tacgacttcg tctggaagga gtcgatggcc gagtacctcg
    15481 catacgtgta cgaggacatg caggacccgg cgaagtcgct cgtgaccgct cgctactgga
    15541 agactgcggc cgcagctgcg gagtatttcc cggtgcccgg cgagaagccc gcactgttcg
    15601 actactacgg cgatgtctac ggcgaaggcc cgatgatcct cttccaccag ttggagacga
    15661 tgtacggccg cgacaaggtg atcgctgcgc tccagaaagt gctcggcaag gagcaggcga
    15721 tcggcgtcga tgatgtgatc gccgcgttgc aggagacgac cggcacggat ctgtccgcgt
    15781 acgcagcagc gtggatccac ggcagtggcg cgccggattg gccgaagtac aacgtgacgt
    15841 tcacgcccgg cagcctgtcg ctcacgatga tgaacaagaa gtcgacgccg cgcggctgca
    15901 tgttccacgt cgcgctcgaa ggtgcgaaca cgggcgagtc actcgtgatg acggtcgaca
    15961 cgcgcacggc gcaggaccaa acgatcccgg tcgcaccgct cagcttcacg gtgacaaagg
    16021 tgacgctcga tccgtatgcc gagtgcctcg tctacaaggc atcgggcaca ccacgcatga
    16081 ttcgcgagaa tccgtgggtc agtgagcact gaacgcacgc tcccgtgaga tcgctgtttt
    16141 tctggcgcac gatccgcgta tcgaatgcgc ctttttgata tccacgctcc aggaaaagag
    16201 gagcttcgaa caatgcgtgg atattcgctt ttggtagctg cagtgctcat gttggcgtcg
    16261 tgcggtggca agttgcccgg cggcaaaggc gtccccggcg gcgacaagat tccgggcggc
    16321 ggcaatgttc cgggtggcct cggtggtgac tccggcatgg tcgatccgaa cacctgcggc
    16381 aactacgccg gcatggaagc gggcgcgcgc ctcaaggcgt tcctctcggc ggtcgccgat
    16441 ctcgacaagc aggcgaagga gaccgtcgag gtcgttcgca ccagctgcaa gatgatgggc
    16501 aaagagctcg gcatgagcga cgccgacttc accgacggca tggagacgaa ggacgtctgc
    16561 gccaaggtgt ggggcgccta caacgacaac atgaaggtcg cggtcaagag caaggccgcc
    16621 ttcaaggtca agtacacgcc ggccgtctgc aagctctcgg tcaacgcggt ggccgaggct
    16681 gctgcgaagt gcgaaggcaa ggcgagcgcc gacatgggcg cgacgtgctc gggtacgtgc
    16741 aacggcaagt gcagcggcaa gtgcgcgggc tcgggtaagg ccggcacggg cggcagcagc
    16801 ggctcgggcg agtgccacgg tcagtgcagc ggcacgtgcg aaggcgactg cgaaggtcac
    16861 gcggacgtga aggcgtcggg ccagtgcaag gcgcacgcgc aggtcgaggc gtcggcgaag
    16921 atggagtgca ccgaggccaa gctcgaggtg accctcgacg cgaagctcgt cgtcgacaag
    16981 tcgaaggcgg agcagacgct caacgcgatg aagaacggcc tgccgaagct gctctcgctc
    17041 aacgcgcgtc tgaagccgct ccaggcggcc gtcgagacga cggtgtcgac cgcgaaggag
    17101 ctcaagaaca tgggcccgaa gttcgtcaac tcgttcaagg accaggcgct ctgcatcagc
    17161 ggccaggtcg ctgcggcgct caacgctgcg atgagcatcc aggcgaacgt gtcggtctcc
    17221 gtctcggtct cggccgaggc gagcggctcg gtcggcggcg gctgattcgt tagccccgat
    17281 ttgcgacgcc ggcgagggtt caaaccttcg tcggcgttgt cgttttcggg ggcatgttgg
    17341 gcctggcgcc gggtgcctac ttggtgtagc tagcctgtgc catgaagacg ctgaccgagt
    17401 tctcgccgct tctgttgcgc aatgcggccg ctgcacgcaa agccgctggt acgggctccc
    17461 ccgcgccggc cgcatccgaa gaagcgcccg ccgcagaagc accgcccgct gccgaagagg
    17521 ccgctgcgga agcagcgccg gcggaaggtg atgcgtctgc cgaagcgtca gcggatgcgt
    17581 cagccgaagc gtcggccgat gcgtcgagcg aagcgtcgga aggtgaggcc gtcgaagcat
    17641 cgggcgaaga gcccgagccg atgaaagaag aaggcgagcc gaaagccgat ggcggtgcag
    17701 cgaaggctcc cgatccggcg atcgagccgg tcgccgcagc actcggtgtg caagcggatc
    17761 gcgcagcgcg tctgctcgag gctctcgaca tcatcggcga tcgcatcgat caagttcgtc
    17821 tcgtgcgcgt gttccaaggc gagaagggac cgcacggcgc gatcacgcgc ggcgagttcc
    17881 actacgtgat cgatcgcgtc gctggtgcgc aacgcgcgcg acgtgatgat cgcggcggcg
    17941 atcgcggtgg acgcggcggt gatcgtggcg gacgtggcgg cggtggtgat cgcggtggac
    18001 gcggtggcgg tggtcccggc ggcggtggcg gcggtggcgg cttcggcggt cgtggcgatc
    18061 gcgacaagcc ccgcggcctc ggcagcctga agatgggcgc gccgaaagaa gagaagaagg
    18121 atgacgatcg tccggctcgt ggcgagatgc cgcgcgcagg aatcggttgg cagctcacgg
    18181 cagcgccgcg cgacttcagc gatcgcggtg gccgtggtgg cccgggacgc ggacgcggcg
    18241 atcgcgatcg tcgtggtccg ggtggcggac gcggtccagg tggtcccggc ggcggacgcg
    18301 gtccaggtgg tccgggcggc ggacgcggtc caggcggtgg acgtggtcgc ggacccggtg
    18361 gtccgggcgg cggacgcgat tttggtccgg gcggcggacg cgactttggt ccgggcggcg
    18421 gacagccggg cgtcgaaggc ggcgcgccgg gtggcgaagg cttcgctggt ggtggcggtg
    18481 gcggcggaga attccgcggt cgcggtcctc gtcgcggcgg cggtggcggt ggtggcggcc
    18541 cgcgtggtcc gcgccccgaa ggtcagaatg catccgggca gaatgcgggc ggcgaccgtc
    18601 ctccgcagca gccgcgtctc ggccccgatg gtcagccgtt gccgccgcgc agcaagcgcc
    18661 cacgcaagcc gattggtccc gacgcaaacg gcatgggacc cgacggaaag ccgtgggatc
    18721 ccgagcgtcg tgcgaagcgt caggccgagc gcgagacgcc cgtgaataac gagcagggat
    18781 cgtccgtcga aggccagacg ccgtcggctc cggtgcagaa tcagtccgaa acaccgaccg
    18841 aataaccggt tatcaaagtc acagaaaaag aaactctgag acggccgcgg tgtcgtggtt
    18901 ctattcttcg tgtttggagg ccctatgtcg cgtcattgga tgtccgcagc cgtaatcgcc
    18961 gtggccgtcg ctgcgaccac gcagcccgtg ttggccgaca tgtctcgtcc ggtcatctcg
    19021 gcgttcaaag gtcagctcgt cgtcagcaaa gacgagctcc ccaccggtaa gaacgacaag
    19081 gacacgatcg ccaagatcaa gaaggaacag ctcaaggagc tgaccggaac tcctggcgag
    19141 gacaacacgt cctggaactt ccactacacg gcgttcctct cgaagaccgg cgcgaagaac
    19201 ctcaagatgg agttcatgaa gggcgacaag ctcgccgcgg acaagcagct cgacggaatc
    19261 gatccgaaga gcaccgttct ctcgggcgac atctcgatcg acgagaacga aggtctcgcg
    19321 aagggcaaca cctatacgat caagttgctc gcaggctcgg ccgtcgtcgc gaagacgacg
    19381 ctgatcatga aatagctagt cgtagcgtcg gcgccgcagc acgatctgcg cgagcacgaa
    19441 caggccgaca gcgaagccaa catcatggcc gctgttgcag ccgccgatga ggtcgccgtc
    19501 atcgtcaccg tcaccgcctg cgccgtccgg cacgccgtcg ccgtcctcgt cggaaccgct
    19561 gccgctgccc gagccatcgc cactgccggc cttggcgacc gtgaccgtga tcgacttgcg
    19621 cgtcgggttc ttgccgtccg tggcctcgac gctgatcgtg tgcacgcctt cggcgagcgc
    19681 tgcgtcggtc gcgaacgtgt aggggcctgc gcctgccgcc gtcgtcacgg gctgatcgtc
    19741 gatgaagagc tgcgctgcgg tgaccgcgat gttgtcggat gccgtcgcgt cgaccgcgaa
    19801 gccgggctcg acggtcgcgc cgtcagcggg cgacgtgatc ccgagcgtcg gtgcgacgag
    19861 gtccgcttga ccgagccgct ggttgagcag ctgcacgctg ttttgattcg gccggcaggt
    19921 cgagccgccg atgccacacg ggcgattcga gtactcaccg cacgcgaccg tctgatcctt
    19981 gaacgtgcgg ttgccgttgt agttgagata cgtcatcgga tcggacgcca gcatctcgtg
    20041 gtcgagccca tagctgtgcg cgacctcctg cgccatcacc tcgcagactg tttgcggatc
    20101 gtcggggaac acgctcgtga acgtgaacac gatcgagttc tcgatcacgc cgcagtcttg
    20161 cgtgaacggg ctcacgccgc cgacgccctg ccccatgccg acgttctgcg gcgagccgcc
    20221 gaagaccgct tccatgtgcg ggacgttgcc tggatcgacg tcggtgacga cgacatccca
    20281 gcgcttgaac atgtccttca tgcacgtgac cgtctgctgc cagtgcgccg cgctcgtatt
    20341 ccatgcgggg acagacacct gctgcgagac gatcgacgat cggttcgtcc gtgcgtcgtc
    20401 gttgccggga cgaagcgtga cacccgtgtg gttgaggtag atgatcttgc tctgcgcgag
    20461 cgcgtagacc cccgacggcg gaatctcttt ccgcacgaac gtgatggcgc tcgggtccgg
    20521 cagatcgcga acggcctcgg ccgctgcgag cgacggaacg agcgacgcgg tgacagcccc
    20581 tgcgatgatc gtcgcgatgc gcatgcagcc gcactaatca cgcggcgtgc cacgtgactc
    20641 cagcgcacaa tctccgtcga caaaccgcgc gttgcacaac tcgcgcattg catcgtcgac
    20701 tgcgccgagg tgtcacgcga gcggggaaat gttctccgta aacgaactgc ctcatacgtg
    20761 tgaggcgccg atatcccacc ttgtccgctt gttgacggcc agtcaacgag gaacggcgag
    20821 gaggcggccg ccatagacgt ccgtcgcgta caccgtgtcc ttcgtgagtg cgatccggcc
    20881 agcgccgatg atgtctgacg cgagcgccgt gatcttgcga ttgcgatcga tgtggagcag
    20941 ctcgccgtcg tcgttggtcg agtggcccca gaggccgctc gtcgtaccgt acgtcagcac
    21001 gtagacgccg ccctcaccgt cggcgacgac ctcgcgcgga tcatcgaggt gctccgcgag
    21061 catctgccgc ggaccgccac gcgtcgacat cgtgtagatg ccgcggtccg tgctcgcgta
    21121 gatcgtatcg ccggcgacgt cgaagctcac gacgagcgct ccgaagtcag ccacctgctc
    21181 gagtggtccg cccgccagcg gcatccgcga gatcacgccg cgtctgtcgc cgacatacag
    21241 cgtgtctccg accgcccgaa tccggccttc gatcgcgccg agcggatgct ctgcacccga
    21301 gggctcgcgc acgaacgctc cgccggtccg ccagatcacg atggccgcgc ccgcaggggc
    21361 cacgtaggtc acgtcctcgg cgacgcggat cggatcggca tctggcaaga ggcgccacag
    21421 cgcggacgtc ttgccgccca gctcgtcatg cgcggcgatc cacgcacctt cctcgtctgc
    21481 ggcgatcgca ctcacccagc cgaggctctt cacgatgcgc ggcttgccgc ggtccagcag
    21541 catcacatcg cgattgctcg cgacgaccca tacgcggccg ccgccgatcg agagctccag
    21601 cgggtacgtc agcgttgccg cgacaggcgc gggccccgcc ttcgcccaat gcgcaggctc
    21661 cgagccgcta ccggacgacg acgaatgtcg acatgccgcg agcgcgagca acaagacgac
    21721 ggcccatcga caccgcatgg gtcgacggta gcgcagtcgc tctggcgcta tccggcaatc
    21781 ttgcgctgcg cgagtgcgtg caacgctgca tgcccgaggt cgctctggta cgcgggccga
    21841 tggtgcggcg cgtacgtgcg ttcgctcgag ggccgcgcaa caacgtcgct ttggtcaaag
    21901 cgatccgctg gagcgggcag cgccggtgcc acgtactcgg ggcgacgcgc ttccacttca
    21961 cggattgccg tccgcgagaa gtcgcgacca ccgaacgtgc gcaccggcgc tggcgacgaa
    22021 gcagcgagat tcttcagagc cgcacgcgcg atgatgctgc caccgccttt gggcctgaag
    22081 tccttcgctt gcagccggta cctattgagg agccggtgca tgctttgtcg ctcgatgcct
    22141 gcttgccgcg acgcgagagt cacgttgccc tgacactcgg tcagcagtgc ttcgagtgcg
    22201 ctgcgaatcg cagacgcatc atcggaggaa agaacagatc ggtcgaacat agtggttgcc
    22261 gacaagagca gccgacatgc caagcaatcg ctcgggtgat cggcaaatca ccgccgtgac
    22321 ttagctctac tggcaactcg actgtctatc tcctcaggta cacgcattgt cgcgacgggt
    22381 ttgcagaatc gctcccgccg ggatctcgcg gaagtccgcg tcaggcttgg gtatctcccg
    22441 cggtagtcag cgtgtctcga acgggttgca gattcgaagc gcccaccaaa aggacctcgc
    22501 gcatttgcgc gtggcacgcc gtctgctcaa tgggtttcgc aagcaagaag gagcatcccg
    22561 atgaagtcag gtttcgaaac gatcgaaagc aacgagctcg tcaccgtcgc cggcggcgcc
    22621 ggtggcacca gcgtcccgac cagctcgggc acgaatgacc agctgctctc cacggtgcaa
    22681 ggcatccaga gctcgctgaa ggatctctcg agcaaccaga acaaccaggg cctcttcaac
    22741 ggacccaacg gtgcactcgc cttcggcatg atggcgatgg cgatgcgtcg tccggcgagc
    22801 acgacggtcg tctatggcgg tcgtcgcggc ggctactact ggcagtcgtc gtggtgagaa
    22861 cgaacatgaa ccacttccaa acgatcgcca cgacctcgct cgccaccgtc acgggtggca
    22921 aggcgacgag cgcacaggac acgcagctca ccgcgatgct gaccgacatc agcaagtcga
    22981 tcaaagacgc ggccgaagcc aagaacaaga gctcgtctga tccgactcag atgatgatga
    23041 tgatgatgat gatgggcgga ggcggcggcg gctcgcagca agcggtcgca gcggctcctc
    23101 caccgccggc gccatcgccg aacgtcgtcc gcgtcaacgt ccgctgagtc tcagcgcacg
    23161 agcttgcgtg ctgccgcggc gacattcgcc ggcgtgatac cgaggtgctc gaagatgacc
    23221 tcggcaggcg ccgatgcgcc gtagcgatcg atgccgatcg cgatgccacg ctcgccgatg
    23281 tactggcgcc agccgaacgt cacgccggct tcgacggaca cgcgctgcca acgatcgggc
    23341 ggaagcacct gcgccttgta cgcggggtcc tgctcctcga acacttccca cgacggcatc
    23401 gagacgacac gcgcggagat gttctccttc gcgaggagct cgcgcgcgcc catggcgacc
    23461 gcaacttctg atcccgaggc catgaggatg acatcggtac cgtcggcgag gacgtaagca
    23521 ccgcgctcgg cgccgggcgc gcccttttgc gtgagcgtcg ggagatcttg gcgcgagagc
    23581 acgaggcccg tgggaccgtt gcggagtagc gcgagcttcc agccgaccgc ggtctcgttg
    23641 ccatcgcatg gacggaacag cgtcaggttg tgcatcgcgc gcagcgccat caggtgctcg
    23701 acgggctggt gcgtcgggcc gtcttcaccg aggccgacgg agtcgtgcgt ccacacgaag
    23761 atcgcgggct ggttgttgag cgcggcgagg cgcacggtcg ggcgcatgta gtcgctgaac
    23821 acgaagaacg tcgacacgta cgagcgcgct ccaccgtgat agagcatgcc gttgccgatc
    23881 gcgcccatcg cgtgctcgcg aatgccgaag cgcaggttgc gaccttcgcc cgctttgacg
    23941 taatcgccgc cggggacgat cgtcttcgtc gagccgccga ggtcggcgtc gccgccgaac
    24001 agccacggaa tcttcgccgt gatggccgcc atgactttgc cggagcacgc gcgcgtcgcc
    24061 atcgagcccg tgaacgacgg gaactcggca tcccagcccg gcggaagctc gccgttgact
    24121 gccatgtcga attgcgcggc gaggtcggga tgcgcggcct tgtacgccgt catcttctcg
    24181 cgccacgcag cgtgagcagc tgcaccgcgg cggccgccct cgctcatgtg ctggcgaact
    24241 tcgtcgggga cgaggaactt cgcgttcgga tcccagccga gtgcttgctt cgtcgcggcg
    24301 atttcggcgt cgccgagcgg cgagccgtgc gccgagctcg tgccggcttt cttcggcgag
    24361 ccgtagccga tcgtcgtgcg gatcacgatg atcgacggac gatcggtctc tgctttggca
    24421 gcggcgatcg cggcatcgag cgcggcgagg tccgtgtcgc cgttctcgac gtgctgcacg
    24481 tgccagccgc acgcggcgta gcgcgcgccg acgtcttcac tcatcgtcag gctcagcggg
    24541 ccgtcgagcg tgatttgatt cgcgtcgtag agatacgtga gcttgccaag gccgaggtgg
    24601 cctgcgagcg atgccgcttc gcacgcgacg ccttccatca cgtcgccgtc cgagacgagc
    24661 gcgaacgtgt ggtgatcgat gatcgtgtga ccggggcgat tgaagtagcg accgaggtat
    24721 cgctcggcga tcgccatgcc gaccgcattc gcggcgcctt gaccgagcgg gcccgtcgtc
    24781 gcctcgacgc ccggcgtgag gtgccattcg ggatggcccg gcgtcttgct gtcccactgg
    24841 cggaagttct tcagctcgtc gagcggaagc gcgtagcccg cgagatgcag cagcgagtag
    24901 agcaacatcg agccgtggcc cgccgacagc acgaagcgat cgcgatcgaa ccacagcggc
    24961 gacttcggat cgtgcttgag gtggcgttgc cacagtgtgt acgccatcgg cgcgcaaccc
    25021 atcggcagac ccgggtgtcc cgagtttgct ttctggatgg cgtccatcgc gagggtgcgg
    25081 atcgtgttga tcgacagcgt cggtagatcg gtcgtcatcg gcgagcggaa cctagcatgg
    25141 tggtacgatg ccgcggcatg cggagactca aagctctaat cctcgtaggt atctgggcgt
    25201 gtggcggcgg taagccggcg acgcagccgg tgccgattac gccgcctgcg ccaactcccg
    25261 tcccaactgc gtcgccgacg cccactccga ctccgacgcc gactcccgcg ccaaccgccg
    25321 agaagccgtt cactgtcgac gacaagagtg ggcccgagtt cttcccgcct gcgcgcaaga
    25381 aggtcgcgat cggtcagacg atcagctttt cgacggcgat catcgatcag gatctcgacg
    25441 agacgcgcgt cgaagtcacg cgcatgccaa agaccgcgac gtacgacgcg atcacgcaga
    25501 cagtgacgtg gacgccgggc gccgacgagg cgtacacgct accgaagggc tctggtctca
    25561 aggagaaggc gcttccgcag ttcgagctca gtgttacgca gcccggccgc gagaagaccg
    25621 cgaagtacga agtgctcttc gaggtggtga agggcaagcc gatcgccgag cccgtcgcgc
    25681 cgcagcagac cgccgtgatc gagacgctgc tgatgatccg gtcgcccaag cgcctcgagc
    25741 aggtcaacaa ggactggccg ctcgacaaga tgctcgcggt cggcgccgag acattcaagc
    25801 tgcagattcc cgaggacaag cgcagcaagc tgaagggctc gctcgacggc aatacgtcgt
    25861 acacgcagat gctcgcgggc ctcgcgcaga cgcacaacaa tccgcgcctc gatccgacgt
    25921 cgccgcagtt cgacaaggcc gcgttcggca gccccgccga ctggaagatc gtcacgtttc
    25981 gcccgcgcat cgatcgcgcg tggaccgagc tgcgcgtcgt ttatcgcgcc gtgaaggcgc
    26041 aggaaccggt gttcgcgatg ttccggctgc ggcccgtcgt cgagtacgtg cctgcgctgc
    26101 cgcgtcccga ggaagagcac gaggcgaaca acaagatctt cctcgggatg gtcgcgaagc
    26161 acctgatgaa gaacggcggc cccaatccga agttcgtcaa agacgaagcg gcagagggca
    26221 aggcagtctc tgcgctgctc accgagctga tgtcgttcga cgacacgaag acaaagccgt
    26281 acctgcatgg cttcgagatc ggcatcgcac tcgaggcgcg gatgggtggc ggctcggccc
    26341 gcaacgccga tggctcgtac aagagtggcg acggttgggg ctggagcgcg cagaagccgt
    26401 tccagacgag cgaaggctcg acgcaggtgt atcagaacgt cgcgattccg ggattctgga
    26461 cgcagacggt cgcgagcaag gacaacacga cgtgggtccc gcgctgtgcg ccgcgcttca
    26521 atcccgagga caagttcgtt cccggctacg aaatcctctg ccgcaagacg atgggcttcg
    26581 tcgatcttcc agcgacgaag gatggcaaga tcgtcaacgg ccgcgtcgac gcgaacaaca
    26641 tgtacttcga gcacaagaag cgctggatgg tggaaggctt cccactcgaa gacggccgtc
    26701 gcgacctcgg tgaagagaac ggcatgacct gctcgcagtg ccacattcgc aacttcggaa
    26761 tgcacgacta ttccgatccg gcgaacgtcg atccgtcgaa gggcacaccg aaggcgctga
    26821 acaagtcgct cgcgacgctg aacttccaga tcgtcccgag cgcgacgtgg gaagagttca
    26881 cgatcgactt tttgcatcac caggaatgcc gcggcaaaac gaatctcgag aagtacctcg
    26941 gagcagattc ggcgaagggc ctcacctgcc cgctcgcgcc gaaatagatc gacgtaatgg
    27001 ctgcacccgc cgcgcccaag aagaagcttc cgctcgcgcc ggccgatgac aagccggcga
    27061 agaagccgga aggcaaggcc gcgaagattc gcgccggcgg atggcatgca ttcaccgatg
    27121 cgggcaccgc gccgaagtcg ctgaccgctg cgcgcatcgt gatgacgctc gcgctcgcgg
    27181 cgacgattcc gacgatcctc ggcatcgagc acggcaatcg cctgctgtgg accgtatgca
    27241 tcgcggcgct gccgttcttc tggatgacgt ttggctacca cgtgtggcgg cgcatctgcc
    27301 cgctcgcggt tgcggggcaa atcggccgcc tcgtcggcaa accaggcgcg cgcaagatgg
    27361 gcgactggat gggcaagaac tacttgctcg tgcagctcgg ctcgatgatc gcagcgctgt
    27421 cgctgcgcct catcgtgacg aacggcagcg cgattggcgt ttcggtgttc ctcggcgtcg
    27481 tcgtcctcac cgcgatcgtc gtgtcgttcg tctacggcgg caagacgtgg tgcaacttca
    27541 tgtgccccgt cggtctcgtc gagaaaatct atacggagcc tgcgcgctcg gcggtgggtg
    27601 ccgcgccgga gaccgcgtcg caatgcacgc cgtgcgtcgc gtgcaagaag cactgccccg
    27661 atatcgatct ggagcaaggc tactggaagg aagttccgga acgcgacacg cgcggaaggc
    27721 ggctcgcgta cttcgcgtgg ccgggtatcg tcgtcgcgtt ctacacgtat tacttcctcg
    27781 cgcgcggcac gtgggactac tacttcgacg ggcgctgggc gtacgagcgc gacgtgctcg
    27841 ccgccgagcc gggcttctac ttctggcatg cgattccgcg cgcggtcgcc gcgccagtaa
    27901 cgctgatcgc gttcggcctt gcgagctacg tcgtcttcgc ctcgatcgag cgcatcgcgc
    27961 tgcggatccg aaaaccatcc gtagacgcga ccgaggagca gaaggagcag cacaccctgc
    28021 gcgtgcggca cgggatgctc gcgctatgcg gcctcatcgc attcaacgcg ttctactgct
    28081 tcgctggcca gccgacgctg cagaagctac ccgcgtgggt cgtcacgggg tggggcctca
    28141 tcgtcgtgtt cgcctcgacg acgatcttca tgcgccgcat ctcgcgacgc gaagaccaat
    28201 acgt
//
LOCUS       DATLBZ010000545         8607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5183964, whole genome shotgun sequence.
ACCESSION   DATLBZ010000545 DATLBZ010000000
VERSION     DATLBZ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5183964"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VMZ53_27365"
     CDS             <1..366
                     /locus_tag="VMZ53_27365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32262.1"
                     /translation="GSDNPESTNVTVTATSNNRFSGSAADVTADKYEDKDTYAFVTGA
                     ANELTVRLGWTGAANLDFFIFEAGSAAPVWRADGTTGTAETQTFSVKPSSNYWLLVGA
                     KGSSGLPAPYSATLCGGSY"
     gene            complement(360..1088)
                     /locus_tag="VMZ53_27370"
     CDS             complement(360..1088)
                     /locus_tag="VMZ53_27370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32263.1"
                     /translation="MRRFAACLLLAACGSPSDPATTPDAPATPDAAIDSASPSGFGEL
                     SGMCGVLGTPDLTGASPELIRATMTFARRYVDPDDRPLLTVGGQHLAETPNAGGSSGL
                     SEIFAYEELARCEHAALLKTETEIVYDTMGKITDLEVSIGQQKIGVSVTRAQTYPLGT
                     PYTESAATTLIMRKLNDIKASTANVSAADRWTKQVLAVLAWDTQAADTVAHVWSMLPA
                     DVKADTIVVVTTTNGDDTFIYTNQ"
     gene            1213..2220
                     /locus_tag="VMZ53_27375"
     CDS             1213..2220
                     /locus_tag="VMZ53_27375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32264.1"
                     /translation="MRWLSVAVLAVAACGRFGFDGVGPGDTGGAGDSGGGDDTLPGTD
                     GGDPDSSSTMQPDGAMAGNWAVFSPGLPALVDYYSAWAFGPSSVWVGGAAGDVRVFDG
                     SAWIQRPGAANDVYGMWGATASDVWEVGRLCEVHRWNGTMWNVVTVPGCTNKSYFGVA
                     GLSATDVWLVGVGGQVDHYVSGTFTAYPQANNVDLWAVWPKTASDAYFVGTRGTILHW
                     TGTVADESIGQNIILTSVWGSGNELWVVGGAGLIFHKVGTGAWQSVTSPTTTFLYHVF
                     GTSTSDVWAVGDSGVVLHYNGSTWSQVTSPVTTTLRWTAAVPGGGLRMVGHGGTVLAH
                     P"
     gene            2262..2690
                     /locus_tag="VMZ53_27380"
     CDS             2262..2690
                     /locus_tag="VMZ53_27380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32265.1"
                     /translation="MKAAATLAIVLLSACLMPPAQSGTGPSYASSSSSAPSEGGSDPT
                     MASSTPASAPAPAVAGPVSVTIRSACSKTVKVFYGEKPKFGSGTYSTISSNSVQSHSF
                     RQGDLFWIVDDSENGISSTSIGSSTRELEIGSSCTAISAR"
     gene            complement(2691..3356)
                     /locus_tag="VMZ53_27385"
     CDS             complement(2691..3356)
                     /locus_tag="VMZ53_27385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32266.1"
                     /translation="MMTKLRFLALALALVACKGKKQDTTPKNQGGDTGAKMDATLCES
                     SGKREATYDLNKDGKPDIWRLFKDNTLTCKQIDFDHDGNKDWLVAYDPSGKILYQKAD
                     FDFDGKFDMAAVFEPASGHRQEVERDTDFDGKFDIKEVYGADGSLSSVRRDRNGDNKP
                     DQWDQYLDGVLTATTYDDNFDGKVDRREDAPAGGQPKAPTPLEPTPAPAAGAGSGSAS
                     ASK"
     gene            complement(3372..3923)
                     /locus_tag="VMZ53_27390"
     CDS             complement(3372..3923)
                     /locus_tag="VMZ53_27390"
                     /inference="COORDINATES: protein motif:HMM:NF018924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HUS32267.1"
                     /translation="MRTIWVTAFLIALTACTTTMRISRDELQADLSKRFPKDIDKQIV
                     RLQLSEPQIEFFPGRPDILGVRVHVDITTESGNSHVAGTANVEGALEYVEAEHAFYLR
                     AARVKVLTLERPDGPGRAARLLDHASDAALKHAARIAIEEVLERHAIYRLDAKRSPRE
                     AKAIRHLREAHIDGHDLVLRVGM"
     gene            4016..5290
                     /locus_tag="VMZ53_27395"
     CDS             4016..5290
                     /locus_tag="VMZ53_27395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS32268.1"
                     /translation="MRGRTEVAVIGGGLAGLMAARVLAEQGVDVELLEAKPHVGGRVL
                     TMHPDAELPAELGPEFVHGEPDITLSLLREIRAERETVEETHHYRRGGKLVEEPDLWP
                     RFAKLLKKAPPASHDESARDYMQRVRMSSDEARLFAMLVEGFYASHLERISIASVAAD
                     VPEGGTGQARLIGGYGQLPEWLLARILRKHGSVHCGHVVHAIDWSSNIQIAFTGPGGR
                     QGMLVADRVIVTLPLAVLQQNAVSFHPQLGDHERAMRHLEMGHVVKVVACFNRRAWPE
                     QLRFVHSDGGKFPTFWMRSRGDHHQLTAWAGGPHAEALSRRTAGQLTTLAVTELARAI
                     DASATTIAGAVDHFHFHDYAADPYARGAYSYTKVGGAGAAEQLARPLGDRLFFAGEAT
                     DAEYEGSVAGALASGVRAAQQILATRSATRAA"
     gene            5309..6241
                     /locus_tag="VMZ53_27400"
     CDS             5309..6241
                     /locus_tag="VMZ53_27400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32269.1"
                     /translation="MRSWLFASCTVLIGCGSVSEQPKDAAVDSAVDVPRDVAVDTPAP
                     RCNPTAPFGSGTPVAELNSPQHDNFVWLTSDELTAYLSSQRTGGLGGYDIYVATRTSK
                     TAAFGTPQLLSTANTAMDESRPSLSSDGLTLYAAQAQSNGQFKIVSATRTSTTAAFGA
                     FSLVVDLNTTSVDIYDADPELAANGTAYFSSTRGGNADLYAATKNGTGFNGPLPVSAS
                     DIASPSFDGCVVISPDELTIFFCSNRTGGAGAFDMYMSQRSSVAVAFPAVTRLATLSS
                     ASNEQPSWISADGCLLYYSTGTTQNDIYVAQRGM"
     gene            complement(6242..7858)
                     /locus_tag="VMZ53_27405"
     CDS             complement(6242..7858)
                     /locus_tag="VMZ53_27405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS32270.1"
                     /translation="MNDKINILLVDDEPRNLDALEAILSDQDYHLIRAEDADRALRLL
                     LDNDDVAAIILDIQMPRVNGIELAQIIKGTKRFKQIPILFLTAHMVDDQDIVVGYGAG
                     AVDYLTKPFNPQILKAKIAVFADLFRKTRMLAELNDTLEARVSERTAELEKSEALLRA
                     AARQKDEFLAVLAHELRNPLAPLRTGLDLLIQMQAPTTPVVGRTLAAMNRQLDHIVRL
                     IDDLLDVSRISRGVLELKKQRVDVATLIHQTSETFRSLLEQRKISVSLDLPRAISVVV
                     DPTRISQIIGNLLHNASKFTPENGHVTVELKYDAGNALITVSDTGVGIPSDQLDRVFE
                     MFAAIERKAQTSRGAGIGLALAKRLAQMHGGNLIASSDGEGKGTTFTLSIPNAELARA
                     RTENTPAKGITSGPMGAAALGDPLQILVIEDNDDVADTLAEWLGHMGHIVKVARTGPS
                     GLELVLSTKPDVVLCDVGLPEMDGVEVCKQIRAQTGDFRPLVVALTGWGKEEDRKRTT
                     DAGFDHHLVKPVALDMLSDVLKRVGPRRTN"
     gene            complement(7855..>8607)
                     /locus_tag="VMZ53_27410"
     CDS             complement(7855..>8607)
                     /locus_tag="VMZ53_27410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS32271.1"
                     /translation="ELLGSPDIEIEAVGTGAEALAALREKRFDCCVVDLRLPDMTGFE
                     LLEQLQNESELRHVPVVVFTGKELSSEEQQRLKQVAKSVVLKDVQSPERLLDETSLFL
                     HRVIADLPEEKRRMIERLHGSTEALRGRKVLVVDDDARNIFALTTILENQEMEVISAT
                     NGRQAIEIIKSTPDLSVVLMDIMMPEMDGYETMREIRKDADFRTLPILALTAKAMKGD
                     REKCLEAGASDYIAKPVNTDQLLSLLRVWLYR"
BASE COUNT         1449 a         2789 c         2795 g         1574 t
ORIGIN      
        1 ggaagtgaca acccggagtc gaccaacgtc acggtgaccg ccacgtcgaa caaccgcttc
       61 agcgggtcgg ccgccgacgt gaccgcggac aagtacgagg acaaggacac gtatgcgttc
      121 gtgacgggcg cagcgaacga gctgaccgtg cgccttggct ggaccggtgc cgcgaacctc
      181 gacttcttca ttttcgaggc tggcagcgcc gcccccgtgt ggcgagccga cggcacgacg
      241 ggaaccgccg agacgcaaac gttctcagtg aagccgagca gcaactactg gcttctcgtc
      301 ggtgcgaaag gcagctccgg cctccccgcg ccgtattccg cgacgctctg cggcggcagc
      361 tactgattcg tgtagatgaa cgtgtcgtcg ccgttcgtgg tggtgacgac gacgatcgtg
      421 tccgccttca cgtcggcggg cagcatgctc cacacgtgag cgaccgtatc cgcggcctgc
      481 gtatcccacg ccagcacggc gagcacttgc ttcgtccagc gatccgcggc ggagacgttc
      541 gccgtcgacg ctttgatgtc gttgagcttg cgcatgatca gcgtcgtcgc cgcggattcc
      601 gtgtacggcg tgccgagcgg gtacgtctgc gcacgcgtca cgctgacgcc gatcttctgc
      661 tggccgatcg agacttcgag gtccgtgatc ttgcccatcg tgtcgtagac gatttcggtc
      721 tcggtcttga gcagcgcggc gtgctcgcac cgagcgagct cctcatacgc aaagatctcg
      781 gacaaccccg agctcccgcc tgcgttcggc gtctccgcga gatgctggcc accgacggtg
      841 agcagcggac gatcatccgg atcgacatag cgccgcgcaa acgtcatcgt ggcgcgaatc
      901 agctccggcg atgcgccggt gagatccggc gtcccgagca cgccgcacat tcccgacagc
      961 tcgccgaagc ccgacgggct cgcggaatcg atcgcggcat ccggcgtcgc aggtgcatcc
     1021 ggcgtcgtcg ctggatcgct cggcgatccg cacgcagcga ggagcagaca ggcagcgaag
     1081 cgacgcacgg cgcggaccct aacggaacag cgccacggcg caaagcgaat tctcccgacg
     1141 agtgcgagct tcccggacac ccgactccta ggatgtcctc aatcagacgt cgcaaggcgg
     1201 tacgatggca gtatgcgttg gttgtcggtc gccgtgctcg ccgttgcggc atgcggccgc
     1261 tttggcttcg acggcgtcgg ccccggcgac actggcggtg ccggcgacag cggaggtggc
     1321 gacgacacgc tcccaggcac cgacggtggc gacccggatt cctcgtcgac gatgcaaccc
     1381 gatggcgcga tggcagggaa ctgggcggtg ttctcgccgg gacttcccgc tctcgtcgac
     1441 tactactccg cgtgggcgtt cgggccgagt agcgtgtggg tcggcggcgc cgccggtgat
     1501 gtccgcgtgt tcgatggctc ggcgtggatc cagcggcccg gcgcggccaa cgacgtctac
     1561 ggcatgtggg gcgccaccgc gagcgacgtc tgggaagtcg gccggctgtg cgaagtccat
     1621 cgctggaacg gcaccatgtg gaatgtcgtc acggtgcctg gctgcacgaa caagagttac
     1681 ttcggggtcg cgggcctctc ggcgacggat gtctggctcg tcggcgtcgg cggccaagtc
     1741 gatcactacg tgtccggaac cttcaccgca tatccgcaag cgaacaacgt cgatctctgg
     1801 gccgtgtggc caaagaccgc gagcgacgcg tacttcgtcg gaacgcgcgg aacgatcctg
     1861 cattggaccg gcacggtcgc cgacgagagc atcggccaga acatcatcct gacgtcggtg
     1921 tggggcagcg gcaacgagct gtgggtcgtc ggtggtgcgg gcctgatctt tcacaaggtc
     1981 ggcaccggcg cgtggcaatc ggtgacgagc ccgacgacca cgttcctcta tcacgtgttc
     2041 ggcacgagca cgtcagacgt ctgggccgtc ggcgattccg gcgtcgtgct tcactacaac
     2101 ggctcgacgt ggtcgcaggt cacgtcgccg gtgacgacga cactccgttg gaccgcagcg
     2161 gtgcccggtg gcggcctgcg catggtcggt cacggcggca cggtcctcgc ccacccctga
     2221 gatttcgtgc gactgcctct ccgctcgcgg ctcttctcac catgaaagct gcagcaaccc
     2281 tcgcaatcgt ccttctttcc gcgtgcttga tgccgccggc gcagtccggc accggacctt
     2341 cctacgcgtc gtcgtcgtca tcggcgccgt ccgagggcgg cagcgatccg acgatggcga
     2401 gctcgacgcc ggcctctgcg cctgcgcccg ccgttgcagg ccccgtatcc gtcacgatcc
     2461 gctcggcgtg ctcgaagacg gtcaaggtct tctacggcga gaagccgaag ttcggcagcg
     2521 gcacgtactc gacgatcagc tcgaacagcg tgcagtcgca ctcgttccgc caaggcgatc
     2581 tgttctggat cgtcgacgac tcggagaatg gaatctcgtc gacgtcgatc ggatcgtcga
     2641 cgcgcgagct cgaaatcggc tcgagctgca cggcgatcag cgcgcgctaa ctacttcgat
     2701 gcgctcgcgg agccgctgcc cgcgccggcc gccggagcag gtgtcggttc gagcggcgtc
     2761 ggagctttcg gctgcccacc cgcgggtgcg tcttcacggc gatcgacctt gccgtcgaag
     2821 ttgtcgtcgt acgtcgtcgc ggtgagcacg ccgtccagat attggtccca ctgatcgggc
     2881 ttgttgtcgc cgttgcgatc gcggcgaacg ctcgagagcg agccatccgc gccgtacacc
     2941 tctttgatgt cgaacttgcc gtcgaagtcc gtgtcgcgct cgacttcctg gcgatgcccg
     3001 ctcgcgggct cgaacaccgc ggccatgtcg aacttgccgt cgaagtcgaa gtcggccttc
     3061 tgatacagaa tcttgccgga cggatcgtac gcgacgagcc aatccttgtt gccgtcgtga
     3121 tcgaagtcga tctgcttaca cgtcagcgtg ttgtccttga acaggcgcca gatgtcgggc
     3181 ttgccgtcct tgttcagatc gtacgtcgct tcgcgcttgc ccgacgattc gcacagcgtc
     3241 gcatccatct tggcgccggt gtctccgccc tggttcttcg gcgtcgtgtc ttgcttcttg
     3301 cccttgcacg ccaccagcgc cagcgcgagc gcgaggaatc ggagcttcgt catcacgggc
     3361 ccgaacttac actacatgcc cacgcgcaac acgaggtcgt gaccgtcgat gtgggcttcg
     3421 cgtaggtgcc gaatcgcctt ggcctcacgc gggcttcgct tcgcatcgag gcgataaatc
     3481 gcatgccgct ccagcacttc ttcgatggcg atacgcgccg catgcttcag agccgcgtca
     3541 ctcgcgtggt cgaggagtcg cgcggctcgt cccggaccgt cggggcgctc cagcgtgaga
     3601 actttcacgc gcgcggcgcg caggtaaaat gcatgctcgg cctcgacgta ctccagcgcg
     3661 ccttcgacgt tcgcggtgcc ggcaacgtgt gaattgccag actcggtcgt gatgtcgacg
     3721 tgaacgcgta cgccgaggat atcgggacgg cctggaaaga attcgatctg gggctcgctt
     3781 agctgcagcc gaacgatttg cttgtcgatg tccttcggaa agcgcttcga gagatccgcc
     3841 tgtagctcgt ctcgtgagat ccgcatcgtg gtcgtgcacg cggttagcgc gatcagaaat
     3901 gccgtgaccc aaatcgtccg catgatgaaa agagccgcca tacgaacgtg aggttgacgg
     3961 cacgaggcgg caaatctcgc agcgacgccg cacgtgtttt gcagcttatc gatgtatgcg
     4021 cggtcgaacg gaagttgcag tcatcggagg tgggctcgca gggctcatgg cggcacgcgt
     4081 gctcgccgag cagggcgtcg acgtcgagct gctcgaggcc aaaccgcacg tcggtgggcg
     4141 cgtgctcacg atgcacccgg atgcagagct gcccgcggag ctcggtcccg agttcgtgca
     4201 tggcgagccg gacatcacgc tgtcactgct gcgggaaatt cgcgcagagc gcgagaccgt
     4261 cgaggaaacg catcactatc gccgcggcgg caagctcgtc gaggagcccg atctctggcc
     4321 gcggttcgcg aagctcttga agaaagcacc gcctgcttcg cacgacgagt cagcgcgcga
     4381 ctacatgcag cgcgtgcgca tgagcagcga cgaggcacgt ctcttcgcga tgctcgtcga
     4441 gggcttctac gcgtctcacc tcgagcgcat cagcatcgcg agcgtcgcgg cagacgtgcc
     4501 cgaaggcggt accgggcaag cgcggctgat cggcggctat ggccagctgc ccgagtggtt
     4561 gctcgcgcgc atcctccgca agcatggctc cgtccactgc ggtcacgtcg tgcacgcgat
     4621 cgattggagc tcgaacatcc agatcgcgtt cacgggaccc ggcggccgcc aaggcatgct
     4681 cgtggcagat cgcgtgatcg tcacgctgcc actcgcggtg ctgcagcaaa acgcggtgag
     4741 ctttcatccg cagctcggtg atcacgagcg agcgatgcgc cacctcgaga tgggccacgt
     4801 cgtgaaagtc gtcgcgtgct tcaaccggcg cgcgtggcca gagcagctgc gcttcgtgca
     4861 cagcgatggc gggaagttcc cgacgttctg gatgcgttcg cggggcgacc atcaccaact
     4921 cacggcgtgg gcaggtggcc ctcatgccga ggcgctgtcg cggcgaactg cgggacagct
     4981 cacgacgctc gcggtgaccg agctcgcaag agcgatcgat gcgagcgcga cgacgatcgc
     5041 cggcgccgtc gaccacttcc actttcacga ctacgcggcg gacccgtacg cgcgcggtgc
     5101 ctacagctac acgaaggtgg gcggagccgg cgcagcggag caactcgcgc gaccactcgg
     5161 cgatcgcctg ttcttcgccg gcgaagcgac cgacgccgag tacgaaggtt ccgtcgcagg
     5221 cgcacttgcg agcggcgtac gtgcagctca gcagatcctt gccacgcgat ctgcaacgcg
     5281 cgcggcgtag tgtgtatctt cgtcggcgat gcgtagttgg ttgtttgcgt cgtgtacggt
     5341 gttgatcggt tgcggcagcg tctcggagca gcccaaagat gcggcggttg attcggcggt
     5401 cgacgtcccg cgcgacgtcg ccgtcgacac cccggcaccg cgctgcaacc caacggcacc
     5461 attcggatcg ggcacacccg ttgccgagct caactcgcca cagcacgaca acttcgtgtg
     5521 gctcacgagc gatgagctca ccgcgtatct ctcgagccag cgcactggcg ggctcggcgg
     5581 ctacgacatc tatgtggcga cgcggacgtc gaagaccgcg gcattcggca cgcctcagct
     5641 gctgagcacc gcgaacacgg cgatggacga gagcaggccg tcgctctcct cggacgggct
     5701 cacgctgtac gcggcgcagg cgcagtcgaa cggccagttc aagatcgtct ccgcgacccg
     5761 cacgtcgacg acggccgcat tcggagcgtt ctcgttggtc gtcgacctga acacgacatc
     5821 tgtcgacatc tacgacgctg accccgagct cgcagcgaac ggcaccgcct acttctcatc
     5881 gacgcgcggc ggtaatgcgg atctgtatgc tgcgaccaag aacggcaccg gcttcaacgg
     5941 ccctctcccg gtcagcgcct cggatatcgc gagcccgtcg tttgacggct gcgttgtcat
     6001 ttcgccggac gagctgacga tcttcttctg ctcgaatcgc acgggcggcg caggcgcctt
     6061 cgacatgtac atgtcgcagc gctcgtcggt ggcggtcgcg ttcccggcag tgacgcgact
     6121 tgccacgctg tcatcggcga gcaacgaaca accgagctgg atctcggccg acggctgcct
     6181 gctctactac tcgaccggca cgacgcagaa cgatatctac gtcgcgcagc gcggcatgta
     6241 gctagttcgt gcgacgtggt ccgacgcgct tcaaaacgtc ggacagcatg tcgagcgcga
     6301 cgggcttcac gaggtgatga tcgaagcccg catcggtggt gcgcttgcga tcttcttcct
     6361 tgccccagcc ggtgagcgcg acgacgagcg gacggaagtc gccggtctgc gcgcggatct
     6421 gcttgcagac ttcgactcca tccatctcgg gaaggccaac gtcgcagagc acgacgtcag
     6481 gcttcgtcga gagcacgagc tcgaggccac ttggccccgt gcgcgcgacc ttcacgatgt
     6541 gccccatgtg gccgagccac tccgcgagcg tatcggcgac gtcgtcgttg tcctcgatga
     6601 cgaggatctg cagcggatcg ccgagcgccg ctgcgcccat cgggcccgac gtgatgccct
     6661 ttgccggcgt gttctccgta cgcgcgcggg caagctctgc gtttggaatc gacaacgtga
     6721 acgtcgtgcc cttgccctcg ccgtcgctcg acgcgatcag gttaccgccg tgcatttgtg
     6781 cgaggcgctt cgcgagcgcg agcccgatgc ccgcaccacg cgacgtctgc gccttgcgct
     6841 cgatcgccgc aaacatctcg aacacgcgat cgagctgatc ggacgggatg ccgacaccgg
     6901 tatcgctgac cgtgatcagc gcgttgccgg cgtcgtactt gagctcgacg gtgacgtgac
     6961 cgttctccgg tgtgaacttc gacgcgttgt gcagcaagtt gccgatgatc tgcgagatgc
     7021 gcgtgggatc gacgacgacc gagatcgcac gcggcagatc gagcgacacc gagatcttgc
     7081 gctgctcgag cagcgaccgg aatgtctccg acgtctgatg gatcagcgtt gccacatcga
     7141 cgcgctgctt cttgagctcg agcacgccgc ggctgatgcg cgacacatcg agcagatcgt
     7201 caatgagccg cacgatgtga tcgagctggc ggttcatggc agcgagcgtg cgcccgacga
     7261 ccggcgtggt cggcgcttgc atctggatga gtaggtcgag gcccgtgcgc agcggcgcca
     7321 aggggttgcg cagctcgtgc gcgagcacgg cgaggaactc gtccttctgg cgagccgctg
     7381 cgcgcagcag ggcttccgat ttctcgagct ctgccgtgcg ctcgctgacg cgcgcttcga
     7441 gcgtgtcatt gagctcggcc agcatgcgtg tcttgcggaa cagatccgcg aacacggcaa
     7501 tcttcgcctt gaggatctgc ggattgaacg gcttggtcag gtagtcgacg gcgccggcgc
     7561 cgtaaccgac gacgatgtcc tgatcgtcga ccatgtgagc agtcaggaac agaattggaa
     7621 tctgcttgaa ccgcttcgtg cctttgatga tctgcgccag ctcgatgccg ttcacccgcg
     7681 gcatctggat atcgagaatg atcgctgcga cgtcatcatt atcgagcagc aggcgcagcg
     7741 cgcggtcagc gtcctcggcg cgaatcaggt ggtagtcctg atccgacagg atcgcctcga
     7801 gggcatcgag gtttcgcggc tcgtcatcga cgaggaggat gttgatcttg tcgttcatcg
     7861 atacaaccag actcggagca gcgagagcag ctgatccgtg ttgacgggtt tggcaatgta
     7921 gtcagaggca ccagcttcga gacacttctc gcgatcaccc ttcattgcct tggcggtgag
     7981 agcgaggatc ggcagcgtac ggaagtcagc atccttgcgg atttcgcgca tcgtttcgta
     8041 gccgtccatt tcgggcatca tgatatccat gagcacgacg ctgaggtcgg gcgtcgactt
     8101 gatgatctcg atcgcctggc gaccgttcgt cgccgagatg acttccatct cctgattctc
     8161 gaggatcgtc gtgagcgcga agatgttgcg cgcgtcatcg tcgacgacga gcaccttgcg
     8221 gccacgcagc gcctcggtcg agccatgcag gcgctcgatc atgcggcgct tttcctcggg
     8281 caggtcggcg atcacgcgat gcaagaacag cgacgtctcg tcgagcaagc gctcgggcga
     8341 ctgcacgtcc ttgagcacga cgctcttcgc gacttgcttg agccgctgct gctcctccga
     8401 cgagagctcc ttgccggtga acacgacaac ggggacgtgg cggagctccg actcgttctg
     8461 cagctgctcg agcagctcga accccgtcat gtccggcagg cgaagatcga cgacgcagca
     8521 atcgaaccgc ttctcgcgca gcgctgcgag cgcctccgca ccggtgccaa cagcctcgat
     8581 ctcgatgtcc ggcgacccaa gcagctc
//
LOCUS       DATLBZ010000546         5084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_718963, whole genome shotgun sequence.
ACCESSION   DATLBZ010000546 DATLBZ010000000
VERSION     DATLBZ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718963"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VMZ53_27415"
     CDS             <1..519
                     /locus_tag="VMZ53_27415"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUS32272.1"
                     /translation="VLTGGPDAISGSYIQPTVLTNVDHKMKVMSEETFGPVIPIQKVK
                     SVDEAVQLANDSKYGLGSAVFAGKAARAIADRLRAGMTSINSVQAFAGIPTLPFGGIG
                     ESGFGRIHGEEGIREFTRIKSTAEQAFSLPINMMSFRQPKNAIGNIKGMIKQLHGTGI
                     VAKAGDFLRKLK"
     gene            532..1077
                     /locus_tag="VMZ53_27420"
     CDS             532..1077
                     /locus_tag="VMZ53_27420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HUS32273.1"
                     /translation="MRWWLVALLLGGCDSLFNLDHLREPARLDASGTGDGESQPDAPA
                     DAENTNAMCAPSYNGQRYQVKSLPYTWELADNYCKSLQVAGSSKYSHLVVITDPAEAA
                     VVGALISASETAWVGLTYVPSQWAWVTTEPTGAIPWATGQPDQIGTQNCGRIIPDVGL
                     DNETCSMSYQFICECDAYPPE"
     gene            complement(1078..1965)
                     /locus_tag="VMZ53_27425"
     CDS             complement(1078..1965)
                     /locus_tag="VMZ53_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HUS32274.1"
                     /translation="MTLPTVEQVRTVAVIGAGTMGHGIAHVVAMMGAEVRLYDALAGA
                     ARGGIDKIGKNLAKGVELGKVTAADRDATLGRLQACDELAAACTGADCVIEAIPERID
                     LKREIFAAIDASAPKHALFATNTSSLPISEIASAVADGTRLVGMHFFNPVHVMKLVEV
                     VRHKRSSDAAISLAVALAEKMGKTPITVTDSPGFASSRLGLVIGLEAMRMVEQGVASA
                     ADIDTAMKLGYGHPMGPLELTDLVGLDVRLGIAEYLTEAIGKNFEPPRVLRDKVAEGK
                     LGKKSGEGFYRWSPDGKRL"
     gene            1997..3646
                     /locus_tag="VMZ53_27430"
     CDS             1997..3646
                     /locus_tag="VMZ53_27430"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUS32275.1"
                     /translation="MQRSLNAAETDRLQRELESIVGPRRVSMRTVDLDTYARDMWPRM
                     LIAYRDGILPAHRPHAVVWPETVREVVSIVRLAREIKLPIIPYGGGSGVCGGAIPLYG
                     GITIDTKRMQQLRSVRGDELVCDVEAGLSGERFERELARRGYTFGHFPSSIYCSTVGG
                     WVATRAAGQLSTKYGKVEDRVAGLTVVTGKGEVIETDGPNRALRGPNWTQLVVGSEGT
                     LGIITSARLRVSPAPQLRVFRGFEVDDVAAGVEAIRRVLQRGLRPAVIRLYDELDTLM
                     NSFGHHGEEPVEDTVHGPLPPISTGALPAYPAPQPPKQDGVVDRLLAIATGKGLRGSA
                     MKTALGHSEIVNKLFGTVAEKIARRGCRLIIGLEGARIRTEVEAALTFAELEALGARD
                     KGEDPGWAWLRHRYAVSYRMSPVFRDGAFVDTMEVAASWERLMDLYHSVRTAIGRHAV
                     VMAHFSHAYADGCSIYFTFAGHAPDAQAASRLYDSIWRDGLEATTRVGGTISHHHGVG
                     LLKAPYMAGEHREAMALFEAAKQALDPDGVFNPGKMGLRTR"
     gene            3643..4896
                     /locus_tag="VMZ53_27435"
     CDS             3643..4896
                     /locus_tag="VMZ53_27435"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUS32276.1"
                     /translation="MTLQAELEAAVGAPNVELLDFGDLPSFRVAPCSAAEVAEVIRRA
                     ASLGAAIHPVGTGGRPSRVDKDPRGERSRVFISTSRLDQVLQLDEHSLLVHAQAGLTG
                     IDLERILAPRGLSIGDYPPVVLASSLGGILAVRTPGKSSARHGFFEDAVVGVSAVLAD
                     GRTIHTRVAPRRATGPDLSRALCGSEGTIGFITSAVVRIHPKPESRLVAAYLLPSFDA
                     AISAVLFALREEAAPSGVRIYDAAEAARHFDGLSLPAGNVLLCAATAGPTDLAACDRD
                     LITSAVNAEGGAQTDHGLADLWWRRLHAGEPTPGPAPTLQVMATPSKLRPVYAAVNAD
                     AKRLGAQARAHVSRFDADGAVMFFTFEKDGGPADDTTCAALEKSAEQAGAWLLGARAT
                     KLDSYLRALRDTLDPQGIMNPGTLS"
BASE COUNT          849 a         1701 c         1677 g          857 t
ORIGIN      
        1 gtcctgaccg gcggtcccga cgcgatcagc ggcagctata tccagccgac cgtgctcacg
       61 aacgtcgatc acaagatgaa ggtgatgagc gaggagacgt ttggaccggt catcccgatc
      121 cagaaggtga agtcggtcga cgaagcggtg cagctggcga acgattcgaa gtacgggctc
      181 ggctctgccg tgttcgccgg caaggcagca cgcgcgatcg cagatcgcct gcgcgccggc
      241 atgacgtcga tcaactcggt gcaagcattc gcgggcattc cgacgctgcc gttcggcggc
      301 atcggcgagt cgggcttcgg ccgcatccac ggcgaagaag gcatccgcga gttcacgcgc
      361 atcaagtcga cggccgagca ggcgttctcg ctgccgatca acatgatgtc gttccgccag
      421 ccgaagaacg cgatcggcaa catcaagggc atgatcaagc agctgcacgg cacgggcatc
      481 gtggcgaagg ccggcgattt cctccgcaag ctgaagtagt acaatcacag catgaggtgg
      541 tggctcgtcg ctctgctgct aggcggttgc gacagcctgt tcaacctcga tcacctccgc
      601 gagcctgcgc gcctcgacgc gagcggaacc ggcgacggcg agagccagcc cgacgcaccc
      661 gctgatgcag aaaatacaaa cgcgatgtgc gcaccgagct acaacggcca gcgctatcag
      721 gtgaagagcc tcccgtacac gtgggagctt gccgacaact actgcaagag cttgcaggtt
      781 gcgggcagct cgaagtactc gcacctcgtc gtgatcacgg atccggccga ggccgccgtc
      841 gtcggtgcgt tgatctcggc gtccgagacg gcatgggtcg ggctgaccta cgtgcccagc
      901 caatgggcat gggtgaccac ggagccgacg ggcgcgatcc cgtgggcgac cggacaacct
      961 gatcagatcg ggacgcagaa ctgcggtcgc atcatcccgg atgtcggtct cgacaacgag
     1021 acgtgcagca tgagctatca gttcatctgc gagtgcgacg cgtacccgcc cgagtgacta
     1081 gaggcgcttg ccgtcgggcg accagcgata gaagccttcg ccgctcttct tgccgagctt
     1141 gccttcggcg accttgtcgc gcagcacgcg cggcggctcg aagttcttgc cgatcgcctc
     1201 ggtgaggtac tcggcaatgc cgagccggac gtcgagcccg acgagatccg tgagctcgag
     1261 cggccccatc ggatgaccgt agccgagctt catcgcggta tcgatgtcgg ccgccgacgc
     1321 gacgccctgc tcgaccatgc gcatcgcttc gagcccgatc acgaggccca ggcggctcga
     1381 tgcaaagccg gggctgtctg tcaccgtgat cggcgtcttg cccatcttct cggcgagcgc
     1441 gacggcgagc gaaatcgcgg catcgctcga gcgtttgtgg cgcacgacct cgacgagctt
     1501 catcacgtgc acgggattga agaagtgcat accgacgagg cgcgttccat ctgccactgc
     1561 gctcgcgatc tccgaaatcg gcaacgacga cgtgttcgtc gcgaacagcg cgtgcttggg
     1621 tgccgacgca tcgatcgccg cgaagatctc gcgcttcaga tcgatccgct cggggatcgc
     1681 ttcgatcacg cagtcggcgc cggtgcacgc agcggcgagc tcgtcgcacg cttgcagacg
     1741 accgagtgtt gcgtcgcggt cggcggcggt caccttgccg agctcgacgc ccttggcgag
     1801 gttcttcccg atcttgtcga tgccgccgcg tgcagcacct gcgagtgcgt cgtacagacg
     1861 aacctcggca cccatcatgg cgaccacgtg ggcgatgccg tggcccatcg tgccagcgcc
     1921 gatcacagcg acggtacgga cttgttcgac ggtcggaagc gtcacgcgtg gaacatacct
     1981 gatataacgc gcgtcggtgc aacgctcgct gaacgctgcc gagaccgatc gactgcaacg
     2041 cgagctcgag tcgatcgtcg gaccaaggcg cgtctcgatg cgcacggtcg atctcgatac
     2101 gtacgcgcgc gacatgtggc cgcgcatgct gatcgcgtat cgcgacggca ttctgcctgc
     2161 gcatcgcccg cacgcggtcg tgtggccgga gaccgtgcgc gaggtcgttt cgatcgtgcg
     2221 gctcgcccgc gagatcaagc ttccgatcat tccgtacggc ggtggctcgg gcgtctgcgg
     2281 cggcgcgatt ccgctgtacg gcgggatcac gatcgacacg aagcgcatgc agcagctgcg
     2341 ctcggtgcga ggcgacgagc tcgtctgcga tgtcgaggcg ggcctctccg gtgagcgatt
     2401 cgagcgcgag ctcgcgaggc gcggctacac gtttggtcac ttcccgtcgt cgatctattg
     2461 ctcgacagtc ggcggctggg tcgccacgcg cgccgcgggc cagctctcga cgaagtacgg
     2521 caaggtcgaa gatcgcgtcg ctggtctcac cgtcgtcacc ggcaagggcg aggtgatcga
     2581 gaccgatggt ccgaaccgcg cgctgcgcgg ccccaattgg acgcagctcg tcgtcggcag
     2641 cgaaggcacg cttggcatca tcacgtcggc gcgccttcgg gtgtcgcccg cgccgcagct
     2701 acgcgtgttc cgcggcttcg aggtcgatga tgtcgcagcg ggcgtcgaag cgattcggcg
     2761 cgtgctgcaa cgcggccttc gccccgccgt gatccgcctc tacgacgagc tcgacacgct
     2821 gatgaactcg ttcgggcatc acggcgagga gcccgtcgag gacaccgtgc acggcccgct
     2881 tccgccgatc tcgacaggcg cactgcccgc ctaccctgcg ccgcaaccac cgaagcaaga
     2941 cggcgtggtc gatcgactgc tcgcgatcgc gacgggcaag ggcttgcgcg gcagcgcgat
     3001 gaagaccgcg ctcggccaca gcgagatcgt gaacaagctg ttcggcaccg tcgccgagaa
     3061 gatcgcgcgg cgcggctgcc gcttgatcat cggtctcgag ggcgcacgca ttcgcaccga
     3121 ggtcgaggcc gcgctcacgt tcgccgagct cgaagcactc ggtgcgcgcg acaaaggcga
     3181 ggatccggga tgggcgtggc tgcgtcaccg ctacgccgtc agctatcgga tgtcgcctgt
     3241 atttcgcgat ggcgccttcg tcgacacgat ggaggtcgcg gcgagctggg agcggctgat
     3301 ggacctctac cactcggtcc gcacggcgat cggccggcac gcggtcgtga tggcgcactt
     3361 ctcccacgcg tacgccgatg gctgctcgat ctacttcacg ttcgcggggc acgcgcccga
     3421 cgcacaggct gcttcgcgcc tctacgattc gatctggcgc gatggcctcg aagcgacgac
     3481 gcgcgtgggc ggcacgatca gtcatcacca cggcgtcggc ctgctcaagg cgccgtacat
     3541 ggcgggcgag catcgcgagg cgatggcgct gttcgaagcg gcgaagcaag cgctcgatcc
     3601 cgacggcgtg ttcaacccgg gcaagatggg actgcgcacc cgatgacgct gcaagccgag
     3661 ctggaagctg cggtcggcgc gccgaacgtc gagctgctcg atttcggtga tctgccatcg
     3721 ttccgcgtgg cgccctgctc cgccgcagaa gtcgccgagg tgatccggcg agccgcgtcg
     3781 ctcggcgctg cgattcatcc cgtcggcacg ggtggccgac catcccgcgt cgacaaagat
     3841 ccgcgaggcg agcgctcgcg cgtgttcatc tcgacgtcgc gcctcgatca ggtgctccag
     3901 ctcgacgagc attcgctgct cgtgcacgca caggcagggc tgacgggcat cgatctcgag
     3961 cgcatcctcg cgccgcgagg tctttcgatc ggcgattacc caccggtcgt gctcgcgtcg
     4021 tcgctcggcg gcatcctcgc agtgcgcaca ccaggcaaat cgagtgcgcg gcacggcttc
     4081 ttcgaggacg cagtcgtcgg cgtctcggcg gtgctcgcgg atggccgcac gatccacacg
     4141 cgcgttgcgc cgcgtcgcgc gaccggtccc gatctctctc gcgcgctgtg cggcagcgaa
     4201 ggcacgatcg gcttcatcac gagcgcggtc gtgcgcattc atccaaagcc cgagtcgcgc
     4261 ctcgtcgcgg cgtatctgct gccatcgttc gacgccgcga tcagcgccgt gctgttcgcg
     4321 ctgcgcgagg aagctgcacc atccggcgtt cgcatctacg acgccgcgga agctgcccgc
     4381 cacttcgatg gcctgtcact gccggcaggc aacgtgctgc tctgcgccgc gaccgcggga
     4441 ccgacggatc tcgcggcgtg cgatcgcgac ctcatcacga gcgcggtgaa tgcggagggc
     4501 ggcgcgcaga ccgatcacgg gctcgctgat ctctggtggc gacgccttca cgctggcgag
     4561 ccgacgcccg gtcccgcacc gacgctgcaa gtgatggcga cgccgagcaa gctgcgcccc
     4621 gtctacgccg cggtgaacgc cgacgccaag cgacttggag cccaggcccg cgcgcacgtc
     4681 tcgcgcttcg acgccgatgg tgccgtcatg ttcttcacgt tcgagaagga cggcgggcct
     4741 gcggatgaca cgacatgcgc cgcgctcgag aagtcggccg agcaagcggg tgcgtggctg
     4801 ctcggcgcac gcgcgacgaa gctcgactcg tacctgcgcg ccctccgcga cacgctcgac
     4861 ccgcagggca tcatgaatcc gggcactctc agctaacgcg actcagatgc gtcctagatg
     4921 caactaaagt tgcattgatg caacgcagcc gggatctgca atcttgttgt tccgcgcggt
     4981 tactacgtgt aggtgttggc ccaccccttg taatacgcag cgtccatcat gcgtagcctc
     5041 aacaacatcg tcaccgtcct cgttctctcc ttcgcttcga tctc
//
LOCUS       DATLBZ010000547        20413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2204378, whole genome shotgun sequence.
ACCESSION   DATLBZ010000547 DATLBZ010000000
VERSION     DATLBZ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20413
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204378"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..694
                     /locus_tag="VMZ53_27440"
     CDS             188..694
                     /locus_tag="VMZ53_27440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32277.1"
                     /translation="MDAIATRLEQLATRAAVASVSTAAVSAVVVAAIAFVEQQLVVAG
                     VVEQQLVAAIVVIVVIELIVVAPVVIIEQQLVAAVIAVEQLAFVVKRRLALGAPVERR
                     RRALVFSLRWWRWTPALGASASGAWRERARVRYLRNSHLWRSRYITIATLTTSAITLP
                     TDGCLNSS"
     gene            complement(589..1110)
                     /locus_tag="VMZ53_27445"
     CDS             complement(589..1110)
                     /locus_tag="VMZ53_27445"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HUS32278.1"
                     /translation="MTSPLAHKVEEVAAHISETVKTEVERVDFGLRVIIVWKAIKAVL
                     LFALGIAAFALVHSDVAKLGTELIAWLGIDAGRPTVQHVLAKLAGLTPHRISVIGIGA
                     IIYACVLALEGWGLHRRYLWADWLTVIVTSSLIPLEIYELFKHPSVGKVIALVVNVAI
                     VMYLLRHKWLFRK"
     gene            1149..2768
                     /locus_tag="VMZ53_27450"
     CDS             1149..2768
                     /locus_tag="VMZ53_27450"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HUS32279.1"
                     /translation="MKRALVVLGLSFVGCSNPNPPHTERPVVMSGGATVTRVAPDTLP
                     VLDAWANAMGGRAAISSIRAVHAKGSYERGGMRGTIELWETASGQRREEIIQGPLREL
                     RVFDGTRGWLVDRNREVRELAAFELDDQMTLAFIGAHAALLTDRRAGDVKLDGDKVVL
                     APDGSRRPASVWLDRGTHLPDHYEKRDGERTRKITWSDWNPVGNTGAKLPWSQREENG
                     NPNDAVTIHWTKLEAATPPDAAFAQPADRDPDMSLASEPVTVPIEAVYGDPSVTKGGL
                     IFVKVSINDQPMSFILDTGAEATVLNSSRVSKLGLQGVGTFATGAGGGDVVLSYVPGV
                     TTKVGGATVSNQIVAAILLDDIEKMLGRPLDGILGYDFLSRFVIEIDYANQQMRLFDR
                     TKYHHTGSGKPIAITLEDSTPFFDASVEIPKRGLLAGHFVLDTGCLCDVQLFTPFVDA
                     NNLLAALPDAKQSGMSAGAGGQTKEVSASIPSIHIGDVVVKDVRADLARDTFGAMADP
                     ESTGLIGGLTFGRFTLVLDYKSKQVFLDPAK"
     gene            complement(2769..3176)
                     /locus_tag="VMZ53_27455"
     CDS             complement(2769..3176)
                     /locus_tag="VMZ53_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS32280.1"
                     /translation="MITKHTHTTIYVTDQDRAKAFYTEKLGFEVRDDASMGDFRWLTV
                     GPKTQPDVCLILYAIKPGPWMKEEHASSLRTLVEAGAIGGGVLMTDDCKRDYDELKAK
                     GVEFTREPKEMPYGLEAVFKDDSGNYYSLTQRR"
     gene            complement(3214..3615)
                     /locus_tag="VMZ53_27460"
     CDS             complement(3214..3615)
                     /locus_tag="VMZ53_27460"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HUS32281.1"
                     /translation="MASGALLRRLVRARDLLHADAARNPTLDELAAASGVSRAHLARN
                     FTQTFGISPHQYLVQLRLEQARRALAAGTSVTEVCHEVGFESLGTFSSTFRKRVGLSP
                     REYQRRARPFVQSLGVPILYIPSCFLYGAHV"
     gene            3655..5082
                     /locus_tag="VMZ53_27465"
     CDS             3655..5082
                     /locus_tag="VMZ53_27465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32282.1"
                     /translation="MVRIALILLALAACKREKAGPGASGGPGSGVVTAGSASTGSAAT
                     TAASGSAGSGSATVAGPIDLLHAIPVRMAVSSLVANPKVSAYDLVDGDLATAWNSKTG
                     QLKGAWIAFRVPPSTHVDAIELTVGFVSKGAEGDYFTLNPRIKSVAVWHDGIRLREVE
                     LDINKRELQKVPVDANGGDYKLEVTAFEPGSKKNWREICVSELRVIGTPAPGTKTGDY
                     SPSISIASLDAEPLPEPNVTLEVLDGFASVAEFCDAFKKRKPAPCAPWDEKCEKTPEP
                     STCGDAVGEPLTLPQLPSGWKATWFASYLGNSKVAACNLAIEAKGRFYVLERIGGNGA
                     IGSASCGQAIDMQLDLAKPTRSRSDIRVEDVMGTPDKELVLLTTVPGDIDPMDSNKLP
                     FTEQLHICSATPSGEPACMKAKLGQLDVKNASGDDGYAQWLEAAWLDDFAIEHGQLVI
                     TRKSGKAESYEQDLAPGRYRLKLPD"
     gene            5106..6422
                     /locus_tag="VMZ53_27470"
     CDS             5106..6422
                     /locus_tag="VMZ53_27470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32283.1"
                     /translation="MVRTAAMTMLALVACKRVGDGPAATGSGSAAPPVISEAGVEVAN
                     AGSAAGVAPSGSVDLLHAVPARVAVSSLVKNPAITPFHLVDGSLDTAWNSKTGELVGS
                     WVAFRVPAAAHITELQMTAGFVSSGDEGDYFTLNPRIKHVAIWRNGAKLADRDLDITV
                     RALQNIPIGNEGGDYKIEVTAIEPGSKKNWRETCISELRVMGSATGMRPNHSVPEMSI
                     GSLDAKPLEDSALYLVPPKDFGSIKDFCATLAADPKRTAKIVSCGDAAQAALELPSPL
                     PAAWSAHWFCAKTETPSPAKVNAGKMIKRSECFLAAEANNRFFILEVPGLFDPVVTIR
                     ESYMNVGENSRRSVELVDAAGSPDKDLIFVTTSGPTEWLTICAPTPTGAPACGSKQIA
                     EMEFHNNNSDYGFDEYYTYKWRRDYQLGDELDLGSDVPPVPIRILE"
     gene            6419..7051
                     /locus_tag="VMZ53_27475"
     CDS             6419..7051
                     /locus_tag="VMZ53_27475"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUS32284.1"
                     /translation="MKALLYTTAVAGVLAALCVLAIDQSFAKWISTRETYPDVWNTII
                     GYLEYPLGVEPNKWIGVYVLVAGAVLTLVVTPLRKYAHIWSLLALSHLLGRNMITWLK
                     PPLGRWRPTEWLKHHASSDTFFHKAAYSFPSGHVILFASMIFPLALAYPKARPLLGVV
                     LFAMVARVAVNAHFLGDVFGGLALSAIGTWACAVAVRRALPSQIRPPYLQ"
     gene            complement(6969..7868)
                     /locus_tag="VMZ53_27480"
     CDS             complement(6969..7868)
                     /locus_tag="VMZ53_27480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUS32285.1"
                     /translation="MKSRGVLYMTLSALGFSVMSVLVKLAAPRLPTGEIVLARALMTL
                     VLSYVMVKRAVGSDGKPLSPWGVQRGKLVFRGLLGFSALGCYYIALARLPLADATTLH
                     YIQPLITGVLAWWILDEKIGWAAAFAIACGVAGVVAVAHPGIGTVDDPVGSLVAVASA
                     TLSSIAYVTVRQLSRTEHPLVIVFYFPLVATPLAIPWAAANFVMPDARAWLLLIGIGI
                     ATQIGQVFLTMGLTIERAGRATSVGYIQICFALLWQLLLFEQTPALGTLAGAALIIGG
                     TVVVSATAKRDAPPPHTPTSRSR"
     gene            complement(7875..9347)
                     /locus_tag="VMZ53_27485"
     CDS             complement(7875..9347)
                     /locus_tag="VMZ53_27485"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HUS32286.1"
                     /translation="MNPTLEELLQPLRETATNAAKLVQHLGTRLDAMALDLARDGGAV
                     VRHAQALAGAARDEAVHARDQASSFTKATPRMTKIATVAAALFARQRWLRLAQAARTG
                     SPELRPEDHRDLAVRTAAYAAELRGGIAKIGQLASCRPDMIGPVWAAELAKLQDEVPP
                     VDAAAIRARIESELGRSIDELFVEFDDVPIAAASLAQVHAAVLRDKAAEGGRGERVVV
                     KVQVPGIEDVIAADIAALKTIASTVGEVPGVDLPTLATELARALAVELDYEAEADSLW
                     GYTGSAVVPRPHPDASSKRVLTMSRIEGEKLTSWIEKATPEARDRLLGELVGEVAAQI
                     LVRGQVHADPHPGNFLVTADGKLALLDFGCMLELSKPERAAYARLVLAIAGGNHAAAG
                     KELATLGFSADDPEQLVDLTSALIGAMKPGMNASELDWQAAFADQIAQAKQLGGLVIP
                     RSFVLLGRVLASVAGLMATYKPKVEIHPLIARHLAAAIAQ"
     gene            complement(9356..9778)
                     /locus_tag="VMZ53_27490"
     CDS             complement(9356..9778)
                     /locus_tag="VMZ53_27490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32287.1"
                     /translation="MTRKRPPTPAPDDTDEPEKKERVIHTRVPESLEAELRQRAQELG
                     MSVSNLVRNVLGHAFGLVGDVVADSHAIARAARGDARAKAHPTEPDEVIAWQPIVLAK
                     NAICSRCNDILPKGSDASISIGGDRAIVCAKCLAELRK"
     gene            9882..10286
                     /locus_tag="VMZ53_27495"
     CDS             9882..10286
                     /locus_tag="VMZ53_27495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32288.1"
                     /translation="MRGLVLGVMLLGVGVGVAEAKPCPKGTSRIGKKCVIDRWPATPP
                     TTVKKLPPPTITLAVLHAQPASLDLATDKVVVESKPKPGAIYRAAQAYRAAGDRTKAL
                     ELYDQYLLVAPSGPAAPAVRAQIEKLRGDVPQ"
     gene            10315..11322
                     /locus_tag="VMZ53_27500"
     CDS             10315..11322
                     /locus_tag="VMZ53_27500"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HUS32289.1"
                     /translation="MDVIDAIGNTPLIELKKVAKGLPNRVFIKCEHLNPGGSVKDRTA
                     RALILDAEARGALVPDSDPKMTIIEGTAGNTGMGLALLALARGYRCICVIPEKVAADK
                     RVALRQLGAEVYVAKAAGIKSPDNFRNVAARLAQENGWFLANQFHNAANLNAHYGAPG
                     ISGTGPEIFQQMNGNIGAFVTAAGTGGTITGVGTFLKEKLPNVKIVMADPIGSSLGEW
                     VNTGELGPDGPFVIEGIGSASVPSNLHRAPLDYAEKIADAEALAMVERLVKEEGLTVG
                     GSTGVNVVAALRVAARTDLAGPVVTIAADLWDRYRSTQWMKDWEAREAQSLVFMEPYK
                     A"
     gene            11338..11880
                     /locus_tag="VMZ53_27505"
     CDS             11338..11880
                     /locus_tag="VMZ53_27505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32290.1"
                     /translation="MKAVLSYLLSLAGLGVSIWAVMGAALANLPYPKARLEIVNMLRT
                     SVNKAQFLCKTAKNTFYEPIGEAIKIGAMAQTTDPNIIGMATKPGYDSGVSSVTQHWN
                     ALFSRGKKGVMLVAGGVALAIVAKTNPALHIIVAVLTAGAAVWFMMVKLENERSLVRA
                     KAEILPEVDRGFAEGRYIAQ"
     gene            complement(11865..12434)
                     /locus_tag="VMZ53_27510"
     CDS             complement(11865..12434)
                     /locus_tag="VMZ53_27510"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS32291.1"
                     /translation="MPPDLVTPRLRLRQWRDADVEPFELMSNDREVMAHFPSLYSRDV
                     VETMVMRQRDFIAEKGYGFWAVDELATGRFIGFCGIKDVTFDVPWLPAIETGWRFARD
                     AWGKGYATEAGRAAQRFAFETLGLAELVAFLLPMNARSAAVCERLGMTRDPSRDFDHP
                     LIADGTISIAGFPQRRHVVYSLRRDHWAM"
     gene            complement(12436..13206)
                     /locus_tag="VMZ53_27515"
     CDS             complement(12436..13206)
                     /locus_tag="VMZ53_27515"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS32292.1"
                     /translation="MTKTDKHEAGSVCWVDLMTTDAEKARNFYKGLFDWTFFVGGPET
                     GHYSMGKLGDDNVAGLGQIQDGMKMPSAWTVYFASDDLDATIEKIKANGGSVMMGPMD
                     VMTEGRMGVFTDSTGVVFGVWQPMNHKGAGRVDEPGAMTWREVMTKDAAKAREFYGKV
                     FGLEAKKLEGGSMDYWTLHKGPNTVCGVMQMTDKHAHMPPHWNNYFAVANADAAAKKV
                     TELGGQVYAQPFDSPYGRIAIVADPTGAAFSIVQTPAT"
     gene            complement(13222..13620)
                     /locus_tag="VMZ53_27520"
     CDS             complement(13222..13620)
                     /locus_tag="VMZ53_27520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32293.1"
                     /translation="MRSWRSALFAIVGLVGACGTDVRPPDDEGGGDDSMIEPIAPNAC
                     DTTYLDYSNFGAPFVINWCRGCHSKSIPQGMRQKAPMTVNFDTYDDVQTWAERIEARA
                     ASMAPSMPPAGGPSEDERALLREWIECGTR"
     gene            complement(13604..14824)
                     /locus_tag="VMZ53_27525"
     CDS             complement(13604..14824)
                     /locus_tag="VMZ53_27525"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HUS32294.1"
                     /translation="MNRRDLFKLAGAAGALGMVGKLRPLSAATPATKKRLVMVLAQGG
                     WDVTYALDPKAPTKADVPVGARQLFGNLDVFTDASRPNVTSYFQKYAANTAIVRGISV
                     ASVAHQECLKRMSTGTRQETSADMAAIVAHDNGNDRPLPYLILGDTAYAGQYAVSAGR
                     VGARNQIVDLLDPSTTTAEDAILEKYATATANRIKATRGSLGYNKKRVDDFVEGIRRG
                     QKLQAVRDGFGTRGRTLALTSQIDLALDAFDRDISQSVMINTRLSWDTHDTNTDQSGF
                     HETTFAGLTRLLDGLCARPGSASGTTLLDETVVVCYSEFSRTPKLNAQMGKDHWPVTA
                     AMVIGAGVKGGRGYGASSDGIEAESVNFATGAKSASGMTLMSNHFVAGVLKLCGVTPT
                     DHLGATEVFDAFVA"
     gene            complement(14835..16535)
                     /locus_tag="VMZ53_27530"
     CDS             complement(14835..16535)
                     /locus_tag="VMZ53_27530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32295.1"
                     /translation="MRVLASLLLVAAACTDAANPSGDDDTTMPPDDVSPTAVYLTPAQ
                     HLTRASMVLRGIRPSLDELKQVAADPKALESIVDRYVDSPLFGQTIRELHNETLLMQI
                     EQPQFTFPPLGPIATATARQVNGRFDEPLRLIEDIVMSDQPYTKIVTADYTMADGTSA
                     AIWGLPHTGPAATWERVKFTDGRAPVGILSTNAIYDRWRSTGANYMRGRANVISRSLL
                     CHDFLASDIIIDTTVDLSDPAVVANAVVANSTCAGCHQTLDPLASHLFTFKRQIAPVS
                     VDSYPQTMFSPTTAQQWKTTNKRPPMFFGQTTGGTLSGLGIAIANDPRFAQCTARRFA
                     SYFTEVDRNDVSSAWIARLQKQFVDSDFDAKALAKAIVLSDEFRISHDTNAAEAETTV
                     GMLKVRPEQLQRMLRDITGYNWAVNSTTQLRGMPYGPTNLLESDYVGYRVLAGGIDSY
                     FVTEPVHTMNATSTLTAKLAASEAANFVVDHDAAAAAADRTLFTEAAVTATEPDRIRA
                     QLAHLHARIYGEVVAPDSPEVEDAYAMYDEAFQATNNRARAWKVVLIGMLSNVQSLFY
                     "
     gene            16671..17987
                     /locus_tag="VMZ53_27535"
     CDS             16671..17987
                     /locus_tag="VMZ53_27535"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS32296.1"
                     /translation="MSDIETQLQQRPGVQVVTQLRVEVVDGPDRGVNASGGDTLSVGT
                     ARDNALVISDFTVSRYHLEVSAKPGGILVTDLGSTNGTYVGAVRIERAFVPPGTNVTL
                     GGTTIRFDDAVKHTVPTSTDVRMYAGMIARSPGMLRLFSDIDRIATAPTSVLVVGESG
                     TGKERVAEALHTLSGRAAQPLVTIDCGALSSSLLASELFGHEKGAFTGADRVHVGAFE
                     RAGAGTVFLDEIGELPPSDQVSLLGVLERRRFRRVGGSQELELGARVIAATNRDLRAE
                     VNNNRFRHDLYHRLAVVVLRLPPLRDRREDIVPLVEHFARELGAAGPLEQVFGAETLQ
                     RWLKHPWPGNVRELRNAVEAALVVGPNAHFDHFDPQAPPASPDDPTQPLGPYNDARAV
                     VVRDFERNYLSRLLGEANGNVSQAARIAKMDRSHLIDLLQRHGLKG"
     gene            complement(17977..19494)
                     /locus_tag="VMZ53_27540"
     CDS             complement(17977..19494)
                     /locus_tag="VMZ53_27540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32297.1"
                     /translation="MVRALAVVAASALVGGCFDQTYTCNEDTQCDVGEAGRCEVTGFC
                     THYDSTCETGRRYSSHASALTDQCFDDRVEPINACAGGQGPAARTGCFADVCAQLPAC
                     CDVGWSDACAQLAQEACSTLRCDTRIAVNANRTSTMTNDRFEVLWDGAAWSIVPRAGY
                     GAPIAWIGPKPGDGAPRIAYTKPGALVVDSVDLPVDAAHSYNAITSVGFDRDRSDTIA
                     AAYSSPTGNGLEIWNVDTKTFRTVSTPSERLSWGDDNRDGFPDAITANAFSTYSFIDN
                     VDGDNHVRTLSSQANANTSGGGTPGAPAVSSIEWLDFDGDTNLDLVVFGSSVRLHTRA
                     EDLNDQAQFDLDCDPPSTAKTCSADPEPDLEQTAFFGTSFPTAAGRSLLASVFPDRKL
                     YRIQHQAGAAVATQLAFPNDTCTCVKTCSTTSCPGPNCTCTYDCNACASVLAIVARDL
                     DGDHALDIIAIDARLRIYTALAAGGYVFGAPTPMIAPLPALYTSVDVSVSGALIP"
     gene            complement(19488..>20413)
                     /locus_tag="VMZ53_27545"
     CDS             complement(19488..>20413)
                     /locus_tag="VMZ53_27545"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS32298.1"
                     /translation="TGDARGATTRGGAGGATSGGDAHRTSTRDARGATSGDARGATRG
                     GDARGAPSGGDARGAASSGGAGGATSGGDGRGLRVACMVAVIVAMSSGPRFAHADEPS
                     GPPTVLPGKLRDAYERGDYDEARRILLNMYALTPEPKLLFALGQVELNLEHYDAAIRY
                     YEKFLATDPPADQAALAQQAIGAARIKLAQPEKEAPPPPLPPEVKHYPPRRWYLEDTG
                     FVAFGGLAIGVGAGLVVYSHQLGTDHHGTLSQFDMRLDQARTMKWTGIGVASAGALVV
                     GVTVLRWRLRPDGGVMTAQVSPTGITVAGRW"
BASE COUNT         3501 a         6772 c         6691 g         3449 t
ORIGIN      
        1 agccgacctg gtcaccgccg tcgcatgact cgagcaactg gcagcccgcg caaccggcgc
       61 agccgacgtg gacgccaaca cgtcacgact cgagcaactg gcagcccgca caaccggcgc
      121 agccgacgtg gacgcccaca cgtcacgact cgagcaactg gcagcccgcg caacctgcgc
      181 agccgacgtg gacgccatcg cgacacgact cgagcaactg gcaacccgcg cagccgtcgc
      241 gtccgtcagc accgccgccg tctcggccgt cgtggtcgcc gccatcgcat tcgtcgagca
      301 gcagctcgtg gtcgccggcg tcgtcgagca gcagctcgtc gcggccatcg tcgtcatcgt
      361 cgtcatcgag ctcatcgtgg tcgcgcccgt cgtcatcatc gagcagcagc tcgtcgcggc
      421 cgtcatcgcc gtcgagcagc tcgcattcgt cgtcaagcgg cggctcgcac tcggcgcccc
      481 cgtcgagcgg cggcggcggg cactcgtctt ctcactccgg tggtggcggt ggacgccggc
      541 actaggcgcg agcgcgtctg gcgcgtggcg agagcgcgcg cgcgttcgct acttgcggaa
      601 cagccacttg tggcgaagca ggtacatcac gatcgcaacg ttgacgacga gcgcgatcac
      661 tttgccgacc gacgggtgct tgaacagctc gtagatctcc aacgggatca gtgacgacgt
      721 cacgatcacg gtgagccagt ccgcccacag atagcggcgg tgcaggcccc agccttcgag
      781 cgcgagcacg catgcataga tgatggcgcc gatgccgatc accgagatgc ggtggggcgt
      841 gaggccggcg agcttcgcga gcacgtgctg caccgtcggg cgacccgcat caatacccag
      901 ccacgcgatg agctccgtgc cgagcttggc gacgtcgctg tgaacgagcg cgaatgcggc
      961 gatgccgagt gcgaacagca gcacggcctt gatcgctttc cagacaataa tgacgcgaag
     1021 gccgaagtcg acgcgctcga cctcggtctt caccgtctcg gagatgtgcg ccgcgacttc
     1081 ttcgaccttg tgcgcgagcg gcgacgtcac tggatgcgat cgtagcgcga cgcgattaag
     1141 ctggctgcat gaagcgcgcg ctcgttgtgc tcggcctgtc gttcgtcggg tgcagcaatc
     1201 ccaacccgcc gcacaccgag cgtcccgtcg tgatgtcggg cggcgcgacg gttacgcgcg
     1261 ttgctcccga tacgctgccc gtgctcgatg catgggcgaa cgcgatgggc ggccgtgcag
     1321 cgatcagctc gatccgcgcg gtgcatgcga agggcagcta cgagcgcggc ggcatgaggg
     1381 gaacgatcga gctgtgggag acggcgagcg gccagcgccg cgaggagatc atccaggggc
     1441 cactgcgcga actgcgcgtg ttcgacggca cgcgtggctg gctcgtcgat cgcaatcgcg
     1501 aggtccgcga gctggccgcg ttcgagctcg acgatcagat gacgctcgca ttcatcggcg
     1561 cgcacgcagc gctgctcacc gatcgacgcg cgggcgacgt gaagctcgac ggcgacaagg
     1621 tcgtgctcgc gcccgatgga agtcgacggc ccgcgagcgt gtggctcgat cgcggcacgc
     1681 acctgcccga tcactacgag aagcgcgatg gcgagcgcac gcgcaagatc acgtggtcgg
     1741 actggaatcc cgtcggcaac acgggcgcga agctgccgtg gtcgcagcgc gaggagaacg
     1801 gcaatcccaa cgacgccgtc acgatccact ggacgaagct cgaagccgcg acaccacccg
     1861 acgctgcgtt cgcgcagcct gcggatcgcg atccggacat gtcgctcgcg agtgaaccgg
     1921 tgacggtgcc aatcgaggcg gtgtacggcg atccgagcgt gacgaaaggc ggcctgatct
     1981 tcgtcaaggt ctcgatcaac gatcagccga tgtcgttcat cctcgacacg ggcgccgagg
     2041 cgacggtcct caattcgtcg cgcgtcagca agctcggcct gcaaggcgtc ggcacgttcg
     2101 cgaccggcgc cggcggtggc gacgtggtgc tgtcgtacgt gccgggtgtc accacgaagg
     2161 tcggcggcgc gacggtgtcc aatcagatcg tcgcggcgat cctcctcgac gacatcgaga
     2221 agatgctggg ccggccgctc gacggaattc tcggctacga tttcttgtcg cggttcgtga
     2281 tcgagatcga ttacgcgaac cagcagatgc gcctgttcga tcgcacgaag taccaccaca
     2341 cgggatcggg caagccgatc gcgatcacgc tcgaagactc gacgccgttc ttcgacgcat
     2401 cggtcgagat tcccaagcgc ggcctgctcg ccgggcactt cgtgctcgac acgggctgct
     2461 tgtgcgacgt gcagctgttc acacccttcg tcgacgcgaa caacctgctc gccgcactgc
     2521 cggacgcaaa gcagagcgga atgtcggcag gcgccggcgg ccagacgaag gaagtatcgg
     2581 cgagcattcc gtcgattcac atcggcgacg tcgtcgtcaa agatgtgcga gcggatctcg
     2641 cgcgcgacac gttcggcgcg atggccgatc cagaatcgac gggcctcatc ggcgggctga
     2701 ccttcggacg cttcacgctc gtcctcgact acaagagcaa gcaggtcttc ctcgatccgg
     2761 ccaagtgact agcgccgctg cgtcagcgag taatagttgc ccgagtcgtc cttgaagaca
     2821 gcctcgagcc cgtacggcat ctccttcggc tcgcgcgtga actcgacgcc cttcgccttg
     2881 agctcgtcgt agtcgcgctt gcagtcgtcg gtcatgagca cgccaccacc gatcgcgccc
     2941 gcctcgacga gcgtgcgcaa cgagctcgca tgctcctcct tcatccacgg tcccggcttg
     3001 atcgcgtaca ggatcaggca gacgtcgggt tgggtcttcg gtccgacggt gagccagcgg
     3061 aagtccccca tgctcgcgtc gtcgcgaact tcgaagccga gcttctccgt gtagaacgct
     3121 ttagcgcggt cttgatcggt gacgtaaatg gtcgtgtgcg tgtgcttcgt gatcatgcgg
     3181 cactcgtacc gcgagtcgcc gcggccgact tcttcaaacg tgcgcgccgt acagaaagca
     3241 gctcgggata tagagaatcg gcacgccgag gctctgcacg aacgggcgcg cacgccgctg
     3301 atactcgcgc ggcgacagcc ccacgcgttt gcgaaacgtc gacgagaacg tgccgaggct
     3361 ctcgaagccg acttcgtggc agacctcggt gacactcgtc cccgcggcga gcgcgcggcg
     3421 tgcctgctcg aggcgaagct gcacgagata ttggtgcggc gagatgccaa acgtctgcgt
     3481 gaagttgcgg gcgagatgcg cgcgcgacac gccggacgcg gcagcgagct cgtcgagcgt
     3541 cgggttgcgt gcagcgtcgg catgcagcag atcgcgcgct cgcacgagac gtcgcagcag
     3601 cgctccgctt gccatccgcc agttctagcc ttcggagtgt gcgagcatca agacatggtt
     3661 cgcatcgcgc tgatcttgtt ggcgctcgcg gcgtgcaagc gcgagaaggc cggaccgggt
     3721 gccagcggcg ggcccggctc gggagttgtg acagccggct cggcttcgac aggctcagcg
     3781 gcgacaaccg cggcgtcagg ttctgcgggg tcgggctcgg cgaccgtggc aggacctatc
     3841 gatctcctgc atgcgatccc cgtgcgaatg gcggtgtcct cgctcgtcgc gaatccgaag
     3901 gtcagcgcgt acgacctcgt cgacggcgac ctcgcgacgg cgtggaactc gaagacgggc
     3961 cagctcaaag gtgcgtggat cgcgtttcgc gtaccaccga gcacgcacgt cgacgcgatc
     4021 gagctgactg ttggcttcgt gtctaagggc gcggagggcg actacttcac gctgaacccg
     4081 cgcatcaaga gcgtcgccgt gtggcacgac ggcattcggc tgcgcgaagt cgagctcgac
     4141 atcaacaagc gcgagctgca gaaagttccc gtcgatgcga acggcggcga ctacaagctc
     4201 gaggtcaccg cgttcgagcc cggcagcaag aagaactggc gcgagatctg tgtctccgag
     4261 ctgcgcgtga tcggcacgcc cgccccaggc acgaagacag gcgactactc gccgtcgatc
     4321 tcgatcgcat cgctcgatgc ggagccgctg ccggagccga acgtgacact cgaagtgctc
     4381 gacggcttcg cgtcggtcgc ggagttctgc gatgcgttca agaagcgcaa gccggcgccg
     4441 tgcgcgccat gggacgagaa gtgcgagaag acgcccgagc cgagcacgtg tggtgacgcc
     4501 gtgggcgagc ccctgacgct gccgcaactt ccgtcgggat ggaaagcgac atggtttgcg
     4561 tcgtatctcg gcaactcgaa ggtcgccgcg tgcaacctcg cgatcgaggc gaaaggccgc
     4621 ttctacgtgc tcgagcgcat cggcggcaat ggcgcgatag gtagcgcgtc gtgcggccaa
     4681 gcgatcgaca tgcagttgga tttggcgaag ccaacgcgat cgcgcagcga cattcgcgtc
     4741 gaggacgtca tggggacacc ggacaaggag ctcgtgctcc tcacgaccgt acccggcgat
     4801 atcgatccga tggacagcaa caagctgcca ttcacagaac agctgcacat ctgctcggcg
     4861 acgccttcgg gagagcccgc gtgcatgaaa gcaaagcttg gccagctcga cgtgaagaat
     4921 gcctccggcg atgacggcta cgcgcagtgg ctcgaagcgg cgtggctcga tgacttcgcg
     4981 atcgagcacg gccagctcgt gatcacgcgc aagagcggca aggcggagag ctacgagcaa
     5041 gacctcgcgc cgggccgcta tcgcttgaag ctgccggact agctgcggtg tgtaatcatc
     5101 cgtcggtggt gcgaacggcg gcgatgacga tgctcgcgct cgtcgcgtgc aagcgcgtcg
     5161 gcgatggtcc cgcggccaca ggctcgggtt cggcggcgcc gcccgtcatc tctgaagccg
     5221 gagtcgaggt cgcaaatgcc ggcagcgcag cgggtgttgc tcccagcggc tcggtcgact
     5281 tgcttcatgc agtgcccgcg cgtgttgcgg tgtcgtcgct cgtgaagaat cccgccatca
     5341 cgccatttca tctcgtcgac ggaagtctcg acaccgcctg gaactcgaag acgggcgagc
     5401 tcgtcggttc gtgggtcgcg tttcgcgtgc cggccgccgc gcacatcacc gagctccaga
     5461 tgacagcggg cttcgtatcg agcggggacg agggcgacta cttcacgctc aacccgcgca
     5521 tcaagcacgt cgcgatctgg cgcaacggcg cgaagctcgc cgaccgcgac ctcgacatca
     5581 ctgtacgcgc gctgcagaac atcccgatcg gcaacgaggg cggcgactac aagatcgaag
     5641 tcacggcgat cgagcccggt tccaagaaga actggcgcga gacctgtatc tcggagctgc
     5701 gagtgatggg aagcgcgacg ggcatgcgcc cgaaccacag cgttccggag atgtcgatcg
     5761 gttcgctcga cgccaagccc ctcgaagact ccgccttgta cttggtcccg ccgaaggact
     5821 tcggatcgat caaagacttc tgcgccactc tcgctgcgga cccgaagcgc acagccaaga
     5881 tcgtgtcgtg cggtgacgcc gcccaggccg ccctcgagct tccatcgccg ttgcccgctg
     5941 cgtggtcagc acactggttc tgcgcgaaga ccgagacgcc gagccccgcg aaggtgaacg
     6001 cagggaagat gatcaaacgt tcggaatgct tcctcgcggc cgaagccaac aatcggttct
     6061 tcatactgga ggttccaggc ctcttcgatc ctgtggtcac gatccgcgag agctacatga
     6121 atgtgggcga gaattcgcgc cgcagtgtcg agctcgtcga cgcagcggga agtcccgaca
     6181 aggatctgat cttcgtgacc acgtcgggcc cgacggagtg gctcaccatt tgtgctccga
     6241 cgccaacagg cgcaccggcg tgtggctcga agcagatcgc cgagatggag tttcacaaca
     6301 acaacagcga ttacggcttc gacgagtact acacgtacaa atggaggcgc gactaccagc
     6361 tcggtgacga gctcgatctc ggcagcgacg tgcctcccgt accgatccgc atcctcgaat
     6421 gaaggccctg ctgtacacca ccgccgtcgc cggcgtgctc gctgcgctct gcgtgctcgc
     6481 gatcgatcag tcgtttgcga agtggatctc gacgcgcgag acgtatcccg acgtctggaa
     6541 cacgatcatc ggttacctcg agtatccgct cggcgtcgag cccaacaagt ggatcggcgt
     6601 ctacgtgctc gtcgcgggtg cggtgctcac gctcgtcgtc acgccgctgc gcaagtacgc
     6661 gcatatctgg tcgctgctcg cgctctcgca cttactcggc cgcaacatga tcacgtggct
     6721 caagcctccg cttggtcgct ggcgtccgac cgagtggctc aagcaccacg cgtcgagcga
     6781 tacgttcttt cacaaggctg cctactcgtt tccatcgggc cacgtgatcc tgttcgcgag
     6841 catgatcttt ccgctcgcgc tcgcgtatcc gaaggcgagg ccgctgcttg gcgtcgtgtt
     6901 gttcgcgatg gtcgcgcgtg tcgcggtgaa cgcgcacttt ctcggcgacg tcttcggcgg
     6961 tctcgcgctc agcgcgatcg ggacgtgggc gtgtgcggtg gcggtgcgtc gcgctttgcc
     7021 gtcgcagata cgaccaccgt acctccaatg atcagcgccg cgccggcgag cgtaccgagc
     7081 gcgggtgttt gctcgaacaa cagcagctgc cagaggagtg cgaagcagat ctggatgtag
     7141 ccgacggacg tcgcgcgccc tgcccgctcg atcgtgagcc ccatcgtcaa gaacacctgg
     7201 ccgatctgcg tcgcgatgcc gatgccgatc agcaacagcc aggcgcgagc gtcgggcatc
     7261 acgaagttgg ccgcggccca cggaatcgcg agcggtgtgg cgacgagcgg gaagtagaag
     7321 acgatgacga gcggatgctc ggtgcgcgac agctggcgca cggtgacgta cgcgatcgac
     7381 gacagcgtcg cggaggcgac agcgacgagc gagccgaccg gatcgtcgac ggtgccaatg
     7441 cccggatgcg cgaccgcgac gacaccagca acaccgcacg cgatcgcgaa cgcagcggcc
     7501 cagccgattt tctcgtcgag aatccaccac gcgagcacgc ccgtaatcag cggctggatg
     7561 taatgcagcg tcgtcgcgtc cgcgagcggc aaccgcgcga gcgcgatgta gtaacagccg
     7621 agcgccgaga atccgagaag accgcggaac acgagcttgc cgcgctgcac gccccacggc
     7681 gagagcggct tgccatcgct gccgaccgcg cgcttcacca tcacgtacga cagcacgagc
     7741 gtcatgagcg cgcgcgcgag cacgatctcg cccgtcggca gccgcggcgc ggcgagcttc
     7801 acgagcacgc tcatcaccga gaagccgagc gcggacagcg tcatgtagag gacgccgcgc
     7861 gacttcatgc agagctactg tgcgatagca gctgcgagat ggcgagcaat gagcgggtga
     7921 atctcgacct tcggcttgta cgtcgccatg aggccagcca ctgacgcgag tacgcgaccg
     7981 agcagcacga acgagcgcgg gataacgagc ccgccgagtt gcttggcctg cgcgatctga
     8041 tcggcaaatg ccgcctgcca atcgagctcg ctcgcgttca tgcccggctt catcgcgccg
     8101 atgagcgccg aggtcagatc gacgagctgc tcggggtcgt cggccgagaa cccgagcgtc
     8161 gcgagctcct tgccggcagc cgcgtgattg ccgcccgcga tcgcgagcac gaggcgcgca
     8221 tacgccgccc tctccggctt gctcagctcg agcatgcaac cgaagtcgag gagcgcgagc
     8281 tttccgtcgg ccgtgacgag gaagttgccg ggatgcgggt cggcgtggac ctgaccgcgc
     8341 acgaggattt gcgcggcgac ctcgccgacg agctcgccga gcagccggtc tcgcgcctcg
     8401 ggcgtcgcct tctcgatcca actcgtcagc ttttcgcctt cgatgcgcga catcgtcagc
     8461 acgcgcttgc tcgatgcgtc gggatgcggc cgcggaacga cggcgctgcc cgtgtagccc
     8521 cacagcgagt cagcctccgc ctcgtagtcg agctcgaccg cgagcgcacg cgcgagctcc
     8581 gtcgcgagag ttggtagatc aacgcccggc acctcgccga ccgtcgatgc gatcgtcttc
     8641 agcgccgcga tatccgccgc gatcacgtcc tcgatgccgg gcacctggac cttcacgacg
     8701 acgcgctcgc cgcgcccgcc ctccgccgcc ttgtcgcgca gcacggccgc atgcacctgc
     8761 gccaagctcg ccgccgcgat cggtacatca tcgaattcga cgaacagctc gtcgatggag
     8821 cgaccgagct cgctctcgat gcgcgcgcga atcgctgcag cgtcgaccgg tggcacttcg
     8881 tcttgcagct tcgcgagctc ggccgcccat acgggtccga tcatgtcggg cctacacgat
     8941 gcgagctggc cgatcttcgc gatgccgccg cgcagctccg ccgcatacgc ggccgtgcgt
     9001 accgcgagat cgcgatgatc ttcgggccgc agctccggcg agccggtgcg cgccgcctgc
     9061 gccaatcgaa gccagcgctg ccgcgcaaag agcgcagccg cgacggtcgc aatcttcgtc
     9121 atgcgtggcg tcgctttcgt gaagctgctc gcttgatcac gcgcgtgcac ggcttcatcg
     9181 cgcgctgcac cggcaagtgc ttgcgcgtga cgtacgacgg caccgccatc acgtgcgaga
     9241 tcgagtgcca tcgcatcgag ccgcgtgccg agatgctgga ccaactttgc cgcgttcgtc
     9301 gcggtctcgc gcaatggctg cagtagctct tcgagcgtcg gattcatagg tctccttatt
     9361 tgcggagctc ggccaaacac ttcgcgcaca cgatcgcgcg gtcgccgccg atcgagatcg
     9421 atgcgtcact gcccttcggg aggatgtcgt tgcatcgcga gcagatcgcg ttcttcgcga
     9481 gcacgatcgg ctgccacgcg atcacctcat cgggctccgt cggatgcgcc tttgcgcgcg
     9541 catccccgcg agcggcacgc gcgatcgcat ggctgtccgc caccacatcg ccgaccaggc
     9601 cgaacgcgtg gccgagcacg ttgcgcacga ggttcgacac ggacatcccg agctcttgcg
     9661 cgcgttgccg caattcggcc tcgaggctct ccgggacgcg cgtatgtatc acgcgctctt
     9721 tcttctccgg ctcgtccgtg tcgtcgggcg ccggcgtggg tggacgcttg cgtgtcacga
     9781 tgtgacacaa tatgtatcac gatgtgacac gttgtcaaca ccggggtgcg cgcctgcatt
     9841 tcgtttgcct tggcgaaggc gacaggcgta gggtccggcc catgcgtggg ctggtgcttg
     9901 gcgtgatgct ccttggagtc ggggtaggcg tggccgaagc gaagccgtgt ccgaagggga
     9961 cgagccgcat cggcaagaag tgcgtcatcg atcgatggcc tgcgacgccg cccacgacgg
    10021 tgaagaagct gccgccgccg acgattacgc tcgctgtgct gcacgcgcag ccggcgtcgc
    10081 tcgatctcgc gaccgacaag gtcgtcgtcg agagcaagcc aaaacccggc gcgatctatc
    10141 gcgcggcgca ggcgtatcgc gcggccggtg atcgtacgaa ggcgctcgag ctctacgatc
    10201 agtatctact tgtcgcgccg tccggccccg cagctcccgc ggttcgcgcc cagatcgaaa
    10261 agctccgcgg cgacgttcct cagtagtccg tcgcgcggct atggtcggcg ggccatggat
    10321 gtcatcgacg cgatcggcaa caccccgctc atcgagctga agaaggtcgc caagggcctg
    10381 ccgaaccgcg tgttcatcaa gtgcgagcac ttgaaccccg gcggcagcgt gaaggaccgg
    10441 accgcgcgcg cgctcatcct cgatgcggaa gcccgcggcg cactggtgcc ggactccgac
    10501 ccgaagatga cgatcatcga ggggactgcg ggcaacaccg ggatgggctt ggcactgctc
    10561 gcgctcgcgc gcgggtatcg ctgcatctgc gtgattcccg agaaggtcgc cgcggacaag
    10621 cgcgtcgcgc tgcgccagct cggcgccgag gtctacgtag cgaaggccgc cggcatcaag
    10681 agcccggata acttccgcaa cgtcgcggcg cgtctcgctc aagagaacgg ctggttcctc
    10741 gcaaaccagt tccacaatgc agcgaacttg aacgcgcact acggtgcgcc cggcatctcg
    10801 gggaccggcc ctgaaatctt tcagcagatg aacggcaaca tcggcgcgtt cgtgacggcg
    10861 gcgggaacgg gcgggacgat caccggcgtc ggcacgttct tgaaagagaa gctgccgaac
    10921 gtaaagatcg tgatggccga tccgatcggg tcgagcctcg gcgagtgggt gaacaccggc
    10981 gagctcgggc ccgacggacc gttcgtcatc gaagggattg gctccgccag cgtgccgagc
    11041 aacctgcatc gcgccccgct cgattacgcc gagaaaatcg cagatgccga ggccctcgcg
    11101 atggtcgaga ggctggtaaa ggaagagggc ctcaccgtag gtggttcgac gggcgtcaat
    11161 gtcgtcgctg cgctccgggt cgccgcgcgc acggatctcg ccgggccggt cgtgacgatc
    11221 gccgcagacc tctgggatcg ctaccgctcg acgcagtgga tgaaggattg ggaagcgcgc
    11281 gaggcgcaga gcctcgtgtt catggagccg tacaaggcgt agtggtacgc tgaggacgtg
    11341 aaggcagtcc tgagctacct gttgtcgctc gctggcctcg gcgtttcgat ctgggcggtc
    11401 atgggcgcgg cgctcgcgaa tctgccgtat cccaaagcgc ggctcgagat cgtgaacatg
    11461 ctgcgcacga gcgtgaacaa ggcgcagttc ctgtgcaaga ccgcgaagaa cacgttctac
    11521 gagccgatcg gtgaagcgat caagatcggc gcgatggcgc agacaaccga tccaaacatc
    11581 atcggcatgg cgacgaagcc tggctacgac tcgggcgtga gctcggtgac gcagcattgg
    11641 aacgcgctgt tcagccgcgg caagaagggc gtgatgctcg tcgccggtgg cgtcgcgctc
    11701 gcgatcgtcg cgaagacgaa tcctgcgctc cacatcatcg ttgccgtgct caccgcgggc
    11761 gcagccgtgt ggttcatgat ggtgaagctc gagaacgagc gctcgctggt ccgcgcgaaa
    11821 gcggagatcc ttcccgaggt cgaccgcggc ttcgccgaag gccgctacat cgcccagtga
    11881 tcgcgacgta gcgagtacac gacgtggcgg cgctggggaa agccggcgat ggagatggtg
    11941 ccgtcggcga tcagcggatg gtcgaagtct cggctcggat cgcgcgtcat gccgaggcgc
    12001 tcgcagaccg ctgccgaccg cgcgttcatc ggcagcaaga acgcgacgag ctctgcgagg
    12061 cctaatgtct cgaacgcgaa gcgctgcgcg gcacggcccg cttccgtcgc atagcctttg
    12121 ccccacgcat cgcgcgcaaa ccgccagccg gtttcgatcg ctgggagcca cggcacgtcg
    12181 aacgtcacgt ccttgatgcc gcagaacccg atgaagcgcc cggttgcgag ctcgtcgacg
    12241 gcccagaagc cgtagccctt ctcggcgatg aagtcgcgct gccgcatcac catcgtctct
    12301 acgacgtcgc gcgagtacag cgacgggaag tgcgccatga cctcgcggtc gttgctcatc
    12361 agctcgaacg gctcgacgtc ggcgtcgcgc cactggcgaa gccgcaatct tggcgtgacg
    12421 agatcgggcg gcacgctacg tcgcaggcgt ctgcacgatc gagaacgcgg cgcccgtcgg
    12481 gtctgccacg atcgcgatcc gaccgtacgg cgaatcgaac ggctgcgcgt acacctggcc
    12541 gccgagctcg gtcaccttct tcgcggcagc atcggcattc gcgacggcga agtagttgtt
    12601 ccagtgcggc ggcatgtgcg cgtgcttgtc ggtcatctgc atgacgccgc agacggtgtt
    12661 cgggcctttg tggagcgtcc agtagtccat cgatccgccc tcgagcttct tcgcttcgag
    12721 gccgaagacc ttgccgtaga actcgcgcgc cttcgcagcg tccttcgtca tgacttcgcg
    12781 ccacgtcatc gcgcccggct catcgacgcg acccgcgccc ttgtgattca tcggttgcca
    12841 gacgccgaac acgacgccgg tcgaatccgt gaagacgccc atgcgcccct cggtcatgac
    12901 gtccatcggg cccatcatca cgctgccccc attcgctttg atcttctcga tcgtcgcgtc
    12961 gaggtcgtcg ctcgcgaagt acaccgtcca cgcggacggc attttcatcc catcttggat
    13021 ctggccgagg ccggcgacgt tgtcatcgcc gagcttgccc atcgagtagt gtccggtttc
    13081 cggaccaccg acaaagaacg tccagtcgaa caaccccttg tagaagttgc gcgccttctc
    13141 tgcgtccgtg gtcatgagat cgacccagca gacgctgccc gcttcgtgct tgtccgtctt
    13201 cgtcatgcgg cgaggctagc ctcaccgcgt gccgcactcg atccactccc gtaacaaagc
    13261 gcgctcgtct tcgctcggtc caccggcagg cggcatcgac ggcgccatcg acgcagcgcg
    13321 cgcttcgatg cgctccgccc acgtctggac gtcgtcgtac gtatcgaagt tcaccgtcat
    13381 cggcgccttc tgccgcatgc cctgcgggat cgatttcgag tgacaaccac ggcaccagtt
    13441 gatgacgaac ggtgcgccga agttgctgta gtcgaggtac gtcgtgtcgc acgcattcgg
    13501 cgcgatcggc tcgatcatgc tgtcatcgcc gccgccttcg tcgtcgggag gacgcacgtc
    13561 ggtgccgcac gcgccgacga gcccgacgat cgcgaacaat gcgctacgcc acgaacgcat
    13621 cgaagacctc cgttgcgccg aggtgatcgg taggcgtcac gccgcacagc ttcagcacgc
    13681 cggcgacgaa gtggttcgac atcagcgtca tgccgctcgc gctcttcgca ccggttgcga
    13741 agttgacgct ctcggcttcg atgccatcgc tgctcgcgcc gtagccgcgc ccgcccttga
    13801 cgcctgcgcc gatcaccatc gccgcggtca cgggccagtg atccttgccc atctgcgcgt
    13861 tgagcttcgg cgtgcgcgag aactccgagt agcaaacgac gactgtctcg tcgagcaacg
    13921 tcgttccgct cgcgctccct ggacgggcgc agaggccatc gaggagccgc gtgagaccgg
    13981 cgaacgtggt ctcgtggaag cccgactggt cggtgttcgt gtcgtgcgtg tcccacgaca
    14041 ggcgcgtgtt gatcatcacc gactgcgaga tgtcgcgatc gaacgcatcg agcgcgaggt
    14101 cgatttggct cgtcagcgcg agcgtgcgac cacgcgtgcc gaagccgtcg cgcaccgcct
    14161 gcagcttctg gccgcggcga atgccctcga cgaaatcgtc gacgcgcttc ttgttgtagc
    14221 cgagcgatcc gcgcgtcgcc ttgatgcgat tggcggtggc cgtcgcgtat ttctcgagga
    14281 tcgcgtcttc tgccgtcgtc gtgctcggat cgagcaggtc gacgatctgg ttgcgggcac
    14341 cgacgcggcc cgcgctcacc gcgtactgac ccgcatacgc cgtgtcaccg agaatgagat
    14401 acggcagcgg acgatcgttg ccgttatcgt gcgcgacgat ggcggccatg tcggcgctcg
    14461 tctcttggcg cgtgcccgtc gacatgcgct tgagacactc ctgatgcgcg acgctcgcga
    14521 ccgagatgcc gcgcacgatt gcggtgttcg cggcgtactt ctggaagtac gaggtcacgt
    14581 tcgggcggct cgcgtccgtg aagacatcga ggttgccgaa caactggcgc gcaccgacgg
    14641 gaacgtccgc cttcgtcggc gcctttggat cgagcgcgta cgtgacgtcc cagccgccct
    14701 gcgcgagcac catgacgagg cgcttcttcg tagcgggcgt tgcagcagac agcggacgca
    14761 gcttgccgac catgccaaga gcaccagcgg cgcctgcgag cttgaaaaga tctcgacggt
    14821 tcatcgtgat gctcctagta gaaaaggctt tggacgttgg acagcatgcc gatgaggacg
    14881 accttccacg cacgagcgcg gttgttcgtc gcctggaacg cctcgtcgta catggcgtag
    14941 gcgtcctcga cctccggcga atcgggcgcg accacctcgc cgtagatgcg cgcatgcaag
    15001 tgcgcgagct gggcgcggat gcgatccggc tccgtcgcag tgacggcggc ttcggtgaac
    15061 agcgtgcgat ccgcggcggc ggcggcggca tcgtgatcga cgacgaagtt tgcagcttcg
    15121 gatgcggcga gcttcgcggt gagcgtcgac gtcgcgttca tcgtgtggac gggctcggtg
    15181 acgaagtagc tgtcgatgcc gcccgcgagg acgcgatacc cgacgtagtc gctctcgagc
    15241 agattcgtcg ggccgtacgg catgccgcgc agctgcgtgg tgctgttgac cgcccagttg
    15301 tagccggtga tgtcgcgcaa catgcgctgc agctgctcgg gccgaacctt gagcatcccg
    15361 acggttgtct ccgcctccgc cgcattcgtg tcgtgcgaga tgcggaattc gtcggagagc
    15421 acgatggcct tcgcgagcgc cttcgcatcg aagtccgaat cgacgaactg cttctgcagg
    15481 cgagcgatcc acgccgacga cacgtcgttg cgatcgacct cggtgaagta cgacgcgaac
    15541 cggcgcgcgg tgcactgcgc gaaccgcgga tcgtttgcga tcgcgatgcc gaggcccgac
    15601 aaggtgccgc cggtagtctg gccgaagaac atcggcgggc gcttgttcgt cgtcttccac
    15661 tgctgcgcgg tcgtgggcga gaacatcgtc tgcggataac tgtcgacgga gaccggcgcg
    15721 atctggcgct tgaatgtgaa cagatggctc gcaagtggat cgagtgtctg gtgacaacca
    15781 gcgcacgtcg agttcgcgac cacggcgttg gcgacgaccg ccggatccga taggtcgacg
    15841 gtcgtatcga tgatgatgtc gctcgcgagg aagtcgtggc acagcagcga gcggctgatc
    15901 acgttcgcgc ggccgcgcat gtagttcgcg ccagtcgagc gccagcgatc gtagatcgcg
    15961 ttggtcgaga gaatgccaac gggcgcgcga ccatccgtga acttcacgcg ttcccacgtc
    16021 gcggcaggcc cggtgtgcgg caagccccag attgcggccg aggttccgtc ggccatcgtg
    16081 taatcggccg tgacgatctt cgtgtacggc tgatcggaca tcacgatgtc ttcgatcaag
    16141 cgcagcggct cgtcgaagcg gccgttcacc tggcgcgcgg tcgcagtcgc gatcggcccc
    16201 agcggcggaa acgtgaactg cggctgctcg atctgcatca gcagcgtctc gttgtgcagc
    16261 tcgcgaatcg tctggccgaa cagcggggag tcgacatagc gatcgacgat cgactcgagc
    16321 gccttcggat ccgcggcgac ttgcttcagc tcgtcgagcg acggccggat gccgcgaagc
    16381 accatcgatg cgcgcgtcag atgctgcgcg ggcgtcaggt acaccgcggt gggactgaca
    16441 tcgtccggcg gcatggtcgt gtcgtcgtcg ccgctcggat tggccgcgtc cgtgcacgct
    16501 gcggccacga gaaggagtga agccaggact cgcatgatgg gggcatctct cagcaagggt
    16561 gggaccagtc gtcatgcaga cctctcgcgc gtttgtgtgg ttaactggga tcggtgttgt
    16621 cgggagtccc cagcacgttg tagggacaac ccgacagatc attcacgacg atgagcgata
    16681 tcgaaacgca gcttcagcaa cgccccgggg tacaggtcgt cacgcagctg cgcgtcgagg
    16741 tcgtcgacgg acctgatcga ggcgtcaacg ccagcggcgg cgacacgctg tccgtcggca
    16801 ctgcacgcga caacgcgctc gtgatcagcg acttcacggt ctctcgctat cacctcgagg
    16861 tttcggcgaa gcccggcggc attctcgtta ccgatctcgg ctcgacgaac ggcacgtacg
    16921 tcggcgcagt tcgcatagag cgcgcattcg ttccaccggg cacgaacgtc acgctcggcg
    16981 gcaccacgat ccgcttcgac gatgcggtga agcacacggt gccgacgtcg accgacgtcc
    17041 gcatgtacgc gggcatgatc gcgcgctcgc cgggcatgct gcgcttgttc tccgatatcg
    17101 atcgcatcgc aacggcgccg acgtcggtgc tcgtagtcgg cgagtcgggc acgggcaaag
    17161 agcgcgttgc cgaagcgctg cacaccctat cgggccgcgc cgcgcagccg ctcgtcacga
    17221 tcgattgcgg cgcgctgtca tcgagtctgc tcgcgagcga gctgttcggt cacgagaagg
    17281 gcgccttcac tggtgcggac cgcgtgcacg tcggtgcgtt cgagcgcgcg ggcgccggta
    17341 ccgtgttcct cgacgagatc ggcgagttgc cgccatcgga tcaagtctcg ctgctcggcg
    17401 tgctcgagcg ccgccggttt cgccgcgtcg gtggctcgca agagctcgag ctaggcgctc
    17461 gcgtgatcgc ggcgaccaat cgcgacctgc gcgccgaggt caacaacaac cgcttccgtc
    17521 acgacctcta ccaccgcctc gcggtcgtcg tgcttcgcct gccgccgctg cgcgatcgcc
    17581 gcgaggatat cgttccgctc gtcgagcact tcgcgcgcga gctcggtgcg gcaggtcctc
    17641 tcgagcaggt gttcggcgcc gagacgctgc agcgttggct caagcacccg tggccgggca
    17701 acgtgcgcga gctgcgcaac gcggtcgaag ctgcgctcgt cgtcggccca aacgctcact
    17761 tcgatcactt cgatccgcag gccccacccg cttcgccgga cgatccgacg cagccgctcg
    17821 ggccatacaa cgatgcacgt gccgtcgtcg tgcgcgactt cgagcgcaac tacctctcgc
    17881 gactactcgg cgaagcaaac ggcaacgtct cgcaggccgc tcgcatcgcg aagatggatc
    17941 gctcgcacct gatcgatcta ctccagcgcc acggcctcaa gggatgagcg cgccgctgac
    18001 cgatacgtcg acgctcgtat agagcgccgg cagtggggcg atcatcggcg tcggtgcgcc
    18061 gaacacgtag ccgcccgcag cgagcgcggt gtagatgcgc agcctcgcgt cgatcgcgat
    18121 gatatcgagc gcgtgatcgc cgtcgagatc gcgcgcgacg atcgcgagca ccgacgcaca
    18181 cgcattgcag tcgtaggtgc acgtgcagtt ggggcccgga caactcgtcg tgctgcaggt
    18241 cttcacgcac gtgcaggtgt cgttgggaaa cgcgagctgc gtcgcgacgg cggcgcctgc
    18301 ctggtgctgg atccgataga gcttgcggtc ggggaagacg cttgccagaa gagagcgccc
    18361 cgctgccgtc ggaaaggacg tcccgaagaa cgcggtctgc tcgagatccg gctcgggatc
    18421 cgcggaacat gtcttcgcgg tgctcggggg atcgcagtcc agatcgaact gggcttggtc
    18481 gttcagatcc tccgcacggg tatgaaggcg caccgacgat ccgaacacga cgagatcgag
    18541 gttcgtgtcg ccatcgaaat cgagccactc gatcgagctg actgccggcg ctccgggcgt
    18601 gccgccgccg ctcgtgttcg cgttggcctg gctcgagagc gttcgcacgt gattgtcgcc
    18661 atcgacgtta tcgatgaacg aatacgtcga gaacgcgttc gcggtgatcg catcgggaaa
    18721 cccatcgcgg ttgtcgtcgc cccacgacag ccgttccgac ggtgtgctca cggtgcgaaa
    18781 cgtcttggta tcgacattcc agatctcgag cccgttgccg gtcggcgacg aatacgcggc
    18841 cgcgatcgta tcgctgcgat cacgatcgaa tccgacggac gtgatcgcgt tgtacgagtg
    18901 cgcggcgtct acgggtaagt cgacgctatc gacgacgagc gcaccgggct tcgtgtacgc
    18961 aatgcgcggc gcaccatcgc caggcttcgg tccgatccac gcgatcggcg cgccataccc
    19021 tgcgcgcggc acaatgctcc acgcggcgcc atcccacagc acctcgaagc gatcgttcgt
    19081 catggtgctc gtgcggttcg cattgacggc aatgcgcgta tcgcagcgca gcgtcgagca
    19141 cgcctcctgc gcgagctgcg cgcacgcatc gctccagcca acatcgcagc acgcgggcag
    19201 ctgcgcgcag acgtcggcga aacacccggt ccgtgccgcg ggtccctgcc cgcccgcgca
    19261 cgcattgatc ggctcgacgc gatcgtcgaa gcactgatca gtcaacgccg aggcgtgcga
    19321 cgaatatcgg cgtcccgtct cgcacgtcga atcgtagtgc gtgcagaagc ccgtcacctc
    19381 gcagcgtccc gcctcgccga catcgcactg cgtgtcctcg ttgcacgtgt atgtctggtc
    19441 gaagcagccg ccgactagcg cgctggccgc gacaaccgcg agcgctctca ccatcggccc
    19501 gccacggtga tgccggtcgg cgagacctgc gctgtcatga cgccgccatc cggccgcagt
    19561 cgccaacgca gcaccgtcac accgacgacg agcgcccccg ccgacgccac accaatcccg
    19621 gtccacttca tcgtgcgcgc ctgatcgagc cgcatgtcga actgcgacag cgtgccgtga
    19681 tgatcggtgc cgagctggtg gctgtagacg acgaggcccg cgccgacgcc aatcgccagg
    19741 ccgccgaacg cgacgaagcc cgtatcttcg aggtaccagc gccgcggcgg gtagtgcttc
    19801 acctcgggcg gcagtggcgg aggcggcgcc tccttctcgg gctgtgcgag cttgatgcgc
    19861 gccgcgccga tcgcttgctg cgcgagagcc gcctgatccg ccggcggatc cgtcgcgaga
    19921 aacttctcgt agtagcgaat ggccgcgtcg tagtgctcga ggttgagctc tacctgccct
    19981 agcgcaaaca agagcttcgg ctccggcgtc agcgcgtaca tgttcaacag aatgcggcgc
    20041 gcttcgtcgt agtcgccgcg ttcgtacgcg tcgcgcagct tccccggcaa caccgtcgga
    20101 ggcccagaag gctcgtccgc gtgcgcaaac cgcgggcccg agctcatcgc gacgatcacc
    20161 gcaaccatgc acgcaacccg caaaccacgc ccatcgccgc cgctcgtcgc accgcccgca
    20221 ccgccgctgc tcgctgcacc acgcgcatcg ccgccgctcg gcgcaccacg cgcgtcgccg
    20281 cccctcgtcg caccacgcgc atcgccgctc gtcgcaccac gcgcatcgcg tgtgctcgtg
    20341 cgatgggcat cgccgccgct cgtcgcaccg ccggcaccgc cgcgggtcgt cgcaccacgc
    20401 gcatcgcctg tgc
//
LOCUS       DATLBZ010000548         3471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_720790, whole genome shotgun sequence.
ACCESSION   DATLBZ010000548 DATLBZ010000000
VERSION     DATLBZ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720790"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VMZ53_27550"
     CDS             complement(<1..292)
                     /locus_tag="VMZ53_27550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32299.1"
                     /translation="MKDGLVEKVHHWPGPKIVQAFLADKTLKARRPRFLFRDDGPMPE
                     EVELALTIPKELGNHDDIVERVRAGIAAVEQECSQERAKNNRCVFGRRRILRQ"
     gene            623..2155
                     /gene="istA"
                     /locus_tag="VMZ53_27555"
     CDS             623..2155
                     /gene="istA"
                     /locus_tag="VMZ53_27555"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HUS32300.1"
                     /translation="MRKTKEILRLKWQHERSHREIARALAVGVGTPSDVAKRAIAAGL
                     TTWEAVAALSDEDLDRKLYAEQPRSPAPARPKPDPATLHIELRRPGVTLRLLHEEYAA
                     AHPDGYGYTQFVAHYNAWADKQKVVMRQVHKAGEKCFVDYAGKKPWIVDPNTGERVEL
                     ELFVAVMGASNYTYAEVTATQQSPDWIASHSRLVEYLDGAPGAFVPDQLKTGVTIASR
                     YEPAIQRTYEEWSQHYSTTILPARPASPRDKAKVEGGVLIAERWILGRLRNLTCFSID
                     EMNEQIAALLVELNSRVMKRYGKSRRQLFDELDRPVLAPLPPSRFTYGDWRFGTVGLD
                     YHIAVDHHDYSVPYTLAEEYVESRLTATTVEIFFKGDRRASHIRSFEYGGTTTLVEHM
                     PASHRKYREESVVELAEWAARVGPCTHALVRAILDEKRHPEHGFRSCRGLKSLTKRYT
                     PERIEAACERALVVGARSYLNVESILRRGLDKQPLTELTPSADEPPALEHENIRGPDY
                     YH"
     gene            2163..2909
                     /gene="istB"
                     /locus_tag="VMZ53_27560"
     CDS             2163..2909
                     /gene="istB"
                     /locus_tag="VMZ53_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HUS32301.1"
                     /translation="MLKEPTIEKLKAMRLDAMASAWSEQHGKPDVNRLSFDERFGMLV
                     DAEWLHRENRRLTKALRDAKLKLASACLEDIEYSSKRELDRAQMRQLASCRWVVEHQN
                     VLITGMTGTGKTYLACALAQQACRKGHRAIYRRASRLFSELALARADGTYARLLTRFA
                     RADVLVLDDWAMAPVTDSERRDLLEVLEDRYGTRSTIVTSQVPPEKWHKHIADPTHAD
                     AICDRLIHNAHKLVLKGPSKRKGEEAETEK"
BASE COUNT          675 a         1145 c         1100 g          551 t
ORIGIN      
        1 cctgacgcag gatgcggcgg cgaccgaaca cgcaccggtt gttcttggcg cgctcctgtg
       61 agcactcctg ctcgaccgca gcgatcccag cgcgaacgcg ctcgacgatg tcgtcgtgat
      121 tgccgagctc ctttgggatc gtgagggcga gctcgacctc ttcaggcatc ggcccgtcgt
      181 cacgaaacaa gaatcgcgga cgccgtgcct tgagcgtctt gtccgcgaga aacgcttgca
      241 cgatcttggg ccccggccag tggtgaacct tctcgaccaa tccatccttc acagggttgg
      301 tggccgtgta gatgatcttc tcgactacga tgtcgatatc aaccagctcg acgaggcaag
      361 gctcgttgcg taattcagtc gatcgcgatc accgaaaacg gtccatcgcg atcaccgaaa
      421 acggtccatc gcgatcgccg aaaacggtcc atcgcgatca ccgaaaacgg ttcatcgcga
      481 tcggtggtcc ggccaatcgg ctggacctga actgatcaga gggtgggatc aggctgggcg
      541 ctgctgagcg tctggtcgac gtcgcgggga cggtgcttcg gggcgctcca agaggagcgc
      601 gatgccgacg gagcgactgt ccatgcggaa gacgaaagag atcctgcgac tgaagtggca
      661 gcacgaacgc agccatcgag agatcgcgcg tgcgctcgcc gtcggtgtcg ggacgccgag
      721 cgatgtcgcg aagcgcgcga tcgcggcggg cctcacgact tgggaagccg tcgcggcgct
      781 cagcgacgag gatctcgatc gcaagctcta tgcggagcag ccgcgctcgc cggcgccggc
      841 acggcccaag cctgatccgg cgacgctgca catcgagctg cgccgaccag gcgtgacgct
      901 gcgacttctc cacgaggagt acgcggctgc gcatccggac ggctacgggt acacgcagtt
      961 tgtcgcgcat tacaacgcgt gggccgacaa gcagaaggtc gtcatgcgcc aggtccacaa
     1021 ggctggcgag aaatgcttcg tcgactacgc cggcaagaag ccgtggatcg tcgacccgaa
     1081 cacgggcgag cgcgtcgagc tcgagctctt cgtcgcggtg atgggcgcgt cgaactacac
     1141 ctacgccgag gtaacggcaa cgcagcagtc acccgactgg atcgcgagcc acagccggct
     1201 cgtcgagtac ctcgatggcg cgccgggtgc tttcgtcccc gatcagctca agaccggcgt
     1261 caccatcgcg agccgatacg agccggcgat ccagcgcacg tacgaagagt ggtcgcagca
     1321 ctactcgacg acgatcctgc ccgcacgacc cgcgagtcct cgcgacaagg ccaaggtcga
     1381 aggcggcgtg ctgatcgccg agcgctggat cctgggtcgc cttcgcaatc tgacgtgctt
     1441 ctcaatcgac gagatgaacg aacagatcgc agcgctgctc gtcgagctga acagccgcgt
     1501 gatgaagcgc tacggcaaga gcaggcgtca gctcttcgac gagctcgatc gccccgtgct
     1561 cgcgccgctg ccgccgtcgc gcttcaccta cggcgattgg cgcttcggca ccgtcgggct
     1621 cgactaccac atcgctgtcg accaccacga ttactcggtg ccgtacacgc tcgccgagga
     1681 gtacgtcgaa agccgactga ccgcgacgac cgtggagatc ttcttcaagg gcgaccgtcg
     1741 tgcctcgcac atccgcagct tcgaatacgg cggcacaacg acgctcgtcg agcacatgcc
     1801 ggcatcccac cggaagtacc gcgaagagtc cgtcgtcgag ctcgcggagt gggccgcgcg
     1861 cgtcgggccg tgcacgcacg cgctggtccg cgccatcctc gacgagaagc gacaccccga
     1921 gcacggcttt cgatcatgcc gtggactcaa gagcctgacg aagcgctaca cacccgagcg
     1981 aatcgaggcg gcgtgcgaac gcgcgctcgt ggtcggggcg cgctcgtacc tgaacgtcga
     2041 atcgatcctg cgccgcggtc tcgacaagca gccgctgact gaactcacgc cgagcgccga
     2101 tgagccgcct gcgctcgagc acgagaacat ccgcggcccg gactactacc actgaggaga
     2161 acatgctgaa ggaaccgacg atcgaaaagc tcaaggccat gcgtctggac gcgatggcga
     2221 gcgcgtggag cgaacaacac ggcaagcccg acgtgaaccg gctgtccttc gacgagcgct
     2281 tcgggatgct cgtcgacgcg gagtggctgc atcgcgagaa ccgccgactg acgaaggcgc
     2341 tacgcgacgc aaaactcaaa ctcgcgagtg cctgcctcga ggacatcgag tactcgtcca
     2401 agcgcgagct cgaccgcgcg cagatgcggc aactcgcgtc gtgccgctgg gtcgtcgagc
     2461 atcagaacgt tttgatcacc gggatgaccg gcacaggcaa gacgtacctc gcatgtgcgc
     2521 tcgcgcagca ggcgtgccgg aagggacatc gcgcgatata tcgtcgcgcg tcgcggctct
     2581 tctcggagct cgcgctcgct cgcgccgacg gcacgtacgc tcggctactg acgcgcttcg
     2641 cccgtgctga cgtcctcgtc cttgacgact gggccatggc accggtcacc gattccgagc
     2701 gccgtgacct cctcgaggtc ctcgaggatc gctacggcac acgatcgacg atcgtgacga
     2761 gccaggtgcc gccggagaag tggcacaaac acatcgccga cccgactcac gcggacgcaa
     2821 tctgcgaccg gctgatccac aacgcccaca agctcgtgct aaaggggccg tccaagcgaa
     2881 agggcgagga ggccgagacc gagaagtaac ccacgatcac cgactccatc gccctgcgat
     2941 cgccatcgac cggtttcggc gatcacgatg cgccggtttc ggtgatcgcg atgagtgaac
     3001 tacgcagctc ggggcgtgcc gcacgctcaa cggcgagacc gactcgatgt cgacaatgga
     3061 cacgaggccg cgtcgcgttc tgccaaatcc ccaatagaca attcttcccg ggccgccgtc
     3121 cgcgatcgat tcgacgctcg ctccgcgccg ccgatcgcga tctagaaaac tagaagtcgc
     3181 ccgggaggat tctgtcgagc ggcagcgctt cgcgatgcgg cggtcgtgcc tgctggcccg
     3241 ggaggcccac caatcgtgcg caacgcgctc gtcctccggc acgagaaaga cgagtcgcat
     3301 aaaccgagac gtgactcctg caggcgcgtg atcaatttcg gcgatcgccc cctgcaggcg
     3361 ggatcaaatt cctttgcgcc gccgggtcgg gccgtcgact ccgagcgtct gggtgccttt
     3421 tctttcgcgt ccgacgtggg atgcgtcata gggacggctt tcaaagcaaa t
//
LOCUS       DATLBZ010000549        15985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2956578, whole genome shotgun sequence.
ACCESSION   DATLBZ010000549 DATLBZ010000000
VERSION     DATLBZ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15985
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2956578"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VMZ53_27565"
     CDS             complement(<1..595)
                     /locus_tag="VMZ53_27565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32302.1"
                     /translation="MRPIDWLLAHLDEPGVAEIVIGVGRPVAVRGAEGFRAVTSMNFE
                     RNDIETLVAGTPMSGLIGSPTSLPPAELQLDARRLVVEVVRQGYNAALRIALVGAPPV
                     DAEPRPASTTSAPRTATPTSAPRTTTPTSAPSRTSTPTSAPHAATPTSTPDPMTSYAP
                     PRTSTPTSAPDTMTSYAAPRTSTPTAAPSRTSTPTAAP"
     gene            703..1695
                     /locus_tag="VMZ53_27570"
     CDS             703..1695
                     /locus_tag="VMZ53_27570"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HUS32303.1"
                     /translation="MAFDPLPYLAPLFVVALVVEGVLVGYSRRALISGIGCIVIDQVA
                     AAWGLALFVSAFAVLHSEIAVVELSRSSIKTWVIAIVAHDLAYYWFHRISHRVTVLWA
                     AHVVHHQSERYDFTVSLRQGSVATWISYAFYAPLAILIDAQVFLIVHAAYQLYQFFVH
                     TRAIGRLGPLEWVFATPSHHRVHHGSERRHLDRNYGGFFIIFDRLFGTFTPEDREPRY
                     GVPGGYAMTSPLFANTYMFLRLLAASPGLSLWQRITLWFGPPEDTARDVSSAAHPPGV
                     VETAPFAATWLPLVTAVACAMGVAFVDLPTTITVALAIGSVVLVEIASGPLDGR"
     gene            1965..2774
                     /locus_tag="VMZ53_27575"
     CDS             1965..2774
                     /locus_tag="VMZ53_27575"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HUS32304.1"
                     /translation="MIAIESTVSALNSSGFAALKGAFSRDWALRMREDIDVAFREARA
                     RKGGAVERGPNRFYVEIHPEQFRGYVDLVTHPWVAGVCRAVLGPDYEIVELGFDVPLP
                     GAQNQPWHRDFASPKETHEGRRLTSLAFNLSGVDTTLEMGPFEIAPGTQWEAGTEFRS
                     SMFPPESEWPRYAAIAQQKLAQLGDISVRSALTIHRGTANRSQHARPVLVLGVDAPGA
                     GHAALHDQLVTRAGYEAFPALVRKHLVCRVVDQLVPIVQKHTIEGLLVAAP"
     gene            2876..3712
                     /locus_tag="VMZ53_27580"
     CDS             2876..3712
                     /locus_tag="VMZ53_27580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32305.1"
                     /translation="MRSLEFAGHAAVELAAGPARMVVVHDVGPRIAWFGLVGRENLLY
                     WDTAGAHREGAWRLYGGHRLWVTRPLADESAEIHEPDNAPCVVEELEDGVRIRAPRTT
                     LGLEKTLEVRVRGAEWSVRHHLRNSSGLLWSGGAWALTCTLPSAETVYRIPLGGGPPT
                     WDIATIIVPLSWGTHTSRLDDPQISFNGRVMEVRAQSDESKRMVRAPLGTIEMHDAAR
                     GLFRKVSPFDQNGAYPLDANLALYVGPQRFMVEMESMSALRTLAPGMTLEHVEVWTIE
                     SL"
     gene            3748..4929
                     /locus_tag="VMZ53_27585"
     CDS             3748..4929
                     /locus_tag="VMZ53_27585"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HUS32306.1"
                     /translation="MRSVPNAVLCLSHLRWKFVYQRPQHLLSRCARERRVIFFEEPLY
                     DGGETELELTETPEGVLTIVPHLAPGTSFERAEAVQRKMIDHVLEELAVPEPILWYYT
                     PMALGFTDHVNAAAVVYDCMDELSMFQGAPPELVQRERRLLERADVVFTGGNSLYEYK
                     RGAHHNIHPFPSSVDVDHFARARELVEDPADQEMIAHPRVGFFGVIDERMNLGLLSEL
                     ADMRPDLQLVMIGPVVKIDPSTLPRRDNIHWLGSKTYAELPSYLAGWDVAMLPFAIND
                     STRFISPTKTPEYLAAGCPVVSTPIRDVVEPYGRQGLAWIANTETEFSVAIDEALASD
                     QTARRAQADAFLSELSWDKTWGAMWAHVERAMAKNEAPPLFRIGTGTGATQHALSKRE
                     E"
     gene            4933..6090
                     /gene="glf"
                     /locus_tag="VMZ53_27590"
     CDS             4933..6090
                     /gene="glf"
                     /locus_tag="VMZ53_27590"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HUS32307.1"
                     /translation="MFDYLIVGAGFAGSVLAERLAVGSGKRVLICDKRPHVAGNAYDC
                     YDDAGVLVHKYGPHIFHTNSAPVFEYLSRFTAWRQYEHRVLASVDGQLVPIPINLDTV
                     NRLYGLTLTSFEVEAFFQKVAEKIDHVRTSEDVVVSKVGRELYEKFFRGYTRKQWGLD
                     PSELDASVTARVPTRTNRDDRYFTDTYQVMPMHGYTRMFERMLAHENIKVMLNTDYRE
                     IERLIPYREMIYTGPVDAFYDYTYGKLPYRSLEFRHETLTESRVQSVGTVNYPNEHAY
                     TRITEFKHLTGQEHSKTSVVYEYPRAEGDPYYPVPRPENAALYERYRELTKATPRVHF
                     VGRLATYRYYNMDQVVAQALKLYADLTEEHANGRSIEHAIARNVGGDRVHG"
     gene            6047..7270
                     /locus_tag="VMZ53_27595"
     CDS             6047..7270
                     /locus_tag="VMZ53_27595"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HUS32308.1"
                     /translation="MPSLAMWAGIECTVNRVGDRYFDQLVRTGHHDRTRDLDLIAELG
                     IRTVRYPVIWERIAPRTIAEADWSWTDERLGRLRELGISPIVTLVHHGSGPAYTSLLD
                     DRFPELLAEYARAVARRYPWITMFTPVNEPLTTARFSALYGHWYPHARDHASFVRAVM
                     NQCKAITLAMRAIREEIPNAKLVTTEDLGVTRGVPELQYQIDRENERRWASMDLLCGQ
                     RRSVLGADATWFVENPGPPDIMGFNYYLTSERWLDTNVAAWPAWSHGGNGVDRYADIH
                     AVLARKMRGIAPLLAQAWQRFGVPLAITEAHLGGTREEQMRWLLDIYDAANALVARGI
                     PIRAMTIWSLFGCVDWNCLVTNDNGFYEPGAFDIRGPAPRPTALARIVSELAAGRRPA
                     HPVLAGTGFWRRHAS"
     gene            7260..9089
                     /locus_tag="VMZ53_27600"
     CDS             7260..9089
                     /locus_tag="VMZ53_27600"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HUS32309.1"
                     /translation="MRAKWMPRDLDRWIARGEHPRPQMQRESWRSLDGTWDFAFDDDA
                     RWSQPHDVRFDRTIRVPYPPESDASGIGDQRFHHVCWYRTSIALTPIERRGRVLVHFG
                     SVDYRSTVWANGQLVCEHSGGHTPFSADITHVVRDGERVELVLRAADDPFDLAQPRGK
                     QDWLEDPHEIWYPRTTGIWQTVWIETVPTTSIRNLVWTPYLDQWEMGIGVELEGPVEP
                     GTTVRVRLAHAGQILADDRYLANGREIVRRIAFSDPGIDDYRNHLLWSPLHPLLIDAV
                     VELWQGDRCVDAVWSYTALRSVGVQGNRFMLNGRPFFLKMVLDQGYWPDTLMSPPNAT
                     ALRQDVEATIALGFNGVRKHQKLEDPRWLFFCDLYGLLVWEEMPSPYRFTGNAVQRLI
                     AEWTEAIRRDVSHPCICAWVPLNESWGVPDLTTNPAHRDYVRALYHLTKTLDPTRPAV
                     GNDGWEHVATDFVTIHDYASNPAVLRERYATPESVELVLQRQEPGGRAITLAGHSPQE
                     HPVILSEFGGIALIRDDSEHGWGYSRVRDPEQLASAYEGMLRALHACRGLAGFCYTQL
                     TDTFQERNGLLTATRRPKVEVARIAAATRGNRKAFAMDVDPEI"
     gene            9092..10195
                     /gene="galT"
                     /locus_tag="VMZ53_27605"
     CDS             9092..10195
                     /gene="galT"
                     /locus_tag="VMZ53_27605"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HUS32310.1"
                     /translation="MAMPKAVHPLHRRVLHKPDGRELILYGRTPLDPTVVAPQAGAAG
                     QRTSHLRWHPLRGEWVVYAGHRQDRTFLPPPGWNPLASSTDPAHPTEVPAGNWQVAVF
                     ENLFSALGTQVPPPPELVATAPCGGRTEVVVFTQNPQGSLGGLSLDQLALVVDVWAER
                     TTVLGGLDDVAHVFPFENRGVEVGVTLQHPHGQIYAYPFVPPIIARELELMRAHTAEH
                     GRGLLADLIAAEIAARARTLYVGPHALAWVPVCARWAYEIWIAPRRAAATIDALDDQE
                     RWDLARALQICLRKLDKLWDRPMPYLLALHQAPSRGTHPYAHVHIEIYPWLRMPGRLK
                     YLAGSEIGAGVFTADTLPEMKAQELRAVQVDHD"
     gene            10188..11252
                     /gene="galK"
                     /locus_tag="VMZ53_27610"
     CDS             10188..11252
                     /gene="galK"
                     /locus_tag="VMZ53_27610"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HUS32311.1"
                     /translation="MISASAPGRVNLIGEHTDYNGGLVLPTPIPQRTRVELELRDDAR
                     VIVSTAELATSAEFVLGNERRSGTWLDYVQGCTAAARRAGFPLHGANIAISSNVPLGS
                     GLSSSAALEVAVLRAFREALHLDLDDLALALLGQRAENDLVGAPVGAMDQVAVSMGTP
                     GAALLIDMRTLAARRIPLPHADLLVISSGIQHDHAAGDYRTRRAECEEAARLLGVKLL
                     SDLDVGDLARICRLPTPLRGRVRHVVTENARVRQAVEAIEQNDLVRLGVLFRESHKSM
                     RDDYEVSIAAIDMLVEIAEAQPDVYGARLTGGGFGGSIVAIAKPGSGERAADAIIHEF
                     ARTGYSGQVLIAGEVPCDPS"
     gene            11237..12073
                     /locus_tag="VMZ53_27615"
     CDS             11237..12073
                     /locus_tag="VMZ53_27615"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS32312.1"
                     /translation="MRPILITGASGTLGRAFARICDERGLAYHVTSRSELDIATEDHA
                     ERALDRYEPWLVVNAAGYVRVDDAEHDAERCHRENALGPANLARACAHAGVQLVSFSS
                     DLVFDGTKGEPYEEHDTPRPLGVYGRTKYEAECRVLDAHSQALVVRTSAFFGPWDEHN
                     FVTQTLATLASGRSVHAAIDATVSPTYVPDLVHTCLDLAIDAESGIWHLANQGATSWA
                     MLAQTAARLHGYDPERIIGVATDTLSLRARRPLFSALGSARGLLLPHHEDALGRYQGA
                     RT"
     gene            12082..13086
                     /gene="galE"
                     /locus_tag="VMZ53_27620"
     CDS             12082..13086
                     /gene="galE"
                     /locus_tag="VMZ53_27620"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HUS32313.1"
                     /translation="MHGSTLLVTGGAGFVGSHFVHMAAEAGREVIVLDDLSGGEPALL
                     PSSVRLIVGDIGERAYLTRLLRDNHVGAIVHFAGKIQVGESVRDPAGYFDVNLVRTLE
                     LLHAVRDAEVGVCVFSSTAAVYGEPEVVPIPELARREPVNAYGATKLCVELALAAWGE
                     AYGLRWAALRYFNAAGARPDGTLRESHAPETHLIPLALDAGLGTAPALNVFGTDYPTP
                     DGTCVRDYIHVQDLASAHLAALAKLEAGQQLGAMNLGTGHGYSVREVLDAAGHVLGRP
                     VPHSTVARRAGDPPQLVADPTRAMNVLGWQPRRSTLELIVEDALRPRIGVARARSKIA
                     "
     gene            13215..13580
                     /locus_tag="VMZ53_27625"
     CDS             13215..13580
                     /locus_tag="VMZ53_27625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS32314.1"
                     /translation="MLRVLIVDDYPETANILASLVESLGHDCRTAGTGRHALAEALAF
                     EPHVTLLDLGLPDLSGFDVARALRAQPGGATRRIIALTGWATDRDRKLAHQAGCDDFL
                     LKPATSKKLQTLLRVNAKG"
     gene            complement(14561..>15985)
                     /locus_tag="VMZ53_27630"
     CDS             complement(14561..>15985)
                     /locus_tag="VMZ53_27630"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HUS32315.1"
                     /translation="GGSMRAEGIRDEGLSGGSMRAEGIRDEHLSGGSMRVERIRGERL
                     SGGSVRAQGIRVERLSGGSMRAEGIRGERLSGGAVRAGGVRDKRLSGARLARVVAISL
                     LALGLAVTRRAEAHPLDLGYLRITTTGDRTAIQLDLDIDAAAKLIGVDARTLDTRTVQ
                     ALAEELARKSFERQPIMTATGECSFTRAAAALSGRTVSAWATAECPAAGEGAQASGGP
                     RRWELPMVADGVISSRFPLMVKRVVGGSERLAIVDKHSSSLVLAPADETTSGGGGASG
                     GVGFFAFVRDGIEHIGAAPNQWRDDSGGWKLPDGIDHILFLLALLLAGGRLSRLVGIA
                     SGFTLGHSITLALAALDIVRLPASIIEPLIALSIAFAAAEAFTSKLERHRWKIATGFG
                     FIHGFGFASALSHLALSTRGKVTALFGFNLGVELGQLVIVLLAAPLILALHRHKRIGT
                     PVLRVAAGVIFVCGLYWFVVRAFG"
BASE COUNT         2778 a         5272 c         5155 g         2780 t
ORIGIN      
        1 aaggcgcggc tgtcggcgtc gacgtccgag aaggcgccgc cgtcggcgtc gatgtccgag
       61 gcgccgcgta cgacgtcatc gtgtctggcg cggacgtcgg cgtcgatgtc cgaggcggcg
      121 cgtatgacgt catcgggtcc ggcgtggacg ttggtgtcgc ggcgtgaggc gcggatgttg
      181 gagtcgatgt ccgagaaggc gcggacgtgg gcgtcgtcgt gcgaggcgcg gacgtcggag
      241 tcgccgtgcg cggcgccgac gtcgtgctcg ctggccgcgg ctcggcatcg acgggcggcg
      301 cgccgaccag cgcgatccgc agcgccgcgt tgtagccttg gcggacgacc tcgacgacga
      361 gacggcgcgc atcgagctgc agctcggcgg gcggcagcga cgtcggcgat ccgatcaagc
      421 ccgacatcgg ggtccccgcg acgagcgtct cgatgtcgtt gcgctcgaag ttcatcgacg
      481 tcaccgcgcg aaagccttca gcgcctcgca cggcaaccgg ccgccccacc ccgatgacga
      541 tctcggcgac gcccggctcg tcgaggtgcg ctagcaacca gtcaatcggt ctcatgtcgt
      601 ccccttccgc ccgctacccc aagaataacc gtgcggcgcg cgcttcctca atgaccttcg
      661 tggcggcgaa ttggggattc agcgtctaca gtcgagcccc tgatggcctt cgacccgctg
      721 ccttatctgg cgccgctgtt tgtcgtggca ttggtcgtcg agggagtcct cgtcgggtat
      781 tcgcgacggg cgctgatttc gggcattggc tgcatcgtga tcgaccaggt ggcggcggcg
      841 tggggcctag cgctgtttgt gagcgcattc gcggtgctgc atagcgagat cgcggtcgtc
      901 gagctcagcc gatcgtcgat caagacgtgg gtcatcgcga tcgtcgcgca tgacctcgcg
      961 tattactggt tccaccgcat ctcgcatcgc gtgaccgtgc tgtgggcggc gcatgtcgtt
     1021 catcatcaga gcgagcgcta tgacttcacc gtgtcgctgc gccagggctc ggtcgcgacg
     1081 tggatcagct atgcgttcta cgcaccgctc gcgattctca tcgacgcgca ggtgttcttg
     1141 atcgtgcatg cggcgtatca gctctatcaa ttcttcgtgc atacgcgcgc gatcggccgg
     1201 ctcggcccgc tcgagtgggt gttcgcaacg ccgagtcatc atcgtgttca ccacgggtcc
     1261 gagcggcgtc acctcgatcg caactacggc ggcttcttca tcatcttcga tcggttgttc
     1321 ggcacgttca cgcccgagga tcgcgagccg cgttatggag tgcccggtgg ctacgcgatg
     1381 acgagcccgc tgttcgcgaa cacgtacatg ttcctgcgac ttcttgccgc gagccctggc
     1441 ctctctctgt ggcagcgcat cacgctgtgg tttggtccac cggaggacac ggcgcgcgat
     1501 gtgtcgtcgg cggcgcaccc tccgggcgtc gtcgagactg cgccgtttgc ggcgacctgg
     1561 ctcccgctcg tgaccgcggt tgcatgcgcg atgggtgtgg catttgtcga tctgccgacg
     1621 acaattaccg tcgcactcgc gatcggtagt gtcgtgctcg tcgaaatcgc gagcggtccg
     1681 ctcgacggcc gctgagctca attaacgggc gtcgtatcta cgggcggagg tgttcttcgg
     1741 cggctcacga gacggtcgac gtgctcgatc aagagcccga cctcgtcctg gagtgtttcg
     1801 agtttctccg tcacgccacg acgcgtgcgt cgggaggcga acagcctgcg aaggaatccc
     1861 atggttcttc gcgctgcaac gacgagacca ccgcacgacg caaccgattt tctgagatcg
     1921 cgcgagcagc ttgcgttgtg acgacgtgcc gctagcctgc gcgcatgatc gcgatcgaat
     1981 cgacggtgag cgctctgaac agctcggggt ttgctgcatt gaagggagcg ttctcgcgtg
     2041 actgggcgtt gcggatgcgc gaggacatcg acgtcgcgtt tcgcgaggcg cgcgcgcgga
     2101 agggtggcgc cgtcgagcga ggcccgaacc ggttctatgt ggagattcac ccagagcagt
     2161 tccgcggcta cgtcgatcta gtcacgcatc cgtgggtggc cggcgtgtgc cgcgcggttc
     2221 tcggccccga ttacgaaatt gtcgagctcg ggttcgatgt gccgctgcct ggcgcgcaaa
     2281 atcaaccgtg gcaccgcgac tttgcatcgc cgaaggaaac gcacgagggc cggcgcctca
     2341 cgtcactcgc gttcaatctc agcggcgtcg acacgacact agagatgggg ccattcgaga
     2401 ttgcgcccgg aactcagtgg gaggccggga cggagtttcg gagctcgatg tttccaccgg
     2461 agtccgagtg gccgcgctac gcagcgatcg cgcagcaaaa gctcgcgcag ctcggcgaca
     2521 tctcggtgcg ctccgcgctg acgattcatc gaggcacagc caatcgctcg caacacgcgc
     2581 gtcccgtgct cgtgctcggc gtcgatgcgc ctggcgcagg tcatgcggcg ctccacgatc
     2641 agctcgtcac gcgtgcggga tacgaagcat ttcccgcgct cgttcgcaag cacctcgtct
     2701 gtcgcgtcgt cgaccagctg gtgccgatcg tgcagaaaca cacgatcgaa ggtctcttgg
     2761 tcgcggcgcc ctgatcagcc cgcgatcagc ccgcgaccag atcgtgcgga tacgcgcaga
     2821 acaatcgact cgcgcgccga gcggtaccgg cacgatcctg gcactcgagc ggaacgtgcg
     2881 ctcgctggaa tttgcaggtc acgccgcggt cgagctcgct gcgggtcctg cgcgaatggt
     2941 agtcgttcac gacgtcggac cacgaatcgc gtggttcggt ctcgtcgggc gcgagaatct
     3001 tctttactgg gacaccgccg gcgcgcatcg cgagggtgcg tggcgactct acggaggcca
     3061 tcgactgtgg gtcacgcggc cgcttgcgga cgagagtgcc gagatccacg agccagacaa
     3121 cgcgccctgc gtcgtcgagg agctcgagga cggcgtccgc attcgcgcgc ctcggacaac
     3181 gctcggtctc gagaagacgc tcgaggttcg cgtgcgtggt gcggagtggt ccgtacggca
     3241 ccacctacgc aactcgagtg ggctcttatg gtccggcggc gcatgggctc tgacgtgcac
     3301 actgccgagc gccgagaccg tgtatcgaat cccgctgggc ggaggtcccc cgacatggga
     3361 tatcgcaacg atcatcgttc cgttgtcttg gggcacgcac acgtcgcgtc tcgatgaccc
     3421 gcagatctcg ttcaacggcc gcgtgatgga ggttcgtgcg cagtcggacg agagcaaacg
     3481 gatggtgcgt gcaccactcg ggacgatcga gatgcacgac gcggcgcgcg gcttgttccg
     3541 caaggtctca ccgttcgatc aaaacggtgc gtatccgctg gacgccaatc tcgcattgta
     3601 cgttggacca cagcgcttca tggttgaaat ggaatcgatg agcgcactcc gaacgctcgc
     3661 gccggggatg acactcgaac acgtcgaagt ctggacgatc gagagtctct gaggtccgcg
     3721 gcccggaccg tgcacttctc gcttgtcatg cgaagcgtcc cgaatgcagt cctctgtctg
     3781 tcgcatctgc gttggaagtt cgtgtaccag cgtccgcagc atctgctcag tcgatgcgcg
     3841 cgcgaacgcc gcgtgatctt cttcgaagaa ccactttacg atggtggcga gacggagctg
     3901 gagctgacgg agacgcccga aggcgtgctc acgatcgttc cgcacctcgc acccggcacc
     3961 tcgttcgaac gcgccgaagc agtgcagcga aagatgatcg accacgtgct cgaggagctc
     4021 gcggttcccg aaccgatcct ctggtactac acgccgatgg cgctcgggtt cacggatcac
     4081 gtgaacgccg ccgcagttgt ttacgactgc atggacgagc tctcgatgtt tcagggcgca
     4141 ccacccgagc tcgtgcagcg cgaacgacgg cttctggaac gcgctgacgt cgtgttcacc
     4201 gggggcaaca gcctctacga gtacaagcgg ggcgcacatc acaacatcca tccgttccct
     4261 tcgagcgtcg atgtcgacca cttcgcgcgt gctcgcgagc tcgtcgaaga cccggccgac
     4321 caagagatga tcgcgcatcc gcgcgttggc ttcttcggcg tgatcgacga acgaatgaat
     4381 ctcgggctgc tcagcgagct cgccgatatg cggccggatc tgcagctcgt gatgatcggc
     4441 cccgtcgtga agatcgatcc ttcgacgctg cctcgtcgcg acaacatcca ctggctcggc
     4501 tcgaagacgt acgccgagct gcctagctac ctcgccggct gggacgtcgc gatgctgccg
     4561 ttcgcgatca atgactcgac gcgcttcatc tcgccgacga agacgcccga atatctggcg
     4621 gcgggctgtc ccgtcgtctc cacgccgatc cgtgacgtcg tcgagccgta cggccggcaa
     4681 ggcctcgcgt ggatcgcgaa caccgaaacc gaattctcag tcgcgatcga cgaagctctc
     4741 gcctccgatc agacagcgag acgcgcgcaa gcggacgcct tcctctccga gctgtcgtgg
     4801 gacaagacgt ggggggcgat gtgggctcac gtcgagcgcg cgatggcgaa gaacgaagca
     4861 cctcccctgt tccgaatcgg cactggcacg ggtgcgaccc agcatgcgct ttcaaagcgc
     4921 gaggagtagc caatgttcga ctacttgatc gtcggtgctg gcttcgccgg ctccgtgctc
     4981 gcggagcgct tggccgtcgg ttctggcaag cgagtcctga tttgcgacaa gcgtccccac
     5041 gtcgctggca atgcctacga ttgttacgac gacgcaggcg tgctcgtgca caagtacggg
     5101 ccgcacatct ttcacaccaa cagcgcaccc gtgttcgagt acctgtctcg cttcacggcg
     5161 tggcggcagt acgagcaccg cgtgctcgca tcggtagacg gtcagctcgt cccgatcccg
     5221 atcaacctcg acacggtcaa tcggctgtac gggttgacgc tgacgtcgtt cgaagtcgaa
     5281 gccttcttcc agaaggtcgc ggagaagatc gatcacgtcc gcacctcgga agacgttgtc
     5341 gtcagcaaag tcgggcgaga gctctacgag aagttttttc gcggttacac gcgcaaacag
     5401 tggggcctcg atccaagcga gctcgatgcg tcggtgacag cgcgagttcc gacgcgcacg
     5461 aaccgcgacg accgctactt caccgacacg tatcaagtga tgccgatgca cggctacacg
     5521 cggatgttcg agcggatgct cgcgcacgag aacatcaagg tgatgctcaa cacggactat
     5581 cgcgagatcg agcgactcat cccgtaccgc gagatgatct acaccgggcc ggtcgacgcg
     5641 ttctacgact acacgtacgg gaagttgccg taccgctcgc tcgagttccg ccacgagacg
     5701 ctgaccgagt cgcgcgtgca gtccgtcggg accgtgaact atcccaacga gcacgcgtac
     5761 acgcgcatca ccgagttcaa gcacctcacc ggccaggagc acagcaagac gagcgtcgtc
     5821 tacgagtatc cgcgggccga aggcgatccg tactatccgg tgccgcgccc cgaaaatgcg
     5881 gcgctgtacg agcgctatcg cgagctaacg aaggcgacgc cgcgcgttca cttcgtcgga
     5941 aggctcgcga cgtaccgcta ctacaacatg gaccaagtcg tcgcgcaggc actcaagctg
     6001 tacgccgatc tgacagagga gcacgcgaat ggtcgatcga tcgagcatgc catcgctcgc
     6061 aatgtgggcg gggatcgagt gcacggttaa ccgcgttggt gaccggtact tcgaccagct
     6121 cgtgcgtacg ggccatcacg accgcacgcg cgatctcgac ctgatcgcgg agctcggaat
     6181 ccgcacggtg cgctatcccg tcatctggga gcgcatcgcg ccgcgcacca tcgcggaagc
     6241 ggactggtcg tggacagacg agcgactcgg gcgcctgcgc gagctcggca tctcgccgat
     6301 cgtcacgctc gtccatcacg gctcgggtcc ggcatacacg agcctcctcg acgatcgctt
     6361 ccccgagctg ctcgccgagt acgcgcgtgc ggtcgcgcgt cgctatccgt ggatcaccat
     6421 gttcaccccc gtcaacgagc cgctgaccac ggcgcggttc tcggcgctct acggccattg
     6481 gtatccgcat gcgcgcgatc acgcgtcgtt cgtgcgtgcg gtaatgaatc agtgcaaggc
     6541 gatcacgctc gcgatgcggg ccatccgtga agagattcca aacgcgaagc tcgtcacgac
     6601 cgaggacctt ggcgttacgc gcggcgttcc cgagctgcag tatcagatcg atcgcgagaa
     6661 cgaacgtagg tgggcgagca tggatctgct gtgcggccaa cgccgctccg tgctcggcgc
     6721 cgacgcgacc tggttcgtcg agaacccggg cccgcccgac atcatgggct tcaactatta
     6781 cctcacgagc gaacgctggc tcgacaccaa cgtcgccgcg tggcccgcct ggtcgcacgg
     6841 cggcaatggc gtcgatcgtt acgcggacat ccatgccgtg ctcgctcgca agatgcgagg
     6901 catcgcgccg ctgctcgcac aggcgtggca gcgcttcggc gtcccgctcg cgatcaccga
     6961 ggcgcacctc ggcggcaccc gcgaggaaca gatgcggtgg ctcctcgata tctacgacgc
     7021 ggcgaacgcg ctcgtggcac gaggcattcc gatccgcgcg atgacgatct ggtcgctgtt
     7081 cggctgcgtc gactggaact gcctcgtcac gaacgacaac ggcttctacg aacctggcgc
     7141 gttcgacatc cgcggtccgg cgccgcgtcc gaccgcactc gcacgcatcg tctcggagct
     7201 tgctgcaggc cgccggccgg cacaccccgt acttgccggc actggcttct ggaggcgtca
     7261 tgcgagctaa atggatgcca cgcgatctcg atcgctggat cgcgcgcggc gagcacccgc
     7321 gcccgcagat gcaacgcgag agctggcggt cgctcgacgg cacgtgggac ttcgcgttcg
     7381 acgatgacgc acgctggagc cagccgcacg acgtacgctt cgatcgcacg atccgcgtgc
     7441 cgtatccacc ggagagcgac gcgagcggaa tcggcgacca gcgctttcac cacgtgtgct
     7501 ggtaccgcac gagcattgct ctgacgccaa tcgagcgtcg gggtcgcgtc ctcgtccatt
     7561 tcggcagcgt cgactatcgg tcaacggtgt gggcgaatgg ccaactcgtg tgcgagcaca
     7621 gtggaggtca cacgccgttc tccgcggaca tcacgcatgt cgttcgcgac ggcgagcgcg
     7681 tcgagctcgt gcttcgtgcg gccgacgatc cgttcgatct cgcccagccg cgcggcaaac
     7741 aagactggct cgaggacccg cacgagatct ggtatccgcg cacgaccggc atctggcaga
     7801 cggtgtggat cgagaccgtg ccgacgacgt cgatccgaaa cctcgtgtgg acgccgtacc
     7861 tcgatcagtg ggagatggga atcggcgtcg agctcgaggg tccggtggaa ccaggaacaa
     7921 cggtccgtgt gcgcctcgcc catgccggcc agatcctcgc ggacgatcgc tacttggcga
     7981 atggccggga gatcgtgcgc cggatcgcgt tctcggatcc gggcatcgat gactaccgca
     8041 accatctgtt gtggagccca ctgcatcccc tgctgatcga tgcggttgtc gagctgtggc
     8101 aaggcgaccg ctgcgtcgat gcggtttgga gctacaccgc gctgcgttcc gtcggcgtgc
     8161 aaggcaatcg cttcatgctc aacggccggc ccttctttct gaagatggtg ctcgaccagg
     8221 gttactggcc agacacgctg atgtcgccac cgaatgcgac ggcgcttcgc caagacgtcg
     8281 aggcgacgat cgcgctcggc ttcaacggcg tgcgcaaaca tcagaagctc gaggatccgc
     8341 gctggctgtt cttctgcgac ctctacgggc tgctcgtgtg ggaagagatg ccgagcccgt
     8401 atcgcttcac gggcaacgcg gtgcaacggc tgatcgcgga gtggacggag gccatccgtc
     8461 gtgatgtctc gcatccgtgc atctgcgcgt gggtgccgct caacgagtcg tggggcgtac
     8521 ctgatctgac gacgaacccg gcgcatcgcg actacgtgcg cgctctgtat cacctcacga
     8581 agacgctcga tccgacacga cctgcggtgg gtaatgacgg ctgggagcac gtcgcgaccg
     8641 acttcgtcac gatccacgac tacgcatcga accccgcggt gcttcgcgaa cgctacgcga
     8701 cgccggagtc cgtcgagctg gtgttgcaac gccaggagcc gggcggacgc gcgatcacgc
     8761 tcgcgggtca tagcccacaa gagcatccgg tgatcctctc cgagttcggc ggtatcgctc
     8821 tcatccgcga tgacagcgag catggctggg gctacagccg cgtgcgcgat cccgaacaac
     8881 tcgcgtccgc gtacgagggc atgctgcgcg cgctccacgc gtgtcgtggc ctcgccggtt
     8941 tttgctacac gcaactcacg gacacgttcc aggagcgcaa cgggctgctc accgcaacac
     9001 gtcgcccgaa agtcgaggtt gcgcgaatcg cggccgccac tcgcggtaat cgcaaagcgt
     9061 tcgcgatgga tgtcgatccg gagatctgac catggcaatg cccaaagctg tccatccact
     9121 gcatcggcgc gtgttgcaca agcccgatgg tcgcgagctg atcctctatg ggcgcacgcc
     9181 gctcgatccc acagtggtcg cgccacaagc gggcgctgca ggtcaacgca catcgcatct
     9241 tcgctggcat ccgctgcgcg gcgaatgggt cgtctacgcg ggccaccggc aggaccgaac
     9301 gttcttacct ccgccgggat ggaacccgct ggcttcatcg acggatccgg ctcatccgac
     9361 ggaggttccg gcaggcaatt ggcaggtcgc tgtcttcgag aatctgttct ccgcgctcgg
     9421 aacgcaggtg ccaccaccgc ccgagctggt cgcgacagca ccttgcggag gtcgcacgga
     9481 ggtcgtcgtc ttcacccaga acccgcaagg ctccctcggt ggactgtccc tcgatcagct
     9541 cgcactcgtc gtcgatgtct gggccgagcg cacgaccgtg ctcggcgggc tcgacgatgt
     9601 cgcgcacgtc ttcccattcg agaatcgcgg cgtcgaagtc ggagtcacgc tgcaacatcc
     9661 gcacgggcag atctacgcgt atccgttcgt gccaccgatc atcgcgcgcg agctcgagct
     9721 gatgcgcgcg cataccgccg agcacggccg tggcttgctc gctgacctga tcgcggcaga
     9781 gatcgcggcg cgcgcgcgca cgttgtacgt cggcccgcat gcgctcgcgt gggttcccgt
     9841 gtgtgcgcgt tgggcgtacg aaatctggat cgcgccgcgg cgggccgcag caacgatcga
     9901 tgcgctcgac gaccaggagc gctgggatct cgcacgagcg ctgcaaatct gtttgcgcaa
     9961 gctcgacaag ctgtgggacc gcccgatgcc gtacttgctc gcgctccatc aagctccgtc
    10021 gcgcggcacg catccatacg cgcacgtgca catcgagatc tatccgtggc tgcgaatgcc
    10081 gggacgtctg aagtatctcg ctggcagcga gatcggcgcc ggcgtgttca ccgctgacac
    10141 gctgcccgag atgaaagcac aggagcttcg agccgtgcag gtggaccatg attagcgcgt
    10201 cggcgcctgg ccgcgtcaat ctcattggcg agcacaccga ctacaacgga gggctcgtgc
    10261 tgccgacgcc aatcccgcag cggacacgcg tcgagctcga gcttcgcgac gatgcacgtg
    10321 tcatcgtgtc gaccgcagag ctcgcgacct cggcggagtt cgtgctcggc aacgagcgac
    10381 gcagcggcac gtggctcgac tacgtgcagg gctgtaccgc cgcggcgcgg agagctggct
    10441 tcccgctaca cggcgcaaac atcgcgatct cgtcgaacgt gccgctcggc agcgggctgt
    10501 catcgagcgc ggcactcgaa gtggcggtgc tccgcgcatt tcgcgaagcg ctgcacctcg
    10561 acctcgacga tctcgcgctg gcactgcttg gccagcgagc agagaacgat ctggtcgggg
    10621 cacctgtcgg cgcgatggat caggtcgccg tgagcatggg gacacccggc gctgcgctct
    10681 tgatcgacat gcgcacgctg gccgcgagac gcattccgtt gccgcacgcc gatctgctcg
    10741 tgatctcgtc cggcatccag cacgatcacg cagccggcga ctatcgaacg cggcgcgcgg
    10801 agtgcgaaga ggcggcccgc ttgctgggtg tcaagctgtt atcggatctc gacgtcggcg
    10861 acctcgctcg catttgcagg ctgcccacgc cgttgcgcgg tcgtgtccgt cacgtggtga
    10921 ccgagaacgc tcgcgtccgc caggccgtcg aggccatcga gcagaacgat ctcgttcgcc
    10981 tcggtgtgtt gtttcgcgag tcgcacaaat cgatgcgcga tgactacgaa gtatccattg
    11041 cagcgatcga tatgctcgtc gagatcgctg aagcgcaacc cgacgtctac ggtgcgcgac
    11101 tcaccggcgg tgggttcggc ggttcgatcg tggcaatcgc gaagcccggc agcggagaga
    11161 gggcagccga cgcgatcatt catgagtttg cgcgcaccgg ctacagcggc caggtgctca
    11221 tcgcaggaga agtaccatgc gacccatcct gatcactgga gcgtccggaa cgcttggacg
    11281 cgcgttcgcg aggatctgtg acgaacgcgg actcgcctat cacgtcacgt cgcgcagcga
    11341 gctcgacatc gctaccgagg accacgccga acgcgcgttg gatcgctacg agccgtggct
    11401 cgtcgtcaat gccgccggtt acgtccgcgt tgacgatgcg gagcacgatg cagagcgctg
    11461 tcatcgtgag aatgcattgg gccccgcgaa tctcgcgaga gcgtgcgcgc acgccggcgt
    11521 gcagctcgtc tcgttctcgt ctgacctcgt gttcgacggc accaaaggcg agccatacga
    11581 agaacacgat acgccgcgcc cactcggcgt gtacggccgc acgaagtacg aggcggaatg
    11641 ccgcgttctc gatgcccact cgcaagcgct cgtcgtgcga acgagcgcgt tcttcgggcc
    11701 gtgggacgag cacaacttcg tcactcaaac gctcgcaaca ctagcgtcgg gcaggtccgt
    11761 ccatgcggcg atcgacgcaa cggtctcgcc aacgtacgtg cctgacctcg tccacacgtg
    11821 cctggatctc gcgatcgacg ccgagtccgg catctggcac ctcgcgaatc agggcgcgac
    11881 gagctgggca atgctcgctc agaccgccgc gcggctgcac ggatacgacc cagagcgaat
    11941 tatcggtgtc gcgacggata cgttgtcatt gcgcgcgcga aggccactct tctcggcgct
    12001 cggttcggca cgcggtctgc tcttgcctca tcacgaggac gcgcttggcc gttatcaagg
    12061 ggcgcgcacc taggaggacc catgcatggc tcaacgcttc tcgtaaccgg cggagcgggc
    12121 ttcgtcggca gccacttcgt ccacatggca gccgaagcag gacgcgaagt catcgtgctc
    12181 gatgatctgt ctggtggcga gcctgccctg ctgccgtcat cggtgcgact catcgtcggc
    12241 gatatcggtg agcgcgcgta cctcacgcgc ttactgcgcg acaaccacgt cggtgcgatc
    12301 gttcacttcg cgggcaagat ccaggtcggg gagtccgttc gcgatccggc cggatacttc
    12361 gacgtcaacc tcgtcaggac gctcgagctg ctccatgcgg tgcgcgacgc cgaggtcgga
    12421 gtgtgcgtgt tctcctcgac ggccgctgtc tacggcgagc ccgaggtcgt gccgatccca
    12481 gagctcgcac gacgcgagcc cgtgaatgcg tacggtgcaa cgaagctctg cgtggagctt
    12541 gcactcgccg cctggggcga agcatacggc ctacggtggg cggcgctacg ctacttcaat
    12601 gcggcaggcg cgcgtcccga cggcacgctt cgcgagagcc acgcgccaga gacgcacttg
    12661 atcccgctcg cactcgatgc gggcctcggc actgctccgg cgctcaacgt gttcggcacc
    12721 gactatccga cgcccgacgg gacatgcgtt cgcgattaca tccacgtgca agatctcgca
    12781 tcggcgcacc tcgccgcgct cgcgaagctc gaggcgggac aacagctcgg cgcgatgaac
    12841 cttggtacag gccacgggta ttccgttcgc gaggtgctcg acgcagccgg acatgtgctc
    12901 ggccggcccg tcccacactc cacagtcgcg cgtcgagccg gcgatcctcc gcagctcgta
    12961 gccgatccaa cacgcgcgat gaatgtgctt ggctggcaac cgcgccggtc aacgctcgag
    13021 ctcattgtcg aggacgcgct gcgcccgcga atcggcgtcg cgcgcgcgcg atcgaaaatt
    13081 gcgtagcaac ttcgtgcccc gggttcacgt tcatcgatca gaccgagcat gtcggcacgc
    13141 tccggagaag caggcgttat gcgcgatcga gatgtccttg cgagtcgcgc cgcagacgat
    13201 gtagtcgtga cttgatgctg cgtgtgctga tcgtcgacga ctatccagag acggccaaca
    13261 tcctcgcgtc cctcgtcgag tcgctcggac atgactgcag aaccgccgga accggtcgtc
    13321 atgcgctcgc ggaagctctc gcgttcgagc ctcacgtgac gttgctcgat ctcggtcttc
    13381 ctgatctgtc gggattcgac gtcgctcgcg cgctgcgagc tcagcccggc ggcgctacac
    13441 gccgaatcat cgcgctcacg ggttgggcaa ccgaccgcga tcggaagctt gcacaccaag
    13501 cgggctgcga tgatttctta ctgaagcctg caacgagcaa gaagctgcag acccttcttc
    13561 gcgtcaacgc taagggctga tcgtcgacgc gtatgccatt gccatgacga ttcggcgagc
    13621 gaccccttcg ccctcggccc agcgagtctc acgcggcccc gtcacggcga tgcgcaacga
    13681 tccggacgat gcgggaaacg accgacacca acgagcaact tctgtgctgt ctgttccgcg
    13741 gtcacagatc agcagaggca aacgttgggc acgcacgacc tgcaggactg aagcgatcgc
    13801 ggtcacgcgc tccgcagtct ctacctttgg aacgacgacg agagctccgg acgcgatcat
    13861 gaggttcgcc ctgaccgcct gacgcgggcc gcgctcgtga tgcggtgtga ggacctcgag
    13921 cacgtcggag ggaagagcac cgagctcgtc gcgcatctcc ttcgtacaaa acccagcgac
    13981 ggtcaacccg gcggcgcgtg cggcgcgatg cgcaccacgt tcgactccgg tttgcccaga
    14041 gtgaagaatt acaagcggcg ctggccagcc cgactgaaac gattgctcgt tagcgaattg
    14101 gttcgcaaca tcgctcgagc ccacgacgga aaactccacg cgacttgcca tcaacgcacg
    14161 cggtgcaagt gaagtgccct acacgtgttg cacgccgcct tagataagcg cgtcgagtcg
    14221 cttcaaatcg ggcagccggg cacagccgca attcatttcg gtcgcgcgcg agatgcgatt
    14281 cgacgaacgc gcatcaccgt gtcgctgcgt cgggctcgag atggccgatg cacttgtaga
    14341 tgagccgcag caggtcccgc cgctcggtgg cgctgcttcg gagtgtggcg tggctcgatc
    14401 acgaacgtgc gcttgtcgtc ggaagacggc acgcgccgaa cgagcccctg cgccaccatc
    14461 cgatcgatcg cgacggtgaa cgtggcgctc gactgatcgg cgatccggtg cgctggttcc
    14521 acgacgcgat cgacgcgcgc gtgggctagc ctgcgcggcg ctagccaaac gcgcgcacga
    14581 cgaaccagta caggccgcag acgaagatga cgcctgccgc gacgcgcagc acgggcgtgc
    14641 cgatgcgctt gtgacgatgc agcgccagga tcagcggcgc ggcaagcagc acgatgacga
    14701 gctggccgag ctcgacgccg aggttgaacc cgaacagtgc ggtgaccttg ccgcgcgtcg
    14761 acagtgcaag gtgcgaaagc gcgctcgcaa agccaaagcc gtgaatgaag ccgaagccgg
    14821 tcgcgatctt ccagcgatgc ctctcgagct tcgaggtaaa ggcctccgcg gcggcaaacg
    14881 cgatcgacag cgcgatgagc ggctcgatga tcgacgcggg cagccgcacg atgtcgagcg
    14941 cggccagcgc gagcgtgatc gagtggccaa gcgtgaagcc gctcgcaatg ccgacgaggc
    15001 gcgacagccg cccacccgca agcagcagcg cgagcagaaa caggatgtga tcgatgccgt
    15061 cgggcagctt ccaaccgccg ctgtcatcgc gccactgatt cggcgccgcg ccgatgtgct
    15121 cgatgccatc gcgcacgaag gcaaagaacc cgacgccacc gctcgcaccc ccgccgccag
    15181 atgtcgtttc gtcggcgggc gcgagcacga gcgacgacga atgcttgtcg acaatcgcaa
    15241 gccgctccga cccgccgacc acccgcttga ccatcagcgg aaacctcgac gagatcacac
    15301 cgtcggcgac catcggcagc tcccaccgac gaggaccgcc gcttgcttgc gcgccctccc
    15361 ccgcggccgg gcattcggca gtcgcccacg cgctgaccgt gcgaccggag agcgccgccg
    15421 cggcgcgcgt gaagctgcac tcccccgtag ccgtcatgat cggctgccgt tcgaagctct
    15481 tccgcgcgag ctcctcggcg agggcctgca cggtgcgtgt atcgagcgtt cgcgcgtcga
    15541 cgccgatgag ctttgcggcg gcgtcgatgt cgaggtcgag ttggatcgcg gtgcggtcac
    15601 cggtcgtcgt gatgcgcagg tagccaaggt cgagagggtg cgcctcggca cgccgcgtca
    15661 ccgcgagccc cagcgctagg agcgagatcg cgacaacacg tgcgaggcgc gcgcccgaga
    15721 gacgcttatc gcggactccc ccggcgcgca ccgccccgcc ggagagacgc tcgccgcgga
    15781 tcccttcggc gcgcatcgac ccgccggaga gacgctcaac gcggattccc tgggcgcgca
    15841 ccgacccgcc ggagagacgc tcgccgcgga tccgctcgac gcgcatcgac ccgccggaga
    15901 gatgctcatc gcggatcccc tcggcgcgca tcgacccgcc agagagaccc tcatcgcgga
    15961 tcccctcggc gcgcatcgac ccgcc
//
LOCUS       DATLBZ010000550        20810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3356311, whole genome shotgun sequence.
ACCESSION   DATLBZ010000550 DATLBZ010000000
VERSION     DATLBZ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20810
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3356311"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VMZ53_27635"
     CDS             complement(<1..617)
                     /locus_tag="VMZ53_27635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32316.1"
                     /translation="MRWGRQVAVATMLLLAVVHHARADALADARKAIDGSDYLSARPL
                     LETALKSGTQGPGELAEIYKLTGIVEGALGEEKGAQSAFAKWLALDPKGSLPKGTSPK
                     ITRPFDAASAKANKAGALAAKAETQDEPPAVTLVVTNDPQKMIVGARVFFMVDRKAEQ
                     RLEAEGTGTIKLELERGKRLDLRLQGIDEYGNRVVELGSHEVPIV"
     gene            complement(627..2702)
                     /locus_tag="VMZ53_27640"
     CDS             complement(627..2702)
                     /locus_tag="VMZ53_27640"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32317.1"
                     /translation="MAPEAAQASEARRFGKYTLVAKLATGGMAEIFLARLHGDGGFEK
                     LVCIKRILPHLAKNPQFVAMFLDEARVAARISHPNVCQVFELGEFQGQYYIAMEYLEG
                     VPLAVFRRKDFYPTPPQPRLVAGFAVQACEGLHHAHQLKRADGELLGVVHRDISPQNL
                     FATVDGIVKVLDFGIAKVQDQSIRTSTGAVKGTYAYMAPEQLRNEGLDRRADVWAIGA
                     VLWETLAQTHLFKRDTEFLTFEAITKLPIPHLCEVRPDVPPLMGDVIARALSRNPDDR
                     FGSARSLGEAIAQAVAPLGGPFNAAGISEEVGHSFEATLAEQRMLLRIAREGGQFDLE
                     TSSPLVGHGSAISSTPVSNIHRRAQEAQQEVAQFVAAKQAIDSLDFDDSSVRPARFGT
                     DGAFTPAPSRRSDAYASAEDFDDQETLMPMEAQKRRSAPMAAQRRSQPYEAQQDRPSG
                     PYSVAPREWRYDETEPPIPQARQTMSQPAAPIPSSRLPWFIALIAVAAAAGTGVMLYM
                     KLQQDKQKAQVVAAKEPVDEVKMPEATPPPVVVDAAVPVVEQKVEVPVVDEKPVEKPE
                     DKVADKPIEDQPPKPPIKKKTVKIDKKVETKKPEEKKPLDDKKPVEDKKPEPAVSGPP
                     GFITIDSTPVYAVIYVDGKKLGETPLVNIKLAPGKHAVRAVSPSGSARNLSITIEAGK
                     TAPVKRIEW"
     gene            complement(2718..5276)
                     /locus_tag="VMZ53_27645"
     CDS             complement(2718..5276)
                     /locus_tag="VMZ53_27645"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HUS32318.1"
                     /translation="MSRLLCLSLALASTFAAADTLPSSVRAHATRRTTPVAIDGKLDD
                     AAWAGAEKQGGFTQRFPKDGDKPTQDTRFAVLYDDDALYVGVWCADTEPDKIRRTLTR
                     RDVDALADIVVVGIDSYHDRRTGFAFQLNAAGVQRDMLLFDDSNQDDTWDAVWTGDVA
                     MVTNGWTAEFRIPLNQLRFSGAESQEWGLQVVRAIARNQEQSAWSPWPRSAPAVVSKF
                     GVIDGIEKVKPSRRLELLPYATGGLQTEPIEQGDPLNASSAALGNVGLDVKYGLGPAF
                     TLSATLNPDFGQVEADPSQVNLGANELFFAEKRPFFLEGVDLFKIQVGTGDNFAEGVF
                     YSRRIGAAPDAPDMDYDYIKQPTSTTIYSAAKLTGKTRGWSVGVLDAVTGEENAQIAL
                     GEQQMATVVSPLTNYAVARVKRDLREGHTSLGVSATAVNRALQDTPLVSTLHDQAYTA
                     GAQLSHRWDDNAWELDIHGVGSWVHGSEDAIAITQSSNRHLFQRPDASHVAFDPKRTS
                     LAGANMGWYFGRGGDTKHWRWDVGGDFRTPGLELNDAGFQLYADRNETWTWTEYRDED
                     PGKTMLNWRTSLITFMQNTFEPQIVVYGFNADIGGQLANYWSMRGAINVQGAGNDPIE
                     MRGGPALRTDPRYALHLTANSDSRKKVRVDLDFAAERKPETDYMLVRVDTGLFVQARS
                     NIDVSIGPTWTEATDPLQYVDQIDDTAGMTHYLFGRIRLTTVSLTTRMNWTFSPKLSL
                     QVYAQPFVAVGSYSEYKDVDNPHADRFEDRFKPITGGESFTFDKPDFNFGQLRSTMVL
                     RWEYRPGSALFAIWSHGQTADGTGLFDLGNDLATLARSASENIVMVKANYWIGL"
     gene            complement(5406..6497)
                     /locus_tag="VMZ53_27650"
     CDS             complement(5406..6497)
                     /locus_tag="VMZ53_27650"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HUS32319.1"
                     /translation="MANSRRGREQRLVSRCRVEFDRPSGAVEAETEDLSGRGMFIRTD
                     ALLPVGEETEIRLTLPDGTFLTFQARVAHMLTPHAAKALGRHPGMGFELIGNEAPSKL
                     KLRAYVDSIRVEITNPGLSTTTQAIVLEPGTPLRTRMTRCLEGAGFKVTAVTSMPEAL
                     DACSQWRPDVIISAAQIDDGMQGVDLAYAMSEHATLSDVPLILNGEDGDLGRLEAFRA
                     GIRDYIPRPFTDEELVIRTHRVVAPTAPVANPGLRGNLVDIGLGTLLSLFEFERKSGI
                     LLLLRDGEIARLFVSEGKVLKVEANTGNGAPKARMMSLLDWHAGQFEFSPCAIGGRDE
                     LNTSITALLLEHARVHDEQQPDKTSKVRR"
     gene            6663..7739
                     /locus_tag="VMZ53_27655"
     CDS             6663..7739
                     /locus_tag="VMZ53_27655"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HUS32320.1"
                     /translation="MTVAYEVTPVGASVLDRLASTSGARGAQDLRQRLANLGRWVRAD
                     LVEFEAELATLPKGARVVQQAAHHLLDLRGKHLRPLCVALASRFGEGFTPRARGLAVG
                     VELVHTATLLHDDVVDVAERRRGKPVAAVVYGNAASIFAGDWLLVAALRRIGAAGVDG
                     LLDKMLSVIDEMILAESLQLERRGRIVADRAEYFRIVEGKTAALFRWAMLAGARCANL
                     DAKQEAALERYGIHLGVAFQAVDDELDFAGFADAEPSEETGKDPLVDLREGKMTFPLI
                     VALEREPALHARIEAVLGDSSPNLVELTAIANAVRATGALAATRELAEQHVQLALDAL
                     ADLPAGTARESLETVALASLERQS"
     gene            7736..8536
                     /locus_tag="VMZ53_27660"
     CDS             7736..8536
                     /locus_tag="VMZ53_27660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HUS32321.1"
                     /translation="MTQLAQQQEPALPAAAPLPQLGSGQMFDRIAHRYDLVNRVLSFR
                     QDIRWRRRVIRELGLDTRKPKKAFAGRGRWFGERPRVLDIATGTGDLAIDIARACEGA
                     TLIGLDPSPGMLEIAQQKIAKRDLSQRISLMVGDAQNLPFANCEMDAATIAFGIRNVP
                     DRAQGLREMARVVRPGGRIAVLELGEPRRGFLGAAARLHSHHLVPRIGGWLSGSREYR
                     YLQQSVAAFPPPDEFANLMRASGLDVIDVIPLTFGVCNLYLATPSEDA"
     gene            8539..9996
                     /locus_tag="VMZ53_27665"
     CDS             8539..9996
                     /locus_tag="VMZ53_27665"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HUS32322.1"
                     /translation="MRVVPAVPASIARPRLVAEYSFVAYPAPVVAAPALVAAFRTGPL
                     VAWSAPASRVTTATATTSLIDAARTPQVIVGMGAAAELRADGSARWRELIAQARTLID
                     NAQLIDLSGAGDHLVRPRLLGGVAFAPGAAGAAPWTDFGDAWFVLPRWTYVHDGTQGA
                     LIFARRGTERVPANELADLRVALAMPFVSRPQPALTAIDHGDRDAWRGQVRAITDAIA
                     RGESTKIVAARSAVVSLAGELRAADMLAELDARHPETVRFLVRPGSGSGDTFVAATPE
                     RLVRLADGIASVDALAGSAARPAAYQTTVQAGSDTPAHSIPACENAKALLASGKDRRE
                     HAIVVDAIVSALQNLGADVRFPDAPGIRKLRHVLHLHTPITAELRTQRHVLELAEALH
                     PTPAVGGTPTRAAIEWIAEREQTPRGWYSSPVGWFDADGNGELAVAIRSGVLTGDRAH
                     LWAGAGIVAGSDPDRELAETDLKLRAMLGALGVSA"
     gene            9993..11885
                     /gene="menD"
                     /locus_tag="VMZ53_27670"
     CDS             9993..11885
                     /gene="menD"
                     /locus_tag="VMZ53_27670"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HUS32323.1"
                     /translation="MSTAAVQTLWAELIAATLADAGVTTCVVSPGSRSTPLVMALAAD
                     GRFELPTIIDERAAAFFALGLARASIPNRAHVGTNTATTERAHVGKSRGVTPVAIVCT
                     SGSAAAHYLPALVEARMSGIPLIAITADRPPELQDCGASQTIDQVGLYGSFVNAAFDL
                     GPPTGSALAMRAVRRKVLQAVTAACGPRPGPVQLEVPLRKPLEPAAPSTDDERALAKV
                     VAELRGKPVMVAPPRLVADASALDALAAAIAAEPKGVIVAGAMPVSFAKARDAVFALA
                     ARAGYPILAEAGSQLRFCSSPAGVTMIDHFDLVLAAKTLAPEPKLVIQIGAEPVGAAW
                     STAFAGAQRWVLADHDWHDADSTAHGVIVGDVASSFATLDASLSSAAPSLAEPHVVAF
                     AAAWAAAEARVVGAVNRALASHPRSEGAVLRAVLEALPATVAIQIGNSLPIRVVDQVC
                     SSTVDRRVLTQRGAAGIDGLIASAAGATRAGAPVLLVLGDVSFAHDVGGLLATREAAA
                     PLAILVLDNGGGRIFDGLPVAKSNVGAAPTASFARHFTTAPQLDPAAVALVLGAQAIT
                     AASPAAAAAAVAAAFAKPGVTVIHAPVTPTGAHDVRRDVLELVPTERGTGRAASGPLA
                     VGARHG"
     gene            11878..12711
                     /gene="menB"
                     /locus_tag="VMZ53_27675"
     CDS             11878..12711
                     /gene="menB"
                     /locus_tag="VMZ53_27675"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HUS32324.1"
                     /translation="MAEAAAPTWNRQPGYEDIIYETSPEGIAKVTINRPEVHNAFRPR
                     TVAEMIRAFDEARDDASIGAVILTGAGTEAFCSGGDQRVRGKGGYVGSDGIPRLNVLD
                     LHMRIRYLPKPVIAMVAGYAIGGGHVLHLVCDLTIAADNAKFGQTGPRVGSFDAGFGS
                     SYLARVVGQKKAREIWFLCRQYNAEQADKMGLVNTVVPIAELEATTIQWCREMLALSP
                     TALRMLKLALNADCDGQAGLMQFAGSATLMYYTTDEGREGKEAFLEKRKPDFSKFPRY
                     P"
     gene            12775..13647
                     /locus_tag="VMZ53_27680"
                     /pseudo
     CDS             12775..13647
                     /locus_tag="VMZ53_27680"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
     gene            13644..15821
                     /locus_tag="VMZ53_27685"
     CDS             13644..15821
                     /locus_tag="VMZ53_27685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUS32325.1"
                     /translation="MRIVAVEHRLVRWTIAPTGPARGLTTRESVIVAVRDDAGHIGLG
                     EAAPLPAAASMSVDTIADALAAVDALASRLPVTIDSPGHASALADRIAAAPAARFAIE
                     CALLAALAQRRGTSVAALLAGTPHAELESAVVVDDEFEAAHASARGARCLKVKVGNNV
                     ADVERVRRIARAAPSCRLRLDANRAWSRGEAIEKLAGFRGLPIEYVEEPCVNSHELLG
                     EPLPCKIALDESLVELAPVDVERAVRSPRLAALVLKPTLLGGFARCMELAALAHRHGV
                     APIVTHALEGPIGFAACGELARAIGADVPVGLAPYAIREVPNASRDARANAGVARGTG
                     AAPARYRVLVATHTDATVDAIREAWQRKETLALLHAKASDVEQARQRRLLDEAARRGD
                     LRDGDAFVLFTSGSTGPSRGVVHTRDSVMAAARASEANLGWRDDDAWLCCLPLAHAGG
                     LSIVMRCLLANKRVILADDPREIADATLASLVPAQLAALLEDPAWRPSRRLRAVLLGG
                     AAAPRPLVDAAVARGVPVLPTYGLTETFGQVATAREPGGVARLLDGVSIDARHDGGLR
                     AVRTAPALLRVRGPMLARAYLDGVPIAPELVTADLGFVQRDSDGDAVHVVGRADDVII
                     SGGENVHPTQVEAVLAATPGVREAAAFGVPDPRWGQIVAVAIAADDRFDREGALARWY
                     AALPAHARPRRIVTLAELPRLPSGKLDRRSVAQLPTIAVEYAG"
     gene            15843..17309
                     /locus_tag="VMZ53_27690"
     CDS             15843..17309
                     /locus_tag="VMZ53_27690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HUS32326.1"
                     /translation="MRTALALVAAATVAACGDDGGNTTRDAAVDAKSFDAPPDADLDI
                     GGSWVDRYISASGTAAVSNCQVAPTGVVIDPATAATTPYAGACKADGTFRIIAPASLG
                     AYFLRLGTSFYETTKRTGLDLSTDHLGRSDIGPIAGVTLNINMLGMDPWAAGDAVYAF
                     SSNIGFSQPITFTTGAPNNGDTALNSVTASWNGYKIDSGKLDTLMLMQIGVHTTAGGL
                     GYTSLDRAFGVPPFTMANNTATAINGVFSAPTAASLQLRIDSASFNQFATVVNPNVTN
                     KTIDGSLFATVSTDAKAPPPLLAFSVPTTGVASLDFGTLSYGDPFATQWQRLMRVQAV
                     FSVPYTYAGISGSRSATMTRVVTRATAAAGIIDAVLGPPTAPTIDNVDAFSATTSTTV
                     PKLTWSPPALGTPTDYEVQAYEVTINGSTLKFTSVLKLTTKQTSVRIPEGLLLGQRQY
                     IFSIASRIRQGVDIYATPLQAGDTSSSAETLTALVTTN"
     gene            complement(17306..18094)
                     /locus_tag="VMZ53_27695"
     CDS             complement(17306..18094)
                     /locus_tag="VMZ53_27695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32327.1"
                     /translation="MRRIAIVTAGLVVLWLVVLVILGAVLGNRYARNTEARVGESLQA
                     SATVASSDLALVRGRLTLDNVAIRRDDAVGHLAIDVVNVRCELAPLGWALIDRDCGEL
                     AVKGVRVEVSTAALFKLKKPKGVKPIVADRVIIEDAQFVFLPSAFAPNLGRIEISIDR
                     AVAGPTRMRTPLSWLFSLEELDARFALPANITVHLQYRMGMMTIAGSIFGSQPITLPV
                     QIPAADAAHDAHEEMQLLLKVGKDVAERVVAQRATDWLRNKLSN"
     gene            complement(18100..20046)
                     /locus_tag="VMZ53_27700"
     CDS             complement(18100..20046)
                     /locus_tag="VMZ53_27700"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HUS32328.1"
                     /translation="MAQAGKYSAEQIQVLEGLEPVRKRPGMYIGGTGKDGYHHLLWEI
                     VDNSIDEAINKHATKVEVTLHKDGKSATVEDNGRGIPVENMAKLKKSALEVIFSTLHS
                     GGKFERGEGYAVSGGLHGVGAAVVNALSKELIATVKRDGERFEMKFSRGEVKQKLKKL
                     GAARGTGTTVYFSPDEEVFGPKLHFDPELIAERLEAKSYLHGGLVLTFTDETKSPATT
                     QEFVHPQGIAEFLPKLVAERGKTPVPPSGTVFYMEKRDDEARLGIELALQWTESTDDL
                     IKSYVNSVPTSDGGTHDTGLRSAVVKAIRNYIATHKLEPKGVTLTAEDIREGLVAVLS
                     SYVHDPQFQSQTKNRLNNPEVAGQVEALVRPALENYLNANPNWGQAVVARVIISARAR
                     EASRAAAQQVSRKTAISHRLNLPGKLADCSSTDPGESELFIVEGDSAGGSAKQGRDRK
                     TQAILPLRGKVLNTEQASGQKLLQNKELQDIVSALGCGIGDTMDLSKLRYGKIFLLMD
                     ADADGHHIATLLMTFFFRHMRPLIDHGHVYLGQPPLYRIDIGKQTFWALDDLHKERLL
                     KANAKANSKPNITRFKGLGEMNPDTLKMTTLDPKSRQALRVTVKDGEQLLTDQVISDL
                     MGKDVAARFKLITENAAEIGDLDV"
     gene            complement(20048..>20810)
                     /locus_tag="VMZ53_27705"
     CDS             complement(20048..>20810)
                     /locus_tag="VMZ53_27705"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HUS32329.1"
                     /translation="VVVTRDGWIKRVRELKDPTQTRTREGDAVTHVLPGSTREKVIFF
                     TNRGSAYVIKINDIAATTGYGDPAQKYFKFGDGEKIVAAMTLDARAMVPPTLLAVSRQ
                     GYGLRFASAQHTEITTKAGRRFAKPKDGDELIGVVPCNDGDIVVAATRDGHVLHCKAD
                     EIAKLEGAGRGVTVIKVADDDIVIGFIAGRKADTFTIETEKSGKKFELKADPKEASSR
                     GGKGHQLMKRVTFRVIEKPVTIQPLANAEGSPGVN"
BASE COUNT         3193 a         7153 c         6824 g         3640 t
ORIGIN      
        1 atcacgatcg gcacttcatg cgagcccagc tcgacgacgc gattgccgta ctcgtcgata
       61 ccctgcagcc gcaagtcgag gcgcttgcct cgctcgagct cgagcttgat cgtgccggtg
      121 ccctcggcct cgaggcgctg ctcggctttg cggtcgacca tgaagaacac gcgcgccccg
      181 acgatcatct tctgcggatc gttcgtcacg acgagcgtca cggcgggcgg ctcatcctgc
      241 gtctcggcct ttgccgcaag cgcgccggct ttgttcgcct tcgcagacgc agcatcgaac
      301 gggcgcgtga ttttcggcga cgtgcctttc ggcagtgacc ccttggggtc gagcgcgagc
      361 cacttcgcga acgcgctctg cgcccctttc tcttcaccga gggcgccctc gacgatcccc
      421 gtcagcttgt agatctcggc aagctcgccg ggcccctgcg tgcccgactt cagcgccgtc
      481 tcgagcagag gcctcgcgct gaggtaatcg gagccgtcga tcgctttacg tgcgtcggcg
      541 agcgcatcgg cgcgcgcatg gtgcacgacg gcgagtagca gcatggtcgc cacggcgacc
      601 tgcctccccc aacgcatgtg caaatcctac cactcgatgc gcttcacagg cgcggtcttt
      661 cccgcctcga tcgtgatgga aagattgcgt gcggagcctg acggtgagac ggcgcgcaca
      721 gcgtgcttgc ccggcgccag cttgatgttg acgagcggcg tttcgccgag cttcttgccg
      781 tccacgtaaa tcacggcgta gacgggcgtc gaatcgatcg tgatgaagcc aggaggaccg
      841 ctcaccgcgg gctcgggctt cttgtcctcg acgggcttct tgtcgtcgag cggcttcttc
      901 tcttccggct tcttggtctc gaccttcttg tcgatcttga ccgtcttctt cttgatcggt
      961 ggcttgggcg gttggtcctc gatgggcttg tcggcgacct tgtcctcggg cttctcgacg
     1021 ggcttctcgt cgacgaccgg aacctcgacc ttctgctcga caaccggcac cgcggcgtcg
     1081 acgaccaccg gcggcggcgt cgcctccggc atcttcacct catcgaccgg ctccttcgcc
     1141 gcgacgacct gcgccttctg cttgtcctgc tggagcttca tgtagagcat cacgcccgtg
     1201 cccgcggccg cagcgacggc gatcagcgcg atgaaccacg gcagccgcga cgatggaatc
     1261 ggcgcagcgg gctgcgacat cgtctgccgc gcctgcggga tcggcggctc tgtctcgtcg
     1321 tagcgccatt cgcggggcgc gaccgaatac ggacccgacg gccgatcttg ctgcgcttcg
     1381 tacggctgcg accgtcgctg cgcggccatc ggcgctgacc ggcgcttctg cgcttccatc
     1441 ggcatcagcg tctcttgatc gtcgaagtct tccgcagagg cgtacgcgtc cgagcgccgg
     1501 ctcggtgcag gcgtgaacgc accgtcggtg ccaaaccgcg cgggcctgac gctcgagtca
     1561 tcgaagtcga gcgaatcgat cgcctgcttc gccgcgacga actgcgcgac ttcctgctgc
     1621 gcctcctgcg cgcgacgatg aatgttcgag accggcgtcg acgagatagc ggagccgtga
     1681 ccgacgagcg ggctcgatgt ctcgagatcg aactggccgc cttcgcgcgc gatccgcaac
     1741 agcatgcgct gctcggcgag ggtcgcctcg aacgaatgcc cgacctcttc gctgatccct
     1801 gcggcattga acggtccacc gagcggcgcg accgcctgcg cgatcgcttc accgagggaa
     1861 cgcgcgctgc cgaatcgatc gtcgggattg cgcgacagag cgcgcgcgat cacgtcgccc
     1921 atgagcggcg gaacatcggg ccgcacttcg cagagatgag gaatcggcag cttcgtgatc
     1981 gcttcgaagg tgaggaactc ggtgtcgcgc ttgaacaggt gcgtctgcgc gagcgtctcc
     2041 cacagcacag cgccgatcgc ccagacgtca gcgcgacgat cgaggccctc gttgcggagc
     2101 tgctcgggcg ccatatacgc gtacgtaccc ttcacggcgc cggtgctcgt gcggatcgat
     2161 tggtcctgga ctttggcgat gccgaagtcg agcaccttca cgatgccgtc gacggtcgcg
     2221 aacagattct gcggcgagat atcgcgatgc acgacgccga gcagctcgcc gtccgcgcgc
     2281 ttcagctgat gcgcgtgatg caggccttcg cacgcttgaa cggcgaaccc cgcgacgagg
     2341 cgaggctgtg gcggtgtcgg atagaagtcc ttgcggcgga acaccgcgag aggaacgccc
     2401 tcgaggtact ccatcgcgat gtagtactgg ccttggaact cgccgagctc gaacacttgg
     2461 cagacgttcg ggtgcgagat gcgcgctgcg acgcgagcct cgtcgaggaa catcgcgacg
     2521 aactgagggt tcttggcgag gtgcgggaga atccgcttga tgcagaccag cttctcgaag
     2581 ccgccgtcac cgtgaaggcg cgcgagaaag atctcggcca tgccaccggt cgcaagcttc
     2641 gcgacgaggg tgtatttgcc gaagcgcctc gcttcggagg cctgcgccgc ctccggtgcc
     2701 atggcggaag tatagctcta taggccaatc caatagttgg ccttgaccat cacgatgttt
     2761 tcgctcgcgg agcgcgcgag agtcgcgaga tcgttgccga gatcgaagag gcccgtgccg
     2821 tcagctgtct ggccgtggct ccagatcgcg aatagagctg aacctgggcg gtactcccac
     2881 ctcagcacca tggtcgagcg caactgaccg aaattgaagt ccggcttgtc gaacgtgaac
     2941 gactcgccgc ccgtgattgg cttgaagcgg tcctcgaagc gatccgcgtg cggattgtcg
     3001 acgtccttgt actcgctgta gctgccgacc gcgacgaacg gctgtgcgta gacctgcagc
     3061 gatagctttg gcgagaacgt ccagttcatg cgcgtcgtga gcgacaccgt cgtcaggcga
     3121 atgcggccga acaggtagtg cgtcatgccc gcggtgtcgt cgatctgatc gacgtactgc
     3181 agcggatcgg tcgcttcggt ccacgtcggg ccgatcgaga cgtcgatgtt cgaacgcgcc
     3241 tggacgaaca agcccgtgtc gacccgcacg agcatgtagt cggtctccgg tttgcgctcc
     3301 gctgcgaaat cgagatcgac gcgaaccttc ttgcgcgagt cgctgttcgc ggtgaggtga
     3361 agcgcgtagc gcggatcggt gcgcagcgcg ggcccaccgc gcatttcgat cggatcgttg
     3421 ccagcgcctt gcacgttgat cgcgccgcgc atggaccagt agttcgcgag ctggccgccg
     3481 atgtccgcgt tgaagccgta gacgacgatc tgcggctcga acgtgttctg catgaacgtg
     3541 atgagactgg tgcgccagtt cagcatcgtc ttgcccgggt cctcgtcgcg atactcggtc
     3601 caggtccacg tctcgttgcg gtccgcatag agctgaaagc ccgcgtcgtt gagctcgagg
     3661 cccggcgtgc ggaagtctcc gccgacgtcc cagcgccaat gcttcgtgtc gccgccgcgg
     3721 ccgaagtacc agcccatgtt cgcgccggcc aggctcgtgc gcttcggatc gaaggcgacg
     3781 tgcgacgcgt cgggccgctg aaacaggtgg cgattcgacg actgcgtgat cgcgatcgcg
     3841 tcctcgctgc cgtgcaccca gctgccgaca ccgtggatgt cgagctccca cgcgttgtcg
     3901 tcccagcggt ggctgagctg cgcgccggcc gtgtacgcct gatcgtgaag cgtcgatacg
     3961 agcggcgtgt cctgcagtgc gcgattcacc gccgtcgccg acacgccgag tgacgtgtgg
     4021 ccttcccgaa ggtctcgctt cacgcgcgcg acggcgtagt tcgtgagcgg cgacacgacc
     4081 gtcgccatct gctgctcgcc gagcgcgatc tgcgcgttct cttcgccggt gaccgcatcg
     4141 aggacgccga cgctccatcc gcgggtcttg cccgtgagct tcgcggcgct gtagatcgtc
     4201 gtcgacgtcg gctgcttgat gtagtcgtag tccatatccg gcgcgtcggg ggcagcgccg
     4261 atgcggcggc tgtagaacac gccctcggcg aagttgtcgc cggtgccgac ttggatcttg
     4321 aagaggtcca caccctcgag gaagaacggg cgcttctccg cgaagaacag ctcgttcgca
     4381 ccgaggttca cctgcgatgg atcggcttcg acttggccga agtcgggatt gagcgtcgcg
     4441 gacagcgtga acgccggacc gaggccgtac ttcacatcga ggccgacgtt gccgagcgcc
     4501 gcggagctcg cgttgagcgg atcgccctgc tcgatcggct cggtctgcag gccgccggtt
     4561 gcgtacggca gcagctcgag gcgtcgcgac ggcttcacct tctcgatgcc gtcgatgacg
     4621 ccgaacttgc tgacgacagc cggcgcggag cgaggccacg gtgaccacgc gctctgctct
     4681 tgattgcgag cgatcgcacg cacgacttgc agcccccatt cctgcgactc ggcgccggag
     4741 aagcggagct gattgagcgg aatgcgaaac tcggcggtcc aaccgttcgt gaccatcgcg
     4801 acgtcgccgg tccacacggc gtcccacgtg tcgtcctgat tcgaatcgtc gaacagcagc
     4861 atgtcgcgct gcacgcctgc cgcgttgagc tgaaacgcga agccggtgcg gcgatcgtga
     4921 tagctgtcga tcccgacgac gacgatatcg gcgagcgcat cgacatcgcg gcgcgtgagc
     4981 gtgcggcgaa tcttgtcggg ctcggtgtct gcgcaccaca cgccgacgta gagcgcgtcg
     5041 tcgtcgtaga gcacggcgaa ccgcgtgtct tgcgtcggct tgtcgccgtc cttcgggaag
     5101 cgctgcgtga agccgccttg cttctcggcg cccgcccacg ctgcgtcgtc cagcttgccg
     5161 tcgatcgcaa caggcgtcgt tcgcctcgtt gcatgcgcac gcaccgacga cggcaacgta
     5221 tccgcagccg cgaacgtgct cgcgagcgcg agggagaggc aaagaagacg agacatgcgc
     5281 gcgtacccaa gagccacaac cagcaccagc ggtggactct tcctggcggc gaatgttgca
     5341 gcatcgccgt tgattcgccc agatgcgaca gcgctgcgac acgtgcggca cgtgatcgga
     5401 gggcttcagc gacgaacttt ggaggtctta tcgggctgct gttcgtcgtg aacgcgtgcg
     5461 tgctcgagca gcagcgcggt gatcgacgtg ttcagctcgt cgcgaccgcc gatcgcgcat
     5521 ggcgagaatt cgaactgacc tgcatgccag tcgagcaacg acatcattcg cgccttcggt
     5581 gcgccattgc cggtgttcgc ttcgaccttg agcactttgc cctccgagac gaacaagcgc
     5641 gcgatctcgc cgtcgcgcag cagcagcaga atgccgctct tgcgctcgaa ctcgaacagc
     5701 gagagcagcg tgccgagccc gatatcgacg aggttgccgc gcaagccggg attggcaacg
     5761 ggcgcggtcg gtgccacgac gcgatgcgtg cggatgacga gctcctcgtc ggtaaacggc
     5821 cgcgggatgt aatcgcgaat gcccgcgcga aatgcttcga gccgaccgag gtcgccgtcc
     5881 tcaccgttca gaatcagcgg gacatcggac agcgtcgcgt gctcgctcat cgcgtacgcg
     5941 aggtcgacgc cctgcatgcc gtcgtcgatc tgcgcagccg agatgatcac gtcgggtcgc
     6001 cactgcgagc atgcatcgag cgcctccggc atcgacgtca cggccgtaac cttgaagccg
     6061 gcaccttcga gacaacgcgt catgcgcgtg cgcagcggcg tccctggctc gaggacgatt
     6121 gcctgcgtgg tcgtcgagag gccggggttc gtgatctcga cgcgaatgct gtcgacgtac
     6181 gcgcgcagct tcagcttcga cggcgcctcg ttgccgatga gctcgaagcc catgcccgga
     6241 tgccggccga gtgccttcgc tgcgtgcggc gtcagcatgt gagcgacgcg cgcttggaac
     6301 gtcagaaacg ttccgtcggg caacgtcagc cggatctcgg tctcttcgcc gacgggaagc
     6361 agcgcgtccg tgcgaatgaa catcccgcgc cccgaaaggt cttcggtctc cgcttcgacg
     6421 gcgccgctgg gcctgtcgaa ctcgactcga cagcggctga ccagtcgctg ctcgcgacct
     6481 cggcgggaat tcgccacaga ccgaaatcgt accggggccg caattccttg tcctctttgg
     6541 gtccctgcgg gatccccgca gatatagaag tcgtcacata accgttactt gccgctcccg
     6601 gttgcatctt caggaagttt caagaatctt tgaaacgtct cggagcatcg gaggagcagc
     6661 ccatgacggt cgcatatgaa gtaacgccag tcggggcatc tgtcctcgac cgactcgcaa
     6721 gcaccagcgg agcgcgtggt gcgcaagacc ttcgtcagcg gttggcgaat ctcgggcgct
     6781 gggtccgcgc ggacctcgtc gagttcgaag ccgagctcgc cacgcttccg aaaggcgcgc
     6841 gcgtcgttca gcaagccgcg catcacctgc tcgatctccg cggcaaacat ctgcgcccgc
     6901 tgtgcgtcgc gctcgcctcc cgcttcggcg agggcttcac gccgcgcgcg cgtggtctcg
     6961 cagtgggtgt cgagctggtc cacaccgcga cgctgctgca cgacgacgtc gtcgatgtcg
     7021 ccgagcgccg ccgaggcaag cccgtggccg cggtcgtgta cggcaacgcc gcgtcgatct
     7081 tcgctggcga ttggctgctc gtcgcggcgc tgcgccgcat cggtgccgcg ggcgtcgacg
     7141 gtctcctcga caagatgctc tcagtgatcg acgagatgat cctcgccgag tcgctgcagc
     7201 tcgaacgccg cggccgcatc gtcgctgatc gcgcggagta cttccgcatc gtcgaaggca
     7261 agacggccgc gctcttccgc tgggcaatgc tcgccggcgc gcgctgcgcg aacctcgacg
     7321 caaagcagga agcggcgctc gagcgctacg gcatccatct cggcgtcgcg ttccaagcgg
     7381 tcgatgacga gctcgacttc gcgggttttg ccgacgcaga gccgagcgaa gagacgggca
     7441 aagatccgct cgtcgatctg cgcgaaggca agatgacgtt ccctctcatc gtcgcgctcg
     7501 aacgcgagcc ggccctgcac gcgcgcatcg aggccgtgct tggcgactcc tctccgaacc
     7561 tcgtcgagct caccgcgatc gccaacgcgg tccgcgccac cggtgctctc gccgccacac
     7621 gcgagctcgc cgagcaacac gtccagctcg cactcgatgc gcttgccgac ctgccggccg
     7681 gaacggcacg cgaatcccta gagaccgtcg cgctcgcttc cctcgagagg cagtcatgac
     7741 gcaactcgca caacagcaag agccggctct ccccgccgcc gcaccgctcc cccagctcgg
     7801 ctccgggcag atgttcgatc gcatcgcgca ccgctacgac ctcgtcaatc gcgtgctctc
     7861 gtttcgccaa gacatccggt ggcggcgacg cgtgatccgc gagctcggcc tcgacacgcg
     7921 caagcccaag aaggcgtttg caggtcgcgg ccgctggttc ggcgagcgtc cgcgcgtgct
     7981 cgacatcgca acgggcacgg gcgatctcgc gatcgacatc gcgcgcgctt gcgaaggcgc
     8041 cacgctgatc ggtctcgatc cgtcgcccgg catgctcgag atcgcgcaac agaagatcgc
     8101 gaagcgcgac ctctcgcagc gcatctcgct gatggtcggc gacgcgcaga acctgccatt
     8161 cgcgaactgc gaaatggacg cggcgacgat cgcgttcggc atccgcaacg ttcccgatcg
     8221 cgcacaaggc ttgcgcgaga tggcgcgcgt cgtccgcccc ggcggtcgca tcgccgtgct
     8281 cgagctcggt gaaccgcgcc gcggattcct cggcgccgcc gcccgcctgc actcgcacca
     8341 tctcgtccca cgcatcggcg gttggctcag cgggtcgcgc gaataccgct acctccagca
     8401 atccgtcgcc gcgttcccac cgcccgacga gttcgccaac ctcatgcgcg catcgggcct
     8461 cgacgtcatc gacgtcatcc cgctgacctt cggcgtctgc aatctctacc tcgcaacgcc
     8521 gtccgaggac gcctgaccat gcgcgtcgtt cccgccgtcc ccgcatcgat cgcgcgcccg
     8581 cggctcgtcg ccgagtactc gttcgtcgcg tacccggcgc ccgtcgtcgc agcgcccgcg
     8641 ctcgtcgcgg ccttccgcac cggtccgctc gtcgcatggt cggcgcccgc atcgcgcgtc
     8701 accacggcaa cggcgacgac ctcactgatc gatgcggcac ggacgccgca ggtcatcgtc
     8761 ggcatggggg ccgcggctga gctgcgcgcc gatggcagcg cgcgctggcg tgagctgatc
     8821 gcacaagccc gcacgcttat cgacaatgct cagctaatcg atctctcggg cgctggcgat
     8881 cacctcgttc gccctcgcct gctcggtggc gtcgcgttcg ctccgggcgc tgccggcgct
     8941 gcgccatgga ccgatttcgg cgacgcgtgg ttcgtgttgc cgcgctggac gtatgtccac
     9001 gacggtaccc agggcgcact gatcttcgcg cggcgcggca ccgagcgcgt gccggcgaac
     9061 gagcttgctg acctgcgcgt cgcgctcgcg atgccgttcg tttctcgccc gcagcccgcg
     9121 ctcactgcga tcgatcacgg agatcgagac gcgtggcgcg gtcaggtacg tgccatcacc
     9181 gacgcgatcg cccgaggcga gtccaccaag atcgtcgctg cgcgcagcgc ggtggtctcg
     9241 ctcgccggcg agctgcgcgc cgccgacatg ctggcggagc tcgacgcgcg tcatccggag
     9301 acagtgcgct tcctcgtgcg gcccggctcc ggatcaggcg acacgttcgt cgcagccacg
     9361 cccgagcgtc tcgttcggct cgccgatggg attgcgtccg tcgatgcgct cgctggctcc
     9421 gccgcgcgtc ccgcggccta tcaaacgacc gtccaagcgg gcagcgacac gccggcccac
     9481 agcatccctg cgtgtgagaa cgccaaggcg ctgctcgcga gcggcaaaga ccgccgcgag
     9541 cacgcgatcg tcgtcgacgc gatcgtttcc gcgctacaaa atctcggcgc cgatgtgagg
     9601 ttcccggacg cgccgggaat ccgcaagctc cgccacgtgc tgcatctgca cacgcccatc
     9661 accgccgagc tgcgcacgca gcgccacgtg ctcgagctcg ccgaagcgct gcacccgacg
     9721 cccgccgtcg gtggcacgcc cacgcgcgcc gccatcgaat ggatcgcgga gcgcgagcaa
     9781 acgccgcgcg gctggtactc ctctcccgtc ggctggttcg acgccgacgg caacggcgag
     9841 ctagccgtcg cgatccgctc cggcgtcctc accggtgatc gcgctcacct ctgggcgggc
     9901 gccggcatcg tggcgggcag cgaccccgat cgcgagctcg ccgaaaccga cctcaagctg
     9961 cgcgccatgc tcggtgcgct cggagtcagc gcatgagcac cgcagcggtg cagacgctgt
    10021 gggccgagct gatcgcggca acgctcgccg acgcgggcgt gacgacgtgc gtggtcagcc
    10081 ctggctcgcg ctcgacgccg ctcgtgatgg cgctcgccgc cgacggtcgg ttcgagctgc
    10141 cgacgattat cgacgagcgc gccgccgcgt tcttcgcact cggcctcgcc cgcgcatcga
    10201 tccctaaccg tgcccacgtg ggcacgaaca ccgcgacgac cgaacgtgcc cacgtgggca
    10261 agtcgcgcgg cgtgacgccc gttgcgatcg tctgcacgag cggcagcgcc gcggcgcact
    10321 acttgcccgc cctcgtcgaa gcgcgcatgt cgggcattcc gctcatcgcg atcaccgcgg
    10381 atcggccgcc ggagctgcag gattgcggcg cgagccagac catcgatcag gtcgggctgt
    10441 atggctcgtt cgtgaatgcg gcattcgatt tggggccgcc cactggcagc gcgctcgcga
    10501 tgcgcgcggt gcgccgcaaa gttctgcagg ccgtgaccgc ggcatgcggg ccgcgaccgg
    10561 gccccgtcca gctcgaagtt ccgctgcgca agccactcga gccggcagcg ccgtccaccg
    10621 acgacgaacg cgcgctcgcg aaggtcgttg cggagctgcg cggcaagccg gtgatggtcg
    10681 cgccaccgcg cctcgtcgcc gatgcgagcg cgctcgacgc gctggccgcg gcgatcgcgg
    10741 ccgagccgaa aggcgtcatc gtcgccggcg cgatgccggt ctcgtttgcg aaggcgcgcg
    10801 atgcggtctt cgcgctcgcg gcgcgcgcgg ggtatcccat tctcgcggaa gcgggcagcc
    10861 agctgcggtt ctgcagcagc cccgctggcg tgacaatgat cgaccacttc gatctcgtgc
    10921 tcgcggcgaa gacgttggcg ccggagccga agctcgtcat tcagatcggc gcggagcccg
    10981 tcggcgcggc gtggtcgacg gcgtttgccg gtgcgcagcg ctgggtcctc gccgatcacg
    11041 actggcacga cgccgactcg accgcgcacg gcgtgatcgt cggcgatgtg gcgagctcgt
    11101 tcgcgacact cgacgcctcg ctcagcagcg cggctccctc gctcgcagag ccgcacgtcg
    11161 tagcatttgc ggccgcgtgg gcggcggcgg aagcgcgcgt tgtcggcgcc gtgaatcgcg
    11221 cgctcgcttc gcatccccgc agcgaaggtg cggtgctgcg cgcggtgctc gaggcgttgc
    11281 ccgcgaccgt cgcgattcag atcggcaaca gcttgccgat tcgcgttgtc gatcaagtgt
    11341 gctcgtcgac ggtggaccgg cgcgtgctca cgcagcgcgg cgctgcgggc atcgatggac
    11401 tcatcgcctc ggcggctgga gcgacgcgag ctggcgcgcc tgtcttgctt gtcctcggcg
    11461 acgtgagctt cgcgcacgac gtgggcggct tgctcgcgac gcgcgaggca gcggcaccgc
    11521 tcgcgattct tgtcctcgat aatggcggcg gccggatctt cgacgggctg cccgtcgcga
    11581 agtcgaatgt cggggcggcg ccaacggcct cgttcgcccg gcacttcacg accgcgccgc
    11641 agctggatcc ggcggcggtc gcgctcgtgc tcggcgcgca ggcgatcacg gcggcatcac
    11701 ccgcggcagc ggcagcggca gtcgccgcgg catttgccaa gcccggagtc accgtcattc
    11761 atgcgcctgt cacgccgacc ggcgcgcacg atgttcgtcg cgacgttctc gagctcgttc
    11821 ctacggaacg cggcacggga cgcgcggcgt ctggaccact tgcagtagga gctcgtcatg
    11881 gctgaagcag cagcaccgac ctggaaccgt caacccggct acgaagacat catttacgaa
    11941 acgtcgcctg agggaatcgc caaggtcacg atcaatcgcc cggaggttca caacgcgttt
    12001 cgcccgcgca ccgtcgcgga gatgattcgc gcgttcgacg aggcgcgcga cgacgcatcg
    12061 atcggcgcgg tgatcctgac gggcgcgggc accgaggcgt tctgctccgg cggcgatcag
    12121 cgcgtgcgcg gcaagggcgg ctacgtcggc tccgacggca ttccgcgcct caacgtcctc
    12181 gatctgcaca tgcgtattcg ctacctgccg aaaccggtga tcgcgatggt cgcgggctac
    12241 gcgatcggtg gcggccacgt gctgcacctc gtctgcgatc tgacgatcgc ggcggacaac
    12301 gcgaagttcg gccagaccgg tcctcgcgtc ggtagcttcg atgcgggctt tggtagctcg
    12361 tatctcgctc gcgtcgtcgg tcagaagaag gcgcgcgaga tctggtttct ctgccgccag
    12421 tacaacgcgg agcaggccga caagatgggc ctcgtcaaca cggtcgtccc gatcgccgag
    12481 ctcgaagcca ccacgatcca gtggtgtcgc gaaatgctcg cgctatcgcc gacggcactg
    12541 cgcatgctga agctagcgct gaacgcggac tgcgacggcc aagctggcct catgcagttc
    12601 gccggctcgg cgacgctcat gtactacacg accgacgaag gtcgcgaagg caaagaagcg
    12661 ttcctcgaga agcgcaagcc cgacttctcg aagttcccgc gctacccgtg agccaaccat
    12721 gacgatctcg atcgcagcag ccgctcgtcg ccacacgcaa cccgcgaggt tggcgtcgtg
    12781 agcgcgtgga tcgcagccgc tcgactccgc acgctccccg cagcggtcgt acccgttgtc
    12841 gtcgggacgg ccgttgcgca tgcggtgcgc gcgatcgcgt ggggccctgc cctcgccgcg
    12901 ctcggcggcg cgctcgcgat ccaggtcgcc acgaacttcg cgaacgatgt gttcgacgcc
    12961 gagtccggcg ctgacggacc ggaccgcatc ggtccaacac gcgcggtcgc ggcaggcctc
    13021 atcagtgccg cggcgatgaa gcgcgcgatg atcgcagcgt tcgcggtcgc gagcctgttc
    13081 ggcctctacc tcgtgtcgac ggcgggctgg cccatcgtcg ccatcggtct cgcatccatc
    13141 gcggcgggca tcgcgtacac cggcggaccg tggccgctcg gctatcacgg cctcggcgat
    13201 gtgttcgtgc tcgcgttctt tggcttcgtc gcggtgtgcg gcacggccta cgtgcagctc
    13261 ggctacgtgc ccggcctcgc ggtcgcggcg tcgattcccg tcggcgcgct cgcaaccgcg
    13321 atcctcgtcg tcaacaacat ccgcgatcgc tcgaccgacg aacgcgcagg caagcgcacg
    13381 ctcgcggtgc ggttcggccg ccgcgctgca atcgtcgagt acgccatgct gctggtcgct
    13441 gcgtacgcaa cgcctctcgc gctcgctatc tgtcatcgct cggcgtggct cgcgctgccc
    13501 gtcctcaccg cgccgatcgc ctggctgcgc ctgcgccagc tcatcggcgc ggcaagcggc
    13561 ccggtcttca acggtctgct cgccgcgacc gcgcagctga tgctgctcca cggcgtgctg
    13621 ttcgcgacgg ggcttgtcgt gtcatgagga tcgtcgcggt cgaacatcgc ctcgtgcgtt
    13681 ggacgatcgc gcccaccggt cccgcgcgag gtctcacgac gcgcgagtcg gtcatcgtcg
    13741 cagtgcgaga cgacgccggc cacatcggcc tcggcgaggc tgcgccgctc cctgccgcgg
    13801 cgtcgatgtc cgtcgatacg atcgccgatg cgctcgccgc cgtcgacgcg ctcgcctcgc
    13861 ggctccccgt cacgatcgat tcaccggggc acgcgagcgc actcgccgat cgcatcgccg
    13921 ccgcccccgc tgcacgcttc gcgatcgagt gcgcacttct cgcggcgctc gcgcagcgtc
    13981 gcggaaccag cgtcgccgcg ctgctcgccg ggacgcctca cgccgagctc gaatcggccg
    14041 tcgttgtcga cgacgaattc gaagcggcgc acgcctccgc tcgaggagct cgctgcctca
    14101 aggtcaaagt cggcaacaac gttgcggacg tcgagcgcgt cagacggatc gcgcgcgccg
    14161 cgccttcgtg caggttgagg ctcgatgcga atcgagcgtg gtcacgcggc gaggcgatcg
    14221 agaagctcgc gggatttcgt gggttgccga tcgagtacgt cgaggagcca tgcgtgaatt
    14281 cgcatgagct gttgggcgag ccgttgccgt gcaagattgc gctcgacgag agccttgtcg
    14341 agctcgcgcc cgtcgacgtc gaacgtgcgg tgcggtcgcc gaggcttgcg gcactcgtgc
    14401 tgaagccgac gctgctcggc gggtttgctc gctgcatgga gctcgctgcg ctcgcgcatc
    14461 ggcacggtgt cgcgcccatt gtcacgcacg cgctcgaggg gccgatcgga tttgcggcgt
    14521 gcggcgagct cgcgcgggcg attggcgcgg acgtcccggt tgggctcgca ccgtacgcca
    14581 ttcgcgaggt gccgaacgcg agtcgcgacg cgcgagcgaa cgcgggcgtt gcccgcggca
    14641 ccggcgcggc gccggcacga tatcgcgtgc tcgtcgcgac gcacaccgat gcgacggtcg
    14701 atgcgattcg cgaggcgtgg cagcgcaagg agacgctcgc gctcctacac gcgaaggcaa
    14761 gcgacgtcga gcaagcgcga cagcgccggc tcctcgacga ggccgcgcgg cgtggcgacc
    14821 tgcgcgacgg cgacgcgttc gttctcttca cgtccgggtc caccggcccg tcgcgcggcg
    14881 tcgtgcatac gcgcgacagc gtgatggcag cggcacgcgc gagcgaggcg aacctcggct
    14941 ggcgcgacga cgatgcgtgg ctgtgttgct tgccgctcgc tcatgccggc ggtttgtcga
    15001 tcgtcatgcg ctgcttgctc gcgaacaagc gcgtcattct cgccgacgat ccgcgcgaga
    15061 ttgccgatgc gacactcgcg tcgctcgttc ccgcgcagct cgccgcgttg ctcgaggatc
    15121 ctgcatggag gccgtcacgg cgcctgcgcg ccgtcttgct cggcggtgca gctgcgccgc
    15181 gtccgctcgt cgacgcagca gttgctcgtg gcgtgccggt gttgccgacg tacggcctga
    15241 cggagacgtt cggtcaagtc gcgaccgcgc gcgagccagg cggtgtcgcg agactgctcg
    15301 acggcgtctc gatcgatgcg cgacacgatg gcggcctgcg cgcggtccgc actgcacctg
    15361 cgctgcttcg cgtgcgtgga ccgatgctcg cgcgtgcgta cctcgacggc gtgccgatcg
    15421 caccggagct cgtcaccgcg gacctcggct tcgtgcagcg ggacagcgac ggcgacgccg
    15481 tccatgtcgt cggtcgcgcg gacgacgtga tcatcagcgg cggcgagaac gtgcatccca
    15541 cgcaggtcga ggccgtgttg gctgcgaccc cgggcgtgcg cgaggcggcg gcgttcggcg
    15601 tgccggatcc gcggtgggga cagatcgtcg cggtcgcgat cgcggcggac gaccggtttg
    15661 atcgcgaagg tgctctcgcg cgttggtatg ccgcgctgcc tgcacacgcg cggccacgac
    15721 gcatcgtcac gctggcggaa ttgccgcggt taccgtcggg aaagctcgac cgccggagcg
    15781 tcgctcagct gcccacgatt gcggtcgaat acgctggtta gacgtgatag gcctcccgct
    15841 tcatgcggac ggcattggct cttgtggcgg cggcgacggt ggcagcgtgt ggcgacgatg
    15901 gcggcaacac gacgcgcgac gcagcggtag acgcgaagag cttcgatgcg ccgcccgatg
    15961 cggacctcga catcggtggc agctgggtcg atcgctacat ctccgcgagc ggcacggccg
    16021 ccgtttccaa ctgccaggtc gcgccgacgg gcgtcgtcat cgatccggca acggccgcga
    16081 caactcccta tgccggcgcg tgcaaggcag acgggacgtt tcgcatcatc gccccggcga
    16141 gcctcggcgc gtattttctt cgcctcggca catcgttcta cgagacgact aagcgcaccg
    16201 gcctcgacct ctcgacggat cacctcggac gctccgacat cgggccgatc gccggcgtaa
    16261 cgctgaacat caacatgctc ggcatggacc cgtgggccgc cggcgacgcg gtttacgcgt
    16321 tctcgtcgaa catcgggttc tcgcagccga tcacattcac gacgggtgca ccgaacaacg
    16381 gcgacaccgc gctcaattcc gtcaccgcat cgtggaacgg ctacaagatc gacagcggca
    16441 agctcgacac gctcatgctg atgcagatcg gcgtgcacac gaccgcaggc ggccttggct
    16501 acacgtcgct cgaccgggcg ttcggcgtcc cgccattcac gatggcgaac aacacggcga
    16561 ccgcgatcaa cggcgtgttc agcgctccga cggctgcatc actgcagctg cgcatcgaca
    16621 gcgcgagctt caatcaattt gcgaccgtcg tgaatccgaa cgttacaaac aagacgatcg
    16681 atggctcgct gttcgcgacg gtgtcgacgg acgcgaaggc gccgccacct cttctcgcat
    16741 tcagcgtgcc gacgaccggc gtggcatcgc tcgacttcgg cacgctgtcg tacggcgatc
    16801 cgttcgcgac gcagtggcag cgcctcatgc gcgtccaagc ggtgttctcg gttccataca
    16861 cgtacgccgg catctcgggc tcgcgcagcg cgacgatgac tcgcgtggtg acgcgcgcga
    16921 ccgcggccgc cggaatcatc gacgcggtcc tcggcccacc gacggcgccg accatcgaca
    16981 atgtcgacgc gttcagtgcc accacgtcga cgacggtgcc gaagctgacc tggtctccac
    17041 cggcgctcgg cacgccgacg gactacgagg tccaggccta cgaagtcacg atcaacggca
    17101 gcacgctcaa gttcacctcg gtcctcaagc tcacgacgaa gcagacgtcc gtgcggattc
    17161 ccgagggact tctcctcggt cagcgccagt acatcttctc gatcgcgtcg cgcattcgcc
    17221 agggcgtcga catctacgcg acaccgctgc aagccggtga cacgtcgtcg agcgcagaga
    17281 cgctcaccgc tctcgtgacg accaactagt tcgacagctt gttgcgaagc cagtcggtcg
    17341 cgcgctgagc gacgacgcgc tcggcaacgt ccttgccgac cttcaacagc agctgcatct
    17401 cctcgtgcgc atcgtgtgcg gcatcggccg cggggatttg cacgggcaac gtgatcggct
    17461 gcgagccgaa gatcgatccg gcgatcgtca tcatccccat tcgatactgc agatgcacgg
    17521 tgatgttggc gggcaacgcg aagcgcgcgt cgagctcctc gagcgagaac agccacgaca
    17581 gcggcgtgcg catgcgcgtc ggccctgcca ccgcgcgatc gatcgagatc tcgatgcggc
    17641 ccagattcgg cgcgaacgcg ctcggcaaga acacgaactg cgcatcctcg atgatcacgc
    17701 ggtcggccac gatcggcttc acgcccttcg gcttcttgag cttgaagagc gcggccgtcg
    17761 agacttcgac ccgcacgcct ttgaccgcga gctcgccgca atcgcgatcg atcagcgccc
    17821 agccgagcgg cgccaattcg cagcggacat tgacgacatc gatcgcgaga tgcccgacgg
    17881 cgtcatcgcg gcgaatcgcg acgttatcga gcgtcagccg cccgcgaacg agcgcgaggt
    17941 cactgctcgc gaccgtcgcc gaggcctgca gcgattcgcc gacgcgcgcc tcggtgttcc
    18001 gcgcgtagcg attgccgagc accgcgccga ggatcaccaa caccacgagc cagagcacga
    18061 cgagcccggc tgtgacgatg gcgatgcgcc gcaacaacct caaacgtcga ggtcgccgat
    18121 ctctgctgcg ttctcggtaa tgagcttgaa gcgcgccgcc acgtccttgc ccatgagatc
    18181 ggagatgacc tgatcggtga gcagctgctc gccgtccttc accgtcacgc gaagcgcttg
    18241 gcgcgacttc ggatccagcg tggtcatctt cagcgtgtcc gggttcatct cgccgaggcc
    18301 tttgaaccgc gtgatgttcg gcttgctgtt ggccttggcg ttcgccttga gcagccgctc
    18361 tttgtgcaga tcatcgagag cccagaacgt ttgcttgccg atgtcgatgc gatagagcgg
    18421 cggctggccg agatacacgt gcccgtgatc gatcagcggg cgcatgtggc ggaagaagaa
    18481 cgtcatcagc agcgtcgcga tgtgatggcc gtcggcatcg gcgtccatca gcaagaagat
    18541 cttgccgtag cgcagcttgc tgaggtccat cgtgtcgccg atgccgcagc cgagtgcgct
    18601 gacgatgtcc tgcagctctt tgttctgcag cagcttctgg ccactcgcct gctcggtgtt
    18661 cagcaccttg ccgcgcagcg ggagaatcgc ctgcgtcttg cgatcgcggc cctgcttggc
    18721 cgagccacct gcggaatcgc cctcgacgat gaacagctcg ctctcgcccg gatccgtcga
    18781 cgaacagtcg gccagcttgc ccgggaggtt gagacggtgg ctgatcgccg tcttgcgcga
    18841 gacctgctgc gccgccgcac gcgatgcttc acgcgcacgc gccgagatga tcacgcgcgc
    18901 gacgaccgcc tggccccagt tcgggttcgc gttgagatag ttctcgagcg caggacgcac
    18961 gagcgcttcg acttggcccg cgacctcggg attgttgagg cggttcttcg tctgcgactg
    19021 gaactgcgga tcgtgcacgt acgacgagag caccgcgacg agaccttcgc ggatgtcctc
    19081 ggccgtgagc gtcacgccct tgggctcgag cttgtgcgtc gcgatgtagt tgcggatcgc
    19141 tttcacgacg gcagagcgca agcccgtgtc gtgcgtgcca ccgtcggagg tcggcaccga
    19201 gttgacgtac gacttgatca agtcgtcggt cgactccgtc cactgcagag cgagctcgat
    19261 gccgaggcgt gcctcgtcat ctctcttctc catgtagaag acggtgccgc tcggcggaac
    19321 aggcgtcttg ccgcgctcgg cgacgagctt cgggaggaac tccgcgatgc cctgcgggtg
    19381 gacgaactcc tgcgtcgtgg ccggcgactt cgtctcgtcg gtgaacgtca gcacgaggcc
    19441 accgtgcagg tagctcttcg cctcgaggcg ctccgcgatc agctctggat cgaagtgcaa
    19501 cttcgggccg aacacttctt cgtcgggcga gaagtagacc gttgtgccgg taccgcgcgc
    19561 agcgccgagc ttcttgagct tctgcttcac ctcgccacgc gagaacttca tctcgaagcg
    19621 ctcgccgtcg cgcttcacgg tcgcgatgag ctccttcgac agtgcgttca cgactgccgc
    19681 gccgacgccg tgcagaccgc cggacaccgc gtagccctca ccgcgctcga acttgccgcc
    19741 cgagtgcagc gtcgagaaga tcacctcgag cgcgctcttc ttgagcttgg ccatgttctc
    19801 gacggggatg ccgcggccgt tgtcctcgac cgtcgcgctc ttgccgtcct tgtgcagcgt
    19861 gacctcgact ttggtcgcgt gcttgttgat cgcttcgtcg atcgagttgt cgacgatttc
    19921 ccagagcagg tggtgatagc cgtccttgcc ggtaccaccg atgtacatgc ccgggcgttt
    19981 gcgaaccggc tcgagcccct cgagtacctg gatctgctcc gcggagtatt tacctgcctg
    20041 cgccatttca gttcactccg gggctgccct cggcgttcgc caggggctgg atcgtgacgg
    20101 gtttctcgat cacgcggaac gtcacgcgct tcatcagctg gtgacccttg ccgccgcgac
    20161 tcgaggcttc cttcgggtcg gccttcagct cgaacttctt gccgctcttc tcggtctcga
    20221 tcgtgaaggt gtcggccttt cgacccgcga tgaagccgat cacgatgtcg tcgtcggcga
    20281 ccttgatcac cgtaacaccg cgaccagcgc cctcgagctt cgcgatctcg tccgccttgc
    20341 agtggagcac gtgaccatcg cgcgtcgccg caacgacgat gtcgccatcg ttgcacggca
    20401 ccacgccgat cagctcgtcg ccgtctttgg gcttcgcgaa ccggcggcct gccttcgtcg
    20461 tgatctcggt gtgctgcgcc gacgcgaacc ggaggccata gccctgccgc gacacggcga
    20521 gcagcgtcgg cggaaccatc gcgcgcgcat cgagcgtcat cgccgcgacg atcttctcgc
    20581 cgtcgccgaa cttgaagtac ttctgcgccg gatcgccgta gcccgtcgtc gctgcgatgt
    20641 cgttgatctt gatgacgtac gcgctgccac ggttcgtgaa gaagatgacc ttctcgcggg
    20701 tcgagcccgg cagcacgtgc gtgaccgcat cgccttcgcg cgtacgcgtc tgtgtcggat
    20761 ccttcagctc gcgaacgcgc ttgatccagc catcgcgcgt gacgacgacg
//
LOCUS       DATLBZ010000551         2513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1468364, whole genome shotgun sequence.
ACCESSION   DATLBZ010000551 DATLBZ010000000
VERSION     DATLBZ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468364"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VMZ53_27710"
     CDS             <1..209
                     /locus_tag="VMZ53_27710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32330.1"
                     /translation="DLPADVDEILSRLLVKDREQRYPSAAALAGTIGILLSSLPPSEG
                     ATLIEPSLPIEEDVTSLGPPPDKE"
     gene            complement(210..>2513)
                     /locus_tag="VMZ53_27715"
     CDS             complement(210..>2513)
                     /locus_tag="VMZ53_27715"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HUS32331.1"
                     /translation="WNAETGADFTTYQTVGTSEHLSELLALEVNRIAIGCNGLTPEIF
                     AREREVVVNEMRTRQGATGASIQRVIAEAIFPAGHPYRRVDSADTVAKLELKDACDFI
                     AANYQRGKAVVVVSGDVDEGRLQKAAASQFGRLKKRIPAIRGAVAAVTPTSGKVRLKA
                     DVDEPMLIATWPLPAMDTKDYRMVEMAAEWIDSELEQFAFIYEWGHSSFTTVLGGPRA
                     PILAVGIELNSTGDADEAIDSVEKSVRFSLEMLGSEKESERWQSHWRGRAETLLATWE
                     SLSARNALFADILSYDHDDGYLIGRVAEVQKSSPGSVRSVAKPWLSTAKYLVIEPTGT
                     VQSTSASTFAGGAEEIAVAVDGTLADNPLPKPPVRPLLSADRYTLDNGLEVILWPYGS
                     TPLVRARLVVDSGSAHDPAGKEGIAELVGAGEVYPDSLVFSTRDLSIFVDDVVARTVA
                     ELRLPGYGLSDEAKKYIKARLSAPRAEERREYERKMLVEVYGDGHPYARSPMSPKSID
                     KLHHDLVNDWARSHLVPKNSTLIVAGQFDPELVKKHIAYDVGHVSGGKDSDDIFVAHT
                     KSIAEGFVMGEATKPSPTVELDVMWQTVNGLDKDHAKRLVLEQLLDDELAVLRSKHAI
                     TYGFYASYEPRKAGGLWRVSGEADASRADAATKILLDVLDQMRSDPEAIRAAFVRARE
                     KTLQALLATTADTAVVVNRLATIARFDKPDDFYHRLPEKVAELTLADFHHFLVSELPS
                     DGQVFGAFGQGNAVARAVGTARQHTPK"
BASE COUNT          421 a          820 c          785 g          487 t
ORIGIN      
        1 ccgacctccc cgcggacgtc gacgagatcc tctcgcgcct gctcgtcaaa gaccgcgagc
       61 agcgttaccc gtcggccgct gcgctcgcgg gaacgatcgg cattttgctc tcctcgctgc
      121 cgccgtcgga aggcgcgacg ctcatcgagc cctcgctgcc gatcgaggaa gatgtgacct
      181 cgctcggccc accgcccgac aaagagtagt cacttcggcg tgtgctgccg ggccgtgccg
      241 accgcgcgtg cgacggcgtt gccttgcccg aatgcaccaa acacttggcc gtcggacggc
      301 agctcgctga cgaggaaatg gtgaaagtcg gcaagcgtga gctccgcaac tttctcgggc
      361 agacggtgat agaagtcatc cggcttatcg aatcgcgcga tcgtcgcgag gcgattcacg
      421 acgacagcgg tgtcggccgt cgtcgcgagc aaggcctgca gcgttttctc tcgcgcacga
      481 acgaatgccg cccgaatcgc ctccggatcg ctgcgcattt ggtcgagcac gtcgagcaag
      541 atcttcgtcg ccgcatcggc gcgacttgca tccgcttcgc cgctgacccg ccagaggcca
      601 cccgctttgc gcggctcgta cgacgcatag aagccgtacg tgatcgcatg cttcgagcga
      661 agcacggcga gctcgtcgtc tagcagctgc tcgaggacga gtcgcttcgc atgatctttg
      721 tcgaggccat tgacggtctg ccacatgacg tcgagctcga cggtaggtga tggcttcgtc
      781 gcctctccca tgacgaatcc ctcggcaatg ctcttcgtat gcgcgacgaa aatatcgtcg
      841 gagtctttgc cgccgctgac gtgtccgacg tcgtacgcga tgtgcttttt cacgagctcg
      901 ggatcgaatt gaccggcgac gatcaaggtc gagttcttcg gcaccagatg tgatcgagcc
      961 cagtcattga cgagatcgtg atggagcttg tcgatgctct tcggcgacat cggcgatcga
     1021 gcgtacgggt gaccatcgcc gtacacctcg acgagcatct tgcgctcgta ctcgcggcgt
     1081 tcctcagcgc gcggcgcgga cagccgggcc ttgatgtact tcttcgcttc atcgctgagg
     1141 ccgtagcccg gtagtcgcag ctcggccacg gtgcgcgcga caacgtcgtc gacgaagatc
     1201 gagagatctc gcgtgctgaa gacgaggctg tcgggataga cctcgcctgc accgacgagc
     1261 tctgcgatgc cctctttgcc ggctggatcg tgtgcggagc ccgagtcgac gacgagccgc
     1321 gcgcggacga ggggcgtcga gccatacggc cagaggatga cctcgaggcc gttgtctagc
     1381 gtgtagcggt cagcgctcag cagcggccgc accggcggtt tgggcagcgg gttatcggcg
     1441 agcgtgccgt caacggcgac tgcgatttcc tcggccccgc cggcgaacgt cgacgcgctc
     1501 gtcgactgca cggtgccggt cggttcgatc acgagatact tcgcggtgct cagccacggt
     1561 ttcgcgacac tgcgcaccga gcctggactc gacttttgca cttcagcaac gcgaccgatc
     1621 aggtagccgt cgtcgtggtc gtacgaaagg atgtctgcaa agagcgcatt gcgcgcggag
     1681 agcgattccc acgtcgccag cagcgtctcg gcgcggcctc gccagtgaga ctgccagcgc
     1741 tcgctctcct tctcgcttcc gagcatctct agtgagaagc gaacggattt ctcgacgctg
     1801 tcgatcgctt cgtccgcatc accggtcgag ttgagctcga tacccacggc gaggatcggc
     1861 gcgcgcggac cgccgagcac tgtcgtaaac gacgagtggc cccactcgta gatgaacgcg
     1921 aactgttcga gctcgctgtc gatccattct gccgccatct cgaccattcg atagtctttg
     1981 gtgtccattg ccggcagcgg ccacgtcgcg atcagcatgg gctcgtcgac gtctgctttg
     2041 agccgaacct ttcctgacgt gggcgtcacg gctgccacag cgccgcggat ggccgggatg
     2101 cgcttcttca agcggccgaa ttgcgatgca gccgctttct gcagccgacc ctcgtcgaca
     2161 tcgccgctga cgacgacaac ggctttcccc cgctgatagt tggccgcgat gaagtcgcac
     2221 gcgtctttga gctcgagctt ggcaaccgtg tccgctgaat cgacgcgccg atagggatgc
     2281 ccggcgggaa agatcgcctc cgcgatcact cgctggatcg acgcgccggt tgcgccctgc
     2341 cgcgtacgca tctcgttgac cacaacctcg cgctcgcgcg caaagatctc gggcgtcagc
     2401 ccgttgcagc cgatcgcgat tcgattgacc tcgagcgcga gcagctcgct cagatgttcg
     2461 gaggtgccga cggtctgata cgtcgtgaag tccgcgcctg tctccgcgtt cca
//
LOCUS       DATLBZ010000552         3688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7073558, whole genome shotgun sequence.
ACCESSION   DATLBZ010000552 DATLBZ010000000
VERSION     DATLBZ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7073558"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VMZ53_27720"
     CDS             <1..447
                     /locus_tag="VMZ53_27720"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HUS32332.1"
                     /translation="YVLLPDAVDGLPAIEAKLADTIRALQGKLAEARVSLALPKFTID
                     PPGPLELQQTLGELGIKTAFTPAADFSGITKTTTEPLFISNVMHKAFVKVDEKGTEAA
                     AATAIGAPYGAPPAPGTPFVADHPFVFMIIDRSSGLILFIGRVAAP"
     gene            complement(448..795)
                     /locus_tag="VMZ53_27725"
     CDS             complement(448..795)
                     /locus_tag="VMZ53_27725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32333.1"
                     /translation="MLAALIFWPLLATASARVDVTGDYASNWDDVHLVQEGDRIHGTY
                     VCCGGGTIEGRIIEGRIIKFEWDEPRGAGHGAGIWTIKGSTLQGTWGHAQSNSDGGPW
                     TLERKTKRSQIAN"
     gene            complement(835..1554)
                     /locus_tag="VMZ53_27730"
     CDS             complement(835..1554)
                     /locus_tag="VMZ53_27730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32334.1"
                     /translation="MRSAWLLAIAVVTACAAEAPDADHDEVEDFIVDSADGKADGVPA
                     TFDANNVVDDALLTNGGGMSVADVQAFFEKNPYGTRSWLASYKVNGVSAAQMVVEAAQ
                     THDVNPLILIARMQVETSLVSKTATPSERMINRALGCGCPDGASCNTAYSGLQRQLDC
                     GARTLRRWYDASIDGSGQWRKGVSRSTLDPRRVTPGNHATATFYAYTPWVLVGSGGTW
                     LAWNVTRKYVRYAEGANLLHN"
     gene            complement(1676..2290)
                     /locus_tag="VMZ53_27735"
     CDS             complement(1676..2290)
                     /locus_tag="VMZ53_27735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32335.1"
                     /translation="MRRFVLVAALLIACSHNKPAAPPSAPPPPQASPLSDRMDTFEMN
                     GYTLAGAEYWPYVGKDDIRYPEEVLWGFYPEKGVIPPGETEPNVDSANPDAVDCAQRS
                     FTALQVFLAKNPPELRQIVERGAASGYVPRFYLWTNDYSKAAEPYPPGVREARLWYWK
                     RKQPEPPKPPGYWKWEATLTQKGECQIPSDEQIKTYLAETLAKL"
     gene            complement(2310..2564)
                     /locus_tag="VMZ53_27740"
     CDS             complement(2310..2564)
                     /locus_tag="VMZ53_27740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32336.1"
                     /translation="MNCRDGCGACCIAPSISPCVALPEGKAAGVRCPHLSSELRCVLF
                     GKPERPATCVGLRPQLEMCGASAEEAMATLVSWESLTAPR"
     gene            complement(2605..2919)
                     /locus_tag="VMZ53_27745"
     CDS             complement(2605..2919)
                     /locus_tag="VMZ53_27745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HUS32337.1"
                     /translation="MSKKKPARPERTAKREKEREVRQLVLDREKLAAHARGASLDKAI
                     EVTSAVQIEIRVHAMRCPQCDGEYKIVDHRSSGQGARPVDVKCNMCGMPRTIWFQIVE
                     PN"
     gene            complement(2930..>3688)
                     /locus_tag="VMZ53_27750"
     CDS             complement(2930..>3688)
                     /locus_tag="VMZ53_27750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS32338.1"
                     /translation="AQCQAPGCGLILVLHGDTGNGLLMDAHVKMRDLGQQRGYIVLAP
                     TGPPRPDQGSTWTSANDATLVDMVTQFRDVFRVNPKKIHVTGFSRGGFVNWRLLCDHA
                     DLFASAAPAGAGYGGGFAETTCFQNQRAPSRKIPITFLMGRTDVSVGYTNMVNIRNAA
                     ITNYGATGPSVLASDASYTHNRWTSTDGGVIETWDHAYENYTTSAFASAQGHCIVGST
                     TDPLAPQYAIPCQPPNAFVWGEVVMDFFIANPMP"
BASE COUNT          588 a         1244 c         1186 g          670 t
ORIGIN      
        1 tacgtgctgt tgcccgacgc cgtcgacggt ctacccgcga tcgaagcgaa gctcgccgac
       61 acgatccgcg cgctgcaggg caagctcgca gaggcgcgcg tctcgctcgc gctgccgaag
      121 ttcacgatcg atccgccggg accgctcgag ctgcagcaga cgctcggcga gctcggaatc
      181 aagacggcgt tcacgccggc tgccgacttc agtggcatca cgaagaccac gaccgagccg
      241 ctgttcatct cgaatgtgat gcacaaggca ttcgtgaagg tcgacgagaa gggaaccgag
      301 gccgcggcag caaccgcgat cggcgctccg tatggtgcac cgcctgcgcc cggcactccg
      361 ttcgtcgcgg atcacccgtt cgtcttcatg atcatcgatc gatcgagcgg gctgatcctg
      421 ttcatcggcc gcgtcgccgc gccctgatca gttcgcgatc tgcgagcgct tggttttgcg
      481 ctcgagcgtc caaggaccac cgtcgctgtt cgactgcgcg tggccccacg tgccttgcag
      541 cgtgctgccc ttgatcgtcc agatgcccgc gccatgacct gcgccgcgcg gctcgtccca
      601 ctcgaacttg atgatgcggc cctcgatgat gcggccttcg atcgtgccgc cgccgcagca
      661 gacatacgta ccgtgaattc gatcgccctc ctgcacgagg tggacgtcgt cccagttgct
      721 cgcatagtcg ccggttacat cgacgcgcgc agacgcagtt gcgaggagcg gccagaagat
      781 gagggcggcg agcatgtctc cattcaacgc accagaccgc cgcgggattt gcggctaatt
      841 gtgcaggaga ttcgcgcctt ccgcgtatcg cacatacttg cgggtgacgt tccaggcgag
      901 ccacgtgccg cccgatccga cgaggaccca cggcgtgtac gcgtagaacg tcgcggtcgc
      961 gtgattgccc ggcgtgacgc gacgcgggtc gagcgtgctg cgcgagacgc ctttgcgcca
     1021 ctggccgctg ccatcgatcg aagcgtcgta ccaacgacga agcgtgcgcg cgccgcagtc
     1081 gagctgccgc tgcaagcccg agtacgccgt gttgcacgac gcgccatcgg gacagccgca
     1141 tccgagtgcg cgattgatca tgcgctcgga cggcgtcgcc gtcttcgaca cgagcgatgt
     1201 ctcgacttgc atgcgcgcga tcaagatcag cgggttcacg tcgtgcgtct gcgcggcctc
     1261 gacgaccatc tgcgcggcgg acacgccgtt gactttgtac gacgcgagcc agctgcgcgt
     1321 gccgtatgga ttcttctcga agaatgcttg cacgtccgcg acagacatcc cgccgccgtt
     1381 cgtgagcagc gcgtcatcga cgacattgtt cgcgtcgaac gtcgccggta cgccgtcggc
     1441 cttgccgtcc gccgaatcaa cgatgaagtc ttcaacctcg tcgtggtcgg cgtccggagc
     1501 ttcagcggcg catgcggtga cgacggcaat cgcgaggagc caggcggagc gcatgaccag
     1561 gcaagttgca aaggcgtgcc catgcacaag ttgatcagct ctgcgcagtt acctcgcggc
     1621 gcacatggct tctcgagttg ccgctgtctg gggctcctgc ctggggccgc agtcttcaga
     1681 gcttcgcgag cgtctcggcg agatacgtct tgatctgctc gtcgctggga atctggcact
     1741 cccccttctg cgtgagtgtc gcttcccact tccagtagcc gggcggcttc ggcggctcgg
     1801 gctgcttgcg cttccaatac cagagccgcg cttcacgcac gcccggcgga tacggctcgg
     1861 ctgctttgct gtagtcgttc gtccagagat agaagcgggg cacatagccg ctcgctgctc
     1921 cacgctcgac gatctggcgg agctcgggcg gattcttcgc gaggaagact tgcagcgcgg
     1981 tgaacgatcg ctgcgcgcag tcgacggcat cgggattcgc gctgtcgacg ttcggctccg
     2041 tctcgcccgg tgggatcacg cccttctcgg gatagaagcc ccagaggact tcctccggat
     2101 agcgaatgtc gtccttgccg acgtacggcc agtactccgc tccggcgagc gtgtagccgt
     2161 tcatctcgaa cgtgtccatg cgatcgctga gcggcgatgc ctgcggcggc ggcggcgccg
     2221 atggtggtgc tgccggcttg ttgtgcgagc aggcgatcag cagagcggcg acgagaacga
     2281 agcgacgcat gcgcggggtg tacacccagc taacgtggcg ccgtcaagga ttcccagctc
     2341 acgagcgttg ccatggcctc ctcggcggag gcgccgcaca tctcgagctg cggccggaga
     2401 ccgacgcacg tcgcaggtcg ctcgggcttg ccgaacaaca cgcagcgcag ctcgctggac
     2461 agatgcgggc agcgcacgcc cgccgccttt ccctcgggca gcgcgacgca cggcgagatc
     2521 gacggcgcga tacagcacgc cccgcagccg tctcgacagt tcatcgcgcc caccgtagca
     2581 cctcgcccgc gcgccggtga cgcctcaatt cggctcgacg atctggaacc agatcgtgcg
     2641 cggcatcccg cacatgttgc acttcacatc cacgggtcgc gcgccctgcc cgctcgagcg
     2701 gtgatcgacg atcttgtatt cgccatcgca ctgcgggcag cgcatcgcat gcacgcggat
     2761 ttctatctgc acggccgacg tcacctcgat cgctttgtcg agcgacgccc cacgcgcatg
     2821 cgctgcgagc ttctcgcggt cgaggacgag ctggcgaacc tcgcgctctt tctcgcgctt
     2881 cgcggtgcgc tccggccgcg ctggcttctt cttggacatg tcgcgccgct tacggcatcg
     2941 ggttcgcgat gaagaagtcc atcacgacct cgccccacac gaatgcgttc ggcggctggc
     3001 aaggaatcgc gtactgcggc gccaacggat ccgtcgtgct gccgacgatg cagtggccct
     3061 gcgcgctcgc gaaggcgctc gtcgtgtagt tctcgtaggc gtggtcccac gtctcgatca
     3121 cgccgccatc ggtgctcgtc caccgattgt gcgtgtagct cgcgtcgctt gcgaggacgc
     3181 taggcccggt cgcgccgtag ttcgtgatcg ccgcgttgcg gatgttcacc atgttcgtgt
     3241 agccgacgga cacgtcggtg cggcccatca agaacgtgat cggaatcttg cgcgacggcg
     3301 cgcgttgatt ctggaagcac gtcgtctcgg caaagccgcc gccatagccc gcacctgccg
     3361 gtgccgccga tgcaaacagg tccgcgtggt cgcacagcaa ccgccagttg acgaagccgc
     3421 cgcgcgagaa tccggtcacg tggatcttct tcggattgac gcggaacacg tcgcgaaact
     3481 gcgtgaccat atcgacgagc gtcgcgtcgt tcgcagacgt ccacgtcgag ccttggtcgg
     3541 gacgtggcgg ccccgtcggc gcgagcacga tgtagccgcg ctgctgcccg aggtcgcgca
     3601 tcttcacgtg cgcatccatg agcaggccgt tgcccgtatc gccgtgcagc acgaggatga
     3661 ggccgcagcc gggcgcctga cactgcgc
//
LOCUS       DATLBZ010000553         6062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4428101, whole genome shotgun sequence.
ACCESSION   DATLBZ010000553 DATLBZ010000000
VERSION     DATLBZ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4428101"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VMZ53_27755"
     CDS             complement(<1..1628)
                     /locus_tag="VMZ53_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HUS32339.1"
                     /translation="MAEQLEPHTNGNGHSTATFQRQLLATLTRFKSGDFTSRMPAELT
                     GVEGKIADSFNELLEVNERRASEIARVCRTVGKEGKLKERMRVPGAVGGWSDEIDALN
                     TLIGDLVWPTTEVTRAVGAVAKGDLSQSMSLEVDGRPLEGEFLRSAQLVNKMIDQLSV
                     FTSEVTRVAREVGTEGKLGGQAQVKGVSGVWKDLTESVNQMAGNLTAQVRNIAEVTIA
                     VASGDLSKKITVDVRGEILQLKEAINTMVEQLRSFASEVTRVAREVGTEGKLGGQAVV
                     PGVAGTWKDLTDNVNSMASNLTGQVRNIAEVTTAVARGDLSRKITVDVRGEILELKNT
                     INTMVDQLNGFASEVSRVAREVGTEGKLGGQALVPGVAGTWKDLTDNVNSMASNLTGQ
                     VRNIAEVTTAVARGDLSRKITVDVRGEILELKNTINTMVDQLNGFASEVSRVAREVGT
                     EGKLGGQAVVPGVAGTWKDLTDNVNAMASNLTGQVRNIAEVTTAVAKGDLSRKITVDV
                     KGEILELKNTINTMVDQLNAFASEVSRVAREVGT"
     gene            1952..2992
                     /locus_tag="VMZ53_27760"
     CDS             1952..2992
                     /locus_tag="VMZ53_27760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32340.1"
                     /translation="MTGRHTVLQAIPASSSRVAIGDLDGDHRNEIVACGPDALTVLDA
                     SGKKLAQLTIVDGIQKLVVADLDSDGSAEIYAGWGQTREHMDTKARFSVLHFGAGALR
                     EENIVTPATTRQDVVSIVPVFEDRTMYLMYFESKYIVASVRVWRDRQTWIVQEAPPTF
                     RMASSFARGDVDADGYQELVIGRVYGDDVGLDGDASVNTSRRGLTMTIPTTRGVRSAA
                     VIDGAVYLGDGWHKNYGTEARGLFSRATFDVSTNAFVTKIIEDTPGQYAIEAIIPATI
                     ENRRVIVTQGTKYTRAFRPNGDKWEAVTFAGLARDVAVGDLDGKPGDEILILGETAEI
                     VSLEGATWSPIP"
     gene            2989..4242
                     /locus_tag="VMZ53_27765"
     CDS             2989..4242
                     /locus_tag="VMZ53_27765"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS32341.1"
                     /translation="MTSWSALVVDDDAGIRQSLRLCLEADDARVLGVGSAPAALEALD
                     RARFDVVVLDLWLGKESGLDAIPALLERQRDVAIVVVTAFATFESAVEAMKRGAFDYL
                     PKPFTPDQVRAATRRALEAVRLKHRLADAEHRLEQAGASDERFETESASFRRVLDQAA
                     RAAVADAPILLRGESGTGKNVLARFIWQQSQLKNGPYVTLSGTGDDIVAKIQQARGGT
                     LAVDELGELSPDAQARLLRSLDEKDIRIIAATNRNIDDEIRAGRIREDLLFRLKVVTL
                     TLPPLRERSEDIMPLAQHFLARAATRQHRPAFEFSESALRAVGSYNWPGNLRELRNAV
                     ERAVILSPGPRIEVDDLGIPTAPSPMSSNGIEVALGADVSIDQLEREHIARIIARAPT
                     LEAAARTLGIDASTLQRKRKRYGLT"
     gene            4239..5996
                     /locus_tag="VMZ53_27770"
     CDS             4239..5996
                     /locus_tag="VMZ53_27770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS32342.1"
                     /translation="MSTLQLRTRLAIAGSLLFATTLAIGLWSATAFRRVSQVVSDTVT
                     ANQRITDATTTLTSALEREDDVVVLVLDDAPGAYSQLGVQRELVAAALARIEDPALRA
                     DVEKYETAVDELISGKHGPLALENYREVVNPLLRGAETTIEHIRDDHFASSQHVAEWA
                     GVQSTRSMQIVATISGVALLMLLLVVIHFARVVVAPIVEMTRVVQAIRRGDFSQRVTS
                     RRDDEIGRLGIGLSRMADELEEFKRTNIGEVIHAKETLEATLDAFPDAVLVIDGAKNV
                     SAANPRAQAVLPLPDGVLETSNSALETVDLTKTIEVTLSGQQRRLLPRMVPIADGRGA
                     VLVLSDVTELARLDERRLELVAVASHELRTPLTTMRMTLSMLHEHATQYHARDRELIT
                     TAMLGVDQLAALVDEFLDLTRIEAGQLRLHWTTVVARDLIASAVKTAAPSAEQASVAL
                     EVDHAPSTPDEVPGDPARIAMVLSNLLTNAIKYTPAGGRVQVRTSGAPGELIVEVSDN
                     GPGIPAELRERVFERFFRVEHAQKSAPPSAGVGIGLYIARQVIELHGGTIRCDTGPLG
                     GARFTMTIGKLPPGATAEG"
BASE COUNT         1072 a         1998 c         1958 g         1034 t
ORIGIN      
        1 tcggtgccga cctcgcgcgc gacgcggctc acctcggagg cgaacgcgtt cagctgatcg
       61 accatcgtgt tgatcgtgtt cttcagctcg aggatctcgc ccttcacgtc gacggtgatc
      121 ttgcgcgaca ggtcgccctt cgcgacggcg gtggtcacct cggcgatgtt gcgtacctga
      181 cccgtcaggt tcgacgccat cgcgttgacg ttgtcggtga ggtccttcca cgtgccggcg
      241 acaccgggga cgacggcctg gccaccgagc ttgccttcgg tgccgacctc gcgagcgacg
      301 cgcgatacct ccgacgcgaa gccgttcagc tgatcgacca tcgtgttgat cgtgttcttc
      361 agctcgagga tctcgccgcg aacgtcgacc gtgatcttgc gcgacagatc gccacgcgcg
      421 accgcggtgg tcacctcggc gatgttgcgg acctgaccgg tcaggttaga cgccatcgag
      481 ttgacgttgt cggtgaggtc tttccacgtg ccggcaacgc cggggacgag cgcctgaccg
      541 ccgagcttgc cttcggtgcc gacctcgcga gcaacgcgcg acacttccga cgcgaagccg
      601 ttcagctgat cgaccatcgt gttgatcgtg ttcttcagct cgaggatctc gccgcggacg
      661 tcgaccgtga tcttgcgcga tagatcgcca cgcgcgacgg cagtcgtcac ctcggcgatg
      721 ttgcggacct gacccgtcag gttcgacgcc atcgagttga cgttgtcggt gaggtctttc
      781 cacgtgccag cgacgccggg gacgacggcc tgaccgccga gcttgccctc ggtgccgact
      841 tcgcgagcca cgcgcgtcac ttccgatgcg aacgagcgga gctgctcgac catcgtgttg
      901 atcgcttctt tgagctggag aatttcgccg cgaacgtcga cggtgatctt cttcgacaag
      961 tcacccgacg cgacggcgat cgtaacttca gcaatgtttc gaacctgcgc ggtgaggtta
     1021 ccggccatct ggttcacgga ctccgtgagg tccttccaga cgcccgacac gcccttcacc
     1081 tgcgcctgac cgccgagctt tccttcggta ccgacttcgc gagcaacgcg cgtcacttcc
     1141 gaggtgaaga cggacagctg atcgatcatc ttgttgacga gctgcgcgga gcgaaggaac
     1201 tcgccttcga gcgggcggcc gtcgacttca agcgacatcg attgcgagag atcgcctttg
     1261 gcgacggcac cgacggcgcg cgtcacctcc gtggtcggcc acacgaggtc gccgatgagc
     1321 gtgttgagcg cgtcgatctc gtcggaccat ccaccgacgg caccgggcac gcgcatacgc
     1381 tctttgagct ttccttcttt accgaccgtg cggcacacac gcgcgatctc actcgcacgg
     1441 cgctcgttga cttcgagcag ctcgttgaac gagtcggcga tcttgccttc gacgcccgtg
     1501 agctccgccg gcatgcgtga cgtgaagtcg cccgacttga atcgcgtgag cgtcgcgagc
     1561 agctgacgtt ggaacgtcgc ggtcgaatga ccattgccgt tggtatgcgg ctccagttgc
     1621 tctgccactt gatccggagt cagtagcgtt ggcgcagact ccagcgtgcg cggcggacga
     1681 tccgtcggtt cagtcatcgt tcgtaagctc ccctcacgac ctatcgaaga gatcgcgact
     1741 ccagggactt accatagtta gcgggtagcc ccacgaagaa acgtagaagt tccgcacgct
     1801 tgaaatgcgc gcctacgcgc gctgtagcgc gagcgctcgc tcccggttag gcccgacctc
     1861 tgatactgag agagcgttgc gccaggtcct cgggatcatt ttgctctggg gcgcctgtca
     1921 atcaaccgac aggacgccca cgggatcgcc gatgaccggc aggcacactg tgttgcaagc
     1981 gattcctgcg agctcttcgc gcgtcgcgat cggggacctc gatggcgatc atcgcaacga
     2041 gatcgtggcg tgcggtccgg acgcgctgac ggttctcgat gcgagcggca agaagctcgc
     2101 gcagctcacg atcgtcgatg gcattcagaa gctcgtcgtc gcggatctcg atagcgacgg
     2161 ctccgcggag atctacgcgg ggtggggaca gacgcgcgag cacatggata cgaaggcgcg
     2221 cttcagtgtc ctgcatttcg gcgcgggcgc gctgcgcgaa gagaacatcg tgacgcccgc
     2281 gacaacgcgt caggacgtgg tgtccatcgt gccggtgttc gaggacagga cgatgtatct
     2341 gatgtacttc gaatcgaagt acatcgtcgc gagcgtccgc gtgtggcgcg acagacagac
     2401 gtggatcgtt caagaggcac cgccgacgtt ccgaatggca tcgagcttcg cgcgcggaga
     2461 cgtcgacgcc gatggctacc aggagctcgt gatcgggcgc gtgtacggtg acgacgtcgg
     2521 cctcgacggg gatgcgtccg taaacacgag tcgtcgagga ctaaccatga caattccaac
     2581 gacgcgcggt gtgcggtccg ccgcagtgat cgatggcgcg gtgtatctcg gcgatggctg
     2641 gcacaagaac tacggcaccg aagcccgtgg gctcttcagt cgcgcgacgt tcgacgtctc
     2701 gacgaatgcc ttcgtcacga agatcatcga ggacacgccc ggccagtacg cgatcgaagc
     2761 aattattccc gcgacgatcg agaatcgccg cgtgatcgtc acgcagggca cgaagtacac
     2821 ccgggcattt cgcccgaacg gcgataaatg ggaggccgtg accttcgccg gccttgctcg
     2881 tgacgtggcg gtgggcgatc tcgacggtaa gcctggagac gagatcctga tcctcggcga
     2941 gacagccgag atcgtgagcc tcgaaggcgc aacctggagc cccatcccat gacgagctgg
     3001 tcagcacttg tcgtcgacga cgacgccggt atccggcaat ctctccgcct ctgcctcgaa
     3061 gccgacgacg cgcgcgtcct cggtgtgggc agtgcacccg cggcactcga ggcgctcgat
     3121 cgtgcgcgct tcgatgtcgt cgtgctcgat ctgtggctcg gcaaagagtc ggggctcgac
     3181 gcgattccag cgctgctcga acgccagcgt gacgttgcga tcgtcgtcgt gaccgcattc
     3241 gcgacgttcg agtccgccgt cgaagcgatg aagcgcggcg cgttcgatta cctgccgaag
     3301 ccgttcacgc cggatcaagt gcgcgcggcg acgcgacgag cgctcgaagc ggtccgcctc
     3361 aagcatcgcc tcgccgacgc cgagcatcgc ctcgagcaag caggcgccag cgacgagcgc
     3421 ttcgagaccg agagcgcatc gtttcgtcgg gtgctcgacc aagcggcacg tgcggcggtc
     3481 gcggacgcac cgatcctgct gcgcggcgag agcggcactg gcaagaacgt gctcgcgcgc
     3541 ttcatctggc agcagagcca gctgaagaac ggcccgtacg tcacgctgtc cggaactggc
     3601 gacgacatcg tcgcgaagat ccagcaagcg cgcggcggca cgctcgcggt cgacgagctc
     3661 ggcgagctgt cacccgatgc gcaagcgcgg ctgttgcggt cgctcgacga gaaagacatc
     3721 cgcatcatcg cggcgacgaa ccgcaacatc gacgacgaaa tccgcgcggg tcgcattcgc
     3781 gaagacctgc tgttccggct aaaggttgtc acgctcactc ttccgccact tcgcgagcgc
     3841 agcgaggaca tcatgcctct cgcgcagcac ttcctcgcgc gagcagcgac gcggcagcat
     3901 cgcccggcgt tcgaattctc ggagagcgcg ctgcgtgcgg tcggttcgta caactggcca
     3961 ggcaacttgc gcgagctccg caatgctgtc gagcgcgccg tgatcctcag cccgggcccg
     4021 cgcatcgaag tcgatgatct cggcatcccg acggcgccgt caccgatgtc cagcaacggg
     4081 atcgaggtag cgcttggcgc ggatgtcagc atcgaccaac tcgagcgcga gcacatcgct
     4141 cgcatcatcg cgcgcgctcc gacgctcgaa gcagctgcgc gcacgctcgg catcgacgcg
     4201 tcgacgctac agcgcaagcg aaagcgttac ggcctgacgt gagcacactg cagctccgca
     4261 cccggctcgc gatcgcagga tcgctgctct tcgcgacgac gctcgcgatc gggttgtgga
     4321 gcgcaaccgc cttccgtcga gtcagccaag tcgtcagcga cacggttaca gcgaaccagc
     4381 ggatcaccga cgcgacgacg acgctgacaa gtgcgctcga acgcgaagac gacgtcgtcg
     4441 tgctcgtgct cgacgatgca ccgggtgcgt acagccagct cggggtgcag cgagagctgg
     4501 tggccgcagc gctcgctcgc atcgaagatc cagcgctgcg cgccgacgtc gagaagtacg
     4561 agaccgcggt cgacgagctg atctccggca agcacggtcc gctcgcgctc gagaactacc
     4621 gcgaggtggt gaacccgctg ctccgcggcg cggagacgac gatcgagcac attcgcgatg
     4681 atcacttcgc atcgagccaa cacgtggcgg agtgggcagg cgtgcaatcg acgcgctcga
     4741 tgcagatcgt cgcaacgatc agcggagtgg cgctgctcat gctgctgctc gtcgtcattc
     4801 acttcgcgcg cgtggtggtc gcaccgatcg tcgagatgac gcgcgtcgtc caggcgatcc
     4861 gccgcggcga tttctcgcag cgcgtgacga gccgccgcga cgacgaaatc ggccgcctcg
     4921 gcatcggcct ctcgcgaatg gccgacgagc tcgaagagtt caagcgcacg aacatcggcg
     4981 aggtgatcca cgcgaaggag acgctcgaag caacgctcga tgcattcccc gacgcagtcc
     5041 tcgtcatcga cggcgcgaag aacgtgtcgg ccgcgaatcc gcgcgcgcag gccgtcctgc
     5101 cgctgcccga tggtgtgctc gagacgagca acagcgcgct cgagaccgtc gacctgacga
     5161 agacgatcga ggtcacgctg tcgggccagc agcggcgcct cctccctcgc atggtgccga
     5221 tcgccgatgg ccgcggagcg gtgctcgtgc tctccgacgt caccgagctg gcgcgcctcg
     5281 acgagcgtcg tctcgagctg gtcgccgtcg catcacacga gctgcgcacg ccgctcacga
     5341 cgatgcgcat gacgctgtcg atgctgcacg agcacgcgac gcagtatcac gcgcgggatc
     5401 gcgagctcat cacgacggcg atgctgggtg tcgatcagct cgccgcgctc gtcgacgagt
     5461 tcctcgatct cacgcgcatc gaggccggtc agctgcggct gcactggacg actgtcgtcg
     5521 cacgagacct gatcgctagc gcggtcaaga ccgcggcacc ctcggccgag caagcgagcg
     5581 ttgcgctcga ggtcgaccac gccccgtcga cacccgacga agttcccggc gatccggcgc
     5641 gcatcgcgat ggtgctttcg aacttgctga cgaacgcgat caagtacacg cccgccggcg
     5701 gtcgcgtgca ggtccgcacc tccggcgcac cgggcgagct catcgtcgag gtcagcgaca
     5761 acggccccgg cattccggcc gagctgcgcg agcgcgtgtt cgagaggttt ttccgcgtcg
     5821 agcacgcgca gaagtctgca ccgccctccg caggcgtcgg catcggtctc tacatcgcgc
     5881 gccaagtgat cgagctgcac ggcggcacga tccgctgcga caccggtccg ctcggcggcg
     5941 cgcggttcac catgacgatc ggcaagctgc cgccaggcgc gaccgctgaa ggctagagca
     6001 gcttgttgcg ccgcatcatg tagagcaggc cgaacaacaa caggagcacg agcccccaga
     6061 ag
//
LOCUS       DATLBZ010000554         6457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2965014, whole genome shotgun sequence.
ACCESSION   DATLBZ010000554 DATLBZ010000000
VERSION     DATLBZ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965014"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VMZ53_27775"
     CDS             complement(<1..437)
                     /locus_tag="VMZ53_27775"
                     /inference="COORDINATES: protein motif:HMM:NF023234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase domain-containing protein"
                     /protein_id="HUS32343.1"
                     /translation="MKGASILALEAGPRSREAVDAFFATQKFPIVEGNFITFVWRGEA
                     EAVHLKHWVFGLPSSQQLARVEKTDVWYLTLQLPPGSRVEYKLEVVRNGHGEWMQDPL
                     NQNTARDPFGGNSVAHGTGYEIPSWIHPDPTAAKGHRDEIWIDS"
     gene            complement(434..1405)
                     /locus_tag="VMZ53_27780"
     CDS             complement(434..1405)
                     /locus_tag="VMZ53_27780"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HUS32344.1"
                     /translation="MAPVVLLGPQGTQPTAGGVLAELGVRGKVALVRAGYQERESEDA
                     DMVASLGVPAVNLKLHARSIEVFREDTELTTAYSARQQRLRHIQAFYRVRLDKIEDAA
                     KNISVRYVEGELLEMEEKVTVDQFRHIDADHIERCNAVRYEFDKNWRFVDRPVIARHR
                     EELRKELATCDALVIAGGHVASLLNRLAMFGVLELAAGKPIVAWSAGAMVLTERIVLF
                     HDYPPYGSDIAQVLDAGFAIAPGLVVLPDPHRRVNFDAKAGIQRFARRMAPATCVAMD
                     HGSRVVFEGDKIVRANGVRLTTTGAVDLDWNGSPTRFSNPFLRGVPL"
     gene            complement(1405..2622)
                     /locus_tag="VMZ53_27785"
     CDS             complement(1405..2622)
                     /locus_tag="VMZ53_27785"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS32345.1"
                     /translation="MVHIVFIEPRFPANQKQFIKGLAEIGATISAIGEGSKDSLDDDL
                     RRWLTHYEEVTNVCDEAQVLNAVRFIQRHKHVDRLEATVEAHIMPCAHVREKAGIPGT
                     STRTAFLCRDKPAMKQVLREGGVPCAESTAAASADEVRAFIERVGYPVILKPRDGAGA
                     SGASRVESDRELEPALRALGPGRSIAVEEFIEGHEGFFDTLTLRGQVVHEFATHYYPN
                     VLEAMRTRWISPQFITTNRINTPGYDEVKQMAQRVVTLLGIETSATHMEWFSGPKGLK
                     FSEIGCRPPGVRAWDLYNVANDMDLYREWAMLIAAGKPGQYPSRRLSAGIIAIRPDRD
                     GRISHYEGLQAVREAFGEFLIDWHLPPEGTPTQGVEAGYMANAWIRLKHENYDHLRHM
                     LDVVGQTVKVRAH"
     gene            complement(2626..4068)
                     /locus_tag="VMZ53_27790"
     CDS             complement(2626..4068)
                     /locus_tag="VMZ53_27790"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HUS32346.1"
                     /translation="MNVLFVASEMAPFAKTGGLADVMAALPPYLARRGHDVRVVLPLY
                     DTVDTSKATFQAIMDFELPLGTHRYQLKIFQVGSGPRLYFVHCPALYSRGRLYTSDPD
                     EHRRFLALSYAAMIICQRLDFAPDIIHCHDWQAALLPMMVKTLFANDPRFAKTRSLLT
                     IHNLMYQGSFPVEIGPDTNLTSVAHLFHQDLMKAGRVNFLLQGILYADGVSTVSPTYA
                     KEIQTTTYGGGLDGYLRARSATVVGVLNGVDYDEWSPERDRFIPHKYSAQDLAGKERD
                     KEHLLTSVGLPYVKGVPVLGIVSRLVGQKGFKLLDPTMFELIGKHGFQLVVLGSGEPA
                     LEDMFGHMQRAYPRQVCFYQGFKNDLAHLIEAGADMFVMPSVYEPCGLNQLYSLRYGT
                     VPIVHRTGGLADTVTQWNPRTGDGTGFVFANHDAAGLRWAVEAALAAYRDPAQWNRLI
                     QNGMAQDFSWEAQGKLYELLYARLASLGRH"
     gene            complement(4080..4982)
                     /locus_tag="VMZ53_27795"
     CDS             complement(4080..4982)
                     /locus_tag="VMZ53_27795"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HUS32347.1"
                     /translation="MVALVSIPTILDVDVIRGARAAQGAPLDVVIEIPHGATRTEDFN
                     ALAARVTSPLPDGLVDFFHVNTDAGAPELAVATARRFVELEPARSVAILRCRIPRTFI
                     DCNRRVDASAEDFKAGKVSPGLMPWITDPEDRVMLRAAYDRYVAAVKQATGQLATDGA
                     MVLLHSYAPREVDVQVDAKIVESLRAAYRPEKVESWPLRPELDVIGKGLDGTSYAPAA
                     VVEALKAALAGELAVADGATYPLHSSTMAWDHVMARPGRTLCLEVRRDLLADPFEPFA
                     QMRISEAKVERIAVPLATALRRWW"
     gene            5068..5907
                     /locus_tag="VMZ53_27800"
     CDS             5068..5907
                     /locus_tag="VMZ53_27800"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS32348.1"
                     /translation="MRGSCLLLAVATLITSAQASADTTDFGFEVEQLFGYDADPPPKF
                     AFSNGDFYTAKVRRPSIGGVQIGETSVASKTVVATSKDGSVAWVASDVTVGCVEQAGD
                     ECMAGIGTPLHATGVLLKAGKDWQWIAWHFAEVTTAKEQAALVKNGVVPGAIPRSVTG
                     AEDVVAVFEKSFADPKELAASVSTRKDVVLYGSSVAERVVGGAKVKAKLKGWNLSFKV
                     RDGIQAGLVGTTVGWVAANVDAAAAKGKPATSSPYRVLAVYEKSGSAWKLVQVHFSVD
                     QTK"
     gene            6079..>6457
                     /locus_tag="VMZ53_27805"
     CDS             6079..>6457
                     /locus_tag="VMZ53_27805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32349.1"
                     /translation="MATPISRALRWLVRPLKPFVVALIPDRRVGPEVVAGRYGWPLLS
                     VILCACIAAFALGTRLDVAPDVRAENAGAATVEANSKSKPAPEIKTDREIDEAIAQRA
                     AVVRVKLGLGAALGTPFRILALAL"
BASE COUNT         1115 a         2108 c         2120 g         1114 t
ORIGIN      
        1 gagtcgatcc agatttcgtc gcgatggccc ttcgcagcgg tcgggtcggg gtggatccag
       61 cttgggatct cgtagcccgt tccgtgcgcg acggagttgc caccgaacgg atcgcgcgcg
      121 gtgttttggt tgagcggatc ctgcatccac tcgccgtgtc cgttgcgaac gacctcgagc
      181 ttgtactcga cgcgcgagcc cggcggcagc tgcagcgtga ggtaccagac gtcggtcttc
      241 tcgacgcgtg cgagctgctg cgacgacggc aggccgaaca cccagtgctt gagatgcacg
      301 gcctcggctt cgccgcgcca cacgaacgtg atgaagtttc cctcgacgat cggaaacttc
      361 tgcgtcgcga aaaatgcatc gaccgcctcg cggctgcgcg gaccggcctc gagcgccaga
      421 atcgacgcac ccttcacagc ggcactccgc gcaagaacgg attggagaac cgcgtgggcg
      481 aaccgttcca gtcgaggtcg acggcaccgg tcgtcgtcag ccgcacgccg ttcgcgcgaa
      541 cgatcttgtc gccttcgaac acgacgcgcg agccgtgatc catcgcgacg cacgttgcgg
      601 gcgccatccg ccgcgcgaac cgctggatgc ccgcctttgc gtcgaagttc acgcgacggt
      661 gcggatccgg cagcacgacg aggcccggcg cgatcgcgaa gcccgcgtcg aggacctgcg
      721 cgatatcgga gccgtacggc ggataatcgt ggaacagcac gatgcgctcg gtgagcacca
      781 tcgcgccggc cgaccacgcg acgatcggct tgccggcggc gagctcgagg acgccgaaca
      841 tcgcgagccg gttgagcagc gacgccacgt gaccacccgc gatgacgagc gcatcacacg
      901 tcgcgagctc cttgcgcagc tcttcgcgat gccgcgcgat gaccggccgg tcgacgaagc
      961 gccagttctt gtcgaactcg tagcgcactg cattgcagcg ctcgatgtga tcggcgtcga
     1021 tgtggcggaa ctggtcgacg gtgaccttct cctccatctc gaggagctcg ccttcgacgt
     1081 agcgcaccga gatgttcttc gctgcgtcct cgatcttgtc gaggcgcact cgatagaacg
     1141 cctggatgtg gcgaagccgc tgctggcgcg cggagtatgc agtcgtgagc tcggtgtcct
     1201 cgcggaacac ttcgatgctg cgcgcgtgga gcttgagatt cacggcagga acgccgagcg
     1261 acgcgaccat gtcggcgtct tcggactcgc gctcctgata gcccgctcgg acgagcgcga
     1321 ccttgccgcg cacgccaagc tccgcgagca cgccaccggc cgtcggttgc gttccctgcg
     1381 gaccgagcag gacaacaggc gccatcagtg cgctcgaacc ttcaccgttt gcccgacgac
     1441 gtcgagcatg tggcggagat gatcgtagtt ctcgtgcttg agccggatcc acgcgttcgc
     1501 catgtagccg gcttcgacgc cttgggtcgg cgtgccctcg ggcggcaaat gccagtcgat
     1561 gaggaactcg ccaaacgcct cgcgcaccgc ctgcaggccc tcgtagtgcg aaatcctgcc
     1621 gtcgcgatcg ggacgaatcg cgatgattcc cgcggacagt cggcgcgatg ggtactgacc
     1681 gggcttgcct gccgcgatga gcatggccca ttcgcgatac aggtccatgt cgttcgcgac
     1741 gttgtagagg tcccacgcgc gtacgcccgg tgggcggcaa ccgatctccg aaaacttcag
     1801 ccccttcggt ccggagaacc actccatgtg cgtcgcggag gtctcgatgc cgagcagcgt
     1861 gaccacgcgc tgggccatct gcttcacctc gtcgtagccg ggcgtgttga tccggttcgt
     1921 cgtgatgaac tgcggcgaga tccaacgcgt ccgcatcgcc tcgaggacgt ttggataata
     1981 gtgcgtcgcg aactcgtgca cgacttgccc gcgcagcgtc agcgtatcga agaagccttc
     2041 gtgaccttcg atgaactcct cgacggcgat cgagcgtccc ggtccaagcg cgcgcagcgc
     2101 cggctcgagc tcccgatcac tctcgacgcg tgacgcgccg gacgcacccg caccgtcgcg
     2161 cggcttcaag atcaccgggt agccgacgcg ctcgatgaac gcgcggactt catccgccga
     2221 cgccgccgcg gtcgactccg cgcacggcac gccgccttcg cgcagcacct gcttcattgc
     2281 cggcttgtcg cgacacagaa atgcagtccg cgtgctcgtc cccgggatgc ccgccttctc
     2341 gcgcacgtga gcgcacggca tgatgtgggc ctcgacggtc gcttcgagcc ggtcgacgtg
     2401 cttgtggcgc tggatgaagc gcaccgcatt gaggacctgc gcctcgtcgc agacgttcgt
     2461 gacttcttcg tagtgcgtga gccaccggcg gagatcgtcg tcgagcgaat ctttcgagcc
     2521 ttcgccgatc gcgctgatcg tcgcgccgat ttcggcgagg cccttgatga actgcttctg
     2581 gttcgcaggg aaccgcggct cgataaagac gatgtgcacc atgattcaat gccgtccgag
     2641 cgatgcgaga cgcgcataga gtagctcgta gagcttgccc tgggcttccc acgagaagtc
     2701 ctgcgccatg ccgttctgga tcaaccggtt ccattgcgcc ggatcgcgat atgcggcgag
     2761 cgccgcctcg acggcccacc gcaagcccgc ggcatcgtga ttcgcgaaca cgaagcccgt
     2821 accgtcgcca gtccgcgggt tccactgcgt gacggtatcg gcgagaccgc cggtgcgatg
     2881 cacgatcggc accgtgccat agcgcaggct gtacagctga ttgagcccac acggctcgta
     2941 caccgacggc atcacgaaca tgtcggcgcc ggcctcgatc agatgcgcga ggtcattctt
     3001 gaagccttgg tagaagcaga cttggcgcgg atacgcgcgc tgcatgtggc cgaacatgtc
     3061 ctcgagcgcc ggctcgccgc tgccgagcac gacgagctga aagccgtgct tgccgatcag
     3121 ctcgaacatc gtcggatcga gcagcttgaa gcccttctgc ccgacgagtc gcgacacgat
     3181 accgagcacg ggcacgccct tgacgtacgg cagtccgacg ctcgtcagca gatgctcttt
     3241 gtcgcgttct ttgcccgcga gatcctgcgc gctgtatttg tgcgggatga agcggtcgcg
     3301 ctcgggtgac cactcgtcgt agtcgacgcc gttcaaaacg ccgacgaccg tggccgaacg
     3361 cgcgcgcaga tagccatcga gaccgccgcc gtacgtcgtc gtctggatct ctttggcgta
     3421 cgtcgggctc accgtgctga cgccatcggc gtacaggatg ccctgcaaga gaaagttcac
     3481 gcggcccgcc ttcatcagat cctgatgaaa cagatgcgcg accgacgtca ggtttgtatc
     3541 gggcccgatc tcgacgggaa agctaccctg atacatcagg ttgtgaatcg tcagcagcga
     3601 gcgcgtcttc gcgaaccgcg gatcgttcgc gaacagcgtc ttcaccatca tcggcaagag
     3661 cgccgcctgc cagtcgtggc agtggatgat gtcaggcgcg aaatcgagcc gctggcagat
     3721 gatcatcgcc gcgtacgaca gcgcgagaaa ccggcgatgc tcgtccggat cgctcgtgta
     3781 gagccggccg cgcgagtaca gcgccgggca gtgtacgaag tacaggcgcg ggccgctgcc
     3841 gacctgaaaa atcttgagct ggtagcgatg cgtcccgagc ggcagctcga agtccatgat
     3901 cgcttgaaac gtcgccttcg acgtgtcgac cgtgtcgtag agcggcagca cgacgcgaac
     3961 gtcgtgacca cggcgcgcga ggtacggcgg tagcgcggcc atcacatccg cgagaccacc
     4021 cgtcttggca aagggcgcca tttcggacgc gacgaacaac acgttcacgg caccaccgtc
     4081 taccaccagc gacgtagcgc tgtcgctagc ggaactgcga tgcgttcgac cttggcctca
     4141 gagattctca tctgggcgaa cggctcgaat ggatcggcca ggaggtcgcg cctcacctcg
     4201 aggcacaggg tccggcccgg gcgtgccatg acgtggtccc acgccatcgt gctcgaatga
     4261 aggggatagg tcgcgccatc ggcgacggcg agctcgccgg ccagcgcagc tttcagcgcc
     4321 tcgaccacgg ccgccggcgc atagctcgtg ccatcgagcc ccttgccgat gacgtccagc
     4381 tcgggccgga gcggccagct ctcgactttc tcgggccgat acgcggcgcg caagctctcg
     4441 acgatcttgg cgtcgacctg gacgtcgact tcgcgaggcg catagctgtg gaggaggacc
     4501 atcgcgccgt cggtcgcgag ctggccggtc gcctgtttga ccgccgcgac atagcggtcg
     4561 tacgccgccc gcaacatcac gcgatcctcc ggatccgtga tccaaggcat cagtcccggg
     4621 ctgaccttgc ctgccttgaa gtcctcggcc gacgcatcga cccggcggtt gcagtcgata
     4681 aaggttcgcg ggattcggca tctgaggatc gcgacgctgc gcgcaggctc tagctcgacg
     4741 aaccgccgcg ccgtggccac cgcgagctcc ggtgctccgg cgtccgtgtt gacatgaaag
     4801 aagtcgacga ggccatcggg cagcgggctc gtcacgcgcg cggcgagcgc gttgaagtcc
     4861 tcggtccgcg tcgcgccgtg cgggatttcg atcacgacgt cgagtggagc gccttgcgct
     4921 gcgcgcgccc cgcggatgac atcgacatcg aggatcgtcg ggatcgagac cagcgcgacc
     4981 atgggccgac catgccactt accggccccg cgtgggagcg cgagagacgc agctcgcact
     5041 cgacgccgca gcgtgtacgc tagatggatg cgcggttcct gtttgctcct ggcggtcgcc
     5101 actctcatca catcggcgca ggcgtcggcg gacacgaccg atttcgggtt cgaggtcgaa
     5161 cagctgttcg gctatgacgc cgatccgccc ccgaagttcg cgttcagcaa cggcgatttc
     5221 tacacggcga aggttcggcg acccagcatc ggcggcgtac agatcggtga gacatcggtc
     5281 gcctcgaaga ccgtcgtcgc aacgtcgaaa gacggcagcg tcgcctgggt cgcgagcgac
     5341 gtcaccgtcg gctgtgtcga gcaagcgggc gacgaatgca tggcggggat cgggacgcca
     5401 ctgcacgcga ccggcgtgct gctgaaggcg ggtaaggact ggcagtggat cgcgtggcac
     5461 tttgcggagg tcaccacggc gaaggagcaa gcggctctcg tgaagaacgg cgtggtccct
     5521 ggcgcgattc cgcgctcggt taccggagcc gaagatgtcg tcgcggtatt cgagaaatcg
     5581 ttcgccgatc ccaaggagct cgcagcgagc gtatcgacac gcaaagacgt cgtgctgtac
     5641 ggcagctccg ttgccgagcg cgtggtcggc ggagcgaaag tcaaagcgaa gctgaagggc
     5701 tggaatctct cgttcaaggt acgtgatgga atccaggccg ggctcgtcgg tacgaccgtc
     5761 ggctgggtcg ccgcgaacgt cgacgccgcc gccgccaagg gcaagccagc aacgtcgtcg
     5821 ccgtatcgcg tgctcgccgt ctacgaaaaa tcggggagcg cgtggaagct cgtgcaggtc
     5881 cacttctccg tcgatcaaac gaagtgagct gttcgagaag tcgcggggcg tcgcagcggc
     5941 gtcgcagtcg caggcagcgc aagcgaacac ccgcctaatc gcgctagcca ggcgtagtcg
     6001 ccgctagagt tgtggcacgc tcgctgcaac ttcgggctcg ccttgaacga cttgagtgat
     6061 ctgcgtgcga cgccacccgt ggcgaccccg atcagccggg cgctgcgctg gcttgtgcgt
     6121 ccgctgaagc cattcgtcgt ggcgctgatt ccagatcgac gtgtcggtcc ggaagtcgtc
     6181 gcaggtcggt acggatggcc actgctcagc gtgatcttgt gcgcgtgcat cgctgcgttt
     6241 gcgctcggga cgcgcctcga tgttgcaccc gacgttcgtg cggagaacgc gggcgctgcg
     6301 accgtcgagg cgaacagcaa gtcgaagccg gcgcccgaga tcaagactga ccgcgagatc
     6361 gacgaagcga tcgcgcagcg cgcggcagtc gtgcgcgtca agctcggact cggcgccgcg
     6421 ttggggacgc cgtttcgcat cctcgcgctc gccttgg
//
LOCUS       DATLBZ010000555         3400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7811981, whole genome shotgun sequence.
ACCESSION   DATLBZ010000555 DATLBZ010000000
VERSION     DATLBZ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7811981"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VMZ53_27810"
     CDS             <1..936
                     /locus_tag="VMZ53_27810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32350.1"
                     /translation="IGQRFPQATCVVDAGAVRIELAIDGVTYRARAQMEPRGRVVVQL
                     PSTDGFELALSWTDRAELVTGTPTFDDSCLVETNDVSLANMWLDAEARAALLASRYVS
                     GTPQALRTTMPLLRDGAWQHEVRSDEVCAQRADAEDSAERIVDMLAASLALARRPARW
                     ARWFAPLAKSLGGEASARVELGGRPVLRVRRGSSDVSIRLLRRLGPGDVGRLRTVISA
                     HRVASSGEVLTLIAEDLPRSAWPPANDHTESTLRIDARARTLLDSARPSTTIVRRHDV
                     EITFDGALADRDRLGAAVELAAYWATDHSHAGPYR"
     gene            949..2085
                     /locus_tag="VMZ53_27815"
     CDS             949..2085
                     /locus_tag="VMZ53_27815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32351.1"
                     /translation="MRMAVVWLLALCACESRYGDYFNVDGDSNGIRFDHVELYFGKHG
                     MSDSNIGKPSGSTTGDVFIRDADASDAWDVDKNGDGSLASESTYWLPITEQNRELAYV
                     AALAYDTTMQSDKPVGVGEVLGFALEDGIVNKYDIELQKFDFTVDTWGQSPGCFAWTR
                     QRDGKFSTIAVLSPDDADCDGVVASADCNDLCPAGSTKCNPDETVCGGANSCGLGCAI
                     NGLCRIETCLPTIACTNCGMFAASLDDRMKCVSSDPAHHLSVLVDTLGGKPCARSFDV
                     PNYGRPCSGVTEVWQQNFGDGWDLTVSPSANDLTHCIVELTNTSTSATFAGDHHVLVA
                     FPPRAPSSSQWSVLLGVKDVPATACQIDGGYTIDGNVGAPNDCL"
     gene            2166..>3400
                     /locus_tag="VMZ53_27820"
     CDS             2166..>3400
                     /locus_tag="VMZ53_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HUS32352.1"
                     /translation="MSETTSSNITPISIETEMRNSFMDYAMSVIVARALPDARDGLKP
                     VHRRILFAQRGLNNTYSRPYLKCARIVGDVIGKYHPHGDSAVYDALVRMAQDFSMRYM
                     LIDGQGNFGSIDGDPPAAMRYTECRMSKLSSELLADIDKETVDWQPNYDDKEEEPTVL
                     PTRVPNLLINGASGIAVGMATNIPPHNLGEIIDGTLALIENPKLTDDDLLTYVKGPDF
                     PTGGVIQGRNGIIGAFKNGRGTVVVRGVAAVEEIRKDRQAIIVSELPFMVNKARWIET
                     TADMVRDKKLEGISDIRDESDRTGIRVVFELKNDANDQVVLANLMKHTSLQSSFSVNM
                     LAIVDGRPVLLTLRRALQVFIEHRRDVVTRRTLYDLRQARERREIVEGLGLAVINIDR
                     VIEIIRGSKDTDIAKERLMA"
BASE COUNT          616 a         1113 c         1099 g          572 t
ORIGIN      
        1 atcggccagc ggttcccgca ggcgacgtgt gtcgtcgacg ctggcgcggt gcgcatcgag
       61 ctcgcgatcg acggcgtgac gtatcgcgca cgagctcaga tggagccgcg aggccgcgtc
      121 gtcgtccagc tgcccagcac ggatggcttc gagctggcgc tgtcgtggac ggatcgcgcg
      181 gagctcgtga ccggcacgcc gacgttcgac gacagctgcc tcgtcgagac gaatgatgtc
      241 tcgctcgcaa acatgtggct cgatgccgag gcgagagctg cgttgctcgc atcgcgatat
      301 gtctccggca caccgcaggc gctccgcacg acgatgcccc tcttgcgcga tggagcgtgg
      361 cagcacgagg tgcgcagcga cgaggtctgc gcgcagcgtg ccgatgccga ggacagtgcg
      421 gagcggatcg tcgacatgct cgctgcatcg ctcgcgctcg cgcgccggcc cgcgcggtgg
      481 gctcgctggt tcgcgccgct cgcgaagtcg ctcggcggtg aagcgtccgc gagggtcgag
      541 ctcggcggca ggcccgtgct gcgcgtgcgt cgcggcagca gcgacgtcag catccgcctg
      601 ctgcgccggc tcggccccgg tgacgtcggg cggctccgca ccgtgatcag cgcgcatcgc
      661 gtagcgtcga gcggcgaagt cctgacgctc atcgccgaag acctcccgcg ctcggcgtgg
      721 ccgcccgcga acgaccatac cgagagcacc cttcgcatcg atgcacgggc gcgcacgctg
      781 ctcgattccg cgcgtccctc gacgacgatc gtccgccgcc acgatgtcga gatcacgttc
      841 gatggcgccc ttgccgatcg tgatcgcctc ggcgctgccg tcgagctcgc cgcctactgg
      901 gcgacggatc acagccacgc cggtccgtat cgatgagata atgaagcggt gaggatggcc
      961 gttgtctggc tactggcgct gtgcgcgtgc gaatcgcgct atggcgacta cttcaacgtc
     1021 gacggcgaca gcaacggcat ccgcttcgat cacgtcgagc tgtacttcgg caagcacggc
     1081 atgagcgata gcaatatcgg caagcccagc ggctcgacga ccggcgacgt gttcatccgc
     1141 gacgcggatg cgagcgatgc gtgggacgtc gacaagaacg gcgacggcag cctcgcgagt
     1201 gagagcacgt actggcttcc gatcacggag cagaatcgcg agctcgcgta tgtcgctgcg
     1261 cttgcctacg acacgacgat gcagagcgac aagcccgtcg gcgtcggcga ggtgctcggc
     1321 ttcgcgctcg aagacggcat cgtcaacaag tacgacatcg agctgcagaa gttcgacttc
     1381 accgtcgata catggggcca gtcccctggc tgcttcgcgt ggacgcgtca gcgcgatggg
     1441 aagttctcga cgatcgccgt gctctctccc gacgacgcgg actgcgatgg cgtcgtcgcg
     1501 agcgcggatt gcaacgactt gtgccccgcg ggctcgacga agtgcaaccc cgatgaaacc
     1561 gtgtgcggtg gcgcaaattc gtgcggcctc ggttgcgcga tcaacggcct gtgccgcatc
     1621 gagacctgct tgcctacgat cgcgtgcacg aactgcggga tgtttgcggc ctcgctcgac
     1681 gaccgcatga aatgcgtctc gagtgatccg gcgcatcacc tctcggtgtt ggtcgacacg
     1741 ctcggtggca aaccgtgcgc gcgttcgttc gacgttccga actacggtcg tccgtgctcg
     1801 ggcgtgaccg aagtgtggca gcagaatttc ggtgatggct gggatctcac cgtctcgcca
     1861 tcggcgaatg atctcacgca ctgcatcgtc gagctcacga acacgagtac gagcgcgacg
     1921 tttgctggcg atcatcacgt gctcgttgcg ttcccgccgc gcgcgccgag cagctcgcaa
     1981 tggagcgtcc tgttgggcgt caaagacgta cctgcgacgg cgtgtcagat cgatggcgga
     2041 tacacgatcg acggcaacgt cggcgcgccc aacgactgcc tttaacgcgc cagaattgct
     2101 gagctaatag ctggctcggg cgcttgaggg ctcgcccctc caaaggctat acggaagtac
     2161 tccagatgtc cgagaccacg tcgtccaaca tcacgcccat ctcgatcgaa accgagatgc
     2221 gcaactcgtt catggattac gccatgagcg tcatcgtcgc gcgcgctctg cccgatgcgc
     2281 gagacggttt gaagcccgtg caccggcgca tcctgttcgc gcagcgaggc ctcaacaaca
     2341 cgtacagccg cccgtacctc aagtgcgcac gcatcgtcgg cgacgtgatc ggcaagtatc
     2401 acccgcacgg cgatagcgcg gtctacgacg cgctcgtccg catggcccaa gacttctcga
     2461 tgcgctacat gctcatcgac ggtcagggca acttcggttc gatcgacggc gatccaccgg
     2521 ccgcgatgcg ttacaccgag tgccggatga gcaagctctc ttcggagctg ctcgccgaca
     2581 tcgacaaaga aaccgtcgat tggcagccca actacgacga caaagaagag gagccgaccg
     2641 tcctcccgac gagggttccg aatctcctca tcaacggtgc atcgggaatc gccgtcggca
     2701 tggcgacgaa catcccgccg cacaacctcg gcgagatcat cgacggcacg ctcgcgctga
     2761 tcgagaaccc gaagctcacc gacgacgatc tgctcacgta cgtcaaaggc cccgatttcc
     2821 cgacgggcgg tgtgattcaa ggccgtaacg gcatcatcgg cgcgttcaag aacggtcgag
     2881 gcaccgtcgt ggtacgcggc gtcgcggccg tcgaggagat ccgcaaggac cgccaggcga
     2941 tcatcgtcag cgagctgccg ttcatggtga acaaggcgcg gtggatcgag accaccgccg
     3001 acatggttcg cgacaagaag ctcgagggca tctcggacat ccgcgacgaa tcggatcgca
     3061 ccggcattcg cgtcgtgttc gagctcaaga atgacgcgaa cgatcaggtg gtgctcgcca
     3121 acctgatgaa gcacacgtcg ctgcagagct cgttcagcgt gaacatgctc gcgatcgtcg
     3181 acggtcggcc ggtgctgctg acgctgcggc gtgcgctgca ggtgttcatc gagcaccgcc
     3241 gcgacgtcgt cacgcggcgc acgctgtacg acctccgcca ggcgcgcgag cggcgcgaga
     3301 tcgtcgaggg cctcggcctc gccgtcatca acatcgatcg cgtcatcgag atcatccgcg
     3361 ggtcgaagga caccgacatc gccaaggaac gcctgatggc
//
LOCUS       DATLBZ010000556         5875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10054171, whole genome shotgun sequence.
ACCESSION   DATLBZ010000556 DATLBZ010000000
VERSION     DATLBZ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10054171"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VMZ53_27825"
     CDS             complement(<1..549)
                     /locus_tag="VMZ53_27825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32353.1"
                     /translation="MVRAVAVLAILASGCRIGFDIFDGDDALDDAPRGDTTTPIDIIP
                     DGPAFDALISDGHLVGYWKLDDGFFVDSSTFGNNAGNCYASCPTLAPGVRGMAADFHN
                     MEGIEVGTVAPLSSLANNLTLAAWVNLRSITTYSAIISNDRDCSNCQTLSGFSLWASL
                     YDLAPTFLLWNQSMTSFGPNGPS"
     gene            738..1307
                     /locus_tag="VMZ53_27830"
     CDS             738..1307
                     /locus_tag="VMZ53_27830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32354.1"
                     /translation="MTKRILPFTILGSIFFALTAGHTRADEISAHETSAREQPHVAIT
                     VSPLHMFVPMAELTVEARVAPKVGVAAIGGAGTYRTMDTNDRISLIEAGASARYYAAG
                     SFRTGLQLGFEALYVHAFTNASNIDVKAAGLALSPFAGYKWTHSSGLTLEGELGVSYM
                     VARAEAATGQMAERKAYGPMLNLQVGYSF"
     gene            complement(1304..2782)
                     /locus_tag="VMZ53_27835"
     CDS             complement(1304..2782)
                     /locus_tag="VMZ53_27835"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HUS32355.1"
                     /translation="MSAEGTGLQTKVARGVAWAAGAQAIIALADLVSQMVVIALISQV
                     DLGLAGAAIPFYTMLDTAADLGVTSSLIQRDDHTPERVSTVFWFNMLVSTGLFLLLLV
                     VGPLYGYMQGHAIIGWLLVAYGGKLIYQNVYSIPFALLRKELRFADIAVARTIAHICE
                     SAARVVFALLGWGVWCFTLASLTRAFVFGVIMQIRHPFIPRFVFRPREVTQYVRFGLR
                     SAASQVLYQLYTNLDYPIVTYYFGAAANGLYFAAYAIVLEPVKTIANVVIDVAFPTFA
                     RLRNDRPALIDQFIKFTRLNLIAVLPFVVLVLLVIPEYLTLFTSHEKWSAHEIAVCSE
                     AARILCLVGMLRALGFLGPPLLDGIGHPERTLRYMVIAAIVVPGMFIISANAFGDEMG
                     FMSVAIGWSIGYPIAFVALSWLVVRGIDLPLGLYFRGAWGIVGTCAAGLCAGMAVSLA
                     LPHAGPAVRMLAIGGTAIGVIALLLAVWQKITPRSMLRAVRG"
     gene            2822..3481
                     /locus_tag="VMZ53_27840"
     CDS             2822..3481
                     /locus_tag="VMZ53_27840"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS32356.1"
                     /translation="MSERQVQVEPEHYDFERYDDAERWMSYWHQLRAVLSVRPKTVLE
                     IGPGSGVFRSYLRAKGIDVKTLDIDKSRDVDFVADITKLDETLPKGLVFDAVCAFQVL
                     EHLPWSELETCLGGIARRANPHVFISLPYRGLRVRWAFWWGDHHFTLGHKLMLPWRHK
                     PIPEHHWELGYPITAGKVTKVIKKHLDVVSKGFIRENPYHYMWQLKARPALPYTQSPQ
                     P"
     gene            complement(3435..4490)
                     /locus_tag="VMZ53_27845"
     CDS             complement(3435..4490)
                     /locus_tag="VMZ53_27845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32357.1"
                     /translation="MDLRGSPHFMIGIGNDNQGPYTNDVPIDLHYAYLVGYGDGTGWP
                     TWNTNGDYPLFFEQNATSHQVTAMFTYYQLALELENNSDTTLVDTTRMHQYLSDMRVL
                     FQKIAANGTPVAVNIEPDFFGYLMQRGSSAIPAKVHHSDVPECATLPETAAGLMQCVI
                     KLGRTIAPKARLGFHASMWGHWYDVLDPNADVEGAANAVGDFLMAISNDTDFVSVETL
                     DRDAGFWETSGGGSTCSTANGSRGPVYWDETNTSLPNFSQHLRWVKALTARMNRPAIE
                     WQTPLGVPSTTCGGSTDHWRDNRVHYFFGHVPDLVDAGIAGMTFGTGAGAQTNLATDG
                     GQFKAAATAYKAAPVAL"
     gene            complement(4592..5572)
                     /locus_tag="VMZ53_27850"
     CDS             complement(4592..5572)
                     /locus_tag="VMZ53_27850"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUS32358.1"
                     /translation="MESVAFLETTGDRHTQVVGGLTALERRIREVAKQGATRVVVAAA
                     PVAIARPLPVPVEYVAPGSAAPEGAKRERADVIAGVELVDEEARGRAEWALIRRMNKS
                     YEGPVDALINWRFSMRITRVLSHASLAVTPNHVTIAAILVGLFASWLAWQGGYVNVAI
                     AGFLLQVNSVLDSVDGELARLRYQFSKLGQWLDNLADDIVDNLFLAALGASLGGVWWW
                     LGLAAALARVAVALSTYLDVYRRTGTGDVFAFRLWFEKPDLTHDAAYKPTSVKTWLRS
                     LGRRDTFVFVWMIACLAGFPWWVIGHGLVIAAVNVALHVLHWLLSRPPAR"
     gene            complement(5624..>5875)
                     /locus_tag="VMZ53_27855"
     CDS             complement(5624..>5875)
                     /locus_tag="VMZ53_27855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32359.1"
                     /translation="DCANDPVDGANIVVKRNGSVVTGLTDFDLGAFSAMAAGVHVVFN
                     VAPGTVEVSAKVGTTTLQAHNVDVFMGGSTMTIVRPGYF"
BASE COUNT         1111 a         1896 c         1906 g          962 t
ORIGIN      
        1 ggatgggccg ttggggccga acgacgtcat gctctggttc cagagcagga acgtcggcgc
       61 caagtcgtac aggctggccc agagcgagaa cccgctcagc gtttggcagt tcgagcagtc
      121 gcgatcgttc gagatgatcg cggagtacgt cgtgatcgag cggagattga cccaggcggc
      181 gagcgtgagg ttgttggcga gcgatgagag cggcgcgacg gtgccgactt cgatgccctc
      241 catgttgtgg aagtcggcgg ccatgccgcg gacgccgggc gcgagcgtcg ggcagctcgc
      301 gtagcagttc ccggcgttgt tgccgaacgt cgagctgtcg acgaagaagc cgtcgtcgag
      361 cttccagtag ccgacgagat ggccgtcgct gatgagcgcg tcgaacgccg ggccatcggg
      421 gatgatgtcg ataggcgtcg tggtgtcgcc gcgtggcgcg tcatcgagcg cgtcgtcgcc
      481 atcgaagatg tcgaagccga tgcggcatcc gctcgcgagg atcgcgagga ccgctacagc
      541 gcgcaccatg gcccattgta gccgcggcga tttcggacat tgctgtcagc gcgtgaagat
      601 tcgttcacgg cggcgtcggg tgtgtgaggt cacaccgttg cggcccgcga gcttacggcg
      661 cgtccggtaa accacgacgt gcgtgtcgca ggtgctgcca tgcgcccggt ggctcggacc
      721 ttgatgaagg cgggcgcatg acaaagcgta tcctcccgtt cacgatcctc ggttcaatct
      781 tctttgcgct cacggccggc cacacgcgtg ctgacgagat ttctgcgcac gagaccagcg
      841 cgcgcgagca gccacatgtc gcgatcaccg tctcgccgct gcacatgttc gtgccgatgg
      901 ccgagctgac ggtcgaggct cgcgtggcgc ctaaggtcgg cgttgctgcg atcggcggtg
      961 ccgggacgta tcgcacgatg gacacgaacg atcgcatctc gctcatcgag gccggcgcga
     1021 gcgcgcgtta ctacgcagct ggctcgttcc ggacgggcct gcagctcgga ttcgaggcgc
     1081 tgtacgttca cgcgtttacg aatgcgagca acatcgacgt gaaagctgcg ggtctcgcgc
     1141 tctcgccgtt tgctggctac aagtggacgc attcgtcggg cttgacgctc gaaggcgagc
     1201 tcggcgtttc gtacatggtc gcgcgcgcgg aagcggccac cggtcagatg gcggagcgca
     1261 aggcgtacgg cccgatgctg aacctacagg ttggctacag cttctagccg cggacggctc
     1321 tgagcatcga gcgcggcgtg atcttctgcc acacggcgag caagagcgcg atcacgccga
     1381 tcgcggtgcc accgatcgcg agcatgcgaa cagcggggcc cgcgtgcggc agcgcgagcg
     1441 agacggccat gccggcacac aagcctgcgg cacacgttcc gacgatgccc cacgcgcccc
     1501 tgaagtagag gccgagcggg aggtcgatgc cgcgcacgac gagccacgat agtgcgacga
     1561 acgcgatcgg atagccgatg gaccagccga tcgcgacgga catgaagccc atctcgtcgc
     1621 cgaatgcgtt cgccgaaatg atgaacatgc ccggcacgac gatcgctgcg atcaccatgt
     1681 agcgcagcgt gcgctcgggg tggccgatac cgtcgaggag cggcggtccg aggaacccga
     1741 gcgcgcgcag cattccgacg agacacagaa tgcgcgccgc ctcggagcag accgcgatct
     1801 cgtgcgcgct ccacttctca tgcgacgtga acagcgtcag gtactccggg atcacgagca
     1861 ggacgagcac cacgaacggc agcaccgcga tcaagttcag gcgcgtgaac ttgatgaact
     1921 gatcgatgag cgcgggccga tcattgcgca ggcgcgcgaa cgtcgggaac gcgacatcga
     1981 tgacgacatt cgcgatcgtc ttcaccggtt cgagcacgat cgcgtacgca gcgaagtaga
     2041 ggccgttggc cgccgcgccg aagtagtacg tgacgatcgg ataatcgagg ttcgtgtaca
     2101 gctggtagag gacttggctc gccgcgctgc gcaggccgaa gcgcacgtac tgcgtgacct
     2161 cgcgtgggcg gaacacgaag cgagggatga acggatggcg gatctgcatg atcacgccga
     2221 acacgaacgc gcgtgtcagc gatgcgagcg tgaagcacca aacgccccag ccgagcaatg
     2281 cgaacacgac gcgtgccgcc gactcgcaga tatgcgcgat cgtgcgcgcg acggcgatgt
     2341 cggcaaaccg cagctccttg cgcaggagtg caaacggaat cgagtagacg ttttgataga
     2401 ttagcttgcc gccgtacgcg acgagcagcc agccgatgat cgcgtggccc tgcatgtagc
     2461 cgtacagcgg cccgacgacg agcagcagca agaacaagcc cgtcgagacg agcatgttga
     2521 accagaagac cgtcgagacg cgctcgggcg tgtggtcatc gcgttgaatg agcgacgacg
     2581 tgacgccgag gtcggccgcg gtatcgagca tcgtgtagaa cggaatcgcc gcgcccgcga
     2641 gacccaagtc cacttgcgag atgagggcaa tgaccaccat ctgcgagaca agatcggcaa
     2701 gcgcgatgat cgcttgcgcg cccgcggccc acgcgacgcc acgcgctacc ttggtttgca
     2761 ggcctgtgcc ttcggccgac acaacgagcg cgagaatagc gtgctagcgt tcgtggcgta
     2821 cgtgagtgaa cggcaagttc aagtcgagcc cgagcattac gatttcgagc gctacgacga
     2881 tgcggaacgc tggatgtcct attggcacca gctccgcgct gtcctctccg tgagaccgaa
     2941 gaccgtcctc gagattgggc ccggctccgg cgtcttccgc agttacctgc gcgccaaagg
     3001 catcgacgtc aagacgctcg atatcgacaa gtcgcgcgac gtcgacttcg tcgccgacat
     3061 cacgaagctc gacgagacgc tgcccaaggg cctcgtcttc gacgcggtgt gcgcattcca
     3121 agtcctcgag catctgccgt ggagcgagct cgagacgtgc ctcggcggca tcgcgcgccg
     3181 cgccaacccg cacgtgttca tctcgctgcc gtatcgcggt ctgcgcgttc gctgggcgtt
     3241 ttggtggggc gaccaccact tcacgctcgg ccacaagctc atgctgccgt ggcggcacaa
     3301 gccgattccc gagcatcact gggagctggg ctacccgatc actgcaggca aagtcacgaa
     3361 ggtgatcaag aagcatctcg acgtcgtgtc gaaaggcttc atccgcgaga acccgtatca
     3421 ctatatgtgg cagctcaaag cgcgaccggc gctgccttat acgcagtcgc cgcagccttg
     3481 aactgaccgc cgtcggtcgc gaggtttgtc tgcgcgccag cgccggtgcc gaacgtcatg
     3541 ccggcgatgc ccgcgtcgac gagatcgggc acatgcccga agaagtagtg cacgcggttg
     3601 tcgcgccagt ggtcggtcga tccgccgcag gttgtcgacg gcacgccgag cggcgtctgc
     3661 cattcgatcg ccggacgatt catccgcgcg gtcagcgcct tcacccaacg caggtgctgg
     3721 ctgaagttcg gcagcgacgt gttcgtctcg tcccagtaga cggggccgcg agagccgttc
     3781 gccgtcgagc acgtcgagcc accgccgctc gtctcccaaa agcccgcgtc gcgatcgagc
     3841 gtctcgacgc tcacgaaatc cgtgtcgttc gagatcgcca tcaagaaatc gccgaccgca
     3901 ttcgccgcgc cctcgacgtc cgcattcgga tcgagcacgt cgtaccagtg gccccacatc
     3961 gatgcgtgaa agccaagccg agctttcggc gcgatcgttc gaccgagctt gatcacgcat
     4021 tgcatgagac cggccgccgt ctcgggcaag gtcgcgcact cgggcacgtc gctgtggtgc
     4081 accttcgccg gaatcgccga cgagcctcgc tgcatcaagt agccgaagaa atcgggctcg
     4141 atgttgacgg cgacgggcgt gccattcgcc gcgatcttct ggaacaagac gcgcatgtcg
     4201 ctcaagtact gatgcatgcg cgtcgtgtcg acgagcgtcg tgtcgctgtt gttctcgagc
     4261 tcgagcgcga gctggtagta cgtgaacatc gcggtgacct gatgcgacgt cgcgttctgc
     4321 tcgaagaaga gcggatagtc gccgttcgtg ttccacgtcg gccatcccgt gccgtcgccg
     4381 taccctacga gatacgcgta atgcagatcg atcggcacgt cgttcgtgta cggcccctgg
     4441 ttgtcgttgc cgataccgat catgaagtgc ggtgagccgc gcagatccat cgcgagctgg
     4501 cgctgccggt cgcgcagcgt gcttccgccg ccattcgagc cgtctccgtt ggaatcggga
     4561 tcgccgttct ccgaggacga accgcacgcg actagcgcgc tggcggccgc gacaacagcc
     4621 agtgcagcac gtgcaacgcg acgttgaccg cggcgatcac gagcccgtgt ccgatcaccc
     4681 accacggaaa tcccgccaga cacgcgatca tccatacgaa cacgaatgta tctcgcctac
     4741 cgagcgagcg cagccacgtc ttcaccgacg tcggcttgta ggccgcatcg tgcgtcaaat
     4801 caggcttctc gaaccacagc cgaaacgcaa agacgtcgcc ggtcccagta cggcgataga
     4861 cgtcgaggta ggtggacagc gcgaccgcaa cgcgcgcgag tgccgcagcc aaaccaagcc
     4921 accaccacac gccgccgagg ctcgcgccga gcgcagcgag aaacagattg tcgacaatgt
     4981 cgtcggcgag gttgtcgagc cactgcccga gcttgctgaa ctgatagcgc agccgcgcga
     5041 gctcaccgtc gacggaatcg aggacggagt tcacctgcaa caggaagccc gcgatcgcga
     5101 cgttcacata gccgccttgc cacgcgagcc agctcgcaaa cagcccgacg aggatcgcgg
     5161 cgatcgtgac gtgattcggc gtgaccgcga gcgacgcatg cgagagcacg cgcgtgatcc
     5221 gcatcgagaa ccgccaattg atcagcgcgt cgacgggtcc ctcatacgac ttgttcatgc
     5281 gccggatgag cgcccactcc gccctgccgc gcgcctcctc gtcgacgagc tcgacgcccg
     5341 cgatcacatc cgcgcgctcg cgcttcgcac cctccggcgc cgcactccca ggcgcgacgt
     5401 actcgacagg cacgggcagc ggccgcgcaa tggcgacggg tgcagccgca accacgacgc
     5461 gggtggcgcc ttgcttcgcg acttcgcgga tgcgccgctc gagagcggtc agcccgccga
     5521 cgacctgggt gtgcctgtca cccgtggtct cgaggaacgc aacgctctcc actccgtgtc
     5581 agataccacg agcgtcgcgg aaggatcccg caacgtctgg caatcagaag tagccgggac
     5641 ggacgatcgt catcgtcgag ccgcccatga agacgtcgac gttgtgcgct tgcagcgtcg
     5701 tggtgccgac cttcgcgctc acctcgaccg tgcccggcgc aacgttgaat acgacgtgaa
     5761 cgccagcggc catcgcgctg aacgcgccga gatcaaaatc cgtcaggccg gtaacgacgg
     5821 agccgttccg cttcacgacg atgttcgcgc catcgaccgg atcgttcgcg cagtc
//
LOCUS       DATLBZ010000557         4772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2970048, whole genome shotgun sequence.
ACCESSION   DATLBZ010000557 DATLBZ010000000
VERSION     DATLBZ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2970048"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..537)
                     /gene="ribH"
                     /locus_tag="VMZ53_27860"
     CDS             complement(55..537)
                     /gene="ribH"
                     /locus_tag="VMZ53_27860"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HUS32360.1"
                     /translation="MPTTIEGSLSAAGLRFAIVASRFNSLIVEPLVQGAVDAIVRSGG
                     NADDITIVRCPGAWELPQVVRKVVERGGVDAVVALGAVIRGSTPHFDYVAGEASRGIA
                     SAASADIPISFGVLTTDTIEQALERAGTKAGNKGFDAAMAAIELAQLFRALSGRKGKK
                     "
     gene            complement(553..1749)
                     /gene="ribB"
                     /locus_tag="VMZ53_27865"
     CDS             complement(553..1749)
                     /gene="ribB"
                     /locus_tag="VMZ53_27865"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HUS32361.1"
                     /translation="MNAIERVQRALEEIRAGKMVILVDDEDRENEGDLVFAAELVTPE
                     HINFMATHARGLICLAMDDAMIDKLELAPMVRDNQASLGTAFTVSIEARHGVSTGISA
                     RDRATTIRAAIADDAGPHKIVSPGHIFPLRARKGGVLVRTGQTEGSVDLARLAGLKSA
                     GVICEIMREDGEMARRPELEEFAKKHGLLLLSVADMIAYRLQRERIVRCQQSGMLRPG
                     ALGPGEPFAAHYYETEVEDTEYLALVRGDVAGVSAAGGTVLVRVAAIDPLVDPFALRP
                     DLVSALKAIDDAGCGVFLYVVNRNRMKLKSSFERLVMRPKVAELWQDREATKPGVGSS
                     AQTSEALRDFGLGAQVLADLGCHKIRLMMWSDRKIPGLEGFGIDVVERVPIPPPPAAR
                     AAKSQS"
     gene            complement(1746..2342)
                     /locus_tag="VMZ53_27870"
     CDS             complement(1746..2342)
                     /locus_tag="VMZ53_27870"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HUS32362.1"
                     /translation="MFTGLVEDVGTVLRADRRSNALVLAIKPARIPTSELAIGESVCH
                     DGACLTVTETARDSFTVLAGLETLERTTLGSLRVGKRVNLERSMKVGDRLGGHWVTGH
                     IDGTGELAARRDLGSNLVLTVRTIPALLKYIVEKGSIAIAGISLTVNAVDAETFSVAI
                     IPHTRDMTTLGDLAIGDRVNLETDILAKHVEKLLGGRS"
     gene            2539..3921
                     /locus_tag="VMZ53_27875"
     CDS             2539..3921
                     /locus_tag="VMZ53_27875"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HUS32363.1"
                     /translation="MNNATQTKTLLAGLFIALSTSTAAAQSASETQLTDKVEAPAKAE
                     SDSIGTVKTKGPRHMRMLAEGAAIQAIGTAWYWRNTGSGWGESNRVDWQLGFKGSALV
                     AKLDGSGWRNDGNPYSINALCHPMSGSLTYYLARGNGYSPLGAFVVSSLVSVSWELFT
                     EYAEYGAPNDLLATSPAGFSIGESVYQIRHNWKRTAKQLSIGAGSNDGDGFISLGGRL
                     SLDTLPTDEGSGVVVGGKKAAVGIEVPFDGAIRAVEAGAKSSVVGYYRNGKANRLFAG
                     ASSEFYFRNQSQRDSRDWDYMTTVSMGPTVDAKLQLSDATIDIGTDVYAEFGLLKSEA
                     FESWRADHPMALVRNSMQDKADPYYYAYGVAVTPRINVAYRGLNVGGKVAAHAFKSMN
                     GIDRDQEMMTADPSMRDTDVTAEAWLAYSHKSVTLSVDGRAHNRTGKIDSARGSQSDT
                     TTMFSVAYNR"
     gene            complement(3918..>4772)
                     /locus_tag="VMZ53_27880"
     CDS             complement(3918..>4772)
                     /locus_tag="VMZ53_27880"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HUS32364.1"
                     /translation="GGRDPIRIILDSTLRIPTSARVLRGGARTIIATTSRASEAKEKK
                     LVKMGVEVWRIEPPSALGGEDRKDEKAPSSTSQVASIASGTSKVANSASGTSKVANSA
                     SGTSNVATGTSKVASGTSNVAGIVGGTSKVAHVARGSSDVASGASRAAGGTSKVALGA
                     AKVVLGASKVARAASKAASAVKGRVAGRGKGGGVSLETLGKWLAQEGITSVLVEGGGE
                     VHASFLAADLADELCVYVAPKVVGGPAPSWVGGAGIGDIDLAHQFSFVDAVVIDGDMR
                     LRAVRRRR"
BASE COUNT          801 a         1674 c         1522 g          775 t
ORIGIN      
        1 gcgcgtagag gagctggagc gcgtaagcgc gtcctgtgcg gcgtacgcct gcggtcactt
       61 cttgcccttc cgaccgctga gcgcgcggaa cagctgcgcg agctcgatcg ctgccatcgc
      121 cgcgtcgaag cccttgttgc ccgccttggt gccggcgcgc tcgagtgctt gctcgatcgt
      181 atcggtcgtc agcacgccaa agctgatcgg gatgtctgcg ctcgccgcgc tcgcgatacc
      241 acgggaggct tcaccggcga cgtaatcgaa gtgcggcgtc gagccgcgaa tgaccgcgcc
      301 gagcgccacg actgcatcga cgccaccacg ctcgacgacc ttgcgcacga cctgcggcag
      361 ctcccatgca ccggggcagc gaacgatcgt gatgtcgtct gcgttaccgc ccgatcgcac
      421 gatcgcatcg accgcgccct gcacgagcgg ctccacgatc aggctgttga agcggctcgc
      481 gacaatcgcg aaacgcagcc cggcggcaga cagagagcct tcaatggtgg tcggcatggg
      541 tgcccagata tatcagcttt gcgactttgc agcgcgagcc gcgggaggcg gaggaatggg
      601 cacgcgttcg accacgtcga tgccaaagcc ttcgaggccc ggaatcttgc gatcgctcca
      661 catcatcaag cggatcttgt gacagcccag atcagcgaga acctgcgcac cgagcccgaa
      721 gtcgcgcagc gcctcgctcg tttgtgcgct cgagccgacg cctggcttcg tcgcttcgcg
      781 gtcctgccag agctcggcaa ctttgggccg catgaccagc cgctcgaacg agctcttgag
      841 cttcatgcgg ttgcgattga cgacgtagag aaacacgccg cagcccgcat catcgatggc
      901 cttgagcgcg ctgacgagat ccggacgcag cgcgaatgga tcgacgagtg gatcgatcgc
      961 agcgacgcgc acgaggacgg tgccgcccgc cgctgacaca ccagccacgt cgccgcggac
     1021 gagcgcgagg tactcggtgt cttcgacctc cgtctcgtag tagtgcgccg cgaacggctc
     1081 gccgggtccg agcgcgccgg gccgaagcat gccgctctgc tggcagcgaa cgatgcgctc
     1141 gcgctggagc cgatacgcga tcatgtcggc gacgctgagc aggagaaggc cgtgcttctt
     1201 cgcgaactct tcgagctcgg ggcgacgcgc catctcgccg tcctcgcgca tgatctcgca
     1261 gatgacgccg gcagacttca ggccggcgag ccgcgcgagg tcgacggaac cttccgtctg
     1321 gccggtgcgc acgagcacgc cgcctttgcg cgcgcgcagc ggaaagatgt ggcccggcga
     1381 gacgatcttg tgcgggcccg cgtcgtcggc gatggcagcg cgaatcgtcg tcgcgcgatc
     1441 gcgcgcggag atgcccgtcg acacgccgtg gcgtgcttcg atcgaaaccg tgaaggcggt
     1501 gccgagcgac gcttggttgt cgcgcaccat cggtgcgagc tcgagcttgt cgatcatcgc
     1561 gtcgtccatc gcgaggcaga tgaggccgcg cgcgtgcgtc gccatgaagt tgatgtgctc
     1621 gggcgtgacg agctcggccg cgaacacgag gtcgccctcg ttctcgcgat cttcgtcgtc
     1681 gacgaggatc accatcttgc cggcgcgaat ctcttccagc gcgcgctgga cgcgttcgat
     1741 cgcattcatg agcggcctcc taagagcttc tcgacatgct tcgccaaaat atccgtctcg
     1801 aggttcacgc gatcgccgat cgcgagatcg ccgagcgtgg tcatgtcgcg cgtgtgcggg
     1861 atgatcgcga ccgagaacgt ctcggcgtcg accgcattca cggtgagcga gatgccggcg
     1921 atcgcgatcg agcccttctc gacgatgtac ttcagcagcg caggaatcgt gcggacggtg
     1981 agcacgaggt tcgagccgag gtcgcgacgc gcggcgagct cacccgtgcc gtcgatgtga
     2041 cccgtcaccc agtgaccgcc gagccggtcg ccgactttca tcgatcgctc gaggttcacg
     2101 cgcttaccga cgcgcagcga gccgagtgtc gtgcgctcga gcgtctcgag acctgcgagc
     2161 accgtgaacg aatcgcgcgc ggtctcggtg accgtcaaac acgcgccgtc gtgacaaacg
     2221 ctctcgccga tcgcgagctc tgacgtcggg atccgcgcgg gcttgatcgc gaggacgagc
     2281 gcattggagc gccgatcagc acgcaaaacg gtgccgacgt cttcgaccag acccgtaaac
     2341 atggctgcgg tctaccacgg ctaggaagca cagcggaacc ggccagttgc cgcggtttct
     2401 cgtgcgccgt tcacgcgaat ttcccgcggt tcgccacgcg aatctcaggc accgaaacgc
     2461 gtctctcccg cgccgtgtca ctcgcggatc acccagtggt tctgggggct tcacgcccga
     2521 tcgccgcacc ttgggttcat gaacaacgcg acgcaaacca agaccctcct cgccggcctc
     2581 ttcatcgctc tgtcgacctc gaccgccgcc gcccagtcgg cttccgagac ccagctgacg
     2641 gacaaggtcg aagccccggc gaaggccgaa tcggactcga tcggcacggt caagacgaag
     2701 ggcccgcgcc acatgcgcat gctggccgag ggcgccgcga ttcaagcgat cggtacggcg
     2761 tggtactggc gcaacacggg ctcgggctgg ggcgagtcga accgcgtcga ctggcagctc
     2821 ggcttcaagg gcagcgccct cgtcgcgaag ctcgatggct cgggctggcg taacgacggc
     2881 aacccgtact cgatcaacgc gctctgccac ccgatgtcgg gctcgctgac ctactacctc
     2941 gcgcgcggca acggctactc gccgctcggc gcattcgtgg tttcgtcgct cgtctcggtg
     3001 tcgtgggagc tcttcacgga gtacgccgag tacggcgcgc cgaacgacct gctcgcgacg
     3061 tcgcccgccg gtttctcgat cggcgagtcg gtctaccaga tccgccacaa ctggaagcgc
     3121 accgcgaagc agctctcgat cggtgcaggc tcgaacgatg gtgacggctt catctcgctc
     3181 ggcggtcgcc tctcgctcga cacgctgccc acggacgaag gctcgggcgt ggtcgtcggc
     3241 ggcaagaagg cggcggtcgg catcgaggtc ccgttcgacg gcgccatccg tgcggtcgag
     3301 gcaggcgcga agtcgagcgt cgtcggctac taccgcaacg gcaaggcgaa ccgcctgttc
     3361 gccggcgcga gcagcgagtt ctacttccgc aaccagagcc agcgcgactc gcgcgactgg
     3421 gactacatga cgacggtctc gatgggcccg accgtcgatg cgaagctcca gctctccgac
     3481 gcgacgatcg acatcggcac ggacgtctac gcggagttcg gcctgctcaa gtcggaagcg
     3541 ttcgagtcgt ggcgcgctga ccacccgatg gcgctcgtcc gcaactcgat gcaggacaag
     3601 gccgacccgt actactacgc gtacggcgtg gcggtcaccc cgcgcatcaa cgtcgcgtac
     3661 cgcggcctca acgtcggcgg caaagtcgca gcgcacgcgt tcaagtcgat gaacggcatc
     3721 gaccgcgacc aggagatgat gacggccgac ccgtcgatgc gcgacacgga cgtgaccgcc
     3781 gaagcgtggc tcgcgtactc gcacaagtcg gtgacgctct cggtcgacgg ccgcgcgcac
     3841 aaccgcaccg gcaagatcga ctcggcgcgc ggctcgcagt cggacacgac gacgatgttc
     3901 tcggtcgcct acaaccgcta acgccgacgc cgcaccgcac gcagccgcat atcaccgtcg
     3961 atcaccaccg catccacgaa ggagaactga tgagccaaat caatgtcacc aatgcccgca
     4021 ccgcccaccc agctcggcgc cggcccgcca acgaccttcg gcgcaacgta cacacaaagc
     4081 tcgtccgcca agtccgccgc caagaacgac gcatgcacct caccgccgcc ctcgacgagc
     4141 acagacgtga tgccctcctg cgcgagccac ttgccgagag tctcgagcga cacgccgccg
     4201 cccttccctc gccccgcgac gcgccccttc acagcagatg cggccttcga ggcagcgcgc
     4261 gcgaccttcg aggcgccgag caccaccttc gctgcgccga gcgcgacctt cgacgtgcct
     4321 ccggcggccc tcgatgcgcc gctcgccacg tccgatgacc cgcgcgcgac gtgcgcgacc
     4381 ttcgatgtgc cgccgacgat gcccgcgacg ttcgacgtcc cgctcgccac cttcgatgtc
     4441 ccggtcgcga cgttcgatgt gccgctcgcg ctgttcgcga ccttcgatgt gccgctcgcg
     4501 ctgttcgcaa ccttcgacgt gccactcgcg atgctcgcga cttgcgatgt gctgctcggt
     4561 gccttttcgt ctttgcggtc ctctccgccc agcgcgctag gcggctcgat gcgccacacc
     4621 tcgacgccca tcttcaccag cttcttctct ttcgcctcgg aggcgcgtga cgtcgtcgcg
     4681 atgatggttc gcgcgccgcc gcgcagcacg cgcgctgagg tcggaatgcg cagcgtgctg
     4741 tcgagaatga tgcggatcgg gtcgcggcca cc
//
LOCUS       DATLBZ010000558         2745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2976776, whole genome shotgun sequence.
ACCESSION   DATLBZ010000558 DATLBZ010000000
VERSION     DATLBZ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2976776"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VMZ53_27885"
     CDS             complement(<1..282)
                     /locus_tag="VMZ53_27885"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HUS32365.1"
                     /translation="MSTRGDEGEGGNERALIRGLGPWASASIVVGTIIGTGIFLKTAV
                     MAQVGGSAVWVLAAWAVAAALSLFGALTYAELGAMFPRAGGEYVFLREGY"
     gene            complement(287..2041)
                     /locus_tag="VMZ53_27890"
     CDS             complement(287..2041)
                     /locus_tag="VMZ53_27890"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HUS32366.1"
                     /translation="MTKVVDQKSPLAAEATAGVSDATLRGVVADHWEFAMRWAPTWAT
                     TLGDHRFDDKLAPRDSASIQKMDEERDALLARMMAIDPTKLEEVDRVTYEELRGRLEA
                     EHALAICDNHEWVIDSSGGSLLGELSYLVESHTVKKPQDAENLIARLSQGGKLIDDTI
                     ANLKIGMSSGRVSSAEKVRRVIEQLDTELDKGVDGWAMATPKWTSSPEPDPWPEGVRD
                     RLNAKLREVVSQQIVPATVRFRDFLRDELLPKARTEKEGLSALPDGDACYRASILYHV
                     GLPKTPEELHALGLAEIARTDKELAALGAKVLGTKDLPSTIAKLRDDKALYFSSREEL
                     LAAAQRSLDKAKAAIPRYFALLPKADCVMREIPDYEAPYSTIAYYRQPHYDGSKPGEY
                     FVNTYKPETRPKFELEALTWHESIPGHHLQIAIAQELGQLPMFRKLEGSTAFVEGWAL
                     YTERLADEMGLYSSDTDRLGKVSYDAWRASRLVVDTGIHAMGWTRAQAEAFMHEHTAL
                     TAINISNEVDRYIGWPGQALAYKVGQIEILRLRADAEQKLGKRFELKSFHSVVLGAGA
                     VTLPTLGRRVNAWVDNAR"
     gene            2140..>2745
                     /locus_tag="VMZ53_27895"
     CDS             2140..>2745
                     /locus_tag="VMZ53_27895"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease"
                     /protein_id="HUS32367.1"
                     /translation="MQVHLFDGTYELFRAWFGAPPAQHEGREVGATRSVMRSLAMLIG
                     RGTVTHAGVAFDHVIESFRNEMFDGYKTGEGLDERLVSQFELVERATAAMGLVVWPMV
                     EFEADDAIATAAAALARDPRVEQVRIVSPDKDMCQCVEGGRVVCWERWKDIVLDEPAV
                     VAKHGVLPESIADLLALVGDTADGIPGLEGWGKSSAAKVLTV"
BASE COUNT          429 a          900 c          923 g          493 t
ORIGIN      
        1 gtacccctcg cgcaagaaca cgtactcgcc gccagcgcgc ggaaacatcg cgccgagctc
       61 cgcatacgtc agcgcgccga acagcgagag cgcggccgcg accgcccacg cggcgagcac
      121 ccacaccgca gagccgccga cctgcgccat gactgcggtc ttgagaaaga tgccggtgcc
      181 gatgatcgtg ccgacgacga tcgacgccga agcccacggc ccgaggccac gaatcagcgc
      241 gcgctcgttg ccgccctcgc cctcgtcgcc tcgcgtgctc atgcgatcag cgcgcgttgt
      301 cgacccacgc gttcacgcgg cgaccgagcg tcggcagcgt cactgcaccc gcgccgagca
      361 cgaccgagtg gaacgacttc agctcgaagc gcttgccgag cttctgctcg gcgtcggcgc
      421 gcagccgcag gatctcgatt tggccgacct tgtacgcgag cgcttggccc ggccagccga
      481 tgtagcgatc gacctcgttc gagatgttga tcgccgtcag cgcggtgtgc tcgtgcatga
      541 acgcctcggc ttgcgcgcgc gtccagccca tcgcgtggat gcccgtatcg acgacgaggc
      601 gcgatgcacg ccacgcgtcg tagctgactt tgcccaggcg atcggtgtcg ctcgaataga
      661 ggcccatctc gtccgcgagc cgctcggtgt agagcgccca gccttcgacg aatgccgtcg
      721 agccttcgag cttgcggaac atcggcagct ggccgagctc ttgcgcgatc gcgatctgga
      781 gatggtggcc tgggatcgac tcgtgccatg tcagcgcttc gagctcgaac ttcgggcgcg
      841 tctccggctt gtacgtgttg acgaagtact cgcccggctt gctgccgtcg tagtgaggct
      901 ggcggtagta cgcgatcgtc gagtacggcg cttcgtagtc gggaatctcg cgcatcacgc
      961 agtccgcttt cggcagcagc gcgaagtagc gcgggatcgc ggccttggct ttatcgagcg
     1021 agcgctgcgc agcggcgagc aactcttcgc gcgacgagaa gtagagcgct ttgtcgtcgc
     1081 gcagcttcgc gatcgtcgac gggagatcct tcgtgccgag gacttttgcg ccgagcgcgg
     1141 cgagctcttt gtcggtgcgc gcaatctcgg cgaggccgag tgcgtgcagc tcctcgggcg
     1201 tcttcggcag ccccacgtga tagaggatcg acgcgcgata gcaggcgtcg ccgtcgggca
     1261 gcgcggagag gccctctttc tcggtgcgcg ctttggggag cagctcgtcg cggaggaagt
     1321 cgcggaagcg gacggtcgcg ggaacgatct gctgcgagac gacctcgcgc agcttcgcgt
     1381 tcaagcgatc gcgcacgcct tcgggccacg ggtcgggctc cggtgacgat gtccatttcg
     1441 gtgtcgccat cgcccacccg tcgacgcctt tgtcgagctc ggtgtcgagc tgctcgatca
     1501 cgcgacgaac tttttcggcg ctcgagacgc ggccgctcga cattccgatc ttgagattcg
     1561 cgatcgtgtc gtcgatgagc ttgccgcctt gcgagaggcg cgcgatcaga ttctcggcgt
     1621 cctgcggctt cttcaccgtg tgcgactcga cgagatagct gagctcgccg aggaggctcc
     1681 cgccgctcga gtcgatcacc cactcgtggt tgtcgcagat cgcgagtgcg tgctccgctt
     1741 cgaggcggcc gcgcagctct tcatacgtca cgcggtcgac ctcttcgagc ttcgttggat
     1801 cgatcgccat catccgtgcg agcagcgcgt cgcgctcttc atccatcttc tggatcgatg
     1861 cggagtcgcg cggcgcgagc ttgtcgtcga atcgatgatc gccgagcgtc gtcgcccagg
     1921 tcggcgccca tcgcatcgcg aattcccagt gatcagcgac gacgccgcgc aacgttgcgt
     1981 ccgacacgcc cgcagttgct tcggcggcga gcggggactt ctgatcgacg accttggtca
     2041 tagacggccc cttcggcgta cacgcagctg tcgcgatcag cagaagagca gcgtgcgaca
     2101 gcctcgccat gcccctcgct tgtactacgc ttcggcgcgg tgcaggtcca tctgttcgac
     2161 ggtacgtacg agctgttccg ggcgtggttt ggcgcccctc ccgcgcagca cgaaggccgc
     2221 gaggtcggcg cgacgcgcag cgtgatgcga agcctcgcga tgctgatcgg ccgcggcacc
     2281 gtcacgcatg cgggtgtcgc gttcgatcac gtgatcgagt cgttccgcaa cgagatgttc
     2341 gacggctaca agaccggcga aggcctcgac gagcgactcg tgtcgcagtt cgagctcgtc
     2401 gagagagcga ccgccgcgat ggggctcgtc gtgtggccga tggtggagtt cgaggccgat
     2461 gatgcgatcg cgacagcagc ggcggcactc gcccgcgatc cacgcgtcga gcaagtgcgc
     2521 atcgtgtccc ccgataaaga catgtgccag tgcgtcgagg gcggaagggt cgtgtgctgg
     2581 gagcgttgga aagacatcgt gctggacgaa cccgcagtcg tcgcgaagca cggcgtgctg
     2641 ccggaatcga tcgccgacct gctcgcgctc gtcggagata ccgcggacgg catccctggc
     2701 ctcgagggtt ggggcaagag cagcgccgcg aaagtgctca cggtc
//
LOCUS       DATLBZ010000559         7042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_9682430, whole genome shotgun sequence.
ACCESSION   DATLBZ010000559 DATLBZ010000000
VERSION     DATLBZ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9682430"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VMZ53_27900"
     CDS             <1..371
                     /locus_tag="VMZ53_27900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32368.1"
                     /translation="LLAIAATVLLRRGAGSWRAAGRRLVRPPVEAIFFVPIAAVLIAV
                     ASTGNPLVGRAVWGIAIAGVVTSWITGALLGGTRVSLKRAVAHALCAMLAVGAATYVA
                     VDRGHLVDFLVETWREGPDR"
     gene            complement(372..1766)
                     /locus_tag="VMZ53_27905"
     CDS             complement(372..1766)
                     /locus_tag="VMZ53_27905"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUS32369.1"
                     /translation="MRACWGVLLLVAGCISAAADPGTARRANESRAREIYAELISIDT
                     SDTGDTAKAAHAMERRLVAAGLPAEDVKVFETGTKRGNLVARYRGTGRKRPIIVMAHI
                     DVVPAKRSDWSTDPFELVEKDGYFYGRGTTDDKFMAAAFVSALIRYKAESYKPDRDII
                     LVLATDEEEGDTGKGLSWLLANQRPLLDAELALNEGGWVGMRDGKPIFNSIQVAERNY
                     QSYFIEVHNRGGHAGRPRKDNAIYDLAAALARLGALQFPIEVSELTRQYIETIARHES
                     PEIAATLRGALEPSPSAAVLERLRDLPEYNGQLRTTCAATQVEAGENEGALPRTARAT
                     LTCLILPTHTTEDVQKVLEQTIAGTGATVTPVRKEPSGGPSKLPPELLATIATVSKKH
                     WPDAEVIPSMSLGSSDGVFLRGAGIPTFGNSGLAVDLLDTRLHGKDERVAVSAFFEGQ
                     QYLYELVKAIAGGK"
     gene            complement(1789..2259)
                     /gene="sixA"
                     /locus_tag="VMZ53_27910"
     CDS             complement(1789..2259)
                     /gene="sixA"
                     /locus_tag="VMZ53_27910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HUS32370.1"
                     /translation="MRIYLVRHGDAVPEDDAGSDRDRWLSPRGREAARVLGRLLRETR
                     VEPDAIISSPLPRAVQTAELLAQMLDYIGHIESWRSLEPSAHPRMAAGQLQTLGASVI
                     VVGHEPAISSMGAFILGRPSFPPFRTAQCCAIEDGKPTFMARADINVVQAYFVD"
     gene            complement(2269..3063)
                     /locus_tag="VMZ53_27915"
     CDS             complement(2269..3063)
                     /locus_tag="VMZ53_27915"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HUS32371.1"
                     /translation="MAHDVAARLGTRFEVRALNLAGRIDIRGVDPRMSPHLPVIIEVA
                     PSGYAVLLRSGVVVLFGIDPIQQERFVADLGSRIQDRYEKPEVERATVRVGDADGVEP
                     DAIIVKEVTVGRLQVIAEALGKSAILARYEEEIADAFRKIEPLAVKMRESPRRLPWKQ
                     PQLVAQIGEAILAEHQLVGSAEVTEKPDLLWDHPELDRFYIRLEDEYEIRERHHAIER
                     KLAVVSNTARTMLELSQTKRSLNVEYYIVALIVMELLISLYELVWK"
     gene            complement(3083..4606)
                     /locus_tag="VMZ53_27920"
     CDS             complement(3083..4606)
                     /locus_tag="VMZ53_27920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32372.1"
                     /translation="MLTSLTRIGLVILLLASCGKGSSNKNAKKDAGLTAPVATPIALP
                     TLGVDKIAKFNFIYDGAAVAYFKSAKDALKKSDWAEVRKQCEAALSKNPDHLDAHRLL
                     AIALAQQGEHAAAVDHLVAAIAGDYYGYGPTVADEAQLKDFLATQHGQAVTQLAAQIH
                     DEYTKRIKSSLWLVARRSPFRWPDKPGAQPGTSRGELYAFDRESKRYFRLTHTDHQVA
                     GFVRSKGAEVAVLGYDKIDHPKDDAPSQVARAWVQAYDTTEWKSATPKITLPSARVVA
                     VWYGEGDQLLVGTAPSTGRWTLGEWTISSVDKTTGKLTKVGASLGAPRIELTLDEGRV
                     VLPANPEGVTATWAGDPPRASELDVGGTKIAVPESGQANHDTIALSPGGASLAFATAV
                     DPCAKDTAPSLYIANAKTGALKHVLTAKSRFITAWLDPATLAYEDGDGAIRLWDATSG
                     REAMKLENKAGLALDVLSLAPAPLCKQEPPTAEQAGSGGDEMPPEETGSGGPVTQPQ"
     gene            4622..5185
                     /locus_tag="VMZ53_27925"
     CDS             4622..5185
                     /locus_tag="VMZ53_27925"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS32373.1"
                     /translation="MKITTLALAAVVSIAAAGCSKDKKVAKPTPEAKAAPTAAKPADG
                     KLLEQVTADQQVSQSLALSGDIVQLCGIKVAATSATPTFDYDKAELSPEDRAVLDQLA
                     TCLTTGALKGKGVSLVGRADPRGTEEYNLGLGSQRAGSVSTYLTRLGVAQTQLNVTTR
                     GALDATGTDESGWQKDRRVDITLAANG"
     gene            complement(5274..5447)
                     /locus_tag="VMZ53_27930"
     CDS             complement(5274..5447)
                     /locus_tag="VMZ53_27930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32374.1"
                     /translation="MAIVIGIVIGALVGWLIGKNKGLGGLGLILGGLLGVIGWIIVAV
                     MKGNPQGPNTPPQ"
     gene            complement(5523..5984)
                     /locus_tag="VMZ53_27935"
     CDS             complement(5523..5984)
                     /locus_tag="VMZ53_27935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32375.1"
                     /translation="MVRLFVVVTMLGLFACGGSPPPKQQSPQVRQSIARRELPADLAP
                     LVPKHGAVATGGGVKSPSFRVIVDTDAKTISSAERSRELTPRNEQYLMQLCADAWSEP
                     APATPPDKVEGYEEFLIIADGDEVFFLEGHGPITRPAAAKAIEALRAAAAL"
     gene            6038..>7042
                     /locus_tag="VMZ53_27940"
     CDS             6038..>7042
                     /locus_tag="VMZ53_27940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32376.1"
                     /translation="MALEHVAQGQTRFFTPGVAPDPRGILLGRFCLMTFPTLEGAVSW
                     LRLYSAEASLDELMANLTIAKARTALGSREIIIQIPAVSSYAADRAARLCRLVGGAIY
                     TGTAKHYVKYRDDRSPYGYDAVDIGAMPASTDFMVHGDEFNQGYVKEGELPFSKLLFR
                     LSIRKMPGADRLAAEDRGELFLAVAPGLADGIIRYLWRNRVEAEAGLFTPSAGSAFDE
                     QRRSYMWIRARNLPERILKLFIGTPGIDVFRAAGASAAVSVGYEHPIDLGSCQSVFPQ
                     DTFHVFWPGDRVDVLPGPLQLSDIGDLTRVDLEMDKPRDPAQLGGGAPDPIGVDLKLA
                     A"
BASE COUNT         1136 a         2288 c         2316 g         1302 t
ORIGIN      
        1 cgctgctcgc aatcgcggcc acggtcctgt tgcggcgagg cgcgggctcg tggcgcgctg
       61 cggggcgtcg cctcgtgcga ccgcccgtcg aagcgatctt tttcgtgccg attgcggctg
      121 tgcttatcgc cgttgcgtcg acgggaaatc cgctcgtcgg ccgcgcggtg tggggcatcg
      181 cgatcgcagg tgtcgtaacg agctggatca ccggcgcgct acttggcggc acgcgcgtgt
      241 cgctcaagcg agcggtcgcg catgcgttgt gcgcgatgct cgcggttggc gcggcgacct
      301 atgtggcggt cgatcgcggg catctcgtcg actttctcgt cgagacgtgg cgcgaaggtc
      361 ccgatcgctg atcacttacc gccggcgatc gctttcacga gctcgtacag atactgctgc
      421 ccctcgaaga atgcgctcac cgcgacgcgc tcgtccttgc cgtgaagtcg cgtgtcgagg
      481 agatcgacgg cgagaccgct gttgccgaac gtcggaatgc ccgcgccgcg caggaacacg
      541 ccatcgctcg agccgagcga catcgagggg atgacctcgg cgtccggcca gtgcttcttc
      601 gacaccgtcg cgatcgtcgc gagcagctcc ggcggcagct tcgacggacc gccgcttggc
      661 tctttacgaa caggcgtgac cgtggccccg gtaccggcga tggtttgctc gaggactttc
      721 tggacgtcct cggtggtgtg cgttgggagg atcaagcacg taagggtcgc gcgagcggtg
      781 cgtggaagcg cgccctcgtt ctcgcccgcc tcgacctgcg tcgcagcgca cgtcgtgcgg
      841 agctggccgt tgtactcggg aaggtcgcgc agcctctcga gcactgccgc cgacggtgac
      901 ggctcgagtg caccgcggag cgtggcggca atctcggggg attcgtgtct cgcgatggtc
      961 tcgatgtact gcctcgtcag ctcgcttact tcgatcggga attgcagcgc tcccagccgc
     1021 gcgagcgccg cggcgagatc gtagatggcg ttgtctttgc gaggacgtcc agcgtggcca
     1081 ccgcggttgt gaacctcgat gaagtagctc tgatagttcc gttcggcgac ctggatcgag
     1141 ttgaagatgg gcttgccgtc gcgcatgccg acccagccgc cttcgttgag agcgagctcg
     1201 gcgtcgagga gcgggcgctg gttcgcgagc agccacgaca ggcctttgcc ggtatcgccc
     1261 tcttcttcat ccgttgcgag cacgaggatg atgtcgcgat ctggcttgta gctctcggcc
     1321 ttgtatctga tgagcgccga cacgaacgcg gcggccatga acttgtcgtc ggtcgttccg
     1381 cgtccgtaaa agtagccgtc tttctcgacg agctcgaacg gatcggtgct ccagtcgctg
     1441 cgcttggcag gcaccacatc gatgtgcgcc atgacgatga tcggccgctt gcgtccggtg
     1501 cctcgatagc gcgcgacgag gttgccgcgc ttggtcccgg tctcgaagac cttcacgtcc
     1561 tcggctggaa ggcccgctgc gacgaggcga cgttccatcg catgtgcggc cttcgcggta
     1621 tcgccggtat cgctcgtgtc gatcgagatc agctcggcgt agatctcgcg agcacgcgac
     1681 tcgtttgcac gccgcgccgt ccccggatct gcagccgcgg agatacagcc ggctaccaac
     1741 aacaacacac cccagcaagc cctcatgggc ccagcctaac tcgtttcgtt agtcgacgaa
     1801 gtacgcctgc acgacgttga tatcggcgcg cgccatgaac gtcggcttgc cgtcttcgat
     1861 cgcgcagcac tgcgcggtgc ggaacggcgg gaacgacggg cgaccgagga tgaacgcgcc
     1921 catcgacgag attgccggct cgtgcccgac gacgatcacc gacgcgccga gcgtctgcag
     1981 ctggcctgcg gccatgcgcg ggtgcgccga cggttcgagg ctgcgccacg actcgatgtg
     2041 gccgatgtaa tcgagcatct gcgcgagcag ctcggcggtc tgcacggcgc gtggaagtgg
     2101 cgagctgatg atcgcgtcgg gctcgacgcg cgtctcgcgc agcaggcgac cgagcacgcg
     2161 ggcggcttcg cggccgcgcg gagagagcca acgatcgcgg tcgctgccgg catcatcctc
     2221 ggggaccgcg tcgccgtgcc gcacgagata gatgcgcatg cgtcgatgct acttccagac
     2281 aagctcgtaa agcgagatca gaagctccat cacgataagc gcgacgatgt agtactccac
     2341 attgagcgac cgcttggtct gcgagagctc gagcatcgtg cgcgccgtgt tcgagacgac
     2401 ggcgagcttg cgctcgatcg cgtggtgtcg ttcgcggatc tcgtattcgt cttcgaggcg
     2461 aatgtagaag cgatcgagct cgggatgatc ccagaggagg tcgggcttct ccgtgacctc
     2521 ggcgctgccg acgagctgat gctcggcgag gatcgcttcg ccgatttgcg cgacgagctg
     2581 cggctgcttc catggcaggc ggcgaggact ctcgcgcatc ttcacggcga gcggctcgat
     2641 cttgcggaat gcgtcggcga tctcttcctc gtagcgcgcg aggatcgcgc tcttgccgag
     2701 ggcctcggcg atgacttgga ggcggccgac ggtgacttct ttgacgatga tcgcgtcggg
     2761 ctcgacacca tcggcatcac ctacgcgcac ggtggcgcgc tcgacctcgg gcttctcgta
     2821 gcgatcttgg atgcggctgc cgaggtcggc gacgaaccgc tcttgctgga tcggatcgat
     2881 cccgaacagc acgacgacgc cggaccgcag cagcacggcg tagcccgacg gcgcgacctc
     2941 gatgatgacg ggcaagtgcg gcgacatgcg cggatcgacg ccgcggatat cgatgcgacc
     3001 cgcgaggttg agcgcgcgca cctcgaagcg agtgccgagc cgcgctgcaa cgtcgtgcgc
     3061 catactatgg gctagcaccg atttactgag gctgtgtgac aggaccgccg ctgcccgtct
     3121 cttccggtgg catctcgtcg ccgccggagc cggcttgctc agcagttggc ggctcttgct
     3181 tgcagagcgg tgccggtgcg agcgacagca cgtcgagcgc gaggcctgcc ttgttttcga
     3241 gcttcattgc ctcgcggccg ctcgttgcgt cccacaggcg gatcgcgcca tcgccgtctt
     3301 cgtatgcgag cgtcgccgga tcgagccacg ccgtgatgaa gcggctcttg gcggtgagca
     3361 cgtgcttgag tgcgccggtc tttgcattcg caatgtagag cgaaggcgcg gtgtccttcg
     3421 cacatggatc gaccgcggtc gcgaatgcga gcgatgcacc gccgggcgat agcgcgatcg
     3481 tgtcgtgatt cgcttgtccc gattcaggca ccgcgatctt cgtgccgccg acgtcgagct
     3541 ccgacgcgcg cggcggatcg cccgcccacg tagccgtgac accttcggga tttgcaggca
     3601 gtacgacacg gccttcgtcg agcgtgagct cgatgcgcgg tgcgccgagc gatgcgccga
     3661 cctttgtcag cttgcccgtc gtcttgtcga ccgacgagat cgtccactcg ccgagcgtcc
     3721 agcgccccgt cgacggtgca gtaccgacga ggagctgatc gccttcgccg taccagactg
     3781 cgacgacgcg cgcggacggc agcgtgatct tcggcgtcgc cgatttccac tccgtcgtgt
     3841 catacgcctg cacccacgcg cgtgcgacct gcgatggcgc gtcgtctttc ggatgatcga
     3901 tcttgtcgta gccgagcacg gcgacctcgg cgcctttcga gcgcacgaag cccgcgactt
     3961 ggtgatccgt gtgcgtcaac cggaagtagc gcttcgattc gcgatcgaac gcgtacagct
     4021 caccgcggct cgtgcccggt tgtgcgcccg gcttatcagg ccagcggaac gggctgcgcc
     4081 gcgcgacgag ccatagcgat gacttgatgc gcttggtgta ctcgtcgtgg atctgcgccg
     4141 cgagctgcgt gacggcttgc ccgtgctgcg tcgcgaggaa gtctttgagc tgcgcttcgt
     4201 cggcgactgt cggtccgtag ccgtagtagt cgccggcgat cgctgcgacg aggtgatcga
     4261 ccgccgcggc gtgctcgcct tgttgcgcga gcgcgatcgc gaggagacgg tgagcgtcga
     4321 ggtgatccgg attcttcgac agtgcggctt cgcactgctt gcggacctcc gcccagtcgg
     4381 attttttcag cgcgtccttc gcggacttga aataagcgac cgctgcgccg tcgtagatga
     4441 agttgaattt cgcgatcttg tcgaccccga gcgtcggcag cgcgatcggc gttgcgacag
     4501 gagctgtaag gcccgcgtct ttcttcgcgt tcttgttgct gctgccctta ccgcaggaag
     4561 ccagaagcag gatcacgagc ccgatgcgtg ttaaggaagt cagcatcaat cggaggatgc
     4621 catgaagatc accacactcg ctctcgccgc cgttgtctcg atcgcagctg ccggctgttc
     4681 gaaggacaag aaggtcgcga agcccacccc cgaagccaag gctgccccca ccgccgcgaa
     4741 gcccgccgac ggcaagctgc tcgagcaagt caccgccgac cagcaggtca gccaatcgct
     4801 cgcgctgtcc ggcgacatcg ttcagctgtg cggcatcaag gttgcggcga cctccgcaac
     4861 gccgacgttc gattacgaca aggcggagct ctcgcccgaa gatcgcgcgg tgctcgatca
     4921 gctcgcgacg tgcctgacga ccggcgcgct gaagggcaag ggcgtatcgc tcgtgggacg
     4981 cgcggatcca cgcggcaccg aggagtacaa cctcgggctc ggctcgcagc gcgcgggctc
     5041 ggtctcgacg tacctcacgc gcctcggcgt cgcgcagacg cagctgaacg tcacgacgcg
     5101 cggtgcgctc gatgcgaccg gcacggacga gtcgggctgg caaaaagatc gccgcgtcga
     5161 catcacgctc gccgcgaacg gctagcgaac tcgccaatac ataccgaaaa gcgaaacggc
     5221 caccgggtca cgaagaccgc aggtggccgt ctgcttttca cccttgtttc agatcactgc
     5281 ggcggcgtgt tcgggccctg cgggttgccc ttcataaccg cgacgatgat ccagccgatc
     5341 acgccgagca gaccgcccag gatgaggcca aggccgccga ggcccttgtt cttgccgatg
     5401 agccaaccaa cgagtgcgcc aataacgatg ccgatgacaa ttgccatgtg aatcttcccc
     5461 ctttccaaat ggtgcacaaa accgcgctga aaatctccct agatcggcat caaagatcgc
     5521 attcagaggg ctgccgcagc gcgcaacgcc tcaatagctt tcgcagccgc agggcgcgtg
     5581 atcggcccat gtccttccaa aaagaacact tcgtcgccat cggcgatgat caagaattct
     5641 tcgtagcctt cgaccttgtc gggcggggta gcaggcgcgg gctcgctcca cgcgtccgca
     5701 cagagctgca tcaaatattg ttcgttgcga ggtgtcagct cgcgcgagcg ttctgcgctc
     5761 gagatcgtct tcgcatcggt atcgacgatc acgcggaacg aaggtgactt cacaccgcca
     5821 ccagtcgcga ccgcaccatg tttcggaacg agcggcgcga ggtcggccgg caattcacgt
     5881 cgcgcgatgc tctgccgaac ctgcgggctt tgttgcttcg gcggtgggct tccgccgcac
     5941 gcaaacagac ccagcatcgt caccaccacg aacaggcgca ccatgcctgc cagtctatca
     6001 aaggccgcag gcgctctcgc gtgttaggct gcccgcgatg gcactcgagc acgtcgcgca
     6061 gggccagacg cgattcttca cgcccggcgt cgcgccggat ccgcgcggga ttctgctcgg
     6121 ccggttctgc ttgatgacgt tcccgacgct cgaaggcgcg gtgagctggc tgcggctgta
     6181 ctccgccgaa gcgtcactcg acgagctgat ggcgaacctg acgatcgcga aggcgcgcac
     6241 cgcactcggc agtcgcgaga tcatcattca gattccagcg gtctcgtcgt atgccgcgga
     6301 ccgcgcagcg cggctgtgcc ggctcgttgg cggcgcgatc tacacgggca ccgcgaagca
     6361 ctacgtcaaa taccgcgacg accgttcgcc ctacggctac gacgcagtcg acatcggcgc
     6421 gatgcccgca tcgacggact tcatggtcca tggcgacgag ttcaatcagg gctacgtcaa
     6481 agaaggcgag ctgccgttct cgaagctgct attccggctc tcgattcgca agatgccggg
     6541 cgccgatcgg ctggcagccg aggatcgtgg cgagctgttt ctcgcggtcg cgccggggct
     6601 cgccgacggc atcattcgct atctctggcg caatcgcgtc gaggccgagg ctggcttgtt
     6661 cacgccgtcg gcgggcagcg cgttcgacga gcagcgccgc tcgtacatgt ggatccgcgc
     6721 gcgcaacttg ccagagcgca tcctgaagct cttcattggt acgccgggca tcgacgtgtt
     6781 tcgcgcggca ggcgccagcg cggcggtgtc cgtcggttac gagcacccga tcgatctcgg
     6841 ctcgtgtcag tcggtgtttc cgcaggacac gttccacgtg ttctggccgg gcgatcgcgt
     6901 cgacgtgttg cccggcccgc tccagctctc ggatatcggc gatctgacgc gcgtcgatct
     6961 cgagatggac aagccccgcg atcctgcgca gctcggcggc ggcgcgcccg atccgatcgg
     7021 cgtcgacctc aagctcgcag cc
//
LOCUS       DATLBZ010000560        20554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10059869, whole genome shotgun sequence.
ACCESSION   DATLBZ010000560 DATLBZ010000000
VERSION     DATLBZ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20554
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10059869"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VMZ53_27945"
     CDS             <1..265
                     /locus_tag="VMZ53_27945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32377.1"
                     /translation="VAEIEAEKPTTFTVRESWHPRWHAYLDGNEVPVRRVTPDFPAID
                     VPPGKHVLEMRFERPWWMLASWLAWPLISLGAWFVLRRRRPGA"
     gene            complement(425..2044)
                     /locus_tag="VMZ53_27950"
     CDS             complement(425..2044)
                     /locus_tag="VMZ53_27950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32378.1"
                     /translation="MSSESDDTKKKRDAAEADVDAGSTVSVNLADIPGAHPATASGDP
                     LPELPAAAEPPAAQPPVAKPPAAEPSAAVPPTAERATGASGSINVEVSSPELPSSSSS
                     GSVDVAVSSPAIPEAVGALESQPVSGSGRVVTAVAAANEGTGPARRARRVTGLVQDAV
                     SAGVEKLGAGIESVGEGVTKVGDLTKKVPLVGAGVGKLGEGLTKAGESIHALPRVAQT
                     RRGRLLVRSVVVGFLLVFSWITVIVGFQLRQHDTPDFRPSAEQILLQISQGKTSIGDL
                     YERASPRFQELVRKEQFIDNMMDLNATNGAFREITAINETLVTIGPTGRVGRVSLTAS
                     YEKGIAHGSISFHYDKGQWKLLGIGVEVPRDVRITETEREKRVAACKDAKGHDVSDQR
                     AECPVRDAFETILEKIRDGNAGIVWDEASDIFKQQETRTRFIQVQADTRAAIGDYKRL
                     LNVTEAKSIGGLTATFIAVAEFERSSGVRVDADFARLRQTDKWQLRRFKIVVPMPRAE
                     DDPARPNLVPPAPQMPPPPREATSDASSSTP"
     gene            complement(2159..3004)
                     /locus_tag="VMZ53_27955"
     CDS             complement(2159..3004)
                     /locus_tag="VMZ53_27955"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HUS32379.1"
                     /translation="MTEYIRPRDVAEALAARAAHPDWMVLAGGTDLMVNANHRPVPAG
                     IIDLWRLPGLGTITAGERSITIGAGATWYEVENHPAVLEKWLPLALAAREIGALQIQM
                     RGTVGGNVGNSSPVGDSLPVLLALDAELELASARGRRKLPYSQFCTGYRKTALAPDEL
                     IISVTLLAPTASTRTMWRKVGTRRAQSISKVMGAAAITLDGGVVSSARVALGAVADRP
                     IRVTAVEDAVRGLTPAHAADAARTALRTAIRPIDDVRSTASYRHDVAENLVARFFSSL
                     TTGVS"
     gene            complement(3001..3555)
                     /locus_tag="VMZ53_27960"
     CDS             complement(3001..3555)
                     /locus_tag="VMZ53_27960"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HUS32380.1"
                     /translation="MKITMNVNGAEMSADVSPWKRLLDVLREDLRLTGTKEGCGEGEC
                     GACSVLLDGEVVNSCLVAAAQCSGRTLRTVEGLAQGEVLHAVQQQLVKCGGAQCGICT
                     PGIAICASHVVDRQLLRGKSEVDARATVRELLAGNICRCTGYQLIVEAVIAAAHDITG
                     VSSNATGVSSNDASELRSRDEGRR"
     gene            complement(3552..5750)
                     /locus_tag="VMZ53_27965"
     CDS             complement(3552..5750)
                     /locus_tag="VMZ53_27965"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HUS32381.1"
                     /translation="MERVPQARPPVGTSLPRVDGVAKVTGRARYLDDLEAPGAWFGAT
                     VRSAVAHGVLEAIDLDPAFDWSQVVVATAADIPGKNVIALIEDDQPALVAIGARVMHV
                     DEAIALVAAPTKALAMAAAKAVKPRIRQLPAVFTVEDSLAVKQKLYGDTNVFKQFLIR
                     KGHASDAEFEAALAKAAQVIDGTYYTSPQEQMYIEPQAMMAEWQDGRCYIVGSMQCPY
                     YVHKAMKPLLGCDADGVVITQSVTGGGFGGKEEYPSMLAAHVALLAHKAQHAVKIVYD
                     RDEDIAATTKRHPAFTRHRLAVDAAGEIVAIDVDVVMDGGAYLTLSPVVLSRGVLHAA
                     GPYRCPVVRVRGRVVATNHPPHGAFRGFGAPQTTFAYERQIQKLAIARGEDPLALRKR
                     LALRPGDTTATGQELGFSVGTDEVIAAIERVAGSPPAKDATRANGSPIRRGRGVIFYF
                     HGAGFTGSGESRLAGRATVARTVEGRYEVRSSSTDIGQGAITIFTQIAASSLGVDPSL
                     IDVIDPTTALVPDSGPTVASRTCMVVGGLVERAARELREKLATAPGGEEVAVTHQYEP
                     PPGIHFDDATYTGAAYPCFGWAACLVDVSVDLDTYEVSVDRCVQAIDVGKAINPHIVR
                     GQIDGGTLQALGWALLENVIYKDGKVVNANMTNCIVPTFADAPELETIIVEVPYPFGP
                     SGAKGVGEIPMDGPAAAVANAVENALGCAFDALPISPEAIATSKPRWRLS"
     gene            complement(5865..7283)
                     /locus_tag="VMZ53_27970"
     CDS             complement(5865..7283)
                     /locus_tag="VMZ53_27970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32382.1"
                     /translation="MRTLVVLPLLLAACGEVDPVRHLDGGMIDSPEMIDAPVTPQPVT
                     LTIMRAGLPQVGIVVHFQNADSTLVATAMTDATGTASQIMNAGGYVTAIDPFATASGQ
                     VQYPVRTFAGVKPGDHLRLVADDGTAFVMTATLPLETDPAINHYYVHSSCGDSYLGSA
                     GSGNTPSGNAYFYDCGATTDFLVTAEDGNFNVLSSFFVAGQAVSSGGTLDFSTKTFTA
                     PTARTYTFSNVPNGASVTVRDHLASTKGELIEVGGDVSGDPATGTLNVPAFANAVSIV
                     EGQVYDSLTIHSSVDWGPVTSTFSVADYSARMLVGFESTPAFDATTHKITWTATSGVA
                     PDFTYARGRVYRQAFGREIDWQLVAPYTAGTVTYPVVPAGTTDINFTASDSVSLEVGH
                     VKLPPGGYDAVRANVFVADGPIGLVTTATGTAQLALSSNNLARTIKQPSRWFSHRASS
                     ADAELATPARPSMKQTLSGRRR"
     gene            7362..8732
                     /locus_tag="VMZ53_27975"
     CDS             7362..8732
                     /locus_tag="VMZ53_27975"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUS32383.1"
                     /translation="MRAIGAGDVSLSRSAARGVDSHDLSRAVHATFEAGIDVVDIAPE
                     EDSERLVGEVIRALHLRDRALPITRVPALVPTTSEGWTTRDPLREQLPHRYLQERIER
                     SLRATRLDALPVVMIELRAAWRESPVWPELVDACARLVRDGKVLAYGAYIDQIEEDST
                     ALLDETWLDAVNVPYSLCERAAERVIARVLELRVRPSDEAAGATGGPSGSGVARETSD
                     ERDRNAATRDRASPQSPSTESLANADPALAAAAASDPNIAAVLASSLTGVSDSGLLTG
                     VNSSSSSRTGVSFLDPKMAAKDPVVAAALARDPKLAAALGIELPRRDGARDVAILVRR
                     PLAGGALAGTLGPGVKLRKDDDRKLDARTLERIAVAVAKLAAYVKETPPAARSCDAAK
                     LQLERNEKREDVECVTLAELALRFAIDRGTMPLVRLQHRDHIVDALAAMVARPLSREL
                     TPNLDI"
     gene            8757..9791
                     /locus_tag="VMZ53_27980"
     CDS             8757..9791
                     /locus_tag="VMZ53_27980"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HUS32384.1"
                     /translation="MKSRPVENPPVRFRDVTVTYEDGDGPPPATVTLLQDQSRSILSR
                     NDSPDLDFRWSANPYRGCLHACSYCYARPNHEYLDLGAGTDFDTKIVFKPRAAELLRE
                     AFDKPSWKGELIMFSGVTDCYQAVEKDLELTKQCLEVCLEYRNPVSIITKSALVERDV
                     DLIAELAREAGLSLNVSLAWLDAELARTIEPWAAAPQRRLKVIETFAKAGVPVGIMMA
                     PIIPGLNDNQIVKLLEAAREAGAQWAGWSLLRLPGAVKEVFEDRLRVSLPLVADRVMN
                     RVRDTRGGNKLYDATFGVRGRGVGVYAQTIASLFDTTAKRLGFGDNDEPDPPSRFRRP
                     PKPTNQLALF"
     gene            complement(9873..10898)
                     /locus_tag="VMZ53_27985"
     CDS             complement(9873..10898)
                     /locus_tag="VMZ53_27985"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS32385.1"
                     /translation="MSTLLRIDVAEIGSPPLPPIHLAPPFVIGSSLTARVRLPETAAT
                     SEHVKVDAGGRWVALAPVRWRDAAKKSAAADDDTGSEAAAAELPGDATVAEGVVWQGV
                     VIQIASYRVTIGPAPSGATPTPPQRTESLARELLRNLLGSAAAPTLEITSGPLAGAKR
                     PLAAPESTLVIGRGDEANWIIDDGDLSRAHVEIRRTWDGTFITDLDSKNGTKINGKRI
                     TAAPSPRESRTDSATALHDGSRIELGKLAFIFRDPAEKHLPPPIDRPATSAAESRSDD
                     HTERGARTASVDNSASRGEERSSVLRSSQRANAPSSERRASTAPFYVAAAIVVLALAG
                     LVWVLSS"
     gene            complement(10990..11643)
                     /locus_tag="VMZ53_27990"
     CDS             complement(10990..11643)
                     /locus_tag="VMZ53_27990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HUS32386.1"
                     /translation="MRRALTSLMLLLAFGTLARADIYSYTDDEGVVHFTNLKPSGGGK
                     WKKVLDSAPPKDSKAAVDRGPRYDKVGATDHSPERFHRYDQYIYEASELYRIPVPLIR
                     AVIKTESDFDPHVVSGLDCKGLMQVHPAVQLDMGAQGDIFDPRTNIMTGTRLLRYLAN
                     RLDGDLVLTIAAYHAGLGSLAKFGYAVPPYTYTRQYLKMVLERYYQYKTQETQQRVQ"
     gene            11703..13289
                     /gene="nadB"
                     /locus_tag="VMZ53_27995"
     CDS             11703..13289
                     /gene="nadB"
                     /locus_tag="VMZ53_27995"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HUS32387.1"
                     /translation="MAEILETDYLVMGSGIAGLNFALLAAEHGRVVVVTKKRPADTNT
                     NWAQGGVAAVLGPDDSFESHIADTLKAGDGLCDPSTVEIVVRDAPREVQRLLDLGVRL
                     ARDERGNLDLGLEGAHSKHRVVHWEDITGREIQRALIAAVEKHPNITMLDSHIGVDLL
                     SMAKYGGDPACFGAYVLDVRSGEVKTICARATVLATGGTGKVYIYTSNPDVATGDGVA
                     MAYRIGAACGDLEFTQFHPTVLYHPLAGSFLISEALRGEGGVLKNARGEEFMDYHPMK
                     SLAARDIVARAIDNELKKSGADCVYLDMTHLDPAMVKGRFPNIYERCLSLGIDITKQG
                     IPVVPAAHYMCGGIKTNHDGATTIQGLYAIGECAFTGLHGANRLASNSLLEGMVFSTR
                     AAAAVREAPRIRPSQVASWSYGGATDSNDAIVVSLNWEEIRRFMWSYVGIVRSDKRLE
                     RARRRIEILRDEIREYYIDFKVTADLVELRNLALVAHLIIESARRRKESRGLHYTLDY
                     PDKRPEALHSEIQLTNGPRS"
     gene            13643..14371
                     /locus_tag="VMZ53_28000"
     CDS             13643..14371
                     /locus_tag="VMZ53_28000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32388.1"
                     /translation="MQRLALVLALALAGGGAAAGCHRARGPASPASDGVREYLKALRS
                     NDPHDAYDLLASDVRKKISYSEFALQWKQSEAERKWQAKVLEDSLKGNPDVGERALIS
                     FSDGKLVQLEREGKTWRLESELVSRSRAKRPRDAIRMFADAIAARDVGAALNVLTQRR
                     REGLSKQIEGFISGIAKHASGPLDEQADRAELRWDENGIRYRIVLRKEDDEWRVDDIY
                     IRPAPKDEDTTKPGDIEGLPGDDF"
     gene            complement(14477..14977)
                     /locus_tag="VMZ53_28005"
     CDS             complement(14477..14977)
                     /locus_tag="VMZ53_28005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32389.1"
                     /translation="MTRIAILALAVGCGSTTPPPTAHETLGTSHAASHAVAPRSPWSR
                     ERIERELGDNALPVLRELARGPIVVGDIDAGTTQVVCGERATTALAQTSASFASPIHC
                     SQSNDRMTVCLTAESSSPMAIAIFVRGDEWALAAVAVSKHYDAKAAHAAVVTTGQLLA
                     NGVACP"
     gene            complement(14974..16128)
                     /locus_tag="VMZ53_28010"
     CDS             complement(14974..16128)
                     /locus_tag="VMZ53_28010"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HUS32390.1"
                     /translation="MAHKDADEPFSAGSVARQLDYCTYCPKMCRHACPVSTASGRETY
                     IPQVKMDRMNQLRLGRSRWTEESTDPLYACTGCRHCTMYCDHGNEPGLVLFTGRAAAV
                     EHGTQHPALEGYSERFRAREQRLSAQLAAGFSEHLATEGDVGFWPGCDAIDKGAADIA
                     AAMALFERVGLETVKIVSAPQACAGYPLLAAGHVDLFRWHAGRVASAMRGFRKVAMNC
                     SACLYAVRAQYAAEGVSVRTEVVSLADLLAPAANNLACSLNRKSVYYHDPCYNARYNN
                     VVEAPRRALAGLAELREFSWNRADTECCGGGGLLPKTMPTVADQMAKRRLAEVQASGG
                     GMVVTSCATCTFMLKRNAPPSVEVANLATAMAQLSETPFTAPPSSPVDDE"
     gene            complement(16149..16829)
                     /locus_tag="VMZ53_28015"
     CDS             complement(16149..16829)
                     /locus_tag="VMZ53_28015"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HUS32391.1"
                     /translation="MARDDFSLDHAALLIGAWDYPERDLERYREMLDEISARCAPDVA
                     RAPNGIGRARAISEHLFDRLGFSGNNSDYYDPRNSFLCEVIDRRQGIPISLSVVYLEV
                     ARRVGVLAQGVNFPGHFLVRVAIEDAWLFLDPYTHGRTLTPADLEELLRKTTTPNAIL
                     EPSVIAAASKRQIVARMLVNLAGIYGRNGDLPRSLDVLERLAVLEPSNPRIARDLAQL
                     RERVDGLN"
     gene            16940..18076
                     /locus_tag="VMZ53_28020"
     CDS             16940..18076
                     /locus_tag="VMZ53_28020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32392.1"
                     /translation="MAEEPRPGLIVGGRYELIEVAGRGGMADVWRGRVRGDWGFTRDV
                     AIKQMHQNLASQPPYVAMFVEEARLGSMLHSPNVAEVHDFVHDRGNYYMVLEWIDGVD
                     LGTWIRWHASRNEQTRWELVAAVAVGILRGLAAAHERTAPDGTPMPVVHRDVSPHNVL
                     LTNRGLVKLIDFGLALAPDRPQETTEPGIVKGKMSYLAPEIVGGKRPMAESDQFACGS
                     VLWEALVGRKLFDGATDYEVYCRLRDCMVQPLRPLRPDVPAAFIQLINRALSANPDAR
                     FPTVREMARQIGTTLKKVQLRKDLHSVLSKSVADAKQGMGLGPRTGDPSSATPVAQIV
                     GDDTPRIELAPRIDTPVEGASVGALEKLRGLRHRLPSFFGRKRG"
     gene            18080..>20554
                     /locus_tag="VMZ53_28025"
     CDS             18080..>20554
                     /locus_tag="VMZ53_28025"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HUS32393.1"
                     /translation="MRRLALLLIFAACGDNAAPPFVPLQSGNALITLDDGALVLSRDG
                     VEKTRLAPSAFQIGTVDDLDSGASFDPYWLWVDNAPEPPEGLAWHAASSLRVERSDTQ
                     GFALSLDDVGNVECVPSTPGSFRLVFSSSRTRVAFLRVQPAVNDTERFYGLGEWGDAV
                     EHRGKLRPMQLEVDTTSESATNEAHVPVPLAISTSAWGVFAQSKRPGAFDVARMDPAK
                     LDIAFGTADDSARGLTFHLFTAPDGEPLGVLQPYFEVTGYPALPAYWAYGPLLWRDET
                     TGQVQTLDDIQQIRTRDLPTSGIWFDRPYASGVQTFDWDPAKFAAPADMLHALHDAGL
                     RYAIWQAPYVAPANNDQDPAPDQNAYATAHHFFPPTSGIQANQWSKPIDFTNPEAYAW
                     WQANLKKYTDPLSLGGWGVEGFKLDYGEDIVLGALGQRVPWVFADGSDERTMHVDYTL
                     LYHGIYRDMLDADGGFLLTRTGRWGDQTRGMIVWPGDLQADLSKFGDSKPGSSSKTVG
                     GLPTALAFGIGLSASGFPFYASDTGGYRSSPPNNETWLRWVEANAVWSAMQVGDSSSQ
                     MPWEYTTANGRSDASLDTYREYARLHLRLFPYVWSLAQQMRATGYPIVRPLGLAYPGM
                     PEHPADQYLVGDALLVAPVITAGATARTAWFPRGTWIQWFTGEEFTVAAVEQRSVAAA
                     LDELPLYIQRGAIVPMLRDTIDTLAPATVSGIESYANDPGLLVVRIAPGPHSAFTLFD
                     ETKVEQQLEAGVTTVDYTPGKTFVAGALLESIATDAPAAVTTESGPLTQRASYAALQA
                     AADGWFYDATATGGTLWIKLAGAATVT"
BASE COUNT         3424 a         6706 c         6849 g         3575 t
ORIGIN      
        1 cgtcgcggag atcgaagccg agaaaccgac gacgttcacg gtacgcgaga gttggcaccc
       61 gcgctggcat gcgtatctcg acggcaatga ggttcccgtt cgtcgcgtta ccccggactt
      121 tcccgcgatc gacgtgccgc ccggcaagca tgtcctcgag atgcgcttcg agcggccgtg
      181 gtggatgctc gcgtcatggc tcgcttggcc actgatttcg cttggagcgt ggttcgtgct
      241 tcgtagacgc agacctggtg cgtagcggaa cggtcgtcac ttcggcaggg aagagcgcaa
      301 cgcggtgagg cgatcaactt accccggtaa ggtgatcaac ttaccccggt cgtcgaaatg
      361 cgacgtaagt gcgcgatgca gcgtcgtcgc aacttacccc ggtgctcgat cgcgttctga
      421 cgtcctacgg cgttgacgag ctcgcatcgc tcgtcgcctc gcgcggtggt ggcggcatct
      481 gtggcgccgg agggacgaga ttcggacgcg cgggatcgtc ttcggcgcgc ggcattggca
      541 cgacgatctt gaagcggcgc agctgccatt tgtccgtctg tcgtaggcgc gcgaagtcgg
      601 catcgacacg cacaccgctc gagcgctcga attcggcgac ggcaatgaac gtcgcggtga
      661 gcccgccaat tgacttggcc tccgtcacgt tgaggaggcg cttgtagtcg ccaatcgctg
      721 cgcgcgtatc tgcttgcact tgaatgaagc gcgtccgcgt ttcttgctgc ttgaagatgt
      781 cgctcgcttc gtcccagacg atgccagcgt tgccgtctcg gatcttttct agaatggtct
      841 cgaacgcatc gcgaacggga cactctgcgc gctggtcgga cacgtcgtgg cctttggcgt
      901 ccttgcaggc ggcgacgcgc ttctcgcgtt cggtctcggt gattcggaca tcgcgcggaa
      961 cttcgacgcc gatgccgagc agcttccatt ggcccttgtc gtagtgaaag ctgatcgagc
     1021 cgtgcgcgat gcctttttca tatgacgccg tcaggcttac gcgcccaaca cggcccgtgg
     1081 gaccgatcgt cacgagcgtc tcgttgatgg ctgtgatctc gcggaatgcg ccgttcgtcg
     1141 cgttgaggtc catcatgttg tcgatgaact gctccttgcg aacgagctct tgaaaacgcg
     1201 gcgacgcgcg ttcgtagaga tcgccaatgg atgtcttgcc ttggctgatc tgaaggagga
     1261 tttgctcggc ggatggacgg aagtccggcg tgtcgtgttg ccgaagctgg aagcccacga
     1321 tcaccgtgat ccacgaaaac acgagcaaga aaccgacgac gacgctgcga acgagcagcc
     1381 gaccacgccg ggtctgcgcg acgcgcggca gtgcatgaat cgactcgcca gccttcgtga
     1441 gtccttcgcc gagcttgccg acgccggcgc cgacgagcgg caccttcttt gtcaggtcgc
     1501 cgactttggt aacgccctcg cctaccgatt cgataccggc gccaagcttt tcgacgcctg
     1561 cgctcacggc gtcttgaacg aggcccgtca ctcgtcgcgc gcgtcgcgcc ggtccggtcc
     1621 cttcgttggc tgcagcaacc gctgtgacta cgcgcccgct gccggacacc ggttgcgact
     1681 cgagcgcacc gaccgcctcg gggatcgctg gactcgagac cgcgacgtcg accgatccgg
     1741 aggacgagct cgaaggaagc tcgggactgg atacttctac attgatcgag cccgacgcgc
     1801 cggtagctcg ctccgcagtc ggcggcacgg cggcggatgg ctcggcggct ggcggcttgg
     1861 caactggcgg ctgggcggct ggcggctcgg ccgctgcagg gagctccggc aacggatcgc
     1921 cagacgctgt ggccggatgc gcacctggaa tgtccgctag gttgaccgac acggtcgagc
     1981 ccgcgtcgac gtcggcttcc gcggcgtctc gcttcttctt cgtatcgtca gattccgagg
     2041 acacctctgg agtctcgcga gcttaactta ccccggtagt aatgcccgtg gcgtgcgctc
     2101 tgacggtgaa gtctcgcgcg tttaacttac cccggtcgtc gcgttccggt cgtcgcgttt
     2161 aacttacgcc ggtcgtgagc gagctgaaaa agcgcgcgac caggttctcc gcgacgtcgt
     2221 ggcgatacga cgcggtagag cggacgtcgt cgatcggacg aatcgcggtg cgcagtgctg
     2281 tccgcgcagc gtccgcagcg tgggccggag tcaggccgcg gactgcgtcc tcgactgcag
     2341 tcacgcgaat cgggcgatcg gccaccgcac caagagcgac gcgtgcggat gaaacaacgc
     2401 caccatcgag cgtgatcgcc gctgcgccca tgaccttcga aatcgactgc gcgcggcgcg
     2461 tcccgacttt gcgccacatg gtacgcgtcg acgccgttgg agccagcaac gtcacggaga
     2521 tgatcagctc gtctggcgcg agcgcggtct tgcgatagcc cgtgcaaaac tggctgtacg
     2581 gcagcttgcg acggcctcgc gcgctagcga gctcgagctc cgcatcgagc gcgagcagca
     2641 ccgggagcga atcgccaacc ggcgacgagt tgccgacgtt gccgccgacc gtgccgcgca
     2701 tttggatctg tagcgcaccg atctcgcgag ccgcgagcgc gagtggaagc cacttctcga
     2761 gcaccgccgg atgattctcg acctcgtacc aggtggcgcc cgcgccgatc gtgatcgagc
     2821 gctcacccgc ggtgatggtg ccgagtcccg gcagccgcca tagatcaatg atgccggcgg
     2881 gcacgggacg atggtttgcg ttcaccatca ggtccgttcc acccgcgagc accatccagt
     2941 ccggatgagc tgcgcgcgcc gcgagagctt ccgcgacgtc acgcggccgg atgtactcgg
     3001 tcatcggcga ccctcgtcac gtgaacgcag ctcgctcgcg tcgttcgaac ttaccccggt
     3061 agcgttcgaa cttaccccgg tgatgtcgtg agccgcagcg atcacggctt cgacgatcag
     3121 ctggtagccc gtgcatcggc agatgtttcc ggcgagtagc tcgcgaaccg ttgcgcgcgc
     3181 gtccacttct gacttacccc ggagaagttg gcggtcgaca acgtgcgacg cgcagatcgc
     3241 gatgcccggt gtgcagatgc cgcattgcgc tccgccgcat ttgacgagct gctgttgcac
     3301 ggcgtgaagc acctcgcctt gcgcgagtcc ctcgaccgtc cggagtgtcc ggccactgca
     3361 ttgcgccgcg gcgacgagac acgagttcac gacttcgcca tcgagcagca ccgagcatgc
     3421 gccgcactcg ccttcgccgc atccctcttt cgtgcccgtt agccgaaggt cttcgcgcag
     3481 cacgtcgagc aggcgtttcc acggcgagac gtcggccgac atttccgcgc cgttgacgtt
     3541 catcgtgatc ttcacgagag cctccagcga ggcttgctcg ttgcgatcgc ctccggcgag
     3601 atcggcaatg catcgaacgc acagccgagc gcattttcga cggcgtttgc caccgcggct
     3661 gcgggcccat ccatcggaat ctcgccgaca cccttcgcgc cgcttggccc gaacggatac
     3721 ggcacctcga cgatgatcgt ctcgagctcc ggcgcgtcgg cgaacgtcgg gacaatgcag
     3781 ttcgtcatgt tcgcgttcac gaccttgccg tctttgtaga tgacgttctc gagcagcgcc
     3841 cagccgagtg cttgcaacgt gccgccgtcg atttgaccgc gaacgatgtg cggattgatc
     3901 gctttgccga cgtcgatggc ctgcacgcag cggtcgaccg acacctcgta ggtatctaga
     3961 tcgacggaga catcgacgag gcacgcagcc catccgaagc acgggtacgc agcgccggtg
     4021 tacgttgcat cgtcgaagtg gatgccgggc ggcggctcgt actgatgcgt caccgcaact
     4081 tcttctccgc ccggtgcggt cgccagcttc tcgcgcaatt cacgcgccgc gcgctcgacg
     4141 agcccgccga cgaccatgca cgtccggctc gcgaccgttg gcccactatc gggcacgagc
     4201 gcggtcgtcg gatcgatcac gtcgatcagc gacgggtcga cgcctaaaga gctcgcggcg
     4261 atctgcgtga agatcgtgat ggcgccctgc ccgatgtccg tcgagctgga gcgcacttcg
     4321 tagcgacctt cgacggtgcg agcgaccgtt gctcgtccgg ccagccgcga ctctcccgag
     4381 cccgtgaaac ctgcgccatg gaaatagaag atgacgccgc gcccacgccg gatcggcgag
     4441 ccgtttgcgc gcgtcgcatc tttcgccggc ggtgagcccg cgacgcgctc gatcgcagcg
     4501 atgacttcgt cggtaccgac ggaaaagccg agctcttggc ccgttgccgt cgtgtcgccc
     4561 ggccgaagag cgagccgctt gcgcagcgcc agcggatctt cgccgcgtgc gatcgcgagc
     4621 ttctggatct gacgttcgta cgcaaacgtc gtctgcggtg cgccgaagcc gcgaaatgct
     4681 ccgtgcggcg gatgattcgt cgcaacgacg cgcccgcgga cacgcacgac cggacaccga
     4741 tacggcccgg cggcgtgcag cacaccgcgc gacaggacaa cgggcgagag cgtcagatag
     4801 gcgccgccgt ccataacgac atcgacgtcg atcgcgacga tctcgccggc agcatccacc
     4861 gcgaggcgat ggcgcgtaaa tgcgggatgt cgcttcgtgg tcgccgcgat gtcctcgtcg
     4921 cggtcgtaga cgatcttcac cgcgtgctgc gccttgtgtg cgagcagcgc gacgtgtgcg
     4981 gcgagcatcg acggatactc ttctttgccg ccgaacccac cgcccgtgac cgattgcgtg
     5041 atgacgacgc cgtcagcatc gcatccgagc agcggcttca ttgccttgtg gacgtaatac
     5101 ggacactgca tcgacccgac gatgtagcaa cggccgtcct gccactccgc catcatcgcc
     5161 tgcggctcga tgtacatctg ctcttgcggc gacgtgtagt aggtcccgtc gatcacctgc
     5221 gcggcctttg cgagcgcggc ctcgaattcc gcatcgctcg cgtgcccctt gcggatgaga
     5281 aactgcttga agacgttggt gtcgccgtag agcttctgct tcacggccag tgaatcttcg
     5341 acggtgaaca cggccggcag ctgccgaatg cgcggcttca ccgccttggc cgcggccatc
     5401 gcgagtgcct tcgtcggcgc cgcaaccagc gcgatcgctt catcgacatg catgacgcgt
     5461 gcaccgatgg cgacgagcgc cggctgatcg tcttcgatca gcgcgatgac gttcttaccc
     5521 gggatgtctg cggcggtcgc gacgaccacc tgcgaccagt cgaacgcggg atccagatcg
     5581 atcgcctcga gcacgccatg cgcgaccgcc gaccggaccg ttgcgccgaa ccaggcaccc
     5641 ggcgcctcga ggtcgtcgag gtaacgcgct cgcccggtga ccttggcgac accatcgacc
     5701 cgcggaagtg acgtgccgac aggcggacgc gcctggggta cccgttccat cggtcgattc
     5761 aaccacatag gacgggagcg ccgccacgac ggggagccgc actcttcgtc gcacgccggt
     5821 caccgcgtcg agcgcgaatt cttctgtggc ggcgagacct tccgtcagcg acggcggccg
     5881 gacagcgtct gcttcatcga cggccgcgcc ggcgtcgcga gctcggcatc cgcggacgat
     5941 gcgcgatgcg agaaccagcg cgacggctgc ttgatcgtcc gcgcgaggtt gttagagctc
     6001 agcgcgagct gcgcggttcc cgtagcggtg gtcacgagcc cgatcggacc atcggcgacg
     6061 aacacgttgg cgcgcacggc gtcgtaaccg cctggcggca gcttcacgtg tccgacttcg
     6121 agactgacgg agtcggatgc cgtgaaattg atatcggtag tacccgcggg cacgacggga
     6181 tacgtgacag tgccggcggt gtacggcgcc accagctgcc aatcgatctc gcggccgaag
     6241 gcctgacgat agacacgtcc ccgtgcgtac gtgaaatcgg gcgcgacgcc ggaggtggcg
     6301 gtccacgtga tcttgtgcgt cgtggcgtcg aaagccggcg tcgactcgaa cccgacgagc
     6361 atgcgggccg agtagtcggc cacggagaac gtcgacgtga ccggacccca gtcgaccgag
     6421 ctgtggatcg tgagcgagtc gtacacctgg ccctcgacaa tgctgacggc attcgcgaac
     6481 gcgggcacgt tgagtgtgcc agttgccgga tcgccgctca cgtcgccgcc gacctcgatg
     6541 agctcgccct tcgtggaggc gaggtgatcg cgaaccgtga ccgacgcgcc gttcgggacg
     6601 ttcgagaacg tgtacgtgcg cgcagtcggc gctgtgaagg tcttcgtcga gaagtcgagc
     6661 gtgccgccac tggagacggc ttgtccggcg acgaagaagg acgagagcac gttgaagttg
     6721 ccgtcctccg ccgtgacgag gaaatcggtc gttgcgccgc aatcgtagaa gtacgcgttg
     6781 cccgagggcg tgtttcccga gccagcgctt ccgagatagc tgtcaccgca cgacgaatga
     6841 acgtagtagt ggttgatcgc tggatcggtc tcgaggggca gcgtcgccgt catcacgaac
     6901 gccgtgccat cgtccgcgac gaggcgaagg tgatcgcccg gcttcacgcc ggcaaatgtc
     6961 cgcacggggt actgcacctg gcccgacgcc gtcgcgaacg gatcgatggc cgtgacgtac
     7021 ccgccggcat tcatgatctg cgatgcggtg cccgtcgcat ctgtcatcgc ggtagcgacg
     7081 agcgtggagt cagcgttttg aaagtgaacg acgattccaa cctgcggaag cccggcgcgc
     7141 atgatcgtga gcgtcaccgg ttgcggcgtc acgggggcgt cgatcatctc gggcgagtcg
     7201 atcattccgc catcgaggtg gcgaaccgga tcgacttcac cgcaagcggc caatagcagg
     7261 ggaagcacaa caagcgtacg catggagtcc atgcatcgca cgagcgagct gccgagcaca
     7321 atcgggtagg attgccgtcg atgtggccac gatcactagc agtgcgagcg atcggcgctg
     7381 gcgacgtcag cctctctcgt tcggccgcgc gcggtgtcga ctcgcatgat ctttcgcgcg
     7441 cggtccacgc gacgttcgaa gcaggtatcg atgtcgtcga cattgcaccg gaagaagaca
     7501 gcgaacgctt ggtcggcgag gtgatccgcg cgcttcatct gcgagaccgt gcgctaccga
     7561 tcacgcgcgt accggcgtta gttccgacaa cgtcggaagg ctggacgacg cgcgatccgc
     7621 tgcgcgagca gctaccccat cgttacctcc aggagcgcat cgaacgctcg cttcgcgcga
     7681 cgcgcctcga cgctctaccg gtcgtgatga tcgagctgcg agcggcgtgg cgcgagtcgc
     7741 ccgtgtggcc ggagctggtc gatgcgtgcg cgcggctcgt gcgcgacggc aaggtgctcg
     7801 cgtacggcgc gtacatcgat cagatcgaag aagacagcac ggcgctgctc gatgagacgt
     7861 ggctcgatgc agtcaatgtg ccgtactcgc tctgcgaacg cgcggcggaa cgggtgatcg
     7921 cgcgagtgct cgagcttcgc gtccgaccgt cggacgaagc agctggggcg accggagggc
     7981 cgagcggtag cggcgttgct cgggagacat cggatgagcg cgaccgcaac gcagcgactc
     8041 gcgatcgtgc atcgccgcaa tcaccttcta cggaatcgct agcgaacgcc gaccctgccc
     8101 tcgccgctgc ggccgcgagc gaccccaaca tcgcggcagt actggcctct tcacttaccg
     8161 gggtaagtga tagcgggcta ctcaccgggg taaattcgtc gtcgtcttca cgtaccgggg
     8221 taagttttct cgatccgaag atggccgcga aggacccggt cgttgctgcg gcgttggcgc
     8281 gcgatccgaa gctcgcggcg gcgctcggca tcgagcttcc gcgacgcgac ggcgcacgtg
     8341 acgtcgcgat tcttgtgcgg cggccactcg cgggtggtgc gttggcggga acgctcgggc
     8401 ccggcgtgaa gctgcgcaaa gacgacgacc gaaagctgga cgctcggaca ctcgaacgca
     8461 ttgcggtcgc cgtcgcgaag ctcgcagcgt atgtgaagga gactccgccg gccgcgcgct
     8521 catgcgatgc cgcgaagctg cagctcgagc gcaacgagaa acgggaggat gtggagtgcg
     8581 tgacgcttgc cgagctcgcg ctgcggttcg cgatcgatcg cggcaccatg ccgcttgtcc
     8641 gattgcagca ccgcgatcac attgttgacg cgctcgcggc catggtcgcg cggccgctct
     8701 cgcgagagtt gacgccgaat ctcgacatct gaccatccgt tcagtatgct gtgcgcgtga
     8761 agtctcgccc cgtcgagaat ccgccggttc ggttccgcga cgtgaccgtc acgtacgagg
     8821 acggcgatgg cccaccgccc gcaaccgtca cgctgctcca agatcagtcg cgatcaattc
     8881 tctcgcgcaa cgactctccc gatctggact tccgctggag cgcgaatccg taccgcggat
     8941 gtctgcacgc gtgctcgtat tgctacgcgc ggccgaatca cgagtatctc gacctgggcg
     9001 cgggcacgga cttcgatacg aagatcgtct tcaagccgcg tgcggcggag cttctgcgcg
     9061 aagccttcga caagccgtcg tggaaaggcg agctgatcat gttcagcggc gtcacggatt
     9121 gttatcaggc tgtcgagaag gatctcgagc tcacgaagca atgcctcgag gtctgcctcg
     9181 agtatcgcaa ccccgtctcg atcattacga agagcgcact cgtcgagcgc gacgtcgatc
     9241 tcattgccga gctcgcgcgc gaggcgggcc tcagcctcaa tgtcagcctc gcgtggctcg
     9301 acgccgagct cgcgcggacc atcgagccgt gggctgcagc gccgcagcgc cggctcaagg
     9361 tgatcgagac atttgcgaaa gccggcgtgc ccgtcggcat catgatggcg ccgatcattc
     9421 ctgggttgaa cgacaaccaa atcgtgaagc tcctcgaggc cgcgcgcgaa gcgggtgcgc
     9481 agtgggctgg ctggtcgctg ctgcgtttac cgggcgcggt gaaggaagtg ttcgaagacc
     9541 gcctgcgcgt ctccctgccg ctcgttgcag accgcgtcat gaatcgagtg cgcgacacgc
     9601 gtggcggcaa caagctctac gacgcgacgt tcggcgtgcg cggccgtggc gtcggcgtct
     9661 acgcgcagac gattgcctcg ctgttcgaca cgacggccaa gcgcctcggc ttcggagaca
     9721 acgacgagcc ggatcctccg tcgagatttc gccggccgcc caagccgacg aatcagctcg
     9781 cgctgttctg accagtacgt cgtgctcgcg ttccgcgtga ttgcgacacc gctgacgcga
     9841 gggagtcgcc gagtcgccga gcacggtcgc gatcagctgc tcagcaccca gacgaggccg
     9901 gcgagcgcga gcaccacgat cgcggccgcg acatagaacg gcgcggtgct ggcgcgtcgc
     9961 tctgagctcg gggcgttcgc acgttgcgag ctgcgcaaga cagaggaccg ttcctcgccc
    10021 cgcgaggcgc tgttgtcgac ggaggcggtg cgcgcgcctc gctctgtgtg gtcgtcgctg
    10081 cgggactcgg ctgcagacgt cgccggccga tcgatcggcg gcggcaagtg tttctccgcc
    10141 gggtcgcgaa agatgaacgc gagcttgccg agctcgatgc gggagccatc gtgcagtgct
    10201 gtcgccgagt cggtgcggct ctcacgcggc gacggcgccg ctgtgatgcg cttgccgttg
    10261 atcttcgtgc cgttcttcga atcgagatcc gtgatgaacg tgccgtccca cgtgcggcga
    10321 atctcgacgt gtgcgcgaga gagatcgccg tcgtcgatga tccagtttgc ttcgtcgccg
    10381 cggccgatca cgagcgtcga ttcgggcgct gcgagcgggc gcttcgcacc ggcgagtggg
    10441 ccgctcgtga tctcgagcgt cggcgcggcg gcgctaccga ggagattgcg gagcagctcg
    10501 cgtgcgaggc tctcggtgcg ctgcggcggt gttggcgtag cgccgctcgg cgccggcccg
    10561 atcgtcaccc ggtagctcgc gatctggatg acgacaccct gccagacgac gccctctgca
    10621 acggtcgcgt cgccagggag ctcggcggcg gcggcttcgg agcccgtgtc gtcgtcggcg
    10681 gcggcggatt ttttagcggc atcgcgccat cgcaccggtg cgagcgcgac ccagcgtccg
    10741 ccggcatcga ccttgacatg ctcggacgtt gcggccgtct cgggcagtcg cacgcgcgcc
    10801 gtcagtgacg aaccgatcac gaagggcggc gcgagatgga tcggcggcaa cggcggcgag
    10861 ccgatctccg cgacgtcgat acgcaagagc gtgctcatgc gtcgtcacgc ctccgtcgcc
    10921 tcgacgcgca cgtacctacc ggcggtacct cccctgcagc gctcatcgtg cccgtcacgc
    10981 ggcgcgagct tactgcacgc gctgctgcgt ctcttgcgtc ttgtactggt agtagcgctc
    11041 gaggaccatc ttcagatact ggcgcgtgta cgtgtacggc gggacggcgt agccaaactt
    11101 cgcgagcgag ccgaggccgg cgtggtacgc ggcgatcgtg agcacgaggt ctccgtcgag
    11161 gcgattggcg agatagcgga gcaaccgcgt gcccgtcatg atgttcgtgc gcggatcgaa
    11221 gatgtcgccc tgcgcgccca tgtcgagttg gaccgccggg tggacctgca ttagcccttt
    11281 gcaatcgagg cccgagacaa cgtgcgggtc gaaatcgctc tccgtcttga tgacggcgcg
    11341 gatcagcggc acggggatgc gatagagctc cgacgcttca tagatgtact ggtcgtagcg
    11401 gtggaagcgt tccggtgagt ggtccgtggc gccgactttg tcgtagcgcg gcccgcgatc
    11461 gaccgctgcc ttgctgtctt tgggtggcgc ggagtcgagg acttttttcc acttgccgcc
    11521 gccgctgggc ttgaggttcg tgaagtgaac gacgccctcg tcgtccgtgt acgaataaat
    11581 atcggcgcgg gcgagcgtcc cgaacgcgag tagcagcatc aaagaggtca gcgcgcgcct
    11641 cacgtccgac gagcgtaccg cgcccccacc gccccggcaa aagctgtatg gtccgcccac
    11701 acatggccga gattctcgag accgactacc tcgtgatggg atcggggatc gcggggctca
    11761 atttcgcgtt gttggcggcc gagcatgggc gcgtcgtcgt cgtgacaaag aagcggccgg
    11821 ccgacacgaa cacgaactgg gcacagggcg gcgtcgcagc agtgctcggc cccgacgaca
    11881 gcttcgagag ccacatcgcc gacacgctga aggccggcga cgggctctgc gaccccagta
    11941 ccgtcgagat cgtcgtgcgg gacgcgccgc gcgaggtgca gcgtctgctc gacctcggcg
    12001 tgcggctcgc gcgtgacgag cgcgggaatc tcgacctcgg cctcgagggt gcgcattcga
    12061 agcaccgcgt cgtccactgg gaagacatca cgggccgcga gatccagcgc gctctaatcg
    12121 cagctgtcga gaaacatccg aacatcacga tgctcgacag ccatatcggc gtcgacctgc
    12181 tgtcgatggc gaagtacggc ggcgatccgg cgtgcttcgg cgcttacgtc ctcgacgtgc
    12241 gctccggcga ggtcaagacg atctgcgcgc gtgcgaccgt gctcgcgacc ggcggcaccg
    12301 gcaaggtcta catctatacg tcgaaccccg atgtcgcgac cggcgatggc gtggcgatgg
    12361 cgtaccgcat cggtgccgcg tgtggcgacc tcgagttcac gcagttccac ccgacggtgc
    12421 tctatcaccc gctcgcgggc agcttcctca tctcggaggc gttgcgcggc gaaggtggcg
    12481 tgctcaagaa cgcgcgcggt gaagagttca tggactacca ccccatgaag tcgctcgcgg
    12541 ctcgcgacat cgtcgcgcgt gcgatcgaca acgagctcaa gaagtccggc gcggactgcg
    12601 tctacctcga catgacgcat ctcgatccgg cgatggtcaa aggccggttc ccgaacatct
    12661 acgagcgctg cctctcgctc ggcatcgaca tcacgaagca aggcatcccc gtcgtcccgg
    12721 ccgctcacta tatgtgcggc ggcatcaaga cgaaccacga cggcgcgacg acgatccaag
    12781 gcctctacgc gatcggtgaa tgcgcgttca cgggtctgca cggcgccaat cgcctcgcgt
    12841 cgaactcgct gctcgagggc atggtcttct cgacgcgcgc ggccgctgcc gtccgcgaag
    12901 cgccgcggat taggccgtcg caggtggcgt catggtcgta cggcggcgcg accgactcca
    12961 acgacgcgat cgtcgtgtcg ctgaactggg aagagattcg ccggttcatg tggtcctacg
    13021 tcgggatcgt ccgcagcgac aagcgcctcg agcgcgcccg ccgccgcatc gagatccttc
    13081 gcgacgagat tcgcgagtac tacatcgact tcaaggtgac ggccgacttg gtcgagttgc
    13141 gcaacctcgc gctggtcgct cacctcatca tcgagtcggc ccgccgccgc aaagaatcgc
    13201 gcggtctgca ctacacgctg gactacccgg acaagcggcc cgaagcgctc cactccgaga
    13261 tccagctgac caacggcccc cgctcgtagc gcgcgcccgc gcggtgagct gccggctcgc
    13321 tacggcggag tggccgctgc gcggacagaa aaacttaccc cggtacgttc gaaaacttac
    13381 cccggtaggt ttgcccgtcg gcacttcaca caacacaggg tcaacgcgcg ttcacacggc
    13441 gcggggaaat ggcacgtggg aatggccact tgcgggcgtt gaatgtcgta accgcgggcg
    13501 cgtccatctg gcatctgcct cgcaatactg ctgggcataa ccttcgctgc cggatcttga
    13561 cggacggcca cgcagcgcgg ggggccaccg aagcatggtg cttcggtggc tttgtctttt
    13621 cggcgacgct acattcacgt gagtgcagcg gctcgccctc gttctcgcgc tcgctctagc
    13681 tggtggcggc gccgccgccg gctgtcatcg agcgcgcgga ccggcgtcgc ccgcgagcga
    13741 cggcgttcgc gagtatctga aggcgctgcg gtcgaatgac ccgcacgacg cgtatgacct
    13801 gctcgcgtcc gacgtgcgca agaagatcag ctacagcgag ttcgcgctgc agtggaagca
    13861 gagcgaggcc gagcgcaagt ggcaggcgaa ggtgctcgag gactcgctca aggggaaccc
    13921 ggacgtcggc gagcgagcgc tgatctcgtt tagcgacggc aagctcgtgc agctcgagcg
    13981 cgagggcaag acctggcggc tggagtccga gctggtctcg cggtcgcgcg cgaagcgtcc
    14041 acgcgatgcg attcgtatgt tcgccgatgc gatcgcggcg cgcgacgtgg gtgccgcgct
    14101 caacgtgctg acgcagcgcc gccgtgaggg cctgtcgaag cagatcgaag gcttcatctc
    14161 gggcatcgcg aagcacgcga gcggtccgct cgacgagcag gccgaccgag ccgagctgcg
    14221 ctgggatgaa aatggaattc gatatcgcat cgtccttcgc aaggaagacg atgagtggcg
    14281 cgtcgacgat atctacatcc ggcccgcgcc caaagacgaa gacacgacca agcccggcga
    14341 tatcgaaggc ctgcccggcg acgacttcta aggttcgctc gtcgcgatcc tcgttcgcgc
    14401 cccgcgcact cgcggttgac gcctgcttca gcgcaactca cgggcgcgcc cgtcgttcgc
    14461 gaacgcgcgc actagctcac ggacaggcga cgccgttcgc gagcagctgc cccgttgtca
    14521 ccaccgccgc gtgcgccgct tttgcgtcgt agtgcttgct cacggcgacg gccgccagcg
    14581 cccactcgtc tccacgcacg aagatcgcga tggccatcgg gctcgagctc tcggcggtga
    14641 ggcaaaccgt catgcgatcg ttcgactgac tgcagtggat ggggctcgcg aatgaggcgg
    14701 acgtctgcgc gagcgcagtg gtggcgcgct ccccgcacac gacctgcgtc gtccccgcat
    14761 cgatatcgcc gacgacgatc gggccacgcg cgagctcgcg aagcaccggc aatgcattgt
    14821 cgcctagctc gcgctcgatg cgctcacgcg accacgggct tcgaggagcg acggcgtgcg
    14881 aggcggcgtg cgacgtaccg agcgtctcgt gcgctgtcgg cggcggcgtt gtcgatccgc
    14941 agcccaccgc gagcgctagg atcgcgatgc gcgtcactcg tcgtcgaccg gcgagctcgg
    15001 cggcgccgtg aacggcgtct cggagagctg cgccatcgcc gtcgcgagat ttgccacctc
    15061 caccgacggc ggcgcgttgc gcttcagcat gaacgtgcac gtcgcgcagg acgtgacgac
    15121 catgccgccg ccggatgcct gcacttcggc gagccggcgc ttcgccattt ggtcggcgac
    15181 cgttggcatc gtcttcggca ggaggccacc accgccgcag cattcggtgt cggcgcggtt
    15241 ccacgagaac tcgcgaagct cggcgagccc tgcgagcgcg cgacgcggcg cttcgacgac
    15301 gttgttgtag cgcgcgttgt agcaagggtc gtggtagtag acgctcttgc gattgagcga
    15361 gcacgcgaga ttgttcgcgg cgggcgcgag cagatcggcg agcgagacga cctcggtgcg
    15421 cacggagacg ccttcggccg cgtactgcgc gcgcacggcg tagagacatg cgctgcagtt
    15481 catcgcgacc ttgcgaaagc cgcgcattgc cgacgcgacg cgccccgcgt gccagcgaaa
    15541 tagatcgaca tgacccgcgg cgagcagtgg atagcccgcg catgcttgcg gtgccgagac
    15601 gatcttgacg gtctcgagcc cgacgcgctc gaacagcgcc atcgcagctg cgatatcggc
    15661 cgcgcctttg tcgattgcat cgcagcccgg ccagaagccg acgtcacctt ctgtcgcgag
    15721 gtgctcggag aagccggcgg cgagctgcgc ggagagccgt tgctcgcgcg cgcggaaccg
    15781 ttcgctgtag ccttcgagcg cgggatgctg cgtgccgtgc tcgacggcgg cggcgcgtcc
    15841 cgtgaagagc acgagccccg gctcgttgcc atgatcgcaa tacatcgtgc agtgacggca
    15901 gcccgtgcat gcgtacagcg gatcggtcga ctcttcggtc caccgactgc ggccgaggcg
    15961 cagctggttc atccgatcca ttttgacttg cgggatgtac gtctcgcggc cgctcgccgt
    16021 cgacaccggg cacgcgtgcc ggcacatctt cgggcagtac gtgcagtagt cgagctggcg
    16081 cgcgacgctt cccgcgctga acggctcgtc tgcgtctttg tgggccatcg aggtgaccca
    16141 ctatacggct aattgaggcc gtcgactcgt tcgcgcagct gcgcgaggtc gcgtgcgatc
    16201 cgcggattcg atggctcgag cacggcgagg cgttcgagca cgtcgagcga gcgaggcaga
    16261 tcgccgttgc ggccgtagat gcctgcgaga ttgacgagca tgcgcgcgac gatttgtcgc
    16321 ttcgacgccg cagcgatcac cgacggctcg agaatcgcgt tcggcgtcgt cgtcttgcgg
    16381 aggagctctt cgagatccgc cggcgtgagc gtgcggccat gcgtatacgg atcgaggaac
    16441 agccacgcat cttcgatcgc gacgcgcacg aggaagtgcc ccgggaagtt cacgccctgc
    16501 gcgagcacac ccacgcgacg cgcaacttcg agatagacga ccgagagcga gatcggaatg
    16561 ccttggcggc gatcgatgac ttcacacagg aagctgttgc gcggatcgta gtagtcgctg
    16621 ttgttgcccg agaagccgag gcggtcgaac aggtgctcgc tgatcgcgcg tgcgcgaccg
    16681 attccatttg gcgcgcgtgc aacatcgggc gcgcaacgag cagaaatctc gtcgagcatc
    16741 tcccggtacc tctcgagatc gcgctctggg taatcccacg cgccgatgag cagcgcggcg
    16801 tgatccagcg aaaagtcgtc ccgagccacg acgtcgctga acctagccag acccgggctt
    16861 ggttccatag ctctcgagtt taccattcga cgctgttcgc gcggattgtt tcctttggct
    16921 atgcgaagat gccccttcaa tggctgagga gccgcgccct gggctgatcg tcggaggacg
    16981 gtacgagctc atcgaagttg ctggccgcgg tggcatggcg gatgtatggc gcggtcgcgt
    17041 tcgcggcgac tggggattta cccgcgacgt cgccatcaag cagatgcacc agaatctcgc
    17101 atcgcagccg ccgtatgtag cgatgttcgt ggaagaggcg cggctcggct cgatgctgca
    17161 ctctccgaat gttgcggagg tccacgattt cgtacacgac cgcggcaact actacatggt
    17221 gctcgagtgg atcgacggcg tcgatctcgg cacgtggatt cgctggcacg catcgcgtaa
    17281 cgagcagacg cggtgggagc tcgtcgcagc cgtcgccgtc ggaattcttc ggggactcgc
    17341 cgccgcgcac gaacgaacgg cgccggatgg cacgccgatg cccgtcgttc atcgcgatgt
    17401 gtcgccgcac aacgtgctgc tgacgaatcg cggtctcgtg aagttgatcg atttcggtct
    17461 cgcgctcgcg cccgatcgcc cgcaggagac caccgagccc ggcatcgtga agggcaagat
    17521 gagctacctc gcgccggaga tcgtcggcgg caagcggccg atggccgaga gcgatcagtt
    17581 cgcgtgcggc tcggtgctgt gggaagcact cgtcggtcgc aagctattcg acggcgcgac
    17641 ggactacgag gtgtattgcc gcctgcgcga ctgcatggtg cagccgctgc gcccacttcg
    17701 tccggacgtg ccggcggcat tcatccagct gatcaatcgc gcgctgtcgg cgaatcctga
    17761 cgcgcggttt cctaccgtgc gcgagatggc gcggcagatc ggcaccacgc tcaagaaggt
    17821 gcagctgcgc aaggatctcc actcggtgct cagcaagagc gtcgcggatg ccaagcaagg
    17881 catgggcctc ggtccgcgca cgggcgatcc atcgtcggct acgccggtcg cgcagatcgt
    17941 cggcgacgat acgccgcgga tcgagctggc accgcgcatc gacacaccgg tggaaggcgc
    18001 atcggtcggc gcgctcgaga agctgcgcgg gctgcgtcac cgcttgccgt cgttcttcgg
    18061 acgaaagcgc ggttaacgcg tgcgccgcct cgctctgctg ctgatattcg cagcgtgcgg
    18121 cgataacgcg gcgccgccgt tcgtgccgct gcaatcgggc aacgcgctca tcacactcga
    18181 cgatggtgcg ctcgtgctgt cgcgcgacgg cgtcgagaag accaggctcg caccgagcgc
    18241 gttccagatc ggcaccgtcg acgatctcga tagtggcgcg agcttcgatc cctattggct
    18301 gtgggtcgac aacgcgcccg agccgcccga aggcctcgcg tggcatgcag cctcgagctt
    18361 gcgtgtcgag cgctcggata cgcagggctt cgcgctctcg ctcgacgacg tcggcaacgt
    18421 cgagtgcgtg ccgtcgacgc cgggcagctt ccggctcgtg ttctcgtcgt cgcgcacccg
    18481 cgtcgcgttc ctgcgcgtgc agccggcggt gaacgacacc gagcgcttct atggtctcgg
    18541 tgaatggggc gacgctgtcg agcatcgcgg caagctgagg ccgatgcagc tcgaggtcga
    18601 cacgacgagc gaatcggcga ccaacgaagc gcacgtgccg gttccactcg cgatctcgac
    18661 gagcgcgtgg ggcgtgttcg cgcagagcaa gcggcccggc gcattcgacg tcgcgcgcat
    18721 ggatccggcg aagctcgaca tcgcattcgg caccgccgac gacagcgcgc gtggcctcac
    18781 gttccatctg ttcactgctc cggacggtga accgcttggc gttctgcagc cgtacttcga
    18841 ggtgacgggc tatccggcgc ttcccgcgta ttgggcatac gggccgctgc tgtggcgcga
    18901 cgagacgacg ggtcaggtgc agacgctcga cgacatccag cagatccgca cgcgtgatct
    18961 tccgacgagc ggtatctggt tcgacaggcc gtacgcatct ggcgtgcaga ccttcgactg
    19021 ggatccagca aagttcgcgg cgcccgccga catgctgcac gcgctccacg acgcgggact
    19081 gcgctacgcg atctggcaag cgccgtacgt cgcgcccgcc aacaacgatc aggatccggc
    19141 gcccgatcaa aacgcgtacg cgactgcgca tcacttcttc ccgccgacgt cgggcattca
    19201 agcgaatcag tggagcaagc cgatcgactt cacgaatccc gaggcgtacg cgtggtggca
    19261 ggcgaatctg aagaagtaca ccgacccgct ctcgctcggc ggctggggcg tcgaaggctt
    19321 caagctcgat tacggcgagg acatcgtgct cggcgcgctc ggtcagcgcg tgccgtgggt
    19381 attcgccgat ggcagcgacg aacgcacgat gcatgtcgac tacacgctcc tctatcacgg
    19441 catctatcgc gacatgctcg acgcggatgg tggcttcctg ctgacgcgca ccggccgctg
    19501 gggcgatcaa acgcgcggca tgatcgtgtg gcccggcgat ctccaagcag acctctcgaa
    19561 gttcggcgat tcaaagccgg ggtcgtcgtc caagaccgtc ggcggtctgc ccacggcgct
    19621 cgcattcggg atcggcctct cggcgtcggg ctttccgttc tacgcgtcgg acactggcgg
    19681 ctaccgcagc tcgccgccga acaacgagac gtggctgcgg tgggtcgaag ccaacgccgt
    19741 gtggagcgcg atgcaggtcg gcgactcgtc gagccagatg ccctgggagt acacgacagc
    19801 gaacggccgc tcggatgcat cgctcgacac gtatcgcgag tacgcgcggt tgcacctgcg
    19861 gctgttcccg tacgtgtggt cgctcgcgca gcagatgcgt gcgacgggct acccgatcgt
    19921 gcggcctctc ggtctcgcgt atccgggcat gccggagcat ccagccgatc aatatctcgt
    19981 cggtgatgcg ctgctcgtcg cgccggtgat caccgcgggc gccaccgcgc gcacggcatg
    20041 gtttccgcgc ggcacgtgga tccagtggtt caccggcgaa gaattcacgg tcgccgcggt
    20101 cgagcagcgt tcggtagccg ccgccctcga cgagctgccg ctgtacatcc aacgcggcgc
    20161 catcgtgccg atgctgcgcg acacgatcga cacactcgct ccggccacgg tgtcgggcat
    20221 cgagtcgtac gcgaatgatc caggcctgct tgtcgtgcgc atcgcgccgg gaccccacag
    20281 cgcgttcacg ctgttcgacg aaacgaaggt cgagcagcag ctcgaagcgg gagtcacgac
    20341 ggtcgattac acgccgggca agacgttcgt cgctggcgcg ctgctcgagt cgatcgcgac
    20401 cgatgcgccc gccgccgtga ccacggaatc gggtccacta acgcaacgcg caagctacgc
    20461 cgccctgcag gccgcggccg acggttggtt ctacgacgcg acggccacgg gcggaaccct
    20521 gtggatcaag ctcgccggcg ccgccaccgt tacc
//
LOCUS       DATLBZ010000561        13434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_2229263, whole genome shotgun sequence.
ACCESSION   DATLBZ010000561 DATLBZ010000000
VERSION     DATLBZ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229263"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VMZ53_28030"
     CDS             complement(<1..236)
                     /locus_tag="VMZ53_28030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32394.1"
                     /translation="MRRALIVIGVVTLAAVASAEQVARKPPPPKPTCAEIEKREAAVK
                     KREDAVAKREADVTKREVELAEKEKRDMERVRRE"
     gene            complement(221..1462)
                     /locus_tag="VMZ53_28035"
     CDS             complement(221..1462)
                     /locus_tag="VMZ53_28035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32395.1"
                     /translation="MLLLLLSLLLLLDLLRCLLLLLSALLLLLLLVSGDDLRRLLLLL
                     LALLLYLPDPRREIDSRLRDDRVSRIAAARSIGALLFLPLALVFFDFSDRVFARVVEL
                     FSPRKDSVDLRSGRRVRRRECDHRAAVSFVVEELATFAHRCESSLPSQLAHLGPRVVH
                     AVSNVLTAKARTMLTRQQYALLVVGLGSKPRCHGLLAKARRFRSVVVRAIKREREHLL
                     ERPAGFTVRIRPPDVRGHHAERVLVELDRLVREHVEAHLPVRVISYLIGHLVVLDPEH
                     AWIDALRWSRHELDLARASDERFATHAIGHPRLPGIAAAPEPVRLVALHIGRAANRGI
                     HAKPRRVTRLELIERRDRLSAPRAALADVFADLARFERISRGVVCRRMMTGDERYYRN
                     HNNTHHAHHNRADILVACDEL"
     gene            1514..1945
                     /locus_tag="VMZ53_28040"
     CDS             1514..1945
                     /locus_tag="VMZ53_28040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32396.1"
                     /translation="MHVTQTNPFPGLVTHYDSIRRRAAACPKNADNIGKQGRADWLAC
                     MNGQLWLETERMTNQSAFSERRGRVHLPTDTLSCVPFEKDLQSYCDTMTRDNCIKVLD
                     YTVKLLRDCDAMATPLFTAEDQANNDEAQKRHDDECRRRYP"
     gene            complement(1947..2657)
                     /locus_tag="VMZ53_28045"
     CDS             complement(1947..2657)
                     /locus_tag="VMZ53_28045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32397.1"
                     /translation="MLRSLLVVVCLRGIAFAETPPAPHIAPSPTDNAPPTMPASSGTP
                     KGWAPLPGSAPTQPRVAITPAVPPTPASAPPAAASSSAMATAPAPAPAPVATSTRGAT
                     AAVGITARPFEPHHGLALDVRLAAGFTSGTYTSLGLAQVTVGVGAWIAPHIAVVARGE
                     IGVLQTVGGEVRGELGPRGVIGGGLGYVHLDDKSGGRLHAHAAYRFTPNLGVDASVGV
                     DLLNHTAADLTLGVIYLR"
     gene            complement(2670..3107)
                     /locus_tag="VMZ53_28050"
     CDS             complement(2670..3107)
                     /locus_tag="VMZ53_28050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32398.1"
                     /translation="MKLDLARLGFVIENEWYWKITENAQTFDVASAIRDFVAPLARYA
                     KDKLAITDIAADLAEVRFALKGEPVAIKVEVGLHDRVAVNHFVGDLNRELSRLHLAFA
                     LVVPRRYELRGVLLTEKELASLAGDQALLVPSARPSWRSIPTT"
     gene            complement(3132..3479)
                     /locus_tag="VMZ53_28055"
     CDS             complement(3132..3479)
                     /locus_tag="VMZ53_28055"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUS32399.1"
                     /translation="MKVVDKPWGNELWWAVTDRYVGKILHIKQGEALSLQYHRVKDET
                     IMVLTGRLALEYFADGEQPQTRELGPREPFHITPGTRHRMVALADTDVLEVSTTELDD
                     VVRLEDRYGRAGT"
     gene            complement(3484..4218)
                     /locus_tag="VMZ53_28060"
     CDS             complement(3484..4218)
                     /locus_tag="VMZ53_28060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32400.1"
                     /translation="MMLKASKLGVLVVGAAALAGCSKKKAPTTPKNEIGMEVPKVDPT
                     LCETVGKNVVTYDLNHDNRPDVWRLYRTEDEGGTKIEFMTCKQVDFDHDGRKDWVVGY
                     NRKGNPLFEKADFDYDGKFDMSAVFDPKTAKVAEVERDIDRNGSFDVKEIYDTSGTLQ
                     SIRKDRNGDSIPDEWEQYKENILIAILYDDDFDGKVDRRDEIPGATPKVEMPTTMDTS
                     QASGAISPDKAAPPSSSAPGNTPKKK"
     gene            4302..4487
                     /locus_tag="VMZ53_28065"
     CDS             4302..4487
                     /locus_tag="VMZ53_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HUS32401.1"
                     /translation="MARKKISTTVYITPEQDARLKALHGRTKVPVAEYIRQGIDLVLE
                     KYRDQLPGQVDLHLGSR"
     gene            4504..6306
                     /gene="lepA"
                     /locus_tag="VMZ53_28070"
     CDS             4504..6306
                     /gene="lepA"
                     /locus_tag="VMZ53_28070"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HUS32402.1"
                     /translation="MSFPPERIRNFSIIAHIDHGKTTLADRILDECGAVTAREAQAQM
                     LDSMDLERERGITIKAQSVQLHFKSKDGQTYQFHLIDTPGHVDFHYEVSRSLAACEGA
                     LLVVDAAQGVQAQTLANVYVALDNNLEIIPVLNKIDLPVARPDEIKQQIEDTIGLDAK
                     NACLVSAKSGIGVRDMLEAVVERIPPPKADREGPLSALIFDSWFDQFRGVVVLVRVIS
                     GTIKKGTKLKFMGTEVTYECTMIAVRRPATFEIETLEAGQVGILAASIKDIAHARVGD
                     TVTDADNPVDAPLPGFKTVKPMVFAGLFPVDAADYQDLKDALGKLVLNDASVTYEPET
                     SSALGFGFRTGFLGLLHMEIIQERLEREFNLNLITTAPSVRYRVKMNGGEIVELDNPA
                     KVPDQGKYDAIEEPIILATVHLTQEYVGPIMQLCQERRGVQQGLHYDPGGRVRVVYEM
                     PLAEVVFGFYDRLKTMSRGYASLDYEPAGYREADLIRLDVLVNGERVDALSNIAHRDS
                     AYHRGVELCSKMKDVVPRQMFDVAIQAAIGSRVISRTTVKAARKDVTAKCYGGDITRK
                     KKLLEKQKEGKKRMKAVGSVELPQEAFLTILKVD"
     gene            6310..7419
                     /gene="lepB"
                     /locus_tag="VMZ53_28075"
     CDS             6310..7419
                     /gene="lepB"
                     /locus_tag="VMZ53_28075"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HUS32403.1"
                     /translation="MRASRLDRRIRKEAWTLVRDARAALSLKKGLRGKGGDLATVTGE
                     VEKGLVDKDMKRVRDNLPVLDALVDELVKRPPKSTTRDYVESIGAAILIALALRAFVI
                     EAFKIPSSSMYPTLEIGDHIFVNKFIYGVRIPYTNIKFFELRGPHRGEVIVFQYPCDM
                     DRDYIKRVIALEGDTVEVRCNIVYVNGKAIPSKMVQDGDHCSYKDYDEREDRWFMRKC
                     SRYQETVDGEPYDTFHDDERPKRELAIARGEQINDQRDFPSAGSRLIPSCVQAEEPGQ
                     SAENVPQVKGTLIEIKPESVATACEQQLQYKVPAGHVFVMGDNRNNSNDSRIWGSVPI
                     ENIKGKALFIWLSYEHWGPLDWGGIRWYRIGNFVR"
     gene            complement(7438..8019)
                     /locus_tag="VMZ53_28080"
     CDS             complement(7438..8019)
                     /locus_tag="VMZ53_28080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32404.1"
                     /translation="MKSLFLFALSLVLAMPIAADAAPKKKYHFELTKVLLKAEVKGDA
                     GKTAQSRVEAVFKKAMAEHPQLVADITGAPDPAKDNGGPYRKWLTKKGINGAYLVTVE
                     ITSASEELVPLEDKKNAQRLVVSVGVHVLGETIPGRTMGFTGDGAATVKQELGMKVRE
                     KDRELTWDDAAKVAIDDALKTCFAKLSLPQKKQ"
     gene            8327..10786
                     /locus_tag="VMZ53_28085"
     CDS             8327..10786
                     /locus_tag="VMZ53_28085"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HUS32405.1"
                     /translation="MFTIIIQEKGGEQRRMVFNKPEVTIGRVQGNDIVLPKGNVSKRH
                     ARIVLKDGKFIIVDLKSTNGTYVNGRKITSPLVVKDSDKIYIGDFIVGVDEAAAEGDG
                     PSETTTSPPGSDRPIDPRPPAPTTASPQPMGGMPGMGMGGGMGGMGGMGGPPEPPPPA
                     APPAPSMPPPMGARGPGGGPPRPPGGPPRDLGGPPRDMDGPMGGGPMGGGPMGGGPMG
                     GGPMGGPMGGGPMGGPMGGGPMGGPPREPPGPRGLGGPPREPPGPSRDMPPIAPPPAN
                     EARTRPPVRGAGGTMPPPMAPIPSPTSAPPPPDPHFPPPAAMPPPGPMGGAPMGNPMA
                     MAPPVAPPTAAPMPVAVAAPSIAAAPQGTPAQVVPLQKDKSARTVSAVGTQPSKKITG
                     RPLAPAAKRGVQLEPLDSKIVKMLDLQANILERLRAKLDLDRVPLERLHEDELWQRAE
                     KATIDLVETLETSGELPKYIDQEALIKGTLNEALALGPLEDLFQDDSIDEILIDRRDR
                     VVVGKGGQLRGSGKAFSSDEMLERVVKRLVFEAGSVIDESHPVVDLRMRDGTRVTAAV
                     APVAARGACLVLKKPPASTPTLADLVSQGVLSNGMAEFLTTCITARRNVLVCGGPGSG
                     KTTLVGALAGAAPAGERIVSVEDVAELAIGRDEWIQLETRPTNGKGNDVDLGALLDTA
                     MRLMPDRLVVGDVRGREAMPLVNALNSSVDGAIVGMSGEGTSAALNRLATLARATSAL
                     GADWTAIRELVGTAFEIVVHVTRGTDGTIKVSSIEELSGVSDVAFETEQVFCHKDGGF
                     AASGKVPRFYSELGADQAVFR"
     gene            complement(10793..11560)
                     /locus_tag="VMZ53_28090"
     CDS             complement(10793..11560)
                     /locus_tag="VMZ53_28090"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUS32406.1"
                     /translation="MMATLTMATDETNADDKLTIDELAAKSRVPSRTIRFYQSRDALM
                     GPTIRGRVAYYGDQHLERLKLIAQLQDRGLRIDAIGDLMKRIDRGEVDLAEWLGVEEQ
                     MQTPWAGDHARTVTEAELYELAGNRRPGLLADLGRAKIIERKGDVFLVGSPALLSIAM
                     KLEGVGIGLEVAAKASGILRKHLSRAVDELVELFVGEVKEGHVSIAESGKLMETFRAV
                     GVDSVRVLFARQMESALRKLLASGKLASVAARTRKKR"
     gene            complement(11557..12657)
                     /locus_tag="VMZ53_28095"
     CDS             complement(11557..12657)
                     /locus_tag="VMZ53_28095"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HUS32407.1"
                     /translation="MTHSLPVALDDDAIEAFGRELDAIQREVKASLGESDRRYIKRAI
                     KVQRGLAFGGRMMIFASLPLIPAPPLFIGVIGAGTIALGLSKILENMEIGHNVLHGQW
                     DWMQDPEIHSSTWEWDNVCPADQWKHSHNVVHHTWTNVLGKDRDVGYEILRVTEMQRW
                     KPAHLAQPVYNALLAMLFQWGVAMHDVDLGAVKRGQLAEARGKLAGIGKKASRQLLKD
                     YVLWPLLAGPFFLPVVIANAIANLMRNLWSYAIIFCGHFPDGAQVFTEQQIEGETRGR
                     WYLRQLLGSCNIEGSPLFHVLSGNLSHQIEHHLFPDMPSNRYPEIAPRVEAICQRYGL
                     PYNKGSFARQLGTTMRTIFRLALPTKRAVLAA"
     gene            complement(12670..>13434)
                     /locus_tag="VMZ53_28100"
     CDS             complement(12670..>13434)
                     /locus_tag="VMZ53_28100"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HUS32408.1"
                     /translation="QGKVSNALVRDVCVGDYLSIGLPDGEFVLPDALPDRIGFVTAGS
                     GITPVMSMLRSLDAGGELPDIAHVHVAKTAHGVIFGDELRALALKHPRYRLTTVLTVA
                     DPRRLCSERLHALMPDWRSRETWACGPQSLLDTLTACFAGHHMATALHVERFAAAFVA
                     VPSGGSSGDGGGRVRFSTTNREARSDGRTPLLRIAEEAGVNAPHGCRMGICHSCDTTL
                     VSGCVRDLRTGERITEPGTRVQTCVCAAAGDVELAL"
BASE COUNT         2414 a         4299 c         4391 g         2330 t
ORIGIN      
        1 tgttcccgac ggactcgctc catatcgcgc ttctctttct cggctagctc cacctcgcgc
       61 ttcgtcacat ccgcttcgcg cttcgcgacg gcatcttcgc gcttcttgac cgcagcctcg
      121 cgcttctcga tctccgcgca cgttggcttc ggaggcggtg gcttgcgcgc gacctgctcc
      181 gcgctcgcga ctgcagccaa cgtcaccacc cctatcacga tcagagctcg tcgcatgcaa
      241 ccaggatatc agctcggtta tgatgcgcgt gatgcgtgtt gttgtggttg cggtagtagc
      301 gctcgtcgcc tgtcatcatc cttctgcata caaccccgcg cgagatgcgt tcgaagcggg
      361 caagatcagc gaagacgtct gcaagagcgg cacgcggcgc ggacaaccgg tctctccgct
      421 cgatcagttc gaggcgtgtt acgcggcggg gcttggcgtg tatgccgcgg tttgcagcgc
      481 gaccgatatg gagtgcaaca agccgcactg gctctggggc agcggcgata ccgggcaagc
      541 gcggatggcc gatcgcatgc gtcgcgaacc gttcatccga cgcgcgtgcg agatcgagct
      601 cgtgacggga ccaacgaagg gcgtcgatcc atgcgtgttc tggatcgaga actacaaggt
      661 ggccgatgag gtagctgatc acgcgtactg gaaggtgtgc ttcgacgtgc tctcgcacga
      721 ggcggtccag ctcgacaagt acgcgttcgg catgatgacc gcgcacgtca ggcggccgaa
      781 tgcgaacggt gaaaccggcg ggccgttcaa gaaggtgttc tcgttcgcgc ttgatcgcgc
      841 ggactactac cgaccggaaa cgacgtgctt tcgcaagcag accgtgacat cgcggcttgc
      901 taccaagacc gaccaccaaa agcgcgtact gctggcgtgt cagcatggtt ctcgccttgg
      961 ctgtcaggac gttgctgacg gcgtgtacga ctcgagggcc gagatgcgcg agctgggacg
     1021 gcaagctgga ttcgcagcga tgagcgaacg ttgcgagctc ttctacaacg aagctgactg
     1081 cagcgcgatg atcgcattcg cgcctgcgga cgcgacgtcc gctgcggagg tcgaccgaat
     1141 ccttgcgcgg ggaaaagagc tcgacgacgc gcgcgaacac acgatcgctg aagtcgaaga
     1201 acacgagcgc cagtggcaag aagaggagcg cgccgatcga gcgcgccgcc gcgatgcgcg
     1261 aaacgcgatc atcgcgcagg cggctgtcga tctctcgacg gggatcgggc aagtacagca
     1321 gcaacgccag caggagcagc agcaagcggc gcaaatcgtc gccgctcacc agcagcaaca
     1381 gcagcaacag cgcactcagc agcaacagca ggcaccgcag cagatccaac agcagcagca
     1441 agctcagcaa cagcagcagc aagctcagca acagcaggca cgtgagcagt gcctcgccca
     1501 aaaggtcacc ccgatgcacg taacgcagac gaacccgttt ccaggactcg tcacgcacta
     1561 cgactcgatt cgcaggcgcg ccgctgcgtg ccccaagaac gccgacaaca tcggcaagca
     1621 ggggcgtgcc gactggctcg catgcatgaa cgggcagctg tggctcgaga ccgagcgcat
     1681 gaccaaccag agcgcgttct cagagcgcag gggccgcgtg cacctcccca ccgacacgtt
     1741 gagctgcgtt ccgttcgaga aggatctgca atcctattgc gacacgatga ctcgagataa
     1801 ctgcatcaag gtgctggact acaccgtgaa gctcctgcgc gattgcgatg cgatggcgac
     1861 gccactcttt actgccgaag atcaggcgaa caacgacgaa gcgcagaagc gccacgacga
     1921 cgagtgtcgc cgccgatatc cctgactcag cgcaggtaga tcacgccgag cgtgagatcc
     1981 gcagcggtgt gattcagcag atcgacgcca acgctggcat cgacgccgag gttcggcgtg
     2041 aatcgatacg ccgcgtgtgc gtggagtcga ccgccgctct tgtcgtcgag gtgcacgtac
     2101 cccaggccgc cgccgatcac gccgcgtggc ccgagctccc cgcggacctc accgccgact
     2161 gtctggagca cgccaatctc gccgcgcgct acgaccgcga tgtgcggcgc gatccatgcg
     2221 cccacgccga ccgtgacctg tgcaaggccc aggctcgtat aggtgcccga tgtgaagcct
     2281 gccgcgaggc gaacgtcgag cgcgagcccg tgatgcggct cgaagggacg cgcggtgatt
     2341 cccacggcgg cggtcgcgcc gcgcgtcgat gtcgcgacgg gagcaggagc aggagcgggc
     2401 gccgttgcca tcgccgacga tgacgcggca gcaggaggag cagaagcagg agtaggagga
     2461 actgcaggcg tgatggcaac ccgcggttgc gttggcgcag acccggggag tggcgcccag
     2521 cctttcggcg tgccgctcga cgccggcatc gtcggtggag cgttgtcggt gggactcggc
     2581 gcgatgtgtg gtgccggcgg cgtctcggcg aacgcaatac cgcgcaggca cacgaccacg
     2641 agcaacgacc gcagcatgag cccagcgtat caggtcgtcg ggatcgagcg ccacgatgga
     2701 cgtgcggacg gcacgagcag ggcctgatcg ccggcgagcg acgccagctc tttctcggtg
     2761 agcagcacgc cgcgcagctc gtagcgacga ggcacgacga gggcgaatgc gagatggagg
     2821 cgagagagct cgcgattgag atcgccgacg aagtggttca ccgcgacgcg gtcgtggagg
     2881 ccgacctcga ccttgatcgc gaccggctcg cccttcagcg cgaagcgcac ttcagcgagg
     2941 tcggcggcga tgtcggtgat cgcgagcttg tctttggcgt agcgagccag cggcgcgacg
     3001 aagtcgcgga tcgcggaagc gacgtcgaac gtctgggcgt tttccgtgat cttccagtac
     3061 cactcgtttt cgatcacgaa gccgagacgc gcgagatcga gcttcactcg tgcaggctac
     3121 acgggagtat tttaggtgcc agcgcggccg tagcgatctt cgaggcggac gacgtcgtcg
     3181 agctcggtgg tcgagacttc gaggacgtcg gtgtcggcga gcgcgaccat gcggtggcgc
     3241 gtgcccggcg taatgtggaa tggctcgcgt gggccgagct cgcgcgtctg tggttgttcg
     3301 ccgtcggcga agtactcgag cgcgaggcgg ccggtgagga ccatgatcgt ctcgtctttg
     3361 acgcggtggt attgcagcga gagcgcctcg ccttgcttga tgtggaggat ttttccgacg
     3421 tagcggtcgg tcaccgccca ccacagctcg ttgccccagg gtttgtcgac cactttcacc
     3481 gagctacttc ttcttcggcg tgttgccggg agccgacgag gacggtggcg ctgccttgtc
     3541 gggcgagatg gcgccggagg cttgcgaggt atccatcgtc gtcggcatct cgactttggg
     3601 cgtcgcgccc ggaatctcgt cgcggcgatc gactttgccg tcgaagtcgt cgtcgtagag
     3661 gatcgcgatc aggatgttct ctttgtactg ctcccactcg tcggggatgc tgtcgccgtt
     3721 gcggtccttg cggatcgact gcagcgtgcc cgaggtgtcg tagatctctt tgacgtcgaa
     3781 cgagccgttg cgatcgatgt cgcgctcgac ctcggcgacc ttcgccgttt tcggatcgaa
     3841 gaccgcggac atgtcgaact tgccgtcgta gtcgaagtcg gctttttcga agagcgggtt
     3901 gcccttgcgg ttgtagccga cgacccagtc cttgcgaccg tcgtggtcga agtcgacctg
     3961 cttgcaggtc atgaactcga tcttggtgcc gccttcgtct tccgttcggt agagacgcca
     4021 gacgtcgggg cggttgtcgt ggttgaggtc gtaggtgacg acgttcttgc cgacggtctc
     4081 gcacagggtc gggtcgacct tgggcacttc catgccgatc tcgttcttcg gcgtggtggg
     4141 tgccttcttc ttcgagcagc ctgcgagcgc cgctgcgccg acgaccaaca cgccgagttt
     4201 cgaggccttt agcatcaccg gcgaagccta atcggggagc ttcggacccg caagcgcacc
     4261 gccttgacca tgtggatatg gtcacgtagg gtcacggctc cttggcccga aagaagatct
     4321 ctacgaccgt ctacatcacg ccggagcaag acgcgcggct caaggcgctg catgggcgga
     4381 ccaaggtgcc ggtggccgag tacatccggc agggcatcga tctggtcctc gagaagtatc
     4441 gcgatcagct ccccgggcag gtggacctcc acctcgggtc acgctaacta ctgagccccc
     4501 gcgatgtcgt ttcctcccga gcggatccgt aatttctcga tcatcgcgca tatcgatcac
     4561 ggcaagacca cgctcgccga tcgcatcctc gatgagtgtg gcgccgtcac cgcgcgtgaa
     4621 gcgcaggcgc agatgctcga ctccatggat ctcgagcgcg agcgcggcat cacgatcaag
     4681 gcgcagtccg tccagctcca cttcaagtcg aaggacggcc agacgtatca gttccacttg
     4741 atcgacacgc ccggccacgt cgactttcac tacgaagtct cgcgctcgct cgctgcttgc
     4801 gaaggggcgt tgctcgtggt cgatgccgct caaggcgtgc aggcgcagac gctcgcgaac
     4861 gtctacgtcg cgctcgataa caacctcgag atcattcccg tcctgaacaa gatcgatctg
     4921 cccgtcgcgc gtcccgacga gatcaagcag cagatcgagg acacgatcgg tctcgacgcg
     4981 aagaacgcct gcctcgtgtc cgcgaagtcg ggcatcggcg ttcgcgacat gctcgaggcg
     5041 gtcgtcgagc gaattccgcc gccgaaggcc gaccgcgaag ggccgctctc cgcgctgatc
     5101 ttcgacagct ggttcgatca gttccgcggt gtcgtcgtgc tcgtccgcgt gatctccggg
     5161 acgatcaaga agggcacgaa gctcaagttc atggggaccg aggtcacgta cgagtgcacc
     5221 atgatcgcgg tgcgtcgtcc ggcgacgttc gagatcgaga cgctcgaagc agggcaggtc
     5281 ggcatcctcg cggcatcgat caaggacatc gcgcacgcac gcgtcggcga taccgtgacc
     5341 gacgccgata accccgtcga cgccccgctg cccggcttca agaccgtcaa gccgatggtg
     5401 ttcgcgggct tgttccccgt cgacgccgct gactatcaag atctcaagga cgcgctcggc
     5461 aagctcgtgc tcaacgacgc gagcgtcacg tacgagccgg agacgtcgtc ggcactcggc
     5521 tttggcttcc gcaccggctt cctcggcctt ctccacatgg agatcatcca ggagcgtctc
     5581 gagcgcgagt tcaacctgaa cctcatcacg acggcgccga gcgtgcgcta tcgcgtgaag
     5641 atgaacggcg gcgagatcgt cgagctcgac aatccggcga aggttccgga tcaaggcaag
     5701 tacgacgcga tcgaggagcc gatcatcctc gcgaccgtgc acctgacgca ggaatacgtc
     5761 gggccgatca tgcagctctg ccaggagcgc cgcggcgtgc agcagggcct tcattacgac
     5821 ccaggtggcc gcgttcgcgt tgtctacgaa atgccgctgg cagaggtcgt gttcgggttc
     5881 tacgatcgac tcaagacgat gtcgcgcggc tacgcgtcac tcgattacga accggcgggc
     5941 taccgcgaag cggatctcat tcgcctcgac gtgctcgtca acggtgagcg cgtcgacgca
     6001 ctctcgaaca tcgcgcaccg cgatagcgcg tatcaccgcg gcgtcgagct gtgcagcaag
     6061 atgaaggacg tcgtgccgcg ccagatgttc gacgtcgcga tccaagccgc catcggctct
     6121 cgcgtcatct cgcgcacgac ggtgaaagcc gcccgcaagg acgtcaccgc aaaatgctac
     6181 ggcggcgaca tcacgcgaaa gaagaagctg ctcgagaagc agaaagaggg caagaagcgc
     6241 atgaaggcgg taggctcggt cgagcttccg caggaagcgt tcttgacgat tttgaaagtc
     6301 gactgacgaa tgcgcgcctc tcgcctcgat cgccggattc gcaaagaagc ctggacgctc
     6361 gtgcgcgatg cacgcgctgc gctgtccctg aagaaggggc tgcgcggcaa aggcggagac
     6421 ctcgcgaccg tcaccggcga agtcgagaag ggcctcgtcg acaaagatat gaagcgcgtg
     6481 cgggacaact tgcccgtact cgacgcgctc gtcgacgagc tggtcaagcg cccgccgaaa
     6541 tcaacgacgc gcgactacgt cgaatcgatc ggagccgcga tcctcatcgc gctcgcgctc
     6601 cgcgcgttcg tgatcgaggc gttcaagatt ccgtcgagct cgatgtatcc gacgctcgaa
     6661 atcggcgacc acatcttcgt caacaagttc atctacggag tccggatccc gtacacgaac
     6721 atcaagttct tcgagctgcg cggcccgcat cgcggtgaag taatcgtctt ccaatatccg
     6781 tgcgatatgg accgcgacta catcaagcgc gtcatcgcgc tcgaaggcga caccgtcgag
     6841 gttcgctgca acatcgtcta cgtcaacggt aaggcgattc cgagcaagat ggtccaagac
     6901 ggggaccatt gctcgtacaa ggactacgac gagcgcgagg accgctggtt catgcgcaag
     6961 tgcagccgtt atcaagagac cgtcgacggc gagccgtacg acacgttcca cgatgacgag
     7021 cggcccaagc gcgagctagc gatcgcgcgc ggcgagcaga tcaatgacca gcgcgatttc
     7081 ccctcggcgg gctcgcgcct cattccgagc tgcgtgcagg ccgaagagcc gggccagtcc
     7141 gcggagaacg tcccgcaggt caaaggcacg ctgatcgaga tcaagcccga gtccgtcgcg
     7201 acggcgtgcg agcagcagct gcagtacaaa gtccccgcgg gtcacgtctt cgtgatgggc
     7261 gacaatcgca acaactcgaa cgactcgcgc atctggggct cggtgccgat cgagaacatc
     7321 aagggcaagg ccctcttcat ctggctctcg tacgagcact gggggccgct cgattggggc
     7381 ggcatccgct ggtaccggat cggaaacttc gttcgctgat cggcgcgcgc gaagtggtta
     7441 ctgcttcttc tgcggcagtg agagcttcgc gaagcaggtc ttgagtgcgt cgtcgatcgc
     7501 gaccttcgca gcgtcgtccc acgtcagctc gcgatccttc tcgcgcactt tcatgccgag
     7561 ctcttgcttc acggtcgctg caccgtcgcc ggtgaaaccc atcgtgcggc cgggaatcgt
     7621 ctcaccgagc acgtgaacgc cgaccgacac gacgaggcgc tgtgcgttct tcttgtcctc
     7681 gagcgggacg agctcttcac tcgccgaggt gatctcgacc gtcacgaggt acgcgccgtt
     7741 gatgcccttc ttcgtgagcc acttgcggta ggggccacca ttgtccttcg cgggatcggg
     7801 cgcgccggtg atgtcggcga cgagctgcgg atgctcggcc atcgcctttt tgaacacagc
     7861 ctcgacgcgc gactgcgccg tcttgcccgc gtcacctttg acctcggcct tgagaagcac
     7921 cttggtgagc tcgaagtggt acttcttctt cggcgcagcg tcggcggcga tcggcatcgc
     7981 caaaaccagc gacagagcga acaagaacag gctcttcatc gggcgcatca tgcctgaatg
     8041 gacgctgcgt gctaacttcg attcatggtg aggccgcgtt ggcacgacgc gttgatcgtc
     8101 tgtgcaatcg tcggtctcct cggtttaggg gcttgggcgc tatggtggga cgatgtacgg
     8161 cgtgtcctgc atctgggccc cgctcctgag ggttccgagc agggtccggc gaaggcgaat
     8221 cccgctgacc ttagttctcc ccgaacttga ccgtggttac cgaggcttag taccgtttcg
     8281 gttcgggagg ggagcccagt agggtttgcc cgtccctcag gagccaatgt tcaccatcat
     8341 cattcaggaa aaaggcggcg aacagcgccg catggtgttc aacaagcctg aggtcaccat
     8401 cggccgcgta caaggcaacg acatcgtgct cccgaaaggg aacgtgtcga agcgtcacgc
     8461 gcgcatcgtg ctcaaagacg gcaagttcat catcgtcgat ctaaagagta cgaacggcac
     8521 gtacgtcaac ggtcgcaaga tcacgagccc gctcgtcgtc aaagactcgg acaagatcta
     8581 catcggcgat ttcatcgtcg gtgtcgacga agcagcagcg gaaggtgatg gaccgtcgga
     8641 gacgacgacg tcgccgcccg gatctgatcg tccgatcgat ccgcgtccgc ctgcaccgac
     8701 cactgcatca ccacaaccaa tgggcggaat gcccggcatg ggaatgggcg gtggaatggg
     8761 aggcatgggt ggaatgggtg gtcctccgga accaccgccg cctgctgcac cgccagcgcc
     8821 gagcatgccg cctccgatgg gtgcgcgtgg tcccggtggt ggacctccgc gtccgccggg
     8881 tggaccgccg cgtgatctcg gtggaccgcc gcgcgatatg gacggtccga tgggcggagg
     8941 cccgatgggc ggaggcccga tgggcggtgg cccgatgggc ggaggcccga tgggtggccc
     9001 gatgggcggt ggccccatgg gtggcccgat gggtggtggc ccgatgggcg gtccgccgcg
     9061 cgagccgccc ggtccgcgag gtcttggtgg tccgccgcgc gagccgcctg gtccgtcgcg
     9121 cgatatgccg ccgatcgcac cgccgccagc gaacgaggcg cgcacgcgtc cgccggttcg
     9181 tggtgcaggt ggaacgatgc cgcctccgat ggcgccgatt ccgtcgccga cgtccgcgcc
     9241 gccgccaccg gatccccatt tcccgccgcc ggcagcgatg ccgccgccgg gcccgatggg
     9301 tggcgcaccg atgggcaacc cgatggcaat ggcgccgcct gtggctccgc cgactgccgc
     9361 accgatgccc gtcgctgtag cagcaccttc gatcgccgcg gctccgcaag gcacgcccgc
     9421 gcaagtcgtt ccgctgcaga aggacaagtc cgcgcggacg gtgtccgccg tcggcacgca
     9481 gccgtcgaag aagatcacgg gacgtccgct ggctcccgct gcaaagcgtg gcgttcagct
     9541 cgagccgctc gacagcaaga tcgtcaagat gctcgacctc caggcgaaca tcctcgagcg
     9601 tctgcgcgcg aagctcgatc tcgatcgcgt tccgctcgag cggttgcacg aggacgagct
     9661 ctggcagcgc gccgagaagg cgacgatcga tctcgtcgag accctggaga cgagcggtga
     9721 gctgccgaag tacatcgatc aggaagcgct gatcaaaggc acgctcaacg aagcgctcgc
     9781 gctcggacct ctcgaggatc tgttccagga cgacagcatc gacgagatcc tgatcgaccg
     9841 gcgcgatcgc gtcgtcgtcg gtaagggtgg tcagctgcgc ggctccggca aggcattctc
     9901 gtcggacgag atgctcgagc gcgtcgtgaa gcgcctcgtg ttcgaagccg gctcggtgat
     9961 cgacgaatcg catccggtcg tcgacctccg catgcgcgac ggcacgcgcg tcaccgctgc
    10021 ggtggctccg gtcgctgcgc gcggcgcatg cctcgtcttg aagaagccgc ccgcatcgac
    10081 gccgacgctc gccgacctcg tgtcgcaagg cgtcctatcg aacggcatgg ccgagttcct
    10141 gacgacgtgc atcacggcgc gccgcaacgt gctcgtgtgc ggtggccccg gctcgggcaa
    10201 gaccacgctg gtcggcgccc tcgcaggtgc ggcaccggct ggtgagcgca tcgtctccgt
    10261 cgaggatgtc gcggagctcg cgatcggccg agacgagtgg atccagctcg agacgcgtcc
    10321 aacgaacggc aaaggcaacg acgtcgacct cggcgcgttg ctcgataccg cgatgcggct
    10381 catgccggat cgtctcgtcg tcggtgacgt gcgtggccgc gaggcgatgc ctctcgtcaa
    10441 cgcgctgaac tcgtcggtcg acggcgcgat cgtcggcatg tcaggcgaag gcacgagcgc
    10501 agcgctcaat cgccttgcga cgctcgcccg cgccacgtcg gcgctcggcg ctgactggac
    10561 cgcgatccgc gagctcgtcg gcacggcgtt cgagatcgtc gtgcacgtga cgcgcggcac
    10621 cgatggcacc atcaaggtgt cgtcgatcga agagctgagc ggcgtctccg acgtcgcgtt
    10681 cgagacggag caggtcttct gccacaagga cggcggcttc gccgcgagtg gcaaggtgcc
    10741 tcgcttctac tcggagctcg gcgctgacca agccgtattc cggtagcggc ggctagcgct
    10801 tcttgcgcgt gcgcgctgcg acgctggcga gcttgccaga cgcgagcagc ttgcgtagcg
    10861 cgctctccat ttgccgcgcg aacagcacgc ggacagagtc gacgccgacc gcgcgaaacg
    10921 tctccatcag cttgcccgac tcggcgatgc tgacgtgacc ctcctttacc tcgccgacaa
    10981 atagctcgac gagctcgtcg acggcacggg ataggtgctt acgcagaatg ccggaagcct
    11041 tggccgcgac ctcgaggccg ataccgacac cctcgagctt catcgcgatc gagagcagcg
    11101 cagggctacc gacgagaaag acgtcgcctt tgcgttcgat gatcttcgcg cgtccgagat
    11161 ccgcgagcag gcccggtcgc cgattgccgg caagctcgta gagctcggcc tcggtgacgg
    11221 tgcgcgcgtg gtcgcctgcc cacggcgtct gcatctgctc ttcgacgccg agccactccg
    11281 ccaagtcgac ctcgccgcgg tcgatgcgct tcatcagatc gccgatcgcg tcgatccgaa
    11341 gcccgcgatc ctggagctgc gcgatgagct tcagccgctc tagatgttga tcgccgtagt
    11401 acgcgacgcg accgcggatc gtcggcccca ttagcgcatc gcgcgattga tagaaccgga
    11461 tcgtgcggct cggtacgcgc gacttggcag cgagctcgtc gatcgtgagc ttgtcgtcgg
    11521 cgttcgtctc gtcggtcgcc atcgtgagag tcgccatcat gctgccagca cggctctctt
    11581 cgtgggaagt gcgagccgaa agatcgttcg catcgtcgtg ccgagctgac gggcaaatga
    11641 gcccttgttg tacggcaggc cataccgctg gcagatcgcc tccacgcgcg gtgcgatctc
    11701 gggataccga ttgctcggca tgtccgggaa caggtggtgc tcgatctggt ggctgaggtt
    11761 gccgctcagc acgtgaaaga gcgggctgcc ctcgatgttg cagcttccga gcagctggcg
    11821 cagataccag cgcccgcgcg tctcgccctc gatctgctgc tcggtgaaga cctgcgcgcc
    11881 gtcgggaaag tgcccgcaaa aaatgatcgc gtacgaccac aggttgcgca tgaggttcgc
    11941 gatggcgttc gcgatcacga caggcaaaaa gaacggcccc gcaagcagcg gccacaggac
    12001 gtagtccttc aacagctgcc ggctggcctt cttgccgatg cccgcgagct tgccgcgcgc
    12061 ttccgcgagc tgcccgcgct tcaccgcgcc gaggtcgacg tcatgcatcg caacgcccca
    12121 ctgaaagagc atcgcgagta gcgcgttgta gaccggctgc gcgaggtgcg cgggcttcca
    12181 gcgctgcatc tcggtgacgc gcaaaatctc atagccgacg tcgcggtctt tgccgaggac
    12241 gttcgtccac gtgtggtgca ccacgttgtg cgagtgcttc cactgatccg cgggacagac
    12301 gttgtcccac tcccacgtcg aggaatggat ctccggatcc tgcatccagt cccactggcc
    12361 atggagcacg ttgtgcccga tctccatgtt ctccaggatc ttcgagaggc cgagcgcgat
    12421 ggtgcccgca ccgatcacgc cgatgaagag tggtggcgcg ggaatgaggg gcaggctcgc
    12481 gaagatcatc atgcgccctc caaaggcgag cccgcgctgc actttgatcg cgcgcttgat
    12541 gtagcggcgg tcgctctcgc cgaggctggc cttcacctcg cgctgaatgg cgtcgagctc
    12601 gcggccaaac gcttcgatgg cgtcatcgtc gagggcaaca ggtagtgaat gagtcatgtg
    12661 aaagtccttt cagagggcga gctcgacgtc acccgccgcc gcgcagacgc acgtctgaac
    12721 acgcgtgccg ggctcggtga tgcgctcgcc agttcgcaag tcacgtacgc agccggacac
    12781 gagcgtcgtg tcgcagctgt ggcagatgcc catgcggcat ccatgaggtg cgttgacgcc
    12841 cgcctcttcg gcgatgcgaa gtagcggcgt gcgcccatcg cttcgcgcct cgcgattcgt
    12901 cgtcgagaac cggacgcggc cgccgccgtc gccgctgctg ccgccgctgg gcacggcgac
    12961 gaacgctgcg gcgaatcgct cgacgtgcaa cgccgtcgcc atgtgatggc cggcgaaaca
    13021 cgcagtgagc gtatcgagca agctctgcgg cccacacgcc cacgtctcgc gggagcgcca
    13081 atcgggcatc agcgcatgaa gccgctcgga gcacagccgt cgcgggtccg cgacggtaag
    13141 gacagtcgtc agccgatagc gcggatgctt cagcgcaagc gcgcggagct cgtcgccgaa
    13201 gatgacgccg tgcgcggtct tcgcgacgtg cacgtgcgcg atgtcaggta gctcaccgcc
    13261 cgcgtcgagc gaccgcagca tgctcatgac gggcgtgatg ccgctgcccg cagtgacaaa
    13321 gccgatgcgg tcgggcaggg cgtccggcag aacgaactcg ccgtcgggca gcccgatgct
    13381 caggtagtcg cccacgcata cgtcgcggac aagcgcattc gagaccttgc cctg
//
LOCUS       DATLBZ010000562        14206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_1489948, whole genome shotgun sequence.
ACCESSION   DATLBZ010000562 DATLBZ010000000
VERSION     DATLBZ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14206
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489948"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..933)
                     /locus_tag="VMZ53_28105"
     CDS             complement(55..933)
                     /locus_tag="VMZ53_28105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HUS32409.1"
                     /translation="MSTDWVEIIVPVGAADVDDISALVAAEIDAASAGTEQRGDEVVF
                     WVAASDVAAALSATRDAVTRWAAGGAAVDPSRVRSAAALPEAEWRDAWKKYFKVSRLT
                     RQFVVVPSWEPYTPQEGDVAIDLDPGMAFGTGTHASTRLVLEHLQQLADEGAEPARIL
                     DVGCGSGILSIAAVKRWPGASCVALDNDPIAVQATAENAAHNKVGDRIAAAITPVGDL
                     TETFPLVLANIQAHVLRELKAALLARTGGTLILSGLLTPQAAPLADEFVAEGLRLVAI
                     RQSTDDPQWSSAVLAR"
     gene            complement(1003..1233)
                     /gene="rpsU"
                     /locus_tag="VMZ53_28110"
     CDS             complement(1003..1233)
                     /gene="rpsU"
                     /locus_tag="VMZ53_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HUS32410.1"
                     /translation="MENTFGKPVEVEVRDSLEKAMKILKQKMSKEGILQELKRRRFYE
                     KPSVKKKRKTREARKRLRREMKRRVMPAPTNR"
     gene            complement(1281..1685)
                     /locus_tag="VMZ53_28115"
     CDS             complement(1281..1685)
                     /locus_tag="VMZ53_28115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32411.1"
                     /translation="MEFQQLAFGDAATREFTNPGIISVDWNWVVPQSVLEQNHFRGVL
                     GWRCVLLRRVDVARAQQIIQDEGRSMIDVTGVKVFSATKAKERELLGELITDWIRTHP
                     EHEIVDKIVTQSSDSEFHCLTITLFYKHNAKS"
     gene            1725..3107
                     /locus_tag="VMZ53_28120"
     CDS             1725..3107
                     /locus_tag="VMZ53_28120"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS32412.1"
                     /translation="MRGILGFLLVAGLTGSAAAQPATKAAPPPAAKAAQPAPKATQLP
                     PIERFTLKNGLEVAVINVDVPVAAVQLWYHAGSKDEPRDRRGSAHMFEHMMFKGTQHV
                     RPEAHAQMLNGIGGTVNAATDEDATHYINNVPPDYVDFAIQLEAERMRNLLFRKEMID
                     TEREVVKEEVRQQENSPITKGFLRFLEIAFTKHPYGWLAGGNLKDLDATTPQDLKKFY
                     DAYYQPNNAMLVVAGKTSVEQVKASAEKWFGSIPKAADASRPSKEAQEPAQTAQRRET
                     TEVSQVGITVIGWHVPSAKDKDIYAIQVASIILGAGDSSRLKVKLKATDPKTKQALAL
                     EAGVQPLIHEDPGIVLALAAYLDKAKIEPVEKAMMAEVKKLGDAGPAADELRKAKNQV
                     QSGFVFSLESAQGLAEAIGRSWIQTGDPTSFVHDVDAIEAVTAADVKRVVKQYFTPEK
                     ATVVVIPPAR"
     gene            3113..5341
                     /locus_tag="VMZ53_28125"
     CDS             3113..5341
                     /locus_tag="VMZ53_28125"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HUS32413.1"
                     /translation="MKRLLPVLALLACGPKPSPAPIPTLPTDGSEHTAKPINDTPAKP
                     AANDAWAGRNDLIQSPAAQPPKKVELPPITSFKLANGLEVFVVKSTRLPVASMQLAIK
                     AGRMQEPRARLGVAEATADMLVKGTKKHNAVALAKAIDFVGGTISADATFEATLLSCS
                     VLKTNLKTCLELMPEMLTQSTFPDDELAKIKELMVGTVRQRLDDAGSLSAAHVQNLLW
                     GNEHVRGWINSEQSVASLRRDDLLAWQKTWYAPNNALLVVSGDVDEKKLKTDLEKAFA
                     GWKQSTVPPSPSFKEPGLSGSRIRLVDKPGQTQTHIRIAQFGIKHDDARFFDTLVWNY
                     ALGGGAFSSRLMKVVRVEGGKTYGASSSFDRNLDKGSWVAQSFTRNSEAVATTKLILG
                     EIEKMEKEGPTQAEVTAAISNITGSYGTRFQSAADIGAALVGAELHGFGLEYLSNYPL
                     AVGKVDVDSAKRAASEILDAKNYVVVMVGDAKDIEPQLQKAGWRYEKVAFNAPITPEA
                     KQPDAPVDAKSLAASRQILAEALASKGGKAKIGALKALKLTATGTTTISGQNLPVDIE
                     RVFIVPDKMRIDATLAKQFKVTVGVNGKSGWQLAPDQTGKSQLQVFAGPDIVQAQFEA
                     WREPELLLLKANDKDAKLTPLADENIDGKPHAVVKLGSPYDLDVTLYIDKKSKLLSRI
                     TFSDGGGQVEDYADYKDVGGIKVAHTRKSTAQGRVTQLKIEKVEWDPKIDNAIFEKPK
                     AP"
     gene            5387..5638
                     /locus_tag="VMZ53_28130"
     CDS             5387..5638
                     /locus_tag="VMZ53_28130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32414.1"
                     /translation="MRRRRKQIITIGGTRRELSPKDALRFEVVRDVLVERISLYEGAL
                     RLGMDIDDLAELVVGARQAVIRALGEQALENFEPVAEAV"
     gene            complement(5640..6938)
                     /locus_tag="VMZ53_28135"
     CDS             complement(5640..6938)
                     /locus_tag="VMZ53_28135"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HUS32415.1"
                     /translation="MIPKSDDPFTITSPGKADGSGLLPAADQQRLEAAFETVLQKGQQ
                     TIDKLEQEIAKLEQDIAKKKQEADNLVAQIASRESSLRDDYNRNLLICAFFPNPATCI
                     FANYLANDGTLRSYQQQLDAARAAQAQMQQQLTAYQAKRNDLRARITEIRTGKDRLIA
                     MLHDNQTPSIPPELASSPDAGRAYWRATAMGQVQRAINDEIHLLVDLRNAAVELSNTL
                     DQSLATLRSLDQATQELVEQSHNQFMKLVKALLSGDPAAAADKWLEDQLASRTRALLN
                     SLEWPLNEFARYVVESDTNPNAGDQLVQRILQKLMNGGGGGGLQPVSFEASTQVNVLD
                     NTVAKSAMSVTESRTLSELEVFVDIQHTYVGDLVVSLQHGSQTYVLQNRSGGGQDNLV
                     KTFAVPQAAGQQLKGTWTLVVEDTADQDTGKLRRWQLIAQ"
     gene            complement(7158..7697)
                     /locus_tag="VMZ53_28140"
     CDS             complement(7158..7697)
                     /locus_tag="VMZ53_28140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32416.1"
                     /translation="MPLQLPRTSDPAVIEHRLLDLAYTTDATITTPLLAYYAPCSIKD
                     AEHVLDRLVTEERLQMEVDDDGNIFYVMPGRQKGVLPPATEGHTAHATHTPYALVPQV
                     HPVLELRPPPNPAAAAVLSLIVPGAGQLYAGRPLAAVAWFTLVTIGYMLLIVPGVLLH
                     ILCVASASAAANQWRPSMA"
     gene            complement(7697..9448)
                     /gene="ligD"
                     /locus_tag="VMZ53_28145"
     CDS             complement(7697..9448)
                     /gene="ligD"
                     /locus_tag="VMZ53_28145"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HUS32417.1"
                     /translation="MARRAARKSDAGASEKLAKYRSMRDFGVTAEPSGARARRPGGNS
                     FVIQKHAARRLHYDFRLELDGVLLSWSVPKGPSLKPGDRRLAVRTEDHPLDYADFEGI
                     IPHGQYGGGAVCVWDRGTWQPEGDPREGMRKGHLAFTLDGDKLQGKWHLVRTKPQGKQ
                     EGWLLFKSRDEAANENSDIVTERPESVITGRTIDEIAVAADRVWQSNRAPELSTMEKR
                     AARIAAAGKSAKKAANDESDDRAENPRKNVFRNAALANEANKRAKARGTVQPEIRSQA
                     RPDVAALVKQLPLGFELTNLDKVLYPEQGVTKAQLIAYLAVVADWMLPQLANRPLTLV
                     RCPEGRHKPCFFQKKIFAGSPKAIKTVDIKEENGETVEYMQVADMPGLIGLAQLGTLE
                     IHTWGCHSDKVERPDFMVFDLDPDPGVEWDRVALAAFEVRKRLHAIGIESWVKTTGGK
                     GLHVCSPIKRNIDWDDFKQFTKQFAEKLADDDPKSFTANMAKAARRNRIFVDYLRNGR
                     NATFITPYSPRARPNAPVAVPITWEELAHGVDPTTFTTQTVPLRLAKLKKDPWHDIDD
                     VDQAITAAAWRAVGGKR"
     gene            9514..10437
                     /locus_tag="VMZ53_28150"
     CDS             9514..10437
                     /locus_tag="VMZ53_28150"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUS32418.1"
                     /translation="MARAYIGTSGWVYAGWREHLYADTPIKKWLQVASRAFDALEING
                     SFYTQIKPETYARWYTETPDNFRFALKGHRFVSHYKRLRDCSESITRLRDQVQPLREK
                     LAAVIWQLPSNFQCKEDGGLERLEDFMRALKEWPGMKHTLELRHKSWFTEAVADILRA
                     NNVAVCMGDAPDFPMWKAVTSDMVYVRLHGHTRKYASSYSEQSLRAWADDAKRWLAEG
                     RDVHVYFDNDAEGHAVRNALRFQELVVGTAPREIPAAAWDMPSPERKPYPMASWGPSR
                     AAKARDAAATKRRAAATAPKRGKRSSTRRAG"
     gene            complement(10478..12154)
                     /locus_tag="VMZ53_28155"
     CDS             complement(10478..12154)
                     /locus_tag="VMZ53_28155"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HUS32419.1"
                     /translation="MEPTTIAAQLREIAVYFDLDGDRHRAIAYDRAARSIEAAKGLHR
                     LLDEGRLEELPHIGPKIALVIGDLARRGSSTVLEKLRAKWPAVIVELAQLPSLGVQKA
                     RKIHQAFAPKDLDAVAAAARAGLIKELPGFGALSEQKILKSIEERRTRGTREIHIDAE
                     ERANPLAHYLRAHESATRVEVAGQVRRWCEIVDHLAFAIETSRRDEVIDRLASYGLVT
                     SVDRSAEVISARLADGMRCEFHFATRAHFGWALLCATGSPEHIDQLRARAEARGVVLS
                     ALDAPEEIDVYKALGLPFLPPEVRDGSDELSAADSGDDFTDLVTLEDVTTAFHCHTTY
                     SDGKDSIEEMARAAGELGMSAITITDHSAAASYAGGLTAEQMRAQHLEIAGLKDLPVK
                     ILRGTEADILADGTIDVPQEVLPDLDLVIASVHQRYKADEDAMTQRLVTVMRQPFFKV
                     WGHALGRLVLRRDPIPVRIDEVFDAICESRAAIEINGDPYRLDLDPINARKAAARGIP
                     FVLSSDAHSMRGIKAVRWAVGMARRARIRKRQVLNALPPDALADAIRPIA"
     gene            12185..13975
                     /locus_tag="VMZ53_28160"
     CDS             12185..13975
                     /locus_tag="VMZ53_28160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32420.1"
                     /translation="MHRTAVLLAVCAAACGRVGFDALSDGASAGLDAVDASSTPRSLV
                     LPTGGQISRITFAPNGDWYALSRTAGGFRSTDQGMTWTRCGARDGTAIAVTSDGAVWM
                     SGLDVGRSVDNCATWTDTLNPRPSEAVFAEGANIWALCDTGLRKWGGMTWVVVTTPLD
                     NNAFMRMAHGGARYFIGTLTQGVMTSPDAITWTQAPLTAFASPEIRGIAAGTNATYAI
                     TAGGNNNIACSDATGTTWTNCYGAGGIAIAVDPTNNQRAMAAIYDDLGVTTNGFGAVT
                     RGIRQPSMDSAIVEDLSYLANGDIVAATDRGVFVAPSGTTNFAPRLAGLDAWDVDDIS
                     QDGDDIWVSTRGGPLHSVGGAPFTLGTSGITGNTTTRHVRVMPDGRLVAAGRNLYISA
                     DRGDTWSMLQAMNNTDGYYVADIAFRGTRMFVATGARLLVSDPPYTTYTPVTFPGGNH
                     PANDLFVAGSNVWIGTDRGLHVSQDNGASILPIASLGTRNIRSILDLPDGRMLVGTTD
                     GVWISDVAQTSFVRAGLSAVLVDGLTLVNDAVIAATAVGVRYTRDAGTTWTPLPGAET
                     IPSSATLVDDTTGQLIVGTDTRGLIRVPLP"
BASE COUNT         2519 a         4634 c         4643 g         2410 t
ORIGIN      
        1 cgccgtcggg taatccgttc gagcgcccga tctagcggcg agcgcgccga gcggtcagcg
       61 cgcgagcacg gccgacgacc actgcggatc atcagtcgac tggcgaatcg cgacgaggcg
      121 caggccttcg gcaacgaact cgtcggcgag cggcgcggct tgcggcgtga gcaggcccga
      181 gagaatgagc gtcccgccgg tgcgcgcgag cagggcggcc ttgagctcgc ggagcacgtg
      241 ggcttggatg ttcgcgagga cgagcgggaa cgtttcggtg aggtcgccaa cgggcgtgat
      301 cgcggccgcg atgcggtcgc cgactttgtt gtgcgcggcg ttctccgcgg tggcttggac
      361 agcgatggga tcgttgtcga gcgcgacgca cgacgcgcct ggccagcgtt tgaccgcggc
      421 gatcgagagg atgccggagc cgcaaccgac gtcgaggatg cgcgcgggct ctgcaccttc
      481 gtcggcgagc tgctgcaggt gctcgaggac gaggcgcgtc gaggcgtgcg tgcccgtgcc
      541 gaacgccatg ccgggatcga gatcgatcgc gacgtcgccc tcttgcggcg tgtacggctc
      601 ccagctcggc acgacgacga actgccgcgt gagccggctg actttaaaat acttcttcca
      661 cgcgtcgcgc cactcggctt ccggtagcgc ggctgccgaa cgaacacgcg acggatcgac
      721 cgcagctccg cctgccgccc agcgcgtgac ggcatcacgc gtcgcgctca gcgcggccgc
      781 gacgtcagag gcggcgaccc agaacacgac ctcgtcgcca cgttgctcgg tgccggcgga
      841 ggccgcgtcg atctctgccg cgacgagcgc cgaaatgtcg tcgacatcag cagcgccgac
      901 cggaacgatg atctcgaccc agtccgtgct catcccagtg tccccatgat caaacaacgc
      961 gcaccacgtt caatggcgcg cgcagcgaac agcaactcca acctagcggt tggtcggcgc
     1021 cggcattacg cggcgcttca tctcacggcg aaggcgcttg cgagcctcgc gcgtcttgcg
     1081 cttcttcttg acggacggct tctcatagaa gcggcggcgc tttagctcct gcaggatgcc
     1141 ctctttggac atcttctgct tcaggatctt catcgccttc tcgaggctgt cgcgaacctc
     1201 gacctcgacg ggctttccga acgtgttctc catgggggac acagtgggta cccgatcagg
     1261 aacccaagat caagcaggtc ctagctcttg gcgttgtgct tgtagaacag cgtgatcgtg
     1321 aggcagtgga actcggagtc cgagctctgg gtcacgatct tgtcgacgat ttcatgctcc
     1381 gggtgagtgc ggatccagtc ggtgatcagc tcgcccaaca gctcgcgttc cttggctttc
     1441 gtcgcggaaa agaccttcac accggttacg tcgatcatgc tccgcccctc gtcctgaata
     1501 atttgctgtg ctcgtgccac gtctactctc ctcaacaaca cgcagcgcca cccgaggacg
     1561 ccgcggaaat ggttttgttc gagcacactc tgaggcacaa cccagttcca gtcaaccgag
     1621 ataattccag gattggtgaa ctcacgggta gcggcgtctc cgaaggctag ctgctggaat
     1681 tccaaggagg ttgcggtagt gtcccgcgcg aagagggaca cctgatgcga ggaatcctgg
     1741 gctttctact cgttgcgggt ctgaccggtt cggcagccgc acaaccggcc accaaagccg
     1801 caccgccgcc ggccgccaag gccgcacaac cggcacccaa agcgacgcag ctgccgccga
     1861 tcgagcgctt cacgctcaag aacgggctcg aggtcgcggt gatcaacgtc gacgttccgg
     1921 tcgccgcggt ccagctctgg taccacgccg gcagtaaaga cgagccccgc gatcgccgcg
     1981 gttcggctca catgttcgag cacatgatgt tcaagggcac gcagcacgtg cggcccgaag
     2041 cgcacgcgca gatgctcaac ggcatcggcg gcaccgtgaa tgcggcgacg gatgaagacg
     2101 cgacgcacta catcaacaac gtcccgcccg attacgtcga cttcgcgatc cagctcgagg
     2161 ccgagcggat gcgcaatctc ctcttccgca aggagatgat cgacaccgag cgcgaggtcg
     2221 tcaaagaaga ggtacggcag caggagaact cgccgatcac gaaaggcttc ctgcgcttcc
     2281 tcgagatcgc attcacgaag catccgtacg gctggcttgc cggcggcaac ctcaaggatc
     2341 tcgacgcgac gacgccgcaa gatctgaaga agttctacga cgcgtactac cagcccaaca
     2401 acgcgatgct cgtcgtcgct ggcaagacgt ccgtcgagca ggtgaaggcg agcgcggaga
     2461 aatggttcgg ctcgatcccg aaagccgccg atgcatcgcg tccctcgaag gaggcgcagg
     2521 agccggcgca gaccgcacag cgtcgtgaga cgaccgaggt gagccaggtc ggaatcaccg
     2581 tgatcggctg gcacgtgccg tcggcgaagg acaaggacat ctacgcgatc caggtcgcat
     2641 cgatcatcct cggcgccggc gacagctcgc ggctcaaggt gaagctgaag gcgaccgatc
     2701 cgaagacgaa gcaagcgctc gcgctcgaag cgggcgtgca gccgcttatc cacgaagatc
     2761 cgggcatcgt gctcgcgctc gccgcgtatc tcgacaaagc gaagatcgag cccgtcgaga
     2821 aagcgatgat ggccgaggtc aagaagctcg gcgacgcagg tcctgccgcc gacgagctgc
     2881 gcaaggcgaa aaaccaagtg cagtccggct tcgtgttctc gctcgagtcc gcgcaaggcc
     2941 tcgccgaagc gattggccgc agctggatcc agacgggaga tcccacgtcg ttcgtgcacg
     3001 acgtcgacgc gatcgaggcc gtcaccgctg ccgacgtcaa gcgcgtcgtg aagcagtact
     3061 tcactcctga gaaggcgacc gtcgtcgtca ttccgcccgc gaggtgatcg tcatgaaacg
     3121 cctacttccc gtcctcgctc tcctcgcgtg tggccccaag ccatcgccgg cgccgattcc
     3181 gacgctgccc accgatggct ccgagcacac cgcgaagccg atcaacgaca cgcccgcgaa
     3241 gcccgccgcg aacgacgcgt gggccggacg caatgacctg attcaatcgc ccgcggcgca
     3301 gccaccgaag aaagtcgagc tgccaccgat cacgtcgttc aagctcgcga acggcctcga
     3361 ggtgttcgtc gtcaagagca cgcgcttgcc cgtggcaagc atgcagctcg cgatcaaagc
     3421 gggtcgtatg caggagccgc gcgcgcgtct cggtgttgcc gaagcaaccg ccgacatgct
     3481 cgtgaagggg acgaagaagc acaacgccgt cgcgctcgca aaggcgatcg acttcgtcgg
     3541 cggcacgatc tccgcggacg ctacgttcga agcgacgttg ctgtcgtgca gcgtgctcaa
     3601 gacgaatctg aagacgtgcc tcgagctgat gcccgagatg ctgacgcagt cgacgttccc
     3661 cgacgacgag ctcgcgaaga tcaaagagct catggtcggc acggtgcgcc agcgcctcga
     3721 cgatgccggc tcgctgtccg ctgcccacgt gcagaacctc ttgtggggca acgagcacgt
     3781 gcgcggttgg attaacagcg agcaatcggt cgcgtcgctc cgccgcgatg atctgctcgc
     3841 gtggcagaag acctggtacg cgccgaacaa cgcgctactc gtcgtctcgg gtgacgtcga
     3901 cgagaagaag ctcaagaccg atctcgagaa agcattcgcc ggttggaagc agtcgacggt
     3961 gccgccgtct ccgtcgttca aggagccggg cctctcgggc agccgcattc ggctcgtcga
     4021 caagccgggc caaacgcaga cgcacattcg catcgcgcag ttcggcatca agcacgacga
     4081 cgcgcggttc ttcgacacgc tcgtgtggaa ctacgcgctc ggcggcggtg cgttctcgtc
     4141 gcgcctcatg aaggtcgtgc gcgtcgaggg cggcaagacg tacggcgcat cgagctcttt
     4201 cgatcgcaac ctcgacaaag gctcgtgggt cgcgcagtcg ttcacgcgca acagcgaggc
     4261 ggtcgcgacg acgaagctca tcctcggcga gatcgagaag atggagaaag aaggcccgac
     4321 gcaagcggag gtcaccgccg cgatctcgaa catcacgggc agctacggca cgcgctttca
     4381 atcggccgct gacatcggcg cagcgctcgt cggcgcggag ctgcacggct tcggactcga
     4441 gtacttgtcg aactatccgc tcgccgtcgg caaggtcgac gtcgactccg cgaagcgcgc
     4501 agcgagcgag atcctcgacg cgaagaacta cgttgtcgtc atggtcggcg atgcgaagga
     4561 catcgagccg cagctgcaga aggcaggctg gcgctacgag aaggtcgcgt tcaacgcgcc
     4621 gatcacgccc gaagcgaagc agcccgacgc gccggtcgac gcgaagtcgc tcgcagcctc
     4681 gcgccagatc ctcgccgagg cactcgccag caaaggcggc aaagcaaaga tcggcgcact
     4741 caaggcgctg aagctcaccg cgacgggcac gacgacgatc agcggccaga acctgccggt
     4801 ggatatcgag cgcgtgttca tcgtgcccga caagatgcgg atcgatgcaa cgctcgcgaa
     4861 gcagttcaag gtcacggtcg gcgtcaacgg caagagcggc tggcagctcg cgccggacca
     4921 gaccggcaag tcgcagctgc aggtgttcgc cggtcccgac atcgtgcagg cgcagttcga
     4981 agcatggcgc gagcccgagc tgttgctgct gaaggcgaac gacaaggacg ccaagctcac
     5041 gccgctcgcc gacgagaaca tcgatggcaa gcctcacgca gtggtcaagc ttggctctcc
     5101 gtacgatctc gacgtgacgc tctacatcga taagaagtcc aagctgctct cgcgcatcac
     5161 gttctccgac ggcggcggcc aggtcgagga ctacgcggac tacaaggacg ttggcggcat
     5221 caaggtcgcg cacacgcgca agagcaccgc gcagggacgc gtcacgcagc tcaaaatcga
     5281 gaaggtcgag tgggatccga agatcgacaa cgcgattttt gagaagccaa aggcgccctg
     5341 atcaaaaagt ttgcccgcgc cgatcgcctg cgtgaggctg ggtcgtatga ggcggcgacg
     5401 caaacagatc atcacgatcg gcgggacgcg acgagagctg tcgccgaagg acgcgcttcg
     5461 cttcgaagtc gtccgcgatg tcctcgtcga gcgcatctcg ctgtacgagg gcgcgctgcg
     5521 actcggtatg gacatcgacg acctcgccga gctcgtggtt ggcgcgaggc aggctgtgat
     5581 tcgcgcgctc ggcgagcagg cgctcgagaa tttcgagccc gtcgccgaag cggtctgagc
     5641 tactgcgcga tcagctgcca gcggcgcagc ttgccggtgt cctgatcggc cgtgtcctcg
     5701 acgacgagtg tccacgtgcc cttgagctgc tgacccgcgg cttgcggcac ggcgaacgtc
     5761 ttcacgagat tgtcctgccc gccgcccgag cggttctgca gcacgtacgt ctgcgagcca
     5821 tgctggagcg agacgacgag gtcaccgacg tacgtgtgct ggatgtcgac gaagacttcc
     5881 agctcgctga gcgtgcgcga ctcggtgaca gacatcgcgg acttcgcgac ggtgttgtcg
     5941 aggacgttca cctgcgtcga tgcttcgaac gagacgggct gtagaccgcc gccaccgccg
     6001 ccattcatga gcttctgcag aatgcgctgc acgagctggt cgccggcgtt cgggttcgtg
     6061 tcgctctcga cgacgtaacg cgcgaactcg ttgagcggcc actcgagcga attgagcaga
     6121 gcgcgcgtgc gcgatgcgag ctgatcttcg agccacttgt cggcggctgc cgcgggatcg
     6181 cccgagagca gcgccttcac gagcttcatg aattggttgt gcgattgctc gacgagctct
     6241 tgcgttgctt gatcgagcga gcgcagcgtg gcgagcgatt gatcgagcgt gttcgacagc
     6301 tcgacggcgg cgttgcgcag atcgacgagc aagtggatct cgtcattgat cgcgcgttgc
     6361 acctggccca tcgcggtcgc gcgccagtac gcacggcccg catccggtga gctcgcgagc
     6421 tcgggcggga tcgacggcgt ctggttgtcg tgcagcatcg cgatgaggcg atctttgccc
     6481 gtgcggatct cggtgatgcg cgcgcgcaga tcgttgcgct tcgcttggta cgccgtcagt
     6541 tgctgctgca tctgcgcttg tgcggcgcgt gcggcgtcga gctgctgctg gtagctgcgc
     6601 agcgtgccgt cgttcgcgag gtagttcgca aaaatgcacg tcgcgggatt cgggaagaac
     6661 gcgcagatga ggagattgcg gttgtagtca tcgcgcagcg agctctcgcg cgaggcgatc
     6721 tgcgcgacga ggttgtcggc ttcctgcttc ttcttcgcga tgtcttgttc gagcttggcg
     6781 atctcttgct cgagcttgtc gatcgtctgc tgcccctttt gcagcaccgt ctcgaacgcg
     6841 gcctcgaggc gctgctgatc cgccgccggc aagagtcccg atccatccgc cttgccgggc
     6901 gacgtgatcg tgaacgggtc gtcgctcttc gggatcaccg aatcatcttc ggtgcacgaa
     6961 gtgacgagtg aagcggaggc gaatagaaat aggcatgaag ctagtctacg catggtggtc
     7021 ggaccgctaa gcaggttgcg tgcccgccta acgctgcgag tcatcacacg tgaccgtgat
     7081 gacctggcgc accaacggct agtcgactgg tcagtgccga cgagatggtg gcgcgatcat
     7141 cactctccgt ggcgcattca cgccatcgac ggacgccact gattcgcggc tgcgctcgcc
     7201 gatgcgacgc agagaatgtg cagcaacacg cccgggacga tcagcagcat gtagccgatc
     7261 gtaacaagcg tgaaccacgc taccgcggcg agtggacgtc ctgcgtagag ctgacccgca
     7321 cccggcacga tgaggctgag cacggcggcg gctgcgggat tcggcggcgg acgaagctcg
     7381 aggacgggat gcacttgcgg cacgagcgcg tacggcgtgt gggtcgcgtg cgcggtgtgt
     7441 ccttcggttg ccggaggaag cacgcccttc tgccggccgg gcatcacgta gaagatgttg
     7501 ccgtcgtcgt cgacttccat ctgcaggcgc tcttcggtga cgaggcgatc gagcacgtgc
     7561 tcggcatcct tgatcgagca cggcgcgtag tacgcgagca gcggtgtcgt gatcgtcgcg
     7621 tcggtcgtgt atgcgagatc gagcaagcga tgctcgatga cggcagggtc cgacgtccgt
     7681 gggagttgca gcggcatcag cgtttgcctc cgaccgcgcg ccacgcggcg gcagtgatcg
     7741 cctgatcgac gtcgtcgatg tcgtgccacg gatctttctt cagcttcgcg agccgcagcg
     7801 gaactgtttg cgtcgtgaac gtcgtcgggt cgactccatg cgcgagctct tcccacgtaa
     7861 tcggcacggc gaccggcgca ttcggacgcg cgcgcggcga gtacggcgtg atgaacgtcg
     7921 cgttgcggcc attgcgcagg tagtcgacga agatgcggtt gcggcgcgcc gcctttgcca
     7981 tgttcgcggt gaacgacttc ggatcatcgt cggcgagctt ctcggcgaac tgcttcgtga
     8041 actgcttgaa gtcgtcccaa tcgatgttgc gcttgatcgg cgagcagacg tgcaggccct
     8101 tgccgccggt cgtcttcacc cagctctcga tgccgatcgc atgcaagcgc ttgcgaacct
     8161 cgaatgcggc gagcgccacg cgatcccact cgacgccagg atccggatcg agatcgaaga
     8221 ccatgaagtc gggcctctcg accttgtcgg agtgacagcc ccacgtgtgg atctcgagcg
     8281 tgccgagctg cgcgagaccg atgaggcccg gcatgtcggc gacctgcatg tactcgacgg
     8341 tctcgccgtt ctcttccttg atatcgacgg tcttgatcgc tttcggcgag cccgcgaaga
     8401 tcttcttctg gaagaagcac ggcttgtggc gaccctcggg gcaacgcacg agcgtcagcg
     8461 gacgattcgc gagctgcggc agcatccaat cggcgacgac cgcgagatac gcgatcagct
     8521 gcgccttcgt gacgccttgc tcgggataga ggaccttgtc gagattcgtg agctcgaagc
     8581 cgagcggcaa ttgcttcacg agcgccgcga catcgggccg cgcttgcgag cggatctccg
     8641 gctgcaccgt gccgcgagcc ttcgcgcgct tgtttgcttc gttcgcgagc gcggcgttgc
     8701 gaaagacgtt cttgcgcggg ttctccgcgc ggtcgtcgct ctcgtcgttg gccgccttct
     8761 tcgcgctctt gcctgctgcc gcgattcgcg cggcgcgctt ctccatcgtc gatagctcgg
     8821 gcgcgcgatt gctctgccac acgcgatccg ctgcaaccgc gatctcgtcg atcgtgcggc
     8881 ccgtgatcac gctctccgga cgctcggtca cgatgtcgct gttctcgttc gcagcttcgt
     8941 cgcggctctt gaagagcagc cagccttctt gcttgccctg cggcttagtc cgcacgagat
     9001 gccacttgcc ctggagcttg tcgccgtcga gcgtaaaggc gaggtggcct ttgcgcatgc
     9061 cttcgcgcgg atcgccttcg ggctgccacg tgccgcgatc ccacacgcag accgcgccgc
     9121 cgccgtactg gccgtgcggg atgatgccct cgaagtctgc gtagtcgagg ggatgatctt
     9181 cggtgcgcac cgcaaggcga cgatcgccag gcttgaggct cggacctttc ggcacggacc
     9241 aactcagaag gacaccatcg agctccaatc ggaagtcgta gtgcagcctg cgcgcagcgt
     9301 gcttctggat gacgaacgag tttccgccgg gtcgccgtgc gcgtgcgcca ctaggctcgg
     9361 ccgtgacacc gaagtcgcgc atcgatcgat acttggcgag cttctccgat gctcctgcgt
     9421 cggactttcg cgcggcgcgg cgggccatac gggatgacct tgcatcgccg gtgccacgac
     9481 gcgtcggcct ggcacgtgca atcgagagct tgcgtggcgc gcgcgtatat cggaacgagc
     9541 ggttgggtct acgccggctg gcgcgaacat ctctacgcag acacgccgat caagaaatgg
     9601 ctgcaggtgg catcgcgtgc gttcgacgcg ctcgagatca acggctcgtt ctacacgcag
     9661 atcaaaccgg agacgtacgc gcgttggtac accgagactc ccgacaactt ccgcttcgcg
     9721 ctgaaaggtc accgcttcgt ctcacactac aaacgactgc gcgactgcag cgagtcgatc
     9781 actcgcctgc gcgatcaagt gcagccccta cgggagaagc tcgccgcggt gatctggcag
     9841 ctgccgagca acttccaatg caaggaagac ggcggcctcg agcgtctcga agacttcatg
     9901 cgcgcgctga aagagtggcc gggcatgaag cacacgctcg agctgcgtca caagtcgtgg
     9961 ttcaccgagg cggtcgccga catcctgcgc gcgaacaacg tggctgtctg catgggcgac
    10021 gcgccggact ttccgatgtg gaaggcggtg acgtcggaca tggtctacgt gcggctgcac
    10081 ggccacacgc gcaaatacgc gtcgagctac agcgagcaga gcctgcgcgc gtgggccgac
    10141 gacgcgaagc gctggctcgc agaaggtcgc gacgtccacg tctacttcga caacgacgcc
    10201 gagggacacg ccgtacgcaa cgcgctgcgg tttcaggagc tcgtcgtcgg caccgcgcct
    10261 cgcgaaattc cagcagccgc gtgggacatg ccgtcgcccg agcgcaagcc gtatccgatg
    10321 gcgtcgtggg ggccgtcccg cgcggccaaa gcccgcgacg ccgcagcgac gaagcggcga
    10381 gccgcggcta ccgcgccgaa gcggggcaag cgctcgtcta cgcgacgagc tggttagcga
    10441 cgagccgata gcgacgagcc gatagcgggg gagcgggtta ggcgatcggg cggatggcgt
    10501 cggcgagtgc gtcgggcggc agcgcgttga gcacctgacg tttgcgaatg cgagcgcggc
    10561 gcgccatgcc gacggcccat ctcactgctt tgatcccgcg catcgagtgc gcgtcgctcg
    10621 acagcacgaa cgggatcccg cgcgcggcgg ccttccgcgc gttgatcgga tcgaggtcga
    10681 ggcggtatgg atcgccgttg atctcgatcg cagcgcgcga ctcgcagatc gcgtcgaaga
    10741 cttcgtcgat gcggacgggg atgggatcgc gacgcagcac gaggcggccg agcgcgtggc
    10801 cccagacttt gaagaatggc tggcgcatga cggtgacgag tcgctgcgtc atcgcgtctt
    10861 cgtcggcctt gtagcgttgg tgcacgctcg cgatcacgag gtcgagatcg ggcaagactt
    10921 cttgcgggac gtcgatcgtg ccgtcggcga ggatgtccgc ctcggtgccg cgcaggatct
    10981 tcacgggcag atccttcagg cccgcgatct cgaggtgctg cgcgcgcatt tgctcggcgg
    11041 tgaggcctcc cgcgtacgat gccgccgccg agtgatcggt gatcgtgatc gcgctcatgc
    11101 cgagctcgcc ggcggcgcgc gccatctctt cgatcgagtc cttgccgtcg ctgtacgtcg
    11161 tgtgacagtg gaaggcggtc gtcacatcct cgagtgtgac gagatcggtg aagtcatcgc
    11221 cgctgtcggc cgcagagagc tcgtcgctac cgtcgcggac ttcgggcggc aagaagggca
    11281 ggccgagcgc tttatagacg tcgatctcct cgggcgcgtc gagcgcggac agcacgacac
    11341 cgcgagcttc ggcccgcgcg cggagctggt cgatgtgctc gggcgagccg gtcgcgcaca
    11401 gcagtgccca gccgaagtgc gcgcgcgttg cgaaatgaaa ctcgcagcgc atgccgtcgg
    11461 cgagccgcgc cgagatgact tccgcggagc gatcgacgga cgtgacaagt ccgtagctcg
    11521 cgagccggtc gatcacctcg tcgcggcgcg acgtctcgat cgcgaacgct agatggtcga
    11581 cgatctcgca ccaccgacgc acctgtcccg caacctcgac gcgcgttgcc gactcgtgcg
    11641 ctcgcaagta atgcgcgagt gggttcgcgc gctcttcggc gtcgatatga atctcgcgcg
    11701 tgccgcgcgt gcgccgctct tcgatcgatt tcaggatctt ctgctcgctg agcgcgccga
    11761 agcccggcag ctctttgatc aaccccgcgc gtgcggccgc ggccaccgca tcgagatcct
    11821 tcggcgcgaa tgcctgatgg atcttgcgcg ccttctgcac gccgagcgac ggcagctgcg
    11881 cgagctcgac gatcaccgcc ggccacttcg cacgcagttt ctcgaggaca gtgctcgatc
    11941 cgcggcgcgc cagatcaccg atcacgagcg cgatcttcgg tccgatatgc ggcagctctt
    12001 cgagccggcc ctcgtccagt agccgatgca gacccttcgc cgcttcgatc gagcgcgccg
    12061 cacgatcgta agcaattgcc cgatggcgat cgccgtccaa atcgaagtag accgcgatct
    12121 cgcgtaactg cgctgcgatc gtggtcggct ccatacctgc ggtgtagcag gttacgatgg
    12181 agtggtgcat cgcacggcgg tcttgctcgc agtctgcgca gctgcctgcg gtcgcgtcgg
    12241 attcgatgcg ctctcggacg gcgcgtccgc aggcctcgac gccgtcgacg cgagctccac
    12301 accgcggtcc ctcgtgctcc cgaccggtgg ccagatctcc cgcataacct tcgcgccgaa
    12361 cggcgactgg tacgcactct cgcggaccgc gggcggattt cgttcgaccg atcaaggcat
    12421 gacgtggacg cgctgcggag ctcgcgatgg caccgcgatc gccgttacgt ctgacggcgc
    12481 cgtgtggatg tcgggcctcg acgtcggccg ctcggtcgat aactgcgcga cgtggacgga
    12541 cacgctcaac ccgcgcccct cggaggccgt gttcgcagaa ggcgcaaaca tctgggctct
    12601 ctgcgacacc ggccttcgca aatggggcgg catgacatgg gtcgtcgtga cgacgccgct
    12661 cgacaacaac gccttcatga ggatggcgca cggcggcgcg cgctatttca tcggcaccct
    12721 cacgcaaggc gtgatgacgt cgcccgacgc gatcacgtgg acgcaagcac cgctcaccgc
    12781 attcgcgtcg ccagaaatcc gcggcatcgc ggcgggcacg aacgccacgt acgcgatcac
    12841 cgctggcggc aacaacaaca tcgcgtgttc cgacgccacc ggcacgacat ggacgaactg
    12901 ctacggagcg ggcggcatcg cgattgcggt cgatccgacg aacaaccagc gcgcgatggc
    12961 cgcgatctac gacgacctcg gcgtgacgac gaacggcttc ggcgcggtca cgcgtggcat
    13021 tcgccagccg tcgatggact ccgcgatcgt cgaggacctc agctacctcg caaacggcga
    13081 catcgtcgct gcgaccgatc gcggcgtctt cgtcgcgccg tcgggcacga ccaacttcgc
    13141 accgcgcctg gccggccttg atgcatggga cgtcgacgac atctcgcaag acggtgacga
    13201 tatctgggtg tcgacgcgcg gaggcccact gcactccgtc ggcggtgcgc cgttcacgct
    13261 gggcacgtcg ggcatcacgg gcaacacgac gactcgccac gttcgcgtga tgccggacgg
    13321 ccgtctcgtc gccgcgggac gcaatctcta catcagtgcc gaccgcggtg acacctggag
    13381 catgctgcaa gcgatgaaca acacagacgg ctactacgtg gctgacatcg cgttccgcgg
    13441 cacgcgcatg ttcgtcgcaa cgggtgcgcg gctgctcgtc tcggacccgc cgtacacgac
    13501 gtacacgccg gtcacgtttc caggcggcaa ccaccccgca aacgatctat tcgtcgccgg
    13561 ctcgaatgtc tggatcggta ccgaccgggg gcttcacgtc tcgcaagaca acggcgccag
    13621 cattctcccg atcgcatctc tcggtacgcg caacattcgc agcatcctcg acctcccaga
    13681 tggccgcatg ctcgtcggta cgacggacgg tgtctggatc tcggacgtcg cgcaaacgag
    13741 cttcgtgcgc gcgggtcttt cggcggtgct cgtcgatggg ctgacgctcg tcaacgacgc
    13801 ggtcatcgcc gcaacggcgg tcggcgttcg ctacacgcgc gacgcgggca cgacgtggac
    13861 gccgctgccc ggcgccgaaa cgatcccgtc gagcgcaacg ctcgtcgacg acacgacggg
    13921 ccagctcatc gtcggcacgg acacgcgcgg cttgatccgc gtgccgctgc catagccgcc
    13981 gctggccgag ctcgcgatcg acaacgcgcg accagcggac aacggatagc gaaacgccgt
    14041 gatcagcgaa acgccgtgat cagcgaacgc cgccatcgcc gcgtcgaccg ccgccgccac
    14101 caacacccgc atcgccgcga ccaccaccgc caccaccgac gcccgcgtcg ccgcgaccgc
    14161 caccaactcc ggcatcgccg cgaccaccgc cgccaccacc gacgcc
//
LOCUS       DATLBZ010000563         6348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6332873, whole genome shotgun sequence.
ACCESSION   DATLBZ010000563 DATLBZ010000000
VERSION     DATLBZ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6332873"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VMZ53_28165"
     CDS             complement(<1..660)
                     /locus_tag="VMZ53_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HUS32421.1"
                     /translation="MQRELIQLGVAIVVILLVLLVLRRASSKSRAKADIGARGRLGLE
                     EPTGQKQRKRPIKSAKTAERVEEPEEVEADLPTDAETAAAYKAGLAKTRGGFVAKIGK
                     LFGKKQIDATTVEELEEVLFTADIGPRAADRIFQSVKTGLSKADLENPTKIWKQIRKT
                     SRDILAVDSKPFDLAAHKPFVLLILGVNGVGKTTTIGKLAKKWSLDGKKVLLAAGDTF
                     RA"
     gene            complement(679..2421)
                     /locus_tag="VMZ53_28170"
     CDS             complement(679..2421)
                     /locus_tag="VMZ53_28170"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS32422.1"
                     /translation="MRWALFICLAVAVAQAGPQVEIRAHTQLSFTGAQKRDNGEVEVS
                     GRLTDKLTGDGIASERVYVTVNGQMFPAFTSADGSFHLVVPAPSGAIDVAMTYRGGKS
                     LEGAQPLTVSTDPAKVPLHLDIVKVVDDPAGARIKVNASTIEDAALDVPVKLSVAPVG
                     EEDFKLVRTVQTGDEITFTRTEAGGAGTHRLRATFVGDDTRQAAEATVTIELTSGTTT
                     TMRLSTTKLAFEDELDVVGHVVDDDKHPVERASVTLMAGDRRLAQTATDKNGRYSFEV
                     EAQIIAAVPLPPTQKGTKVENEGGQFGIQVKADPDVPAMRASHSDPVVITIAAPQPVP
                     VSWTIVAFIATVAAAGGFFAARTKPWTKFRKRVPPAEVPSEEGDVVEQIKGGLVVAKP
                     GVVATLRRPQDDGFSGVVRDTVRGRPISDAVVTLILGARVTETGTPVPNERSTRTTLD
                     GSFAIENLGPGEWRAEVAAPGHVTERFGVTIPHRGELRGVRVDLVPVRERVFQLYRRA
                     AEPVLPESKLWGIWSPRMIVDHVRSKRPSPALADLTDFVEEVYFSPRMAAETVISEAN
                     QRVDRAIGERAARR"
     gene            2509..>6348
                     /locus_tag="VMZ53_28175"
     CDS             2509..>6348
                     /locus_tag="VMZ53_28175"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS32423.1"
                     /translation="MRIKRVEIIGFKSFCDRAVVNIGESITGVVGPNGCGKSNIVDAI
                     RWCMGEQSAKHLRGKAMEDVIFAGSESRGPAPMAEVSLTFDDVGFSHEKLELARHSEE
                     AETERALDGIAPVEAEAPVDGEAPAVDAAPADQEKPVPIVHEQAVWVDAEGKPIPTPQ
                     DEVAQLLSDAPPAFDFARYTEVTITRRLYRDGTSQYFINKTPCRLRDITDFFLGTGVG
                     TKAYAIIEQGRIGQIVSSRPQDRRAIIEEAAGITKFKVKKKAAEKKLDQTRQNLMRVS
                     DIVSELDKRMGTLRRQAQKAERYRKYKSELKDIELWKASHRWLELHAEDKRTAETLGE
                     TRTKLEDARSTWTAKDAHVIAERAEMSVEERRLVGVQEHVYELDNRIRLGESKIGFER
                     READELDARIATARQEIDEITAQRERGATELTERRTELEGLAAEVERESAEVSAREQA
                     ATDARQRMADLQRDLDGARSGLAARTTEVATSDAQIEALARRKDEAARRLERVMQETE
                     QHRDRAKELEREGRKVDEALIGLRQTRLDLGNQTEGFAARRDLLADEAARGETEVETL
                     RTELHRRKSRLQSLVEIQDRYEGFQRGTRAVMQHTVDIAPSVGGDAIRGVVADVVRAP
                     ELLEVAVEAALGDRLGGVLVSEPEVGIAAIGFLKSAGGGRSAFVPLETRSAVPQPVAP
                     TPSSPSRYESGPARFEGEGGGSSWMSPSWETFGSPTETPAPKSSAIEFEDRSALVSAS
                     IAMAGTDAVAAGEGVLGRMVDLVDFADGYEAVGKRLLGKTVVVDQLDRALRLHERGVK
                     DRLVTLDGDIVDEDGVVAGGSRDAQGAGVLAQKREIRDLEEIVGKLEHDLSESMARLV
                     TAKTELKQVGKALEGLRTQVHEGEVAIMGHEKDETRVRSELERHRDRLGQLGNEQLEL
                     EHRIRAIEIDDAAARERKQQAIDRIAELERAQLDLISEVASQREQLDALTTSLTEARI
                     RAAQLGEKRAAAESATLRLQTMDNELATRADRLAHEIEDSVRRTADLRAGCDALSAEL
                     EEVRTQRQTEVEELDRGRVAYDQRLQALTEVELQARELRTLSDRLASELTQLELRAGQ
                     IAMHKGNVLNHIDERYQIDITTVIYDFHQRPNIGEVEEARLAELKDLIDRMGSDINLT
                     AIEEFDEVSKRFEFLSAQKIDLERAVDQLQRAIDKINKTSRKLFKDTFTAVNNTFKEV
                     FPRLFRGGQASLSLSGGEGVDLLEAGVEIMAQPPGKKNSTVDQLSGGEKALTAVALVF
                     SIFLIKPSPFCILDEVDA"
BASE COUNT         1117 a         2082 c         2166 g          983 t
ORIGIN      
        1 ggcgcggaac gtatcgccgg cggcgagcag gaccttcttg ccgtcgagcg accacttctt
       61 cgcgagcttg ccgatcgtgg tcgtcttgcc aacgccgttg acgccgagga tcaagaggac
      121 gaacggcttg tgggccgcga ggtcgaacgg cttggaatcg acggcgagga tgtcgcgcga
      181 ggtcttccgg atctgcttcc agatcttggt cgggttctcg aggtcggcct tcgagaggcc
      241 ggtcttcacc gactggaaga tgcgatcggc ggcgcgcggc ccgatgtcgg ccgtgaacag
      301 gacctcctcg agctcctcga cggtggtcgc atcgatctgc ttcttgccga acagcttgcc
      361 gatcttggca acgaagccac cgcgcgtctt cgcgaggcct gccttgtagg ccgccgcggt
      421 ctccgcatcg gtagggaggt ccgcttcgac ttcttcgggt tcttcgactc tttcggcggt
      481 cttggccgac ttgatcgggc gcttgcgctg cttctgcccg gtcggttctt cgagaccgag
      541 gcgtccgcga gcaccgatgt ccgcttttgc acggctcttg gagctcgccc gtcgcagcac
      601 gagcagcacg agcaggatca cgacgatcgc aacgccgagc tggatcagct ctctctgcac
      661 ggaggcctta taccgtactc accggcgtgc ggcgcgttcc ccgatcgctc gatcgacgcg
      721 ctggttggct tcggagatga cggtttcggc cgccatacgc ggggagaaat agacttcttc
      781 gacgaagtcc gtgaggtcgg cgagagccgg gctcgggcgt tttgaacgga cgtgatcgac
      841 gatcatgcgc ggcgaccaga tgccccagag cttcgactcc ggaaggaccg gctcggcggc
      901 gcggcgatag agttggaaca cgcgctcgcg cacgggcacg aggtcgacgc ggacgccgcg
      961 cagctcgccg cgatgcggga tggtcacgcc gaagcgctcg gtgacgtggc ccggtgccgc
     1021 gacttccgcg cgccattcgc cgggaccgag gttctcgatc gcgaagctgc cgtcgagcgt
     1081 ggtgcgcgtc gagcgttcgt tgggcacggg cgtgccggtc tcggtgacgc gcgcgccgag
     1141 gatgagcgtg acgacagcgt cggagatggg gcggccgcga acggtgtcgc gcacgacgcc
     1201 cgagaagccg tcgtcctggg gcctgcgcag cgtcgcgacg acgccgggct tcgccacgac
     1261 gagaccgcct ttgatctgct cgacgacgtc gccttcttcg ctcggcacct cggcgggcgg
     1321 cacgcgcttg cgaaacttcg tccacggctt cgtgcgcgcg gcgaagaatc cgccggcggc
     1381 ggcgacggtg gcgatgaacg cgacgatcgt ccagctgacg ggaacgggct gcggcgcggc
     1441 gatcgtgatg acgacgggat ccgagtgcga cgcgcgcatg gcgggcacgt ccggatcggc
     1501 tttgacttgg atgccgaact ggccgccttc gttctcgacc ttcgtgcctt tctgcgtcgg
     1561 aggcagcgga accgccgcga taatctgcgc ctcgacctcg aagctgtagc ggccattctt
     1621 gtccgtcgcg gtctgcgcga gccggcggtc gcccgccatg agcgtgaccg atgcgcgctc
     1681 gacgggatgc ttgtcatcgt cgacgacgtg accgacgacg tcgagctcgt cctcgaatgc
     1741 gagcttcgtc gtcgagagcc gcatcgtcgt ggtcgtgccg ctcgtgagct cgatcgtgac
     1801 ggtggcttcg gcggcctgcc gggtgtcgtc gccgacgaac gtcgcgcgca agcgatgcgt
     1861 gcctgcgccg ccggcttcgg tgcgcgtgaa cgtgatctcg tcgccggtct gcacggtgcg
     1921 cacgagcttg aagtcctctt cgccgacggg cgcgacggac agcttcacgg ggacgtcgag
     1981 cgccgcgtct tcgatcgtgc tggcgttgac cttgatgcgt gcgccggccg ggtcatcgac
     2041 gactttgacg atatctagat gaagcggaac cttcgccgga tccgtgctga ccgtcagcgg
     2101 ctgcgcgcct tcgaggctct tgccaccgcg gtacgtcatc gcgacatcga tcgcgccgct
     2161 cggtgcaggc acgacgaggt ggaacgaacc gtcggccgac gtgaacgccg gaaacatctg
     2221 gccgttgacc gtgacgtaga cgcgctcgct cgcgattcca tcgccggtca gcttgtcggt
     2281 gagccggccg ctgacctcga cttcaccgtt gtcgcgcttc tgcgcgcctg tgaatgacag
     2341 ctgcgtatgc gcgcggattt ccacctgggg acccgcctgc gcgacggcca ccgcgaggca
     2401 gatgaagagc gcccagcgca cgcctgggtg atagcacgcg ccggatgtca tagggtcctg
     2461 gcaccctgcc gcggccaccg ggagaaccct cctggcgcac ggagttacat gcgcatcaag
     2521 cgagtcgaga tcatcggctt caagtcgttc tgcgaccgag ccgtcgtcaa catcggcgag
     2581 tccatcacgg gggttgtcgg gccgaacggc tgcggcaagt cgaacatcgt ggacgcgatc
     2641 cggtggtgca tgggtgagca gagcgccaag caccttcgcg gcaaggcgat ggaagacgtc
     2701 atcttcgcgg gcagcgagtc gcgtggcccc gcgccgatgg ctgaagtgtc actgacgttc
     2761 gacgacgtcg gcttctcgca cgagaagctc gagctcgcgc ggcattcgga agaggcggag
     2821 accgagcgcg cactcgatgg catcgcaccg gtggaagccg aggcgcccgt cgacggtgaa
     2881 gcaccggcag tcgatgctgc gccggccgat caggagaagc cggttccgat cgttcacgag
     2941 caggcggtgt gggtcgatgc cgagggcaag cccattccga cgccacaaga tgaagtcgcg
     3001 cagctgctct ccgatgcgcc gcccgcattt gatttcgcgc ggtacaccga agtcacgatc
     3061 acgcgtcgcc tctatcgcga cggcacgagc cagtacttca tcaacaagac gccgtgccgc
     3121 ctccgcgaca tcacggactt cttcctcggt accggcgtcg gaacgaaggc gtacgcgatc
     3181 atcgagcagg gccgcatcgg tcagatcgtc agctcgcgcc cgcaggatcg ccgcgcgatc
     3241 atcgaagagg ctgccggcat caccaagttc aaggtgaaga agaaggcggc ggagaagaag
     3301 ctcgatcaga cgcggcagaa tttgatgcgc gtctcggaca tcgtgagcga gctcgacaag
     3361 cggatgggca cgctgcgccg gcaggcgcag aaggccgagc gctatcgcaa gtacaagtcg
     3421 gagctgaagg acatcgagct gtggaaggcg tcgcatcgct ggctcgagct gcacgccgag
     3481 gacaagcgca cggcggagac gctcggcgag acgcgcacga agctcgaaga tgcgcgcagc
     3541 acgtggacgg cgaaggacgc gcacgtcatc gcggagcgcg ccgagatgtc cgtcgaggag
     3601 cggcggctgg tcggcgtgca ggagcacgtc tacgagctcg ataaccgcat tcgcctcggc
     3661 gagagcaaga tcggattcga gcggcgcgag gccgacgagc tcgacgcacg catcgcgacg
     3721 gcgcggcaag agatcgacga gatcacggcg cagcgcgagc gcggagcgac ggagctgacc
     3781 gagcgccgca cggagctcga aggcttggcc gccgaggtcg agcgcgagtc ggctgaagtc
     3841 tcggcgcgcg agcaagcggc cacggacgca cgccagcgga tggccgacct gcagcgcgac
     3901 ctcgacggtg cgcgctcggg attggctgca cggacgaccg aagtcgccac gtcggatgcg
     3961 cagatcgaag cgctcgcacg tcgcaaagac gaagcggctc gccgcctcga gcgcgtcatg
     4021 caggagaccg agcaacatcg cgatcgcgcg aaggagctcg agcgcgaagg ccgcaaggtc
     4081 gacgaagcgc tgatcggcct gcgtcagacg cgcctcgatc taggcaatca gacggagggc
     4141 ttcgcggccc gtcgcgacct gctcgccgac gaagcggcgc gtggtgagac cgaagtcgag
     4201 acgctgcgca ccgagctgca tcgccgcaag tcgcgcctgc agtcgctcgt cgagatccaa
     4261 gatcgctacg aaggcttcca gcgcggcacg cgcgcggtca tgcaacacac cgtcgacatt
     4321 gcgcccagcg tgggtggcga tgcgattcgc ggcgtcgtcg cagatgtcgt gcgtgcgccc
     4381 gagctgctcg aggtcgcggt cgaagccgcg ctcggcgatc gcctcggtgg cgtgctcgtc
     4441 agcgagccgg aagtcggcat cgcggcgatc ggcttcttga agtcggccgg cggtgggcgc
     4501 agtgcgttcg tgccgctcga gacgaggtct gcagttccgc agccggtggc gccgaccccg
     4561 tcatcgccct cgcgctacga gtcgggaccg gcgcggttcg aaggtgaagg tggcggctcg
     4621 tcgtggatgt cgccgtcgtg ggagacgttc ggctcgccga cggagacgcc tgcgccgaag
     4681 tcctccgcga tcgaattcga agatcgcagc gcgctcgtgt cggcgtcgat cgcaatggcg
     4741 ggcaccgatg cggtcgcagc cggcgagggc gtcctcggcc gcatggtaga cctcgtcgat
     4801 tttgccgatg gctacgaagc cgtgggcaag cgcctgctcg gcaagaccgt cgtcgtcgat
     4861 cagctcgatc gcgcgctgcg cctgcacgaa cgaggcgtga aagaccgcct cgtcacgctc
     4921 gatggcgaca tcgtcgacga agacggcgtc gtcgcgggtg gctcgcgcga tgcgcaaggc
     4981 gccggtgtcc tcgcccagaa gcgcgagatt cgcgacctcg aagagatcgt cggcaagctc
     5041 gagcacgacc tgtcggagtc gatggctcgc ctcgtcaccg cgaagaccga gctcaagcaa
     5101 gtcggcaagg cgctcgaagg cctgcgcacg caagtccacg agggcgaagt cgccatcatg
     5161 ggccacgaga aagacgagac gcgcgtccgc tcggagctcg agcgtcaccg cgaccggctc
     5221 ggccagctcg gcaacgagca gctcgagctc gagcaccgca tccgcgcgat cgagatcgac
     5281 gacgccgccg cgcgcgagcg caagcagcaa gcgatcgatc ggatcgcgga gctcgagcgt
     5341 gcccagctcg atctcatctc cgaagtcgcg agccagcgcg agcagctcga cgcgctcacc
     5401 acgtcgctca ccgaagcccg catccgcgcc gcccagctcg gcgagaagcg cgccgccgcc
     5461 gaatccgcga ccctccgcct ccagacgatg gacaacgagc tcgcgacgcg cgccgaccgg
     5521 ctcgcccacg agatcgaaga ctccgtccgc cggacggctg acctacgcgc cggctgtgac
     5581 gccctctccg ccgagctcga agaagtccgc acgcagcgtc aaaccgaagt cgaagagctg
     5641 gaccgcggcc gcgtcgcgta cgaccaacgc ctgcaagcgc tcaccgaagt cgagctgcaa
     5701 gcgcgcgagc tgcgcacgct ctccgaccgc ctcgcttccg agctcacgca gctcgagcta
     5761 cgcgccggcc agatcgcgat gcacaagggc aacgtgctca accacatcga cgagcgctac
     5821 cagatcgaca tcacgaccgt catctacgac ttccaccagc gcccgaacat cggcgaagtc
     5881 gaagaagcac gcctcgccga gctcaaagac ctcatcgacc ggatgggctc ggacatcaac
     5941 ctgacagcca tcgaagagtt cgacgaagtc tccaagcgct tcgagttcct ctccgcgcaa
     6001 aagatcgatc tcgagcgcgc agtcgaccag ctccaacgcg cgatcgacaa gatcaacaag
     6061 acgagtcgca agctgttcaa agacacgttc acggcggtca acaacacgtt caaggaagtg
     6121 ttcccgcggc tgttccgcgg aggccaagcg tcgctctccc tctcgggcgg agaaggcgtg
     6181 gacctactcg aagcgggcgt cgaaatcatg gcgcaaccgc cgggcaagaa gaactcgacg
     6241 gtcgatcagc tctccggcgg cgaaaaagcc ctgacggcgg tcgcactcgt cttctcgatc
     6301 ttcctgatca agccgtcgcc gttctgcatc ctcgacgagg tcgatgcc
//
LOCUS       DATLBZ010000564         3480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_5956431, whole genome shotgun sequence.
ACCESSION   DATLBZ010000564 DATLBZ010000000
VERSION     DATLBZ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5956431"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VMZ53_28180"
     CDS             complement(<1..470)
                     /locus_tag="VMZ53_28180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32424.1"
                     /translation="MRLAAHLAPTLALVACGGSGGDRWSNSLHTPRTTLVDVGAPHVP
                     VILYTRAGLSRIYVDNASHEAVAGSDKAIALDADATLDIEAGHYVVNRRGAAKLVIEG
                     VTPQSYVDVAPDRKHIALTEEHTVVIASLADGTVRRFDVPAGPARDQHLGMHWAP"
     gene            complement(467..1210)
                     /locus_tag="VMZ53_28185"
     CDS             complement(467..1210)
                     /locus_tag="VMZ53_28185"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HUS32425.1"
                     /translation="MPTAGIVIIGDEILSGKFAEENAAFLIGELRALGVDLRRITFVP
                     DDLEDIAQTVVENARRFDYVFTSGGVGPTHDDITMAAIAHGFGTTVMRHPDLEARVRG
                     YWKDKLADANLRLADVPTGAELVYGKDQIWPVIALQNVYIMPGVPTLFRRKFVDIRDR
                     FRASPVTAARLYVDVEEGELAPMLDAVVARYPAVKIGSYPRFSEKDFKVLVTLEGPST
                     DEVTGALEMLATSVGTRVVRREEPAVVGA"
     gene            1247..2542
                     /locus_tag="VMZ53_28190"
     CDS             1247..2542
                     /locus_tag="VMZ53_28190"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HUS32426.1"
                     /translation="MDTVDLVLRGGRVFDPSAARPVDQAADIRITNGRIVEIGRGLVA
                     PRIVDIKDLWVVPGLIDLHVHLREPGQEYKEDIETGTRAAAAGGFTTICCMPNTKPVN
                     DQRAITELIVRRAREVGSVRVRPIGAITHGLEGKALADIAEMKEAGIVAISDDGRPVM
                     NAGLVRRAFEYAKTFDLPLVQHAEDLDLSEGGAMNEGAVSTRIGIRGQPACAESAMVA
                     RDLEIVEWTGARYHVAHVSTARTVALVRDAKRRGLPVTCEVTPHHLALTDEACSHYDT
                     NTKCMPPLRTAADQEALLEALADGTIDAIATDHAPHSPVEKDVEFECAAPGLLGLETA
                     LPIILDFVRMGTLDDKRAIAALTGGPARAFGLPGGNLAVGAVADIAIIDPERPLTLTA
                     DAIASKSKNSPFLGQKLAGRAVMTIFEGRVAYDLDGRFR"
     gene            2539..>3480
                     /gene="carA"
                     /locus_tag="VMZ53_28195"
     CDS             2539..>3480
                     /gene="carA"
                     /locus_tag="VMZ53_28195"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HUS32427.1"
                     /translation="MTQARWPAALVLENGRVFKGEGFGGRNESIGEVVFNTSITGYQE
                     IISDPSYAGQIVVMTATQIGNVGVNKEDVEAKRVVCAGFVIRELSPIPASWRSEETLP
                     SWLERSKVPGIDGIDTRALTRVLRDEGAMRGAITPHVTEIEAVLQRVKASPKMDGLDL
                     VPGVTCRQRYEWTEPTWRATDDGRALPPPAISFHVVAYDFGMKENILRQLRDIGAKVT
                     VVPASTPARDVIALGADGYFLSNGPGDPAAVDYGIAATRELIQTGKPVFGICLGHQIL
                     GLALGARTLKLPFGHHGGNHPVLDKTTGKVEITSQNHG"
BASE COUNT          587 a         1102 c         1221 g          570 t
ORIGIN      
        1 ggggcccagt gcatgccgag gtgctgatcg cgtgcggggc cggccgggac gtcgaagcga
       61 cgcacggtac cgtcggcgag actggcgatc acgaccgtgt gctcctcggt aagcgcgatg
      121 tgtttgcggt cgggcgcgac atcgacgtag gactgcggcg tgacgccttc gatgacgagc
      181 ttcgcggcgc cgcgccggtt gacgacgtag tggccagcct cgatgtcgag cgtcgcgtcg
      241 gcgtcgagcg cgatcgcctt gtcgcttcct gcgactgcct cgtgcgaggc gttgtcgacg
      301 tagatgcggc tgaggccggc gcgcgtgtag aggatgacgg ggacgtgtgg ggcgccgaca
      361 tcgacgagcg tcgtgcgcgg ggtgtggagc gagttcgacc agcgatcacc gccgctgccg
      421 ccgcatgcga cgagggccag cgtgggagcg agatgggctg cgagtctcat gcgccgacga
      481 ccgcgggttc ttcgcggcgg acgacgcgcg tgcccacgga tgtggccagc atctcgagtg
      541 cgccggtgac ttcgtcggtg gatgggcctt cgagcgtgac gagcaccttg aagtccttct
      601 cggagaagcg cgggtagctg ccgattttga cggcggggta ccgcgcgacg accgcatcga
      661 gcatcggcgc gagctcgcct tcctcgacgt cgacgtagag gcgcgcggcg gtgacgggcg
      721 aggcgcggaa ccggtcgcgg atatcgacga acttgcggcg gaacagcgtc gggacgccgg
      781 gcatgatgta gacgttttgg agggcgatga ccggccagat ctggtctttg ccgtagacga
      841 gctcggcgcc ggtcgggaca tcggcgaggc gtaggttggc gtcggcgagc ttgtctttcc
      901 agtagccgcg gacgcgcgct tcgaggtccg gatggcgcat gacggtggtt ccgaacccgt
      961 gggcgatcgc ggccatcgtg atgtcgtcgt gggtcgggcc gacccctccg gatgtgaaca
     1021 cgtagtcgaa acgccgagcg ttctcgacga cggtttgcgc aatgtcttcg aggtcgtcgg
     1081 ggacgaacgt gatgcgacgc aggtcgacgc cgagcgcgcg cagctcgccg atcagaaatg
     1141 cggcattctc ctcggcaaat ttaccgctca ggatctcatc gccgatgatg acgatccccg
     1201 ccgtcggcat gtcgcgagca tagcagggcg tggtaagacc cgcgcgatgg acacggtcga
     1261 tctggtcctg cgtggaggcc gggttttcga tccttcggct gcacggccgg tcgatcaggc
     1321 ggccgacatt cgcatcacga atgggcgcat cgtcgagatc ggtcgcgggc ttgtcgcgcc
     1381 gcgcatcgtc gacatcaagg acctctgggt agtgccgggc cttatcgact tgcatgtgca
     1441 cctgcgcgag cctgggcagg aatacaaaga ggacatcgag acgggcacgc gcgcggcggc
     1501 cgcgggtggt ttcacgacca tctgctgcat gccgaacacg aagccggtca acgaccagcg
     1561 cgcgatcacg gagctgatcg ttcggcgcgc gcgcgaggta ggcagcgttc gcgtccggcc
     1621 gatcggcgcg atcacgcacg gcctcgaggg caaggcactc gccgatatcg ccgagatgaa
     1681 agaggcgggc atcgtcgcca tttctgatga tgggcgacct gtcatgaatg ccggcctcgt
     1741 gcgccgcgcg ttcgagtacg ccaagacatt cgacttgccg ctcgtgcaac acgccgaaga
     1801 cttggatcta tcggagggcg gcgccatgaa cgaaggcgca gtctcgacga ggattggcat
     1861 tcgcgggcag ccggcgtgcg cggagtcggc gatggtcgcg cgcgacctcg agatcgtcga
     1921 gtggaccggc gctcgctacc acgtggcgca tgtgtcgaca gcacgcacgg tcgcgctcgt
     1981 tcgcgatgcc aagcgccgcg gtctgcccgt tacgtgcgag gtcacgccgc accatctggc
     2041 gctcaccgac gaggcgtgca gccactacga tacgaacacg aagtgcatgc ctccgctgcg
     2101 caccgccgcg gatcaggaag cgttgctcga ggcactcgcc gacggcacga tcgacgcgat
     2161 cgcgacggac catgcaccgc attctcccgt cgagaaagac gtcgagttcg agtgcgcggc
     2221 gccgggattg ctcgggctcg agaccgcgct gccgatcatc ttggacttcg tccgcatggg
     2281 cacgctcgac gacaagcgcg cgatcgcggc gctgacgggc ggaccggcgc gcgcgtttgg
     2341 cttgcccggc ggcaacctcg cggtcggcgc agtcgcggac atcgcgatca tcgaccccga
     2401 gcggccgctc acgttgaccg cagatgcgat cgcgagcaag tcgaagaact cgccgttcct
     2461 cggccagaag ctcgcgggcc gcgcggtcat gacaatcttc gaaggtcgcg tcgcgtatga
     2521 cctcgacggg agattccggt gacgcaagcg aggtggcccg ccgcgctcgt gctcgagaac
     2581 ggccgcgtct tcaagggtga aggcttcggc ggtcgcaacg agagcatcgg cgaggtcgta
     2641 ttcaacacgt cgatcaccgg ctaccaggaa atcatcagcg acccttcgta cgccggtcag
     2701 atcgtcgtga tgaccgcgac gcagatcggc aacgtcggcg tcaacaaaga ggacgtcgag
     2761 gccaagcgcg tcgtgtgcgc gggcttcgtc attcgcgagc tgtcgccgat tccggcgagc
     2821 tggcgctccg aagagacgct gccgagctgg ctcgagagat cgaaagtgcc tggcatcgac
     2881 ggcatcgata cgcgtgcgct cactcgcgtg cttcgcgacg aaggtgcaat gcgcggagcg
     2941 atcacgccgc acgtcaccga gatcgaagcc gtactccagc gcgtgaaggc ctcgccgaag
     3001 atggacggcc tcgatctcgt tccaggcgtc acctgtcgcc agcgctacga gtggacggag
     3061 ccgacgtggc gagccaccga cgacggccgt gcactgccgc cgccggcgat cagcttccac
     3121 gtcgtcgcgt acgacttcgg catgaaggag aacatccttc gccagctgcg cgacatcggc
     3181 gcgaaggtga ccgtcgtccc cgcatcgacg cctgcacgcg atgtcatcgc actcggcgcc
     3241 gacggctact tcctctcgaa tggtcccggc gatccggctg ccgtcgatta cggcatcgcg
     3301 gcgacgcgcg agctgatcca gaccggcaag cccgtgttcg gcatctgtct cggtcatcag
     3361 atcctcggcc tcgcgctcgg tgcgcgcacg ctcaagctgc cgttcggtca ccacggcggc
     3421 aaccatcccg tgctcgacaa gacgaccggc aaggtcgaga tcacgtcgca gaaccacggc
//
LOCUS       DATLBZ010000565         3421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10816999, whole genome shotgun sequence.
ACCESSION   DATLBZ010000565 DATLBZ010000000
VERSION     DATLBZ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10816999"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VMZ53_28200"
     CDS             <1..375
                     /locus_tag="VMZ53_28200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32428.1"
                     /translation="LGAVAYYLLTGRRVFDGKTVLDVCLQHVTKEPTPPSQIAATHVP
                     RVLEALIMQCLAKTPAQRPASATVLAETLRDMGPFDDWDETRAIAWWKSFSSKPDYDP
                     TSTPTRTITVDIGQRVVKVGVA"
     gene            372..1943
                     /locus_tag="VMZ53_28205"
     CDS             372..1943
                     /locus_tag="VMZ53_28205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32429.1"
                     /translation="MSEGSDAETRGFLQERIALLFKTMFWSLTALVGFLAAMYWADEN
                     NAPDARFTVYGLCAVGLAAMGAIWRIVLVRRPVGMRTLHALDLVYSLGIGTAFGASAY
                     LQWDLRASGYLSAIYTTFTVFARALIVPSSGRRTLVASSLTFVPMTMAAAALALEHPQ
                     DIPGPMFFVGYLLFCVIAVALSAYGSNIIYGLRRDVSAAEQLGQYTLARKIGEGGMGA
                     VYLAHHVLLRRDTAIKRILPDKVGAENVERFEREVQHMSQLTHPNAVAVYDYGSTPDG
                     VFYYAMEYLGGGIDLENLVRKYGPQPSGRVAQILAQACGALHEAHTKKLIHRDIKPPN
                     IILCERGGMPDVAKVVDFGLVKDFAANTGASTQVVLGTPAYIAPEAVTDPSSVGPAVD
                     LYAIGCTGYFLLTGKRVFEGKTAVDVCIQHVTKAPTPPSEVTDQAIDPALEAIIMKCL
                     AKQPADRYADAGELRVALRSVPAKDWTDADARAWWKNYREIEQQVMAAPQAATMTMTI
                     DLDAHRVAPEHVKNA"
     gene            2078..>3421
                     /locus_tag="VMZ53_28210"
     CDS             2078..>3421
                     /locus_tag="VMZ53_28210"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32430.1"
                     /translation="MLYKLMFWSYVVLVGAQYVLWDVVRPPRRPEYQNEIYVVGVSGL
                     ALLAVLWRGVLLRRTLSVTWLSFMDIFVALGAGLSLAAASVLAFDYPPSHYVALIYAY
                     ITVLVRTIVVPSSGPRTFFVSCVVFVPMMIGAIILAVTADTMMPGGVFVFGAALLCGI
                     AVLLATTGSDVIYGLRRQVSQAQQLGSYKLERRIGEGGIGSVYLAHHIMLRRPTAVKL
                     LQPSRVGAETLERFEREVQHMSQLTHPNTVAVYDYGRSPDGVFYYAMEYLGGGIDLEN
                     LVRRFGPQPNGRVVEILAQVCGALGEAHESNIIHRDIKPANIMLCERGGMPDIVKVVD
                     YGLVKEITPDGNDSQVILGTPTYVAPEAVTDPSTVGPACDLYALGCVGYFLLTGRRVF
                     EGKTAVEICIQHVTAQPKRPSEVASTLIQPELETILMKCLEKKPADRYASATEMAE"
BASE COUNT          598 a         1141 c         1083 g          599 t
ORIGIN      
        1 ctcggcgccg tcgcctatta cctcctcacg ggccgccgcg tgttcgacgg caagaccgtg
       61 ctcgacgtct gtttgcagca tgtgacgaag gagccgacgc caccgtcgca gatcgcagcg
      121 acccatgtcc cgcgcgtgct cgaggcgctc atcatgcagt gcctcgcaaa gacgccggcg
      181 cagcggcccg caagcgcaac ggtgctcgcc gagacgcttc gcgacatggg cccattcgat
      241 gactgggacg agacgcgcgc tatcgcgtgg tggaagagct tctcgtcgaa gcctgactac
      301 gaccccacat cgacgcccac gcgcaccatc accgtcgaca tcggtcagcg cgtcgtgaag
      361 gtgggcgtag catgagcgag ggcagcgacg ccgagacccg cgggttcttg caagaacgca
      421 tcgcgctctt gttcaagacg atgttctgga gcttgaccgc gctcgtcggt tttctcgcgg
      481 cgatgtattg ggccgacgag aacaacgctc ccgatgcacg cttcaccgtc tacgggctat
      541 gcgccgtcgg cctcgctgcg atgggcgcga tctggcgcat cgtgctcgtg cggcgtcccg
      601 tgggcatgcg cacgctacat gcgctcgatc tcgtctactc gctcggcatc ggcacggcat
      661 tcggcgcgag cgcctatctg cagtgggacc tgcgcgcgtc cggctatctg tccgcgatct
      721 acacgacgtt cacggtcttc gctcgcgcgc tgatcgtgcc gagcagcgga cgccgcacgc
      781 tcgtcgcgag ctcgctcacg ttcgtgccga tgacgatggc ggccgccgcg ctcgcactcg
      841 agcatccgca agatattccg ggcccgatgt tcttcgtcgg atatctgctc ttctgcgtga
      901 tcgcggtggc gctctccgca tacggctcga acatcatcta cggcttgcgc cgcgacgtga
      961 gcgccgccga gcagctcggc caatacacgc tcgcgcgcaa gatcggagaa ggcggcatgg
     1021 gcgcggtcta tctcgcgcat cacgtgctgc tgcgtcgcga tactgcgatc aagcgcatcc
     1081 tccccgacaa agtcggcgcc gagaacgtcg agcgcttcga gcgcgaggtg cagcacatga
     1141 gccagctgac gcatcccaat gcggtcgcgg tctacgacta cggcagcacg cccgacggcg
     1201 tcttctacta cgcgatggag tatctcggcg gcggcatcga tctggagaac ctcgtccgca
     1261 agtacggccc gcaaccgagc ggtcgcgtcg cgcagatcct cgcgcaggcg tgcggcgctc
     1321 tgcacgaagc gcacacgaag aagctgatcc atcgcgacat caagccgccg aacatcatct
     1381 tgtgcgagcg cggcgggatg cccgacgtcg cgaaggtcgt cgacttcggc ctcgtcaaag
     1441 acttcgccgc aaacaccggc gcgtcgaccc aagtggtcct cggcacaccg gcgtacatcg
     1501 ctccggaagc agtcacggat ccgtcgagcg ttggcccggc cgtcgacctc tacgcgatcg
     1561 ggtgcacagg ctacttcctg ctgaccggca agcgcgtctt cgagggcaag accgcggtag
     1621 acgtctgcat ccaacacgtc acgaaggcgc cgacacctcc gtcggaggtc accgaccagg
     1681 cgatcgatcc ggcgctcgaa gcgatcatca tgaagtgcct cgccaagcag ccagccgacc
     1741 gctacgccga cgccggtgag ctgcgcgtag cgctccgcag cgtcccagcg aaggactgga
     1801 cagacgcgga cgctcgcgcg tggtggaaaa attaccgcga gatcgaacaa caggtgatgg
     1861 ctgccccgca ggccgcgacg atgacgatga cgatcgatct cgatgcgcat agggttgccc
     1921 cagagcacgt gaagaacgcg tgaactgggc accgactcgg caaggcgcat tacatcgtaa
     1981 tccggtggcg gatcccgggc ggtctcgacg atccattcgt gtccattggg gcgcgaacga
     2041 ggaggagtcg cgcgcgtacc tacagacccg cctgcaattg ctctacaagc tgatgttttg
     2101 gtcgtacgtc gtcctcgtcg gggcgcagta cgtgttgtgg gacgtggtgc gcccgccgcg
     2161 ccggccggag tatcaaaacg aaatctacgt cgtcggggtg agcggcctcg cgctgttggc
     2221 ggtgctgtgg cgaggcgttc tactgcgccg cacgctttcg gtcacatggc tgtcgttcat
     2281 ggacatcttc gtcgcactgg gagctgggct ctcactcgcg gctgcatcgg tgctcgcgtt
     2341 cgactacccg ccgtcgcact acgtcgcgct catctacgcg tacatcaccg tgctcgttcg
     2401 aacgatcgtc gtgccgagca gcggtcctcg gacgttcttc gtttcgtgcg tcgtcttcgt
     2461 gccgatgatg atcggagcga tcatcctcgc ggtgaccgcc gatacgatga tgcccggagg
     2521 cgtgttcgtg ttcggcgcgg cgcttctgtg cggtatcgcg gtgctcctcg ccacgaccgg
     2581 ctcggacgtc atctatggcc tgcgtcgcca ggtgagccaa gcgcagcagc tcggcagcta
     2641 caagctcgag cgccgcatcg gcgaaggcgg catcggctcc gtctacctcg cgcaccacat
     2701 catgctgcga cgcccgacgg ccgtgaagct cttgcagccg agccgcgtcg gcgccgagac
     2761 gctcgaacgc ttcgagcgcg aagtgcagca catgagccaa ctgacgcatc cgaacaccgt
     2821 cgctgtctac gactacggcc gcagtcccga tggcgtcttc tactacgcga tggagtatct
     2881 cggcggtggc atcgatctcg agaacctcgt gcgtcggttc ggcccgcagc ccaacggccg
     2941 cgtcgtcgag attctcgcgc aggtatgcgg tgcgctcggc gaagcgcacg agagcaacat
     3001 catccatcgc gacatcaagc ccgcgaacat catgctgtgc gagcgcggcg gcatgcccga
     3061 catcgtgaag gtcgtcgact acggcctcgt caaagagatc acgcccgatg gcaatgacag
     3121 ccaggtcatc ctcgggacgc cgacgtatgt cgcgccggaa gccgtcaccg atccatcgac
     3181 cgttggtccg gcgtgtgacc tctacgcgct cggctgcgtc ggctacttct tgctcaccgg
     3241 ccggcgcgtg ttcgaaggca agactgcggt ggagatctgc atccagcacg tgaccgccca
     3301 gcccaaacgg ccgtccgagg tagcgagcac gctgatccag cccgagctcg agaccatctt
     3361 gatgaagtgc ttagagaaga agccggccga ccggtacgcg agcgcgaccg agatggccga
     3421 g
//
LOCUS       DATLBZ010000566        10097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10818087, whole genome shotgun sequence.
ACCESSION   DATLBZ010000566 DATLBZ010000000
VERSION     DATLBZ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10818087"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1450)
                     /locus_tag="VMZ53_28215"
     CDS             complement(35..1450)
                     /locus_tag="VMZ53_28215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32431.1"
                     /translation="MTIYARLGLLAVALAPACDWGGQYCEGDWAAMSPEQEPATPQAR
                     VVAQILPTQTNAVDILFVIDDSKSMTDEQEQLGIWSNELFDVLGSSGELPDLHIAVTS
                     SSVAIPGLTQCDSRGGQFHVGKASLQEGDFIRDGVGPNGRVRNYTGALTDTFAKMARV
                     GDGGCGFEQPFKAARLALSTPGKFLRADALLLVVFVTDEDDCSATDGGLLFTEPYSDA
                     CSDLGTLTSYRCFEHGVRCYDGKGSRAFGDRENCHPDETSAYVESVAKFADYLKALKR
                     NPAQVVVAGIYGKPNHVSTVPDEKITSYTQPRLANVCGTGGFEGTGATPAIRMNALLA
                     KFGGRASQSSICDSELSWAMRDVGLVTRDAATRSRCLRGALLDIDAATAGVQPACRVV
                     VARDVGTRLDKQTELPPCTGSQTTRCYTIDKEAACAATDTRLAVRVDDQAVDETLTVS
                     CDVDLDAAPLHPSVGELRTDE"
     gene            complement(1530..2480)
                     /locus_tag="VMZ53_28220"
     CDS             complement(1530..2480)
                     /locus_tag="VMZ53_28220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32432.1"
                     /translation="MGRVEVVVRFAGDLLDVIQLPAGETCFVGTTAVRAVAGTELTIG
                     LVTVSVTRALADQERLRRPRIEVRPFIYGAVSLVAQVALLVVAFWTASPEPTTAPAVE
                     ADSGKKPGAVRIKRFATPSQTIERTPDPAPSETPVTADETPEQIVERDTPAPQEVQEF
                     VPSEMTGGGLATTGPSDGDGTSKFDPAANPAFDSIKVGNYSTLSSGRAAGDGYGPEAR
                     NSSLVVITCDRASCLVLGGEKAARVRKAVNERIADITDCYKRAAANGGGSVEIDFQVD
                     AAGDVGDLEIGEADPAGSCVAKILRSLQIDAADETTASST"
     gene            complement(2535..3326)
                     /locus_tag="VMZ53_28225"
     CDS             complement(2535..3326)
                     /locus_tag="VMZ53_28225"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUS32433.1"
                     /translation="MRNTIVLVLALAACGGEDMEPGVTPDAGMQISQLPDAAVPTGRC
                     PPGMVWISSTTCVDAKEATVNDYLKFINGLGAACTDPGPTCTACGGRRCFTSNSSNPW
                     HHDSTGWYIDGSVGMSLQHPARYVTYAGAKSACAAAGKRLCKAEEWTRSCGGGLKYPY
                     GNAYESGRCNSASGITGSPWAVGSHANCASASTPQLVDMSGNVWEWTNDCSGDECQVR
                     GGSFHVDGSFFPTALSCEGTQGWDQADETFGTDYDIGYRCCSAPI"
     gene            complement(3361..6936)
                     /locus_tag="VMZ53_28230"
     CDS             complement(3361..6936)
                     /locus_tag="VMZ53_28230"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32434.1"
                     /translation="MIAPGDTLANRFELRRRIGSGGMGEVYEAFDRDTAEVVALKTLA
                     RADGETVTRFKREFRALQSTSHPNLVNLRELVRDGDQWFLTMELVKGRHFLEYVKGDV
                     EKLRAALQQLVQGLRVLHEGGLIHRDIKPSNVMVNTDGRVVLLDFGLVTALDPTRQST
                     EGRATGTVEYMAPEQAVGQNVTEAADWYAVGVMLYEALTGDVPHTGHPLQIMITKQQS
                     DPRPVAELAPDAPADLRALCQGLLAIEPERRPSGAEIARRLGIDEGAGERATPISRGS
                     GRRVFIGRERELAELADSLERARERPIVHLVIGESGIGKSELVTRFTRRIHEDDQATL
                     ILNGRCYERESVPYKAFDGVADGLAQHLAHLPGAALSSLLPETPSLLVRLFPVFLRLD
                     AIASVPAPRQGAMEPQEQRRRMFESLRQLLGAISATRRVVITIDDLQWADADSFLLLR
                     ELLRGDDAPRVLVLATVRNVVDADTLSLDEMLAELADVTVRRTALGPLTEDESRALAE
                     KLAPALVDRVDVARVTREARGHPMFLHEILRHLDTLEDGTGTATLDAALTSRVAMLRD
                     DARALLELVCIAGAPISLEVASSACKLDGTTLARAIASLRVATLAREVQRSRGLALEP
                     YHDRVRESVSGRIPEAQRRELHARLAIALEASGEQGNPQLLLRHFRLAELPERAARYA
                     EEAAARSLAAHAFDQAARLWKTALNLSSGDATDRRRLLLKLGEALVSAGRGAEAAEVY
                     VEAAEGADRATRLDCHRHVAEQLLISGRIERGVASLQALLAEIGVRAPATPKAALASL
                     FQHRALLRLRGYRFKERDRSEISDAEILRLDVLQIASKGLSMVDTIRGADFQTRQLIL
                     ALRSGSRTHIAHAMCLEATFQATRGNVAQARRLFARAIELAGDNPDAYMTGLLQGGQG
                     IAAYFAGDAPRAYSLLLQAEVTMRDLSGANWEIATMKMLFMFVMRVIGDYRGMRERYR
                     QYAVEAQQRGDRYVESTMRRVCVPMWLADDNPAEGLRELARATWVPATTGYHVQHFHE
                     LIGRGEIALYTGEPADEAALQDGMKRLESSLLLRITSIRIQYEYLLGRLALAKGGAAK
                     DVERYARKLSRADNAVARVWGHVLRAGVAIRAGDTARAEKLVATAEQTAIAAGMKLTA
                     AACRLRLGQLRRDDAILTAAAMDMDALGVRVPAKMAALLIPTGKE"
     gene            complement(7018..8865)
                     /locus_tag="VMZ53_28235"
     CDS             complement(7018..8865)
                     /locus_tag="VMZ53_28235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS32435.1"
                     /translation="MGAHVLVVDDDPHILRMLSDVLGKRGFSIDTAKDGEEAYERAVA
                     RAPDLLVTDVMMPRLDGWSLVRKLRLEPQLATLPVIFLTALGGEDDRIKAFRLGADDY
                     VNKPFKLNDIVARVEKLLAAKGEAAPAAAPAAAKQTPSSGLSGDLAQVGLSTLLVLIE
                     MERKTGVMALTAADGRAGKMAVRDGKVLDAQADAAGSKRTGADAVYQMLTWSEGKFAF
                     TARDVEMEDKIRQSTTGLLMEGARLMDEAKAPEAETQLNDDAIAEWHGADERTPVVAA
                     AKLADFVRRSARASLPPYTGFSSPGIKLPPIDDPVMKADFTKPVEVAKPEPAKPEPEK
                     VEPAKAVDVAKPEPAKPEPAKVPAAARRSPVWLILALLVAGAGAATFAIKPSAKKGEA
                     VAVDVRGDAAAIGAALDRGADAAQMRATGFASMPLLRTGIETDAATLKDMAADEALFQ
                     PGAGEVIEIFQTRDGAKTSLMRTPTDAHAIALDAKTRVATANVVEVMATAPIKSQQGA
                     DAGTVAIAVAVDLKDQAAALASRVTGAKLVGLERPVELVPGAAGTSQTVPVSSKVSPN
                     LSLVVTTNAVATASPAALPGWVAPARYGAFALAGLFLLLYLVLRTRPAH"
     gene            8965..9906
                     /locus_tag="VMZ53_28240"
     CDS             8965..9906
                     /locus_tag="VMZ53_28240"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HUS32436.1"
                     /translation="MFRSTRTVLVGAMVCAIASRPAHADEVDDLVTKGEELAKRNEYS
                     QAIAAFKQADAKRPRAAHACMIGLAYMRREAWPQAELFLALCEKRAVPGDQPPDWVDA
                     AHQSLATKLREAQIPAITIEVTPRDVGARLSVSSFTADEVFEPQTIHLAPGKHVIEVT
                     APGYLRASREVTIEAGVPQTVTFALEKDPAQAPTIKVAPSPQPAVPHRHASPVPWIVM
                     GTGAVLLGTGLFYDLGVAQESRDRLASTKELDAYNVLLPEYRTQRNVTFGLLGAGVVV
                     AGVGVALKLTVFKSREAPQVSASVSGESASIVVGWQR"
     gene            9903..>10097
                     /locus_tag="VMZ53_28245"
     CDS             9903..>10097
                     /locus_tag="VMZ53_28245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32437.1"
                     /translation="MKRTALATLALTACLSVPEPPASKTHHWKELAGANQPVKLHGAR
                     MTWDDERKSIVLFGGVVDNRP"
BASE COUNT         1614 a         3491 c         3303 g         1689 t
ORIGIN      
        1 cacgccgccg gacgctggcg tcacgccgta gctactactc gtcggtgcgc agctcgccga
       61 ccgaggggtg cagcggtgcg gcatcgagat cgacatcgca gctgacggtc agcgtctcgt
      121 ccaccgcttg gtcgtcgacc cgcaccgcga gcctcgtgtc ggttgcggcg cacgcggcct
      181 ctttgtcgat cgtgtagcag cgcgtcgtct gcgagcccgt gcacgggggc agctccgtct
      241 gcttgtcgag ccgcgtcccg acatcgcgcg cgaccacgac acgacatgcc ggctgcacgc
      301 ccgccgtcgc cgcgtcgatg tcgagcagtg cgcctcgcaa gcagcggctg cgcgttgccg
      361 cgtcacgcgt cacgagcccg acgtcgcgca tcgcccatga cagctcgctg tcgcagatcg
      421 agctctggct cgcgcggccg ccgaacttcg cgagcagcgc attcatgcgg atcgcaggtg
      481 tagcgcctgt gccttcgaag ccgccggtgc cgcagacgtt cgcgaggcga ggctgcgtgt
      541 agctcgtgat tttctcgtcg gggacggtgc tcacgtgatt cggcttgccg tagataccgg
      601 cgacgacgac ctgtgccggg ttcctcttca gcgccttcaa gtagtccgca aacttcgcga
      661 ccgactcgac gtaggccgag gtctcgtcgg ggtgacagtt ctcgcgatcg ccgaacgcgc
      721 gactgccttt gccgtcgtag cagcgcacgc cgtgctcgaa gcagcgatag ctcgtcagtg
      781 ttcccaaatc ggagcacgcg tcggagtacg gttcggtgaa cagcagcccg ccatcggttg
      841 ccgagcaatc atcttcgtcc gtgacgaaca cgacgagcaa gagtgcgtcg gcgcgcagaa
      901 acttccccgg cgtcgatagt gccaaccgcg ccgctttgaa tggctgctcg aagccgcatc
      961 cgccatcacc gacgcgtgcc atcttcgcga aggtgtcggt cagcgcgccg gtgtagttgc
     1021 gcacgcgacc gttcggcccg acgccgtcgc ggatgaagtc gccttcctgg agcgacgctt
     1081 tgccgacgtg gaactggccg ccgcggctgt cgcactgagt caggcccgga atggcgacgc
     1141 tcgacgacgt caccgcgatg tggagatccg gaagctcgcc cgatgatccg agcacgtcga
     1201 acagctcgtt cgaccagatg ccgagctgct cttgctcgtc ggtcatcgat ttcgagtcgt
     1261 cgatgacgaa caggatgtcg acggcattgg tctgcgtcgg caggatttgc gcgacgactc
     1321 gcgcttgcgg cgtcgccggc tcttgctcgg gagacatcgc tgcccaatcg ccttcgcaat
     1381 attgaccgcc ccaatcgcac gcaggagcca acgcgaccgc gagcagtccg agccgggcgt
     1441 agatcgtcat ggccttatgg gactacgcgc gcgcgctttg gtggcccgct cgatcgcaac
     1501 gtgacgaacg ttgccgcttt ccgccgcggt tacgtcgacg acgcggtcgt ctcgtcggca
     1561 gcgtcgatct gtaggctacg cagaatcttc gcgacgcacg agcccgcagg atctgcctcg
     1621 ccaatctcga gatcgccgac atcgccggcc gcgtcgacct ggaaatcgat ctccacgctc
     1681 ccgccaccgt ttgccgccgc tcgcttgtag caatccgtga tgtccgcgat gcgttcgttg
     1741 acggccttgc gcacacgcgc ggccttctca ccgccgagca cgaggcagct cgcgcgatcg
     1801 cacgtgatga cgacgagcga cgagttgcgc gcttcggggc cgtagccgtc gcctgcggcg
     1861 cgcccgctcg acagggtgct gtagttgccg accttgatcg aatcgaacgc ggggttcgcg
     1921 gcagggtcga acttcgacgt gccgtcgccg tcgctcggtc cggtcgtggc gagaccgccg
     1981 cctgtcatct cgctcggcac gaactcctgc acctcttgcg gcgccggcgt atcgcgctcg
     2041 acgatctgtt cgggcgtctc gtccgccgtc acgggcgtct cgctcggcgc cggatccggc
     2101 gtgcgttcga tcgtctgcga tggcgtcgca aagcgcttga tgcgcaccgc gccgggcttc
     2161 ttgccgctgt cggcctcgac cgcgggcgct gtcgtcggct ccgggctcgc cgtccagaac
     2221 gcgacgacga ggagcgcgac ctgcgccacg agcgagaccg cgccgtagat gaacggtcgc
     2281 acctcgatgc gtggccgccg caagcgctcc tggtcggcga gcgcacgcgt cacactcacc
     2341 gtgacgagac cgatcgtcag ctcggtgccg gcgactgcac ggacggccgt cgtgccgacg
     2401 aagcaggtct caccggccgg gagctgaatg acgtcgagta gatcgcccgc aaaacggact
     2461 acgacctcga cgcgtcccat gcgcatacaa cgccgggaca gccgcaacga tctagcggtt
     2521 gctcggtttt tcgatcaaat cggcgcggag cagcagcggt agccgatgtc gtagtccgtg
     2581 ccaaacgtct cgtcggcctg gtcccagccc tgcgttccct cgcacgacag cgccgtcggg
     2641 aagaacgagc catcgacgtg gaacgagcca ccgcgcacct ggcactcgtc gccggagcag
     2701 tcgttcgtcc actcccagac gttgccggac atatcgacga gctgtggtgt gctcgcgctt
     2761 gcacaattcg cgtggctgcc gaccgcccac gggctcccgg tgatgccgct cgcgctgttg
     2821 cagcgtcccg actcgtacgc attgccatac ggatacttca gaccgccgcc gcacgagcga
     2881 gtccactcct ccgccttgca cagtcgcttg ccggcggccg cgcatgcgga cttcgcaccg
     2941 gcgtacgtca cgtagcgcgc ggggtgctgc agtgacatgc cgaccgagcc gtcgatgtac
     3001 caaccggtgc tgtcgtggtg ccacgggttg ctcgagttcg atgtgaagca ccgtcggccg
     3061 ccacatgccg tgcacgtcgg tccgggatcc gtacaagccg cgccgagacc gttgatgaac
     3121 ttcaggtagt cattgacggt cgcttctttc gcgtcgacac acgtcgtcga gctgatccac
     3181 accatgccgg gaggacagcg tccggtcggc acggccgcat ccggcagctg gctgatttgc
     3241 atccccgcgt cgggcgtgac gcccggttcc atgtcctcac cgccgcaagc cgcgagcgcc
     3301 aagacgagca cgatcgtgtt ccgcatgccc gacgaacgga cgacgcggca gaaatgggaa
     3361 ctattctttt ccggtgggga tgagcagcgc ggccatcttc gccggaacgc gaacgcccag
     3421 cgcgtccatg tccatggcag cagcggtgag aatcgcatcg tcgcggcgga gttggccgag
     3481 gcggagccgg caggctgcgg cggtcagctt catgccagcc gcgatcgccg tttgttcggc
     3541 ggtggcgacg agcttctcgg cgcgcgctgt atctcctgcg cggatcgcga caccggcccg
     3601 cagcacgtgt ccccagaccc gggccacggc gttgtcagct cgggagagct tgcgcgcgta
     3661 gcgctcgacg tccttggcag cgccgccctt cgcgagggcg agacggccga gcaggtactc
     3721 gtattggatg cggatgctcg tgatgcgaag gagcagtgag ctctcgagtc gcttcatgcc
     3781 gtcttgcagc gccgcttcat cggcgggctc accggtgtag agcgcgatct cgccgcgacc
     3841 gatcagctcg tgaaagtgct gcacgtggta gccggtggtc gcgggcaccc acgtcgcacg
     3901 cgcgagctcg cgcaggccct cggcgggatt gtcgtcggcg agccacatcg ggacgcacac
     3961 gcgtcgcatc gtcgattcga cgtagcgatc gccgcgttgc tgcgcctcga ccgcgtattg
     4021 ccggtagcgc tcgcgcatcc cgcggtagtc gccgatgacg cgcatcacga acatgaagag
     4081 catcttcatc gtcgcgattt cccagttcgc gccagagagg tcgcgcatcg tgacctcggc
     4141 ctggagcagc agcgagtacg cgcgaggggc gtcaccggcg aagtacgcgg cgatgccttg
     4201 cccgccctgc agaaggcccg tcatgtacgc gtcgggattg tcgccagcga gctcgatcgc
     4261 gcgcgcgaac aggcgccggg cctgcgcgac atttccgcgc gtcgcttgaa acgtcgcctc
     4321 gaggcacatc gcgtgggcaa tgtgagtgcg cgagcccgag cgcagcgcga ggatcagctg
     4381 gcgggtttga aaatcggcgc cgcggatcgt gtcgaccatc gacagcccct tcgacgcgat
     4441 ctgcagcacg tcgaggcgca ggatctcggc gtcgctgatc tcactgcgat cgcgctcctt
     4501 gaagcgatat ccacgcagcc gaagtagcgc acggtgttga aacagcgacg cgagcgcagc
     4561 cttcggagtc gcaggcgcgc gaacgccgat ctctgcgagc agtgcctgca acgaggcgac
     4621 gccgcgctcg atccggccac tgatcaagag ctgctcggca acgtgacgat ggcaatcgag
     4681 gcgcgtcgcg cgatcggcgc cctcggccgc ttcgacgtag acctcggccg cttctgcacc
     4741 gcgacccgcg gacacgagcg cttcgccgag cttcagcagc agccggcgcc gatcggttgc
     4801 gtcccccgac gacaaattga gcgcggtctt ccacaggcga gccgcttgat cgaacgcgtg
     4861 tgccgcgaga ctgcgtgcag ccgcttcctc cgcgtagcgg gcggcgcgct cgggaagctc
     4921 ggcgaggcgg aagtggcgca gcagcagctg agggttcccc tgctcgccgc tcgcctcgag
     4981 tgcgatcgcc aggcgcgcgt gaagctctcg acgctgcgct tccggaatgc ggcccgacac
     5041 ggattcgcgc acccggtcgt gatacggctc gagcgcgaga ccgcgactgc gctgcacttc
     5101 tcgcgcgagg gtggctacgc gcagcgatgc gatcgcgcga gcgagcgtgg tcccgtcgag
     5161 cttgcacgcg ctgctcgcga cctcgagcga aatcggcgca ccggcgatgc acacgagctc
     5221 gagcaacgcg cgcgcgtcgt cgcgcaacat cgcaacgcgc gaggtcaacg cagcgtcgag
     5281 cgtggcggtc cccgtgccgt cctcgagcgt atcgaggtgg cgcaggatct cgtgcaagaa
     5341 catgggatgg ccgcgcgctt cgcgggtcac gcgtgcgacg tcgactctat cgacgagcgc
     5401 aggcgccagc ttctccgcga gcgcccggct ctcgtcttcg gtgagcggcc cgagcgccgt
     5461 ccgccgcacg gtgacgtctg caagctcggc aagcatttcg tcgagcgata acgtgtcggc
     5521 gtcgacgacg ttgcgaacgg tcgcgagcac gagcacgcgc ggcgcgtcgt ctccacgcag
     5581 cagctctcgc aacaggagga agctgtcggc gtcggcccac tgcagatcat cgatcgtgat
     5641 cacgacgcgt cgagtggccg agatcgcgcc gagcagctgg cgaagcgatt cgaacatgcg
     5701 gcggcgctgc tcttgcggct ccatcgcacc ttgccgcggc gccggcaccg acgcgatcgc
     5761 atcgagacga agaaacactg gaaacagccg cacgagcagt gacggtgtct cgggcaacag
     5821 cgacgagagc gcagctccag gcagatgcgc gaggtgctgg gcgagcccgt cggcaacgcc
     5881 gtcgaacgct ttgtacggca ccgactcgcg ctcgtagcag cgaccattca agatcaacgt
     5941 cgcttggtcg tcctcgtgga tccggcgcgt gaaccgcgtg acgagctccg acttaccaat
     6001 ccccgactcg ccgatcacga ggtgcacgat cggccgttcg cgcgcgcgct cgagtgaatc
     6061 cgccagctcc gccagctctc gctcgcgacc gatgaacacg cgccggccac tgccgcgcga
     6121 aatcggagtc gctcgctcgc ctgctccctc gtcgatgccg agccgccgcg cgatctccgc
     6181 acccgacggg cgccgttccg gctcgatcgc caagagtccc tgacatagcg cgcgcaaatc
     6241 cgccggcgca tccggcgcga gctccgctac cggccgcgga tcgctctgct gcttcgtgat
     6301 catgatctgc agtgggtggc cggtatgcgg cacgtcaccg gtcaacgcct cgtagagcat
     6361 cacgccaacc gcgtaccaat cggcggcctc ggtgacgttc tgccccaccg cctgctccgg
     6421 tgccatgtac tcgacggtcc ccgtcgcacg gccttcggtc gattgccgcg tcggatcgag
     6481 cgcggtcacg aggccaaagt cgaggagcac cacgcgaccg tcggtgttca ccatcacgtt
     6541 cgacggcttg atgtcgcgat ggatcaggcc gccctcgtgg agcacgcgca gcccctgcac
     6601 gagctgctgc aacgccgcgc ggagcttctc gacatcgccc ttcacgtact cgagaaagtg
     6661 ccgccccttg acgagctcca tcgtcagaaa ccactggtcg ccgtcgcgca cgagctcgcg
     6721 caggttcacg agattcggat gcgacgtcga ctgcagcgcg cgaaactcgc gcttgaaccg
     6781 cgtcacggtc tcaccatccg cgcgcgccaa cgtcttgagc gcgactactt ccgcggtatc
     6841 gcggtcgaac gcctcgtaaa cctcgcccat cccgcccgac ccgatccgtc ggcgaagctc
     6901 gaaccggttc gctagtgtgt cacccggcgc gatcatcgag gcacgcgaga gtagcgcacc
     6961 gtggcggcga tcggtttgtg tgcaaattga tctacgcctg cgtggaactc tgacgggcta
     7021 gtgcgccggc cgggttcgca gcacgaggta gaggagcagg aagagtccag ccaacgcgaa
     7081 cgcgccgtag cgcgccggtg cgacccaccc cggcagcgca gccggcgacg ccgtcgcgac
     7141 ggcattcgtg gtcacgacca gcgacaggtt gggcgagacc ttcgaggaga caggcacggt
     7201 ttgcgacgtg cccgcggcgc ccggaacgag ctcgacgggt cgctcgaggc caacgagctt
     7261 cgcaccggtg actcgcgacg cgagcgccgc cgcctgatcc ttcaggtcga ctgcgaccgc
     7321 gatagccacg gttcccgcat cggcgccctg ctggctcttg atcggtgcgg tcgccatcac
     7381 ctcgacgacg ttggcggtcg ccacgcgcgt ctttgcatcg agcgcgatcg catgcgcgtc
     7441 ggtgggcgtg cgcatcagcg acgtcttcgc gccatcgcgc gtctgaaaaa tctcgatgac
     7501 ctcgccagcg ccgggctgga acagcgcttc gtcggcggcc atgtccttga gcgtcgccgc
     7561 atcggtctcg atgcccgtgc gcaacagcgg catcgatgca aatccagtcg cgcgcatctg
     7621 cgcagcatct gctccacgat cgagtgccgc tccgattgcc gctgcatcgc cgcgcacgtc
     7681 gaccgcgacc gcctcgccct tcttcgccga cggcttgatc gcgaacgtcg ccgcaccggc
     7741 gcccgcgacg agcaacgcga ggatgagcca gacgggcgac ctccgcgccg cagctggcac
     7801 cttcgccggc tccggcttcg cgggctccgg cttggccacg tcgacggcct ttgcgggctc
     7861 cactttctcg ggctcgggtt tcgctggctc cggtttggcg acctcgacgg gcttcgtgaa
     7921 gtccgccttc atcaccggat cgtcgatcgg cggcagcttg attccgggcg acgaaaatcc
     7981 ggtgtacggc ggcagcgacg cgcgcgcgct tcggcgcacg aaatcggcga gcttggcggc
     8041 cgcgacgacg ggcgtccgct cgtctgcgcc atgccactcg gcgatcgcgt catcattgag
     8101 ctgcgtctcg gcctcgggag ccttcgcctc gtccatgagg cgcgcgcctt ccatcaatag
     8161 gcccgtcgtc gattggcgga tcttgtcttc catctcgacg tcgcgagcgg tgaacgcgaa
     8221 cttgccctcc gaccacgtca gcatctggta cacggcgtcc gcgcctgtcc gcttcgatcc
     8281 ggcggcatcg gcctgcgcgt cgaggacctt gccgtcgcgg accgccatct ttcccgcgcg
     8341 cccatcggct gcggtcaacg ccatcacgcc ggtcttgcgc tccatctcga tcaacacgag
     8401 caacgtgctg agcccgacct gcgccaggtc accgctcaat cccgagctcg gcgtttgctt
     8461 cgcagcggct ggcgccgcgg cgggcgccgc ctcccccttc gcagcgagca gcttctcgac
     8521 gcgcgcaacg atgtcgttca gcttgaatgg cttgttgacg taatcgtcgg cgccgaggcg
     8581 gaacgctttg atgcgatcgt cttcgccgcc gagtgcggtc aagaagatca cgggcagcgt
     8641 cgcgagctga ggctcgagtc ggagcttacg cacgagcgac cagccgtcga gcctcggcat
     8701 catcacgtcg gtcacgagca ggtctggtgc gcgagcaacc gcgcgttcgt acgcctcttc
     8761 gccatccttc gcggtgtcga tgctgaagcc gcgcttaccg agcacgtcgc tcagcatccg
     8821 caagatatgc ggatcgtcgt ccacgacgag cacgtgcgcc cccactgcgt tcacggtatc
     8881 gcggcccgag gaaagccggc aactgttcgc cgaaaaacga ttcgtgaagc tctaggaagc
     8941 gctctgtccg ctaagatccg gtaagtgttc cgcagtactc gcaccgtcct cgtcggcgcg
     9001 atggtttgcg ccattgcgtc gaggcctgcg cacgcagacg aagtcgacga cctcgtcacg
     9061 aagggcgagg agctcgcgaa gcgcaacgag tactcgcagg cgatcgcggc gttcaagcaa
     9121 gcagacgcga aacgacctcg cgccgcgcac gcctgcatga tcggcctcgc ctatatgcgc
     9181 cgcgaggcgt ggccgcaggc cgagctgttc ctcgcgttgt gtgaaaagcg cgcggtgccc
     9241 ggcgatcagc cgcccgactg ggtcgacgcc gcgcaccaat cgctcgcgac caagctgcgc
     9301 gaggcgcaga ttcccgcgat cacgatcgag gtgactccgc gtgacgtcgg tgcgcgactc
     9361 tccgtatcga gcttcaccgc ggacgaagtg ttcgagccac agacgatcca cctcgcaccc
     9421 ggcaagcacg tcatcgaggt gactgcaccc ggttacctgc gcgcgtcgcg cgaagtgacg
     9481 atcgaggcag gcgtgccgca aacggtgacg ttcgcgctcg agaaagatcc agcgcaggcg
     9541 ccgaccatca aggtcgcacc gtctccgcag ccagccgtgc cgcatcgtca cgcgagtccc
     9601 gtgccgtgga tcgtcatggg caccggcgcg gtcctgctcg gcacgggcct cttctacgat
     9661 ctaggcgtcg cgcaagagtc tcgcgatcgg ctcgcgagca cgaaggagct cgacgcgtac
     9721 aacgtgctcc tgccggagta tcggacgcag cgcaacgtga cgtttggctt gctgggagcc
     9781 ggcgtcgtcg tcgccggcgt cggcgtcgcg ctgaagctga cggtgttcaa atcgcgcgag
     9841 gcgccgcagg tcagcgcgag cgtgagtggc gagagtgcgt cgatcgtcgt cgggtggcag
     9901 cgatgaagcg caccgcactc gcgacgctcg cgctgaccgc ctgtctctct gtgcccgagc
     9961 caccggcgag caagacgcat cactggaaag agctcgctgg cgcaaaccag ccggtcaagc
    10021 tgcacggcgc gcgcatgacg tgggacgacg agcgcaagtc gatcgtgctg ttcggcggcg
    10081 tcgtcgataa cagaccc
//
LOCUS       DATLBZ010000567         3584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10819276, whole genome shotgun sequence.
ACCESSION   DATLBZ010000567 DATLBZ010000000
VERSION     DATLBZ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10819276"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VMZ53_28250"
     CDS             <1..581
                     /locus_tag="VMZ53_28250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32438.1"
                     /translation="VTTGLFLFASFALTRASDRAVTRLGRRLEVRRAGGDTTITLDDR
                     VVRNDDVTCVRVLTSTERMPKGYGSATTFHLLLVLRDEAVLLATSGELEPVDAGARAL
                     ADVGFPRRENLARPSSLTPPGALLWAMLFSCVAAVAGVLGAMVPIIWPSGTAVVAALA
                     IAGLHVLLPWPLIALARSSYRQMFERGLGASA"
     gene            578..1255
                     /locus_tag="VMZ53_28255"
     CDS             578..1255
                     /locus_tag="VMZ53_28255"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HUS32439.1"
                     /translation="MTRRDSLDRCTECRMHTTLCICSLVPALAPRTRLALLVHHREVN
                     KPTNTGQLAARCLARSSVTVIGARTQVAVRPVVEPHEQALLLYPADDAVPIADYAASE
                     RPVVLFVPDGSWRQAHKMRRRQPGLAQVPCVILPEAAPTEYRLRGEHHDGGLATLEAI
                     ARALRILEGDAGPAIETALLDLLKIMVDRTLWLRGQLGDAQVTGGLPEAAREANPRST
                     PARRRMP"
     gene            complement(1252..2535)
                     /locus_tag="VMZ53_28260"
     CDS             complement(1252..2535)
                     /locus_tag="VMZ53_28260"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS32440.1"
                     /translation="MQTCAVCGVAVSGTEVMYTPDARVVCPRCFAASDAVGVPSGGPW
                     KGFAIAGAVMGGVPFGLSMATVSSSTVNGEVTSFVYRDWIAVICGVVALVLGLVTLWM
                     SRGETLRRGLAIGAGIGVLALGGLQIARGFGVFATPSSNSSFSTTSSITIEPPPKRVV
                     DPKRPETCFDQDSCFDLGRTLETAKDFEKAAIAYTRACEEKAVGGCFNAGFLLRKRDQ
                     AEKAHAQFQRGCDLGYVAACNEAALAYKQGKGAPKDLERARNMFEQGCSKDFALACTN
                     LADFYENALGVEADPKRAFELVAKGCDLKDEDAALACDHAGMALYEGKGVKVDKKRAT
                     SYFGKGCDLSPKYCYNLGVLAEQGDGIAKDLPKARALYEKSCKAGKPEGCNNLGHFLD
                     DGIGGSKDKAAAKELYKQACDAGVELGCKNLKATN"
     gene            complement(2595..3452)
                     /locus_tag="VMZ53_28265"
     CDS             complement(2595..3452)
                     /locus_tag="VMZ53_28265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32441.1"
                     /translation="MMRSLCLLAFVACVDDELIDEDLIDEVDASAAMAATFPGVNGEW
                     CVASPFNCRFRAPGVSQRVVTASGLDRWGIEPGASLRDGHGNVLATITNTRLTFNYGQ
                     TRELAGKAHALAMASSNGSAGWYPIERILGEQSFRARMGEVNARDPQDGRLGCYTIRD
                     GHDPEIELRKVVYDSHAEHERAGDYLSLERANGRRSANLLFMVPGFGLGGASTDHFPA
                     GTKFHRVNVPTSTGRPSITIPLWVKDGAGRYRKRHGSMTFLYGFVVGDGVKRFGWMSR
                     GALVALPEC"
BASE COUNT          574 a         1276 c         1134 g          600 t
ORIGIN      
        1 cggtgacgac cggcctgttc ctcttcgcct cgttcgcgct gacccgggcc agcgatcgcg
       61 ccgtgacccg actcggtcgc cgcctcgagg tccgccgcgc aggtggtgat acgaccatca
      121 ccctcgatga ccgagtcgta cggaacgacg acgtcacctg cgtgcgggtg ctcacctcga
      181 cggagcgcat gccgaagggg tacgggtcgg caaccacctt tcacctgctc ctcgtgctgc
      241 gcgacgaggc ggtgctgctc gccacgtcgg gcgaactcga gccggtcgac gccggagcgc
      301 gcgcgctcgc ggacgtgggc ttcccgcgtc gagagaacct tgccaggccg agctcgctca
      361 ccccgccggg tgcactgctg tgggcgatgc tgttcagctg cgtggcagcc gtggccggcg
      421 tactcggggc gatggtcccg atcatctggc cgtcaggcac tgccgtggtg gccgcgctgg
      481 cgattgccgg cctccacgtc ctgctgccgt ggccgctgat cgcgctcgca cggagctcgt
      541 accgccagat gttcgaacgc gggctcggtg cgtccgcatg actcgacggg acagcctcga
      601 ccgttgcacg gagtgccgta tgcacacgac gttgtgcatc tgctcgctcg tgccggcgct
      661 cgcaccgcgc acgcgtctcg cgttgctcgt gcatcatcgc gaggtcaaca agccgacgaa
      721 cacgggacag ctcgcggcac ggtgcctggc gcgcagcagc gtgacggtga tcggcgcgcg
      781 tacacaggtt gccgtgcgcc cggtcgtgga gccacacgag caagcgctgc tgctgtatcc
      841 ggccgacgac gcggtcccga tcgcagacta cgcggccagc gagaggccgg tcgtgctgtt
      901 cgtgccagat ggctcgtggc gtcaggcgca caagatgcga cgtcgccaac ccggccttgc
      961 acaggtgccg tgcgtgatcc tgccggaggc ggcgccgacg gagtaccgcc tccgcggcga
     1021 gcaccacgac ggcggcctcg cgaccctcga agcgatcgcg cgcgcgctgc gcatcctcga
     1081 gggagatgcc ggcccagcca tcgagacggc gctgctcgac ctcttgaaga tcatggtcga
     1141 tcgcacgctg tggctacgcg gacagctcgg cgatgcacag gtcaccggcg gcctgcccga
     1201 ggcggcgcgc gaggcgaatc cccgcagcac gcccgcccgc cgccgcatgc cctagttcgt
     1261 cgccttcaga ttcttgcagc cgagctccac gcccgcatcg cacgcctgct tgtagagctc
     1321 cttcgccgcg gccttgtcct tggacccgcc gatgccgtcg tcgaggaagt gaccgaggtt
     1381 gttgcaaccc tcgggcttac ccgccttgca agacttctcg tagagcgcgc gtgccttcgg
     1441 caggtccttc gcgatgccat cgccctgctc ggccaggacg ccgaggttgt agcaatactt
     1501 cggcgagaga tcgcagccct tcccgaagta cgaggtcgcg cgcttcttgt cgaccttcac
     1561 accctttcct tcgtagagcg ccatgccggc gtggtcgcat gcgagcgcgg cgtcctcgtc
     1621 cttcagatca catcccttgg cgaccagctc gaacgcgcgc ttcggatcgg cctcgacacc
     1681 gagcgcgttc tcgtagaagt ctgcgaggtt cgtacacgcg agggcgaagt ctttcgagca
     1741 tccctgttcg aacatgtttc gcgcgcgctc gagatccttg ggcgcgccct taccctgctt
     1801 gtaggcgagc gcggcttcgt tgcacgctgc gacgtacccg agatcgcagc cacgctggaa
     1861 ctgcgcgtgc gccttctcgg cctgatcgcg cttccggagc aggaagccgg cgttgaagca
     1921 gccgccgacg gccttctcct cgcaagcgcg ggtgtatgcg atggccgcct tctcgaaatc
     1981 cttcgccgtc tcgagcgtgc gtccaaggtc gaagcacgaa tcctgatcga agcaggtctc
     2041 gggtcgtttc ggatcgacga cccgcttcgg cggtggctcg atggtgatcg aggaggtcgt
     2101 cgagaacgag ctattcgagg acggcgtcgc gaacacgccg aacccgcgcg cgatctgcaa
     2161 gccaccgagc gcgagcacac ctatgccggc accgatcgcc agtcctcgac gcagcgtctc
     2221 gccgcgtgac atccagagcg tgacgagccc gagcaccagc gcgaccacac cgcagatcac
     2281 cgcgatccag tcgcgataca cgaagctggt gacctcgccg ttcaccgtgc tcgagctgac
     2341 ggtggccatg ctcaatccga acggcacgcc acccatgacc gcgcccgcga tcgcgaagcc
     2401 cttccacggc ccacccgatg gcacgcccac cgcgtcgctc gcggcgaagc acctcggaca
     2461 gacgacccga gcgtcgggcg tgtacatcac ctcggtcccg ctgactgcga cgccacacac
     2521 cgcgcaggtt tgcatcgcgc gatcgtcgca cgtctcgtga tcggcgaggg tgagcgagcc
     2581 gatttagtct tcgcctagca ctcgggcaag gcgacgagcg caccccgaga catccatccg
     2641 aagcgcttca cgccgtcgcc cacgacgaag ccgtagagaa aggtcatcga gccgtgccgc
     2701 ttgcggtagc gacctgcgcc atccttgacc catagcggga tcgtgatcga cgggcggccc
     2761 gtgctcgtcg gaacattcac acgatgaaac ttcgtaccgg ccggaaagtg atccgtcgac
     2821 gcgccgccga gcccgaagcc cggcaccatg aagagcagat tggccgagcg cctgccgttc
     2881 gcacgctcca gcgacaggta gtcacccgcg cgttcgtgct cggcgtggct gtcgtagacc
     2941 accttgcgaa gctcgatctc gggatcgtgc ccgtcgcgga tcgtgtaaca gccgagcctg
     3001 ccgtcctgcg gatcgcgtgc gttgacctcg cccatccgcg cgcggaacga ctgttcgccg
     3061 aggatgcgtt cgatcggata ccagcccgcg ctcccgttgc tcgaggccat cgccagcgcg
     3121 tgcgctttgc ccgcgagctc ccgcgtctgc ccgtagttga acgtgagccg cgtgttcgtg
     3181 atggtcgcca gcacgttgcc gtgaccatcg cgcaacgatg cgcccggctc gatcccccag
     3241 cggtcgagcc cactcgcagt caccacgcgc tgactcacgc cgggcgcgcg aaaccggcag
     3301 ttgaacgggc ttgccacaca ccactcgccg ttgacacccg gaaacgtcgc cgccattgcg
     3361 gccgaggcgt cgacctcgtc gatcagatcc tcatcgatca actcgtcatc gacgcacgcg
     3421 acgaacgcca gcaggcacaa gctcctcatc atgcggccgc atgttgcagc gcccgtgcct
     3481 cgcgtggttg ctcctactga gcggcctttc gtcgaagatc acacgaatgt tcgtgcaaac
     3541 gcgcgtgagc accaagcgtg ctcgtgcaaa cgcacgtgag cacg
//
LOCUS       DATLBZ010000568         8946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3379829, whole genome shotgun sequence.
ACCESSION   DATLBZ010000568 DATLBZ010000000
VERSION     DATLBZ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379829"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VMZ53_28270"
     CDS             <1..447
                     /locus_tag="VMZ53_28270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32442.1"
                     /translation="DADKKWLFVYLDTAAGGETQSEQYNTQRVTFPGTFAADYYARYK
                     VDGTFASLQHGSGGTWTTATPAPTTAQSGMFVELAIPLAAIGAGTSLDLVTYMINEKA
                     LAEGTFAGLYTGNFADGYAVNTQLTAYLHADFTSQRTPNDAANKRP"
     gene            complement(444..1340)
                     /locus_tag="VMZ53_28275"
     CDS             complement(444..1340)
                     /locus_tag="VMZ53_28275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32443.1"
                     /translation="MGRRWVVATLLPLCACGAQLGDGPGDASQNNNPDSPLTPNVDGS
                     PDGRTMLGPWGTATKVTGASDAALDEDDVVISANELELYFKKSNGTDVDLYVMTRTST
                     GMPFGAPSAITTLNSTAGEESPRLADNDLTIYFGRGGDIYKATRASVNTQTWSTPSPV
                     AAFNTGANEKWAVVCSNGYAMVSRANGTNGQDLYEGNVATTVNTLVTQLNTTTNEQGN
                     FLTSDCLTLYFQSNRTNDQYDIYESTRTTTSSAWSNPTAISDFNTATLNEEDGWLSPS
                     QRTFAYASNAANNQKDVYISTR"
     gene            1518..1958
                     /locus_tag="VMZ53_28280"
     CDS             1518..1958
                     /locus_tag="VMZ53_28280"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32444.1"
                     /translation="MKLKNTLASLAIAGSFAVMASGCVVRAHGHVGAPVAYVEVEEEP
                     PPPRYVVVDTRPGYIWVEGRWDRRGNQWVWRDGYYERERSGYVWEQGRWERRGRGHVW
                     VQGSWRAGGNGNVNNGNGQVRDHRTGGGPPPNQGGPVVRDHRTH"
     gene            1988..3151
                     /locus_tag="VMZ53_28285"
     CDS             1988..3151
                     /locus_tag="VMZ53_28285"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUS32445.1"
                     /translation="MRIALLGLFLFSACSKGTATVGQQPAKRDAQVVVADADPSKLEG
                     KALYTTLCAHCHGPNLEGYKSDNAPSLKNPTFLESATDDFLTRSINAGRPGTSMAGYG
                     HAIGGPLDDAAVARLVGYIRSQGPQAKPLPPTPSTGDPVKGQIIYARDCQKCHGDPTV
                     RMTAVHLANMRFLEMASDEFIRYAIVNGRPGTPMEAFAAKLQPVEIEHVVAYIRSFSK
                     PQQIGSLPAPTGKEPLVINPTGKEPQFKNMRSDPGQTLPRYVSVDEVKKALDEKRKLI
                     IIDARPASDWMQVHITGAVSIPYHDMKRLDDMPKDVYAIAYCACPHHLSGVVVDELLK
                     RGYKKALVLDEGILEWHRRGYPVVAAPGVMPPPKEEQIVPINPVAPGQVPKKK"
     gene            3209..6028
                     /locus_tag="VMZ53_28290"
     CDS             3209..6028
                     /locus_tag="VMZ53_28290"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HUS32446.1"
                     /translation="MNKRGGVVVVAIVLLAVIGWVGYSRYGKKTTSASSTKTGSAAAG
                     GKGAAAGQGGVNVDVTSMPMWLGQFGVAKKRIAGKVMHGDKPVAGATVRLGADLAGFA
                     LQPIAEVKSDASGAFDFGPQIAGAFDVSAESEQFTAAAKRIDLADPKAKSDKIVLQLG
                     DCRSRLFGSVLDASGGAIAKARVTTTGLAGGESDAAGKYSVCVAEGDSSARIVADGYG
                     SLDVRLHLFGELQYDFVLVPEAVLVGQVITQDKQPVAGARVVAIPDPGEGPHHLAPGW
                     TATDADGRFRIDGISPGHYRLGAAAEGLVSASPVDAIAATGTAPQDTIIVVSTVARVS
                     GRVVMGDKPVAGARVFVPKPGTIASAFTQDDGTFVMDRVPMGSVPIAVAPYEVKAPKT
                     IEVKSAKVDDITIDVSAMGSIVGKVTRHGKPVAGADVISPALRGPPVKSDENGDYALL
                     GLPPGQIDFFAWDLASRSFTPNRKEKLGPGETKHVDLELDRAGTATGVVVDDGGKPVP
                     GVYVRMISEDGTFDQGESMTDAAGKWMCHTMAGGDYIANVYPSPMAGQAFAPATGDRF
                     PAIKVPADGSVAGVTLAIKFEQLSIKGSVTDDAGSPVADVHIEAIGRGFGGMDLPSIM
                     SRADGTFEIKKLARGTYALHAHASDGSEADAQNIASGSEGVAIRLIRPGAIEGTLDGF
                     TKTPDVSMLTLTANLQIGTNAIVDGNRFYARGLRPGRYAVEAKADLESAGETVEVKSG
                     ETAKVVLKNRGTGHVEGHVAEFGTKAPLSGWQCDANLSVNGQMGQGPRDPTLTGFSDE
                     KGHFAMTAPLGKVRVFCFSPGGSHSVAGTDVDVTSQAPAVAQVFAVPNKFGATPGDPG
                     FRIRPIVLPLVVNDVVPGGPAATQGLKAGDIIVTIDGATLQGVLPMGASFLLGNHKPG
                     TTVTIGVERGGAPLTLKITLGATPT"
     gene            6097..6711
                     /locus_tag="VMZ53_28295"
     CDS             6097..6711
                     /locus_tag="VMZ53_28295"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HUS32447.1"
                     /translation="MRKVLVLFAHPVLERSRVNRRLAEAIRGIENVTIRDLYELYPTL
                     NIDVQAEQEQLLAHDVIVFQHPFYWYSCPAILKEWQDLVLEHGWAYGAGGTHLRGKIT
                     LNAVTTGGPESAYRKGGYNRFTVRELLSPWDQTAHLCGMRFLAPFAVHASLRVVGDDD
                     LKVHRTNYVRLIEALRDERIDLERAAAAENLVKLDDVLVARETA"
     gene            6708..8609
                     /locus_tag="VMZ53_28300"
     CDS             6708..8609
                     /locus_tag="VMZ53_28300"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HUS32448.1"
                     /translation="MTDSVLAQALVYLAAAVVFVPIAKRLGLGAVLGYLFAGVIIGPW
                     VLGLVGGEGHHVMHFAEFGVVMMLFLVGLELRPALLWQMRRPIFGLGGLQVAVTAAVI
                     AGGALALGVQWKSAIAVGLTFAMSSTAIVLSTLSEKGMLKTQGGQSSFSVLLFQDISV
                     IPILAVFPLLGVSVVKKVTDVTSSAGRPAWVSALLVIGAVVAVIAFGRFVVRPLFRFL
                     AGAKLREAFTAAALMIVVGIALLMQQVGLSPALGTFLAGVVLADSEYRHELEVDIEPF
                     KGLLLGLFFISVGAQIDFGLIADSPAKIAGIVVGTMAAKLAVLHTLGRLFGLDRPARW
                     LFAFGLAQVGEFAFVLLNIGVTQAVFSEAFASPLVAAVAISMVLTPPAFIFLERWVLP
                     RVTDQAPNREEDKIEDADTPVVIAGYGRFGQMVGRILRANRIPTTILDLDPEMVEIVG
                     RLGVKVYYGDASRTELLHAAGCEKAKLFVIAVDDAATATKIAANVRHHFPHLTILARA
                     RDRPHYWELRQLGVSRVFRETFGSAYETGIAALQALGYRAYTANRLARKWRDHEERVL
                     EELGGLWGKDRDAYLNRSRGAMDEAERLMRDEDPTVFEERDSAWDNESLRADRQVDAA
                     ATKPDQPSA"
BASE COUNT         1562 a         3042 c         2814 g         1528 t
ORIGIN      
        1 gacgccgaca agaagtggct gttcgtctat ctcgatacag cggcaggcgg cgagacgcag
       61 agcgagcagt acaacacgca gcgcgtgacg ttcccgggca ccttcgcggc cgactattac
      121 gctcgctaca aagtcgacgg cacgttcgca tcgctccagc atggaagcgg cggtacgtgg
      181 accaccgcaa cacccgcacc gacgaccgcg cagtccggca tgttcgtcga gctcgcgata
      241 ccgctcgccg cgatcggcgc ggggacgtcg ctcgatctcg tcacgtacat gatcaatgag
      301 aaggcgctcg ccgaaggcac gttcgccggt ctgtacaccg ggaacttcgc cgatggctac
      361 gcggtcaata cgcagctaac ggcatatctg catgcggact tcacgtcgca gcgcacgccg
      421 aatgacgccg caaacaagcg gccctagcgc gtcgagatgt agacgtcttt ttggttgttc
      481 gccgcgttgc tcgcgtatgc gaacgtgcgc tggctcggcg acagccagcc gtcttcttcg
      541 ttcagcgtcg ccgtattgaa gtcgctgatc gccgtcggat tgctccacgc cgacgacgtg
      601 gtcgtgcgcg tcgactcgta gatgtcgtac tggtcgttcg tgcgattcga ctggaagtag
      661 agcgtgaggc aatccgacgt gaggaagttg ccctgctcgt tggtcgtcgt gttgagctgc
      721 gtgacaagcg tgttcaccgt cgtcgcgacg ttgccttcgt agagatcttg gccgttcgtc
      781 ccgttcgcac ggctcaccat cgcgtagccg ttgctgcaga ccacagccca cttctcgttc
      841 gcgcccgtgt tgaacgcggc caccggcgac ggagtcgacc aggtctgcgt gttcacgctg
      901 gcgcgcgtcg ctttgtagat gtcgccaccg cggccgaagt agatcgtcag gtcgttgtct
      961 gcgagacgcg ggctctcttc gcctgccgtg ctgttgagcg tcgtgatcgc gctcggcgcg
     1021 ccgaacggca tgcccgtcga cgtgcgagtc atcacgtaga gatcgacatc cgtgccgttg
     1081 ctcttcttga agtagagctc gagctcattc gccgagatca cgacgtcgtc ttcatcgagt
     1141 gccgcatccg atgcgccggt gaccttcgtt gcggtgcccc acgggccgag catcgtgcgg
     1201 ccgtcgggcg atccgtcgac gttcggggtc agcgggctgt cggggttgtt gttctgcgag
     1261 gcatcgcccg gtccgtcacc gagttgcgcg ccgcacgcac aaagcggcag cagcgtggcg
     1321 acgacccaac gacggcccat gcttcatcat acgacacagg ctcaccaatc gattgaacga
     1381 atcactaacc gccaccgtca gtgtggtgtg gctttcacag gcctgccact tcacgttccg
     1441 acggaatcgg gcgtaatgtc gaggcaatct gcctggtacc gagtttgctc tccatactgg
     1501 gcctgaggag gatcgaaatg aaattgaaga ataccctcgc tagcctagcc atcgccggtt
     1561 cgttcgccgt gatggcatcc ggatgcgttg tacgtgctca tggtcacgtc ggcgcccctg
     1621 ttgcgtacgt cgaggtcgag gaggagccgc ctccgcctcg ctacgtcgtc gtcgacaccc
     1681 gcccgggcta catctgggtg gaaggccgct gggatcgccg cggcaatcag tgggtctggc
     1741 gcgacggcta ttacgagcgc gagcgctctg gctacgtctg ggagcaaggc cgctgggagc
     1801 gacgcggtcg cggtcacgtc tgggttcaag gctcgtggcg cgccggcgga aacgggaacg
     1861 tgaacaacgg caacggccag gttcgcgatc accgcaccgg tggtggccca ccgccgaacc
     1921 aaggtggccc cgtcgttcgc gaccaccgca cgcactagca agcgggactc gcgcatatag
     1981 tccgagcgtg cgcatcgcgc tcctcggact gtttctattt tcggcgtgca gcaaaggcac
     2041 cgcgaccgtc gggcaacagc ccgccaagcg cgacgcgcag gttgttgtcg ccgacgccga
     2101 tccatcgaag ctcgaaggca aggcgctgta caccacgctg tgcgcgcatt gccatggccc
     2161 aaacctcgag ggctacaaga gcgacaacgc gccgtcgctg aagaacccga cgtttctcga
     2221 gagtgcgacg gacgatttcc tcacgcgctc gatcaacgcc ggtcgccccg gaacctcgat
     2281 ggccggttac ggtcacgcga tcggcggccc actcgacgac gcagccgtcg cgcgtctcgt
     2341 cggctacatc cgctcgcagg gaccgcaagc gaagccactt ccgccgacgc cgtcgaccgg
     2401 cgatccagtg aaggggcaaa tcatctatgc gcgcgactgt caaaagtgcc acggcgatcc
     2461 gacggtgcgc atgacagccg tgcatctcgc gaacatgcga tttctcgaga tggcgtcgga
     2521 tgaattcatt cgctacgcga tcgtgaacgg tcggcccggc acgccgatgg aagcgttcgc
     2581 agcaaagctc cagcccgtcg agatcgagca cgtcgtcgcg tatatccgca gcttcagcaa
     2641 gccgcagcag atcggttcgc tgccggcacc gacgggcaaa gagccgctcg tgatcaatcc
     2701 aaccggcaag gagccgcagt tcaagaacat gcgctcggac cctggccaga cgctgccgcg
     2761 ttacgtgtcc gtcgacgaag tgaagaaggc gctcgacgag aagcgcaagc tcatcattat
     2821 cgacgcgcgc cctgcgtcgg actggatgca agtccacatc accggcgcgg tctcgatccc
     2881 gtatcacgac atgaagcgcc tcgatgacat gccgaaagac gtctacgcga tcgcgtactg
     2941 cgcgtgcccg catcacttga gcggcgtcgt cgtcgacgag ctcctcaagc gcggctacaa
     3001 gaaagcgctc gttctcgacg agggcatcct cgagtggcat cgccggggct atcccgtcgt
     3061 cgcagcgccc ggcgtcatgc cgccgccgaa agaggagcag atcgttccga tcaatccggt
     3121 ggcacccgga caggtaccga agaaaaagta gcgctgcgac gccctgcgac agcaacggcg
     3181 cgctgatcgg cgggtctaag atgccgtgat gaacaaacgc gggggcgttg tcgttgtcgc
     3241 gatcgtcctg ctcgccgtga tcggctgggt cggctattcg cgctacggga agaagacgac
     3301 cagtgcgtcg tcgacgaaga ccggctcggc cgcggcgggc ggtaaaggcg cggctgcggg
     3361 gcagggcggc gtgaatgtcg atgtgacgtc gatgccgatg tggctcggcc agttcggggt
     3421 cgcgaagaag cgcatcgccg gcaaggtgat gcacggtgac aagcccgttg cgggcgcgac
     3481 cgtccggctc ggagcagacc tcgccggctt cgcgctgcaa ccgattgcag aagtgaagag
     3541 cgacgcgagc ggcgcgttcg acttcgggcc gcagatcgcc ggcgcattcg acgtcagcgc
     3601 cgagtccgag caattcacgg ccgccgccaa gcgcatcgac ctcgccgatc cgaaagcgaa
     3661 gtcggacaag atcgtcctgc agctcggcga ttgccgctcg cgcttgttcg gctccgtgct
     3721 cgacgcatcg ggcggcgcga tcgcaaaggc gcgcgtcacg acgaccggcc tcgctggcgg
     3781 cgagagcgac gcggctggca agtacagcgt ctgcgtcgcc gagggagact cgagcgcgcg
     3841 aatcgtcgcg gacggctacg gctcgctcga cgttcgcctg catttgttcg gcgagctcca
     3901 gtacgacttc gtgctcgtgc ccgaggccgt cctcgttggc caagtcatca cacaggacaa
     3961 gcagccggtc gctggggcac gcgtcgtcgc gattcccgat ccaggcgaag gcccacatca
     4021 cctcgcgccg ggctggaccg cgaccgatgc cgacggccgc tttcgcatcg acggcatctc
     4081 gcccggccac tatcgccttg gcgccgccgc agaaggactc gtttcggcat cacccgtcga
     4141 tgcgatcgcc gcaacgggca ccgctcctca ggacacgatc atcgtcgttt cgaccgtcgc
     4201 tcgcgtcagt gggcgcgtcg tgatgggaga caagcccgtc gcgggagcac gcgtattcgt
     4261 accgaagccg ggaacgatcg cgagcgcgtt cacccaagac gacggaacgt tcgtgatgga
     4321 tcgcgtaccg atgggcagcg tgccaatcgc cgttgcgccg tacgaggtta aagcgccgaa
     4381 gacgatcgag gtgaagagcg cgaaggtcga cgacatcacg atcgacgtca gcgcgatggg
     4441 atcgatcgtc ggtaaggtca cgcgccacgg taagcccgtc gcgggtgccg acgtcatcag
     4501 ccccgcgctg cgtggtccac ccgtgaagag cgacgagaac ggcgactacg cgctcctcgg
     4561 tctgccgcct ggacagatcg atttcttcgc gtgggacctc gcctcgcgct cgttcactcc
     4621 gaatcgcaaa gagaagctcg gcccgggcga gacgaagcac gtcgacctcg agctcgatcg
     4681 cgcaggcacc gcaaccggcg ttgtcgtcga cgacggcggc aagcccgtgc ccggcgtcta
     4741 cgtgcgcatg atcagcgaag acggcacgtt cgatcagggc gaatcgatga ccgacgcggc
     4801 tgggaagtgg atgtgtcaca cgatggcggg cggcgattac atcgccaacg tctatccatc
     4861 cccgatggca ggccaagcct tcgcgcccgc aaccggcgat cgctttcccg cgatcaaagt
     4921 tccggcggat ggctccgtcg ccggagtcac actcgcaatc aagttcgaac aactctcgat
     4981 caaaggcagc gtcaccgacg acgccggctc gccggtcgct gacgttcaca tcgaggcgat
     5041 cggccgcggc ttcggcggca tggacctgcc ctcgatcatg agccgcgccg acggcacgtt
     5101 cgagatcaag aagctcgcgc gcggcacgta cgcactgcac gcgcacgcct ccgacggcag
     5161 cgaggccgat gctcagaaca ttgcgtcggg cagcgaaggc gtcgccatcc ggctgattcg
     5221 gcccggggcg atcgagggca cgctcgacgg cttcacgaag acgccggacg tctcgatgct
     5281 cacgctcacc gcgaatctcc agatcggaac gaacgcgatc gtcgacggca atcggttcta
     5341 cgcacgcggg ttgcgaccag gccgttacgc cgtcgaagcg aaagccgacc tcgagagcgc
     5401 gggcgagacc gtcgaggtga agtccggcga gaccgcgaag gtcgtgctga agaatcgcgg
     5461 caccggccac gtcgaaggcc acgtcgccga attcggcacg aaggctcctc tctccggctg
     5521 gcagtgcgac gcgaacctgt ccgtcaacgg ccagatgggg cagggcccgc gcgatccgac
     5581 gctcacaggc ttctccgacg agaagggcca cttcgcgatg accgcacctc tcggcaaagt
     5641 ccgcgtgttc tgcttctcgc cgggcggcag tcacagcgtc gcgggcaccg acgtcgacgt
     5701 cacgagccaa gcgcctgcgg tcgcgcaggt cttcgccgtc ccgaacaagt tcggcgcgac
     5761 gccgggcgat cccggattcc gcatccgacc gatcgtgctg ccgctcgtcg tcaatgacgt
     5821 cgtgcccggc ggcccggccg ccacgcaagg cctcaaagcg ggcgacatca tcgtcacgat
     5881 cgacggcgca acgctgcaag gtgttctgcc aatgggcgcg agctttctcc tcggcaatca
     5941 caagcccggc accaccgtca cgatcggcgt cgagcgcggt ggcgcaccgc tgacgctgaa
     6001 aatcacgctc ggcgcaacgc cgacctgacc gctcgggcag tcagatcatc cggctcacgc
     6061 gcgcacgtcc gcgcgcaatt cggcgagaat cgcgcaatgc gcaaggtcct cgtcttgttc
     6121 gcgcatccag tgctcgaacg atcgcgcgtc aatcgccgcc tcgccgaggc catccgcggc
     6181 atcgagaacg tcacgattcg cgatctctac gagctctatc caacgctcaa catcgacgtg
     6241 caggccgaac aagagcagct cctcgcgcac gacgtgatcg tcttccagca tccgttctac
     6301 tggtactcgt gccccgcgat cctcaaggag tggcaagacc tcgtcctcga gcacggctgg
     6361 gcctacggcg caggcggcac gcacctgcgc ggcaagatca cgctcaacgc cgtcacgacc
     6421 ggcggtcccg agagcgcgta tcgcaaaggc ggctacaacc gcttcaccgt tcgcgagctc
     6481 ctctctccgt gggatcaaac cgcccacctc tgcggcatgc gctttctcgc gccattcgcc
     6541 gtgcacgcat cactccgcgt cgtcggcgac gacgacctca aggtgcatcg caccaattac
     6601 gtgcgcctca tcgaagcact gcgtgacgag cggatcgatc tcgagcgcgc ggccgccgcc
     6661 gaaaacctcg tcaagctcga cgacgtcctc gtcgcacggg agactgcatg accgacagcg
     6721 ttctcgcgca agcgctcgtc tatctcgccg ccgcggtcgt gttcgtgcca atcgcgaagc
     6781 ggctcggcct cggcgccgtg ctcggctacc tattcgccgg cgtcatcatc ggaccgtggg
     6841 tgctcggcct cgtcggcggt gaaggtcacc acgtgatgca cttcgccgag ttcggcgtcg
     6901 tgatgatgct gttcctcgtc ggtctcgagc tcaggcccgc gctgctctgg cagatgcgac
     6961 gcccgatctt cggcctcggt ggcctacagg tcgcggtcac ggccgccgtc atcgccggcg
     7021 gcgcgctcgc gctcggcgtt cagtggaagt ccgcgatcgc ggtcggcctg accttcgcga
     7081 tgtcctcgac cgcgatcgtc ctctcgacgc tcagcgagaa aggcatgctc aagacgcaag
     7141 gcggccagtc gagcttctcc gtcctcctct tccaggacat ctccgtcatt ccgatcctgg
     7201 ccgtcttccc gctcctcggc gtctcggtcg tcaagaaggt caccgacgtc acgtcgagcg
     7261 ccggccgtcc agcatgggtc tccgccctcc tcgtcatcgg cgccgtcgtc gcggtgatcg
     7321 cattcggtcg cttcgtcgtc cgaccactct tccgcttcct cgccggtgcg aagcttcgcg
     7381 aagccttcac cgccgccgcc ctcatgatcg tcgtcggcat cgcgctgctc atgcagcaag
     7441 tcggcctctc accagccctc ggcacgttcc tcgccggcgt cgtcctcgcc gacagcgaat
     7501 accgccacga gctcgaggtc gacatcgagc cgttcaaggg cctgctcctc ggcctcttct
     7561 tcatttccgt cggcgcacag atcgacttcg gtttgatcgc cgattctcct gccaagatcg
     7621 cgggaatcgt cgtcggaacg atggccgcga agctcgccgt cctccacacc ctcggccgcc
     7681 tgttcggcct cgaccgccca gcgcgctggc tcttcgcatt cggcctcgct caagtcggcg
     7741 agttcgcgtt cgtcctcctc aacatcggcg tcacgcaagc cgtcttcagc gaagcattcg
     7801 cgagcccact cgtcgccgcc gtcgcgatct cgatggtgct caccccaccc gcgttcattt
     7861 tcctcgagcg ctgggtcctc ccgcgcgtaa ccgatcaagc gcccaaccgc gaggaagaca
     7921 agatcgaaga cgccgacacc ccggtcgtca tcgcaggcta cggccgcttc ggccaaatgg
     7981 taggccgcat cctccgcgca aaccgaatcc caaccacgat cctcgatctc gatcccgaga
     8041 tggtcgaaat cgtcggacgc ctaggcgtca aggtctacta cggcgacgca tcgcgcacgg
     8101 agctcctcca cgcagccggc tgcgagaagg cgaagctctt cgtcatcgcc gtcgatgacg
     8161 cggccaccgc aaccaagatc gccgcgaacg tgcggcacca cttcccgcat ctcacgatcc
     8221 tcgcccgcgc tcgcgaccgc ccgcactact gggagctacg acagctcggc gtatcgcgcg
     8281 tcttccgcga gaccttcggc tcggcgtacg agacgggcat cgccgcgctg caagcgctcg
     8341 gctatcgcgc gtacaccgcc aaccgcctcg cccgcaaatg gcgcgaccac gaagagcgcg
     8401 tgctcgaaga gctcggcggc ctctggggca aagatcgcga cgcgtacttg aatcgctcgc
     8461 gcggcgcgat ggacgaagcc gaacgcctca tgcgcgacga agatccgacg gtgttcgagg
     8521 agcgcgactc ggcgtgggac aacgagtcgc tgcgcgccga tcgccaagtc gacgccgccg
     8581 cgacgaagcc cgaccagcca agcgcgtagg tgcgcgtcga agcgtgccga cgcgggtcgt
     8641 gatggatcga tgccgatgcc cacgtggggc gtaatcccgc gttcgctacc tggtcgcgag
     8701 ctcgatgctg tcgtgggcgt gcgatgcggt gcccacctgg gtcgtaatcc cgcgttcgct
     8761 accgggtcgc gagctcgatg cggtcgtggg cgcgcgaagc gatgcccacg tgggacgtga
     8821 tctccgcaat cggtgcctga ttgttagctc gacgccatcg aggcgtgcga ggcgatgccc
     8881 acgtgggtcg tggatccatc ggtgctcgcg tgttctattt cacttggcga agagcgccgg
     8941 ccggtg
//
LOCUS       DATLBZ010000569         3654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10067578, whole genome shotgun sequence.
ACCESSION   DATLBZ010000569 DATLBZ010000000
VERSION     DATLBZ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10067578"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VMZ53_28305"
     CDS             complement(<1..1013)
                     /locus_tag="VMZ53_28305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32449.1"
                     /translation="MLRDGRAEPPTLTRTEKAEPHRDDILALYAECGGNLVRVHEELS
                     ARHQLALSYPALTAFCRKHGIGHEPKKPAGQYHFDPGTEMQHDTSPHDAHIGGALRRV
                     QTASLVLCYSRRLFFQFYPRFTRFECKTFLDDGVGYHDGACEHCMIDNTHVVVLHGTG
                     KTMVPVPEMEAFARRLRFTFIAHEKGDANRSARVERPFHFIEKNFLAGRKFADFADAN
                     RQAREWCDKINAKFRRSLSSTSNELYAVERLAMRRLPLWRPEIYLLHQRVVDLEGYIH
                     VDGHIYSVPYQLIGKAVEVRETKDKVRVFAGPREVAVHDKVISTTAKHRRTLAEHRPP
                     RGQ"
     gene            complement(1078..1953)
                     /locus_tag="VMZ53_28310"
     CDS             complement(1078..1953)
                     /locus_tag="VMZ53_28310"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HUS32450.1"
                     /translation="MDVDLHQLELRHHDLRIHDAEQRRRLIGSVAEIGQQVPVIVIRE
                     DQRLVLIDGYLRVEALRKLGRDTAAATAWPVDEVEALLHRRHLCIAVPAIEDAWLLAR
                     LRDHGLALDDLARRLCRSKSWVSRRLALIEALTPAAQARVRAGTVPPHAAMKYLVPLA
                     RANKRQCEQLVTGLGETRVSDRELGALYDAWRCADRVGKQRIVAEPLLVLRALAATST
                     ADIEDESAPLHKDFTMLAAISWRASRRVRGGDRLTAELTRAWRAAREAFASLGTTIEE
                     MHAGPDDTTNHSETS"
     gene            complement(2530..3042)
                     /locus_tag="VMZ53_28315"
     CDS             complement(2530..3042)
                     /locus_tag="VMZ53_28315"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS32451.1"
                     /translation="MCRMFGVSRPTGYTWIERYREANNDLTAVNEKSRRPKTSPTAIS
                     PELEDLIVEMRKRHPRWGPRKLHAILVQRNPSAYVPSASVMAKVLKRRGMTTIRRRRR
                     SVAMNITPPFKGCTSSNDVWCIDFKGWFVTQDQVKCYPLMLIDAFSRYLLRCEALTEP
                     DGKHFDACQN"
BASE COUNT          630 a         1154 c         1181 g          689 t
ORIGIN      
        1 tgttgtccgc gcggcggacg atgctctgcc aacgtgcggc ggtgcttggc cgtcgtcgag
       61 atcaccttgt cgtgaacggc gacctcgcgc ggcccagcga agacgcgcac cttgtccttg
      121 gtctcgcgaa cctcgacagc cttgccgatc agctggtagg gcaccgagta gatgtggcca
      181 tcgacgtgga tgtagccctc gaggtcgacg acgcgctgat gcagcagata gatttcaggt
      241 cgccacagag gaagccgacg catggcaagc cgctcgacgg cgtagagctc attggaagtc
      301 gacgacaggc tccggcggaa ctttgcattg atcttgtcgc accactcgcg cgcctggcga
      361 ttggcgtcag caaagtcagc gaacttgcgg ccagcgagaa aattcttctc gatgaaatga
      421 aacggccgct cgacgcgcgc cgagcgattg gcgtcgccct tctcatgcgc gatgaacgtg
      481 aacctcaacc ggcgcgcgaa tgcttccatt tccggaaccg gcaccatcgt cttgcccgtg
      541 ccgtggagca cgacgacgtg ggtgttatcg atcatgcagt gctcgcacgc gccgtcgtgg
      601 tagccgacgc cgtcgtcgag gaacgtcttg cattcgaagc gtgtgaaccg cgggtagaac
      661 tggaagaaca gccggcgcga gtagcacagc accagcgagg cggtttgcac gcgacgtagc
      721 gcaccaccga tgtgtgcgtc gtgcggcgag gtgtcgtgct gcatctccgt gccgggatcg
      781 aagtggtact gcccagccgg cttcttcggc tcgtggccga tgccgtgctt gcggcagaac
      841 gcggtcagcg ccggatacga gagcgcgagt tggtgacgag cggagagctc ctcatgaacg
      901 cggacgagat tgccgccgca ctcggcgtag agcgcgagaa tgtcatcgcg gtgcggctcc
      961 gccttctcgg tgcgcgtcag cgttgggggc tctgcgcggc cgtcgcggag cacgcgcttg
     1021 accgcacccc gtgagatctg gagtgcgtcg gcaatcgcgc gcgagccgtg gcccgcttca
     1081 tgaagtttca gaatggtttg tcgtgtcgtc tggtccagca tgcatctcct cgatggtggt
     1141 gccgagcgac gcgaacgctt cgcgtgcggc gcgccaggca cgagtgagct cggcagtgag
     1201 ccgatcgccg ccgcgaacac ggcgactcgc tcgccacgag atcgcggcga gcatcgtgaa
     1261 gtctttgtgg agaggcgctg actcatcctc gatgtcagcc gtcgacgtcg cggcgagagc
     1321 gcgcaacacg agcagaggct cggcgacgat ccgctgcttg ccgacgcggt cggcgcatcg
     1381 ccatgcgtcg tagagcgcac caagctcgcg atcactgacg cgcgtctcgc ccagcccggt
     1441 caccagctgc tcgcattgcc gcttgttggc gcgcgccaac ggcacgaggt acttcatcgc
     1501 tgcgtgtggc ggcaccgtgc cggcgcgaac acgcgcttga gcggctggag tgagcgcctc
     1561 gatcaacgcg agtcgtcgcg acacccagct cttcgatcgg cagagtcgtc gcgcgaggtc
     1621 atcgagcgcg agtccatggt cacgcaatcg cgcgagcagc cacgcgtcct cgatcgcggg
     1681 gacagcgatg cagaggtggc gacgatgcag gagtgcctcg acctcgtcca ccggccacgc
     1741 cgtcgccgcg gcggtgtcgc ggccgagttt gcgtagcgcc tcgacgcgga ggtagccgtc
     1801 aatcagcacc aaccgctggt cttcgcggat cacgatgacc ggcacttgct gcccgatctc
     1861 ggcgaccgat ccaatcagtc gccgtcgctg ctcggcgtcg tggatccgga gatcgtgatg
     1921 acgaagctcg agctggtgca ggtccacgtc cacgcgtgca cgctgcaccg acgtggactt
     1981 agggccgagg cctgcccgtg acatcgtgcg ccaggatcac ggcaaaaacc tcagctacct
     2041 ccaacgctta gccggcgagg cctgcacgtg acagcgactc ttcggggtcc tgttttgcgg
     2101 tcacggaacg aggcgaccgc cgcagaatca caccgagttt ccgctcgagg cctgcgcgtg
     2161 acatcgcgat cgcgctgtcg aacgattcga gcattttctc gaccaattcc gtcggcttcg
     2221 gctctgaggc ctgcacgtga cagccggagg tctccgcggc tcgccgcgat cgcgatgcct
     2281 gctgccgctc gcgctcgtca acgcgcgcat cctgcacgcg tcgcgctcgt cgtcgacgcg
     2341 cgagcttttg cttccgggct tgacggcagt cctcgctgca cgtccgctga tggtccgcgg
     2401 cagacggtac cggccggaac gaggctctgc actcagtgca acatcggcgg gtgcgcagcg
     2461 gcatcgagcc acgcgtaccg caccgtcatc gccgatcgtg gtgggtcaat tctcgcgagc
     2521 aacaccgggt caattctgac aagcgtcgaa gtgtttcccg tctggctcgg tgagcgcctc
     2581 acaacgcaga aggtatcgag agaacgcgtc gatgagcatg agcgggtagc acttcacttg
     2641 gtcttgcgtg acgaaccagc ccttgaagtc gatgcaccag acgtcgttcg acgaggtgca
     2701 gcctttgaac ggcggcgtga tgttcattgc caccgaacgt cgtcggcgcc gtatggtcgt
     2761 catgccgcgc cgcttgagaa cctttgccat cacactcgca ctcggcacgt atgcgctggg
     2821 gtttcgttgg acgaggatcg cgtgcagctt tcgaggtccc catcgcggat gtcgctttcg
     2881 catctcgacg atcagatctt cgagctctgg tgaaatcgcc gtcggcgacg tcttcggtcg
     2941 acgagacttt tcgttgaccg ccgtcagatc gttgttggct tcgcggtatc gctcgatcca
     3001 cgtgtagccc gttggtcgac tgactccgaa catccggcac agctcacgat gttcacgtgt
     3061 ccgccttcag cttccgtaaa cctgcgctcc cactccaaga cgagcttcgt ccgctccttc
     3121 atcgtgtccg tcgctttcca gggcatgccc gcgacggtcg ccgtcacctt cgacatcaac
     3181 gtgtaaacga tgtccgtggg catcggatgt caactgtcag ggatgtcgtg aggtagtcct
     3241 gtaaaggatc taggtgatct gcaccccacc ggatctccaa cctggaaatc gctgaaagta
     3301 cttctagtat aaacatcggc gaaaatcatt gcggtccaga tgatggcgtg gcacgttccg
     3361 tgtgtggggc ggtatcggag gacacacgag caactcgagc tggcgcttcg tcgcgacaag
     3421 aacggcggcc gtcgtggcgg ccgccggcca ggtgcaggtc gcccacggaa gtcgcgagcg
     3481 ggggcagcac acaagacccg accgatattg cgacggcgtt ttcccgttca cgtcgtcctt
     3541 cgcgtgcatg gcgacatcag cagcctgcgc aaacggcaca tgtatcgtgc gctgcgcgag
     3601 gcgacggcga cggtgctgcg acgggagctg gactttgcca agttcggcgc gttc
//
LOCUS       DATLBZ010000570         3053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7096184, whole genome shotgun sequence.
ACCESSION   DATLBZ010000570 DATLBZ010000000
VERSION     DATLBZ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7096184"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VMZ53_28320"
     CDS             complement(<1..585)
                     /locus_tag="VMZ53_28320"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32452.1"
                     /translation="MQLLPTTMVGSYPRPLWFTQQLAGRDVLEAFKVASHAEAFHDAT
                     RVVIKDQEEAGLDILTDGQMWFDDYHMGIGSFLWYWLERTKGFSAEKLPHPARAKAKG
                     RDIWALDEAGGVAVEGAIERGPVRMAAIYQWAQRHTQKPIKACIGAGPVQLSTLAHFR
                     SGPVKNRYDLSRALADVFRAEIGEVVQAGCKHVQL"
     gene            645..1622
                     /locus_tag="VMZ53_28325"
     CDS             645..1622
                     /locus_tag="VMZ53_28325"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS32453.1"
                     /translation="MPARCVLVLPEGPSRRVGPSGILIGRQGDCDIVASDQSVSRRHA
                     HVRLTQDGAEVVPLGRAPIDVNGVPHTRPRALSDGDELRLPGLVLKVVLSPEPAAVER
                     ATGFVLERAGGGNFGVVSSPFAIGGGSSADLIIASWPDPMFVMHVAQGELFVQALAEG
                     ATLNAQELDVDEPTTITTGDLLAYRDETFEVKYFAGFQTTTAVKRADLPVKVVIEMLP
                     RGGRITFTSGDGEHAVNLADRRLDLLIALLRPPEGYKAGDWIPDDVVRTIVWPRNPGV
                     SRPEINTLISRCRRDLVQAGLAGPRLIERAPTGGGTRIALAPNAQIEAL"
     gene            complement(1638..2051)
                     /locus_tag="VMZ53_28330"
     CDS             complement(1638..2051)
                     /locus_tag="VMZ53_28330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32454.1"
                     /translation="MRQWLLGTFLGVAACSASAQRPAKAPQPVVAQAPVHADTCPLQL
                     DGASLEVKELDQGVALEFTSFGDVAELRRQVRLLGDIRHAENRRFLEDVEGGARIVFP
                     AEYKAQVVALGEQMEAGACVTSLPTKPDEQIVVSR"
     gene            complement(2156..>3053)
                     /locus_tag="VMZ53_28335"
     CDS             complement(2156..>3053)
                     /locus_tag="VMZ53_28335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HUS32455.1"
                     /translation="YVTLDHDGSLLAANAVSEDKLLAGLVPAEMYPDAPQAALEAQAI
                     AARTELLQKIGRRNLTDPFLLCSTQQCQVYAGAGKEDPRTTKAIEKTRGIVLLRDGGG
                     MVDIRYSATCGGHSEDNEAIWGGEPDPSLRGHRDDSKGGITRVTDDNIGAFLDEDPKK
                     FWCGKVEKAKTKFRWTEKIAADDLTVRVSTEYPEIGRIKDIEPKQRGVSGRIEVLTIK
                     GDKASVDVTGDLHIRRLLGGLKSTLFEVKRDGKTFVFHGGGFGHGVGMCQLGAIGMAD
                     AGKAHKDILGHYYRGTHLHKLY"
BASE COUNT          497 a          976 c         1025 g          555 t
ORIGIN      
        1 gagctggacg tgcttgcagc ctgcctgcac gacctcgccg atttcagcgc ggaacacgtc
       61 ggcgagcgcg cgagagagat cgtagcgatt cttcaccggc ccggagcgaa agtgcgcgag
      121 cgtcgagagc tggacgggac ctgcgccgat gcaggctttg atcggcttct gcgtgtggcg
      181 ctgtgcccat tggtaaatcg ccgccatgcg tacggggccg cgctcgatcg cgccttcgac
      241 cgcaacgccg cctgcttcgt cgagcgccca gatgtcgcga cccttcgctt tcgcgcgcgc
      301 gggatgcggc agcttttcgg cggagaagcc cttcgtgcgc tcgagccagt accagaggaa
      361 tgagccgatg cccatgtggt agtcgtcgaa ccacatctga ccgtcggtga ggatgtcgag
      421 ccccgcttct tcctgatctt tgatgacgac gcgcgtcgca tcgtgaaacg cctcggcatg
      481 cgaagcgacc ttgaacgctt cgagcacgtc gcgcccggcg agctgctgcg tgaaccacag
      541 aggccgcggg tacgaaccca ccatcgtcgt cggaaggagc tgcatgtcgt cgagatacta
      601 cgcccgagtg tcacccgggt gttacgtgca ggtatcatcg gcccatgccc gcgcgctgcg
      661 tgctcgtgtt gccggagggg ccgtcgcggc gtgtcgggcc tagcggcatt ctgatcgggc
      721 gacagggcga ttgcgacatc gtcgcgtctg atcagagcgt tagccgtcgc cacgcacacg
      781 tccggctgac gcaagacggc gcggaggtgg tgccgctcgg acgcgcgccg atcgacgtca
      841 acggtgtgcc gcatacgcgg ccgcgcgcgc tgagcgatgg cgacgagcta aggttgcccg
      901 ggctcgtgct gaaggtcgtg ctctcgccgg aacctgccgc ggtcgagcgc gcgacgggat
      961 tcgtgctcga gcgagcgggt ggcggaaact ttggcgtcgt gtcgtcgccg tttgcgatcg
     1021 gcggcggcag cagcgccgac ctcattatcg cgagctggcc cgatccgatg ttcgtgatgc
     1081 acgtcgcgca gggcgagctg ttcgtgcaag cactcgcgga aggcgcgacg ctcaacgcgc
     1141 aagagctcga tgtcgacgag ccgacgacga tcaccacagg cgacttgctc gcgtatcgag
     1201 acgagacgtt cgaggtgaag tacttcgccg gatttcagac gacgaccgcg gtcaagcgcg
     1261 cggacttgcc ggtgaaggtg gtgatcgaaa tgctgccgcg cggaggtcgc atcacgttca
     1321 cgtcgggcga tggcgagcac gccgtcaatc tcgcggatcg ccgcctcgat ctgctgatcg
     1381 cgctgttgcg accacctgag ggctacaaag ccggtgactg gattccagac gacgtcgtgc
     1441 gaacgatcgt gtggccgcgc aatccgggcg tctcgcgtcc cgagatcaac acgctgatca
     1501 gccgatgtcg gcgcgacctc gtgcaagcgg ggctcgcggg gccacggctc atcgagcgcg
     1561 caccgacggg cggtggcaca cggatcgcgc tcgcgccgaa cgcgcagatc gaagctctct
     1621 agcggctttg taggtcgcta acgcgagacg acgatctgct cgtcgggttt cgtcggaagt
     1681 gaggtcacgc aggcgcccgc ttccatctgt tcgccgagcg caacgacctg cgccttgtac
     1741 tccgcaggga acacgatgcg cgcgccgcct tcgacgtcct cgaggaagcg ccgattctcg
     1801 gcgtgtcgga tgtcaccgag gaggcgaacc tggcgccgca gctcggcgac atcgccgaag
     1861 ctcgtgaact cgagagcaac gccttggtcg agctctttga cttcgagcga cgctccgtcg
     1921 agctggagcg ggcaggtatc tgcgtgaacc ggtgcttgcg cgacgacggg ttgcggcgcc
     1981 tttgcagggc gctgcgcgct cgcggaacaa gctgcgactc cgagaaaggt gccgagcagc
     2041 cattggcgca tgaccttcgc tcacagcaag tctgataccg ctgcaagtgg gcatcacgcc
     2101 gagggtggcg cgcgtcgtgc gacaaacact tcgacgacag cgagcgccga cgtcgctagt
     2161 agagcttgtg gaggtgcgtg cctcggtagt agtggccgag gatgtccttg tgcgctttgc
     2221 ccgcgtcggc cattccgatt gcgccgagct gacacattcc cacgccgtgg ccgaagcccc
     2281 cgccgtgaaa tacgaacgtc ttgccgtcgc gcttcacctc gaacagcgtg gacttgaggc
     2341 cgccgagcaa gcgccggatg tggagatcgc ctgtgacatc gacgctcgcc ttgtcgccct
     2401 tgatcgtgag gacctcgata cgtcccgaca cgccgcgttg cttcggctcg atgtccttga
     2461 tgcggccgat ctccggatac tcggtcgaca cgcgcaccgt tagatcgtcc gcagcgatct
     2521 tctcggtcca acggaacttc gtcttcgcct tctcgacctt gccacaccag aacttcttcg
     2581 gatcctcgtc gagaaaggca ccgatgttgt cgtcggtgac gcgcgtgatg ccgcccttgc
     2641 tgtcatcgcg atggccgcgt agcgaaggat ccggctcgcc gccccagatc gcttcgttgt
     2701 cttcgctgtg accgccgcac gtcgcgctgt agcggatgtc gaccatgccg ccgccgtcgc
     2761 gcagcagcac gatgccgcgg gtcttctcga tagctttcgt cgtgcgcgga tcctccttgc
     2821 cggcgccggc gtagacctgg cactgctgcg tcgagcacag caagaacgga tcggtcaggt
     2881 tgcggcggcc gatcttctgc agcagctctg tacgagcggc gatcgcctgc gcttcgagag
     2941 cggcctgcgg tgcatcggga tacatctcgg caggcacgag gccggcgagc agcttgtcct
     3001 cggagacggc gttcgccgcg aggagcgagc catcgtgatc gagcgtgacg tac
//
LOCUS       DATLBZ010000571         9294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8588737, whole genome shotgun sequence.
ACCESSION   DATLBZ010000571 DATLBZ010000000
VERSION     DATLBZ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8588737"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VMZ53_28340"
     CDS             complement(<1..640)
                     /locus_tag="VMZ53_28340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32456.1"
                     /translation="MRIAVVGLVAAVVGLASSRVSHAKPGAIVVPPFEAEIGAASAVG
                     ADDNTIGTATEFRIGFHWASLSWRPTPIDFGIGFVTTSRESFGGSIDRVATGPEDHSL
                     SLKGAYVSGAYAIENHKHWRTWLGARLEMLSGSNQGERVTTTGVAVRLATELYSASAG
                     GVADSHVAAFFAGAWALGVYVEGIHRGIPGDLAEVDAAAGLTMRIPFIAGVAD"
     gene            759..1184
                     /locus_tag="VMZ53_28345"
     CDS             759..1184
                     /locus_tag="VMZ53_28345"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS32457.1"
                     /translation="MPALRYNASAMGVFHHLSIGVADLARSAAFYDAALAPLGLVRIF
                     ENPRAVCYGPPGFEGEAPFALLGKGADAKPPGIGFHLAFTAPSHEAVHRFHEAAVAAG
                     GVDEGPPGTRENYSPDYYTAFVRDLDGYRLEAVVYKEPA"
     gene            1181..1630
                     /locus_tag="VMZ53_28350"
     CDS             1181..1630
                     /locus_tag="VMZ53_28350"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HUS32458.1"
                     /translation="MKVSRVIAAPRSRVFAAWMSKDAVAEWLPPAGMSARIDHFDARI
                     GGGYRMTLTYDGSGHGKTTTDTDVVNVTFDEIVLDERIVQATTFDTKDAAFAGTMTLT
                     WTFADAEPGGGTRVTVEVANAPRGITDADHEKGISSTLDNLATYVTR"
     gene            1647..2567
                     /locus_tag="VMZ53_28355"
     CDS             1647..2567
                     /locus_tag="VMZ53_28355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32459.1"
                     /translation="MAANRMLIVVALSSACGRFSFDERELDAKQDIDAVARCTAFGPW
                     SKPEMPQDISSDAEEYNGTIESSGTTIIFPSGRAGGMGSADLYMATRPSTSEPFATPV
                     NLANVNSVAADMAPTLSNDELTLYFTSNRGGGPFRLYEATRASRALPFGVPSLVPGPF
                     TGLGEVGGPDISRDGLALYFDATAGGTGGYDLWVATRASVGDPWGTPSEVSGVNTTGS
                     ENEPTIDDDGLTIYYASDAFEPAQDLVVATRPDLASPFSTPTKVPLSLNPGVGEYGAD
                     ISADGLWMELSIHLGAPMNTNVWLSSRVCL"
     gene            complement(2564..3208)
                     /locus_tag="VMZ53_28360"
     CDS             complement(2564..3208)
                     /locus_tag="VMZ53_28360"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS32460.1"
                     /translation="MGDLAERRSALRVPVRGVAVFYTEDGATHGTIENLSTSGALLTV
                     MGVPSSENDLDLELKLGDETGWVSAHTVRIERYSPTRLRIAVTFDRLEDNVRAAIDTA
                     IEHAVRAAQRRPVLVVDDKRSRRVELANRLAARGMTPLVPRTPLEAIDLLTRTQLHVA
                     VAILAATSELHGVLAESFPWVSTAQLSDDIDATVESAIDAWSTTDGARLASAIA"
     gene            complement(3353..4192)
                     /locus_tag="VMZ53_28365"
     CDS             complement(3353..4192)
                     /locus_tag="VMZ53_28365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32461.1"
                     /translation="MSEALLPVTPAAPFADPCAVVDTINDDSASYAAALADALRKKRG
                     PDIPSEIVDAQDSLERRIRAHLAKSRVAVTLTDNRYTMISVRRLAKEKRYEVRLHHMF
                     ADADPVITRALARYIADNDAEASRVLGDFIDQNTSHVRGRARRQPTQVIFTAGEHHDL
                     REIYDDLNARYFDNKIDAAITWGQRTGRPRRRNSIKMGSYSVEDRLIRIHRSLDRSFV
                     PRFFVAWIVFHEMLHQVHDIRVKNGRREFHSKEFLADEAMYEHYDEAKLWERRHLDAL
                     LTY"
     gene            4463..6250
                     /gene="aspS"
                     /locus_tag="VMZ53_28370"
     CDS             4463..6250
                     /gene="aspS"
                     /locus_tag="VMZ53_28370"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HUS32462.1"
                     /translation="MSQFLATNTRTHSCGQLRAGDVGKRVVLTGWVKTYRDHGKAVFI
                     DLRDRDGLTQLVFDESFSKQATQLANELRSEWCIGVVGEVRSRGSNVNEKLPTGSIEV
                     WVETLEVFSKAETPPFQIEDQVDTNDQVRGKYRYLDLRRPKLQKNLMMRSAITRVTRD
                     FLGKNGFLEIETPFMVKYTPGGARNFLVPSRLNPGQFYALAESPQIFKQLLMVAGYER
                     YFQIVRCFRDEDLRLDRQPEFTQIDIEMSFVNEQILQSTMEGLISTLWREVLGVELQL
                     PLRRMPYAEAMEKYGVDKPDLRCELQLCEVTDVARTCGFRVFQDVVEKGGIVKCLRVP
                     GPADKLPRSILDGLNDFAKSFNAKAAWARVQEGGQWQGIKGLTDESRAEINRIAGATP
                     GDTLIFVADKAKVANATLGAIRIHYIGEKLGLIRKGEWQFMWLTDPPLFEIEDGEVAA
                     AHHPFTSPRVEDEDKLETAPEKVLARAYDLVLNGVEVGGGSIRIHRSELQDRAFKALG
                     ISEEGRREKFGFLLDAFKYGPPPHGGIAFGLDRLSMLMAGADSLRDVIAFPKTQKGSD
                     LMTDCPTGVSKKQLDELFIQLKPDLPTKG"
     gene            complement(6470..7444)
                     /locus_tag="VMZ53_28375"
     CDS             complement(6470..7444)
                     /locus_tag="VMZ53_28375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32463.1"
                     /translation="MTFPRRLLPLLHELGTRLLESRKPRTELAHLARDLMRGFAALCT
                     RYGFDALLPLTEDPAREAALTTQLATADIDGGGPRNARPLKLAECLIAALGIELVDEP
                     DKTITLDGTVRKDVVSTISKVVEEELAVPKLRDAIIAKARSKIEEQYFIPFQKISAEL
                     DERGMRITKQLKLPLDAVRAVQRSLDDARNGLLEAMTRTAIDRAKAVIARANPEAAER
                     IDQPVTHVLTPRDATVARVIDPRLPKLPSSVTDAIVDGLGELASISWRADEKPVRPYS
                     PKDTYKVGELIEHPKFGRGTVTAVSNSMPRVDIELADGKVITLVHVRK"
     gene            complement(7441..8106)
                     /locus_tag="VMZ53_28380"
     CDS             complement(7441..8106)
                     /locus_tag="VMZ53_28380"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HUS32464.1"
                     /translation="MSTARTLGQFVADEKIVEWLSAQIADNHKQSARGATAVAKQHRL
                     PMVCSTCDVTKACCSSYVLVRLYEGLVVAAELARTGRDTPELRDQLRARAELMESTPV
                     AEWFTPCLFLDATERCTVYAARPTTCAQLYVYSAPQLCVARSNEIKSYTPRDEVAAAN
                     ALEEQFREKLSLRKKVGRRYFGVLPRMVLVSLEAWDRTDFRDYLRQLPWRTDEEWADM
                     VTR"
     gene            complement(8112..8279)
                     /locus_tag="VMZ53_28385"
     CDS             complement(8112..8279)
                     /locus_tag="VMZ53_28385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32465.1"
                     /translation="MAQENWWNSSFEDMMTDFGTWLSGRPMITIVHLDWKYVQRPTES
                     DGFWYMLCVYK"
     gene            complement(8297..9250)
                     /locus_tag="VMZ53_28390"
     CDS             complement(8297..9250)
                     /locus_tag="VMZ53_28390"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUS32466.1"
                     /translation="MTAAVSKLPVPSDDELLDAYSKAVIGVVERAGPAVVSLEIGGAR
                     GPGGAGSGFVVTPDGYVMTNSHVVSNARTIKVRTSTGDEVEARTVGEDPATDLALVRV
                     DAPLPYLSVDGSVAPRVGQLAVAIGNPLGFESTVSTGVVSALGRSLRGRGNRLIDGVI
                     QHTAPLNPGNSGGPLLDTSGRVIGVNTAIIARSQSIGFAIAVETAGWVLGQLLQHGRV
                     RRAWLGIGAARRPLDRRLSYHHGLGAGAVEVQSVEPGSPAARAGLLDGDLLVRFNDSA
                     IESVEALHKALRSAAAGAKIRLGLLRRGQRIDLEITPVWAA"
BASE COUNT         1604 a         3000 c         3098 g         1592 t
ORIGIN      
        1 aatcggcgac gccggcgatg aacgggatgc gcatcgtcag gccggcggct gcgtcgacct
       61 cggcgaggtc tcccgggatg ccgcgatgga tgccttcgac gtaaacgccg agtgcccatg
      121 ctcccgcgaa gaacgcagcg acgtgactgt cggcgacgcc gcccgcgctc gcggagtaga
      181 gctccgtcgc gaggcgcacc gcgacgccag ttgtcgtcac gcgctcgcct tgattcgagc
      241 cgctcagcat ctcgagtcgc gcgccgagcc acgtgcgcca gtgcttgtga ttctcgatgg
      301 cgtatgcgcc ggagacgtac gcgcctttga gcgacagcga gtggtcttcg ggtcccgtcg
      361 ccacgcggtc gatcgatccg ccgaagctct cgcgcgatgt cgtgacgaag ccgatgccga
      421 aatcgatcgg tgttggacgc cacgatagcg aggcccagtg aaagccgatg cggaattcgg
      481 tcgcggtgcc gatcgtgttg tcatccgcgc cgaccgcaga ggccgcgccg atttcggctt
      541 cgaacggcgg cacgacgatc gcgccgggct tcgcgtgtga gacgcgcgac gacgccaggc
      601 ctacaaccgc cgcaacaagc ccgaccaccg cgatacgcat cgcgcgcgat tgctgcaacg
      661 ggtgcgccgc gacgaggccg cgacaagatc gtgaaagtgc gggcgcgatc gcccggctcc
      721 accgacgcac cgaacgcacg ctgtggtgtc gacgcaacat gcctgccctg cggtacaacg
      781 cgagcgcgat gggcgtcttc caccacctct cgatcggcgt cgccgacctc gcgcgctccg
      841 ccgcgttcta cgacgctgcg cttgcgccgc tcgggctcgt ccgcatcttc gagaatccgc
      901 gcgctgtttg ctacggaccg cccggcttcg agggcgaagc gccgttcgcg ctcctcggca
      961 aaggcgccga tgcgaagccg cccggcatcg gctttcatct cgcgttcacg gcgccgagcc
     1021 acgaggctgt gcatcgcttt cacgaagcgg cggtcgcggc cggcggcgtc gacgagggac
     1081 cgcccggcac gcgcgagaac tattcgccgg actactacac ggcattcgtg cgcgacctcg
     1141 acggctatcg cctcgaagcg gtcgtctaca aggagcccgc gtgaaggtct cgcgagtcat
     1201 cgccgcgccg cgcagccgcg tgttcgccgc gtggatgagc aaggacgccg tcgcggagtg
     1261 gttgccaccc gcgggaatga gcgctcgcat cgatcacttc gatgcgcgca tcggcggcgg
     1321 ctatcgcatg acgttgacgt acgacggaag cggtcacggc aagacgacga ccgacaccga
     1381 tgttgtcaac gtgacgttcg acgagatcgt cctcgacgaa cgcatcgtgc aagcgacgac
     1441 gttcgacacg aaggatgccg cgtttgccgg cacgatgacg ctgacgtgga cgttcgcgga
     1501 cgcggagccg ggcggcggta cgcgcgtgac cgtcgaggtc gccaatgcgc cgcgcggaat
     1561 cacggatgcc gatcacgaga aggggatttc gtcgacgctc gacaacctcg cgacgtacgt
     1621 cacgcgctga cgtacgatgt gaggccgtgg ctgcgaaccg catgctgatc gtcgttgcgc
     1681 tctcgagcgc gtgcggccgc ttcagcttcg acgagcgcga gcttgatgcg aagcaagaca
     1741 tcgatgcggt cgcgcgctgc accgcgttcg gcccgtggag caaacccgag atgccgcaag
     1801 acatctcgtc ggatgccgag gagtacaacg gcacgatcga gagcagcggc acgaccatca
     1861 tttttccgtc gggacgagcg ggcggaatgg ggtcggctga tctgtatatg gcgacgcgac
     1921 cgtccacctc ggagccgttt gcgacacctg tgaacctcgc aaacgtcaac tcggtcgcgg
     1981 ccgatatggc ccccacactg tcgaacgacg agctgacgct ctacttcacg tcgaatcgag
     2041 gcggcgggcc atttcggctg tacgaagcga cgcgtgcatc gcgagcgcta cccttcggcg
     2101 tgccctctct cgtgcccgga cctttcacgg ggctcggcga agtcggtggt cccgacatct
     2161 cgcgcgatgg cctggcgctg tacttcgacg caaccgccgg cggcaccggc ggctatgacc
     2221 tctgggtagc gacccgcgcg agcgtgggcg atccatgggg aacgccgtcg gaagtttccg
     2281 gcgtcaacac gaccggaagc gagaacgagc cgacgatcga cgatgacggg ctgacgattt
     2341 actacgcgtc cgatgcattc gaaccggcgc aggatctggt cgtcgcgaca cggcccgatc
     2401 tcgcatcgcc gttttcgacg ccgacgaaag tgcccctctc actcaaccct ggcgtcggcg
     2461 aatacggtgc ggacatctcc gcagatggct tatggatgga gctgtcgatc catctcggcg
     2521 caccgatgaa cacgaacgtg tggctctctt cgcgcgtgtg tctctaggcg atcgcggagg
     2581 cgagccgcgc gccgtcggtc gtcgaccacg cgtcgatcgc gctctcgacg gtcgcgtcga
     2641 tgtcgtcgga gagctgcgcc gtcgacaccc acgggaagct ctcggcgagc acaccgtgca
     2701 gctcgctcgt ggcggcgagg atcgcgacgg cgacgtggag ctgcgtgcgc gtgagcaagt
     2761 cgatcgcttc gagcggcgtg cgcggaacga gcggtgtcat cccgcgagcg gcgaggcgat
     2821 tggcgagctc gacgcgccgc gagcgtttgt catcgacgac gagaacgggg cggcgctgcg
     2881 ccgcgcgcac ggcatgctcg atcgcggtgt cgatcgcagc gcgcacgttg tcttcgaggc
     2941 gatcaaatgt caccgcgatg cgcaggcgcg tcggcgagta gcgctcgatg cgaacggtgt
     3001 gagcgctcac ccagcctgtc tcgtcgccga gcttcagctc gagatcgaga tcgttctccg
     3061 acgacggcac gcccatcacc gtgagtagag cgccgctcgt cgagagattc tcgatcgtgc
     3121 cgtgggtcgc gccgtcttct gtgtagaaca cagctacgcc tctgaccggg acgcgcaacg
     3181 cactcctccg ctccgcgaga tcgcccatgg aagccccctt cgaatgcgga gcatacgaaa
     3241 agcgatcgca atccgatccg acttttttcg ctgcgcagcg cgtcagctgt cgtttgcgct
     3301 attggtgcaa tcggccgcga aaccgacgcg aatcgcgcgc atcaatcacg actcaatatg
     3361 taaggagcgc atcgagatgg cgacgctccc acagcttcgc ctcgtcgtag tgctcgtaca
     3421 tcgcttcgtc ggcgaggaac tccttcgagt ggaactcccg gcggccgttc tttacacgga
     3481 tgtcatgcac ctgatgaagc atctcgtgaa acacgatcca cgcgacgaag aagcgcggca
     3541 cgaatgatcg atccaacgag cggtggatcc ggatgaggcg gtcctcgacc gagtagctgc
     3601 ccatcttgat cgagttgcgg cggcgcggac gaccggtgcg ctggccccac gtgatcgcgg
     3661 cgtcgatctt gttatcgaag taacgcgcgt tgaggtcgtc gtagatctcg cgcagatcgt
     3721 ggtgctcgcc tgcggtgaag atgacttgcg tcggctggcg acgcgcacgg ccgcgaacgt
     3781 ggctcgtgtt ctgatcgatg aagtcgccga gcacgcgcga cgcttccgcg tcgttgtccg
     3841 cgatgtagcg cgcgagcgct cgcgtgatca ccgggtcggc gtccgcgaac atgtgatgga
     3901 gccgcacttc gtagcgcttc tccttcgcga gccggcggac cgagatcatc gtgtagcgat
     3961 tgtcggtgag cgtgaccgcg acgcgactct tcgcgaggtg cgcgcgaatg cggcgctcga
     4021 gagaatcctg cgcgtcgacg atctcgctcg ggatatcggg tccccgcttc ttgcggagag
     4081 catcagccag tgcagccgcg tagctcgcgc tgtcgtcgtt gatcgtgtct acgacggcac
     4141 agggatccgc gaacggtgca gctggtgtga ctggcaagag cgcttcactc aaaacgggcg
     4201 actcctctta tttaagcaga catcgaaccg atggcctacg gcgcaccgcg gacgcacctc
     4261 tggcggtgcg ggcctggccc ggctcgttcg acgttccccc ccgtgacccc gaacccgacg
     4321 aaaaaaatcg tatcgcgtcg tttctcgatg gatcaaattc ctgactgatc gagggatcgg
     4381 atctgatcgc aactactggg aaatctggat gtgggcgggg atcgcaccgg atcgttagaa
     4441 accgggtaga gtgcgcgttc ccatgagcca gttcctcgcc accaatactc ggacccatag
     4501 ctgcggccag ctccgcgccg gcgatgtggg caagcgcgtc gtcctgaccg ggtgggtcaa
     4561 gacgtaccgc gaccacggca aggcagtgtt catcgatctc cgcgaccgcg acggcctcac
     4621 gcagctcgtc ttcgacgaga gcttctccaa gcaggcaacc cagctcgcaa acgagctacg
     4681 aagcgagtgg tgcatcggcg tggtcggcga ggtccggtcg cgcggctcga acgtgaacga
     4741 aaagcttccg acgggatcga tcgaggtgtg ggtcgagacg ctcgaggtgt tctcgaaggc
     4801 cgagacgcca ccattccaga tcgaggacca agtcgacacg aacgatcagg tgcgcggcaa
     4861 gtaccgctat ctcgatctgc gccgcccgaa gctgcagaag aacctcatga tgcgctcggc
     4921 gatcacgcgc gtgacgcgtg acttcctcgg caagaatggc ttcctcgaga tcgagacgcc
     4981 cttcatggtg aagtacacgc cgggtggcgc gcgtaacttc ctcgtgccgt cgcgtctgaa
     5041 ccccgggcaa ttctacgcgc tcgcggagtc gccgcagatc ttcaagcagc tgctgatggt
     5101 cgcgggctac gagcggtact tccagatcgt gcgctgcttt cgcgacgagg atctccgcct
     5161 cgatcgccag ccggagttca cgcagatcga catcgagatg tcgttcgtca acgagcagat
     5221 cctccagtcg acgatggagg gcctcatctc gacgctgtgg cgcgaggtgc tgggcgtcga
     5281 gctccagctg ccgctgcggc gcatgccgta cgccgaggcg atggagaagt acggcgtcga
     5341 caagccggac ctgcgctgcg agctgcagct ctgcgaggtc accgacgtcg cgcgcacgtg
     5401 cggcttccgc gtgtttcaag atgtcgtcga gaagggcggc atcgtgaagt gcctccgcgt
     5461 gcccggcccg gccgacaagc tgccgcggtc tatcctcgat gggttgaacg acttcgcgaa
     5521 gtcattcaac gcgaaggccg cctgggctcg tgtccaagag ggtggccagt ggcagggcat
     5581 caagggcctc accgacgagt cgcgcgccga gattaaccgc atcgccggcg cgacgcctgg
     5641 tgacacgctg atcttcgtcg ccgacaaggc gaaggtcgcg aacgcgacgc tcggtgcgat
     5701 ccgcatccat tacatcggcg agaagctcgg cctcattcgc aaaggcgagt ggcagttcat
     5761 gtggctcacc gatccgccgc tcttcgagat cgaggacggc gaggtcgcag ccgcgcacca
     5821 cccgttcacg tcgccgcgcg tcgaggacga ggacaagctg gagaccgcgc ccgagaaagt
     5881 cctcgcacgt gcgtacgacc tcgtgctcaa cggtgtcgag gtcggcggcg gatcgattcg
     5941 tatccatcgc agcgagctgc aggaccgcgc gttcaaggcg ctcggcatct ccgaagaagg
     6001 ccgccgcgag aagttcggct tcctcctcga cgcgttcaag tacggccctc ccccgcacgg
     6061 cggcatcgcg tttggtctcg accgcctctc gatgctgatg gcgggtgcgg actcgctgcg
     6121 cgacgtcatc gcgttcccga agacgcaaaa gggcagcgat ttgatgaccg actgcccgac
     6181 cggcgtgtcg aagaagcagc tcgacgagct gttcatccag ctcaagcccg atctgccgac
     6241 gaagggctag gcgacggccc gcgaggcagg cggggaatga acagggagac atggagacac
     6301 gaagtccgga gggcggatcc tgacctgccg gatcgcggag cctgacgacc cggatcgagg
     6361 atcctgcgcg cccccgatcg aggagccctg ccctctgttc tgatctccgt gtgctccgtg
     6421 tctccgtgtt gattcgatcc gatcagccga ggtgcccagc gccgttccgc tactttcgaa
     6481 cgtggacgag cgtgatgact ttgccgtcgg cgagctcgat gtcgacgcgc ggcatcgagt
     6541 tggacacggc ggtgacggtg ccgcggccga acttcgggtg ctcgatgagc tcgccgacct
     6601 tgtacgtgtc cttcgggctg tacggccgca ccggcttctc gtctgcgcgc cacgagatgc
     6661 ttgctagctc gccgagcccg tcgacgatcg cgtcggtgac cgacgacggc agcttcggaa
     6721 ggcgcgggtc gatcacgcgg gcaactgtgg cgtcgcgtgg cgtgagcacg tgcgtgacgg
     6781 gctgatcgat tcgctccgca gcctcggggt tcgcgcgcgc gatcacagct tttgcgcgat
     6841 cgatcgcggt gcgcgtcatg gcttcgagca ggccatttcg cgcatcgtcg agggagcgct
     6901 gcacggcgcg gactgcgtcg agcggcagct tgagctgctt cgtgatgcgc atgccgcgct
     6961 cgtcgagctc ggcggaaatc ttctggaacg ggatgaagta ctgctcttcg atcttcgagc
     7021 gtgctttcgc gatgatcgcg tcgcgcagct tgggaacggc gagctcctcc tcgaccacct
     7081 tcgagatcgt cgagacgacg tctttgcgca cggtgccgtc gagcgtgatc gtcttgtccg
     7141 gctcgtcgac gagctcgata ccgagcgcgg cgatcaggca ctcggcgagc ttcagtgggc
     7201 gggcgttgcg aggccctccc ccatcgatgt cggcggtggc gagctgagtg gtgagcgcgg
     7261 cttcgcgcgc gggatcctcg gtgagaggaa gcagagcgtc gaagccgtag cgggtacaga
     7321 gagctgcgaa accacgcatg agatcgcgag cgagatgcgc gagctccgtt cttggtttgc
     7381 ggctctcgag cagccgcgtg cccagctcgt gcagcaaggg gagcaagcga cgggggaacg
     7441 tcatcgcgtg accatatcag cccattcctc gtcggtgcgc catggcagct gccgaagata
     7501 gtcgcggaag tcggtccgat cccaggcctc gagcgagacc aggaccatgc gcgggagcac
     7561 gccgaagtag cggcgtccga ctttcttgcg caaggacagc ttctcgcgaa attgctcctc
     7621 gagggcattc gcggcggcca cttcgtcacg tggtgtgtac gacttgatct cgttcgagcg
     7681 cgcgacgcac agctggggcg ccgagtagac gtagagctgc gcgcaggtgg tgggccgcgc
     7741 cgcgtagacc gtgcagcgct cggtggcgtc gaggaacaag cacggcgtga accactcggc
     7801 gacgggcgtc gactccatga gctcggcgcg ggcgcgcagc tggtcgcgaa gctccggcgt
     7861 gtcacgtccc gtgcgcgcga gctcggcggc gacgacgagg ccttcgtaga gacgaacgag
     7921 cacgtacgac gagcagcacg ctttcgtcac gtcgcacgtc gagcagacca tcggcagacg
     7981 gtgctgcttt gcgacggcgg tggcgccgcg cgccgattgc ttgtggttgt ccgcgatctg
     8041 ggcgctgagc cactcgacga tcttctcgtc ggctacgaac tgcccgagcg ttcgggctgt
     8101 gctcatcgac gtcacttgta gacgcagagc atgtaccaga agccgtcgga ctccgtcggt
     8161 cgctgcacgt acttccagtc gaggtggacg atcgtgatca tcgggcggcc cgagagccag
     8221 gtcccgaagt ccgtcatcat gtcctcgaag gacgagttcc accaattctc ttgcgccatg
     8281 cgcaagacag tagatctcag gcggcccaca cgggcgtgat ctcgaggtcg atccgctggc
     8341 cgcgtcgcag aaggccgagc cggatcttcg ctcccgcggc tgccgaccga agcgccttgt
     8401 gcagcgcctc gacgctctcg atcgcgctgt cgttgaagcg cacgagcaag tcaccgtcga
     8461 gcaggcccgc tcgcgcagcc ggtgatccgg gctcgaccga ttggacctcg accgccccgg
     8521 caccgagacc gtggtgatac gagagcctgc gatcgagcgg cctgcgcgct gcaccgatac
     8581 cgagccacgc gcgccgcacg cgaccgtgct gcaacagctg gccgagcacc cagcccgcgg
     8641 tctcgacggc gatcgcaaag ccgatgctct gcgaacgcgc gatgatcgcg gtgttcacgc
     8701 cgatgactct gccgctcgtg tcgagcagcg ggccgccgga gttgccggga ttgagcggtg
     8761 cggtgtgctg gatgacgccg tcgatgaggc gattgccgcg acctcgcaag ctgcggccga
     8821 gcgccgacac gacgcccgtc gacaccgtcg actcgaagcc aagcggattg ccgatcgcga
     8881 ccgcgagctg tccgacgcga ggtgcaacgg agccgtcgac cgagagatac ggcagcggcg
     8941 cgtcaacccg aacgagtgcg agatcggtcg ccggatcttc gccgacggtg cgagcctcga
     9001 cctcatctcc cgtcgacgtc cgaactttga tcgtccgcgc attggagacg acgtggctgt
     9061 ttgtcatgac gtaaccgtcg ggcgtcacga cgaacccgga gcccgcgccg ccagggccac
     9121 gcgctccacc gatctcgagg ctcacgactg ccggaccggc tctttcgacg acgccgatga
     9181 ccgccttcga gtacgcgtcg agcaattcat cgtcgctggg cacaggtagc ttcgagaccg
     9241 cggcggtcac gccttcgccc cgagcgtgac gtcgacatcc tggcgattac cacc
//
LOCUS       DATLBZ010000572        14458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_4465872, whole genome shotgun sequence.
ACCESSION   DATLBZ010000572 DATLBZ010000000
VERSION     DATLBZ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14458
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4465872"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VMZ53_28395"
     CDS             <1..183
                     /locus_tag="VMZ53_28395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32467.1"
                     /translation="EDADKSVSLGGENALWFQLESALSPVLTPWARDRALGEWAATWG
                     SHRSYYSGTRPPGQGL"
     gene            180..2315
                     /locus_tag="VMZ53_28400"
     CDS             180..2315
                     /locus_tag="VMZ53_28400"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HUS32468.1"
                     /translation="MTASDADLEMSFYDKFLLSKGLEPYPVQEQAISLIYQGKSVLVT
                     VPTGTGKTVMAKAALHRAMGRGEKAIYTTPLRALTEEKFRELSEDFGEENVGFATGDY
                     KVNREAPIQVEVAEILWNRIVAEKHVSPADIVVMDEGHYFNDPERGYVWEQSIIGLDP
                     RTQLVILSATVGHPERFCQWVEVTRRLPLMLVESRERKVPLVHEFREEMLIDTVRDLA
                     HTGDVPAIIFIFGREQCFEVARLLKSCRRFTTDEEKAKIEKLCDEALLPAGVAKELRP
                     LLGHGIGIHHAGILPRYKQLVEQLALERLIKFVVSTETIAAGINLPARTVVFPSLRKF
                     IKQQPRLLTSAEYHQMAGRAGRPQFDDRGLAITLAPEQVVSDLKKELKDAAKRPAYDE
                     AKVKRSVYGRAKAEAVRKQDIIWTPDEHAALMKGEPAELRSKTKITAEQVLAIGLPDL
                     TEAKLPATDAEVRMAAAEASLPPSMRLDIVTVIENLLLTDRERRELLKTLAQLVDNLK
                     AIGVIDEHGKQVSGDMIRQLQGMDGLFIFYVLFNHQLEYTELRELVEYLIDHDIVQRV
                     LDRKGEDAKREWTRTWLREQRFEGNQVTWEDAEAAYEKEHPRPLTKIELIHQEFASKV
                     PHPELHGGKRAKNTWAQLEDSGMSFLEFVEKHGLEHEEGNLFSYLVRVMNFSNKLKEA
                     SQLTEFEDMAERVQKLLAQIDLRIVERYL"
     gene            2312..3655
                     /locus_tag="VMZ53_28405"
     CDS             2312..3655
                     /locus_tag="VMZ53_28405"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32469.1"
                     /translation="MKYWLVVLALVASCKNKDIPYKPPPQDAPAMAAADAAEAVDAAT
                     AAAKLGATKVAVGDNFTCAVLSDKTLRCWGRNNEGQLGTGTTTDSPSPVAVRLVGVVD
                     VVAGTAHACALLDDRSVTCWGRINYGHAENLLVPTGVAGVAKVKRLFAVRRAACATLD
                     DDSLVCWGDVDLQGHLRLAGSTVEHRVPTPAEGLSKVVMLTVNGALHEDGTVSFWGAD
                     GVPQKTALTGVTEIAASGEEVCGLRGDGTVACVGPITVCAANAPKPPAAKKPPVKKPA
                     KKAPAKKTAKKPAAKKPAAKKPPPEPKPEPAPAPSRELALEVLALPPAKHLAFDVGLC
                     VVTKSGALQCLSARDGCKQDSPWPGLATVDRVAGYCALMRDAAVKCWDVDRKSRLVSA
                     IADATNVRDLVVSTSHACALRSDQTIVCWGSNKFGALGRGEIDDVTHREARPVRF"
     gene            3671..4507
                     /locus_tag="VMZ53_28410"
     CDS             3671..4507
                     /locus_tag="VMZ53_28410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS32470.1"
                     /translation="MARLPEPPDVPTSVYDADDDDHAFDQEEDTPIVMAAGTRDNLAD
                     QARAAAASRPTGRPPRAIDDDVASLAHDLKNPLTIIMLETSQIEQRLGLRGSPAVHRG
                     LERISQNAAYIDRLVSDLLDLTSHEAGKLELRLERVDLSRLLYDALGRAVSTVDRARV
                     TLDVRDVLYAEADEIRIERVISNLVCNALKYCPDHVVVRLDARGKFACVSVIDSGPGL
                     TAEESRLVFERYRRGTTQQQGYGLGLHTARRIVEAHRGRIGIASQPGKGSRFFFELPI
                     AR"
     gene            complement(4504..4968)
                     /locus_tag="VMZ53_28415"
     CDS             complement(4504..4968)
                     /locus_tag="VMZ53_28415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32471.1"
                     /translation="MTEDLLARFGIYGGSIAVAFIAGMFPLVSIELFLITLSAALHLS
                     LVQILTCCGIAAVSHQIAKTITYYAGVGALEHGKIGKKIENARPRIEKWNKAPKLVLT
                     LGAVFGIPPLYLLGFIAEPLMRIRFLTFTLIVFFGRLVRFIFLAAIPLVFDI"
     gene            complement(4965..6599)
                     /gene="glpD"
                     /locus_tag="VMZ53_28420"
     CDS             complement(4965..6599)
                     /gene="glpD"
                     /locus_tag="VMZ53_28420"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HUS32472.1"
                     /translation="MSVTYDLVVIGGGITGAGIARDAALRGMKVALFEKSDYASGTSS
                     KSSKMIHGGLRYLEHGEIGLVFESVSERRLQQNVAPHLVRPLPFLIPIYKGVRPGFEV
                     MNLGLWIYDSLALFRAPKMHKAFRGATAALALEPQLRPEGLRGALEYYDCATDDARLV
                     LENALDAQALGADCHTYTEVQRFERAANGRITGVVVHDRLHGKTWTVGARAVVLAAGA
                     WTDEMIRRFEIPMDRPLLRRTKGVHVVLPRERLPLARAITLISPVDGRVMFALPWRDR
                     SVLGTTDTDFAGTADEVAADAADVAYLCASGNGYFPGANLTPDDVIATWAGLRPLIAA
                     PPNVDESEISREHEVFTKSDGLVIIAGGKLTTYRRMARETVNKTLELLRDIGETVETK
                     HVNTKTRPLPGAEGIEPTDLEGVAKIGRRLMDEYKLDVDTATHLCGVYGSRAIVLGQM
                     IATDRQLGERLDHELPYVWAEIEFAAKHDLARTVEDVLARRVPLLLTSRDQGLSVCEK
                     VAARLAQVHGWDATAVAQMIEEYRAEVALSRRWRQA"
     gene            complement(6596..7657)
                     /locus_tag="VMZ53_28425"
     CDS             complement(6596..7657)
                     /locus_tag="VMZ53_28425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32473.1"
                     /translation="MLRWLAVIALLVACKDKEQPKDVGKPKIGVNKGRITYVSPRCAQ
                     AVTDAMSAPPLSRPELLIHGCHVCGLEDLDWKPILEWNLNATRPEQLQAALETCDAFC
                     TGDSKLKFMAGADNAKGTSNDTPWRKLADTCGDRIDLSSDHRFMSAAYFALDRIARAV
                     GGAPGSHGTADKLATITLPLPALTVAGTGVVLPSAGPGPGVIASDPQSAAAADVAITI
                     LGDQIYVGKLPVAKLTAKGVEVDFGPVPYPGKETTAAELPAALKALGCDKTTWVAVLA
                     PTGMPAGKLVPIVVAAAPAVSLHLGVAAAKSPPGWQLPAMLASELSIADNGVQVTADM
                     PVQKLVALLVAKGSGTIKP"
     gene            complement(7657..9027)
                     /locus_tag="VMZ53_28430"
     CDS             complement(7657..9027)
                     /locus_tag="VMZ53_28430"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS32474.1"
                     /translation="MTSVHTQLLTDDASGLLLERSFRLRVVAGPDQGKEHMLDEGTTM
                     VGTHADNDLVLSDATVSRYHLEIRVRRDGIEVRDLDTTNGTKHGGARIGSVVLSGAAR
                     LRLGKHTEMDVEPVDTTVDIGEWPSDRFGDVLGTTQPMKRLFSLLSKAAPTEATILLQ
                     GETGTGKEAIAEAVHRHSRRNKGPFVVVDCGSIPHELIASELFGHAKGSFTGAGADKQ
                     GLIEAANHGTLFLDEIGELALDLQPQLLRVLDRRQVRRVGETQSVDVDIRVVAATHRD
                     LRAMVKAGQFREDLYYRLAVVATYVPPLRDRKADIPALATWFAEKMGRGSFAQSPMLI
                     DQLQRHDWPGNVRELRNVVERALSLGDSALADLGDGGPSRGSQPTVAETPEPATRMGS
                     NPDVLEMPFKEAKAQLVEAFERDYLTALLARHHGNISRAAAEAGIDRNYIHRLVKKYG
                     LEVDRT"
     gene            complement(9032..10309)
                     /locus_tag="VMZ53_28435"
     CDS             complement(9032..10309)
                     /locus_tag="VMZ53_28435"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HUS32475.1"
                     /translation="MKLALALVCAFATPAFAGFTMTFSGDPHPGMHRETWTDNAIPAR
                     IRLVQIDLTSSEIAAYATQESDRGITTTEFATRKNAQLAINGDAFAVNGYVPRGLAMG
                     ASNPWSQTADNTQTATFLFRREGERTVADIIPPEAVITPMDLPEGTEGVISGRPLLVR
                     QSAAVTQFDCNDSTSMPCQPAPRTAVAISPDGRTLWIAVVDGWQSGSIGVTCPQLAEF
                     LVARGAGMALALDGGSSSTLYLDGDVISNPSDGVERTVANHLAFKYGQLPKGQMYGLV
                     CKHNVIGCANDPPRKISGAQVFLDDTRMQTTDSTGVFDFLGVTPRLACVTVKKTGYQT
                     VKQCRQVVAGEITYNSVALWEGMDPPDAGVRKDAAVYPDATTAGDAQTSDGGNGYMDP
                     GGGCCESGGGDRGTVPLLVLVAFFLRRRRGTND"
     gene            complement(10328..12475)
                     /locus_tag="VMZ53_28440"
     CDS             complement(10328..12475)
                     /locus_tag="VMZ53_28440"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS32476.1"
                     /translation="MLARRIIALSPDKAFGKQLATALKAAGGAVDLHASMDSLGKGEL
                     QAALVVLHLDGELQTAAAEILPRLIGDTRVICVLPRTNLAAVVDIMQSSDRVSGLLVA
                     EDFNSQQLSAMATRVLVGDIFGLEKLIKWGTLVHSQLVGDYQEKSLCISQISEFAELM
                     GVRRKYREAVEQCVDEMLMNALYDAPVDEQGKQIFSEIPTKTRISLRVEQKVVVQYAC
                     DGHQFVVSVRDAFGTLERSTVLKYLYKCLHAPADQQIDRKAGGAGLGLYLMANSASTV
                     MFNVLPGVATEAVCVFDLETPKIQLEEIGFFTEKIDAGGRLAVGASRRLPSAGHPVER
                     RRPPDGPAPSRGLIGALAAAIVLVLALVGLAAWQRMGGAGKQMTNVTFTTVPKGATIE
                     IEGRNEGTATDGTLVRSLEVGRAYPVVAKLEGYEPKTAVVQPRAGDTAVSFELAARQA
                     TVLLDTLPSGATVEIDGKPIGTTPVTVTTLPPGSTQQVVFKKAGYRDQTTSVNVPSPG
                     KELNMVLPMQVSEDLARIKIESDPPGAQVVQNGQVLAGVTTPAEVLVEAGKPQRFTLT
                     MPDHVPAVIDAFTPGRGATGITKSAKLVAGAKLTVEANLDGKITVTPAPHCKDMTPPV
                     ECVLGPGSYAVDFVTQGGRATHSIAVKGKPMTDKFELGYVDAGEGKMLQVAGQKVKRA
                     VLEVGPRSVTVLDEAGSHQVQVRVKAGTPVVAN"
     gene            complement(12550..13884)
                     /locus_tag="VMZ53_28445"
     CDS             complement(12550..13884)
                     /locus_tag="VMZ53_28445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32477.1"
                     /translation="MLRRSVFSVGLLVLASVDAYANPADIVPGGQDPEDPNSDHAQKS
                     VDIYGQVDYAYELDSSSLIRERLGPGSDPNAAVPTVHDLKFHQFKHTITPSVHVGIFR
                     DTFFYAALPIVITQVRELELDDGVDRAGSPTVTDGLLPMQGFDARDPTTPAAGNLMFR
                     GPSRKGLDQVHLGLGIAPMNQAHDPTKPIWKLGAEIRLAVGKIMKFDPMAPNANTGVS
                     QGVQELKVWTSFARKLGWAEPWVDLWWMVPLAAKSDALFQDPGFGASNIQKSQQAGLG
                     FGLELYALDQGRDQTRISLDFGTRATVHFEGREYTEMWEAFAYAGDSRGTGPLILDSD
                     PTRADVQPLSYPGISNVENYLETHGLFAVRAQLGPHVRFSVSGDLTWKTDHLITFADA
                     GKDSPDDDNDLVNPGTDEVNPLHVPGLDLVGHRYRSYEGFDVAFGVTGQVLF"
     gene            complement(13909..>14458)
                     /locus_tag="VMZ53_28450"
     CDS             complement(13909..>14458)
                     /locus_tag="VMZ53_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS32478.1"
                     /translation="EIQKGNFREDLFYRLNVVPLQIPPLRKRTGDIPLLVGHIIKKFN
                     ERLKKSITGISDEALAALETHSWPGNIRELENVLERTILFTKGDRIERQDLQLRSAGQ
                     EPASQSQPVPMLGDDVDDAELSGSLKEVVRAETARVERELIVKALDETGGNVTQAARL
                     LKISRKSLQMKMKEFGLRDRES"
BASE COUNT         2436 a         4666 c         4897 g         2459 t
ORIGIN      
        1 gaggacgcgg acaagtccgt cagcctcggc ggcgagaacg cgctgtggtt tcagctcgag
       61 agtgcgctgt cgccggtgct cacgccgtgg gcgagagacc gcgcgctcgg ggagtgggcc
      121 gcgacgtggg ggagccatcg cagttactac agcggcacgc gcccgccggg ccagggcctg
      181 tgaccgcgag cgacgccgac ctcgagatgt cgttctacga caagttcctc ctctcgaagg
      241 gactcgagcc gtatcccgtc caagagcagg cgatcagcct catctatcaa ggcaagagcg
      301 tgctcgtcac cgtgccgacg ggcaccggca agacggtgat ggcgaaagcc gcgctgcacc
      361 gcgcgatggg ccgcggcgag aaggcgatct acacgacgcc gctccgcgcg ctcaccgagg
      421 agaagtttcg cgagctgagt gaagacttcg gcgaggagaa cgtcgggttt gcgacgggcg
      481 actacaaggt gaatcgggaa gcgccgatcc aggtcgaggt cgccgagatt ctatggaatc
      541 ggatcgtcgc cgagaagcac gtatcgcccg ccgacatcgt cgtcatggac gagggccact
      601 acttcaacga tcccgagcgc ggctacgtct gggagcaatc gatcatcggt ctcgacccgc
      661 gcacgcagct ggtaatcctc agcgcgacgg tcggccatcc cgaacgcttc tgtcagtggg
      721 tcgaggtgac gcgccgattg ccgctgatgc tcgtcgagtc gcgcgaacgc aaggtgccgc
      781 tcgtgcacga gttccgcgag gagatgctga tcgacacggt gcgcgacctg gcgcacacgg
      841 gcgacgtccc ggcgatcatc ttcattttcg gtcgcgagca atgcttcgag gtcgcgcgcc
      901 tgctcaagtc atgccgtcgc ttcacgaccg acgaagagaa ggcgaagatc gagaagctct
      961 gcgacgaggc gctgctgcct gccggtgtcg ccaaagagct gcggccattg ctcggtcacg
     1021 gcattggcat tcatcacgcc ggcatccttc cgcgctacaa gcagctcgtc gagcagctcg
     1081 cgctcgagcg gctcatcaag ttcgtcgtgt cgaccgagac gatcgctgct ggcatcaact
     1141 tgccggcgcg cacggtcgtg tttccgtcac tgcgcaagtt catcaagcag cagccgcgct
     1201 tgttgacgtc ggccgagtat caccagatgg cgggacgggc agggcgcccg cagttcgacg
     1261 atcgcggctt ggcgatcacg ctcgcgcccg agcaagtggt gagcgacctc aagaaggagc
     1321 tcaaggacgc cgcgaagcgg cccgcatacg acgaggcgaa ggtgaagcgc agcgtctacg
     1381 ggcgcgcgaa ggcggaggct gttcgcaagc aagacatcat ctggacgccc gacgagcacg
     1441 ccgcgctcat gaagggcgag cccgcggagc tgcgcagcaa gacgaagatc acggcagaac
     1501 aggtgctcgc gattggcttg cccgatctca ccgaggcgaa gctccctgcg acggacgcgg
     1561 aagtgcgcat ggccgcggcc gaagcgtcgc tgccgccctc gatgcgcctc gacatcgtca
     1621 ccgtgatcga gaatctgctg ctcacggacc gcgagcggcg cgagctattg aagacgctcg
     1681 ctcagctcgt cgacaacctc aaggcgatcg gcgtcatcga cgagcatggc aagcaggtgt
     1741 cgggagacat gatccgccag ctgcagggca tggatggcct gttcatcttc tacgtgctgt
     1801 tcaaccacca gctcgagtac acggagctgc gcgagctcgt cgagtacctg atcgatcacg
     1861 acatcgtgca gcgcgtgctc gatcgcaagg gcgaagacgc gaagcgcgag tggacgcgca
     1921 cgtggctacg cgagcagcga ttcgagggca accaggtcac ctgggaggat gcggaggctg
     1981 cgtacgagaa agagcatccg cggccgctca cgaagatcga gctgattcac caggagttcg
     2041 cgtcgaaggt tccgcacccc gagctgcacg gcggcaagcg cgcgaagaac acgtgggcgc
     2101 agctcgaaga cagcgggatg tcgttcctcg agttcgtcga gaaacacggt ctcgagcacg
     2161 aagaaggcaa cttgttctcg tatctcgtgc gcgtgatgaa cttctcgaac aagctgaaag
     2221 aggcgtcgca gctcaccgag ttcgaggaca tggcggagcg cgttcagaag ctgctcgcgc
     2281 agatcgatct gcgcatcgtg gagcgctacc tttgaagtac tggctcgtgg tgctcgcgct
     2341 cgtagcgagc tgcaagaaca aagacattcc gtacaagccg ccgccacagg acgcgcccgc
     2401 gatggcagcg gccgatgcag ccgaggcggt cgacgccgct acggcagcgg cgaagctcgg
     2461 ggcgacaaag gtcgcggtcg gcgacaattt cacctgcgcg gtgctctccg acaagacgtt
     2521 gcggtgttgg ggtcgcaaca acgagggcca gctcggcacc gggacgacga cagactcgcc
     2581 atcgcccgtg gccgtgaggc tcgtcggcgt tgtcgatgtc gtcgcgggta cggcgcacgc
     2641 gtgcgcgctg ctggacgatc gctcggtcac gtgctggggc cgcatcaact acggccacgc
     2701 cgagaatctg ctcgtgccga cgggcgtggc cggcgtcgcg aaggtgaagc ggctcttcgc
     2761 ggtgcgacgg gctgcatgcg cgacgctcga cgacgactcg ctcgtgtgtt ggggcgatgt
     2821 cgatctgcaa gggcatctgc gcctggcggg ctcgacggtc gagcatcgcg tgccgacgcc
     2881 ggccgagggc ctgagcaagg tcgtgatgct caccgtcaac ggagcgctgc acgaggacgg
     2941 cacggtgtcg ttctggggcg ccgatggcgt gccgcagaag accgcgctca ccggggtcac
     3001 cgagatcgcg gcgagcggtg aagaagtctg cggcctccgt ggcgatggga ccgtcgcgtg
     3061 cgtcggaccg atcaccgtgt gcgccgccaa cgcgccgaag ccgccagcgg cgaagaagcc
     3121 gccggtgaag aagcccgcga agaaagcgcc cgcaaagaag acggccaaga agcccgcggc
     3181 caagaagccc gcagccaaga agccgccgcc cgagccgaag cccgagccgg cgccggcgcc
     3241 gtccagagag ctcgcgctcg aggtcctcgc gctgccgcct gcgaagcatc tcgcgttcga
     3301 cgtcggcctc tgcgtcgtga cgaagtcagg cgccctgcag tgtctgtccg cgcgcgacgg
     3361 ttgcaagcag gactcgccgt ggccaggtct cgcaactgtc gaccgcgttg cgggctattg
     3421 cgcgctcatg cgcgacgctg ccgtgaagtg ctgggacgtc gatcgtaaga gccgcctcgt
     3481 ctccgcgatc gccgatgcga cgaacgtgcg agatctagtg gtgagcacct cacatgcatg
     3541 tgcgcttcgc tccgaccaaa caattgtttg ttggggctca aacaagtttg gggcgttagg
     3601 gaggggtgag atcgacgacg tcacgcatcg agaagctcgc ccggtccgat tttagagtga
     3661 caataggtcg atggcgcgac ttccggaacc gccagacgtg cccacgtcgg tttacgacgc
     3721 ggacgacgac gaccacgcgt tcgatcagga ggaagacact ccgatcgtca tggccgccgg
     3781 cacgcgcgac aacctcgcgg accaagcgcg tgcggcagcc gcttcccgcc ccacgggacg
     3841 tccgccacgc gccatcgatg acgacgtcgc atcgctcgca cacgacctca agaatccgct
     3901 cacgatcatc atgctcgaga cgagtcagat cgagcagcgg ctcggcctac gcggctcacc
     3961 cgcggttcat cgcggcctcg agcggatctc gcaaaacgcc gcgtacatcg accgcctcgt
     4021 ctccgacctc ctcgatctca catcgcacga agcgggcaag ctcgagctgc ggctcgaacg
     4081 ggtcgatctc tcacgtctgc tctacgatgc gctcggtcgc gcggtatcga cggtcgatcg
     4141 tgcgcgcgtg acgctcgacg tccgcgacgt cctctacgcc gaggccgacg agattcgcat
     4201 cgaacgcgtg atctcgaacc tcgtctgcaa tgcgctcaag tactgccccg atcacgtcgt
     4261 cgtgcgcctc gacgcgcgcg gcaagtttgc ctgcgtgtcg gtgatcgact ccggtcccgg
     4321 cctcaccgcc gaagagtcgc gcctcgtgtt cgagcgctac cgccgcggca cgacccagca
     4381 gcagggctac ggcctcggcc tgcacaccgc tcgccggatc gtcgaggcgc atcgcggccg
     4441 catcggcatc gcgtcgcagc cgggcaaagg ctcgcgcttc ttcttcgagc tgccgatcgc
     4501 ccgctagata tcgaagacga gggggatggc ggcgaggaag atgaagcgga cgaggcggcc
     4561 gaagaagacg atcagcgtga acgtcaggaa gcggatccgc atcagcggct cggcgatgaa
     4621 gccgagcaga tagagcggcg ggatgccgaa caccgcgccg agcgtgagca cgagcttggg
     4681 cgccttgttc cacttctcga tgcgcggccg tgcgttctcg atcttcttgc cgatcttgcc
     4741 gtgctcgagc gcgccgacgc ccgcgtagta cgtgatcgtc ttcgcgatct ggtggctcac
     4801 cgcggcgatt ccgcagcacg tgaggatttg gacgagcgag aggtgaaggg cagcgcttag
     4861 cgtgatgaga aacagctcga tcgatacgag cggaaacata ccggcgataa acgcgactgc
     4921 gatcgagccg ccgtagatgc cgaagcgcgc gaggaggtct tcggtcacgc ttggcgccag
     4981 cgccggctca acgcgacttc ggcgcggtac tcctcgatca tctgcgcaac tgccgtcgcg
     5041 tcccagccgt gcacctgcgc gaggcgcgcg gcgaccttct cgcacacgct gaggccttga
     5101 tcgcgcgagg tgagtagcag cgggacgcgg cgcgcgagga cgtcttcgac ggtgcgcgcg
     5161 aggtcgtgct tcgccgcgaa ctcgatctcg gcccacacgt acggcagctc gtggtcgagg
     5221 cgctcgccga gctggcggtc tgtggcgatc atctggccga gcacgatcgc gcgcgagccg
     5281 tacacgccgc acaggtgcgt cgcggtgtcg acgtcgagct tgtactcgtc catcaggcga
     5341 cggccgatct tggccacgcc ttcgaggtcc gtcggctcga tgccttcggc gccgggcagc
     5401 gggcgcgtct tcgtgttgac gtgcttcgtc tcgacggtct caccgatgtc gcggaggagc
     5461 tcgagcgtct tgttcacggt ctcgcgcgcc atgcgccgat atgtcgtcag cttgccgccc
     5521 gcgatgatca cgaggccgtc gctcttcgtg aagacttcgt gctcgcgcga aatctcggac
     5581 tcgtcgacgt tcggcggcgc cgcgatcaga gggcgtaggc cagcccacgt cgcgatcacg
     5641 tcgtcgggcg tgaggttcgc gccggggaag taaccgttgc cactcgcaca gaggtatgcg
     5701 acgtcggccg cgtcggcggc aacctcgtca gcggtgccgg cgaaatcggt gtcggtcgtg
     5761 ccgagcacgc tgcgatcgcg ccagggcagg gcgaacatca cgcgcccatc gacgggcgag
     5821 atcagcgtga tcgcgcgagc gagtggcagt cgttcgcgcg gcagcacgac gtgcacgccc
     5881 ttcgtgcgac gcagcagcgg ccggtccatc gggatctcga agcgccggat catctcgtcg
     5941 gtccaggcgc ctgccgcgag cacgacggcg cgcgcaccca ccgtccacgt cttgccgtgc
     6001 aagcgatcgt gcacgacgac gcctgtaatc ctgccgttgg ccgcgcgctc gaagcgttgg
     6061 acctcggtgt acgtgtggca gtcggcgccg agtgcctgcg cgtcgagcgc gttctcgagg
     6121 acaaggcgcg catcatcggt cgcgcagtcg tagtactcga gcgcgccgcg cagtccctct
     6181 ggccgaagct gaggctcgag cgcgagtgcc gcggtcgctc cgcgaaatgc cttgtgcatc
     6241 ttcggcgcgc ggaacagcgc gagcgagtcg tagatccaaa ggccgaggtt catgacctcg
     6301 aagccgggcc tgacgccctt gtagatcggg atcaagaacg gcagtggccg caccaggtga
     6361 ggcgcgacgt tctgctggag ccgccgctcg ctgacgctct cgaagacgag gccgatttcg
     6421 ccgtgctcca gatagcgaag cccgccgtgg atcatcttcg acgacttcga cgacgtaccc
     6481 gacgcgtagt ccgacttctc gaacagcgcg accttcatgc cgcggagcgc tgcatcgcgc
     6541 gcgatacccg cgccggtgat gccgccgccg atcacgacga ggtcgtacgt cacactcacg
     6601 gcttgattgt accggacccc ttggcgacga gcagggcgac aagcttctga acgggcatgt
     6661 cggcggtcac ctgaacgccg ttatcggcaa tggagagctc agacgcgagc atggcaggca
     6721 gctgccagcc cggcggcgat ttcgccgcgg cgacgccgag atggagcgag accgccggtg
     6781 ccgccgcgac gacgatcggc acgagctttc ccgcaggcat ccctgtcggt gcgagcaccg
     6841 cgacccatgt cgtcttgtcg caaccgaggg ccttcagcgc cgcgggcaac tctgctgcgg
     6901 tcgtctcttt gccaggatat ggaacgggtc cgaagtcgac ctcgacgccc ttcgcggtca
     6961 acttcgcgac cggcagcttg ccgacataga tttggtcacc gaggatcgtg atcgcgacgt
     7021 ccgcggctgc ggcggactgc gggtccgaag cgatcacgcc tggccctggt ccggcgctcg
     7081 gcaggacaac gccggtgccg gcgacagtaa gtgccggcaa cgggagcgtg atcgtcgcca
     7141 gcttgtcggc agttccgtgg ctgccgggcg cgcccccgac ggcacgcgcg atgcgatcga
     7201 gcgcgaagta cgcggcgctc atgaagcggt gatcgctcga cagatcgatt ctgtcaccgc
     7261 acgtatcggc gagcttgcgc cacggcgtgt cgttcgacgt gccctttgcg ttgtccgcgc
     7321 cggccatgaa cttcagcttg ctatcgccgg tgcagaatgc gtcgcacgtc tcgagagctg
     7381 cttgcagctg ttccggacgc gtcgcgttga gattccactc gaggatcggc ttccagtcga
     7441 gatcctccag gccgcacaca tggcagccat ggatgagcag ctcgggccgt gacaggggag
     7501 gcgcggacat cgcgtcggtg acagcctggg cgcatcgcgg cgacacgtag gttatgcgtc
     7561 ccttgttcac gccgatcttc ggcttgccga cgtccttcgg ctgctccttg tccttgcacg
     7621 cgacaagcaa cgcgatgacc gcgagccacc gcagcattac gtgcgatcga cttcgaggcc
     7681 gtacttcttg acgaggcgat ggatgtaatt gcgatcgatg cccgcttcgg ccgcggcgcg
     7741 agagatgttg ccgtggtgcc gcgcgagcag cgccgtcagg taatcgcgct cgaaggcctc
     7801 gacgagctgc gccttcgctt ccttgaacgg catctcgagc acgtcgggat tgctacccat
     7861 gcgcgtcgcg ggctcgggcg tctccgcgac cgtcggctgg ctgccgcgcg aggggccgcc
     7921 atcgccaaga tctgcgagcg cggaatcacc gagggagagc gcgcgctcga cgacgttgcg
     7981 aagctcgcgg acgttgcccg gccagtcatg ccgctgcagc tgatcgatca gcattggcga
     8041 ctgcgcgaag ctgccgcgtc ccatcttctc ggcgaaccac gtcgcgagcg cggggatgtc
     8101 ggccttgcga tcgcgcagcg gcggaacgta cgtcgccacg acagcgaggc ggtaatagag
     8161 atcctcgcgg aactggccgg ctttgaccat cgcgcgcaga tcgcggtgcg tggcggcgac
     8221 gacgcggatg tcgacgtcga cggattgcgt ctcaccgacg cggcgcactt gccggcgatc
     8281 gaggacgcgc aggagctgcg gctgcagatc gagtgcgagc tcgccgatct cgtcgaggaa
     8341 cagcgtgccg tgattggcgg cctcgatcag gccctgcttg tcggcgccgg cgccggtgaa
     8401 gctgcccttc gcgtgaccga acagctccga tgcgatcagc tcgtgcggga tcgatccgca
     8461 atcgacgacg acgaacgggc ccttgttgcg gcggctgtgg cgatgcaccg cttcggcgat
     8521 cgcttctttg ccggtgccgg tctcgccttg cagcaggatc gtcgcctcgg tcggcgcagc
     8581 cttcgagagc agcgagaaca gccgcttcat gggctgcgtc gtgccgagca catcgccgaa
     8641 gcgatcgctt ggccactcgc cgatatcgac ggtcgtgtct accggctcga cgtccatctc
     8701 ggtgtgtttg cctaggcgca ggcgggcagc gccgctgagc acgaccgagc cgatgcgcgc
     8761 gccgccgtgc ttcgtgccgt tcgtcgtatc gagatcgcgg acctcgatgc cgtcgcgacg
     8821 tacgcggatc tcgaggtggt agcgcgagac ggtcgcgtcg gagagcacga gatcgttgtc
     8881 ggcgtgcgtg ccgaccatcg tcgtgccttc gtcgagcatg tgctctttgc cctggtcagg
     8941 gcccgcaacg acgcggaggc gaaacgaccg ctcgagcaga agcccgctcg cgtcgtcggt
     9001 caggagctga gtatggaccg aggtcacgtc gctaatcgtt tgtaccgcga cgacgcctca
     9061 ggaagaaagc gacgaggacc aagagcggca cggtcccccg atcgcctccg ccgctctcgc
     9121 agcagccgcc accggggtcc atgtacccgt tgcctccgtc gctggtctgt gcgtcgcccg
     9181 cggtcgttgc atcgggataa acggctgcat ccttgcggac tccggcgtcc ggcgggtcca
     9241 tgccctccca cagcgcgacc gagttgtagg tgatctcgcc ggcgacgacc tggcggcact
     9301 gcttgacggt ctggtagccg gtcttcttca cggtcacgca ggccaggcgc ggcgtcacgc
     9361 cgaggaagtc gaagacgccg gtcgaatcgg tcgtctgcat gcgcgtgtca tcgaggaaga
     9421 cctgcgcgcc gctgatcttg cgcggcggat cgttcgcgca gccgatcacg ttgtgcttgc
     9481 agacgaggcc gtacatctgg cccttcggca gctggccata cttgaacgcg aggtggttcg
     9541 cgacggtgcg ctcgacacca tcgctcgggt tcgagatcac gtcgccatcg agatagagcg
     9601 tcgagctcga gccgccgtcg agcgccagcg ccatccccgc tccgcgggcg acgagaaact
     9661 cggcgagctg cgggcacgtc acgccgatcg agcctgactg ccacccatcg acgaccgcga
     9721 tccacagcgt gcgcccatcg ggtgaaattg cgactgcggt ccgcggcgcg ggctggcacg
     9781 gcatgctcgt cgagtcgttg cagtcgaact gcgtgacggc cgcggactgc cgcacgagca
     9841 gcggtcggcc ggagatcacg ccttcggtgc cttcgggcag atccatcggc gtgatgaccg
     9901 cctcgggcgg gatgatgtcg gcgaccgtgc gctcgccctc gcgccggaag aggaacgtcg
     9961 cggtctgcgt gttgtcggcg gtctgcgacc acgggttgct cgcacccatc gcgaggccgc
    10021 gcggcacgta gccgttgacc gcgaatgcat caccgttgat cgcgagctgc gcgttcttgc
    10081 gcgtcgcgaa ctcggtcgtc gtgatgccgc gatcagactc ctgcgtggcg tatgccgcga
    10141 tctccgacga ggtcaggtcg atctgcacga ggcggatccg cgcgggaatt gcgttgtcgg
    10201 tccacgtctc gcgatgcatg cccggatgcg ggtcgcccga gaacgtcatc gtgaagccgg
    10261 cgaacgcggg cgtcgcgaat gcgcagacca gggcgagggc caacttcaca ggccgagcat
    10321 agccgagcta gttcgcgacg acgggcgttc ccgctttgac gcggacttgc acttgatgac
    10381 tgccggcttc gtcgaggacc gtgaccgagc gcgggcctac ttcgaggacg gcgcgtttca
    10441 ccttctgacc agctacttgc agcatcttgc cttcaccggc gtcgacgtag ccgagctcga
    10501 acttgtcggt catcggcttg cccttgacgg cgatggaatg ggtggcgcgg ccgccttgcg
    10561 tgacgaagtc gacagcgtac gagccggggc cgagcacgca ctcgacgggc ggcgtcatgt
    10621 ccttgcagtg cggcgcgggg gtgaccgtga tcttgccgtc gaggttcgct tcgaccgtca
    10681 gctttgcacc ggcgacgagc ttggcggact tcgtgatgcc cgtcgcgccg cgtcccggcg
    10741 tgaacgcgtc gatcaccgcg gggacgtgat ccggcatcgt cagcgtgaag cgctgcggct
    10801 tgccggcttc gacgagcact tcggccggcg tcgtcacacc agcgagcacc tggccgtttt
    10861 gcacgacctg cgcgccggga ggatccgatt cgatcttgat gcgcgcgagg tcttcggaga
    10921 cctgcatcgg cagcaccatg ttgagctcct tgccagggct cggaacattg acgctcgtcg
    10981 tttgatcgcg ataaccggcc ttcttgaaca cgacctgctg cgtgctgccg ggaggcagcg
    11041 tggtcacggt gaccggcgtc gtgccgatcg gcttgccatc gatctcgacg gtcgcgccgc
    11101 tcgggagcgt gtcgaggaga accgtcgctt ggcgtgctgc gagctcgaac gagacggcgg
    11161 tgtcgcccgc gcgcggctga acgaccgcgg tcttcggctc gtagccttcg agctttgcga
    11221 cgacgggata ggcgcgaccg acttcgagtg agcgcacgag tgtcccatcg gtcgcggtgc
    11281 cttcgttgcg gccttcgatc tcgatcgtcg cgcctttggg caccgtcgtg aaggtgacgt
    11341 tcgtcatctg cttgccggcg ccacccatcc gctgccatgc ggcgagcccg acgagcgcga
    11401 gcaccaagac gatcgcggcc gcgagcgcac cgatgagacc gcgcgacggg gcaggtccat
    11461 cgggagggcg acgcctctcg accggatgac ccgcggacgg caagcgacgc gacgcaccca
    11521 cggcgagccg gccaccagcg tcgatcttct cggtgaagaa gccgatctct tcgagctgga
    11581 tcttcggcgt ctcgagatcg aacacgcaca cggcttccgt cgcgacgccg ggcagcacgt
    11641 tgaacatgac cgtcgacgcc gagttcgcca tcaagtagag gccgagaccg gcgccacctg
    11701 ctttgcgatc gatctgctga tcggccggcg cgtgcagaca cttgtagagg tacttgagca
    11761 ccgtgctgcg ctcgagcgtg ccgaatgcat cgcgcacgct gacgacgaac tgatgaccgt
    11821 cgcacgcata ctggacgacg accttctgct cgacgcgcag cgagatccgc gtcttcgtcg
    11881 gaatctccga gaagatctgc ttgccctgct cgtcgacggg cgcgtcgtag agcgcgttca
    11941 tcagcatctc gtcgacgcac tgctcgacgg cctctcgata cttgcggcgc acgcccatca
    12001 gctcggcgaa ctcggagatc tgcgagatgc agagcgactt ctcttggtag tcgccgacga
    12061 gctggctgtg aacgagcgtg ccccacttga tgagcttctc gaggccgaaa atatcgccga
    12121 cgagaacgcg cgtcgccatc gcggagagct gctgcgagtt gaagtcttca gcgacgagca
    12181 ggccggagac gcgatcgctc gactgcatga tgtcgacgac ggccgcgagg ttcgtgcgag
    12241 gcagcacgca gatcacgcgc gtgtcgccga tcagacgcgg cagaatttcg gccgccgcgg
    12301 tttgcagctc gccgtcgaga tgcagcacga cgagcgcggc ttgcagctcg cctttgccga
    12361 gcgagtccat cgacgcatgg agatcgacag ctccgcctgc ggccttgagt gccgttgcta
    12421 gctgcttgcc gaacgccttg tccggcgaga gagcaatgat tctccgcgcg agcacccgat
    12481 caccatcgta gcgcagccgc tcgacgggcg acagttcagc gcacttatcg gcttacttac
    12541 gtaagcggct cagaaaagca cctgaccggt gacaccgaac gctacgtcga agccttcgta
    12601 cgagcggtag cggtggccaa ccaggtcaag gccgggaacg tggagcgggt tgacctcgtc
    12661 ggtgcccggg ttcacgaggt cgttgtcgtc gtcgggcgag tccttacctg cgtcggcgaa
    12721 cgtgatcagg tggtcggtct tccacgtgag atcgccgctg accgaaaacc ggacgtgtgg
    12781 gccgagctgc gcacgaaccg cgaacaggcc gtgggtctcg agatagttct cgacgttcga
    12841 gatgcccggg tagctgagcg gctgcacgtc cgcgcgcgtc ggatcggaat cgaggatcaa
    12901 cgggccggtg ccgcggctat cgccagcgta cgcgaacgcc tcccacatct cggtgtactc
    12961 gcggccctcg aagtggacgg tcgcgcgcgt gccgaaatcg aggctgatgc gcgtctgatc
    13021 gcggccctga tcgagcgcgt agagctcgag gccgaagcca aggcctgctt gctggctctt
    13081 ctggatgttc gacgcaccga agcccggatc ctggaacagc gcgtcgctct tcgctgcgag
    13141 cgggaccatc caccagaggt cgacccaggg ctcggcccag ccgagcttgc gcgcaaatga
    13201 cgtccagacc ttcagctcct gcacgccctg cgagacgccc gtgttggcgt tcggagccat
    13261 cggatcgaac ttcatgatct tgccgacggc gaggcgaatc tcggcgccga gcttccagat
    13321 cggcttggtc ggatcgtgcg cctgattcat cggcgcgatg ccgaggccga ggtgcacttg
    13381 gtcgagacct ttgcggctcg gcccgcggaa catgaggttg ccggcagcgg gcgtcgtcgg
    13441 gtcgcgcgcg tcgaagccct gcatcggcaa gagcccgtcg gtcacggtcg gcgagcctgc
    13501 gcgatcgact ccatcgtcga gctcgagctc gcgcacttgc gtgatgacga tcggcagcgc
    13561 ggcgtagaag aacgtgtcgc ggaagatgcc gacgtgaacg ctcggcgtga tggtgtgctt
    13621 gaactggtgg aacttcaggt cgtggaccgt cggcaccgcg gcgttcggat cgctccctgg
    13681 ccccagccgt tcgcgaatga gcgaggagct gtccagctcg taggcgtagt cgacctgacc
    13741 gtagatatcg accgacttct gcgcgtggtc cgaattcgga tcctcgggat cctgaccgcc
    13801 cggaacgatg tcggccggat tcgcgtacgc gtccacgctt gcgaggacga ggagcccaac
    13861 tgagaagacg ctccggcgca gcaccctgct tgaactatac acggctagct agctctcgcg
    13921 atcgcggagc ccgaactcct tcatcttcat ctgcaggctc ttgcggctga tcttgagcag
    13981 gcgtgcggcc tgggtcacgt tgccgccggt ctcgtcgagc gccttgacga tcagctcgcg
    14041 ctcgacacga gcggtctccg cgcggacgac ctctttgaga gatcccgaca gctcggcgtc
    14101 gtcgacgtca tcgcccagca tcggcacggg ttgcgactgc gaggccggct cctgcccagc
    14161 cgatctgagc tgcagatcct ggcgctcgat gcggtcgcct ttggtgaaga ggatcgtgcg
    14221 ctcgaggacg ttctcgagct cgcggatgtt gccgggccag ctgtgcgtct cgagggcggc
    14281 tagcgcctcg tcggagatgc cggtgatgct cttcttcagg cgctcgttga acttcttgat
    14341 gatgtggccg acgagcagcg ggatgtcgcc cgtgcgcttg cgcagcggtg gaatctgcag
    14401 cggcacgacg ttgaggcggt agaacagatc ctcgcggaag ttgcccttct ggatctcc
//
LOCUS       DATLBZ010000573         2919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3388854, whole genome shotgun sequence.
ACCESSION   DATLBZ010000573 DATLBZ010000000
VERSION     DATLBZ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3388854"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VMZ53_28455"
     CDS             complement(<1..1027)
                     /locus_tag="VMZ53_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HUS32479.1"
                     /translation="MSSPSVEELKALLDRRIVVLDGAMGTMVQGLGLADEAVWRGDRF
                     KNHSHALKGCNDLLVLTQPQAIEDIHLQFLQAGADIVETDTFTATSLALADYGLESVV
                     RDLNIAAAQVARRAAERAMAADGKPRWVAGSIGPTTKSASLSPDVNDPGARGVTFREL
                     VESFGEQARALIEGGVDLLLTETHIDTLNCKAALFAFEELFASGVRRVPVIASVTIPD
                     KSGRTLSGQTIEAFFNSISHTNLFAVSINCALGADDMRPHVQELARIAALPVACYPNA
                     GLPNEFGGYDDTPEHMAGVLGAFAREGMLNIVGGCCGTRPEHIKAIRAAVEGVARRTI
                     ARPAKHMR"
     gene            complement(1061..1750)
                     /locus_tag="VMZ53_28460"
     CDS             complement(1061..1750)
                     /locus_tag="VMZ53_28460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32480.1"
                     /translation="MRRAALSVLALAGCDVVFRVDHVPDAPAGDAVTADALSICTLPF
                     IDDTFDDGTPCAPWGSPRMQVGGTVTESNGSIIVQPAPNMIGSRGGCDAMGAVPFGTG
                     GVVAEVSHAMGGSDAEFTLLASGSLQLAVAVANNQIVFQNDIGTIQYGAAILYVPTAM
                     RFWRIRRFNDVVITGEYSADGVAWTELGRQTVALAPTTANFGLLAGLNNNRPAQSAPD
                     RSVYDRFVVCP"
     gene            1792..>2919
                     /locus_tag="VMZ53_28465"
     CDS             1792..>2919
                     /locus_tag="VMZ53_28465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32481.1"
                     /translation="MELADRQLGRYHLLRLLGFGGMGEVYLASAKGAAGFEKLVTVKV
                     LRRQVSQNPDRVRDLLREAFIGVRLDHENIVQVLDLGEHEGNYFLVMEYVRGFSLREL
                     IAFHSEQKELFPTRPLVHALRCITEALDYLHRVRGPHKKHLGLIHRDISPSNILLGAE
                     GRIKLSDFGVAGMARDAATAGTVIGKPRYLPPEARHGEAATQGWDLYALGVVLHSALA
                     ADPDVEKGGTKIGARLAPLERTDVPAPLLELVAGATARDPANRLGDAATFRKLLDEAE
                     PKQADDAEFWRGWLQGLYRREPFISRYGVLPYVEDLVPELETKQAAQPPSVAYVETVV
                     VDTQKPIRFGLSPALGVQAARTQGERVAAWLKTRFDREVRPV"
BASE COUNT          488 a          917 c         1011 g          503 t
ORIGIN      
        1 ggcgcatgtg cttcgccggc cttgcgatcg tgcggcgagc gacgccttcg acggcggcgc
       61 ggattgcctt gatgtgctcg gggcgtgtgc cgcagcaacc gccgacgatg ttgagcatgc
      121 cctcgcgggc gaatgcaccg aggacgccgg ccatgtgctc gggggtgtcg tcgtaaccgc
      181 cgaactcgtt cgggaggccc gcgttcggat aacacgcgac gggaagggcg gcgatgcgcg
      241 cgagctcttg cacgtgcggg cgcatgtcgt cggcgccgag ggcgcagttg atgctgaccg
      301 cgaacagatt cgtgtggctg atcgagttga agaacgcctc gatcgtctga cccgacagtg
      361 tgcggcccga tttgtcggga atcgtcaccg acgcgatcac gggcacgcgg cgcacgccgg
      421 atgcgaatag ctcttcgaat gcgaacagcg cggctttgca gttcagcgta tcgatgtgcg
      481 tctcggtcag cagcagatcg acgccgcctt cgatgagcgc gcgtgcctgc tcgccaaacg
      541 attcgacgag ctcgcggaac gtgaccccgc gtgcacccgg gtcgttgacg tcgggggaca
      601 gcgacgcgga tttcgtcgtc gggccgatcg agccggcgac ccagcgtggc ttgccgtcgg
      661 cggccatcgc gcgctccgcg gctcgacgtg cgacctgagc ggctgcgatg ttgagatcgc
      721 gaacgacgga ctcgaggccg tagtcggcga gggcgagcga tgttgcggtg aacgtgtcgg
      781 tctcgacgat gtcggcaccg gcttggagaa attgcaggtg aatgtcttcg atcgcctgcg
      841 gctgcgtgag cacgagcagg tcgttgcagc ccttcagtgc atggctgtgg ttcttgaacc
      901 gatcgccgcg ccatactgct tcgtctgcga ggccgaggcc ttggaccatc gtgcccatcg
      961 ctccgtcgag aacgacgatg cggcgatcga gcagggcctt cagctcttcg acggacgggg
     1021 acgacattgg gccctgatta ccaccctttg ggcggcggac ctaggggcac acgacgaagc
     1081 ggtcgtacac ggagcgatcc ggcgcactct gtgcgggccg gttgttgttc aagcccgcga
     1141 gaaggccgaa gttggcggtg gtcggtgcga gagcgacggt ttgccggccg agctcggtcc
     1201 atgcgacgcc atcggccgaa tactcaccgg tgatcaccac gtcgttgaag cgacggatcc
     1261 gccagaagcg catcgcggtc ggcacgtaga ggatcgcggc gccgtactgg atcgtgccga
     1321 tgtcgttctg aaacacgatc tggttgttcg cgaccgcgac ggcaagctgc agcgagccgc
     1381 tggcgagcag cgtgaactcg gcgtccgatc ctcccatcgc gtgcgagacc tctgcaacga
     1441 cgccccccgt gccgaatgga acagccccca tcgcatcgca gccgccccgc gagccgatca
     1501 tgtttggcgc aggttgcacg atgatcgagc cgttcgactc tgtcaccgtt ccaccgacct
     1561 gcatcctcgg gctgccccaa ggtgcacatg gggtgccgtc gtcgaaggtg tcgtcgataa
     1621 agggcagcgt gcagatcgag agagcatcgg cggtcacggc atcgccggcg ggcgcatcgg
     1681 gcacatgatc gacgcgaaac accacatcgc agccggcaag cgccaagact gatagcgccg
     1741 ctctacgcat cgagtcgatc atagcgcggg acggtgtagg atcgaaaacc ggtggagctc
     1801 gcggatcggc agcttgggcg ctaccacctc cttcgcctgc tcggctttgg aggcatgggc
     1861 gaggtgtatc tggcgagtgc gaagggcgcc gcgggcttcg agaagctcgt gacggtcaaa
     1921 gtccttcgcc ggcaggtcag ccagaacccg gaccgcgtgc gcgaccttct gcgcgaggcc
     1981 ttcatcgggg ttcgcctcga tcacgagaac atcgtccagg tcctcgatct cggcgagcac
     2041 gaggggaact acttcctcgt catggagtac gtgcgcggct tctcgctgcg cgagctcatc
     2101 gcatttcaca gcgagcagaa agagctgttt ccgacccggc cgctcgtgca cgcgcttcgc
     2161 tgcatcacgg aggcgctcga ctacttgcat cgcgtgcgcg gtccgcacaa gaagcacctc
     2221 gggctcattc atcgcgacat cagcccgtcg aatattctgc tcggcgccga gggacgcatc
     2281 aagctgtcgg acttcggtgt ggccggcatg gcgcgcgatg cagcgaccgc gggcacggtg
     2341 atcggtaagc cgcgatatct gccgccggaa gcgcgtcatg gcgaagctgc gacgcagggc
     2401 tgggatctct acgcgctcgg cgtcgtgctg cactcggcgc tcgctgctga tccagatgtc
     2461 gagaagggcg gcacgaagat cggcgcgcgg ctcgctcctc tcgagcgcac ggatgttcca
     2521 gcgccgttgc tcgagctcgt cgcaggagcg actgcgcgcg atcctgcgaa tcgcctcggc
     2581 gatgcggcca cgtttcgcaa gctcctcgac gaagcggaac cgaagcaggc ggacgacgcg
     2641 gaattctggc gcggctggct gcaaggcctc tatcggcgcg agccgttcat ctcgcgctac
     2701 ggcgtgctgc cgtacgtcga agatctcgtg cccgagctcg agacgaagca agccgcgcag
     2761 ccgccgtcgg tggcgtacgt cgagacggtt gtcgtcgata cgcagaagcc gatccgattc
     2821 gggctgtcgc ccgcgctcgg cgtgcaggcc gcacgcacgc aaggcgagcg agtcgcggcg
     2881 tggctgaaga cgcgcttcga tcgcgaggtg cggccggtc
//
LOCUS       DATLBZ010000574        12614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7829522, whole genome shotgun sequence.
ACCESSION   DATLBZ010000574 DATLBZ010000000
VERSION     DATLBZ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12614
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7829522"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VMZ53_28470"
     CDS             <1..212
                     /locus_tag="VMZ53_28470"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HUS32482.1"
                     /translation="GWLLLEQAVIAETALGKLTGEHPDKAFYTGKTYAARYFALNVLP
                     GVVAKAQLIAREDRSAVDMPIDAFA"
     gene            212..1321
                     /locus_tag="VMZ53_28475"
     CDS             212..1321
                     /locus_tag="VMZ53_28475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32483.1"
                     /translation="MRFAVGVCCLVACGGTTPPQQSPVVANRPWSAAPPTCEEVGIIL
                     RGANEDRSEAAGPAKERAIAEACTRDNWSQLVIACVAGTTDAHACLVQLTQAQHAALD
                     NRLAAWAAQFGGDAGDSAPAATCFDALHAIELFDPPINDTSPERQWQINVRQRVLVDM
                     CTNDPWTDEVNACLRDAASEAATGACVATLDPKVHAKLVSLRELAKAIEALRKTPAKI
                     DCKKVAEHHHSDARTKDALKALKPAERKQLLASARKTMADACTNEGWDEPTRACMIAD
                     GGEETCFGTVRWGSVTPGIPMIAIPECGDYATAVRDLQVCSAAPADTRAAMKQAFEEV
                     ERASMNVPADQKGALATACKTAADAVVQVRAAYGC"
     gene            complement(1318..1662)
                     /locus_tag="VMZ53_28480"
     CDS             complement(1318..1662)
                     /locus_tag="VMZ53_28480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32484.1"
                     /translation="MRRFVCLLVLAACQPKQPLQASYFHAKPVEYASCQDTVTCYARC
                     SPVTEPCMQACDRNALPDDALHARDLTSCLQNTRCRDESCTREQCGIQIDTCNNARQQ
                     MPGQYQTQGGPI"
     gene            1818..5306
                     /locus_tag="VMZ53_28485"
     CDS             1818..5306
                     /locus_tag="VMZ53_28485"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HUS32485.1"
                     /translation="MRTSPATRLATLLLATGAAFLGACSDELEVTPLYNPANGRVVIQ
                     VNQDIGGKQLYVQVRRGKFGVLDCKQMAQNLTAVDDLSGSRIDGPFVDPQLTKPFYGG
                     AEWMNPTAQMIAQAEAGTDSIIDVCLMDGSDVVVQMERDLFRAWDDARKQGLGGKADN
                     PESGEQFINSPVAYGERCVAELGEIPFFEKEADGKYSTYDCINSTEIPLTVTKADGSV
                     DKPREGTVNQCDNPQYIYSLCEAGPRVAQRTNEQGTRWVLLCRKSKGGYASNQYNDIA
                     MIGHNPFTGKTCFFQNALYSKTDGGHVPHPADKEKSQNLWSGVHGGLGSGIECSQCHD
                     ADPFIHTPWIDGAKDAQGRPIVPKMGIDSDLAIGANDTPYSLINAKGQGWKYERQIVS
                     PEASACLKCHRMGSGRWTESWISRLGGTDTAWTGITTAAYNQSAHKYWMPPDTAMDSE
                     AIWSASVFKTALDFIEKCGKNSSDPTCIWKDIPTKLASDTAGGALRNKVTLADDELAK
                     QATTIIGMNKNTPSQICAECHAPNQTTLRDWQEKTDTAMSSCLKNVDDGGTQTTEKKE
                     NVSIGKDELKTFGPYEVALGGKIEVSMSGTGDADLYVKRGGEPTVDSYDCRPFSREPN
                     EECKATQFNANGPAKFYVGVNGFADSSKITITVTYGKPSASTPKTKDIVDCLRLEPGR
                     SDSPFVPSKVGIYAAAAHLGFFQGVFQDAYSDAGQDAWAIEYGKFKNRSSMPKGNHPR
                     LTQAELDIVAEWFARGLPRLTTYIAPDTGPTTCTASITPAVQTHVTAMSTQGWNAVNR
                     NNNISMYGCGSSTNPRECLTSLPDATTKAYGGGWAQLGKLRVLRELSFNTYYWMRSSA
                     DGRFVANGATGGDGAVISDLQSNKDMKVKAAYDPGFFPDNRGWMFQGTPIGGDFCTTG
                     LLVRNPDRIDFSEGECSHVEGVSLYQHLGSALGGGDYFAINSQFTSDNPSGVVNRDPS
                     AAFAATAEMKFTAMMFDGAHYVGKPSVSTKSPFEGDSVLSPSTKLVISRFGNENEHLG
                     YVLRKVDATKSGSSYTVSTPEVARYCMKGAKPAISFDEKYFVVHHYIGPNDWQELGYS
                     SATDPNFQQLVQKGASNIYIVNIVTGAKARITNMKPGQYAIFPHWRSDGWIYFLVRDK
                     NTNKEYAVASDAALVW"
     gene            5316..7001
                     /locus_tag="VMZ53_28490"
     CDS             5316..7001
                     /locus_tag="VMZ53_28490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HUS32486.1"
                     /translation="MRRAVVLLALIACGDEPDKTAVCHFGEATSAATIQDRKIIGGAA
                     PFDADTSLAARDEELRTSIAARRAAAWNAVSKVLAPTPLVETKLAQNFGGVQPKVPAW
                     HTWFSREDFDRVFKKVYRDLGPAARRARAPLDAATIDAGFTWNTTALDGLPDWDQQRY
                     DDYVAAVTTQELAQGLGGISRVGYSTGAMRQLVHSYKQQYGCRVNAAPDAFDAQPVRP
                     GTPTRAVEQMTLGECSWQVLGPFAAGAGKVTASSMGDGDVDLYIRKGAAPTPDEHDCK
                     SAGGSSTETCSVDGGGMFYVAVFGATASAATVTVEYTTEDVAAPTCLEAEMPRDGVLV
                     KADWRRVLTGENLPIYDTSAARMRERLNGDLDWHDGDGSQNPDSSAIYTATLPNGNTF
                     RMPALHIMTKELDHWLWITLWYSSSPDSDFGADRPAAISALPGPWKNYKMCVASAYLE
                     GDADPRGGQAGSLGDALEAVHGGVGSPSWCSNPYLEKGGGNVGTNCIGCHQHGGTDIT
                     PEDILATLPHNGSTRVRNNFFTDYLWAIKGGQGEDLSALVQAEVDYWDANDPP"
     gene            7011..9782
                     /locus_tag="VMZ53_28495"
     CDS             7011..9782
                     /locus_tag="VMZ53_28495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32487.1"
                     /translation="MRRALLLIVLAGCGRIGFDEVCGASGPCVDVRINDGATYARAPR
                     LTVKVTTSTDDAGQEAQFSEGPCELAADGVWHPHADGTPVPVLVEPVDGAKQVCVWVR
                     DSTGVGFASATIQYETLNLPQIDVLDVVALDATTARATWTVSDVEGLAANPMDFEIRT
                     GTDWMPLATEIGGGVGATTSATGAFDFAMPQGPFMVRATVRDRAGNRGVPAISDIIGA
                     PNWHVYAGTDDNGIGESARALLLEFAENTAYMFAVDPQTMDAYVVDRETGVIRVDGAT
                     GDSTLLIGIGVNNLPDDGPLPVDAKVPLDPALHIGPDGKLYVSAGTSSVGSSSRIYQI
                     DPQTLHCKRYIGGGMGNDATATPATVFVSKQVWTFDEDGALYFLTACVPNSTTAPFRM
                     RFLRAARNADGSAGALTVLAGNCATPGGLIDGLDATTVPLPGFANFPGIGTISVLDQG
                     RLIYVGSNGPVAFKILDGRIYSSDLPVGSEGTLALDAVTRTVLVGHGAVEEHMAGAPG
                     TSDLQIRTRLKEDGGTRCADDNVNAVDACVEVGYGIQSTQARVYFADGTGINNHRAYR
                     VRFIDRAGRLQTYVGGHGFYGEGLHRRSVRSRMGGIVYKPASASNGAAFPAGLYFTDP
                     LDLVMARIDPATDRASVVWGNRSGAPRTYNPGETLGTDKGLGFNYGAGNLAAMTFDTA
                     GLPMLRYGTDEDRAGLLTADAMRRAQPLMTGTQRWETMANGADPTTAGLWPYGALNNL
                     TYYAGNLFVLGGYTNYNATSVLKRFDFVNGTTTRLMGLGADAFTPDGATPATASLSGS
                     CHDGISKCFTQYNAADDRLYFSEDNRLRYLTTPLGPTQSLGTLFIAPTDVRNFTFRPD
                     GSQIYYANGGLRCHDITSGSAACNDTVLGPPALLGTIGLGPDQLTWIDNNTLLISTHT
                     GRILQYTVQ"
     gene            9976..>12614
                     /locus_tag="VMZ53_28500"
     CDS             9976..>12614
                     /locus_tag="VMZ53_28500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32488.1"
                     /translation="MDLELTVVQRAKPELGYIAGLAVGDELIVAVGGTSRHAPTVLAS
                     SDARHFEPRKTPRQLGLRDVLVVGSAIWTCGEYGQLAVSRDRGESWKVIDTGCDSCLF
                     GLALAADGAVWVCGADGYVARIDDPQKRLERIQLDTTTRLTAIYCVRDEVIALGLDGV
                     MYRWRAGAVTKFVTGATKQLTALAITKAGTWLVIGDGGFVARSPDGAWFSRVKAGVEH
                     DLESIAPLADGRVVMVGDRGTIVLSTDDGRTWAPVESDVSAHLWSARRFGAGLLIGGD
                     DGLVLQLAPQGDRAWNDRIDIFGNVKPLDGVLALGPAGFLGRLPAYIATLGCEAAKDA
                     TVAPSTDAFARIYGVALPSEVDALFAATRGRDCFDELHLDATLIADVGDENVFERVLA
                     SGATDAFCGVFCIGAQSSGGSYHMELYEWDGPRQVLHYDRSTRAFSGVIADSLESLVY
                     LAALVRAEKRRLISDEVFTNGLRKLHGKVAPTFDVGEGFVKLDAKRRDTEFFFYRSRW
                     ICALLDGRVEISEIPPLFMADFNQIVPNEQLSARFEACERIIPTALYSMWRAFVFDEP
                     ELARYLEIGSRHKSRLVRDAAKLIAELRNGRNTLGTIGDVRARLAAFRALELDPRRAM
                     RRTEIESTPRDRWTDLAWRWVDDGVAQRALLAKLVEEPALAAQVEALDKLRWLPDVER
                     ERAVQRLAKQLAPELEAILAGSIVRGDDLSDALGRVDHVDEIKARAVAAHERVQAALA
                     MTTRALALDPHDDTTEYTHSVLLLEAGKAEELVARWPSLSSQNQEALIDQIAPALNDA
                     CVQAHAEKAFDVAVRLAHHAQQFASAHPYLYHSAACAFAAVGDYAGAFEQVKLAIEHN
                     AERVADMEVDSDLGPLLDWPEFKA"
BASE COUNT         2311 a         4059 c         4187 g         2057 t
ORIGIN      
        1 tcggctggct gctcctcgag caagccgtga tcgccgagac cgcactcggc aagttgacgg
       61 gcgagcaccc cgacaaggcg ttctacaccg gcaagacgta cgcggcccgt tacttcgcgc
      121 tcaacgtcct gccgggcgtc gtggcaaaag cgcagctgat cgcgcgcgaa gatcgctccg
      181 ccgtcgacat gccgatcgac gcgttcgcct gatgaggttt gcggtcggcg tttgttgtct
      241 cgtcgcatgc ggtgggacaa caccgccgca gcagtctccc gtcgtggcga accgtccgtg
      301 gtctgccgca ccgccgacct gcgaggaagt cggcatcatc ctgcgcggcg cgaacgaaga
      361 tcgcagcgag gcagcgggcc cggcgaagga gagagcgatc gcggaggcgt gcacccgcga
      421 taactggtcg cagcttgtca tcgcatgcgt cgccggaacg accgacgcgc acgcctgcct
      481 cgtgcagctc acgcaggcgc agcacgcggc tctcgacaac cgcctcgcgg cctgggcggc
      541 gcaattcggc ggtgatgctg gcgacagcgc acccgctgca acctgcttcg acgcactcca
      601 cgcgatcgag ctgttcgacc cgccgatcaa cgacacgtcg cccgagcgcc agtggcagat
      661 caacgtccgc cagcgcgtcc tcgtcgacat gtgcaccaac gatccgtgga cggacgaggt
      721 caacgcctgc ttgcgcgatg ctgcgagcga agccgctacc ggcgcgtgtg ttgccacgct
      781 cgatcccaag gtgcacgcaa agctcgtctc gcttcgcgag ctcgcaaagg cgatcgaggc
      841 gctgcgcaag acaccggcga agatcgactg caagaaggtc gcggaacatc accacagcga
      901 cgctcgcacc aaggatgcgc tgaaggcgct gaagcccgcc gagcgcaagc agctcctcgc
      961 gagtgctcgc aagacgatgg cggacgcttg cacgaacgaa ggctgggacg aaccgacgcg
     1021 cgcctgcatg atcgccgacg gtggcgagga gacgtgcttt ggcacggtgc gctggggctc
     1081 cgtgacgccg ggcattccga tgatcgcgat ccccgagtgc ggcgactacg caaccgcagt
     1141 gcgcgacctc caggtctgct cggctgcgcc ggctgacacg cgcgccgcga tgaagcaagc
     1201 gttcgaagag gtcgagcgcg cgtcgatgaa cgtgccggct gatcagaagg gcgcactcgc
     1261 gactgcgtgc aagacggcgg cggacgcggt cgtgcaggtt cgcgctgcgt acggctgcta
     1321 gatcggcccg ccctgcgtct gatactgacc gggcatctgc tggcgtgcgt tgttgcaggt
     1381 gtcgatctgg atgccgcatt gctcgcgcgt gcagctctcg tcgcggcacc gagtgttctg
     1441 caggcagctc gtcagatcgc gtgcgtgcag cgcgtcgtcg ggaagtgcgt tgcgatcgca
     1501 cgcctgcatg cacggctcgg tgactggact gcagcgcgcg tagcaggtga cggtgtcctg
     1561 acagctcgcg tactcgacgg gcttggcgtg gaagtagctg gcctgcagcg gctgtttcgg
     1621 ctggcacgcg gccaggacga gcaagcagac gaagcgccgc atgaccgctc ctatctcgcg
     1681 aaaagcgctg ttcgtgcaag ggaatgcatt cggaggtgac ccctggcttg tccggctacc
     1741 ggcctagccg atcgatggat ctcgctggat tcatacgcgc cagatctggc tctgaagttg
     1801 ccaactgtcc ctgcatcatg cggacttcgc cggccacccg actagccacc ctcctgctcg
     1861 ccacaggcgc agccttcctc ggcgcgtgca gcgacgagct cgaggtgacg cctctgtaca
     1921 acccggcgaa cggtcgcgtc gtgatccagg tcaaccagga catcggcggc aagcagctgt
     1981 acgtccaggt tcgccgcggc aagttcggcg tcctcgactg caagcagatg gcgcagaacc
     2041 tgacggccgt tgacgacctg tccggctcgc gcatcgatgg tccgttcgtc gacccgcagc
     2101 tgacgaagcc gttctacggc ggcgccgaat ggatgaatcc gaccgcgcag atgatcgctc
     2161 aggccgaagc cggcaccgac tcgatcatcg acgtgtgcct catggatggc agcgacgtgg
     2221 tcgtgcagat ggagcgcgat ctgttccgcg cgtgggacga cgctcgcaag cagggactcg
     2281 gtggcaaggc cgacaacccg gagagcggcg agcagttcat caacagcccg gtcgcgtacg
     2341 gcgagcggtg cgtcgccgag ctcggtgaga ttccattctt cgagaaagaa gcggacggca
     2401 agtactcgac gtacgactgc atcaactcga ccgagatccc gctgacggtg acgaaggccg
     2461 atggctccgt cgacaagccg cgcgaaggca cggtcaacca gtgcgacaac ccgcagtaca
     2521 tctattcgct ctgtgaggcc ggcccgcgcg tcgcgcagcg cacgaacgag caaggcacgc
     2581 gttgggtgct cctctgccgc aagagcaagg gcggctacgc gtcgaaccag tacaacgaca
     2641 tcgcgatgat cgggcacaac ccgttcaccg gtaagacgtg cttcttccag aacgcgcttt
     2701 actcgaagac cgatggcggt cacgttccgc acccggctga caaagagaag tcgcagaacc
     2761 tgtggagcgg cgtgcacggt ggcctcggct cgggcatcga atgctcgcag tgccacgacg
     2821 ccgatccgtt cattcatacg ccgtggatcg atggcgcgaa ggacgcgcag ggtcgcccga
     2881 tcgtcccgaa gatgggcatc gactcggatc tcgcgatcgg cgcgaacgac acgccgtact
     2941 cgctcatcaa cgcgaagggt cagggctgga agtacgaacg ccagatcgtg tcgcccgaag
     3001 cgagcgcgtg cctcaagtgc catcgcatgg gctcgggccg ctggacggaa tcgtggatca
     3061 gccgcctcgg cggtacggac accgcgtgga ccggtatcac gacggccgcg tacaaccagt
     3121 cggcgcacaa gtactggatg ccgccggata ccgcgatgga cagcgaagcg atctggtcgg
     3181 cgtcggtgtt caagaccgcg ctcgacttca tcgagaagtg cggcaagaac agctcggatc
     3241 cgacctgcat ctggaaggac atcccgacga agctcgcgtc ggacaccgcg ggtggcgcgc
     3301 tccgcaacaa ggtgacgctc gcggatgacg agctcgccaa gcaggcgacg acgatcatcg
     3361 gcatgaacaa gaacactccg tcgcagatct gcgccgagtg ccatgcgccg aaccagacga
     3421 cgctgcgcga ctggcaagag aagacggaca ctgcaatgtc gtcgtgcctc aagaacgtcg
     3481 atgacggtgg cacgcagacg accgagaaga aggagaacgt ctcgatcggc aaagacgagc
     3541 tcaagacgtt tggcccgtac gaagtcgcac tcggcggcaa gatcgaagtg tcgatgtcgg
     3601 gcaccggcga cgcggatctc tacgtgaagc gcggcggcga gccgaccgtt gattcgtatg
     3661 actgccgccc gttcagccgc gagccgaacg aggagtgcaa ggccacgcag ttcaacgcga
     3721 acggccccgc gaagttctac gtcggtgtga acggcttcgc agacagctcg aagatcacga
     3781 tcaccgtcac gtacggcaag ccgtcggcca gcacgccgaa gacgaaggac atcgtcgact
     3841 gcctccgcct cgagccgggt cgctctgact cgccgttcgt gccgagcaag gtcggcatct
     3901 acgcagcggc tgcgcacctc ggcttcttcc agggcgtgtt ccaggatgcg tactccgatg
     3961 ctggccagga cgcctgggcg atcgagtacg gcaagttcaa gaaccgctcg tcgatgccga
     4021 agggcaacca cccgcgcctg acgcaggcgg agctcgacat cgttgccgag tggttcgcgc
     4081 gtggcctgcc gcgcctcacg acgtacatcg cgccggacac cggtccgacg acgtgcacgg
     4141 cttcgattac tccggccgtg cagacgcacg tcacggcgat gtcgacgcag ggttggaacg
     4201 cggtcaaccg caacaacaac atcagcatgt acggctgcgg ttcgtcgacg aacccgcgtg
     4261 agtgcctcac gagcctgccc gatgcgacga cgaaggcgta cggcggtggc tgggcgcagc
     4321 tcggcaagct ccgcgtgctt cgcgagcttt cgttcaacac gtactactgg atgcgcagct
     4381 cggccgacgg acgcttcgtc gcgaacggcg cgacgggtgg cgacggcgcg gtcattagcg
     4441 acctccagtc gaacaaggac atgaaggtga aggcggcgta cgacccgggc ttcttcccgg
     4501 ataaccgcgg ctggatgttc caaggcacgc cgatcggtgg cgacttctgc acgacgggcc
     4561 tgcttgtccg taacccggac cgtatcgact tctccgaggg cgagtgcagc cacgtcgaag
     4621 gtgtcagcct ctatcagcac ctcggctcgg cacttggcgg cggtgactac ttcgccatca
     4681 acagccagtt cacgagtgac aacccgtcgg gcgtcgtcaa ccgcgacccg agcgctgcgt
     4741 tcgcggcgac ggcggagatg aagttcacgg cgatgatgtt cgacggcgcg cactacgtcg
     4801 gtaagccgag cgtgtcgacg aagagcccgt tcgaaggtga cagcgtgctc tcgccgtcga
     4861 cgaagctcgt catcagccgc ttcggcaacg agaacgagca cctcggttac gtcctccgca
     4921 aggtcgacgc gacgaagtcg ggcagctcgt acacggtctc gacgccggaa gtcgcgcgct
     4981 actgcatgaa gggcgcgaag ccggctatct cgttcgacga gaagtacttc gtggtccacc
     5041 actacatcgg cccgaacgac tggcaggagc tcggctactc gtcggcaacg gatccgaact
     5101 tccagcagct ggtccagaag ggcgcctcga acatctacat cgtgaacatc gtcacgggtg
     5161 ccaaggcgcg gatcacgaac atgaagcccg gccagtacgc gattttccca cactggcgct
     5221 cggacggttg gatctacttc ctggtgcgtg acaagaacac caacaaggaa tacgcggtcg
     5281 ccagcgacgc tgctctcgtc tggtaacctg gctgcgtgcg ccgcgcggtc gtcctgttag
     5341 ctctcatcgc ctgcggtgac gagcccgaca agacggccgt ttgtcatttc ggagaagcga
     5401 cgagcgcagc gacgattcag gatcgcaaga tcatcggcgg cgccgcgccg tttgacgcgg
     5461 acacgagcct tgccgcgcgc gacgaagagc tgagaacgtc gatcgccgcg cgccgtgcag
     5521 cagcgtggaa tgccgtgagc aaggtgctcg cgccgacgcc gctcgtcgag acaaagctcg
     5581 cgcagaattt tggcggcgtt cagccgaagg ttccggcgtg gcacacgtgg ttctctcgcg
     5641 aagatttcga ccgcgtgttc aagaaggtct atcgcgatct cggtcccgcc gctcgtcgtg
     5701 cacgtgcgcc gctcgatgct gcaacgatcg atgctgggtt tacctggaac acgacggcgc
     5761 tcgacggcct gcccgattgg gatcagcagc gctacgacga ttacgtcgcc gccgtcacga
     5821 cgcaggagct ggcacagggc ctcggcggta tcagccgcgt cggctattcg accggcgcga
     5881 tgcgccagct cgttcacagc tacaagcagc agtatggctg tcgcgtgaat gcggcgcccg
     5941 atgcgttcga cgcgcagccc gttcgtccag gcacgccgac gcgcgccgtc gagcagatga
     6001 cactcggcga gtgcagctgg caggtgctcg gtccgtttgc ggcgggcgcc ggcaaggtca
     6061 ccgcatcgtc gatgggcgac ggcgacgtcg atctctacat tcgcaaaggc gccgcaccga
     6121 cgcctgacga gcacgactgc aagagcgcgg gcggctcttc gacggagacg tgcagcgtcg
     6181 acggtggcgg gatgttctac gtcgcggtgt tcggcgcaac tgcgagcgcg gcgacggtca
     6241 ccgtcgagta cacgacggaa gacgtggcgg caccgacgtg cctcgaggcc gagatgccgc
     6301 gagatggcgt gctcgtgaaa gcggattggc ggcgcgtgct gaccggcgag aacctgccga
     6361 tctacgacac gtctgcagcg cggatgcgcg agcggctcaa cggcgatctc gactggcacg
     6421 acggcgacgg cagccagaat cccgactcgt cggcgatcta cacggcgacg ctgccgaacg
     6481 gcaacacgtt ccgcatgccc gcgctccaca tcatgacgaa ggagctcgat cactggctgt
     6541 ggatcacgct gtggtactcg tcgtcgcccg acagcgactt cggcgccgat cgtccagcgg
     6601 cgatcagtgc gctgcccgga ccgtggaaga actacaagat gtgcgtcgct tccgcgtatc
     6661 tcgaaggtga tgccgatccg cgcggcggcc aagccggctc gctcggtgac gcgctcgaag
     6721 cagtgcatgg tggcgttggc tcgccgagct ggtgcagcaa tccgtatctc gagaagggcg
     6781 gcggcaacgt cggcacgaac tgcatcggtt gtcatcagca cggtggcacg gacataacgc
     6841 ccgaggacat tctcgcgacg ctgccccaca acggctcgac gcgcgtccgc aacaacttct
     6901 tcacggacta cttgtgggca atcaagggcg ggcagggcga ggacctctcc gcgctcgttc
     6961 aggccgaagt cgattactgg gacgccaacg atccgccgta gaatgaacgg gtgaggaggg
     7021 cgctgctgct gatcgtgctc gcaggctgcg ggcgcatcgg cttcgacgag gtgtgcggcg
     7081 cgagtgggcc gtgcgtcgac gtgcgcatca acgatggtgc gacgtacgcg cgagcgccgc
     7141 ggctgacggt gaaggtcacg acgtcgaccg acgacgcggg gcaagaggct cagttcagcg
     7201 aaggcccatg cgagctcgcc gcggatggag tgtggcatcc gcatgccgat ggcactccgg
     7261 tgccagttct cgtcgagccg gtcgatggtg cgaagcaagt ctgcgtctgg gtgcgcgact
     7321 cgacaggcgt cgggtttgcg agcgcgacga tccaatacga gacgttgaac ctgccgcaga
     7381 tcgatgtgct cgatgtcgtg gctctcgacg cgaccaccgc gcgcgcgacg tggaccgtca
     7441 gcgatgtcga aggcctcgcc gcgaatccca tggatttcga gattcgaacc ggcaccgact
     7501 ggatgccgct cgccactgaa atcggcggcg gagttggggc caccacatca gcgacgggcg
     7561 cgttcgactt cgcgatgccg cagggcccgt tcatggtgcg cgcgacggtc cgcgatcgcg
     7621 cagggaatcg cggcgtgcct gcgatctcgg acatcatcgg cgcaccgaac tggcacgtgt
     7681 acgctggaac cgacgacaac ggcatcggtg aatcggcgcg cgctctcctg ctcgagtttg
     7741 cggagaacac ggcgtacatg ttcgcggtcg atccgcaaac gatggatgcg tacgtggtcg
     7801 accgcgagac cggcgtgatc cgagtcgatg gtgcgacggg cgactcgacg ttgctcatcg
     7861 gcatcggcgt gaacaatctg cccgacgatg ggccgcttcc cgtcgatgcg aaggtgccgc
     7921 tcgaccctgc gttgcacatc ggaccggatg gcaagctgta cgtcagtgcc ggcacgtcgt
     7981 cggtgggcag ctcctcgagg atctatcaga tcgatccgca gacgctgcac tgcaagcggt
     8041 acatcggtgg tggcatgggc aatgacgcga ccgcaacgcc tgcgacggtg ttcgtgtcga
     8101 aacaagtatg gacattcgac gaggacggcg cgctgtactt cttgacggcg tgcgtcccca
     8161 acagcacgac agcgcccttc cgcatgcgct tcttgcgggc agcacgaaat gcggacggaa
     8221 gcgcgggtgc actgacggtg ctcgccggta actgcgccac gccgggcggg ctgatcgacg
     8281 gtctcgacgc gacgaccgtg ccgcttcccg gtttcgcgaa ttttcccggt atcgggacga
     8341 tctcggtttt ggatcaggga cgcctgatct acgtagggtc gaacggcccc gtcgcgttca
     8401 agatcctcga cggtcgcatc tactcgtcgg atcttcccgt cggcagtgaa gggacgctcg
     8461 cgctcgatgc ggtgacgcgg acggtgctcg tcggccatgg cgccgtcgag gagcacatgg
     8521 cgggtgcacc cgggaccagc gatctgcaaa tccgcacgcg cctgaaggaa gacggcggca
     8581 cgcggtgcgc cgacgacaat gtcaacgcgg tcgacgcgtg cgtcgaggtc ggctatggaa
     8641 tccagagcac gcaagcgcgc gtctatttcg ccgacggcac cggcatcaac aaccaccgcg
     8701 cgtaccgcgt tcgcttcatc gatcgcgccg gccggctcca gacctacgtt ggaggtcacg
     8761 gcttctacgg cgaaggattg caccgccgca gcgtccgttc gcgcatgggt ggcatcgtgt
     8821 acaaaccggc aagtgcgagc aacggcgccg cgtttcctgc cggcctgtac ttcaccgatc
     8881 cgctcgatct cgtgatggcg cgaatcgatc cggcaaccga tcgagcctcg gtcgtgtggg
     8941 gcaatcgcag cggcgcgcca cgtacctaca acccgggcga gacgctcggg accgataagg
     9001 ggctcggctt caactacggt gccggaaacc tcgcggcgat gacgttcgac accgctggct
     9061 tgccgatgct tcgctatggc accgacgaag atcgcgctgg gctcctcacc gccgatgcga
     9121 tgcgacgagc gcagccattg atgaccggca cgcagcgctg ggagacgatg gccaacggcg
     9181 ccgatccaac aaccgcaggc ctgtggccgt acggtgcgct caacaacctc acgtactacg
     9241 cgggcaacct attcgtgctc ggcgggtaca cgaactacaa cgcgacctcg gtgctgaagc
     9301 gcttcgattt cgtgaacggc acgaccacgc gactcatggg gctcggcgcc gatgcgttca
     9361 ccccggatgg cgcaacaccg gcaaccgcga gcctctcggg cagctgccac gacggaatct
     9421 cgaagtgctt cacgcagtac aacgcggcgg acgatcgcct ctacttcagc gaggacaaca
     9481 ggctgcgcta cctcacgacg ccgcttggtc cgacgcagtc gctaggcacg ttgttcatcg
     9541 cgccaacgga cgtccgaaac ttcacgttcc gaccggacgg ttcgcaaatc tattacgcga
     9601 acggagggct ccgctgccac gacatcacga gtggctccgc ggcatgcaac gacaccgtgc
     9661 tcgggccacc cgcgttgctc ggcacgatcg gtctcgggcc cgatcagctc acgtggatcg
     9721 acaacaacac gctgctcatc agcacgcaca cgggccggat tctccagtac acggtgcaat
     9781 agcaagaggc gccaacgagt ccgcggggag aggaggagac cgcaggctcg ttggcgcctc
     9841 gaaacagagc atccgtaata acggatgagt tgtcaacggc cgtgtcgcgg ttctcgaaag
     9901 gccgcgcagc ggctcggaat ttcggtagcc atccggaggc actttcacgg cccaaaaggg
     9961 cagaatcgct gtgcgatgga cctcgagctg acggtggtgc agcgggccaa gccagagctc
    10021 ggctacatcg ctggcctggc cgtcggcgac gagctgatcg tggccgttgg cgggacgtcg
    10081 cggcatgcgc cgaccgtgtt ggcgtcgtcg gacgcgcgtc atttcgagcc gcgaaagact
    10141 ccgcggcagc tcggtctgcg cgatgtgctc gttgttggat cggcgatctg gacgtgtggt
    10201 gaatacggcc agctcgctgt ctcgcgcgat cgcggcgagt cgtggaaggt gatcgatacc
    10261 ggttgcgaca gctgtctgtt cggtctcgcg ctcgcggccg atggcgcggt gtgggtctgc
    10321 ggcgctgacg gctacgtcgc acgcatcgac gatccgcaga agcgactcga gcgcattcag
    10381 ctcgacacga cgacgcgcct caccgcgatc tattgcgtgc gcgatgaagt gatcgcgctc
    10441 gggctcgatg gcgtgatgta tcgctggcgc gcaggcgccg tgacgaagtt cgtgacgggc
    10501 gcaacgaagc agctcaccgc gctcgcgatc acgaaggcgg gcacgtggct cgtgatcggc
    10561 gatggcggat ttgtcgcgcg ctcgccggat ggcgcgtggt tctctcgcgt gaaagcgggc
    10621 gtcgagcacg atctggaatc gatcgcgccg ctcgcggatg gccgcgtcgt gatggtcggc
    10681 gaccgcggaa cgatcgtcct gtccaccgat gacggccgca cgtgggcgcc tgtggagagc
    10741 gacgtcagcg cgcatctatg gagtgcgcgg cgcttcggcg caggcctgct gatcggcggc
    10801 gacgacggcc tcgtgctcca gctcgcgccg caaggcgatc gcgcatggaa cgatcgcatc
    10861 gacatcttcg gcaacgtgaa gccgctcgat ggcgtgctcg cgctcggccc cgcggggttc
    10921 ctcggccgct tgccggcgta catcgcaacg ctcggttgcg aggcggcgaa agacgcgacc
    10981 gtcgcgccga gtacagatgc gttcgcgcgc atctacgggg tcgcgttgcc gtcagaagtc
    11041 gacgcgctat tcgcggcgac gcgcggacgt gactgcttcg acgagcttca cctcgacgcc
    11101 acactcatcg ccgacgttgg cgacgagaac gtgttcgagc gcgtgctcgc aagtggcgcc
    11161 accgacgcat tttgcggcgt cttctgcatc ggcgcgcaga gcagcggcgg ctcgtatcat
    11221 atggagctct acgagtggga cgggccgcgt caggtgctcc actacgatcg ctcgactcgc
    11281 gcattctccg gcgtgatcgc ggattcgctc gagagcctcg tctatctcgc agcgctcgtg
    11341 cgcgccgaga agcggcggct catctccgac gaggtcttca cgaacgggct gcgcaagctc
    11401 cacggcaagg tcgctccgac gttcgacgtc ggtgagggct tcgtgaagct cgacgcgaaa
    11461 cgtcgcgaca cggagttctt cttctatcgc tctcgctgga tctgcgcgct gctcgacggg
    11521 cgcgtggaaa tctcggagat cccgccgctg ttcatggcgg acttcaatca gattgttcca
    11581 aacgagcagc tgtccgcgcg gttcgaagcg tgcgaacgca tcatcccgac ggccctctac
    11641 tcgatgtggc gcgcgttcgt gttcgacgag cccgagctcg cgcgctatct cgagatcggg
    11701 agccgtcaca aatctcgcct cgttcgcgac gcggcaaagc tgatcgcaga gctgcgcaac
    11761 ggccgcaata cgctcggaac gatcggcgac gtccgcgcgc gtctcgctgc gttccgcgct
    11821 ctcgagctcg atccgcggcg cgcgatgcga cgcacagaga tcgaatccac acctcgcgat
    11881 cgttggacgg acctcgcgtg gcgctgggtc gacgatggcg tggcgcaacg cgcgctgctc
    11941 gcaaagctcg tcgaagaacc agcgctcgcc gcgcaggtcg aggcgctcga caagctgcgc
    12001 tggttgcccg acgtagaacg cgagcgcgca gtgcagcgcc tggcgaagca gctcgcaccc
    12061 gagctcgaag ccatcctcgc aggcagcatc gtgcgcggtg acgatctcag cgacgcgctc
    12121 ggccgtgtcg atcatgtcga cgagatcaag gcgcgcgccg tcgctgccca cgagcgcgtg
    12181 caagcagccc tcgcgatgac gacacgcgca ctcgcgctag atccgcacga cgacacgaca
    12241 gagtacacgc actccgtcct cctgctcgaa gcgggcaagg ccgaggagct ggtcgcgcgc
    12301 tggccgtcgc tcagctcgca gaaccaagag gcgctgatcg atcaaattgc gccggcgctc
    12361 aacgatgcat gcgttcaggc gcacgccgag aaagcattcg acgtcgccgt gcgactcgcg
    12421 caccacgcgc agcaattcgc gtcggcgcat ccatacctct atcactcggc cgcatgcgcg
    12481 ttcgcagcag tcggtgacta cgcgggtgca ttcgagcagg tgaagctcgc gatcgagcac
    12541 aacgccgaac gcgtcgcgga catggaggtc gattccgatc tcggcccgct gctcgactgg
    12601 cccgagttca aggc
//
LOCUS       DATLBZ010000575        14267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10075901, whole genome shotgun sequence.
ACCESSION   DATLBZ010000575 DATLBZ010000000
VERSION     DATLBZ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14267
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10075901"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1272)
                     /locus_tag="VMZ53_28505"
     CDS             complement(31..1272)
                     /locus_tag="VMZ53_28505"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS32489.1"
                     /translation="MRVGVIGCGTAGAAAAILLARVGCDVTVLERVAEPRPIGAGILL
                     QPTGQAVLARMGLLEPIRARGSHIDRLYLRTDGGRTLADLRYAAVDPTWFGIGLHRGL
                     LFETLFMAAKSEPNVRVQTGIDIRSVHWNVGQPTVSDVDGHEYGPFDLLVVSDGAVSE
                     LRTATGAAKRDTAYPWGALWFVTEDREQVFTKELYQIGVRAHRLYGVLPTGMGAVSSG
                     SDVPVVSLFWSLPSREVDAWRKSDLGAWKREVRALDPRMEFVLDRITSHEQVTFARYR
                     DVVMSRWCDRGVAFIGDAAHATSPQLGQGANLALVDAWTLADCVREEMARGRATGAAV
                     DMALTAYCRRRKGHLGYYQFMTRALTPFFQGDSRALGWLRDWIFPIANLLPPVRSFMT
                     KTMAGVSRGFVARPLELPPGS"
     gene            complement(1277..3556)
                     /locus_tag="VMZ53_28510"
     CDS             complement(1277..3556)
                     /locus_tag="VMZ53_28510"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS32490.1"
                     /translation="MQSETCFEIQDGAQSRVVALHTGPMTIGRAPTADVVIEHSWVAP
                     THARLESIAGAHHVIAVEGDVLLGGAPVKDAVLHDGDIVRLPDKTAANLVTLVYRNPL
                     APRIAPVQHFATPPGKALLSIGRAGTDIILDQPLVARRHAELLWQDNKHVLRDLGTVN
                     GTYVNGTRVQGSRALAPNDVVQIGTFRLTYDGDSLDSFDQRGAIRVDARNLKREVPGK
                     VLLDATTLSIEPCEFVAIVGASGAGKSTLMMALCGFERATGGTIHINGDDLYAGYDAY
                     RSIVGYVPQDDILHRSLTVTRALHHAARLRLPADTAESELEARIANVLDAVDMTEHRD
                     KRIDQLSGGQRKRVSIACELLNDPLLLFLDEPTSGLDPGLERKLMFTLRRLADGGRTV
                     VLITHATQNIRVCDHIIYLVEGKMVYFGPPAQALEFFGVEDFADIYAATDEPDKIEER
                     AETWQAKYRDSLQHQKYVIERPARAPSAPSVEQHAEAERRAKSAAQSRWRQLAILCRR
                     YMELLLSDRKNLALLLMQAPIIGLLLLFVSHHDGFVGNRIEAKKLVFMLALTGVWFGV
                     INSAREICKEVAVLRRERLGGLSQTSYLGSKLIVLAGLVTIQSALLVGTIALRIDMPA
                     HGILMPSLLEIYITVLLAGFAGITLGLALSAIASTPDKAMSLIPIVLVPQVLFAGVMF
                     MLVGATSVVGWLVSARPAVDALSAIVDINNVPSPIPVPFPFEPEHVHSVRMLLIPWTA
                     LLLQCLAFSSVAWWKIRKP"
     gene            3642..4037
                     /locus_tag="VMZ53_28515"
     CDS             3642..4037
                     /locus_tag="VMZ53_28515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32491.1"
                     /translation="MATKKQPSSRSKTKPAAAPQPKGRLHNLAAKPTSFGDEPTMDEQ
                     LVDNSGVFRVTAAIARDQIGHDEDAGDPRFASDSLVMAAQRDGFRERGPALDEVTEEV
                     AVDDEAAAVLKRERLRALAARDLARRQRR"
     gene            complement(4034..5473)
                     /locus_tag="VMZ53_28520"
     CDS             complement(4034..5473)
                     /locus_tag="VMZ53_28520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS32492.1"
                     /translation="MKLIFALAAVIVALPARANADVPAVSPYLYLNRCSGGCMVKGGF
                     DDARALTSSLPCNGTTTCGGGSCQCNGVGGGEFLIDEFEDTAGNVGAAADAEWKAILK
                     CVQEVYSPYNITVSDVPPPGGLSHSMAIVAGYPHNIGYRDTDIGGIAPGTLGCGPQDN
                     VVSFSFANTYAGPADHRILTLCYVASQESAHAFGVPDHTFNFIDGQSGCIDPMSYRGD
                     CGGQRFFRNKLANCGEGGPRPCKCPNQNSHKRLLSTFGPGTPITRPPAVRIDSPTMGT
                     VTNGQVVFATASAQRGIFRVELWLNGYKWASVPGAKWGANGQPESQYALTFPAMVPDG
                     VIDIVVKAFDDIEAKAETSVITVTKGQPCMSADSCLKGQKCDAGKCFWEEPRGQVGDA
                     CDYPQFCISSDCLNTDQGQYCSQDCVVGVSDSCPAGFSCEGAAGATGKCVKEDVGGGC
                     CSVGDDGKTAALFTLIVGGLLGRRRRVKR"
     gene            complement(5470..5814)
                     /locus_tag="VMZ53_28525"
     CDS             complement(5470..5814)
                     /locus_tag="VMZ53_28525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32493.1"
                     /translation="MLRGVCLLMLVCALAGRANADTTSTTSVSQLMVLEPGDSSYKLF
                     HGAVWLDVDKATTNYRWGGLQCKGRDLSDTSVQVLFAAFRSEYTVRLEYVITDYKEKQ
                     YRCVTGFTVSKS"
     gene            complement(5828..7276)
                     /locus_tag="VMZ53_28530"
     CDS             complement(5828..7276)
                     /locus_tag="VMZ53_28530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32494.1"
                     /translation="MPRAGWLFVLVVVARGASAEPRRVAVDTPLEAFTPAATGTATYL
                     YLNRCTGGCQIQGTGGSGVGVNDASANMTTIAASGTYTVGEYRDAMNQTGALADAEWA
                     QLVKCMREVYSPYAVEVSDVRPSDGRAFNMAVIAGRPQEVGLGNDILGIAPLANDCSP
                     QSNVISFSFANYHGSFGRVLNVCWTAAQETSHAYGLDHEYQFTTGESACSDPTTYRTD
                     CGGQKFFRNRRAQCGEDGVRKCRCGSSQNSHAKLITVFGEGTPITAAPHVSLGFPTNG
                     STVGSSWVVHALAGSARGIAKVELYLNGSRWHVVPGQGFGANGQADPSDYTFMVSTKV
                     PDGLIDVQAKAYDDLGIASDSEIVTVTKGAACTMDAQCLADQHCQAGHCTWPAPEGEL
                     GASCEYDQQCKGWTCLGDEDSKYCTVPCYTDEPTSCPSDFTCAPTGNQTDGFCLPEGG
                     GCCSASGDGGRGLWATACLSVLVLTWLRRRRR"
     gene            7302..7712
                     /locus_tag="VMZ53_28535"
     CDS             7302..7712
                     /locus_tag="VMZ53_28535"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HUS32495.1"
                     /translation="MAWTCPDCKRSFGRRNQSHECAPSNTVDDYFAKRPVELRKIYNA
                     IERVVTKLGGHIDPVKVCVMFKRARTFGEVRAMKDRLRLYFILSREILDDRIDKRLRM
                     SAHRCVHQIDLTSPKQVDRDVRDWLAEAYACSPL"
     gene            complement(7769..9952)
                     /gene="fusA"
                     /locus_tag="VMZ53_28540"
     CDS             complement(7769..9952)
                     /gene="fusA"
                     /locus_tag="VMZ53_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HUS32496.1"
                     /translation="MLTDLSKVRNIGISAHIDSGKTTLTERILYYTARIHAIHEVKGK
                     DGVGAKMDSMELERERGITIQSAATFCQWPPDAKAQKTVQAVPNSPIKAQHHINIIDT
                     PGHVDFTVEVERSLRVLDGAIMVLCGVAGVQSQSYTVDRQMRRYKVPRIAFVNKLDRT
                     GANPFRVRDQLREKLKLNPVMIQCPIGLEDKLQGVVDLIEMDAYTFHGENGEQIERGP
                     IPADAMHPLASHGHSGTLKELCEKMREEMLDGLSLVNDELAEAILEDKVTPELIRKCL
                     RDSTVALKCTPVMCGSALGNKAVQLLLDGVCSYLPEPREVENIALDLDKEETPVVIPS
                     DPTKPLVMLAFKLEDGRYGQLTYIRVYSGAVRKDDFIVNSRNGKKVKVGRLVRMHSDD
                     KEDITEAGAGDICALFGVDCNSGDTFTDGSVNYAMTSMFVPSPVISVAIKAKDSGAEI
                     NMSKALNRFTKEDPTFKCWVDPESNETIVAGMGELHLDVYIERMKREYKAEVVTSPPQ
                     VAYRETIGAKVDFNYTHKKQTGGSGQYGRVAGYVEPFDGEFEFVDEIKGGSIPREFIP
                     SCEKGFKSMIPKSPRLGVPCVGIRVVINDGQSHAVDSSDNAFQEAARGAFREFFPRAK
                     PKILEPIMKVSLEGPAEFSGNMLGLIMQRRGIVVGSTEEDGNARVDAEVPLSEMFGFS
                     TPLRSSTQGKAEFTMEFAKYSEVPNNVGEELLAKAKKAKEEASKK"
     gene            10091..11890
                     /locus_tag="VMZ53_28545"
     CDS             10091..11890
                     /locus_tag="VMZ53_28545"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32497.1"
                     /translation="MECLDDNAASEFVSGSLAATAVTQVERHLASCRDCRALVAALAG
                     DEADSNALTYKHEKIEDSQVVKRPTLSIGDRVGRYLVLSSIGAGGMGVVFAAYDPQLD
                     RKVALKLLRANLAGVNAKEARTRLKREAQAIAQLNHPNVVGVYDVGTTDDGDVYIAME
                     FVEGDTLTTWLKKWPRTWREILEVFHQAARGLMAAHSVGLLHRDFKPDNVLVGGDGRV
                     RVTDFGLARSLFLDTDSARGAVTGVAQGSGPKPAHGSPLGVDLTATGTVLGTPRYMPP
                     EQLMGPTIDARADQFSFCVALYEALWSKHPLKGATSVAMLEEDAKAQPPPESKVPSSI
                     VRAVMRGLEKDRAKRFPTLSALIHELTPPQTRSPAKFIMFAAVGALFVTGATAAVMTT
                     DTPTVRKDGGDEETVRLLISQINQRDQEIKRLRDVITKGVKDREELVRIKQELDDAQQ
                     EVQALTDQVIELKAKQVPNGKPVVAQITSGKITDAIEMERKSLEFCLSDIDSRVLSTE
                     GAPKTRDIDALVRLTISPKGTVIDETVKTSGIEYTPATKFCIEAALKRVAYPQGADFL
                     DTRVTIAWKAGVLTMVANVVGQRAPSPSDLTGI"
     gene            12001..13527
                     /locus_tag="VMZ53_28550"
     CDS             12001..13527
                     /locus_tag="VMZ53_28550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS32498.1"
                     /translation="MSRLLLSSCLALVAMSATGSAENEDHGLPQTDGATKTWKGYPVY
                     GTTHQYVALPDPPKAIARTAAEPQISPYIYLNRCTGGCLIKGAAEGQRDDARQNISFI
                     ATPGDHLVSEFKNKAGQTGAAADAEWNQIVKCMKEVYSPFGVMLTDQKPVGVSYTMAV
                     IAGTPQEIEYDPMAAGVAPAGCDPQDNVISFSFANYDNAADRVNSICWTAAQETAHAF
                     GLDHEYQFSDGTSACNDPMTYRYDCGGQKFFRNKAAQCGENSARTCRCGGSQNSHIKI
                     RSVFGDGTSLIPAPTCVVTNPTSGSVNNGAVITATSGSQRGVARVELWLNNYKWTDVP
                     GAKFGLTGQANPSTYSLTFPGNVPDGKIDMVVKCFDDLGLEKDSQMITVQKGNCNDAA
                     SCKCLAGQQFADGKCFWEAPVGELGDDCTFPQYCKTGICTETDQGAFCSSDCIIGSSD
                     GCPMSFECIQTGAATGQCLPVSEGGCCSASTGANQVWAQMGLGGLILGFIVRRRRRRR
                     "
     gene            complement(13531..>14267)
                     /locus_tag="VMZ53_28555"
     CDS             complement(13531..>14267)
                     /locus_tag="VMZ53_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32499.1"
                     /translation="FAREVERRAPSIATAKWWKEERQGVFLDYNQNARDRTTCCAYSV
                     RAREDARVSMPLSWDDFVVANPADFTLRTVPAIFKARGDAHAAIDDHPGDITKLLELA
                     DRQQAEGAADAPWPPHFAKGEGEAPRVAPSRARKPGAASRKPKLPVITIAQAKLKPDA
                     LAGLERWKARHPGVVTHLAPEDVLVDTNRGRATAWYRIRINLKNVPPEQHPPPEEPDP
                     NYDPRSEYAGMTWDGKPNADGAGEDE"
BASE COUNT         2500 a         4697 c         4659 g         2411 t
ORIGIN      
        1 cgcctcgcga tcgcccgcgc gttcgcggtg ttacgagccg ggcggcaact cgagtggtcg
       61 ggccacgaag cccctggaga ctcctgccat cgtcttcgtc atgaagctcc gcacgggcgg
      121 caggaggttc gcgatcggaa agatccaatc gcgcagccac ccgagcgcgc gcgagtcacc
      181 ctgaaagaac ggcgtcagcg cgcgggtcat gaactggtag tagccgaggt gcccctttcg
      241 ccgccggcaa tacgcagtga gtgccatatc gaccgccgcg ccggtcgcgc ggccgcgcgc
      301 catctcctcg cgcacgcaat cggccagcgt ccaggcatcc acgagcgcca ggttcgcgcc
      361 ctgtcccaac tgcgggctgg tcgcgtgcgc cgcatcgccg atgaaagcaa caccccgatc
      421 acaccatcgg ctcatcacga cgtcgcgata ccgcgcaaac gtcacctgct cgtgcgacgt
      481 gatccgatcg agcacgaact ccatgcgcgg atcgagcgcc cgaacctcgc gcttccacgc
      541 gccgagatcg ctcttgcgcc acgcatcgac ctcgcgcgac ggcagcgacc agaagagcga
      601 cacgaccggg acgtcactac cgctcgagac cgcccccatc ccagtcggca acacgccata
      661 cagccggtgc gcgcgtacgc cgatctgata gagctccttc gtaaacacct gctcgcggtc
      721 ctcggtcacg aaccacagcg cgccccacgg atacgccgtg tcgcgtttgg ccgcgcccgt
      781 tgcggtccgt agctcggaca cagcgccatc cgagacaaca agcagatcga acggtccgta
      841 ttcgtggccg tccacatcac taacagttgg ttgaccgaca ttccaatgga ccgaacgaat
      901 gtcgatgccg gtttgaaccc gtacattcgg ttcgctcttc gctgccataa acagcgtctc
      961 gaacaggagg ccgcggtgca ggccgatgcc gaaccacgtc gggtctaccg ccgcgtagcg
     1021 gagatcagcg agtgtccggc cgccgtccgt gcgcaagtag aggcgatcga tatgcgagcc
     1081 gcgtgcgcga atcggctcga ggaggcccat gcgcgcgagc acggcctgcc cggtcggctg
     1141 cagcaggatg ccagcgccga tgggccgagg ctcggcgacc cgctcgagca ccgtgacgtc
     1201 gcagcccacg cgcgccagca gaatcgccgc cgccgcgccc gccgtaccgc acccgatcac
     1261 gccgacgcgc atcacgtcac ggtttgcgaa tcttccacca cgcgaccgac gagaaggcga
     1321 ggcactgcag gagcagcgcg gtccacggga tcagcagcat gcggacgctg tgcacgtgct
     1381 cgggctcgaa cgggaacggg acggggatcg gagagggcac gttgttgatg tcgacgatcg
     1441 cggacagcgc atcgaccgcg gggcgcgcgg acaccagcca accaacgacc gaggtcgcgc
     1501 ccacgagcat gaacatcacg ccggcgaaca ggacttgcgg gacgagcacg atcgggatga
     1561 gcgacatcgc cttgtccggc gtcgacgcga tcgcggagag cgcgaggccg agcgtgatgc
     1621 cggcaaagcc tgcgagcagg acggtgatgt agatctcgag cagcgatggc atcaggatgc
     1681 cgtgcgcggg catgtcgatg cgcagcgcga tcgtgccgac gaggagcgcg ctctggatcg
     1741 tgacgagccc cgcgagcacg atgagcttgc tgccgagata ggacgtctgc gagaggccgc
     1801 ctaatcgctc gcggcggagg accgcgacct ctttgcagat ctcgcgcgcc gagttgatca
     1861 cgccgaacca cacgcccgtg agcgcgagca tgaagaccag cttcttcgct tcgatgcggt
     1921 tcccgacgaa accgtcgtgg tgcgaaacga acagaaggag gagcccgatg atcggcgcct
     1981 gcatcagcag cagcgcgagg ttcttgcgat cggacagcag cagctccatg tagcgcctgc
     2041 acaggatcgc gagctggcgc cagcgcgatt gcgcggcgga cttcgcgcgg cgctcggctt
     2101 cggcgtgctg ctcgaccgat ggagcggagg gcgcgcgggc agggcgctcg atcacgtact
     2161 tttggtgctg gagcgagtcg cgatacttcg cctgccacgt ctcggcccgt tcttcgatct
     2221 tgtcgggctc gtcggtcgcg gcatagatgt cggcgaagtc ttcgacgccg aagaactcga
     2281 gcgcctgcgc cggcggcccg aagtagacca tcttgccttc gaccagatag atgatgtggt
     2341 cgcacacgcg gatgttctgc gtcgcgtgcg tgatgagcac gaccgtgcgg ccaccgtcgg
     2401 ccaggcggcg cagcgtgaac atcagcttgc gctcgaggcc cgggtccagg ccactcgtcg
     2461 gctcgtcgag gaagaggagg agagggtcgt tgagcagctc gcaggcgatc gacacgcgct
     2521 tgcgctggcc gcccgagagc tggtcgatcc gcttgtcgcg atgctcggtc atgtcgaccg
     2581 cgtcgaggac gttcgcgatg cgcgcttcga gctcggactc tgcggtgtcg gcggggaggc
     2641 gcaggcgtgc ggcgtggtgc agcgcgcgcg tcacggtgag cgagcgatgc aggatgtcgt
     2701 cttgcggcac gtagccgacg atcgagcggt acgcgtcgta accggcgtag agatcgtcgc
     2761 cgttgatgtg gatcgtgccg cccgtcgcgc gctcgaagcc gcacagcgcc atcatcagcg
     2821 tcgatttgcc agcgccgctc gcgcccacga tcgcgacgaa ctcgcagggc tcgatcgaga
     2881 gcgtcgtcgc gtcgagcagg actttgccgg gaacctcgcg cttgaggttg cgcgcgtcga
     2941 cgcgaatcgc gccgcgttga tcgaacgaat cgaggctgtc gccgtcgtac gtgaggcgaa
     3001 acgtgccgat ctgcacgacg tcgttcggcg cgagcgcgcg cgagccttgg acccgcgtgc
     3061 cgttgacgta cgtgccgttc accgtgccga ggtcgcgcag cacgtgcttg ttgtcttgcc
     3121 agagcagctc tgcgtggcgg cgcgcgacga gcggctggtc gaggatgatg tcggtgccgg
     3181 cgcgcccgat cgagagcagc gccttgccgg gcggcgtcgc gaagtgctgt accggcgcga
     3241 tgcggggcgc gagcgggttg cggtacacga gcgtgacgag attcgccgcc gtcttgtccg
     3301 gaagccggac gatatcgcca tcgtggagga ccgcatcttt caccggtgcg ccgccgagca
     3361 ggacatcgcc ttcgaccgcg atcacgtggt gagccccggc gatcgactcg aggcgcgcat
     3421 gcgtcggggc tacccacgaa tgctcgatca cgacgtcagc ggtcggcgct cgcccgatcg
     3481 tcatcggtcc cgtatggagc gcgacgacgc gcgactgcgc accatcttga atctcgaagc
     3541 aagtctccga ctgcaaagta tgacccgcag gataacaccc acaattttcg tccgcagcga
     3601 accggccggc tcgtgtacga tgggtagcta gggggaacgt catggctacg aagaagcagc
     3661 cgtcttcgcg ctccaagacc aagccagcag ccgcgccgca gccgaagggc cgcctgcaca
     3721 atctcgcggc gaagccgaca tcgtttggcg acgagccaac gatggacgag cagctcgtcg
     3781 acaacagcgg tgtgttccgc gtaaccgctg cgatcgcgcg cgatcaaatc ggccacgacg
     3841 aggacgccgg cgatccgcgg ttcgcgagcg acagcctcgt gatggccgcg caacgcgatg
     3901 gcttccgcga gcgagggcct gcgctcgacg aggtcaccga ggaagtggcc gtcgacgacg
     3961 aagcggccgc cgtgctcaag cgcgaacgtc tccgtgcgct cgccgctcgc gacctcgctc
     4021 gccgtcagcg tcgctagcgc ttcacgcgtc gccggcgtcc gagcaagccg cccacgatca
     4081 acgtgaagag cgcggctgtc ttgccgtcat caccgacgct gcagcatccg ccgccgacgt
     4141 cttctttgac gcacttgcca gtcgcaccgg ctgcgccttc gcacgagaac cccgccgggc
     4201 agctgtccga aacgccgacg acacagtcct gcgagcagta ctggccttgg tcggtattga
     4261 ggcagtcgct gctgatgcag aactgcggat agtcacacgc gtcgccgact tgcccgcgcg
     4321 gctcctccca gaagcacttc ccggcgtcgc acttctggcc cttgaggcag gagtccgcgg
     4381 acatgcacgg ttggcccttc gtcaccgtga tgaccgacgt ctccgccttt gcctcgatgt
     4441 cgtcgaacgc tttgacgacg atgtcgatca caccgtcggg caccatcgcc gggaatgtca
     4501 gcgcgtactg cgattccggc tggccgttcg cgccccactt cgcgcccggc acgctcgccc
     4561 acttataacc gttgagccac agctcgacgc ggaagatgcc gcgctgtgca gacgccgttg
     4621 cgaacacgac ctggccgttc gtgaccgtgc ccatcgtcgg cgaatcaatg cgcaccgccg
     4681 gcggccgcgt gatcggcgtg ccgggaccga acgtcgagag cagacgcttg tgcgagttct
     4741 ggttcgggca tttgcacggg cgcggtccac cctcgccgca gttcgcgagc ttgttgcgga
     4801 agaaccgctg cccgccgcag tcgccgcgat aggacatcgg atcgatgcag ccggactgac
     4861 catcgatgaa gttgaacgtg tgatcgggaa cgccgaacgc gtgcgccgac tcctgggacg
     4921 cgacgtagca gagcgtgagg atccggtgat cggcgggacc cgcgtacgtg ttcgcaaacg
     4981 aaaacgagac cacgttgtcc tgcggaccac agccgagcgt gccgggcgcg ataccgccga
     5041 tatccgtgtc gcgatagccg atgttgtgcg gataccccgc gacgatcgcc atcgagtgcg
     5101 acagaccgcc gggcggaggc acgtcgctca ccgtgatgtt gtacggcgag tacacctcct
     5161 gcacgcactt gaggatcgcc ttccactcgg cgtccgctgc ggcaccgacg ttacccgcgg
     5221 tgtcctcgaa ctcgtcgatc aagaactcgc cgccgccaac gccattgcac tgacagctgc
     5281 cgccaccaca cgtcgtcgtg ccgttgcacg gaagcgagga cgtcaacgcg cgcgcgtcgt
     5341 cgaagccgcc cttcaccatg cagccaccgc tgcagcggtt cagatacaga tatggcgaga
     5401 cggccgggac atcagcgttc gcgcgcgccg gcaatgcgac gatcacagcg gcgagcgcga
     5461 agatcagctt catgacttcg agaccgtgaa cccggtgacg cagcggtact gcttctcttt
     5521 gtagtccgtg atcacgtact cgagccgcac cgtgtactcg gagcgaaacg cagcaaacag
     5581 cacctgcacg ctcgtatcgc tcagatcgcg acctttgcac tgcaggcctc cccagcggta
     5641 gttcgttgtc gccttgtcga cgtcgagcca cacggcgccg tgaaacagct tgtagctcga
     5701 gtcaccgggc tcgaggacca tcagctgcga cacggacgtc gtcgacgtcg tatcggcatt
     5761 cgcgcgccca gcgagcgcac agacgagcat cagcagacac acaccacgca acatgcgcag
     5821 gagcatacta cctccggcgt cgacgcagcc acgtgagcac gagcacgctc aaacatgccg
     5881 ttgcccatag cccgcgcccg ccatcgccgg aggcgctgca gcagccgccg ccctcgggca
     5941 ggcagaaccc atcggtctga tttcccgtcg gtgcgcacgt gaagtcggac ggacagctcg
     6001 tgggctcgtc ggtgtagcag ggcaccgtgc agtacttgct gtcctcgtcg ccaaggcacg
     6061 tccagccctt gcactgctgg tcgtactcgc agctggcgcc gagctcgccc tcgggtgcgg
     6121 gccacgtgca gtgcccagct tggcaatgct ggtcggcgag gcactgcgcg tccatcgtgc
     6181 acgcggcgcc cttcgtcacg gtcacgatct cgctgtcgct cgcgatgccg aggtcgtcgt
     6241 acgcctttgc ctgcacgtcg atcaggccat cgggcacctt cgtcgacacc atgaacgtgt
     6301 agtccgacgg atcggcttgt ccgttcgcgc caaagccctg gccaggcacc acgtgccagc
     6361 gactgccgtt gaggtagagc tcgaccttcg cgatgccgcg cgcagaaccg gcgagcgcat
     6421 gcacgaccca gctcgagccg accgtgctgc cgttcgtcgg aaagccgagc gagacgtgcg
     6481 gcgccgccgt gatcggcgtg ccttcgccga acaccgtgat gagcttcgcg tgcgagttct
     6541 gcgaggagcc acagcggcac ttgcgaacgc cgtcctcccc gcactgcgcg cggcgattgc
     6601 ggaagaactt ctgcccgccg caatccgtcc gatacgtcgt cggatcgctg cacgccgatt
     6661 caccggtcgt gaactgatac tcgtggtcga ggccgtacgc gtgcgacgtc tcttgcgcgg
     6721 cggtccagca cacgttgagc acgcgcccga acgagccgtg atagttcgca aacgaaaacg
     6781 agatcacgtt cgactgcggg ctgcagtcat tcgcgagcgg cgcgatgccg aggatgtcgt
     6841 tgccgagccc gacttcctgc ggccgtcccg caatcaccgc catgttgaac gcgcgcccat
     6901 cgctcggccg cacgtccgaa acctcgacgg catacggcga atacacctcg cgcatgcact
     6961 tcacgagctg cgcccactcg gcatcggcga gcgcgccggt ctggttcatc gcatcgcggt
     7021 actcgccgac ggtgtacgtg ccgctcgcgg cgatcgtcgt catgttcgcc gacgcatcgt
     7081 tgacgccgac gcccgagccg cccgtgccct gaatctgaca gccgccggtg cagcgattca
     7141 aatacaagta cgtcgccgtc ccggtcgctg cgggcgtgaa cgcttcgagc ggtgtatcga
     7201 ccgctacgcg ccgcggctcg gcactcgccc cacgggcgac gaccacgagc acgaacaacc
     7261 acccggcacg tggcattcga tcagatgata gccgaagcgc gatggcctgg acatgcccgg
     7321 actgcaagcg ttcgttcggc cggagaaacc agtctcacga atgtgcgcca agtaacaccg
     7381 tcgacgacta cttcgcgaag cggcccgtcg agctccgcaa gatctacaac gcgatcgagc
     7441 gcgtcgttac gaagctcggg ggccacatcg accccgtgaa ggtctgcgtc atgttcaagc
     7501 gcgctcgaac gttcggcgaa gtgcgcgcga tgaaggaccg gctgcgcctc tacttcatcc
     7561 tctcgcgcga gatcctcgac gaccgcatcg acaaacggct gcggatgtcg gcgcaccgct
     7621 gcgtgcacca aatcgatctg acatcgccca aacaagtgga ccgcgatgtc cgcgattggc
     7681 tcgctgaagc gtacgcctgc agcccgctgt agccgaaaac gaaaacgccg acccgaaggc
     7741 cggcgtcgca gcactgcgcg agcagtgact acttcttcga agcctcttcc ttggccttct
     7801 tggccttggc gaggagctct tcgccgacgt tgttcggcac ttcggagtac ttcgcgaact
     7861 ccatcgtgaa ctcggccttg ccctgcgtcg agctgcggag cggcgtcgag aagccgaaca
     7921 tttccgagag cggaacctcg gcgtcgacgc gcgcgttgcc atcttcttcg gtcgaaccga
     7981 cgacgatgcc gcggcgctgc atgatgaggc cgagcatgtt gcccgagaac tccgccggac
     8041 cctcgagcga gaccttcatg atgggctcga gaattttcgg cttcgcgcgc gggaagaact
     8101 cgcggaatgc accgcgggct gcttcctgga atgcgttatc gctcgagtcg actgcgtgcg
     8161 actgaccgtc gttgatgacg acgcggatac cgacgcacgg cacgccgagg cgtgggctct
     8221 tcgggatcat cgacttgaag cccttttcac acgacgggat gaactcgcgc ggaatcgagc
     8281 cgcccttgat ctcgtcgacg aactcgaact cgccgtcgaa cggctcgacg taaccagcga
     8341 cgcggccgta ctgaccggag ccgcccgtct gcttcttgtg cgtgtagttg aagtcgacct
     8401 tcgcgccgat cgtctcgcgg tacgcgacct gcggcggcga ggtgacgacc tcggccttgt
     8461 actcgcgctt catgcgctcg atgtagacgt cgaggtggag ctcacccatg ccggcgacga
     8521 tcgtctcgtt cgactcggga tcgacccagc acttgaacgt cgggtcttcc ttcgtgaagc
     8581 ggttgagcgc cttcgacatg ttgatctcgg cgccggagtc cttcgccttg atggcgacgg
     8641 agatgaccgg cgacgggacg aacatcgacg tcatcgcgta gttgacgctg ccgtcggtga
     8701 acgtgtcgcc cgagttgcaa tcgacgccga acagcgcgca gatgtcgcct gcgccggcct
     8761 cggtgatgtc ttccttgtcg tccgagtgca tgcggacgag gcggccgact ttgaccttct
     8821 tgccgttgcg cgagttgacg atgaagtcgt ccttgcgcac ggcgcccgag tacacgcgga
     8881 tgtacgtgag ctggccgtag cgaccatcct cgagcttgaa cgcgagcatg acgagcggct
     8941 tcgtcggatc cgacgggatg acgaccggcg tctcttcctt gtcgagatcg agggcgatgt
     9001 tctcgacctc acgcggctcg gggagatacg agcaaacgcc gtcgagcagc agctggacgg
     9061 ccttgttgcc gagcgccgag ccgcacatga cgggcgtgca cttcagtgca accgtcgagt
     9121 cgcgcaggca cttgcggatc agctccgggg tgaccttgtc ttcgaggatc gcttcggcga
     9181 gctcgtcgtt gacgagcgag agaccgtcga gcatctcttc gcgcatcttc tcgcagagct
     9241 ctttgagcgt gccgctgtgg ccatgcgacg cgagcgggtg catcgcgtcg gccgggatcg
     9301 ggccgcgctc gatctgctcg ccgttctcgc cgtggaacgt gtacgcgtcc atctcgatca
     9361 gatcgacgac gccctggagc ttgtcctcga ggccgatcgg acactggatc atgaccgggt
     9421 tgagcttcag cttctcgcgg agctgatcgc ggacgcggaa cgggttcgcg ccggtgcggt
     9481 cgagcttgtt gacgaacgcg atgcgcggaa ccttgtagcg gcgcatctgg cgatcgaccg
     9541 tgtacgactg cgactgcacg ccggcgacgc cgcagagcac catgatcgcg ccgtcgagca
     9601 cgcggagcga gcgctcgact tcaaccgtga agtcgacgtg tccgggggtg tcgatgatgt
     9661 tgatgtggtg ctgcgccttg atcggcgagt tcggaacggc ttggaccgtc ttctgcgcct
     9721 tggcgtcggg tggccactgg cagaacgtcg cggccgattg gatcgtgatg ccgcgctcgc
     9781 gctccagctc catcgaatcc atcttggcgc cgacgccgtc cttgcccttc acctcgtgaa
     9841 tcgcgtggat gcgggcggtg taatagagga tgcgttcggt cagcgtcgtc ttgcccgagt
     9901 cgatgtgggc cgagatgccg atgttgcgga ctttggacag gtcggtcagc ataaaagcgc
     9961 gtgattgcta ccgtgtaact tgctctaatg caagcgaaac cccgccgctc gggcagtgtc
    10021 caagtagctg aggcacagca cgatgcattt caagcggttg gcagaaccgg tgcgctaccc
    10081 tgaatggaca gtggagtgtc tcgacgacaa tgcggccagc gaatttgtct cgggctctct
    10141 cgcagccacc gcggtcacac aggtcgagcg acacctcgct agctgccgcg actgccgcgc
    10201 tctcgtagct gcgctcgccg gtgacgaggc cgactccaac gcgctgacgt acaagcacga
    10261 gaagatcgag gactcgcagg tcgtcaaacg gccgacgctc tcgatcggtg atcgcgtggg
    10321 tcgctacctc gtgctgtcgt cgatcggcgc gggcggcatg ggcgtcgtgt tcgcagcgta
    10381 cgatccgcag ctcgatcgca aggtcgcgct caagctcctc cgcgcaaacc tcgcgggcgt
    10441 caacgcgaag gaagcccgca cgcgtctcaa gcgtgaggcg caggcgattg cgcagctcaa
    10501 tcatccgaac gtcgtcggcg tctatgacgt cggcaccacc gatgacggcg acgtgtacat
    10561 cgcgatggag tttgtcgaag gcgacacgct gacgacgtgg ctcaagaagt ggccgcgcac
    10621 gtggcgcgag atcctcgagg tctttcatca ggccgcgcgc ggtctcatgg ccgctcactc
    10681 ggtcggcttg ctccaccgcg acttcaagcc cgacaacgtc ctcgtcggtg gcgatggtcg
    10741 cgtgcgcgtg acggactttg gcctcgcacg ctcgctcttc ctcgacaccg acagcgcgcg
    10801 tggtgcggtc accggcgtgg cgcaaggctc cggtcccaag cctgcgcatg gcagcccgct
    10861 cggcgtcgat ctcaccgcga cgggcacggt gctcggcaca ccgcgctaca tgccgccgga
    10921 gcagctgatg gggccgacga tcgacgcgcg cgctgatcag ttcagcttct gcgtcgcgct
    10981 ctacgaagcg ctgtggagca agcatccgct caaaggtgcg acgagcgttg cgatgctcga
    11041 ggaggacgcg aaggcgcagc cgcctcccga gagcaaggtt ccgtcgtcga tcgtgcgcgc
    11101 ggtcatgcgc ggtctcgaga aggatcgcgc gaagcgattc ccgacgctgt cggcgctcat
    11161 ccacgagctg actccaccgc agacccgctc gcccgcaaag ttcatcatgt tcgccgcggt
    11221 cggcgcgctg ttcgtgacgg gggcgaccgc agcggtgatg acgaccgaca cgccgacggt
    11281 gcgcaaagac ggcggcgatg aagagacggt gcgtctgctc atctcgcaga tcaatcagcg
    11341 cgatcaggag atcaaacgcc tgcgcgacgt gattacgaaa ggcgtcaaag atcgcgaaga
    11401 gctcgtgcgc atcaagcagg agctcgacga tgcgcaacaa gaagtccaag cgctgaccga
    11461 tcaggtcatc gagctcaagg ccaagcaggt tccgaacggc aagccggtcg tcgcgcagat
    11521 caccagcggc aagatcaccg atgcgatcga gatggagcgc aagagcctcg agttctgcct
    11581 cagcgacatc gacagccgcg tcctctcgac cgagggcgca ccgaagaccc gcgacatcga
    11641 cgcgctagtt cgcctgacga tctcgcccaa aggcaccgtc atcgacgaga cggtgaagac
    11701 gtcagggatc gagtacaccc cggccacgaa gttctgcatc gaggccgcgc tcaagcgcgt
    11761 ggcgtacccg caaggtgcgg actttctcga tacgcgcgtc acgatcgcgt ggaaggccgg
    11821 cgtcctgacg atggtcgcca atgtcgtggg ccagcgcgcg ccttctccga gcgatctcac
    11881 aggcatttag ccgcacctga tgcgtctggt ggattgcctg aaaggtgcgg tgagccgcaa
    11941 cctccggaca tgtgacgaaa cctgctgacg cggcgcgcgg ttgtgtagta tccgctcccc
    12001 atgtcccgcc tgcttctgtc gagctgtctt gcactcgtcg cgatgtcggc caccggctcc
    12061 gcggaaaacg aagaccatgg tctgccgcag accgacggcg ccacgaagac gtggaagggt
    12121 tatcccgtct acggcacgac gcaccagtac gtagcgctgc cggatcctcc gaaggcgatc
    12181 gcgcgcacgg cggccgagcc gcaaatctcg ccgtacatct atctcaaccg ctgcacgggc
    12241 ggctgcctca tcaagggcgc ggccgaaggt caacgcgacg acgcacgcca gaacatctcg
    12301 ttcatcgcga cgccgggcga ccacctcgtc agcgagttca agaacaaggc aggacagact
    12361 ggcgccgccg ccgacgcgga gtggaaccag atcgtcaagt gcatgaaaga ggtctactcg
    12421 cctttcggcg tgatgctcac ggaccagaag cccgttggcg tctcgtacac gatggccgtg
    12481 atcgccggta cgccgcagga gatcgagtac gacccgatgg ccgctggtgt cgcgcccgct
    12541 ggctgtgacc cgcaggacaa cgtcatctcg ttctcgttcg cgaactatga caacgcggcc
    12601 gatcgcgtga acagcatctg ctggaccgca gcgcaggaga ccgcgcacgc gttcggtctc
    12661 gatcacgagt atcagttcag cgatggcacg tcggcgtgta acgacccgat gacgtatcgc
    12721 tacgactgcg gcgggcagaa attcttccgc aacaaggcgg cgcagtgcgg cgagaactcg
    12781 gcgcgcacgt gccgctgcgg cggatcgcag aactcgcaca tcaagattcg ctcggtgttc
    12841 ggtgacggca cgtcgctcat tcctgctccg acgtgcgttg tgacgaaccc gacgagtggc
    12901 tccgtcaaca acggcgccgt catcaccgcg acgagtggct cgcagcgtgg cgtcgcgcgg
    12961 gtcgagctgt ggctcaacaa ctacaagtgg acggacgtgc cgggcgcgaa gttcgggctc
    13021 accggccaag cgaacccgtc gacgtactcg ctgacgttcc cgggcaacgt tcccgacggc
    13081 aagatcgaca tggtcgtgaa gtgcttcgac gatctcggcc tcgagaaaga ctcccagatg
    13141 atcacggtcc agaagggcaa ctgcaacgac gccgcgagct gcaagtgcct cgcgggtcag
    13201 cagttcgccg acggcaagtg cttctgggaa gcgccggtcg gcgagctcgg cgatgactgc
    13261 acgttcccgc agtactgcaa gacgggcatc tgcacggaga ccgaccaggg cgccttctgc
    13321 tcgtcggact gcatcatcgg ctcgagcgat ggttgcccga tgagcttcga gtgcatccag
    13381 accggcgcgg cgacgggcca gtgtctcccg gtgagcgaag gcggatgctg cagcgcttcg
    13441 acgggcgcga atcaggtgtg ggcacagatg ggcctcggtg gcctcatcct cggcttcatc
    13501 gttcgccgtc gccgtcgtcg ccgctagtcg tcactcgtct tcgccggcgc cgtcggcgtt
    13561 cggcttgccg tcccacgtca tgcccgcgta ttcgctgcgc gggtcgtagt tgggatcggg
    13621 ctcctcgggc ggcggatgct gctcgggcgg gacgttcttg aggttgatgc ggatgcggta
    13681 ccacgcggtc gcacggcctc ggttcgtgtc gacgaggacg tcctcgggcg cgagatgcgt
    13741 gacgacgccg ggatggcgcg ccttccaccg ctcgaggccg gcgagcgcat cgggcttgag
    13801 cttggcttgc gcgatcgtga tgacgggcag cttcggcttg cggctggctg cacccggctt
    13861 gcgcgcgcgt gacggtgcga cgcgtggagc ttcgccttca cctttcgcga agtgcggcgg
    13921 ccacggcgcg tcggcggcgc cttcggcttg ctggcggtcg gcgagctcga gcagcttcgt
    13981 gatgtcgccg ggatgatcgt cgatcgctgc gtgcgcatcg ccgcgtgcct tgaagatggc
    14041 tggcaccgtg cgcagcgtga agtcggcggg attcgcgacg acgaagtcgt cccacgacag
    14101 cggcatcgac acgcgagcgt cttcgcgggc gcgcacggag taggcgcagc atgtcgtgcg
    14161 atcgcgagcg ttctggttgt agtcgaggaa cacgccctgg cgttcttcct tccaccactt
    14221 cgcggtcgcg atcgaaggcg cacgccgctc gacctcacgc gcgaatg
//
LOCUS       DATLBZ010000576         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8604911, whole genome shotgun sequence.
ACCESSION   DATLBZ010000576 DATLBZ010000000
VERSION     DATLBZ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8604911"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VMZ53_28560"
     CDS             complement(<1..1148)
                     /locus_tag="VMZ53_28560"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HUS32500.1"
                     /translation="MNPRSFREYDIRGVAERDLDDTTVRAIGNVIGSKAVGGQVVVGR
                     DPRVHSPRLFAALTDGIRAHAEVIDIGVVPTPVTYFAAHHLKPAASVMITGSHNPPED
                     NGFKMMLGEETLHGHAIAEIRDQVAKLVKEPSPHPKLGMHSRDVTSAYLDHAVSQLKM
                     GHRRCKVVVDAGNGAGGPTAVSLYRRLGFDVIPLYCDLDGRFPNHHPDPTQPENLRDL
                     IAMVQANDAEVGIALDGDADRIGAVDGKGRILWGDQLMIILGKALLGEVPGAKFVGEV
                     KCSQAMYDELAAAGGQVEMWKVGHSLIKARMKETGAHLAGEMSGHMFFAHRWLGFDDA
                     IYSGARLLELLSRGELTLADRASELPVMINTPEIRIDCPDDKKAQVVA"
     gene            complement(1152..2189)
                     /locus_tag="VMZ53_28565"
     CDS             complement(1152..2189)
                     /locus_tag="VMZ53_28565"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HUS32501.1"
                     /translation="MRHAVILAGGSGTRLWPASRRARPKQFLSLSQDNEPLVASAARI
                     GTAAAGRVLIVTADAQVPATRDALPGVEVIAEPVGRNTAAAIGLAAAVLEKRDPEATL
                     LVMPADQHVTDKRAFAAVLESALAAAEAHDAIVTIGITPTRPETGFGYLELAAPAAHG
                     QVVGVERFVEKPDRAKAEEYVASGRFLWNAGIFCATAKRLLAELATHLPTTAHAGREI
                     AREPSTAHALYPTLPSISFDHAVMEKTERVETVPASVGWDDVGSWAAIPAVRGTDDAG
                     NTVAGNAFVLDGTGNVVIADDTTLVAAVGVDDLVIVKSGDAILVIRKDAAQDVRKVID
                     ALSARGLARYL"
     gene            complement(2207..>2725)
                     /locus_tag="VMZ53_28570"
     CDS             complement(2207..>2725)
                     /locus_tag="VMZ53_28570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32502.1"
                     /translation="ILIVTGCMKSGGGASFSTENGTSSGGGGGGGGEGDEASGRNTGD
                     TSGLSLEERKYWRGQEDYLFRSIDAGPENCGVKFSFQWDTDRKTFRDKAEANNNSPYS
                     ICASMVDEVTSLCREGEDEKQSVAAKIKGFRCGYGHPRKATIEDGIVVYMGNHEESNF
                     SDWAKPWLTKNL"
BASE COUNT          420 a          958 c          854 g          493 t
ORIGIN      
        1 tcggcgacga cctgcgcttt cttgtcgtcg gggcagtcga tgcgaatctc cggcgtgttg
       61 atcatcaccg gcagctccga cgcacgatcg gcgagcgtca gctctccgcg cgagagcagc
      121 tcgaggaggc gcgcgcccga atagatcgcg tcgtcgaagc cgagccagcg atgcgcgaag
      181 aacatgtggc ccgacatctc gcccgcgagg tgcgcaccgg tctccttcat gcgcgccttg
      241 atgagcgagt ggccgacctt ccacatctcg acctggccac cggcggcggc gagctcgtcg
      301 tacatcgctt gcgagcactt cacctcgccg acgaacttcg cgccgggcac ctcaccgagc
      361 agcgctttgc cgaggatgat catcagctgg tcgccccaca agatgcggcc cttgccgtcc
      421 accgcgccga tgcgatcggc gtcgccgtcg agcgcgattc cgacctccgc gtcgttcgcc
      481 tgcaccatcg cgatcaggtc gcgcaggttc tcgggctgcg tcggatcggg atgatggttc
      541 ggaaatcgcc cgtcgagatc gcagtacaga gggatgacgt cgaagccgag gcgccggtag
      601 agcgagaccg ccgtcggccc gccagcgccg ttgcctgcat cgacgacgac cttgcagcgg
      661 cggtgtccca tcttcagttg gctgaccgcg tggtcgaggt acgcgctcgt cacatcgcgc
      721 gagtgcattc cgagcttggg atgcggcgac ggctccttca cgagcttcgc gacctgatcg
      781 cggatctcgg cgatcgcatg cccgtgcagc gtctcttcgc cgagcatcat cttgaagcca
      841 ttgtcctcgg gcggattgtg gctgcccgtg atcatcacgc tcgccgctgg cttcaagtga
      901 tgcgccgcga agtacgtgac cggcgttggc acgacaccga tgtcgatcac ttcggcgtgc
      961 gcgcgaatgc cgtcggtgag cgccgcgaac agccgcggcg agtgcacgcg cggatcgcgg
     1021 ccgacgacca cctgcccgcc caccgccttg ctgccgatga cgttgccgat cgctcgaacg
     1081 gtcgtgtcat cgaggtcgcg ctcggcgacg ccgcggatgt cgtattcgcg aaagctgcgc
     1141 gggttcacgg ctcagagata ccgcgcgagg ccacgagccg aaagggcatc gatcaccttg
     1201 cgaacatcct gcgccgcgtc cttgcggatc acgaggatcg cgtcgccgct cttcacgatc
     1261 acgagatcgt cgaccccgac ggcagcgacg agcgtcgtgt catcggcgat gacgacgttg
     1321 ccggtgccat cgagcacgaa cgcattgcct gcgacggtgt tgcccgcatc gtcggtgccg
     1381 cgcaccgcag ggatcgccgc ccacgatccg acgtcgtccc atccgacgct cgcgggcacg
     1441 gtctcgacgc gctcggtctt ctccatcacc gcatggtcga acgagatcga cggcagcgtc
     1501 ggatacagcg catgcgccgt cgacggctcg cgtgcgatct cgcgaccggc gtgcgcggtc
     1561 gtcggcagat gcgtcgcgag ctctgcgagc agccgcttcg cggtcgcgca gaaaatcccc
     1621 gcgttccaca gaaagcgtcc cgacgcgacg tactcctcgg ccttcgcacg atcgggcttc
     1681 tcgacaaacc gctcgacgcc gacgacctga ccatgcgccg ctggcgccgc gagctcgagg
     1741 tacccaaagc cggtctcggg cctcgtcggt gtgatgccga tcgttacgat cgcgtcgtgc
     1801 gcctcggccg ctgcgagcgc gctctcgagc accgccgcga acgcacgctt gtcggtcacg
     1861 tgctggtccg ccggcatgac gagcagcgtc gcctcgggat cgcgcttctc gagcaccgcc
     1921 gccgcgagcc cgatcgccgc cgcggtgttg cgccccacgg gctcggcaat cacttcgacg
     1981 ccaggcaatg cgtcgcgcgt cgccggcacc tgcgcatcgg cggtaacgat gagcactctc
     2041 cctgcggccg ccgtcccgat gcgcgccgca ctcgcgacca acggctcgtt gtcctgcgag
     2101 agcgagagaa actgtttggg ccgcgcgcgc cggctagcgg gccagagccg ggttccgctg
     2161 ccaccggcga gaatcactgc gtgtcgcacg gcggcgaatc tatcacctac aaattcttcg
     2221 tcagccaggg cttggcccaa tcgctgaaat tcgattcttc gtggttaccc atgtagacga
     2281 cgattccgtc ctcgatcgtt gccttgcgcg gatggccata gccgcaccgg aagcccttga
     2341 tcttcgctgc gacggactgc ttctcgtctt caccttcgcg gcacaggctc gtgacctcgt
     2401 cgaccatcga cgcacagatc gagtacgggc tgttgttgtt cgcttcggct ttgtcgcgaa
     2461 acgtcttgcg gtccgtgtcc cactggaagc tgaatttcac gccgcagttc tcggggccgg
     2521 cgtcgatgct gcggaagaga taatcctctt ggccgcgcca gtactttcgc tcctcgagcg
     2581 agagcccgct tgtatcgccg gtgttgcgac cgctcgcctc gtcgccttct cctccaccgc
     2641 caccaccgcc gccgctcgat gttccgttct ccgtcgagaa cgacgcgccg ccgccgctct
     2701 tcatgcagcc ggtgacaatc aagat
//
LOCUS       DATLBZ010000577        17744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3411012, whole genome shotgun sequence.
ACCESSION   DATLBZ010000577 DATLBZ010000000
VERSION     DATLBZ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17744
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3411012"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VMZ53_28575"
     CDS             complement(<1..486)
                     /locus_tag="VMZ53_28575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32503.1"
                     /translation="MLTHNYSYAATLNDSIKINWTVNDLIGGDKRLDFSKPFLPETLA
                     GVRGLSSLSAREKLLLNQIRGNSYLYLFRFVEEYILPFALDEARHVTPGGDPAEVQAL
                     CTFAEEEAKHIHLFHRFAEEFKKGFHTPCEVIGPAADVAKVVLSHSRLGIALLTLHLE
                     WM"
     gene            complement(542..1051)
                     /locus_tag="VMZ53_28580"
     CDS             complement(542..1051)
                     /locus_tag="VMZ53_28580"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HUS32504.1"
                     /translation="MQVHVAKAELWSLPVDAVVNPANSLGIMGGGIGGSLRRQGGDVI
                     QQAAMAAAPIAVGAAIVTTAGQLPSKNVIHVPTMEEPGMMIGAENVRRAARAALIAAD
                     RNNFKVIAIPGIGTDLGNVPIDEAARAIVEEIRAHRRPNPETIYLVDTNEEMVAALEE
                     ALRNAQANL"
     gene            complement(1101..2261)
                     /locus_tag="VMZ53_28585"
     CDS             complement(1101..2261)
                     /locus_tag="VMZ53_28585"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HUS32505.1"
                     /translation="MRAFLLAVVFILGCGGAKKSASAPSGGWQDISVLTRGADPSPQD
                     MGSDALPLYASASTQATPPATAAPPVAEAPAQQEKLVIEAWLQMTTKNVGKAVDDIRS
                     RVELGGGHIVSENVTGSGSSASSATLEFRVPPAQATVITSWLGQLGTVTSKNVKATEV
                     SKTLFDQELALKNLDLTMTRLQALAEKGGPIDQVLAIEKEMTRVRGDIERIKGENRWL
                     VDRVAYATITVSLAREGDEQYDVIPEARIYPGPQLSTLVLIDPGMHQRTRFGAGASVR
                     VHRMLTFDLTVFPRGDGGESRAVLATIGTALYSAYLGYGTRRYLNPYVGARAGYGYFD
                     GTGGAAVAAELGVELYKHEYFLVDTALRALAFIREEGNQPAVQGTLGVSVPF"
     gene            complement(2334..2822)
                     /locus_tag="VMZ53_28590"
     CDS             complement(2334..2822)
                     /locus_tag="VMZ53_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HUS32506.1"
                     /translation="MTTDFKIGEKAVYPAHGVAEVIGIEKKEINTTVCSFYVLKVLAT
                     GMQILVPKDKADQVGLRPVATSSEIEEVFDILREKDVHIDKQTWNRRYRGFMEKIKTG
                     SLFDVAEVYRDLYRLKSSKVLSFGERKMLDNAKQLIVKELAVAKKWAEEKVEQELEKA
                     CA"
     gene            3059..4576
                     /locus_tag="VMZ53_28595"
     CDS             3059..4576
                     /locus_tag="VMZ53_28595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS32507.1"
                     /translation="MRGSIGQLLIAQGAIDAGALSDALARQSGRHPVASELYVLGYCS
                     ERQLCTALSVQTGWPAIVLDESQIRLDVLEHVSIEWARIFSALVVYEDDRSIVVAASR
                     PEDAIVPARELGSSRGKAVELRIALDVTLARTIRVAFRKWKQGETYLIGDEVEKTRKG
                     PHLAIVQPDGGEDVEHRQAQRALAEDAARNILESQEELPPDDHPSSSTLTGDWHVSTI
                     TTSTDNDFSPLLEERTQVITADLDRLGEDTQPTSGHNKALVVDPDPPGRLQLVSELEK
                     LGWQCQAATSGSQAVELLSTIRFELVFADVAAGDVDGLQLCRAIKRSRRLAKTRVVIT
                     TSVVDSGQVPDDVLQRHAADGYIEKPIDMRRLHRMLRELSPDEKVDHEGVLADALGRY
                     HNGDIEGAIAKLRGALVQDPASPKLHFMLANMLQRASRWAEAVDEYEAVVELQPQYFP
                     ALTRLAYLYFRQGLHARAVETWRRALPVCEDPALRRNIELFMRKLVADMSKSDPI"
     gene            complement(4569..9899)
                     /locus_tag="VMZ53_28600"
     CDS             complement(4569..9899)
                     /locus_tag="VMZ53_28600"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32508.1"
                     /translation="MEQHLPHIGQRLGEFEVQEQIGEGGFGKVFRAHQATLGRDAVIK
                     VLANATDALIRDRFIREAQLASKLDHPYAAHVYAFGSEPDGTQWIAMELVRGTTLSDL
                     LKFQGKLPIGRFLPLFQQICQVVQAAHEQGIVHRDLKPANVMVVARAGVLLPKLLDFG
                     IARMYSAEDLAEEVSAAEHRLETLLAVAEARGRGEQPASSKEAAGDTVELDQLDHDLH
                     QLFGHRFAEIASTPREKSAELTSRAVFLGSPPYMAPELWLGPAAAGPQCDVYALGIMA
                     YECLTGKQPYTGRKVLEFARAHLVDNLAEHPAVPAPVLTVLRKAVAKRPRDRYANAMF
                     FAHALEEALREDEAPKQVVARPSPQLAAAVLARAPQPIAETFAAAEVAATPEEAWPRY
                     VALLELLCQWTGVLGLAAWVRLDRPDDNGKMRALASSVCRGEVGPAEWLDLAITATST
                     FAEIRDAFPIPELVDICHGEDGARPVAAQLRGLIELIESLGRKNADGRELTALVSKVA
                     DVMAGFGELFEYDVVVQTVAGCEKWIGPRRKPREIIPLDSGEPTGAPLLVDRHGSVVV
                     ALWPFAKVFAPTPTTEPELFVVAGFGRFGSRLVAKPSGFERHDPELAKWLEELVGTHS
                     ETVHDVEDKPPYRGLASFSVDDSDFFFGRELESQFLANHLRAHSFAAVVGPSGAGKSS
                     FVQAGVMPLLPATWRRVVMRPGSSPLSALRERVLALGGEPPPKTLAAPLVLSWLERTA
                     AQLGATLVIYVDQAEELVTLCRDEAERALFATIAASVRDGSNVRFVLTMRDDFLMRLQ
                     RLPGFAERLAGSIHLLGTPSPNDLKRILVEPARRKGFEFDDAALVDEMVTSVQDNPSA
                     LALLSFTASQLWEQRDRAHKKLRRQVYTSLGGVGGALAQHAEETITQMAPPERELVRE
                     AFRHLVTADGTRAVIGQSELSELLGGAVAGQRVIEKLVNARLAVTSEGATGLTIEITH
                     EALLESWPRLVKWRREDAENARLRDQLRASAKQWDSSGRPRGLLWRGDVLTEYRLWRG
                     RYRGRMTELEESFGSACLREEARGRRGRRALTWSAFLVLSTAAVVLYIFAQRARTSAT
                     HATEKTIEVLQEQGRRDLLDQRPLRAAAPLTRAYSLGGHSSALRFLLATAMTSVENQG
                     AVLRGHEGFIRRIAFSPDGTRIVTYGDDKSVRLWDASTGEQLQALQATRFAQTRDRAL
                     LAVVEASQVSVRKTADFSVVKQWSLEQPITDDNAIAISSDGALVLVGGRDGSVHQFGE
                     TGRQTTHLHDKAVVALVAGDTSKLWASGGADGHVALVHAGEEPTSLVHGTAAVGALAL
                     SHDGKRIASSGHDGIIKIWSVTGTLERTLLGHTERVRDVAFSPDDTRLASCGDDASVR
                     LWKLGSSELPLALSGHTGFVVRVMFDRDGMSLVSSGGDGTVRIWDTISGGAVAALDAH
                     RAEVWDATFDATGERIASASFDLTARVWTIPRRTHRVSIKPVPAGMMGALFSADGKHI
                     ITASPDGTARVYAQTGTLEATLAGKDPLHAAALSPDGMHAMTGTEGGKALLWDVAAAK
                     SKELEGAKDGVNAARFSPDGKRIALGGGDGIVRIADASSGEIVQRLTGHEAAVWGLAY
                     SADGELLASSGDDKTARIWNASTGALVRTIGPTATELNSIAFDAKHQHLATLGGDGVV
                     RIYRVADGTLVTELVAHHDNSTTVEYSADGERIVTASFDHTSIIWDVATKRELLALRV
                     HSDAVMAARFQPGTDRLLTVGLDGQMWLTDIALETRPPADVQTALAARAR"
     gene            10240..10395
                     /locus_tag="VMZ53_28605"
     CDS             10240..10395
                     /locus_tag="VMZ53_28605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32509.1"
                     /translation="MKKRKPKKLELDRKVKPIADDQLSNVSGSKGVPGPIKPQPSIVM
                     TSRNCCW"
     gene            10404..10562
                     /locus_tag="VMZ53_28610"
     CDS             10404..10562
                     /locus_tag="VMZ53_28610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32510.1"
                     /translation="MRKRKPKLELDRTTIKPVDEQALEHVVGGGNPPKHCTPTSIAPT
                     ARNCGGCW"
     gene            complement(10563..12044)
                     /locus_tag="VMZ53_28615"
     CDS             complement(10563..12044)
                     /locus_tag="VMZ53_28615"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUS32511.1"
                     /translation="MRAIAFALVSVLALSPARADSELDTARETPLTDLFAAATKSAPA
                     LESVAFSLAEARAQQATAEGPHDLTLAAIADYQRVKGAPSVPGLPGDSSTTKLYEISL
                     RKLLPTNGLLELVGNAVESDTDLSGMPSDTVTSAVKVRLTQPLLRGVGPAAANRLRES
                     AKLRVSAAGARRAAEARAFAVDLVRAYWELSLAWRKVEIARASADLAKKQQTIVEGGI
                     RTGKLSQSEVYPVQQAFATRQQDIALAELDVIERSIALRRLVGLDVTPEAAALKPAML
                     RPLEKSATAPTDVTGLITRALEISDELRAAREEKRAADAALAGARRERLPRADLELEA
                     GPLGTATTLGASTSKLGDGYTVGARLTIEVPLGNHTGRGIYESDHATAVDATYQLART
                     RNDVAADVTRMVHAARAHLVAAQEGTRAVEAAVANVDAETKKFELGKSTSAEIVRRQD
                     ELQDVRLREAIERTSYEIALAELDAATGAILEKNGLKLIDPWF"
     gene            12059..13957
                     /locus_tag="VMZ53_28620"
     CDS             12059..13957
                     /locus_tag="VMZ53_28620"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HUS32512.1"
                     /translation="MKAVGDFDSEGATQLRAFAFVGADRILLADAALYMRAWLREAAG
                     PNFVIEEVDNARDALERLTADPPRVFVVGAALTDVTGDVVIQAAARNKLLGKRGPAVI
                     LLAPSIEQCAPVDEQVVPVFYRITPQLQPERVRELFSHALSKGKPQAAQKPVGEATQV
                     RNRLVLEHTQRLGSAKDVQSAAAAAVAAVQEITRAERARCLYYDDDTGELWSEAEGID
                     EIKASAGITGFVARAAAQIVLERAADDPAYRPAVDDPAGSGEERLLVTPVTDRDGRVH
                     AILIAIRNAQQGAFAQTEANAVSELAAGWAPFIQQLAAESEVAVANETGPEDDIFRQE
                     AIEHLIRRGHKGDVVRVSPRWINAAFWVVLASVAAAIGFAAVARIHQYTEGPAVIRVT
                     GRTDLIAYDGGSVTSVEVAAGQAVKENQVLVRLHDTEQANRMRGLEADFERKLVAYLQ
                     SPADPNVRQALSSLVSERESGKANLEARVIRAPHDGIVREVLVNVGQRVDPGKVVVAV
                     ARKDVPEGLSALAFLPGGDRPRLHVGQKLRMTLPGYRGATVQGTVRAISSDVIGSADA
                     KARYLGDRLGDSLPISGTVVVVEAQLPKTFEADGQRFDLHDGMVGTSEVQLDSKTVLE
                     TAMPGLKF"
     gene            13957..16167
                     /locus_tag="VMZ53_28625"
     CDS             13957..16167
                     /locus_tag="VMZ53_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HUS32513.1"
                     /translation="MQGEDLFPSLQRLQGQRKRGRVPFVQQTEAADCGPASLAMVLKF
                     LGRDAMLDEVREAIGGGRDGTDAAGIIRGASHYGLVGRGLSLDVDNLRFLPAGSILHW
                     EFNHFVVFERMTKAGAVIVDPARGTRTIPEKQLREAFTGVALVFEPGGAFEKRRAGSG
                     RFRWYMSQVFTQTKLVTRIFVTSLLLRVFALALPLLVALVVDRVIPRSDYGLLTIVAL
                     GLGGMLVFQALTSLIRSHLFLQLRTNLDTRLTLGFVDYMSRLPFDFFQRRSAGDLMMR
                     VNNNATVRELLTSNMLSTLLDGILVLGYLGLVVWLAPMMAAVVGALGVTQVLLFYATR
                     RRYRELLANSLDAQARSQSYLVELFAGMATLKASSAESRAVERWSNLYVDELNVSLER
                     GRLSAVVDAVRGFLETASPMVILGVGAVEVIRGEMTLGHMLAVNALAIALLAPLSSLV
                     QSALQLQLLGGYMDRIDDVLAQAPEQRGDQVAKPPRLTGRVAMQGVSFRYAERAPFVV
                     QNVTLDIRAGTTVAIVGRSGCGKSTLAGLIAGMYQPSEGKILFDGHDASRLDLRALRR
                     QLGIVFQQPYLFQGSIRSNIALTDPAIPLDRVVGAARMACIHDDIDRMPMAYDTIIAD
                     GGSSLSGGQRQRLAIARALVHNPSILVLDEATSALDAETERRVSENLARLNCTRIVLA
                     HRLSTIIAADMIIVLDRGEIVEAGTHHELLARGQHYRNLVAGQLRQEQGQEVAS"
     gene            16164..17051
                     /locus_tag="VMZ53_28630"
     CDS             16164..17051
                     /locus_tag="VMZ53_28630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUS32514.1"
                     /translation="MRALWLITLLCACHGDTRERVDEKAAPEPLSVGKTTQTDTPKSA
                     GYIGVLTPKDVVEVTAPFTTNVKQFQVKLGEQVEKGTELALLDRQPLEEQLAVAKAEL
                     KGSEAEVGAAKTTAGAAAAALRREKAGQRAGIASKADVSNAAYKASEASSLVSKAVAA
                     VEQQKARIKALESKLTQMTLTAPIAGRVSLRYVEPGGRVEEGKPIVRVISSDELFVKF
                     AIPSEQAGRISPGDVVDVVIEGRDDKLAAKVKTVAPELDPVAQMIVAEAELTGTVPAK
                     LQSGMVCRIVVADGAKHAN"
     gene            17051..>17744
                     /locus_tag="VMZ53_28635"
     CDS             17051..>17744
                     /locus_tag="VMZ53_28635"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HUS32515.1"
                     /translation="MMPLLTDSAAARARDAVIAIDAAIRDDESGNDGYAALFSAYVAT
                     LVPDGDAQQERMLDRLVERLGQPYARAGLFGGLAGDGWVLCHIGDECEPLLVQIDRQL
                     HAVLPTLADFDLISGSAGIAIYFAERLHQGAEARDALATTFAHLASGWRAAPGDDCGL
                     AHGTPGVIAAVTVAASRGIVDAVALRDEALAWLSRARLADGAYPTKLGGRPARTAWCY
                     GDPGIVAGLWNAG"
BASE COUNT         2938 a         5833 c         5954 g         3019 t
ORIGIN      
        1 catccactcg aggtggagcg tcagcagcgc gatgccgagg cggctgtgcg agagcacgac
       61 cttcgcgaca tcggccgcag gaccgatgac ttcgcacggc gtgtggaagc ctttcttgaa
      121 ttcctcggcg aagcgatgga acaggtggat gtgcttcgcc tcttcttcgg cgaacgtgca
      181 gagggcctga acttcggcgg gatcgccacc gggcgtcacg tgacgagctt catcgagcgc
      241 gaacggcagg atgtattctt cgacgaagcg gaagaggtac aggtagctgt tgccgcggat
      301 ctggttgagc agcagcttct cgcgcgcgct cagcgacgac aggccgcgga caccggcgag
      361 tgtctccggc aggaacggct tcgagaagtc gaggcgcttg tcgccaccga tcaggtcgtt
      421 gacggtccag ttgatcttga tcgagtcgtt cagcgtcgct gcgtagctgt agttgtgcgt
      481 cagcatgtat cacccgttcc cctgttgatg aacgggcagc ctacgccaac gagccggcgg
      541 tctataggtt tgcttgtgcg tttcggagcg cttcttcgag cgccgcgacc atctcttcgt
      601 tcgtgtcgac gagatagatc gtctccggat tcgggcgacg gtgcgcgcgg atctcttcga
      661 cgatcgcgcg ggcggcttca tcgatcggca cgttgccgag gtcggtgccg atgcccggga
      721 tcgcgatgac tttgaagttg ttgcggtcgg ctgcgatgag cgcggcgcgg gcggcgcggc
      781 gcacgttctc ggcgccgatc atcatgcccg gctcttccat cgtcgggacg tggatgacgt
      841 tcttcgaggg gagctggcca gcggtcgtga cgatggcggc gccgacggcg atcggggcag
      901 cggccatcgc ggcctgctgg atcacgtcac caccctggcg gcggagcgag ccgccgatgc
      961 cgccacccat gatgccgagc gagttggccg gattgacgac cgcgtcgacg ggcaacgacc
     1021 acagctctgc tttggcaacg tgaacctgca tgcagcccgg accgttacca cgcggcggct
     1081 attgccttca agcacccgct ttagaacggc accgagacgc ccagcgtgcc ttggacggca
     1141 ggctggttgc cctcttcgcg gatgaaggca agcgcgcgaa gcgccgtgtc gacgaggaag
     1201 tattcgtgtt tgtagagctc gacgccgagc tcggccgcga ccgccgcacc gcccgtcccg
     1261 tcgaagtagc cgtaccccgc gcgcgccccg acgtaggggt tcaggtaccg gcgcgtgccg
     1321 tatccgagat acgcggagta gagcgcggtg ccgatcgtcg cgaggaccgc ccgcgactcg
     1381 ccgccatctc cgcgcgggaa cacggtcagg tcgaacgtca gcatgcgatg cacgcgcacg
     1441 ctcgctccgg cgccgaaccg cgtgcgctgg tgcatgcccg gatcgatcag cacgagcgtc
     1501 gacagctgcg ggccgggata gatgcgggct tcggggatca cgtcgtactg ctcgtcgcct
     1561 tcgcgcgcga ggctgacggt gatcgttgcg tacgcgacgc gatcgacgag ccagcggttc
     1621 tcgcccttga tgcgttcgat gtcgccgcgc acgcgcgtca tctccttttc gatcgcgagc
     1681 acctgatcga tcgggccgcc cttctcggcg agggcctgca agcgcgtcat cgtcagatcg
     1741 aggttcttca gcgcgagctc ttgatcgaag agcgtcttac ttacctcggt ggcctttacg
     1801 ttcttcgacg tcaccgtgcc gagctggccg agccacgacg tgatcaccgt ggcctgcgcc
     1861 ggtgggacgc gaaactcgag cgtcgcgctc gacgccgacg agccggagcc cgtcacgttt
     1921 tcgctcacga tgtgaccacc gccgagctcg acgcgcgagc gaatatcgtc gacggctttg
     1981 ccgacgttct tcgtcgtcat ctgcagccac gcttcgatga cgagcttctc ctgctgagca
     2041 ggcgcttcgg ccaccggcgg cgcagcggtt gcgggcggcg ttgcttgcgt cgaggccgat
     2101 gcgtacagcg gcagtgcatc gctgcccatg tcctgcggcg atgggtctgc gccgcgcgtc
     2161 aggaccgaga tgtcctgcca gccgcccgac ggcgcagatg ccgatttctt tgcaccgcca
     2221 caaccgagga tgaaaacgac cgccaaaaga aaagcgcgca tgccggtcgg gacacgcgcg
     2281 catcgggtga gttgcctctg taagttttag gcagctcggt cgttacgaga ggttcaggcg
     2341 cacgccttct cgagctcttg ctcgaccttc tcctcggccc acttcttcgc gacggcgagc
     2401 tctttcacga tgagctgctt cgcgttgtcg agcatcttgc gctcgccgaa cgagagcacc
     2461 ttgctgctct tgaggcgata gagatcgcga tagacctcgg cgacgtcgaa cagcgagccc
     2521 gtcttgatct tctccatgaa cccgcgatag cggcggttcc acgtctgctt gtcgatgtgg
     2581 acgtccttct cgcgaaggat gtcgaagacc tcttcgatct cggacgaggt cgcaacgggg
     2641 cggaggccga cttggtctgc tttgtccttg gggacgagaa tctgcatgcc ggtcgcgagg
     2701 accttgagca cgtagaagga gcaaacagtc gtgttgatct ccttcttctc gattccgatc
     2761 acttcggcta ccccgtgcgc gggatagacc gccttttcgc caatcttgaa gtcggttgtc
     2821 atgaagtact ccgattaagc aatgccggtg cccttcggcg cggcgtctag taacgctggg
     2881 ttagcgtcgt tgcccggtct cgggctgcgc caagctggca cacgggatcc gaggctgacc
     2941 caacgggacg cggaggatat caatcatcgc taggatctcc agccaaatcg acatccggcg
     3001 gatcggctag ccggcacaga ttcgagattt cgaagggtta ctggtaccct tcggaccggt
     3061 gcgcggctcg attggtcagc tcctcattgc gcagggcgcg atcgatgcgg gagcactttc
     3121 ggacgccttg gcgcggcaga gcgggcggca cccggtggcc tccgagctgt acgtcttggg
     3181 gtactgctcc gaacgtcagc tctgcacggc gctgtcggtc cagaccgggt ggccggcgat
     3241 cgtgttggac gagtcgcaga tccgcctgga cgtgctcgag cacgtgtcga tcgaatgggc
     3301 gcgaatcttc tcggcgctcg tggtctacga agacgaccga agcatcgtcg tcgcggcatc
     3361 gcggcccgaa gacgcgatcg tgccggcgcg cgagctcggt tcgagccggg gcaaggcagt
     3421 cgagctccgc atcgcgctcg atgtcacgct cgcacggacg atccgcgtcg cgtttcgcaa
     3481 gtggaagcag ggcgagacgt acctcatcgg cgatgaagtc gagaagacgc gcaagggccc
     3541 gcatctcgcg atcgttcagc ccgatggcgg cgaagatgtc gagcaccgtc aggcgcagcg
     3601 cgcgctcgcc gaagatgcag ctcgaaatat tttggagagc caggaagagc tgcctcccga
     3661 tgatcatcca tcgagctcga cgctgaccgg cgactggcac gtcagcacga tcacgacgtc
     3721 gacggacaac gacttcagcc cgctgctcga ggagcgcacg caggtcatca cggccgatct
     3781 cgatcggctc ggcgaagaca cgcagccaac gagtggacac aacaaggcgc tcgtcgtcga
     3841 tcccgatccg ccgggacgcc tccagctcgt gagcgagctc gagaagctcg gttggcaatg
     3901 tcaggccgcg acgagcggct cgcaggcggt cgagctgctg tcgacgatcc gcttcgagct
     3961 cgtgttcgcg gatgtcgccg caggcgacgt cgatgggctc cagctgtgtc gcgcgatcaa
     4021 gcgcagccgc cgcctcgcga agactcgcgt cgtgatcacc acgagcgtcg tcgattcggg
     4081 ccaggtgccc gacgacgtcc tccagcgcca cgctgccgac ggctacatcg agaagccgat
     4141 cgacatgcgg cgcttgcatc gcatgctgcg cgagctgtcg cccgacgaga aagtcgatca
     4201 cgagggcgtg cttgccgatg cgctcggccg ctatcacaac ggcgacatcg aaggtgcgat
     4261 cgcgaagctg cgcggtgcgc tcgtgcagga tccggcgagc cccaagcttc acttcatgct
     4321 cgcgaacatg ctgcagcgcg cgtcgcgctg ggcagaagcc gtcgacgagt acgaggccgt
     4381 cgtcgagctg cagccgcaat acttccccgc gctcacgcgg ctcgcatatc tctacttccg
     4441 gcagggtctc cacgcgcgcg cggtcgagac gtggcggcgt gcactgcccg tgtgcgagga
     4501 ccctgcgctg cgccgcaaca tcgagctgtt catgcgcaag ctcgtcgcgg atatgtcgaa
     4561 gtccgaccct atctagctcg cgcggcgaga gccgtctgca catcggcagg cggacgcgtc
     4621 tcgagcgcga tgtcggtcag ccacatctgg ccgtcgagac caacggtgag caggcgatcc
     4681 gtcccgggct ggaagcgcgc cgccatgacg gcatcgctgt ggacgcgcag tgcgagcagc
     4741 tctcgcttcg tcgcgacgtc ccagatgatc gacgtgtgat cgaagctcgc cgtcacgatg
     4801 cgctcgccat cggccgagta ctcgacagtc gtgctgttgt cgtgatgcgc gacgagctcg
     4861 gtcacgagcg taccgtcggc aacgcggtag atacgcacga cgccgtcgcc gccgagcgtc
     4921 gcgagatgct ggtgcttggc gtcgaacgcg atcgagttga gctccgtcgc cgttggcccg
     4981 atcgtgcgga cgagcgcgcc cgtcgaagcg ttccagatgc gcgcggtctt gtcgtcgccg
     5041 gagctcgcga gcagctcgcc gtcggcggag taggcgaggc cccacaccgc cgcctcgtgg
     5101 ccggtcagcc gctgcacgat ctcgccgctc gaagcgtccg cgatccggac aatcccgtcg
     5161 ccgccgccca acgcgatgcg cttgccgtcg ggactgaaac gcgcggcgtt gacaccatcc
     5221 ttcgcgcctt cgagctcttt gctcttggcg gccgcgacat cccacagcag cgccttgccg
     5281 ccctcggttc cggtcatcgc gtgcatgccg tcgggcgaga gcgcggcggc gtggagagga
     5341 tccttgcctg caagtgtggc ctcgagcgtg ccggtctgcg cgtacacgcg tgcagtgccg
     5401 tcggggctcg cagtgatgat gtgcttgccg tcggcggaga acagcgcgcc catcatgccg
     5461 gcaggcacgg gcttgatcga cacgcgatgc gtgcgccgcg gaatcgtcca cacgcgcgct
     5521 gtgagatcga agctcgcgct cgcgatgcgc tcgcccgtgg catcgaacgt cgcgtcccat
     5581 acctccgcgc gatgagcatc gagcgcagcg acggcgccgc cactgatcgt gtcccagatg
     5641 cgcaccgtgc cgtcgccgcc ggaggacacg aggctcatgc catcgcgatc gaacatcacg
     5701 cggacgacga atcctgtgtg gcccgagagc gcgagcggca gctcactcga gccgagcttc
     5761 cagagccgca cgctggcgtc gtcgccgcac gaagcgagcc gggtgtcgtc gggagaaaac
     5821 gcgacgtcgc gaaccctctc ggtatgcccg agcagcgtgc gctcgagcgt acccgtcacg
     5881 ctccagatct tgatgatgcc gtcgtgaccc gacgacgcga tccgcttgcc gtcgtgactc
     5941 agcgcgagcg cgccgaccgc agcggtgccg tgaacgagcg acgtcggttc ctcgcccgca
     6001 tgaacgagcg ccacgtggcc gtccgcgcca ccgctggccc acagcttgga cgtgtcgccc
     6061 gcgacgagcg cgacgacggc cttgtcgtgc aggtgcgttg tctgccgccc tgtctcgccg
     6121 aactgatgga cgctgccgtc ccgtccgccg acgagcacga gcgcaccgtc gctgctgatc
     6181 gcgatcgcgt tgtcgtccgt gatcggctgc tcgagactcc attgtttgac gaccgagaaa
     6241 tctgcggtct tgcgaacgct cacctggctg gcttcgacga ccgcgagcaa ggcgcgatca
     6301 cgggtctgcg cgaagcgcgt tgcctgcagc gcctgcagct gctccccggt ggacgcatcc
     6361 cagagccgca cggacttgtc gtcgccatat gtgacgatgc gcgttccgtc gggcgagaac
     6421 gcgatgcggc ggatgaagcc ctcgtggccg cggagcacgg cgccttggtt ctcgacgctc
     6481 gtcatcgcgg tcgcgagcaa gaagcgcagt gcggagctgt gaccgccgag cgagtacgcg
     6541 cgcgtgagcg gcgcagctgc acgcagcggg cgctgatcga gcagatcgcg acgaccttgc
     6601 tcctgcaaca cttcgatcgt cttctccgtc gcgtgcgtcg cgctcgtgcg tgcgcgctgc
     6661 gcgaagatgt acagcacgac ggccgccgtc gagaggacga ggaatgcgct ccacgtgagc
     6721 gcgcgcctgc cccggcgacc tcgcgcctct tcgcgcaagc acgccgaacc gaagctctcc
     6781 tcgagctcgg tcattcgacc gcgatagcga cctcgccaca gccgatattc ggtgagcaca
     6841 tcgccgcgcc atagcaggcc gcgaggccga ccgctcgagt cccactgctt cgcgctcgca
     6901 cgcagctgat cgcggaggcg ggcattctcg gcgtcttcgc ggcgccactt gacgaggcgt
     6961 ggccacgatt cgagcaacgc ttcgtgcgtg atctcgatcg tgaggcccgt tgcgccttca
     7021 ctcgtaacgg cgaggcgagc gttgacgagc ttctcgatca cgcgttgacc ggcgactgcg
     7081 ccgcccagca actccgagag ctcgctctgt ccgatcacgg cgcgcgtgcc gtcggcggta
     7141 acgagatggc ggaatgcttc acgcacgagc tctcgctccg gcggtgccat ctgcgtgatc
     7201 gtctcttcgg cgtgctgggc gagtgcgcca ccgacgccgc cgagcgacgt gtagacctga
     7261 cgacgcagct ttttgtgcgc gcgatcgcgt tgctcccaca actgcgacgc ggtgaacgac
     7321 agcagcgcga gcgcgcttgg attgtcctgc acgctcgtga ccatctcgtc gacgagcgcg
     7381 gcgtcatcga actcgaagcc tttgcgacgc gcgggctcga cgaggatgcg cttgagatcg
     7441 ttcggcgacg gcgtaccgag taggtggatg ctgccggcga gacgctccgc gaacccaggc
     7501 aggcgctgca gtcgcatcag gaagtcgtcg cgcatcgtca gcacgaaccg cacgttcgag
     7561 ccatcgcgga cgctcgctgc aatcgttgcg aataacgcgc gttcggcttc gtcgcggcac
     7621 agcgtcacga gctcttcggc ctggtcgaca taaatgacga gcgtcgcgcc gagctgcgcg
     7681 gcggtgcgtt cgagccacga cagcacgaga ggagcagcga gcgtcttcgg cggcggctcg
     7741 ccaccgagtg caagcacgcg ctcgcgcagt gcgctcagcg gcgacgaacc ggggcgcatc
     7801 acgactctgc gccatgtcgc gggcaagagc ggcatcacgc ctgcctgtac gaacgagctc
     7861 ttaccagcgc ccgacggacc gacgaccgcg gcaaacgagt gcgcgcgcag atggttcgcg
     7921 aggaactgac tctcgagctc acgaccgaag aagaaatcgc tatcgtcgac ggagaacgac
     7981 gcgaggccgc gataaggcgg tttgtcctcg acgtcgtgca cggtctcgga gtgcgtgccg
     8041 actagctcct cgagccactt cgctagctcg gggtcgtgac gctcgaaccc gctcggcttg
     8101 gcgacaaggc gcgagccgaa ccgaccaaag ccagcgacca cgaacagctc cggctcggtc
     8161 gtcggcgtcg gcgcgaagac cttcgcgaac ggccacagcg cgaccacgac ggagccgtgg
     8221 cgatcgacga gcagaggcgc accggttggc tcgccactgt cgagcgggat gatctcgcgc
     8281 ggtttgcggc gcgggccgat ccacttctcg cagccggcca cggtttgcac gacgacgtcg
     8341 tactcgaaca gctcgccaaa ccccgccatc acgtcggcga ctttcgagac gagggccgtc
     8401 agctcgcgcc cgtcggcgtt cttgcgaccg agcgactcga tgagctcgat gaggccgcgc
     8461 agctgcgccg cgacggggcg agcgccgtct tcgccatgac aaatatcgac gagctctgga
     8521 atcgggaacg catcgcggat ctctgcgaac gtcgacgtcg cggtgatcgc gagatcgagc
     8581 cactccgcgg ggccgacttc gccgcggcaa acgctcgacg cgagcgcgcg catcttgccg
     8641 ttgtcgtccg ggcgatcgag ccgcacccac gccgcgagac cgaggacgcc cgtccattgg
     8701 cagagcagct cgagcagcgc gacatagcga ggccacgctt cttccggtgt cgccgccacc
     8761 tcggcagcag cgaatgtctc cgcgatcggc tgcggcgcac gcgcgaggac agccgctgcg
     8821 agctgcggcg acggccgcgc gacgacctgt tttggcgcct cgtcctcgcg cagcgcttct
     8881 tcgagtgcgt gcgcgaagaa catcgcgttc gcgtagcgat cgcgtggccg cttggcgacg
     8941 gccttgcgca acactgtgag caccggtgcc ggcacggctg gatgctccgc gagattgtcg
     9001 acgaggtgcg cgcgtgcaaa ctcgagcacc tttcggccgg tgtacggctg ctttccggtc
     9061 aggcactcgt aggccatgat tccgagcgcg tagacatcgc attgtggtcc tgccgcggcg
     9121 ggcccgagcc acagctccgg cgccatataa ggaggcgagc cgaggaagac ggcgcggctc
     9181 gtcagctcgg cggatttctc gcgaggtgtc gacgcgatct cggcaaagcg gtgaccgaac
     9241 agctgatgga gatcgtgatc gagctgatcg agctcgacgg tgtcgcctgc ggcctccttt
     9301 gatgaggctg gctgttctcc gcgcccccgc gcttcggcaa ctgcgaggag cgtctccagc
     9361 cggtgctccg cggccgacac ttcctccgcg agatcctcgg ccgagtacat tcgcgcgatg
     9421 ccgaagtcga ggagcttggg caacagcacg ccggctcgcg cgacgaccat gacgttcgcc
     9481 ggcttgagat cgcggtgcac gatcccttgt tcgtgcgcgg cctgcacgac ctgacagatc
     9541 tgttggaaca gcggcaggaa gcgcccgatc ggcagcttgc cttggaactt gagcaagtca
     9601 gacagcgtcg tgccgcgcac gagctccatc gcgatccact gcgttccatc gggctcgctg
     9661 ccgaacgcgt agacgtgcgc ggcgtacgga tgatccagct tgctcgcgag ttgcgcttcg
     9721 cgaatgaagc gatcgcggat caacgcatcg gtcgcgttcg cgagcacctt gatgacggcg
     9781 tcgcgtccga gcgtggcttg gtgggcccga aagaccttac cgaagccacc ctcgccgatc
     9841 tgctcctgga cttcgaactc ccccagtcgc tggccgatat gggggaggtg ttgctccacc
     9901 aatccagact gtaactgcac aacgccggtc gttgaaggtc agtggttgca cttcgagtgc
     9961 atgcacaaat tgtaaaggcg tgcatgtcgc acctcgaacg atcagccaca tgagcgcggc
    10021 atcacccttg ctctagtcca actcggggat agggcgccgc gcacctacac cgagggtcat
    10081 atgaagaaac agaagtccaa aaagctccag ctcgatcagg tcgtcatcag gccactcgac
    10141 gagaaggtgc tggagcatgt gacgggtgga gcgccacgtc caaaccacga gaacagcatc
    10201 gtcccaacgg cgcgtagctg ctgtttctaa agggggagta tgaagaagcg gaaaccaaag
    10261 aagctggagc tcgatcgcaa ggtgaagccg atcgcggatg atcagctgtc gaacgtgagc
    10321 ggcagcaagg gcgttcccgg gcccatcaag ccgcaaccga gcatcgtgat gacttcccga
    10381 aactgctgtt ggtaagggga tcgatgcgta agcgaaaacc aaagctcgag ctcgatcgca
    10441 cgacgatcaa accggtcgac gagcaagcgc tcgagcacgt cgtcggcggt ggcaatccgc
    10501 cgaagcactg cacgccaacg agcattgcgc ccaccgcacg caactgcggc ggttgctggt
    10561 agctagaacc agggatcgat cagctttagg ccattcttct cgaggatcgc gccggtcgct
    10621 gcatccagct cggcgagcgc gatctcgtag ctcgttcgtt cgatcgcttc gcggagccga
    10681 acgtcctgga gctcgtcctg gcggcgcacg atctcggcgc tcgtcgactt tccgagctcg
    10741 aacttcttcg tctcggcgtc gacattcgcg accgcggcct cgacggccct cgtgccttcc
    10801 tgggcggcga cgaggtgagc acgcgctgca tgcaccatgc gcgtgacatc cgcggcgacg
    10861 tcgttgcgcg ttcgcgcgag ctgatacgtc gcgtcgacgg cggttgcgtg atcggactcg
    10921 tagatgccgc ggccggtgtg gttgccgagc gggacctcga tcgtcaggcg cgcgccgacc
    10981 gtgtagccgt cgccgagctt gctcgtcgaa gcgccgagcg ttgtcgcggt gccaagaggg
    11041 cccgcttcga gctcgagatc ggcgcgcggc agacgctcgc ggcgcgcacc ggcgagtgcg
    11101 gcatcggcgg cacgcttctc ttcgcgcgcg gcccgcagct cgtcgctgat ctcgagcgca
    11161 cgcgtgatga gaccggtgac gtcggtaggc gcggtcgccg atttctcgag agggcgcagc
    11221 attgccggtt tcaacgcggc cgcttccggc gtgacgtcga gaccgacgag ccggcgcagc
    11281 gcgatcgatc gttcgatcac gtcgagctcg gcgagcgcga tgtcctgttg ccgcgtcgcg
    11341 aacgcttgct gcaccggata gacctcgctc tgcgacagct tgccggtgcg aatgccgccc
    11401 tcgacgatcg tctgctgctt cttcgcgaga tcggcgctcg cccgcgcgat ttcgaccttg
    11461 cgccacgcca atgacagctc ccagtaggcg cgcacgagat cgacggcaaa cgcccgagct
    11521 tctgccgcgc gccgcgcccc cgctgccgag actcgcagct tcgccgattc gcgcagccgg
    11581 ttcgcggcgg cgggcccgac gccgcgtagc agcggctggg tcaggcgcac cttcacggcg
    11641 gaggtcacgg tgtccgacgg catgccggag agatcggtgt ccgactccac ggcattgccg
    11701 acgagctcga gcaggccatt cgtcggcagg agcttgcgca gcgagatctc gtagagcttc
    11761 gtcgtcgagg aatcgcccgg caggcccggc acactcggcg cacctttgac gcgctggtag
    11821 tcggcgatcg ctgccagcgt caggtcgtgc gggccctccg cggtcgcttg ctgggcgcgc
    11881 gcctctgcga gcgagaacgc gacgctctcg agcgcgggcg ccgacttcgt tgcggcggcg
    11941 aacaggtcgg tcagcggtgt ctcgcgggcc gtgtccagct cggaatctgc ccgtgcgggt
    12001 gagagggcga ggaccgatac gagtgcgaag gcgatcgcgc gcatggggcg atgatacgat
    12061 gaaggcagtg ggggacttcg actccgaggg agcgacccag ctccgtgcgt tcgcattcgt
    12121 gggcgcagat cggatcctgt tggccgatgc agcgctctat atgcgcgcct ggctgcgcga
    12181 ggcagctggc ccgaacttcg tcatcgaaga ggtcgacaac gcgcgcgacg cgctcgagcg
    12241 gctgactgcc gatcccccac gcgtgttcgt ggtcggcgcc gcgctcaccg acgtcacggg
    12301 cgatgtcgtg attcaggcgg ccgctcgcaa caagctgctc ggcaagcgtg ggccggccgt
    12361 catcttgctc gctccgtcga tcgagcagtg cgcgcccgtc gacgagcaag tcgtgccggt
    12421 gttctatcgg atcacgccac agctgcagcc cgaacgagtc cgcgagctgt tttcgcacgc
    12481 gctctcgaag ggcaagccgc aggccgcaca gaagcctgtc ggcgaagcta cgcaggtccg
    12541 caatcgactc gtcctcgagc acacgcagcg gctcggctcc gcgaaagatg tgcagagcgc
    12601 tgcggccgca gctgtcgcgg ccgtccaaga gatcacgcgg gccgagcgtg cgcggtgcct
    12661 ctattacgac gacgacaccg gcgagctgtg gtcggaggcc gagggcatcg acgagatcaa
    12721 agcatccgcc ggcatcaccg gattcgtcgc gcgtgcagcc gcgcagatcg tgctcgagcg
    12781 cgccgccgat gacccggcct accgtcccgc cgtcgacgat ccggcaggca gcggcgaaga
    12841 gaggttgctc gtcacgccgg tcaccgatcg cgacggccgc gtgcacgcga tcctcatcgc
    12901 gattcgcaat gcgcagcagg gggcgttcgc gcaaaccgaa gcaaatgcgg tctccgagct
    12961 cgccgcgggg tgggcgccgt tcatccagca gctcgcggcc gaatccgagg tggcggtcgc
    13021 aaacgagacg gggcccgagg acgacatctt tcggcaggag gcgatcgagc acctgatccg
    13081 ccgcggccac aaaggcgacg tcgtgcgcgt ctctcctcgc tggatcaacg ccgcgttctg
    13141 ggtcgtgctc gcgtccgtcg cagccgcgat cggatttgca gcggtcgcgc ggatccacca
    13201 gtacaccgag ggacccgcgg tgatccgggt gacgggccgc accgatctca tcgcgtatga
    13261 cggcggcagc gtcacctccg tcgaggtcgc agccgggcag gccgtgaagg agaaccaggt
    13321 cctcgtgcgc ttgcacgaca ccgagcaggc caaccgcatg cggggactcg aggcagactt
    13381 cgagcgcaag ctcgtcgcgt atctgcagtc gcctgccgat ccgaatgtgc ggcaggcact
    13441 gtcgtcgctc gtctcggagc gcgagagcgg caaggcaaac ctcgaagcgc gcgtcattcg
    13501 cgcaccgcat gacggcatcg tccgcgaggt cctcgtcaat gtcgggcagc gcgtcgatcc
    13561 gggaaaggtc gtcgtcgccg tcgcacgcaa agatgtgccg gagggcctct cggcgctcgc
    13621 gttcttgccg ggcggcgatc gaccacggct gcacgtcggc cagaagctgc gcatgacgct
    13681 gcccggctat cgcggcgcca ccgtgcaagg gaccgtgcgt gcgatcagct ccgatgtgat
    13741 cggatccgcc gatgcaaagg cgcgctatct cggcgaccgg ctcggcgata gcctgccgat
    13801 cagcggcacg gtcgttgtcg tcgaagcgca gctgccaaag acgttcgaag ctgacggcca
    13861 gcgattcgat ctccacgacg gcatggtcgg aacgagcgaa gtgcagctcg acagcaagac
    13921 ggtgctcgag acggcgatgc cggggttgaa gttctgatgc aaggcgaaga tctcttccca
    13981 tcgctccagc gcctgcaagg tcagcgcaag cgcggacgtg tgccgttcgt gcagcagacc
    14041 gaagccgcgg actgcgggcc tgcgtcgctc gcgatggtgc tcaagttcct cggccgcgat
    14101 gcgatgctcg acgaagttcg cgaggcgatc ggcggcggac gcgacggtac cgacgctgcg
    14161 ggcatcatcc gcggtgcatc gcactatggc ctcgtcgggc gcggcctctc gctcgacgtc
    14221 gacaacctgc gctttctacc ggcgggctcg atcctgcact gggagttcaa tcacttcgtc
    14281 gtattcgagc gcatgacgaa ggccggcgcg gtcatcgtcg acccggcgcg cggcacgcga
    14341 acgattccgg agaagcagct gcgcgaagcg ttcacgggtg tcgcgctcgt gttcgagccc
    14401 ggcggcgcgt tcgagaagcg tcgtgccggc agcgggcgct tccgttggta catgagccag
    14461 gtcttcacgc agaccaagct cgtcacccgc atcttcgtga cgtcgctgct gctgcgcgtg
    14521 ttcgcgctcg cgctgccgct cctcgtcgcg ctcgtcgtcg atcgcgtgat cccgcgcagc
    14581 gactacggcc tcttgacgat cgtcgcgctc ggcctcggcg gcatgctcgt gttccaggcg
    14641 ttgacgagcc tcatccgttc gcacttgttc ctccagctgc gcacgaatct cgatacacgc
    14701 ctgacgctgg gcttcgtcga ttacatgtcg cggctgccgt tcgatttctt ccagcgccga
    14761 tccgcgggcg acctgatgat gcgggtcaac aacaacgcga cggtccgcga gctgttgacg
    14821 tccaacatgc tatcgacgct gctcgacggc atcctcgtgc tcggctacct cggcctcgtc
    14881 gtgtggctgg cgccgatgat ggcagcggtc gttggcgcgc tcggcgtcac gcaagtgttg
    14941 ctgttctatg cgacgcgccg ccgatatcgc gagttgctcg cgaattcgct cgatgcgcag
    15001 gctcgctctc agtcgtatct cgtcgagcta ttcgccggca tggccacgct caaggcatcg
    15061 tcggcggaga gccgcgccgt cgagcgctgg tccaatttgt acgtggacga gctcaacgtg
    15121 tcgctcgagc gcgggcgcct gtcggcggtc gtcgatgcag tgcgcggctt cctcgaaaca
    15181 gcgtcgccga tggtgatcct cggcgtcggc gcggtcgaag tgatccgtgg cgagatgacg
    15241 ctcggccaca tgctcgccgt caacgcgctc gcgatcgcgt tgctcgcgcc gctctcgtca
    15301 ctcgtgcaga gcgcgctgca gttgcagctg ctcggtggat acatggaccg catcgacgac
    15361 gtgctcgccc aggcacccga gcagcgcggc gatcaggtcg cgaagccgcc gaggctcacc
    15421 ggccgcgtcg cgatgcaagg cgtgtcgttc cgctatgccg agcgcgcgcc gttcgtcgtc
    15481 cagaatgtga cgctcgatat tcgtgcgggg acgacggtcg cgatcgtggg gcgatccggc
    15541 tgtggcaagt cgacgctcgc cggcctgatc gcgggcatgt accagccgag cgagggcaag
    15601 atcttgttcg acgggcacga cgcgagccgg ctcgacctgc gtgcgctgcg ccgccagctc
    15661 ggcatcgtgt tccagcagcc gtacctgttc cagggctcga ttcgcagcaa catcgcgctg
    15721 accgatcctg cgatcccgct cgatcgcgtg gtcggtgccg cgcgtatggc gtgcatccac
    15781 gacgacatcg atcgcatgcc catggcgtac gacacgatca tcgccgacgg cggctcgtca
    15841 ctctcgggtg gtcagcgcca gcggctcgcg atcgcgcgtg cgctcgttca caacccgtcg
    15901 atcctcgtac tcgacgaagc gacgagcgcg ctcgatgccg agaccgaacg gcgcgtcagc
    15961 gagaacctcg cgcgactcaa ctgcacgcgc atcgtgctcg ctcaccggct ctcgacgatc
    16021 atcgccgcag acatgatcat cgtcctcgac cgaggagaaa tcgtcgaggc gggcactcat
    16081 cacgagctgc tggcacgtgg tcagcactat cgtaatctcg tggcagggca gctccgtcag
    16141 gagcaaggcc aggaggtggc gtcatgagag cgctgtggct gatcacattg ctgtgcgctt
    16201 gtcatggcga tacgcgcgag cgagtcgacg agaaggcagc accggaaccg ttgtcggtcg
    16261 ggaagacaac gcagaccgac acgccgaagt ccgcgggcta catcggtgtc ctgacgccga
    16321 aggatgtcgt cgaggtcacc gcgccgttca cgaccaacgt caaacaattt caggtcaagc
    16381 tcggcgagca ggtggaaaag gggactgagc tcgcgctcct cgatcgccaa ccgctcgaag
    16441 agcagcttgc cgtcgcgaaa gccgagctaa aaggcagcga ggcggaggtc ggcgccgcca
    16501 agacgacggc aggcgcagcc gctgcagccc tgcgccgcga gaaagcgggc cagcgcgccg
    16561 gcatcgcgtc gaaagccgat gtctcgaatg cggcatacaa ggccagcgag gcgagctctc
    16621 ttgtctccaa ggccgtcgcg gcggtcgagc agcagaaggc gcgaatcaag gcgctcgaga
    16681 gcaagctcac ccagatgacg ctgaccgcgc cgatcgcggg ccgggtatcc cttcgctacg
    16741 tcgaacctgg cggacgcgtc gaagaaggca aaccgatcgt gcgcgtgatc tcgagcgacg
    16801 agctattcgt gaagttcgcg atcccgagcg agcaggccgg acgcattagc ccgggagacg
    16861 tcgtcgatgt cgtgatcgag ggacgcgacg acaagcttgc cgcgaaggtg aagaccgtcg
    16921 cccccgagct cgatcctgtc gcgcagatga tcgtcgccga ggcggagctc accggtacgg
    16981 tgcccgcgaa gctgcagtcc ggcatggtgt gccggatcgt cgtcgccgac ggagcgaagc
    17041 acgcgaactg atgatgccgc tgctcaccga ctcggccgcg gcacgtgcac gcgatgcggt
    17101 gatcgcgatc gacgcggcga ttcgcgacga cgagagcggc aacgatgggt acgccgcgct
    17161 gttctcggcg tacgtcgcga ccctcgtgcc cgacggcgat gcgcaacaag agcgcatgct
    17221 cgataggctc gtcgaacgcc tcggccagcc gtacgcgcgc gcagggttat tcggtgggct
    17281 cgccggcgat ggctgggtgc tgtgtcacat cggcgacgag tgcgagccgc tgctcgtgca
    17341 gatcgatcgc cagctacacg cggtgctgcc cacgctcgcc gacttcgatc tcatcagcgg
    17401 ctcggcgggc atcgcgatct atttcgccga gcggcttcac cagggcgcag aagctcgcga
    17461 cgcgctggcc accacgtttg ctcatctcgc ctcgggctgg cgcgcagcac ccggcgatga
    17521 ctgcggcctc gcgcacggta cgcccggcgt gatcgcggca gtgacggtcg cggcctcgcg
    17581 cgggatcgtc gacgctgtcg cactgcgcga tgaagcgctc gcttggctgt cgcgcgcgcg
    17641 gctcgcggat ggcgcgtatc cgacgaagct cggtggtcgc cctgcgcgca cggcgtggtg
    17701 ctatggcgac ccgggcatcg tcgctgggct gtggaatgcc ggtg
//
LOCUS       DATLBZ010000578        10421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10082740, whole genome shotgun sequence.
ACCESSION   DATLBZ010000578 DATLBZ010000000
VERSION     DATLBZ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10082740"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VMZ53_28640"
     CDS             complement(<1..352)
                     /locus_tag="VMZ53_28640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32516.1"
                     /translation="MRFLALSALIACAPVPAELAPDIAPDGRPLREVAAPHDRRIGTF
                     LWFATTPDAAFQRALAELDMYIVPVFFRQVQPERGTWDFTLADGVADAAPPGTVLYVP
                     GLITNDLIPDWLASG"
     gene            complement(397..1674)
                     /locus_tag="VMZ53_28645"
     CDS             complement(397..1674)
                     /locus_tag="VMZ53_28645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32517.1"
                     /translation="MRLFGALLVFGVLAACKGAERPRVPAAGSSTIDVSRSGFSLRAV
                     AGDGARTFVGLSTATQSVVEARRGTTVEWRADVPGVVAALALVGPNIIVGTQVSEGMR
                     GDPGAALQATGASGKTTWRVAIDSTEWSLITSMAALDDDILVGGSFGGTLRIADKVVS
                     SAGGSDGFVARISAKGELVWLVRLGGGGADAVQGVATSGSRIAIAGTFTAGADLLGEP
                     LAAYDERLPYADAFAAELDDKGTRRWSKTFGGKGDESVAGVAIDARGNVVVAGNGREV
                     IYVGSQRLVTGGDSDGVVVWYGATGEQGPAILVGGLDYDGLRAIAAVDDRVVVGGFFA
                     GTLTLGDRTLVADGKSGGGDDAFFAALDPRGLVERSWHVGGEGREDIVALSHVSGGFI
                     AAVAHTGSAKLDDATLPAPADPMSGAAIAVRGL"
     gene            complement(1717..1953)
                     /gene="rpmB"
                     /locus_tag="VMZ53_28650"
     CDS             complement(1717..1953)
                     /gene="rpmB"
                     /locus_tag="VMZ53_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HUS32518.1"
                     /translation="MSRVCQVTGKKPLVGSNVSHSNRHTKRRFEINLMNKRFWLEDEK
                     RWISLRVSARGMRLIDKLGLPAVLAQMRARGESV"
     gene            complement(1965..2138)
                     /gene="rpmG"
                     /locus_tag="VMZ53_28655"
     CDS             complement(1965..2138)
                     /gene="rpmG"
                     /locus_tag="VMZ53_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HUS32519.1"
                     /translation="MAKKGKSGRELIRLVSTADTGFFYTTSKNRKRTPDKLVLKKYDP
                     VVRKHVEFKESKI"
     gene            complement(2228..4102)
                     /locus_tag="VMZ53_28660"
     CDS             complement(2228..4102)
                     /locus_tag="VMZ53_28660"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HUS32520.1"
                     /translation="MPSLVFLAGPIAGRRYKLGDGEYVIGRRSDCQIFVPDMRVSRQH
                     ARLWKDGEGWTLEDLGSNNGTYINGVRLQSATQLRHADEIMIANNKIRVEAQDHPSES
                     RPLEGSVVTIVDVSGQALIKSREDSGAGRLPLVSSGMISVADQKAVRLLERKLDALTQ
                     ILVATAASDTEETLLDKLSDALLQLFPQAEDVGMLTEDERTGELHVQVQRHRDRMSGP
                     HAPLTNNASIPTPTDLVRLPTDTGDKKKTYGGELRVPGTIIQHVVSDKRGVLLGEGND
                     EADEAVGTRMGAPLIYHGSHYGVVYVESKTQSFRQEDVDLLQAIATQAGMAMHATRVA
                     AQLVRRERLERDLRVARQIQRSLLPANVPQVVGLDFAVHYEPAYQIGGDFYDFIWHDP
                     AHLGLAVGDVAGKAISAALYMARLTSELRSRAAIARTPARLLRRVNQEIAQLGDDGMF
                     ATLVYCIYDLESRSLVFTNAGHCVPLLRRGDRVFPLQAERAHTPPLGVTPELEAGEAR
                     VQLHSGDMLIMVSDGILEARDPRGVEYGLSRLSRRIRTARGSAEDVIKAILADIDSHT
                     SEAAQGDDMTIVAMSIDQRRAKRKTTTIPGVLPADQASNPRLSTENGTELDSEDEEDG
                     "
     gene            4200..8831
                     /locus_tag="VMZ53_28665"
     CDS             4200..8831
                     /locus_tag="VMZ53_28665"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUS32521.1"
                     /translation="MKSLGNYIGGAFVPPSGTALVSKNPAADGAVVFETGFTVEAVGA
                     AADAAAAAQPGWAALPQAERVAHLERFKAEIAARADQLADAIVLETGKIRSEAKVEIQ
                     TLINRFDLVKAAMAADLKSGAVAPGEYLRYQPLGVVGVIGPFNFPLHLCHAHVVPALL
                     AGNTVVVKPSDVTPLCGQRYAEAAHAAKLPPGVLNVVAGTGAVGAAMVANQHLRGLCF
                     TGSWPVGHRILEASTNRPELLVALEMGGKNSCVVLEDCALRQAVHEVAVGGYLSAGQR
                     CTGTERVLVHRKIADRFIDALARVVRELKFGNPEDASVFAGPVVNEAALQKVEGAIAA
                     ARRVGTGGAEPIVEGKKLPGGYYRSASLHRLPDGKHAIEGYTDIEVFGPDLSIEVIDS
                     DDEAVSIINSSPYGFVNAVFTASRERFEAFAAKTKSGMLNRNRSTNLASPKLPFHGVG
                     KSGNYRPAGAWAHRNVTAPLSILENVLGAVTPHPHISPLLPSVDLDRLEAQHAAEEAE
                     EATRTLVDMPRPMALHRPAGGQLPESAALLARLYAGDRVPKEKKPPVFDHLRSAGPWM
                     VSIDKQPLSVLDGMSQTATVVGGFSEDPVVAAFTEGEFADTLVINDDTSVRETAAAID
                     YANALRQLVPGLPHVTFVASGAEANEKAMALCRINSPRPNATKILAFEGSFHGRTYLA
                     LHATHSPTKRKQFELAGYECTFAPFPVWDTPGDEPTAPSGFYAACANGAIDDLVERFG
                     DAKDDPLLAAEVASLAAVHHAIKTGEYNAVFIEPMQSEGGDRYATERFFRALRLLTRH
                     HQTFLVFDEVQVGFGLGGPFAWHSKFRLLSSRGQQDYPDAVTFAKRAQVGVVMSRFSD
                     PEHASAHNASLVRGRIHADMVSMSHNAERIEKLVAPRLAQIARAYPHLVQHPRAAGYA
                     FAFDLPTPALLEAFINQRFWRGAIVFAAGTKTARYRLSDSFLAREIDMLFEAIRRSLS
                     WLDAHPDRKPPEWEDQPPGAPDKVPPSEVRYRLVPHAEVMEHLPAILDIEYQVYEPAR
                     RTPPAEIRAAIEDIDGSLIVAEAPSPAAGTWQLVGFAIGGPLEQSKDVEGCDDDPMLG
                     KHNTMYSVSITVAPSYQSSGIGRKLKEQQLRDAASRKKPDGAFRYRYVTGRNRVGRTA
                     QMTHLNRVFGAHVVSVLTGQYEDPEGQAIYYRIPLGAFAPDPIVKQEVVRSWTARGDA
                     QIELDLASGLTKPFANGPASLRDAETKGLLYGPAVNKLTLMNYVSPGAVRALEWIGAL
                     IPELPHMYLTSSRDETVDKTLRMFKLARKEATIAIALEGGYYGHTVASCRSLSDPSTH
                     RGGPGHFAWPRVPHPARAGTDETIAAIRSVVAAAGGPSKVLCFVYEIVQERTGAVLPK
                     DFVAALAALRNELDVPLVAVETTTHSYRSGLGAFASPALGLIPDALVWWGGGQTGYVH
                     TSTRWFVPGPLTLVSTWDGDELSLVRQHHQLRAARHLDIAAGARALEAALPGSQGLGA
                     YRVLDASKDVLEALADDHIAVRQFPNGKLGIIPALDQLDVAAQKLREAL"
     gene            8840..9976
                     /locus_tag="VMZ53_28670"
     CDS             8840..9976
                     /locus_tag="VMZ53_28670"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HUS32522.1"
                     /translation="MLELPAGKSPREWGRIHGESFRGEIHSLSAIRTYLCTKVGAFTT
                     HEQILAAAKAHLPVLERYSHPLYEELLGIAEGAIISPEEIVVANHYTDLRDLDPDPER
                     WRMAPTGDDPDMTNGGRTQPVSTQLDPASGGEGCSMIWSQTPTGRILAQTWDMHATAI
                     PYVMVLGIPESEDGPAARLLTVTGCLGMAGMNASRVAIAINNLYSTDATLGVVWPAMV
                     RRALHEDTAADARDVIARSPIGSGHHYFVADKTSAFSLEASGTRRRLVFAGERSQFCH
                     TNHCLDADVAERSKISPTSTTHERMQWLEHDLARQGLQDLDDAWKRLGSEDGWPKSIC
                     TNMATPEAPHGTATCGAIAMNMDTGELWAQQGLIHNVAPEKWQL"
     gene            10110..>10421
                     /locus_tag="VMZ53_28675"
     CDS             10110..>10421
                     /locus_tag="VMZ53_28675"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUS32523.1"
                     /translation="MSEYPFFFTWTAQNAAKPVELAGGEGAWFTLKDGSRWLDLGALA
                     YQVNVGHGNKRIVEAIKRQADELCLSTPNAVFPAKVELAERLLKLAGPAFSKVFFTLG
                     GA"
BASE COUNT         1728 a         3576 c         3432 g         1685 t
ORIGIN      
        1 tgccggacgc tagccagtcc gggatcagat cgttcgtgat gaggcccggc acgtacagca
       61 ccgtccccgg tggtgcggcg tccgcaacac cgtcggcgag cgtgaagtcc cacgtgccgc
      121 gctcgggctg cacctggcgg aagaagacgg gaacgatgta catgtcgagc tcggcgagcg
      181 cacgctggaa tgcggcatcg ggcgtcgttg cgaaccacag gaacgtgccg attcgtcgat
      241 cgtgcggagc agcgacctcg cgcaacggcc ggccatcggg cgctatgtct ggtgcgagct
      301 ccgccggcac cggcgcgcac gcgatgagag cggacagcgc caggaaccgc acgctcgggt
      361 tgtaacgcgc tgctgcccgc atgtcattcg ccgtcgctac aggccgcgca ccgcgatcgc
      421 ggcaccgctc atcggatcgg caggcgccgg cagtgtcgcg tcatcgagct tcgcgctgcc
      481 ggtatgcgcg acagccgcta taaaaccgcc gctcacgtga gagagcgcga cgatgtcctc
      541 gcggccttca ccgccgacat gccacgaacg ctcgacgagg cctcgcggat cgagcgcggc
      601 gaagaacgcg tcatcaccgc cgccgctctt gccgtccgcg acgagcgtgc gatcgccgag
      661 cgtcaacgtg cccgcgaaga atccgccgac gaccacgcga tcgtcgactg ctgcgatcgc
      721 gcgtaggccg tcgtagtcga ggccaccgac gaggatcgcg ggaccttgct cgccggttgc
      781 gccgtaccac acgacgacgc catcgctatc gccgccggtg acgagacgct ggctgccgac
      841 gtagatcacc tcgcggccgt tgcccgcaac gacgacattg ccgcgcgcat cgatcgcgac
      901 gcctgcgacc gactcgtcgc ctttgccgcc gaacgtcttg ctccagcggc gcgtaccctt
      961 gtcgtcgagc tcggcagcga acgcgtcggc gtagggcagg cgctcgtcgt acgcggcgag
     1021 gggctcgccg agaagatcgg cgccggctgt gaacgtgcct gcgatcgcga tgcgcgagcc
     1081 ggatgtggcg acgccctgca cggcatctgc gccaccgcca ccgaggcgca cgagccagac
     1141 gagctcgccc ttcgccgaga tgcgcgcgac gaagccgtcg ctgccgcccg cgctcgagac
     1201 gaccttgtct gcgatgcgca gcgtgccgcc gaacgatccg ccgacgagga tgtcgtcgtc
     1261 gagcgcggcc atgctcgtga tgagcgacca ctcggtcgaa tcgatcgcga cgcgccacgt
     1321 cgtcttgccg cttgcaccgg tcgcttggag agctgcacct gggtcgccgc gcatgccttc
     1381 gctgacctgc gttccgacga tgatgttcgg accgacaagt gcgagcgcag cgacgacgcc
     1441 gggaacatcg gcgcgccatt cgaccgtcgt tccgcgtcgc gcttcgacga ccgactgcgt
     1501 cgcggtgctg aggccgacga acgtgcgtgc gccatcaccg gcgaccgcgc gcagcgaaaa
     1561 gcccgagcga ctcacgtcga tcgtcgatga accggcagcg ggcacgcgcg ggcgctccgc
     1621 gcctttgcag gcagcgagca ccccgaaaac gagcaatgcg ccgaagaggc gcatggcgaa
     1681 gctacgtgca ccgaagcggc gcacgcgatg cggaagctag accgactcgc cgcgggcgcg
     1741 catctgcgcg agcaccgccg gcaggcccag cttgtcgatc aggcgcatgc cgcgcgccga
     1801 gacgcgaagc gagatccaac gcttctcgtc ttcgagccag aagcgcttgt tcatgaggtt
     1861 gatctcgaag cgacgcttgg tgtggcggtt cgagtggctg acgttggagc cgacgagcgg
     1921 cttctttccg gtgacttggc aaacgcgtga catggcccgt ctccttagat cttcgactct
     1981 ttgaactcga cgtgcttgcg aacgaccggg tcgtacttct tcagcacgag cttgtccggg
     2041 gtgcgcttgc ggtttttcga ggtcgtgtag aagaagccgg tatctgccgt cgaaaccaga
     2101 cggatcagct cgcggcctga cttacctttt ttcgccatga cacttcgtcc taaagcaccc
     2161 aggaagggtt ccgggggctg ggacttatgg catggactcc cgcgatttgg caaggctcca
     2221 aagcgcgcta gccgtcctct tcatcctcgc tgtccagctc cgtgccattc tcggtcgaga
     2281 ggcgcgggtt cgaggcttgg tcggccggca aaaccccagg gattgtcgtg gttttacgct
     2341 tcgcccggcg ctggtcgatc gacatcgcca cgatcgtcat gtcgtcgccc tgggcggcct
     2401 cgctggtgtg gctatcgatg tctgcgagga tcgctttgat cacgtcttcg gcgctcccgc
     2461 gggcggtccg gatccggcgg gacaggcgcg atagcccgta ctcgaccccg cgcgggtcgc
     2521 gcgcttcgag gatgccgtcg ctcaccatga tgagcatgtc gccggagtgg agctgcacgc
     2581 gggcctcacc ggcttcgagc tccggcgtga cgccgagtgg cggcgtgtgc gcgcgctccg
     2641 cctgcaacgg gaacacgcga tcgccgcggc gcaaaagcgg cacgcagtgg ccggcattcg
     2701 tgaacacgag gctgcgcgac tcgagatcgt agatgcagta gacgagcgtc gcgaacatgc
     2761 cgtcgtcgcc gagctgcgcg atctcttgat tgacgcgacg gagcaggcga gccggcgtgc
     2821 gtgcgatcgc agcgcgagac cgcagctcgc tcgtgagccg cgccatgtag agcgccgcgc
     2881 tgatcgcttt accggcgacg tcgccgaccg cgaggccgag atgtgcgggg tcgtgccaga
     2941 tgaagtcgta gaagtcgccg ccgatctgat acgcgggctc gtagtgcacg gcgaagtcga
     3001 ggccgacgac ctgcggcacg ttcgccggaa gcagagagcg ctggatttgc cgcgcgacgc
     3061 gaagatcacg ctcgagcctc tcgcgacgaa cgagctgcgc agcgacgcgc gtcgcgtgca
     3121 tcgccatgcc ggcttgcgtc gcgatcgcct gcaatagatc gacgtcttct tgccggaacg
     3181 actgcgtctt gctctcgacg taaacgacgc cgtagtgaga gccgtgatag atgagcggcg
     3241 cgcccatgcg ggtaccgacg gcttcgtcgg cctcgtcgtt gccctcgccg aggagaacgc
     3301 cgcgcttgtc gctgacgacg tgctggatga tcgtgcccgg cacgcggagc tcgccgccgt
     3361 acgtcttctt cttgtcgccc gtatccgtcg gcaagcgcac caagtcggtg ggcgttggta
     3421 tgctcgcatt gttcgtcaac ggcgcgtgtg gacctgacat ccggtcgcgg tgcctctgca
     3481 cctggacatg cagctcgccc gtgcgctcgt cctcggtgag catgccgacg tcttcggctt
     3541 gcgggaacag ctggagcagc gcgtccgaca gcttgtcgag cagcgtctct tcagtgtccg
     3601 atgcggcggt cgccacgagg atctgcgtga gcgcgtcgag cttgcgctcg agcaggcgta
     3661 cggccttttg atccgcgacc gagatcatgc ccgagctgac gagcggcagg cggcccgcac
     3721 cggagtcctc gcggctcttg atgagcgcct ggcccgagac gtcgacgatc gtgacgacac
     3781 tgccctcgag cggcctcgac tcgctcgggt gatcctgcgc ctcgacgcgg atcttgttgt
     3841 tcgcgatcat gatctcgtcg gcgtggcgca gctgcgtcgc cgattgcagc cgcacgccgt
     3901 tgatgtacgt gccgttgttg ctgccgaggt cctcgagcgt ccaaccctcg ccgtctttcc
     3961 acagccgcgc gtgctgacga gagacgcgca tatcggggac gaagatctgg caatccgacc
     4021 ggcgcccgat cacgtattcg ccatcgccga gcttgtagcg acggcccgcg atcggtccgg
     4081 ccaaaaagac cagggagggc atctaggccc gcttgatagc acggtttttt gtccgtgacc
     4141 cagacgagac gcgcgccctg tacctgcatc tttgggcctc gatatggtct atttgcgcgg
     4201 tgaagtccct cggcaattac atcggtggcg cgttcgtccc gccatcgggt accgccctcg
     4261 tgtcgaagaa ccccgccgcc gacggtgcgg tcgtgttcga gacgggcttc acagtcgagg
     4321 cggtcggggc ggcggcggac gctgcggcgg ctgcgcaacc aggatgggct gcacttccgc
     4381 aggccgaacg cgtcgcgcac ctcgagcggt tcaaggccga aatcgccgcg cgcgccgacc
     4441 agcttgccga cgcgatcgtg ctcgagacgg gcaagatccg cagcgaagcg aaggtcgaga
     4501 tccagacgct catcaatcgc ttcgatctgg tgaaggccgc gatggccgca gatctaaaga
     4561 gcggtgcggt cgcgcccggt gagtatctgc ggtaccagcc gcttggcgtc gtcggcgtga
     4621 tcggtccgtt caacttcccg ctgcacctgt gccacgcgca cgtcgtgccg gcgctgctcg
     4681 cggggaacac cgtcgtcgtg aagccaagcg acgtcacgcc gttgtgcggt cagcgctacg
     4741 ccgaagcggc gcacgccgcg aagctgccgc cgggtgtgct caacgtcgtc gcggggacgg
     4801 gcgcggtcgg cgcggcgatg gtcgcgaacc agcatctgcg cgggctgtgc ttcaccggaa
     4861 gctggccggt cggtcaccgc atcctcgaag cgtcgacgaa tcggcccgag ctgctggtcg
     4921 cgctcgagat gggcggcaag aactcgtgcg tcgtgctcga agactgcgcg ctgcgccagg
     4981 ccgtgcacga ggtagccgtc ggtggctatc tctcagcggg ccagcgctgc accggaaccg
     5041 agcgcgtgct cgtccatcgc aagatcgcgg accgcttcat cgatgcgctc gcgcgcgtgg
     5101 ttcgcgagct gaagttcggc aaccccgagg acgcgagcgt gttcgcgggg ccggtcgtca
     5161 acgaggcagc gctgcagaag gtcgagggcg cgattgcggc ggcccgaaga gttgggacgg
     5221 gcggggcaga gccgatcgtc gagggtaaga agctgcctgg tggctactac cgctcggcgt
     5281 cgctgcatcg attgcccgac ggcaagcacg cgatcgaggg ctacaccgac atcgaagtgt
     5341 tcgggcctga cctctcgatc gaggtgatcg attccgacga cgaagcggtg agcatcatca
     5401 acagctcgcc atacggattc gtcaacgcag tgttcactgc atcgcgcgag cggttcgaag
     5461 cattcgccgc gaagacgaag tcgggcatgc tcaaccgcaa ccgctcgacg aacctcgcga
     5521 gccccaagct cccgttccac ggcgtcggca agagcggcaa ctatcgtccc gcaggcgcgt
     5581 gggcgcatcg caacgtcacc gcgccgctgt cgatcctcga gaacgtgctc ggtgccgtca
     5641 cgccgcatcc gcacatctcg ccgctgctgc ctagcgtcga tctcgatcgg ctcgaagcgc
     5701 agcacgccgc ggaagaagcg gaagaggcga cgcgcacgct cgtcgatatg ccgcgaccga
     5761 tggcgctgca tcgccccgct ggtggccagc tccccgagag tgccgcattg ctcgcgcgcc
     5821 tctatgccgg tgaccgcgtg ccgaaagaga aaaagccgcc ggtcttcgat cacctgcgct
     5881 cggcaggccc gtggatggtc tcgatcgaca agcagccgct cagcgtgctc gacggcatga
     5941 gccagacggc gaccgtcgtc ggaggattct ccgaggatcc cgtcgtcgcc gcgttcaccg
     6001 aaggcgagtt cgcagacacg ctcgtgatca acgacgacac gtccgtgcgc gagacggcgg
     6061 ccgccatcga ctacgcgaat gcgctgcgcc agctcgtgcc gggtctgccg cacgtgacgt
     6121 tcgtggcgtc cggcgccgag gccaacgaga aggcgatggc gctgtgccgc atcaactcgc
     6181 cgcggccgaa tgcgacgaag attctcgcgt tcgaaggcag cttccacggg cgcacatatc
     6241 tcgcgctgca cgcgacgcac tcgccgacga agcgcaagca gttcgagctc gccggctacg
     6301 aatgcacgtt cgcgccgttc ccggtctggg acacgcctgg cgacgagccg acggcaccga
     6361 gcggcttcta cgccgcgtgc gcgaacggcg cgatcgatga tctcgtcgag cggttcggcg
     6421 atgcgaagga tgacccgctg ctcgccgccg aggtcgcctc cctcgcggcc gtgcatcacg
     6481 cgatcaagac gggcgagtac aacgccgtct tcatcgagcc gatgcaatcg gaaggcggcg
     6541 accgctacgc gacggagcgc ttcttccgcg cgctgcgttt gctcacgcgc catcaccaga
     6601 cgttcctcgt gttcgacgaa gtgcaggtcg gcttcgggct cggcggcccg tttgcgtggc
     6661 acagcaagtt ccggctgctg tcgtcgcgcg gccagcagga ctatccggac gccgtgacgt
     6721 tcgcgaagcg cgcgcaggtt ggcgtcgtga tgtcgcggtt ctcggatccc gagcatgcga
     6781 gcgctcacaa tgcctcgctc gtgcgcggcc gcatccacgc ggacatggtc tcgatgagcc
     6841 acaacgccga gcgcatcgag aagctcgtcg ccccgcggct cgcgcagatc gcgcgcgcgt
     6901 atccgcatct cgtccagcat ccgcgcgctg cgggctatgc gttcgcgttc gacttgccga
     6961 cgcccgcgct actcgaggcc ttcatcaacc agcggttctg gcgcggcgcg atcgtgtttg
     7021 ccgcgggcac gaagaccgca cgctatcgcc tcagcgattc gttcctcgcg cgcgagatcg
     7081 acatgctgtt cgaagcgatc cgccgctcgc tgtcgtggct cgacgcgcat cccgatcgca
     7141 agccgcccga gtgggaagac cagccgccgg gcgcaccaga caaagtcccg ccgtcggagg
     7201 ttcgctaccg cctcgtgccg cacgcagagg tcatggagca tctgccggcg atcctcgaca
     7261 tcgagtacca agtctacgag cccgcgcgcc gcacgccgcc cgccgaaatc cgcgccgcga
     7321 tcgaggacat cgatggctcg ctgatcgttg ccgaagcgcc gtcgccggca gcgggcacct
     7381 ggcagctcgt cggcttcgcg atcggtggcc cgctcgagca gagcaaagac gtcgaaggct
     7441 gcgacgacga tccgatgctc ggcaagcaca acacgatgta ctcggtctcg atcacggtcg
     7501 cgccgagcta ccagtcgagc ggcatcggtc gaaagctcaa ggagcaacag ctccgggacg
     7561 cggcgagccg caagaagccc gacggcgcgt tccggtatcg gtatgtcacc ggccgcaacc
     7621 gcgtcggccg caccgcgcag atgacgcacc tcaatcgcgt gttcggcgcg cacgtcgtgt
     7681 cggtcctcac cggccaatac gaagatccgg aagggcaggc gatctactac cggatcccgc
     7741 tcggtgcctt cgcgcccgat ccgatcgtga agcaagaggt cgtgcgctcg tggaccgcac
     7801 gcggcgatgc gcagatcgag ctcgatctcg cgtcgggcct gacgaagccg ttcgcgaacg
     7861 ggccagcgtc gctgcgcgat gccgagacga agggcctcct ctacggtccc gccgtcaaca
     7921 agctcacgct gatgaactac gtctcgccgg gcgcggtccg cgcgctcgag tggatcggtg
     7981 cgctcattcc cgagctgccg cacatgtatc tgacgagcag ccgcgacgag accgtcgaca
     8041 agacgctgcg catgttcaag ctcgcgcgca aagaagcgac gatcgcgatc gcgctcgaag
     8101 gcggctacta cggccacacc gtggcgtcgt gccgctcgct ctccgatccg tcgacgcatc
     8161 gtggtggccc cggtcacttc gcgtggccgc gcgttcctca ccccgcgcgt gcgggtaccg
     8221 acgaaacgat cgcggcgatt cgctcggtgg tcgctgcggc tggcggaccg tcgaaggtgc
     8281 tctgcttcgt gtacgagatc gtgcaagagc gcacgggcgc cgtcctcccg aaggacttcg
     8341 tcgccgcgct cgccgcgctt cgcaatgagc tcgacgtccc actcgtcgcg gtcgagacca
     8401 cgacgcactc gtatcgctcg ggcctcggcg cgttcgcatc gcccgcgctc ggcctcatcc
     8461 ccgacgcact cgtgtggtgg ggcggcggtc aaacgggcta cgtgcacacg tcgacgcgct
     8521 ggttcgtgcc gggcccgctg acgctcgtct cgacgtggga cggtgacgag ctctcgctcg
     8581 tgcgccagca ccaccagctg cgcgccgctc gccacctcga catcgccgcg ggcgcgcgcg
     8641 ctctcgaagc cgcgctgccg ggctcgcagg gtctcggcgc gtaccgcgtg ctcgacgcga
     8701 gcaaagacgt cctcgaagcg ctcgccgacg atcacatcgc cgtgcgccaa ttccccaacg
     8761 gcaagctcgg catcatcccc gcgctcgacc agctcgacgt cgctgcgcaa aaactgcgag
     8821 aggccctctg acgatgcgcg tgctcgagct gccagccggt aaatccccgc gcgagtgggg
     8881 ccgcatccac ggtgaatcgt ttcgcggcga gatccactcg ttgtccgcga tccggacgta
     8941 cctttgcacg aaagtcggcg cattcacgac ccacgagcag atcctcgccg cggcgaaagc
     9001 gcacctgcca gtcctcgagc gctacagcca tccgctctac gaagagctgc ttggcatcgc
     9061 cgaaggcgcg atcatctcgc ccgaagagat cgtcgtcgcg aaccactaca ccgacctgcg
     9121 cgacctcgac cccgatccgg aacgctggcg catggcgccg acgggcgacg accccgacat
     9181 gacgaacggc ggtcgcacgc aaccggtctc cacgcagctc gatcccgcat caggcggcga
     9241 aggctgctcg atgatctggt cgcagacgcc gacgggccgc atcctcgctc agacctggga
     9301 catgcacgcg accgccatcc cgtacgtgat ggtgctcggc atccccgagt ccgaagatgg
     9361 cccggccgcc cgcctgctaa cggtcacagg ctgcctcggc atggccggca tgaacgcctc
     9421 gcgcgtcgcg atcgcgatca acaatctcta ctcgaccgac gccacgctcg gtgtcgtgtg
     9481 gcccgcaatg gtccgccgcg cgctgcacga agacacggcc gccgatgccc gcgacgtcat
     9541 cgcccgctcg ccgatcggct cgggccacca ctacttcgtc gccgacaaga cctccgcctt
     9601 ctcgctcgaa gccagcggca cgcgccgccg cctcgtcttt gccggtgagc gatcgcagtt
     9661 ctgccatacg aaccactgcc tcgacgcgga cgtcgcggaa cgcagcaaga tctcgccgac
     9721 gtcgacgacg cacgagcgca tgcagtggct cgagcacgac ctcgcgcggc aaggcctcca
     9781 ggacctcgac gacgcgtgga agcgcctcgg ctccgaagac ggctggccga aatcgatctg
     9841 cacgaacatg gcgacccccg aagccccgca cggcaccgcc acctgcggcg cgatcgcgat
     9901 gaacatggac acgggcgagc tctgggccca acaaggcctc atccacaacg tcgcgccgga
     9961 gaagtggcag ctgtgagcgg catgaacacg cagcggcgca ctacccgcgc atccacgccg
    10021 agtggtggtt caactcgccg attaattcag tccgacctaa tttctggtca gaccacaact
    10081 tcagtccgac ctaatttcct gcggcagcga tgagcgagta cccatttttc ttcacgtgga
    10141 cggcgcagaa cgcggcgaag cccgtcgagc tcgcgggcgg cgagggcgcg tggttcacgc
    10201 tcaaagacgg cagccggtgg ctcgacctcg gcgcgctcgc gtatcaagtg aacgtcggcc
    10261 acgggaacaa gcgcatcgtc gaggcgatca agcggcaggc ggatgagctg tgtctgtcga
    10321 cgccgaatgc ggtgtttccg gcgaaggtcg agctcgccga gcgtctcctg aagctcgcag
    10381 gaccggcgtt cagcaaagtc ttcttcacgc tcggcggcgc c
//
LOCUS       DATLBZ010000579         3139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8617306, whole genome shotgun sequence.
ACCESSION   DATLBZ010000579 DATLBZ010000000
VERSION     DATLBZ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8617306"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="uppS"
                     /locus_tag="VMZ53_28680"
     CDS             <1..634
                     /gene="uppS"
                     /locus_tag="VMZ53_28680"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HUS32524.1"
                     /translation="AESVRDITRASRELGLEALTLYAFSSQNWARPADEVAGLMQLLR
                     DFLIDERSEILDNQIRLEALGDIERLPALVREPLDELRAASSGNSKMVLTLALSYGGR
                     ESIARAAQKAAADVAAGIIRADQLDCDRLGSYLPTSHLPELDLLIRTSGEQRVSNFML
                     WEVAYAELMFVDIMWPDFRREHLYQCLDAYKTRERRFGLTTAQLAESSDV"
     gene            651..1568
                     /locus_tag="VMZ53_28685"
     CDS             651..1568
                     /locus_tag="VMZ53_28685"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HUS32525.1"
                     /translation="MALGNLAQRFLVAIIAVPILLLLLHYHRHEPTWALVFVASLIAM
                     HEFFAMTLPKQDRGAALVMGAVAAAAFYWLDFHTGSYFEGHSDNARRMAGFIYAGNAI
                     CVVLAVIVPGLYYLFRFRDISSVAGRYAATVAGIIYAGFLTTFLAFLKRIDPNHGGDT
                     VVLVLIVAWVADTGGYFAGRFLGKAKLYEAVSPKKTWAGLWGGIAGAVVGVIALKLFM
                     EAFGDAHRVKWLTWFDVFAISIPGCLLGQMGDLAESLIKRSTGVKDSGTLLPGHGGIL
                     DRIDAVLFIAPYVYVYLIVKYPALYAAGL"
     gene            1777..2715
                     /locus_tag="VMZ53_28690"
     CDS             1777..2715
                     /locus_tag="VMZ53_28690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HUS32526.1"
                     /translation="MRARTIAFCAAACAFSACGPSAPNHNGGDDDGSPDANNGGGGGG
                     GGGTVDAAGCSKMDLLFVIDNSGSMGQEQANLIANFPTFISVLDASGLDYRVAVTTTG
                     RNYTYNMATPIGNIPESQTGGDNGTMLKPASCNMTKRWIDKNDPNPSMTFSCLANVGT
                     GGPSDEMPLGAVRDAFEDRMADGTNMGFRRSDALLGVVILTDEEDCSYEHSVNLSFSE
                     SLCENQMEPVANYKSFLDTYAGGPTRWATAIIAGKGPGACSSSFGDAQEATRLVQFKG
                     LVGTNAIMSSICDGDLSLGLQDALNLFHSACDAIIL"
     gene            complement(2705..>3139)
                     /locus_tag="VMZ53_28695"
     CDS             complement(2705..>3139)
                     /locus_tag="VMZ53_28695"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS32527.1"
                     /translation="AAEDRGDSELAEHLFRQAIDLEPNMAAALTNLGNLVYRQGEVEE
                     ARRLYERALEHDPNQAEARYNLANALEDLGEIEAAVTELRKVCAQAPEFADAHYNLGL
                     LLAQLGGAAQARQHLERYLELDAQSDWATHARQYLETAVATG"
BASE COUNT          514 a         1005 c         1027 g          593 t
ORIGIN      
        1 cgcagagagc gttcgagata tcacacgcgc gtcgcgtgaa ttgggtctcg aagcgctgac
       61 gctctacgcg ttctcgtcgc agaactgggc tcggcctgcc gacgaagtcg cgggtttgat
      121 gcagcttctt cgcgacttcc tcatcgacga acgcagcgag attctcgaca accaaattcg
      181 cctcgaagcg ctcggcgata tcgaacgtct tccggcgctc gtgcgcgagc ccctcgatga
      241 gctacgcgca gcgtcgtctg gcaattccaa gatggtgctg acgctcgcgc tgtcgtacgg
      301 cgggcgcgag tcgatcgcac gcgcggcaca aaaagctgcg gccgatgtcg cggcgggaat
      361 aattcgcgcc gaccagctcg actgcgatcg cctcggctcg tatctgccga cctcgcatct
      421 acccgagctc gatctgctta ttcgcacgtc gggcgagcag cgcgtctcga acttcatgct
      481 gtgggaagtc gcgtacgccg agctgatgtt cgtcgacatc atgtggccgg acttccggcg
      541 cgagcacctc taccagtgcc tcgatgcata caagacgcgc gaaagacgct tcggacttac
      601 gaccgctcag cttgccgaat cctctgacgt gtagttagct ccaaggcctc ttggctctcg
      661 gcaatctcgc gcagcggttc ctcgtcgcga tcatcgcagt cccgatcctg ctgctgctgc
      721 tccactacca tcggcacgag ccgacgtggg ctctcgtgtt cgtcgcgtcg ctgatcgcga
      781 tgcacgagtt cttcgcgatg acgctgccga aacaagatcg cggcgcagca ctcgtgatgg
      841 gcgccgtcgc agcggccgcg ttctactggc tcgacttcca caccggctcg tacttcgagg
      901 gccacagcga caacgcgcgg cgcatggcgg gcttcatcta cgcgggcaac gcgatctgcg
      961 tcgtgctcgc cgtgatcgtg ccgggcctct actacctgtt tcgcttccgc gacatctcgt
     1021 cggtagcggg tcgctatgcg gccaccgtgg ccggcatcat ctacgcgggc tttctcacga
     1081 cgttcctcgc gttcctgaag cgcatcgatc cgaatcatgg aggcgacacc gtcgtgctcg
     1141 tgctcatcgt cgcgtgggtc gcggacaccg gtggctactt cgcgggccgc ttcctcggca
     1201 aggcgaagct ctacgaagcc gttagcccga agaagacgtg ggccggctta tggggcggca
     1261 tcgcaggcgc ggtcgtcggc gtgatagcgc tcaagctctt catggaagcc ttcggcgacg
     1321 cacatcgcgt caaatggctc acgtggttcg acgtcttcgc gatctcgatt ccaggatgcc
     1381 tcctcggcca gatgggcgac ctcgccgagt cactgatcaa gcgatccacg ggcgtgaagg
     1441 actcgggaac gctgctgccc ggccacggtg gcatcctcga ccgcatcgac gcggtgctgt
     1501 tcatcgcgcc gtacgtctac gtctacttga tcgtgaagta ccccgcgctc tacgcggccg
     1561 ggctctgacc cagccctcga tcgcctcggg tccccgtaga cccagctgcg tcgcccaacc
     1621 gcgcgaaagt gtgcgaccgg ccgcctggca ggggctctgc acgatgggcc cgccgtgact
     1681 tggttcgacc gccaccttga gccgtacgcc gttctcgtcg tcgcgttgat tttgcttttc
     1741 gggacccgct gctgaggatt acagtccgcc gcgatgatga gggccagaac gatcgcgttc
     1801 tgtgctgcgg cgtgcgcatt cagtgcgtgc ggtccgtcgg caccaaacca caacggcggt
     1861 gacgatgacg gctcgcccga cgccaacaac ggcggcggtg gcggcggagg aggaggcacg
     1921 gtcgatgcgg cgggctgctc gaagatggat ctgctcttcg tcatcgataa ctcgggatcg
     1981 atggggcagg agcaagcgaa cctgatcgcg aacttcccga cgttcatctc ggtgctcgac
     2041 gcatcgggcc tcgactatcg cgtcgcggtc acgacgacgg gccgcaacta cacgtacaac
     2101 atggcgacgc cgatcgggaa catccccgag agtcagacgg gcggcgacaa cggcacgatg
     2161 ctgaagccgg cgagctgcaa catgacgaag cggtggatcg acaagaacga tccgaatccg
     2221 tcgatgacgt tctcgtgtct cgcaaacgtc ggcaccggcg ggccgtccga cgagatgccg
     2281 ctcggcgcgg tgcgcgacgc gttcgaagat cgcatggccg atggcacgaa catgggcttc
     2341 cgccgcagcg acgcgctgct cggcgtggtc atcctgacgg acgaagagga ctgctcgtac
     2401 gagcattcgg tgaacctcag cttctcggag tcgctctgcg agaatcagat ggagccggtc
     2461 gcgaactaca agtcgttcct cgacacgtac gccggcggac cgacgcgctg ggcgacggcg
     2521 atcatcgcgg gcaagggccc cggcgcgtgc tcgtcgagct tcggcgacgc gcaggaagcg
     2581 acgcgcctcg ttcagttcaa ggggctcgtc gggacgaacg cgatcatgtc gtcgatctgc
     2641 gacggcgatc tatcgctcgg cctgcaggac gcgctgaacc tcttccacag cgcctgcgac
     2701 gcgatcatcc tgtagcgacg gcggtctcga gatactggcg ggcgtgtgtg gcccaatcgc
     2761 tttgcgcgtc gagctcgagg tagcgctcga ggtgttggcg cgcttgggcg gcgccgccga
     2821 gctgcgcgag tagcaggccg aggttgtagt gcgcgtcggc gaactcgggc gcttgcgcgc
     2881 agaccttgcg tagctcggtg acggcggctt cgatctcgcc gaggtcttcg agcgcgttgg
     2941 cgaggttgta gcgagcttcg gcttggttgg gatcgtgctc gagcgcgcgc tcgtacagac
     3001 ggcgagcctc ttcgacttcg ccttggcgat agacgaggtt gccgaggttc gtcagcgcgg
     3061 cggccatgtt cggctcgaga tcgatcgctt ggcggaacag gtgctcggcg agctcggagt
     3121 cgccgcgatc ttcggcggc
//
LOCUS       DATLBZ010000580        12979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3417241, whole genome shotgun sequence.
ACCESSION   DATLBZ010000580 DATLBZ010000000
VERSION     DATLBZ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12979
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3417241"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1708
                     /locus_tag="VMZ53_28700"
     CDS             <1..1708
                     /locus_tag="VMZ53_28700"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HUS32528.1"
                     /translation="AVVVLDGVAGVEPQTETVWRQADRYRVPRIVFVNKLDRVGADYQ
                     RSLDKLRSRFGVRAVAVTWPLYENDVLVGVIDLVDQTEVRWSDDAGFAFSATPVTLSD
                     NLQATRDRLLEAAADEDPEILAAVVSGLAIPPARLRAALRRGTVSGRLVPVLAGSAYK
                     NRGVQPLLDAVVSYLPSPSDREPVKAADGSAERKPSRTESLAALAFKVVFDDHGQLSF
                     VRVYSGVLARGETIVTGRGRKLRIGRLVQLVAAEQVEVDALGAGEIGAVLGAPLPGGE
                     TLSDPAQPIVLEAITAPDPVMRVAIEARTAAERDKLALALGRMVAADPSLRLESDQET
                     GQTLLAGMGQLHLDVAIERLATEHRVHVTAGAPRVAYRTTLTKTVQRELRHIRQRGGP
                     GQYAHVVVEVGPAPRGSGLLFEDKTKGGVVPKSYAKAVEAGVRGAMSEGLLGGHPVVD
                     VKVSLLDGSTHEKDSNEQAFTVAGSLAFKAAAADTAPIKLEPIMLLEVSVPEDEVGNV
                     IGDLGRRRGEVLALDAQGADRVVRAEVPLAETFGYASALGNLTHGRGRFTLEPARYEP
                     VR"
     gene            complement(1748..2116)
                     /locus_tag="VMZ53_28705"
     CDS             complement(1748..2116)
                     /locus_tag="VMZ53_28705"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HUS32529.1"
                     /translation="MQCPICKSALEQAGRTYKCETCDGSWVRAEVMVPLLEQSAATLV
                     SLDWQSSSEDHHRVCPECGAPMQTVKLGTVNLDRHEPHGVWFDSKELAELLSEAKEFR
                     ADLPPHETLMNKLRRLFDRK"
     gene            2156..4378
                     /locus_tag="VMZ53_28710"
     CDS             2156..4378
                     /locus_tag="VMZ53_28710"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32530.1"
                     /translation="MTWRRALVFACTLAGCDRLFAITHVPDPPLDAYQPDAAPPCLVN
                     DIAVGRAHACAIIPSGDVYCWGENGYGQIQPSGPEYVLTPAKIFLPGPAAEIALGRTH
                     TCARVGGDVYCQGQNNSGQLGKGPGDTSPALRKVDGIPPSAQIAAGGAHVCSRSATDG
                     AVWCWGRNRHSETGGTSVLCSGNTACALPGAVPGITGAKTVVAGHTGTCIIDASDQLF
                     CWGRNDYGQLGIGNSIGTATPTLAMVTPPVRAVDMGGKNMCAIDGMHRAFCSGEGELG
                     TLGNGGFQATSTPVQVFTGVPVQISSASFGACAVTDDGMLSCWGRGETGDGTTGTNAL
                     PHSSMFAGITKLVSHYTASCAIVGGGALCWGSNAEGQLGRGARGITTTLEPLPLTSVT
                     MVSQGYGHGCALGSGAVSCWGDNDYGEAGDATTLTRRLMPATVTLPITMPQHVVAAFT
                     ASCAWGAGQAACWGRNYSGQLGSGATSRALLPKRVLKPNILDGDRTVGLGMNHMCVVD
                     DQKTVTCYGNNANGQLGNGTATNSVAGASASISNVDQVVAGSMHTCARKMGTSAGEVW
                     CWGANNNGQLGDGSSTTRLSPVNVNGVGDVQKIAAGEDHTCVLNAGGQIYCWGKSNFG
                     QVGYGSTTAATTPQLVITAGATDLAVGPVSSCARVNSKWQCWGRNQDGQLATGAADGA
                     YAPTLIGAFDGADALALGDNANCKLAGGTVSCAGLLTSIGTGDNSAAIPKAPMLPGCQ
                     "
     gene            complement(4372..5943)
                     /gene="proS"
                     /locus_tag="VMZ53_28715"
     CDS             complement(4372..5943)
                     /gene="proS"
                     /locus_tag="VMZ53_28715"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HUS32531.1"
                     /translation="MSNGGKPQTAITPTRADNYPEWYLEVIKQADLAEHSGVPGCMVI
                     KPWGYSLWENIQRVLDGMFKATGHVNAYFPLFIPLSYLEKEATHVEGFAKECAVVTHH
                     RLVPSADGKGLMPDPKSKLEEPLVVRPTSETIIGASFAKWVQSYRDLPLLINQWANVV
                     RWEMRTRLFLRTAEFLWQEGHTAHSSSEEAVEETMKMLGVYADFAENYMAMPVIQGEK
                     TAGERFPGAVQTYCIEAMMQDRKALQAGTSHFLGQNFAKASGIQFTDASNQMTHAWTT
                     SWGVSTRLVGGMIMTHADDDGMICPPKLAPQHVVIMPIARKPEERQRVIDFCKALKKD
                     LEAQQYAGAPVRVHIDDRDSGGGKSWHWIKKGVPIRLEIGLRDIDADSVSMGRRDKGA
                     KDKASLKRGEIVEKVTTILDEIQTALFERARAFRKEQTRVIDSKDEFYEFFKTPAKKK
                     ESDETPPHAGFALTHFNGDPALEATIKEDLKVTVRCIPLVDRADAALGITGEPGTCPF
                     TGQPSKQRVVWAKSY"
     gene            complement(5965..8130)
                     /locus_tag="VMZ53_28720"
     CDS             complement(5965..8130)
                     /locus_tag="VMZ53_28720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32532.1"
                     /translation="MDELIGTEVGSYRLIRVLGEGGMGRVYIGEQAEIAARVAIKVLT
                     TKDPDLVARFFAEARAVNVIKHPGLVKVLALSHLSDGRPYIIMELVEGETLRTLVTRD
                     SPLPIGGVLDVMKQLLAALGAAHARGVVHRDLKPDNVMVTLDGTVKVLDFGIAKLSTT
                     LGGGNMTQVGAQIGTPAYMAPEQIRGDEVDARTDVYAAGVLLYEAITGRHPFEALSEY
                     ELLKGHLETPPPRLAARRRDAPAVLEPIVEKALAKKPAARYASTKELSAALDAAGIGT
                     QKLIARIPVTGPRTFVPNSNAPETVRERRPSAPNVDVRPTVDHKRPSQQPVAPTRVES
                     ARKMPANRVWLAGTLLVIAGGAVVVAGWPRDAPTKVVATTPAADAAAPVAVADAASVT
                     APPDARMDLARLADRSLRIDVAIDPRHFDPIANIAKAQLAARRTVSNVVLTGFRVDSA
                     HADGTLDFDDSHVVAYYFAIPNLPLDDKYSDSYCRVGVTVNASGWDVLSVLMRHCAGQ
                     EQRIPKCTLGAVWQRALAQRQKRDVPARIEWLDGRWLFAQAGTQIEIDDNCGKAAPMR
                     DVAADAGVPSAPLDASVSNAYYEYDFLIDDFEPIKMIPNATRWAHKLHPDAWLSSIHL
                     AHIDNGDVSLSNTAYQFYTSGPVAASTCTSVTVSFGSMVRVTRAEGQLCDAQKLRTPR
                     CSVAQILGRIQPRPEKPFSMTLTARGWLVGNDIYDDDCK"
     gene            complement(8155..9303)
                     /locus_tag="VMZ53_28725"
     CDS             complement(8155..9303)
                     /locus_tag="VMZ53_28725"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HUS32533.1"
                     /translation="MKALLASVALVPLMVGCTGDGLGEDGAADIQGEEKADTGPGIEV
                     TARMKPGTVDAQLSVAVPRRGYVFYAAEGAKVSLEVTQAGTAAGLDTVMKVYGPRLAD
                     GTYPKTLASDEDAGYGKLSKIRDLEITIPGFYLVEMSYSPNATPADGKKARLKLACTG
                     TCDSPLPIDTDLGLKWYRRSAERRALSAQAYSVATAQLQAKASTYTGSWGVVLDIDET
                     TLNNSTYQAERMELGLGYSPGSWTAWVERKAATPIDGVVDFTKLVHQLGGKVFFVSNR
                     MQATECAQTETNLQTAGIQYEQILCKTDTSDKNVRFAALTNGTAPSTLPPQKLVMFVG
                     DNILDFPALSQDLRKQPASAFADFGQKFILLPNSMYGSWEKNLDDNSL"
     gene            9484..10323
                     /locus_tag="VMZ53_28730"
     CDS             9484..10323
                     /locus_tag="VMZ53_28730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32534.1"
                     /translation="MKIVEAKMKNVFVVCVALSAALASVGCKKKADSGDDAFAKMSSF
                     KDSMCACKDKACADKVSDDMTKWSEEMSKKAGDKKPAAMSEADTKKYAEVTTKLGECT
                     TKAMTAGADTMAGSAAPSGSTTPAVATVKVGEGPVVPAKTLALYDYPAFKGVPAEALW
                     TNVEAEKDGDKFQNLMVGKDGYVSMRIIDCNSPKLKEYAAKAEDSGDFKWCFKTPAAD
                     AQKAGEYPRLDAADDAVVVIKADHLVVQMGIWGGKEDKIKKDDLAAYLATVDFKSMLA
                     TAK"
     gene            complement(10416..11081)
                     /gene="tsf"
                     /locus_tag="VMZ53_28735"
     CDS             complement(10416..11081)
                     /gene="tsf"
                     /locus_tag="VMZ53_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HUS32535.1"
                     /translation="MEVTATAIKDLRERTGAGMADCKKALTETSGDMDKAIDYLRAKG
                     LAKAAKKAGREATEGAVVSYIHAGGRIGVLVEVNCETDFVARNEDFMGFTRDVAMQIA
                     AMAPQFVRKEEIPAEYVEKEKAVLIAKAKEDPKNANKPDQILSKITEGQVTKWMKEIC
                     LLDQPFVKDNDKTIEQLQQELVAKIGENIKIRRFVRFELGEGLEKKKSDFAAEVAQQA
                     GLA"
     gene            complement(11084..11908)
                     /gene="rpsB"
                     /locus_tag="VMZ53_28740"
     CDS             complement(11084..11908)
                     /gene="rpsB"
                     /locus_tag="VMZ53_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HUS32536.1"
                     /translation="MRALLEAGVHFGHNTGRWNPKMKQFIFGARNGIHIIDLQHTVKL
                     FRQAFQAVVDTTARGDTIMFVGTKKQAQDVIVEEASRSGQHYVTQRWLGGTLTNFKTV
                     KGSLERLRSLEKMEEDGTLFALTKREQVMIRRDRDKLLKSLGGIKGMSKLPGMLFVID
                     PHKEHIAVDEARKLEIPVVAITDTNCDPDQIDYVIPGNDDAIRAIKLFASKIADAAII
                     GSKVWKETARHRGAERQDEGERVIHVSSGGDGPRVEMSTGGSMFNPEASASPDAES"
     gene            12160..>12979
                     /locus_tag="VMZ53_28745"
     CDS             12160..>12979
                     /locus_tag="VMZ53_28745"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32537.1"
                     /translation="MRSSLALILFVAACGSSKPATQPPPPPADNTVADNTQQQTPPAS
                     DDLPPVDKPTKPVKNASLKEIGLDPDALDRTVDPCEDFYQFACGGWIKKTEIDADKPL
                     AMRSFIDIEERNYDYLHGLLDKPKSTPETKPLMDKLTAFYGSCMDTATIDKAGLKPVK
                     PLLDKINKIKDTKTLSTAIIELQTAGANALFGFGPTEDYANAQNVIAGLDQAGLGLPD
                     RDYYLKDEHKAMRDAYVELVTALLVEAGHNPVSAKTEAADVLALETEMAKVSMDK"
BASE COUNT         2234 a         4193 c         4208 g         2344 t
ORIGIN      
        1 tgcggtcgtc gtgctcgacg gcgttgctgg cgtagagcca cagacggaga ccgtgtggcg
       61 gcaagcggac aggtaccgcg tgcctcgcat cgtgttcgtc aacaagctcg accgcgttgg
      121 cgccgactat cagcgatcac tcgacaagct gcgctcgcgc ttcggcgtgc gtgccgtcgc
      181 cgtcacgtgg ccgctgtatg agaacgatgt gctcgtcggc gtgatcgacc tcgtcgacca
      241 aacggaggtt cgttggtcgg acgacgcggg gttcgcgttc agcgcgacac ccgtgacgct
      301 ctctgacaac ttgcaagcta cgcgtgaccg tctgctcgag gcggctgccg acgaggatcc
      361 cgagattctc gccgcggtcg tcagtgggct ggcgattccg cctgctcgat tgcgggctgc
      421 gttgcgacgc ggcaccgtga gcggccggct cgtgcccgtg ctcgctggtt cggcgtacaa
      481 gaaccgcggc gttcagccat tgctggatgc ggtggtctcg tatctgccgt cgcctagcga
      541 tcgcgagccc gtgaaggctg ctgacggatc ggccgagcgc aagccatcgc gaaccgagtc
      601 actggccgcg cttgcgttca aggtcgtctt cgacgaccat ggacagctgt cgttcgtgcg
      661 cgtgtactcg ggcgtgctcg cgcgaggaga gacgatcgtg accggtcgcg gccgcaagct
      721 gcgaatcggc cggctcgtgc agctcgtcgc ggcggagcaa gtcgaagtcg acgcgctcgg
      781 cgcgggtgaa atcggcgccg tcctcggtgc gccgcttccc ggtggcgaaa cgctctccga
      841 tccagcgcag ccgatcgtcc tcgaggcgat caccgcgccg gatccggtga tgcgcgtcgc
      901 gatcgaagcg cgcaccgcag cggaacgcga caagctcgcg ctcgcactcg gacgcatggt
      961 cgcggcggat ccatcgctgc gactcgagag cgatcaagag acgggacaga ccctgcttgc
     1021 aggcatgggt cagctccatc tcgacgtcgc gatcgaacgt cttgcgaccg agcatcgcgt
     1081 tcacgtcacg gccggtgcgc cgcgcgtcgc gtatcgcacg acgttgacga agaccgtgca
     1141 gcgagagctg cgccatatcc gtcagcgcgg cggtccgggc caatacgctc acgtcgtcgt
     1201 cgaagtgggt cctgcgccga gaggcagcgg gctcctcttc gaggacaaga cgaagggcgg
     1261 cgtcgtcccg aagtcgtacg cgaaagcagt cgaggctggc gtgcgcggcg cgatgagtga
     1321 aggtcttctc ggcggtcacc ctgtggtcga cgtgaaggtc tcgctcctcg acggttccac
     1381 gcacgagaag gactcgaacg agcaggcgtt tacggtcgcg ggctcactcg cgttcaaagc
     1441 ggcggcggcc gatacggcgc cgatcaagct cgagccgatc atgctcctcg aggtcagcgt
     1501 gcccgaggac gaggtcggca acgtgatcgg cgatctcggt cgacgacgtg gcgaagtgct
     1561 cgcactcgat gcgcagggtg cagatcgtgt ggtgcgtgcc gaggtcccgc ttgcggagac
     1621 gttcggttac gccagcgcgc tcggcaatct cacgcacggt cgtggccgct tcacgctcga
     1681 acccgctcgc tacgagccag tgcgttagtt ggaagggcgc agggaggaag ctctctgcgc
     1741 ccgttgttta ttttcggtcg aacagccggc ggagcttgtt catcagcgtt tcgtgcggcg
     1801 gcaggtcggc gcggaactct ttcgcttcgg acagcagctc ggcgagctct ttgctgtcga
     1861 accacacgcc gtgcggctcg tgacggtcga ggttcaccgt gccgagcttc accgtctgca
     1921 tgggcgcgcc gcactcggga cacacgcggt ggtgatcctc gctgctcgat tgccaatcca
     1981 acgatacgag cgtcgccgcg ctctgctcga ggagcggcac catcacctcg gcgcgaaccc
     2041 acgaaccgtc acacgtctcg cacttgtagg tgcggccggc ctgctcgagc gcggacttgc
     2101 agatggggca ctgcatcgca gcatcgtggc acggcgagcc ggtacaatcg agtggatgac
     2161 gtggcgtcgc gcgttggtgt tcgcctgcac gctcgcggga tgcgatcgcc tcttcgcgat
     2221 cacgcacgtt cccgatccgc cgctcgacgc ataccagcct gatgcggcgc cgccgtgcct
     2281 cgtgaacgac atcgctgtcg ggcgtgcgca cgcgtgcgcg atcattccca gtggcgacgt
     2341 ctactgctgg ggcgagaacg ggtacggcca gatccagccc agcggcccgg agtacgtgct
     2401 gacgcccgca aagatctttc tgcccgggcc agcggcagaa atcgccctgg gacgaacgca
     2461 cacgtgcgcg cgcgttggcg gcgatgtgta ttgccagggc cagaacaact ccggtcagct
     2521 cggcaagggt ccaggcgaca ccagccccgc gcttcgcaag gtcgatggta ttccgccgag
     2581 cgcgcaaatc gcggccggcg gtgcgcacgt gtgctcgcgc agcgcgacgg atggcgcggt
     2641 ctggtgctgg gggcgcaacc gtcacagcga gacaggaggc acgagcgtgc tctgtagcgg
     2701 caacactgcg tgcgcgctgc ccggcgcggt tcccgggatc acgggcgcga agaccgtcgt
     2761 cgccgggcac accggtacct gcatcatcga cgcaagcgat cagctcttct gttggggacg
     2821 caacgactac ggccagctcg gcatcggcaa ctcgatcgga acggcgacgc caaccctcgc
     2881 gatggtgact cctcccgtac gcgccgtcga catgggcggc aagaacatgt gcgccatcga
     2941 cggcatgcat cgcgcgttct gcagcggcga aggcgagctc ggcacgcttg gcaacggcgg
     3001 ctttcaagcg acctcgacac cggtgcaggt cttcaccgga gtccccgtgc agatttcttc
     3061 agcaagcttt ggtgcgtgcg ccgtcaccga cgacgggatg ctcagctgct ggggacgagg
     3121 cgagactgga gacggcacca cgggcacgaa cgcgcttccg cattcgtcga tgttcgcggg
     3181 cattacgaag ctcgtgagcc actacacggc gagctgcgcg atcgtcggcg gaggagcgct
     3241 ctgttggggt agcaatgcgg aaggtcagct cggccgcggt gcgcgcggca tcacgacgac
     3301 gctagaaccg ctaccgctga cgtcggtgac gatggtgtct caggggtacg ggcacggctg
     3361 cgcgctcggc agcggtgccg tgagttgctg gggtgacaac gactacggcg aagccggcga
     3421 tgcaacaacg ctcacgcgcc ggctaatgcc cgcgactgtc acgctgccga tcacgatgcc
     3481 gcaacacgtc gtcgctgcgt tcactgcttc atgcgcatgg ggagcgggcc aagcagcatg
     3541 ctggggacgt aactacagcg gtcagctcgg cagtggggcg acgtcacgcg cgctcctccc
     3601 aaagagagtc ctgaaaccga acatcctcga cggcgatcgc accgtcgggc tcggcatgaa
     3661 tcacatgtgt gtcgtcgacg atcagaaaac cgtcacgtgc tacggcaaca acgcgaatgg
     3721 ccagctcggc aacggcacgg ccacgaactc cgtcgccggc gcgagtgcct cgatctcgaa
     3781 cgtcgatcag gtcgtcgccg gcagcatgca cacgtgcgcg cggaagatgg gaacgagcgc
     3841 aggcgaggtc tggtgctggg gcgccaataa caacggacaa ctcggcgacg gcagctcgac
     3901 cacccgactc tcgccggtca atgtgaatgg cgtcggcgac gttcagaaga ttgccgcggg
     3961 cgaggaccac acgtgtgtgc tcaacgccgg aggccagatc tactgctggg ggaagagcaa
     4021 cttcggtcag gtcggctacg gctcgacgac cgccgccacg acaccgcaac tggtcatcac
     4081 ggctggagca acggatctcg ccgtcgggcc tgtttcgagc tgcgcgcgag tgaattcgaa
     4141 atggcaatgc tggggtcgta accaggacgg ccaacttgcg acgggcgccg cggacggcgc
     4201 gtacgccccg acgctcatcg gcgcgttcga cggcgcagac gcgctcgcgc tcggcgataa
     4261 cgcgaactgc aagctcgcgg gcggcacggt ctcgtgcgca ggccttctca catcgatcgg
     4321 caccggcgac aattcggccg cgatcccaaa ggcgccgatg ctcccgggct gtcagtaaga
     4381 cttcgcccag acgacgcgct gcttgctcgg ctgtcccgtg aacgggcacg tgccgggctc
     4441 gccggtgatt ccgagcgcgg cgtccgcacg gtcgacgagc gggatgcaac gcacggtcac
     4501 cttgaggtct tccttgatcg tcgcctcgag cgccggatcg ccattgaagt gcgtgagcgc
     4561 gaagcccgcg tgcggcggcg tctcgtcgct ctccttcttc ttcgcgggcg tcttgaagaa
     4621 ctcgtagaac tcgtccttcg agtcgatgac gcgcgtctgc tccttgcgga acgctcgcgc
     4681 acgttcgaat agcgcggtct ggatctcgtc gaggatcgtc gtgaccttct cgacgatctc
     4741 gccacgcttg agcgacgcct tgtccttcgc gcccttgtcg cggcgtccca tcgagacgct
     4801 gtcggcgtcg atgtcgcgca gcccgatctc gaggcgaatc ggcacgcctt tcttgatcca
     4861 gtgccagctc ttcccgccgc cggaatcacg atcgtcgatg tgcacgcgca ccggtgcgcc
     4921 ggcgtactgc tgcgcttcga gatccttctt cagcgccttg cagaagtcga tgacgcgctg
     4981 gcgctcctcg ggcttgcgcg cgatcggcat gatcacgacg tgctgcggag cgagcttggg
     5041 cgggcagatc atgccgtcgt cgtcggcgtg cgtcatgatc atgccgccga cgaggcgcgt
     5101 cgacacgccc cacgacgtcg tccatgcgtg cgtcatctgg ttgctggcgt cggtgaattg
     5161 gatccctgac gccttcgcga agttctgccc caggaagtgc gacgtgccgg cctgcagcgc
     5221 cttgcgatct tgcatcatcg cttcgatgca gtacgtctgc acagcgccgg ggaaccgctc
     5281 gcccgccgtc ttctcgcctt ggatcacggg catcgccatg tagttctcgg cgaagtcggc
     5341 gtacacgccg agcatcttca tcgtctcctc gacggcttcc tctgaagacg agtgagcggt
     5401 atggccttct tgccagagga actcggcggt gcgcaggaac agacgcgtgc gcatttccca
     5461 gcggacgacg ttcgcccatt gattgatgag cagcggcagg tcgcgatagc tctgcaccca
     5521 cttcgcgaac gacgcaccga tgatcgtctc cgacgtcgga cgcacgacga gcggctcctc
     5581 gagcttgctc ttcggatcgg gcatcagacc cttgccatcg gcactcggca cgagccggtg
     5641 atgcgtgacg accgcgcact ccttcgcgaa cccttcgacg tgcgtcgctt ccttctcgag
     5701 gtacgagagc gggatgaata gcgggaagta cgcgttgacg tggcccgtcg ccttgaacat
     5761 gccgtcgagg acgcgctgga tgttctccca cagcgagtag ccccacggtt tgatcaccat
     5821 gcagccagga actcccgagt gctcggcaag atctgcttgc ttgatgacct cgaggtacca
     5881 ctcagggtag ttgtcggcgc gggtcggtgt gatcgcggtt tggggcttgc cgccgttgct
     5941 catagccgcg gaggcatacc atgcctactt gcagtcgtcg tcgtagatgt cgttgccaac
     6001 gagccagccg cgagcagtaa gcgtcatgct aaacggcttc tcgggacgcg gctgtatgcg
     6061 ccccagaatc tgcgccaccg agcagcgcgg cgtccggagc ttttgcgcat cgcacagctg
     6121 cccctcggcg cgtgtaaccc gcaccatcga gccaaagctc accgtcaccg atgtacatgt
     6181 cgacgccgcg acaggaccgc tcgtgtagaa ctggtacgcc gtattggaga gcgaaacatc
     6241 gccgttgtcg atgtgcgcga ggtggatcga gctgagccac gcgtcggggt ggagcttgtg
     6301 cgcccaccga gtcgcattgg ggatcatctt gatcggctcg aagtcgtcga tcaggaagtc
     6361 gtattcgtag tacgcgttcg acacgctcgc atcgagcggt gcgctcggaa cgcctgcatc
     6421 ggctgcgacg tctcgcatcg gcgccgcttt accgcagttg tcgtcgatct cgatctgggt
     6481 acccgcttgg gcaaacagcc agcgaccatc gagccactcg atgcgcgccg gcacgtcccg
     6541 cttctgacgt tgtgcaagcg cacgctgcca gacagcgccg agcgtgcact tcggaatccg
     6601 ctgctcttgg cctgcgcaat ggcgcatcag gacgctcaac acgtcccagc ccgaggcgtt
     6661 cacggtcacg ccgacacgac agtacgaatc gctgtacttg tcatcgagcg gaagattcgg
     6721 gatcgcgaag taataggcga cgacatggga gtcatcgaaa tcgagcgtac cgtcggcatg
     6781 cgcgctgtcg acgcgaaacc ccgtcaagac cacgttcgag accgtgcgtc gcgccgccag
     6841 ctgtgccttc gcgatgttcg cgatcggatc gaagtgccgc ggatcgattg caacatcgat
     6901 gcgcagactg cgatccgcga ggcgcgcaag gtccatccgc gcgtcgggcg gcgccgtcac
     6961 gctggcggca tcggcgacgg caacgggtgc agcggcatcg gccgccggcg tggttgcgac
     7021 gaccttggtc ggcgcgtcac gaggccatcc ggcaacgacg accgcaccac cggcgatcac
     7081 gagcaacgtc cccgcaagcc acacgcgatt cgcgggcatc ttgcgcgcgc tctccacgcg
     7141 cgtcggggcg accggctgtt gcgacggcct cttgtgatcc accgtgggcc gcacgtccac
     7201 gttcggcgcg ctcggccggc gctctctcac ggtctcgggc gcgttgctat tcggcacgaa
     7261 cgtccgcggc cccgtcaccg gaattcgcgc gatgagcttt tgcgtgccga tacccgcggc
     7321 gtcgagggcc gccgacagct ccttcgtgct tgcgtagcga gcggcaggtt tcttcgcgag
     7381 cgccttctcg acgatcggct cgagcacagc gggcgcatct cgacggcgtg cagcgagccg
     7441 aggcggcggc gtctcgaggt gtcccttcaa cagctcgtac tcgctcagcg cctcgaatgg
     7501 atgacgcccg gtgatcgcct cgtagagcaa tacgcccgcc gcgtacacgt cggtgcgcgc
     7561 atcgacctcg tcgccgcgga tctgctcggg tgccatatac gcaggcgttc cgatctgcgc
     7621 gccgacttgc gtcatgttgc cgccgccgag cgtcgtcgag agctttgcga tcccgaagtc
     7681 gaggactttg acggtgccgt cgagcgtcac catcacgttg tcgggcttga gatcgcgatg
     7741 cacgacgcct cgcgcgtgag cggcaccgag tgcagcaagc agctgcttca tcacgtcgag
     7801 cacgccgccg atcggcaacg gcgaatcacg cgtgacgagc gtccgcagcg tctcgccctc
     7861 gacgagctcc atgatgatgt acggccggcc gtcggaaaga tgcgacagcg cgagcacctt
     7921 cacgaggccc gggtgcttga tgacgttgac cgcgcgcgcc tccgcgaaga accgcgcgac
     7981 gagatccgga tccttcgtcg tgaggacttt gatcgcgacg cgtgccgcga tctccgcctg
     8041 ctcgccgatg tagacgcggc ccatgccgcc ctcgccgagc acgcgaatca gccggtagct
     8101 accgacctcg gtcccgatca gctcgtccac gcgggtcgag catatcgcgc gacgctacag
     8161 cgagttgtcg tcgaggttct tttcccagga gccgtacatc gagttcggca gcaggatgaa
     8221 cttctgcccg aagtcggcga acgcgctcgc cggctgcttg cggaggtctt gcgacagcgc
     8281 cgggaagtcg aggatgttgt cgccgacgaa catcacgagc ttctgcggcg gaagcgtcga
     8341 cggcgccgtg ccattcgtca gcgcggcgaa gcgcacgttc ttatcgctcg tgtcggtctt
     8401 gcagagaatc tgttcgtact ggatgcccgc cgtctggagg ttcgtttcgg tctgcgcgca
     8461 ctccgtcgct tgcatgcgat tcgagacgaa gaacaccttg ccgccgagct ggtgcacgag
     8521 cttggtgaag tcgacgacgc catcgatggg agtcgcggcc ttgcgctcca cccatgcggt
     8581 ccagctgccc ggcgagtaac cgaggccgag ctccatgcgc tccgcttgat acgtcgagtt
     8641 gttgagcgtg gtctcgtcga tgtcgagcac gacgccccac gagccggtgt acgtcgacgc
     8701 cttcgcctgc agctgcgcgg tcgcgaccga gtacgcctgc gcgctcagcg cacgacgctc
     8761 ggccgagcgg cgataccact tgagcccgag atccgtatcg atcggcagcg ggctgtcaca
     8821 cgtgccggtg cacgcgagct tgaggcgcgc cttcttgccg tcggccggag tcgcgtttgg
     8881 cgagtagctc atctcgacga ggtagaaacc gggaatcgtg atctcgagat cgcggatctt
     8941 cgagagcttg ccgtagccgg cgtcttcgtc ggacgcgagc gtcttgggat acgtgccgtc
     9001 cgcgagccgc ggaccgtaga ctttcatcac cgtgtcgagc ccggcggcgg tacccgcctg
     9061 ggtgacctcg agcgacacct tcgcgccttc ggccgcgtag aacacgtagc cgcgacgagg
     9121 caccgcgacg gacagctgcg cgtcgaccgt gccgggcttc attcgcgcgg tcacttcgat
     9181 acccggaccc gtgtccgcct tctcttcgcc ttggatatcc gctgcgccgt cctcaccgag
     9241 cccatcgccc gtgcaaccga ccatcaacgg aaccaacgcc accgacgcca acaatgcttt
     9301 catgatgcgg gcgcgcttct gcagtcgcta tgcccagttg cgtccaagca tgttcgtgcg
     9361 cttacggttc gccgacgcgg caactcgagt agccaggcaa ctgacacttg tcgcgctctg
     9421 cggacgatcc ggcgtcaatg cgccgacccc cgtcgtgcgc gtgacgctgt ataagagccg
     9481 ctcatgaaaa tcgttgaggc aaaaatgaag aacgtgtttg tggtgtgtgt ggctctcagt
     9541 gcggcgcttg cgtccgtggg ctgcaagaag aaggcggaca gcggcgacga cgcgttcgcc
     9601 aagatgtcct cgttcaagga ctcgatgtgc gcgtgcaagg acaaggcgtg cgctgacaag
     9661 gtctcggacg acatgacgaa gtggtcggag gagatgtcga agaaggctgg cgacaagaag
     9721 ccggccgcga tgtccgaagc ggacacgaag aagtacgcgg aggtcaccac caagctcggt
     9781 gagtgcacga cgaaggccat gaccgcagga gcggatacga tggcaggctc ggcggcgccc
     9841 tcgggctcca cgactcccgc ggtcgcgact gtgaaggtcg gcgagggccc cgtggttccc
     9901 gcgaagactc tcgcgctcta tgactatccc gcgttcaagg gcgtgcctgc cgaggcgctg
     9961 tggaccaacg tcgaggccga gaaggacggc gacaagttcc agaacctgat ggtcggcaaa
    10021 gatggttacg tcagcatgcg catcatcgac tgcaactcgc cgaagctgaa ggagtacgcc
    10081 gcgaaggcgg aggacagcgg cgactttaag tggtgcttca agacgccggc tgcggacgcg
    10141 cagaaggccg gcgagtaccc gcgcctcgac gccgcggatg acgccgtcgt cgtgatcaag
    10201 gccgaccatt tggtcgtcca gatgggcatc tggggcggca aggaagacaa gatcaagaag
    10261 gacgatctgg ccgcctacct cgcgacggtc gacttcaagt cgatgctcgc aaccgcgaag
    10321 tagttagccc gctttcgcga gaaagcgccg aaaacaaaag cgccggcacg aagccggcgt
    10381 tttttttgcg ctcgctttcg cgagggcagc gagactcagg ccaggccggc ctgctgtgcg
    10441 acctcggctg cgaagtcgct cttcttcttc tcgaggcctt cgccgagctc gaagcggacg
    10501 aagcggcgga tcttgatgtt ctcgccgatc ttcgcgacga gctcctgctg gagctgctcg
    10561 atcgtcttgt cgttgtcctt cacgaacggt tggtcgagga gacagatctc cttcatccac
    10621 ttcgtgacct gaccctcggt gatcttcgag aggatttgat ccggcttgtt cgcgttcttc
    10681 ggatcttcct tcgccttcgc gatcaggacg gccttctcct tctcgacgta ctcggccgga
    10741 atttcctctt tgcgaacgaa ctgcggcgcc atcgcggcga tctgcatcgc gacgtcacgc
    10801 gtgaagccca tgaagtcttc gttgcgcgcg acgaagtccg tctcgcagtt cacttcgacg
    10861 agcacgccga tacggccgcc cgcgtggatg tacgaaacga cagcgccctc ggtggcctca
    10921 cggccggcct tcttcgccgc cttcgcgaga cccttcgcgc gcaggtagtc gatcgccttg
    10981 tccatgtcgc cggacgtctc ggtcaaagcc ttcttgcagt cggccatgcc cgcgccggtg
    11041 cgctcgcgca gatccttgat tgcagttgca gtgacttcca tctctacgac tccgcgtcgg
    11101 gcgaggccga ggcctcgggg ttgaacatgc tgccgcccgt cgacatctcg acgcgcggac
    11161 catcgccacc cgacgaaacg tgaatgacgc gctcgccttc atcctggcgc tctgcaccac
    11221 gatgacgagc agtctctttc cagaccttgc tgccgatgat cgcggcgtcg gcgatcttcg
    11281 acgcgaacag cttgatcgcg cggatcgcgt cgtcgttgcc ggggatcacg tagtcgatct
    11341 gatccggatc gcagttcgtg tccgtgatcg cgacgacggg aatctcgagc ttgcgagcct
    11401 cgtcgacggc gatgtgctct ttgtgcgggt cgatgacgaa gagcatgccc gggagcttgc
    11461 tcatgccctt gatgccgccg agcgacttga ggagcttgtc gcggtcgcga cggatcatca
    11521 cctgctcacg cttcgtgagc gcgaacagcg tgccgtcctc ttccatcttc tcgagcgagc
    11581 gaagacgctc gagcgagccc ttcaccgtct tgaagttggt gagcgtgccg ccgagccaac
    11641 gctgcgtcac gtagtgctga ccagagcggc tcgcctcttc gacgatgacg tcctgcgcct
    11701 gcttcttggt gccgacgaac atgatcgtgt caccgcgcgc ggtcgtgtcg accacggcct
    11761 ggaaggcctg gcggaacagc ttgaccgtgt gctgcaggtc gatgatgtgg atgccgttgc
    11821 gcgcgccgaa gatgaactgc ttcatcttgg ggttccaacg acccgtgttg tgtccgaagt
    11881 gcacgcccgc ttcgagaagg gcgcgcatgc tgatggggtt ttgccccgtc gcttctgcca
    11941 tcgtttccat gtcgatcacc ttttggtttt gagccacccc tctcttcact gaagccggaa
    12001 accccgaagg gccaccaccg gttatctctg agagggtgag tagttctgcg atgattcgca
    12061 gaggcgggtg atctaccccg acgccgcggc ctttacaagg cctctcggct atcacatgaa
    12121 cctaataaaa cggcggactt atgtgctatc gcaacgccca tgcgcagctc cctcgcgttg
    12181 atcctgttcg tcgccgcctg cggttccagc aaacctgcga cgcagccgcc gccgccgccg
    12241 gccgacaaca cggtcgcgga caatacgcag cagcagacgc cgccggcgtc cgatgatctg
    12301 ccgcccgtcg ataagccgac gaagcccgtg aagaacgcgt cgctcaaaga gatcgggctc
    12361 gatcccgacg cgctcgaccg caccgttgat ccttgcgagg acttctacca attcgcgtgc
    12421 ggcggctgga tcaagaagac cgagatcgac gccgacaaac cgctcgcgat gcgcagcttc
    12481 atcgacatcg aagagcgcaa ctacgactac ctccatggtc tgctcgacaa gccgaagtcg
    12541 acgcccgaga cgaagccgct catggacaag ctgacggcgt tctacggctc gtgcatggac
    12601 acggcaacga tcgacaaggc cggactcaag ccggtgaagc cgctgctcga caagatcaac
    12661 aagatcaaag atacgaagac gctctcgacc gcgatcatcg agctgcagac cgccggagcg
    12721 aacgcgctgt tcggattcgg cccgacggaa gactacgcga acgcgcagaa cgtgatcgca
    12781 ggtctcgatc aagcgggcct cggtctgccc gatcgcgact actacttgaa ggacgagcac
    12841 aaggcgatgc gcgatgcgta cgtcgagctc gtcaccgcgc ttctcgtcga ggcgggccac
    12901 aatccagtct cggcgaagac cgaagcggcc gacgtgctcg cgctcgagac ggagatggcg
    12961 aaggtctcga tggacaagg
//
LOCUS       DATLBZ010000581         5101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10852368, whole genome shotgun sequence.
ACCESSION   DATLBZ010000581 DATLBZ010000000
VERSION     DATLBZ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10852368"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1050
                     /locus_tag="VMZ53_28750"
     CDS             184..1050
                     /locus_tag="VMZ53_28750"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF006492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HUS32538.1"
                     /translation="MSVIGIVGGTGLYALEGLTEQRWKRVDSPFGTPSDELLFGRVGE
                     QEIVFLPRHGRGHRIPPHEINFRANIDALKRAGATSLISVSAVGSLKPELTPGTFVIV
                     DQFIDRTLRPNKSFFTTGLVAHVSMGHPTCRRLMDHLAVAATSVGVTAVKGGTYVVME
                     GPQFSSMAESELHRSWRADVIGMTNMPEAKLAREAELCYATVAMVTDFDCWHPDHDHV
                     QVADIVKVLNANSANARRLVADVIPRLAADSGPCTQGCDRALEFAIMTAPEARDAALV
                     AKLDAVAGRVLK"
     gene            1047..2063
                     /gene="mtnA"
                     /locus_tag="VMZ53_28755"
     CDS             1047..2063
                     /gene="mtnA"
                     /locus_tag="VMZ53_28755"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HUS32539.1"
                     /translation="MKVDGRWRRTIELDASGTVIVLDQSRLPYDIEWLPLRTLDDAAS
                     AIRDMKIRGAPLIGATAAYGVAIALRDGATLEHACEVLARTRPTAINLRWALDEMRRR
                     VVDYDPFAAAALIADEDVATCEAIGTHGLELIKRIDKPRVNVLTHCNAGWLATVDWGT
                     ALAPVYKAHEAGIDVHVWVDETRPRNQGLLTAWELKQHGVPHTVIADNAGGHLMQQRQ
                     VDLCIVGTDRTTKNGDVCNKIGTYLKALAAHDNAIPFYAAVPSSSIDFTVGEPHEIPI
                     EERSGDEVRLIRGASVIAADTAVANPAFDVTPRRLMTGLITERGIADPAAASLAALFP
                     ERTR"
     gene            2060..2710
                     /locus_tag="VMZ53_28760"
     CDS             2060..2710
                     /locus_tag="VMZ53_28760"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUS32540.1"
                     /translation="MTMRLRDDLVSTAKKMSALGLTPGMSGNVSVRTSQGFLITPSGM
                     PYGDLIPDDVVDIKHDGAIRPGQKTPSTEWQLHRDILGARQDVEAIVHTHSLYCTSIS
                     TLRQPIPAIHYMVVLAGSDEIPCADYATFGSTQLALNAVTALRGGNACLLANHGMVAL
                     GKTLPEALRLAAEVETLAGQYWHAAQIGTPYILDGDELQRVRAKFAEYGQKKPRRY"
     gene            complement(2704..3489)
                     /locus_tag="VMZ53_28765"
     CDS             complement(2704..3489)
                     /locus_tag="VMZ53_28765"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUS32541.1"
                     /translation="MNRERLQRIRNFQSEDWYPALIIRPLTILIMLVIADWRPLTPNR
                     VTTLANLSKLAAAWLILDKQHWLAAALLLQVGLIFDHLDGTIARYRRVFTKFGSFYDK
                     ASDMVTWTVIMMAVGWKETELTGDNKWTLFAAISVVTLNLRGYTKWLEVAEGERLRWL
                     EAKADPVAAVAKRTAPIVIKPPPVRTAKDWALWFLKKVAVVFVFEEADLWFWLGLALL
                     IDRLDLVLWLFMISQSAGAAIMFVVRSISMTRIDRKIRELESQ"
     gene            3568..5034
                     /locus_tag="VMZ53_28770"
     CDS             3568..5034
                     /locus_tag="VMZ53_28770"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HUS32542.1"
                     /translation="MSAQQRMNTNVNRGLAWLGVASSLVGLLDIVSILIILNNWISAE
                     EYGIATKCIWIFPVLDQATDLGLSAAVVQRDDHTPRRISTVFWINVMLSLALFALIAI
                     TAPILAVNFYGHAIVGWMLIAYGGKLILQNGYFIPWAMMKRELRFKELSALRISANVV
                     EFIAKIGFAATGFGIWCFVLAPLLRTVTYLIGTQLRHRYRPRWEFSYPEAKEYVTFGL
                     RSSGSQILFFFYSNVDYPIVGYFFGDVALGFYRLAYEIVLEPVRVISNVVVDVAFPAF
                     AKLRANTAALVDQFVSFTRLNLITVMSYSAIVFVAAPDVLALLFPSYKGSTEAVQILC
                     AVAVLRAVGFVAPPLLDGVGHPERTLRYMTAAAVAMPLAFTLGAILLGPRLGFESVAA
                     AWALGYPIAFAVLIYMTVVTLNWSVAKYVRAVGGVALCMLAAGGVAAVVYPLLPAGHG
                     VRALVTTVVVVVVSALLLAYTQGLTLRGVVRSMREPPP"
BASE COUNT          869 a         1724 c         1593 g          915 t
ORIGIN      
        1 ttcggcatga ccgtgcttgc cgtgcgcaag cccaccgacg acgcttggaa ctacaacccc
       61 gacgctaccg tcacgatcgg tccgggcacg acgctcgtcg tcctgggctc atccgagcaa
      121 gtcgcagagc tacgccgcca gggtggctag tcgacgatat cgagcaggtg gtatctccgg
      181 atcatgtccg ttatcggcat cgtcggaggc acaggcctat acgcgctcga gggattgacc
      241 gagcagcgct ggaagcgcgt cgattcgccg ttcggcacgc cgtcggatga gctgctgttt
      301 gggcgggtgg gcgagcagga aatcgtcttc ttgccgcgcc acggccgcgg tcatcgtatc
      361 ccgccgcacg agatcaactt ccgcgcgaac atcgacgcgc tgaagcgggc aggggccacg
      421 tccctcattt cggtcagcgc cgtcggttcg ctcaagccgg agctaacgcc tggcacgttc
      481 gtgatcgtcg accagttcat cgatcgtacg ctgcggccga acaagtcgtt cttcacgacg
      541 gggctcgtcg cgcacgtctc gatgggccac ccgacgtgcc gccgtctgat ggatcatctc
      601 gcggtggcag cgacctccgt cggcgtgacc gcggtcaaag gcggcaccta cgtcgtgatg
      661 gagggcccgc agttctcgag catggcggag tccgagctgc accgtagctg gcgcgccgac
      721 gtcatcggca tgacgaacat gcccgaggcc aagctcgcgc gcgaagccga gctttgttac
      781 gcgaccgtcg cgatggtgac cgacttcgat tgctggcatc ccgaccacga tcacgtgcag
      841 gtcgccgaca tcgtcaaagt cctcaacgcg aacagcgcca acgctcgccg cctcgtcgcg
      901 gatgtcatcc cacggcttgc cgccgacagt gggccgtgca cgcagggctg cgaccgtgcg
      961 ctcgagttcg cgatcatgac ggcacccgag gcacgcgacg ctgcgctcgt cgccaagctc
     1021 gacgctgtag cggggcgcgt cctgaaatga aagtcgatgg ccgctggcga cgcacgatcg
     1081 agctcgacgc gagcggcacg gtcatcgtgc tcgatcagtc gcggctgccg tacgacatcg
     1141 agtggctgcc gcttcgcacg ctcgacgacg cggcgtccgc catccgcgac atgaagatcc
     1201 gcggcgcgcc gctcatcggt gcaacggccg cgtacggcgt cgcgatcgcg ctgcgagacg
     1261 gcgctacgct cgagcacgcg tgcgaggtgc tcgcgaggac gcgtcccacc gcgatcaacc
     1321 tgcgctgggc actcgacgaa atgcgtcgtc gcgtcgtcga ctacgatcca ttcgccgccg
     1381 cggcgttgat cgccgacgag gatgtcgcga cctgcgaggc catcggcacc cacggtctcg
     1441 agctcatcaa acgtatcgac aagccgcgcg tcaacgtgct cacgcattgc aacgcgggct
     1501 ggctcgccac cgtcgactgg ggcaccgcgc tcgcgccggt ctacaaagcg cacgaagccg
     1561 gcatcgacgt ccacgtgtgg gtcgacgaga cacgcccgcg caaccaagga ctcctcacgg
     1621 cgtgggagct caaacagcac ggcgtgccgc acaccgtgat cgccgacaac gcaggcggcc
     1681 atctgatgca acagcgccaa gtcgacctct gcatcgtcgg caccgatcgc acgacgaaga
     1741 acggcgacgt ctgcaacaag atcggcacgt acctcaaggc gctcgccgcg cacgacaacg
     1801 cgatcccgtt ttacgccgcc gtcccatcat cgagcatcga cttcaccgtc ggtgagccgc
     1861 acgaaatccc gatcgaagaa cgctcgggcg acgaagtgcg cctcatccgc ggcgcatccg
     1921 tcatcgccgc cgacaccgcg gtcgctaatc ccgcgttcga tgtcacgccg cgccgcctca
     1981 tgaccggcct catcaccgag cgcggcatcg ccgacccagc tgccgcgtcg ctcgccgcgc
     2041 tgttcccgga gcgcacgcga tgacgatgcg cctgcgcgac gacctcgtct cgacggccaa
     2101 gaagatgtcg gcgctcggcc tcacgccggg catgtccggc aacgtcagcg tgcgcacgtc
     2161 gcagggcttc ctcatcacgc catcgggcat gccgtacggt gacctcattc ccgacgatgt
     2221 cgtcgacatc aagcacgacg gcgcgatccg ccccggccag aagacgccgt ccaccgagtg
     2281 gcagctccac cgcgacatcc tcggcgcgcg ccaagacgtc gaagcgatcg tccacacgca
     2341 ctcgctctac tgcacgtcga tctcgacgct gcgccaaccc atcccggcaa tccactacat
     2401 ggtggtgctc gccggctccg acgaaatccc gtgcgccgac tacgcgacgt tcggcagcac
     2461 gcagctcgcc ctcaacgccg tcaccgcgct gcgcggtggc aacgcatgcc tgctcgcaaa
     2521 tcacggcatg gtcgcactcg gcaagacgct gcccgaagcg ctgcggctcg cagccgaagt
     2581 cgagacgctc gccggtcagt actggcacgc cgcacaaatc ggcacgccct acatcctcga
     2641 cggcgatgag ctgcagcgcg tgcgcgcgaa gttcgccgag tacggccaga agaagccgcg
     2701 gcgctactga ctctcgagct cgcgaatctt gcggtcgatg cgcgtcatcg agatcgaccg
     2761 aacgacgaac atgatcgctg cgcccgcact ctgcgagatc atgaagagcc acagcacgag
     2821 atcgagccgg tcgatcagga gcgcgagacc gagccagaac cagaggtccg cctcttcgaa
     2881 cacgaagacg accgcgacct tcttgagaaa ccacagcgcc cagtccttcg cggtgcgcac
     2941 gggcggcggc ttgatcacga tcggagccgt gcgcttcgcg accgcggcaa ccgggtcggc
     3001 ctttgcttcg agccagcgca ggcgctcgcc ttcggccact tcgagccact tcgtgtagcc
     3061 gcgcaggttc agcgtgacga cggaaatcgc ggcaaacaac gtccacttgt tgtcgccggt
     3121 cagctcggtc tctttccagc cgaccgccat catgatgacg gtccacgtga ccatgtcgga
     3181 cgccttgtcg tagaaggagc cgaacttcgt gaagacgcgg cggtaccgcg cgatcgtgcc
     3241 gtcgaggtgg tcgaagatga ggccgacctg caggaggagc gccgccgcga gccagtgctg
     3301 cttgtcgagg atcagccacg ccgccgcgag cttgctgaga tttgccagcg tcgtgacacg
     3361 attcggtgtg agcggccgcc agtcggcaat gaccagcatg atcaggatcg tgagcggccg
     3421 aatgatcagc gccggatacc agtcttcgct ctggaagtta cgtatccgct gcagccgctc
     3481 gcgattcaca gcgccggcaa tatcgcacgt tgcttgcgaa gccgtgacgt ccgcaatacg
     3541 gagatcgctg tagtctggtc ccgttagatg tccgcgcagc agcgaatgaa taccaacgtc
     3601 aaccgcggtc tcgcgtggct tggcgttgcg agctcactcg tcggtctgct cgacatcgtc
     3661 tccatcctga tcatcctcaa caactggatc tcagcggagg agtacggaat cgcgacgaag
     3721 tgcatctgga tcttcccggt gctcgaccaa gcgaccgacc tcgggctctc ggcggccgtg
     3781 gttcaacgcg acgaccacac gccgcggcgc atctcgacgg tcttttggat caacgtgatg
     3841 ctgtccctcg cgttgttcgc gctgattgcg atcaccgcac cgatcctcgc ggtgaatttc
     3901 tacggccacg caattgttgg ctggatgctc atcgcgtacg gcggaaagct catcctccag
     3961 aacggctact tcatcccatg ggcgatgatg aagcgcgagc tgcggttcaa ggagctctcg
     4021 gctttgcgga tcagcgcgaa tgtcgtcgag ttcatcgcga agatcggatt tgcggcgacg
     4081 gggttcggca tctggtgctt cgtgctcgca ccgctgttgc gaacggttac gtacctgatc
     4141 ggtacgcagc tccgtcatcg gtatcgaccg cgctgggagt tctcgtatcc cgaagcgaag
     4201 gagtacgtga ccttcgggct gcgcagctcg ggaagtcaga tcctgttctt cttctactcg
     4261 aacgtggact atccgatcgt cggttacttc tttggcgacg tcgcgcttgg cttctatcgg
     4321 ctcgcctacg agatcgtgct cgagcccgtt cgcgtgattt cgaatgttgt cgtcgatgtc
     4381 gcgttcccgg ctttcgcgaa gctccgggcg aacaccgccg cattggtcga tcagttcgtc
     4441 tcgttcacgc ggctgaacct catcaccgtg atgagctact cagcgatcgt cttcgtggcg
     4501 gccccggacg tcctcgccct gttgttcccg tcctacaagg gctcgacgga agccgtgcag
     4561 atcttgtgtg ccgttgccgt cctgcgcgcg gttggttttg tcgcgccgcc actgctcgac
     4621 ggtgtcgggc atcccgagcg gacactgcgc tacatgacgg cagccgcggt ggcgatgccg
     4681 ctcgcattca cactcggtgc gattctcctc ggcccgcgcc ttggcttcga gtcggtggcg
     4741 gccgcgtggg cgctcggtta tccgattgca ttcgccgtcc tcatctatat gacggtcgtg
     4801 accctcaact ggtcggtcgc gaagtacgtg cgcgcggtgg gcggcgtggc cctgtgcatg
     4861 ctcgcggcgg gcggcgtggc agctgtcgtc tatccgctgc ttcccgcggg tcatggcgtg
     4921 cgggcgctcg ttacgacggt cgtcgtcgtg gtagtcagtg cgttgctgct cgcctatacg
     4981 caggggctca cgttgcgcgg cgtcgtgcgg tcgatgcgcg aaccgccgcc ttaaaccgcg
     5041 gcgcgacgca ggctcgtgcg agcggcacgt tcgtgcagcg ccttcacgac gtcgggtcgc
     5101 g
//
LOCUS       DATLBZ010000582         4187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7127894, whole genome shotgun sequence.
ACCESSION   DATLBZ010000582 DATLBZ010000000
VERSION     DATLBZ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7127894"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VMZ53_28775"
     CDS             complement(<1..293)
                     /locus_tag="VMZ53_28775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32543.1"
                     /translation="MRCLVLVLLVACDAGKKPVTSPPASSSSGTAANLADATAAPQSA
                     IDAAVVDPAVVDAAIVEATPDAAVDAPADIRITWLVYPPSVPVKNMPEMLQQPV"
     gene            complement(332..1939)
                     /locus_tag="VMZ53_28780"
     CDS             complement(332..1939)
                     /locus_tag="VMZ53_28780"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HUS32544.1"
                     /translation="MALLGSCTVGAPPGFSNGDRWSVPLIGPLEDGLLLVPALVNDKG
                     PYVFAIDPDAHVSIIDQDVVKETGARIGEGPHLLDETDTQQPRYFAEILRWDLGTLSV
                     QGPKSAQIVPAGTFDAAGRRIHGVIGRDIIADSLVFSFDRDRGVVTLQTTKSAKAPAN
                     AIPIKYSVLRSRIENAEVLPISRRLVKANVNGLPFTMHVDFGAMPSQLRPRSWAKAKL
                     AESDLQIALVDEVGMSRDIKKQGVAQAVQLGPITANDVSFVPYVDKRWPDEDLEGTLG
                     LHFFRRVSVMANWDSQMIYMWPRNDVTRDVVARMSRWQSKTLTSCEHPGCVKVTLIDP
                     LANKQPPAPPPADGTAAPATDGSATPPADGAAPSRAPSPPAPSAPPAPAPSPAPSAPP
                     PPAPAAPPPAAPARAPSPPAPSAPPPPAPSPAPSAPPPPSAPPPPAPAVTSHPGLVAS
                     IVRDPSSIDLPLEVLVAVTPAQGKPPLKWIVVNLPSGVDRAMTHLSADYLGATLTVLD
                     ASPFPRACPTDGSCVDMLAPPQVIAQP"
     gene            complement(1957..2982)
                     /locus_tag="VMZ53_28785"
     CDS             complement(1957..2982)
                     /locus_tag="VMZ53_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HUS32545.1"
                     /translation="MSEAVKLKLSKKDLESDQDVRWCPGCGDYAILATVQRTLANLGV
                     KRENTVFISGIGCSSRFPYYMNTYGFHTIHGRAPAVASGLKITRPELDVWVITGDGDG
                     LSIGGNHMIHALRRNMDLKILLFNNQIYGLTKGQYSPTSAMGTRAPSTPSGAIDYPLN
                     PISLALSAGATFIARAADTDAKLMGKILEEAYKHKGAVFIELLQNCPVFNDGVWEGVK
                     DDPSNKQIALVDGKPLTFAGGTKGIKLGAGLVPEIVDVKDGDTSQLLVHSERGSTLYA
                     NALSQLVQPNFPMPMGILYRTDKPSYDQLAQAQVTEAIEKQGTGDLTKLLYSGMTWEV
                     GADGVRH"
     gene            complement(2982..>4187)
                     /locus_tag="VMZ53_28790"
     CDS             complement(2982..>4187)
                     /locus_tag="VMZ53_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HUS32546.1"
                     /translation="PATFAPGMYRNITGNEAIALGLVVGAELAGKKLFYSGYPITPAS
                     SILHSLAKYKNFGTLTFQAEDEIAAMGAALGAAYGGSIAVTASSGPGIALKGEAMGLG
                     VMTELPVVILNIQRGGPSTGLPTKTEQSDLLQSLYGRNGESPLPVLAAKSPGDTFYCA
                     IEAVKIATRYMVPVILLSDGYIANGSEPWPLPNLTALPRFDVIHRTDPTGYFVYQRDE
                     RTLARSWVIPGTPGLEHRIGGIEKDALTGNISYAPENHEKQTLIRQEKVDRIAQDIGE
                     LDLAGGESGDVLLIGWGGTYGALRQAQGALTAQGKKVSHAHLRWLSPLEPGLAKIIHN
                     FKTVIVAELNMGQLRTVIRDKFLVDAHGLNKIQGQPFKVREVMVAVDKQLESLPASAS
                     SSSHIQVNA"
BASE COUNT          665 a         1278 c         1513 g          731 t
ORIGIN      
        1 accggctgct gcagcatctc cggcatgttc ttcaccggca cgctcggcgg atacacgagc
       61 catgtgatcc ggatgtcggc cggtgcgtcg acggcagcat cgggtgtcgc ctcgacaatc
      121 gccgcatcga caaccgcggg atcgacaacc gccgcatcaa tggcgctttg tggtgcggcg
      181 gtcgcgtcgg cgagatttgc cgccgtgccg ctcgacgatg acgcgggtgg cgatgtcaca
      241 ggcttcttgc ccgcgtcaca tgccacgagc aatacgagca ccaggcagcg catccaagcg
      301 aggtaacgca ccacgctcgg cgcctcttcc atcatggttg ggcaatcacc tgcggcggcg
      361 ccagcatgtc gacgcagctg ccatctgtcg ggcaggcgcg cgggaatggg ctcgcgtcga
      421 ggacggtgag cgtcgcgccg aggtaatcgg cggaaaggtg cgtcatcgcg cgatcgacgc
      481 ccgacggcag attcacgacg atccacttga gcggaggctt gccttgcgcc ggcgtgaccg
      541 caacgagcac ttcgagcggc agatcgatcg acgatggatc acgcacgatc gatgcgacga
      601 gacccggatg cgatgtcact gcgggggcgg gcggtggggg cgcggaaggc ggtggcggcg
      661 cggaaggcgc tggcgatgga gcgggcggtg gcggcgcgga aggcgctggc ggcgatggag
      721 cgcgtgcggg cgcagcaggt ggcggcgcgg cgggggcagg cggtggcggc gcggaaggcg
      781 ctggcgatgg agcgggcgct ggcggcgcgg aaggcgctgg cggcgatgga gcgcgtgagg
      841 gcgcagcacc gtctgcgggc ggcgtggcgg agccgtctgt ggcgggcgcg gcggtgccgt
      901 ctgcgggcgg cggcgcgggc ggctgcttgt tcgcgagcgg atcgatcagc gtgaccttca
      961 cgcagccggg gtgctcgcag ctcgtgagcg tcttcgactg ccagcgcgac atccgcgcga
     1021 cgacatcgcg ggtcacgtcg ttccgcggcc acatgtagat catctgcgag tcccagttcg
     1081 ccatcaccga gacgcgccgg aagaagtgca ggccgagcgt gccttccaga tcctcgtcgg
     1141 gccaccgctt gtcgacgtac ggcacgaacg agacatcgtt ggccgtgatt gggccgagct
     1201 ggacagcttg cgcgacgcct tgcttcttga tgtcgcgcga catgccgact tcatcgacga
     1261 gcgcgatctg cagatcggat tcggcgagct tcgcctttgc ccacgagcgc ggacgcagct
     1321 gcgacggcat cgccccgaag tcgacgtgca tcgtgaacgg gaggccgttg acgttcgcct
     1381 tcacgaggcg gcgcgagatc ggcagcacct cagcgttctc gatccggctg cggagtaccg
     1441 agtacttgat cgggatagcg ttcgccggcg ccttcgccga ctttgtcgtc tggagcgtga
     1501 cgacgccccg atcgcgatcg aacgagaaca cgagcgagtc ggcgatgatg tcgcgaccga
     1561 tcacgccgtg gatgcggcgg ccggcggcgt cgaacgttcc tgcggggacg atttgcgcgg
     1621 acttcggacc ttggaccgag agcgtgccga ggtcccagcg caggatctcg gcgaagtagc
     1681 gcggctgctg cgtgtccgtc tcgtcgagga ggtgggggcc ttcgccgatg cgcgcgcccg
     1741 tctctttgac gacgtcctga tcgatgatcg agacgtgtgc gtcgggatcg atcgcgaaga
     1801 cgtacgggcc cttgtcgttg acgagcgcgg gaacgaggag cagaccgtct tcgagcgggc
     1861 caatgagcgg cacgctccag cgatcgccgt tggagaagcc cggcggcgca ccgacggtgc
     1921 agctaccgag cagagccacc gcagcgagag ccgctgctag tgacgaacgc cgtctgcgcc
     1981 gacttcccac gtcatgcccg agtacagcaa tttggtgagg tcgcccgtgc cttgcttctc
     2041 gatcgcttcg gtcacctgcg cttgcgcgag ctgatcgtac gacggcttgt cggtgcggta
     2101 gaggatgccc attggcatgg ggaagttcgg ctgaacgagt tgcgagagcg cgttggcgta
     2161 gagcgtcgag ccgcgctcgc tgtgcacgag cagctgcgag gtatcgccgt ctttgacgtc
     2221 gacgatttcg gggacgagac ccgcgccgag cttgatgcct ttggtgccgc ccgcgaacgt
     2281 cagcggcttg ccatcgacga gcgcgatctg cttgttcgag gggtcgtctt tgacgccttc
     2341 ccacacgccg tcgttgaaga cggggcagtt ctggagcagc tcgatgaaga ccgcgccttt
     2401 gtgcttgtag gcctcttcga ggatcttgcc catcagcttc gcgtcggtgt ctgccgcgcg
     2461 cgcgatgaac gtggcgccgg cggacagcgc gagcgagatc gggttgagcg ggtagtcgat
     2521 cgcgcccgat ggcgtcgaag gagcgcgcgt acccatcgcc gacgtcggcg agtactggcc
     2581 tttcgtgagg ccgtagatct ggttgttgaa cagcaggatc ttgagatcca tgttgcgccg
     2641 gagcgcgtgg atcatgtgat tgccgccgat cgacaggcca tcgccatcgc cggtgatgac
     2701 ccacacgtcg agctccggac gcgtgatctt gagacccgac gcaaccgccg gcgcacggcc
     2761 gtggatcgtg tggaagccgt acgtgttcat gtagtacggg aagcggctcg agcagccgat
     2821 gcccgagatg aagacggtgt tctcgcgctt cacgccgagg ttcgcgagcg tgcgctggac
     2881 cgtcgcgagg atcgcgtagt caccgcagcc cgggcaccag cggacgtcct ggtccgattc
     2941 caggtccttc ttcgagagct tgagcttgac tgcttccgac attaggcatt cacctggatg
     3001 tgagaggacg aagacgcgct ggcaggcagc gactcgagtt gcttgtcgac tgccaccatc
     3061 acctcgcgga ccttgaacgg ctggccctgg atcttgttga ggccgtgcgc gtcgacgagg
     3121 aacttgtcgc ggatgaccgt gcgcagctgg cccatgttga gctcggcgac gatgacggtc
     3181 ttgaagttgt ggatgatctt cgcgaggccc ggctcgagcg gcgagagcca gcgcaggtgc
     3241 gcatggctga ccttcttgcc ttgggccgtg agcgcgcctt gtgcctggcg aagcgcaccg
     3301 taggtgccgc cccaaccgat gaggaggacg tcgcccgact cgccgccggc gagatcgagc
     3361 tcgccgatgt cttgcgcgat gcgatcaact ttctcctggc gaatcagcgt ctgcttctcg
     3421 tggttctccg gcgcgtacga gatgttgccg gtgagcgcgt ctttctcgat gccgccgatg
     3481 cggtgctcga ggccgggcgt gcccgggatg acccacgagc gagcgagcgt gcgctcgtcg
     3541 cgctgataga cgaagtagcc cgtcgggtcg gtgcggtgaa tgacgtcgaa gcgcggcagc
     3601 gccgtgaggt tcggcagcgg ccacggctcc gagccgttcg cgatgtagcc gtcggacagg
     3661 aggatgaccg gcaccatgta gcgcgtcgcg atcttcacgg cctcgatggc gcagtagaag
     3721 gtgtcgccgg gcgacttcgc ggcgagcacc ggcagcggcg attcgccgtt gcggccatag
     3781 agcgattgca ggagatccga ttgctcggtc ttcgtcggca ggcccgtcga cggaccaccg
     3841 cgctggatgt tgaggatcac gaccggaagc tcggtcatga cgccgaggcc catcgcttcg
     3901 cccttgagcg cgatgcccgg gcccgacgat gcggtgaccg cgatcgagcc gccgtacgca
     3961 gcgccgagtg cagcgcccat cgcggcgatc tcgtcttcgg cctggaacgt cagcgtgccg
     4021 aagttcttgt acttcgcgag cgagtgcaaa atcgacgacg cgggcgtgat cggatagccc
     4081 gagtagaaca gcttcttgcc ggcgagctcc gcaccgacga cgaggccgag cgcgatcgct
     4141 tcgttgcccg tgatgttgcg atacatgccc ggcgcgaacg tcgcggg
//
LOCUS       DATLBZ010000583        11624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7130579, whole genome shotgun sequence.
ACCESSION   DATLBZ010000583 DATLBZ010000000
VERSION     DATLBZ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7130579"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VMZ53_28795"
     CDS             <1..252
                     /locus_tag="VMZ53_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HUS32547.1"
                     /translation="YNGIVKKIVEIGAFVEIMPGTDGLLHISEVAKERIRSVEDVLSE
                     GDEVVVKCIKVDRDGKIRLSRREALDANPGPEEVHNFVI"
     gene            455..1339
                     /locus_tag="VMZ53_28800"
     CDS             455..1339
                     /locus_tag="VMZ53_28800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HUS32548.1"
                     /translation="MVLLLGACTLDESPTEIEIATPDEPLPVDLRAGDVVRLQDLALE
                     VPAAGEAVTLFADGADGSHVELTLVNRDGIVELLSDDAAELEPVIIAASSTAACHDGA
                     FHLSGHKWTTEYQWMFAAGTTPSANSRDNVETALVHAANAIVNSRNDCGLADIVSATT
                     RYLGRTASKPNIPATTSTVSCGTRDNTSSVGFGGLPTTYLGVACTWTDGNGHALEGDV
                     KLSTRHAWFALDVPASCSNRFGVQAVATHEFGHVFGLGHVSEANHPKLTMSTAAAACS
                     NAPYTLGLGDVRALRQLY"
     gene            1353..2294
                     /locus_tag="VMZ53_28805"
     CDS             1353..2294
                     /locus_tag="VMZ53_28805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32549.1"
                     /translation="MRRWWVAMMLAACGDNVDSQATGIWGANLAVADDTIFFTTYAEG
                     PDKTDHAVWARPLVGGPDRKLWQGAPDGAFGTGMAVFDGRVYWPAWEGISEGEMPIIA
                     SAPSDGGERTIDGALPSRNEVSGGVIVDATGIYAAQSDAVTRLDRGGGGTTVLHSGTP
                     GIRWIARDSGSLYVTTIEQNPRLLRLGAVSQELIASGQYTGTFAIADKVAYLGTNGAI
                     VRVSLDGTTTGLTPLVVDGDAQIVVAGAAGVFAVVTNRALGVNHDQIVRVAVDGSSPE
                     VLRDGLEYVFALVLADNHLVFAQCDCSAPGLIGVIEL"
     gene            2302..2745
                     /gene="dut"
                     /locus_tag="VMZ53_28810"
     CDS             2302..2745
                     /gene="dut"
                     /locus_tag="VMZ53_28810"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HUS32550.1"
                     /translation="MTTPRVQIRRLRPDAMLPRYQTADAAGMDLHAALDAPMQVAPGA
                     VVAVPTGLAMAIPRGFEGQVRPRSGLAAKHAVTVVNAPGTVDADFRGTVMVLLINHGR
                     EPFMINHGDRVAQLVIAPVSQAEIVEVSELDDTQRGAGGFGSTGR"
     gene            2750..3178
                     /locus_tag="VMZ53_28815"
     CDS             2750..3178
                     /locus_tag="VMZ53_28815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32551.1"
                     /translation="MAARSKTPTKRGTTPPSTDEIPTEILGEDFARRAKAKTAKKPAE
                     QIPSPPVLPAAPVIKAISMKTPGDVQKREMPESEMPHVKLRAMSEMSRAQTPQNLGNL
                     APPYDPSEARKRSVREYVVWGCIAIMIACAIALVVWFVAR"
     gene            complement(3180..3905)
                     /locus_tag="VMZ53_28820"
     CDS             complement(3180..3905)
                     /locus_tag="VMZ53_28820"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HUS32552.1"
                     /translation="MRSALVFGLLGCGRIGFAPIDDRVGDAAATDDAAMIDGRPIDAP
                     ANAVTVSFGDRGDATFQATASDTYISNEAGEPTLNYGFSDELRSEQDVDERILLRFDV
                     DTIPPTATVFQVQLSITVTEANTVATWFVHPVLEQWSEGTADGTAGAANFTQRMPGTA
                     WAVVGAGMPGSSGLAFATIKPNALGTINATLPNAPVQNWVTNPATNFGMIFLNDDTNT
                     ARFAASENATPAMRPLLTVTYVP"
     gene            complement(3930..5537)
                     /locus_tag="VMZ53_28825"
     CDS             complement(3930..5537)
                     /locus_tag="VMZ53_28825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32553.1"
                     /translation="MRWGRLLVGGLLIATGCKSSSNGNQTPDADADVLASTCGDGTLD
                     PNETCDPPDSCPAACADGDVCTKDVIAGSAASCNVACASTPITTCGGQDGCCPSGCDS
                     NSDADCPPPAGTALKDRLTVTTTTTPAGALAAGSAYRIWNASNGQQSLKVMPVFTVPF
                     ADCGTLVGYTTGTAAAPRARVARLDAGDALVTTYDLGQYVLRGLAAEPDGHFAALLWD
                     ISTTPKSLKLIRYASNGAAGTTATLPTTNVAPTDFSIGESRLEYDVPNNRYGAYFHVH
                     GTNPSYFAYGHEGDMLAWVDKSAGTVSLGWEWGCSHSMSELLRYSPAANATLPVCVTD
                     CYPGTTGSNFATGSIGGLYLNHDQTKVRDFDGGCNGSVAAELGGAAPGVAGWKLVFNG
                     HQNAAAMGQSSYSTSTMNQDIGFISVANNKSLSGSIVWLTTTTANEANSTIARWQPMG
                     GTGEQYVVGWSAGSTFNLARITDTGTIVEGPTAITTAKWGERDDPFRTHLNSDIVWAW
                     FDSAGSTTLKLARLRSGGTAASCAALP"
     gene            5597..6955
                     /gene="purB"
                     /locus_tag="VMZ53_28830"
     CDS             5597..6955
                     /gene="purB"
                     /locus_tag="VMZ53_28830"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HUS32554.1"
                     /translation="MIPRYSRPELAQLWEDKTRFELWLDIELAACEAMEKRGTVPAGT
                     AAKVREAAKGKLDPARILEIEATTRHDVIAFLTHVEELAGEPARWLHLGMTSSDVLDS
                     CLAIQTGRALEKIVQGVMGLAQALADRAREHAKTPTIGRSHGIHAEPITAGLVFARWH
                     AEVVRASSRIIRARHAIAVGKIAGAVGVYGNLDPEIEREALGALSLEPETVATQIVAR
                     DRHAEVFCALALLGTAIEQIALGVRHWQRTEVGEAEEGFTPGQKGSSAMPHKKNPILS
                     ENLTGLARLLRSYAGAALEDVALWHERDISHSSVERVAMPDATILADFMTARCTNLVK
                     NLVVNPRRMKANLDRTGELFFSEAVLLALVKTGLPRQEAYVMVQRNALKAISEASSMG
                     HVGALPGRFRELLGQDADIKQRLESKALDACFDLEHHLRHAPEILERALGPAPRNDGG
                     SN"
     gene            6952..7938
                     /locus_tag="VMZ53_28835"
     CDS             6952..7938
                     /locus_tag="VMZ53_28835"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HUS32555.1"
                     /translation="MTTLDDQLRAQLDQTLVETPFRSLGGRTLPRYDGKVRDCFIDKD
                     RGERVIIVTDRLSAFDAVVGTIPFKGQVLNQLAQFWFEQTAALAPNHMLRVPDPNVMI
                     ARECDPLPVELVMRSYLTGVTSTSIWKAYEQGKRTFCGHALPEGMKKNEKLPKAILTP
                     STKAAKGDHDISVSRDELLAMGKIEPAVFDRAAAIAEKLFAAGQAHAAKRGLILADTK
                     YEMGLDRDGNIIIIDEIHTPDSSRYWYADDYEARLAKGEEPRSLDKEYVRRWLAGEAK
                     WSGDGPPPVMPPDIRMEAARRYIASYELVTGKQFMPDTREPIARIAAALEAK"
     gene            8020..>11624
                     /gene="purL"
                     /locus_tag="VMZ53_28840"
                     /gene_synonym="purI"
     CDS             8020..>11624
                     /gene="purL"
                     /locus_tag="VMZ53_28840"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HUS32556.1"
                     /translation="MTAPQLHIWPGRPALSAARKAATLARIRAVSPDVTSVEARWMHI
                     ITCGRELTAQETEQLAQMLSYGPAEPPQKSNRRLETVETVSFVITPRVGTVSPWSSKA
                     TDIAHVCGLTAVTRIERGIEWTMVGDSIEVPVIAALLSDRMTESVIMREQDLAKVVAP
                     AGEPRPLQFVRLGERGADTLREASQKMGLALAEDEIEYLVTRYRELGRDPTDVELMMF
                     AQANSEHCRHKIFNADWFISEEKQERSLFQWIKESTKNAPTGVLSAYKDNAAVVEGNV
                     ADRFFPDPDGVYRAHREPSHILGKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGA
                     KPKAGLVGFTVSDLRIPDALEPWEPRGEQWMGSPRRIASALDIMIDGPLGGAAFNNEF
                     GRPAILGYFRTFELGDRGYHKPVMLAGGIGAIRPHHVQKAAVPAGAALVVLGGPAFLI
                     GLGGGAASSLAQGASAEDLDFASVQRDNAEVQRRCQEVIDRCWAAGEANPILSIHDVG
                     AGGLSNAMPELVNDAGLGAKLDLRKIPTGEPDLSPLELWCNESQERYVLAIAPDRIEE
                     FAALCARERAPWAQLGVATPPVPGVEGSPQSGRLTVADVKGGPPPVDLPLELVLGKPP
                     RMTRKAEPLPSPRRPLDFAGASVAEALDRVLGLPTVADKSFLVTIGDRTVGGLVTRDP
                     MIGRFQVPVADCALTTAGFDTYAGEVMSIGERPPVALLDAAAASRLAIGEAITNLAAA
                     PIGIAGRAPGEPLGPGDLSSIKLSCNWMAAAGHPGEDARLYEAVRAAGETAVALGISI
                     PVGKDSMSMRTQWAGQGDERRVIASPVTLVATAFGPVTDVRSNVTPELRGTGTLLFVD
                     LGNGKNRLGGSCLAQVYGQLGDAPPDLDEPARLRSFFTVMQSLVAAGTLLAYHDRSDG
                     GLIVAALEMAWAGGLGLELDVTNVDADPFRALFSEELGALLEVASADVPAVRAAFEAH
                     GIAVQPIGRGVEGNRVVVLHGEATKVIDESRFTLRARWSHVTREIARRRDDADCASEE
                     HEARVERTNIGLTAEVRFDPNQDIAAPFIATGVRPRVAILREQGVNGQIEMAAAFSRA
                     GFDAIDVHMTDLIEGRVDLSDCRGAVACGGFSFGDVLGAGRGWAATFRYNARAQSAIR
                     RFVDRADTFLLGVCNGCQMVADLAPDLVESTRDVWPRFVKNRSERFEARVVMLRI"
BASE COUNT         1923 a         3836 c         3950 g         1915 t
ORIGIN      
        1 tacaacggca tcgtgaagaa gatcgtcgag atcggtgcgt tcgtcgagat catgcccggc
       61 accgacggcc tcctccacat ctcggaagtc gcgaaggagc gcatccgctc cgtcgaggac
      121 gttctctccg agggtgatga agtcgtcgtg aagtgcatca aggtcgatcg cgacggcaag
      181 atccgtctgt cgcgccgcga agcactcgac gcgaacccgg gcccggaaga agttcacaac
      241 ttcgtcatct aagacgagcg tcgtgaaacg aatgcagggt gcggttcgcc gcatcctgtt
      301 tgcgtttcgg catgaccgca tcggctaggc tgcaacctcg atcgtgcatg tgtcgggctg
      361 tccccgcagg cggcgatcga actgcgtgaa accacggtgg cgcgagctgg ccacatcctt
      421 gctgttcccg cgcgcgatgc gccgtattgc tttcgtggtc ctgctcctgg gtgcgtgcac
      481 gctcgacgag tcgccgaccg aaatcgagat tgcaacgccc gacgagccac ttcccgtcga
      541 tctgcgcgcc ggtgacgtcg tccgcctgca ggacctcgcg ctcgaagtcc ccgccgccgg
      601 ggaagccgtc acgctgttcg ccgacggagc tgatggctcg cacgtcgagc tcacgctcgt
      661 gaatcgcgat ggcattgtcg agctcctcag cgacgacgca gccgagctcg agcctgtcat
      721 catcgccgcg agctcgaccg ctgcgtgcca cgacggcgcg tttcatctct ccggccacaa
      781 gtggacaacg gagtaccagt ggatgttcgc cgcaggcacg acgccgtcgg ccaacagccg
      841 agacaacgtc gagacggcgc tcgtccacgc ggcaaatgcg atcgtgaatt cgcgcaacga
      901 ctgtggtctc gccgacatcg tctcggcgac gacgcgctat ctcggccgca cggcatcgaa
      961 gccgaacatc cccgcgacga cgtcgacggt ctcgtgcggc acgcgtgaca acacgagctc
     1021 ggtcggcttc ggcggcctgc ccacgacgta cctcggcgtg gcgtgcacgt ggaccgacgg
     1081 caacggccat gcactcgaag gcgacgtcaa gctgtcgacg cgtcacgcgt ggttcgcact
     1141 cgacgtgccc gcgagctgct cgaatcgctt cggcgtccaa gcggtcgcga cgcatgaatt
     1201 cggtcatgtc ttcggcctcg gccacgtgtc ggaggcaaac catccgaagc tgacgatgag
     1261 cacggcggcg gccgcgtgca gcaatgcgcc gtacacactc ggccttggtg acgttcgcgc
     1321 gctgcgccag ctctactgag gtacgatgag gcgtgcgtcg atggtgggtg gcgatgatgc
     1381 tcgcggcgtg tggcgataac gtcgatagcc aggcgacggg aatttggggt gcgaacctcg
     1441 cggtcgccga cgacacgatc ttcttcacga cgtatgccga aggccccgac aagaccgacc
     1501 acgccgtgtg ggcacgaccg ctcgttggtg ggcccgatcg caagctctgg caaggtgcgc
     1561 cggacggcgc tttcggtacg ggcatggcgg tcttcgacgg acgcgtgtac tggccggcgt
     1621 gggagggcat cagcgaaggc gagatgccca ttatcgcgag cgcgccgagc gacggtggcg
     1681 agcgcacgat cgacggtgcg ttgccgtcgc gcaacgaggt gtccggtggt gtcatcgtcg
     1741 acgcgacggg tatctacgcc gcgcagtctg atgccgtcac ccggctcgac cgcggcggcg
     1801 gtggcacgac ggtgttgcac tcgggtacgc cgggtatccg atggatcgcg cgcgatagcg
     1861 gctctctgta cgtgaccacg atcgagcaga acccgcggct gctccggctc ggagccgtct
     1921 cgcaggagct gattgcgagc ggccaataca ccggaacgtt cgcgattgcc gacaaggtcg
     1981 cgtacctcgg cacgaacggc gcgatcgtgc gggtctcgct ggacgggacc accaccgggt
     2041 tgacgccgct cgtcgtcgac ggcgatgcac agatcgtggt tgcgggagct gccggcgtgt
     2101 ttgccgtcgt gacgaaccgc gcgctcggcg tcaatcacga ccagatcgtg cgcgtcgcag
     2161 tggacggcag ctcgccggag gtcctgcgcg atggtctcga gtacgtcttt gcgctcgtgc
     2221 tcgccgacaa ccacctggtg ttcgcgcaat gtgattgctc ggcgccgggc ctcatcgggg
     2281 tgatcgagct atgatggcgg cgtgacgaca ccccgcgtgc agattcgccg gctccggccc
     2341 gatgcgatgc tgccgcgcta ccagaccgcc gatgccgccg gtatggatct gcacgccgcg
     2401 ctcgacgcgc cgatgcaggt ggcgccaggc gcggtggtcg cggtgccgac cggacttgcg
     2461 atggcgattc cgcgcggctt cgaagggcaa gtccgaccgc gctcgggtct cgctgcgaag
     2521 cacgcggtca cggtcgtcaa cgcgcccggc acggtcgacg cagacttcag aggcaccgtg
     2581 atggttctgt tgatcaacca cggtcgcgag ccattcatga tcaatcacgg tgatcgcgta
     2641 gcgcagctcg tgatcgcgcc cgtatctcag gcagagatcg tcgaggtcag cgagctcgac
     2701 gacacgcagc gcggcgccgg cggcttcggc tcgacggggc gatagcaaga tggccgcgcg
     2761 gtcgaagact ccgacgaaac gaggcacgac gcctccgtcg accgacgaga tacccacgga
     2821 gatcctcggc gaggacttcg cgcgccgcgc caaggcgaag accgcgaaga agcccgcaga
     2881 gcagattcca tcgcctcccg tattgcccgc ggcgcccgtg atcaaagcga tctcgatgaa
     2941 gacgccgggc gacgtgcaga agcgcgagat gccggagtca gagatgccgc acgtcaagct
     3001 tcgtgcgatg tccgagatgt cccgcgcgca gacgccgcag aatctcggca acctcgcgcc
     3061 gccatacgat ccgtcggaag cgcgcaaacg cagcgtgcgc gagtacgtcg tgtggggctg
     3121 catcgcgatc atgatcgcgt gcgcgatcgc gctcgtcgtc tggttcgtcg cgcgctagct
     3181 cacggaacgt aggtcacggt cagcagcggg cgcatggccg gcgtcgcgtt ctcactcgcc
     3241 gcgaaccgtg ccgtgttcgt gtcgtcgttg agaaagatca tgccgaagtt ggtcgctgga
     3301 tttgtgaccc agttttgaac gggcgcgttc ggcagggtcg cgttgatcgt gccgagcgcg
     3361 ttcggtttga tcgtcgcgaa ggccaggccc gacgagccag gcatgcctgc acctacgaca
     3421 gcccacgcgg tgcccggcat tcgctgcgtg aagtttgcgg cgcccgcggt gccgtccgcc
     3481 gttccttcgc tccattgttc gagcactggg tggacgaacc acgtggcgac ggtgtttgcc
     3541 tcggtcaccg tgatcgagag ctggacctgg aagacagtcg cggtcggcgg aatcgtgtcg
     3601 acgtcgaacc gcagcaggat ccgctcgtcg acgtcttgtt cggagcgcag ctcgtcggaa
     3661 aagccgtagt tgagcgtcgg ctcgccggct tcgttgctga tgtacgtatc gctggccgtg
     3721 gcttggaacg tcgcgtcgcc gcgatcgccg aacgagacag tgacggcgtt cgcgggtgcg
     3781 tcgatcgggc gcccgtcgat catcgcggcg tcgtcggtgg cggcggcatc gccgacgcga
     3841 tcatcgatcg gcgcgaatcc gatgcggccg catccgagca ggccgaaaac caacgcacta
     3901 cgcatctgca acgattgtag agcgagtcgc tacggcaatg cagcgcacga cgccgcggtt
     3961 ccgccggagc gcagtcgcgc gagctttagc gtcgtcgagc cggcgctgtc gaaccacgcc
     4021 cacacgatgt cgctgttgag gtgcgtgcgg aacggatcgt cgcgctcgcc ccatttggcc
     4081 gtcgtgatcg cagtcgggcc ttcgacgatc gtgcccgtgt cggtgatgcg cgcgaggttg
     4141 aacgtcgagc cggcggacca gccgacgaca tactgctcgc cggtgccacc catcggctgc
     4201 cagcgcgcga tcgtcgagtt cgcctcgttg gcggtggtcg tcgtgagcca cacgatcgag
     4261 cccgagagcg atttgttgtt cgcgaccgag atgaagccga tgtcctgatt catcgtcgac
     4321 gtcgagtacg acgactgccc catcgccgca gcgttctggt gcccattgaa gacgagcttc
     4381 cagcccgcga caccgggcgc cgcaccgccg agctctgccg cgacgctgcc gttgcagccg
     4441 ccgtcgaagt cgcgcacctt cgtttgatcg tggttgaggt agagaccgcc gatcgagccg
     4501 gtcgcgaagt tcgagcccgt cgtgcctgga tagcagtcgg tcacgcacac cggtagcgtg
     4561 gcgtttgcgg cgggcgagta gcggagcagc tcactcatcg aatgcgagca gccccactcc
     4621 cagccgagcg acaccgtgcc cgcgctcttg tcgacccacg cgagcatgtc gccttcgtgg
     4681 ccgtacgcga agtagctcgg gttcgtgcca tggacgtgga agtacgcgcc gtagcgattg
     4741 ttcgggacgt cgtactcgag gcgcgattcg ccgatcgaga agtcggtcgg cgcgacgttc
     4801 gtcgtcggca gcgtcgccgt cgtgccggca gcgccattcg atgcgtagcg gatgagcttg
     4861 agcgacttgg gcgtcgtcga gatgtcccac agcagcgctg cgaagtgacc gtcgggttcg
     4921 gcggcgaggc cgcggagcac gtactggccg aggtcgtacg tcgtgacgag cgcatcgccc
     4981 gcatcgagcc gcgccacacg tgcgcgaggt gctgccgctg ttcccgtcgt gtagccgacg
     5041 agcgtgccgc aatcggcgaa cgggaccgtg aagactggca tcactttgag tgactgctga
     5101 ccgttcgacg cgttccagat ccggtacgcg ctgccagccg cgagggcgcc cgcgggcgtc
     5161 gtcgtcgtgg tgacggtgag acgatctttg agcgcggtgc cggccggcgg tgggcagtcg
     5221 gcgtcgctgt tgctgtcaca gccgctcggg cagcaaccgt cttggccacc gcacgtcgtg
     5281 atcggtgtgc tcgcgcaagc gacattgcag ctcgcagcgc tgcctgcgat gacgtctttc
     5341 gtgcagacat cgccatccgc gcaggcggcc gggcaggagt cgggcgggtc gcaggtctcg
     5401 tttggatcga gcgtgccgtc gccgcacgtg gaggcgagca cgtccgcgtc cgcgtccgga
     5461 gtctgatttc cgttcgatga agacttacac ccggttgcga tcagcaatcc gccgacgagc
     5521 aagcgtcccc atcgcatacg tcgatgatgc cccgaatcga gggtcatggg ggtgtggtat
     5581 acggaccgcg cccccgatga ttcctcgtta cagtcggccg gagctcgcgc aactctggga
     5641 agacaaaacg cgtttcgagc tgtggctcga tatcgagctc gccgcctgcg aggcgatgga
     5701 gaagcgcggc acggtgcctg cgggaacggc tgcgaaggtg cgagaggccg cgaagggcaa
     5761 gctcgatccc gcgcgcattc tcgaaatcga agcgaccact cgccacgacg tcatcgcgtt
     5821 tctcacgcat gtcgaggagc tcgccggaga gcccgcgcgt tggctgcatc tcggcatgac
     5881 gtcgtcggac gtcctcgatt cctgtctcgc gatccagacg ggcagggcgc tcgagaagat
     5941 cgtgcaaggc gtgatgggcc tcgcgcaggc gctcgccgac cgagcgcgcg aacatgcgaa
     6001 gactccgacg atcggccgct cgcacggcat tcacgccgag ccgatcacgg cgggcctcgt
     6061 attcgcgcgg tggcacgccg aagtcgtgcg cgcatcgagc cgcatcattc gtgcgcggca
     6121 tgcgatcgcg gtcggcaaga tcgccggtgc ggtcggcgtg tacggcaatc tcgatccgga
     6181 gatcgaacgc gaagcgctcg gcgcgctgag cctcgagccg gagacggtgg cgacgcagat
     6241 cgtcgcgcgt gatcgccacg ctgaagtgtt ctgcgcgctc gcgctgctcg gcaccgcgat
     6301 cgagcagatc gcgctcggtg ttcgccactg gcagcgcacc gaagtcggcg aggcggaaga
     6361 gggcttcacg ccgggccaaa agggctcgtc ggcaatgccg cacaagaaga acccgatcct
     6421 cagcgagaac ctcacggggc tcgcgcgcct ccttcgcagc tacgcgggtg cggcgctcga
     6481 ggatgtggcg ctgtggcacg agcgcgatat ctcgcattcc agcgtcgagc gtgtggcgat
     6541 gcccgacgcg acgatcctcg cggacttcat gaccgcgcgt tgcacgaacc tcgtgaagaa
     6601 cctcgtcgtc aatccgcgcc gcatgaaggc gaacctcgat cgcaccggcg agctgttctt
     6661 ctcggaagcg gtcctgctgg cgctcgtcaa gacgggcctg ccgcgccaag aagcgtacgt
     6721 gatggtgcaa cgcaacgcgc tcaaggcgat cagcgaggcg tcgtcgatgg gccacgtcgg
     6781 cgcgctgccg ggacggtttc gcgagctgct cggccaagac gctgacatca agcagcggct
     6841 cgaatccaag gcgctcgacg catgtttcga tctcgagcat cacctgcgcc acgcacccga
     6901 gattctcgag cgcgcgctcg gtcctgcgcc gcgtaacgat ggaggctcga attgacgact
     6961 ctcgacgatc agcttcgcgc ccagctcgac caaacgctcg tcgagacgcc gtttcgatcg
     7021 ctcggcggcc gcacgttgcc gcgctacgac ggcaaggttc gcgactgctt catcgataaa
     7081 gaccgcggcg agcgcgtcat catcgtgacg gatcgcctct cggcgttcga cgcggtggtc
     7141 ggcacgattc cgttcaaagg gcaggtgctg aaccagctcg cgcagttctg gttcgagcag
     7201 acggccgcgc tcgcgcccaa tcacatgctg cgcgtgcccg accccaacgt gatgatcgcg
     7261 cgcgaatgcg atccgctgcc ggtcgagctg gtgatgcgct cgtacctgac gggcgtcacg
     7321 tcgacgtcga tctggaaggc gtacgagcag ggcaagcgca cattctgtgg ccacgcactg
     7381 cccgagggca tgaagaagaa cgagaagctg cccaaagcga tcctcacgcc gtcgacgaag
     7441 gcggccaaag gcgatcacga catctcggtg tcgcgcgacg agctgctcgc gatgggcaag
     7501 atcgagcccg ccgtcttcga ccgcgccgcc gcgatcgcgg agaagctgtt cgcagcgggg
     7561 caggcgcacg ccgcgaagcg cggcctcatc ctcgccgaca cgaagtacga gatgggcctc
     7621 gatcgcgacg gcaacatcat catcatcgac gagatccaca cgcccgattc gtcgcgctac
     7681 tggtacgccg acgactacga agcgcgcctc gccaagggcg aagagccgcg cagcctcgac
     7741 aaggagtacg tgcggcgctg gctcgcaggc gaagcgaagt ggagcggcga tgggccaccg
     7801 cccgtcatgc cgcccgacat tcgtatggag gcggcgcgcc gctatatcgc gagctacgag
     7861 ctcgtcacgg gcaagcagtt catgccggac acgcgcgagc ccatcgcacg gatcgccgca
     7921 gcgctggagg cgaagtgagc ctcgtgcgtg cgctcgcagc aactgctgca gcatggctag
     7981 ccatcgcgcg gatcgccggc gcactcggag tgaagtgacg tgaccgctcc gcagctgcac
     8041 atttggccgg gacgtccggc gctgtctgcg gcgcgcaagg ccgcaacgct cgcacgcatt
     8101 cgcgcggtca gtcccgacgt gacgtccgtc gaagcgcggt ggatgcacat catcacgtgc
     8161 ggtcgcgagc tgacagcaca ggagaccgag cagctcgcgc agatgctgag ctacggaccg
     8221 gcggagccgc cgcagaagtc gaacaggcgg ctcgagaccg tcgagaccgt cagcttcgtg
     8281 atcacgccgc gcgttggcac ggtgtcgcca tggtcgagca aggcgacaga tatcgcgcac
     8341 gtctgcgggc tgacggcggt cacgcgcatc gagcgaggca tcgaatggac gatggtcggc
     8401 gattcgatcg aggtgccggt catcgccgcg ctgctcagcg atcgcatgac cgagagcgtg
     8461 atcatgcgcg agcaggatct cgcgaaggtc gtcgcacccg ccggtgagcc gcgaccgttg
     8521 cagttcgtgc gtctcggcga gcgcggcgcg gacacgctgc gcgaagcgag ccagaagatg
     8581 ggcctcgccc tcgccgaaga cgagatcgag tacctcgtca cgcgctaccg cgagctcggc
     8641 cgcgacccga cggacgtcga gctgatgatg ttcgcgcagg cgaatagcga gcactgtcgc
     8701 cacaagatct tcaacgccga ttggttcatc agcgaggaga agcaggagcg ctctctcttc
     8761 cagtggatca aggagtcgac gaagaacgcg ccgaccggcg tgctctccgc gtacaaggac
     8821 aacgcggcgg tcgtcgaggg caacgtcgcg gaccgcttct tccccgatcc cgacggcgtg
     8881 tatcgcgcgc atcgcgagcc gagccacatc ctcggcaagg tcgagacgca caatcacccg
     8941 acggccatct cgccattccc cggtgcggcg accggctcgg gcggggagat ccgcgatgag
     9001 ggcgcgacgg gccgcggagc caagcccaag gccggcctcg tcgggttcac ggtcagcgac
     9061 ctgcgcattc ccgatgcact cgagccgtgg gaaccgcgcg gcgagcaatg gatgggctcg
     9121 cctcgtcgca ttgcgagcgc gctcgacatc atgatcgacg gcccgctggg cggcgccgca
     9181 ttcaataacg agttcggccg ccccgcgatc ctcggttact tccgcacgtt cgagctcggc
     9241 gaccgcggct atcacaagcc cgtcatgctc gccggtggca tcggcgcgat ccgtccgcac
     9301 cacgtgcaga aggcggcggt ccccgcaggc gcagcgctcg tcgtcctcgg tggtcctgca
     9361 ttcctgatcg gcctcggcgg cggcgctgcc agctcgctcg cgcaaggcgc gtccgccgag
     9421 gatctcgact tcgcgtcggt gcaacgcgac aacgccgagg ttcaacgccg ctgccaagaa
     9481 gtgatcgatc gctgctgggc ggcgggcgag gcgaacccga tcctatcgat ccacgatgtg
     9541 ggggcgggcg gtctctcgaa tgcgatgccc gagctcgtca acgacgccgg cctcggcgcg
     9601 aagctcgacc ttcgcaagat tccgacgggc gagcccgact tgtcgccgct cgagctgtgg
     9661 tgcaacgaat cgcaggagcg ttacgtgctc gcgatcgcgc ccgatcgcat cgaggagttc
     9721 gcggcgctgt gtgctcgcga acgcgcaccg tgggctcagc tcggtgtcgc aacgccgcct
     9781 gtgcccggcg tcgagggcag tcctcagagt gggcgcttga ccgtcgccga cgtaaagggc
     9841 ggcccgccgc ccgtcgatct tccgctcgag ctcgtgctcg gcaaaccccc gcgcatgacg
     9901 cgcaaggcgg agccgctacc ctcgccgagg cggccgctcg acttcgcagg cgcgtcggtg
     9961 gccgaggcgc tcgaccgcgt gctcggcctg ccaaccgtcg ccgacaagtc gttcctcgtc
    10021 acgatcggcg atcgcacggt cggtggcctc gtcacacgcg atccgatgat cggtcgcttt
    10081 caggtgcccg tcgcggattg cgcgttgacc accgccggct tcgatacgta cgcgggcgaa
    10141 gtgatgtcga ttggcgagcg tccacccgtc gcgctcctcg atgcggctgc ggcgtcgcgc
    10201 ctcgcgattg gcgaagcgat cacgaatctc gccgctgcgc cgattggaat tgcgggtcgt
    10261 gcaccgggcg agccgctcgg accgggcgac ctttcgtcga tcaagttgtc gtgcaactgg
    10321 atggcggcgg caggtcatcc cggcgaggac gcgcgcctgt acgaagccgt gcgtgctgcg
    10381 ggagagacgg cggtcgcgct cggtatctcg attcccgtcg gcaaagattc gatgtcgatg
    10441 cgcacgcagt gggcaggcca gggtgatgag cgtcgcgtga tcgcatcgcc ggtcacgctc
    10501 gtcgccacgg cgttcggccc ggtcacggat gttcgctcca acgtcacgcc cgagcttcgc
    10561 ggcacgggga ctctcctgtt cgtcgatctc ggcaacggca agaaccgcct cggcggcagc
    10621 tgcctcgcgc aggtctacgg ccagctcggc gatgcgccac cggatctcga cgagccggcg
    10681 cgcctgcgga gcttcttcac cgtgatgcag tcgctcgtcg ctgcgggcac gctgctcgcg
    10741 tatcacgacc gctccgacgg cggcctcatc gtcgcggcgc tcgagatggc atgggccggc
    10801 ggcctcggcc tcgagctcga cgtcacgaac gtcgacgccg atccgttccg cgcgctgttc
    10861 tccgaggagc tcggcgcgct gctcgaagtc gcatccgcgg acgtgcccgc cgtgcgcgcg
    10921 gcattcgaag ctcatggcat cgccgtgcag ccgatcggtc gcggcgtcga aggcaatcgc
    10981 gtcgtcgtcc tccacggtga ggcgacgaaa gttatcgacg agtctcgctt cacgctgcgc
    11041 gcgcgctggt cgcacgtcac tcgcgagatc gcgcggcgcc gcgacgacgc cgactgcgca
    11101 tccgaagagc acgaggcgcg cgtcgaacgc accaacatcg gcctcaccgc cgaagtgcgc
    11161 ttcgatccaa accaggacat cgcagcgccg ttcatcgcga ccggcgtgcg gcctcgcgtc
    11221 gcgatcctgc gcgagcaagg cgtcaacggc cagatcgaga tggccgccgc gttcagccgc
    11281 gcgggcttcg acgcgatcga cgtccacatg accgacctca tcgaaggtcg cgtcgacctc
    11341 tccgattgcc gcggcgccgt ggcatgcggc ggcttctcgt tcggcgatgt gctcggtgca
    11401 ggccgcggtt gggcagcaac gtttcgctac aacgcgcgcg cgcagtcagc gattcgcagg
    11461 ttcgtcgacc gcgcagacac gttcctgctc ggcgtctgca acggctgcca gatggtcgcc
    11521 gacctcgcgc ccgacctcgt cgagagcacg cgcgatgtgt ggcctcgctt cgtcaagaac
    11581 cgtagcgagc ggttcgaagc gcgcgtcgtg atgctgcgca tcga
//
LOCUS       DATLBZ010000584        11150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7135213, whole genome shotgun sequence.
ACCESSION   DATLBZ010000584 DATLBZ010000000
VERSION     DATLBZ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7135213"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VMZ53_28845"
     CDS             complement(<1..327)
                     /locus_tag="VMZ53_28845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32557.1"
                     /translation="MPALPEATDVAQAMQGTGDVAQAMHGTGDVARAIGGTGDVARAM
                     HGPGDVPPAFAGCDLASAVVRRGPGRSDVQRVRGAGPLRVLCPRSAGNAAWLVTSSLG
                     GGLVDGD"
     gene            352..1128
                     /locus_tag="VMZ53_28850"
     CDS             352..1128
                     /locus_tag="VMZ53_28850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HUS32558.1"
                     /translation="MPVHLREVVGYLNQTLEIERFKDYAPNGLQVEGALEVERVVTGV
                     SASAELISRAIELHADLVVVHHGLVWGHGLTSVTGPLARRLKLLLGNDVSLAAYHLPL
                     DKHARLGNNTGLADALALGPQREAFGDVKGVALGLAGSWSTPLSRNDAVARVGGGVCK
                     GQSPRFAFPYGPEQVRKVGVCSGAASDLLEAAAAAGCDMFVTGELAERAGDLARELQI
                     TLVAAGHVATEVFGPMRLAEEIKMRFPGVEAQFVHVANPL"
     gene            1300..2511
                     /locus_tag="VMZ53_28855"
     CDS             1300..2511
                     /locus_tag="VMZ53_28855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HUS32559.1"
                     /translation="MLSVVPIATSLLVAGSGCLGDTDDDLVVSETSQAATVSTYVSGS
                     CSTAVVIGLSKQIADQISCDSPTLLKRFTATGNLQVSSNAVLPYLHSTAKTDLESVAA
                     TRVVQVNSAFRALPQQYLLYRWFQQGRCGITAAATPGRSNHQSGRALDVANYSSLISA
                     MSNRGWAHDVPGDAVHFDHLSSPDIRGKDVLAFQKLWNRNHPTDKIGEDGAYGPQTEA
                     RLKLAPATGFAIGPSCVTSSTALVADVISVDGPDRVDTQQRAHYSITIQNNSEREWPG
                     SAVVRVMGTGSQLHDASWLSATNIASLGAAIPAHEAGELAFDITTPLVTEETPIFEEL
                     EISDGATHLGTVSIALTVIPGMGEEPTSSDSDDQYDQEVTGGCSAGGNGSQTGVVLSL
                     ALLALIRRRRR"
     gene            2608..3327
                     /locus_tag="VMZ53_28860"
     CDS             2608..3327
                     /locus_tag="VMZ53_28860"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HUS32560.1"
                     /translation="MTRLATLLSLTVLAGIANGCADNPGVGEDPEYGSGEGVDYDEEN
                     PNAPLDDGKSDAPRYQIPTNLPELVSPEIIVSLDGLTVHLFDRATGFSEVYPAGVGQK
                     NSRGISITPTGHFKSGADGNDGWWYAPRRTVPDYFGGFPFLRTTARNSDGANTYALHG
                     PITATLQRGYVSHGCVRMRGNDIVRLFWMVKKHPNTPITIQKEWERDAAGKKVDVGTN
                     PTLWPVGAQITFGASVGPRPF"
     gene            3338..4318
                     /locus_tag="VMZ53_28865"
     CDS             3338..4318
                     /locus_tag="VMZ53_28865"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HUS32561.1"
                     /translation="MRALLTLSLVACSAPATPPAKPAKPVDSSPRMAWGLVLHGGAGT
                     MSRDKLTPEREAIMRADLERALREAHGVLSRGGTSTDAVTAAIVILEDSPYFNAGKGA
                     VFTHDGINELDASIMDGKTRTAGAVAGVRTVKNPILLARAVMEKSRHVMMVGSGAEEF
                     AATVGIERVDPSYFRTEERWQELQDKLKDDKFGTVGVVALDKTGGLAAGTSTGGLTNK
                     RYGRVGDSPIIGAGTYADERCGVSATGHGELFIRYTVAHDICARMEYTGVPLAQAAHD
                     VVEDELVKAEGDGGIIAMDAAGHWALTFNTPGMYRGYITEEGIPHVDIYR"
     gene            4333..7707
                     /locus_tag="VMZ53_28870"
     CDS             4333..7707
                     /locus_tag="VMZ53_28870"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HUS32562.1"
                     /translation="MGIRGRCIALWLFIMCAFVPATASAQSATTGAIYGVITDAGTGD
                     PLPGVTVVVTSTGSASQTALTDEKGSYKVNDLAPGEYLVTFYYLDAALERPNVPVGVQ
                     KVTPLYQKIDTANAMKGEVVQVKGTTPTIDPTSTAQGITIDKNYLQNSPVPGRTFESA
                     LGAAAGSQGDIGGVAFSGSTSLENQYYVDGVNTTGLSYGTVGSPVINDFIEEIEIITG
                     GYNAEYGRATGGIVNVVTKSGSNEFKGSIFGYMQPGFLTAEAERTPANATSIDIKGDV
                     AYQTDVGFELGGPIIKDKVWFFIGFAPQLTNIAYTRDVKRQTDCHMVQDDGKLSVCNP
                     MYADSEPDIDPATGFYETDIVDTEVRHSTSQVYNTLAKINYAATPEQQGQLAVQAQPG
                     ANRRPNLYGPGGTGTTAKFLGVDVSAKWTAKLNNNKTELEGFIGGHRETYKNQSVDPA
                     LQGLPLQILVDGNLGVWGPGFGESLDVNSGCFDDPTGKSDAYPLITNCPMTARAYYIG
                     GPGSLYDDTDQRIAARVGVTQRVKAGGSHEIKGGVDFEDNRTEHTRSLSGGAYVINYL
                     DQQADTVRWVQIGTPETTDPRFDNMCHTPAPPTFPGAQTDFTCDYLSGVPGDDGTMIE
                     GNTFNWATYLRDSWQIRPNLTLNAGLRYEEQRLRLAKFLRNEIDVASGDRLGKNALVL
                     RGELAPRIGLLYDWTKEGRSKAYAHWGRFYESIPMEINDYNFGMPVQYVQTYTPSLCT
                     TMDDPRIGGPDGAACLTTDKKPDLRQELFGVYGSLTAPGVKGQYMDEVVGGIEFEIID
                     DLKLGVSFQDRRFGRVIEDVSLDNANTYVVSNPGEWSAEEERKFQQRVDAETDETKKQ
                     RLMRQLEMFRGIRIFDKPRRNYDALQFTMTRRFSKHLYTQGSYTYSRTFGNYPGLINY
                     DDNVVLPNNSTQYDLIELLANRIGPLPQDRPHYIKLDAYYQFDLKKYGDLTTGIRARA
                     LSGTPKSALAPHYLYGDDQSFLVPRGAMGRSSFEHELDIHVGYGRKMHAGKREIHGEL
                     FLDIYNVFDNQGQAAIDQSYATIVRRGTGMAGAGGTIQSANPVSGGTYEDLRWVKVID
                     RNGVESSTPLGRNPNYGNTTARYSPLYARFGARVTF"
     gene            7762..8103
                     /locus_tag="VMZ53_28875"
     CDS             7762..8103
                     /locus_tag="VMZ53_28875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32563.1"
                     /translation="MLKKIVVALVVVFGSACIDDGVNGTPGSLPTDETPEEPGIDTPA
                     EMPQTALTERSRQQNPGDPAVCELLPQDDSACAHACDEDVLLTYIPEGTCASFACPLT
                     DGTTFVTGGCN"
     gene            complement(8063..8665)
                     /locus_tag="VMZ53_28880"
     CDS             complement(8063..8665)
                     /locus_tag="VMZ53_28880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HUS32564.1"
                     /translation="MRRLIVLSFALTSALVASVRTAEANSCNLYYEQRFGMFREGCSV
                     TTFMLQGQMPHLPAVKRNGQELTPTLVQDEITLKVRFDHYPSADSCELLPPVYENRTF
                     DRYVMTWDLQGGDEIVVDNSPIVVPGPGDCGGVPEPAFYCTDPIQSCNDPVDPDPDTD
                     NLGDDTAGCSAGSGSASWLSLLALIGLVSYSRRSRTSSRR"
     gene            complement(8827..9543)
                     /locus_tag="VMZ53_28885"
     CDS             complement(8827..9543)
                     /locus_tag="VMZ53_28885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32565.1"
                     /translation="MRSVLVAALPFLLLACGDDGGGGGGNPDAASNVPAMITVSGKAT
                     KREGTNSSAAAGVVVSAYQSTDPNTPVATATTDAAGMYSMVITTNGKALDGFLKGTLT
                     GFLDTYLYAPAPLDADFSSASINMINSNTLGLLSGTLCASAQDNAKGVIALIIADSSR
                     VEVAGATASSAPAAAKVCYNQGGFPNKNATMTDTDGIAYLLNVTAGKVTVNAAGAGAP
                     YHSHDVEARAGTFTTTVIQP"
     gene            9604..10815
                     /locus_tag="VMZ53_28890"
     CDS             9604..10815
                     /locus_tag="VMZ53_28890"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HUS32566.1"
                     /translation="MPSGVRDFLPRAAARRRSLAEKVLEVFEAWGYARLITPMFECAD
                     VLERGLGAGARAAAIRFVEPGTGEVVALRPDITPQIARVVATRMAEIPGPIRLCYEGA
                     VTRLAGEHGQREILQAGIELVDAPEPDGDAEVLAVAASALARLDLPETRLDVGHVAPA
                     AFVLSTAPDPGARLMLVAALGRKDRAGLRVAARVLPEGIAPLAEALATLWGPASATLD
                     RAEALPWPAPVKQSLAQLRAVLAAFGALAEPPVPSLTIDLGDLRGFDYYTGVRFAAYA
                     GGAPDAVLRGGRYDELLAKYGRAAQATGFAIDLEAVAQAQRSIGVAAPAAGFGVAVDE
                     PELARAIRKRGIRVVTQQRAPASWGAWLRGAGLDAAVVGDRLIRHDDSARDLNGARED
                     IDRLIALLKST"
     gene            10816..>11150
                     /locus_tag="VMZ53_28895"
     CDS             10816..>11150
                     /locus_tag="VMZ53_28895"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HUS32567.1"
                     /translation="MSVVIVVGAQWGDEGKGKVVDLFTERAHTVVRYGGGANAGHTLV
                     IEGKKLVTHLVPSGVCHPGKACVLGDGMVIDPHTLLEEIAECQERGLLMRNELLIGLG
                     AHVILPYHKL"
BASE COUNT         2012 a         3674 c         3638 g         1826 t
ORIGIN      
        1 atcgccatcg acgagtccgc cgccgagact gctcgtcacg agccacgccg cattgccggc
       61 tgagcgtggg cagagcactc gcaacggccc cgcacctcgc acgcgctgaa catcgctgcg
      121 gccaggaccg cgccgcacca ccgcggaagc gagatcgcaa cccgcaaacg ccggcggcac
      181 atcgcccggg ccgtgcatgg ctcgcgcgac atcgccggtg ccgccgatcg ctcgtgcgac
      241 atcaccggtg ccgtgcatcg cctgcgcgac atcaccggtg ccttgcatcg cctgcgcgac
      301 atcggtggct tcgggtaacg cgggcaccgc cggtactgta cgctcgccgc catgcctgtg
      361 catctgcgcg aggtcgtcgg gtacctcaat cagacgctcg agatcgagcg cttcaaggac
      421 tacgcgccta atggcttgca ggtcgagggc gcgctggaag tcgagcgcgt cgtcaccggt
      481 gtgtccgcgt cggcggagct catctcgcgc gcgatcgagc tgcacgcgga tctcgtcgtc
      541 gtgcatcacg ggctcgtgtg gggacatggc ctcacgtccg tgacgggccc actcgcgcgg
      601 cgcctgaagc tgctgctcgg caacgacgtg tcgctcgcgg cgtatcactt gccactcgac
      661 aagcacgcgc gcctcggcaa caacaccgga ctcgccgatg cgctggcact cggcccgcag
      721 cgcgaagcgt ttggcgacgt gaaaggcgtc gcgctcgggc ttgcgggcag ctggtcgacc
      781 ccgctttcgc gcaatgatgc ggtcgcgcgc gtcggcggcg gcgtgtgcaa gggacagtcg
      841 ccgcggtttg cgtttccgta tggaccggag caagtgcgca aggtcggcgt gtgctcgggc
      901 gcggcgtcgg atctgctcga agcggcggca gccgcgggtt gcgacatgtt cgtcaccggc
      961 gagctcgccg aacgagccgg cgatctagcg cgcgagctgc agatcacgct cgtcgctgcg
     1021 gggcacgtcg cgaccgaggt gttcggaccg atgcgcctcg ccgaggagat caagatgcgg
     1081 tttcccggcg tcgaagctca gttcgttcac gtcgcgaatc cgctctagcg ctgtcgatca
     1141 tgaagctgtg cgcgtagttg cgcgtggtgt gcagcgtcgt gatcaacgtg tttcggcgtg
     1201 gtcgttgctg aaggcaagcg cgccatgccg tccctcacca agcttgatcg agttatcgcg
     1261 accttcacgc aatcacagcc tgggagtgcg ctcttcaggt tgctgagtgt cgtccccatt
     1321 gcaacctcgc tgctcgtcgc tggcagcggc tgtcttggcg acaccgacga cgatctcgtt
     1381 gtcagcgaga cctcgcaagc ggccacggtg tcgacgtacg tgagcggcag ctgctcgacc
     1441 gccgtcgtga tcggtctctc taagcagatc gccgatcaga tcagctgcga tagcccgaca
     1501 ctgctgaagc gcttcaccgc gacgggcaat ctccaagtct cgagcaacgc cgtgctgccg
     1561 tatctgcatt cgaccgcgaa gacagatctc gaatcggtgg ccgcgacgcg cgtcgttcaa
     1621 gtcaactcgg cgtttcgcgc gctgccgcag cagtacctcc tgtaccgttg gttccagcaa
     1681 ggccgttgcg gcatcaccgc tgccgcgacg ccgggccgct cgaatcatca gagcgggcgc
     1741 gctctcgatg tcgcgaacta ctcgagcctc atctcggcga tgagcaaccg cggctgggcg
     1801 cacgacgtgc ccggcgatgc ggttcacttc gatcatctct cgtcgcccga tattcgtggc
     1861 aaggatgtcc tcgcgttcca gaagctctgg aaccgcaatc atccgaccga caagatcggc
     1921 gaggacggcg cctacggccc gcagaccgaa gcgcggctca agctcgcacc ggcgaccggc
     1981 ttcgcgatcg gcccgtcgtg cgtgacttca tcgacggcgc tcgtcgctga cgtcatctcg
     2041 gtcgacggcc ccgaccgtgt cgatacgcag cagcgcgcgc actactcgat cacgatccag
     2101 aacaacagcg agcgtgagtg gccgggttcc gccgtcgtac gcgtcatggg tacgggctcg
     2161 cagctgcacg atgcgagctg gctctcggcg acgaatatcg cgtccctcgg tgcggcgatt
     2221 ccggcgcatg aagcgggcga gctcgcgttc gacatcacga cgcctctcgt gaccgaagag
     2281 acgccgatct tcgaggagct cgagatttcg gacggcgcta cgcacctcgg caccgtgagc
     2341 atcgcgctga cggtcattcc gggcatgggc gaagagccga cgagcagcga ctcggatgat
     2401 cagtacgacc aagaggtcac gggcggctgt tcggccggcg gcaacggctc gcagacgggc
     2461 gttgtgctga gccttgcact actcgcgctg atccgtcgtc gacggcgcta gtctcccggc
     2521 gagtcgagga gccaggctcg cgggcgagaa gcccgcgttt tcattagagc ggctcgcggc
     2581 acagagcgtg ctcatgcggc cgtcgacatg acccgcctgg ctactctgct ctcgctgaca
     2641 gttctcgccg gaatcgcaaa cggttgcgcc gacaacccgg gcgttggaga ggatcccgag
     2701 tacggctcgg gtgagggcgt cgattacgac gaggagaacc cgaacgcgcc gctcgacgac
     2761 ggcaagtccg atgcgccccg ctaccagatt ccaacgaatc tacccgagct cgtctcgccc
     2821 gaaatcatcg tctcgctcga cggcctgacg gtccacctct tcgaccgcgc gaccggcttc
     2881 tccgaggtct accccgcggg cgtcggccag aagaactcac gcggcatcag catcacgccg
     2941 accggccact tcaagagcgg cgccgacggc aacgatggct ggtggtacgc gccacgccgc
     3001 acggtccccg actacttcgg cggcttcccg ttcctccgca ccacggcacg caacagcgac
     3061 ggcgcgaaca cgtacgcgct gcacggtccg atcacggcga cgctccagcg cggctacgtc
     3121 tcgcacggct gcgtgcgcat gcgcggcaac gacatcgttc gcctgttctg gatggtcaag
     3181 aagcacccga acacgccgat cacgatccag aaggaatggg agcgcgacgc ggctggcaag
     3241 aaggtcgacg tcggcacgaa cccgacactg tggcccgtcg gcgcgcagat caccttcggc
     3301 gcctcggtcg gtccgcgtcc gttctagact gcgcgccatg cgcgcgcttc tcactctctc
     3361 tctcgtcgct tgctcggcgc cggcaactcc gccggcgaag cccgcgaaac ccgtcgactc
     3421 gtctccgcga atggcgtggg gcctcgtcct ccacggcggc gccggcacga tgtctcgcga
     3481 caagctgacg cccgagcgcg aagcgatcat gcgcgcggac ctcgagcgag cgctgcgcga
     3541 ggcgcacggc gttctgtcgc gcggcggcac gagcaccgat gcagtaaccg cggcgatcgt
     3601 gatcctcgag gactcgccat acttcaacgc gggcaagggc gccgtcttca cgcacgacgg
     3661 catcaacgag ctcgacgctt cgatcatgga tggcaagacg cgaaccgccg gcgccgtggc
     3721 gggcgtgcgt acggtcaaga atccgatcct gctggcccgc gcggtcatgg agaagagccg
     3781 gcacgtcatg atggtcggca gcggcgccga ggaattcgcg gccaccgtcg gcatcgagcg
     3841 cgtcgacccg tcgtacttcc gcacggaaga gcggtggcag gagctgcagg acaagctgaa
     3901 ggacgacaaa ttcggcaccg tcggcgtggt cgcgctcgac aagacaggcg gcctcgccgc
     3961 aggcacctcg accggaggtc tgaccaacaa gcgctacggc cgcgttggcg actcgcccat
     4021 catcggcgcg ggcacgtacg ccgacgaacg ctgcggtgtg tcagcgaccg ggcacggcga
     4081 gctgttcatc cgctacacgg tcgcccacga tatctgcgcc cgcatggagt acacgggcgt
     4141 cccgctcgcc caggcggcgc acgacgtcgt cgaggacgag ctggtcaagg ccgagggcga
     4201 cggcgggatc atcgcgatgg atgcggcggg tcattgggcg ctgaccttca acacccccgg
     4261 catgtaccgg ggctacatca ccgaagaagg aatcccccac gtcgacatct atcggtgata
     4321 cgaaacaggc ccatggggat tcgcggtcgt tgtatcgcgt tgtggctgtt catcatgtgc
     4381 gcgttcgtgc cggcaacggc atcagcgcaa tccgcgacga cgggcgcgat ctacggcgtc
     4441 atcaccgacg cgggcacggg cgatccgttg ccgggcgtca ccgtcgtcgt gacgtcgacc
     4501 ggcagcgcga gccaaacggc gctgaccgac gagaagggct cgtacaaggt caacgacctc
     4561 gcgcccggtg aatacctcgt cacgttctac tacctcgatg ctgcgctcga gcgaccgaac
     4621 gttcccgtcg gcgtccagaa ggtcacgccg ctctatcaga agatcgacac cgcaaacgcg
     4681 atgaagggcg aggtcgtgca agtcaaaggc acgacgccga cgatcgatcc gacgtcgacc
     4741 gcgcagggca tcacgatcga caagaactac ctgcagaaca gtccggtgcc gggacgcacg
     4801 ttcgaatcgg cgctcggtgc agccgcgggc tcgcaaggcg atatcggcgg cgtggcgttc
     4861 tccggatcga cgtcgctcga gaaccagtac tacgtcgacg gcgtcaacac gacgggcctc
     4921 tcgtacggca cggtcggttc gccggtgatc aacgacttca tcgaggagat cgagatcatc
     4981 acgggcggct acaacgccga gtacggccgc gcgacgggtg gcatcgtcaa cgtcgtcacg
     5041 aagagcggct cgaatgaatt caaaggctcg atcttcggct acatgcagcc gggcttcttg
     5101 acggcggagg ccgagcgcac gcccgcgaac gcgacgtcga tcgacatcaa gggcgacgtc
     5161 gcctatcaaa cagatgtcgg cttcgagctc ggtggcccga tcatcaagga caaagtctgg
     5221 ttcttcatcg gcttcgcgcc gcagctcacg aacatcgcgt acacgcgcga cgtcaagcgg
     5281 cagaccgact gccacatggt gcaggacgac ggcaagctga gcgtctgcaa cccgatgtac
     5341 gccgactccg agccggacat cgatccggca acgggcttct acgagaccga catcgtcgac
     5401 accgaagtcc gccactcgac gtcgcaggtc tacaacacgc tcgcgaagat caattacgcg
     5461 gcgacacccg agcagcaagg tcagctcgct gtgcaagcgc agcccggcgc gaaccggcgc
     5521 ccgaacctct acggtcccgg cggtaccggc acgaccgcga agttcttggg cgtcgacgtc
     5581 tcggcgaagt ggaccgcgaa gctcaacaac aacaagaccg agctcgaagg cttcatcggc
     5641 gggcatcgcg agacgtacaa gaaccaatcg gtcgatccgg cgctgcaggg gctgccgctg
     5701 cagatcctcg tcgacggcaa cctcggcgtg tggggcccag gcttcggcga atcgctcgac
     5761 gtcaactcag gttgcttcga cgatccaacc ggcaagtcgg acgcgtaccc gctcatcacg
     5821 aactgcccga tgaccgcgcg cgcgtactac atcggcggtc ccggctcgct gtacgacgat
     5881 accgatcagc ggatcgcggc gcgcgtcggt gtcacgcagc gcgtgaaggc gggcggcagt
     5941 cacgagatca aaggcggcgt cgacttcgag gacaaccgca cggagcacac ccgctcgctc
     6001 tccggcggcg cgtacgtcat caactatctc gatcagcaag ccgacaccgt gcggtgggtg
     6061 caaatcggca caccggagac gacagatccg cggttcgaca acatgtgcca cacgccagcg
     6121 ccgccgacgt tcccgggcgc gcagacggac ttcacgtgcg actacctcag tggcgttccc
     6181 ggcgacgacg gcacgatgat cgagggcaac acgttcaact gggcgacgta cctgcgcgac
     6241 tcctggcaga tccgcccgaa cctgacgctc aacgcgggcc tgcgctacga agagcagcgg
     6301 ctgcgcctcg cgaagtttct gcgcaacgag atcgatgtcg cgagtggcga tcggctcggc
     6361 aagaacgcgc tcgtgctgcg cggcgagctc gcacctcgca tcggcctgct ctacgactgg
     6421 acgaaggaag gtcgctcgaa ggcgtacgca cattggggtc gcttctacga atcgattccg
     6481 atggagatca acgactacaa cttcggcatg ccggtgcagt acgtgcagac gtacacgccg
     6541 tcgctgtgca cgacgatgga cgacccgcgc attggtggtc ccgacggcgc tgcgtgtctg
     6601 accaccgaca agaagccgga cctgcgccaa gagttgttcg gtgtctacgg ctcgctgacc
     6661 gcgccgggcg tgaagggcca gtacatggac gaggtcgtcg gcggcatcga gttcgagatc
     6721 atcgatgacc tcaagctcgg cgtgtccttc caagatcgtc ggttcgggcg cgtgatcgag
     6781 gacgtctcgc tcgataacgc gaacacgtac gtcgtgagca atccgggcga gtggtccgcc
     6841 gaggaggagc gcaagttcca gcagcgcgtc gatgccgaga ccgacgagac aaagaagcag
     6901 cgcctcatgc gccagctcga gatgttccgc ggcatccgca tcttcgacaa accgcgccgg
     6961 aactacgacg cgctgcagtt cacgatgacg cgccgattct cgaagcacct ctacacgcaa
     7021 ggctcgtaca cgtactcgcg cacgttcggc aactaccccg gtctcatcaa ctacgacgac
     7081 aacgtcgtgc tgccgaacaa ctcgacgcaa tacgacctga tcgagctgct cgcgaatcgc
     7141 atcggcccgc tgccgcaaga tcgcccgcac tacataaagc tcgacgcgta ctaccaattc
     7201 gacctcaaga agtatggcga cctgacgaca ggcattcgcg cacgcgcact ctccggcacg
     7261 ccgaagagcg cgctcgcacc gcactatctc tacggcgacg accaatcgtt cctcgtaccg
     7321 cgcggtgcga tgggccgctc gagcttcgag cacgagctcg acattcacgt cggctacggc
     7381 cgcaagatgc acgcgggcaa gcgcgagatc cacggcgagc tgttcctcga catctacaac
     7441 gtgttcgaca accaaggcca agccgcgatc gaccagagct acgcaacgat cgttcgtcgc
     7501 ggcacgggca tggccggagc gggcggcacg atccaatcgg cgaacccggt gtcgggcggc
     7561 acgtacgagg acctcaggtg ggtcaaggtc atcgatcgca acggtgtcga atcatcgacg
     7621 ccgctcggtc gcaatccgaa ctacggcaac acgaccgcgc gttactcgcc gctctacgcc
     7681 cggttcggcg cgcgcgtaac gttctagccc gctccgcgaa attcggagat cggcggcacg
     7741 cgtgatgctc cgagtatcag catgctgaag aagatcgtcg tcgcgttggt cgtcgtcttc
     7801 gggtcagcct gtatcgacga cggcgtgaac ggtacgccag gctcgctgcc gacggacgag
     7861 acgcccgagg aaccgggaat cgacacgccc gcggagatgc cacagacagc gctgacagaa
     7921 cgttcgcgtc agcagaatcc gggcgatccg gcggtctgcg agctgctgcc gcaagatgac
     7981 agtgcgtgcg cgcacgcgtg tgacgaggat gtgctgctca cgtacatccc cgaaggaaca
     8041 tgcgcgtcgt tcgcgtgccc acttaccgac gggacgacgt tcgtgaccgg cggctgtaac
     8101 tgacaaggcc gatcagcgcg agcaacgaga gccaactcgc agagcccgag cctgcagagc
     8161 agccggccgt gtcgtcgccg aggttgtcgg tatccggatc tggatcgacg gggtcgttgc
     8221 acgactggat cggatcggtg cagtagaagg ccggctcggg aacgccgccg caatcaccgg
     8281 gacccgggac gacgatcgga gagttgtcga ccacgatctc atcgccgcct tgcagatccc
     8341 acgtcatcac gtagcgatcg aacgtccgat tttcgtacac aggcggcaga agctcgcagc
     8401 tatcggcgga cgggtagtgg tcgaaccgaa ccttcagcgt gatctcgtct tgcacgaggg
     8461 tcggcgtcag ctcctgaccg ttgcgcttca ccgcaggcag gtgcggcatt tggccttgga
     8521 gcatgaacgt ggtcaccgag cagccttcgc ggaacatgcc gaaccgctgc tcgtaataca
     8581 ggttgcagct gttggcctcc gcggtgcgaa ccgaggcgac gagggcgctg gtgagcgcga
     8641 agctaaggac gatcaggcgg cgcatgcctt gccactcagc atgctgcgcg ccacatcctc
     8701 gggttgcatt ctcgcggcgt tagcgcgcgc gtgacgccac cacgcctgtg gatcagggcg
     8761 acgatgtcgc gaccggcgac gccatgacag gcacttacga agtgacgtgc gaagtcgcgc
     8821 cgttcgttac ggctggatga cagtcgtcgt gaacgtaccc gcgcgagctt cgacgtcgtg
     8881 cgagtgatac ggcgcgccag cacccgctgc gttgacggtg accttgccag ccgtgacgtt
     8941 gagcagatac gcgatgccat ccgtgtccgt catcgtcgcg ttcttgttcg ggaagccacc
     9001 ctggttgtaa caaaccttcg cggccgccgg agcgctcgac gccgttgcgc cggcaacttc
     9061 gacgcgtgac gagtccgcga tgatgagcgc gatgacgccc ttcgcattgt cctgcgccga
     9121 cgcgcacagc gtcccggaga gaaggccgag cgtgttcgag ttgatcatgt tgatcgacgc
     9181 gctcgagaag tcggcatcga gcggcgccgg tgcgtagaga tacgtatcga gaaagccggt
     9241 cagcgtgccc ttgaggaagc cgtcgagcgc cttgccgttc gtcgtgatca ccatcgagta
     9301 catgccggcg gcatccgtcg tcgcagtcgc gaccggcgtg ttcggatcgg tcgattgata
     9361 cgccgagacc accacgccgg cggcggcgct cgagtttgtc ccttcgcgct tcgtcgcttt
     9421 gcccgagacc gtgatcatcg cgggcacgtt ggacgcggcg tcggggttgc caccgcctcc
     9481 tcctccatca tcaccgcagg ccaagagaag gaagggaagc gcagctacga ggacggatcg
     9541 catgcctttt tgtacacgag agtcgggcta gagtagggcg ctttgtcgac cccgccgatc
     9601 cgattgccat ctggcgtacg cgattttctg ccgcgtgccg cagcgcgccg acgttcgctc
     9661 gccgagaagg tgctcgaagt attcgaagcg tggggctatg cgcgattgat cacaccgatg
     9721 ttcgagtgtg ccgacgtact cgaacgtggt cttggtgcgg gcgcgcgcgc agccgcgatt
     9781 cgcttcgtcg agccgggcac cggagaagtg gtcgcattgc gcccggacat cacgccgcag
     9841 atcgcgcgcg tcgttgcgac acgcatggcg gagattccag ggccgatccg gctttgctac
     9901 gaaggcgcgg tcacgcgact cgccggcgag cacggccagc gcgagatcct gcaagcaggg
     9961 atcgagctcg tcgatgcgcc cgagcccgac ggagatgccg aagttctcgc cgtggcggcg
    10021 tccgcgctcg cacggttgga tcttccggag acgcgcctcg atgtcggtca cgtcgcgccc
    10081 gcggcattcg tgttgagcac ggcgccggat cccggtgcgc ggctcatgct cgtggcagcg
    10141 ctcggccgaa aagatcgcgc gggtctgcgc gtcgcggcgc gcgtgttgcc cgaaggcatc
    10201 gcgccactgg ccgaggcact cgcgacgcta tggggacctg cgagcgccac gctcgatcgc
    10261 gccgaagcgt tgccgtggcc agcgccggtg aagcaatcac tcgcgcagct gcgcgccgtg
    10321 ctcgctgcat ttggtgcgct cgccgagccg ccggttccga gcctgacgat cgatctcggc
    10381 gatctgcgcg gcttcgacta ctacaccggc gttaggtttg cggcgtatgc gggcggcgct
    10441 cccgatgcgg tgctgcgcgg cggtcggtac gacgagctgc tcgcgaagta tggccgcgcg
    10501 gcgcaggcga cgggattcgc gatcgatctc gaagcggttg cacaagcgca gcgctcgatc
    10561 ggtgtcgctg ctcccgcagc cggctttggc gtcgccgtcg acgagccgga gctcgcacgc
    10621 gcgatccgca agcgaggaat tcgtgtcgtc acacagcagc gtgctcccgc gagttggggc
    10681 gcatggttgc gcggcgcagg gctcgacgcg gcggtcgtcg gggatcgact gattcgccac
    10741 gatgattctg cgcgcgatct gaatggcgca cgcgaggaca tcgaccggct gatcgcgcta
    10801 ttaaagtcga cgtagatgtc ggtcgtcatc gtcgtcggcg cccaatgggg cgatgaaggt
    10861 aagggcaagg tcgtcgacct ctttaccgag cgcgctcaca ccgtcgttcg ctacggaggc
    10921 ggcgcgaacg ccgggcacac gctggtgatc gagggcaaga agctcgtcac gcacctcgtg
    10981 ccgtcgggcg tctgtcatcc cggtaaggcg tgcgtgctcg gcgatggcat ggtcatcgat
    11041 ccgcacacgc tgctcgaaga gatcgcggag tgtcaggagc gcggcctgct catgcgcaac
    11101 gagctcctca tcggtctcgg cgcgcacgtg atcctgccgt accacaagct
//
LOCUS       DATLBZ010000585        11618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8634198, whole genome shotgun sequence.
ACCESSION   DATLBZ010000585 DATLBZ010000000
VERSION     DATLBZ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8634198"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VMZ53_28900"
     CDS             <1..1284
                     /locus_tag="VMZ53_28900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HUS32568.1"
                     /translation="RAGADTALARIARAVEDAQGSKAAIARLADRVSAVFVPIVLTIA
                     TLTFVGWLATGHTATQSLATMIAVLVIACPCALGLATPAAVAVGTARGAELGLLFRGG
                     DALERAASIDVVCLDKTGTLTTGHPRLVSTLDDDVLRLAASAEQHSEHPIARAIVDAA
                     KTKGLALARAENVTVEAGGGLTSVVEGRAIKIGSRAFVASDLARGTSGGSAIATSAGS
                     AIATSGVAAIATGDATSFIVIDGDVRGAIEIADLPRDDAATTIAALRGLGVEPVMITG
                     DKREAADRVAAEVGITRVHAEIKPTGKAALVEAEKARGKRVAMVGDGINDAPALAAAD
                     LGIALATGTDIAAAAADVTLLRGGIGALPTALTLARATMRTIRRNLAAAFVYNAICIP
                     IAAAGLLSPVLASAAMSLSSVSVLASSLRLRRFRA"
     gene            1346..2326
                     /locus_tag="VMZ53_28905"
     CDS             1346..2326
                     /locus_tag="VMZ53_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS32569.1"
                     /translation="MGDAVLEIRDLVTEFRTEQGVVRAVDGVSFSIPARGTLGLVGES
                     GSGKSVTALSIMRLVASPPGRIASGDIRYAGKDLLALPAEEMRAIRGNRIGMIFQEPM
                     TSLNPVYTVGEQVGEAVRLHQKKSKAEARAISIDMFKLVGIPSPAERIDAYPHQLSGG
                     MRQRVMIAMALACKPDLLIADEPTTALDVTIQAQILDLLKKLQSEMGMSILLITHDLG
                     VVAETCDEVVVMYAGRVVEQAATKTLFASPRHHYTAGLLASVPAVGDGRLREIKGMVP
                     SLWDLPKGCKFVDRCPAAQQVCRDEEPQLVQLGASKVRCHFPLPERTGDA"
     gene            2323..3267
                     /locus_tag="VMZ53_28910"
     CDS             2323..3267
                     /locus_tag="VMZ53_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HUS32570.1"
                     /translation="MSEVLLEAKAVTKHFGRLKAVSEVDLDVKAGETVGLVGESGCGK
                     STLGRTLLRLYEPTAGKILFDGKDISALSQRALRPMRREMQMIFQDPYASLNPRMTIG
                     AAIGEPIDIHGLAKTKQDREVRVLQLLAKVGLRPEMAKRYPHEFSGGQRQRVGIARAL
                     AVNPRFIVCDEPISALDVSIQAQIVNLLSDLQEADKLTYLFISHDLKIVQHVSDRVAV
                     MYLGRIVELADADTLYREPKHPYTEALLSAIPRVDAAAKRERIILQGDVPSPIDPPKG
                     CPFHPRCPVKDKVPACFTELPPLTIKKNGSRAACHVTE"
     gene            3290..6130
                     /locus_tag="VMZ53_28915"
     CDS             3290..6130
                     /locus_tag="VMZ53_28915"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS32571.1"
                     /translation="MQSRSGELAMRPLPAVLLDLHEDIATGRLTLRRGRVSKSVDLVN
                     GNPISTASSPRDETLGHFLVSSGVITEEQHKLGVQRSVSMGGKLGEALVAQGILTVEQ
                     LIEQLGKQARHKLVQALRWPQGAWRFEAGQQPVEGMQLRMLDVVLGGLRETAVEDLNR
                     LARLDGMSFELTERGKRLRHELKKSFGERALAILSAGGPIAEIEKAMGDRAQARVNVD
                     AMLMCDAIVSKGTQLGLGTVPAASQTLPGMGPASQRLAELVEGEDDDELYAMLFDEVD
                     DQKTRDGQAPLEFEEENVEAGNFEDSGVVSTVELQQASENRDKAVAARHAIVAEHSRI
                     QGADHYAVLLIDRDADADDIDAGYQIKLAMLEKKTQAVSDVGDKLKLEQIVDAYSTAR
                     IVLLDKQKRAAYDRELAGGELVAQPPSMNTELQFRVAEEMMAKAQWEQAVGTLKTVIA
                     SASGEADYHAALGWAVFNAGGAENNNDTALAALDHFNTALTINPDHPASHDYKGRVDA
                     ALRIDDAGAVFHLERALDLDPTRMDAVSTLESLLIARGELRRLERILKRLLFRLRGKG
                     GPAEVSAWTRLAKLYIDHLDDPAAGAAAIANARKLAPKDPEIGALASRTEGRRPNATD
                     PPRAGWREALSDASTGAALVNSTAAAGHADAAFLAASTMVALGTADDAMGALYDQNRV
                     RGVRLPARPLSREQWSVLRHKDDAVELGALLELVAPAVHALAPMTLADSDLDPGQLVA
                     DAELPAVFGKLKQQVAELLGVGAATPVYAKSELGFQIHVVACDPPVLAAGDEALTAPE
                     RADLVFRLARAMTFLWPGRAVGASRPGRALKAIVLAIVREASGSELGIEEPMARAAEK
                     AIRELDPTAHTQARASALRLLSRTGGGLNLSLWARSLSRTADRTGMLLCGDVPAAFAG
                     AKDMGDLDKDLVEFAYSAAHVNLRAQLGLSRG"
     gene            6152..6481
                     /locus_tag="VMZ53_28920"
     CDS             6152..6481
                     /locus_tag="VMZ53_28920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32572.1"
                     /translation="MEKPDHLRPVRTPRAAAVIALALGVVAFAVCAISQQDIWATPDW
                     RVSVPGLAVTSIAAIASVARREKSHALWIVGVALAAAATVLGWFLMVAIVIAVAAVLI
                     LILHSVM"
     gene            6489..7739
                     /locus_tag="VMZ53_28925"
     CDS             6489..7739
                     /locus_tag="VMZ53_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HUS32573.1"
                     /translation="MSATPDENELIYDWNSVEKVARLSPKRRIQFLDETLRDGIQSPS
                     VVDPSIDDKLRLVDLANELGIDTMDIGLPGAGKRAVEDVTIIAEHIRDAKLKVKASCA
                     ARTHINDVQAVVDISQKVGIEIEVLAFIGSSPIRQYAEDWDLTRMMKLSADAIDLARK
                     YNLPCAYVTEDTTRSRPEVLTKLFTNAVEHGAHRLIVCDTVGHVTPDGIRNLLKFTRN
                     VLDGMGRSDVGIDWHGHNDRGFGVVNSIFAIEYGADRIHGTAMGIGERVGNAALDQIM
                     MNLKLLGELPEHDLTKLVLWCKTASQATRVPIHPQYPLVGTDAFRTATGVHAAAIIKA
                     EKKGHNWLADRIYSGVPAGMFGKEQEIEVGHYSGESNVIYWLKKRGYEPTKDRVAAVL
                     NAAKRGNRVLTDDEIIAVIKSQPA"
     gene            complement(7736..8203)
                     /locus_tag="VMZ53_28930"
     CDS             complement(7736..8203)
                     /locus_tag="VMZ53_28930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32574.1"
                     /translation="MELTPELLEKMRAIGLRLDRGGTFWQGGSPVTHERLKQALLRWL
                     DVRTEDGRDIIKLDDKRYAYVDVDDAHLRAKSARWDGDHCFVLWDDDQEEELDYASLR
                     QAPDNAFYAKARGRLRGRIAGAAHHAVAERIVERGDSFVLEAAGKSWSIANAA"
     gene            complement(8483..9115)
                     /gene="tsaB"
                     /locus_tag="VMZ53_28935"
     CDS             complement(8483..9115)
                     /gene="tsaB"
                     /locus_tag="VMZ53_28935"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HUS32575.1"
                     /translation="MKILGIDSSTPIASAAVLDDGNLLASGESDAAGHRADLLVLIDR
                     VCREAGVAPLQLDAIAIGAGPGSFTGLRIGMATAKGIAFAAGKPLWGVSSLAALAYPV
                     DKPHVVAVLDARRGELYAGVYRRAPFALDGEERLIAPADLATFSQGGVTVGDYEGLAI
                     AARPTATAVAQLAFAGSRVDILVAGAPTYIRASEAEIRYPDGVPGALRKR"
     gene            complement(9112..10767)
                     /locus_tag="VMZ53_28940"
     CDS             complement(9112..10767)
                     /locus_tag="VMZ53_28940"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HUS32576.1"
                     /translation="MSVLYFFLLVGVLVVIHELGHFAAAKLLDVKVLRFSFGYGRPLV
                     RVTLGETEYQIAVFPLGGYVRILGIEREATPKADAGRSFAARPIWQRVVIILAGPAAN
                     LILPVIIYFAFFAGHTMLDAGVVGDVLEDGVAARAGIEPGDRIVEIDGKAIRYWEDIE
                     QIVQHSPNKELHLRISRNGKEFERYVTPVEDIVRQRDGKVSKQGRVGITHAPFVPLVG
                     VIDSQSPAARAGLRTGDLLISVDGQPVRNWSDVKHQLGKVARRSSIVYFRGTEMPGVP
                     QIQLLSPGFADLVPETQIDPELKRRTYTGLEHAEMFVAHVDPGSPADGAGLQPGDLIV
                     ALDDKPVMHWLDLDQRLQAEPAKTYKVTWKRALPDGSETKSAELTQVQRKELDDYGHT
                     VTRLVFGARNDVDRGQGAMTPIDGRFGYALGKALERTGDTISTMVTGFLKIVSGDKPA
                     DAFGGPYSVYRVASVSGSQGWDQFWLMIALISINLALLNLLPIPMLDGGHLLVFAIEG
                     ALRRPVAPKARERIQQAGLAIIIIIVILAAGNDIARYFFHQAP"
     gene            10862..>11618
                     /locus_tag="VMZ53_28945"
     CDS             10862..>11618
                     /locus_tag="VMZ53_28945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32577.1"
                     /translation="MLDGLDLTMDRLKTLYEQYFLGIQKQAPSFIHNDVERKLRDLTQ
                     LNVRNTGLRYRLATLQQKFGSYNSYWRRTLRQIENGTYIRNLAKIGREAAKNGSHIPD
                     EILAAMPKRMREQVLRDRDAALAIAKRRNQVEEGIGDDGLLELQDGANLPPEADDVAA
                     MISEPSVLKRELKTKTGAHVLSDDDGDLDFDAFFSAFEDDAPKPEKRPAPQQTQAITM
                     PIARMRPDTEQDLTLEGEPAPPTQAIPVMPRPSP"
BASE COUNT         2044 a         3800 c         3867 g         1907 t
ORIGIN      
        1 cgcgccggcg ccgacaccgc tctcgcccgc atcgcgcgcg ccgtcgaaga tgcgcaaggc
       61 agcaaggctg ctatcgcgcg cctcgccgat cgagtctcgg ccgtcttcgt gccgatcgtc
      121 ctcacgatcg ccacgctgac gttcgtcggc tggctcgcga ccggccacac cgcgactcaa
      181 tcactcgcga cgatgatcgc ggtgctcgtg atcgcgtgcc cgtgcgcgct cggcctcgca
      241 acgccggccg ccgtcgccgt cggcaccgca cgcggtgcag agctcggcct cctgttccgc
      301 ggcggtgacg cgctcgaacg cgcggcgtcg atcgacgtcg tgtgcctcga caagacggga
      361 acgctgacca ccggccaccc gcgcctcgtc tcgacgctcg acgacgatgt cctgcgcctc
      421 gccgcatctg cagagcaaca cagcgagcac ccgatcgctc gcgcgatcgt cgacgcagcg
      481 aaaacaaagg gactcgcgct cgcgcgcgcg gagaatgtga cggtcgaagc gggcggtggt
      541 ctgaccagcg tggtcgaagg ccgggcgatc aagatcggaa gccgcgcctt cgtcgcatcg
      601 gacctcgcgc gagggacaag cgggggttcg gcgatcgcga caagcgcggg ttcggcgatc
      661 gcgacaagcg gggtggcggc gatcgcgaca ggcgacgcga cctcattcat cgtgatcgac
      721 ggcgacgtgc ggggtgcgat cgaaatcgcc gatcttccgc gcgacgacgc ggcgacgacc
      781 atcgctgcat tgcgcggtct cggcgtcgag ccggtcatga tcaccggcga caagcgcgag
      841 gctgccgatc gcgtcgcggc ggaagtcggc atcacgcgcg tccacgccga gatcaagccg
      901 acgggcaagg ctgcgctcgt cgaggccgag aaggcgcggg gcaagcgagt cgcgatggta
      961 ggcgacggca tcaacgacgc gccggcgctc gccgcagcgg acctcggcat cgcgctcgcg
     1021 accggcacgg acatcgccgc cgcagccgcg gacgtcacgc ttctgcgcgg cggaattggc
     1081 gcgctgccga cggcactcac gctcgcgcgc gcgacgatgc gaaccattcg cagaaatctc
     1141 gcagccgcgt tcgtctacaa cgcgatctgc atcccgatcg ccgcggcggg cctgctctcg
     1201 ccggtgctcg cgagcgctgc catgtcgctg tcgagcgtga gcgtcctcgc ctcctcgctg
     1261 cgcctacgcc gcttccgcgc gtagggcggt gtcgcggcgc tacgaagtgt cgacgagcga
     1321 tccgcggtgc taaggtcgcc gtgcagtggg cgatgcagtc ctcgagatcc gcgacctcgt
     1381 gacggagttc cgcacggagc agggcgtggt tcgcgcggtc gacggcgtct cgttctcgat
     1441 tcccgcgcgc gggacgctcg ggctcgtcgg cgagtcgggc agcggcaaga gcgtcaccgc
     1501 attgtcgatc atgcggctcg ttgcttcacc gccggggcgc attgcgagcg gcgacattcg
     1561 ctatgcgggc aaagatttgc tcgcgttgcc ggccgaggag atgcgcgcga ttcgcggcaa
     1621 tcgcatcggc atgatctttc aggagccgat gacgtcgctc aatcccgtct ataccgtcgg
     1681 agagcaggtc ggcgaggcgg tgcggctaca tcagaagaag tcgaaggccg aggcgcgcgc
     1741 gatctcgatc gacatgttca agctcgtcgg cattccgtcg cctgccgagc gaatcgacgc
     1801 gtatccgcat cagctgtcgg gaggcatgcg gcaacgcgtg atgatcgcga tggcgctggc
     1861 gtgtaagccc gacctcttga tcgccgacga gccgacgacg gcgctcgacg tgaccattca
     1921 ggcgcagatc ctcgatctgc tgaagaagct gcagagcgag atgggcatga gcatcttgct
     1981 catcacgcat gacttgggcg tcgtcgcgga gacgtgcgac gaggtcgtcg tcatgtatgc
     2041 ggggcgcgtc gtcgagcaag ccgcgacgaa gacgctgttt gcctcgccgc ggcatcacta
     2101 cacggcgggg ttgctcgcgt ccgtgcctgc ggtcggcgac gggcggttgc gcgagatcaa
     2161 gggcatggtg ccgtcgctgt gggacctgcc gaagggctgc aagttcgtcg atcgctgtcc
     2221 tgcggcgcag caggtgtgtc gcgacgagga gccgcagctc gtgcagctcg gagcgtcgaa
     2281 ggtgcgctgt cactttccgc tgccggagag gaccggcgac gcgtgagtga ggtgttgctc
     2341 gaggcgaagg cggtcacgaa gcacttcggc cggctgaagg cggtcagcga agtcgacctc
     2401 gacgtgaagg cgggcgagac cgtgggcctc gtcggagaat cgggctgtgg gaagtcgacg
     2461 ctcgggcgga cgctgctgcg tctctacgag ccgacggcgg ggaagatcct gttcgacggc
     2521 aaggacattt cagcgctctc gcagcgcgcg ctgcgtccga tgcgtcgcga gatgcagatg
     2581 atcttccagg acccgtacgc gtcgctcaat ccgcgaatga cgatcggcgc ggcgatcggg
     2641 gagcccatcg atattcacgg gctcgcgaag accaagcagg atcgcgaggt ccgcgtgctg
     2701 cagctgctcg cgaaagtcgg gctgcggccc gagatggcga agcgctatcc gcacgagttc
     2761 tccggtgggc agcgtcagcg cgtcggaatc gcgcgcgcgc tcgcggttaa cccacgcttc
     2821 attgtgtgcg acgagccgat cagtgcgctc gacgtttcga ttcaggcgca gatcgtcaat
     2881 ctcctgtcgg acctgcagga agccgacaag ctgacctacc tgttcatctc gcacgacctg
     2941 aagatcgtgc agcacgtctc cgaccgcgtt gcggtcatgt atctcgggcg catcgtcgag
     3001 cttgccgacg ccgatacgct ctatcgcgag ccgaagcatc cgtatacgga ggcgctcctc
     3061 tcggccatcc cgcgcgtcga tgctgcggcg aagcgggagc ggatcatctt gcagggcgat
     3121 gtgccgagtc ccatcgaccc gccgaagggt tgcccgtttc atccgcggtg tcctgtcaag
     3181 gacaaggtgc cggcgtgttt caccgagctg ccgccgctca cgatcaagaa gaacggcagc
     3241 cgcgccgcct gccacgtcac agaataatcg cgctagaatt cccagactca tgcagtcgcg
     3301 ctccggggag ctcgcgatgc ggcctttgcc cgcagtgctc ctcgatctcc acgaagacat
     3361 cgccacggga agactgacgc tgcggcgcgg ccgcgtgtcg aagagcgtcg acctagtgaa
     3421 cggcaatccc atctcgacgg cgtccagtcc gcgcgacgaa acgctcggcc acttcctcgt
     3481 ctcgagcggc gtgatcaccg aggagcagca caagctcggc gtgcagcgct cggtctcgat
     3541 gggcggcaag ctcggcgagg cgctcgtcgc ccaggggatc ctaaccgtcg agcagctgat
     3601 cgaacagcta ggcaagcaag cgcgacacaa gctcgtgcag gcgctgcggt ggccgcaagg
     3661 cgcgtggcga ttcgaggccg ggcagcagcc cgtcgagggc atgcagctgc ggatgctcga
     3721 cgtcgtgctc ggtggcctgc gtgagactgc ggtcgaggat ctcaatcggt tggcgcgcct
     3781 cgacgggatg tcgttcgagc tcaccgagcg tggcaagcgc ctgcgccacg agctgaagaa
     3841 gtcgttcggc gagcgtgcgc tcgcaattct ttcggcgggc gggccgatcg cagagatcga
     3901 gaaggcgatg ggcgatcgcg cccaggctcg cgtcaacgtc gacgcgatgc tcatgtgcga
     3961 tgcgatcgtc tcgaagggca cgcaactcgg cctcgggacc gtgccggcgg cgagccagac
     4021 ccttcccggc atgggacccg cctcgcagcg tctggccgag ctcgtcgaag gggaagacga
     4081 cgacgagctg tacgcgatgc tgttcgacga ggtcgacgat caaaagacgc gtgacggtca
     4141 ggctccactc gagttcgaag aagaaaacgt cgaggccggt aacttcgagg attccggcgt
     4201 cgtctcgacc gtcgagctcc agcaggcgag cgagaaccgc gacaaggccg tcgctgcgcg
     4261 acacgcaata gtcgccgagc actcgcgcat tcaaggcgcg gatcactacg cggtgctgct
     4321 catcgatcgc gatgcggacg ccgacgacat cgacgcgggc taccagatca agctcgcgat
     4381 gctcgagaag aagacgcagg cggtcagcga cgtcggcgac aagctcaagc tcgagcagat
     4441 cgtcgacgcg tacagcaccg cccgcatcgt gttgctcgac aagcagaagc gcgccgcgta
     4501 cgaccgcgag ctggcaggtg gcgagctcgt cgcgcagccg ccctcgatga acacggagct
     4561 gcagttccgc gtcgccgagg agatgatggc gaaggcgcag tgggagcagg cggtcggcac
     4621 gctcaagacc gtgatcgcga gcgcgtctgg cgaggccgat taccacgcgg cgctcgggtg
     4681 ggccgtcttc aacgcaggtg gcgccgagaa caacaacgac accgcgctcg ccgcactcga
     4741 tcacttcaac acggcgctga cgatcaatcc cgatcatccc gcgtcgcacg actacaaggg
     4801 ccgcgtcgac gccgcactcc gcatcgatga cgccggcgcc gtgttccacc tcgagcgtgc
     4861 actcgatctc gatcccaccc gcatggacgc ggtctcgacc ctcgagtcgc tgctgatcgc
     4921 gcgcggtgag ctgcgcaggc tcgagcgcat cctcaagcgc ctgctgttcc gattgcgcgg
     4981 caagggtggc cccgccgagg tcagcgcgtg gacgcgccta gccaagctct acatcgatca
     5041 cctcgacgat ccggcggcag gtgctgctgc gattgcgaac gcacgcaagc ttgcgccgaa
     5101 ggatcccgag atcggtgcgc tcgctagccg caccgaagga cgtcgcccga acgcgaccga
     5161 cccgccgcga gcaggctggc gcgaggcgct ctccgatgcg agcactggtg cagcgctcgt
     5221 gaacagtacg gcggctgcag gccatgcaga tgccgcgttc ctcgccgcgt cgacgatggt
     5281 cgcactcggt accgccgacg acgcgatggg cgcgctctac gatcaaaatc gggttcgcgg
     5341 tgtcaggctc cccgcgcggc cactctcgcg cgagcagtgg tccgtcctcc gtcacaagga
     5401 tgatgccgtc gagctcggcg ccctcctcga gctcgtcgcg cctgccgtgc acgcgctcgc
     5461 tccgatgacg ctcgccgaca gcgatctcga tcctggtcag ctcgtcgccg acgcagagct
     5521 ccccgccgtg ttcggcaagc tgaagcagca ggtcgcggag ctccttggcg tcggcgctgc
     5581 aacaccggta tacgcgaaga gcgagctcgg ctttcagatt cacgtcgtcg cgtgcgatcc
     5641 gccggtgctt gccgcaggcg acgaagcgct caccgcgccc gagcgcgccg acctcgtgtt
     5701 ccgcctggca cgtgcgatga cgttcctctg gccgggccgc gcggtaggcg cttctcgtcc
     5761 gggccgtgcg ctcaaggcga tcgtgctcgc gatcgttcgc gaagcgtcgg gcagcgagct
     5821 cggcatcgag gaaccgatgg cgcgcgcggc ggagaaagcg atccgcgagc tggatcccac
     5881 ggcgcacacg caagcgcgcg cctcggcgct tcgcctgctg tcgcgtaccg gcggcggtct
     5941 caacctctcg ctgtgggcgc gctcgctgtc ccgtaccgcg gatcgaacgg gcatgctgct
     6001 gtgcggcgac gtgccggccg cgttcgcggg tgccaaggat atgggggacc tcgacaaaga
     6061 tctcgtcgag ttcgcctaca gcgctgccca cgtgaacctg cgggctcaac tcggtctatc
     6121 tcgcgggtag ttcgcaggct atacctccga cgtggagaag cccgaccacc tgcgtcctgt
     6181 gcgcacgccg cgagcagcgg ccgtgatcgc gttggcgctc ggcgtggtgg cattcgcggt
     6241 ctgcgcgatc agccagcagg acatctgggc gacgccggac tggcgcgtca gcgtgcccgg
     6301 cctcgcggtg acatctatcg ctgcgatcgc ctcggtcgcg cgccgcgaga agtcccacgc
     6361 gctgtggatc gtcggtgttg cgctcgctgc cgccgcgacg gtcctcggat ggtttctgat
     6421 ggtcgcgatc gtgatcgcgg tcgccgctgt tctgatactg attctgcatt ccgtgatgtg
     6481 aggaacctat gtctgcgacg cccgatgaaa acgagctgat ctacgactgg aactccgtcg
     6541 agaaggtcgc gcgcctctcg ccgaagcgcc gcatccagtt cctcgacgag acgctccgcg
     6601 atggcatcca atcgccgtcg gtggtcgacc cgtcgatcga cgacaagctc cgcctcgtcg
     6661 atctcgcgaa cgagctcggc atcgacacga tggacatcgg tctgccaggt gccggcaagc
     6721 gcgcggtcga ggacgtcacg atcatcgcgg agcacattcg cgacgccaag ctcaaggtca
     6781 aagcgagctg tgccgcgcgc acgcacatca acgacgtcca agctgtcgtc gacatctcgc
     6841 aaaaggtcgg catcgagatc gaagtgctcg cgtttatcgg ctcatcgccg attcgccagt
     6901 acgcggagga ctgggacctc acgcggatga tgaagctgtc cgcagatgcg atcgatctcg
     6961 cgcgcaagta caacttgccc tgcgcgtacg tcaccgagga caccacgcgc tcgcgccccg
     7021 aagtgctcac gaagctcttc acgaacgcgg tcgagcacgg cgcgcatcgc ctgatcgtct
     7081 gcgacaccgt cggtcacgtc actcctgatg gtattaggaa cctcctcaag ttcactcgca
     7141 acgtcctcga cggcatgggc cgcagcgatg tcggcatcga ttggcacggt cacaacgatc
     7201 gcggcttcgg tgtcgtcaac tcgatcttcg cgatcgaata cggcgcggat cgcatccacg
     7261 gcacggcgat gggcatcggt gagcgagtcg gcaacgccgc tctcgatcag atcatgatga
     7321 acctgaagct cctcggcgag ctgccggagc acgatctgac gaagctcgtc ctgtggtgca
     7381 agactgcatc gcaagcgacg cgcgtgccga ttcatccgca gtatccgctc gtcggcaccg
     7441 acgcgtttcg caccgccacc ggcgtgcatg ctgccgcgat catcaaagcc gagaagaagg
     7501 gccacaactg gctcgcagac cgcatctact ccggcgtccc ggccggcatg ttcggcaagg
     7561 agcaggagat cgaagtcggt cactactcgg gcgagagcaa cgtgatctac tggctgaaga
     7621 agcgcggcta cgagccgacg aaagatcgcg tggccgccgt gctgaacgcg gccaagcgcg
     7681 gcaatcgcgt gctcaccgac gacgagatca ttgccgtgat caaatcgcag ccggcttaag
     7741 cggcgttcgc gatcgaccag ctcttgccgg cggcctcgag cacgaagctg tcgcctcgct
     7801 cgacgatgcg ctctgcgacg gcgtgatgcg ctgcgcccgc gatgcggcct cgcagcctgc
     7861 cgcgagcctt cgcgtaaaac gcgttgtcgg gcgcttgccg aagcgacgcg tagtcgagct
     7921 cctcttcttg atcgtcgtcc cacaggacga agcagtggtc gccatcccag cgcgctgact
     7981 tggcgcgaag gtgggcatcg tcgacgtcga catacgcgta gcgcttgtcg tcgagcttga
     8041 tgatgtcgcg gccgtcttcg gtgcggacat cgagccagcg aagcagcgct tgcttcagcc
     8101 gctcgtgggt gaccggggag ccgccttgcc agaaggtgcc gccgcgatcg agacgcaggc
     8161 caatcgcccg catcttttcg agtagctcgg gcgttagctc catgaggaga ctatgggctg
     8221 atcaggagag ccgcgcgatg cggtctttcg tgaggagctt ttgcttcttg agcctcgaga
     8281 tctcggctct ctccgtggga gagagcgccc gtcgccgttc catatctttc aacagggcgt
     8341 cgaggcgacg gtgctctgca gtcagttcag cgagcaagac ttccttcgag acctcagtgg
     8401 tgctcaagcg tcctccaacc aataaccatc taactcgacg atacccccgg atcgggagcg
     8461 cggcaatcgc tggttcgatt ccctaacgtt ttcgtaacgc gccgggaacg ccgtccggat
     8521 agcggatttc tgcctcggac gcgcggatat aggtgggcgc accagcgacg agaatatcga
     8581 cacgcgatcc ggcaaacgcc aactgcgcca cagcggtcgc cgtcgggcgt gctgcgatgg
     8641 cgaggccttc gtagtctcca acggtgacgc cgccctgcga gaacgtcgcg agatcggccg
     8701 gcgcgatcaa acgctcttca ccatcgagcg caaacggcgc tcggcgatag acgccggcgt
     8761 agagctcacc gcgacgcgcg tcgagcacgg cgaccacgtg cggtttgtcg acgggatagg
     8821 cgagcgctgc gagcgacgac acgccccaca gcggtttgcc ggccgcgaat gcgatgccct
     8881 tggccgtcgc catgccgatg cgaagaccgg tgaacgagcc gggcccggcg ccgatcgcga
     8941 tcgcatcgag ctgcagtggg gcgacgccgg cttcgcgaca gacgcgatcg atcagcacga
     9001 gcaaatccgc gcgatgtccg gcagcgtcgc tctcaccgct cgcgaggagg ttgccatcgt
     9061 cgagcacggc agcgctggcg atgggagtcg agctatcgat gccgaggatc ttcacggagc
     9121 ttgatggaag aagtagcgcg cgatgtcgtt tcccgccgca aggatgacga tgatgatgat
     9181 gatcgcgagc ccggcttgct ggatgcgctc gcgcgccttg ggagcaacgg gccggcgcag
     9241 cgcgccttcg atcgcgaaca cgagtaggtg accaccgtcg agcatcggaa tcggcagcag
     9301 gttcaagagt gcgaggttga tgctgatgag cgcgatcatc agccagaact gatcccagcc
     9361 ctggctgccc gataccgacg cgacgcgata cacgctgtag ggaccgccga acgcgtcggc
     9421 aggcttgtcg ccgctcacga tcttcaagaa gccggtgacc atcgtgctga tcgtgtcgcc
     9481 ggtgcgttcg agtgctttgc cgagcgcgta gccgaatcgg ccatcgatcg gcgtcatcgc
     9541 gccttgcccg cggtcgacgt cgttgcgcgc gccgaacacg agacgcgtga cggtgtggcc
     9601 gtaatcgtcg agctccttgc gttgcacctg cgtgagctcg gcgctcttcg tctcgctgcc
     9661 gtcggggagt gcccgcttcc acgtgacctt gtaggtcttc gccggttcgg cctgcagccg
     9721 ctgatcgagg tcgagccaat gcatcaccgg tttgtcgtcg agcgcgacga tcaaatcgcc
     9781 aggttgcaga cccgcgccat ccgcgggcga gcctggatcg acatgcgcga cgaacatctc
     9841 tgcatgctcg agacccgtgt atgtgcggcg cttgagctct ggatcgatct gcgtttcggg
     9901 aacgagatcc gcaaagcccg gcgagagcag ctggatctgc gggacgcccg gcatctcggt
     9961 gccgcggaag tagacgatgc tcgaacgccg cgcgaccttc ccgagctgat gtttgacgtc
    10021 gctccaattg cgaacgggct ggccgtcgac gctgatcaat agatcgcccg tgcgcaaccc
    10081 tgcgcgtgcg gccggcgact gcgaatcgat cacgccgacg agcggaacga atggcgcgtg
    10141 cgtgatgccg acccgtcctt gcttgctgac cttgccgtcg cgctggcgaa cgatgtcctc
    10201 gacgggcgtg acgtagcgtt cgaactcctt gccattgcgc gagatgcgaa ggtgcagctc
    10261 cttgttcggc gagtgttgga cgatctgctc gatgtcttcc cagtagcgga tcgccttgcc
    10321 gtcgatctcg acaatgcgat cgcccggctc gatgcccgcg cgagcggcga cgccatcctc
    10381 gagcacgtca ccgacgactc ctgcgtccag catcgtgtgg cctgcgaaga acgcgaagta
    10441 gatgatgacg gggaggatca agttcgcggc tgggccggcg aggatgatga cgacgcgctg
    10501 ccagatcggc ctcgcagcga agctcctgcc cgcgtcggcc ttcggtgtgg cctcgcgctc
    10561 gatgccgagg atgcggacgt agccgccgag cgggaacacc gcgatctggt actcggtctc
    10621 accgagggtg acgcgcacga gcggccggcc gtagccgaac gagaaccgca ggaccttgac
    10681 gtccaacagc ttggccgcgg cgaagtgccc tagctcgtgg atgacgacga gcacgccgac
    10741 gagcaagaaa aagtacaaga ccgacatgaa accgagccgt ccgaggctat agcagagctg
    10801 ctatcctgta ggacaggtgg cttccaagtc cgaaagcgtc tccatacagc aagtcgagga
    10861 aatgctcgac ggcctggatc tgacgatgga tcgcctgaag accctgtacg agcagtactt
    10921 cctcggcatc cagaagcagg cgccttcgtt cattcacaac gacgtggagc gcaagctgcg
    10981 cgacctcacg cagctcaacg ttcgcaacac cggtctgcgc taccggctcg cgacgctgca
    11041 gcagaagttc ggttcctaca actcgtattg gcgacgcacg ctgcgtcaga tcgagaacgg
    11101 cacgtacatc cgcaacctcg cgaagatcgg gcgcgaggcc gcgaagaatg gctcgcacat
    11161 tcccgacgag atcctcgccg cgatgcccaa gcgcatgcgc gagcaagtgc tgcgtgaccg
    11221 cgatgctgcc ctcgcgatcg cgaagcgtcg caatcaagta gaagagggca ttggcgacga
    11281 cgggctgctc gagctgcaag acggcgccaa cctgccgccc gaggccgacg acgtcgcggc
    11341 gatgatctcc gagccgagcg tgctcaagcg cgagctcaag accaagaccg gcgcccacgt
    11401 gctcagcgac gacgacggcg atctggactt cgacgcgttc ttctccgcgt tcgaagacga
    11461 cgcgccgaag ccggagaaga ggccggcgcc gcaacagacg caggcgatca cgatgccgat
    11521 cgcgcggatg cgtccggaca ccgagcaaga cctgacgctc gaaggcgagc cggctccgcc
    11581 aacgcaagcg atccctgtga tgccgcgccc ttcgccgc
//
LOCUS       DATLBZ010000586         5670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7850791, whole genome shotgun sequence.
ACCESSION   DATLBZ010000586 DATLBZ010000000
VERSION     DATLBZ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7850791"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VMZ53_28950"
     CDS             complement(<1..718)
                     /locus_tag="VMZ53_28950"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HUS32578.1"
                     /translation="MDVGLVCDACSALTPIGVPQCARCGQPVALDPRPRRTSKPSGAN
                     GGAVAATSDAKSSGVACTKCGTNVPPNFKFCQICGTRVVVAQDFSVETRVGPTSASTP
                     VQAQGSTGAQAKQPGRSTLFFGGTMQQARARLTLIRGDGEDGVSFSLAGTDHLAGRGE
                     CPISFPDDPYLSPIHCNFRYANGALSVRDEGSLNGLYVRINGTVRVEPGTTVLVGEQV
                     LSVSAAKGFEDVPDTEGTYFS"
     gene            complement(821..4834)
                     /locus_tag="VMZ53_28955"
     CDS             complement(821..4834)
                     /locus_tag="VMZ53_28955"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS32579.1"
                     /translation="MRRLPLLLACALSALAPIAATSTTAHAQAGDWGVTRDPFDKTVI
                     ARYKGILAKNPHDAPALAKLLEMYRRYRTVDLLKEEYGKVLEKSAGDWSALVVLGRLA
                     RATGDDAKALEFLTKATGAKESDALAWMLIGELQKGAAKNKEARAAYDKALAHASGVE
                     MKKKALRALADLALTTGDNDGANAYFKQFLDLDPKNAQLWIERGDAMLAAGKRDVALE
                     SYTAAEKLLGTDKAKQVEVVARRGQALEGMGKDDEAIAEYRRAIKLSPKGYYLEVELT
                     GRVIDIYRRKQALPALLAQYEKEWPEGSRKHFEWNTLGKLYEETGSQDKAIAALKKAV
                     VSAPTELETQRRLIQLLENSGRDDEALAANEAVVRAAPGDARFQLELAERYWKRGEDK
                     KALETLSRLQTRFPNDPGVLSAIADFYMRWGKDDLAIGEYERLAKLEPDDPAHLVTLG
                     EQYFLKGDKKRAMEVWKRLKANNKATGWAKFGEVMVEHNFPTDALDAFNKAIELDGKN
                     PEFFKGRASLFESQKRYPDAIADWQKVLDLTGTKPSDRLARRDATRHQVQLITRSGVT
                     KEREFLAKWNKGFVDKNPPDVESGYFLVEYYSRKNMAPEQRATLEKMRTLVPEDWDLL
                     LDLVKSYRQARKFDEAVALLLELAKKQPARELQVYQEISEIKTEARKDDEAIEWAQKA
                     LAKSPTNAAAYERLGERYVEMQRFPDAIDAYEKTVKLDARNSKAAFALAQLYIQTGTP
                     MKATELFRNVLRNDTNDDVIARAGDQAIDLEEMTDTLGELEKVISPLSFMMAHKPVYR
                     RVLVKLYLRYVPRLVERSRHGNDEVKKAARVELDRIGARGLRPLLEALRDQKDVNQQR
                     IAVQVLGHLGNKGAAQPLVHMARQEPPKDQRHIGTLPESPDREARVDALVAAGRLGDA
                     GVLDDVLPLMDHPESSMREAATFTLGRSGDRKAVPALIKALGDRQARVQALACLGLAQ
                     IDDARVGPAVIGVLKDANAVDQVRGACAYAIGARKITAGVPALIATLDDNRGDAQRLS
                     AWALGQLAEAKALGPLARAYFARAGQSSDELVWAIGRVSGAGLAPSPLANTGDYPMRA
                     GNYNLAEAIREVPGDLPHPAPAAKLVVDHATDIAQGLTLALGEHRDVVVSVLADLDAA
                     PDRLALGALTPATNDAKVTAALATIAATIEPQVVTQLTSEDPKVRALAVSVLAKMDGG
                     KLTKAEGAIGKALTDPAEQVRASAMQSIAVLAKRRGTAPSGLVASLAKTLEAGAWADR
                     RVAALSLGAIGPSTDPAPLIKAAKDPSSFVREAVAVALGKVGGAKAQAALDELAKDEV
                     PQVRDAATAALGNRPKH"
     gene            complement(4914..5372)
                     /locus_tag="VMZ53_28960"
     CDS             complement(4914..5372)
                     /locus_tag="VMZ53_28960"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS32580.1"
                     /translation="MRRDWTRGRRKLGRRCQAGTILSHISPPEVEALHERRLGYRIPF
                     ESMVTSYVQDRPIRGLATNLSDTGVHMSTVSMVAPPPGMIVPLEIDLPGAGESIWASG
                     RICYRQDDRLASGLGVRFVAMAKSQARIVRDFCIEMRRKNLGSILARIRA"
     gene            complement(5488..>5670)
                     /locus_tag="VMZ53_28965"
     CDS             complement(5488..>5670)
                     /locus_tag="VMZ53_28965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32581.1"
                     /translation="HVDVDVDVDVDVHVHEHVSRGRGRGRGRARARARVTWTWTWTNT
                     GMGRARVTWTWTNTGT"
BASE COUNT          856 a         1944 c         1807 g         1063 t
ORIGIN      
        1 cggagaagta cgtgccctcg gtgtcgggaa catcctcgaa gcccttcgcg gcgctcacgg
       61 acaggacctg ctcgccgacg agaaccgtcg tgcccggctc gacgcgcacc gtgccgttga
      121 tgcgaacgta gaggccgttc agcgagcctt cgtcgcgaac gctgagtgca ccgttcgcgt
      181 agcggaagtt gcagtgaatc ggcgagaggt atggatcgtc ggggaacgag atcgggcact
      241 cgccgcgtcc cgcgagatga tcggttccag cgagactgaa cgagacgccg tcttcgccgt
      301 cgccgcgaat gagcgtgaga cgcgcacgtg cttgctgcat cgtgccgccg aagaacagcg
      361 tgctgcgtcc cggctgcttt gcttgcgcgc ccgtcgagcc ctgcgcttgc acgggcgtgc
      421 ttgcgctcgt tggaccgacg cgtgtctcga cactgaagtc ctgcgcgacc acgacgcgcg
      481 tgccgcagat ctggcagaac ttgaaattcg gcggcacgtt cgtcccgcac ttcgtgcacg
      541 cgacgcccga tgatttcgca tcggaagtcg ccgcgaccgc accaccgttc gcgcccgacg
      601 gtttgctcgt gcgtcgcgga cgcggatcga gtgcgaccgg ctgcccgcaa cgcgcacatt
      661 gcggtactcc gattggagtc aacgcgctac acgcgtcgca caccaagccg acgtccatcg
      721 cgtgtgattc tacccccttg cgaaacggtg cgtcaaagaa cgggggcgtg tctgatcggg
      781 tagttgagcg gtttcttcag ggagggcaga tccacgccga tcagtgcttg ggtcggttgc
      841 caagcgcggc cgtcgctgca tcgcgcacct gggggacctc gtccttggca agctcgtcga
      901 gggcggcctg cgcttttgcg ccgcccacct tcccgagggc cacagcgacg gcttcccgaa
      961 cgaagctcga cggatccttc gctgctttga taagcggcgc cgggtctgtg gacgggccaa
     1021 tcgcgccgag cgacagggcg gctacccggc ggtccgccca ggctccggcc tcgagcgtct
     1081 tggccagcga ggcgaccagc ccggacggcg cggtgcctct ccgcttggcg agcaccgcaa
     1141 tcgactgcat cgcggacgcg cgaacctgct cggcggggtc ggtcagcgcc ttgccgatgg
     1201 cgccctcggc tttggtcagc ttgccgccgt ccatctttgc gaggacggag acggcgagcg
     1261 cgcggacctt cggatcctcg ctcgtcagct gcgtgacgac ttgtggctcg atcgtcgcgg
     1321 caatcgtggc gagcgcggcg gtgactttcg cgtcgttcgt tgcgggcgtg agcgcgccga
     1381 gtgcgaggcg atcgggcgcc gcgtctagat cggcgagcac ggacacgacg acgtcgcgat
     1441 gctcgccgag cgcgagcgtg agtccctgcg cgatgtcggt ggcgtgatcg acgacgagct
     1501 ttgccgcggg cgcgggatgg ggcaggtcgc cgggcacttc gcggatggct tcggcgaggt
     1561 tgtagttgcc cgcgcgcatt ggatagtcgc cggtgttcgc gagcggtgac ggcgcgaggc
     1621 ccgcaccact gacgcgaccg atcgcccaca cgagctcgtc ggacgattgg ccagcgcgcg
     1681 cgaagtacgc acgagcgagc gggccgagag ccttcgcttc ggcgagctgt ccgagtgccc
     1741 acgccgagag gcgctgcgca tcaccgcggt tgtcgtcgag cgtagcgatg agggccggga
     1801 caccggcggt gatcttacga gcgccgatcg catacgcgca cgcgccgcgc acttgatcga
     1861 cggcgtttgc atctttgagc acgccgatga cggccggacc gacacgcgcg tcgtcgatct
     1921 gcgcgagacc gaggcatgcg agcgcttgca cgcgcgcctg gcgatcgccg agcgccttga
     1981 tcagcgcggg caccgccttg cgatcgccgg agcggccgag cgtgaacgtc gcggcttcgc
     2041 gcatcgagct ctcggggtga tccatcagtg gcagcacgtc gtcgaggacg cctgcatcgc
     2101 cgaggcgacc ggccgcgacc agcgcgtcga cgcgagcttc tcgatccggc gattccggca
     2161 gcgtgccgat gtgacgctga tccttcggcg gctcttgccg cgccatgtgg acgagcggct
     2221 gcgcagcgcc cttgtttccg aggtgaccga gcacctggac ggcgatgcgc tgctgattga
     2281 cgtccttctg atcgcgcagc gcttcgagca gcggacgcag accacgcgca ccgatgcggt
     2341 cgagctcgac gcgcgctgcc ttcttcactt catcgttgcc atgacgcgag cgctcgacga
     2401 gccgaggcac gtagcgcagg tacagcttca cgagcacgcg ccgatacacc ggcttgtgcg
     2461 ccatcatgaa cgacagcggc gagatcacct tctcgagctc gccgagcgtg tcggtcatct
     2521 cctcgagatc gatcgcctga tcgcctgcgc gcgcgatcac gtcgtcgttc gtgtcgttgc
     2581 gaaggacatt gcggaatagc tcggtcgcct tcatcggcgt gccggtctgg atatacagct
     2641 gcgcgagcgc gaatgcggcc ttgctgttgc gcgcgtcgag cttgaccgtc ttctcgtacg
     2701 cgtcgatcgc gtcggggaag cgctgcatct cgacgtagcg ctcgccgagg cgctcgtacg
     2761 ccgcggcgtt cgtcgggctc tttgcgagcg ccttttgggc ccactcgatc gcctcgtcgt
     2821 ccttgcgcgc ctcggtcttg atctcgctga tctcttgata gacctgcagc tcgcgcgcgg
     2881 gctgtttctt cgcgagctcg agcagaagcg cgacggcctc gtcgaacttg cgtgcttggc
     2941 gatacgactt cacgagatcg agcagcaggt cccaatcctc ggggacgagc gtgcgcatct
     3001 tctcgagagt ggcgcgctgt tcgggcgcca tgttcttgcg gctgtagtac tcgacgagga
     3061 agtagccgct ttcgacgtcc ggcgggttct tgtcgacgaa gcccttgttc cacttcgcga
     3121 ggaattcgcg ctctttcgtg acgccggagc gcgtgatgag ctggacctgg tgcctcgtcg
     3181 catcgcggcg cgcgagccga tcgctcggct tcgtgcccgt cagatcgagg accttctgcc
     3241 aatcggcaat tgcgtcgggg tagcgcttct gcgactcgaa cagcgatgcg cgccctttga
     3301 agaactcggg attcttgccg tcgagctcga tcgccttgtt gaacgcgtcg agcgcatcgg
     3361 tcgggaagtt gtgctcgacc atcacctcgc cgaacttcgc ccaacccgtc gccttgttat
     3421 tcgctttgag ccgcttccag acttccatcg cgcgcttctt gtcgcccttc aggaagtact
     3481 gctcgccgag cgtcacgagg tgcgccggat catcgggctc gagcttcgcg aggcgctcgt
     3541 actcgccgat cgcgaggtcg tctttgcccc agcgcatgta gaaatcggcg atcgcagaca
     3601 gcacgcccgg atcattcgga aaccgcgtct gcaggcgcga cagcgtctcg agagccttct
     3661 tgtcctcgcc gcgcttccaa tagcgctccg cgagctcgag ctgaaaccgc gcatcgcccg
     3721 gcgccgcacg cacgaccgcc tcgttcgcgg caagcgcttc gtcgtcgcgg ccggagttct
     3781 cgagcagctg aatgagccgc cgctgcgtct cgagctccgt cggtgcactc accacggcct
     3841 tcttcagcgc ggcgatcgcc ttgtcctgcg agcccgtctc ttcgtagagc ttgccgagcg
     3901 tgttccactc gaaatgcttg cgactacctt ccggccactc cttctcgtac tgcgcgagaa
     3961 gcgcgggcag tgcctgcttg cgacgataga tatcgatcac gcggccggtg agctcgacct
     4021 cgaggtagta gcccttcggc gagagcttga tggcgcggcg atactcggcg atcgcctcgt
     4081 cgtccttgcc catgccctcg agcgcttgcc cgcgtcgcgc gacgacctcg acttgcttcg
     4141 ccttgtcggt cccgagcagc ttctccgccg ccgtataact ctcgagcgcg acatcgcgct
     4201 tccccgcggc caacatcgcg tcgccgcgct cgatccacag ctgcgcgttc ttgggatcta
     4261 gatcgaggaa ctgcttgaag tacgcgttcg cgccgtcgtt gtcgcccgtc gtcagcgcca
     4321 ggtccgcgag cgcgcgcagc gccttcttct tcatctcgac gccgctggcg tgcgcgagtg
     4381 ccttgtcgta cgcagcccgc gcttctttgt tcttggcggc gcctttctgc agctcgccga
     4441 tcagcatcca cgcgagcgcg tcgctctcct tcgcgccagt cgccttcgtc agaaactcga
     4501 gcgccttcgc atcgtcgccg gtcgcgcgcg ccaaccgccc gagcacgacg agcgccgacc
     4561 aatcgcccgc gctcttctcg aggaccttgc cgtactcttc cttcaagaga tcgacggtgc
     4621 gataccgccg atacatctcg agcagcttcg cgagggccgg cgcgtcgtgc gggttcttcg
     4681 cgaggatgcc cttataccga gcgatgaccg ttttatcgaa agggtcgcga gtaacgcccc
     4741 agtcaccagc ctgcgcatgc gcagtcgttg acgtcgcggc gatcggcgcg agcgcgctga
     4801 gcgcacaagc gagcagaaga gggaggcgcc tcatgacctc ccccgatcat agccgctgca
     4861 tccgcccaat gcccgtcgga ccgggcttca tgacgaacgc ggagcccgcg caattacgcg
     4921 cggatgcggg ccaagatcga cccgagattc ttgcggcgca tctcgatgca gaaatcgcgg
     4981 acgatgcgcg cctggctctt cgccatcgca acaaatcgca cgcccaatcc cgacgcgagt
     5041 cgatcgtcct ggcgatagca gatccgcccc gacgcccaaa tcgattcgcc ggctcccggc
     5101 agatcaatct cgagcgggac gatcatgccg ggcggcggcg cgaccatcga tacggtcgac
     5161 atatgcacgc cggtatcgga cagattcgtg gcgagaccac gtatcggccg gtcctggacg
     5221 tagctcgtga ccatcgattc gaacgggatg cggtaaccaa gtcggcgctc gtgcaaggct
     5281 tccacctcgg gtgggctgat gtgggataaa atcgtaccag cttggcagcg gcgcccaagt
     5341 tttcgtcgcc cccgcgtcca gtcacggcgc acgtccgcat ccccgtcccg tccccgtcca
     5401 agcccgtccg cgtccccacg tccatgtcca cgtccacgtc cacgtccacg tccacgtccc
     5461 catccccgtg ttcgtccacg tccacgttca cgtccccgtg ttcgtccacg tccacgtgac
     5521 acgtgcgcgt cccatccccg tgttcgtcca cgtccacgtc cacgtgacac gtgctcgtgc
     5581 acgtgcacgt ccacgtccac gtccacgtcc acgtgacacg tgctcgtgca cgtgcacgtc
     5641 cacgtccacg tccacgtcca cgtccacgtg
//
LOCUS       DATLBZ010000587         4672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10095639, whole genome shotgun sequence.
ACCESSION   DATLBZ010000587 DATLBZ010000000
VERSION     DATLBZ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10095639"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VMZ53_28970"
     CDS             <1..210
                     /locus_tag="VMZ53_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32582.1"
                     /translation="VVAVTLETGRTHQIRAHLASIGHPIVGDDLYASGAVPGATSIAL
                     HASELSLPHPVTGVLLEPKTKRGPR"
     gene            complement(240..1622)
                     /locus_tag="VMZ53_28975"
     CDS             complement(240..1622)
                     /locus_tag="VMZ53_28975"
                     /inference="COORDINATES: protein motif:HMM:NF038123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HUS32583.1"
                     /translation="MNLRASCLCALALAACADKANDPFTDVDGDGVDDNDPDALHPFT
                     LRFENVAPYTVLKASVAYTQPGQTVGSNLAPGQFYEIRFTAGNNQNLSLATMLYESND
                     WFFGTDPAGIPLYTNGVPLSGDITSMLKLYDAGTEADEEPGVGSTTGVNQGTMSALGS
                     KDPNNLVRVLGDVVTLTNGAQFARPAIASMIKVTVTPGTQRDFLVRVQNVSTTDTLVT
                     SAGNRAITLTSTLWAVHCSPGIFFEEGKPVRANGFESLAEAGNAWTISDALRLERGIA
                     TPFSKGVYAIHQDGNPMFIENSVDYRFGLEKVAEDGDPSALVASYGQGLVGEVESYGA
                     FEAAEGPCHAGKSFEFQFKARQGDRLSLASGFMASNDWFLATPSEGMELFSGSLPRWG
                     EITTELRLFDLGTESDEELDIGSHVGTQQTSANSGNPDGNKNVRAIGRDKYDVPLTQH
                     VRVTLVPPTK"
     gene            complement(1776..4478)
                     /locus_tag="VMZ53_28980"
     CDS             complement(1776..4478)
                     /locus_tag="VMZ53_28980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32584.1"
                     /translation="MTGQLVLHYRILAELGQGGMGQVFRARDERLGREVALKMLPAEL
                     ASDADFQARLLREARAASALNHPGIVTLFDLQSFEGRTFLVMELVEGESLAKLARRGV
                     EWRKAVLLVIGVADALAAAHARGILHRDIKSDNIMITPSGQPKVLDFGLAKLRDLNAP
                     PGADQETGITANMKKAAAVSMNSMGLAATMRPVGGGPPAGPAGLTRAGQLLGTPAYMA
                     PECFEGVADARSEVFALGVVLYELLAGKRPFDRDSEMGTMSAIMLDEPEPLAGDVPPE
                     LNAVVRKALAKKPEERFADMASLATALRDLIPDRPSVAALTPPPSTTPPSRDIPAVPR
                     WWYAAGVAGLLVLIAGGWFVVSSTGRSSAKPPKPAAPTVVAPAAPTLEVTASKRITLD
                     PGCEEYPRQHPDGKRVVYDGVFEDDYEIMIVDLETNEHKRLTATKGWDYASALSPDGS
                     QVAFVHEDVEGRTLRVMPIDGSSPPRTLGAIVGYPAWSHDGALLVGDTSGHIVRREPA
                     SGAELVVGALPAGARLYHLVDVKDRGIALMWWTSSDADATSLGELDRSGHLRVIEEFA
                     TDYEGGLSPASNGHGYYATRKGASVGNQLLYREWGGEKPIGVPGVSPGAGIDVRSDGK
                     RLVFSTCTEREYVVRIAPNAAGDPAIISKGAWQDTNPSIVDAKSIVITSNRVGEAQGW
                     LVELDGKASPRAITPAGALGSAASPDGKLIAYATRSGLGIREIASGVDRSLTTDASDA
                     APAFTHDGAHIVFERTVDGVTNVYSVATAGGDARKLFAGEAPTASPKDDTIAFVTTAD
                     ATGTRHIMVGTLAGGATHELPKVEPGTWHRPKFAPDGKRLLVVRGYQQLVAVALDGST
                     PPRVVWTTTTGSVNTATWLRDGTGIIAAVGGYEGDLWLADGVFP"
BASE COUNT          739 a         1565 c         1563 g          805 t
ORIGIN      
        1 gtcgtcgccg tcacgctgga aaccggacgc acgcatcaga ttcgcgcgca cctcgcatcc
       61 atcggtcatc caatcgtcgg tgacgacctc tacgcgtctg gcgctgttcc cggcgcgaca
      121 tcgatcgcgc tgcatgcgag cgagctgtcg ctgccccatc ccgtgactgg cgtcttgctc
      181 gagccgaaaa cgaaacgagg gcctcgatga gaccctcgct gtagccgaat gctagacgct
      241 tacttcgtcg gcgggacgag cgtcacgcgg acatgctgcg tgagcggcac gtcgtacttg
      301 tcgcggccga tggcgcggac gttcttgttg ccgtccgggt tgccgctgtt cgcgctcgtc
      361 tgctgcgtgc cgacgtgcga accgatgtcg agctcttcgt cgctctccgt gccgaggtcg
      421 aacaggcgga gctccgtcgt gatctcgccc cagcgcggca gcgagccgga gaacagctcc
      481 atgccttcgc tcggcgtggc gaggaaccag tcgttcgacg ccatgaagcc cgaggccagc
      541 gagaggcggt caccttggcg cgccttgaac tggaactcga aggacttgcc ggcgtggcac
      601 gggccttcgg cggcttcgaa cgctccgtac gactcgactt cgccgacgag gccttgcccg
      661 tacgacgcga cgagggccga tgggtcgccg tcttcagcaa ccttctcgag gccgaagcgg
      721 tagtcgaccg agttctcgat gaacatcggg ttgccgtcct ggtggatggc gtagacgccc
      781 ttcgagaacg gcgtcgcgat gccgcgctcg aggcgcagag cgtcgctgat cgtccaggcg
      841 ttgcctgctt cggcgagcga ctcgaagccg ttcgcgcgga ccggcttgcc ctcttcgaag
      901 aagatgcccg gcgagcagtg gacggcccac agcgtcgacg tcagcgtgat cgcgcggttg
      961 cccgcggacg tgacgagcgt gtcagtcgtc gagacgttct ggacgcgcac gaggaagtcg
     1021 cgctgcgtgc ccggcgtgac ggtgaccttg atcatcgacg cgatcgcggg acgcgcgaac
     1081 tgcgcgccgt tggtgagcgt cacgacgtcg ccgaggacgc gaacgaggtt gttcggatcc
     1141 ttcgagccga gcgcgctcat cgtgccctgg ttgacgcccg tcgtcgaacc gacgcccggc
     1201 tcttcgtcgg cttcggtgcc ggcgtcgtag agcttgagca tcgacgtgat gtcgcccgac
     1261 agcggcacgc cgttcgtgta gagcgggatg ccggcggggt cggtgccgaa gaaccagtcg
     1321 ttcgattcgt agagcatcgt cgcgaggctc aggttctggt tgtttccggc ggtgaaccgg
     1381 atctcgtaga actggcccgg cgcgaggttg ctgccgaccg tctggcccgg ctgcgtgtac
     1441 gcaacgctgg ctttgaggac ggtgtacggc gcgacgttct cgaagcggag cgtgaacgga
     1501 tgcagggcgt ccgggtcgtt gtcgtcgacg ccatcaccat cgacgtcggt gaaggggtcg
     1561 ttcgctttgt cagcacacgc ggcgagcgcg agcgcacaca gacaggatgc acggaggttc
     1621 atggaggtca ggtcctttcg gtgtcggggg cgtgcgttac aaactgcgcg ccagcctgag
     1681 agacgccgat caaggagttg gagcgtgcgt gcgcgccgac gacgcggtct tacgacggcg
     1741 caggcgcaca gtggtgcgct ccctgtacgc acgctttacg gaaagacgcc gtcggcgagc
     1801 cagagatcgc cttcgtaacc gccaaccgct gcaatgatgc cggtgccgtc gcgcaaccac
     1861 gtggccgtgt tcacgctgcc ggtggtcgtg gtccacacca cacgtggtgg cgtgctgcca
     1921 tcgagcgcga ccgcgacgag ttgctggtag ccgcggacca cgaggaggcg tttgccgtcg
     1981 ggcgcgaact tcgggcgatg ccacgtgccc ggctcgacct tcggcagctc gtgggtggcg
     2041 ccgccggcaa gcgtgccgac catgatgtgg cgcgtgccgg ttgcgtcggc ggtcgtgacg
     2101 aacgcgatcg tgtcatcctt gggcgaggca gtgggcgctt cgccggcgaa caacttgcga
     2161 gcgtccccgc ccgcagtcgc gacgctgtac acatttgtca cgccatcgac ggttcgctcg
     2221 aacacgatgt gcgctccgtc atgcgtgaat gcgggcgcgg catcggacgc gtcggtggtc
     2281 agcgagcgat cgacgccgct ggcgatctcg cgaatgccga ggcccgagcg cgtcgcgtac
     2341 gcgatgagct tgccgtcggg agacgcagcc gagccgagcg caccggcggg cgtgatcgcg
     2401 cgcggcgacg ccttgccgtc gagctcgacc agccaccctt gcgcttcacc cacgcgattc
     2461 gacgtgatca cgatcgactt cgcgtcgacg atcgacgggt tcgtgtcctg ccacgcgccc
     2521 ttcgagatga tcgccgggtc gcccgcggca ttcggcgcga tgcgcacgac gtattcgcgc
     2581 tccgtgcacg tggagaagac gagccgcttg ccatcgctgc gcacgtcgat gcccgcaccc
     2641 ggcgacacac ccggcacgcc gatcggcttc tcgccgcccc actctctata gaggagctga
     2701 ttgccgaccg atgcgccttt gcgtgtggcg tagtagccgt ggccgttgct cgcaggagag
     2761 aggccgcctt cgtaatccgt cgcgaattct tcgatgacac gcagatggcc gctgcgatcg
     2821 agctctccaa gcgacgtcgc gtcggcatcg ctcgatgtcc accacataag tgcgatgccc
     2881 cgatctttga cgtcgacgag atgatacagg cgcgcacccg ccggcagcgc accgacgacg
     2941 agctcggcac cacttgccgg ttcgcgccga acgatgtgcc cgctcgtatc gccgacgagc
     3001 agcgcgccat cgtgcgacca cgcgggatag ccgacgatcg ccccgagcgt gcgcggcggc
     3061 gagctgccgt cgatcggcat cacgcgcagc gtgcgtccct cgacgtcttc gtgcacgaac
     3121 gcgacctgcg aaccatcggg cgagagcgca gacgcgtagt cccagccctt cgtcgccgtc
     3181 agtcgcttgt gctcgttcgt ctcgaggtcg acgatcatga tctcgtagtc gtcctcgaac
     3241 acgccgtcat agacgacgcg tttgccgtcg ggatgctggc gcggatactc ctcgcatccc
     3301 ggatcgagcg tgatgcgttt gctcgccgtc acttcgagcg tcggcgcagc gggcgcgacc
     3361 acggtgggcg cagcaggctt gggcggcttc gcgctcgagc gccccgtgct gctcacgacg
     3421 aaccatccgc cggcgatcag cacgagcaat ccggcgacac cagccgcgta ccaccaccgc
     3481 ggcacggccg gaatatcgcg tgacggcggc gtcgtggacg gcggcggcgt gagcgctgcg
     3541 accgacggcc gatccggaat cagatcgcgc agcgccgttg cgagcgatgc catgtcggcg
     3601 aagcgctcct cgggcttctt cgcgagcgcc ttccggacga cagcattcag ctcgggcggc
     3661 acatcaccgg cgagcggctc gggttcgtcg agcatgatcg ccgacatcgt tcccatctcg
     3721 ctgtcgcgat cgaatggacg cttgcccgcg agcagctcgt acagcacgac accgagcgcg
     3781 aacacttcac tccgcgcatc ggccacaccc tcgaagcact cgggcgccat ataggccggc
     3841 gtgccgagca gctgtcctgc gcgcgtcagt cccgcgggac ctgcgggcgg cccaccacct
     3901 acaggacgca tcgtcgcggc gaggcccatc gaattcatcg agacggccgc ggccttcttc
     3961 atgttcgcgg tgatgcccgt ctcctgatca gcaccgggcg gtgcattcaa gtcgcgcagc
     4021 ttcgcgaggc cgaagtcgag gacttttggc tgaccgctcg gcgtgatcat gatgttgtcg
     4081 gacttgatgt cgcgatgaag aatcccgcgt gcgtgcgcag cggcgagtgc atccgcgaca
     4141 ccgatgacga gcagcaccgc tttgcgccac tcgacgccgc ggcgcgcgag cttcgccaga
     4201 gactcgcctt cgacgagctc catcacgagg aacgtgcgcc cctcgaagct ctgcagatcg
     4261 aacagcgtga cgatgcccgg atgattcagc gcgctcgccg cccgcgcctc gcgcaggagc
     4321 cgcgcctgga aatcggcgtc gctcgcgagc tcggcgggca gcatcttgag cgcgacctcg
     4381 cgtccgagcc tctcgtcgcg cgcgcggaag acctggccca tgcctccttg gccgagctcc
     4441 gcaaggatgc ggtaatgcag cacaagctgt ccggtcaccc ggcgacatcg taactgtgat
     4501 cgcccacagc tgtcgtccgc cggtgcgtgc gacacccacc tgccggccgg gagatcgtcg
     4561 attaccgacg cctgcccgag cgctcaccaa caagaacttg aacgtttgcc ccactcaggg
     4621 aaacagttct atgtggccct tcggaggatc gtcctggcgc tcgccgtcag tg
//
LOCUS       DATLBZ010000588        17779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10878011, whole genome shotgun sequence.
ACCESSION   DATLBZ010000588 DATLBZ010000000
VERSION     DATLBZ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17779
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10878011"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /gene="nadA"
                     /locus_tag="VMZ53_28985"
     CDS             <1..184
                     /gene="nadA"
                     /locus_tag="VMZ53_28985"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HUS32585.1"
                     /translation="LIPAPPEDESCSCNKCPFMRKNTLEKLYLCLRDLQPQVDVPEAV
                     RLKALTPIQRMLDMSV"
     gene            181..957
                     /locus_tag="VMZ53_28990"
     CDS             181..957
                     /locus_tag="VMZ53_28990"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HUS32586.1"
                     /translation="MKRVALLVLLAACSDTKPATPPAALPTPPPGKPAATVIPATTKL
                     LVTGIVDDWTATNATLRVWRRDGAAWKPIGEAWPAVVGRTGTAWGIGLHGSPNREGPI
                     KREGDGKSPAGVFAIDAAFGVADDPPKGAKLHYESTARGDWQCVDDPASDHYAQIVDR
                     KQVPSDWHSHEEMLRNDSLYTWVIDVAHNPEHVAGKGSCIFLHVWGGKDTTTVGCTAM
                     EEPRLASLLAQLDPADHPLYVLLPRAEYTALASTWGLPPL"
     gene            936..1493
                     /locus_tag="VMZ53_28995"
     CDS             936..1493
                     /locus_tag="VMZ53_28995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32587.1"
                     /translation="MGFASALALLLALSACGSQSPSTPEARLAAYLATHEVTKLDRES
                     FHQIIVEPFRDLYDDYAAQPIPERPISGPVTVRRHFANDPSLTPAQGRLRWMLPVMYP
                     SYAAAGTVFVEIHGEWRSLAGLDEVMLARVRTLDATCADRLSLAGPVGNCTNAGWVIA
                     DAALRHDTARFARACQLAASACTGR"
     gene            1507..1962
                     /locus_tag="VMZ53_29000"
     CDS             1507..1962
                     /locus_tag="VMZ53_29000"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUS32588.1"
                     /translation="MNWKTILVPHDFSSSANHAAAIARDEAKAHGSKLLLLHVIQLPG
                     QLQPDVAIIPEPTGAPISMREYAVKQAEAHLEDLAARLAKDGVNAQTFLRIGNPVDEI
                     LTFVVENHVDLIVMGTHGRSGVAKMLAGSVTERVVRASTVPVLTTRHPE"
     gene            1962..2828
                     /locus_tag="VMZ53_29005"
     CDS             1962..2828
                     /locus_tag="VMZ53_29005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32589.1"
                     /translation="MRATLALLLAACGSSKSVPQTTPTTTTTPTISTTPGSTGPAVYP
                     PPDLSPPTTGLAVGIAAHVVIDDRGSRLFVRKANGIDILALDSGKKTGRIDIGWDGEL
                     WPAGAHVLALRIAPSAVLEVALVDPATNKIAATCSGVVAVPANSQISRVDEFTTHAGT
                     TYLQWETSPPPRRMGGAAVSDEQMAAITSEFRAAYACGLFAIHTNDSRCTLDKATPQE
                     AGLEACGSRSMPWNRYLPSPIGKLALRVDRSSAGKAGLLVDTDTLVISELGGSELWKL
                     PIETRAIEPPPP"
     gene            complement(2825..3322)
                     /locus_tag="VMZ53_29010"
     CDS             complement(2825..3322)
                     /locus_tag="VMZ53_29010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32590.1"
                     /translation="MRKLILLALVTAACTPRPKAPTKPTEYVCEGYSLMRDGSEVKAS
                     DGNLVGRLSWRDSTGEHFVAWPTTPTDREAIEIVIPSDPRQDAMHRIYDTTFGSSTAD
                     WRLKSRQTCTARDGYSDILTRYIKGETLDDLTTSLALDSNDETRVLLRRAMASLNHRY
                     WRDGH"
     gene            complement(3424..5169)
                     /locus_tag="VMZ53_29015"
     CDS             complement(3424..5169)
                     /locus_tag="VMZ53_29015"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS32591.1"
                     /translation="MTSSNVEETASRLARFGDDGEGPGTVYLLVVDNDTSSIFHLPRS
                     GAIVIGRDKDVELRVQHASVSRRHATVRVDDGVVRAADLGSHNGTRVNGEPVTESRVL
                     ASGDVLTVGDVVLVVHFSAPSLVARTAYAEPGWKRRLAEELVRAITFKRSLVVAVIAG
                     AAAQVAELGNALRLIDVLGTADDGHVLVLLPEIARADAHAVIERALAGIPGARAGMAE
                     CPTDACDADTIVLAARSALRRAKPGTVAAAAETGQWIELGTRRVLVGDPAMSRVFALL
                     ERVAATELAVLISGETGVGKENAAYAVHHWSKRSGPFIAVNCAALGPETLVDAELFGY
                     EKGAFTGATAAKAGLFENASAGTVFLDEVGELPLALQAKLLRALESKRITRLGEVRER
                     AIDVRVVSATNRSLEAEVAAGRFRQDLFFRLSGATVIVPPLRDRRCEIPLLARAFLDE
                     VQARSGRPRMTVSPEAMQTLLTYAWPGNVRELKHTMEYAMAAAPDETIEPYDLPERLG
                     GTASVGAAATPPDGVPASDGNVTFRPISDELRDLERRRMAEALAAAGGVKTRAAQLID
                     MPIRTFTLKLKQYKL"
     gene            complement(5166..8510)
                     /locus_tag="VMZ53_29020"
     CDS             complement(5166..8510)
                     /locus_tag="VMZ53_29020"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32592.1"
                     /translation="MPSWVGRGMGDAVRDGEVRAADPEDTIDANDRAREAPVAAIDVV
                     VPARDYAQLTTVERRHYLVTGELATGGMGRVLEARDLRLGRDVAIKELLPKNRDAARR
                     FEREARITARLQHPSIIHVYEAGVWPGGEPFYAMPRVSGRSLDKVVGEKQTLAERLAL
                     LPHVIAVADALAYAHNENVIHRDLKPANVLVGEYGETVVIDWGLAKDLGAPSDPKESL
                     ALRLRATAEETASGSIVGTPAYMPPEQARGEAVDQRADVYALGALLYKLLAGVPPYTG
                     EHSRDVLELVKAGPPRPVHEVVPDAPPELVAIVNQAMQRDPDDRYITASALAQDLKRF
                     ETGQLVGAHRYTVSQLVVRWLRRYRLAVGVGAAALVAMVVLGVISVSRIVTEKHRAEA
                     AQAKAQAGRIELLQEHGRSELLAGRSGAALAYLVGASEDGNRGGARGFLIADAMRPFD
                     AEIAHIEVGKGNVTLAVRDDGTELVTSGAGDVVLWSADGKQIRSFGGRGTSRVVAIDQ
                     TGARVVAAGDDGVAHVWARDGSLVADLQGGTGPINAAVFSRDGKRLVTGGEDGSVIVW
                     DLATKAKYVSASDTCHSAPVVSLELSASGSEVLSASADATACVLSVVDGSVQKQLRGH
                     RGRINAATWSSDGKSVITASDDATARVWNVDTERTVPIAKPSVAPLAHMQGAGVNVAL
                     LLDEQRAVTAGADGVVYIWSLPAELPVGEAPPAARVVRRFVAHTGSVVAAAVSADGRF
                     MATGGYDALAKIWDLQTGQQIATFEHADVVTSVRFVGGTTLATASRDGTVRLWNTATI
                     KIRHELDSVIHAIAIAHDGTVAVATDDSRVTLVRAGDTAPLAGHLGRVFAVAFAPAKA
                     LLVSGGEDAESIVWDTDKRVPVARLAIPTPVRELAISGDEDTVVALAGDHVELWSIAS
                     KSRKLVLPSNAKRLDAVAINPRDGSFAAVGASGALVQWSASGEKLVEISDAARPYSAL
                     AFSKNGTILVAAGPGLADVWRVHDGKLSFELALDGATGIVRAAAVTDDSSMIVTAGDD
                     GRAYVWDAERGKRLGSRDYHERPVSSVRIDGDTLWVASEDGTLDAWDIHVDTRSAAEL
                     AAAMKAKHVPEYLDADTVVRAEGNTR"
     gene            8620..9735
                     /locus_tag="VMZ53_29025"
     CDS             8620..9735
                     /locus_tag="VMZ53_29025"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HUS32593.1"
                     /translation="MRSDGFTDAQWTALQKQYTLESPPEMCSGPCSLGFPLGQRLFFE
                     PALSGNGEVSCATCHAPTGWYVDPRRTDVSFGTVKWTQHNTISLVNLAYKRTFTWAGQ
                     CGDHSCLSPNDVIVDIALPKAMTSTRTLVTQTIENNPEYLYLYRSSFSLGDPDVVANV
                     ATSLHAYMRALVSVDAPFDAYIAGNDSALSDKARRGFALFVGKAMCAECHRGPMFTDN
                     TIHVTGVAQRGEHAPAMDAGAPGFFTPGLRNIDKTAPYMHDGSLANLADVIDFYRRGG
                     DTSGFSGDKDPLMQPVDITDDEARDLEAFLHALTGQALPETLTMDLRSYPPPPTCQLT
                     TCGTACVDTNDDAMNCGGCGKVCPVNKPVCAGGVCGH"
     gene            9723..11111
                     /locus_tag="VMZ53_29030"
     CDS             9723..11111
                     /locus_tag="VMZ53_29030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32594.1"
                     /translation="MRALAIVALLAWPLVASADTEPAHDEAEDDEAPVITDDDGDEPT
                     PAELADEVDDLAQRQKKLERAQRTNDETRRELNRMKYLMRFINVYVDVGAFAVAGDGS
                     GIRSDVGHVYYPEYMGKVPGQWVFMGDPLSTAINSQGEPSDTSSSREIAVDTVNSEGH
                     PSVIVNAVGLSIGKDLTHDIAVASLTQLMPRVTSNVLDVQLAEILYRHPVGDGNLRVS
                     AGKIDSVLGIEYRAQDAPVRLGVSPSLICRYTCGRPLGVRAQLVEGAISVSATMTNGN
                     NFQALFEHDSKLKANAAPTASAHVQYLLPVGQGLEVGVSGALGPQDNQPHIDVTQWHI
                     GFDAKLTDLHGFDAAAEYVQGEQEGQTTGMAECDAAPCLTYKGAYLLVARRVQAWVTP
                     YARIDWRDAVHTDGARFVYESHTVRGTVGAHFEMTSRILAKVEYTWNHELGNIPQFAN
                     DIVTSSLVVATD"
     gene            11111..11821
                     /locus_tag="VMZ53_29035"
     CDS             11111..11821
                     /locus_tag="VMZ53_29035"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32595.1"
                     /translation="MRRAYVPLVALLPLLLGAEGATPPAGGSIAGSVVTVKDGKPAKR
                     DDVYVYLQRVGRSKNRELPGKGVRKEIHQKGKAFEPHVVVIPVGGVVAFPNDDKEDHN
                     VFSPTDPGFDLGRYTTDKKGKPHTFEDADEFDIFCDIHPQMWAKVKVVESEYIAQVVD
                     GKFSFEGVAPGTYKVIAWTSDSAEVKSDKVVVTAGRSITLPNALHLQLTVASGCHDRK
                     DGTPYDAKYGRSSRCPQK"
     gene            11915..13240
                     /locus_tag="VMZ53_29040"
     CDS             11915..13240
                     /locus_tag="VMZ53_29040"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HUS32596.1"
                     /translation="MRNTIWCVVLVAACGDSTSVAIDARPDSPDVDAADPLALTTEEL
                     AAAHTLSPLPAVPADTTNAYADNAAAARLGQMLFFDKSYAGPLVVGDTGTNGGLGKPG
                     EAGKVSCASCHAAGSEAIDDRRSIPNATSLGTDRGTRNALGVVNSSFYKWTNWGGRFD
                     SQWSLPMAVAENAKIMDSTRLAIAHMIYAKYRTEYNAIFEPDLDPDLDPAASNAARFP
                     ATGKPGQTNFDNMAAGDKTIINRIFANYGKALAAYVRTLVSRDAPFDRFVAGDHSALT
                     APQVRGLKLFISKKCIGCHSGPNFSDDEFHALASAPVTDMGRFADLPVLLASTANVNS
                     ALSDDTNTHKLDGLAAIDSMKGQFRTKSLRNVAGSGPFMHGGTLATLGDVVDFYSQGG
                     GDVGASGVTKDTRMTPFALTAGEKADLVAFMESLSGAPVPASAIVDISK"
     gene            13330..13785
                     /locus_tag="VMZ53_29045"
     CDS             13330..13785
                     /locus_tag="VMZ53_29045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32597.1"
                     /translation="MERPRTRSRRNLPPIRGGEIMHSSPTNPGALASAAWCAERALFP
                     KDPNWSVEIALHADEWRFTIEIYAQEWGFAVRHLERTSWIRVTDVAFVHGSDDFQLLR
                     NTPHLGSIGELIASIEIDQRIVFGRANAIVQSSFGGEDVIRAWVRSLGR"
     gene            complement(13774..14082)
                     /locus_tag="VMZ53_29050"
     CDS             complement(13774..14082)
                     /locus_tag="VMZ53_29050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32598.1"
                     /translation="MRAWIVEELRLMTWIGELDLSSIANPADVPTQLDLLIVGSDGLS
                     ASDLDRVASRRKSTPVIAVGSSNMQAERTFGPKLTSRELKQAIRELVFAPAARVASPA
                     "
     gene            14348..15379
                     /locus_tag="VMZ53_29055"
     CDS             14348..15379
                     /locus_tag="VMZ53_29055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS32599.1"
                     /translation="MLETTRDEDLNLRVRKNAVLRAWARQTPRGSLMVGPVDVVLDEL
                     VNFVVGEDGSAESLSAAVEGHVAGRFRDGFTVDAVIGEYALLRRCIHRELGNRRFDHP
                     VDVAIGYAAKHYLTMREELRERFIGVLAHDLRSPLACVTMGTEMLGVDERSEHERSLL
                     KLVLESSDRMRRMVDEILGWAKRSPVEGFPAAQRFDDMAEILQAVAQETQLVHGVDAI
                     ELETTGDLRAELDRDRVHQAVVNLVRNAIEHGTGSAHVSASEADGGQTIVLVVRNRGA
                     LRSPNASDISQPFERRKRATSSRGLGLYIVDQIARSHGAGLDMTSNLDETAVTIRWPT
                     RRIVDGPSF"
     gene            15480..16172
                     /locus_tag="VMZ53_29060"
     CDS             15480..16172
                     /locus_tag="VMZ53_29060"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HUS32600.1"
                     /translation="MGPSIAFQRLTSVRLVDRLNQETSSLHAEVDEELVKQPVSARGY
                     QRFLARTYGFVAPLERAITTTPAIEKYVDLRRFNKEELLRRDLLALHYTNHQIDGLPQ
                     CSVPLFNTAAEALGWGYFIERSTLAHGSAFRAVASNIPGEVAFASSYLKCYFGAVGEM
                     WRAFGNTLDQIAADTDTDLVIEAARTAFKFYQRWREPDRESTQPDVTSTSGSLRLTAI
                     EQLAQDDDSTLS"
     gene            complement(16180..>17779)
                     /locus_tag="VMZ53_29065"
     CDS             complement(16180..>17779)
                     /locus_tag="VMZ53_29065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32601.1"
                     /translation="EVAVKTLRSDATDEAAPRLLREAWITGALEHPNIVPVHDLGSDE
                     RGRPLLVLKRIEGEPWHPKSRDLEDNLATLMQVCHAIAFAHSRGIVHRDLKPDNIMIG
                     RFGEVYVLDWGIAVATKDDGSGRLPLAAESREVAGTLAYMAPEMLGGAAVTEQTDVFL
                     LGAILYEIIEGRPPHLLESIGASITSIALCEPSLSPDVPAELVAICRRAMAREAAQRY
                     QTADELRLAVAAFLERRTSAQIAHESHEKLRELETQLAAKDATRVTMYQLFGQCRFGF
                     LAALRAWPENPEAREGLDRALAAMATHELDHGDARAAAVLIAELSTPRPALADRLATV
                     ERDATEKRKRLERFELDQDVQAGQLTRSFFLTLLGILWSAIPLAGNVIDHVSHAQSFG
                     APITMLVGIVVFGWWARDSMTKTALNRGVLMTVFIGVVLHAAVEAIAWAGGLELWQTH
                     LLALAMWTGASAVGSVFLDRAMLAPVVVFVGTMLVTVLAPAHRYWAMSVACLSMFSTA
                     IYVTIRERRGRRVYGIADVLRRND"
BASE COUNT         3101 a         5987 c         5725 g         2966 t
ORIGIN      
        1 gctgatcccg gcgccgcccg aggacgagag ctgctcgtgc aacaagtgcc cgttcatgcg
       61 caagaacacg ctcgagaagc tctacctgtg cctgcgtgat ctgcagccgc aagtcgacgt
      121 gcccgaggcg gtccgcctca aagcgctcac gccgatccag cgcatgctag acatgagcgt
      181 gtgaagcgag tcgcgctgct ggtgctgctc gccgcgtgca gcgacacgaa gccagcgacg
      241 cctcccgcgg cattgccgac gccgcctccg gggaagcccg cggcaacggt gatcccggcg
      301 accacgaagc tcctcgtcac gggcatcgtc gacgattgga ccgcgacgaa cgcaacgctg
      361 cgcgtatggc gacgcgacgg cgcggcatgg aagcccatcg gagaagcgtg gccggcggtc
      421 gtcggcagga cgggaacggc gtggggcatt ggcctgcatg gctcgccgaa tcgcgaaggt
      481 ccgatcaagc gcgaaggcga cggcaagagt cctgctggtg tgttcgcgat cgatgccgcg
      541 ttcggcgtgg ccgacgaccc gccgaaaggc gcgaagctgc actacgagtc gaccgcgcgc
      601 ggcgactggc aatgcgtgga cgatccggcg agcgaccact acgcgcagat cgtcgatcgc
      661 aagcaagtgc cgtccgactg gcactcgcac gaagagatgc tgcgcaacga ctcgctctac
      721 acgtgggtca tcgacgtcgc gcacaatcct gagcacgtcg cgggaaaagg cagctgcatc
      781 ttcctgcacg tgtggggcgg caaagacacg accacggtcg gctgcaccgc gatggaggag
      841 cctcgcctcg cctcgctgct cgcgcagctc gatcccgctg accatccgct ctacgtgctg
      901 ctcccgcgtg cggagtacac cgcgctcgcg tccacatggg gtttgcctcc gctctagctc
      961 tgctgctcgc gctctccgcg tgcggctcgc agtctccgtc gacgccggag gcacgcctcg
     1021 ctgcctatct agcaacgcac gaggtcacga agctcgatcg cgagtcgttt catcagatca
     1081 tcgtcgagcc gttccgcgac ctctatgacg actacgctgc gcagccaatt cccgagcgtc
     1141 cgatttccgg acccgtcacg gtgcggcgcc acttcgcgaa cgatccgtcg ctcacgcctg
     1201 cgcaaggacg cctacgctgg atgctgcccg tgatgtatcc gtcgtacgcc gcggccggga
     1261 ccgtcttcgt cgagatccat ggagagtgga ggtcgctcgc cggtctcgac gaggtgatgc
     1321 tcgcgagagt gcgcacgctc gacgcgacat gcgcggaccg cctctccctc gcgggcccag
     1381 tcgggaactg cacgaacgcc ggttgggtca tcgccgatgc cgcgctccgc cacgacaccg
     1441 cgcgatttgc gcgagcttgc cagctggcag catctgcgtg caccgggcgg tgataggctc
     1501 gcgcgcatga actggaagac gatcctcgtg ccgcacgact tctcgtcgtc ggccaatcat
     1561 gccgcggcga tcgctcgcga cgaagcaaag gcgcatggat cgaagttgct gctgctccac
     1621 gtgatccagc tgcccggcca gttgcagccc gacgtcgcga tcattcccga gccgaccggc
     1681 gcgccgatca gcatgcgtga gtacgcggtc aagcaagccg aagcgcatct cgaagatctc
     1741 gccgcacgac tcgcaaagga cggcgtcaac gcgcagacat tcctgcgcat cggcaacccc
     1801 gtcgacgaaa tcctcacgtt cgtcgtcgag aaccacgtcg acctcatcgt gatgggcacg
     1861 catggtcgga gcggcgtcgc gaagatgctc gccggtagcg tcaccgaacg ggtggtccgc
     1921 gcgagcaccg tgcccgtgct cacgacgcgg catcccgagt agtgcgcgcc accctcgcac
     1981 tgctgctcgc cgcatgcggc tcgtcgaaat cggtgccgca aacgacgccg acaaccacca
     2041 cgacgccgac aatctcgacg acgcccggct ccaccggccc tgccgtctat ccgccgcccg
     2101 atctgtctcc gcccacaacg ggcctcgcgg tgggtatcgc cgcgcacgtc gtgatcgatg
     2161 atcgtggctc gcggctgttc gtccgcaagg cgaatggcat cgacatcctc gcgctcgact
     2221 ccggcaagaa gacgggacgc atcgacatcg gctgggacgg cgagctgtgg cccgcaggtg
     2281 cgcacgtcct cgcactgcgg atcgccccca gcgcagtgct cgaagtggcg ctcgtcgatc
     2341 cggcgacgaa caagatcgcc gcaacatgca gcggcgtggt cgccgtgccc gcgaattcgc
     2401 agatctcgcg cgtcgacgaa ttcacgacgc atgcaggcac gacctatctg cagtgggaga
     2461 cgtcaccacc gccgcgacga atgggcggtg cggcggtcag cgatgagcag atggctgcga
     2521 tcaccagcga gtttcgagcg gcgtacgcgt gcggcctgtt cgcgattcac acgaacgact
     2581 cgcgctgcac gctcgacaag gcgacgccgc aagaggccgg cctcgaagcg tgcggatcgc
     2641 gatcgatgcc gtggaaccgc tacctgccgt cgccgatcgg caagctcgcg cttcgcgtcg
     2701 atcgctcgtc agcgggaaaa gcgggccttc tcgtcgatac cgatacgctc gtcatcagcg
     2761 agctcggcgg cagcgagcta tggaagctgc ccatcgagac acgcgcgatc gagccgcctc
     2821 cgccttagtg accgtcgcgc cagtaacggt ggttgagcga cgccatcgcg cggcggagca
     2881 gcacccgcgt ctcgtcgttg ctatcgagtg cgagcgacgt cgtcagatcg tctagcgtct
     2941 cgcccttgat gtagcgcgtg aggatgtcgc tgtaaccgtc gcgtgccgtg cacgtctgac
     3001 gcgacttgag acgccaatcc gccgtcgacg atccgaacgt cgtgtcgtag atgcgatgca
     3061 ttgcgtcctg gcgcggatcg gacggaatca cgatctcgat cgcttcgcga tcggttggcg
     3121 ttgtcggcca cgcgacgaag tgctcgccgg tcgaatcgcg ccagctcagc cgcccgacga
     3181 ggttgccgtc ggaggccttc acttcgctac cgtcgcgcat cagggagtaa ccctcgcaca
     3241 cgtactcggt cggtttcgtc ggagctttcg gacgtggcgt gcaggcggcg gtcacgagag
     3301 cgagaaggat gagcttgcgc atggcgaacg tcacgagcac gaagtggacc agcgaaaacc
     3361 tcgccgtatc ggcgtcgaga ccggcagtgc ttgccgacgc aggcaaaact tgccgcgcaa
     3421 gaactacagc ttgtactgct tgagcttgag cgtgaacgtc cggatcggca tgtcgataag
     3481 ctgagcggcg cgcgtcttga cgccaccggc cgcagcgaga gcctccgcca tccggcggcg
     3541 ttcgagatcg cgaagctcgt cggagatcgg gcgaaacgtg acgttcccgt cggaggcggg
     3601 cacaccgtcg ggtggcgtcg ccgctgcgcc tacactcgcg gttcctccga gccgctcggg
     3661 cagatcgtac ggctcgatgg tctcgtcggg cgcggcagcc atcgcgtact ccatcgtgtg
     3721 cttgagctcg cgaacgttgc ccggccacgc gtacgtcagc agcgtctgca tcgcctcggg
     3781 tgacaccgtc atgcgcggcc ttcccgagcg cgcctgcacc tcgtcgagga acgcgcgcgc
     3841 gagcaacggg atctcgcagc ggcggtcgcg caacggaggc acgatcacgg tcgcgccgga
     3901 gagccggaag aacaagtcct gacgaaaccg ccccgccgcg acttctgctt cgagtgagcg
     3961 gttcgtggcg ctgacaacgc gcacgtcgat cgctcgctcg cggacttcgc ccaagcgggt
     4021 gatgcgcttg ctctcgagtg cgcgcaaaag cttcgcctga agtgcgaggg gcagctcgcc
     4081 gacctcgtcg agaaacaccg tacccgccga agcgttctcg aacagacctg cctttgcagc
     4141 ggtcgcaccc gtgaacgctc ccttctcgta gccgaacagc tccgcgtcga cgagcgtctc
     4201 gggcccgagc gccgcgcaat tgacggcgat aaacggtccc gagcgtttgc tccagtgatg
     4261 gaccgcgtac gcagcattct ctttgccgac gccggtctcg ccgctgatca ggaccgcaag
     4321 ctcggttgcg gcgacacgct cgagcagcgc gaacacgcga gacatcgccg gatctccgac
     4381 gagcacgcgc cgtgtaccga gctcgatcca ctgaccggtc tccgctgccg cagccacggt
     4441 gcccggcttc gcccgacgca gcgcggagcg ggccgcgagc acgatcgtat cggcatcgca
     4501 ggcgtccgtc gggcattcgg ccatgcctgc acgcgcacca ggaatgcccg ccagcgctcg
     4561 ctcgatgact gcgtgtgcat cggctctcgc gatttctggg agtaggacga gcacgtgccc
     4621 atcgtcggct gtgccgagca cgtcgatgag gcgtagcgcg ttgccaagct ccgcgacctg
     4681 ggcggccgca cccgcgatca cggcgacgac aagcgagcgc ttaaaagtga tggcgcgaac
     4741 gagctcctcg gcaagccgac gcttccagcc cggttcggca tacgcggtcc gcgcgacgag
     4801 cgatggggcc gagaagtgaa cgacgagcac gacatcgccg acggtgagca cgtcgcccga
     4861 cgccaacacg cggctctcgg tcaccggctc gccgttcacc cgcgtcccgt tatggctccc
     4921 gaggtcggcc gcacggacga cgccatcgtc gacgcgaacg gttgcgtgac gtcgcgagac
     4981 cgacgcgtgc tgcacgcgaa gctcgacgtc tttgtcgcgg ccgatcacga tcgcgccgct
     5041 tctcggaagg tgaaagatcg acgacgtatc gttatcgacg acgagcaagt agaccgtccc
     5101 cggaccctcg ccgtcgtcgc caaagcgcgc gagccggctc gccgtctcct cgacgttgct
     5161 gctcgtcatc gcgtgttccc ctcggcacgc accacggtat cggcatccag gtactcggga
     5221 acgtgcttgg ctttcatcgc ggcagccagc tcggcagcgg agcgcgtatc gacgtggatg
     5281 tcccaggcgt cgagcgtgcc gtcctcgctc gcgacccaca gcgtatcgcc atcaatgcgt
     5341 accgagctca ccggtcgttc gtgataatcg cgcgagccaa ggcgctttcc tcgctcggca
     5401 tcccagacgt acgcgcggcc gtcatcgccc gccgtcacga tcatcgacga gtcgtcggtc
     5461 acggcggcgg ctcgaacgat tcccgtggct ccgtcgagcg cgagctcgaa cgacagtttc
     5521 ccgtcgtgga cgcgccacac atcggcgagg ccggggcctg cggcgacgag gatggttcca
     5581 ttcttcgaga acgcgagcgc gctatacggc cgcgcagcat cgctgatctc gacaagcttc
     5641 tcgccactcg cactccactg cacgagcgca ccgctcgctc cgacggcagc gaacgagcca
     5701 tcgcgaggat tgatcgcgac ggcgtcgagc cgctttgcgt tcgacggcag cacgagcttg
     5761 cgactcttgc tcgcgatcga ccacagctcg acgtgatcgc ccgcgagcgc gacgaccgtg
     5821 tcctcgtcac ccgagattgc gagctcgcgc acaggcgttg gaatcgcgag ccgcgcgacc
     5881 ggcacgcgct tgtcggtgtc ccacacgatc gattcggcgt cctcgccgcc cgataccaac
     5941 agcgccttcg ccggcgcgaa cgcaaccgca aacacgcgtc cgagatgacc agcgagcggc
     6001 gccgtgtctc cagctcgcac gagcgtgacg cgactgtcgt cggtagcgac cgcgacggta
     6061 ccgtcatgcg cgatggcgat tgcatgaatg accgagtcga gctcgtgacg gatcttgatg
     6121 gtcgccgtgt tccagagacg cacggttccg tcgcggctcg cggtggcgag cgtcgtgccg
     6181 ccgacgaagc gaacactcgt gacaacgtcg gcgtgctcga acgtcgcgat ctgctgcccc
     6241 gtctgcaggt cccagatctt cgcgagcgcg tcatagccgc ccgttgccat gaagcgaccg
     6301 tccgcggata ccgctgccgc gacgacagat ccagtgtgcg cgacaaaccg gcggacgacc
     6361 cgtgccgcgg gtggtgcttc gccgaccggt agctctgcgg gcaacgacca gatatagacg
     6421 acgccgtcgg ctccggccgt caccgcacgc tgctcgtcga ggaggagcgc gacgttcacg
     6481 cctgcgccct gcatgtgcgc gagcggcgcg acgctcggtt tcgcaatggg caccgtcctc
     6541 tccgtgtcga cgttccagac gcgcgcggtc gcgtcatcgc tcgccgtgat cacggacttg
     6601 ccgtcgctgc tccacgttgc tgcattgatc cgaccgcgat gcccacgcag ctgcttctgc
     6661 acggatccgt cgacgacgct taacacgcag gccgtcgcgt cggcgctcgc gctgaggact
     6721 tcgcttccgg atgccgaaag ctccagcgag acgaccggcg cgctgtgaca cgtgtccgac
     6781 gcgctgacgt acttggcttt cgttgcgagg tcccagacga tcacggatcc gtcttctcct
     6841 ccggtaacga ggcgcttgcc atcccggctg aagaccgctg cgttgatcgg gcccgtgccg
     6901 ccctgcagat ccgcgacgag ggagccatcg cgtgcccaca cgtgagcgac gccatcatcg
     6961 ccggctgcga caacgcgcgc gcccgtctga tcgatggcga cgacacgact cgtgccgcga
     7021 ccaccgaagc tgcgaatctg cttgccatcc gcagaccaca gcacgacgtc gccagcgccg
     7081 gacgtcacga gctcggtccc gtcgtcgcga acggcgagtg tgacgttgcc ctttcccact
     7141 tcgatgtgcg cgatctcggc gtcgaacggt cgcatcgcgt cggcaatcaa gaagccgcgc
     7201 gcgccgccgc gatttccatc ctcggacgct cccaccaaat acgcgagcgc agcaccggag
     7261 cgtccggcga gcagctcgga gcgcccgtgc tcttggagaa gctcgatgcg acccgcttgc
     7321 gccttggcct gcgccgcctc ggcccggtgt ttctcggtga cgattcgcga cacgctgatc
     7381 acgccgagca ccaccatcgc gacgagcgca gcggcgccga cgccgaccgc gaggcgatag
     7441 cgacgaagcc aacgcacgac gagctggctc accgtgtagc gatgcgcacc aacgagctgc
     7501 ccggtctcga accgcttgag gtcctgcgcg agcgcactcg ccgtgatgta gcgatcatcg
     7561 ggatcgcgct gcatcgcttg gttcacgatc gcgacgagct ccggcggcgc atccggcacg
     7621 acctcgtgca ccggtcgcgg tggtccagct ttgacgagct cgagcacgtc tcggctgtgc
     7681 tctccggtgt acggcggcac gccggcgagc aacttgtaca gcagggcgcc gagcgcgtac
     7741 acgtctgcgc gttgatcgac agcttcgccg cgcgcctgtt cgggtggcat gtacgcgggc
     7801 gttccgacga tgctccccga cgctgtctct tccgcggtcg cacgcaatcg caacgccagc
     7861 gactctttcg gatcgctcgg cgcgccgaga tccttcgcga gcccccaatc gatcacgacg
     7921 gtctcaccgt actcgccgac gaggacgttc gcgggcttca ggtcgcgatg gatgacgttt
     7981 tcgttgtgcg cgtatgcgag cgcgtcggcg accgcgatga cgtgcggtag cagcgcgagg
     8041 cgttcggcga gtgtctgctt ctcgccgacc actttgtcga gcgagcgacc cgacacccga
     8101 ggcatcgcgt agaacggttc tccgcccggc cacacgcccg cttcgtagac gtgaatgatc
     8161 gatgggtgct gcaagcgagc ggtgattcgc gcttcgcgtt cgaatcggcg cgctgcatcg
     8221 cggttcttcg gcagcagctc cttgatcgcg acgtcgcgcc cgaggcgaag gtcacgcgct
     8281 tcgaggacgc ggcccatgcc gccggtcgcg agctccccgg tcacgagata gtggcgacgc
     8341 tccactgtcg tcagctgcgc gtaatctcgc gcaggaacga ccacatcgat cgcagcgaca
     8401 ggcgcttcac gcgcacgatc gttggcgtcg atcgtgtctt ccggatcggc ggctcgcacc
     8461 tcgccgtctc gcactgcatc ccccatgccg cgtccaaccc acgatggcat gaccgctgca
     8521 aaagcggcaa gcatgatacg gtggtcgtgg gtgatgcgac gacgaacgtt gtcgttaggg
     8581 gtgctcgcgt ccgtggtaac cgggtgctgg tcggatgact tgcgcagtga cgggttcacg
     8641 gacgcgcaat ggactgcact gcagaagcag tacacgctcg agtcgccgcc ggagatgtgc
     8701 tccggcccgt gcagcctcgg ctttccgctc ggccaacgcc tgttcttcga gccagcgctg
     8761 tccgggaacg gtgaagtgtc gtgcgcgaca tgtcacgcgc cgaccggctg gtacgtcgac
     8821 cctcgccgga cggacgtgtc gtttggcacg gtgaagtgga cgcagcacaa cacgatctcg
     8881 ctcgtgaacc tcgcctacaa gcgcacgttc acgtgggccg gacagtgcgg cgatcattca
     8941 tgtctgtctc ccaacgacgt catcgtcgac atcgcgctcc ccaaggcgat gacaagcacg
     9001 cgcacgctcg tgacgcagac gatcgaaaac aatcccgagt acctgtatct ctatcgttcg
     9061 tcgttctcgc tcggggatcc ggacgtcgtc gcgaatgtcg ccacatcgct ccacgcgtac
     9121 atgcgcgcgc tcgtcagcgt cgacgcgccg ttcgatgcgt acatcgccgg taacgacagc
     9181 gcgctctcgg acaaggcaag gcgcggattt gcgctcttcg tcggcaaggc gatgtgtgcc
     9241 gagtgccacc gcggtccgat gttcacggac aacacgattc atgtcaccgg cgtcgcgcaa
     9301 cgcggcgagc acgcccctgc gatggacgcg ggtgcgcccg gtttcttcac gcccggtctg
     9361 cgcaacatcg acaagaccgc cccgtacatg cacgacgggt cgctcgctaa cctcgcggat
     9421 gtgatcgact tctaccggcg cggcggagat acgagcggtt tctcaggcga caaagatccg
     9481 ctgatgcagc cggtcgacat caccgacgac gaagctcgcg acctcgaggc gttcttgcac
     9541 gcgctcaccg gtcaagcgct tcctgagact ctgacgatgg acctgcgctc gtacccacca
     9601 cctccgacgt gccagctgac gacatgcggc accgcgtgcg ttgacacgaa cgacgacgcg
     9661 atgaactgcg gtggctgcgg gaaggtatgc ccggtgaaca agccggtgtg cgcgggaggc
     9721 gtatgcgggc actagcgatc gttgcgctgc tcgcctggcc gctcgtcgcg tctgccgaca
     9781 cggaaccggc gcacgacgag gctgaggacg acgaagcgcc cgttatcacc gacgacgatg
     9841 gcgacgagcc gacgccggca gagctcgccg acgaagtcga cgatctggcg cagcgccaaa
     9901 agaagctcga gcgagcgcag cggacgaacg acgagacgcg ccgcgagctc aatcgcatga
     9961 agtacctgat gcgattcatc aacgtctacg tcgacgtcgg agcgttcgcg gtcgccggtg
    10021 acggatcggg gatccgcagc gacgtcggtc atgtctacta ccccgagtac atgggcaagg
    10081 tgcctggcca atgggtcttc atgggcgatc cgttgtcgac ggcgatcaac tcgcagggcg
    10141 agccgagcga tacgtcgagc tcgcgcgaaa ttgccgtcga caccgtgaac agcgagggtc
    10201 acccgtcggt gatcgtcaac gcggtcgggc tgtcgatcgg caaagatctc acgcacgaca
    10261 tcgccgttgc gtccctcacg cagctcatgc cgcgcgtaac gagcaacgtg ctcgacgttc
    10321 agctcgccga gatcttgtat cggcatcccg tcggcgacgg aaacctgcgc gtgagtgccg
    10381 gcaagatcga tagcgtgctc ggtatcgagt atcgcgcgca ggatgcgccg gtgcggctcg
    10441 gcgtctcgcc gtctctcatt tgccggtaca cgtgcggccg gcctctcggt gttcgcgcac
    10501 agctcgtcga gggagccatt tcggtgtccg cgacgatgac gaacggcaac aacttccagg
    10561 cgctgttcga gcacgactcc aagctcaagg cgaatgctgc gcccaccgcg agtgcgcacg
    10621 tccaatatct tctgcccgtc ggccaaggcc tcgaggtcgg cgtctcgggc gcgctcggtc
    10681 ctcaggacaa tcagccccat atcgacgtca cgcaatggca catcggcttc gacgcgaagc
    10741 tgacggactt gcatggcttc gatgctgctg ccgagtacgt tcagggcgaa caagagggcc
    10801 aaacgacggg catggccgag tgcgatgccg ccccgtgctt gacgtacaaa ggtgcgtact
    10861 tgctcgtcgc gcgtcgcgtc caagcgtggg tgacacccta cgcgcgcatc gactggcgag
    10921 atgccgtgca taccgacggc gctcgcttcg tctacgagtc acacacggtg cgtggcaccg
    10981 tcggtgctca tttcgaaatg acgagccgca tcctcgcaaa ggtcgagtac acgtggaatc
    11041 acgagctcgg gaacatcccg cagttcgcca atgacatcgt gacgagctcg ctcgtggtgg
    11101 cgaccgactg atgcgtcgcg cgtacgttcc tctcgtcgcg ctcttgccgc tgctcctcgg
    11161 cgcggagggc gcaacaccgc cggcgggcgg ctcgatcgcg ggcagcgtcg tcaccgtcaa
    11221 ggatggcaag ccggcgaagc gcgacgacgt gtacgtgtac ctccagcgcg tcggccgttc
    11281 gaagaatcgc gagctccccg gtaagggcgt gcgcaaagag attcaccaga agggcaaagc
    11341 gttcgagcct cacgtcgtcg tgattcccgt cggaggcgtc gtcgcatttc cgaacgacga
    11401 caaagaagac cacaacgtgt tctcgccgac ggatcccggc ttcgacctcg gccgctacac
    11461 gaccgacaag aagggcaagc cccacacgtt cgaggacgcc gacgagttcg acatcttctg
    11521 cgacatccat ccgcagatgt gggcgaaggt gaaggtcgtc gagagcgagt acatcgcgca
    11581 ggtcgtcgac ggaaagttca gcttcgaagg cgtcgcgccg ggcacctaca aggtcatcgc
    11641 gtggaccagc gatagcgccg aggtgaagtc cgacaaggtc gtggtgacgg cgggccgaag
    11701 catcacgctg ccgaacgcgc tccacctgca gctcaccgtg gcgtcgggct gccacgatcg
    11761 caaagacggc acgccctacg acgcaaagta cggtcgcagc tcgcgctgcc cgcagaaata
    11821 gccagcaagt tttgccgacc ccggcaagaa ttgcctggtt tgcgtacccg gcgaagggtg
    11881 agagcgtggc gccgacattg ctacgtgcgc ccgcatgcga aacacgatct ggtgtgtggt
    11941 gctcgtggcg gcgtgcggcg attcgaccag cgtggcgatc gatgcgcgtc ccgattcccc
    12001 cgatgtcgat gcggctgacc cgttggcgct cacgaccgag gagctcgcgg ctgcgcacac
    12061 gctgtcgccg ctgcccgcgg taccagccga cacgacgaac gcgtatgccg acaacgcggc
    12121 cgctgcgcga cttggccaaa tgctgttctt cgacaagagc tatgcaggtc cgctcgtcgt
    12181 cggcgataca ggcacgaacg gcgggctcgg caagccgggc gaggccggca aggtctcgtg
    12241 cgcgtcgtgc cacgcggccg gcagcgaagc gatcgatgat cgccggtcga ttccgaacgc
    12301 cacgtcgctc ggcacggatc gcggtacacg caacgcactc ggcgtcgtca actcgtcgtt
    12361 ctacaagtgg acgaattggg gtggccgctt cgactcgcag tggtcgctgc ccatggccgt
    12421 tgccgagaac gcgaagatca tggacagcac gcgcctcgcg atcgcgcaca tgatctatgc
    12481 gaagtaccgc acggagtaca acgcgatctt cgagcccgat ctcgatccgg atctcgatcc
    12541 ggccgcgtcg aacgcagcgc ggtttcccgc gaccggcaaa ccgggccaga ccaacttcga
    12601 caacatggcc gcaggtgaca agacgatcat caatcggatc ttcgccaact acggcaaagc
    12661 actcgcggcg tacgtccgca cgctcgtgag ccgagacgcg ccgttcgatc gcttcgtggc
    12721 gggcgatcat tcggcgctga cggctccgca ggtacgcggc ctgaagctct tcatctcgaa
    12781 gaagtgcatc ggctgtcaca gtggacccaa tttcagcgat gacgagttcc acgcgctcgc
    12841 gtcggcaccg gtcaccgaca tgggacggtt cgcagatctt cccgtactgc tcgcgagcac
    12901 cgcgaacgtc aacagcgcgt tgagcgacga caccaacacg cacaagctgg acgggctcgc
    12961 ggcgatcgat tcgatgaagg gtcagttccg cacgaagagc ctgcgcaacg tcgccggctc
    13021 cggaccgttc atgcatggcg gcacgctcgc gacgctcggc gacgtcgtcg acttctactc
    13081 tcagggcggc ggggatgtcg gggcgagcgg tgtcaccaag gacacgcgga tgacgccgtt
    13141 cgccctgacg gcgggcgaga aggctgacct cgtggcgttc atggagtcgt tgtcgggcgc
    13201 gcccgttccg gcaagcgcga tcgtcgatat ttcgaagtga gtcgccgtgc gccgatgacg
    13261 cagtttcgag atcggcgtga cgatcacgcg cgttgatcga tcgcgatcga atcactacgc
    13321 tccggtgccg tggaacgtcc acgcactcgc tcgcgacgca acttgccgcc gattcgcggc
    13381 ggagagatca tgcattcgtc gccaacgaat cccggggcgc ttgcatccgc tgcgtggtgc
    13441 gccgagcgcg cgctgttccc gaaagatccg aactggtccg tcgagatcgc gctgcacgcg
    13501 gacgagtggc gcttcacgat cgagatctac gcgcaagagt ggggcttcgc cgtccgtcat
    13561 ctggagcgga cgtcgtggat tcgcgtgacc gacgtcgcgt tcgtccacgg gagcgacgac
    13621 ttccagctcc tccgcaatac gccgcacctc ggttcgatcg gggagcttat cgcgtcgatc
    13681 gagatcgatc agcggatcgt ttttggtcgc gcgaacgcga tcgttcagtc gtcgttcggc
    13741 ggcgaggatg tcatccgcgc ctgggttcgt tcgctaggcc ggtgacgcaa cccgcgccgc
    13801 tggcgcgaag acgagctcgc gaatcgcctg cttgagctcg cgactcgtga gctttgggcc
    13861 gaacgttcgc tcggcctgca tgttcgacga gcccaccgcg atcaccggag tggatttgcg
    13921 gcggcttgcg acgcgatcga ggtcgctggc tgagaggcca tcgctcccga cgatgaggag
    13981 gtccagctgc gtgggtacat ctgcggggtt cgcgatggag ctgaggtcga gctcgccgat
    14041 ccacgtcatg agccgaagct cctcgacgat ccaggcgcgc aagtcctcat ctgccccgca
    14101 cacgcaaaca cgaatgcgat cacctgccac agatccatcg agccaaacct tggcctccga
    14161 tgcacgccaa tcagcggttg ctcacgttag aggcgacctg acaatcgccg tcgcgccgaa
    14221 taccccagcg ttacccgcgc gcacgtcacg ctgagcgcgg ccgctcgtgc actcgcgagt
    14281 agccgctcat tcttatcaat aggctttgtc gttcacggaa cttgcaagcg gggttacgcc
    14341 gcaaccgatg ctggaaacga cccgcgacga agacctgaac ctgcgcgtcc gcaagaacgc
    14401 ggtgctccgc gcatgggcgc gtcaaacgcc gcgcgggagc ttgatggtcg ggcctgtcga
    14461 cgtcgtgctc gacgagctcg tcaacttcgt cgtcggcgag gatggatccg cggagtcgtt
    14521 gtcggcggcc gtcgaaggtc acgttgctgg ccggttccgc gatggtttca cggtcgacgc
    14581 ggtgattggc gagtacgccc tcctccgccg ctgcatccat cgcgagctcg gcaatcgccg
    14641 attcgatcat cccgtcgatg tcgcgattgg ttatgcagcc aagcactacc tgacgatgcg
    14701 tgaagagcta cgcgagcgat tcatcggtgt gctcgcgcat gacctccgca gccctctcgc
    14761 gtgcgtgacg atgggcaccg agatgctcgg cgtagacgag cggagcgagc acgaacggtc
    14821 gctgttgaag ctcgtgctcg agtcatcgga tcgcatgcgc cgcatggtcg atgagatcct
    14881 cggctgggca aagcgctctc ccgtagaagg cttcccggct gcgcagcggt tcgatgacat
    14941 ggcggagatc ctccaggcgg tcgcgcagga gacccagctc gttcacggcg tcgatgcgat
    15001 cgagctcgag acgaccggcg atctacgggc ggagctggat cgcgaccgcg tgcatcaagc
    15061 cgtcgtgaat ctcgtgcgca acgcgatcga gcatggcacg ggctcggcgc acgtcagcgc
    15121 gagcgaagcc gatggcggtc agacgatcgt gctcgtggtt cgcaaccgcg gcgcgctgcg
    15181 gtcgccgaat gcgtcggaca tcagccagcc attcgagcgc cgcaagcgtg caacgtcttc
    15241 gcgtgggctt gggctctaca tcgtcgacca aatagctcgg tcgcacggcg ccgggctcga
    15301 catgacgtcg aacctcgacg agacggcggt cacgattcgc tggccgacgc gccgcatcgt
    15361 cgacggcccc tcgttctgag tggggcaacg ttgcggcgcg cgcaagcgca cgctgcgcac
    15421 ggtccgctcg aagctccgga accgccgcac gccttgacgc gtacttaggt tttcccttgg
    15481 tgggcccctc gattgcattc cagcgactta cctccgtgcg cctcgtcgac cgactcaacc
    15541 aggagacctc gagcctccat gccgaggtcg acgaagagct cgtcaaacag ccggtgagcg
    15601 cccgcgggta ccagcggttt ctcgctcgca cctacggctt cgtggcgccg ctcgagcggg
    15661 cgatcacgac gacgccagcg atcgaaaagt atgtcgatct gcggcgcttc aataaggaag
    15721 agctgctccg ccgcgacctg ctcgcgctgc actacacgaa ccatcagatc gacgggctac
    15781 cgcaatgctc ggtgccgctg ttcaacacgg cggccgaggc acttggttgg ggctacttca
    15841 tcgagcgcag cacgctcgcg catggcagcg cgtttcgcgc cgtagcgtcg aacattcccg
    15901 gcgaggtcgc gttcgcatcc tcgtatctga agtgctactt cggcgcggtc ggcgagatgt
    15961 ggcgcgcgtt cggcaatacg ctcgatcaga tcgcggcgga caccgacacc gacctcgtca
    16021 tcgaagccgc gcgcaccgca ttcaagttct atcagcgttg gcgcgagccc gatcgcgaga
    16081 gcacgcagcc tgatgtcacg tcgacctcgg gctcgctgcg gctcaccgcg atcgagcagc
    16141 tagcgcaaga cgacgacagc accttgtcct agccactctc tagtcgtttc ggcgcaacac
    16201 gtcggcgatg ccgtacacgc ggcggccacg ccgttcgcga attgtcacgt agatcgccgt
    16261 ggagaacatg ctcaagcacg cgaccgacat cgcccagtag cggtgcgctg gcgcaagcac
    16321 ggtgacgagc atcgtgccga cgaacacgac gacgggcgcg agcatcgcgc gatcgagaaa
    16381 cacggagcca acggcggatg cgccggtcca catcgcgagc gcgagcagat gcgtctgcca
    16441 cagctcgaga ccgccggccc atgcgatggc ctcgacggcg gcatgcagca cgacgccgat
    16501 gaacaccgtc atgagcacgc cgcgattgag cgcggtcttc gtcatcgagt cgcgcgccca
    16561 ccagccaaac acgacgattc cgacgagcat cgtgataggc gcgccgaacg actgtgcgtg
    16621 gctcacgtga tcgatcacgt taccggcgag tgggatcgcc gaccacaaga tgccgagcaa
    16681 cgtcaggaag aacgagcgcg tgagctgccc cgcttggacg tcttggtcga gctcgaaccg
    16741 ctcgaggcgc tttcgcttct cggtggcgtc acgctcgacg gtggccagcc ggtctgcgag
    16801 cgctggacgc ggcgtcgaga gctcggcgat gagcactgcg gcggcgcgcg cgtcgccgtg
    16861 gtcgagctcg tgcgtcgcca tcgcagcgag ggcgcgatcg aggccctcac gtgcctcggg
    16921 attctcgggc cacgcgcgga gcgcggcgag aaagccgaag cggcactgcc cgaagagctg
    16981 atacatcgtc acgcgcgtcg catctttcgc cgcgagctgg gtctcgagct cgcggagctt
    17041 ctcgtgcgat tcgtgcgcga tctgggcgct cgttcgacgc tcgagaaacg cggcaactgc
    17101 gagccgtagc tcgtccgcgg tctgatatcg ctgcgccgcc tcgcgagcca tcgcccgtcg
    17161 acaaatcgcg acgagctctg ccggaacatc cggcgacagc gacggctcgc agagagcgat
    17221 gctcgtgatc gatgcgccga tcgattcgag caagtgcggc ggcctgccct cgatgatctc
    17281 gtacaagatc gcgccgagca gaaacacatc cgtctgctcg gtcaccgcgg cgccgcctag
    17341 catctccggc gccatgtacg cgagcgtgcc ggcgacctcg cgcgactccg ctgcgagcgg
    17401 cagccgccca ctaccatcgt ctttcgtcgc gacagcgatg ccccaatcga ggacgtacac
    17461 ctcgccgaag cgtccgatca tgatgttgtc gggcttcaga tcgcgatgga cgatgccgcg
    17521 gctgtgcgca aacgcgatcg cgtgacacac ctgcatgagc gtcgcgaggt tgtcctcgag
    17581 gtcacggctc ttcgggtgcc acggctcgcc ctcgatgcgc ttgagcacga gcaacggccg
    17641 cccgcgctcg tcgctgccga ggtcgtgcac cggcacgatg ttcggatgct cgagcgcacc
    17701 ggtaatccac gcctcgcgca gaaggcgcgg tgcagcctcg tccgtcgcgt cgctgcgcag
    17761 cgtcttgacc gcgacctcg
//
LOCUS       DATLBZ010000589        17821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7155491, whole genome shotgun sequence.
ACCESSION   DATLBZ010000589 DATLBZ010000000
VERSION     DATLBZ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17821
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7155491"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VMZ53_29070"
     CDS             <1..667
                     /locus_tag="VMZ53_29070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32602.1"
                     /translation="AIAEPAPERPGLGTTFGEQVYAPISFAPFTRASSAPWAEAALHY
                     NDADGVEAHARYLGTRPLPLELYAGDGSISVALTDDYGHTLPGLTAQGRTMIVGEDGQ
                     RYKIVVRNATTARFEIVASVDGVDVIDGKPADPNRRGYIIDPHDTLVIDGFRTSDANV
                     AAFRFGRVADSYAAKSSGDRNVGVVGLAIFSERGAVWTRGELQRRDSANPFPSRGYAM
                     PPR"
     gene            688..2331
                     /locus_tag="VMZ53_29075"
     CDS             688..2331
                     /locus_tag="VMZ53_29075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32603.1"
                     /translation="MPYNDVFHPGSTLGKYEIVRELATGGMAQIYLAKVTGTAGFEKN
                     VVLKCILPNLANDRQFVTMFLDEARLAATLRHSNIADVFDVGVEGKTYYFAMEYVAGQ
                     NARTVRLKAREAKRPIPIEISLAIVGGTAAALHYAHTRTDANGKSLDLVHRDVSPSNI
                     LVGYEGAIKLVDFGIARATSRRLRTRTGIRKGKVPYLSPELCRGHHIDHRSDLFSLGT
                     ILYELTTGERPFLGNSDFVVMDQIVTGTPLPPSSTTKGYLAGLEPIVMRLLARSPEAR
                     YQTAGQVLEDIETLASENRLLISSHIVAKFMKELFIDAGTAPFERDTYLDFAQPVVDD
                     EKHADEEPTAAFSRAEQMWTAELKVKVRTPRRAQSEPGEEISITSAVFHEPTRQDVVP
                     AFDPIDARSEEILIQLDAEAPDDESRDRRAHRRIITLIDRALEWVDSGELDKAVTAVE
                     LALDEDMSVPGTQDLLQDRVASIVAVYEAMLEDPYRVLELARPIDELAGVAMEIGART
                     LLAWIDGKTNIQQLLAHAEMPRLEAYHHLCQLLMRGMIR"
     gene            2374..3090
                     /locus_tag="VMZ53_29080"
     CDS             2374..3090
                     /locus_tag="VMZ53_29080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS32604.1"
                     /translation="MPTYDDELLAPIDTLRGYDRWAPTYDHDPNSVVAATSWVLDRAP
                     LGVADADVLELGCGTGRNIPRILGEGARSYLGLDGSHGMLTIAAQRYQDPRVTFGVTD
                     LTAQWALPKQFDFALIVLVLEHLPILDPLLVSLARAIRPGGRVRIVDLHPERIASGSF
                     AHFQDGTTSVHFSSVAHAVPMLCAGLEAVGFESVRRDWLAGETMLAEVPGISKHRGTR
                     LVLDLKATRRSRDRRGSGSL"
     gene            complement(3087..3356)
                     /locus_tag="VMZ53_29085"
     CDS             complement(3087..3356)
                     /locus_tag="VMZ53_29085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32605.1"
                     /translation="MPRGDKTKYTDKQKRKAEHIAEGYKERGVSAREAKARAWATVNA
                     IHGGGEKSGSGRGKPENKAPYRKGGRVGGAASRGKTRAASRSRSR"
     gene            3544..3885
                     /locus_tag="VMZ53_29090"
     CDS             3544..3885
                     /locus_tag="VMZ53_29090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32606.1"
                     /translation="MPDLDTSRIPREALIGLLSESSNIDRERVTAPMPVARLDELLGE
                     DKIEEVLNSDNPGAVVEEAEPLATGSNAELIVRFKQQQQPVTSTVMSHHVIVWGCAVA
                     LLIVALILVLV"
     gene            3889..4101
                     /locus_tag="VMZ53_29095"
     CDS             3889..4101
                     /locus_tag="VMZ53_29095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32607.1"
                     /translation="MDTTADTAIRDTLEMPPTQLESILVHERTTAEDVVIELTKRKRK
                     PLQTIHLVAISFISGIVLGLAAVAVV"
     gene            complement(4098..4556)
                     /locus_tag="VMZ53_29100"
     CDS             complement(4098..4556)
                     /locus_tag="VMZ53_29100"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HUS32608.1"
                     /translation="MTADEIVRAAGIYAGTFAVGAISSVIPLISIEVFLVAITLAHGT
                     ANAVPLIVLATVGQVLGKLPVYATTRGLAALPGPQRRWIERVRVWVARFGNRPNLVLG
                     TSALLGLPPFSIISTAAGALAIPPRTFCAVIAVGRALRFTAIVAVAALYG"
     gene            complement(4556..5479)
                     /locus_tag="VMZ53_29105"
     CDS             complement(4556..5479)
                     /locus_tag="VMZ53_29105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUS32609.1"
                     /translation="MSVVVETWNTKGDVPTLARQLDRLAPQISALDAELVVTYVDLSA
                     GTRETLEQRHGRRIHWVQLDGRAGYYDHKNAGFDATTGNVVAFIDGDCEPSEGWLAAL
                     VAPFSNHAQVVAGATSYDGVLAPLANELDFPYFEYADKRRSFAATSAKTAPEVRNFFA
                     NNVAFAREAFASRRYPTIEPMFHGQCQVLALRFMEAGIPIAYAPDARVTHAWPDDLRE
                     FLEVRLLRGADTTQLLPHVLGTYAPRTRSTVEKLGPLPALAIFAMRAVTGALSAMRRG
                     PVVRGLALVAAVTAIDAIGAVAAPAVYRWVA"
     gene            complement(5485..6612)
                     /locus_tag="VMZ53_29110"
     CDS             complement(5485..6612)
                     /locus_tag="VMZ53_29110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HUS32610.1"
                     /translation="MSDTVDDINGICVGLRRLVAAGRRAGHEVHLVGAAKPGVVDEVV
                     RIPSAMTASLPIYPDMTWSVPELPPLTSWLARNADIVQVATPGPMGIAGLIAARMLGL
                     PVIAQYHTEVADYAAQMTGLPMVKMIVEPIVGWFYKQADVCLAPSQAVAQRLASFGIA
                     ADRIWRIQRGVDLELFNPARRDRGVLAKWGIGDVPVALYVGRLSKEKNLDALTSAWAK
                     VHAARPDAKLLVVGAEGPLAGTFKAPGMVETGGLFGEELASVFASADVFMFPSETETF
                     GNVVVEAAASGLPSVVARAGASHEHVVAGETGLVVDGKKSQELADAVLELFADPLRRW
                     QMRAAARAHAAGYDLAAAVRATWEIYAKVAAERAQLVEAAS"
     gene            complement(6645..7331)
                     /locus_tag="VMZ53_29115"
     CDS             complement(6645..7331)
                     /locus_tag="VMZ53_29115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUS32611.1"
                     /translation="MTIRELQRRAVDEIKRHALVMSLGSPRAQAHILTEYLWSEEGAE
                     TAALHRTSTSTTPPWVDKLAANQLADEQRHAALLRERLVALGAPTDRQPPSLMRAKLW
                     WVERVTAPYMDAFEAGPVVVLLAIAAQLEGTGVRMFGRHLAVLEQSCADDPTTHTLRE
                     ILSDEKRHAKSCALAVDRLVNDHERTRLEELREKIAKIDRAFGVTISVVFWLMIASYA
                     TRDRLRGGAR"
     gene            complement(7532..7957)
                     /locus_tag="VMZ53_29120"
     CDS             complement(7532..7957)
                     /locus_tag="VMZ53_29120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32612.1"
                     /translation="MGFFSDLKNKVTGGGATVRVSTQGVRRGAAVPVQVQAQAKANGK
                     VNSVYLLVRGMETASWKGENNTTVSNSKVSFETKIQIAGACELKEGQTYDWSGQLELP
                     HNVNATLRGSLINHTWEIQAGLDMAGNDPDSGWQQIDVA"
     gene            complement(8068..8493)
                     /locus_tag="VMZ53_29125"
     CDS             complement(8068..8493)
                     /locus_tag="VMZ53_29125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32613.1"
                     /translation="MGFFKDLKNKITGGAATVRVSVPAGRRGAAIPVQVQAQAKTNGK
                     VSAVYLLVRATENAQFKDSNNEKVSGSKVSYENRVMIAGAQELQEGQTYSWEGQLELP
                     QNAMPSLRGSIIDHTWEVQAGLDMPGNDPDSGWQSFDVQ"
     gene            8573..8647
                     /locus_tag="VMZ53_29130"
     tRNA            8573..8647
                     /locus_tag="VMZ53_29130"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8605..8607,aa:Cys,seq:gca)
     gene            8755..10698
                     /locus_tag="VMZ53_29135"
     CDS             8755..10698
                     /locus_tag="VMZ53_29135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS32614.1"
                     /translation="MVTSVSQQTAPLAPPVGEGVVGDYALVRRIGRGAMGEVWLARHT
                     RTGGIAAIKVLRDGTARNRERLRRFFARERRAVARLSHANIVPLFELGPDYIAMRYID
                     GPTLARRLATPIEPVAALDIAVQIASALAHAHARDVIHRDVKPSNILLDTMGNAYLAD
                     FGLAALLDDADAGDERGGTPQYMAPEQTRGDTPSPAADQFALGCVLAEMLAGGRVQAD
                     PDAVASALPQTLPRRLVDIIRQAIDPDPAKRWPSVGSFAIALRGLDVASSPVPQRLLP
                     ELRVSGPFAWAAHQDAFTELAPSISRADYRLSALEAKGAISPAAARAFRERTGYADFG
                     WSVVGRTDRLGAITDPRAYARAGEAVVMIHGGFATRESWISVATPVAQGNGQTLVFIP
                     DLSGYGVSRYQERRPAQQQVSPQGSLDGVLAWMELIGVRELPLVLVGHSLGATALMTA
                     SDDALGARTWRVAVAPVFPAVDWRQRWGLRLAPAMGLSLAWIPGVKRLVANMTFQLAP
                     AARAYEPEERQRMLEAFMALPAPTLIRSSRQYSYARPAPSTELARCVVVLSENDPVAP
                     MQRMLRALAELEFPEHQIHRLVSQGHLPHGQTAINPEWTQRNIVELAAIIDQLLVAAR
                     EGSVLPTQVASTLMSDGTSTESA"
     gene            10695..11804
                     /locus_tag="VMZ53_29140"
     CDS             10695..11804
                     /locus_tag="VMZ53_29140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS32615.1"
                     /translation="MKRKKDKRTLDDVVDLGRSHVDRGELAQAEACFREALAMEPEHL
                     GVITLLGLLLVEREKFEPAIDLLERARDAAPEFAPVQLALGSAYAGAGHDDLAVVAME
                     TAIKLDTTSTVPLERLAKHYIVARKPREAIGLLRRVLRRDPTHAHAQFLLAGLTGDQP
                     GAAPPSPPAELIADLFDAYATKFDEHLVGGLQYSAPKALAALVSETGAKADGTWRVVD
                     LGCGTGLAGIDVRPYAKTLIGSDLSPRMIARARERKLYDELHVEDLVTTLTRERDVDL
                     VVATDVFIYVGALEATFAACATALRTVGLLAFSVERSDGEDVILGSTLRYSHSDGYIR
                     RLADAHGLTVLRAEPTTLRVEEAKPVAGVLYILQR"
     gene            11931..13025
                     /locus_tag="VMZ53_29145"
     CDS             11931..13025
                     /locus_tag="VMZ53_29145"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HUS32616.1"
                     /translation="MMGSFARVLGAMLVAGCMVGEPGSAPVSEEPDSASETAEEAPAQ
                     TTPIALTVSDGLDTEERIFLQKLNDYRAANGLAPLQVSVALTQASDFHSRDMATKSYF
                     SHDSQDGTTWSARIRRYYDHNTYLAENIAAGNAGGDATFTQWKNSPGHDANMKGASYR
                     VIGIARAYDAAATYRWYWTTDFGGYVDEVMTPDATTPPDPMQPPPPPPMTTNVLVNGD
                     FAANDIGAGVVYTNVRQTGRWYAWAGSGSATIQSGVLRLYDALTGYISVAQLVAAAPG
                     KIYDLKANAAWVSGSTGQVIYLDFLDSSYQRIGRSTISAGSSSTLHQLTRSVTAPAGT
                     AFARVTLYGYAGNNDYSSTYTWDDLSLAPR"
     gene            13167..14036
                     /locus_tag="VMZ53_29150"
     CDS             13167..14036
                     /locus_tag="VMZ53_29150"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS32617.1"
                     /translation="MRLVALIALAGCTAPALEEDVVYDDRLGESGLMDIYMPDDAGPH
                     PAVLFIHGGTWRAGSKEHFRRGGARLARSGYVAASINYRLLPDGVFPNNLNDCVCALA
                     FLRAHATEYSIDPDRIAVMGYSAGAHLTSLVALASDHPELPTDCAAANGQPVAPPAAA
                     ISASGPQDMVLWWNEMDDKETVEGIFGGSLAEKPHAYDLGSPRYHVKAGAPPFLILHD
                     ALDFGGAHAFRDALIATGNAASLLVVEGSLHILEQQDDPGFYEGGVSSETPVAWIAIE
                     DFLSRTIGGGAWH"
     gene            14036..14533
                     /locus_tag="VMZ53_29155"
     CDS             14036..14533
                     /locus_tag="VMZ53_29155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HUS32618.1"
                     /translation="MRALLVLVLLTGAAHAEPDVIATQPLALAANGVSASYERPLTTK
                     LSGVAIAGYRDGADGDFDANTFTGGAELRRWFRDDGRMRGPFIGVHVSVGHTRLSDQM
                     GYVGSSTSFTQRLEFGWRFVIRNHVTIAPAIGIAIHEDISGTGNLAPIGHAMPGLGLE
                     LGWMR"
     gene            14559..15260
                     /locus_tag="VMZ53_29160"
     CDS             14559..15260
                     /locus_tag="VMZ53_29160"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HUS32619.1"
                     /translation="MRIGAAVLGFAAACSFSHGSLTQPDPDATAVDSSSTDAAIDARP
                     DARPDSPPAQPVTATFADVADTYTDSANPTTAFGGTASMYADGSTTPATALVRFDLSA
                     IPTTAVVQSAVLHVWVSNDIGAAVQVYQMLEGWDELTATWNQRMTNTAWTGAGASPPS
                     RGTTMLASFTPDAFYAEKTGTIASSVVQSWVTTPALNYGFAITTQDADGSAWRTRENT
                     TIENHPILVVTYVPQ"
     gene            complement(15254..>17821)
                     /locus_tag="VMZ53_29165"
     CDS             complement(15254..>17821)
                     /locus_tag="VMZ53_29165"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HUS32620.1"
                     /translation="TQDYAKEKPHLDKIAALYDDLRKPGADADKILKEIDSETQAAAA
                     FAIPNELDQMYSQLGIGGLNAFTNSDSTVYVTEIPKNRIEQWAHVEADRYDDAVFRLF
                     WPELEAVYEEKNRGMDSPERRVRDAFMKSLFPNHGYGWSSVLGEVEHLKKPAYGDMTR
                     FFTRYYTPGNMAIMLAGDVDESVLPLLEKEFGKFKRPAGPAAEPGTIAPVAKRTELSV
                     KVPSDEGVLLGWTLVSATHPDRLPLELMDLLLLDGRSGILARDLLITQKVANAGCNPT
                     FLRDGGYYELYADSLDGQTHEQLEKMLLELVGKLQRGEFSDADVSTAILTYEINRQRQ
                     LESNGGRMGMLLDSFVNDEDWGDTASRIERMRKITKADIMRVAKQYLSPSYLVVKKVK
                     GQESPPKITKPGITPVKMQQGLRSAYAQSILDMPVKPIDAVSLVEGKDYTRAKIATGD
                     LVSVKNDRNGLMSVTFEYNFGRIDDKWSCLALETLKNTGAGKRTPEETDRALHELGLS
                     IDTGCGKEGSSITISGIDRNLDEGMKLLREWLAAPALDDKILAAKVNAALTERANMIT
                     NPRVIAGAAQAYAQFDKNTEYLVVASNKDLKAVKPDQITKTLKGYLSRKHRTSYFGPR
                     DPAQAATAVVLGDGKTPMRADHVVKYRKPGNVYVTSQETAQTQVWIVWPRQPSNDNDR
                     AAGQLFSEYIAPYLFQHVREARGLAYTVYGFYGASGRTKDDSEVAAFVGTQGDKAHDA
                     VDAVLEAMKQPFDDARLALAKKTIEESYRVERIPPRAVANYVYSWDDQGLKSDPREAR
                     TKRTLAVDKATLEKWTKAAFAGKTTVSMVGDKKKLDDAKLKKLGPVTDVPISKLFGY"
BASE COUNT         2979 a         6052 c         5667 g         3123 t
ORIGIN      
        1 cgcgatcgcc gagccggcac cggagcgacc cggcctcggc accacgttcg gcgagcaggt
       61 gtatgcgccg atcagcttcg ccccattcac ccgcgcaagc agtgcgccct gggccgaggc
      121 cgcgcttcac tacaacgatg ccgacggggt cgaggcccac gcgcgctacc tcggcacgcg
      181 cccactgcca ctcgagctct acgccggcga cggatcgatc tcggtcgcgc tcaccgacga
      241 ctacggccac accctgcccg gcctcacggc ccagggacgc acgatgatcg tcggcgagga
      301 cggccagcgc tacaagatcg tcgtccgcaa cgcgaccacc gcgcgcttcg agatcgtcgc
      361 gtcggtcgac ggggtcgacg tgatcgacgg caagcccgcc gatccgaacc gccgcggtta
      421 catcatcgac ccgcacgaca cgctcgtgat cgacggcttc cgcacgtcgg atgcgaacgt
      481 cgctgccttc cggttcggtc gcgtagccga ctcgtacgcg gcgaagtcat cgggagatcg
      541 caacgtcggc gtcgtcggtc tcgcgatctt ctcggagcgc ggcgcggtct ggacgcgtgg
      601 agagctgcag cgtcgcgatt cagcgaatcc gttcccctcg cggggctacg cgatgccgcc
      661 tcggtaacgt gctagcgttt ccgccgggtg ccttacaacg acgtatttca cccgggctcg
      721 acgctcggca agtacgagat cgttcgcgag ctcgcgaccg gtgggatggc gcagatctat
      781 ctcgccaaag tgacgggcac ggcgggcttc gagaagaacg tcgtcctcaa gtgcatcctg
      841 ccgaatctgg cgaacgaccg gcagttcgtc acgatgttcc tcgatgaagc gcgcctcgcc
      901 gcaacgcttc gccacagcaa catcgcagac gtgttcgacg tcggcgtaga aggcaagacg
      961 tattacttcg cgatggagta cgtcgcggga cagaacgcac gcacggtccg actgaaggcg
     1021 cgcgaggcga agcggccgat tccgatcgag atatcgctgg cgatcgtcgg cggcaccgca
     1081 gccgcgctgc actacgcgca cacgcgcacc gatgcgaacg gcaagtcgct cgacctcgtt
     1141 caccgcgacg tgtcgcccag caacatcctc gtcggctacg aaggcgccat caagctggtc
     1201 gacttcggca tcgcgcgcgc cacctcgcgc cgcctccgca cgcgcacggg cattcgcaaa
     1261 ggcaaggtcc cgtacctgtc gccagagctg tgccggggcc accacatcga tcaccgcagc
     1321 gacctgttct cgctcggcac gattctctac gagctgacga ccggcgagcg gccgtttctg
     1381 ggcaacagcg acttcgtcgt gatggatcag atcgtgacgg gcacgccgct gccgccatca
     1441 tcgacgacga agggctatct cgcagggctc gagccgatcg tcatgcggct gctcgctcgc
     1501 tctcccgagg cgcgctacca aaccgccggc caggtgctcg aagacatcga gacactcgcg
     1561 tcggagaacc ggctcctgat ctcgtcgcac attgtcgcga agttcatgaa ggagctgttc
     1621 atcgatgcgg gtaccgcgcc gttcgagcgc gacacctact tggacttcgc gcagcccgtc
     1681 gtcgacgacg agaagcacgc ggatgaagaa ccgaccgccg cgttcagtcg ggcggagcag
     1741 atgtggaccg ccgagctgaa ggtcaaagtg cggacaccac ggcgcgcgca gtcagagccg
     1801 ggcgaagaga tcagcattac gtctgccgtc ttccacgagc cgacgcgcca agatgtcgta
     1861 ccggcgttcg atccgatcga tgcgcgcagc gaagaaatct tgatccagct ggacgccgaa
     1921 gcgcccgatg acgagtcgcg agaccgtcgc gctcatcgcc gcatcatcac gttgatcgat
     1981 cgggcgctcg agtgggtcga tagcggcgag ctggataagg ccgtcaccgc ggtcgagctc
     2041 gctctcgacg aggatatgtc cgtccccggc acgcaggacc tcctgcaaga tcgcgtcgca
     2101 tcgatcgtcg ccgtctacga ggcgatgctg gaggatccgt atcgcgtgct cgagctcgcg
     2161 cggccgatcg atgagctcgc tggcgtcgcg atggagatcg gtgctcgcac acttcttgcg
     2221 tggatcgatg gcaagaccaa tatccagcag ctgctcgcac acgctgaaat gccgcgttta
     2281 gaggcctatc accacctgtg ccaactgttg atgcgcggga tgattcgcta gttgtaacaa
     2341 ccactgcgca gcatcgtggt atccggaccc gcgatgccga cgtacgacga cgagctgctc
     2401 gcgccgatcg atacgcttcg cggctacgac cgctgggcac ccacctacga tcacgatccg
     2461 aattccgtgg tcgccgcgac ctcctgggtc ctcgaccgcg cgcccctggg cgtagcggat
     2521 gccgacgtgc tcgagcttgg ctgcggcacc ggccgcaata tcccgcgcat cctcggcgaa
     2581 ggagcacgca gctacctcgg cctcgatggc tcgcacggca tgctcacgat cgcagcccaa
     2641 cgctatcaag atccgcgcgt cacgttcggc gtcacggatc tcaccgcgca atgggcgctg
     2701 ccgaagcagt tcgacttcgc gctgatcgtc ctcgtgctcg agcatctgcc cattctcgat
     2761 ccgttgctcg tctcgctggc gcgcgcgatt cgccccggtg gtcgagttcg catcgtcgat
     2821 ctgcatcccg agcgcatcgc ctcaggctcg ttcgcgcatt tccaagacgg cacgacgagc
     2881 gtgcacttct cgagcgtcgc gcatgccgtg ccgatgctct gcgccgggct cgaagctgtc
     2941 ggcttcgaga gcgtgcgtcg cgattggctc gccggcgaga cgatgctcgc cgaggtgccc
     3001 ggcatctcga agcatcgagg cacgcgcctc gtgctcgatc tcaaggcgac acgtcgttcg
     3061 cgcgaccgcc gcggctccgg ctcgctctaa cgcgaacgag agcgtgacgc cgcacgggtc
     3121 ttgccgcgac tcgctgcgcc gccgacgcgt ccgcccttgc gatacggcgc cttgttctcc
     3181 ggcttgccgc gaccgcttcc ggacttctcg ccgccaccgt ggatcgcgtt gacggtcgcc
     3241 catgcgcggg ccttcgcctc gcgcgcggag acgccgcgct ccttgtatcc ctcggcgatg
     3301 tgctcggcct tgcgcttctg cttatcggtg tatttggtct tatcgcctct gggcatgatc
     3361 gagatcgcag caagtgtcgc gccaccgctc acccatgatg gggtgcctca ggcccgtaat
     3421 ccggtgagtc agacgagcgc gggcgcgcgc attggcacag cggtcgtcgc gagagggcac
     3481 ggtttccctg cggaattgcg aaggtcacgg aggggcacgc cggctgcaca aagcttcgcc
     3541 gcaatgcccg atctcgatac ctcccggatt ccgcgtgagg ccttgatcgg cctgctctcg
     3601 gaatcgagca acatcgaccg cgagcgcgtg acagcaccga tgccagtcgc acgactcgac
     3661 gagcttctcg gcgaggacaa gatcgaagag gtcctcaaca gcgacaaccc cggcgcggtg
     3721 gtcgaagaag ccgagccgct cgcgaccggg agcaatgccg agctcatcgt tcgcttcaag
     3781 cagcagcagc agcctgtgac gagcacggtg atgtctcacc acgtgatcgt gtggggttgc
     3841 gccgtggcgc tgctcatcgt cgctctcatc ctcgttctgg tctgatctat ggacacgact
     3901 gccgacaccg ccatccgcga caccctcgag atgccgccga cgcagctgga gtcgatcctg
     3961 gtgcacgaac gcaccacggc tgaggacgtc gtcatcgagc tcacgaagcg taagcgaaag
     4021 ccgctgcaaa cgattcacct cgtcgcgatc agcttcatct ccggcatcgt gctcggcctc
     4081 gccgccgtcg ccgtggtcta gccgtacagc gccgcaaccg cgacgatcgc cgtgaagcga
     4141 agcgcgcgcc ccaccgcgat gaccgcgcag aacgtccgcg gtggaatcgc gagcgctcca
     4201 gcagccgtcg agataatcga gaatggcggc agtcctagca gcgcgctggt gccgagcacg
     4261 aggttcggcc gattaccgaa gcgtgcgacc cacacgcgga cgcgctcgat ccagcgccgc
     4321 tgcggtcctg gcaacgcggc aagcccgcgc gtcgtcgcat agaccggcag cttgccgagc
     4381 acctgcccca ccgtcgcgag cacgatcagc gggaccgcat tcgccgtccc atgcgcgagg
     4441 gtgatcgcga cgaggaagac ctcgatgctg atgagcggga tgacgctgct gatcgcgccg
     4501 accgcaaacg tcccggcgta gatgccagcc gctcgcacga tctcgtctgc ggtcatcacg
     4561 caacccagcg atagaccgcc ggtgcggcga ccgcgccgat cgcgtcgatg gcggtcacgg
     4621 ctgcaaccag ggcgagcccg cgaacgacag ggccgcgccg catcgcagac agcgcaccgg
     4681 tcaccgcgcg catcgcaaag atggcgagcg caggcagcgg cccaagcttc tcgaccgtcg
     4741 agcgcgtgcg tggcgcatac gtgccgagca cgtgcggcaa gagctgcgtc gtgtccgcgc
     4801 cgcgcaagag tcgcacctcg aggaattcac ggaggtcgtc gggccacgcg tgggtgacgc
     4861 gcgcgtcggg cgcataagcg atcggaatgc ccgcttccat gaagcgaagc gcgagcactt
     4921 ggcactggcc gtgaaacatc ggctcgatcg tcggatagcg gcgcgatgcg aaggcctcgc
     4981 gcgcaaacgc gacgttgttc gcgaagaagt tgcgaacctc gggcgctgtc ttcgccgacg
     5041 tggctgcgaa gctgcggcgt ttatcggcgt actcgaagta cggaaagtcg agctcgttcg
     5101 cgagcggcgc cagcacgccg tcgtatgaag tcgcgcccgc gacgacctgc gcgtgattac
     5161 tgaacggcgc gacgagcgca gcgagccagc cttcgctcgg ttcgcaatcg ccgtcgataa
     5221 acgcgacgac gttgccggtt gtcgcgtcaa aacccgcgtt cttgtggtcg tagtatcccg
     5281 cgcggccgtc gagctgcacc cagtggatgc gtcgcccgtg acgttgttcg agcgtttcgc
     5341 gcgttccggc actaaggtcc acgtacgtga cgacgagctc ggcatcgaga gccgagatct
     5401 gcggcgcgag acgatcgagc tgccgcgcga gcgtcggcac atcgcccttc gtgttccacg
     5461 tctcgacgac gacactcacg cgcgtcacga cgcggcctcg acgagctgcg cgcgttcggc
     5521 agcgaccttc gcgtagatct cccacgtcgc acgcacggcg gccgcgaggt cgtagcccgc
     5581 tgcatgcgcg cgagcggcag cacgcatctg ccaacggcgc agcggatcag cgaacagctc
     5641 gagcaccgcg tccgcgagct cttgcgactt cttgccgtcg acgacaaggc ccgtctcgcc
     5701 cgcgacgaca tgctcgtgcg atgcgcctgc gcgcgcgacg acggacggca ggccgctcgc
     5761 cgctgcctcg acgacgacgt taccaaacgt ctctgtctcg ctcgggaaca tgaacacgtc
     5821 cgccgacgcg aacacgctcg cgagctcctc gccgaacagg ccgcccgtct ccaccatgcc
     5881 gggcgccttg aacgtgccgg cgagcgggcc ctcagccccg acgacgagca gcttcgcgtc
     5941 gggccgcgcg gcatggacct tggcccatgc ggacgtcagc gcgtcgagat tcttctcctt
     6001 cgagaggcgg ccgacgtaga gcgcgaccgg cacatcgccg atgccccact tcgcgagcac
     6061 accgcgatcg cggcgcgccg ggttgaacag ctcgagatcg acgccgcgtt ggatgcgcca
     6121 gatgcgatcc gcggcgatgc cgaacgatgc gaggcgttgc gcgaccgctt gcgacggtgc
     6181 gagacagacg tccgcttgct tgtagaacca gccgacgatc ggctcgacga tcatcttcac
     6241 catcggcagg cccgtcatct gcgcggcgta gtcggcaacc tctgtgtggt actgcgcgat
     6301 gacgggcaga ccgagcatgc gcgcggcgat gagaccggca attcccatcg ggcccggcgt
     6361 cgcgacttgc acgatgtccg cattgcgcgc gagccacgac gtgagcggcg gcagctccgg
     6421 cacgctccac gtcatatccg gatagatcgg caggctcgcg gtcatcgctg agggaatgcg
     6481 cacgacctcg tcgacgacgc ctggcttcgc cgctccgacc aagtgcacct cgtgtcccgc
     6541 acggcgtcct gcagcgacga gccgccgcaa gccgacgcaa atgccgttga tgtcgtcgac
     6601 cgtgtcgctc acgatcgcta cgcgtagccc gcggctcgtc gtcctcatcg ggcacctcca
     6661 cgcagcctgt cgcgtgtcgc gtagctcgcg atcatgagcc agaacacgac cgagatcgtg
     6721 acgccgaacg cgcgatcgat cttcgcgatc ttctcgcgca gctcttcgag ccgcgtccgc
     6781 tcgtgatcat tgaccagtcg atcgacagcg agcgcgcagc tcttcgcatg tcgcttttcg
     6841 tcgctcagga tctcgcgcaa cgtatgcgtc gtcggatcat cagcgcagct ctgctcgagc
     6901 acagcgaggt ggcggccgaa catccgcacg cccgtgcctt cgagctgcgc cgcgatcgcg
     6961 agaagcacga cgacgggccc tgcttcgaac gcatccatat atggcgccgt gacgcgctcg
     7021 acccaccaca gctttgcgcg catgaggctt ggcggctggc gatccgtcgg tgcaccgagc
     7081 gcgacgagac gctcgcgcaa cagcgctgcg tggcgttgct cgtcggcgag ctggttcgca
     7141 gcaagcttgt cgacccatgg cggcgtcgtc gacgtcgacg tgcgatgaag cgcggccgtc
     7201 tcggcaccct cctcgctcca cagatattcg gtgaggatgt gtgcttgtgc gcgtggcgag
     7261 ccaagcgaca tcacgagtgc gtgacgcttg atctcgtcca ccgcgcgacg ctgaagctcg
     7321 cggatggtca tgccgattca cagagcaacc agcattcctg cggcgttggc cgaaccactg
     7381 ccatccactg ccgtggtttc cacggagtga aggcgcgtcg tcgacggata cctgacgctg
     7441 ccgatgaacg cctggtagct gcctatcgca tcacttgaca gtgaaatgcg atcgcgctcg
     7501 caggcgttct ttaccaggca accaaagcga gctacgcgac gtcgatctgc tgccaacccg
     7561 agtcgggatc gttgccggcc atatcgaggc cagcttggat ttcccacgtg tggttgatca
     7621 gcgatccgcg aagcgtcgcg ttcacgttgt gcggcagctc gagctggcca gaccagtcat
     7681 acgtctggcc ttccttgagc tcgcacgcgc cagcgatctg gatcttcgtc tcgaagctaa
     7741 ccttcgagtt cgagaccgtc gtgttgttct cgcccttcca cgacgccgtc tccatgccac
     7801 gaacgagcag gtacaccgag ttgaccttgc cgttcgcctt ggcttgcgcc tgaacttgta
     7861 ccggcactgc agcgccgcga cgcacaccct gcgtcgacac gcgaaccgtt gcaccaccgc
     7921 cagtgacctt gttcttcaaa tcgctgaaaa aacccatctg caatctcccc tgttgagaaa
     7981 cgaagccgcg aatttacgcg ggctcgccat gtaaaagaag cccaggccgc cactcccgac
     8041 gacgaggacg aaaattccag tcctgattta ttggacgtcg aacgactgcc agcccgagtc
     8101 cgggtcgtta cccggcatgt cgaggcccgc ttggacctcc cacgtgtgat cgatgatcga
     8161 cccacgtagc gacggcatcg cgttctgcgg cagctcgagc tggccttccc agctgtacgt
     8221 ctggccttcc tgcagctctt gcgcgcccgc gatcatgacg cggttttcgt agctgacctt
     8281 cgagccgctg accttctcgt tgttcgagtc tttgaactgc gcgttctcgg tggcgcgcac
     8341 gagcaggtac accgcgctga ccttgccgtt cgtcttcgcc tgcgcctgga cctgcaccgg
     8401 gattgcagcc ccgcgccgac cggccggcac gctcacgcga accgtcgctg caccacccgt
     8461 gatcttgttc ttcaaatctt tgaaaaagcc catgttcgtt ctcccttgtg aggagacgag
     8521 ccgcgagccg ggcgccagtc gcaaagaaaa tgtgctagaa gatggggcct tcggagccat
     8581 cgccaagtgg taaggcaacg gtctgcaaaa ccgtcattcc ccagttcaaa tctgggtggc
     8641 tcctccaaat cgcagtaagc gacgtatctc gacgcacaat cgttgcagag cggcgtctcg
     8701 agcccgccga tcccgacgtc gggcgcgccg ttcgtcagtg tacgcttccg ccgaatggtg
     8761 acgagcgtca gccaacagac agcgccgctc gcgcctcctg ttggtgaagg cgtcgtcggc
     8821 gactatgcgc tcgtgcggcg gatcggccgc ggcgcgatgg gcgaagtctg gctcgcgcgc
     8881 cacacgcgca cgggcggcat cgccgcgatc aaagttctcc gcgatggcac cgcgcgcaac
     8941 cgcgagcgtc tgcgccgatt cttcgcacgt gagcggcgcg cggtcgctcg tctctcgcac
     9001 gcgaacatcg tcccgctctt cgagctcggt cccgactaca tcgcgatgcg gtacatcgac
     9061 ggtccgacgc tggcgcgccg actcgcgacg ccgatcgaac ctgtcgctgc gctcgacatt
     9121 gcggtgcaga tcgcgtcggc tctcgcgcat gcgcacgcgc gtgatgtcat ccatcgcgac
     9181 gtcaagccat cgaacatcct gctcgatacg atgggcaacg catacctcgc cgacttcggg
     9241 ctagcggcgc tgctcgatga cgcggacgct ggcgacgagc gaggcggtac gccgcagtac
     9301 atggctcccg agcagacgcg cggcgacacg ccatcgccgg ccgccgatca attcgcgctc
     9361 ggctgcgtgc tcgctgaaat gctcgccggt ggccgcgtcc aagccgatcc ggatgccgtc
     9421 gcgagtgcgc tgccgcagac gctgccgcgc aggctcgtcg atatcatccg gcaagcgatc
     9481 gatccggatc cggcgaagcg gtggccatcc gtcggaagct tcgcgatcgc actgcgcggc
     9541 ctcgacgtcg cgagctcgcc cgttccgcag cgcttgctgc cggagctgcg cgtcagcggt
     9601 ccgttcgcat gggccgcaca tcaagacgca ttcaccgagc tcgcgccgtc gatcagtcgc
     9661 gcggactacc ggctctccgc gctcgaagcg aagggcgcga tatcgccggc ggccgcgcgc
     9721 gcgtttcgcg aacgcacggg ctacgcggac ttcggttggt cggtcgtcgg gcgcacggat
     9781 cggctcggcg cgatcacgga tccgcgagcg tacgcacgcg ccggcgaggc ggtcgtcatg
     9841 atccacggcg gctttgcaac gcgcgagtca tggatcagcg tcgcgacgcc ggtcgcgcag
     9901 ggcaatggcc agacgctcgt gttcatcccg gacttatccg gctacggcgt gtctcgctat
     9961 caggagcgca gacctgcgca gcagcaagtc agcccgcaag gctcgctcga cggcgtcctc
    10021 gcctggatgg agctgatcgg agtgcgcgag ttgccgctcg tgctcgtcgg gcactcgctc
    10081 ggagccaccg cgctcatgac ggccagcgac gatgcgctcg gtgcacgaac gtggcgtgtg
    10141 gcggtagcgc cggtgttccc tgccgtcgac tggcggcagc ggtggggact gcggctcgcg
    10201 cccgcgatgg gcctgtccct cgcgtggatt cctggcgtga agcggctggt cgcgaacatg
    10261 acgttccagc tcgcgcccgc cgcgcgcgcg tacgagcccg aggaacgcca gcgcatgctc
    10321 gaagcattta tggcgcttcc cgcgccgacg ctgatccgca gctcccgcca atactcgtat
    10381 gcgaggcctg cgccgagcac agagctcgca cgctgcgtcg tcgtgctctc cgagaacgat
    10441 ccagtcgcgc cgatgcagcg catgctgcgt gcgctcgccg agctcgagtt ccccgagcat
    10501 cagatccatc gcctcgtgtc gcaaggccac ctgcctcacg ggcagaccgc gatcaatccc
    10561 gagtggacgc agcgcaacat cgtcgagctc gcggcgatca tcgatcagct gctcgtcgcc
    10621 gcgcgcgaag gctccgtgct gccgacgcag gtcgcttcga cgctcatgtc cgatggtacg
    10681 tcgacggaat cggcgtgaag cgcaagaagg acaagcggac gctcgacgac gtcgtcgatc
    10741 tcgggcgaag ccacgtcgat cgcggtgagc tcgcgcaggc agaagcttgc tttcgtgaag
    10801 cgctcgcgat ggagcccgag cacctcggcg tcatcacgtt gctcggcttg ctcctcgtcg
    10861 aacgcgagaa gttcgagccc gcgatcgacc tactcgagcg tgcgcgcgac gcggccccgg
    10921 agttcgcgcc ggtccagctc gcactcggct cggcgtatgc gggcgcaggc cacgatgatc
    10981 tcgcggtcgt cgcgatggag accgcgatca agctcgacac gacgagcacc gtcccgctcg
    11041 agcgactcgc gaagcactac attgtcgcgc gcaagccgcg tgaagccatc ggccttctgc
    11101 gccgcgtact gcgtcgcgat ccgacgcacg cgcacgcaca gttcctgctc gcaggtttga
    11161 caggcgacca gccgggggcc gcaccgcctt cgccgccggc cgagctcatc gccgatctgt
    11221 tcgatgccta cgcgacgaag ttcgacgagc acctcgtcgg tgggctgcag tactcggcgc
    11281 cgaaggcact cgccgcgttg gtgagtgaga ccggagcgaa agcagacggc acctggcgcg
    11341 tcgtcgacct cggctgcggc accgggcttg ccggcattga cgttcgcccg tacgcgaaga
    11401 cgctgatcgg ctcggaccta tcaccgcgga tgatcgctcg cgcgcgagag cgcaagctct
    11461 acgacgagct gcacgtcgaa gatctcgtca ccacgctgac gcgcgagcgc gatgtcgatc
    11521 tcgtcgtcgc gacggacgtg ttcatctacg tcggcgcgct ggaggcgacg ttcgcggcgt
    11581 gtgcgaccgc gctgcgcacg gtcggcctcc tcgcattctc ggtcgagcgc agcgatggcg
    11641 aggatgtcat cctcggttcg acccttcggt actcgcactc ggacgggtat atccgccggc
    11701 ttgccgacgc ccacggcttg acggtgctcc gcgcggagcc cacaacgctg cgcgtcgaag
    11761 aggcaaagcc cgtcgctggc gtgctgtaca tcctgcagcg ctgacgcagc gcagctcaca
    11821 ttttgttggt gctcaaaaga atcacggcga cgtcgaggtc gcctgtcaga cagcgcccgt
    11881 tagcttgttg gggctgagtc aagaagccag accgtctaac gtcgctcacc atgatgggct
    11941 ctttcgcacg cgtgctcgga gcaatgctgg tcgcgggctg catggtgggc gagccggggt
    12001 cggcgccggt ctcggaagag ccggactcgg caagcgaaac cgccgaagag gccccggcgc
    12061 agactacgcc catcgcgctc acggtaagcg acggcctcga caccgaggag cgcatcttcc
    12121 tgcagaagct gaacgactac cgcgcggcca acgggctcgc gccgctccaa gtttccgtcg
    12181 cgctcacgca ggccagcgac ttccattcgc gcgacatggc gacaaagagc tacttctcgc
    12241 atgacagcca ggacggcacg acgtggagcg cgcgcatccg tcgctactac gatcacaaca
    12301 cgtatctcgc cgagaacatc gccgccggaa atgccggagg ggacgcgacg ttcacgcagt
    12361 ggaagaattc acccggccac gacgcgaaca tgaagggcgc gagctatcgc gtcatcggta
    12421 ttgcgcgcgc gtacgacgcc gcggcaacgt atcgctggta ctggacgacg gacttcggtg
    12481 gctacgtcga cgaggtgatg acacctgacg cgacaacgcc acccgatccg atgcagccgc
    12541 ctccacctcc gccgatgacg acgaatgtgc tggtcaacgg cgacttcgca gcgaacgaca
    12601 ttggcgcagg cgtcgtgtac acgaacgtcc gccagaccgg acgctggtac gcgtgggccg
    12661 gcagcggctc ggcgacgatc cagagcggcg tgctgcgcct ctacgatgcg ctgaccggct
    12721 atatctccgt cgcgcagctc gtcgccgcgg cgcccggcaa gatctacgac ctgaaagcaa
    12781 acgccgcctg ggtctcgggc agcacgggcc aggtcatcta tctcgatttc ctcgacagct
    12841 cgtatcaacg gatcggccgc tcgacgatct cggcgggcag cagctcgacg cttcaccaac
    12901 tcacgcgctc cgtcacggcc ccagcaggca ccgcgttcgc gcgcgtcacg ctgtacggct
    12961 acgccggcaa caatgactac tcgtcgacgt acacgtggga cgacctgtcg ctcgcgcctc
    13021 gctgagccag cgccggatcc cgagacaacc gggcggaaac cccggcaggc agcggcactt
    13081 gccagatttt cgacgtgaac ggcgggccac acctgtgggc tttgccgacg cttttgcgcg
    13141 gcacgacgcc tgctcttgcc aaggcaatgc gcttagttgc tttgatcgcc ctcgccggct
    13201 gtaccgcgcc ggctctcgag gaggacgtcg tgtacgacga tcgccttggc gaatccgggc
    13261 tcatggatat ctacatgccg gacgacgctg gccctcatcc tgcggtgctc ttcatccacg
    13321 gcggcacatg gcgcgccggc tcgaaggagc acttccgccg cggcggtgcc cggctcgcgc
    13381 gctcgggcta cgtggccgcg agcatcaact atcgcctgct gcccgatggc gtcttcccga
    13441 acaatttgaa cgactgcgtc tgcgcgctcg catttctgcg cgcccacgcg acggagtatt
    13501 cgatcgatcc cgatcgcatc gccgtgatgg gctactcggc cggcgcgcac ctcacgtcac
    13561 tcgtcgcgct cgcgtcggat catcccgagc tccccacaga ctgcgccgcg gccaatggcc
    13621 agcccgtcgc tccgcccgct gccgcgatca gcgcatcggg accgcaagac atggtgctct
    13681 ggtggaacga gatggatgac aaggagaccg tcgaaggcat cttcggtggc tcgctcgccg
    13741 agaagccgca cgcgtacgac ctcggctcgc ctcgttatca cgtgaaggcc ggcgcaccac
    13801 cgttcctgat ccttcacgac gcgctcgatt tcggcggcgc tcatgcattt cgcgatgcac
    13861 tcatcgccac tggcaacgcc gcgagcctgc tcgttgtcga aggcagtctg cacattctcg
    13921 agcagcaaga cgaccctggt ttttacgaag gcggcgtgtc gagcgaaacg cccgtcgcat
    13981 ggatcgcgat cgaggacttt ctatcgcgca cgatcggagg tggagcgtgg cattgatgcg
    14041 cgcgctgctc gtactcgtgc tgctcaccgg cgccgcacac gccgagcccg acgtgatcgc
    14101 cacgcagcca ctcgcgctcg cggccaacgg cgtatcggcc tcgtacgagc gaccgctcac
    14161 gacgaagctc agcggcgtcg cgattgcggg ctaccgcgat ggagccgatg gcgacttcga
    14221 tgcgaatacg ttcacgggcg gagccgagct gcgacgctgg tttcgtgacg acggtcgcat
    14281 gcgtggaccc ttcatcggcg ttcacgtcag cgtcggtcat acgcggctct ccgatcagat
    14341 gggctacgtc ggctcgtcga cgtcgttcac gcagcggctt gaattcggat ggcggttcgt
    14401 gatccgcaac cacgtgacca tcgcgcctgc gatcgggatc gcgatccacg aggatatttc
    14461 ggggacggga aacctcgcgc ccatcggcca tgccatgccg ggactcggcc tcgagcttgg
    14521 ttggatgagg tagctgcgtc gcgtggtaag ctcggctcgt gcgtatcgga gctgcagtac
    14581 ttggcttcgc tgccgcgtgc tcgttttcgc acggctccct cacgcaaccg gatccggacg
    14641 cgacagccgt cgacagctcg agcaccgacg ccgcgatcga tgctcgcccc gacgcgcggc
    14701 ctgattcacc gcccgcgcag ccggtgaccg cgacgtttgc ggacgtggcc gatacgtata
    14761 cggactccgc gaacccgacg acggcgttcg gcggcaccgc tagtatgtac gccgacggat
    14821 cgacgacacc tgcgacggca ctcgtgcgat tcgacctgtc cgccattccg acgacggccg
    14881 tcgtgcagag cgctgtgctg cacgtctggg tcagcaacga catcggtgcc gcggtccagg
    14941 tctaccagat gctcgaaggc tgggacgagc tgacggcgac ctggaaccag cgcatgacga
    15001 acactgcgtg gaccggggcc ggcgcatcac cgccgtcgcg cggcacgacg atgctcgcat
    15061 cattcacacc agatgcgttc tatgccgaga agaccggcac gatcgcgagc tccgtcgtgc
    15121 agagctgggt gacgacaccc gcgctcaact acggtttcgc gatcacgacg caggatgcgg
    15181 atggctcggc gtggcgcact cgtgagaaca ccacgatcga gaaccatccg atcctcgtcg
    15241 ttacatacgt tcctcagtag ccgaagagct tcgagatcgg cacgtcggtc accgggccga
    15301 gcttcttcag cttggcgtcg tcgagcttct tcttgtcgcc gaccatcgag acggtcgtct
    15361 tgcccgcgaa cgcggccttc gtccacttct cgagcgtggc cttgtcgacg gcgagtgtgc
    15421 gcttcgtgcg cgcttcacgc ggatccgact tgaggccctg gtcgtcccac gagtacacgt
    15481 agttcgcgac ggcgcgcggc gggatgcgct cgacgcggta gctctcttcg atcgtcttct
    15541 tcgcgagcgc gaggcgagca tcgtcgaacg gctgcttcat cgcttcgagc acggcgtcga
    15601 cggcgtcgtg tgccttgtcg ccctgcgtgc cgacaaacgc cgcaacctcg ctgtcgtcct
    15661 tcgtcctgcc ggacgcaccg tagaaaccgt acacggtgta cgcgaggccg cgcgcttcac
    15721 gcacgtgctg gaacaggtac ggcgcgatgt actcgctgaa cagctggccg gccgcgcgat
    15781 cgttgtcgtt cgacggctgg cgcggccaca cgatccacac ctgcgtctgc gcggtctctt
    15841 ggctcgtcac gtagacgttg ccgggcttgc ggtacttcac gacgtgatcg gcgcgcatcg
    15901 gcgtcttgcc atcgccgagc accactgcgg tcgccgcttg cgctggatcg cgcgggccaa
    15961 agtacgacgt gcggtgcttg cggctgaggt aacccttcag cgtcttcgtg atctggtcgg
    16021 gcttcacggc cttgagatct ttgttcgacg cgacgacgag gtactcggtg ttcttgtcga
    16081 actgcgcgta tgcctgtgcc gcgcctgcga tcacgcgcgg gttcgtgatc atgttcgcgc
    16141 gctcggtgag cgcagcgttg accttcgcgg cgaggatctt gtcgtcgagc gcgggcgctg
    16201 cgagccactc gcgcaacagc ttcatgccct cgtcgaggtt gcggtcgatg cccgagatcg
    16261 tgatcgagga gccttcctta ccgcagcccg tgtcgatcga gaggccgagc tcgtgcagcg
    16321 cgcggtccgt ctcttccggc gtgcgcttac ccgcaccggt gttcttcaac gtctcgagcg
    16381 cgaggcacga ccatttgtcg tcgatgcggc cgaagttgta ctcgaacgtg acggacatca
    16441 ggccgttgcg atcgttcttc accgagacga ggtcacccgt cgcgatcttc gcgcgcgtgt
    16501 agtccttgcc ttcgacgagc gagaccgcgt cgatcggctt cacgggcatg tcgagaatgg
    16561 actgcgcgta cgcgctgcga aggccttgct gcatcttcac gggcgtgatg ccgggcttcg
    16621 tgatcttcgg cggcgattcc tggcccttca ccttcttcac gacgagatag ctcgggctca
    16681 ggtattgctt cgcgacgcgc atgatgtccg ccttcgtgat cttgcgcatg cgctcgatac
    16741 ggctcgcggt gtcgccccaa tcctcgtcgt tgacgaacga gtcgaggagc atgcccatgc
    16801 gaccaccgtt cgactcgagc tggcgctgac gattgatctc gtacgtcagg atcgccgtgc
    16861 tcacgtctgc gtcggagaac tcgccgcgct ggagctttcc gacgagctcg agcaacatct
    16921 tctcgagctg ctcgtgggtc tggccatcga gcgaatcggc gtagagctcg tagtagccgc
    16981 cgtcgcgcag gaacgtcggg ttgcagccgg cgttcgcgac cttctgcgtg atgagcaggt
    17041 cgcgcgcaag aatgccggag cggccatcga ggagcaaaag gtccatcagc tcgagcggga
    17101 ggcgatcggg atgcgtcgcc gacacgagcg tccagccgag gagaacgccc tcatcggacg
    17161 gcaccttgac cgagagctcg gtgcgctttg ccaccggtgc gatcgtgccc ggttctgcgg
    17221 cgggacctgc gggacgcttg aacttgccga actctttctc gaggagcggc aagaccgatt
    17281 catcgacatc gccggcgagc atgatcgcca tgttgccggg cgtgtaatag cgggtgaaga
    17341 accgcgtcat gtcgccgtac gcgggcttct tgaggtgctc gacctcgccg aggacgctgc
    17401 tccagccgta gccgtggttc gggaacaacg acttcatgaa cgcatcgcgc acgcggcgct
    17461 cggggctgtc catgccgcgg ttcttttctt cgtagacggc ctcgagctcc ggccagaaca
    17521 ggcggaacac agcgtcgtca tagcggtccg cttccacgtg cgcccattgt tcgatccggt
    17581 tcttcgggat ctcggtgacg tacacggtcg agtcactgtt cgtgaacgcg ttgagcccgc
    17641 cgatgccgag ctgcgagtac atctggtcga gctcgttcgg aatcgcgaac gctgctgcag
    17701 cctgcgtctc gctgtcgatc tctttgagga tcttgtcggc gtcggcgccg ggcttgcgca
    17761 ggtcgtcgta gagcgccgcg atcttgtcga ggtgcggctt ctccttcgcg tagtcctgcg
    17821 t
//
LOCUS       DATLBZ010000590         3812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3452044, whole genome shotgun sequence.
ACCESSION   DATLBZ010000590 DATLBZ010000000
VERSION     DATLBZ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3452044"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /gene="pgi"
                     /locus_tag="VMZ53_29170"
     CDS             complement(<1..1365)
                     /gene="pgi"
                     /locus_tag="VMZ53_29170"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HUS32621.1"
                     /translation="MSALTSSPAWQALVEHQRELESVHMRELFARDPRRVAKMTREAC
                     GIFVDYSKHRADDNVMAKLWGLAKWAKVEEWRDKMFAGEKINVTENRAVLHVALRNRS
                     NRPILVDGKDVMPDVNAVLAKMRAFTDRIRSGDWRGSSGKSISDIVNIGIGGSDLGPV
                     MVTEALTPYAKRWEESAGPSRGAVRVHFVSNVDATHLAEVVKDLNPESTLFIVASKTF
                     TTQETMTNAKSARSWVLSKLGANADVAKHFVAVSTNAKEVAAFGIDTANMFEFWDWVG
                     GRYSLWSAIGLSIACYIGMDNFEELLAGAHDMDEHFRTTSLEGNLPVTLALLGIWYAN
                     FWGAETHAILPYDQYLHRFAAYFQQGDMESNGKGVDRDGQPIVDYTTGPVIWGEPGTN
                     GQHAFYQLIHQGTRLIPCDFIAPIASHNPSAHHPILLANFFAQTEALMKGKTPDEVRA
                     EMKGN"
     gene            1450..2901
                     /locus_tag="VMZ53_29175"
     CDS             1450..2901
                     /locus_tag="VMZ53_29175"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HUS32622.1"
                     /translation="MFARHWAPRGLAAIPDAELDRLAELRFDYLWLMGVWTTGELGRD
                     IARQLFTSHSPSEIESSPYAIARYEVDPRYGGNDALAELRRRLAKRNIGLIVDFVPNH
                     TARDHDWITSNPDIYIRERGARRDQVGEIAYGRDPYFPPWTDTAQLDHRCVATRRVLI
                     ETLQQIATQADGVRCDMAMLVLSDVFAKTWSHHQIMGADPILYGDDNVAPAPGVLARG
                     EFWAEAIDAVRHDFIWIAEAYWGLEGRLQKLGFDYTYDKALYDHLLHGRPAQIHAELA
                     TPIEYQRRCVRFLENHDEPRLAASLPPERAAAALAIATTAPGMRFVHDGQIEGRRERA
                     TIQLARRPDEATRPEQRAGQTPFIEFTDQVSRGIEAMNESSFIQSVHERLLALPRAGD
                     FALLPATDERLVAYRWDDRNTPLVVVVNYSPDLVEANVTLEMHGLAGRRIELRDVLDG
                     ATYEREGSAALHVVLQPWQAHVFTVTTKGLWNG"
     gene            2894..>3812
                     /locus_tag="VMZ53_29180"
     CDS             2894..>3812
                     /locus_tag="VMZ53_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HUS32623.1"
                     /translation="MARPGEPADHERERLREITDDAHGWRRWGPYVSDRAWGTVREDY
                     SADGNAWAYLTYDAARAKAYRWGEDGIAGICDRYQLLCFAPAFWNERDPHLKERFFGV
                     TPYEGNHGEDVKEYYFHVDNTPTHSYMALLYKYPQREFPYRRLVEENRARNGQGFEFE
                     LMDAGAFDDDRYFDILIEYAKEAEEDIAIRITATNRGPDKAPLHILPTLWFRNTWGWG
                     KDEMPEPVIRAEYEGLVADDIATLHDPHLPTAARLGTRWLYAPPTTSLVTDNETNGDR
                     VYGPGNASRKPHTKDAFHRYLCEGQRNAIK"
BASE COUNT          703 a         1252 c         1197 g          660 t
ORIGIN      
        1 gttgcccttc atctcggcgc gcacttcatc gggcgtcttg cccttcatga gtgcctcggt
       61 ctgcgcgaag aagttcgcga gcaggatcgg gtgatgcgcg ctcgggttat gcgacgcgat
      121 cggcgcgatg aagtcacacg ggatcagccg cgtgccttga tggatcagtt gatagaacgc
      181 gtgctgcccg ttcgtgccgg gctcgcccca gatgacgggt cccgtcgtgt aatcgacgat
      241 cggctggcca tcgcgatcga cgcctttgcc gttgctctcc atgtcgccct gctggaagta
      301 cgcggcgaac cgatgcaggt attgatcgta cggcaagatc gcgtgcgtct cggcgcccca
      361 gaagttggcg taccagatgc cgagcaacgc gagcgtgacc ggtagatttc cctcgaggct
      421 cgtcgtgcgg aagtgctcgt ccatgtcgtg agcgcccgca agcagctctt cgaagttgtc
      481 catgccgatg tagcaggcga tcgacagacc gatcgcgctc cacagcgagt agcggccgcc
      541 gacccaatcc cagaactcga acatgttcgc ggtgtcgatg ccgaacgccg ccacttcctt
      601 cgcattcgtc gagaccgcaa cgaagtgctt cgcgacatcc gcattcgcac cgagcttcga
      661 gagcacccag ctccgcgcgc tcttcgcgtt cgtcatcgtc tcttgcgtcg tgaacgtctt
      721 actcgcgacg atgaacagcg tcgactcggg attgagatcc ttgacgacct cggcgaggtg
      781 cgtcgcatcg acgttcgaga cgaagtgcac gcgcaccgcg ccgcggctcg gcccggccga
      841 ctcttcccag cgcttcgcat aaggcgtcag agcctcggtg accatcaccg gaccgaggtc
      901 cgatccgccg atgcctatgt tcacgatgtc cgagatcgac ttgccgctgc tgccgcgcca
      961 atcacctgat cggattcgat cggtgaacgc gcgcatcttc gcgagcaccg cgttgacgtc
     1021 cggcatcacg tctttgccgt cgacgaggat cggccgattc gagcgattgc gcagcgcgac
     1081 gtgcagcacc gcgcggttct cggtgacgtt gatcttctcg cccgcgaaca tcttgtcgcg
     1141 ccactcttcg accttcgccc acttcgcgag gccccacagc ttggccatca cgttgtcatc
     1201 ggcgcggtgc ttcgagtagt cgacgaagat gccgcacgct tcgcgagtca tcttggcgac
     1261 gcgccgcggg tcgcgtgcga acagctcgcg catgtggaca ctctcgagct cccgctggtg
     1321 ctcgaccagc gcttgccagg ctggggacga agtcaacgct gacatgcacg agagaaaacc
     1381 acgacgccga cgacaggtgt acgctcgacg gccggtttgg tgatgtcaac gcatcctgcc
     1441 ctctacgaag tgttcgctag gcattgggca ccacgcgggc tcgcggcgat tccggatgcg
     1501 gagctcgatc gacttgcaga gctccgcttc gattatctgt ggctgatggg cgtatggacg
     1561 acgggcgagc tcggccgcga catcgctcgc cagctcttca catcgcattc tccgtcggag
     1621 atcgagagct cgccatacgc gatcgcgcgc tacgaagtag atccgcgcta tggcggcaac
     1681 gacgcgctcg cggagctgcg acgccggctc gcgaagcgga acatcggtct gatcgtcgac
     1741 ttcgttccga accacaccgc acgcgatcac gattggatca cgagcaatcc ggacatctat
     1801 atccgcgagc gcggcgcgcg gcgcgaccag gtgggcgaaa tcgcgtacgg cagggacccg
     1861 tacttcccgc cgtggaccga taccgcgcag ctcgatcacc gctgcgtcgc cacgcggcgc
     1921 gtcctgatcg aaacgctgca gcagatcgcg acgcaagccg acggcgtgcg ctgcgacatg
     1981 gcgatgctcg tgctgtcgga cgtcttcgcg aagacgtggt cgcatcatca gatcatgggg
     2041 gccgacccca ttctgtacgg agacgacaac gtcgcgccgg cgcccggcgt cctcgcacgt
     2101 ggcgagttct gggccgaagc gatcgacgcc gtgcgccacg acttcatctg gatcgccgaa
     2161 gcgtactggg gcctcgaagg gcggctccag aagctcggct tcgattacac gtacgacaag
     2221 gcgctctacg atcatctgct gcacggcagg cctgcgcaga tccacgcgga gctcgccacg
     2281 ccgatcgagt atcagcgccg ctgcgtgcgg ttcctcgaga atcatgacga gccacgcctc
     2341 gccgcatcgc tgccgccaga gcgcgcggct gcagcactcg cgatcgcaac gacggcgcct
     2401 ggcatgcggt tcgtccacga cggccagatc gaaggacgcc gcgaacgagc gacgatccag
     2461 ctcgcgcgtc gacccgacga ggccacacgg ccagagcagc gcgcagggca gaccccattc
     2521 atcgagttca ccgatcaggt atcgcgagga atcgaagcga tgaatgaaag ttcattcatt
     2581 caatcggtgc acgagcgatt gctcgctctg ccgcgcgccg gagatttcgc gttacttcct
     2641 gcgaccgatg agcggctcgt cgcgtaccgc tgggatgatc gaaatacgcc gctggtcgtc
     2701 gtggtgaact actcgcccga tctcgtcgag gcgaacgtga cgctcgagat gcacggtctc
     2761 gccgggcgcc gcatcgagct ccgcgatgta ttggacggcg cgacgtacga acgcgaaggg
     2821 agcgcggcgc tgcatgtcgt gttgcaacca tggcaagcgc acgtgttcac ggtgacgacc
     2881 aagggcctgt ggaatggcta ggcccggcga gccggccgat cacgagcgcg agcgtctgcg
     2941 cgaaatcacc gacgacgcgc acggctggcg acgctgggga ccgtacgtct ccgaccgcgc
     3001 gtggggcacc gtgcgcgagg actactccgc cgacggcaac gcgtgggcat acctgacgta
     3061 cgacgccgcg cgtgcgaagg cgtatcgctg gggtgaagac ggcattgcgg gcatctgcga
     3121 tcgctatcaa ttgctctgct tcgcgccggc gttctggaac gagcgggatc cgcacctcaa
     3181 ggagcgattc ttcggcgtca cgccgtacga gggcaaccac ggcgaggacg tgaaggaata
     3241 ctacttccac gtcgacaaca cgccgacgca ctcgtacatg gcgctgctct ataagtaccc
     3301 gcagcgcgag tttccgtatc gccggctcgt cgaggagaac cgcgcgcgca atggccaggg
     3361 cttcgagttc gagctcatgg atgccggcgc gttcgacgac gatcgctact tcgacatctt
     3421 gatcgagtac gcgaaggaag ccgaagaaga catcgcgatt cgcatcaccg cgacgaaccg
     3481 cgggcccgac aaggcgccgc tgcacatcct tccgacgctg tggtttcgca acacctgggg
     3541 ctggggcaaa gacgagatgc ccgagcccgt gatccgcgca gagtacgaag gcctcgtcgc
     3601 cgacgacatc gcgacgctgc acgatccaca ccttccaacc gccgcgcgcc tcggtacgcg
     3661 ttggctgtat gcaccaccca cgacgtcgct cgtcaccgac aacgagacga acggcgatcg
     3721 cgtgtacggc ccgggcaacg catcgcgcaa gccgcacacg aaggacgcgt ttcatcgcta
     3781 cctgtgcgaa ggccagcgca acgcgatcaa ga
//
LOCUS       DATLBZ010000591        10296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7160362, whole genome shotgun sequence.
ACCESSION   DATLBZ010000591 DATLBZ010000000
VERSION     DATLBZ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10296
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7160362"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2026
                     /locus_tag="VMZ53_29185"
     CDS             <1..2026
                     /locus_tag="VMZ53_29185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS32624.1"
                     /translation="SIGDAVIATDATGIVTFMNPVAERLTGWHTAEAQGHTLLEVFPI
                     FNEDTGEVVENPVQKVIREGVIVGLANHTVLRRRDGSEIPIDDSAAPIRITPDAIEGV
                     VLVFRDATEEKREHLRRAFLARASHELIEAVDYRDALQRIASLAVPRLADWASVEVIE
                     PGDSTSRQLAVAHVDPSKVEYARELNLRYPPDPNAPTGVPQVIRTGKSELYPSIPREL
                     LEAGARDAEHLRIIRELDLRSALVVPLRGKQHVFGAITFIYAQSDRHYSEADLEFAEE
                     LARRAALIIERRRLEEEAEQANRMKDEFLATISHELRTPLQAILGYATMLERGVARDQ
                     GKAISAILRNASAQARLIEDILDMSGIQSGKLRIAVGRVQLTAAIREALDALRPAAAA
                     KQIKLVEQLGPNLGEIEGDFDRLQQIVWNLVSNAVKFSEAGGTVEVMAERVGDRMRVT
                     VRDQGKGIAQEHLSTIFERFRQVDSSTTRQHAGLGLGLAIVKYLVEAHGGTVSAQSAG
                     VGKGATFSVELPAHPGPEHGTPGAPVVPIDASQLKGVHILIVDDDDDARELISDALVE
                     MGARVVRASTTAQAFAFLQAEPPNILISDIGMPVEDGYSFIRRVRALPPEKGGETPAI
                     ALTAYARPEDIRKTEEAGFQLHVVKPVRIENLIEAVKSCVRWSPRDTVRG"
     gene            2023..2469
                     /locus_tag="VMZ53_29190"
     CDS             2023..2469
                     /locus_tag="VMZ53_29190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32625.1"
                     /translation="MTATAAQFRDTLKSVHPGKLSLDDAETIVELAQMAVDADGQEDV
                     DEIKTFFALGKAIYELAGMLDAPTPTFIGDDDDLERIRELGAKLATNEARELAYATAH
                     LLTVIDVQIAPEEDAFLEKLRVALGITEDRADELAAKMSAAITPAE"
     gene            complement(2470..3099)
                     /gene="can"
                     /locus_tag="VMZ53_29195"
     CDS             complement(2470..3099)
                     /gene="can"
                     /locus_tag="VMZ53_29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HUS32626.1"
                     /translation="MSLLEEIFAANRRWAADQLKEDPDFFKRLEAIQTPDLLWIGCSD
                     SRMPANEIIGRRPGEVFVHRNVANVVEHTDVNCLSVLQYAVEVLKVKHVVVVGHYGCG
                     GVRAAMQAQPLGLIDNWLRHIRDVHLWNREELNAIEDVNARANRLAELNVESQVANVC
                     HTTIVQDAWRRGQNLTVHGWIYSLSDGLLRDLGLVVESPDQIPPEYRLY"
     gene            3228..3974
                     /locus_tag="VMZ53_29200"
     CDS             3228..3974
                     /locus_tag="VMZ53_29200"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUS32627.1"
                     /translation="MRIFSIIPADENSEAGLPPPAGLIEEMMRLGAESAARGVLLDQG
                     GLQPSKYGARVEFKNGKPRVTDGPFAETKELIAGYAILRVTDLEDGIAEAKKLPLRCE
                     VEIRPIFELGEPLTNVTPRKPGTKRYMLVFKAGAESTEPPSQEIIQAMGAYNDSLTAA
                     GALLAGEGLQPSVAAARVDLSTGEIKRGPFDPKGLVTGWWMIQAKDKSEAIEWAKRVP
                     IPEGVIEVRPVMDPGESCAQGAHDLAAAAS"
     gene            3986..4408
                     /locus_tag="VMZ53_29205"
     CDS             3986..4408
                     /locus_tag="VMZ53_29205"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUS32628.1"
                     /translation="MRFMVMVKANKDSEAGIPPSQELLAAMGKFNEELVRAGVMLAAE
                     GLHPSSKGARVDYKDGKRTVIDGPFAETKELIAGVWILQCSSLQECIEWMKRCPNPHN
                     DDGQIEIRQIFELGDFELNAENQQRFDGLKTQLEQPKA"
     gene            4432..5682
                     /locus_tag="VMZ53_29210"
     CDS             4432..5682
                     /locus_tag="VMZ53_29210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS32629.1"
                     /translation="MQPASQTQATINALWRIESPKLIARLARMTRDVGLAEELAQDAL
                     VTALEKWPESGVPENPGAWLMATAKNRALDTIRRNKMVERKHEELAPPVMERPKIEDA
                     LDDDVGDDMLRLIFTACHPVLSTEARLALTLRLLGGLTVPEIARAFLSPEPTIAQRIV
                     RAKKTLADAKVPFEVPRGDERDERLSSVLEVLYLIFNEGYSATAGDDWMRPALCEDAM
                     RLGRVLAGLQPNEPEVHGLVALMEIQASRAKARTGPNGEAILLLDQDRSRWDWTLIGH
                     GLAALERALALGGNGPYVVQAAIAACHARARRAEDTNWAMIVQLYNALLELVPSPVIE
                     LNRAVAVSMAEGPAAALAVVDELRKEPALESYYLLYGVRADLLIKLGRNAEAIPELER
                     AAALTKNERERAVLRERIRKISTS"
     gene            5738..6004
                     /locus_tag="VMZ53_29215"
     CDS             5738..6004
                     /locus_tag="VMZ53_29215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32630.1"
                     /translation="MGCCPRDKLCDRCLDDALAQLRGVAACRGEYWARSVATRLPRTK
                     PWPAYEGRCASIAREKVEDLGRDTRLREELARLCVHWAARWWAS"
     gene            6020..6820
                     /locus_tag="VMZ53_29220"
     CDS             6020..6820
                     /locus_tag="VMZ53_29220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS32631.1"
                     /translation="MDRNPQAKQMADESMVRNLRAQAEAIWPQEEPIMRGHGTPRDIL
                     DIGCGTGEITSRLARLFTEANVTGVDLIEAHLELARERYAELGERVTFRQADAFELPF
                     GDKSFDLAVCRHMLQAVPTPERVMAEMVRVTKPGGKLHLIVEDYDMIHASPARLDVGS
                     FWHVAPRVFGGATGTDLHIGRNAYAHLRKLPVDDIRIDYAIVDTQRVPRKTFAQIFEA
                     WRDGYAADISRYLGLPPTDVRDHFDATIECIREGYAVWFVPIVTCRVR"
     gene            complement(6821..7081)
                     /locus_tag="VMZ53_29225"
     CDS             complement(6821..7081)
                     /locus_tag="VMZ53_29225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32632.1"
                     /translation="MTTIDLSLLASVTGGEPPAPGAGTPPDSGRPAAGGTDTAAPLTV
                     DTLRQFCAGLDMQSRRARNNDPVVANALSRAADACYSVLPPK"
     gene            7269..9308
                     /locus_tag="VMZ53_29230"
     CDS             7269..9308
                     /locus_tag="VMZ53_29230"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUS32633.1"
                     /translation="MRVSVGLLLIAACGSSSPKPEAPATSDEGVHKVIVVHGRKSGHY
                     DEVKQPDGSLKVTLDILENGRGPHVDAVMRLADDGTIATFDATGHHEMGNKSFEKFSI
                     LDGVATWESGEEHGRADFAQQFFNPLSETPTLQHIVTAALKAGGSVRLLPAGQAHVTQ
                     VAELTVSANGRSKKIVGYAVTGIDFRRAYTWFADDGTWFGSFSSWSSTVDDAWMPVID
                     QVVHEQERLDRELDQQTYTANAQKPPAAGLAFTHARVLDVEKGAFLDDQTVVIVGDSI
                     KAVGPTAKTKVPDGAQVIDLAGQSLIPGMIDMHSHTNKTDAVLDIASGVTTVRDVGND
                     PEQLDAWKDAFDTGKAVGPRIVRMGFIEGRNEKAASSKITAETPEEAKAAVDYYAKRG
                     WEGIKIYNSMKTELVPVLTAEAHKRGMLVTGHVPVHMVANEAVRAGYDGIEHINQVAL
                     NFFATHDTDTRTPLRFTLVGDKAPSLDLASQPVKDFIALLVEHHTVIDPTMIAFQDLY
                     LGKPGEVVRGWDKYAARMPSQVQRYFLVNGLPGAETKRELYVQAWQKLLAMVKTFYEA
                     KVHLVIGTDHIGGLAYDRELELFAEAGIPNAAVLKMATLDAARAMKLDGKLGSIAPGK
                     VADMFVVAGDPVKSIADIANVKMTVSRGVLFSSDPLFQAVGTKPASEPMMGSGKK"
     gene            9295..10146
                     /locus_tag="VMZ53_29235"
     CDS             9295..10146
                     /locus_tag="VMZ53_29235"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUS32634.1"
                     /translation="MARSSDRIAENVDGDFFVDSSCIDCDLCRQLAPGVFARTDRRAQ
                     SFVTAQPRDDREQHRALMALVTCPTSSIGTTRKIDSRAAARSLPEEIGDGIYFCGWAS
                     EDSYGAAAWLIRRPDGNVLVDSPRAASTLLDQIQLLGGVRYMFLTHRDDVADHEAFHE
                     RFGCERIVHSADAVPGAERVIDGVEPVRLADDLVIVPVPGHTRGSCVLLHRGYCFSGD
                     HVWANDDETGLEAGRDVCWYSWPELKKSMARLAEHRFTWVLPGHGRRWRAPTEEAMRA
                     ELLRLSR"
BASE COUNT         1895 a         3239 c         3487 g         1675 t
ORIGIN      
        1 cagcatcggc gacgccgtga tcgcgacaga cgcgaccggc atcgtcacgt tcatgaatcc
       61 cgtcgccgag cggctgaccg gctggcacac cgctgaggcg cagggacaca cgctgctcga
      121 ggtcttcccg atcttcaacg aagacacagg cgaagtcgta gaaaaccccg tccagaaagt
      181 cattcgcgag ggcgtgatcg tcggtctcgc aaatcacacg gtgctgcggc gtcgcgatgg
      241 aagcgagatt ccgatcgacg acagcgctgc gccaatccgc atcacgcctg atgccatcga
      301 aggcgtcgtg ctcgtcttcc gcgatgcaac ggaggagaaa cgcgagcacc tgcgcagggc
      361 gttcctcgca cgcgcgtcgc acgagctcat cgaggcggtg gactatcgcg acgctctgca
      421 gcggatcgcg tctctcgcag tcccgcggct tgccgactgg gcttcggtcg aggtgatcga
      481 gcccggcgat agtacatccc gccagctcgc ggtcgctcac gtcgatccgt cgaaggtcga
      541 atatgcgcgc gagctcaatc tgcgataccc gcccgatcca aacgcgccga ccggcgttcc
      601 acaggtgatt cggaccggca agtcggagct atatccttcg atcccacgcg agctcctcga
      661 ggcaggtgcg cgcgatgccg agcatctccg catcattcgc gagctcgacc tgcgttctgc
      721 gctcgtcgtg ccgctgcgtg gcaagcagca cgtgtttggc gcgatcacgt tcatctatgc
      781 gcagtccgat cgccactact cggaggccga cctcgagttt gcggaggagc tcgcgcgtcg
      841 agccgcgttg atcatcgagc gccggcgcct cgaggaagaa gcagaacaag cgaatcgcat
      901 gaaggacgag ttcctcgcga cgatcagcca cgagctgcgc acgccgctgc aagcgatcct
      961 cggctacgcg acgatgctcg agcgtggcgt cgcgcgcgac caaggcaaag ccatcagcgc
     1021 gatcttgcgc aacgcgagcg cgcaagctcg tctcatcgag gacatcctcg acatgtcggg
     1081 catccagagc ggcaagcttc ggatcgccgt cggccgtgtc cagctcacgg cggcaatccg
     1141 cgaagcactc gacgcgcttc gacccgcggc tgcagcaaag cagatcaagc tcgtcgagca
     1201 actcggccca aacctcggcg agatcgaagg cgacttcgat cgcctgcagc agatcgtgtg
     1261 gaacctcgtc tcgaacgccg tgaagttcag cgaggccggc ggcaccgtcg aggtcatggc
     1321 ggagcgagtc ggcgaccgga tgcgcgtgac cgttcgcgat cagggcaagg gcatcgcgca
     1381 ggaacacctg tcgacgatct tcgagcgctt ccgccaggtc gacagctcga cgacgcggca
     1441 acacgccggc ctcggcctcg gtctcgcgat cgtgaagtac ctcgtcgaag cgcacggtgg
     1501 caccgtcagc gcgcagagcg cgggcgttgg caaaggcgcg acgttctcgg tcgagctgcc
     1561 cgcgcatccg ggccccgagc acggcacgcc gggcgcaccg gtggtcccga tcgatgcctc
     1621 tcagctcaaa ggcgttcaca tcttgatcgt cgatgacgac gacgatgctc gagagctgat
     1681 cagcgacgcg ctcgtcgaga tgggtgctcg cgtggtgcgc gcatcgacga cggctcaagc
     1741 cttcgcgttc ctgcaagccg agccgccgaa catccttatc tcggacatcg gcatgccggt
     1801 cgaggacggc tactcgttca ttcggcgcgt gcgggcgctt cctccggaga aaggtggcga
     1861 gacgcccgcg atcgcgctga ccgcgtatgc gcgccccgag gacatccgga agaccgagga
     1921 agcgggcttt cagctccatg tcgtcaagcc cgtgcgcatc gagaacctga tcgaggccgt
     1981 gaagtcgtgt gtccggtggt ctcctcgcga tactgttcgc ggatgaccgc gaccgccgcg
     2041 cagttccgcg acaccctgaa gagcgtgcat ccggggaagc tgtccctcga tgatgcagaa
     2101 acgatcgtcg agctcgcgca gatggcggtc gacgccgatg gccaagaaga cgtcgacgag
     2161 atcaagacct tcttcgcgct cggcaaggcg atctacgagc tcgccggaat gctggacgcg
     2221 ccgacgccga cattcatcgg cgacgacgat gatctcgagc gcatccgcga gctgggcgcg
     2281 aagctggcga cgaacgaagc acgcgagctc gcgtatgcga cggcgcactt gctgacagtc
     2341 atcgacgtgc agatcgcgcc cgaagaagac gcgttcctcg agaagctgcg cgtcgcgctc
     2401 ggtatcaccg aggatcgcgc cgacgagctc gcggcgaaga tgagcgccgc cattacgccc
     2461 gccgagtaat cagtagagcc ggtattccgg cggaatctga tcgggcgatt cgacaacgag
     2521 gccgaggtcg cgaagcaggc cgtcgctcag cgagtagatc cagccgtgca ccgtgaggtt
     2581 ttggccgcgc cgccaggcgt cctgcacgat cgtcgtgtgg cagacgttcg cgacttgcga
     2641 ttcgacattg agctcggcga ggcgattcgc gcgcgcgttg acatcctcga tcgcgttgag
     2701 ctcttcgcgg ttccacaagt ggacgtcgcg gatgtggcgc agccagttgt cgatgaggcc
     2761 gagtggttgc gcctgcatcg ccgcgcgcac gccgccacag ccgtagtggc cgacgacgac
     2821 gacgtgcttc accttgagca cctcgactgc atactgcagg accgagaggc agttgacgtc
     2881 ggtgtgctcg acgacgtttg cgacgttgcg atgaacgaac acctcgccgg ggcgacggcc
     2941 gatgatctcg ttcgccggca tgcggctgtc ggagcagccg atccagagga ggtccggcgt
     3001 ctggatcgct tcgagccgct tgaagaagtc agggtcttcc ttcagctgat cggcggccca
     3061 gcgccggttc gcggcgaaaa tctcctcgag aaggctcacg gcgggctcga tgtagcacga
     3121 cttgtatcca agtcgctgaa atgccagcgc gaaaaaatct tcgcgagcta tgtcgattcg
     3181 aggagccctg gttcgtcgcc tctctgaaag ccaacaagga gcgacagatg aggatctttt
     3241 cgattattcc cgccgatgag aacagtgaag ccggtctccc gccacccgcg ggcctcatcg
     3301 aagagatgat gcggcttggc gccgagtccg ctgcacgcgg tgtgctgctc gaccaaggtg
     3361 ggctccagcc cagcaagtac ggtgcgcgcg ttgaattcaa gaacggcaag ccgcgcgtca
     3421 cggatggccc gttcgcggag acgaaggagc tgatcgccgg ctacgcgatc ctgcgcgtga
     3481 cggatctcga ggacggcatc gccgaggcga agaaactgcc gctgcgctgc gaggtcgaga
     3541 ttcgcccgat cttcgagctc ggcgagccgc tgacgaacgt tactcctcgc aagccgggca
     3601 cgaagcgcta catgctcgtg ttcaaggccg gcgccgaatc gacggagccg ccgtcgcagg
     3661 agatcatcca ggcgatgggc gcgtacaacg attcgctcac cgcagcgggt gcgctgctcg
     3721 ctggcgaagg tctgcagccg agcgttgcgg cggcgcgcgt cgatctgtcc accggcgaga
     3781 tcaagcgtgg gccgttcgat ccgaaggggc tcgtcaccgg ctggtggatg attcaggcca
     3841 aggacaagtc cgaggccatc gagtgggcga agcgcgtgcc cattcccgag ggcgtgatcg
     3901 aggtgcgtcc ggtgatggat ccgggcgaga gttgcgcgca aggtgctcac gatctcgcgg
     3961 ctgccgcatc gtaaggagaa cagacatgcg tttcatggtg atggtcaaag ccaacaagga
     4021 cagtgaagcc ggcatcccgc caagccagga actgctcgct gcgatgggca agttcaacga
     4081 ggagctcgtg cgtgcgggcg tgatgctcgc cgccgaggga ctgcacccga gctcgaaagg
     4141 cgcgcgcgtc gattacaagg acggcaagcg gacggtgatc gacggtccgt tcgccgagac
     4201 aaaggagctc atcgccggcg tgtggatcct ccagtgcagc tcgctgcagg agtgcatcga
     4261 gtggatgaag cgctgcccga acccgcacaa cgacgacggc cagatcgaga ttcggcaaat
     4321 tttcgagctc ggcgatttcg agctgaatgc ggagaaccag cagcgcttcg atgggctcaa
     4381 gacgcagctc gagcagccga aggcgtagct cctgcgctac gctgcgcggc ggtgcaaccg
     4441 gcatcgcaaa cgcaagcgac catcaatgcg ctgtggcgca tcgagtctcc caagctgatc
     4501 gcacgactcg cgcgcatgac tcgcgatgtt ggcctcgccg aggagctcgc acaggatgcg
     4561 ctcgtgacgg cactcgagaa gtggccggag tcaggtgtgc ccgagaatcc gggcgcatgg
     4621 ctcatggcca ccgcaaagaa tcgcgcgctc gacacgatcc gccgcaacaa gatggtcgag
     4681 cgcaagcacg aagagctggc cccacccgtg atggagcgtc cgaagatcga agatgcgctc
     4741 gacgatgacg tcggcgacga catgcttcgg ctgatcttca cggcgtgcca tccggttctc
     4801 tcgaccgagg cgcggctcgc cttgacgctg cggctgctcg gcggtctgac cgttccggag
     4861 atcgcgcgcg cgttcctctc gccggagccg acgatcgcgc agcggatcgt gcgcgcaaag
     4921 aagacgctgg ccgatgcgaa ggtgcccttc gaggtgccgc gcggtgacga gcgcgacgag
     4981 cggctctcga gtgtgctcga ggtgctgtac ctgatcttca acgagggtta ctcggcgacg
     5041 gcgggcgatg actggatgcg gcccgcgctc tgcgaggatg cgatgcgcct cggccgcgtg
     5101 cttgcgggac tgcagccgaa cgagccggag gtgcacggcc tcgtcgctct gatggagatc
     5161 caggcgtcgc gggcaaaggc gcgcacgggc ccaaacggcg aggcgatcct gctgctcgac
     5221 caagaccgct cgcgctggga ctggacgctg atcggtcacg gcctcgcggc gctcgaacgc
     5281 gcgctcgccc tcggcggcaa cggaccgtac gtggtgcagg cggcgatcgc ggcgtgtcac
     5341 gcgagggcgc ggcgcgccga ggacacgaac tgggcgatga tcgtgcagct gtacaacgcg
     5401 ctgctcgagc tcgtgccgtc tccggtgatc gagctcaatc gcgccgtcgc ggtgtcgatg
     5461 gctgaaggcc cggcggcggc gctcgcggtc gtcgatgagc tgcgcaagga gcccgcgctc
     5521 gagagctact acttactcta cggcgtgcgc gcggatctat tgatcaagct cggccgcaat
     5581 gcagaggcga ttccggagct cgagcgtgca gcggcgctca cgaagaacga gcgggagcgc
     5641 gctgtcttgc gcgaacgcat tcgcaaaatc agtacgtcgt aaacgcgagc aaagaggctc
     5701 cgacgtgcgg cgtcagagct aagcgctacg atcgatcatg ggctgttgtc cgcgcgacaa
     5761 gctctgcgat cgatgtctcg atgatgcgct cgcccagttg cgcggcgttg cggcttgtcg
     5821 tggcgagtat tgggcgcgca gcgtcgcgac tcgattgccg cgcacgaagc cgtggccggc
     5881 gtacgagggc aggtgcgcga gcatcgcacg cgagaaggtc gaggacctcg gccgcgatac
     5941 ccgcttgcgg gaagagctcg ctcggttatg cgtgcactgg gcggcgcgct ggtgggcgag
     6001 ctaatctcgc gagcgcgtta tggatagaaa tccgcaggcc aagcagatgg cggacgagtc
     6061 gatggtccgc aacctccgcg cgcaagccga agcgatctgg ccgcaggagg agccgatcat
     6121 gcgcgggcac ggcacgccgc gcgacatcct cgacattggc tgcggcacgg gtgagatcac
     6181 gtcgcggctc gcgaggctgt tcacggaggc aaacgtgacc ggcgtcgatc tcatcgaggc
     6241 gcacctcgag cttgcgcgcg agcgttacgc agagctcggc gagcgcgtca cgtttcgcca
     6301 agccgatgcg ttcgagctgc cgttcggcga caagtcgttc gacctcgcgg tctgccgcca
     6361 catgttgcaa gccgtgccga cgcccgagcg cgtgatggcc gagatggtgc gagtgaccaa
     6421 gcccggcggc aagctgcacc tcatcgtcga agactacgac atgatccacg cgtcgccggc
     6481 gcgtctcgac gtcggctcgt tctggcatgt ggcgccacgc gtgttcggag gcgcgaccgg
     6541 cacggacctc catatcggcc ggaatgcgta tgcgcatctg cgcaagctgc ccgtcgacga
     6601 catccgcatc gactacgcga tcgtcgatac gcagcgtgtt ccacggaaga cgttcgcgca
     6661 gatcttcgaa gcgtggcgcg acggctacgc ggccgacatc tcgcgctatc tcgggctgcc
     6721 accgaccgac gttcgcgatc atttcgatgc gacgatcgaa tgcatccgcg aaggctacgc
     6781 cgtctggttc gtgccgatcg taacctgtcg cgtgcgctga ttacttcggc ggcaggacgg
     6841 agtagcaagc gtcggcggcg cggctcagcg cattggcgac cactggatcg ttattgcgag
     6901 cgcgccgcga ctgcatgtcg agccccgcgc agaactggcg tagcgtatcc acggtcagcg
     6961 gagccgcggt atcagtaccg ccagcggctg ggcgtccgct atcgggtggc gtaccagcgc
     7021 ctggagcggg tggctcacca ccggtgaccg aagcgaggag cgagaggtcg atcgtcgtca
     7081 tgcagctcga cgcagcatcg tgtgtgccag cgagcacgtg cagcccgcgg gacgcaggcg
     7141 aagcgccgcg atgacgagca atcgcgggta tggcagcgtc tggcgcatgc gcggccagtc
     7201 ctcagatggc aacacgacag gccgccggct gatcgagctt ccgccgccgc ggtgatagtg
     7261 tgcgcgtcat gcgcgtttct gtcggtttac tactcatcgc ggcatgcggc tcgtcgtcgc
     7321 ccaaaccgga ggcgccggcg acaagcgacg aaggtgttca caaggtgatc gtggtgcatg
     7381 gccgcaagtc cggccactac gacgaggtga agcagcccga cggctcgctg aaggtcacgc
     7441 tcgatatcct agagaacgga cgcggccctc acgtcgatgc ggtgatgcgt cttgccgacg
     7501 acggcacgat cgccacattc gacgcgaccg ggcaccacga gatgggcaac aagagcttcg
     7561 agaagttctc gatcctcgat ggggttgcga cgtgggagtc gggcgaagag cacggcaggg
     7621 cggacttcgc gcagcaattc ttcaatccgc tgtcggaaac gccgacgctg cagcacatcg
     7681 tcacggccgc gctcaaggcg ggtggctcgg tgcggctgct tcccgctggg caagcgcacg
     7741 tcacgcaggt ggcggagctg accgtctccg cgaacggacg gagcaagaag atcgtcggct
     7801 acgccgtgac cggcatcgac ttccgccgcg cgtatacgtg gttcgccgac gacggcacgt
     7861 ggtttggttc cttctcttcg tggtcgtcga ccgtcgatga cgcgtggatg cccgtcatcg
     7921 atcaggtcgt tcacgagcaa gagcggctcg atcgcgagct cgaccagcag acgtacaccg
     7981 cgaacgcgca gaagccgccg gcagccgggc tagcgttcac gcacgcgcgg gtgctcgacg
     8041 tcgagaaggg cgccttcctc gatgatcaaa cggtcgtcat cgtcggcgac tcgatcaaag
     8101 ccgtcggccc gaccgccaag acgaaggttc cggacggcgc gcaggtgatc gatctcgccg
     8161 gccagagcct catcccgggc atgatcgaca tgcactcgca cacgaataag accgacgcgg
     8221 tgctcgatat tgcatccggg gtcacgaccg ttcgcgacgt cggcaacgac cccgagcagc
     8281 tcgacgcatg gaaggacgcg ttcgacaccg gcaaggctgt tgggccccga atcgttcgca
     8341 tgggctttat cgagggacgt aacgagaagg ctgcgtcgag caagatcacg gcagagacac
     8401 cggaagaggc gaaggcggcg gtcgactact acgcgaagcg tgggtgggag gggatcaaga
     8461 tctacaactc gatgaagacc gagctcgtgc cggtcctcac ggcggaggcg cacaagcgcg
     8521 ggatgctcgt gacgggtcac gttccggtcc acatggtggc gaacgaggcg gtgcgcgccg
     8581 gctacgacgg catcgagcac atcaatcagg ttgcgctgaa cttcttcgcg acgcacgaca
     8641 ccgacacgcg cacgccgctg cggttcacgc tcgtcggcga caaagcgccg tcgctcgatc
     8701 tcgcaagcca gccggtgaag gacttcatcg cgctcctcgt cgagcaccac accgtcatcg
     8761 atccgacgat gatcgcgttc caagatttgt atctcggcaa gccaggcgaa gtcgttcgcg
     8821 gttgggacaa gtacgccgcg cggatgccgt cacaagtgca gcggtacttc ctcgtcaatg
     8881 gattgccggg cgccgagacc aagcgcgagc tctacgtgca ggcatggcag aagctgctcg
     8941 caatggtgaa gacgttctac gaagcgaagg tccacctcgt gatcggcacc gatcacatcg
     9001 gcggccttgc ctacgatcgc gagctcgagc tgttcgccga ggccgggatc ccgaacgcgg
     9061 ccgtgctgaa gatggcgacg ctcgacgcgg cgcgcgcgat gaagctcgac ggcaagctcg
     9121 gctcgatcgc gccggggaaa gtcgcagaca tgttcgtcgt cgcgggcgat ccggtgaagt
     9181 cgatcgcgga catcgcgaac gtgaagatga cggtgagccg cggcgtgttg ttcagctcgg
     9241 acccgctctt tcaggcagtt ggcacaaagc cggcttctga gcccatgatg gggagtggca
     9301 agaagtagcg atcgcatcgc cgagaacgtc gacggagact tcttcgtcga tagctcgtgc
     9361 atcgattgcg atctctgccg ccagctcgcg ccgggcgtgt tcgcgcgaac ggatcgccgc
     9421 gcacagtcgt tcgtgaccgc gcagccgcgc gatgatcgcg agcagcaccg cgcactgatg
     9481 gcgctggtca cgtgcccgac gagctcgatc ggcacgacgc gcaagatcga cagccgcgct
     9541 gcggcgcgct cgctgcccga agagatcggc gatgggatct acttttgcgg ctgggcatcc
     9601 gaggacagct acggagcagc ggcgtggctg atccggcggc cggacggcaa tgtgctcgtc
     9661 gattcaccgc gtgcagcgag cacactcctg gatcagatcc agctactcgg cggcgtgcgc
     9721 tacatgtttc tcacgcaccg cgacgatgtc gccgatcacg aggcgttcca cgagcggttc
     9781 ggctgcgagc gcatcgtgca ttccgcggac gccgtgccag gtgcggagcg agtgatcgac
     9841 ggtgtcgaac cggtgcggct cgccgatgat ctcgtcatcg tcccggtgcc aggccacacc
     9901 cgtggcagct gcgtgctgct gcatcgcgga tactgcttct ccggtgacca cgtctgggcg
     9961 aacgacgacg agaccgggct cgaagcgggg cgagacgtct gctggtactc gtggccggag
    10021 ctcaagaagt cgatggcgcg gctcgccgag catcgcttca cgtgggtgct tcccggccac
    10081 ggccgtcgct ggcgcgctcc gacggaagag gcgatgcgtg ccgagctcct gcggctctcg
    10141 cgctagcggg gacggagtcc gttacgccgg acgcagaatg aggcctgcgt tgctcgcggc
    10201 gaagaagtcc ttcaccttgc ggacgttgtc ctcgaacgtc acgaggcggc tcgacggacg
    10261 ctggacgcgg agccagacaa gccgggtcga gccggt
//
LOCUS       DATLBZ010000592         6757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7160835, whole genome shotgun sequence.
ACCESSION   DATLBZ010000592 DATLBZ010000000
VERSION     DATLBZ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7160835"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VMZ53_29240"
     CDS             complement(<1..1074)
                     /locus_tag="VMZ53_29240"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HUS32635.1"
                     /translation="MADVFIYDALRTPRGRGKKGALNHIHPQELLAQTLNHMAERKKL
                     DKSLVEDVSMGCVTQVKEQGACIARLALIAAQWPDDVTGFTINRFCGSGLEAVNSIAA
                     KIGAGFIEAGIGGGVESMSRVPMGSDEAMLDGLNENLRKRLYLVPQGISADLIATREG
                     FTREDVDKFALASQQKAARAIEAKAFANSLFTVKNLDGTPALDRDEHPRADSTLEALG
                     GLKPAFVQMGAMAVGPNGETLDQLALQRYPDTKQINHVHTGGNSSGIVDGAATVLMGS
                     AEWGKKAGLKPRARIRSMATAGAEPVIMLTAPAPASEKALKKAGMTPKDVDLWEINEA
                     FAVVPLQTARKLNIDLDRVNVNGG"
     gene            complement(1109..1720)
                     /locus_tag="VMZ53_29245"
     CDS             complement(1109..1720)
                     /locus_tag="VMZ53_29245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32636.1"
                     /translation="MRWPLLLIVVAACNSHDQQAARPVEPQGVPLTNTTTRTCKEAAL
                     GLEGATRGVRDPGNAVAGPLTLRCHEDAWPVAALECFATMREGELGKCGRLLSEASRS
                     KLFGVLGGYGDAHMGIAIAHARLEQLEVGVKACDDFVGAVTAVLACERVPLDVRVQLG
                     QETADFWALPTSKLAVEDVQRMSLVCGESLASLVQQATDAGCK"
     gene            complement(1823..2935)
                     /locus_tag="VMZ53_29250"
     CDS             complement(1823..2935)
                     /locus_tag="VMZ53_29250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32637.1"
                     /translation="MALVPSLLMAGCTGSDPSSEDPDPGADDPGQEQPADDFAEDSPP
                     VDDGYVPRAFTATRFGVFYQVSSDVLDIYQAADTGLPQAADHAWIITQSHATAFASKT
                     LADRIHQRTDFYYAPAFDLWDASHAGWQTATDAQLRQWSHDFRDAALKAHADLFTFNE
                     APTTTGASANVRTKISKILRFIHEPDAKGRVLWGVTYLTERAATASRYDSAASDFFKA
                     IDETSIALVAEHYHSTGFVCNQSEAALAEHYFNFRSWLVASGEPAKLSIAAKKYTVLH
                     SSRFNEGMSGWAGGDSTQTTLANYQRALSKITRVTRMTEGGVNRLSFAPTTTQITMVG
                     VQPRITALFRWHYRHTAAQSTELPCIDNFGGNCSCQ"
     gene            complement(3074..4180)
                     /locus_tag="VMZ53_29255"
     CDS             complement(3074..4180)
                     /locus_tag="VMZ53_29255"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HUS32638.1"
                     /translation="MTRPGIDRWLQDQPAVLVANPTAHSGKAADTIRHARALLDKAKI
                     VHRFIPTEPEGATIERVREQIDDHGARVVIYMGGDGTFAEVAKGILRSGHAKQVAMGM
                     LPNGTANDQGKSFGLDAGAWALARNVMVIAEGYTVGCDVAHLVIERGLKEVHRDMFFD
                     SFSVGLGAASLGTRNRDREIVGRIPGLGMIYRDQLVYLGAVARRFFESYVVDTKFDLD
                     CVIDGKVHQWPDLLDVIVKNTKIFGGEWIFDPQSESDDGRVELIPVKGRRDFTAKLLG
                     TMRRVPIGMDDLEKLGMEHDPAIPGAHMELTVRAEGGILPAAQVDGEEIPAGDRYVID
                     VAARALRLVVPREHVDPSATDSLSQPIAMPSDEE"
     gene            4212..5330
                     /locus_tag="VMZ53_29260"
     CDS             4212..5330
                     /locus_tag="VMZ53_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS32639.1"
                     /translation="MVSEEHELIRKTVRQFAEKRVEPQAMEHDESGTFNVGLLRELGQ
                     MGLIGITIPEEDGGAGLDAVASCIVHEELSYSDPGFALGYLAHALLFVNNFYWAGNAA
                     QRKKYLPKVLSGEHVGAMGMTEPAVGTDVLGMQTVARREGDGYVINGRKIFITNGPEC
                     DVVIVYAKLDDRITTFIVERDFAGFTKSPKIPKLGMRASTMSELMFDNCRVPAENLLG
                     TEGGGITNMMRNLEIERLGLSAMSLGLARRCLDVMVRYSQERKTFGKPIHEHGQIQRY
                     IGDSYAKIEAMKALIYNVADTVGPTKRNRLGTDAAKLFASTAAKEVADNAIQVLGGYG
                     YCTEYRVEQLWRDAKLIEIGGGTLEAHQKNITRDLVRG"
     gene            5453..>6757
                     /locus_tag="VMZ53_29265"
     CDS             5453..>6757
                     /locus_tag="VMZ53_29265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32640.1"
                     /translation="MGQYPHDEPTVTLSDRAPERPSATALAPSTMLGRYVVLSELARG
                     GMSVVYVAYDPELDRRVALKVVRRSKLSEIHRARLHREAQALARLSHPSVVTVFDVGD
                     IEDDTFVAMELLDGVTLREWVKQKPRTWREVVRVIVAAGRGLAAAHEAGIVHRDVKPD
                     NIVIAASGAVKLVDFGIARDLGDRSADSGEMAQVEAAALEAAARDSVDDLQLAANSSG
                     GLAARPLEQITQLGFIVGTPAYMPPEQRSQKPEADERSDQYSLCATLYEALYKQKPLT
                     STKKAILDRREALTVADRPGDTRSLAAPAPKGSDVPAWLQRVVTRGLAVNPAQRYPSV
                     DALLRELDRDPARALRRGVAAVGALAAVAALAIVVTAKLMPSHAPGPSCGTGEDRVAG
                     VWNANRRADLERVGATRGGPIAGAALGMFATTVDQYVAGWQGM"
BASE COUNT         1147 a         2123 c         2281 g         1206 t
ORIGIN      
        1 gccgccgttc acgttcacgc gatcgagatc gatgttgagc ttgcgcgcgg tctgcagcgg
       61 cacgactgcg aacgcctcgt tgatctccca gagatcgacg tctttgggcg tcatgccggc
      121 tttcttcagc gccttctcgg aggcgggggc gggggccgtc aacatgatga ccggctcggc
      181 gcctgcggtt gccatcgagc gaatgcgtgc acgcggcttg aggccggctt tcttgcccca
      241 ctccgccgaa cccatgagga ccgtggcggc accgtcgacg atgccggacg agttgccacc
      301 cgtgtggacg tgattgatct gcttcgtgtc ggggtagcgc tggagcgcga gctggtcgag
      361 tgtctcgccg ttcgggccga ccgccattgc gcccatctgg acgaacgccg gcttgagacc
      421 gccgagggct tcgagcgtcg agtcggcgcg cgggtgctcg tcgcgatcga gagcgggcgt
      481 gccgtcgagg ttcttgaccg tgaagaggga gtttgcgaac gctttcgctt cgatcgcgcg
      541 cgctgccttc tgttgcgacg cgagggcgaa cttgtcgacg tcttcgcgcg tgaagccttc
      601 acgcgtcgcg atgaggtcgg cgctgatgcc ctgcggaacg aggtacaggc gcttgcgtag
      661 gttctcgttc agaccgtcga gcatggcttc gtcgctgccc atcggcacgc gcgacatcga
      721 ctcgacaccg ccgccgatgc cggcttcgat gaagcctgcg ccgatcttgg ctgcgatcga
      781 gttgacggcc tcgaggcccg agccgcagaa gcgattgatc gtgaagccgg tgacgtcgtc
      841 aggccattgc gcagcgatca gggcgagacg ggcgatgcac gcgccttgct ctttgacctg
      901 cgtgacgcag cccatcgaga cgtcctcgac gagcgacttg tcgagcttct tgcgctccgc
      961 catgtggttg agcgtttgcg cgagcagctc ctgcggatgg atgtgattga gtgcgccctt
     1021 cttgccgcga ccacgtgggg tgcggagagc gtcgtaaatg aagacgtcag ccatggcact
     1081 gcctccttag atgcgaggca atctagcatt acttgcagcc ggcgtcggtc gcctgctgca
     1141 cgagcgaagc tagcgattct ccacagacta gcgacatgcg ctgcacgtct tcgaccgcga
     1201 gcttgctcgt gggaagcgcc cagaagtctg cggtttcttg gccgagctgc acgcgaacgt
     1261 cgagcgggac gcgctcgcac gcgaggacgg ctgtgaccgc gcccacgaag tcgtcgcacg
     1321 ctttgacgcc gacctcgagc tgctcgagcc gcgcgtgcgc gatcgcgatg cccatgtgtg
     1381 cgtcgccgta accgccgagg acgccgaaca gcttgctgcg cgacgcttcg gagagcaggc
     1441 ggccgcactt gccgagctcg ccttcgcgca tcgtcgcgaa gcactcgagt gcggcgaccg
     1501 gccacgcgtc ttcgtgacag cgcagcgtga gcggaccggc gacggcattg cctggatcgc
     1561 gaacgccacg cgtcgcgcct tcgagtccga gcgcggcttc tttgcacgtg cgcgtcgtcg
     1621 tgttcgtgag cgggacgcct tgcggctcga cgggacgagc ggcttgctga tcgtgcgagt
     1681 tacacgcggc gacgacaatc agcagaagcg gccaacgcat tttttcagcg tagcaggcac
     1741 tcgaaaaagg cgcccgcgcg cgtgcacgta ctcggatgat gatgagggta aacgacgagt
     1801 acgtacacgc acacgggctg aactattggc agctgcagtt gcctccgaag ttgtcgatgc
     1861 acggcagttc ggtgctctgc gccgcggtgt gccggtaatg ccatctaaag agagcggtaa
     1921 tgcgcggttg aacgccgacc atcgtgatct gcgtcgtcgt cggtgcgaat gagaggcgat
     1981 tgacgccgcc ttccgtcatg cgcgtgactc gcgtgatctt cgagagcgcg cgttggtagt
     2041 tcgcgagcgt cgtctgcgtc gagtcgccgc ccgcccagcc actcatgcct tcgttgaagc
     2101 gcgacgagtg caggaccgtg tacttcttcg ctgcgatgga gagctttgca ggctcgccgc
     2161 tcgcgacaag ccacgatcgg aagttgaagt agtgctcggc cagcgctgct tcgctctgat
     2221 tacagacgaa gccggtcgag tgatagtgct ctgcaacgag cgcgatgctg gtctcgtcga
     2281 tcgctttgaa gaagtcggaa gcggcggagt cgtagcggct cgccgtggca gcgcgctccg
     2341 tcagatacgt gacgccccac agcacgcgac ccttcgcatc gggctcgtga atgaagcgga
     2401 ggatcttcga gatcttcgtg cggacgttcg cagacgcgcc cgtcgtggtc ggcgcttcgt
     2461 tgaacgtgaa gaggtcggcg tgcgctttga gtgcggcatc gcggaagtcg tgcgaccact
     2521 gccggagctg cgcgtcggtg gccgtttgcc agcccgcgtg cgacgcatcc cagagatcga
     2581 acgcgggtgc gtaatagaag tcggtgcgct gatgaatacg atcggcgagc gtcttgctcg
     2641 cgaacgcggt cgcgtgactc tgcgtgatga tccacgcgtg atccgcggct tgcggcaggc
     2701 ccgtgtcggc agcttgatag atgtcgagga cgtcgctcga gacttggtag aagacgccga
     2761 agcgcgtcgc ggtgaatgcg cgtggaacgt agccgtcgtc gacgggcgga ctgtcctcgg
     2821 cgaagtcgtc ggcgggttgc tcttgccctg gatcgtctgc gcccggatcc ggatcttcgg
     2881 agctcggatc gctgccagtg caacctgcca tgagcaggga tggcactagc gccatgactc
     2941 ggagcgtccg catcgtgccg gcgctatcag cacgatgtgt gccgcgcgaa cgcgcttcgc
     3001 aaacccgcgc acctacacgg cggcacatcg acgcggtgag gacgctgcac aacaagtgca
     3061 aacggcggtg tgctcactcc tcgtcggaag gcatcgcgat cggctggctg aggctgtccg
     3121 tcgccgacgg atcgacatgc tcgcgcggca ccacgagacg cagcgcgcgt gcggcgacat
     3181 cgatcacgta gcgatcgccg gccgggattt cttcgccgtc gacctgcgct gccggaagga
     3241 tgccgccttc ggcgcgaacc gtgagctcca tatgagcgcc gggaatcgcg gggtcgtgct
     3301 ccatgccgag cttctcgaga tcgtccatgc cgatcggaac gcggcgcatc gtgccgagca
     3361 gcttcgcggt gaagtcgcga cggcctttga cgggaatcag ctcgacgcgg ccgtcgtcgg
     3421 attcgctctg cggatcgaag atccactcgc cgccgaagat cttcgtgttc ttgacgatca
     3481 cgtcgagcag atcgggccac tgatggacct tgccgtcgat cacgcaatcg agatcgaact
     3541 tcgtgtcgac gacgtagctc tcgaagaagc ggcgcgcgac cgcaccgaga tagacgagct
     3601 gatcgcgata gatcatgccg aggccgggaa tgcgtccgac gatctcgcga tcgcgattgc
     3661 gcgtgccgag actcgcggcg ccgaggccga ccgagaacga atcgaagaac atgtcgcgat
     3721 gaacttcttt gagaccgcgc tcgatcacga gatgcgcgac gtcgcagccg acggtatagc
     3781 cctctgcgat caccatgacg ttgcgcgcga gtgcccacgc gccagcgtcg aggccgaacg
     3841 acttgccttg atcgttcgcc gtaccattcg gcagcatgcc cattgcgact tgcttcgcgt
     3901 gaccggagcg taggatccct ttggcgacct cggcgaacgt gccgtcgcca cccatataga
     3961 tgacgacgcg cgcgccgtga tcgtcgatct gctcgcggac gcgctcgatc gtcgcgcctt
     4021 caggctcggt cgggatgaag cgatggacga tcttggcttt gtcgagcaga gcgcgcgcgt
     4081 ggcgaatcgt gtcggcggcc ttgcccgaat gcgcggtcgg atttgccacg agcacggcgg
     4141 gctggtcctg caaccatctg tcgattccgg gccgcgtcat ctagctctct gaatggtacg
     4201 gtacgctccg catggtgtca gaagagcacg agctgattcg caagaccgtg cggcagttcg
     4261 ccgagaagcg tgtcgaaccg caggcgatgg agcacgacga gtccgggacg ttcaacgtcg
     4321 gccttctccg cgagctcggc cagatggggc tcatcggcat cacgatcccc gaagaagatg
     4381 gcggcgccgg gctcgatgcg gtcgcgtcgt gcatcgtcca cgaagagctg tcgtacagcg
     4441 atccgggttt cgcgcttggc tacctcgcac acgcgcttct gttcgtgaac aacttctact
     4501 gggccggcaa tgcggcgcag cgcaagaagt acctgccgaa ggtgctgagc ggcgagcacg
     4561 tcggcgcgat gggcatgacg gagcccgcgg ttggcaccga tgtcctcggc atgcagacgg
     4621 tcgcgcgccg cgaaggtgat ggctacgtca tcaacggccg caagatcttc atcacgaacg
     4681 gtcccgagtg cgatgtcgtg atcgtgtacg cgaagctcga cgatcgcatc acgacgttca
     4741 tcgtcgagcg cgacttcgcg ggcttcacga agagcccgaa gattcccaag ctcggcatgc
     4801 gcgcgtcgac gatgagcgag ctcatgttcg acaactgccg cgtgcccgcg gagaatcttc
     4861 tcggcaccga aggcggcggc atcaccaaca tgatgcgcaa cctcgagatc gagcgcctcg
     4921 gtctctccgc gatgagcctc ggcctcgcgc gccgctgcct cgatgtgatg gtgcgctact
     4981 cgcaagagcg caagacgttc ggcaaaccga tccacgagca cggccagatc cagcgctaca
     5041 tcggcgattc gtacgcgaag atcgaggcga tgaaggcgct gatctacaac gtcgccgaca
     5101 ccgtgggacc gacgaagcgc aaccgcctcg gcaccgatgc ggcgaagctg ttcgcgtcga
     5161 cagctgccaa agaagtcgcg gacaacgcga tccaagtcct cggcggctac ggctactgca
     5221 cggagtaccg agtcgagcaa ctgtggcgcg acgccaagct catcgagatc ggcggcggca
     5281 ccctcgaggc gcatcagaaa aacattacgc gcgacctcgt tcgcggctag tggcgtgaca
     5341 gtcgcgtaag cgaccccacc gacctgacat ccgaacgttt cggagcctgg atgtcggtga
     5401 tcgacgacgg tccgttccgt gtatcgtgac gtggggcttt tgcgcctcgt ctatggggca
     5461 gtatccacac gacgagccga ccgtcacgtt gagcgaccgc gcgccggagc ggccgtcggc
     5521 gactgcgctc gcgccgagca cgatgctcgg gcgttatgtc gtgctgtccg agctcgcgcg
     5581 cggcggcatg agcgtcgtgt acgtcgcgta cgaccccgag ctcgatcgtc gcgtcgcgct
     5641 caaagtcgtg cggcgctcga agctgtcgga gatccatcgc gctcgcttac atcgcgaagc
     5701 gcaggcgctc gctcggctct cgcatccgtc cgtcgtcacg gtgttcgacg tcggcgatat
     5761 cgaggacgac acgttcgtcg cgatggagct gctcgacggc gtcacgctgc gcgagtgggt
     5821 gaagcagaag ccgcggacat ggcgtgaggt ggtgcgcgtg atcgtcgcgg cgggtcgcgg
     5881 tctcgcagca gcacacgaag caggcatcgt tcatcgcgat gtgaagcccg acaacatcgt
     5941 gatcgctgca tcgggcgccg tgaagctcgt cgacttcgga atcgcacgtg atctcggcga
     6001 ccgcagcgca gactctggcg agatggcgca ggtcgaggcg gcggcgctcg aggccgcagc
     6061 gcgcgacagc gtcgacgatc tgcagctcgc cgccaatagc agtggcggtc ttgcagcgcg
     6121 cccactcgag cagatcacgc agctcggctt catcgtcggt acgccggcgt acatgccgcc
     6181 cgagcagcgc agccagaagc ccgaagcgga cgagcgctcc gatcaataca gcttgtgcgc
     6241 gacgctctac gaggcgctct acaagcagaa gccgctcacg tcgacgaaga aggcgatcct
     6301 cgatcgccgc gaggcgctca ccgtcgctga tcggccgggt gacactcgct cgctcgcggc
     6361 gccggcgccc aaaggcagcg acgtgccggc gtggctgcag cgcgtcgtca cgcgcggact
     6421 cgcggtgaat ccagcgcagc gctatccgtc ggtcgatgcg ctgctccgcg agctcgatcg
     6481 cgatcccgcg cgtgcattga ggcgcggcgt cgcagcagtc ggggctctcg cggccgtcgc
     6541 cgcgctcgcg atcgtcgtca ccgcgaagct gatgccatcg cacgcgccgg gtccgtcgtg
     6601 cggcaccggc gaggatcgtg tcgcgggcgt gtggaatgcg aatcgccgcg cagatctcga
     6661 gcgcgtaggc gcgacgcgcg gcggtccgat cgctggcgct gctctcggca tgttcgcgac
     6721 gacggtcgat caatacgtcg ccggttggca gggcatg
//
LOCUS       DATLBZ010000593         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7864052, whole genome shotgun sequence.
ACCESSION   DATLBZ010000593 DATLBZ010000000
VERSION     DATLBZ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7864052"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..658
                     /locus_tag="VMZ53_29270"
     CDS             83..658
                     /locus_tag="VMZ53_29270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32641.1"
                     /translation="MRGMSLALLVVTASIGNAFADEQATTETNETSGTGGGAPGDTPA
                     TGESRPADKGTLGVGLVIGEPTGISAKLYLEDDQAIQGAIGPAFIGGGIALHGEYVLH
                     PWILQDRDSFVLPVYLGPGVRFVDYYGGRSTGTSSSHFAVGALGVAGMLFDFKDVPLD
                     VFVELGGGAEYDFTNGWGPVLNAGAGVRYYP"
     gene            659..1522
                     /locus_tag="VMZ53_29275"
     CDS             659..1522
                     /locus_tag="VMZ53_29275"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS32642.1"
                     /translation="MSKPDELEARTADGVTLRLDRVAARGERRGVVVCLHAMMTDGRY
                     FGARRDTGFAQLLADAGLDVYVADFRGHGRSVPPHAGKDDWSFDDLVEHDLPTILTTV
                     AVTSQVPTSSVAIVGHSLGGLVTCAALGTKRIEPPRVLGLAATSVWLGDTVQRRAIMT
                     AYRGVTALFGKAPIRALRAGTADESATYVKQLTGWSSSGRWTSLAGVDYMVALETITT
                     PVFAFTGAGDWMCKPDDANAIAKRIRSCEPLRVVGKKHGDTLDPDHFTLFTRPELTTL
                     RREIADRLVAS"
     gene            1546..>2183
                     /locus_tag="VMZ53_29280"
     CDS             1546..>2183
                     /locus_tag="VMZ53_29280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32643.1"
                     /translation="MGAAKSMKKKGGKPETRPAKAKLKPKAGAETKDAKSAAKDDAST
                     RGDRSKRLLDLVMILLRARTPVTYREIRESFPAYQTLNIEAGLRAFERDKADLLDLGV
                     PIRYVTPDEDDSLEDGGYVIDLKRFKMPEVRLTPDEISALVLAASVAHAMPGGTYPKI
                     VDLALKKLAFDLPELPDTPTEFPRPAHNAPTVLVHFPERQNRLASSAVARPEL"
BASE COUNT          365 a          729 c          727 g          362 t
ORIGIN      
        1 ggtccccatg ggtggggatc gaccccgcac ttcgcggccc agcgaatttg cgctcacctg
       61 ccccggcagg tagcgtcgcg ccatgcgtgg gatgtctctg gccctgctcg tcgtgaccgc
      121 gtccatcggc aacgcctttg ccgacgagca ggcaacgacc gagaccaatg agacgagcgg
      181 aacgggaggc ggcgcgcccg gcgacactcc cgcgaccggc gaatcgcgtc ccgccgacaa
      241 gggcacgctc ggcgttggcc tcgtcatcgg cgagccaacc ggcatcagcg cgaagcttta
      301 tctcgaagac gatcaggcga tccagggcgc catcgggccc gcattcatcg gtggcggcat
      361 cgcgctgcac ggcgagtacg tgctccaccc gtggatcctc caagatcgcg attcgttcgt
      421 gttgcccgtg tacctcgggc ccggcgtgcg gttcgtcgat tactacggcg gtcgcagcac
      481 cggcactagc agctcgcact tcgcggtcgg cgcgctcggc gtagccggca tgctgttcga
      541 tttcaaagac gtcccactcg atgtgttcgt cgagctcggc ggtggcgcag agtacgactt
      601 cacgaacggc tggggtccag tgctcaacgc gggcgctgga gtgcggtact acccgtaggt
      661 gagcaagccc gacgagctcg aggcgcggac cgccgacggt gtcacgctgc ggctcgatcg
      721 cgttgcggca cgtggcgagc gacgtggcgt ggtcgtctgc ctgcacgcga tgatgacgga
      781 cggtcgctac ttcggcgcgc gtcgtgacac cggcttcgcg cagttgctcg cggatgcggg
      841 cctcgacgtc tacgtcgcag actttcgcgg ccacggtcgc tcggtgccac cgcacgcggg
      901 caaagacgac tggagcttcg acgatctcgt cgagcacgat ctgccgacga tcctcacgac
      961 cgtcgctgtc acgtcgcagg ttccgacctc gagcgtcgcg atcgtgggtc actccctcgg
     1021 cggcctcgtc acgtgcgccg cactcggcac gaagcgcatc gagccaccgc gcgtgctcgg
     1081 gctcgcggcg accagcgtgt ggctcggcga taccgtgcag cgtcgcgcga tcatgacggc
     1141 gtatcgaggc gtcaccgcgc tgttcggcaa ggcgccgatt cgcgcgctgc gtgcgggcac
     1201 cgccgacgaa tcggcgacgt acgtcaaaca gctcaccggt tggtcgagta gcggccggtg
     1261 gacgagcctt gccggcgtcg attacatggt cgcgctcgaa acgatcacga cgccggtgtt
     1321 tgcattcacc ggagccggcg attggatgtg caagcccgat gacgcaaacg cgatcgcgaa
     1381 gcgaatccgc agctgcgagc ctctgcgcgt cgttggcaag aagcacggcg acacgctcga
     1441 tcccgatcac ttcacgctgt tcacgcgtcc ggagctgaca actctccgtc gagaaatcgc
     1501 ggatcgactc gttgcttcgt gacggttgtg ttaagaaccg tggccgtggg ggctgccaag
     1561 tccatgaaga agaagggcgg gaagccggag acgcgtccgg cgaaggcgaa gctcaagccg
     1621 aaggccggcg ctgagacgaa ggatgccaag tccgccgcga aggacgacgc cagcacgcgc
     1681 ggcgatcgca gcaagcgcct gctcgatctc gtgatgatcc tgctgcgcgc ccgtacgccc
     1741 gtcacgtatc gcgagatccg cgaatcgttc cccgcgtatc agacgctcaa catcgaagcg
     1801 ggcctgcgcg cgttcgagcg cgacaaagcg gacctgctcg atctcggcgt gccgattcgc
     1861 tacgtgacgc ccgacgagga cgactcgctc gaagacggcg gctacgtgat cgatctcaag
     1921 cgtttcaaga tgccggaagt gcggctgacg cccgacgaga tttccgcgct cgtgctcgca
     1981 gcgtcggtcg cgcatgcgat gcccggcggc acgtatccca agatcgtcga cctcgcgctc
     2041 aagaagctcg cgttcgatct gccggagctg cctgacacgc cgacggagtt tccgcgtccc
     2101 gcgcacaacg cgccgacggt gctcgttcac ttccccgagc ggcagaatcg tcttgcgagc
     2161 tcggcggtcg cacgcccgga gct
//
LOCUS       DATLBZ010000594        11878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3461917, whole genome shotgun sequence.
ACCESSION   DATLBZ010000594 DATLBZ010000000
VERSION     DATLBZ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461917"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="VMZ53_29285"
     CDS             complement(<1..1605)
                     /locus_tag="VMZ53_29285"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HUS32644.1"
                     /translation="MRIRRFAAMGIVAASACMATYATTEQHVVIVDVSTYNFGTVAVG
                     QSSTSSTITISPQTQTDDDNVTAIAFQSACPNFALNLSQPLPAHIYCDGSAGMARTAA
                     GSGTQSCIPHVYAFTATFTPSFAGTQSCNVRIDYAPTGSGSGSGGSTFVMLTGTGMAT
                     ANSWTVSPLQLLYGDVPVSQPSSPQPVTIMNTGTQALTIMGTVSNTSVFGVGGAPLSS
                     HSLAAGASETYQVTCTPNSATMFNATLTFTGTNVATQTVALSCRGITTTLNVSPNPAI
                     FAYTLVGRSPADRTVTINNGGSASVTISNFRVGAGASTDLTITSSPGTTTLLNGGSAQ
                     VVLHYAATKPNLGSLGTIVFDTGSATSQNIAVSGDAQVGSIGTNPASIELGPVCVGAT
                     MSKQLAIFANAPGKVDVSSIGAPASTQIAVPTVAGTLQGNHANELTVTATLTATTPAP
                     IDDKLLINSNLPAEPMIDVPIHGVVLPAGIGASPELVHFGPVMLDTTTTAKEIVISNC
                     SPSPLTVSAASIGGVDAREFAIVSPAD"
     gene            complement(1618..3300)
                     /locus_tag="VMZ53_29290"
     CDS             complement(1618..3300)
                     /locus_tag="VMZ53_29290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32645.1"
                     /translation="MNTRRLLCASVLVGACASQGDPGDKVAGEWAPGKGDGAFELVEA
                     GPAAIDSKIEIALDHRVPAYRVESYGGTKLSIDVAGGDAYVIVEGPLAGDGDKVAIGG
                     GTVAGEDDDSGPGRDAHLDITLDKAGVYRVLTGTYESLGEGGAAEGSVALTVKCSAQC
                     FRPMVDNKTFVHALQAQGGPALTEYAKAEIASLVHDPVVAERMAAQLQTILADPNLTG
                     LDRFPTIPLSAIGALRPALGAIPAEEPKPDQVVTGDLMAFLGACSPDRSLPADVDQRL
                     PGVKYGQFPSKTLSPCQYAHASKLGQVLTSLAANNGSSVTFKGQTIKTPKELFAALIA
                     AGHTIEVRNERMYANFLSLIAGDRDVIWPVWIDTGIRLSNGESLSIPVGHSHHAWRIS
                     GPTINTRVMFYLGVSGAGFFGQTDQRPAWSGTIASTTSTQADVLATVDAATSYLRRNR
                     IERTTVAQGKPADGYGYVGVCNDSNATIEHVTKGTITTFPLLRAKELDNAASLNDGLD
                     ATVRALPKDGDGISDSRDALRRAVAMQPFPAGTSLWDAKLTSQLAKARADLQ"
     gene            3436..5337
                     /locus_tag="VMZ53_29295"
     CDS             3436..5337
                     /locus_tag="VMZ53_29295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32646.1"
                     /translation="MGRPIDQPKTEATRLGSYEIVRKLARGGMAELFLARTIGPEGFE
                     KLVVLKKILPTHAENPKFVRLFLDEAKLVATLDHPHIAHVYDMGKVDGNYFFTMEYVH
                     GQDVRSTLRRTSRLDKKFPTDHAVHIARNIAAALHYAHERKRPDGGLLDIVHRDVSPS
                     NIIISYDGAVKLVDFGVAKAATSTVKTRTGTLKGKISYMSPEQAKGSPIDRRSDIFSL
                     GIVLWEMATTQRLFKSDNDLATIQMIINSQAEPPSKHNPEIPAELDRIVMKALANKLE
                     DRYQTAEQMQLDLEEYAREQKLKQSTVALRSYMHELFAAEISAWTVAQAKGETLTDYL
                     LSPPGENLTTPVSESELDFISDDEDEDDEENVDSEQATALPSVSGIATRQISVPPPPR
                     MSEGILRVVESTPAPSIVVADQTPPPVRIEANGARAADQSFPIAPREWRHSAPIETMS
                     GISGEKTKQMLIGASLVFGAIVLIAVMFGGNPPSAASKVAEPPQVDPKVDDPTPVEKQ
                     IDMPQTMGSNTAAGSGAPVEEPAEAGSATDTEMPEEPVEKPATKPTTKPTTTTKPTTT
                     TKPTTTTKPTTTTKPSTTPTTTKPGTTTKPGTTTKPGTTTKPGTTTKPGTPTRPKPRG
                     NGDDPLPPT"
     gene            5422..6102
                     /locus_tag="VMZ53_29300"
     CDS             5422..6102
                     /locus_tag="VMZ53_29300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32647.1"
                     /translation="MLWPALAVADEGQAQPDISKDPAVNEHFHIGLDAYAKQDYSTAS
                     REFELAYQLDPVPTLLFTWAQSERLAGRCAKALPLYRKYLYADINEKQKSATREKIQI
                     YEKAAPPPPPTKQPEVSQTEGPRWYQDKLGGGLTVAGIIGIAVGITYFVKASGSRDDA
                     NKAMFLDDFKAGLDEATTQRRVGAVTLTLGLGLVASGIAVYVIHDKHNKKVVAGTDGR
                     TIFVGARF"
     gene            6102..7346
                     /locus_tag="VMZ53_29305"
     CDS             6102..7346
                     /locus_tag="VMZ53_29305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32648.1"
                     /translation="MRAAVLAVALLACKYDGEFPCTQTDECRTADNAGNCEQAGWCSL
                     HDDTCDSGRRFDETAGDGLAKKCVAGPQGCEDWHPHFFKPCGLPAPSGNLTFNGYYKY
                     DTTDGTLTDLVTMATIPQPSVVIDGVRFLSVQDVTASSASSIALVGNKPLVIAAWGSM
                     VINGLIDASSQGDAGPGANPNGGCNAPTLGADATATTPGGGGGGAALQGNGGNGGGGA
                     AGATKESMPMTIRGGCRGGNSGAAGPGAVSPATADTHARGGMGGGALVLSSRLSLTID
                     GSVTAGGEGGGGSPMGAGCGGAGGGAGGMIVLEGSAIELHGTVFATGGGGGGVATKSG
                     QGMKGRDGSLTGAAGGAAPTGCTGAGGAGSTAATLDGGNGGTGACGGAGGGGGSGWIL
                     VFTQSLDMVGAMTAPMPSTNPF"
     gene            7361..8905
                     /locus_tag="VMZ53_29310"
     CDS             7361..8905
                     /locus_tag="VMZ53_29310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32649.1"
                     /translation="MRLLLLAAVLCACKATGTFKCMTSDQCRLGDEVGYCEDSGYCTF
                     IDDTCPSQRRYDDTAEYPLTNECLTGYVTGRIVEQYIVTDDTGARTLVPLVPSPLTLA
                     AELEDGSVPVVRIDHDGAFSFPAPEGMPYELTNPVTSPLQTDSRHLALSSVIPGRPDV
                     APITKPTPIKFTFNGTSMGPIWISSSGVATETHTTVNGTNFQFDWRDAEPQQGAKLGL
                     LDASKHDELYVTQTMTAVGYVTLSTFAKLQPTLSDGITTDITGMLTPLPRLDCAHIQI
                     AVAAEQMRMKTAKPSVTIQNTTWQLQAATARDTTWIATYLVADSTGAMATPELDVTYA
                     NPFPSTSMLALQYVEGSVTASIAGLPVTLSVTSQTYVPFESVTGTCSATVAIAGTTAF
                     AGEITVAGTRVDTDAKDVTIDLTKRVPVGWTPIVSGPADAWFVSIYEGADDGLGALVF
                     TYVHTIETTETHVTIPANMLKSGHHYVVNIAALQGLPNAATGDFMTVKYPVGATSTWS
                     RSFKVK"
     gene            8902..11154
                     /locus_tag="VMZ53_29315"
     CDS             8902..11154
                     /locus_tag="VMZ53_29315"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32650.1"
                     /translation="MSNQDDPTVANTPRGTAKRALTEPPQPDRYTIVKLLGEGGMGRV
                     YRAHDTRLGRDVAIKMIDVGLQGPDKTQQRERFVREAKAAARLVHPNIAVVHDVDAEA
                     GWLVMELVEGEALREVLAKGPLEAPLVRKVAEQVLAALDAAHGAGIIHRDVKPSNIVL
                     QPNGNVKLVDFGVARLIDMEVTRTGENVGTPAYMAPEQIRGGAIDGRTDLYALGATLY
                     ELVTGARMIAFESPGPQAQDKIEKACGSDRALAQVIVRCLQSDPEARFASARDALTVL
                     EPARPKKRALWPLAMVAFAALAGGGVFALAKMRMPKQKAADARLAKAFTLAQRGENEK
                     ASGLLAEVLADDPLDPDAVTYKLLVDWWEGGILGDVERRVEKVKLSKPQRAMITGIDL
                     ITKRRETEAIAFLEGADRESPNSVEILYALGEARWHGQQLQPGVDTLTRAFMLDPRWQ
                     VALHHVTEFRLSRGEATQLQPIAEKLRVVDPPAGATLDCKIAVGNRDYKGAADIAAKA
                     LERLEKIPELYICLAQAYVLERHFDLAEQTAKAAFDLWPIDMREWGGFAQYAELTLYR
                     GQLDEYLALLRGKPSRQRAIALLLWRPKEETNETQPSGPGMRMPPLGAAVWVLQELTR
                     GRNPVAVYGDYVEPEVAAYGKALRAELQGDLVQATAEYRKALAVPAKGDMRMLLAHHL
                     ARVLAKTGHEADAKQLCEEEVIAPRMYLPYRAVVLPDCLLWAGRGSELLDQWRGFEHP
                     AVVKARAAGK"
     gene            complement(11210..>11878)
                     /locus_tag="VMZ53_29320"
     CDS             complement(11210..>11878)
                     /locus_tag="VMZ53_29320"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HUS32651.1"
                     /translation="FVYSLQMADAFIRTGMYKRILMIGAELHSHSLDYSTRGRDVMVL
                     FGDGAGAMVLGPMETDDPKQGVMYTSVHSDGSGAMDLYLKIYEINRMPYLNYDPSDRE
                     QNALMMYPKMDGKRVFLNAVRGMVMSTNDALAKTGMTWNDITWFVPHQANLRINEKVA
                     EVAKIPPEKVLNTIEFYGNTTAATVPLTIDYWRKQGKVKKGDRILSSVFGSGFTWGAA
                     IFTV"
BASE COUNT         2183 a         3805 c         3946 g         1944 t
ORIGIN      
        1 atccgccggg ctgacgatcg cgaactccct ggcatcgacc ccgccgatgc tcgcagcgct
       61 gaccgtcagc gggctcggcg agcaattcga gatcacgatc tccttcgcgg tcgtcgtcgt
      121 atcgagcatc acaggcccga agtgaacgag ctctggtgac gcaccaatgc cggcgggcag
      181 aacgacgccg tggatcggca catcgatcat cggctccgcc ggcaggttcg agttgatcag
      241 gagcttgtcg tcgatcggcg ccggcgtcgt tgccgtcagt gtggcggtca ccgtcagctc
      301 gtttgcgtga ttgccttgca gcgttccggc caccgtcggc actgcgatct gcgtgctcgc
      361 aggcgctccg atcgacgaca cgtcgacctt gcccggcgcg ttcgcaaata tcgcgagctg
      421 cttgctcatc gttgcgccca cgcatacagg gccgagctcg atgctcgccg ggttcgtgcc
      481 gatcgagcca acttgggcat cgcccgacac cgcgatgttc tgcgatgttg ccgaccccgt
      541 atcgaacacg atcgtgccga gcgagccgag gttcggcttc gtcgctgcgt agtggagcac
      601 gacctgggcc gagccaccgt tgagcagcgt cgtggtacca ggcgaagacg taatcgtcag
      661 gtccgtcgac gcgccggcgc cgaccctgaa gttgctaatc gtgacgctcg cgctgccacc
      721 gttgttgatc gtcacggttc gatccgcggg cgagcgaccg acgagcgtat acgcgaagat
      781 ggcgggattc ggcgaaacgt tgagcgtcgt cgtgattccg cgacagctca gcgccaccgt
      841 ctgcgtcgcc acattcgtgc ccgtgaacgt gagcgtcgcg ttgaacatcg tcgcgctgtt
      901 cggcgtgcac gtcacttgat acgtctcgga ggcaccagcg gcgagcgagt gcgacgacag
      961 cggcgcgcca ccgacgccga acaccgacgt gttgctgacg gttcccatga tggtcagcgc
     1021 ctgcgtgccc gtattcatga tcgtgaccgg ctgcggcgag ctcggctgcg agacaggcac
     1081 atcgccgtag aggagctgca gcggcgatac cgtccacgaa ttcgcggtcg ccattccggt
     1141 gccggtgagc atcacgaacg tcgagccacc gctgcccgat ccagatccgg tcggcgcgta
     1201 atcgatgcgg acgttgcacg actgcgtgcc cgcaaacgac ggcgtgaacg tcgccgtgaa
     1261 tgcgtacacg tgcgggatgc agctctgcgt gccgctgccc gctgccgtgc gcgccatgcc
     1321 ggcgctgcca tcacaataga tgtgcgcggg aagcggctgg ctgaggttca gcgcgaagtt
     1381 cgggcacgcc gattggaatg cgatcgccgt aacgttgtcg tcgtcggtct gcgtctgcgg
     1441 cgagatcgtg atcgtcgagc tcgtcgagct ctggccgacc gccacggtgc cgaagttgta
     1501 ggtcgagaca tcgacgatga cgacgtgttg ctcggtcgtc gcgtaggtcg ccatgcaggc
     1561 acttgccgcg acgataccca tcgcggcgaa acggcgaatc ctcacggagc gaggatatca
     1621 ctgcaggtcg gcgcgtgcct tcgcgagctg ggaggtgagc tttgcatccc acagtgaagt
     1681 acccgccgga aacggctgca tcgccaccgc gcgacggagt gcgtcgcgag aatcactgat
     1741 gccgtcgcca tctttcggca gcgcgcgaac cgtcgcatcg aggccgtcgt tcagcgaagc
     1801 cgcgttgtcg agctccttcg cacgcagcag cgggaacgtc gtgatggtgc ccttggtcac
     1861 gtgctcgatc gtcgcgttcg agtcgttgca tacgccgacg taaccgtagc catcggccgg
     1921 cttgccctgc gcgaccgtcg tgcgctcgat gcgattgcgg cgcagatacg atgtcgcggc
     1981 atccaccgtc gcgaggacgt ccgcctgcgt gctcgtcgtc gacgcgatcg tgcccgacca
     2041 cgccggacgc tgatccgtct ggccgaagaa gccggcgccc gagacgccga gatagaacat
     2101 cacgcgcgtg ttgatcgtcg ggccgctgat ccgccacgcg tggtggctgt gcccgacggg
     2161 aatcgagagg ctctctccgt tcgagaggcg gatgcccgtg tcgatccaca ccggccagat
     2221 gacgtcgcga tcacccgcga tgagcgagag gaagttggcg tacatgcgct cgttgcgaac
     2281 ttcgatcgtg tgacccgccg cgatgagcgc cgcgaagagc tccttcggcg tcttgatcgt
     2341 ttgacccttg aacgtcaccg acgagccgtt gtttgcggcg agcgacgtca gcacctggcc
     2401 gagcttgctc gcatgcgcgt actgacacgg cgagagcgtc ttgctcggga actgcccgta
     2461 cttcacgcct ggcagccgct gatcgacatc tgcgggcaac gagcgatccg gcgagcacgc
     2521 gccgagaaac gccatgagat ctcccgtcac gacttgatcg ggcttcggct cctcggcggg
     2581 aatcgcaccg agcgcaggcc gcagcgcgcc gatcgcggag agcgggatcg tgggaaaccg
     2641 atcgaggccc gtcaggttcg gatccgcgag gatcgtctgc agctgcgccg ccatccgttc
     2701 ggcgacgacg ggatcgtgca cgagcgacgc aatctccgcc ttcgcgtact cggtgagcgc
     2761 cggaccgcct tgcgcttgca gcgcgtggac gaacgtcttg ttgtcgacca tcggacggaa
     2821 gcactgcgca ctgcacttca cggtgagagc aacgctgccc tccgccgccc cgccttcgcc
     2881 aaggctctcg tacgtgccag tgagaacgcg atagacgccg gccttgtcga gcgtgatgtc
     2941 gagatgcgca tcgcgaccgg ggccgctgtc gtcgtcttcg cctgcaacgg tgccgccgcc
     3001 gatcgcgacc ttgtcgccat caccagcgag cggaccttcg acgatcacgt atgcgtcgcc
     3061 gcccgcgaca tcgatcgaca gcttcgtgcc gccgtacgac tcgacgcggt acgcaggcac
     3121 gcgatggtcg agcgcgattt cgattttgct gtcgatcgcc gccgggccgg cctcgacgag
     3181 ctcgaacgcg ccgtcgcctt tgccgggcgc ccactcaccc gccactttgt cgccggggtc
     3241 gccctgtgaa gcgcatgcac cgacgaggac tgacgcgcag agtagccggc gggtgttcat
     3301 gcacacatgt gtttgcacct gttgtgccgg gaaatgcaca agcgttcacg ccagcttgcc
     3361 cacatgtggc aacacgatcc gccgccttta gaacgagcca cgcaaagcgt cattcgttat
     3421 gatctcggcg agggaatggg aaggcccatt gatcaaccga agacggaggc aacccgtctc
     3481 ggctcatacg agatcgtccg caagctcgca cggggcggca tggcggagct gttcctcgct
     3541 cgcacgatcg ggccggaggg attcgagaag ctcgtcgtgc tcaagaagat cctcccgact
     3601 cacgcggaga atccgaagtt cgttcgcctc ttcctcgatg aagcaaagct cgtcgcgacg
     3661 ctcgatcacc cgcacatcgc tcacgtctac gacatgggca aggtcgacgg gaattacttc
     3721 ttcacgatgg agtacgtcca cggtcaggac gtccgctcca cgctgcgccg cacatcgcgc
     3781 ctcgacaaaa agtttccgac cgatcacgcg gttcacatcg cgcgcaacat cgctgcggcg
     3841 ctccactacg cgcatgaacg caagcgtccg gacggcggct tgctcgacat cgtgcatcgc
     3901 gacgtgtcgc catcgaacat catcatctcg tacgacggag cggtgaagct cgtcgatttc
     3961 ggcgtcgcga aggcagcgac gagcaccgtg aagacgcgga ccgggacgct caagggaaag
     4021 atctcgtaca tgtcgcccga gcaggcgaag ggctcgccca tcgatcgccg cagcgacatc
     4081 ttctcgctcg gcatcgtgct gtgggagatg gccacgacgc agcgcctctt caagagcgac
     4141 aacgacctcg cgacgatcca gatgatcatc aactcgcagg ccgagccgcc gtcgaagcac
     4201 aacccggaga ttccggccga gctcgatcgc atcgtcatga aggcgctcgc gaacaagctc
     4261 gaggatcggt atcagaccgc ggagcagatg cagctcgatc tcgaggagta cgcgcgcgag
     4321 cagaagctca agcagtcgac cgtcgcgctg cgctcgtaca tgcacgagct gttcgccgcc
     4381 gagatcagcg cgtggaccgt cgcgcaggcg aagggcgaga cgctgactga ctatctgctg
     4441 tcgcctcccg gcgagaacct gacaacaccc gtcagcgaga gcgagctcga cttcatcagt
     4501 gacgacgagg acgaggacga cgaggagaac gtcgactccg agcaagcaac tgcgctgccg
     4561 tcggtgtcgg gaatcgcgac gcgacagatc tcggtgccgc cgccgccgcg catgtccgag
     4621 gggatccttc gcgtcgtcga gtcgactcca gcaccctcga tcgtggtcgc ggaccaaacg
     4681 ccgccgccgg tacggatcga agccaacgga gcgcgggccg cggatcaatc gttcccgatc
     4741 gcaccgaggg agtggcggca ctccgcgccg atcgagacga tgtccgggat cagcggcgag
     4801 aagacgaagc agatgctgat cggcgcgagc ctcgtgttcg gcgcgatcgt cctcatcgcg
     4861 gtgatgttcg gcggcaatcc gccgagcgct gcgagcaaag tcgcggaacc gccgcaggtc
     4921 gatccgaagg tggacgatcc aacgcctgtc gagaagcaga tcgacatgcc gcagacgatg
     4981 ggcagcaata cggcagcggg ctccggcgcg cccgtcgagg agcccgcgga agcaggctct
     5041 gcgaccgaca ccgagatgcc ggaggagccg gtcgagaagc ccgcgacgaa gccgacgacg
     5101 aaaccgacga ccacgacgaa gccgacgacc acgacgaagc cgacaaccac gacgaagccg
     5161 acgacaacga cgaagccatc gacgactcca acgacgacga agcccggcac gacgaccaag
     5221 cccggcacga cgaccaagcc cggcaccacg accaagcccg gcaccacgac gaaacctggc
     5281 acgccgacga ggccaaagcc gcgcggcaac ggcgacgacc cacttccccc cacctgaaac
     5341 actccgtccg cacgacacgg ctgcgctgat cggagtacga tcacatcgag agcgtcgatg
     5401 ggtcgttttg ctcttctcct tgtgctgtgg cctgcgcttg ctgtcgccga tgagggacag
     5461 gcgcaaccgg atatctccaa ggatccggca gtcaacgagc attttcacat cggcctcgac
     5521 gcgtacgcga agcaggatta ctccacggcg agccgcgagt tcgagctcgc atatcagctc
     5581 gacccggtcc cgacgctgct gttcacgtgg gcgcagtccg agcgactcgc gggccgatgc
     5641 gcgaaagcgc tgccgctgta ccgcaagtat ctctacgccg acatcaacga gaagcagaag
     5701 agcgcgacgc gcgagaagat ccagatctac gagaaggccg cgccgccacc gccgccgacg
     5761 aagcagcccg aggtttcgca aacagaaggg ccgcgttggt accaggacaa gctcggcggc
     5821 gggcttacgg tcgccggcat catcggcatc gccgtcggca tcacgtactt cgtcaaggcg
     5881 tcgggctcgc gagatgacgc gaacaaggcg atgttcctcg acgatttcaa ggccggcctc
     5941 gatgaggcga cgacgcaacg gcgtgttggt gccgtgacgc tgacgctcgg ccttggtctc
     6001 gtcgcgagcg gcatcgcggt ctatgtcatt cacgacaagc acaacaagaa ggtcgtcgca
     6061 ggaacggacg gccgcacgat cttcgtcggg gcgcgcttct gatgcgcgcg gcggttctcg
     6121 ccgtcgcact ccttgcctgc aagtacgacg gagagtttcc gtgcacgcag accgacgagt
     6181 gccgcaccgc agataacgcc ggcaactgcg agcaagcggg ctggtgcagc ctgcacgacg
     6241 acacgtgcga ctctggtcgc cgcttcgacg agaccgccgg cgatgggctc gcgaagaaat
     6301 gcgtcgctgg accgcaaggc tgcgaggact ggcatccgca tttcttcaag ccgtgtggtc
     6361 tcccagcgcc gtcgggaaac ctcacgttca acggctatta caaatacgac acgaccgatg
     6421 gaacgctgac ggatctcgtc acgatggcca cgattccgca gccgagcgtc gtgatcgatg
     6481 gcgttcgctt tttatcggtg caagacgtaa cggcgagctc cgcatcgtcg atcgcgctcg
     6541 tcggtaacaa gccactcgtg atcgctgcat ggggctcgat ggtgatcaac gggctcatcg
     6601 atgcgagcag ccaaggcgat gcaggaccgg gcgcgaatcc caatggcgga tgcaacgctc
     6661 caacgctcgg tgccgacgcc actgcaacga cgcccggtgg cggcggaggc ggcgcagcat
     6721 tgcaaggcaa cggtggcaac ggcggcggcg gtgccgcggg cgcgacgaag gagagcatgc
     6781 cgatgacgat ccgcggtggc tgtcgcggtg ggaacagtgg cgctgccggc cctggtgccg
     6841 tgtcgccggc aaccgcggac acgcacgcgc gaggcggaat gggtggcggc gcgctcgtgc
     6901 tcagctcacg cctgtctctc acgatcgatg gctccgtaac ggccggcggc gaaggcggtg
     6961 gcggctcacc gatgggcgcc ggatgtggcg gtgctggcgg cggcgctggc ggcatgatcg
     7021 ttctggaagg ctcggccatc gagctgcacg gcaccgtgtt tgcgacgggc ggcggcggcg
     7081 gtggcgttgc gacgaagagc ggccaaggca tgaagggtcg tgacggatcg ctcacaggag
     7141 ccgccggcgg cgcagcaccg acgggctgta ccggcgctgg tggcgcgggc agtacggctg
     7201 caactctcga cggtggcaat ggcggaactg gcgcatgtgg cggtgctgga ggtggtggtg
     7261 ggagtgggtg gattctggtg ttcacgcaga gcctcgacat ggtgggtgcg atgaccgctc
     7321 caatgccgag cacgaatccg ttctgagcta ccctgatcag gtgagactgc tcctgctcgc
     7381 cgccgtgctc tgcgcgtgca aggcgacagg aacattcaag tgcatgacgt cggaccagtg
     7441 ccggctcggt gacgaggtcg gttactgcga agactccggc tactgcacgt tcatcgacga
     7501 cacgtgtccg tcacagcgtc ggtacgacga caccgctgag tatccgctca cgaacgagtg
     7561 cctgactggc tacgtgaccg gccgcatcgt cgagcagtac atcgtgaccg acgacaccgg
     7621 agcgcggacc ctcgtcccgc tcgtgccgtc gccgttgacg ctcgctgcag agctcgagga
     7681 tggatcggtg cccgtcgtcc ggatcgatca tgacggcgcc ttctcgtttc ccgcgccgga
     7741 aggaatgccg tacgagctga cgaatccggt tacgagcccg ctccaaacgg attcgcgcca
     7801 tctcgcgctg tcgtcggtca ttcccggccg gcccgacgtc gcaccgatca cgaagccgac
     7861 gccgatcaag ttcaccttca atgggacttc gatgggaccg atctggatca gctcgagcgg
     7921 agtcgcgacg gagacccaca caaccgtcaa cggaacgaac ttccagttcg actggcgcga
     7981 tgccgagcca cagcaaggag cgaagctcgg gcttctcgat gcgagcaagc acgacgagct
     8041 ctacgtcacg caaacgatga ctgcggtcgg ctacgtgacg ctctcgacgt tcgcgaagct
     8101 gcagccgacg ctcagcgacg gcatcacgac ggacatcacc ggcatgctga cgccgctgcc
     8161 gcgtctcgac tgcgcgcaca tacagattgc agtggccgcc gagcagatgc gcatgaagac
     8221 ggcgaagcca tccgtgacga tccaaaacac gacgtggcag ctgcaagcgg cgaccgctcg
     8281 agacacgacc tggattgcca cttacctcgt ggccgacagc acgggcgcga tggcaacgcc
     8341 agagctcgac gtcacctatg ccaacccgtt cccgagcacg agcatgctgg cgctgcagta
     8401 cgtcgaggga agcgtgaccg cgtccatcgc tggtttgccc gtgacgctgt cggtgacatc
     8461 gcagacgtac gtgccattcg agtccgtcac gggcacgtgc tcggcaaccg tcgcgatcgc
     8521 cggaacgacc gcgttcgccg gtgagatcac cgtcgctggg acgcgggtgg acaccgacgc
     8581 caaggacgtg acgatcgatc tgacgaagcg cgtgcctgtg ggttggacgc cgatcgtctc
     8641 cggtcctgcc gacgcgtggt tcgtgtcgat ctacgaaggc gcggacgacg gcctcggcgc
     8701 gctcgtcttc acgtatgtgc acaccatcga gacgacggag acgcacgtca cgattcccgc
     8761 gaacatgctg aagtcggggc atcactacgt cgtcaacatc gccgcgctgc agggtctgcc
     8821 gaatgccgcg accggcgatt tcatgacggt caaatatcca gtcggcgcca cgtcgacgtg
     8881 gtctcgctcg ttcaaggtca agtgagcaac caggacgatc ccaccgtcgc gaacacgccg
     8941 cgcggcaccg cgaagcgcgc gctcacggag ccgccgcagc cggatcgcta cacgatcgtc
     9001 aagctcctcg gtgaaggtgg gatgggccgc gtctatcgcg cgcacgatac gaggctcggt
     9061 cgcgacgtcg cgatcaagat gatcgatgtc gggttgcaag gcccagacaa gacgcagcag
     9121 cgcgagcggt tcgtgcgaga ggcgaaggcc gccgcgcgtc tcgtgcatcc gaacatcgcg
     9181 gtcgtgcacg acgtcgacgc ggaagcgggc tggctcgtga tggagctcgt cgagggagaa
     9241 gcgctgcgcg aggtgctcgc gaaggggccg ctcgaagcgc cgctcgtgcg gaaggtcgcc
     9301 gagcaagtgc tcgcagcgct cgatgctgca cacggggccg gcatcatcca tcgcgatgtg
     9361 aagccgtcga acatcgtgct gcagccgaat gggaatgtga agctcgtcga tttcggcgtg
     9421 gcgcggctca tcgacatgga ggtcacgcgg acgggagaga acgtcgggac gcccgcgtat
     9481 atggcgcccg agcagatccg cggcggagcg atcgatgggc gcacagatct gtatgcgctt
     9541 ggcgcgacgc tctacgagct cgtcacgggc gcacgcatga tcgcgttcga atcaccgggg
     9601 ccgcaggcgc aggacaagat cgagaaggcg tgtggcagcg accgcgcgct cgcgcaggtg
     9661 atcgtccgct gcttgcagtc ggatccggaa gcgcggtttg cgtcggcgcg cgacgcgctc
     9721 acggtgctcg agccggcgcg gcccaagaag cgcgcgctgt ggccgctggc gatggtcgcg
     9781 tttgcggcgc tcgccggtgg cggcgtgttc gcgctcgcga agatgcggat gccgaagcaa
     9841 aaggccgccg atgctcgtct cgcgaaggcg ttcacgctcg cgcagcgcgg tgagaacgag
     9901 aaggcgtcgg gtctgctcgc ggaggtgctc gccgacgatc cgctcgatcc agatgcggtc
     9961 acgtacaagc tgctcgtcga ttggtgggag ggtggcatcc tcggtgacgt cgagcgtcgc
    10021 gtcgagaagg tgaagctcag caagccgcag cgcgcgatga tcacgggcat cgatctgatc
    10081 acgaagcgac gcgagaccga ggcgatcgcg tttctcgaag gtgcggaccg cgagtcgccg
    10141 aattcggtcg agatcctcta tgcgctcggc gaagcgcgct ggcacggcca gcagttgcag
    10201 cccggtgtcg acacgctgac gcgcgcgttc atgctcgatc cgcgctggca agtcgcgctg
    10261 catcacgtca cggagtttcg gctgtcacgc ggcgaagcca cgcagctgca gccgatcgcc
    10321 gagaagctgc gcgttgtcga tccgccggcg ggcgcgacgc tcgactgcaa gattgccgtc
    10381 ggcaaccggg actacaaagg tgcggcggac atcgcggcga aggcgctcga acggctcgag
    10441 aagattccag agctctacat ctgtctcgcg caggcgtacg tgctcgagcg gcatttcgat
    10501 ctcgccgagc agacggcgaa ggccgcgttc gatctgtggc cgatcgacat gcgcgagtgg
    10561 ggtgggttcg cgcaatacgc ggagctcacg ctctatcgcg gccagctcga cgagtacctc
    10621 gcgttgcttc gcggcaagcc gagccggcag cgtgcgatcg cgctgctgtt gtggcgaccg
    10681 aaagaggaga cgaacgagac gcagccgtcc ggcccgggca tgcgcatgcc gccgctcggc
    10741 gccgcggtgt gggtgctgca agagctgacg cgcgggcgaa atcccgtcgc tgtgtatggt
    10801 gactacgtcg agccggaggt cgctgcatat ggcaaggcgc tgcgtgccga gctgcaaggc
    10861 gacctcgtgc aggcgaccgc cgagtatcgg aaggcgctcg ccgtcccggc gaagggcgac
    10921 atgcgcatgt tgctcgcgca tcacctcgcg cgcgtgctcg cgaagacggg acacgaggcc
    10981 gacgcgaagc agctgtgcga ggaggaagtc atcgcgccgc ggatgtacct gccctatcgc
    11041 gcggtcgtgt tgcccgactg cttactgtgg gcaggccgcg gctcggagct cttggatcag
    11101 tggcgcggct tcgagcatcc cgccgtcgtc aaagcgcgcg cggctggtaa atgagcaagg
    11161 tccagcgcgc tggaccgcgc agcagcgccg aaggcgatgc gcagaatcgt tagacggtga
    11221 agatcgcggc gccccaggtg aagccggagc caaagaccga cgacagaatc cgatcgccct
    11281 tcttcacttt gccctgcttg cgccagtagt cgatcgtgag cggcaccgtc gctgcggtcg
    11341 tgttcccgta gaactcgatc gtgttgagga ccttctcggg aggaatcttc gcgacctcgg
    11401 cgaccttctc gttgatgcgc aggttcgcct gatgcggcac gaaccaggtg atgtcgttcc
    11461 aggtcatgcc ggtcttcgcg agcgcatcgt tcgtcgacat caccatgccg cgcacggcgt
    11521 tcaagaagac gcgcttgccg tccatcttcg gatacatcat gagcgcgttc tgctcgcgat
    11581 cgctcggatc gtagttcaga tacggcatgc gattgatctc gtagatcttg agatagagat
    11641 ccatcgcgcc gctgccgtcg gagtgaaccg acgtgtacat gacgccctgc ttgggatcgt
    11701 cggtctccat cggaccgagg accatcgcgc ccgcgccatc gccgaagagc accatcacgt
    11761 cgcggccccg cgtcgaataa tcgagcgagt ggctatgcag ctccgcgccg atcatgagga
    11821 tgcgcttgta catgccggtg cggatgaacg cgtcggccat ctgcaggctg tacacgaa
//
LOCUS       DATLBZ010000595         2984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7174825, whole genome shotgun sequence.
ACCESSION   DATLBZ010000595 DATLBZ010000000
VERSION     DATLBZ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7174825"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VMZ53_29325"
     CDS             complement(<1..1035)
                     /locus_tag="VMZ53_29325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32652.1"
                     /translation="MIGETIGNFKIVSRLGRGGMGEVWLAEQTSIGTKVAIKLLNAAV
                     SEDTEHVQRFFNEARAVSRIQHAGIVKIFDVGHHGGHAYLVMEFLDGESLAQRIAQAK
                     RLPLADLCEIGRQVASVLDATHSAGITHRDLKPDNIFVVPDRELARGYRVKILDFGIA
                     KLTGTLAVVSPRTTGTMGTPAYMAPEQWGDASKVDWRADLYSLGCVTFEMATGRPPFI
                     VSNIAEACAKHLHDPPPHVRELAADAPASLDGLVDRLLAKSPAARAESMREVERAFSA
                     IAGNQPVAFAETLASMQTSAAPLPITPPQGNRPLTHPGRVSTSVMPTSPDTRRPRTAL
                     PLLAKLVLGVV"
     gene            1062..>2984
                     /locus_tag="VMZ53_29330"
     CDS             1062..>2984
                     /locus_tag="VMZ53_29330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HUS32653.1"
                     /translation="MGDIRMGARSVVTLFLLAGCRPAAQPAPAPATKPMPTPVATGSA
                     TPTPVAAGSATPVVDAPAPPPGPPVTTMTLAEVGLEATSLDRSVDPCVDFYQFACGGW
                     LAKNDIPADRARWGRLSEIDEKNLASLKTLLDNDAKWNATDPIGNKLGDFYASCMDDA
                     ALEKIGTAPFKPLLDKAAKVKDAKSWLAMVGELHKIAVPVVWTVTADVDNKDSSTYVT
                     MLDTGGLGLPDRDYYLDDKFKDKVDAYREHVQRMLALAGVQKPDAAAADVIAIETALA
                     KLTKTRVERRDPQAAYNPMDVKALGKSAKSVDWNAYFKALGIKPSKKIIVGTPKFFAG
                     LDALRKAQSPAKWQSYFTYHLLAANAIALPKAFDDEAFALEKVLTGVAAQPPRFKRCI
                     AATTEALGELLGKQYVDKYFPGQARATAVTLVDAVVKELDADLAQLDWMSDATKQVAR
                     DKLTKIVRMIGYPDKWRAYDYPIKRDDLGGNYMRAAAAETHRVLVRSGKPVDRAEWLM
                     NTFTVNAYYNPPTNNTALLAGILQPPFFGQERGIPANLGGIGMVIGHELTHGFDDEGS
                     QYDAKGNLVNWWKPDDQSKFVAKGQCVAKMYDSFEVLPKQFVNGKLTLGENIADLGGV
                     KMAFRAYRSLRKDATRQ"
BASE COUNT          544 a          966 c         1000 g          474 t
ORIGIN      
        1 gacaaccccg agcacgagct tcgcgaggag cggcagcgcg gtgcgaggcc gccgtgtgtc
       61 cgggctcgtc ggcatgacac tcgtgctgac gcgtccggga tgcgtcagcg ggcgattgcc
      121 ctgcggcggt gtgatcggaa ggggcgcggc cgacgtctgc atcgaagcga gcgtctcggc
      181 gaatgcgacg ggctggttgc cggcgatcgc ggagaacgcg cgctcgacct cgcgcatgga
      241 ctcggcgcgc gcagcgggcg acttcgcgag taagcgatcg acgagaccat cgagggatgc
      301 cggcgcgtcg gcggcgagct cgcggacatg aggtggcggg tcgtgcagat gcttcgcgca
      361 cgcctcggcg atgttcgaca cgatgaacgg cgggcggccc gtcgccatct cgaaggtcac
      421 gcagccgagc gaatagaggt ccgcacgcca gtcgaccttc gaggcgtcgc cccattgctc
      481 gggcgccatg tacgcgggcg tgcccatcgt gccggtcgtg cgcggcgaga ccacggcgag
      541 cgtgcccgtc agcttcgcga tgccgaagtc gaggatcttc acgcgatagc cgcgcgccag
      601 ctctcgatcg ggaacgacga agatgttgtc gggcttgagg tcgcggtgcg tgatgccggc
      661 cgagtgcgtc gcgtcgagca cgctcgcgac ctggcgcccg atctcgcaga ggtccgcgag
      721 cggtagccgt ttggcttgtg ctatgcgctg cgcgagcgat tcgccgtcga ggaattccat
      781 cacgagatac gcatggccac cgtggtggcc gacgtcgaag atcttgacga tgccggcgtg
      841 ctggatgcgg ctgaccgcgc gcgcctcgtt gaagaaccgc tggacgtgct cggtatcctc
      901 ggagaccgcg gcgttgagca gcttgatcgc gaccttcgtg ccgatgctgg tttgctcggc
      961 cagccagacc tcgcccatgc cgccacgacc aagacgtgag acgatcttga agtttccgat
     1021 cgtctccccg atcacgctgt ctcgatggta tcaccggcga cgtgggggac atccgcatgg
     1081 gtgctcgttc cgtagtcaca ctgttcttgc tcgcggggtg tcgtccagcc gcgcagcctg
     1141 cgcctgcgcc cgcgaccaag ccgatgccta cgccggtggc gacgggaagc gcgacgccaa
     1201 cgccggtcgc cgccggtagc gccacgccgg ttgtcgatgc tcctgcgcca ccgcccgggc
     1261 cgcccgtcac gacgatgacg ctcgccgagg tcgggctcga agcgacgtcg ctcgatcgca
     1321 gcgtcgaccc gtgcgtcgac ttctatcagt tcgcgtgcgg cggctggctc gcgaagaacg
     1381 acatccccgc ggatcgcgcg cgatggggcc ggctctccga gatcgacgag aaaaacctcg
     1441 cgtcgctgaa gacgctgctc gacaacgacg cgaagtggaa cgcgaccgat ccgatcggca
     1501 acaagctcgg cgatttctac gcgtcgtgca tggacgatgc tgcgctcgag aaaatcggaa
     1561 ccgcgccgtt caagccgctg ctcgacaagg ccgcgaaggt gaaggacgcg aagtcgtggc
     1621 tcgcgatggt cggcgagctg cacaagatcg cggtgcccgt cgtgtggacg gtgactgcgg
     1681 acgtcgacaa caaagactcg tcgacgtacg tcacgatgct cgacacaggc gggctcggct
     1741 tgccggatcg cgactactac ctcgacgaca aattcaaaga caaggtcgac gcgtatcgcg
     1801 agcacgtgca gcgcatgctg gcgctcgcgg gtgtgcaaaa gcccgacgcc gcggccgccg
     1861 acgtgatcgc gatcgagacg gcgctggcca agctcacgaa gacgcgcgtc gagcggcgcg
     1921 atccacaggc ggcgtacaac ccgatggacg tcaaggcgct cggcaagagc gcgaagtccg
     1981 tcgattggaa cgcgtacttc aaagcgctcg gcatcaagcc gagcaagaag atcatcgtcg
     2041 gtacgccgaa gttctttgct ggtctcgacg cgcttcgcaa agcgcaatcg cccgccaagt
     2101 ggcagagcta cttcacgtat cacctgctcg ctgccaatgc gatcgcgctg ccgaaggcgt
     2161 tcgacgacga agcgttcgcg ctcgagaaag tcctgaccgg cgtcgccgcc cagccgccgc
     2221 gcttcaagcg ctgcatcgct gcgaccaccg aagcgctcgg cgagctgctc ggcaagcaat
     2281 acgtcgacaa atactttcct ggccaggcgc gcgcgaccgc ggtcacgctc gtcgatgcgg
     2341 tcgtcaaaga gctcgatgct gatctcgcgc agctcgattg gatgagcgat gcgacgaagc
     2401 aagtcgcgcg cgacaagctc acgaagatcg tccgcatgat cggctatccc gacaagtggc
     2461 gcgcgtacga ctacccgatc aaacgcgacg acctcggggg caactacatg cgcgcggctg
     2521 ccgccgagac ccatcgcgtg ctcgtgcgct cgggcaagcc cgtcgaccgt gccgagtggc
     2581 tgatgaacac gttcacggtc aacgcctatt acaacccgcc gacgaacaac accgcgctcc
     2641 tcgcgggcat cctgcagcca ccgttcttcg gtcaggagcg cggcatcccc gcgaacctcg
     2701 gcggcatcgg catggtcatc ggccacgagc tcacgcacgg gttcgacgac gagggctcgc
     2761 agtacgatgc gaagggaaac ctcgtgaatt ggtggaagcc cgacgatcaa tcgaagttcg
     2821 tcgcgaaagg gcagtgcgtc gccaagatgt acgactcgtt cgaggtgctg ccgaagcagt
     2881 tcgtcaacgg caagctcacg ctcggcgaga acatcgcgga tctcggcggt gtgaagatgg
     2941 cgttccgcgc gtatcgctcg ctgcgcaaag atgcaactcg ccag
//
LOCUS       DATLBZ010000596         4311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7181691, whole genome shotgun sequence.
ACCESSION   DATLBZ010000596 DATLBZ010000000
VERSION     DATLBZ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7181691"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VMZ53_29335"
     CDS             complement(<1..772)
                     /locus_tag="VMZ53_29335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HUS32654.1"
                     /translation="MLRSATLEQTWRRFAPRAKRAGITRIADLTGLDTLGIPVFAAIR
                     PMGLSLSTQQGKGITPDAARISALMESLETWSAEHIALPIVRGSYRALSKKYARSPAT
                     RRRPSATHDDRPRVVDVRTLPRTAKRLDLDERWAWIEGRDIVSGDRVLVPLQAVSLDT
                     TFDKPPVFDVSSNGLGAGNSLGEATLQGVCEVLERDAEAAWRRSGGDRRLILDTITDA
                     TCLDLIARITRTGARIFIWDLEPRGNICAIGCGIMEDPN"
     gene            800..1315
                     /locus_tag="VMZ53_29340"
     CDS             800..1315
                     /locus_tag="VMZ53_29340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32655.1"
                     /translation="MRVHAAVLGIACAALALSAVAGCSTGGSYVKSDREEGAADRGET
                     NGRMFDFVSNMPEGDDWQIRIRDNSLWAAYGDREKVKELGTKPLTKKEANKVWELVEA
                     VDIPSRKKGYEDEDEGWVSLRLREPGDEQHDIFTIYVSRADAEDDQEVLDLAAYLQDL
                     VEKHFKKKPEF"
     gene            1315..1794
                     /locus_tag="VMZ53_29345"
     CDS             1315..1794
                     /locus_tag="VMZ53_29345"
                     /inference="COORDINATES: protein motif:HMM:NF020544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HUS32656.1"
                     /translation="MSTTHGILIALSSKRLALLQNEPETLEDVIEARHEDEIPGLLDM
                     STHWETLDMLVSGRGRDPVLGDVFLARSGKPLSVDTAFEQALVLGPQRVAEVAAKLAE
                     VPVTLVRERYAQLEEAKNPGKPVNVKKQAEIELLYEEIVDLYVEAAKQKQSMLAVLV"
     gene            complement(1796..3091)
                     /locus_tag="VMZ53_29350"
     CDS             complement(1796..3091)
                     /locus_tag="VMZ53_29350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32657.1"
                     /translation="MPRLSSSEVFTPTSYPTHTYVVRDDLHHERLLREWIRQSTQIAS
                     VSGPSKAGKTVLVQRVVGEGNLFTVSGASVRTPDQLWDRVLDWAGEPHAMTASQVDAS
                     TESESHERGANLGVAGFAQASGRGTHGHQQSETATTQATVNRRGLPQVVQQLANSPYT
                     ILLDDFHYIPQAIQNDVAQQLKDAASRGVRIGVASVPHRSDNLVRALPELRGRVLAVD
                     MDYWNKRDLLEIPKLGCPLLGLEVDQASLQMFAIEAAGSPQLMQSICQWMCNHLGVRE
                     TAEPLRAIQLDETARKEILFLTSCTVDFRSLVRALISGPKVRPGERRSYVHNDGRVGD
                     VYLSIMRAVALDPPKLSFDRSELANRLDALTKGASPDAASVLRACGTLAQIAQGFAAP
                     TGPSLEWDEQAQVLDVPDPYLLFYLRWSVALEREADALM"
     gene            3154..4305
                     /locus_tag="VMZ53_29355"
     CDS             3154..4305
                     /locus_tag="VMZ53_29355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS32658.1"
                     /translation="MRIAETLRMDRKGLDLLEASDPRTERIWRALEAVAQPSYFLSWA
                     WIENWLTSLPAEEQPSLAVVCDGGEPAAAFFLAKRRVRRNFLMSKNALFFNATGSPEH
                     DELSIEHNGLLAAPGARKSLSGILDLIPDDWDELYLPALDRYAFDDLGAPSSPLSARY
                     KVQIEREANAPFVDLEAVRNVEGGYEALLPASTRTHLRSTRGVLGDVEVEVAADEAQA
                     LDIYSELLRLHEKRWLSRGLRGAFSEPWFENFHRNLILKRFAHGEIQLVRVSSGNSTL
                     GCLYNLVSRNRVTFYQCGLASFDDPAIKTGYLCHAAAIEYNALAGHSTYDLVGGRARH
                     KEALATGSTRLVWLRVQRPSSRLVTFEDNVRKVKDFFAASNAGLILRPA"
BASE COUNT          737 a         1367 c         1463 g          744 t
ORIGIN      
        1 cgttcgggtc ttccatgatc ccgcagccga tcgcgcagat gtttccgcgc ggctcgagat
       61 cccagatgaa gatgcgcgca cccgtgcgcg tgattcgcgc gatgagatca aggcaggtgg
      121 cgtcggtgat cgtgtcgagg atcaggcgcc ggtcgccgcc gctgcgtcgc catgcggctt
      181 cggcatcgcg ctcgaggact tcgcacacgc cttggagcgt cgcttcgccg agcgagttgc
      241 ctgcgccgag gccgttggac gagacgtcga agacgggcgg cttgtcgaac gtcgtatcga
      301 gcgagacggc ttgcagcggc acgagcacgc gatcgcccga cacgatgtcg cggccctcga
      361 tccacgccca ccgctcgtcg agatctaaac gtttcgcggt ccgtggcagc gtacgtacat
      421 cgacaacgcg ggggcggtca tcgtgcgttg cggatgggcg gcggcgggtc gcgggtgagc
      481 gcgcgtactt cttcgagagc gcgcggtagc tgccgcggac gatcggtaac gcgatgtgct
      541 cggcggacca cgtctcgagc gattccatca gcgcggagat gcgcgcggcg tcgggcgtga
      601 tgcctttgcc ttgttgcgtc gacagggaga ggcccatcgg gcggatggcc gcgaagaccg
      661 ggattccgag cgtatcgagg cccgtgaggt cggcgatgcg cgtgatcccg gcgcgtttgg
      721 cccggggcgc aaatcgccgc catgtctgct cgagagttgc gctgcggagc atcgcgcccg
      781 ccggtgctat aacccagccg tgcgggtcca cgctgctgtc cttggtatcg cttgtgctgc
      841 gcttgcgctg agcgcggttg cgggctgctc gaccggcggc agctacgtga agagcgaccg
      901 cgaggaaggt gccgcggatc gcggtgagac gaacggccgg atgttcgact tcgtgtcgaa
      961 catgcccgag ggcgatgact ggcagattcg cattcgcgac aactcgctgt gggccgcgta
     1021 tggcgatcgc gagaaggtga aggagctcgg gaccaagccg ctgacgaaga aagaggcgaa
     1081 caaggtatgg gagctcgtcg aggccgtcga cattccgtcg cgcaagaagg gctatgaaga
     1141 cgaggacgag ggctgggtga gcctgcgtct gcgcgagccc ggtgacgagc aacacgacat
     1201 tttcacgatc tacgtctcgc gggccgatgc ggaagacgac caagaggtat tggacctcgc
     1261 cgcgtacctg caggatctgg tagagaagca cttcaagaag aagccggaat tctgatgtcg
     1321 accacccatg gaatcttgat cgcgctgtcg tcgaagcggc tcgcgttgtt gcagaacgag
     1381 cccgagacct tggaagacgt catcgaggcg aggcacgagg acgaaatccc gggcctgctc
     1441 gacatgagca cccactggga aacgctcgac atgctggttt cggggcgcgg ccgcgatccg
     1501 gtgctcggcg acgtgttcct cgcgcgcagc ggcaagccgt tgtccgtcga cacggcgttc
     1561 gagcaagcgc tcgtcctcgg gccgcagcgc gtcgctgaag ttgcggcgaa gctcgccgag
     1621 gttccggtga cgctcgtccg cgagcgctac gcgcagctcg aagaagcgaa gaatcccggc
     1681 aagccggtca acgtgaagaa gcaagccgag atcgagctgc tgtacgaaga gatcgtcgac
     1741 ctctacgtcg aggctgcgaa gcagaagcag tcgatgctcg ccgttctcgt ctgaattaca
     1801 tcagcgcatc ggcttcgcgc tcgagcgcga ccgaccaccg caggtagaac agcaagtacg
     1861 ggtccggcac gtcgaggact tgggcttgtt cgtcccattc gagcgacggc ccggttggcg
     1921 ctgcgaagcc ttgagcgatc tgcgcgagcg tgccgcacgc acgaaggacg ctggctgcgt
     1981 cgggcgatgc gcccttggtc agcgcgtcga gacgattcgc gagctcgctg cgatcgaacg
     2041 acagcttcgg cggatcgagc gcgaccgcgc gcatgatcga gagatagaca tcgccgacgc
     2101 gaccgtcgtt gtgaacgtac gagcgacgct cgccgggtcg caccttcggg ccggagatca
     2161 gcgcgcgcac gagcgagcgg aagtcgacgg tgcacgatgt gagaaacagg atctctttgc
     2221 gcgccgtctc gtcgagctgg atcgcacgca gcggctccgc ggtctcgcgt acgccgaggt
     2281 gattgcacat ccactggcag atcgactgca tcagctgcgg cgacccggct gcttcgatcg
     2341 cgaacatctg cagcgatgct tgatcgactt cgagcccgag cagcgggcag ccgagcttgg
     2401 gaatctcgag caggtcgcgc ttgttccagt agtccatgtc cacggcgagc acgcggccac
     2461 gaagctcggg cagcgcacgc acgaggttgt cgctgcgatg cggaacgctc gcgacgccga
     2521 tccgcacgcc gcggctcgcg gcatctttga gctgctgcgc gacgtcgttc tggatcgcct
     2581 gcgggatgta gtggaagtcg tcgagcagga tcgtgtacgg cgagttcgcg agctgctgca
     2641 cgacttgcgg caagccacgg cggttgaccg tcgcttgcgt cgtcgcggtt tcgctctgct
     2701 gatggccgtg cgtgccgcgc cccgacgcct gcgcgaagcc tgcgacgccg aggttcgcgc
     2761 cgcgctcgtg cgattcactc tccgtcgacg cgtcgacctg gctcgccgtc atcgcgtgcg
     2821 gctcgcccgc ccagtcgagc acgcgatccc agagttggtc gggcgtgcgc acggatgcgc
     2881 cgctcaccgt gaacaaattg ccttcgccga cgacacgctg cacgagcacc gtcttgcccg
     2941 ctttcgacgg cccgctcacc gaggcgattt gcgtcgactg ccggatccac tcgcgcagca
     3001 agcgctcgtg atggaggtca tcgcgaacca cgtatgtatg cgtcgggtac gaggtcggcg
     3061 tgaacacctc ggaggacgac aagcgcggca tggcgctcat catgtggcat gtgcgcacgg
     3121 tgcgcagcca atccgcgcaa gagacttccg ggcatgcgga ttgctgaaac actccgcatg
     3181 gaccgcaagg gcctcgacct cctggaagcg agcgatccgc gcacggagcg gatctggcgt
     3241 gcgctagagg cggttgcaca gccttcgtac ttcctgtcgt gggcgtggat cgagaactgg
     3301 ctcacgtcgc tgccggcaga agagcagcct tcactcgctg tcgtgtgcga cggtggtgag
     3361 cccgctgcgg cattcttcct cgcgaagcga cgcgtccggc gtaacttcct gatgtcgaag
     3421 aacgcgctgt tcttcaatgc gaccgggtcg ccggagcatg acgagctctc gatcgagcac
     3481 aacgggttgc tcgcggcgcc cggtgcgcgc aagtcgctct cgggaattct cgacctcatc
     3541 cccgacgatt gggacgagct gtacttgccg gcgctcgatc gctacgcgtt cgacgatctc
     3601 ggcgcgccct cctcgcctct ctcggcgcgc tacaaagtgc agatcgagcg cgaggcgaat
     3661 gcgccgttcg tcgacctcga agcggtgcgc aacgtcgaag gcggctacga agcgctgctt
     3721 cctgcgagca cccgcacgca cctgcgcagc acgcgcggag tgctcggcga cgtcgaagtc
     3781 gaggtcgcgg ccgacgaagc gcaggcactc gatatctaca gcgagctgct tcgtctgcac
     3841 gagaagcggt ggttgtcgcg cggtctgcgc ggcgcgttct ccgagccgtg gttcgagaac
     3901 ttccaccgca acttgatcct caagcggttc gcccacggcg agatccagct cgtgcgcgtc
     3961 agcagcggca actcgacgct cggctgtctc tacaacctcg tctcgcggaa tcgcgtcacg
     4021 ttctaccagt gcgggctcgc gtcgttcgac gaccctgcga tcaagaccgg ctacctctgc
     4081 cacgcagcgg cgatcgagta caacgccctc gcaggtcact cgacgtacga cctcgtcggc
     4141 ggccgcgctc gtcacaaaga agcgctcgcg accggctcga cccggcttgt ctggctccgc
     4201 gtccagcgtc cgtcgagccg cctcgtgacg ttcgaggaca acgtccgcaa ggtgaaggac
     4261 ttcttcgccg cgagcaacgc aggcctcatt ctgcgtccgg cgtaacggac t
//
LOCUS       DATLBZ010000597         4157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3476524, whole genome shotgun sequence.
ACCESSION   DATLBZ010000597 DATLBZ010000000
VERSION     DATLBZ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3476524"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VMZ53_29360"
     CDS             complement(<1..611)
                     /locus_tag="VMZ53_29360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32659.1"
                     /translation="MDDRQLAKTVADGGRLRATLLELWQRTRMDWGFVTDRLAVTFRN
                     EKWIGGHDRRFISETLYGLVRHLRRIDLALERGRRVKKPPRDLERLLALLVLEKLLEP
                     AAAAKVESDLDWNAVARVDEVIANERNLEKRIALAASLPDWLAKRFVANWGAEAEPLA
                     LALNQRAPMTIRANTLMTSRDALAKELASEKLDTHPGAWSDSAL"
     gene            654..2849
                     /locus_tag="VMZ53_29365"
     CDS             654..2849
                     /locus_tag="VMZ53_29365"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32660.1"
                     /translation="MRFVVVALVISACGRIGFDEQQPPGATDLHRGRMAAGLGFTCVA
                     QSDGDAFCWGRGDFHQLGGATTLAPTPIHVNLPAMTRISAGGVGACGIDDSGALWCWG
                     SNLAGELGQGDLVDRVDPIVVPLASKAIDVAVGYTHVCAVLEDTSVWCWGENVDGNVG
                     VDVAARFVMEPTRVFDRAVQIGAGFYHTCALRDDGSIWCWGNNAYGQLGLGATEPNAS
                     STPRKVPNVDAVGLAVGAAHTCVLVAGGRYRCWGSNAEGELGSPDAVDPSGREPTPFP
                     PSELSGLVALTAGTLVTCGLHRDGRMWCWGDNRAGELGNGNRFVSRTPLQVPISGAIA
                     IAAGVHHTCALRADESVTCWGLGSNGQLGDGHLAELAPRSVALPGAATSLAVGSQHAC
                     AIVGGEAYCWGEDANLQLGFNQVSDPQIARAEPTLVPGLASLQRLSLGADHSCATVGD
                     GSAVCWGANSSGQLGHGDYLYGKLPAAMMGLTLPVLEIAAGGVFTCVLDSAGVACVGD
                     NYYDELGTTGVQSSPTPVRLALGPVAAIAAGFTHACALENPTGLATCWGHNDYGQAGA
                     NTVTVSTPPVAVAGGHTFITIDGGGNHTCAVEMPNDQTWCWGDGRAGQLGTGAVLEVA
                     PAPAMVAGGMTTTQVSAGIDSTCAIEATGNVVCWGANYAGQLGTNDIRAISTPVANGL
                     VASEVHAGSGFACARTLVGDVYCWGDNQSGQIGIGKFSRSLVPVDATFP"
     gene            2925..>4157
                     /locus_tag="VMZ53_29370"
     CDS             2925..>4157
                     /locus_tag="VMZ53_29370"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HUS32661.1"
                     /translation="MSTAPPPPTAPSSAKTEVSLKDRGNLFDVATKQIDKAMKLINID
                     HDVAEILGQPKNELIVNFPVRRDNGKFEMFKGYRVQHNNILGPYKGGMRYHHEANLDE
                     MKALASWMTYKSALHEIPFGGGKGGVKFDPRQCSKNELERITRRFTAALGSNIGPDFD
                     IPAPDVNTNSQTMVWMMDTYMNIVGAGDRNTVRGVVTGKSVTAGGSYGRAEATGQGVV
                     HCITEWAKDKNFNLDGCHVIIQGFGNVGSYAARLLSQKGAVVIGVGDVGGYIANTEGI
                     NPHKLGEHVHKTGSVSGYKGASKITREEFFSLDAHIFVPAALELEIGIAEAKALKVKM
                     VVEGANGPTYPEAEEIMIDKGIDVIPDILCNSGGVIVSYYEWLQNKRAEQWEHADVLG
                     RLERRMKQTYANVRQYATA"
BASE COUNT          719 a         1345 c         1406 g          687 t
ORIGIN      
        1 agcgcgctgt cgctccacgc tcccggatgc gtgtcgagct tctcgctcgc gagctccttg
       61 gcaagcgcgt cgcgcgacgt catgagcgtg ttcgcgcgaa tcgtcatcgg cgcgcgttga
      121 ttgagcgcga gcgcgagcgg ctcggcctcc gcgccccaat tcgcgacaaa tcgcttcgcg
      181 agccaatcgg gcagcgacgc ggcgagcgcg atgcgcttct cgaggttgcg ctcgttcgcg
      241 atcacctcgt cgacgcgggc gactgcgttc caatcgagat cgctctcgac cttcgccgcg
      301 gccgcgggct cgagcagctt ctcgagcacg agcagtgcga gcagcctctc gagatcgcgc
      361 ggcggcttct tgacgcgccg cccacgctcg agcgcgagat cgatccggcg cagatggcgc
      421 acgaggccgt agagcgtctc gctgatgaag cgccggtcgt gaccgccgat ccacttctcg
      481 ttgcgaaacg tcaccgcgag tcgatcggtg acaaagcccc agtccatgcg cgtgcgttgc
      541 cacagctcca gcagcgtcgc gcgcaagcgc ccgccatcgg cgacggtctt tgcgagctgt
      601 cgatcgtcca cgccgcgtgt atacacgggg cccggctgtg cgacgatccg ttcgtgcgat
      661 tcgtcgtcgt tgcgctcgtg atctcggcgt gcggcaggat cggcttcgac gaacagcaac
      721 caccgggtgc gaccgattta catcgcgggc gcatggcggc gggcctcgga tttacgtgcg
      781 tggcgcagag cgatggcgat gcattctgct ggggacgcgg cgactttcat cagctcggtg
      841 gcgcgacgac gctcgcaccg acgccgatcc acgtgaacct tcccgcgatg acgcgcatct
      901 ccgcgggtgg tgtgggcgcg tgcggcatcg acgatagcgg cgcgctgtgg tgctggggaa
      961 gcaacttggc gggcgagctc ggccagggtg atctcgtcga tcgcgtcgac ccgatcgtcg
     1021 tcccgctcgc gagcaaggcg atcgatgtcg ccgtcggcta cacgcacgtg tgcgccgtgc
     1081 tcgaagacac gagtgtgtgg tgctggggcg aaaacgtcga cggcaacgtg ggtgtcgatg
     1141 tcgcagctcg gttcgtcatg gagccgacac gcgtcttcga tcgcgcagtg caaatcggcg
     1201 ccggcttcta tcacacgtgc gcgctgcgcg acgacggctc gatctggtgc tggggcaaca
     1261 acgcgtacgg ccagctcggc ctcggtgcga ccgagcccaa tgcgagctcg actccgcgca
     1321 aggtgccgaa cgtcgacgcg gtcgggctcg ccgtcggagc ggcgcatacc tgtgtgctcg
     1381 tcgccggtgg ccggtatcgc tgctggggct ccaacgcgga gggcgagctc ggatcaccag
     1441 atgcagtcga tccttcgggc cgcgagccaa cgccgtttcc tccctcggag ctgagcgggc
     1501 tcgtcgcgct cacggccggt acgctcgtga cgtgcggcct gcatcgcgat ggccggatgt
     1561 ggtgctgggg agacaatcgc gccggcgagc ttggtaatgg aaatcgattc gtgtcgcgga
     1621 ccccgttgca agtcccgatc agcggcgcga tcgcgatcgc ggctggtgtg catcacacgt
     1681 gcgcgttgcg cgccgacgag agcgtaacgt gttgggggct cggctcgaat ggtcagctcg
     1741 gcgacggtca tctcgcggag ctcgcgccgc gcagcgttgc actgccgggt gccgcgacgt
     1801 ctctcgcggt cggatcgcag catgcgtgcg cgatcgtcgg cggcgaggcg tattgctggg
     1861 gcgaggacgc gaacttgcag ctcggattca atcaggtcag cgatccgcag atcgcccgcg
     1921 ccgaaccgac actcgtacct gggcttgctt cgttgcagcg gctgtcgctc ggcgccgatc
     1981 acagctgcgc gacggtcggg gacggctccg cagtctgctg gggcgcgaac tcgagcgggc
     2041 agctcggcca cggcgactac ctgtacggga agctgcctgc agccatgatg gggctgacgt
     2101 tgccggtctt ggagattgcc gccggcggcg tgttcacgtg cgtgctcgat tcggcgggcg
     2161 tcgcgtgcgt tggcgacaac tactacgacg agctcgggac caccggcgtg cagtcgtcgc
     2221 cgacaccggt gcgacttgcg ctcggtccgg tcgcagcgat cgctgcgggc ttcacgcatg
     2281 catgcgcgct cgaaaacccg accggcctcg cgacgtgctg gggccataat gattacggtc
     2341 aggccggcgc gaacaccgtg acggtgtcca cgccaccggt cgcggttgcc ggcggtcata
     2401 cattcatcac gatcgacggc ggcggcaatc acacgtgcgc ggtcgagatg ccgaacgacc
     2461 agacgtggtg ctggggtgat ggtcgagccg gtcagctcgg caccggtgct gtcctggaag
     2521 tcgcgcccgc acccgcaatg gttgcaggtg gaatgacgac gacccaggtc agcgcgggca
     2581 tcgacagcac gtgcgcgatt gaagcgaccg gtaacgtcgt gtgctggggc gcgaactacg
     2641 ccggtcagct cggcacgaat gacatccgag cgatcagcac gccggtcgcc aacggcctcg
     2701 tggcgagtga ggtccacgcc gggtccgggt ttgcgtgcgc gcgaaccctc gtcggggatg
     2761 tgtactgctg gggcgacaac cagtccggtc agatcggcat cggcaagttc tcgcgttcac
     2821 tggtccctgt cgacgcgacg tttccgtgac gccgcgcgta atgcctgaaa tggttagcgg
     2881 aattgattgc ccctaccggg gccggagcgg tacgccttcg gcacatgagt actgcaccgc
     2941 cgccgccgac cgcaccgtca tcggcgaaga ctgaagtgag cctcaaggac cgcggcaatc
     3001 tcttcgatgt cgcgaccaag caaatcgaca aggcgatgaa gctgatcaat atcgatcacg
     3061 acgtcgccga gatcctcggc cagccgaaaa acgagctgat cgtcaacttc cccgtccgcc
     3121 gcgacaacgg gaagttcgag atgttcaagg gctaccgagt ccagcacaac aacatcctcg
     3181 gcccgtacaa gggcggcatg cgctaccacc acgaggcgaa cctcgatgag atgaaggcgc
     3241 tcgcgtcgtg gatgacgtac aagagcgcgc tccacgagat tccgttcggc ggtggcaagg
     3301 gcggcgtgaa gttcgacccg cgccagtgca gcaagaacga gctcgagcgc atcacgcgcc
     3361 gcttcaccgc cgcactcggc agcaacatcg gccccgactt cgacatccca gcacccgacg
     3421 tgaacaccaa cagccagacg atggtctgga tgatggacac gtacatgaac atcgtcggcg
     3481 ccggtgaccg caacacggtc cgcggtgtcg tcacgggcaa gagcgttacc gccggcggct
     3541 cgtacggccg cgcggaagcg acgggccaag gcgtcgttca ctgcatcacc gagtgggcga
     3601 aggacaagaa cttcaacctc gacggttgcc acgtcatcat ccaaggcttc ggcaacgtcg
     3661 gcagctacgc cgcccgcctc ctctcgcaga agggcgcagt cgtcatcggc gtcggcgacg
     3721 tcggtggcta catcgcaaac accgagggca tcaacccgca caagctcggc gagcacgttc
     3781 acaagaccgg ctccgtctcc ggctacaaag gcgcgtcgaa gatcacgcgc gaagagttct
     3841 tcagcctcga cgcgcacatc ttcgtgccgg ccgctctcga gctcgagatc ggtatcgccg
     3901 aagcaaaggc gctcaaggtg aagatggtcg tcgagggcgc gaacggcccg acgtatcccg
     3961 aagccgaaga gatcatgatc gacaagggca tcgacgtgat ccccgacatt ctctgcaact
     4021 cgggcggcgt gatcgtcagc tactacgagt ggctgcagaa caagcgcgcg gagcagtggg
     4081 agcacgccga cgtgctcggg cgcctcgagc gccgcatgaa gcagacgtac gcgaacgttc
     4141 gccagtacgc gaccgcg
//
LOCUS       DATLBZ010000598         6847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7873049, whole genome shotgun sequence.
ACCESSION   DATLBZ010000598 DATLBZ010000000
VERSION     DATLBZ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7873049"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VMZ53_29375"
     CDS             complement(<1..1427)
                     /locus_tag="VMZ53_29375"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUS32662.1"
                     /translation="MPNRLATESSPYLRQHADNPVEWWPWGEEAFAEAKRLDKPVFVS
                     IGYAACHWCHVMAHESFEDAATAQLMNELFVSIKIDREERPDVDAIYMNAIQVMGEGG
                     GWPLSAFCLPDGRPYYLGTYFPKAPRFGRPSFSDLLRAMSDAFKTRRADAEDNALALI
                     DGLQRVDAHYRRGAEMGQVGKLDASLLIAAGKQLAERCDPKHGGLGGAPKFPSSSSHD
                     LLARASRLPFGDVAGKAFEQWARGMAEGGIYDHLGGGFARYSVDAKWLVPHFEKMLYD
                     QAQLLGIFATVVAKSGPFAARAEEVIGETVAFLERELSDKAGGLWSSLDADSEGEEGK
                     FYVWTPDEVRAALGPVNALVFCQAYGVTDAGNFEHGATVLSRVTPRSSPHEEEQLAEL
                     RARLLDARGKRVRPGTDDKVLAGWNGLALTGLCAAWRATGHAPALELAKRVGGFLRDH
                     MIRDGRVARVFHEGKTKELDGTLDD"
     gene            complement(1452..2357)
                     /locus_tag="VMZ53_29380"
     CDS             complement(1452..2357)
                     /locus_tag="VMZ53_29380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32663.1"
                     /translation="MKSAKTYVLLERLASGGMADIFLAREIASAGLVRHCVVKRIRRD
                     CESDARYVQLFHEEMRIVSRLQHPNIPHVLDIGRIDGSSFMAMEYVHGVTLGGLLGRA
                     RAASTMLPLSTILPIAAGIACALQHVHDRGVVHHDVSLSNVMVTFDGHVKLIDFGIAS
                     TRNDVCPGMIKGKPAYMAPERRVGALGDERSDLFSLGVVLWELLCQMRLAPGDSVVPP
                     STFRPMLPVQLDHLVAALLADDPEARHASASCVLDSIERIARHTSTLMTPTSTRHVLH
                     TLFGATPEPWTVDDRTEVYEQRLAS"
     gene            complement(2354..3904)
                     /locus_tag="VMZ53_29385"
     CDS             complement(2354..3904)
                     /locus_tag="VMZ53_29385"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HUS32664.1"
                     /translation="MLAALCVLTASAIASADVDRFALIVGDNDGLADEQRLRFAESDA
                     ARFAELLGEVGGIPDENQVVLRGKSADEVTRALIAMNERIRMRQQKGNSAMLFVYYSG
                     HGDAESLHLGDTRLRLTDLEKLLRGSSAQVRVLVIDACRSGSLTRVKGGKPAPALAIS
                     SADDLPGEGVIVLTASTLGEDAQESDELGGSFFTHYLVSAMRGAADDNGDHIVTVAEA
                     FRFTRDQTIVASSRTLSGIQHPTFHYDLRGRSDVALADLAKDSERGTVTLPADATWLV
                     FRTEPAKAVVGEVAAGATQRSLSLRAGTYLLRGRTRDAMLEGTVKIAAGDSVNVETSS
                     LSRLTYARLVRKGRGEVLPTVQGPFAGLAYWQLAPGATLGWTFVRPSITISPRVVGAY
                     HFRPYRMTPTVTSYPLKTYLGAVDVRVTRAWELRHFAVDFGVTIGASEQLEKGEYPLG
                     VQSLTIEWRSVHADLTAGISVPLGGRVYLASELAAQAHIAWEPRKSGDRPEGAFGILA
                     NFVAGVWL"
     gene            complement(3910..5421)
                     /locus_tag="VMZ53_29390"
     CDS             complement(3910..5421)
                     /locus_tag="VMZ53_29390"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HUS32665.1"
                     /translation="MAAVVCGLAAPARAEVERFAVVIGHDEGLADEQRLQFAEKDASQ
                     VADVLDEIGGVPAENQVVLRGKNADQVRRALIDINDRIRSSGHDSVLVVYYSGHGDAE
                     SLHLGDTKLALRELEQLVRGSAASVRVLVIDACRSGSLTRTKGSKRAIDVDESLPADG
                     VIVFTASTLGEDAQESEKLGGSFFTHYLVSGMRGAADTNSDGVVTASEAFEYTREQTI
                     LESSRSSAGAQHPTFEYDLRGRADLTLSRLAPDNARGTLVVPPGATWLVVRTSPSKTV
                     IAEIGADAKQRKLSLRAGSYAVQGRTRDALLEGSVKVAAAKETSVDSDSLTRTMYARL
                     VRKGRGDVLPSVAGFFAGATVMALPGSLALPGVVGGFMYVLPAVTLSPRISAVHGWPD
                     RFDGGVANLLVAELRITRAWELPHFSVDLGGSLGGSYLDARESDHAFHDTPAVISRHA
                     GPMIGAAAGVTRPLSGRLFASAELALQSHFLIGDCCDEPGVLLDANLFLGGWL"
     gene            complement(5433..6125)
                     /locus_tag="VMZ53_29395"
     CDS             complement(5433..6125)
                     /locus_tag="VMZ53_29395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32666.1"
                     /translation="MSDHPSDLAWDQLLAGERDDAATLRAHAESCSVCGERLHMLKAG
                     HDAFAARPMPAFAARPKRRAWLYAAPVVVAALALVVVGLAKRPEQDDVRIKGTHDAKL
                     QLFAGAPGHLAPVRSGDRVAPGTYLQASYSAETAGFGAVISKDGAGSTNAYVPSTGTL
                     LVPLAAGVEHVFPESTELDDVLGTEHVVIIWCATQREVAPFIEAVRTGAVLAPEGCVA
                     STIDLAKQAVTP"
     gene            complement(6122..6580)
                     /locus_tag="VMZ53_29400"
     CDS             complement(6122..6580)
                     /locus_tag="VMZ53_29400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS32667.1"
                     /translation="MYRAYGHSVLRRARQMLVSDDDAAEVLQEIFMGLIARPGQFDGR
                     SSPSTFLYAVTTNACLTRIRDRKNRGRLIDQHVRPWSTEMDRRSPETATVVRSVLAQL
                     SEDEARTAVYYYLDGMSHAEIADLLGCSRRHVGNLLERVVGRLAGEERAS"
BASE COUNT         1175 a         2338 c         2255 g         1079 t
ORIGIN      
        1 taatcgtcga gcgtgccgtc gagctccttc gtcttgcctt cgtggaacac gcgagccacg
       61 cggccatcgc gaatcatgtg gtcgcgcagg aagccgccga cccgcttcgc gagctcgagt
      121 gcaggtgcat gccccgttgc gcgccacgcc gcgcagagcc ccgtcagcgc gagaccattc
      181 cagcccgcga gcactttgtc gtcggtgccc gggcggacgc gcttgcctct cgcatcgagc
      241 aatcgcgcgc gcagctccgc gagctgttct tcttcatgcg gtgacgaccg aggtgtcacg
      301 cgcgagagca ccgtcgcccc gtgctcgaag ttgcccgcgt ccgtcacgcc gtacgcctgg
      361 cagaacacga gtgcgttgac aggaccgagc gcagcgcgca cttcatcggg agtccacacg
      421 tagaacttgc cctcctcgcc ttcgctgtcg gcgtcgagcg aggaccacag gccgcctgct
      481 ttatccgaca gctcgcgctc gagaaacgcg actgtctcgc cgatgacttc ttcagcgcgc
      541 gcggcaaacg gaccgctctt tgcgacgacg gttgcgaaga tgccgagcag ctgcgcttgg
      601 tcgtagagca tcttctcgaa gtgcggcacg agccacttcg cgtcgacgga gtagcgcgcg
      661 aacccgccgc cgagatgatc gtagatgccg ccttcggcca tgccacgcgc ccattgctcg
      721 aacgccttgc cggcgacgtc gccgaacggc aagcgcgatg cgcgcgcgag caagtcgtgg
      781 ctcgagctcg acggaaactt cggcgcaccg ccgagaccgc cgtgcttcgg gtcgcagcgc
      841 tccgccagct gcttgcccgc cgcgatgagc agggacgcat cgagcttgcc gacctgtccc
      901 atctccgcgc cgcggcgata gtgagcatcg acgcgctgca ggccatcgat cagcgcgagc
      961 gcgttgtcct cggcatcggc gcgccgcgtc ttgaacgcgt ccgacatcgc gcgcagaaga
     1021 tccgagaagc tcgggcggcc aaagcgcggc gccttcggaa aatacgtgcc gaggtagtac
     1081 ggccggccat ccggcaagca gaacgctgac agcggccagc cgccaccctc gcccatcact
     1141 tggatcgcgt tcatgtagat cgcgtcgacg tcgggtctct cctcgcggtc gatcttgatg
     1201 ctcacgaaca gctcgttcat gagctgcgcg gtcgcggcat cctcgaaact ctcgtgcgcc
     1261 atcacgtgac accagtggca cgccgcgtag ccgatcgaga cgaacaccgg cttatcgagg
     1321 cgcttggcct cggcaaacgc ctcttcgccc cacggccacc attcgacggg attgtcggcg
     1381 tgctgccgca aatacggcga gctttcggtc gcgaggcggt tcggcatggc cgcaccatac
     1441 ggcacgaccg ctcatgacgc gagccgctgc tcgtacacct cggtgcggtc gtcgaccgtc
     1501 catggttcgg gcgtggcgcc gaacagcgtg tgcagcacgt gccgcgtcga ggtcggggtc
     1561 atgagcgtgc tcgtgtgacg ggcaatgcgc tcgatcgaat cgagaacgca agacgccgac
     1621 gcgtgccgcg cttccggatc atcagcgagc agcgcggcga cgagatggtc gagctgcacg
     1681 ggcagcatcg gacgaaacgt cgacggtgga acgactgagt cacccggagc aagccgcatc
     1741 tggcagagca gctcccacaa cacgacgccg agcgagaaca gatcgctgcg ctcgtcgccg
     1801 agcgcaccga cgcggcgttc cggtgccatg tacgccggct tgcctttgat catgcccggg
     1861 caaacgtcgt tgcgagtcga cgcgatgccg aagtcgatga gcttcacgtg cccgtcaaac
     1921 gtgaccatca cattcgagag cgacacgtcg tgatgtacga cgccgcggtc gtgaacatgc
     1981 tgaagcgcgc acgcgatgcc cgcggcaatc ggcaggatcg tcgagagcgg aagcatcgtg
     2041 gaggccgcgc gcgcacgccc gagcaatcca ccgagggtca cgccgtgtac gtattccatc
     2101 gccatgaagc tcgagccgtc gatgcggccg atgtcgagca catgcgggat gttcgggtgc
     2161 tgcaaccggc tgacgatgcg catctcctcg tgaaagagct gcacgtagcg cgcgtcgctc
     2221 tcgcagtccc ggcggatgcg cttgacgacg caatgccgaa cgagaccagc gctcgcgatc
     2281 tcgcgcgcga ggaagatgtc ggccatgcca ccgctcgcga ggcgctcgag cagcacgtac
     2341 gtcttcgccg acttcacagc cacactcctg cgacgaagtt ggcgaggatg ccgaacgcgc
     2401 cctccggccg atcgcccgac ttgcgtggct cccatgcgat atgtgcttgc gccgccagct
     2461 cactcgccag gtagacgcga ccgccgagcg gaaccgagat gcccgccgtg agatcggcgt
     2521 gtacagagcg ccattcgatc gtcagtgact ggacgccgag cggatactca cccttctcga
     2581 gctgctcact cgcaccgatg gtcacgccga aatcgaccgc gaagtgacgc agctcccacg
     2641 cgcgtgtcac acgaacgtcg accgcgccga gatacgtctt caacggataa ctcgtcacgg
     2701 tgggggtcat gcggtacggc cggaagtgat atgcgccgac gacgcgcggc gagatggtga
     2761 tcgacggccg gacgaacgtc caccccagcg tcgcgcccgg agcgagctgc cagtacgcga
     2821 gccccgcgaa tgggccctgc acggtcggaa gcacctcgcc gcgacccttg cggacgagtc
     2881 gcgcatacgt gaggcgtgac agcgacgacg tctcgacatt cacgctgtct ccggctgcga
     2941 tcttgacggt gccttcgagc atcgcgtcgc gtgtgcgacc gcgcagcagg tacgtgcccg
     3001 ctcgcaagct gagcgagcgc tgcgttgcgc ccgcggcgac ctcgccgaca acagccttcg
     3061 cgggctcggt gcgaaacacg agccacgtcg cgtccgcagg gagcgtgacc gtgccgcgct
     3121 cgctatcctt cgcgagatcc gcgagagcga cgtcggaccg tcctcggagg tcgtaatgaa
     3181 acgtcggatg ctggatgccc gaaagcgtgc ggctcgacgc gacgatcgtc tggtcacgcg
     3241 tgaaacgaaa cgcttccgcg accgtgacga tgtgatcgcc gttgtcgtca gcggcgccgc
     3301 gcatcgcgga caccaggtag tgcgtgaaga acgagccgcc cagctcgtcg gactcctgcg
     3361 catcctcgcc gagcgtgctc gcggtcagca cgatcacgcc ttcgccgggc agatcatccg
     3421 cactgctgat cgcgagggcg ggcgccggct tccctccctt gacgcgagtg agcgagccag
     3481 accgacacgc atcgatgacg agcacgcgga cttgcgccga cgagccgcgc aacagcttct
     3541 cgaggtcggt gagccgcagc cgcgtgtcgc ccaggtgaag cgactcggcg tcgccatggc
     3601 ccgagtagta gacaaacagc atcgcgctgt ttcccttctg ctggcgcatg cggatgcgct
     3661 cgttcatcgc gatcaatgcg cgcgtgacct catcagcgga cttgccgcga aggacgacct
     3721 ggttctcgtc gggaatgccg ccgacctcac cgaggagctc ggcaaagcgc gcggcatcac
     3781 tctccgcaaa ccgcaagcgt tgctcgtcgg caagaccatc gttgtctccg acgatcagcg
     3841 cgaaacgatc gacatcagcg gaggcgatcg cgctcgcggt gagcacgcat agagctgcca
     3901 gcatgcgaat catagccagc ctccgagaaa caggtttgcg tcgagcaaga cgccgggttc
     3961 gtcgcagcaa tcgccgatga ggaagtgcga ctgcagcgcc agctcggcgc tcgcgaagag
     4021 tcgtccggag agcggacgcg tcacaccagc cgcggcaccg atcatcgggc ctgcgtggcg
     4081 tgagatcacg gcgggtgtgt catggaaggc gtgatcgctc tcgcgcgcgt cgaggtacga
     4141 accaccgaga ctgccgccga gatcgacgga gaagtgcggg agctcccagg cgcgcgtgat
     4201 gcgaagctct gcgaccagca gattcgcgac gccgccatcg aaccgatcgg gccagccatg
     4261 gacagcgctg atgcgtggcg acagcgtcac cgcgggaagg acgtacatga atccgccgac
     4321 gacgccggga agggcgagcg agcctggaag cgccatgacc gtggcaccgg caaagaaacc
     4381 cgcgacgctc ggcagcacgt cccctctgcc cttgcgcacg agccgcgcgt acatcgttcg
     4441 cgtcagcgag tcgctgtcga cgctcgtctc cttcgcggcg gcgaccttga cgctgccctc
     4501 gagcagcgcg tcacgcgtgc ggccttggac cgcatatgaa ccggcacgca ggctgagctt
     4561 gcgctgcttc gcgtcggcgc cgatctcggc aatgacggtc ttgctcggcg acgtccgcac
     4621 gacgagccac gtcgcgccgg gcgggacgac gagcgtgccg cgcgcgttat cgggcgcgag
     4681 acgcgagagc gtgaggtccg cgcggccgcg caggtcgtac tcgaacgttg gatgctgcgc
     4741 gcccgccgac gagcggctcg attcgaggat ggtctgctcg cgcgtgtatt cgaatgcctc
     4801 ggacgcggtc accacaccat cgctgttcgt gtcagccgcg ccgcgcattc ccgatacgag
     4861 gtagtgcgtg aagaacgagc cgccgagctt ctccgattcc tgcgcgtctt cgccgagcgt
     4921 gctcgccgta aagacgatca caccatcggc aggcaacgac tcgtcgacgt cgatcgcgcg
     4981 cttgctgcct ttggttcgcg tcagcgagcc cgaacgacat gcgtcgatga cgagcacgcg
     5041 aaccgacgca gccgaaccgc gcacgagctg ctcgagctcg cgcagcgcga gcttcgtatc
     5101 gccgaggtgc agcgactcgg cgtcgccgtg ccccgagtag tagacgacca ggaccgagtc
     5161 gtgaccgctc gagcgaatgc gatcgttgat gtcgatcagc gcgcgacgca cttggtcggc
     5221 gttcttgcca cgcagtacca cttggttctc ggcggggacg cccccgatct cgtcgaggac
     5281 gtcggcgact tggctcgcgt ccttctcggc gaactgcagc cgctgctcgt cggccagacc
     5341 ttcgtcatgg ccgatcacga ccgcgaaccg ctcgacttcg gcgcgcgcag gagcggcgag
     5401 cccgcagaca accgcggcca tggccacgag cctcacggcg tcaccgcctg cttcgcgaga
     5461 tctatcgtcg acgccacgca gccttcgggc gccagcacag cgccggtccg cacagcctcg
     5521 atgaagggtg cgacctcgcg ttgagtggcg caccagatga tcacgacatg ctcggtgccg
     5581 agcacatcgt cgagctcggt gctctcggga aacacgtgct cgacgcctgc ggcaagcggc
     5641 acgagcagcg tcccggtcga cggcacgtat gcgttcgtgc tgccggcgcc atccttcgag
     5701 atgaccgcac cgaaaccggc cgtttcggcg ctgtacgacg cctgcagata cgtccccggc
     5761 gcgacgcgat cgccgctgcg gaccggcgcg aggtggcccg gcgcgccggc gaacaactgc
     5821 agcttcgcat cgtgcgtgcc tttgattcgg acgtcgtcct gttccgggcg cttcgcgagg
     5881 ccgaccacga cgagcgcgag cgcggccact acgaccggcg ctgcgtacag ccacgcgcgg
     5941 cgcttcgggc gggcggcgaa cgcgggcatc ggtcgcgcgg cgaatgcgtc gtgcccggct
     6001 ttcagcatgt gcagcctctc gccgcagacg ctgcacgact cggcatgcgc gcggagcgtc
     6061 gccgcatcgt cacgctcacc ggcgagcagc tgatcccacg cgaggtccga cgggtgatcg
     6121 ctcacgacgc ccgctcctct cccgcgaggc ggccgacgac gcgctccagc aagttgccga
     6181 cgtgtcgccg cgagcaaccg aggaggtctg cgatctcggc gtggctcatg ccgtcgaggt
     6241 agtaatagac cgcggtacgc gcctcgtcct cgctgagttg tgcgagcacg ctgcgcacga
     6301 cggtcgcggt ctccggggac cgccgatcca tctcggtcga ccacggccgc acatgctggt
     6361 cgatcaagcg gccgcggttc ttgcgatcac ggatgcgcgt gagacacgcg ttcgtcgtga
     6421 ccgcgtagag aaacgtgctc ggggagctgc gcccgtcgaa ctgccccggt cttgcgatga
     6481 gtcccatgaa gatctcctgg aggacctcgg cggcgtcgtc gtcgctgacg agcatctggc
     6541 gagcacggcg caacacgctg tgcccgtacg cccgatacat cgcctcgaca tcgcttgcgg
     6601 tcactcatct ccttgggagc gcgagggggc cgagagtggc acggcccaga agaaatcgcg
     6661 taagtgcctg ctatcctggg gtgtgctcga ccggcttgtc acggcgatcg gccagcggtt
     6721 cccgcaggcg acgtgtgtcg tcgacgctgg cgcggtgcgc atcgagctcg cgatcgacgg
     6781 cgtgacgtat cgcgcacgag ctcagatgga gccgcgaggc cgcgtcgtcg tccagctgcc
     6841 cagcacg
//
LOCUS       DATLBZ010000599        14244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10117056, whole genome shotgun sequence.
ACCESSION   DATLBZ010000599 DATLBZ010000000
VERSION     DATLBZ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14244
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10117056"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VMZ53_29405"
     CDS             <1..417
                     /locus_tag="VMZ53_29405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32668.1"
                     /translation="GKSCDKMKPPKGGVDAGVIPECEWHTPGILREIVAVEHAAAVRN
                     KVAFFDTFAAMGGGELMHRFVTSEPRIAFKDHIHLTTLGYEKWADALGGAVFEQYQRW
                     RKQNGLPPTKSLSPPPQTVPPPSTPPPEQLPGPVAP"
     gene            417..2798
                     /locus_tag="VMZ53_29410"
     CDS             417..2798
                     /locus_tag="VMZ53_29410"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HUS32669.1"
                     /translation="MLFSNPDYPIFLIAVFFMYALARGRDALGFWARAALLLALGDLV
                     FLLVAKDIDTLWDPIGGWLWRTATVGGHDEAWRHWTPYTVLVKWPIGLLVVGGSIYGG
                     MRSGPWLTSEKGQRTVARGIVIALAVVGAVVAFAWWQSALDGVTRVIAAHSHLLVLSV
                     LGVGIGLSRDETHRPLSRVITLFVASSLFYQAWAIAMPGPYRYLLGLLLGTIILDYYL
                     AIWIERSEDPAKRKLLVVVSLVSNLGILFFFKYADFVTQDVLQLPVSRLNLILPAGIS
                     FHTFQSLSYTIDVYRKELKATRSPIEFATFVLFFPQLVAGPIVRAQDLLPQLHQLPGM
                     EVQRATRGLYRIVVGLFKKIALADTFALFIADRVFENPTRFSSLEVLVGVYAYALQIY
                     CDFSAYSDIAIGSADVLGFKLPENFRTPYRSANLQEFWRRWHISLSTWLRDYLYITLG
                     GSRGAEWRTYLNLALTMVLGGLWHGASWAFIVWGGLHGFGLAVTRYFQRATAVDAKAA
                     WQLFAGCAGFFAVGYTVQTQVFPSAMGTWTNLVIAWLYATPLWAVLTAWLSREPSNAR
                     ARAAQPDAHELDVREPDGRVLGTPVPLGIAPWQSRIPQARLRDRGVLTLVPELLRFAM
                     CGAGALFLAALEWGEQWTWLPLIALTWALALAADIVERGPAPRLHDSRLVDFLLKRLA
                     VARRAVAVILVFHYVCFAWIFFRAGSFDTALAVLEQIAKLELDHANIVPVLTTALAVG
                     FACHFFAEGSFRWLRERWAMLPWYVQGTLLAAVALVLRELAHPKVVPIIYFQF"
     gene            2798..3553
                     /locus_tag="VMZ53_29415"
     CDS             2798..3553
                     /locus_tag="VMZ53_29415"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS32670.1"
                     /translation="MRNVLLLTLLAACGGTQPPPPPPPPQPSIDAELTPAFTKVQPWL
                     GHWRSDDGSTTHWTAADGAMYGVWFHDRDFNVMVVDDGEGPRPPDGVLRYIAIPSGQS
                     STERKDLTAAELAVFPHIAGYRAMKIERGPDDAAVEAADRAFAADVKARGADGWLAAF
                     DEDGGMLRKTGRIEGAAIREAMADTLANGLLAWDPIASGAEGELGYTVGKATYTPNTA
                     GEPGWASTYITIWRRQPDGSWKVLFDTGRAVNR"
     gene            complement(3640..6915)
                     /locus_tag="VMZ53_29420"
     CDS             complement(3640..6915)
                     /locus_tag="VMZ53_29420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32671.1"
                     /translation="MNRRAFFLGAAMVLLGSFVAIALRAYAEAAFLGAYGAKRLPWLL
                     IANAGGFAFATLGYDFVSRRARAGAIDLVLLIALIGAAAAAPSLLRSGVTPIVLVIAL
                     AAGSQVAGLALWNRVCAAVAGRDARRFLPRAGAGVTAGGAIAGLGAGVLIPRLGLSAI
                     PLIGAAVTAVVAAICVAQERALAKGGAPGGVPGGPGMPAVTSAGPVIAGIADVVAAKA
                     DAAKAALASAKAADPMSASASVSGTKAADAMSASASTSGAKPAASGLLAAGGLTPVQR
                     RLITAMFIVAGIEGVVATVIDLQFISAVKARWSGEDLAVALALFYGGTNLFLLLLQVA
                     AVPHLLVTRSLPFTAAIHPVIVILSYAGFAASPGFVGIAGTRTVDQVLRFATSRTSQE
                     VSLSALPPGPRARWKVLLRGAVWPAGAAVAALVLLLVGPPALRDPTRLAVIAIGVAIA
                     WAIAARVAARRFQTALAAPLGIRETRVDDPRRIDLETLERWAQAAGADDAKQAALARA
                     ALVRARIDATDLADHLRHDEPAVRAALFDQLARSPTPALRGELKAAIGIEDDDRALVL
                     GIKALAIAGDDAGIVRGEARAGLARDVDDAVASAKAMLRGGTTTSDATESDTTARDQL
                     GDELLKLLARDPAWAVAMVRARPGIFVDDFLDTKLREALANPATRAGAYAIIARVGPD
                     TTRPLLAAALEDGDAAALAAIEDIDLQGAASLAPSITSFSPLARATLARTLAAAPTTA
                     SELVAHLIVDGDPEVAYAALRTALAITRGGGTLPTIAVEAAYDNALAAFTAHLNARDA
                     VAAIAKPADVTWTACARTELEIATRRCAARVLWAAAVEAANAGRDPAPIAASARHLIG
                     SREADRKRALDVVQELQARPQLLALIERWLQPPKPSHDESALTALGTFDPWLAALGAG
                     ETRALEPMLVDLRRPALFSSIAGPALAALAERTTRIRVTGELFREGELGDTMYIVAAG
                     ALIAKRASAPTSISGERRIGVGEVVGELAVLTHAPRAATLSAATDDKHTAAVTDDKHT
                     AAVTDDKHTAAVTDDKRVAGTEVVAIDRAAFAAASRRAPELVLGLSSTLAGWLAPNRP
                     DVL"
     gene            complement(6912..7349)
                     /locus_tag="VMZ53_29425"
     CDS             complement(6912..7349)
                     /locus_tag="VMZ53_29425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32672.1"
                     /translation="MDLLERARTLAACPLFESLAPAVVIRLAERARPTELERGERRTS
                     DETVWVIASGSLAVMVRAPGDKLSDITASIARKRGANAVAGNAVGMIRVVRPSTPGVE
                     LIAEQPSTLLGLAIDDVRDVLEEDPAALTALADRLAALLLGAT"
     gene            7566..8003
                     /locus_tag="VMZ53_29430"
     CDS             7566..8003
                     /locus_tag="VMZ53_29430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32673.1"
                     /translation="MRTFLLACLVTSGCLVVASGCVVGDGGEPGSGDDQTEGGSTGGT
                     GSGTGSGTGMGTGTGTGMGTGSGSGSGTSAATCTNAVYDPCTNASQCTSGKCQLFNQQ
                     GIQVCTQTCTPGDNTTCPTQNGQAATCNNMGICKPPAANSCTR"
     gene            8113..8496
                     /locus_tag="VMZ53_29435"
     CDS             8113..8496
                     /locus_tag="VMZ53_29435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32674.1"
                     /translation="MSFVIALAAVSPAFAQGDKQEPKQAPAEVTPNEARQWMQLFDKV
                     VDTVVANKEDCGKMATGLNAVVDANQDAIAIARDAKTKGKKLPQTVQQHMIDGARRML
                     GALDKCGRDEKVGAAFRRIDLGGRK"
     gene            8683..8943
                     /locus_tag="VMZ53_29440"
     CDS             8683..8943
                     /locus_tag="VMZ53_29440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32675.1"
                     /translation="MMKVVDPSLIVPVPGKRHLLILDRPEGGLAGAAVLDIDGTRGHV
                     SALAIAPEYEGTHLEDRFIAVMEAMCEAFGVRSLDIPARRAA"
     gene            8964..9902
                     /locus_tag="VMZ53_29445"
     CDS             8964..9902
                     /locus_tag="VMZ53_29445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS32676.1"
                     /translation="MIEARVDVVIVGGGPAGLSAALVLGRCQRSVVVLDNKRYRNADT
                     RARVHGFITRDGTSPDEFRHLARADVARYPTVRIEEETVVDARRIERGFAVLTKSGRE
                     LQCGALLIATGFIDTVPTVAGARELHGQFVVPCPYCDAYEVRDQPLAAFSYPDDTGAH
                     YAYLLAQWSSDVVFCAERRPQLSDEMRARLAERNVRVEDRELRSIEREGDGIRLVFSE
                     GESLSRRILFYHLGGRGASNLPEKLGARVDERGSVDVDRKQAAIGVRGLWVAGDATRD
                     VLQSIVAAGEGASAAVCINEFLCGGESHGKPRIPNV"
     gene            9874..10224
                     /locus_tag="VMZ53_29450"
     CDS             9874..10224
                     /locus_tag="VMZ53_29450"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HUS32677.1"
                     /translation="MANRVSQTYDDITRRTVPDLDSSTRPSKDQERQAFEGYRAMDEH
                     ETALHARVMDALRNSGVNWQGVTVEVERDRVSVRGNVDEDHDLRRIPEIIRGVDGVAS
                     VEDRLVVLPGGAAD"
     gene            complement(10225..10671)
                     /locus_tag="VMZ53_29455"
     CDS             complement(10225..10671)
                     /locus_tag="VMZ53_29455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32678.1"
                     /translation="MTRSGPHPLGTPNGLSPNTGPLASAAWLVARGLSPSGPKWFVEM
                     ELDAAGNSERAPAAKLLLEVYAEEWGYRFERDGRISWIRVTDVPFVHGRDEHGLLPKT
                     PRLASIGELMRELEAAHAITFERHRPTIRSNVPDGEKAIPLWIASL"
     gene            10862..11473
                     /locus_tag="VMZ53_29460"
     CDS             10862..11473
                     /locus_tag="VMZ53_29460"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HUS32679.1"
                     /translation="MESAATSLLRLNLETQSHHAMADQLWAPLLAPDVTKWQYVARLA
                     LVYGFEAPLESALAYTPNLRLAIDIRSRIRAGRVAQDLITLGLSAGEVADLPQCGQMA
                     PFSSPLEGLGWLYVSERSTLQHDAVRKNVVGKMPDIANATAYLSVYDGIVGLRWHELG
                     AAFDRSVRTERALEEVIAGAQAGFRCLASWSGVTAQMQSRAKR"
     gene            complement(11477..11725)
                     /locus_tag="VMZ53_29465"
     CDS             complement(11477..11725)
                     /locus_tag="VMZ53_29465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32680.1"
                     /translation="MRTSLRAAGLSVRLTRVDFPAALHAALTWGSFDAVVFDPETPTI
                     SLEMVTAALRERNISAPIIVMSGDTGKDVVAQLRARRN"
     gene            11933..12400
                     /locus_tag="VMZ53_29470"
     CDS             11933..12400
                     /locus_tag="VMZ53_29470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32681.1"
                     /translation="MPGRRAGVQLAGRSQTSSAGTVAANAWLTALGLNPPPGLLRWHA
                     EISLDVIDGPASAEYDETRDSRFHIDIYSEEWGFFFCHGGRVSWIRVTDIPFVHGRDD
                     HQLLMQTPALPDVGKLMRALETKHTLRFNRPQALVRTNIVAAEPMIRRWLQTL"
     gene            12451..13284
                     /locus_tag="VMZ53_29475"
     CDS             12451..13284
                     /locus_tag="VMZ53_29475"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS32682.1"
                     /translation="MGTATNRQFAVITGASSGIGFELAKQCVLNNFDILICAEDEGIH
                     EAARHLGATGVAVEAVHADLATFEGCEKLVTAVNSYGRPLDALLLNAGIGVGGAFVQN
                     ELEDELRMIALNVNAVVHLAKRLVPRMVTRGQGRVLVTSSVAATSPAPFLAVYGATKA
                     FDLSFAEALRFELKDTGVTVTALQPGATDTEFFDRADMDNTKVAQGKKDDPEDVAKKG
                     FDAMMAGKDKVIAAGLKSKLEGIAGELLPEPVKAKMQASQTRPGSAEKHTVRELNKKL
                     P"
     gene            13338..13571
                     /locus_tag="VMZ53_29480"
     CDS             13338..13571
                     /locus_tag="VMZ53_29480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32683.1"
                     /translation="MVTTRIARPTDLVFPLVDADEPDPIAEAEAAATELDWSFHDAEL
                     TQQLKAVHRGRGWLLLGLTSSVLAIAAGVAIVI"
     gene            complement(13568..14143)
                     /locus_tag="VMZ53_29485"
     CDS             complement(13568..14143)
                     /locus_tag="VMZ53_29485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32684.1"
                     /translation="MALRRIVLALAVSAASACGGAAPNDPGFPVPPPPSTAPPIARAM
                     PAPVLSRFQQPITAPSSPKRESITEPPPGLPLPKVDEERVARQSPAEPRPPLPTDTLP
                     DEVVMRLLESGRAAFVRCFKKARNEDPSELSFKVKLRVELDADGSFSRASTDAHSAAL
                     DTCLTRMAAWLKFPAAGKPIAVELPLFYQAN"
BASE COUNT         2310 a         4699 c         4811 g         2424 t
ORIGIN      
        1 ggcaagagct gcgacaagat gaagccgccg aagggcggag tggatgccgg cgtgattccg
       61 gaatgcgagt ggcacacgcc gggcatcctg cgcgagatcg tcgcggtcga gcatgcggct
      121 gcggttcgca acaaggtcgc cttcttcgac acgttcgcag cgatgggcgg tggcgagctc
      181 atgcacaggt tcgtgacgag cgagccacgc atcgcgttca aagatcacat tcacctgacg
      241 acgctcggct acgagaagtg ggctgatgcg ctcggcggcg cggtgttcga gcagtaccag
      301 cgatggcgca agcagaacgg cctgccgccg acgaagtcgc tctcgccgcc gccacagacc
      361 gtgccgccac cgtcgacgcc accgcctgaa cagctccctg gtccggtcgc gccgtaatgc
      421 tgttctcgaa tcccgattac ccgatcttct tgatcgcggt cttcttcatg tatgcgctcg
      481 cgcgaggtcg cgatgcactc ggcttctggg cgcgcgcggc gctgctactc gcgctcggtg
      541 acctcgtctt cctgctcgtc gccaaagaca tcgacacgct gtgggatccg atcggcggct
      601 ggctctggcg cacagcgacg gtcggcggtc acgacgaagc gtggcggcat tggactccgt
      661 acaccgtgct cgtgaagtgg ccgatcggcc tgctcgtcgt cggcggatcg atctacggcg
      721 gcatgcgctc cgggccgtgg ctgacgagcg agaagggcca gcgcacagtc gcgcgcggca
      781 tcgtcatcgc gctcgcggtg gtcggcgcgg tcgtcgcgtt cgcatggtgg caaagcgcgc
      841 tcgacggcgt cacgcgcgtg atcgcggcgc actcgcacct cctggtgctc tcggttctcg
      901 gcgtcggtat cggcctatcg cgcgatgaga cacatcgccc cctgtcgcgc gtgatcacgc
      961 tcttcgtcgc gtcgtcgctg ttctatcaag cgtgggcgat cgcgatgccg ggcccgtatc
     1021 gctacctgct gggtctgctg ctcggcacga tcatcctcga ctactacctc gcgatttgga
     1081 tcgagcgctc cgaggatccc gcgaagcgca agctgctcgt cgtcgtctcg ctcgtctcga
     1141 atctcgggat cttgttcttc ttcaagtacg ccgatttcgt cacgcaggat gtgctgcagc
     1201 tgccggtgag ccggctgaat ttgatccttc ccgccggcat ctcgttccac acgtttcagt
     1261 cgctgtcgta cacgatcgat gtctatcgca aggagctcaa ggcgacgcgc tcgccgatcg
     1321 agttcgcgac gttcgtgctg ttcttcccgc agctcgtcgc aggtccgatc gtgcgcgcgc
     1381 aagacttgct gccgcagctc caccagcttc caggcatgga ggtccaacgc gcgacgcgcg
     1441 gcctctatcg catcgtcgtc ggtctgttca agaagatcgc gctcgccgat acgttcgctc
     1501 tctttatcgc cgaccgcgtg ttcgaaaatc cgactcgctt ctcgagcctc gaagtcctcg
     1561 tcggtgtgta cgcgtacgca ctgcagatct actgcgactt ctcggcgtac tccgatatcg
     1621 cgatcggttc ggcggatgtg ctcggcttca agctgcccga gaacttccgc acgccgtatc
     1681 gcagcgcgaa cctccaagag ttctggcggc gctggcacat ctcgctgtcg acgtggctgc
     1741 gcgactacct gtacatcacg ctcggcggta gtcgcggcgc cgagtggcgc acgtatctca
     1801 atctcgcgct gaccatggtg ctcggtggcc tctggcatgg cgcgagctgg gcgttcatcg
     1861 tgtggggcgg tctgcacggc ttcggcctcg cagtcacgcg ctacttccag cgagcgaccg
     1921 ccgtcgatgc gaaggccgcg tggcagctgt tcgccggatg cgccggcttc ttcgcggtcg
     1981 gctacaccgt gcagacgcag gtgttcccgt cggcgatggg aacgtggacg aacctcgtca
     2041 tcgcgtggct gtacgcgacg ccgctgtggg ccgtgctcac cgcgtggctc agtcgcgagc
     2101 cctccaacgc gcgcgcccgc gccgcgcaac ccgacgctca cgagctcgat gtgcgcgagc
     2161 cggacggtcg tgtcctcggc acgccggttc cgctcggcat cgcgccgtgg cagtcacgga
     2221 tcccgcaagc gcggcttcgt gatcgcggtg tgctgacgct cgtccccgag ctcctgcgct
     2281 ttgcgatgtg cggtgccggc gcgctgttcc tcgcggcgct cgagtggggc gagcagtgga
     2341 cctggcttcc gctgatcgcg ctcacgtggg cgctcgctct cgcggccgat atcgtcgagc
     2401 gtgggccggc gccgcgcctg cacgacagcc gcctcgtcga tttcttgctg aagcgcctgg
     2461 cagtcgcgcg ccgcgcggtc gctgtcatcc tcgtcttcca ctacgtgtgc ttcgcgtgga
     2521 tcttcttccg cgcaggctcg ttcgacacgg cgctcgcggt gctcgaacaa atcgcgaagc
     2581 tcgagctcga tcacgcgaac atcgtgccgg tgctcaccac ggcgctcgcg gtgggcttcg
     2641 cgtgtcactt cttcgcggaa ggcagcttcc gctggctgcg cgagcgctgg gcaatgctgc
     2701 cgtggtacgt gcaaggcacg ctgctcgcag cggtcgcgct cgtgctgcgc gagctggcgc
     2761 atcccaaagt cgtccccatc atctacttcc agttctaatg cgcaacgtcc tcctcctcac
     2821 cttgctcgcc gcgtgtggcg gcacacagcc gccgccgccg ccgcctccgc ctcagccttc
     2881 gatcgacgcc gagctcacgc ccgcgttcac gaaggtccag ccgtggctcg gtcactggcg
     2941 cagtgatgac gggtcgacga cgcactggac cgccgccgac ggcgcgatgt acggcgtgtg
     3001 gtttcacgac cgcgacttca acgtaatggt cgtcgatgac ggcgaaggcc cgcgaccgcc
     3061 cgacggcgtg ctgcgctaca tcgcgatccc cagcggccaa tcgtcgacgg agcgcaagga
     3121 tctcactgct gccgagcttg ccgtgttccc gcacatcgcg ggctatcgcg cgatgaagat
     3181 cgagcgcggc cccgacgacg ctgcggtcga agccgcggac cgcgccttcg ccgccgacgt
     3241 aaaggcgcgc ggcgcggacg gttggctcgc cgcgttcgac gaggacggcg gcatgttgcg
     3301 caagacaggc cgcatcgaag gcgccgcgat tcgcgaggcg atggccgaca cgctcgcgaa
     3361 cggtctgctc gcatgggatc caatcgcgag tggcgccgaa ggcgagctgg gctacacggt
     3421 cggcaaggcg acgtacacgc cgaacacggc aggcgagccc ggctgggcgt cgacgtacat
     3481 cacgatctgg cgccgccaac ccgacgggag ctggaaggtg ctgttcgata cgggccgcgc
     3541 cgtaaatcgc tagtccgccg tcgcgcgctc gcctcgccgt cgcgcgctcg cctcgccgtc
     3601 gcccgctcgc tcgctcgtcg cccgctgacc gcgagctgcc tagagcacgt cggggcgatt
     3661 gggcgcgagc cagccggcga gcgtcgacga aaggccgagg accagctcgg gcgcgcgccg
     3721 cgacgcggcc gcgaaggccg cgcgatcgat cgcgacgacc tccgtcccgg cgacgcgctt
     3781 gtcgtcggtc acggcggcag tgtgcttgtc gtcggtcacg gcggcagtgt gcttgtcgtc
     3841 ggtcacggcg gcagtgtgct tgtcgtcggt cgcggcgctg agcgtcgcgg cgcgcggcgc
     3901 gtgtgtcagt acggcgagct ctcccacgac ttcgccgacg ccaatccgac gttcgccgct
     3961 gatgcttgtc ggtgcgctgg ctcgttttgc gatgagcgcg ccggcggcga cgatgtacat
     4021 cgtgtcgcct agctcgcctt cgcggaagag ctcgccggtg acgcggatcc gcgtcgtgcg
     4081 ttcggcgagg gcggcgaggg cggggccggc gatcgacgag aagagggcgg ggcgccgcag
     4141 atcgacgagc atcggctcga gcgcgcgtgt ctcgcccgcg ccgagcgccg cgagccatgg
     4201 atcgaatgtg ccgagggcgg tgagcgccga ttcgtcgtgc gatggctttg ggggctgcag
     4261 ccagcgttcg atcaacgcga gcagctgtgg gcgtgcttgt agctcttgca cgacatcgag
     4321 cgcgcgtttg cgatcggctt cgcggctacc aatgagatgg cgagcgctcg ccgcgatggg
     4381 ggcgggatcg cgaccagcgt tcgcggcttc gacggccgcg gcccagagca cgcgcgctgc
     4441 gcaccgccgc gtggcgatct cgagctcggt gcgcgcgcac gcggtccacg tcacgtcggc
     4501 gggcttggcg atcgcggcga cagcgtcgcg cgcgttgagg tgcgcggtga acgccgccag
     4561 cgcgttgtcg tacgcagcct cgacggcgat ggtcgggagg gttccgccgc cgcgcgtgat
     4621 cgcgagcgcg gtgcgcaggg cggcgtacgc gacctcgggg tcaccgtcga cgatgaggtg
     4681 cgcgacgagc tcgctggctg tcgtcggagc ggcggccagt gtgcgcgcga gggttgcgcg
     4741 ggcgagcggc gagaagctcg tgatcgatgg tgcgagcgac gcggcacctt ggagatcgat
     4801 gtcttcgatt gcggcgagtg ctgcagcgtc gccatcttcg agcgcggctg ctagcagtgg
     4861 tctggtggtg tccggaccta cgcgcgcgat gatcgcgtag gcgccggcgc gcgttgcggg
     4921 gttcgcgagc gcttcgcgga gcttggtgtc gagaaaatcg tcgacgaaaa tgcctggcct
     4981 cgcgcggacc atggcgaccg cccatgccgg atcgcgcgcg agcaacttca ggagctcgtc
     5041 gccgagttgg tcacgggccg tcgtgtcgct ctcggtcgca tcgctcgtgg tcgtgccccc
     5101 tcgcagcatc gcctttgcgg atgcgacggc gtcgtcgacg tcgcgcgcga ggccggcacg
     5161 cgcctcaccg cgcacgatgc ccgcatcgtc gcctgcgatc gcgagcgcct tgatgccgag
     5221 cacgagagcg cggtcgtcgt cttcgatccc gatcgcggcc ttgagctcgc cgcggagggc
     5281 gggcgtcggc gagcgggcga gctgatcgaa taaggcggcg cggaccgcgg gctcgtcgtg
     5341 gcgcaggtga tctgcgaggt cggtcgcgtc gatacgcgcg cggacgagcg ctgcgcgcgc
     5401 gagcgctgct tgcttggcgt cgtctgcgcc agcagcttgc gcccagcgct cgagcgtctc
     5461 gagatcgatt cgtcgcggat cgtcgacacg cgtctcgcga atgccgagcg gtgcagccaa
     5521 cgccgtctgg aaccgacggg cggcgacgcg tgcggcaatc gcccacgcaa ttgcgacacc
     5581 gatcgcgatc actgcgagtc gcgtcggatc gcgcagcgcc ggcggaccga cgagcaacag
     5641 cacgagcgcg gcgaccgctg cacccgcggg ccacaccgcg ccgcgcagca gcaccttcca
     5701 tcgcgcacgc ggcccgggcg gcaacgcgct cagcgaaacc tcctgcgagg tccgcgacgt
     5761 cgcaaagcgc agcacctgat cgacagttcg cgtcccggca atcccgacga agccgggcga
     5821 cgccgcgaat cccgcgtacg agaggatcac gatcaccgga tgaatcgccg cggtgaacgg
     5881 caagctgcgc gtcacgagca agtgcgggac ggccgcaacc tgcagcaaca gcaagaacag
     5941 attcgtgccg ccgtaaaaca gtgcgagcgc cacggccaag tcttcgccgc tccaccgcgc
     6001 cttcaccgcc gagatgaact gcaggtcgat caccgtcgcg accacgcctt cgatccctgc
     6061 aacgatgaac atcgcggtga tgaggcggcg ctgcacaggc gtgagcccgc cagcggcgag
     6121 taagcccgag gccgccggct tcgcgccgga cgtgctcgcg cttgcggaca tcgcatccgc
     6181 ggccttcgtg cccgatacgc tcgcgctcgc ggacatcgga tccgctgctt tcgcgcttgc
     6241 tagcgcggct ttggctgcgt cggctttcgc ggcgacgacg tcggcaatgc ctgcgatgac
     6301 cgggccggcg ctcgtcaccg cgggcatgcc tggcccgccg ggaacgccgc ccggcgcgcc
     6361 gccctttgcg agcgcgcgct cctgggcgac gcagatcgcc gcgacgaccg ccgtcacggc
     6421 tgcgccgatc agcggaatcg ccgacaagcc gagccgcggg atgagcacgc ctgcgccgag
     6481 gcccgcgatc gcgccacccg cggtgacgcc tgcaccggcg cgcggcaaga acctcctcgc
     6541 atcacgcccg gccaccgccg cgcaaacgcg attccacagc gcgaggcccg cgacctgcga
     6601 gcccgccgct aatgcgatga cgagcacgat cggcgtaacg cccgatcgga gcagcgacgg
     6661 tgccgccgcc gcagcgccga ttagcgcgat gaggagcacg agatcgatcg cgcccgcgcg
     6721 cgcccgccgg cttacgaagt cgtagcccag cgtcgcgaac gcgaagccgc ccgcatttgc
     6781 gatcaggagc cacggcaatc gcttcgcgcc gtatgcgccg aggaatgcgg cctcggcata
     6841 tgcgcgcagc gcgatcgcga cgaagctgcc gagcagcacc attgcggcac cgaggaagaa
     6901 cgcgcgccgg ttcatgttgc accgaggagc agcgccgcca gacggtcggc gagggcggtg
     6961 agcgcggctg ggtcttcttc gaggacgtcg cgaacatcgt cgatcgcgag gccgagcagc
     7021 gtgctgggtt gttcggcgat cagctcgacg cctggcgtcg atgggcggac gacgcggatc
     7081 atgccgacag cgttgcccgc gactgcattc gcgccgcgct tgcgcgcgat cgacgccgtg
     7141 atgtcggaga gcttgtcgcc cggggcccgc accatcacgg cgagcgagcc cgatgcgatg
     7201 acccagacgg tctcgtcgga tgtgcggcgt tcgccgcgtt cgagctcggt cggacgcgcg
     7261 cgttcggcga ggcgaatgac aaccgctggc gccagcgact cgaacagcgg acaggctgcc
     7321 agtgttcgcg cgcgctcgag gaggtccatc gtcgtggtca gcgcggcgaa acgcgcgtgt
     7381 actgcgtgta aggcaactcg cgtttgcgcg taacggccaa atcctagccg ggactctccc
     7441 ccggagggtg gggagcgacg cggattcgtt caggttcgcg gcgacgaggc gatgctgggt
     7501 tgcgcggctg aaaatgacgg cgcgatgaca gcgagctggc gcgggcgctg cactttgccg
     7561 cgtgcatgcg gacgttcctc ctcgcgtgtc tcgtgacctc cggctgcttg gttgtcgctt
     7621 cgggctgcgt ggtcggcgac ggcggcgagc caggaagcgg cgacgatcag accgaaggtg
     7681 gcagcacggg tggcacagga agcggcactg gcagcgggac gggcatggga accggcaccg
     7741 gcacgggcat gggcaccggt agcggttcgg gcagcgggac atcggcggcg acctgtacga
     7801 acgctgtcta cgatccctgc actaatgcgt cgcagtgcac gtcgggcaaa tgccagctct
     7861 tcaatcagca gggcatccag gtctgcacgc agacgtgtac acccggcgac aacacgacgt
     7921 gcccgacgca gaacggccag gcggcgacct gcaacaacat gggcatctgc aagccgcccg
     7981 cagcgaacag ctgcacgcgc tagggcattt cccgatcgta aaactgcacg cggtcagaac
     8041 tctgcggacg cgcgcgcgct cgcgacatgt catcgtcgct gccctggggg cacgctttcg
     8101 atgaagctcg tcatgtcgtt tgtgatcgca ctggccgctg tttcgcctgc attcgcgcaa
     8161 ggcgataagc aggaaccgaa gcaagcaccc gctgaagtca cgcccaatga ggcgcgtcag
     8221 tggatgcaac tgttcgacaa ggtcgtcgac accgtcgtcg cgaacaaaga agactgcggc
     8281 aagatggcga ccggcttgaa tgcggtcgtc gacgcgaatc aggatgcgat cgcgatcgcg
     8341 cgcgacgcga aaacaaaggg caagaagctg ccgcagacgg tgcagcagca catgatcgac
     8401 ggcgcgcgcc gcatgctcgg tgcactcgac aagtgtggcc gcgacgagaa ggtcggagcg
     8461 gcgtttcgtc gcatcgatct cggcggccgc aagtaatccc cgctggtcgt tcttcgcatc
     8521 atcgtctgaa tctagatcga cgtcgtcgag cacacggcgt ttggagcagc gcgcgcgata
     8581 gcgcgggagt gccgcggcac ctcgcgtgca ctatcatcca tcatgatttc gctctcgacg
     8641 attcgaagcg gttccgtgga ggacatcgct ggcgtcagac gcttgatgaa ggtagtcgat
     8701 ccatcgctga tcgtgcccgt gccgggcaag cggcacttgc tgatcctcga tcgacccgaa
     8761 ggtgggcttg caggcgccgc ggtgctcgac atcgatggaa cgcgcgggca cgtctcggca
     8821 ctcgccatcg cgcccgagta cgaaggtacg cacctcgaag accgcttcat cgcggtgatg
     8881 gaagcgatgt gcgaggcctt cggtgtgcgc tcgttggaca tccctgcgcg ccgcgcggcc
     8941 tagcgcgtgc atcaagtgat tccatgatcg aggcgcgcgt cgacgtggtg atcgtgggag
     9001 gggggccggc gggtctctcg gccgcgctcg tcctcggtcg gtgccagcgc tcggtcgtgg
     9061 ttctcgacaa caagcgctat cgcaacgccg atacgcgcgc gcgagtgcac ggcttcatca
     9121 cgcgcgacgg tacgagcccc gacgagtttc gtcacctggc tcgcgccgac gtcgcgcgct
     9181 atccgacggt gcgcatcgag gaggagactg tcgtcgatgc gcggcgaatc gaacgcggct
     9241 ttgcggtcct gacgaagtca ggcagggagc tgcagtgcgg tgcgctcctg atcgccacgg
     9301 gtttcatcga tacggtgccc acggttgccg gtgcgcgcga gctccacggg cagttcgtcg
     9361 ttccgtgccc gtactgcgac gcgtatgaag tccgcgatca gccgctcgcc gcgttctcgt
     9421 atcccgacga tacgggcgcg cactacgcct acttgctcgc gcaatggagc agcgacgtcg
     9481 tgttctgcgc agagcgtcgc ccccagctct ccgacgaaat gcgcgcgcgt cttgctgaac
     9541 gcaatgtgcg agttgaagat cgcgagctgc ggtccatcga gcgcgaaggc gacggcattc
     9601 gcctcgtttt ctccgaggga gaatcgctgt cgcgacggat cctcttctat cacctcggtg
     9661 gccgtggagc gtcgaacctt cccgagaagc tcggcgcgcg cgtcgatgaa cgcggcagtg
     9721 tggacgtcga tcggaagcaa gccgcgatcg gtgtacgcgg gctgtgggtc gccggcgacg
     9781 ccacgcgaga cgttttgcaa tcgatcgtcg cggcgggcga gggcgcatcc gcagctgtct
     9841 gtatcaacga gttcctttgt ggaggcgaat cacatggcaa accgcgtatc ccaaacgtat
     9901 gacgacatca cccggcgcac cgtgccggat ctcgacagct ccactcggcc gtcgaaggat
     9961 caagagcgcc aagcgttcga aggctatcgc gcgatggacg agcacgagac tgcgctgcac
    10021 gcgcgcgtga tggacgcgct tcgcaacagc ggcgtgaact ggcagggcgt caccgtcgag
    10081 gtcgagcgcg accgcgtatc cgttcgcggc aacgtcgatg aagatcatga tctgcggcgt
    10141 atcccggaga tcattcgcgg cgtcgatggc gtcgcgagcg tcgaagatcg actcgtcgta
    10201 ctccccggcg gcgcagcaga ttagctacag cgaggcgatc cacaacggga tcgccttctc
    10261 gccatcgggc acgttggaac ggatcgtcgg acgatgccgc tcgaacgtga tcgcgtgcgc
    10321 cgcttcgagc tcgcgcatca gctcaccgat cgatgcgagt cgaggtgtct tcggaaggag
    10381 gccatgctcg tcgcggccgt ggacgaacgg cacgtcggtc acgcgaatcc acgagatgcg
    10441 gccgtcgcgc tcgaagcgat agccccactc ttcggcgtag acctcgagaa gaagcttcgc
    10501 cgccggcgca cgttcggaat ttcctgcggc gtcgagctcc atctcgacga accacttcgg
    10561 gccagacggc gagagaccgc gcgctacaag ccatgcagcg gacgcgaggg ggccggtatt
    10621 cggcgatagg ccgtttggtg tgccgagcgg gtgcggccca gaacgcgtca cggttctcat
    10681 acgttagtcg ctcccgttac ccgacgacaa cccggaaagt ttacgcgccg cgtgctgtgc
    10741 gagtgatgct cgttgcgctt ttgcgtgtgt ggcaacagcg cgcggttttg acgcggcaac
    10801 caaaacaccg acgccaccga atacctagca tcggttttgc tactacccgg gcggtacccc
    10861 aatggagtcg gcagcgacga gcctgcttcg tctcaacctc gagacgcagt cgcaccacgc
    10921 aatggccgat cagctttggg cgccactcct cgcacccgac gttacgaagt ggcagtacgt
    10981 cgcgcgcctc gcactcgtgt acgggttcga agctccgctc gagtccgcgc tcgcgtacac
    11041 gccgaatctt cgactcgcga tcgacattcg ctcgcgcatt cgcgcaggtc gtgttgcaca
    11101 agatttgatc acgctgggtc tctctgccgg cgaggtcgct gatctcccgc agtgcggcca
    11161 gatggcaccc tttagcagcc cgctcgaagg cctcgggtgg ctctacgtca gcgagcgctc
    11221 gacgctgcag catgatgccg tccgcaagaa cgtcgtcggt aaaatgcccg acatcgccaa
    11281 cgcgaccgcg tatctgtcgg tctacgacgg aatcgtcggc ctgcgctggc acgagctcgg
    11341 cgctgcgttc gaccgctccg ttcgcacgga gcgcgcactc gaggaagtga tcgctggagc
    11401 acaagcgggc ttccgttgtc tcgcgagctg gtccggcgtc accgcgcaga tgcaatcgcg
    11461 cgcgaagcgt tagtcgtcag ttgcgacgcg cgcggagttg cgcgaccacg tctttgccgg
    11521 tgtcgccgct catcacgatg atcggtgcgc tgatgtttcg ttcgcgcagc gcagcggtca
    11581 ccatctcgag gctgatggtc ggcgtctcgg gatcgaatac gacggcgtcg aacgagcccc
    11641 acgtcagcgc cgcgtgcagc gccgcgggaa agtcgacgcg cgtcaggcgc acgctgagcc
    11701 ccgccgcacg cagagatgtg cgcacgcgct cggcgtccaa cgtccaaagt cgaacaagca
    11761 atatgttcaa cggagggctc acgtgcggca attcctcgtt ttgcagcctc cgagccattt
    11821 tctgacctgt cgctagatcg cacacgcgcg aagacgacac acttgggcgg cgcctgtcag
    11881 aatggggaga atgcgtgtgg ggctcccacg tggcgtggta gaaatttcgc ctatgcccgg
    11941 acgacgggcc ggcgttcagc tggcgggcag aagtcaaacc tcgagtgccg gaacggtcgc
    12001 ggccaacgcg tggctgaccg cgctgggcct caacccgcct ccaggcctcc tgcgctggca
    12061 cgcggaaatc tcgctcgacg tgatcgacgg tcccgcgagc gctgagtacg acgagacgcg
    12121 cgactcgcgt ttccacatcg acatctactc cgaggagtgg ggtttcttct tttgtcacgg
    12181 tggtcgggtg tcctggattc gcgttaccga catcccgttc gtgcacggcc gcgacgatca
    12241 tcagctgctc atgcagacgc cggctcttcc tgacgtcggg aagctgatgc gcgcactaga
    12301 gacaaagcac accctccgtt tcaatcgccc acaggcactc gtgcgcacga acatcgtcgc
    12361 tgcagagccg atgatccgtc gctggttgca gacgctctag ctggtacgtc gctcgcaacc
    12421 tcctcggtta acaacaaacc cgagggagtc atgggcaccg caaccaatcg tcagttcgcc
    12481 gtcatcaccg gcgcgtcgtc gggaatcggc ttcgagctcg caaagcaatg cgtgctgaac
    12541 aacttcgaca tcctgatctg cgcagaggat gagggaatcc acgaggccgc gcgtcacctc
    12601 ggtgcaacag gtgtcgccgt cgaagctgtc cacgccgatc tcgcaacgtt cgagggctgc
    12661 gagaagctcg tcacggcggt caacagctac ggccgtccgc tcgacgcgct tttgctcaac
    12721 gccggcatcg gcgttggtgg cgcgtttgtc cagaacgagc tcgaagacga gctgcgcatg
    12781 atcgctctca acgtgaacgc agtcgttcac ctcgcgaagc ggcttgttcc gcgcatggta
    12841 actcgaggcc aaggtcgcgt gctcgtcacc tcatccgtcg ccgcgacatc tcccgctccg
    12901 ttcctcgcgg tctacggcgc gacgaaagcg tttgacctgt cgtttgcgga agcgcttcgc
    12961 ttcgagctca aagatacggg cgtcaccgtc accgctcttc agccgggcgc gacggacacc
    13021 gagttcttcg atcgcgccga catggacaac acgaaagtcg cgcaaggcaa gaaggacgat
    13081 ccggaggacg tcgccaagaa gggcttcgac gccatgatgg ccggcaagga caaggtcatc
    13141 gcagctggcc tcaagagcaa gctcgagggc atcgcgggcg agctgctgcc ggagccggta
    13201 aaggcgaaga tgcaagcgag ccaaacgcgg ccaggttccg ctgagaagca cacggttcgc
    13261 gagctcaaca agaagctgcc ctgagcagcg gcgctgtcgc acatacacat ccacgcggtg
    13321 cggccgaccc atcgagcatg gtgaccaccc gcatcgcgcg tccgactgac ctcgtgttcc
    13381 cgctcgtcga tgcggatgag cccgatccga tcgccgaggc ggaggctgct gcgaccgagc
    13441 tcgactggag cttccacgat gccgagctaa cgcagcagct caaggccgta catcgcggtc
    13501 gcggttggtt actgctcggc ctcacgtcga gcgtcctcgc gatcgccgcc ggcgtcgcga
    13561 tcgttatcta gttcgcttga tagaacagcg gcagctcgac ggcgatcggt ttgcccgccg
    13621 caggaaactt cagccacgcc gccatgcgcg tgaggcacgt gtcgagcgct gcgctgtgcg
    13681 cgtcggtcga tgcgcgcgag aacgagccat cggcatcgag ctcgacacgc agcttcactt
    13741 tgaacgacag ctcgctcgga tcttcattgc gcgccttctt gaagcagcgc acgaatgcgg
    13801 cgcggccgct ctcgagcaga cgcatcacga cttcatcggg cagcgtgtcg gtcggcaacg
    13861 gtgggcgagg ctcggccggc gattgacgcg ccacgcgttc ttcatcgacc ttcggtagcg
    13921 gaagaccagg cggcggctcc gtgatcgact cgcgtttcgg cgacgaaggt gctgtgatcg
    13981 gctgctggaa gcgagaaagc accggcgcgg gcatggcacg ggcgatcggc ggtgcggtcg
    14041 acggcggtgg cggaaccggg aagcccggat cgttcggcgc agcgccgcca cacgctgacg
    14101 ctgcactgac ggcgagcgcc aggacgatcc tccgaagggc cacatagaac tgtttccctg
    14161 agtggggcaa acgttcaagt tcttgttggt gagcgctcgg gcaggcgtcg gtaatcgacg
    14221 atctcccggc cggcaggtgg gtgt
//
LOCUS       DATLBZ010000600         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10117607, whole genome shotgun sequence.
ACCESSION   DATLBZ010000600 DATLBZ010000000
VERSION     DATLBZ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10117607"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..671
                     /locus_tag="VMZ53_29490"
     CDS             132..671
                     /locus_tag="VMZ53_29490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS32685.1"
                     /translation="MQMQASLLARVAAGDASAVRECIAQYGGLVWSIARRFELADAED
                     AVQEIFLDLWKSAARFDPKIASEAAFIAMIARRRLIDRRRTRRRRPSTEQLPEVPTHA
                     DEASLPDTCAEAKQAARALDQLRPEQRQVLVLSTCHGMSHGEIAAETGMPLGTVKAHA
                     RRGLLSIRAALLGVQGGES"
     gene            668..1654
                     /locus_tag="VMZ53_29495"
     CDS             668..1654
                     /locus_tag="VMZ53_29495"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HUS32686.1"
                     /translation="MKPALSPRAEELLAERAVRPLDDRELHELDALDASNDDSFDLAA
                     ASVAVALTPLETMPADLAAKILAASPAGFDAMKTVPGTSLMPGAIRTLPGVGLDAYRP
                     QPDAQVVAREGEPLVASRGDVPVAPQGDVRVAPQGDALGAPKSDIERAREVRAKRSRF
                     AVVAPWLAAAACLLAAVGALAWARDQGSGTKTVATLSPSDARADLLAHASDVTTIEWT
                     TTADPTAQGAKGDVVWSQSAQKGFMRFAGLAVNDAKQFQYQLWIFDKTRDEAFPVDGG
                     VFDVTASGEVIIPISAKLPVGDATLFAVTIEKPGGVVVSKRERIVVTAARKS"
     gene            1711..2607
                     /locus_tag="VMZ53_29500"
     CDS             1711..2607
                     /locus_tag="VMZ53_29500"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HUS32687.1"
                     /translation="MTASAISGAADNAIHEAKPWLEKLARAGFVAKGLLYMTIGALAT
                     AAALGLGRSTTGQMSAAGHAGPASTMGQRGAMAALLQAPAGRALLIAIAVGLFGYAAW
                     RFIEAIRDPMHHARSENKKKAKGILKRLRSAALGIIQVALGISALKIAFGNIEAANDG
                     KASAHWTGKALATPGGELVLWAIAGGLVAYGVYQLYKAWASKLSKELMLGRLSSGARR
                     LVIGASRFGIAARGIVFATTGILVGRAILQRDPQQVKGIKASLIELLELGRWPFAIVA
                     MGLIAYGIYQLINARYRRIEIA"
BASE COUNT          427 a          896 c          927 g          440 t
ORIGIN      
        1 gcattcgcga tcggcggcaa gtcgggctcg acgacgaatc cgctgcgcgt gctgctctgc
       61 cgcgatagcg cgcagccgct cggcctgctg accacctgcc acaccaactg atgttagctt
      121 ccgcccgcca tatgcagatg caagcttcgc tgctcgcgcg tgtcgccgcc ggcgatgcgt
      181 ctgcagtgcg cgaatgcatc gcgcagtatg gcgggctggt gtggtcgatc gcgcggcgct
      241 tcgagctcgc cgatgccgag gacgccgtgc aggaaatttt tctcgatcta tggaagtcgg
      301 ccgcgcgttt cgatccgaag atcgcctccg aggccgcgtt catcgcgatg atcgcgcgcc
      361 gccggctcat cgaccgccgc cgcacgcgcc gccggcgccc ctcgacggag cagcttcccg
      421 aggtgccgac acacgccgac gaagcctcgc tgcccgacac ctgcgccgaa gccaagcagg
      481 cggcccgggc actcgatcag cttcgtcccg agcagcggca agtgctcgtt ctctcgacgt
      541 gccacggcat gtcgcacggc gagatcgcgg cggaaaccgg catgccgctc gggacggtca
      601 aagcgcacgc gcgccgcgga cttttgtcga tccgcgcggc tcttctgggc gtacaaggag
      661 gagagtcgtg aagcccgcgc tgtcaccccg tgccgaggag ctgctcgccg agcgcgccgt
      721 gcgcccgctc gatgatcgcg agctgcacga gctcgatgcg ctcgacgcct cgaacgacga
      781 cagcttcgac ctcgccgccg cgagtgtcgc tgtcgcgctc acgccgctcg aaacgatgcc
      841 cgccgacctc gccgcaaaaa tcctcgccgc ctcgcccgcg ggcttcgacg caatgaagac
      901 ggtcccggga acatcgctga tgccaggcgc tattcgcacg ctccctggcg tgggcctcga
      961 cgcctatcgc ccgcagcccg acgcgcaggt tgtcgcgcgc gaaggcgagc cgcttgtcgc
     1021 atcgcgaggc gatgtccccg tcgcgcccca aggcgatgtg cgcgtcgcgc cgcaaggcga
     1081 tgcgctcggc gcgccaaagt ccgatatcga gcgcgctcgc gaggttcggg cgaaacgctc
     1141 gcgatttgca gtcgtcgcgc cgtggctcgc ggctgccgcg tgtctcttgg ctgccgtcgg
     1201 agcgctcgcg tgggcgcgcg atcaaggcag cggcacgaag acggtcgcga cgctctcacc
     1261 gagcgacgcg cgcgctgact tgctcgcgca cgccagcgac gtgaccacga tcgaatggac
     1321 gacgaccgcc gatcccacgg cgcaaggcgc aaagggcgat gtcgtgtgga gccagtccgc
     1381 gcagaagggc ttcatgcgct tcgcggggct cgcggtgaac gacgcgaagc agttccagta
     1441 tcagctctgg atcttcgaca agacgcgcga cgaggcattc cccgtcgacg gtggcgtctt
     1501 cgatgtgacc gcgagcggcg aggtcatcat tccgatttcg gcgaagctgc cggtcggcga
     1561 tgcgacgctg ttcgccgtga cgatcgagaa gccgggcggt gttgtcgtca gcaagcgcga
     1621 gagaatcgtc gtcacggccg cgcgcaagag ctgagaaatc ccggagggac tcgcacgaga
     1681 cctgtcctgg tctttgcaat acctctgggt atgactgcgt cagcgatttc cggagccgcc
     1741 gacaacgcga ttcacgaggc aaaaccgtgg ctagagaagc tggcgcgtgc cggcttcgtc
     1801 gcgaagggcc tgctgtacat gacgatcggt gcgctggcga ccgctgctgc attggggctc
     1861 ggtcgctcga cgacggggca aatgagcgcg gcgggtcacg cgggcccggc gagcacgatg
     1921 ggccagcgcg gtgccatggc agcgctgttg caagctcccg cgggtcgcgc attgctgatc
     1981 gccattgccg tcggcttgtt tggctatgcg gcgtggcgct tcatcgaagc gatccgcgat
     2041 ccgatgcatc acgcgaggtc ggagaacaag aagaaggcga agggcatcct gaagcggctg
     2101 cgctcggccg cgctcggcat catccaggtc gcgctcggca tcagcgcgct gaagatcgcg
     2161 ttcggtaaca tcgaagccgc gaacgacggc aaggcgagcg cccactggac cgggaaagcg
     2221 ctcgcgacgc caggtggcga gctcgtgctg tgggcgatcg ccggcggcct cgtcgcgtat
     2281 ggcgtgtatc agctctacaa ggcgtgggcg tcgaagctga gcaaggagct catgctgggg
     2341 cgtctgtcgt cgggcgcgcg tcgtctcgtc atcggcgcga gccgattcgg cattgcggcg
     2401 cgcggcatcg tgttcgcgac gaccggtatc ctcgtcgggc gggcgatcct gcagcgcgac
     2461 ccgcagcagg tgaagggcat caaggcttct ctgatcgagc tgctcgagct cggccgctgg
     2521 ccgttcgcga tcgtggcgat gggcctcatc gcgtacggca tctatcagct gatcaacgcc
     2581 cgctatcggc gcatcgagat cgcctgagcg cctgctagag tcgacggcgt gagacgcctc
     2641 gtcgacgctg ccctcgctct gcgcgatcgc gtgcgtgcgg cgcgagagtt
//
LOCUS       DATLBZ010000601         6313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7193858, whole genome shotgun sequence.
ACCESSION   DATLBZ010000601 DATLBZ010000000
VERSION     DATLBZ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7193858"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..82)
                     /locus_tag="VMZ53_29505"
     tRNA            complement(7..82)
                     /locus_tag="VMZ53_29505"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Ala,seq:ggc)
     gene            complement(123..875)
                     /locus_tag="VMZ53_29510"
     CDS             complement(123..875)
                     /locus_tag="VMZ53_29510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32688.1"
                     /translation="MARQRRGGGFRPPELPLRGTLGTLLRTAAAQVGPLRDALERTAR
                     EGLSDARSRLDDALAARASGSRSRAPHRPPSRATGSDERDVALADLGAIVLDLIRDGE
                     IDLAQLPEVRDLVAHLDELDASSSSDADPFHDDDSDEPYAPPPSRNRFDARGRDQSRE
                     DGSVSSKTWRPPPKKPPPRVWRPPVDNDEPAPSRHAARPADEITARSRPVPKPKDPLR
                     KGGISFDDDDADLADYMHPDDVPPKPPKDGDA"
     gene            903..2879
                     /locus_tag="VMZ53_29515"
     CDS             903..2879
                     /locus_tag="VMZ53_29515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32689.1"
                     /translation="MKRWLWLAPLLVVGTACPPPEPVAPPLPPVSGGKVRVRVFTEPA
                     PVKTVGTVGRFLFVATEDALQRWDEKGAVVTMTADQGLSGDHVVALATDPDRKWMWIL
                     TDGGLGHYDAGVEVYTEAAAPPTALGIEYSAIAKEGVASLAPATEGGVWLGTSKGLFF
                     VSEKGGWVATPVKDPVQALVRDRGGWLWIATKTGLVARKPSGDVVKIGAAQDCAVVAP
                     RLLVEAPGDRVMVIGADAQGRERLAIGKQMAWTSYRTLPEVKWTAATMKGGGIVVMGG
                     EKIYRIVQAPVGGGGGGVGVRPLAREGVRLVPLTGPTTSEWVIDPTTLVVPPGATVLG
                     AMEDQLLIGTRDLGTARYRDGDAHPRDWLRRKQMFDDATGLSVACARAQDCWLATGAR
                     QAWHWNGERFVAGGPDVVALAVARDPSGGIFALHRAPAEKELHLSRIDANGTWTQLQK
                     VTLTTPGDAPAVSFARFGGNGSLWIGLRYRDGQEMRAYGIAVVDVATGKVTYHRPDTR
                     EIPVPIGVVDADVRGDTAWFATSEGVARLADGQVKVWTEADGLRSELARAVTISPEGA
                     VIVATGAGAGVWDGKAWGFPPALRFEINDLVATRDGHVWMATERGIAAWDGKKVRRVD
                     MRRGLAENVVLDVATDQFDRIWARGPGSLTLISQ"
     gene            complement(2869..5220)
                     /locus_tag="VMZ53_29520"
     CDS             complement(2869..5220)
                     /locus_tag="VMZ53_29520"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUS32690.1"
                     /translation="MRGTLCLIAIAALAGCGDDPTKTITCGTGTSGALTASTAVEVTG
                     DAGKDLRGAAIAAGPGTTIPTGEVSIECAADINPPGTIALGPAVTFGADGTASDRPFL
                     LTLPYKKARLPKDAGRRHVRIVARRGGQPDSFFPLVSNRTLDDEDAYHSHITFRASEL
                     TTYQVVADEHAGTPETRQYSWRAITGISMGGYASMAIGLRHPDKFDMVADIGGDPGPS
                     MVYLLGWVRDYLFGGFCTVEDEAAGRGMVGQLCPHTPKSLNEIGANYEHMLTQVGEGV
                     GLTLRRNLYMKASRDLGRALGNPAHYNIDNPYTPPGVPYADTQIPAATRCANPRVLQD
                     FYDSEFNPTAAHPVITFCDGSESAALGNGVFDPNVPGTNPAELLLAVDLNANNKRDAG
                     EPVVTNAFEPFSDVGVDAKASKDEPGYDAATNPDPNRDDYHAIRNPLGTEGNGEFDTG
                     EPYVDSGLDGVMGTCQQGTTPPTGVSACYDFGEGDGKWNLSPNTQRWYDNDLMHRLSQ
                     LTPEQRHHMSLWFDAGIRDFLNNSVAADAAIAQAMAKYSLPFAVYDNFSILDGGTSDL
                     SYDFLDVPWAEIPKDGYLRYGNPDATEDQIMNGDGRHVGTANQIIYRVQTAFGWMNRR
                     WPDGDLDDTYDGGMLLKDLSFTSPTTGRVNPYGLSLPPGYNKPENANKRYPVIYFLHG
                     YGQDPRDLVDLSSVFALNMIASNPIESRFQKFIIVYVDGRCRPNMDGTPVDPAGDLCE
                     GGTFYMDAPLGGLARMETNLLDLMDYIDANYRTKAPSTASVTD"
     gene            5329..5691
                     /locus_tag="VMZ53_29525"
     CDS             5329..5691
                     /locus_tag="VMZ53_29525"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HUS32691.1"
                     /translation="MKVLADMHVAPPLRRWMTRLGLAVVVAIAIGYLPGELIRRDPRA
                     VKLERQLEEMHRESRELAAQNQALYREVEALRTDVGAVEARARADLGMVYPDEVVLRV
                     RRAPEVDPAKALAPAGAR"
     gene            5688..>6313
                     /locus_tag="VMZ53_29530"
     CDS             5688..>6313
                     /locus_tag="VMZ53_29530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32692.1"
                     /translation="MSGRESGPVPKRDRRAESKPVRKAENATTEVFARKKDDAAPKRT
                     PKIESAPIGRRNLDSAPVGKRGASLDDAPAGKRGAQLDDAPAGKRGAQLDDAPTGRRG
                     TESAPAKRMPQIDSAPVAMHAPLIESAPIGLRKASIPESGRIREGRGSDFDLPRPASG
                     TGQRAKIDLEHETGRRAKVDLEHETGRRAKVELDDGTTGRRAKVELDA"
BASE COUNT          977 a         1881 c         2362 g         1093 t
ORIGIN      
        1 tttaggtggt ggagataagg gggatcgaac ccctgacctc gtgaatgcca ttcacgcgct
       61 ctcccagctg agctatatcc ccgtaccgag gaccgggttt ctaccggccc gctctggagg
      121 tgtcaagcgt cgccgtcttt gggcggcttg ggcgggacgt catccggatg catgtagtcg
      181 gcgagatcgg catcgtcgtc gtcgaagctg attccgcctt tgcgcaatgg gtctttgggt
      241 ttcgggaccg ggcggctgcg ggcggtgatc tcgtcggccg ggcgggcggc gtggcgggac
      301 ggcgccggct cgtcattgtc tacgggtggg cgccagactc ggggcggcgg ctttttcggc
      361 ggcgggcgcc atgttttcga cgacacggag ccgtcttcgc gagattggtc acggccgcgg
      421 gcgtcgaagc gatttctcga cggcggcggt gcgtacggtt cgtcagaatc gtcgtcgtga
      481 aacgggtcgg catccgacga ggaggacgcg tcgagctcgt cgagatgggc gacgaggtcg
      541 cggacttcgg ggagctgggc gagatcgatt tcgccatcgc ggatcaggtc gaggacgatg
      601 gcgccgaggt cggcgagggc gacgtcgcgc tcgtcgctac cagtagctcg cgatgggggg
      661 cggtggggtg cgcgagagcg ggagccggag gcgcgggcgg cgagggcgtc gtcgaggcga
      721 gagcgtgcgt cgctgagacc ttcgcgggcg gtgcgctcga gggcgtcgcg gagcgggccg
      781 acttgggccg cggcggtgcg aaggagcgtg ccgagtgtgc cgcgcagggg cagctcggga
      841 gggcggaagc cgccgccgcg tcgttgccgt gccatgcgcc gagcgtatac cgtcgtcggg
      901 ctgtgaagcg atggctttgg ctcgcgccgc tcctcgtcgt cgggacggcg tgcccgccgc
      961 cggagccggt ggcgccgcca ctgccgccgg tgtcgggcgg gaaggtgcgc gttcgcgtct
     1021 ttacggagcc cgcgccggtg aagaccgttg ggacggtcgg gcggtttttg tttgttgcga
     1081 cggaagacgc gttgcagcgg tgggacgaga agggtgcggt cgtgacgatg accgccgacc
     1141 agggattgag cggcgatcat gtcgtcgctc tcgccacgga tccggatcgc aagtggatgt
     1201 ggattctgac cgatggtggc ttggggcact acgacgcggg cgtcgaggtc tataccgagg
     1261 ctgctgcgcc gccgacggcg ttgggcatcg agtattcggc gatcgcgaaa gagggtgttg
     1321 cgagcttggc gcctgcgacc gaaggcggcg tgtggctcgg aacgtcgaag ggcctgttct
     1381 tcgtaagcga gaagggcggc tgggtcgcga cgcccgtgaa ggaccccgtg caggcgttgg
     1441 tccgcgatcg cggcggatgg ctgtggatcg cgacgaagac gggattggtt gcacgcaagc
     1501 cgagcggcga tgtcgtgaag attggagccg cgcaggattg tgcggttgtc gcgccgcggc
     1561 tgttagtgga agcgcccggt gatcgcgtga tggtgatcgg cgccgatgcg cagggacgcg
     1621 agcgcttggc gatcggcaag cagatggcgt ggacgagcta tcgcacgctg cctgaagtga
     1681 agtggaccgc tgcgaccatg aagggcggcg gcatcgtcgt catgggcggc gagaagatct
     1741 atcgcatcgt gcaggctccc gttgggggcg ggggcggggg cgtgggcgtg cggccactcg
     1801 cgcgcgaagg cgtgaggttg gtgccgctca ccggaccgac gacgagcgag tgggtcatcg
     1861 atccgacgac gctcgtcgtg ccgcccggcg cgacggtgtt gggcgcgatg gaagatcagc
     1921 tgctgatcgg cacgcgcgat ttggggacgg cgcgctatcg cgatggcgat gcacatccgc
     1981 gcgattggtt gcgtcgcaag cagatgttcg acgacgcgac tggattgtcg gtcgcgtgcg
     2041 cgcgtgcgca ggattgttgg ctcgcgacgg gtgctcggca agcgtggcac tggaatggcg
     2101 agcggttcgt ggctggcggg cccgacgttg tcgcgttggc ggttgcgcgc gatccgtctg
     2161 gcggaatttt tgcgctgcat cgcgcgcctg cggagaagga gctgcatctc tctcgcatcg
     2221 atgcgaatgg cacgtggacg cagctgcaga aggtcacgct gacgactccg ggcgatgcgc
     2281 cggcggttag cttcgcgcgc tttggtggca atggatcgct gtggatcggg ctgcggtatc
     2341 gcgatggcca ggagatgcgc gcgtatggca tcgccgtcgt cgatgtcgcg acggggaagg
     2401 tcacgtatca ccggcctgat acgcgcgaga ttccggtgcc gatcggcgtc gtcgatgcag
     2461 acgttcgcgg cgataccgcg tggttcgcga cgagcgaagg tgttgcgcgg ctcgccgatg
     2521 gacaggtgaa ggtctggacc gaagccgatg gcttgcgcag cgagctcgca cgcgcggtga
     2581 cgatttcacc ggagggcgcg gtcatcgtcg cgaccggagc gggcgcgggc gtgtgggacg
     2641 gtaaagcgtg gggctttccg cctgcgctgc gattcgagat caatgatctc gtcgcgacgc
     2701 gcgatgggca tgtgtggatg gcgaccgagc gcggcattgc ggcgtgggac ggcaagaaag
     2761 tccgtcgcgt cgacatgcgc cgcgggcttg cggagaacgt cgtgctcgat gtcgcgaccg
     2821 atcagttcga tcgcatttgg gcgcgcggtc cgggcagcct gacgctgatc agtcagtaac
     2881 cgacgcggtg ctcggcgcct tcgtgcggta gttcgcgtcg atgtagtcca tcaggtcgag
     2941 caggttcgtc tccatgcgag cgagaccgcc gagcggggca tccatgtaga acgtgccgcc
     3001 ttcacagaga tcgcccgctg gatcgaccgg cgtgccgtcc atgttcgggc ggcagcgacc
     3061 atcgacgtac acgatgatga acttctggaa ccgcgactcg atcggattgc tcgcgatcat
     3121 gttgagcgcg aacacgctcg agagatcgac gaggtcacgc gggtcctggc cgtagccgtg
     3181 caagaagtag atgaccgggt agcgcttgtt cgcgttctcc ggcttgttgt agccgggcgg
     3241 cagcgagagg ccgtacggat tcacgcgtcc cgtcgtcggc gacgtgaagg agaggtcctt
     3301 gagcagcatg ccgccgtcgt acgtgtcgtc gagatcgccg tcgggccagc gccggttcat
     3361 ccagccgaac gcggtctgca cgcgatagat gatctggttc gcggtgccga cgtggcgacc
     3421 gtcgccgttc atgatctgat cttccgtcgc gtcgggattg ccgtagcgca ggtagccgtc
     3481 cttcgggatc tccgcccacg gcacgtcgag gaagtcgtag gagagatcgc tcgtgccgcc
     3541 gtcgaggatc gagaagttgt catagacggc aaacggcaag ctgtacttcg ccatcgcctg
     3601 cgcgatcgcg gcgtctgcgg cgacgctgtt gttgagaaag tcgcgaatgc ccgcgtcgaa
     3661 ccacaggctc atgtgatgac gctgttcggg cgtcagctgc gagaggcggt gcatgagatc
     3721 gttgtcgtac caacgctgcg tgttcggcga gaggttccac ttgccgtcgc cttcgccgaa
     3781 gtcgtagcaa gcagagacgc ccgttggcgg agtcgtgcct tgctggcagg tgcccatcac
     3841 gccgtcgagg ccgctgtcga cgtacggctc gccggtgtcg aactcgccgt tgccttcggt
     3901 gccgagtgga ttgcgaatcg cgtggtagtc gtcgcgattc ggatcgggat tcgtcgccgc
     3961 gtcgtagccg ggctcgtcct tcgaggcctt tgcgtcgacg ccgacgtcgg agaacggctc
     4021 gaacgcgttc gtcacgacgg gctcgcccgc atcgcgcttg ttgttggcgt tgagatcgac
     4081 tgcgagcagg agctccgcgg ggttggtccc gggcacgttc ggatcgaaca cgccgttgcc
     4141 gagcgcagcg ctctcgctgc cgtcgcagaa cgtgatcacg ggatgtgcgg ccgtcgggtt
     4201 gaactcgctg tcgtagaagt cttggaggac acgcgggttc gcgcagcgcg tggcggcggg
     4261 gatttgggtg tcggcgtacg ggacgcccgg tggcgtgtac ggattgtcga tgttgtagtg
     4321 agcggggttg ccgagcgcac ggccgaggtc gcgcgacgcc ttcatataga ggttgcgccg
     4381 aagcgtcagg cccacgcctt cgccgacctg cgtgagcatg tgctcgtagt tggcgccgat
     4441 ctcgttgagg ctcttcggtg tgtgcgggca gagctgaccg accatgccgc gtccggctgc
     4501 ttcgtcctcg acggtgcaga acccgccgaa cagatagtcg cgaacccagc cgaggaggta
     4561 gaccatcgac ggacccgggt cgccgccgat gtctgcgacc atgtcgaact tgtcgggatg
     4621 gcgcaggccg atcgccatcg acgcgtagcc gcccatcgag atgcccgtga tcgcgcgcca
     4681 cgaatactgc cgcgtctccg gcgtacccgc gtgctcgtcc gcgacgactt gataggtcgt
     4741 gagctcgctc gctctgaacg tgatgtgcga gtggtacgcg tcttcgtcgt cgagcgttcg
     4801 gttcgagacg agcgggaaga aggagtccgg ctgaccaccg cgccgcgcga cgatccgcac
     4861 gtggcgacgt cctgcgtcct tcggcagtcg cgccttctta taaggaagcg tcagcaggaa
     4921 cggtcgatcc gatgcggtgc cgtcagcgcc gaatgtgacg gcggggccga gcgcgatcgt
     4981 tcccggcggg ttgatgtccg ccgcgcactc gatcgagacc tcgccggtcg ggatcgtcgt
     5041 gccgggaccc gccgcgatcg cagcgccgcg cagatctttc cccgcgtcgc cggtcacttc
     5101 gactgcggtg ctcgcggtta gcgcgccgct cgtgccggtg ccgcaagtga tggtcttagt
     5161 gggatcgtca ccgcagccag cgagcgcagc gatagcgatc agacaaagcg tccccctcat
     5221 gtgcgcgcag cgtacacata ggccgcggaa actccatcgg aaaatgatcg gctgtgacgg
     5281 ccgccaaaag ttggccccgc aaaggcgcga tccgtaacgt cggacagagt gaaggtcctc
     5341 gccgacatgc acgtggcgcc tccgctgcgc cgctggatga cacggctcgg cctcgcggtc
     5401 gtcgtagcca tcgccatcgg ctatctgccg ggcgagctga ttcgccgcga tccacgcgca
     5461 gtcaagctcg agcgtcagct cgaagagatg cacagggaat cgcgcgagct cgctgcgcag
     5521 aatcaggcgc tctatcgcga ggtcgaggcg ctgcgcaccg acgtcggcgc ggtcgaagcg
     5581 cgtgcgcgcg cggatctcgg catggtgtat cccgacgaag ttgtcctccg cgtgcgtcgc
     5641 gcgcccgagg tcgatcccgc aaaggcgctc gcgcctgcag gtgcacgatg agcggccgcg
     5701 agtccggccc cgtgccgaag cgcgatcgac gcgccgagag caagccggtg cgcaaggccg
     5761 agaatgcgac gaccgaggtg ttcgcgcgca agaaggacga cgcggcgccc aagcgcacgc
     5821 cgaagatcga gagcgcgccg atcggcaggc gcaacctcga cagcgcgccg gtcggcaaac
     5881 gcggcgcttc gctcgacgac gcaccggccg gcaaacgcgg cgctcagctc gacgacgcac
     5941 cggcgggcaa acgcggcgct cagctcgacg acgctccgac tggccgccgc ggcaccgaga
     6001 gcgcgcccgc gaagcggatg ccccagatcg atagcgcgcc ggtcgcgatg cacgcaccgc
     6061 tcatcgagag cgcgccgatc ggcctgcgca aggcatcgat tcccgagagc ggccgcatcc
     6121 gggaaggacg tgggagcgat ttcgatcttc cccgcccggc gtccggcacg ggacaacgcg
     6181 caaagatcga cctcgagcac gagaccggtc gtcgcgcgaa ggtcgacctc gagcacgaga
     6241 ccggtcgtcg cgcaaaggtc gagctcgacg atggcacgac tggccggcgc gcgaaggtcg
     6301 agctcgacgc gca
//
LOCUS       DATLBZ010000602         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3487176, whole genome shotgun sequence.
ACCESSION   DATLBZ010000602 DATLBZ010000000
VERSION     DATLBZ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487176"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VMZ53_29535"
     CDS             complement(<1..443)
                     /locus_tag="VMZ53_29535"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32693.1"
                     /translation="MSSSDDPLLALMREVAAAPAIELGALAEPLRAGLVIGRFELVRE
                     LGRGGFGIVWEAHDRELDRHVALKTLASARLSAEGIESFRREARVVAKLAHPNLVTLH
                     DVLVHDGAPVLVLELLHGETLENRLAKRPLALPTALEIALALARGL"
     gene            complement(440..1021)
                     /locus_tag="VMZ53_29540"
     CDS             complement(440..1021)
                     /locus_tag="VMZ53_29540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS32694.1"
                     /translation="MTEATVDSALEQLLAAKQFEQATVVAIQTLGPQVLGYLTAILGD
                     HERARDVFSVFSEDLWSGLPGYRGDGSFRAWVYRIARNAALRTVRDPYWRRGRRLATE
                     EHSALAAAVRSTTKAHARPSADAKLARLRSALTPDEHALLILRVDRDLSWSEIASALT
                     TGKRKPDEAALRKRFERLKAKLRRLAEAEGMLR"
     gene            complement(1026..1895)
                     /locus_tag="VMZ53_29545"
     CDS             complement(1026..1895)
                     /locus_tag="VMZ53_29545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS32695.1"
                     /translation="MSEEQKANAKRALDDGNALLLDKKYVEALDKYTEAVAQWDHPAI
                     RFNMVRCQIQLGKNLEAYENLEKALKYGADPLEETVYNEAIAYQKLLETQIGNIQVTC
                     KQDGVKLTIDGQPLASCPAQESRRLLAGPHQIVGQKPGLLPKTMDVVVVGGKPLGVDV
                     KLDPLAKGAKIVHRWPGYVPWTVFGGGLALGAIGGLTLWAASTQLRSYDVFVDANCTS
                     QCTDAQLAQAKHDYYDPAQTKNNVGGTFVAVGAAAVATGAVMVYLNRGRTVYPDVAPL
                     PGGGGAVSLSGTF"
     gene            complement(2008..>2401)
                     /locus_tag="VMZ53_29550"
     CDS             complement(2008..>2401)
                     /locus_tag="VMZ53_29550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32696.1"
                     /translation="PATQGAFGLDGIVTADDAIYVNKLDSSQLFRIGEDQAVTEITVT
                     PRLVAPDGMRAIDADTLLVIEGGANRLSKVEIHGTTATSTVLSSDLDQPTAVAISRGS
                     AWVSEGQLGRLFTGQSATLPFSVRRVAL"
BASE COUNT          382 a          799 c          799 g          421 t
ORIGIN      
        1 agtccgcgcg ccagtgccag tgcaatctcg agcgccgtcg ggagcgcgag cggacgcttc
       61 gcaaggcgat tctcgagcgt ctcgccgtgc aagagctcga ggacgagcac cggcgcgccg
      121 tcatgcacga gcacgtcgtg cagcgtgacg aggttcggat gtgcgagctt cgcgacgacg
      181 cgcgcctcgc gtctgaacga ctcgattccc tctgcggaca gacgagcgct ggcgagggtc
      241 ttgagcgcca cgtgccggtc gagctcgcgg tcatgcgctt cccagacgat cccgaacccg
      301 ccgcgtccga gctcacgcac gagctcgaac cgtccgatca cgagcccagc tcggagtggt
      361 tcggcaaggg caccgagctc gatggcgggc gccgccgcca cctcgcgcat cagagcgagc
      421 aacggatcgt cgctcgagct caccgcagca ttccttcggc ttccgctagc cggcggagct
      481 tcgccttcag acgctcgaac cgcttgcgta acgcagcttc gtccggcttg cgctttccgg
      541 tggtgagcgc gctcgcgatc tccgaccatg acaggtcgcg gtcgacgcgg aggatcaaca
      601 atgcgtgctc gtcgggcgtc agtgcgctgc gcagtcgcgc gagctttgca tccgccgacg
      661 gtcgcgcgtg cgccttcgtc gtcgaccgca ccgcggcagc gagcgcggag tgctcttctg
      721 tcgcaagtcg ccgcccacgc cgccagtacg gatcgcggac agttcgcagc gctgcattgc
      781 gcgcgatcct gtacacccac gcccggaacg atccgtcacc gcgatatcca ggcagccccg
      841 accacagatc ttccgagaac accgagaaca cgtcgcgtgc gcgttcgtgg tcgccgagga
      901 tcgcggtgag ataacccagc acctgagggc cgagcgtctg gatcgcgacg actgtggcct
      961 gctcgaattg tttcgccgcg agcagttgct cgagcgcgct atcgacggtg gcctcggtca
     1021 cgccctcaga acgtgccaga aagcgatacc gctccgccac cgccggggag cggcgcgacg
     1081 tcgggataca cggtgcggcc gcggttgaga tagaccatca ccgcgccggt cgcgacggct
     1141 gccgcgccga ccgccacgaa cgtgccgccg acattgttct tggtttgcgc ggggtcgtag
     1201 tagtcgtgct tggcttgtgc gagctgcgca tcggtgcact ggctggtgca gttcgcgtcg
     1261 acaaacacat cgtacgagcg cagctgcgtg ctggcggccc agagcgtcag gccaccgatg
     1321 gcgccgagcg cgaggcctcc gccgaacacg gtccagggca catagcccgg ccagcgatgc
     1381 acgatcttcg cgcccttcgc gagcgggtcg agcttgacgt cgacgccgag cggtttgccg
     1441 ccgacgacca cgacgtccat cgtctttggt aggaggcccg gcttctggcc gacgatctga
     1501 tgcggtccgg cgagcaggcg gcgtgactcc tgcgcgggac acgacgcgag cggctgacca
     1561 tcgatcgtca gcttcacgcc gtcttgcttg cacgtgacct ggatgttgcc gatctgcgtc
     1621 tcgagcagct tctggtatgc gatcgcctcg ttgtagacgg tttcctcgag aggatcggcc
     1681 ccgtacttca gcgccttctc gaggttctcg taggcttcga ggttcttccc gagctggatc
     1741 tggcagcgaa ccatgttgaa gcggatcgcc ggatgatccc actgggcgac cgcctcggtg
     1801 tacttgtcga gtgcctcgac gtacttcttg tcgaggagca gcgcgttgcc gtcgtcgagc
     1861 gcgcgctttg cgttcgcctt ctgctcttca ctcacgcctt gagaccatgg cgcgcttggc
     1921 ggcgcgtccg cgtgtacgac cgcgggaagg agcgagaacg cgattgcgaa ggcaagcgtt
     1981 cgccgggaag cgaccatcga aaccagtcta cagcgcaacg cgacggaccg agaacggaag
     2041 agtcgcgctt tgacctgtaa acaaacgccc gagttgtccc tcactcaccc acgccgagcc
     2101 tcgtgaaatc gcgaccgcgg tgggctggtc gaggtcgctc gagagcacgg tcgatgtcgc
     2161 cgtggtgccg tggatctcga ccttcgacag gcggttcgcg ccgccttcga tcacgaggag
     2221 cgtgtcggcg tcgatggcac gcatgccgtc cggggcgaca agccgcggcg tgaccgtgat
     2281 ctccgtgacc gcctggtcct caccgatgcg gaacagctgg ctcgagtcga gcttgttgac
     2341 gtagatcgcg tcgtccgcgg tcacgatgcc gtcgaggccg aatgcgccct gggtcgcggg
     2401 g
//
LOCUS       DATLBZ010000603        17668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10921623, whole genome shotgun sequence.
ACCESSION   DATLBZ010000603 DATLBZ010000000
VERSION     DATLBZ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17668
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10921623"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VMZ53_29555"
     CDS             <1..878
                     /locus_tag="VMZ53_29555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS32697.1"
                     /translation="MKLDPGIGTSLNIGKCYEEWGRIGKALVAYQTALKMAKDDKDKR
                     APQIQELVDNLDSQVPRLTIKLPKDASQDGVKVTLDGEAITTFGTSLIIDPGPHTIEW
                     TVDDGAKKSRVVPVERGGDSEVTLDIPKERVAKKPPGDGKGKTGGGTKSAGGGDGTGI
                     KPAEATAPAPGRNMRIGGFVLGGAGVIAMGVASIMTLSAKGQYDDALEMHCMGMKNMC
                     DPDGLTATADARSTANTATILFLGGVALVGGGVALYLLAPKGAAQTESRDETARYIVP
                     SVTKDGAGVVFGGRF"
     gene            complement(910..1173)
                     /locus_tag="VMZ53_29560"
     CDS             complement(910..1173)
                     /locus_tag="VMZ53_29560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32698.1"
                     /translation="MLKKSGFLIVTLVLMAFAVEARAEHVDWSSYIDPNPSKPVTSKP
                     VTVVSDDAPKAKSSGKASKRVAKKATSKRKVKARAKSKKSRRK"
     gene            complement(1397..2368)
                     /locus_tag="VMZ53_29565"
     CDS             complement(1397..2368)
                     /locus_tag="VMZ53_29565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32699.1"
                     /translation="MSAATATAPGCSRCESPLEEGDLRCSVCALPVPVAPMPIEKVRT
                     KILRCTECGAAVAFDANKQAPSCAFCCATMKVEQPVDPIEVAQLVVPFSVSLDLAQAA
                     LRTWLGGRGFFAPKTLKDEAVFETLQPLCWAAWIVNATAEVSWTADSDAGSQRSDWAP
                     HAGVIHERFGNIVIPASRGLTPVECSLLGPYYDLSTVEGVGTVEPSMIESFDAQRSAA
                     RQQVARSIEAVAKNRVEKIVPGRRTRNIHVSCLVESQTTDRVALPAWVLAYRYRGSPY
                     RAIVHGQRPEAVFGTSPTDWGKVAVVVLAGLAAAVAIIALILYLTHR"
     gene            complement(2365..3270)
                     /locus_tag="VMZ53_29570"
     CDS             complement(2365..3270)
                     /locus_tag="VMZ53_29570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32700.1"
                     /translation="MTTFACQNCGAGLAFEGVRTETCPFCASPNFVERPPAQGQPDPQ
                     FVVTFVGDGEVARRALDRWLGSRTIFADSRLKQAKVEDIKGIYAPSYLYSAVSRSDFT
                     AQIGENYTETETYTETDSEGRSHTRTRTVTRTEYRPLAGQHVSYVTDVIVSASAGLSN
                     AELESVEPYDLKQMRRYSPALISGWIAEEFARPPSECETSSRREAVDQIGGKLRRYMP
                     GDSYSDLTWRTRVSWESLDPIFVPVWVFAVRYREDKPPLKVVINGQTGKIAGKVPLSW
                     IKIVATIVFVLAAIAAAILLTRPHV"
     gene            complement(3274..4569)
                     /locus_tag="VMZ53_29575"
     CDS             complement(3274..4569)
                     /locus_tag="VMZ53_29575"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HUS32701.1"
                     /translation="MVLSRIVLATLLLTGCKLSLFADHGGGDDIAGDANDNGDVMVQS
                     SCGAMCLGDAGADLGKTGTKWRYLDDHRDRTWTPMTAAGTSYVGANTMNKFSPCAGSS
                     AAACTMLPGALLVSTAGKSDAADPALEFSSSSNQVIKISLRVYIENGGDHMVRLYRNA
                     REDSLYTFTATQGNMLETSITLDALAGERFLLALAPTNAGAQDIGVQLFINGTTTVFP
                     QECQMALQFNEAATGNMIPNPCGGAALSRHYNMDNSFNDVDPTQTAGPFPEMGMAASL
                     TFENYYRGGDIMDRMGDTTTQFWVRQDMEGTENAVLVSDDDLDKPGGLAVYKLMGGGN
                     IGAQTCVDANTLEHHYFDAPYPAPANGWHFVRVIQAGTAVRICVDGVRLGSYVLAAGA
                     MQSDYPLFVGKNGPWLPAGAFFQGAIDDVRVLKGALPCN"
     gene            complement(4560..5600)
                     /locus_tag="VMZ53_29580"
     CDS             complement(4560..5600)
                     /locus_tag="VMZ53_29580"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS32702.1"
                     /translation="MKKLAFIVVLASSAVALAGPNKDKPAAGEQKEADKHFKAGVALF
                     KEAKYGEALAEFERAYEIAPHPLVLYNIATCHRELSHYGEAVKFYRRFIKEAPAAKVS
                     SAKIKTAQSELDGVLARIARVSVSVSSGAGTELLVDGISLGTAPFEEPIILPPGEHKL
                     TARAVGHKDAERTVRVASGDEVEVTLTLGETPAETKATVIEEPAVRDKIEEPVAPTRT
                     TKRFSIGAAFGTNLLEAGDTGAPTLGLGVAINDRIQVGVDAVVVAYAVMPSVRVRLAG
                     NAVSVHLAAAMPISITDDGMSETFVAGAVGLGLRYRPMPSFSIRLESFASFAGKTHGT
                     TLPAFVGGELWF"
     gene            complement(5597..7183)
                     /locus_tag="VMZ53_29585"
     CDS             complement(5597..7183)
                     /locus_tag="VMZ53_29585"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32703.1"
                     /translation="MEAAAGQRQLPPGTMLDAKYRIESLLAVGGMGAVYVGTHVALRK
                     KVAIKVLNPQLTSPAMLQRFQREAITASQIGHEGIAQVTDLGTSTDGQAFLVMELLEG
                     ESLAGRLKATGPLPVELACELGCAILSPLAAAHAAGIVHRDLKPDNVFLVRQSRGEMV
                     KLLDFGISRAQGLEAELRLTNTGVVMGTPYYMSPEQARGDSEVSHASDIYSIGVILYE
                     MLVGEVPIKGDNYNQLMFRVMSGEYARLRQRRPEIPVELEAIIERAMATDASLRWTSA
                     SELEHALLAFCRPGYREHMIERLSNAGIPWRATPMPYSSSQPGYGSQPGYGSQPGYGS
                     HPGTGPGVQAPINQGTDPTLLAPSYIRQPAKRSKLPLIIIAAVLIGGGAAAFIAMSGG
                     DGSTKTAANEPAPTPPVDTSPSAAPTPPAPTPAPPVASPPPPAEPATVTLKLSVTPAT
                     ATIKLDGKPVTGDITQPKDDSEHELSVTAPGYVAHSEKVRFDESQRISVALAKQVKAP
                     VGGHKTNGKRDKIEVDSPYK"
     gene            7265..8353
                     /locus_tag="VMZ53_29590"
     CDS             7265..8353
                     /locus_tag="VMZ53_29590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32704.1"
                     /translation="MCPAGAPAEASPLDIVGLGSRRAGEANTGLASVDDASALYYNPA
                     GLVAHAACRLEQEPEGDYDESPDPICVERDRGDFIIGVTGAYSHLAINKVRAPFNDAG
                     GAQLAARARITERFALAFAFHGPSQGGVHADVRDPEYPLYPYYRDRLSRPVLLVGAAT
                     SLSDAARAGAALDLGAGTSHPKAIAGVTLRVSPSLQAGVVWRQRFAIIDDTTGARVHY
                     TPHTVLAGLTFANDAIRASVDAGFAHWSAYKGPFGSDSTVRVPYKSTGQLRLGLESNK
                     DVGVSFRVGYAFESAAVPPTQPTSNLLDGSKQTVAGGMSYGQGNIRVDAYVGLQIVGS
                     RSMTDPDDTTLKSGGEIFTAGLSLAVGY"
     gene            8608..9645
                     /locus_tag="VMZ53_29595"
     CDS             8608..9645
                     /locus_tag="VMZ53_29595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HUS32705.1"
                     /translation="MVATVIPRVARPPAADELTLADTDSVRLMLRGDSIIDWHRLNFA
                     DHAEVDRFLRLNEFDPESDPEMDRFEDIRADAVDYLARAFAMAIPDEVAADLPARDLF
                     LVASSSGPHQKWACVVLKVMHIIHHINGRQALLKVSVSDEIIFREIEMKVLQVVEQLR
                     AAGAPLTEFEWSRKPRDSQITKLLAKRSTLAASIYDKLRFRLIVPHYADIVPMLATLT
                     RQLIPFNYVVPGESVNQLVDLSHFSPDSSGIMRAPDAMPFNEFSGPEYRIINFVADLP
                     LRLERLIPKNEQHPELSHVVFVLTEFQVCDKATAQRNESGASSHEAYKQRQHERVRMR
                     LFRDKDDPIPE"
     gene            complement(9646..10227)
                     /locus_tag="VMZ53_29600"
     CDS             complement(9646..10227)
                     /locus_tag="VMZ53_29600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32706.1"
                     /translation="MTPYESVDALIARIDEPNRSACARIVADNRALFATVQGSTNNHQ
                     AWRGGYLDHITEVMNIALVLYDRLSALRPLPFSRSDILLVVFLHDIEKPWKYELGADG
                     QLHHKANMQGKEAHHAFRLEMLERYGVALTAEHENGLRYAEGELSDYTNKERRMGPLA
                     AMAHMCDVASARLWFDYPAAVDDPWPGATRVAT"
     gene            complement(10263..11834)
                     /locus_tag="VMZ53_29605"
     CDS             complement(10263..11834)
                     /locus_tag="VMZ53_29605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUS32707.1"
                     /translation="MLGRWLAVLSLVTAASSARADDAAPAAPYVPPDFLSATPPLPDT
                     VDAANTLRLDLPQALQMAVRQNLGVTLERRETHIAELGVDVARGLFEPEVDATYAHGS
                     SRTPPSSAQEGMAGEILRFTDDRWDLSINQRLATGMQLSATWTSSRSDSSLGTAVEPI
                     NYRSALTVGIRQPLLRGFSFDLDLPQLSVLQARISSERERRQLSVVVAEVVERTEAAY
                     WEVLRALYNYDLQRRSQKRAEEQLDLTKRQIAAGAIPPADLISAESTLAQRQLQVVQA
                     EQLIEASWDQLRSIINMPRDEWTKPILPVDVPHFEPHALPAAQALETALKHRPEIEQI
                     DLELRASELSVRKADNDKLPQIDLGLSGSVVGQDGQYSGALSQLGGFEARGWNVFVNF
                     TWTPLRRATSAQAEIERTRHEIATARREQLVQQIWLAVREAVRNQDNAARQVAAAAHF
                     RQIAEKTLEVEQRKFLNGSSSQVVVAQRQEELAAAQIAEVGAVLDHTKAVAALGRATG
                     ELLGQKHIELSVGAK"
     gene            11857..13653
                     /locus_tag="VMZ53_29610"
     CDS             11857..13653
                     /locus_tag="VMZ53_29610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32708.1"
                     /translation="MGESNDTRAPANADTVDANTALTEAAEREADNLPFELPPPGYQL
                     GTMIGKGGMGEVMAAHDQRIGRDVAFKRMRNADASGDALARFLREARIQARLDHPAVV
                     PVYELGKDAEGRPFFTMKRLTGKTLTAHLADKDAPRQPLLRAFAEVCLAVELAHSKGV
                     IHRDLKPSNIMLGDFGEVYVLDWGIARVMGGRRRAPTNGMQADDIDSVPGEGTMTGDM
                     LGTPGYMSPEQMRGQLDVGPKADVYSLGSILFEILTGEPLHPRGHASVASTLTMEEAS
                     PAQRKPERNIAPELDAACASTLVADPQKRPTARELAEKITRYLDGDRDVERRRAIAAE
                     NLATARTLLAEGDAGKRAEAMRAAGRALALDPESKDAAQLVSSMIVEAPAQNPPELVA
                     EIEANEIRGAKTRALKGMIGLLTLSSLSVLVLFLEVRSWAWVIAIWVTLASLAGLMWT
                     TYRTGRVYGVAILLYGVALAIEFSRIASPLILVPALMCGAMLPFSNIPRINDRPIVLY
                     VWCLAVAMVPSLLELVGVLTPTLRIGEGNFVELYSEVFVHSPRAGIVSLVIANAVLLL
                     TVARYAIAIGRDRRDVQGKLTNQAWHLRKLLP"
     gene            13672..15537
                     /locus_tag="VMZ53_29615"
     CDS             13672..15537
                     /locus_tag="VMZ53_29615"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32709.1"
                     /translation="MVDDDHRGTRLDPGAPTVDGSMAGAGNAPTELPSATDETIVPSW
                     GASEIAALPEPGYQIGELIGRGGMGEVVAAQDQRIGREVAVKRIRSLEPSADAVMRFL
                     REARIQARLDHPAIVPVYELGTDAEGRPYFTMKRLAGVTLAKKLQQQGGPVQPLLRAF
                     VDVCLAIQLAHSRGVVHRDLKPSNIMLGDYGEVYVIDWGVARVLTDTKRTTGPALAVD
                     PDDDTTAGSILGTPGYMAPEQVRGAEAGTKADVYALGAILFEILAGEPLHPRGEAALA
                     STLTSPQEAPTRRVAERRQIPPELDGVCFDALAEEPAARPSARELADRMQAYLDGDRD
                     VERRRTMAAQQLASAREALDTNDRATAIRRAGRALALDPESADAATLVTSLLLEPPDE
                     MPKDLVEQLAADDKVFNSSRSRQGAWTYLSLFSFWLVIPFMGVKSWTALIAFYGLLGL
                     GAALAWRNSNRGYSSIPVTFVMTMLVALMFTRISGPFILTPVVICAALMQLAAIRQVA
                     ERLWLVVSWVVLAVMLPFALEWTHLIDPTYEVNKGLIISTSKIFDMRTGIDEATLVIS
                     NLLFILAAGMVAVFISRRRMKAQRQLQIQAWHLRQLLPTAKRWQTQPRGRRTLLG"
     gene            15534..17351
                     /locus_tag="VMZ53_29620"
     CDS             15534..17351
                     /locus_tag="VMZ53_29620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32710.1"
                     /translation="MSRPPGGDDPTATPDLDTAPASHDSMPVLPELPGYVPGETIGRG
                     GMGEVIAARDVTLDREVALKRMKMQSPTSDAIARFMREAKIQARLDHPSIVPVHEMGT
                     DVNGNPYFTMKRLSGRTLAQVLESRTLSLQALLRAFVDVCFAVERAHAADVVHRDLKP
                     SNVMLGDFNDVYVIDWGVARVMRTKRTSSMAAAVDIDSLPVDETGVGVMLGTPGFMAP
                     EQMKGDDVSPAADVYSLGALLFEMLAGEPLHPPGKQAISHTLASPTESPAKRKPERAV
                     PPELDELCVAALDEDPAKRPTARELGDRVQRYLDGDRDLERRRTLAAEQLAKARKLVT
                     EPARRTEGGQMAARALALDPESKEAAELVSRLILEPPKELPPALVESLEDSERKLNAQ
                     RGRSAMIAFLTLWLVLPVFIIFQHIKDWTQLALLYAVVTLMAILSWENGRTGRTPTWM
                     TAFGNFAVAVMFSRLTGSFVLTVGLVAGQTLALSSRPWFVHNRKWMIAWIVVSLLTPF
                     TLEWFGLIESTWHMAPGGLYVTGTILDTTHTRDVVVLAFSQCALAVVVGLFGMSITRA
                     REEAQRRAHIQAWHLQQLLPGANKDITGSGSADKLASTR"
     gene            17442..>17668
                     /locus_tag="VMZ53_29625"
     CDS             17442..>17668
                     /locus_tag="VMZ53_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS32711.1"
                     /translation="MAGPLIELVDVARYYQMGDERIAALDGVSFTVHEGEMVAIVGTS
                     GSGKSTLLNILGMLDTPSGGRYRLGGRDVQDL"
BASE COUNT         2917 a         5863 c         5831 g         3057 t
ORIGIN      
        1 cgatgaagct cgaccccggc atcggcacgt cgctcaacat cggcaagtgc tacgaggagt
       61 ggggccgcat cggcaaggcg ctcgtcgcgt atcagaccgc gctgaagatg gcgaaggacg
      121 acaaagacaa gcgcgcgcca cagatccagg agctcgtcga caacctcgac tcgcaagtcc
      181 cgcggctcac gatcaagctg cccaaagacg cctcgcaaga cggcgtcaag gtcacgctcg
      241 acggcgaagc catcacgacg ttcggcacgt cgctgatcat cgatcccggc ccgcacacga
      301 tcgagtggac cgtcgacgac ggcgcgaaaa agtcgagggt cgtgccagtc gaacgcggcg
      361 gcgattccga ggtcacgctc gacatcccca aggagcgcgt cgcgaagaag ccgccgggcg
      421 atggcaaagg caagacgggc ggtggcacga agtcggcagg tggcggcgac ggcactggca
      481 tcaagccggc cgaagctacc gctccggcac ccggtcgcaa catgcggatc ggcggcttcg
      541 tgctcggcgg tgcgggcgtg atcgcgatgg gcgtcgcgtc gatcatgacg ctgtcggcga
      601 agggtcagta cgacgacgcg ctcgagatgc actgcatggg catgaagaac atgtgcgacc
      661 ccgacggcct caccgccacg gccgacgcac gcagcacggc aaacaccgcc acgatcctct
      721 tcctcggcgg cgtcgcgctc gttggcggcg gcgtggcgct atacctgctc gcgccgaagg
      781 gcgctgcgca gacggagtcg agagacgaga ccgcccgcta catcgtgccc tcggtgacga
      841 aggacggagc gggcgtggtg ttcggcggtc gcttctagcg ccgcgggcgg gcggcgcggt
      901 tgttgcgcgc tacttacggc ggctcttctt cgacttcgcg cgggctttca ccttgcgctt
      961 gctggtcgcc ttcttcgcga cgcgcttgct cgccttgccg ctcgacttcg ctttcggggc
     1021 gtcatcgctg acgaccgtca cgggcttcga ggtgaccggc ttcgacgggt tcgggtcgat
     1081 atacgagctc cagtcgacat gctcggcgcg cgcctccacg gcgaacgcca tcaggacgag
     1141 ggtgacgatg agaaagcctg atttcttgag catggggtcc tcccggtttg actccgcctg
     1201 tactgcaagt gcggtgtcgg gcccctgtgg ttgcagaaat cgttcgcagt gtggggctcc
     1261 aaccccgccg gtcgagcgtg caggcatttc gccgcgttag gccctccgga gatcgtttgg
     1321 acagcaacga tctggggact ggacccgcag tccagggagt cggctctccg tcgcaaaatg
     1381 ccccggctgg gtcgggctac cggtgcgtca ggtacaggat cagcgcgatg atcgcgaccg
     1441 ccgcggcgag gcccgcgagc acgacgacgg cgaccttgcc ccagtccgtc ggtgacgttc
     1501 cgaacaccgc ctcgggccgt tggccgtgga cgatcgcgcg atacggcgag ccgcgatagc
     1561 gatacgcgag cacccacgcc ggcagcgcga cgcggtcggt cgtctgcgac tcgacgagac
     1621 aggagacgtg gatgttgcgc gtgcgccggc cgggcacgat cttctcgacg cggttcttcg
     1681 cgacggcttc gatcgagcgc gcgacttgct ggcgagcggc cgagcgctgc gcgtcgaagc
     1741 tctcgatcat cgacggctct accgtgccga cgccttcgac cgtcgataga tcgtagtagg
     1801 gaccgagcag cgagcactcg acgggcgtca ggccgcgcga tgcggggatc acgatgttgc
     1861 cgaaccgctc gtggatcacg ccggcgtgcg gcgcccagtc cgagcgttgc gagcccgcat
     1921 ccgagtcggc ggtccacgag acttccgcgg tcgcgttgac gatccatgcg gcccagcaca
     1981 gcggctgcag cgtctcgaac accgcctcgt ctttgagcgt cttcggcgcg aagaagccgc
     2041 ggcctcccag ccacgtgcgc agcgccgcct gcgcgagatc gagcgagacc gagaacggca
     2101 cgacgagctg cgcgacctcg atcggatcga cgggctgctc gaccttcatc gtcgcgcagc
     2161 agaacgcaca cgacggagcc tgcttgttcg cgtcgaacgc gaccgcggcg ccgcattcgg
     2221 tgcagcgcag gatctttgtc cgcactttct cgatcggcat cggtgcgacg ggcacgggca
     2281 gcgcgcacac cgagcaccgc aggtcgcctt cttcgagcgg gctctcgcaa cggctgcagc
     2341 ccggcgcagt ggcggtcgcg gcgctcatac gtgcggcctc gtcagcagga tcgccgcggc
     2401 gatcgcagcg agcacgaaga cgatcgtcgc gacgatcttg atccaagaca gcggcacctt
     2461 gccggcgatc tttccggtct ggccgttgat gacgaccttg agcggcggct tgtcctcgcg
     2521 atagcgcacc gcgaacaccc agacggggac gaagatcgga tcgagcgatt cccagctgac
     2581 gcgcgtgcgc cacgtcaggt cgctgtagct gtcgcccggc atgtagcggc gcagcttgcc
     2641 gccgatttgg tcgactgctt cgcgccggct cgacgtctcg cactcgctcg gcgggcgcgc
     2701 gaactcctcg gcgatccagc cgctgatgag cgcgggcgag tagcgccgca tctgcttcaa
     2761 gtcgtagggc tcgacgctct cgagctcggc attcgagagg cccgccgacg cgctcacgat
     2821 cacgtccgtc acgtagctga cgtgctgccc ggcaagcggc cgatactcgg tgcgcgtcac
     2881 cgtgcgcgtg cgcgtgtgcg accggccctc ggagtccgtc tcggtgtacg tctcggtctc
     2941 ggtgtagttc tcgccgatct gcgcggtgaa gtcgctgcgc gagacggcgc tgtacagata
     3001 cgacggcgcg tagatgccct tgatgtcctc gaccttggct tgcttcaggc gcgagtcggc
     3061 gaagatcgtg cggctgccaa gccagcgatc gagtgcgcga cgcgcgacct cgccatcacc
     3121 gacgaacgtg acgacgaact gcggatcggg ctggccttgc gcgggtggcc gctcgacgaa
     3181 gttcgggctt gcgcagaacg ggcacgtctc ggtgcgcaca ccctcgaatg cgagaccggc
     3241 accgcagttc tgacacgcga acgtggtcat ggatcagttg cagggtaacg cacctttgag
     3301 cacgcggacg tcgtcgatgg cgccttggaa gaacgcacca gcgggcagcc acggtccgtt
     3361 cttgccgacg aagagcgggt aatcggactg catcgcgccc gcggcgagga cgtagctgcc
     3421 aaggcgaaca ccgtcgacgc aaatgcgcac ggcggtgcca gcctggatca cgcggacgaa
     3481 gtgccagccg tttgcgggcg cgggatacgg cgcgtcgaag tagtgatgct cgagggtgtt
     3541 cgcatcgacg cacgtctgtg cgccgatatt gccgccgccc atcagcttgt agaccgcgag
     3601 gccgcctggc ttgtccagat cgtcgtcgga gacgagcacc gcgttctcgg tcccttccat
     3661 gtcctggcgg acccagaact gcgtcgtcgt atcgcccatg cgatccatga tgtcgccgcc
     3721 gcgatagtag ttctcgaacg tcagggacgc ggccatgccc atctcgggga atggacctgc
     3781 ggtctgcgtc ggatcgacat cgttgaacga gttgtccatg ttgtagtgcc gagagagcgc
     3841 cgcgccgccg cacgggttcg ggatcatgtt gcccgtcgcc gcctcgttaa actgcagcgc
     3901 catctgacac tcttgcggga ataccgtcgt cgtgccgttg atgaacagct gcacgccgat
     3961 gtcttgcgcg cccgcgttgg taggtgccag cgcgagcaag aaccgctcgc cggcaagcgc
     4021 gtcgagagtg atcgacgtct cgagcatgtt gccctgtgtc gccgtgaacg tgtagagcga
     4081 gtcctcgcgc gcgttgcgat agagccgcac catgtggtct ccaccgttct cgatatagac
     4141 gcgcagcgag atcttgatga cctggttcga ggacgacgag aactcgagcg ccgggtcggc
     4201 ggcatcggac ttgcccgccg tcgaaacgag gagcgcgccc ggcaacatgg tgcacgcggc
     4261 ggcactcgag ccggcgcacg gcgagaactt gttcatcgtg ttcgcgccga cgtagctggt
     4321 tccggccgcg gtcatcggcg tccacgtgcg atcgcgatga tcgtcgaggt agcgccactt
     4381 cgtacccgtc ttgccaagat cggcgcctgc atctccgagg cacatcgcgc cgcatgatga
     4441 ctgcaccatc acatcgccgt tgtcgttcgc gtcgcccgcg atgtcgtcgc caccgccgtg
     4501 atcggcgaac agcgacagct tgcaaccggt gaggagaagc gttgcgagga cgatgcggct
     4561 cagaaccaca gctcacctcc gacgaatgca ggcagcgtcg tgccgtgcgt cttaccagcg
     4621 aagctcgcga atgactcgag acgaatcgag aatgaaggca ttgggcgata gcggaggccg
     4681 aggccgacgg cgccggcgac gaacgtctcc gacatgccgt cgtcggtgat cgatatcggc
     4741 atcgcagccg cgaggtgaac gctcactgca ttgccggcga gccgcacgcg gacgctcggc
     4801 atcaccgcgt atgcaacgac gacagcatcg acgccgacct ggatccgatc attgatcgcg
     4861 acaccgaggc cgagcgtcgg tgcgccggtg tcacctgctt cgagcaagtt cgtgccaaac
     4921 gctgccccga tcgagaagcg cttcgtcgtc cgcgtgggcg cgacgggctc ctcgatcttg
     4981 tctctcacgg ccggctcttc gatcaccgtc gccttcgtct cggcgggcgt ttcgcccagc
     5041 gtgagcgtga cctcgacttc gtcgcccgat gcgacgcgca cggtgcgttc cgcgtcctta
     5101 tggccaaccg cgcgcgcggt cagcttgtgc tcgccgggtg gcaagatgat cggctcctcg
     5161 aacggtgcgg tgccgagcga gatgccgtcg acgaggagct cggtgccggc cccggagctg
     5221 acgctgaccg agacgcgggc gatgcgtgcg agcacgccgt cgagctcgct ctgcgcggtc
     5281 ttgatcttgg ctgaagacac cttcgccgcc ggagcctctt tgatgaagcg gcgatagaac
     5341 ttcacggcct cgccgtagtg cgagagctcg cgatgacacg tcgcgatgtt gtagagcacg
     5401 agtgggtgcg gcgcgatctc gtacgcgcgc tcgaactcgg caagtgcctc gccgtacttc
     5461 gcctctttga acagcgcgac gccggccttg aagtgcttgt cggcttcctt ctgttcgccc
     5521 gcagcgggct tgtccttatt gggaccggcg agtgccaccg ccgacgacgc gagcacaacg
     5581 atgaaggcta gtttcttcat ttgtaggggc tatcgacttc gatcttgtcg cgcttgccgt
     5641 tggtcttgtg accaccgacc ggcgctttga cttgcttcgc gagcgcgacc gagatgcgct
     5701 ggctttcgtc gaagcgaacc ttctcgctgt gcgctacgta gccgggcgcg gtcacgctga
     5761 gctcgtgctc gctgtcgtcc ttgggctgcg tgatatcgcc ggtgaccggc ttgccgtcga
     5821 gcttgatcgt cgcggtcgcg ggtgtgacgg agagcttcag cgtaacggtc gcgggttcgg
     5881 cgggcggcgg gggcgacgcg accggcggag ctggcgttgg cgcgggcggc gtgggagccg
     5941 cgctcggcga cgtatcgaca ggcggcgtag gtgccggctc gtttgcggcg gtcttcgtcg
     6001 aaccgtcgcc gccggacatc gcgatgaacg cagccgcgcc accgccgatg agcacggccg
     6061 cgatgatgat caaaggcagc ttcgagcgct tcgcgggttg gcggatgtag ctcggtgcga
     6121 gcagcgtcgg gtcggtgccc tgattgatcg gcgcttgcac gccgggaccg gtgcctggat
     6181 gcgagccata tcccggctgc gagccatagc ccggctgcga gccatagccc ggctgcgacg
     6241 agctgtaggg catcggcgtc gcgcgccacg ggatgcccgc gttcgacagc cgctcgatca
     6301 tgtgctcgcg ataaccgggc cggcagaacg cgagcagcgc atgctcgagc tcgcttgcgc
     6361 tcgtccagcg cagggatgcg tccgtcgcca tcgcgcgctc gatgatcgct tcgagctcga
     6421 ccgggatctc gggccggcgt tgccggaggc gcgcgtactc gccgctcatc acgcggaaca
     6481 tgagctggtt gtagttgtcg cccttgatcg ggacctcgcc gaccagcatc tcgtagagga
     6541 tcacgccgat cgagtagatg tccgacgcgt gcgagacctc gctgtcaccg cgcgcttgct
     6601 ccggcgacat gtaatagggc gtgcccatca cgacgccggt attcgtcagg cggagctcgg
     6661 cttcgaggcc ctgcgcgcgc gagatcccga aatcgaggag cttcaccatc tcgccgcggc
     6721 tctgccgaac gaggaacacg ttgtccggct tcagatcacg gtggacgatc cccgctgcat
     6781 gcgcggccgc gagcggcgac aagatcgcgc agccgagctc gcacgccagc tcgacaggca
     6841 gcggccccgt cgccttgagg cggccggcga gcgactcgcc ctcgagcagc tccatgacga
     6901 gaaacgcctg gccatccgtg ctcgtgccga ggtcggtgac ctgcgcgata ccctcgtggc
     6961 cgatctgcga tgcggtgatc gcttcgcgct gaaaccgctg cagcatcgcg ggcgacgtga
     7021 gctgcggatt gagcaccttg atcgcgacct tcttgcgaag cgcgacgtgc gtcccgacat
     7081 agaccgcgcc catacctccg accgcgagca agctctcgat gcggtacttg gcatcgagca
     7141 tcgtgcccgg gggcaactga cgttgtccgg ccgctgcctc cacctagatc aaggataagc
     7201 tacacggcgg gtgaattcac gccgacatgt gcgggctgtg tgtgtgctgg cgacggttct
     7261 cgcggtgtgc cccgcggggg cgcctgcgga ggccagcccg ctggacatcg tcggattggg
     7321 ctctcgacgt gcgggcgagg ccaacaccgg gctcgcctcc gtcgacgatg cgagcgcgct
     7381 ctactacaac ccggccggcc tcgttgcgca cgcggcgtgc cgcctcgaac aagaacccga
     7441 gggcgactac gacgaatcgc cggatccgat ctgcgtcgaa cgagatcgcg gcgacttcat
     7501 cattggcgtc accggcgcgt actcgcatct cgcgatcaac aaagtccgcg cgccattcaa
     7561 cgacgcaggt ggcgcccagc tcgcagcccg agcacgcatc accgagcgct tcgcactggc
     7621 gttcgcgttc cacggtccct cgcaaggcgg cgtgcacgcc gacgttcggg atcccgagta
     7681 cccgctctac ccgtactacc gcgaccggct ctcgcgcccc gtcctcctcg tcggcgcggc
     7741 gacctcgctg tccgacgccg cgcgcgcagg tgccgctctc gatctcggcg ccggaacgtc
     7801 gcatcccaaa gcgatcgccg gcgtcacgct ccgcgtctcc ccctcgctgc aggccggcgt
     7861 cgtgtggcga cagcgcttcg cgatcatcga cgacaccacg ggcgcccgcg tccactacac
     7921 gccgcacacg gtcctcgccg gcctgacgtt cgcgaacgat gcgatccgcg catccgtcga
     7981 cgccgggttt gctcactggt ctgcgtataa aggcccgttt ggctccgatt cgaccgtccg
     8041 cgttccgtac aagagcaccg gccaactgcg cttgggtctc gaatcgaata aagacgtggg
     8101 cgtctcgttc cgcgtcggct atgcgttcga atccgcggcc gtccctccga cgcagccgac
     8161 gtcgaacctc ctcgacggct cgaagcaaac tgtcgcgggc ggcatgagct acggccaggg
     8221 caacattcgc gtcgacgcgt acgtcggcct gcaaatcgtc ggctcgcgct cgatgacgga
     8281 ccccgacgac accacgctga aatcgggcgg cgagatcttt accgccggac tgtcgctcgc
     8341 cgtcgggtac tgacgcgtac ctattcccac cgtgcgctgc cgctcgggag tgaaagcgag
     8401 agcgtcgtta gatcgccgat ttttggactc tcctcgctac acacgcgacg atctcgaaac
     8461 acagtgctga acgcccgcgt tgtttccacg aggtttcaga agtcatgatc gccgtcattg
     8521 gtaacgttct cgccggactc ggcgtcgtct cgctcttcac cgcaaccttg cgactgacga
     8581 agcccggcaa gtagtagact ggtggccgtg gtggccaccg tcatcccgcg cgtcgcgcgg
     8641 cctccggcag ccgacgagct aacgctcgcc gacacggact cggtgcgcct gatgctgcgc
     8701 ggtgactcca tcatcgactg gcatcgcctc aacttcgccg accacgccga agtcgaccgc
     8761 ttcctccgcc tcaacgaatt cgatcccgaa tccgatcccg aaatggatcg cttcgaggac
     8821 atccgcgcgg acgcggtcga ctacctcgcg cgtgcgttcg cgatggccat ccccgacgaa
     8881 gtcgccgccg acctccccgc tcgcgacctg tttctcgtcg cctctagctc gggcccgcac
     8941 caaaagtggg cgtgcgtcgt gctgaaggtg atgcacatca tccatcacat caacggccgc
     9001 caagccctcc tcaaagtctc ggtctcggac gaaatcatct tccgcgagat cgagatgaag
     9061 gtgctgcaag tcgtcgagca gctgcgcgcc gcgggtgccc cgctcaccga gttcgaatgg
     9121 tcgcgaaagc cgcgcgactc gcaaatcacg aagctgctcg caaaacgctc gacgctcgcc
     9181 gccagcatct acgacaagct gcgcttccgc ctgatcgtcc cgcactacgc agacatcgtg
     9241 ccgatgctcg cgacgctgac gcggcagctc atcccgttca actacgtagt gcccggcgaa
     9301 tcggtcaatc agctcgttga cctctcgcat ttctctcccg actcgtccgg catcatgcgc
     9361 gcccccgacg caatgccgtt caacgagttc tccggccccg aataccgcat catcaatttc
     9421 gttgccgacc tgccgctacg cctcgagcgc ctgatcccga agaacgagca acaccccgag
     9481 ctctcgcacg tcgtgttcgt cctcaccgag tttcaggtct gcgacaaggc gacagcccag
     9541 cgcaacgaat cgggcgcgtc gagtcacgag gcgtacaagc agcgccagca cgagcgcgtg
     9601 cgcatgcgcc tcttccgcga caaagacgat ccgattcccg agtagtcagg tggcgacgcg
     9661 cgtcgcgccc ggccacggat cgtcgacggc ggcgggataa tcgaaccaca ggcgcgcgct
     9721 tgcgacatcg cacatatgcg ccatcgcggc cagcggcccc atgcgccgtt ccttgttcgt
     9781 gtagtccgag agctcgcctt ctgcataccg cagcccgttt tcgtgctcgg cggtgagcgc
     9841 gacgccataa cgctcgagca tctcgaggcg gaaggcgtga tgcgcttctt tgccctgcat
     9901 gttcgccttg tgatggagct gcccgtcggc gccgagctcg tacttccacg gcttctcgat
     9961 gtcgtggaga aacacgacaa gcaggatgtc ggagcgcgag aatggcagcg gacgcagagc
    10021 cgagagccgg tcgtagagga ccagcgcgat gttcatgacc tcggtgatgt gatcgaggta
    10081 gccgccgcgc cacgcctgat ggttgttcgt ggatccctgg accgtcgcga acaacgcgcg
    10141 gttgtcggcg acgatccgcg cacaggcgct gcgattgggt tcatcgatcc gcgcgatgag
    10201 tgcgtcgacg gactcgtagg gcgtcatggc acgagtgtgc cacgtgcatg cggcggtgcg
    10261 atctacttcg cgccgacgga cagctcgatg tgtttctgcc cgagcagctc accggtcgcg
    10321 cggccgagcg ccgctactgc cttcgtgtga tcgagcacgg cgcccacttc cgcgatctgc
    10381 gcggcggcca gctcctcttg cctctgcgcc acgacgacct gcgacgacga gccgttgagg
    10441 aacttgcgct gctcgacttc gagcgtcttc tcggcgatct ggcggaagtg cgcagccgca
    10501 gcgacttgcc tcgcggcgtt gtcctggttg cggaccgctt cgcgaactgc gagccagatc
    10561 tgctgcacga gctgctcgcg ccgcgctgtc gcgatctcgt gccgcgttcg ctcgatttca
    10621 gcttgcgcgg aggtcgcgcg gcgcagcggc gtccacgtga agttgacgaa cacattccac
    10681 ccgcgcgctt cgaagccacc gagttggctc agcgctccgc tgtattgccc gtcttggccg
    10741 acgaccgaac cggagaggcc gaggtcgatc tgcggcagct tgtcattgtc cgcttttcgg
    10801 acggagagct cggaggcgcg cagctcgaga tcgatttgct cgatttctgg gcggtgtttc
    10861 agcgcggtct cgagcgcttg tgcggcgggg agcgcgtgcg gctcgaagtg cggcacgtcg
    10921 acggggagga tcggcttcgt ccactcgtcg cgcggcatgt tgatgatcga gcggagctgg
    10981 tcccacgacg cttcgatcag ttgttcggct tgcacgacct gcagctggcg ctgcgcgagc
    11041 gtggattcgg ccgagatcaa atcggccggt gggatcgcgc ctgcagcgat ctggcgcttc
    11101 gtgaggtcca gttgctcttc cgcgcgcttt tgcgagcggc gctgcaggtc gtagttgtag
    11161 agagcgcgga ggacttccca gtaggcagct tccgtgcgct cgacgacttc ggcaaccacg
    11221 acggagagct ggcggcgttc gcgctccgag ctgatgcgag cttggaggac ggagagctgc
    11281 ggcaggtcga ggtcgaacga gaagccgcga aggaggggct ggcgtatgcc gaccgtgagc
    11341 gcgctgcggt agttgatcgg ctcgacggcg gtaccgagcg acgagtcgga gcgcgacgag
    11401 gtccaggtcg cggagagctg catgccggtg gcgaggcgct ggttgatgct gaggtcccag
    11461 cgatcgtcgg tgaagcggag gatttcgccc gccatgcctt cttgggcgga ggatggtggc
    11521 gtgcgcgagc tgccgtgggc gtaggtggcg tcgacttccg gctcgaacag gccgcgcgca
    11581 acgtcgacgc cgagctctgc gatatgcgtc tcgcgtcgtt cgagcgtcac gcctaggttt
    11641 tgccgaaccg ccatctgcag cgcttgcggc agatcgagac ggagcgtgtt cgcggcgtcg
    11701 accgtgtcag gaagcggcgg cgtggcgctc aagaagtccg gcggcacgta cggcgcggca
    11761 ggcgcagcat catctgcacg cgcagacgac gcagcagtca cgagagacag gacagcgagc
    11821 caccggccaa gcatcgaggg attatgatga gaccgcatgg gcgaatccaa cgatacgcgt
    11881 gcgcctgcga atgcggacac ggttgatgcc aatacggcac tcacagaggc cgcagagcgc
    11941 gaagctgaca atttgccgtt cgagctgcct ccgcccggct accagctcgg cacgatgatc
    12001 ggcaaaggcg gcatgggcga ggtgatggcc gcacacgatc agcggatcgg gcgcgacgtc
    12061 gcgttcaagc gcatgcgcaa tgccgatgcg agcggggacg cattggcgag gtttctgcgc
    12121 gaagcgcgca ttcaggcgcg actcgaccat cccgcggtcg tgcccgtcta cgagctcggc
    12181 aaggatgccg aggggcggcc gttcttcacg atgaagcggc tgacgggcaa gacattgacg
    12241 gcgcatctcg cggacaagga cgcgccgagg cagcctctgt tgcgcgcgtt tgcggaagtc
    12301 tgtttggcgg tggagctcgc gcattcgaag ggtgtcattc atcgtgatct caaaccgtcg
    12361 aacatcatgt tgggtgattt cggcgaggtg tacgtgctcg attggggaat cgcgcgtgtg
    12421 atgggtggcc ggcgccgtgc gccgacgaac ggcatgcaag ccgatgacat cgactcggtg
    12481 ccgggcgaag gaacgatgac gggagacatg cttgggacgc ccgggtacat gtcgcccgag
    12541 caaatgcgag gccaactcga tgtcgggccc aaggcggacg tctactcgct cggatcgatc
    12601 ttgttcgaga tcctgaccgg cgagccgctg catccgcgtg ggcacgcatc ggtcgcgagc
    12661 acgctgacga tggaggaagc gtcgcccgcg cagcgcaagc ccgagcgcaa tatcgcgccg
    12721 gagctcgatg cggcatgtgc ctcgacgctc gtcgccgatc cgcagaagcg accgactgcg
    12781 cgcgagctcg ccgagaagat cacgcgctat ctcgacggtg accgcgatgt cgagagacgc
    12841 cgcgcgatcg cagcggagaa tctggcgacc gcgcgcacgt tgctggctga gggcgacgcc
    12901 ggcaaacggg ccgaggcgat gcgggccgcg ggacgcgcac tcgcgctgga tcccgaatcg
    12961 aaggacgcgg cgcagctcgt ctcgagcatg atcgtcgaag cgcccgcgca gaacccgcca
    13021 gagctcgtgg cggagatcga agcgaacgag atccgtgggg ccaagacgcg cgcgctgaag
    13081 ggaatgatcg gcctgctcac gctgtcgtcg ctgtctgtgc tcgtgttgtt tctcgaggtg
    13141 cggagctggg cgtgggtcat cgcgatctgg gtgacgctcg cgtcgctcgc cggtctcatg
    13201 tggacgacgt atcgcaccgg ccgagtctac ggcgtcgcga tcctgctgta tggagtcgcg
    13261 ctcgcgatcg agttctcgcg catcgcgagc ccgctcatcc tcgttccggc gctgatgtgc
    13321 ggcgcgatgc tgcccttctc caacattcca cgcatcaatg atcggccaat cgtcctctac
    13381 gtctggtgcc tggccgtcgc gatggtgccg tcgctgctcg agctcgtcgg cgtgctcacg
    13441 cccacgctgc gaatcggcga gggcaacttc gtcgagctct actcggaagt attcgtgcac
    13501 tcgccgcgtg cgggcatcgt cagcctcgtc atcgcgaacg ccgtgctgct tctcaccgtc
    13561 gcgcgctacg cgatcgccat cggccgcgac cgtcgcgacg tccagggcaa gctgacgaat
    13621 caggcctggc accttagaaa actgcttccc tgaccagata tcctcgacgg gatggtagac
    13681 gacgaccacc gcgggacacg cctcgatcca ggtgcgccca cggtcgacgg cagcatggcc
    13741 ggtgcgggca atgcgccgac ggagctgccg agcgcgaccg acgagacgat cgttccgtcg
    13801 tggggcgcga gcgagatcgc agcgctgccc gagcccggct atcaaatcgg cgagctcatc
    13861 gggcgcggcg gcatgggcga ggtcgtcgcg gcgcaagatc aacgcatcgg ccgtgaggtc
    13921 gcggtcaaac gcatccgctc gctcgagcca tccgcggacg ctgtgatgcg ctttttgcgc
    13981 gaagcacgca tccaggcgcg cctcgatcac cccgccatcg tgcctgtcta cgagctcggc
    14041 accgacgcgg aaggccgtcc gtacttcacg atgaagcggc tcgctggagt cacgctcgcg
    14101 aagaagctgc agcagcaagg cggccccgtg cagccgctgc tgcgcgcgtt cgtcgatgtc
    14161 tgcctcgcga tccagctcgc gcactcgcgc ggcgtcgtgc atcgcgacct caagccgtcg
    14221 aacatcatgc tcggcgacta cggcgaggtg tacgtcatcg actggggcgt cgcacgcgtg
    14281 ctcaccgata cgaagcgcac gacgggccct gccctcgccg tcgatcccga cgacgacacg
    14341 accgccggct cgatcctcgg cacgcccggc tatatggcgc ccgagcaagt gcgtggcgcc
    14401 gaggctggca cgaaagcgga tgtgtacgcg ctcggcgcga tcctgttcga gatcctcgcg
    14461 ggcgagccac tgcacccgcg cggtgaagct gcgctcgcca gcacgctgac gtcgccgcaa
    14521 gaggcaccca cgcgccgcgt tgcagagcgg cggcagattc ctcccgagct cgacggtgtc
    14581 tgcttcgacg cgcttgccga ggagccggcc gccagaccga gcgcgcgcga gcttgccgac
    14641 cgcatgcagg cgtatctcga cggtgatcgc gacgtcgaac gccgccggac gatggccgca
    14701 cagcaactcg catccgcgcg cgaagctctc gacacgaatg accgagcgac tgcgatccgt
    14761 cgggccgggc gggcactcgc gctcgatcca gagtccgccg atgctgcgac gctcgtgacc
    14821 tcacttctcc tcgagccgcc cgatgagatg ccgaaggatc tcgtcgagca acttgccgcc
    14881 gatgacaaag tcttcaacag ctcgcgcagc cggcaaggcg cgtggaccta tctgtcgctg
    14941 ttctcgttct ggctcgtcat cccgttcatg ggcgtgaaga gctggacggc gctgatcgcg
    15001 ttctacgggc tcctcggcct cggcgcagcg ctcgcctggc gcaactcgaa tcgcggctac
    15061 tcgagcatcc ctgtcacgtt cgtgatgacg atgctcgtcg cgctgatgtt cacgcgcatc
    15121 tcggggccat tcattctcac gcccgtcgtg atctgcgcgg cgctcatgca gctcgcagcg
    15181 atccggcaag tcgcagagag actgtggctt gtcgtgagct gggtcgtgct cgcggtgatg
    15241 ctgccgttcg cgctcgagtg gacgcacctg atcgacccga cgtacgaagt gaacaaaggc
    15301 ctcatcatct cgacgtcgaa gatcttcgac atgcgcaccg gcatcgacga agccactctc
    15361 gtgatctcga atctgctctt catcctcgcc gcgggcatgg tcgcggtgtt catcagccgt
    15421 cgccgcatga aggcgcagcg ccagctccag atccaagcgt ggcacctgcg gcagctactg
    15481 ccgacggcga agcgctggca aacccaaccg cgcggtaggc gtacactctt gggatgagcc
    15541 ggccgccggg cggggacgat cccaccgcca cacccgacct cgatacagcg cccgcgtcgc
    15601 atgattcgat gcccgtactg ccggagcttc ccggctacgt acccggcgaa acgattggcc
    15661 gtggcggaat gggcgaagtc attgccgcac gcgacgtgac tctcgatcgt gaagtcgcgc
    15721 tcaagcgcat gaagatgcaa tcgccgacgt cggatgcgat cgcgcgcttc atgcgcgagg
    15781 cgaagatcca agcgcgcctc gatcacccgt ccatcgtgcc ggtgcacgag atgggcaccg
    15841 acgtgaacgg caacccctac ttcacgatga agcgcctctc cggccgcacg ctcgcgcagg
    15901 tgctggagtc acgcacgctg tcactacaag cgctgctacg cgcatttgtc gacgtgtgct
    15961 tcgccgtcga gcgcgcgcat gcggcggatg tcgtgcaccg cgatctcaag ccgtcgaacg
    16021 tgatgctcgg cgacttcaac gacgtctacg tcatcgattg gggcgtcgcc cgcgtcatgc
    16081 gaacgaagcg cacgtcgtcg atggctgctg ccgtcgatat cgactcgctt cccgtcgacg
    16141 aaacaggggt cggcgtcatg ctcggcacgc ccggattcat ggcgcccgag cagatgaagg
    16201 gcgacgacgt ctcgccggct gccgatgtct actcgctcgg cgcgctgctg ttcgagatgc
    16261 tggcgggcga gccgctgcac ccgcccggca agcaagccat ctcgcacacg ctcgccagtc
    16321 cgacggagtc accggcgaag cgcaagcccg agcgcgctgt gccgcccgag ctcgacgagc
    16381 tttgcgtcgc tgctctcgac gaagatccag cgaagcgacc aaccgcgcgt gagctcggcg
    16441 accgcgtgca gcgctacctc gatggcgacc gcgatctcga acgccgccgc accctcgccg
    16501 cggagcagct cgcgaaagcg cgcaagctcg tgacggaacc cgcacgtcgc accgagggcg
    16561 ggcagatggc ggcacgcgcg ctcgcactcg atccggaatc gaaggaagcc gcagagctcg
    16621 tctcgcgcct gatcctcgag ccgccgaagg aattgccgcc cgcgctcgtc gaatcgctcg
    16681 aggattccga acgcaagctc aacgcccagc gcggcaggag cgcgatgatc gcgtttctca
    16741 cgctctggct cgtgctgccc gtcttcatca tcttccagca catcaaggac tggacgcagc
    16801 tcgcgctcct ctacgcggtt gtcacgctga tggcgatcct gtcgtgggag aacggccgca
    16861 ccggtcgaac gccaacgtgg atgaccgcgt tcggtaactt cgccgtcgcc gtgatgttct
    16921 cgcggctgac cgggtcgttc gtgctcaccg tcggcctcgt cgcgggccaa acgctcgcgt
    16981 tgtcgtcgcg gccatggttc gtgcacaacc gcaaatggat gatcgcgtgg atcgtcgtct
    17041 cgctcctcac gccgttcacg ctcgagtggt tcggcctcat cgaatcgacg tggcacatgg
    17101 cgccgggcgg cctctacgtc accgggacga tcctcgacac cacacatacg cgggacgtcg
    17161 tcgtgctcgc gttctcgcaa tgcgcgctcg cggtcgtcgt tggcctgttc ggaatgtcca
    17221 tcacccgcgc gcgggaggaa gcgcaacgac gtgcgcatat ccaggcttgg catctccagc
    17281 agctgctccc cggcgccaac aaggacatca ccggctcggg ttcggcggac aaattggcaa
    17341 gcacgcgtta ggcgatttgc tgcaacctgc ggcaagtccg gcttttcccg agaaacgtga
    17401 cccgcggttc tttggcacgt ctggtgtacc ctgccccggc ggtggctggc cctctgatcg
    17461 agctcgttga cgtcgctcgc tattaccaga tgggtgacga gcggatcgcc gcactcgatg
    17521 gcgtgagctt caccgtgcat gagggagaga tggtcgccat cgtggggacc tcgggctccg
    17581 gcaagtcgac gctgctcaac atcctcggca tgctcgacac accatccggc ggccgctacc
    17641 gccttggtgg ccgcgacgtc caggacct
//
LOCUS       DATLBZ010000604         8529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7196441, whole genome shotgun sequence.
ACCESSION   DATLBZ010000604 DATLBZ010000000
VERSION     DATLBZ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7196441"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            575..1462
                     /locus_tag="VMZ53_29630"
     CDS             575..1462
                     /locus_tag="VMZ53_29630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HUS32712.1"
                     /translation="MEIDLHQLDLRHLDLRIVDGDRRRRLIGSLAEIGQQVPVVVIAD
                     GDRFVLIDGYLRVDALARLQRDTAIATTWSVPEIDALVHHRHLAVARRTALEDAWLLG
                     RLREHGLSMDQLARRLCRSKSWVSHRLGLVDALAAATQDRVRAGTVPPHGAMRYLVPF
                     ARANKTQCERMLAQIGDTRVSDRELSALYTAWKRADRAGKQRIVDDPLLFLKAWAAAQ
                     AARSDEDDAAGSLLKDLTTLAAIAWRANRRAKDDGGPVTAGVTRAHRAASDAFAALGA
                     TLEEMHAGPDHSTSHPDPS"
     gene            1527..2882
                     /locus_tag="VMZ53_29635"
     CDS             1527..2882
                     /locus_tag="VMZ53_29635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32713.1"
                     /translation="MLADGRAAPPPVARAEQLEPVRDEILALHGSCKGNFVRVHEELA
                     ARHGVTVSYPALTAFCRKHGLGHAPPLPKGSYDDVLTPGLEMQHDTSPHDVSIGGKLL
                     RVNTASLVLAYSRRLFFQFSPRFTRFDCKVFLDDAVAYHDGACERCMIDNTHVVVLHG
                     TGRDMVPVPEMEAFGRRLGFSFIAHAIGDADRKARVEGPFSFIENNFLAGRKFADFAD
                     ANRQAREWCDKVNAKFRRSLSASSNELYAVERPKMRRLPAWRPEIYQLHQRVVDLEGY
                     IHVDGHIYSVPYQLIGKPVEVRESKDKIRVFRGPREVAVHDKVISTLRNQRRTSAAHR
                     PPRGQRAAPPPALPEERELADAGAPFAHYAAAIKKRGGSRWPAALRRLAQMRRDYPVA
                     QLEAAVRSAMHYGLYDLDRLERMILKQVATDFFVLPANRDEVIDEVDGSSDDLEDGDE
                     G"
     gene            2872..3597
                     /gene="istB"
                     /locus_tag="VMZ53_29640"
     CDS             2872..3597
                     /gene="istB"
                     /locus_tag="VMZ53_29640"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HUS32714.1"
                     /translation="MKDDLDQLLRSLHLRKIAEIFDEEVKRAEKADTSYQEFVARLMR
                     AQWQAKQEAALAWRIDRAGLPEEWTLESFPFKRQPGVNARQIRTFAELEFVSKAENIV
                     FIGPTGVGKTGLASALLLKALQNGHRGVFMRAQDLFDEMYASIADRSTRRLLNRLAKV
                     DVLVVDEMGYLNLRPEQTNIFFKLMEERYRQRATIITTNLDYAEWGNFLGNKALVDAL
                     LSRLRHQCHTVTIDGPSLRDPTG"
     gene            complement(3754..5313)
                     /gene="istA"
                     /locus_tag="VMZ53_29645"
     CDS             complement(3754..5313)
                     /gene="istA"
                     /locus_tag="VMZ53_29645"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HUS32715.1"
                     /translation="MNEARRQLVYEVVRRAARGESKRGIARALRIAPRTVKKILLRED
                     VRREQGESALDRELPSRRAPRGSKLDAFDDKITAWLEQYPDLTATRLHEKLAGEGFTG
                     AYTIVREHLKAWRAQHATPKRADLRVETAPGHQGQFDWSPFKLENGEIENVWSYTLAW
                     SRGRSFASEDNQRQTTILRRLHDSFTQLGGVPHESVTDTMAGVVDGWECNQPILNIRF
                     VDFAAYYRFAVHVSPRRYPKYKGKVERPFRYVDENFCNGRTFRSREEFREGLAWWRDT
                     HAMQRPHPVTGRTLAEMLEEERPYLQPLPRVPYDTRDVVQRHVDSTAHILYETNLYPV
                     PDEHIGQLVYVCVGSDRLEVFDRGVHAITELERMPDGAGLISLTERKRRGRYDVTLLQ
                     ARFAAWGPSAEDFANKLRRRKRTPGPELDHILKLQVTWSVDDIVGAIEHATKYDAFDA
                     RAIERILEARFRPRRLAEQIAEVTRNQIRDAMREHPIEQRALSSYETLRLGDGFLQER
                     REEDARDARQN"
     gene            complement(5310..6224)
                     /locus_tag="VMZ53_29650"
     CDS             complement(5310..6224)
                     /locus_tag="VMZ53_29650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HUS32716.1"
                     /translation="MTDKKESRDEVAIGELDQRYASLRLAAPDELGRVRASVERMGIL
                     SPVLVATAVEATRIVLVDGFKRVRIATDRGDSVMWIRRTALDAAGAKVAMVAANAGHD
                     GLSDLEEAWIVRSLCRDHGLTQVEVGKALRRDKSWVSRRLMLAERLENALQDDIRLGL
                     LAPSVARELARLPRGNQVPMAATIRAHELTSKQAHRIVTELLRTADPTARDEVLADPL
                     RYLHAIELPATSVEDPRLGKGGNDVRKSLLAVGGAAERLVRIATRHAPVGLVGDDARI
                     LGPLVARTLLASRETTALLEQLATSSGS"
     gene            7410..7601
                     /locus_tag="VMZ53_29655"
     CDS             7410..7601
                     /locus_tag="VMZ53_29655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32717.1"
                     /translation="MSRGRRSYNLIDRSKRLRKAKDIPDLRRAYDACGGDLDIAAAEL
                     EVSRRSLEQRIAELGWDAG"
     gene            complement(7706..>8529)
                     /locus_tag="VMZ53_29660"
     CDS             complement(7706..>8529)
                     /locus_tag="VMZ53_29660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32718.1"
                     /translation="RAHLQTQLAERAEGNGVISIDETRAIRIADGRSLHDVLDATGVL
                     EVVPDLQLTAPLGVDDWARTAPLVTFVQLDDTAPLTYIAADGTSSTLPADHAPTGPVL
                     AIGWNERSGIDDDVSVDNTTSSPSVTSSPFYLRALLFTDAMESMFAGDPEMYIKCSSY
                     SVDGVYQRSFAREWVDQANDTIHWYTLNRYVATLGANEARVTCDVKESDGSSGDDLVG
                     TVTFLRDGLVDGTGTYCIFDNGGNAHVGASDVAASTTDSPYGPIPRKCSNTYLWE"
BASE COUNT         1386 a         2903 c         2751 g         1489 t
ORIGIN      
        1 ccgcagcgct cgcgaaaaat gacccgcctc tgctcgtcaa aaatgaccca ccacacttcg
       61 gtggaatacg tcggcgatcg tcgcgggcgt tgtgcggaag aaacgcccgt gttcgatctg
      121 cggctgctgg ttctcgactg acattcgcgt aggtcagcgt cagcgcacct gtgggagcga
      181 tgagtgtcag cgcgagttgc atcgacgagc ggatcgacgg tggcgcgcca agaatcccag
      241 ctacgacaga gagcgccgca tgggggcgca gctcgagaag gcgcgagcgt cggccgcggc
      301 gaaggcggcc ggggttccgg tcgatagcac cgcggtgccg ccgtcaccgc gcgcacctgc
      361 gccgacctcc ggattgccct gggacctcgt gcaagaggag atgggggtgg aagctcgcgt
      421 aatcatttcg ggagtgacgg gagtcctcgg tgtgtacgtg caagatgaga tacgtcgtca
      481 agtccgtgat gtcattccgg gagtagcggg agttctcgga ggtagcgctc aagacgagat
      541 ggctccggga gggtagaggc agcagcgtgg cggcttggag atcgatctgc atcagctcga
      601 cctgcgccac ctcgatctac gaatcgtcga cggcgatcgg cgtcgccgac tgatcggttc
      661 gctcgccgag atcgggcagc aggtgccggt ggtggtgatc gccgatggcg atcggttcgt
      721 gctgatcgac ggctacctcc gcgtcgacgc gcttgcacgc ctccagcgcg acacggcgat
      781 cgcgacgacg tggtcggtgc ccgaaatcga cgcgctcgtt catcaccggc acctggcggt
      841 cgcgcgtcgc accgcgctcg aggacgcgtg gctgctcggc cgactgcgcg agcacgggct
      901 gtcgatggat cagctcgcgc gtcggctctg ccgctcgaaa agttgggtat cgcatcgact
      961 gggcctcgtc gacgcgctcg ctgcggccac gcaagatcgc gttcgcgcgg ggaccgtgcc
     1021 gccgcacggc gcgatgcggt atctggtgcc gtttgcgcgc gcaaacaaga cccagtgcga
     1081 gcggatgctc gcgcagatcg gcgacacccg cgtctccgat cgcgagctca gcgcgctgta
     1141 caccgcatgg aagcgcgccg accgcgccgg caagcagcgc atcgtcgacg atccactgct
     1201 gttcttgaaa gcgtgggcag cggcgcaggc agcgcgttcc gacgaggacg acgccgccgg
     1261 ctcgctgctc aaggatctga cgacgctcgc cgcgatcgcg tggcgagcga atcgtcgcgc
     1321 caaagacgac ggcggtcccg tgaccgccgg ggtgacccgc gcccaccgcg ccgcgagcga
     1381 tgcgttcgcg gcactcggcg ccactctcga ggagatgcat gctggaccag accactcgac
     1441 aagccatcct gacccttcat gagcgaggtc gcggcaaacg cgcgatcgct aaagcgctcg
     1501 gcatctcgcg caatagcgtg cgcgaggtgc tcgctgacgg ccgcgccgcg ccgccgccag
     1561 tcgctcgggc cgagcagctc gagcccgtgc gcgacgagat cctcgcgctg catgggagct
     1621 gcaagggcaa tttcgtgcga gtgcacgagg agctcgccgc gcgccacggc gtgacggtgt
     1681 cgtatccggc gctgaccgcg ttctgccgta agcacgggct cggccatgcg ccgccgttgc
     1741 ccaagggcag ctacgacgac gtgctcacgc cggggctcga gatgcagcac gacacgtcgc
     1801 cgcacgacgt gtccatcggc ggcaagctgc ttcgcgtaaa caccgcgtcg ctcgtgctcg
     1861 cgtactcgcg ccggctgttc ttccagttct cgccgcggtt cacgcggttc gactgcaagg
     1921 tgttcctcga cgatgccgtc gcctatcacg acggcgcgtg cgagcgctgt atgatcgaca
     1981 atacccacgt cgtcgtgctc cacggcaccg gccgcgacat ggtgcctgtg cccgagatgg
     2041 aggcgttcgg tcgccggctc ggcttttcgt tcatcgcgca cgccatcggc gatgcggacc
     2101 gcaaggcgcg cgtcgagggg ccgttctcgt tcatcgaaaa caattttctc gccggccgta
     2161 agttcgccga cttcgcagac gccaatcgcc aggctcgcga gtggtgcgac aaggtcaacg
     2221 cgaagttccg gcgcagcttg tccgcgagct ccaacgagct ttacgccgtc gagcgaccaa
     2281 agatgcgccg gctgcccgcg tggcgccccg agatctatca gctccaccag cgcgtcgtcg
     2341 atctcgaggg ctacatccac gtcgacggcc acatctactc cgtgccgtac cagctgatcg
     2401 gcaagccggt cgaggtccgc gagtccaagg acaagatccg cgtgttccgc ggtccgcgcg
     2461 aggtcgcggt gcacgacaag gtcatctcga cgctccgaaa ccagcgtcgc acgagcgccg
     2521 ctcaccgccc accgcgcggc cagcgcgctg cgcccccacc ggcactcccc gaagagcgcg
     2581 agctcgccga tgctggcgcg ccgttcgcgc actacgcggc cgcgatcaag aagcggggcg
     2641 gctcgcgttg gcccgccgcg ctgcgccggc tcgcgcagat gcgccgcgac tatccggttg
     2701 cccagctcga ggccgcggtt cgttcggcca tgcactacgg gctctacgat ctcgaccggc
     2761 tcgagcggat gatcttgaag caagtcgcca ccgacttctt cgtgttgccc gccaaccgcg
     2821 acgaagtgat cgatgaagtc gacggctctt ccgacgacct cgaggacggc gatgaaggat
     2881 gacctcgacc agctcctgcg cagccttcat ctgcgcaaga ttgccgagat cttcgacgag
     2941 gaggtcaagc gcgccgagaa ggccgacacg agttaccagg aattcgtcgc ccgcttgatg
     3001 cgcgcgcagt ggcaggccaa acaggaagcg gccctcgcct ggcgcatcga ccgcgctggc
     3061 ctccccgagg agtggactct cgagtcgttc ccgttcaagc gtcagcctgg cgttaacgcc
     3121 cgccagatcc gcaccttcgc cgagctcgag ttcgtgtcca aggcggagaa catcgtcttc
     3181 atcggcccca ccggcgttgg caagaccggc ctcgcgtccg cgctcctgct caaggcgctg
     3241 cagaacggcc atcgcggcgt gttcatgcgc gcacaggacc tgttcgatga gatgtacgcg
     3301 tcgatcgccg accgctccac gcgacgcctg ctcaaccggc tcgccaaggt cgacgtcctc
     3361 gtcgttgacg agatgggcta cctcaacctc cgccccgagc agaccaacat tttcttcaag
     3421 ctcatggagg agcgctaccg ccagcgcgcc accatcatca cgaccaacct cgactatgcc
     3481 gagtggggca acttcctcgg caacaaggcc cttgtcgacg cactactcag ccggcttcgc
     3541 caccagtgcc acaccgtcac catcgacggc ccctccctgc gtgacccgac cggctgagat
     3601 ccctcgcgct ccgcaccacc aagatgccct cgtcgccacc gcgtcgaggg cgtcgcgctt
     3661 tcggcgacca cgtgcctcgt cgacgccatc atcgacaatc acgttcgcga tcaggaatca
     3721 ctcggtgggt cggttctgac gagcagacct gggtcagttc tggcgagcgt cgcgggcatc
     3781 ctcctcgcgc cgttcttgga ggaagccgtc gccgagccgc agcgtctcgt agctcgagag
     3841 tgcgcgctgc tcgatgggat gctccctcat cgcgtcgcga atctggttgc gcgtgacttc
     3901 ggcgatctgt tcggcgagtc gtcgcggtcg aaatcgcgcc tcgaggattc gctcgatggc
     3961 gcgagcgtcg aaggcgtcgt acttggttgc gtgctcgatg gcgcccacga tgtcgtcgac
     4021 cgaccacgtg acttggagct tgaggatgtg gtcgagctcg ggccctggcg ttcgcttgcg
     4081 acggcgcagc ttgttcgcga agtcctcggc tgacggaccc caggcagcga agcgcgcttg
     4141 caagagcgtc acgtcgtagc ggccgcgccg cttgcgctcg gtcagcgaga tcaggcctgc
     4201 gccgtccggc atccgctcga gctcggtgat cgcgtggacg ccccgatcga aaacctcaag
     4261 ccggtcggac ccgacacaca cgtagacgag ctggccgatg tgctcgtcag gcaccgggta
     4321 caagttcgtc tcgtagagga tgtgcgccgt cgagtcgacg tgccgttgca cgacgtcgcg
     4381 tgtgtcgtac ggcacacgcg gcagtggctg gaggtacggc cgctcttcct cgagcatctc
     4441 ggcgagcgtt cggccagtca ctggatgtgg gcgctgcatt gcgtgcgtgt cgcgccacca
     4501 tgcgagcccc tcgcgaaact cctcgcgact gcgaaacgtc cgcccgttgc agaaattctc
     4561 gtcgacgtac cgaaatggtc gctcgacctt gcccttgtac tttggataac ggcgcggtga
     4621 gacgtgcacc gcgaagcggt agtacgccgc gaaatcgacg aagcgaatgt tcaagatcgg
     4681 ctggttgcac tcccacccgt cgacgacgcc ggccatcgta tcggtgacgc tctcgtgcgg
     4741 caccccgccg agctgcgtga agctatcgtg cagccgccgc aagatcgtcg tttgccgctg
     4801 gttgtcctcg gacgcaaacg atcgtccacg cgaccacgcc agcgtatagc tccacacgtt
     4861 ctcgatctcg ccgttctcga gtttgaaggg cgaccagtcg aattggccct gatggccggg
     4921 cgcagtctcg acgcgcaggt cggcacgctt gggcgtcgcg tgctgtgcgc gccacgcctt
     4981 gaggtgctcg cgcacaatcg tgtaagcgcc ggtgaagccc tcgccggcga gcttctcgtg
     5041 cagccgtgta gctgtcagat ccgggtactg ctcgagccac gcggtgatct tgtcgtcgaa
     5101 agcgtcgagc ttggatccgc gtggcgctcg ccgcgagggc agctcgcgat ccagcgcgct
     5161 ttcgccttgt tcgcgccgca cgtcctcgcg gaggaggatc ttcttcacag ttcgcggcgc
     5221 gatccgcagc gctcgggcga tgccacgctt ggactcgcca cgtgcggcgc gccggaccac
     5281 ttcgtagaca agctgccgtc gggcttcgtt catgatccgc tgctcgtcgc gagttgctcc
     5341 agcagcgcgg tcgtctcgcg gctcgcgagc agcgtgcgcg cgacgagcgg cccgaggatc
     5401 cgcgcatcgt ctccaacgag cccgaccggt gcgtggcgcg ttgctatccg cacgagccgc
     5461 tctgcggcac cgccgaccgc gagtaggctc ttgcgcacgt cgtttccgcc cttgcccagg
     5521 cgcggatcct cgacgctcgt cgccggaagc tcgatcgcgt gcaggtaccg cagtggatcg
     5581 gcgagcacct cgtcgcgagc cgtgggatct gccgtgcgca aaagctccgt cacgatccgg
     5641 tgcgcctgct tgctcgtgag ctcgtgcgcg cggatcgtcg ccgccatcgg gacctggttg
     5701 ccacgcggca accgcgcgag ctcgcgcgcc accgagggcg ccaggaggcc gaggcggatg
     5761 tcgtcttgca gtgcgttttc caatcgctcc gcgagcatca gccgccggct cacccacgac
     5821 ttgtcgcgcc gcagcgcctt gccgacttcg acctgtgtca gtccgtggtc tcggcacagc
     5881 gaccgcacta tccacgcctc ctcgaggtca ctcagtccat catgtccggc gttcgcagcc
     5941 accatcgcga ccttggcgcc ggctgcgtcg agcgccgtgc ggcggatcca catcactgag
     6001 tcaccgcgat ccgtcgcgat ccgtacgcgc ttgaaaccgt cgacgagcac gatccgtgtc
     6061 gcctccaccg ccgttgccac caacacggga ctcagaatcc ccatccgctc gaccgatgcc
     6121 cgcactcgcc cgagctcgtc gggtgcagcc aggcgcaagc tcgcgtaccg ctgatccagc
     6181 tcaccgatcg cgacttcgtc tcgtgactcc ttcttgtcgg tcatgggttt gggtcgacga
     6241 caccaaccct tccgcatcgt gcgcatcttg ttagtgcttc gcagtcgggc ggtgtcgtcg
     6301 gctccaccct cgccgcgttc gccgaaatcc gagccaactg atgcgtcact ttctttcgcg
     6361 ccaggcgata tcgcagctga cgcatcgcat gcagccaccg gccgcgtcga ctccgctggt
     6421 aacgctgacc tgctatccgc tggcgatgtc ggcgtgctcg tcgcgcgcac gctctgccgc
     6481 agtaccgaac tcgcggcacg tcgcacgtcg tgcagagcgc gagagcgcga ccgcactcgg
     6541 catcccgaca aacgacgatg tgcaatgtga acacggtccg cgtcgacgcg aggctcgaca
     6601 tccgagcgga cctgtgagac ccagaagggg tcgcaccgcg acttcacggt ccggcggggc
     6661 gatagagggc acggagggtc aagctccggg tcctcgctat gtgcgtcggt cgcgagcgta
     6721 gcccacgacc tcgcctgtcc gcgacgcgct gacatgtctc gccgcgaccg ccgcggtgat
     6781 gttcttcgcc gcgatgccta ttcattcggt cgcggcgact ggtgctcttc gccgcgattg
     6841 gtggtgacca cctacgccgc gatcaacact cgtagcgctc tgaccctgat catcgggtct
     6901 gctgccacgt ggctgccacg cgcatcgcat cacgatcacg cgcttgtccg gcgccctgat
     6961 gctcggctgg tcgagccgcg aggtaacgcg gctgcggcgt gtcggcgtgc ccgagagtcg
     7021 gcacgcagtt ctggttcgac gtccgagacg cggacctcgt tgtccgcgta ccggacagat
     7081 attgcaaatg agcgatgtga gccatcgcgc gtgcgatcgc aacaacggat cgccgacgag
     7141 cgacttctcg acgagcggcc tctcgacgcg gctcctcgac gagcggcctc tcgacgcggc
     7201 ttctcgacga gcggctcctc gacgagcggc ttctcgacgc ggcttctcga cgagcggctt
     7261 catcacgacg agcaccatca tcacgacgag caccatcatc acgacgagca ccaacagcct
     7321 acgcggcgag atccatccga ggtctcagat gacgagctgc tcgctgcgct gcacgccacc
     7381 cagtggcacg caaccggcgc tgcggcgcgg tgtctcgcgg tcgacgctcc tacaacctga
     7441 tcgaccgttc gaagcgcctc cgcaaggcga aggacatacc cgaccttcgt cgcgcctacg
     7501 acgcgtgtgg tggcgacctc gacatcgcgg cggcagagct cgaggtctcg cggcgctcgc
     7561 tcgagcagcg gatcgccgag ctcggctggg acgcgggctg aagtcgtctt gcctgccgga
     7621 gccatgagca gaggtcgcgc tctttccaac gtcgtgcctt cgcgtaccta ccgacatcca
     7681 gtcgctgtgt ccgaacgact aacgattact cccacagata cgtgttcgag cacttccgcg
     7741 ggatcggtcc gtatgggctg tccgtggtcg aagcggcgac gtccgatgcg cccacgtgcg
     7801 cgttgccgcc gttgtcgaag atgcagtacg tcccggtacc gtctacgaga ccatcgcgca
     7861 gaaacgtcac cgtaccgacg aggtcgtcac cgctcgagcc atccgattct ttgacgtcgc
     7921 acgtgacgcg ggcttcgttc gcgccgagcg tcgcgacgta gcggttgagc gtgtaccagt
     7981 ggatcgtgtc gttcgcctga tcgacccatt ccctcgcgaa ggatcgctgg tacacaccat
     8041 cgacggagta gctcgagcac ttgatgtaca tctcgggatc gccggcgaac atcgactcca
     8101 ttgcgtcggt gaacagcaga gcccgcaggt agaacggtga ggacgtgaca ctgggggagg
     8161 acgtcgtgtt gtcgacggag acgtcgtcgt cgatacctga acgctcgttc caaccgatcg
     8221 cgagcactgg gccggtcgga gcgtggtcag ccggtagcgt cgagctcgtg ccgtcggcgg
     8281 cgatgtacgt gaggggcgcc gtgtcatcga gttggacgaa cgtcacgagc ggtgcggtcc
     8341 gtgcccaatc gtcgacgccg agcggagcag tcagctgaag atcgggtacc acctcgagca
     8401 cgccggtagc gtcgagtacg tcgtggaggg agcggccgtc ggcgatgcga atcgctcgtg
     8461 tctcgtcgat cgaaatgaca ccgttgccct cagcgcgctc ggcaagctga gtctgaaggt
     8521 gggcccgaa
//
LOCUS       DATLBZ010000605         2520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10120307, whole genome shotgun sequence.
ACCESSION   DATLBZ010000605 DATLBZ010000000
VERSION     DATLBZ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10120307"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="VMZ53_29665"
     CDS             complement(<1..1542)
                     /locus_tag="VMZ53_29665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS32719.1"
                     /translation="MQTTTTTSIDAPLVEAFLLIEQPRQRLGHWIRALTPLDAENAPP
                     IKEVVGYYDPTCDQALINLHYDPRDLENGKLDFVVSVALLAEVAMVQPAELSEGERVR
                     FLAERLPRCTIRVVGQPNPIAVLTEIVRKIKAGRNPAVPPPIPPARSVQPRGDTSDPV
                     MLVTAKGTRDDLEQLARDSIQSATRPPTPRPPSPSPEAINPMTSSRHVIARRDRSPTA
                     EMQPGLPQKLMAQRDAVDDEDRSTQEVDPSRYRQLVDEAVDDEPLPLPRPPRAGSPDE
                     ASSEPFLPTNVKAGMIYARYLRSGRWVPVRVGALSLKGAALLTGALPRLKDRVDIALS
                     FGQHRALVRGTVGKVSTVREAAMTGAATFSVAFEHDEAAKRQLTALLTAARDAKVTIK
                     PPPPRATRRFPVEWPIALGTTRGAVKGTALDVSTGGMFVKPTVSLELGAMVGFSVVLD
                     DGSAPIAGRAKVVRLIGEREAKLCGIAAGFGLAVIEMSEADRMRWLGFLARIERRAEK
                     RVLIGA"
     gene            1762..>2520
                     /locus_tag="VMZ53_29670"
     CDS             1762..>2520
                     /locus_tag="VMZ53_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HUS32720.1"
                     /translation="MKSDDRGGYRLPLLPLRDIIVFPHMVVPLFVGRDKSLSALEEAM
                     AGDKQILLAAQRRAKTDDPNEEDIFAIGTVGHIIQLLRFPKGPVKVLVEGRARARIRG
                     FSQSDPFFVCDVDDVEEPEEDAAEVAALMRSVQDVFEDYVKLNTRIPPEMLVSVRTIE
                     EPGRLSDTIVAHVALKLKDKQELLETPSPSKRLERLGELMQGEIEIRQVEKKLRTRVK
                     KQMEKQQKEYYLNEQMQAIQKELGDRDEFKNELNE"
BASE COUNT          474 a          822 c          795 g          429 t
ORIGIN      
        1 agcgccgatg agaacgcgct tctccgcacg gcgttcgatt cgcgcaagga agccgagcca
       61 ccgcatgcga tccgcttcgc tcatctcgat caccgcgagc ccgaatccgg cggcgatccc
      121 acacagcttt gcttcgcgct cgccgatcag gcgcacgact ttcgcgcgac ccgcgatcgg
      181 cgccgagccg tcgtcgagca cgacggagaa tccgaccatc gcaccgagct cgagagacac
      241 cgtcggcttc acgaacatac cgccggtcga gacatcgagc gccgtgccct tcaccgcacc
      301 gcgagtcgtg ccgagcgcga taggccactc gacgggaaac ctgcgcgtcg cacgcggcgg
      361 cgggggcttg atcgtgacct tcgcgtcgcg cgctgcagtc agcagtgcag tgagctgccg
      421 cttcgccgcc tcgtcgtgct cgaacgcgac ggagaacgtg gccgcgccgg tcatcgcagc
      481 ttcgcgcacg gtcgagactt tgccgacggt gccacgcacg agcgcgcggt gctgcccgaa
      541 cgacagcgcg atatcgacgc gatctttgag ccgaggcaac gcgcccgtca gcagcgcagc
      601 acctttgagg ctgagcgcgc cgacgcgcac cggcacccag cgaccacttc gcagatagcg
      661 cgcgtagatc atccccgcct tcacgttcgt cggaagaaaa ggctccgacg atgcttcgtc
      721 cggtgatcca gcacgcggcg ggcgcggcag cggcaacggc tcgtcatcca cggcttcatc
      781 gacgagctgg cgatagcgcg acggatcgac ttcttgcgtc gagcgatctt cgtcgtcgac
      841 cgcatcgcgc tgcgccatca gcttctgggg tagtcccggc tgcatctccg ccgtcggcga
      901 tcgatcgcgg cgcgcgatca catgacgcga cgacgtcatc ggattgatcg cttcaggcga
      961 tggcgacggc ggtcgcggcg tcggtggacg cgtcgcggac tggatcgagt cgcgtgcgag
     1021 ctgctctaga tcgtcgcgcg tgcccttcgc ggtcacgagc atcacggggt cgctcgtatc
     1081 gccacgcggc tgcaccgagc gagcaggtgg aatcggcggc ggcaccgctg gattgcgacc
     1141 tgctttgatc ttgcggacga tctcggtcag caccgcgatc ggatttggct gaccgacgac
     1201 gcgaatcgtg cagcgcggga gccgctcggc gagaaaccgc acgcgctcgc cttccgagag
     1261 ctccgccggc tgcaccatcg caacttccgc gagcagcgcg acgctgacca caaaatcgag
     1321 cttgccgttc tcgaggtcgc gcgggtcgta gtggaggttg atcagcgctt gatcgcaggt
     1381 cggatcgtag tagccgacga cctctttgat cggtggagcg ttctcggcat cgagcggcgt
     1441 cagcgcgcgg atccaatgac cgaggcgctg gcgcggttgt tcgatgagta gaaacgcctc
     1501 cacgagtggc gcgtcgatcg aggtcgtggt cgtggtctgc ataagtccca acgcgcgcgt
     1561 actgcagcag cgcggatgcc acgaaccggt tttgcgaaat cccttaccag tggatccgga
     1621 tcttgcgagg atccttacct tgaccgtgcg actacgggac actccacccg tggcatcgat
     1681 gccacggtga gtgtggctga tttaccccga tttggcgcga attcttgccc aagtactcgt
     1741 gccccctaaa ctatcgacaa catgaaatcc gacgatcggg gtggctatcg tctgcccctg
     1801 ctcccgttgc gcgacatcat cgtgtttcct cacatggtcg tgccgctgtt cgtcggacgc
     1861 gacaagtcgc tctccgctct cgaagaagcg atggccggcg acaagcagat cctgctcgca
     1921 gcgcagcggc gcgcgaagac ggacgatcca aatgaagagg acatcttcgc gatcggaacc
     1981 gtcggtcaca tcatccagct cctgcgcttc ccgaagggcc cggtcaaggt gctcgtcgaa
     2041 ggtcgcgcgc gcgcacgcat tcgcgggttc tcgcagagcg atccattttt cgtctgcgac
     2101 gtcgatgatg tcgaagagcc cgaagaggac gccgccgaag tcgccgcgct gatgcgctcc
     2161 gtccaagacg tgttcgaaga ctacgtgaag ctcaacacgc gcatcccgcc cgagatgctc
     2221 gtcagtgttc gcacgatcga agagcccggc cggctctccg acacgatcgt cgcgcacgtc
     2281 gcactcaagc tcaaagacaa gcaagagctc ctcgagactc catcaccgtc caagcgcctc
     2341 gagcgcctcg gcgagctgat gcaaggagag atcgagattc gtcaggtaga gaagaagctg
     2401 cgcactcgcg tcaagaagca gatggagaag cagcagaagg agtactacct gaacgagcag
     2461 atgcaggcca tccagaagga gctgggtgac cgcgacgagt tcaagaacga gctcaacgag
//
LOCUS       DATLBZ010000606        22187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3490752, whole genome shotgun sequence.
ACCESSION   DATLBZ010000606 DATLBZ010000000
VERSION     DATLBZ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22187
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3490752"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..574
                     /locus_tag="VMZ53_29675"
     CDS             8..574
                     /locus_tag="VMZ53_29675"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HUS32721.1"
                     /translation="MPALEAASPVPVIGAVEPGAASALAATRDGHIGVIGTLGTVRSG
                     AYEAALRRRRPDASVTALACPLLVPLAEEGWTDDEIADAVARRYLTQLLARDPDLDTL
                     VLGCTHYPLLAGVLGRAATELAGHEVAIVDSARAMAEAARRALDADANRRAEPGRLAC
                     FATDTSRLDELAPRFLGEPITGFELVDL"
     gene            complement(777..1346)
                     /locus_tag="VMZ53_29680"
     CDS             complement(777..1346)
                     /locus_tag="VMZ53_29680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32722.1"
                     /translation="MRSWLVSLPHDLKIAFDAMDDENLPRTVREIATGVVIYVVSPKD
                     SMSDRNDLVVSYADDAVLLRLALHKALDKGEDEQAFRDRFPELFDNLEDDLKLCKSVM
                     GDLMTWLESKVSTLKTMPYKGKKVPAYLDNEENREQLFDDGVVFRTDYPVDEKTIGDK
                     LKKAATIIEVMKRRKADEARAKGLHVANG"
     gene            1536..3092
                     /locus_tag="VMZ53_29685"
     CDS             1536..3092
                     /locus_tag="VMZ53_29685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32723.1"
                     /translation="MAAKRSPILGYNHNVRYRGVVFHVQTEDSGLMNPHLFTHLFHEG
                     VIVSTRKLVYDAGSNEESIKSLMQAQHKAVMKDLRRGAFDEKIDQYLAGTPGLEPRSG
                     ADPAQPMPRGSRASTEQSPPPDEVIAEAKAAMSIAGESPAGSMSEGSPIEIVDAGLSE
                     PIELPVKGSTPPLPEDDAPTLMGSDAADVAAAVSAANTATAPTQSRTKTAERTSKSQV
                     RQTPAAGTRTPPPPGAQNVRDSYPAIEISEDDNSGRIRGPRDTAVQPTEEFADGIPAQ
                     APARPRPASHGAAALPPMKSPSRPSIQPPVVQTRAGAQSDEDLSAPVEIYNPPPPSVE
                     PPPGERSERPGQYSVSRKDGAAPIREKTGRIAAVNPNAIPSGLGRPQKGSGAVPIVPQ
                     RESSAQVPSRPQSPSSQPRVPTPAQPPPSNRVPTPQANARVHVTAPIANRPVSTPGTP
                     NPSSGVVMTRPAVIVGSPAKPPPTPPATTQRVRKAREDEGRGFGQGLISEKSLDEVIL
                     AYLSEDAEEK"
     gene            3148..3438
                     /locus_tag="VMZ53_29690"
     CDS             3148..3438
                     /locus_tag="VMZ53_29690"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HUS32724.1"
                     /translation="MPAHVKIYTREWCGYCTAALRFLEQKGVKFEHIDASGNPELRRW
                     LVEATGRTTVPQIFIDGKAIGGYTDIRDLDDRGVLDRMLAGDEAKNENRKTG"
     gene            complement(3435..4397)
                     /locus_tag="VMZ53_29695"
     CDS             complement(3435..4397)
                     /locus_tag="VMZ53_29695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32725.1"
                     /translation="MADEKAETALARPMIASELDKAGKGQLVYVGRDGEVKNPARVRT
                     QQMLQYMAFGGISAAGVALAATSFPVLVPFYLALGGRFLGTVRAVKRVNEASVALSKG
                     DAATGRALAEPVTRAWWAPGRVRALAELRVAIADALDGNSEKALDRVRRARGKLSPRL
                     IQHQFSYYTEVNLLTSLGRVKEARNLLESRGPVPTGEVLKLSHWIAQMHLWVAEAAPQ
                     PIDPKKPFLPAKEKRPLEIDDAELHDRMRKGLSMTAGSDLLMLCAWCYAYKAELDDAK
                     FCYRQAKDREGSNRLDVAMPKLAIWIDEFRKEHPDIDVPDVDEP"
     gene            complement(4401..5888)
                     /gene="cysS"
                     /locus_tag="VMZ53_29700"
     CDS             complement(4401..5888)
                     /gene="cysS"
                     /locus_tag="VMZ53_29700"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HUS32726.1"
                     /translation="MSHPFRLYDSMRRQKVSFEPLAAGKVGMYVCGPTTYAPAHIGHA
                     YSAIAFDTVRRSLEFLGWDVTYVRNVTDIDDKIINRANEAKEDPFAMSARFAADYNRD
                     MARFGVRPPTIEPKVTSHIQEIIAIVGRLIANDKAYAVDGDVYYEVEKFPPYGKLSGQ
                     TLDQLKDGARIDVDTRKRNPADFALWKSAKPGEPSWDSPWGKGRPGWHIECSAMTVTH
                     LGPTFDLHAGGKDLIFPHHENEIAQSQGADGEGTFARYWLHNGFLNFEGVKMSKSLGN
                     VFNCDQIAAAVGGEALRYFCVKHHYRSPVDFDVQATDTGVRFFSLEAADRELAKFYET
                     LKKIDDFIAEGDGGAGAVQPEAEKLVSEAREALADDFNAPVVVASLHAALTHANKLLE
                     SGKGIDKELRRRTLARLGKDMRTVGAAIGVFTQDPTAYLTERRARLVQIKAIDIGAVE
                     AKIAERAAARAAKDFKRGDEIRDELKQMGVALHDGKSGTDWSVQD"
     gene            complement(5885..9304)
                     /locus_tag="VMZ53_29705"
     CDS             complement(5885..9304)
                     /locus_tag="VMZ53_29705"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32727.1"
                     /translation="MADGEKPGAIVAPPTMPLGAAATGSPPAMPDEAETPLATMQLSP
                     QAAPLLATMMPNTAAPMPHVAPPSMPQDGRYSVVAEIARGGMGRVVEAEDTVLGRTVA
                     VKEVLSLDPETLRRFQRETKITARLEHPSIVPVHDAGIAENGVPFYVMRKVGGKPLED
                     LVARADTLNKRLALVPHIVAAANAIAHAHSRGIVHRDIKPSNILAGDLGETIVIDWGL
                     AKGMEEPDDEHVAQRVIENDDALKTRAGIVFGTPGFMAPEQLRGKPVNEQCDVYALGA
                     TLYHLLARRPPHHDKSADKMMHAAVAGPPVPLTEIVEGVPPELVAIVDKALAFDAANR
                     YPDARALAQDLQAFLTGQLVAAHFYTPREKFFRFLRRNRAVVGVVLASLLTLAVVATI
                     LFVRIRDERDIANEQRAIADTQRARADKQRDEVLQKSRQLILANARHLSTTDPTRAAA
                     LVRPLLDSELWREARDVIAAARSNGIAFALDASRRTLSLELSRDGQRALAAGDDGLIR
                     IYDLAKRESRVVIDMKGAMMARFGDGERKIVLYEGNRLAIVDPGTAARRDVTTPTPIA
                     RLEVAGPIAYWTDAAGVVFKLDLAAGGPERVTVTEPIKIASPSPDGRWIALGGAKHLW
                     LIDRTSPNAPPQNITEGDVKAISWSGDSEHLVMLIDELAVNIEMDPVPTIFRRITVGD
                     RFAVAYSAGRIFSAGPTGIAIVNKDMPRDPREQAVRSPGPDHTLGVYEARDRVVVSAK
                     PQGVIVVLSDNGDHVLTAPAPIAMVATSARGQWIVAAAEERLLVWDLDAIEPRAISVF
                     PPSSARFTNGDQLIATYEGRGAEWIDLRAHKSLPLDDDMEGLIGAATSPDGALAVVVD
                     LTRKAWLVAGVAQPQPIEGEVSAAAFVDNTRLVIGGTGGLRLEDQESHSKLVLFAHEK
                     AARAIETSAADGGWVAATFEDGLVWRKHLSDGATTQITVDSQSPIRIAIAGDGTVLLG
                     IGGELRAWRTDGKLDVVLGGVKTIKHVSFIAPTTALAVTDEAMAYQVDTRTRTTLSSQ
                     TIGHAASHADTGGLVAGLSALGGVEVYDPLSHWSWPLVTPQKGQPPFSFVHISPDSRR
                     VLAMTSQSLLVWTLELPSTAEATVQWLDRLTNATAEGPSEPLGWRATNSPP"
     gene            complement(9345..10451)
                     /locus_tag="VMZ53_29710"
     CDS             complement(9345..10451)
                     /locus_tag="VMZ53_29710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32728.1"
                     /translation="MAEEKDPGAPVPKDAIDPELIKLARKRTKFGIITCAGIIFLALY
                     FIVKLSSDRRFSGDDESPATATPADIAAGKVEDEQLVMVQGEVVMAHAIRVGAEKKSL
                     GLRVAPLRGTNEKVWVVLDGDGYAKPTMGSFTGRLRELDDLPFASTVRDYAKAHPQAV
                     FATASATRAGFAANKITTVSGEQVEVRDSDRVGFEIVDPTAATVVCTFNEKHETVDAC
                     SKELRDAGIPTTATPRTGREQAYFDVTNMENAVAAMTTKLEAAKLWGTRVDPVTRHFE
                     TTWGKLKTASPAGFTVETTTIPDAQIDLIGLYVVRGIPADAYAVILGEHPQDYWYVMP
                     VTVVLAIIALLFAWALVRAVKRDLLGPKPESPDA"
     gene            10450..11898
                     /gene="xseA"
                     /locus_tag="VMZ53_29715"
     CDS             10450..11898
                     /gene="xseA"
                     /locus_tag="VMZ53_29715"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HUS32729.1"
                     /translation="MAVREPAKSYCLLGHRATQVGCDEEWPWLPLDVPSEASTREKPW
                     SVSDVVRGAARTLDAIGLVWLEGEVTQVTKPASGHLYFSLRDRESVLPAIMWGRDSQR
                     IKFRIEPGQRLRVRGRLGVYERDGRMQLYADFAEPAGLGAEAIALQQLKDKLAAEGMF
                     SPQKKRPLPRFPKRIGVVTSKHGAAVHDIIRTIHRRFPTPILIADAAVQGETAPHQIV
                     QGMAMVVRAGVDVVIVGRGGGAVTDLTAFNHERVVRTISRCPVPVISAVGHEVDLSLA
                     DLAADARASTPTAAAELVVVDGHAVADVLRKELRRLDREMHHVLARAHQDLDRRTMAL
                     HARGERALGATRHQLQQLQHRLAGLHPRAQILRRRTVLGDLERRLASLHPGARIARSR
                     HDLASLIARRDTAMRELLEERRTDLAQLGAQMAALSPLAVLDRGYAMVRAGDVIVRDA
                     AQVNAKDRLHVTLAQGSIDVIVEKIQKGRDSK"
     gene            11895..14129
                     /locus_tag="VMZ53_29720"
     CDS             11895..14129
                     /locus_tag="VMZ53_29720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUS32730.1"
                     /translation="MSRAKSKRAGTRGGGGTNVGKKPTRAHLPDDLADAAEAALDDLL
                     SSSHTSLDDDSDEPATRAREGGKGRGLSTHEVSLEEIIPIDVDMDDEGDDDPLTRARP
                     RRLPSKVAPLASATPKAPAPTQTSSLDVNIDTTNPVEPSDVEELDDDEPATAFQPKAS
                     PPSRNSSTHTIAREPSVAKPLPSPPARISSPHAVATPARTAHTIPRTGQTPPHGGRVS
                     APLAPEDTSAHTIARDDASFGAGSSNGGSPTSAARTPTPQPSRAPLPPPSGSSMPSSS
                     RLPLPPPTESRLHGGRVDPETIEKTTGRETKAGEADARKAWLQSARFDRSSGDHEPAP
                     LDEVDLDSEPFERPDEDRASLDHIDLDSAPFERSDSASPGRVPDSAPFGRVPDSASFT
                     RVPDSAPFERSGRSARFEVGPLDDALAGLEAEPRGASQSGEERALFEMPALRFAVYED
                     PGYLSSAQNAIVEAGHVVAAAGAGRDALKRVGDLIRTGSIDVVLVSLPGGETIIDAAM
                     SLEPRRPTIIASLSASSVEAVSAATLSGADLATTRPHDVERIAPLALAAARIDAERRV
                     AASAKGAEQVLRQRLEDLADAEPGGLLPFEHFQRVLEIEIKRAKRFIYPLSVALFAVE
                     VTPPPPPAGILGILRARAGNALINTIRDIDIATQLDHERFLVLLPYTDLKGAAAVGKR
                     VIAAVAKGHPVVSDGRTFPPRVIGACAGMKKGEPISFAKLMKDATRALESARRDGADL
                     AVQP"
     gene            14126..14956
                     /locus_tag="VMZ53_29725"
     CDS             14126..14956
                     /locus_tag="VMZ53_29725"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HUS32731.1"
                     /translation="MSKPAVAISGKTRVAAVLGWPVEHSRSPAMLNAAFAVDQIDAVL
                     IPIGVPPDGLKSVVAALRAMNVLGASVTLPHKVAVAGLCDELSPEARAIGAVNCLQFT
                     EGRLVGHNTDCDGFVDGLVAAGFELRGKRAIVLGAGGAARAVAHGLRGGRALEVVARR
                     PAEVVWAKAWPWTDEQLRDCFSRADLVVDCTSASLGDDDAVFADALPLDALPAHAWVT
                     SLIYNRKPKLLERASERGHSTLDGKPMLVHQGARAFHIWTGLPAPVDVMARALEASLA
                     "
     gene            15002..15727
                     /locus_tag="VMZ53_29730"
     CDS             15002..15727
                     /locus_tag="VMZ53_29730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32732.1"
                     /translation="MRNFRRFRPAPFVVLLLLFTGGGVAWGERHTADLYIEDPYAPHH
                     TTGSEARLGTAVGFLYNEPVDVTALGASAAIGYRWGRFTVESEYTYLGFQVRGPDNTR
                     LGAGQRLAFLGRFDVIRLGPRIVGQNSLLSIYIEGGAGTSWNRWWRPAYDEASRIVPN
                     DTRRAEGQVGFGIAIDHRLQEPIGFPRRVGWFLGWRVAMSPHQPMAASVCRGVTCSPI
                     PMMDEDRLVDRSMLFQSSLAFTF"
     gene            complement(15728..16051)
                     /locus_tag="VMZ53_29735"
     CDS             complement(15728..16051)
                     /locus_tag="VMZ53_29735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32733.1"
                     /translation="MRILSLFAVTVLAFACGGGKPKGQSSMVNEGDTSPTCCCKTIPT
                     TAEKEIVPVYAQANRMECSSNRGDCVDDVQCNGQEGGDTTQQQPSNDGVPPPPVLEPS
                     TGTIQ"
     gene            complement(16073..16651)
                     /locus_tag="VMZ53_29740"
     CDS             complement(16073..16651)
                     /locus_tag="VMZ53_29740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32734.1"
                     /translation="MLLDTTARWLLVLHTALGVTAVGASTHLVLWTRRYLRGQYGKRR
                     AVRRFAWIVLAFQLGAFVAGNIMYPTYKVEVRAAYLENQVAISGEAATHERELGKITA
                     REGGQAYESPATREMVRRAAEAARWFDIKEHWVALGLLASAALLLILWRWNPQEDGLE
                     IAPVVSALAWMAAGTIWFGAIVGVLTASWRAV"
     gene            complement(16734..18167)
                     /gene="ftsZ"
                     /locus_tag="VMZ53_29745"
     CDS             complement(16734..18167)
                     /gene="ftsZ"
                     /locus_tag="VMZ53_29745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HUS32735.1"
                     /translation="MALIEFDDVSHAKILVIGVGGGGGNAVNTMISGNLDGVEFVVAN
                     TDMQALEANMAPHKIQLGNALTKGLGAGANPEVGRRSAEESMQQIADLISGADMVFVT
                     AGMGGGTGTGAAPVIAQIARDCGALTVGVVTKPFGFEGKKRARQAVEGIDRLGAAVDT
                     LIVIPNNRLLALVGQNTSMVEAFRKADSVLLNAVQGISDLMTVPGLINVDFADVRTIM
                     AGMGRALMGTGIGQGKKRAGEAAEMAISSPLLEDVSIEGATGILINITGGPDLTLHEV
                     NEASSLIQQAAHEDANIIFGSVIDPNLSDEVRITVIATGFDRAAATLPVEPQVQTRMQ
                     AASKPTYKATASQVSLPYDVSTQITKEYPPPMPPRASQPKMQAHVVDEPDVHIEWASP
                     DVEVSPMHDSYRSLEDLNVPEGEPSQRMAAGSGPATPVDGNPLVQPDRKRAQSRPSMK
                     AVLAGEIDTESELDVPTFIRRHNTTHG"
     gene            18261..19373
                     /locus_tag="VMZ53_29750"
     CDS             18261..19373
                     /locus_tag="VMZ53_29750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32736.1"
                     /translation="MIVLFAVACGDDASSSSDAGGDGRNLSGDGNGQFGNGPTLGGCP
                     VLPDNHIFNTKIDQLPVDPNSAAYIATIGGTTRLHLDLGQQTDQSQADFYGIPYNVAA
                     MSAATWPTVAYTSPDPGMTWDPKPESDCADAAHSIVSPCDSIAQPKIPIMTTPLVEGG
                     INTASSHMPYGDHHILILDTDSCLLWETYHSYFVSSAWQIFGSAVWDMRSNNLRPDSW
                     SSADAAGFPILPLLLKEPEATSGTIKHALRFTIDTPKIRGVYTWPATHEASNVTGAGN
                     PPYGQLFRLKASYQIPANYNVQSKAILTAMKEYGMYIADGGSDMYVTGAPSANWMDST
                     FSEVQSVAASNFEAVSLAPIKARAGWSATSAAVPAP"
     gene            complement(19377..20606)
                     /gene="ftsA"
                     /locus_tag="VMZ53_29755"
     CDS             complement(19377..20606)
                     /gene="ftsA"
                     /locus_tag="VMZ53_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HUS32737.1"
                     /translation="MAKPKSNEIVVGLDIGTTKIACIAGEVTEDGVDIIGIGTAPSKG
                     LRRGYVVNIDATVTAIQQAVDEAENMAGCEISSVYAAISGAHVRGLNSHGIIAVKDKE
                     VREADIARVIEQAKAVAIPMDREVLHVLPQQYVVDDQDGIRDPLGMAGVRLEAKVHMV
                     TTAVASAQNVIKCANRCNLQVADIVLESLASAQAVLEDDEKELGVALIDIGGGTCDLM
                     IYCDGAIVHTSVLPLGGGHITNDIATVLRTPLDSAEKIKRKYGCAWRGHVDDSETMEV
                     PSVGGRGPRVLPRMQLVEVIEPRVEEIFEHVKKELMRSGYYDSLGGGIVLTGGATAMD
                     GVCDVAENTLGLPTRRGVPTKIGGLVDVVRSPAYATGVGLVMFGADHGRSAQTPSREM
                     DRPGLFKRAWGRLAEMF"
     gene            complement(20613..21536)
                     /locus_tag="VMZ53_29760"
     CDS             complement(20613..21536)
                     /locus_tag="VMZ53_29760"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HUS32738.1"
                     /translation="MDRSLGARRDGAKAAPPSASRLTFKRRGNRRKTQPGSVWSKLPK
                     PAVIADACSRVLRRSVPALIGLAVLGTVGGTAWAGYRFVTHSPRFAITNIDVSGNRQV
                     ATDEIASTVPAHVGDNVFAVDLDEVARAAKTNPWIAQAEAHRVLPHTITVSVREHVAA
                     AVVELSGLYLADEAGHPFKKADLTLDEGAGLPIITGLDRATFNANPDGGAAQVRAALD
                     ALARWNDSSDRPSIGELHLDPHGNLTLVTYEHAIKIRLGVANDFLATRLATFDATWNE
                     LSSSERSRMRAIHLDAHSDQVTVAFAPEKDQ"
     gene            complement(21620..21910)
                     /locus_tag="VMZ53_29765"
     CDS             complement(21620..21910)
                     /locus_tag="VMZ53_29765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32739.1"
                     /translation="MSRWLGLFLVLGLTATSAEAQVFKPKTTKTTKAKPEKASSDSSE
                     KKPKAARTSPGKKRVTAKSKKSSSRSDDQASGSSSKDSDKDFVKITDDDDIE"
     gene            complement(21916..>22187)
                     /locus_tag="VMZ53_29770"
     CDS             complement(21916..>22187)
                     /locus_tag="VMZ53_29770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32740.1"
                     /translation="AKGLERVTVTYDQYSETDDDGDGDPHTQRTTVVVPRTEVVLPEL
                     EDLVRAMAHCTGTVCRSDDREFRFTFKGDQLRRLEVVERPPCVTR"
BASE COUNT         3617 a         7335 c         7427 g         3808 t
ORIGIN      
        1 gaccgcgctg ccggcgctcg aggcagcgag cccggtaccg gtgatcggcg cggtcgagcc
       61 gggggcggcg agcgcgcttg ccgcgacccg cgacggccac atcggagtca tcggcaccct
      121 cggcaccgtc cgttcggggg cctacgaggc ggcgctgcgc cggcggcgcc ccgacgcgtc
      181 ggtgaccgcg ctggcctgtc cgctgctcgt gccgctcgcc gaggagggct ggaccgacga
      241 cgagatcgcc gacgcggtcg cgcgccgcta cctcacgcag ctgctcgcgc gcgatccgga
      301 cctcgacacg ctggtcctcg gctgcacgca ctacccgctg ctcgccggcg tgctcggccg
      361 cgccgccacc gagctcgccg gtcacgaggt ggcgatcgtc gattccgcgc gcgcgatggc
      421 cgaggccgcg cgccgcgcgc tcgacgccga cgcgaaccgg cgcgccgagc cgggccggct
      481 ggcgtgcttc gccaccgaca cgtcacgcct cgacgagctc gcgccgcgat tcctcggcga
      541 gccgattacc ggcttcgagc tcgtcgatct gtgagcccgc aagcgtaagc gtaagcggtg
      601 caggccccgc ttcggggcat ttcgccagtc tcgcgagtga cgaagtggct cgccaagtca
      661 gggcagagcg cagccgaacg ccgaacgccg aacgccgaac gccgaacgcc gaacgccgaa
      721 cgccgaaggg cgggtgctcg cttacgctta cgcttacgcg tacgagggct gcgcgatcag
      781 ccgttagcga cgtgcaggcc tttggcccgc gcttcgtcgg ctttgcgacg cttcatcact
      841 tcgatgatcg tcgctgcctt cttgagcttg tcgccgatcg tcttctcgtc gacgggataa
      901 tccgtgcgga agacaacgcc gtcatcgaac agctgttcgc ggttctcctc gttgtcgagg
      961 tacgcgggga ccttcttgcc cttgtacggc atggtcttca gcgtggagac cttcgactcg
     1021 agccacgtca tcagatcgcc catcacgctc ttgcagagct tgaggtcgtc ttcgaggttg
     1081 tcgaagagct cggggaagcg atcgcgaaac gcttgctcgt cctcgccctt gtcgagcgct
     1141 ttgtgcagcg cgagacggag caggaccgca tcgtcggcgt acgagacgac aaggtcgttg
     1201 cgatcactca tcgagtcctt cggagacacg acgtagatca cgacgcctgt cgcgatttcc
     1261 cggactgtcc gggggaggtt ctcgtcatcc atcgcgtcga acgcgatctt gaggtcgtgt
     1321 ggaagagaca ccagccaaga ccgcaccaac tcgacaaact tcgactccgc aggattcgtc
     1381 acgggatatg cctaccccgc ggttgcgggg cctccaaatc agcttcgaga ttgaagcccg
     1441 agttgggtga gtaccaaccc cgcgaacgaa ttgcaatgct tgggaggcga gcctacgacc
     1501 cggcgggccc gtccgtgcta ccctcggact cctaggtggc ggccaagcgt tcacccatcc
     1561 tcgggtacaa ccacaacgtc cggtaccgag gcgtggtttt tcacgtgcag accgaggact
     1621 cggggctcat gaacccgcac ctcttcacgc acttgttcca cgagggcgtg atcgtctcga
     1681 ctcgtaagct ggtttatgac gccggctcga acgaggagtc gatcaagtcc ctgatgcagg
     1741 ctcagcacaa ggccgtgatg aaggacctcc ggaggggggc ctttgacgag aaaatcgacc
     1801 agtacctcgc cgggacgccg ggcctcgagc ctcgatccgg agcggacccg gcccagccga
     1861 tgccgcgtgg ctcgcgcgcg tcgaccgagc agtcgccgcc gccggacgaa gtgatcgcgg
     1921 aagcgaaggc ggccatgagc atcgccggtg agtcgccggc cggcagcatg agcgaaggct
     1981 cgccgatcga gatcgtcgat gccggtctga gcgagccgat cgagctgccg gtcaagggca
     2041 gcacgccgcc gttgcccgaa gacgacgcgc cgacgctcat gggcagcgac gcggctgacg
     2101 ttgcagcagc ggtcagtgcc gcgaacaccg cgacggcgcc gacgcaatcg cgaacgaaga
     2161 ccgccgagcg cacgagcaag tcgcaagttc ggcagacgcc tgctgcagga acgcgcacgc
     2221 cgccgccccc cggtgcgcag aacgttcgtg actcgtatcc cgcgatcgag atcagcgaag
     2281 acgacaacag cggacgcata cgcgggccac gcgacactgc ggtccagccc accgaagaat
     2341 tcgccgacgg aattccagcg caggcacccg cgcgtcctcg ccccgcgagc cacggtgcgg
     2401 ccgcgctgcc accgatgaag tcgccgtcgc gtccgagcat tcagccgccc gtcgtgcaga
     2461 cgcgcgccgg tgcgcagagc gacgaagatc tgagcgcgcc cgtcgagatc tacaacccgc
     2521 cgcccccgtc ggtggagcca ccgccgggtg agcgctccga aaggccgggg caatattcgg
     2581 tcagtcgcaa agatggcgcg gcgcccattc gcgagaagac tggacgcatc gccgcggtca
     2641 accccaacgc gattccgtcg gggctggggc gcccgcagaa gggctccggc gcggtgccga
     2701 tcgtgccgca gcgcgaatcg tcggcgcagg ttcccagtcg cccgcagtcg ccgtcgagcc
     2761 agccgcgcgt gccgacgccc gcgcaaccgc cgccgagcaa tcgcgtgccg accccgcaag
     2821 caaacgcgcg cgtgcacgtg acggcgccga tcgcgaatcg cccggtatcc acaccgggta
     2881 cgccgaatcc gtcgagcggc gtcgtgatga cgcgtccagc ggtgatcgtt ggctcgccag
     2941 ccaagccgcc gcccacaccg cctgccacca cgcagcgcgt gcgcaaggct cgcgaggacg
     3001 aaggccgcgg tttcggccag ggcctcatct ccgaaaagag cctggacgag gtcatcctcg
     3061 cgtatctgtc cgaagacgcc gaggagaagt aaaacctcgc acgtgaggca ggctggcggt
     3121 ggctcaccgc agagctacgt tgagatcatg ccggctcacg taaaaatcta tacgcgcgag
     3181 tggtgtggct actgcacggc agcgcttcgc ttcctcgagc aaaagggcgt gaagttcgag
     3241 catatcgacg cctcgggaaa tcccgagcta cgccgttggc tcgtcgaagc cacgggacgc
     3301 acgaccgttc cgcagatctt catcgatggc aaagcgatcg gcggctacac cgacattcgc
     3361 gatctcgatg accgcggcgt gctcgatcgg atgctcgccg gcgacgaagc gaagaacgag
     3421 aatcgcaaga ccggctaggg ctcgtcgacg tcgggtacgt cgatatcggg atgctctttg
     3481 cgaaactcgt cgatccagat cgcgagcttg ggcatcgcta cgtcgaggcg gttcgagcct
     3541 tcgcgatcct ttgcttggcg atagcagaac ttggcgtcgt cgagctcggc tttgtacgcg
     3601 tagcaccacg cgcagagcat cagtagatcg ctgccggcgg tcatcgacag acctttgcgc
     3661 atgcggtcgt gcagctcggc gtcatcgatc tcgaggggcc gcttctcttt cgcgggcaag
     3721 aatggcttct tcggatcgat tggctgcggc gctgcttcgg cgacccacaa atgcatctgc
     3781 gcgatccagt gcgagagctt gagcacctcg cccgttggga cggggcctcg cgactcgagg
     3841 agattgcgcg cttcctttac gcgaccgagc gacgtgagga ggttgacctc cgtgtaatac
     3901 gagaactggt gctggatgag gcgcggcgag agcttgccgc gcgcacggcg aactcgatcg
     3961 agcgccttct cgctgttgcc gtcgagcgca tcggcgatcg caacgcgcag ctcggcgaga
     4021 gcacgcacgc ggccgggcgc ccaccacgcg cgagtgacag gctccgcgag agcgcggccc
     4081 gttgcggcgt caccctttga tagtgcgaca ctcgcttcgt tgacgcgctt gaccgcgcgc
     4141 accgtgccga ggaagcgacc gccgagcgcg agatagaatg gaacgaggac gggaaacgac
     4201 gtggccgcga gcgcgacacc cgctgcgctg atgccgccga acgccatgta ctgcagcatt
     4261 tgctgcgtgc gaacgcgcgc gggattcttc acctcgccat cgcggccgac gtagacgagc
     4321 tgacctttgc ccgccttgtc gagctccgac gcgatcatcg gtcgggcgag cgcggtctcg
     4381 gctttctcgt ccgccatgat ctagtcttgc acggaccagt cggtgccgct cttgccgtcg
     4441 tgcagggcga cgcccatttg cttcagctcg tcgcgaatct cgtcgccgcg tttgaaatcc
     4501 ttggcggcgc gcgcagctgc acgctcggcg atcttcgcct cgacggcacc gatgtcgatc
     4561 gccttgatct ggacgagccg ggctcgccgt tcggtcaggt aggccgtcgg gtcctgcgtg
     4621 aacacgccga tcgccgcgcc gaccgtgcgc atgtccttgc cgagccgcgc gagcgtacgg
     4681 cggcgaagct ctttatcgat gcccttgccg ctctcgagca gcttgttcgc atgcgtgagt
     4741 gcggcgtgca gtgacgcgac gacgacgggc gcgttgaagt catccgcgag tgcctcgcgt
     4801 gcttcggaga caagcttctc cgcttccggt tgcacggcac cggcgcctcc atctccctcg
     4861 gcgatgaagt cgtcgatctt cttgagcgtc tcgtagaact tcgcgagctc gcgatcggcc
     4921 gcctcgagcg agaagaaccg cacgccggtg tcggtcgcct gcacgtcgaa gtcgacgggc
     4981 gaacggtagt ggtgcttgac gcagaagtag cgcagcgcct cgccgccgac ggcagctgcg
     5041 atctgatcgc agttgaagac gttgccgagc gacttgctca tcttcacgcc ttcgaagttg
     5101 agaaagccgt tgtggagcca gtagcgagcg aacgttccct caccatcggc gccttgcgac
     5161 tgcgcgatct cgttctcgtg gtgcgggaag atgagatctt tgccgccggc gtgcagatcg
     5221 aacgtggggc ccagatgcgt gaccgtcatc gccgagcact cgatgtgcca accggggcgg
     5281 cctttgcccc acggtgaatc ccacgacggc tcgccgggct tggcgctctt ccacagcgcg
     5341 aagtcggcgg gattgcgctt gcgcgtgtcg acatcgatgc gcgcgccgtc cttcagctga
     5401 tcgagcgtct ggccagagag cttgccgtac ggcgggaact tctcgacctc gtagtagacg
     5461 tcaccgtcga cggcgtacgc cttgtcgttg gcgatcaggc gcccgacgat cgcgatgatc
     5521 tcttggatgt ggctcgtgac cttcggctcg atcgtcggag ggcggacgcc gaaccgcgcc
     5581 atgtcgcggt tgtagtcagc ggcgaagcgc gccgacatcg cgaatggatc ttccttcgcc
     5641 tcgttcgcgc gattgatgat cttgtcgtcg atgtcagtga cgttgcgaac gtaggtgacg
     5701 tcccagccga gaaactcgag gctccggcgg accgtgtcga acgcgatcgc cgagtacgcg
     5761 tggccgatat gcgcgggcgc atacgtcgtc ggaccgcaca cgtacatgcc gaccttgccc
     5821 gcagcgagcg gctcgaacga cactttttga cggcgcatcg aatcgtagag gcggaacggg
     5881 tgactcacgg cgggctgttt gtagcacgcc aacccagggg ctcggacggc ccctcggcgg
     5941 tcgcattcgt gaggcggtcg agccactgca cggtcgcctc tgccgtcgac gggagctcga
     6001 gcgtccaaac gagcaaggac tgcgaggtca tggcgaggac gcgacggcta tccggagaga
     6061 tgtgcacgaa cgagaacggc ggctggcctt tctgcggagt gacgagcggc cagctccaat
     6121 gcgagagcgg atcgtagacc tcgacgccgc cgagcgcgga gagcccagcg acgaggccgc
     6181 ctgtatcagc gtgtgatgct gcgtggccga tcgtctgcga gctgagcgtc gtgcgggtcc
     6241 gcgtgtcgac ttgataggcc atcgcttcgt cggtgacggc gagcgcggtc gtcggtgcga
     6301 tgaacgagac gtgcttgatc gtcttcacgc cgccgaggac aacgtcgagc ttgccgtcgg
     6361 tgcgccacgc acgcagctcg ccaccgatac cgagcagcac cgtgccgtcg ccggcgatcg
     6421 cgatccggat cggcgactgc gaatcgacgg tgatctgcgt cgtcgcgccg tcggacaagt
     6481 gcttgcgcca gacgaggcca tcttcgaacg tagcagcgac ccagccacca tcggccgcgg
     6541 acgtctcgat cgcgcgggca gctttctcgt gtgcgaacag gacgagcttg ctgtgcgatt
     6601 cctggtcttc gaggcggagg ccgccggttc cgccgatgac gaggcgcgtg ttgtcgacga
     6661 acgcggcggc gctgacctcg ccctcgatcg gctgcggttg tgcgacgccg gcaacgagcc
     6721 acgccttgcg ggtcagatcg acgacgactg ccagcgcacc gtcgggtgac gtcgccgcac
     6781 cgatgaggcc ttccatatcg tcgtcgagcg gaagcgactt gtgcgcgcgg agatcgatcc
     6841 attccgcgcc acgtccctcg tacgtcgcga tgagctgatc gccgttcgtg aagcgggcgc
     6901 tgctcggcgg gaacacgctg atggcgcgcg gctcgatcgc gtcgaggtcc cagacgagca
     6961 gacgctcctc cgctgcggcg acgatccatt gcccgcgtgc tgatgtcgcg accatcgcga
     7021 tcggtgcagg tgccgtaagg acgtggtcac cgttatccga gaggaccacg atcacgcctt
     7081 gcggcttcgc cgagacgacg acgcggtcgc gcgcttcata gacgccgagc gtgtggtcgg
     7141 ggcccggcga acgcacggcc tgctctcgcg gatcgcgcgg catgtccttg ttgacgatcg
     7201 cgatgccggt gggaccggcg ctgaagatgc gcccggcgct gtatgcgacc gcgaagcgat
     7261 caccgacggt gatcctccgg aagattgtcg gcacgggatc catttcgatg ttcacggcga
     7321 gctcgtcgat caacatgacg agatgctcgg aatcgcccga ccacgagatc gctttgacgt
     7381 cgccttcggt gatgttctgc ggcggcgcat tcggcgaagt gcgatcgatg agccagaggt
     7441 gcttcgcgcc gccgagcgcg atccaccgtc cgtcggggga cggcgacgcg atcttgatcg
     7501 gctcggtgac ggtcacgcgc tcggggccgc ctgctgcgag atcgagcttg aagacgacac
     7561 cggcagcgtc ggtccagtac gcgatcggtc ccgcgacttc gagacgtgcg atcggcgtcg
     7621 gtgtggtgac gtcgcgtcgt gcagcggttc ccggatcgac gatcgcgagg cggttaccct
     7681 cgtagaggac gatcttgcgt tcgccgtcgc cgaagcgggc catcatcgcg cccttcatat
     7741 cgatgacgac gcgcgactct cgcttcgcta gatcgtagat gcggatcagg ccatcgtcac
     7801 cggcggcgag cgctcgctgg ccatctcgcg acagctcgag cgagagcgtg cgcctcgatg
     7861 cgtcgagcgc aaacgcgatg ccgttgctgc gcgctgcagc gatcacgtcg cgcgcttcgc
     7921 gccatagctc ggaatcgagg agcggacgca cgagagccgc ggcccgcgtc ggatccgtcg
     7981 tcgagagatg gcgcgcgttc gcgaggatga gctgccgcga tttctgcagc acctcgtcgc
     8041 gctgcttgtc cgcacgcgct cgttgtgtat cggcgatcgc gcgttgctcg ttcgcgatgt
     8101 cacgctcgtc gcgaatgcgc acgaacagga tcgtcgcgac gacggcgagc gtcagcagcg
     8161 atgcgagcac gacaccgacg accgcgcggt tgcgccgcag gaatcgaaag aacttctcgc
     8221 gcggcgtgta gaagtgcgcg gcgacgagct ggccggtcaa gaaggcctgt agatcctgcg
     8281 cgagcgcgcg cgcgtcggga tagcgattcg ccgcgtcgaa cgcgagtgcc ttgtcgacga
     8341 tcgcgacgag ctccggcggc acgccctcga cgatctccgt cagcggcacg ggcggtccgg
     8401 cgactgccgc gtgcatcatc ttgtcggcgc tcttgtcgtg atgcggcggc cgtcgcgcga
     8461 gcaagtgata gagcgtcgcg ccgagcgcgt agacgtcgca ctgttcgttg acgggcttgc
     8521 cgcgcagctg ctcgggcgcc atgaagcccg gcgtgccgaa cacgatgccc gcgcgcgtct
     8581 tgagcgcatc gtcgttctcg atcacgcgtt gcgcgacatg ctcgtcgtcg ggctcttcca
     8641 tccccttcgc tagaccccag tcgatgacga tcgtctcgcc cagatcgccg gcgaggatgt
     8701 tcgacggctt gatgtcgcga tgcacgatgc cacgcgagtg cgcatgcgcg atcgcattcg
     8761 ctgcggcgac gatatgcggc acgagcgcga gccgcttgtt gagcgtatcg gcgcgcgcga
     8821 cgagatcctc gagcggcttg ccgccgactt ttcgcatcac gtagaacggg acgccgttct
     8881 cggcgatgcc cgcgtcgtgc acgggaacga tcgatggatg ctcgaggcgc gcggtgatct
     8941 tcgtctcgcg ttgaaaccgg cgcagcgtct cgggatcgag tgacagcact tctttcaccg
     9001 cgacggtccg gccgagcacg gtgtcttcgg cctcgacgac gcgccccatg ccaccccgcg
     9061 cgatctcggc gacgacgctg tagcgtccat cctgcggcat cgacgggggc gcgacatgcg
     9121 gcatgggagc cgcagtgttc ggcatcatcg tcgcgagcag tggagccgcc tgcggggaca
     9181 gctgcatcgt cgcgagcggt gtctctgcct cgtcgggcat cgccggcggc gaccccgtcg
     9241 ctgcagcacc gagcggcatc gtcggaggcg cgacgatcgc gccgggcttc tccccgtcag
     9301 ccacgcggcg agtatgtcac gacgcgatcg gaggcggtga acggctacgc gtctggcgat
     9361 tcgggcttcg ggccgagcaa gtcgcgcttc acggcgcgga cgagcgccca cgcgaacagc
     9421 agcgcgatga tcgcgagcac gaccgtgacg ggcatgacgt accagtagtc ctgcgggtgc
     9481 tcgccgagga tgacggcgta tgcgtcggcc ggaattccgc ggaccacgta gaggccgatg
     9541 agatcgatct gcgcatcggg aatcgtcgtg gtctcgacgg tgaagcctgc ggggctcgcc
     9601 gtcttgagct tgccccacgt cgtctcgaag tggcgcgtga cgggatcgac gcgcgtcccc
     9661 cagagcttgg cggcttcgag cttcgtcgtc atcgccgcga cggcgttctc catgttcgtc
     9721 acgtcgaagt acgcctgctc gcggccggtg cgcggcgtgg ctgtcgtcgg gatgccggcg
     9781 tcgcgcagct ctttgctgca cgcatcgacg gtctcgtgct tttcgttgaa cgtgcagacg
     9841 accgtggcgg cggtcgggtc gacgatctcg aagccgacac ggtccgaatc ccggacctcg
     9901 acttgttcgc cactgaccgt cgtgatcttg ttcgctgcga agcctgcgcg cgtggcagat
     9961 gccgttgcga agaccgcttg cggatgggcc ttcgcgtagt cacgcacggt cgacgcgaat
    10021 ggcaggtcgt cgagctcgcg caggcggccg gtgaacgagc ccatcgtcgg tttcgcgtag
    10081 ccgtcgccgt cgaggacaac ccagactttt tcgttcgtgc cgcgcagcgg ggcaacgcgc
    10141 aggccgaggc tcttcttctc ggcgccgacg cgaatcgcgt gcgccatgac gacctcgcct
    10201 tgcaccatga cgagctgctc gtcctcgacc ttgccggcgg caatgtcagc aggcgttgcg
    10261 gtggcgggtg actcgtcgtc gccggagaac cggcggtccg agctcagctt cacgatgaag
    10321 tacagcgcga ggaagatgat gccggcgcac gtgatgatgc cgaacttggt gcgcttgcgc
    10381 gcgagcttga tcagctcggg atcgatcgcg tccttgggaa cgggcgctcc gggatccttt
    10441 tcttcagcca tggcggtgcg agaaccggct aaatcctact gtttgttggg acaccgcgct
    10501 acccaggtcg gttgcgacga agaatggcct tggttaccgt tggatgtgcc atcggaagcc
    10561 agcactcgcg agaagccctg gagcgtctcg gacgtggtgc ggggagccgc gcgcactctg
    10621 gacgccatcg ggctggtctg gctcgagggc gaggtcacgc aggtcacgaa gccagcgtcg
    10681 gggcacctgt acttctcgct gcgcgatcgc gagtcggtcc tgcccgccat catgtggggc
    10741 cgcgatagcc agcggatcaa gttccgcatc gagccggggc agcgcctgcg cgtgcgaggg
    10801 cggctgggcg tctacgagcg cgacggtcgg atgcagctct atgcggattt tgccgagccg
    10861 gcgggcctcg gcgccgaagc gatcgcgctg cagcagctca aggacaagct cgccgccgag
    10921 ggcatgttct caccgcagaa gaagcgaccg ctgccgcgct ttccaaagcg gatcggcgtc
    10981 gtgacgtcga agcatggcgc tgcggtgcac gacatcattc gcacgatcca ccggcgcttc
    11041 ccgacaccga tcctgatcgc cgacgcagcg gtccaaggcg agacggcgcc acaccagatc
    11101 gtgcaaggca tggcaatggt cgtgcgcgcg ggcgtcgacg tcgtcatcgt cggacgcggc
    11161 ggcggggcgg tcaccgatct caccgcgttc aatcacgagc gcgtcgtgcg gacgatctct
    11221 cgatgtccgg tgccggtcat cagcgccgtc ggccacgaag tcgatctatc gctggcggac
    11281 ctcgctgccg atgcgcgcgc gtcgacgcca accgcggctg cggagctcgt cgtcgtcgac
    11341 ggccacgcgg tcgccgatgt cttgcgcaaa gagctgcggc gcctcgatcg cgagatgcat
    11401 cacgtgctcg cgcgcgcgca ccaagacctc gatcgccgga cgatggcgct gcacgcccgc
    11461 ggggaacgcg cactcggagc cacccgtcac cagctgcagc agctgcagca caggctcgcc
    11521 ggcctgcacc cgcgcgcgca gatcctgcgc aggcgcactg tgctcggcga tctcgaacgc
    11581 cgcctcgcat cgctgcaccc gggggctcgc atcgcacgca gtcgccacga cctcgcgtcg
    11641 ctgatcgctc gtcgcgacac ggcgatgcgc gagctcctcg aggagcgccg caccgatctc
    11701 gcgcagctcg gcgcgcagat ggctgcgctc tcgccgctcg cggtcctcga tcgtggctac
    11761 gcgatggtgc gcgcgggaga cgtcatcgtc cgcgacgctg cgcaggtaaa cgcgaaagat
    11821 cgcttgcacg tgacgctggc ccaaggttcg atcgacgtca tcgtcgagaa gatccagaag
    11881 ggccgcgact cgaaatgagc cgcgcgaaat ccaagcgcgc cggcacgcgc ggcggaggcg
    11941 gcacgaacgt cggcaagaag cccacgcgcg cacacctgcc cgacgatctc gccgacgctg
    12001 cggaagcggc actcgacgat ttgctcagct cgtcgcacac gagcctcgac gacgatagcg
    12061 atgagcccgc cactcgcgcg cgcgaaggcg gcaagggtcg cggtctgtcg acgcacgaag
    12121 tctcgctcga agagatcatc ccgatcgatg tcgacatgga cgacgaaggc gacgatgacc
    12181 cgctcacgcg cgcacgccct cgccgcctgc catcgaaggt cgcgccgctc gcgtcggcca
    12241 cgcccaaggc gcccgctccg acgcaaacgt cctcgctcga cgtcaatatc gacacgacga
    12301 accccgtcga gccatccgac gtcgaggagc tcgacgacga cgagccggcg acggcatttc
    12361 aaccaaaggc gtcgcccccg agcaggaatt cgtcgacgca tacgatcgcg cgcgagccat
    12421 cggttgcgaa accgctgcca tccccgccgg ctcgcatttc atcgccgcat gcggtcgcga
    12481 cgcccgcgcg gacggctcat acgatcccgc gtacgggaca gaccccgccg cacggtggtc
    12541 gcgtctcggc gccactcgct cccgaggaca cgtcggccca cacgatcgcg cgcgacgatg
    12601 cgtcgttcgg cgctggctcg tcgaacggcg gatcgccgac gagcgccgcg cgcacgccga
    12661 cgccgcaacc gagccgcgcg ccgcttccgc cgccaagcgg ctcgtcgatg ccctcgtcat
    12721 cgcggcttcc acttccgccg ccgacggaaa gtcgactgca cgggggccgc gtcgatccgg
    12781 aaacgatcga gaaaacgaca ggccgcgaga caaaggctgg tgaggccgat gcgcgcaaag
    12841 cgtggctgca gagcgcgcgc ttcgatcgat cgagtggcga ccacgagccc gcgccgctcg
    12901 acgaggtgga tctcgacagc gagccattcg agcgccccga cgaggatcgc gcgtcgctcg
    12961 accacatcga tctcgatagc gcgccgttcg agcgaagtga cagcgcatcg cccggtcgcg
    13021 ttcccgacag tgcgccgttt ggacgcgttc cggatagcgc atcgttcact cgcgttccgg
    13081 acagcgcacc gttcgagcgc agcggtcgta gcgcgcggtt tgaagtcggc cccctcgacg
    13141 atgcgcttgc cgggctggaa gccgagccgc gtggcgcgag tcagtccggc gaggagcgcg
    13201 cgctgttcga gatgcctgcg cttcgcttcg ccgtgtacga agacccgggc tatctctcgt
    13261 cggcgcagaa cgcgatcgtc gaggcgggtc acgtcgtcgc ggcggcgggc gccggccgtg
    13321 atgcgctgaa gcgtgtcggc gatctcatcc gcacggggag catcgatgtc gtgctcgtgt
    13381 cgttgccagg cggcgagacg atcatcgacg cagccatgtc gctcgagccg cggcgcccga
    13441 cgatcatcgc gtcgctgtcg gcgtcgtcgg tcgaagcggt gagcgcggcg acgctatcgg
    13501 gcgccgatct ggcgacgacg cgcccgcacg acgtcgagcg gatcgctcca ctcgcgttgg
    13561 cagccgcgcg catcgatgcc gagcggcgtg tcgctgcatc ggcaaagggt gcggagcaag
    13621 tcttgcggca gcgcctcgaa gatctcgcgg acgcggagcc cggcggccta ttgccgttcg
    13681 agcactttca gcgcgttctc gagatcgaga tcaagcgcgc aaagcggttc atctatccgc
    13741 tgtcggtcgc gttgttcgcc gtcgaggtca caccaccgcc gccaccggcg ggcatcctcg
    13801 gcatcctgcg cgcgcgcgca ggcaacgcgc tcatcaacac gatccgcgac atcgatatcg
    13861 cgacgcaact cgatcacgag cgcttcctcg tgctgctccc ctatacggat ctcaagggcg
    13921 cagctgccgt cggcaagcgg gtgatcgcgg ccgttgccaa gggccatcct gttgtctccg
    13981 atggccgcac gttcccaccg cgcgtgatcg gcgcgtgcgc cggtatgaag aagggagagc
    14041 ccatcagctt cgcgaagctg atgaaggacg cgacgcgtgc actcgagagc gcgcgacgag
    14101 atggtgcaga cctcgcggtg caaccgtgag caaacccgcc gtcgcgatct ctggcaagac
    14161 gcgcgtcgct gcagtgctcg gctggcccgt cgagcactcg cgcagcccgg cgatgctcaa
    14221 cgccgcattc gcggtcgatc agatcgatgc cgtgctgatt ccgatcggtg tgccgccgga
    14281 cggactgaag agcgtcgtcg ctgcgttgcg cgcgatgaat gtgctcggcg cgagcgtcac
    14341 gctgccgcac aaagtcgcgg tcgctggtct ctgcgacgag ctgtcgcccg aagcgcgcgc
    14401 gatcggcgcg gtcaattgcc tgcaattcac ggaagggcgc ctcgtcggcc acaacaccga
    14461 ctgcgatggg ttcgtcgacg gtctcgtcgc agcgggcttc gagctgcgcg gtaagcgagc
    14521 gatcgtcctc ggcgcaggag gcgcggcgcg cgcggtcgcg catggcctac gcggcgggcg
    14581 agcgctcgag gtcgttgcgc ggcgacccgc cgaagtcgtg tgggcgaaag cctggccgtg
    14641 gaccgacgag caactgcgcg actgcttctc gcgcgcggac ctcgttgtcg actgcacgtc
    14701 cgcgagcctc ggcgatgacg acgcagtgtt cgccgatgcg ctgcccctcg acgcgctgcc
    14761 ggcgcacgcg tgggtgacat ccctgatcta caaccgcaag ccgaaattgc tcgaacgcgc
    14821 gagcgagcga ggtcattcca cgctcgacgg aaaaccgatg ctcgtgcatc agggggctcg
    14881 cgccttccac atctggacgg gcctgccagc tcccgtcgac gtgatggcgc gcgcattaga
    14941 ggcgtcgctg gcctagtcac tgcaaatcgt cctcgcgacc gtcgtggtca tcgaggtgca
    15001 gatgaggaac ttccgtcggt tccggcccgc cccgttcgtc gttctgttgc tgctattcac
    15061 aggtggtggt gtcgcatggg gcgaaaggca tacggccgac ctctacatcg aggaccccta
    15121 cgcgccgcat cacacgaccg gatccgaggc aagactcggc acggcggtcg gcttcctcta
    15181 caacgagccg gtcgatgtca ccgcgctagg cgcgtcggcc gccatcggct atcggtgggg
    15241 aagatttacc gtcgagagtg agtacacgta cctcggcttc caggtgcgcg gtcctgacaa
    15301 cacgcggctg ggtgcggggc agcgcctggc gttcctcgga cggtttgacg tgattcgcct
    15361 cggcccgcgc atcgtcggcc agaactcgct gctctcgatc tatatcgagg gtggcgcggg
    15421 cacatcgtgg aaccggtggt ggcgtccggc gtatgacgaa gccagtcgca tcgtcccgaa
    15481 cgacacgcgg cgcgcggaag gccaggtcgg gttcggcatc gcgatcgatc accgcctgca
    15541 ggaaccgatc ggtttcccac gtcgcgtcgg ttggttcctc ggctggcgcg tggcgatgtc
    15601 gccgcatcag ccgatggcag catcggtgtg ccgcggcgtg acgtgctcgc cgattccgat
    15661 gatggacgaa gaccgcctcg tcgatcgctc gatgctcttc cagtcgagct tggcgtttac
    15721 gttttagcta ctggatcgtg ccggtcgacg gctcgagcac cggcggaggc ggaacgccgt
    15781 cgttgctcgg ctgctgctgc gtcgtgtcgc cgccctcttg gccgttgcac tgcacgtcat
    15841 cgacgcagtc gccgcggttc gaggagcact ccatgcggtt cgcctgcgcg tagaccggga
    15901 cgatctcttt ctcggcggtc gtcgggatcg tcttgcagca gcacgtcggc gacgtgtcgc
    15961 cttcgttgac catcgagctc tggcccttcg gcttaccgcc accacacgcg aacgcgagca
    16021 ccgttaccgc gaacaagctg aggatcctca tgcggtggtc gtaacacgac cgctagacgg
    16081 cgcgccacga cgccgtgagc accccgacaa tcgccccgaa ccaaatcgtg cctgccgcca
    16141 tccaagccag cgccgatacg acgggcgcga tttcgaggcc gtcctcctgc gggttccacc
    16201 gccacaggat cagcagcagc gccgcgctgg cgagcaggcc gagagccacc cagtgctcct
    16261 tgatgtcgaa ccagcgggcg gcttcggcgg cccggcgcac catctctctc gtcgcgggtg
    16321 actcgtaggc ctgaccccct tcgcgtgcgg tgatcttgcc gagctcgcgc tcgtgcgttg
    16381 ctgcttcacc gctgatcgcg acctggttct cgagatacgc ggcgcggacc tcgaccttgt
    16441 atgtcgggta catgatgttg ccggcgacga acgcgccgag ctgaaacgcg aggacgatcc
    16501 acgcgaatcg ccgcaccgct cgtcgcttcc cgtattgccc gcgcagatag cgtcgcgtcc
    16561 acagcaccag atgcgtcgat gcaccgaccg cggtcacgcc aagcgcggtg tggagcacca
    16621 gcagccagcg cgcagtcgta tcgagcagca ccgagcgacg ttaccccgaa aacgccaaag
    16681 gcccgaacat cgggcccgcg gcagggagga tcaagctaag cgaaaagcga aaactaaccg
    16741 tgcgtcgtgt tgtgacgacg aatgaacgtc ggcacgtcga gctcgctctc ggtatcgatc
    16801 tcaccggcga gcacggcctt catcgagggg cgcgattgtg cgcgctttcg atcgggctga
    16861 acgagggggt tgccgtcgac gggtgtcgcc ggacccgagc ctgcagccat tctctggctc
    16921 ggctcgccct cgggaacatt gagatcttcg agtgagcgat acgaatcgtg catcggcgag
    16981 acctcgacgt ccggcgatgc ccactcgatg tgaacgtccg gctcgtcgac gacgtgcgcc
    17041 tgcatcttcg gttgcgaagc gcgcggcggc atcggaggcg gatactcttt ggtgatctgc
    17101 gtcgagacgt catacggcag cgagacctgc gatgcggtcg ccttgtacgt cggcttgctc
    17161 gcggcctgca tgcgcgtctg aacctgcggc tcgacgggaa gcgtcgcggc agcgcgatcg
    17221 aagcccgtcg cgatcacggt gatgcgaacc tcgtcgctga ggttcggatc gatgaccgag
    17281 ccgaagatga tgttcgcgtc ctcgtgcgcc gcctgctgga tcagcgagga ggcctcgttg
    17341 acctcgtgca gcgtcaggtc gggaccgccc gtgatgttga tcaggatgcc ggtcgcgcct
    17401 tcgatcgaga cgtcctcgag gagcggcgag ctgatcgcca tctcggcggc ttcacctgcg
    17461 cgcttcttgc cctgaccgat gccagtgccc atgagcgcgc ggcccatgcc cgccatgatc
    17521 gtgcggacgt cggcgaagtc gacgttgatg aggccgggga ccgtcatgag gtccgagatg
    17581 ccttgcacgg cgttcaggag gaccgagtcc gccttgcgga acgcttcgac catcgacgtg
    17641 ttctggccga cgagcgcgag caggcgattg ttcggaatga cgatcagcgt gtcgaccgct
    17701 gcgccgaggc gatcgatgcc ctcgaccgct tggcgtgcgc gcttcttgcc ttcgaagccg
    17761 aatggcttgg tgacaacgcc gaccgtgagc gctccgcagt cccgcgcgat ctgcgcgatg
    17821 actggagcag cgccggtgcc ggtgccgccg cccatgcctg ccgtgacgaa gaccatgtcg
    17881 gcgccgctga tgaggtcggc gatctgctgc atggattcct cggccgagcg ccgtcccact
    17941 tcgggattcg cgccagcgcc aaggcccttg gtcagagcat taccgagctg gatcttgtgc
    18001 ggcgccatgt tggcttcgag cgcctgcatg tcggtgttgg ccacgacgaa ctcgacgccg
    18061 tcgagattgc cgctgatcat ggtgttgacc gcgttaccgc cgcctccgcc gacaccaatg
    18121 acaaggattt ttgcgtgact gacgtcgtcg aattcgatga gtgccatgga tcgattgccc
    18181 cctggtaaac ccatatgaaa gagggtgatc atcaggggca actttttgat cgccgggggg
    18241 atcggatgaa tcgtgccatc gtgatcgttt tgttcgctgt cgcgtgtggt gacgatgcct
    18301 cgtcgagctc cgacgcgggc ggcgatggac gcaacctcag cggcgacggc aacggccaat
    18361 tcggcaacgg cccgacgctg ggcggctgtc ccgtgctccc cgacaatcac atcttcaaca
    18421 cgaagatcga tcagttgccc gtcgatccga attcagccgc gtacatcgcg acgatcggcg
    18481 gcacgacgag gctccacctc gatctcggcc agcagacgga tcaatcgcag gccgacttct
    18541 acggcatccc gtacaacgtc gccgcgatgt cagcagcgac gtggcccaca gtggcgtaca
    18601 cgtctcccga tccaggcatg acatgggacc cgaagccgga gtcggattgc gctgacgctg
    18661 cgcactcgat cgtctcgccc tgcgattcga tcgcgcagcc gaagattccg atcatgacga
    18721 caccgctcgt cgagggcggc atcaacacgg cgtcgagcca catgccatac ggcgaccacc
    18781 acatcctgat cctcgacacg gacagctgcc tgctctggga aacgtaccac tcgtacttcg
    18841 tatcgagcgc gtggcagatc ttcggctccg ccgtctggga catgcgctcg aacaacctgc
    18901 gtcccgactc gtggagctcg gctgacgccg cgggctttcc gattctcccg ctgctgctca
    18961 aagagcccga agcgacgagc ggcacgatca agcacgcgct gcgcttcacg atcgacacgc
    19021 cgaagattcg cggcgtctac acatggccag cgacgcacga agcgtcgaac gtgacgggcg
    19081 cgggcaatcc gccgtacggc cagctgttcc gcctcaaggc gagctaccag atcccggcga
    19141 actacaacgt gcaatccaaa gcgatcctga ccgcgatgaa ggagtacggc atgtacatcg
    19201 ccgacggcgg ctcggacatg tacgtgaccg gtgcgccgag cgccaactgg atggactcga
    19261 cattctccga ggtgcagtcg gtcgcggcat cgaacttcga agccgtgagc ctcgcgccga
    19321 tcaaagcgcg tgcgggctgg tcggcgacgt cagctgccgt tccggcgccc tgacgatcag
    19381 aacatctcgg cgagacgacc ccaggcacgc ttgaacaggc ctggacgatc catctcgcgc
    19441 gatggcgtct gcgcgctgcg accgtggtcg gcgccgaaca tcacgaggcc aacacccgtc
    19501 gcgtatgcgg gcgagcgcac gacatcgacg aggccaccga tcttcgtcgg tacgccgcgg
    19561 cgcgtgggaa gaccgagcgt gttctcggcg acgtcgcaga cgccgtccat cgcggtggcg
    19621 ccacccgtga ggacgatgcc gccgccgagg ctgtcgtagt agccggagcg catgagctcc
    19681 ttcttgacgt gctcgaagat ctcttcgacg cgcggctcga tgacttcgac gagctgcatg
    19741 cgcggaagga cgcgaggccc gcgaccgccg accgatggga cttccatcgt ctcgctgtcg
    19801 tcgacgtggc cacgccatgc gcagccatac ttgcgcttga tcttctcggc gctgtcgagc
    19861 ggcgtgcgca ggaccgtcgc gatgtcgttc gtgatgtgcc cgccgccgag cggcaggacc
    19921 gacgtgtgca cgatcgcgcc gtcgcagtag atcatcaggt cgcaggtgcc accgccgatg
    19981 tcgatcagcg cgacgccgag ctctttctcg tcgtcttcga gcacggcctg cgcggacgcg
    20041 agggactcga ggacgatgtc ggcgacttgg aggttgcagc ggttcgcgca cttgatgacg
    20101 ttctgcgcgg acgcgaccgc ggtcgtcacc atgtggactt tggcttcgag gcgaacgccg
    20161 gccatgccga gtgggtcccg gatgccatct tgatcgtcga cgacgtactg ctgcggcagg
    20221 acgtgcagca cttcgcggtc catcgggatc gcgacggcct tcgcttgctc gatcacgcgc
    20281 gcgatgtcgg cttcgcggac ttccttgtcc ttaacggcga tgatgccgtg cgagttgagg
    20341 ccgcggacgt gcgcgccgga gatcgcggcg tagacgctcg agatctcgca gccggccatg
    20401 ttctcggctt cgtcgacggc ttgctggatc gccgtcaccg tcgcgtcgat gttcacgacg
    20461 tagccgcggc gcaggccctt cgagggggcg gtgccgatgc cgatgatgtc gacgccatcc
    20521 tccgtgacct cacctgcgat gcaggcgatc ttggtggtgc cgatgtcgag gccgactacg
    20581 atctcgttgc tcttgggctt ggccatgtgg agttactgat ctttctctgg ggcgaaggcg
    20641 acggtgacct gatcggaatg ggcatcgagg tgaatggcgc gcatgcgcga gcgctcgctg
    20701 ctcgagagct cgttccacgt cgcgtcgaac gtggcgaggc gcgtggcgag gaagtcattt
    20761 gcaacgccga gcctgatctt gatcgcgtgc tcgtacgtga cgagcgtgag gttgccgtgc
    20821 ggatcgagat gcagctcgcc gatcgacgga cggtcgctgc tgtcgttcca gcgagcgagt
    20881 gcgtcgagcg cagcgcgaac ctgtgcggcg ccaccgtcgg gatttgcgtt gaacgtcgcg
    20941 cgatcgaggc cggtgatgat cggcaacccg gcgccctcgt cgagggtgag gtcggctttc
    21001 ttgaacgggt gcccggcctc gtcggcgagg tagagaccgg acagctcgac gacggcggcc
    21061 gcgacgtgct cgcgcacgct gaccgtgatc gtgtgaggca ggacgcggtg cgcttcggcc
    21121 tgcgcgatcc acggattcgt cttggccgcg cgtgcgacct cgtcgagatc gaccgcgaac
    21181 acgttgtcac cgacgtgggc cggaaccgtg ctcgcgatct cgtcggtcgc aacctggcga
    21241 ttgccgctga catcgatgtt cgtgatcgcg aatcgcggcg agtgagtgac gaagcgatat
    21301 cccgcccacg ccgtgccgcc gaccgtgccg aggaccgcga ggccgatcag cgcggggacc
    21361 gaccggcgga gcacgcgcga gcacgcgtcc gcgatcaccg cgggcttcgg aagcttggac
    21421 cagaccgatc ccggctgcgt cttgcggcga ttaccgcgac gcttgaacgt cagtcgggag
    21481 gcacttggcg gagccgcctt tgccccatcc cgtcgtgccc cgagggatcg atccatgctc
    21541 tgcaaatacg catgctggga tcacgggggc aatttttcgg cgccactccg gatctcggag
    21601 tcgctttgcg cagatgcacc tactcgatgt cgtcgtcatc cgtgatcttc acgaagtcct
    21661 tgtccgagtc tttcgagctc gagcccgacg cctgatcatc ggaacgggac gagctctttt
    21721 tcgacttggc cgtgacgcgc ttcttgccgg gcgacgtgcg cgcagccttc ggcttctttt
    21781 ccgagctgtc gctgctcgcc ttctcgggct ttgccttggt tgtcttcgtc gtcttcggct
    21841 tgaagacctg cgcctcggcc gacgttgcgg tcagaccgag cacgaggaag agaccgagcc
    21901 agcgcgacat cccggttatc ttgtcacgca aggggggcgt tcgaccactt cgagccgccg
    21961 cagctgatcc cccttgaatg taaagcggaa ttcccggtcg tcggagcggc agaccgtgcc
    22021 cgtgcagtgg gccatggccc gaaccagatc ttcgagctcg ggcaagacca cctcggtgcg
    22081 tggaacgacg accgtcgtgc gctgcgtgtg cgggtcgcca tcgccgtcgt cgtccgtctc
    22141 gctgtactga tcgtacgtca cggtcaccct ctcgaggccc ttcgccg
//
LOCUS       DATLBZ010000607        10021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3493483, whole genome shotgun sequence.
ACCESSION   DATLBZ010000607 DATLBZ010000000
VERSION     DATLBZ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3493483"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..616
                     /locus_tag="VMZ53_29775"
     CDS             68..616
                     /locus_tag="VMZ53_29775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32741.1"
                     /translation="MLSTQTTHATSSTRSSVHSLDSLAQRSSDELDALYRAATVSQSM
                     HAADGALVGRMLAVRGVRGPLASSLRRFAGSRSFVWEGKTFQASGDSTGVGHNRVFIP
                     GVFGRQNLFPFETSFGPSAIDGKPTLILDYDLDVNPGYIRKIHDEIREVAPGLFLGPA
                     MWKGARDKKTLVLWFALDSRLS"
     gene            622..1566
                     /locus_tag="VMZ53_29780"
     CDS             622..1566
                     /locus_tag="VMZ53_29780"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS32742.1"
                     /translation="MRTSQRSSVAGVARTNGRRGAHGTTRPAPGHVVITGAAGAIGSA
                     LARAMRAAWPDVELALVDRANGGGSSHESSSGEWATSGAAARIAAEVGGTAYGADLAD
                     VDALPQLVARIEENGPIDALVNCAGVMRVRDVSTWRWEDAHELIAIDLLAPLRLQDLV
                     VRRMLKRRDEHAGGGGDGGSRDGGRRGLIVNIASMAGRVPLKGCAYYGAAKAGLAMAS
                     EIARADLAGRGVQVVTVYPGPVKSALEAGARADYGGAGLVGRFAPTGDPSVLASKIMR
                     AIDEGQPRVIYPGLYGVGWTAPNLSRWVALAFGPSPAS"
     gene            complement(1612..2511)
                     /locus_tag="VMZ53_29785"
     CDS             complement(1612..2511)
                     /locus_tag="VMZ53_29785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS32743.1"
                     /translation="MNVGPLARARLAEAELVLADACAFDRATEVADEKLFGPGPTSAA
                     QAFGAWDGNELIGVGSVAARWLRLIAVTPRARTSGAGSALLAAIEAAARADGQTRLSA
                     LDQPGNYLAPGIDERNAHAIAWLERRGFTRGADLRTNLLLDVRQNPRISRERAAEAAE
                     LAHAHGYEVRRAKPAERQHLLDAIAGEFGGAWPYEVERALGHAPAAIHVACKDGAYCA
                     FAAHDGNNRGLGWFGPAGTWKAHRGKGLGEALLLACLVDVAAEHSQCEVAWIGPRPFY
                     EKVVGAPGIVGERRFVSLTKLLG"
     gene            2620..3588
                     /locus_tag="VMZ53_29790"
     CDS             2620..3588
                     /locus_tag="VMZ53_29790"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HUS32744.1"
                     /translation="MRAMLLCAGLSTRLGKLGAECPKPLLPVCGIPILHFGIANLVAH
                     GIRDLVVNTHHRADFIREALGDGSRFGARIQYSHEPAILGTGGGLKHALPLLDPDGRD
                     EPFVSVNGKLIFDLDFTALFDAYRRAGDIAGMMVVRRVPDAKAWGAVDVRFDERGPYV
                     VNVLGDGEHMFCGVHVTRPSVMARLPDGESDSIRQGYLPWMHAGERVAAYEHDDGYFA
                     EHSTPERYLESSYALLSGTRLRNPPGALTGRDPSATIDPSATIIEPVRICAGASVGPG
                     VTVGPRVVVGPGARVTESASDTVVWANASLPGGTVEQFGNGRVVTP"
     gene            complement(3585..3953)
                     /locus_tag="VMZ53_29795"
     CDS             complement(3585..3953)
                     /locus_tag="VMZ53_29795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32745.1"
                     /translation="MRLLAIALVLAAATGCKAKEPSARKKKPVATMTEVELKRGEDAC
                     KAYVDKVCACATQKPDAAELAKECQLAKALPEALQIALSVAINPESKPDVVEQSYDSV
                     RTTVSQCIQETAKLPAAGCN"
     gene            complement(3950..4744)
                     /locus_tag="VMZ53_29800"
     CDS             complement(3950..4744)
                     /locus_tag="VMZ53_29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HUS32746.1"
                     /translation="MSTSFPLENARVRFAGDTNIGRKRDHNEDSIALPDSGERIAIVC
                     DGMGGHASGEVASRLAAELIVDHFVETGKQQVLTWPYKVDRDLRKDVNRMITGIMLAN
                     LEIWERSQRESRFKGMGTTCVALYFLDDHLIIGHVGDSRAYRVRGTELTQMTEDHSLI
                     NDYIRMKRVTPEEAENWPHKNVIVRALGMKESVQVDILTEVPRVGDTYMLCSDGLTGM
                     LKDDTIQHILMSERDLDRAVDRLITAANEEGGVDNISVVLARIEAS"
     gene            4939..6903
                     /locus_tag="VMZ53_29805"
     CDS             4939..6903
                     /locus_tag="VMZ53_29805"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HUS32747.1"
                     /translation="MTILAQGSVTDRPWGMTLGALGMRGLSGQVAIVSDGKQYIVAFD
                     QGVIVAAYSPLASDAAVRLALTSNLITSTQVSDITRRMAAAPQRDEIDLLAELVRLTP
                     DQAARLRRRLVAQRAARTFTPGYGDFVVEDRVQIPIIPGGELDVRTVIYLGAKTNLSE
                     DRLTTELAQLGTYFELKREAVDDLQQFGFTSAEKPVLDMLLEGAYVDEIIAAQPALGE
                     RVVRAIAYSLASCGACKLAAPPRAARAVTPSTAPTLQQSRTTTQPRNAPPGQPATPRT
                     STGNAMPTMSRTATGVPTPIAQQGSGAVPHPAASYGSGAVPRPVAAAPSGAVPRPISS
                     APSGATPSRPRTPSTPPPGTENFARSRTPSVPPPMSHASVPVPSRGRPPSSPPPPSSA
                     LDAPTSPGRRSPPPRRSRRDSSAVTEIEHLLETKIPLLDRGVDHFTLLGLSLEASPAD
                     IRSAYFTLARKLHPDRLSAIGVEDEERRAQRLMAQVNLAFAVLNDPVKRNEYTSIVHR
                     GGEAAVKAEEARADELAMRVMRAEEAFKQGEMALRRDQLQQAIEQFQMAVELQPREAE
                     YQALLAWAQFAIAPDKTKIANETRKALLRAADANDRSPSAKFYLGRVERMLGREREAL
                     QYFQEVLAIKPSHVEAASEARILQQRLNKR"
     gene            6900..7130
                     /locus_tag="VMZ53_29810"
     CDS             6900..7130
                     /locus_tag="VMZ53_29810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32748.1"
                     /translation="MTKLVLVEGSGVHDIFDVVELTETLARVRSPFLFEIGEELKVEV
                     ERDGVTKELVARVVSHSGPGDAKVTELELVSP"
     gene            7127..7414
                     /locus_tag="VMZ53_29815"
     CDS             7127..7414
                     /locus_tag="VMZ53_29815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32749.1"
                     /translation="MMIGDLDDVQYDLELDGELVRKQLARRVWEHRGWATVAIAFQER
                     DKAGEWKPAKLALLRFRHEREAWKKQAAVTIDGADALALAETLESWREHLG"
     gene            7480..8217
                     /locus_tag="VMZ53_29820"
     CDS             7480..8217
                     /locus_tag="VMZ53_29820"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HUS32750.1"
                     /translation="MLKPVEKQRVAEEIAEQLRSLILNGQYPPGSKLPPERELSKRLR
                     VNRASLREALKKLEHLGLVRIRQGDGTRVQNFMETGGIELVQHLLPLGGGKPELIRDL
                     LEFRRIFGRELARLAAARSGKDRDGLAKLRALADKADNTTSAADLFDLDFDFYVAIAS
                     MSGNQVMLLLLNTVRDGVRGFMPLLANLAGPGDQVRKHHRELITAIEKGDITSAARIA
                     DEYLKMGAELAARMGGRPELQPQPLPE"
     gene            8230..8520
                     /gene="gatC"
                     /locus_tag="VMZ53_29825"
     CDS             8230..8520
                     /gene="gatC"
                     /locus_tag="VMZ53_29825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HUS32751.1"
                     /translation="MTRKEVEEIALLARLHLEPAELTSLEQELGAILEHFSTLASVDT
                     TDVPPMTHAVPLDLRLRSDEVAPSLTQAEALANAPAKQGDVFVVPAIITEKE"
     gene            8517..9977
                     /gene="gatA"
                     /locus_tag="VMZ53_29830"
     CDS             8517..9977
                     /gene="gatA"
                     /locus_tag="VMZ53_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HUS32752.1"
                     /translation="MSEWLSVAETAQKIAAGELTAVRVIDRYIERIRDVDRGLKAYLT
                     LDAERARAEAVAIDKRRAAGERLPRLAGVPISLKDVLVTRGMPTTAGSKILESFVAPY
                     DAACVEKLKAAGAIMLGKVNCDEFAMGSSTENSAYQQTKNPWDPQRVPGGSSGGSSAA
                     VAAGLCAASLGTDTGGSIRQPAAFCGVVGLKPTYGRVSRWGVVAFASSLDQVGPITRT
                     VQDAALLLEVIAGFDPRDSTSLDEPVPQYSEALVGDVTGLRIGLPHEYFGEGIDPEVR
                     AALDKTIAVLKERGAITVDITLPHTHLALPAYYIVAPAEASSNLARYDGIRFSKAASG
                     AKDLLDLYMKSRGAGFGREVKRRIMLGTYALRSGYYDAYYKKAQQVRTLIKRDFDKAF
                     QYCDVILTPTTPTPAFKRGSKSNPLDMYLGDVFTLSCNLAGLPGLSVPCGLTSEKLPV
                     GAQLLGKPLDEATLLRVGHVIESLCGLRGQIPGGMS"
BASE COUNT         1591 a         3331 c         3416 g         1683 t
ORIGIN      
        1 ggtgctgccg cctggcgcct catcgctcct gatgtgaccg cgaaacgccg caagaaaggg
       61 acctgacatg ctctcgacgc aaacgaccca cgcgacttcc tccactcgtt cgtccgtcca
      121 ctcgctcgat tcgctcgcgc agcgcagctc cgacgagctc gacgcgctct accgcgcagc
      181 gacggtctcg cagtcgatgc acgccgccga tggtgcgctc gtcggtcgca tgctcgccgt
      241 gcgcggcgtg cgcggcccgc tcgcgagctc actgcgccgc ttcgccggct cgcgttcgtt
      301 cgtctgggaa ggcaagacgt tccaagcatc gggcgactcg acaggcgtcg gccataaccg
      361 cgtctttatc cccggcgtat tcgggcggca gaacctgttt cccttcgaga cgtcgttcgg
      421 cccgagcgcg atcgacggca agccgacgct gatcctcgac tacgacctcg atgtgaatcc
      481 cggctacatc cgcaagatcc acgacgagat tcgcgaggtc gcaccggggc tcttcctcgg
      541 tcccgcgatg tggaagggcg cgcgcgacaa gaagaccctc gtcctgtggt tcgcgctcga
      601 ctcgaggctg tcgtaacgct catgcgaaca tcgcagcgga gctcggtcgc aggtgtcgcg
      661 cggacgaacg ggcggcgtgg tgcacacgga actacgaggc cagctcccgg gcacgtcgta
      721 atcacgggcg cggcgggcgc gatcggctcc gcacttgcgc gcgcgatgcg ggcggcttgg
      781 cccgatgtcg agctcgcgct tgtcgatcgc gcgaacggcg gcggctcgag ccacgaatcg
      841 tcgagtggcg aatgggcgac aagcggagcg gctgcacgta tcgcagcgga ggtcggcggc
      901 accgcgtacg gagcggacct cgcagacgtc gatgcgttgc cgcagctcgt cgcgcgcatc
      961 gaggagaacg gtccgatcga cgcgctcgtg aattgcgcag gcgtgatgcg cgtccgcgat
     1021 gtatcgacgt ggcgctggga agatgcgcac gagctcatcg cgatcgactt gctggcaccg
     1081 ttgcgactgc aagacctggt cgtgcgcaga atgctgaagc gtcgcgatga gcatgctggc
     1141 ggcggtggcg atggcggtag tcgcgacggc ggcaggcgcg ggctcatcgt gaatatcgcg
     1201 agcatggctg ggcgcgttcc gctgaagggc tgcgcgtatt acggcgcggc gaaggccggc
     1261 ttggcgatgg cgtcggagat cgcgcgcgcc gatctcgcag ggcgcggcgt gcaggtcgtg
     1321 acggtgtatc cggggcccgt gaagtcggcg ctcgaggccg gggcgcgagc cgactatggc
     1381 ggtgccggat tggttggacg gtttgcgccg actggcgatc cgagcgtgct cgcgtcgaag
     1441 atcatgcgcg cgatcgacga ggggcagcct cgcgttatct atccggggct gtatggcgtc
     1501 ggatggaccg cgccgaacct gagccggtgg gtcgcgctcg cgttcggacc gagccccgcg
     1561 tcgtagggat gtgccgggct cgtagtccgg cttcgcgatc ccgagcgtcc attagccgag
     1621 caacttcgtc agcgacacga agcggcgttc gcccacgatg cccggcgcac cgacgacctt
     1681 ctcgtagaac gggcgcggcc cgatccacgc gacctcgcat tgcgagtgtt cggcggcgac
     1741 atcgacgaga catgcgagca ggagggcctc gccgaggcct ttccctcggt gcgccttcca
     1801 cgtgcctgcc gggccgaacc agccgagtcc gcggttgttg ccgtcgtgcg cggcgaatgc
     1861 gcagtacgcg ccgtctttgc atgcgacgtg gatcgcggcc ggggcgtggc cgagcgcgcg
     1921 ttcgacttcg tatggccatg caccgccgaa ttcgcccgcg atcgcatcga ggaggtgctg
     1981 gcgctcggcc ggctttgcgc gacgcacctc gtagccgtgt gcgtgggcca gctccgcggc
     2041 ttcggcggcg cgctcgcgcg agatgcgcgg attttggcgg acgtcgagca agaggttcgt
     2101 gcgcaggtcc gcgccgcgcg tgaagcctcg acgctcgagc cacgcgatcg cgtgggcgtt
     2161 gcgctcgtcg ataccgggcg cgagatagtt gcctggttga tcgagcgcgg agaggcgcgt
     2221 ttggccgtcg gcgcgcgcgg ccgcttcgat cgctgcgagg agcgccgagc ctgcgccgga
     2281 cgtgcgggcg cgcggcgtga ccgcgatcag gcgcagccag cgtgcagcca cggagccgac
     2341 gccgatcagc tcgttgccgt cccacgcgcc gaacgcttga gccgcggacg tggggcctgg
     2401 accgaagagc ttttcgtcgg cgacctcggt cgcgcgatcg aacgcgcacg cgtcggcgag
     2461 cacgagctcg gcctcggcaa gacgcgctct cgccagtggc ccgacattca cgcgatcacc
     2521 gtagcactcg catcgaggct gaccacattc tcgcaaacgg cgcgcatcga cgccgatcac
     2581 attctcgcaa accgcgcctc gctatgcgac ggtctcggca tgcgcgcgat gctgttatgc
     2641 gccggcttgt cgacgcgact cggcaagctc ggagccgagt gcccaaagcc actcttgccc
     2701 gtgtgcggca ttccgattct gcatttcggg atcgcgaatc tggtggctca tggcattcgc
     2761 gacctcgtcg tcaacacgca tcaccgcgcg gacttcattc gcgaagcgct cggcgacggc
     2821 agccggtttg gcgcgcgcat tcagtacagc cacgagcctg cgatcttggg caccggcggc
     2881 ggcttgaagc acgcgttgcc gctcctcgat cccgatggtc gcgacgagcc gttcgtgtcc
     2941 gtcaacggca agctcatttt cgatctcgat ttcaccgcgc tgttcgatgc gtatcggcga
     3001 gcgggggaca tcgcgggaat gatggtggtg cgccgcgtgc ccgatgcgaa agcgtggggc
     3061 gccgtcgacg ttcgcttcga cgagcgaggg ccgtacgtcg tgaacgtcct cggcgatggc
     3121 gagcacatgt tctgtggcgt gcacgtgacg cgtccgtcgg tgatggcgcg gctgccggat
     3181 ggcgagagcg actcgattcg ccagggctat ctgccgtgga tgcacgccgg cgagcgcgtc
     3241 gctgcctacg agcacgacga cggctacttc gccgagcact cgacgcccga gcgctacctc
     3301 gagtcgagct acgcgctgct ctccggcacg cgcttgcgca atccgccggg cgctctcacc
     3361 ggccgcgacc cgtctgccac catcgatccg tcggcgacga tcatcgagcc cgttcgtatc
     3421 tgcgcgggcg cgtccgtggg gcccggcgtg acagtcggcc cgcgcgtcgt cgtcggtccg
     3481 ggcgcgcgcg tcaccgaatc agcgagcgac accgtcgtgt gggcgaacgc gtcactgccc
     3541 ggcggcaccg tcgagcaatt cggcaacggc cgcgtcgtga cgccctagtt gcaaccggcc
     3601 gctggcagct tcgcagtctc ttgaatgcac tgcgataccg tcgtgcgcac gctgtcgtac
     3661 gactgctcga cgacgtcggg cttgctctct ggattgatcg cgaccgagag cgcgatctgc
     3721 agcgcttcgg gcagcgcctt ggcaagctgg cattccttcg cgagctcggc ggcgtcaggc
     3781 ttctgcgtcg cgcacgcgca gaccttgtcg acgtacgcct tgcacgcgtc ctcgccgcgc
     3841 ttgagctcga cctcggtcat cgtcgcgacc ggcttcttct tcctcgctga cggctccttc
     3901 gccttgcagc cggtcgcggc cgccagcacg agcgcgatgg cgagcagcct cacgacgcct
     3961 cgatgcgcgc gagcacgacc gagatgttgt cgacgccgcc ttcttcattc gcggccgtga
     4021 tcagccgatc gaccgcgcgg tcgagatcgc gttccgacat caggatgtgc tggatcgtgt
     4081 cgtccttgag catgcccgtg aggccgtcgg agcagagcat gtacgtatcg ccgacgcgcg
     4141 ggacctccgt aaggatgtcg acctgcacgc tttccttcat gccgagtgcg cgcacgatca
     4201 cgttcttgtg cggccagttc tcggcttcct ccggcgtcac ccgcttcatg cggatgtagt
     4261 cgttgatcag cgagtggtct tccgtcatct gggtcagctc ggtgccgcgt acgcgatagg
     4321 cgcggctgtc tccaacgtga ccgatgatga ggtgatcgtc gaggaagtag agcgcgacgc
     4381 acgtcgtgcc catgcctttg aagcgcgact cgcgttgcga gcgttcccag atctcgaggt
     4441 tcgccagcat gatgccggtg atcatccgat tgacgtcttt gcgcaggtcg cggtcgacct
     4501 tgtacggcca cgtcaggact tgctgcttgc cggtctcgac gaagtggtcg acgatcagct
     4561 cggcggcgag acggctcgcg acctcgcccg atgcgtgacc gcccatgccg tcacacacga
     4621 tcgcgatgcg ttcgcccgag tccggcagcg cgatcgaatc ctcgttgtga tcgcgcttgc
     4681 ggccaatgtt cgtgtcgccg gcaaaccgca cccgtgcgtt ttcgagcggg aacgaggtac
     4741 tcacctccga gaattctacc agtgccggcc gcattgactc cactctggtg tctgaccatc
     4801 ctctagggga atgaatggct tgcccgcagc cggctggtgg ggctacgcgg agggcaaccg
     4861 gaccgcggtc ggtatcgtcg tgattgttgt agcggttgca gccattctgt tcgtgacccg
     4921 ccactggtgg aggcgccggt gaccatcctg gcgcagggct ccgtcaccga tcggccatgg
     4981 ggcatgacgc tgggtgcgct cggcatgcgc gggctgtcgg gccaggtcgc gatcgtgtcc
     5041 gacggcaagc agtacatcgt cgcattcgac caaggcgtga tcgtcgccgc gtactcgccg
     5101 cttgcgagcg atgccgccgt tcgcctcgcg ctgacgagca acctcatcac ctcgacgcag
     5161 gtcagcgaca tcacgcgccg catggctgcc gctccacagc gcgacgagat cgatctgctt
     5221 gccgagctcg tgcgcctcac gccagaccaa gcggcgcgcc tgcgacggcg tctggtggct
     5281 cagcgagcag cgcgcacctt cacgcccggt tacggcgatt tcgtcgtcga ggaccgcgtc
     5341 cagatcccga tcatccccgg cggcgagctc gacgttcgca ccgtcatcta cctcggcgcg
     5401 aagacgaacc tgtccgagga ccggctgacc acagagctcg cgcagctcgg cacgtacttc
     5461 gagctcaagc gcgaagccgt cgacgatctg cagcagttcg gcttcacgtc agcggagaag
     5521 ccggtgctgg acatgctgct cgagggcgca tacgtcgacg agatcatcgc cgcgcagccc
     5581 gcactcggcg aacgcgtcgt gcgtgcgatc gcgtactcgc tcgcgtcgtg tggcgcatgc
     5641 aagctggccg cgcccccacg tgcggcgcgc gccgtcacgc cgtcgacggc tccgacgctt
     5701 cagcagtcgc gtacgaccac gcagccgcgc aacgcgcccc cgggccagcc cgccacgccc
     5761 cgaacgtcga cgggaaatgc gatgccgacg atgtcgcgca ccgcgacggg cgtgccgacg
     5821 ccgatcgccc agcagggctc gggcgcagtt ccgcatcccg cggcgagcta cggatccggc
     5881 gcagtgccgc ggccggttgc agcggcgccg tcgggcgcag tgccgcggcc gatctcgtca
     5941 gctccgtcgg gggccacgcc gtcgcgacct cgcacgccga gcacaccacc gcctggaacc
     6001 gagaacttcg cgcgcagtcg tacgccgagc gtgccgccgc ccatgtcgca cgcgagcgtg
     6061 cccgtaccgt cgcgaggtcg accgccgagc agcccaccac caccgtcgag cgcgctcgat
     6121 gcgccgacaa gtccgggccg tcggtctcca ccgcctcggc gaagccgccg cgactcgagt
     6181 gcggtcaccg agatcgagca cctgctcgag acgaagatcc cgctactaga taggggcgtc
     6241 gatcacttca cgctgctcgg cctctcgctg gaagcgtcgc cagcggatat ccgcagcgcg
     6301 tacttcacgc tcgcgcgcaa gctgcatccc gatcggctgt ccgcgatcgg tgtcgaggac
     6361 gaagagcgcc gcgcgcagcg cttgatggcg caggtcaacc tcgcgttcgc cgtcctcaat
     6421 gatccggtga agcgcaacga gtacacgtcg atcgtgcacc gcggtggcga agcagctgtg
     6481 aaggcggaag aggcgagggc cgacgagctc gcgatgcgcg tgatgcgtgc agaggaagcg
     6541 ttcaagcagg gcgagatggc gctgcggcgc gatcaactgc agcaagcgat cgaacagttc
     6601 cagatggcgg tcgagctcca gccccgcgag gccgagtacc aagcgctgct cgcgtgggcg
     6661 cagttcgcga tcgcgccaga caagacgaag atcgcaaacg agacgcgcaa ggcgctgctg
     6721 cgcgctgcgg acgcgaatga tcgatcgccg tcggcgaagt tctatctcgg ccgcgtcgag
     6781 cgcatgctcg gccgggaacg cgaggcgctg cagtacttcc aagaggtcct cgcgatcaaa
     6841 ccgagccacg tcgaggcggc gagcgaggcg cgcattctcc agcagcgctt gaacaagcga
     6901 tgacgaagct cgtgctcgtc gaggggtctg gcgtccacga catcttcgac gtcgtcgagc
     6961 tgacggagac gctcgcgcgc gttcgctcgc cgtttctgtt cgagatcggc gaagagctga
     7021 aggtcgaggt cgagcgcgat ggcgtcacga aggagctcgt cgcgcgtgtc gtctcgcact
     7081 cgggtcccgg cgacgccaag gtcaccgagc tggagcttgt cagcccatga tgatcggcga
     7141 tctcgacgac gttcagtacg atctcgagct cgacggtgag ctcgtccgca agcagcttgc
     7201 gcggcgcgtt tgggagcatc gcggctgggc aacggtcgcg atcgcgtttc aggagcgcga
     7261 taaggccggc gaatggaagc ccgcgaagct cgcgctactg cggtttcgcc acgagcgcga
     7321 ggcgtggaag aaacaggcag cggtgacgat cgatggcgcc gacgccctgg ccctcgcgga
     7381 gacgctcgag agctggcgag agcacctcgg ctagcggcgc caaattggcc gaacctcttc
     7441 ttctacgccg ccgctagccg ggttaacctc gctcagcgga tgctcaagcc tgtcgagaag
     7501 cagcgtgtag ccgaggagat tgcggagcag ctgcggtcgt tgatcctcaa cggccagtac
     7561 ccgcccgggt cgaagctgcc tccggagcgc gagctgtcga agcggcttcg ggtcaaccgt
     7621 gcctcgctgc gcgaagccct caagaagctc gagcatctcg ggctcgtccg catccgccag
     7681 ggtgatggca cccgcgtcca gaacttcatg gagacgggtg gcatcgagct cgtccagcac
     7741 ctgttgccgc tcggtggtgg caagccggag ctgatccgcg acctgctcga gtttcgccgc
     7801 atcttcggtc gcgagctcgc gcgtcttgca gctgcgcggt cgggcaagga tcgcgatggt
     7861 ctcgcgaagc tgcgcgcgct cgccgacaag gccgataaca cgacgagcgc cgccgatctg
     7921 ttcgatctcg atttcgactt ctacgtcgcg atcgcgagca tgtcgggcaa tcaggtcatg
     7981 ctgcttctgc tcaacacggt gcgcgacggc gtgcgcggat tcatgccgct gctcgcgaac
     8041 ctcgccggtc ccggcgacca agtgcgcaag catcaccgcg agctgatcac ggcgatcgag
     8101 aaaggcgaca tcacgagcgc cgcgcggatc gccgacgagt atttgaagat gggcgcagag
     8161 ctcgccgctc gcatgggcgg tcgccccgag ctgcagccgc agcctctgcc cgagtgatcc
     8221 gtgccggcgt tgacgcgtaa agaggtcgaa gagatcgcgc tgctcgcgcg tcttcacttg
     8281 gagcccgcgg agctgacgtc gctcgagcaa gagctcggcg cgatcctcga gcatttctcg
     8341 acgctcgcgt ccgtcgacac gacagacgtt ccgccgatga cgcatgccgt gccgctggac
     8401 ttgcggctgc gttccgacga ggtcgcgccg tcgttgacac aagccgaagc gctcgcgaat
     8461 gcgccggcga agcagggcga cgtgttcgtc gttcccgcaa tcattacgga gaaggaatga
     8521 gcgagtggct ctccgtcgcc gagaccgcgc agaagatcgc ggcgggtgag ctcactgcgg
     8581 ttcgcgtgat cgatcgctac atcgagcgca tacgcgatgt cgatcgcggt ctgaaggcgt
     8641 acctgacgct cgatgcagag cgtgcgcgtg cagaggcagt cgcgatcgac aagcgccgcg
     8701 cggcggggga gcggctgccg cggctcgcgg gcgtgcccat ctcgctcaag gacgtgctcg
     8761 tcacgcgtgg catgccgacg acagcgggct cgaagatcct cgagagcttc gtcgcgccgt
     8821 acgatgccgc ctgcgtcgag aagctgaagg ccgccggcgc gatcatgctc ggcaaggtca
     8881 actgcgacga gttcgcgatg ggatcgtcga cggagaattc ggcgtaccag cagacgaaga
     8941 atccgtggga tccgcagcgc gttcccggcg gcagctcggg cggcagctct gcggcggtcg
     9001 cggcgggcct ctgtgcggcg agcctcggca cggacacggg cggttcgatt cgccagccgg
     9061 cggcgttctg cggcgtcgtc gggctcaagc cgacgtacgg gcgggtgtcg cgctggggtg
     9121 tcgtcgcctt cgcgtcgtcg ctcgatcagg taggcccgat cacgcgcacg gtgcaagacg
     9181 cagcgcttct gctcgaggtg atcgcgggct ttgatccgcg cgattcgacg tcgctcgacg
     9241 agccagtgcc gcaatacagc gaggcactcg tcggcgatgt cacgggcctg cgcatcgggc
     9301 tgccgcacga atacttcggg gagggcatcg accccgaggt gcgcgctgcg ctcgacaaga
     9361 cgatcgcggt gctcaaagag cgcggcgcga tcactgtcga catcacgctg ccgcacacgc
     9421 atctcgcgct gcccgcgtat tacatcgtcg cgcctgcaga agcgtcgtcg aacctcgcgc
     9481 gctacgacgg cattcgcttc agcaaagcgg cgagcggcgc gaaggacctg ctcgacctct
     9541 atatgaagtc gcgcggtgca ggctttggcc gcgaggtgaa gcgccgcatc atgctcggca
     9601 cgtacgcgct gcgcagcggc tactacgacg cgtactacaa gaaggcgcag caggtccgca
     9661 cgttgatcaa gcgcgacttc gacaaggcgt tccagtactg cgacgtgatc ctcacgccga
     9721 cgacgccgac gcctgcattc aagcgcggca gcaagtcgaa cccactcgac atgtacctag
     9781 gcgatgtgtt cacgctgtcg tgcaacctcg ccggactgcc gggcctcagc gtgccgtgtg
     9841 gcctgacgag cgagaaactc cccgtcggtg cgcagctgct cggcaagccg ctcgacgaag
     9901 cgacgctgct gcgcgtcggt cacgtgatcg aatcgctgtg cggcctccgc ggccagattc
     9961 cgggcggaat gtcgtgagtg acgcgcacct ctacgagacg tacgaagccg tgatcggcct
    10021 c
//
LOCUS       DATLBZ010000608        12806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3494470, whole genome shotgun sequence.
ACCESSION   DATLBZ010000608 DATLBZ010000000
VERSION     DATLBZ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12806
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3494470"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VMZ53_29835"
     CDS             complement(<1..309)
                     /locus_tag="VMZ53_29835"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HUS32753.1"
                     /translation="MDTLETMTYEVTGRIARITLNRPQRGNGITMKMPHELAACVERA
                     NLDPTVHVIALAGNGSGFCGGYDLVESAEGFGANAKDDPRRPKGSPMDPAVIASNHDP
                     R"
     gene            complement(313..1065)
                     /locus_tag="VMZ53_29840"
     CDS             complement(313..1065)
                     /locus_tag="VMZ53_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUS32754.1"
                     /translation="MSQETLGDWLDAQPFALAMSSGFFSFFAHTGFLDAVTSRGLAPQ
                     LVAGSSAGALVGGAWAAGLDARALAERFTTLRRDEFWDPAPGAGLLAGKKFDRLLREL
                     LPITAMKELRVPARISAFDIRGRTTAVLHDTDLAHAIRASCAVPAMFHPVWIDGRAYW
                     DGGILDRPGLAGIPAGERVLFHHIASKSPWRARSPAPPTREGMVTLVIDNLPRSGPFR
                     LDAGRRALELAREATKRALDAPVIDGVVRVYT"
     gene            1209..1667
                     /locus_tag="VMZ53_29845"
     CDS             1209..1667
                     /locus_tag="VMZ53_29845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32755.1"
                     /translation="MKHLAFIVAVSALAVGCVAETETGDDDGTGDMMEGSGSGSGSDS
                     QNVTCNASATVSPVVSASRIDAVGKATCSATPSSVTVETCIQWKSSSSTSFTDMGCAS
                     ASKSGVATLEEPFTFGCGISTAGKLYRTHAKVKLNGTVVAEASSNEVTCY"
     gene            complement(1664..2035)
                     /locus_tag="VMZ53_29850"
     CDS             complement(1664..2035)
                     /locus_tag="VMZ53_29850"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS32756.1"
                     /translation="MSDRDPAQLRAIAETWLACFERRDLDGLLALYADDATHTSPKIR
                     VRHPETGGLLRGKAAMREWWADSFARLPSMQYVPVAITADRERVYYEYVRKVEGEADM
                     PVCEVLDVRDGMIVASRVFHG"
     gene            2086..2421
                     /locus_tag="VMZ53_29855"
     CDS             2086..2421
                     /locus_tag="VMZ53_29855"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUS32757.1"
                     /translation="MLTLDDLDDSALDQLPFGVVCLDEHLKVVRMNKTESDASGIQRW
                     RAMGREFFRDVAPGPTNRELADAVRAFADGSGPRAVSHVFHRRAGDDATQIELVHGRD
                     AAVYLCIRR"
     gene            complement(2418..2870)
                     /locus_tag="VMZ53_29860"
     CDS             complement(2418..2870)
                     /locus_tag="VMZ53_29860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HUS32758.1"
                     /translation="MRGTIRLVAILCVALGGTAFAGHGHGGGKHGGGGRGRVVVRDHR
                     SGPSTVVVRDHRAPRVKYVRASKGRYVFPGGVVRVYKKPRIERYYDVHVRPAVIVESY
                     DPVPGYVWVSGGWTWGGSEWVWTPGYWAVADEPVVTGGVSISAGISIH"
     gene            2947..3708
                     /locus_tag="VMZ53_29865"
     CDS             2947..3708
                     /locus_tag="VMZ53_29865"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HUS32759.1"
                     /translation="MAVEVLGLLLASAGASFALRAWQRRREPRRLEEVLAEDAAVVVS
                     VATHEAGTRQHPELWPIHLLYGLLQDEPFVDAIKKLGGDPDAIESRVLAELDRRPARQ
                     HDDGAHMEAVRVLAMAHHGGERKMSAKDLWAYLSRTTTGQLVESEGLTRHALVFAFAH
                     GMPEPSTEMPGRIDVHVVLRNDDYTTREIVVDILRDVFDIPDAEAETRMMQVHTEGRG
                     VVGRFKLAVAKQKVDEARRRARERMFPLWIGLEDC"
     gene            complement(3705..4898)
                     /locus_tag="VMZ53_29870"
     CDS             complement(3705..4898)
                     /locus_tag="VMZ53_29870"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HUS32760.1"
                     /translation="MGAVRRTSPADVLRNDRKFLGRTEEPEALEIVAAEGSRVRDARG
                     RTFIDFQMGWCVGNLGWNPPEIVARIQRFRGPYYVSPKAVYAPWTRLAEGLVDIAPGK
                     LEYAYRCVGGSEAVELALQLAFTATGRSKIISLEGAYHGNTFGARSVGEGDLDAKLAG
                     MKKLAPPLDADALERLETLLKHNDVAALIMEPISMNLNVLVPESDFMRELIPLCHRYG
                     ALVIFDEVACGFGRTGKLFAAEHWHAEPDIMCVAKALSSGIAPIASTLATADVADAAK
                     GELSFYSTFGWMPLATEAALGTLAYWRTKGDSLLENIAERSTQLRHGLTEIFDDAELR
                     IHGLAVAVGLDDKERAARITKRCKDHGLLLGEEEDFLLMWPALTVDEPTAAKALEIIE
                     TAARR"
     gene            5030..6322
                     /locus_tag="VMZ53_29875"
     CDS             5030..6322
                     /locus_tag="VMZ53_29875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32761.1"
                     /translation="MSKRILLGLTLLAACAGDDPGIPEEAPTAPSKEDSPGYTVTAKH
                     WLLVGDSLTPGDAKFTATVKAPADVSTVDLWIDGHYVSSAQGHGTFTLTANAEDLAIG
                     DHVALLAKRGSTQAFASVKITRTAPLYVAVSNDWDDPDHTDDKLERQERLHTRHPHMV
                     ITHFVGPYTFTDPSVSAARQQVLVDWLKGYEAKGDEIGVHIHPWCNFVQAAGVTCRTS
                     PSFAHASDTTGYTVILGSYTEPELEKLFKKANELFAAHGLGRPTSFRAGGWTATSDVI
                     TAMGKTGYKVDASAANWARLEEWRGVANASIYEWNQQHWGPVDETTQPYYPSVDDILA
                     DAAPHQRVLEVPDNGALVDYVSAQEMIDMFRVNFDGRALTEAKQYSIGYHPVDFSESF
                     FQRIDGALTEIDKHLAVDDRGPVIYARMTDLVKAFPAP"
     gene            complement(6273..6758)
                     /locus_tag="VMZ53_29880"
     CDS             complement(6273..6758)
                     /locus_tag="VMZ53_29880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32762.1"
                     /translation="MKKLMLGVVAAGAVGYGVYHWRAAAPLHAQTELVENRLWIDHMP
                     RTERDTIQVFLTLGDEGMGTFQQTSAWKGAYEIFQYEGHAGELRIIYPQTGDRETVHA
                     KATKCRENGFDYCLEMNGGTRGVKRYYSMKDWEIGSMRELDSKVKALGTPSPDPSSAR
                     K"
     gene            7001..7963
                     /locus_tag="VMZ53_29885"
     CDS             7001..7963
                     /locus_tag="VMZ53_29885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myxococcus cysteine-rich repeat containing
                     protein"
                     /protein_id="HUS32763.1"
                     /translation="MRFVSTVPFVLAASLFGGCVLGESQQSDELTSIARLSGAIFTTL
                     PDGSRVNANLYPSKDDVYLDGGPGNQAPASAAALPEGDYYFQVTDPSGKVLLSLDAIQ
                     CRELHVDAAGLISAVGAAGCSHVTGIDVDHGALTVQLMPYADTPNPGGEYKVWITRVE
                     DYDIRAKRFHGFVPSESKTDNFKVRVPELPPPPPPCEGSGCNPPPPPPCDGSCTPPPP
                     PTSCVCGDGHLDAGEGCDDGNLVNGDGCSSTCTKESTSTCGGTCPTTPPSTEPGGDAG
                     TGSTGEGGSGSSAPTPTPEPAHDRVFDHADQINDAYDFDGGVLL"
     gene            complement(7970..8356)
                     /locus_tag="VMZ53_29890"
     CDS             complement(7970..8356)
                     /locus_tag="VMZ53_29890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32764.1"
                     /translation="MRYPFLSDEWFTEVDKLIAAAGDLQIPDAMKNAKVNITVTTSSG
                     STQLSMKDGLFTRGHDPSFTTTLTVADDIARKIFVEGDAAAGVQAFLEGKIQVDGDLA
                     TVVAMQTVEPSEPQKKLTRQIAAITA"
     gene            8387..8713
                     /locus_tag="VMZ53_29895"
     CDS             8387..8713
                     /locus_tag="VMZ53_29895"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HUS32765.1"
                     /translation="MKCPGCTSDMVTTDVHSIEIDRCPNCGGIVLDKGEQDTIEGLGL
                     GAAVEGGVQQAHEHRNTTAHCYECRRDMIALKGAGDVEYDWCDGCERLFFDRGELTAI
                     DAFVDS"
     gene            8753..9766
                     /locus_tag="VMZ53_29900"
     CDS             8753..9766
                     /locus_tag="VMZ53_29900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32766.1"
                     /translation="MKLIWRACTGVLLALAVAACPPPSQNTGGGGGGGGGGAGISPDT
                     CGKIDSSNVGRKVYAFLVASSELDRASFELENSVKNACVKMARELGVSPEGDTKTVCM
                     RAKAELDANLQVSIKTEKRFVTKYTPPVCKTDVDFTASFAAQCEAKASADISVQCSGR
                     CGGTCNGACDGTCSSGGAGGQCNGQCNGTCRGSCSGSCEGYADVKASAECKASAEIRA
                     TTHTTCTPAKTEIVQQDVTVVDATKWNKAMAAINVGLPELLTAKAKLEMTAKALVLWV
                     QTGASLAKSIGSFADQLGEHTVCVGAQLAGVVAAAANIEARINVSIEVSASVSASAGA
                     TAQ"
     gene            10173..11075
                     /gene="ligD"
                     /locus_tag="VMZ53_29905"
     CDS             10173..11075
                     /gene="ligD"
                     /locus_tag="VMZ53_29905"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HUS32767.1"
                     /translation="MASKLKIEADGHTIECTNTDRVVFPDVGVTKGDVIEYYSDLADI
                     MVPEMEGRPLTLERFTKGLAGGGFFQKHVQKHFPPWIDRVTLGAKTVVTYPMANSAAA
                     LVYFANQGALAFHVWTSREATPTQPDIIVFDLDPPEGGFELVRQTALILRDALKEIGL
                     VGFVKTTGSKGLHVVAALDGKDDFDDVSTLGQELGKQLALKHPKLVTMEFYKKDRGGR
                     LYFDVMRNTGGATFVAAYSLRGKANAPVSTPLEWDELADKKLRSDMHTLQTVRERLAK
                     NGDPWKSLRKKPGSATKALARLRG"
     gene            11091..12482
                     /locus_tag="VMZ53_29910"
     CDS             11091..12482
                     /locus_tag="VMZ53_29910"
                     /inference="COORDINATES: protein motif:HMM:NF017396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070008 - serine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HUS32768.1"
                     /translation="MRRAALLVSLLAACGDDLGPDGDVLEQLKDLPGVTAVEWFPPAD
                     FGAEEGYRFFDVTFTQPIDHDNPGGPTFQQYAAFMVRDPSAPLVFYTSGYGASWKRRL
                     SEPADILDANQLSLEYRFYERSRPSADWSKLTVKQASADEHAILEAIDPIFNGKTIQT
                     GGSKGGENTMFHLWLYPDDLDGAVAYVPPVITEWLDPRYATNLDDIGANLPACRDALR
                     AVQREMLIRRTAMESFASETADFTIAGVAHATETAIVELEFSFWMTRGEHDCPLVPPT
                     TATDEELFTFLNETGGPYAYGDQDLYLYGNQYIFQDHNELGYPVWQHAHLDDLLQFSY
                     EDWSAYLPTEKPAYDPTMPRGLAAWLATSPPHLMIVGGEWDPWGPGYPAVTADADTRV
                     YSVPHASHWTASIFALPPAEKDEALATLAQWGGVTTHRSRRPQFPMGFTPSRRLIETT
                     PITQTPLRNVVGE"
     gene            complement(12449..>12806)
                     /locus_tag="VMZ53_29915"
     CDS             complement(12449..>12806)
                     /locus_tag="VMZ53_29915"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS32769.1"
                     /translation="LAGVGGEIAAAIGSNAGAAEARLDRLVARVVEALRDPAAELPRL
                     SISDAHLQELFARDVGVPMRTFRLWHRLLRTLVLFTTRDATSAAHEAGFADLAHFSRT
                     CRRFLGYSPTTLRNGV"
BASE COUNT         2291 a         4246 c         4151 g         2118 t
ORIGIN      
        1 tcgcggatcg tgattgctcg cgatcaccgc cggatccatc ggcgagcctt tcggccgtcg
       61 cggatcgtct ttcgcgttcg cgccaaagcc ttcggcgctc tcgacgaggt cgtagccgcc
      121 gcagaagccg ctgccgtttc cagcgagcgc gatgacgtgc acggtcggat cgaggttcgc
      181 ccgctcgacg catgccgcga gctcgtgcgg catcttcatc gtgatgccat tgccgcgttg
      241 cggccgattg agcgtgatgc gcgcgatccg tccggtgacc tcgtaggtca tggtttcgag
      301 ggtgtccacg gcctacgtat acacccgcac caccccgtcg atgactggcg catccaacgc
      361 ccgttttgtc gcttcacgcg ctagctcgag tgcgcgccgt ccagcatcga ggcgaaacgg
      421 tcccgagcgc ggcaagttgt cgatgacaag cgtgaccatg ccttcgcgcg tcggcggcgc
      481 tggagacctc gcacgccacg gcgacttcga tgcgatgtga tgaaacagca cgcgctcacc
      541 ggcgggaatt cccgcgaggc ccggtcgatc gaggatgccg ccgtcccagt acgcgcggcc
      601 gtcgatccac acgggatgaa acatcgcggg caccgcacac gacgcgcgaa tcgcatgcgc
      661 gagatccgta tcgtgcagca ccgccgtcgt gcggccgcga atgtcgaacg cgctgatgcg
      721 cgcaggcacg cgcagctcct tcatcgcggt gatcggcaac agctcgcgca gcaggcgatc
      781 gaacttcttg ccagcgagga gcccggcccc cggcgccgga tcccagaact cgtcgcgacg
      841 cagcgtcgtg aaccgctccg cgagcgcgcg cgcatcgagg cccgccgccc acgcaccgcc
      901 gacgagcgcg cctgcactcg agcccgcgac gagctgcggc gcgagcccac gtgacgtcac
      961 cgcatcgaga aatccagtgt gcgcgaagaa cgagaagaag ccgctcgaca tcgcgagcgc
     1021 gaacggctgc gcatcgagcc agtcgccgag cgtttcttgt gacatcggtg tgaccatttc
     1081 atgccagatg cggagcgtct cgtgccatgg ggaggctgca cgacaagcca cggcggttcc
     1141 gcgagccacg acgagccgcg gcagatcaaa cacttgagct ggcacgcacc gtggagttgc
     1201 acggcagcat gaagcacctc gcattcatcg tggccgtatc ggcgttggca gttggttgcg
     1261 tcgcggagac cgaaaccggc gatgacgacg ggaccggcga catgatggag ggatcgggct
     1321 ccggctctgg ctccgactcg cagaatgtga cgtgcaacgc gagcgcgacg gtgtcgccgg
     1381 tcgtatcggc atcgcgcatc gatgccgtag gcaaggcgac gtgcagcgcg actccgagca
     1441 gcgtcaccgt cgagacgtgc atccaatgga agagctcgtc gtcgacgagc ttcacggaca
     1501 tgggctgcgc atccgcatcg aagagcggcg tcgcgacgct cgaggagccg ttcacgttcg
     1561 gctgcggcat cagcacagcc ggcaagctct accgcaccca cgccaaggtg aagctcaacg
     1621 gcaccgtcgt cgccgaagca tcgtcgaacg aagtgacctg ctattagccg tggaacacgc
     1681 gactcgcgac gatcatgccg tcgcgcacgt cgagcacttc gcagaccggc atgtcggcct
     1741 cgccttcgac cttgcgcacg tattcgtaat agacgcgctc gcgatcggcg gtgatcgcga
     1801 ctggcacgta ctgcatcgac ggtagccgcg cgaacgagtc cgcccaccac tcgcgcattg
     1861 cggccttgcc tcgcagcaat ccgccggtct ccggatggcg cacgcggatc ttcggcgacg
     1921 tgtgcgtggc gtcgtcggcg tagagcgcga gcaggccgtc gagatcgcga cgctcgaagc
     1981 acgcgagcca ggtctctgcg atcgcgcgca gttgcgcggg gtctcgatct gacatgcgca
     2041 ggagtgtaac gtcgggccgc cttcgcggct ccaatgggta tgcagatgct gacgctagat
     2101 gatctcgacg attcggcgct cgaccagctg ccgttcgggg tcgtctgcct cgacgagcac
     2161 ctcaaagtcg tgcgcatgaa caagaccgag tcggacgcca gcggtattca gcgatggcga
     2221 gcgatgggcc gcgagttctt ccgcgacgtc gcgccggggc cgacaaatcg cgagctcgcg
     2281 gatgccgttc gcgcgtttgc cgatggcagc gggcctcggg cggtcagcca cgtgtttcac
     2341 cgccgggccg gcgacgacgc tacgcaaatc gagctcgtgc acggccgcga tgcggcggtc
     2401 tatctctgca tccgccgtta gtggatcgag atgccagcgc tgatgctcac gccgcccgtg
     2461 acgacgggct cgtcggcgac agcccaatag ccgggcgtcc agacccactc gctgccgccc
     2521 cacgtccagc cgccggagac ccagacatag cctggcaccg gatcgtagga ctcgacgatg
     2581 acggcgggac ggacgtggac gtcgtagtag cgctcgatcc ggggcttctt gtagacgcgc
     2641 acgacgccgc ccgggaatac gtaacggccc ttcgaagcgc ggacgtactt cacgcgcggt
     2701 gcgcggtgat cgcgaacgac gaccgtgctc gggccgctgc ggtgatcgcg aacgacgact
     2761 cgcccgcgac cgccaccacc atgcttgcct ccgccgtggc cgtgacccgc gaatgcagtg
     2821 ccaccgagtg caacgcagag gattgcgaca agtcgaattg tgcctctcat ggtgcacagg
     2881 ctaacgcggg gtagatgtcg gcatggcacc gcaatgcatg tacaagtttt aaccgtaaga
     2941 tagagcgtgg ccgttgaagt cctcgggctc ttgctagcca gcgccggtgc aagcttcgca
     3001 ctgcgcgcat ggcagcggcg tcgcgagcca cgtcgcctcg aagaggtgct cgcggaggat
     3061 gcggccgtcg tcgtctcggt cgcgacgcat gaagctggca cgcgccagca tccagagctc
     3121 tggccgattc atctgctgta cgggctcttg caagacgaac cgttcgtcga cgcgatcaag
     3181 aagctcggcg gcgacccaga cgcgatcgag agccgggtgc tcgcggagct cgatcgcagg
     3241 ccagcgcgtc agcacgatga cggcgcgcac atggaagccg tccgcgtgct cgcgatggcg
     3301 caccacggcg gcgagcgaaa gatgtcggcg aaagatctgt gggcctatct atcgcgcacg
     3361 acaaccgggc agctcgtcga gagtgaagga ctcacgcgtc acgcactcgt gttcgcgttc
     3421 gcgcatggca tgcccgagcc gagcaccgag atgccaggcc gcatcgatgt tcacgtcgtc
     3481 ctgcgcaacg acgactacac gacgcgcgag atcgtcgtcg atatcctgcg cgacgtcttc
     3541 gacattcccg acgccgaagc cgagacccgc atgatgcagg tccacaccga gggccgcggc
     3601 gtcgtcgggc ggttcaagct cgccgtcgcc aagcagaagg tcgacgaagc tcgtcggcga
     3661 gcgcgcgagc gcatgttccc gctctggatc ggcctcgaag actgctagcg acgagcggcc
     3721 gtctcgatga tctcgagcgc tttggcggcg gtcggttcgt cgacggtgag cgcgggccac
     3781 atcaggagga agtcttcctc ttcgccgagg agcagcccgt gatctttgca gcgcttcgtg
     3841 atgcgcgcgg cgcgctcctt gtcgtcgagg ccgactgcaa ccgcgagccc gtgaatgcga
     3901 agctctgcgt cgtcgaagat ctcggtgagc ccgtgacgca gctgcgtaga ccgctccgcg
     3961 atgttctcga ggaggctatc gcccttcgtg cgccaatacg cgagcgtgcc gagcgcagcc
     4021 tcggtcgcga gtggcatcca gccgaacgtc gagtagaacg acagctcacc tttcgccgcg
     4081 tcggcgacgt ccgcggttgc gagcgtcgat gcgatcggcg cgatgccact cgatagcgcc
     4141 tttgcgacgc acatgatgtc gggctcggcg tgccagtgct ccgccgcgaa cagcttgccc
     4201 gtgcgaccga agccgcacgc gacctcgtcg aagatcacga gcgcgccgta gcggtgacac
     4261 agcgggatga gctcgcgcat gaagtcgctc tcaggcacga gcacgttcaa gttcatgctg
     4321 atcggctcca tgatcagcgc agcgacatcg ttgtgcttca gcagcgtctc gagacgttcg
     4381 agtgcatccg catcgagcgg cggcgcgagc ttcttcatgc ctgcgagctt cgcgtcgaga
     4441 tcgccctcgc cgacgctgcg cgctccgaac gtgttgccgt gataggcgcc ttcgagcgag
     4501 atgatcttgc tgcgtccagt cgcggtgaat gcgagctgca aggcgagctc gactgcttcg
     4561 ctgccgccga cacagcgata ggcgtactcg agcttgccgg gcgcgatatc gacgaggcct
     4621 tccgcgagcc gcgtccacgg cgcgtagacc gcttttggcg agacgtagta cgggccgcgg
     4681 aatcgctgaa tgcgtgcgac gatctctggc ggattccatc cgagatttcc gacgcaccaa
     4741 cccatctgaa aatcgatgaa cgtgcgccct cgcgcatcgc gaacacggct gccctcagcg
     4801 gccacgatct cgagcgcctc gggctcctcg gtgcggccta gaaatttgcg atcgttgcga
     4861 aggacgtccg caggtgacgt acgacgaaca gctcccatgc actcgggagt tgcaagagtg
     4921 caaccagctg tctgcggcaa cttgcgatgc atggctactc gagttgcctg cgtaaccatc
     4981 cgagatcacg cactgcgcta cgggcactga tcttgatgac accgcgtgca tgtcgaagcg
     5041 aatcctcctc gggctgacgt tgcttgccgc ctgcgccggc gacgacccgg gaatccccga
     5101 ggaagcgccg accgcgccga gcaaggagga ctcgcccggc tacacggtga ccgcgaagca
     5161 ctggctcctc gtcggcgact cgctgacgcc cggcgacgcg aaattcacgg ccaccgtcaa
     5221 ggcgccggcc gacgtgtcga cggtggatct ctggatcgac ggccactacg tctccagcgc
     5281 gcaaggtcac ggcacgttca cgctcaccgc gaacgcagag gatctcgcca tcggcgatca
     5341 cgtggctcta ctcgcgaagc gcggcagcac gcaggcattt gcgtcggtga agatcacgcg
     5401 caccgcgccg ctctacgtcg ccgtgtccaa cgactgggac gatccagacc acaccgacga
     5461 caagctcgag cgccaggagc gcctgcacac gcgtcacccg cacatggtca tcacgcactt
     5521 cgtcggtccg tacacgttca ccgatccatc ggtcagcgcg gctcgccagc aggtgctcgt
     5581 cgattggctc aagggctacg aagcgaaggg cgacgagatc ggagtgcaca tccatccgtg
     5641 gtgcaacttc gtgcaagccg cgggcgtcac gtgccgaacc tcgccatcgt tcgcgcacgc
     5701 cagcgacacg acgggctaca cggtgatcct cggctcgtac accgagcccg agctcgagaa
     5761 gctgttcaag aaagcgaacg agctgttcgc cgcgcacggc ctcggcaggc ccacctcgtt
     5821 ccgcgccggc ggctggaccg cgacgagcga cgtgatcacg gcgatgggca agacgggcta
     5881 caaagtcgat gcgagcgcgg cgaactgggc gcgtctcgaa gagtggcgtg gcgtcgcgaa
     5941 cgcgtcgatc tacgagtgga accagcagca ctggggcccg gtcgacgaga ccacgcagcc
     6001 gtactacccg agtgtggacg acatcctcgc cgacgcagcg ccgcaccagc gcgtgctcga
     6061 ggtgccagac aacggtgcac tcgtcgatta cgtgagcgcg caggaaatga tcgacatgtt
     6121 ccgcgtcaac ttcgacggac gcgcgctgac cgaagcgaag cagtactcga tcggctacca
     6181 ccccgtcgac ttcagcgagt cattcttcca gcgcatcgac ggcgcgctta ccgagatcga
     6241 caagcatctc gccgtcgacg atcgcggccc ggttatttac gcgcggatga cggatctggt
     6301 gaaggcgttc ccagcgcctt gaccttgctg tcgagctcgc gcatcgagcc aatctcccag
     6361 tccttcatcg agtagtagcg cttcacgccg cgcgtgccgc cgttcatctc gaggcaatag
     6421 tcgaagccgt tctcgcggca cttcgtcgcc ttcgcatgaa cggtctcgcg gtcgccggtc
     6481 tgtggataga tgatgcgcag ctcgcccgcg tggccctcgt actgaaagat ctcgtacgcg
     6541 cctttccagg ccgacgtctg ctggaacgtg cccatgcctt catcaccgag cgtcaagaag
     6601 acctggatcg tgtcgcgctc cgtgcgcggc atgtgatcga tccagagccg gttctctacg
     6661 agctccgttt gcgcgtgcaa cggcgcagcc gcgcgccagt gatacacccc gtaaccaaca
     6721 gcgccggccg ccaccactcc gagcatgagc ttcttcatgc gctggctata gcgcaggccg
     6781 ggcgccgtct ccacacgagc actcctgtaa tgcgcgccct gcccaccgtc agccacgttt
     6841 cccgcgcgct tgcttgccac ggtgatcgct aactcgacgg gcgctcgttg gtgttctgcg
     6901 cgccgacgag acacttctcg ctgcggggag ttcgtaagcc ccggcaccga gcttgctgtg
     6961 gaagcgctca gtcacccacc caaggcagga gacctccaag atgcgcttcg tatccaccgt
     7021 cccgttcgtg cttgccgcca gcctgttcgg cggttgtgtt ctcggcgaga gccagcagag
     7081 cgacgagctc acctcgatcg cgcgtctctc cggtgcgatc ttcacgacgc taccggacgg
     7141 ttcgcgcgtc aacgccaacc tctatccgtc gaaggatgac gtctacctcg acggtgggcc
     7201 gggcaaccaa gcgcccgcgt cggcagcggc gctgcccgaa ggtgactact acttccaagt
     7261 caccgacccg agcggcaagg tcctcctctc gctcgatgcg atccagtgcc gcgagctcca
     7321 tgtcgacgcc gctggcctca tctcggcggt cggcgccgcc ggctgcagcc acgtgaccgg
     7381 catcgatgtc gatcacggcg cgctgaccgt gcagctcatg ccgtacgcgg acacgccgaa
     7441 ccccggtggc gagtacaagg tgtggatcac gcgcgtcgag gactacgata ttcgcgcgaa
     7501 gcgcttccac ggcttcgtgc cgtcggagag caagaccgac aacttcaagg ttcgcgtccc
     7561 cgagcttccg cctcctccgc cgccgtgcga aggctccggc tgcaatccgc cgccgccgcc
     7621 gccgtgcgat ggcagctgca cgcctccgcc tccgccaacg agctgcgtgt gtggtgacgg
     7681 ccacctcgac gccggcgaag gctgcgacga cggcaacctc gtgaatggcg acggctgctc
     7741 ttcgacgtgc acgaaggaat cgacgtcgac gtgcggcggc acctgcccga cgacgcctcc
     7801 gtcgacggag ccaggcggtg atgctggtac tggctcgacg ggtgaaggcg gcagcggctc
     7861 gagcgcgccg acgccgacgc cggaaccggc ccacgaccgc gtgttcgacc acgccgatca
     7921 gatcaacgac gcctacgact tcgacggcgg cgtcctcctc taacgatcgc tacgcggtaa
     7981 tcgccgcgat ttgccgagtg agcttctttt gcggctcact cggctcgact gtttgcatcg
     8041 caacgaccgt ggcgagatcg ccgtcgactt ggatcttgcc ctcgaggaat gcttgaacgc
     8101 cggcagcggc atcgccctcg acgaaaatct tccgcgcgat gtcgtcggca acggtgagcg
     8161 tcgtcgtgaa tgatggatcg tgaccgcgcg tgaacaggcc gtccttcatc gagagctgcg
     8221 tgcttccgct ggacgtcgtc accgtgatgt tgaccttggc gttcttcatc gcgtcgggaa
     8281 tctggaggtc acctgcggca gcgatcagct tgtcgacctc ggtgaaccat tcgtcggaca
     8341 ggaacgggta acgcatgcca caccataact ggagtatcgt gcccgcatga agtgccccgg
     8401 gtgcacgagc gacatggtga cgacggacgt tcactcgatc gagatcgatc gctgtcccaa
     8461 ctgcggtgga atcgtcctcg ataaaggcga gcaagacacg atcgaagggc tcggcctcgg
     8521 tgcagcggtc gaaggcggcg tgcagcaagc gcacgagcat cgcaacacga cggcgcattg
     8581 ctacgaatgc cggcgcgaca tgatcgcgct caagggcgca ggcgatgtcg aatacgattg
     8641 gtgcgacggt tgcgagcgcc tgttcttcga ccgcggcgag ctgaccgcga tcgatgcttt
     8701 cgtcgatagc tgacttgcta tcgccctagc gctcgcagca acatccgccg ctatgaagct
     8761 catctggcgt gcgtgtacgg gtgtcctgct ggcgctggcg gtcgcggcgt gtccgcctcc
     8821 atcgcagaat acgggcggcg gtggtggtgg cggtggcggc ggcgcaggca tcagccccga
     8881 cacgtgcggc aagatcgaca gctcgaacgt cggccgcaag gtctacgcgt tcctcgtcgc
     8941 gtcctcggag ctggatcgcg cgagcttcga gctcgagaac agcgtcaaga acgcgtgcgt
     9001 gaagatggcg cgcgagctcg gtgtctcgcc ggaaggcgac acgaagaccg tctgcatgcg
     9061 cgcgaaggct gagctcgacg cgaatctcca ggtctcgatc aagaccgaga agcgcttcgt
     9121 cacgaagtac acgccgcccg tgtgcaagac ggacgtcgac ttcacggcga gcttcgcggc
     9181 gcagtgcgag gcgaaggcct cggcggacat cagcgtgcaa tgctccggtc gctgcggcgg
     9241 cacgtgcaac ggcgcgtgcg atggcacctg ctcgtcgggc ggcgctggcg gccagtgcaa
     9301 cggtcagtgc aatggcacgt gccgcggctc gtgcagcggt agctgcgaag gctacgcgga
     9361 cgtgaaagcg tcggcggagt gcaaagcgtc tgcagaaatc cgcgcgacga cgcacacgac
     9421 gtgcacgccg gcgaagacgg agatcgtgca gcaggacgtc accgtcgtcg acgcgacgaa
     9481 gtggaacaag gcgatggcgg cgatcaacgt cggcctcccc gagctgctca cggcaaaggc
     9541 gaagctcgag atgaccgcga aggccctcgt gctctgggtg cagaccggcg cgtcgcttgc
     9601 gaagtcgatc ggctcgttcg ccgatcagct cggcgagcac acggtgtgcg tcggtgcgca
     9661 gctcgcaggt gtcgtcgcgg ccgccgcgaa catcgaggcg cgcatcaacg tgtcgatcga
     9721 agtgtcggcg tcggtgtcgg catccgccgg cgcgaccgcg cagtaaaacc tagacctcgg
     9781 cctgaacctc aacctcgacg tcaaacctcg acgttgaggt gaaccggtcc acctcgactg
     9841 acgtaacgct ccggcctgtg attgaacagg gagacacgga gcacacgaag ccgagaaggg
     9901 atcgaaccga tccgccgatc cgggtccgcc gatgccgatc caggtccgcc gatccgggcc
     9961 cgccgatccg ggtccgccga tccgggcccg ccgatccggg tccgccgatc cgtgtgcgcc
    10021 gatgcgccat ccgccaatcc gcccattctg tccttcgtgt ctccgtgtct ccctgttgat
    10081 ccgtcccggg ccgacggtgg ccgacggtga ccggtcaaag tcgacgagga cggtagcgtt
    10141 caggttcggg ttcaggtcaa ggtcgaggtc tcttggcgtc gaagctcaag atcgaagccg
    10201 acggccacac gatcgagtgc acgaacaccg atcgtgtggt cttccctgac gtcggcgtca
    10261 cgaagggtga cgtgatcgag tactacagcg acctcgccga catcatggtg ccggagatgg
    10321 aaggtcgccc gctcacgctc gagcgcttca cgaaagggct ggccggcggc ggcttctttc
    10381 agaagcacgt gcagaagcac ttcccgccgt ggatcgaccg cgtgacgctc ggcgcgaaga
    10441 cggtcgtgac gtatccgatg gcgaattcgg cggcggcgct cgtctacttc gcgaatcaag
    10501 gcgcgctcgc gtttcacgtg tggacgagcc gcgaggcgac gccgacgcag ccggacatca
    10561 tcgtcttcga tctcgatccg ccagaaggtg gcttcgagct cgtgcggcag accgcgctca
    10621 tcctgcgcga cgcgctcaaa gagatcgggc tcgtcggatt cgtgaagaca acggggagca
    10681 aggggctgca tgtcgtcgcg gcgctcgacg gcaaagatga cttcgacgat gtttcgacgc
    10741 tcggtcagga gctcggcaag cagctcgcac tgaagcatcc aaagctcgtc acgatggagt
    10801 tctacaagaa ggatcgcggc ggccggctgt acttcgatgt gatgcgcaac acgggcggcg
    10861 caacgttcgt cgctgcgtac tcgctgcgcg gcaaggcgaa cgcgccggtg tcgacgccgc
    10921 tcgaatggga tgagctcgcc gacaaaaagc tgcgttctga catgcacacg ctgcagaccg
    10981 tgcgcgagcg cctggcgaag aatggcgacc cgtggaagtc actgcgcaaa aagcccggca
    11041 gtgcgacaaa ggcactcgcc aggcttcgcg gctagcggca tagtcgagag atgcgccgcg
    11101 ctgccctgct cgtttcactc cttgcagcgt gcggcgatga tctcgggccc gacggcgacg
    11161 tcctcgaaca gctgaaggat ctgcccggcg tgacggcagt cgagtggttc ccgcccgccg
    11221 acttcggcgc ggaagagggc tatcgcttct tcgacgtcac gttcacgcag ccgatcgatc
    11281 acgacaatcc cggcgggccg acgtttcaac agtacgccgc attcatggtt cgcgatccga
    11341 gcgcgccact cgtgttctac acgtcgggct acggcgcgtc gtggaagcgt cggctgtccg
    11401 agccggctga catcctcgac gcgaatcagc tgagcctcga gtaccgcttc tacgagaggt
    11461 cgcggccctc ggccgattgg tcgaagctga cggtgaagca agcatccgcc gacgagcacg
    11521 cgatcctgga agcgatcgac ccgatcttca acggcaagac gatccagacc ggcggcagca
    11581 agggtggcga gaacacgatg ttccacctct ggctgtatcc ggatgatctc gacggcgccg
    11641 tcgcgtacgt cccgccggtg atcaccgagt ggctcgatcc gcgctacgcg acgaacctcg
    11701 acgacatcgg cgcgaatctg ccggcgtgcc gcgatgcgct gcgcgcggtg cagcgcgaga
    11761 tgctgattcg ccgcacggca atggagagct tcgcgtccga gaccgcagac ttcacgatcg
    11821 ctggagttgc gcatgcgacc gagactgcga tcgtcgagct cgaattctcg ttctggatga
    11881 cgcgtggcga gcacgactgc ccgctcgtgc ctccgacgac cgcgaccgac gaagagctgt
    11941 tcacgttctt gaacgagacc ggcggcccgt acgcgtacgg cgatcaggat ctctatctct
    12001 acggcaatca gtacatcttc caagaccaca acgagctcgg ctatccggtg tggcagcacg
    12061 cgcacctcga cgacctgctc cagttctcct acgaggactg gtcggcgtac ttgccgacgg
    12121 agaagcccgc gtacgatccg acgatgccgc gcggcctcgc tgcatggctc gcgacgtcgc
    12181 cgccgcatct catgatcgtg ggtggcgagt gggatccgtg gggccccggt tatcccgcgg
    12241 tgacggccga tgccgatacg cgcgtctact cggtgccgca cgcgagtcac tggaccgcga
    12301 gcatctttgc gttgccgccc gccgagaagg acgaagcact cgcgacgctc gcgcaatggg
    12361 gcggggtcac gacgcatcgc tcgcgccgtc cgcaattccc gatgggcttc acgccgtcgc
    12421 gccggctgat cgagacaacg ccgatcactc aaacgccgtt gcgcaacgtc gtcggcgagt
    12481 agccgagaaa tcgccggcac gtccgcgaga agtgcgcgag gtccgcgaag cccgcttcgt
    12541 gcgcagcact tgtcgcgtcg cgcgtcgtga ataacacgag cgtgcgcagc agccggtgcc
    12601 acaggcggaa cgtgcgcatc ggtacgccga cgtcgcgcgc gaacagctcc tgcaggtgcg
    12661 cgtccgagat agacagccgc ggcagctccg ccgccggatc gcgcagcgct tcgacgactc
    12721 gcgcaaccag gcgatcaagc cgcgcctcag ctgcacccgc attcgacccg atcgccgccg
    12781 cgatctcgcc gccgacgccc gccagg
//
LOCUS       DATLBZ010000609         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7882079, whole genome shotgun sequence.
ACCESSION   DATLBZ010000609 DATLBZ010000000
VERSION     DATLBZ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7882079"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VMZ53_29920"
     CDS             <1..495
                     /locus_tag="VMZ53_29920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32770.1"
                     /translation="VKPRSSQKPTKVAAKAPVTAAPIVAAATPSEAAPIEAAPVPLVA
                     PVAVGAPESAPPLPLEVVIGDDTAPLLALDQKPMTEAELVVEYQRVGRDIAKLQKDRG
                     TDKVLDLWPTFRSIKLKESCKTAETRLNLEGTLKQLRSHIDRRRGITISADCMNNPLA
                     ANCQ"
     gene            complement(506..1228)
                     /locus_tag="VMZ53_29925"
     CDS             complement(506..1228)
                     /locus_tag="VMZ53_29925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32771.1"
                     /translation="MKLGTFIVAALCAAASTNAFAGTHDRAIEHYREQYEQRSDPHLL
                     TLIADEYRAAGQMREALDYYCSYMYVDAAGEDADRASLGARELAVRLGHSGATDHEAC
                     NPKKGEVAKPVTGVEALDLRIPPPAARITKREVVGLSMLAAGIGGLGMALYEGREVAR
                     VRGAQIVNDPTVNVEDLAAQADAHQRNQKLWLLAGGATMITGGILYVVGRHDRKQQDA
                     RALVIAPTANKNGGGLVMGRRF"
     gene            1327..>2534
                     /locus_tag="VMZ53_29930"
     CDS             1327..>2534
                     /locus_tag="VMZ53_29930"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HUS32772.1"
                     /translation="MGRFTQFFLVVGLVACGDDAQTPQDAATPDASAALVLEPASFDF
                     GDVEVGVPSTATHNFVVRNTSTAPVDVASVSVSGSAAAEFTVTFNDCPAQLAEGATCN
                     VRVAFAPTAADTRSASLDVMGPEVASSTLLGHAFVRNARLIFTPGARDFGDLPQGTAS
                     DPFTFTVTNELATATFAASLAGPDANVFTIDSTDCNGAIPLHGTCSVTVKMTPPYGST
                     FVASLKLVDTGTGVWQAGLVGKSSLPIGLSPATGLFGSYLVGQAEQGGRVTYTLTNNG
                     DTTTGTLTPSLIGSDFTIKSTTCTTLSPLASCNVIVELTPTTRGAKTAQLQVTDGTSS
                     ARATTRGDAYTVLATGPASFPNTASGQTSAQQAYTIVNASDHATGAVSASITGQFAIA
                     SNGCSGALAA"
BASE COUNT          446 a          876 c          802 g          410 t
ORIGIN      
        1 gtgaagccgc gcagctcgca aaagccgacg aaggtcgctg cgaaagcgcc ggtgacagct
       61 gcgccgatcg tagctgcggc cacgccgagc gaagctgcgc cgatcgaagc tgcgccggtg
      121 ccgctcgtcg cgccagtcgc cgtcggagcg cccgaatccg caccgccgct accgctcgaa
      181 gtcgtgatcg gcgatgacac cgcgccgctg ctcgcgctcg atcagaagcc gatgaccgaa
      241 gccgagctcg tcgtcgagta tcagcgcgtc ggtcgcgaca tcgccaagct gcagaaggat
      301 cgcggcaccg acaaggtcct cgatctgtgg ccgacgtttc gctcgatcaa gctcaaagag
      361 tcctgcaaga cggccgagac gcgcctcaac ctcgagggca cgctcaagca acttcgctcg
      421 cacatcgatc gccggcgcgg gatcacgatc tctgcggact gcatgaacaa cccgctcgcg
      481 gcgaactgcc agtagtcgga gctgattaga accgacggcc cattacgagg ccgccgccgt
      541 tcttgttcgc cgtcggtgcg atgacgagcg cgcgcgcatc ctgctgcttg cgatcgtggc
      601 ggccgacgac gtagaggatg ccgccggtga tcatggtcgc gccgcccgcg aggagccaga
      661 gcttctggtt gcgctggtgt gcgtccgctt gcgcggcgag gtcttcgacg ttgacggtcg
      721 gatcgttgac gatctgcgcg ccgcgaactc gcgcgacctc gcgaccttcg tagagcgcca
      781 tgccgaggcc gccgataccc gcggcgagca tcgagaggcc gacgacctcg cgcttcgtga
      841 tgcgcgcagc cggcggagga atccgcaggt cgagcgcttc gacgcccgtg accggcttcg
      901 cgacctcgcc cttcttcgga ttgcacgcct cgtgatccgt agcgccggaa tgcccgagcc
      961 tgacggccag ctcgcgcgct ccgagcgatg cgcgatcggc atcttcgccg gcggcgtcga
     1021 cgtacatata cgagcagtag tagtcgagcg cttcgcgcat ctgaccggcc gcccgatatt
     1081 cgtcggcgat cagcgtgagc agatgcggat cgctcctctg ttcgtactgc tcgcgataat
     1141 gttcgatcgc gcggtcgtgc gttccagcga acgcgttcgt ggatgccgca gcacatagcg
     1201 ccgcaacgat gaaggtcccc aacttcatgc agcccatact acgcaacgcg ctcgcggact
     1261 gcgatccaaa tcttcgtcgg gaaatttcgc agcgcgcgct gtccggaaca tgagaaactg
     1321 tcaatcgtgg gccgtttcac ccagttcttc ttggtcgtgg gcctcgtagc ctgcggcgat
     1381 gacgcgcaaa caccgcagga tgcagccacg ccggatgcca gcgcggcgct cgtgctcgag
     1441 ccagcgtcct tcgacttcgg cgacgtcgag gtcggcgtgc cgtcgacggc gacgcacaac
     1501 tttgtcgtgc gcaacacatc tacagcgcct gtcgacgtcg cgagcgtgag tgtgagcggc
     1561 tcggcggcgg ccgagttcac ggtcacgttc aacgattgtc cagcgcagct cgcagaaggg
     1621 gccacgtgca acgttcgcgt cgcgttcgcg ccgaccgccg ccgacacgcg ctcggcatcg
     1681 ctcgatgtga tgggcccaga ggtcgcgagc tcgacgctgc tcggtcacgc attcgtgcgc
     1741 aacgctcgct tgatcttcac gccgggcgca cgcgacttcg gcgacctgcc gcaaggtacc
     1801 gcgagcgatc cgttcacgtt caccgtcacg aacgagctcg cgaccgcgac gttcgcagca
     1861 tcactcgccg gccccgacgc gaacgtattc acgatcgatt cgacggactg caacggagcg
     1921 atcccgctgc acggcacgtg ctccgtcacc gtcaagatga cgccgcccta cggctcgacg
     1981 tttgtcgcga gcctgaagct cgtcgatacg ggcaccggcg tgtggcaagc cggcctcgta
     2041 ggcaagagct cgctgccgat cggcctctcg ccggcgaccg gcctgttcgg ctcgtatctc
     2101 gtcggccaag ccgagcaagg cggccgcgtc acatacacgc tcacgaacaa cggagacacg
     2161 acgaccggca cactcacgcc atctctcatc ggctccgact tcacgatcaa gtcgacaacc
     2221 tgcacgacgc tatctccgct cgcgagctgc aacgtcatcg tcgagctcac gcccacgacg
     2281 cgcggcgcga agaccgccca actgcaagtc accgatggca cgtcgagcgc acgggcgacg
     2341 acgcgcggtg acgcgtacac cgttctggcg acgggaccgg cgtcgttccc gaacacggcc
     2401 agtggccaga cgagcgcgca gcaggcgtac acgatcgtca atgcgagcga ccacgcgaca
     2461 ggtgcggtgt ccgcctcgat caccgggcaa ttcgcgatcg cgtcgaatgg atgcagcggc
     2521 gcgctcgcag cgca
//
LOCUS       DATLBZ010000610         2974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8702463, whole genome shotgun sequence.
ACCESSION   DATLBZ010000610 DATLBZ010000000
VERSION     DATLBZ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8702463"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VMZ53_29935"
     CDS             complement(<1..317)
                     /locus_tag="VMZ53_29935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32773.1"
                     /translation="MRHLKTFLIAAIPFGVALGVVNGVRQGWKSGVGSGVLCGLLFGA
                     LITWFISRQARRFETLRPKYEPEGIVLDAPANLGGAGGWLILTKQRLVFEPHKLNVGA
                     KLTT"
     gene            complement(369..1022)
                     /locus_tag="VMZ53_29940"
     CDS             complement(369..1022)
                     /locus_tag="VMZ53_29940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HUS32774.1"
                     /translation="MNILLVRHGETAWNREGRYQGRTDIPLSETGQAQVRALGERLRH
                     VPIKIAYASPLSRAKNTAEAILGGRTPVVPLWLDANLLEISHGEWEGQLASDVEISHA
                     EMFGTWRSRPGRNSPAGPGAETLGDVETRAWAVLDKLCNELAENDTALIAAHDAVNRV
                     LICRILGLSLDRVWSFRQAPASLNVLSGPSIAELQVVRLNDSEHVAPLLQEAKHRAV"
     gene            1054..2061
                     /locus_tag="VMZ53_29945"
     CDS             1054..2061
                     /locus_tag="VMZ53_29945"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HUS32775.1"
                     /translation="MTHRSRILGVGMAVPERVVTNHDLAKVMETSHEWIVERSGIEQR
                     RWVSPGEDGAGLATKASKEAIERSGIEATDIDMIIYATLSPDVNFPGTGVFVQRALGL
                     GSKGIPCIDIRQQCTGFIYGISIADAYIRMGAAKNILVIGSEVHSTGLDVSTAGRDVT
                     VLFGDGAGAVVVSRATDEQHMILSTHIHADGTEAEILWTEYPASRAHPRITAEAMAER
                     KHYPVMKGKNVFKHAVTRMPQAIMEGMQANGIKSIAEVDMIIPHQANLRINQMVAQMI
                     GVPAEKTHNNIQKYGNTTAASIPICMHEAIELGKIKPGNLVCLVAFGAGLTWGSVFLR
                     Y"
     gene            complement(2055..>2974)
                     /locus_tag="VMZ53_29950"
     CDS             complement(2055..>2974)
                     /locus_tag="VMZ53_29950"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32776.1"
                     /translation="SVRRALDVLAQVADALDHIHKRGVIHGDIKAENLILAEEPVAGG
                     SRRRRIVRLLDFGLAKRFGTQDTELNGTPHYLAPERCTGGVASVASDIYALGVLGYLL
                     LTRTMPFDGNLAQILDAHVREAPPSLAERRGEDLDDAIEALVMRALAKQPALRHPSAA
                     AFRYELNNVMHMLEMTRRRQSNTSLKLDRKSDAAAVLFEQSMLAQVVVRGDGTIRLAN
                     SAFGELVEDPGASFKNRSLFDTPLALWVPDLKRALDRVKRKHKPMELRARRWSNELAL
                     WLTPGPINEDIHILVQNHPIVREPVRKTQ"
BASE COUNT          537 a          919 c          988 g          530 t
ORIGIN      
        1 gtggtgagct ttgcgccgac gttgagcttg tgtggctcga acacgaggcg ctgcttcgtc
       61 aagatcagcc agccgcctgc gccgccgagg tttgcgggcg catcgagaac aatgccttcc
      121 ggctcgtact tggggcgtag cgtttcgaag cggcgcgcct gcctcgagat gaaccacgtg
      181 atcaacgcgc cgaacagtag accgcagagg acgcccgagc cgacgccgct cttccagcct
      241 tgccgcacgc cattcaccac gccgagtgca acgccgaacg ggatggcagc aatcaggaat
      301 gtcttgaggt ggcgcatggc ggcctgtgga tgatgctgtg aactgtgtgg atgaatccgg
      361 atcggctttc agacggcgcg gtgcttcgcc tcttgtagca ggggcgcgac gtgctcgctg
      421 tcgttgaggc gaacgacctg cagctccgcg atggacgggc ccgagagcac gttgagcgac
      481 gcgggcgctt ggcgaaatga ccacacacgg tcgagcgaga ggccgaggat gcggcagatg
      541 agcacgcgat tcactgcatc gtgagcggcg atcagcgcgg tgtcgttttc cgcgagctcg
      601 ttgcacagct tgtcgagcac agcccacgcg cgcgtctcga catcaccgag cgtctcggcg
      661 ccggggcctg ccggagagtt gcgaccagga cgcgagcgcc acgtgccgaa catctcggcg
      721 tgcgaaatct cgacgtcact cgcgagctgg ccttcccatt cgccgtgtga aatctcgagc
      781 aggtttgcgt cgagccagag cggcaccacg ggtgtgcgtc caccgaggat cgcctcagcg
      841 gtgttcttcg cgcgcgagag cggcgatgca tacgcgatct tgatcggcac gtgacgaagc
      901 cgttctccga gcgcgcggac ttgtgcttgc cccgtttcgg aaagcggaat gtctgtgcgg
      961 ccctgatagc gaccttcgcg gttccacgcg gtttcgccat gtcggaccag aaggatgttc
     1021 atgcgcggac tgtatcgtgg tatccgagcg agcatgactc atcgcagccg catcctcggc
     1081 gtcggcatgg ccgtgcctga acgcgtcgtc acgaatcacg atctcgcgaa ggtgatggag
     1141 acgtcgcacg agtggatcgt ggagcgtagc ggtatcgagc agcgtcggtg ggtgtcgccc
     1201 ggtgaagacg gcgcaggcct cgcgacgaag gcgtcgaagg aagcgatcga gcggtcgggc
     1261 attgaggcga ccgacatcga catgatcatc tacgcgacgc tgtcgccgga cgtgaacttt
     1321 cccggcaccg gcgtgttcgt gcagcgcgca ctcggtctcg gcagcaaagg gatcccgtgc
     1381 atcgacattc gtcagcagtg caccggcttc atctacggca tctcgatcgc cgacgcgtac
     1441 atccgcatgg gcgccgcgaa gaacattctc gtgatcggct cggaagttca ctcgacgggt
     1501 ctcgacgtct cgaccgcagg ccgcgatgtg acggtgctct tcggtgatgg cgccggcgcc
     1561 gtcgtggtga gccgcgcgac cgacgagcag cacatgatcc tctcgacgca catccacgcc
     1621 gacggcaccg aagccgagat cttgtggacg gagtatccgg cgagtcgcgc ccatccgcgt
     1681 atcaccgcgg aagcgatggc ggagcgcaag cactaccccg tgatgaaggg caagaacgtc
     1741 ttcaagcacg ccgtcacgcg catgccgcag gcgatcatgg agggcatgca ggccaacggc
     1801 atcaagagca tcgccgaggt cgacatgatc atcccgcacc aagcgaacct gcggatcaat
     1861 cagatggtcg cgcagatgat cggcgtgccc gcggagaaga cgcacaacaa catccagaag
     1921 tacggcaaca cgacggcggc ctcgattccg atctgcatgc acgaagcgat cgagctcggc
     1981 aagatcaagc ccggcaacct cgtgtgcctc gttgcattcg gcgcgggcct cacgtggggc
     2041 tcggtcttcc tgcgctactg agtcttgcgc acgggctcgc gcacgatcgg atgattctgc
     2101 acgaggatgt gaatgtcctc gttgatcggc ccgggcgtca gccacagcgc tagctcgttg
     2161 gaccagcgcc gtgctcggag ctccatcggc ttgtgcttgc gcttcacccg gtcgagtgcg
     2221 cgcttgagat cgggcaccca cagcgcgagc ggtgtgtcga acagcgaacg attcttgaag
     2281 gacgcgcccg gatcttcgac gagctcaccg aacgccgagt tcgcgagccg gatggtgcca
     2341 tcgccgcgca cgacgacctg cgcgagcatc gattgctcga acagcacggc cgccgcatcg
     2401 gacttgcggt cgagcttgag cgaggtgttc gactgacgac ggcgcgtcat ctcgagcatg
     2461 tgcatcacgt tgttgagctc gtagcggaac gctgccgcgc tcggatgtcg aagtgctggc
     2521 tgcttcgcta acgcacgcat gacgagtgct tcgatcgcgt cgtcgagatc ttcgccgcgg
     2581 cgctctgcca agctcggtgg tgcttcccgc acgtgcgcgt cgaggatctg tgcaaggttt
     2641 ccgtcgaacg gcatcgtgcg tgtgagcaag agatatccca gcacgccgag cgcgtagatg
     2701 tcgctggcga cgctcgcaac gccgccggtg cagcgctcgg gcgcgagata atggggcgtg
     2761 ccgttgagct cggtatcctg cgtcccgaat cgtttcgcga gaccgaagtc gagcaggcga
     2821 acgatgcgtc gtcgccgcga gccacctgcc actggctcct cggcgaggat gaggttctcc
     2881 gccttgatgt cgccatgaat gacgccacgc ttgtggatgt gatcgagtgc gtcggccact
     2941 tgcgcgagga catcgagagc gcgccgcacg ctca
//
LOCUS       DATLBZ010000611         3626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10126677, whole genome shotgun sequence.
ACCESSION   DATLBZ010000611 DATLBZ010000000
VERSION     DATLBZ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10126677"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VMZ53_29955"
     CDS             <1..211
                     /locus_tag="VMZ53_29955"
                     /inference="COORDINATES: protein motif:HMM:NF027116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32777.1"
                     /translation="DHATGAVSASITGQFAIASNGCSGALAAHDSCTIQVTFKPTSTG
                     AKTGSLQVSASPGGSDTFPLAGTGI"
     gene            complement(208..1413)
                     /locus_tag="VMZ53_29960"
     CDS             complement(208..1413)
                     /locus_tag="VMZ53_29960"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS32778.1"
                     /translation="MSYDVIIVGARCAGAPTAMLLARAGYKVLVVDRSTFPSDTVSTH
                     VIHPQGVAALARWGLLDRLVATGCPAIETYVFDFGPFAVTGKPGTDESPVAYCPRRTI
                     LDKLLVDAAVEAGAEVREGFIVDEILRDDGVVIGIRGHNRDGVGVTEHARVVVGADGR
                     NSLVAKAVEPEQYNERPEFVCAYYAYWSNLPMHGRFETYIRPEHGFAAAPTHDGLTLV
                     VGGWPREQFEQRKRDLDASYESLFDMAPSFAQRIRVARRESKIFGAISPNFFRKPFGR
                     GWALVGDAGYTKDPITAQGISDAFRDAELLTAALASTFAGKQSFDAALGAYHSARDAQ
                     SMAMYDFTCQIAAMQPPPPELQQLLGAVQGNQDAMDGFCRVNAGVTSPAQFFSAENVG
                     KIFAAAAAR"
     gene            complement(1410..2180)
                     /locus_tag="VMZ53_29965"
     CDS             complement(1410..2180)
                     /locus_tag="VMZ53_29965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS32779.1"
                     /translation="MEQAQKLPDFADIFERLLVPAIFDRYARDLVERARPFGPSDRIL
                     DLGCGTGIVARVLRERLGGAARLTGLDVNAEMLAKARSIAPEIEWIEGNAAALPFADR
                     SFEVVLAQQVLQFVPDRLQALGEIRRVLVPGGRLIASTWRPREHQPLFGALGAVAEKH
                     LGAGNDKRWTLDGDALSTLLASAGFTDIQLDTASLEERHAMFPIQASTSAAGYTNVDT
                     ASMAAIEAESKAILARHTATDGTITNRSVTNVAIARVA"
     gene            complement(2220..>3626)
                     /locus_tag="VMZ53_29970"
     CDS             complement(2220..>3626)
                     /locus_tag="VMZ53_29970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32780.1"
                     /translation="RLIAAYVRLAMDHLELADMVGYAQVAIAYTRLAERFGAAAGPWR
                     VPLMHSMLALARDDFAESQRQQDAAAALDPENPRARRARAFHRICFLRAAERHAHLRA
                     SIGELRSLWAAMPYGAKLAEARVAGSLMRVGADDEVRAIVATLTSEAWDEEINFISLA
                     EAVWCTADREHATRLLPGVRKYQTRWMAYWLDVEILEFPTARLVAYLEGIAGNWDACD
                     DLFATALRDVEQLGKRSGIARMKFELGDLMLRCKRDVDRARVLISEARVLAAEVHLDE
                     LVAMIDRRHRDRRHTQPPRKLRGELAIVREGEYYAITRGAATLRFKATRGFQYLEQLV
                     ERAGVDIHVLDLMGSADADRGDAGEVIDAKALHTYRERADALRDVLEDAEQRGDAIGA
                     EQARSELEALATELSRGTSLGGKVRRSESAVDRARSAVQRRIKDALDRIAEQDAELGA
                     WLRRVVHTGNYCSFQGNV"
BASE COUNT          631 a         1179 c         1174 g          642 t
ORIGIN      
        1 cgaccacgcg acaggtgcgg tgtccgcctc gatcaccggg caattcgcga tcgcgtcgaa
       61 tggatgcagc ggcgcgctcg cagcgcatga ctcgtgcacg atccaagtga cgttcaagcc
      121 aacgagcacc ggcgcgaaga ccggctcgct acaagtcagc gcgtcacccg gcggctccga
      181 caccttcccg ttggccggta ctgggatcta acgagcagcc gctgcagcga agatcttacc
      241 gacgttctcg gcggagaaga actgcgctgg cgacgtgacg cccgcgttga cgcgacagaa
      301 gccgtccatg gcgtcctgat tcccctgcac cgcaccgagg agttgctgaa gctctggcgg
      361 cggcggttgc atcgcggcga tctggcacgt gaagtcgtac attgccatcg actgcgcatc
      421 gcgcgcggag tgatatgcgc cgagcgcggc atcgaacgat tgcttgccag cgaacgtcga
      481 cgcgagtgcc gcggtcaaga gctcggcgtc acggaacgca tcactgatgc cctgtgccgt
      541 gatcggatcc ttcgtgtaac cggcgtcgcc cacgagtgcc caaccgcgcc cgaatggctt
      601 gcgaaaaaag ttgggactga tggcgccgaa gatcttcgac tcgcgtcgcg ctacgcgaat
      661 gcgctgggca aacgatggcg ccatgtcgaa cagcgactcg tagctggcgt ccaggtcgcg
      721 tttgcgctgt tcgaattgtt cgcgtggcca accaccgacg acaagtgtga ggccgtcgtg
      781 tgtcggtgcc gccgcaaagc cgtgctccgg ccgaatgtat gtctcgaacc ggccgtgcat
      841 cggcaggttg ctccaatacg cgtaatatgc gcagacgaat tccggacgct cgttgtactg
      901 ctcgggctcg acggccttgg caacgagcga gttgcgaccg tcggcgccga cgaccacgcg
      961 tgcgtgctcg gtgaccccga cgccgtcacg gttgtggccg cggatcccga tgacgacgcc
     1021 atcgtcacgc aggatctcgt cgacgatgaa gccctcgcga acttcggccc ccgcttcgac
     1081 ggcggcgtcg acgagcagct tgtcgaggat cgtgcggcga ggacaatacg cgacgggcga
     1141 ctcgtccgtg cctggctttc cggtcactgc gaatggaccg aagtcgaaca cgtacgtctc
     1201 gatcgcggga cagccggtcg cgacgaggcg atcgagcaaa ccccaccgag cgagggcggc
     1261 gacgccttgg ggatgaatca cgtgcgtcga gacggtgtcg ctcgggaacg tcgaacgatc
     1321 cacgacgagg accttgtagc ctgctcgcgc gagtagcata gcggtcgggg ctccggcgca
     1381 gcgcgcgcct acgatgatga cgtcgtagct catgccaccc tcgcgatggc gacgttggtg
     1441 actgagcgat tggtgatcgt cccgtcggtg gcggtgtgac gagcgaggat cgctttggac
     1501 tccgcttcga tcgcggccat cgaggcagtg tcgacgttcg tgtagccggc agcgctggtg
     1561 ctcgcctgga tcgggaacat cgcgtgacgt tcttccaggc tagcggtatc gagctggata
     1621 tccgtgaagc cggcgctcgc aagcagcgta gacagcgcat cgccgtcgag cgtccaccgc
     1681 ttgtcgttgc ccgcgccgag gtgcttctct gcgaccgcgc cgagcgctcc gaacaatggt
     1741 tgatgctcgc gcggccgcca cgtgctcgcg atcaagcgcc cgccgggaac gagcacgcgc
     1801 cggatctcgc cgagtgcctg tagtcgatcg gggacgaact gcaggacttg ctgtgcgagc
     1861 acaacctcga acgagcgatc ggcgaacggc agcgctgctg cgttgccttc gatccactcg
     1921 atctccggtg cgatgcttcg cgctttcgcg agcatttccg cgttgacgtc gagacccgtt
     1981 agccgcgctg cgccgccgag gcgctcgcgc aacactcgcg cgacgatgcc cgtaccacag
     2041 cctagatcca agattcgatc ggatggaccg aatggccgag cgcgttcgac gaggtcgcgc
     2101 gcgtagcgat cgaagatcgc cgggacgagc agccgttcga agatgtctgc gaagtcgggg
     2161 agcttctgtg cttgttccat gctctcttgg cgtgccgaac agcggaacgg ccgttccact
     2221 catacgttcc cctgaaagct gcagtaattc ccagtgtgta cgacgcgacg aagccatgcg
     2281 ccgagctcag catcttgctc cgctattcgg tctagcgcgt ccttgatgcg gcgttggacc
     2341 gcgctgcggg cgcgatcaac ggccgactcc gagcgccgga ccttgccgcc gagtgatgtg
     2401 ccacgggaca gctcggtagc tagcgcttcg agctcgctgc gtgcttgttc tgcgccgatt
     2461 gcgtcgcccc gctgctcggc atcctcgagc acgtcgcgaa gtgcatcggc gcgctcgcga
     2521 taggtatgca gggccttcgc atcgatgacc tcgccggcat caccgcgatc cgcgtccgcc
     2581 gatcccatga ggtcgagaac gtggatgtcg acgcctgcac gctcgacgag ttgctccaga
     2641 tactgaaagc ctcgcgtcgc cttgaatcgg agcgtcgcgg caccgcgtgt gatcgcgtag
     2701 tactcgcctt cgcgtacgat cgcgagctca ccgcgcaatt tcctcggcgg ctgagtgtgc
     2761 cggcgatctc gatgccgccg atcgatcatc gcgacgagct cgtcgaggtg gacctccgct
     2821 gctagtacac gtgcttcgct gatcagtacg cgtgcgcggt cgacgtcgcg tttgcaccga
     2881 agcatgagat cgccgagctc gaacttcatg cgcgcgatgc cggagcgctt cccgagctgc
     2941 tcgacatcac gaagcgctgt cgcaaacaga tcgtcacatg cgtcccagtt gcccgcgatg
     3001 ccttcgaggt acgcgaccaa gcgcgcagtt ggaaactcga ggatctcgac gtcgagccag
     3061 tacgccatcc aacgcgtttg atacttgcgc acgcccggca agagccgcgt cgcatgctcg
     3121 cgatccgcgg tgcaccagac cgcttcggcg agcgatatga agttgatctc ttcgtcccac
     3181 gcttcggagg tcaacgtcgc cacgatggcc cgcacctcgt catctgcgcc gacgcgcatg
     3241 agtgaaccag cgacgcgcgc ttccgcgagc tttgcgccgt acggcatcgc agcccacaag
     3301 ctgcgcagct caccgatgct ggcacgcagg tgcgcgtgac gttcggccgc ccgcaaaaag
     3361 caaatgcgat gaaacgcacg tgcgcgacga gcccgggggt tctcggggtc gagcgcggcc
     3421 gctgcatctt gttgccgctg cgactccgca aagtcatcgc gtgcgagcgc gagcatcgag
     3481 tgcatgagcg gcacacgcca cggcccggcc gctgctccga atcgctcggc gagccgcgtg
     3541 tacgcaatcg cgacctgcgc gtagcccacc atatcggcga gctcgagatg atccatcgcg
     3601 agccggacgt aggcggcgat gaggcg
//
LOCUS       DATLBZ010000612        15927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10130291, whole genome shotgun sequence.
ACCESSION   DATLBZ010000612 DATLBZ010000000
VERSION     DATLBZ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15927
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10130291"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VMZ53_29975"
     CDS             <1..1003
                     /locus_tag="VMZ53_29975"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HUS32781.1"
                     /translation="RYRYVFVEGEGRVARSRIGRFRAPLAADAAEPLTFGAICCTEKS
                     RSPGPLARAAERTDLDGFLFCGDNAYCEGAETLDDYRALYAAHYGRPEHVALRANNGM
                     SITWDDHEVKNDWNPESIDDAQLAAAFQTFFEHAPITETTQRRIWRSSRWGKTLEIFV
                     LDCRSERKPSTIPSSAPRYISPEQLAWLKAGLSASTATFKLILNSVPITNMPLPWDAY
                     PTDRWEAYGAQRTELLRHIDDNHIGGVLWLSGDFHLAFISHVSPSGPGATQREVLCGP
                     GGQSANSLVSTLQQPQFSFATGTNNYTTLHFDPASRSVTVTYIDGNGVKFHEENFVP"
     gene            complement(1000..2043)
                     /locus_tag="VMZ53_29980"
     CDS             complement(1000..2043)
                     /locus_tag="VMZ53_29980"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUS32782.1"
                     /translation="MSVASLPSPSPRLRRTSRIADLKVAATLVRAALDKDRPLIANLI
                     VTRRCNLSCGYCYEYDKVSPPVPLDVLKERVDHIARLRAVFVTLTGGESLLNPDVDQV
                     VAHIRAKGMVPFLNTNAYLLTKEWIDKLNKAGLYGMQISIDNVNPNEVSKKSLKTIKK
                     KLELLAKHARFRVRINCVLGSSPPAEAIEVARTVIGLGFDMNTSLVRAEDGTLLPVSE
                     EMRAAYEQIRGMGKRAPIFLDDDYTRPLMENGESEWKCRAGARTFHVDEFGLVHLCAP
                     RVGSPGKPIAQYTVEDIRYHFNEKKPCAARCPVAYARHASRLDGWRSQGGEALQLMAP
                     SAPLVQLRLSKAA"
     gene            complement(2651..2818)
                     /locus_tag="VMZ53_29985"
     CDS             complement(2651..2818)
                     /locus_tag="VMZ53_29985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32783.1"
                     /translation="MPRKQRFKPSRKPQSTQAVVSSQQVDEGKQISADQSVGPQSQAN
                     DEPVAREIERE"
     gene            2924..3823
                     /locus_tag="VMZ53_29990"
     CDS             2924..3823
                     /locus_tag="VMZ53_29990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32784.1"
                     /translation="MATDNNGDGNGNGNGSASNGNGSASTGSRDLSGHFARTEDHRNS
                     QFGRSQDDHEEHRRYEEERRRYEEARRRYDEAHQRYFTHRSNNSNSTGSNRSWVGGDE
                     RVDRPIADRNAEARNVERDHDRYAAGQATDRQIDRVTDARDRSPRFDTYRYDENVRSS
                     EDRRRGFHPVQDRYYLDGKYRDDYHEPEHDRRDRFADTDDNPRNAWRSWSGRKFTDDR
                     DHRDRNVGYDRPPLARDDRPRVRASYDERHGHPSVRAEIDYDQQWASRTERTDDNDAS
                     HMRGRDEDQDRSFIPRDEYGRRR"
     gene            complement(4043..4498)
                     /locus_tag="VMZ53_29995"
     CDS             complement(4043..4498)
                     /locus_tag="VMZ53_29995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32785.1"
                     /translation="MAGKQILIVALALAVTPMVGCKKKSNTTEQVQSPAGSALDPNTT
                     GDPNAPNPNNAQAGSAMGTEATGVGAADNPMAGSGSTGGTGSSGGGGGGAMGSGGGGG
                     GGAMGSGAGGGGGGGGAMGSGHGSGAMGSGAMGSGAGAGSAGASAHTKH"
     gene            4583..8125
                     /locus_tag="VMZ53_30000"
     CDS             4583..8125
                     /locus_tag="VMZ53_30000"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HUS32786.1"
                     /translation="MSEIDSSHPSIVVFIGHDGSAAQTVLSGLTNEPGFAIVSTIDAG
                     VLAKASGAKGATSALPIVDVKDATSISPNHVYALPTEVQSLLRPGAIAVENVDGPGHI
                     DRLLRSVADHYGASATVVVLGGRDGDGALGIKRVREAGGLTICQRVEDGPFADLPRAA
                     VLTGLIDVVLPLEEIATRLLQSNEPAPPDPRADDTLRDILAMIRIRTGHDFSWYKRAT
                     LYRRLARRMHVCQTPTIQDYHRMLREQPNELTHLLRDFLISVTNFFRDPEAFQAVTQL
                     ALPRMFRAKGVGDQVRVWVAGCATGEEAYSLGMLLSEYASRLPEPPQLQVFATDIDEE
                     ALHEARAGIYSEQIATDVSRERLERFFTRENGGYRVSKELREMMLFSPHNVLRDPPFS
                     RLDLVTCRNLLIYFNREAQERVLNTFHFALRNEGMLFLGSSESAEGMQQFAAVDAKNR
                     IFERRIAATGVAMDALSTTARWTPRPAFPMPAAERPAIGSFGELHHRAVELYAPPSVL
                     VNEDLDVVHFSEHAGQFLTIAGGEPTRQILRMAHPALRFELRGALYAARQPNCNNDTR
                     VVRFEEDGRSRAIQIRVRAVDLAEAGHGTVLVIFDELPAQPQMPTAQSTDGENNEPMM
                     REMEEDLHRTRDQLRTTVEQYETSLEELKASNEELHAINEELRSATEELETSKEELQS
                     VNEELTTLNHELKVKVDEVSRANSDLQNLMTSTDIGVLFLDRQLNVKRFTPRICELFN
                     LIPSDIGRPLLHITHRLDYGDLPQAAAQVLLDLRTTDREVSSVDGRRFLVRFLPYRSI
                     EDRIDGVVLTFVDITDLKTAQEARTRSEAALRTTQERLSIALAAAPLAVFTHDSSLQV
                     TWAFAQGRQVDPNLAAHAFAAADAERYMSIARDVYASGVAQRAELDVNSPNGVRTFDF
                     RFEPRREGNLVQGVTAVGFDITESKLAENKLRETDRHKDEFIAMLSHELRNPLTPLRI
                     AFDIARRSTQDPKQMQNALSVMDHQLQILHRLVDDLLDLSRVAQGKMQLVHALVDSVK
                     IVEAALESTRPKLDEHHHKLELDLPDERLAVLGDFTRLTQVLTNLLMNSAKYTPDFGH
                     IKLSIRADRAQHLVSIEVRDTGVGISRELLPRVFDLFVQGEKNDNTRQGGLGIGLNLA
                     RRIVELHGGKISAFSDGPGAGSVFTVQLPLAKGT"
     gene            8125..8514
                     /locus_tag="VMZ53_30005"
     CDS             8125..8514
                     /locus_tag="VMZ53_30005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS32787.1"
                     /translation="MRVLVVDDYPGAADIACILVRMLGHDARGAKTGKQAVDEAAAFE
                     PDVIVLDIGLPDFDGYEVARRVRAQPGKRPFIAAMTGWDHPDRRALSLAAGIDMHVLK
                     PASEESLTNILEAAKKQLSEPDEALPA"
     gene            complement(8483..9493)
                     /locus_tag="VMZ53_30010"
     CDS             complement(8483..9493)
                     /locus_tag="VMZ53_30010"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HUS32788.1"
                     /translation="MAVPALAAERRDLIVVGTSAGGIEALPRLLGQLPATFNASMVIC
                     MHLGAREDAQLVHILERSTVLPVSWAEQGEPFLPGHIYVAPPDTHLSIYEGHLRLSSG
                     PRENFSRPSIDRLFRSAAAQQGARTIGVLLTGMMGDGVAGLVAIRDSGGRTIVQDPVD
                     AEFSELPTRALEALKPDAKLRVDEMGAALLDLTQEVAPHFAPPREIVLEAEIDRLGTA
                     SPREMDLLGSRVQQMCPECGGPLWEIGKVEHKHWRCYLGHVVNARDLIDNSSEQLEAA
                     LWSAIRSLHERAATWDALARDAREAGIERVASDYAARASEGREQAELARKFMLEVLRR
                     AR"
     gene            9550..10311
                     /locus_tag="VMZ53_30015"
     CDS             9550..10311
                     /locus_tag="VMZ53_30015"
                     /inference="COORDINATES: protein motif:HMM:NF028434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HUS32789.1"
                     /translation="MATVLFPLPDRDFDVTEVAVPWKLLREAGHTPVFATEAGATPSC
                     DPLLLTGVVFGKLGARADAIACYRELEQAPEFTTTRAWSELRADQFDALFLAGGHAPG
                     MKQYLGSADVQRITAEFFATGKPIAAICHGVLVAARSKRADGHSVLHGVRTTCLPKYM
                     ERAAFFSTFWRRGRYYRTYPLYVEDEVRAALAAPDDFVRGPRELSKRGTREDDTHAFV
                     VEQDNYVSARWPGDAWLIGKKLVERLAAAAQQKTG"
     gene            complement(10318..12114)
                     /locus_tag="VMZ53_30020"
     CDS             complement(10318..12114)
                     /locus_tag="VMZ53_30020"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HUS32790.1"
                     /translation="MSVPIEHYALIGDTRTVALVDRRGSIDWLCLPRFDAGACFASLL
                     GDSRNGHWQIAPTTSEVTLSRRYRDHSLVLETDFATQGGKVRLVDCMPADEDIPNVVR
                     VVEGIEGEVRMRMELVIRFDYGWSVPWVRKLDGALVAVAGPDGLALRTPVETRGENLT
                     TVAEFTVRAGERVPFVLTWFPSTGPVPAEIDAFEAIDACEKWWCAWASQCTYKGEWSE
                     AVERSLLTLKALTYTPTGGIVAAPTTSLPEKIGGNRNWDYRYGWLRDATYTLYALLVS
                     GFRDEARAWRDWLLRAVAGDPSQLQVMYGVAGERRLLEQELSWLPGYEGSRPVRIGNA
                     AVHQLQLDTYGEVIDALFHARRVGLDGDGFAWSLEMKLLEFLETAWQLPDEGIWEVRG
                     PRQHFTHSKVMAWVAFDRAVKSVEQFQVEGPVDRWRKIRDTIHAEVCERAFDSNKRAF
                     TQSYGSPALDAALLVMPQVGFLPHDDPRIISTVAAIERELLRDGFVIRYLTERGVDGL
                     PPGEGVFLLCTFWLADNYAMTGRMREARALFKKLLAIRNDVGLLAEEYDPRAQRMVGN
                     FPQAFSHVGLINTALNLDAETPSPTRERETRK"
     gene            12178..12603
                     /locus_tag="VMZ53_30025"
     CDS             12178..12603
                     /locus_tag="VMZ53_30025"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUS32791.1"
                     /translation="MLDADIDLDFRALADFRYQLRKFLAFSEQTARAVGLEPRQHQLL
                     LAIRGLPEDTEPTIATIAERMALRHHTVVELVDRLESAGLARRTRDPEDRRRANVTIT
                     ARGNDLLRKLSLAHRDELRTSAPALVASLGRVLRSAKKA"
     gene            12607..14259
                     /locus_tag="VMZ53_30030"
     CDS             12607..14259
                     /locus_tag="VMZ53_30030"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HUS32792.1"
                     /translation="MKEYQDELRDFTATKRLIPITMIAIAIGIIASFVALALLRLIGL
                     FTNLFFYLRWDTDFVSPAGHHLGIGVIVVPVIGALLIGVMARFGSEKIRGHGIPEALE
                     SILMRGSRVEAKLAVLKPLSAAISIGSGGPFGAEGPIIMTGGAFGSLIAQRFHLTSAE
                     RKTLLVAGSAAGMAATFGTPLAATLLAVELLLFEWKPRSFVPVAMACGAAMATRHFLF
                     DTAPLFPVTAPIAPDNIMVLVACVGAGIAAGVLSAAMTASVYFFEDIFLKLRIHWMWW
                     PAIGGLAIGIGGIIEPRALGVGYNSIADLLHGNLTLAAIVTLVLVKWAIWSISLGSGT
                     SGGVLAPLLMLGAALGAILAHVLPAAPVGFWPLIAMGAILGGTMRSPLTGILFAAEVS
                     GDFSALLPLTVAVVCAHAFTVLVLRRSVLTEKVARRGYHVTREYEIDPLEVLFVREVM
                     KPATSAVQLEHIASPNETLRSATYRMANHRVTTLSVVDGGTQIGVIELEDVLVARRRH
                     AEEETRREQVTPLVPGWIPLPPLVPQAVDRLERLSFRRKRPR"
     gene            14272..15507
                     /locus_tag="VMZ53_30035"
     CDS             14272..15507
                     /locus_tag="VMZ53_30035"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HUS32793.1"
                     /translation="MSSALLITGVKDTAVDPTERARVLAARRRLKMARSAHAYVRGST
                     RHFYEWLGKASVKLPVGPEIWICGDCHVGNLGPVGKVAGTPDIELRDLDQTAVGNPAY
                     DLVRLALSLAMAGRGSDLGGVTTARMAEDLIAGYELALSGDAPAASAAELPKPIRLVM
                     KRAVRRTWKKLFRERVGQGKRALPLGKRFWPLARVEREAVEQLVSIPELASLVTRLEH
                     RADNATVELVDAAYWVKGCSSLGMWRAAVLVDVVEPDNDKHSLCLLDIKEAGKTFAPH
                     VEGTPEDPAARVVMGARKLAPSLGERMAAVHMLDRSLFVRELMPQDLKVELDELSAED
                     ARGVAHCLGTVVGRAHGRQLAANDRASWLAEIAAHRSKTLDAPSWLWRAVVELVAIHE
                     AAYLEHCRLYALEPDKLVA"
     gene            complement(15504..>15927)
                     /locus_tag="VMZ53_30040"
     CDS             complement(15504..>15927)
                     /locus_tag="VMZ53_30040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32794.1"
                     /translation="GLATPTRLVPLRDDLLALGATATDLAALRGCSVAFEANNSAHLL
                     GWFFVAERMGLLSTLILRKLKRRLDAVVYDTATSYMNTCSRHAASRWIQLGTLLDRRS
                     ASPGMLERMEGGAHEAFQAQREWFGSFRMLGQAQARAC"
BASE COUNT         2725 a         5419 c         5109 g         2674 t
ORIGIN      
        1 gcgctatcgc tacgtcttcg tcgagggcga gggccgcgtc gcgcgcagtc gcatcggccg
       61 gtttcgcgcg ccactcgcgg cggacgcagc ggagccactg acgttcggcg cgatctgctg
      121 caccgagaag agccgctcgc ccgggccact cgcgcgcgcg gcggagcgca ccgacctcga
      181 cggctttctg ttttgcggcg acaacgcgta ctgcgaaggc gcggagaccc tcgacgacta
      241 ccgcgcgctc tacgccgcgc attatggccg ccccgagcat gtggcgttgc gcgcgaacaa
      301 cggcatgtcg atcacgtggg acgaccacga ggtgaagaac gactggaacc ccgaatcgat
      361 cgacgatgcg cagctggccg ccgcctttca gacgttcttc gagcacgcgc cgatcacaga
      421 gacaacgcag cgccggatct ggcgttcgtc gcggtggggc aagacgctcg agatcttcgt
      481 gctcgactgc cgcagcgagc gcaagccgtc gacgattccg tcgagcgcgc cgcgctacat
      541 ctcgcccgag cagctggcct ggctgaaggc cggcctgtcg gcgagcaccg ccacgttcaa
      601 gctcatcctc aactccgttc cgatcacgaa catgccgcta ccgtgggacg cctatccgac
      661 cgatcgatgg gaggcgtacg gcgcgcagcg tacggagcta ctccgtcaca tcgatgacaa
      721 tcacatcggc ggcgtgctgt ggctgtccgg tgattttcat ctcgcgttca tcagccacgt
      781 gtcgccgagt ggaccgggcg cgacgcagcg cgaagtcctc tgcggtcccg gcgggcagtc
      841 cgcgaattca ctcgtgtcca cgctgcagca accgcagttc tcgttcgcga ccggcacgaa
      901 caactacacg acgctgcact tcgatccggc gtcgcgctcc gtgactgtca cgtacatcga
      961 tggcaacggc gtgaagttcc acgaagagaa cttcgtccct taggcagcct tcgagagtcg
     1021 tagctgcacg agcggtgcgg acggcgccat cagctgcagc gcctcgccac cttgcgagcg
     1081 ccagccatcg agcctgctcg cgtgacgcgc gtaggcgacc gggcagcgcg cagcgcacgg
     1141 cttcttctcg ttgaagtggt agcgaatgtc ttcgaccgtg tactgcgcga tcggtttgcc
     1201 aggcgaaccg acgcgtggag cgcagaggtg gacgaggccg aattcgtcga cgtgaaacgt
     1261 gcgagcgccg gcgcggcact tccactcgct ctcgccgttc tccatgagcg ggcgcgtgta
     1321 gtcgtcgtcg aggaagatcg gcgcgcgctt gcccatgccg cggatctgtt cgtaggcggc
     1381 gcgcatctct tcgctcacgg gaagcagcgt gccgtcttcg gcgcggacga gcgacgtgtt
     1441 catgtcaaag ccaaggccga tcacggtgcg cgcgacttcg atcgcttcgg cgggcggcga
     1501 ggagccgagc acgcagttga tgcggacgcg gaagcgcgcg tgcttcgcca ggagctcgag
     1561 ctttttcttt atcgtcttga gcgacttctt cgagacctcg ttcgggttca cgttgtcgat
     1621 gctgatctgc atgccgtaga ggcccgcctt gttcagcttg tcgatccatt ccttcgtgag
     1681 gaggtacgcg ttcgtgttga gaaacggaac catgcccttc gcgcgaatgt gcgcgacgac
     1741 ttgatcgacg tcgggattga gcagggactc gccaccggtg agcgtcacga agaccgcacg
     1801 caggcgcgcg atgtgatcga ctcgctcttt gaggacgtcg agcggaacgg gcggcgagac
     1861 cttgtcgtac tcgtagcagt agccacagct gaggttgcag cggcgcgtga cgatgaggtt
     1921 cgcgatcagc gggcgatctt tatcgagggc ggcgcgcacg agcgtggcgg cgaccttgag
     1981 gtcggcaatg cgcgacgtgc gacgtaggcg tgggcttgga ctaggaagcg aggcgacgga
     2041 catttcccgg ggcctaccgc aacgtgcgtg ccgctcgcat gtacgcgaat tcccgtcggt
     2101 ctaggtgatg gaatgcgtcg aactctcgac gctgaaccga actgagcgtt tgaaaggcag
     2161 cagccaccac gcgcgatggc aaagcgctgc gcgcccgcac gttctagacg acagacaacg
     2221 cgttttcgcg gacgcgcgcg ggcactccgg ctgcaacacc gagacgcaag gaggagaagc
     2281 gacgagacga tcgatcacgt gctctggttc gagctcgttc gcgaagcacg cgccgccgac
     2341 ggccgcgagc atcgccttcg agacggagat cctcgacgac gacaggcacg accccgatcg
     2401 atgttttgca acgagcagtg cgagcctcgc acgctgattc aggcgacgtc ctcgaacacg
     2461 cagccccggt ggtctcacga ggcatgcacg tggcgtcagt ctcgaatcgc ggcgaggcga
     2521 agccgtccgc gcaaatgcgg cgctgatagc gcttgccgaa gacggctgag gatggcgccg
     2581 gggggcgtga ggaggttttc cgattggatg cgcgtgcggg agcgcgcggc gtccccctcc
     2641 cgcccgcgat ttattcgcgc tcgatctcgc gcgcgacggg ctcgtcgttc gcctggctct
     2701 gcggaccgac gctctgatct gcgctgattt gtttgccctc gtcgacctgc tggctcgaca
     2761 cgacggcctg ggtgctctgg ggcttgcggc tgggcttgaa gcgttgtttg cgcggcatgg
     2821 gcgacagtct ctgcaacggg tgtgcagaga tttctgtcac gccgcgtcgg catggcacgc
     2881 gcatcgcact cgggtagacc cctttcatct cacggagggc ccgatggcga cggacaacaa
     2941 cggcgacggc aacggcaacg gcaacggcag cgccagcaac ggcaacggca gcgcgagcac
     3001 cggctcacgc gacctcagcg gccacttcgc ccgcaccgaa gaccaccgca acagccagtt
     3061 cggccgctcg caagacgatc acgaagagca tcgccgctac gaagaggaac gccggcgcta
     3121 cgaagaggcg cgccgccgct acgacgaggc ccaccaacgc tacttcactc atcgcagcaa
     3181 caacagcaac agcaccggta gcaaccgcag ctgggttggc ggtgacgagc gcgtcgaccg
     3241 tccgatcgcc gaccgcaacg ccgaggcccg caacgtcgag cgcgatcacg atcgatacgc
     3301 cgctggccaa gcaacggatc gccagatcga ccgcgtgacc gacgcgcgcg accgcagccc
     3361 tcggttcgac acgtaccgct acgatgagaa cgtccgcagc tcagaagatc gtcgccgcgg
     3421 cttccacccc gtacaagatc gctactacct cgacggcaag taccgcgacg actaccacga
     3481 gcccgagcac gaccgtcgcg accgcttcgc cgacaccgac gacaacccgc gcaacgcttg
     3541 gcgcagctgg tccgggcgca agttcaccga cgatcgcgac caccgcgacc gcaatgtcgg
     3601 ctacgaccgc ccgccgctcg cgcgcgacga tcgtccgcgt gtccgcgcgt cgtacgacga
     3661 acgccacggc cacccgagcg tgcgcgccga gatcgactac gaccaacagt gggcatcgcg
     3721 caccgaacgc accgacgaca acgacgcgtc gcacatgcgc ggccgcgacg aggatcaaga
     3781 tcggtcattc atcccgcgcg acgagtacgg ccgccgccgc tagctcgcgc gccgcgggtc
     3841 gcccgcgatc agtgcttcgt gtggcccgat gcaccggcgc tgccggcacc ggcgcccgag
     3901 cccatcgcgc ccgagcccat cgcgcccgag ccgtggccag agcccatcgc gccgccgcta
     3961 gctcgcgcgc cgcgcctctc gcgcgacgat cttcgcgagc ccgcgcgacc agtcatcgcg
     4021 cgccactgcg acgtcatcgc gatcagtgct tcgtgtgagc cgatgcaccg gcgctgccgg
     4081 caccggcgcc cgagcccatc gcgcccgagc ccatcgcgcc ggagccgtgg ccagagccca
     4141 tcgccccgcc gccgccgccg ccgccgcccg cgccagagcc catcgccccg ccgccgccgc
     4201 ccccgccgga gcccatcgcc ccgccgccgc caccgccgct ggagcccgta ccgccggtcg
     4261 agccggagcc ggccatcggg ttgtcggccg cgccgacgcc agtggcctcg gtgcccatcg
     4321 cgctcccggc ctgcgcgtta ttcgggttcg gcgcgtttgg gtcgcctgtc gtgttcggat
     4381 cgagcgcgct gcccgcaggc gactggactt gctcggttgt gttgctcttc ttcttacaac
     4441 cgaccatcgg tgtcacggcg agcgcgagcg cgacaatcag gatctgtttt ccggccatac
     4501 gtcctcctct gcagcgcgtc agcaacatcc gtgcgagcac cttgcccgct atcggcgcct
     4561 gtggtctggt cgagcaacgc cgatgagcga gatcgactcg agccatccgt cgatcgtggt
     4621 cttcattggc cacgatggat ctgcggcgca gaccgtgttg tctgggctga cgaacgagcc
     4681 tggctttgcg atcgtgagca cgatcgatgc gggcgtgctc gcgaaggcga gcggtgcgaa
     4741 gggtgcgacg agcgcgctgc cgatcgtcga cgtgaaagac gcgacgagca tctcgccgaa
     4801 tcacgtgtac gcgttgccga cggaggtcca gtcgttgctg cggccgggcg cgatcgcggt
     4861 cgagaacgtc gacggacctg gccacatcga tcggttgctg cggtccgtgg cagaccatta
     4921 tggcgcgagt gcgacggtcg tcgtgctcgg cgggcgagac ggcgatggcg cgctcggcat
     4981 caagcgcgtg cgagaagccg gcgggcttac gatctgccag cgcgtcgaag atggcccgtt
     5041 cgcggatctg ccacgcgcgg ccgtgttgac cgggctcatc gatgtcgtgc ttccgctcga
     5101 ggaaatcgcg acgcgcctgc tccagagcaa cgagcctgcg ccgcctgatc cgcgtgccga
     5161 cgacacgctg cgcgacatcc tcgcgatgat ccgcattcgc accggccacg acttcagctg
     5221 gtacaagcgc gcgacgctgt atcgacgcct cgctcgccgc atgcacgtgt gtcagacgcc
     5281 gacgatccag gactatcacc ggatgctgcg cgagcagccg aacgagctca cgcacctctt
     5341 gcgcgacttc ttgatcagcg tgacgaactt cttccgcgat ccggaagcgt ttcaggcggt
     5401 cacgcagctc gcgttgcctc gcatgtttcg cgcgaagggg gtcggcgatc aggtgcgcgt
     5461 ctgggttgcc ggctgcgcga cgggcgagga agcctactcg ctcggcatgt tgctaagtga
     5521 gtacgcgtcg aggttgccgg aaccgccgca gctgcaggtt ttcgcgaccg atatcgacga
     5581 agaagcgctg cacgaggcgc gcgccggcat ctactccgag cagatcgcaa cggatgtgtc
     5641 gcgcgagcgt ctcgagcggt ttttcacgcg cgagaatggc ggctatcgcg tcagcaaaga
     5701 gctgcgggag atgatgctgt tctcgccgca caacgtcctt cgcgatccgc cgttttcgcg
     5761 gctggatctc gtcacgtgtc gcaatctgct gatctatttc aatcgcgagg cgcaggagcg
     5821 cgtgctcaac acgttccact tcgcgctgcg caacgagggc atgctgttcc taggttcgtc
     5881 ggaatccgcc gagggcatgc agcaattcgc tgcggtcgac gcgaagaacc ggatcttcga
     5941 gcggcgcatc gccgcgaccg gtgttgcgat ggatgcgctc agcacgaccg cgcggtggac
     6001 gccgcgtcct gcgtttccga tgccggccgc cgagcgcccc gcgatcggat cgtttggcga
     6061 gctccatcat cgcgccgtcg agctgtacgc gccgccgagc gtgctcgtaa acgaggacct
     6121 cgacgtcgtg cacttcagcg agcacgccgg tcagttcctc accatcgccg gcggcgagcc
     6181 aacgcgccag attctgcgca tggcacatcc ggcgttgcgc ttcgagctgc gcggtgccct
     6241 ctacgccgca cgccagccca actgcaacaa cgacacgcgc gtcgtgcgat tcgaggagga
     6301 cggcaggagt cgcgccatcc agatccgcgt gcgcgccgtt gatttggcgg aggcaggcca
     6361 cggcacggtg ctcgtgatct tcgacgagct gcccgcgcag ccgcagatgc cgacggcgca
     6421 atcgacggac ggcgagaata acgagcccat gatgcgcgag atggaggagg acctccatcg
     6481 cacacgcgac cagctccgta ccaccgtcga gcagtacgag acatcgctcg aggagctcaa
     6541 ggcctcgaac gaggagctac acgcgatcaa cgaggagctg cggtccgcga ccgaagagct
     6601 cgagacgagc aaagaagagc tgcagtccgt caacgaggag ctgacgacac tcaaccacga
     6661 gctcaaggtg aaggtcgacg aggtcagccg cgccaacagt gacctccaga acctcatgac
     6721 gtcgacggac atcggcgtgc tgttcctcga ccgccagctc aacgtgaagc ggttcacgcc
     6781 gcggatctgc gagctgttca acctgattcc ctcggacatc ggccgcccgc tcctgcacat
     6841 cacgcatcgc ctcgattacg gcgatctgcc gcaagcagcg gcgcaagtgt tgctcgatct
     6901 aaggacgacc gatcgcgagg tctcgagcgt cgacggtcgc cggttcctcg tgcgcttcct
     6961 tccgtatcgc tcgatcgaag atcgcatcga cggcgtggtg ctcacgttcg tcgacatcac
     7021 cgatctcaag acggcgcagg aggcccgcac acggtcggag gctgcgctgc gcacaacgca
     7081 ggaacggctg tcgatcgcgc tcgctgccgc gccgctcgcc gtcttcaccc acgattcgtc
     7141 actgcaagtg acgtgggcgt tcgcgcaggg ccgtcaggtc gacccgaacc tcgcagcgca
     7201 tgcatttgct gccgcagacg ccgagcgcta catgtcgatc gcgagggacg tctacgcatc
     7261 cggcgttgca cagcgcgccg agctcgacgt caattctccg aacggtgtcc gcaccttcga
     7321 tttccgcttc gagccacgac gcgaaggcaa tctcgttcag ggcgtcacgg ccgttggctt
     7381 cgacatcacc gagagcaagc tcgccgagaa caagctccgc gagaccgacc gtcacaagga
     7441 cgagttcatc gccatgctct cgcacgagct tcgtaacccg ctcacgccgc ttcggatcgc
     7501 gttcgacatc gcgcgtcgct cgacccaaga cccaaagcaa atgcagaacg cgttgtccgt
     7561 catggaccac caactgcaga tcctgcatcg gctcgtcgac gaccttctcg acctgtcgcg
     7621 cgtcgcgcag ggaaagatgc agctcgtcca cgcgcttgtc gattccgtca agatcgtcga
     7681 ggccgcactc gagtcgaccc ggcccaagct cgacgagcat caccacaagc tcgagctcga
     7741 ccttccggac gagcggcttg ccgtccttgg cgacttcacg cggctcacgc aggtcctgac
     7801 gaacttgctg atgaactcgg cgaagtacac gcccgacttc ggtcacatca agctgtcgat
     7861 tcgcgctgat cgcgcacaac atctggtctc gatcgaagtg cgcgacacgg gcgtcggtat
     7921 ttcccgcgag ctcttgccgc gcgtgttcga tctgttcgtc caaggcgaga agaatgacaa
     7981 cacgcgccaa ggcgggcttg gaattggcct gaacctcgca cgcaggatcg tcgagctgca
     8041 cggcggaaag atctctgcat tcagcgacgg tccaggcgcc gggagcgtct tcacggttca
     8101 gctacctctc gcgaagggaa catgatgcgc gtcctcgttg tcgacgacta tccaggtgcg
     8161 gcagatatcg cgtgcatcct cgttcgcatg ctcgggcacg acgcacgcgg cgcgaagacc
     8221 ggcaagcagg cggtcgacga agctgccgca ttcgagcccg acgtgatcgt gctcgatatc
     8281 ggcctgcccg atttcgacgg ctacgaagtg gcgcgtcgcg ttcgcgcgca acccggcaag
     8341 cgtcctttca tcgccgcgat gacggggtgg gaccaccccg atcgacgcgc cctctcgcta
     8401 gccgccggca tcgacatgca cgtgttgaag cccgcgagcg aagagagcct cacgaacatc
     8461 ctcgaagctg cgaagaagca gctcagcgag cccgacgaag cacttccagc atgaacttgc
     8521 gggcgagctc ggcttgctcg cgaccttccg aagcgcgcgc cgcgtagtcg ctcgctacgc
     8581 gttcgatgcc tgcttcccgt gcatcgcgcg cgagagcgtc ccaggttgcc gctctctcgt
     8641 gcagcgaacg aatcgcgctc cagagtgccg cctcgagctg ctcggaggag ttgtcgatca
     8701 gatcgcgcgc attgacgaca tggccgaggt agcagcgcca atgcttgtgc tcgaccttgc
     8761 cgatttccca tagcggcccg ccgcattccg gacacatctg ctgcacgcgc gagccgagca
     8821 gatccatttc gcggggagac gcagtcccga gtcgatcgat ctcggcttcg agcacgatct
     8881 ctcgcggcgg tgcgaagtgg ggagcgacct cctgtgtcag gtcaaggagc gcagcgccca
     8941 tctcgtcgac gcgcagcttc gcgtcgggct tcagtgcctc gagggcgcgc gtcggcagct
     9001 cggagaactc ggcgtcgacg ggatcctgca cgatcgtgcg accgccggag tcgcgaatcg
     9061 cgacgagacc agcgacgccg tcgcccatca tgccggtgag cagaacgcca atcgtcctcg
     9121 ctccctgttg cgcggcggct gatcggaaca gccgatcgat cgacggccgc gagaagttct
     9181 cgcgcggtcc gctggagagc cgcaagtgcc cttcgtagat cgacagatgc gtgtcaggcg
     9241 gcgccacgta gatgtggccc ggtaggaacg gttcaccctg ctcggcccac gagacaggga
     9301 gcacggtcga gcgctcgagg atatgaacga gttgagcgtc ctcgcgtgcg ccgaggtgca
     9361 tgcagatcac catcgacgcg ttgaacgttg ccggcaactg accgagaaga cgcgggagcg
     9421 cctcgattcc gcccgctgac gttccgacca caatcagatc acgccgctcc gccgcaagtg
     9481 ccgggaccgc catacgagct acaggttgca atgcttgtac cccccaacgc acgcgatatc
     9541 ttcgcaggca tggcaacggt cttgtttccg ctaccggatc gcgacttcga cgtcaccgaa
     9601 gttgctgtgc cgtggaagct gttgcgcgaa gctggccaca ctcccgtgtt cgcgacggag
     9661 gcaggtgcga caccctcctg cgatccgctg ctgctcacgg gcgtcgtgtt cggcaagctt
     9721 ggggcgcgcg cggatgcaat cgcgtgttac cgcgagctcg agcaggcacc cgaattcacg
     9781 acgacgcgcg cgtggagcga gctgcgtgcg gaccagttcg acgcgctgtt tcttgcgggc
     9841 ggtcacgcac ccggtatgaa gcagtatctc ggcagcgcgg acgtccagcg gatcaccgcc
     9901 gaattctttg ccacgggaaa accgattgcc gcgatctgtc acggcgtgct cgttgcggct
     9961 cgtagtaagc gcgcggacgg gcactccgtg ttgcacggcg ttcgcaccac gtgcttgccg
    10021 aagtacatgg agcgcgccgc gttcttctcg acgttttggc ggcgtggccg gtattaccga
    10081 acgtacccgc tctatgtcga agacgaagtt cgcgctgcgc tcgccgctcc cgacgacttc
    10141 gtgcgcggcc cacgcgagct ctcgaagcgc ggcacgcgcg aggacgatac gcacgcattt
    10201 gtcgtcgagc aggacaacta cgtctcggcg cgttggcccg gcgatgcgtg gctcatcggc
    10261 aagaagctcg tcgagcgcct cgcggcagcg gcgcagcaga agacgggctg accgctatca
    10321 ctttcgcgtc tcgcgctcgc gcgttgggct cggcgtttcc gcgtcgaggt tcagcgccgt
    10381 attgatgaga ccgacgtggg agaacgcctg cgggaagttt ccgaccatgc gctgcgcgcg
    10441 cggatcgtat tcctcggcga gcagccccac gtcattgcgg atcgcgagca gcttcttgaa
    10501 cagcgcgcgt gcttcccgca tgcgacccgt catcgcgtag ttgtcggcga gccagaacgt
    10561 gcagaggagg aagacgcctt cgcccggcgg cagaccgtcg acgccgcgct ccgtgagata
    10621 gcgaatcacg aagccatcgc gcagtagctc tcgctcgatc gcggcgaccg tcgagatgat
    10681 gcgcggatcg tcatgcggca agaatccgac ttgcggcatc acgagcagtg ccgcgtcgag
    10741 tgccggcgag ccgtacgact gcgtgaacgc acgcttgttc gaatcgaacg cgcgctcgca
    10801 cacctccgcg tgaatcgtgt cgcgaatctt gcgccaccga tcgacgggac cctcgacctg
    10861 gaattgctcg acgctcttca ccgcgcgatc gaacgcgacc cacgccatga ccttcgagtg
    10921 cgtgaagtgc tggcggggac cacggacttc ccagatgcct tcgtcgggga gctgccacgc
    10981 cgtctcgagg aactcgagca gcttcatctc gagcgaccac gcgaagccat cgccgtcgag
    11041 accgacgcgc cgcgcgtgaa acagcgcatc gatcacttcg ccgtacgtgt cgagctgcag
    11101 ctgatgcacc gctgcattgc cgatacggac gggacgcgag ccctcgtaac cgggcaacca
    11161 cgacagctct tgctcgagga gccgccgctc gcccgcgacg ccgtacatca cttggagctg
    11221 tgatggatca ccggcaaccg cgcgaagcag ccaatcacgc cacgcacgcg cttcatcgcg
    11281 aaagccgctg acgagcagtg cgtagagcgt gtacgtcgcg tcgcgcagcc agccgtagcg
    11341 atagtcccag ttgcgattgc cgccgatctt ctcgggtagc gatgtcgtcg gcgccgcgac
    11401 gatgcctccc gtcggcgtat aggtcagcgc tttgagcgtg agcagcgagc gttcgacggc
    11461 ctcgctccac tcgcctttgt acgtgcactg cgatgcccat gcgcaccacc acttctcgca
    11521 cgcgtcgatg gcttcgaacg catcgatctc ggcgggcacg gggccggtcg atggaaacca
    11581 cgtgagcacg aacggtaccc gctcaccggc gcgcaccgtg aactccgcga ccgtcgtgag
    11641 attctcgccg cgcgtctcga ccggcgtgcg cagcgcgaga ccatctggcc ccgcaaccgc
    11701 gacgagcgcg ccatcgagct tgcggaccca cggaacggac cagccgtagt cgaagcggat
    11761 cacgagctcc atgcgcatcc gcacttcgcc ctcgatgcct tcgacgacgc gcacgacatt
    11821 cgggatgtcc tcgtccgcgg gcatgcagtc gacgagacgc accttgccgc cctgcgttgc
    11881 gaagtcggtc tcgagcacga gcgagtgatc gcggtaacgc cggctcagcg tgacctcgga
    11941 cgtcgtcggg gcaatttgcc agtgcccatt ccgcgaatcg cccagaaggg aggcgaagca
    12001 cgcgcccgca tcgaaccgag ggaggcacag ccagtcgatc gatccgcgcc gatcgacgag
    12061 agccaccgtc cgggtatctc cgatcagcgc gtagtgttcg atcggtacgg acatagtgaa
    12121 gtctggaggg aaatcatccg tctggcattt tatatcgtat cacgatatgt atctcgcatg
    12181 ctcgatgccg acatcgacct cgactttcga gcgctcgcgg attttcgcta tcagcttcgc
    12241 aagttcctcg cgttctccga gcagaccgcg cgcgccgtcg gcctcgagcc tcgccagcat
    12301 cagctgctcc tcgcgatccg cggcctgccc gaggacaccg agccaaccat cgcgacgatc
    12361 gccgagcgca tggcgctgcg tcatcacacg gtcgtcgagc tcgtcgatcg cctcgagtcc
    12421 gcgggcctcg ctcgccgcac acgagatcca gaagatcgcc gccgcgcgaa cgtgacaatc
    12481 acagctcgcg gcaatgatct gcttcgcaag ctatcgctcg cacaccgcga cgagctgcgc
    12541 acgagcgcgc ctgcactcgt cgcgtcgctt ggccgcgtgt tgcgatcagc aaagaaagct
    12601 taggtcatga aggagtacca agacgagctt cgcgacttca cggcgacgaa gcgcctcatt
    12661 cccatcacga tgatagcgat cgcgatcggc atcattgcct cgttcgtcgc gctcgccctg
    12721 ctccgcctca tcgggctgtt cacgaacctg ttcttctacc tgcgttggga cactgacttc
    12781 gtgtcgcccg ccggccatca cctgggcatc ggcgtcatcg tcgtgcccgt catcggcgcg
    12841 ctcctcatcg gcgtgatggc gcgcttcggc tccgagaaga ttcgcggcca cggtatcccc
    12901 gaggcgctcg agtcgatcct catgcgcggc agccgcgtgg aggcaaagct cgcggtgctc
    12961 aagccactgt ccgccgcaat ctcgatcggc tcgggtggcc cgttcggtgc cgaaggcccg
    13021 atcatcatga ccggcggcgc gttcggctcg ctgatcgcgc agcggtttca cctcacgtca
    13081 gcggaacgca agacgctgct cgtcgcaggc tcggccgccg gcatggccgc gacgttcggc
    13141 acaccgctcg ccgcgacgct gctcgctgtc gagctcctcc tcttcgagtg gaaaccgcgc
    13201 agcttcgtgc cggtcgcgat ggcctgcggc gcagcgatgg cgacgcgcca cttcttgttc
    13261 gacaccgcgc cgctgttccc ggtcaccgcg ccgatcgcgc cggacaacat catggtcctc
    13321 gtcgcctgcg tcggcgcagg catcgcagcg ggtgtgctct ctgccgcgat gacggcgagc
    13381 gtctacttct tcgaggacat cttcctgaag ctgcgcatcc actggatgtg gtggccggcg
    13441 atcggcggtc tcgcgattgg catcggcggg atcatcgagc cgcgcgcgct cggcgttggc
    13501 tacaactcga tcgcggatct gctgcacggc aacctcacgc tcgccgcgat cgtgacgctt
    13561 gtcctcgtca agtgggcgat ctggtcgatc tcactcggct cgggaacgtc cggcggtgtg
    13621 ctcgctccac tgctcatgct cggcgctgcg ctcggcgcga tcctcgctca cgtgctgccg
    13681 gcagcgccgg tcggcttctg gccgctcatc gcgatgggcg cgatcctcgg cggcacgatg
    13741 cgctcgccgc tgaccggcat cttgttcgcg gcggaagttt cgggagactt cagcgcgctg
    13801 ttgcctctca ccgtcgccgt cgtctgcgcg cacgcattca cggtgctcgt cctgcgtcgc
    13861 tcggtgctga ccgagaaggt cgcgcgccgc ggctatcacg tcacgcgcga gtacgagatc
    13921 gatccgctcg aggtcttgtt cgtccgcgag gtgatgaagc cagcgacgtc cgcagtgcag
    13981 ctcgagcaca tcgcatcgcc gaacgagacg ctgcggagcg cgacgtatcg gatggcaaat
    14041 catcgcgtga caacgctctc cgtcgtcgat ggtggcacgc agatcggagt catcgagctc
    14101 gaagatgtcc tcgtcgctcg ccggcgccac gccgaggaag agacgcgacg cgagcaggtc
    14161 acgccgctcg tcccgggctg gatcccgctg ccgccgctcg tccctcaggc agtcgaccga
    14221 ctcgagcgcc tgtcgttccg gcgcaagcgg cctcggtaga tcgccgacgg catgtcgtct
    14281 gcactcctga tcacaggagt gaaagacacc gccgtagacc cgaccgagcg agcccgcgtg
    14341 ctcgccgcgc gacgccgtct caaaatggct cgcagcgcgc atgcctacgt gcgaggcagc
    14401 acgcgtcatt tctacgagtg gctcggcaag gcgagcgtga agcttcccgt cggtcccgaa
    14461 atctggatct gcggcgattg ccacgtcggc aacctcgggc ccgtcggcaa ggtcgccggt
    14521 acgcccgaca tcgagctgcg cgacctcgat cagacggcgg tcggaaatcc tgcgtacgat
    14581 ctcgttcggc tcgcgctctc gctcgcgatg gccggccgcg gctcagacct cggcggtgtc
    14641 acgacggcgc ggatggccga ggacctcatc gccggctacg agctcgcgct gtccggtgac
    14701 gcacctgcgg cctccgcggc ggagctgcca aagccgatcc gcctcgtgat gaagcgcgcg
    14761 gtgcgccgca cgtggaagaa gctgtttcgc gagcgcgtcg gtcaaggcaa gcgcgcgctg
    14821 ccactcggca agcggttctg gcctctggcc cgcgtcgagc gcgaagcggt cgagcagctc
    14881 gtgtcgattc ccgagcttgc atcgctcgtc acgcggctcg agcaccgcgc cgacaacgcg
    14941 accgtcgagc tcgtcgacgc ggcgtactgg gtgaagggct gcagctcgct gggcatgtgg
    15001 cgtgccgcag ttctcgtcga cgtcgtcgag cccgacaatg acaagcactc gctctgcttg
    15061 ctcgacatca aggaagccgg caagacgttc gcgccgcacg tcgagggcac gccggaggat
    15121 cccgcggctc gcgtcgtgat gggcgcgcgc aagctcgcac caagcctcgg cgagcgcatg
    15181 gccgccgtgc acatgctcga ccgatcgctg ttcgtgcgcg agctcatgcc tcaagatctc
    15241 aaggtcgagc tcgacgagct ctccgccgaa gacgcgcgcg gcgtcgcgca ctgcctcggc
    15301 acggtcgtcg gtcgcgccca cggccggcag ctcgcggcga acgatcgcgc gtcgtggctc
    15361 gccgaaatcg ctgcccatcg cagcaagacg ctcgacgcac caagctggct atggcgtgcg
    15421 gtcgtcgagc tcgtcgcgat ccacgaggcg gcgtacctcg agcactgccg gctctacgcc
    15481 ctggagcccg acaagctcgt cgcttaacag gcgcgggcct gcgcctgccc gagcattcgg
    15541 aacgagccga accactcacg ctgcgcctga aacgcttcgt gtgcgccgcc ctccatgcgc
    15601 tcgagcatcc ccggcgacgc cgagcgtcga tcgaggagcg tgccgagctg gatccagcgg
    15661 gatgccgcgt ggcgcgagca ggtgttcatg tagctcgtcg cggtatcgta gacgactgcg
    15721 tcgaggcgac gcttcagctt gcgcaggatg agcgtgctga gtaggcccat cctctcggcg
    15781 acgaagaacc agccgagcag atgcgccgag ttgttcgctt cgaatgcgac cgaacacccg
    15841 cgaagcgccg cgagatctgt cgccgttgca ccgagcgcga gcaaatcgtc gcgcagcggc
    15901 acgagccggg tcggtgtggc gagaccc
//
LOCUS       DATLBZ010000613         3725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8712594, whole genome shotgun sequence.
ACCESSION   DATLBZ010000613 DATLBZ010000000
VERSION     DATLBZ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8712594"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VMZ53_30045"
     CDS             complement(<1..872)
                     /locus_tag="VMZ53_30045"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HUS32795.1"
                     /translation="MLRTCVLAAVVCLVPALAIADDTSASDKLRILYSTRFTFTDDGV
                     PLITVEIMGNRKKVELRAKSGIVVRPDGSGGSAIETDSGDAWTISVENAKPAQIMEWT
                     IVETLGPDDEPGVNAALARWKQRGFDPRSFEIGTVFGTGGEVIDTREVRIAIDPVAQG
                     KGGARAADIAKKFGVKTSVHEELVRRPSGTIVAKSGSTVIRNPSVLWFAPKKATETIT
                     VPDVPTNTGGSQMETGSEERRYWGSVYVTLDHDGSLLAANAVSEDKLLAGLVPAEMYP
                     DAPQAALEAQAIAAR"
     gene            complement(930..2222)
                     /locus_tag="VMZ53_30050"
     CDS             complement(930..2222)
                     /locus_tag="VMZ53_30050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32796.1"
                     /translation="MRLYRVVVLVALAACRGHFDDRVDTSGIDATSRTAITLSVQGHG
                     VVLVDGHAPCADTCTYDDGVLNLSTWSGDGWQLSGFSNTCATATSCAAQPGESVTVSF
                     ARSPITANLAFVTTQLSPRTGLAGLDMTCATAASAAGLSGTFLAFASTSTVNARDRFA
                     SSRGWVRTDGLALYDTISDITLNTRPRSVSLDEIGMLIPDGTVAVTGTNENGNVNTQT
                     CNDWTSASAALQVTVIYPAWVGIYSVSNVNTSCNSPTRVFCMQNDRNVPLPPVAPAPM
                     GRYVFVSSQTWVPGGGIASADAECATEAAAANLSGTYAALLATTTMTAAEHVGGVRGL
                     FRRTDGVVVSFEGLDAPELDAAITMTAGGTFASAGTLDSILGASSLNVVGDGTCSDWT
                     DSTAVGRKGFFAVSTTPFARIFAPQCNFANRLVCVQLP"
     gene            complement(2248..>3725)
                     /locus_tag="VMZ53_30055"
     CDS             complement(2248..>3725)
                     /locus_tag="VMZ53_30055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32797.1"
                     /translation="ELVAKAPKSRFTGDDEYQFRHAIVREAAYAMLTDADRKLGHRLA
                     GDWLQYTGETEALTLAEHFERGGEPARAALWYRPAVEQALAAGDFRGAANRAERGVTC
                     GAKGETLGLLRRAQAEAHKWCGEFVEAERCALEAMRLLLAGSSPWFSAANEAAEASGK
                     QGDAEELQEIADALMGTPDLDGDLAPRVSAMANAAFQLYNNGRYQAATALVDKIDSFA
                     SEVRDPRILARIYQTRSSRAMFSGDSGAYVTSEVAAAHAFEQAGDDRYATMQHGHVGY
                     AYLEIGAYHDAAVWLRRALEGGLRMGLQNVVATAKHNLGRALMYQDQLDEALAIETEA
                     VEAFKAQGDRRLESASRSYLAEILAARGDLAAAEHELRLGLQTVQAASRPQQLAMLGH
                     VLLSMKRPREALIAAREAQALLDGLGGVEEGESLVRLIYAESLHASGELDAARSAVRV
                     ARDRVLERAAKITDPEWRATFLANLPENARTLALAKQLLSK"
BASE COUNT          611 a         1191 c         1264 g          659 t
ORIGIN      
        1 cgagcggcga tcgcctgcgc ttcgagagcg gcctgcggtg catcgggata catctcggca
       61 ggcacgaggc cggcgagcag cttgtcctcg gagacggcgt tcgccgcgag gagcgagcca
      121 tcgtgatcga gcgtgacgta cacgctgccc cagtagcgcc gctcttcgct gcccgtctcc
      181 atctgagatc cgccggtatt cgtcggcacg tcgggcacgg tgatcgtctc ggtcgccttc
      241 ttcggagcga accacagcac cgacggattg cgaatcaccg tcgagccgct cttcgcgacg
      301 atcgttcccg atggccggcg cacgagctct tcgtgcacgc tcgtcttcac gccgaacttc
      361 ttcgcgatgt ctgctgcacg cgcgccgcct tttccctgcg cgacgggatc gatcgcgatg
      421 cgaacttcgc gcgtatcgat cacttcaccg ccggtgccga acacggtgcc gatctcgaac
      481 gagcgcggat caaagccacg ctgcttccat cgcgcgagtg ccgcgttcac gccgggctcg
      541 tcgtcagggc cgagcgtctc gacgatcgtc cattccatga tctgcgccgg cttcgcgttc
      601 tcgacgctga tcgtccacgc gtcgccgctg tcggtctcga tcgcactgcc gccggagccg
      661 tccggccgca ccacgatgcc gctcttcgca cgcaactcga ccttcttgcg gttgcccatg
      721 atctccacgg tgatgagcgg cacgccgtca tccgtgaatg tgaagcgcgt cgagtacagg
      781 atgcgcagct tgtcggacgc ggacgtgtca tcggcgatcg cgagcgccgg aacgaggcaa
      841 accacagccg ccaagacgca agtgcggagc atggacgagg ctaggcaccg gccgatccgc
      901 agagcaagaa aacggccgaa tacgccccct taggggagtt gcacgcagac gaggcggttc
      961 gcgaagttgc actgcggtgc gaagatgcgt gcgaacggcg tcgtcgagac cgcgaagaag
     1021 cctttgcggc cgactgcagt gctgtccgtc caatcggagc atgtgccatc gccgacgacg
     1081 ttcaaggagc tcgcaccgag gatggagtcg agtgtgccgg ctgacgcgaa cgtgccgccg
     1141 gcggtcattg tgatcgctgc atccagctcc ggtgcgtcga ggccctcgaa cgacacgacg
     1201 acgccatcgg tgcgacggaa gaggccacgc acgccgccca catgctcggc tgcggtcatc
     1261 gtcgtggtcg ccagtagagc ggcgtaggtt ccggacagat ttgcagcggc tgcctcggtt
     1321 gcgcattccg cgtccgcgga tgcaatgccg ccgcccggca cccaggtctg cgacgagacg
     1381 aacacgtagc gacccatcgg cgcaggggcg acaggaggaa gcgggacgtt gcgatcgttc
     1441 tgcatgcaga agacgcgcgt cggactgttg caggatgtgt ttacgttgct gaccgagtag
     1501 atgccgaccc aagccgggta gataacggtc acctgcagcg cggcgcttgc tgacgtccaa
     1561 tcgttgcagg tctgggtgtt gacgttgccg ttctcgttcg tgccggtgac agcgacggtg
     1621 ccatctggga tcagcatgcc gatttcatcg aggctcacgg atcgcggccg ggtattcagc
     1681 gtgatgtccg agatcgtgtc gtacaacgcg aggccgtcgg tgcggaccca gccgcgcgag
     1741 ctggcgaagc gatcgcgagc gttcaccgtg ctcgtcgatg cgaaggcgag gaacgtgccc
     1801 gataggcccg cagccgaggc ggcggttgcg cacgtcatgt cgagaccggc aaggccggtg
     1861 cgcggcgaga gttgcgtcgt gacgaacgcg agatttgcgg tgatcggaga gcgcgcaaac
     1921 gagacggtca cggattcgcc gggctgagct gcacacgatg tcgccgtcgc gcaggtgttg
     1981 ctgaagcctg acagctgcca gccatcgccg gaccacgtcg agagattcag cacgccgtca
     2041 tcgtaggtgc acgtgtctgc gcacggagcg tggccatcga ccaacaccac gccgtggcct
     2101 tggacgctca gggtgatcgc ggtccgggag gtggcatcga tgccactcgt atccacgcga
     2161 tcgtcgaagt gcccacgaca ggccgcgagc gccacgagca cgacgacgcg gtacagcctc
     2221 acaccgcgat cgtagcgcta cttcgcgcta tttcgaaagc aactgtttgg cgagggcgag
     2281 cgttcgcgcg ttctcgggga ggtttgcgag aaaggtggcg cgccactcgg gatccgtgat
     2341 ctttgccgcg cgctcgagga cgcgatcacg tgctacgcga actgcggatc tcgcagcgtc
     2401 gagctcgccc gaggcatgca gagactcggc gtagatgagc cgcacgagtg actcgccctc
     2461 ttcgacgccg ccgaggccat cgaggagtgc ctgcgcttcg cgcgctgcga tcaacgcttc
     2521 gcgcggtcgc ttcatcgaga gcagcacatg gccgagcatc gccagttgtt gcgggcgcga
     2581 ggcggcttga acagtttgca agccgaggcg cagctcgtgc tccgccgccg cgaggtcgcc
     2641 gcgagccgcg aggatttcgg cgaggtacga gcgcgatgcc gattccaagc ggcggtcacc
     2701 ctgcgccttg aacgcttcga cggcttcggt ctcgatcgcg agcgcttcgt cgagctggtc
     2761 ttgatacatg agcgcgcggc caaggttgtg cttcgcggtg gcgacgacgt tttggaggcc
     2821 catgcgcagg ccgccttcga gagcgcggcg cagccagact gcggcgtcat ggtaggcgcc
     2881 gatttcgagg tacgcgtagc cgacgtgacc gtgctgcatc gtcgcgtagc ggtcatcgcc
     2941 tgcttgttcg aacgcatgcg cggctgcgac ctcggacgtg acgtacgcgc cggagtcgcc
     3001 agagaacatg gcgcgcgacg agcgtgtttg atagatgcgc gcgaggatcc gcggatcgcg
     3061 gacttcagac gcgaagctgt cgatcttgtc gacgagcgcg gtcgcggctt gatagcggcc
     3121 gttgttgtag agctggaatg cagcgttcgc catcgcggag acgcgcggcg cgagatcgcc
     3181 atcgagatcg ggcgtcccca tcagtgcatc ggcgatctct tgcagctcct cggcgtcgcc
     3241 ttgcttaccg ctcgcttccg cggcttcgtt tgccgccgag aaccacggcg acgagcccgc
     3301 aagtaacagg cgcatcgctt cgagtgcgca gcgttcggct tcgacgaact cgccgcacca
     3361 cttgtgcgct tccgcttgtg cgcggcggag caggccgagc gtttcgcctt ttgcgccgca
     3421 ggtgacgccg cgttcggcgc ggttcgcggc accgcggaag tcgcctgcgg caagcgcctg
     3481 ctcgacggcg ggccggtacc agagtgcggc gcgtgcaggt tcgccacctc gctcgaaatg
     3541 ctccgcgagt gtgagcgctt cggtctcgcc cgtgtactgc agccagtctc ctgcgagccg
     3601 atgaccgagc ttgcgatcgg cgtcggtcag catcgcgtac gcggcctcgc gcacgatcgc
     3661 gtggcggaac tgatattcgt cgtcgcctgt gaagcgagac ttcggtgcct tggccacgag
     3721 ctcgc
//
LOCUS       DATLBZ010000614        10249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8713160, whole genome shotgun sequence.
ACCESSION   DATLBZ010000614 DATLBZ010000000
VERSION     DATLBZ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8713160"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..775)
                     /locus_tag="VMZ53_30060"
     CDS             complement(173..775)
                     /locus_tag="VMZ53_30060"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HUS32798.1"
                     /translation="MIVTLGDDHPLVGDEHLIAASLGPARKRELAAGRTALRTALAEL
                     AAPSQLDASTLSAQPILADDRGAPILPAGFVGSISHKAERAAGLVAPAGRGFVGLDIE
                     LAAPPRMPIERRILTPREQAHVSGRDVTLYFSIKEAIYKAIDPIVRRYVGFTEIELDV
                     RSDGSVSVTVLDATRLPVEIEASWLERDGYWLATARGRRR"
     gene            complement(804..1997)
                     /locus_tag="VMZ53_30065"
     CDS             complement(804..1997)
                     /locus_tag="VMZ53_30065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32799.1"
                     /translation="MRGRLWVVGLLLLVTRLAHADGQGDEYAAQVKLMFRIAACGNTT
                     DAVPERLSEHSITGHCQRMNRIYAGYKKAWVDKAKPFIAKLRPTDVPATVVYPFGGGD
                     LSSALTVYPDATEITTISLEAAGDVRAVDSIDQKRLDADLDAISKDINRLYKAAHSTT
                     QSLQQASHSILPGTIMFALAGLAVHDMEPVQLRYFDLEKDGTLHYLTAKEMDDRAEKI
                     GRPYRTKKKWVEHFWYEQHSAFGNVEIQFRPRGDTKAAVRTYRHILANLDDKHMKDDD
                     RVLKHLASKGKVAVMTKAASFLLWWDDFSQIRDYLLANTQWMISDASGIPLRYAEKAG
                     YELVTYGDFAGPYFTQDPIGARKEFIKLWAEEPHRDLPFRFGYPDKDKKNHLMVMQPK
                     ARAKS"
     gene            2124..2831
                     /locus_tag="VMZ53_30070"
     CDS             2124..2831
                     /locus_tag="VMZ53_30070"
                     /inference="COORDINATES: protein motif:HMM:NF017519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HUS32800.1"
                     /translation="MKRLFVIAALVAAAATGCSEPHSVMVKRPQDPQVDAAVTAMWSH
                     EIHSVAQDGDWIVSRSYYLLADGLTIATTGEPLSHASIYDAKRDTIIESVGSGVREIP
                     LSELVERNHYIIVIHPSNMTAVQRRHSVERARSRMGTPFDIGGMFGFDDKDKVYCSEL
                     VWWASQGELRTGVHYMVVTPGDLLKHGEVVYWSGKRTDEQVMSIAMANESRDRAEQLA
                     TQEIAAEQLAPEQLAAK"
     gene            complement(2832..4184)
                     /gene="aroA"
                     /locus_tag="VMZ53_30075"
     CDS             complement(2832..4184)
                     /gene="aroA"
                     /locus_tag="VMZ53_30075"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HUS32801.1"
                     /translation="MKLIVEPATRQLSGTIVVPGDKSIGHRALLFSLLSQTPVRVLGL
                     GDGADNGRSAKAISALGASVSRDGKAIVIQGTGLDGMTAPSGAVDCGNSGTTIRMLCG
                     LLSGQRFATTLVGDESLSKRPMRRVIEPLMSMGATIKGQGGTAQGGGLDVTPPLVVGP
                     ATGALRALDYVLPMASAQVKTAVLLAGLYADGETRVTEPGPSRDHSERMLAYLGAPLR
                     VNGRTTTIETRGWDRRLAGAGFEVPSDPSSSAFLVAAALLVGAGEVRLPGICINPTRT
                     GFLDAIAAMGGEVALEDRDDSGPEPTATLVVRGGVRELRAMEIAGDLAVRSIDELPIL
                     AVIAARARGVTRVRDAEELRVKESDRIATTIAMLKSFGVNCEAHPDGFTVEGRPDGPL
                     EPGNVHADGDHRIAMAGAVAGLVASGTTHIGDADNVATSYPGFAAALTQLGATIRSEA
                     "
     gene            complement(4769..5734)
                     /locus_tag="VMZ53_30080"
     CDS             complement(4769..5734)
                     /locus_tag="VMZ53_30080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32802.1"
                     /translation="MKLAVLALLAVACGHSAKPTNPVAPPKPQTLAERALAVLPDGAQ
                     IVVELDLARLRANDVVGEVATRVFADVSIDAKLRLPGVTESANEPTAAAATSSPLAHA
                     DLLVLASYGVGTPQATTLTLLATKDDIAGATRLSPELVVLGPEEWVRQVEARAGIAAR
                     CPMEGAAAGTCSPLQGSEELLRMRDHAMPDAAPGAVLRVTALLSFDARVAMARQVGIE
                     LAPARLSVWADVVDDFAMVIDADAADPGDKTKKDAVERMRTGIRRLLAEAAAEPALRL
                     LGVTSAINDARFVEQKTWVRAIVAIGPRQLARVVERAKTLLPPSS"
     gene            complement(5731..6855)
                     /gene="hisC"
                     /locus_tag="VMZ53_30085"
     CDS             complement(5731..6855)
                     /gene="hisC"
                     /locus_tag="VMZ53_30085"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HUS32803.1"
                     /translation="MPTPPGSTESWLEVLAPAHVRGLDVYQPGKPIEELERELGISGA
                     VKVASNENPLGPSPKALAAIPKALSQLNLYPDAGGFALRRALSAKLGVPIEQLTLGNG
                     SNDLLYQLVLATCDATDEVLTHKFAFLSYRLSAQVAGRPFVAVQPTPELGCDANALIA
                     GITPKTKLVVIGSPNNPTGSVVTRADGEKILAALPERALLVIDEAYAEYAAQWPEVDH
                     LDGMALAKQDKRVVVLRTFSKIYGLAGLRIGYAIAAREVVDLVNRVGRTFHVSNLAMV
                     GAIAALDDQEHVEISAKHARTAIERLRSEVKAKVYPSLANFVLIDCGKPSTPIYDRLL
                     KMGVIVRPMAAWGLPNHIRVSVAAAPDMPRVIAALNEALA"
     gene            6919..8256
                     /locus_tag="VMZ53_30090"
     CDS             6919..8256
                     /locus_tag="VMZ53_30090"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HUS32804.1"
                     /translation="MRRIPLPWLAAALVLLVPAIAGADDGTFATYEERGYFWMFLAAF
                     GFGFLTSLTPCVYPMIPITLSIFGARGKDVSKRRAVALATMYVAGMGLTYSILGVTFA
                     LIGKAGDFGTQLANPWIVFPLVILFLALAASMFGAFDLNLPSSLQAKLNQVGGSGFGG
                     AFAMGLVGGLIAAPCTGPFLAGLLASVSTTGNVALGATLLFTYAIGMGVLFWVLAAFA
                     LSLPKSGRWMEGVKSAGGIGLLFAAIYYLKPFLPLGIRHAASPAWWFLLIVLAVAVIG
                     ILLGAVHLSFHGPWGERGRKAFAVAMVLAGLVGAWMWWMTPKHRLPWVYGDEQAAFDR
                     ARSEGKGVMVDFSATWCNPCDELEVTFGDDDVYGEITKNFVPLKFDVTKDSDKNDELK
                     LRYDSLTLPSVVFMSPDGKVLGRIRKFMEPEDALKVIGTSVKKLDAQRTAASK"
     gene            complement(8249..8869)
                     /locus_tag="VMZ53_30095"
     CDS             complement(8249..8869)
                     /locus_tag="VMZ53_30095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32805.1"
                     /translation="MGRFHYVVVLAALAVPATASAEVFKLYGEAQGGGLFGKGIAGDH
                     KEESAFQGGRGAYGVAAGAQFLIFDVNIRHNQFINGDGLTTWTQFTGGFNFGFDTGTE
                     QEKKENKGGYVGFGVGLAFGVGTGAQVDPPLDNSQVTDKGFLLEGKLNFGKHLNKVFD
                     IGLQVPVSYGYLFKSGNGATANNTETQYQSVQIEALLVLRANIRFI"
     gene            8909..9208
                     /locus_tag="VMZ53_30100"
     CDS             8909..9208
                     /locus_tag="VMZ53_30100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32806.1"
                     /translation="MKWLFVICVALLGACGDHEATKLAKIRDEVCHCKTAKCADEAMA
                     QLPTKDIQSTPRARRVAREMLDCLAELYNAQRPTDDPDADVSGPGTSDPASARTP"
     gene            complement(9162..9941)
                     /locus_tag="VMZ53_30105"
     CDS             complement(9162..9941)
                     /locus_tag="VMZ53_30105"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HUS32807.1"
                     /translation="MSELEVVDRGKVRTITLDRPESKNGLTHEFTLSIVQAITGATSA
                     NAIVLAGANGNFCSGLDLKDAMKRGLQPGPQLREQMSESFHGVIRALRTCGVPTIAAV
                     DGPAVGYGCDLALACDLRIVSERATFGEIFIKRGLMPDGGSTFLLPRIVGLGRALELF
                     YTGDVIDAKEAYRIGIANRVVPVAELDSTVAAMADRFAAGPPLAFEALKNAVYDNLDE
                     ASLSRALDREADGQMQLLASKDFAEGLTAFLQKRDPKFQGH"
     gene            complement(9938..>10249)
                     /locus_tag="VMZ53_30110"
     CDS             complement(9938..>10249)
                     /locus_tag="VMZ53_30110"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUS32808.1"
                     /translation="LTPKIVGHRVGLSESGALFAVVAGGKLLGFVGVVLAVPIAATVA
                     VLVRYAVRYYEHTEFFGRESDADVVITPAMAMIMPGIKIEAVPPAPEQHQDLQPTGAE
                     K"
BASE COUNT         1616 a         3367 c         3480 g         1786 t
ORIGIN      
        1 agaagtacat caaggatcgg tcgggcttta actgctccga cgcggtggcc gacatgctga
       61 gcgaccacgt gcgcgcggtc tgcgacgacg cgattcgcgc cgcggcgaag gacgagcgca
      121 agaccgtgct cgatcgcgac gtgccgcgac cgatccgtcg ctgagcgcga tcttaacggc
      181 gcctgccgcg cgccgtggcg agccagtagc catcgcgctc gagccacgac gcttcgatct
      241 cgacgggcag ccgcgtggca tcgagcacgg tgaccgagac ggagccgtcg gagcgcacgt
      301 cgagctcgat ctcggtgaag ccgacgtagc gacgcacgat cggatcgatc gctttgtaga
      361 tcgcctcttt gatcgagaag tagagcgtca catcgcgacc ggagacgtgc gcttgctcgc
      421 gcggcgtgag gatgcggcgc tcgatcggca tgcgcggcgg cgcggcgagc tcgatgtcga
      481 ggccgacgaa gccgcggccc gccggtgcga cgaggcctgc cgcgcgctcg gccttgtggc
      541 tgatcgagcc gacgaacccg gcgggcagga tcggcgcgcc gcgatcatcg gcgaggatcg
      601 gttgcgccga cagtgtgctc gcgtcgagct gtgacggcgc ggcgagctcc gcgagtgccg
      661 tgcgcaatgc ggtgcgacct gcagcgagct cgcgcttgcg cgcggggccg aggcttgcgg
      721 cgatgaggtg ctcgtcgccg acgagcggat ggtcgtcgcc gagcgtgacg atcacgcagc
      781 ggccgtgcgg cgtatctagg acatcacgat ttcgcacgag ctttgggctg catcaccatc
      841 aggtgattct tcttgtcctt gtcgggatag ccgaagcgga acgggagatc gcggtgcggc
      901 tcttcggccc agagcttgat gaattccttg cgcgcaccga tcggatcttg cgtgaagtac
      961 ggccccgcga agtcgccgta cgtgacgagc tcgtagccgg ccttctcggc gtagcgcagc
     1021 gggatgccgg acgcgtcgga gatcatccac tgcgtgttcg cgagcaggta atcgcggatc
     1081 tgcgagaagt cgtcccacca cagcaggaac gacgcggcct tcgtcatgac cgcgaccttg
     1141 cctttggacg cgaggtgttt gaggacgcgg tcgtcgtcct tcatgtgctt gtcgtcgagg
     1201 ttcgcgagga tgtggcggta cgtgcgaacg gcagccttcg tgtcgccacg cgggcggaat
     1261 tggatctcga cgttgccgaa cgccgagtgt tgctcgtacc agaagtgctc gacccacttc
     1321 ttcttcgtgc ggtacggccg gccgatcttc tccgcgcggt catccatctc tttcgcggtg
     1381 agatagtgga gcgtgccgtc tttctcgaga tcgaagtagc gcagctggac gggctccatg
     1441 tcgtgcaccg cgagccccgc gagcgcgaac atgatcgtgc cgggcaggat cgagtgcgac
     1501 gcttgctgca gcgactgcgt cgtcgagtgc gcggctttgt agaggcggtt gatgtccttg
     1561 ctgatcgcat cgagatccgc gtcgaggcgc ttttgatcga tcgagtcgac ggcgcgaacg
     1621 tcacccgccg cttcgagcga gatggtcgtg atctcggtgg cgtcgggata gaccgtgagc
     1681 gcgctcgaca gatcgccgcc gccaaacgga tacacgacgg tcgcgggcac atccgtcggg
     1741 cgcagcttcg cgatgaacgg cttggccttg tcgacccacg ccttcttgta gccggcgtag
     1801 atccgattca tgcgctggca atgcccggta atcgagtgct cggagaggcg ctcgggaacc
     1861 gcgtctgtcg tgttgccgca cgctgcgatc cggaacatca gcttcacctg tgcggcgtac
     1921 tcgtcaccct gcccgtcggc gtgcgcgagc cgagtcacca gcagcagcaa tccaaccacc
     1981 caaagccggc cgcgcatgtc ggcgttgtat cgcatgtccg acgtctgcca gccgatgtgt
     2041 tgagtctctc ccagtgagga cggccaagtc ctcgcaactg ctagccgacg caacgcggca
     2101 cagaggctgc tttatccgga tgcatgaagc gcctgttcgt cattgccgcc ttggtcgctg
     2161 ccgccgccac cggttgttcg gagccgcact ccgtcatggt caagcgcccg caggacccgc
     2221 aggtcgatgc cgccgtgacc gcgatgtggt cgcacgagat ccactcggtc gcgcaggacg
     2281 gcgactggat cgtgtcgcgc agctactacc tgctcgccga cggcctcacg atcgcgacga
     2341 ccggcgagcc gctctcgcac gcatcgatct acgacgcgaa gcgcgacacg atcatcgaat
     2401 cggttggctc gggcgtgcgc gagattccgc tctccgagct cgtggagcgc aaccactaca
     2461 tcatcgtgat ccatccgagc aacatgaccg cggtccagcg tcgccactcg gtcgagcgcg
     2521 cgcgcagccg catgggcacg ccgttcgaca tcggcggcat gttcggcttc gacgacaagg
     2581 acaaggtgta ctgctccgag ctcgtgtggt gggcgtcgca aggcgagctg cgcactggcg
     2641 tccactacat ggtcgttacg cccggcgacc tcctgaagca cggcgaggtc gtctactgga
     2701 gcggcaagcg caccgacgag caggtcatgt cgatcgcgat ggcgaacgag tcgcgcgacc
     2761 gcgccgagca gcttgcgaca caggagatcg ccgccgagca gctcgccccc gagcagctcg
     2821 ccgcgaagta atcacgcctc gctgcggatc gtggcgccca gctgagtgag tgccgcagca
     2881 aatcccggat acgacgtcgc gacgttgtcg gcatcaccga tgtgcgtcgt gccactcgca
     2941 acaaggccgg ccacggcccc ggccatcgcg atccgatgat cgccatccgc gtgcacgttc
     3001 cccggctcga gtgggccgtc cggccgcccc tcgacggtga agccatcggg atgcgcctcg
     3061 cagttgacgc cgaacgactt cagcatcgcg atcgtcgtcg caatgcggtc cgactccttc
     3121 acgcgcagct cctccgcatc gcgcacgcgc gtgacgcccc gcgcccgcgc cgcgatcacg
     3181 gcaaggatcg gcagctcgtc gatcgagcgc accgccaagt cgccggcaat ctccatcgcg
     3241 cgcagctcgc gcacgccgcc gcgcacgacg agtgtcgccg tcggctcggg cccgctgtca
     3301 tcgcgatctt cgagcgcgac ctcgccgccc atggccgcga tcgcgtcgag aaagccagtg
     3361 cgcgtcggat tgatgcagat gccaggcagc cgcacctcgc ccgcccccac cagcagcgcc
     3421 gcggcgacta gaaacgccga tgacgacgga tcgctgggca cctcgaagcc cgcgccggca
     3481 agtcgccgat cccaaccgcg tgtctcgatc gtcgtcgtgc gcccgttcac gcggagtggc
     3541 gctcccaaat acgcgagcat gcgctccgag tgatcgcgac tcgggcctgg ctcggtgacg
     3601 cgcgtctcgc catccgcata gagcccggca agtaacacgg ccgtcttcac ctgcgccgac
     3661 gccatcggca agacgtaatc gagcgcgcgc aacgctccgg tcgcgggccc gacgacgagt
     3721 ggcggcgtga catcgagccc accgccctgc gcagtgccgc cctgcccctt gatcgtcgcg
     3781 cccatgctca tgagtggctc gatcacgcga cgcatcggcc gcttgctgag cgactcatcg
     3841 ccgacgagcg tggtcgcaaa cctctgccca gacagcagcc cgcagagcat gcgaatcgtg
     3901 gtgcccgagt tgccgcaatc gacggcccca ctcggcgcgg tcatcccgtc gaggcccgta
     3961 ccttgaatga cgatggcctt cccatcgcgg ctcacgctcg cgccgagcgc gctgatcgct
     4021 ttcgccgatc gcccgttgtc cgcgccatcg ccgagcccca agacgcgaac cggcgtctgc
     4081 gaaagcagcg aaaacaggag cgcgcgatga ccgatcgact tgtcgcccgg cacgacgatc
     4141 gttccagaca gctggcgcgt agcaggctcg acgatgagct tcacgccgac atcttaaagg
     4201 cgtctgggaa acgtgcgtgc aaaggatgcc gagtcttttg cccgcgtcat ggcccagcat
     4261 tcatgcagca agtcggtccg gggccagggc gcgcacgcgt ctcgtcgctc atcgcgagtg
     4321 ttaccagcgc agcaatctat cgtgcttgat gacccgcgtt cgtgcccacg tgggcacgac
     4381 gaactcgtcg gtcttcgcgg cagcaatgcg cgtgcggagc cgcggcgtcg atgttcggcg
     4441 ttcgtgccca cgtgggcacg acgaactcgt tgggcttcgc ggcagcaatg cgcgtgcgag
     4501 cgcggcgccc acgtgggcac gagagaaccg gggtcctcgt ggtcgaggac acggcgcgtc
     4561 cggccgctcg tcgcggtctc gctgtctgac tcggcaggga gcgccgggcc acacgttttg
     4621 tcttcgcctc tgacgatcga cgttcgtgcc cacgtgggca cgacgaactc gtcgggcgct
     4681 cgcggccgca atgcgcgtgc ggagccgccg cgtcgatgtt cgacgttcgt gccacgtggg
     4741 cgcgacgcgc cacggcgctt cgtgccggct agctcgacgg gggcagcagc gtcttcgcgc
     4801 gctcgacgac acgtgcgagc tgacgggggc cgatcgcgac gatcgcgcgc acccacgtct
     4861 tctgctcgac gaaccgcgcg tcgttgatcg ctgatgtgac gcccaggagg cgcagcgcgg
     4921 gctcggcagc ggcttcggcc aggaggcgcc tgatgccggt gcgcatgcgc tcgaccgcgt
     4981 ccttcttcgt cttgtcgcct gggtcggctg cgtccgcgtc gatgaccatc gcgaaatcgt
     5041 cgacgacgtc ggcccacacg gagaggcgcg cgggcgctag ctcgatgccg acttgccgcg
     5101 ccatcgcgac gcgggcgtcg aacgagagca gcgcagtgac gcgcaggacc gcgccgggtg
     5161 ccgcgtcggg cattgcgtga tcgcgcatgc gcaacagctc ttcgcttcct tgaagtggcg
     5221 agcatgttcc cgcggctgcg ccttccatcg ggcagcgagc cgcgatgccc gcacgtgctt
     5281 cgacttggcg cacccactcc tcggggccga ggacgacgag ctcgggcgag aggcgtgtcg
     5341 cgcccgcgat gtcgtctttc gtggcgagca gcgtgagtgt ggtggcttgc ggcgtgccga
     5401 cgccatacga cgcgagcact aagaggtcgg cgtgtgcgag cggcgagctt gttgcggccg
     5461 cggcggtcgg ctcgttcgcg gattcagtga cgcccgggag ccgcagcttc gcgtcgatgc
     5521 tgacgtcggc gaacacgcgc gtcgccacct cgccgacgac gtcgttcgca cgcaggcgcg
     5581 cgaggtccag ctcgacgacg atctgcgcgc cgtcgggaag cacggcgagt gcgcgctcgg
     5641 cgagggtctg cggtttgggc ggcgcgacgg gattcgtggg cttcgcgcta tggccgcacg
     5701 cgacggcaag cagcgcgagg accgcgagct tcatgcgagc gcttcgttga gcgcagcgat
     5761 gacacgcggc atatcgggcg ccgcggcgac cgacacgcgg atgtgattcg gcaggcccca
     5821 cgcagccatc gggcggacga tcacgcccat cttgagaagg cggtcgtaga tcggcgtcga
     5881 tggcttgccg caatcgatga gcacgaagtt cgcgagcgac gggtacacct tcgctttgac
     5941 ctcgctgcgc agccgctcga tcgcggtacg cgcgtgcttc gcactgattt cgacgtgctc
     6001 ttgatcgtcg agcgcggcga tcgcgccgac catcgcgagg ttcgagacgt ggaacgtgcg
     6061 gcccacgcgg ttgacgagat cgacgacctc gcgcgcggcg atcgcgtaac cgatgcgaag
     6121 gcctgcgagg ccgtagattt tggagaacgt gcgcagcacg accacgcgct tgtcttgttt
     6181 cgcgagtgcc atgccgtcga ggtgatcgac ctcgggccac tgcgcggcgt actcggcgta
     6241 cgcctcgtcg atgacgagaa gcgcgcgctc gggtagcgcc gcgaggatct tttcgccgtc
     6301 tgcgcgcgtc acgaccgagc ccgtcgggtt gttcggcgac ccgatcacga cgagcttcgt
     6361 cttcggcgtg atgcccgcga tgagcgcgtt ggcatcgcag ccgagctcgg gcgtcggttg
     6421 gaccgcgaca aacgggcggc ccgccacctg cgccgagagg cgatacgaga ggaatgcgaa
     6481 cttgtgcgtg agcacttcgt cggtcgcgtc acacgtcgcg agcacgagtt ggtacagcag
     6541 gtcgttcgag ccgttcccga gcgtgagctg ctcgatcggc acgccgagct tggccgagag
     6601 tgcgcggcgc agcgcaaagc cgccggcgtc cgggtagagg ttcagctgcg agagggcttt
     6661 ggggattgct gcgagcgcct tcggcgacgg gccgagcggg ttctcgttcg acgcgacttt
     6721 gaccgcgccc gagatcccta gctcgcgctc gagctcctcg atcggcttgc cgggctggta
     6781 cacatcaagc ccgcggacat gagcaggcgc caggacttcg agccacgact cggtggaacc
     6841 agggggggtt ggcacggcgg ttcgcaccgt acaggcggaa tgccgcccga ggcaaccccg
     6901 gtctatagtg ctacccgcat gcgccgtatc ccgctgccct ggctcgccgc cgcgctcgtc
     6961 ttgttggtgc cggcgatcgc tggagccgac gacggcacgt tcgcgacgta cgaggagcgc
     7021 ggctatttct ggatgttttt ggcggcgttc ggcttcggct ttttgacgtc gctcacgccg
     7081 tgcgtctacc cgatgatccc gatcacgctc tcgatcttcg gtgcgcgtgg caaggacgtc
     7141 tcgaagaggc gcgcggtcgc gctagcgacc atgtacgtcg ccggcatggg gctcacctac
     7201 tccatcctcg gcgtgacgtt cgcactgatc ggcaaggccg gcgatttcgg gacgcagctc
     7261 gcgaatccgt ggatcgtgtt cccgcttgtc atcctgttcc tcgcgctcgc ggcgtcgatg
     7321 ttcggcgcgt tcgatctcaa tctgccgagc agcctgcagg cgaagctcaa ccaagtcggc
     7381 ggctcgggct ttggtggtgc gtttgcgatg gggctcgtcg gcggtctgat cgctgcgccg
     7441 tgcacgggcc cgttcctcgc tggcctgctc gcgtcggtct cgacgacggg caacgtcgcg
     7501 ctcggagcca cgctgctctt cacctacgcg atcggcatgg gcgtcctgtt ttgggtgctc
     7561 gccgcgtttg cactgtcgct ccccaagagc ggccgctgga tggaaggcgt caagagcgcc
     7621 ggcggtatcg gccttctgtt cgcggcgatt tattacttga agccgttctt gccgctcggc
     7681 attcgccacg ccgcatcgcc tgcgtggtgg ttcctgctga tcgtcctcgc cgtcgcagtg
     7741 atcggcattc tcctcggcgc cgtccatctc tcgtttcacg ggccgtgggg cgagcgtggt
     7801 cgtaaggcgt ttgccgtcgc gatggtgctc gcgggtctcg tcggcgcatg gatgtggtgg
     7861 atgacgccga agcatcgtct gccgtgggtc tacggtgacg agcaagccgc gttcgatcgc
     7921 gcgcgctccg agggcaaagg cgtgatggtc gatttctcgg cgacgtggtg caatccgtgc
     7981 gacgagctcg aggtcacgtt cggcgacgac gacgtctacg gcgagatcac gaagaacttc
     8041 gtcccgctga agttcgacgt cacgaaggac agcgacaaga acgacgagct gaagcttcgc
     8101 tacgactcgc tgacgctgcc gtcggtcgtg ttcatgagcc ccgacggcaa ggtgctcggg
     8161 cgcatccgca agttcatgga gcccgaggac gcgctcaaag tcatcggcac ttcggtgaag
     8221 aagctcgacg cgcagcgcac cgccgcgtct aaatgaagcg gatgttcgcg cgcagcacga
     8281 gcagcgcttc gatctgcacg ctctggtact gcgtctcggt gttgttggcc gtcgcgccgt
     8341 tgccactctt gaagaggtag ccgtacgaga ccgggacttg gagcccgata tcgaagacct
     8401 tgttcaggtg cttgccgaag ttgagcttgc cctcgaggag gaagcccttg tcggtgacct
     8461 gactgttgtc gagcggcgga tcgacctgcg cgccggtgcc gacgccgaag gccaggccga
     8521 cgccgaaccc gacgtagccg cccttgttct ccttcttctc ctgctcggtg cccgtgtcga
     8581 agccgaagtt gaagccgccc gtgaactgcg tccacgtggt gaggccgtcg ccgttgatga
     8641 actgattgtg gcggatgttg acgtcgaaga tcaggaactg ggcacccgcc gccacgccgt
     8701 aggcgccgcg accaccttgg aacgcggact cctccttgtg gtcgccggcg atgcccttgc
     8761 cgaacagacc gccgccttgt gcctcgccat agagcttgaa gacctcggcc gaggcggtgg
     8821 ccggcacggc gagcgcagca agcacgacca cgtagtggaa tcgacccatc gctcgattct
     8881 ggcacagcgt gcttgtagag tgactcagat gaagtggctg ttcgtgatct gcgtcgccct
     8941 gctgggcgcg tgcggcgatc acgaggccac caagctcgcg aagattcgcg atgaggtttg
     9001 tcactgtaag acggccaagt gcgcggacga agcgatggcg cagctgccga cgaaggacat
     9061 ccagtcgacg ccgcgagcgc ggagggtcgc gcgcgagatg ctcgactgcc tggccgagct
     9121 ctacaacgcg cagcggccga ccgacgatcc ggacgccgac gtcagtggcc ctggaacttc
     9181 ggatcccgct tctgcaagaa cgccgtaagg ccttccgcga agtctttgct cgcgagcagc
     9241 tgcatctgac cgtcggcctc gcggtcgagc gctcgcgaca gcgatgcttc gtcgaggttg
     9301 tcgtagaccg cgttcttgag cgcttcgaac gcgagcggcg ggcccgcggc gaaccgatcg
     9361 gccatggcgg cgaccgtcga gtcgagctcc gcgaccggca cgacgcgatt tgcgatgccg
     9421 atgcggtagg cctctttagc gtcgatcacg tcgcccgtgt agaacagctc gagcgcgcgg
     9481 ccgagaccga cgatccgcgg aagcaggaac gtgctgccac cgtcgggcat gaggccacgc
     9541 ttgatgaaga tctcgccgaa cgtcgcgcgc tccgagacga tgcggaggtc gcacgcaagc
     9601 gcgaggtcgc agccgtagcc gacggctggc ccgtcgacgg cggcgatcgt cggtacgcca
     9661 catgtgcgga gtgcgcggat cacgccgtga aacgattcgc tcatctgctc gcgcagctgc
     9721 gggccgggtt gcaggccgcg cttcatcgca tctttgagat cgagccccga gcagaagttg
     9781 ccgtttgcgc cggcaagcac gatggcgttc gcgctcgtcg caccggtgat cgcttgcacg
     9841 atcgacagcg tgaattcgtg cgtcagcccg ttcttcgact cggggcgatc gagcgtgatc
     9901 gtgcggacct tgccgcgatc gacgacctcg agctcactca tttctcggct cccgtcggct
     9961 ggagatcctg gtgctgctct ggggcgggcg ggaccgcctc gatcttgata cccggcatga
    10021 tcatcgccat cgcaggcgtg atgacgacat cggcgtcgga ctcgcggccg aagaactcgg
    10081 tgtgctcgta gtagcgaacc gcgtagcgca cgaggacggc gaccgtcgct gcgatcggca
    10141 ccgcgagcac cacaccgaca aagcccagca gcttgccgcc cgcgacgacg gcgaacagcg
    10201 cgcccgattc ggagaggccg acgcggtggc cgacgatctt cggcgtgag
//
LOCUS       DATLBZ010000615        30284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7889004, whole genome shotgun sequence.
ACCESSION   DATLBZ010000615 DATLBZ010000000
VERSION     DATLBZ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 30284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30284
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7889004"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VMZ53_30115"
     CDS             complement(<1..424)
                     /locus_tag="VMZ53_30115"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUS32809.1"
                     /translation="MTDALLALTLVAVTALVTVFIMRRRIARLAAAAHDVVRNQARRV
                     PTASTTGELRSLAEWINALAEDAERSRNALHHERTLLGAVADLLTHGVIAIDGERKIE
                     MLNDAARRMLGVQSSLVGESLLEFIRVPELHALVERTED"
     gene            complement(421..1128)
                     /locus_tag="VMZ53_30120"
     CDS             complement(421..1128)
                     /locus_tag="VMZ53_30120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUS32810.1"
                     /translation="MSGLVLVIEDEVDLATTLEYNLRAEGFEVRVAHNGRQGIAAAST
                     DPLPDVIVLDLMLPDLSGTEICRRIREQDRTREIPVIMCTAKGEEIDRVVGFEVGADD
                     YVVKPYSVRELILRIRAQLRHRTQKPDGEPSLIKFGRLELDHDAHRAWVDKTEIALTA
                     LEFRLLHAFITRKGRVQSRDALLSDVWGIDADVTTRTVDTHVKRLREKLGEAGMYIET
                     LRGVGYRFKSDPDEADA"
     gene            1243..2292
                     /locus_tag="VMZ53_30125"
     CDS             1243..2292
                     /locus_tag="VMZ53_30125"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HUS32811.1"
                     /translation="MGPGGLEVWQWLGLGVVLVVSVLLGRIGAAVLTWLGGKLAGKTT
                     TRLDDDLVVKLRSPLRFLVGTGVARIGMLPLELPPHPTQIVIEVLQAAFAFGLVWGAL
                     RAIDVMAGHLATAQWAATRPSSRSLLSLISRVAKVLVIVIAGIGFLSGIGLPVASLLA
                     GLGIGGIALAFGAQKTVENLFGAVAIGVDQPFREGDFVKVEADTMGTIEVVGLRSTRI
                     RTLERTVISLPNGRLADMRIETFAHRDRIRFATVLNLVYGTSAGAMREVLAELEKVLS
                     DHTGTVKDEITVRFLGFGQSSLDVEVICYFMTADMPQFRAWRQEVLLAFMDIVERKSG
                     FAFPTRTVHMLQSRP"
     gene            2292..2678
                     /gene="yhbY"
                     /locus_tag="VMZ53_30130"
     CDS             2292..2678
                     /gene="yhbY"
                     /locus_tag="VMZ53_30130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HUS32812.1"
                     /translation="MLTGKQRRHLRALAHDMKPIVQIGKGGIDGGLISALDQALSDHE
                     LVKVKVGENAGVDRHEAAENLATATKSQVAQVLGNMLLLYRPDPDDPQIELPRAKAAA
                     GAAASTDADDAEADADDDDDDDDDDE"
     gene            2638..3153
                     /locus_tag="VMZ53_30135"
     CDS             2638..3153
                     /locus_tag="VMZ53_30135"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS32813.1"
                     /translation="MLTTTTTTMTMTNSATALAVLIAVAACGSAAAPTESRAPVAACG
                     APAGGNEQITKAVLDMYAALRTDDLAGFRAVTEPSFYAFEGGERMDGDALVDAIKNAH
                     AQGKRFEWTVTQPVVVVDCTIAHVTYVNVGAIGDASSMQPRSWVESALLRYRGDRWRI
                     QFFHSTRVPAK"
     gene            3171..4517
                     /locus_tag="VMZ53_30140"
     CDS             3171..4517
                     /locus_tag="VMZ53_30140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32814.1"
                     /translation="MSRIGLVLAALCVVTATAWAGDPNRWQAWVSPTDGESNAVDVSM
                     VSGDRVACGKKPWIQLQPRFRNRYQERITGHLKIEYIDVDGNGQVATPEFDPNAGETK
                     IMALAAFCHDPRQSLKFSIADMKFPAREAEKARLEAQRLAAEKAAAEEAARKKAEKDK
                     AAAEQAAIEAAKREEAKKEAERLAKEGESSRKQTAAEIRAGQLESEESRNAAREAEAY
                     RQKRQLEAETEANRNVRVDELAADTFRNGEIIFGAPIGFQNTKFGAETEGRFTVGGRV
                     QLRLYKWLKRLSARGEPLSGSGLELTLEGGYATTIDIGENSSGAHFRVSGGHAHARYW
                     HGSLGIGAFGEWTHYWYDPPGNPNGTYDLYALGPELAFGIVASRSLALELALRGGAVA
                     SGSGLAFDTSTDLFVAASALAELNYLYGGAIATRYATSSTVDSWNALAVLGVRLPF"
     gene            complement(4514..5692)
                     /locus_tag="VMZ53_30145"
     CDS             complement(4514..5692)
                     /locus_tag="VMZ53_30145"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS32815.1"
                     /translation="MTLDPYARDLQMYSIVGEVNPSGRILARQVIPSPPTTSGTTSTA
                     LAQSKIVYLNKNGVTLQPGDNDSRTNKSTIVTQAVSLPAWNVTATNWQQTVSCMKDLF
                     SRFDVTITDVDPGNVPHMEAVFGGSPTQVGMDANTAGVSPFTLDCVVIENSIVFTFTG
                     AFQMTPREACEVMAQEVAHSYGLDHQLLASDPMTYLDYTGNRAFQDQTSQCGEYSARQ
                     CGINGSVCRTNQNSVSLLKERLGTKGGDAIAPQMSWTSPANNATVPPGFEVHAQGSDN
                     IAVKGAAIKIDGMQTDSKTGAGPFVFTTPMTLAEGVHTVVIEISDGINVKSETRTYTV
                     KKGAAPPSDPGSGGTGEGTGDPLDSGDIVGGCAAGGGQTGALLALGVLGLVVSRRRRR
                     "
     gene            complement(5944..7251)
                     /gene="pepP"
                     /locus_tag="VMZ53_30150"
     CDS             complement(5944..7251)
                     /gene="pepP"
                     /locus_tag="VMZ53_30150"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HUS32816.1"
                     /translation="MFDPSVFAARRDAYMQAIGPDAVAIVRSLPERLRNGDSFFPFRQ
                     ISDLLYLTGFAEPDTTLVLRPGAEKERVVMFVRPRDPEMEVWDGRRAGIDGAKQQYGA
                     DAAYPSSELPSKIGELISNCEELHFSVGLDDEMDLMITHAVAKLRKTEKRGKRPPKAI
                     IDPRVALHELRLHKRPEELVALRKASQISTDAHIAAMKAGKPGAFEHEIEALVDYTFR
                     KQGGTGPGYATIVGAGENATILHYIENRCAVADGDLVLVDAGCEYNGYTADITRTWPA
                     NGTFTEVQRRVYQIVLDCQVEAVEMVKPGATLDQIHELCVRRLTEGMVALGLLTGNVD
                     DLIKDQTYRKFYMHGTSHWLGMDVHDVGSYTHKGKARPLEPGMVITVEPGLYIAADAP
                     DVPAELRGIGVRIEDDVVVTATGNEVLTAGCPKTIKDIEAACR"
     gene            complement(7273..7953)
                     /locus_tag="VMZ53_30155"
     CDS             complement(7273..7953)
                     /locus_tag="VMZ53_30155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32817.1"
                     /translation="MQATYGIGGHGLLLAPSPAVRVMATADRELVLLMGEREVARITP
                     GDVPATGDAVWFASGIARFGDGGPMLEIEDGVELEAVFLKIDTGLPGIEWSAIAAGVM
                     VELPVDVVLVPAQPGDEDPYFELHAPGGIDEFISFRPRPVSAEEVVIKPAPYQRLVSQ
                     GITPDEIPFTECAYEHDGRQWRQIFYAVPLANDETVVVCAQAAEPHVELLFQAAEVAA
                     TSLVPMRS"
     gene            complement(8036..8614)
                     /locus_tag="VMZ53_30160"
     CDS             complement(8036..8614)
                     /locus_tag="VMZ53_30160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32818.1"
                     /translation="MRLLVVAIMFVVGCSKKTDAPATGSAASSGSSAGSGSAAVATNA
                     GSSVAIDAAGSAGAEAEKRPLPEWAVGKPAAIDDEAVAVVEELSAKLEVKRADLLSED
                     CAVAAAAMKEFIPLSVRLGNASDKLDAVSRGNVELVTWAKTYYIPRLQQVLNEVGAVI
                     QQTKCKHDPDYKAAALEYTKSNTGEVPVKKPE"
     gene            complement(8637..10253)
                     /locus_tag="VMZ53_30165"
     CDS             complement(8637..10253)
                     /locus_tag="VMZ53_30165"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS32819.1"
                     /translation="MKRLLLLVSIAGCAGQADSASPGGGNVSFGGQQDIGEFRGILEA
                     GQIPGPQTLDANGFFNEHYVAPPTDCTTTLCITPGVSVGRDWLTGAHQATLQIAVNTN
                     VDPTTITRKPLNLVVVVDHSGSMWDDQRLEKVKSGLHTLVQNLGEGDRLALVQFDDRV
                     DVLVPFAETVNKTQLDQVINNLTPRGSTNLYDGLEQGFKLAATAFSSDRQNRVVFLSD
                     GMATAGNTSQTDIIAMADRYVTDGIGLTTIGVGLSFDVSLMRGLAEHGAGNFYFLEDA
                     AAASEVFNQELDYFVTPLALDVHVNATSGSGWQFGEVIGSTLWKSQTTAGSMAIPAVF
                     VASRTSQGPEGGRRGGGSMLFIHLTPTGQNGPDGKVADLTLTYRLPGSSEIQTQTITL
                     AYPNEPSEIPADTYLSTPEMAERYAMYNIFLGLRMATNAYDYNCAASTLNAVKTSAQT
                     WNSTHEDPDIAADIQLIDLYLTNLRTYGGTGDTASGTCASDPYGDDYGDGYYGDDTYN
                     GACMSAGGNSRGVVLIVLAVAFVVRRRRRA"
     gene            10370..12181
                     /gene="ggt"
                     /locus_tag="VMZ53_30170"
     CDS             10370..12181
                     /gene="ggt"
                     /locus_tag="VMZ53_30170"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HUS32820.1"
                     /translation="MRRLLVLGLSLVFAACSSQPPKMVVPAPAPPPPAEMPAPPRGVT
                     DAAVAIAPDAGPPPLPFRTGASGFNVDPNVAGTGKTYMVATEDEYATKAGVAVLASGG
                     NAVDAAVASAFVLAVTLPSAGNIGGGGFAVVRSGKNKIAALDFRETAPAAASPDMYKD
                     DPKSSLAGYRASGVPGSVAGLEALHKKYGKKKWKELLAPAIKLARDGYVINAHVNLSI
                     VLGNRVAPMSGNLAAIYLPNGKAVETGATVKNPELADALQLIADKGSAGFYKGTTAKA
                     IATAMKANGGLITEKDLAGYKVVWREPLKYTYRGKTLYTMPLPSSGGMVLALTANMLR
                     GIDLAKLGWHSTQHVHWLTEVWRRGYAARNLALGDPAYVKDMPIARLTSQAEADRLVA
                     TITERATPSKDVPPLIEGKHTTHFNVVDAKGMAVALTTTLNTGFGSGVMINGIIMNNE
                     MDDFATIPGQPNAYGLVQGEANKIEPGKRMLSSMSPTIIEDDKGELYMVVGAEGGSRI
                     ITAVWQTLSNVIDYGMSIDQAIAAPRIHHQHLPDDVVFDDEAITADVDAELDKLGYVR
                     VWNQRSRIFSSATGIVKTPAGWAGAADPRRGGAALGD"
     gene            complement(12188..13822)
                     /locus_tag="VMZ53_30175"
     CDS             complement(12188..13822)
                     /locus_tag="VMZ53_30175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32821.1"
                     /translation="MRLTNHTAVAVLALVGCGVDAVDDSTATLRVEPADVSQVVTDGQ
                     PVSTQYRALLVTADGSERDVTAESTFALARPDLGTWQSSSLELRGAAVGPSAITATFE
                     GMTASTNLTVYAHTRIVEEDVVNMPAWFDALRSDETCFPFVMYPEGGTILPSNLDGID
                     AQWADFANDRFEVSFATQYATVELYTRNHERALSADAWAQLASTHEPIMFHVSSLSSG
                     SPTKVCTSQDRRLLVSDKPLTGAIYTWSDGGGIWRQDAATRGEPEPMIMPGTGNITQM
                     QTAPLVTSCSGCAVSRSGARMALRLDSGIGTILDFTQDRVMQPTSWSSATFTRNADKL
                     VIAQDGILKLIDETGGELATLVNTPNKTAMDPQFSPDGSKLVSVESSGFSSSGTGNLV
                     VRTFMDDGNVFGNPAQLVGGGPGVANYSPSWSPDGKWLAYTRTSGWGTSSPLTSIWVI
                     KTDGTEQPIQLVAPTSDVDMAARWAPGAHSLNGEAFYYLTFDSQREYGANDIGRQIWM
                     MAFFPQTGFARPAFHVPFQSLETSNHVAQWADALVR"
     gene            13903..14805
                     /locus_tag="VMZ53_30180"
     CDS             13903..14805
                     /locus_tag="VMZ53_30180"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS32822.1"
                     /translation="MAEKNDPSGIAVYPKLPVPKSRTSEPKVKGFKDNDGFSIPKVVA
                     ALVIAAAVGGGVGFLLAPSKAADLKKAKTDLAAAQTAMKVEKDRADGAEQRAVVLEKD
                     KTELAQKVTELSAKAGEMEKKVADANAAAEKKLSAAIDKSQGTVSTEGDEIHLKLVDK
                     VLFAVGDDQLTDKGKAALDKVAKALAELPDKQVWVQGHTDDTPIVIPPPPKPDPKKKK
                     GAKPEAPVAPMVRFASNWELSAARALQVVHYLQDHGKIDPTRLAALAFSEYRPVSKSN
                     KAQNRRIEIVLYPHKAVIEKATKK"
     gene            complement(14806..15582)
                     /locus_tag="VMZ53_30185"
     CDS             complement(14806..15582)
                     /locus_tag="VMZ53_30185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS32823.1"
                     /translation="MSGAVARGGRILIVDDEPMVRDTLGQVLVDEGYVVDTAVDGEDA
                     LARVKAARPDAILLDLMMPGMNGRQFLQALRKDPSYEQVPVLIMTAVHGLEVNLASIG
                     ASEVVEKPFDADDLLNKVALAVYRSRDADRVAHSPFIAAPSEPTPEPSDRGVILLVEH
                     DRARLQRLDVVLSERGYTVVSMTRAPAQLARLARALRPRAILLELASEGVADVAKEIR
                     DSSEPLPLLLFSRDDAGGVELAHAADSDLLSFVDKCTTAR"
     gene            15639..16109
                     /locus_tag="VMZ53_30190"
     CDS             15639..16109
                     /locus_tag="VMZ53_30190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32824.1"
                     /translation="MSTAPPVARAYDLLRPKILQRLLELRTTLDPSLGRLDESTARAQ
                     IGAVLDHLGNFIATGDLGLHRAFLHTFLAMRAAEAQGPAAVLAMLVAIGDTAAQVSQE
                     ENPNPEGSELTLLLTRVTASTARAINDLIAEELERRMAQWAELSTKEREGLEPA"
     gene            16106..17953
                     /locus_tag="VMZ53_30195"
     CDS             16106..17953
                     /locus_tag="VMZ53_30195"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HUS32825.1"
                     /translation="MTSQPQQPGLRIAGDTHVGSVRTTNEDSMIVDHQRGLYVVLDGM
                     GGANAGDIASQTARDAIATFVQQRRLNMEPKQLLESAIQYGCATVFNAAQQARERHGM
                     GTTCVACLVIDTKRAIVAHVGDSRAYLLRYGRLQALTRDHTIVEELVERGVLAADEAE
                     RHPYKNVLSRNLGARPETRVDVLELEIKPGDRIMLCSDGLYGYASNDGMQYLLGSGDA
                     PETVAHDLIELALRGGGGDNVSVIVIEAPQPAPSSTQVVRTSGATAWWQHRQRFLQIA
                     KDRGITKNPICRGLKPEEALDLVAASLCEAIFHDLEKSTGVNVWTFAQNVAGGWFERG
                     GEWKPLRDLMDILAISARTVVEDVRKADAKLGFLLDVAVSRALVVAELALGGLLAERL
                     RHVDQDLIQIHGAMQDTIDGDVERADTDSRFTDQATIPFLRPDRPVTNPGMSAELVTA
                     IEKTLTIARARTSPRAELVKQILIALETISTDPSGNFASAVLAARELYGVRSVDDSGI
                     MPLFDALDAARIITASSVHQISAPDAMRAKVLRAISSAHQRLVGAMTGLVLEAVAPYS
                     QRLEETKAVTGELRDKVARAERKRAELERKFASTIDQSLPWERRGTTEW"
     gene            17953..18462
                     /locus_tag="VMZ53_30200"
     CDS             17953..18462
                     /locus_tag="VMZ53_30200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32826.1"
                     /translation="MAEPEVDLIEAVKVLRDEVLETVEHGDRDPPGAEVFDALINALT
                     VGGESVPGLDLQLHDSVTRRLAWGDSEEVVLADAERVFDRLLFAAERAFREPADRMVV
                     IEAATQVAVTVARVVSLAAVGRASRDRAARLREEMAQRQLKDVLEKQRSSIVRLEFET
                     TTDRAPSEK"
     gene            complement(18463..19143)
                     /locus_tag="VMZ53_30205"
     CDS             complement(18463..19143)
                     /locus_tag="VMZ53_30205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HUS32827.1"
                     /translation="MSRRAPRYRISLNVTVMPLLGGKMAVCHTRDVSDVGICLDTSEV
                     SEWLPVGTRVSLALTDPRSGTAMEVIGDVVREVAGSNPALGVLLIEPPPEWKVLVTNA
                     ARQSGTVEKPGRRMRVLVVGDDHRQRGAMALYVSSGWDVLFASDDFAVHEALENIKLD
                     AVIAELDAADPRIQPIMDDVRKQQPAARRIVRGLGRSDDDLVHRYVDRDSGLDALLDA
                     VTANIAAP"
     gene            19250..19909
                     /locus_tag="VMZ53_30210"
     CDS             19250..19909
                     /locus_tag="VMZ53_30210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32828.1"
                     /translation="MRALLACAAMLVATRAYADEPQPTPTGPTLPTSPAPAPAPGPGA
                     ETAPSQPATPEPKADPAYGEKPDPAYGPPPGSTVPESSGPSAGRDIYIKSYPDRSHKN
                     VMGLSIAGGAAVVLGGVGLYFHLDSRSKSNDVSANKYTGEVWTAERQDTYDSANRSAV
                     VAGVFYGIGGAVLIGTAIAYMITEPKMETTVLHPHTSPKPTAMVAPTRGGALVGGAWS
                     F"
     gene            19909..20520
                     /locus_tag="VMZ53_30215"
     CDS             19909..20520
                     /locus_tag="VMZ53_30215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32829.1"
                     /translation="MRAAVLLIALAACTPDVFSESYLCGPDALCPEGQACNGPDNICV
                     LKGAAQPFSCTPKIATEPDDSAANAHEIANLLCPSLPFSDENCMLQDDAEDWVKLKAP
                     AMCNTNAVSTVKLAAGVSFPIAFERIAIELWDLGTMTKLADDAECTRGEGVVGEEIRC
                     LDYALVAGSSYGIKVRPAGAGDGDCDGNCRFNRYTLRVELSTP"
     gene            20530..22605
                     /locus_tag="VMZ53_30220"
     CDS             20530..22605
                     /locus_tag="VMZ53_30220"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HUS32830.1"
                     /translation="MTELRWSWQATPYLVCTLLLVAVTLGAALIRGDRVLRIGVIAAA
                     MTAIPWALCQALAACTDDAIVAARFLRLGQGPVASVGPNLMLVILGVSGQLERYRWVA
                     RLAGLVATVALVICWATDWVVPGAQRVPLGMYYMKPGPLTGLHISQLLIWLGVGLFIA
                     RRAAPGGSERKRLLRLLLGVLVAGAIGSIDTLLLYQIAGIYPIAWLPASVACGIALYL
                     IFKTDLLRPQGFDRGMAIEIAMFGATTIAIAALALALGTSHKIPLAAFSALAWAIATG
                     IAWSLIRARPVRVKGERELEQFIGRVTTYDDQKKIAERLGALWKRAVGIDVKAMWWVE
                     GAALTNSSGEKWTIDREVSDWLVQHDDPLALTDLATMRLGDLRAKVEALGAAHGATML
                     VPLVDRGELVGLIEADYSKALRDAERGLIDESARAAARSLTFVGLARAAARERETARE
                     VEVADALRLQASASRDAELGRWAVAAEYRTAARTTGAGWSAIELPDGRLALLVTEAQA
                     QGVAAALATAALTGAFAAATSGSGTVTRDGLISTMRASSEGVLRSGQPVSAFLAILDA
                     QAQTIEWACAGHPGGFLVGPIAAVDAGLPAGSTKGARPKATPITAGKRVKDASLTAAI
                     RGNSAFHPDTLLVVASTGLRGPDDESWQTRLQEFAPASGRLASVLVDTALKAGEPAED
                     MLAVVVRAR"
     gene            22696..24144
                     /locus_tag="VMZ53_30225"
     CDS             22696..24144
                     /locus_tag="VMZ53_30225"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS32831.1"
                     /translation="MPDAPADAPDTAAPMIIDSHPAKDGKGASVIQPITFVLDEAVDP
                     ATVTTATIKVRAWMKGALTDLLFSGGPVENAMAIEGTATYEAATHKIIFTPVVPLPFN
                     ARVEVTIAGVKDTAGNALATNVAFDTIRNMEAAQIFYATGNAISQYYDFAVDSMGMQR
                     KFSRVPGPGPDGSFFTADDPVTQHFDMVFDMNGRFIVSHSFQAGTDGMFYTSDDVETA
                     ALTSTYDMSGRLTHLVSNNAAGPDGMWGTADDVPNSMYVQSWMGDNSLGVIFFSSPGL
                     DGVWRTADDQMGSPTTQFTKYEYDSMGRRTRQVVNVPGADRVPGTADDTKQRYFDLTN
                     DSHGFVTRSVERTAGSNGIFFDADDTNIAYTQTTRDARGLATEIKLYDTAGVNGIWFD
                     ADDHVSVGSGTRRTFTYNMAGQVVEEVSWLAGSDGIMGTADDVISTYLTTVYDSKGAR
                     VSYTSTTGQGPDALWRTSDDQLGFRRTYDALH"
     gene            complement(24141..24380)
                     /locus_tag="VMZ53_30230"
     CDS             complement(24141..24380)
                     /locus_tag="VMZ53_30230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32832.1"
                     /translation="MHDTSFDDTLLVPRLPAEDPPARALPTTFAQARPRHWPWVVLVL
                     ALLALAALRPVNFSASQQAVERSQQADQLLPQLGR"
     gene            24627..26123
                     /locus_tag="VMZ53_30235"
     CDS             24627..26123
                     /locus_tag="VMZ53_30235"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32833.1"
                     /translation="MKRALALVVLVACGDNSVPPAAEEMVVPGCTDIPQPGPDPFPTS
                     ESVDAVAQGPVVPGTLAHWNLDGRWFLTGVRVGGISSYHFERSGDVVIVDRDNGHPAT
                     FDDDVLFQRSVLHSNGMDFVVIKRVSNRHADGSLRAERILCNGDGCRVCTAKMIRAER
                     NAGESEGDHISFVGELEDPAWPDSIALNVRVVDNLAYLVRRDGVHIIDVSDPAHPTSI
                     GAWGRPSPSGSSNDVKIVQAGPKRYVLTGDVPVDIIDVTDPAHPQLAGQIAEEAHTLA
                     VETRDNKVYAYFGNYDGKCPIYDVTDPTQPQKLGSFTTRGDLVHDLSIENGIAYLNAW
                     NDGFYVVDYTTPSAPTLVGHWAGTPAGSSHSSWPMTVGGRHIALHGDENFNAHLDVVD
                     VDPSSPQFMTSIGSYQTRDFISIHNLMAFGSKAYFTYYQDGVRVLEMADPTQPRLVGY
                     FNTWDPNGEFSSNGFFEGAVGLDVDVARKLIFVADIPRGLVILKDSTP"
     gene            complement(26098..28869)
                     /locus_tag="VMZ53_30240"
     CDS             complement(26098..28869)
                     /locus_tag="VMZ53_30240"
                     /inference="COORDINATES: protein motif:HMM:NF023945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUS32834.1"
                     /translation="MPQLVAPGEVAPRRQLPSFVYLAGDIDLAPHETALPWRPAPAKS
                     VDPQAAVSTALRPVVGELARSQGARMASRMIASAKSWLCHPGVDRGAAILPWGDNDGP
                     KLSPINAQAQVLRHIREAWDDEHADAPFEEQEVLVTVPASFDEAARELTLQASVAAGF
                     PPVVLLEEPQAAFYAWIDTARSKLAPGEHVLVFDVGGGTTDFTLIDVDPGGDGFTRTA
                     VGDHLLLGGDNLDLTLAKIVEQRVKDTSGKKLDALQWHGLVHACRLAKEKLLGDDPPD
                     TAPIVVQSRGAKLIGGTIRDEVSREELEKILFDGFFPFVAKDAQLSRGRSGLQEFGLP
                     YASDPAVTRHLAVFLQRHNATQIDAVLFNGGAMTPQTLRDRVLKQIGDWQGSPPRELE
                     AQMPEMAVAQGAAYYGLVRRGLATRIKGGTPRAFYIGVDATGSRRRVPTMEAGKSEEP
                     ARFAVCLAPPGLDDGARVQLARDFKLVTNRPVSFRLYSSSTREDQPGALVPIGDGKAE
                     TIDDGSDLLELPPIVTVLRARGRGEVTVRLEVHITALGALEIYCVDTEPPNETWKLAF
                     DMRSGGAAPAPEDAADAAPHPKAEEAKAKVKAAFSTGAGLQTLMRDLETLLEGRRDEW
                     SMQTTRALFDALIDGAADRKKTADHEQRWLNLAGFLLRPGTGAPLDAWRSRVMWGVFN
                     ENLAHAKSEPGKLAWWIAWRRIAGGLAKGQQDQIYDRTAQLLLPSTKQAKKLAETKPS
                     KQELAEMWRCVASMERLPIQHKVKLGDELVQRMATRKGREETVHLWALSRIGARVPLY
                     GPLNAVVPPNKVKEWLAPLMQGEWPDKADDPNGVSPVAFPLAQLGRRTGDRTRDLDDA
                     TRAQLASFLRNLRGGDRAVVLVEQVVALEAREERVALGDTLPAGLKLVIDDERPDSDG
                     EATASNPSG"
     gene            28994..29665
                     /locus_tag="VMZ53_30245"
     CDS             28994..29665
                     /locus_tag="VMZ53_30245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32835.1"
                     /translation="MQRRMKAGAVVVVSLLLAVGRAYASDPGNSCEDEASELRQALTR
                     ESSRAETWNTVWRWTFTGAAVSSAAIAYVDFDFLHDSQAGIYVSAGKASIGAIARWIM
                     PLHIHVPPPTADACADVEALHKEITRVAKKERGLFITGHIGGLLLNGVGGYVVYHYSG
                     LGQAALSVGLGYPIGLLSNYTMPRSSWHRYRDREWDLPPPAPMPTLSVIPTRGGWFVS
                     LGGAF"
     gene            29665..>30284
                     /locus_tag="VMZ53_30250"
     CDS             29665..>30284
                     /locus_tag="VMZ53_30250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32836.1"
                     /translation="MHRLALLAILAACGQPSRPTTSSPSQVASAPTPYSFDFLPADLR
                     ERGPSLIDIQCPAGTKRVETSSYGRTHRHCETPTKEYEGPIVEFAEGEIRLRGTHAGY
                     RSGIEISANAGGVIEAEYAANHPVGRYRYRKGSIVVAEGAFDSEGRLDGEWIFRTPVS
                     GQELTRTTFTHGTGSIEIWDWRGSQPKVAARLQCKDGLLDGAQMFHL"
BASE COUNT         5213 a         9928 c        10023 g         5120 t
ORIGIN      
        1 catcttccgt tcgctcgacg agcgcatgca gctctggaac gcggatgaac tcgagcagcg
       61 attctccgac gagcgagctc tggacgccga gcatccggcg cgctgcatcg ttgagcatct
      121 cgatcttgcg ctcgccgtcg atcgcgatca cgccgtgcgt caatagatcc gcgaccgctc
      181 cgagcagcgt ccgttcgtga tggagcgcgt tgcggcttcg ctcggcgtcc tcggcgagag
      241 cgttgatcca ttcggcgagg ctgcggagct cgccggtcgt cgatgcggtc ggaacgcgac
      301 gcgcttgatt gcgaacgaca tcgtgcgccg cggctgcgag ccgggctatg cgccggcgca
      361 tgatgaacac ggtgacgagc gcggtaactg cgacgagcgt gagcgcgagc agagcgtcgg
      421 tcacgcgtcc gcctcgtcag gatcgctctt gaatcgatag ccgacgccgc gcagcgtctc
      481 gatatacatg ccggcctcgc cgagcttttc gcgtaggcgc ttgacgtgcg tgtccacggt
      541 gcgcgtcgtg acatccgcat cgatgcccca gacgtcggag agcagtgcgt cgcgcgactg
      601 cacgcggcct ttgcgcgtga tgaacgcatg caggaggcga aactcgagcg cggtcagcgc
      661 gatctcggtc ttgtcgaccc aggcgcgatg cgcgtcgtga tcgagctcga ggcggccgaa
      721 cttgatgagc gacggctcgc catctggctt ctgcgtgcga tgccggagct gcgcgcggat
      781 gcgaaggatc agctcgcgca cgctgtacgg cttgacgacg tagtcatcgg cgccgacttc
      841 gaagccgacg acgcggtcga tctcctcgcc cttggccgtg cacatgatga ccgggatttc
      901 gcgcgtgcga tcctgctcgc gaatccgccg gcaaatctcc gtgcccgaga gatccggcaa
      961 catcaggtcg agcacgatca cgtccggcaa gggatccgtc gacgcggccg cgattccctg
     1021 gcggccgtta tgcgcgaccc ggacttcgaa gccctccgcg cgcaggttgt actcgagcgt
     1081 cgtggccaga tcgacctcgt cttcgatgac caggaccaga ccactcatga gcccttcgta
     1141 gcatggtgat ggtgacagca gtgtgacgac accgtggctg gcgtgtatcg ttcgccagcc
     1201 atgacgagcc ttgccgaagt catccccgaa cctctcaggt ccatggggcc gggggggctc
     1261 gaggtctggc aatggcttgg cctcggcgtc gtgctcgtcg tcagtgtcct gctcggccgg
     1321 ataggcgccg cggtcctgac gtggctcggc ggtaagctcg caggtaaaac gacgacgcgc
     1381 ctcgacgatg atctcgtcgt gaagctccgc tcgccgctgc gcttcctcgt cggtacaggc
     1441 gtcgcacgca tcggcatgct gccgctcgag ctgccgccgc acccaacgca gatcgtcatc
     1501 gaggtcctgc aagcggcgtt cgcgttcggc ctcgtgtggg gcgcgctccg cgcgatcgac
     1561 gtgatggcgg gccacctcgc gacggcgcaa tgggccgcga cgcgtccgtc atctcgctcg
     1621 ctgctgtctc tcatctcgcg cgtcgcgaaa gtcctcgtga tcgtcatcgc gggcatcggc
     1681 ttcttgagcg gcatcggact gcccgtcgcg agcctgctcg caggactcgg catcggcggc
     1741 atcgcgctcg cgttcggcgc gcagaagacg gtcgagaacc tcttcggtgc cgtcgcgatc
     1801 ggtgtcgacc aaccatttcg cgagggagat ttcgtcaagg tcgaagccga cacgatgggc
     1861 acgatcgagg tcgtcggtct ccgctccacg cgcattcgca cgctcgagcg caccgtaatt
     1921 tccctgccga atggccggct cgccgatatg cgcatcgaga cgttcgcgca ccgcgatcgc
     1981 atccgattcg cgacggtgct gaacctcgtg tacggcacga gtgccggcgc gatgcgcgag
     2041 gtcctggccg agctcgagaa ggtgctgagc gaccacacgg gcaccgtcaa ggacgagatc
     2101 accgtgcgct tcctcggctt cggccagtcg tcgctcgatg tcgaggtcat ctgctacttc
     2161 atgaccgccg acatgccgca gttccgggcc tggcgccagg aagtgctgct cgcgttcatg
     2221 gatatcgtcg agcgcaagag tggattcgcg ttcccgacga gaaccgtcca catgctacaa
     2281 agccgcccct gatgctgact ggcaagcaac gacgccacct ccgcgctctc gcgcacgaca
     2341 tgaagccgat cgtccagatc ggcaagggcg gcatcgacgg cggcctcatc agcgcgctcg
     2401 accaagcgct ctccgatcac gagctcgtca aagtgaaggt cggcgagaac gcaggcgtcg
     2461 atcggcacga agccgccgag aacctcgcga cggcgacgaa gtctcaggtt gcccaagtcc
     2521 tcggcaacat gctgctgctg taccgccccg acccggacga tccgcagatc gagctgccgc
     2581 gcgcgaaggc tgcggcgggc gctgccgcga gcacggatgc ggacgacgcc gaagcggatg
     2641 ctgacgacga cgacgacgac gatgacgatg acgaatagcg cgactgcgct tgccgtcctc
     2701 atcgcggtgg cagcgtgcgg ctctgcggcg gcgccaaccg agtcgcgagc gccggtcgcc
     2761 gcatgcggcg cgccagcggg cggcaacgag cagatcacga aggccgtcct cgacatgtac
     2821 gcggcccttc gcacggacga tctcgcgggc tttcgcgccg tcaccgagcc gagcttctat
     2881 gcgttcgagg gtggcgagcg gatggacggc gatgcgctcg tggacgcgat caagaacgcg
     2941 cacgcgcagg ggaagcgctt cgagtggacg gtcacgcagc ctgtcgtcgt cgtggactgc
     3001 acaatcgcgc acgtgacgta cgtcaacgtc ggggcgatcg gcgatgcatc tagcatgcag
     3061 ccgaggtcgt gggtcgaatc ggcgctactg cggtatcgag gggaccggtg gcggatccag
     3121 ttctttcatt cgacgcgcgt ccctgcgaag tagcggcgta tgatcggcgc atgagccgaa
     3181 tcgggttggt tctcgcagcg ctctgcgtgg tgaccgcgac cgcgtgggcc ggcgatccga
     3241 atcgttggca agcgtgggtg tcgccgaccg acggcgagag taatgcggtc gatgtgtcga
     3301 tggtgagcgg cgatcgcgtc gcgtgcggca agaagccgtg gatccagctg cagccgcgct
     3361 ttcgcaatcg ctaccaagag cgcatcaccg ggcacctgaa gatcgagtac atcgacgtcg
     3421 acgggaacgg ccaggtagcg acgcccgagt tcgatcctaa cgcgggcgag acgaagatca
     3481 tggcgctcgc ggcgttctgt catgatccgc gtcagtcgct gaagttctcg atcgcggaca
     3541 tgaaatttcc ggcgcgcgag gcggagaagg cgcggctcga ggcgcaacgc cttgcggcgg
     3601 agaaagcggc cgcggaagaa gccgcgcgca agaaagccga gaaggacaag gcggcagcgg
     3661 agcaagctgc gatcgaagct gcgaagcgcg aagaggcgaa gaaagaagcc gagcggctgg
     3721 cgaaagaggg cgagtcgtcg cgcaagcaga cagcggcgga gattcgcgcg ggtcagctcg
     3781 agtccgagga atcccgcaat gcagcgcgcg aagccgaggc gtatcggcag aagcggcagc
     3841 tcgaggccga gaccgaggcc aatcgcaacg tgcgcgtcga cgagctcgcg gccgacacgt
     3901 tccgcaacgg cgagatcatc ttcggcgcgc caatcggatt tcagaatacg aagttcggcg
     3961 cggagacgga gggccgcttc accgtcggtg gtcgcgtgca gctgcgcctc tacaagtggc
     4021 tgaagcgtct ctcggcgcgt ggcgagccgc tgtctggctc ggggctcgag ctcacgctcg
     4081 aaggcggcta cgcgacgacg atcgacatcg gcgagaacag cagcggcgcg cattttcgag
     4141 tctccggcgg ccacgctcac gcgcgctact ggcacggctc gctcggcatc ggcgcgttcg
     4201 gcgagtggac gcactattgg tacgacccgc cgggcaatcc gaacggcacg tatgacctct
     4261 acgcgctcgg accggagctg gcgttcggta tcgtcgcgtc gcggtctctc gcgctcgagc
     4321 ttgcgctgcg tggcggcgcg gtggcgtcgg ggagcggcct ggcgttcgac acgtcgaccg
     4381 acttgttcgt cgctgcgtcg gcgctcgccg agctcaacta tctctacggc ggcgcgatcg
     4441 ctacgcgcta cgcgacgagc agcaccgtcg acagctggaa tgcactcgcg gtgctcggcg
     4501 tccggctgcc cttttagcgg cggcggcggc gcgagacaac gagcccgagc acgccaagcg
     4561 cgagcagcgc gcccgtttgg ccaccaccag cggcgcagcc accgacgatg tcaccgctgt
     4621 cgagagggtc gcccgtgcct tcgccggtgc cgcccgaacc cggatccgac ggcggcgcag
     4681 cgcccttctt cacggtgtac gtgcgcgtct ccgacttgac gttgatgccg tcgctgatct
     4741 cgatgacgac ggtgtgaacg ccttcagcga gcgtcatcgg cgtggtgaac acgaacgggc
     4801 ctgcgccggt cttgctgtcg gtctgcatgc cgtcgatctt gatggccgcg cccttgacgg
     4861 cgatgttgtc cgagccctgt gcatgaactt cgaagccggg cggcacggtg gcgttgtttg
     4921 cgggcgacgt ccacgacatc tgcggcgcga tcgcatcgcc gcccttcgtg ccgaggcgct
     4981 ccttgagcag cgagaccgag ttctggttcg tgcggcagac cgaaccgttg atgccgcact
     5041 ggcgcgccga gtactcgccg cactgcgacg tctgatcttg gaatgcgcgg ttgcccgtgt
     5101 agtcgaggta cgtcatcggg tccgacgcga gcagctgatg gtcgaggccg tagctgtgcg
     5161 cgacttcctg cgccatcact tcgcacgctt cacgcggcgt catctggaac gcgccggtga
     5221 acgtgaagac gatcgagttt tcgatcacga cgcaatcgag cgtgaacggc gacacgcctg
     5281 cggtgttcgc gtccatgccg acctgcgttg gcgagccacc gaacacggcc tccatgtgcg
     5341 ggacgttgcc cggatcgaca tcggtgatcg tgacgtcgaa gcgcgagaac agatccttca
     5401 tgcagctgac ggtctgctgc cagttcgtcg ccgtgacgtt ccacgcggga agcgagaccg
     5461 cctgcgtgac gatcgtcgac ttgttcgtgc gcgagtcgtt gtcgcccggt tggagcgtga
     5521 cgccgttctt gttgaggtag acgatcttgc tctgggcgag cgcggtgcta gtcgtgccgc
     5581 tcgtggtcgg gggcgacgga atgacttggc gcgcgaggat gcggccgctc ggattgacct
     5641 cgcccacgat gctgtacatc tggaggtccc gcgcgtacgg gtcgagcgtc atcggacggg
     5701 gagcctctgc cgaagcgatt ccggttccca tcaacatcag ggcgccggtc acgagtccgc
     5761 gaagatgcat gaccccgatg aagtgctcgg aacatgccag ccggggacaa ccgaccctag
     5821 cgagtcaact gcgcgtcctg ccggcgcttt tccatgacgc gatgcgcgag cagcaggcgc
     5881 tcgaatgggg gtgttagcgc tggattgggg gcagggtccc cggctactcg agtggtcggc
     5941 gtgtcaccgg cacgcggctt cgatgtcctt gatcgtcttg ggacaaccgg cggtcaggac
     6001 ctcgttgccg gtcgcggtca cgaccacgtc gtcttcgatg cgaacgccga tgccgcgcag
     6061 ctcggccggc acgtccggag catcggccgc gatgtagagg ccgggctcga ccgtgatcac
     6121 catgccgggc tcgagcggac gcgccttgcc cttgtgcgtg tacgagccga cgtcgtgcac
     6181 gtccatgccg agccagtgac tcgtgccgtg catgtagaac ttccgatacg tctgatcttt
     6241 gatcagatca tcgacattac cggtgagcag gccgagcgcg accatgcctt cggtcaagcg
     6301 gcgcacgcac agctcgtgga tttgatcgag cgtcgcgccg ggcttcacca tctcgaccgc
     6361 ttcgacctgg cagtcgagga cgatctgata cacgcggcgc tgcacctcgg tgaacgtgcc
     6421 gttcgcgggc cacgtccgcg tgatgtcggc cgtgtagccg ttgtactcgc agcctgcatc
     6481 gacgagcacg agatcgccgt cggcgactgc gcagcgattc tcgatgtaat gcagaatcgt
     6541 cgcgttctcg cccgcgccga cgatcgtcgc gtagccgggc ccggtcccgc cttgcttgcg
     6601 gaacgtgtaa tcgacgagcg cttcgatctc gtgctcgaac gcgccgggct ttccagcctt
     6661 catcgcggcg atgtgtgcgt ccgtcgagat ctgcgaagcc ttgcgcagcg cgacgagctc
     6721 ttcgggtcgc ttgtgcagcc ggagctcgtg cagcgcgacg cgtggatcga tgatcgcttt
     6781 cggcgggcgc ttgccgcgct tctccgtctt gcgcagcttc gcgacggcgt gcgtgatcat
     6841 caagtccatc tcgtcgtcga ggccgaccga gaagtgcagc tcttcgcagt tcgagatcag
     6901 ctcgccgatc ttcgacggaa gctcgctcga cggatacgcc gcgtcggcgc cgtactgctg
     6961 cttcgcaccg tcgatgcccg cgcgccggcc gtcccacact tccatctcgg gatcgcgcgg
     7021 ccgcacgaac atcacgacgc gctctttctc cgcgccgggc ctgagcacga gcgtcgtgtc
     7081 gggctccgcg aatcccgtca ggtacagcag gtcggaaatc tgtcggaacg gaaagaacga
     7141 atcaccattg cgcagccgct cgggcagcga gcgaacgatc gcgaccgcgt cgggaccgat
     7201 cgcttgcata taagcgtcgc ggcgagcggc aaacaccgag ggatcgaaca tcccacgtgt
     7261 atagcagggc ttttagctgc gcatcggaac gagcgacgtc gccgcaactt cagccgcttg
     7321 aaacagcagc tcgacgtgcg gctcagcagc ctgcgcacac acgacgaccg tctcgtcgtt
     7381 cgccagcggc acggcgtaaa agatttgccg ccactgcctg ccgtcgtgct cgtacgcgca
     7441 ctcggtaaac ggaatctcat cgggcgtgat gccctggctc acgagccgct gatacggcgc
     7501 cggcttgatg accacttcct ccgccgacac gggccgtgga cggaacgaga tgaactcatc
     7561 aatgccgccc ggtgcatgca gctcgaagta cggatcctca tcgcccggct gcgccggcac
     7621 gagcacgaca tcgaccggca gctcgaccat cacgcccgcc gcgatcgccg accactcgat
     7681 gcccggcagc ccggtatcga tcttcagaaa cacggcctcg agctcgacgc cgtcctcgat
     7741 ctcgagcatc ggcccgccat cgccaaagcg cgcgatgccc gacgcaaacc acacggcatc
     7801 accggtcgcc ggaacgtcgc ccggagtaat gcgcgcaact tcacgctcgc ccatcagcag
     7861 cacgagctcg cgatccgccg tcgccatcac gcggactgcc ggcgaaggcg ccaggagcaa
     7921 tccgtgtcca ccaatcccat acgttgcctg catatggcgc ggaccttagt gatgggccct
     7981 cacgaaggca acgaaaatct cgcgccgccg cagcccaccg tcggacagcc tgcgatcact
     8041 cgggcttctt cacgggcact tcgccggtat tcgacttcgt gtactcgagc gccgctgcct
     8101 tgtagtcggg gtcgtgcttg cacttcgtct gctggatcac cgcgccgact tcattcagca
     8161 cctgctgcag acgcgggatg tagtacgtct tcgcccacgt cacgagctcg acatttcctc
     8221 gcgacactgc atcgagcttg tcgctcgcat tgccgagccg aacgctgagc gggatgaact
     8281 ccttcattgc agccgcggca accgcacagt cctcggacag caggtcggcg cgcttcacct
     8341 cgagcttcgc cgacagctcc tcgacgacgg caaccgcctc gtcgtcgatc gcggccggct
     8401 tgcccaccgc ccactctggc agcggccgtt tttcggcctc cgctccagcg cttccagcgg
     8461 catcgattgc aacgctggag cccgcgttcg tcgcgaccgc tgcagatcca gagcctgctg
     8521 acgaaccgga gctcgctgca gaccctgtcg ccggcgcatc ggtcttcttc gaacaaccaa
     8581 caacgaacat gatcgcgaca accaacaacc gcatcgcgtc atcatgcccg aagtagctac
     8641 gcacggcggc gccggcggac gacgaaggcc acagcgagca cgatgaggac gacgccgcgc
     8701 gagttgccgc ccgcggacat gcatgccccg ttgtacgtgt catcgccgta gtaaccgtcg
     8761 ccgtagtcat cgccataggg atcgctggcg cacgtgcccg aggccgtgtc gccggtgccg
     8821 ccgtacgtac ggagattggt gagatagaga tcgatcagct ggatgtcagc cgcgatgtcc
     8881 ggatcttcgt gcgtgctgtt ccacgtctgc gcgctggtct tgaccgcgtt gagcgtcgag
     8941 gccgcgcagt tgtagtcgta cgcgttcgtc gccatgcgca ggccgaggaa gatgttgtac
     9001 atcgcgtagc gctcggccat ttcgggggtc gagaggtacg tgtccgccgg gatttcggag
     9061 ggctcgttcg ggtacgcgag cgtgatcgtt tgtgtctgga tttcggacga gccggggagg
     9121 cgatacgtga gcgtgaggtc ggcgactttg ccgtcggggc cattttggcc ggtcggcgtc
     9181 aggtggatga acagcatgct gccaccaccg cggcgaccgc cttcggggcc ttgcgacgtg
     9241 cggctcgcga cgaagaccgc ggggatcgcc atcgagccgg cagtcgtctg cgatttccag
     9301 agcgtggagc cgatgacttc gccgaactgc cagcccgagc ccgatgtcgc gttgacgtgg
     9361 acgtcgagtg cgagcggcgt gacgaagtag tcgagctcct ggttgaagac ttcggaggcg
     9421 gccgctgcgt cttcgaggaa gtagaagttg ccggcgccgt gctcggcgag gccgcgcatc
     9481 agcgagacgt cgaacgagag gccgacgccg atcgtggtga ggccgatgcc gtcggtgacg
     9541 tagcggtcgg ccatcgcgat gatgtccgtc tggctcgtgt tgcccgcggt cgccatgccg
     9601 tcggagagga agacgacgcg gttctggcgg tcggaggaga aggccgtggc ggcgagcttg
     9661 aagccttgct cgaggccgtc gtagaggttc gtcgagccgc gcggcgtgag gttgttgatg
     9721 acttgatcga gctgcgtctt gttgacggtc tcggcgaacg gcacgagcac gtcgacgcga
     9781 tcgtcgaatt ggacgagtgc gaggcgatcg ccttcgccga ggttttgcac gagcgtgtgc
     9841 aggccgctct tcaccttctc gaggcgttgg tcgtcccaca tcgagccgga gtgatcgacg
     9901 accacgacga gattcagcgg cttgcgcgtg atcgttgtcg gatcgacgtt cgtgttgact
     9961 gcgatctgaa gcgtcgcttg gtgtgcgccg gtgagccagt cgcgaccaac ggagacaccg
    10021 ggcgtgatgc agagcgtcgt cgtgcagtcc gtcggcggcg cgacgtagtg ctcgttgaag
    10081 aagccgttcg cgtcgagcgt ctgggggccc ggaatctggc cggcttcgag gatcccgcgg
    10141 aactcgccga tgtcttgctg gccgccgaag ctgacgttgc cgccgccggg gctggcgctg
    10201 tcggcctggc cggcgcaacc ggcaatcgaa acgaggagaa gtaagcgctt catgccccct
    10261 cctacagcag cgcccgtgcc cgcaatccgc gagggtctgc ggtcacttat ctgccgcgag
    10321 ttgccgccga tcgaggaagg cctgacatcg atgtcgtagg atgcgccgca tgcgccgcct
    10381 gttggttttg ggcctcagcc ttgtcttcgc cgcctgtagc tcgcagccgc cgaagatggt
    10441 cgtaccagcg ccggcgccgc cgccgcctgc cgagatgccg gcgccgccac gcggcgtaac
    10501 ggatgctgcc gtggcgatcg caccggatgc gggcccacct ccgctgccgt ttcgcactgg
    10561 cgcgtcgggc ttcaacgtcg atccgaacgt cgccggtacc ggcaagacat atatggtggc
    10621 gacggaagac gagtacgcga cgaaggcggg cgtcgcggtg ctcgcatcgg gaggcaacgc
    10681 ggtcgacgcg gcggtcgcga gtgcgttcgt gctcgcggtg acgctgccga gcgctggcaa
    10741 catcggcggt ggcggcttcg cggtcgtgcg ctccggcaag aacaagatcg ctgcgctcga
    10801 ctttcgcgag accgcgccgg ccgccgcttc gcccgacatg tacaaagacg atccgaagag
    10861 ctcgctcgcc ggctacaggg cgtccggtgt gcccggctcc gttgcgggtc tcgaagcgct
    10921 gcacaagaaa tacggcaaga agaagtggaa ggagctgctc gcgcccgcga tcaagctcgc
    10981 gcgcgatggc tacgtcatca acgcgcacgt gaacctcagc atcgtgctcg gaaatcgcgt
    11041 cgcgccgatg agcggcaatc tcgcggcgat ctatctgccg aacggcaaag cggtcgagac
    11101 aggcgcgacc gtgaagaacc cggagctcgc cgacgcgctt cagctcatcg cggataaagg
    11161 cagcgctggc ttttacaaag gcacaacggc gaaggcgatc gcaacggcga tgaaagccaa
    11221 cggcgggctc atcaccgaga aagacctcgc tggctacaag gtcgtgtggc gcgagcccct
    11281 gaagtacacg tatcgcggca agacgctgta cacgatgccg ttgccatcgt cgggaggcat
    11341 ggtgctcgcg ctcaccgcga acatgctgcg cggcatcgat ctcgcgaagc tcggctggca
    11401 ctcgacgcaa cacgtccact ggctcaccga ggtctggcgc cgcggctatg cagcgcgcaa
    11461 cctcgcgctc ggcgatccgg cgtacgtgaa ggacatgccg atcgcgcgac tcacgtcgca
    11521 agccgaagcg gatcgcctcg tcgcgacgat caccgagcgt gcaacgccgt cgaaggatgt
    11581 gccgccgctg atcgaaggca agcacacgac gcacttcaac gtcgtcgacg cgaaggggat
    11641 ggcggttgcg ttgacgacga cgctcaacac cggcttcggc agcggcgtga tgatcaacgg
    11701 gatcatcatg aacaacgaga tggacgactt cgcgacgatt ccggggcagc cgaacgcgta
    11761 cggcctcgtg cagggtgaag cgaacaagat cgagccgggc aaacgaatgc tgtcgtcgat
    11821 gtcgccgacg atcatcgagg acgacaaggg cgagctctat atggtcgtcg gcgccgaagg
    11881 tggctcgcgc atcatcaccg cggtgtggca gacgctctcg aacgtgatcg actacggcat
    11941 gagcatcgac caggcgatcg cggcgccgcg cattcaccac cagcatctcc ctgatgacgt
    12001 cgtgttcgac gacgaagcga tcaccgcaga cgtcgacgcg gagctcgaca agctcggcta
    12061 cgtgcgcgtg tggaaccagc gctcgcgcat tttctcgtcg gcgactggga tcgtgaaaac
    12121 acccgctggg tgggcgggtg ctgcggatcc gcgccgaggc ggcgctgcac taggcgacta
    12181 acgccgacta acgaacgagc gcgtcggccc actgcgcgac gtggttcgac gtctcgagcg
    12241 actggaacgg gacgtggaac gcgggacgcg cgaagcccgt ctgcgggaag aacgccatca
    12301 tccagatctg gcggccgatg tcgttcgcgc cgtactcgcg ctgcgaatcg aacgtcaggt
    12361 agtagaacgc ctcgccgttg aggctgtgcg cacccggtgc ccagcgcgct gccatgtcga
    12421 cgtccgaggt cggcgcgacg agctggatcg gttgctcggt gccatccgtc ttgatcaccc
    12481 agatcgacgt gagcggcgag ctcgtgcccc aaccgctcgt gcgcgtatac gcgagccact
    12541 ttccgtccgg cgaccacgac ggcgagtagt tcgcgacgcc cggcccgcca ccgacgagct
    12601 gcgccggatt cccaaacacg ttgccgtcat ccatgaacgt gcgcacgacg aggttgccgg
    12661 taccgcttga cgagaagccc gacgactcga cgctgacgag cttgctgcca tcgggcgaga
    12721 actgcggatc catcgcggtc ttgttcggcg tgttgacgag cgtggcgagc tcgccgccgg
    12781 tctcgtcgat gagcttcagg atgccgtctt gcgcgatgac gagcttgtcg gcgttgcgcg
    12841 tgaacgtggc cgagctccag ctggtcggtt gcatcacgcg gtcctgcgtg aagtcgagga
    12901 tcgtgccgat gccgctgtcg aggcgaagcg ccatgcgcgc gccgctgcgc gagaccgcgc
    12961 aaccgctgca cgacgtgacg agcggcgccg tttgcatctg cgtgatgttg ccggtgcccg
    13021 gcatgatcat cggctcgggc tcgccgcgcg ttgccgcgtc ctggcgccag atgccgccgc
    13081 catcggacca cgtgtagatc gcgccggtca gtggcttgtc gctgacgagc aggcgacggt
    13141 cttgcgacgt gcacaccttc gtcggcgatc cgctcgagag gctcgacacg tgaaacatga
    13201 tcggctcgtg cgtgctggcg agctgcgccc acgcgtccgc cgagagagcg cgctcgtggt
    13261 tgcgcgtgta gagctcgacc gtggcgtatt gggtcgcgaa cgagacctcg aacctgtcgt
    13321 tcgcgaagtc cgcccactgc gcatcgatcc catcgaggtt gctcggcagg atcgtcccac
    13381 cctcggggta catgacgaac ggaaagcacg tctcgtccga gcgcagggca tcgaaccacg
    13441 ccggcatgtt cacgacgtct tcttcgacga tgcgcgtgtg tgcgtacacc gtcaggttcg
    13501 tcgatgcggt catgccttcg aacgtcgccg tgatcgcgga cgggccaacc gccgcgccgc
    13561 gaagctcgag cgagctcgac tgccacgtgc cgaggtccgg ccgcgcgagc gcgaacgtcg
    13621 actccgccgt gacgtctcgc tcggagccgt ccgcggtgac gagcaatgcc cggtactgcg
    13681 tcgagaccgg ttgtccatcc gtcacgacct ggctcacgtc tgccggctcg acgcgcaacg
    13741 tcgcagtcga atcgtcaacc gcgtccacac cgcaaccgac gagcgcgaga acagcaacgg
    13801 ccgtgtggtt cgtgagcctc atgacttgtt tcatcgaccg cgagcaaaac tctgttatgt
    13861 gcacgcgagt gcgacatcgg cctcgtggca tgctggacgg cgatggcgga gaagaacgat
    13921 ccgagtggga tcgccgttta tccgaagcta ccggttccca agagccggac tagcgagccc
    13981 aaggtgaagg gcttcaagga caacgacggc ttcagcattc ccaaggtagt cgcggcgttg
    14041 gtgatcgcgg cggcagttgg cggcggtgta ggttttctgc tcgcgccgtc gaaggccgca
    14101 gatctgaaga aggcgaagac cgacctcgcc gctgcgcaga ccgcgatgaa agtcgagaag
    14161 gaccgcgccg acggagccga acagcgcgct gtcgtcctcg agaaggacaa gacggagctc
    14221 gcgcagaagg tcaccgagct ctcggcaaag gccggcgaga tggaaaagaa ggtcgccgac
    14281 gccaacgcgg ccgcggagaa gaagctgtcc gccgcgatcg ataaatcgca aggcacggtc
    14341 tcgacggaag gcgacgagat ccacctcaag ctcgtcgaca aagttctgtt cgcggtcggc
    14401 gacgatcagc tcaccgacaa gggcaaggcg gcgctcgaca aggtcgcgaa ggcgctcgcc
    14461 gagctgcccg acaaacaagt gtgggtgcag ggccacaccg acgacacgcc gattgtcatc
    14521 ccgcctcctc cgaagcccga cccgaagaag aagaagggtg caaagcccga agcaccggtg
    14581 gcgccgatgg ttcgcttcgc atcgaactgg gagctctcag ccgctcgcgc gcttcaagtc
    14641 gttcactacc tgcaggacca cggcaagatc gatccgacgc gcctcgccgc gctcgcgttc
    14701 tcggagtacc gacccgtctc gaagtcgaac aaggcgcaga atcgtcgcat cgagatcgtc
    14761 ctctatccgc acaaggccgt gatcgagaag gcgacgaaga agtagtcagc gcgccgtcgt
    14821 gcacttgtcg acgaacgaca acaggtcgct gtcggccgca tgcgcgagct cgacgccgcc
    14881 cgcgtcgtcc cgcgagaaca gcaggagcgg cagcggctcg ctcgagtcgc gaatctcttt
    14941 ggcgacgtct gcgacgcctt cacttgcgag ctcgagcagg atggcccgcg ggcggagcgc
    15001 gcgagcgagg cgcgcgagct gagccggtgc gcgagtcatc gacacgacgg tgtagccgcg
    15061 ttccgagagc acgacgtcta ggcgttgcaa gcgcgcgcga tcgtgctcga cgagcaggat
    15121 cacacctcga tcggaaggct cgggcgtcgg ctcggagggc gcagcgatga atggtgagtg
    15181 agcgacgcga tcggcatctc gcgagcgata gacggcgagc gcgaccttgt tcagcaggtc
    15241 atcggcgtcg aatggttttt cgacgacctc gctcgcgccg atcgaagcga gattcacctc
    15301 gaggccgtgg accgcggtca tgatcaagac cgggacttgc tcgtagctcg ggtctttgcg
    15361 aagagcttgg aggaactgcc ggccgttcat gccgggcatc atcaaatcga ggaggatcgc
    15421 atcggggcgc gccgctttga cgcgggcaag cgcgtcttct ccgtcaaccg cggtgtcgac
    15481 gacgtagcct tcatcgacga ggacttggcc gagtgtgtcg cgcaccatcg gctcgtcatc
    15541 gacgatgaga attcggcccc ctctcgcaac ggctcccgac atgacgactc tcgtccacag
    15601 cgtacacccg cgcggcacgc cctggtacca tcgccttgat gtcgacggcg cctccggtgg
    15661 cacgtgccta cgacttgttg cgcccgaaga tcttgcagcg tttgctggag cttcggacga
    15721 cactcgaccc atcgcttggc cggctcgacg agagcacggc tcgcgcacaa atcggtgcgg
    15781 tgctcgatca cctcgggaac ttcatcgcga cgggcgatct cgggttgcac cgcgcgttct
    15841 tgcacacgtt cctcgcgatg cgcgcggccg aggcacaggg tcccgctgcg gtgctcgcga
    15901 tgctcgtcgc gatcggcgac accgctgcgc aggtctcgca ggaagaaaat ccgaatcctg
    15961 agggctcgga gctgacgctg ctgctcacgc gagtgaccgc gtcgacggca cgcgccatca
    16021 acgacttgat cgctgaagag ctcgagcggc ggatggcgca gtgggccgag ctctcgacga
    16081 aagagcgaga agggctcgag ccggcgtgac cagccaaccg cagcagcccg gtctgcgcat
    16141 cgcaggcgac acgcacgtcg gctcggtccg gacgacaaac gaagactcga tgatcgtcga
    16201 tcatcagcgc ggtctctatg tcgtgctcga cggtatgggc ggcgcgaatg cgggcgacat
    16261 cgcttcgcag actgcgcgcg acgcgatcgc gacgttcgtg caacagcgcc ggctcaacat
    16321 ggagcccaaa cagctcctcg agtccgcgat ccagtacggc tgcgccaccg tgttcaatgc
    16381 ggcgcaacag gcgcgcgagc gccacggcat gggcacgacg tgcgtcgcgt gtctcgtcat
    16441 cgataccaag cgcgcgattg ttgcccacgt cggcgacagc cgagcctatc tgcttcggta
    16501 tggacgtctg caggccctca cccgcgatca cacgatcgtc gaggagctcg tcgagcgcgg
    16561 tgtgctcgca gccgacgaag cagagcgcca tccctacaag aacgtgctgt cgcgaaacct
    16621 cggcgctcgg cccgagaccc gcgtcgacgt gctggagctc gagatcaagc cgggcgaccg
    16681 gatcatgctc tgctccgacg gactctacgg ttacgcatcg aacgatggca tgcagtacct
    16741 gctcggctcg ggagatgccc ccgagaccgt cgcgcacgat ttgatcgagc tcgccttgcg
    16801 cggcggtggt ggtgacaacg tctcggtgat cgtgatcgaa gcaccgcagc ccgcgccgag
    16861 ctcgacgcaa gtcgtgcgga catcgggcgc gaccgcgtgg tggcaacatc gccagcgctt
    16921 cctccagatc gcgaaggatc gcggcatcac gaagaatccg atctgtcgcg gactcaagcc
    16981 cgaagaggcg ctcgacctcg tcgcggcgtc gctgtgcgaa gcgatctttc acgatctcga
    17041 gaagtcgacg ggcgtcaacg tgtggacgtt cgcgcagaac gtcgccggcg gctggttcga
    17101 gcgcggcggc gaatggaaac cgctgcgcga tctcatggac atcctcgcga tcagtgcacg
    17161 caccgtcgtc gaagatgttc gcaaggcgga cgcaaagctc ggcttcctcc tcgatgtagc
    17221 ggtgtctcgc gcgctcgtcg tcgcggagct ggcgctcggt ggcctcctcg cggagcgctt
    17281 gcgccacgtc gatcaggatc tgatccagat ccacggcgcg atgcaggaca cgatcgatgg
    17341 cgatgtcgag cgcgccgata ccgactcgcg tttcaccgat caggcgacga ttccgttctt
    17401 gcggcccgat cgcccggtca cgaatcccgg catgagtgca gagctcgtga ccgcgatcga
    17461 gaagacgctg acgatcgcgc gtgctcgcac gtcgccgcgc gcggagctcg tgaagcagat
    17521 cctcatcgcg ctcgagacga tctcgacgga tccatctggc aacttcgcga gcgcggtcct
    17581 tgccgcgcgc gagctgtacg gcgttcgcag cgtcgacgat tcgggcatca tgccgctgtt
    17641 cgacgcactc gatgcagcgc gcatcattac cgcctcgagc gtgcaccaga tctccgcgcc
    17701 cgatgcgatg cgagcgaagg tgctgcgtgc gatcagctcg gcgcatcaac gcctcgtcgg
    17761 tgcgatgacc ggcctcgtgc tcgaagccgt cgcgccatac tcgcaacgcc tcgaagaaac
    17821 gaaggccgtc accggcgagc tgcgtgacaa ggtcgcgagg gccgagcgca agcgcgccga
    17881 gctcgaacgc aagttcgcgt cgacgatcga ccaaagcctg ccgtgggaac gccgcggcac
    17941 gacggagtgg taatggctga gcccgaagtc gatctcatcg aagccgtgaa ggtcctccgc
    18001 gacgaggtgc tcgagaccgt cgagcatggc gatcgtgatc cgcccggcgc ggaagtgttc
    18061 gacgcgctga tcaacgcgct caccgtcggt ggcgagtcgg ttccggggct cgacctgcag
    18121 ctccacgatt cggtcacgcg gcgtctcgcg tggggcgaca gcgaagaagt cgtgctcgcc
    18181 gatgcagagc gcgtcttcga tcgcctgctg ttcgccgccg agcgcgcgtt ccgcgagcct
    18241 gccgatcgca tggtcgtcat cgaagcagcg acgcaggtcg ccgtcaccgt cgcgcgtgtc
    18301 gtctcgctcg ccgcggtcgg acgcgcatca cgcgatcgcg ccgcccgttt gcgcgaggag
    18361 atggcccagc gtcagctcaa agacgtcctc gagaagcagc gctcgagcat cgtgcgcctc
    18421 gaattcgaga cgacaaccga tcgagcgccc agcgaaaagt aactagggcg ctgcgatgtt
    18481 cgcggtcacg gcatcgagca atgcgtcgag acccgagtcg cgatcgacat agcgatggac
    18541 gaggtcatcg tcgctgcgtc cgaggccgcg aacgatccgc cgtgccgcgg gttgctgctt
    18601 gcgcacgtca tccatgatcg gctggatgcg cggatctgca gcgtcgagct cggcgatcac
    18661 ggcatcgagc ttgatgttct cgagtgcctc gtgcacggcg aagtcatcgc tcgcgaacag
    18721 cacgtcccaa ccactcgaca cgtagagcgc catcgcgccg cgctgccgat gatcgtcgcc
    18781 gaccacgagg acgcgcatgc ggcgaccggg cttctcgacg gtgcccgact gccgcgcggc
    18841 attcgtcacg agcaccttcc actcgggcgg tggttcgatg agcagcacgc cgagtgccgg
    18901 gttcgaaccg gcgacttcac gcacgacatc gccgatcact tccatcgcgg tgccggagcg
    18961 cggatccgtg agcgcgagcg agacgcgcgt accgacgggc agccactccg aaacctcgga
    19021 tgtgtcgagg caaatcccga catcagagac gtcgcgcgta tggcacacgg ccatcttgcc
    19081 gccgagcaac ggcatgaccg tcacgttcag cgaaatccga taccggggtg cgcgccgaga
    19141 caccgggaca cattgtaact cagcgcgaga ctccagggcg gcggggtgac aggcagcgac
    19201 aggctcggcc cgattgaccg aggggggatg ccgagggact gtacgaggca tgagagccct
    19261 cttggcgtgc gccgcgatgc tcgtcgctac gcgcgcgtat gccgacgaac cgcagccgac
    19321 accgacgggg ccgacgctgc caacgtcgcc ggcacccgca cccgcgcctg gaccgggtgc
    19381 cgagaccgcg ccgtcccaac ctgcgacccc ggaaccgaag gcggatcccg cctacggcga
    19441 gaaacccgac cctgcgtacg ggccgccccc gggcagcacg gtgcccgaat cgtcggggcc
    19501 ctcggcgggg agggacatct acatcaagtc gtacccggac cgttcgcaca agaacgtgat
    19561 gggcctctcg atcgcgggcg gcgcggccgt cgtgctcggc ggagttggtc tgtattttca
    19621 tctcgattcg cgcagcaagt cgaacgacgt cagcgcgaac aagtacacgg gtgaagtctg
    19681 gacggcggag agacaagaca cgtacgactc ggcaaatcgc tctgcagtcg tcgccggcgt
    19741 cttctacggc atcggcggtg cggtcctcat cggcaccgcg atcgcgtaca tgatcaccga
    19801 gccgaagatg gaaacgaccg ttcttcatcc gcacacgagc cccaagccga ccgcgatggt
    19861 cgcgcccacg cgcggtggcg cactcgtcgg cggagcatgg agcttctgat gcgcgctgct
    19921 gtactcctca tcgcgctcgc ggcgtgcacg ccggatgtct tctccgaatc ctatctctgc
    19981 ggacccgacg cgttgtgtcc cgagggccaa gcctgcaacg gccccgacaa catctgcgtg
    20041 ctcaagggag cggcacagcc gttctcgtgc acgccgaaga tcgcgaccga gcccgacgac
    20101 agcgcggcga acgcacacga gatcgcaaac ctcctgtgcc cgtcgctgcc gttctccgac
    20161 gagaactgca tgctgcaaga cgacgccgaa gattgggtga agctgaaggc gccggcgatg
    20221 tgcaacacga acgcggtcag caccgtgaag ctcgcggcgg gtgtctcgtt cccgatcgcg
    20281 ttcgagcgca tcgcgatcga gctctgggat ctgggcacga tgacgaagct cgccgatgac
    20341 gcggaatgca cgaggggcga aggcgtcgtg ggcgaagaga ttcgttgcct cgactacgcg
    20401 ctcgtggcgg gttcgagcta cggcatcaag gtgcgcccgg ccggcgcggg tgacggcgac
    20461 tgcgacggaa actgccgctt caaccgctat acgttgcgag tcgagctctc cacgccgtag
    20521 tcttccaagg tgaccgagct gcggtggagc tggcaggcaa cgccgtacct cgtctgtacg
    20581 ctattgctcg tagcggtcac cctcggcgcc gcactcattc gtggcgaccg cgtcctgcgc
    20641 atcggcgtga ttgcagcggc catgaccgcg atcccgtggg cgctgtgcca agcgctcgcg
    20701 gcttgcaccg acgacgcgat cgtcgctgct cgctttctgc ggctcggcca ggggcccgtc
    20761 gcgagcgtcg gaccgaactt gatgctcgtc attttgggcg tgagcggtca gctcgaacgc
    20821 tatcggtggg tcgcgcgatt ggccggtctc gtcgcgacgg ttgcgctcgt aatttgctgg
    20881 gccaccgact gggtcgtccc cggcgcgcag cgcgtgcctc tcggcatgta ctacatgaag
    20941 cccggcccgc tgaccggact gcacatctcg cagctgctca tctggctcgg cgtcgggctc
    21001 ttcatcgcga gacgcgccgc gccgggcggc agcgagcgca aacggctact gcgtctcttg
    21061 ctcggcgtgc tcgtcgccgg cgccatcggt tcgatcgaca cgctgctgct ctatcagatc
    21121 gccgggatct atccgatcgc gtggctcccc gcgtcggtcg cgtgcggcat cgcgctgtac
    21181 ctcatcttca agacggatct gttacggccg caggggttcg atcgaggcat ggcgatcgag
    21241 atcgcgatgt tcggcgcgac gacgatcgcg atcgccgcgc tcgcgcttgc actcggcaca
    21301 tcgcacaaga ttccgctcgc cgcgttctcc gcgttggcgt gggcgatcgc gaccggaatt
    21361 gcgtggtcgc tcattcgcgc gcgcccggtt cgcgtcaaag gtgagcgcga gctcgagcaa
    21421 ttcatcgggc gtgtcacgac gtatgacgat cagaagaaga tcgccgaacg cctcggcgct
    21481 ctctggaagc gcgcggtcgg catcgacgtg aaggcgatgt ggtgggtcga gggcgcggcg
    21541 ctgacaaaca gcagcggcga gaagtggacc atcgatcgcg aggtgagcga ctggctcgtg
    21601 cagcacgacg acccgctcgc gttgacagat ctggcaacga tgcgacttgg tgacctccgc
    21661 gcgaaggtcg aagctctcgg cgcagcacac ggcgcgacga tgctcgttcc gctcgtcgat
    21721 cgtggcgagc tcgtcggtct catcgaagcg gattactcca aggcgctgcg tgatgccgag
    21781 cgtgggctca tcgacgagtc ggcgcgagcc gccgcgcgct cgctcacgtt cgtcggcctg
    21841 gcgcgtgctg cggcgcgaga gcgcgagacg gcgcgcgagg tcgaagttgc cgacgcgttg
    21901 cggttgcagg catccgcgag ccgcgatgca gagctcggtc gatgggcggt cgccgccgaa
    21961 tatcgaaccg ctgcacgcac gaccggcgcg ggctggtccg cgatcgagct tccagatggc
    22021 cggctcgcat tgctcgtcac cgaagcgcaa gcacaaggcg tcgcggcggc actagcgacg
    22081 gctgcactca ccggtgcatt cgcggccgcg acctccggca gcggtacggt cacgcgcgac
    22141 ggtctcatct cgacgatgcg tgcgagctcc gaaggtgtgc ttcgcagtgg ccaacccgtc
    22201 tcggcgtttc tcgcgatcct cgatgcgcaa gcgcagacga tcgagtgggc gtgcgcgggc
    22261 catcccggcg gatttctcgt cggaccgatc gccgcggtcg atgcaggtct cccggcaggc
    22321 tccacgaagg gcgcacgtcc gaaagcgacg ccgatcacgg cgggcaagcg agtgaaagac
    22381 gcgagcctca cggccgctat ccgcgggaac tctgcgttcc atcccgacac acttctcgtc
    22441 gttgcatcga cggggctgcg cggccccgac gacgaatcgt ggcagacgag gctccaagaa
    22501 ttcgcgccgg cgagtggacg cctcgcgtct gtgctagtcg acacggcgct caaggcaggt
    22561 gagccggcgg aggacatgct tgccgtcgtc gtgcgcgctc gctagccatg ttacgatttt
    22621 gaaatcatgc gcttacgtga cgtgctgatc gcattgactg tcgccggctg cggctcggtt
    22681 tctgataacg gcaacatgcc agacgctccg gcggacgccc ctgatactgc ggcgccgatg
    22741 atcatcgact cgcaccccgc gaaggacggc aagggcgcga gcgtcatcca gcccatcacg
    22801 ttcgtgctcg acgaggcggt ggatccggcg accgtaacga ccgccactat caaggttcgc
    22861 gcgtggatga aaggcgcgct gacagacctc cttttctcgg gtggcccggt cgagaacgcg
    22921 atggcgatcg aaggcactgc gacctacgag gccgcaacgc acaagatcat cttcacgccc
    22981 gttgtgccgc tgccgttcaa cgcccgtgtc gaagtcacga tcgccggcgt caaggacaca
    23041 gccggcaatg cgttggcgac gaacgtcgcg ttcgacacga tccggaacat ggaggccgct
    23101 cagatcttct atgcgaccgg caacgcgatc tcgcagtact acgacttcgc tgtcgactcg
    23161 atgggcatgc agaggaagtt ctcgcgagtc cccggcccgg gacccgacgg gagctttttc
    23221 accgccgacg acccagtcac gcagcacttc gacatggtct tcgacatgaa cggtcgattc
    23281 atcgtctcgc acagcttcca ggccggcacc gacggcatgt tttacacgtc ggacgatgtc
    23341 gagaccgcag cgctcacatc gacgtacgac atgagcggtc gcctcacgca cctcgtgtcc
    23401 aacaacgcgg cgggtccgga cggaatgtgg ggcaccgcag acgacgttcc aaactcgatg
    23461 tacgtgcaga gctggatggg cgacaactcg ctcggcgtga tcttcttctc gtcgcccggc
    23521 ctcgacggcg tgtggcgcac cgccgacgat cagatgggca gcccgacgac gcagttcacg
    23581 aagtacgagt atgactcgat gggccgccgc acgcgccagg tcgtgaatgt gcccggcgca
    23641 gaccgcgtgc ccggcacagc cgacgacacg aagcagcgct atttcgatct gacaaacgac
    23701 agccacgggt tcgtcacgcg cagcgtcgag cgcaccgcag gttcgaatgg aatcttcttc
    23761 gatgcggacg acacgaacat cgcgtacacg cagacgaccc gtgacgcgcg tggcctcgca
    23821 acggagatca agctctacga caccgcaggc gtcaacggca tctggttcga tgcggacgac
    23881 cacgtgtcgg tcggcagcgg gacgcgcaga acgttcacgt acaacatggc gggacaggtc
    23941 gtcgaagaag tcagttggct cgccgggtcc gatggcatca tggggaccgc ggatgacgtg
    24001 atctcgacct acctcaccac cgtgtacgac tcgaagggag ctcgcgtttc ctacacgtcc
    24061 accacggggc aggggccaga cgctctctgg cgcacctcag acgatcagtt gggcttccgc
    24121 cgcacgtacg acgcgctcca ctagcgtccg agctgcggca ggagctggtc ggcttgctgc
    24181 gaccgctcga cggcttgctg gctcgccgag aaattcaccg gccgcagcgc ggcgagcgcg
    24241 agcagcgcca ggacgaggac gacccacggc cagtgacgcg gtcgggcctg cgcgaaggtc
    24301 gtcggcagcg cgcgagctgg aggatcctcg gcgggcaggc ggggaacgag cagcgtgtcg
    24361 tcaaagctag tgtcgtgcat cgcgcgtcgc gcttgtgcga ccgcagtgcc agcgcatcgt
    24421 gacgtcctga tccacgccgc gacgcgaagg ggcacgcacg agtcgtgcaa cgcgtgacac
    24481 agctcgcctg cgcgtttgca cgacaagatc agcgcacgca caactatgca tggggattag
    24541 ttctccagcg tggtgcaacc tgttcgcatc gctggttgag cgactgcctg ttgccgcagc
    24601 gcggcgggca ggggaagctg gcctcgatga aacgcgccct cgcgctggtt gtactcgtcg
    24661 cgtgtggcga taactcggtc ccgccggccg ccgaggagat ggtcgtaccg ggctgcacgg
    24721 acatcccgca gccgggcccc gatccatttc ccacgtcgga gagcgtcgac gcggtcgcgc
    24781 agggacccgt cgttccgggc acgctcgcac actggaatct cgatgggcgg tggtttctca
    24841 cgggcgtgcg ggtcggcggc atctcgagct atcacttcga gcgctccggc gacgtcgtga
    24901 tcgtcgatcg cgacaatggc caccccgcca cgttcgatga cgacgtgttg ttccaacgca
    24961 gcgtcttgca ctcgaatggg atggatttcg tggtcatcaa gcgcgtctcc aatcgccacg
    25021 ccgatggatc gctgcgcgcc gaacgcatct tgtgcaatgg cgatggctgc cgcgtctgca
    25081 ccgcgaagat gatccgagcc gagcgcaacg ccggcgaatc cgagggcgac cacatctcgt
    25141 tcgtcggcga gctcgaggat ccagcgtggc ccgactcgat cgcgctgaat gtccgcgtcg
    25201 tcgacaacct tgcctatctc gtgcgtcgcg acggcgtgca catcatcgac gtcagcgatc
    25261 cagcacatcc gacgtcgatc ggcgcgtggg gtagaccgtc accgtcgggc tcgtcgaacg
    25321 acgtcaagat cgtgcaagcg gggccgaagc gctacgtgct caccggcgac gttcctgtcg
    25381 acatcatcga cgtcactgac cctgcacatc ctcagcttgc cggccaaatc gccgaggaag
    25441 cgcacacgct tgccgtcgag actcgagaca acaaggtgta cgcctacttc gggaactacg
    25501 acggcaagtg tccgatctac gacgtcaccg atccgacgca gccgcaaaag ctcggcagct
    25561 tcacgacgcg cggcgacctc gtgcacgacc tctcgatcga gaacggcatc gcgtatctga
    25621 acgcgtggaa tgacggcttc tacgtcgtcg actacacgac gccgagcgct ccgacgttag
    25681 tcgggcattg ggccgggacc ccagcaggat cgagtcactc gagttggccg atgaccgtcg
    25741 gcggtcggca catcgcactg cacggtgacg agaacttcaa cgcgcacctc gacgtcgtcg
    25801 atgtcgatcc gagctcgcca caattcatga cgtcgatcgg ctcgtatcag acgcgcgact
    25861 tcatctcgat tcacaacctg atggcgttcg gctcgaaggc gtatttcacg tattaccaag
    25921 atggcgtgcg cgtcctcgag atggccgatc cgacgcagcc gcggctcgtt ggctacttca
    25981 acacgtggga tccgaacggc gagttttcga gcaacggttt cttcgaaggt gcagtcggcc
    26041 tcgacgtcga tgttgcgcgc aagctgatct tcgtcgcgga catcccgcgc ggcctcgtca
    26101 tcctgaagga ttcgacgccg tagcttcgcc gtcgctgtcc ggccgctcgt cgtcgatcac
    26161 gagcttgagg cccgctggca gcgtatcgcc gagcgcgacg cgttcttcgc gtgcttcgag
    26221 cgccaccact tgctcgacga gcacgaccgc acgatcgcca ccgcggagat tgcgcaggaa
    26281 gctcgcgagc tgtgcgcgcg tcgcgtcgtc gagatcgcgt gtgcgatctc ccgtgcggcg
    26341 gccgagctgg gcgagcggaa acgcgaccgg cgagactccg ttcggatcgt cggccttgtc
    26401 gggccactcg ccctgcatga gcggtgcgag ccattccttc accttgttcg gcggaacgac
    26461 cgcgttcagc ggaccgtaga gcggcacccg cgcgccgatg cgagagagcg cccagaggtg
    26521 gacggtctcc tcgcggcctt tgcgcgtcgc catccgctgc acgagctcgt cgccgagctt
    26581 cactttgtgt tggatcggca ggcgctccat gctcgcgacg cagcgccaca tctcggcgag
    26641 ctcttgcttg ctcggcttcg tctcggccag cttcttcgcc tgcttcgtgc tcggcagcag
    26701 cagttgcgcg gtgcgatcgt agatctgatc ttgctggccc ttcgcgaggc caccggcaat
    26761 gcggcgccac gcgatccacc acgcgagctt gcccggctcg cttttggcgt gcgcgaggtt
    26821 ctcgttgaag acgccccaca tcacgcgcga gcgccacgcg tcgagcggcg cgccggtgcc
    26881 gggacggagc aggaagccgg cgaggttgag ccagcgctgc tcgtggtcgg cggtcttctt
    26941 gcgatcggcg gcgccgtcga tgagcgcgtc gaacaatgcg cgcgtcgtct gcatcgacca
    27001 ctcgtcgcgg cgtccttcga gcagcgtctc gagatcgcgc atgagcgttt gcagaccggc
    27061 gcccgtcgag aacgcggctt tcactttcgc tttcgcctct tcggccttcg ggtgcggtgc
    27121 ggcgtccgct gcatcttcgg gcgcgggcgc agcgccgccg gagcgcatgt cgaaggcgag
    27181 cttccaggtc tcgttgggcg gctccgtatc gacgcagtag atctcgagcg caccgagcgc
    27241 cgtgatgtgc acctcgagtc ggacggtcac ttcgccgcgg ccgcgcgcac gcagcaccgt
    27301 gacgatcggc ggaagctcga ggaggtcgct gccgtcgtcg atcgtttctg ccttgccgtc
    27361 gccgatcggc acgagcgcac ccggttgatc ttcgcgcgtg ctcgacgagt agagccggaa
    27421 gctcaccggt cggttcgtca cgagcttgaa gtcgcgagcg agctgcacgc gcgctccatc
    27481 atcgaggccc ggtggcgcga gacacaccgc aaatcgcgcg ggctcttcac tcttgccggc
    27541 ttccatcgtc ggaacgcggc ggcgcgagcc cgtggcatcg acaccgatgt agaacgcgcg
    27601 cggcgttccg cctttgatcc gcgtcgcgag accgcgacga acgaggccgt agtacgcagc
    27661 gccttgcgcg acggccatct cgggcatctg cgcctcgagc tcgcgcggcg gcgagccctg
    27721 ccaatcgccg atctgcttca gcacgcggtc gcgcaatgtc tgcggcgtca tcgcaccgcc
    27781 gttgaacagc accgcatcga tctgcgtcgc gttgtgccgc tgcaggaaca ccgcgagatg
    27841 gcgcgtgacc gcaggatcgg atgcgtaggg caggccgaac tcttgaaggc cgctgcgccc
    27901 gcgcgagagc tgcgcgtcct tcgcgacgaa tgggaagaaa ccgtcgaaca ggatcttctc
    27961 gagctcttcg cgcgagacct cgtcgcggat cgtcccgccg atgagctttg caccgcggct
    28021 ctgcacgacg atcggcgcgg tgtccggcgg atcatcaccg aggagcttct ccttcgcgag
    28081 cctgcacgcg tggacgagcc cgtgccactg cagtgcgtcg agcttcttgc cgctggtgtc
    28141 tttcacgcgc tgctcgacga tcttggcgag cgtcagatcc agattgtcgc cgccgagcag
    28201 caggtgatcg ccgaccgccg tgcgcgtgaa tccatcgcca cccggatcga cgtcgatcaa
    28261 cgtgaagtcc gtcgtgccgc cgccgacgtc gaagacgagc acgtgctcgc ccggcgcgag
    28321 cttgctgcgc gcggtgtcga tccacgcgta gaacgccgcc tgcggttctt cgagcaacac
    28381 gaccggcgga aaccccgcgg cgaccgaggc ctgcagcgtc agctcgcgcg cggcttcgtc
    28441 gaatgatgca ggcaccgtga cgagcacttc ctgctcttcg aacggcgcat cggcgtgctc
    28501 gtcgtcccac gcctcgcgaa tgtggcgcag cacctgcgct tgcgcgttga tcggcgagag
    28561 cttcgggccg tcgttgtctc cccacggcag gatcgccgcg ccgcgatcga cgcccgggtg
    28621 acacagccac gacttcgcgc tcgcgatcat gcgcgatgcc atgcgtgcac cctggctccg
    28681 agccagctcg ccgacgacgg gccgcagtgc cgtcgacacc gccgcctgcg gatcgacact
    28741 tttcgccggc gcggggcgcc acggcagcgc ggtctcgtgc ggcgccagat cgatatcgcc
    28801 ggcgaggtac acgaacgacg gcagctgccg cctcggtgcg acctcacccg gtgcgacgag
    28861 ctgaggcact tcgaacaccc tcacccgtgg atccgccgcc gccgtatcga cataggccac
    28921 cgccgagttg gtggtgccta agtcaattcc gaccaggtaa cgcgcgggca cgggcgggtc
    28981 ttaccatgcg aacatgcagc gacgcatgaa agccggggcc gtggtcgtcg tgtcgctgct
    29041 tctcgccgta ggccgggcgt atgcctccga tccgggcaac tcgtgcgagg acgaagcgag
    29101 cgagctccgc caggccctga cgcgcgagtc gagccgcgca gagacatgga acaccgtctg
    29161 gcgctggacg ttcacaggcg ccgccgtctc gagcgctgcg atcgcgtacg tcgacttcga
    29221 cttcttgcac gactcgcagg ccggcatcta cgtcagcgcg ggcaaagcat cgatcggagc
    29281 catcgcgcgc tggatcatgc ctcttcatat ccacgttccg ccgccgaccg ccgacgcctg
    29341 cgccgacgtc gaagcgctgc acaaagagat cacgcgcgtc gcgaagaaag agcgcggcct
    29401 gttcatcacc ggccacatcg gcggcctctt gctcaacggc gtcggcggct acgtcgtcta
    29461 tcactacagt ggccttggcc aagccgcgct ctcggtcggg ctcggctatc ccatcggcct
    29521 gctctcgaat tacacgatgc cgcgctcgag ctggcaccgc taccgcgatc gagaatggga
    29581 tcttccacca ccggcaccga tgccgacgct gagtgtgatc ccgacgcgcg gcggttggtt
    29641 cgtcagcctc ggcggagcgt tctaatgcat cgcctcgctc tcctcgcaat tctcgcggcg
    29701 tgcggacaac cgtcgcgacc cacgacatcg tcgccgtcgc aggttgcatc ggcaccgacc
    29761 ccgtactcgt tcgacttcct gcccgccgat ctgcgcgagc gcggcccatc gctcatcgac
    29821 atccaatgcc ccgcaggcac gaagcgcgtc gagacgagca gctacggccg cacgcatcgc
    29881 cattgcgaaa cgccgaccaa agaatacgaa ggcccgatcg tcgaattcgc cgagggcgag
    29941 atcaggctgc gtggcacgca cgctggctat cgctccggca tcgagatctc cgccaacgcc
    30001 ggcggtgtca tcgaagcgga gtacgcggca aatcacccgg tcgggcgcta ccgctatcgg
    30061 aaaggctcga tcgtcgtcgc cgaaggggcg ttcgacagcg aggggcgcct cgacggcgag
    30121 tggatctttc gcacgcccgt cagcgggcaa gagctcaccc gcacgacgtt cacgcatggc
    30181 acgggctcga tcgaaatctg ggattggcgc gggtcgcagc cgaaggtggc cgcgcgcctg
    30241 caatgcaaag acgggctgct cgacggcgcg cagatgttcc acct
//
LOCUS       DATLBZ010000616        16503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10948279, whole genome shotgun sequence.
ACCESSION   DATLBZ010000616 DATLBZ010000000
VERSION     DATLBZ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16503
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10948279"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /gene="fghA"
                     /locus_tag="VMZ53_30255"
     CDS             complement(<1..694)
                     /gene="fghA"
                     /locus_tag="VMZ53_30255"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR02821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HUS32837.1"
                     /translation="MRTRSEHSSFGGTQGFYEIDSAACGGPMRFGVYIPPNAKAAPAL
                     YYLAGLTCTEETFAMKAGAQRVASELGLAIVTCDTSPRHARFPGDDADWDFGLGAGFY
                     LDATQAPWSAAYKMETHVVDELRAAVEGAFPIARDRRGITGHSMGGHGALTLALRHAD
                     LYRSASAFAPVCAPMQVPWGQKAFRNYLATDGADHDACALITAGKRFTTPPLVDQGLA
                     DEFLETQLRPDLL"
     gene            complement(694..1803)
                     /locus_tag="VMZ53_30260"
     CDS             complement(694..1803)
                     /locus_tag="VMZ53_30260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HUS32838.1"
                     /translation="MKTRAAIAMEVGKPLVVDELDLEGPRAGEVLVEIMATGVCHTDE
                     FTRSGADPEGLFPVVFGHEGAGIVREVGAGVTTLKPGDHVIPLYTPECRTCKSCLSRK
                     TNLCTAIRATQGKGLLPDGTSRFSYKGKPVFHYMGCSTFSNFTVLPEIALAKVRDDAP
                     FDKICYIGCGVTTGIGAVLFTAKVEAGANVVVFGLGGIGLNVVQAARMAGADMIVGVD
                     INPKREAMARTLGLTHFVNPRDIKGDLVAHLVELTNGGADYSFECVGNVELMRQALEC
                     CHRGWGVSVIIGVAGAGQLIQTRPFQLVTGRVWKGSAFGGARGRTDVPRIVDWYMNQK
                     IQIDPMITHKLPLERINEAFDLMHEGTSIRTVVEF"
     gene            complement(1853..2167)
                     /locus_tag="VMZ53_30265"
     CDS             complement(1853..2167)
                     /locus_tag="VMZ53_30265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32839.1"
                     /translation="MDGCGHPLCELLTGPCSLATGRSRGCTPGLAPKTVGPEVFDEVR
                     ALLAVIDPVNGHGRDAARRLAEIIRREARDDRNLVTQARDEEPELAVALERLCLRRSS
                     RA"
     gene            complement(2177..3256)
                     /locus_tag="VMZ53_30270"
     CDS             complement(2177..3256)
                     /locus_tag="VMZ53_30270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32840.1"
                     /translation="MAEQGVTNEGRRLFEHETFGGNGRTCRTCHSPANGTLTLTQIAE
                     RFAQDPNGPLFRGDGTDDGRGNGTARIRADGTILVSVALPPGVTVLDDPTADRVTLRR
                     GIPSTNNTPALDDVLMYDGRAPDLIEQARGAIHDHAANTVAPTDEQLELIAEFQQSPQ
                     FFTSNALLAFANGGPPPQLPEGNTASEKRGRRFFDNVPLSPPSTRGICAICHSGPMLN
                     ESNGKNPIPIPPFFVPQGERFQSILSGEFLPNGDAFRAYQFTDPDGVIQVSVASDPGR
                     AATTGDFRGFPFGNLGQFKIPSLWNVRNTAPYFHNSGAKTLEQVIEHYSVFFPIATPV
                     AMPGAPPIVFTEQDKADLLAFLKLL"
     gene            3509..4435
                     /locus_tag="VMZ53_30275"
     CDS             3509..4435
                     /locus_tag="VMZ53_30275"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS32841.1"
                     /translation="MRFDLDDRHVARWRDEVCDQMLDADSPWDATKRLGVQFGVTAIR
                     GVVQLDAHAKEPGLVIERSGTRVAQDKRPATMGLTLVTAGETSIITRGREVVVRAGEL
                     CLLSSLEPFRKHMSADYREHFLYLPVPVALALGRPVPTLPEHRLVAPQRGLGSVLADT
                     ILSLSRTRNELDMTDWETALGAVFELAAGVFGRRDRELLQAPTRQAQRARAIRYIEAH
                     LADSQLAPPSIAEGLGVSIRYLHLLFEESESVGATILARRLDRCRAALADAAERRSIS
                     EIAFAWGFNDAAHFSRTFRSRFGMSPRDARGR"
     gene            4453..5445
                     /locus_tag="VMZ53_30280"
     CDS             4453..5445
                     /locus_tag="VMZ53_30280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HUS32842.1"
                     /translation="MRSVVAKRYGGPEMLDLVERDEPSVGARDVLIDVKAASLNPLDF
                     KIRDGKTKLVLPLKPPIALGCDVAGVVTAVGGSVTRFKVGDDVFARLEKMRMGGLSER
                     VAADENVVAMKPARVSFVEAAAIPLAALTALQAFREVAAVQPGQRVLIHAGAGGVGSL
                     AIQIAKVLGVHVITTTSAKNADFVKSLGADEVVDYTHERVVDRVRDVDAVFDTLGTTE
                     IESLAVVKRGGVVVGIGGLPDGAFAREWLPWWTPPAIWMMTRKRRAAAARHGARFVYW
                     FMRPDGEQLAELARWIDDGKLQPIVHKEYPLAEFREAFAELERGRSRGKIVLTF"
     gene            complement(5463..5921)
                     /locus_tag="VMZ53_30285"
     CDS             complement(5463..5921)
                     /locus_tag="VMZ53_30285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32843.1"
                     /translation="MERGIANILHRDTIAVIVGAHMFERGKKCFAEARVQEVAASGGE
                     LRGRVRPSESGRAPYTVRIWVRDEGLAYECTCPIGAKLQFCKHTVAIALAHLEAERVE
                     AERGLGVLREALQTIPPAALVDGLLVMARRDPGLSDDLKRMCLDVLSGSR"
     gene            6319..8124
                     /locus_tag="VMZ53_30290"
     CDS             6319..8124
                     /locus_tag="VMZ53_30290"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HUS32844.1"
                     /translation="MPTAPSKAPSSASCADEPIVVFDGGREGDHVCATPAKATLVDLR
                     DAWAPSLFAVQADGTAPEYRATYLAAALEQDASGKKLPASVGLSELYGVLPSFAIVRD
                     RFTQTARYACHAKIDSTPIAKIARPYGQELDALVRFNNRQRELLGKILERARTQRGAA
                     DFTVFADDKELGDTYARWKTADELYSGIVAAQQHLVCEGELDDKAVDGEFSWVFGQAL
                     EHFQRRNFLIPNGRLDPETRAAFATDPRELDYRFALRILRERIVDATGLIEDGTAGAG
                     PQPILGRMLDPEAMRRVNGNTKAMANAAPDLVSATTEAAAKQLGWTDPDATAAWLAKH
                     TGGLRVAMELPALPSYYSNHMDLSAEIDRGDVWYDEKPVPRVAWKRPALVVYVQDGTT
                     KRPLVRWPTTIGGWSDVRIGGVLQKRWKESDVGPREWRAIYAAPTWLPPPATPDEELV
                     RWKGKGKWELKTSIMGPGPLSAFGMIRLPHERAVKTKGGATQWIDNGIGTHGSAVVTS
                     LLNGTSHGCHRLYNHLAIRLGGFLLDHRNHEVKGEQPEHYRRVVNAGGKFTAKIDTRG
                     FMYELTPPVHVEVLPGNIRSPRKVPPAASVPAGAE"
     gene            complement(8125..8538)
                     /locus_tag="VMZ53_30295"
     CDS             complement(8125..8538)
                     /locus_tag="VMZ53_30295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32845.1"
                     /translation="MKTAQRGALVGAATMVVITMIWTAMAGSPGEGTCAWSRACLCQP
                     APSSAFLVGGLVVGVPWGAIVGGFAATLASRFARHRRLILVGFALVGASVVEAIARRF
                     LHCSTDPDTLALWSSAVVAMVLGALSLARSIDLGE"
     gene            8674..8976
                     /locus_tag="VMZ53_30300"
     CDS             8674..8976
                     /locus_tag="VMZ53_30300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32846.1"
                     /translation="MALALQLRPPMTILDNSTLAAVRGGDGEPQPAQPAQPAKRSWNQ
                     FAADYVASCATTATNAAVFQGRPGSWQQLAITGAIGCAAGIATQAAQDWRARASAK"
     gene            8989..10233
                     /locus_tag="VMZ53_30305"
     CDS             8989..10233
                     /locus_tag="VMZ53_30305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS32847.1"
                     /translation="MQSPLRTAAAGLIGNVLEWFDYAVYGYFSTEIGAQFFPASMSKY
                     DKSLFTFAIFWLGFAARPIGGLVLGVVGDRIGRRALLTLSIALMGGATLVIGLLPTYQ
                     SIGIAAPLLLVAMRLVQGFSLGGEFTGSMVYTTELASPLLRGIVASSTAAGTTLGFIL
                     GSLTAYLVNRGLGHEAAADWGWRIPFIGSLVFCVVGFALRSGIHESEAGQKAAAERAP
                     IVQSLIADWKPIVQTFGIVAMTNAAYYLAFTFAVERRKAEDTDFQLANTISLCVVLVF
                     KVLGGWLSDRVGRRRLMIALTFVLIGTLYVGQRLMLYGDPPIFMVGQLMLGIPLAMAL
                     GLQGAMVVEIFPLRTRVTSMSFAYSITLALAGGLAPIVATYLIDRVGEPIAPAFYVMA
                     YGVVGLPIMFGMRETNQRSLDD"
     gene            10251..10991
                     /locus_tag="VMZ53_30310"
     CDS             10251..10991
                     /locus_tag="VMZ53_30310"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS32848.1"
                     /translation="MNRLLVIEDETDSLEMLQLLLQSAGFEVVGVSTRAEAIRVLQQQ
                     AFDLVLADLMIDSREIAVAWTAVDELVALARPALVGLITGWPVPEEEAAAHHVAFVLQ
                     KPCSRDAMFRQLAAALQLPALTVAQAFAVNRYFEALELREYDQLSEIVTDDVVYQLPG
                     TDARFSQEIRGRDAFLEFTNKTFNGFDRAHFEVNAIRSLPNGAMVEYVGHWKEANRER
                     ALPGAVMFELRDDRISRIAVRVNPDRLV"
     gene            11118..12038
                     /locus_tag="VMZ53_30315"
     CDS             11118..12038
                     /locus_tag="VMZ53_30315"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HUS32849.1"
                     /translation="MKTWSVCLLVSLCGASACIGSEDDENIDVTVGDEKADAVSHVTV
                     NAGSTSTVNFTATAGAVDITVDCAPPANPDTVGTQFTVKGTGLAGLSSASAPIAGLWQ
                     WSGTVTAGTKKLELKGVSGSRSCTVRVRKATGQCTSSTVTRSPETGHTHLFVNSNLTT
                     APFPASGNHWGAWAKWGTIYSKPVKRGFYLHNLEHGGLVLSYKCTSSTASAECADAAA
                     KMTALANSFGEYRVIVTPDPQQPTLFGIRGWRVGYQSDCFNEQRMLDFMGDHFRNGRE
                     DTDADPPVPYDPTTSQVPCVDLMAAPDSCN"
     gene            12048..12848
                     /locus_tag="VMZ53_30320"
     CDS             12048..12848
                     /locus_tag="VMZ53_30320"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HUS32850.1"
                     /translation="MRVAVLGHVEWVEFLRVPAVPHAGDIVEGVPLVAVPAGGGGVAA
                     VQLARWARSCDLFTALGDDELGHRCEAQLRARGVNVHAAWRAEPQRRAVTLIDAQRER
                     TIILSGHRHIAKRADALPWDAFGDIDTVYVTGGDADAVRAARAAKVVVSTARVLPLLR
                     DAAITLDALVGSDNDPAEIYPGDLHPSPRLVVRTNGAMGGMYTLAGGAPQAYAAIQTT
                     VVGDTYGAGDTFAAAIAFGLGEGRQPGDAIAFAAARAAEVLAFHGPYG"
     gene            12862..14544
                     /locus_tag="VMZ53_30325"
     CDS             12862..14544
                     /locus_tag="VMZ53_30325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HUS32851.1"
                     /translation="MSTEPEQLPMGTKIATMVGALLGLFLAALDQTIVSTAGPFIQKD
                     LSIEPGIYVWITTAYLVASTVLVPIYGKLSDLYGRRRILLVAIGIFLAGSLACGASQT
                     AAQLIASRAVQGVGSAGLFTSAFAIAADLWAPAERGKWQGIFGAAWGVSSVIGPLLGG
                     VLTESLSWHWTFFVNLPIGAVAVSLIMSKMPPLRREQTTKPTLDIAGAVALAVGTLPL
                     LLGLSLGHAEGTGQTTGWPWKSWQIAALFATSAAGIIAFILIELRAKNPILDFKLFRI
                     KTFAIGSLATFVVGSAFLGAIVFLPLFMVNVVGLSATSSGLTTMPLTFGIVVANIASG
                     QLVSRLGAYKGLLLASLVVLCASFLWFALTLDIDATRASISMKMVFLGIGLGPSIPLF
                     TLAIQTSVPMQQIGVATASATFFRQMGTTIGIAIIGTIFATSFAGALNREMPKPEEMP
                     PQMRAMMERKGGGGGEGGARVAFDAKAARARADAGIDSSPMPDFAKAQAKENAHVAIS
                     KVDHAFKRGFTNALINVFWVSLAIAIAGLLITVFLPQLPLRKGFGAPPPPAE"
     gene            complement(14545..14742)
                     /locus_tag="VMZ53_30330"
     CDS             complement(14545..14742)
                     /locus_tag="VMZ53_30330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32852.1"
                     /translation="MKEVFRGASIVVYATSRRLDDVPGGASWYWYEGIGDDVSSIGQD
                     VESCIGCHAGASRDFVFTVVR"
     gene            15042..16133
                     /locus_tag="VMZ53_30335"
     CDS             15042..16133
                     /locus_tag="VMZ53_30335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32853.1"
                     /translation="MSTGIEVPWSRLLGIAGALLALRTQACEPTRTMPATIATDGNAL
                     LVAWEKPTDDGFTIYMRHVPDVQDGTTTLQGGTAIYTKKYGTTGYHGPEVATGPAGSI
                     VIITPRVGEQIAIPVDLDGRVAGPAQPLTGCRSDSSDRTCFWSCRRPIAHAGGFIVGH
                     VSAYPRSSIHSLDLSFLDRAGRTEKFLVLRSQDPVACAMAASGDELVVVSTERETLVD
                     VKIHVLFLALSDGELLNELWIDGGYAFGIVSTSANEYALLHQIPDGRSVLTKFNRKGI
                     ESTHLVPEAIDLRTADLGMSFRGLFVSWLANGRAHVLDLTTDREVSRKGVARYSVGTR
                     AVGVRDRCVTAWTSGAGNKVQLLAVPECP"
     gene            complement(16134..>16503)
                     /locus_tag="VMZ53_30340"
     CDS             complement(16134..>16503)
                     /locus_tag="VMZ53_30340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32854.1"
                     /translation="SRHFSRPAAGDPADFDRARAHYETIPESSVPFASFRKYAGLAYC
                     AWRQGNDDEARRLAERAADDAGDGGLVRMRIQALNMLSRVVPNEQAQAVNARARSMAA
                     ALEDENLLRRVNLCTPRRAS"
BASE COUNT         2699 a         5492 c         5461 g         2851 t
ORIGIN      
        1 cgagtagatc ggggcggagc tgcgtctcga ggaactcgtc ggcgaggcct tgatcgacga
       61 gtggcggggt cgtgaagcgc ttgcccgcgg tgatcagcgc gcacgcgtcg tggtctgcgc
      121 cgtccgtcgc gaggtagttg cgaaatgcct tttggcccca tggcacctgc atcggcgcgc
      181 acaccggcgc gaaggccgac gccgagcgat agaggtccgc gtggcgcagc gccaacgtca
      241 gcgcgccgtg ccctcccatc gagtggcccg tgatgccgcg ccggtcgcgg gcgatcggga
      301 atgcgccttc gacggcagca cgcagctcgt cgacgacgtg cgtctccatc ttgtacgcgg
      361 cggaccacgg cgcttgcgtc gcatcgagat aaaagcccgc gccgaggccg aagtcccagt
      421 ctgcatcgtc gccgggaaag cgcgcgtggc gagggctcgt atcacacgtc acgatcgcga
      481 ggccgagctc cgaggcgacg cgctgcgcgc cggccttcat cgcgaacgtc tcctcggtgc
      541 acgtgaggcc tgcgaggtag tagagggccg gcgccgcctt cgcgttcggc gggatgtaca
      601 cgccgaagcg catcggtccg ccacatgcgg cgctgtcgat ctcgtagaag ccttgcgtgc
      661 cgccgaacga ggaatgctcg gagcgcgttc gcatcagaat tcgaccacgg tccggatcga
      721 tgtgccttcg tgcatcaggt cgaatgcttc gttgatgcgc tcgagcggca gcttgtgcgt
      781 gatcatcgga tcaatctgga tcttctggtt catgtaccag tcgacgatgc gcggcacgtc
      841 cgtgcgaccg cgtgcaccgc cgaacgcgct gcctttccac acgcgacccg tgacgagctg
      901 gaagggccgc gtttggatca gctggcccgc acctgcgacg ccgatgatga cgctcacgcc
      961 ccagccgcga tgacagcact cgagcgcctg gcgcatcagc tcgacattgc cgacgcactc
     1021 gaagctgtag tccgcgccac cgttcgtcag ctcgacgagg tgcgcgacga ggtcgccctt
     1081 gatgtcgcgc gggttcacga agtgcgtgag gccgagcgtg cgcgccatcg cttcgcgctt
     1141 cgggttgatg tcgacgccga cgatcatgtc ggcaccggcc atgcgcgcgg cttgcaccac
     1201 gttgagtccg ataccgccga ggccgaacac gacgacgttc gcgccggctt cgaccttcgc
     1261 ggtgaagagc accgcgccga tgcccgtcgt gacgccgcag ccgatgtagc aaatcttgtc
     1321 gaacggcgcg tcgtctcgca ccttcgcgag cgcgatctcc ggcagcacgg tgaagttcga
     1381 gaacgtcgag cagcccatgt aatggaagac gggcttgccc ttgtacgaga agcgcgacgt
     1441 gccgtccgga agcaggccct tgccctgcgt tgcgcggatc gccgtgcaga ggttcgtctt
     1501 gcgagacagg cacgatttgc aggtgcggca ctcgggcgtg tacagcggga tcacgtggtc
     1561 gcccggcttc agcgtcgtta cgcctgcgcc gacttcgcgc acgatgccgg cgccttcgtg
     1621 gccgaacacg accgggaaga ggccttccgg atcggcgccc gagcgcgtga actcgtcggt
     1681 gtggcagacg cccgtcgcca tgatctcgac gaggacttcg cccgcgcgcg gcccctcgag
     1741 gtcgagctcg tcgaccacta gcggtttgcc gacttccatg gcgatcgcgg ctctcgtctt
     1801 catgggccga gcatgcccgg aagcgcgcac gagcgaaaca cgcacgccgc tgttacgcgc
     1861 gcgagctgcg acgaagacag agacgctcga gcgcaacggc cagctcgggt tcttcatcac
     1921 gtgcttgcgt gacgaggttt cggtcgtcgc gcgcttcacg gcgaatgatt tcagcgagcc
     1981 ggcgagcggc gtcgcggcca tgcccgttca cgggatcgat cacggcaagc agcgcgcgga
     2041 cctcgtcgaa cacttcaggc ccgaccgtct tcggcgcgag gccgggcgtg caaccgcgcg
     2101 agcgtcccgt cgcgagactg catggtccgg tgagcagctc gcacagcgga tgcccgcagc
     2161 cgtccatgca tcgagtctag agcaacttca ggaacgcgag caggtcggct ttgtcctgtt
     2221 cggtgaagac gatcggtggc gcgcccggca tcgctaccgg cgtcgcgatc gggaagaaca
     2281 ccgagtagtg ctcgatgacc tgttcgagcg tcttcgcgcc gctgttgtgg aagtacggcg
     2341 ccgtgttgcg cacgttccac aggctcggga tcttgaactg gccgaggttg ccgaatggga
     2401 agccgcggaa gtcgcccgtc gtcgccgctc gtccaggatc cgacgcgacg gagacctgga
     2461 tcacgccatc gggatcggtg aactgatacg cgcggaatgc gtcgccgttc ggcagaaact
     2521 cgccactcaa gatcgattgg aagcgttcgc cttgcggcac gaagaacggc gggatcggga
     2581 tcgggttctt gccgttcgat tcattgagca tcggcccgct gtggcagatc gcgcagatgc
     2641 ctcgcgtcga cggcggggac agcggaacgt tgtcgaagaa ccggcgcccg cgcttctccg
     2701 aagcagtgtt gccttcgggc agctgcggcg gtggaccgcc attggcgaat gcgagtaacg
     2761 cgttcgacgt gaagaactgc ggcgactgct ggaactcggc gatgagctcg agctgttcgt
     2821 ccgtcggcgc aaccgtgttc gcagcgtgat cgtggatcgc gccacgtgcc tgctcgatca
     2881 gatcgggcgc gcggccgtcg tacatgagca cgtcatcgag agctggcgtg ttgttcgtcg
     2941 acggaatgcc acgccgcagc gtgactcgat cggccgtcgg atcatcgagc acggttacgc
     3001 cgggcggcag cgcgacgctg accaggatcg tgccatcggc gcggatgcgt gccgtcccgt
     3061 tgccgcggcc gtcatccgtg ccgtcgccgc gaaacagcgg gccgttggga tcctgcgcga
     3121 accgctcggc gatctgcgtt agcgtcagcg tgccgttcgc cgggctgtgg cacgtgcggc
     3181 acgtgcggcc gttgcccccg aatgtctcgt gctcgaacag gcgccggcct tcattggtga
     3241 cgccctgctc tgccaccctc gtttgtggct cgctcgccac gcaacccatg accaacatcc
     3301 ccactgcaat tccaagcgtt cgcatgcatg tcctcctcgg gccacgatgc cgaacggcac
     3361 agggcgcggc ttgctcggtc gtgcaccccg gcttgtctct gcgtgcggac gtgccgccgc
     3421 gtgcacgacg acttgtctga gagtgcaatc gcgtcggacg cgcgcgcgcg gtgatattct
     3481 gggtgcggtg gacggtgcgc tcacatcggt gaggtttgat ctcgacgatc gacatgtcgc
     3541 gaggtggcgt gacgaggtct gcgatcaaat gctcgacgca gactcgccat gggatgcgac
     3601 gaagcggctc ggcgtgcagt ttggcgtgac ggcgatccga ggcgtggttc agctcgatgc
     3661 tcacgcgaag gaacccggtc tcgtgatcga acgctccggg acgcgcgttg cacaggacaa
     3721 gcgccccgcc acgatggggc tgacgctcgt cacggcgggc gagacgtcga tcatcacgcg
     3781 cgggcgcgag gttgtcgtgc gtgcaggcga gctgtgtttg ttgtcgtcgc tcgaaccatt
     3841 tcgcaagcac atgtccgcgg actaccgcga gcacttcctc tatctcccgg tccccgtcgc
     3901 actcgcgctc ggccggcccg ttccaacgct gcccgagcac cggctcgtag ccccgcaacg
     3961 cggacttggc tcggtgctcg ctgacacgat cctctcgcta tcgcgcaccc gcaacgagct
     4021 cgacatgacc gactgggaga ccgcgctcgg cgccgtgttc gagctcgccg ctggtgtatt
     4081 cggccgccgc gaccgcgagc tgctgcaagc gccgacgcgt caggcgcagc gcgcgcgagc
     4141 gattcgctac atcgaggcgc accttgcaga ctcgcagctc gcgccgccgt cgatcgcgga
     4201 aggcctcggc gtgtcgatcc gatatctcca cttgctgttc gaagagagcg agtccgtagg
     4261 cgcgacgatc ctcgcgcgtc gcctcgatcg ctgtcgtgcc gcgctcgccg atgcagcgga
     4321 gcgacgctcg atcagtgaaa tcgcgtttgc gtggggcttc aacgacgccg cgcacttctc
     4381 gcgcacgttt cggtcgcgat tcggcatgtc cccgcgcgac gcacgcgggc gctagctcgt
     4441 aagctcctca gcatgcgtag cgtcgtcgcc aagcgatacg gaggccccga gatgctcgat
     4501 ctcgtcgagc gagacgagcc ctccgtcggc gcgcgcgatg tcctcatcga cgtcaaggcc
     4561 gcgagcttga acccgctcga cttcaagatc cgcgacggca agacgaagct cgtgctgccg
     4621 ctgaagccgc cgatcgcgct cggctgcgac gtcgcgggtg tcgtgaccgc tgtcggcggc
     4681 agcgtgacgc gcttcaaagt gggcgacgac gtattcgcgc gactcgagaa gatgcggatg
     4741 ggcggcctct ccgaacgcgt tgccgccgac gagaacgtcg tcgcgatgaa gccggcgcgc
     4801 gtgagcttcg tcgaagcagc cgcgatcccg ctcgccgcac tcacggcgct ccaagcgttt
     4861 cgcgaagtcg ctgccgtgca gcctggccag cgcgtcctca ttcacgcggg tgcaggcggc
     4921 gtcggcagtc tcgcgatcca gattgcaaaa gtgctcgggg ttcacgtgat cacgacaacg
     4981 agtgcgaaga atgcagactt cgtgaagagt ctcggcgcgg acgaggtcgt cgattacacg
     5041 cacgagcgcg tggtcgatcg cgtgcgtgac gtcgacgccg tcttcgatac gctcggcacg
     5101 acggagatcg agtcgctcgc ggtcgtcaag cgcggcggtg tcgtcgtcgg catcggtggt
     5161 ttgcccgatg gtgcgtttgc tcgcgagtgg ctgccgtggt ggacgccgcc ggcgatctgg
     5221 atgatgacga ggaagcggcg cgcagcggcc gcgcgacatg gcgcacggtt cgtctactgg
     5281 ttcatgcggc cggacggcga gcagctcgcc gagctcgcgc gctggatcga cgatggaaag
     5341 ctccagccaa tcgtgcacaa ggagtatccg ctcgccgagt ttcgtgaagc gtttgccgag
     5401 ctcgaacgcg ggcgctcacg aggcaagatc gtcctgacgt tctgactgcg gccgcgatcg
     5461 attcagcgac tgccagacag cacgtcgagg cacatgcgct tcagatcgtc ggagagccca
     5521 gggtcccggc gcgccatcac gagcaagcca tcgaccagtg cggccggcgg gatcgtctga
     5581 agcgcttcgc gcaggacgcc gagacctcgc tccgcttcga ctcgttcggc ctcgaggtga
     5641 gcgagcgcga tcgcgaccgt gtgcttgcag aactgcagct tcgcgccgat cgggcaggtg
     5701 cattcgtagg ccaggccttc gtcgcggacc cagatgcgga cggtgtaagg cgcccgcccc
     5761 gattcgctgg gcctgacccg gccgcggagc tcgccgcccg acgctgccac ctcctggacc
     5821 cgcgcctcgg cgaagcactt cttgccgcgc tcgaacatgt gagcgccgac gatgaccgcg
     5881 atcgtgtcgc gatgcaggat gttcgcgatt cccctctcca cggtgctgat agtacgagag
     5941 tttcggaagg cctacatgaa tgggaagagc ggtcgatatc atctgcatgc ctcgccctcg
     6001 cccggacaca ctgaacaacc gtgagcccgc tccgccgccg gcgactccct ccgaggaggc
     6061 gcagcaggac ggcgcgaccg aggacgagcg cgtggtcttc tcctcctggg actgcccgtt
     6121 ggtgcccccg cttactcgcc gcgagtaggg agaacgccgg gctgacgcgg ctcgccacag
     6181 ccggtcgtcc gggcttcgga cagtttctcg acgagtcgcg gatcgcgcgc gatactgacg
     6241 gtgatgtacc agcggtgggt actcgcatgc ctgttggcag cgagttgttc ctcgtccgat
     6301 cgaaaaccag ttggcctcgt gccgacggcg ccgtcgaaag ctccgtcgag cgcgagctgt
     6361 gccgatgagc cgatcgtcgt gttcgacggc ggtcgcgaag gcgatcacgt gtgcgcgacg
     6421 cccgcgaagg cgacgctcgt cgatttgcgc gatgcgtggg cgccgagcct gttcgccgtg
     6481 caggcagacg gcaccgcacc ggagtaccgc gctacgtatc tcgcggccgc gctcgaacaa
     6541 gatgccagcg gcaagaagct gccggcgagc gtcgggctct ccgagctgta cggcgtgttg
     6601 ccgtcgttcg cgatcgtccg cgaccggttc acgcagacgg cgcgctacgc gtgtcacgcg
     6661 aagatcgact cgacgccgat cgcgaagatc gcgaggccgt atggccagga gctcgacgcg
     6721 ctcgtgcgct tcaataaccg ccagcgcgag ctgctcggca agatcctcga gcgagcgcga
     6781 acgcagcgcg gggccgcgga cttcaccgtc ttcgcggatg acaaggagct gggtgatacg
     6841 tacgcgcggt ggaagaccgc cgacgaactg tacagcggca tcgtcgcggc gcagcagcac
     6901 ctcgtgtgcg aaggcgagct cgacgacaag gccgtcgacg gcgagttctc gtgggtgttc
     6961 ggccaagcgc tcgagcactt ccagcgccgc aacttcctca ttcccaatgg ccgcctcgat
     7021 ccagagacgc gtgcagcatt cgcgacggat ccacgcgagc tcgactatcg gttcgcattg
     7081 cgcatcctgc gcgagcgcat cgtcgacgcg accggactta tcgaagacgg aacggcaggc
     7141 gccggcccgc aaccgatcct cgggcgcatg ctcgaccccg aggcgatgcg tcgcgtgaac
     7201 gggaacacga aagcgatggc aaacgctgcg cccgatctcg tgagcgcgac aacggaagcc
     7261 gccgccaagc agctcggctg gaccgaccct gatgcgaccg cggcgtggct cgcgaagcac
     7321 accggcggtc tgcgcgtggc gatggagctg cctgccctgc cctcgtatta ctcgaatcac
     7381 atggatctct ccgccgagat cgatcgcggc gacgtctggt acgacgagaa gccggtgccg
     7441 cgcgtcgcat ggaagcggcc cgcgctcgtg gtctacgtgc aggacggcac gacgaagcgc
     7501 ccgctcgtgc gatggccgac gacgatcggc ggctggtcag acgtgcgcat cggcggcgtc
     7561 ctgcagaagc gctggaaaga atcggacgtc ggtccgcgcg agtggcgagc gatctatgcc
     7621 gcgccgacgt ggctgccgcc gcccgcgacg cccgacgagg agctcgtccg ctggaaaggc
     7681 aaaggcaagt gggagctcaa gacgtcgatc atggggccgg gcccgctatc ggcgttcggc
     7741 atgatccgcc tgccgcacga acgcgcggtg aagacgaaag gcggcgcgac gcagtggatc
     7801 gacaacggca tcggcacgca tggctcggca gtcgtcacgt cgctgctcaa cggcacgagc
     7861 cacggttgcc atcgcctcta taaccacttg gcgatccgcc tcggcggctt cctgctcgat
     7921 catcgcaacc acgaggtgaa gggcgagcag cccgagcact accgccgcgt cgtcaatgcc
     7981 ggcggaaagt tcaccgcgaa gatcgatacg cgcggcttca tgtacgagct aacgccgccg
     8041 gtccacgtcg aagtcctgcc cgggaacatc cgctcgcccc gcaaggtccc gcccgccgcg
     8101 tccgtccccg ccggcgccga gtagctactc gccgaggtcg atcgatcgcg cgagtgacag
     8161 cgcgccgagc accatcgcaa cgaccgcgga gctccacagc gcgagcgtgt ccggatccgt
     8221 cgagcagtgt aggaagcgcc gcgcgatcgc ctcgacgacc gacgcgccga cgagcgcgaa
     8281 gccaacgagg atcagccggc gatgccgcgc gaaccggctc gccaacgtcg ccgcgaaccc
     8341 tccgacgatc gcgccccacg gcacgccaac gacgaggccg ccgacgagga atgcgctcga
     8401 cggcgcgggc tgacagaggc acgcacgact ccaggcacat gttccttcgc cgggactgcc
     8461 cgccatcgcg gtccagatca tcgtgatgac gaccatcgtc gcggcgccga caagcgctcc
     8521 tcgttgcgcg gtcttcacac cggcacaacg ctcgacatct cgcgtccatt cgcgacacgt
     8581 gtctaccgag tgccgcagcg gcgaggggca atgtcccagt cgccgagcaa ctcgagtggc
     8641 cgcggtctcg cgagcgattg cgcatagatc gcgatggcac tcgcgctgca gcttcgcccg
     8701 cccatgacga tcctcgacaa cagcactctt gccgcagttc gcggcggcga tggcgagccc
     8761 caacccgcgc agcctgcgca accggcgaaa cgcagctgga accagttcgc ggcggactac
     8821 gtcgcctcgt gcgccacgac cgcgacgaac gcggctgtct tccaaggccg gcctggctcg
     8881 tggcagcagc tcgcgatcac gggcgcgatc ggttgcgccg ccggcatcgc aacacaagcc
     8941 gcacaggact ggcgagcgcg cgcttccgcg aagtgagagt atcgcggcgt gcaatcgccg
     9001 cttcggactg ctgccgccgg cctcatcggc aacgtgctcg agtggttcga ctacgcagtc
     9061 tacggctact tctcgacgga aatcggcgcg cagttttttc ccgcgtcgat gagcaagtac
     9121 gacaagagcc tgttcacgtt cgcgatcttc tggctcggct ttgcggctcg tccgatcggt
     9181 ggcctcgtgc tcggcgtcgt cggcgatcgc atcggccggc gcgcactgtt gacgctctcg
     9241 atcgcactga tgggcggcgc gacgctcgtc atcggcctgt tgcccacgta ccagtcgatc
     9301 ggtatcgctg cgccactctt gctcgtcgcg atgcgcctcg tgcaggggtt ctcgctcggc
     9361 ggcgagttca ccggctcgat ggtctacacg accgagctgg catcgcccct gctccgcggc
     9421 attgtcgcga gctcgaccgc cgcgggcacg acgctcggct tcatcctcgg ctctctgact
     9481 gcatacctcg tcaaccgcgg gctcggtcac gaagccgcag cggactgggg atggcgcatc
     9541 ccgtttatcg gcagcctcgt gttctgcgtg gtcggctttg cgctgcgcag cggcatccat
     9601 gaatccgagg ccggccagaa agcagcagcc gagcgcgcgc cgatcgtgca gtcgctgatc
     9661 gcggactgga aaccgatcgt gcagacgttc gggatcgtcg cgatgacgaa tgctgcgtac
     9721 tacctcgcgt tcacgttcgc ggtcgaacgt cgcaaagccg aggacaccga cttccagcta
     9781 gcgaacacga tcagcctgtg cgttgtcctc gtgttcaagg tgctcggagg ctggctatcc
     9841 gatcgggtgg gtcgtcgacg gctcatgatc gcgctgacgt tcgtgctcat aggaacgctc
     9901 tatgtcgggc agcggctgat gttgtacgga gacccaccga tattcatggt ggggcaactc
     9961 atgctcggca ttcctctcgc gatggcgctc ggccttcaag gcgcgatggt cgtcgagatc
    10021 ttcccgctgc gcacgcgcgt gacatcgatg agcttcgcgt actccatcac gctcgcgctc
    10081 gctggcggcc tcgcaccgat cgtggcgacg tatctgatcg accgcgtcgg agagccgatc
    10141 gctcccgcgt tctacgtcat ggcctatggc gtggtcggtc tgccgatcat gttcgggatg
    10201 cgagagacaa atcagcggtc tctcgacgac taggcgtgct acacagcacc atgaaccggc
    10261 tgctggtgat cgaggacgag acggattccc tcgagatgct gcagctgctc ctgcagagcg
    10321 ctggcttcga agtcgtcggt gtgagcacgc gagcggaagc gatccgcgtg ctgcagcagc
    10381 aagcattcga tctggtgctg gcagatctga tgatcgactc gcgagagatc gcggtcgcgt
    10441 ggaccgccgt cgacgagctc gtcgcgctcg cgcgccctgc gctggtcggt ttgatcaccg
    10501 gctggcccgt tccggaggaa gaagccgctg cgcaccacgt cgcgttcgtg ctgcagaagc
    10561 cctgttcgcg tgacgcgatg ttcaggcagc tcgccgctgc gctacagctt ccagcactga
    10621 ctgtcgcgca ggcatttgcc gtgaaccgct acttcgaagc gctcgagctt cgcgagtacg
    10681 atcagctgag cgagatcgtt accgacgatg tcgtgtatca actgcccggc acggacgcgc
    10741 gcttctctca ggagatccgt ggccgcgacg cgttcctcga attcacgaac aagacgttca
    10801 acggattcga tcgtgcgcac ttcgaggtca acgcgattcg gtcgcttccg aacggcgcga
    10861 tggtcgaata cgtaggtcat tggaaggaag cgaaccggga acgcgcgctc cccggtgccg
    10921 tcatgttcga gctccgcgac gaccgcatct ctcgaatcgc ggtccgcgtg aaccctgacc
    10981 ggctcgtcta gcggcaagcg cagttgcagt agccggaccc gccgcgcgta gacccgcgac
    11041 atcgctcggc cccgggcagg cctgaccctt gcttgacgca aggcaccatc cttcgtttgc
    11101 cttgtggagc tgccgtcgtg aaaacctggt ccgtttgcct gctggtctcc ctgtgtggcg
    11161 cgagcgcctg catcggttcg gaagacgacg agaacatcga cgtgacggtc ggcgacgaga
    11221 aggccgatgc ggtctcgcat gtcacggtca acgccggatc gacctcgacc gttaacttca
    11281 ccgcgactgc gggtgcggtc gacatcaccg tcgattgcgc tccgcccgcg aatcccgaca
    11341 cggtcggcac gcagttcacc gtgaaaggta cggggctcgc tggcctatcg agcgcgagcg
    11401 cgccgatcgc cggcctctgg caatggagcg gcaccgtcac cgcggggacg aagaagctcg
    11461 agctcaaggg cgtgtccggc tcgcgcagct gcaccgttcg cgtgcgcaag gcgacgggtc
    11521 agtgcacgtc gtcgaccgta acgcgctcgc ccgagacggg gcacacgcat ctgttcgtca
    11581 attcgaacct gaccacggcg ccattcccag catcgggcaa ccactggggc gcgtgggcga
    11641 agtggggaac gatctactcc aagccggtca agcgcggctt ctacttgcat aacctcgagc
    11701 acggcggtct cgtgctctcg tacaagtgca cctcgtcgac ggcgagcgcg gagtgcgccg
    11761 atgccgcagc gaagatgacg gcgctcgcga attcgttcgg cgagtaccgc gtgatcgtga
    11821 cgccggaccc gcagcagccc acgctgttcg gcattcgcgg ctggcgcgtt ggctatcagt
    11881 cggactgctt caacgagcaa cgcatgctcg acttcatggg cgatcacttc cgcaatggac
    11941 gcgaagacac cgatgcggat cctccggtgc cctacgatcc gacgacgtcg caggttccgt
    12001 gcgtcgacct gatggcggcg cccgatagct gcaactagac tcgcagcatg cgagttgccg
    12061 ttctcggcca cgtcgaatgg gtcgagttcc tccgcgtgcc agccgtcccg catgcgggcg
    12121 acatcgtcga gggcgttccg ctcgtcgcgg tgcctgccgg cggcggtggt gtcgccgcgg
    12181 tccagctcgc tcgctgggcg cgctcgtgcg acttgttcac ggctctcggc gacgacgagc
    12241 tcggccatcg ctgtgaagca cagctgcggg cgcgcggtgt gaacgttcac gctgcgtggc
    12301 gcgccgagcc gcagcgccgt gcggtgacgc tgatcgacgc gcagcgcgag cgcaccatca
    12361 tcctcagtgg ccatcgccac atcgcgaagc gcgccgatgc cctgccgtgg gacgcattcg
    12421 gcgacatcga taccgtctac gtcaccggcg gtgacgccga tgctgtgcga gcggcgcgcg
    12481 cggccaaggt cgtcgtcagc accgcgcgtg ttctgccgct actgcgcgat gcggcgatca
    12541 cgctcgacgc gctcgtcggc agcgataacg atcccgccga aatctatcca ggcgatttgc
    12601 atccttcgcc tcgtctcgtc gtgcgaacga acggcgcgat gggcggcatg tacacgctcg
    12661 ccggcggagc gccgcaggcc tacgctgcga tccagacgac tgtcgtgggc gacacgtacg
    12721 gcgcgggcga tacgttcgcc gccgcgatcg cgttcgggct cggcgagggg cggcagccag
    12781 gtgacgcgat cgcctttgcc gctgcacgtg cggccgaggt gctggcgttc catggtccct
    12841 acggttgatg atgctgcact gatgtcgacc gagcccgaac agttaccgat gggcacgaag
    12901 atcgcgacca tggtgggcgc gctcctcggc ctctttctcg ccgcgctcga tcaaacgatc
    12961 gtatcgaccg ccggtccgtt catccagaag gacctgtcga tcgaaccggg catctatgtc
    13021 tggatcacga cggcgtacct cgtggcctcg acggtgctcg tcccgatcta cggcaagctc
    13081 tccgacctct acggccgccg ccgcatcttg ctcgtcgcga tcggcatctt cctcgcgggc
    13141 tcgctcgcat gcggtgcatc gcaaaccgct gcgcagctca tcgcatcgcg cgccgtgcaa
    13201 ggcgtcggct ccgcgggcct gttcacgagc gcttttgcga tcgcggccga cctctgggct
    13261 cccgccgagc gcggcaagtg gcagggcatc ttcggcgccg cgtggggcgt gtcatcggtg
    13321 atcggcccgc tcctcggcgg cgtcctcacc gagagcctca gctggcactg gacgttcttc
    13381 gtgaaccttc cgatcggcgc ggtcgctgtc tcgctgatca tgtccaagat gccgccgctg
    13441 cggcgcgagc agacgacaaa gccaaccctc gacatcgccg gcgccgtcgc gcttgcggtc
    13501 ggcacgcttc ccttgcttct cggactctcg ctcggtcacg ccgaggggac cgggcaaacg
    13561 acgggctggc cgtggaagtc ctggcagatc gcagcgctgt tcgcgacgag cgcggccggc
    13621 atcatcgcct tcattctcat cgagctgcgt gcgaagaatc caatcctcga cttcaagctg
    13681 ttccgcatca agacgttcgc gatcggctcg ctcgccacgt tcgtcgtcgg cagcgcgttc
    13741 ctcggcgcga tcgtgttctt gccgctgttc atggtgaacg tcgtcggcct ctccgccacg
    13801 agctcgggtc tcacgaccat gccgctcacg ttcggcatcg tcgtcgcgaa catcgcgtcg
    13861 ggacagctcg tctcgcgcct cggcgcgtac aaaggcctgc tgctcgcctc gcttgtcgtc
    13921 ctgtgcgcga gcttcctgtg gttcgcgctc acgctcgaca tcgacgcgac acgcgcgagc
    13981 atctcgatga agatggtctt cctcggcatc ggcctgggcc cgtcgatccc gctgttcacg
    14041 ctggcgatcc agacgtcggt gccaatgcag cagatcggcg tggcgaccgc ctcggcgacg
    14101 ttcttccgcc agatgggcac cacgatcggc atcgcgatca tcgggacgat cttcgcgacg
    14161 tcgttcgccg gcgcgctcaa tcgcgagatg ccgaagcccg aggagatgcc accgcagatg
    14221 cgcgcgatga tggagcgcaa aggtggtggc ggtggcgaag gcggcgcacg cgtcgcgttc
    14281 gatgccaagg ccgcaagggc aagagcggat gccggtatcg attcatcgcc gatgcccgac
    14341 ttcgcgaagg cgcaagcgaa agagaacgcg cacgtcgcga tcagcaaggt cgatcatgcg
    14401 ttcaagcgcg gcttcaccaa tgcactgatc aacgtcttct gggtgtcgct cgcgatcgcg
    14461 atcgccggcc tgctcatcac cgtgttcttg ccgcaactgc cgctgcgcaa aggcttcggc
    14521 gctccgccac cacccgccga atagctaacg cacgacggtg aacacgaagt cgcgtgatgc
    14581 ccccgcgtga cacccgatgc agctctcgac gtcctggccg atggacgaga cgtcgtcgcc
    14641 gatgccttcg taccagtacc agctcgcgcc gccgggcacg tcatcgagcc ggcgggaggt
    14701 cgcgtacacc acgatgctcg cgccgcgaaa tacttccttc accgacgccg cacctgcggg
    14761 ccagtctccg ccgtcctcga tcgcctcgta gagcttcgca gtgccagtcc ttgtagtagc
    14821 cggcctcgac ccacgtcgcg atgtcgcgag agccgcgcgg cggcagctgg cgatccgcga
    14881 gatcgtcgcc gagcgactcc ggtggatcgg tgcacgcggc gagaccaagg accaggatgg
    14941 cgatgcgcat agggtgccta cgcctggacc gaacgcgggt tacgcccgat gtccggaatc
    15001 gtgtcctggt ccgatcgggc ctggcggcgt acgtgtgagg catgagtacc ggaatcgaag
    15061 tgccgtggtc gcgcttgctc ggcatcgcgg gtgccttgct cgcactgcga acgcaggcat
    15121 gcgagccgac ccgaacgatg ccagcaacga tcgccaccga cggcaacgcg ctcctggtcg
    15181 cgtgggagaa accgacggac gacggcttca cgatctacat gcgccacgtg cccgacgtgc
    15241 aggacggcac gacgacgctg cagggcggga cggctatcta cacgaagaag tacggcacga
    15301 cgggctatca cgggcccgag gtcgcgaccg ggcccgcggg gtcgatcgtg atcatcacgc
    15361 cccgagtcgg agagcagatc gcgattccgg tcgatctcga cggacgcgtc gcgggccccg
    15421 cgcagccact caccggatgt cggtcggaca gctcggatcg cacgtgtttc tggtcatgcc
    15481 ggcgaccgat cgcacatgca ggcggattca tcgtcgggca cgtgtctgcg tatccgcgaa
    15541 gctcgatcca cagcctcgat ctatcgttcc tcgatcgcgc aggacggacc gagaaattcc
    15601 tcgtgctgcg ttcgcaagat cctgtcgcct gtgccatggc cgcgagcggt gacgagctcg
    15661 ttgtcgtttc caccgagcgc gaaacgctcg ttgacgtaaa gattcacgtg ctgttcttgg
    15721 cgctctccga tggtgagctg ctcaacgagc tctggatcga cggcgggtac gcattcggca
    15781 tcgtatcgac gagcgccaac gagtacgcgt tgcttcacca gatccccgat ggccgcagcg
    15841 tgctgaccaa gttcaaccgc aagggcatcg agtcgacgca cttagtcccc gaggcaatcg
    15901 atctgcgaac ggcggatctc gggatgtcgt ttcgcgggct gttcgtgagc tggctcgcga
    15961 acggccgcgc tcacgtcctc gatctcacga cggaccgcga ggtgagccgc aagggtgtcg
    16021 cgcgttactc cgtcggaaca cgtgcggtcg gggttcgtga tcgatgcgtg accgcgtgga
    16081 cgagcggagc cggcaacaag gtgcagctcc tcgccgttcc cgagtgccca tgatcaggac
    16141 gcgcggcgtg gcgtgcagag attcacgcgg cgtaacagat tctcgtcttc cagcgcggct
    16201 gccatgctac gcgcacgtgc gttgacggct tgtgcctgtt cgtttggcac gacgcggctc
    16261 aacatattga gagcttggat ccgcatccgc acgaggccgc catcaccggc gtcgtcggca
    16321 gcgcgctcgg cgagccggcg agcttcgtcg tcgttgccct gtcgccacgc gcagtacgcg
    16381 aggcccgcgt acttccgaaa cgacgcgaac ggcacgctcg actcgggaat cgtttcgtaa
    16441 tgcgcacgtg cgcgatcaaa atcggccggg tctcccgcgg cgggccgcga gaaatggcgc
    16501 gag
//
LOCUS       DATLBZ010000617        15505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10952979, whole genome shotgun sequence.
ACCESSION   DATLBZ010000617 DATLBZ010000000
VERSION     DATLBZ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15505
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10952979"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VMZ53_30345"
     CDS             complement(<1..249)
                     /locus_tag="VMZ53_30345"
                     /inference="COORDINATES: protein motif:HMM:NF024402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32855.1"
                     /translation="MSDSAITRDTAGRFVVLGTLYFAQGLPFGFFVQAIPVILRQGGV
                     SLSKIGFTSLLTLPWALKFLWAPYLDRVYSPRLGRRRTW"
     gene            395..3460
                     /locus_tag="VMZ53_30350"
     CDS             395..3460
                     /locus_tag="VMZ53_30350"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32856.1"
                     /translation="MARVLANRFELGERVGKGSLSVVHDAIDRETGRRVAIKLAAGRE
                     DKDSVRQRFDREAAALSVIQSAHVVELIWAATDEDGMPFLVLEKLIGKNLQEVIGERG
                     PFKPERVGRYMAQAATALELAHGLGIVHRDLKPANLFLHDTGEDRRILKVLDFGLVVD
                     TGGPTERMRDAFGGTPLYMAPEQARGQLTRIGPATDIYAIAHVTLTLLTGETYWTDGL
                     AEDVMREIEAGVSTKPSQRWPWLTDAFDQWFMRSTQRVPERRYKSVVEQAARLVEALR
                     GLKAPDQRTPAPSLVAASTIAARTPTPSIVRLAGHRTPVIGRGIEYRDIDQLLARGKV
                     VTLTGAAGVGKTRLAQAICETAGERFIDGTWFVRLVAGSSGDDGDGAKPVLGAIASTL
                     SIEPDATRSLFDNVVDNLAMRRVLIVLDGVDRVAGSSAIIDELVRRCPAVTWLATSHA
                     PLGLASEHKYVVEALDVPPAENVALDEAETYAGVALFVACAREVTPMFAITDDNVADI
                     VAICRAVGGSPLAIEIAAAQVSSAKPAQIRAGLERGGAHAGSTVRHAIMSSYALLSPD
                     QQRVLRQLAILPAGLTFEQVKLELAHLSDDPMYAVLRLVQTHLATWSSDTPRRLRMLD
                     TVREIARDLSRQAGEDASLWQFARKHAYAIATSSSQSTTDAWLARVDLEYDNLRAVLG
                     HLLHNAPADAMELAGRLAFYWYLRGSYADGMQWLEAAIARSTGADDERESLARALLGA
                     GRLALLTCHYARAQELLSQARSLSIALGDLPSEANADQLLGSVARELGEYTQAHNFHA
                     RSLDIWQRINNAHEIARARNYLAFAAWIGDYAGMPSDELLAWWNDIDEQALRSLADPE
                     ITVWYLLNRGAILHHKGDPAARDILGQAFAEAVAARFYEGIAWSLDQIGRVSLERGHL
                     LQARAQLSASLRVHRRLGDRWRCASVLEALASASVASDRPARGAVYLGAADTIREQIN
                     APVPACERPMLASTEARGVDMIGQAFDAGRERGHRTTLDQTIELSRDVV"
     gene            3694..4932
                     /locus_tag="VMZ53_30355"
     CDS             3694..4932
                     /locus_tag="VMZ53_30355"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS32857.1"
                     /translation="MRSPWWQGLDLRVLRHVRGVLRRRGLELTLVEKRPSEPAAFASL
                     SVDDPGGPGLAVIAPDLSSPEVGLIEHMLGEAALDVAAALRERDGQGDPNGRRASYAG
                     IIGSAPSMLELYSLLDRVAPSDSTVLVQGENGTGKELVARAIHDRSMRREHRFVVTNC
                     SAFNDNLLDSELFGHKRGAFTGAVADKAGLFETADLGTFFLDEIGDMSPTLQVKVLRV
                     LQEGTFNRVGDTETRKVDVRIIAATNRDLRAMVANGQFREDLFYRVNVINLTLPSLRE
                     RRDDVPVLIDHFLARHRRNGRPKKLTADCQAQMLAYPWPGNVRELENEIERLVVLAGD
                     SATIDTSLLSPRIRQYAPTEEEAPVDAASLPAAVEALERKMIGAAIRKYGGNKTRAAE
                     ELKVSRRNLIRLVQKYQLEK"
     gene            5028..5804
                     /locus_tag="VMZ53_30360"
     CDS             5028..5804
                     /locus_tag="VMZ53_30360"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HUS32858.1"
                     /translation="MFRGVDLRFVGITAIAIAMVCSTWLLTDTYHSIKVKPEQRTIRV
                     TGSAKKRIVSDLIEWTAVLEARAPDRTAAYKTLRVDTDKTIDFLEKQGIKADEIQPQS
                     ATFEEDIEVTQEVKMMPGALSPVTVEKKTFKGYITRQSILVRSKDVQLIEKASREVTS
                     LLEDGISITSDPPEYFYTRLGELKVEMLAAAGKDARARADNILKSTGGAGISRLLDAD
                     MGIININPANSTETSEEGNNDRTSLEKDIITIVHADFELN"
     gene            complement(5811..6737)
                     /locus_tag="VMZ53_30365"
     CDS             complement(5811..6737)
                     /locus_tag="VMZ53_30365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HUS32859.1"
                     /translation="MKIVVTGATGFIGRALVSELVEQGHHVVAFTRGKRLERVENVEV
                     QTLESQGPWCDALAGADAIAHLAGAPVNGTKWDARGKQLIRDSRVESTRTIVEALTKL
                     TPASARPHTLITASGVDYYPFAYDETDFDDDEVTESDKPGDTFLARVCRDWEREALNA
                     AAAPLNLRVACMRTGLVLGKGGGALPELARPFKFFAGGPVGDGRQWVSWIHLDDVVAA
                     YIAALTDDRYRGPINMVTASIRNRDFASALGHAMSRPAFLPVPKFAVKLVAGKEFAES
                     ILKGRRVMPTKLRELGFAWKYPTLERALAAAV"
     gene            complement(6734..7507)
                     /gene="thiD"
                     /locus_tag="VMZ53_30370"
     CDS             complement(6734..7507)
                     /gene="thiD"
                     /locus_tag="VMZ53_30370"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HUS32860.1"
                     /translation="MPDVLVCAGLDPSGGAGLVADVRVVSELGARPCGVVTALTIQNT
                     TGVVGSHPCDPDVVGHQLAFLMTDVEMKAIKIGMVGSAAIARAIANALHLSAAPVVWD
                     PVMFASRGNAPLTDDIEEVMLALRPHLTLVTPNKQELFLMTGLPTGTPVMAEAAARTL
                     ARKLDTAVLIKGGHLGGDESVDVLIHAGNPGPMIELRSPRILGGEDVHGTGCALSSAI
                     ATYLANGRELDEAVQLSKQYVTRLIENPVHPGRGAAAVR"
     gene            complement(7542..8708)
                     /locus_tag="VMZ53_30375"
     CDS             complement(7542..8708)
                     /locus_tag="VMZ53_30375"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS32861.1"
                     /translation="MTYAALDYYALDELFTHEERMVRDTVRELVSSRVMPSIGKHFSA
                     GTFPHELVSVFGEMGLLGPSLTGYGCAGISPTAYGLICQELERGDSGIRSFCSVQSSL
                     VMYPIWAFGSEEQKQKWLPEMAAGRVIGCFGLTEPDFGSNPNGMLTTATREGGGYRLN
                     GTKRWITNGSVAHLALVWAKLDGVVRGFLVPTKTKGFTARDIHGKWSLRASVTSELIM
                     EDCVVGEEALLTGVSGMRGPLSCLSQARFGICYGVVGAAMACYDEALSYAKDRVQFSR
                     PIAGYQLVQRKLVDMVQEITKAQLLCWRLGRLKESNKLTPAQISLAKRNCVAMARDCA
                     RDARDILGANGVTDEYQCGRHMLNLESVYTYEGTHDIHTLVVGQDVTGLAAFDG"
     gene            8755..10824
                     /locus_tag="VMZ53_30380"
     CDS             8755..10824
                     /locus_tag="VMZ53_30380"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HUS32862.1"
                     /translation="MRVLVLLVLLASSVARADAPNAKKLGEAYDAYGASDLAKAKKLL
                     GSLDDTKLVATDYALWLRGMVALRGGDPATAQKAFEKLAKMKGTSFAAEVPWRLADCA
                     WEKGDKTGAAKAYAKLIKSGNASDTGDIGTAKFRIAEANPSAAAYRAVVIDHPAHPLA
                     AKAEARRLELGGSPLTSEDRIERAKQLNAAHLWDQAIVELALVPQTGVSDSLAAQRDY
                     WTGNTLFEMRRRYADAATLLLGVYEKLNSAEAMFHGARALSRADKDDEAIVWYGKVVD
                     KYPRTSWAEEAAFLRGWLEFNRGHYKEAIKPLEESLAKYPKSKWVDDGHWFLGMSHYF
                     LGEWDAARTELGKLAKFSGALEGGKGTYWLARIDEKLKKNDSAIAGYKDTVSDHPFSW
                     YALLARARLGQLGVKIGPFGVENPQPKGGKLSATVDEKLATDELIKKVDELITADMGV
                     DAGNELVRGEKAFLKRHDRGSAFAMLLDRYRKAGNFNRPWLLAVIYGGSALDGPPEGD
                     ARRWWENAYPRAYRDLVEKYQDLGKNPEGYLYSIMRKESGFNPHDVSYADAQGLLQMI
                     PPTTMRVAKVLGIPYDPGKLYEPEYNIHMGGWYIGHLLQKFKGQIPFGAGSFNSGPRP
                     VMKWLDLYGDRDVDEAVELVPYTQTREYMKKVTENYARYRYLYNNEVYDQPLTVDKAY
                     LKDQLTY"
     gene            complement(10838..11485)
                     /locus_tag="VMZ53_30385"
     CDS             complement(10838..11485)
                     /locus_tag="VMZ53_30385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32863.1"
                     /translation="MKLAIVIVLALTSSAAARVVMTPTVPRTCGTAESWDKVMKCLER
                     FGTPKLERSVDGAKLVGIAYPKFNVPGLYLYRSVGKRWVIAGMFEAIGTFDVRGFSRP
                     TIAKHTGYRFDVGVVQSETSDETSSANEVRRKVSVFCDGGTYRCTEVTTSCERLLDGR
                     ATETFRGKLAIKGDIAKVTGDRSRAGTMCDVPDELQLDFAQVNRIPAMPIGDPLF"
     gene            complement(11482..12201)
                     /locus_tag="VMZ53_30390"
     CDS             complement(11482..12201)
                     /locus_tag="VMZ53_30390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32864.1"
                     /translation="MRVVCAVLASILMSGVAFAHPVRDPFDEPFDRDDLIRRKGSKRK
                     LQPVRTPEELCERNKSWPKLVKCLAQGGNTVATMRDLGKGRLVSVKSNPSYDSTSVFI
                     YTKQDGRWQRMSGYFATNATSEILSFERLKKENGYRLEQGQIFKSTAFSTERFPGADQ
                     TRTPVTMRRKTTSICLDNGYCQTFITSCDALVDGKTRWSFRGTLLIDHGVVRMTGDRA
                     YAGQVCAPAMSQLSNVGDILE"
     gene            12256..12468
                     /locus_tag="VMZ53_30395"
     CDS             12256..12468
                     /locus_tag="VMZ53_30395"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HUS32865.1"
                     /translation="MKLKGVLTHSDLEGGHWLLKTDSGDEYQLTGAIKDCKDGQRVEV
                     EGKVDKQAMGIGMMGPHFNVSKLTAL"
     gene            12481..12747
                     /locus_tag="VMZ53_30400"
     CDS             12481..12747
                     /locus_tag="VMZ53_30400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32866.1"
                     /translation="MGLAITDFATSTGALRDIYDRMRERPLPPAYRPAHGGVPGIITA
                     HSLDPQLIGKVFSTSTTLNGAGPLTWPERELVNATTSRLNQCFY"
     gene            12811..13491
                     /locus_tag="VMZ53_30405"
     CDS             12811..13491
                     /locus_tag="VMZ53_30405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32867.1"
                     /translation="MIADARHVAQHAPNARMKMIAELTTIVTEAPWTLSRSVLGRAHS
                     AGLSDEDVLHVVLLSSYFGHLNRIADATGVPLDYNVKLEVPATDPACPPWPAAPSLIA
                     GRHAIDIARRAATATALSDWRTYIFYRDAPLTRRQRTLIARWVATWLGDGGISPPTDL
                     TVNPIDDHLRAVAEVVTLAPWKITDATFAPLRESGFDDAGIFDVCATATSAGVFSRIE
                     VALVALGH"
     gene            complement(13787..>15505)
                     /locus_tag="VMZ53_30410"
     CDS             complement(13787..>15505)
                     /locus_tag="VMZ53_30410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32868.1"
                     /translation="LAQGVVAGGAKKPAPSGADVELGAPGARGVEVQADAPNAPDLEL
                     GAPGARTGAKGAAAAKGADIALGASGRSADVSNVSVDLADHVDIGAADGKEAVRASQV
                     MKAVDGPPELAAELESKPAAASKSAAAEKPAAKAAEKPAAKVEPKAAEKPAAKAAEKP
                     AAKAAEKPAAASKSPAVEKRPSVAPVAPRQGVSPVLIAALVIAILAAGGFALYKFVLK
                     KKDTTESTTNTPVTTPKQPEPPPPPPVESQKLAVEQPAPEDLKPTDAHQLAWIAANDT
                     VVKAGDPVAKYVGFKSLEADVKNITTALDKAKADVAAAGSDEKKLAAAQKAQADNEKK
                     LADKQGALDKFLLKAPAAGKVTAVAKINAKVTPTEVVATLQRDAILVATFKKADGATD
                     NAPVLLAVKGSDQKLACNVVLASGDGTKIACPSGAAAEGTEVTFAGLDSSRPATGETP
                     TETPPTGDTGSAGSAAGTGSGAAGGATPETGSGAGTGSGGPNTGSNGEGKGAGEEKAA
                     KPPTPKATRPVRRPVQRPAGGQGDKGTGSAATPAGGDKPAGGETTPTPAPTPAPPPAP
                     AGSSSL"
BASE COUNT         2724 a         5159 c         5036 g         2586 t
ORIGIN      
        1 ccacgtgcga cgccgcccga ggcgcggtga atacacgcgg tcgagatacg gtgcccatag
       61 aaacttcagc gcccacggca gcgtgagcag agacgtgaag ccgatcttcg acagcgagac
      121 gccgccctgt cgcaagatga cgggaatcgc ctgcacgaag aagccgaacg gcaggccctg
      181 cgcgaagtag agcgtgccga gcacgacgaa ccgcccagca gtatcacgcg tgatcgcgct
      241 atcgctcatg cgtcagccat tcgatgctcg agtcggagaa cgcgacgcgc acgtcgatcg
      301 cgcatccgat gatcgctcga aacggcgaat gaacgcgagg gcagatctgc gggatgcgcg
      361 gcgatgtacc cgccctcgcg ataagctcgg ggcgttggcg cgcgtcctcg caaatcgctt
      421 cgagctcggt gagcgcgtgg gcaagggcag cctcagcgtg gttcacgatg cgatcgatcg
      481 cgagaccggg cgtcgcgtcg cgatcaagct ggcggcaggt cgcgaggaca aagactcggt
      541 gcggcagcgc ttcgatcgcg aggcggccgc gctgtcggtg atccagagcg cgcacgtcgt
      601 cgagctgatc tgggccgcta ccgacgagga cggcatgccg ttcttggtgc tcgagaagct
      661 catcggcaag aacttgcagg aagtgatcgg cgagcgcggc ccgttcaagc cggagcgcgt
      721 cggccgctac atggcgcagg ccgcgacggc gctcgagctc gcgcacgggc tcggcatcgt
      781 ccaccgcgac ctcaagccgg cgaacttgtt tctgcacgac acgggcgagg accggcggat
      841 cctgaaggtg ctggacttcg ggctcgtcgt cgatacgggc gggccgacgg agcgaatgcg
      901 cgacgcgttc ggcggtacgc cgctctacat ggcgcccgaa caagcacgcg gccagctcac
      961 gcgcatcggc ccagcgacgg acatctacgc gatcgcgcac gtgacgctga cgctgctgac
     1021 gggcgagacg tattggaccg atggcctcgc cgaagacgtg atgcgcgaga tcgaagcggg
     1081 tgtgtcgacg aagccatcgc agcgctggcc atggctgacc gatgcgttcg atcagtggtt
     1141 catgcgcagc acgcagcgag tccccgagcg acgctacaag agcgtcgtcg aacaagcggc
     1201 gcggctcgtc gaggcgttgc gtgggctgaa agcgccggat cagcgcactc cagccccttc
     1261 gctcgtcgct gcgagcacga tcgctgcgcg cacgccgacg ccgagcatcg tgcggctcgc
     1321 gggccatcgc acgccggtga tcggccgcgg gatcgaatat cgcgacatcg accagctcct
     1381 cgcgagaggc aaggtcgtga cgctgaccgg cgccgcaggt gtcggcaaga cgcgactcgc
     1441 gcaagcaatc tgcgagaccg ccggcgagag gttcatcgac ggcacgtggt tcgtgcgcct
     1501 cgtcgcaggc agctcaggcg acgacggcga cggtgccaag cctgtcctcg gcgcgatcgc
     1561 aagcacgctg tcgatcgagc ctgacgcgac gcgctcgctg ttcgacaatg tcgtcgacaa
     1621 cctcgcgatg cgtcgcgtgc tgatcgtgct cgatggtgtc gaccgagtcg ccggcagctc
     1681 ggccatcatc gacgagctcg tgcgccgctg ccctgccgtg acatggcttg ctaccagcca
     1741 cgcgccgctc ggcctcgcgt cggagcacaa gtacgtcgtc gaagcgctcg atgtcccgcc
     1801 ggccgaaaac gtggcgctcg acgaagccga aacgtatgcc ggcgtcgcgc tgttcgtcgc
     1861 ttgtgcccgc gaggtgacgc cgatgttcgc gatcacggac gacaacgtcg cggacatcgt
     1921 cgcgatctgt cgtgcggtcg gcggctcccc gctcgcgatc gagatcgccg cggcgcaagt
     1981 cagctcggcc aagcccgccc agattcgcgc aggactcgag cgcggtggcg cgcacgcggg
     2041 ctcgacggtg cggcacgcga tcatgtcgag ctacgcgctg ctctctcccg atcaacagcg
     2101 tgtgctccgc cagctcgcga tcctcccggc cggcctgacg ttcgagcagg tcaagctcga
     2161 gctcgcgcac ctcagcgacg atccgatgta cgccgtcctt cgcctcgtac aaacgcacct
     2221 cgcaacgtgg tctagcgaca cgccgcgccg ccttcgcatg ctcgacaccg tccgcgagat
     2281 cgcgcgcgac ctctcgcgcc aagccggcga agacgcctcc ctctggcagt tcgcacggaa
     2341 gcacgcctac gcgatcgcga cgagctcttc gcagagcacg accgacgcat ggctcgcgcg
     2401 agtcgacctc gaatacgaca acctgcgcgc ggtcctcggt cacctcctcc acaacgcgcc
     2461 cgccgacgcg atggagctcg cagggcgcct cgccttctac tggtacctcc gcggcagcta
     2521 cgccgacggc atgcagtggc tcgaagccgc aatcgccaga tccacgggcg ccgacgacga
     2581 acgcgaatcc ctcgcgcgcg cgctcctcgg cgccggtcgc ctcgccctgc tcacctgcca
     2641 ctacgcccgc gcgcaagagc tcctctccca agcccgctcc ctctccatcg ccctcggcga
     2701 cctgccaagc gaagccaacg cggaccaact cctcggcagc gtcgcgcgcg agctcggcga
     2761 atacacgcaa gctcacaact tccacgcacg cagcctcgac atctggcagc gcatcaacaa
     2821 cgcccacgaa atcgcgcgcg cccgcaacta cctcgcattc gccgcatgga ttggcgacta
     2881 cgcaggaatg ccaagtgacg agctcctcgc ttggtggaac gacatcgacg agcaagccct
     2941 ccgctccctc gccgatcccg aaatcactgt ctggtacctc ctcaaccgcg gcgcgatcct
     3001 ccaccacaaa ggcgaccccg ccgcccgcga tatcctcggc caagcgttcg ccgaagccgt
     3061 cgccgcgcgc ttctacgaag gcatcgcgtg gtcgctcgac caaatcggtc gcgtctccct
     3121 cgaacgcggc catctcctcc aagcgcgcgc ccaactctcc gcctccctgc gcgtccaccg
     3181 ccgcctcggc gatcgctggc gctgcgcctc cgtgctcgaa gccctggcct ccgcgtccgt
     3241 cgcgagtgat cgccccgcgc gcggcgccgt ctacctcggt gcggccgaca cgatccgcga
     3301 gcaaatcaac gcgccggtcc ccgcatgcga acgcccgatg ctcgcttcca ccgaagctcg
     3361 cggtgtcgac atgatcggcc aagcgttcga cgccggtcgc gaacgcggcc accgcacaac
     3421 cctcgatcaa acgatcgagc tgtcgcgcga cgtcgtctaa cccgcaacca aactggtctt
     3481 accgctaagg gacggacgca acttccgcaa ccgccgcaac cgcgagggac ggagcagtgg
     3541 cctgaccgct aagggacgga cgcaacttcc gcaaccgccg caaccacgag ggacggagca
     3601 cgtccgtcgc gcggacaccg cagtcgcgtt agcgacggat cgcggtgggc tcttgcgatt
     3661 ggtgtgacga gttgtcacac tgcggcttcg ctaatgcggt ctccttggtg gcaggggctc
     3721 gatctgcgcg tgcttcgaca cgtgcggggc gtcctgcgtc gtcggggcct cgagctgacg
     3781 ctcgtcgaga aacggccgag cgagcccgct gcgttcgcgt cgttgtccgt cgacgatcca
     3841 ggtggtccag ggctcgcagt gatcgcgccg gatctctcgt cgccggaagt agggctcatc
     3901 gagcacatgc tcggcgaagc agccctcgac gtggcggccg ctctgcgcga gcgcgatgga
     3961 caaggtgatc cgaatggccg gcgcgcgagc tatgcgggaa tcatcggcag cgcgccgtcg
     4021 atgctggagc tctactcgct gctcgatcgc gtggcgccca gcgattcgac ggtgctcgtt
     4081 cagggtgaga acgggacggg caaggagctc gtcgcgcgtg cgatccatga tcgctcgatg
     4141 cgtcgggagc atcgattcgt cgtcacgaac tgctcggcgt tcaacgacaa tctcctcgac
     4201 tcggagctgt tcggccacaa gcgcggcgca ttcacgggcg ctgttgccga caaagcgggc
     4261 ctgttcgaga ccgcggacct cggcacgttt ttcctcgacg agatcggcga catgtcgccg
     4321 acgctccagg tcaaagtcct gcgcgttctg caggaaggca cgttcaaccg cgtcggcgac
     4381 acagagacgc gcaaggtcga cgttcgcatc atcgccgcca ccaaccgcga tctgcgagcg
     4441 atggtggcaa acggccagtt tcgcgaagac ctcttctacc gcgtcaacgt catcaacttg
     4501 acgctccctt cgctacgcga gcgacgcgac gacgttccgg tattgatcga tcacttcctc
     4561 gcgcgacacc gtcgcaatgg cagacccaag aagctgactg ccgactgcca agcgcaaatg
     4621 ctcgcgtatc cgtggcctgg caacgtgcgc gagctcgaga acgagatcga gcgcctcgtc
     4681 gtgctcgctg gcgactccgc aacgatcgac acctcgctgc tgtcgccgcg gatccgtcaa
     4741 tacgcgccaa ccgaggagga agcgcccgta gatgcagcgt cgctgcccgc ggccgtcgaa
     4801 gcgctagaac gcaagatgat cggcgccgcg atccgcaaat atggcggcaa caaaacgcga
     4861 gccgcagaag agctgaaggt ctcgcgccga aacctgatcc ggctcgtcca aaagtatcag
     4921 ctcgaaaaat aacccgcgaa acctgccgcg aaacgaaata gctcccgtgc gtggcgcgtt
     4981 cgcgcgggat ggaacgaatt gcccctgact ccccatcccg cgccggcatg ttccgcggcg
     5041 ttgaccttcg gtttgtcggc atcaccgcga tcgcgatcgc aatggtctgc tcgacgtggc
     5101 tccttaccga cacgtaccac tccatcaagg tgaaaccgga gcaacgcacc attcgcgtca
     5161 ccggctcggc gaagaagcgc atcgtctcgg atttgatcga gtggacagcc gtactcgagg
     5221 cgcgcgcgcc agaccgcacc gccgcctaca agacactgcg cgtcgatacg gacaagacga
     5281 tcgacttcct cgagaagcag ggcatcaaag cagacgagat ccagccgcag tccgccacat
     5341 tcgaagaaga catcgaggtg acgcaggaag tgaagatgat gcccggcgcg ctctcgccgg
     5401 tgaccgtgga gaagaagacg ttcaagggct acatcacgcg gcagtcgatc ctcgttcgca
     5461 gcaaggacgt gcagctcatc gagaaggcgt cgcgcgaagt cacttccctg ctcgaggacg
     5521 gcatctcgat tacgtcggat ccgccggagt acttctacac tcgcctcggc gagctcaaag
     5581 tcgagatgct tgctgcagcg ggcaaggacg cacgcgcacg cgcggacaac attctcaagt
     5641 cgacgggtgg agccggcatc tcgcgcctcc tcgacgcgga catgggaatc atcaacatca
     5701 accccgcgaa ctcgacggag acgtccgaag aggggaacaa cgaccggact tctctcgaga
     5761 aggacatcat cacgatcgtc catgccgact tcgagctgaa ctgaaaggga tcagaccgcg
     5821 gcggctagcg cgcgctcgag ggtggggtac ttccaggcga agccgagctc gcggagcttt
     5881 gttggcatta cgcggcgacc tttgaggatc gattcggcga actctttgcc cgcgacgagc
     5941 ttcaccgcga atttcgggac cgggaggaat gccgggcggg acatcgcgtg gccgagcgcc
     6001 gaggcgaagt cgcggttgcg gatcgaggcg gtcaccatgt tgatcgggcc gcgatagcga
     6061 tcgtcggtga gggctgcgat gtatgcggcg acgacatcgt cgaggtggat ccacgagacc
     6121 cattggcgac cgtcgccgac agggccacct gcgaagaact tgaaggggcg ggcgagctcc
     6181 ggaagtgcgc caccgccttt tccgaggacg agaccggtcc gcatgcatgc gacgcggaga
     6241 tttaggggag cggcggcggc gttcagggct tcgcgctccc agtcgcgaca gacgcgcgcg
     6301 aggaacgtgt cgccgggttt gtcggattcg gtgacttcgt cgtcgtcgaa gtccgtctcg
     6361 tcgtaggcga atgggtagta gtcgacgcca gaggcggtga tgagcgtgtg cgggcgggcg
     6421 gaggccgggg tgagcttggt gagggcttcg acgatcgtgc gcgttgattc gacgcgcgag
     6481 tcgcggatga gttgtttgcc gcgcgcgtcc cacttcgtgc cgttgaccgg ggcgccggcg
     6541 aggtgcgcga tcgcgtcggc gccggcgagg gcgtcgcacc aagggccttg gctttcgagc
     6601 gtctggactt cgacgttctc gacgcgctcg agtcgcttac cgcgcgtgaa cgcgacgacg
     6661 tggtggcctt gctcgacgag ctccgagacg agcgcgcgac cgatgaaacc ggtggcaccg
     6721 gtgacgacga tcttcatcgc acggctgccg caccacgacc tgggtgcacg ggattctcga
     6781 tcaggcgcgt gacgtattgt ttgctcagct gcacggcttc gtcgagctcg cgaccattgg
     6841 cgaggtaggt ggcgattgcc gacgagagcg cgcagccggt tccgtggaca tcttcgccgc
     6901 ctagaatgcg cgggctgcgg agctcgatca tcgggccggg attgcccgcg tgaatgagga
     6961 catcgaccga ctcgtcaccg ccgaggtgac cacccttgat aagaacggcc gtgtcgagct
     7021 tgcgcgcgag cgtgcgtgcg gcggcttccg ccatcaccgg cgtgcctgtg ggaaggcccg
     7081 tcatcaggaa gagctcctgt ttgttgggcg tcacgagcgt gaggtgtggg cgaagcgcaa
     7141 gcatcacctc ttcgatgtcg tcggtcagcg gagcgttacc gcgagacgcg aacatgacgg
     7201 ggtcccagac gaccggtgcg gcgctgaggt gcagcgcgtt tgcgatcgcg cgtgcgatag
     7261 cggctgagcc gaccatgccg atcttgatcg ctttcatctc gacgtcggtc atcaggaatg
     7321 cgagctgatg gccgacgacg tctggatcgc acgggtgcga gccgaccacg cccgtcgtgt
     7381 tctggatcgt cagtgccgtg acgacgccac acgggcgagc gccgagctca ctgacgacgc
     7441 gcacgtccgc gacgagacct gctccaccgg atgggtcgag acccgcgcac acgaggacgt
     7501 cgggcacgcg tgtgttggca gccgcgcggt acggcgacat atcagccgtc gaacgcggcg
     7561 aggcccgtca catcttgacc gacgacgagc gtgtggatgt cgtgcgtgcc ttcgtacgtg
     7621 tacacgctct cgagattgag catgtggcgg ccgcactggt actcgtcggt cacgccgttc
     7681 gcgccgagga tgtcgcgtgc atcacgcgcg cagtcgcgag ccatcgccac gcagttgcgc
     7741 ttcgcgagcg agatctgggc gggcgtcagc ttgttcgatt ccttgagacg accgagccgc
     7801 cagcacagca gctgcgcctt cgtgatctct tggaccatat cgacgagctt gcgctgcacg
     7861 agctggtagc ccgcgatcgg ccggctgaac tgcacgcggt ctttcgcgta cgacagcgcc
     7921 tcgtcgtagc aagccatcgc cgcgccgacc acgccgtagc agatgccgaa ccgcgcttgc
     7981 gacaggcacg acagcgggcc tctcatgccg gacacgcctg tcagcagcgc ctcttcaccg
     8041 acgacgcagt cctccatgat cagctcgctc gtcaccgaag cgcggagcga ccacttgccg
     8101 tgaatgtcgc gcgccgtgaa gcccttcgtc ttcgtcggaa cgaggaagcc acggacaacg
     8161 ccgtcgagct ttgcccacac aagcgcgagg tgcgcgaccg agccgttcgt gatccagcgc
     8221 ttcgtgccgt tgaggcgata gccgccgcct tcgcgcgtcg cggtcgtgag catcccgttc
     8281 gggttgctgc cgaaatcggg ctcggtcaga ccgaagcaac cgatcacgcg accggcggcc
     8341 atctcgggca gccacttttg cttctgctcc tcggagccga acgcccagat cggatacatc
     8401 acgagcgagc tctgcaccga gcagaacgag cggatgccgg agtcgccacg ctcgagctct
     8461 tggcaaatga ggccgtacgc cgtcggggag atgccggcgc agccgtagcc cgtgagcgat
     8521 ggaccgagca ggcccatctc gccgaacacc gagacgagct cgtgcgggaa cgtgccggcc
     8581 gagaagtgct tgccgatcga cggcatcacg cgcgagctca cgagctcccg aacggtgtcg
     8641 cgcaccatgc gctcttcgtg cgtgaacagc tcgtcgaggg cgtagtagtc gagcgcggcg
     8701 tacgtcatgg ttgcgcgggc gttatacgac gaaaacgcta tactcgtagg cctcgtgagg
     8761 gtgctcgttc tcctagtcct tctcgcgtct tcggttgcgc gggcggacgc tccgaatgcg
     8821 aagaagctcg gcgaggcgta cgacgcctac ggagcgagcg atctcgcgaa ggccaagaag
     8881 ctgctcggct cgctcgacga caccaagctc gtcgccacgg actacgcact ctggctgcgc
     8941 ggcatggttg cgttgcgcgg cggagatccg gcgaccgcgc agaaggcgtt cgagaagctc
     9001 gcaaagatga agggcacgtc gttcgcggct gaagtgccgt ggcgactcgc cgattgcgcg
     9061 tgggagaaag gcgacaagac tggcgccgcg aaggcgtacg cgaagctcat caagagcggc
     9121 aacgcgagcg acaccggcga catcggcacc gcgaagttcc gcatcgccga ggcaaatccg
     9181 agcgcagcag cgtatcgcgc tgtcgtgatc gatcatccag cacatccact cgcggcgaaa
     9241 gccgaagcgc ggcggctcga gctcggcggc agcccgctca cgtccgagga tcgcatcgag
     9301 cgcgcgaagc agctcaacgc ggcacacctc tgggaccagg cgatcgtcga gctcgcactc
     9361 gtaccgcaga ccggcgtctc cgactcgctc gcggcacagc gcgactactg gaccggcaac
     9421 acgctgttcg agatgcgccg gcgttacgcc gatgcggcga cgctcctgct cggcgtgtac
     9481 gagaagctca actccgccga agcgatgttc cacggtgcac gcgcactgtc tcgcgcggac
     9541 aaagacgatg aagcgatcgt ctggtacggc aaggtcgtcg acaaatatcc gcgcacctcg
     9601 tgggcagaag aagcggcgtt cttgcgcggg tggctggagt tcaatcgcgg ccactacaaa
     9661 gaggcgatca agccgctcga ggaatcgctc gcgaagtatc cgaagagcaa gtgggtcgac
     9721 gatggccact ggtttctcgg catgtctcac tacttcctcg gcgagtggga cgccgcacgt
     9781 accgagctcg gcaagctcgc gaagttctcg ggcgcgctcg aaggcggcaa gggcacgtat
     9841 tggctcgcgc gcatcgacga gaagctgaag aagaacgact ccgcgatcgc aggctacaag
     9901 gacaccgtca gcgatcatcc gttctcgtgg tacgcgctgc tcgcacgggc acgactgggc
     9961 cagctcggtg tgaagatcgg gccgttcggc gtcgagaatc cgcagcccaa aggcggcaag
    10021 ctatccgcga ccgtcgacga gaagctcgcg accgacgagc tgatcaagaa ggtcgacgag
    10081 ctgatcaccg cggacatggg cgtcgatgca ggcaacgagc tcgtgcgggg cgagaaggct
    10141 ttcctgaagc gacacgatcg cggctctgcg ttcgcgatgc tgctcgatcg ctatcgcaag
    10201 gccggcaact tcaacaggcc gtggctgctt gccgtgatct acggaggcag cgcgctcgat
    10261 gggccgccag aaggtgatgc gcggcgctgg tgggagaatg cgtatccgcg cgcgtatcgc
    10321 gacctcgtcg agaagtatca ggacctcggc aagaatcccg agggctatct ctattcgatc
    10381 atgcgcaagg agagcggctt caacccgcac gacgtctcgt acgccgatgc gcagggcctc
    10441 ctgcagatga ttcctccgac gacgatgcgc gtcgcaaagg ttctcggcat tccgtacgac
    10501 cccggcaagc tgtacgagcc cgagtacaac atccacatgg gcggctggta catcggccat
    10561 ctgctgcaga agttcaaagg ccagattccc ttcggcgccg gctcgttcaa cagcgggccg
    10621 cggcccgtca tgaagtggct cgatctctac ggcgatcgcg acgtcgatga agcggtcgag
    10681 ctcgtgccgt acacgcagac gcgcgagtac atgaagaagg tgacggagaa ctacgcgcgc
    10741 tatcgctacc tctacaacaa cgaggtctac gaccagccgc tcaccgtcga caaggcgtat
    10801 ctcaaggacc agctcaccta ctagaagagc tcgcccacta gaagagcggg tctccgatcg
    10861 gcatcgctgg gatgcgattg acctgcgcga agtcgagctg cagctcgtcg gggacgtcgc
    10921 acatcgttcc cgcgcgcgag cggtcgccgg tcaccttcgc gatgtcgccc ttgatcgcga
    10981 gcttgccgcg aaacgtttcg gtggcgcgac catcgaggag acgctcgcac gacgtggtca
    11041 cctcggtgca gcggtacgtg ccgccgtcgc agaacacgct gactttgcgc cgcacctcgt
    11101 tcgctgacga tgtctcgtcg ctcgtctcgc tctggacgac gccgacgtcg aagcggtagc
    11161 cggtgtgctt cgcgatcgtc gggcgcgaga agccgcgtac gtcgaaggtg ccgatcgctt
    11221 cgaacattcc tgcgatcacc cagcgtttgc cgacggagcg atagagatac agacccggca
    11281 cgttgaactt cgggtacgcg attccgacga gctttgctcc atcgacggag cgctccagct
    11341 tcggcgtgcc gaaacgctcg aggcacttca tgaccttgtc ccagctctcg gcggtgccac
    11401 acgtgcgagg aaccgtaggc gtcatcacca cgcgcgccgc cgcgctgctc gtcagtgcca
    11461 gcacgatcac gatcgcgagc ttcattcgag gatgtcgccc acgttcgaca gctgtgacat
    11521 cgcgggcgcg cagacctggc ccgcatacgc gcgatcaccc gtcatgcgta cgacgccatg
    11581 atcgatgagc agcgtgccgc gaaagctcca gcgcgtcttg ccatcgacga gcgcatcgca
    11641 cgatgtgatg aacgtctggc agtaaccgtt atcgaggcag atcgacgttg tcttgcgtcg
    11701 catcgtgacg ggcgtccgcg tctggtcggc acctggaaag cgttccgtgc tgaatgccgt
    11761 cgacttgaag atctggccct gctctagccg atagccgttc tctttcttca gccgctcgaa
    11821 gctcaagatc tcgctcgtgg cgttcgtcgc gaagtagcca gacatccgct gccagcgtcc
    11881 gtcctgcttc gtgtagatga acaccgacgt ggagtcgtag ctcgggttgc tcttcaccga
    11941 tacgaggcgg cccttgccga ggtcgcgcat cgtggcgacg gtattgccgc cctgcgcgag
    12001 acacttcacg agcttgggcc agctcttgtt gcgctcgcac agctcctcgg gcgtccggac
    12061 aggctgcagc ttgcgcttgc ttcccttccg acgaatcagg tcatcccgat cgaacggctc
    12121 gtcgaaggga tcgcgcaccg gatgcgcgaa cgcgacgccc gacatcagga tgctcgccaa
    12181 cacggcgcaa accacccgca cgactcgatt atgacccgac cggtcgtttg aaacgagcat
    12241 gctaatcttc aacgcatgaa gctcaaaggc gtgttgacgc actcggatct cgagggaggt
    12301 cactggctcc tcaagaccga cagcggtgac gagtaccagc tgaccggcgc catcaaggac
    12361 tgcaaggatg gccagcgtgt cgaggtggag ggcaaggtcg acaagcaagc catgggcatc
    12421 ggcatgatgg gcccgcactt caacgtctcg aagctgacgg cgctctgata cgctagagcc
    12481 gtggggctcg cgatcaccga tttcgccacc tcgaccggcg cgttacgcga catctacgat
    12541 cggatgcgcg agcggccgct ccctccggca taccgtccgg cgcatggcgg cgtgcctggg
    12601 atcatcacgg cgcacagcct cgatccgcag ttgatcggca aagtcttctc gacgtcgacg
    12661 acgctcaacg gcgcgggccc actcacgtgg ccggaacgcg agctcgtcaa cgcgacgacg
    12721 tcacgtctca atcaatgttt ctattgaacg gcttgtcacg cagagtttct gcgtgtcgcg
    12781 ctcgataacg acgacgcgct ctctcgcgcc gtgatcgccg acgctcgcca cgtcgcgcag
    12841 cacgccccga acgcgcggat gaagatgatc gcggagctca cgacgatcgt gaccgaagcg
    12901 ccgtggacgc tgtcgcgctc ggtcctcggg cgcgcccatt ccgccgggct ctccgacgaa
    12961 gacgtcctcc acgtcgtcct actttcgtcg tacttcggtc acctcaatcg catcgccgac
    13021 gcgacgggcg taccgctcga ctacaacgtc aagctagagg ttccggcgac ggatccggcg
    13081 tgcccgccat ggccagcagc tccgagcctg atcgccggac gccacgcgat cgatatcgct
    13141 cgccgtgctg caacggccac ggcattgtcc gactggcgga cgtacatctt ctatcgcgac
    13201 gcgccgctca cccgccgcca acgcacgctg atcgcccgct gggtcgcaac ctggctcggc
    13261 gatggcggca tctcaccgcc gaccgatctg acggtgaacc cgatcgacga ccacctgcgc
    13321 gcagtcgccg aagtcgtcac gctcgcgccg tggaagatca ccgacgcaac attcgcgccg
    13381 cttcgcgagt ccggcttcga cgacgccggc atcttcgacg tttgcgcgac agcaacctcg
    13441 gccggcgtgt tctcgcggat cgaagtcgcg ctcgtcgcgc tcggtcacta accgcggagc
    13501 gaagcttgtt cgtccgggac ccggacaaac cacctccgga ttcacttacc ccggtcatct
    13561 ttgacgcgcg tcaaaaactt accccggtat tgccgatgcg cgcgtagccg cggcctttgc
    13621 ttccggattc acttaccccg gtagttttcg aggcgagaca agctcgcgcg attgcagcaa
    13681 aaggtgctgg cgagtcagcg agcgccgacg agctagagcg agctcgagct tcagcgagcg
    13741 ccgtggcgag ctagagcgag ctcgagcttc agcgagcgcc ggcgagctag agcgagctcg
    13801 agccagcggg cgccggaggg ggcgcaggcg tcggagcggg ggtcggcgtg gtctcgccac
    13861 ctgcgggctt gtcgccacca gcgggtgtcg cggcgctgcc tgtgcctttg tcgccttggc
    13921 cgccagccgg acgctggacc gggcggcgga caggacgcgt ggctttgggc gtcggcggct
    13981 ttgcggcctt ctcttcgccg gcgcctttgc cttctccgtt ggagccggtg ttcgggccgc
    14041 cactgccggt gcctgcacca ctgccggttt cgggggttgc gccgccagcg gcgccggagc
    14101 cggtgcccgc ggcagaacca gcggagccgg tgtcacccgt cggcggggtt tcggtcggcg
    14161 tttcgccggt tgcggggcgg ctggagtcga gaccggcgaa cgtgacttcc gtgccttcgg
    14221 cggcggcgcc ggacggacac gcgatcttcg ttccgtcgcc agaggcgagg acgacgttgc
    14281 acgcgagctt ttgatcgctg cctttgacgg cgagcaacac cggcgcattg tccgtcgcgc
    14341 cgtctgcttt cttgaacgtg gcaacgagga tcgcgtcgcg ctgcagcgtc gcgacgacct
    14401 ccgtcggcgt aaccttcgcg ttgatcttcg cgacggccgt gaccttgccg gcggccggcg
    14461 ccttgagcag gaacttgtcg agcgcgccct gcttgtcggc gagcttcttc tcgttatcgg
    14521 cttgggcttt ttgcgcggcc gcgagcttct tctcgtcgct gcctgcagcg gcgacatcgg
    14581 cctttgcctt gtcgagcgcc gtcgtgatgt tcttgacgtc cgcttcgagc gacttgaagc
    14641 cgacgtattt cgcgacgggg tcgcccgctt tgacgacggt gtcattcgcg gcgatccacg
    14701 cgagctgatg cgcatcggtc ggcttgaggt cttcgggagc cggctgctcg acggcgagct
    14761 tctgcgactc gaccggcggc ggaggcggtg gctctggctg cttcggcgtc gtgaccggcg
    14821 tgttcgtcgt cgactccgtc gtgtccttct tcttgaggac gaacttgtag agtgcgaaac
    14881 caccggcggc gaggatcgcg atgacgagcg cagcgatcag cacggggcta acgccttgac
    14941 gcggcgcaac cggcgcgacc gacggacgct tctcgacggc aggagacttc gacgcggcgg
    15001 ccggcttctc cgcggccttc gcggccggct tctccgcggc cttggctgcg ggcttctcgg
    15061 cagccttcgg ctcgaccttc gcggccggct tctccgcggc cttcgctgcg ggcttctcgg
    15121 cagctgcaga cttcgacgcc gcagcgggct tcgattccag ctccgcggcg agctcgggcg
    15181 gaccgtcgac ggccttcatg acctgcgacg cacgaaccgc ttccttccca tcggccgcac
    15241 cgatatcgac gtggtcggcg agatcgacgg acacgttgga gacatcggcc gagcggcccg
    15301 aggcaccgag cgcgatgtcc gcgccctttg ctgcagcagc gcccttcgcg cctgtccgcg
    15361 cgcccggcgc accgagctcg agatccggcg cgttcggcgc atcggcttgg acttcgacgc
    15421 cacgcgcacc gggtgcaccg agctcgacgt ccgcgcccga cggagcgggc ttcttcgcgc
    15481 caccggcgac gacgccttgc gcgag
//
LOCUS       DATLBZ010000618         4417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3518660, whole genome shotgun sequence.
ACCESSION   DATLBZ010000618 DATLBZ010000000
VERSION     DATLBZ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3518660"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VMZ53_30415"
     CDS             <1..670
                     /locus_tag="VMZ53_30415"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HUS32869.1"
                     /translation="DDWYKVVLAADSRVNFEIAGDGASDLDLHLYHSDGTVVSASTSY
                     SSDENLTTCLKAATYYVKVNGYGGARSEYLLSYDKTAESCDTTCVDDSAEDDDTYSQA
                     RAAAYPTHTATAQKICKNDDDWYKVRLYAGEKITMDLTFTQSTYTQDLDLHLYKDFTD
                     LWPCSPTDVAGCTSAHGQGAVSNEHAIYTVPTGTCASGCDYYVVVRGWNGGSNSYGIN
                     LKVE"
     gene            670..2607
                     /locus_tag="VMZ53_30420"
     CDS             670..2607
                     /locus_tag="VMZ53_30420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32870.1"
                     /translation="MRFPLGSFFAVAALVGCASSTASDPGLEGLSIEKVAPDTIIPGS
                     KIVVKGASFVDEQWGSASLRIVGKAGGTNVDLTWPAKFVDFNTLTVAVDGGKIDSLGG
                     DVDFAGDVFIEVVATSDGDTYSTGKLTQNLFFRKKLTPSPTGVALSGVIFVNDEIEVD
                     GQGFLLGGEEGTTVAKVAGCFKLDTTTNCVAISPQEIPLQPREELSRQHAAFPFSPAI
                     AGIRPGTFQGTVQIINKHAGGASVTADPITTNASLVTSQVFSADPPNASLGQYVFVHG
                     GGFVGGENGALTTLKLTGTFNKTGMNPAPITMYLIPEFVDGRLVRYVLNTDDELGTSL
                     DLFKDTGKFTGSIQPIIQYGGQTVQGVSSQVTFNIAPVKQIVYLEFQPSYVEGLRDFG
                     LRAVDKKIRERILAELARIYKGINIEFRTEAPTDYALYEYVTLVGVDPNNMGLFGYDN
                     SPGKDNGNVRLYDKLGGVNAVTQQDGYPGFGGVFLRSLMGFSKHPGSFAKSVPGADPV
                     FDKIFDTFRPDQDGTPVSSSDLAGGLTALTSGDQCPGSDRKAKLECAVFVLGNLVGGT
                     LGHEIGHSLGLANPYQEGFHDAGDAPARLMDAGGDRPFMERAALQGTQPAVFCDDEYD
                     YLRQILPSKDPADTVARPSCY"
     gene            2682..2957
                     /locus_tag="VMZ53_30425"
     CDS             2682..2957
                     /locus_tag="VMZ53_30425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32871.1"
                     /translation="MYRLVLLVVMIPACWAEDGRVCHYQDSSYAEDVFDQTCQGVTTT
                     YVSRGCNFDDDWWSQRDCENACPVKATCKLKERRGSYGGYCTATICS"
     gene            2975..3415
                     /locus_tag="VMZ53_30430"
     CDS             2975..3415
                     /locus_tag="VMZ53_30430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32872.1"
                     /translation="MERRRDLRPYIYLALDQIFVIAYFYLVIAAIPNRLPTAKINLYA
                     IPVILQLVALGMATIFVPSLRKIGWWVVVVGQSLLLAVTVLLIIRVLISAAFLSGTYG
                     AFGKAASMTALMGVAIVVEVVGLLPLFQIKFMRTKAGRRAYAEP"
     gene            3412..>4417
                     /locus_tag="VMZ53_30435"
     CDS             3412..>4417
                     /locus_tag="VMZ53_30435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32873.1"
                     /translation="MKRWHVLVLVWTLPSVVFLAITAVVYSAYRIDPPERLSDAERAA
                     VMKPLREALEDRAPPACTVKRAGRAVAVTVWVAGRMIGRVDGYGADLANAVDDAARQL
                     KVHPQVRSLPLTLQQQAKMQVDVIIGRAALGDQHWLGQYAIPFVTGMLPVNPGVDGIG
                     AEHDGKKALLLPHELVHSRVLSGQVKAETIDFSMGVDMKRIAVYISSKMGRQVPSSAL
                     FRFRTDTFVEAPDLAKPPVQLTRGIPPPPPLTGQALREAALAGGRYLVAHLSPSGRYI
                     YEHDLTTGQKTDPRSGAYSMPRHAGTTYFLSELYRITKEPWLREPIERAFAHLAELLS
                     S"
BASE COUNT          781 a         1427 c         1419 g          790 t
ORIGIN      
        1 tgacgattgg tacaaggtcg tcctcgccgc ggactcgcgc gtgaacttcg agatcgccgg
       61 cgacggcgcg agcgacctcg acctgcacct ctatcactcg gacggcacgg tcgtgtcggc
      121 ttcgacgagc tacagctcgg acgagaacct gacgacgtgc ctcaaggcgg cgacgtacta
      181 cgtgaaggtc aacggctacg gcggcgcgcg cagcgagtat ctgctctcgt acgacaagac
      241 ggcggagtcg tgtgacacga cctgcgtcga tgactcggcc gaggacgacg acacgtacag
      301 ccaggcgcgc gctgcggcgt atccgacgca caccgccacg gcgcagaaga tctgcaagaa
      361 cgatgacgac tggtacaagg tccgcctcta cgctggcgag aagatcacga tggacctgac
      421 gttcacgcag tcgacgtaca cgcaggacct cgacctccac ctgtacaagg actttaccga
      481 cctctggccg tgcagcccga ccgacgtcgc cggttgcacc agcgcccatg gccaaggcgc
      541 cgtctcgaac gagcacgcca tctacaccgt accgaccggc acttgcgcct ccggatgtga
      601 ctattacgtc gtcgtccgcg gttggaacgg cggatcgaac tcgtacggga ttaatctcaa
      661 ggtcgagtaa tgaggtttcc actcggctcg ttcttcgccg tagccgcgct ggtcggctgc
      721 gctagctcca ctgcatccga tccgggcctc gagggcctct cgatcgagaa ggtggcaccc
      781 gacacgatca ttccgggctc gaagatcgtc gtgaagggcg cctcgttcgt cgacgagcag
      841 tggggctctg cttcgctgcg catcgtcggc aaagcgggcg gcacgaacgt agacctgacg
      901 tggcccgcga agttcgtcga cttcaacacg ctgaccgtcg cggtcgacgg cggcaagatc
      961 gattcgctcg gcggtgatgt cgacttcgca ggcgacgtgt tcatcgaggt cgtcgcgacg
     1021 agcgatggcg atacgtactc gacgggcaag ctcacgcaga atctgttctt ccgaaagaag
     1081 ctcacgccgt cgccaacggg cgtcgcgctg tcgggcgtca tcttcgtcaa cgacgagatc
     1141 gaagtggacg ggcagggctt cctgctcggc ggcgaagagg gcacgaccgt cgcgaaggtc
     1201 gccggctgct tcaagctcga caccacgacc aactgcgtcg cgatctcacc gcaggagatt
     1261 ccgctgcaac cgcgcgaaga gctgtcgcgc caacatgcgg cgttcccgtt ctcgcccgcg
     1321 atcgcaggca ttcgccccgg cacgttccag ggcaccgtcc agatcatcaa caagcatgca
     1381 ggcggtgcgt cggtcacggc cgatccgatc accacgaacg catcgctcgt cacgtcgcag
     1441 gtgttctctg ccgatccgcc gaacgcatcg ctcggccaat acgtgttcgt gcacggcggc
     1501 ggctttgtcg gcggcgagaa cggcgcgctg acgacgctca agctcacggg cacgttcaac
     1561 aagacgggca tgaacccggc gccgatcacg atgtacctga ttcccgagtt cgtcgacgga
     1621 cgcctcgtcc gctacgtgct caacaccgac gacgagctcg gtacgtcgct cgatctcttc
     1681 aaagacacgg gcaagttcac tggctcgatt cagccgatca tccaatacgg cggccaaacc
     1741 gtgcagggcg tgtcgtcgca ggtcacgttc aacatcgcgc ctgtcaaaca gatcgtctat
     1801 ctcgagttcc agccgtcgta cgtcgaaggc ctgcgcgact tcggcctgcg cgccgtcgac
     1861 aagaagatcc gcgagcgcat cctcgccgag ctcgcgcgca tctacaaagg catcaacatc
     1921 gagttccgca ccgaagcgcc gacggactac gcgctctacg agtacgtcac gctcgtcggt
     1981 gtcgacccta acaacatggg cctgttcggc tacgacaact cgcccggcaa ggacaacgga
     2041 aacgtccggc tctacgacaa gctcggtggc gtcaacgcgg tcacgcagca ggacggctat
     2101 cccggtttcg gcggcgtgtt cctgcgctcg ctgatggggt tctcgaagca ccctggttcg
     2161 ttcgcgaagt cggtgcccgg tgccgacccg gtgttcgaca agatcttcga cacattccgt
     2221 cccgatcaag acggcacgcc cgtcagctcg tcggatctcg ccggtggcct gacggcgctc
     2281 acgtccggcg atcagtgccc gggcagcgac cgcaaggcga agctcgagtg cgcggtgttc
     2341 gtgctcggca atctcgtcgg cggcacgcta ggtcacgaaa tcggccattc gctcggcctc
     2401 gcgaacccgt atcaagaggg cttccacgac gcaggtgatg cgcccgcgcg gttgatggac
     2461 gccggcggcg atcgcccgtt catggagcgt gcagcactgc agggcacgca gcccgctgtg
     2521 ttctgcgacg acgaatacga ctacctccgc cagatcctgc cgtcgaagga tccggccgac
     2581 accgtcgcgc gcccgagctg ttactaaccc gcgaggtcgt cctcatcgtc gctgccggcg
     2641 attgccgcgt ggcaccagcg atcccgcgat acgatgggct catgtatcgt ttggtacttc
     2701 tcgtcgtgat gataccggcg tgctgggccg aggacggtcg cgtctgccac taccaggaca
     2761 gctcgtacgc cgaggacgtg ttcgaccaga cgtgccaggg cgtcacgacg acgtacgtgt
     2821 cccgaggatg caacttcgac gacgattggt ggtcgcagcg agactgcgag aacgcgtgcc
     2881 ccgtgaaggc cacctgcaag ctcaaagagc gccggggaag ctacggcggg tactgcaccg
     2941 caacgatctg cagctagtca ggtaagctcg ctcggtggaa cgccgccgcg accttcggcc
     3001 gtacatttat ctcgcgctcg atcagatctt cgtgatcgcg tacttctatc tcgtgatcgc
     3061 ggcgattccg aatcgcttac cgaccgcgaa gatcaacctg tatgcgattc cggtgatctt
     3121 gcagctcgtc gcgctgggta tggccacgat cttcgtgccg tcgctgcgca agatcggttg
     3181 gtgggtcgtc gtcgtaggtc agtcgctgct gcttgcggtg accgtgctgc tgatcattcg
     3241 cgtgctcatc agcgcggcgt tcctgtccgg gacgtacggc gcgtttggca aagccgcgtc
     3301 gatgacggcg ctcatgggtg tcgcgatcgt cgtcgaggtc gttggcctct tgccgctgtt
     3361 tcagatcaag ttcatgcgga cgaaggcggg gcgacgcgcg tacgccgagc catgaagcgc
     3421 tggcatgtgc tcgttctcgt ctggacgctg ccgagcgtcg tgttcctcgc gatcacggcg
     3481 gtcgtctaca gcgcgtatcg gatcgatccg ccggagcggc tgagcgacgc cgagcgagct
     3541 gccgtgatga agccattgcg cgaagcgctc gaagatcgcg cgccgccggc gtgcacggtg
     3601 aagcgagcgg gcagggccgt ggcggtgacc gtgtgggttg cgggtcgcat gatcggacgc
     3661 gtcgatggct acggcgccga cctcgcgaat gccgtcgacg atgcggcgcg acagctgaag
     3721 gtgcatccgc aggtgcgctc gctgccgctc accctgcagc agcaagcgaa gatgcaggtg
     3781 gacgtgatca tcggccgcgc ggcgctcggt gatcagcatt ggctcggcca gtacgccatt
     3841 ccattcgtga ccggcatgct gccggtgaat cccggcgtcg acggcatcgg cgccgagcac
     3901 gatggcaaga aggcgctgct gttgccgcac gagctcgtgc attcgcgcgt gctctcgggt
     3961 caggtgaagg cggagacgat cgacttctcg atgggcgtcg atatgaagcg catcgccgtc
     4021 tacatcagct cgaagatggg tagacaggtg ccgagctcgg cgctgtttcg gtttcggacc
     4081 gatacgttcg tcgaggcgcc ggatctcgcg aagccgccgg tgcagctcac gcgcggcatc
     4141 ccgccgccac cgccgctgac gggccaagcg ctgcgcgaag ccgcgctcgc cggcggccgg
     4201 tacctcgtcg cacatctctc gccgtcgggc cgctacatct acgagcacga tctgacgacg
     4261 gggcagaaga cggatccgcg aagtggcgcg tactcgatgc cgcgtcatgc gggcaccacg
     4321 tacttcttgt cggagctgta tcggatcacg aaggagccgt ggctgcgcga gccgatcgag
     4381 cgtgcgttcg cgcatctcgc ggagctgctc tcgagtg
//
LOCUS       DATLBZ010000619         5606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7228558, whole genome shotgun sequence.
ACCESSION   DATLBZ010000619 DATLBZ010000000
VERSION     DATLBZ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7228558"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VMZ53_30440"
     CDS             complement(<1..196)
                     /locus_tag="VMZ53_30440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32874.1"
                     /translation="MAPSSPSDPGGHDRLRRCVRDLSALTGLPSLCVGRTPDEALDTL
                     VDALPTALSCDLVYLMLPGAP"
     gene            complement(203..811)
                     /locus_tag="VMZ53_30445"
     CDS             complement(203..811)
                     /locus_tag="VMZ53_30445"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HUS32875.1"
                     /translation="MSLATSLAHAQLRNHFHACAFVANEEEERAVIDPFLLEGLRKRE
                     KAVYIVDPDHRERHESRLKAGAPDNHDLVDVTTWHQAHLKGGTFDQDRMMQGLEDLIR
                     DHAATGAPPMRLVGQMGWIAKKPPGIEQLVAYEATVNEVLNRGKTPTVCVYDVRKLSG
                     SWMRDLLRAHPLTVMNGVLHENPFYTPADEMLAELKHRCPAN"
     gene            complement(964..2301)
                     /locus_tag="VMZ53_30450"
     CDS             complement(964..2301)
                     /locus_tag="VMZ53_30450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUS32876.1"
                     /translation="MSLALIISDAVASPELIIVSGELVRGALSAAGFSQIEKVRSIHE
                     ASAVFAKLQPDLVVLDMVEPQTFESYRRLIARLRGTPLLAILAEEQMGAAFDAGIDDC
                     LARPVRTTELVARARAAIRLRIERVRRTRRDRRLSEELRVLQEEKHELERIACVDSLT
                     GVANRRHTMTLLDAEWKRSLRDGTPLALVIADIDHFHAYNETYGHPGGDACLRKVTAE
                     IATCLRRPSDFVGRYGGEEFIAVLANTDASGARIVAERVRSSVEQLAIPHSTSSCSRY
                     VTISVGFASILPKSEQGADALLKAADSALLRAKASGRNRISGDAPTTTARPRISSHPW
                     RRFPAVVLDPWYAEKVPTFLATTRNEIEVLREALELGALDRIRAVARRLRGGAGDHNI
                     PTIGELANLLDRAARSDDREGIGRVIDELAQYVDHVVVQYRSPAERKLGQVGS"
     gene            complement(2421..3830)
                     /gene="lpdA"
                     /locus_tag="VMZ53_30455"
     CDS             complement(2421..3830)
                     /gene="lpdA"
                     /locus_tag="VMZ53_30455"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HUS32877.1"
                     /translation="MSGFDVIVIGSGPGGYVAAVKCAQLGLKTACIERAELGGICLNW
                     GCIPTKALLKSAEILEHAQHAKDFGILVDNPRPDFTAIIKRSRDIADKISKGVAYLFK
                     KNKLESIAGTAKLLPRSGPWKPLQVQVDQAGGGQVVLDTKHVIIATGARARSLPGIEL
                     DGERIIEYRKAMTLAQQPKSMVVLGSGAIGVEFASFYRALGVDVTIVEYLPRLVPNED
                     PDVSKELGKAFEKKGIKILVGHKLTTAKNAGDRVVMSVEPAQGGEKKELVADVLLVAV
                     GIAANTENIGLEAHGITLEKGFIKVDSENKCGDGLWAIGDVAGRGLAHVASAEGVFVA
                     EKIAGVHAEPVRYDAIPACTYCHPEIASVGLTEEKAKAQNIPLKIGRMPWRPLGRAMA
                     SGEATGFVKVLWHGETGALVGAHMIGPAVTDLIAELTLAKTTEVNAESLIYTIHAHPT
                     FAEAVRIASEDALGIAVDL"
     gene            complement(3827..5239)
                     /locus_tag="VMZ53_30460"
     CDS             complement(3827..5239)
                     /locus_tag="VMZ53_30460"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HUS32878.1"
                     /translation="MAQIIGLPKLSPTMEEGVLVRWTKKEGDKVSPGDLVAEVETDKA
                     NMDFNIEDEGVLLKLLVKEGDTVKLGAPVAIIGKAGEDVSALVDQAKSGGGGAAPAAK
                     QEAPKQSAPPPKGPEVSTSAPAAKKETPAQKAPAPKAAQPAAKPAAQASGGKLLASPL
                     AKTLAIELGIDLRTIQGSGPGGRIVERDVKAAAEGGEGGSNGHAQEAKAGEPQEAEQD
                     DDIESRTTQALAVRHARQMTPEAEGDYEDIPASNMRKTIAARLTEAKRDVPHFYLMRT
                     FDAAPMMSFRARLNELLGDKGKVSVNDLIIKAVALALRRVPECNAQWHGNAIRKFHRV
                     HIGVAVALDEGLITPVIRDADQKGIGSIAAEVRDLADRAKNKRLKGHEISGSTFSVSN
                     LGMYNIERFTAIINPPEAGILAIGTVVDTPVVKGSEVVAGKRMTVTMSCDHRVIDGAL
                     GARWLAAFVDLIEHPESLAL"
     gene            complement(5243..>5606)
                     /locus_tag="VMZ53_30465"
     CDS             complement(5243..>5606)
                     /locus_tag="VMZ53_30465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HUS32879.1"
                     /translation="IAWSKMVWTCLDAAKVLAEEGIECEVVDPRTLRPLDVDTLAESV
                     RKTGRCVIVEIGWPVAGFGAEIAYQMQKHCMDSLDAPIERVTSDDVPMPYAKNLEDEV
                     QPQIKDVVAAVRRVLYLD"
BASE COUNT          895 a         1833 c         1830 g         1048 t
ORIGIN      
        1 gcggggcacc cggcagcatg aggtagacca ggtcgcagct gagcgccgtc ggcagcgcgt
       61 cgacgagggt gtcgagcgcc tcgtccggcg tgcggccgac acagagcgac ggcaaccccg
      121 tgagcgccga gagatcgcgg acacagcgcc ggagccggtc gtgtcctccg ggatcgctcg
      181 gcgacgaggg agccatccgg cgtcagttcg ccgggcagcg gtgcttgagc tcggcgagca
      241 tctcgtcggc cggcgtgtag aacgggttct cgtggagcac gccgttcatc acggtcaacg
      301 ggtgcgcgcg cagcaggtcc ctcatccacg aaccgctgag cttgcggacg tcgtagacgc
      361 acaccgtcgg cgtcttgccg cggttgagca cctcgttgac ggtggcttca tacgcgacga
      421 gctgctcgat gcccggcggt ttcttcgcga tccagcccat ctggccgacg aggcgcatcg
      481 gcggtgcgcc ggtggccgcg tgatcgcgga tgagatcctc gaggccttgc atcatgcggt
      541 cttgatcgaa cgtgccgccc ttgaggtgcg cttgatgcca cgtcgtcaca tcgacgaggt
      601 cgtggttgtc cggcgctccg gccttgaggc gcgattcgtg acgctcgcga tgatcggggt
      661 cgacgatgta gacggccttc tcgcgcttgc gcaatccctc caagagaaac ggatcgatga
      721 ccgcgcgctc ctcttcctcg ttggcgacga acgcacaggc gtggaagtgg ttccggagct
      781 gagcgtgagc gaggctggta gcgagggaca tgcagacgac gctaacagcc ggtatcgatg
      841 cgcggaagca aagaacgatg ctctacgtct cgcttaacaa ttcggaatcg cccgattcga
      901 gcgcacgcga cacgccgagc gcatgcgcac gcgatcgcca aagggaatac cttcgacgac
      961 tacctagctg ccgacttggc cgagcttgcg ctccgctggg ctgcggtact gcacgacgac
     1021 gtggtcgacg tactgcgcga gctcgtcgat cacgcgaccg atgccttcgc gatcatcgct
     1081 gcgcgcggcg cgatcgagga gattggcgag ctcgccgatc gtcgggatgt tgtgatcgcc
     1141 ggcgccgccg cgaagacgac gcgcgaccgc ccgaatgcga tcgagagcgc cgagctcgag
     1201 agcttcacgc aggacttcga tctcgttgcg cgtcgtcgcg aggaacgtcg gaactttttc
     1261 cgcgtaccac gggtcgagca cgacggcggg gaagcgacgc caggggtggc tcgagatgcg
     1321 aggacgtgca gtcgtggtcg gagcatcgcc gctgatgcga ttgcgacccg acgctttcgc
     1381 gcgaagcagc gccgagtcag cggctttgag caacgcgtct gcgccttgct cgctcttcgg
     1441 caggatcgac gcgaagccga cgctgatcgt gacgtagcgc gagcagctcg acgtcgagtg
     1501 cgggatcgcg agctgctcga cggagctgcg cacgcgctcg gcgacgatcc gcgcaccact
     1561 ggcgtcggtg ttcgcgagca ccgcgatgaa ctcctcgcca ccgtaacggc cgacgaagtc
     1621 cgacggccgg cgcaggcacg tcgcgatctc cgccgtcacc ttgcgcaagc acgcgtcgcc
     1681 gccaggatgg ccgtacgtct cgttgtaggc gtggaagtga tcgatgtcgg cgatgaccag
     1741 cgcgagcggc gtaccgtcgc gcagcgaccg cttccactcg gcgtcgagca gcgtcatcgt
     1801 gtggcgacga ttcgcaactc ccgtcagcga gtcgacgcac gcgatccgct cgagctcgtg
     1861 cttctcctct tggagcacgc gcagctcttc gctgaggcga cggtcgcgcc gagtgcgacg
     1921 aacgcgctcg atgcgcaacc tgattgcggc gcgggcacga gcgacgagct ccgtggtgcg
     1981 aacgggacgc gcgaggcagt cgtcgatccc ggcatcgaag gcggcgccca tctgctcttc
     2041 ggcgaggatc gcgagaagcg gcgttccgcg gaggcgcgcg atcagtcgcc ggtagctctc
     2101 gaacgtctgc ggctcgacca tgtcgaggac cacgaggtcg ggctgcagct tcgcgaatac
     2161 ggccgaggcc tcgtgaatcg atcgcacctt ctcgatctgg ctgaagccgg cggctgagag
     2221 cgcacctcgg acgagctcgc ccgagacgat aatcagctcg ggagacgcga ccgcatcaga
     2281 aatgatcaga gctaggctca cggaagtgtg cacgtatcgt tcaatcgtcg tgccacaaat
     2341 cccgggaaag acctgcactt acactcaccg acgctatcgt tcacgagcgc gttcgcacgc
     2401 cgagcgctcg tgcgcgggcg ttagagatcg acggcgatgc cgagcgcgtc ttcggatgcg
     2461 atcctcacag cttccgcgaa cgtcgggtgc gcgtgaatcg tatagataag cgactcggcg
     2521 ttgacctcgg tggtcttcgc gagcgtcagc tctgcgatga gatcggtcac cgcgggtccg
     2581 atcatgtgcg cgccgacgag cgcacccgtc tcgccgtgcc acagcacctt cacaaaaccg
     2641 gtcgcttctc cggatgccat cgcgcgaccg agtgggcgcc acggcatgcg gccgatcttg
     2701 agcgggatgt tttgcgcctt cgccttttct tcggtgaggc cgacgctcgc gatctccgga
     2761 tgacagtacg tgcatgccgg aatcgcgtcg tagcggaccg gttctgcatg gacgcccgcg
     2821 atcttctccg cgacgaatac gccttctgct gatgcgacat gtgcgaggcc gcggcccgcg
     2881 acatcaccga tcgcccagag gccatcgccg cacttgttct cgctgtcgac tttgatgaag
     2941 cccttctcga gcgtgatgcc gtgcgcttcg agaccgatgt tctcggtgtt cgccgcgatg
     3001 ccgacggcga cgagcaatac gtcggcgacg agctccttct tctctccacc ttgcgcaggc
     3061 tcgacggaca tcacgacgcg atcgcccgcg ttcttcgccg tcgtcagctt gtgaccgacg
     3121 aggatcttga tgcccttctt ctcgaacgct ttgccgagct ccttgctgac gtccggatct
     3181 tcgttcggca ccaggcgcgg caagtactcg acgatcgtca catcgacgcc gagcgcgcga
     3241 tagaagctcg cgaactcgac accgatcgcg ccggagccca gcacgaccat cgacttcggc
     3301 tgctgcgcca gcgtcatcgc cttgcggtac tcgatgatgc gctcgccgtc gagctcgatg
     3361 cccggcagtg accgcgcgcg tgcacccgtc gcgatgatga cgtgcttcgt gtcgaggacg
     3421 acctggccgc cgccggcttg atccacttgc acctgcagcg gcttccacgg cccgcttcga
     3481 ggcagcagct tcgcggtgcc tgcgatgctc tcgagcttgt tcttcttgaa gaggtacgcg
     3541 acgcccttgc tgatcttgtc ggcgatatcg cggctccgct tgatgatcgc cgtgaagtcg
     3601 ggacgcggat tatcgacgag gatcccgaag tccttcgcgt gctgcgcgtg ctcgaggatc
     3661 tccgcgctct tgagcagcgc cttcgtcggg atgcagcccc agttgaggca gatgccgccg
     3721 agctccgcac gctcgatgca cgcggtcttg agcccgagct gcgcgcactt cacggcagcg
     3781 acatagccgc ccggaccact gccgatcacg atgacgtcga agccgctcat agcgcgagcg
     3841 attccgggtg ctcgatcaga tcgacgaacg cggcgagcca acgtgcgccg agcgctccgt
     3901 cgatgacgcg gtgatcgcac gacatcgtca ccgtcatgcg cttgcctgcg acgacttcgc
     3961 tgcccttgac gaccggcgta tcgacgacgg tgccgattgc gaggatgccg gcctcgggcg
     4021 ggttgatgat cgccgtgaag cgctcgatgt tgtacatgcc gaggttcgac acggaaaacg
     4081 tcgagccaga aatctcgtgg cccttgagcc gcttgttctt cgcgcgatcc gcgaggtcgc
     4141 gaacctccgc cgcgatcgag ccgatgccct tctgatcggc atcgcgaatg accggcgtga
     4201 tgagcccttc atcgagcgcg acggcgacgc cgatgtgcac gcgatggaac ttgcggatgg
     4261 cgttgccgtg ccactgcgcg ttgcactcgg gcacgcggcg cagtgcgagt gcgacggcct
     4321 tgatgatgag gtcgttgacg ctgaccttgc ccttatcgcc gagcagttcg ttgagccggg
     4381 cgcggaacga catcatcggc gccgcgtcga acgtgcgcat caagtagaag tgcgggacat
     4441 cgcgcttggc ctcggtgagc cgtgccgcga tggtcttgcg catgttcgaa gcaggaatgt
     4501 cctcgtagtc gccctcggct tcgggcgtca tctggcgcgc gtgacgaacg gcgagggcct
     4561 gcgtcgtgcg cgactcgatg tcgtcgtctt gctcggcttc ctgcggctcg ccggccttcg
     4621 cctcctgcgc gtggccgttg ctaccgcctt cgccaccttc ggcagcagcc ttcacgtcgc
     4681 gctcgacgat tcgcccgccg ggtcccgagc cctggatcgt ccgcagatcg attccgagct
     4741 cgatcgccaa cgtcttcgcg agaggagagg cgaggagctt gccgccgctt gcctgcgctg
     4801 cgggcttcgc tgccggttgg gccgctttcg gagcaggcgc tttctgtgct ggcgtttctt
     4861 tcttggcggc cggcgcggat gtcgagactt cggggccttt gggaggcggg gccgattgct
     4921 tgggagcttc ttgcttggcc gccggtgccg caccgccgcc gccggacttc gcttggtcga
     4981 cgagcgcgct gacgtcttcg ccggccttgc cgatgatcgc gaccggcgcg ccgagcttca
     5041 ccgtgtcgcc ctctttgacg agcagcttca acaggacgcc ttcgtcctcg atgttgaagt
     5101 ccatgttcgc cttgtccgtc tcgacctccg cgacgagatc accgggcgag accttgtcgc
     5161 cttccttctt cgtccaacgg acgaggacgc cttcttccat cgtcggcgag agcttcggca
     5221 gaccaatgat ttgcgccata aatcaatcca ggtagagaac gcgacgaacg gcagcaacca
     5281 cgtctttgat ctgcggttgc acttcatctt cgaggttctt cgcgtagggc atcggcacgt
     5341 cgtcgctcgt gacacgctcg atcggcgcgt cgagcgaatc catgcagtgc ttctgcatct
     5401 gatacgcgat ctctgcgccg aagcccgcga cgggccagcc gatctcgacg atcacgcagc
     5461 ggccagtctt gcgcacactc tcggcgagcg tgtcgacatc gagcgggcgc agcgtccgcg
     5521 gatcgacgac ctcgcactcg atgccttctt cggcgaggac ttttgccgcg tcgaggcatg
     5581 tccacaccat cttcgaccac gcgatc
//
LOCUS       DATLBZ010000620         8341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8732649, whole genome shotgun sequence.
ACCESSION   DATLBZ010000620 DATLBZ010000000
VERSION     DATLBZ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8341
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8732649"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VMZ53_30470"
     CDS             <1..187
                     /locus_tag="VMZ53_30470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32880.1"
                     /translation="NTSCGGVGNACVTIGSSGRRCVAACTADSGCPTGYQCKAVASTS
                     TSTIYGSYCVPATRSCN"
     gene            complement(184..693)
                     /locus_tag="VMZ53_30475"
     CDS             complement(184..693)
                     /locus_tag="VMZ53_30475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32881.1"
                     /translation="MRQLLVCFALAACTTNNGPMSSGDDDGDDAAPDAGTSTTSVTPV
                     AGLWYYAESSAVHSSCSQPVQGEAGEFGIDMVTKSAFRIVPNDGTLPFSCTLAGAEFD
                     CPDRAWDSEELANLDATLIVHGTAEGTFSSSTRATGQQHATVTCTGSDCGSLGLPCTF
                     DVNFVIRAR"
     gene            complement(761..2251)
                     /locus_tag="VMZ53_30480"
     CDS             complement(761..2251)
                     /locus_tag="VMZ53_30480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HUS32882.1"
                     /translation="MVAVAELSFTDRFVRALPGDPRQDNRTRQVMQACYSRVEPTAVK
                     APELIALVPEVAALLDLDPTPTRELVDVLAGNRVLPGMAPYAACYGGHQFGTWAGQLG
                     DGRAITLAEVQNRAGETWEIQLKGAGLTPYSRHADGRAVLRSSLRELVCSEAMFHLGV
                     PTTRALSLVTTGEPVMRDMLYDGHPAEEPGAVVCRVAPTFLRFGNFEIHSSRDDHATL
                     KKLVDFTLERYFPGRDVAGMFEDIMLRTAWLVAEWMRVGFVHGVMNTDNMSILGLTID
                     YGPYGWLEPFEPDWTPNTTDAGGRRYRFGNQPAVAHWNIAQLARALAPIAGDLDPLRK
                     TIDRFPQEFSTHYRNTMLRKLGLRDEDGAEQLLEQLAELMIATKPDYTLFFRRLVDPT
                     YDSVRDAFYGDHDRAAIDAWLAEYRTRDRDVAGMRAINPIYVPRNYLVQEVIEATEKG
                     DRKAVLDLLDVLRHPYDEQAGREHFAGKRPEWARSKVGCSMLSCSS"
     gene            2275..2781
                     /locus_tag="VMZ53_30485"
     CDS             2275..2781
                     /locus_tag="VMZ53_30485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32883.1"
                     /translation="MICRLAILTVLLAGATAHAEPSWHAGLNIRGDLGTHPIRIGGGL
                     QLGRVDAILVVDPMFWTDGQVDTDLLAFWAPCSRAWGLLGGWRTSTISILGGTEFQEK
                     LIVGVGAPLPQMGHHIRARFHFELATVVVKHGAGLPADWISFATGRDFIDLVNFGMFV
                     SFEYANHL"
     gene            2765..3604
                     /locus_tag="VMZ53_30490"
     CDS             2765..3604
                     /locus_tag="VMZ53_30490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32884.1"
                     /translation="MRIISSALVALALAACDRPQTLLICHNSNCAEPADPENDDTLAA
                     LNESLTLSVEGKPAIDGIEVDLFWRASDDTCIFAHDLETARMDLATQAADAIAFHITT
                     APQLTFGGGNFQMFVELKDFVDAAKTVHHSPEQRMMHAACAWDFYTRVEQAADARGVT
                     IDFFFGSFGPEMLKEVIRQTPPNLDSAHTPRYEAYYGVPKPLDAETRPLGDYAGIPIT
                     LVEFHSQWITDDAHEAVLAQGIEVAFFMFSATVEHFAAIEQYEPAMVNTSEAILMRRW
                     LER"
     gene            complement(3593..4699)
                     /locus_tag="VMZ53_30495"
     CDS             complement(3593..4699)
                     /locus_tag="VMZ53_30495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32885.1"
                     /translation="MTQLVTQDPLLETVVNDRYQVVRVLGEGGMSLVYEVQHTKLKRQ
                     FALKRLLPHLVSNQEAMIRFEREAELLASLRHPNVVDITDWDELPDGSPYMILEFLHG
                     ASVRVRMDRKQLAWEAIGRFGDQAMAALQLAHRIGITHRDLKPENIFISIDDAGEERV
                     KLLDFGVSKMRGLGRTTGQYAMLGTPSYMSPEQAQGLTDLIGPSTDVWAMGTILYEMA
                     TQKVAFTGESLAATVVNITSGRPAPITSFRPDAPPAFVDLIDRATSPDPDRRIMTIDE
                     LRQGLRAALEPVRSRLHTPVAGLPIVASPSPTPFPATAPSQPLGIVAPSAKTTQNVPK
                     TEAAPAPAAKMNYMVIVGTALVAILGVVIGALAF"
     gene            4795..5670
                     /locus_tag="VMZ53_30500"
     CDS             4795..5670
                     /locus_tag="VMZ53_30500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32886.1"
                     /translation="MVRVVILALVAFTATAHAQNAFDVCKGESKPLVEGKGKITIAVV
                     YPEDFPVKTTPAMRNAVGAALAKAEKGTIVPAKDVEAAKKLVDAKKWTDKSDACGYSP
                     SLVAVLGQKHKNLSTAHATVACSDDKTCTLRVDLERHGKPTAERWVRYEARLPKTDLA
                     TIQKTAAKLASKGAPPDAPTKGLAVKELTSGAVTVRSDIDGALELDAAMEANAAFAAC
                     APKKKSTKDIRGYWADWKLNAKGTAMEVFVKPFGGVDPTDETASKCLRDALQKMQMAC
                     PRDGQILKVRTAICL"
     gene            5683..6372
                     /locus_tag="VMZ53_30505"
     CDS             5683..6372
                     /locus_tag="VMZ53_30505"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HUS32887.1"
                     /translation="MRSVLLPAAMFVAMVRIAAADGVADDAAPSNRYGAMSRDDCEAE
                     LNARSISFKRETAKGVAQPVRLQGPLHGVTFRTNQHDAERETTIWEIADCRLVLAMDD
                     FAAILEQHDIVEVRHYSIHRAAPKKWPDDKIGKQHIGGLAIDAAKFIRKDGTYLDVLD
                     HFKGRIGAKTCGPKARKAKTEEAKELREIVCAAVDARLFNVVLTPDHNRAHRNHFHLE
                     VMPGVSWILVR"
     gene            6387..7298
                     /locus_tag="VMZ53_30510"
     CDS             6387..7298
                     /locus_tag="VMZ53_30510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32888.1"
                     /translation="MIRYLALAAVCAACSRTPAPKDPTERALFRDLERDVTVTETTGW
                     GIDRIEIESMLERALDSVCRVDELGRRGLHEWLDAQIAESGGPVDEAWRKRGKRLSNV
                     KGLLVLHRVRMLLERAQESSADCPFWLEPENPFRGRQISEHRWSLTFGGGGIGSAIAQ
                     GDRTDISAGGAGRLLIGRMFANGDGLYLGAEVAGSAQFPKDAMGDRTSLQLGVDLVSL
                     LVYRHTLTNTYFEAEGGWLGHSTEEDWGAIDNGMHVGVSFGGRALRQRFLFPGAALTF
                     AWERLFLPGDDLITLKVGARVAFDWDL"
     gene            7295..7864
                     /locus_tag="VMZ53_30515"
     CDS             7295..7864
                     /locus_tag="VMZ53_30515"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HUS32889.1"
                     /translation="MKKQERCTWATKQEMWSYHDHEWGVPVHDDVKHFEFLTLEGAQA
                     GLSWSTILNKREGYRKAFAGFDPAKVARFTPARIEKLLLDPSIVRNRQKVECAVSNAK
                     LYLKLVEELGSFDTYIWGFVGGKPIVNRWKSSNEIPATSKESDALAKDLKARGFKFVG
                     STVMYAHLQAAGLINDHLVGCWRWREVGR"
     gene            7880..>8341
                     /locus_tag="VMZ53_30520"
     CDS             7880..>8341
                     /locus_tag="VMZ53_30520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32890.1"
                     /translation="MWRALSLLAIAGCSFTHGLFDPGSIDAPSDDATLDAPLDAPPDM
                     MLDAFVPYCDDDDPNLVACYPFENNTNDVSGHNLNATMTNVSFVNGKVGKAMQFGSTS
                     AADVADSTRFDVTAITIEAWINPSQLPTNGGRMGIVDMNGQYGFFVHETGNL"
BASE COUNT         1492 a         2690 c         2730 g         1429 t
ORIGIN      
        1 gaacacgtca tgcggcggcg tcggcaatgc gtgcgtgacc atcggctcga gcggtcgccg
       61 ttgcgtggcg gcgtgcaccg cggacagcgg ttgtccaacg ggctaccaat gcaaggccgt
      121 cgcgtcgacg tcgacgagca cgatctacgg ctcgtactgc gtgccggcga cccgctcttg
      181 taattagcgc gcgcggatca cgaagttcac gtcgaacgtg cacggcaggc caagcgaccc
      241 gcagtcgctg ccggtgcacg tgacggtcgc gtgctgctgg ccagtcgcgc gcgtcgagct
      301 cgagaacgtt ccttcggcgg tgccgtgaac gatgagcgtc gcgtcgagat tagcgagctc
      361 ctcgctatcc cacgcacggt ccgggcagtc gaactccgcg ccggcgagcg tgcacgaaaa
      421 cggcagcgtg ccgtcgttgg ggacaatgcg gaacgctgac ttcgtcacca tatcgatccc
      481 gaattcgccg gcctcgccct gcacgggctg gctgcaactc gagtgcaccg cgctcgactc
      541 ggcgtagtac caaaggccgg caaccggcgt gacgctcgtg gtcgatgtac ccgcgtcggg
      601 tgcagcatca tcaccatcgt catcgccgga cgacatcgga ccgttgttcg tggtgcaggc
      661 tgcgagcgcg aagcaaacga gtagttggcg catggttccc ctcttggacg gcatgcccgt
      721 tgtacgggct cgtcggggaa tggccgatgt cagaccgaca tcaggagctg caactcagca
      781 tggagcaacc caccttcgag cgtgcccact ccgggcgctt gcccgcgaag tgctcgcgac
      841 cggcctgctc gtcgtacgga tggcgcagta cgtcgaggag atcgagcacc gctttgcgat
      901 cgcccttctc ggtcgcctcg atcacctctt gcacgaggta attgcgcggc acgtagatcg
      961 gattgatcgc gcgcatgccg gcgacgtcgc gatcgcgcgt tcgatactcc gcgagccacg
     1021 cgtcgatcgc cgcgcgatcg tgatcgccat agaaggcatc gcgtacagag tcgtaggtcg
     1081 ggtcgacaag ccggcggaag aacagcgtgt agtccggctt cgtcgcgatc atgagctcgg
     1141 cgagctgctc gagcagctgc tcggcgccgt cctcgtcgcg caggccgagc ttgcgcagca
     1201 tcgtgttgcg atagtgcgtc gagaattcct gcgggaagcg atcgatcgtc ttgcgcagcg
     1261 gatcgaggtc cccggcgatc ggcgcgagcg cacgagcgag ctgcgcgatg ttccagtgcg
     1321 cgaccgccgg ctggttgccg aagcgatagc ggcgtccccc tgcatcggtc gtattcggcg
     1381 tccaatcggg ctcgaacggc tcgagccaac cgtatggccc gtagtcgatc gtcagcccga
     1441 ggatcgacat gttgtcggta ttcatcacgc cgtgcacgaa gccgacgcgc atccactcgg
     1501 cgacgagcca cgccgtgcgc agcatgatgt cttcgaacat cccagcgaca tcgcggcccg
     1561 ggaagtagcg ctcgagtgtg aagtcgacga gcttcttcag cgtcgcgtga tcgtcgcgcg
     1621 agctgtggat ctcgaagtta ccgaaccgca gaaacgtcgg cgcaacccgg cacaccaccg
     1681 cgcccggctc ctccgccggg tggccgtcgt acagcatgtc gcgcatcacc ggctcgccgg
     1741 tcgtcacgag cgagagtgca cgcgtcgtcg gtacgccgag gtgaaacatc gcttcgctgc
     1801 acacgagctc gcgcaacgac gaccgcagca ccgcgcgacc atccgcgtga cgcgagtacg
     1861 gcgtgaggcc ggcgcccttg agctggatct cccaggtctc gcccgctcgg ttctgcactt
     1921 cggcgagcgt gatcgcgcga ccatcgccga gctgccctgc ccacgtgccg aactgatggc
     1981 cgccgtagca tgccgcatac ggagccatgc ccggcagcac gcggttaccg gcgagcacgt
     2041 cgacgagctc tcgcgtcggc gtgggatcga gatcgaggag cgccgccacc tcggggacga
     2101 gcgcgatgag ctccggcgct ttgaccgcgg tcggctcgac acgcgagtag caggcctgca
     2161 tcacctgccg cgtgcggttg tcctgccgcg gatcgcccgg aagcgcgcgc acgaaccgat
     2221 ccgtaaagct cagctcggcg acagctacca cgccttcatg atacccctgt cgcgatgatc
     2281 tgcagactgg ccatcctgac agtcctgctc gccggcgcta ccgcgcacgc ggaaccgagc
     2341 tggcacgcag gcctcaacat ccgcggggat ctaggaacgc atccgatccg catcggcggt
     2401 gggctgcagc tcgggcgcgt cgacgcgatt ctcgtcgtcg atccgatgtt ctggaccgac
     2461 ggtcaggtcg ataccgatct gctcgcgttt tgggccccgt gctcgagagc gtggggtctg
     2521 ctcggcggtt ggcgcacgag cacgatctcg atcctcggcg gcacggaatt ccaagaaaag
     2581 ctgatcgtcg gcgtcggcgc accgctgccg cagatggggc atcacattcg cgcgcgcttt
     2641 cacttcgagc tcgcgaccgt cgtcgtcaag catggcgcag ggctgccagc cgactggatc
     2701 tcgttcgcga cgggccgcga cttcatcgac ctcgtcaact tcggcatgtt cgtgagcttc
     2761 gagtatgcga atcatctcta gcgctctcgt cgccctcgca ctcgccgcgt gcgatcgccc
     2821 gcagacgttg ctcatctgcc acaactcgaa ctgcgcagag cccgccgatc ccgagaacga
     2881 cgacactctc gcggcgctga acgaatcgct cacgctcagt gtggagggca agcccgcgat
     2941 cgacggcatc gaggtcgacc tcttctggcg cgcgagcgac gacacctgca tcttcgcgca
     3001 cgatctcgag accgcgcgca tggacctcgc gacgcaagcc gccgacgcga tcgcgtttca
     3061 catcacgacc gcgccgcagc tcacgttcgg tggcggcaac ttccagatgt tcgtcgagct
     3121 caaggacttc gtcgacgcgg cgaagaccgt ccatcactcg cccgagcaac gcatgatgca
     3181 cgccgcatgc gcgtgggact tctacacgcg ggtcgagcag gcagcggacg cgcgcggagt
     3241 gaccatcgat ttcttcttcg gctcgttcgg tcccgagatg ctgaaagagg tgatccggca
     3301 aacgccgccg aacctcgatt ccgcgcacac gccgcgctac gaggcctact acggcgtgcc
     3361 gaaacctctc gacgcggaga cgcggccgct cggcgattac gccgggatcc cgatcacgct
     3421 cgtcgagttc cattcgcagt ggatcaccga tgacgcgcac gaagcggtgc tcgcgcaggg
     3481 catcgaggtc gcgttcttca tgttctcggc gaccgtcgag cacttcgcgg cgatcgagca
     3541 gtacgagccc gcgatggtga ataccagcga agcgatcctg atgcgccgct ggctagaacg
     3601 ctagcgcgcc gatcacgacg ccgaggatcg caacgagcgc ggtgccgacg atcaccatgt
     3661 agttcatctt cgctgcgggc gcaggcgcgg cttcggtctt cggcacgttc tgcgtcgtct
     3721 tcgcggatgg cgcgacgatg cctagaggtt ggctcggcgc ggtcgccggg aacggcgtgg
     3781 gtgacggcga cgcgacgatc ggcaggccgg ccaccggcgt atgcagacgc gaccgcactg
     3841 gctcgagcgc ggcgcgcagt ccctggcgaa gctcgtcgat cgtcatgatg cggcggtccg
     3901 gatccggcga cgtcgcacgg tcgatgaggt cgacgaacgc cggtggtgcg tcgggccgaa
     3961 acgatgtgat cggcgccggc cggccactcg tgatgttgac gacggttgcc gccaagctct
     4021 cgcccgtgaa cgcgaccttc tgtgtcgcca tctcgtagag gatcgttccc atcgcccaca
     4081 cgtccgtcga cggcccgatg agatcggtga ggccctgcgc ctgctccggc gacatatacg
     4141 atggcgtacc gagcatcgcg tactgccccg tcgtgcgccc gaggccgcgc atcttcgaca
     4201 cgccgaagtc gagcagcttc acgcgctcct cgccggcatc gtcgatcgag atgaagatgt
     4261 tctcgggctt gagatcgcga tgcgtgatgc cgatgcggtg cgcgagctgc agcgcagcca
     4321 tcgcttgatc gccaaaccgc ccgatcgctt cccacgccag ctgcttgcga tccatgcgca
     4381 cgcgcacgct cgcaccatgc aagaactcga ggatcatgta cggcgagcca tcgggcagct
     4441 cgtcccagtc cgtgatgtcg accacattgg gatggcgcag ggacgcgagc agctcggctt
     4501 cgcgctcgaa ccggatcatc gcctcttggt tgctgacgag gtgaggcagc aagcgcttga
     4561 gcgcaaactg ccgcttgagc ttcgtgtgct gcacctcgta gacgaggctc atgccacctt
     4621 cgccgaggac ccgaacgacc tggtagcgat cgttgacgac cgtctcgagc aacgggtcct
     4681 gcgtcaccaa ctgcgtcact acagtcatag taccgaacgt gcagcggcat ttcctgcgcc
     4741 tatgtaggtg acgctgctcc agcgaagcgc cgcggttccc gttatcattg aggcatggtt
     4801 cgcgtcgtta tcctcgcgct cgtcgccttt actgccaccg ctcacgcgca gaacgccttc
     4861 gatgtctgca aaggcgagag caaaccgctc gtcgagggca agggcaagat cacgatcgcc
     4921 gtcgtctatc cggaagactt tccggtgaag accacaccgg cgatgcgcaa cgcagtcggt
     4981 gcggcgctcg cgaaagccga gaagggcacg atcgtcccgg cgaaggatgt cgaagccgcc
     5041 aagaagctcg tcgatgcgaa gaagtggacc gacaagagcg acgcatgtgg ctactcgccg
     5101 tcgctcgttg cggtgctcgg acagaagcac aagaacctct cgacggcgca cgcgacggtc
     5161 gcctgcagcg acgacaagac gtgcacgctg cgcgtcgacc tcgagcgtca cggcaagccg
     5221 accgccgagc ggtgggttcg ctacgaagcg cggctcccca agacggatct cgcgacgatt
     5281 cagaagactg ctgccaagct cgcaagcaag ggagcgccgc cggacgcgcc gacgaaaggc
     5341 ctcgcggtga aggagctcac gtcgggcgcg gtcacggttc gcagcgacat cgacggtgcg
     5401 ctcgagctcg acgctgcgat ggaagccaac gctgcgttcg cggcgtgtgc gccaaagaag
     5461 aagtcgacga aggacatccg cggctactgg gcggactgga agctcaatgc gaagggcacc
     5521 gcgatggaag tgttcgtgaa gccgttcggc ggcgtcgatc cgacggacga gaccgcatcc
     5581 aaatgcctgc gcgacgccct ccagaaaatg cagatggcct gtccgcgcga cggacagatt
     5641 ctgaaggtcc gtaccgcgat ctgtttgtag tagggtcgcc gcatgcggtc ggtcctgctc
     5701 cccgcggcaa tgttcgtcgc gatggttcgc atcgccgctg ccgatggagt tgccgacgac
     5761 gccgcgccgt cgaatcgcta tggtgcgatg agccgggacg actgcgaagc ggagctcaac
     5821 gcgcgcagca tctcgttcaa gcgcgagacc gcaaagggcg tggctcagcc ggtgcgcctg
     5881 caagggccgc tgcacggcgt gacattccgg acgaaccaac acgatgcaga acgcgagacg
     5941 acgatctggg agattgcgga ttgtcgcctc gtgctcgcga tggatgactt cgccgcgatc
     6001 ctcgagcagc acgacatcgt cgaggtgcgc cactactcga tccatcgcgc ggcgccgaag
     6061 aagtggcccg acgacaagat cggcaagcag cacatcggcg gtctcgcgat cgacgcggcg
     6121 aagtttatcc gcaaggacgg cacctacctc gacgtgctcg atcacttcaa aggacgcatc
     6181 ggtgcgaaga cctgcggtcc gaaagcgcgc aaggcgaaga ccgaggaggc gaaggagctg
     6241 cgcgagatcg tgtgtgcggc cgtcgacgcg cgcctgttca acgtcgtgct cacgccggat
     6301 cacaatcgtg cgcatcgcaa tcacttccac ctcgaagtga tgcccggcgt gtcgtggatc
     6361 ctcgtccgct aggttacgct cgtcgagtga ttcgctacct cgcgctcgcg gctgtctgcg
     6421 cggcatgttc gcgaacgcct gcgccgaagg atccgacgga gcgcgcgctg ttccgcgatc
     6481 tcgagcgcga tgtcacggtg acggagacga cgggttgggg catcgaccgg atcgagatcg
     6541 agagcatgct cgagcgcgca ctcgactccg tctgtcgcgt cgacgagctc ggccgccgtg
     6601 ggctgcacga atggctcgat gcgcagatcg ccgagagcgg tggtcccgtc gacgaagcgt
     6661 ggcgcaagcg cggcaagagg ctgtcgaacg tgaaggggct gctcgtcctg catcgcgtgc
     6721 gcatgctgct cgagcgcgcg caggaatcgt ccgcagattg tccgttctgg ctcgagccgg
     6781 agaacccgtt tcgcggccgt cagatctccg agcatcgctg gtcgctgacg ttcggcggcg
     6841 gcggtatcgg cagcgcgatc gcgcaaggcg accgcacgga catcagcgcc ggcggcgcgg
     6901 gacgcctgct gattggccgc atgttcgcga acggagatgg cctgtatctc ggcgccgagg
     6961 tcgcgggcag cgcgcagttt ccgaaggacg cgatgggcga tcgcacgtcg ttgcagctcg
     7021 gcgtcgatct cgtttcgctg ctcgtgtatc gccacacgct gacgaacacc tacttcgaag
     7081 ccgagggcgg ctggctcggt cactcgacgg aagaggactg gggcgcgatc gataacggca
     7141 tgcacgtcgg cgtgtcattc ggtggccgcg cgctgcgcca acgctttctg tttcccggcg
     7201 ctgcgctgac gttcgcatgg gagcggctgt ttcttccggg cgatgatctg atcacgctga
     7261 aggtcggcgc ccgcgtcgcc ttcgactggg atctatgaag aagcaagagc gctgtacgtg
     7321 ggccacgaaa caagagatgt ggagctatca cgatcatgaa tggggcgtgc ccgttcacga
     7381 cgacgtgaag cacttcgagt tcctcacgct cgagggtgcg caagccggcc tctcgtggtc
     7441 gacgatcctc aacaagcgag agggctatcg caaagcgttc gcgggcttcg atccggcgaa
     7501 ggtcgcgcgc ttcacgccgg cacggatcga gaagctgttg ctggatccga gcatcgtccg
     7561 caatcggcag aaggtggagt gtgcggtgtc gaacgcgaag ctgtatctga agctcgtcga
     7621 ggagctcggc agcttcgata cgtatatctg gggcttcgtc ggcggcaagc cgatcgtcaa
     7681 ccggtggaaa tcgagcaacg agattcccgc aacctcgaag gagtccgatg cgctcgcgaa
     7741 ggacctcaag gcgcgcggct tcaaattcgt aggctcgacg gtgatgtacg cgcacttgca
     7801 ggccgcgggt ctcatcaacg atcacctcgt cggctgctgg cgctggcgag aagtcggtcg
     7861 ctgatcctac aatcgacgga tgtggcgggc gctctcattg ctcgcgatcg caggctgcag
     7921 cttcacgcac ggcctgttcg atccgggctc gatcgatgcg ccgtccgacg acgcaacgct
     7981 cgatgcgcca ctcgatgcgc cgccggacat gatgctcgat gcgttcgtcc cgtactgcga
     8041 tgacgacgat ccaaatctcg tcgcctgcta tccgttcgag aacaacacca atgatgtctc
     8101 gggccacaac ctgaacgcga cgatgacgaa cgtgtcgttc gtaaacggca aggtcggcaa
     8161 ggcgatgcag ttcggctcga cgagcgccgc cgacgtcgcg gacagcacgc gctttgatgt
     8221 caccgcgatc acgatcgaag cgtggatcaa tccgtcgcaa ctgccgacga acggtggccg
     8281 tatgggcatc gtggacatga acggccagta cggcttcttc gtgcacgaga ccgggaatct
     8341 c
//
LOCUS       DATLBZ010000621        18078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3531729, whole genome shotgun sequence.
ACCESSION   DATLBZ010000621 DATLBZ010000000
VERSION     DATLBZ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18078
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3531729"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2294
                     /locus_tag="VMZ53_30525"
     CDS             <1..2294
                     /locus_tag="VMZ53_30525"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HUS32891.1"
                     /translation="ADFNEYRWVFLSPYGRWGFYVGLVIVSAIVLLAWRASRGSSTWR
                     RATLIGLRGAAAVGALVVLLEPAVELRQVAREPNRIAVLIDDSKSMSLAESPQGPTRI
                     ERVRRLIRDSQETLAAWQREHKIDYYTFSETVSSTSPLALEADPAQGKSTLIRKALEY
                     VRSRYEGRDLAGIVLISDGAATGSFDEDSGDGAVRDFLRSLDTRVHTVWAAREGLRDV
                     AVAKVNADEFAFVRTATKIDAIIRTTGMPARRIPVTLSLDGQPVRQKMVDLPAGEQEV
                     TVSFEVMPLRVGRYVYEISVPVAAGEAVTTNNTRNFVIRIIRDKIRVLQVAGAPSWDV
                     RALRQWLKSDPNVQLISFFILRTQDDIQMAQNDEMSLIPFPTRELFEEQLPSFDVIVL
                     QNFEYLPYGLNEYLENIRSFVDGGGGLVMLGGAQSFTSGGYFGTPVAAALPVDLYGPF
                     DSGPVLDTQKFSPQLTSAGEMHPVTSLRYSATDNVAAWKALPQLEGVNLIAGAKSDAT
                     VLATHPRLKTKHGKPMPVVVAGEYGKGRSVIYTTDTLWRFGFVAAARPGDRGQAYAKL
                     WENTIHWLIQDPDLRNLHVDSDAVEYVPGQAVKVTVRLLGRDYQPLPNGVASLVVKRG
                     ADPNAAEQVQQTKLTVGEDGTATYELGGLTPGVYRVLGSATVAGRQVEASDIFLVREG
                     GSELDRPVGDRDMLRSIADATGGSALGPIDELPSHLKLDPPRVVRVDRRTDIELWSRP
                     GLLILIVGLLGLEWLLRQRSGYL"
     gene            2309..3556
                     /locus_tag="VMZ53_30530"
     CDS             2309..3556
                     /locus_tag="VMZ53_30530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32892.1"
                     /translation="MLQLDSIVTDLMFQSTASIDQQTLIEGLGGETVLQTKQLLDVDR
                     LDPRAEMELSPSMPFRRRGERRLMIIDDQATTPREDPQNDPVIKTDLRPFKEALIASA
                     QKLPLRLGRIFALGAWMDAVIVARSVRDLRGITLLSWALDPHVDIDGKRRQTPLSQKE
                     FEAKLIAYEKRLDELDEQQILDRLTGLATFERRGDLVVVDVLEPDGTWDQRKSMLLEQ
                     QLAAWDTFSMIPGAPSQSAKKPGAPAPSNGEPAKAAAKSEPAKAAAPAKTAAPAKPPE
                     PVKEEPKGPPIQAQEIDGKIVLVFPPERFDLDVAAALGKRDWDNVVRRSDNMTGAMRD
                     KMHRDGACWIAPLEFLSEVFIDGKPLTKQQFEKDAQPVDGARALEVHFPRFGEVILLD
                     VPGKGRYVTSLTDRAERALSLVK"
     gene            3557..4705
                     /locus_tag="VMZ53_30535"
     CDS             3557..4705
                     /locus_tag="VMZ53_30535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32893.1"
                     /translation="MLRVVVVLAALMSVARADDDLGQRTVVFARGTTLYKSDAKGKNE
                     TELATLPAKSTVRALRTDALGKILLADIGGTWSWMPLDGSTTSLTDLPCDAGPAQLAE
                     DGLCVLCRQRKQGSTGGSIIVNLATNKVTPVDVPTTGARLAGMGAERKLVWADDKGVW
                     TASPATPKKATKAAPEAPLRNLSPSPDGAHALGTFNGEVFESPKVKKPGEILMEFALD
                     GDGARRKLIQNGVPVEWSHDSKWILLQDGSSACIVQAAGGQYKCWKGYTAAALAPDGK
                     YALLLGNRDGSKKQATKDDKKKSSKSSKKNSKKKKEPDPPPEPETDEGADVEGEGGDD
                     EIPTDDVAVAPPSGPLALYIAQLDGAFTKSPKLLSKVVDGAAVFVPAP"
     gene            complement(4702..4959)
                     /locus_tag="VMZ53_30540"
     CDS             complement(4702..4959)
                     /locus_tag="VMZ53_30540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32894.1"
                     /translation="MTSQATSSSTQLARGSSREVAAVSKPRARVDTPVSAFQLDETTR
                     QSQLEMTLRRPKTDNSRIYMIAIIALLACTVAVLAVLLLVF"
     gene            complement(5112..5645)
                     /locus_tag="VMZ53_30545"
     CDS             complement(5112..5645)
                     /locus_tag="VMZ53_30545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32895.1"
                     /translation="MRRILGVLAICACGGASPPPAKPMAPTASKPSPVPREAAVTPAP
                     KEQEPVILHAKAEMTPTKGSKLEPFVIAFMQRQGDNALVLADLPVGVKAGTYHYVIHE
                     GSECAKPGPTWAETKDVDLRLVVLEDLTGALDNTELAAHLEGEQTLIGHTLVLHDDKK
                     GKPGKALACAPIVASDE"
     gene            5711..6190
                     /locus_tag="VMZ53_30550"
     CDS             5711..6190
                     /locus_tag="VMZ53_30550"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HUS32896.1"
                     /translation="MLVVIHSGETGVERGAHYGARTVELAVNGFMPLQGRDELGKIPV
                     DVAAYLVPHAARSRREAQIANIELSSAVLLLVPEAEKASAFPATRWLLTEIRNLGRRH
                     FVADPKSSTDAVAEWLWAMAKAPEPLRLFITGPRGTRWDRGETFARRLVQTLAMEKP"
     gene            complement(6187..6783)
                     /locus_tag="VMZ53_30555"
     CDS             complement(6187..6783)
                     /locus_tag="VMZ53_30555"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HUS32897.1"
                     /translation="MRTALVLIALLVACGPPVPPVGSVAKFWTVDPRGQEVVLGAGDV
                     LEISVWNQKDLDTETVVRPDGTITMPLVGDVKAAGQTPKVVHDKITTALQNFIKIASP
                     NEVTITVKSYMSYRFTVQGEVGKPGQYSSPSYLTVSDAIALAGGPTRFAKRDSMVLFR
                     KDSKGESQSLNISYDLIASGKRPDLNVWILADDVLYMP"
     gene            complement(6855..9740)
                     /locus_tag="VMZ53_30560"
     CDS             complement(6855..9740)
                     /locus_tag="VMZ53_30560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32898.1"
                     /translation="MRLPVVCLSIAAAALVIVTPRLVEAGCSIPGFNKAAFGDIGVDI
                     GNGQSDSYNSASGSYASTKCSTATFPNSCLGAVGTNAITNSAVSVGPNGKVNGDCQVG
                     VGGSTATAIGGSGGSSKCGSTSSQSSAITLSSVTAPTWPNGSIGACTGSPTACSVTSA
                     PVTLTANKTYGGVSLSGNATEVLTVSAGDYVFTGNWSIGGNASVKKAGTGTAYIYVTG
                     TSVSIAGNGISNTDASGNIGKPTDLIFMCTDSVTSATVTGGGNGAFAFYCPKADITVN
                     GNGAVYGAIVGKKVTFNGNNAIVHYDAALASLTSSQLACQYETARGAPVIASLTPSYG
                     GSAVDVLVQGSFESPGTKTSISSSGASGTGTVAAFAFPYVKGHVRARLVSTIGATASS
                     ITGNTVCTASGVPSGCNTLFDAGANVPAPNATCSTPDGTCRYVFTNLTSSATGRVFHP
                     TKTQLAYNDTNTTRIDSIGALMVPSTAVAGMTTPLRKSLINLVLDSKAGGTTRTIGGV
                     DRSSVAVVGPSSNGAGSASRATIVYWGGLDGMLHAACAQKTTSGLCSASGVTAGTELW
                     AFLPRVQLPYIRTNDQRIDGSIRVMDMYGDFTNDPATGSRSWKTVLIFQTGYAINGTP
                     AVYALDVTDPSTPTILWEVTPPSSLGSTEIGAGLRVVMGAVRVGGRLQNLAVVETRNG
                     GSGGAGVVVKALSVETGAEIWKFSSLYPTTLSPSATGIPGGAVGVDIVGTGEYSDFVF
                     GDLYGRLWMVNSQTGVSRTGATTPLFAFGTNATTKRPIGQPPAIYSNGSSQFAVVSSG
                     AYVDHTDTTGTGLWSSGTQYVVGVKLNQAATSSASESTTACATCAVSFATSFTGFGFA
                     QAIVVGTQLFVTTDSQDANGSSYGATANTGSLRTYALGTTAIAPALSTTTPIAAGATS
                     LAQSGTTLVASGGQKQQKMTSATTTAGNKVDLSAFSKITRMLWLRAE"
     gene            complement(9822..10346)
                     /locus_tag="VMZ53_30565"
     CDS             complement(9822..10346)
                     /locus_tag="VMZ53_30565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32899.1"
                     /translation="MTKRNPERGSAMIVTLIIIASMLAGAAVLVSMQLAGNRSTDLSR
                     TGMTSLHCAEAGLASSRALVKANFAQWGTAITASAGGNTNLPSFLSTIDRDIDNDGSD
                     DFTVYLKDNDDEGAPTANDLTKDQDLRVFVISKCTKFPDNPKQVMELVQYTGGNSCDY
                     KEDGGCDGNGSFAQ"
     gene            complement(10343..11326)
                     /locus_tag="VMZ53_30570"
     CDS             complement(10343..11326)
                     /locus_tag="VMZ53_30570"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HUS32900.1"
                     /translation="MKQETTQRGFTLIELMISLVLFSFAVAGLLSIGVAVTQGYAEQR
                     RALAAESAVRVPLDFLADALRQASPGVSTPANLQDSGTCNTGAINVIDSSSGSDELYL
                     MYAAGAVVTSTREIYDNTSQNIDVTNASQFADDDAVIISNFTNGTLLHVDTVDTSANT
                     LTFASPCSGLTVPSAGSVAGYPVGSLVIRAQYAHFYIADLDGIPTLWMDPDAAGTAFQ
                     PEPLAEGIEDMQIAVGIDGDSDGSISENGLGAGDDEWWFNFAGEAMPSTGSIRAVRIT
                     LVARATKPLVGNAPLYYRPGAENRIGSTAPDAYRRRVLRTIVDMRNIGGSP"
     gene            complement(11323..11859)
                     /locus_tag="VMZ53_30575"
     CDS             complement(11323..11859)
                     /locus_tag="VMZ53_30575"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUS32901.1"
                     /translation="MGRAAAARSTSARRKVLASIECSSTKRRGAPMHARRGSEHGFTM
                     IEVMIAILLSAIAVIGIIGLFVAQGRAAGRSRHETEASALANDKLEKLRTMATPTGSS
                     ETMIDVQGVTGNTSSIFERKWTVSSSGGVTTLEVKVGWDDDDVPTQTCATDADCSSKF
                     CKVTTCMSYAVSVYGMRL"
     gene            complement(11747..12265)
                     /locus_tag="VMZ53_30580"
     CDS             complement(11747..12265)
                     /locus_tag="VMZ53_30580"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HUS32902.1"
                     /translation="MMRARLQRGFTLIELMVVIAIIAILATLLISLSGRTYGANAYNV
                     SDKVVSTVGWVRMRAVSTRRIQTLQIKNQEMFIWQSPITGFATTSATPEFVGRVSIPN
                     GVTIYQVDGQPETSTGNSVALTANLDVSITFKPDGSSSGGTIYIGETQGARQYRVLIY
                     KATGSAYARETW"
     gene            complement(12262..12813)
                     /locus_tag="VMZ53_30585"
     CDS             complement(12262..12813)
                     /locus_tag="VMZ53_30585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HUS32903.1"
                     /translation="MRETTAERGFTLIELMMVVAIIAVLAIIVVPTFVGEARKAKGQA
                     EVNAMFTAISTKQETYKNEFNSYLALPMCPTTGPSVAGANIGKMIVDGDCDAAWVTAR
                     VAAPESKMHCAYTVVVGAKGTTLTPPTGFKNSQGVAAAEPTLASGWWYATAECDEDGQ
                     GGTNAMYYESSVDTNIQVTNAAK"
     gene            complement(12873..14555)
                     /locus_tag="VMZ53_30590"
     CDS             complement(12873..14555)
                     /locus_tag="VMZ53_30590"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS32904.1"
                     /translation="MKKLMAIALLAACRTVPNPPITPAPAPAPEPTPAPAPQTKPAET
                     PMPAITQTAQPQTLQFPDEDFRRTQPQGTAPHDFKLPAVKPFKLKNGIQVYLVEQHNL
                     PIVSMDLNFDGGSITDPKGKDGLAGACMSLLTEGTQKLDKIQYSEALADVASNINAYA
                     GDDTQGVQLASLTKHLDTTFALFADTILTPGMRESDFDRLKKRRIEAVRQSRTSPTSI
                     PGRVTGAILFGPEHPLGSVLTEDEVKAVTLDDCKAYVSKWLKPKNARLFVVGDLTEEQ
                     LRAQFEKSPLGAWTGAGPKLAALPKPKTMKGKIFFVHVPNAAQSNVMMLSFGPTRTAP
                     DYFANSMMGAVFGGSFTSRLNMNLREDKGYSYGARGGFGYSPKQFGQLTVNAPVQADS
                     SYQALREVHRELVELASGKRPVTDEELDREKTNAILALPGRFATAQAALGQYRSLVYY
                     GLPLDYFNTYVEKVKKVTAAQAKQSATKHLKEKDAVFLVVGDGDAKMIVDDPKAGALP
                     KDRKKPYEVDGRQLTLREALQKLAEKGDVGAGGLVELDVDGNPITPANAKSK"
     gene            complement(14552..15883)
                     /locus_tag="VMZ53_30595"
     CDS             complement(14552..15883)
                     /locus_tag="VMZ53_30595"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUS32905.1"
                     /translation="MRPLLIAAVLAASVSTAHADDPKLEFEKYKLPNGMEVILSPDNR
                     VPLVAVNVWYHVGSGNEVPGKSGFAHLFEHMMFQGSKNVGSDKHFEILRKIGSAEVNG
                     TTNPDRTNYYEVVPSHFLETAIWLESDRMGHLLELVDRKALDNQIDVVRNERRQNYDN
                     VPYRKALFALYAAMYPEGHPYRYLTIGKHEDLTSASLEDVKGFFKTWYVPANATLTIV
                     GDFDKAEAKKLVEKWFGTYPASEKPKWVKVPAPAQKATSVSVDDTFAKQRQVVFAWHS
                     PAAYVAGDAELDIVANALGAEGRGRLYKALVLDNQLATRVSVSQDGSTFSGTFDVTVT
                     LRTEADMEQVKKIVADEVAKVTKENLSAKEIERVVTASEASMIYRLEGLNARANVLQT
                     YNQFLGDPGKLSWDLDRYRKATPDGIRAAAAKYLVPTSMVTITTNPAGGSK"
     gene            complement(15899..17554)
                     /locus_tag="VMZ53_30600"
     CDS             complement(15899..17554)
                     /locus_tag="VMZ53_30600"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32906.1"
                     /translation="MADHRNPSGRRPPVLPLPAPPPEEDSSRIRRGHDDDLPRLPEVN
                     VDDSSEVRIDHRALAPGRDSLSGAREIPRHRHDTTRVGRPATQERTPNTSKVRGGDAT
                     FGRGREGTRPPAAGMHTPVAPPRSAMVRVTTAQHSSLIGGRYQIVKRIGQGGMGKVYE
                     VNHIHLSRRFALKIISNQVAETDEARELFYREARFASAMSHPNITSVVDFGEDEKVGM
                     FMVMEFVGGEPLHRILFREKRLSVKKASEIVLQVAEALHYIHKQNVVHCDIKTENILI
                     QEEEIEGKRSKMVAKLLDFGLARPLTGPRASTSLSGTPHYVAPERIRGEPASPSSDVY
                     GVGILLYELLTGQVPWDGAVQKILQGHLDEKPRPPSSLIEGGLDPAVENLVLHALAKR
                     PAERHKDMAAFIYELKTVMDMLGIGRRRGVGAAPARRVVIEQKPPETNKRDEFARVAF
                     DACRLPLALLSPQGLIVAANPAFAKFVMGISVEVEGLPVKSTPLAQAWSTAEQDIART
                     LAGSPIRRIIEIDVSADETRRLLLWLDAVSRDHVVLGVQPLEL"
     gene            17589..>18078
                     /locus_tag="VMZ53_30605"
     CDS             17589..>18078
                     /locus_tag="VMZ53_30605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32907.1"
                     /translation="MQELWSSESYEPHLLSLPFAFAPVAMLVVIAYALVMRGEPKLRA
                     WMLLHFFALLPYALTMTLSPSLVSQEVADHLFRIAGACIPMAAAAGCGFQYQLIGQAH
                     RRRWWVWGGVVVALAWLVIGTTTDLIANGVRWLPAKFWYANAGSLTWLILLTTVINAT
                     PAF"
BASE COUNT         3056 a         5825 c         5893 g         3304 t
ORIGIN      
        1 ccgcggactt caacgagtac cgctgggtgt tcctctcgcc gtacggtcgc tggggctttt
       61 atgtcggcct cgtgatcgtg agcgccatcg tgctgctcgc gtggcgtgca agccgcggct
      121 cgtcgacctg gcgccgcgca acgctgatcg gtcttcgcgg tgcagccgcc gtcggtgcgc
      181 tcgtcgtcct gctcgagccc gccgtcgagc ttcgccaagt cgcacgcgag ccgaaccgca
      241 tcgccgtcct gatcgatgac agcaagtcga tgtcgctcgc cgagagcccg caaggaccga
      301 cgcgcatcga acgcgtgcgc cgcctcattc gcgattcgca agagacgctc gccgcgtggc
      361 aacgcgagca caagatcgac tactacacgt tctccgagac ggtcagctcg acgtcgccgc
      421 tcgctctcga ggccgatcca gcgcaaggca agtcgacgct catccgcaaa gcgctcgagt
      481 acgtgcgcag ccgctatgaa ggccgcgacc ttgccggcat cgtgctcatc tccgacggcg
      541 cggcgaccgg cagcttcgac gaggattctg gcgacggcgc cgtgcgcgac ttcctccgct
      601 cgctcgatac gcgcgttcac acggtgtggg ctgcgcgcga aggcctgcgc gatgtcgcgg
      661 tcgccaaggt caacgccgac gagttcgcgt tcgtgcgcac cgcgacgaag atcgacgcga
      721 tcatccgtac gacgggcatg ccggctcgtc gcatcccagt gacgctctcg ctcgatggcc
      781 agcccgtccg ccagaagatg gtggacctcc cggcgggcga acaagaggtg acggtcagct
      841 tcgaagtgat gccgctgcgc gttggccgct acgtctacga gatcagcgtg ccggttgctg
      901 ccggcgaagc ggtcacgacg aacaacacgc gcaactttgt tatccgcatc attcgcgaca
      961 agattcgcgt gctgcaagtc gccggtgcgc cctcatggga tgtgcgcgcg ctgcgccagt
     1021 ggctcaagag cgatccgaac gtccagctga tctcgttctt catcttgcgc acgcaagacg
     1081 acatccagat ggcacagaac gacgagatgt cgctgattcc gtttccgacg cgcgagctgt
     1141 tcgaggagca gctaccgtcg ttcgacgtga tcgtgctgca gaacttcgag tacctgccct
     1201 acggtctcaa cgaatacctc gagaacatcc gcagcttcgt cgacggcggt ggtggtctcg
     1261 tcatgctcgg tggcgcgcag tcgttcacgt cgggcggcta cttcggaacg ccggtcgcgg
     1321 ccgcgctgcc ggtcgatctc tacggtccgt tcgactcggg tcccgtgctc gacacgcaaa
     1381 agttctcgcc gcagctgacg tcagcgggag agatgcatcc ggtcacgtcg ctgcgctact
     1441 cggcgaccga caacgtcgct gcatggaaag cgttgccgca gctcgaaggc gtgaacctca
     1501 tcgcgggtgc gaagtccgac gcgacggtgc tcgccacgca tccgcgtctg aagacgaagc
     1561 atggcaagcc gatgcccgtg gtcgtcgccg gcgagtacgg caaaggccgc tcggtgatct
     1621 atacgaccga tacgctctgg cgctttggct tcgtcgctgc agcgcgaccc ggcgatcgcg
     1681 gccaggcgta cgcgaagctc tgggagaaca cgatccactg gctcatccag gatccggacc
     1741 tgcgcaattt gcacgtcgac agcgatgccg tcgagtacgt gcccggccaa gcggtgaaag
     1801 tgaccgtgcg cctcctcggt cgcgattatc agccgctgcc aaacggcgtc gcgtcgctcg
     1861 tcgtgaagcg cggcgcggat ccgaacgctg ccgaacaggt gcagcaaaca aagctgacgg
     1921 tcggcgagga cggcaccgcc acgtacgagc tcggcggcct gacgccgggt gtctaccgcg
     1981 tgctcgggag cgcgaccgtc gcgggccgcc aggtcgaagc gagcgatatt ttcctcgtcc
     2041 gcgagggtgg cagcgagctg gaccgcccag tgggcgatcg cgacatgctg aggtcgattg
     2101 cagacgcgac cggcgggtcg gcgctcggcc ccatcgacga gctgccaagc catctgaagc
     2161 tggatccgcc gcgcgtcgta cgcgtcgatc gccgcacgga tatcgagctg tggagccgcc
     2221 caggcctcct gattctcatc gtgggtctgc tcgggctcga gtggctcttg cgccagcgta
     2281 gtggctacct atagataaag gcacccagat gctccagctc gacagcatcg tcaccgacct
     2341 gatgttccag tcgacggcct cgatcgacca gcaaacgctg atcgaaggcc tcggcggtga
     2401 gacggtactg cagacgaagc agctgctcga cgtcgaccgc ctcgacccac gtgccgagat
     2461 ggagctctcg ccatcgatgc cgttccgccg tcgtggcgag cggcgcctga tgatcatcga
     2521 tgaccaggcg acgacgcctc gcgaagatcc gcagaacgat ccggtcatca agacagacct
     2581 gcgcccgttc aaagaggcgc tgatcgcctc ggcgcagaag cttccgctcc gcctcggccg
     2641 catcttcgcg ctcggcgcgt ggatggacgc ggtgatcgtc gcgcgctcgg ttcgcgatct
     2701 gcgcggcatc acgctgctgt cgtgggcgct cgacccgcac gtcgacatcg acggcaagcg
     2761 ccgccagacg ccgctctcgc agaaagaatt cgaagcgaag ctcatcgcct acgagaagcg
     2821 cctcgacgag ctcgacgagc agcagatcct cgatcgcctc acgggcctcg cgacgttcga
     2881 gcggcgcggt gacctcgtgg tcgtcgatgt gctcgagccg gacggcacgt gggatcaacg
     2941 caagtcgatg ctcctcgagc agcagctcgc cgcatgggac acgttctcga tgatcccagg
     3001 cgcaccgtcg cagtctgcga agaagccagg cgcgccggcg ccgtcgaatg gcgagcccgc
     3061 aaaagccgcg gcgaagtccg agccggcgaa ggccgctgcg cccgcgaaga ccgctgcgcc
     3121 tgcgaagccg ccggagcccg tcaaagagga gccgaagggc ccgccgattc aagcgcagga
     3181 aatcgacggc aagatcgtgc tcgtgttccc gccggagcgc ttcgatctcg atgtcgccgc
     3241 agcgctgggc aagcgcgatt gggacaacgt cgtccgccgt tccgacaaca tgaccggcgc
     3301 gatgcgcgac aagatgcacc gcgatggcgc gtgttggatc gcgccgctcg agttcctctc
     3361 cgaagtcttc attgacggta agccgctcac gaagcagcaa ttcgagaaag atgcgcagcc
     3421 cgtcgacggc gcgcgcgcgc tcgaagtcca cttcccgcgg tttggcgaag tgatcctcct
     3481 cgatgtcccg ggcaagggcc gctacgtcac gtcgctcacg gatcgcgcag aacgcgcgct
     3541 atcgctggtg aagtaaatgc ttcgcgtcgt cgtcgtgctc gctgcgctga tgagcgtagc
     3601 ccgtgccgac gacgatctgg gtcagcgcac ggtcgtgttc gcgcgcggca cgacgctcta
     3661 caagtccgac gcgaagggca agaacgagac agagctcgcg acgctgccgg cgaagtcgac
     3721 cgtgcgtgca ctgcgcaccg acgcgctcgg caagatcttg ctcgccgata tcggcggcac
     3781 ctggtcgtgg atgccgctcg acggctcgac gacgtcgctc acggatctgc cgtgcgacgc
     3841 aggccccgcg cagctcgcgg aggacggcct gtgcgtgctc tgccgtcaac gaaagcaagg
     3901 atcgacaggc ggctcgatca tcgtcaatct agcgaccaac aaggtcacgc ccgtcgacgt
     3961 tccaactacg ggcgcgcgtc tcgcaggcat gggcgcggag cgcaagctcg tgtgggccga
     4021 cgacaaaggc gtgtggaccg cgtcgcccgc aacgccgaag aaagcgacaa aggccgcgcc
     4081 tgaagcgccg ctgcgcaatc tctcgccgag cccggacggc gcgcatgctc tcggcacgtt
     4141 caatggcgaa gtgttcgagt cgcccaaggt caaaaagccc ggcgagatcc tgatggagtt
     4201 cgcgctcgac ggtgatggtg cgcggcgcaa gctgatccag aacggcgtac cggtcgagtg
     4261 gtcgcacgac tcgaagtgga tcctgctgca agacggctcg agcgcgtgca tcgtgcaagc
     4321 ggcgggtggc cagtacaaat gctggaaggg ctacaccgcc gccgctctcg cgccggacgg
     4381 aaagtacgcg ctgctgctcg gcaaccgcga cggcagcaag aagcaggcga cgaaggacga
     4441 caagaagaag tcgtcaaagt cgtcgaagaa gaacagcaag aagaagaaag agcccgaccc
     4501 gccgccagag cccgagaccg acgagggcgc cgatgtcgaa ggcgaaggtg gcgacgatga
     4561 gatcccgacc gacgacgtcg ctgtcgcgcc gccgagcggg ccactcgcgc tctacatcgc
     4621 gcagctcgac ggtgcgttca cgaagtctcc gaagctcctg tcgaaggtcg tcgacggcgc
     4681 cgcggtcttc gttccggcgc cctagaaaac gagcagcaaa acggcgagca ccgcaacggt
     4741 gcacgcaagt agcgcgatga tcgcgatcat gtagatgcga gagttgtccg tcttcgggcg
     4801 gcgcagcgtc atttcgagct ggctctgcct cgtggtctcg tcgagctgaa atgcggagac
     4861 cggcgtatcg acgcgagcgc gcggcttact gactgcggcg acctctcgac tcgaaccgcg
     4921 cgcgagctga gtactgctgc tcgtcgcttg gctcgtcatg tttgccaatc gaaaactcga
     4981 ccgtcgacgc gcgcgctgct tcgcccccac aaggccatca tgaaatatga gtcagccttg
     5041 gtacagatcg agatgggtta acgatcgggg aaacgacgct gcctacatgt gcgctcggcg
     5101 gcacgccacg actactcgtc gctcgcgacg atcggcgcgc acgcgagcgc tttgcccggc
     5161 tttcccttct tgtcgtcgtg caagacgagc gtgtggccga tgagcgtctg ttcgccttcg
     5221 aggtgggcag cgagctccgt gttgtcgagc gcgccggtga gatcctcgag aacgacgagc
     5281 ctgaggtcga catccttcgt ctcggcccac gtgggtccgg gcttcgcgca ttcgctgccc
     5341 tcgtgaatca cgtagtgata tgtgcccgct ttgacgccga ccggtagatc cgcgagcacc
     5401 agcgcgttgt cgccttgccg ctgcatgaac gcgatgacga acggctcgag cttgctgccc
     5461 ttcgtcggag tcatctccgc ctttgcatgc aggatgaccg gctcctgctc cttcggagcc
     5521 ggcgtcaccg cggcctcgcg cggcaccggg gacggtttcg acgccgtcgg tgccatcggt
     5581 ttcgccggtg gaggcgatgc gccgccgcac gcgcaaatcg ccagaacgcc aaggattcgc
     5641 cgcatccctt gacgctacac agagccggca ttacttggcg agctaggtct atcgaaacca
     5701 acgcacacct atgctggtcg tcatccattc gggggagacg ggtgtcgaaa ggggagcgca
     5761 ctacggagca cgcaccgtcg agctggcggt gaacgggttt atgccgctgc aaggccgcga
     5821 cgagctgggc aagatcccgg tcgacgttgc ggcctacctc gtcccgcacg cagcgcgcag
     5881 ccggcgggag gcgcagatcg cgaatatcga gctctcctcg gcggtgctcc tgctcgtccc
     5941 cgaagccgaa aaggcctccg ccttcccggc cacgcgctgg ctcctgaccg agattcgcaa
     6001 tctcgggcgg cgccacttcg tcgccgaccc gaagtcgagc accgacgcgg tagccgagtg
     6061 gctgtgggcg atggcaaagg cgccagagcc tctccgcctc ttcatcaccg gtccgcgcgg
     6121 gacgcgctgg gatcgtggcg agacgtttgc gcgccggctc gtgcagacgc tcgcgatgga
     6181 gaagccctaa ggcatgtaga gaacgtcgtc ggcgaggatc cagacgttga ggtccggacg
     6241 cttgcccgac gcgatcaagt cgtagctgat gttgagcgac tgggactcgc ccttggagtc
     6301 cttacggaag aggaccatcg agtcgcgctt tgcaaagcgc gtgggcccac cggcgagcgc
     6361 gatcgcatcg gacaccgtca ggtagctcgg gctcgagtat tgcccgggct tgccgacctc
     6421 gccctgcacg gtgaaccgat agctcatgta gctcttcacc gtgatcgtga cctcgttggg
     6481 gctcgcgatc ttgatgaagt tctggagcgc cgtcgtgatc ttgtcgtgga ctaccttcgg
     6541 cgtctggccg gcggccttca cgtcaccgac gagcggcatc gtgatcgtgc cgtcgggacg
     6601 gacgaccgtc tcggtatcga gatccttctg attccagacg ctgatctcga ggacgtcgcc
     6661 ggcaccgagc actacctctt ggccgcgtgg atcaaccgtc cagaacttcg cgacgcttcc
     6721 gaccgggggg accggaggtc cgcacgccac caacaacgca atcaagacta aggcggttcg
     6781 catcgcctgc gacttttccg caatggccac cctcccgcaa gtgtgcgggg gcgcccatca
     6841 ccgctagatc tgcgttattc agcgcgcagc cagagcatgc gcgtgatttt cgagaacgcg
     6901 gacaggtcga ccttgttgcc tgcggtcgtc gtcgcactcg tcatcttctg ctgcttttga
     6961 ccgccggagg cgacgagcgt ggtgccggac tgtgcgagcg acgtggcacc ggcggcgatc
     7021 ggcgtggtcg tggagagcgc cggcgcaatc gccgtggtgc cgagcgcgta ggtcctgaga
     7081 gaacccgtat tcgctgtcgc accgtagctc gaaccgttgg cgtcttggct atccgtcgtg
     7141 acgaatagct gcgtgccaac gacgatggcc tgcgcaaagc caaagccagt gaagctcgtc
     7201 gcgaaactga cggcgcacgt cgcgcaagcg gtcgtggact cgctcgccga gctcgtcgcc
     7261 gcttggttga gcttcacgcc gacgacgtat tgggtccccg aggaccagag cccagtgccc
     7321 gtcgtatcgg tgtgatcgac gtaggcgccc gacgagacca cggcgaactg cgagctgccg
     7381 ttcgaataga tcgccggcgg ctggccgatc ggacgcttcg tcgtcgcgtt cgtgccgaac
     7441 gcgaacagcg gcgtcgtcgc acccgtgcgg ctcacacccg tctgcgaatt gaccatccag
     7501 agccggccgt agaggtcacc gaacacgaag tcgctgtatt cgcccgtgcc gacaatgtcg
     7561 acgccgaccg cgccgccggg gatacccgtc gcagatggcg acagcgtcgt cggatagagg
     7621 ctcgagaact tccagatttc ggcacccgtc tcgacggaca gcgccttcac gacaacaccg
     7681 gcaccgccgc tgccaccgtt gcgcgtctcg acgacggcca ggttctggag gcggccgccg
     7741 acgcgcaccg cgcccatcac gacgcgaagg cccgcgccga tctccgtcga tccaagcgag
     7801 ctcggaggtg tgacttccca caggatcgtc ggcgtcgaag ggtccgtcac gtcgagtgcg
     7861 tagacggcgg gcgtgccgtt aatggcatag ccggtctgga agatgaggac ggtcttccag
     7921 ctgcgcgatc cagtcgccgg gtcattggtg aagtcgccgt acatgtccat cacgcgaatc
     7981 gagccatcga tgcgttggtc gttcgtgcgg atgtacggaa gctgaacgcg cggcaagaat
     8041 gcccagagct cggtgcctgc cgtcacgccg ctcgccgagc acaggcccga cgtcgtcttc
     8101 tgtgcacacg ccgcgtggag catgccgtcg agaccgcccc agtagacgat cgtcgcgcgc
     8161 gacgcgctgc cggctccgtt cgagctcggt ccgacaacgg caacggacga gcgatcgacg
     8221 ccgccgatcg tgcgtgtcgt accacccgcc ttgctatcga ggacgaggtt gatgagcgac
     8281 ttgcgaagcg gcgtcgtcat cccagcgacg gccgtgctcg gcaccatcag ggcaccgatg
     8341 gagtcgatcc gtgtcgtatt cgtgtcgtta taggcgagct gcgtcttcgt cggatggaac
     8401 acccgaccag tcgcggagct cgtcaagttc gtgaacacgt agcggcacgt gccatcgggc
     8461 gtgctgcacg tggcattcgg tgcggggaca ttcgcgccgg cgtcgaagag cgtgttgcag
     8521 ccggaaggca cgcccgatgc ggtgcagacc gtgttgccgg tgatcgatga cgccgtcgcg
     8581 ccgatcgtcg agaccagacg ggcacggacg tgacccttga cgtacgggaa ggcgaacgca
     8641 gcgaccgtgc cggtgccgga cgcgcccgac gacgagatcg aggtcttcgt gcccgggctc
     8701 tcgaacgagc cttggacgag gacgtcgacc gcgctgccac catacgacgg ggtgagcgac
     8761 gcgatcaccg gcgcgccgcg tgccgtctcg tactgacacg ccagctggct gctcgtgagg
     8821 ctcgcgagcg ctgcgtcgta gtgaacgatc gcgttgttac cgttgaacgt gaccttcttg
     8881 ccgacgatcg cgccgtatac cgcgccgtta ccgttcacgg tgatgtcagc cttcggacaa
     8941 tagaatgcga acgcgccgtt accaccaccg gtgaccgtcg cgctcgtcac cgagtcggtg
     9001 cacatgaaga tgagatcggt cggcttgccg atgttgccgc tcgcatcggt gttcgagatg
     9061 ccgttgccgg cgatgctgac ggaggtaccc gtcacgtaga tatacgccgt tccggtgccg
     9121 gcctttttga ccgaggcgtt accgccgatc gaccagttgc ccgtgaagac gtagtcaccg
     9181 gcggagacgg tcagaacttc ggtcgcgtta ccgctgagcg aaacgccgcc atatgtcttg
     9241 ttcgccgtca gggtgacggg cgcggacgtg acactgcatg ccgtcggcga gcctgtgcat
     9301 gcgccgatcg agccgttcgg ccacgtcgga gcagtcaccg acgacagcgt gatcgccgac
     9361 gactgcgagc tcgtcgaacc gcatttcgac gagccgccgg agccaccgat cgccgtcgcc
     9421 gtgctgccgc cgacgccgac ttggcaatcg ccgttcacct tgccgttcgg accaaccgag
     9481 accgcgctgt tcgtgatcgc gttggtgccg acggcgccga ggcacgagtt cggaaacgtc
     9541 gcggtgctgc acttcgtcga cgcgtacgaa ccgctcgccg agttgtagct atcgctctgg
     9601 ccgttgccga tgtcgacgcc gatatcgccg aacgcggcct tgttgaaacc ggggatcgag
     9661 catccggctt cgacgagacg cggcgtcacg ataacgagcg cagcagcggc tatcgacaga
     9721 caaacaacgg gcagcctcat ggtcaccatt ttatatgctc ctggtcgcgc agcaccgtcg
     9781 aagatcgagg agatacgcgt gcgtacggct cccacatcgc gctactgcgc gaagcttccg
     9841 ttgccgtcgc aaccgccgtc ttccttgtag tcgcagctat tacccccggt gtactggacg
     9901 agctccatca cctgcttggg gttgtccggg aacttcgtgc acttcgagat gacaaacacg
     9961 cgcagatcct ggtctttggt gaggtcgttc gccgtcggag cgccctcgtc gtcgttgtcc
    10021 ttcaagtaga cggtgaagtc gtcgctgccg tcgttgtcga tgtcgcggtc gatcgtcgac
    10081 agaaagctag ggaggttggt gttgccgccg gcggaggcgg taatcgcagt accccactgc
    10141 gcgaaattag ctttgacgag cgcgcgtgac gacgcgaggc ctgcttccgc gcagtgcagc
    10201 gatgtcatgc cggtgcgcga gaggtccgtc gagcgattgc cagcgagctg catcgacacg
    10261 agcaccgcgg cgccggcaag catcgacgcg atgatgatca acgtgacgat catcgcgctt
    10321 ccacgctcgg gatttcgctt cgtcatgggg aacctccaat gttgcgcatg tcgacgatcg
    10381 ttcggagcac gcgacgacga taagcatcgg gcgcggtcga gccgatgcgg ttttccgcac
    10441 ccgggcgata gtagagaggc gcgttaccga cgagcggctt cgtcgcgcgt gccacgagcg
    10501 tgatgcgcac cgcgcggatg ctgccggtcg agggcatcgc ctcgccggcg aagttgaacc
    10561 accattcgtc atcgccggcg cctaagccgt tctcgctgat cgagccatcg ctgtcgccat
    10621 cgataccgac cgcgatctgc atgtcttcga tgccctcggc gagcggttcg ggctgaaacg
    10681 cggtgcccgc ggcgtctgga tccatccaca gcgtcgggat gccgtcgaga tcggcgatgt
    10741 agaagtgagc gtactgcgcg cgaatcacga gcgagccgac tggatatccc gccaccgaac
    10801 cagccgacgg gaccgtaaga ccagagcacg gcgacgcaaa cgtcagcgtg ttcgcactcg
    10861 tgtcgacggt gtcgacatgc aacagcgtgc cgttcgtgaa gttcgagatg atgacggcat
    10921 cgtcgtcggc gaactggctc gcgttcgtca cgtcgatgtt ctggctcgtg ttgtcgtaga
    10981 tctcgcgcgt cgacgtcacg accgcacccg cggcgtacat cagatagagc tcgtcggagc
    11041 cactgctgct gtcgatcacg ttgatcgcgc cggtgttgca ggttccggag tcttgtagat
    11101 tcgcgggcgt cgacacgccg gggctcgctt ggcgaagtgc gtcggcgagg aagtcgagcg
    11161 gaacgcgaac cgcggactcg gcggcgagcg cgcggcgctg ttctgcgtag ccctgcgtga
    11221 ccgcgacgcc gatcgagagc agcccggcga ccgcgaacga gaacagcacg agcgagatca
    11281 tgagctcgat gagcgtgaag ccgcgctggg tggtctcttg cttcatagcc gcatcccgta
    11341 gacggagacc gcgtagctca tgcaggtggt gaccttgcag aacttcgacg agcaatcggc
    11401 atcggtggca cacgtctgcg tcggcacgtc gtcgtcgtcc caccccactt tcacctcgag
    11461 cgtggtaacg ccgccgctcg acgacaccgt ccacttgcgc tcgaagatgc tgctggtgtt
    11521 gccggtaacg ccctggacgt cgatcatggt ctcgctgctg ccggtgggcg ttgccatcgt
    11581 ccgcagcttc tcgagcttgt cgtttgcgag cgccgaggcc tcggtctcgt gacgcgagcg
    11641 accggccgcg cgtccttgcg cgacgaacaa gccgatgatg ccgatgactg cgatcgccga
    11701 tagcaggatc gcgatcatca cttcgatcat ggtgaagcca tgctcgctac cacgtctcgc
    11761 gtgcataggc gctccccgtc gctttgtaga tgagcactcg atactggcga gcaccttgcg
    11821 tctcgccgat gtagatcgtg ccgccgctgc tcgacccatc gggcttgaac gtgatgctca
    11881 cgtcgaggtt cgcggtcaac gcgaccgaat ttcccgtcga cgtttcgggc tgaccatcga
    11941 cctgatagat cgtcacgccg ttcgggatcg acacacgacc gacaaactcc ggcgtcgcgc
    12001 tcgtcgtcgc aaagcccgtg atggggctct gccagatgaa catctcttgg ttcttgatct
    12061 ggagcgtctg gatccggcgc gtcgacaccg cgcgcatgcg cacccagcca accgtcgaga
    12121 cgaccttgtc ggagacgttg tacgcgttcg cgccgtacgt gcggccgctg aggctgatga
    12181 ggagcgtcgc aaggatcgcg atgatcgcga tgacgaccat cagctcgatc agcgtgaatc
    12241 cgcgttgaag acgtgcgcgc atcatttggc tgcgttcgtg acctggatgt tcgtgtcgac
    12301 gctgctctcg taatacatcg cgttcgtgcc gccttgaccg tcttcgtcgc actcggcggt
    12361 cgcgtaccac cagcccgatg cgagcgtggg ctctgcggcg gcaacgcctt gcgagttctt
    12421 gaatcccgtc ggcggcgtca gcgtggtgcc cttcgctccg acgaccaccg tataggcgca
    12481 gtgcatcttc gattccggcg cagcgacgcg agcggttacc cacgcagcgt cacaatctcc
    12541 gtcgacgatc atcttgccga tgttcgcgcc tgcgacgctc ggtccggtcg tcggacacat
    12601 cggcagagct aggtacgagt tgaactcgtt cttgtatgtc tcttgcttcg tcgagatcgc
    12661 agtgaacatc gcgttcactt cggcctgccc cttcgccttg cgagcttcgc caacgaacgt
    12721 cggcaccacg atgattgcga gcaccgcgat gatcgcgacg accatcatga gctcgatcag
    12781 cgtgaaaccc ctctcagctg tcgtttcgcg cacctggcca tcgtagcacg cagctacgga
    12841 ggacccgaca attcgcctgg ggtctcgatc ggttacttcg acttcgcgtt cgccggtgtg
    12901 atcgggttgc catccacgtc gagctcgacc agcccacccg ctcccacatc acccttttca
    12961 gcgagcttct ggagcgcctc gcgcagcgtc agctggcgtc catcgacctc gtacggcttc
    13021 ttgcgatcct ttggcagcgc gcctgctttg ggatcgtcga cgatcatctt tgcatcgcca
    13081 tcgccgacga cgaggaagac ggcgtccttc tccttgaggt gcttcgtcgc ggactgcttc
    13141 gcctgcgccg ccgtgacctt cttcaccttc tcgacgtacg tattgaagta gtcgagtggg
    13201 aggccgtagt acacgaggct ccgatattga ccgagggcgg cctgggcggt cgcgaaccgg
    13261 ccggggagcg cgaggatcgc gttcgtcttc tcgcgatcga gctcttcatc ggtgaccggt
    13321 cgcttgccgc tcgccagctc gaccagctcg cgatggacct cgcgcagcgc ctgatacgac
    13381 gagtcggcct gcaccggcgc gttcaccgtg agctgaccga actgcttcgg cgagtagcca
    13441 aagccaccgc gcgcgccgta cgagtagccc ttgtcctcgc gcaggttcat gttgaggcgg
    13501 ctcgtgaagc tgccaccgaa caccgcgccc atcatcgagt tcgcgaagta gtcgggcgcc
    13561 gttctcgtcg ggccgaacga cagcatcatc acgttcgact gcgctgcgtt cggcacgtgc
    13621 acgaagaaga tcttgccctt catcgtcttg ggcttcggca gcgcggcgag cttcgggccc
    13681 gcgcctgtcc acgcgccgag cggcgacttc tcgaactgcg cgcgcagctg ctcctcggtg
    13741 agatcaccga cgacgaatag ccgcgcgttc ttcggcttga gccacttgct gacgtacgcc
    13801 ttgcaatcgt cgagcgtgac ggctttgacc tcgtcctcgg tcagcacgct gccgagcgga
    13861 tgctcgggtc cgaacaagat cgcgccggtc acgcgaccgg ggatcgacgt cgggctcgtg
    13921 cgcgattggc ggactgcttc gatgcggcgc ttcttcaagc gatcgaagtc cgattcgcgc
    13981 atgccgggcg tgaggatcgt gtcggcgaac aacgcgaacg tggtgtcgag atgcttcgtg
    14041 agcgatgcga gctggacgcc ctgcgtgtcg tcacctgcgt aggcgttgat gttcgacgcg
    14101 acgtcggcga gcgcttcgct gtactggatc ttgtcgagct tctgcgtgcc ctcggtgagc
    14161 agcgacatgc atgcgcccgc gagaccgtcc ttgcccttcg ggtcggtgat cgagccgccg
    14221 tcgaagttga ggtccatcga aacgatcggc aggttgtgct gctcgacgag gtagacctgg
    14281 atgccgttct tgagcttgaa cggcttcacg gccggcagct tgaaatcgtg cggcgcggtg
    14341 ccttgcggct gcgtgcgacg gaaatcctcg tcgggaaact gaagagtctg cggctgcgca
    14401 gtctgcgtga tcgccggcat gggagtctcc gcgggcttgg tttgaggagc gggcgcgggc
    14461 gtcggctccg gtgccggcgc gggcgccggc gtaatcggag gattcggaac ggtgcgacac
    14521 gctgcgagca gcgcgattgc cattaacttc ttcatttgga acctccggcc gggttcgtcg
    14581 tgatcgtgac catgctcgtc gggacgaggt acttcgctgc cgcggcacgg atgccgtcgg
    14641 gcgtcgcctt gcgatagcga tcgaggtccc agctgagctt tcccggatcg ccgaggaatt
    14701 ggttgtacgt ctggaggacg ttcgcgcgcg cgttgaggcc ctcgagccgg tagatcatgc
    14761 tcgcctccga tgcggtgacg acgcgctcga tctccttcgc ggacaggttc tccttcgtga
    14821 ccttcgcgac ttcgtccgcg acgatcttct tcacctgctc catgtcggct tcggtgcgga
    14881 gcgtgaccgt gacatcgaac gtgccggaga acgtcgagcc gtcttggctg acactgacgc
    14941 gcgtcgcgag ctggttgtcg aggacgagcg ccttgtagag ccggccgcgg ccttccgcac
    15001 cgagtgcgtt ggctacgatg tcgagctccg cgtcaccggc cacgtaggcc gcgggcgagt
    15061 gccacgcgaa cacgacttgg cgctgcttgg cgaacgtatc gtcgaccgag acgctcgtcg
    15121 ctttctgcgc gggcgccggc accttgaccc acttcggctt ctcactcgcc ggatacgtgc
    15181 cgaaccattt ctcgacgagc ttcttcgctt ccgccttgtc gaagtcgccg acgatcgtca
    15241 gcgtcgcgtt cgccggcacg taccaggtct tgaagaatcc cttcacgtcc tcgagcgacg
    15301 cgctcgtgag gtcctcgtgc ttgccaatcg tcagatagcg atacggatgg ccctcggggt
    15361 acatcgccgc gtacagcgcg aagagcgcct tgcgatacgg aacgttgtcg tagttctgac
    15421 ggcgctcgtt gcggacgacg tcgatctggt tgtcgagcgc tttgcgatcg acgagctcga
    15481 gcaagtgacc catgcgatcg ctctcgagcc agatggccgt ctcgaggaag tgcgacggaa
    15541 ccacttcgta gtagttcgtg cgatcgggat tcgtcgtccc gttcacttct gccgagccga
    15601 tcttgcggag gatctcgaag tgcttgtcgc tgccgacgtt cttcgagcct tggaacatca
    15661 tgtgctcgaa caggtgagcg aagccgctct tgccaggcac ctcgttgccg ctgccgacgt
    15721 ggtaccagac gttgaccgcg acgagcggca cgcggttgtc gggcgacagg atgacttcca
    15781 tcccgttcgg cagcttgtac ttctcgaact cgagcttcgg atcgtccgcg tgtgcggtgg
    15841 acacgctcgc ggcgagcacc gcggcgatta gcaaaggcct catggccgag aagctagatc
    15901 aaagctcgag cggctgcacg ccgaggacga cgtgatcgcg actcacagcg tcgagccaaa
    15961 gcagcaaacg ccgcgtctca tcggcggaga cgtcgatttc gatgatgcgg cgaatcgggc
    16021 tgcccgcgag cgtgcgcgcg atgtcctgct cggcggtcga ccacgcctgc gcgaggggag
    16081 tcgacttcac ggggaggcct tcgacctcga cggagatgcc catcacgaac ttcgcgaacg
    16141 ccgggttcgc cgcgacgatc agcccctgcg gcgagagcag cgcgagcggc aaccggcacg
    16201 catcgaaggc cacgcgtgcg aattcatcgc gcttgttcgt ctcgggcggc ttctgctcga
    16261 tcacgacacg gcgcgcaggc gcagcgccca cacctcgtcg acggccgatg ccgagcatgt
    16321 ccatcacggt cttcagctcg tagatgaacg ccgccatgtc cttgtgccgc tcggccgggc
    16381 gcttcgcgag cgcatgcagc acgaggttct cgacggcggg atcgaggcca ccttcgatca
    16441 gcgacgacgg cgggcgcggt ttctcgtcga gatggccctg gaggatcttc tgcacggcgc
    16501 cgtcccacgg cacctggccg gtgagcagct cgtagagcag aatgccgacg ccgtagacgt
    16561 cgctcgacgg agatgcgggc tcaccgcgga tgcgctccgg tgcgacgtaa tgaggcgtgc
    16621 ccgagagcga cgtcgatgcg cggggtccgg tgagcggacg agcgaggccg aaatcgagga
    16681 gcttggcgac catcttcgat cgcttgccct cgatctcttc ttcttggatc aggatgttct
    16741 cggtcttgat gtcgcagtgg acgacgttct gcttgtggat gtagtggagc gcctcggcga
    16801 cctgcagcac gatctcgctg gccttcttca cgctgagccg cttttcgcga aacaggatgc
    16861 ggtgcagggg ttcgccaccg acgaactcca tgaccatgaa catgccgacc ttctcgtctt
    16921 caccgaagtc gacgacggac gtgatgttcg gatgtgacat cgccgaggca aagcgcgcct
    16981 cgcgatagaa cagctcgcgg gcctcgtcgg tctcggcgac ttggttcgag atgatcttca
    17041 gcgcgaaccg gcgcgagaga tggatgtggt tgacctcgta gaccttgccc atcccgccct
    17101 ggccaatgcg cttcacgatc tggtagcggc caccgatcag cgagctgtgc tgcgcggtcg
    17161 tcacgcgaac catcgcgctg cgcggaggcg caaccggcgt gtgcataccg gcagccggcg
    17221 ggcgcgtgcc ttccctgccc cggccaaacg tggcgtctcc gccgcggacc ttcgacgtat
    17281 tgggcgtgcg ctcctgcgtg gccggccggc cgacgcgggt ggtgtcgtga cgatggcgcg
    17341 gaatctcgcg ggcaccggag agcgagtccc gacccggagc gagcgcgcga tgatcgatgc
    17401 ggacttcgct cgaatcgtcg acgttcacct cgggaagccg aggcaggtcg tcgtcgtgcc
    17461 cgcgacgaat ccgcgagctg tcctcttccg ggggtggcgc gggcagcggc aagaccggcg
    17521 ggcgacggcc cgagggattg cggtgatccg ccacgtcccg atcctacacg atgtacattg
    17581 gttcgggcat gcaggagctg tggtcgagcg agagctacga gccgcacctt ctgtccctac
    17641 cgttcgcatt tgcgcccgtc gcgatgctgg tggtcattgc gtacgccctg gtcatgcggg
    17701 gagagccgaa gctgcgcgcg tggatgctgc tgcacttctt cgcgctcttg ccctacgcgc
    17761 tgacgatgac gctgtcgccg tcgctcgtgt cgcaggaggt cgccgatcac ctcttccgca
    17821 tcgcaggcgc ctgcattccg atggccgcgg cggccggctg tggcttccag tatcagctga
    17881 tcgggcaggc ccaccgcagg aggtggtggg tgtggggcgg cgttgtcgtc gccctcgcat
    17941 ggctcgtgat cggcacgacg acggacctga tcgcgaacgg cgtccgctgg ctgcctgcga
    18001 agttctggta cgccaatgca ggctcgctca cgtggctgat cctgctgacg accgtgatca
    18061 acgcgacacc ggcgttcg
//
LOCUS       DATLBZ010000622         3606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8744838, whole genome shotgun sequence.
ACCESSION   DATLBZ010000622 DATLBZ010000000
VERSION     DATLBZ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8744838"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1229)
                     /locus_tag="VMZ53_30610"
     CDS             complement(54..1229)
                     /locus_tag="VMZ53_30610"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HUS32908.1"
                     /translation="MATDDDDFAAMFAESERDKPKTKRPKVGDMVKGKVVNVGKDAVF
                     VDLGGKAEGQLEKDQVSDREGKLLVKIGDVIEARVVSDAGGILVLRTKLSRGPQASAE
                     LVQAQELGIPVEGLVTEVVKGGLSVDVAGVRGFCPASQIDNRFVDDLAKFVGQKLTFR
                     VTRYEARNLVLSRRALIEEEQEKKAGETRKRLVPGAVLRGKVVGFKPFGAFVDLGGIE
                     GMLHVSELGFTRVEKPEDVLTLGQELDVAVLKIEPAAEPERDGKKQHDRISLSLKALG
                     NDPWRDSTASLAEGARVKGTITRLQPFGAFVEIAPGVEGLVHISELGAGRRINHPKEI
                     VSIGQQVEATVLAVDSEKRRLSLSLASSKDGSAEDVAAMPRAPQKLGTFGDLLQRKK"
     gene            1436..2197
                     /locus_tag="VMZ53_30615"
     CDS             1436..2197
                     /locus_tag="VMZ53_30615"
                     /inference="COORDINATES: protein motif:HMM:NF027619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS32909.1"
                     /translation="MNIHSLAMTTAKTSTSGAPKGKPGATSQPTHADSASHPGTWSTE
                     AKQPPARKSFASLERKGAEKPAPPADKREAIMTAALDLFVERGFHGTAVPEIAEKAGV
                     GAGTIYRYFESKEALVNALYRQEKQRFADRTITDFPKTTIARELFRTMWMRMADFAAA
                     NPKPFVFLELHHHAPYLDEQSTALEQRMLELFTGVVVAAQARGELKKGPPRLLMGLVM
                     GAFVGVIRSCVEIAQPLEEADWTLAEQCIWEAIRA"
     gene            2284..3300
                     /locus_tag="VMZ53_30620"
     CDS             2284..3300
                     /locus_tag="VMZ53_30620"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS32910.1"
                     /translation="MSNTSKIVLITGATAGIGRMTALHLAKRGHHVIATGRKPAELQK
                     LALEANQELAALGAKALAKGQNGRKHVTGKLDTLLLDVTSLASITAAAEQVNTLTAGQ
                     GIDVLVNNAGFGALGPTSEISDAEMRRNYETNVFGLMSVTRAFLPRMMQRRNGRIVNV
                     SSVGGRITLPYFGVYNSTKYALESLSDALRYELRPFGVDVTMIEPGVIRTNFEATAVA
                     NLDEFAKTPFAKSVAKYEKMSKTADRFASDPIVIAKAIARAVEARRPAARYVAPFSTN
                     FVILFARLFPTRVWDWAMRQVAYLNAKNLDVASTSSAKPAESPASAPFSSAKPAVDAR
                     ASAN"
BASE COUNT          664 a         1214 c         1116 g          612 t
ORIGIN      
        1 cagctacgcg agcgcagcgc gatacttctt gcagctacac gagcgcggcg caattacttc
       61 ttgcgctgca gcaggtcgcc gaacgtgccg agcttttgcg gcgcgcgcgg catggccgcg
      121 acatcctcgg cgctgccgtc cttcgagctt gcgagtgaca acgaaagccg gcgtttctcg
      181 gagtcgaccg cgagcacggt cgcttcgacc tgttggccga tcgagacaat ctctttcggg
      241 tgattgatgc gacgtcccgc gccgagctcg ctgatgtgca cgagcccttc gacgcccggc
      301 gcgatctcga cgaacgcgcc gaacggctgc agtcgcgtga tcgtgccttt gacgcgcgcg
      361 ccttccgcca agctcgcagt cgaatcgcgc cacggatcgt tgccgagcgc cttgaggctc
      421 agcgaaatcc ggtcgtgctg cttcttgccg tcgcgctcgg gctccgcggc gggctcgatc
      481 ttgagcacgg cgacgtcgag ctcttggccg agtgtgagca catcttcggg cttctcgacg
      541 cgcgtgaagc cgagctccga cacgtgcagc atgccttcga tgccgccgag atcgacgaac
      601 gcaccgaacg gcttgaaccc gacgaccttg ccgcgcagca ccgcgccggg cacgagcctc
      661 ttgcgcgtct cgccggcttt cttctcctgc tcttcttcga tcagcgcgcg tcgcgagaga
      721 acgaggttgc gcgcctcgta tcgcgtcacg cgaaacgtga gcttctggcc gacgaacttc
      781 gcgaggtcat cgacgaatcg attatcgatc tgcgatgcgg gacaaaatcc gcgaacgccc
      841 gcgacgtcga ccgacagccc gcctttgacg acttcggtga cgaggccctc gaccggaatg
      901 ccgagctcct gcgcctgcac gagctcggcg ctcgcttgcg gaccgcgcga cagcttcgtg
      961 cggagcacga gaatgccgcc cgcatcgctg acaacgcgcg cctcgatcac gtcgccgatc
     1021 ttcacgagca gcttgccctc gcgatcgctg acctgatcct tctcgagctg tccctcggcc
     1081 ttgccgccga gatcgacgaa caccgcatcc ttgccgacgt tgacgacctt gcccttcacc
     1141 atgtcgccga ccttcgggcg cttggtcttc ggcttgtcgc gctcgctctc ggcgaacatc
     1201 gctgcaaaat cgtcgtcgtc ggtggccatg cgggcgcact ctacccaacg aggtgcgaga
     1261 gttaagggac ggacgccact tccgcaaccg agcgagaaga gcgggaaggg acggacgcaa
     1321 cttccgcaac cgagcgataa gggacggacg caacttccgc aaccacgcga aaaccgcttt
     1381 cgcgtccagc ggttatacga ttctgcgaaa aaagggcttg cagattcttc gcggaatgaa
     1441 tattcattct ctcgcgatga cgacggccaa gacgagcacc agcggcgccc ccaagggcaa
     1501 gccgggcgcc acgtcgcagc caactcacgc cgactctgcc tcccatccag gcacatggtc
     1561 gacagaggcc aagcagcctc ctgcccggaa gtcgtttgcg agcctcgagc gcaagggcgc
     1621 cgagaagcca gcgccgccag ccgacaagcg cgaagcgatc atgacggcag ccctcgacct
     1681 ctttgtcgag cgcggctttc atggcaccgc ggtgccggag attgcggaga aggcgggcgt
     1741 cggcgcgggc acgatttatc gctacttcga gtcgaaggaa gcgctcgtca acgcgctcta
     1801 tcggcaggag aagcagcggt tcgccgaccg cacgatcacg gattttccga agacgaccat
     1861 cgcgcgcgag ctgtttcgca cgatgtggat gcgcatggcc gacttcgccg ccgccaatcc
     1921 caagccgttc gtcttcctcg agctgcacca ccacgcgccg tacctcgacg agcagagcac
     1981 cgcactcgag caacgcatgc tcgagctctt cacgggtgtc gtcgtcgcgg cgcaagcgcg
     2041 tggcgagctc aagaagggcc cgccgcgcct gctcatggga ctcgtcatgg gcgcgtttgt
     2101 cggggtgatt cgaagctgcg tggagatcgc gcagccgctc gaagaagccg actggaccct
     2161 cgctgaacaa tgtatttggg aggcgattcg cgcgtagggc gcggatcctc cttaactgtc
     2221 tctttttttt ggcctttgca cggaatgaac attcattcca acgaacctcc aggagccctg
     2281 accatgtcga acacgtcgaa gattgtcctc atcactggcg ccaccgcggg catcggccgc
     2341 atgaccgcac tccacctcgc caagcgcggt caccacgtga tcgcgacggg gcgtaagccg
     2401 gccgagctgc agaagctcgc gctcgaagcg aatcaagagc tcgccgcgct cggcgccaag
     2461 gcgctcgcca aaggccagaa cggccgcaag cacgtgaccg gcaagctcga cacgctcctg
     2521 ctcgacgtca cgtcgctcgc gtcgatcacc gcggcggcag agcaagtgaa cacgctcacg
     2581 gccggccaag gcatcgacgt gctcgtcaac aacgcgggct tcggcgcgct cggcccgacg
     2641 tccgaaatca gcgacgcgga gatgcgccgc aactacgaga cgaacgtctt cggtctcatg
     2701 agcgtcacgc gcgcctttct cccgcgcatg atgcagcgcc gcaacggtcg catcgtcaac
     2761 gtgtcgagcg tcggtggtcg catcacgctg ccgtacttcg gcgtctacaa ctcgacgaag
     2821 tacgcgctcg agtcgctgtc cgatgcgctt cgctacgagc tgcgtccgtt cggcgtggac
     2881 gtcacgatga tcgagccggg tgtcatccgc acgaacttcg aagcgaccgc agttgcgaac
     2941 ctcgacgagt tcgccaagac gccgttcgcg aagtcggtcg cgaagtacga gaagatgtcg
     3001 aagacggccg atcgctttgc gtccgacccc atcgtgatcg cgaaggcgat tgctcgcgcg
     3061 gtcgaagcac gacgccccgc tgctcgctac gtcgcgccgt tctcgacgaa cttcgtgatc
     3121 ctcttcgcgc ggttgttccc gacgcgcgtg tgggattggg cgatgcgcca agtcgcgtac
     3181 ctcaacgcca agaacctcga cgtcgccagc acgtcgtcgg ccaagccggc cgaatcgccg
     3241 gcttccgcac cgttctcgtc ggcaaagccc gccgtcgacg cccgcgcctc ggcgaactaa
     3301 tcgcgcgagc gaagcccgga agtcgcaatc gctagcgagc taggcggcga gctcgtgacg
     3361 gatgaattcg gcgaaggcga gctaggcggc gagctcgtga cggacgaatt cgcgcaagcg
     3421 ggtacccgcg gtcactcgag ccgagcgatc cagtcccagg cgtagagtta ggcggcgagc
     3481 tcgtgacgaa cgaattcgcg aaagcgggcg tcgaagtcac cgacgagcgt acgtgtgaac
     3541 gccagccgtt gcgcaatggc gcatgaccag cgcgcgacct gaggcaggcc ctcgtagaag
     3601 tcgatg
//
LOCUS       DATLBZ010000623        11737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8746246, whole genome shotgun sequence.
ACCESSION   DATLBZ010000623 DATLBZ010000000
VERSION     DATLBZ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8746246"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VMZ53_30625"
     CDS             <1..585
                     /locus_tag="VMZ53_30625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32911.1"
                     /translation="AAVDASPEPAFMRGLAEAAAYTGDGPAALVFATTAAASSGDPAI
                     VWAAVAADLVAANQLPDSLTASRNALDLAGPDVLPAALDTAIASSRALGRTQQADALL
                     LQRAQLAPRKRSDDTEVLAALAAHRERPTASTAANLWVASRALPRDVESRAVLLDALD
                     ADDPRRETIRDELLLLAGDPDPARALAAAHALRD"
     gene            658..1311
                     /locus_tag="VMZ53_30630"
     CDS             658..1311
                     /locus_tag="VMZ53_30630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32912.1"
                     /translation="MSRAIRSPLLAPLQHAVPLHLAAPLLALFAAAACTGSIDDTARG
                     DDNSDGPDAGIDLGPGDHEAVARAKLWVDAKVPYCQAPNHQRDYDEACMMTCTRPDVA
                     AWDPYRSDCSGLVSWSWGLPPPGRTTSTLAPYNTDVSHSIDAADLQPGDAINNDHHTM
                     IFVGWLEPMTKARFYEEPGCSSSQPYARQYDASVMMMAGKTIVLTYRGTYTAIRQTK"
     gene            complement(1318..1575)
                     /locus_tag="VMZ53_30635"
     CDS             complement(1318..1575)
                     /locus_tag="VMZ53_30635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32913.1"
                     /translation="MDILVDAHKHSTQHRAEIEGSGRCGCFFCFRTFPPTLIKKWTDA
                     DQTALCPSCGVDSVLGSGSSHRLDDAFLRKMHMHWFARRSK"
     gene            complement(1754..2197)
                     /locus_tag="VMZ53_30640"
     CDS             complement(1754..2197)
                     /locus_tag="VMZ53_30640"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUS32914.1"
                     /translation="MSGDGDYKNPTPTCDILIELDGRPGTLVFIERKNEPKGFALPGG
                     FVDEGEWIADAAVREAKEETELDVEIIELFHVYSDPKRDSRKHTVSTVFIARAKGTPK
                     GGDDASRCVVCKPDELPAPLVFDHALIVSDYLAYKQTGKRPPPRR"
     gene            complement(2194..4464)
                     /locus_tag="VMZ53_30645"
     CDS             complement(2194..4464)
                     /locus_tag="VMZ53_30645"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUS32915.1"
                     /translation="MSEGAPPPPYRSGLATGAGVVLVAGLVLAIADVVHTGAAPLQVL
                     GLWSLLTLPVAIGAGLVIAAGNATWGSGWVRRLFRRLSEDDELDRTVSAILIAAVVVA
                     GVLILGVAKLSVGLVGDVQRKSVGGLLLGVIVVALVPVLALAVLPLFRVTRKITGVVP
                     SIGPLSRVLLLTFLGAVLVACAGLFVIFKKLDYQALNLGSLFVPAMMPILAIVIAIVF
                     YGPLSGVRERIPSRVILVSVGLMFAILIAVFGLRGEPSQKNQVAVLDRSYVGPRLIPV
                     LRKLSDHDHDGYSAFFGGPDCNDRDKAIHPNVIDVPDNTIDENCDGFDNQKAPTLPDL
                     PTGKNAPAPTLKGGDNVLVIFVDTLRYDRLGFTGYQRDGKSLTPRIDAFAKQSVVFKN
                     AYSQASNTPRSVPSFLASQYPTKLKVDAMEKNYPLVLDDNELLFEVLKPAGFTTIGES
                     SHFYFCDRQKYAETCSNVKNTDGNPMRTNAIQGADLWDNTGALPISGSNHDIAGPRVV
                     EKTKKKLAELAAGKQKFAMIVHLFEPHSTYMEHDGMAITERGTASLMQKYDYEIAFED
                     KLIGELLDELDTLKLAGSTTVVLMSDHGEAFGIHTFAGEKQFFHGMTLYNEVLHVPLM
                     FRVPNNKPREVSDVVELIDLSPTIASLFGVTPPKSWVGRSLVGALEGAPLPAKPAFSE
                     MPPSKSWPHDARSMISADGKHHVFHRISDSRWEVYDLAADPEERKNVVDSDTQAKQLQ
                     QQLASWEQSLLSGANQ"
     gene            4621..4932
                     /locus_tag="VMZ53_30650"
     CDS             4621..4932
                     /locus_tag="VMZ53_30650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32916.1"
                     /translation="MDCNEELSSVRYDVSSATTFNRLEIRLDGRLAAEPTFFEGVTAY
                     HYEHDDVFATYYDEIAITAFVDDAPIAAGSAEPAYRHDGMVHIAEGVLALQPLPAFAP
                     R"
     gene            complement(4929..6593)
                     /locus_tag="VMZ53_30655"
     CDS             complement(4929..6593)
                     /locus_tag="VMZ53_30655"
                     /inference="COORDINATES: protein motif:HMM:NF039677.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HUS32917.1"
                     /translation="MSERDVPPPSGPLARLRAHLAGPRGYRRIDALLSADDAPAAIAA
                     LSASEIYELVHEVGFEDGQALIEYATPSQIQGCFDLDGWNKDQLEVVSLKPWLAALIE
                     VGFEKVGEVWGALDAELRTLILQKQVLVYDTTMNEGPEEDNDEPIMTTPDRFFLLELK
                     GDDDTQRLTQQLVEDLYRADPQLARHTIMAARSEPPAELEEMAYRWRSGRLADFGYVD
                     FYEALDLFRPLPADQVHIGEGSQDRPIEDASSHLPVVIVEEVIGRSFLARAMAAIDDD
                     REAERLEGSLMVLVNKVLAAGRAKPGQSEVMRRGALYATATLSLGLDVVARGDLERAK
                     QALQSISLGRLFRVGYTVTHKLAKLATALAPRSPTAGSPAKDLVAGLCSPRPLFSRAA
                     DEPAMPGLRPFESQADLRRAGEILTGLTIRIALVEGLGVDVLAMGQAPEPRPELDDHI
                     RTALARSLVGGEELRGDALSQAELTQLRKTAFDASGKLTDIARRAGHAAINARLGAAQ
                     LAASGPLLGRLIDSWLDDLERILGGIKEGDVDPRFVEGVIVEVQRS"
     gene            6610..7854
                     /gene="tilS"
                     /locus_tag="VMZ53_30660"
     CDS             6610..7854
                     /gene="tilS"
                     /locus_tag="VMZ53_30660"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HUS32918.1"
                     /translation="MGTTPRDPVSTLRAAVKLWIDQLGAGLYGLACSGGADSMALADA
                     ACAVAGRENVVLVHIDHGLRDDSATVASELAAWAKGQGITAVVRRVDVRGESVEAAAR
                     EARYRAFGQLHEELGLSRILLAHTARDQAETVLMRMLRGTGPAGLAAMAAQRDVYVRP
                     LLEISREIVDDYIAARGLPTWRDPMNEDPAFTRVRLRTVVLPALRAENPAIDDALVRL
                     ARAAGEWRDAIDALAEPFATFPIDCEELATQLPAIRKRAVALALERARLGYEAAHLDA
                     IDALVMAPTAGEHSIDVPGARLVRSYETLEIRSDQISEIQSDLISGDYEVRVWRPGDR
                     MKPARLKGRSRKLSDLYIDAKVPRAIRAASRVVIRRADAVIIYAEHLGISVDARPNDS
                     EFLGSISFASQLKGPMLSNGRG"
     gene            7866..9818
                     /gene="ftsH"
                     /locus_tag="VMZ53_30665"
     CDS             7866..9818
                     /gene="ftsH"
                     /locus_tag="VMZ53_30665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HUS32919.1"
                     /translation="MRQSHKTILIWAILILMFVSIYSMFTDSSSKEKEVDVTTFRADL
                     LNKEKAKEIEEIAIEPGGHDNARYVIKKSNSSVREVVHAEFPGTITKEIYDAGIKYRV
                     KAKDDSSIWPQVLVWWLPMLLLVGIFFLFMRQLQSGGGKAMSFGKSKAKLLSDHQNRV
                     TFKDVAGVEEAKDEVEEIIAFLKDPKKFTRLGGRIPKGVLMMGSPGTGKTLLARAIAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLG
                     GGHDEREQTLNQLLVEMDGFESNDGVIIIAATNRPDVLDPALLRPGRFDRRIVVPRPD
                     LRGRIGILQVHTRKVPLAAGVDLEVIARGTPGFSGADLEFLVNEAALIAARRDKDKVE
                     AEDFEEAKDKVLMGAERRSMIISEKEKRTTAIHEAGHALVARFVGVEADPVHKVTIIP
                     RGRALGLTQQLPPEDRLSMTKEFAQNQIAIMMGGRVAEEIIFSQKTTGAGNDIERATE
                     LARSMVTEWGMSEEFGPLNFSPGKSEVFLGRDFSSNDRLSEDTARRIDAEIRRIVTTQ
                     YERATQILTERRKELEQVGEALLEYETLDGDDIDTLLRGGKITRPIAAIAKKQAAEAD
                     NERKRPASILPPISPVGKKDPTPEPA"
     gene            9996..10403
                     /locus_tag="VMZ53_30670"
     CDS             9996..10403
                     /locus_tag="VMZ53_30670"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HUS32920.1"
                     /translation="MAYDEELANRIRSLIGDEPNVTEKAMFGGLAFLVNGNMSVAASS
                     KGGLLLRVEPAQTMTHAKKPHAKPFDMRGKPMEGWLRVEPSGVTTDKGLAPWVKLSVA
                     FARSLPPKAKSKTAMKTKPTAKRPTGAAAKSRR"
     gene            10417..10722
                     /locus_tag="VMZ53_30675"
     CDS             10417..10722
                     /locus_tag="VMZ53_30675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS32921.1"
                     /translation="MSLIVRPARPEDWRAWRELRLRALAEAPDAFAQTLADVRDGDDA
                     AWQSWVRPRNDAIRLFAERDGVPIGQMVVIIAADDPRRANVYAMWVAPEARRTGLGR"
     gene            10771..11718
                     /gene="folP"
                     /locus_tag="VMZ53_30680"
     CDS             10771..11718
                     /gene="folP"
                     /locus_tag="VMZ53_30680"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HUS32922.1"
                     /translation="MELDVSDPMAGARGLYLACGFTPTGIREPLREGSGVQTERLRIR
                     LAPLVMGIVNVTPDSFSDGGQFLDPQRAVEHGWSLAEEGADILDVGGEATNPRATPVS
                     ADEELRRVLPVIEGLAKGGYLVSVDTTKAEVAWSAVKAGASIINDVSGGLFDARMAET
                     VVDLAASDDVTYIAGHLRGRTLAEVFAPEGRAAPPTWNEVAAELADRIRPLPASLRVW
                     VDPGIGFGKGADADGNVALLRHAGDIADRVRRPVVVGPSRKRFLRGVLGVQDASIPRN
                     VLDAASVLASLAGVRAGAHCVRVHNVALLRTHLTAYNKK"
BASE COUNT         2012 a         3815 c         3948 g         1962 t
ORIGIN      
        1 gcggccgtcg acgcgagccc ggagcctgcg ttcatgcgcg gcctcgccga ggcggcggcg
       61 tacaccggcg atggtcccgc cgcgctcgtg ttcgcgacga ccgcggctgc ttcgtccggc
      121 gatcctgcga tcgtctgggc tgcggttgcg gccgacctcg tcgcggcgaa tcagctcccc
      181 gattcgctca ccgcatcgcg caacgcactc gacctcgccg gccccgacgt gctgcccgcg
      241 gcgctcgaca cggcgattgc ctcgagccgc gcgctcggtc gcacgcaaca agccgacgcg
      301 cttctgctgc agcgcgcgca actcgcgccg cgcaaacgct ccgacgacac cgaggtgctc
      361 gctgcactcg cggcgcatcg cgaacggccg acggcgtcga ccgctgccaa cctgtgggtc
      421 gcgtcgcgcg cacttccacg cgacgtcgaa tcgcgcgccg ttctcctcga cgcgctcgac
      481 gccgacgacc ctcgacgcga gacgatccgc gacgagctcc tgctgctggc cggcgaccca
      541 gacccggccc gcgccctcgc ggccgcccac gcgttgcgtg attgagcgga cttagtcgct
      601 ggcaacaaaa tcagccacta tccaggcgct tgcgagatcg aatagcgttc ggtcgctgtg
      661 tctcgcgcta ttcggtcgcc gcttctcgct ccgctgcaac acgccgtgcc gctgcacctc
      721 gcggctccgc tgctcgcgct gttcgctgcg gccgcgtgca cgggctcgat cgacgatacg
      781 gcccgcgggg acgacaacag cgacggtccc gacgcgggca tcgacctcgg ccccggtgat
      841 cacgaagccg tcgcgcgcgc gaagctgtgg gtcgacgcca aggtcccgta ctgccaagcg
      901 cccaaccacc agcgcgatta cgacgaggcc tgcatgatga cgtgcacgcg ccccgacgtc
      961 gccgcgtggg atccatatcg cagcgactgc tcgggcctcg tctcgtggtc gtggggctta
     1021 ccaccgccag gccgcacgac gtccacgctc gcgccctaca acaccgacgt ctcgcactcg
     1081 atcgacgccg ccgacctaca gccgggcgac gcgatcaaca atgaccatca cacgatgatc
     1141 ttcgtcggct ggctcgagcc gatgacgaag gcccggttct acgaagagcc gggctgcagc
     1201 tcgtcgcagc cttacgcccg ccaatacgac gcgagcgtga tgatgatggc cggcaagacg
     1261 atcgtgctca catatcgcgg cacgtacacg gctatccggc agacgaagta gcgcctgcta
     1321 cttggagcgg cgcgcgaacc agtgcatgtg catcttgcgc aggaacgcat cgtcgaggcg
     1381 atgcgacgat ccactgccaa gaaccgagtc gacgccacac gacggacaca gtgcggtctg
     1441 atccgcatcc gtccacttct tgatcaacgt cggcgggaac gtgcgaaagc agaagaagca
     1501 gccgcagcgt ccgctccctt cgatttccgc acgatgctgc gtcgagtgct tgtgggcgtc
     1561 gacgaggatg tccataccag cgagcttagg gagcttgggg atgcttgttg cgaccatagt
     1621 gggccggacc tcgaatccgg tctctcggtg taccacgcct agatcaccgg ccctcgaaca
     1681 atcgcgacgc acgcgtgcgt gccaacggga tcgtgcgact tcgtcgcaag cgcactgaat
     1741 cgctcggtgg gcgctaacgg cgcggtggtg ggcgcttgcc ggtttgcttg taggcgaggt
     1801 agtccgagac gatcagcgcg tggtcgaaca cgagcggggc cgggagctcg tcgggtttgc
     1861 agacgacgca gcgtgaggcg tcgtcgccgc ctttaggagt gcctttcgcg cgcgcgatga
     1921 agaccgtcga gacggtgtgc ttgcgggagt cgcgctttgg gtccgagtag acgtggaaca
     1981 gctcgatgat ctcgacgtcg agctccgtct cttccttggc ctcgcggacc gcggcatcgg
     2041 cgatccattc gccttcatcg acgaagccgc cgggaagcgc gaagcctttg ggctcgttct
     2101 tgcgctcgat gaagacgagc gtgccgggcc tgccgtcgag ctcgatcagg atgtcgcacg
     2161 tcggggtggg attcttgtag tcgccgtcac cgctcattgg ttcgctcccg ataggaggct
     2221 ttgttcccag ctcgcgagct gctgctggag ctgtttggct tgtgtgtcgc tatcgacgac
     2281 gttcttgcgc tcttctggat cggccgcgag gtcgtagacc tcccagcgcg agtcactgat
     2341 gcggtggaag acgtggtgct tgccgtcggc ggagatcatc gagcgcgcgt cgtgcggcca
     2401 tgatttcgat ggcggcattt cggagaacgc gggctttgcg gggaggggcg ccccttcgag
     2461 cgcgccgacg aggctgcgac cgacccacga cttgggcggc gtcacgccga acagcgacgc
     2521 gatcgtcggc gatagatcga tcagctcgac gacgtcgctg acctcgcgcg gcttgttgtt
     2581 cggcacgcgg aacatcagcg gcacgtgcag cacctcgttg tagagcgtca tgccgtggaa
     2641 gaactgcttc tcgcccgcga acgtgtggat gccgaacgct tcaccgtgat cggacatcag
     2701 cacgaccgtc gtggagccgg cgagcttcag cgtgtcgagc tcgtcgagca gctcgccgat
     2761 cagtttgtcc tcgaacgcga tctcgtagtc gtacttctgc atcagcgacg cggtgccgcg
     2821 ctccgtgatc gccatgccgt cgtgctccat gtacgtcgag tgcggctcga acaggtgcac
     2881 gatcatcgcg aacttctgct tgccggccgc gagctcggcg agcttctttt tcgttttctc
     2941 gacgacgcgc ggacccgcga tatcgtgatt gctgcccgag atcgggagcg cgcccgtgtt
     3001 gtcccagagg tccgcacctt ggatcgcgtt ggtgcgcatc gggttgccgt cggtgttctt
     3061 cacgttgctg cacgtctcgg cgtacttctg gcgatcgcag aaatagaagt gcgacgactc
     3121 gccgatcgtc gtgaagccgg cgggcttgag gacctcgaag aggagctcgt tgtcgtcgag
     3181 cacgagcgga tagttcttct ccatcgcgtc gactttcagc ttcgtcggat actgcgacgc
     3241 gaggaacgac ggtaccgagc gcggcgtgtt cgatgcttgc gagtaagcgt tcttgaacac
     3301 gacggactgc ttcgcgaacg catcgatgcg cggcgtcagt gacttgccgt cgcgctgata
     3361 gccggtgaag ccgaggcgat cgtagcgaag cgtgtcgacg aagatgacga gcacgttgtc
     3421 gccgcctttg agcgtcggtg caggcgcgtt cttgcccgtc ggtaggtcgg gaagggttgg
     3481 cgctttctga ttgtcgaagc catcgcagtt ctcgtcgatg gtgttgtcgg gaacatcgat
     3541 gacgttggga tggatcgcct tgtcgcggtc gttgcagtcg gggccgccga agaatgcgga
     3601 gtagccgtcg tggtcgtggt ccgagagctt gcggaggacc ggaatgagcc gcggcccgac
     3661 gtacgatcga tcgagaaccg cgacttggtt cttctgcgat ggctcaccgc gtagcccgaa
     3721 caccgcgatc aggatcgcga acatcagccc gaccgaaacg aggatcacgc ggctcggaat
     3781 gcgctcgcgc acgcccgaca acggcccgta gaacacgatc gcgatcacga tcgcgaggat
     3841 cggcatcatc gccggcacga acagcgagcc gaggttgagc gcctgataat cgagcttctt
     3901 gaagatgacg aacagcccgg cgcacgcaac gagcaccgcg ccgaggaacg tcagcagcaa
     3961 gacgcgcgac agcgggccga tcgatgggac gacacccgtg atcttgcgcg tcacccggaa
     4021 gagcggcagc acggcgagtg cgagcacggg cacgagcgcg acgacgatca cgccgagcag
     4081 gagcccgcca acactctttc gctgcacatc gccgacgagc ccgacggaaa gcttcgcgac
     4141 gccgagaatg agcacacctg ccacgaccac tgccgcgatg aggatcgccg acaccgtgcg
     4201 atcgagctcg tcgtcctcgg acagccgccg aaacagccgg cgcacccagc cgctgcccca
     4261 cgtcgcgttg ccggcggcga tgacgagccc cgcgccgatc gcgaccggca gcgtgagcag
     4321 cgaccacagg ccgagcacct gcaagggtgc ggcaccggtg tgcacgacat cggcaatcgc
     4381 gagcacgaga ccggcgacga gcacgacacc ggctccggtc gcgagccccg atcgatacgg
     4441 cggaggagga gcgccctcgg acatctagcg aagcgttacc acagatatct gaggttcaag
     4501 gacgtgctgc cgcgggcttg gcgtggcgcg gcgtttgaac agatcgcgcg catgcgtctt
     4561 gctttgcttg cgtgcctttc gagcacgctc atttcgtgca cctacctcga agacgactgc
     4621 atggattgca acgaggagct cagctcggtt cgctacgacg tctcgtcagc gaccacgttc
     4681 aaccggctcg agatcaggct cgacggccgg cttgccgcgg agccgacgtt cttcgaagga
     4741 gtcaccgcgt atcactacga gcacgatgac gtcttcgcga cgtactacga cgagatcgcg
     4801 atcaccgcgt ttgtcgacga cgctccgatc gcggccggta gcgcagagcc cgcgtatcgc
     4861 cacgacggca tggttcacat cgccgaaggc gtcctcgcgt tgcagccgct gccggcgttc
     4921 gctccgcgct agctgcgctg cacttcgacg atcacacctt cgacgaaccg cggatcgaca
     4981 tcgccttctt tgatgccgcc gaggatgcgc tcgagatcgt cgagccagct gtcgatcaac
     5041 cggccgagca gcggaccgga tgcagcgagc tgcgcagcac cgaggcgcgc gttgatcgca
     5101 gcgtgtcctg cgcggcgcgc gatgtcggtg agcttgcctg aggcatcgaa ggcggtcttg
     5161 cggagctgcg tcagctcggc ttgcgagagc gcgtcgccgc ggagctcttc gccgccgacc
     5221 agcgagcgcg cgagcgcggt gcgaatgtga tcgtcgagct cgggccgcgg ctcgggcgct
     5281 tggcccatcg cgaggacgtc gacgccgagg ccttcgacga gcgcgatccg aatcgtcagc
     5341 ccggtgagaa tctcgccggc tcggcgcaag tccgcttgcg actcgaacgg gcgcaggccc
     5401 ggcatcgctg gctcgtcggc ggcgcgcgag aacagagggc gcggcgagca gaggcccgcg
     5461 acgagatcct tcgccggaga gccggcggtg ggcgagcgag gtgcgagcgc ggtcgcgagc
     5521 ttcgcgagct tgtgcgtgac cgtgtatccg acgcggaaca gacggccgag cgagatcgac
     5581 tgcagcgctt gcttggcgcg ctccagatcg ccgcgtgcga cgacgtcgag gccgagtgaa
     5641 agcgtggccg tcgcatagag cgcaccacgg cgcatcactt cgctctggcc cggcttcgcg
     5701 cgacctgcag cgagcacctt gttgacgagc accatgagcg agccctcgag acgttcggcc
     5761 tcgcggtcgt cgtcgattgc ggccatcgcg cgagcgagga agctgcggcc gatgacttct
     5821 tcgacgatga cgacgggcag atgcgagctc gcatcttcga taggccgatc ctgcgagccc
     5881 tcgccgatgt gcacttgatc ggccggcagc gggcggaaca gatcgagcgc ttcgtagaag
     5941 tcgacgtagc cgaaatcggc gagccggccc gagcgccagc gatacgccat ctcttcgagc
     6001 tcggcgggcg gctcgctgcg cgcagccatg atcgtatgac gcgcgagctg cggatcggcg
     6061 cggtagaggt cctcgacgag ctgctgcgtg aggcgctgcg tgtcgtcgtc gcctttgagc
     6121 tcgagcaaga agaaccgatc cggcgtcgtc atgatcggct cgtcgttgtc ctcttcgggc
     6181 ccctcgttca tcgtcgtgtc gtagacgagg acctgcttct gcaggatcaa cgtgcggagc
     6241 tctgcgtcga gcgcgcccca gacctcgccg actttctcga agccgacttc gatcagtgcc
     6301 gcgagccacg gcttcagcga gacgacctcg agctgatcct tgttccagcc atcgagatcg
     6361 aagcagcctt ggatctgcga cggcgtcgcg tactcgatca gcgcttggcc atcttcgaag
     6421 ccaacctcgt gcacgagctc gtagatctcg ctcgcggaca gcgcggcgat cgcggcgggc
     6481 gcatcatcgg cggacagcaa cgcgtcgatc cgccggtaac cacgcgggcc ggcgagatgc
     6541 gcgcggagtc gggccagggg accgctcgga ggagggacgt cgcgttcgct catgtgtaaa
     6601 ggttggctag tgggtaccac gccgcgcgat ccagtgagca cgctgcgtgc ggcggtcaag
     6661 ctgtggatcg accagctagg cgccggcctg tacggcctgg cctgttcagg tggcgcggat
     6721 tcgatggcgc tcgccgacgc cgcgtgtgcc gtcgccggcc gcgagaatgt cgtgctggtg
     6781 cacatcgatc acgggcttcg cgacgacagc gcgacggtcg ccagcgagct cgccgcgtgg
     6841 gcgaaagggc agggcatcac cgcggtcgtg cgtcgtgtcg acgtgcgcgg cgagtcggtg
     6901 gaagcggcgg cgcgcgaggc gagataccgc gcgttcggcc agctgcacga agagctcggg
     6961 ctgtctcgca tcctgttggc tcacacggcg cgcgatcagg cggagaccgt cctcatgcgg
     7021 atgctgcgcg gcacggggcc cgccggtctc gcagcgatgg cggcgcagcg cgatgtctac
     7081 gtgcgtccac tgctcgagat ctcacgcgag atcgtcgacg actacatcgc cgcgcgtgga
     7141 ttgccgacgt ggcgtgatcc gatgaacgag gacccagcgt tcacgcgcgt gcgcctgcgg
     7201 accgtcgtgc tccctgcgct gcgcgcagaa aacccagcga tcgatgatgc tctcgtacga
     7261 ctcgcgcgtg cggccggcga atggcgcgat gcgatcgatg cgctcgccga accctttgcg
     7321 acatttccga tcgattgtga ggagctcgcg acgcagctgc ctgcgatccg aaaacgcgct
     7381 gtcgcgttgg cgctcgaacg cgcgcggctg ggatacgaag ctgcgcacct cgatgcgatc
     7441 gatgcgctgg tgatggcgcc gacagcggga gagcattcga tcgatgtgcc cggcgcgcgc
     7501 ctcgttcgaa gctacgagac actcgaaatt cggtccgacc aaatttcgga aattcagtcc
     7561 gacctaattt ccggcgacta cgaagtgcgc gtctggcggc ccggagatcg gatgaagccg
     7621 gctcgtctca aaggccgctc ccgcaagctg tccgacctct acatcgatgc gaaggttcca
     7681 cgcgcgattc gcgcagcatc acgcgttgtc atccgtcgtg cagatgcagt gatcatctat
     7741 gccgagcatc tcgggatttc ggtcgacgcc cgcccgaatg attcggagtt tttgggatca
     7801 attagctttg caagccagct caaaggcccc atgctaagca acggcagggg ctaactgcgc
     7861 gagacatgcg acaatctcac aagacaatcc tgatctgggc gatcctgatt cttatgttcg
     7921 tgagcatcta ttcgatgttc acggactcct cgtccaagga gaaggaggtc gacgtcacga
     7981 ccttccgcgc cgacttgttg aacaaggaga aggcgaagga gatcgaagag atcgcgatcg
     8041 agccgggtgg ccatgacaac gcccgctacg tgatcaagaa gtcgaactcc agcgtccgcg
     8101 aagtcgtgca cgccgagttt cccggcacga tcacgaaaga gatctacgac gcgggcatca
     8161 agtaccgcgt aaaggccaag gacgactcga gcatctggcc gcaggtgctc gtgtggtggc
     8221 tgccgatgct gctcttggtc ggcatcttct tcttgtttat gcgccagctg cagagcggcg
     8281 gtggcaaggc gatgtcgttc ggcaagtcga aggcgaagct gctctccgat caccagaacc
     8341 gcgtgacgtt caaagacgtc gccggcgtgg aagaagccaa ggacgaggtc gaggaaatca
     8401 tcgcgttcct caaggacccg aagaagttca cgcgcctcgg cggccgtatc ccgaagggcg
     8461 tcctgatgat gggctcgccg ggtaccggca agacgctgct cgctcgcgcc atcgcgggtg
     8521 aagcaggcgt gccgttcttc tcgatctccg gttcggactt cgtcgagatg ttcgtcggcg
     8581 tcggcgcgag ccgcgtccgt gacctcttcg agcaaggcaa gaagaacgcg ccgtgcatca
     8641 tcttcatcga tgagatcgac gctgtcggcc gtcaccgtgg cgcaggcctc ggcggtggtc
     8701 acgacgagcg cgagcagacg ctgaaccagc tcctcgtcga gatggacggc ttcgagagca
     8761 acgacggcgt catcatcatc gccgcgacca accgccccga cgttctcgat ccagcgctgc
     8821 tgcgcccggg tcgcttcgac cgccgcatcg tcgttccgcg cccggatctc cgcggccgta
     8881 tcggcatcct ccaggtccac acgcgcaagg tgccgctcgc ggcaggcgtc gatctggaag
     8941 tcatcgcgcg cggtacgccc ggcttctccg gcgcggacct cgagttcctc gtcaacgaag
     9001 cggctctcat cgccgcccgt cgcgacaagg acaaggtcga ggccgaagac ttcgaagaag
     9061 cgaaggacaa ggtcctcatg ggcgccgagc ggcgctcgat gatcatctcc gagaaggaga
     9121 agcggacgac ggccatccac gaggcaggcc acgcgctcgt cgcgcggttt gtcggcgtcg
     9181 aggccgaccc ggttcacaag gtgacgatca tcccgcgtgg ccgcgcactg ggcctcaccc
     9241 agcagctgcc gcccgaagat cgcctctcga tgacgaagga gttcgcacag aaccagatcg
     9301 ccatcatgat gggcggtcgc gtcgcggaag agatcatctt cagccagaag acgaccggtg
     9361 ccggcaacga catcgagcgc gcgaccgagc ttgcccgcag catggtcacc gagtggggca
     9421 tgagcgaaga gttcggcccg ctgaacttct cgccgggcaa gagcgaagtc ttcctcggcc
     9481 gcgacttctc gagcaacgac cgcctgtcgg aagacacggc gcgtcgcatc gacgccgaaa
     9541 tccgccgcat cgtcacgacg cagtacgagc gcgccacgca gatcctcacc gagcgccgca
     9601 aggagctcga gcaggtcggt gaagcgctgc tcgagtacga gacgctcgac ggcgacgata
     9661 tcgatacgct gctgcgcggc ggcaagatca cgcgcccgat cgcggcgatc gcaaagaagc
     9721 aggccgccga agccgacaac gagcgtaagc gtcccgcctc gatccttcct ccgatcagcc
     9781 cggtcggtaa gaaggacccg acgcccgaac cggcgtaacg ccagtccgcg ccggccggct
     9841 gcgcgcgtca tcgaagaggc cccacgcggg gcctcgtttc gtttcggcgc gccgcccgcg
     9901 gcgttcacgg tctcgtcaca gtagacaatc gcgccgccgt tcgagccgtc gcgcgaggga
     9961 tgcctcgacc gtcttggttc gggcatcatc ggcgcatggc ctacgacgag gagctcgcga
    10021 accgcatccg gtcgctgatc ggcgacgagc cgaacgtcac ggagaaggcg atgttcggcg
    10081 ggctcgcgtt tctcgtgaac ggcaacatgt cggtcgcggc gagcagcaag ggcggcctgc
    10141 tcctgcgcgt cgagcctgcg cagacgatga cgcacgcgaa gaagccgcac gccaagccgt
    10201 tcgacatgcg cggcaagccg atggaagggt ggctgcgcgt cgagccgtcg ggcgtcacga
    10261 cggacaaggg cctcgctccg tgggtgaagc tcagcgtcgc gttcgcgcgc tcgctcccgc
    10321 cgaaagcgaa gagcaagaca gcgatgaaga cgaagcccac ggcaaaacgc cctacgggcg
    10381 ctgcggcgaa gtcgcgccga taacgtatcg tcgggtgtgt cgctcatcgt gcggcctgcg
    10441 cggcccgagg actggcgtgc atggcgcgag ctgaggctgc gtgcgctcgc ggaagcgccc
    10501 gatgcgttcg cgcagacact cgccgatgtt cgcgatggcg acgacgcggc gtggcagtcg
    10561 tgggttcggc cgcgcaacga tgcgattcgc ctgttcgccg agcgcgatgg cgtgcccatc
    10621 gggcagatgg tcgtcatcat cgcggcagac gatccgcggc gcgcgaacgt gtatgcgatg
    10681 tgggtggcgc ccgaggcgcg gcggacaggc ctcgggagat agctggtcga ggcggggctc
    10741 gcatgggcgc ggcagcgggg cgccctcgac gtggagctcg acgtcagcga tccgatggcc
    10801 ggcgcgcgtg gcctctatct cgcgtgcggc ttcacgccga ccggcatccg agagccgctg
    10861 cgcgaaggct cgggcgtgca gaccgaacgc ctgcgcatcc gtctagcgcc gctcgtcatg
    10921 gggatcgtca atgtgacgcc tgactcgttc tcggatggcg gccagttcct cgacccgcag
    10981 cgggcggtcg agcacggctg gagtctggcg gaggagggcg ctgacatcct cgacgtcggt
    11041 ggcgaggcca ccaatccgcg tgcgacgccg gtctccgccg acgaagagct gcggcgcgtg
    11101 ctcccggtga tcgaggggct cgcgaagggc ggctacctgg tgtcggtcga tacgacgaag
    11161 gccgaggtcg cgtggtccgc ggtgaaggcg ggagccagca tcatcaacga cgtctcgggc
    11221 ggcctgttcg atgcgcggat ggcagagacc gtcgtcgacc tcgcggcgag cgacgacgtc
    11281 acctatatcg ccgggcactt gcgcggccgg acgctcgccg aggtgttcgc gcccgagggc
    11341 agggcagctc caccgacgtg gaacgaggtc gctgccgagc tcgccgaccg tatccgcccg
    11401 ctacccgcgt cgcttcgcgt gtgggtcgac cccggcattg gctttggcaa gggcgccgac
    11461 gccgacggca atgtcgcgct acttcgtcat gccggtgaca tcgccgaccg cgtgcgaaga
    11521 cccgtcgtgg tcgggccgag ccgcaaacga ttcctacgcg gagtgcttgg tgtgcaggac
    11581 gcgtccatcc cacgcaacgt gctcgatgcc gcgtccgtcc tcgcgtcact cgccggcgtg
    11641 cgcgcgggcg cgcattgcgt tcgagtccac aacgttgcct tgctccgcac gcacctgacc
    11701 gcgtacaaca agaagtagag cggtgactgc gtcgatc
//
LOCUS       DATLBZ010000624         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8750763, whole genome shotgun sequence.
ACCESSION   DATLBZ010000624 DATLBZ010000000
VERSION     DATLBZ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8750763"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VMZ53_30685"
     CDS             <1..1234
                     /locus_tag="VMZ53_30685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32923.1"
                     /translation="VTPVDALTFKFNFEATVAGQNSFLSLLPGTSAGGGKKIDLKMGK
                     VTNEELLAGGWTGRYDVHSWDPNKVDAATVETLPMTVTQAPRSANAYLDYNRLYADGK
                     LEVGAQFGWDYNAGRADLVNAEQLYDELVDIGFKSPVGSFRDLKLDSPALTRAGTYNG
                     KSVAISVKLVHPGMVADAAANAVDLRNALLDLLKTKEVILFNGHAGVSGRLLPADFRS
                     TSAGNILPAEYPTLQLLDGYQVFLIEGCQTYARFTDGFRQNPKKKGPAGELVNMDIVT
                     STSYTWTSQGAESMEAILWPLVGTSATSNIKPTTWDDILRKMNAPPNSTAFMGVNGID
                     GDPHAHPYARADKLGLSCATNAQCGGEGNYCLKQANTTAKVCGSVCIDDAGCPSTHRC
                     SSVASSGTLASTKACVKR"
     gene            1244..1825
                     /locus_tag="VMZ53_30690"
     CDS             1244..1825
                     /locus_tag="VMZ53_30690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32924.1"
                     /translation="MKLLVACVLVAGCASAGSGDRPDASGGGKQDGSIIKMDAALQID
                     APSMSTGCTSSQQCTSAMMLGNVSGDSGNAKLTAQGYQSAWYRVRVTEDNSTVVPLTL
                     RMAARVTSPPGMTFHVATYVNTGSNAVECATTVGSPTTAGNTEEIKIEWGEMGTFSNG
                     SDDGRDVSIEVKAPASGCSSSLMWQLEVEGNWL"
     gene            1827..>2174
                     /locus_tag="VMZ53_30695"
     CDS             1827..>2174
                     /locus_tag="VMZ53_30695"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HUS32925.1"
                     /translation="MNCPRCETPMQTRPLAETPLHECAACGGLFLAHGTIERILEDRG
                     GTLVANELLASLPRVPTKPTPPPGQRMYVSCPTCGTLMNRKLFATGAGIILDVCRADG
                     AFFDAGELPAIVEF"
BASE COUNT          407 a          711 c          714 g          342 t
ORIGIN      
        1 ggtcacgccg gtcgacgcgc tcacgttcaa gttcaacttc gaagcgaccg tcgcgggaca
       61 gaacagcttc ctctcgctcc tgccgggcac ctcggcgggc ggcggcaaga agatcgatct
      121 caagatgggc aaggtcacga acgaggagct cctcgcgggt ggctggaccg gccgctacga
      181 cgtccactcg tgggacccga acaaggtcga cgcagcgacc gtcgagaccc tgccgatgac
      241 ggtcacgcag gcgccgcgct cggcgaacgc gtacctcgac tacaaccgcc tctacgccga
      301 cggtaagctc gaagtcggcg cgcagttcgg atgggactac aacgcgggtc gcgctgacct
      361 cgtgaatgcc gagcagctct acgacgagct cgtcgacatc ggcttcaagt cgccggtcgg
      421 ctcgttccgc gatctcaagc tcgactcgcc ggcgctgacg cgcgcgggca cgtacaacgg
      481 caagagcgtc gcgatctccg tcaagctcgt gcaccccggc atggtcgcgg acgccgctgc
      541 aaacgcggtc gaccttcgca acgcgctgct cgatctgttg aagacgaagg aagtgatcct
      601 gttcaacggt cacgcgggtg tgtccggacg cttgctcccc gccgacttcc gctccacgag
      661 cgccggcaac atcctgccgg cggagtaccc gacgctgcag ctcctcgacg gctatcaggt
      721 gttcttgatc gaaggctgcc agacatacgc gcggttcacc gacggcttcc gccagaaccc
      781 gaagaagaag ggccctgccg gcgagctcgt caacatggat atcgtgacgt cgacctcgta
      841 cacgtggacg tcgcagggcg ccgagtcgat ggaagcgatc ctgtggccgc tcgtcggaac
      901 ctctgccacg tcgaacatca agccgaccac gtgggacgac atcctgcgca agatgaacgc
      961 gccgccgaac tcgaccgcct tcatgggcgt caacggcatc gatggcgacc ctcacgcgca
     1021 tccgtacgcg cgggcggaca agctcggctt atcttgtgcc acgaacgcgc agtgcggcgg
     1081 cgagggtaac tactgtctca agcaagcgaa tacgaccgcc aaggtctgcg gctcggtctg
     1141 catcgacgac gccggctgcc cgtcgacgca tcgctgctcg tcggtcgcct cgagcggcac
     1201 tcttgcctcc acgaaggcgt gcgttaagcg gtagaatgcc cgggtgaagt tgctcgtcgc
     1261 ctgcgtgctc gtcgctggat gtgcatccgc gggcagcggt gatcggcccg atgcgagcgg
     1321 cggcggcaag caagacggca gcatcatcaa gatggatgcc gcgctgcaga tcgatgcgcc
     1381 ctcgatgagc acgggctgca cgtcgagcca gcagtgcacg agcgcgatga tgctcggcaa
     1441 tgtaagcggc gattccggca atgcgaagct gacggcgcag ggctatcaga gcgcgtggta
     1501 ccgcgtgcgc gtcaccgagg acaacagcac cgtcgtgccg ctcacgttgc gaatggccgc
     1561 gcgcgtgacg tcgccacccg gcatgacgtt tcacgtcgcg acgtatgtga acacgggcag
     1621 caatgccgtc gagtgcgcga cgaccgttgg ctcaccaacg accgcgggca acaccgaaga
     1681 gatcaagatc gagtggggcg agatgggcac gttctcgaac ggatccgacg acggtcgcga
     1741 tgtctcgatc gaagtgaagg cgccggcgag cggctgcagc tcgtcgctca tgtggcagct
     1801 cgaagtcgaa ggcaactggc tgtagcgtga actgcccgcg ctgcgaaacg ccgatgcaaa
     1861 cgcggcctct cgcggagacg ccgctgcacg agtgcgccgc atgcggaggt ctgttcctcg
     1921 cgcacggtac gatcgagcgc atcctcgaag atcgcggcgg gacgctcgtg gcgaacgagc
     1981 tgctcgcgag cttgccgcgt gtgccgacga agccaacgcc accgcccggc caacgcatgt
     2041 acgtgagctg cccgacgtgc ggcacgctga tgaatcgcaa gctgttcgcg acgggagcgg
     2101 gcatcatcct cgatgtctgc agagccgacg gcgcgttctt cgatgcgggc gagctacccg
     2161 cgatcgtcga gttc
//
LOCUS       DATLBZ010000625         5969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10151512, whole genome shotgun sequence.
ACCESSION   DATLBZ010000625 DATLBZ010000000
VERSION     DATLBZ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10151512"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VMZ53_30700"
     CDS             <1..417
                     /locus_tag="VMZ53_30700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HUS32926.1"
                     /translation="FAHNLETVPRLSRQVRVQASYPRSYKILEHARKRGAVTKTGLML
                     GLGETLDEVRDVIRELAGLGVDIVTLGQYLSPSKQHLAIERYVHPTEFAELEQFALGA
                     GITHVVAGPLVRSSYHADGQAELVRNLRRDAAKSAQ"
     gene            complement(406..1152)
                     /locus_tag="VMZ53_30705"
     CDS             complement(406..1152)
                     /locus_tag="VMZ53_30705"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HUS32927.1"
                     /translation="MSPFVVALLTLAAFCAGTVDAIAGGGGLITVPALLTAGLPPVEA
                     LGTNKGQSAWGSAAALTAFWRAGRVDKKQAAFTFPLGLAGSFCGALVVSAIDKQLLRP
                     IVIAMLIGAAVLLVVRKPMREQDAPRARPLVAAGLALAIGAYDGFFGPGTGTFLIVGF
                     VALCGRSLLHASADAKVVNFASNCAAVLVLGYHGFIHWDIALPMAGGQLLGGMIGAHF
                     AMRGGDRIVRILVLVVSGALIVKLLVDLLR"
     gene            1180..2136
                     /locus_tag="VMZ53_30710"
     CDS             1180..2136
                     /locus_tag="VMZ53_30710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUS32928.1"
                     /translation="MEVTVVGAGIIGLTTALTLQEHGHRVRVVAEAPWSANVSGIAGA
                     VWFPYKVGPPARVNVWAARTRRWLEQIARTSPDAGVDVLTGYEITLEPHGELQRPWWA
                     GEPTAELQSAHIEDAIAVERVPAPVEDAPHAWKFIAPRAQPSLFLPFLEAALSRPVER
                     RRVTDLAAEPGDVVVNCTGIAARELCNDDKLVPLLGQVVIVERGAADLTTTITDDRDA
                     DDFFYIIPRRDELVLGGCSIPFPPGDTPEIDPEITARILAHAKRLGISIGQLRRERVG
                     LRPYRAEVRLEREGRVIHNYGHGGAGFTLCRGCAEDVATLVR"
     gene            complement(2137..3549)
                     /locus_tag="VMZ53_30715"
     CDS             complement(2137..3549)
                     /locus_tag="VMZ53_30715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS32929.1"
                     /translation="MSDDLSARPRTNTRAKAEAPPGMALSGEEGRKHIGRYEVVKTIG
                     KGAMGVVYKARDPLLDRTVAVKTIMSPQQQGRRVRSAFLERFQREAKAAAKMSHPAIV
                     TIFDVGVDEDSGAPFMVLEYLPGESLADRLDRVRLPLARCVQIGLDLSSALSFAHRQR
                     IVHRDVKPANVLHAGDNRWKLADFGIARLPDSDLTQVGIFMGTPGYSPPEAIKEGRYT
                     PQADVFAWGAVMYELMSGRIPYEGPDTKTTNGYVMQGNAPPPTRHDASIPEPLATVTM
                     TALQPSDKARYRDASEAEQALREAWERCLTQGLVHPAVLAMDEIPHDKAPLRMHEPRG
                     GNNRAAPMANNAPLNLGDSQEMTAINAKPPQKGFVIESTKARPGDDDPTKLMVREEGK
                     GKGDLTKQGVGPSGRSPAITNDTTKEAAVRDSVADTKADPSVVRRMGGSSKLPWIIVA
                     VVLVLAAAAVGLYFAGVLKL"
     gene            complement(3598..4536)
                     /locus_tag="VMZ53_30720"
     CDS             complement(3598..4536)
                     /locus_tag="VMZ53_30720"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF018165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HUS32930.1"
                     /translation="MSERRPLVVSSEPARQPDVADQLLEAYLDPSAAEMQHIGSYELP
                     SDAQVEHVIDMCRALLFPGYAGPDVARLEKAQLRELVRLRLEELKVSLHRQVYRALHH
                     KRQMKLGKNELECADCTRNAHDITERFITHLPELRARVRLDLRAAYESDPAATGIDEI
                     LLTYPGAYAVTVYRIAHCLLGQGAVIIPRMMTELAHRRTGIDIHPGATIGNALFIDHG
                     TGVVIGETTKIGDRVRIYQGVTLGALTIPQGEARPVPGSQRHPTIEDDVVIYANATIL
                     GGETVIGKGAVIGGNAFITTSVAAGTKISGSSRTQK"
     gene            complement(4547..5416)
                     /locus_tag="VMZ53_30725"
     CDS             complement(4547..5416)
                     /locus_tag="VMZ53_30725"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HUS32931.1"
                     /translation="MNVELVAIQLAVDAEAIASADAYRRHLEAAAATALDSGGSDARL
                     VVFPEVAGHLALYALAPPRARKAKTLASALAQAAVRRPLDVLRGVVSTRLLDARHAVL
                     AAIAPDGERWWKALFGPLARQYNAYVVAGSFLRLHADGSITNSSHLFGPDGSLLATTD
                     KVNLLPGVEDGAPGGLALHRGDSHVPIVQTPFGKLATLIGYDACREPQTTQERFVALG
                     PRLTERGGVDIVANPSANPLGKETPGDELARYVVTAHLVCKVLDLAFEGSSEIRGAET
                     SRRGGHVVARVSC"
     gene            complement(5413..>5969)
                     /locus_tag="VMZ53_30730"
     CDS             complement(5413..>5969)
                     /locus_tag="VMZ53_30730"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HUS32932.1"
                     /translation="LGSGWGVLFSHPKDFTPVCTTELGRVSALKPEFDKRGVKVLGLS
                     VDPLDDHKRWVGDIKETQGHALNFPLIADPKREVANLYGMIHPNANDTLTVRSVFVIG
                     PDKKIKLSITYPASTGRNFDEILRVIDSLQLTAKHKVATPVDWKQGGDVIIVPSVSDD
                     DAKKQFPNGWKTLKPYLRVVPQPK"
BASE COUNT          979 a         2032 c         1986 g          972 t
ORIGIN      
        1 ttcgcgcaca acctcgagac ggtgccgcgc ctctcgcgtc aggtgcgcgt ccaagcgagc
       61 taccctcgca gctacaagat cctcgagcac gcccgcaagc gcggcgctgt cacaaagacc
      121 ggcttgatgc tcggcctggg cgagacgctc gacgaagtgc gcgacgtcat ccgcgagctc
      181 gcgggcctcg gcgtcgatat cgtgacgctc ggccagtacc tctcgccgag caagcagcac
      241 ctcgcgatcg agcgctacgt ccatccgacg gagttcgccg agctcgagca gttcgcgctc
      301 ggtgcaggca tcacgcacgt ggttgccggg ccgctcgtgc ggtcgtcgta tcacgccgat
      361 ggccaagcgg agctcgtccg caatctgcgc cgcgacgctg cgaagtcagc gcaatagatc
      421 gacgagcagc ttcacgatca gcgcgcccga tacgacgagc acgaggattc gcacgatccg
      481 atcgccgccg cgcatggcga agtgcgcgcc gatcatcccg ccgagcagct gaccgcccgc
      541 catcggcagc gcgatgtccc agtgaatgaa gccgtggtag ccgagcacga gcacggcagc
      601 gcagttcgac gcgaagttga cgaccttcgc atcggcggac gcgtgcagca ggctgcggcc
      661 gcagagcgcg acgaagccga cgatcaagaa cgtgccggtg cccggaccga agaagccatc
      721 gtacgcgccg atcgcgagcg cgagaccagc ggcgacgagt ggacgtgcac gcggcgcgtc
      781 ttgctcgcgc atcggcttgc ggacgacgag caggacggcg gcgccgatca gcatcgcgat
      841 cacgatcggg cgaagcagct gcttatcgat cgcgctcacg acgagcgcgc cgcagaacga
      901 gccggcaagg cctagcggga acgtgaaggc agcctgcttc ttgtcgacgc gacctgcgcg
      961 ccagaatgcg gtcagcgcgg cggccgagcc ccacgcggat tggcccttgt tcgtgccaag
     1021 cgcctcgacg ggcggcaggc ccgccgtcag cagcgccggc accgtgatga ggccgccacc
     1081 ccctgcgatc gcgtcgaccg tgccagcgca gaacgccgcc agcgtcagga gcgcgaccac
     1141 gaaaggtgac acgcacgaac gttacactat gctttgcaca tggaagtaac ggtcgtcggc
     1201 gccggcatca tcgggctgac gactgcgctg acgctgcagg agcacggaca tcgcgtgcgc
     1261 gtcgttgccg aggcgccgtg gtcggcgaac gtatcgggca tcgccggcgc cgtgtggttc
     1321 ccgtacaaag tcggcccccc agcgcgcgtg aatgtttggg cggcgcgaac gcgccgttgg
     1381 ctcgagcaga tagcgaggac gagccccgac gcgggcgtcg acgtgctcac cggctacgag
     1441 atcacgctcg agcctcacgg cgagctgcaa cgaccgtggt gggccggcga gccgacggcg
     1501 gaattgcagt ccgctcatat cgaagacgcg atcgcggtcg agcgagtgcc cgcgcccgtc
     1561 gaagacgcac cgcacgcgtg gaagttcatc gcgccgcgcg cgcagccgtc gctgtttcta
     1621 ccgttcctcg aagcggcgct ctcgcgaccg gtcgaacgcc ggcgcgtgac agacctggcc
     1681 gcagaacccg gcgatgtcgt cgtcaactgc acgggcatcg cggcgcgcga gctgtgcaac
     1741 gacgacaagc tcgtaccgct gctcggccaa gtcgtcatcg tcgagcgcgg cgccgcggat
     1801 ctcacgacga cgatcaccga cgaccgcgac gcggacgact tcttctacat catcccgcgt
     1861 cgcgacgagc tcgtgcttgg cggctgctcg atcccgttcc cgcccggcga cacgcccgag
     1921 atcgatcccg aaatcacggc gcgcatcttg gcgcacgcga agcgcctcgg catctccatc
     1981 ggacagctgc gccgcgagcg cgtcggcttg cgcccgtatc gcgccgaggt ccggctcgag
     2041 cgcgaaggcc gcgtgattca caactacggc cacggcggtg cgggcttcac gctatgccgc
     2101 ggctgcgccg aagacgtcgc gacgctcgtg cgctgactac agcttcagca cgccggcgaa
     2161 gtacaggccg acggctgccg cagcgagcac gagcaccacc gcgacgatga tccacggcag
     2221 cttgctcgac ccgcccatcc gacgcacgac cgatggatcc gccttcgtat cagcgacgct
     2281 gtcgcgaacg gcggcttcct tcgtcgtgtc attcgtgatc gccggcgagc ggccactcgg
     2341 accgacgcct tgcttcgtca gatcgccttt gcccttgccc tcttcgcgaa ccatcagctt
     2401 cgtcggatcg tcgtcgccgg ggcgggcctt ggtgctctcg atcacgaagc ccttttgcgg
     2461 cggcttcgcg ttgatcgcgg tcatctcctg cgagtcgccg agattgagcg gcgcgttgtt
     2521 cgccatcggt gcagcgcggt tgttgccacc gcgcggctcg tgcatgcgca gcggcgcctt
     2581 gtcgtgcgga atctcgtcca tcgcgagcac ggcgggatgc acgagacctt gcgtcaggca
     2641 gcgctcccac gcttcgcgca gcgcttgctc tgcttcggac gcatcgcggt agcgagcctt
     2701 gtcgctgggc tgcagcgcgg tcatcgttac cgtcgcgagc ggctcgggaa tcgacgcatc
     2761 gtgacgcgtc ggcggcggcg cattgccttg catcacgtag ccgttcgtcg tcttcgtgtc
     2821 cggaccctcg tacggaatgc ggcccgacat cagctcgtac atgacggcgc cccacgcgaa
     2881 cacgtcggcc tgcggcgtgt agcggccttc tttgatcgcc tcgggcggcg agtagcccgg
     2941 cgtgcccatg aagatgccga cctgcgtgag gtcgctgtcg ggcagacgcg cgatgccgaa
     3001 atcggccagc ttccagcgat tgtcgccggc gtgcagcacg ttcgccggct tcacgtcgcg
     3061 atgcacgatg cgctggcgat gcgcgaacga cagcgcgctc gacagatcca aaccgatttg
     3121 cacgcagcgc gcgagcggca ggcgcacgcg atcgaggcgg tccgcgagcg attcgcccgg
     3181 cagatactcg agcaccatga acggcgcgcc ggaatcttcg tcgacgccga catcgaagat
     3241 ggtgacgatc gccggatgtg acatcttcgc ggcggccttc gcctcgcgct gaaagcgctc
     3301 gaggaatgcg gaccgcacgc ggcggccctg ctgctgcggg ctcatgatcg tcttcaccgc
     3361 gacggtgcga tcgagcagcg gatcccgcgc cttgtagacg acgcccatcg cgcctttgcc
     3421 gatcgtcttc acgacctcat agcgcccgat gtgcttgcgc ccttcctcgc cggagagcgc
     3481 catccctggc ggcgcctcgg ccttggcgcg cgtattcgtg cgcgggcgag ctgataggtc
     3541 gtcgctcatc aacccggtcc ggagcttaac acgcctcggc gaaagcccag agaaacccta
     3601 cttctgcgtc cgtgacgagc ctgaaatctt cgtgcccgcg gctaccgacg tcgtgatgaa
     3661 cgcgttgccg ccgatcacgg cgcctttgcc gatgaccgtc tcgccaccga ggatggtcgc
     3721 gttggcatag atcaccacgt cgtcctcgat ggttgggtgg cgctgggagc cggggaccgg
     3781 gcgggcttcg ccctgcggaa tcgtgagcgc gccgagcgta acgccctgat agatgcgcac
     3841 gcggtcaccg attttcgtcg tctcgccgat cacgacgccg gtgccgtggt cgatgaacag
     3901 cgcattgccg atggtcgcac ccgggtggat gtcgatgccc gtccggcgat gcgccagctc
     3961 ggtcatcatg cgcgggatga tgacggcgcc ctgaccaagc aggcagtgcg cgatccgata
     4021 gaccgtaact gcatatgcac ctggatatgt aagcaggatc tcgtcgatgc ccgtcgccgc
     4081 gggatccgac tcgtaggccg cgcgcagatc gaggcgcacg cgggcgcgca gctccggcag
     4141 atgcgtgatg aagcgctccg tgatgtcgtg cgcgttgcgc gtgcagtcgg cgcactcgag
     4201 ctcgttcttg ccgagcttca tctgccgctt gtgatgaagc gcgcgataga cctggcgatg
     4261 cagcgagacc ttcagctcct cgagccgcag ccgaacgagc tcgcgcagct gcgccttctc
     4321 gagacgcgcg acgtcggggc cggcgtaacc ggggaacaag agcgcgcgac acatgtcgat
     4381 cacgtgctcg acctgcgcgt cactcggaag ctcgtagctg ccgatgtgct gcatctccgc
     4441 cgcgctcgga tcgaggtagg cctcgagcag ctggtccgcg acgtcgggtt gccgggcagg
     4501 ctccgagctc acgacgagcg ggcggcgttc agacatctct cgatgtctag cacgagaccc
     4561 gggcgaccac gtgtcctcct cgacgggagg tctctgcgcc gcgaatctcc gaggagccct
     4621 cgaacgcgag gtccagcacc ttgcagacga gatgcgccgt gacgacgtag cgcgcgagct
     4681 catcgccagg tgtctctttg ccgagcggat tcgccgacgg gttggcgacg atatcgacgc
     4741 ctcccctctc cgtcagccgc ggacccagcg ccacaaatcg ctcctgggtc gtctgcggct
     4801 cgcgacacgc gtcgtagccg atcagcgtcg ctagcttgcc gaacggcgtc tgcacgatcg
     4861 gcacgtgcga atcgccgcga tgcagagcga ggccaccggg cgcaccgtcc tcgacgccgg
     4921 gcaacaggtt caccttatcg gtcgtcgcga ggagcgagcc gtcggggcca aacaggtgcg
     4981 acgagttcgt gatgctgccg tcggcgtgaa gccgcaggaa cgagcccgca acgacgtacg
     5041 cgttgtactg ccgcgcgagc ggaccaaaca gcgccttcca ccagcgttcg ccgtcgggcg
     5101 cgatcgcagc gagcacggcg tgacgcgcgt cgagcaatcg cgtgctcacg acgccgcgaa
     5161 gcacatcgag cggtcgccgc accgctgctt gtgcgagcgc cgacgcgagc gtcttcgctt
     5221 tgcgtgcgcg cggcggcgcg agtgcgtaga gcgcgaggtg accggcgact tccggaaaca
     5281 cgacgagccg cgcgtcgctg ccacccgaat cgagggcggt cgcggcggcg gcttcgaggt
     5341 gtcgccgata cgcgtccgca gatgcaatcg cttctgcgtc gacggcgagc tgaatcgcga
     5401 cgagctctac gttcacttcg gctgcgggac gacgcgcagg tacggcttca gcgtcttcca
     5461 gccgttcggg aactgcttct tcgcatcgtc gtccgacacc gacggaacga tgatgacgtc
     5521 gccgccctgc ttccagtcga ccggcgtcgc gaccttgtgc ttcgctgtga gctgcagcga
     5581 gtcgatgacg cgcaggatct cgtcgaagtt gcgaccggtg ctcgccgggt acgtgatcga
     5641 cagcttgatc ttcttgtcgg ggccgatgac gaacaccgag cgcaccgtca gcgtgtcgtt
     5701 cgcgttcgga tgaatcatgc cgtagaggtt cgcgacctcg cgcttcggat ccgcgatcag
     5761 cgggaagttg agcgcgtggc cctgcgtctc cttgatgtcg ccgacccagc gcttgtgatc
     5821 gtcgagcgga tcgaccgaga ggccgagcac tttgacgccg cgcttgtcga actcgggctt
     5881 gagcgccgaa acgcgaccga gctcggtcgt gcacaccggc gtgaagtctt tcgggtgcga
     5941 gaacagcacg ccccaaccac tgcccaagt
//
LOCUS       DATLBZ010000626         4526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8763179, whole genome shotgun sequence.
ACCESSION   DATLBZ010000626 DATLBZ010000000
VERSION     DATLBZ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8763179"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /gene="groL"
                     /locus_tag="VMZ53_30735"
     CDS             <1..1180
                     /gene="groL"
                     /locus_tag="VMZ53_30735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HUS32933.1"
                     /translation="EMIAEAMKKVGKEGVITVEEAKTLNSELDVVEGMQFDRGYLSPY
                     FVTDTERMEVVMNDVFILTHEKKISNMKELLPVLEQVAKQGKPLLIIAEDVDGEALAT
                     LVVNKLRGTLQVCAVKAPGFGDRRKEMLKDIAVLTGGQAVTEDLGLKLENLTLADLGQ
                     AKRITVDKDNTTIVDGSGKKPDIEARVKQIRNQVEETTSDYDREKLQERLAKLVGGVA
                     VIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVPGGGVALIRAQSALDGIKLSEEQ
                     AFGVSIVRRAIEEPLRQISSNAGVDGSIVVSKVKEGQGSFGFNAATLEYTDLVKAGVI
                     DPTKVVRTALQNAASVAALLLTTEAMIAERPKKEGAAPAGGGGGGGMGGMGGMGGMDF
                     "
     gene            complement(1230..1541)
                     /locus_tag="VMZ53_30740"
     CDS             complement(1230..1541)
                     /locus_tag="VMZ53_30740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32934.1"
                     /translation="MDEVPTDARTVTLGRRVRWLDRYRRTIAIALAVVALPLIMLELG
                     ELLGNDWPGVHATMLAAMLSFCSWWLIEVALAWMTAVWETEYDRRLRERGLPRARLLR
                     K"
     gene            complement(1585..1902)
                     /locus_tag="VMZ53_30745"
     CDS             complement(1585..1902)
                     /locus_tag="VMZ53_30745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32935.1"
                     /translation="MRHLLAAVLLATLAIGCKGDPEKCDKACRNYAELIFWEKANAEI
                     DAAPADKRDELRKTKLAEFTKNLELGINTCTSKCLSANNDDDTKCLLAAKTAQQAKDC
                     VTD"
     gene            1945..3075
                     /locus_tag="VMZ53_30750"
     CDS             1945..3075
                     /locus_tag="VMZ53_30750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32936.1"
                     /translation="MRRVALLLVCVASRAHAAPWTVTGEAGSEYDDNVQRVETGPGLD
                     TEHRSAWVLRFGARVEHKARAWGGQYSFDVSDLTRAVTDNELSVENVTALAGSLRWVH
                     ALPDRPVLVGVSTSAIDVFPLSDSTGARTFRNLGADALLAVRSDDHTLTLGFGGRDFV
                     YKAEASHIFDWRGPSASARLDLVLWQSPSKTKSLELASSAGFEDRTFVTEEAKAFANA
                     CAPDAPPDPGCAAPTSYGRRDRYSRVGTELTWVGHQVATIGYQLALIDSNSFGESLAR
                     HRVTASGTAAVGGGVYATLLAILQLDQYLDGLVIRRDLQGQGFANVDDENRSSLQLRV
                     AKKLYSEWSLETRVAVWRNVGGNSMQLDFQRELIYLGIVYGK"
     gene            3229..>4526
                     /locus_tag="VMZ53_30755"
     CDS             3229..>4526
                     /locus_tag="VMZ53_30755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32937.1"
                     /translation="MRHFLSAQGCLLLGLAFVGCSNLDLDPAPSIIHARFDPDASVIP
                     MPTDVLRDKAAGRLNLPNDTDKERAKLNAAETEFYQFLETLDGWSSLMSATVEFTGAI
                     DPKSISDGTVQVWHWGAVPERVSNARISLSADGKKLTIDPPRTGWLRGDRYVALVRGG
                     SKGLVGLANEKVECDAAFYFLRQTQQLDTPDHEHAFPGEDHGERVSNASRLEGIRTDL
                     VDAFDYFAAHDLPREDVAALWAFTVTTKTELAMDQPSQRIPLPIDLMIDPATGHVDAP
                     VAPWDSATEAEAKGRLSEMDGFALSGSQLFELTAPMSAASITEQTVQLYNIAGATPVL
                     EPATVELLADKTHLVVTPKSGRLPEKTRFAVVLRNGIKDAAGQTPVLMPIGHFLRSQS
                     PLLVDGHSQIRAIDDHDALKVETSRTELADALGKLGRDQVL"
BASE COUNT          803 a         1479 c         1497 g          747 t
ORIGIN      
        1 cgagatgatc gccgaggcga tgaagaaggt cggcaaggaa ggcgtcatca cggtcgaaga
       61 ggccaagacg ctgaacagcg agctcgacgt cgtcgagggt atgcagttcg accgcggcta
      121 cctctcgccg tacttcgtga cggacaccga gcgcatggaa gtcgtcatga acgacgtctt
      181 cattctcacg cacgagaaga agatctcgaa catgaaggag ctcctgccgg tcctcgagca
      241 ggtggcgaag caaggcaagc cgctcctcat catcgcggaa gacgtcgatg gcgaagcgct
      301 cgcgacgctc gtcgtcaaca agctccgtgg cacgctccaa gtctgcgccg tcaaggcgcc
      361 gggcttcggc gatcgccgca aggagatgct gaaggacatc gccgtcctca ccggcggcca
      421 agcggtcacc gaggatctcg gcctcaagct cgagaacctc acgctcgctg acctcggtca
      481 ggccaagcgc atcacggtcg acaaggacaa cacgacgatc gtcgacggct cgggcaagaa
      541 gcccgacatc gaggcgcgcg tgaagcagat ccgcaaccag gtcgaggaga ccacgtcgga
      601 ctacgatcgc gagaagctgc aagagcgcct cgcgaagctc gtcggcggcg ttgcggtcat
      661 caaggtcggc gctgcgaccg aagtcgagat gaaggagaag aaggctcgcg tcgaggacgc
      721 gctccacgcg acgcgcgctg ccgtcgaaga gggcgtcgtc cccggtggcg gtgtcgctct
      781 catccgcgcg cagtcggctc tcgacggcat caagctcagc gaagagcagg cgttcggtgt
      841 ttcgatcgtg cgtcgcgcga tcgaggagcc gcttcgccag atctcgtcga acgcgggcgt
      901 tgacggctcg atcgtcgtct cgaaggtcaa agagggtcag ggcagcttcg gcttcaacgc
      961 cgcgacgctc gagtacaccg acctcgtgaa ggccggcgtc atcgacccga cgaaggtcgt
     1021 ccgcacggca ctccagaacg cggcctcggt ggctgcgctc ctcctgacga ccgaggcgat
     1081 gatcgccgag cgtccgaaga aggaaggcgc tgcaccggcc ggtggcggcg gtggcggcgg
     1141 catgggtggt atgggcggca tgggcggcat ggacttctag tccacgctct ctcgctcgca
     1201 gatgcgcctc cgattcgtcg ggggcgtttc tactttcgga gcagccgtgc gcgaggcaag
     1261 ccgcgttcgc gcagtcgccg gtcgtactcg gtctcccaga cggcggtcat ccaggcgagc
     1321 gcgacctcga tcagccacca cgagcagaac gagagcatcg ccgcgagcat cgtcgcgtgg
     1381 acgccgggcc aatcgttgcc gagcagctcg ccgagctcga gcatgatgag cggcagcgct
     1441 acgacagcga gcgcgatcgc gatcgtacgg cggtagcggt cgagccagcg aacgcgacga
     1501 ccgagcgtga cggtgcgcgc gtcagtcgga acctcgtcca ttgccattag cgtagcagaa
     1561 ccgacgacgt cggtcggcga gccgttaatc ggtgacgcag tccttggctt gttgcgcggt
     1621 cttggcggcg agcaagcact tggtgtcgtc gtcgttgttc gcggacaggc acttgctcgt
     1681 gcacgtgttg atgccgagct cgaggttctt cgtgaattcg gcgagcttcg tcttccggag
     1741 ctcgtcgcgc ttgtctgccg gcgccgcgtc gatctcggcg ttggccttct cccagaagat
     1801 gagctcggcg tagttgcggc aggccttgtc gcacttttcc gggtcgccct tgcagccgat
     1861 ggcgagggtc gcgaggagaa cggcggctaa caggtgacgc atgcccctgc cctaccacgg
     1921 cagcggccgc gatacgatgc ggtcatgcgt cgcgttgccc tgctgctggt ttgcgtggca
     1981 tcgcgcgcac atgcagcccc gtggaccgtg accggcgaag caggcagcga gtacgacgac
     2041 aacgtgcagc gcgtcgagac cggcccgggc ctcgacaccg agcaccgcag cgcgtgggtc
     2101 ttgcgattcg gcgcgcgcgt cgagcacaag gcccgtgcat ggggcggcca atactcgttc
     2161 gacgtgagcg acctcacgcg cgcggtcacc gacaacgagc tgtccgtcga gaacgtgaca
     2221 gcgctcgccg gatcgctgcg ctgggtgcac gcgctgccgg accgtcccgt gctcgtcggc
     2281 gtgagcacca gtgcgatcga cgtgttcccg ctgtccgact cgacgggcgc gcgcacgttt
     2341 cgcaacctcg gcgccgatgc gctgctcgcc gtgcgcagcg acgatcacac gctcacgctc
     2401 ggcttcggcg gtcgcgactt cgtctacaaa gccgaggcct cgcacatctt cgactggcgt
     2461 ggaccgtcgg cgagcgcgcg tctcgatctc gtgctgtggc agtcgccgag caagacgaag
     2521 agcctcgagc tcgcgtcgtc ggcaggcttc gaggaccgca cgttcgtcac cgaagaagcg
     2581 aaggcgttcg cgaacgcgtg cgcgcccgat gcgccgccgg atccgggatg tgcggcaccg
     2641 acgagctacg gccgtcgcga ccggtactcg cgcgtcggga cggagctgac gtgggtcggg
     2701 caccaggtcg cgaccattgg ctatcagctc gcgctgatcg actcgaatag cttcggcgag
     2761 tcgctcgcgc gtcatcgcgt gacggcgtcg ggcaccgcgg cggtcggcgg cggcgtgtac
     2821 gcgacgctgc tcgcgatcct ccagctcgat caatatctcg atgggctcgt catccggcgc
     2881 gacctgcagg gccagggctt cgcgaacgtc gatgacgaga atcgcagctc gctccagctg
     2941 cgcgtcgcga agaagctcta ctccgagtgg tcgctcgaga cgcgcgtcgc ggtgtggcgc
     3001 aacgtcggcg gcaactcgat gcagctcgac ttccaacgcg agctgattta cctcggtatt
     3061 gtgtacggta agtaagcggt cttaccagtc taaccgcgcg cagctactgc acgatccagg
     3121 ttgcagcgcg aaattagcgt cagtcattga ccgcaagtca atggcgtctt gactcagttt
     3181 caataaacac cgacaaccgt gccgcgtcac tcaggagggg gaatctccgt gcggcacttc
     3241 ttatccgctc aaggctgtct gttactcgga ctcgcattcg tcggctgtag caacctggat
     3301 ctcgatccgg caccaagcat cattcacgcg cggttcgatc ccgacgcgtc ggtgattccg
     3361 atgccgaccg acgtgctccg cgacaaagcg gccggtcgcc tgaacctccc gaacgacacc
     3421 gacaaagagc gcgcgaagct caatgccgcc gagacggagt tctatcaatt cctcgagacg
     3481 ctcgacggtt ggtccagcct catgtcggcg acggtcgagt tcacgggcgc gatcgatccg
     3541 aagtcgatca gcgacggcac ggtgcaagtc tggcactggg gcgcggtgcc cgaacgcgtc
     3601 agcaacgcgc gcatctcgct gtcggccgac ggcaagaagc tgacgatcga tccgccgcgc
     3661 accggttggc tccgcggtga ccgctacgtg gcgctcgtgc gcggtggcag caaaggtctc
     3721 gtcggtctcg ccaacgagaa ggtcgagtgc gacgcggcgt tctatttcct gcgccagacg
     3781 cagcagctcg acacgcccga tcacgagcac gcattccccg gtgaggatca cggtgagcgc
     3841 gtgtcgaacg catcgcgcct cgaaggcatc cgcaccgatc tcgtcgatgc gttcgactac
     3901 ttcgccgcgc acgacctgcc gcgcgaagac gtcgctgcgc tgtgggcatt caccgtcacg
     3961 acgaagaccg agctcgcgat ggatcagccg tcgcagcgca tcccgctgcc gatcgacttg
     4021 atgatcgatc ccgcgaccgg tcacgtcgat gcgcccgtgg caccgtggga cagcgcgacc
     4081 gaggccgaag caaagggccg cctgtccgag atggacggct tcgcgctctc cggcagccag
     4141 ctattcgagc tcaccgcgcc gatgagtgcg gcgtcgatca ccgagcaaac cgtgcagctc
     4201 tacaacatcg ccggcgcgac gccggtcctc gagcctgcaa cggtcgagct gctcgccgac
     4261 aagacgcacc tcgtcgtcac gccgaagtcg gggcggctgc cggagaagac gcggttcgct
     4321 gtcgtccttc gcaatggcat caaggatgcg gccggccaga cgccggtgct gatgcctatc
     4381 ggccacttcc tccgctcgca atcgccgctg ctcgtcgacg gtcacagcca gattcgcgcg
     4441 atcgacgatc acgacgcgct caaggtcgag acctcgcgca ccgagctcgc agatgcgctc
     4501 ggcaagctcg gccgcgatca agtgct
//
LOCUS       DATLBZ010000627         6593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8763365, whole genome shotgun sequence.
ACCESSION   DATLBZ010000627 DATLBZ010000000
VERSION     DATLBZ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8763365"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VMZ53_30760"
     CDS             complement(<1..706)
                     /locus_tag="VMZ53_30760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32938.1"
                     /translation="METDTVQLTPGSSQELPLADDPVLLQMAINEIGLEMAGPSQSPW
                     DAPFDLRVARPRAIDLRKLWKLTGQAEPPGITASLGPRIPILLQHVITPFPVDGKAPG
                     KVWGLGYEFEPHDVDANTVSVAPDDEVLKIGQIGQEVEMGVELGGKIGVPQGALSALA
                     AAPAVSLTNASLRASTSQSFQFSLHLKLTLRKLVGAGFGAGGAQWKLYRQDEPIDKPH
                     TLLQTLLVAEGTKSIKC"
     gene            complement(718..2970)
                     /locus_tag="VMZ53_30765"
     CDS             complement(718..2970)
                     /locus_tag="VMZ53_30765"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HUS32939.1"
                     /translation="MSQRYGEDQLKQVAGRFSRARGRKPPATKSPLESLSPDDLGMGN
                     LVQAGGGEATDKPEVTPAVAKPQAAGAREVVDQILRDAQARSKGRFEDVRRSISNERI
                     IGRGDLQDLNYLEVGIAVARGVARIRVPGGSGTGSLIGPRIIMTNNHVIGSPAELDSR
                     SRAQFDVQDDADGRPLPQQAFALDPGFFFFTSKELDITLIGIAERSDAGRPASMYPWV
                     RIGPADSDLKDKDALTIIQHPLGGMKQIAFRNNDVIVVQDLPQFVHYTTDTQPGSSGS
                     PCFDDLWRLVALHHSGVPATNAEGKLLTRDKTVYDPAVHSADAILWVANEGARMSAIA
                     DVLKKAALSSDHAFRRDQFFGDEPPNPVALARQVPPPSPNEEQPSGPHRISSFKNGRL
                     SVVVDISGDEICGVHIERSPAAESSSFGVTQGPTGSLPAPSGPSQGPPSEPLNDTDET
                     ITIDKDWKARKGYDPDFLGIHIPLPMLNEEQRKVTLRVPDLYQKQGEDPLVFKYHHYS
                     IAFNKKRHIAWYSAANVDGDQRKHKYAREKDKWFFDERMDSKDQPVHQLGESFYAAAN
                     TDRGHLTRYLDVQWGATLDETRKATNDSFHFSNCALQVSGFNQGKDRWQGIERFLLEE
                     KARKEKRRMIVITGPVLRANDPFYRTEKMDAAVRIPLSFWKLCALVPNDDRDLAVTAF
                     VLGQPDAADLEDVEEKFDVTAVQVTVAKLAKITGFIFDKKLLDNDHFAKKGAGSLEAI
                     GLMRDEDIVV"
     gene            complement(3084..3773)
                     /locus_tag="VMZ53_30770"
     CDS             complement(3084..3773)
                     /locus_tag="VMZ53_30770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32940.1"
                     /translation="MAYRDNIDALAARKTALAAEVDTKQRELAQTSRMLEEIRAKARL
                     PVLPNIRVASPCTADWNAMTPVDADVERVRHCGSCDKNVYNLTQMTRDEAEALILAKE
                     GRLCVRYFQRKDGTILLKDCTVGVSKKRKRRLIAAGAAALLAGGAGAAATYIMHTDSK
                     GKTERYEAIAGGIGPTPGYELQGQLEPTPPPPELKAPKRVAKELEAKELPTAKKHVAK
                     KHAPPHAKRAK"
     gene            complement(3778..4470)
                     /locus_tag="VMZ53_30775"
     CDS             complement(3778..4470)
                     /locus_tag="VMZ53_30775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32941.1"
                     /translation="MTYRNDIDALSARHAALSAEVETKTRELDTTSRLLDEMKARAKL
                     PVLPNIRVASPCTADWKEMAPVDDVGERVRHCGSCDKNVYNLSQMTRDEAEALILAKE
                     GRLCVRYFQRKDGTILLKDCTVGASNSKKRRLIAAGAAAMLVGSGAAAAFMMRGDATP
                     AATIVQSSDEIPSMRGKIEVAHEDPPPPAPPSAHDNDWHEVKGDIDPGYEMKMGGISF
                     DPLEDNTLSKPD"
     gene            complement(4467..5045)
                     /locus_tag="VMZ53_30780"
     CDS             complement(4467..5045)
                     /locus_tag="VMZ53_30780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32942.1"
                     /translation="MRRPELGRLGLPVLPNIFVASPCPADWSKMTGDERVRMCGQCNK
                     HVFNLSQMNRSDAEELIVEKNGNLCVRYYQRKDGTILLKDCTVAASAARRRQIAAAAA
                     ATLLATGGGFLALTAHRSAASPQAARILAPAAPAEIDFRIREQEMAKDDAPPIVIPPP
                     LPPEELVLEHDIQVTGGAVAIDPSYFEAETNK"
     gene            complement(5097..>6593)
                     /locus_tag="VMZ53_30785"
     CDS             complement(5097..>6593)
                     /locus_tag="VMZ53_30785"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS32943.1"
                     /translation="LATLHAARLIHRDFTLRNILVDEGANVACLFDFDLAMSLDDVGN
                     QTFRNYYKGRIFGSPGWSVAPETIDQALMDSNINTSLDIYAIGGALHGLFTEQLLYGP
                     ADDMWALLIRIAEGVVVGGESKVHYPDNFPDALKPIVEGCLERDPAQRFPSVQTVISE
                     LRGVLRELPDETRRESKRRLQTSADEEKAKAISGVMAKQPGPDITEDQIRKAEFAVWE
                     WGYSVDKNLGKVRGHPVYLSNPRQDLISSGQFPDANTFPKLVTVIDLTKVQDPRSFVE
                     NWQRYFWPILKRVRQGLMTSLHKVIYDANSSSLLLFTEYVDEPRFGSRLADIDLNADG
                     ALALGFLVIRQVAALHEHGMAHNNISPGALLFKGVPATRTVVPAMIGLVEPAMGQEAM
                     AGDCRALAGMILEWLRPARIAALHVRIKPMFEALRTKLNAWAHDQATPAPSIDMLLAL
                     ISDALSLVDFNFSVLRDSGGDLQEYALLLISLRLYHALWPEPRANTAS"
BASE COUNT         1025 a         2016 c         2245 g         1307 t
ORIGIN      
        1 tgcacttgat cgacttcgtg ccttcggcga cgagcagcgt ctgcagcagt gtgtgcggct
       61 tgtcgatcgg ctcgtcctgg cggtacagct tccactgcgc gccgcctgcg ccgaagcccg
      121 cgccgacgag cttgcgcagc gtgagcttga ggtgcagcga gaattggaag ctctggctcg
      181 ttgatgcgcg cagtgacgcg ttggtcagcg agacggccgg cgcagccgcg agagcggaga
      241 gcgcgccttg ggggacgccg atcttgccgc cgagctcgac gcccatctcg acctcttggc
      301 cgatctgtcc gatcttgagg acttcgtcgt cgggcgcgac gctcacggtg tttgcatcga
      361 cgtcgtgcgg ctcgaattcg tagccgaggc cccagacctt gccgggcgcc ttgccgtcga
      421 ccgggaatgg cgtgatcacg tgttggagca aaatcggaat tcgcgggccg agcgatgcgg
      481 tgatgcctgg cggctcagcc tgtcccgtga gcttccacag cttgcggaga tcgatggcgc
      541 gaggccgcgc gacgcgaaga tcgaacggcg cgtcccacgg cgactgagaa gggcctgcca
      601 tctcgaggcc gatctcgttg atcgccatct ggagcagcac gggatcatcg gcgagcggta
      661 gctcttgcga cgagcctggc gtgagttgaa cggtgtcggt ttccatcgtc atttccttta
      721 gaccacgatg tcttcgtcgc gcatgaggcc gatcgcttcg agcgagccag cgcccttctt
      781 cgcgaagtga tcgttatcga gcagcttctt gtcgaagatg aagccggtga tcttggcgag
      841 cttcgcgacc gtgacttgca ccgctgtgac gtcgaacttc tcctcgacgt cctcgagatc
      901 tgcggcgtcc ggttgtccga ggacgaacgc ggtgaccgcg aggtctcggt cgtcgttggg
      961 cacgagcgcg cacaacttcc agaacgagag cgggatgcgc acggccgcgt ccatcttctc
     1021 ggtgcggtag aacggatcgt tcgcacgcag aaccggcccg gtgatcacga tcatccggcg
     1081 cttttccttg cgcgcttttt cctcgagcag gaagcgctcg atgccttgcc agcgatcttt
     1141 gccttggttg aagccggaga cttggagcgc gcagttcgag aagtggaacg agtcgttcgt
     1201 tgctttgcga gtctcgtcga gggtggcgcc ccactgaacg tcgaggtagc gcgtcaggtg
     1261 gccgcggtcg gtgttcgctg cggcgtagaa cgattcgccg agctggtgga ccggttggtc
     1321 tttgctgtcc atgcgctcgt cgaagaacca cttgtccttt tcgcgagcgt acttgtgctt
     1381 gcgctgatca ccgtcgacgt ttgcggcgga gtaccaggcg atgtgccgct tcttgttaaa
     1441 cgcgatcgag tagtggtggt acttgaagac gagcgggtct tcaccctgct tctgatagag
     1501 gtcagggacg cgcagcgtta ctttgcgctg ctcttcgttg agcatcggaa gcgggatgtg
     1561 gatcccgagg aagtcggggt cgtagccttt gcgagctttc cagtctttgt cgatcgtgat
     1621 cgtctcgtcg gtgtcgttca gtggctcgga tggcggacct tgcgacggac ccgacggcgc
     1681 tggcagcgag ccagtcggac cttgcgtcac gccgaagctc gagctctcgg cggcggggct
     1741 gcgttcgatg tgaacgccgc agatctcgtc gcctgaaata tcgacgacga cggacagccg
     1801 cccgttcttg aacgagctga tccggtgcgg gccgctcggc tgctcctcgt tcggcgaggg
     1861 aggaggcacc tgcctcgcga gtgcgacggg attgggcggc tcgtcgccga agaactggtc
     1921 gcggcgaaat gcgtggtcac tcgacagcgc tgccttcttg aggacgtccg cgatcgccga
     1981 cattcgcgcg ccctcgttcg cgacccacag gatcgcatcg gcggagtgca ccgccgggtc
     2041 gtagacggtc ttgtcacgcg tgagcagctt gccttccgcg ttggtcgcgg gaacgcccga
     2101 gtgatggagt gcgacgagcc gccagagatc gtcgaagcac ggcgagccac tcgaaccggg
     2161 ctgcgtatcc gtcgtgtagt ggacgaactg cggcaggtct tgcaccacga tcacgtcgtt
     2221 gttgcggaac gcgatctgct tcatgccgcc gagcggatgc tggatgatcg tgagcgcgtc
     2281 tttgtctttg agatcggaat cggcgggccc gatccgcacc cacggataca tcgaggcggg
     2341 tcggccggcg tcggagcgct cggcgattcc gatcagcgtg atgtcgagct ccttcgacgt
     2401 gaagaagaaa aatcccggat cgagcgcgaa tgcctgctgt ggcagcgggc gcccatcggc
     2461 gtcgtcctgc acgtcgaact gcgcgcgcga gcgactatcg agctcggccg gtgaaccgat
     2521 gacgtggttg ttcgtcatga tgattcgcgg accgatcagc gagccggttc ccgagccacc
     2581 cggcacgcga atgcgggcga cgccgcgagc taccgcgatg ccgacctcga ggtaattcag
     2641 atcttggagg tcgccgcgac cgatgatgcg ctcgttcgag atcgagcgac ggacgtcctc
     2701 gaagcgacct ttgctgcgcg cttgcgcatc gcgcaggatc tgatcaacga cctcacgcgc
     2761 gccggccgct tgcggtttcg cgacggcagg cgtcacttcc ggcttgtcgg ttgcctcgcc
     2821 gccgcctgct tgtacgaggt ttcccatgcc caggtcgtcc gggctcagcg actcgagcgg
     2881 gctcttcgtc gccggtggct tgcgaccacg tgcgcgactg aaccgacccg caacttgctt
     2941 tagctgatcc tcgccatacc tttggctcac ggtcccctcc ttctgagagc gatccccata
     3001 tcgctcagag gggacgatga gcccatattt caatcgcctg cacaagcgcg ggccgtgcag
     3061 cgaattgttg cggcgaccgc gagctacttc gcgcgcttcg cgtgcggcgg cgcgtgcttc
     3121 ttcgcgacgt gcttcttcgc ggtgggcagc tccttcgcct cgagctcctt ggcgacgcgc
     3181 ttcggcgctt tgagctctgg cggcggcggc gtcggctcga gctgaccctg tagctcgtag
     3241 cctggcgtcg gtccgattcc accggcgatc gcctcgtagc gctcggtctt gcccttgctg
     3301 tcggtgtgca tgatgtacgt cgccgctgcg cccgcaccac ctgcgagcag cgccgcggca
     3361 cctgccgcga tcaggcggcg cttgcgcttc ttcgagacgc cgaccgtgca atctttgagc
     3421 aagatcgtgc cgtctttgcg ctggaagtag cgcacgcaga ggcgaccttc cttcgcgagg
     3481 atgagcgcct cggcttcgtc gcgcgtcatc tgcgtcaggt tgtagacatt cttgtcgcag
     3541 ctgccgcagt ggcggacgcg ttcgacgtcg gcgtcgacgg gcgtcatcgc attccagtcc
     3601 gcggtgcacg gcgacgcgac gcggatgttc ggcaggactg gcaaccgcgc ctttgctcgg
     3661 atctcttcga gcatgcgact tgtctgcgcg agctcgcgct gtttggtgtc gacctcggcg
     3721 gcgagtgcgg ttttgcgcgc tgcgagcgcg tcgatgttgt cgcggtacgc catcgcgtca
     3781 gtccggcttc gagagcgtgt tgtcttcgag cgggtcgaag gagatgccgc ccatcttcat
     3841 ctcgtaaccg ggatcgatgt cgcctttgac ctcgtgccag tcgttgtcgt gcgcgctagg
     3901 cggcgcgggc ggcggcggat cttcgtgtgc gacctcgatc ttgcctcgca tcgacggaat
     3961 ctcgtcggag ctctggacga tcgtcgccgc gggcgttgcg tcgccgcgca tcatgaatgc
     4021 ggcagctgcg ccactaccga cgagcatcgc ggcagcaccg gctgcgatga ggcggcgctt
     4081 cttactgttc gatgcgccga ccgtgcagtc tttcaataga atcgtgccgt ccttgcgctg
     4141 gaagtaccgc acgcagaggc ggccttcctt cgcgaggatg agcgcttcgg cttcgtcgcg
     4201 cgtcatctgc gagaggttgt agacattctt gtcgcagctg ccgcagtgac ggacgcgttc
     4261 gccgacatcg tcgacgggcg ccatctcttt ccagtcggcc gtgcatggcg acgcgacgcg
     4321 gatgttcggc aggaccggca gcttcgcgcg cgccttcatc tcgtcgagca ggcggctcgt
     4381 cgtgtcgagc tcgcgcgtct tcgtctcgac ttccgcggag agagcggcat ggcgagcgga
     4441 gagcgcgtcg atgtcgttgc gatacgtcac ttgttggtct ccgcttcgaa gtaggaggga
     4501 tcgatcgcga ccgcgccgcc ggtgacttga atgtcgtgct cgagcacgag ctcctcgggc
     4561 ggaagcggtg gaggtatgac gatcggcggc gcgtcgtcct tggccatttc ctgctcgcgg
     4621 attcgaaaat cgatctcggc gggagccgcc ggcgcgagga tgcgagccgc ttgcggcgag
     4681 gcggcggagc gatgcgccgt cagcgcgagg aagccaccgc ccgttgcgag gagagtggca
     4741 gctgcggccg cggcgatctg gcggcgacgt gcggccgatg ctgcgacggt gcagtccttg
     4801 agcaggatcg tcccgtcttt gcgctggtag tagcgaacgc acaggttgcc gttcttctcg
     4861 acgatgagct cttccgcatc gctgcggttc atctgagaca ggttgaacac gtgcttgttg
     4921 cactgaccgc acatccggac gcgttcgtcg ccggtcatct tcgaccagtc ggccgggcac
     4981 ggcgacgcga cgaaaatgtt tgggagtacg gggagaccca acctgccgag ctcgggacgg
     5041 cgcatgggta tttcaaacgc gcggcggaag cggccggtct acgggagtag gcgacgctaa
     5101 ctggctgtat tagctcgcgg ttccggccac agcgcgtggt acagccgaag cgaaatcagg
     5161 agcagcgcgt actcctgcag gtcgccaccc gagtcgcgca gcaccgagaa gttgaagtcg
     5221 acgagcgaca gcgcgtcgct gatcagagcg agcagcatgt cgatcgaggg cgctggcgtc
     5281 gcttgatcgt gtgcccacgc gttgagcttc gtgcgcagcg cctcgaacat cggcttgatg
     5341 cggacgtgaa gcgcggcgat gcgtgcgggg cgaagccact cgaggatcat gccggcgagc
     5401 gcgcggcagt ctcccgccat cgcctcctgg cccatcgcgg gctcgacgag accaatcatc
     5461 gcagggacga cggtgcgagt ggcgggtacg cctttgaaga gcagcgcgcc cggcgagatg
     5521 ttgttgtggg ccatgccgtg ctcgtgcagc gcggcgacct gacggatgac gaggaagccg
     5581 agcgcgagcg cgccgtcggc gttgagatcg atatcggcga ggcgagagcc gaagcgaggc
     5641 tcgtcgacgt actcggtgaa cagcagcagc gagctcgagt tcgcgtcgta gatgactttg
     5701 tgcagcgagg tcatcaggcc ttgccgcacg cgcttcaaga tcggccagaa gtaccgctgc
     5761 caattctcga cgaacgagcg cgggtcctgc accttcgtca gatcgatgac ggtgacgagc
     5821 ttcgggaacg tgttggcatc ggggaactgt cccgacgaga tgaggtcctg gcgcgggttt
     5881 gacagataga cgggatggcc gcgcaccttg ccgagattct tgtcgacgga gtagccccac
     5941 tcccacaccg cgaactccgc cttgcggatt tgatcctcgg tgatgtcggg accgggctgc
     6001 tttgccatca cccccgagat cgccttcgcc ttttcctcgt cggcgctcgt ctgcaggcgg
     6061 cgcttcgatt ctcggcgcgt ctcgtcgggc agctcgcgca ggacgccgcg cagctcgctg
     6121 atcaccgtct gcaccgacgg aaaccgctgc gctggatcgc gctcgaggca accttcgacg
     6181 atcggcttga gcgcgtcggg gaagttgtcc ggatagtgga ccttgctctc gccaccgacg
     6241 acgacaccct cggcgatccg gatcaagagc gcccacatat cgtcggcggg cccgtagagc
     6301 agctgctcgg tgaacaggcc gtggagcgcg ccgccgatcg cgtagatgtc gagcgacgtg
     6361 ttgatgttcg agtccatcaa cgcctgatcg atcgtctcgg gcgcgacgga ccaaccgggc
     6421 gagccgaaga tgcggccctt gtagtaattg cgaaacgtct ggttgccgac gtcgtcgagc
     6481 gacatcgcga gatcgaagtc gaacaggcac gcgacgttcg cgccttcgtc gacgaggatg
     6541 ttgcgcagcg tgaagtcgcg atggatgaga cgtgcggcgt gcagcgtcgc gag
//
LOCUS       DATLBZ010000628         7073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3562469, whole genome shotgun sequence.
ACCESSION   DATLBZ010000628 DATLBZ010000000
VERSION     DATLBZ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3562469"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VMZ53_30790"
     CDS             <1..346
                     /locus_tag="VMZ53_30790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32944.1"
                     /translation="AVLAVTRADVPNSDAWSKEKAKREAYATQVFAGVKAAMTGSKKV
                     TQKLGEMNNVPALDCEGTKDGAPVLVRVLMFRTYALSLAVELPKGADAKALKDARDIL
                     AKFGPTKPVPKD"
     gene            complement(343..1578)
                     /locus_tag="VMZ53_30795"
     CDS             complement(343..1578)
                     /locus_tag="VMZ53_30795"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HUS32945.1"
                     /translation="MSEVELEVTALAAGGDGVARDEQGRVTFIPRTAVGDVVRVQFVE
                     EKKRFARGEVFGIVKPSRDRVEPPCPFFKMGCGGCQWQHISREGQLVAKQTIVEGALR
                     KLPGLVVEKIADPSPPYGWRRRARFHVVNGQVGLFAYATNQLLPIDRCPQLEPQLDDA
                     LRAAIAEEPVDGELHLLLGHDGTVAVTVGDAAVDVGRAAAETIARAAGDIATAAADQV
                     IEAARAASKAAATGLAASEVIEIEPGLQGRAVDFAQASRAGNSALISITRAALGKGPG
                     KLLELYAGAGNLTRAFLEDGWSIVASDVVAPAKVREGVRFELGNARDVTQRLTKRKNT
                     AFDAVVLDPPRTGAAEAIESIAQLEPKTIVYVSCDPATLARDAQTLVDAGYRAERAWP
                     IDMMPQTAHVEVVMKLTRA"
     gene            complement(1622..2407)
                     /locus_tag="VMZ53_30800"
     CDS             complement(1622..2407)
                     /locus_tag="VMZ53_30800"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HUS32946.1"
                     /translation="MPIITLTTDFGARDGYVGAVKGVITRVVREVPAARHADRHLDII
                     DIAHDIPRGDIAHAAWVVATSTREFPPDTIHVVVVDPGVGTTRQAVIVDAEGQRYVGP
                     DNGVFGYLENPTAHAIAKTALLAPKISTTFHGRDIFAHVAARLASGYKPPDFGKATRL
                     TGTLPWGKRPEGLGRIVHIDIYGNLISDMPAREAGGHVLFGARRIPVGRTYEDVPAGE
                     VVAYVGSAGTIEIAVREGRADNAFAATRGMKVLPDPEGTGPYR"
     gene            2434..3138
                     /locus_tag="VMZ53_30805"
     CDS             2434..3138
                     /locus_tag="VMZ53_30805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HUS32947.1"
                     /translation="MNKVYPDAKAALHDVFDGCTLLSGGFGLSGNPENCIQELARRKV
                     MNLTIVSNNCGTTDKGLGVLLAQGQVRKMVSSYVGENKIFEQLFLSGKLEVELNPQGT
                     LAERLRAGGAGVEAFFTPTGYGTKVAEGKETREINGRLCVLEHAIRGDFAIVKAWKGD
                     KWGNLVFNKTSRNFNPLCAQAGKVTIAEVEELVEVGELDPDQIHLPSIYVHRIFQGTN
                     YEKPIEQRTVRKRSAS"
     gene            3135..3794
                     /locus_tag="VMZ53_30810"
     CDS             3135..3794
                     /locus_tag="VMZ53_30810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HUS32948.1"
                     /translation="MNRLDRDQMARRVAAELQDGFYVNLGIGMPTLVANFVPTGMDII
                     LQSENGMLGIGPFPYEGEEDPDLINAGKQTVTTIKGSAFFDSAQSFAMIRGGKVDVSV
                     LGALEVSMRGDLANWMIPGKMIKGMGGAMDLVAGSRRVIVMMEHVAKDGSPKILKDCT
                     LPLTGVQCVHTIVTDLAVIDVTPKGLVLRERAPGNTTDDIVKATGAPLIVSGDVPEMR
                     V"
     gene            3810..6065
                     /locus_tag="VMZ53_30815"
     CDS             3810..6065
                     /locus_tag="VMZ53_30815"
                     /inference="COORDINATES: protein motif:HMM:NF025223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32949.1"
                     /translation="MKWWFAALCAVCAVACSKTDSVLCGDGTVCPTGTVCLDADRAPN
                     RCVLDEQTTVCDGLRDGAFCEGPAFTGICDQGVCLPGCGDGNQDDGEQCDDGNFASHD
                     GCSSRCLSEAPTWAQYHGPWAARTQHSAAYDKTTGKLVIFGGVTPHGQDGATWIFDAQ
                     GWHRLEGNGPPGLAGASMVFDANRNVFVLFGGNGIDGAGTNETWELSLAGQWQKAAPS
                     ASPPRRTLAAMAYDSFRKKTVLFGGTTDAKTIVELDDTWEYNGTTWTKLTTATIPESR
                     YGAAITYDPVRREVVMFGGRRDVGRRADTWRLSSTTLDWAKFPVTGTTVVPLGRERSA
                     MAFDDIRNTCVLFGGQIAGQNPTNDLWEWNGAGWSQLATSVAPPPRDEHVLAFVVDPT
                     STPLTSKLMVIGGEATQLYDDVWKLEAAADPMRWRTVDLGLMPESRTAAQTYIPPTGE
                     IVMFGGTPVSLLNPADFWAFGPRGWRRIAAGLTARGVNGLAYDAAGTRLIAVGGANAN
                     GTPVQTTDVWNGTAWLTIAPSPVLPELRNPAFAFDEAGLGLLFGGESDRNNVLETFGD
                     TWELTGTTWTKTVSASDPSTPPPAASAALIYDPSRSAFLLVAADGKTWSYANHAWSQL
                     PTFKSPPPREGEQLIYDSLRQRIVMLGGYDSNTYYTNDLWQLDTLPNGIAIWHLLHVV
                     GEPPPPRRQTRAAYSPRLRAIMMFGGSDPTRLGDTWLLRFSSSTPDEFCFDTVDNDND
                     AQIDDVDPDCQ"
     gene            complement(6097..>7073)
                     /locus_tag="VMZ53_30820"
     CDS             complement(6097..>7073)
                     /locus_tag="VMZ53_30820"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS32950.1"
                     /translation="APLGTQPMGTVPVGSRSDMTPVPQGGAFQTPGSGTGPAMAHPGG
                     GPIVSTKHGYASAAMSRGDVSYGNYTMPVDAYGDVQLKPNRRPLFIGLGLAIVGIIVI
                     LAVSFGGKEEKAASPQPDEGSDGDTTETTMPQRGSEALPQPIEENMVSIVIDSEPHGA
                     DVLIAGTKIGTTPFNKKLKRGTSRAELKVKLAGYADFATKIDLAGDYENPSIKLTKVE
                     DAAAKGSADTTNSSTDAGASTTDKTGTGSTTGTGATTGTGATTSSTGTKTGTTGTGTG
                     TGTKTGTSATGTGTGTGTKTGTPTTKPKCQPPGPNVDPFSGVPVCPAK"
BASE COUNT         1207 a         2343 c         2327 g         1196 t
ORIGIN      
        1 agcggtcctt gcggtcacgc gtgcagacgt tccgaattcc gacgcgtggt cgaaggagaa
       61 ggcaaagcgc gaggcatacg ccacgcaggt tttcgcgggc gtgaaggccg ccatgaccgg
      121 ctcgaagaag gtcacacaga agctcggcga gatgaacaac gtgcctgcgc tcgactgtga
      181 agggacgaag gatggcgcgc cggtcttggt gcgcgtgctg atgttccgca cgtacgcgct
      241 gtcactggcg gtcgagctcc cgaagggcgc cgatgcaaag gcactcaaag acgcgcgaga
      301 catcctcgcg aagttcggac cgacgaagcc cgtgccgaag gattaggcgc gggtcagctt
      361 catcacgact tcgacgtgcg ctgtctgcgg catcatgtcg atcggccacg cgcgctccgc
      421 acgatagccg gcatcgacga gcgtctgtgc atcacgcgcg agcgtcgccg ggtcgcacga
      481 gacatagacg atcgttttcg gctcgagctg cgcgatggac tcgatcgcct cggctgcgcc
      541 cgtgcgcggt ggatcgagga cgacggcatc gaacgcggtg ttcttgcgct tggtcaggcg
      601 ctgcgtgaca tcacgtgcgt tgccgagctc gaaccgcacg ccttcgcgca ccttcgccgg
      661 cgcaacgaca tccgatgcga cgatcgacca gccatcttcg aggaacgcac gcgtcagatt
      721 gcccgcgccg gcgtagagct cgaggagctt gcctggacct ttgccgaggg cagcgcgcgt
      781 gatcgagatg agcgctgaat tgccagcgcg gctcgcctgc gcgaaatcga cagcgcgtcc
      841 ttggagcccg ggctcgatct cgatgacctc gctcgcggcg agccccgtcg cagccgcctt
      901 gctcgcggcg cgtgctgcct cgatgacctg atcggcggcg gccgttgcga tgtcgcccgc
      961 ggcgcgcgcg atcgtctctg cggctgcacg accgacgtcg accgccgcat cacccacagt
     1021 gaccgcaacc gtgccgtcgt gaccgagcag caggtggagc tcgccgtcca ccggttcttc
     1081 ggcgatcgcc gcgcgaagtg catcgtcgag ctgcggctcg agctgcgggc agcgatcgat
     1141 cggaagcaac tggttcgtcg cgtatgcgaa caggccgacc tggccgttga ccacatggaa
     1201 gcgagcgcgt cgtcgccagc cgtacggagg cgatggatcc gcgattttct cgacgacgag
     1261 gcccggcagc ttgcgcagcg cgccttcgac gattgtctgc tttgcgacga gctggccctc
     1321 gcgcgagatg tgctgccact gacaaccgcc acagcccatc ttgaaaaacg ggcacggcgg
     1381 ctccacgcga tcgcgcgatg gcttgacgat cccgaatact tctccgcgcg cgaaccgctt
     1441 cttctcttcg acgaactgta cgcgtacgac gtcacccacc gccgtgcgcg ggatgaacgt
     1501 caccctgccc tgctcatcgc gcgcgacgcc gtcgccaccc gcggcgagcg ctgtgacttc
     1561 tagctcgacc tcggacatcg cgcgcatcct ttcactacgg cgagcgcgag tccatgaggg
     1621 ctcagcgata agggcccgtg ccctcaggat ccgggagcac cttcatgccc cgggtcgctg
     1681 cgaacgcatt gtccgcgcgt ccctcgcgca ccgcgatttc gatcgtgcca gcggagccaa
     1741 cgtatgcgac cacctcgcca gcgggaacat cttcgtaggt gcggccgacg ggaatgcgcc
     1801 gcgcgccgaa gagcacgtgg cctcccgctt cgcgcgcggg catatcgctg atcaagttgc
     1861 cgtagatgtc gatgtggacg atgcggccga ggccttcagg gcgcttgccc cagggcagcg
     1921 tgccggtaag ccgcgtcgcc ttgccgaaat cgggcggctt gtagccactc gcgaggcgag
     1981 ctgcgacgtg cgcgaagatg tcgcggccgt ggaacgtcgt gctgatcttc ggagcgagca
     2041 gcgccgtctt cgcgatcgcg tgcgcggttg gattctcgag gtagccgaac acgccgttgt
     2101 cggggccgac gtagcgctgg ccctcggcgt cgacgatgac agcttggcgc gtggtcccca
     2161 cgccggggtc gacgacgacg acgtgaatcg tgtccggcgg gaattcgcgc gtcgaggtcg
     2221 cgaccaccca cgccgcatgc gcgatgtctc cgcgcgggat gtcgtgcgcg atgtcgatga
     2281 tgtcgaggtg gcgatcggcg tggcgtgcag cgggcacctc acgcacgact ctcgtgatga
     2341 cgcccttcac agcgccgacg taaccgtcgc gcgcgccgaa atccgtcgtc aacgtgatga
     2401 tcggcacccg gatcagaagt aagacacagg tcgatgaaca aggtctaccc ggacgccaag
     2461 gcggcgctcc acgacgtgtt cgacgggtgc accctcctat cgggtgggtt cggactttcc
     2521 ggcaaccccg agaactgcat ccaggagctt gcgcgacgca aggtgatgaa cctcaccatc
     2581 gtctcgaaca actgcggcac gacggacaag ggcctcggtg tcctgctcgc ccaaggccaa
     2641 gtgcgcaaaa tggtctcgag ctacgtcggc gagaacaaga tcttcgagca gctgttcctc
     2701 agcggcaagc tcgaggtcga gctcaacccg caaggcacgc tcgccgagag actgcgtgcg
     2761 ggcggcgctg gcgtcgaggc gttcttcacg ccgaccggct atggcacgaa ggtcgccgaa
     2821 ggcaaagaga cgcgagagat caacggccgc ctgtgcgtgc tcgagcatgc gattcgcggc
     2881 gacttcgcga tcgtgaaggc atggaaaggc gacaagtggg gcaacctcgt cttcaacaag
     2941 acgagccgca acttcaaccc gctgtgcgcg caggccggca aggtcacgat cgccgaggtc
     3001 gaagagctcg tcgaagtcgg tgagctcgat cccgatcaga ttcacctgcc ctcgatctac
     3061 gtgcaccgga tcttccaggg cacgaactac gaaaagccga tcgagcagcg caccgtgcga
     3121 aaaaggagcg cgtcatgaac cgtctcgatc gcgatcaaat ggcgcgtcgc gtagctgccg
     3181 agctgcaaga cggcttctac gtgaacctgg gcatcggcat gccgacgctc gtggcgaact
     3241 tcgtccccac cggcatggac atcattctcc agtccgagaa cggcatgctc ggcatcggtc
     3301 cgttcccgta cgaaggcgaa gaagatccgg atctgatcaa cgccggtaag caaacggtga
     3361 cgacgatcaa gggcagcgca ttcttcgact cggcgcagtc gttcgcgatg atccgcggcg
     3421 gcaaggtcga cgtcagcgtg ctcggcgcgc tggaagtcag catgcgaggc gacctcgcga
     3481 actggatgat cccaggcaag atgatcaaag gcatgggcgg cgcgatggat ctcgtcgccg
     3541 gctcgcgtcg cgtgatcgtc atgatggagc acgtcgcgaa ggacggctcg ccgaagatcc
     3601 tcaaggactg cacgttgccg ctgacgggcg tgcagtgcgt ccatacgatc gtgaccgacc
     3661 tcgccgtgat cgacgtgacg ccgaagggtc tcgttctgcg agaacgcgcg ccggggaata
     3721 cgaccgacga catcgtcaaa gcgacgggtg caccgctgat cgtgagcggc gacgtccccg
     3781 agatgcgcgt ctaggttact ctcgaccgca tgaaatggtg gttcgctgcg ctgtgcgcag
     3841 tgtgtgcggt tgcgtgctcc aagaccgatt cggtcttgtg tggagacgga acggtatgcc
     3901 cgacgggaac ggtctgcctc gacgccgacc gggcgccgaa tcgctgcgtg ctcgacgagc
     3961 aaacaacggt gtgcgacggc ctacgcgacg gcgcgttctg cgagggcccg gctttcaccg
     4021 gcatctgcga tcagggcgtg tgtcttcccg gttgcgggga cggcaaccaa gacgacggcg
     4081 agcaatgcga cgacggcaac ttcgcgagtc acgacggctg ctcgtcgcgc tgccttagtg
     4141 aggcgcctac gtgggcgcag tatcacgggc cgtgggccgc gcgcacgcag cactcggctg
     4201 cgtatgacaa gacgacgggc aagctcgtca tcttcggtgg cgtgacgccc cacggccagg
     4261 acggcgcgac atggatcttc gacgcgcaag gttggcaccg gctcgaggga aatggcccgc
     4321 cagggctcgc gggcgcgtcc atggtgttcg atgcgaaccg caacgtgttc gtcctgttcg
     4381 gcggaaatgg catcgacggc gccggcacga acgagacatg ggagctgtcg cttgccggcc
     4441 aatggcagaa ggcagcaccg agtgcgtcgc cgccgcgccg cacgcttgcc gcgatggcgt
     4501 acgactcgtt tcgcaagaag accgtgctgt ttggtggcac gaccgacgcg aagacgatcg
     4561 tcgagctcga cgacacgtgg gaatacaacg gcacgacgtg gacaaagctg accacggcga
     4621 cgattccgga gagccgctac ggcgcagcca tcacctacga tccagtgcgt cgcgaagtcg
     4681 tcatgttcgg tggccgcaga gacgtcgggc ggcgcgccga tacgtggagg ctctcgagca
     4741 cgacgctcga ctgggcaaaa tttccggtca cggggacgac cgtcgtgcca ctcgggcgcg
     4801 agcgaagcgc gatggcgttc gacgacattc ggaacacgtg tgtgctgttc ggtggccaga
     4861 tcgccggcca gaatccgacc aacgacctgt gggaatggaa cggcgcgggc tggtcacagc
     4921 tcgccacgag cgtggcgccg ccgccccgcg acgagcatgt cctcgcgttc gtcgtcgacc
     4981 cgacgtccac accgctgacg tcgaagctga tggtgatcgg cggcgaggcg acccagctct
     5041 acgacgacgt gtggaagctc gaagctgctg ccgacccgat gcgttggcga acggtcgatc
     5101 tcggcctcat gcccgaatcg cgcacggctg cgcagacgta cataccgccg acgggcgaga
     5161 tcgtgatgtt cggcggtaca ccggtgtcgc tgctcaatcc cgccgacttc tgggcgttcg
     5221 gtcctcgcgg ttggcgtcgc atcgcggctg gcctcaccgc gcgtggcgtc aacgggctcg
     5281 cctacgacgc cgctggcacc cgactcatcg ccgtcggcgg cgcgaacgcg aacggcactc
     5341 cagtccaaac gacagacgta tggaacggca cggcgtggct gaccatcgcg ccctcgccgg
     5401 tcctcccgga gctgcgcaat ccagcgttcg ctttcgacga agccggcctc ggcctgctgt
     5461 tcggcggcga atccgatcgc aacaacgtcc tcgaaacctt cggcgacacg tgggagctca
     5521 ccggcacgac gtggacgaag acggtctcgg cgagcgatcc gagcaccccg ccccccgccg
     5581 catccgctgc cctgatttac gacccgtcgc gcagcgcgtt cctcctcgtc gccgccgatg
     5641 gcaagacgtg gtcgtacgcg aaccacgcat ggtcgcaact gccgacgttc aagtcgccgc
     5701 caccgcgcga aggcgaacag ctgatctacg actccctccg ccaacgcatc gtgatgctcg
     5761 gcggatacga ctccaacacg tactacacga acgacctctg gcagctcgac acacttccca
     5821 acggcatcgc gatctggcac ctgctccacg tcgtcggcga gccgccgcca ccccgtcgcc
     5881 aaactcgcgc cgcatactcg ccgcggctcc gcgccatcat gatgttcggc ggcagcgatc
     5941 cgacgcgcct cggcgacacg tggctgcttc gcttctcgtc gagcaccccc gacgaattct
     6001 gctttgacac tgtcgacaac gacaacgacg cgcagatcga cgacgtcgac ccggactgcc
     6061 agtaggcgcg cttacgcgct gtccgaagcg cggacgctac ttcgccgggc agaccggaac
     6121 gccgctgaac ggatcgacgt tggggcccgg cggctggcac ttcggcttcg tcgtgggggt
     6181 gccggtcttc gtgcccgtgc cggtgcctgt gcctgtcgct gacgtgccgg tcttcgtgcc
     6241 cgtgccggtg cctgtgcctg tcgtgccggt cttcgtgccg gtcgacgatg tcgtcgcgcc
     6301 cgtacccgtc gtcgcgccgg tgcccgtcgt cgatcccgtg cccgtcttgt cggtcgtcga
     6361 tgcgccggcg tcagtcgacg agttggtcgt gtccgcgctg cccttcgcgg cggcatcttc
     6421 gaccttcgtg agcttgatgc tcgggttctc gtagtcgcct gcgagatcga tcttcgtcgc
     6481 gaagtcggcg tagcccgcga gctttacctt gagctcggct cggctcgtgc cgcgcttcag
     6541 cttcttgttg aacggcgtcg tgccgatctt cgtacccgcg atcaacacgt ctgccccgtg
     6601 cggctcggag tcgatcacga tcgagaccat gttctcttcg atcggctgcg gcagcgcctc
     6661 cgagcccctt tgcggcatcg tcgtttcggt cgtgtcgccg tcggagcctt cgtccggctg
     6721 tggcgaagcc gccttctctt ccttgccgcc gaacgagacc gcgaggatca cgatgatgcc
     6781 gacgatcgcg aggccgaggc cgatgaacag cgggcggcga ttcggcttga gctgcacgtc
     6841 gccgtacgcg tcgaccggca tcgtgtaatt gccgtacgag acgtcgccgc ggctcatcgc
     6901 cgccgatgcg tagccgtgct tcgtgctgac gatcggtcca ccgcccgggt gcgccatcgc
     6961 aggtccggta cccgagcccg gcgtctggaa tgcaccgccc tgcggcaccg gcgtcatgtc
     7021 cgaacgcgag ccaaccggaa cggtgcccat cggctgcgtg ccgagcggcg ctt
//
LOCUS       DATLBZ010000629        12441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3563588, whole genome shotgun sequence.
ACCESSION   DATLBZ010000629 DATLBZ010000000
VERSION     DATLBZ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12441
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3563588"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2075)
                     /locus_tag="VMZ53_30825"
     CDS             complement(<1..2075)
                     /locus_tag="VMZ53_30825"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HUS32951.1"
                     /translation="MTDSKKPDDAAKPEDSAASPEQVALDSKVGTSVPEVDEKPAPQV
                     DEKPAPQPWPPVATPIDPDATKQATADDILDEVVLADSSAFGDDFMLADEMVPAVGLE
                     PPAPAGAMVVPIAPVLSSPQEAALEAARIVSQMVDIESESGAVIAAAAAAGAAIEAAV
                     AAQKAAAASAAATAASAEASNEVSPRASAPNLRVSELAAAGATHDPVTTVRLSRDRIL
                     QALASEDIAVPVPPGQRVRARTEPGIDPAADDAYEEFDPVAIAAAAIGRLRQRARTDL
                     AELRVHYHRHDILVLLLAFVIIVIVGQVHKRLVTPPDETFSEHGLTFKHSTAWLKPEP
                     LPLTAPRLVRDASGAQPTTDESLYHVELSAIGSQAKLEVLVDKKPAWSNIVTGLDLDR
                     RSRWGELYSLDESSVRSTAGHDWLRTSYRYAHAEKDDVPRVDHALEYATIDRDQIYVI
                     TIFGSPGEISQIEEVLAPSLRVQSQTALPLVPETGRFAQKKYPNGVGRAFASTVMVVV
                     ADLVDGRLKARGGGSGVVVGAEGSILTNYHVLHDKDGRLHDTFIIGRFGSLDKAPSLQ
                     CAGRPNRSKLLREMDLALIKCDMDLDGRTWSPTEGGVWATLPEARAADIKMGQRLWVL
                     GYPDVGGGGLRWSTGEVEGWTGEDGAQGKDFIKTDASITHGNSGGPVIDDNGRLVGIA
                     AAFRTRMTAT"
     gene            complement(2072..2956)
                     /locus_tag="VMZ53_30830"
     CDS             complement(2072..2956)
                     /locus_tag="VMZ53_30830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HUS32952.1"
                     /translation="MKRPRWSIRRTRILLGTKFLLVAGLVGFVLVCWFAELVAGLHRP
                     VHLGPILAGLMAGIPAALWLGVFYLMDRHEPEPKQLVVGVCVLGALIAAPLADFVISV
                     AVPRVPIEQPDLSALSFDRMLYAVLCAGLAQEMCKYAVVRYTIYMSREFDEPMDGIVY
                     MMACGTGFAVWINYHRLSGQGHNVHLSTGAAQAVVTTLAHASFAGALGYVMGRAKFSR
                     RSAPVRGVLLMGGLLGAALLNGFFGVVTNWIQQSGMAQHPWRGVGWAALCATAVFAVI
                     WFFAQRLLRASPFKDNAA"
     gene            3026..3904
                     /locus_tag="VMZ53_30835"
     CDS             3026..3904
                     /locus_tag="VMZ53_30835"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HUS32953.1"
                     /translation="MAKKQGTDAQLLTIHELAVYLHLDEPTVNKLVAAGKIPAVQLDR
                     QWRFKKASIDEWIEEQLVGDDESFVDVPDGMKLPLEDLLPDQAIIPNLRATTPIAVID
                     ELAARAYTNGWLADKPWFVGAVVERESLSSTAMEGGVAFLHTRAKDKGKIARPFMVVG
                     RSWEGISFGAVDGNPTYLFFLLGLKYDRLHLPILGRLARALRNPATIAKLRSLQSPDQ
                     VRAVLLKEDANVREGLVPEFQGEAFKPKLDRTLRLRAIRRLQAAKKPDPPPAAPKKKS
                     KAASAKAKTAKPAPSK"
     gene            3968..4669
                     /locus_tag="VMZ53_30840"
     CDS             3968..4669
                     /locus_tag="VMZ53_30840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32954.1"
                     /translation="MGRTALYRDPVPYRPARAQVHLDADALQLAMPDGRVRWHRLDGV
                     VATTSDGFVQEPWALPERLARGSSELIPHRHAVPAAPRLRRRFVRMLVIERERDHFVI
                     ITPPDEGAVAPGVVHMPEAPADAAIVEPHTWEALSEWLLAGGRLAACSISDLARLASI
                     ATPQFAVLLGEVAAQRALEYIWAESGPLRGGFDLDSALQPLVEAAKTSPRAGEALVSA
                     LSHAAGARRRRRWAR"
     gene            4706..5041
                     /locus_tag="VMZ53_30845"
     CDS             4706..5041
                     /locus_tag="VMZ53_30845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32955.1"
                     /translation="MRYAIFALAVLAGCQSSDVSRGVGARCDQSSECDERCLLPSADW
                     PGGFCTISCDSDADCPGDAACVDEDGSAICAFTCVTDPGCTFLGQGYVCKERDAHGVA
                     GKASVCRGG"
     gene            5026..5631
                     /locus_tag="VMZ53_30850"
     CDS             5026..5631
                     /locus_tag="VMZ53_30850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32956.1"
                     /translation="MSRWLIIPVLLVGAGVAHADEPLATSTAAAPPDMSDQSIGGSIG
                     VVAGGRTTPGGLVIAGHYLYQLADQDWFDGVAAFTFGGGEGACFRDRMNDFLCEHGLA
                     DGYSIEIAANVRRFLGGNGDFWPFIRAGAGLELVRFGDDGVTGAAIPLHAGGGLRVSV
                     SPDIAITAAAELELGFGLFNHSLGVEPQLGASVSAGAEFRL"
     gene            5703..7229
                     /locus_tag="VMZ53_30855"
     CDS             5703..7229
                     /locus_tag="VMZ53_30855"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HUS32957.1"
                     /translation="MLVVRVTDGDQAVAARVLLFDEHNQPVHIGSIDLYGKRQGAAAC
                     VFDDEVVGSWDGLILGRGIAEVPVGVDACQPSPAIPYGRYHVWAWRGIEYERWEGDVD
                     LREGRGYVSLEIALERAWIPHGTLAADLHVHAHASSDSTMPNPQRVVAQLAAGIQVIA
                     LTDHNTNGDLDAEIQQLEVGNRIASIAGNELTSNNQHVNVYPVPLDRNLPAGGAPPTE
                     TVINATPDQLFGIGRAMKTNPIVQVNHPRFRVGSMYDTAGWDGVKWPPPFPTTFDAVE
                     VLAGYSAFNVEGDRRLDDGVRDLYTFYDHGKLVIPVANSDTHDLNWVLDGTARTYVFT
                     EDTTMSPFDEEGFISALRSRRTVATTGPWLDVEVASKEGATPTVGPGDAIVPDGTTTK
                     SVWLDITLSQTKWVHCERIRITIGSATGPQLVQTIDVPPNVRTHHFAGRIEVGAADTW
                     IGVTADGDTAMPLEFTGTYQKDKWGRAGVTPFALASPILVDADNDGRWKRGDTDLPLR
                     "
     gene            7431..8054
                     /locus_tag="VMZ53_30860"
     CDS             7431..8054
                     /locus_tag="VMZ53_30860"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HUS32958.1"
                     /translation="MEFFVQGWGLTIALCAFIVLVFLAGWLHVHRELAWVRTLTDLLG
                     DDLVDESKPKSPKSLEQQALRDEMHAIKSAKDGGWAQRQVREWQMRAQRLEPVLAFWV
                     DLLRQLGLLGTVLGIGLSLMYTGTDLTKLLGPLALKVWTTVAGLATSIVLSASFAMKL
                     TAWVDAAEKNLEAWDERRLAARARAKMDASGTHSTSTTSNTASEAAS"
     gene            8356..10770
                     /locus_tag="VMZ53_30865"
     CDS             8356..10770
                     /locus_tag="VMZ53_30865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32959.1"
                     /translation="MLFIVIFAALIRAAAVQQAAAQVPAPAKPQPPSPPVKLEVAQLR
                     AQAVSSVSSDIAGRPTVIVRISAAGSVTAIETATATATSPTSPTSPTSPTSPTTATSP
                     TSPTSPTPAPAPLASANTRESTGEGDVKKLLGYDASSSWGDLADSSFDVKLPPTELTP
                     SKSSPRSPSGPPSGPSSGPPSGPSSGTSSGPPDRTPPPKMLQNPWAAPARTGKTGAFP
                     RPPDEAPSTPSVPGAPSSGVGLGDMPDPWNTPSSSSPGAVMPAPAAAESTARQTPSAL
                     AFAGEPPAPPPLSPVDDGIDVDVDMGDSGPAFPASASNASSPTAAVLASASVAVDATL
                     TAHDGGFDPWRGDTIPPMAAPAAASEWRGQAGAELDIPPSPLTQDEMAAALTPRGSPL
                     QGSPFAMPSPSSSTSFDPHGVSPPPSPSPFARSPSKSPFTPPPSPSPFANPPSALPAV
                     QGSADDVMDLDVEVEEPAPIPSWKRSAVESIPELEPRPASLTWPPSSNASPSANASSA
                     DLGVRVRGVGGGGRVDVMPGSPASSSASPAAFPSTAPSQPSPADRTSEWPAREQIRVD
                     DGPVKQSVRYGDDYSSSSSAMPAAAPSAPSPSSFASPKDWPTQSAAAPTRSDPAPGKV
                     LDPWDEIGGSEGALDLDNRAPPTSTPLDSVLGVAVEQKPGGWSTAQLDSVTAPAPEDE
                     CTSLMRGARELFELGDFSGSLDLVEKVLKLEPQNEGARAYMVRNESTLIKMYESKLGN
                     MQKAPKQLMPPDEVIWMNMHHKAGFLLSQVDGSLTYEDLLEVSGMPRFDTMRILHELV
                     LQGIIG"
     gene            10770..11579
                     /locus_tag="VMZ53_30870"
     CDS             10770..11579
                     /locus_tag="VMZ53_30870"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUS32960.1"
                     /translation="MLDHDDDSAVGDELRAATFVKCGIYDAPEWYDVDYAGYRGEEAF
                     YRLVLQRHVKAGGVVVELGVGTGRLALKFAAEGFRIHGVEPAAAMRELLQKKSNDIVV
                     VDGIARDFAKPAGRVDVVMFPFNGLLHIKTREEMMASFRHVRDVLDDDGRFALDCTGP
                     YWDAILYGPMPWGRADERTHHLTGKRVFTCDRSSYDPSTREMRIDIRYLVDGERQGVE
                     IALLQMMWTWQQVLGALEESGFAIDLLYGDVDMAPFDEGSPRLLVSARKRP"
     gene            complement(11583..>12441)
                     /locus_tag="VMZ53_30875"
     CDS             complement(11583..>12441)
                     /locus_tag="VMZ53_30875"
                     /inference="COORDINATES: protein motif:HMM:NF014395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32961.1"
                     /translation="CVRSMTMSDTVSTANSTGLDYHPTPEELRPHKLYEVQHWRDSTS
                     RYLDGKTFGYEATIPPLTQEQFRELPSATYHAVVGAVAGGLAGERIAFECSAALCAYA
                     PDDNARMFLATQVIDEARHVEVFTHRLRLLGREDIDDTLRRSVNPRMFKFHDTLRERV
                     CEARDFVGGVIGQNLALEGLALGFFEFNASLLEDVDPGTSQILKTVLLDERRHVGFGL
                     MRLRGLLDAQPDKRAIVEDTVGELSDEMMAIFEENADNMARLGVDPSAPMTKVRSYHH
                     MHLAKLGLH"
BASE COUNT         1997 a         4209 c         4231 g         2004 t
ORIGIN      
        1 gttgcagtca tgcgcgtgcg aaatgccgcc gcaatcccga cgagccggcc gttgtcgtcg
       61 atgacgggac cgcctgagtt gccgtgcgtg atcgatgcgt cggtcttgat gaagtctttg
      121 ccctgcgcgc catcctcgcc ggtccagccc tcgacttcgc cggtcgacca gcgcaggccg
      181 ccgccgccga cgtcgggata tccgagcacc cagaggcgct gacccatctt gatgtccgcc
      241 gcgcgcgctt cggggagcgt cgcccacacg ccgccttcgg tcggcgacca cgtacggccg
      301 tcgagatcca tgtcgcactt gatcagcgcg agatccattt cgcgcagcag cttgctgcga
      361 ttcggccgac cggcgcactg gagcgacggt gctttgtcga gcgagccgaa gcgaccgatg
      421 atgaacgtgt cgtgcaggcg tccgtccttg tcgtggagga cgtgatagtt cgtgaggatc
      481 gagccttcgg cgccgacgac gacgcccgag ccgccaccgc gcgctttgag cctgccgtcg
      541 acgaggtcgg cgacgacaac catcaccgtc gaagcgaacg cgcgaccgac gccgttggga
      601 tacttcttct gcgcgaaccg tccggtctcc ggcacgagtg gcagcgcggt ctgcgattgc
      661 acgcgcagcg agggtgcgag cacctcttcg atctgtgaaa tctcgccagg cgaaccgaag
      721 atcgtgatga cgtaaatctg atcgcgatcg atcgtcgcgt attcgagcgc atggtcgacg
      781 cgcggcacat catctttctc cgcgtgcgca tagcgatatg acgtgcgcag ccaatcgtgg
      841 ccggcggtcg aacgcacgct gctctcgtcg aggctgtaca gctcgcccca gcgcgatcgg
      901 cgatcgagat cgaggccggt gacgatgttc gaccacgcag gcttcttgtc gacgagcact
      961 tcgagcttcg cctgcgatcc gatcgccgag agctcgacgt gatagaggct ctcgtcggtc
     1021 gtcggctgtg caccggacgc atcccgaacg aggcgcggcg ccgtcagcgg cagaggctct
     1081 ggcttgagcc acgccgtcga gtgcttgaac gtcaggccgt gctcggagaa cgtctcgtcg
     1141 ggcggcgtga cgaggcgctt gtgcacctgg ccgacgatca cgatgatcac gaacgcgagc
     1201 agcagcacga gaatgtcgtg gcggtggtag tggacgcgca gctcggcgag atccgtgcgt
     1261 gcgcgctggc gcaaccgacc gatcgctgcc gccgcgatcg cgacgggatc gaactcttcg
     1321 tacgcgtcgt cggcagctgg atcgatgccg ggctctgtgc gcgcacgaac gcgttgaccg
     1381 ggcggcacgg ggaccgcgat gtcctccgat gcgagcgctt gcagaatgcg atcgcgcgag
     1441 agccgcaccg tcgtgacggg gtcgtgcgtg gcgccggccg cagcgagctc gctcacgcgg
     1501 agattcggcg ccgatgcgcg cggcgagact tcgttcgagg cttcggccga tgcggcggtc
     1561 gcggcggcgc ttgcagcggc agccttctgc gcggccacgg cggcttcgat cgcagcaccg
     1621 gcggcggcgg ccgcggcgat cacggcgccg gactcgctct cgatgtcgac catctggctg
     1681 acgatgcggg ccgcttcgag cgcggcctcc tgcggcgatg acaggaccgg cgcaatcggg
     1741 acgaccatgg cgcccgctgg agcgggtggc tcgagcccga ccgcaggcac catctcgtcg
     1801 gcgagcatga agtcgtcgcc gaacgcgctc gaatccgcga gcacgacttc gtcgaggatg
     1861 tcgtcggcgg tcgcctgctt cgtcgcgtcg gggtcgatcg gcgttgcgac cggaggccac
     1921 ggttggggag ctggcttttc gtcgacttgg ggagctggct tttcgtcgac ttccgggacc
     1981 gacgtgccga ccttcgagtc gagcgcgact tgctcgggac tcgcagcgga gtcttcgggc
     2041 ttcgccgcgt cgtcgggttt cttcgagtcg gtcacgcggc gttgtccttg aacggcgatg
     2101 cgcgaagcag tcgctgcgcg aagaaccaga tcaccgcgaa cacggcggtc gcgcagagcg
     2161 ctgcccagcc gacgccgcgc caggggtgct gcgccatgcc gctctgctgg atccaattcg
     2221 tgacgacccc gaagaagcca ttgagcagcg cggccccgag caggccgccc atgagcagca
     2281 cgccacgaac cggtgcgctg cgtcgcgaga acttcgcgcg tcccatcacg tagccgagtg
     2341 cgccggcgaa cgatgcgtgg gcaagcgtcg tgacgacggc ttgggccgcg cccgtcgaga
     2401 gatggacgtt gtgaccttgg ccgctgaggc ggtggtagtt gatccagacc gcgaagccgg
     2461 tgccgcacgc catcatgtag acgatgccgt ccatcggctc gtcgaattcg cgcgacatgt
     2521 agatcgtgta gcggacgacg gcgtacttgc acatctcctg cgcgaggccc gcgcagagga
     2581 ctgcatagag catgcggtcg aatgacagcg ccgagagatc gggctgctcg atcggtacgc
     2641 gcgggaccgc gacggagatc acgaagtcgg cgagcggcgc ggcgatgagc gcgccgagga
     2701 cgcagacgcc gacgacgagc tgcttgggct cgggctcgtg gcgatccatc agatagaaga
     2761 cgccgagcca cagcgcggcg gggatgccgg ccatcaggcc cgcgaggatg gggcccaggt
     2821 ggacaggacg atggagcccc gcgacgagct ccgcgaacca gcacacgaga acgaacccga
     2881 cgagccctgc aacaagcagg aacttcgttc cgagcaggat ccgggtccgg cgaatcgacc
     2941 aacgaggcct tttcaccccg aaattctacg gcacacgagg atggcgcgcg cgagatcggc
     3001 cttctagaat gcgcgccagc gctacatggc caaaaagcaa ggaactgacg cacagttgct
     3061 gacgatccac gagctcgcgg tgtacctcca tttggacgag ccgaccgtaa acaagctcgt
     3121 ggccgccgga aagatcccag cggtccagct cgatcggcag tggcgcttca agaaggcctc
     3181 gatcgacgag tggatcgaag agcagcttgt tggcgacgac gagagcttcg tcgacgtgcc
     3241 cgacggcatg aagctgccgc tcgaagatct gctgccggac caagcgatca ttccgaacct
     3301 ccgcgcgacg acgccgatcg cggtgatcga cgagcttgcc gcgcgcgcgt atacgaatgg
     3361 ttggctcgcc gacaagccgt ggttcgtcgg tgcggtcgtc gagcgcgagt cgctgtcgtc
     3421 gacggcgatg gaaggcggcg tcgcgttcct gcacacgcgt gcaaaggaca agggcaagat
     3481 cgccaggccg ttcatggtcg tcggccggtc gtgggaaggg atttcgttcg gcgccgtcga
     3541 cggcaatccg acgtatctgt tcttcctgct cggcctcaag tacgaccggc tgcacctgcc
     3601 gatcctcggc cggctcgcgc gtgcgctccg caacccggcc accatcgcga agcttcgctc
     3661 gttgcagtcg cccgaccagg tgcgcgcggt cctcctcaaa gaggatgcaa acgtccgtga
     3721 gggcctcgtc cccgagttcc agggtgaggc attcaagccc aagctcgacc gcacgttgcg
     3781 cctgcgcgcg atccgccggc tccaagcggc aaagaagccc gatccgccgc cggccgcgcc
     3841 caagaagaag tccaaggccg cgtccgctaa ggcgaagacc gccaagccag caccgtcgaa
     3901 gtgaccggcg cacgctgccc gcgagttgcc tagacaactt tcgggagtgc actgctacat
     3961 tcttattgtg ggccgaactg cgctgtatcg ggatccggtg ccctaccgac ccgctcgcgc
     4021 ccaagtccac ctcgacgccg acgcgctcca gctcgcgatg cccgatggcc gcgtgcgctg
     4081 gcaccgcctc gatggtgtcg tcgccacgac gagcgatggc ttcgtgcaag agccgtgggc
     4141 gctgccggag cggctggcgc gtggcagctc ggagctgatc ccacaccggc atgcggtccc
     4201 ggccgcgccg aggttgcgtc gacgattcgt gcgcatgctc gtgatcgaac gcgagcgcga
     4261 tcacttcgtc atcatcacgc cgcccgacga aggtgcggtg gctccgggcg tcgtgcacat
     4321 gccggaggcg cctgccgatg ccgcgatcgt cgagccgcac acgtgggaag cactgtccga
     4381 gtggctgctc gcgggcgggc gtctcgctgc gtgctcgatt tcggatctcg cgcggctcgc
     4441 gagcatcgca acgccgcagt tcgcggtgct cctcggcgag gtcgctgcgc agcgcgcgct
     4501 cgaatacatc tgggccgaga gcggtccgct gcgtggtggc ttcgatctgg attctgcgct
     4561 gcagccgctt gtcgaagctg cgaagacgtc gccgcgtgcg ggtgaggcgc tcgtttcggc
     4621 gctttcgcat gcggctggcg cgcgacgtcg tcgtcggtgg gctcgatagt ggcatccgcg
     4681 atctgatggc ggtagcttcg cggccatgcg gtacgcgatc ttcgcgctcg cagtgttggc
     4741 gggatgtcag agctcggacg tctcgcgcgg cgttggtgcg cgctgcgatc agagctcgga
     4801 gtgcgacgag cgctgcctcc tgccgagcgc tgactggccg ggcggcttct gcacgatctc
     4861 gtgcgacagc gatgccgatt gcccaggcga tgccgcgtgc gtcgacgagg atggcagcgc
     4921 gatctgcgcg ttcacgtgcg tgaccgatcc aggctgcacg tttctcggcc aaggctacgt
     4981 atgcaaggaa cgcgatgcgc atggcgtcgc cggaaaggcc agcgtatgtc gcggtggctg
     5041 atcatcccgg tgcttctcgt cggtgccggt gtcgcgcacg ccgacgagcc gctcgccacg
     5101 tcgactgccg cggcgccgcc tgacatgagc gatcagagca tcggcggcag catcggcgtc
     5161 gtcgccggtg gacgcaccac gccgggcggt ctcgtcatcg ccggccacta cctctatcag
     5221 ctcgccgacc aagactggtt cgacggcgtt gcagcattca cgttcggcgg cggcgaaggc
     5281 gcgtgctttc gcgatcgcat gaacgatttc ctctgcgagc acggcctcgc cgacggctac
     5341 tcgatcgaga tcgctgcaaa cgtccgccgc ttcctcggtg gcaacggcga tttctggccg
     5401 ttcattcgcg cgggcgcggg gctcgagctc gtgcgtttcg gcgacgacgg cgtcaccggc
     5461 gctgccattc cactccacgc gggcggtggc ctgcgcgtct cggtctcgcc cgacatcgcg
     5521 atcaccgcgg ctgcagagct cgagctcggc ttcggcctct tcaaccattc gctcggcgtc
     5581 gagcctcagc tcggagcaag cgtctcggca ggcgccgaat tccggctgtg attcgctggc
     5641 tgctgatcgc gcttgtcgct gctgtagcct gcgggaagcc gtcgcgaccg aaatcgacga
     5701 cgatgttggt cgtacgcgtc accgacggtg accaagccgt cgccgcgcgc gtgctgctgt
     5761 tcgacgagca caaccaaccc gttcatatcg gctcgatcga cctctacggc aaacgccaag
     5821 gcgcggctgc gtgcgtgttc gatgacgaag tcgtcgggtc gtgggacggc ctcatccttg
     5881 gccgcggcat cgctgaggtt cccgtgggtg tcgacgcgtg ccagccatcg ccggcgatcc
     5941 cgtacggccg ctatcacgtg tgggcgtggc gcggcatcga gtacgagcga tgggaaggcg
     6001 acgtcgatct ccgcgaaggc cgcggctacg tctcgctcga gatcgcgctc gaacgcgcgt
     6061 ggatcccgca cggcacgctg gccgcggacc tccacgtcca cgcgcacgcg tcgagtgatt
     6121 cgacgatgcc caatccgcaa cgcgtcgtcg cgcagctcgc ggcgggtatc caagtcatcg
     6181 cgctcaccga ccacaacacg aacggcgatc tcgacgccga gatccagcag ctcgaagtcg
     6241 gcaatcgcat cgcttcgatc gcaggcaacg agctgacgtc gaacaaccag cacgtcaacg
     6301 tctaccccgt gccgctcgat cgcaacctgc ccgcaggtgg cgcgccgccg accgagaccg
     6361 tcatcaacgc gacgcccgac cagctgttcg gcatcggccg cgcgatgaag acgaacccga
     6421 tcgtgcaggt caaccacccg cggttccgcg tcggctcgat gtacgacacc gccggctggg
     6481 acggcgtcaa gtggccgcca ccgttcccga cgacgttcga cgccgtcgag gtgctcgcgg
     6541 ggtacagcgc gttcaacgtc gagggtgatc gccgcctcga cgacggcgtg cgcgacctct
     6601 acacgttcta cgaccacggc aagctcgtca tcccggtcgc caactccgac acgcacgatc
     6661 tcaactgggt gctcgacggc acggcgcgca cgtacgtctt caccgaggac acgacgatgt
     6721 ccccgttcga cgaggaaggc ttcatcagcg cgctgcgctc gcggcgcacg gtcgcgacga
     6781 caggtccatg gctcgacgtc gaggtcgcgt cgaaggaagg cgcgacgccg acggtggggc
     6841 cgggcgatgc gatcgtgccc gacggcacga cgacgaagag cgtgtggctc gacatcacgc
     6901 tgtcgcagac gaagtgggtg cactgcgagc gcatccgcat cacgatcggc tcggcgacgg
     6961 gcccacagct cgtgcagacg atcgacgtgc cgccgaacgt gcggacgcat cactttgccg
     7021 gacgcatcga ggtcggcgcc gcggacacgt ggatcggcgt caccgccgac ggcgacacgg
     7081 cgatgccgct cgaattcacg ggcacatatc agaaagacaa atggggccgc gctggcgtca
     7141 cgccgttcgc gctcgcctcg ccgatcctcg tcgacgccga caatgacggc cgctggaagc
     7201 gcggcgacac cgacctgcca ctgcgttgag tccgtgcaaa acgcgttgtc gacgcgcata
     7261 tcgctgaacg cgcatatcgc tgcacgcgca tatcgccgaa acgcgcgcat cgctgaaacg
     7321 cgcgtatcgc ggcgatgacg cagcctggcg gcttgcaaac gtggtcgcgc tgacgtctcg
     7381 cgtcgcggtt gacgatgtag gtcccgcgct cgcgttatga tttgccggcg atggagtttt
     7441 tcgtccaggg gtggggcctc acgatcgcgc tctgcgcgtt catcgtgctg gtgttcctcg
     7501 cgggctggct gcacgtccat cgcgagctcg cgtgggttcg cacgctgacg gacctgctcg
     7561 gcgatgacct cgtcgacgaa tcgaagccca aatcgccgaa gtcgctcgag cagcaagcgc
     7621 tgcgcgacga gatgcacgcg atcaagtcgg ccaaagacgg cggctgggcg cagcgccaag
     7681 tccgcgagtg gcagatgcgt gcgcagcgcc tcgagccggt gctcgcattc tgggtcgact
     7741 tgctgcgcca gctcggcctg ctcggcacgg tgctcggcat cggcctatcg ctgatgtaca
     7801 cgggcaccga tctgacgaag ctcctcggcc cgctcgcgct caaagtgtgg acgacggtcg
     7861 cgggtctcgc gacgagcatc gtgctgtcgg cgtcgttcgc gatgaagctc acggcgtggg
     7921 tcgacgcggc ggagaagaat ctcgaggcgt gggacgaacg gcgcctcgcg gcgcgcgcac
     7981 gcgccaagat ggatgcgtcg ggcacgcact cgacgagcac gacgagcaac accgcgtcgg
     8041 aggccgcgtc gtgagcgcgc atgcgatgag ctcgtcgggc ggcgtcgtgg tcgcgcgctc
     8101 gttggaggcg gcgtcgtgac cgcgcacgcg atgagcacgt cgggcggcgt cgtggtcggg
     8161 cgcgcgtcgg aggccgcatc gtgaccgcgc actcgacgag cacgacgagc aacaccgcgt
     8221 cggaggccgc atcgtgaccg cgcacgcgac gagctcgtcg ggcggcgtcg tgatcgcgcg
     8281 ctcctcggag gccacgtcgt gagccggcga tcgcgtcgtc gtcagccgcc gccggatcta
     8341 acgtcgctgt tcgacgtgct gttcatcgta atcttcgcgg cgctcattcg cgcagctgcg
     8401 gtgcagcaag ccgctgcgca ggtgcccgca ccggccaagc cacagccgcc gtcgccgccc
     8461 gtgaagctcg aggtcgcgca gctccgcgcg caggccgtga gctccgtgag cagcgacatc
     8521 gcgggacgcc cgacggtcat cgttcgcatc agcgcggcgg gctcggtgac ggcgatcgag
     8581 accgcgacgg caacggcgac gagcccgacg agcccgacga gcccgacgag cccgacgagc
     8641 ccgacgaccg cgacgagccc gacgagcccg acgagcccga cgcccgcgcc tgcgccgctc
     8701 gcgtccgcaa acacgcgcga gtcgacgggc gagggcgacg tgaagaagct cctcggctac
     8761 gacgcgtcgt cgagctgggg cgatctcgct gactcgtcct ttgacgtgaa actcccgccc
     8821 accgagctca cgccgtcgaa gtcgtcgccg cgctccccga gcggaccgcc gagcggaccg
     8881 tcgagcggac cgccgagcgg accgtcgagc ggaacgtcga gcggaccgcc tgatcggacg
     8941 ccgccgccga agatgctgca gaacccgtgg gcggcccctg cgcgcaccgg caagacgggc
     9001 gctttcccgc gtccgcccga tgaggcgccc agcacgccca gcgtccccgg cgccccctct
     9061 tccggcgtcg gtttgggcga catgccggac ccgtggaaca cgccgagctc gtcgagcccc
     9121 ggcgccgtga tgcccgcgcc cgcggccgcc gaatccacag cgcgccagac gccgtcagcg
     9181 ctcgcatttg ccggcgagcc gcccgcgccg ccgccgctct cgcccgttga cgacggcatc
     9241 gacgtcgacg tcgacatggg ggacagcggg cccgcctttc ccgcgtcggc atcgaacgca
     9301 tcgtcgccca cggccgccgt gctcgcgagc gcatcggtcg ccgtcgacgc cacgctcacc
     9361 gcacacgacg gcggcttcga tccttggcgc ggcgacacca tcccgcccat ggcagccccc
     9421 gccgcagcca gcgagtggcg cgggcaggcg ggcgcggagc tcgacattcc tccatcgccg
     9481 ctcacgcaag acgagatggc cgccgcgctc acgccgcgtg gcagtcccct gcagggctcg
     9541 ccgtttgcga tgccgtcccc gtcgtcgtcg acgtcattcg atccgcacgg tgtctccccg
     9601 ccgccgagcc cctcgccgtt tgcccgatcg ccgagcaagt cgcccttcac gccgccgccg
     9661 agcccctcgc cgtttgccaa tccgccctcc gcgctcccgg cggtgcaggg atcggctgac
     9721 gacgtcatgg acctcgacgt cgaagtggag gagcccgccc ccatcccatc gtggaagcgc
     9781 tcggcggtgg agtccatccc ggagctcgag ccgcgtccgg cgtcgctgac gtggccgccg
     9841 tcgtcgaatg cctcaccttc ggcgaatgcg tcgtcggccg atctcggcgt gcgtgttcgc
     9901 ggcgtgggtg gcggcggtcg tgtcgacgtc atgccgggct cgcccgcttc gtcgtcggct
     9961 tcgcctgccg ctttcccgtc gacggctccg tcacaaccgt cgcccgccga tcgcacgtcg
    10021 gagtggccgg cgcgcgagca aatccgcgtc gacgatgggc cggtgaagca gtcggtgcgc
    10081 tacggagatg actactcgtc gtcgtcgtca gcgatgcccg cagccgcgcc gagcgccccg
    10141 tcgccttctt cgtttgcgtc gcccaaggac tggcccacgc aaagcgcggc cgcgccgacg
    10201 cgcagcgatc ctgcgccggg caaagtgctc gatccatggg acgagatcgg cggctcggaa
    10261 ggcgcgctcg acctcgacaa ccgcgcaccg cccacgtcca cgccgctcga cagcgtgctc
    10321 ggagtggcag tcgagcagaa gccgggtggt tggtccacgg cgcagctcga ctcggtgact
    10381 gcaccggcgc ccgaagacga atgcacgtcg ctcatgcgcg gcgcgcgcga gctgttcgag
    10441 ctcggtgact tctcgggctc gctcgacctc gtcgagaagg tgctcaagct cgagccgcag
    10501 aacgaaggcg cgcgcgcgta catggtgcgc aacgagagca cgctcatcaa gatgtacgag
    10561 tcgaagctcg gcaacatgca gaaggccccg aagcagctca tgccgcctga cgaggtcatc
    10621 tggatgaaca tgcaccacaa ggcgggcttc ttgttgtcgc aggtggacgg ctctttgacg
    10681 tacgaggatc tcctcgaggt ctcgggcatg ccgcgcttcg acaccatgcg catcctgcac
    10741 gagctcgtgt tgcagggcat catcggctga tgctcgacca cgatgacgac agcgccgtcg
    10801 gcgatgagct gcgcgccgcg acgtttgtga agtgtggcat ctacgacgcg cccgagtggt
    10861 acgacgtcga ctacgcgggc tatcgcggcg aagaagcgtt ctatcgcctc gtgctgcaac
    10921 gccacgtgaa agcgggcggc gtcgtcgtcg agctcggcgt gggcaccggc cgtctggcgt
    10981 tgaagttcgc ggccgagggc tttcgcattc acggcgtcga gcctgcggcg gccatgcgcg
    11041 agctcttgca aaagaagagc aacgacatcg tcgtcgtcga cggcatcgcg cgcgacttcg
    11101 ccaagcccgc tggccgcgtc gacgtcgtca tgtttccgtt caacggcctg ctgcacatca
    11161 agacgcgcga agaaatgatg gcgagctttc ggcacgttcg ggacgtgctc gacgacgacg
    11221 ggcgctttgc gctcgactgc accggcccct attgggacgc catcctctat gggcccatgc
    11281 cgtggggcag ggcagacgag cgcacccatc acctgacggg caagcgcgtg ttcacgtgcg
    11341 atcgctcttc gtacgatccg tcgacgcgcg agatgcgcat cgacatccgc tacctggtcg
    11401 acggtgaacg gcaaggcgtc gagatcgcgc tcttgcaaat gatgtggaca tggcagcaag
    11461 tgcttggcgc gctcgaggag agcggctttg ccatcgactt gctctacggc gacgtcgaca
    11521 tggcgccgtt cgatgaaggc tcaccgcgct tgctcgtgag cgcgcgcaag cgcccttgag
    11581 gatcagtgga ggccgagctt cgccaggtgc atgtggtgat agctgcggac cttggtcatc
    11641 ggtgccgacg ggtcgacgcc caaacgcgcc atgttgtccg cgttctcttc gaagatggcc
    11701 atcatctcgt cgctcagctc gccgaccgtg tcctcgacga tggcgcgctt gtccggctgc
    11761 gcatcgagca gtccgcgcag acgcatcaat ccgaagccca cgtggcgccg ttcatcgagc
    11821 aggacagtct tcaagatctg cgacgtaccc gggtcgacgt cttcgagcag cgacgcgttg
    11881 aactcgaaaa atccgagcgc gaggccctcg agcgcgaggt tctgcccgat gacgccaccg
    11941 acgaagtcgc gcgcttcaca cacgcgctcg cgcagggtgt cgtgaaactt gaacatgcgc
    12001 gggttgacgg agcgacgaag ggtgtcgtcg atgtcctcgc gccccaacag gcgcaggcgg
    12061 tgcgtgaaca cctcgacatg gcgcgcctcg tcgatgactt gcgtcgcgag gaacatgcgg
    12121 gcgttgtcgt cgggcgcata tgcacagagc gcggcgctgc actcgaaggc aatgcgctca
    12181 ccggcgagac caccggccac agcgcccacc accgcgtggt acgtcgccga cggcagctcg
    12241 cgaaactgct cctgcgtgag cggcgggatg gtggcctcgt agccgaacgt cttgccgtcg
    12301 aggtagcgcg acgtggagtc acgccagtgc tgcacttcat agagcttgtg cggccgcagc
    12361 tcctcgggcg tggggtggta gtcgaggccg gtgctgttcg cggtggagac agtgtcgctc
    12421 atggtcatgg acctcacgca g
//
LOCUS       DATLBZ010000630        10554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10161437, whole genome shotgun sequence.
ACCESSION   DATLBZ010000630 DATLBZ010000000
VERSION     DATLBZ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10161437"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            521..2497
                     /locus_tag="VMZ53_30880"
     CDS             521..2497
                     /locus_tag="VMZ53_30880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HUS32962.1"
                     /translation="MDRSFALTPGKRVLYLTKDPERIRQQLAGTLTLRMADLHPDELL
                     DDINTDAMTPAWVCFDFDPKDIARNAYAGLIVNGERLVSAGALADGGFEVIVAGAQKG
                     VGSSREQAAQCEVFSGSRLAIASSFAPIHARNNINIGQLMGDHAMIARLEAGESVSAA
                     EFTRGHDAITALIIESGGLLPFCARLGRKEISVPAPGTAPRAMNITEKILAAKLLPGQ
                     GTHVKPNDAVLVRVDAGYSHEFTTAQVDKFLTDEYGEYKLQNPAKFAVFEDHLIYATG
                     VASMAKFADKIEKLRVLQRAFAEKTGVRNYSAKNGVSPGICHQVAREQFIDPGDLVQA
                     TDSHTCMGGASGALAWGVGATEYAALLYWGFTPLAVPESIRFELTGRLPAGTTAKDVM
                     LHILATYAKREDTLNRVMEFGGDGLFALSPDERATLANMATECSARGAVMEVDETMLA
                     WMAERRPGVNVDDLRAKAVFPDNGAHYDGGVHRIDLSTIRPMVATPGDPDRGIASDPK
                     NGALVKEIGRVKLDIAYGGSCTAGKRDDVDMYARVMREAEAAGKRVPEGVKFFIQFGS
                     QEVEAYARAQGYIDLFQRTGVEVIKPGCGACIGCGPGVSERGDQVTVSAINRNYKGRS
                     GPGKLYLASPLTVAASAVAGEIVEYRDGMFGPKK"
     gene            2533..3864
                     /locus_tag="VMZ53_30885"
     CDS             2533..3864
                     /locus_tag="VMZ53_30885"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HUS32963.1"
                     /translation="MAKARKKPAKAVPKAKKKPAAKKPAPKTRAAAKKPGAKKAAAKK
                     PAAKRTKKAAPKKAAPKKAAPKRTRVVEPTVEAIPQEVISEPSTGGTLRTLTARERAM
                     RSLPIVEILDDPHPIGALRRFLDSIRGEATQQQAQIALGSAQLMLLPIAREHRGGADV
                     KELIDLVLQHWTGLGDRRKGYHAQEFLRNALAAIGVDRERIAKLEDLVPANATGELLF
                     NLAGAHAVARDKVAMLRAVERALEAGVSSSRFRHDSDFLPYTKDPDLGRLLSRAEVPP
                     IPVDVEPYLPAVRDALDMLVGTLREYGESIELRPPVRLDAILDAERARKISLPNDYRA
                     LLTITNGMALWEHAFFSVGDYRDRTPLAARAQAFIDMMAGNGREGIEDCVPLANWGQP
                     NDWLLYDRRGTLRGNEPGYVLMLNADEHALGDLAAALHHLEFIARETLGTN"
     gene            4005..6758
                     /gene="lon"
                     /locus_tag="VMZ53_30890"
     CDS             4005..6758
                     /gene="lon"
                     /locus_tag="VMZ53_30890"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HUS32964.1"
                     /translation="MELPIVPLRTMVLFPHAALPMTLNRPGASQAVSAAGEEGLLAVV
                     TQANPDVEMPSGRDLYAIGTAAVVRQMAQPGTDTVMVVLEGLERIAIVEVVQTHPYLR
                     ARVRRLTSEKPLSDSEYAAAKLNLLEAFSEMVQLSHVLPDELSVVAKSLSDDAALTDM
                     IGSALVEVPPPTRQDILATLNVRRRMDKLNEVVARELQSLRLREKLKTEVQTKIGETQ
                     KEIVLREQLKAIKKELGDEHGDDRHVTQLKSRLHRGELNEEAMREAERELERLEEMSA
                     ASPEHSMVRTYVEWLAELPWVETTANEIDLKRARQVLDEDHFDLEKVKERILEFLAVQ
                     RLKRSQRGPLICLAGPPGVGKTSIGRSIARATGRKFVRICLGGTNDEAEIRGHRRTYM
                     GSLPGQIIRGLRRAGSCDPVFMLDEIDKIGRDFRGDPAAALLEVLDPEQNNAFRDHYL
                     DVPFDLSKVMFIATANVLDTIPEPLRDRMEIIDLPGYVDEDKVQIAKRYLVPKQVEEN
                     GLVLGEDIEFTDDALFELVHGHTHEAGVRKLEQEIAHVARKRARQLAEGDQGKLIVNR
                     GVVTGFLGQPKYKIENQVEERTRRPGVAVALAWTSQGGELLYVESSKLPNGRGDVTLT
                     GQMGDVMQESARTAVSWLRGHARRYGIAEDVFKKSDIHIHVPAGAVPKDGPSAGIVMI
                     TSLVSLLTNRPIRPFTAMTGEITLSGPLLPIGGLKEKVLAARRSGIKRIIIPLENEPN
                     LHEEVPAHVRGDLEIIFASTMEQAVDLALTPDGGVLMAQQKIQDVMTKVVHCVGENAT
                     VREVARKMRDECIGDVLVTDAQGKLSGIITDRDVVVRAVAEGGDLDSLRAKDICTDHI
                     VTLDPSSGVEDAVKLMTEKAIRRIPVVENDVPIGIVTIGDLAAQREPASALGQISTAA
                     PNN"
     gene            complement(6755..6976)
                     /locus_tag="VMZ53_30895"
     CDS             complement(6755..6976)
                     /locus_tag="VMZ53_30895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32965.1"
                     /translation="MDLRNTRARLKSIAVAAVIGALFTFFTMKMIAGGGGRGPNNDPI
                     AGATFGVLVIFVFVIETVIAHKVISKKRR"
     gene            complement(7003..7566)
                     /locus_tag="VMZ53_30900"
     CDS             complement(7003..7566)
                     /locus_tag="VMZ53_30900"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HUS32966.1"
                     /translation="MRVRIHASEARSRANGPGSRFVVWMQGCTLGCAGCFNPTTHDAL
                     GGREVSIDELVAEMARARDAGSEGLSLSGGEPLQQAEAAAALLDAARALGLTTLAFSG
                     YTVDEIRALPHGPDVLARLDVLIDGRYVSTERLASGLRGSANQRIQLLTDRYAMSDIE
                     ATPVAEIRISATGEAILTGVNPLKLRR"
     gene            complement(7570..9477)
                     /locus_tag="VMZ53_30905"
     CDS             complement(7570..9477)
                     /locus_tag="VMZ53_30905"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HUS32967.1"
                     /translation="MKRLVVLLAAGLSFGPSIAHGKKIPEQLGPPSKAVTDEIIDAIH
                     EEMNRAMAKLEIPGAPKPYSIMYKITEVDVNDVSATLGQTTSRRNRHFVSVEARVRVG
                     NKQFDNGNFVVPDGAELDGTATANLPLEATPRIARRAAWLVTDQAYKEALIQLRAKIE
                     ARRAGGTSHPDVPAWTDEKPVVGEDPVLVAPLETLDDLEARAKKLSAMFRDQPDLRDS
                     RVALTSYLERRWYITTENTSVTDTRRASGIVIEVEGQAPDGQLLNNYFLRYGHTAKDL
                     PSDKELDTESKNLAASISALAKAPLLDKYSGPVLFEGEGAVGLLRFALAPHLGGTPVP
                     EGLSPQEAKSFGGALTDKVTLRVLANNLSIVDDPTAQQGGGKALIGGYKIDDEGVAAQ
                     KVEVIKDGTLKTLLLSRTPSDKKQTSNGHARRAADGGAFHGSATNLFVTAKGGVPRAS
                     LRSKLISAARAEGLKYGLVVRRFDDAAVTGAPEFTRRELIQLLKTTDQELPPPTLLAY
                     KVTADGKEELVRGVQLTDVPIRAWKDVIGVSKEVTVYNFLAASEPQLQLRLTGGTDDG
                     FVPSGGIESSIITPDLLLKEIDIIGQTGNVRPPPAAPKGVVAAPAAAAKGDDKKADDK
                     KPDDKKPAPKK"
     gene            complement(9474..>10554)
                     /locus_tag="VMZ53_30910"
     CDS             complement(9474..>10554)
                     /locus_tag="VMZ53_30910"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HUS32968.1"
                     /translation="TCGVLFQLNTAYYVNSEGSQIQLSWTNAQLSVSVGVKADDGENL
                     SRLEQRFGRTPADLPSDGEVDKMIKEVTNDLDALHDAPLADPYVGPAILQGRASGVFF
                     HEVFGHRIEGHRQKDTTSGRTFASYVGKDIAPEWLSVYDDPTIVTLNGVQLNGFFRYD
                     DEGVKAQHVPLVDKGKLVGFLMGRNPIPGFAHSNGHGRHSPGLPPVARQGNLVVETTR
                     SLPYADLEKMLIEEIKKQKRPYGMIFTDISGGFTNTSAFAPQAFKVNPVMAYRLFPDG
                     KKELVRGIDISGTPLVALQSIRAASREIETFNGVCGAESGWVPVSASAPSLLIERLEV
                     EKGFIPPDRPPLLDAPTSIKQKVSP"
BASE COUNT         1797 a         3405 c         3525 g         1827 t
ORIGIN      
        1 gactcgccgc cggccccggg cgcggctcgc tccatttcct ctgcgcctta cgagctcgat
       61 ctcgcggagc cgtgcccacg tgggcacgaa catccgagcg cggttcgctc cattttcgtc
      121 agcgcgtcga tcgacatggc ggcgccgtgc ccacgggggc acgctcagat gttcgcgtcg
      181 cgcgtccact tcgcgacggc cggcgtcgac actgccacga cgtcgataaa cgttctcggt
      241 tgatgtcgga cgagctcgat catgcggcgc cgtgcccacg tcggcacgct cggctgttcg
      301 cgtcgggtgt cgccgacggt accgtgccca cgtgggcact cgtgaatcga gacggcgctg
      361 cgcttttctt cgacgacggc tgcgtgttct tcctgtcacg taagccacat ccccgggtgc
      421 gctcgcggcg ggcggcgctg cgcgaattgc gcacctcgtc attttcggtc gtgcgttggg
      481 ctcgatcacc ggggcgcgga gggtggtacg gttcgccccg atggatcgca gtttcgcgct
      541 gaccccgggc aagcgcgtcc tctatctgac gaaggatccc gagcggattc gccaacagtt
      601 ggcgggcacg ttgacgctcc gcatggcgga ccttcatccc gacgagctcc tcgacgacat
      661 caatacggat gcgatgacac ccgcgtgggt gtgctttgac ttcgatccga aggacattgc
      721 gcggaatgcg tatgccggct tgatcgtgaa tggcgagcgg ctggtttcgg ccggtgcgtt
      781 agcggatggc ggcttcgagg tgattgttgc tggcgcgcag aagggcgttg gctcgagtag
      841 agagcaggcg gcgcaatgcg aggtctttag tggcagccgg cttgccattg cgagctcgtt
      901 cgcgcccatt cacgcgcgca acaacatcaa catcggacag ctgatgggcg accacgcgat
      961 gatcgcgcgg ctcgaagctg gcgagagcgt ttccgcggcg gagttcacgc gcgggcatga
     1021 tgcgattaca gcgctgatta tcgagagcgg cgggctgctg ccgttttgcg cgcggctcgg
     1081 gcggaaagag atcagcgtgc cggcgcccgg gactgcgccg cgggcgatga atatcaccga
     1141 gaagattttg gccgcgaagc tgttgcctgg acagggcacg cacgtgaagc cgaatgatgc
     1201 agtgctcgtg cgcgtcgacg cggggtactc gcacgaattc acgaccgcgc aggtcgacaa
     1261 gtttctcacc gacgagtacg gcgagtacaa gctgcagaac ccggcgaagt ttgccgtgtt
     1321 cgaggatcac ttgatctatg cgaccggcgt cgcctcgatg gcgaagttcg cagacaagat
     1381 cgagaagctg cgcgtgttgc agcgtgcgtt tgccgagaag accggcgtgc gcaattactc
     1441 ggcgaagaac ggcgtgtcgc cgggaatttg ccatcaggtg gcgcgcgagc agttcatcga
     1501 cccgggcgac ctcgtgcagg cgaccgactc gcatacgtgt atgggcggag cgtctggtgc
     1561 gcttgcgtgg ggcgtcggcg cgacggagta cgcggcgctg ctgtattggg gattcacgcc
     1621 gctcgcggtg cccgagtcga tccgcttcga gctgaccggg cggttgcctg cggggacgac
     1681 cgcgaaggac gtgatgctcc acatcctcgc gacgtatgcc aagcgtgagg acacgctcaa
     1741 tcgcgtcatg gagtttggcg gcgacgggct gtttgcactt tcgcccgacg agcgcgcaac
     1801 actagcgaac atggcgaccg agtgctcggc gcgcggcgcg gtgatggaag tcgacgagac
     1861 gatgcttgct tggatggcag agcgtcgccc cggcgtgaat gtcgatgact tgcgcgcgaa
     1921 ggctgtgttt ccggacaacg gcgcgcacta cgacggcggc gtgcatcgca tcgatctgtc
     1981 gacgatccga ccgatggtag cgacgccggg cgatccggat cgcggcatcg cgagtgatcc
     2041 gaagaatggc gcgctcgtga aagagatcgg gcgcgtaaag ctcgacatcg cgtacggcgg
     2101 ctcgtgcacg gccggcaagc gcgacgacgt cgacatgtac gcgcgcgtga tgcgcgaggc
     2161 ggaagccgct ggcaagcgcg tgcccgaggg cgtgaaattc ttcatccagt tcggttcgca
     2221 agaagtcgag gcgtacgcgc gcgcgcaggg ctacatcgac ttgttccagc gcacgggcgt
     2281 cgaggtaatc aagccgggct gcggcgcgtg catcggttgc ggcccgggtg tgtcggagcg
     2341 tggcgatcag gtcaccgtct cggcgatcaa tcgcaactac aagggccgtt cgggccccgg
     2401 caagctgtac ctggcttcgc cgctgacggt ggccgcctcc gccgtggctg gcgaaatcgt
     2461 cgaataccgc gacggtatgt tcggccccaa gaagtaggcg tttcttcgcg gttttgttag
     2521 ctttgatgcg cgatggccaa ggcgcgcaag aagccagcga aggccgtgcc gaaggcgaag
     2581 aagaagcctg cggccaagaa gcctgcgcct aagacgcgag ccgccgccaa gaagcccggc
     2641 gccaagaagg ccgccgcgaa gaagcccgcc gcgaagcgca ccaagaaggc ggccccgaag
     2701 aaggcggccc cgaagaaggc ggccccgaag cgtacccgtg tggtcgagcc caccgtcgag
     2761 gcgatcccgc aggaggtcat ctccgagccg tcgaccggcg gcacgctgcg caccctcacc
     2821 gcccgcgaac gtgcgatgcg gtcgctgccg atcgtcgaga tcctcgacga cccacatccg
     2881 atcggcgcgc ttcgtagatt cctcgactcg atccgcggcg aagcgacgca gcagcaagcg
     2941 cagatcgctc ttggctccgc gcagctcatg ctgctgccga tcgcgcggga gcatcgcggc
     3001 ggcgccgacg tgaaagagct catcgacctc gtgctccagc actggacggg gttgggcgat
     3061 cgccgcaagg gctaccacgc gcaggaattc ctacggaatg cactggccgc gatcggcgtc
     3121 gaccgcgagc gcatcgcgaa gctcgaggac ctcgttcccg cgaacgcgac gggtgagctc
     3181 ctgttcaatc tagcgggtgc gcatgctgtc gcgcgcgaca aggtcgccat gctgcgtgcg
     3241 gtcgagcgcg cgctcgaggc gggtgtgtca tcgtcgcgct ttcgccacga ctcggatttt
     3301 ctgccgtaca cgaaggatcc tgatctcgga cgactgctct cgcgcgccga agtgccgccg
     3361 atccccgtcg acgtcgagcc gtatctgcca gccgtgcgtg acgcgctcga catgctcgtc
     3421 gggacgctgc gcgaatacgg tgagtcgatc gagctgcgcc cgccggtccg gctcgacgcg
     3481 atcctcgatg ccgagcgcgc tcgcaaaatt tcgctgccga acgactaccg agcgctcttg
     3541 acgatcacga acggcatggc gctctgggag cacgccttct tcagcgtcgg cgactatcgc
     3601 gatcgcacac ccctcgccgc gcgcgcgcag gcgttcatcg acatgatggc cggcaacggg
     3661 cgcgagggaa tcgaggactg cgttcccctc gccaattggg ggcagccgaa cgactggctg
     3721 ctctacgacc gccgaggcac gctgcgcggc aacgaaccgg gctacgtgct catgctcaac
     3781 gccgacgagc acgcgctggg cgatttggcc gcggcgctcc accacctcga attcatcgca
     3841 cgcgagacgc tcggcacgaa ttgacgcgca attccgtcgt gaaaggccgt cttttgcgag
     3901 gaatgccgag caactaaacg ctcggcgggc gccactgcct ctaaatgcgc gacggtacgc
     3961 gggttgctct gccgtctgtt atggcgtccg agctgttacc actgatggag ctgcccattg
     4021 tgccgttgcg cacgatggtg ctgttccctc acgccgcgtt gccgatgact ctcaatcggc
     4081 caggagcgag tcaggcggtg tcggctgcag gcgaggaagg cctgctggca gtcgtgacgc
     4141 aggcaaatcc cgatgtggag atgcccagcg gtcgcgacct ttacgcgatc ggcaccgccg
     4201 ctgtcgtgag acagatggct cagccgggca ccgacacggt catggtcgtg ctggaagggc
     4261 tcgagcgcat cgcgatcgtc gaggtggtgc agacgcatcc ctatctgcgt gctcgcgtgc
     4321 gtcgactcac gagcgagaag ccgctgagcg actccgagta cgccgccgcg aagttgaacc
     4381 tgctcgaagc attctccgag atggtgcagc tctctcacgt gctgcccgac gagctgtcgg
     4441 tcgtcgccaa gagcctgtcc gacgatgccg cgctgacgga catgatcggt tccgcgctcg
     4501 tcgaggtccc gccgccaacg cggcaagaca tactcgcgac gctgaacgtc cgccgtcgca
     4561 tggacaagct gaacgaggtc gtcgcgcgcg agctgcagag cctgcggctg cgcgagaagc
     4621 tcaagacgga agttcagacc aagatcggcg agacgcagaa agagatcgtc cttcgcgaac
     4681 agctcaaggc gatcaagaag gagctcggcg acgagcatgg cgacgatcgc cacgtcacgc
     4741 agctcaagag ccgtctgcac cgcggcgagc tcaatgaaga agcgatgcgc gaagccgagc
     4801 gcgagctcga gcgactcgag gagatgtcag cggcatcgcc cgagcattcg atggtcagga
     4861 cgtacgtcga gtggctcgcc gagctgccgt gggtcgagac cactgcgaac gagatcgatc
     4921 tcaagcgcgc gcgccaagtc ctcgacgaag atcacttcga tctcgagaag gtcaaagagc
     4981 gcatcctcga gttcctcgcg gtccagcgcc tcaagcgttc gcagcgcggc ccgctgatct
     5041 gtctcgcggg tccgcccggt gtcggcaaga cgtcgatcgg ccgctcgatc gctcgcgcga
     5101 caggacgcaa gttcgtccgc atctgcctcg gcggcacgaa cgacgaagcc gagatccgcg
     5161 gccaccgtcg cacgtacatg ggctcgctgc ctggccagat catccgcggc ctgcgccgtg
     5221 ccggcagctg cgatccggtc ttcatgctcg acgagatcga caagatcggt cgcgatttcc
     5281 gcggcgaccc agcagccgcc ctcctcgagg tgctcgaccc cgagcagaac aacgcattcc
     5341 gcgatcacta cctcgacgtg ccgttcgatc tctcgaaggt gatgttcatc gcgaccgcca
     5401 acgttctcga caccatcccc gagccgctcc gcgatcgcat ggagatcatc gatctccccg
     5461 gctacgtcga cgaggacaag gtccagatcg cgaagcgcta cctcgtgccg aagcaggtcg
     5521 aagagaacgg gctcgtcctc ggcgaggaca tcgagttcac cgacgacgca ctgttcgagc
     5581 tcgtccacgg tcacacgcac gaagcgggcg tccgcaagct cgagcaagag atcgcgcatg
     5641 tcgctcgcaa gcgcgcccgc cagctcgccg agggcgacca aggcaagctg atcgtcaacc
     5701 gcggcgtcgt cacgggcttc ctcggccagc cgaagtacaa gatcgagaac caagtcgagg
     5761 aacgcacgcg ccgccccggt gttgctgtcg ctctcgcctg gacgtcgcaa ggcggcgagc
     5821 tcctttacgt cgaatcgagc aagctgccaa acggccgcgg cgacgtcacg ctcaccggcc
     5881 agatgggcga cgtgatgcag gagtctgcac gcactgccgt cagctggctc cgcggccacg
     5941 cccgccgcta cggcatcgcc gaggacgtct tcaagaagtc ggacatccac atccacgtgc
     6001 cggccggcgc cgtccccaaa gatggcccgt ccgccggcat cgtgatgatc acgtcactcg
     6061 tctccctcct gacaaaccgc ccgatccgtc cgttcacggc gatgacgggc gagatcacgc
     6121 tgtcaggccc gctcctgccg atcggcggcc tcaaggaaaa agtcctcgca gcccgtcgct
     6181 ccggcatcaa gcggatcatc attcctctcg aaaacgaacc aaacctgcac gaagaagttc
     6241 ccgctcacgt ccgtggtgat ctcgagatca tcttcgcatc cacgatggag caggcggtcg
     6301 atcttgcatt gaccccagac ggaggtgttc tcatggccca gcaaaagatt caggacgtga
     6361 tgacgaaagt cgtccactgc gtcggcgaaa acgcgacggt tcgcgaagtc gctcgcaaaa
     6421 tgcgcgacga atgcatcggc gatgttctcg tcaccgacgc ccaaggcaag ctcagcggaa
     6481 tcatcacgga ccgcgacgtc gtcgtacgcg cagtcgccga aggcggcgat ctcgactcgc
     6541 tccgcgccaa agacatctgc accgatcaca tcgtcacgct cgacccgagc tctggcgtcg
     6601 aggacgcggt gaagctgatg acggagaaag cgatccgccg catcccggtc gtcgagaacg
     6661 acgtgcccat cggcatcgtt acgatcggcg acctcgcagc ccagcgcgag ccggcctcag
     6721 cgctcggcca gatctcgact gcagcgccga ataattagcg gcgcttcttc gagatgacct
     6781 tgtgagcgat cacggtctcg atcacgaata cgaaaatcac gagcacgccg aacgttgcgc
     6841 ccgcgatcgg atcgttgttt ggaccgcgac caccaccgcc cgcgatcatc ttcatcgtga
     6901 agaacgtgaa gagcgcgccg atcacggccg cgacggcgat gctcttcagg cgagcacggg
     6961 tgttacgcag atccatcgat tccatgggag atagacacct ggttagcgcc ggagtttcag
     7021 cggatttacg ccggtcagaa tagcttcgcc ggtcgcggag atgcggattt ctgccaccgg
     7081 tgtcgcctcg atatccgaca tcgcgtagcg gtcggtgagc agctgaatcc gctggtttgc
     7141 ggagccgcgg aggccactcg cgaggcgctc ggtcgagacg tagcggccgt ctattaggac
     7201 gtccaggcgt gcgagaacgt ctggcccgtg gggcagcgcg cggatctcgt cgaccgtgta
     7261 gcccgagaac gcgagcgtcg tgaggccgag tgcgcgagcg gcgtcgagaa gcgcggccgc
     7321 cgcttcggct tgctggagtg gctcgccgcc ggagagggag aggccttcgc tgccggcgtc
     7381 gcgtgcgcgc gccatctcgg cgacgagctc gtcgatcgag acctcgcggc cgccgagtgc
     7441 gtcgtgcgtc gtcgggttga agcagcccgc gcagccgagc gtgcagcctt gcatccacac
     7501 gacgaagcga gagcccgggc catttgcgcg gctgcgagcc tcgctcgcgt gaatgcgaac
     7561 gcgcacgcgc tacttcttcg gcgcgggctt cttgtcgtcg ggcttcttgt cgtcggcctt
     7621 cttgtcgtcg cccttagcgg cggcggccgg cgctgcgacg acgcccttcg gcgcggccgg
     7681 tggcgggcga acgttgccgg tctggccgat gatgtcgatc tctttgagca gcaagtccgg
     7741 cgtgatgatg ctcgactcga tgccgcccga ggggacgaag ccgtcgtcgg tgccacccgt
     7801 gagacggagc tgcagctgcg gctcgctcgc ggcgaggaag ttatagaccg tcacttcctt
     7861 ggagacgccg atgacgtcct tccacgcgcg aatcgggacg tcggtgagct gcacgccgcg
     7921 cacgagctct tccttgccgt cggcggtgac tttgtacgcg agcagcgtcg gaggcggcag
     7981 ctcttggtcc gtcgtcttga ggagctggat gagctcgcgg cgcgtgaact cgggcgcgcc
     8041 cgtgaccgcg gcgtcgtcga agcggcgaac gacaaggccg tacttgaggc cttccgcgcg
     8101 cgcggccgag atgagcttgg acctgagcga tgcgcgcggc acgccgccct tcgccgtgac
     8161 gaacaggttc gtcgcgctgc cgtggaatgc accaccgtcg gcggcgcggc gcgcgtggcc
     8221 gttcgacgtc tgcttcttat ccgacggcgt gcgcgagagc agcagcgtct tcagcgtgcc
     8281 gtctttgatg acctcgacct tttgcgcggc gacgccttcg tcgtcgatct tgtagccgcc
     8341 gatcagcgcc ttgccgccgc cttgctgtgc ggtcggatcg tcgacgatcg agaggttgtt
     8401 cgcgaggacg cgcagcgtga ccttgtccgt gagcgcgccg ccgaacgact tcgcctcttg
     8461 tggcgagaga ccttccggca ccggcgtgcc accgaggtgc ggcgcgagcg cgaagcgaag
     8521 caggccgacc gcgccctcgc cttcgaacag caccgggccg gagtacttgt cgagcagcgg
     8581 cgccttcgcg agcgccgaga tcgacgcggc gaggttcttc gactccgtat cgagctcctt
     8641 gtcgctcggc aggtccttcg cggtgtggcc gtagcgcagg aagtagttgt tgagcagctg
     8701 gccgtcgggt gcctgaccct cgacctcgat cacgatgccg ctcgcccgac gcgtgtccgt
     8761 gacgctggtg ttctcggtcg tgatgtacca gcgacgctcg aggtagctcg tcagcgcgac
     8821 gcgcgaatcg cgcaggtccg gctgatcgcg aaacatcgcg gacaatttct tcgcgcgcgc
     8881 ttcgagatca tcgagtgtct cgagtggagc gacgagcact ggatcctcgc cgacgaccgg
     8941 cttctcgtcg gtccacgcgg ggacgtcggg atgtgaggtg ccaccggcgc ggcgcgcttc
     9001 gatcttcgcg cgcagctgga tcagcgcctc tttgtaggcc tggtcggtga cgagccacgc
     9061 ggcgcggcgt gcgatgcgag gcgtcgcttc gagcgggagg ttcgccgtcg cagtgccgtc
     9121 gagctctgcg ccgtcgggga cgacgaagtt accgttgtcg aactgcttgt tgccgacgcg
     9181 cacgcgcgcc tcgacgctga cgaagtgacg gttgcgcctc gacgtcgtct gaccgagcgt
     9241 cgccgagacg tcgttcacgt cgacctcggt gatcttgtac atgatgctgt acggcttcgg
     9301 cgcgcccgga atctcgagct tcgccatcgc gcgattcatc tcttcgtgaa tcgcatcgat
     9361 gatctcgtcg gtgacggcct tcgacggcgg gccgagctgc tcgggaatct tcttgccgtg
     9421 cgcgatgctc gggccgaagc tcaatccggc tgcgagtaga acgacgaggc gcttcatggc
     9481 gacaccttct gcttgatcga ggtcggtgca tcgaggagcg gcggccgatc gggcggaatg
     9541 aagcccttct cgacctcgag acgctcgatg agaagcgatg gcgccgacgc cgacacgggc
     9601 acccagccgg actctgcgcc gcacacgccg ttgaacgtct cgatctcgcg cgacgccgcg
     9661 cggatcgatt gcagcgcgac gagcggcgtg ccgctgatgt cgatgccgcg cacgagctcc
     9721 ttcttgccgt cggggaacag gcggtacgcc atcactggat tcaccttgaa tgcctgcggt
     9781 gcgaacgcgc tcgtgttcgt gaagccgccg ctgatgtccg tgaagatcat cccgtacggc
     9841 cgcttctgct tcttgatctc ctcgatcagc atcttctcga ggtcggcgta cggcaggctg
     9901 cgcgtggtct cgacgacgag gttgccctgg cgcgcgaccg gcggcagccc cggcgagtgc
     9961 cggccgtggc cgttcgagtg cgcgaagccc ggaatcggat tgcggcccat gagaaagcca
    10021 acgagcttgc ccttgtcgac gagcggcacg tgctgcgcct tcacgccctc gtcgtcgtag
    10081 cggaagaagc cgttgagctg gacgccgttg agcgtgacga tcgtcgggtc gtcatagacg
    10141 ctcagccact cgggcgcgat gtccttgccg acgtagctcg cgaacgtgcg gccactcgtc
    10201 gtgtccttct ggcgatgacc ctcgatgcga tggccgaaca cctcgtggaa gaacacgccc
    10261 gacgcgcggc cctggaggat cgccggaccg acgtagggat cggcgagcgg cgcgtcgtgc
    10321 agcgcgtcga ggtcattggt gacctccttg atcatcttgt cgacctcgcc gtcgctcggc
    10381 aggtccgcgg gcgtgcgacc aaaccgctgc tcgaggcgcg acaggttctc gccgtcgtcg
    10441 gccttcacgc cgacggagac cgacagctgc gcgttcgtcc acgacagctg gatctggctg
    10501 ccttcgctgt tcacgtagta cgcggtgttg agctggaaca gcacgccgca cgtg
//
LOCUS       DATLBZ010000631        15773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8784000, whole genome shotgun sequence.
ACCESSION   DATLBZ010000631 DATLBZ010000000
VERSION     DATLBZ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15773
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8784000"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VMZ53_30915"
     CDS             complement(<1..1158)
                     /locus_tag="VMZ53_30915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32969.1"
                     /translation="MESKNFALVLGVLAVTTQLARAETPLASRSADDQYSALHGHGMV
                     YAASAMDELGARVSQYGRFGYLEQCLGNGYEDNAPVQTLKWALCGDDVKALDMNAARE
                     HFSDYEFGERLKKVERWKELGAKIETEAKTDKGIAQVLELAAKAKSEWKDYLAANQAA
                     FDQFVKLHEALASGKTNEPAYQGCWEATQPAFAKAVKATKFAWEIESDPLTTRVEETL
                     TSTENYIAAANFSMCAFSVHASGEAMAAAVLQARGGTVRIGWRTILIAKALSPAFKPK
                     FADRKLNWTEHGMLFGLSDRGFLGDVGITSADAGTPKFAEITKVKKTGDDEVTLSFKA
                     DTRDACLQWVETNRISGFATNGDPMYQRVCKKRGKVDNTESDITISAKLAGG"
     gene            1272..2306
                     /locus_tag="VMZ53_30920"
     CDS             1272..2306
                     /locus_tag="VMZ53_30920"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HUS32970.1"
                     /translation="MRVVLAVLALSTGLAAGCAELGVVTDGSTVSFGKPNRGYLLDGV
                     KLPDSGAGFTTREVWSQRGNRYGTDELIALVKGVSKRMKKKVKDVRLVVADLSGTSGG
                     EKKEFHRSHQNGRDVDLLYYMRDAEGKPFEADAMHKFDRFGVALDGSGIQVDIPRTWL
                     LVKDLLTSNEAYVQYIFMYKPIVDQMLAYAMQQKEPDDLVARAAKACKQPGDSAPHND
                     HMHVRVYCAATDRQFGCVDIGPLELLAEREAEQHKVFEAIAASLPQEGAPAVPTTPTE
                     IANATLAAATPPAPATVAPLIAATIDPALPQLSATDVWTASASTAEATAAGTLSSSAA
                     ASFGTLFRAR"
     gene            complement(2307..3077)
                     /locus_tag="VMZ53_30925"
     CDS             complement(2307..3077)
                     /locus_tag="VMZ53_30925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32971.1"
                     /translation="MRRRKPLLLLLASTAVATLLAVAVHSSSVNADLENKIFTSRAHR
                     LRMVVPRGWRETDQPSYPGLLLWMLRSQPEGKMVLTAEPFTRQVYCSWPIECRSSPDS
                     LQSKFACAMRRKLEDQRLHVGPVQAGPKENEQAGVPSVWFELDDGHDFLRQAVAVRED
                     RLISLVLSARSAEARNAHVRYFEQALRTLRPLTPAELGEPLPSPQQVVTQLIDDAGIA
                     PTTDALAVTTDAAVAATTFESAPVQKAIDPVGPCSSTK"
     gene            3375..4106
                     /locus_tag="VMZ53_30930"
     CDS             3375..4106
                     /locus_tag="VMZ53_30930"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUS32972.1"
                     /translation="MRALSWTALWGIRLFTRDRSFETDAPSTTNVVPFRSHKRGARAL
                     VVSRRDDDATGGGAGAGGGGLVALTEEQLRAIETQYPEGLTAVQLVDAFTRNRVRFSE
                     ASFRKYVQQGLLPRSKRVGRKGKHKGSLGVYPAKTVRRINQVKRLMADGYTIEEIQGQ
                     VLLYTDLVEGVEENVTELVTRLQRDCERLEPASRKEVAKELTEVRKEGDRLVERLSAL
                     ATRVSAPRTDTLRLAGAAGGAEDLF"
     gene            4143..4763
                     /locus_tag="VMZ53_30935"
     CDS             4143..4763
                     /locus_tag="VMZ53_30935"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HUS32973.1"
                     /translation="MSADDRDDEDEAFELEAAEDVSADEDIEAASEVEASAETETNAE
                     GGELPEVSRKIRRRRRRTRPRSKTIAMKRLTREELRVGAAMYPPVDIPRPTTREECRG
                     EMRPCPWVACKHHLYLDINPETGSIKINFPDLEPWELKHTCALDVAERGGITLEEVGE
                     IMNLTRERIRQVEVRGLLKLKMGSPSPDELGAELLAQAALNAKDSN"
     gene            4777..5577
                     /locus_tag="VMZ53_30940"
     CDS             4777..5577
                     /locus_tag="VMZ53_30940"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HUS32974.1"
                     /translation="MHRLLALIAFAGCGRIGFDVASQPSDGRTDSSGDGMLIGHDEDG
                     DGVPDSIDVCPQVSDLQDDADGDGVGDFCDPKINTPGDRIAVFSSMAPGDQPFTLSTF
                     TTGAWTHSSDGFKLDGDLGADFFAGLTLPISAGDVRVDIGFDITAVGSGLQHQFALGT
                     TTRTPAYFAELNEQIGNYSGAYITYFDGSTYNQVDSRPLVLGIHPARVRFLLDERVNT
                     SVRANIQIDAEGYAPMVSDNTYQSSDLLQLNINNLQLEIRYVCVITSP"
     gene            5596..7194
                     /gene="purH"
                     /locus_tag="VMZ53_30945"
     CDS             5596..7194
                     /gene="purH"
                     /locus_tag="VMZ53_30945"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HUS32975.1"
                     /translation="MVRRALISVSDKRGVVELAKVLHELGVQILSTGGTASALASAGI
                     PVTQVSDYTGAPEILDGRVKTLHPKIHGGLLGRSTDAHQAEMKKHAIEPIDIVVVNLY
                     PFEATIAKSGVSFDEAIENIDIGGPSMLRSSAKNHERVAVVVDPADYDGVIADLRATK
                     EVSATRRLQLARKAFAHTAAYDTAIAAYLSSIDDSVASAREQEPKRAELPDTLGQQWR
                     RLYELRYGENPHQRAAFYADARSPLPTAASQRPTIATAEVLGGKQLSYNNILDLDAAL
                     GLCLEFAEPAAIVVKHNNPCGVATHSTLATAYQRARDADATSAFGGIVAVNREVDEAL
                     AKLLVETFLECVVAPGYSAAARDVLSSKKNLRLVAAHGAWTAPTNQLAWSLRTIAGGA
                     LVQTVDNGMVDLGDAKVASKRAPTEQERADLGFAWRVAKHVKSNAIVFAKDGVTLAIG
                     AGQMSRVDSVKICERKAGDKLQGSAVASDAFFPFRDGVDVLASAGAVAVVQPGGSVRD
                     EEVITAANEHGLAMLFTGMRHFRH"
     gene            complement(7208..7603)
                     /locus_tag="VMZ53_30950"
     CDS             complement(7208..7603)
                     /locus_tag="VMZ53_30950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32976.1"
                     /translation="MIHLLAISALLLSACGGGAKSTTTGPQQNASGETHGDTTGGAGQ
                     PSMDPTLPSWVPESCVAYHKVAIEAMGCTAIEQSKRDAIQQEYGAASQGWKAEPNADG
                     ARVEEIAAACVRSSNAVHADIAGKCAEAK"
     gene            complement(7717..8211)
                     /locus_tag="VMZ53_30955"
     CDS             complement(7717..8211)
                     /locus_tag="VMZ53_30955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32977.1"
                     /translation="MATAGLLSLSLGGCMASGQVHSSASVSAPDLVYIGPSVQVIADW
                     NEPVFYSSNYYWRYDGGVWYRSSNHLNGWMRIQTVPAAILQIERPTAYIHYRAQGSAN
                     ASAQPTQATPHDQRDAAHEARDERHEAKAEEKEERKEAKAEAKEERKDAKADHKDGKP
                     SKGH"
     gene            complement(8278..9288)
                     /locus_tag="VMZ53_30960"
     CDS             complement(8278..9288)
                     /locus_tag="VMZ53_30960"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HUS32978.1"
                     /translation="MSITLLVYVTIAVALLFDVINGFHDAANSIATVVSTRVLSPRLA
                     VVWATVFNFAALFVFHQGVANTIAKIIKIGGNDQAFVWVVICGLLGAIVWNLLTWWWG
                     LPSSSSHALIGGLTGAGLAYGGPAVLETANLYKTLYFIILAPVIGLLLGGLLMIAVIW
                     LFRRGRPRPVDRVFRIGQLFSAAAYSIGHGGNDAQKTIGVIWAVMVAGGMMPASATGA
                     PSWVIIASYSAMAFGTAIGGKRIIKTMGMGITSLKPIGGFCANLAGAVTLFGATYMHV
                     PVSTTHTITGAIVGVGSVTNVRRIRWGMATRIVWAWVFTIPLAGGVGALALVIARSLG
                     AW"
     gene            complement(9293..9904)
                     /locus_tag="VMZ53_30965"
     CDS             complement(9293..9904)
                     /locus_tag="VMZ53_30965"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HUS32979.1"
                     /translation="MPRETSFFDYFEQHAALTIVGTKEFLALVTPGANIPTSCQRITK
                     IENETDTITHRCVEALHKMFITPIDRDSIHRLITKMDDVMDYVEAASQCIELYKITEM
                     NDDTRDLADVLVRASQQVELALRGLRTLKQPQVALGICREIKRLENEADAIMRRSVAR
                     LFSDEGDLMKVIKWKEIYENLEMAADRCEDVANIIEGVILEHT"
     gene            10046..10285
                     /locus_tag="VMZ53_30970"
     CDS             10046..10285
                     /locus_tag="VMZ53_30970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32980.1"
                     /translation="MATRTTKALSIAVCAAIALQLASCGTILYPERRGTQGGRIDVGV
                     AVMDGLWCLVFLVPGVVAYIVDFSNGTIYEGGSRR"
     gene            10333..10659
                     /locus_tag="VMZ53_30975"
     CDS             10333..10659
                     /locus_tag="VMZ53_30975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32981.1"
                     /translation="MTTKPENSTDATRGSTLRARVEARMRELELALSTLSPTDRARPD
                     IEIALNGVRGLLTGDLDHIPYVVAAELSNWLEANKHVNERHAASDAASDAEDVATEPD
                     AAVDKP"
     gene            complement(10673..11716)
                     /locus_tag="VMZ53_30980"
     CDS             complement(10673..11716)
                     /locus_tag="VMZ53_30980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS32982.1"
                     /translation="MLRELLSANRSEIIDRCRARVAARLAPGVTEVEIQHVVPLFYDF
                     LVANLGGAAPVVRSTPGRNLLSGLTVAHVVHHYIDLCQTIRDVADERGVVFDARDQYD
                     LNACLDQAVAGVVTDFGRLSSTEDTDRATRDLGFLSHELRNLLCTAALALDTMRAGAI
                     GVESSTGAVLERCIGRMTKLVDRTLAAVRLNAGIGTHEHIAIGELMEEIEVSAMLEAK
                     ARGHRLVVEGTEPAAIVDGDRQILASILSNLVQNAFKYTEHGDVVIRSSATADRVRIE
                     VEDRCGGLPAGVAATLFEPYQQRGADRSGLGLGLAICLRGVSALRGTLAVRDIPGTGC
                     VFTVELPRVVNVP"
     gene            12004..12855
                     /locus_tag="VMZ53_30985"
     CDS             12004..12855
                     /locus_tag="VMZ53_30985"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUS32983.1"
                     /translation="MTTDVVSPTGEEHAKAEADNKAVADSKVTALVLPSPHDRSRPAY
                     QLEPKFDIPIIGIDLVFRFGRVAKTEKAYCAPVCDESELNAVDHLNAGFWNPTWSRAS
                     DIGLWSLRAGAVVLLTADEGIRNGLNDVVVVGEATFSASAVSLIMTLAAGRPRPYVFG
                     DPTQPDQLKAPLGTRNSPTASMSFLSGHTTESFAFVTSLYMTERRLHPHSKGPKIMLG
                     VGLTIATFVGVGRIMSGNHFITDVVGGAIVGSSVGVLVPALHRSPVSVVPIVSDTQAG
                     LGVEGAF"
     gene            12861..15098
                     /locus_tag="VMZ53_30990"
     CDS             12861..15098
                     /locus_tag="VMZ53_30990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32984.1"
                     /translation="MTKVLGTRAAAFTSAALIAYQVAGKAARDALFLTSFSVSSLPGV
                     MAIGAVLSLVSALWMSRLLTRYSPAVILPILFASSMCALIVEWGIGHVSPPAMAVAVF
                     LHSAVFGPLSISAFWSLINERFDPHSARQAVARIALGGTVGGVVGALIAWRVATLVAL
                     PTLVLLLAGMQGACLIGTFILRATARGHAVATNAPAAESQELVGVSAVTLLRREPYLR
                     NLAKLVALGAAMSAVLDFLFSRQAAVSFTKGSELLTFFSMFWLAVAVLSLLIQITLGQ
                     RALGKLGIAANVAILPIVVVTGGAVGLAVPGFATTSILRGAEAVQRNTLYRSAYELLY
                     TPLSEAHKRTIKAQIDIGCDRLGTIAASGLVIVFLAISASGAATYLLAMSVVLGLATL
                     TIVRKLHTGYVTALEHGLRDGATKLALPSLAREGTSNAQTVEDHEREALIQRVELLRP
                     RHDPSAQKPGDIVERPDGAGPAWNALRDPQALLDGRHLLAGDVAAITRELRELPLETT
                     TPLTGFLILLLAHKQLHAPALQALCRAAPVATGQLIDALLDPAMDFVVRRRIPRALAS
                     CASQRAADGLLLGLADERFEVRYACGRALIEISDANPGVVISRQKIVQAILIEVDRKA
                     PGIAAPQFEADDIEEASAAVDDVLARDRVDRTLEHVFTVLGLHLEREPMRMAYRALRH
                     PDVRHRGTALEYLQTILPKDLRDAVWPLVGALAPLPAARTAREILADLLRATVVQTKE
                     GGERD"
     gene            complement(15095..>15773)
                     /locus_tag="VMZ53_30995"
     CDS             complement(15095..>15773)
                     /locus_tag="VMZ53_30995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS32985.1"
                     /translation="ARREAGYVGQQGQFLATLGAELRPPLNAILGWIRMLREGSIRES
                     QRARALETIERSAVAQLELIEEMLDISRMTSRTLTLDLVNVDLRQIVEAVVEVHRPTA
                     LDKQVTLFAALDDDVAPMSADAERVRQIVHRLVKNALTSTPTEGIVTVSLRNTGSEVE
                     LAITDTSDDAQVGSQLFDGFAIVRHLVELHDGRIRIENAAPGRGRRFVIELPAAGRPP
                     RNDDEHR"
BASE COUNT         2744 a         5157 c         5057 g         2815 t
ORIGIN      
        1 cccgccggcg agcttcgccg agatcgtgat gtcgctctcc gtgttgtcga ccttgccgcg
       61 cttcttacaa acgcgctgat acatcgggtc gccgttcgtc gcgaagccgg agatgcggtt
      121 ggtctcgacc cactgcaggc aggcgtcgcg cgtgtccgcc ttgaacgaca gcgtgacttc
      181 atcgtcgccc gtcttcttca ccttcgtgat ctcggcgaac ttcggcgtgc ctgcatcggc
      241 ggacgtgatc ccgacgtcgc cgaggaaacc gcgatcagag aggccgaaca gcatgccgtg
      301 ctcggtccag ttgagcttgc ggtcggcgaa cttcggcttg aacgccggag agagcgcctt
      361 cgcgatcagg atcgtgcgcc agccgatgcg gaccgttccg ccgcgcgctt gcagcacggc
      421 cgccgccatc gcctcgcccg acgcgtgcac gctgaacgcg cacatcgaga agttcgccgc
      481 tgcgatgtag ttttcggtgc tggtgagcgt ctcctcgacg cgcgtcgtaa gcggatcgct
      541 ctcgatctcc caggcgaact tcgtggcctt gacggccttc gcgaacgcgg gctgcgtcgc
      601 ttcccagcaa ccttggtatg cgggctcgtt cgtcttgccc gacgcgagcg cctcgtgcag
      661 cttcacgaac tggtcgaacg ccgcctgatt cgcggcgagg tagtccttcc attcgctctt
      721 cgctttcgcg gcgagctcga ggacctgggc gatgccctta tctgtcttgg cttccgtctc
      781 gatcttcgcg ccgagctctt tccagcgttc gactttcttc aggcgctcgc cgaactcgta
      841 gtccgagaag tgctcgcgcg cggcgttcat gtcgagcgcc ttgacgtcgt cgccacacag
      901 cgcccacttg agcgtctgga ccggcgcgtt gtcctcgtag ccgttgccga ggcactgctc
      961 gaggtagccg aagcggccgt actgcgagac gcgagcgccg agctcgtcca tcgcggacgc
     1021 ggcgtacacc atcccgtggc cgtgcagagc gctgtactgg tcgtcggccg accgtgaggc
     1081 gagcggcgtc tctgcgcggg cgagctgggt ggtgactgcc aacacgccga ggaccaatgc
     1141 gaagttcttc gattccatga ctgtctcctc tttgccggcg ggcacagctt tgccgcgccg
     1201 cggcaagccg gcgtgacgcc ctaaaaacct ctgctcgttt tcggtgcgca catccctcat
     1261 agtgagaagg cgtgcgcgtc gttctcgctg tcctcgcgct atcgaccggc ctcgctgccg
     1321 gttgcgcaga acttggagtc gtcaccgacg gctcgacggt ttcctttgga aaaccgaacc
     1381 gtggctacct gttggacggc gtgaagctgc ccgacagcgg cgccggcttc acgacccgcg
     1441 aggtctggtc acagcgcggc aaccgctacg gcaccgacga gctgatcgcg ctcgtcaaag
     1501 gtgtgtcgaa gcggatgaag aagaaggtga aggacgtgcg gctcgtcgtc gccgacctct
     1561 cgggcaccag cggcggcgag aagaaggagt tccatcgctc gcaccaaaac ggtcgcgacg
     1621 tcgatctcct ctattacatg cgcgacgcgg aggggaagcc cttcgaagcg gacgcgatgc
     1681 acaagtttga tcgcttcggc gttgcgctcg acggcagcgg catccaggtc gacatcccgc
     1741 gcacgtggct gctcgtgaag gatctgctca cctcgaacga ggcgtacgtg cagtacatct
     1801 tcatgtacaa gccgatcgtc gaccagatgc tcgcgtacgc gatgcagcag aaggagcccg
     1861 acgacctcgt cgcccgcgcc gcaaaggcat gcaagcagcc gggcgattcg gcgcctcata
     1921 acgatcacat gcacgtccgc gtctactgcg cggcgacgga tcgccagttc ggctgcgtgg
     1981 acatcggccc gctggagctg ctcgccgaac gcgaagcgga gcagcacaaa gtcttcgaag
     2041 caatcgccgc gtcgctgccg caagagggcg cgcccgctgt cccgacgacg ccaacggaga
     2101 tcgcgaacgc gacgcttgcc gcagcaacgc cgcccgcgcc ggcgacggtg gctccgctga
     2161 tcgcggcgac gatcgatccg gcgctgcccc agctcagcgc gacggacgtc tggaccgcgt
     2221 ctgcgtcgac cgcggaagcg accgctgcgg gtacgctgtc gtcgagcgcc gctgcgtcgt
     2281 tcggcacgct gttccgcgca cgctaatcac ttcgtcgacg agcaggggcc gaccggatcg
     2341 atcgcctttt gcaccggcgc tgattcgaac gtcgttgcgg cgaccgctgc atcggtcgtg
     2401 acggcgaggg cgtcggttgt cggcgcgatg ccggcgtcgt cgatcagctg cgtgacgacc
     2461 tgctgcggag agggcagggg ctcgccgagc tcggccggcg tgagcgggcg caacgtgcgg
     2521 agcgcttgct cgaagtagcg aacgtgcgcg ttgcgcgctt cggccgagcg cgcgctgagg
     2581 acgaggctga taaggcggtc ttcgcgaact gcgacggctt ggcggaggaa gtcgtggccg
     2641 tcgtcgagct cgaaccagac tgacggaacg ccagcttgct cgttctcttt ggggccggct
     2701 tgcacagggc cgacgtgcag gcgctggtct tcgagcttgc gtcgcatcgc gcacgcgaac
     2761 ttgctctgga gcgagtccgg cgacgagcgg cactcgatcg gccaactgca gtagacctgg
     2821 cgcgtgaacg gctcggccgt cagcaccatt tttccctcgg gctgactgcg gagcatccag
     2881 agcagcaggc cggggtagct cggctgatcg gtttcgcgcc agccgcgagg caccaccatg
     2941 cgcagccggt gcgcgcgtga cgtgaagatc ttgttctcga ggtcggcatt caccgacgag
     3001 gaatggacgg cgaccgcgag aagcgtcgcg accgcggtgg acgccagcag cagcagtaag
     3061 ggctttcggc gccgcatctc ctgagtggag acgctagcgc gagcgccgat cgagcatcca
     3121 ctcgcgcgag gagatctcgc cgagccgagg ggtctagtgt cgcggtgaac ccgctacgca
     3181 atggctagcg tgcgacgccg attttagtgc tggctgcgtg taagacctga acagaactgc
     3241 agttaaaaag ttgattcgcc acgccagcat atcgttgacg gattatattc agacgaatat
     3301 cgtcacaata cctcgtcgaa cactggacct tccagcgtcg ggcagaggtg gagggcagca
     3361 gcgttgctgc gcccatgcgt gcgctgtcgt ggacggcgct ctgggggatc cgtttgttca
     3421 ctcgcgaccg aagcttcgag accgatgcac cgtcgacgac caacgtcgtc ccgttccggt
     3481 cgcacaagcg gggagctcga gcgctcgtcg tttcgcgacg cgatgacgac gcgacgggcg
     3541 gcggtgcagg tgcgggaggc ggcgggctcg ttgcgctgac ggaggaacag ctccgcgcga
     3601 tcgagacgca atacccggag ggtttgacgg cggtccagct agtggacgcg ttcacgcgca
     3661 atcgcgtgcg cttctccgaa gcgagctttc gcaaatacgt ccagcaggga cttctgccgc
     3721 gcagcaagcg cgtcggccgc aagggcaagc acaagggctc gctcggcgtt taccccgcga
     3781 agaccgttcg ccggatcaac caggtcaagc gcctgatggc cgacggctac acgatcgaag
     3841 aaatccaagg ccaggtcctg ctgtacacgg acctcgtcga gggcgttgag gagaacgtga
     3901 ccgagctcgt cacgcgcctt cagcgtgact gcgagcgact cgaaccggca agccggaaag
     3961 aagtcgcgaa ggaactgacc gaggtgcgca aagaaggcga ccggctcgtc gagcgactca
     4021 gtgcgctcgc gacgcgggtc tctgcacctc gtaccgacac gcttcgtctt gccggcgccg
     4081 cggggggcgc cgaggacctg ttctgaacgg acggcaaccc gcgccgacca ccaggagtaa
     4141 ctatgagcgc cgatgatcgt gacgatgaag atgaggcttt tgagctcgag gctgccgaag
     4201 acgtttcggc ggatgaggac atcgaggccg cgtcggaggt cgaggctagt gccgagaccg
     4261 agacgaacgc cgagggcgga gagctgcccg aggtaagtcg caagattcgt cggcgtcgcc
     4321 ggcgcacgcg gccgaggagc aagacgatcg cgatgaagcg gctcacgcgc gaagagctgc
     4381 gcgtcggcgc cgcgatgtat ccgcccgtcg acatcccgcg cccgacgacg cgcgaagagt
     4441 gccgcggtga gatgcggccg tgcccgtggg tcgcctgcaa gcaccacctc tatctcgaca
     4501 tcaacccgga gacggggtcg atcaagatca acttcccgga cctcgagccg tgggagctca
     4561 agcacacctg tgcgctcgac gtggcggagc gcggtggcat cacgctcgaa gaggtcggcg
     4621 agatcatgaa cctcacgcgc gagcgcattc gccaggtcga agtgcgtggc ctgctcaaat
     4681 tgaagatggg ctcaccgtct cccgacgagc tcggcgccga gctgctcgcg caagcggcgc
     4741 tcaacgccaa agacagcaac tgataccctc gagtagatgc atcggctgct cgccctgatc
     4801 gcgttcgcgg ggtgtgggcg catcgggttc gatgttgcga gccagccgag cgatggacgc
     4861 accgatagct ccggcgacgg aatgctgatc ggtcacgacg aagatggcga cggtgtccct
     4921 gactccatcg acgtttgtcc gcaggtttcc gatctgcagg acgacgccga cggcgacggc
     4981 gtgggcgact tctgcgatcc gaagatcaat acgcccggtg atcgaatcgc ggtgttctcg
     5041 tcgatggcgc cgggcgatca gccgttcacg ttgagcacat tcacgaccgg cgcgtggacg
     5101 cacagcagtg acgggttcaa gctcgatggc gatctcggcg ccgacttctt cgccggactg
     5161 acgctcccga tctctgccgg cgatgttcgc gtcgacatcg ggttcgacat caccgccgtc
     5221 ggttctggcc tgcagcacca attcgcactc ggcacgacca cgcggacgcc tgcgtacttt
     5281 gccgagctca acgagcagat cggcaactac agcggcgcgt acatcacgta tttcgacggg
     5341 tcgacctaca accaggtcga cagccggccg ctcgtccttg gcattcatcc agcgagggtc
     5401 cgctttctcc tcgacgaacg cgtcaacacg tcggtgcgcg cgaacatcca gatcgacgcc
     5461 gaagggtacg cgccgatggt cagcgacaat acctaccaga gctcggacct gcttcagctc
     5521 aacatcaaca acctccagct cgagatccga tacgtctgcg tgatcacctc gccgtgaacg
     5581 tggtacgcag cgggcatggt ccgccgcgca ttgatttcgg tttcggataa gcggggcgtc
     5641 gtcgagctgg cgaaggtcct ccacgagctg ggcgtccaga ttctctcgac gggcggcacc
     5701 gcttcggcgc tggcgtcggc ggggattccc gtgacgcagg tctccgacta cacgggcgcg
     5761 cccgagatcc tagacggacg cgtgaagacg ctgcacccga agatccacgg tggcctgctc
     5821 ggtcgctcga ccgacgcgca tcaagccgag atgaagaagc acgcgatcga gccgatcgac
     5881 attgtcgtcg tgaatctcta tccgttcgag gcgaccatcg cgaagtccgg cgtctcgttc
     5941 gacgaagcga tcgagaacat cgatatcggc ggcccgtcga tgctgcgctc gagcgcgaag
     6001 aatcacgagc gcgtcgcagt cgtcgtcgac ccggcggact acgacggcgt gatcgcggat
     6061 ctgcgtgcca cgaaggaagt ctccgcgacg cgtcgcctgc agctggcgcg caaagcgttc
     6121 gcgcacacgg ccgcgtatga caccgcgatc gccgcatacc tgtcgtcgat cgacgattcc
     6181 gtcgcgagcg cacgcgagca ggagccgaag cgcgcggagc tgccggacac cctcggccag
     6241 caatggcgcc gcctctacga gcttcgctac ggcgagaacc ctcaccagcg cgccgcgttc
     6301 tacgccgatg cacgctcgcc gctgccgacc gccgcatcgc agcgtccgac gatcgcgacg
     6361 gccgaagtcc tcggcggcaa gcagctctcg tacaacaaca tcctcgatct cgacgcagcg
     6421 ctcggcctct gcctcgaatt cgccgagccc gccgcgatcg tcgtcaagca caacaacccg
     6481 tgcggcgtcg ccacgcactc cactctggcg acggcgtacc aacgcgcacg cgacgctgat
     6541 gcaacgtcgg cattcggcgg catcgtcgca gtgaaccgcg aagtcgatga agcgctcgcg
     6601 aagctgctcg tcgagacctt cctcgaatgc gtcgtcgctc ctggctactc ggccgccgcg
     6661 cgtgacgtgc tctcgtcgaa gaagaacctg cggctcgtcg ccgcgcatgg cgcgtggacc
     6721 gcaccgacga atcagctcgc gtggtcgctc cgcacgatcg caggcggcgc cctcgtccag
     6781 accgtcgaca acggcatggt ggacctcggc gacgccaaag tcgcatcgaa gcgcgcgccg
     6841 acggagcaag agcgtgccga cctcggcttc gcatggcgcg tcgccaagca cgtcaagtcc
     6901 aacgcgatcg tgttcgcgaa agacggcgtc acgctcgcga tcggtgccgg ccagatgagc
     6961 cgcgtcgatt cggtcaagat ctgcgaacgc aaggccggcg acaagctaca aggctcggct
     7021 gtcgcatccg atgccttctt cccgttccgc gacggcgtcg atgtgctcgc atcggcagga
     7081 gcggtcgccg tcgttcagcc cggcggctcg gtgcgcgacg aagaagtgat cacggcggcg
     7141 aacgagcatg gcctcgcgat gttgttcacc ggcatgcgcc acttccgcca ctagaacaag
     7201 tcgccgccta cttcgcctcc gcgcatttgc ccgcgatgtc ggcgtggacg gcgttgctgc
     7261 tgcggacaca tgccgctgcg atctcctcga cgcgagcgcc gtctgcattc ggctcggctt
     7321 tccagccctg actcgcggcg ccatattcct gctggatcgc gtcgcgcttg ctttgttcga
     7381 tggctgtaca gcccatcgcc tcgatcgcga ccttgtgata cgcgacgcag ctttccggca
     7441 cccacgaagg caacgtcgga tccatgctcg gctgaccagc gccgcccgtc gtgtcgccat
     7501 gcgtctcgcc actcgcgttt tgctgcggcc ccgtagttgt cgacttcgca ccgccaccgc
     7561 aagcggagag gagcagagcg ctgatagcta gaagatgaat catgtgccgg tgcgcaaagc
     7621 acggatgatg ccaatcgcag tcgtcggcag cttttgcgac cgcgggccaa ttcgcccagc
     7681 cgtgggcgcc tggtgcccgc aacgagccga ggcagctcag tgacccttgg acggcttgcc
     7741 gtccttgtga tcggccttcg cgtccttgcg ctcttccttc gcttcggcct tcgcttcctt
     7801 gcgctcttcc ttctcttcgg ccttcgcttc gtgacgttcg tcgcgtgcct cgtgagcggc
     7861 gtcgcgttgg tcatgtggag tcgcctgcgt gggttgtgcg ctcgcgttcg cgctcccctg
     7921 cgcacgatag tggatgtacg cggtcggccg ctcgatctgc aggatcgcag cggggaccgt
     7981 ctggatgcgc atccaaccgt tcagatgatt cgacgagcgg taccaaacgc cgccgtcgta
     8041 acgccagtag tagttgctcg agtagaagac cggctcgttc cagtcggcga tgacctgaac
     8101 gctcggacca atgtagacaa ggtcaggagc gctgaccgac gcgctcgagt gcacctggcc
     8161 agacgccata cagccgccga gcgacagaga cagcaagcca gcggtagcca attgaagcaa
     8221 tggatttctc atgtgccgag cacgtcgagc accatccatg ccgcatatag atgtcgccta
     8281 ccaggcgccg agggagcgcg cgatgacgag cgcgagcgcg ccaactccgc ctgcgagtgg
     8341 aatcgtgaag acccaggccc acacgatcct cgttgccatg ccccagcgga tgcggcgtac
     8401 gttcgtgacc gagcccacgc ccacgatcgc gcccgtgatc gtgtgcgtcg tcgacacggg
     8461 cacgtgcatg tacgtcgcgc cgaacaacgt gaccgcaccg gcgaggtttg cgcagaagcc
     8521 gccgatcggc ttgagcgatg tgatgcccat gcccatcgtc ttgatgatgc gcttgccacc
     8581 gatcgccgtt ccaaacgcca tcgcgctgta cgaggcgatg atgacccagc tcggggcacc
     8641 ggtcgccgac gccggcatca tcccgcctgc gaccatcacg gcccagatga cgccgatcgt
     8701 tttctgcgcg tcgttgccgc cgtggccgat ggagtacgcg gccgccgaga acaactgccc
     8761 tatccggaag acgcgatcca cggggcgagg tcgtcctctg cggaacagcc agatcactgc
     8821 gatcatgagt agaccgccga gcaagagtcc gatcaccggg gcgaggatga tgaagtagag
     8881 cgtcttgtag aggttcgcgg tctcgagcac tgcaggaccg ccataggcga gccctgcacc
     8941 ggtcaatccg ccgatcagtg cgtgcgagct cgacgacggt agtccccacc accaggtcaa
     9001 taagttccaa acgatcgcgc cgagcagacc gcagatgaca acccacacaa acgcctggtc
     9061 gttgcctccg atcttgatga tcttcgcgat cgtgtttgca actccttggt gaaacacgaa
     9121 cagcgcggcg aagttgaaca cggtggccca cacgacagcg agccgcggcg acagcacgcg
     9181 cgtcgacacg accgtcgcga tcgagttcgc ggcatcgtgg aacccgttga tgacgtcgaa
     9241 caacagcgcg accgcaatcg tgacgtagac gagaagcgtg atgctcattg atctacgtgt
     9301 gttcgaggat cacgccctcg atgatgttcg cgacatcttc acaacgatca gctgccatct
     9361 cgaggttctc gtagatttcc ttccacttga tcaccttcat cagatcgcct tcgtcgctaa
     9421 acagacgcgc gaccgaacgg cgcatgatcg cgtcggcctc gttttcgagc cgcttgattt
     9481 cccggcagat accgagcgct acctgtggct gcttgagcgt ccgcaagccg cgaagtgcca
     9541 gctcgacctg ctgcgacgct cgcaccagca catcggcgag atcgcgggtg tcatcgttca
     9601 tctcggtgat tttgtagagc tcgatgcatt gcgatgccgc ttcgacgtag tccatcacgt
     9661 cgtccatctt cgtgatcagg cgatggatcg agtctcgatc gatcggcgtg atgaacatct
     9721 tgtgaagcgc ctcgacgcag cgatgcgtga tcgtgtcggt ctcgttctcg atcttcgtga
     9781 tgcgttggca gctggtcggg atgttcgcgc cgggcgtgac gagggcgaga aactctttcg
     9841 ttcccacgat cgtcagcgcg gcatgctgct cgaagtaatc gaagaagctg gtctcacgag
     9901 gcaatagacg gccgaacata cgcacctccg cgatagagcc cgacatgacc tgtcgaacgc
     9961 gcgccgctcc atctgcaagt ccgcggccga acatcgggcg aagcgagcag cgagcgttgg
    10021 tatacgagtt gctgcgcgca accgcatggc aaccaggacg accaaagcgc tttcgatcgc
    10081 tgtctgcgct gcgatcgcgc tgcagctcgc gagctgcgga acgattctct atcccgagcg
    10141 ccgcggtacc cagggcggtc gcatcgacgt gggcgtcgcc gtgatggacg ggctttggtg
    10201 cctcgtgttc ctcgtgccag gtgtcgtcgc gtacatcgta gacttcagca acggcaccat
    10261 ctacgaaggt ggtagccgcc gctgatcgag atgggacgta cgtggctcgg ttcgtgctcg
    10321 ggggagcgcg cgatgaccac gaaacctgaa aacagcacgg atgcgacgcg cggatcgacg
    10381 ctgcgcgctc gagtcgaggc acgcatgcgc gagctcgagc tagcgctgtc gacgctttcc
    10441 cccaccgacc gcgcgcgacc cgacatcgag atcgctctca acggtgttcg cgggttgttg
    10501 accggcgacc tcgaccacat tccctatgtc gtcgctgccg agctgagcaa ctggctcgaa
    10561 gcaaacaagc acgtcaacga acgtcacgcg gcctcggatg cagcctcgga tgccgaggat
    10621 gtcgcgaccg agccggacgc cgccgtcgac aaaccctgaa ggtcgcgcgt cgttacggaa
    10681 cgttcaccac gcgcggaagc tcgacggtga acacgcaacc ggtcccagga atgtcgcgca
    10741 cggcgagtgt gccgcggagc gctgacacgc cgcgaaggca aatcgccagg ccaagtccca
    10801 gaccgctgcg gtcggcgccg cgctgttggt atggctcgaa tagagtcgct gcaacacccg
    10861 cggggagccc accgcagcga tcctcgacct cgatgcggac tcgatcggcg gtggcggagc
    10921 tgcgaatcac gacgtcaccg tgctcggtgt acttgaacgc gttctgcacg aggttcgaga
    10981 ggatggacgc caggatctgg cggtcaccat cgacgatcgc ggccggctcc gttccctcga
    11041 cgacgagtcg gtggccgcga gccttggcct ccaacatcgc cgagacctcg atctcttcca
    11101 tgagctcgcc aatcgcgatg tgctcgtggg ttccgatgcc tgcgttcagt cgcaccgccg
    11161 ccagcgtgcg atcgacgagc ttcgtcatcc ggccaatgca tcgttcgagc accgcgccgg
    11221 tgctgctttc gacgccgatc gcgcctgcgc gcatcgtatc gagggccagc gcggcggtgc
    11281 acaagagatt gcgtagctcg tgcgacaaga agccaagatc tcgcgtcgcc cggtcggtgt
    11341 cttcggtcga gctcaggcga ccgaaatcag tcacgacccc agccaccgct tggtcgagac
    11401 acgcgttcaa gtcgtattga tcgcgagcat cgaacaccac cccacgctcg tcggccacgt
    11461 cacgaatcgt ctgacagaga tcgatgtagt gatggacgac atgcgcgacc gtcagtccgc
    11521 tcagcaggtt gcggccgggc gtcgaacgga cgacgggtgc cgccccgccg aggttcgcga
    11581 ccaagaagtc gtagaacagc ggcaccacgt gctggatctc gacctcggta acaccgggcg
    11641 ccagccgagc cgcgaccctt gcgcggcagc gatcgatgat ctccgagcga ttggcactga
    11701 ggagctcgcg cagcatggct ggaggggtct tgggatttta ccgccggctc ggacaacaaa
    11761 aacgtcccac tcgagcattt ctgtcccgtt gtgatcgccg gagggcgcaa gggaacttgg
    11821 tccgccacgt gctggtggcc gtggtgaatg cgaatgacct atctgataca aggcctggcg
    11881 ctcgcggcgc tggcgacctc tgtcgcatac gcggacaccg gtagcgctgc gccaaccccg
    11941 gctcctacac cggcaccgac acccgctgca gatgccgccg ccgcagggga tggatcagcc
    12001 caagtgacga cggatgtcgt ctcgccaacc ggcgaggagc acgcgaaggc ggaggccgac
    12061 aacaaggcgg tcgccgactc gaaggtgacc gcacttgtcc tgccgagccc gcacgatcgc
    12121 agccggccgg cataccagct cgaaccgaaa ttcgacatcc cgatcatcgg tatcgacctc
    12181 gtattcagat tcgggcgcgt cgctaagacg gagaaagcgt attgcgcacc cgtgtgtgac
    12241 gagagcgagc tcaacgctgt cgaccacctg aacgcgggct tctggaaccc gacctggtcg
    12301 agggcgtccg acatcggtct ctggagcctc cgggccggag ctgtggtgtt gctgacggcg
    12361 gacgagggca tacgcaacgg tctcaacgac gtggtggtgg tcggggaggc gacgttcagt
    12421 gcatcagccg tatcgttgat catgacgctg gccgcgggcc ggcctcgacc ctacgtcttc
    12481 ggtgatccga cacaaccgga tcagctcaag gcgccgctcg ggacccgtaa tagtcccacc
    12541 gcctcgatgt cgtttctcag cggccacacc accgagtcgt ttgcattcgt gacgtcgctc
    12601 tatatgacag agcgcaggtt gcatccgcac tcgaagggac cgaagatcat gcttggcgtc
    12661 gggctcacga ttgccacgtt cgtcggtgtc gggcgcatca tgtcgggcaa tcacttcatc
    12721 acagacgtcg ttggtggcgc gatcgtcggg tcgtccgtcg gcgtgctggt accggcgcta
    12781 caccgctcgc ccgtgtcggt cgttccgatc gttagcgaca cgcaggcggg cctcggggtc
    12841 gagggcgcct tctaacgacg gtgacaaagg ttctcgggac gcgcgcggcc gcgttcacgt
    12901 ccgccgcatt gatcgcatat caagttgcgg gcaaggcggc gcgcgacgcg ctcttcctga
    12961 cgagcttctc cgtgagctcg ctgccaggag tcatggccat cggcgccgtg ctctctctcg
    13021 tttcggcgct gtggatgtcg cggttgctca cgcgctactc gcccgccgtc atcctgccaa
    13081 tcctcttcgc ctcgagcatg tgcgcgctca tcgtcgagtg gggaatcggt cacgtatctc
    13141 cgccggcgat ggcagtcgcg gtgtttttac acagcgcagt gttcgggcct ctgtccatct
    13201 ccgcattctg gtcgctgatc aacgagcgct tcgatcccca cagcgcccgt caggcggttg
    13261 ctcgcatcgc gctcggcgga acggtcggtg gagtcgtcgg tgccctcatc gcgtggcgcg
    13321 tcgcgacgct ggtcgcgctg cccacgttgg ttctcttgct cgcgggcatg cagggtgcct
    13381 gtctgatcgg gacgttcatc ctacgcgcaa ccgcgcgggg gcatgccgtc gcaacgaatg
    13441 cgcccgcggc ggagtcacaa gagctcgttg gcgtctcggc cgtgaccttg ctccgccgcg
    13501 agccatatct gcggaacctc gcaaagctcg tcgcactcgg cgccgcgatg tccgcggtgc
    13561 tcgattttct tttcagccgg caagcggccg tgagcttcac gaagggctcg gagctgctca
    13621 cgttcttctc gatgttctgg ctcgcggttg ccgtgctctc gctgctgatc cagatcacgc
    13681 tcggtcagcg cgcgctcggc aagctgggca ttgccgcgaa cgtcgcgatc ctgcccatcg
    13741 tggtcgtgac cggcggtgcc gtcggcctcg ccgtgcctgg cttcgcgacg acgtcgatcc
    13801 tgcgcggggc ggaagcggtg cagcgcaaca ccttgtatcg ctccgcgtac gagctcctct
    13861 acacgccact gtccgaggca cacaagcgaa cgatcaaagc tcagatcgac atcggctgtg
    13921 atcgcctcgg cacgatcgcc gcgtccggac tcgtcatcgt gttcctcgcg atctcggcgt
    13981 cgggcgctgc gacgtacctg ctcgcgatgt cggtggtgct cgggctggcg acgctgacga
    14041 tcgtccgcaa gctgcacacg ggttacgtca cggcgctcga gcatggactg cgcgatggcg
    14101 cgacgaagct cgcgttgcca tcgttggcgc gcgagggcac gtcgaacgca caaaccgtcg
    14161 aggatcacga gcgcgaagca ttgatccagc gcgtcgagct cttgcgccct cgccatgatc
    14221 cgtcggcgca aaagccggga gacatcgtcg agcgccccga tggtgcgggc cccgcgtgga
    14281 acgcgcttcg cgacccgcag gcattgctcg acggacgcca tcttctggcg ggcgatgtcg
    14341 ccgcgatcac ccgcgagctc cgcgagttgc cgctcgaaac gacgacgcca ctgacggggt
    14401 tcttgatctt actcctcgcg cacaagcaac ttcatgcccc cgcgctccag gcgctgtgtc
    14461 gcgccgcgcc ggtcgctact gggcagctca tcgatgcgct cctcgatcct gcgatggact
    14521 tcgtcgtgcg gcgtcggatc ccacgggctc ttgctagctg cgcatcacag cgcgcggcgg
    14581 atggcctcct cctaggcctc gccgacgagc gattcgaagt tcgttatgca tgcggccgcg
    14641 cgctgatcga aatctccgat gcgaacccag gcgtcgtgat ctcgcgacaa aagatcgtgc
    14701 aagccatcct catcgaggtc gatcgtaagg ctcccggaat cgcggcgccg cagttcgagg
    14761 ccgatgacat cgaagaggcg agtgctgcag tcgacgacgt gctcgcgcgc gatcgcgtcg
    14821 atcggacact cgagcacgtg ttcacggtcc ttggcctgca cctggagcgt gagccgatgc
    14881 gaatggcata tcgcgccttg cgtcacccgg acgtgcgtca ccgaggtacc gcgctcgagt
    14941 atctgcaaac gattctgccg aaagatctgc gggacgccgt gtggccgctc gtcggcgcac
    15001 ttgctccatt acccgcagca cgcacggcac gcgagatcct cgcggatctg ttgcgggcta
    15061 ccgtcgttca gacgaaggag ggcggcgagc gcgactagcg atgttcgtcg tcgttgcgcg
    15121 gcggtcgtcc cgcggccgga agctcgatca cgaaccggcg gcctcgcccc ggcgccgcat
    15181 tctcgatcct gatccgaccg tcatgaagct cgactagatg acgaacgatc gcgaacccgt
    15241 cgaacaattg cgaaccgacc tgcgcgtcgt cactggtgtc tgtgatcgcc agctcgacct
    15301 cggatccggt attgcggagc gagacagtga cgatgccttc ggtcggcgtc gacgtcagcg
    15361 cattctttac gaggcggtgc acgatctgcc ggacgcgctc tgcatccgca gacatcggcg
    15421 caacgtcatc atcgagtgcg gcgaacagtg tcacctgctt gtcgagcgcc gtgggccggt
    15481 ggacctcgac gaccgcttcg acgatttggc gtaggtcgac gttgacgagg tcgagcgtga
    15541 gcgtccggct cgtcatccga ctgatgtcga gcatctcttc gatgagctcg agctgcgcga
    15601 ccgcgctgcg ttcgatggtc tcgagtgccc gggcgcgttg tgactcgcgg atcgacccct
    15661 cacgcaacat gcggatccat ccgaggatcg cattgagcgg aggacgtagc tcggcgccga
    15721 gcgtcgcgag gaactgcccc tgttggccga cgtagcctgc ctcccgccgt gcg
//
LOCUS       DATLBZ010000632        31350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7924578, whole genome shotgun sequence.
ACCESSION   DATLBZ010000632 DATLBZ010000000
VERSION     DATLBZ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 31350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31350
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7924578"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(722..1744)
                     /locus_tag="VMZ53_31000"
     CDS             complement(722..1744)
                     /locus_tag="VMZ53_31000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32986.1"
                     /translation="MRRLVAFVLAASACGGTQSTRLTRGGDNDEGAGQLARASLKLSM
                     DSGETTDDDGFAEDVIDDEYASRRYGRYGDPYGGDPYGGDPYGGDPYGGTSYANWRMP
                     QWNYNTPNRQPDYQITVGLPGAIEGSVSWNGALPAKLASPCGGGAIDNPTLRISSDKR
                     VRGVIVYIENIEVGRATPYYTRPASVGGVLAKHGCTLAPAAQIVAPLPGSVEIHGDAT
                     KAKIKITPPKAKVQSQEIEEAGLVVTEIEDGVTRVDGDDGKLGAAWVIGLATPYFAIT
                     DDTGRYRIDELAAGTYDVTFWQPPIASIARDGTWSYSAPIVAKRKVTVGSKTAQLSVT
                     LSPPSR"
     gene            complement(1810..3384)
                     /locus_tag="VMZ53_31005"
     CDS             complement(1810..3384)
                     /locus_tag="VMZ53_31005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32987.1"
                     /translation="MVSAFLHVVVVAVWWFAHRPKEPEKEYVDIEMAPAPPKAEALPA
                     EVAKPPESSPAATTPTPPGPEPSAGAHDDEGGIDAGIDAPIDAPPKKKKKRPDAALVA
                     EGDDAGADDGDASQQIALADAGAGDDGAGAIASIEDATVAEGSAVAVEHPPVHPNEPG
                     GSGVGSGVVAATEQGSGSGAPGMDNQPAVDGAPTSAGTAANLLAYFPAGHQITALVRF
                     DRLRGTEWAEPAEKLFQPMPDYRALFGARDAKIADKLDSLVISTPRPRDVVATTLVGH
                     SSLSRADLRTFLSNPDTPIKWSTTNGGLYGTRSGKLFQNDRRVLLSPWKNWFVLAQPE
                     DVAGLTTATKGSLDTAEAKAKLPAWLAGIRTIEQESEEPASTAVATPAKPTTTKRGPA
                     LVLTLVGPGKRYKFPDVVGLGITSAPSPVRLSLAMELVKQGWLVRGNIVFATEADATE
                     FAQTAKDIQQRINDSRFLSGILKKQHAYNAVAGLSVAQSGARVSYATSLSIADARAVM
                     AVMAATLTDYFEGQQP"
     gene            3525..3923
                     /locus_tag="VMZ53_31010"
     CDS             3525..3923
                     /locus_tag="VMZ53_31010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32988.1"
                     /translation="MKLTKEQVAAVVTEASNKMSDPNYASVMVGGFVQSQPPVSNFIS
                     AHERELGGAEGVVSVIFHCALVAECYKRNGGKVRTLSYEDLDAAARGEPLVRLEKAQL
                     PLHEFIKANVENEEAQKLIAMIALAIDSMS"
     gene            3946..4464
                     /locus_tag="VMZ53_31015"
     CDS             3946..4464
                     /locus_tag="VMZ53_31015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUS32989.1"
                     /translation="MANPTAIFDTSLGQFTAEIYLDKMPITANNFIKLAKEGFYNGLH
                     FHRVIKDFMIQFGCEYSKDPNSPRCGTGNSPFGNVQDEHPPNAKLSNEPGTLSMANTG
                     RPNSGGAQFFINTVHNAYLDWFSPGPSKHPVFGKIVSGMDVVTKIGTTPTAAGDRPKT
                     PIKMNSVTIQGA"
     gene            complement(4487..6511)
                     /locus_tag="VMZ53_31020"
     CDS             complement(4487..6511)
                     /locus_tag="VMZ53_31020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HUS32990.1"
                     /translation="MTSALEPVPDPIRTTTIDNDPTQEWLVTNGLGGYASGTLLGVPT
                     RRYHGLLVAALPNPAGRVMMLASLPAQLRLADGKRIDVGWVPPVYSRGMPMNLVEFRL
                     ELGLPVWRYEGAGITLEKRIVVSYGTNTMMVVYKLLDGGPVRLELRPGCQFRGHDDSV
                     STKVPDHYPLRAEGSRIEIIAPSPIPALKLHMQGTHTSFVLEPSCVPDLAYSTEESRG
                     YESRGQLYSPGRFRAQLDPGTQVSMFASTESWDTLLAMPSDDLLAAELERRRRLLDAA
                     VPPARTGPAAELVLAADQFVIRPAGRREDHVRARASGDEAWTVIAGYHWFTDWGRDTM
                     ISLEGLTLLTGRVQEAGSILRMFAQHIRDGLVPNMFPEGANEGVYHTADATMWFFHAL
                     DRYLHASGDRRTLTKLLPTLRDVIDAHLRGTRFNIRVDPDDGLLHQGAPGYQLTWMDA
                     KAGDWVVTPRRGKAVEINALWYNALRLMSDWCARTGDPDGAKQMHDLAERTYASFNRR
                     FWNASAGYLFDVVDGEHGNDAACRPNQLFSISLPNPVLDPARWKSVLDTCERELVTPA
                     GLRSLSPHHPDYKRTYRGDLLTRDAAYHQGTVWSWLIGPYIDAYLRVHGEDTAGARGK
                     LEGLIAHLGAACIGTISEVFDAEAPFLPGGCAAQAWGVAELLRCLVRTSR"
     gene            complement(6508..8400)
                     /gene="treZ"
                     /locus_tag="VMZ53_31025"
     CDS             complement(6508..8400)
                     /gene="treZ"
                     /locus_tag="VMZ53_31025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HUS32991.1"
                     /translation="MSRRLPIGAEIAEGGVHFRVWAPAWKQVTLVLEGPVRREIALEP
                     EAHGYHARFVDGIGAGERYRFRLGDQLHADPASRAQPDGPFGPSLVIDPRDAAWTATQ
                     WQGITRPETQVVYEMHIGTFTPEGTWNAAAAHLPFLKDVGITTIEMMPVNEFAGRHGW
                     GYDGVNLFAPHHHYGTPADLRAFIDRAHVLGLGVILDVVYNHFGPAGNSHFVYAPEMK
                     RTDLPGEWGDPLNYRSRGMRELVISNARYWIDEFHFDGLRIDATQAIFDDTKPHILAD
                     IVRVTRAAARSKRIWIVGENEPQDTRMVAPIDDGGMGLDAIWNDDFEHSTRVALTGVT
                     DGYFHDYAGTPQELVAAIEHSFLYQGQIFAWQRNTRGTPTRGLSASRFVHFLENHDQV
                     ANTGFGDRMITLTDPSSYRALTALLLLGPQIPMLFQGQETGSTKPWRFFCDHDSELCK
                     LVKKGRAEFLQQFARLATPEAQAALPDPGAAATFSECILDPSERSFDRSCVQLHRDLL
                     RIRRETPAFIDGDLRGAVLTDRAFCIRWWHHTGHRLLLVNLGSTLRQAVLPQPLLAPP
                     PDHGWRLEWSSEDPTYGGHGTPEVFTSKRLAIPARCAVLLAPDRDAYLRVDPPPPSGE
                     KELPDL"
     gene            8541..9548
                     /locus_tag="VMZ53_31030"
     CDS             8541..9548
                     /locus_tag="VMZ53_31030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32992.1"
                     /translation="MRKRLLALLLVAACGGKTKHVDTVPLPPDPKVAEEEKKPVEEKP
                     ADPPAPTPQPLDVPIATPKPTVKLVNAGKGKKAALKIAPKAGAKQTIEVALDFIGGQD
                     GPPEAGGKQDQTAPTVILAADIETKEVGTDGQSKFQLTISGVEAKDAPGSKVPAAQFK
                     SELQALIGSSIAGSVGADGSTTGLTLHMDAVSDQKQLGALAYLKTALLPLWPVLPAEA
                     IGPGAKWTVTSTEKIADQVEVTKTVNYELVANKSGAWTIKGTTKLTGADQELIADPEK
                     PDMKAKVGSIGGGGTIEATVTDGALVSPVKQKMTSDFTITAQTVNIKFHLEQSNAVTT
                     K"
     gene            9561..11252
                     /locus_tag="VMZ53_31035"
     CDS             9561..11252
                     /locus_tag="VMZ53_31035"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUS32993.1"
                     /translation="MRWGISLVVALAACVAACRPVRIKPVQPKVDEPKLDIVAPWLDP
                     SDFWPRGTPEKRRPLSDALADPGTPPIVIRNATILTATGQRISNGTIVVDKGVIAYVG
                     DGTDATTPDGATIIDGQGKFVTPGIIDAHSHLGVYAAPNAQANDDGNEMSSPVTAQAR
                     AEYGYWPQDPQIARAVAGGVTTSLILPGSANLVGGRGFTVQMKPARTAREAAFPGAPS
                     TVKMACGENPKRVYANKGGPQTRMAEYSAFRSAFLEAQAYAAKQNAYVKLRQQWSEKR
                     LRAAEIESKTKDKKRKIPGEVAPEAPPVDLKLETLAGVLRGDVLVQIHCYRADEMANM
                     INVAHEMGFHIRSFHHALEAYKIRDVLAAEGIAINTWADWWGFKMEAYDGIPENAALM
                     TEAGGRATIHSDSAIGTQRLNQEAAKAMASGRRAGVNIDEDTALKWITANPAWVLGID
                     SVTGTLEIGKRADVVMWNGTPFSAYARADIVVIVGQVAYDRSKGMRASDFELGHGVLD
                     VGMPTSPLPAPTAKTLMPKGPAPRTPAPQTAPAPQTAPAPQAPPPPPASPPGGVR"
     gene            11249..12697
                     /locus_tag="VMZ53_31040"
     CDS             11249..12697
                     /locus_tag="VMZ53_31040"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUS32994.1"
                     /translation="MRAQDTMTVARAVEGRSVAMTAARAVGGRSVAMTVAQAVERREH
                     AITAARAIEPRALAITARVLAQLAVFVAVVVVATSASAAPIAIVGAKVYVTPDNVVEN
                     ATVVIDNGKITAVGAGGATPAGATVIDGKGKVVTAGLIEAVSAVGLVGVDLEATSVDG
                     RFGPTDPVHDDPVHASYDARDGFAPHDVTVAVARTGGITLVVAAPGGALVAGQSAAYT
                     LDGSAEAVKAQTAMHIELGSNAASGGSRGKTLELLREILDDARAYGRDKGAYERNAKR
                     HLIADRLDLEALQPVLAGRMPVIIAADSEADIRAVLRIAKDFKLRVAITGGAEAWHVT
                     KELAAAKVPVFVDPMQNLPGSLAASDVHDDAAKVLFSERVTVGISTLGAAGMARTLRQ
                     LAGNAVGHGLPWARALAAITTVPAQLYGLTGRGTVEKGSIADVVVWSGDPLEVSTAAE
                     TVIIGGVVQSNETHQTKLLKRYRTLPAKMTGN"
     gene            complement(12719..13807)
                     /locus_tag="VMZ53_31045"
     CDS             complement(12719..13807)
                     /locus_tag="VMZ53_31045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUS32995.1"
                     /translation="MSAAAINSLFDKLLERGGSDLHLTPGYPPMLRVRGQLVAEGARE
                     LTSGDIEAMLMPLLSSEQRNTFDTTGDLDFAHAHGTKARFRANYLRKLTGTGAVFRAI
                     PTIIKTLDELGVPPGIRKLADLRAGLVLVTGPTGSGKSTTLAAMIDHINRTRPGHILT
                     IEDPVEFVHQPRKCLITHKEVGEHVPSFLDAIRSAGRENADVILVGELRGAETMKAAL
                     QLASFGILIYATVHTNSAAATIDRYVNAFPADQQAQIRGLLADSLAGVVAQQLLPKSD
                     GQGRVAVHEILLGSLALASVIREAKTQQIASLIQQGMSEGMQAMDAALEKLVRAGTIK
                     AEAAWEKALDKESFAKTFPQLKAAAEVR"
     gene            complement(13804..15903)
                     /locus_tag="VMZ53_31050"
     CDS             complement(13804..15903)
                     /locus_tag="VMZ53_31050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUS32996.1"
                     /translation="MTNLTARPVSLDQLTNMLRGSPVAALIPEADSTAAPVDVQIGAR
                     RVRVQIGRRGDEVLVRLTRPDGVATTMPIQRIAEPAARTPDQAPTSAHAQSSSAARAK
                     SPTASRAKSPTGAGAKSPTGARAKSPTGARAKSPTATRATSPSSSRAKSPTSSRAKSP
                     TSARAKTPTTGRAKRPTAARAKSPTKRPSIDIGMPSPASLVGASKAIPAGASMFSTAS
                     SPAPASASTLGPATAPTRDLSPSGLFDALDLDGDIELGAPVGAALGSANAPALGTQRG
                     AARQPASPGHGIPTAIDVDAPTHSSSAGIVIPSIRERVEIDAPTHTSSPSIALPPVSA
                     SDATHPVTVPHAQSSAVPKATGELAALVEAARARGASDLHIAAARVTSARIGGQLLPL
                     DGTQPLSAAEVEGLLLPLLDREMRTTLNRVGYVDFALEAPDGGRLRTNLCKQQQGLKG
                     TFRLAMPGPLPLDKLGLPQELAKVVSHHQGLVVIAGPSGHGKTTTLAALVDLVNSTKP
                     HHILTIEDPVEIVHPRKMAVVSQREVGRHTTSFARGLKASLREDPDVIVIGELRDRES
                     VEIALTAAETGHLVLATMSTPSAAKTIDRLIDMFPPEEHSQVRSSIAGALRAIVAQRL
                     LPKKGGGGVVPAVELVTGVLPLAVMIREDKMYQLPNLMQRGRAFGMIRLEESIAELVR
                     TGRIEDDSKTQLLWKVK"
     gene            complement(16026..18194)
                     /locus_tag="VMZ53_31055"
     CDS             complement(16026..18194)
                     /locus_tag="VMZ53_31055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32997.1"
                     /translation="MQHRWQFFRAGAVDQVSLRDGFDLLALPELDQKLWVALAMPTKG
                     IDVDPATLELLDANKDGRIRVQDILAAVKDIKQWFKNPSELLTSSDEVDLAAFSDAKV
                     VAAAKRMLADLGKKDAETITIEDTIAVTKAFADTVLNGDGIIIPASADAGPVRQAIED
                     AIACVGSLNDRSGKPGLDKALADQFFGDVDKQAAWLAAVGPAKTMGEATDAAGDALRA
                     VKAKIEEYFTLCKLAAFDPETVPATIKLTSLDPASLRALPLAKVDTASRLPLRVGVNP
                     AWIDALGTFTDKTVTPILGACDALTPTSYKAVCDKLAAYDAWYAGKPDTKVGALSPDR
                     IKALAAPEIRAKVADLIAADSALTTEYEQITSVVKAVRFQRDFGRIVRNFVNFSDFYS
                     KKDGVFQAGTLYLDARALHLCIPVTDAGKHGALAASSAACLLYCDITREGVTKQIAAA
                     LTNGDSDNVFVGRNGIFYDRDGNDWDATITKIVTNPISVREAFWAPYKKLVKVVEDNV
                     TKRAAAADDAASARMTAAGTQIAHVDKAATKDAVAPAADPKAAPKKIDLGTVAAIGVA
                     IGGIGTLVGALLATMFGLGKWLPLGILALLLMISGPSMLLAWLKVRRRNLGPILDANG
                     WAINSRTRINVSFGAAMTELAAIPKHSKRVLSDPFADKTPPWRLYLASATLLVLAGTW
                     YVGRLDTYLPDAVRSTKVLGKYAPAYKAPAATPAATPPPS"
     gene            complement(18215..20569)
                     /locus_tag="VMZ53_31060"
     CDS             complement(18215..20569)
                     /locus_tag="VMZ53_31060"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HUS32998.1"
                     /translation="MGSWTVRVLVVLALVGCGDKAPSSMPDGNTPDAAVDAPDAAVTS
                     SSCGNGVVEGTEQCDFGVDPAAGAGCEANCTFSCSGNSCDDGDLCNGAELCASVTVDG
                     HDGMACTAGTAAAPGTSCGSGKICINNACATGQCGDSFTTAPEECDDANQLLGDGCEN
                     NCKFSCVSTDATRNCTPTDSCKGQGTCNNTTHTCSAGTPLADNTACGTGGYCKQGTCT
                     QPVCGNSLVELGEACDHGSANGTANDGCKSNCQWVCTLATDCGTAPACKKEQCTVQHT
                     CQAVADSTLNGMSCGGGGLVCSSGSCVSPAAVCGNGVVEAGEQCDFGSGMNTAGSGCE
                     PSCQFSCTTSPNSCSDGNACNGTETCGGVTVNGKAGQKCSSGTALTDGSSCGTLRICR
                     SQACTNSTCGDGYIDLLNGESCEPGNTATCDSQCRTILCGDGIRAGNEQCDDGNLTNL
                     DGCNGTCKFEQVHRVNQLMIPFTGPYAFCTKNAMGESMVGSSVRTNITDAFRSGVTDG
                     SIAVIFAFLGLDDLSGTSDASVSVAWIKGPPVTSTKTYNGNTDLDWWYTTNAADIDSA
                     RVPKTQMPGAIAAKVLTAGPAEVVVSASFSGVICYFDMVDAKVRVGINGASAPTVSTD
                     DMTPGHIAAEHLNPALTSFESMGTVTRDTNGNITKLSGEVCGNTTAESLDGSAVPAVM
                     LGSTPCAENYSASHTLLDVFVNGCKTFGLFTQVKGTQPDASRDGAKYTFQSDATRKII
                     GCTRCVGTTCVSSGSQGGPTLASCKYNAAYTSVFMITTDRVIAK"
     gene            complement(20637..24071)
                     /locus_tag="VMZ53_31065"
     CDS             complement(20637..24071)
                     /locus_tag="VMZ53_31065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS32999.1"
                     /translation="MKGAARLATLAAGLAFAACAESPLVPCGDLVCPPGNVCTAGGCA
                     APADVEACVGLSDGDPCRAATGGSGICQGGACHTGLCGNARIDVGEVCDGDVGIDAVL
                     GQTCSGDCTQIYECGNAVVDPAEQCDDGNKNPADGCDACGQTAWKAAAIVGSRIAATA
                     IPLAAPNAIAVDGQGRVFIADTSNHRVLRIEADGSVETLAGTGAAGYVGDGGAATSAQ
                     LAAPSGIAVDGIGRVYVADTGNHVVRLIDLDGTITTVAGVGVGGYDIQDEGRPAILAM
                     LKAPHGLAVDGLGRLYIADTENQRVRRVDIDGRINTVAGNGVPGYSGDAGPAVDASLQ
                     TPFAVAIDTQDRMLVADTENNAVRRVDAAGVITTVTSGLSFPTGIAVDAQGTVYVADT
                     GNQGIAKVLTNGTVASVAGTGTQGFAGDGGPAAAATLANPLSVAVDPSGRVLIADTSN
                     QRIRRIDTTVSPATIDTIAGTGSFGLGGEGGQANSALLTNLYGVAVDAQGQIIIADAF
                     NDRIRRVALDGTITTIAGAGTFGFSGDGGPATQAQLGHPFYVSLDPQGRVVFIDTFNF
                     CIRRIETNGTITRIAGTGVSGYNGENLSATSSKISRAYGTAYDASGRLIIADTYGQRI
                     RRVELNGNMVTIAGTGTLGYNGDNIAATSAQLGYPYDVAVDNQGRVVFSDTSNNRVRR
                     IEGNGTIVTIAGTGTAGFNGDGIAATSAQIDSPYGIKIDANGLVVFADSNNMRIRRIQ
                     ANGTITTIAGGAAGPNGDAGPATSAQLGTPLGIAIDPQNRLLVADTGNRRVRRVELDG
                     TIHNVAGKVDPDSTGPVASARLSDPQQLAISSDMTLVAGGASGTLEIIRNSRVDAVAG
                     RYPQNSPTSTLARFRTQAFGSVGGVAYDEANHRIFVSETSSNRIHVITQVNPADPSTW
                     TIAALANTAGVAGFADGSAGTARFRGPTGLYYDAGLLYIADTDNHAIRVLNTMAATVS
                     TLVNTTHRLGFAGDGGLAAGALLYRPTALTRCANGNMFIADTGNNRVRRLENVGSGSE
                     TISTVLGDGVFASSGEGSPARTFPVAAPRGVTCDSFGNVFVSSTTTVRMLAAGDNGVV
                     DGAGNVFTIYGAPPRVSFPSSVTTCLTAVASTGSTTLQISDRCTGLLIELVRGAAQ"
     gene            24155..27046
                     /locus_tag="VMZ53_31070"
     CDS             24155..27046
                     /locus_tag="VMZ53_31070"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS33000.1"
                     /translation="MSGPKTTDGNELPTVNMPLGPARKTSESTEVLGNQVFARLSVSG
                     HTRVDKRPPLDFSKNKQIGRFEMIRELARGGMGQVFLARDTKLGRKVAIKFLLHDDPD
                     FVQRFLVEARATARCTHENIITIFEVGEHEGLPYMVLEYLEGKTLSQVLETRPSQQQF
                     TELMIPVLRALERAHADGIVHRDLKPSNIFVTDRGQVKVLDFGVARLIDRAGEMVANV
                     SAHELRLDEHSSSVTRAGSNSLVGTMPYMSPEQWGAGEVDHLSDLWAIGIMFWRALTD
                     VHPAGTMHPEKLRARLLDLVTPLPGLRDQNPGVPEELAHIVDKCLAKNREHRYQNASD
                     VLHDLQAFAAPRDVPRGTETCPYRGLAAFGEDDAKFFFGRSNEIRTALAQLDSWPLLA
                     VIGPSGVGKSSFVHAGLVPAIRTSGSWNVRVLRPGRAPLLRLAAVLEEALATGEQSND
                     LLGQLKDAPGLFGKMLRTSAARRNQRVLVVVDQLEELFTLCDSDEHRKIFLAALLAAA
                     DDPSSPVRVVLSMRADFLDRLAGHKHFLSELSRGLFFLTAPDHENLRETLIRPAELAG
                     YSFEDERIVEDMMLAASSRGALPLLSFAATRLWDARDRTKKQLTSKAYEQMGGVGGAF
                     ARHADQVAASVPPQSQILLRAIMSRLVTPDGTRAVVDKEELLSISDNRQDIEQIIDTL
                     VRARLIHVQTDNETTTVEIVHEMLITEWPTLKRWLEDSHVLRSFLHELRQAARQWDSH
                     GRSNDHVWRGAPAHEALSLAKRHVLELSAVESAYLRAVRTQAARARRTRVLVFASVLA
                     VLVVILFGGAWFTVQLAKANEEARDKEANWHHAAQEAQAAKADLQQKLDIINEKERLR
                     LAAEASAKQAHEEVLKADEGLKLSQEELEKANAELRRALVEAQNEKHRAQNAAELAKK
                     SSDEAKQAKALAEAAAAKEKARADKLEQESKSIYNKDLRRKPLGGGSGQ"
     gene            27043..27999
                     /locus_tag="VMZ53_31075"
     CDS             27043..27999
                     /locus_tag="VMZ53_31075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33001.1"
                     /translation="MRALVLLSLLIAGVAHADSVAPWEQGIPDAQQAKANALFAEANS
                     LFAEKAHPAALEKYKAAVAVWDHPMIRFNMAVTLIRLDRMLEAADELEKALRFGAAPF
                     TPELYEQAVDYQALVMKQLGTIEVRCDQPGTHVLLDGKPFFDAPGTKQVRTTSGEHSI
                     VGEKRGFMTVARRIVVPGGKTAKETLQLLPVESAIVVEYKQPRWMPWVATATSAAIVG
                     SGVAFVVAGNHEMDRFTDAFIRVCPSGCEADLSMHRDLADQQDSAMLKTKIGVSLLVG
                     GGVAMVGSIVWGVTNRRTRRIGPRVEVSPTASGASASASWTF"
     gene            complement(28000..28542)
                     /locus_tag="VMZ53_31080"
     CDS             complement(28000..28542)
                     /locus_tag="VMZ53_31080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33002.1"
                     /translation="MERAQREMERAQRQMARDVSRMGHGGNCDGDDDDDGDGDDDGAF
                     NVDIQFDEDQMQIQLQQQLDQLNDKLQRKLEKLQKKLQKMHFKLGKKGFRGAWVMPPM
                     PPMPPMPPMPPMPVMPVIVVPPTPPTPPTPPTPPTPPHWHQHDLAAPPPVPPTRAPVR
                     VQRLPDGGMKIDYDLDVDIE"
     gene            28528..29046
                     /locus_tag="VMZ53_31085"
     CDS             28528..29046
                     /locus_tag="VMZ53_31085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33003.1"
                     /translation="MRALHLALRLLLLSLRAFLLSLRTLLLALGDHLSAIGFELGDLL
                     GALSFTLRDLLCALRHLLCLLGGSFRGGLGRTFRGFLHLVFVVLIVVVVVIAIVVIVV
                     VVAIVVVIIVGLGDAVVVIIVVACDRARGVGDQQSSDQGRDEQHERAHESSWHPKSRE
                     TPRRWTRKFSPT"
     gene            complement(29090..29485)
                     /locus_tag="VMZ53_31090"
     CDS             complement(29090..29485)
                     /locus_tag="VMZ53_31090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33004.1"
                     /translation="MKRRWTVLFYCALGSLIGMSWLAKSLDISLGSFWGVSLMGMIAA
                     SGVAFFAMWRNVPDKWPAAIVLTCAGPMILDVLRTIRELPMILRFFGPSIALVVIGAV
                     ATAGVSIAILVMQPPRPAAPPVAPARVVD"
     gene            complement(29597..30574)
                     /locus_tag="VMZ53_31095"
     CDS             complement(29597..30574)
                     /locus_tag="VMZ53_31095"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HUS33005.1"
                     /translation="MRDVAQRLRRLLFIVPYVAKHPEGVAVEQLAKMIGAERDELLAD
                     LDLLSQVGPPDGDPGEYLLVSVEEGRVFVDLAHRLTRPLRLTPAEGCSLLLGIRTLRE
                     SGIAPFDAAMQSAERKLLSALGRDANEAQSLATSTVVAEPDAAVASHLRTLVTAARKR
                     ERVEIDYASASRHQSEHRPVDPYGIVHHAGEWYFVGHCHNRKDVRTFRIDRIAAIKTT
                     GERFDRPADFDLEAYRRERLYVPSADAVTVRVQLDPLAVTRIGASWPVGEVTMQDDGS
                     AELLVDCEGFEWVTGWVLGLGRHAWIVGPDEARKAMHDRVSRLRGELRA"
     gene            complement(30567..>31350)
                     /locus_tag="VMZ53_31100"
     CDS             complement(30567..>31350)
                     /locus_tag="VMZ53_31100"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HUS33006.1"
                     /translation="KLAFDLPELPDTPTEFPRPAHNAPTVLVHFPERQNRLASSAVAR
                     PELGEIYATLESATRLRKRVTLTYQAATNGLVSRRDLDPYAMVYREGAWLVVGWCHLR
                     HEIRSFRVDRIHEAVQAPKPKSPDFERPADFDVKAYAQRSPWTFTSEPAEEVQLVLTA
                     EAAEVANEDFGPTAVKRHDGDRTLITFDCTNPEFAVSRILASKGAIQVTRGDKLRARI
                     AGELDAIAERYETFADLDAARVAANGHGPIDVIEGSGRDLDA"
BASE COUNT         5182 a        10140 c        10763 g         5265 t
ORIGIN      
        1 cggcacatcg catcgagcgc actcgcgtcg aaccgccgcg cccacgccgg tacatagcat
       61 cgaaagcacg cgcgccgaaa cgccgcggcg acgtagcgga acgatcggac cacgccgaac
      121 ggccgcgccc acgtcgcatc gaacgcgcgc cgagacgccg cggcgacata gcgcaacgat
      181 tggacgcacg ccgaaccgcc gcgcccacgt ggcacatcgc aacggcgtcc gccgaaccgc
      241 cgcgcccacg tgggcacatc gcgacggagg ggcgcgtgag cacgtcgcgt cgatcaacgc
      301 acaccgcacc gccgcgccca cgtgggcaca tcgcgacgga cgcgcgtcca accgcggcgc
      361 gtaggtgcgc acatcgcaac ttcgaacgac gctctccgcc gcgctgacgt gagcacatcg
      421 cggcgatcca acgtacgccg aaccgccgcg cccacgtggg cacatcgcaa cggcgtccgc
      481 cgaaccgccg cgcccacgtc ggcacatcgc atcgaacgcg cgccgagacg ccgcggcgac
      541 atagcgcaac gatcggaccc cgccgaaccg ccgcgcccac gtcggcacat cgcggcggac
      601 ccgcgtccaa ccgcggcgct tacgtgcgcg catcgcagcc ttcgaacgac gctctaccgc
      661 cgcactgacg tgagcacatc gcggcgcacc cgcctacgcc cacgtgggca cgccgaacgt
      721 gtcagcgcga cggcggcgag agcgtcaccg acagctgcgc ggtcttcgag ccgacggtga
      781 ccttgcgctt ggcgacaatc ggcgcggagt acgaccacgt tccatcgcgc gcgatcgagg
      841 cgatcggcgg ctgccagaac gtgacgtcgt aggttcctgc ggccagctcg tcgatgcggt
      901 agcgaccggt gtcgtcggtg atcgcgaagt acggcgtggc gaggccgatg acccatgctg
      961 cgccgagctt gccgtcgtcg ccgtcgacgc gcgtgacccc gtcttcgatc tcggtcacca
     1021 cgaggccggc ttcttcgatc tcttgcgatt ggacctttgc cttgggcggc gtgatcttga
     1081 tcttggcttt tgtcgcgtcg ccgtggatct cgacggagcc cggcagcggc gcgacgattt
     1141 gggccgcggg ggcgagcgtg cagccgtgtt tcgcgagaac gccgccgacg ctggcggggc
     1201 gcgtgtagta cggcgttgcg cggccgactt cgatgttctc gatgtagacg atcacgccgc
     1261 gcacgcgctt gtcggaggag atgcggagcg tggggttgtc gatcgcgccg ccgccgcatg
     1321 gcgatgcgag cttggccggc agcgcgccgt tccacgagac ggagccttcg atcgcgccgg
     1381 ggaggccgac tgtgatctgg tagtcgggct ggcggttcgg cgtgttgtag ttccattgcg
     1441 gcatccgcca gttcgcgtag ctcgtgccgc cgtagggatc gccgccgtag gggtcgccgc
     1501 cgtaggggtc gccgccgtac ggatcgccgt aacggccgta gcgccgcgat gcgtactcgt
     1561 cgtcgatgac gtcttccgcg aagccgtcgt cgtcggtggt ctcgccgctg tccatcgaca
     1621 gcttgagcga tgcgcgtgcg agctgaccgg cgccttcgtc gttgtcgccg ccgcgcgtga
     1681 ggcgcgtgga ctgcgtgcca ccacatgcgg atgcggcgag cacgaacgcg acgagacggc
     1741 gcatcctgca agcatgccgc gtcgacggga aaggttcaaa cgccacgggc gtctgacacc
     1801 gaaggcgggc tacggttgct ggccttcgaa ataatcggtg agtgtcgcgg ccatgaccgc
     1861 catgacggcg cgggcgtcgg cgatcgagag cgatgtcgcg taggagaccc gcgcaccact
     1921 ttgggcgacc gataggccgg ctacggcgtt gtaggcgtgc tgcttcttga ggatgccgga
     1981 cagaaagcgg ctgtcgttga tgcgctgctg gatgtctttg gcggtctgcg cgaactcggt
     2041 ggcatcggct tcggtcgcga acacgatgtt gccgcggacg agccagcctt gtttgacgag
     2101 ctccatcgcg agcgagaggc ggacgggcga tggggcggag gtgatgccga gaccgacgac
     2161 gtcggggaac ttgtagcgct tgccgggacc gacgagcgtg agcacgagcg cgggacctcg
     2221 cttggtggtc gtcggcttgg cgggtgtcgc gaccgcggtc gacgccggct cttcgctctc
     2281 ttgctcgatg gttcgaatgc cggcgagcca ggcgggcagc ttggccttcg cttcggccgt
     2341 gtcgaggctg ccttttgttg cggtcgtgag gcccgcaacg tcttcgggct gtgcgagcac
     2401 gaaccagttc ttccacggcg agaggaggac gcgacgatcg ttttggaaca gcttgccgct
     2461 gcgcgtgccg tagagaccgc cgttcgtcgt cgaccatttg atcggcgtgt cgggattgga
     2521 caggaaggtg cggaggtcgg cgcgcgagag gctcgagtgt ccgacgagcg tcgtggcgac
     2581 gacgtcgcgc ggccgcggtg tcgagatgac gagcgagtcg agcttgtcgg cgatcttggc
     2641 gtcgcgcgcg ccgaacaatg cgcggtagtc gggcatcggt tggaacagct tctcggcggg
     2701 ctcggcccac tcggtgccgc gcaatcgatc gaagcgaacg agcgccgtga tctgatggcc
     2761 ggcgggaaag taggcgagca ggtttgctgc ggtgcctgcg ctcgtcggtg cgccgtcgac
     2821 agcgggctgg ttgtccatgc ccggagcgcc ggagccgctg ccttgttcgg tcgcagcgac
     2881 gacgcccgag ccgacgcccg atccgcccgg ctcgtttgga tgcaccggcg gatgctcgac
     2941 cgcgacagcg ctgccttccg cgacggttgc gtcctcgatg ctcgcgatcg cgcctgcacc
     3001 atcgtcaccg gcgcctgcat cggcgagtgc gatctgctgc gaggcatcac cgtcgtcggc
     3061 gcctgcgtca tcgccttcgg caacgagtgc cgcgtccggc cgcttcttct tcttcttcgg
     3121 cggcgcatcg atcggcgcgt cgatgcctgc gtcgatcccg ccctcgtcgt cgtgtgcgcc
     3181 agcgctcggc tccgggccgg gcggcgtcgg cgtggtggca gcgggcgagg actcgggcgg
     3241 cttcgcgacc tcggctggca gtgcctcggc cttcggtggc gcgggcgcca tctcgatgtc
     3301 gacgtactct ttctccggct ccttcggccg atgcgcgaac caccacacgg cgacgacgac
     3361 gacatgcaga aacgccgaca ccatgatcgc gcgtgcgatc caggatgcgc ccgtagaaaa
     3421 cggttcgacc agaggcctat gcgttgtgcc ggtcacggtt ctcgacgggc gacctcctgc
     3481 gcagtagcat gcctcggacc ggcgatcacg cccatcgaga gcccatgaaa ctcacgaaag
     3541 aacaagtcgc cgcggtggtc accgaggcgt ccaataagat gtcggatccc aactacgcga
     3601 gcgtgatggt gggtgggttc gtgcagagcc agccgccggt ctcgaacttc atcagcgcgc
     3661 acgagcgcga gctgggcggc gccgagggtg tggtcagcgt catctttcac tgcgctctcg
     3721 tcgccgagtg ttacaagcgc aacggcggca aggtccggac actttcgtac gaagatctcg
     3781 atgccgcagc gcgtggtgag ccgctggtcc gcctcgagaa ggcgcaactc ccgctgcacg
     3841 agttcatcaa ggcgaatgtc gagaacgagg aagctcagaa gctcatcgcg atgatcgcgc
     3901 tcgcgatcga ctccatgtcg tagcgagtcg tgttaagcga tcggcatggc gaacccgacc
     3961 gcaatcttcg atacctcgct cggccagttc acggccgaga tctatctcga caagatgccg
     4021 atcacggcga acaacttcat caagctcgcg aaggaaggct tctacaacgg ccttcacttc
     4081 caccgcgtga tcaaagactt catgatccag ttcgggtgcg agtactcgaa ggatccgaac
     4141 agcccgcgct gcggcaccgg taactcgccg ttcggcaacg tgcaggacga gcacccgccg
     4201 aacgcgaagc tctcgaacga gccgggcacg ctctcgatgg cgaacacggg ccgtccgaac
     4261 tcgggtggcg cgcagttctt catcaacacc gttcacaacg cgtatctcga ctggttctcg
     4321 ccgggcccgt cgaagcaccc ggtgttcggc aagatcgtct cgggcatgga cgtcgtgacc
     4381 aagatcggca cgacgccgac ggccgcgggc gatcgcccga agacgccgat caagatgaac
     4441 tccgtcacga tccagggcgc gtagtcagcg acgacgcagc cgcgtctcag cgacttgtcc
     4501 ggacgaggca gcgcagaagc tctgccacgc cccacgcttg ggcggcgcaa cctccgggga
     4561 ggaacggcgc ttcggcgtcg aatacctcgc tgatcgtgcc gatgcacgcg gcaccgaggt
     4621 gcgcgatgag gccttcgagc tttccgcgtg cgcccgcggt gtcttcgccg tgcacgcgca
     4681 ggtacgcgtc gatgtacggg ccgatgagcc acgaccagac ggtgccttga tgataggcgg
     4741 cgtcgcgcgt cagcaaatcg ccgcgatacg tccgcttgta atcggggtga tgcggcgaca
     4801 gtgagcgcag gccggcgggc gtgacgagct cgcgctcgca ggtgtcgagc actgatttcc
     4861 aacgcgccgg atcgagcacg ggattcggca acgagatcga gaacagctga ttcggccgac
     4921 acgcggcgtc gttgccgtgc tcaccgtcga cgacgtcgaa caggtagccc gcgctcgcgt
     4981 tccagaaccg gcgattgaac gatgcgtacg tccgctcggc gagatcgtgc atctgcttcg
     5041 cgccgtccgg atcgccagtg cgcgcgcacc agtcggacat gaggcgcagc gcgttgtacc
     5101 agagcgcatt gatctcgacg gccttgcctc ggcgcggtgt gacgacccag tcgcccgctt
     5161 tcgcgtccat ccacgtgagc tggtagccgg gcgcgccttg gtggagcagg ccgtcgtcgg
     5221 gatcgacgcg aatgttgaag cgcgtgccgc gaaggtgcgc gtcgatgaca tcgcgcagcg
     5281 tcggcagcag cttcgtcagc gttcggcgat cgccgctcgc gtggagatag cgatcgagcg
     5341 cgtggaagaa ccacatcgtc gcgtcggcgg tgtgatagac gccctcgttc gcgccctcgg
     5401 ggaacatgtt cggcacgagg ccgtcgcgaa tgtgctgcgc gaacatgcgc aagatcgatc
     5461 cggcttcctg cacgcgaccc gtcagcagcg tgaggccctc cagcgagatc atcgtgtcgc
     5521 gaccccagtc ggtgaaccag tgatagccgg cgatcaccgt ccatgcttcg tcgcccgacg
     5581 cccgcgcgcg aacatgatct tcgcgacgcc ccgcaggacg aatcacgaat tgatcggcgg
     5641 cgaggacgag ctccgcagcg gggccggtgc gtgcgggcgg cacggcggca tcgaggagac
     5701 ggcgacggcg ttcgagctct gccgcgagca ggtcgtcgga cggcatcgcg agcagtgtgt
     5761 cccagctctc cgtcgacgcg aacatcgaga cctgcgtgcc tggatcgagc tgcgcgcgga
     5821 agcggccggg gctgtagagc tgaccgcgcg actcgtagcc gcggctctcc tccgtcgagt
     5881 atgcgaggtc gggcacgcac gagggctcga gcacgaacga cgtgtgcgtg ccttgcatgt
     5941 gcagcttgag cgccggaatc ggcgacggcg cgatgatctc gatgcgcgag ccttccgcgc
     6001 gcagcgggta gtggtccgga accttcgtcg acaccgaatc gtcgtggccg cggaactgac
     6061 agccggggcg cagctccagt cgtacgggac caccgtcgag cagcttgtag acgaccatca
     6121 tcgtgttcgt gccgtacgag acgacgatgc gcttctcgag cgtgatgccg gcgccttcgt
     6181 agcgccacac cgggaggccg agctcgagcc gaaactcgac gaggttcatc ggcatgccgc
     6241 ggctgtacac gggcggaacc cagccgacgt cgatgcgttt gccgtcggcg agccgcagct
     6301 gcgcgggcag cgacgcgagc atcatgacgc gaccggcggg attcggaagc gcggcgacga
     6361 gcaggccgtg atagcgacgc gtcgggacgc cgagcagcgt gccggatgcg tatccaccga
     6421 gaccgttcgt gacgagccac tcttgcgtgg gatcgttgtc gatcgtcgtc gtgcgaatcg
     6481 gatcgggcac gggctcgagg gcgcttgtca tagatcgggg agctccttct cgccgctcgg
     6541 cggtggcgga tcgactcgca ggtacgcatc gcgatcgggt gcgaggagca ccgcgcagcg
     6601 agcaggaatg gcgaggcgct tcgacgtgaa cacctcgggc gtgccgtgac cgccatacgt
     6661 cggatcttcg ctcgaccact cgaggcgcca gccatgatcc ggcggcggag cgaggagcgg
     6721 ttgcggcaac acggcttggc gaagcgtcga gccgaggttg acgagcagca agcgatggcc
     6781 ggtgtggtgc caccagcgga tgcaaaacgc gcgatccgtg agcacggcgc cccggagatc
     6841 gccgtcgatg aatgctggcg tctcgcgacg aatgcgcagc aggtcgcggt gcagctgcac
     6901 gcacgatcga tcgaacgagc gctcgctcgg atcgaggatg cactcggaga acgtcgcggc
     6961 ggcgcctgga tctgggagcg ctgcttgcgc ctcgggcgtc gcgagccgag cgaattgctg
     7021 caggaactcg gcgcggcctt tcttgacgag cttgcacagc tcggagtcgt gatcgcagaa
     7081 gaagcgccac ggcttcgtcg agccggtctc ctgaccttgg aacagcatcg gaatctgcgg
     7141 gccgaggagc agcagggcgg tgagcgcgcg atacgacgac ggatccgtca gcgtgatcat
     7201 gcgatcgcca aacccggtgt tcgcgacttg atcgtgattc tcgaggaagt gcacgaagcg
     7261 cgacgccgag aggccgcgtg tcggggttcc gcgcgtgttg cgctgccacg cgaagatctg
     7321 cccctgatag aggaagctgt gctcgatcgc agcgacgagc tcctgcggag tgcccgcgta
     7381 atcgtggaag tagccgtcgg tgacgcccgt cagcgcgact cgcgtcgagt gctcgaagtc
     7441 gtcgttccag atcgcgtcga ggcccatgcc accgtcgtcg atgggtgcga ccatgcgcgt
     7501 gtcctgcggc tcattctcgc cgacgatcca gatgcgcttg ctgcgcgccg cggcacgagt
     7561 aacgcggacg atgtcggcga ggatatgcgg cttggtgtcg tcgaagatcg cttgcgtggc
     7621 gtcgatgcgc aggccgtcga agtggaactc gtcgatccag tagcgcgcgt tcgagatcac
     7681 gagctcgcgc atgccgcgcg atcggtagtt cagcggatcg ccccactcgc cgggcaagtc
     7741 ggtgcgcttc atctcgggcg cgtagacgaa gtgcgaattg ccggcgggtc cgaagtggtt
     7801 gtagacgacg tcgaggatca cgccgaggcc gagcacgtgt gcgcgatcga tgaatgcgcg
     7861 gaggtcggcg ggcgtgccgt agtggtgatg cggcgcgaac agattcacgc cgtcgtagcc
     7921 ccagccgtgg cggccggcga actcgttgac gggcatcatc tcgatcgtcg tgatgccgac
     7981 gtccttgaga aatggcaggt gggccgcggc ggcgttccag gtgccctcgg gcgtgaacgt
     8041 gccgatgtgc atctcgtaga cgacctgcgt ctcgggcctc gtgatgccct gccactgcgt
     8101 cgccgtccac gcagcatcgc gcgggtcgat caccagagaa ggcccgaaag gtccgtccgg
     8161 ttgcgcacgc gacgccggat cggcatgaag ctgatcgccg aggcggaatc ggtagcgctc
     8221 accggcgccg atgccgtcga cgaagcgtgc gtggtaaccg tgcgcctctg gctctagcgc
     8281 gatctcccgg cgcaccggac cttcgagcac gagcgtcact tgtttccacg ccggtgccca
     8341 aacgcgaaag tggactccgc cttccgcgat ctctgccccg atcgggagtc tgcgcgacac
     8401 gcccacggaa gttagcggtg cagcgcacgt gccacgagac acccgcgctt tgccaggcaa
     8461 ccgttctaga tccgcgcttt ggggcacgcc gggcgggcac gctcgcgtga tttgccggaa
     8521 tgccgtggta tagcccgcgg atgcgcaagc gtctccttgc cctcctgttg gtcgccgcgt
     8581 gtggtggcaa aacgaagcac gtcgatacgg ttccgcttcc gcctgatccg aaggtcgcgg
     8641 aagaggaaaa gaagccggtc gaagagaagc cggccgaccc gcctgcgcca acgccgcagc
     8701 cgctcgacgt tccgatcgcg acgccgaagc cgaccgtgaa gctcgttaac gcgggcaagg
     8761 gcaagaaggc cgcgctcaag atcgcgccga aggccggagc gaagcagacg atcgaagtcg
     8821 cgctcgattt catcggcggc caagacggtc cgcccgaggc cggcggcaag caggatcaga
     8881 ctgcaccgac cgtgatcctc gccgctgaca tcgagacgaa agaggtcggc accgacggtc
     8941 agtcgaagtt ccaactgacg atttcgggcg tcgaagcgaa ggacgcgccg ggctcgaagg
     9001 ttcccgctgc gcagttcaag tcggagctgc aagccctcat cggctcgagc atcgcgggct
     9061 cggtcggcgc agacggctcg acgacggggc tcacgctcca catggacgca gtcagcgatc
     9121 agaagcagct cggcgcactc gcatatctga agacggcgct gttgccgctg tggccggtgc
     9181 tgcccgccga ggcgatcgga cctggcgcga aatggaccgt cacgtcgacg gaaaagatcg
     9241 ccgaccaggt cgaggtcacg aagaccgtaa attacgagct ggtcgcgaac aagagcggtg
     9301 cctggacgat caagggcacg acgaagctca cgggcgccga ccaggagctc attgctgacc
     9361 ccgagaagcc ggacatgaag gcgaaggtcg gcagcatcgg cggcggcggc accatcgagg
     9421 cgacggtcac cgacggcgcc ctggtctcgc cggtcaagca gaagatgacg tcggacttca
     9481 cgatcacggc tcagacggtg aacatcaagt tccacctcga gcagtcgaac gcggtcacca
     9541 cgaagtagta caaccgcggg atgcgatggg gcatctcgct ggtagtggct ctggctgcgt
     9601 gtgtggctgc gtgcaggcct gtgcgcatca agccggtgca gccgaaggtc gacgagccga
     9661 agctcgacat cgtcgcgccg tggctcgatc cgagtgattt ctggccgcgc ggcacgccgg
     9721 agaagcgacg cccgctctct gacgcgctcg ccgatccggg cacaccgccg atcgtgatcc
     9781 gcaacgcgac gatcctgacc gcgacgggcc agcgcatctc gaacggcacg atcgtcgtcg
     9841 acaaaggcgt gatcgcgtac gtcggtgacg gcaccgatgc gaccacgccg gacggcgcca
     9901 cgatcatcga cggccaaggc aagttcgtca cgccgggcat catcgatgcg cacagccacc
     9961 tcggcgtcta cgcggcgccg aatgcgcagg ccaatgacga cggcaacgaa atgtcgtcgc
    10021 cggtcacggc acaggcgcgc gccgagtacg gctactggcc gcaagatccg cagatcgcgc
    10081 gtgcggtcgc gggtggcgtg acgacgtcgt tgatccttcc gggctccgca aacctcgtcg
    10141 gtggtcgcgg cttcacggtg cagatgaagc ccgcgcgcac ggcgcgagag gccgcgttcc
    10201 cgggcgcacc gtcgacggtg aagatggcgt gcggcgagaa cccgaagcgc gtctacgcga
    10261 acaagggcgg tccgcaaacg cgcatggcgg agtactccgc gttccgcagc gcattcctcg
    10321 aagcgcaggc gtacgccgcg aagcaaaacg catacgtcaa gctgcgccag cagtggagcg
    10381 agaagcggtt gcgcgcggcc gagatcgaga gcaagacgaa ggacaagaag cgcaagattc
    10441 cgggcgaggt cgcgcccgaa gcgccgcccg tcgatctcaa gctcgagacg ctcgcaggcg
    10501 tgctgcgcgg tgacgtgctc gtgcagatcc actgctatcg cgccgacgag atggcgaaca
    10561 tgatcaacgt cgcgcacgag atgggcttcc acatccgctc gttccaccac gcgctcgagg
    10621 cgtacaagat tcgggacgtg ctcgcggccg agggcatcgc gatcaacacg tgggccgatt
    10681 ggtggggctt caagatggag gcgtacgacg gcatcccaga gaacgccgcg ctgatgacgg
    10741 aggcgggtgg ccgcgcgacg attcattccg actcggcgat tggcacgcag cggctcaatc
    10801 aagaagcggc aaaggcgatg gcgtcaggcc gccgcgcggg cgtgaacatc gacgaggaca
    10861 ccgcgctcaa atggatcacg gcgaatcccg cgtgggtgct cggcatcgac tcggtcaccg
    10921 gcacgctgga aatcggcaag cgcgccgatg tcgtgatgtg gaacggcacg ccgttctcgg
    10981 cgtacgcgcg cgcggacatc gtcgtgatcg tcgggcaagt cgcgtacgac cgcagcaagg
    11041 gcatgcgcgc gagtgacttc gagctcggcc acggcgtgct cgacgtcgga atgccgacct
    11101 cgccgctgcc tgcgcccacc gcgaagacgc tgatgccgaa ggggcccgcg cctcggacgc
    11161 cggctccgca gacagcgccc gctccgcaaa cagcgcccgc tccgcaggca ccacctcctc
    11221 cacccgcttc tccgccggga ggtgtccgat gagagcgcaa gatacgatga ccgtcgcgcg
    11281 ggctgtcgaa ggtcgcagcg ttgcgatgac cgccgcgcgg gctgtcggag gtcgcagcgt
    11341 tgcgatgacc gtcgcgcagg ctgtcgaacg ccgcgagcat gcgataaccg ccgcgcgggc
    11401 tatcgaaccg cgcgcgctcg cgatcaccgc gcgtgtcctc gctcagctcg ctgtgttcgt
    11461 cgcggtcgtc gtcgttgcga caagcgcgag tgccgcgccc atcgcgatcg tcggtgcgaa
    11521 ggtctacgtc acgccggata acgtcgtcga gaatgcgacg gtggtgatcg acaacggaaa
    11581 gatcacggcg gtgggtgcgg gtggtgcgac gcccgcgggc gcgacggtca tcgacggcaa
    11641 aggcaaggtc gtgacggcgg ggctcatcga ggcggtttcg gcggtagggc tcgtcggtgt
    11701 cgatctcgag gctacgtcgg tcgacgggcg attcgggccg acggatccag tgcacgatga
    11761 tcccgtgcat gcgtcgtacg acgcgcgcga tgggtttgcg ccgcacgacg tgacggttgc
    11821 agttgcgcgg accggcggca tcacgctcgt cgtcgcggcg ccgggcggtg cgctcgtcgc
    11881 tgggcagtcc gccgcgtaca cgctcgacgg aagtgccgag gcggtgaagg cgcaaactgc
    11941 gatgcacatc gagctcggct cgaacgcggc gagcggcggc tctcgcggca agacgctcga
    12001 gctgctgcgc gagatcctcg acgacgcgcg cgcgtatggc cgcgacaagg gagcgtacga
    12061 gcgcaacgcg aagcgccact tgatcgcgga tcggttggac ctcgaagcac tgcagcccgt
    12121 gctcgcgggc cgcatgccgg tgatcatcgc cgccgacagc gaggcagaca tccgcgcggt
    12181 tctgcgcatc gcgaaggact tcaagctgcg cgtcgcgatt accggtggcg cggaggcgtg
    12241 gcacgtcacg aaagagctcg cggccgcgaa ggtgccggtg tttgtcgatc cgatgcagaa
    12301 cttgccgggc agccttgcgg cgagtgacgt gcacgatgac gcggcgaagg tgctcttcag
    12361 cgagagggtc accgtcggca tttcgacgct cggtgccgcg ggcatggcgc gcacgctgcg
    12421 gcagctcgca ggcaatgcgg tcggccacgg gctgccgtgg gcgagggcgc tcgcggcgat
    12481 cacgactgtg ccggcgcagc tctatggcct caccggtcgc ggcaccgtcg agaaaggcag
    12541 catcgcggac gtcgtcgtct ggagcggcga tcctctcgag gtgtcgaccg ccgccgagac
    12601 ggtgatcatc ggcggcgtcg tgcagtcgaa cgagacgcac cagacgaagc tgctcaagcg
    12661 ctatcgcacg ctgcccgcga agatgaccgg caactaggat tggggtccgg gagccggact
    12721 agcgcacttc ggcggcggct ttgagctgcg ggaacgtctt cgcgaagctc tctttgtcga
    12781 gggctttctc ccacgcggcc tcggccttga tcgtgcctgc gcggacgagc ttctcgagcg
    12841 ccgcgtccat cgcctgcatg ccttcggaca tgccttgttg gatcagcgac gcgatctgct
    12901 gcgtcttggc ttcgcggatg acagaggcga gggcgaggct gccgagcaag atctcgtgca
    12961 cggcgacgcg gccttggccg tcggatttcg gcaggagctg ctgcgcgacg acgccggcga
    13021 gcgagtctgc gaggaggccg cggatttggg cttgctggtc cgcggggaac gcgttgacgt
    13081 agcgatcgat cgtggcggcg gcggagttgg tgtggacggt cgcgtagatg aggatgccga
    13141 agctcgcgag ctgcagcgcg gccttcatcg tttcggcgcc gcggagctca ccgacgagga
    13201 tcacgtcggc gttttcgcga cctgcgctgc gaatcgcgtc gaggaacgac gggacgtgct
    13261 cgccgacctc tttgtgcgtg atgaggcact tgcgcggttg gtgcacgaac tcgacgggat
    13321 cttcgatcgt gaggatgtga ccgggccgcg tgcgattgat gtgatcgatc atcgcggcga
    13381 gcgtcgtcga cttgccgctg ccggtcgggc cggtgacgag cacgaggccg gcgcgcaggt
    13441 cagcgagctt gcgaatgccc ggcgggacgc ccagctcgtc gagcgtcttg atgatcgtcg
    13501 ggatggcgcg gaacacggcg cccgtgccgg tgagcttgcg caggtagttc gcgcggaagc
    13561 gcgccttcgt gccgtgcgcg tgcgcgaagt cgaggtcgcc cgtcgtgtcg aacgtgttgc
    13621 gttgctccga cgagagcagc ggcatcagca tcgcttcgat gtcgccgctc gtgagctcgc
    13681 gcgcgccttc ggcgacgagc tggccacgga cgcgcagcat cggcgggtac ccgggcgtga
    13741 ggtgcaggtc ggagccaccg cgctcgagca gcttgtcgaa cagcgagttg atcgcggcgg
    13801 cgctcatttg accttccaga gcagctgcgt cttcgaatcg tcctcgatgc ggcccgtgcg
    13861 cacgagctcg gcgatcgatt cttcgaggcg aatcatcccg aacgcgcggc cgcgctgcat
    13921 caggttcggc agctgataca tcttgtcctc gcggatcatc acggcgagcg gcagcacgcc
    13981 ggtgacgagc tcgacggccg gcaccacacc gccgccgcct ttcttcggca gcagccgctg
    14041 cgcgacgatc gcgcggagcg cgccggcgat cgacgagcgc acttggctgt gctcctcggg
    14101 agggaacatg tcgatcaggc gatcgatcgt cttcgcggcg gacggcgtcg acatcgtcgc
    14161 gagcacgagg tgaccggttt cggcggcggt gagcgcgatc tcgacagact cgcggtcgcg
    14221 caattcgccg atgacgatga cgtcggggtc ttcgcgcagc gatgctttga gaccgcgcgc
    14281 aaagctcgtc gtgtgacggc cgacttcgcg ctgcgagacg acggccatct tgcgcggatg
    14341 cacgatctcg acgggatcct cgatcgtcag gatgtggtgc ggctttgtgc tgttgacgag
    14401 atcgacgagc gcagcgagcg tggtcgtctt gccgtggcca ctcgggcccg cgatcacgac
    14461 gaggccttga tgatgcgaga cgaccttcgc gagctcctgc ggaaggccga gcttgtcgag
    14521 cggcagcggc ccgggcatcg cgagccggaa cgtgcccttc aagccctgct gctgcttgca
    14581 gaggttcgtg cgaagccggc cgccgtcagg cgcttcgagc gcgaagtcga cgtaaccgac
    14641 gcggttgagc gtcgtgcgca tctcgcgatc gaggagcggc aggagcaagc cctcgacttc
    14701 cgcggctgac agcggctgcg tgccgtcgag cggaaggagc tgaccgccga ttcgcgccga
    14761 cgtcacgcgt gctgccgcga tgtgcagatc acttgcgccc cgtgcgcgcg cggcttcgac
    14821 gagcgccgcg agctcacccg tcgccttcgg cacggcgctc gattgcgcgt gcggcaccgt
    14881 caccggatgc gtcgcatcgc tcgcgctcac cggcggtaga gcaatgctcg gcgacgacgt
    14941 atgcgtaggc gcgtcgatct ccacgcgctc gcggatcgag gggatcacga tgcccgcaga
    15001 cgacgaatgc gtaggcgcat cgacgtcgat cgccgtgggg atgccgtgtc cgggtgatgc
    15061 aggttgtcta gcggcgccgc gctgcgttcc gagtgccggc gcattcgccg aaccgagcgc
    15121 tgctccgacc ggcgcgccga gctcgatgtc gccgtcgagg tcgagcgcgt cgaagaggcc
    15181 actcggcgag aggtcgcgcg ttggagcagt cgctgggccg agcgtcgatg cgctcgcggg
    15241 tgcgggagac gacgccgtgc tgaacatcga cgcgccggcg gggatcgcct tcgacgcacc
    15301 gacgagcgac gcggggctcg gcataccgat gtcgatgctg ggccgcttcg tcggcgactt
    15361 cgcgcgagcg gccgtcggac gtttcgcgcg cccggtggtc ggagtctttg cgcgtgcact
    15421 tgtcggcgac ttcgcccgcg acgacgtcgg cgactttgcc cgtgacgagg atggcgatgt
    15481 tgcgcgagtg gccgttgggg acttcgcgcg agcgccggtc ggggacttcg cgcgtgcgcc
    15541 cgtcggggac ttcgcgcctg cgcccgtcgg ggacttcgcg cgagacgccg ttggcgattt
    15601 tgcgcgagcc gcgctcgaag actgtgcatg tgcgctcgtc ggagcttggt ccggcgtgcg
    15661 tgcagctggc tcggcgatgc gctggatcgg catcgtcgtc gcgacgccgt cgggacgcgt
    15721 caaccggacg agcacctcgt cgccgcggcg gccgatctgg acgcgaactc ggcgcgcacc
    15781 gatctgcacg tcgaccggtg ccgccgtcga gtccgcctcg ggaatcagcg cggcgacggg
    15841 cgaaccgcgc agcatgttcg tcagctggtc gagcgagacg ggtcgcgccg tcaggttcgt
    15901 cacgccgctc gcgtttcgca tcgcgatcgg gcggccgaca ccgaggatga tctcgctgac
    15961 gcccgggcgc tcgagatagc cgatgagttg gtcgagctga ctcagtcgga gaattgtcgc
    16021 atgagtcagc tcggcggtgg cgtagccgcc ggggtagcag cgggtgcctt gtaggcaggc
    16081 gcgtacttgc cgaggacctt cgtgctgcgc accgcgtcgg gaagatacgt atcgaggcgg
    16141 ccgacgtacc acgtgcccgc gagcacgagc agcgtcgcgc ttgccaggta caagcgccat
    16201 ggcggcgtct tgtcggcgaa cggatccgag agcacgcgct tgctgtgctt cgggatcgcc
    16261 gcgagctcgg tcatcgctgc accgaacgac acgttgatgc gagtgcgact gttgatcgcc
    16321 cagccattcg cgtcgaggat cggaccgagg ttgcgtcggc gcaccttcag ccaggcgagc
    16381 agcatcgacg gaccgcttat catcagcagc agcgcgagga tcccgagagg tagccactta
    16441 ccgagaccaa acatcgtcgc gagcagcgca ccgacgagag tgccgatgcc gccgatcgcg
    16501 acgccaatcg cagcgaccgt gccgagatcg atcttcttcg gtgccgcctt gggatctgct
    16561 gccggggcaa ccgcgtcttt cgtcgccgcc ttgtcgacgt gcgcgatctg cgtgccggct
    16621 gccgtcatcc gtgcgctggc agcatcatcg gcggcggcgg cgcgcttcgt gacgttgtct
    16681 tccacgactt tgacgagctt cttgtacggc gcccagaacg cttcgcgaac gctgatcggg
    16741 ttcgtgacga tcttcgtgat cgtcgcgtcc cagtcgtttc cgtcgcgatc gtagaagatg
    16801 ccgttgcggc cgacgaacac gttgtccgaa tcgccgttcg tcagcgcagc tgcgatttgc
    16861 ttcgtcacgc cttcgcgcgt gatgtcgcaa tacaggaggc acgcggccga cgacgcggcg
    16921 agcgcaccgt gcttgccagc gtcggtgacc ggaatgcaga ggtggagtgc gcgcgcgtcg
    16981 aggtagagcg tgcccgcttg gaacacgccg tccttcttcg agtagaagtc gctgaagttg
    17041 acgaagttgc ggacgatgcg gccgaagtcg cgctggaacc gcacggcctt cacgacgctc
    17101 gtgatctgct cgtactccgt ggtgagcgcg ctgtcggctg cgatgaggtc ggcgaccttc
    17161 gcgcgaatct cgggcgcggc gagagccttg atgcgatcgg gcgagagtgc gccgactttc
    17221 gtgtcgggct tgcccgcgta ccatgcgtcg taggcggcga gcttgtcgca gactgccttg
    17281 tagctcgtcg gcgtcagagc gtcacatgcg ccgaggatcg gcgtgacggt cttgtcggtg
    17341 aacgtcccga gcgcgtcgat ccacgccggg tttacgccca cgcgtagcgg cagccgagat
    17401 gcagtgtcga cctttgcgag cggcaatgcc ctcagcgacg caggatcgag cgacgtgagc
    17461 ttgatggtgg cgggcaccgt ctctgggtcg aacgcagcga gcttgcagag cgtgaagtac
    17521 tcctcgatct tcgccttcac cgcgcggagc gcatcgccgg ccgcgtcggt cgcttcgccc
    17581 attgtcttcg ccggaccgac ggccgcgagc catgccgctt gcttgtcgac gtcgccgaag
    17641 aactgatcgg cgagcgcctt gtcgaggccc ggcttgccgc tgcgatcgtt gaggctgcca
    17701 acgcatgcga tcgcatcttc gatcgcttgg cgcacgggcc ccgcgtccgc agacgctgga
    17761 atgatgatgc cgtcgccgtt gagcaccgtg tcggcgaatg ctttcgtgac ggcgatcgtg
    17821 tcctcgatcg tgatcgtctc ggcgtccttc ttgccgaggt cggcgagcat gcgcttcgcc
    17881 gcggcgacca ccttcgcatc cgagaacgcg gcgaggtcga cctcgtcgga gctcgtcagc
    17941 agctcgctcg gattcttgaa ccactgcttg atgtctttga cggcggcgag gatgtcctgc
    18001 acgcggatgc ggccatcttt gttcgcgtcg aggagctcga gcgtcgcagg atcgacgtcg
    18061 atgcccttcg tcggcatcgc gagtgcgacc cacaacttct ggtcgagctc tggcaacgcg
    18121 agcaggtcaa acccgtcgcg aagcgaaacc tgatcgactg cccctgcccg gaagaactgc
    18181 caccgatgct gcattgcgcc gcatcgtaat cgatctactt cgcgatcacg cggtccgtcg
    18241 tgatcatgaa aacactggtg taggcggcgt tgtacttaca gctggcgagc gtcggaccgc
    18301 cctgcgatcc ggagctgaca cacgtggtac cgacgcagcg ggtgcagccg atgatcttgc
    18361 gagtcgcgtc ggactgaaac gtgtacttcg cgccatcgcg cgacgcatcg ggttgggtgc
    18421 ccttgacctg cgtgaagagg ccgaacgtct tgcagccgtt gacgaacacg tcgagcagcg
    18481 tgtgcgaggc agaataattc tcagcgcagg gagtgcttcc gagcatcacg gcaggcaccg
    18541 cgctgccatc cagcgactcc gccgtcgtgt tgccacagac ctcgccgctc agcttcgtga
    18601 tgttgccgtt ggtgtcacgc gtgacggtgc ccatcgactc gaagctggtg agtgctggat
    18661 tcaggtgctc ggcggcaata tggccgggcg tcatgtcgtc cgtcgatacg gtcggtgcgg
    18721 acgcgccgtt gatgccaacg cggacctttg cgtcgaccat gtcgaagtag cagatgacgc
    18781 cggagaagct cgcgctgacg acgacctcgg caggtcccgc ggtgagcacc ttcgctgcga
    18841 tcgcgccggg catctgcgtc ttcggtacgc gcgcgctgtc gatgtccgct gcgttcgtcg
    18901 tgtaccacca gtcgagatcg gtgttgccgt tatacgtctt cgtgctggtc acgggcggcc
    18961 ccttgatcca cgcaacgctg acggacgcgt cggatgtgcc cgagaggtca tcgagcccca
    19021 agaacgcgaa gatgactgcg atcgaaccat cggtgacgcc cgacctgaac gcgtctgtaa
    19081 tgttcgtgcg caccgagctg ccgaccatcg actcgcccat cgcattcttc gtacagaacg
    19141 cgtaggggcc cgtgaaagga atcatgagct gattgacgcg atggacctgc tcgaacttgc
    19201 acgtgccgtt gcagccgtcg aggttcgtca ggttgccgtc atcgcactgc tcgttgccgg
    19261 cgcggattcc atcgccgcac aggatcgttc ggcactggct gtcgcacgtg gctgtgttgc
    19321 cgggctcgca cgactcgccg ttgagcagat cgatgtagcc atcaccacac gtcgagttcg
    19381 tgcacgcttg agagcggcag attcgcagcg tgccgcacga cgaaccgtcg gtgagcgcgg
    19441 tacccgacga gcacttctga ccggccttgc cgttgacggt cacgccgccg cacgtctcgg
    19501 tgccgttgca cgcattgccg tcgctgcacg aattcggcga ggtggtgcac gagaactgac
    19561 aggacggctc gcacccggag ccagccgtgt tcatgccgct gccgaagtcg cattgctcgc
    19621 ccgcctcgac gacaccgttg ccacacaccg ccgccggcga cacgcagctg ccgctgctgc
    19681 acacgaggcc gccaccgcca cagctcatcc cattgagcgt gctgtcggcg acagcctggc
    19741 aggtgtgctg caccgtgcac tgctctttct tgcacgccgg cgcggtaccg cagtcggtcg
    19801 ccagggtgca gacccattgg cagttgctct tgcagccgtc gttcgcggtg ccgttcgccg
    19861 atccgtgatc gcacgcttca cccagctcga cgagcgagtt gccgcacacc ggctgcgtgc
    19921 acgtaccctg tttgcagtag ccgcccgttc cgcacgcagt gttatcggcg agtggagtgc
    19981 ccgcgctgca cgtgtgcgtc gtgttgttgc aggtgccttg gcccttgcat gaatctgtcg
    20041 gcgtgcagtt gcgcgtggcg tccgtcgaca cgcagctgaa cttgcagttg ttctcgcagc
    20101 cgtcgccgag caactgattc gcgtcgtcgc actcctctgg tgccgtcgtg aagctgtcgc
    20161 cgcactggcc tgtcgcgcat gcattgttga tacagatctt gccgctgccg cagctcgtgc
    20221 ccggtgccgc ggccgtaccc gcggtgcaag ccataccgtc gtgaccgtcg acagtcacgg
    20281 atgcgcatag ctcggcgccg ttgcagagat cgccgtcgtc gcaactgttg ccgctgcacg
    20341 agaacgtgca gttcgcttcg cagccagcgc ccgctgcggg atcgactccg aagtcgcatt
    20401 gctcggtccc ttcgacgact ccgttgccac acgacgaaga cgtcaccgca gcgtccggcg
    20461 cgtcgaccgc ggcgtccggc gtgttgccat ccggcatcga gctcggggcc ttatcgccgc
    20521 aacccacgag cgcgagcacc accaggacgc gaacggtcca agaacccatc gtccccgatg
    20581 ataaggggcc gacgcacgcc tcgtcgatgt ggctacgccg cgtacacgta gcttccctac
    20641 tgcgctgcgc cacggacgag ctcgatgagc aggcccgtac aacgatcact gatctgcagg
    20701 gttgtcgagc ccgtgctcgc gaccgcggtg agacacgtcg ttacgctgct cgggaacgag
    20761 acccgcggcg gcgcgccgta gatcgtaaat acgttcccgg caccgtcgac gacgccattg
    20821 tcgccggccg cgagcatgcg gacggttgtc gtcgagctga cgaacacgtt gccaaacgaa
    20881 tcgcaggtca caccacgcgg agccgcgacc gggaatgtgc gcgcgggact gccttcaccc
    20941 gacgacgcga acacgccatc gcccaaaacc gtgctgattg tctcggaacc gctgcctaca
    21001 ttctcgagcc tgcggacgcg gttgttgccg gtatcggcga tgaacatgtt gccgttcgcg
    21061 cagcgggtga gcgcggtcgg tcgatacagc agcgcacccg cagcgagccc gccgtcgccc
    21121 gcaaagccga gccggtgagt cgtgttgacc agcgtcgaga cggtcgctgc catcgtgttg
    21181 aggacgcgga tcgcatggtt gtcggtgtcg gcgatgtaga gcagcccggc gtcgtaatag
    21241 agcccggtgg gtccacgaaa gcgcgccgtg cccgcagacc catctgcgaa cccggcaacg
    21301 cccgcggtat tggcgagcgc cgcgatcgtc cacgtcgacg gatcagccgg attcacctgc
    21361 gtgatgacgt ggatgcgatt cgacgacgtt tcactgacga agatgcggtg gttcgcttcg
    21421 tcgtacgcca cgccaccgac cgagccgaac gcttgcgtgc ggaaccgcgc gagggtcgac
    21481 gtcggcgaat tctgcggata gcgacctgcg accgcgtcga cgcggctgtt gcggatgatc
    21541 tcgagcgtgc cgcttgctcc gccggcgacc agcgtcatgt cggaggagat cgcgagctgc
    21601 tgcggatccg agagcctcgc gctggcgacc ggtcccgtcg agtcggggtc gaccttgccc
    21661 gcgacgttgt ggatcgtgcc gtcgagctcg acgcgacgca cgcgccgatt gcccgtgtcc
    21721 gcgacgagga gtcggttctg cggatcgatc gcgataccaa gcggggtgcc gagctgcgcg
    21781 cttgtcgccg gtcctgcgtc gccattgggg ccggccgcgc cgcccgcaat cgtcgtgatc
    21841 gtaccgttgg cctggatgcg gcgaatgcgc atgttgttgc tgtctgcgaa cacgacgagg
    21901 ccgttcgcgt cgatcttgat gccgtatgga ctgtcgatct gagcgcttgt cgccgcgatt
    21961 ccgtcaccat tgaagccggc agttcccgta ccggcgatcg tcacgatggt gccgttgccc
    22021 tcgatccggc gaacgcggtt gttcgacgta tccgagaaca ctacgcggcc ttgattgtcg
    22081 acggccacgt cgtagggata gccaagctgc gcgctcgtcg ccgcgatgtt gtcaccgttg
    22141 tagccaagcg ttccagtgcc cgcgatcgtc accatgttgc cgttcagctc gacgcgccga
    22201 atgcgctggc cgtacgtgtc ggcgatgatg aggcggccgc tggcgtcata cgcggtgcca
    22261 tacgcgcgcg agatcttcga cgacgtcgcg ctcaggttct cgccgttgta accggacacg
    22321 ccggttccgg cgatgcgcgt gatcgttcca ttcgtctcga tgcgacggat gcagaagttg
    22381 aacgtgtcta tgaacacgac gcggccttgc ggatccaatg acacgtagaa cggatgtccg
    22441 agctgcgcct gtgtcgcggg cccgccgtcg ccggagaacc caaacgtgcc tgcgcctgcg
    22501 atcgtcgtga tcgtgccgtc gagcgcgacg cggcggatgc gatcgttgaa ggcgtccgcg
    22561 atgatgatct ggccctgcgc gtcgacggcc acgccgtaga ggttcgtgag cagcgcgctg
    22621 ttggcctggc ctccttcgcc gccgaggccg aaactgccgg tacccgcgat ggtgtcgatc
    22681 gtcgccgggc tcaccgtcgt gtcgatgcgg cgaatgcgct ggttcgacgt gtccgcgatc
    22741 aagacgcgac cggatggatc gacggcgacc gagagcggat tcgcaagcgt tgcggctgcc
    22801 gcgggtccgc cgtcgcctgc gaagccctgc gttccggttc cggcgaccga cgcgacggtg
    22861 ccgttggtca agaccttcgc gatcccctga ttcccggtat cggcaacgta gaccgtgcct
    22921 tgcgcatcga ctgcgatgcc ggtcgggaag gacagacccg acgtgacggt ggtgatgact
    22981 ccggcggcat cgacgcggcg aaccgcgttg ttctcggtgt ccgcgacgag catgcgatcc
    23041 tgcgtgtcga tcgcgacagc gaacggcgtc tgcaggctcg catcgacggc aggtccggcg
    23101 tcgcctgaat accctgggac gccatttccc gcgacggtgt tgatcctgcc gtctatgtcg
    23161 acgcggcgaa cgcgctggtt ctccgtgtcc gcgatgtaga ggcgaccgag gccgtcgacg
    23221 gcgagaccgt gcggtgcctt caacatcgcg aggatcgcgg gtcggccttc gtcttggatg
    23281 tcgtagccac cgacgccgac gcccgcgacg gtcgtgatcg tgccgtcgag atcgatcaac
    23341 cgcacgacgt ggtttccggt gtcggcgacg tacacgcgac cgatgccatc gactgcgatc
    23401 cccgagggcg ctgcgagctg cgcgcttgtc gctgctccgc cgtccccgac gtagccggcg
    23461 gcgcccgtac ccgcgagcgt ctcgacactg ccgtctgctt cgatgcgcag cacgcggtgg
    23521 ttcgacgtgt cggcgataaa gacgcggcct tggccgtcga cggcaatcgc attcggcgcc
    23581 gccaatggaa tcgcggtcgc ggcgatgcgt gagccgacga tcgcagctgc cttccacgcc
    23641 gtttgcccgc acgcatcaca gccgtcggcg gggtttttat taccgtcgtc gcactgctct
    23701 gcaggatcca cgaccgcgtt gccgcactcg tagatctgcg tgcaatcgcc gctgcacgtc
    23761 tggccgagca cggcgtcgat gccgacgtca ccgtcgcaga cttcgccaac gtcgatcctc
    23821 gcgttgccgc agagtccagt gtggcatgcg ccgccctggc agatgcccga gccaccggtc
    23881 gcggcacgac agggatcgcc gtcggagagc ccgacgcacg cttcgacgtc ggcaggcgcg
    23941 gcgcaaccac cggcggtgca cacgttaccc ggagggcaca cgagatctcc gcacggaacg
    24001 agcggagact ccgcgcaggc ggcgaatgcc agtcctgctg cgagcgtcgc gagacgcgct
    24061 gcacccttca cggacgaaaa tcgtaacacc tctccgcagg cgacatggac gtacatcgcg
    24121 gagccggcgc gacgcgcgat acgatgcgtg aagagtgtct gggccgaaga ccaccgacgg
    24181 gaacgagctg ccgaccgtca acatgcccct cggtcctgcg cgcaagacgt ccgagtcgac
    24241 ggaagtactc ggcaaccagg tattcgcgcg gctctcggtg agcggccaca cgcgtgtcga
    24301 caagcggcct ccgctcgact tctcgaagaa caagcagatc ggccgcttcg agatgatccg
    24361 cgagctcgcg cgcggcggta tgggccaggt cttcctcgcc cgcgatacga agctcggtcg
    24421 caaggtcgcg atcaagttct tgctgcacga cgaccctgac ttcgtgcagc gctttctcgt
    24481 cgaggcgcgc gcgacggcgc gatgcacgca cgagaacatc atcacgatct tcgaggtcgg
    24541 cgagcacgaa ggcttgccgt acatggtgct cgagtacctc gagggcaaga cgctctcgca
    24601 agtgctcgag acacgcccct cgcagcagca gttcacggag ctgatgattc ccgtgttgcg
    24661 tgcgctcgag cgcgcgcatg cagacggcat cgttcaccgc gatctcaagc catcgaacat
    24721 cttcgtcacc gaccgcggtc aggtgaaggt gctcgacttc ggtgtcgctc gtctcatcga
    24781 tcgcgccggc gagatggtcg ccaacgtgtc ggcgcacgag ctgcggctcg acgagcactc
    24841 gagctcggtc acgcgcgcgg gcagcaactc gctcgtcggc acgatgccgt acatgtctcc
    24901 cgagcaatgg ggagcgggcg aggtcgatca cctctcggat ctgtgggcga tcggcatcat
    24961 gttctggcgc gcgctgacgg acgtgcatcc ggcgggaacg atgcatcccg agaagctgcg
    25021 tgcgcgattg ctcgatctcg tcacgccgct gccgggcctg cgcgatcaga acccgggcgt
    25081 tccggaagag ctcgcacaca tcgtcgacaa gtgtctcgcg aagaatcgag agcaccggta
    25141 tcagaacgcg agcgacgtcc tgcacgactt gcaggcattc gcggcgccgc gtgatgtccc
    25201 gcgtgggacg gagacgtgcc cgtatcgtgg cctcgccgcg ttcggcgaag acgacgcgaa
    25261 gttcttcttc gggcgcagca acgagattcg caccgcgctc gctcagctcg acagctggcc
    25321 gttgctcgcc gtgattggcc cgtcgggcgt cggcaagtca tcgttcgtgc acgccggtct
    25381 cgtccccgcg attcgaacgt cgggaagttg gaatgtgcgc gtgctgcgtc ctggccgcgc
    25441 gccactcctc cgcctcgctg cggtgctcga agaagcgctc gcaacgggtg agcaatcgaa
    25501 cgatctgctc ggtcagctga aggacgcgcc cggcctgttc ggcaagatgc tgcgcacgtc
    25561 ggcggcgcgt cggaaccagc gcgtgctcgt cgtcgtcgac cagctcgaag agttgttcac
    25621 gctctgcgac agcgacgagc atcgcaagat cttcctcgcg gcgctgctcg ccgccgcgga
    25681 cgacccgagc tcgcccgtgc gcgtcgtgct gtcgatgcgc gccgactttc tcgatcgact
    25741 cgcgggccac aagcacttcc tctcggagct ctcgcgcggc ctcttcttcc tgaccgcgcc
    25801 cgatcacgaa aacctgcgcg agacgctgat ccggccggcg gagcttgcag gctattcgtt
    25861 cgaggacgag cgcatcgtcg aagacatgat gctcgctgcg tcgagccgcg gcgcgctgcc
    25921 gttgctctcg tttgcggcga cgcggctgtg ggacgcgcgc gatcgcacga agaagcagct
    25981 gacgagcaag gcctacgagc agatgggagg cgtcggtggc gcgttcgcgc gtcacgcgga
    26041 tcaagtcgcc gcgtccgttc cgccgcagag ccagatcttg ctgcgcgcga tcatgtcgcg
    26101 tctcgtgacc cccgatggca cgcgcgctgt cgtcgacaaa gaagagctgc tgtcgatttc
    26161 ggacaaccgg caggacatcg agcagatcat cgacacgctc gttcgcgcgc gtctcatcca
    26221 cgtgcagacg gacaacgaga cgacgaccgt cgagatcgtg cacgagatgc tgatcaccga
    26281 atggccgacg ctcaagcgct ggctcgaaga cagccacgtg ctgcgcagct tcctgcacga
    26341 gcttcgccaa gcagcgcggc agtgggactc gcacggccgc tccaacgatc acgtctggcg
    26401 tggtgcacct gcacacgaag cgttgtcgct cgcgaagcgg cacgtactcg agctgtctgc
    26461 cgtcgagagc gcatatctgc gcgctgttcg cacgcaagcg gcccgcgcta ggcgcacgcg
    26521 cgtgctcgtg ttcgcctcgg tgctcgccgt cctcgtcgtg atcctgttcg gtggtgcgtg
    26581 gttcacggtg cagctcgcaa aggcgaacga agaagcgcgc gacaaggaag cgaactggca
    26641 tcacgccgca caggaagcgc aagcggcaaa ggccgacctg caacagaagc tcgacatcat
    26701 caacgagaaa gagcggctgc gcctcgcggc tgaagcctcg gcgaagcaag cgcacgaaga
    26761 ggtgctgaag gcagacgagg gcctgaagct gtcgcaggaa gagctcgaga aagcgaacgc
    26821 agagctgcgg cgcgcgcttg tcgaggcgca gaacgagaag caccgcgcac aaaacgcagc
    26881 tgagctcgcg aagaaatcga gcgacgaagc gaagcaggcg aaggcactcg ccgaggcggc
    26941 ggccgcgaag gaaaaggctc gcgcagacaa gctcgagcaa gaatccaaga gcatctacaa
    27001 caaagacctc cgacggaaac cgctcggcgg cggaagcggg caatgagagc gctcgtcctc
    27061 ctctcgctcc tgatcgccgg cgttgcccac gcggattcgg ttgcgccgtg ggagcaaggc
    27121 atacccgacg cgcagcaggc gaaggcaaat gcgctgttcg cggaggccaa cagcctgttc
    27181 gccgagaagg cgcatccagc ggcgctcgag aaatacaaag cggcggtggc ggtgtgggac
    27241 cacccgatga tccgcttcaa catggccgtc acgctgattc gcctcgatcg catgctcgaa
    27301 gcggcagacg agctcgagaa agcgttgcgg ttcggcgcgg caccgttcac gcccgagctc
    27361 tacgaacaag cggtggacta tcaagcgctc gtcatgaagc agctcggcac gatcgaggtc
    27421 cgctgcgacc agcccggtac gcacgtgctc ctcgacggca agcccttctt cgacgcgccc
    27481 ggtacgaagc aagtgcgcac gacgagtggc gagcactcga tcgtcggcga gaagcgcggc
    27541 ttcatgacgg tcgcgcgccg gatcgtcgtc ccgggtggca agactgcgaa ggagacgctg
    27601 cagctgttgc cggtcgagag cgcgatcgtc gtcgagtaca agcaaccccg ctggatgccg
    27661 tgggtggcaa cggcgacctc agccgcgatc gtcggcagcg gtgtcgcgtt cgtcgttgcc
    27721 ggcaaccacg agatggatcg ctttacggac gcgttcattc gcgtgtgtcc gagcggctgc
    27781 gaagctgacc tctcgatgca ccgcgatctc gccgaccagc aggactccgc gatgctcaaa
    27841 acgaagatcg gcgtgtcact gctcgtcggc ggaggcgtcg cgatggtcgg ctccatcgtg
    27901 tggggcgtga cgaatcgccg aacgcgccgc atcggtcctc gcgtcgaggt ctcgcccacc
    27961 gcatcgggcg cctcggcgag cgcaagctgg acgttttagt tactcgatgt cgacatcgag
    28021 gtcgtagtcg atcttcatgc cgccatccgg caagcgctga acgcgaacgg gcgcgcgcgt
    28081 cggcggaaca ggtggcggcg ctgcgaggtc atgctgatgc cagtgcggcg gcgtgggcgg
    28141 cgtaggcggc gtaggcggcg taggcggggt cggtgggacg acgatcaccg gcatgacggg
    28201 catgggcggc atgggcggca tcggcggcat gggcggcatc ggcggcatga cccacgcgcc
    28261 gcgaaagccc ttcttgccga gcttgaagtg catcttctgc agcttcttct gcagcttctc
    28321 gagcttgcgc tggagcttgt cgttcagctg atcgagctgc tgctgcagct ggatctgcat
    28381 ttggtcttcg tcgaactgga tgtcgacgtt gaatgcgccg tcgtcatcgc cgtcgccgtc
    28441 gtcgtcgtcg tcgccatcgc aattgccgcc gtggcccata cgcgacacat cgcgggccat
    28501 ctggcgttgc gcgcgttcca tctcgcgttg cgcgcgctcc atctcgcgtt gcgcttgctc
    28561 ctgctctcgc tccgcgcgtt cctgctctcg ctccgcacgc tcctgctcgc gctcggcgat
    28621 catctgagcg cgatcggctt cgagctcggc gatctgctcg gcgcgctcag cttcacgctc
    28681 cgcgatctcc tgtgcgcgct ccgccatctc ctctgcctgc tcggcggcag cttccgcggc
    28741 ggcctcggcc gcacgttccg cggcttcctg catctcgtct tcgtcgtcct gatcgtcgtc
    28801 gtcgtcgtca tcgcgatcgt cgtcatcgtc gtcgtcgtcg cgatcgtcgt cgtcatcatc
    28861 gtcggactgg gcgacgcggt cgtcgtcatc atcgtggtcg catgcgatcg cgcgcgtggg
    28921 gtgggcgacc agcagagctc cgaccaagga cgggacgagc agcatgaacg agcgcatgaa
    28981 tcctcctggc acccaaagag ccgcgagacg ccgcgccggt ggacacggaa attcagtccg
    29041 acctaatttc ctgcgcggcg gcgaaaccga gaaattcggt ccgaccgaac taatcgacga
    29101 cgcgggcggg agcgacgggc ggagcggcgg ggcggggcgg ctgcatcacg aggatcgcga
    29161 tcgacacccc ggcggtcgcg acggcaccga tgacgaccaa ggcgatggac gggccgaaga
    29221 accgcagaat cattggcagt tcgcggatcg tacgcaggac atcgaggatc atcgggccgg
    29281 cgcaggtcag cacgatcgcc gccggccact tgtcgggcac gttgcgccac atcgcgaaga
    29341 acgcgacgcc cgacgccgcg atcattccca tcagtgacac gccccagaac gagccgagcg
    29401 agatgtcgag gctcttcgcg agccaggaca tgccgatcag cgagcccagc gcgcaataga
    29461 agaggaccgt ccagcgccgc ttcacgctcc cgagcatgac acatgcggcc ggaagttgat
    29521 ccggcacgaa gcgaggtagc cgaacgttaa agcgtgaggc gcggagctcg ccgcgcacgc
    29581 tcgaggccgc gaagcgtcag gcgcggagct cgccgcgcag ccgcgagaca cgatcgtgca
    29641 tcgctttgcg agcttcgtcg gggccgacga tccacgcgtg gcggccgagg ccgagcaccc
    29701 agcccgtcac ccactcgaag ccttcgcaat cgacgagcag ctcggcggag ccatcgtctt
    29761 gcatcgtcac ttcgccgacc ggccagctcg caccgatgcg cgtgacggca agcggatcga
    29821 gctgcacgcg gaccgtgacg gcatcggccg acggcacgta gaggcgctcg cggcggtagg
    29881 cttcgagatc gaaatcggct ggacgatcga atcgctcacc cgtcgtcttg atggcagcga
    29941 tgcgatcgat gcggaacgtg cgcacgtcct tgcggttgtg gcagtggccg acgaagtacc
    30001 actcgccggc gtgatgcacg atgccgtacg gatcgaccgg acgatgctcg ctctggtgac
    30061 gcgacgccga cgcgtagtcg atctcgacgc gctcgcgctt gcgggcggcg gtgacgagcg
    30121 tgcgaaggtg cgacgcgacg gcggcgtctg gctcggcgac aaccgtcgac gtcgcgagcg
    30181 attgcgcttc gtttgcatcg cggccgagcg cggacagcag cttgcgttcc gcggattgca
    30241 tggcggcgtc gaatggcgcg attccgctct cgcgcagcgt gcgaatgccg agcaggagcg
    30301 agcagccttc cgccggtgtg aggcgcaacg ggcgcgtcag gcggtgcgcg agatcgacga
    30361 acacgcggcc ttcttcgacg gagacgagca ggtactcgcc tggatcgccg tcgggcggac
    30421 cgacctggga gagcagatcg agatcggcga gcagctcgtc gcgctcggcg ccgatcatct
    30481 tcgcgagttg ctcgaccgcg actccctcgg ggtgcttcgc gacgtacggc acgatgaaca
    30541 gaaggcgccg caggcgctgc gcgacatcac gcatcgaggt ctctcccgct gccctcgatc
    30601 acgtcgatcg ggccgtgtcc gttcgccgcg acgcgcgcgg cgtcgagatc cgcgaaggtt
    30661 tcgtagcgct ccgcgatcgc gtcgagctca cctgcgatgc gtgcgcgcag cttgtcaccg
    30721 cgcgtgactt gaatggcgcc tttgctcgcg aggatccgcg acaccgcgaa ctccgggttc
    30781 gtgcaatcga acgtgatcag cgtgcgatcg ccatcgtggc gcttcacggc cgttggaccg
    30841 aagtcttcgt tcgccacttc ggcggcctca gccgtcaata cgagctgcac ttcttcggcg
    30901 ggctcgctcg tgaacgtcca cggcgagcgc tgcgcgtagg ccttcacgtc gaaatcggca
    30961 ggacgctcga agtcaggcga cttcggcttg ggcgcctgca cagcctcgtg aatgcgatcg
    31021 acgcggaacg agcgaatctc gtggcgtagg tgacaccatc ccacgacgag ccacgcgccc
    31081 tcgcgataga ccatcgcgta cggatcaagg tcgcgacgcg acacgagccc gttcgtcgcc
    31141 gcttgatacg tgagcgtgac gcgcttgcga aggcgcgtcg cggattcgag cgtcgcgtag
    31201 atttcgccca gctccgggcg tgcgaccgcc gagctcgcaa gacgattctg ccgctcgggg
    31261 aagtgaacga gcaccgtcgg cgcgttgtgc gcgggacgcg gaaactccgt cggcgtgtca
    31321 ggcagctccg gcagatcgaa cgcgagcttc
//
LOCUS       DATLBZ010000633        11618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8790540, whole genome shotgun sequence.
ACCESSION   DATLBZ010000633 DATLBZ010000000
VERSION     DATLBZ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8790540"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..2405)
                     /locus_tag="VMZ53_31105"
     CDS             complement(12..2405)
                     /locus_tag="VMZ53_31105"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HUS33007.1"
                     /translation="MRRALWLPLLLACACHSCSGTDSQRHAKTPQPPPAPAVGSATAT
                     PAAMPSGGALSESTVQSYWTTPDEVEGAAKWALEEYPAAKAAFERALKATKDPDRAAR
                     LDLMIGLCAERSNDWTPAAAHLTAAYPKLPELADYIGYHAARSLYFTHDLPKSLEYAQ
                     KVARDSIVGPDAEILVGDLMTASKPDWAALVKHYRDYLDRHPKGPFRSEARFDLATSI
                     EKTEKAGADEAVKLYRQITVDDPLSSWTTKANARLAELKVSVKYTAAEHIARGKVLFD
                     EMRNPESEAAFDLALADTAISTADKCIAAYHKAQSRFKARDRKGAAPMFDTAATACQA
                     ADNKDLWIKSVYQAGRSYAFTGDHTNAIDRYKAAQGIDKTHSYSDDAMLREAEEWADL
                     NDAAKVEAVLSALPKEYPNGDNVAESMWRLGWRSWRDGKYDDAIKWWQEQIRLVPHDD
                     NYFGEGQPQYWLGRAYAAKQQVPQAIEQWKSTVRMYPAAYYSMLALNRLRETSPKDFA
                     QIVVEISSDPKDFDPKAPAFTFKPRPEWSDPRFLRAMELLRLGIGSAAEQELKKLGLT
                     APGNKTRVTDPDQIDKLWAMSFLYDKAGRYGTSHWPTRWHILDYRAQWPIGANRARWQ
                     IAYPLAYQPLLAKHATLNGVPFAMQIGIVREESAFDPLTESYANAIGLTQMIPPTAKD
                     FSKGTGIDPTRENLRDPEKNVTIGSRFLGSLFKTWKNFTLLVPPSYNAGPGAVKRWLK
                     LRGTYDADEFIEAIVDDQARNYSKRVLASFFTYTWLYEKNAVPEIPNRIPADILPKP"
     gene            complement(2486..3397)
                     /locus_tag="VMZ53_31110"
     CDS             complement(2486..3397)
                     /locus_tag="VMZ53_31110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HUS33008.1"
                     /translation="MAESEWDEETTTATELDEINEKLSSVGVRTRPCLTVLTGGASGQ
                     LFKVTKGAAVIGRAPNVELRLEDDGISRTHARLRAETDRAWIEDMGSRNGTFVNGTKI
                     TSITELREGDKIQVGRGTIIRFGMQDELDESFHENLMSSALRDGLTKLFNKRYFLDRL
                     DAELKFAQRHKTALSLLLLDLDHFKNVNDTFGHLAGDAVLATVAAALSKAVRNEDVVA
                     RYGGEEMAIILRAISIEPAAHMAERMRKVVEATPVPVDGKDVRVTISIGVAEYPTTQA
                     KQIDELIEAADKALYRAKDAGRNRVSR"
     gene            complement(3412..4269)
                     /locus_tag="VMZ53_31115"
     CDS             complement(3412..4269)
                     /locus_tag="VMZ53_31115"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HUS33009.1"
                     /translation="MSQATLAPSSAPPELATFLEEARVLVERDLETRLAVTPGADPGR
                     LGEAMHYAATGPGKRLRPALVIAACEACGGTREMALPAASAIEMLHAYTLVHDDLPAM
                     DNDDERRGRPTVHIAFGEAIAILAGDGLLTLAFGTLAELGPQAAAAVGVLARRAGVEE
                     LLAGQAIDLSAAGRVPGAVRESLADLPAIERLHAAKTGALFAAAAELGAIAAGAAPET
                     CAALGRYGLAIGIAFQHADDRDDAELVEHAAAAAERMRVLCAKAAADVRPLSSTILDG
                     FATWIASRA"
     gene            complement(4272..4535)
                     /gene="xseB"
                     /locus_tag="VMZ53_31120"
     CDS             complement(4272..4535)
                     /gene="xseB"
                     /locus_tag="VMZ53_31120"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HUS33010.1"
                     /translation="MANGPAAELGFDAILVRLREVVQKLESGELSLEQSLAIYEEGVQ
                     LARKGQHLLASAEKRVEILVSAAGGVETAPFSSASPNDDANGT"
     gene            4627..5127
                     /locus_tag="VMZ53_31125"
     CDS             4627..5127
                     /locus_tag="VMZ53_31125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HUS33011.1"
                     /translation="MGERSDFRPNDTGELKERWRTYISDRRLNTTAQREAIVEQFLRT
                     RDHVSIDELLSKVRKRQPRVGYATVYRTLKLLVDSGLAVERQFGDGQARFEVVGDHHD
                     HLICVKCGLILEFEDEEIERLQDRIAARMGGFTVLRHRHELYGLCPKAAGDAAGACPN
                     EARNKK"
     gene            5246..6298
                     /locus_tag="VMZ53_31130"
     CDS             5246..6298
                     /locus_tag="VMZ53_31130"
                     /inference="COORDINATES: protein motif:HMM:NF022932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUS33012.1"
                     /translation="MKSLPSSSVVLVAALAVGGALAGCRTDNGAGDDTPDGGSTVDAP
                     DGTGCTALTPRSQALESFVGPTGLQARMGALIDSAKTSLDIQMYLWTVKELANKVVAA
                     KQRGVTVRVILDPDELGNNNVEPIFTSGGVDWKNATTLYTYSHAKYIIVDKQQAAIMS
                     MNFNVDAMLNERNYGVIDKDPEDVADMQRIFEYDWKMANGATGLDPADLTCTRLIVSP
                     TNSKQRILEHIKSATTTLDVEVMYISEASVRTAITDAKTRGVNVRVIIEDPMDASVPV
                     FKAAGIPVKQPPSSIYLHSKLITADQVAFVGSENMSFTSLAKNREVGVLVFEPAAFAP
                     IKTQFDSDWNVSTTIP"
     gene            complement(6299..6964)
                     /locus_tag="VMZ53_31135"
     CDS             complement(6299..6964)
                     /locus_tag="VMZ53_31135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33013.1"
                     /translation="MRRAAVATMLVVALAAFAGAMFVGCKPRKTEPNGVANYRFGKTT
                     RANIFEGICQPTELTDGRKATWCFALPPLKVAKRVAEVDAYFLGQETEANSKLPEKDR
                     RPALEKMPLIEVQFKVRGCVESEAENFLRERFGPPDVKQSKGALTVWQNSFMWVGAFL
                     PSEPGRCLIHFLPMSEAAEIARIKAKAAGGAPTPEPTTPSTAPSTETPPPTATGSSAG
                     SAQ"
     gene            complement(6961..7455)
                     /locus_tag="VMZ53_31140"
     CDS             complement(6961..7455)
                     /locus_tag="VMZ53_31140"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HUS33014.1"
                     /translation="MKYSVNGGKLTVKARSAVHDTTTVWDKVSGDVDADPDAIEQAKA
                     TFTVDMTSFDAGDWLKNRKLRKDFDMDGNPTATFQLDRVSDVKRDGDKFTAKAEGKLR
                     WRGKEVPLVLSGQGTLSADRVEASAKFELDIKKLGLTAPKFFVIKMEDEVSIDVSVRG
                     AIKR"
     gene            complement(7452..8219)
                     /locus_tag="VMZ53_31145"
     CDS             complement(7452..8219)
                     /locus_tag="VMZ53_31145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HUS33015.1"
                     /translation="MKIRYAAKTDVGMKRTHNEDYFSLIEDEQLFLVADGMGGHASGE
                     VASKMAAETIGEFYQRTREDEDATWPYKMDRSLSYIENRLVCGIKLANLRIYETSNRD
                     LRYKGMGTTIVSCLVSGDKIYVGHVGDSRVYRVRDNGIQQLTRDHSLLEDYKEAKPDM
                     TEEEERNFPHKNVITRALGMRETVQVDIRSHQIKSGDVYILCSDGLSGMVPDDGIAQI
                     AANAKSLERAVAELVDAANRNGGTDNVTTLLLQCLAA"
     gene            8306..9628
                     /locus_tag="VMZ53_31150"
     CDS             8306..9628
                     /locus_tag="VMZ53_31150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HUS33016.1"
                     /translation="MHAEILTIGDELCRGEIVDTNSSGLAQRLWDLDITTRWMTSCCD
                     DENDITTALLAAVNRADLVVCSGGLGPTEDDLTVDVISRLAGQDVIIDETARARMEAR
                     FAGRIEVTPIQLRQVRVPAQSRVYVNPAGLAPCFEVDVKGVPVICLPGIPRELYAIYE
                     AHLEARFRELREARGVTERIARRIYRVFGKGESQISQACRGLVDNVAGASIHYQVKFP
                     ETLVKLVVRDVDAAAAQQRLDALDAGVRERLAGFVYGTGEENLVDKVSKRLLASGVTV
                     ATAESCTGGMIGELLTAMPGMSKVFKGGAIVYSNAEKTRQLGVRTDTLAEHGAVSEQT
                     AVEMAEGALERFGVDLAISVTGIAGPDGGTPDKPVGTVWLAAHSASGAVDPGTPSESG
                     SLHVGGGTVHLDTITKKLGWPGLRDQIRTLSAWWAFKLIDEMLERRGA"
     gene            9631..10383
                     /gene="thpR"
                     /locus_tag="VMZ53_31155"
     CDS             9631..10383
                     /gene="thpR"
                     /locus_tag="VMZ53_31155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HUS33017.1"
                     /translation="MADDKRDERSDKRDERPAGKRLFIGVRVSVQTANALAQACETLQ
                     RRAKDARPAVDLKWVAPANYHVTLKFLGFTREDAIGAVRDALDRELVGAQPVKFRTAR
                     LGAFASLDKANVLWAGVEAERGGPSSSSPLDDLYARVERAMTGIGYKAESRPFHAHVT
                     LARLRETRPLKDLVLPLSEQMFSDTRIDGVTLFESETKSSGSVYRDLHRVGFKTAGFP
                     DLATEKRQTGALELGDETQTDTDDGWPRGHGH"
     gene            10396..>11618
                     /gene="recA"
                     /locus_tag="VMZ53_31160"
     CDS             10396..>11618
                     /gene="recA"
                     /locus_tag="VMZ53_31160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HUS33018.1"
                     /translation="MASKDSGPSQAVHPPRAENVTTQNRDKAIDLALAAIDKQFGKGA
                     IMRLGKDPVEREVHVIPTGSLGLDLALGIGGLPRGRIVEIYGPESSGKTTLTLHIIAE
                     AQKRGGVCAFIDAEHALDVSYAKKLGVKSEELLVSQPDFGEQALEIADMLVRSNAVDV
                     IIIDSVAALTPKAELEGEMGDHHVGSHARLMSQALRKLTAAISKSRTMVIFINQIRMK
                     IGVMFGSPETTTGGNALKFYSSVRLDIRRVGALKKDEDVIGNRTRVKVVKNKMAPPFK
                     EVEFDILYGQGISKEGDLVDLASESNIVDKSGAWFSFNGERIGQGRENAKQFLAEHPE
                     VYNAIEAKVLTHFNVRRIGIDVAPDAAVVSDKAPESVAAKGSEKHAAPHAAASSSNAN
                     GNANGNANGKDARPAA"
BASE COUNT         1961 a         3797 c         3807 g         2053 t
ORIGIN      
        1 agtggccgct tctagggctt cggcaggatg tccgccggga tccggttcgg gatctccggg
       61 accgcgttct tctcgtagag ccacgtgtac gtgaagaacg acgcgagcac gcgcttggag
      121 tagttgcgcg cctgatcgtc gacgatcgct tcgatgaact cgtcggcgtc gtaggtgccg
      181 cgcagcttca gccagcgctt caccgcgccg ggacctgcgt tgtaggaagg cggcacgagg
      241 agcgtgaagt tcttccaggt cttgaacagc gagccgagga agcgcgagcc gatggtcacg
      301 ttcttctccg gatcgcgcag gttctcgcgc gtcggatcga tgccggtgcc tttgctgaag
      361 tctttcgccg tcggcgggat catctgcgtc aggccgatcg cgttcgcgta gctctcggtg
      421 agcgggtcga atgcgctctc ttcgcgcacg atgccgatct gcatcgcgaa cggaacgccg
      481 ttgagcgttg cgtgcttcgc gagcagcggc tggtaggcga gagggtaggc gatctgccag
      541 cgtgcgcggt tcgcgccgat cggccactgc gcgcggtagt cgaggatgtg ccagcgcgtc
      601 ggccaatggc tcgtgccata gcggccggct ttgtcgtaga ggaacgacat cgcccacagc
      661 ttgtcgatct gatcgggatc ggtgacgcgc gtcttgttgc cgggcgcggt cagtccgagc
      721 ttcttcagct cttgctctgc ggcggagccg atgccgaggc ggagtagctc catcgcgcgc
      781 aagaagcgag ggtcgctcca ctccgggcgt ggcttgaacg tgaaggcagg cgctttcgga
      841 tcgaagtcct tcgggtccga cgaaatctcg acgacgatct gcgcgaagtc cttcggcgat
      901 gtctcgcgca agcggttgag cgcgagcatc gagtagtagg ccgccgggta catgcgcacc
      961 gtcgacttcc actgctcgat cgcttgcggc acctgctgct tcgcggcgta tgcgcggccg
     1021 agccaatact gcggctggcc ttcgccgaag taattgtcgt cgtgcgggac gagccggatc
     1081 tgctcttgcc accacttgat cgcgtcgtcg tacttgccgt cgcgccacga tcgccagccg
     1141 aggcgccaca tcgattctgc gacgttgtct ccgttcgggt attccttcgg cagcgcggag
     1201 agcacggcct cgaccttcgc ggcgtcgttg aggtccgccc actcttcggc ttcgcgcagc
     1261 atcgcgtcgt cggagtagct gtgcgtcttg tcgatccctt gcgcggcttt gtagcgatcg
     1321 atcgcgttcg tgtgatcgcc ggtgaacgcg tagctgcggc ccgcctgata gacgctcttg
     1381 atccagaggt ccttgttgtc ggcggcctga cacgcggtgg cggcggtgtc gaacatcggc
     1441 gcggcgccct tgcgatcgcg tgctttgaag cggctctgcg ctttgtgata cgcggcaatg
     1501 catttgtcgg ccgtcgagat cgccgtatcg gcgagcgcga gatcgaacgc ggcctccgac
     1561 tcggggttgc gcatctcgtc gaacaggacc ttgccgcgcg cgatgtgctc tgcggccgtg
     1621 tatttcaccg agaccttgag ctccgcgagg cgcgcgttgg ccttcgtcgt ccacgacgag
     1681 agcggatcgt cgacggtaat ctggcggtag agcttcacgg cctcgtcggc accggccttc
     1741 tcggtcttct cgatcgaggt cgctagatcg aagcgcgctt ccgagcggaa cgggcctttc
     1801 ggatggcgat cgagatagtc gcggtagtgc ttcacgagcg cggcccagtc gggcttgctc
     1861 gccgtcatca gatcgccgac gaggatctcc gcatcgggac cgacgatcga atcgcgcgcg
     1921 accttctgcg cgtactcgag agacttcgga aggtcgtgcg tgaagtagag cgagcgcgct
     1981 gcgtggtagc cgatgtagtc ggcgagctcc ggcagcttcg ggtacgcggc ggtgaggtgc
     2041 gcggcggccg gcgtccagtc attcgagcgc tcggcgcaca ggccgatcat cagatcgagg
     2101 cgcgcggcgc gatcgggatc cttcgtcgcc ttgagcgcgc gctcgaacgc ggccttcgcg
     2161 gccggatact cctcgagcgc ccacttcgct gcgccctcca cctcgtccgg cgtcgtccag
     2221 tacgactgaa cggtcgattc ggacagcgcg cctcccgagg gcatcgctgc aggcgtcgcg
     2281 gtcgccgagc ccacggcggg ggcaggcggt ggctgcggtg tcttcgcatg gcgctgcgag
     2341 tccgtccccg aacaggagtg acacgcgcat gcgaggagga gtggcagcca gagagctcgg
     2401 cgcatgcctg aacgtagcaa cgtggatcgt cgccgtcgaa ttcccgtcga atcgcccacc
     2461 aagagtgtga ccaacgctgt gatcgtcagc gcgaaacgcg gttgcggccc gcgtccttgg
     2521 ctcggtacag cgccttgtcg gcggcctcga tgagctcgtc gatctgcttt gcctgggtcg
     2581 tcggatactc ggcgacgccg atcgagatcg tgaccctgac gtccttgccg tcgaccggca
     2641 ccggcgtcgc ttcgacgacc ttgcgcatgc gctcggccat gtgcgccgcg ggctcgatgc
     2701 tgatcgcgcg caggatgatc gccatctctt cgccgccgta gcgcgcgacg acgtcctcgt
     2761 tgcggaccgc cttggacagc gccgccgcga cggtcgcgag caccgcgtct ccggccaggt
     2821 gaccgaacgt gtcgttgacg ttcttgaagt gatcgagatc gagcagcagg agcgacagcg
     2881 ccgtcttgtg ccgctgcgcg aacttcagct cggcatcgag tcgatcgagg aagtagcgct
     2941 tgttgaagag cttcgtcagg ccgtcgcgca gggccgacga catgaggttt tcgtggaacg
     3001 actcgtcgag ctcgtcctgc atgccgaacc ggatgatcgt gccgcggccg acttggatct
     3061 tgtcgccttc gcgcagctcc gtgatcgacg tgatcttggt gccgttgacg aacgtgccgt
     3121 tgcggctgcc catgtcctcg atccaggcgc gatcggtctc cgcacggagg cgagcatgcg
     3181 tgcgcgagat gccgtcgtcc tcgaggcgca gctcgacgtt cggtgcacga ccgatgacgg
     3241 ccgcgccttt cgtgaccttg aagagctgac ccgatgcgcc gcccgtcaga acggtgagac
     3301 acggacgcgt gcgaacgccg acggacgaga gcttctcgtt gatctcgtcg agctccgtcg
     3361 cggtcgtggt ctcctcgtcc cactcgcttt cagccaccga tccattctgc atcaggcgcg
     3421 agatgcgatc caggtggcga atccgtcgag aatcgttgag ctcagtggtc ggacatctgc
     3481 cgccgctttg gcgcagagca cgcgcatgcg ctcggcggct gccgcagcgt gctcgacgag
     3541 ctcggcatcg tcgcgatcgt cggcgtgctg aaaggcgata ccaatcgcaa ggccgtagcg
     3601 accgagtgcc gcgcaggtct cgggagccgc gcctgctgca atcgcgccca gctcggcggc
     3661 cgccgcgaac agtgcgccgg tcttcgcagc gtgaagtcgc tcgatcgcgg gaaggtctgc
     3721 gagcgattcg cgtaccgccc cagggacgcg tcccgccgcc gacaagtcga tcgcctgtcc
     3781 tgcgagcagc tcttcgacac ccgcgcgccg cgcgagcacc ccgaccgccg ccgctgcctg
     3841 tggtccgagc tcggcgagcg taccgaacgc aagcgtcagc aggccatcgc cggccaagat
     3901 cgcgatcgcc tcgccgaatg cgatgtgcac cgtcgggcga ccgcgccgtt cgtcgtcgtt
     3961 gtccattgcg ggcaagtcgt cgtggacgag cgtgtacgcg tgcagcatct cgatcgccga
     4021 cgctgcaggc agtgccatct cgcgcgtccc tccacacgcc tcgcacgcgg cgatcaccaa
     4081 cgccggccgc agccgcttgc caggccccgt cgctgcgtag tgcatcgcct cgccgagccg
     4141 tccgggatcc gcgccgggcg tcaccgccag ccgcgtctcc aagtcgcgtt cgacgagcac
     4201 gcgcgcttcc tcgaggaacg tcgccagctc gggcggcgcg ctcgacggag ccaacgtcgc
     4261 ttgcgacacc gctaggttcc gttcgcgtcg tcgttcggtg atgccgacga aaacggcgcc
     4321 gtctcgacgc cacccgcggc gctcaccaag atctcgacac gcttctcggc ggacgcgagc
     4381 aggtgctgac ccttgcgagc gagctgcacg ccttcttcgt agatggccaa gctctgctcg
     4441 agcgacagct cgcccgactc gagcttctgc acgacctcgc gcaagcgcac gaggatcgcg
     4501 tcgaacccga gctccgctgc tggtccattc gccacggccg ccgcaccata gcgaactgaa
     4561 actgaaaagt catcccgtcg agatcgcttg acgaaccgcc cacttgcggg gaagctgaaa
     4621 cactcaatgg gggagcgctc ggactttcgg cccaacgata cgggcgagct caaggagcgt
     4681 tggcgcacct atatctccga ccgccgcctc aacacgaccg cgcaacgcga agccatcgtc
     4741 gagcaattcc tgcgcacgcg cgaccacgtc tcgatcgacg agctcctctc gaaggttcgc
     4801 aagcgccaac ctcgcgtcgg ctacgcgacc gtctaccgca ccctcaagct cctcgtcgac
     4861 agcggcctcg cggtcgagcg ccagttcggc gacggccaag cgcgcttcga ggtcgtcggc
     4921 gatcaccacg accatctcat ctgcgtgaag tgcggcctca tcctcgagtt cgaagacgaa
     4981 gagatcgagc gcctgcaaga tcgcatcgcc gcacggatgg gcggcttcac tgtccttcgc
     5041 caccgccacg agctctacgg cctctgtccc aaagccgcag gtgacgccgc gggcgcctgt
     5101 ccgaacgaag cccgcaacaa gaagtaaccc gccgaaagcg ctcgctgcgc acgcgaggtt
     5161 gcagagcgtg agatcacgag cgagggaccg gccaccgttt tgtgcttggt gatttcgctt
     5221 cgccgcgggc atagtccgcg accgcatgaa gtcgttgccg tcgtcgtcgg ttgttctcgt
     5281 cgcggcgctc gccgtcggcg gcgcgctggc cggctgccga accgacaatg gcgcgggcga
     5341 cgacacgccg gatggcggct cgacggtcga tgccccagac ggtacgggct gcactgcgct
     5401 cacgccgcgg tcgcaagcgc tcgagtcgtt cgtcggcccg acgggactgc aggcgcgcat
     5461 gggcgccctc atcgatagcg cgaagacctc gctcgatatc cagatgtatc tctggacggt
     5521 caaggagctc gcgaacaagg tcgtcgccgc caagcagcgc ggcgtgaccg tgcgcgtgat
     5581 cctcgacccc gacgagctcg gcaacaacaa cgtcgagccg atcttcacgt cgggcggcgt
     5641 cgactggaag aacgcgacga cgctctacac gtactcgcac gccaagtaca tcatcgtcga
     5701 caagcagcag gccgcgatca tgtcgatgaa cttcaacgtc gacgcgatgc tcaacgagcg
     5761 caactacggc gtcatcgaca aggatcccga agacgtcgcc gatatgcagc gcatcttcga
     5821 gtacgactgg aagatggcga acggcgcgac cggcctcgac ccggcggacc tcacgtgcac
     5881 gcgcctgatc gtctcaccga cgaacagcaa gcagcgcatt ctcgagcaca tcaagagcgc
     5941 gacgacgacc ctcgacgtcg aggtcatgta catctccgaa gcgtccgtgc gcaccgcgat
     6001 caccgatgcg aagacgcgtg gcgtcaacgt gcgcgtcatc atcgaagatc cgatggacgc
     6061 ctccgtgccg gtcttcaaag cggcgggcat tcccgtcaag cagccgccgt cgtcgatcta
     6121 tctgcactcc aagctcatca ccgcggacca agtcgcgttc gtcggcagcg agaacatgag
     6181 cttcacgtcg ctcgcgaaga atcgcgaagt cggcgtcctc gtcttcgagc cggccgcgtt
     6241 cgcgccgatc aagacgcagt tcgattccga ctggaacgtc tcgacgacga tcccgtagct
     6301 actgcgcgct gccggcggag ctgcccgtcg cggtcggcgg aggcgtctcg gtactcggtg
     6361 cggtgctcgg tgtcgtcggc tcgggcgttg gcgcgccgcc cgcggccttg gccttgatgc
     6421 gcgcgatctc ggcagcctcg gacatcggca agaaatgaat caggcagcgg ccgggctcgc
     6481 tcgggagaaa cgcgccgacc cacatgaacg agttctgcca gacggtcagc gcgcccttgg
     6541 attgcttgac gtccggcggg ccaaaccgct cgcgcaggaa gttctccgct tcactctcga
     6601 cgcagccgcg cactttgaac tgcacctcga tcagcggcat cttctcgagc gccggcctgc
     6661 gatctttttc gggcagcttc gagtttgctt cggtttcctg gccgaggaag tacgcgtcga
     6721 cctccgccac gcgcttggcc accttgagcg gcggtagcgc gaaacaccac gtggccttgc
     6781 ggccgtcggt gagctcggtc ggttggcaga taccttcgaa gatgttcgcg cgcgtcgtct
     6841 tgccgaaccg atagttcgcg acgccgttcg gctcggtctt gcgaggtttg cagccgacaa
     6901 acatcgcgcc cgcgaaggca gcaagtgcga cgacgagcat cgtagcgacg gcagcgcgcc
     6961 tcatcgcttg atggctccac gcacgctgac gtcgatcgac acttcgtcct ccatcttgat
     7021 cacgaagaac ttcggcgcgg tgaggccgag cttcttgatg tcgagctcga acttcgcgct
     7081 cgcctcgacg cgatcggcgg acagcgtgcc ttgcccactc aacacgagcg gcacttcctt
     7141 gccgcgccac ctgagcttgc cttcggcttt cgcggtgaac ttgtcgccat ctcgctttac
     7201 gtcagacacg cggtcgagct gaaacgttgc ggtcgggttg ccgtccatgt cgaagtcctt
     7261 gcggagcttt cgattcttca gccagtcacc cgcgtcgaac gacgtcatgt cgacggtgaa
     7321 cgtcgccttc gcctgctcga tcgcgtcggg atctgcgtcg acgtcgcccg agactttgtc
     7381 ccagaccgtc gtcgtgtcgt gcacggcaga ccgcgctttg acggtgagct tgccgccgtt
     7441 gacggagtac ttcatgccgc cagacactgc agcagcagcg tcgtgacgtt gtccgtgccg
     7501 ccgttgcggt tcgctgcatc gacgagctcg gcgactgcgc gctcgaggct ctttgcgttc
     7561 gcggcgatct gcgcgatgcc gtcatcggga accatgcccg agagaccgtc cgagcacagg
     7621 atgtagacgt cgccgctctt gatctggtgg ctgcggatat cgacctggac ggtctcgcgc
     7681 atgccgagcg cgcgcgtgat gacgttcttg tgggggaagt tgcgctcttc ctcctcggtc
     7741 atgtccggct tcgcttcctt gtagtcctcg aggagcgagt ggtcgcgcgt gagctgctgg
     7801 atgccgttgt cgcggactcg atagacgcga gagtcgccga cgtggccgac gtagatcttg
     7861 tcgcccgaca cgaggcacga aacgatcgtc gtacccatgc ccttgtagcg aaggtcgcgg
     7921 ttgctggtct cgtagatgcg gaggttcgcg agcttgatgc cgcagaccag ccggttctcg
     7981 atataggaga gcgagcggtc catcttgtag ggccacgtcg cgtcttcgtc ctcacgcgtc
     8041 cgctgataga actcgccgat cgtctcggcg gccatcttgg acgcgacctc gccggatgcg
     8101 tgaccaccca tgccgtcggc cactaggaag agctgttcgt cctcgatcaa cgagaagtaa
     8161 tcctcgttgt gcgtccgctt catcccgacg tccgtctttg ccgcgtagcg gatcttcatg
     8221 gtgaccgcag tccgtcgaaa tcataggcaa ccgattggag tcgcgtcaac cgaaccgccc
     8281 gtgttccgag tagagtgcac agcgcatgca cgcggagatc ttgacgatcg gagacgagct
     8341 gtgccgcggc gagatcgtgg ataccaactc ctcgggcctt gcgcagcgct tgtgggacct
     8401 cgacatcacc acccggtgga tgacgagttg ctgcgacgac gaaaacgata tcacgaccgc
     8461 gctactcgcg gcagtgaacc gcgcggacct cgtagtctgt tcaggcggcc tgggcccgac
     8521 cgaggacgat ttgacggtcg acgtcatttc gaggctcgcc gggcaggacg tcatcatcga
     8581 cgagacggct cgcgcgcgca tggaagcgcg gttcgcggga cgtatagaag tgacgccgat
     8641 ccagctgcgg caggtgcgcg ttcccgccca gtcacgcgtc tatgtgaatc ccgccggcct
     8701 cgcgccgtgc ttcgaggtcg acgtcaaggg cgtgcccgtg atctgtctgc ccggcatccc
     8761 gcgcgagctc tacgcgatct atgaagccca cctcgaagcg cggtttcgcg agctccgcga
     8821 ggcgcgcggc gtcaccgagc ggatcgcgcg gcggatctat cgagtgttcg gcaaaggcga
     8881 gtcgcagatc tcgcaggcgt gccgcggtct cgtcgacaac gtcgccggcg cgtcgatcca
     8941 ctatcaggtg aagtttcccg agacgctcgt caagctcgtc gtgcgcgatg tcgacgcggc
     9001 agcggcgcag caacggctcg acgcgctcga cgccggcgta cgcgaacggc tcgcgggctt
     9061 cgtctacggc accggcgagg agaacctcgt cgacaaggtc agcaagcgtc tgctcgcgag
     9121 cggagtcacc gtcgcgaccg ccgaatcgtg cacgggcggc atgatcggcg agctgctcac
     9181 ggcgatgccc ggcatgtcga aggtgttcaa gggcggcgcg atcgtttact ccaatgcaga
     9241 gaagacgcgg cagctcggtg tccgcacgga cacgctcgca gagcatggtg ccgtcagcga
     9301 gcagaccgcg gtcgagatgg ccgagggtgc gctcgagcgg ttcggcgtcg acctcgcgat
     9361 cagcgtgacg ggcatcgcgg gtcccgacgg cggcacaccc gacaagcccg tcggcacggt
     9421 gtggctcgcg gcgcatagcg caagtggcgc ggtcgatccc gggaccccga gtgaatcggg
     9481 atccctccat gttgggggcg gcaccgtgca cctcgacacg atcacgaaga agctcggctg
     9541 gcccggattg cgcgatcaga tccggacgct gagcgcgtgg tgggccttca agctcatcga
     9601 cgagatgctc gagcgacgag gagcctgacg atggccgacg ataagcgcga cgagcgaagc
     9661 gacaagcgcg acgagcgacc ggcgggaaag cggctgttca tcggggtgcg cgtcagcgtg
     9721 cagacggcga acgccttggc gcaggcgtgc gaaacgctcc agcgtcgagc gaaggacgcg
     9781 cggcctgcgg ttgatctcaa gtgggtcgcg ccggcgaact atcacgtgac gctgaagttc
     9841 ctcggcttca cccgcgagga cgcgatcggc gccgtgcgtg atgccctcga tcgcgagctc
     9901 gtgggcgcgc agcccgtgaa gtttcgaacc gcacgcctcg gtgcgtttgc gtcgctggac
     9961 aaggcgaatg tgctgtgggc aggcgtcgaa gcggagcggg gcggaccgag cagctccagc
    10021 ccactcgacg acctctacgc gcgcgtcgag agagccatga ccgggatcgg ctacaaggcc
    10081 gagtcgcgac cttttcatgc gcatgtgacg ctcgcgaggc tccgcgaaac tagacctctc
    10141 aaggacctcg tgttacccct gtccgaacaa atgttcagtg acacgcgcat cgatggggtg
    10201 acgctgttcg aaagcgaaac gaaatcgtcc ggttccgtgt atcgagatct ccatcgagtc
    10261 ggcttcaaaa cggccggttt tccggatttg gcgaccgaaa aacgtcagac cggcgcgcta
    10321 gagctggggg acgaaacgca gaccgatacg gatgacggct ggccgcgagg gcacggccac
    10381 taaaggagat ttgcgatggc ttcgaaagac tcgggaccca gtcaggcagt tcaccctccg
    10441 cgcgcggaga atgtgaccac gcagaaccgc gacaaggcga tcgacctcgc gcttgccgcg
    10501 atcgacaagc agttcggcaa gggcgcgatc atgcgcctgg gcaaggatcc cgtggagcgc
    10561 gaggtccacg tcatcccgac gggctcgctc ggcctcgatc tcgctcttgg catcggcggc
    10621 ttgccgcgcg gtcgcatcgt cgagatctac ggcccggagt catccggtaa gacgacgttg
    10681 accctgcaca tcattgccga ggcgcagaag cgcggcggcg tgtgcgcgtt catcgatgcc
    10741 gagcacgcgc tcgacgtcag ctacgcgaag aagctcggcg tgaagagcga agagctcctc
    10801 gtcagccagc cggacttcgg cgagcaggcg ctcgagatcg ccgacatgct cgtccgctcg
    10861 aacgcggtcg acgtcatcat catcgactcc gtcgcggcgc tcacgccgaa ggccgagctc
    10921 gagggcgaga tgggcgatca ccacgtcggc tcgcacgctc gcctcatgag ccaggcgctg
    10981 cgcaagctca cggccgcgat ctcgaagtcg cgcacgatgg tcatcttcat caaccagatc
    11041 cgcatgaaga tcggcgtcat gttcggctcg cccgagacga cgacgggcgg taacgcgctc
    11101 aagttctact cgtcggtgcg tctcgacatc cgtcgcgtcg gtgccctcaa gaaggacgaa
    11161 gacgtcatcg gcaaccgcac ccgcgtgaag gtcgtgaaga acaagatggc gccgccgttc
    11221 aaggaggtcg agttcgacat cctctacggc caaggcatct cgaaggaagg cgacctcgtc
    11281 gacctcgcgt cggagtcgaa catcgtcgac aagagcggcg cgtggttctc gttcaacggc
    11341 gagcgcatcg gtcagggccg cgagaatgcg aagcagttcc tcgccgagca ccccgaggtc
    11401 tacaacgcga tcgaggcgaa ggtgctcacg cacttcaacg tccgccgcat cggcatcgac
    11461 gtcgcgcccg acgctgcagt cgtgagcgac aaggcgcccg agagcgttgc cgcgaagggc
    11521 agcgaaaagc acgctgcgcc gcacgccgct gcgtcgagca gcaacgcgaa cggcaacgcc
    11581 aacggcaacg ccaacggcaa ggacgcgcgt ccggccgc
//
LOCUS       DATLBZ010000634         5700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10169225, whole genome shotgun sequence.
ACCESSION   DATLBZ010000634 DATLBZ010000000
VERSION     DATLBZ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10169225"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VMZ53_31165"
     CDS             <1..388
                     /locus_tag="VMZ53_31165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33019.1"
                     /translation="TRVGAEAGIDAELSSLTSWLTLHTQASLAATRIMTSGTYCVDPM
                     GIAKDCDEGMPVNNTMISGKQTGVYPSGTATLSLAAERREGWFRTAQLALLGSVGTMP
                     LIKDGAKQGSGTYTGLGLTLTLGVGD"
     gene            401..1768
                     /locus_tag="VMZ53_31170"
     CDS             401..1768
                     /locus_tag="VMZ53_31170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS33020.1"
                     /translation="MNLAQDRTLRTFTLCVLYVAQGIPWGFMATTLPGYLKQHGVSAE
                     IVGAMLSFTTLPYAFKWVWGPIIDTFTLPRFGRRRPWIVFSQAMMALTVIVLVTFDLG
                     TEIKLLTWTILIHTVFNSLQDVSVDALATDLLDDSERGRANGLMYGSKYLGGLIGGVF
                     VAKLIRYTNLDTALIAQTAVLGAIMLVPLLVKERDANESASLPEARVRSAPAQLSFNQ
                     TIAGLGEAFSLRSTVVCALLMLCANFAIGCVSANGYELFIGRLGWSYDQLAVITGGWG
                     LLVGAVCAASTGFLVDKLGRRTVAAIACCALAGGWITFSQLEPYWHSHTLIYIAGFWE
                     GAWQSIVSVALIALCMDLSSPKVAGSQFAAYMALSNFSTTLGYQYAARITKALDFPHM
                     YIAMGLTQLALVLLLLPIDPKELRRSIDSPELAKRGNVGLIAFLVLVAFLIVMTIKKT
                     IDTVG"
     gene            1783..2034
                     /locus_tag="VMZ53_31175"
     CDS             1783..2034
                     /locus_tag="VMZ53_31175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33021.1"
                     /translation="MRFAVVLALVAGCTQPRSKTCTDICSRESDCITSTNSTIPFDEK
                     ECVVACEMLRNDQQNLAKVEQHKECVLSKPSCSDVLECP"
     gene            complement(2031..2309)
                     /locus_tag="VMZ53_31180"
     CDS             complement(2031..2309)
                     /locus_tag="VMZ53_31180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33022.1"
                     /translation="MRYLLLAALLVPSIAFADTAPDVSKMKSDDCARARKANKTCVLD
                     MGGETLEGEKVTATGTAVNARQFAVLGSLIRIRKDFITEILKSAENVD"
     gene            complement(2355..2798)
                     /locus_tag="VMZ53_31185"
     CDS             complement(2355..2798)
                     /locus_tag="VMZ53_31185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33023.1"
                     /translation="MGSEDRYRLAAVRDVRARTEKTRKGELANAVGDAHETESRLAAA
                     ATRTQAAKTAWLAARATRDTLLASGATSTHLARADHFIERRRRELQRAIAEEARCETA
                     HLERQGKVDAARLVLVRARAEREIIERHFERWRSERKKLIERRED"
     gene            2822..4492
                     /locus_tag="VMZ53_31190"
     CDS             2822..4492
                     /locus_tag="VMZ53_31190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33024.1"
                     /translation="MLQAGDAPIDRYVVLEHLSEGGMGAIYVGKKMGAGGFEMEVVLK
                     QLLPEFTQQEEFIDLFLREAKLSATLDHANIVHTIDLVTAGGEYFIVMEYLPGGDLRT
                     LLKKAKRRGRRFSPAAAIYICREIASALSYAHSKRDFDGQPLKLIHRDVSPSNVLLSY
                     QGEVKLTDFGIAKAATHTSLFYKVKGKIGYMSPEQAKSESLDHRSDLYSLAVCFYEIL
                     TGERLFVHAGLTTSADEIYSQPVPQVTRKVPGLPADLDAIMAKALSIDPAKRYQTAGE
                     LQEALMRCAHRNGLLMSAPELATELREACGPTETWRADEEDDEASGAPRAGTEVYDAV
                     DDEDDELDVGPMSIHSLAAKANAKGPARGQPLSSAKSPPLNVKSPPPGLPPIRRKTEV
                     GKFEGVELTSIINMMDLDEQGSKPLVDLDSGPRSIPPAPQPRARVDSDPSLSQFVNSL
                     PGHGAPPPPPMAPPRQSGGAPIPHTMGGFGAVPPPAHFDQSDVHPTMPRPFAPPQVAE
                     RTDRRLAVGRRQKPKSFIQPWMVIAAIVLLAIIIALVVAMSGPDVPAK"
     gene            4570..>5700
                     /locus_tag="VMZ53_31195"
     CDS             4570..>5700
                     /locus_tag="VMZ53_31195"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HUS33025.1"
                     /translation="MTKDRSNVTALLDDAPSYSRQNAGGIFVVIKGPDRGEAVRLNTQ
                     EVAVSFGSGPQCSLVLTDKTVSRKHLEASLVGDEVVMTDCGSTNGTFIQGSRFERISI
                     GFGAEVKLGRTVIKFLPDEEIVEPEPSAQARFGSIVGGDTKMRQMFTLLEDVSATNAT
                     VLIEGETGTGKELIAEEVHNHSPRKDGPFVVFDCGSVPRELIESMLFGHVKGSFTGAI
                     TDRRGAFAEAHGGTIFLDEIGEMALDLQPSLLRVLDKRAVRKVGSNTYEKIDVRVVAA
                     TNRDLRAEVAKKVFREDLYYRLAVIRVGVPPLRERGSDIPLLIQHFVNTFGQGLQVTP
                     EDMARLVRHSWPGNVRELRNVIERACLLARGQSINLEDALVSE"
BASE COUNT          974 a         1850 c         1846 g         1030 t
ORIGIN      
        1 cacgcgcgtc ggtgcagaag cgggcatcga tgcggagctc tcgtcgctca cgtcgtggct
       61 cacgctccac acgcaagcgt cactcgcagc gacacgcatc atgacgagcg gcacctactg
      121 cgtcgatccg atgggcatcg caaaggattg cgacgagggg atgccggtga acaacacgat
      181 gatcagcggc aagcagacag gcgtctatcc cagcggcacc gccacactct cgctcgcggc
      241 cgaacgtcgc gaaggttggt ttcgcacggc gcagctcgcg ctgctcggct cggtcggcac
      301 gatgccgctc atcaaggacg gcgcgaagca gggcagcggc acgtacacgg gcctcggcct
      361 gacgctgacg ctcggcgttg gcgactgata ctgttgcgac gtgaacctcg cgcaggatcg
      421 cacgctacgt acgttcacgc tgtgcgtgct ctacgtcgcg cagggaatcc cgtggggctt
      481 catggcgacg acgctgcctg gctatctcaa gcagcacggc gtctcggccg agatcgtcgg
      541 cgcgatgctg tccttcacga cgcttccgta cgcgttcaag tgggtgtggg gaccgatcat
      601 cgacacgttt acgctcccgc gctttggccg gcgacgaccg tggatcgtgt tctcgcaggc
      661 gatgatggcg ctcacggtca tcgtgctcgt cacgttcgat ctcggcaccg agatcaagct
      721 gctcacgtgg acgatcctga tccacaccgt cttcaactcg ctgcaagatg tgtccgtcga
      781 tgcgctcgcg acggatcttc tcgacgacag cgagcgcggc cgcgcgaacg gcctcatgta
      841 cggttcgaaa tacctaggcg gcctcatcgg cggcgtgttc gtcgccaagc tgatccgcta
      901 cacgaacctc gatacagccc tcatcgcgca gaccgccgtg ctcggcgcga tcatgctcgt
      961 gccgctgctc gtcaaagagc gcgacgcgaa cgagagcgct tcgcttcccg aggcccgcgt
     1021 ccgctcggcc cctgcgcagc tgtcgttcaa ccaaacgatt gccggactcg gcgaagcatt
     1081 ctcgctccgc tcgacggtcg tctgcgcgtt gttgatgttg tgtgcaaact tcgcgatcgg
     1141 ttgcgtgtcg gcgaacggct acgagctgtt catcggccgc ctcggttggt cctacgacca
     1201 gctcgcggtc atcaccggcg gttggggctt actcgtcggc gcggtgtgcg cagcctcgac
     1261 cggatttctc gtcgacaagc tcggccgtcg aaccgtcgcg gcgatcgcgt gctgcgccct
     1321 cgccggtggc tggatcacgt tctcgcagct cgagccgtac tggcacagcc acacgctcat
     1381 ctatatcgcc ggtttctggg aaggtgcgtg gcagtcgatc gtgtccgtgg cactcatcgc
     1441 gctgtgcatg gatctttcgt cgccgaaggt cgccggctcg cagttcgccg cgtacatggc
     1501 gctgtcgaat ttctcgacga cgcttggcta tcaatacgct gcgcggatca cgaaggcgct
     1561 cgacttcccg cacatgtaca tcgcgatggg gctcacgcag ctcgcactgg tgctgctgct
     1621 tcttccgatc gatcccaagg agctgcgccg ctcgatcgac tcacccgagc tggcgaaacg
     1681 cggcaacgtc ggcctcatcg cattcctggt gctcgtcgcg ttcctcatcg tgatgaccat
     1741 caagaagacg atcgacaccg tcggttgagc tagactctcg ccatgcggtt cgcggtcgtc
     1801 ctcgctctcg ttgctggctg cacgcagccg cgcagcaaga cgtgcacgga catctgctcg
     1861 cgcgagagcg actgcatcac gtcgacgaac agcacgattc cattcgacga gaaggaatgc
     1921 gtcgtcgcgt gcgagatgct gcgcaacgat cagcagaacc tcgcgaaggt cgagcagcac
     1981 aaggaatgcg tgctcagcaa gccgtcgtgc agcgacgtcc tcgagtgtcc ttagtcgacg
     2041 ttctcggctg acttcaggat ctcggtgatg aagtctttgc gaatgcgaat gaggctgccg
     2101 agtaccgcga actgacgcgc gttgacggcg gtgccggtcg cggtgacttt ctcgccttcg
     2161 agcgtctcgc cgcccatgtc gagcacgcag gtcttgttcg ccttgcgagc gcgggcgcag
     2221 tcgtcgctct tcatcttcga cacgtcgggg gcggtgtcgg caaacgcgat cgagggcacg
     2281 aggagggcag cgagcaggag gtagcgcatg gcacagagac acgacgcgcg ccgtcgagtt
     2341 gcacctcgta agtctcagtc ctcgcgacgc tcgatcagct tcttgcgctc gctgcgccag
     2401 cgttcgaagt ggcgctcgat gatctcgcgt tcggcgcgcg cgcggacgag cacgagccgg
     2461 gcggcgtcta ctttgccctg gcgttcgagg tgggccgtct cgcaccgcgc ttcttcggcg
     2521 atggcacgct gaagctcccg gcggcgacgt tcgatgaagt ggtcggcgcg ggcgaggtgt
     2581 gtgctcgtcg ctccggaggc gagcagggta tcgcgcgtag cgcgagcggc gagccaggcg
     2641 gttttcgcag cttgggtgcg tgtcgcagcc gcggcgagcc ggctctcggt ttcatgcgcg
     2701 tcgccgaccg cgttcgcgag ctcgcctttg cgcgttttct cggtgcgcgc ccgtacgtcg
     2761 cgtaccgcag cgagtcggta ccggtcctcg ctgcccatag tggaatgtta cactgctgct
     2821 cgtgcttcag gccggggacg cgcctatcga ccggtatgtc gtgctcgagc acctcagcga
     2881 aggtggcatg ggcgcgatct acgtcggcaa gaagatgggc gccggcggct tcgagatgga
     2941 ggtcgtgctc aagcagctgc tgcccgagtt cacgcagcag gaagagttca tcgatctctt
     3001 tctgcgcgag gcgaagctgt cggccacgct cgatcacgcg aacatcgtcc acacgatcga
     3061 tctggtgacg gcgggcggcg agtacttcat cgtcatggag tacctgccgg gcggcgatct
     3121 gcggacgctg ctcaagaagg cgaagcggcg tggcaggcgc ttctcgccag cggcagcgat
     3181 ctacatctgt cgcgagatcg cgagcgcgct gtcgtacgcg cacagcaagc gcgacttcga
     3241 cggccagccg ttgaaactga tccaccgcga cgtgtcgccg tcgaacgtgt tgctctcgta
     3301 tcagggcgaa gtgaagctga ccgacttcgg catcgcgaag gccgcgacgc acacgtcgct
     3361 gttctacaag gtgaagggga agatcgggta catgtcgccg gagcaggcga agagcgagtc
     3421 gctcgatcac cgcagcgatc tctattcgct ggccgtttgc ttctacgaga tcctcacggg
     3481 cgagcgtctg ttcgtgcacg ccggcctgac gacgtccgcc gacgaaatct actcgcagcc
     3541 cgtgccgcag gtgacgcgca aggttccggg gctgccggcc gatctcgacg cgatcatggc
     3601 gaaggcgctc tcgatcgatc cggcgaagcg ctatcagacg gccggcgagc tgcaagaagc
     3661 gctcatgcgt tgcgcgcatc gcaacggtct cttgatgtcg gcgcccgagc tcgcgacaga
     3721 gcttcgcgag gcctgcggtc cgacggagac ctggcgcgcc gacgaagagg acgacgaagc
     3781 atccggcgcg ccgcgcgcgg gtacggaggt ctacgacgcg gtcgatgatg aagacgacga
     3841 gctcgatgtc gggccgatgt cgatccactc gctcgccgcg aaggccaatg cgaagggacc
     3901 cgcgcgaggc caaccgctca gcagcgcgaa gagcccaccg ctcaatgtga agtcgccgcc
     3961 gccgggcctg ccaccgattc ggcgcaagac cgaagtcggc aagttcgagg gcgtcgagct
     4021 gacgtcgatc atcaacatga tggatctcga cgagcagggc tcgaaaccgc tcgtcgatct
     4081 ggattcgggc ccgcgctcga tcccaccggc gccgcagccg cgcgctcgcg tcgattcgga
     4141 tccctcgctg tcgcagttcg tgaattcact gcccgggcac ggcgctccgc caccgccgcc
     4201 gatggcgccg ccgcgacagt caggcggtgc accgattcct catacgatgg gcggcttcgg
     4261 tgcggttccg ccgcccgcgc acttcgatca atcggacgtt catccgacga tgccgcgtcc
     4321 gttcgcgccg ccgcaggtcg ccgaacgcac cgatcgccgg ctcgcggtcg gccgtcgaca
     4381 aaagccgaag tcgttcatcc aaccgtggat ggtgattgcg gcgatcgtgc tgctcgcgat
     4441 catcatcgcg ctcgtcgttg cgatgtcggg tccggacgtc cccgcaaagt agcgaggatc
     4501 gttaccggat ctagaggggt gggggtatag acaccagggg cccagcggtc taccatcctc
     4561 tggctagacg tgacgaagga ccgctccaat gtgaccgcgc tcctggacga cgctccgtcc
     4621 tattcgcggc aaaacgcggg cggcattttc gtcgtgatca aaggacctga tcgcggcgag
     4681 gccgtccgac tgaacaccca agaggtcgct gtctcgttcg gatcgggacc gcaatgcagc
     4741 ctcgtgctga ccgacaagac ggtgtcgcgt aagcatctcg aagcatcgct cgtcggcgac
     4801 gaagtcgtga tgaccgactg cggctcgacg aacggcacgt tcatccaagg ctcacgcttc
     4861 gagcgcatct cgatcggctt tggcgccgaa gtgaagctcg gccgcacggt catcaagttc
     4921 ctgccggacg aagaaatcgt cgagccggag ccgagtgccc aagcgcgctt cggatcgatc
     4981 gtcggcggcg atacgaagat gcggcagatg ttcacgctgc tcgaggacgt ctccgcgacg
     5041 aacgccaccg tcctgatcga aggcgagacc ggcacgggca aagagctcat cgccgaagag
     5101 gtccacaatc actcgcctcg caaagatggc ccgttcgtcg tgttcgactg cggctccgtc
     5161 ccgcgcgagc tgatcgagtc gatgttgttc ggtcacgtga agggctcgtt cacgggcgcc
     5221 atcaccgatc gccgcggcgc atttgccgaa gcacacggcg gcacgatctt cctcgacgag
     5281 atcggcgaga tggcgctcga cctccagccg tcgctcctgc gcgtgctcga caagcgcgcc
     5341 gtccgcaagg tcggctcgaa cacgtacgag aagatcgacg tccgcgtcgt cgcggcaacg
     5401 aaccgcgatc tgcgtgcgga agtcgcgaag aaggtgttcc gcgaagacct ctactaccgc
     5461 ctcgcggtca tccgcgtcgg cgtgccgccg ctgcgcgagc gcggcagcga catcccgctg
     5521 ctcatccagc acttcgtgaa cacgttcggc caaggtctcc aggtcacgcc tgaagacatg
     5581 gcgcgtctcg tgcgccacag ctggccaggc aacgtccgcg agctgcgcaa cgtgatcgag
     5641 cgtgcgtgcc tcctcgcgcg cggccagtcg atcaatctcg aggacgcact cgtcagcgaa
//
LOCUS       DATLBZ010000635         4259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7925651, whole genome shotgun sequence.
ACCESSION   DATLBZ010000635 DATLBZ010000000
VERSION     DATLBZ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7925651"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VMZ53_31200"
     CDS             <1..640
                     /locus_tag="VMZ53_31200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33026.1"
                     /translation="SHVDYTNCPGQRPAGAPLEGSWIDVPNSHVRILPVCSMHPAQVG
                     PYHFGEGAVAQDQCALPTAASGWLEGQTLALVIDFLDEDRKPAFRVFYQDAPTDAPIG
                     HVPAVMLADKAVDVSLLCVGSSDSVEDHPTKIVANTKPRFALSGHWEDFLQPVGAPPK
                     PIPLLDLQGYVTRAEAALPGPADAPLVVDGEPRTTRHVLVDPGARFVVPPAP"
     gene            656..2488
                     /locus_tag="VMZ53_31205"
     CDS             656..2488
                     /locus_tag="VMZ53_31205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33027.1"
                     /translation="MSRRSDDEGDTQVSSPKLAIADENIATQTSSSKLPIDRIAHAAA
                     AVQRPSVHDLETSIAPDVRTNPRSRPPTKTPSTAGGSTTVGSPLEALERDEILRTRWF
                     CLIGLAVGLAGGASVAFLPGDPTVSLILLTAVAVAICAVVFLWMRTRDPIAFRKPSTG
                     LGWFIPAACVTCAIPFYGAFSPVAVVLVLGVYFTGLGKSTKLAMAVYAVCAGMQAFIG
                     ILVIAGMRDTGIVHVEFLDTETKLVIQALVQVVLLATLITARMSRRTALAAVGELERV
                     VRQVAHREALLLEAREELERALRQGRGRFSEQTIGGYQLGTVLGKGAMGEVYEATSPT
                     GAVVAIKLLSQASLSNPNHVLRFLRELRTVAQVRSPNIVEVIEVGENPLPYLVMEKLE
                     GQTLAEMMRGRRALSPDEVIDLVAQVGAGITAASVAGIVHRDLKPQNVFLDRGTWKVL
                     DFGVARAMDGGDTLTAGQIVGTPSYMAPEQASGGTVDHGTDLYALAAIAYRALTGHPP
                     YAAGEIAETLYRVVHTRPRRPTELVELPPEIDLVLAIGMAKQPAQRFRAATELAAAIA
                     AAFAGTLPESVYERGRQLERGGAWSAGPPARASTNRMRATRPPR"
     gene            2529..3389
                     /locus_tag="VMZ53_31210"
     CDS             2529..3389
                     /locus_tag="VMZ53_31210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33028.1"
                     /translation="MRLLRPLLFAALLASCHPSATVQSTMPIANMQTYSTVALNVRST
                     AFASQGQAMMLEQQVTQKLQQKCSFVSIQRQGSGPPADVVLDLNVTNTGRGSGGIISN
                     SSTAFIDTLLVLSEGQSGELLGSVRIHGTSSGMIVNNADPANEAAEVIAKTIAEMFAK
                     SGCSGARVAKQEPPPVDNGQGSGAGSGSDGTGSPPDDAKRGQAEALNQEGTDKLRSAD
                     VSGALTAFQQANALVADARYVYNICLAYEAGQQWPNAEQSCKQARTMTTDARLLGKID
                     KRLDLIAHKQ"
     gene            3812..4162
                     /locus_tag="VMZ53_31215"
     CDS             3812..4162
                     /locus_tag="VMZ53_31215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33029.1"
                     /translation="MSSRSWREDNRVRQNELWRNWYAKNAKKKVAWGKRHRDEVRAWF
                     RELKATLMCEICGESSPECLQFHHLHAASKRFNLSIASSNGRSPKAILAEVAKCRVLC
                     GNCHAKLHWEERNK"
     gene            complement(4163..4235)
                     /locus_tag="VMZ53_31220"
     tRNA            complement(4163..4235)
                     /locus_tag="VMZ53_31220"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4200..4202),aa:Pro,seq:tgg)
BASE COUNT          769 a         1427 c         1369 g          694 t
ORIGIN      
        1 gagccatgtc gactacacga actgtccggg gcagcgaccc gcaggcgcgc cgctcgaagg
       61 cagctggatc gacgtgccga acagccacgt acgcatcttg ccggtctgct cgatgcatcc
      121 cgcgcaggtg ggcccgtatc actttggcga aggcgcggtg gcgcaggatc agtgcgcgct
      181 gccgaccgcg gcgtcggggt ggctcgaagg ccagacgcta gcgctcgtga tcgattttct
      241 cgacgaggat cgcaagcccg cgttccgcgt gttctatcaa gacgcgccga ccgatgcgcc
      301 gatcggccac gtgcccgcgg tgatgctcgc cgataaggcc gtcgacgttt cactgctgtg
      361 cgtcggcagc tcggactccg tcgaggatca tccgacgaag atcgtcgcga acacgaagcc
      421 gcgctttgcg ctgtcggggc attgggaaga ctttctccag cccgtcggcg cgccgccgaa
      481 accgattccg ctgctcgatt tgcagggcta cgtgacgcgc gccgaagccg cgctgcccgg
      541 cccggccgac gcaccgctcg tcgtcgacgg ggagccgcgc acgacgcggc acgtgctcgt
      601 cgacccaggg gcgcgtttcg tggttccgcc cgcgccttga gcgtatcgta ttagagtgag
      661 ccggcgatcc gacgacgaag gggacaccca ggtctcgtcc cccaagctcg cgatcgccga
      721 cgagaacatc gcgacccaga cgtcgtcatc gaagctgccg atcgatcgca tcgcgcatgc
      781 ggcagcggcc gtgcagcggc cgagtgttca cgacctcgag acgtcgatcg cgccggacgt
      841 ccgcacgaac cctcgctcgc gcccgccgac gaagacaccg tcgacagccg gaggcagcac
      901 gaccgtcggc tccccgctcg aagcactcga gcgcgacgag atcctccgca cgcgttggtt
      961 ctgtttgatc gggctcgcgg tcgggctggc cggcggcgcg tctgtcgcat ttctgcccgg
     1021 cgatccgacg gtcagcttga ttctgctgac ggccgtcgcg gtcgcgatct gcgccgtcgt
     1081 gttcttgtgg atgcgcacgc gcgatccgat cgcgtttcgc aagccatcga cgggccttgg
     1141 ctggttcatt cccgcggcgt gcgtgacctg cgcgattccg ttctacggcg cgttctcgcc
     1201 cgtcgcagtc gtcctcgtat tgggcgtgta cttcaccggg cttggcaaga gtacgaagct
     1261 cgcgatggcg gtctacgcgg tttgcgccgg catgcaggcg ttcatcggaa tcctcgtgat
     1321 cgccggcatg cgcgacaccg gtatcgtcca cgtcgagttt ctcgatacgg agacgaagct
     1381 cgtcattcaa gcactcgtgc aagtcgtgct gctcgcgacg ctcatcaccg cgcgcatgtc
     1441 gcggcgcacg gcgctcgcag ccgtcggtga gctcgagcgc gtcgtgcggc aggtcgctca
     1501 tcgcgaggcg ctgctgctcg aagcacgtga ggagctcgag cgcgcgctgc ggcaaggtcg
     1561 cggcaggttc agcgagcaaa cgatcggcgg ctatcaactc gggacggtcc tcggcaaggg
     1621 cgcgatgggc gaggtgtacg aggccacgtc gccgacgggc gcggtcgtcg cgatcaagtt
     1681 gctttcgcaa gcgtcgctct ccaatccaaa ccacgtgctg agattcttgc gcgagctgcg
     1741 gacggtcgcg caggtccgct cgccgaacat cgtcgaggtg atcgaagtcg gcgagaatcc
     1801 actgccgtat ctcgtgatgg agaagctcga gggccagacg ctcgcggaga tgatgcgagg
     1861 gcggcgcgcg ctatcgcccg acgaggtgat cgatctcgtg gcgcaagtcg gtgcaggaat
     1921 caccgccgca tctgtcgccg gcatcgtcca tcgcgacctc aagccacaga acgtgttcct
     1981 cgatcgtggc acatggaagg tgctcgactt tggcgtcgcg cgcgcgatgg acggcggcga
     2041 cacgctgacc gccgggcaga tcgtcggcac gccgagctac atggcgccgg aacaagcgag
     2101 tggtggcacg gtcgatcacg gcacggacct ctacgcgctc gcggcgatcg cctatcgcgc
     2161 actgacgggc catccgccgt acgccgccgg cgagatcgcc gagacgctct accgcgtcgt
     2221 gcatacgcgc cctcgccgtc cgacggagct cgtcgagctt ccgcccgaaa tcgatcttgt
     2281 cctcgcgatc ggcatggcga agcagcccgc acagcggttc cgcgccgcga ccgagctcgc
     2341 cgctgccatc gccgctgcgt tcgcgggcac gctccctgag tcggtctatg aacgcggccg
     2401 ccagctcgaa cgtggcggag cctggtccgc cggccctccg gcccgtgcat cgacgaaccg
     2461 catgcgcgcg acacgaccgc ctcgctgatt cagacccacg cgtggcgcga tgcctgtatg
     2521 ctccgcgcgt gcgtctcctt cgccctctcc tattcgccgc cctgctcgca agctgtcatc
     2581 cctcggccac cgtgcagagc acgatgccga tcgcgaacat gcagacgtac agcaccgtcg
     2641 cgctgaacgt ccgctcgacg gcctttgcct cgcaaggcca agcgatgatg ctcgagcagc
     2701 aggtcaccca aaagctgcag cagaaatgca gcttcgtgtc gattcagcgc cagggctccg
     2761 gcccgccggc cgacgtcgtg ctcgatctca acgtcacgaa caccggccgt ggcagcggcg
     2821 gaatcatctc gaactccagc acggcgttca tcgatacgct gctcgtactc tccgagggac
     2881 agagcgggga gctcctcggc agtgtacgga ttcacggcac ttcgtcggga atgatcgtca
     2941 acaacgcgga ccccgccaac gaagcggcgg aagtgatcgc caagacgatc gctgagatgt
     3001 ttgcgaaatc cggctgcagc ggggcgcgcg tcgcgaagca agagccgcct cccgtcgaca
     3061 acggccaagg ctcgggcgct ggcagcggct ccgacgggac aggctcgccg cccgacgacg
     3121 ccaagcgcgg ccaagccgaa gcgctcaacc aagaaggcac tgacaagctc cgcagcgccg
     3181 acgtcagcgg tgcactcacc gccttccagc aagcgaacgc gctcgtcgcc gacgcgcgct
     3241 acgtctacaa catctgcctc gcgtacgaag ccggtcagca gtggccgaac gccgagcagt
     3301 cgtgcaagca agcacggacg atgacgaccg acgcgcgcct gctcggcaag atcgacaagc
     3361 gcctcgacct gatcgcgcac aagcagtagc tgtccgaata ccggacacgc ccccgcgcgg
     3421 gctttgtcag accgccgacg tagggtttcg cgcatgcccg cgaaggatcg cgaccagcgg
     3481 cgcgcaacct ggaacgcgtg gtacgcgcgc acaaagaagc aacgtcgaac gccggaacgc
     3541 gtcgagcacg aacgcgtaac caagcgcgca cgccgacacg cgatcgttca atggctcgtc
     3601 gagctcaagc aacagctgac gtgtgctcgc tgcggcgaag accatcccgg ttgcatcgtg
     3661 ttccatcacg ccgatccgcg aaacaaggag gtagctgtcg ccgaggccgt ccgtcgtgcc
     3721 tgggggcgca cgcggctctt ggctgaaatc gcgaagtgcg aggtcctatg cgtcaactgc
     3781 catttgaagc accacgcgag gcaggtactc gatgagtagt aggagctggc gagaagacaa
     3841 tcgcgtacgc caaaacgagc tttggcgtaa ctggtacgcc aagaatgcga agaagaaggt
     3901 cgcgtgggga aagcgacatc gtgacgaggt tcgcgcctgg tttcgcgagc tcaaggccac
     3961 gctgatgtgc gagatctgcg gcgagtcttc acccgaatgc cttcagtttc accatctgca
     4021 tgcggcaagt aagaggttca acctctccat cgcaagctcg aatggcaggt cgccgaaagc
     4081 cattctggcc gaagtcgcga agtgccgcgt gctctgcggc aactgtcacg cgaagcttca
     4141 ctgggaagaa cgaaacaagt agtcggggtg agaggactcg aacctccggc ctgaagttcc
     4201 caaaactccc gcgctaccaa ctgcgctaca ccccgtagct gttcggatac ctggtatct
//
LOCUS       DATLBZ010000636        46102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7926498, whole genome shotgun sequence.
ACCESSION   DATLBZ010000636 DATLBZ010000000
VERSION     DATLBZ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 46102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46102
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7926498"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="VMZ53_31225"
     CDS             <1..1369
                     /locus_tag="VMZ53_31225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33030.1"
                     /translation="AGRFPLGQPAHEAFEKWARGMAEGGIYDHLGGGFARYSVDAKWL
                     VPHFEKMLYDQAQLLGIYATVCAIGGPFATRAGEVLAETVAFLERELSDPQGGLWSSL
                     DADSEGEEGKFYVWTPDEVRAALGPVNALVFCQAYGVTDAGNFEHGATVLSRVTPRSS
                     PHEEEQLAELRARLFEARGKRVRPGTDDKVLSGWNGLALTGLCAAWRATGHAPALELA
                     KRVGGFLRDHMIRDGRVARVFHEGKTKELDGTLDDYAFCAQGLLDLAEATGDGAWWKL
                     AEGLLATVRERFVEEDAGVVVFYMSPAGDSLLVHRPESHHDGAIPSGASIAVQALLRV
                     GLVAGDAAALALAEKYLAQRLASSTQVNAWSTSSLLAALDLYLHVQELVVTDGTGRDA
                     LLASAREAYAPTLAVIGPWAQASILDGKTADGDRSRAFVCKGPTCSPPVTEPAELVKL
                     LNSTG"
     gene            complement(1353..2939)
                     /locus_tag="VMZ53_31230"
     CDS             complement(1353..2939)
                     /locus_tag="VMZ53_31230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33031.1"
                     /translation="MRFLVAALLLVAGCGGCGDDALTPDGGDDAIDVDADPNVRGMVT
                     VHVVDKLDGPVSGIDVVFIDTDATVTQAVTDASGTVQASVFPNASVTAIRERTGGNSY
                     SMTTLLALNPGDDIELVTAWEAASSGEDPFSVRVLPLPSADLVSAAKSGSTATFTTVQ
                     PHGLAAGDTVVIQGTAPAGYNGTWTVATATPTSFTANLGGGGLGNATTLGTAAKGYPF
                     TVNYTAATGAQGYTVYTACGATDVGTTLAPQVVMLAGCTPATSTVVVVARGGGGAPIS
                     YAQKTDVAVTAGGNTTITDSWHAMDTLAVTYSNPTTRVTDIVAERYVPYIRTPPIAMG
                     TGMTMNMATSSSATAFMKTRVRCPTGAAPDCLSNNIGSVSQTITERVDGTLASYSLDV
                     GANLLPWVTATYTPGTTTIDVSVTGTGAIDLFEANLRYAGATGSPRGSTIYTWRVFGP
                     IAQSVTFPTLPSTIAGDPTVRATDVQSQFQVYLCETEAVNGYRAAVKNPYETLATCEA
                     NGTPTTKPLTAAKSRLSQWN"
     gene            complement(2950..3357)
                     /locus_tag="VMZ53_31235"
     CDS             complement(2950..3357)
                     /locus_tag="VMZ53_31235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33032.1"
                     /translation="MAEPQEREFMGGQKSKAWTYLILMVLLVVIAIVVNLVWKNPDAA
                     RNGVHTFLGLPAWALATVAFVVGALIFWLGLKIETDWPEAIGAFVISASVAAFEIIVG
                     WDRFALGGIVVLPYLLPLVVFVLLLMYAMRRSA"
     gene            complement(3369..6437)
                     /locus_tag="VMZ53_31240"
     CDS             complement(3369..6437)
                     /locus_tag="VMZ53_31240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33033.1"
                     /translation="MASKDGDPPPNAASSAAFAATIAPADVPTPPEKLAGGTLIEDSG
                     LEATVAAPASREPGDKTSSEPELVEVSTVHYQIDRELARGGMGKIVAAEDRRLHRRVA
                     LKQLLDPAGEHITRFQREALITARLQHPGIVPVYEAGRWPTGEPFFAMKLVSGRPLDK
                     VIGEATRLEDRLAIIPRLAAACDAIAYAHSQRIIHRDLKPGNVLVGDFGETVVIDWGL
                     AKDLDAADTHESANRSPKQAVRKKDEVTTVPLPGSSTLTVAGAVMGTPAYMAPEQARG
                     EPLDQRADVFSLGAMLYHTLCGVPPYNARTATDVIAAAALGKIVPLREREKQAPADLV
                     AIVDRAMAPSPYDRYPHAGELADELRRFLTGQLVGAHRYTASQRLARFVRKHRAAVTI
                     SAIAITAMTVGGTIAVRNIVDARDRARREEQIAVTRKQAAEHLIDYMFDEMKTRLSAA
                     GRLDLLSNMGQEVKSYYAVLERAPGGMSREDEIRMAQAIDLIGASSRVSGKPDEALET
                     WKQAREHLIAVVGNDKSEHSRRLRFMIAQFDFQEGRVFQERGKSAEAQTHLQRAEAMF
                     DELARGSLNDKEILLAAAETHDHLGDLLRIDGKIDEAFEQYNEAKNEREAATSTGNGR
                     PSVELLALSTSHFKLGAVFQNRGESGSALEEYRLALRQRQTLLEAAPDDVERQAAVLE
                     VDRELADLQRATGDENAAIETYKEAIPLAEALVHRDPTNTEWQYQRGNLMSDLGYTLS
                     NTGAYKDALVQLGYSIEVQKDLVARDTKSSRYKIALSRSHTRVGDAHLGLGKFPDALA
                     AYRLALEIRSDLVDADTKNVAYRRSVAFSYAKLANAFTAQGNTAQALEAHEQAFALRK
                     QLVAESPAQGGFKNELAWSEVRLARLLASSDQKRATALIKSGLDRARMLVAGDSINQE
                     FKDTLTEGLLASATLSKAIGDETERRALLMEALGIAADAANTAKQNANWPGHLAEIHA
                     GLAEHAAATGDAKTAKAEWKAVRDVLEPLAAADHLAATRRPLLERARAFR"
     gene            6485..7180
                     /locus_tag="VMZ53_31245"
     CDS             6485..7180
                     /locus_tag="VMZ53_31245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS33034.1"
                     /translation="MIHGSAADRTTWSIQLAGPLREKFELTAYDRGELPSVEAYAAEA
                     ATRIGDSRALVVGSSFGAVVALELLRTQPQLCAGAILIEPPMAPTDAPPTDPVAFLRD
                     FDLIVSEQGGPAAGEFFLRTVLGDAAFERIPKAFRDRSAAKWAAIRADSQALIEYRPR
                     YAELATVTTPVLLLGGDRSAPYFRPTLDALARVLPSAKLEIVSGAGHMLHAEAPRRFA
                     ELLIAFAAEIQIG"
     gene            7194..8618
                     /gene="hflX"
                     /locus_tag="VMZ53_31250"
     CDS             7194..8618
                     /gene="hflX"
                     /locus_tag="VMZ53_31250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HUS33035.1"
                     /translation="MTIDGGTLFDRLGERPPTVVVAVQLPGVTEGELASSIEELERLA
                     KTLGLDPVARITQRRAKLAPGVVLGEGKLKELAEWTGGTGEIASFESTVARRKRREDE
                     ALEAEDEDDDDDETEDDDSEPGTKPTAKAKVVLVDHDLTPSQQTNLEKATGVDVLDRT
                     SVILEIFHRHAKTREARLQVEIARLKYLAPRLRESGGGGDRVRGGIGGKGAGETSLEL
                     DRRRIRDRIAELRDELSRIEGGAKTRRDRRSELPTVALVGYTNAGKSSMMRALTSTDV
                     YIADKLFATLDTTVRRLVPPTEPPILVTDTVGFIKKLPHDLVASFRSTLDEAKEADLL
                     LHLVDASDPAHADQLAVTREVLAEIGADEVPTWLLLNKIDRVDEAERAKLADKYPGAI
                     QLSAKRTSDVGVLRDRLIEEFIGALEEAEIDVPWSHQRLIHVMHETTTVLSETHHDDG
                     TRVRLRAPAGTIEELRNQLKEDAR"
     gene            complement(8599..9108)
                     /locus_tag="VMZ53_31255"
     CDS             complement(8599..9108)
                     /locus_tag="VMZ53_31255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33036.1"
                     /translation="MRFVCLVALLAGCGVANFDIEEPVPEQRVTGSPLPGPLAVLFPL
                     PLSIDLSSKIKAMETGPIDSVTLSKLTLSITATAMTGTDMDDWSFVDEIHVFVKSNKS
                     GSTLPRIEIANISHPGAVKTLEFHCDSSVDINPYVNEGSVVEGESSGRLPEDDVTYNG
                     EGVFTVHPL"
     gene            complement(9155..10462)
                     /locus_tag="VMZ53_31260"
     CDS             complement(9155..10462)
                     /locus_tag="VMZ53_31260"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HUS33037.1"
                     /translation="MSRAILLALLAVGCGRQLNPEYCAANPNDVECRAADLVMVDAPV
                     GCPMIACTDQKQPVCEVSSGNCVQCLPPGMMGSDPMQCAADETCGQDRKCHACVVDTD
                     CKDSKVCLSDGSCAMQSNVLYAAPAPNGTGNLCTIDMPCTFTTAVSLLGAKSIVKLTT
                     TKGTDYSEPPIVVAASGTIIGTGATFIPMGAGPAISMTKQTNVEIVGLTISGATAQGI
                     LCSETKFQLRRATIIGSGGYGVQTTKCDATIERTKLMRNQAGGMFIADGTFEIRNNFV
                     GPDNGSSALTQGNIRTDKAKGRIVFNTVAKNNSENGGGDRFGALKCGGSPDLLVAHNI
                     FTSNGAANAEVGGDCDYFSNYVGTSNEFAEIKFASATDFHLTMMSPTSNPVIRDDPDA
                     DRSTSPAYDCRVTATDSAAYLDDIDGQARPYLLCDRGADEWRP"
     gene            complement(10500..11261)
                     /locus_tag="VMZ53_31265"
     CDS             complement(10500..11261)
                     /locus_tag="VMZ53_31265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33038.1"
                     /translation="MTRVVVILAVLLAAPAVRAETPDVGVLVVGDTPLHGKLREHARA
                     WVKSKGYMLVKAPMSEDAANSFDNCFVLEDMKCARGVFDNRSRSATLIYIGFDPGDPD
                     AKTKPSLKVYWFTKEQPTSGRSVPCPDCDATFEKIVDDELVTLQAMHADLPVTKRPKA
                     SKVWPMLLLGSGIAAIGTGGVFLFYGTYGTALHDRDHKYTYPDAILPGIAFTAVGVGA
                     AIGGIILLKQANARQSAPIASVGPGQAYVGWVTRF"
     gene            complement(11288..12448)
                     /locus_tag="VMZ53_31270"
     CDS             complement(11288..12448)
                     /locus_tag="VMZ53_31270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HUS33039.1"
                     /translation="MKRILFATLALAACSKDNPYYCENAPDHNCMATDTPDAPVSTGC
                     TASTECTSESSPVCGDDHACRACELHSECVSAACLPSGACGSDVTVAYASTAGNDANP
                     CTNEQPCRTIGAAVATGKPYVKLAGSFDEAVSLSGANVHILADAGTTLTRTASSGPVL
                     TLQGTSNVAIEGLIIRDGLGNTGDGIRVPSGEAVKLALDHVSVIDNSGVGVNVLGGTL
                     KMTSCTVSGNKTGGAIIMAAIDITNSLFVANGASTSTTGGLTMTPSGSQNAFRFNTVA
                     DNVSSSSTLALRGINCAIPMTISSTIVSGNLASTYCMFDYSLLASPTGTNISGDPKFK
                     ATDATNPLAADYFRIQASSDAVDHAEPGAAMSTDIDGEPRVGAKDIGADEAQ"
     gene            complement(12448..13251)
                     /locus_tag="VMZ53_31275"
     CDS             complement(12448..13251)
                     /locus_tag="VMZ53_31275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33040.1"
                     /translation="MRTAFVVLALAIATPAHAEQVGVVVTGEATLQPQVASEVEVWLH
                     ARGRTIAPAALDPSAVNTLIDCFVLEDIGCARGLIDARSKATSVIYARIDATQGDDGT
                     RQVEVVGTWFQKNHESMSERRLCDKCTDEKLHTTIDELMLALVHQPPLPAESAPPPAP
                     PVEETTPVMPVSEGQPRRNMLPIGLAAAGAAALLTGGVLVAIDEDPSPTGPQQPSYRD
                     SGTAGFVIGFAGAAMIGTGVYLWLQDRKHGAPVASVTHDGAVVGWTGRF"
     gene            complement(13254..14882)
                     /locus_tag="VMZ53_31280"
     CDS             complement(13254..14882)
                     /locus_tag="VMZ53_31280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33041.1"
                     /translation="MLGQTGKYRLDQRLGGGGMAEVFLGSMLGAEGFQRRVAIKRVLP
                     GFSENPAFSQMFIGEAQISSQLQHANIVSVLDFDRDPEGRLFLVMELVEGKDLDALAS
                     SGPLPLPVIIYVIAEVLRGLGHAHDLPVGAGMRGIVHRDVSPHNVLLSWDGAVKVSDF
                     GIAKAKQASEATASVFIKGKPAYMSPEQANGQSLDGRSDLFAVGVMLWEMLCGRRLFV
                     GEDTRATLAAVLFGQIPRPRSMRSDIPKDLERVVMKLLERDLPARYATAEDAIHDLFE
                     CSDAPKAGRELLMRTMAERFPGQAPVRQSVARRGGTPPQGQAPQQGGPALGYASTQTP
                     QGYIQANHDQQTIAGYVGQATPMPSIGAARSTPTGTIQPSAVIPRRGGGKKVAIIVVG
                     AVVAVFVVVGIVASTQSGKEPVVAAGSGDGSAAGSVAVPADVAVATVQPDAAAPDAAV
                     EVAAAPVDAGQKDATSTQTGQAGKEKRTGRLVVHAFPVQTVWVDDRKVHDTPVDMKLS
                     VGKHKVKLVNSETGKTEQLSVSIDESKPTTIDRN"
     gene            complement(14933..15127)
                     /locus_tag="VMZ53_31285"
     CDS             complement(14933..15127)
                     /locus_tag="VMZ53_31285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33042.1"
                     /translation="MSLSLLPLLVVEEDVPASVRDAIRKANEAPAAQRHAFLLEAANG
                     LYREVHLDCDDARELVGLDT"
     gene            complement(15124..16560)
                     /locus_tag="VMZ53_31290"
     CDS             complement(15124..16560)
                     /locus_tag="VMZ53_31290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HUS33043.1"
                     /translation="MMDATITSDHKIPSRIIGLVAVLLGQFMLVLDATVVAVAMPTIQ
                     QDLALSPGSLTWINSAYLIAFGGLLLLFGRLSDSIGRKRIFLLGIGAFTVASIACGLA
                     PSATTLIIARFIQGIGAAAASSVILAIIATEFPSPADRAKAMSGYMFVSVSGGSLGLF
                     VGGLLTQVLSWHWIFWINVPVGAIAALLVHRNLREAARPKSRAPIDVAGAVLVTSAAM
                     VAIYGLVEAGHTSWTAPAVRVSLALALVLGVVFFVVEAMRPNPLLPIRMLRIRSLVVT
                     SVVRGFMVMGLYGVFFLATLDMSNTLHFGPLKVGLAFLPQTLTVASLSLGGSAWFVRR
                     FGAVRVLVAGLGIAAIGLGIMAALAIHEPYFPLRAIAHVLLGFGFGLSFLPLLTLAMA
                     DVPAPDAGLGSAIVNLSMQMAAAVNVALLVTAASYRSRVLVDAGISLHDATVLGYRFA
                     YSVALVGVLVGLTLAATLLRKPTERSPL"
     gene            16625..17035
                     /locus_tag="VMZ53_31295"
     CDS             16625..17035
                     /locus_tag="VMZ53_31295"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUS33044.1"
                     /translation="MASSRNVPHILNLWRRVASRATEESMGMNLKQVTMLANLRDKGE
                     LPQTTLCDMMKLTQNTVVTWLNELEDAGYVARIRDPEDRRKHNVALTDKGAGALERAE
                     TELRRMEDEALSGLTADERTQLRKLMAKALEAVD"
     gene            complement(17053..17604)
                     /locus_tag="VMZ53_31300"
     CDS             complement(17053..17604)
                     /locus_tag="VMZ53_31300"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HUS33045.1"
                     /translation="MSLARDTFCSFCGTKYDVLTYPRTCPGCKTQVWANPIPVCVALV
                     PIVTSEGTGLLTIRRAIPPAIGMLALVGGFLEEHESWQTGCAREVREETGIEIDASKL
                     VPFWYASSEPKPNRVMLFAVAQELRADALPPFAPDSETSARGVILGPAGLEEVFAFTT
                     HIEAARRFFAERGVARMHGFFEL"
     gene            complement(17601..19148)
                     /locus_tag="VMZ53_31305"
     CDS             complement(17601..19148)
                     /locus_tag="VMZ53_31305"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HUS33046.1"
                     /translation="MATTDQSEAARILVVDDERVIREILAEFLTLEGFSVNTVEDGEK
                     ALTELRLHPYDLLITDLKMPRLSGLQLLEKVEAERLGVLTVLMTGFGTVETAIEAMKK
                     GAYDYLLKPFKVEEVIHVVERALYRQRLQAENIRLREALTIYKVSEAIALSHDIERIL
                     DVVLRAAIDEVKADVATLHLRDPRTGHYEERVKVEAADAQPHASLPSPAFGILTDQFS
                     AGTPILAHGGKASRFFTESAAPADLASFVAVPLTVRGDMVGVLNVFSFTSGKKFDEGH
                     RKMLAVLASRAASAIDNARLYGEIKEKNTSLERANLSLEEMFQQTVAGFAQALEESDM
                     YTRGHSERVAVYSEVLARGLTLPDSEIRRIVQAGVMHDVGKIGVRYDMLNKPGKLTPE
                     EIAVFRQHPEKGKRILEPVPCLHGLIDGCWCHHEWFDGGGYPRGLSGQNIPLVGRIVS
                     IADAYDAMTSDRAYRRALAHEVAIGEIERCAGTQFDPELSDAFVKLIEAWRLQMRERG
                     HDSLIPR"
     gene            complement(19155..20024)
                     /locus_tag="VMZ53_31310"
     CDS             complement(19155..20024)
                     /locus_tag="VMZ53_31310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33047.1"
                     /translation="MRGLAKLVFVGLFVAACGGGGDGPPGPLAKHFDDMYIARIPLDQ
                     KGGVVETQNQYSIAKMENANAEANEQEADGQLRQAKNDYSAAKLAVDSAITAKKSAEQ
                     SADMNRMNNAQKDLKAAEDSAEAAKLRVKYFEAYRDYLKRYHRYTLENMYWREAQFES
                     AKATLAKQNNIAPKGVNYDDFPKQLEARGKRTTSSKDKAEQFKTKAVSARSSWLSAQQ
                     AADKQTGRTGNLYDPMAQKEGSPTLSGGGMSQEKKDPGQQQPINSNPTPQPQQGGGGG
                     TDGGGGGGTAPQQ"
     gene            20078..20857
                     /locus_tag="VMZ53_31315"
     CDS             20078..20857
                     /locus_tag="VMZ53_31315"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HUS33048.1"
                     /translation="MTTLLAVDVGNTNTVLGVFRDNELAAHWRIQTVAQRTSDEYAVL
                     LKTLLDLDGFPWRSIDAGIISSVVPPAVFGLQRFFKQHCGGPALVVGPGIKTGMPILY
                     ENPREVGADRIVNAVAAFEELRSGCIVVDFGTATTWDVVNPKGEYLGGVIAPGIQISS
                     EALYEHAAKLPRVELARPGKVVARNTIASMQAGLVYGYAGMVDALVERIRAEVDWPAR
                     CIATGGLAPLIATETKTIEATDELLTLKGLKILFQRNQAPG"
     gene            complement(20902..21282)
                     /locus_tag="VMZ53_31320"
     CDS             complement(20902..21282)
                     /locus_tag="VMZ53_31320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33049.1"
                     /translation="MPQHALLVRGDAIDLSRADFASEHRDGLVRDRGVLNERLERGRL
                     VGGDQGEEIEHARVELLIPERTGENAVGQTRCGLTRALGDLRVSVERELIQDRCLLGR
                     RQRRHAARGHHLRRARTERLLRAR"
     gene            21271..21990
                     /locus_tag="VMZ53_31325"
     CDS             21271..21990
                     /locus_tag="VMZ53_31325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33050.1"
                     /translation="MLRHPQIIGAMYLNKHARMSTVDRAIELAVRNNVRVPGLHAWDE
                     IARSLQGHQSTAESDALFAMAADGIEHDDRVLTEGDAEKPIEDIERLEQLALERTKDI
                     PIQNMTVPAKVRLATLGNAFQRAVLIRDPMKIVQMAAIKSPGVTEFEAASYAGNQTLG
                     EDVIRYIASKREWTKLYGTKYSLCRNPKTPVTDAMRLMPFLREKDLHTLMKSKGVPSA
                     VVAQARKLVMQRRGGNDQNKK"
     gene            22608..23024
                     /locus_tag="VMZ53_31330"
     CDS             22608..23024
                     /locus_tag="VMZ53_31330"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUS33051.1"
                     /translation="MTQGDPAPQLVLPHIGANGDFTTAFDLAQLKGKVVILDFWATWC
                     NPCLKSMPHLDQLQRRHPEIAVVAINIDDPAGARALFDERRYSMQLVFGDQPAQDRYG
                     VSAIPHTVVIDRSGVVRSVFRGGGADLERAIAPLLK"
     gene            23348..24466
                     /locus_tag="VMZ53_31335"
     CDS             23348..24466
                     /locus_tag="VMZ53_31335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33052.1"
                     /translation="MRLGTCIALELAVAAGAIGGLWIGVGHTVRLADEYVAERDASAA
                     TLSDRHTRLAPTRVPGGWLAVQPKSAPATVFDAPDDALLAPLAAAPVTRVKINRGGTS
                     LSLRIDFANGSRCAFKPEQIHLHSNPRKEIAAYRIDRLLQIGHVQPAKAIRLRLADLV
                     AAADPDVRAVTAQRLHDEAIARPVDPADPATLELRGVASWWITDIRDAHLGPALVDQA
                     EGMDLWTQELKVGAPIPAQHRVLVEQLSTLVVFDVLIDNFDRWSGSNTKASPDGKILY
                     FMDNAIAFSKYTQGHDMNLGPFRRIQKFSRGLVERLRALNTESVTAALDVTGQDAGLG
                     PLLDADQIAAILARRDYIVGYVDRLIAQYGEDAVLAFP"
     gene            24463..25803
                     /locus_tag="VMZ53_31340"
     CDS             24463..25803
                     /locus_tag="VMZ53_31340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33053.1"
                     /translation="MTPCPVCGKPVDPLRAPAVSVRDGKVVAFCSKECRAQAETQPVR
                     IQPTKVVRLPSEDAPIVGRTATADKLRTPSTGVPSSGVPKSTADLDSGPVIEIVREPS
                     RPVVVEPTKKRKRSDSSIQIADTGRLDDYIVTDGGGRGRILLAVFLLGLLVGGGIAAY
                     KLGYLDGLLNKKPARAAPGDAAVAPVFDDAPPAPAITPDAALAKAKSVLEAQLKSDSP
                     RVQRVAASALARTGDKAAFDLLAKSIATESSDVAKLDMAYALARSGDKFGTDALVVML
                     GSGKRDPKLEAGRRLALLGDKRAVNALGSYLSVDQLRLGTAEQLAYLADPKALPDDKA
                     RATIALGHAGRTDVAPALHELLTDDRQNAFAAQALANLHDEAARPVLVSQLGIPSLRV
                     KSARALRRLAPDADVSALLPPLVAALDSNKDTEQVQIAEAVLLLAGPAAWSEHE"
     gene            25800..26990
                     /locus_tag="VMZ53_31345"
     CDS             25800..26990
                     /locus_tag="VMZ53_31345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS33054.1"
                     /translation="MSRTLRLFYLFRLLATSYLYVPIFMLFQAQRGLTFFEQLALGGI
                     YSAVVIAVEIPTGVFADRIGRRLSMMAGALMMSASCLVAASAHGFGTFVVAECLAAIS
                     MALCSGADSAYLFDLLLEHDRVHEYPRRESAASAWHLMGSACAFAGGGLLARVDLALP
                     YLVTAGVAAVAAAVAFMLDEDRPRDRARAPAMRVMRSWSREMTAALGDVARNGRLAWL
                     IGYSAVVFMLLRATLYVYQPYLHARGLGPIAIGVLFAGVYVVASAVAYRTHLLRRRFG
                     DETLIWALLAALSISFIGLSGVAQGPWMLSLLLVQAVANGMYSPLTKPLLNAEITDSS
                     RRAAVLSVESMARRFAMGLFAPLVGLYGETDVMMLCGVVGLVGFALLGVARMRRSVAD
                     TYSA"
     gene            complement(26978..27748)
                     /locus_tag="VMZ53_31350"
     CDS             complement(26978..27748)
                     /locus_tag="VMZ53_31350"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HUS33055.1"
                     /translation="MTPHRLDGLTALITGAGQGIGRAIAARFVDEGAKVVLFDNNKRA
                     VEDAADELGERTRAVTGSIADEAAAQHAVDAAVAFGGGLDIVVNNAAIADPDNGPPEA
                     LGLDKWREVIETNLTGTFVVSRAAIPQLRERRGAIVNLTSTRAFMAEPNNEAYAATKG
                     AIISLTHALAMSLGPDVRVNAIAPGWIATDAWKPRNERKPPELRKVDHEQHPVGRVGR
                     PEDVAALAAYLASSEAAFVTGQTFTIDGGMTVKMIYAE"
     gene            27832..29205
                     /gene="rimO"
                     /locus_tag="VMZ53_31355"
     CDS             27832..29205
                     /gene="rimO"
                     /locus_tag="VMZ53_31355"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HUS33056.1"
                     /translation="MAASAQRIYFVSLGCPKNQVDTELMLGQVEAAGHALVHAPDEAD
                     VIVVNTCAFIDAAKEESVDTILEMAELKKGAARKLVVTGCLAQRYADDLAKDIPEIDH
                     ILGSSDFQSISKVLGAQPSKATKGRKKLPMVQVTETPEYIYDHEAPRVRIGANHSAYV
                     KIAEGCDRPCSFCIIPKLRGPQRSRRIDDIVAEVAQLGREGVQEINLIAQDLTRYGWD
                     AGSSPESRQTLAQLLRALGGVDGIQWLRLHYTFPSAFDNDLIDAIASEPKVVKYVDVP
                     LQHISDPMLKRMRRGHSSRVTRELVKTLRSRIDNVVLRTTFIVGHPGETDAEFDELCE
                     FVAESKFDRAGAFTYSIEPGTTSAMLPNRVDPAVAAERQQKLMEIQRQISRARHEAMV
                     GKTIDVLVEGESDESEHLMEGRWYGQAPGIDGVTYLADGRVAPGSIVKARVTQASDYD
                     LAASLEV"
     gene            29267..30178
                     /locus_tag="VMZ53_31360"
     CDS             29267..30178
                     /locus_tag="VMZ53_31360"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUS33057.1"
                     /translation="MKWLLALVALCGVAHAGDDSCVDDGKPYDQKALKARIEYLASAD
                     LGGRVPGTEGDKLARAHIVARMKCLGLTVSEQAFKDNEKKDTANVIGILKGSDDSGEI
                     IIVGAHHDHEGNGHLGANDNASGVTGLLAIAQAMAQKDAKPKRTIAFIAFGAEEEGDL
                     GSQFYAAHVPDELPMDKVVQLINLDMIGSYKSKSVVAAMGTFAKFPARKAIDKLVKGY
                     PKTRFALGGHGYRSDHEAFCQQGIPYVFFWTPDARCYHQKCDKPSAIDLPHMADIASL
                     AGDLTWELANSETDLLASRTKLGCGQK"
     gene            complement(30179..31180)
                     /locus_tag="VMZ53_31365"
     CDS             complement(30179..31180)
                     /locus_tag="VMZ53_31365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33058.1"
                     /translation="MRVAGMIALVGSVALVPAIASAGGRHHDDGGDVRDHRTSSSSSD
                     SNDAPVVRDHRTHDSDSHDAPIVRDHRTHDDGPVVRDHRTHGGSSGDAPDVRDHRTHD
                     SGGDVYYSSGSDVYVEDSSPGSNPFANLTAPTWSLELGGFAHRFRGPALKHSGQLETY
                     DGDMASYAIGSGDASKGDTAGGTFNMRFTMPVSEHLYAGAELGIGGLTRTPVQLMTNS
                     PELHMTTKALIDTDAVIGARTRSGRLELDGEVAAGVRVVSATVEADGYGASEEEFDQQ
                     ATANGVSGLVEARLRGLLWVSPHVFLAAQAGVGVLERDDVSFGLSIGLTSRPFAKTR"
     gene            31511..31750
                     /locus_tag="VMZ53_31370"
     CDS             31511..31750
                     /locus_tag="VMZ53_31370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33059.1"
                     /translation="MIVGVLLVFAVYLIVAHWTTLLQGGSVVPFVMGFIAFLAAWPAL
                     GWKLALLAFFVDPGSLMCAPWLVASLWEERRAARR"
     gene            complement(31770..33233)
                     /locus_tag="VMZ53_31375"
     CDS             complement(31770..33233)
                     /locus_tag="VMZ53_31375"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HUS33060.1"
                     /translation="MRDRTAICQSALDGNLQGIQYQLSIGAPLDGDGCETYDDQTDGC
                     ITGGAYHFPLGEAARMGHLECTQVLLDGGAPVDGVDYRNETALIMAASADHVKIVELL
                     IARGANVNHVGCLGSALCVVQPGWRPNAIAIAKALLAAGASPLATASYTESAVDKWLD
                     EIARGRVDKKIAPKLLELLRLMLPTAGDGAFRVEEGIATLEKKVGGTNKDAAKAAASY
                     AKLAKVDGKTWPADVKAALGPIEKEAGRAAIEAVCKQVLSTPAAIAHAAWPQLVLDVL
                     ALSKTYADVTKKTYGKALPVSKMGDDEGGAIDYYADEHLYTFDWLLGVLAQPAAIAHP
                     AWPGLFGALCDAKQKAVGYYSFGDDEVAALLAIDQVKWHEQFASVCAAAKQAFPYAAC
                     FGANGEHRDFPTVGAPPLPRVEGASPQAAPAKKAAPAKAPPAKAAPKKAALAKAPPAK
                     AAPAKAPPKKAPPKKAPPKKAPPKKAPPKKAATKKRR"
     gene            complement(33262..33960)
                     /locus_tag="VMZ53_31380"
     CDS             complement(33262..33960)
                     /locus_tag="VMZ53_31380"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUS33061.1"
                     /translation="MSANDRSIPSLMAVTMTLFGVTWKRLLRGRALWVCALIAALPAL
                     VAFGLRGDRDMAELMRFAQFRVMAILPPIFVASSIGEEIEDRTITYMWSRPIARWSLL
                     LGKIATLAPVAAVLSAAGWAIATVITGKPINPQELAAFALGSVAVSLIAAGIATLVPK
                     QGMALAIIYLVVVDPTMGSIPASIREISVTKQVWLMSGLNTDGSATRATITLVILAGI
                     WLAIGLARIRRLES"
     gene            complement(33957..34886)
                     /locus_tag="VMZ53_31385"
     CDS             complement(33957..34886)
                     /locus_tag="VMZ53_31385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS33062.1"
                     /translation="MSVVAANNVSKWYGHVLGVSDVSWTLKGGIVGLLGPNGAGKSTM
                     IKMMAGLLQPSRGTLTVFDKSPFADIEVRRRIGYAPEHEKTWDELTALELVTAMAELS
                     GVPHNRSREAARASLAQMGMSYAETRRVKGFSKGMRQRTKLATATVHDPDFILLDEPL
                     TGVDPKARIDIVEQIKKLGEAGKTIVISSHVLYEIEALTTDIVVIYRGQVLAEGNIYE
                     IRKLIDRHPHRIRVECDKPRQLASLIAPEDHVARIGFERGAVLIETRDPDRCYERIGE
                     AVLASSIAVTTMTSPDNNLGAVFEYLTGEGGRR"
     gene            complement(34883..35782)
                     /locus_tag="VMZ53_31390"
     CDS             complement(34883..35782)
                     /locus_tag="VMZ53_31390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33063.1"
                     /translation="MSVIHDLGYKRYIGTRRSASTRWLVIMRHQIATGWKKWWRYKLA
                     LGMAFITVCIAGGLMYFSTNKIVKSIGGPNDIMVTMADAVIPLSFKFFCTAAFVLSLT
                     LGSTIIASDTQSGAFTFYFVRSIKPRDYMLGKLAGYGFLVATIVLVGPLVLVGLRLGL
                     CETTDELIERASMIPKILALGTLATLLYTAMPLAMSAILPNRRMALGLWAAYYVIIGG
                     IFTVIGMTTHTPIGALDLQYGLQSVTLELFDLKIVAGRGPEFEMMPLVGTLIVIGLQV
                     IVAMGIIWFQVSRDQKTGVGGSS"
     gene            complement(35779..36849)
                     /locus_tag="VMZ53_31395"
     CDS             complement(35779..36849)
                     /locus_tag="VMZ53_31395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS33064.1"
                     /translation="MSTDLPKAQVVSEGSAGTSVRADEDAARSNKPPLIALVGVTKRY
                     GAITALNSVTARIDGRIIGLLGPNGAGKSTLLKSLLGLIPHEGKASVLGLSTQTEGRE
                     IRDRVGYMPEQEAFLSGMSAVELCTYAAELSGLPRTEAMQRAHAALYYAGLEEKRYQP
                     IDGYSTGMKQRVQLAQALVHDPEILFLDEPTNGLDPRSREEMLALITELPERRGCAIV
                     LSTHLLPDVERVCDRAVIMHQGRVRFVGTIDELRGARGRDSELMVEVKADAQKFADAM
                     QAVGASCMVTSPVQVLVNLPPNATSELVFRRAKEAGIQVRGVEGRRESVETAFLRVIG
                     EQAPIVPPPPAPGSITPPGAAR"
     gene            complement(36978..37355)
                     /locus_tag="VMZ53_31400"
     CDS             complement(36978..37355)
                     /locus_tag="VMZ53_31400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HUS33065.1"
                     /translation="MKWVVLLLLVVLAACGGGQKKTTQGKPTGVQEGYATWYGGSLHG
                     GPTASGERFNKNAMTAAHRTLPLQTRVRVTNKRNGRSVVVRINDRGPYGNKSRIIDMS
                     EASARVLGMIEAGVVPVRIEVIR"
     gene            complement(37377..38861)
                     /locus_tag="VMZ53_31405"
     CDS             complement(37377..38861)
                     /locus_tag="VMZ53_31405"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HUS33066.1"
                     /translation="MEEARAKGPVLVVDAGSLLYAKNPLPAHLEAQEDLKANLLASIY
                     KENLKVAAVGLGPADLAKGAGSVRFPREAANVSDPAFKLQAPEVFTVGDAKVGVFGVI
                     ARDTLKDVKVDDPVAAGKRAVDQLRKGGAQVVVALVQASSKRDAVALIRDIGAIDLSI
                     AGLGAVAPEPEDVSVEAEKIGDGWLVIPGNRGQVLSRVDVTLRAGGGTLVDAVGPGAA
                     KVKIAQLDHELGKLDAAIAKFKADPSSDPQFIAKKETERTEVAAQKKQLEAQPLVVPA
                     KGSYFTLEQLRINKQLACSPPVRDAIKGYNAAAGEANVKAAANKVPVPPAKGKAGYVG
                     NEACSDCHQEQVDFWKTTVHAKAWETLVKRGQQFDYECINCHVTGWEQPGGSTLSHNE
                     NLRDVGCEQCHGPGSIHVDKGGMEKPFAVVRAPAKDLCATQCHTKEHSDTFDFTPYMR
                     DILGKGHGEKERATLGDGPTGGQLRKAALDKAGRELGADCVDTQ"
     gene            39142..39390
                     /gene="rpsP"
                     /locus_tag="VMZ53_31410"
     CDS             39142..39390
                     /gene="rpsP"
                     /locus_tag="VMZ53_31410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HUS33067.1"
                     /translation="MAVAIRMSRQGKKKSPFYRIVAADKRAARDGRFIELLGTYHPIT
                     KALKLDMERYLKWLSVGAQPSGTLAAVIRRTARAAKAA"
     gene            39396..39644
                     /locus_tag="VMZ53_31415"
     CDS             39396..39644
                     /locus_tag="VMZ53_31415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HUS33068.1"
                     /translation="MAKEPDLAELIRYLAVNLVDKPDEVRVELVEERQANVYELEVAE
                     SDLGKVIGRGGKTARALRTVVQAVAPRSRKRTLVEILE"
     gene            39669..40184
                     /gene="rimM"
                     /locus_tag="VMZ53_31420"
     CDS             39669..40184
                     /gene="rimM"
                     /locus_tag="VMZ53_31420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HUS33069.1"
                     /translation="MRGDARIEIGGIARAHGIKGEVVVVTHDPDSETLGKITSIWIEG
                     TEYKITGARDTQRGWLVALEGIVTRNDAERLQGKQIEVERSSLELDEEDVLLDDMVGC
                     EVRLPDGTPWGTVVAIDISNPHQDLLVVHDGDTERLLPIIDQFVTNVDLDAGIVMVDP
                     PEGLPSSKVPK"
     gene            40123..42168
                     /gene="trmD"
                     /locus_tag="VMZ53_31425"
     CDS             40123..42168
                     /gene="trmD"
                     /locus_tag="VMZ53_31425"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF021455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HUS33070.1"
                     /translation="MRASSWSIRRKAFRHRRYRSDATRARTMKFKVVTILPELIGPAL
                     RAGVVGRACEAGIIQVYTVNPRDFTRDRHKTVDDTPYGGGPGMVMKAEPLLAAIEYAS
                     EPPDPDSVEATPEADMIAAPVSRVDADLPGDPDDVMSTGRFDRAALRDLADADDGDIM
                     TTGRFTRPSTADLQGRAPAQGDATSRADALARAKAAEEARKKAADDAAKRALAALEGT
                     STDEQGDILDDEAAQRALDELERQARDREAGLHHATTKREEVRGTLSAVDSSIDKLEG
                     ALGGNLDALAPLGAPRASTGSSRSTGSSGSTSSSGSTSSPGSTSSSPSTSASGESAPK
                     PHRILLSPSGKPLTQQRVRELAKLEHLVLVCGRYEGIDQRVIDLAIDEELSIGDYVLS
                     GGELGALVIIDAVARYVPGVLGEATSVDDESFSAGLLEYPQYTKPAELRGQAVPPVLL
                     SGNHAHVAAWRREQAMRRTAARRPDLLRAFVPTKADAKALPPLRARTHLALVHHPVVD
                     RTGAVITTAVTNFDIHDLARSSMTFGLAGYHLITPVTSQREKAQHIAELWIGDEQGEH
                     RARALRLVRAADSIETVIAALTAEHGRPPLVVATSAKPTSFPGAARRTSSELLAEATL
                     DPSPMLILLGTGWGLADHLIPSVSRILAPIEGASDWNHLSVRSAGAVILDRLFGRPA"
     gene            42231..42575
                     /gene="rplS"
                     /locus_tag="VMZ53_31430"
                     /pseudo
     CDS             42231..42575
                     /gene="rplS"
                     /locus_tag="VMZ53_31430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010334229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            42828..43433
                     /locus_tag="VMZ53_31435"
     CDS             42828..43433
                     /locus_tag="VMZ53_31435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33071.1"
                     /translation="MAKTPAKPQQSDPLFAALKALVKPYAKHFKVFEDTDQHYDLEED
                     LRYSRTMFAVLYKRPREVRFYFYPLNVWPDLRELLPASLQSQITGKWIIKVAVLGPAE
                     KKALAKMLAEAWRRIAAIRALAKQHPFYRKFDLEDTHALITKIARKATVELRKKDVQV
                     TLPNTPTVIPAVLGPRVFKPGIFRFKSITPDEHAALAALTR"
     gene            complement(43524..44009)
                     /locus_tag="VMZ53_31440"
     CDS             complement(43524..44009)
                     /locus_tag="VMZ53_31440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33072.1"
                     /translation="MRVGVRLRLAVLACFCFVACDQGEGARATRESERATRETAAAVG
                     AGSEVPPAQHGVGTIKGRVTHTLDGPGRAHAPEEAFSTTTGSECTVSARDAWGKTTDA
                     RSAAETGGFEMRVPAGRYTVSFDACAIPSSCGETERAKPVEVTAGAVTEVSWDCVVHA
                     K"
     gene            44168..45055
                     /locus_tag="VMZ53_31445"
     CDS             44168..45055
                     /locus_tag="VMZ53_31445"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HUS33073.1"
                     /translation="MKSNESTPWSRAVERARGNADDMKPPASNAHLERDNPSMRQLVS
                     KAHADRAPDNPSMRQPVSKAHADRARGNAADMRPPVSNAHLERDKPSVKRPVLPAQRD
                     CEQSSVQPPVSKAQCDRDKPSVKPPVSKAQCDRDKPSVKPPLSKAQRDRDKPSLKPPV
                     SKEQLERDRQVLMFDDAAGPPTTIHRGNASEAHAAAYLERVGMRIIERNFKTRLGELD
                     IIARDGATLVFIEVRSRSTTTFGSAVEAVHHHKQRKVTRIAWQYIKVRRPRFDTARFD
                     VVGITAGELVHIRDAWRIA"
     gene            complement(45240..45731)
                     /locus_tag="VMZ53_31450"
     CDS             complement(45240..45731)
                     /locus_tag="VMZ53_31450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HUS33074.1"
                     /translation="MERALCDSIDIGDIDDDEPGRAKRSISKALKRHVLHRDGYRCRV
                     PGCSATANVDCHHIVFVCNGGPNEAPNLIALCEGHHLALHAGTLIIEGDATNARFTFR
                     AQSKFEMARRAVECAAELRKRGVAKGLVKAAVDATRTHVGSQNLTLEQWVDVALRKVA
                     ALS"
     gene            45886..>46102
                     /locus_tag="VMZ53_31455"
     CDS             45886..>46102
                     /locus_tag="VMZ53_31455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33075.1"
                     /translation="MERVLLPAWLSITQVAAELIPTWVSFGRVDADLIPTWVSISQVA
                     AELIPPWVSLGQMDAKLIPAWVSFAQVD"
BASE COUNT         7689 a        15459 c        14929 g         8025 t
ORIGIN      
        1 cgccggccgg tttccgctcg gccagcccgc ccacgaagcg ttcgagaagt gggcccgggg
       61 gatggccgag ggtggcatct acgatcatct cggtggcggc ttcgcgcgct actcggtcga
      121 tgcgaagtgg ctcgtgccgc acttcgagaa gatgctctac gaccaggccc agctgctcgg
      181 catctacgcg accgtatgcg cgatcggtgg accgttcgcg acccgtgcgg gtgaggtcct
      241 cgccgagacc gtggcgttcc tcgagcgcga gctctccgat ccgcagggcg ggctgtggtc
      301 gtccctcgat gcggacagcg aaggcgagga gggcaagttc tacgtgtgga cgcccgatga
      361 agtgcgcgct gcgctcggtc ccgtcaacgc gctcgtgttc tgccaggcgt acggcgtgac
      421 ggacgcgggc aacttcgagc acggggcgac ggtgctctcg cgcgtgacac cgcggtcgtc
      481 gccgcatgaa gaagaacagc tcgcggagct gcgcgcgcga ttgttcgagg cgagaggcaa
      541 gcgcgtccgc ccgggaaccg acgacaaagt gctctcgggc tggaatggtc tcgcgctgac
      601 ggggctctgc gcggcgtggc gcgcgacggg gcatgcacct gcactcgagc tcgcgaagcg
      661 ggtcggcggc ttcctgcgcg accacatgat tcgcgatggc cgcgtggctc gcgtgttcca
      721 cgaaggcaag acgaaggagc tcgacggcac gctcgacgat tacgcgttct gcgcgcaagg
      781 cctgctcgat ctcgcagaag ccacgggcga tggcgcgtgg tggaagcttg ccgaggggct
      841 gctggcgacg gtgcgcgaac gcttcgtcga ggaagacgcg ggcgtcgtcg tgttttacat
      901 gtcgcccgcg ggagactcgc tgctcgtgca ccgccccgag tcacatcacg acggagcgat
      961 tccgtcgggc gcgtcgatcg cggtgcaagc gctccttcga gtgggcctcg tcgccggcga
     1021 tgcagctgcg ctcgcgctcg cggagaaata cctggcgcag cggctcgcgt cgtcgacgca
     1081 ggtcaacgcg tggtcgacgt cctcgttgct cgcggcgctc gatctctatc tgcatgtcca
     1141 agagctcgtc gtcaccgatg gcactggccg cgatgcgctg ctcgcctctg cgcgtgaagc
     1201 gtacgcgccg acgctcgccg tgatcggacc gtgggcacaa gcgtcgatcc tcgacggcaa
     1261 gaccgccgac ggcgatcgct cgcgcgcgtt cgtgtgcaag ggcccgacgt gctcgccgcc
     1321 ggtcaccgag ccagccgagc tcgtgaagct gctcaattcc actggctgag ccggctcttt
     1381 gcagccgtca gcggcttcgt cgtcggcgtg ccgttcgctt cgcacgtcgc gagcgtctcg
     1441 tacggattct tcacggcagc gcggtagcca ttcaccgctt cggtctcgca caggtagacc
     1501 tggaactgac tctgcacgtc tgtcgcgcgc accgtcggat cgcccgcgat tgtcgatggc
     1561 agcgtcggga acgtcacgct ctgcgcgatc ggcccgaaca cgcgccacgt gtagatcgtg
     1621 ctgccgcgcg gcgagcccgt cgcgccggcg tagcgcaagt tcgcttcgaa caagtcgatc
     1681 gcaccggtgc cggtgacact gacgtcgatt gtcgtcgtcc ctggcgtgta cgtcgccgtc
     1741 acccacggaa gtagatttgc gccgacatcg agcgagtagc tcgcgagcgt gccgtcgacc
     1801 cgctcggtga tcgtctggct cacgctgccg atgttgttcg acaagcagtc gggtgcagcg
     1861 cccgtcgggc agcgtacgcg cgtcttcatg aatgccgtgg cgctcgacga ggtcgccatg
     1921 ttcatcgtca ttccggtgcc catcgcgatc ggtggggtgc ggatgtacgg cacgtagcgt
     1981 tccgcgacga tgtcggtgac gcgcgtcgtc ggattgctgt acgtgacggc cagcgtgtcc
     2041 atcgcatgcc acgagtccgt gatggtcgtg ttgcctcctg ccgtgaccgc gacgtcggtc
     2101 ttctgcgcgt agctgatcgg cgctccgcca ccaccacgtg cgaccacgac gacggtgctc
     2161 gtcgcgggcg tgcagcccgc gagcatgacg acctgcgggg cgagtgtcgt gccgacatcg
     2221 gtagcgccgc acgcggtgta gaccgtgtag ccctgtgcgc cggtcgctgc cgtgtagttg
     2281 accgtaaatg gatagccctt tgcggcggtg ccgagggtcg tcgcgttgcc caggccaccg
     2341 ccgccgagat tcgcggtgaa gctcgtcggt gtggcggtgg ctaccgtcca cgtgccgttg
     2401 tagccggcag gtgcagtgcc ctggatgacg accgtatcgc cggcagcgag accatgcggc
     2461 tgcactgtcg tgaatgtcgc ggtcgatccc gatttcgctg cgctcaccag atccgccgac
     2521 ggaagtggca gcacgcggac cgaaaacgga tcctcgccgc tcgatgccgc ttcccacgcg
     2581 gtgacgagct cgatgtcatc gccgggattg agcgcgagca gcgtcgtcat cgagtaggag
     2641 ttgccacccg ttcgctcgcg gatcgcggtc acgctcgcat tcggaaagac actcgcctgc
     2701 acggtgccgc tcgcgtcggt aaccgcctgc gtgaccgtcg cgtcggtatc gatgaacacc
     2761 acgtcgatgc cgctcaccgg tccgtcgagc ttgtcgacga catgcaccgt gaccatcccg
     2821 cgaacgttcg gatcggcatc gacgtcgatc gcgtcgtctc cgccgtcggg cgtcaacgcg
     2881 tcatcgccgc agccgccgca tcccgcaacg agcagaagtg cagcgacgag gaacctcacg
     2941 cgatcgagct taagcggatc ttctcatcgc atacatgagc aacagcacga acacgacgag
     3001 tgggaggagg taggggagca cgacaatccc gcccaacgcg aatcggtccc aacccacgat
     3061 gatttcgaac gccgcgaccg aggccgaaat cacgaacgca ccgatcgcct cgggccagtc
     3121 ggtttcgatc ttgagaccaa gccagaagat gagcgcaccg acgacaaacg ccaccgtcgc
     3181 gagcgcccat gcaggcagtc cgaggaacgt gtgcacgccg ttgcgagctg cgtcggggtt
     3241 cttccagacg aggttgacca cgatcgcgat cacgaccagg agcaccatca agatgaggta
     3301 cgtccacgcc ttggacttct gcccgcccat gaactcacgc tcttgaggtt ccgccatagg
     3361 cggccatcct atcggaaggc gcgggcgcgt tcgagcaacg gacgacgagt agctgcgagg
     3421 tgatcggcag ccgcgagcgg ctcgagcacg tcacgcacgg ccttccactc ggctttggcc
     3481 gttttcgcgt cgccggtggc tgcggcgtgc tctgcgagcc ccgcgtggat ctcggccagg
     3541 tgacccggcc agttcgcatt ctgcttcgcg gtgttcgccg catcggccgc gatgccgagg
     3601 gcttccatca gcagcgccct ccgctcggtc tcgtcgccga tcgccttcga gagcgtcgcc
     3661 gaagcgagta gcccttcggt cagggtgtct ttgaactctt gattgatcga gtcgccggcg
     3721 acgagcatgc gcgcacgatc gaggccgctc ttgatcagcg ctgtcgcgcg cttctgatcg
     3781 ctcgaggcga gcaggcgagc gaggcggact tcgctccacg ccagctcgtt cttgaagcca
     3841 ccttgggctg gcgactccgc gacgagctgc ttgcgcagtg cgaacgcctg ctcgtgagct
     3901 tcgagcgctt gcgccgtgtt gccttgagcg gtgaacgcgt ttgccagctt cgcgtaggag
     3961 aatgcgaccg agcggcgata tgcgacgttc ttcgtgtcgg catcgacgag gtcgctgcgg
     4021 atctcgagcg cgaggcggta cgcagcgagc gcgtcgggga acttgccaag gccgaggtgc
     4081 gcatcaccga cgcgcgtgtg agagcgcgac agcgcgatct tgtaacgcga gctcttcgtg
     4141 tcgcgcgcga cgagatcctt ctggacctcg atcgagtagc cgagctggac gagcgcgtcc
     4201 ttgtaggcgc ctgtgttcga caacgtgtag ccgaggtcgg acatcaagtt gccgcgctgg
     4261 tactgccact ccgtgttcgt cggatcgcga tggacgagcg cttcggcgag cgggatggct
     4321 tccttgtacg tctcgatcgc tgcgttctcg tcgccggtcg cgcgctggag atctgcgagc
     4381 tcgcgatcga cttcgaggac cgcagcttgc cgctcgacgt cgtcgggcgc ggcctcgagc
     4441 agcgtttggc gctggcgcag cgcgaggcgg tactcttcga gcgcggagcc cgactcgccg
     4501 cgattttgaa acaccgcgcc gagcttgaag tggctcgtcg agagagcgag cagctcgacg
     4561 gacggacgcc cgttgcccgt gctcgtcgcc gcttcgcgct cgttcttcgc ctcgttgtat
     4621 tgctcgaagg cttcgtcgat cttgccgtcg atgcgcaaga gatcgccgag gtgatcgtgc
     4681 gtttcggccg ctgcgagcag gatctccttg tcgttgagcg agcccctcgc gagctcgtcg
     4741 aacatcgctt cagcgcgctg caggtgcgtc tgcgcttcgg cgctcttgcc gcgctcctgg
     4801 aacacgcgcc cttcttggaa atcgaactgc gcgatcatga agcgcaggcg gcggctgtgc
     4861 tcggacttgt cattgccgac aactgcgatc aggtgctcgc gggcttgctt ccacgtctcg
     4921 agcgcttcgt ccggcttgcc cgacactcgc gacgacgcgc cgatcagatc gatcgcctgc
     4981 gccatgcgga tctcgtcctc gcgtgacatc ccgccgggcg ctcgctcgag gacggcgtag
     5041 tacgacttca cttcctggcc catgttcgac agcagatcga ggcgaccggc agcggagagc
     5101 cgcgtcttca tctcgtcgaa catgtagtcg atgaggtgct cggcggcctg cttgcgcgtc
     5161 accgcgattt gctcttcacg acgtgctcgg tcgcgcgcat cgacgatgtt gcggacagcg
     5221 atcgtgccgc cgaccgtcat ggccgtgatc gcgatcgccg aaatcgtgac ggcagcgcga
     5281 tgcttgcgga cgaagcgcgc gagccgctgc gacgccgtgt agcgatgcgc acccacgagc
     5341 tgcccggtga ggaagcgacg aagctcgtcg gcgagctcgc ccgcgtgcgg atagcgatcg
     5401 taggggctcg gcgccatcgc gcggtcgacg atcgcgacga gatccgcggg cgcttgcttc
     5461 tcgcgctcgc gaagcgggac gatcttgccg agcgccgctg cggcgatcac gtccgtcgcg
     5521 gtgcgcgcgt tgtacggcgg cacgccacac agcgtgtgat agagcatcgc gccgagcgaa
     5581 aacacatctg cgcgttgatc gagcggctca ccacgcgcct gctccggtgc catgtacgcg
     5641 ggcgtgccca tcaccgcgcc agcgaccgtt agcgtcgagc tgccgggcag cggcaccgtc
     5701 gtgacctcgt ccttcttgcg aacagcctgt ttcggcgagc ggttcgccga ttcgtgcgtg
     5761 tctgcggcat cgagatcctt cgcgaggccc cagtcgatca cgaccgtctc gccgaagtct
     5821 ccgacgagca cgttgccggg cttgagatcg cgatggatga tccgctggct gtgcgcgtac
     5881 gcgatcgcgt cacacgcagc cgcgagccgc gggatgatgg cgagtcgatc ttcgaggcgc
     5941 gtcgcctcac cgatgacttt gtcgagcggc cgccctgaca cgagcttcat cgcgaagaac
     6001 ggctcacccg ttggccagcg ccccgcttca tagacgggca cgatgcccgg gtgctgcagg
     6061 cgcgcggtga tcaatgcctc gcgctgaaac cgcgtgatgt gctcgccggc tgggtcgagc
     6121 agctgcttca gcgcgacgcg ccggtgcagg cgccgatcct cggcggcgac gatcttgccc
     6181 atgccgccgc gcgcgagctc gcgatcgatc tggtagtgga ccgtgctgac ctcgacgagc
     6241 tcgggctcgc tcgacgtctt atcgccgggc tcgcggctcg cgggtgcagc gaccgtcgcc
     6301 tcgagaccgc tgtcttcgat cagagtcccg ccggccagct tctccggtgg cgtcgggaca
     6361 tcggccggcg cgatcgtcgc ggcgaacgca gcggagctcg ctgcattcgg tggcggatcc
     6421 ccgtccttag acgccatcga tcatcgatag taaccgcgtg gagtcgtctc gaccacgtgt
     6481 gttgatgatt catggcagcg ccgcggaccg gacaacatgg tcgattcagc ttgccggacc
     6541 gttgcgcgag aaattcgagc tgaccgcgta cgaccgaggc gagctcccga gtgtcgaggc
     6601 ctatgccgcg gaggccgcca cgcggatcgg cgattcacga gcgctcgtcg ttggttccag
     6661 ctttggcgcg gtcgtcgcgc tcgagcttct gcgtacacag ccgcagctgt gtgcgggggc
     6721 gattctgatc gagccgccga tggcaccgac cgatgctccg cccaccgatc ccgtcgcgtt
     6781 cctccgcgat ttcgatctca tcgtgagcga gcaaggcggc cccgcagccg gcgagttttt
     6841 cttacgcacg gtgctcggcg atgccgcgtt cgagcgcatc ccgaaagcgt ttcgcgatcg
     6901 atccgcggcg aagtgggcgg cgattcgcgc cgactcgcag gcgctgatcg agtaccggcc
     6961 gcgctacgcc gagcttgcga ccgtcaccac gccggtcctg ctgctcggcg gcgaccgcag
     7021 cgcgccgtac tttcggccaa cgctcgatgc cctcgctcgc gtcctgcctt ccgcgaagct
     7081 cgagatcgtg agcggagccg ggcacatgct gcatgccgag gcgccacgca ggtttgcaga
     7141 gctgctgatc gcattcgccg ccgagatcca gatcgggtaa ggtccgcgcc cccatgacga
     7201 tagacggcgg aacattgttc gaccggctgg gcgaacgtcc gcccacggtg gtcgtcgcgg
     7261 tccagcttcc cggcgtgacc gaaggagagc tcgcgtcctc gatcgaagag ctcgaacggc
     7321 tcgcgaagac actcggcctc gatccagtcg cgcgcatcac gcaacgccgc gcgaagctcg
     7381 caccgggcgt cgtgctcggt gagggcaagc tcaaggagct cgccgagtgg accggcggca
     7441 ccggcgagat cgcgtcgttc gaatcgacgg tcgcgcgccg caagcgtcgc gaagacgaag
     7501 cgctcgaagc ggaagatgag gacgacgacg acgacgagac cgaggacgac gacagcgagc
     7561 cgggcaccaa gcccaccgcg aaagcgaagg tcgtgctcgt cgatcacgat ctgacaccgt
     7621 cgcagcagac gaatctcgag aaggcgaccg gcgtcgacgt cctcgatcgg acgagcgtca
     7681 tcctcgagat ctttcaccgc cacgcgaaga cgcgcgaagc acgcctgcaa gtcgagatcg
     7741 cgcggctcaa gtacctggcg ccacgcttgc gcgagagcgg cggcggcggc gatcgcgtac
     7801 gcggcggcat cggcggcaaa ggtgccggcg agacgtcgct cgagctcgat cgtcgacgca
     7861 ttcgtgatcg catcgcggag cttcgcgatg agctgtctcg gatcgaaggc ggcgcgaaaa
     7921 cgcggcgtga ccggcgcagc gagctgccca ccgtcgcgct cgtcggctac acgaacgccg
     7981 gtaagtcatc gatgatgcgt gcgctgacga gcacggacgt gtacatcgcc gacaagctgt
     8041 tcgcgacgct cgacaccacc gtgcgtcgtc tcgttccacc gacggagccg ccgatcctcg
     8101 tgaccgacac ggtcggcttc atcaagaagc tgccgcacga tctcgttgca tcgtttcgct
     8161 cgacgctcga cgaggcgaaa gaagccgact tgctactgca cctcgtcgac gcgtctgatc
     8221 cggcgcacgc cgatcagctg gcggtcacgc gcgaagtact cgccgagatc ggcgctgatg
     8281 aagtaccgac gtggctgttg ctcaacaaga tcgatcgcgt cgacgaagcg gagcgcgcga
     8341 agctcgcgga caagtatccc ggcgcgatcc agctgtcggc gaagcgtacg tcggacgtcg
     8401 gcgtcctgcg cgaccgtctc atcgaggagt tcatcggcgc gctcgaggag gccgagatcg
     8461 acgtgccgtg gtcgcatcaa cgcctcatcc acgtgatgca cgagacgacc accgtgctga
     8521 gcgagacgca ccacgacgat ggcacgcgcg ttcgcttgcg cgcgcccgcc ggcacgatcg
     8581 aagagctgcg caatcagctc aaagaggatg cacggtgaag acgccttcgc cgttgtaggt
     8641 cacgtcgtcc tcgggcaacc ggccgctgct ctcgccctcg acgacggacc cctcgttgac
     8701 gtagggattg atgtcgacac tcgagtcgca gtggaattcg agcgtcttga cggcgccggg
     8761 atgcgagatg tttgcgattt cgatgcgcgg cagcgtcgag ccgctcttgt tgcttttcac
     8821 gaacacgtga atctcgtcga cgaatgacca gtcgtccatg tcggtgccgg tcatcgcggt
     8881 cgccgtgatc gagagcgtca acttcgacag cgtcacgcta tcgatggggc cggtctccat
     8941 cgctttgatc ttggagctga gatcgatcga cagcggcagt gggaacaaga cggcgagagg
     9001 gccggggagg ggagagcccg tcacgcgttg ctccggcacc ggctcctcga tgtcgaagtt
     9061 ggctacgcca catcccgcga ggagagctac aaggcaaacg aatcgcacga gctgatcgta
     9121 cgcctgccgc tgcgctgtcc aaatgagggc ggagttaggg ccgccactcg tcggcgccgc
     9181 gatcacagag cagatacggg cgtgcctgcc cgtcgatatc gtcgagataa gctgcggaat
     9241 ccgtggcggt cacccggcaa tcgtaggcag gggaggtcga acggtctgcg tcgggatcat
     9301 cacggatcac cggattgctc gtcggggaca tcatcgtcag gtggaagtcc gtggcgctcg
     9361 cgaacttgat ctcggcgaac tcgttgctgg tgccgacgta gttcgagaag tagtcgcagt
     9421 cgccgccgac ctccgcattg gcagcgccgt tactcgtgaa gatgttgtgg gcgacgagta
     9481 ggtcggggct gcctccacac ttgagcgcac cgaaccggtc gccgccgccg ttctcggagt
     9541 tgttcttcgc gacggtgttg aagacgatgc gccccttcgc cttgtcggtg cggatatttc
     9601 cctgggtgag cgcgctgctg ccgttgtcgg ggccgacgaa gttgttgcgg atctcgaacg
     9661 tgccgtcggc gatgaacatg ccgccggcct ggttgcgcat gagcttcgtc cgctcgatcg
     9721 ttgcgtcgca cttcgtcgtc tgtacgccgt agccgccgct gccgatgatc gtcgcgcgcc
     9781 gcagctggaa cttggtctcg ctgcacagga ttccctgcgc ggttgccccc gagatcgtga
     9841 ggcccacgat ctcgacgttc gtctgcttcg tcatcgagat ggccggtccc gcgcccatcg
     9901 ggatgaaggt cgcgcccgtc ccgatgatcg ttcccgacgc agcgacgacg atcggcggct
     9961 ccgagtagtc ggtgcccttc gtcgtcgtga gcttgacgat cgactttgcg ccgagcagcg
    10021 acacagcggt cgtgaacgtg cacggcatgt cgatcgtgca gagattgccg gtaccgttcg
    10081 gtgcgggcgc ggcgtacagc acgttgctct gcatcgcgca gctgccgtcg ctgaggcaga
    10141 ccttcgagtc cttgcagtcg gtgtcgacga cgcacgcgtg gcacttgcga tcttggccgc
    10201 aggtttcgtc ggccgcacat tgcattggat cgctgcccat catgcccggc ggcaagcact
    10261 ggacgcagtt gccgctcgac acctcgcaca cgggctgctt ctgatccgtg cacgcgatca
    10321 tcggacaccc aacgggcgca tcgaccatca cgagatcagc cgcacggcac tcgacatcgt
    10381 tggggttcgc cgcgcagtac tccgggttga gctgcctgcc gcagccgacc gcgaggagcg
    10441 caagcaagat cgcgcgactc atcacgacga ggatcgcgcg gatcgcggcc ggtgtcagct
    10501 cagaaacggg tgacccaccc gacatatgcc tgaccaggtc ccaccgacgc gatcggcgcg
    10561 gattgccgcg cgttagcttg cttcagcaga atgatgccac caatcgcggc ccccactccc
    10621 acagcggtga acgcgatgcc ggggaggatg gcatcgggat acgtgtactt gtggtccctg
    10681 tcgtggagcg cggtgccgta cgtgccgtag aacaggaaca ctccgcccgt accaatggcc
    10741 gcgatgccgc tgccaaggag cagcattggc cacactttcg aagctttcgg ccgtttggtc
    10801 accggcaagt cggcgtgcat cgcctgcagc gtgacgagct cgtcgtcgac gatcttctcg
    10861 aacgttgcgt cgcaatcggg acacggcacg ctccggccgc tcgtgggttg ctccttcgtg
    10921 aaccagtaga ccttcagcga cggcttggtc ttcgcgtcgg gatcgcccgg atcgaagccg
    10981 atatagatga gggtcgcgct gcgagagcga ttgtcgaaca cgccgcgcgc gcatttcatg
    11041 tcctcgagga caaagcagtt gtcgaaggaa ttcgccgcgt cctcggacat cggcgccttc
    11101 acgagcatgt agcccttgct cttgacccac gcgcgggcat gctcgcgcag cttgccgtgt
    11161 agcggcgtgt cgccaacgac gagcacgccg acgtccggtg tttctgcgcg caccgccgga
    11221 gcagcgagca acaccgcgag aatcacgacg acgcgagtca tcccctcgcg gcatcgtagc
    11281 gcggtttcta ctgggcttcg tcggcgccga tgtcttttgc accgacgcga ggctcgccgt
    11341 cgatatccgt gctcatcgcc gcgcccggct ctgcatgatc gactgcatcg ctcgacgctt
    11401 ggatgcggaa gtaatcggcc gcgagcgggt ttgtcgcgtc ggtcgctttg aacttcggat
    11461 cgccgctgat gttggtgccc gtcggcgacg cgagtagcga gtagtcgaac atgcagtacg
    11521 tcgaggcgag gttgccgctc acgatcgtgc tgctgatcgt catcgggatc gcgcagttga
    11581 tgccgcgcag cgcgagcgtc gagctgctcg agacgttgtc cgcgaccgta ttgaagcgaa
    11641 atgcgttctg gctacccgac ggcgtcatcg tgagaccacc ggtcgtcgac gtgctcgctc
    11701 cgttcgcgac gaacagcgag ttggtgatgt cgatcgcggc catgatgatc gcgccgccgg
    11761 tcttgtttcc cgacaccgtg cagctcgtca ttttcagcgt tccgccgagg acgttcacgc
    11821 cgacgcccga gttgtcgatc accgagacgt ggtcgagcgc gagcttcacg gcctcgccgg
    11881 agggaacgcg aatgccgtcg ccggtgttac cgagaccatc gcgaatgatg aggccctcga
    11941 tcgcgacgtt gctcgtgccc tgcagcgtta gcacgggacc cgacgacgcc gttcgcgtca
    12001 gcgtcgtgcc tgcgtccgcg aggatgtgca cgttcgcgcc gctcagcgac accgcctcgt
    12061 cgaagctacc ggcgagcttc acgtacggct tgccggttgc gaccgcggcg ccgatcgtgc
    12121 ggcacggctg ttcgttcgtg cacggatttg cgtcattgcc cgccgtcgac gcgtacgcga
    12181 ccgtcacgtc ggagccacac gcgccgctcg gtagacatgc tgccgacacg cactcgctgt
    12241 ggagctcgca tgcgcgacac gcgtgatcgt caccgcatac cggcgagctc tcactcgtgc
    12301 attccgtcga cgccgtgcat cccgtcgaca ccggcgcgtc cggcgtgtcc gtcgccatgc
    12361 agttatggtc cggtgcgttc tcgcagtaat acggattgtc tttgctgcac gcagcgagcg
    12421 cgagcgtcgc gaagaggatg cgcttcatta gaatctcccc gtccagccga cgacggctcc
    12481 gtcatgcgtg acgctcgcga cgggcgcgcc gtgcttgcga tcttgcagcc acaagtagac
    12541 gccggtgccg atcatcgcgg ctcccgcaaa cccgatcacg aagccggcgg tgcctgaatc
    12601 gcgatagctc ggctgctgcg gccccgtcgg gctcggatct tcgtcgatcg cgacgagcac
    12661 gccgccggtc aagagcgcag cggcgcctgc tgcagcgaga ccgatcggca gcatattgcg
    12721 gcgcggttgg ccttcactca ccggcatcac cggcgtcgtt tcttcgaccg gtggcgctgg
    12781 cggcggcgcg gactccgccg gaagcggcgg ctggtgaacg agcgcgagca tcagctcgtc
    12841 gatcgtggtg tgcagcttct cgtcggtgca cttgtcgcag aggcggcgct ccgacatcga
    12901 ctcgtgattc ttctggaacc atgtgccgac cacttcgacc tggcgcgtgc cgtcgtcgcc
    12961 ttgggttgca tcgatacgcg cgtagatcac cgacgtcgct ttgctccgcg catcgatgag
    13021 gccgcgcgcg cagccgatgt cctcgaggac aaagcaatcg atcaacgtgt tgaccgcgct
    13081 cgggtcgaga gccgcgggtg cgatcgtccg cccgcgtgcg tgcagccata cttcgacctc
    13141 ggatgcgacc tgcggctgca acgtcgcttc tccggtgacg acgacgccca cttgttcggc
    13201 gtgtgctggc gtcgcgatcg ccagcgcgag gaccacgaaa gccgtgcgca ttgtcagttc
    13261 cgatcgatgg tggtcggctt cgactcgtcg atcgaaacac tcagctgctc ggtctttccg
    13321 gtttcggaat tgaccagctt cactttgtgt tttccaaccg agagcttcat gtcgaccggg
    13381 gtgtcgtgga cctttcggtc gtcgacccac accgtctgaa cagggaacgc gtggacgacg
    13441 agccggccgg tccgtttctc tttgccagcc tggcccgtct gcgtcgaagt tgcatctttt
    13501 tgaccggcgt cgaccggcgc tgccgcgacc tcgacggctg catcgggggc cgccgcgtcg
    13561 ggctgcacgg tcgcgaccgc cacgtccgcg gggacagcca ccgatccggc ggccgatccg
    13621 tccccgcttc ccgccgcaac cacaggctcc ttgccggact gggtcgaagc gacaatcccg
    13681 acgacaacga acacggcgac gaccgcgccg acgacgataa tggcgacctt cttgccgcca
    13741 ccgcgccgcg ggatgaccgc gctcggctgg atcgtgccag ttggcgtact gcgtgccgca
    13801 ccaatgctgg gcatcggcgt cgcttgcccg acgtaacccg cgatcgtctg ctgatcgtgg
    13861 ttggcttgga tgtagccttg tggcgtctgc gtcgacgcgt acccgagcgc cgggccacct
    13921 tgctgcggcg cctgaccttg cgggggcgtg cctccgcggc gagccacgct ctggcgaacg
    13981 ggggcttggc cgggaaaccg ctcggccatc gtcctcatca gcagctcgcg gccggccttg
    14041 ggcgcgtcgc tgcactcgaa cagatcgtgg atggcgtcct cggcggtcgc ataacgcgcg
    14101 ggcaggtcgc gctcgaggag cttcatgacg acccgctcga gatccttcgg gatgtcgctg
    14161 cgcatcgagc gcggccgcgg aatctgcccg aacaacaccg ctgcgagcgt cgcgcgcgtg
    14221 tcctcgccga cgaacagccg ccggccacac agcatctccc agagcatcac gccgaccgcg
    14281 aacaggtcgc tgcgcccgtc gagcgactgc ccgttcgcct gctcggggct catatacgcg
    14341 ggcttgccct tgatgaagac cgatgcggtc gcctcgctcg cctgcttcgc tttcgcgatg
    14401 ccgaagtcgc tgaccttcac cgcgccatcc cacgacagca gcacgttgtg cggcgataca
    14461 tcgcgatgca cgatgccgcg catacccgcg ccaacgggca agtcatgcgc gtgaccgaga
    14521 ccgcgcagca cctcggcgat cacgtagatg atgaccggca gcggcagcgg tccactcgac
    14581 gcgagcgcat cgaggtcttt gccctcgacg agctccatga cgaggaacaa gcgcccctcg
    14641 ggatcgcgat cgaaatcgag gaccgagacg atgttcgcgt gctgcagctg cgagctgatc
    14701 tgcgcctcgc cgatgaacat ctgcgagaac gctggattct ccgagaagcc cggcaacacg
    14761 cgcttgatcg cgacgcgccg ctgaaagcct tcggcaccga gcatcgagcc gaggaagacc
    14821 tcggccatac cgccgccgcc gaggcgctga tccaggcggt atttgccggt ttggccgagc
    14881 accgaggctc tcgattatac gcgagggcag gtgcaacaaa cggttgcacc tcctaggtat
    14941 cgagacccac cagctcacgc gcgtcgtcgc agtcgaggtg gacctctcga tagaggccat
    15001 tggccgcctc gaggaggaac gcgtgacgtt gcgccgcggg ggcctcgttc gctttgcgaa
    15061 tcgcatcgcg caccgacgca gggacatcct cttcgaccac gagcaggggt aggagcgaca
    15121 gactcatagc ggcgaccttt ctgtgggctt gcgcaaaagc gtggctgcga gcgtgagccc
    15181 gaccagcaca ccgacgagcg cgaccgaata agcgaagcga tatccgagca ccgttgcatc
    15241 gtgcagtgag atgcctgcgt cgacgagaac gcggctgcgg tacgacgccg cagtgacaag
    15301 cagcgcgacg ttgacggcgg ccgccatctg catcgacagg ttcacgatcg ccgagccaag
    15361 ccccgcatcg ggcgcgggaa cgtcggccat cgcgagtgtg agcagcggca gaaacgacag
    15421 gccgaagcca aagccgagga gcacgtgcgc gatcgcgcgc agcgggaagt acggctcgtg
    15481 gatggcgagc gcggccatga tgccgaggcc gatcgctgcg atgccgaggc cggcgacgag
    15541 cacgcgcacg gcaccgaagc ggcgaacgaa ccacgcgcta ccgccgagcg acagcgacgc
    15601 gaccgtgagc gtctgcggga gaaatgcgag cccgaccttc agcggcccga agtgcagcgt
    15661 gttcgacata tcgagcgtcg cgaggaagaa cacgccgtag aggcccatga ccatgaagcc
    15721 gcggacgacg ctcgtcacga cgaggctgcg aatgcgcagc atgcggatcg gcagcagcgg
    15781 gtttggccgc atcgcttcga ccacgaagaa gacaacgccg agcacgagcg cgagcgcgag
    15841 cgacacgcgg acggcgggcg cagtccacga cgtgtgtccg gcttcgacga ggccgtagat
    15901 cgcgaccatc gccgccgatg tcacgagcac ggcgcccgcg acgtcgatcg gtgcgcggct
    15961 cttggggcgc gccgcctcgc gaaggttgcg gtggacgagc agcgcggcga tcgcgccgac
    16021 cggcacgttg atccagaaga tccagtgcca gctcagcacc tgcgtgagca ggccaccgac
    16081 gaacaggccg agcgagccac ccgagacgct gacgaacatg tagccgctca tcgcctttgc
    16141 gcgatcggcg ggcgagggga actcggtggc gataatcgcc aggatgacgg aggacgcagc
    16201 cgctgcgccg atgccttgga tgaagcgcgc gatgatcagc gtggtggcgc tcggcgcgag
    16261 accgcacgcg atcgacgcca ccgtgaacgc cccgatgccg agcaagaaga tccgcttgcg
    16321 accgatcgag tccgagagcc gcccgaacag cagcagcagg ccgccgaacg cgatcaggta
    16381 ggcactgttg atccaggtga gcgatcccgg cgatagcgcc aggtcctgct ggatggtcgg
    16441 catcgccacg gcgacgacag tggcgtcgag gacgagcatg aactgcccga ggaggactgc
    16501 gacgagcccg atgatacgcg acggaatctt atgatcactc gttattgtcg cgtccatcat
    16561 gatagtcaca atagttgtga ttatcagggg tgtcaatacc gtaaagaatg gttatagtga
    16621 cttcgtggcc agctctcgta acgtcccgca catcctcaac ctctggaggc gcgttgcttc
    16681 ccgagcgacg gaggagtcga tgggcatgaa cctcaagcag gtcaccatgc tcgcgaacct
    16741 gcgcgacaag ggcgagcttc cgcagaccac gctctgcgac atgatgaagc tgacgcagaa
    16801 cacggtcgtc acgtggctca acgagctcga ggacgccggc tacgtcgcgc gcattcgcga
    16861 tcccgaggat cggcgcaagc acaacgtcgc gctcaccgac aaaggtgcag gcgcactcga
    16921 gcgcgcggag accgagctgc gccgcatgga agacgaagcg ctcagcgggc tcacggccga
    16981 cgaacggacg cagctgcgca agctcatggc caaagcgctc gaagccgtcg actagatttc
    17041 gctagagctc gtctagagct cgaaaaaacc gtgcatgcga gcgacgcctc gctctgcgaa
    17101 gaaccggcgc gccgcttcga tgtgcgtcgt gaacgcgaac acctcttcga gaccagccgg
    17161 tcccagaatc acgccgcgtg cgctcgtctc gctgtcgggc gcaaacggcg gtagcgcatc
    17221 ggcacgaagc tcttgcgcga cggcgaacag catcacgcga ttcggcttgg gctcgctcga
    17281 cgcgtaccaa aacggtacga gcttgctcgc atcgatctcg atccccgtct cttcacgcac
    17341 ctcacgcgcg cagcccgttt gccagctctc gtgctcctcg agaaagccgc cgacgagcgc
    17401 gagcatgccg atcgccggag gaatcgcgcg tcggatcgtg agcaaccccg tgccctcgct
    17461 cgtcacgatc gggaccagcg cgacacacac gggaatcgga ttggcccaga cctgcgtctt
    17521 gcagcccggg cacgtgcgcg ggtacgtcag cacgtcgtac ttcgtgccgc agaagctgca
    17581 gaacgtgtcg cgcgcgaggc tcatcgtggg atcagcgaat cgtggccgcg ctcgcgcatc
    17641 tgcaggcgcc aggcttcaat cagcttcacg aacgcatccg agagctcggg gtcgaactgc
    17701 gtacccgcac agcgctcgat ctcaccgatc gccacctcgt gcgcaagggc gcggcgatac
    17761 gcgcggtcag acgtcatcgc gtcgtatgcg tccgcgatcg agacgatgcg gccgacgagc
    17821 gggatgttct ggccactgag gccgcgcgga tacccgccgc cgtcgaacca ttcgtggtgg
    17881 caccagcagc catcgatgag gccatgcagg cacggcaccg gctcgaggat gcgcttgccc
    17941 ttctcgggat gctggcgaaa caccgcgatc tcttccggcg tcagcttgcc gggcttgttg
    18001 agcatgtcgt agcgaacgcc gatcttgccg acgtcgtgca tgacgcctgc ttgcacgatg
    18061 cggcgaatct ccgaatcggg cagcgtgaga ccgcgcgcga gcacttcgga gtagaccgca
    18121 acgcgctcgg agtgaccgcg cgtgtacatg tcgctctctt cgagcgcctg cgcgaagccc
    18181 gcgacggtct gctggaacat ctcttcgagc gataggttcg cgcgctcgag cgacgtgttc
    18241 ttctctttga tctcgccgta gagccgcgcg ttgtcgatcg cgctggctgc acgtgacgcg
    18301 agcaccgcga gcatcttgcg gtgaccttcg tcgaacttct tgccgctcgt gaacgagaag
    18361 acgttgagca cgccgaccat gtcgccgcgc accgtgagcg gcaccgcgac gaacgatgcg
    18421 aggtcggcag gagcggcgct ctcggtgaag aagcgtgatg ccttgccgcc atgcgcgagg
    18481 atcggcgtac ctgccgagaa ctgatcggtg aggatgccga atgcaggcga gggcagggat
    18541 gcatgcggct gcgcgtcggc ggcctcgacc ttcacgcgct cttcgtagtg accggtacgc
    18601 ggatcgcgaa ggtgcagcgt cgcgacgtcg gctttcacct cgtcgatcgc cgcgcgcaga
    18661 acgacgtcga ggatccgctc gatatcgtgc gagagcgcga tcgcttcgct gaccttgtag
    18721 atcgtgagcg cttcgcgcag gcgaatgttc tcggcctgca ggcgctggcg atagagcgcg
    18781 cgctcgacga cgtggatgac ctcttcgacc ttgaacggct tgagcaggta atcgtaggcg
    18841 cccttcttca tcgcctcgat cgccgtctcg acggtgccaa agcccgtcat gaggacggtc
    18901 aacacgccca gccgctcggc ctcgaccttc tccaagagct gcagcccaga cagccgcggc
    18961 atcttgagat ccgtgatcaa tagatcgtac ggatggagcc gcagctccgt cagcgccttt
    19021 tcaccgtcct cgacggtgtt gaccgagaat ccctcgagcg tcaaaaactc cgcgaggatt
    19081 tcacggatga cgcgctcgtc gtcgacgacg aggatccgag ccgcttccga ctgatcggtc
    19141 gtggccacga attcttactg ctgcggtgcc gtaccgccgc cgccgccacc gtcagtgccg
    19201 ccaccaccgc cctgttgcgg ctgcggcgtc gggttggaat tgatcggctg ctgctggccg
    19261 gggtctttct tctcttgcga catgccgccg ccggagagcg tcgggctgcc ttccttctgc
    19321 gccatcggat cgtagaggtt gccggtgcgg cccgtctgct tgtccgccgc ttgctgcgcc
    19381 gacagccagc tcgaacgcgc cgagaccgcc ttggtcttga actgctcggc cttgtccttc
    19441 gacgacgtcg tgcgcttgcc gcgcgcctcg agctgcttcg ggaagtcgtc gtagttgacg
    19501 cccttcggcg cgatgttgtt ctgcttcgcg agcgtcgcct tcgcgctctc gaactgcgcc
    19561 tcacgccagt acatgttctc gagcgtgtag cggtggtagc gcttcaggta gtcgcggtac
    19621 gcctcgaagt acttcacgcg cagcttcgcg gcctcggccg aatcttcggc ggccttgaga
    19681 tccttttgcg cgttgttcat gcggttcatg tccgccgact gctccgcgct cttcttcgcg
    19741 gtgatcgccg aatcgacggc gagcttggcg gccgagtagt cgttcttcgc ctggcggagc
    19801 tggccatcgg cctcttgctc gttcgcctcg gcgttcgcat tttccatctt cgcgatcgaa
    19861 tactggttct gcgtctcgac gacgccgccc ttctggtcga gcggaatgcg ggcgatgtac
    19921 atgtcatcga agtgcttcgc gagtggtccg ggaggcccgt cgccgcctcc gccacacgcc
    19981 gcgacaaaca aaccgacaaa aacgagcttc gctagtcccc gcataggtgg gcacagccta
    20041 ccaccaggac agagcggcga gctacagtca gcgcggaatg acgacgctgc ttgccgtcga
    20101 cgtgggaaac acgaacaccg tcctcggggt ctttcgggac aatgagctgg cagcgcactg
    20161 gcgcattcag accgtggcgc agcgcacgtc ggacgagtac gcggtcttgc tgaagacgct
    20221 cctcgacctc gacggctttc cgtggcgaag catcgacgcg gggatcatct cgtcggtggt
    20281 gccgccggcg gtgttcgggc tgcagcggtt cttcaagcag cactgcggcg gacccgccct
    20341 cgtcgtcgga cccggcatca agacgggcat gccgatcctc tacgagaatc cacgcgaggt
    20401 cggcgccgat cgcatcgtca acgcggtcgc agcattcgaa gagctgagga gcggctgcat
    20461 cgtcgtcgat ttcgggacgg cgaccacgtg ggacgtcgtc aaccccaaag gcgagtacct
    20521 cggtggcgtg atcgcgccgg gcattcagat ttcatcggaa gcgctgtacg agcatgcggc
    20581 gaagctgccg cgcgtcgagc tcgcgcgtcc tggcaaagtc gtcgcgcgaa acacgatcgc
    20641 atcgatgcag gccggtctcg tctacggcta cgcgggcatg gtcgacgcgc tcgtcgagcg
    20701 gattcgcgcc gaggtcgact ggccggcccg ctgcatcgcg acgggcgggc tcgctccact
    20761 catcgcaacc gagacgaaga cgatcgaggc gaccgacgag ttgctgacgc tcaaaggcct
    20821 caagatcctg ttccaacgca atcaggcgcc gggctgagcg ggataacgat ggagcacaca
    20881 ccactcgata cgtcgaacct ctcaccgggc gcgcagaagg cgctcggtcc gggccctgcg
    20941 aagatgatgg ccgcgcgcgg catggcgccg ttgccgccgg ccgagcaagc atcggtcctg
    21001 tatcagctcg cgctcgacgc tgacccgaag atcgccgaga gcgcgcgtca aaccgcagcg
    21061 ggtttgcccg acagcattct cgccggtgcg ctcgggaatc aacagctcga cgcgcgcgtg
    21121 ctcgatttct tcgccctgat cgccgccgac aagcctgccg cgttcgagac gttcattcaa
    21181 aacgccgcgg tcgcggacga gaccgtctcg atgctcgctg gcaaagtcgg cgcgcgagag
    21241 gtcgatcgca tcgccgcgaa cgagcagcgc atgctgcggc atccgcagat catcggcgcg
    21301 atgtatctca acaagcatgc gcgaatgtcg acggtcgacc gggcgatcga gctcgcggtg
    21361 cgcaacaacg tccgcgtgcc gggcctgcac gcctgggatg aaatcgcgcg gtcgttgcag
    21421 ggccaccagt cgaccgccga atcggatgcg ctgttcgcaa tggcggccga tggaatcgaa
    21481 cacgatgacc gcgtgctcac cgagggtgac gcagaaaagc cgatcgagga catcgagcgc
    21541 ctcgagcagc tcgctctcga acgcacgaag gacatcccga tccagaacat gaccgtgccg
    21601 gcaaaggtcc gcctggcgac gctcggcaac gcgtttcagc gcgccgtgct gatccgtgac
    21661 ccgatgaaga tcgtgcagat ggctgcaatc aaatcccctg gcgtcacgga gttcgaggcc
    21721 gcgagttacg ctggtaacca gacgcttggc gaagacgtaa tcagatacat tgcaagcaag
    21781 cgcgagtgga ccaagctgta cggaaccaag tactcgctgt gccgaaaccc gaagactcca
    21841 gtgaccgacg cgatgcggct gatgccgttt cttcgcgaga aggatctgca caccctgatg
    21901 aagagcaagg gcgtgccgtc ggcggtggta gcgcaggcgc ggaagctggt gatgcagcgg
    21961 cgcggcggca acgaccagaa caagaagtag agtcgtcgct gctcacgcgc gccggtctcg
    22021 cgatcacgca gccacgcagc gcgctcgcga tcgcaggcga tcgacgccac gccggacgct
    22081 ccggctcgga tctgctcgcg tgcatcctcg tcgttctcct cgcgacgcag ctgcgtgcgc
    22141 tcgtcggtgc gatctggctc ggcacggaag tgtcgggaac gctcggccta cgcgcggcgg
    22201 tgcaggtgct gaccgacacg ctcgtcgtga cgcttggctt tctcgtcgtc ggcgccgcgg
    22261 tgatcttcgt cggtagcggc ggacgccgcg agctcggccg cgcgttcgat gtcgcgtgcg
    22321 tcgcggtact gccgctgctg ctcgtcgatc tcgcaggctc cgcaatcctg acggcgttcg
    22381 atctcgagct cgagcgctcc gcgatgctcg tcgtaacagg gctcgcgtat gcgtggacga
    22441 tcgggctcat cgtgctcgcc gtcatcgacg cgcgcagtcg tcaagcgatc gacaacgctg
    22501 ctccgacgag cgcgaagcgc gcgggatggg cggtcggctt gctcgcgttc gcaggcatcg
    22561 ctgcgcaagg catctggctc gtcacccatc tcgagcgcgt gcggccgatg acgcaaggtg
    22621 atccagcgcc gcagctcgtg cttccgcata tcggcgcaaa cggcgacttc acgactgcgt
    22681 tcgatctcgc gcagctgaag ggcaaggtcg tcatcctcga tttctgggcg acgtggtgca
    22741 acccgtgcct gaagtcgatg ccgcatctcg atcagctaca gcgccggcat cctgagattg
    22801 ccgtcgtcgc gatcaacatc gatgaccccg caggcgcacg cgcgctgttc gacgaacggc
    22861 gctactcgat gcagctcgtc ttcggtgatc aacccgcgca ggatcgctac ggagtgtcag
    22921 cgatccctca cacggtcgtg atcgatcgct cgggagtcgt gcgcagcgta tttcgaggcg
    22981 gtggcgcgga tctggaacgc gcgattgcac cgttattgaa gtaggtggag atccggaagt
    23041 ggatatgtat ctccgcctct ggtaacagag acccatggcc agcctggttt gcgctacctg
    23101 tcgcaaactg attccgcccg gaacgaatgc gatccgttgc acggtcgcgt cctgcaatac
    23161 gggccggatg aagctgcgct tttgctcggt cggatgctgg gaaaagcacg ttccgacggc
    23221 gcgccatcgc aaggcggctt acgtcgtcga gggcgcaaat aaccgagaaa ccgaacccga
    23281 gtcgtaaaac gctcgtggcc gctcgccatt ttgcgaggga aatcgcgcac gtgttatttt
    23341 tcggcgcgtg cgtttgggga cctgtatcgc gctggagctg gccgtggccg caggcgcgat
    23401 cggcgggctg tggatcgggg tagggcacac cgttcgactc gcggacgagt acgtggcgga
    23461 gcgcgatgcg tctgctgcga cgctctctga tcgccatact cgtctcgcac caacacgcgt
    23521 gccgggcggt tggctcgcgg tccagcccaa gtccgcgccg gcgaccgtct tcgatgcgcc
    23581 tgatgacgcg ttgctcgcgc cgctcgccgc tgcgccggtc acgcgggtca agatcaaccg
    23641 cggtggcaca tcactgtcac tgcgcatcga tttcgcgaat ggctcgcgct gcgcgttcaa
    23701 gcccgagcaa atccatctgc attcgaatcc gcgcaaagag atcgccgcgt accggatcga
    23761 tcgcttgctg cagatcggac acgtccaacc ggccaaagcg atccggcttc ggctcgccga
    23821 cctcgtcgcc gctgccgatc cagacgtgcg cgccgtgacg gcgcagcgcc tgcacgacga
    23881 agccatcgcg cgtcccgtcg atccggcgga tccagccacg ctcgagctcc gcggcgtcgc
    23941 gtcgtggtgg atcaccgaca ttcgcgacgc gcatctcggg ccagctctcg tcgatcaagc
    24001 agagggcatg gacctgtgga cgcaagagct gaaggtcggc gcgccgattc cagcgcaaca
    24061 ccgcgtgctc gtcgagcaac tctcgacgct cgtcgtcttc gacgtcctca tcgacaactt
    24121 cgatcggtgg agcggctcga atacgaaggc atcgcccgac ggcaagatcc tctacttcat
    24181 ggataacgcg atcgcgttct cgaagtacac gcagggccac gacatgaacc tcgggccctt
    24241 ccgccggatc caaaagttct cgcgcggcct cgtcgagcgg ttgcgtgcat tgaataccga
    24301 gtcggtgacg gcggcgctcg atgtcacggg ccaggatgcg ggcctcggcc cgttgctgga
    24361 tgccgaccag atcgccgcca tcctcgcgcg gcgcgattac atcgtcggtt acgtcgaccg
    24421 cctcatcgcg cagtacggag aggatgcggt gctcgcgttc ccatgacgcc ctgtcctgtc
    24481 tgcggcaagc ccgtcgatcc gttgcgcgcg cccgcggtga gcgttcgcga cggcaaggtc
    24541 gtggcgttct gcagcaagga atgccgcgcg caagccgaaa cgcagccggt cagaatccag
    24601 ccgacaaaag tcgtgcggct tccttccgaa gacgcgccga tcgtcgggcg aactgcgacg
    24661 gccgacaagc tgcgcactcc gtcgacgggc gtgccgtcga gcggcgtccc gaaatcgacc
    24721 gcggatctcg acagtggccc ggtcatcgag atcgtgcgcg agccttcgcg acccgtcgtc
    24781 gtcgagccga caaagaagcg caagcgctcc gacagctcga tccagatcgc cgacaccggc
    24841 cgcctcgatg actacatcgt cactgatggc ggtggccgcg gtcgcatcct attggcggtc
    24901 ttcctgctgg gcctgctcgt cggcggcgga atcgcggcgt acaagctcgg ctacctcgac
    24961 ggtctcctga acaagaagcc ggcgcgcgcc gcaccgggag acgccgccgt cgcgcccgtc
    25021 ttcgatgacg ctccgcctgc acccgcgatc acgcccgacg ccgcgctcgc caaagccaag
    25081 tccgtcctcg aagcgcagct caagagcgac tcgccgcgcg ttcaacgcgt cgccgcttcc
    25141 gcgctcgctc gcacgggtga caaagcagcg ttcgatctcc tcgccaagtc gatcgcgacc
    25201 gaatcgagcg acgtcgcgaa gctcgacatg gcgtatgcgc tcgcgcgcag cggcgacaaa
    25261 ttcggcacgg acgcgctcgt cgtgatgctc ggcagcggca agcgcgaccc caagctcgaa
    25321 gcaggacggc ggctcgcgct cctcggcgac aagcgcgcgg tcaacgcgct cggcagctac
    25381 ctctccgtcg atcaactgcg tctcggcacc gccgagcaac tcgcgtacct cgcggatcca
    25441 aaggcgcttc ccgacgataa agcgcgcgcg acgatcgccc tcggtcacgc cggccgcacc
    25501 gatgtcgccc ctgcgctgca cgagctgctc acggacgacc gccaaaacgc attcgccgcg
    25561 caagcgctcg cgaacctcca cgacgaagcg gcgcgcccgg tcctcgtctc ccagctcggt
    25621 attccctcgc tgcgcgtgaa gagtgcgcgt gcgcttcgcc gcctggcccc cgacgccgac
    25681 gtcagcgcac tcttgccgcc gctcgtcgcc gcgctcgatt cgaacaagga cacggagcag
    25741 gtccagatcg ccgaagcggt gctgctcctc gccggtcccg cggcgtggtc ggagcacgaa
    25801 tgagccgcac gctgcgcctc ttctatctgt tccgcctgct tgcgacgtcc tatctctacg
    25861 tcccgatctt catgctcttc caggcccagc gcggcctgac gttcttcgag cagctcgcgc
    25921 tcggcggcat ctacagcgcg gtcgtgatcg cggtcgagat tccgaccggc gtgttcgcgg
    25981 accgcatcgg ccgccgcctt tcgatgatgg cgggcgcgct gatgatgtca gcctcgtgcc
    26041 tcgtcgccgc gagcgcgcac ggcttcggca cgttcgtcgt cgcggaatgc ctcgccgcga
    26101 tctcgatggc gctctgctcg ggcgcggact ccgcctatct cttcgatctt ctgctcgagc
    26161 acgatcgcgt gcacgaatat ccgcgtcgcg aatccgctgc cagcgcgtgg catctcatgg
    26221 gcagcgcatg cgcatttgcc ggtggcggcc tcctcgcgcg cgtcgatctc gcattgcctt
    26281 atctcgtgac cgcgggcgtc gcggcagtcg cggccgccgt cgcgttcatg ctcgacgagg
    26341 atcgacctcg cgaccgcgca cgcgcgcctg cgatgcgcgt gatgaggagc tggagccgtg
    26401 agatgaccgc agcgctcggc gacgtcgcgc gcaatggtcg cctcgcgtgg ctcatcggct
    26461 acagcgccgt cgtgttcatg ctgctgcgcg cgaccctcta cgtctatcaa ccgtacctgc
    26521 acgcacgcgg gctcggcccg atcgcgatcg gcgttctgtt cgctggcgta tacgtcgtcg
    26581 cgagcgcggt cgcataccgc acacacttgc tgcgccggcg gttcggcgac gagacgctga
    26641 tctgggcgct cctcgccgca ctctcgatca gcttcatcgg cctgtccggc gttgcgcagg
    26701 gaccgtggat gctgtcgctg ctcctggtgc aggccgtcgc aaacggcatg tactcgccgc
    26761 tgacgaagcc gctgctcaac gccgagatca cagactcgtc gcgccgcgcc gctgtcctct
    26821 ccgtcgagag catggcgcgc cggttcgcga tgggcctctt cgcgccgctc gtcggtctct
    26881 acggcgagac ggatgtgatg atgctgtgtg gcgtcgtcgg gctcgtcggc ttcgcgctgc
    26941 tcggcgttgc gcgcatgcgc aggtcggtcg cggatactta ctccgcgtag atcatcttca
    27001 cggtcatgcc gccgtcgatc gtgaacgtct gacccgtcac gaacgcagcc tcgctcgacg
    27061 cgagataggc cgcgagcgca gcgacatcct cgggacgacc gacacgcccg acgggatgct
    27121 gctcgtgatc caccttgcgc agctcgggag gcttgcgctc gttgcgcggc ttccacgcgt
    27181 cggtggcgat ccagcccggc gcgatcgcgt tgacgcgcac gtcgggcccg aggctcattg
    27241 cgagggcgtg cgtgagcgag atgatggcgc ctttcgtcgc ggcgtacgcc tcgttgttcg
    27301 gctcggccat gaatgcgcgc gttgacgtca ggttgacgat cgcgccacgg cgttcgcgga
    27361 gctgtgggat cgcggcgcgc gacacgacga aggtgcctgt caggttcgtc tcgatcacct
    27421 cgcgccactt gtcgaggccg agcgcttcgg gcgggccgtt atctggatct gcgatcgcgg
    27481 cgttgttcac cacgatgtcg aggccaccac cgaacgcgac agctgcatcc accgcgtgct
    27541 gcgctgccgc ttcatccgcg atgctgccgg tcaccgcacg cgtgcgctcg ccgagctcgt
    27601 ctgcagcatc ctcgaccgcg cgcttgttgt tgtcgaacag gacgaccttt gcgccttcat
    27661 cgacaaatcg cgctgcgatc gcgcggccga tgccttggcc ggcgccggtg atgagggcgg
    27721 tcagcccgtc gagtcgatgt ggcgtcatac ctaggtctag cgctgcggaa tggctcgcga
    27781 aagttcccaa ttgacttgct ccggacccca gatcagactg ccccgctcct gatggccgct
    27841 tccgcgcaac gcatctactt cgtctcgctt ggttgtccga aaaaccaggt cgacaccgaa
    27901 ttgatgctcg gccaagtcga agccgccggc cacgcgctcg ttcacgcacc cgatgaagcc
    27961 gacgttatcg tcgtcaacac gtgtgcgttc atcgacgccg cgaaagagga gagcgtcgac
    28021 accatcctcg agatggcgga gctcaagaag ggcgccgcac gcaagctggt cgtcaccgga
    28081 tgcctcgcgc agcgctacgc cgacgacctc gcgaaggaca tccccgagat cgatcacatc
    28141 ctgggctcgt ctgacttcca gtcgatttcg aaagtgctcg gcgcgcagcc gtcgaaggcg
    28201 accaaaggcc gcaagaagct gccgatggtg caggtaaccg agacgcccga atacatctac
    28261 gaccacgaag ctccgcgcgt tcgcatcggc gcgaaccact ccgcgtacgt gaagatcgca
    28321 gaaggctgcg accgcccgtg ctcgttctgc attatcccga agctccgcgg accgcagcgc
    28381 agccgccgca tcgacgacat cgtcgccgag gtcgcgcagc tcggccgcga gggcgtgcaa
    28441 gagatcaatc tgatcgcgca ggacctgacg cgctacggat gggatgcggg tagctcaccg
    28501 gagtcgcgcc agacgctcgc gcaactgctg cgtgcgctcg gcggcgtcga cggcatccag
    28561 tggcttcgcc tccactacac attcccgagc gcgttcgata acgacctcat cgatgcgatc
    28621 gcgagcgagc cgaaggtcgt caagtacgtc gacgttccgc tgcagcacat cagcgatccg
    28681 atgctaaagc gcatgcggcg cggtcactcg tcgcgcgtca cgcgtgagct cgtgaagacg
    28741 ctgcgctcgc gcatcgacaa cgtggtgctg cgcacgacgt tcatcgtcgg tcaccccggc
    28801 gagaccgatg ccgagttcga tgagctgtgc gagttcgtcg ccgaatcgaa gttcgatcgc
    28861 gcgggcgcgt tcacgtactc gatcgagccc ggcacgacga gcgcgatgct gccgaaccgt
    28921 gtggatcccg cggtcgccgc cgagcgccag cagaagctga tggagatcca gcgccagatt
    28981 tcgcgagcgc gtcacgaggc gatggtcggc aagacgatcg acgtcctcgt cgaaggcgaa
    29041 tcggacgagt cggagcactt gatggaaggc cgctggtacg gccaggcgcc tggtatcgac
    29101 ggcgtcacgt acctggccga cggtcgcgtc gcgccgggct cgatcgtcaa agcccgcgtc
    29161 acgcaagcat ctgattacga tctggccgcg tcgctcgagg tgtgacacca cctgctcggt
    29221 gcgccttggg ctgaaactgc ggccggttgc gcgtgtcttc ctctcgatga agtggctcct
    29281 cgccttggtc gctctctgcg gcgtcgcgca cgccggcgat gacagctgcg tcgatgatgg
    29341 caagccgtac gatcagaagg cgctcaaagc tcgcatcgag tacctcgcat ccgcggacct
    29401 cggcggccgc gtgccgggca ccgaaggcga caagctcgcg cgagcgcaca tcgtcgcgcg
    29461 catgaagtgc ctcggcctca ccgtgagcga gcaggcgttc aaggacaacg agaagaaaga
    29521 caccgcgaac gtcatcggca tcctgaaggg cagcgacgac agcggcgaga tcattatcgt
    29581 cggcgcgcat cacgatcacg aaggcaacgg ccacctaggc gcgaacgaca acgcatcggg
    29641 cgtgacaggc ctactcgcga tcgcgcaagc gatggcgcag aaggacgcga agccaaaacg
    29701 cacgatcgcg ttcatcgcgt tcggcgcgga agaggagggc gacctcggct cgcagttcta
    29761 cgcggcgcac gtccccgacg agctgccgat ggacaaggtc gtccagctca tcaacctcga
    29821 catgatcggc agctacaagt cgaagagcgt cgtcgcggcg atgggcacgt tcgcgaagtt
    29881 ccccgcgcgc aaagcgatcg acaagctcgt gaagggctac ccaaaaacac gcttcgcgct
    29941 cggcggtcac ggctatcggt cggatcacga ggccttctgc caacagggca ttccatacgt
    30001 cttcttctgg acgccggatg cgcgctgcta ccaccagaag tgcgacaagc cgtcggcgat
    30061 cgatctgccg cacatggcgg acatcgcatc gctcgccggt gacctgacgt gggagcttgc
    30121 aaactccgag acggacttgc tcgcttcgcg cacgaagctc ggctgcggcc agaagtaact
    30181 agcgcgtctt cgcgaacggt cgcgacgtca gaccgatcga caggccgaag ctgacgtcat
    30241 cgcgctcgag cacgcccacg ccggcttgcg ccgcgaggaa gacgtgcggc gacacccaca
    30301 acaggccgcg caggcgcgct tcgacgagcc cggacacgcc gttcgccgtc gcctgctgat
    30361 cgaattcttc ctcggacgcg ccgtacccgt ccgcctcgac ggtcgccgac accacacgca
    30421 cgcccgcggc cacctcgcca tcgagctcga gccgaccgct gcgcgtgcgc gcaccgatga
    30481 ccgcgtcggt gtcgatgagc gccttcgtcg tcatgtggag ctccggtgaa ttggtcatga
    30541 gctgtaccgg cgtgcgcgtc aggccaccaa tgccgagctc cgcgcctgca tacaggtgct
    30601 ccgagacggg catcgtgaag cgcatgttga acgtgccgcc ggccgtgtcg cccttgctcg
    30661 cgtcgccgct tccgatcgca tagctcgcca tgtcgccgtc gtaggtctcg agctgaccgc
    30721 tgtgcttcag ggctggcccg cgaaagcgat gcgcgaagcc gccgagctcg agcgaccacg
    30781 tcggcgccgt caggttcgca aacggattgc tgcccggcga gctgtcctct acgtacacgt
    30841 cgctgcccga cgagtagtag acgtcgccgc cactgtcgtg cgtgcgatga tcgcggacgt
    30901 ccggtgcatc gcccgagctg ccgccgtgcg tgcggtgatc gcgaacgacc ggcccgtcgt
    30961 cgtgcgtgcg atggtcgcga acgatcggcg catcgtgcga gtcgctgtcg tgtgtgcggt
    31021 gatcgcgaac gaccggcgcg tcgtttgaat cactcgagct gctgctcgtc cgatggtcgc
    31081 gaacgtcacc gccatcgtcg tgatgacggc cacctgccga cgcaatcgcg ggtacgaggg
    31141 caacgcttcc aacaagagcg atcattccag ctacgcgcat gggccccacc atgtgcagcg
    31201 cacagaccag tttcgggcac ttcgcacaac ttcatgttcg cgcgccgcca caaggatcca
    31261 gcggctctac gcgactggtc gtaccgccga tccagcaact cggtcacaca ctgttgccgc
    31321 agcaccacgg cctggtgcgt cagcggcacg cgtccaaatc caggccgaca caagttccgg
    31381 ccagtgcctg tcattgccag cgtggcatct gcgattcgag cttccgcgtc gtatcaggca
    31441 tacgacgccg ccttcgtgtc gttggatcgc tgacttgcac gctggtacct cacttgctgt
    31501 cgagcacccc atgatcgtcg gcgtcctcct cgtgttcgcc gtctatctga tcgttgcgca
    31561 ctggacgacg ctgctccagg gcgggtcggt cgtgccgttt gtgatgggct tcatcgcatt
    31621 tctagccgcc tggccggctc ttggctggaa gctcgcgttg cttgcgttct tcgtggatcc
    31681 aggcagcttg atgtgcgctc cgtggctcgt tgcttcgctg tgggaggagc gccgagccgc
    31741 gcgtcgctga gccgcggcgg gttacagagc tagcgtcgct tctttgtcgc cgctttcttc
    31801 ggcggtgctt tcttcggcgg tgctttcttc ggcggcgctt tcttcggcgg cgctttcttc
    31861 ggcggggctt tcgctggcgc ggctttcgct ggcggggctt tcgcgagcgc ggctttcttc
    31921 ggcgcggctt tcgctggcgg ggctttcgct ggcgcggcct tcttggcggg cgcagcctgc
    31981 ggcgatgcgc cctcgacgcg cggtagcggc ggcgcgccga ccgtcgggaa gtcgcgatgt
    32041 tcgccattcg cgccgaagca cgccgcgtac gggaacgctt gcttcgccgc ggcacacacc
    32101 gacgcgaact gctcgtgcca cttcacttga tcgatcgcga ggagcgcagc gacctcgtcg
    32161 tcgccaaacg agtagtagcc gaccgctttc tgctttgcgt cgcagagcgc gccgaacagc
    32221 cccggccacg cgggatgcgc gatcgccgcc ggttgcgcga gcacgcccag cagccagtcg
    32281 aacgtgtaga gatgctcgtc ggcgtagtag tcgatcgcgc cgccttcgtc gtcacccatc
    32341 ttcgagacgg gcagggcctt gccgtatgtc ttcttcgtca cgtcggcgta cgtcttcgac
    32401 agcgcgagca catcgagcac gagctgaggc cacgccgcat gcgcgatcgc cgcgggcgtc
    32461 gacagcactt gcttgcagac cgcttcgatc gcggcgcggc ccgcttcctt ctcgatcgga
    32521 ccaagcgcgg ctttcacgtc ggcgggccat gtcttgccgt ccaccttcgc gagcttcgcg
    32581 tacgacgcag ctgccttcgc ggcgtccttg ttcgtgccgc cgaccttctt ctcgagcgtc
    32641 gcgatgcctt cctcgacgcg gaacgctccg tctcctgccg tgggcagcat caggcgcagt
    32701 agctcgagca atttcggcgc gatcttcttg tcgacgcgac cccgcgcgat ctcgtcgagc
    32761 cacttgtcga cggcagattc ggtatagctc gccgtcgcga gcggcgatgc gcccgcagcc
    32821 agcagcgcct ttgcaatcgc gatggcgttc ggtcgccagc ccggctgcac gacgcagagt
    32881 gcgctgccga ggcagccgac gtggttcacg ttcgcgccgc gcgcgatgag cagctcgacg
    32941 atcttcacgt gatcggcgct cgccgccatg atgagcgcgg tctcgttgcg gtagtcgacg
    33001 ccgtcgaccg gtgcgccacc gtcgaggagc acctgcgtgc actcgaggtg ccccatgcgc
    33061 gccgcctcgc cgagcgggaa gtgatacgcg ccgccggtga tgcagccgtc ggtctgatcg
    33121 tcgtacgtct cgcagccatc gccgtcgagt ggcgcgccga tcgacagctg gtactggatg
    33181 ccctggagat tgccgtcgag cgccgactgg cagatggccg tgcggtcacg cataccgcga
    33241 ggttatcgcg agtcgcgcgc gtcaggattc gaggcggcga attcgcgcga gaccaatcgc
    33301 gagccaaata cccgccagga tcacgagcgt gatcgtcgcg cgcgtcgcgc tgccgtcggt
    33361 attgagcccc gacatgagcc agacctgctt cgtcacgctg atctcgcgaa tcgatgccgg
    33421 gatcgagccc atcgtcgggt cgacgacgac gagatagatg atcgccagcg ccatgccctg
    33481 cttgggcacg agcgtcgcga tgcctgccgc gatcaacgag accgcgacgg agcccagcgc
    33541 aaaggccgcg agctcctgcg ggttgatcgg cttgccggtg atgacggttg cgatcgccca
    33601 gccggctgcg gagagaaccg cggcgacggg cgcgagcgtc gcgattttgc cgaggagcag
    33661 cgaccagcgc gcgatgggcc gcgaccacat atacgtgatc gtgcggtctt cgatctcctc
    33721 gccgatactc gaggcgacga agatcggcgg caggatcgcc atcacgcgaa attgcgcgaa
    33781 ccgcatgagc tcggccatgt cccgatcgcc cctgaggccg aacgcgacga gggcagggag
    33841 cgctgcaatc agcgcgcaca cccacagcgc acggccgcgc agcaggcgct tccacgtgac
    33901 accgaacagc gtcatcgtga cggccatcaa gctggggatg gagcgatcgt tcgcgctcat
    33961 cgccggcctc cttcacccgt gaggtactcg aacaccgcgc cgaggttgtt gtcgggcgac
    34021 gtcatcgtcg tcacggcgat cgagctcgcg agcacggcct cgccgatgcg ctcgtagcag
    34081 cgatccggat cgcgcgtctc gatcagcacg gcgccacgct cgaagccgat ccgcgcgacg
    34141 tgatcctcgg gcgcgatgag cgacgccagc tgcctcggct tgtcacactc gacgcggatg
    34201 cgatgcgggt ggcgatcgat cagcttgcgg atctcgtaga tgttgccttc ggcgagcacc
    34261 tggccgcgat agatgacgac gatgtcggtc gtcagcgcct cgatctcgta gagcacgtgg
    34321 ctcgagatca cgatcgtctt gccggcctcg ccgagcttct tgatctgctc gacgatgtcg
    34381 atgcgcgctt tgggatcgac gcccgtcagc ggctcgtcga gcaagatgaa gtccgggtcg
    34441 tgcaccgtcg ccgtcgcgag cttcgtgcgc tggcgcatgc ccttcgagaa gcccttcacg
    34501 cggcgcgtct cggcgtacga catgcccatc tgcgcgagcg atgcacgggc ggcttcgcgc
    34561 gagcggttat gcggcacacc ggagagctcc gccatcgcgg tcacgagctc gagcgcggtg
    34621 agctcgtccc acgtcttctc gtgctcgggc gcgtagccga tccggcggcg cacttcgatg
    34681 tcggcgaacg ggctcttgtc aaagacggtg agcgtgccgc gcgatggctg cagcaggccc
    34741 gccatcatct tgatcatcgt cgacttgccc gcgccattgg gcccgaggag cccgacgatg
    34801 ccgcccttga gcgtccagct cacgtccgag acgccgagca cgtggccgta ccacttgctg
    34861 acgttgtttg ccgctacgac gctcacgacg agcctccgac gccggtcttc tgatcgcgtg
    34921 agacctggaa ccagatgatg cccatggcga cgatcacctg caggccgatc acgatcagcg
    34981 tgccgacgag cggcatcatc tcgaactcgg gtccgcgacc cgcgacgatc ttcaggtcga
    35041 acagctcgag cgtgaccgac tgcaggccgt attgcagatc gagcgcgccg atcggcgtgt
    35101 gcgtcgtcat cccgatgacg gtgaagatgc cgccgatgat cacgtagtac gcagcccaca
    35161 gaccgagcgc catgcggcgg ttgggcaaga tggccgacat cgcgagcggc atcgccgtgt
    35221 agagcagcgt cgcgagcgtg ccgagcgcga ggatcttcgg aatcatcgat gcgcgctcga
    35281 tgagctcgtc ggtggtctcg cacaagccga ggcgcaagcc gacgaggacg agcgggccga
    35341 cgagcacgat tgtcgcgacg aggaagccgt atccggcgag cttgccgagc atgtagtcgc
    35401 gcggcttgat cgatcgcacg aagtagaacg tgaacgcgcc gctctgcgtg tcgctcgcaa
    35461 tgatcgtcga gccgagcgtc aggctcagca cgaacgcggc ggtgcagaag aacttgaacg
    35521 agagcgggat gaccgcgtcg gccatcgtca ccatgatgtc gttcgggcca ccgatcgact
    35581 tcacgatctt gttggtcgag aagtacatca ggccgcccgc gatgcagacg gtgatgaacg
    35641 ccatgccgag cgcgagcttg tagcgccacc acttcttcca gccggtcgcg atctggtggc
    35701 gcatgatcac gagccagcgc gtcgatgcgg accggcgcgt gccgatgtag cgcttgtagc
    35761 cgagatcgtg gatgacactc atcgcgcggc tcctggagga gtgatgctgc cgggcgccgg
    35821 cggcggcgga acgatgggcg cttgctcgcc gatcacgcgc aagaacgcgg tctcgacact
    35881 ctcgcgacgg ccttcgacgc cgcgcacttg aatgccggct tctttcgcgc ggcggaacac
    35941 gagctcgctc gtcgcattcg gcggcagatt cacgagcacc tgcacaggtg acgtgaccat
    36001 gcacgacgca cccaccgcct gcattgcatc ggcgaacttc tgcgcgtcgg ccttcacctc
    36061 gaccatcagc tcgctgtcgc gaccgcgcgc gccgcgcaac tcgtcgatgg tgccgacaaa
    36121 tcgcacgcgc ccctgatgca tgatcaccgc gcgatcgcac acgcgctcga catccgggag
    36181 gagatgcgtc gagagcacga tcgcgcagcc gcgccgctcc ggcagctctg tgatgagcgc
    36241 cagcatctct tcacgcgagc gcgggtcgag gccgttcgtc ggctcgtcga gaaacaagat
    36301 ctctggatcg tggacgagcg cctgcgcgag ctggacgcgc tgcttcatgc ccgtcgaata
    36361 tccgtcgatc ggctggtacc gcttctcttc gaggcccgcg tagtagagcg cggcatgcgc
    36421 gcgctgcatc gcttcggtgc gcggcaagcc cgacagctcc gcggcatacg tgcacagctc
    36481 gaccgcgctc atgccggaca agaacgcttc ttgctcgggc atgtagccga cgcggtcgcg
    36541 aatctcgcgg ccttcggtct gcgtcgagag cccgagcacg ctcgccttgc cctcgtgcgg
    36601 gatgaggccg agcaagctct tcagcagcgt cgacttgccg gcgccgttcg ggccgaggag
    36661 cccgatgatg cgaccgtcga tgcgcgcggt gacgctgttg agcgccgtga tcgcgccata
    36721 gcgctttgtc acgccgacga gcgcgatcag cggcggcttg ttgctgcgtg cagcgtcctc
    36781 gtcggcgcgc acgctcgtgc ctgcgctgcc ttcgctaacg acctgcgcct tcggaagatc
    36841 cgtgctcacg cgaccacctt gggccacgcg cataccacga ccgaccgcac cgcgcccatc
    36901 gaatcgttcg tcaacgaagc caccctgcgc attcgaattc gcgtcgtcaa gcggcgcgat
    36961 ctgcagcgaa tcggccgcta tcgaatgact tcgatgcgca cgggcacgac accggcctcg
    37021 atcatgccga gcacgcgcgc ggacgcctcc gacatatcaa tgatgcggct cttgttccca
    37081 taaggcccac gatcattgat gcgcaccacg acgctgcgcc cgttgcgctt gttcgtcacg
    37141 cgcacgcgcg tctgcaacgg caatgttcga tgcgctgccg tcatcgcatt cttgttgaag
    37201 cgctcgcccg acgccgttgg cccaccgtgc aggctgccgc cgtaccaggt cgcgtagcct
    37261 tcctgcacgc cggtcggctt gccctgcgtc gtcttcttct gcccgccgcc acatgccgcg
    37321 agcacgacga gcagcagaag cacgacccac ttcatgcgtt gatgttagcg gtccggctat
    37381 tgcgtgtcga cgcagtccgc accgagctcg cgacccgctt tgtcgagcgc ggctttgcgc
    37441 agctggccgc ccgtcggtcc gtcgccgagc gtggcgcgtt ccttctcgcc gtggcccttg
    37501 ccgaggatgt cgcgcatgta cggcgtgaag tcgaacgtgt ccgagtgctc ctttgtgtgg
    37561 cactgcgtcg cgcacagatc ttttgccggc gcgcgcacga ccgcgaaagg cttctccatg
    37621 ccgcccttgt cgacgtgaat cgagccgggg ccgtggcact gctcgcagcc gacatcgcgc
    37681 aggttttcgt tgtgcgagag cgtcgagccg ccgggctgct cccagccggt cacgtggcag
    37741 ttgatgcatt cgtagtcgaa ctgctgcccg cgcttcacga gcgtctccca cgccttcgcg
    37801 tgcacggtcg tcttccagaa atcgacctgc tcttgatggc agtcgctgca cgcttcgttg
    37861 ccgacgtagc ccgctttgcc tttcgcggga ggcaccggca ctttgttcgc ggcagccttc
    37921 acgttcgctt cgccggccgc ggcgttgtag cccttgatcg cgtcgcgcac gggcgggctg
    37981 cacgcgagct gcttgttgat gcgcagctgc tcgagcgtga agtagctgcc cttcgccggg
    38041 acgacgagtg gctgcgcttc gagttgcttc ttctgcgcgg cgacctcggt gcgctccgtc
    38101 tctttcttcg cgatgaactg cggatcgctc gacggatcgg ctttgaattt cgcgatcgcg
    38161 gcgtcgagct tgccgagctc gtgatcgagc tgcgcgatct tcactttcgc ggcgccgggg
    38221 ccgactgcat cgacgagcgt gccgccgccc gcgcgcagcg tgacgtcgac gcggctgagc
    38281 acttggccgc gattgccggg aatcacgagc cagccgtcac cgatcttctc cgcttcgacc
    38341 gagacgtcct ccggctctgg cgcgaccgcg ccgaggcccg cgatcgatag atcgatagcg
    38401 ccgatatctc gaatcagggc gactgcatcg cgcttgctgc tcgcctgcac gagcgcgacg
    38461 acgacctgcg cgccgccttt cctcaattga tcgaccgcgc gctttccggc ggcgaccggg
    38521 tcatcgacct tcacatcctt cagcgtgtct cgagcgatca cgccgaacac gccgaccttc
    38581 gcatcgccga cggtgaacac ctcgggcgcc tgcagcttga acgcggggtc gctgacgttg
    38641 gccgcttcac gcggaaaccg cacgctcccc gcgcccttcg ccaagtccgc cgggccgagg
    38701 cccaccgcgg cgaccttgag gttctcctta tagatagagg ccaggaggtt cgccttgaga
    38761 tcctcttgcg cctcgaggtg cgcgggcagg ggattcttcg catagaggag cgatccggcg
    38821 tccacgacga ggaccggacc cttcgcgcgg gcctcctcca cgagccgcgt cgtccgcgaa
    38881 atgtctccga gaggatcgga ggtacagcca cacggaccga tctggccgcg cacttcggcg
    38941 agggcaaaga ccgtaaagct gggcttcgca ggcgcgcttg gagtgcccga tccgccgcct
    39001 ttcacctctg atttcgagga acaagccgcg accacgacga gccccaggac agctagctgc
    39061 cggcgcatag gccgctcata tagcacgttg acagctcgta agtgcctggg tagattgccg
    39121 cgctctcgca cagaggatcc catggctgtc gctattcgca tgtcccgtca gggcaagaag
    39181 aagtctccgt tctatcgcat cgtcgccgct gacaagcgcg ccgctcgcga tggtcgattc
    39241 atcgagctgc tcggcacgta tcacccgatc accaaggcac tgaagctcga catggagcgc
    39301 tacttgaagt ggctctccgt cggcgcgcag ccgagcggaa cgctcgctgc tgtcattcgt
    39361 cgtacggcgc gcgctgccaa ggccgcgtaa tcgacatggc gaaagagcct gatctggccg
    39421 agctcatccg ctatctcgcg gtgaatctcg tcgacaagcc cgacgaagtt cgcgtcgagc
    39481 tggtcgaaga acgccaggca aatgtctacg agctcgaggt cgctgagtcg gacctcggca
    39541 aggtcatcgg ccgcggcggc aagacggctc gcgcactgcg caccgtcgtg caggcggtcg
    39601 caccgcggtc tcgcaagcgt acgctcgtcg agatcctcga ataaaccggc cggtcggcga
    39661 gcgctcccat gcgtggtgat gcgcgtatcg aaatcggcgg gattgcccgc gcgcacggca
    39721 tcaagggcga ggtcgtcgtc gtcacgcacg accccgactc cgagacgctg ggcaagatca
    39781 cctcgatctg gatcgagggc accgaataca agatcacggg cgcgcgcgac acccagcgcg
    39841 gttggctcgt cgcgctcgaa ggcatcgtca cgcgcaacga cgccgagcgc ctgcaaggca
    39901 agcagatcga agtcgagcgc tcctcgctcg agctcgacga agaagacgtc ctgctcgacg
    39961 acatggtcgg ctgcgaagtc cgccttccgg acggcacgcc ctggggcacg gtagtcgcca
    40021 tcgacatctc gaacccgcac caagatctgc tcgtcgttca cgacggcgat accgagcgcc
    40081 ttctcccgat catcgatcag ttcgtgacga acgtcgatct cgatgcgggc atcgtcatgg
    40141 tcgatccgcc ggaaggcctt ccgtcatcga aggtaccgaa gtgacgccac gcgagctcgc
    40201 acgatgaagt tcaaggtcgt caccatcctg ccggagctga tcggccctgc gctgcgtgcc
    40261 ggcgtcgtcg gtcgcgcgtg cgaagccggc atcatccagg tctacacggt caatccgcgc
    40321 gacttcacgc gcgatcggca caagacggtc gacgacacgc cgtacggcgg cggcccgggc
    40381 atggtgatga aagccgagcc gctgctcgca gcgatcgagt acgcgtcgga gccgccggat
    40441 cccgattccg tcgaagccac gcccgaggcc gacatgatcg ccgcgccggt gtcgagggtc
    40501 gacgcggact tgccgggcga tccagacgac gtgatgtcga caggccgctt cgatcgcgcc
    40561 gctctgcgcg acctcgccga tgccgacgac ggcgacatca tgacgacggg ccgcttcacg
    40621 cgcccctcga ccgccgactt gcaagggcgc gcgccagcgc agggcgacgc aacgtcacgc
    40681 gcggacgcgc tcgctcgcgc gaaagccgcc gaagaggcgc gcaagaaggc cgccgacgat
    40741 gccgcgaagc gcgcgctcgc cgccctggaa ggcacatcga cggacgagca aggcgacatc
    40801 ctcgacgacg aagccgcaca acgcgcgctc gacgagctag agcgccaagc gcgcgaccgc
    40861 gaagcgggcc tccaccacgc gacgacaaag cgcgaagaag tgcgcggcac gctcagcgcc
    40921 gtcgattcat cgatcgacaa gctcgaaggc gccctcggcg gcaacctcga cgcgctcgcg
    40981 cctctcggcg ctccgcgagc ttcgacaggc tcgtcgcgtt cgacaggctc gtcgggttcg
    41041 acaagctcgt cgggttcgac aagctcgccg ggttcgacaa gctcatcgcc ttcgacaagc
    41101 gcttcggggg agtcggcgcc gaagccgcat cggattcttc tttcgccgtc gggcaagccg
    41161 ctcacgcagc agcgcgtacg cgagctcgcg aagctcgaac atctcgttct cgtgtgtggt
    41221 cggtacgagg ggatcgatca gcgcgtgatc gatttggcca tcgacgagga gctctcgatc
    41281 ggcgactacg tgctgtccgg tggtgagctc ggcgcgctcg tcatcatcga tgcagtggcg
    41341 cgttacgtgc cgggcgtcct cggcgaagcg acgtccgtcg acgacgaatc gttctcggct
    41401 ggactgctcg agtatccgca gtacacgaag cccgcggagc tgcgcggaca ggccgtgccc
    41461 cctgtgctgc tatccggcaa tcatgcgcac gtggcggcgt ggcgtcgcga gcaggcgatg
    41521 cggcgtacgg cggcgcggcg tcccgatctc ttgcgcgcgt ttgtgccgac gaaggccgac
    41581 gcgaaagccc ttccgccgct gcgcgcgcgc acgcatctcg cgctcgttca tcatccagtc
    41641 gtcgatcgca cgggcgcggt catcacgacc gccgtcacga acttcgacat tcacgacctc
    41701 gcgcgctcct cgatgacgtt tggcctcgcg ggctatcacc tcatcacgcc cgtcacctcg
    41761 cagcgcgaga aggcgcagca catcgcggag ctctggatcg gagacgagca gggcgagcat
    41821 cgcgcgcgcg cccttcgcct tgtccgggcc gcggactcca tcgagaccgt cattgccgcg
    41881 ctcactgcgg agcacggccg gccgccgctc gttgtcgcga ccagcgccaa gccgacgtca
    41941 tttccgggag cggcccgccg aacgtcgtcg gagctgctcg cagaagcgac ccttgacccc
    42001 tcgccgatgc tgattcttct cggtacgggg tgggggctgg ccgatcacct gattccgtcg
    42061 gtatcccgca tccttgcgcc gatcgaagga gcttcggact ggaaccattt gtccgttcgc
    42121 tccgcgggag ccgtgattct cgaccgtctg ttcggtcgcc ctgcttgacg gcccggggtg
    42181 ttctcccgta gaaccctgcg tccaatcggt accttgcgaa ggctttagca atgagcgctc
    42241 agcggattct cgactccatc gataagagcc aacgtcgcac ggcggcactc cccgagttcc
    42301 gccccggtga ctcgatcaaa gtgtgggcaa agattcgcga aggcgagaag acccgcctcc
    42361 aggcattcga aggcgtgtgc atccgccgcg tgtccaaggg cgcgcggtcg acgttcacgg
    42421 tccgcaagat cagctacggc gtcggcgtcg agcgtatctt cccggacaac tcgccgaaca
    42481 tcgacaagat cgaagtgatc gcgcgtggtc gcgttcacca gtcgcgcctc tactatctgc
    42541 gcgacctctc gggcaaggcc gctcgcatca aggagcgcga gggcacgaac gaagaagccg
    42601 ccaccgcgac ggccagcccc gcagccgacg gctcggagac gcccggcgcc gaaggcgctg
    42661 gcgacagtgc caggaccaag cgcaagaagg acaaggcgaa gaagtcctga ttcgctcgcg
    42721 gcgcttcacc gcgctgccga cgaccaacga tcgaggcccg cacgtcgggc ctttttccgt
    42781 ttcggtgacc tgcgcgccga ggcgctttcg ataagctctg acacgcgatg gcgaagacac
    42841 cagcgaagcc acagcagtcc gatccgctct tcgcggcgct caaggcgctc gtcaaaccgt
    42901 acgcaaaaca cttcaaggtc ttcgaggata ccgaccagca ctacgacctc gaagaagacc
    42961 tccgctacag ccgcacgatg ttcgcggtcc tctacaagcg ccctcgcgaa gtccgcttct
    43021 acttctaccc gctcaacgtc tggcccgatc tgcgcgagct gctgccggca tctcttcagt
    43081 cacaaatcac aggcaagtgg atcatcaagg tcgccgtcct ggggccagcc gagaagaagg
    43141 cgctcgccaa gatgctcgcc gaggcgtggc gccgcatcgc cgcgatccgc gcgctcgcga
    43201 agcagcatcc cttctacaga aagttcgatc tcgaggacac gcacgcgctc atcacgaaga
    43261 tcgcgaggaa agcgaccgtc gagcttcgca agaaggacgt gcaggtcacg ctccccaaca
    43321 cgccgaccgt catcccagcg gtgctcggcc cgcgcgtctt caaaccgggc atcttccgct
    43381 tcaagtcgat cacgcccgat gagcacgccg cgctcgccgc actgacgcgg tagaagacac
    43441 gtccgccacg atcaacccgt tggtcgctgc tcgtgccttg gcgttgcaac ctagacgaag
    43501 ctcgcgctcg ggtgacggcg cgatcacttc gcgtgcacga cgcagtccca gctgacttcg
    43561 gtgactgcgc ccgctgtgac ctcgaccggc ttcgcgcgct cggtctcccc gcacgagctc
    43621 ggtatcgcgc acgcatcgaa cgaaaccgtg tagcggccgg cgggcacgcg catctcgaat
    43681 ccgcctgtct cggctgcgct ccgagcgtcg gtcgtcttgc cccacgcgtc tcgcgcgcta
    43741 acggtgcatt cgctaccggt cgtggtcgaa aacgcctcct ccggcgcgtg cgcgcgaccc
    43801 ggaccatcga gtgtgtgcgt gacgcgaccc ttgattgtgc ccacgccgtg ctgcgctggc
    43861 ggaacctcgc ttcccgcgcc gaccgccgcg gccgtctctc gcgtcgcacg ctcgctctcg
    43921 cgcgtcgcac gcgcgccttc gccttgatcg cacgcgacga agcagaagca agcgagcacc
    43981 gcgagccgaa gtcgaacgcc tacgcgcatt cggcaagcgt acgttgccgg cgctcgcgcc
    44041 acaacacccg ccgatgctac ccgcggcgac tcgagcccgg ccgatgtccg agaaccggac
    44101 acgctagctc ccgcgccggc gtcaaacacg cgcgttggcg tcagacaccg ggcgtatgac
    44161 gaaacgcatg aagtcgaacg aatcaactcc gtggtcacgc gccgtcgagc gcgcacgcgg
    44221 caacgccgac gatatgaagc cgcccgcatc gaacgcgcac ctcgagcgcg acaatccctc
    44281 gatgaggcaa ctggtgtcga aggcgcacgc agaccgcgca cccgacaatc cctcgatgag
    44341 gcaaccggtg tcgaaggcgc acgcagaccg cgcacgaggc aacgccgccg atatgaggcc
    44401 gcccgtgtcg aacgcgcacc tcgagcgcga caagccctcg gtcaagcggc ccgtgttgcc
    44461 ggcgcaacgg gactgcgagc aatcgtcggt gcagccgccc gtatcgaagg cgcagtgtga
    44521 ccgcgacaag ccctcggtca agccacccgt gtcgaaggcg cagtgtgacc gcgacaagcc
    44581 ctcggtcaag ccacccctgt cgaaggcgca gcgtgaccgc gacaagccct cgctcaagcc
    44641 acccgtgtcg aaggagcagc tcgagcgcga ccgccaggta ctcatgttcg acgacgcggc
    44701 gggaccaccg acgaccatcc accgcggcaa cgcgagcgag gcgcacgctg cggcatatct
    44761 cgagcgcgtc ggtatgcgca tcatcgagcg caacttcaag acgaggctcg gcgagctcga
    44821 catcatcgcg cgcgacggcg ccaccctcgt cttcatcgaa gtgcgctcgc gctcgacgac
    44881 gacattcggc agcgccgtcg aggccgtgca ccaccacaag caacgcaagg tgacgcgcat
    44941 cgcgtggcag tacatcaaag tgcgacggcc gcggttcgac acggcgcgct tcgatgtcgt
    45001 cggcatcacc gcaggtgagc tcgtccacat ccgcgacgcc tggcgcatcg cgtgacggtc
    45061 gaggaagccg tgtcgatttc cctccgcgcg ccaggcgggc cacttcgacg cgattcaagt
    45121 ccacactcca accgagtagg cgtcgtcgca gttcccggag ttttccccga cagctcagct
    45181 ggaccatcca ggccaccgac gtgagctgtc gggcaagcga gccgcgacca acgtacttgc
    45241 tacgagagcg ctgcgacctt gcggagcgcg acgtcgaccc actgttccag cgtcaggttc
    45301 tgactgccca cgtgggtacg agtcgcgtcg accgccgcct tgacgagccc cttcgcgacg
    45361 ccacgcttgc gaagctccgc cgcacattcg accgcgcgac gcgccatctc gaacttgctc
    45421 tgcgcccgaa acgtaaaccg tgcattcgtc gcatcgcctt cgatgatcaa cgtgcccgcg
    45481 tggagcgcga gatggtggcc ttcgcacagc gcgatcagat ttggcgcctc gttcgggccg
    45541 ccgttgcaca cgaatacgat gtggtggcag tcgacattcg cggtcgcgct gcatccaggc
    45601 acgcggcatc gatagccgtc gcgatgcagc acatgacgct tcagcgcctt cgaaatgctg
    45661 cgcttcgcgc ggccaggctc gtcatcgtcg atatcgccga tatcgatgga gtcgcagagc
    45721 gcgcgttcca tctccggcgg cgatagcggc gtcgtggtcg cgccgtgctg ccatccgcgc
    45781 ttgcagctat cgcactgagt gatcgcgatc tgatagcgcg gcgctaacag ccgctcgacc
    45841 tccgcgttgg tgagcgtgcg tccaccgatg atgatgtccg cggaggtgga gcgcgtgctc
    45901 ctacccgcgt ggctctcgat cacgcaggtg gccgccgagc tcatacccac gtgggtctcg
    45961 ttcgggcggg ttgacgccga cctcataccc acgtgggtct cgatctcgca ggtggccgcc
    46021 gagctcatac ccccgtgggt ttcgctcggg cagatggacg ccaagctcat acccgcgtgg
    46081 gtttcgttcg cgcaggtgga cg
//
LOCUS       DATLBZ010000637         5849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_11031987, whole genome shotgun sequence.
ACCESSION   DATLBZ010000637 DATLBZ010000000
VERSION     DATLBZ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11031987"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /gene="treS"
                     /locus_tag="VMZ53_31460"
     CDS             complement(<1..1296)
                     /gene="treS"
                     /locus_tag="VMZ53_31460"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HUS33076.1"
                     /translation="MSEELPDDPLWFKDAVIYEIHIRAFQDATNDGIGDIQGLISRLD
                     YLADLGVTALWLLPFYPSPLRDGGYDIADYTNVHPSYGTLEDVATLLREAHRRGMRVI
                     TELVLNHTSSEHPWFQRARRAPPGSPERNFYVWSETPAPYSDTRIIFQDYETSNWAWD
                     PVAKAYYWHRFYSHQPDLNFDNPAVHEALFGALDMWFEMGVDGMRLDAVPYLYEREGT
                     SCENLPETHDFLKKLRRHIDGKFKNRMLLAEANQWPADAAAYFGDGDECHMNFHFPLM
                     PRMFMAIELEDSFPIINILNRTPRIPDSCQWATFLRNHDELTLEMVTDEDRDTMYRAY
                     AAERAARVNLGIRRRLGPLLGVRRKIELMNALLLSLPGTPVLYYGDEIGMGDNIYLGD
                     RDGVRTPMQWSGDRNAGFSRANPQKLYLPPIIDPEYHYEA"
     gene            complement(1293..3284)
                     /locus_tag="VMZ53_31465"
     CDS             complement(1293..3284)
                     /locus_tag="VMZ53_31465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HUS33077.1"
                     /translation="MHSAQVHGQAELAPSRVIILAIRPEVDGGRYAAKRVVGDRVEVE
                     VDLVTDGHDVVRGVLLHKPPGATAWEEIELVSAGNDTWCASFTATALGRHAYTAIAWV
                     DAFGSWRHGFERKVNAGNDVSVELVEGAMLVEAATKRSQDPALAKYAAALRGSQPIGE
                     RIALALGPELTDAMNRAPDRPHAARYRSDLELHVERPLAASSTWYEMFPRSMGPAGRH
                     GTLRDAEKMLDYVAELGFDIVYLPPIHPIGRAFRKGPDNSPTAGPDDPGSPWAIGGPE
                     GGHTSVHPQLGTIADFDHFVGEAAKRKLEVALDIAFQCSPDHPWVKQHPDWFKQRPDG
                     TIQYAENPPKKYQDVYPFDFESRDWKNLWAALRDVFLFWAGHGVRVFRVDNPHTKPIP
                     FWEWCLREVQAKYPDAIFLAEAFTRPKLMYALAKSGYSQGYTYFTWRVTKADLESYLR
                     ELVKPPTVDLYRPNFWPTTPDIFPDHLVHGGRPAFVQRVILAATLGASYGIYGPSYEL
                     LEHEPRPGVEELAQNEKYQLRTWDVNRPDSIRHVIARLNKIRREHPALSDMRSLQFHG
                     TDNELVMAYSKQRGDSTILCVVNLDPHHRHRAWLDLDLEALGVPHDRTFQVHDLLSGA
                     RFAWRGSRNFVEIDPAQIPAHIFELRRFARSENQFEYFL"
     gene            complement(3382..3609)
                     /locus_tag="VMZ53_31470"
     CDS             complement(3382..3609)
                     /locus_tag="VMZ53_31470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33078.1"
                     /translation="MTTKQNKPERPEEYERSNEGDEGASILQKEQSSFVDEDSPRGKQ
                     FGASRKVADQDRVGAASPDADSDELPKQPQK"
     gene            complement(3639..4130)
                     /gene="yjgA"
                     /locus_tag="VMZ53_31475"
     CDS             complement(3639..4130)
                     /gene="yjgA"
                     /locus_tag="VMZ53_31475"
                     /inference="COORDINATES: protein motif:HMM:NF003593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HUS33079.1"
                     /translation="MNSDDENPRVLAKRQKRAAGDRSAALARTLMQLPDTTIEKLELG
                     EDLEHEVTQARRITSQIARRRAERSLAGSLRGEDLLELQRKLENVQETGSSEPRLFHL
                     AEQWRARLIEEGIAAAAELPGGAADPLPKLIQNARRERDTGKPPGAARALFRHVMQLL
                     KKG"
     gene            complement(4146..4745)
                     /locus_tag="VMZ53_31480"
     CDS             complement(4146..4745)
                     /locus_tag="VMZ53_31480"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS33080.1"
                     /translation="MKAVRDALAAMTAYPTAILRAIIAGDAPRERHRAVPRDPEFMQR
                     ITLGLTTMSSDGRMIAVQVNGYAGMLTVVCAAWRQAKTLIMTTVEDRVDHVIAGIGAM
                     ARESLFLVYEGRRYPVKREPFVIGRSKVCDLRIRDGCVSRRHAAVMYKNGRYYIVDLE
                     SQAGIEYRGMKIRNKRIEEGDMFKLHEYALRFTFHETDA"
     gene            5349..>5849
                     /locus_tag="VMZ53_31485"
     CDS             5349..>5849
                     /locus_tag="VMZ53_31485"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HUS33081.1"
                     /translation="MLLFKKAFHAGLVDGSIRVTFRAWDKPHVRVGGRYRCHPIGVLE
                     VDAVTKVRLADITSADAALAGFTSRDELLAFIRSGPRGDAMTDDSELFRIELHHGGDG
                     DRVEIALDDQLTDEAIATLATKLARMDGKKPWTRETMQLIAKHPRLAASKLAAKVARE
                     TLPFKID"
BASE COUNT          997 a         1888 c         1874 g         1090 t
ORIGIN      
        1 cgcctcgtag tgatactcgg gatcgatgat cggcggcaga tagagtttct gcggattcgc
       61 gcgcgagaag ccggcgttgc gatcgccact ccactgcatg ggtgtgcgca cgccatcgcg
      121 gtcgccgaga tagatgttgt cgcccatgcc gatctcatcg ccgtagtaca ggaccggcgt
      181 gccgggcagc gacagcagca gtgcgttcat cagctcgatc ttgcggcgga cgccgagcaa
      241 cgggccgagc cgccggcgaa taccgaggtt cacgcgagca gcacgctcgg ccgcgtaggc
      301 gcgatacatc gtgtcgcgat cttcgtcggt aaccatctcg agcgtgagct cgtcgtggtt
      361 gcgcaggaac gtcgcccact gacatgaatc cgggatgcgc ggcgtgcgat tgaggatgtt
      421 gatgatcggg aagctgtcct cgagctcgat cgccatgaac atccgcggca tcagcgggaa
      481 gtggaagttc atgtggcact cgtcgccatc accgaagtac gcagccgcgt cggcaggcca
      541 ctgattggct tcggcgagga gcatgcgatt cttgaacttg ccgtcgatgt ggcggcgcag
      601 cttcttcaag aagtcgtgcg tctcgggcag gttctcgcag ctcgtgcctt ctcgttcgta
      661 gagatagggc actgcatcga ggcgcatgcc atcgacgccc atctcgaacc acatatcgag
      721 cgcgccgaac aatgcctcgt ggaccgccgg attgtcgaag ttgagatccg gttggtggga
      781 gtagaagcgg tgccagtagt acgccttcgc gacggggtcc cacgcccagt tcgacgtctc
      841 gtagtcctga aagatgatgc gcgtgtccga gtacggcgcg ggcgtctcgc tccacacgta
      901 gaagttgcgc tctgggcttc ccggcggtgc gcggcgcgcg cgttgaaacc acggatgctc
      961 gctcgacgtg tgattgagca cgagctccgt gatgacgcgc atgcctcggc ggtgcgcctc
     1021 gcgcagcagc gtcgcgacgt cctcgagcgt gccgtaactc ggatgaacgt tcgtgtagtc
     1081 ggcgatgtcg taaccaccgt cgcgcagcgg cgacgggtag aagggcagga gccagagcgc
     1141 ggtcacaccc aggtccgcga gatagtcgag ccgcgagatc aagccctgga tgtcaccgat
     1201 gccgtcgttc gtcgcgtctt ggaacgcacg gatgtggatc tcgtagatga ctgcatcttt
     1261 gaaccacagc ggatcgtcgg gaagctcttc actcataaga agtactcgaa ctgattctcg
     1321 ctcctcgcga accgtcggag ctcgaagata tgtgccggta tttgcgccgg atcgatctcg
     1381 acgaaattac gcgaacctcg ccacgcgaag cgcgctccgg acaagagatc atgcacctgg
     1441 aacgtccggt cgtgggggac gccgagggcc tcgaggtcga ggtccaacca cgcgcggtgg
     1501 cggtggtgcg gatcgagatt gacgacgcag agaatcgtgc tgtcgccgcg ctgcttcgag
     1561 tacgccatca cgagctcgtt gtccgttccg tggaactgca gcgagcgcat gtccgacagc
     1621 gctggatgct cgcgccggat cttgttcagg cgcgcgatga cgtggcgaat cgaatcaggc
     1681 cgattcacgt cccacgtgcg cagctgatat ttctcgttct gcgcgagctc ctcgacgccg
     1741 gggcgcggct cgtgctcgag cagctcgtag ctcgggccat agatgccgta gctcgcaccg
     1801 agcgtcgccg cgaggatgac gcgctggaca aacgcaggcc gcccgccgtg cacgagatgg
     1861 tcgggaaaaa tgtcgggcgt cgtcggccag aaattcggcc gatacagatc cacggtcggc
     1921 ggcttcacga gctcgcgcag atacgactcg agatccgctt tcgtcacgcg ccacgtgaag
     1981 tacgtgtagc cctgcgagta gccgctcttg gcgagcgcgt acatgagctt tggtcgcgtg
     2041 aacgcctcgg caaggaagat cgcgtcgggg tacttcgcct gcacttcgcg caggcaccac
     2101 tcccagaacg ggatgggctt cgtgtgcgga ttgtcgacgc ggaacacgcg cacgccgtgg
     2161 ccagcccaga acagaaacac gtcacgcagc gcggcccaca ggttcttcca atcgcggctc
     2221 tcgaaatcga atggatagac gtcctgatac ttcttcggcg gattctccgc gtattgaatc
     2281 gtgccgtccg gccgctgctt gaaccagtca gggtgctgct tcacccacgg atgatcgggc
     2341 gagcattgaa acgcgatatc gagtgcgacc tcgagcttgc gcttcgcggc ctcgccgacg
     2401 aaatgatcga agtccgcgat cgtgccgagc tgcggatgca cgctcgtgtg tccgccttcc
     2461 ggaccgccga tagcccacgg acttcccgga tcatcggggc ccgcggtcgg tgaattgtcg
     2521 gggcctttgc ggaacgcgcg tccgatcgga tgaatcggcg gcaggtagac gatgtcgaag
     2581 ccgagctcgg cgacgtagtc gagcatcttc tcggcgtcgc gcagcgtgcc gtgcctcccg
     2641 gccggtccca tcgagcgagg aaacatctcg taccaagtcg acgacgccgc gagtggccgc
     2701 tcgacgtgaa gctcgaggtc actgcgatag cgtgcagcat gcgggcgatc aggcgcgcga
     2761 ttcatcgcat cggtgagctc gggtccgagc gctagcgcga tccgctcccc gatcggctgc
     2821 gagccacgca acgccgctgc gtacttcgcg agcgccggat cctgcgagcg tttcgtcgct
     2881 gcttcgacga gcatggcccc ttcgaccagt tccacgctga cgtcgttgcc ggcgttgacc
     2941 ttgcgctcga agccgtgccg ccacgagccg aacgcatcga cccacgcgat cgcggtgtac
     3001 gcgtgccggc cgagcgccgt cgcggtgaag ctcgcgcacc acgtgtcgtt gcccgccgag
     3061 acgagctcga tctcttccca cgcagtcgcg ccgggaggct tgtgcagcaa cacaccgcgc
     3121 acgacgtcgt gaccgtccgt aacgagatcg acttcgacct ccacgcgatc gccaacgacg
     3181 cgcttcgccg cataccgccc gccgtcgacc tcgggccgaa tcgcgagaat gatgacgcgc
     3241 gagggtgcga gctccgcctg gccgtgtacc tgcgccgaat gcatgcccgc cggagaaaac
     3301 cctagcgccg gattcgagat cggtcgcttc gacatcaccg cctacctggc gcctaacctg
     3361 tgctcaggcg ctcgcccgga gtcacttctg cggttgcttc ggcagctcgt cggagtccgc
     3421 atcgggagac gcagcgccga cgcggtcctg gtcggctacc ttccggctcg cgccgaactg
     3481 ctttccgcga ggcgaatcct cgtcgacaaa cgacgattgc tccttctgca ggatcgatgc
     3541 gccctcgtcg ccctcgttgc tccgctcgta ctcttccgga cgctccggct tgttctgctt
     3601 cgtcgtcatg ccgctaaacg cagcaagccg cgagccacct agcccttctt gagcagctgc
     3661 atcacgtggc gaaacaacgc acgcgccgca ccaggaggct tcccggtatc gcgctcgcgt
     3721 cgcgcgtttt ggatcagctt cggtagtggg tcagcagcac cacctggcag ctcggcagcg
     3781 gctgcgattc cctcttcgat caagcgcgcg cgccactgct cggcgagatg aaacaggcga
     3841 ggctcgctgc tgccggtctc ctgcacgttc tcgagcttgc gctgcagctc gaggagatct
     3901 tcaccgcgca acgagcccgc gagcgatcgc tccgcacgcc gccgtgcgat ctgcgacgtg
     3961 atgcggcgtg cttgcgtgac ctcgtgctcg agatcctcgc cgagctcgag cttctcgatc
     4021 gtcgtgtcgg gcagctgcat gagcgtgcgt gcgagcgccg ctgacctgtc gccggccgcg
     4081 cgcttctggc gcttggcaag gacacgcgga ttctcgtcgt cgctgttcat cgtcgacacc
     4141 gtacctcaag cgtcggtctc gtggaacgtg aagcgcagcg cgtactcgtg cagcttgaac
     4201 atgtcgcctt cctcgatgcg cttgttgcgg atcttcatgc cgcgatattc gatcccggcc
     4261 tgcgactcga gatcgacgat gtagtaccgg ccgttcttgt acatgacggc cgcatgcctg
     4321 cgcgacacac agccatcccg aatccgcaga tcgcagacct tgctacgacc gatcacgaat
     4381 ggctcgcgct tgaccggata tcgacgtcct tcgtagacga gaaaaagtga ctcgcgagcc
     4441 attgcgccga taccagcgat gacgtggtcg acgcgatcct cgacggtagt catgatcaaa
     4501 gtcttggctt gccgccatgc cgcacacacg accgtcagca tcccggcgta gccgttcacc
     4561 tgcaccgcaa tcatccggcc atcggagctc atggtagtga gaccaagggt gatgcgctgc
     4621 atgaactctg ggtctcgcgg cacggcgcga tgccgttcgc gcggagcgtc cccagcgatg
     4681 atcgcgcgca gaatcgcggt cgggtaagcg gtcatcgcgg ccagtgcgtc gcgcaccgct
     4741 ttcacgacgg tctctggatc ggcggccttc gtgagtcgtt cgtacatgag cactctgcag
     4801 gcgaatccga ccgcgcgcgc cgtctcagtc acgtcgataa cctcatcaac ctgaatgggc
     4861 cgatgcgatc gctcgaccgc gctgctcgca agtccgccga agccacgcca ctcggcctcg
     4921 agctggctcg atgttgcgtt cacgtccggt ggttcgcgtg cggccgcgat gccgtctccc
     4981 gcgatacccg ggttggtgac ttcgagatct tcggcttcgc cagctggccc gtcactcgag
     5041 tggcgggcgg caagcacgtg ccggcgggcc gtcgtgtagc tctcgcctgt cttggcttgg
     5101 cggtcgcgga tgcgcttctt cagatcgcgc ttggctgtca tcggtccttc ctctcggcac
     5161 cgcgcggcga ccccatgcat caccccgcgt tgcgaggcgg aagaaaccag cttgttccgc
     5221 tcgagaagag gactcccctt tgccttcatc gtgcgatcgg ggcatgggcc gatcgtcgaa
     5281 ggtcgtgcgc gagcagcggc gcagcaccga gcttagcaat gtagagtgcc gccgagaagc
     5341 gggtctcgat gttgctgttc aagaaggcat ttcacgccgg gctcgtcgat gggtcgatcc
     5401 gcgtgacctt tcgcgcgtgg gacaagccgc acgtgcgcgt cggtggtcgg tatcgctgtc
     5461 atccgattgg cgtgctcgag gtcgatgcgg tcacgaaggt tcggctcgcc gacatcacgt
     5521 ccgccgacgc ggcgctcgct ggcttcacat cacgcgacga gctgctcgcg tttatccgca
     5581 gcgggccgcg cggtgacgcg atgaccgacg acagcgagct gttccgcatc gagctgcatc
     5641 atggcgggga cggcgatagg gtcgagatcg cgctcgacga ccagctcacc gacgaagcca
     5701 tcgcgacgct cgcgacgaag ctcgcgcgca tggacggcaa gaagccgtgg acgcgagaga
     5761 cgatgcagct catcgcgaag catccgcgcc tcgcggcgtc gaagctcgcc gcgaaagtcg
     5821 cgcgagagac gctgccgttc aagatcgac
//
LOCUS       DATLBZ010000638         2654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3600543, whole genome shotgun sequence.
ACCESSION   DATLBZ010000638 DATLBZ010000000
VERSION     DATLBZ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3600543"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1005)
                     /locus_tag="VMZ53_31490"
     CDS             complement(25..1005)
                     /locus_tag="VMZ53_31490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUS33082.1"
                     /translation="MASTALSVKGIVKRYGNHVAVDGVSFEIPRGVIYGILGPNGAGK
                     STTLRMVNDIIAPDAGEIRILDGLPPGSAAARQIGYLPEERGLYPKMKVADMIIFMGE
                     LRGLSHGEAKKRAHKWLERLGLGAWTKNKVQDLSKGMAQKVQFAIALIHEPQLVILDE
                     PWSGLDPINAEVLREVVEEIKASGRTVLFSTHGMEQAEKICDAVCIIARGKKVLDGRL
                     RDIKRDSVAEGVIALGFSDEASKETAAGPLEDHKLVTARRPPRPGEHADCEVILAEGV
                     KPQQLLAILLNHDVGVRLFEVVVPTLHDIFVQKVGNAAIAVRREEDDAGA"
     gene            complement(1006..1974)
                     /locus_tag="VMZ53_31495"
     CDS             complement(1006..1974)
                     /locus_tag="VMZ53_31495"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HUS33083.1"
                     /translation="MTTGPRHLVSIADLDAAEIVRILDHGDRYWSLADEPIKKLTSLR
                     GRTVINLFYESSTRTRTSFELAAKRLSAEAINIAASSSSTTKGETLLDTGRNLQAMHP
                     DAIVVRHAFGGAAYLLAKTANCAVVNAGDGQHEHPTQALLDAATIRRHKGNVSNLEVA
                     IVGDIQHSRVARSNILCLSKLGARVRLVAPHTLMPRGIELIGGELTRERVRLCHRLED
                     GLDGADVIMMLRVQHERFEGEASRFPNTRELSRTFGLSERTLAYAKNDAIVMHPGPIN
                     RGVEVSPAVADGPRAVILQQVSWGVAIRMAVLERAVLGGAPAEQAA"
     gene            2014..>2654
                     /locus_tag="VMZ53_31500"
     CDS             2014..>2654
                     /locus_tag="VMZ53_31500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33084.1"
                     /translation="MSGAKVNRIGTIVGDRFRVDEMIGKGAMADVYRALDLQTQAFVA
                     LKILRTTHVTDAVSLARFNREAEVQERIRHRNVAALLATGVTDTKEPYLAVELLRGKS
                     LRSVIKAEKSVAPRRAASYAWQALQGLAAVHAIGVLHRDLKPANIMLEPSPGPVERVV
                     LIDFGFATFEGSAKLTLQGTVVGSLTYIGPERLRGEGADARTDIYAIGVILFEL"
BASE COUNT          423 a          889 c          881 g          461 t
ORIGIN      
        1 tcgcgcttcg cgatgaccca ggtatcacgc accggcgtca tcctcttcgc ggcgcacggc
       61 gatcgctgcg ttgccgacct tctgcacgaa gatgtcgtgc agcgtcggga cgacgacctc
      121 gaacaggcgc acgccgacgt cgtggttgag caagatcgcg agcagctgct gcggcttcac
      181 gccctcggcg aggatcactt cgcaatcggc gtgctcgccc gggcgcggtg gacggcgcgc
      241 ggtcacgagc ttgtgatcct cgagtgggcc agccgctgtc tccttcgagg cctcgtcgga
      301 gaagccgagc gcgatcacgc cttcggcgac ggagtcgcgc ttgatgtccc gcagcctgcc
      361 gtcgagtacc ttcttgccgc gcgcgatgat gcagaccgcg tcgcagatct tctcggcttg
      421 ttccatgccg tgcgtcgaga acaacaccgt gcggcccgac gctttgatct cttcgacgac
      481 ctcgcgcagc acctcggcgt tgatcggatc gagaccgctc catggctcgt cgaggatcac
      541 gagctgcggc tcgtgaatga gcgcgatcgc gaactggacc ttttgggcca tgcccttcga
      601 gaggtcctgc accttgttct tcgtccacgc gccgaggccg agacgctcga gccatttgtg
      661 tgcgcgcttc ttcgcttcgc cgtgagagag gccgcgcagc tcgcccatga agatgatcat
      721 gtcggcgacc ttcatcttcg ggtacaggcc gcgctcctcg ggcagatagc cgatctggcg
      781 cgcagccgcg gatcccggcg ggagcccatc gaggatccgg atctcgccgg cgtccggcgc
      841 gatgatgtcg ttgaccatgc gcagcgtcgt cgacttgcca gcgccgttcg gaccgaggat
      901 gccgtagatg acaccgcgcg ggatctcgaa cgagacgccg tcgacggcga cgtgattgcc
      961 gtagcgcttg acgatgccct tcacggacag cgcggtcgac gccatttacg cagcctgttc
     1021 tgcgggcgct ccgcccagta ctgcacgctc gagcaccgcc atgcgaatcg cgacgcccca
     1081 gctgacctgt tgcaggatca cggcgcgcgg cccgtcagcg acggcgggag atacctcgac
     1141 accgcgattg atcggcccgg ggtgcatcac gatcgcatcg ttcttcgcgt acgcgagcgt
     1201 gcgctccgag agaccaaacg tccgcgacag ctcgcgcgtg ttcgggaagc gcgacgcctc
     1261 accctcgaag cgctcgtgct ggacgcgcaa catcatgatg acgtccgcac cgtcgagccc
     1321 atcttcgagg cgatgacaca gccgcacgcg ctcgcgtgtc agctcgccgc cgatgagctc
     1381 gatcccgcgc ggcatcagcg tgtggggcgc gacgaggcga acgcgcgcgc cgagcttcga
     1441 gaggcagagg atgttcgaac gcgcgacgcg cgagtgctgg atgtcgccga caatcgcgac
     1501 ctcgaggttg ctcacgttgc ccttgtggcg gcggatcgtc gcggcgtcga ggagggcttg
     1561 cgtcgggtgc tcgtgctggc cgtcacctgc attgaccacc gcgcagttcg cagtcttcgc
     1621 gagcaggtag gccgcgccgc cgaatgcgtg ccgaacgacg atcgcgtcgg ggtgcatggc
     1681 ctggaggttg cggcccgtgt cgagcagggt ctcgcccttc gtcgtcgacg acgagctcgc
     1741 ggcgatgttg atcgcctcgg ccgacagccg cttggcagcg agctcgaacg atgtccgcgt
     1801 ccgcgtcgag ctctcgtaga acagattgat gaccgtgcga ccgcggaggc tcgtcagctt
     1861 cttgatcggc tcgtccgcga gcgaccagta gcgatcaccg tggtcgagga tgcggacgat
     1921 ctcagcggcg tcgaggtcgg cgatcgagac caagtgcctg gggccggtcg tcacgctctc
     1981 catatagcag atcaccggtg ttatcgtggc gcggtgtccg gagcgaaagt gaaccggatc
     2041 ggcacgatcg tcggggatcg gttccgcgtc gacgagatga tcggtaaagg cgcgatggcc
     2101 gacgtctatc gagccctcga tctgcagacg caggcgttcg tggccctcaa gatcctgcgt
     2161 acgacgcacg tcaccgacgc cgtttcgctc gcccgcttca atcgcgaagc cgaggtccag
     2221 gagcgcatcc ggcaccgcaa cgtcgccgcg ctcctcgcga cgggagttac cgatacgaag
     2281 gagccgtacc tcgcggtcga gctgctccgc ggcaagtcac tgcgcagcgt gatcaaggcc
     2341 gagaagtccg tcgcgccgcg ccgtgccgcg agctacgcgt ggcaagcgct gcaaggcctc
     2401 gccgccgtcc acgcgatcgg cgtgctgcat cgcgatctca agccggcgaa catcatgctc
     2461 gagccgtcgc ccggcccggt cgagcgcgtc gtgctgatcg acttcggctt cgcgaccttc
     2521 gaaggcagcg cgaagctcac gctgcaaggc accgtcgtcg gctcgctgac gtacatcggg
     2581 cccgagcgcc tccgcggcga aggagccgac gcacgcacgg acatctatgc gatcggcgtg
     2641 atcctcttcg agct
//
LOCUS       DATLBZ010000639         5654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8804041, whole genome shotgun sequence.
ACCESSION   DATLBZ010000639 DATLBZ010000000
VERSION     DATLBZ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8804041"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VMZ53_31505"
     CDS             <1..409
                     /locus_tag="VMZ53_31505"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HUS33085.1"
                     /translation="GFPESALLALSLGCDMVNVAREAMLSIGCIQAQRCHTGHCPTGV
                     ATQNRWLMRGLDPTLKSARLANYIATLRKELSSLAAACGHTHPALVPLDQIDIVESAT
                     LTRSARLVYGYQPGWGLPPAHEVESATKAVAAA"
     gene            442..1008
                     /gene="orn"
                     /locus_tag="VMZ53_31510"
     CDS             442..1008
                     /gene="orn"
                     /locus_tag="VMZ53_31510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HUS33086.1"
                     /translation="MADVLVWLDMEMTGLDPERERIIEVATIITDGQLTEVATGPEVV
                     IHQPDEILGAMDDWNKTHHGASGLTERVKASTISEADAEAQTIAFINQHCGAKERPVL
                     AGNSIHQDRRFIHRYMPALDKRLHYRMVDVSTIKELARRWYPAITSKQPPKKETHRAL
                     DDIRESIDELRFYKQHLFCPPPTDLKPV"
     gene            1148..2794
                     /locus_tag="VMZ53_31515"
     CDS             1148..2794
                     /locus_tag="VMZ53_31515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33087.1"
                     /translation="MSVARTPDMRTLATAALAASLLSTSVLAGCAGSGDEGDPEDTVF
                     VDENKADNFYSTSAAEYVITGKSTVVLDASMATASDADRLAAAKKLIELKQIAIAWFL
                     TEYFKSKEHEEANYSFGGFSGIAKDGEFVDLDVAPRADKLTYDFTFHQLAAAGKGLMT
                     QLPIRQVNGMPSFDLEIGKPSNAELAQLETNHEWYRNAPWSGWNPSMVSADKKEAMTF
                     GIKPEKESSDGFFNLEKYTADGVLDIDVFFGWDYHSEYHIKHSQAFFTWLKGQGFTAP
                     VASWDAMTKDTGAFTKTVKADGKNVKVEVRFYFGKPGTATDPDTDAGGKVLEDLARAS
                     LAKRDVVMYSGHSGPFYGFALANWKKTEEGDLDDADIKAMQLPADKDQLVLANGCETY
                     QIGEAFRTNPAKAGKNIDVITTMSFSNAGTPTIVEATVEALLARDASNNLRPQPVSTL
                     LTKIDSDSWYFKPMYGFHGIDDNPKVVPWARTANFGKACTTNSQCGGPGNLCVSTGSA
                     KVCTAACASDGGCGTGMTCKPVASSSTNTIYGKACAKTVN"
     gene            2941..4335
                     /gene="glgC"
                     /locus_tag="VMZ53_31520"
     CDS             2941..4335
                     /gene="glgC"
                     /locus_tag="VMZ53_31520"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HUS33088.1"
                     /translation="MKDTLVMILAGGKGSRLGPLTVHRSKPGVPFAGRYRIIDFVLSN
                     IINSGYRRVYVLTQYMSSSLIKHLSRNWRLSGFGEYIEVVPAQMRLGEFWYRGTADAV
                     YQNLNLVRDARAEHVAIFGGDHIYKMAVDQMEEQHRDRAADLTVAAFPVPTKEASAFG
                     IIDVDERGRIVGFIEKPKNPPEMPGRPGWSLVSMGNYIFRREVLERVFQDESGRPRHD
                     FGKDIIPRLVADGARVFVYDFAQNKVPGDPEGVEPYWRDVGTIDSYFTSNMELRARVP
                     ALDLYNRRWRIRSAQRDYPPARFVRAGEGLPFCEVDDSLICEGSIISSATVCESVLGY
                     DCFVHAGATVKDSVILSGCDIGRGARLNRVLLDKNCKIEPGCIIGEDPEEDAKRFPFM
                     TESGIVVVPKGTLVPANGPCVLANDVAELMSNDPELADKLEPGTFVVSMQSRHSYESA
                     GPRFKKFAGDLVER"
     gene            4390..>5654
                     /locus_tag="VMZ53_31525"
     CDS             4390..>5654
                     /locus_tag="VMZ53_31525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HUS33089.1"
                     /translation="MRIVHVSSEVAPFAQSGGLADVLAGLPTALAATHRLDVAVVCPL
                     YRGVEAKLSAAGAKLDAGTPISVTLGPHVFAASLRTARIADVTYGFVDCAPLYDRPGT
                     LYGPTPTSDFPDNHLRFGVLGKVALEHGAKLVGGKPIDVLHVHDWQGSPAAIFAKMAN
                     APLSIVTTIHNLAFRGIFPKSVMTDLAIPWSMFTHEQLEFYDQLSLLKGAMAYSDVVT
                     TVSPTYAHEILTPPFGEALDGFLQWNVKRLVGIVNGIDARGWDPATDTALAAPFSATN
                     PAGKAQCRAALAAELGLALDDSTPLIATISRMAHQKGLDLVADIAPEMHRLGAKLVVL
                     GSGDPDLEARFRYLASSFADTIAVRIGFDINLSRRIYAGSDLFVMPSRFEPCGLGQLY
                     AMRYGSVPIVHAVGGLRDTVTDPGSYDALA"
BASE COUNT         1065 a         2006 c         1682 g          901 t
ORIGIN      
        1 cggcttcccc gaatccgcgc tgctcgcgct ctccctcggt tgcgacatgg tgaacgtcgc
       61 gcgcgaagcg atgctctcga tcggctgcat ccaagcgcag cgctgccaca cgggccactg
      121 ccccacgggc gtcgccacgc agaaccgctg gctgatgcgc ggcctcgatc caacgctcaa
      181 gtccgcccgc ctcgcgaact acatcgcgac gcttcgcaaa gagctctcct cgctcgccgc
      241 tgcctgcggc cacacgcacc ctgccctcgt gccgctcgat caaatcgaca tcgtcgagag
      301 cgccacgctc acgcgctccg ctcgcctcgt ctacggctat cagcccggct ggggcctgcc
      361 ccccgcgcac gaggtcgagt cggcgacgaa agccgtcgca gccgcctaga ttccgcgcgc
      421 gcgcccaagt agggtcgcgc catggccgac gttctggtct ggctcgatat ggagatgacc
      481 gggctcgatc ccgagcgcga gcgcatcatc gaggtcgcaa cgatcatcac cgacggtcaa
      541 ctgaccgagg ttgcaaccgg tcccgaagtc gtcatccacc agcccgacga aatcctgggc
      601 gcaatggacg actggaacaa gacgcaccac ggcgcgtccg gcctcaccga acgcgtcaaa
      661 gcctcgacga tcagcgaagc cgacgccgaa gcccaaacga tcgcgttcat caaccagcac
      721 tgcggcgcca aagagcggcc ggtcctcgcc ggcaactcga tccaccaaga ccgtcgcttc
      781 atccatcgct acatgcccgc gctcgacaag cgcctgcact accgaatggt cgacgtctcg
      841 acgatcaaag agctcgcccg ccgctggtac cccgcaatca catcgaaaca gccgcccaaa
      901 aaagagacgc accgggccct cgatgacatc cgcgaatcga tcgacgagct gcggttctac
      961 aagcagcacc tcttctgccc gccgcccacc gacctaaaac cggtctgacc actaagggac
     1021 ggacgcaact tccgcaacct ccgcaaccgc gagggacgga gcgagggacg aagcgcaact
     1081 tccgagagcc ggctacgcga gtcgccactg gctccaactg tgcgaaccga caggcggcga
     1141 tcggggcatg tccgttgctc ggacaccgga catgcgtacg ctcgcgactg ctgcactagc
     1201 cgcctccctc ctctcgacgt ccgtcctggc gggctgcgcc ggctccggcg acgagggcga
     1261 tcccgaggac acggtcttcg tcgacgagaa caaggccgac aacttctaca gcacgtcggc
     1321 tgccgagtac gtcatcaccg gcaagtcaac ggtcgtcctc gatgcatcga tggcgacggc
     1381 ctccgacgca gaccgcctcg cggctgccaa gaagctcatc gagctcaagc agatcgcaat
     1441 cgcgtggttc ctcaccgagt acttcaagag caaggagcac gaggaggcga actactcgtt
     1501 cggcggcttc tcgggcatcg cgaaggacgg cgagttcgtc gacctcgatg tcgcgccgcg
     1561 cgcagacaag ctcacgtacg acttcacgtt ccatcagctc gcggcggccg gcaaaggcct
     1621 catgacgcag ctgccgattc gccaggtcaa cggcatgccg tcgttcgatc tcgagattgg
     1681 caagccgtcg aacgccgagc tcgcgcagct cgagacgaac cacgagtggt atcgcaacgc
     1741 gccgtggagc ggctggaacc cgtcgatggt gtccgccgac aagaaggaag cgatgacctt
     1801 cggcatcaag ccggagaaag agtcgagcga cggcttcttc aacctcgaga agtacaccgc
     1861 ggacggcgtg ctcgacatcg acgtcttctt cggctgggac taccacagcg agtatcacat
     1921 caagcattca caggcgttct tcacgtggct caagggccag ggcttcacgg cgccggtcgc
     1981 gagctgggac gcgatgacga aggacaccgg tgcattcacg aagaccgtga aggccgacgg
     2041 caagaacgtg aaggtggaag tccgcttcta cttcggcaag ccgggtaccg cgaccgatcc
     2101 ggatacggac gcgggcggca aggtgctcga ggacctcgcg cgcgcctcac tcgcgaagcg
     2161 tgacgtcgtc atgtactccg gccactcggg cccgttctac ggcttcgcgc tcgctaactg
     2221 gaagaagacc gaggaaggcg acctcgacga cgccgacatc aaggccatgc agctccccgc
     2281 cgacaaagat cagctcgtgc tcgcgaacgg ctgcgagacg taccaaatcg gcgaagcgtt
     2341 ccgcacgaac cccgcgaagg ccggcaagaa catcgacgtc atcacgacga tgtcgttctc
     2401 gaacgccggt acgccgacca tcgtcgaagc caccgtcgaa gcgctcctcg cgcgcgacgc
     2461 cagcaacaac ctgcgcccgc agccggtctc gacgctcctg accaagatcg acagcgactc
     2521 ttggtacttc aagccgatgt acggtttcca tggcatcgac gacaacccga aggtcgtccc
     2581 gtgggcgcgt actgccaact tcggcaaggc gtgcacgacg aactcgcaat gcggcggtcc
     2641 cggcaacctg tgcgtgtcga ccggctcggc gaaggtctgc accgcggcct gcgcgtcgga
     2701 cggcggctgc ggcaccggca tgacgtgtaa accggtcgcg agcagctcga cgaacaccat
     2761 ctacggcaag gcctgcgcga agaccgtgaa ctgagcgcgc cagccacgaa ctgacgtcat
     2821 cgcatagctc cgctcgccgc cgatctggcg cggttcggag cgcaaggtct gcgcaaggtc
     2881 gcgatatcca ggcgcgctcc cgtcgcctgc cgcagacctc ttgctaggat gatcgcgaga
     2941 atgaaagaca cgctcgtcat gatcctggcg ggtggcaaag gctcccgcct cggcccgctc
     3001 acggtccacc gctccaaacc cggtgtcccg ttcgccggtc gctaccggat catcgacttc
     3061 gtcctctcga acatcatcaa ctcgggctac cgacgcgtct acgtgctcac gcagtacatg
     3121 tcgtcgagcc tgatcaagca tctctcgcgc aactggcgcc tctcgggttt cggcgaatac
     3181 atcgaggttg tccccgcgca gatgcgactc ggcgagttct ggtaccgcgg caccgcagac
     3241 gccgtgtatc agaacctcaa tctcgtgcgc gacgcgcgcg ccgagcatgt cgcgatcttc
     3301 ggcggcgacc acatctacaa gatggccgtc gatcagatgg aagaacagca ccgcgatcgc
     3361 gcagccgatc tgacggtcgc tgcgttcccg gttccaacga aagaggcgag cgcgttcggc
     3421 atcatcgacg tcgacgagcg cggccgcatc gtcggcttca tcgagaagcc gaagaacccg
     3481 cccgagatgc ctggccgccc cggctggtca ctcgtctcga tgggcaacta cattttccga
     3541 cgcgaagttc tcgagcgcgt gttccaggac gagagcggtc ggccgcgcca cgatttcggc
     3601 aaggacatca tcccgcgtct cgtcgccgat ggcgcgcgcg tgttcgtcta cgacttcgcg
     3661 cagaacaaag tgccgggcga cccggaaggc gtcgagccgt actggcgcga tgtcggaacg
     3721 atcgactcgt acttcacgtc gaacatggag ctgcgtgcac gcgtgcccgc gctcgatctc
     3781 tacaaccggc gttggcgcat tcgcagcgca cagcgcgatt acccgcccgc tcgcttcgtg
     3841 cgtgccggcg agggcctgcc gttttgcgaa gtcgatgact cgctgatctg cgagggctcg
     3901 atcatctcgt cggcgacggt ctgcgaatcc gtgctcggct acgactgctt tgtccacgcg
     3961 ggcgccacgg tcaaagactc cgtcatcctg tcgggctgcg atatcggccg cggcgcgcgt
     4021 ctcaaccgcg tgctcctcga caagaactgc aagatcgagc cgggctgcat catcggcgaa
     4081 gaccccgagg aagacgcgaa gcggttcccc ttcatgaccg aatcgggaat cgttgtcgtt
     4141 cccaaaggca cgctcgttcc agcaaatggc ccgtgcgtcc ttgccaacga cgtcgccgag
     4201 ctcatgagca acgaccccga gctcgccgac aagctcgagc ctggaacgtt cgtcgtctcg
     4261 atgcagagcc gccacagcta cgaatcggcg ggcccacgct tcaagaagtt cgccggcgat
     4321 ctagtcgagc gctaaccgcg cggcggattc gcccggccgt cggagggtgc gctacaaaca
     4381 agaagccgta tgcgcatcgt tcacgtttcg agcgaggtcg cgccattcgc gcaatccggc
     4441 ggcctcgccg atgtcctcgc cggcctccca accgcgctcg cggccacgca tcgcctcgac
     4501 gtagcagtcg tgtgcccact gtaccgaggc gtcgaagcga agctctccgc cgctggcgcg
     4561 aagctcgatg cgggcacgcc catctccgtc acgctcggcc cgcacgtgtt cgctgcgtcg
     4621 ctgcgaacgg ctcggatcgc cgacgtcacg tacggcttcg tcgactgcgc gccgctctac
     4681 gaccgccccg gaacgctgta cggcccgacg ccgacctccg acttccccga caaccacctc
     4741 cgcttcggcg tcctcggcaa agtcgcgctc gagcacggcg caaagctcgt cggtggcaaa
     4801 ccgatcgacg tcctccatgt ccacgactgg cagggctcac cagcggcgat cttcgcgaag
     4861 atggcgaacg cgccgctctc gatcgtcacg acgattcaca acctcgcgtt ccgcggaatc
     4921 ttcccgaagt ccgtgatgac cgacctcgcg attccgtggt cgatgttcac gcacgagcaa
     4981 ctcgagttct acgatcagct gtcgctgctc aaaggcgcga tggcgtactc ggacgtcgtc
     5041 acgaccgtga gcccgacgta cgcgcacgag atcctcacgc cgccgttcgg cgaagcgctc
     5101 gacggtttct tgcagtggaa cgtcaagcgc ctcgtcggca tcgtcaacgg catcgacgca
     5161 cgcggctggg atccggcgac ggacaccgcg ctcgcagcgc cattcagcgc caccaacccg
     5221 gccggcaaag cgcagtgccg cgccgcgctc gcagccgagc tcggtctcgc gctggacgac
     5281 tcgacgccgc tcatcgccac gatctcgcgc atggcgcacc aaaaaggcct cgacctcgtc
     5341 gccgacatcg cgcccgagat gcatcgcctc ggcgccaagc tggtggtgct cggctcgggc
     5401 gacccggatc tcgaagcgcg cttccgctac ctcgcgtcgt cgttcgccga caccatcgcc
     5461 gtgcgcatcg gcttcgatat caatctctcg cgtcgcatct acgcaggcag cgacctgttc
     5521 gtgatgccgt cgcgcttcga gccatgcggc ctcggccagc tctacgccat gcgctacggc
     5581 tccgtgccga tcgtccacgc ggtcggcggc cttcgcgaca cggtcaccga tccaggaagc
     5641 tacgacgcgc tcgc
//
LOCUS       DATLBZ010000640        12718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_11034446, whole genome shotgun sequence.
ACCESSION   DATLBZ010000640 DATLBZ010000000
VERSION     DATLBZ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11034446"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(465..2078)
                     /locus_tag="VMZ53_31530"
     CDS             complement(465..2078)
                     /locus_tag="VMZ53_31530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33090.1"
                     /translation="MKRCATCQSEYPDDANWCRFDGGKLETVAAVPPAAAAAAPGPQL
                     PAQPSALTMGWDVPNVPMTFTGTPPPAVQQKLAQVSSTKPAAAPPAAAAAPPRAPNAP
                     SALTMGWDAPADPTETPAATSPSKLAAAAATAAPAANALRAPNAPSALTMGWDAQPEP
                     VVSPSQLAAAAAAKSQPQAPTPGALTMGWDVPAVPTTFTGTPPAPPSSPAGASVPSTS
                     PSQLAAAAAKPQPHLSTKPGSLTMGWDAMPDADPASTQPTMQAPLPAGAQPSAFAKSP
                     SAMQSPSPLQSPPATSAPKSSPQSPSQKPGALTMGWDAMPDPEPATATAPSAASPLSP
                     AALAAKSATASSSIGGPSAGPAASAASAAAANHAPAATLLGMQAPWAQQKINPVPANP
                     RDGAQSGVPQQQMRDTPSQPHAQPNYAGVPQQQTRDTPSQPNPAPYGQPQHAAAPSYA
                     PHPARDAPSAPHAAAPHGHAGYGHAPPMQAPAAIPATSNSSPETTLRTRPNADDSALD
                     RPPRNMLPVYAMLGIAVIAIIVVVLAFVL"
     gene            complement(2092..3591)
                     /locus_tag="VMZ53_31535"
     CDS             complement(2092..3591)
                     /locus_tag="VMZ53_31535"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HUS33091.1"
                     /translation="MITRRGFMIGGAASLVACRVAPVTRIARPQHTHGLQIGDVSTER
                     ALVWARCSEPARLELEWDTTPRFANARRTATPTAVTPDSDFAATVPLDKLPSAQRVFV
                     RARFTREADRGTSDWATASFATPGTAPFRVAWTGDTCGQGFGRNPDWGGLRGYAAVRD
                     AKPAFLIHSGDLIYADNPIESEKRTLDGKLWRNISNERVARVAQDLDDFRARFAYNLE
                     DDHVRALAAEVPILAQWDDHETHNNWFPSQTLDDDRYTKLRDASRLAAFAQRAMREWT
                     PMPQGPVHRVVSYGPLLDVIVLDCRSFRTPNDAGAGTAMLGAAQAAWFVDAVSRSRAT
                     WKIIACDQPLGLIIPDGPRDDRFEGYGNVAGPPLGREIELAAMLAELKARNAKNFVFI
                     TADVHYAAAHRFDPSRASVPSAVSFDPFWELVAGPIHAGTFGPNELDPSLGPELVFQW
                     APPPGTGNLAPWDGLQSFGTIDVSADALAISLLSIDGKKRFAVEVTRAA"
     gene            3631..4836
                     /locus_tag="VMZ53_31540"
     CDS             3631..4836
                     /locus_tag="VMZ53_31540"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HUS33092.1"
                     /translation="MSSRPLAIALCILGACSHGDPPDISGLSDQVAVVGQQFVLELQG
                     TDPDGDDLEYGVRSDLELESRATISKTPTGNGVFRWTPLAADVGLHTFDFKVSDGSND
                     TTVTIQIDVRATSAGVPVFREPLSSGRVVNLSAEPCINVPIVIEDQDSAQVTIDEEEP
                     KIVGATLTQSDGLTGNWKWCPTPAQVADSDRYTLVLSADDGDNPKVLKPYVIVLGGGG
                     GPKLVINEVDYDNVGTDNAEYIEIYNGSSTTTSLAGLSVILVNGATGDEYATIELGPV
                     GSLTAGQYLVIGGPAVTVPTAAKKLDPLWTQDQVQNGAPDGVALVDTVGPTLLDALSY
                     EGAVTAANLTGFASSVSLVEGTALATGSADSNTATATLCRKPNGADTNNASADWKVCT
                     TRTVGTANN"
     gene            complement(4833..6446)
                     /locus_tag="VMZ53_31545"
     CDS             complement(4833..6446)
                     /locus_tag="VMZ53_31545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33093.1"
                     /translation="MGDPRQRFEKLRGALEALAGARTSKDVARVAVETGRDAVDAIAA
                     TIWLHDPSDGLFKVAASHNVAEDYLAPFRVIRPDSRLPVVRVTTTRTAMFVGNEQELA
                     QLAPEMYPEAHNAGRIQPFAVLPLATEERALGAIAFTFSGRFHDYSDDERELLFSLTR
                     ACEQALERARLLEAEAEARRVAEEANRRKDEFLAMLGHELRNPLAAMSAAVDLIKLRE
                     ATLSRELTILDRHLASLVHMVSDLLDVSRVTLGKVDLERTSCDVKRSIEQALESVQSE
                     IDARGHTVSVDVAADLAVNADPERLTQVFVNLLANAVTYTPAGGRIHVTARDDVDDIV
                     IDVRDNGVGIAATLVPTIFDAFVQGPRSLDRKPGGLGIGLTLVKQLVELHGGRVSVVS
                     EGEDKGSTFSVSWPRAVGPTRTVKMPALGKVRPLRVLVVDHEVDGAHAFARVIEGMGH
                     EVVVVHDAPDALAAGERFSAEIVFLDLAVGGYDLAKQLRAVPAFKAARVVALGSNGAR
                     DESVDGVTAHIAKPLDVHTLAELLSVHAS"
     gene            complement(6643..7326)
                     /locus_tag="VMZ53_31550"
     CDS             complement(6643..7326)
                     /locus_tag="VMZ53_31550"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HUS33094.1"
                     /translation="MCRMFGMVATHAVSPRELLHDAPRSLRALSHEHGDGWGIALASG
                     DWQIHKSTERAAHCTSFSAIADTAARVVVAHVRKKTVGDLAIANTHPFRRGNLVFAHN
                     GTVASLAALSDHIALARLAEIEGDTDSERLFAFVLTHIDAAGDVERGVVRAIRALHSL
                     GEIGSVNFLLASDSRVYAYRQGRTLFTLSRTGVAMVASERLTDEDWLEVPDRGLVILG
                     RSVLEVAML"
     gene            complement(7378..7773)
                     /locus_tag="VMZ53_31555"
     CDS             complement(7378..7773)
                     /locus_tag="VMZ53_31555"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HUS33095.1"
                     /translation="MTKHHGSCHCGAVTFEATFELKTASRCNCTICTKLMWTGVIMKP
                     DAFRLLSGEGDLSGYVWGGKISTRHFCKHCGVQCFGKGHLDVLGGDFVSVNVNALEGV
                     EVHEVPLIHFDGRHDNWQAGARDKPWPIA"
     gene            complement(7810..10899)
                     /locus_tag="VMZ53_31560"
     CDS             complement(7810..10899)
                     /locus_tag="VMZ53_31560"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33096.1"
                     /translation="MDSAETGFPMTEAVRDVSHGVGLPPHTPRPHWVQFYETDNFLGD
                     AVSAFFAEGMKAGEPGVIIATAPHREMFVDQMTARGINVQREIDAGRLVMLDAAETLG
                     AFMGEAAPIREKFFATVGPVLDGLCRLASARQVRAFGEMVDILWRSGLRTAALELEEL
                     WNELRIRYDFALLCAYVMDSFYKQNGVPAICATHSHVLPPENVGAVSTALSANVQSLV
                     HEIAKRTELEITLRESINNVRRSEEETRRAKEDLEDILENAPIPIHRVDRKGVIRWAN
                     NAELDLLGLARHEYVGHHISEFHADEDKIKDILRRLLAGEVLQDYEAPLRAKDGTIRW
                     VQISSNMQQDNDRGTTRCFCRDVTAQKHAERDRDAIFRVTKMLGAELDLDRLLAKLIS
                     EVKRLTGAEKVCFLEGSRNDELAAMPSYLEVPLVAPSGELLGALALGHSQPAMFMERE
                     ENLLTAIASQAAGALSNARVYEAERSARANAERAERNVTLLQGVTSALSRAVSAEEAC
                     RVVVHETRAMLDASAACVVMLDRSGTKTERILVEGDYDDSHMSELQDQLLDSHRPAAE
                     AARSGKLVWIVGATTIDARFPELREARASLGTLTWGAIPIVFEGRTHGAIGFRCMHER
                     SLTPEEETLLLAVGRQCGQALERARLHEATQAARREAEQASRAKDEFLAMLGHELRNP
                     LSPILTAVQLMRVRGDQTSVREQSVIERQVTHLIHLVDDLLDISRITRGKVQLDKHPH
                     MLASLVTKAVEIASPLCEENRHHVTVDIPVETIWLEADETRLCQVFTNLVTNAAKYTP
                     RGGRIDIRAKRNGQHVRISVKDNGVGITPELLPKVFDLFVQGERRTHRSQGGLGIGLA
                     IVKNLVTLHGGTVKVISDGVNRGSEFIVELPVVDMQAKPNLERSVRQGVQVTPRKILV
                     VDDNEDAGSLLGEMLSSLGHEVVVALDGPRALEVLERFTPEIGILDIGLPVMDGYELA
                     SALRTRLGGGLRLMAVTGYGQEQDKQRTQQAGFEAHFVKPVGLGKVLAAIEVAAKQT"
     gene            complement(10983..11822)
                     /locus_tag="VMZ53_31565"
     CDS             complement(10983..11822)
                     /locus_tag="VMZ53_31565"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HUS33097.1"
                     /translation="MTRCLVLVLALALCACRGSGGDDSMALDDAPTGGGDGSGSATFT
                     LPPLNGALDYQLGGAYTPPAGVTIVSRDREAAPASGVYNICYVNGFQIQQAEESFWTG
                     MHADLILRDGNGNPVIDQDWNEMLIDVSTPAKQQAVAAIVGEWIAGCATAGFNAVEID
                     NLDSYSRSGGRLTEDNAVAAMKLFAQAAHNASLAIAQKNSSEIVSRKAEMGTDFVVSE
                     ECNRYAECDAFRAGYGDHVLVIEYRQQDFNTGCAAYPQLSIVLRDLNLVTPSQPGYVF
                     DGC"
     gene            11903..12526
                     /locus_tag="VMZ53_31570"
     CDS             11903..12526
                     /locus_tag="VMZ53_31570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33098.1"
                     /translation="MLRATTLLLCIVGSVTVVRAAPDPAPIDRTELRRALAEERQVNL
                     ARFHDYRKKRVYPHNTYEPGMLNVWKDNDDHLCAVATLVHLDGHDDIVDTVATQENFV
                     KTAELTDGPLIDWMLTSGLTHEEIVMIQQPSAADIAAMDAEYRAEQRRIKRWLAREDA
                     RLAKVYKTVEKTLTAPRIADAGLDLATARLAQHPELAKALLAKHPAH"
BASE COUNT         2107 a         4138 c         4299 g         2174 t
ORIGIN      
        1 gtcgatcgtc gtcgcgattc cgtgcccacg tgcgcacgac cacccggtcg tcgtcgcaac
       61 cgtgcccccg tgggcgcgcc gaggtcggtc gtcgtcgcga cagtccgacg tgggcgcgac
      121 cacgccggtc gtcgtcgcga cgccgcgggg cgtgacgcag cgcgcgcatc cgcgcgatca
      181 acgtgcgcct gcgtgcccac gtgggcacga ccacctggtc gtcgtcgcga tcccgtgccc
      241 acgtcggcat gacagcgtcg atcgtcgttg cgattccgtg cccacgtggg cgcgaccacc
      301 cggtcgccgc gattctgcac cttggctcga cgtcgatctc gtcgcgatgc catcccacgc
      361 gggaacgacc tcgcgatcgt gcgtcgcgat tccgtgccca cgtgcccacg tgggcacgat
      421 gatcggtcga gcgcggggca caaggccgct gggcccgtgg cgcgctagag cacgaacgcg
      481 agcacgacga cgatgatcgc gatcacggcg atgccgagca tcgcgtagac gggcagcatg
      541 ttgcgcggcg ggcggtcgag cgcgctgtcg tcggcgttgg ggcgcgtgcg caacgttgtt
      601 tcgggcgagc tgttgctcgt tgcggggatt gcggcgggtg cttgcatcgg cggcgcgtgg
      661 ccgtagccgg cgtggccgtg tggcgctgcg gcgtgcggag cggacggtgc gtcgcgcgct
      721 gggtgcggcg cgtacgacgg cgcggcggcg tgttgcggct gaccgtacgg cgcgggattt
      781 ggctgtgatg gtgtgtcgcg cgtttgctgc tgcgggacgc cggcgtagtt cggttgagcg
      841 tgcggctgtg agggcgtgtc gcgcatttgc tgctgcggga cgccggattg cgcgccgtcg
      901 cgcggattcg cagggactgg attgatcttt tgctgcgccc atggcgcctg catgccgaga
      961 agcgtcgcgg cgggcgcatg gttcgcggcg gcggcagagg ccgcagaggc ggcggggccg
     1021 gccgacgggc caccgatgct cgaggaagcg gtcgcggatt ttgcggcgag cgcggccggc
     1081 gagagcggcg atgcagccga aggtgctgtc gcggtggctg gctcgggatc cggcatcgca
     1141 tcccagccca tcgtgagcgc gcctggcttt tgcgacggcg attgcggcga tgacttcggc
     1201 gcggacgtcg ccggcggtga ttgcagcggt gatggtgatt gcatcgccga gggcgatttc
     1261 gcgaacgcgg acggctgggc acccgcaggc agcggcgctt gcatcgttgg ttgcgtcgat
     1321 gcggggtcgg cgtccggcat cgcgtcccag cccatcgtca gagagcccgg cttcgtcgag
     1381 agatggggct gcggtttcgc ggcagcagcg gcgagctgcg atggagacgt cgacggaacc
     1441 gacgcgcccg ctggcgacga cggcggcgcg ggcggcgtgc ccgtgaacgt cgtcggcacc
     1501 gcgggcacat cccagcccat cgtcagcgcg cccggcgtcg gcgcttgcgg ctgcgacttc
     1561 gccgcggcag cagcggcgag ctgcgacggc gacacgaccg gctcgggctg cgcatcccaa
     1621 cccatcgtca gagcgctcgg cgcgttcggc gcgcgaagtg cgttggcagc cggcgcggcg
     1681 gtggcagcgg cggcagcgag cttcgatggc gatgttgcgg ctggcgtctc cgttggatcg
     1741 gccggcgcgt cccagcccat cgtgagcgcg cttggcgcat tcggcgcgcg aggtggcgcg
     1801 gcggcggcag ccgggggagc ggcagccggc ttcgtcgacg atacctgcgc gagcttttgc
     1861 tgaactgcgg gcggcggcgt gcccgtgaat gtcatcggca cattcggaac atcccagccc
     1921 atcgtcagcg cagacggctg cgcgggaagc tgcggaccgg gcgcggcggc ggcggcggcg
     1981 ggcgggaccg cggcgaccgt ctcgagctta ccgccatcga agcgacacca atttgcgtcg
     2041 tcggggtact ccgattgaca ggtcgcacac cgcttcatcg ggaccagcgt atcaggcggc
     2101 gcgggtgact tcgacggcga agcgcttctt gccgtcgatg ctgagcagcg agatcgcgag
     2161 agcgtcggcg gagacgtcga tcgtgccgaa cgattggagg ccgtcccacg gcgcgaggtt
     2221 gccggtaccg ggtggcggcg cccattggaa gacgagctcg ggaccgagcg atggatcgag
     2281 ctcgttcggc ccgaatgtgc ccgcgtggat cggaccggcg acgagctccc agaatggatc
     2341 gaagctgacg gcgctcggga ccgacgcgcg cgacggatcg aagcgatggg ctgcggcgta
     2401 gtgaacgtcg gcggtgatga agacgaagtt tttcgcgttg cgcgctttga gctccgcgag
     2461 cattgcggcc agctcgatct cgcggccgag cggcgggccg gcaacgttgc cgtaaccttc
     2521 gaagcggtcg tcgcgcgggc cgtcgggaat gatcaggccg agcggctgat cgcacgcgat
     2581 gatcttccac gtcgcgcgtg agcgcgagac ggcatcgacg aaccacgcgg cttgcgccgc
     2641 gccgagcatt gcggtgccag cgcccgcgtc attcggcgtg cggaacgagc gacagtcgag
     2701 cacgatcacg tcgagcagcg ggccgtagga gacgactcga tggacgggac cttgaggcat
     2761 cggcgtccat tcgcgcatgg cgcgttgtgc gaacgcggcg aggcgggacg cgtcgcgcag
     2821 cttcgtgtag cgatcgtcgt cgagagtctg ggagggaaac cagttgttgt gcgtctcgtg
     2881 atcgtcccac tgcgcgagga tggggacctc agccgcgagg gcgcggacgt ggtcgtcttc
     2941 gaggttgtag gcaaagcgcg cgcggaagtc gtcgaggtcc tgcgccacgc gtgcgacgcg
     3001 ctcgttcgag atgttgcgcc atagcttgcc gtcgagcgtc cgcttttcgg actcgatcgg
     3061 attgtccgcg tagatgagat cgcccgagtg gatcaggaag gctggcttgg cgtcgcgaac
     3121 tgcggcgtag ccgcgcaggc ctccccagtc gggattgcga ccgaagcctt ggccgcaggt
     3181 gtcgcccgtc cacgcgacac ggaacggagc cgtgcctggc gtcgcgaagc tcgcggttgc
     3241 ccagtcactc gtgccgcggt cggcttcgcg agtgaaccgc gcgcgcacga acacgcgctg
     3301 cgcggatggc agcttgtcga gcggtaccgt cgcggcgaaa tcggaatcgg gcgtgacggc
     3361 ggttggcgtc gcggtccggc gagcgttggc aaagcgcggc gtcgtatccc attcgagctc
     3421 gaggcgcgcg ggctcggagc agcgggccca cacgagcgcg cgctctgtcg agacgtcgcc
     3481 gatctggagg ccgtgtgtgt gctgcgggcg agcgatgcgc gtgacgggcg caacacgaca
     3541 cgcaacgagc gaagcggcgc cgccgatcat gaagccacgt cgagtgatca cgcgcatcga
     3601 gcttacctcg cgcggtgtac aatcgtcctc gtgagctcac gaccgcttgc gatcgcgctg
     3661 tgcatcttgg gcgcgtgctc gcacggcgat ccgccggaca tctcgggctt gagcgatcaa
     3721 gtcgcggtgg tggggcagca gttcgtgctc gagctgcagg gcaccgatcc cgacggcgac
     3781 gacttggagt atggagtccg ctcggatctc gagctcgaga gccgcgcgac gatttcgaag
     3841 accccgacgg gcaacggcgt gtttcgatgg acgccactcg ccgccgatgt cgggctgcac
     3901 acgttcgact tcaaggtgtc ggacggttcg aacgacacca cggtgacgat ccagatcgac
     3961 gtccgcgcga cctctgcggg cgtgcccgtg tttcgcgagc cgctgagctc ggggcgcgtc
     4021 gtcaacttgt cggcggagcc atgcatcaac gtgccgatcg tcatcgaaga tcaagacagc
     4081 gcgcaggtga cgatcgacga agaggaaccg aagatcgtcg gcgcgacgct cacgcaaagc
     4141 gatggcctca ccggcaactg gaagtggtgc ccgacgccgg cgcaggtcgc cgactcggat
     4201 cgctacacgc tcgtgctctc cgccgacgac ggcgacaatc ccaaggtgct caagccgtac
     4261 gtcatcgtgc tcggtggcgg cggtgggccc aagctcgtca tcaacgaggt cgactacgac
     4321 aacgtcggca ccgacaacgc ggagtacatc gagatctaca acggctcgtc gacgaccacg
     4381 tcgctcgcgg gtctgtctgt catcctcgtg aacggcgcga cgggcgatga gtacgctacg
     4441 atcgagctcg gtcccgtcgg ctcactcacg gcggggcagt atctcgtcat cggtggccca
     4501 gcggtcaccg ttccgaccgc ggcgaagaag ctcgacccgt tgtggacgca ggaccaggtg
     4561 cagaacggtg ctcccgatgg cgtcgcgctc gtcgataccg tcggccccac cctgctcgac
     4621 gcgctgtcgt acgaaggcgc agtcacggcg gcgaatctga ctggcttcgc gtcatctgta
     4681 tcgctcgtcg aaggcaccgc gctcgcgacc ggttccgcgg actcgaacac cgcgacggcc
     4741 acgctgtgtc gcaagccgaa cggcgccgac acgaacaacg cgtctgcgga ctggaaggtc
     4801 tgcacgacgc gcaccgtcgg caccgcgaac aattagctcg cgtgaacgct cagcagctcg
     4861 gcgagcgtgt gcacgtcgag tggcttcgca atatgcgcgg tgacaccgtc gaccgattcg
     4921 tctcgcgcgc cattggagcc gagcgcaacg acgcgcgctg ctttgaacgc agggacagca
     4981 cgcagctgct ttgcgaggtc gtatccaccg accgcgagat cgaggaacac gatctccgca
     5041 ctgaagcgtt caccggcggc gagcgcgtcg ggcgcgtcat gaacgacgac gacttcgtgc
     5101 cccatgccct cgatgacgcg tgcaaacgcg tgcgccccat cgacttcgtg atcgacgacg
     5161 agcacgcgca acggccggac cttccccagc gcaggcatct tcaccgtccg cgtcgggcca
     5221 acagcacgcg gccacgagac cgagaacgtg ctgcccttgt cctcgccttc gctcaccacc
     5281 gagacgcggc cgccgtggag ctcgacgagc tgcttgacga gcgtcaggcc gatgccgaga
     5341 ccgcccggtt tgcgatcgag cgaacgcggt ccctgcacga atgcatcgaa gatcgtcggg
     5401 acgagagtgg ccgcgatgcc gacgccgtta tcgcggacat cgatcacgat gtcgtcgaca
     5461 tcatcgcgtg cggtgacgtg gatgcgaccg cccgcgggcg tgtacgtcac cgcgttcgca
     5521 aggaggttta cgaacacctg ggtgagccgc tcgggatccg cgttgacggc caagtctgct
     5581 gccacgtcaa ccgagacggt gtggccgcgt gcgtcgattt cgctctgcac gctctcgagc
     5641 gcctgctcga tcgaccgctt cacgtcgcag gaggttcgct cgaggtcgac cttaccgagc
     5701 gtcacgcgcg acacgtcgag cagatcactg accatgtgca cgagcgacgc gagatgacga
     5761 tcgaggatcg tgagctcgcg cgagagcgtc gcttcgcgca gcttgatgag atcgaccgcc
     5821 gcggacatcg ccgcaagcgg gttgcgcagc tcgtgaccga gcatcgcgag gaactcgtcc
     5881 ttgcggcggt tcgcctcttc agccacgcgg cgcgcctccg cctccgcttc gagcagacgc
     5941 gcacgctcga gcgcttgctc gcacgcgcga gtgagcgaga acaagagctc gcgttcgtca
     6001 tcggagtaat cgtgaaaccg gccggagaac gtgaacgcga tcgcgccgag ggctcgctct
     6061 tcggttgcta gcggcaacac ggcgaacggt tgaatgcgac ccgcgttgtg cgcctcgggg
     6121 tacatctccg gcgcaagctg cgcgagctct tgctcgttgc cgacaaacat cgcggttcgc
     6181 gtcgtcgtca cgcggacgac cggaagtcgc gaatcgggcc ggatcacccg aaacggggcg
     6241 agatagtctt cggccacgtt gtgactcgcg gcgactttga agagcccgtc gctcgggtca
     6301 tggagccaga tggttgcggc gatcgcgtcc accgcgtcac ggcctgtctc gacggcgacc
     6361 ctcgcgacat ctttggacgt tcgtgcgccc gccagagctt ccaacgctcc acgcagcttc
     6421 tcgaaccgct gacgcgggtc tcccattcga ttccatgcct cacagcacga accgagccga
     6481 cgtttcgcct cttcggaaaa atcgatcgcc accgtaaggc gcaaccaata cagggcgttg
     6541 gccggggcga tttccaacgt cacggtactg ggtgcgcgcc gacgagacac tttgtaacgt
     6601 gctctgtcgc ccaccgagcg cgagcgctac aaattcaggg agttagagca tcgcgacttc
     6661 gaggacagac cgtcccagaa tcacgaggcc gcggtccgga acctcgagcc aatcctcgtc
     6721 ggtgagccgc tcgctcgcga ccatcgcaac acccgtccgc gacagcgtga acagggtcct
     6781 gccctgccgg tacgcgtaga cccgcgagtc ggaggccagc aagaagttca cggacccgat
     6841 ctcgccgagt gaatgcagag cgcggatcgc acgaacgacg cctcgctcga cgtcgcccgc
     6901 cgcatcgatg tgcgtcagca cgaatgcgaa tagtcgctcg ctgtcggtgt cgccttcgat
     6961 ctcggcgagc cgcgccagcg cgatgtgatc gctcagcgcc gcgagtgacg cgaccgtgcc
     7021 gttatgcgcg aacacgaggt tgccgcggcg aaacggatgc gtgttcgcga tcgcgaggtc
     7081 gccgacggtc ttcttgcgta catgcgcgac gacgacgcgc gccgcggtat ccgcgattgc
     7141 cgaaaagctc gtgcagtgcg ccgcgcgctc ggtgctcttg tggatctgcc agtcgccgct
     7201 cgccagcgcg attccccagc catcgccgtg ctcgtgcgag agcgcgcgca agctgcgggg
     7261 tgcatcgtgc aggagctcgc gcggcgagac ggcgtgcgtc gcgaccattc cgaacatccg
     7321 gcacatgact ctcactctat ggaacgcggg cgaaagcgcg aatattcagc gaggaattca
     7381 cgcgatcggc cacggcttat ccctggcgcc ggcctgccaa ttatcatgac ggccgtcgaa
     7441 gtgaataagt gggacctcgt gtacctcgac gccttcgagc gcgttgacgt tcaccgacac
     7501 gaagtcgccg ccgagcacgt cgagatgccc cttgccgaag cactgcaccc cgcagtgttt
     7561 gcagaagtgg cgcgtcgaga tcttgccgcc ccatacgtag ccgctcaggt cgccctcgcc
     7621 tgacagcagg cgaaacgcgt cgggcttcat gatcacgccc gtccacatca gcttcgtgca
     7681 gatcgtgcaa ttgcagcgcg acgcggtctt cagctcgaac gtggcttcga aggtgaccgc
     7741 tccgcagtgg cagcttccat gatgtttggt catggagaga gcttgccctg cggcggtgac
     7801 agccttttgt caggtttgtt tggcagccac ttcgatcgcc gcgaggacct tgccgagccc
     7861 gaccggtttg acgaagtgcg cttcgaatcc ggcctgctgc gtgcgctgct tgtcctgctc
     7921 ctgaccgtac ccggtcacgg ccatcagccg gaggccgccg ccgagtcgag tgcggagtgc
     7981 gctcgcgagc tcgtagccgt ccatgacggg gagcccaatg tcgaggatcc cgatttccgg
     8041 cgtgaaccgc tcgagcactt cgagtgcgcg cggcccatcg agcgcgacga cgacctcgtg
     8101 tccgagcgac gacagcatct cgccgagcag cgagcctgca tcttcgttgt catcgacgac
     8161 gaggatcttg cgcggcgtaa cctggacgcc ttggcgcacg ctgcgctcga ggttgggctt
     8221 ggcctgcatg tcgacgacgg gcagctcgac gatgaactcg ctgccgcgat tcacgccatc
     8281 actgatgacc ttgacggttc cgccatgcag cgtcacgagg ttcttcacga tcgcgaggcc
     8341 gataccaagg ccgccctgac tgcgatgcgt gcgccgctcg ccttggacga acaagtcgaa
     8401 caccttcggc agcagctcgg gggtgatacc gacgccgttg tccttcaccg agatgcgaac
     8461 gtgctgaccg ttgcgcttcg cgcggatgtc gatgcgcccg ccacgcggcg tgtacttcgc
     8521 ggcattcgtg acgaggttcg tgaacacctg gcacaggcgc gtctcatccg cttcgagcca
     8581 gatcgtctcg actggaatgt cgacggtaac gtggtgtcga ttctcttcgc acagcgggct
     8641 agcaatctcg accgctttcg tcacgagcga cgccagcatg tgcgggtgct tgtcgagctg
     8701 caccttgcca cgcgtgatgc gcgagatgtc gagcagatca tcgacgaggt ggatgaggtg
     8761 cgtgacttgg cgttcgatca cgctctgctc gcggaccgat gtttgatcgc cgcgaacgcg
     8821 catgagttgc accgccgtca ggatcggaga cagcgggttg cgcagctcgt gacccagcat
     8881 cgcgaggaac tcgtccttcg cgcgactggc ttgctcggct tcacggcgag ctgcctgcgt
     8941 ggcctcatgc aaacgcgcgc gctcgagtgc ttgcccgcac tgccggccga cggccagcaa
     9001 cagcgtctct tcctcggggg tgagcgagcg ctcgtgcata cagcgaaagc cgatcgcacc
     9061 gtgcgtgcgg ccttcgaaga cgatcgggat cgcgccccat gtcagcgtgc cgagtgacgc
     9121 acgcgcttcg cgcagctcgg ggaaccgagc atcgatggtg gtggcaccga caatccatac
     9181 aagcttgccg ctgcgcgcag cctccgccgc tggacgatgc gagtcgagga gctggtcttg
     9241 tagctccgac atatggctat cgtcgtaatc gccctcgacg aggatgcgtt ccgtctttgt
     9301 ccccgagcgg tcgagcatga ccacgcaagc ggccgacgcg tcgagcatcg cgcgcgtttc
     9361 atgcacgaca acgcgacatg cctcttcggc gctgacggcg cgtgacagcg ccgaggtcac
     9421 gccttggagc aacgtgacgt tgcgctctgc acgctcggcg ttcgcgcggg cgctccgctc
     9481 cgcctcgtac acacgtgcgt tcgacagtgc accggcggcc tgcgaagcga tcgcggtcag
     9541 caagttctct tcgcgctcca tgaacatcgc cggctgcgag tggccgagcg cgagcgcacc
     9601 gagcaattcg cccgacggtg cgacgagcgg cacctcgaga tagctcggca tcgcagcgag
     9661 ctcgtcgttg cgagaaccct cgaggaagca gactttctcg gcgcccgtca gccgcttcac
     9721 ttcggagatc agcttggcga gcagtcgatc gagatcgagc tcggcgccga gcatcttcgt
     9781 cacgcggaag atcgcgtcgc gatccctctc ggcgtgcttt tgcgcagtga cgtcgcggca
     9841 gaaacaccgc gtcgtgccgc ggtcgttgtc ctgctgcatg ttcgagctga tctggaccca
     9901 acgaatcgtg ccgtccttcg cacggagcgg cgcttcgtag tcctgcagca cctcgcccgc
     9961 caggagacgg cgaaggatgt ccttgatctt gtcttcgtcg gcgtgaaatt ccgagatgtg
    10021 gtgcccgacg tactcgtggc gcgcgaggcc gagcagatcc agctcggcgt tattcgccca
    10081 gcggatgacg cctttgcgat cgacgcgatg gatcggaatc ggcgcattct cgaggatgtc
    10141 ttcgaggtct tccttcgcgc ggcgcgtttc ctcttcactg cggcggacgt tattgatcga
    10201 ttcgcgcaaa gtgatctcca gctccgtacg ctttgcgatc tcgtgaacga gggactgcac
    10261 gttcgcgctg agcgccgtcg aaactgcccc gacgttctcg ggcggcagaa catgcgagtg
    10321 cgttgcgcag atcgcaggca cgccgttctg cttgtagaac gagtccatca cgtacgcgca
    10381 cagcagcgcg aagtcgtagc gaatgcgtag ctcgttccag agctcctcga gctcgagcgc
    10441 tgccgtgcga aggccgctgc gccagaggat gtccaccatc tcgccgaacg cgcgaacctg
    10501 gcgcgcactt gcgagccggc agagcccgtc gagcacgggg ccgactgtcg cgaaaaactt
    10561 ctcccggatc ggcgccgctt cgcccatgaa agcgccaagg gtctcggcgg cgtcgagcat
    10621 gacgagtcgg cccgcgtcga tctcgcgttg cacgttgatg ccgcgcgccg tcatctgatc
    10681 gacgaacatc tcgcggtggg gcgcggtcgc gatgatcacg cccggctcgc cggccttcat
    10741 tccctcggca aaaaacgccg agaccgcgtc cccgagaaag ttatcggttt cgtagaattg
    10801 gacccagtgg ggtcgcggtg tatggggcgg gaggccgaca ccatggctca cgtcacgtac
    10861 ggcctcggtc atagggaagc ctgtctctgc agaatccatg cgtgcccatg aatgctaact
    10921 acgtggtatc cgaccactcc ctgtgcgccg tcgccacacc gcgcgcatcg ttcacagctg
    10981 ttctagcagc cgtcgaacac gtagccgggc tggctcggcg tgacgaggtt caagtcccgc
    11041 aggacgatcg acagctgcgg ataggcggca caaccggtgt tgaagtcctg ctggcgatac
    11101 tcgatgacga gcacgtggtc gccgtacccg gcccgaaacg cgtcgcactc cgcgtagcga
    11161 ttgcactcct cggagacgac gaagtcggtg cccatctccg ccttgcgcga cacgatctcc
    11221 gatgagttct tctgcgcgat cgcgagcgac gcattgtgtg cagcttgcgc aaacagcttc
    11281 atcgcggcga ccgcgttgtc ttcggtgagg cgcccgccgg atcgcgagta gctatcgaga
    11341 ttgtcgatct cgaccgcatt gaagccagcc gtcgcgcatc ccgcgatcca ctcgccgacg
    11401 atcgccgcaa ccgcctgctg ctttgccggc gtgctgacgt cgatcagcat ttcattccag
    11461 tcttgatcga tgacgggatt gccgttgcca tcgcgcagga tgaggtcggc gtgcatgccg
    11521 gtccagaagc tttcctccgc ttgctggatc tggaatccat tcacgtagca gatgttgtag
    11581 accccgctcg ccggcgccgc ttcgcgatca cgcgacacga tcgtgacgcc cgcaggcggc
    11641 gtgtaggcac cgccgagctg gtagtcgagt gcgccattga gcggcggcaa tgtaaacgtc
    11701 gcgctgccgc tgccatcacc gccgcccgtc ggcgcgtcat cgagcgccat cgagtcgtcg
    11761 ccaccggagc cacgacacgc acacagcgca agcgcgagca cgagcacgag gcagcgcgtc
    11821 atgcagcaag cgtatcaccg acgaaatagc gggcgctgct cgaccgttcg agtccagacg
    11881 cgctcgcgtc gaagaggctc aaatgctgcg tgcgaccaca ctcttgctct gcatcgtagg
    11941 ctccgtcacg gttgttcgcg ccgcaccgga tccagcgccg atcgaccgaa cggagctccg
    12001 tcgtgcgctc gccgaggagc gccaggtcaa cctcgctcgc tttcacgact accgcaaaaa
    12061 gcgcgtctat ccgcacaaca cgtacgagcc cgggatgctg aacgtgtgga aggacaatga
    12121 cgatcacctc tgcgcggtcg caacgctcgt gcatctcgat ggccacgacg acatcgtcga
    12181 caccgtcgcg acgcaagaga acttcgtcaa gacggccgag ctcaccgacg gcccgctgat
    12241 cgattggatg ctgacgtcgg ggctcacgca cgaagagatc gtgatgatcc aacagccgag
    12301 cgcggccgac atcgcggcga tggatgccga gtaccgcgcc gagcagcgtc gcatcaagcg
    12361 ttggctggct cgcgaagacg cgcgcctcgc gaaggtctac aagactgtcg agaagacgct
    12421 caccgcaccg cggatcgccg atgccggtct cgatctcgcg accgcgcggc tcgcgcagca
    12481 tcccgagctc gcaaaggcgc tgctcgcaaa gcatcccgcg cattgaacga gctatcgtcg
    12541 gtgcgtgccg tcgctgtgtc ccaagtgctc cggcgagccg gtcgctgtcg acacgatgac
    12601 cgcgcgcatc gagggaacgg tcgcgcactt cgatgtcacg ctggtctgcg gctgcggcgt
    12661 gcgctggtcc gagagcaacc tgagccacgc gcaagcgctc gacaacgtcg agacggca
//
LOCUS       DATLBZ010000641         9836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6445973, whole genome shotgun sequence.
ACCESSION   DATLBZ010000641 DATLBZ010000000
VERSION     DATLBZ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9836
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6445973"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1706)
                     /locus_tag="VMZ53_31575"
     CDS             complement(261..1706)
                     /locus_tag="VMZ53_31575"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HUS33099.1"
                     /translation="MEHNTELFRGVDRWVSLSTVAYLIAAVLFILALRGLSSQETARR
                     GNMYGIVGMAIAIGATLAYRDAHFHPALIAAIVIAIVIGATMALRVGMTAMPEMVALL
                     HSFVGLAAVLVGFSLQLHLDHLRADFPAAEMPMAEKIEVYIDVIIGAITTTGSVLAFL
                     KLRGSVSGKPLLLPGRHFLNLAMLIAIVALGVMYGQHADFWKLAAAAAIACVLGVHLV
                     AAIGGADMPVVVSMLNSYSGWTASAAGFMLKNDLLIITGALVGSSGAILSYIMCRAMN
                     RSIWNVIFGGFGATPAPSTKKDGAAAKPAGDVQEIDAAGLAAKLKESKEVIIVPGFGM
                     AAARAQHAVREMTDVLRKMDVNVRFAIHPVAGRLPGHMNVLLAEANVPYDIVLEMDEI
                     NHDFPHTDVTIVIGANDIVNPAAEEDPTSPIAGMPVLQVWNSKLVIVNKRSRGAGYAG
                     IDNPLFYKPATKMLFGDSKDAIEKVVAALKS"
     gene            complement(1709..3337)
                     /locus_tag="VMZ53_31580"
     CDS             complement(1709..3337)
                     /locus_tag="VMZ53_31580"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HUS33100.1"
                     /translation="MKLGIVKETRPEERRVAASPNVVGKWTKAGWQVVVEKGAGDPAS
                     YPDSQYEAAGATMVSRADAWSADIVVKVRPPESNEVELLRDGGTLISFFYPAENEALL
                     SKLQTKQATLLAMDLVPRISRAQKMDALSSMQNIAGYRAVIEAANRFGSFFTGQFTAA
                     GKVPPGKVLIIGAGVAGLAALGAAKGLGAIVRAFDVRAAAEEQVKSLGGEFLTVTVKE
                     SGEGQGGYAKEMSKEFLEAEYALFRAQAKEVDVVITTALIPGKPAPKLWFKDMVELMK
                     PGSVVVDLAAERGGNCEYTKPGEVNEVNGVQIIGYTDLPSRMAHVASEFYGMNLWHLL
                     DEMGGHSGYKIDHENDAVRPALVLEAGQKRWPPPPMKAAAPAPAPKPAPAPAPAPKPA
                     ASAPSPRVSQTSVRTSGHHGPPARAPSKASGVVLSVLGLVAAAIWIWLRFASPDTLGS
                     AETTELIQRFTVFVMAVFIGFQVVWNVTPALHTPLMSVTNAISGIIVVGGLLSGRGDL
                     TTHVVVAVVATMFATINIAGGFIVTRRMLAMFRK"
     gene            3368..4375
                     /locus_tag="VMZ53_31585"
     CDS             3368..4375
                     /locus_tag="VMZ53_31585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33101.1"
                     /translation="MSGRIEVAGLTIDRAIVPHPLLGEAHTISQGDRVLTHMTPLDWD
                     RPTQIPAIAAPGALPPGTGGAILNLIAERALAAGVPALRYAGPYPTPALYRALLRSFR
                     ASADEATFTRDLVERASTLARDEIAIDFAPAPHRRVPHAHGMSEVRDGLERTTIDGVV
                     YERDTTAPARLVRGTPRVEFANSGVPGDAYQVPTMAAQVWFGDRLFAHVAVLDEDGTL
                     VEGPEPIPPMTSSVIGARFPPPLVAALAELLVDYVPLSSDEIAKLFATVPIVWADLGA
                     RAASRRREGGIAIHAAIWEHIAPLGLPRVAAAIAEALAPMFTSSLVEMLQALPSLRPT
                     P"
     gene            complement(4317..5069)
                     /gene="aat"
                     /locus_tag="VMZ53_31590"
     CDS             complement(4317..5069)
                     /gene="aat"
                     /locus_tag="VMZ53_31590"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HUS33102.1"
                     /translation="MPVFRLDDRLVFPPVHLAEDGLLALGGDLRPERLLLGYTQGIFP
                     WYAENLPILWHSPDPRMVMTTRDLVVQRSLKKAIRKRPYEVRTDTAFEEVLRGCAESP
                     RPGQSGTWLIPEMIDAYMKLHDLGFAHSFEAWRGDELVGGLYGVSLGAAFFGESMFAR
                     ATDASKIAFVASVRQLDAWNIGLIDCQVHTEHLERFGAYEVPRLEYLQLLTRALDEPT
                     RRGKWTLDLDLAAFTESGGEMGAPEASPRDST"
     gene            5118..8030
                     /locus_tag="VMZ53_31595"
     CDS             5118..8030
                     /locus_tag="VMZ53_31595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33103.1"
                     /translation="MRWLALAVVCISACAPVAPRFPADVQAAVAHADMRKLETDLFIV
                     YYKSNRRQEVDRFLAHADRCARQLRTEAFIKVDRKFVIVMPDAPFNNAFVAPGALGYE
                     EVSVIPTYSTLDFATQFGLPPDPGYIACHELVHYVHVQQNAGFWGTWNEWFGPTYTPQ
                     AGYDPWFLEGLATHYEARLSPGSGRPAWPLFRALFAAAYAGKHIDSGELSSFGRLASV
                     GHHYLVGSMFVDFLAEKYGERPLWATIASQSKAFTGWFYPGTFKSGFGVGFSELLDQF
                     AKWVNERFPVRAKPSTMRTLATLGNDARYARGRDGTEAWIALDVDAPARLTIRDPQGH
                     VLVEDDLVEIFPGRTLVQAEPLLISGLSITADGREVWVTVIDQGATYMVPRTLRWRRG
                     EGGLSEVRSDLGPGASIDPTGSTYYYEYVDGDRWSLAAFDTKTGAARTVIDMQPGTYV
                     LGAQVSPDGKRLAANVWNGTAFVIWVIDAATGAVTQRIGGRGLPVYDASFTSDGRLMW
                     LGEVDRRFQVIVDGYQITDAPYSALAAREANGTIRFLDREGWNWELAEVATPPPVDAS
                     SPTPPSLASQASEQPPSGPSTAVAPPTVQSDTAYSGFDHLFYPAVRAPTITVGGGLPH
                     FGLVLGGGDRLGMQRWSIAGYAQPKASTITDRTHYGASFGYANAMLAPINIIAQGGFL
                     DWAAPVATTDPMVTLTEERKTRDASLILERTWRQTFTVDVGGIYTDDRYQLETDPNIR
                     RRVAGPSAALSWYSADTTRYTGPQRALLLSAQGGFYPHQLSTFMGDITDVGGTLGIVA
                     PLPIGRRHTISATIRGRALIAKDETGLLQVGGDSALGALWNRSSVSMTSPEFDTSRFP
                     PGFRFVEPLRGYEDYAITTDQVEIGELAWKYPLIIDRGTAHFLFLPAFYLRQLDLELF
                     AAGAIDKSATKHYDVGGALTLHLQFLRIPLIVQYQLARRLVDDKALTQFVGLGADI"
     gene            complement(8035..9198)
                     /locus_tag="VMZ53_31600"
     CDS             complement(8035..9198)
                     /locus_tag="VMZ53_31600"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HUS33104.1"
                     /translation="MTVSAGCGLPDDDVEYSEVESYATVSTYVASSCSTATVIGLSKQ
                     IADEISCANPTGLTKFTAGGNLQITSNAVLPYLSAKGKQDLVDVAATRVVQVNSAFRT
                     VAQQYLLYQWYRQGRCGISIAATPGRSNHESGRAVDLANYSAVISSMSNQGWAHDVPG
                     DAVHFDHLSSPDIRGRDILAFQKLWNRNHPTDKIAEDGDYGPTTESRLRQAPSEGFPI
                     GPTCTTGTRNADIVSVNGPDRAPPQTRIHYGITIKNAGTASWPATTKLRLKMGTSSPL
                     YDSSWTSQTVITTLGAPIAAGKSGTVDFDVTTPIADAEEVPVFQELTLDDNGTKFGDI
                     EIALTVVPGMTGDESSDGNEEGDLEGGCNAGGSGTGSVLALLFVAAAVRRRRR"
     gene            9316..>9836
                     /locus_tag="VMZ53_31605"
     CDS             9316..>9836
                     /locus_tag="VMZ53_31605"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUS33105.1"
                     /translation="MKLLFCGSGWLPIVDHIAARLGDAHSITRWDLVTPLAQVVREAD
                     VLLPSNAHVTPEVITSATRLRLIQQPAAGTEKIDRAAAQARGIPICNAPGTNHVSVAE
                     HALFLLLALARRAPLAARAFAAREVGTPLGIELAGKTLGIIGMGRSGTALAERATALG
                     MHVRPLHRGANAG"
BASE COUNT         1644 a         3283 c         3230 g         1679 t
ORIGIN      
        1 ccacgtgggc acatgcgaca cgcgacgatc gacgaagggc gaacgacgaa cgacgcagcc
       61 cgcgcccacg tggacacatc gcgacacgcg acgatcgacg agctacgacc gacgaacgac
      121 gagcgacgac cgacgaccga cgaacgacga atcacgcagc ccgcgcccgc gtgggcacat
      181 cgcgacagcc gacgacgacg aacgccgacc aacccacgcc aggcacctat cgacctcggc
      241 gcaactcgca gccgcgcgtc tcagctcttg agcgcggcca cgaccttttc gatcgcgtct
      301 ttggaatcgc cgaacagcat cttcgtcgcg ggcttgtaga acagcgggtt gtcgatgcct
      361 gcgtagccgg caccgcgcga gcgcttgttc acgatcacca gcttcgagtt ccagacttgg
      421 aggacgggca tgccggcaat cggcgacgtc ggatcttcct cggcggccgg attcacgatg
      481 tcgttcgcgc cgatcacgat cgtgacgtcg gtgtgcggga agtcgtggtt gatctcgtcc
      541 atctcgagga cgatgtcgta cggcacgttc gcttcggcga gcagcacgtt catgtgccca
      601 gggaggcggc cggcgacggg atggatcgcg aagcggacgt tgacatccat cttgcgcagg
      661 acgtccgtca tctcgcgtac cgcgtgctgc gcgcgcgcgg cggccatgcc gaagcccggc
      721 acgatgatga cttccttcga ctccttgagc ttggcggcga ggccggctgc atcgatctct
      781 tgcacgtcgc ccgcgggctt ggcggcggcg ccatcctttt tcgtcgacgg agcgggggtt
      841 gcaccgaagc cgccgaagat aacgttccag atcgaacgat tcatcgcgcg gcacatgatg
      901 tagctgagga tcgcgcccga cgagccgacc agcgcgcccg tgatgatgag gaggtcgttc
      961 ttcagcatga agccggcggc cgatgcggtc cacccggagt agctgttgag catcgacacg
     1021 acgaccggca tgtcggcacc gccgatcgcc gcgacgaggt ggacaccgag cacgcatgcg
     1081 atcgcggcgg ccgctgcgag cttccagaag tcggcatgct ggccgtacat cacgccgagc
     1141 gcgacgatcg cgatcagcat tgcgaggttg aggaaatgcc ggccggggag gagcagcggc
     1201 ttgcccgaga cggagccgcg cagcttcagg aatgcgagca ccgagcccgt cgtcgtgatc
     1261 gcgccgatga tgacgtcgat gtagacctcg atcttttcgg ccatcggcat ttcggccgcg
     1321 gggaagtcgg cgcgcagatg atcgaggtgg agctgtagcg agaatccgac gagcacggcc
     1381 gcgaggccca cgaagctgtg gagcagcgcg accatctcgg gcatggcggt catcccgacg
     1441 cgcagcgcca tcgtcgcgcc gatcacgatc gcgatcacga tcgcggcgat caacgcgggg
     1501 tggaagtggg cgtcgcggta ggcgagcgtc gcgccgatgg cgatggccat gccgacgatg
     1561 ccgtacatgt tgccgcggcg tgcggtctct tggctcgaga ggccgcgcag cgcgaggatg
     1621 aagaggaccg cggcgatcag atacgcgacg gtcgacagcg acacccagcg gtcgacacct
     1681 cggaagagct ctgtgttgtg ctccacggtt acttcctgaa catcgcgagc atgcgccgcg
     1741 tgacgatgaa gccgccggcg atattaatgg tggcgaacat cgtcgcgacg acagcgacga
     1801 cgacatgggt ggtgagatcg ccgcggccgg ataacagacc accgacgacg atgatgccgc
     1861 tgatcgcgtt cgtcacactc atgagcggtg tgtggagggc tggcgtcacg ttccagacga
     1921 cctggaagcc gatgaacacc gccatcacga acacggtgaa gcgctgaatc agctcggtcg
     1981 tctcggcgga gcccagcgtg tcgggactcg caaagcgcag ccagatccaa atggcggcgg
     2041 cgaccaagcc caataccgag agcacgacgc ccgacgcttt cgacggcgct cgcgcaggcg
     2101 gaccgtgatg tcccgacgtt cgaacggacg tctgcgagac gcgcggcgac ggtgcagagg
     2161 ccgcgggctt tggcgcaggg gcaggcgcag gtgcgggctt tggtgctggc gccggtgcgg
     2221 cagccttcat cggcggcggc ggccaccgct tctgaccggc ttcgagcacg agcgcaggac
     2281 gaaccgcgtc gttctcgtga tcgatcttgt agccactgtg gccgcccatc tcgtcgagca
     2341 ggtgccacag gttcatgccg tagaactcgg acgcgacgtg cgccatgcgg ctcggcagat
     2401 cggtgtagcc gatgatctgc acgccgttga cctcgttgac ctcgccgggc ttcgtgtact
     2461 cgcagttgcc accgcgctcg gcagcgagat cgacgacgac agagccgggc ttcatcagct
     2521 cgaccatgtc cttgaaccac agcttcggag ctggcttgcc cgggatgagc gcggtcgtga
     2581 tgacgacgtc gacctctttg gcctgcgcgc ggaacagcgc gtactcggcc tcgaggaatt
     2641 ctttcgacat ctccttcgcg tagccgccct ggccctcgcc ggattccttc acggtgacgg
     2701 tcaagaactc gccaccgagg gacttcactt gttcttcggc ggccgcgcga acgtcgaacg
     2761 cgcgaacgat cgcgccgagg cccttcgctg caccgagtgc ggccaagcca gcgacgcctg
     2821 caccgatgat gagcactttt cccggcggca ctttgccggc ggcggtgaac tggccggtga
     2881 agaagctgcc gaagcggttc gcagcctcga tgacagcgcg atagccggcg atgttctgca
     2941 tcgacgacag ggcgtccatc ttctgcgcgc gcgagatgcg cggcacgaga tccatcgcga
     3001 ggagcgtggc ttgcttcgtc tgcagcttcg agaggagtgc ttcgttctcg gctggataga
     3061 agaaggagat gagcgtgccg ccgtcgcgga gaagctcgac ctcgttggac tcgggcggac
     3121 gaaccttcac gacgatgtcg gcagaccagg catcggcgcg cgagaccatg gtcgcgccgg
     3181 cggcctcgta ctgcgaatcg ggatagctgg ccggatcacc cgcgcccttc tcgacgacaa
     3241 cctgccagcc agccttggtc cacttgccca cgacattggg cgatgcggcg acgcggcgct
     3301 cttcgggccg ggtctccttg acgatcccta acttcatgaa cgggctggaa catgccacaa
     3361 atcacggatg tcgggtcgaa tcgaggtcgc cggtctaacg atcgatcgcg ccatcgtccc
     3421 gcacccgctg ctcggcgagg cacacacgat ctcgcaaggc gaccgcgtgc tgacgcacat
     3481 gacgccgctc gattgggatc gcccgacgca gattccagcg atcgctgctc ccggtgcgct
     3541 gccacctggc acgggcggcg cgatcctcaa tctgatcgcc gagcgcgcgc tcgcggccgg
     3601 tgttccagca ctgcgctacg cggggcccta cccgacgccg gcgctgtatc gcgctctgct
     3661 ccgctcgttc cgcgcgtctg ccgacgaggc gacgttcacg cgcgacctgg tggagcgcgc
     3721 atcgacgttg gcacgcgacg agatcgcgat cgacttcgcg cctgcgccac atcgacgcgt
     3781 tccgcacgca cacggaatga gcgaggtgcg agatgggctc gagcgcacga caatcgacgg
     3841 tgtggtgtac gagcgcgaca ccacggcgcc agctcgtctc gttcgtggaa cgccccgcgt
     3901 cgagtttgcg aacagcggcg tgcccggcga cgcgtatcag gtgccgacca tggccgcgca
     3961 ggtctggttc ggcgatcggt tgttcgccca cgtggcggtg ctcgacgagg acggcacgct
     4021 ggtcgaagga ccggagccga ttccaccgat gacgtcgtcg gtgatcggcg cgcgctttcc
     4081 tccgccgctc gtcgcggcgc tcgcggagct cctcgtcgac tacgtgccct tgtcgtcgga
     4141 cgagatcgcc aagctgttcg cgacggttcc gatcgtgtgg gccgacctcg gtgcgcgcgc
     4201 agccagtcgt cgacgagaag gcggcatcgc gatccatgcg gcgatctggg agcacatcgc
     4261 gcctctcgga cttccacgcg tcgcggcagc aattgcggag gcgctggccc cgatgttcac
     4321 gtcgagtctc gtggagatgc ttcaggcgct cccatctctc cgcccgactc cgtgaatgcg
     4381 gcgagatcga ggtcgagcgt ccacttgcct cggcgtgtcg gctcgtcgag cgcgcgcgtg
     4441 agcagctgca agtactcgag ccgcggaacc tcgtacgcac cgaaacgctc gagatgctcg
     4501 gtgtggacct gacaatcgat caggccgatg ttccatgcat cgagctggcg cacgctcgcg
     4561 acgaacgcga tcttcgacgc atccgtcgcg cgcgcgaaca tcgactcgcc gaagaacgct
     4621 gcgccgagcg acacgccata gagcccgccg acgagctcgt cgccgcgcca cgcctcgaac
     4681 gagtgcgcga agccgagatc gtggagcttc atataggcgt cgatcatttc cggaatcagc
     4741 cacgtgccgc tctgcccggg ccgcgggctc tccgcgcagc cgcgcaggac ctcctcgaac
     4801 gccgtgtcgg tgcgcacctc gtacggccgc ttccggatcg ccttcttcag gctgcgctgc
     4861 acgacgagat cgcgcgtcgt catcaccatc cgcggatcgg gcgaatgcca caggatcggc
     4921 agattctcgg cataccaggg gaagatgcct tgcgtgtacc cgagcagcag acgctccggg
     4981 cgcaggtcgc cgccgagcgc gaggaggccg tcctcggcaa ggtggaccgg cggaaacacg
     5041 agtcggtcat caagccggaa aaccggcatg agggaagtgt agcggacagc cggaagctgt
     5101 ggagatagta cgggcccatg aggtggctcg cgctagcggt cgtgtgcatc agtgcctgtg
     5161 cgcccgttgc accgcggttt cccgcagatg tgcaggcagc tgtcgcacac gccgacatgc
     5221 gcaagctcga gaccgaccta ttcatcgtct attacaaatc gaatcgacgg caggaggtcg
     5281 atcgcttcct cgcgcatgca gatcgatgtg cgcgccagct ccgcaccgag gcgttcatca
     5341 aggtcgatcg caagttcgtc atcgtgatgc cggacgcgcc gttcaacaac gcgttcgtcg
     5401 cgccgggtgc gctcggctac gaagaggtct ccgtcatccc gacgtactcg acgctcgatt
     5461 tcgcgacgca gttcggcctg ccgcccgacc cgggctacat cgcgtgtcac gagctcgtcc
     5521 actacgtgca cgtgcagcag aacgccggct tctggggaac gtggaacgag tggttcggcc
     5581 cgacgtacac gccgcaagcg ggctacgacc cgtggtttct cgagggactc gcgacgcact
     5641 atgaagcgcg cctctcgccg ggatccggac gtccggcgtg gccactcttt cgcgcgctgt
     5701 tcgcggcggc gtacgcgggc aagcacatcg attcgggcga gctctcatcg ttcggacgcc
     5761 tcgcgtcggt cggccatcac tatctcgtcg gctcgatgtt cgtcgacttc ctcgccgaga
     5821 agtatggcga gcggcccttg tgggcgacga tcgcgagcca gtcgaaagcg ttcacgggtt
     5881 ggttctatcc gggcacgttc aagagtggct tcggcgtcgg cttttccgag ctgctcgatc
     5941 aattcgcgaa gtgggtgaac gaacggttcc cggtgcgcgc gaagccgtcg accatgcgca
     6001 cgctcgcgac gctcggcaac gatgcgcgtt acgcacgcgg ccgcgatggc accgaggcgt
     6061 ggatcgcact cgacgtcgat gcgccggcgc gcctgacgat tcgcgatccg cagggccacg
     6121 tcctcgtcga ggatgacctc gtcgaaatct tccccggccg cacgctcgtg caagccgagc
     6181 cactcctgat ctccggcctc tcgatcaccg ccgacggccg cgaggtctgg gtcacggtca
     6241 tcgatcaagg cgcgacctat atggtgccgc gcacgctgcg ctggcgtcgt ggcgaaggcg
     6301 gcctctcgga agtccgcagc gatctcggcc ccggtgcgtc gatcgatcca acgggctcga
     6361 cctactacta cgaatacgtc gacggcgatc ggtggagcct cgcggcgttt gacacgaaga
     6421 cgggcgcggc gcgcacggta atcgacatgc agcccggcac gtacgtgctc ggcgcgcagg
     6481 tatcgcccga cggcaaacgc ctcgccgcga acgtgtggaa cggcacggcg ttcgtcatct
     6541 gggtgatcga tgcagcgacg ggcgctgtca cgcagcgcat cggtggccgc ggccttcccg
     6601 tctacgacgc gagcttcacg agcgatggcc gcctcatgtg gctcggcgaa gtcgaccgcc
     6661 gcttccaagt gatcgtcgac ggctaccaga tcacggacgc accctactct gcgctcgcgg
     6721 cgcgcgaagc gaacggaacg atccgctttc tcgatcgcga aggctggaac tgggagctcg
     6781 ccgaggtggc aacaccgcca cctgtcgacg cgtcatcgcc gacgccacca tcactcgctt
     6841 cgcaagcgtc ggagcaaccg ccgagcggtc catccacggc ggtcgcgccg ccgaccgtgc
     6901 agtccgacac cgcgtactcc ggtttcgatc acttgttcta cccagcggtg cgcgcgccca
     6961 cgatcaccgt cggtggaggc ctgccgcact tcggcctcgt gctcggcggc ggcgaccgcc
     7021 tcggtatgca gcgctggagc atcgccggct acgcgcagcc aaaggccagc acgatcaccg
     7081 atcgcacgca ttacggcgcg agcttcggct atgcgaacgc gatgctcgcg ccgatcaaca
     7141 tcatcgcgca gggcggcttc ctcgactggg ccgcgcccgt cgcgacgacc gatccgatgg
     7201 tcacgctcac ggaagagcgc aagacgcgcg acgcgtcgct gatcctcgag cgcacgtggc
     7261 ggcagacgtt caccgtcgac gtcggcggca tctacaccga tgaccgttat cagctcgaaa
     7321 ccgaccccaa catccggcgt cgcgtggccg gaccttccgc cgcgctctcc tggtactcgg
     7381 cggacacgac tcgctacacg ggcccgcagc gtgcgctcct cctctcagcg caaggcggct
     7441 tctatccgca tcagctctcg acgttcatgg gcgacatcac agatgtcggc ggcacactag
     7501 gcatcgtcgc gccgctgccc atcgggcgtc gccacacgat cagcgccacg attcgcggtc
     7561 gcgcgctcat cgcgaaagac gagactggcc tgcttcaagt cggcggcgat tccgcgctcg
     7621 gcgcgctctg gaatcgatcg tcggtctcga tgacgtcgcc cgagttcgac acgtcgcgct
     7681 ttccaccggg cttccggttc gtcgagccac tccgaggcta cgaggactac gccatcacga
     7741 ccgaccaagt cgagatcggc gagctcgcgt ggaagtaccc gctcatcatc gatcgcggca
     7801 ccgcgcactt cctgtttctt ccggccttct acctgcggca gctcgacctc gagctcttcg
     7861 ccgcgggcgc gatcgacaag tcggccacga agcactacga cgtcggcggc gcgctaaccc
     7921 ttcatctcca gttcctccgc atcccgctaa tcgtccagta ccaactcgcg cgccgcctcg
     7981 tcgacgacaa ggcgctcacg cagttcgtcg gcctcggcgc cgacatctag cgacctagcg
     8041 acgccggcga cggaccgccg ctgcgacgaa tagcagcgcg agcacgctgc cggtgcccga
     8101 gccgccggcg ttgcagccgc cttccaggtc gccttcttcg ttgccgtcgc tgctctcgtc
     8161 gcccgtcatg cccgggacaa ccgtcagcgc gatctcgata tcgccgaact tcgtgccgtt
     8221 gtcgtcgagc gtcagctctt ggaacacggg aacttcctcg gcatcggcga tcggcgtcgt
     8281 gacgtcgaag tcgacagtgc cgctcttgcc tgcagcgatc ggtgcgccga gcgtcgtgat
     8341 gacggtctgc gacgtccacg aggaatcgta gagcgggctc gacgtgccca tcttgaggcg
     8401 gagctttgtc gtcgccggcc agctcgcggt gcctgcgttc ttgatcgtga tgccgtagtg
     8461 gatgcgcgtc tgcggcggtg cgcgatcggg tccgttgacg gagacgatgt cggcgttgcg
     8521 cgtgccggtc gtgcacgtcg gtccgatcgg gaagccttcc gatggcgctt ggcgcagccg
     8581 cgattctgtc gtcgggccgt agtcgccgtc ttctgcgatc ttgtcggtcg gatggttgcg
     8641 gttccagagc ttttggaacg cgaggatgtc gcggccgcgg atgtcgggcg acgagagatg
     8701 atcgaagtgg acggcgtcgc cgggcacgtc gtgcgcccag ccttggttcg acatcgagga
     8761 gatgaccgcg gagtagttcg cgaggtcgac ggcgcgaccg ctctcgtggt tgctgcgtcc
     8821 cggcgttgcc gcgatcgaga tgccgcagcg gccctgccga taccattggt acagcaagta
     8881 ttgctgcgcg accgtgcgaa aggcgctgtt gacctgcacg acgcgagtgg ccgcgacgtc
     8941 gacgagatcc tgcttcccct ttgccgagag gtagggcagc accgcgttgc tcgtgatctg
     9001 gaggttgccg cctgccgtga acttggtgag gcccgttgga ttcgcgcagc tgatctcgtc
     9061 ggcgatctgc ttcgagagcc cgatcaccgt cgcggtcgag cagctggacg cgacatacgt
     9121 cgacaccgtc gcgtagctct cgacctcgct gtactcgacg tcgtcgtcgg gcagaccgca
     9181 gcctgccgag acggtcatcc ccacagcgag aacgatgctg tgcaacatcg aggttgcagc
     9241 tgctttgtca tttcgctgag ttggacgtcg agagcgcatg tgcgcagggc attcagcaag
     9301 tacgatgccc aggtcgtgaa gctcttgttc tgcggttctg gctggctgcc gatcgtcgac
     9361 cacatcgcgg cacgtcttgg tgacgcgcac tcgatcacgc gctgggacct cgtcacgccg
     9421 ctcgcgcagg ttgtccgaga agcggacgtc ttgctgccgt cgaacgcgca cgtcacgccc
     9481 gaggtcatca ctagcgcgac gcgcctccgg ctcatccagc agcctgccgc gggcaccgag
     9541 aagatcgatc gcgcagctgc tcaggcgcgc ggaattccga tctgcaatgc ccccggaacg
     9601 aatcacgtct cggtggcaga gcatgcgctc tttctgctgc tcgcgctcgc gcgtcgcgcg
     9661 cctctcgcag cacgcgcatt cgcagcgcgt gaagtcggca cgccgctcgg catcgagctc
     9721 gccggcaaga cactcggcat catcggcatg ggccgctcgg gtaccgcgct cgcggaacgc
     9781 gcaaccgcgc tcgggatgca cgtgcggcct ctgcatcgcg gcgcgaatgc gggcga
//
LOCUS       DATLBZ010000642         5234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8814230, whole genome shotgun sequence.
ACCESSION   DATLBZ010000642 DATLBZ010000000
VERSION     DATLBZ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8814230"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VMZ53_31610"
     CDS             complement(<1..754)
                     /locus_tag="VMZ53_31610"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HUS33106.1"
                     /translation="MIIALVFLAYIIRPVVKELFLAAVFAGVLWPVQQWLSKRLRGKR
                     GLAAALITIGVVILLLGPIALMVTFVIRDGADGVTFVSDALHSEDVKKLVDRLPESAG
                     NAVQDAIDRVPRDVGGMVGELGGDRKGETAATVGKALSKTGSLLFHTVLMLIALFFML
                     VRGDDLVAWIDNVSPLRKGQTRELLTTFRRVSFAVIASAAVTAAVQAIAALIGYFIAR
                     VPSPWFFALVTFFLAFVPAIGAGVVCLFAAALL"
     gene            complement(876..1754)
                     /locus_tag="VMZ53_31615"
     CDS             complement(876..1754)
                     /locus_tag="VMZ53_31615"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HUS33107.1"
                     /translation="MRFAPLLLGVVVVACGRQDARSVASYEVPIEAAPTEAPAPRPEA
                     PCAADIVLGPGLEHERRAIAATKSPTSDTPCLDIVRADLATYRLRVLSSSRDGNGSHP
                     APWWRDTFHLVAVTNAGMFHEGGDPVGLVVEDGATLGHDNKKFSGYLAFDDAGNATIA
                     GRDCPGFDLAKLRTQYRSVVQSGRLLGCNGEALPWKDKKQYSAAAIGLSRDRKQVVFL
                     HARDAVTMTELATAVAELDLSGAIFLEGGPEASLVVRGAKSELSRVGSYETGFVENNE
                     NQSFWWLPNVIALETK"
     gene            complement(1900..2829)
                     /locus_tag="VMZ53_31620"
     CDS             complement(1900..2829)
                     /locus_tag="VMZ53_31620"
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HUS33108.1"
                     /translation="MFDQSVLRSVGQRVLVAGCGGGYDIMGAVPLLHALRAAGRDVHL
                     ASLSFCYLNGLTNAQQDAEVPNLYAVRADAATPRAYCPEAHLAKFLDDELGGSHTIWS
                     FDKTGVRPLARAYRALITRLQIDSIILVDGGIDAVLRGDETSLGTPSEDLATLAAATM
                     QDIPVVLACVGMTSELRDGIQHAQVFERFAELTRDGAFLGASSLVPGTPACDLYVKSV
                     EAVFAGQSEQKQSHVHRVITKSLRGEFGATAPHVWLSPLLHLYWFFDALAVARTHCFL
                     DELRATDSIWEVAARIEAARKSLPIKDRTQIPI"
     gene            complement(2883..3623)
                     /locus_tag="VMZ53_31625"
     CDS             complement(2883..3623)
                     /locus_tag="VMZ53_31625"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HUS33109.1"
                     /translation="MSVRVVVITDRRLYGSEELAAHVARILRAVPRGSVAVQVREKDL
                     DGGPLATLAREVLEVTKPAGAPLWINDRLDVAYAIGATGVHLPERGLAIDDALTACAA
                     ANMSDDSFADEESVELDVRGALSKREGLAIGCSRHTAEAVCEAAANGASLVQLGPIFA
                     TPNKGAPLGAEVLAVRTQLPDHVSLVAVGGIDSADRAREAAYAGADAVAVIRAAWTAD
                     SPHLIAELVDAVEAGIGMRMLSRTITPR"
     gene            complement(3629..4399)
                     /locus_tag="VMZ53_31630"
     CDS             complement(3629..4399)
                     /locus_tag="VMZ53_31630"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HUS33110.1"
                     /translation="MSDTWTLGNREFSSRIIVGTGKYASLDETRAALAASGAEIVTVA
                     LRRVDLKSKEPTVLDAIDRKKYVLLPNTAGCYTADEAIRTLHLARELGMDELVKLEVI
                     GDPKTLFPDNTATVEAAARLVKEGFKVLPYCSDDLITCKRLADAGCLAVMPLAAPIGS
                     GLGIRNPHNIRLILENVNVPVIVDAGVGTASDAAIAMELGCTAVLMNTGIAGAKKPVL
                     MAEAMRLAVDGGRKAFLAGRMPRRDYANASSPNAGLIE"
     gene            complement(4405..4617)
                     /gene="thiS"
                     /locus_tag="VMZ53_31635"
     CDS             complement(4405..4617)
                     /gene="thiS"
                     /locus_tag="VMZ53_31635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HUS33111.1"
                     /translation="MSENIDVVVNGELRTVTPGTTVAALITELGLADRRVAVERNREV
                     VPRAQHSSTVLAAGDRLELVTFVGGG"
     gene            4953..>5234
                     /locus_tag="VMZ53_31640"
     CDS             4953..>5234
                     /locus_tag="VMZ53_31640"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUS33112.1"
                     /translation="MQGEDPARIRATIARAALFAQLPINAIEDLTGRMTVRKVAVGSA
                     VVSQDEPGDAMFLIMNGRVKVVMFGESGREVTLSLLRAGDSFGEMSLFDQ"
BASE COUNT          936 a         1695 c         1768 g          835 t
ORIGIN      
        1 gcaggagcgc ggcggcgaag aggcagacga ccccagcgcc gatcgccgga acgaacgcga
       61 ggaagaacgt gacgagcgcg aagaaccacg ggctcggcac gcgcgcgatg aagtagccga
      121 tcagcgcggc gatcgcttgg accgcagcgg tgacggcggc ggatgcgatg accgcgaacg
      181 agacgcggcg gaacgtggtg agaagctcgc gcgtctggcc tttgcggagc ggtgagacgt
      241 tgtcgatcca cgctacgaga tcgtcgccgc gcacgagcat gaagaacagc gcgatgagca
      301 tgaggacggt gtgaaacaac aacgagcctg tcttcgacaa tgccttgccg acggtcgccg
      361 ccgtctcacc tttgcgatcg ccgccgagct cgccgaccat tccgccgacg tcacgaggca
      421 cgcgatcgat agcgtcttgc acggcgttgc cggcgctctc tggtaggcga tcgacgagct
      481 tcttgacgtc ctcgctgtgg agcgcatcag agacgaaggt cacgccgtct gcgccgtcgc
      541 ggatgacgaa ggtcaccatc agcgcgattg gaccgagcag taagatcacg acgccgatcg
      601 tgatgagagc agccgcgagg ccacgcttgc cgcgcaggcg cttcgagagc cattgctgca
      661 cgggccagag gacacccgcg aacacggcgg caaggaagag ctctttaacg acgggccgga
      721 tgatgtacgc gagaaacacc agcgcgatga tcatcaacgc gaggaagaat cgcggtaccg
      781 gccgttcgcc ggggctcacg cgagccctct gcgaacattt ccggctctca gcgactctcg
      841 cgcacgcatg atgaaaatgc gtgcatatcg cgtgctcact tcgtctcgag cgcgatgacg
      901 ttcggcaacc accagaaaga ctggttctcg ttgttctcga cgaatccggt ctcgtagcta
      961 ccaacgcggc tgagctcgga cttcgcgccc cgcacgacga gcgaggcctc ggggccaccc
     1021 tccaggaaga tcgcgcccga cagatcgagc tcggcgaccg cggtggcgag ctccgtcatc
     1081 gtcacggcgt cgcgcgcgtg gaggaacacg acctgcttgc gatcgcgcga cagaccgatc
     1141 gcggcggcgg agtactgctt cttgtctttc cagggcagcg cttcgccatt gcagccgagg
     1201 aggcgaccgc tctggacgac ggaccggtac tgcgtgcgga gcttcgcgag atcgaagcca
     1261 gggcagtcgc gacccgcgat cgtcgcgttg cctgcgtcgt cgaacgcgag atagcccgag
     1321 aacttcttgt tgtcgtggcc gagcgtcgcg ccgtcttcga cgacgaggcc gactggatcg
     1381 ccaccttcgt ggaacatgcc ggcgttcgtc accgcgacga gatggaaggt gtcgcgccac
     1441 cacggtgcgg gatgcgagcc gttgccatcg cgcgacgacg aaaggacgcg caggcgatag
     1501 gtcgcgaggt ccgcgcgcac gatgtcgagg catggcgtat cgctggtcgg cgacttggtc
     1561 gcggcgatcg cgcgccgttc gtgctcgagg cctgggccaa gcacgatgtc agctgcgcac
     1621 ggcgcctcgg gccgcggcgc gggcgcttcg gtgggagccg cttcgatcgg gacttcgtag
     1681 ctcgcgaccg acctcgcatc ctgacgacca caagccacga ccacgacgcc gagcaagagt
     1741 ggggcgaaac gcacggggcc ggaacgcgcg gcgccgccag gccattccag gaaaataggt
     1801 cggactgaat ttccgggtgc agggcgctcg gaaaataggt cggactgaat ttccgggggc
     1861 aggcgagtcg gaaattcggt cggactgaat ttccccggct cagatcggga tctgcgtgcg
     1921 gtctttgatc gggagtgact ttcgagcggc ctcgatccgc gcggcgacct cccagatcga
     1981 atccgtggcg cgaagctcgt cgagaaagca gtgggtgcgc gcgaccgcga gtgcatcgaa
     2041 gaaccagtag aggtgcagga gcggggacag ccagacgtgc ggcgcagttg cgccgaactc
     2101 gccacggagc gacttcgtga tcacgcggtg gacgtgactc tgcttctgct ccgactgccc
     2161 cgcgaacaca gcttcgacag acttcacgta gaggtcgcac gccggcgtgc ccggtacgag
     2221 tgacgatgcg ccgaggaacg cgccatcgcg cgtgagctcc gcgaagcgct cgaagacttg
     2281 ggcgtgctgg atcccgtcgc gcagctcgga cgtcatgccc acgcatgcga gcacgaccgg
     2341 gatgtcttgc atcgtcgcgg cggcgagcgt cgcgaggtct tccgacggcg tgccgagcga
     2401 cgtctcgtca ccgcgcagca ccgcgtcgat gccgccgtcg acgaggatga tcgaatcgat
     2461 ctgcaggcgc gtgatcagcg cgcggtaggc gcgggcgagt ggacgcacgc ccgtcttgtc
     2521 gaagctccag atcgtgtgcg agccgccgag ctcatcgtcg aggaacttcg cgaggtgcgc
     2581 ttcggggcag tacgcgcgcg gcgtggcggc gtcggcgcgc acggcgtaga ggttcgggac
     2641 ctcggcgtct tgctgcgcgt tggtgagccc gttcaagtaa cagaacgaaa gactcgcgag
     2701 atgaacgtcg cggcctgccg cgcgcagcgc gtggaggaga ggaaccgcgc ccatgatgtc
     2761 gtagccgccg ccgcatcccg cgacgagcac acgctgaccc acgctacgca ggaccgactg
     2821 atcgaacatc tcctcatggt gtcaggcgga caccaaggct gcaacgccta catcggcggc
     2881 tcttagcgcg gcgtgatcgt tcgactcagc atgcgcatgc cgatgcctgc ctcgacggcg
     2941 tcgaccagct cggcgatcag atgaggcgag tctgcggtcc acgcggcgcg gatcacggcc
     3001 actgcatcgg cacctgcata agcggcttcg cgcgcgcggt cggccgaatc gatcccaccg
     3061 acggcgacga gcgagacgtg atccggcagc tgcgtgcgga ccgcgaggac ctccgcgccg
     3121 agcggcgcgc ctttgttcgg cgtcgcgaaa atggggccga gctgcacgag cgacgcaccg
     3181 ttcgccgcgg cttcgcacac cgcttcggcc gtgtggcgcg agcagccgat cgcgagacct
     3241 tcgcgcttcg ataaggcgcc gcggacatcg agctcgaccg attcttcgtc ggcgaacgag
     3301 tcatcggaca tgttcgcggc ggcgcacgcg gtcagcgcat cgtcgatcgc gaggccgcgc
     3361 tccggcaggt gcacaccggt cgctccgatc gcgtacgcga cgtcgagacg gtcgttgatc
     3421 cagagcggcg cgccggcggg cttcgtcacc tcgaggactt cgcgcgcgag cgtggcgagc
     3481 gggccaccat cgagatcctt ctcgcggact tggaccgcga ccgagccgcg cggcactgcg
     3541 cgcagaattc gcgcgacatg cgcggcgagc tcctcacttc cgtacaggcg tcgatcggtg
     3601 atgacgacga cgcgcacgct catcggagct actcgatcag gccggcgttc ggcgacgacg
     3661 cattcgcgta atcgcgacgc ggcatgcggc cggccaggaa cgccttgcgt ccaccgtcga
     3721 cggcgagccg catcgcctcg gccatcagca ccggcttttt cgcgccggcg atgcccgtgt
     3781 tcatgagcac tgccgtgcag ccgagctcca tcgcgatcgc cgcatcgctc gccgtgccca
     3841 cgcccgcgtc gacgatcacg ggcacgttca cgttctccaa gatcagccgg atgttgtgcg
     3901 gattgcggat gcccaggccc gagccaatcg gcgccgcgag tggcatcacc gcgaggcacc
     3961 ctgcatcggc gagccgctta catgtgatga gatcatccga gcagtagggc aggaccttga
     4021 agccctcttt cacgagccgc gcagccgcct cgaccgttgc ggtgttgtcg ggaaacagcg
     4081 tcttcggatc gccgatcact tcgagcttca ccagctcgtc catgcctagc tcgcgcgcga
     4141 ggtgcagcgt ccgaatcgct tcgtcggccg tgtaacagcc cgccgtgttc ggcagcagga
     4201 cgtacttctt gcgatcgatc gcgtcgagga ccgtgggctc tttgctcttc agatcgacgc
     4261 ggcggagcgc gacggtgacg atctcggcgc cactcgcggc aagcgcggcg cgcgtctcgt
     4321 cgagcgaggc gtacttgccc gtaccgacga tgatgcgact cgaaaattca cggttcccga
     4381 gtgtccaggt atcagacatt tcgatcagcc tccgccgacg aacgtgacga gctcgagccg
     4441 atcgccggca gcgagcacgg tggacgaatg ttgcgcacgt ggcacgacct cgcggttgcg
     4501 ctcgacagca actcggcgat ccgcaaggcc gagctcagtg atgagcgcag cgacggtagt
     4561 acctggtgtc accgtacgaa gctctccgtt gacgacgacg tcgatgttct ccgacatcgg
     4621 ggcggacgct accacgtgga agtcaatgac gcagtgcggg gggtgtgatg accacgacat
     4681 ccgcgccccg gtcgccaacg aagttgtgaa gcatccgtca catggaaacc ccttggaata
     4741 ggaggcaagc ccccgaaacg ccatcgtcgc tgagatcggt tctgcgcggg catgacactc
     4801 gcagagagaa ccgtctcgtg ggcgctcgtt gacctcaggc ccacgaggag caccctatgt
     4861 cgctcggaat tgcgcgtccg atttctcctg tcatgcctat gcgcccgtcg gcgccggcac
     4921 ccaccaacac catgcccgcg aacgatccgc gcgtgcaggg tgaagaccca gcgcgcatcc
     4981 gcgcgacgat tgcgagggcg gcgctgtttg ctcagctgcc catcaacgca atcgaggacc
     5041 tcacgggccg catgacggtc cgcaaggttg ccgtcggctc ggcagtcgtt tcacaggacg
     5101 agcccggcga cgcgatgttc ttgatcatga acggccgcgt gaaggtcgtg atgttcggcg
     5161 agagcggccg cgaagtgacg ctgtcgctgc tccgcgctgg cgactcgttc ggcgagatgt
     5221 cgctgttcga ccaa
//
LOCUS       DATLBZ010000643        10547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8814396, whole genome shotgun sequence.
ACCESSION   DATLBZ010000643 DATLBZ010000000
VERSION     DATLBZ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8814396"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VMZ53_31645"
     CDS             <1..928
                     /locus_tag="VMZ53_31645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33113.1"
                     /translation="KKKEEVDKFIKQLADCAAKADSGGNVQPQSLDRPTMPTTTTTPT
                     TTTPTTTTTKPNTTTPTTTSTTQKPTTTTTTPTTTSSTTTPTTTSTTPTTTSTTPTTT
                     TATTTTPTTTPTTTAQLENPDEEPEEQEPSVRAQTGTGATMISARAEGGVALLGGKDL
                     KFPAQPVFAVSGGYPLAAGPAVVELGAGISFSPLPYQAMGEQKQGSMIGARVQAVGAY
                     PVAPKLWLRGELGVGVVALGGLEMGNPLTTDGSAKSFTLLSVRAGVAADYEITPNIVA
                     TLQPFAFAFSPKGDGIGALREIDVLVGLGYRK"
     gene            complement(916..2208)
                     /locus_tag="VMZ53_31650"
     CDS             complement(916..2208)
                     /locus_tag="VMZ53_31650"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HUS33114.1"
                     /translation="MKLLDKLGEPSRYLRQLERLYARVAETRRVFELQQDGVPLSDLV
                     NDKGKLAKLLARAVRDGSFRSTPAPRYFARLDKDRELVRFSLFDVLVHAVAAEILDEA
                     IATRLSPRLFSFRAGRSSWQAVRDLALFTRRHRRARPDPRTRGFYVLRADVRRFCESV
                     PLADHAPLWPLLAEVLGEDLPLMRSLLRPTVLDADEQPASPIVGLPFGSPITNVILNL
                     YLSSIDETLAAVPGGFYARFGDDLIFAHDDAAKTTWALDEVRGRIEKLGLTLSPHKVR
                     SLYFNGAGRSSTSFAGSKAIAFLGCEVTFDGTIRLPRDKWRELLLDVRARIRRTHAIS
                     RDAGEAARLDVLCRVVNEALDPLSPLAQRYAPLLAQLVTDRHQLAELDHHVALALAET
                     LSGMRGPRAFRRVSWSRLRASGLQSRIAQRNSGRAYLR"
     gene            complement(2348..4096)
                     /locus_tag="VMZ53_31655"
     CDS             complement(2348..4096)
                     /locus_tag="VMZ53_31655"
                     /inference="COORDINATES: protein motif:HMM:NF041328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6577-like cysteine-rich protein"
                     /protein_id="HUS33115.1"
                     /translation="MKHLWLAFLLGLAAIGCGNITRECPSGLTDCDGTCADMQSDNQQ
                     CGECGNTCNAMEQCVAGTCQCTSGGKLCGANCVDTATDEANCGDCGVACGTGLTCNDG
                     TCGCPSNQMDCSGTCIDTMTDSNNCGSCGHACLAGAACINGGCGCPTNETQCGTICAN
                     TQTDNSHCGNCTTVCGGGKTCQTGSCACTGSNILECNGACINKLTDNSNCGACGTTCS
                     GGKTCQTGSCQCTGGQTACGGTCYDVSADRAHCGTGCGVCSATQLCQNGCAAAPSLVI
                     TSQWESPTGWLRPTNQPIAMTFALSPASTATGITYECRTYLASGTPPAFAPCDGSTGV
                     GTTYRPAAQADGTYKTEVRYLQNGVAIGSPTVYTFYAHRDLNNAAKCPLAPTDTAYFT
                     AAANYATANPALFPIPAAPLFTADPALKIANPFINVPFKQVKASRSMLNSASTWGTFP
                     KDFVVKDLSLRHSFILGNSNKLLMMKRNYAAAGGSCQNLSQMHHVGNLTFNFAMCDVF
                     ILNINGIGLCVGRNASTNQPEVKGTPYVAGWWRMRTVRYPSRYMSLTNQTRSSAYCAT
                     PGCRAASSNTLIELPP"
     gene            complement(4093..6105)
                     /locus_tag="VMZ53_31660"
     CDS             complement(4093..6105)
                     /locus_tag="VMZ53_31660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33116.1"
                     /translation="MDDLQRFLRRRARAAERRVSLEEALLGGRFARYALFRLKWVGLA
                     RGLGLITHIVEFTVLMALFSQRHLAMSVALQNGNRLFDGLWWGALEVMRRRIRADGTK
                     AFVWQETSRWLSAAMWLGLAVVIAGVALLGWRWHARGAPGVFEAYALVCVARLAIDLV
                     SRTLYSGVWARQRVFRPMVAIMLAEPLGVLVVALGWRVLGAWAFPVGLLANILISRGL
                     TIAYTLRAYRVARLHPPRVSLRFPSRKLGEKLHKGEVAWAAAGNLTARIGSAMLLATF
                     VRAPFHMAKLDAFPYVLQLAVPLLSTATSWSRVFYHDFKRLEEEAATALCRKLERRLW
                     VVSIIVGLVIWAAVAAVAQWRVGLALVRQPVAALGPLCLALSILSVLQLRELARGGFI
                     RLFIGSVATCVAILIAIWRGAPDDATAGLMLASALSVGAVVLAVGGSRRTHTTAGVHR
                     GIESWVRALREMRGPTRVGVLQVEARGTAPRLLAERWAERLGRRGGVTLRARRLLWFE
                     EGSVDNAAERALLAAGLGRALDVTEVAPDGHQALERAIAAGLLREPQGEHADEKALMA
                     EFRQRFPQGLVASLADKDVPAGFAALPPRLRKRLWRDALRPRRRRDLPFEITAFRPAG
                     VIKVLFAVPRDSSREERIAWRARVEAANWCCAVAPNGIRSDGDSPK"
     gene            6207..7445
                     /locus_tag="VMZ53_31665"
     CDS             6207..7445
                     /locus_tag="VMZ53_31665"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HUS33117.1"
                     /translation="MARRPVRRRSFGRRRARRVRQMEAVGLPAPLHTVSTPVDGGALR
                     VLGSGDQAYQTILERVRNAEKSVEIRAFLWRDDEAGNMMADAVLQAADRGVQVTIQKD
                     RIAAVYEYTGGNKQSFFHKRVDPIRGIQAWFLGAVTRAKGSFKQKPNELALRILQHPN
                     IRVEHMRKRFDHSKLYIIDDRILALGSMGIGDNHRHEWLDVMVEAEGEEYVARLRERM
                     LGHDEFDPSRGLDFLVHSREAHRKKSCPMISHRLALIEAAQVSITVEMAYFGDRRFTA
                     VLARAVQRGVQVKVVTADRADVLANINRATCDALMKLTGAPQNLTIVLLPRMVHSKVV
                     VIDHRWCDVGSANFTSLSHGVYDEINLYVDCEPFARDLEAEIDSHCLDGRIADSRLTY
                     RKVASGVERAILAYQSRRGG"
     gene            7502..8059
                     /locus_tag="VMZ53_31670"
     CDS             7502..8059
                     /locus_tag="VMZ53_31670"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS33118.1"
                     /translation="MTAAKLVFADAGYHGASINAIIEKAQIARGTFYLYFESKAAVFD
                     AILDQAMGELRSRIHRIEVVDANAPPPQVQLREQILATLDYIVKDRPLATLLLSAGHT
                     PDAEAAERLDQFFTEVRGLLVRAMESGMEIGLLRKVDPQLAAAAMLGMIRGVIEHLIR
                     EPNPQPIDEVVSEVLLVALRGVLRV"
     gene            8511..9455
                     /locus_tag="VMZ53_31675"
     CDS             8511..9455
                     /locus_tag="VMZ53_31675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33119.1"
                     /translation="MKAITVLGLTLGTSLASASPIKLVAPDASKVELAVYEHEGHAQA
                     QLTVQMSAPGVTTLRQPVTGTASITVPHGARFVGMSLRLGESSPSLATIMGNAIAERA
                     FTARVRRAWDPALLRQHTTSKKGDVYNLSVYPLTAERNATVELILELPDGTTFTPAKS
                     SAPFIEGVDASLVTITKPDGLLFASHTLRPVDANVALYADDPTPHRDSPVLVDVIPEP
                     NATSTAIALRPTLRRRLHELQYCFAREATNLGALELHFAVVPSGRVAAVSVDGAGEEA
                     NQCVSAALANVTFSGTSPFNDPDPETTVVSYPLMWIRT"
     gene            complement(9452..10237)
                     /locus_tag="VMZ53_31680"
     CDS             complement(9452..10237)
                     /locus_tag="VMZ53_31680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33120.1"
                     /translation="MRFAFAALMLAACTANVEMYDEPDLDADEELAMIEGDTSEPEQL
                     VEDTDTSEVTMIAKLACTKQRFLHVANYSFVGQLECVNGVCPNGCWGYQRRTSGFACD
                     YDATAADRVKTRDGGAAFATYNEIKPLHSDDDVAVASCRAQSGHKLRTYAVWNGAGWN
                     NEGIAAAVRFAELYGPQSEAMPDFWPWYNGYRGSWSPMNNVSPETRIDFEGVKRQIAK
                     MCSATRTGWVGTYFYDKDASGGSGMSDWKREAIIRGMNYCTTH"
     gene            complement(10293..>10547)
                     /locus_tag="VMZ53_31685"
     CDS             complement(10293..>10547)
                     /locus_tag="VMZ53_31685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33121.1"
                     /translation="FTSKVDPKLIKGPRLIVTGTEDRLIPAKVHRAMAAKYGAELREY
                     AQHGHYLMREPGWERIADDAIAWLHTVVGADLASAPASAS"
BASE COUNT         1877 a         3502 c         3477 g         1691 t
ORIGIN      
        1 gaagaagaag gaagaagtcg acaagttcat caagcaactt gccgattgcg cggcgaaggc
       61 cgacagcggc ggcaacgtgc agccgcaatc gctcgatcgt ccgaccatgc cgacgacgac
      121 gaccactccg acgacgacca ctccgacgac gacgacgacg aagccgaaca cgacgactcc
      181 gaccacgacg tcgacgaccc agaagccgac gacgacgacg acgactccga cgacgacgtc
      241 gtcgacgacg actccgacca cgacatcgac gactccaacg acgacgtcga cgactccgac
      301 gacgaccacg gcgacgacga ccacgccgac gaccacgccg acgacgacgg cacagctcga
      361 gaaccccgac gaggagcccg aggagcaaga gccttcggtt cgcgcgcaga cgggaacggg
      421 cgcgacgatg atctctgcgc gtgcagaagg cggcgttgcg ctgctcggcg gcaaggatct
      481 gaagttcccg gcgcaaccgg tgttcgcggt cagcggcgga tatccgcttg cggccggtcc
      541 ggcggtcgtc gagctcggag ctggcatctc gttctcgccg ctgccgtatc aggcgatggg
      601 tgagcaaaag cagggctcga tgatcggcgc gcgcgtgcaa gcggtcggcg cgtatccagt
      661 ggcgccgaag ctgtggctgc gcggcgagct gggcgtcggt gtcgtcgcgc ttggcggcct
      721 cgagatgggc aacccgctga cgaccgacgg cagcgcgaag tcgttcacgc tgctgagcgt
      781 gcgtgctggc gtggcggcgg actacgagat cacgccgaac atcgtcgcga ccctccagcc
      841 gttcgcgttt gcgttcagcc cgaagggcga cggcatcggc gcgttgcgcg agatcgacgt
      901 cctcgtcggc ctcggctacc gcaaataagc tctgccgctg tttcgctgcg cgattcgcga
      961 ctgcagcccg ctcgcgcgca gccggctcca cgagacgcgg cgaaacgcgc gcggaccgcg
     1021 catgccgctc agcgtctctg cgagcgcgag cgcgacgtga tgatcgagct cggcgagctg
     1081 atgtcgatcg gtcacgagct gtgcgaggag cggcgcgtag cgttgcgcga gaggcgagag
     1141 cggatcgagt gcctcgttga ccacgcggca cagcacgtcg aggcgagcag cttcgcctgc
     1201 atcgcgtgag atggcgtgcg tgcggcggat ccgtgcccgg acgtcgagca ggagctcgcg
     1261 ccatttgtcc cgcggcaatc gaatggtgcc gtcgaacgtg acctcgcagc cgaggaacgc
     1321 gatggccttg ctccccgcga agctcgtcga cgagcgaccc gcaccgttga agtagagcga
     1381 gcgcaccttg tgcgggctga gcgtcaagcc gagcttctcg atgcgtccac gcacctcgtc
     1441 gagcgcccac gtcgtcttcg cagcgtcgtc gtgcgcgaag atcaggtcgt cgccgaaccg
     1501 cgcgtagaag ccgccgggga cggccgcgag cgtctcgtcg atcgacgaca gatagagatt
     1561 gaggatcacg ttcgtgatcg gtgacccaaa cggcagcccg acaatgggcg aagcaggttg
     1621 ctcgtcggcg tcgagcaccg tcggtcgcag cagcgatcgc atgagcggaa gatcctcacc
     1681 gagcacctcg gcgagcagcg gccacagcgg cgcgtggtcg gcgagcggga cgctctcgca
     1741 gaaccgccgc acgtcggcgc gcagcacgta gaacccgcgg gtgcgcggat ccggtcgtgc
     1801 ccgccgatgc cgccgcgtga acagcgcgag gtcgcgcact gcctgccacg acgatcggcc
     1861 ggcgcgaaac gagaacaatc gcggcgacag gcgcgttgcg atcgcctcgt cgagaatctc
     1921 ggcagccacg gcgtggacga gcacgtcgaa cagcgagaac cgaacgagct ctcggtcctt
     1981 gtcgagccgc gcgaagtagc gaggcgccgg cgtgctgcga aagctgccgt cgcgcaccgc
     2041 gcgcgcgagg agcttggcga gcttgccctt gtcgttcacg aggtccgaca gcgggacgcc
     2101 atcctgttgc agctcgaaca cgcgacgcgt ctccgccacg cgcgcatata gtcgctcgag
     2161 ctgccgcaga tagcgggacg gttcgcccag cttgtccagg agcttcatgc gagggagcgc
     2221 ggaaggagaa gacggccctg aggggatgtt ggcgctacat ttggcgtctt gttcgcgaat
     2281 cgagaccgca gtctccggat ccgcgcaacg cagcggcttt caccgcgtgc cctcagggcc
     2341 aagacgatca gggcgggagt tcgatcaggg tattgctgga agccgcgcgg cagccaggag
     2401 tagcgcagta ggcactcgaa cgcgtttggt tcgtcaagct catgtatcgg ctcgggtatc
     2461 ggacggtgcg catgcgccac catccggcga cgtagggcgt gcccttcacc tccggctgat
     2521 tcgtgctggc gttgcgaccg acgcagaggc cgatgccgtt gatgtttagg atgaacacgt
     2581 cgcacatcgc aaagttgaag gtcaggttgc cgacgtggtg catctggctg aggttctggc
     2641 agctgccgcc ggccgcggcg tagttgcgct tcatcatcag cagcttgttg ctgttaccga
     2701 ggatgaaact gtggcgcagc gagaggtcct tgacgacgaa gtccttcggg aacgttcccc
     2761 aggtgctcgc ggagttcagc atcgaacgcg acgccttgac ctgcttgaac gggacgttga
     2821 tgaacgggtt cgcgatcttc agcgcggggt ccgccgtgaa gagaggcgcc gcgggaatcg
     2881 ggaacagcgc cgggttcgcg gtcgcatagt ttgccgccgc ggtgaagtac gccgtatcgg
     2941 tgggcgcgag cgggcacttc gccgcattgt tgaggtcgcg gtgcgcgtag aaggtgtaga
     3001 cggtcggcga gccgatcgcg acgccattct ggaggtagcg aacctcggtc ttgtacgtgc
     3061 catcggcctg tgccgcgggc ctgtaggtcg tcccgacgcc ggtggatcca tcacagggtg
     3121 cgaacgcggg cggtgtaccg gaggccaggt acgtgcggca ctcgtaggtg atgcccgtcg
     3181 cggtcgaggc aggcgaaagg gcgaacgtca tcgcgatcgg ttggttggtg ggccgcagcc
     3241 aacccgtcgg gctctcccat tgcgacgtga tcacgagcga tggcgcggcg gcacaaccgt
     3301 tctggcagag ctgagtcgca gaacagacgc cgcagccggt gccgcagtgc gcgcgatcgg
     3361 cggagacgtc gtagcaagta ccgccgcatg ccgtctggcc gccggtgcac tgacagctgc
     3421 cggtctgaca cgtcttgccg ccgctgcagg tggtgccgca tgcgccgcag ttagagttgt
     3481 ccgtcagctt gttgatgcag gcgccgttgc actcgaggat gttcgaccca gtgcacgcgc
     3541 agctgccggt ctgacacgtc ttgccgccgc cgcacacggt ggtgcagtta ccgcagtgcg
     3601 agttgtccgt ttgcgtgttc gcgcaaatcg ttccgcactg ggtttcattg gtgggacagc
     3661 cacagccgcc gttgatgcac gctgcgcccg caaggcacgc gtgaccacag ctgccgcagt
     3721 tgttgctgtc ggtcatcgtg tcgatgcacg tgccgctgca atccatttga ttggaaggac
     3781 agccgcacgt gccgtcgttg cacgtgaggc ccgtgccgca cgcgacgccg cagtcgccgc
     3841 agttcgcttc atcggtcgcg gtgtcgacgc agttggcgcc gcacagcttc ccgcccgacg
     3901 tgcattggca cgttcccgcc acgcactgct ccatggcgtt gcaagtgttg ccgcactcac
     3961 cgcattgctg gttgtccgac tgcatgtcgg cgcacgtgcc gtcacagtcg gtgagtcccg
     4021 acggacattc tcgagtgatg ttcccgcagc cgatcgctgc aagccccagc aggaaggcga
     4081 gccatagatg cttcattttg gcgaatcccc atccgaacgt attccattcg gagccacagc
     4141 gcagcaccag tttgcagctt cgacgcgggc acgccacgcg atccgctcct cacgagagga
     4201 gtcgcgcggc actgcgaaga gcaccttgat cacgcctgca ggccggaatg ccgtgatctc
     4261 gaacggcaga tctcgtcgac gacgggggcg gagtgcgtcg cgccacagcc gctttctcag
     4321 acgcggcggc agggccgcaa agcctgcagg tacgtccttg tcggcgagcg acgccacgag
     4381 accctgcggg aaacgctggc ggaactccgc catcagcgcc ttctcatcgg cgtgctcgcc
     4441 ctgcggctcg cggaggaggc ccgctgcgat cgcgcgttcc agcgcctgat gaccgtcggg
     4501 agcgacctct gtgacgtcga gcgcgcggcc aagtcccgcg gcgaggagtg ctcgctccgc
     4561 ggcgttgtcg acggagcctt cctcgaacca cagcaggcgg cgcgcgcgaa gtgtgacccc
     4621 gccgcgtcgg ccaagccgct ccgcccagcg ctccgccaag agtcgcggcg ccgtaccgcg
     4681 tgcctcgacc tgcagcacgc cgacccgggt agggcctcgc atctcgcgga gtgcacggac
     4741 ccagctctcg atgcctcggt ggacgcccgc ggtcgtatgc gtgcgccggc tgccaccgac
     4801 ggcgagaacg acggcgccga ccgagagagc agacgccagc atcaaccctg cggtcgcgtc
     4861 gtcgggggcg ccacgccaga tcgcgatgag gatggccacg caggttgcga ccgatccgat
     4921 gaacaaccgg atgaagcctc cgcgcgcgag ctcgcgcagc tggagcaccg acaggatcga
     4981 gagcgcgaga cacaacggtc cgagcgcggc gacgggctgg cgtacgagcg cgagaccgac
     5041 gcgccactgt gcgaccgcgg caacggcggc ccagatgacg agaccgacga tgatcgagac
     5101 gacccacagc cgccgttcga gcttgcgaca gagcgcggtc gcggcctctt cttcgagtcg
     5161 tttgaagtcg tgatagaaga cgcgcgacca gctcgtcgct gtggacaaga gcggcacggc
     5221 gagctgcagc acgtacggaa atgcgtcgag cttcgccatg tggaacggag cacgcacgaa
     5281 cgtcgcgagc agcatcgctg aaccaatgcg cgcggtgaga tttcccgccg cagcccacgc
     5341 gacctcgccc ttgtgcagct tctcgccgag ctttctcgac ggaaatcgca gtgacacgcg
     5401 tggcgggtgg agccgtgcaa cccgatacgc gcgcagcgtg tacgcgatcg tgagcccgcg
     5461 cgagatcaga atgttggcca atagcccaac ggggaatgcc cacgcaccga gcacgcgcca
     5521 gccgagtgcg acgacgagaa caccgagcgg ctcggcgagc atgatcgcca ccatcgggcg
     5581 gaagacgcgc tgcctcgccc acacgcccga atagagcgtg cgactgacga gatcgatcgc
     5641 gagccgcgcg acgcagacga gagcgtacgc ctcgaacacg ccgggcgcgc cgcgcgcgtg
     5701 ccaccgccac ccgagcagcg cgacgccggc gatgacgacg gcgaggccga gccacatcgc
     5761 cgcggagagc cagcgcgagg tctcctgcca cacgaacgcc ttcgtgccgt cggcgcggat
     5821 gcggcgccgc atcacctcga gcgcgcccca ccacaatccg tcgaacagcc gattgccgtt
     5881 ctggagcgcg accgacatcg ccaggtggcg ctgagagaac agcgccatca gcaccgtgaa
     5941 ctcgacgatg tgggtgatga gcccgaggcc tctggcgagc ccgacccatt tgagtcgaaa
     6001 cagggcgtag cgggcgaagc gcccgccgag gagcgcttct tccagcgaaa cgcgtcgctc
     6061 ggcggcacgc gcccgacgtc gcaggaatcg ctggaggtca tccacgatag gtgcgacccg
     6121 aaccataccc tggcggcggt cggaccaggc tcagattgcc gctaattgac cgatcgcgcg
     6181 aaccacgtta accgtaaggg atctagatgg cgcgccgccc cgttcgtcga cgttcgtttg
     6241 gccgccgacg tgcgcgtcgt gtgcggcaga tggaagccgt cggtttaccc gcgccgctcc
     6301 atacggtgtc gacacccgtc gatggtggtg cgctgcgcgt gctcggctcg ggcgatcagg
     6361 cgtaccaaac aattctcgag cgcgttcgca acgcggagaa gtccgtcgag attcgcgcgt
     6421 tcctttggcg cgacgacgaa gctggcaaca tgatggccga cgccgtgctg caagcggccg
     6481 atcgcggcgt gcaggtcacg attcagaaag atcgcatcgc ggccgtgtac gagtacaccg
     6541 gcggcaacaa gcagagcttc ttccacaagc gtgtcgatcc gatccgcggc attcaagcgt
     6601 ggttcctcgg cgcggtgacg cgcgcgaagg gcagcttcaa gcagaagcca aacgagctcg
     6661 cgctgcgaat cctccagcat ccgaacattc gcgtcgagca catgcgcaag cgcttcgatc
     6721 actcgaagct ctacatcatc gacgatcgca ttctcgcgct cggctcgatg ggcatcggtg
     6781 acaaccaccg ccacgagtgg ctggacgtca tggtcgaagc cgaaggcgaa gagtacgtcg
     6841 cgcggttgcg cgagcgcatg ctcggccacg acgagttcga tccatcgcgt ggcctcgact
     6901 tcctcgtgca cagccgcgag gcgcatcgca agaagagctg cccgatgatc agccatcgcc
     6961 tggcgctgat cgaagccgcg caggtgtcga tcaccgtcga gatggcgtac ttcggcgacc
     7021 gtcgcttcac ggcagtgctc gcgcgcgcag tgcagcgtgg cgtccaggtg aaagtcgtga
     7081 cggcagatcg cgcagacgtg ctcgcgaaca tcaaccgcgc cacgtgcgat gcgctgatga
     7141 agctcaccgg cgcgccgcag aatctgacga tcgtgctgct tccgcgcatg gtgcacagca
     7201 aggtcgtcgt catcgaccat cgctggtgcg acgtcggctc ggccaacttc acgtcgctct
     7261 cgcacggtgt ctacgacgag atcaatctgt acgtcgactg cgagccgttc gcgcgcgacc
     7321 tcgaagccga gatcgattcg cactgtctcg acggccgcat cgccgattcg cgcctgacgt
     7381 accgcaaggt cgcgtcgggt gtggagcgcg cgatcctggc gtaccagtcg cgacgaggcg
     7441 gctagtggcg acgacggcgg atcccagcga gcgcgcaaaa caagagaggc gccgtcagat
     7501 catgacggca gcaaaactcg tgttcgcgga cgccggctat cacggtgcgt cgatcaacgc
     7561 gatcatcgag aaggcgcaga tcgcgcgcgg cacgttctat ctctacttcg agagcaaggc
     7621 ggcggtgttc gacgccatcc tcgatcaagc gatgggcgag ctgcgttcgc gcattcatcg
     7681 catcgaagtc gtggacgcca atgcgccgcc tccgcaagtc cagctgcgcg agcagatcct
     7741 cgcgacgctc gattacatcg tcaaagatcg cccgctcgcg acgctgctgc tctccgctgg
     7801 ccacacgccc gacgccgagg cggccgagcg gctcgatcaa ttcttcaccg aggtccgcgg
     7861 cctgctcgtc cgcgcgatgg agtcgggcat ggagatcggc ctgctccgca aggtcgaccc
     7921 gcagctcgcc gctgccgcga tgctaggcat gatccgcggc gtcatcgagc acttgattcg
     7981 cgagccgaac ccgcagccga tcgacgaagt cgtcagcgag gtcctgctcg tggcgttgcg
     8041 cggcgtgctc cgcgtatagg ccgaaaaaac agccttgagc cggaaaaaaa agagcgcctg
     8101 ccggcgggca ggcgctcagc gcgaggagtc tgctaagcag gggcctcgcg aaaacctggg
     8161 tcgcttaacg ttgaccgagc tccggccgtc cgtcgagcga agatgtgtcg tcggtcttct
     8221 gagcggaatc atccgacgtc cgctggatgg tagggccacg cgcgccgccc ttcgacatcg
     8281 acgcacaagc agaggtggag agggcgatga cgaggaggag ggcgatccgc ttcatggctg
     8341 cgtcgagcac tattcaggga ccgcgccaat gtaggtagag ctgattccgt atacttactg
     8401 accgactgag gtggctagtc gcgtagccgg cgcctcgctg tcgatgcgcg ggattctcag
     8461 cagtccccag aatcgtgcaa ctcctcactt ccgctcgttg tcgaccgagc atgaaggcga
     8521 tcacggtcct tggtctgacc ctcggaacct cgcttgcctc cgcctcgccg atcaagctgg
     8581 tcgcgcccga cgcgagcaag gtcgagctgg cggtctacga gcacgagggt cacgcgcagg
     8641 cccagctgac cgtccagatg tccgcgccgg gcgtgaccac cctgcgccag ccggtgacgg
     8701 ggaccgcatc gatcaccgtt ccccacggcg cgcggttcgt cggcatgtcg ctcaggctcg
     8761 gcgaatcgtc gccatcgctc gcaacgatca tgggcaacgc gatcgccgag cgtgccttca
     8821 ccgcgcgcgt gcgccgcgcc tgggatccgg cgctgctccg gcaacacacg acgtccaaga
     8881 agggcgacgt ctacaacctc agcgtctatc cgctgaccgc cgagaggaat gcgacggtcg
     8941 agctcatcct cgagcttccc gacggcacga cgttcacacc ggcgaagagc tcggcgccgt
     9001 tcatcgaggg cgtcgacgca tctctcgtca ccatcaccaa accagacggc ctgctgttcg
     9061 cgagccatac gctgcggccc gtcgacgcga acgtcgcgct ctacgcagac gatccgacgc
     9121 cgcatcgcga tagccccgtg ctcgtcgacg tcattcccga gccgaacgcc acctcgacgg
     9181 cgatcgcatt gcgccccacg cttcgccgcc gcctccacga gctgcagtac tgcttcgcgc
     9241 gtgaagcgac gaacctcggc gcgctcgagc ttcacttcgc cgtcgtcccg agcggccgcg
     9301 ttgctgcagt cagcgtcgac ggggcagggg aggaagcgaa tcagtgcgtc agcgccgcgc
     9361 tcgcaaacgt gacgttctcc ggcacctcgc cgttcaacga tcccgatccc gagacgaccg
     9421 tcgtgagcta tccactcatg tggatccgca cttagtgcgt cgtgcagtag ttcatgccgc
     9481 gaatgatcgc ctcgcgcttc cagtcggaca tcccggagcc gccggacgca tccttgtcgt
     9541 agaagtacgt gccgacccaa ccggtgcgcg tcgccgagca catcttcgcg atctggcgct
     9601 tcacgccctc gaagtcgatg cgcgtctccg ggctgacgtt gttcatcggt gaccagctgc
     9661 cgcgatagcc gttgtaccaa ggccaaaagt ccggcatcgc ttccgactgc ggcccgtaga
     9721 gctcggcgaa ccgcacggcc gccgcaatcc cctcgttgtt ccagccggca ccattccaga
     9781 ccgcgtaggt gcgtagcttg tgcccgctct gcgcgcggca actcgcgacc gcgacatcgt
     9841 cgtccgaatg cagcggcttg atctcgttgt acgtcgcgaa cgccgctccg ccgtcgcgtg
     9901 tcttcacgcg atccgcagcc gtcgcatcgt agtcgcacgc aaagccgctc gtccgccgct
     9961 gataacccca gcagccattc ggacacacac cgttcacgca ctcgagctgg ccgacgaagc
    10021 tgtagttcgc gacatgaagg aagcgctgct tcgtgcacgc gagcttcgcg atcatcgtca
    10081 cctcgctcgt gtcggtgtcc tcgacgagct gctcgggttc gctcgtatcg ccctcgatca
    10141 tcgcgagctc ttcatctgcg tcgaggtccg gctcgtcgta catctccacg ttggccgtgc
    10201 acgcggcgag catcagtgca gcaaaggcga agcgcatggc cgtcccagac tgcaaggcca
    10261 agaccgcggg agctgtgcgc gatcgcggcc agttagctcg cgctggctgg ggcactcgcc
    10321 aggtcagcgc caacgaccgt gtgcagccaa gcaatggcat cgtccgcgat gcgctcccaa
    10381 ccgggctcac gcatgaggta gtggccgtgc tgcgcgtact cgcgaagctc tgcgccgtac
    10441 ttcgccgcca tcgcgcgatg cactttcgcc gggatcagtc ggtcttccgt gcccgtcacg
    10501 atcaagcgcg gtcccttgat gagttttggg tcgaccttcg acgtgaa
//
LOCUS       DATLBZ010000644         7255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10181073, whole genome shotgun sequence.
ACCESSION   DATLBZ010000644 DATLBZ010000000
VERSION     DATLBZ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10181073"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /gene="thrC"
                     /locus_tag="VMZ53_31690"
     CDS             <1..1262
                     /gene="thrC"
                     /locus_tag="VMZ53_31690"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HUS33122.1"
                     /translation="RCPQCGGLLEVVHDMAALKTRSAGEWKSLFAERWRSVEEPFGSG
                     VWGKHEWVAPQLEPKNIVSMLEGVTHLTRVPGYAKELGLADVRVKQCGTSHSGSFKDL
                     GMTVLVSMVKQMIARGAPIRAIACASTGDTSAALAAYAAAAGIRSVVILPRGKISTAQ
                     LVQPLAMGSLVLAIDTDFDGCMRLVQELASKEGVYLANSMNSLRLEGQKTVAIEIAQQ
                     LGWEVPDWVVIPGGNLGNVSALGAGFEMLLALGLIDRRPRICVAQAAHASPLHTSYKA
                     SWADLQPVKARTTLASAIQIGNPVSFKKAVRALRAFEGVVEAVSEAELADASARVDRY
                     GLFTCPHTGVALGAVEKLAARGEIKKDERVVVISTASGLKFADFKVGYHEGKLAEVAA
                     PRHRNIPVELPEDYGAVREALLRGLDR"
     gene            1334..1912
                     /locus_tag="VMZ53_31695"
     CDS             1334..1912
                     /locus_tag="VMZ53_31695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS33123.1"
                     /translation="MHAAARVRPVEPIDELTLRRAQRGDERAWRDLIARFQPPVHALI
                     WRLLAGRARHRVEDLTQETFVRVLKALPKFDPSGPATLGTWILTIATRLTLNELRRPE
                     TSVLERDVIGRERTDADAERRRLGAAIAQGIAALPDAQRAVLVLREYHDLDYGDIADA
                     LEIDVGTVKSRLSRARVALREYLTRELGGRYE"
     gene            1905..4538
                     /locus_tag="VMZ53_31700"
     CDS             1905..4538
                     /locus_tag="VMZ53_31700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33124.1"
                     /translation="MSDDNALKDIDLSAWDVPSPETSADDLADSVIARVGGTEVGAAI
                     PVDDVPLGGRKRALLIAGVAAAAIAVTLGVWVIARGARHAAPKDGAVIAEKAQALELD
                     AAHADLDPGADIRWSRKGSVLRVEQRAGSAAWRVGTDGKLAIDAGNTGAVLASIEATG
                     ASLRVEVKPMNTADVKVMGAIATTAAVTALVTVVVYEGHVKVRDNGTQQTVIVQPGTT
                     YTITPPPPSPEPFPVADDDSDDTSVVGVAPVGADQPPEVSIESGEDVFIHDMAPPTRV
                     KIDTSKACRNGKVELVVNGVSTSSDTLAFNRGNMTYHVMCDTNDVGSGTVVVTRDPLS
                     RPLPSPPPVNSVTADGRVYRVTYQQMPAIEVHGGPGTLHLSQGSVEKTYEARGDSVLI
                     PSSDLAQGDYRFWFTPDKMTTLTLALDTTAPVVSLEPIVWGPQIELEGFVLAGSALMI
                     DGVPVPVGSGRFRASVPGGSDTLILRIDHASAGTHYAIARQNGTTAQPNPVTVSQSPN
                     SLDRSSITKVMASLKTKFAACVQQHPQTTRGIVKVKVVVKTDGSVNRVDVSQGVEAKL
                     DECMRTAILPAKFPNATTETTFSYPITVEPTPIAVVPPANCDADAYKDKGMQNVNMGQ
                     HAAALAQFEASLRCKPDAYVQQLAYMSACNSQNAAKAVVYWKKMTKTQQEKYEPMCVR
                     NHIDIRALAKGDDTDLTPPTCDADATKDKGMEWVNLGQHATALKLFEQSLACKKDAYV
                     VALAFMAACNAQDADKARKYYAQLTPPQQTKYKIMCIANHIDPAASPSDSESDTDPDP
                     FVTRPPSAVAQGYLQVFSKPKAKVFVDGVDSGKTTPIKGKNLALTPGKHKITFLIGDD
                     RFTYPVVIQSGKTETMTKDLQ"
     gene            4563..7091
                     /locus_tag="VMZ53_31705"
     CDS             4563..7091
                     /locus_tag="VMZ53_31705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33125.1"
                     /translation="MRALAFVLLCTSALAACGDDAPSNGVTTDVCSYVPVQATAHAGG
                     TVTAQPLQAGAAERVLDVPVGTALGGYTSRAGFLGSAGVVDARKVKISGSFNPSIGVT
                     TAPKVKAVALTAGDETVVILHFDAIFVYEGMVYDLEQRLGAEYAGKVIIAASHSHSGW
                     AQFTGHGPLKLGSGQMRQLVYDRFMASFEGAARDAIAARRAAKIGFFFDGNFDPMNVI
                     NHDRRGDNDMLPGGNKKDDHFYFIRIDGTDNAPIAAIPIFGEHGTLNDDDNPFASTDA
                     TGALERTFQEEFDSKVVVMHMQSAGGDNSPNGHGGIDCNNKPGNPGDPCFLWASEEGH
                     GRGAVPALMAAWTAAGANMRDSVELEMLTRSVETGPKAGTFAIRDGALKYAPFDLKKE
                     PDGVIYDSTGAVQSPIDEFNAPVGAGLCEKPDAMFPAANIPGTEGLTPYGSCLRLDAA
                     GEILGEIFSIDFGVTETHPVCEATRTTISALRIGDYVIGTMPGELTVMLATYLRSKSP
                     VAEDHTILLGYAQGHVGYMLRPEDWLKGGYEPSVTFWGPLEAEYIGEQLLKLMPLAMT
                     PAREDAASASATRVAVATMTDGLEIDDPAPEKGTVPATVPNTVWARTGHPTQAQPLSS
                     IPRISGIATFVFVGDDPKTKTPHITLQYEPTLNSGTFVEVTRRSGRVVDDAEVILAYT
                     PDPLVRTGPQHHYWVVEWQALPWLGAPGADGLDQRGNVPTGRYRFHVEGSGWTLDSAP
                     FTVVEGGLTATATRSNGQIHVAVKWHAPKGWRLMDFALMSNQPVPVKSQQVTIELRNS
                     GGALGSATMPSTDGNGNVDVTDNGGATIVRVTDRFGNTFDATLP"
BASE COUNT         1299 a         2352 c         2434 g         1170 t
ORIGIN      
        1 atcgctgccc gcagtgcggc ggcttgctcg aggtcgtgca cgacatggcc gcgctgaaga
       61 cgaggtccgc cggcgagtgg aagtcgctgt tcgccgagcg ctggcgttcc gtcgaggaac
      121 cgttcggctc cggcgtgtgg ggcaagcacg agtgggtcgc gccgcagctc gagccgaaga
      181 acatcgtctc gatgctcgag ggcgtcacgc atctcacgcg cgtgcctggc tacgcgaagg
      241 agctcggcct cgcggacgtt cgcgtcaagc aatgcggcac gtcgcactcc ggctcgttca
      301 aagacctcgg catgacggtg ctcgtgtcga tggtgaagca gatgatcgcg cgcggcgcgc
      361 cgattcgcgc aatcgcatgc gcatcgacgg gcgacacgtc cgccgcactc gccgcatacg
      421 cagccgccgc cggcattcgc tcggtcgtca tcctgccgcg cggcaagatc tcgacggcgc
      481 agctcgtcca gccgctcgcg atgggctcgc tcgtcctcgc gatcgacacc gacttcgacg
      541 gctgcatgcg tctcgtgcag gagctcgcct cgaaagaagg cgtctacctc gcgaactcga
      601 tgaactcgct gcgcctcgaa ggtcagaaga ccgtcgcgat cgaaatcgcg cagcagctcg
      661 gctgggaagt ccccgactgg gtcgtcatcc ccggcggcaa cctcggcaac gtgtcggcgc
      721 tcggcgctgg cttcgagatg ctgctcgccc tcggcctcat cgatcgccgc ccacgcatct
      781 gcgtcgcgca agccgcacac gcctcgccgc tgcacacgag ctacaaagca agctgggccg
      841 atctccagcc ggtcaaggcg cgcacgacgc tcgcgtccgc gatccagatc ggcaaccccg
      901 tcagcttcaa gaaagcagtg cgcgcgctcc gcgcattcga aggcgtcgtc gaagcggtca
      961 gcgaagccga gctcgccgat gcatccgcgc gcgtcgatcg ctatggcctc ttcacgtgcc
     1021 cgcacaccgg cgtcgcgctc ggcgcggtcg agaagcttgc cgcgcgcggc gagatcaaga
     1081 aggacgagcg cgtggtcgtg atctcgacgg ccagcggtct gaagttcgct gacttcaagg
     1141 tcggctatca cgagggcaag ctcgcggagg tcgctgcgcc tcgccatcgc aacatccccg
     1201 tcgagctgcc cgaggactac ggcgccgttc gcgaagcgct gctgcgcggc ctcgaccgct
     1261 gatttcacgc ggttacgcgt tcgcgcgcat tgcgtggaac cttttgggcg ctgccgggtg
     1321 ataccggtag cccatgcacg ccgccgcacg cgtccgtccc gtcgagccaa tcgacgaact
     1381 gacgttgcgc cgtgctcaac gcggtgacga gcgcgcgtgg cgtgatctca tcgcgcggtt
     1441 tcagccaccg gtgcacgcgc tgatttggcg gttgctcgcg ggcagggcgc ggcatcgcgt
     1501 cgaagatttg acgcaagaga cgttcgtgcg cgtgctgaag gcgctaccga aattcgatcc
     1561 gtcgggacca gcaacgctcg gcacgtggat cctgacgatc gcgacgcgcc tgacgctcaa
     1621 cgagctgcgc cggcccgaga cgagtgtgct cgagcgcgat gtgatcggac gcgagcgcac
     1681 ggatgcggat gcggagcggc ggcgattggg cgcggcgatc gcgcaaggca ttgcggcgtt
     1741 gccggatgca cagcgcgcgg tgctcgtgct tcgcgagtat cacgatctgg attatggaga
     1801 cattgccgac gctctcgaga tcgatgtcgg cactgtgaag tcgaggttgt cgcgtgcgcg
     1861 ggttgcgctg cgcgagtacc tcacgcgcga gctcggaggc cgttatgagt gacgacaacg
     1921 cgctgaagga tattgatctg tcggcgtggg atgtcccgtc gcccgagacg tcggcggacg
     1981 acttggctga ttcggtgatc gcgcgcgtgg gcggcaccga ggttggcgcg gcgatccccg
     2041 tcgatgacgt gccgctcggc ggtcgcaagc gcgcgctgtt gatcgcgggc gtggcagcag
     2101 cggcgattgc cgtcaccctg ggcgtttggg tgatcgcgag gggcgcgcga catgcagcgc
     2161 cgaaagacgg cgcggtgatc gcggagaagg cgcaggcatt ggagctcgat gccgcgcatg
     2221 cggacctcga tccgggcgcg gacattcggt ggtcgcgcaa ggggagcgtg ctgcgcgtcg
     2281 agcagcgagc gggaagtgcg gcgtggcgcg tggggacgga cggcaagctg gcgatcgatg
     2341 ccggcaacac gggggccgtg ttggcctcga tcgaggcgac aggcgcgagc ctgcgcgtgg
     2401 aggtgaagcc gatgaacaca gcagacgtta aggtcatggg ggcgatcgcg accacggcag
     2461 cagtaacggc gctcgtcacg gtcgtcgtct acgagggcca cgtgaaggtg cgcgacaacg
     2521 gcacgcagca gaccgtgatc gtgcagcccg gtacgacgta cacgatcacg ccgcctcctc
     2581 cgtcgccgga gccattcccg gtcgccgacg acgattccga tgacacgtcg gtggttggcg
     2641 tcgcgccggt cggtgcggat cagccgcccg aggtgagcat cgagtcgggc gaggacgtct
     2701 tcattcacga catggcgccg ccgacgcgcg tgaagatcga tacgtcgaag gcgtgtcgca
     2761 acggcaaggt cgagctcgtc gtcaacggcg tctcgacgtc gagcgatacg ctcgcgttca
     2821 accgcggcaa catgacgtat cacgtgatgt gcgacaccaa cgatgtcggc agcggcaccg
     2881 tcgtggtgac gcgcgatcca ctgtcgcgcc cgctgccctc gccgccgccg gtgaattcgg
     2941 tgactgcgga cggccgcgtg tatcgcgtga cgtaccagca gatgcccgcg atcgaagtgc
     3001 atggcggtcc aggcacgctg catctctcgc aaggttccgt cgagaagacg tacgaagcgc
     3061 gcggcgatag cgtgctgatt ccgtcgtctg atctcgcgca gggcgactat cgcttctggt
     3121 ttacgccgga caagatgacg acgctgacgc tcgcgctcga tacgacggcg ccggtggtct
     3181 cgctcgagcc gatcgtgtgg gggccgcaga tcgagctcga gggcttcgtg ctcgcgggca
     3241 gcgcgctcat gatcgatggc gtgccggttc ccgtcggtag cggccgcttc cgcgcgagcg
     3301 tgccgggcgg cagcgacacg ctgatcctgc gcatcgatca tgcgagcgcc gggacgcact
     3361 acgcgattgc acggcagaac ggtacgaccg cgcagccaaa tccagtgacg gtctcgcagt
     3421 cgccgaattc gctcgaccgg tcgtcgatca cgaaggtgat ggcgtcgctc aagacgaagt
     3481 tcgcagcgtg cgttcagcag catccgcaga cgacgcgcgg catcgtcaag gtgaaggtcg
     3541 tcgtgaagac ggacgggagc gtcaatcgcg tcgacgtctc gcagggcgtc gaagcgaagc
     3601 tcgacgagtg catgcgcacg gcgatcctgc cggcgaagtt cccgaatgct acgaccgaga
     3661 cgacgttctc gtacccgatt accgtcgagc cgacgcccat cgcggtggtg ccgccggcga
     3721 actgcgatgc cgacgcatac aaggacaagg gcatgcagaa cgtcaacatg gggcagcacg
     3781 ccgcggccct cgcgcagttc gaagcctcgc tgcgctgcaa accggacgca tacgtccagc
     3841 agctcgcgta catgtccgca tgcaactcgc agaacgctgc caaggctgtc gtctactgga
     3901 aaaagatgac gaagacgcag caggagaagt acgagccgat gtgcgtccgc aatcacatcg
     3961 atatcagggc gctcgcaaaa ggcgatgaca cggatctcac accgccgacc tgtgacgccg
     4021 acgcgacgaa ggacaagggc atggagtggg tgaacctcgg ccagcacgcg accgcactga
     4081 agctgttcga gcaatcgctg gcgtgcaaga aggacgcgta cgtcgtcgcg ctcgcgttca
     4141 tggcggcgtg caacgcgcag gacgccgaca aggcgcgcaa gtactacgcg cagctcaccc
     4201 ctccgcaaca gaccaagtac aagatcatgt gcatcgcgaa ccatatcgat ccggcggcgt
     4261 cgccaagcga cagtgaaagc gacaccgacc ccgacccgtt cgtgactcgc ccgccctccg
     4321 ccgtcgcgca gggctacttg caggtctttt ccaagccgaa ggctaaggtc ttcgtcgatg
     4381 gtgtcgacag cggtaagacc acgccgatca agggaaagaa tctcgcgctc acccctggta
     4441 agcacaagat cacattccta atcggcgatg accgcttcac gtacccggtc gtgatccagt
     4501 cgggcaagac cgagacgatg accaaagacc tgcagtagac gaccgtgtta tgccagccct
     4561 cgatgagggc tttggcattc gtgcttttat gcacatctgc gctcgcggcg tgcggtgacg
     4621 atgcgccgtc gaacggcgtg acgaccgacg tctgcagcta cgtaccggtg caggcaactg
     4681 cgcatgcagg tggcacggtc accgcgcaac cgctgcaagc aggtgcggcg gagcgcgtgc
     4741 tcgatgtgcc tgtcggcacg gcgctcggcg gttacacatc gcgcgcgggc tttctcggca
     4801 gcgccggcgt tgtcgacgcg cgcaaggtga aaatctccgg ctcgttcaat ccgtcgatcg
     4861 gtgtgacgac cgcgccgaag gtgaaggcgg tcgcgctgac tgcaggcgac gagaccgtcg
     4921 tcatcctcca cttcgatgcg atcttcgtct acgagggcat ggtctacgac ctcgagcagc
     4981 ggctcggcgc cgagtatgca ggcaaggtca tcatcgccgc gtcgcactcg cactcggggt
     5041 gggcgcagtt cacgggccac ggcccgctga agctcggctc cggccagatg cgccagctcg
     5101 tgtacgaccg gttcatggcg tcgttcgagg gtgcggcgcg cgatgcgatt gcggcacgtc
     5161 gcgcggcgaa gatcggcttc ttcttcgacg gcaacttcga tccgatgaac gtgatcaacc
     5221 acgatcgccg cggcgacaac gacatgctgc ccggcggcaa caagaaggat gatcacttct
     5281 acttcatccg catcgacgga acggacaatg cgccgatcgc ggcgattccg atcttcggcg
     5341 agcacggcac gctcaacgac gacgacaatc cgtttgcgtc gaccgacgcg accggtgccc
     5401 tcgagcgcac gttccaagaa gagttcgaca gcaaggtcgt cgtcatgcac atgcagagcg
     5461 ccggcggtga caactcgccg aacggccacg gcggtatcga ctgcaacaac aagccaggca
     5521 atcccggcga tccgtgcttc ctgtgggcaa gcgaggaagg tcacggtcgc ggcgcggtgc
     5581 cggcgctgat ggccgcgtgg acggccgcgg gcgcgaacat gcgcgacagc gtcgagctcg
     5641 agatgctcac gcgctccgtc gagactggac cgaaggccgg cacatttgcg attcgcgatg
     5701 gcgcgctgaa gtacgcgccg tttgatctga agaaggaacc cgacggcgtg atctacgact
     5761 cgacgggcgc cgtccagtcg ccgatcgacg agttcaacgc gcccgtcggt gctgggctct
     5821 gcgagaagcc cgacgcgatg ttcccggctg caaacattcc aggcaccgag ggcctcacgc
     5881 cgtacggctc gtgcctccgc ctcgatgcgg cgggcgagat cctcggcgag attttctcga
     5941 tcgacttcgg cgtcaccgag acgcacccgg tctgcgaagc gacgcgcacg acgatctccg
     6001 cgctgcgcat cggcgactac gtgatcggca cgatgccggg cgagctcacc gtcatgctcg
     6061 cgacgtatct gcgcagcaag tcgccggtcg ccgaggacca tacgatcttg ctcggctacg
     6121 cgcagggtca cgtcggctac atgctgcgtc ccgaggactg gctcaagggc ggctacgagc
     6181 cgagcgtcac gttctggggc ccgctcgaag ccgaatacat cggtgagcag ctgctgaagc
     6241 tgatgccgct cgcgatgacg ccagcgcgtg aagacgctgc gagcgcgagc gcaacgcgcg
     6301 tcgccgtcgc gacgatgacc gacggtctcg agatcgacga ccccgcaccg gagaagggca
     6361 ccgtgcccgc gacggtcccg aacacggtgt gggcgcgcac cggccatcca acgcaggcgc
     6421 aaccgctctc gtccatcccg cgcatctccg gcatcgcaac gttcgtcttc gtcggtgatg
     6481 atccaaaaac caagacgccg cacatcacgc tccaatacga gccgaccctc aacagcggga
     6541 cgttcgtcga ggtcacgcgt cgcagcggtc gcgtcgtcga tgacgccgaa gtcatcctcg
     6601 cgtacacgcc ggatccactc gtgcgcacgg gaccgcagca ccactactgg gtcgtcgaat
     6661 ggcaggcgct gccgtggctc ggtgcgcccg gcgccgacgg tctcgatcaa cgcggcaatg
     6721 tgcccacggg tcgctatcgg ttccacgtcg aaggtagcgg ttggacgctc gacagcgcgc
     6781 cgttcacggt cgtcgaaggt ggcctcaccg caactgcgac gcgctcgaat ggtcagatcc
     6841 acgtcgccgt gaagtggcac gcgccgaagg gttggcgcct gatggacttc gcgctgatgt
     6901 cgaaccagcc cgtccccgtg aagagccagc aagtcacgat cgagctccgc aactcgggtg
     6961 gcgcgctcgg ctccgcgaca atgccctcga ccgacggcaa cggcaacgtc gacgtgacgg
     7021 acaacggggg cgcgaccatc gtgcgtgtca ccgatcgctt cggcaacacg ttcgacgcga
     7081 cgctcccgta gcggtaagac cacttccgtc gtggatacgc ggcacctcgg ggcgtgaagt
     7141 gctgtccacg gctgcgttcg gcggagctcc atgatttcgg gcgattaggc ctggccgagg
     7201 cgttgcacta gctcggggcg tgcgcctctt gttgctcagt ctcgtacccg ttgcc
//
LOCUS       DATLBZ010000645        26944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3614730, whole genome shotgun sequence.
ACCESSION   DATLBZ010000645 DATLBZ010000000
VERSION     DATLBZ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 26944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26944
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3614730"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="VMZ53_31710"
     CDS             <1..2008
                     /locus_tag="VMZ53_31710"
                     /inference="COORDINATES: protein motif:HMM:NF015114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS33126.1"
                     /translation="HAQPADVPGLIKLIENQPSDMDRSTWKEKRRDAARKLGQSKDKR
                     ATPVLVRLAETETFDIIGDIAIEGLGYLNDQSAVPALQKVLADTSRDKSSRDLAKKSL
                     GKLGAPDKPVGGTTGTTGTTGTTGTTGTTGPTGGTTGTTGTTGTTGATGRGTGKTGGT
                     GGTTGSTGTGKTGGTGGTGGTGTGGSITGGTTGTGTTGGTTSGGTATGTTTTVGSSGT
                     TTGTGSITTGIEGGGETGGSTGGGDLTVGLGGGNASAVEGMPEMNDDVIAASERLTFA
                     AGTANLQIQTTPNDNNGNRNKHINFDTDVASNYQRRMDAESRAFALDGDVKLVGAFNS
                     QQGRGSSKGVLFNTRIGGEARFYSNKLYGIGKAAGALSIQYINYRDPNDNTGGNDLKD
                     TTFAVDLQIALGGGFGRVVDIGSQIRVHRLSRILDDSRALGKPIDAATSKKLQLTWWA
                     LRKERSQFRSLIATIAVLREAGILLGEPNAVITYQILSVLQDTSLFLRPSGFDVQLVV
                     GEGYLKRPTDDAGNCLVTCGRLEQVIASAAYAQQVDDDKLEISGGGYARYRLFAPDDG
                     TPTPWAIGASAGMRRFTYGQHGDVFGVFDLRGGLALSSDGSADPNNPSDKALRIEGSL
                     GFTYVLNQASGIRLAAEVAEDQGTIYLGASLQATYGLLDGTYAR"
     gene            2074..3978
                     /locus_tag="VMZ53_31715"
     CDS             2074..3978
                     /locus_tag="VMZ53_31715"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HUS33127.1"
                     /translation="MSDLSAQQHETLLAVAETALPAGRFIPGANAATVEKVERFVEKL
                     PSQLSFGLKGLLRGLDAASWLGERRPFRKVKPAKRLALLESWRQGDPIRRLMLRALVS
                     PLKVAHFDDPALYKQLGCVYDVEKAKAEAKPAYMRDRVHRADELDGDLAIECDVVVVG
                     TGAGGAVVGRELAEAGLAVVFVEEGHYYERTQFNGRAIDMQQRLYRRAGSTFSVGNVG
                     IPIPLGQSVGGTTTINSGTCFRTPDRILHHWQKDLGLDELGPEHMGPYFDRVEAVLGV
                     EPARPELLGGNGRVIQRGCDALGFTRHGPLKRNAPACDGQGVCCFGCPTDAKRSTNVS
                     YIPLALRAGAELFAGAKMTRVIVDGGRARGIVATTTKGHVLTVRSRAVVVACGSIMTP
                     IVLGAQGLGGRSGQLGKNLSIHPACGALAEFDEQIMPWKGIPQGYSIEELHEEGILFE
                     GAMVPLEMTMNMTQMIGPDLIRLAESFDRVASFGFMVEDTSRGSVREVMGQPVIQYWL
                     QDKDVSHIKRGVDVLSQIFFAAGARRVHTPIAGFDVLESEADLVNLRRANIRAWDLDL
                     SAYHPLGTARMGADPASSVINANHEMHDTQGLFIVDGAAVPTALGVNPQITIMALATR
                     AAEKIAAQLT"
     gene            4054..4626
                     /locus_tag="VMZ53_31720"
     CDS             4054..4626
                     /locus_tag="VMZ53_31720"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUS33128.1"
                     /translation="MRTALAAASTLAAVALSCLPGLAEARPFTAGVGVGRSQAKADAE
                     GDASDTLQLFGRFSFTKRLSAQLEIQKLQLPYGDVTVRSGTLLLVVDLGQRGHLVPIL
                     MAGFGGASATDDYGYETSASHTEGGAGLEYRADGGLTIGVDLRIGGRSLDDDDVKIQP
                     ATTDVIAIYPYNSLQAGEYRSARLYAAVRF"
     gene            complement(4623..6074)
                     /locus_tag="VMZ53_31725"
     CDS             complement(4623..6074)
                     /locus_tag="VMZ53_31725"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HUS33129.1"
                     /translation="MSHVRPEKWADAFAGKLATSVVAQMEAHADACKRCATARDRVES
                     ASQSFPALRAQQAPDVAWDTVRARIHWSVSSEKRARDRAPRERHRAIWPLAGAGILGA
                     AALTLVLVTGSGSSTPKQPVATNDQAPIAVTLVSPPAPVTGVVSRLAGQVMIDGVRRA
                     DAFERTLAVGNVIATGDGRIDVQFGADSAFALGPRSTLELHRFDAERIELKVEGTVDI
                     TVAPRVAGQRFVVIAGDQTVEVRGTQFRVVHDGASTQVACRHGKVIVTDSHGTAEVAT
                     ARKLVTTQDAPLDPQHVRALTVDELDQLAQATPAMTPIWTDADSLVRTSAPLEISTTS
                     KRAVRVDGVELGDAPLLVRVMPGRHTVETADAAGRFRRAGWVDIAAAKPARLVVQPDA
                     APTGGTAERRRQLHAGIDQRRLAQCTRAITKAGLASYVHVEIAIDETGAVGFLNIIDT
                     DLSSSTASCVHDVLADVRFKPGASATFRDKIDL"
     gene            complement(6071..6688)
                     /locus_tag="VMZ53_31730"
     CDS             complement(6071..6688)
                     /locus_tag="VMZ53_31730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUS33130.1"
                     /translation="MKRETGSAAALDPADEADLIERCRRDDRAAQDELYHRFRRQVAG
                     NLYRVLGDRTDLDDLVQEVFVIAFRGLDRFRGDARLSTWLYRICVNVALGRIRTRRRR
                     PAAIGMVDLDSAAVDPSLTERPETPERSLQRREDQERVYRALDSLAPKKRVVLYLHEI
                     EGLDLKEIAYLVDSNPVTVRTRLFYARREFYKVLAGEPADDGGDE"
     gene            complement(6774..7916)
                     /locus_tag="VMZ53_31735"
     CDS             complement(6774..7916)
                     /locus_tag="VMZ53_31735"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS33131.1"
                     /translation="MGLLVATPAAADTFGGFSGVDKPFLVNQDRVCQPLAVDKGAAKG
                     TPTCEKATADVVAQLSIKEPIIQRGPKAVFAATISGKNLTVTKDGTAVVTWTAVDPLG
                     KVVEVYSSQYEDRVAVAYTQRVLGKEVTSVVAFELVKTTGKTEPIKPPDTTTTTTTTT
                     TTTTAAPADDPKVTKAIAAAKKAPKAKAVAAWQGVLAMAPDHSEAMYRLATAQIANKA
                     NGDAIATLDKLAKSSRSDAIEWLVEARFEKAFAAVRADPKYRAAVGLDRKPTTPYERL
                     MGFGGQWEQSMTSCEAPEVHLTVSRDRAFKVTVKTMCQGEGFSISKKGTWRIEGDGVV
                     MTVPTKGAKTTAKDEFPCDFEAKKDEDALHCVLDRDLDFTVLPTRR"
     gene            8053..9093
                     /locus_tag="VMZ53_31740"
     CDS             8053..9093
                     /locus_tag="VMZ53_31740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33132.1"
                     /translation="MVRFMERRMAPSGPRIDPGDARTRLIELADHYSDGTLGHPSPFF
                     PEPDVPDIKMAHQGDGPLGTQVVDLSFASSYRPYHPLARDPYLAVTENMTAHARWWTS
                     DRGRPTIVLIHGWGAGSHWMTERAFQLPYWLRHGYDVLAFQLPFHGNRAPAGIGKSGA
                     LFPSANPLRTNEGFGHAIYDLRALARFIRSRGASAVGVMGMSLGGYTTALWTTIAGPG
                     DVGGVDFAVAMIPAVSMSRLFWQHGEDSPVRRRASKAGITEDLLAEAFAVHAPTTRAP
                     RLPKDRLYVIAGRGDRITPPDQAEALARHWDREITWFDGGHLTQVGRGDALRTVRRSL
                     GAHAFPGQAFRG"
     gene            9086..10966
                     /locus_tag="VMZ53_31745"
     CDS             9086..10966
                     /locus_tag="VMZ53_31745"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HUS33133.1"
                     /translation="MADTSKPLAEVLATGPLLLDGAMGSLLYERGVLHTRSYDELNLS
                     NPELIRTVHHDYVQAGAEIIETNTFGANRMALARHGLVEQGQAINRAGVELARKAAGD
                     RAYVAGAVGPTGVKFHIATAAERRLARFALAEQIDTLVLAGADAIILETFTSILEMEE
                     AIRVCKERGPKVPVLAMMVFDGQSRSDGGLGPAEIADRMIAAGADVVGGNCGIGPAEL
                     YQVVTNMVGRGKPVIAQPNAGLPASVEGRTLYVANPEHFGVFARRMLKSGVRLVGGCC
                     GTTPEHTRAMLGAVRMLRGEKVDELGTKSSPTIQISPGPAPAAKIALPDRSRMGGRIA
                     RGEFVVSVELTAPTGLDMTKTKQQVTQLIEGGVDIVNIADGPRASARMANVAVCARLA
                     SETNVEPILHVCARDRSFLGLVAHLLGAHGLGLRNLVIITGDPPKMGDYPFSTPVYDV
                     DSVGLLRMAAGLNAGVDPAGKECEPTHFTLATGAEPAAHDRERELRRLEEKKLAGAEL
                     VMTQPVYDPRTLERFLDDAAPLGLPVMVGILPLASHRNAEFLHNEVPGMSIPAEYRER
                     MAKVGGGPAARVEGIKIAQEALGAVKNRVAGAYVMPPFNRVESALTVLEVARDRWKPG
                     AR"
     gene            10963..11994
                     /locus_tag="VMZ53_31750"
     CDS             10963..11994
                     /locus_tag="VMZ53_31750"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HUS33134.1"
                     /translation="MSLTLDEARALHDEVCVIDLHADTPKLMDKLGYDFMARHDTPMP
                     RRANILGHVDVPRLREGGMAAQFLALWTWPKYVPGAERSNIRSVLNQLDAVQSVVDKN
                     PEQLAWTRTGAEIRAAKAAGKIAVLGGIEGGHALEGKLEAIEMFARRGVRYLGPIHLW
                     PSAYGSSSKQPKKDDGLTALGIDVIRECERTGVVVDLSHMSHKSYAQALEVVKLPPMV
                     SHTGVLGVHEHWRNITDEQIREVANRGGCIGVIFARNYLGGKSIDSVVDHILHVINVA
                     GEDVPALGSDFDGFVVPPEGLEDIAAMPNLTVALSRRGVAPRVLEKILGGNVLRVLDA
                     IPAWGKLAA"
     gene            11991..12749
                     /locus_tag="VMZ53_31755"
     CDS             11991..12749
                     /locus_tag="VMZ53_31755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33135.1"
                     /translation="MKAIVALLLACAGCDAVFSVDHVREQLTPDADVCAQGDEDCDHR
                     ANASDACPADFDTMADADGDGVGDACDPDPAGAEGNHIAQFDGFDNNLGGWGITSGRW
                     ELGGGVFKQPETADTRVEKTVSMKFPSVEIIVLDLSTVGNGSVSAFGLAGASDVRCSV
                     FKRESDGVETLEIGGVLLPGKQVALSGTGPLRIMGGQRQDGMFYCTARHGTNVDAIVM
                     SGVAGPVTIDKVGFATSSASTTISSITLYDVPMM"
     gene            12759..13814
                     /locus_tag="VMZ53_31760"
     CDS             12759..13814
                     /locus_tag="VMZ53_31760"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HUS33136.1"
                     /translation="MNRLALFAVMVALAACGRGSNKAKLREPVGSAEPVVDDGGIVAL
                     PAAPPLPDVPVGLPPVPANPRITPEAVALGELLFYDTRLSPDGKRACALCHDPAHGFS
                     GRKSDAADGKANLRRTPPLVNLAWVKEYGWDGRFTSLDDFLASHVKGQLGDMLDGITT
                     RMTAVPTYAAHVARIGGTPRDALVQSLEAFVLTRYEGDAPWDRQERTALSRPGQAPND
                     PVLAGYQLFTGKAQCAQCHTPPLYTDGNYHQIEKNPYNDAARGRVEGHLQGAFKTPTL
                     RGAASRTDFFHAASKQSLDEVVAFYAESGAAATPDPADQDPLVRKIKLTADEQKQLVA
                     FLKALASDRVPAAPVLP"
     gene            13929..14534
                     /locus_tag="VMZ53_31765"
     CDS             13929..14534
                     /locus_tag="VMZ53_31765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33137.1"
                     /translation="MPLTCDFTGSYYDPAPWTERPCAAEYSVPEGCPIHFVTGAPMPF
                     DGWNLSAQVISPDGTGTLASSTTKSIGSDEQDFTVMDTLSCDCAWTTMHVSFQHMEVD
                     VPSAVAGDSVILWGAGVQSQFEIEITAAAPCPTPEWPTEYRAGLACDRCPEPVDEDDD
                     HWDGPGDFDVGCSAGGDPSLLLAGLVLLPFLRRRAKIVSSK"
     gene            14561..15031
                     /locus_tag="VMZ53_31770"
     CDS             14561..15031
                     /locus_tag="VMZ53_31770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33138.1"
                     /translation="MWWRRLRWFLTLAALCAIATCPAAKRSCTAKNRSREADELVTAI
                     ADRVAHNVAVTGKVPLMPAGPSPLMSCCEQDGGVCKADFAVWSSAGWRDLGFSIDGDY
                     RYIYQYVPDPSGKSAVIRAMGDLDCNGVTSLYELKLTVVGTLVHRRWRRVNPYE"
     gene            15044..15508
                     /locus_tag="VMZ53_31775"
     CDS             15044..15508
                     /locus_tag="VMZ53_31775"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUS33139.1"
                     /translation="MRMLLALLLIAACGKNDEPPPETTGAPNAKQESGPGTRRGAGPA
                     NATEQAHKMFETVCAMCHGTDGTGRGPAAENLNPKPRNYTDPAWQASVTDDEIRKTIL
                     LGGQATGKSASMPGQPQLKDQPEVLDGLVQIIRGFKQGDAPAAGSGAGSAAK"
     gene            15505..17091
                     /locus_tag="VMZ53_31780"
     CDS             15505..17091
                     /locus_tag="VMZ53_31780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33140.1"
                     /translation="MTSSLATSPDGQWAVAREGRELAVFANAAGAPIARLGLDSDDTD
                     VALVGPPTSLVLVSRDERRGYVTLYQPPDFQLAARLEIESPMRLAAITGPRLALVSPD
                     MKQLVIVRTAGKALASQKLEVTGPIEFVVGLERNQVMLGLLKKIEVWDAVSGRPLLRP
                     QFQFPPPPRVIGTASGHMWAMREGGDEIFVYRLSDGRPFRHYAGSQIREVVSNPASPV
                     LLLVTERGLVRLHCYAHSLQLVDGVPQPAGALALLALGEETFALGYTGGGDEPWRMSL
                     TGTTAPATTTTEAAAAATAAANAGQPVPKSGTTPRGPGWRDALVGLADRIAQGGELDV
                     PTIALDTELGELANRLKLTPLARRGLIILYALHLAGVRGLSIAKLAEAIGDWSEALGQ
                     GDLGALALVKTKHGNVRLRRAVTDALDGLAPRNVRLVGNGPSTAKPGAFRVGREGRAD
                     VDVEAELVRQLGRIAVVEGRLHAGLLEARLHGAAAVTTCVPDEKPLPWPRDASLVLVL
                     YGTASSWVADVPTLPSATAS"
     gene            complement(17078..17815)
                     /locus_tag="VMZ53_31785"
     CDS             complement(17078..17815)
                     /locus_tag="VMZ53_31785"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HUS33141.1"
                     /translation="MSREVDLYKDKIEVSLDGRQIFYLFFGGAVIVGLVFVLGVMVGR
                     RVEARGHVDRANVTTTADPLAALDRLEGGNRLSFQDALRGTETAPSDVEKKIESMQKA
                     PAAPVKSEKKAEPKKEEPKKDEPKKDEPKKVEAKAEAKVEEKKVEEKKVEEKKTEAKV
                     EEKKVEEKKQKFTLQLSSFQDKNEAQAYLDSVKGSGYQAYVTEGEVEGKQFFRVRMGS
                     YKSLEAANEAKSEFEKSAKKTAIVTKL"
     gene            complement(17884..21219)
                     /locus_tag="VMZ53_31790"
     CDS             complement(17884..21219)
                     /locus_tag="VMZ53_31790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33142.1"
                     /translation="MKRALVLSLVLAACSDATNNAATQLNLDRPVDVAFACYGGLRLN
                     TDSSVVASAQAIASCDIRSAERAADAPKPIPPGQEDRPGQPVPSVSWYAFILQSGPGT
                     VAVARFEAKPASTFSAGDVAIQDADPLTPGKNSISVGEDPIAIATDKIGCKEVIANAG
                     SCDLSILDVNKAIAGVYTPTDREASVSRLEVKNAAGAVIRAKPAAMAFEPAGGNIGDA
                     CPSTATGIAYIAYPGCHLVAGVDVGSGTMVTAISFETGVPVVVTDTSAITCPAECDGT
                     MAPTPGLRPVTVDLERDERTNRSVLAIGSENSNAVAIFDLDSSNFRPLSQIPVPPLQN
                     TNGRLGITSLAISPVIGMGGTTGIIDDSAQSSQHQFIYAVANDSTVRVVDIGDPTGDV
                     PVECDTQVDPRYIKDIRDVDRLACFPNQKFETSPATCTTNTDCGSDKFCLVDPSATPA
                     GTKRCTLTPPRRPGARGPGIELIGDAVPTSVDFFRADSQGVTNDVLRLIGHFAMITAA
                     AGQTYVVNVDNDDYPDFVSPDVGKSDRLRTQIPLVIAHQLRDAVPDRGKIAEEASEVM
                     GAPATPVCDDPGPDPDSNAGNSGGARAAANPSRTVPAGVLAPEKYGGLPSIRQVLCES
                     PKGNAPVSELSFSAPIPVREAVFPDLRGLRADEVWTLTWEGSLSLDKVDAAIDGPSIR
                     YSQTLVDGGGMRLVDQTRPFCDAGVEPYDIVQLRGCDPAIGDAGCPVGYTCYVHPQSQ
                     VAGLGACMLTDEAERLANACRDFLVSLRRYTVGRTKSGELQILPRKHELRTTPLDGCV
                     DDAQCHDLANYAIHNVNSANPKDDTTSEDAHTYYCRVDNDRKPKAGTGKRCLMACMSD
                     TDCATGTICRANAEAAPSMGYCMEGVVPPQSCVNAPQRYELRAGEAFSVVGSRQGFLH
                     SIISDTSGNCIRDPNANPFMVGRLPLTAPACDPTADPLTGRLPNGTFEPNPCKASVDE
                     TEYMLNYIPNTCSLNNPDENLITRMAEAIRFRNRAVNLTVVDPTYPGDAQCHGDRQGG
                     RTNVPLVSPGFQMAFRITGGFAPLPLSISPSYPVKVLRGPQQSIWVVDEGDFLSTSVT
                     TPSTRGKVFRVESRAIGVVNLVD"
     gene            complement(21216..22556)
                     /locus_tag="VMZ53_31795"
     CDS             complement(21216..22556)
                     /locus_tag="VMZ53_31795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HUS33143.1"
                     /translation="MLLLGAAAGVGCTASAEEVRPPERELFFPTGAAISPDGKFLFVS
                     NANSELRYDSGSVSVIDLDYVDRVADGWVGMQSQEAGCSIDPTHKETLACGEEPFIVE
                     HANARIGNFATDIAVQDTGNGTARLIIPTRGDPSITWIDWNGTNLTCGGGQGFSQCDE
                     THRLSYVHDDPDLDSAPDEPFDAFADSNGQFAVVTHLTTGAVTLIDSPIGGTAAIADV
                     IGKETPIFAADPLTGLRGATGVAARPSAGGDIIYVASRSEDRVQMMTVGHPANEGGGG
                     GARVYPYLVPSNYFFLNLVGANAGGSTDSRGLTFSPGGDRLYLLNRRPPSLQVYDTSL
                     NDAGFPRNMGLGATDICRQASTLTVLDAGDGERAYVTCFQDGQVYVVDPRGLTSVEDI
                     VLVGRGPYTTVGSAAHKKVYVTNFLEDTIAVIDVSPTSPRKNRVVLRIGDVRPL"
     gene            complement(22631..25285)
                     /gene="ppdK"
                     /locus_tag="VMZ53_31800"
     CDS             complement(22631..25285)
                     /gene="ppdK"
                     /locus_tag="VMZ53_31800"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HUS33144.1"
                     /translation="MKYVRQFGGGSAEGRSKDKALLGGKGANLAEMASLGLPVPPGFT
                     ITTEVCRHVMEHGVDKYPEGLDAEVNEALSKVEQLTKATFGDSTTPLLVSVRSGAPAS
                     MPGMMDTILNLGLNDTTVAALAALSGNARFAWDCYRRFVAMYGEVVLGVVPATESDEP
                     PFDKILDEIKSKHRANRDQDLKEDALREAVAAFKAKVKEATGSPFPDDVRAQLWGAIG
                     AVFRSWNNPRADVYRKMQGIPASMGTAVNVQAMVFGNLGDDCATGVAFTRNPATGTAE
                     LYGEFLTNAQGEDVVAGIRTPEPIGELAKKLPAAHAELVRVAKLLEDHFRDMQDLEFT
                     IQKGQLFMLQTRNGKRTGKAMVKVAVDLVAEGKLTPKEAVLRIDPQKLDEVLHPTIDP
                     KARPTPIAKGLPASPGAAIGRVVFTANAAEEWHLRGEQVLLVRTETSPEDIHGMKAAA
                     GTLTARGGMTSHAAVVARGMGKCCITACTSLRVDAAKKTATFAGGGKEHKLKEGDTLT
                     LDGSTGEVFLGSAPLVPAQLGTELGTLMQWVDQYRRLKVRTNADTPTDARTARSFGAE
                     GIGLCRTEHMFFQPERILAVREMILANDEGGRRAALAKILPMQRGDFAELFRVMDGLP
                     CTIRLLDPPLHEFLPHTQAEIEAVAAELGQPANKVAAKVAELTEANPMLGHRGCRLAI
                     TYPEIYEIQVQAIGEAAALVAKEGVSVIPEIMIPIVMVPEELRRLRVLTANAMNAALG
                     GAAIPYTIGTMIELPRACVVADRIAEHADFFSFGTNDLTQTTFGLSRDDAGRFLPTYV
                     DSGALPADPFAVLDPDGVGALIELGVRGGRATKPGLKVGICGEHGGEPSSVEFCHRAG
                     FDYVSCSPFRVPIARVAAARAALLGDKP"
     gene            complement(25282..>26944)
                     /gene="glyS"
                     /locus_tag="VMZ53_31805"
     CDS             complement(25282..>26944)
                     /gene="glyS"
                     /locus_tag="VMZ53_31805"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HUS33145.1"
                     /translation="VVTGIPWPKGMRWGWSETAFVRPVQWLVALFGGEVIPLTWAGQT
                     SGRTSRGHRFLSPGPIEIASADKYVETLRAANVVVDQEARKTEILAELSRLERETGCK
                     VRKDDALVDEVVNLGEYPRGVSGQFDPSFLEVPHEIIVTAMRTHQRYFAMETPDGKLA
                     PRFATMMATIVKDPAVVARGNETVLASRLSDAKFFFSEDKKKTFDDWNKRLDGVVFQA
                     KLGDKAKTIGNKVERLVEIVSALGGSATAKKAAQYAKSDLASHAVGEFPEVQGTMGKH
                     YARIAGFGDDVAAAIEQHWWPKGQGAELPKSDDAALIAIADRMDTIVGCFAVGLEPSG
                     SADPFGLRRAAIGIWQILLERGWTDKFGDARAAAEKALAAQGVKLKDGKPLDEFFKAR
                     LRGIFIESGIPPQDADAALAQNASDPVDARARALACAKISKEAREVFKRVANILDDAR
                     AKKLAIGTSPDASKFVSPVETNLHAAIATAQAAEKAARDKRDYAAVFESLEKLRPTVA
                     AFFDKGGVMVMDPNAELRDNRLALLNWFISPYLAIADFRLLAGAA"
BASE COUNT         4359 a         9012 c         9009 g         4564 t
ORIGIN      
        1 gcatgcgcag cccgccgatg tgccaggcct catcaagctg atcgagaacc agccgagcga
       61 catggatcgc tcgacgtgga aagagaagcg acgcgacgcc gcgcgcaagc tcggccagag
      121 caaggacaag cgcgcgacgc cggtcctcgt gcgtctcgcc gagaccgaga cgttcgacat
      181 catcggcgac atcgcgatcg aaggcctcgg ctatctcaac gatcagtccg cggtgccggc
      241 gctgcagaag gtgctcgccg atacgtcgcg cgacaagagc tcgcgtgacc tcgcgaagaa
      301 atcgctcggc aagctcggcg cgccggacaa accggtgggc ggcaccacgg ggacgaccgg
      361 tacaacgggc acgacgggaa cgacgggaac caccggtccg acgggcggca cgacaggtac
      421 gacgggaacg accggcacga cgggcgccac cggcagaggt accggcaaga cgggcggcac
      481 cggcggaacc accggctcga cgggcaccgg aaaaactggc ggaaccggcg gaaccggcgg
      541 aaccggcacc ggtggatcga tcaccggcgg aacaaccggc accggcacca cgggtggaac
      601 cactagcggc ggaacagcga ccggcacgac caccaccgtc ggcagctcgg gcaccacgac
      661 cggcacgggc tcgatcacga ctggcatcga gggcggtggc gaaacgggcg gctcgacggg
      721 cggcggtgac ctcacggtgg gtctcggcgg aggcaacgca agcgccgtcg aaggcatgcc
      781 cgagatgaac gacgacgtga tcgccgcatc cgagcgcctc acgttcgcgg caggcacggc
      841 gaacctccag atccaaacga cgccgaacga caacaacggc aaccgcaaca agcacatcaa
      901 cttcgacacc gacgtcgcgt cgaattatca gcgccgcatg gatgccgaat cgcgggcgtt
      961 cgcgctcgat ggcgacgtga agctcgtcgg tgcgttcaac tcacagcaag ggcgaggctc
     1021 gtcgaagggc gtcttgttca acacgcgcat cggcggcgaa gcgcgcttct actcgaacaa
     1081 gctctacggc atcggcaagg cagctggcgc actctcgatc cagtacatca actatcgcga
     1141 tccgaacgac aacaccggcg gcaacgacct caaggacacg acgtttgccg tggatctcca
     1201 gatcgcgctc ggcggtggct tcggccgcgt cgtcgatatc ggctcgcaga tccgcgtgca
     1261 ccggctctcg cgcatcctcg atgactcgcg cgcgctcggc aaaccgatcg acgccgccac
     1321 ctcgaagaag ctgcagctga cgtggtgggc gctgcgtaaa gaacgcagcc agttccgttc
     1381 gctgatcgcg acgatcgcag tgctgcggga agccggcatc ctgctcggcg aaccgaacgc
     1441 cgtcatcacg tatcagatcc tcagcgtgct ccaagacacg agcctgtttt tgcgtccgag
     1501 cggcttcgac gtgcagctcg tcgttggcga gggctacctc aagcggccga ccgacgacgc
     1561 gggcaactgc ctcgtgacct gcggacgtct cgagcaagtg atcgcgagcg cggcgtacgc
     1621 gcagcaagtc gacgacgaca agctcgagat ttcgggcggc ggctatgcgc gctatcgcct
     1681 gttcgcgccc gacgacggca cgccgacacc gtgggcgatc ggtgcgagcg caggtatgcg
     1741 gcgcttcacg tacggccagc acggcgatgt gttcggcgtg ttcgatctgc gcggcgggct
     1801 cgccttgtcg agcgacggca gcgccgatcc gaacaatccg agcgacaagg cgcttcgcat
     1861 cgaaggctcg ctcggcttca cgtacgtgct caaccaagca tccggcattc gcctcgcggc
     1921 ggaggtcgcc gaagaccagg gcacgatcta tctcggcgcg tcgctgcagg cgacctacgg
     1981 cctcctcgac ggaacgtacg cgcgctaact cgtcgggatc aggcccacaa atcctgggtt
     2041 gaatggtcgg accaattgct ctaaggtttg tttgtgtccg acctgagcgc ccagcagcac
     2101 gagaccttgt tggccgtcgc cgagacggcg ttgcctgcgg gccgcttcat cccgggcgcg
     2161 aacgcggcca ccgtcgagaa ggtcgagcgc ttcgtcgaga agctgccctc gcagctatcg
     2221 tttgggctca aaggattgct ccgcgggctc gatgcagcgt cgtggctcgg tgagcggcgc
     2281 ccgttccgca aggtgaagcc cgcgaagcgg ctcgcgctcc tcgagagctg gcggcaaggc
     2341 gatccgattc gccggctgat gctgcgcgcg ctcgtgagcc cgctcaaggt cgcccacttc
     2401 gacgaccctg ccctctacaa gcagctcgga tgcgtctacg acgtcgagaa ggcgaaggcc
     2461 gaggccaagc ctgcgtacat gcgcgatcgc gtgcaccgcg ccgacgagct cgatggcgac
     2521 ctcgcgatcg aatgcgatgt ggtcgtcgtc ggcacgggcg caggtggcgc cgtcgtcggt
     2581 cgcgagctcg ccgaggcagg cctcgctgtc gtattcgtcg aagagggtca ctactacgag
     2641 cgcacgcagt tcaatggccg cgcgatcgac atgcagcagc gcctctatcg gcgcgccggt
     2701 tcgacgttct ccgtcggcaa cgtcggcatt ccgattccgc tcggccagtc cgtcggcggc
     2761 accacgacga tcaactcggg cacctgcttt cgcacgcccg atcgcatcct gcatcactgg
     2821 cagaaggacc tcggcctcga cgagctcggc cccgagcaca tgggtccgta cttcgatcgc
     2881 gtcgaggcgg tgctcggcgt cgagcctgcg cgcccggagc tactcggcgg caacggtcgc
     2941 gtgatccaac gcggctgcga cgcgctcggc ttcacgcgcc acggcccgct gaagcgcaac
     3001 gcgcccgcct gcgacggcca aggcgtgtgc tgctttggct gcccgaccga tgcgaagcga
     3061 tcgacgaacg tctcgtatat cccgctcgca ttgcgcgccg gcgctgagct gttcgccggc
     3121 gccaagatga cgcgcgtgat cgtagatggc ggtcgcgcgc gcggcatcgt cgcgacgacg
     3181 accaagggcc acgtgctcac cgttcgctcg cgtgccgttg tcgtcgcgtg cggctcgatc
     3241 atgacgccca tcgtcctcgg cgcgcagggc ctcggtggcc gctcgggcca gctcggcaag
     3301 aacctgtcga ttcatcctgc gtgcggcgcg ctcgccgagt tcgacgagca gatcatgccg
     3361 tggaagggca tcccgcaggg ctactcgatc gaagagctcc acgaggaagg catcctgttc
     3421 gagggcgcga tggtgccgct cgagatgacg atgaacatga cgcagatgat cgggcctgac
     3481 ctgatccggc tcgccgagag cttcgaccgc gtcgcgagct ttggcttcat ggtcgaggac
     3541 acatcgcgcg gctcggttcg cgaagtgatg ggccagcccg tgatccaata ctggctgcaa
     3601 gacaaggacg tctcgcacat caagcgcggc gtcgacgtgc tctcgcagat cttcttcgcc
     3661 gccggcgcgc gacgcgtgca caccccgatc gcgggcttcg acgtgctcga gtccgaggcg
     3721 gacctcgtga acctgcgccg cgcgaacatc cgcgcgtggg acctcgacct gtccgcgtac
     3781 cacccgctcg gaacggcgcg catgggcgcg gacccggcga gctcggtcat caacgcgaac
     3841 cacgagatgc acgacacgca gggcctcttc atcgtcgacg gcgccgcggt gcccaccgcc
     3901 ctcggcgtga acccgcagat cacgatcatg gcgctggcga cgcgggccgc agaaaaaatc
     3961 gccgcgcagc tgacctgatc tcaggttttc gagagtcggc cgcgagcttt cgcgacgttg
     4021 cgcaggcacg gcacgtgctg tgcggatcga tcgatgagga ctgctctcgc cgccgcttcc
     4081 accctcgccg ctgtcgccct gtcctgcctg cccggcctgg ctgaagcccg accgttcacc
     4141 gctggcgttg gtgtcggccg ctcccaagcc aaagccgatg ccgaaggtga tgcgagcgac
     4201 acgctccagc tctttggtcg cttctcgttc acgaagcgtc tctccgcgca gctcgagatc
     4261 cagaagctgc agctgcccta cggcgacgtc accgttcgta gcggcacgct cctgctcgtc
     4321 gtcgaccttg gccagcgcgg ccacctcgtg ccgatcttga tggcgggctt tggaggcgcg
     4381 tccgcgacgg acgactacgg ctacgagacg agcgcgtcgc ataccgaagg cggcgctggc
     4441 ctcgagtacc gcgccgatgg cgggctcacg atcggcgtcg acctccgcat cggcggtcgc
     4501 tcgctcgacg acgacgacgt gaagatccag ccggcgacga cggacgtcat cgcgatctat
     4561 ccgtacaact cgctgcaagc cggcgagtac cgcagcgcgc gtctctacgc agcggttcgc
     4621 ttttagagat cgatcttgtc gcggaacgtc gcggatgcgc cgggcttgaa ccggacgtcg
     4681 gcgaggacgt cgtggacgca gctcgcagtc gacgacgaca ggtccgtgtc gatgatgttg
     4741 aggaagccga cggcgcccgt ctcgtcgatc gcgatctcga cgtggacgta cgaagcgaga
     4801 cccgcttttg tgatcgcgcg cgtgcactgc gcgagccgtc gctgatcgat gcccgcgtgg
     4861 agctggcggc ggcgctcggc ggtgccaccc gtcggcgcgg catcgggctg cacgacgagg
     4921 cgcgcgggct tggccgcggc gatgtcgacc cagccagctc gccggaagcg ccccgcggca
     4981 tcggccgtct cgacggtgtg gcgacctggc atcacgcgca cgagcagcgg tgcatcgccg
     5041 agctcgacgc catcgacgcg cacggctcgc ttgctcgtcg tgctgatctc gagtggcgcg
     5101 ctcgtgcgga cgagcgagtc agcgtcggtc cagatcggcg tcattgccgg cgtcgcttgt
     5161 gcgagctgat cgagctcgtc gacggtgagc gcgcgcacgt gctgcggatc gagtggcgcg
     5221 tcttgcgtcg tgacgagctt gcgcgcggtc gcgacttccg ccgtgccgtg cgagtcggtg
     5281 acgatgacct tgccgtggcg gcacgcgacc tgcgtcgacg cgccgtcgtg cacgacgcgg
     5341 aattgcgtcc cgcggacttc gacggtttgg tcgcctgcga tcacgacgaa gcgctggccg
     5401 gcgacgcgag gcgcgacggt gatgtcgacg gtgccttcga cctttagctc gatgcgctcg
     5461 gcatcgaagc ggtgaagctc gagggtggag cgaggtccga gagcgaacgc gctgtccgcg
     5521 ccgaactgga cgtcgatgcg gccgtcgccc gtcgcgatca cgttgcccac cgcgagcgtg
     5581 cgttcgaagg catcggcgcg gcgaacgccg tcgatcatga cttgccccgc gagtctgctg
     5641 acgacgccag tgaccggtgc gggcggcgag acgagcgtga cggcaatcgg cgcttgatcg
     5701 ttcgtcgcga cgggctgctt cggcgtgctc gatcccgagc cggtgacgag cacgagcgtg
     5761 agtgcggcag cgccgaggat gcctgcgcca gcaagcggcc agatcgcgcg gtgacgttcg
     5821 cgtggtgcgc ggtcgcgagc gcgcttctcc gacgagaccg accagtgaat gcgtgcgcga
     5881 accgtgtccc atgcgacgtc gggcgcctgc tgcgcgcgca atgcggggaa gctctgcgat
     5941 gcggactcga cgcgatcgcg cgcggtcgca caacgcttgc aggcgtcggc gtgcgcttcc
     6001 atctgcgcga cgacggatgt ggcgagcttg ccggcgaagg catcggccca tttctcgggg
     6061 cggacgtgac tcattcgtca cctccgtcat ccgccggttc gccggcgagc actttgtaga
     6121 actcgcgacg cgcgtagaac agccgggtgc gcacggtcac cggattcgaa tcgaccagat
     6181 acgcgatctc tttgaggtcc aggccctcga tttcgtgcag gtacagaacg acgcgcttct
     6241 tcggtgcgag cgagtcgagt gcgcgataga cgcgctcttg atcctcgcgg cgctgcagcg
     6301 atcgctccgg cgtctcgggc cgctcggtca agctcgggtc gacggccgcg ctgtcgagat
     6361 cgaccatccc gatcgccgca gggcgacgac ggcgagtgcg aatgcggccg agcgcaacgt
     6421 tgacgcagat ccggtagagc cacgtcgaga ggcgcgcgtc gccgcggaag cggtcgaggc
     6481 cgcggaacgc gatgacgaag acttcctgga cgaggtcatc gaggtcggtg cgatcgccga
     6541 ggacgcgata gaggttgccg gcgacctggc ggcgaaaccg gtgatagagc tcgtcctgcg
     6601 cggcgcgatc gtcacggcgg cagcgctcga tcagatcggc ttcatcggcc ggatcgaggg
     6661 cggctgcgga gccggtttcg cgcttcacga tcgcgagcat aacgtgtcga gacgttagtg
     6721 cccgggaggg taccgtgcgt tcacgcccgc cctgtgatgc cgcctacacc ggcctaccgg
     6781 cgggtcggaa gaaccgtgaa gtcgagatcg cggtcgagca cgcaatgcag cgcgtcctcg
     6841 tccttcttcg cctcgaagtc acacggaaac tcgtccttcg cggtcgtctt cgcgcccttc
     6901 gtcgggacgg tcatgacgac cccgtcgcct tcgattcgcc acgtgccctt cttagagatc
     6961 gagaagccct cgccctggca catcgtcttc acggtgacct tgaacgcgcg gtcgcgcgag
     7021 acggtcaggt ggacctcggg ggcctcgcag ctcgtcatgc tctgctccca ctggccgccg
     7081 aagcccatga ggcgctcgta cggcgtggtc ggcttgcggt cgaggccaac cgccgcgcgg
     7141 tatttcggat cagcgcgcac cgccgcgaat gctttttcaa agcgcgcttc gacgagccac
     7201 tcgatcgcat cgctgcgcga gctcttcgcc agcttgtcga gcgtcgcgat cgcatcgccg
     7261 tttgccttgt tcgcgatctg cgcggtcgcg agccggtaca tcgcttcgct gtgatcgggc
     7321 gccatcgcga ggacgccttg ccacgcagcg acagcctttg ccttcggcgc cttcttcgcg
     7381 gcggcgatcg ccttggtgac tttcggatcg tcggcgggtg cagccgtggt cgtcgtcgtc
     7441 gtggtcgttg tcgtcgtcgt ggtatccggc ggcttgatcg gctcggtctt gccggttgtc
     7501 ttcacgagct cgaacgcgac gacgctcgtg acttccttgc cgagcacgcg ctgcgtgtat
     7561 gcgaccgcga cgcggtcttc gtactggctc gagtagacct cgacgacctt gccgagcggg
     7621 tcgacggccg tccacgtgac gaccgcggtg ccgtctttcg tgacggtgag gttcttgccg
     7681 gagatggtcg cggcgaatac cgccttgggt ccgcgctgga tgatcggctc tttgatcgag
     7741 agctgcgcga cgacgtccgc ggtcgccttc tcgcaggtcg gcgtgccctt cgcggcgcct
     7801 ttgtcgacgg cgagcggctg gcacacccgg tcttgattca cgaggaacgg cttgtcgacg
     7861 ccggagaagc ctccgaacgt gtcggcggca gcgggcgtcg ccaccaataa gcccacgagc
     7921 ggcaaagcgg atcgcatcgc cattacgacg ctatagcgcg attttcattc agtcgcccgc
     7981 ctttgcgaat tgaggtacgg tcaacaggtg cgttccatgt tcacccgcgc aagcgcggct
     8041 atcgaccgag gcgtggttcg cttcatggaa cgacgcatgg cgccaagcgg accccgaatc
     8101 gacccggggg atgcccgcac gcggctgatc gagctcgcag accactacag cgacgggacg
     8161 ctcggccatc cgagcccgtt ctttcccgag cccgatgtgc ccgacatcaa gatggcgcat
     8221 cagggcgacg gtccgctcgg gacgcaggtc gtcgaccttt cgttcgcgtc gtcgtatcgg
     8281 ccgtatcacc cgctcgcgcg cgatccatat ctcgccgtga ccgagaacat gacggctcac
     8341 gctcggtggt ggacgagcga tcgcggccga ccgacgatcg tcctcattca cggctggggc
     8401 gccggaagtc actggatgac ggagcgcgcg ttccagctgc cgtactggct gcggcacggg
     8461 tacgacgtgc tcgcgttcca gctgccgttt cacggcaatc gcgcacctgc gggcatcggc
     8521 aagagcggcg cgctgtttcc gtctgcaaac ccgctgcgca cgaacgaagg cttcggtcac
     8581 gcgatctacg atctgcgcgc gctcgcccga ttcattcgct cgcgtggcgc gtcggccgtc
     8641 ggcgtgatgg gcatgagcct cggcggctac acgaccgcgc tgtggacgac catcgccggg
     8701 cccggtgacg tcggcggcgt cgacttcgcg gtcgcgatga ttcccgcggt ctcgatgtcg
     8761 cggctgttct ggcagcatgg cgaagattcg ccggtgcgtc gtcgcgcgtc gaaggccggc
     8821 atcaccgagg atctgctggc cgaggcattc gcggttcacg ctccgaccac gcgcgcgccg
     8881 cggctgccga aagatcgcct atacgtgatc gcgggccgcg gcgatcgcat cacgccgccg
     8941 gatcaagccg aggcgctcgc ccgtcactgg gatcgcgaga tcacctggtt cgacggcggt
     9001 catctgacac aggtgggccg tggcgatgcg ctgcggaccg tgcgacgttc gctcggcgcg
     9061 cacgcgtttc ccggacaagc cttccgtggc tgatacatcg aagcctctcg ccgaagttct
     9121 cgcgaccggt ccactcctcc tcgacggagc gatgggctcg ctgctctacg agcgcggcgt
     9181 cctgcacacg cgcagctacg acgagctgaa tctctcgaac cccgagctga tccggacggt
     9241 ccaccacgat tacgtgcagg ccggcgcgga gatcatcgag acgaacacgt tcggggcaaa
     9301 ccgcatggca ctcgcgcggc acggcctcgt cgagcaggga caggcgatca accgcgctgg
     9361 cgtcgagctc gcacgcaagg cggcgggtga tcgcgcgtat gtcgccggcg cggtcggccc
     9421 gacgggcgtg aagtttcaca tcgcgacggc cgcagagcgc aggctcgcga ggttcgcgct
     9481 cgccgagcag atcgacacgc tcgtgctggc cggtgccgac gcgatcatcc tcgaaacgtt
     9541 cacgtcgatc ctcgagatgg aagaggcgat ccgcgtctgc aaagagcgcg gccccaaggt
     9601 tccggtgctc gcgatgatgg tgttcgacgg tcagtcgcgc agcgacggcg gcctcggccc
     9661 cgcggagatc gcggatcgca tgatcgcggc gggcgctgac gtcgtcggcg gaaactgcgg
     9721 catcggtccg gcggagctct accaagtcgt cacgaacatg gtcggtcgcg gcaagcccgt
     9781 catcgcgcag cccaatgccg gtctgcccgc atcggtcgaa ggccgcacgc tctacgtcgc
     9841 gaatcccgag cacttcggtg tgttcgcacg ccgcatgctg aagagcggtg tccgcctcgt
     9901 cggtggctgc tgcggcacga cgccggagca cacgcgcgcg atgctcggcg cggtccggat
     9961 gctgcgcggc gagaaggtcg acgagctcgg cacgaaatca tcgccgacga tccagatctc
    10021 accggggccc gcacccgcgg cgaagatcgc cctgcccgat cgctcgcgca tgggtggccg
    10081 catcgcgcgc ggcgagttcg tcgtctccgt cgagctgacc gcaccgaccg gtctcgacat
    10141 gacgaagacg aagcagcagg tcacgcagct gatcgaaggc ggcgtcgaca tcgtcaacat
    10201 cgccgacggt ccgcgcgcat cggctcgcat ggccaacgtc gcggtgtgcg cgcgactcgc
    10261 gagcgagacg aacgtcgagc cgatcctgca cgtgtgcgcg cgcgatcgca gcttcctcgg
    10321 cctcgtcgcg cacctgctcg gcgcgcacgg cctcggcctt cgcaacctcg tcatcatcac
    10381 gggcgacccg ccgaagatgg gcgactaccc gttctcgacg ccggtctatg acgtcgactc
    10441 cgtcggcctg ctgcgcatgg cggcgggtct caatgccggc gtcgatccag ccggcaagga
    10501 atgcgagccg acgcacttca cgctcgcgac gggcgccgag cccgctgctc acgaccgcga
    10561 acgcgagcta cgccggctcg aagagaagaa gctcgccggc gccgagctcg tgatgacgca
    10621 gcccgtgtac gacccgcgca cgctcgagcg attcctcgac gacgccgcgc cgctcggcct
    10681 gcccgtgatg gtcggcatcc tgccgctcgc gtcgcaccgc aacgcggagt tcctccacaa
    10741 cgaggttccc ggcatgagca tcccggccga gtaccgagag cgcatggcga aagtcggcgg
    10801 cgggccagcc gcgcgcgtcg agggcatcaa gatcgcgcaa gaggcgctcg gtgcggtgaa
    10861 gaatcgcgtc gcgggcgcgt atgtaatgcc gccgttcaat cgcgtcgagt ccgcactcac
    10921 ggtgctcgag gtcgctcgcg atcgctggaa acctggagcc cgatgagcct gacgctcgac
    10981 gaagcgcgcg cactccacga cgaagtctgc gtgatcgatc tccacgccga cacgccgaag
    11041 ctcatggaca agctcggcta cgacttcatg gcgcgccatg acacgcccat gccgcggagg
    11101 gcgaacatcc tcggccacgt cgacgtgccg cgcctgcgcg aaggcgggat ggccgcacag
    11161 ttcctcgcgc tgtggacgtg gccgaagtac gtgcccggcg ccgagcgcag caacatccgc
    11221 tcggtgctca accagctcga cgcggtgcag tccgtcgtcg acaagaatcc cgagcagctc
    11281 gcgtggacgc gcaccggcgc agagatccgc gcggcgaagg ctgccggcaa gatcgcagtg
    11341 ctcggcggca tcgaaggcgg ccacgcactc gagggcaagc tggaagcgat cgagatgttc
    11401 gcgcggcgcg gcgtgcgcta tctcgggccg attcatctct ggccgtcggc atacggcagc
    11461 tcgtcgaagc agccgaagaa ggacgatggc cttaccgcac tcggcatcga cgtgattcgc
    11521 gagtgcgagc gcaccggcgt cgtcgtcgat ctctcgcaca tgagtcacaa gtcgtacgcg
    11581 caagcgctcg aggtcgtgaa gctgccgccg atggtctcgc acaccggcgt cctcggcgtg
    11641 cacgagcact ggcgcaacat caccgacgag cagatccgcg aggtcgcgaa tcgcggcggc
    11701 tgcatcgggg tgatcttcgc gcgcaattat ctcggcggaa aatcgatcga ctcggtcgtc
    11761 gatcacatcc tccacgtgat caacgtcgcg ggcgaagacg tgccggcgct cggctccgac
    11821 ttcgacggct tcgtcgtgcc gcccgaaggc ctcgaagaca tcgctgcgat gccgaacctg
    11881 acggtcgcgc tctcgcgccg cggcgtcgca ccacgcgtgc tcgagaagat cctcggtggc
    11941 aacgtgctac gtgtcctcga tgcgattccg gcatggggca agctcgctgc gtgaaggcta
    12001 ttgtcgcgct gctgctcgcg tgtgccggct gcgacgcggt gttcagcgtc gaccacgtcc
    12061 gtgagcagct gacgcccgac gcagatgtct gcgcgcaggg cgacgaagat tgcgaccacc
    12121 gcgcgaacgc gagcgacgcg tgccccgccg acttcgacac gatggccgac gcggatggcg
    12181 atggcgtcgg tgacgcgtgc gatccggatc cggcgggcgc cgagggaaat cacatcgcgc
    12241 agttcgatgg cttcgacaac aacttgggcg gctggggaat cacgagcggc agatgggagc
    12301 tcggcggcgg cgtattcaag caaccagaga ccgcggatac gcgcgtggaa aagacggtga
    12361 gcatgaagtt cccgtccgtc gagatcatcg tcttggacct ctcgacagtc ggcaacgggt
    12421 cggtgtctgc gttcggcctc gccggcgctt cggacgttcg ctgttcggtc ttcaagcgag
    12481 agtccgacgg cgtcgagacg ctcgagatcg gcggcgtcct cctgcctggc aagcaagtcg
    12541 cgctcagcgg aacgggccca ctgaggatca tgggcgggca gcgccaggac gggatgttct
    12601 actgcaccgc gcgtcacggc acgaacgtcg atgccatcgt gatgagcggc gtcgccggtc
    12661 ccgtgacgat cgacaaggtt ggattcgcga cgagctctgc gtcgacgaca atctcgtcga
    12721 tcacgttgta cgacgtcccc atgatgtaag ctgcgccggt gaatcgcctc gcgctgttcg
    12781 ctgtgatggt cgcgcttgcc gcgtgtgggc gtgggagcaa caaggccaag ctgcgcgagc
    12841 ccgttggttc tgccgagccg gttgtcgatg atggcggaat cgtcgcgctg cccgccgcgc
    12901 cgcctcttcc cgacgttcct gtgggattgc cgccggtgcc tgcgaatccg cgcatcacgc
    12961 ccgaggcggt cgcgctcggc gagctcttgt tctacgacac gcggctctcg cccgacggca
    13021 aacgcgcatg cgcgctctgc cacgatcccg cgcacggttt ctctggtcgc aagagcgacg
    13081 ctgcggacgg caaggcgaac cttcggcgca cgcccccgct cgtcaatctc gcgtgggtca
    13141 aggagtacgg ctgggatggc cgcttcacga gcctcgacga ttttctcgcg tcgcatgtaa
    13201 agggccagct cggcgacatg ctcgacggga tcacgacgcg catgacggcg gtgccaacgt
    13261 atgcagcgca cgtcgctcgc attggcggca ctccgcgcga tgcgctcgtc caatcgctcg
    13321 aagcgttcgt gctcacgcgc tacgaaggcg acgcgccatg ggatcggcaa gaacgcacgg
    13381 cgctctcgcg tccggggcag gcgccgaacg atccggtgct cgcgggctat cagctgttca
    13441 ccggcaaagc gcaatgcgcc cagtgccaca cgccgccgct ctacaccgac ggcaattatc
    13501 accagatcga aaagaatccg tacaacgatg ccgcgcgcgg tcgcgtcgaa ggccacctgc
    13561 agggtgcgtt caagacgccg acgctgcgag gcgccgcatc gcgaaccgat ttctttcacg
    13621 ccgcgagcaa gcagtcgctc gatgaggtcg tcgcgttcta cgcagagtcg ggtgccgcgg
    13681 cgacgcccga tcccgcggac caagatccgc tcgttcggaa gatcaagctg acggcggacg
    13741 agcagaaaca gctcgtcgca ttcctgaagg cgctggcgtc ggaccgcgtg ccggcagccc
    13801 cggttttgcc gtagagcccg agggctgaca tcgctgtcgg ggacgccacg tcgaacggca
    13861 ttccgcggcg tgtaagtggc atggatcgca cgaggcgccc tggccctgcc tctgcagttc
    13921 gccgcggcat gccgctcacc tgcgacttca ccggcagcta ctacgatcca gcgccgtgga
    13981 cggagcggcc gtgcgccgcc gagtactccg tccccgaagg ctgcccgatc cacttcgtga
    14041 ccggcgcgcc gatgccgttc gatggctgga acctgagcgc gcaggtcatc tcgccggatg
    14101 gcaccggaac gttggcgtcg agcacgacga agagcatcgg gtcggacgaa caagacttca
    14161 ctgtgatgga cacgctcagc tgcgattgcg cgtggaccac gatgcacgtc tcgtttcagc
    14221 acatggaggt cgacgttccg tccgccgtcg cgggtgactc cgtgatcctg tggggcgcgg
    14281 gtgtgcagag ccagttcgaa atcgagatca cggcggccgc gccctgcccc acgcccgagt
    14341 ggccgacgga gtaccgcgca ggtctggcgt gcgatcgctg tcccgagccc gtcgacgaag
    14401 acgacgacca ctgggacggt cccggcgact tcgacgtcgg ctgttcggcc ggcggcgatc
    14461 cgtcgctgct gctcgcggga ttggtgctcc tgccgttcct gcggcgccgc gccaagattg
    14521 tgtcgtcgaa gtagcttcga tccggtagag tccgcgcgcc atgtggtggc gccgacttcg
    14581 ctggtttctg acgctcgccg cgctctgcgc gatcgcgacg tgtcccgcgg cgaagcgctc
    14641 gtgcaccgcg aagaatcgct cgcgcgaagc cgatgagctc gtcaccgcga tcgcagatcg
    14701 cgtcgcgcac aacgtcgccg taacgggcaa ggtcccgctc atgcccgccg ggccgtcgcc
    14761 gctgatgtcg tgctgcgagc aggacggcgg cgtctgcaaa gcggacttcg cggtgtggag
    14821 ctcggccggc tggcgtgacc tcgggttctc gatcgacggc gactatcgct acatctatca
    14881 atacgtgccg gatccgagcg ggaagtcggc cgtgatccgg gcgatgggcg acctcgactg
    14941 caatggcgtg acgtcgctgt acgagctgaa gctgacggtc gtgggtacgt tggtccaccg
    15001 ccggtggcgt cgcgtgaacc cgtacgagta agatgtccgc acgatgcgga tgttgttggc
    15061 cctgctcctg atcgccgcgt gcggcaagaa cgacgagcct ccgccggaga cgaccggagc
    15121 tcccaacgca aagcaagagt ccggtcccgg cacgcgacga ggcgccggcc cggcgaacgc
    15181 gacggagcaa gcgcacaaga tgttcgagac ggtatgcgcg atgtgccacg gcacggatgg
    15241 aaccggccgc ggccccgccg ccgagaacct caatccgaag ccgcgcaact acacggaccc
    15301 ggcgtggcaa gcgagcgtca ccgacgacga gattcgcaag acgatcctgc tcggcggtca
    15361 ggcgaccggc aagagtgcct cgatgcccgg acagccacag ctcaaagatc agcctgaagt
    15421 cctcgacggg ctcgtccaga tcatccgcgg cttcaagcag ggcgacgccc ccgccgctgg
    15481 ctctggcgca ggctcggcgg cgaaatgacg agcagcctcg cgaccagtcc cgatggccaa
    15541 tgggccgtcg cgcgcgaagg tcgcgagctc gccgtgttcg cgaatgcggc cggtgcgccg
    15601 atcgcacgac tcggcctcga tagcgacgat accgatgtcg cgctcgtcgg accgcccacc
    15661 tcactcgtgc tcgtgtcgcg cgatgagcgt cgcggttacg tcacgctgta tcagcctccc
    15721 gactttcagc tcgcggcgcg gctcgagatc gaatcgccga tgcgactcgc tgcgatcacc
    15781 gggccgcggc tcgcgctcgt ctcgccggac atgaagcagc tcgtgatcgt gcgcaccgcc
    15841 ggcaaggcgc tcgcatcgca gaagctcgag gtgacggggc cgatcgagtt tgtcgtcggg
    15901 ctcgagcgca accaggtgat gctcgggctg ctcaagaaga tcgaagtgtg ggacgccgtg
    15961 tcgggacgcc cgctgctgcg cccacagttt caattcccgc caccgccgcg cgtgatcgga
    16021 accgcgtcgg gccacatgtg ggcgatgcgc gaaggcggcg acgagatctt tgtctatcgc
    16081 ctgtcagacg gccgcccgtt tcgccactac gccggctcgc agatccgcga ggtcgtctcg
    16141 aatcccgcgt cgccggtgct gctcctcgtc accgagcgcg gcctcgtgcg cctgcattgc
    16201 tacgcacaca gcctgcagct cgtcgacggt gttccgcagc ctgcaggcgc gctcgcgctg
    16261 ctcgcgctcg gcgaagagac gttcgcgctc ggctataccg gcggcggcga tgaaccgtgg
    16321 cgcatgtcgc tcactggaac gacggcgccc gcgacgacga cgacggaagc agcggcggcc
    16381 gccaccgctg cagcgaatgc cggccaaccc gtgccgaaga gcggaacgac gccgcgcggt
    16441 cccggttggc gagacgctct cgtcgggctc gcggaccgga tcgctcaagg cggcgagctc
    16501 gacgtgccga ccatcgcgct cgacaccgag ctcggcgagc tcgcaaatcg cctcaagctc
    16561 acgccgctcg cgcgccgcgg tttgatcatc ctctatgcgc tgcacctcgc cggtgtgcgc
    16621 ggcctctcga tcgcgaagct cgccgaagcg atcggcgatt ggtctgaagc tctcgggcaa
    16681 ggcgacctcg gcgcactcgc gctcgtgaag acgaagcacg gcaacgtgcg gctgcggcgc
    16741 gccgtcaccg atgcgctcga cggcctcgca ccgcgcaacg ttcggctcgt cggcaacggc
    16801 ccgtccacgg caaagcccgg agcgttccgc gtaggccgcg agggccgcgc cgacgtcgat
    16861 gtcgaagccg agctcgtgcg ccagctcgga cgcatcgccg tcgtcgaggg ccgcctgcac
    16921 gcaggcctac tcgaagcgcg tctgcacggt gctgcggccg tcacgacctg cgttcccgac
    16981 gagaaaccgc tgccgtggcc gcgtgacgcg agcctcgtgc tcgttctcta cggcaccgcg
    17041 tcgtcgtggg tcgcggacgt ccctactctg cccagcgcta cagcttcgta acgatcgcgg
    17101 tcttcttcgc ggacttctcg aactcggact tcgcttcgtt cgcggcttcg agcgacttgt
    17161 acgagcccat gcgaacgcgg aagaactgct tgccctcgac ctcgccctcg gtgacgtagg
    17221 cctgataccc cgagcccttc accgaatcga gatacgcctg cgcctcgttc ttgtcttgga
    17281 atgacgacag ctgaagcgtg aacttctgct tcttctcttc gactttcttc tcttcgactt
    17341 tcgcttccgt cttcttctct tcgactttct tctcttcgac tttcttctct tcgaccttcg
    17401 cttcggcttt cgcctcgacc ttcttcggct cgtctttctt cggctcgtct ttcttcggct
    17461 cttccttctt cggctcggcc ttcttctcgc tcttcaccgg cgcggccggc gccttctgca
    17521 tcgactcgat cttcttctcg acgtcactcg gcgccgtctc ggtgccacgc agcgcgtctt
    17581 ggaacgacag ccgattgcca ccctcgagtc gatcgagcgc cgctagcgga tccgcggtcg
    17641 tggtgacgtt cgcgcgatcg acgtggcctc gcgcctcgac gcgtcgtccg accatgacgc
    17701 cgaggacgaa cacgaggccg acgatgacgg caccgccgaa gaacaaatag aaaatctgac
    17761 gaccatcgag gctgacctcg atcttgtctt tgtacagatc gacttcgcgc gacatgccca
    17821 cagcgtcggc aactgcgcgc cgatgggcaa gatttcgcct agctgctgat cagatggcca
    17881 ggttcagtcg acgaggttca cgacgccaat ggcgcgcgac tcgacgcgga acaccttgcc
    17941 gcgcgtcgac ggcgtggtga cggacgtcga gaggaagtcg ccctcgtcga cgacccagat
    18001 gctctgctgc ggtccgcgca gcaccttcac cgggtacgac ggcgaaatgc tgagaggcag
    18061 cggcgcgaag ccgcctgtga tgcggaacgc catctggaaa ccaggcgaga cgagcggcac
    18121 gttcgtgcgt ccaccttgcc ggtcgccatg gcactgcgcg tcgccggggt acgtcggatc
    18181 gacgaccgtc aggttgaccg cacggttgcg gaaccgaatt gcctcggcca tgcgcgtgat
    18241 gaggttctcg tctgggttgt tcagcgagca ggtattcggg atgtagttca gcatgtactc
    18301 cgtctcgtcg acggaggcct tgcacgggtt tggctcgaac gtgccgttcg gcaagcgccc
    18361 cgtcagcgga tcggccgtcg gatcacatgc gggcgcagtg agcggcaggc ggccgaccat
    18421 gaacggattc gcgttcgggt cgcggatgca gttgccgctc gtgtccgaga tgatcgagtg
    18481 caagaagccc tggcgcgagc ccacgaccga gaacgcctcg ccagcgcgca gctcgtagcg
    18541 ttgcggcgcg ttcacgcacg actgcggcgg aacgacgccc tccatgcagt accccatcga
    18601 cggcgccgct tcggcattcg cgcggcagat cgtgcccgtc gcgcagtccg tatcgctcat
    18661 gcacgccatc aggcagcgct tgccggtgcc ggccttcggc ttgcgatcgt tatcgacgcg
    18721 gcagtagtag gtgtgagcgt cctcggacgt cgtgtcgtcc ttcggattgg ccgagttgac
    18781 gttgtggatc gcgtaattcg cgaggtcgtg gcactgcgcg tcgtcgacgc atccgtcgag
    18841 cggcgtggtg cgcagctcgt gcttgcgcgg caggatctgg agctcaccgc tcttcgtgcg
    18901 gccgaccgtg tagcggcgga gcgataccaa gaagtcgcgg cacgcgttcg cgagacgctc
    18961 ggcttcgtcg gtgagcatgc atgcaccgag gcccgcgacc tggctctgcg gatggacgta
    19021 gcaggtgtac ccgaccgggc agcccgcatc accgatggct ggatcgcagc cgcgcagctg
    19081 gacgatgtcg tacggctcga cgccggcgtc gcagaacggc cgcgtctgat cgacgaggcg
    19141 catgccgccg ccgtcgacga gcgtctggct gtagcgaatg ctcgggccgt cgatcgctgc
    19201 gtcgaccttg tcgagcgaga gcgagccttc ccacgtcagc gtccagacct cgtcggcgcg
    19261 gaggccgcga agatccggga acaccgcttc acgaaccggg atgggcgccg agaacgagag
    19321 ctcggacacc ggcgcgttgc ccttggggct ctcgcacagg acctggcgaa tgcttggcag
    19381 accgccgtat ttctcgggcg cgaggacgcc tgccggaacc gtgcgcgacg ggttcgccgc
    19441 tgcgcgcgcg ccaccgctat tgcccgcgtt cgaatcggga tccggtcccg ggtcgtcgca
    19501 caccggcgtc gccggagcgc ccatcacctc ggacgcctct tccgcgatct tgccgcggtc
    19561 cggaactgca tcgcgaagct ggtgcgcgat cacgagcgga atctgcgtgc gcaggcgatc
    19621 gctcttaccg acatcggggc tgacgaagtc ggggtaatcg tcgttgtcga cgttgacgac
    19681 gtacgtctgg ccagccgccg ccgtgatcat cgcgaagtgg ccgatgagcc gcagcacgtc
    19741 gttcgtgacg ccctgcgagt cggcgcggaa gaagtcgacc gacgtcggaa cggcatcgcc
    19801 gatcagctcg atgcccgggc cgcgagcgcc cggacgacgc ggcggggtca gcgtgcatcg
    19861 cttcgtcccg gccggcgttg cgctcggatc gacgaggcag aacttgtcgc ttccgcaatc
    19921 ggtgttcgtc gtgcacgtcg cggggcttgt ctcgaacttc tggttaggga agcatgccag
    19981 gcgatcgaca tcgcggatgt ccttgatgta gcgaggatcg acctgcgtgt cgcactcgac
    20041 cggcacatcg cccgtcggat cgccgatatc gacgacgcga acggtcgagt cattggcaac
    20101 ggcgtagatg aattgatgct gcgagctctg cgccgagtca tcgatgatgc cggtcgtgcc
    20161 gcccatgccg ataaccggcg agatcgcgag tgacgtgatg ccgaggcggc cgttcgtgtt
    20221 ctggagcggc ggcaccggga tctgggacag cggacggaaa ttcgacgaat cgagatcgaa
    20281 gatcgcgacc gcgttggagt tctcggagcc gatcgcgagc acggagcggt tcgtgcgttc
    20341 gtctcgctcg aggtcgaccg tgacgggacg caaaccgggc gtgggcgcca tcgtgccgtc
    20401 gcattcggcg ggacacgtga tggcgctcgt gtcggtgacg accacgggga cgcccgtttc
    20461 gaacgagatc gcagtgacca tcgttccgct tccgacatcg acgcccgcga cgaggtgaca
    20521 gccgggatat gcgatgtatg cgatacccgt tgccgtcgac gggcacgcgt cgccgatgtt
    20581 gccgccggcg ggctcgaatg ccatcgctgc gggcttcgcg cgaatgaccg cgcctgcggc
    20641 gttcttcacc tcgaggcggg agaccgaagc ctcgcgatcc gtcggcgtat agacgcccgc
    20701 gatcgccttg ttcacgtcga ggatcgacag gtcacacgag cctgcgttcg cgatgacttc
    20761 tttgcagccg atcttgtctg tcgcgatcgc gatcggatct tcgccgacgc tgatgctgtt
    20821 cttgccgggc gtgagcgggt ccgcgtcttg aatcgcgaca tcgcctgccg agaacgtgct
    20881 cgccggcttc gcttcgaagc gcgcgacggc gaccgttccg ggtcccgact gcaggatgaa
    20941 cgcgtaccaa gacaccgacg gtaccggctg accaggacga tcttcctgac ccggtgggat
    21001 cggcttcgga gcgtcggctg cgcgttcggc ggagcggatg tcgcacgacg cgatcgcctg
    21061 cgcagatgcg acgacggagc tgtcggtgtt gagacgaagg ccgccgtaac acgcgaacgc
    21121 gacgtcgacg ggacgatcga ggttcagctg cgtcgcggca ttgttcgttg catcgctgca
    21181 cgcagcgagg acgagcgaga gtacgagcgc gcgcttcaca gcggcctcac atcgccgatg
    21241 cggagcacga cgcggttctt gcggggagag gttggcgaga catcgatcac cgcgatggtg
    21301 tcttcgagga agttcgtcac gtagaccttc ttgtgtgccg ccgagccgac cgtcgtgtac
    21361 gggccgcgtc cgacgagcac gatgtcttcg accgacgtca gaccgcgcgg gtcgacgacg
    21421 tagacctggc cgtcctggaa gcacgtcacg tacgcgcgct cgccatcgcc ggcatcgagc
    21481 acggtgagcg tcgacgcttg acggcagatg tccgtcgcgc cgaggcccat gttgcgaggg
    21541 aagcccgcgt cgttgagcga ggtgtcgtag acctgcagcg acggcgggcg acggttcagc
    21601 aggtacaggc gatcgccgcc tggggagaac gtcaggccgc gcgagtcggt cgagccgccc
    21661 gcgttcgcac cgacgaggtt caagaagaag tagttgctcg ggacgaggta cggatacacg
    21721 cgggcgccgc cgccaccgcc ttcgttcgcg ggatggccta ccgtcatcat ctgcacgcgg
    21781 tcttcgctgc ggctcgcgac gtagatgatg tcgccgcctg cgctcggccg tgccgcgacg
    21841 ccggtcgctc cgcgcagacc cgtcaacgga tccgccgcga agatcggcgt ctccttgccg
    21901 atgacatccg cgatcgccgc ggtccctccg atcggcgaat cgatcagcgt gaccgcgccg
    21961 gtcgtcaggt gcgtgacgac tgcgaactga ccattcgaat ccgcgaacgc gtcgaacggc
    22021 tcatcaggtg cagagtcgag atccggatcg tcgtgcacgt acgagagccg gtgggtctcg
    22081 tcgcattgcg agaagccttg gccgccgccg cacgtcaggt tcgtgccgtt ccaatcgatc
    22141 cacgtgatcg acggatcgcc gcgcgtcggg atgatgaggc gcgccgtgcc attgccggtg
    22201 tcctgcaccg cgatgtccgt cgcgaagttg ccgatgcgcg cgttcgcgtg ctcgacgatg
    22261 aacggctctt cgccgcacgc gagcgtctct ttgtgcgtcg ggtcgatgct gcagcccgct
    22321 tcttgcgact gcattccgac ccagccatcg gcgacgcgat cgacgtaatc gaggtcgatc
    22381 accgagaccg agccggagtc gtagcgaagc tcggagttcg cgttcgagac gaacaagaac
    22441 ttcccatcgg gcgagatggc cgcaccggtc gggaagaaca gctcgcgctc gggcgggcgg
    22501 acttcttcgg ccgaggccgt gcagcccact ccggcggcag ctccgaggag caacatgctg
    22561 aatcggcaca gggccatggg tacgggcttc gaccgcctag tacccgaaac cgttgcctgc
    22621 ttgcgttgga tcacggtttg tctcccaaga gggctgcgcg ggcggccgcg accctcgcga
    22681 tcggtactcg gaacggcgag cacgacacgt agtcgaaccc cgcgcgatgg cagaattcga
    22741 ccgagctcgg ctcgccaccg tgctcgccgc agatcccgac tttcaggccg ggtttcgtgg
    22801 ccctgccccc gcggacgccc agctcgatca gcgcccccac cccatccggg tcgaggaccg
    22861 caaacgggtc ggccggcagc gcgccgctgt cgacatacgt tggcaaaaac cgccccgcat
    22921 cgtcgcgaga caggccgaac gtcgtctgcg tcaggtcatt ggtaccgaac gaaaagaagt
    22981 ccgcatgctc ggcaatgcga tccgccacca cgcacgcgcg cggcagctcg atcatcgtgc
    23041 cgatcgtgta cggaatcgcc gcgccgccaa gcgctgcatt catcgcattc gcggtcaaca
    23101 cgcgcaggcg ccgcagctcc tcgggcacca tgacgatcgg aatcatgatc tccggaatca
    23161 cggagacgcc ctctttcgcg acgagcgctg cggcctcgcc gatcgcctgc acctggatct
    23221 cgtagatctc gggataggtg atcgcgagcc ggcagccgcg atgcccgagc atcgggttcg
    23281 cttccgtcag ctcagccacc ttcgcggcta ccttgttcgc aggctggccg agctccgcgg
    23341 caaccgcttc gatctccgcc tgcgtgtgcg gcaagaactc gtgcagcggt ggatcgagca
    23401 accgaatcgt gcacggcaac ccgtccatca cgcggaacag ctctgcaaag tcgccgcgct
    23461 gcatcggcaa gatcttcgcg agtgctgcgc gccgcccacc ctcgtcgttc gcgaggatca
    23521 tttcgcgaac agcgaggatg cgttcgggct gaaagaacat gtgctcggtg cggcacaggc
    23581 caatgccctc ggcgccgaag cttcgcgccg tcctcgcgtc ggtcggcgtg tccgcgttcg
    23641 tgcggacctt gagccggcga tattggtcga cccactgcat gagcgtgccg agctcggtgc
    23701 ctagctgcgc tggaacgagc ggcgcggagc cgaggaagac ctcgccggta gagccatcga
    23761 gcgtgagcgt gtcgccctct ttgagcttgt gctctttgcc gccaccggcg aacgtcgccg
    23821 tcttcttcgc cgcgtcgacg cgcagcgacg tgcacgccgt gatgcagcac ttgcccatcc
    23881 cgcgcgctac aacggctgcg tggctcgtca ttccgcctcg agcggtgagc gtgccggccg
    23941 cggccttcat gccgtgaatg tcttcgggtg acgtctcggt gcgaacgagc agcacttgct
    24001 cgccgcgcag atgccactct tcagcggcat tcgcggtgaa cacgacgcga ccgatcgcag
    24061 cgccgggcga tgcaggcagt cccttcgcga tcggcgtcgg gcgcgccttc ggatcgatcg
    24121 ttggatgcag aacctcgtcg agcttctgcg gatcgatgcg caggaccgct tccttcggcg
    24181 tcagcttgcc ctcggcgacg aggtcgaccg cgaccttcac catcgctttg ccggtgcgct
    24241 tgccgttgcg ggtctgcagc atgaacagct gacccttctg aatcgtgaac tcgaggtcct
    24301 gcatgtcgcg gaagtgatcc tcgaggagct tcgcgacgcg cacgagctct gcatgcgcgg
    24361 cgggcagctt cttcgcgagc tcgccgatag gctcgggcgt acggattcct gcgaccacgt
    24421 cttcgccttg cgcgttcgtc aggaactcgc cgtagagctc ggccgtgccg gtcgcgggat
    24481 tgcgcgtgaa tgcgacgccc gtcgcgcagt catcaccgag gttgccgaac accatcgcct
    24541 gcacgttgac ggcagtgccc atcgacgcag ggatgccctg catcttgcga tagacgtcgg
    24601 cgcgcgggtt gttccacgag cggaagacgg cgccgatcgc gccccacagc tgcgcgcgaa
    24661 cgtcgtcggg aaacggcgag ccggtggctt ctttgacctt cgccttgaag gccgcgacgg
    24721 cctcgcgcag cgcgtcttcc ttcagatcct gatcacggtt cgcgcggtgc ttcgacttga
    24781 tctcgtcgag gatcttgtcg aacggcggct cgtcggactc ggtcgccggg acaacgccga
    24841 ggacaacctc gccgtacatc gcgacgaaac ggcggtagca gtcccacgcg aagcgcgcgt
    24901 tgccagacag tgcggcgagc gcagcgacgg tggtgtcgtt gagcccgagg ttgaggatcg
    24961 tgtccatcat gccgggcatc gacgccggcg cgcccgagcg aacggagacg agcagcggcg
    25021 tcgtcgaatc gccgaacgtc gccttcgtga gctgctcgac cttcgacagc gcttcgttga
    25081 cttcggcgtc gaggccctcg ggatacttgt cgacgccgtg ctccatcacg tgacggcaga
    25141 cttccgtcgt gatcgtgaag ccgggcggca ccgggaggcc aagcgacgcc atctcggcga
    25201 ggtttgcgcc cttgccgccg aggagagctt tgtctttgct gcggccttcg gcagagccgc
    25261 caccgaattg acggacgtac ttcatgcggc ccccgcgagg agtcggaaat cagcgatcgc
    25321 cagatacggc gagatgaacc agttcagcaa cgcgagccgg ttgtcgcgaa gctcggcgtt
    25381 cggatccatc accatgacgc cgcccttgtc gaagaacgca gcgacggtcg gacgcagctt
    25441 ctcgagcgac tcgaacaccg cggcgtagtc gcgcttgtcg cgagcggctt tctcggcagc
    25501 ctgtgccgtc gcgatcgcgg cgtggaggtt cgtctcgacg ggcgacacga acttcgaggc
    25561 atccggcgac gtgccgatcg cgagtttttt cgcgcgcgcg tcatcgagga tgttcgcgac
    25621 gcgcttgaac acttcgcgcg cttccttgct gatcttcgcg caggcgagcg cacgggcacg
    25681 cgcgtcgact ggatcgctgg cgttctgcgc gagcgctgca tccgcatcct gcggcgggat
    25741 gccgctctcg atgaagatgc cgcgcaaacg agccttgaag aactcgtcga gcggcttgcc
    25801 gtccttgagc ttcacgcctt gcgcggcgag cgccttctcc gctgctgcgc gagcgtcgcc
    25861 aaacttatcg gtccagccgc gctctagcag gatctgccag atgccgatcg ctgcgcgacg
    25921 cagaccgaat ggatctgccg agccgctggg ctcgagaccg accgcgaagc agccgacgat
    25981 cgtgtccatg cgatcggcga tcgcgatcag cgccgcatcg tcggacttcg ggagctcggc
    26041 gccctgccct ttgggccacc agtgctgctc gatcgcagct gcgacgtcgt cgccgaagcc
    26101 ggcgatgcgt gcgtagtgct tccccatcgt gccttggacc tcggggaact cgccgacggc
    26161 gtgtgatgcg aggtcgctct tcgcgtattg cgcggccttc ttcgccgtcg ccgagccacc
    26221 gagcgcgctc acgatctcga cgaggcgttc gaccttgttg ccgatcgtct tggccttgtc
    26281 gccgagcttt gcttggaaca cgacgccgtc gagccgcttg ttccagtcgt cgaacgtctt
    26341 cttcttgtcc tcgctgaaga agaacttcgc gtcggacagg cgcgacgcga gcaccgtctc
    26401 gttaccgcgc gcgacgacgg cggggtcttt gacgatggtc gccatcatcg tggcgaatcg
    26461 cggcgcgagc ttgccgtcgg gcgtctccat cgcgaagtag cgctgatgcg tgcgcatcgc
    26521 cgtcacgatg atttcgtgcg gcacttcgag aaacgacgga tcgaactggc cgctgacgcc
    26581 gcgcgggtat tcgccgaggt tcacgacctc gtcgacgagc gcgtcgtctt tgcgaacctt
    26641 gcagcccgtc tcccgctcga gccgcgacag ctcggcgagg atctccgtct tgcgcgcttc
    26701 ttgatcgacg acgacgttcg ccgcgcgcag cgtctcgacg tacttgtcgg cgctcgcgat
    26761 ctcgatcggg ccaggcgaca ggaagcggtg gcctcgcgag gtgcgacctg acgtttggcc
    26821 ggcccacgtc agcgggatga cctcgccgcc gaacagcgcg acgagccact gcacgggacg
    26881 cacgaacgcc gtctcgctcc agccccagcg catgcctttg ggccacggga tgccggtgac
    26941 gacg
//
LOCUS       DATLBZ010000646         9658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8821355, whole genome shotgun sequence.
ACCESSION   DATLBZ010000646 DATLBZ010000000
VERSION     DATLBZ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8821355"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2678)
                     /locus_tag="VMZ53_31810"
     CDS             complement(<1..2678)
                     /locus_tag="VMZ53_31810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33146.1"
                     /translation="MSARLPGPGPQLSSGRIRVDAGRAIAKLREYQLADRTHWILEAV
                     RAAVASRAKAITLSGDANDIWLMWEGRPWELDVIPRLFDELVSPAAGGDTQHVRLLAA
                     AVNSALGMDDPSYIDVFRVAEDGTHVARYRPDVLAERTDDLEESRLAKIAVEPAKRPE
                     GAPIGMAIHLRRRVTLDLIAHFLTRPPEIAVAEAECRDIDVPLKLPDCEMHRSTSPDV
                     LRLPIGEGVDGFIAVTARATGDTQLVVAEHGVVLARYTFDLVEDIDTGRTGTLPVRVF
                     VDGSRMPTNASRSQVRRDKHPISTAEKTAKKLVPALIAKLAERVQAGDEPARCAAITW
                     IGGRIGGPQWHVDAASIRGPLRDLAKLPLVRNALGEKRGLAQHWRPEIYRGKAPLPSE
                     LTAWLSDMLWIPPGDVSVALLAGSRVDERATRRLVRWAKRQAAAQRKFFAHAKRDAKV
                     VAARNPRVRARLGAEVEGSAIEQARFTNLSGEICVYGEGTGGSLVVLLDGRELERISL
                     DSRIAFEAVIDSSRVKPADRYRGVERDAEYSRVERAVKAGVIRAIEAIAIDDVLVGYS
                     FEEPGSKAADALLARKAIGELRAQDTKIRGPLAKAPAWQTIDDRWVSVDELSAPVIGH
                     VSWDTVFTPPKGRLIIQRGDDEKLLKDVVSGRLVNYDRAHGYRETNRNVIANQLSGEL
                     VHGRPCALAVTMGDLAGAIAPAPKSTVQLRHLGVKLQTQPRKSQYVQSAVVVSSDEIV
                     PDTDWKSAHVDRGLLSKDYGGWELALVRAMARAVAGELAPELIVPGTLAIDGPMGIAL
                     CEALVAGDAHAMLGDEIIVALRKAKLVKLLGRRDRITIEELLVMYPGATQSIPLIVND
                     AQTVKSFTPMHAPQVVGEAIAILAGRKCHDATGELVR"
     gene            complement(2675..4282)
                     /locus_tag="VMZ53_31815"
     CDS             complement(2675..4282)
                     /locus_tag="VMZ53_31815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33147.1"
                     /translation="MSGGPYRDSGGDQPAEAPRDEGIVGDVIAQFADIHAFYRELVQN
                     SIDAGSPSIEVELDYDRDAQRMNVSVKDRGEGMTRDIVENQLLVLFRSTKEKDKSKIG
                     KFGIGFASVLAPNPEVVKVHTVRDKRRLTLHLYRDLSYELFDAGPATQNGTTVTLELA
                     MKPDAVKEFVHASRSALRRWCRHATVPIEFWETRADGRGGERIDGPLEIGRTLAEVRE
                     TTDAGQLTVVVGIGGMPTVGFYNHGLTLYETSHDMLLGNIAAKIQDARLGHTISRDDV
                     RRDEHFDRAVSFAREVANTKLAAEAARKLKEYAEAKRVEDVAALVEAVADAKLELPAW
                     PMPLVEPVLGATTLDVSRVHKVWASRHSTPLTEDLARLGQHVILAKDVERLASHLERP
                     TGVKIREVHGELTSIKPVERSERDEALLGTLLSILDKVYRNPGAIVLADLVGLHADRF
                     AISGLEEGAHVVSRDDAAKNPFALLGRRPLVLSIAHPLVQAARAHDDPRLAAAHLARA
                     VLLHHRLLDFGRSQEILDLALEQTGLE"
     gene            complement(4279..4809)
                     /locus_tag="VMZ53_31820"
     CDS             complement(4279..4809)
                     /locus_tag="VMZ53_31820"
                     /inference="COORDINATES: protein motif:HMM:NF040422.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HUS33148.1"
                     /translation="MLVALAGCWTSSAPVERVTPPANTAPPEARPMRFRVELERTACM
                     GRCPIYKVTIHGSGRVEWDGVENVLAIGHVDRPQLARRDLEKLARQIDDAKFFDRNEY
                     GQLETGPVCTTTGNTTTCSYSAHFCSDTTHAKITVTRNGRTHTIDNDHCDEKPGIDEL
                     EQAIDELARTGELVGR"
     gene            complement(4825..8733)
                     /locus_tag="VMZ53_31825"
     CDS             complement(4825..8733)
                     /locus_tag="VMZ53_31825"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS33149.1"
                     /translation="MAVATYPGTVSPLRGLDPFGESERDVWQGREKERDELSRLVTAD
                     TFRAGLLFGEPGVGKTSLVRAGLIPDLRDHGIVALVCEDLSQPATSFAAGLSSFGIQP
                     NAGEAPVAFIARAVSNAVAGQQFIFVVDDVDLACHDERIIGELAELYTKVVSRSAGKA
                     RFLFVCAAERLNLLSALERRTGSLFPPSNRFELPRMPQDLATQIFDRVLSLSGVAADP
                     QLAQAVVHGLDTGGGVLTADLQIAAMAMRDLKVTSIAALQKLGGATELEAAWLHDACK
                     ATGNERSALRLCAELADGPHGPRTAETVIRRTNLEASFAQSAFGVLENRGVIVRADMQ
                     GTSWMLRHEVLTPRLRVLGAPARAAARRAFDLLGSKIQNKSRLTLLELYSLRHEGITP
                     SSPQEVGVVQRSKRYYMTVAGAVAAVPIIILIIILFSMRGRVFFDLQPSPGGDHVLVK
                     GGRAGLSNFGWLPGSRYGNVYADTGLTRSMVAPEAWKKIDSQDLGASRDDWDKQIKSI
                     MAPQLAGLLDYATTGNEQTLESLKKAAKDPEDLAELLTALRPIARGGPGEIKLVEEAL
                     KMPNPAVQRAAVMVAGAAAQRGEGYQGTLVKALTSQDPELRRIAFTSVRSLGDRGKAL
                     FSAALAGDPDQAIKRELMAESSVAGAEGPSIAGAITVLSDAEASPPLVERAKAQIRGA
                     MAADALATGTALTAGLITQDHAPIPSRKFAIDLLLEQDPMPKVPNLAEAVKGAFNSKS
                     EGVKAAVLPLYAKLDPGSATTDLVAMMGDNKLQEPLRVAMALAWGQVAATNKEAAVEA
                     LDKLLGEPNNDVRAAASQAAGMLGRTYQEKLIKSVKAESYVVRKGAARGLALSAIAGA
                     SGPVAVGGIAQMWREKGTPRRDAARIFAELAKKKPGLVLDYLANASRNTEDPSLHPIG
                     VEGLCYAANVGSPEARKALQRATDDESVEVRKLVMNCVADGPDPAKNGAAIAALLIKD
                     ANGEIRGTAARVLAMTVEKGNKVSPAISTALVGLLDDPDRNVRMTGIRAVGQLGAEAP
                     KQTATDLEKLFERADQDEKLTLLRTARQIGASDLVAMAVADASPVVRTAAVDAALTAG
                     LRPGQTLSAALADADPQVRKAALERIASQKDKIDAQVLDRALSLAARDPDPELSQLAL
                     TTIARTAPKEAVQTRLKRALSSRVERVRAQAAAAAVGLVDRDAGMTASLLEPLLQDPS
                     RDVRVAMLPALAAAYSKTNNAEKLGELMRSSEGDAMRRLVAAAAFITLAKTDGGRSAS
                     EAQLKKLESGPPMARMTAKLISGLIAGKTDGMAFVQELVP"
     gene            8796..>9658
                     /locus_tag="VMZ53_31830"
     CDS             8796..>9658
                     /locus_tag="VMZ53_31830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33150.1"
                     /translation="MGGLRAFAGVLVLALAIGGALTVAHAGPGDTATKPTAQTQITVW
                     ATQNTAAQYNPYNQYNAYGGLGYGGYTPMAGAMITERRDVDVGAGGEVRITGIAATVD
                     PATVQLRSLTDPTGVAVSEQRFVPGANTPDEILARHVGESITIVTPKGEVSGTLRSVD
                     AQALVIESANSLQVLRRDGYVQDIRLPVGKSVDKPSLVWRLTAKKPGKHSVEVTYRAD
                     GLTWAADYLAIFDEGAKTIDFSAWATLRNSTGTGFDGAELTLVSGGGSAPQAYNPYMP
                     QVQRPATPVTR"
BASE COUNT         1556 a         3408 c         3080 g         1614 t
ORIGIN      
        1 cgcacgagct cgccggtggc atcgtgacac ttgcgtcccg cgaggatcgc gatcgcttca
       61 ccgacgacct gcggcgcatg catgggcgtg aagctcttca ccgtttgcgc gtcgttgacg
      121 atgagcggga tcgattgcgt cgcgcccgga tacatcacga gcagctcctc gatcgtgatg
      181 cgatcgcgcc ggccgaggag cttgacgagc ttggccttgc gcagcgcgac gatgatctcg
      241 tcaccgagca tcgcgtgcgc gtcgcccgcg acgagcgcct cgcagagcgc aattcccatc
      301 ggcccatcga tcgcgagcgt gcccggcacg atgagctccg gtgccagctc tcccgcgacc
      361 gcgcgcgcca ttgctcggac gagtgcgagc tcccagccgc cgtagtcctt ggagagcaat
      421 cccctgtcga cgtgcgcgct cttccagtct gtatcgggga cgatctcgtc ggagctgacg
      481 acgactgccg actgcacgta ctggctcttg cgcggctgcg tctgcagctt cacgccgagg
      541 tggcgcagct gcacggtcga cttcggtgcc ggcgcaatcg cacccgcgag atcgcccatc
      601 gtgacggcaa gcgcacacgg ccggccgtgc acgagctcgc cggagagctg attcgcgatc
      661 acgttgcgat tggtctcgcg atagccgtgc gcgcggtcgt aattgacgag gcgcccggag
      721 acgacatcct tcagcagctt ctcgtcgtcg ccgcgctgga tgatcaggcg gcccttcggc
      781 ggcgtgaaca cggtgtccca cgagacgtgg ccgatgaccg gcgccgaaag ctcgtcgaca
      841 ctgacccagc ggtcgtcgat cgtctgccat gccggcgcct tcgcgagcgg cccgcgaatc
      901 ttcgtgtcct gcgcgcgaag ctcgccgatc gccttgcgcg cgagcaatgc gtccgccgcc
      961 ttgctaccag gttcctcgaa cgagtagccg acgagcacgt cgtcgatcgc gatggcttcg
     1021 atcgcacgga tcacgcccgc tttgacggcg cgctcgacgc ggctgtactc cgcatcgcgc
     1081 tcgacgccgc gatagcgatc cgcgggcttc accctggacg aatcgatcac ggcttcgaat
     1141 gcgatgcggc tgtcgagcga aatgcgctcc agctcgcggc catcgaggag aacgacgagc
     1201 gagccaccgg taccttcgcc gtacacgcag atttcgccgg acagattcgt aaagcgcgct
     1261 tgctcgatcg cgctgccctc gacctctgca ccgagtcgcg cgcgcactcg cgggttacgc
     1321 gccgcgacga ccttcgcatc gcgcttggcg tgcgcgaaga acttgcgctg cgcagctgcc
     1381 tgccgcttcg cccaccgcac gagtcgccgc gttgcccgtt cgtcgacgcg cgagcccgcg
     1441 agcaaagcga ccgagacgtc gccgggcgga atccacagca tgtccgacag ccatgcggtc
     1501 agctccgacg gcaacggcgc cttgccgcga tagatctcgg ggcgccagtg ctgcgcgaga
     1561 ccgcgcttct caccgagcgc gttgcgcacg agcggcagct tcgcgagatc gcgcagaggc
     1621 ccgcgaatgc tcgccgcatc gacgtgccat tgcggaccgc cgattcgccc accgatccag
     1681 gtgatcgctg cgcagcgcgc tggctcgtcg cccgcttgca cgcgctcggc aagctttgcg
     1741 atcagcgccg gcacgagctt cttcgcggtt ttctcggcgg tcgaaatcgg atgcttgtcg
     1801 cgacggactt gcgaacgcga cgcattcgtc ggcatgcggg agccgtcgac gaacacgcga
     1861 acgggcaacg tgcctgtccg tcctgtatcg atatcctcga cgagatcgaa cgtgtagcgc
     1921 gcgagcacga cgccgtgctc cgccacgacg agctgcgtgt cgccggtcgc tcgcgcagtg
     1981 acggcgatga agccgtcgac gccttcgccg atcggaagcc gcaacacgtc gggcgacgta
     2041 ctgcgatgca tctcgcaatc gggaagcttc agcggcacat cgatgtcacg acactcggct
     2101 tcggccaccg cgatctccgg cggtcgcgtc aggaagtgcg cgatcagatc gagcgtcacg
     2161 cgacggcgga ggtgaatcgc catgccgatc ggcgcgccct cgggacgctt cgcgggctcg
     2221 acggcgatct tcgcgagccg agattcctcg aggtcgtcgg ttcgctccgc gaggacatct
     2281 ggccgatagc gcgcgacgtg ggtgccgtct tcggcgaccc gaaacacgtc gatgtacgac
     2341 gggtcgtcca tgccgagcgc gctgttcacg gccgccgcga gcaaccgcac gtgctgcgta
     2401 tcgccgccgg ccgccggact gacgagctcg tcgaacagcc gcgggatgac gtcgagctcc
     2461 cagggcctgc cctcccacat cagccagata tcgttcgcgt cgcccgagag cgtgatcgcc
     2521 ttcgcgcgcg atgcgaccgc cgcacgcacc gcctcgagga tccagtgcgt gcgatcagcg
     2581 agctgatact cgcgaagctt cgcgatcgca cggccagcgt cgacgcggat gcgtcccgac
     2641 gacagctgcg ggccaggccc tggtaaacgc gcgctcattc gagacccgtc tgctccaggg
     2701 cgaggtcgag gatttcctgc gagcgcccga aatcgagcag ccggtgatgc agcagcacgg
     2761 cgcgtgcgag atgggccgcc gcgagtcgag gatcgtcgtg cgcacgggcg gcttgcacga
     2821 gcggatgcgc gatcgacagc acgagcggcc tgcgtccgag cagtgcaaac gggttcttcg
     2881 ccgcgtcgtc gcgactgacg acatgcgcgc cctcttcgag tcccgaaatc gcgaagcgat
     2941 cggcgtggag cccgacgaga tccgcgagga cgatcgcgcc cggattgcga tagaccttgt
     3001 cgaggatcga gagcaacgtg ccgagcagcg cttcgtcgcg ctcgctgcgc tcgacaggct
     3061 tgatgctggt cagctcgccg tggacctcgc ggatcttcac gccggtcggc cgctcgagat
     3121 ggctcgcgag ccgctcgaca tccttcgcga ggatgacgtg ctggccaaga cgcgcgagat
     3181 cctcggtcag cggcgtgctg tgccggctcg cccacacttt gtgcactcgc gaaacgtcga
     3241 gcgtggtggc accgagcacg ggctcgacga gcggcatggg ccacgccggg agctcgagct
     3301 tcgcgtcggc gactgcctcg acaagcgccg cgacgtcttc gacgcgcttc gcctcggcgt
     3361 actccttcag cttgcgtgcg gcttccgcgg cgagcttggt gttggcgacc tcgcgcgcaa
     3421 acgacacggc gcgatcgaag tgctcgtcgc ggcggacgtc gtcgcggctg atcgtgtggc
     3481 cgagccgcgc gtcctgaatc ttcgcggcga tattgccgag cagcatgtcg tgactggtct
     3541 cgtagagcgt gaggccatgg ttgtagaacc ccaccgtcgg cattccgccg attccgacga
     3601 ccaccgtcag ctggcccgcg tcggttgtct ctctcacctc ggcgagcgtg cggccaatct
     3661 cgagcgggcc atcgatacgc tcgccccctc gcccgtcggc ccgggtctcc cagaattcga
     3721 tcggcaccgt cgcatgccgg caccagcgcc gcagtgcgga gcgactcgcg tgcacgaatt
     3781 ctttgacggc gtcgggcttc atcgcgagct cgagcgtcac ggttgttccg ttctgcgtcg
     3841 cgggcccggc gtcgaacagc tcgtagctga ggtcgcggta gagatgcagc gtgagccgtc
     3901 gcttgtcgcg cacggtgtga accttcacaa cctcggggtt cggcgcgaga acggacgcga
     3961 agccgatgcc gaacttgccg atcttggact tgtccttttc cttcgtcgaa cggaacagca
     4021 cgagcagctg gttctcgacg atgtcgcgcg tcatgccctc gccgcggtcc ttcaccgaga
     4081 cattcattcg ctgcgcatcg cgatcgtaat cgagctcgac ctcgatgctc ggcgacccgg
     4141 cgtcgatcga gttctgcacg agctcgcgat agaacgcgtg gatgtccgcg aactgcgcga
     4201 tgacatcgcc gacgatgcct tcgtcgcgtg gagcctcggc gggttgatcg ccgccggaat
     4261 cgcgatacgg accgccgctc atcgtccgac gagctccccg gtgcgcgcga gctcgtcgat
     4321 ggcttgctcg agctcgtcga tgcctggctt ctcgtcgcag tgatcgttgt cgatcgtgtg
     4381 cgtgcgaccg tttcgagtga cggtgatctt ggcgtgcgtc gtgtcgctgc agaagtgcgc
     4441 gctatagctg cacgtcgtcg tgttgccggt cgtcgtacag accgggccgg tctcgagctg
     4501 tccgtactcg ttgcggtcga agaacttcgc gtcgtcgatc tgacgagcga gtttctcgag
     4561 atcgcggcgc gccagctgcg gccggtcgac gtggccgatc gcgaggacgt tctcgacgcc
     4621 gtcccactcg acgcggccgc taccgtgaat cgtcaccttg tagatcggac atcgccccat
     4681 acaagcggtg cgttcgagct cgacgcgaaa gcgcatcggc cgcgcctcgg ggggcgccgt
     4741 gttcgcaggt ggcgtcaccc gctcgacggg cgcagagctc gtccagcaac cagccagtgc
     4801 gacgagcacg agcgcgcgca tcgcctaggg cacgagctct tgcacgaacg ccataccgtc
     4861 ggtcttgccg gcgatgagac cactgatgag cttcgcggtc atgcgcgcca tcggcggtcc
     4921 tgactcgagc ttcttgagct gcgcttcgct cgcggagcgg ccaccatcgg tcttcgcgag
     4981 cgtgatgaac gcagcggccg caacgagccg gcgcattgcg tcgccttccg acgagcgcat
     5041 gagctcgccg agcttctcgg cattgttcgt cttcgagtac gccgcggcga gtgcgggcaa
     5101 catcgcgacg cggacatccc gcgacggatc ttggagcagc ggctcgagta gcgacgccgt
     5161 catccccgca tcgcgatcga cgagcccgac cgcggccgcg gccgcctgcg cacgaacgcg
     5221 ctcgacgcgc gagctgagcg ctcgcttgag ccgcgtctgc acggcctctt ttggcgcggt
     5281 gcgcgcgatc gtcgtcagcg cgagctgcga aagctcggga tctggatcgc gagccgccag
     5341 tgacagcgca cgatccagca cctgcgcatc gatcttgtcc ttctgcgacg cgatgcgctc
     5401 gagcgccgcc ttgcgcacct gcggatccgc atcggcgagc gcggccgaca gcgtttggcc
     5461 cgggcgcaga cctgcggtga gcgccgcatc gacggctgcg gtgcgaacga cgggcgatgc
     5521 atcagcgacg gccatcgcca cgagatccga agcgccgatc tgccgcgccg tgcgaagcag
     5581 cgtgagcttc tcgtcctgat ccgcacgctc gaagagcttc tcgagatcgg tcgcagtctg
     5641 cttcggtgct tcggcaccga gctggccgac cgcacggatg cccgtcatgc gcacgttgcg
     5701 atcgggatcg tcgaggagac cgacaagcgc cgtgctgatc gcaggcgaga ccttgttgcc
     5761 cttctcgacg gtcatcgcga gcacgcgtgc cgccgtccca cgaatctcgc cgttggcatc
     5821 cttgatgagc agcgccgcga tcgccgcacc gttcttcgcg gggtctggcc catcggcgac
     5881 gcagttcatg acgagcttgc ggacttcgac ggattcgtcg tcggtcgcgc gctgcagcgc
     5941 cttgcgcgcc tcggggctgc cgacgttcgc tgcgtagcag agaccctcga cgccgatcgg
     6001 atgcagcgaa ggatcctcgg tattgcgcga cgcgttcgcg aggtagtcga gcacgaggcc
     6061 gggcttcttc ttcgcgagct cggcgaagat ccgcgcagcg tcacgccgcg gcgtgccctt
     6121 ctctctccac atctgcgcga tgccgccgac ggcgaccgga ccgctcgctc ctgcgatcgc
     6181 cgacagcgcg aggccacgcg cggcgccttt gcgcacgacg tagctctcgg ccttcacgct
     6241 cttgatgagc ttctcctgat acgtgcgacc gagcatgccc gccgcttgcg acgccgccgc
     6301 tcgcacgtcg ttgttcggct cgccgagcaa cttgtcgagc gcctcgacgg cagcttcttt
     6361 gttggtcgcc gcgacttggc cccacgcgag cgccatcgcg acgcgcagcg gctcctgcag
     6421 cttgttgtcg cccatcatcg ccacgagatc ggtcgtcgcg ctccccggat cgagcttcgc
     6481 gtacagcggc agcaccgccg ccttcacgcc ttccgacttc gagttgaacg cgcctttcac
     6541 cgcctcggcg aggttcggca ccttcggcat cgggtcctgc tcgagcagca gatcgatcgc
     6601 gaacttcctc gacggaattg gcgcgtgatc ttgcgtgatg agcccggccg tcagcgcggt
     6661 cccggtcgcg agcgcatccg ccgccatcgc accgcgaatc tgcgccttcg cgcgctcgac
     6721 gagtggcggc gacgcttccg catccgacag caccgtaatc gcgcccgcga tcgacggtcc
     6781 ctcggcgccg gccaccgacg actccgccat cagctcgcgc ttgatcgctt gatccggatc
     6841 gccagcgagt gccgccgaga acagcgcctt gccgcgatca ccgaggctgc gcaccgacgt
     6901 gaacgcgatg cgacgcaact ctggatcctg cgacgtgagc gccttcacga gcgtgccttg
     6961 atagccttcg ccgcgctgcg ccgctgcacc cgcgaccatc acggccgcgc gctgcaccgc
     7021 cggattcggc atcttcagtg cttcttcgac gagcttgatc tcgccgggcc cgccgcgcgc
     7081 gatcggccgc agcgccgtga gcagctccgc gagatcctcc ggatccttcg ccgccttctt
     7141 cagtgactcg agcgtctgct cgttgccggt cgtcgcgtaa tcgagcaagc ccgcgagctg
     7201 cggcgccatg atcgacttga tctgcttgtc ccagtcgtcg cgcgacgcgc cgagatcctg
     7261 cgagtcgatc ttcttccacg cctcgggcgc gaccatcgat cgcgttagcc cggtatccgc
     7321 atacacgttg ccgtagcggc tgcccggcag ccaaccaaag ttcgatagcc ccgcacgccc
     7381 acctttgacg agcacgtgat cgccaccagg actcggctgc aggtcgaaga acacgcgacc
     7441 gcgcatcgag aacaagatga tgatcaggat gatgatcggc accgccgcga ctgcgccggc
     7501 aaccgtcatg tagtagcgct tgctgcgctg aacgacgccg acttcttgcg gcgacgacgg
     7561 cgtgatcccc tcgtgccgca gcgagtacag ctcgaggagc gtcagccggc tcttgttctg
     7621 gatcttcgag ccaagcagat cgaacgcacg acgcgccgct gcccgcgccg gtgcaccgag
     7681 cacgcgcaac ctcggcgtga gcacctcgtg gcgcaacatc cacgacgtgc cctgcatatc
     7741 cgcgcgcacg atgacgccgc ggttctcgag cacgccgaac gcgctctgcg cgaacgaggc
     7801 ctccaagttg gtgcgtcgaa tgaccgtctc ggccgtgcgc ggtccgtgcg ggccgtcggc
     7861 gagctctgca cagagccgca gcgccgagcg ttcgttgccg gtggccttgc acgcatcgtg
     7921 gagccacgcc gcctcgagct ccgtcgcgcc gccgagcttt tgcaacgccg cgatcgacgt
     7981 caccttcaaa tcgcgcatcg ccatcgcagc gatctgcaga tcggccgtga gcacgccgcc
     8041 gcccgtatcc aagccatgca cgaccgcttg cgcgagctgc ggatccgcag ccacacccga
     8101 cagcgacagc acgcggtcga agatctgcgt cgccaagtcc tgcggcatgc gcggcagctc
     8161 gaagcgattg ctcggcggga acagcgaacc ggtccgccgc tcgagcgccg acagcaaatt
     8221 cagccgctcc gccgcgcaca caaacaggaa gcgcgccttg cccgccgacc ggctgactac
     8281 ctttgtatag agctcggcga gctcgccgat aatccgctcg tcgtggcacg cgagatcgac
     8341 gtcatcgacg acgaagatga actgctgccc ggcaacggcg ttgctcaccg cgcgcgcgat
     8401 aaacgcgacc ggcgcctcgc cggcattggg ctggatccca aacgaagaca acccagccgc
     8461 gaagctggtt gcaggctgac tcaaatcctc acagacgagc gccacaatcc cgtgatcgcg
     8521 caggtctgga atcagccccg cacgcacgag cgacgtcttg ccaacgcctg gctcgccgaa
     8581 cagcaagccg gcgcggaacg tgtccgctgt aaccaggcga gacagctcgt ctcgttcctt
     8641 ctcacgccct tgccagacat cgcgctcgga ttcgccaaac ggatccaagc ctcgcaaagg
     8701 cgacaccgtt cccgggtagg tcgctaccgc cacggccgca ctataaacca acatccaccg
     8761 gcactggcgc gctcggtcga ctatgctcga cgggtatggg tggacttcgt gcgtttgcag
     8821 gcgttctggt gctcgcgctg gcaatcggcg gcgcgcttac ggtcgcgcat gcaggcccag
     8881 gcgacaccgc caccaagcca acggcgcaaa cgcagatcac ggtgtgggcc acgcaaaaca
     8941 cggccgctca atacaacccg tacaaccaat acaacgcgta cggcggcctc ggttatggcg
     9001 gctacacgcc gatggccggc gcgatgatca ccgaacgccg cgatgtcgac gtcggcgccg
     9061 gcggcgaagt tcgcatcacc ggcatcgcag ccaccgtcga ccccgccacc gttcagctcc
     9121 gcagcctcac cgatccgacg ggcgtcgcgg tcagcgagca gcgcttcgtt cccggcgcga
     9181 acacgcccga cgaaatcctc gcgcgtcacg tcggcgaatc gatcacgatc gtaacgccga
     9241 aaggcgaagt cagcggcacg ctccgctcgg tcgacgcgca agcgctcgtc atcgaatccg
     9301 caaactcgct ccaagtcctg cgccgcgacg gctacgtcca agacatccgc ctccccgtgg
     9361 gcaagtccgt cgacaaaccg tcactcgtct ggcgcctcac cgccaagaag cccggcaagc
     9421 actccgtcga agtcacctac cgcgccgacg gcctcacatg ggccgccgac tacctagcga
     9481 tcttcgacga gggcgcgaag acgatcgact tctccgcatg ggcgacgctg cgcaacagca
     9541 cgggcacggg cttcgacggc gccgagctca cgctcgtcag cggcggcggg tcggcaccgc
     9601 aagcctacaa cccgtacatg ccgcaagtgc agcgccccgc cacgccggtc acgcgcta
//
LOCUS       DATLBZ010000647        13437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3623948, whole genome shotgun sequence.
ACCESSION   DATLBZ010000647 DATLBZ010000000
VERSION     DATLBZ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3623948"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VMZ53_31835"
     CDS             <1..979
                     /locus_tag="VMZ53_31835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUS33151.1"
                     /translation="QRADFETNLDIDLAYGTPDGVRYRVNLFQQRGSVGMVLRLIPPE
                     VPPFERLNLPQTVLDLIGNNHRGVILVTGATGSGKSTTLAAMIDYINTQHAFHIVTIE
                     DPIEYTFRDKRSVLNQREIGFDTMSFARALRAALRQDPDVILVGEMRDFETAQIAMTA
                     AETGHLVLSTLHTVDATETVNRIVSMFPTHQQQQARLTLASVIRGVISQRLLPRADGK
                     GMVPALEIMVNTERIREMIEEPTRTREIKDAISEGLHPYGMITFDQSLASLVKQRLVT
                     YEEAVKHSTSPSDFALLFRGVSGTSSGFAANPAGTKGADNEFELEGFEK"
     gene            996..2600
                     /locus_tag="VMZ53_31840"
     CDS             996..2600
                     /locus_tag="VMZ53_31840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33152.1"
                     /translation="MLLLLAGNAFAGRKRLVVLDLDGPKGEKFHEDLVKLLKKSHTVV
                     SVDKWNAKAEELDAAKVTEKNVKKVAKKLKIDGIVYGKVEKRRDEYIIRLKLRSGATG
                     EILGNSVQTKADGPRLDGQAQRDIKEELIGAIDELESNHDGADEEEDEAPKTTKNEED
                     EEGSKKSGFSSKKGGMDEESSKKDDEKKRKEEEKAEKKRKEEEEKAEKAEKAAKKDEE
                     ASESKKRDEEAEERPKKKKASREEEGSEGEGEGVEESVEVDDVDTALKVAPSRRAIDA
                     VVGLSFTARNLSFKYSGDLGKPPPGYKQAVPVAGALVDITAYPLSFNHKPKGIVSGIG
                     LNVLYDQVLKINSQKKYADGMGVQQTANLTTKESRWSVGGVLRYPLGTEAKSIVVGGT
                     LAYGKQVFTVAQKLPNNDPTDIPNVSYSMLSPGAFIQAPVIPKVVLNAEAMFHLITNT
                     GDIQTQQQYGPSTVTGFEFMIGADYMIMKNVFVRAGFRYETIGFKFKGTDPMAQTHTR
                     DTDADQDVQGARDTYMGGAATVGYVY"
     gene            2600..3052
                     /locus_tag="VMZ53_31845"
     CDS             2600..3052
                     /locus_tag="VMZ53_31845"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS33153.1"
                     /translation="MTIEVRPAQRADDHAVAAVSESALRTLRRVYRPTPAALARKRDL
                     AILPRLVAVVEDAVVGTVEYVAGEDRLHLMGLFVAETYRRQGVARALVDELAILARGR
                     PLSLNTIRETGNVPIFERLGFVVAAESLAVDFVGEEPLHDVLMSRASQ"
     gene            complement(3227..3946)
                     /locus_tag="VMZ53_31850"
     CDS             complement(3227..3946)
                     /locus_tag="VMZ53_31850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33154.1"
                     /translation="MQKTLPKWAFGVLSFVGVALMGSMVLNWIDIDGLFSRTGVGLAW
                     SDSHWLFLVPLAGAALLATAATRSPHTRLVAIGAGLLVAGYVLFNIAHSIVHAGLDTW
                     LILGGAGTMLAGVPSTRRAWRAVGGLMVIAGFVAPWVDYPLFKMLRHNIIDNGVGNVL
                     WAIPVGGVLGLFSVGNKGEGAKLAALGGSLVYGSLLLVIGLIAYTVFGLGAWIALGAS
                     ATALVLGVLVRDVVPGSTDDE"
     gene            4154..4729
                     /locus_tag="VMZ53_31855"
     CDS             4154..4729
                     /locus_tag="VMZ53_31855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33155.1"
                     /translation="MVSASANAPQPVPSVVAMPMPVRELPPTKAEVIQALKDRRAKNL
                     KSFIAYRKGGVYPHNSFRRGPLNVWMDAEGHLCAAATMIDKDGKHDLVEATATSNNNI
                     RLLDVVRGDLLDWILTSGFTIEEIDRIQAPQIMPDEWMLDFRQEDSKLAAGYAKTEKA
                     LVKNQKASLELAAKRLMENPHLARQLVDGML"
     gene            4751..7414
                     /gene="alaS"
                     /locus_tag="VMZ53_31860"
     CDS             4751..7414
                     /gene="alaS"
                     /locus_tag="VMZ53_31860"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HUS33156.1"
                     /translation="MKAADVRRTFLDYFKKHGHEVVASSSLVPANDPTLLFTNAGMNQ
                     FKDVFTGAETRGYKRATSSQKCVRAGGKHNDLDEVGKTPRHHTFFEMLGNFSFGDYFK
                     DDAIPFAWELLTKVYGIDAKRLVVTVYGGDKELQGVAPDDEARAIWKKVTGFGDDRII
                     GLGKKDNFWQMGETGPMGPCTEIHFNFDATGDRWPSQDPASWKGWLEIWNLVFMQFER
                     REVGGPLHKRPAPAVETGAGLERVSSVVQGVRSNYDTDLFTPILEEAAAIAGVKYGAG
                     GEADTALRVIADHARSSAFLIADGVLPGNTEREYTLRRIFRRAVRHGQQHLGIKEPFM
                     HKVCDRVIAVMSEQFPELRERQSTISKAALNEEQIFRQTLARGLTKLDTEIKNLGGAK
                     TVPGAAVFQLYDTFGFPADLTKIIAEERGLAIDEEGFRAELEQARKKSAFKAGDPAID
                     AIFKQLASEVGPTQFLGYEGRGTAGEGVIKAIVVGGKRVDSAEQGATVALVFDQTPFY
                     GNSGGQIGDTGTVLTQAAKVRVDDTEKPAGDMHVLLGEVEVGSIKVGDKVKFQVDDTR
                     RDMIRANHSATHLLHHALKKVLGDHVGQKGSLVAPERLRFDFTHFSPMTPAQVRQVED
                     IVNTEIRANHDSVIEVLPIDQARQRGAVAMFGEKYGDKVRVVSIGGESIEFCGGTHVR
                     RAGDIGMLKILSEAGIAQGVRRIEAVTGAGALEYLRKLEDELMKAGQTLKASPFEVSS
                     RVDKLLAEEKTLAREIEKLKQKIASGGGGRDLLAEAVTIKGIKVLAAAVDVDDATVLR
                     STGDQLRDKLGSGVVVLAGTGGPKVQLLAMVTKDLVGKVKAGDILGQVAAALGGRGGG
                     KPDMAQGSGGTDPSQVPAALDVARKWIEEHA"
     gene            7435..8907
                     /locus_tag="VMZ53_31865"
     CDS             7435..8907
                     /locus_tag="VMZ53_31865"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33157.1"
                     /translation="MGARLCLVLVLALAGVLGCSSKSAFHCAGSEQCVQAGMPGVCEP
                     QGFCSFADPGCAGGRRFEPNAGNGLGGTCVEVDAAMPPCGAGGQACCASEPACIAGLT
                     CNAGTCGSCIEDVAPGRHAMCVLHKDHTIWCAGENQYGQLGVGLSGPNRATWMQVRDS
                     TSLVISDAAQISTGWEFTCAVRTGGTVWCWGYAATGQMGNNTAGGAPAAVQVLKAPGQ
                     PLTNIAEVSAGQSHACARDTAGGVWCWGANNRGQIGDGTTTQRNVATPVLDAAAGPAL
                     TGALDLDVGADHACVLKAGDALWCWGINNRGQLGDGTTPPADRLSPVSVGSAVKFTTG
                     NMTTCVMRADESVLCTGDAWRNRIGNGAPVYDTISYYSTPERVLTAPGGAPLTGVDTI
                     VSGGANCALMKDTSVLCWGDDTHGQIGTGGGSTTPAPVLMADGTPLEGVDRIVTKRAR
                     VCARRTGGEWWCWGRNLEGALGDGTFVNRSVPVPLGGGCP"
     gene            8904..10367
                     /locus_tag="VMZ53_31870"
     CDS             8904..10367
                     /locus_tag="VMZ53_31870"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33158.1"
                     /translation="MIRALAIAVLVTACGSRGAFECATSAQCTRGDAIGVCIQNACAF
                     EDPACAGGYRFEANASGALAGMCTVAVDAGGSASCGGIGEACCATAPACNDSRCVSGT
                     CTACEAEIALGRRFTCVLRADHTIWCAGDNSSGQLGLGIAGVASATRIQVRASDTSLI
                     TDAIAVSAGREHACAVRTGGTVWCWGANNDGQIGNAATFPTTGNLPSIPAAVQVVKTG
                     GAPLTDIVQVEAGYEWTCARDSSGGVWCWGDNGRGQLGDNTTTRRSTAAAVLATAGTN
                     PLTGATKLLVGADMACAEGASGLVCWGANTNAQFADGTKLNHLRPFAMTATGTIASGQ
                     YHSCFLQTDSSVTCAGWSGHARLADGTIGFYDDTDVTMPRAVTRADGSPFTGVKALVA
                     GAVTCALMTDSSMQCWGDSTHGQSGNGGAKFPQPVQFRDGTPLTNVDRMLAKFAHTCA
                     HRTSGEWLCWGRNGQGDLADGSFVNRGFPIPLKGACP"
     gene            10364..11089
                     /locus_tag="VMZ53_31875"
     CDS             10364..11089
                     /locus_tag="VMZ53_31875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33159.1"
                     /translation="MSRWWLAVSLVLVTRVAHADEAGDLSKQALELYKAGKYGDAAKL
                     YERVYQLDGKPETLFSLAQSERLSGDCKKATEHYHKVIEQVSDFNVARLVQQNLKLCG
                     NDEDQQSTKPVTPPPPPLSTPPVEPRIETRTVVREHHKVDPLAITMFGVGMLALGSAG
                     GLYLAASANDSAADKAFSLEDHDDLQDRASTERLSCAVAAGVGLAFVGVAVFRWTTAK
                     DTTSNVAIAPTASGGSVVFSGRW"
     gene            complement(11090..12304)
                     /locus_tag="VMZ53_31880"
     CDS             complement(11090..12304)
                     /locus_tag="VMZ53_31880"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HUS33160.1"
                     /translation="MRSALLVALLVACSSSEMRLDRATPDYGPLAGGTRITLEGEHFP
                     VDARVFVGDREAPLAAVSRDGTKLDVVIPPGDRVGPASITVIAGSSAASSNGFRYSTP
                     PTITRVSPERVVPDTILSTLTITGTGFADEGAGDVTVLVAGVAALTVTVVDDTTLTAD
                     VALPGPVLATPSVSVIDTRGTATLDRAFRYVPSMNPGLLLFPNYFNVNASFYDFSDGS
                     VTSLGLTLQSYRFTSVFSDERGDFWAIDRGARYGLVDGAVGQIADPIALGSVITAMTP
                     YEGRILAIQRFPIALVSLNPAAGDITPYASTLSCCGSFGIAADGATVYVASRSAGVPV
                     LQTFDPETDTLGAAVTLKGAASFSVEELRVVDHVLYATSRDGTLCTIDPTTGVVTVLA
                     PIPRTNAFDVLR"
     gene            complement(12304..>13437)
                     /locus_tag="VMZ53_31885"
     CDS             complement(12304..>13437)
                     /locus_tag="VMZ53_31885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33161.1"
                     /translation="WIDGTTRCVPEAEDAIWSDAACTTAIGRGILITKPKYFIGHDLV
                     EGSAVASHVYAALDPIPAPGEYYELHDGTCTGPFSTPSEDVYYQLGGEVDTVALDESV
                     IGDGRLALRVRTSNDGSITPIGIFDRELAVDCEPVEDATGGTYCEPVYASTASLFADA
                     RCEQPAVGFVTVPSPQPLVARREQNGDCPTYHRLGAELTTPVYQRQGNACVVAGVPLP
                     HVFPLVEEAVLAPVDRAVEPSSRRLRRIIASADGLVAYSSRLVDTAIRGDCARYPVGD
                     IERCLPPTTIFVETLYEAGCTQRMRVASVPTRSCHPPAFVLSATDPPTVAALGDVTTQ
                     ATYRLESTGACSPYTPPRDRELRTLGPPLPDDTFLAARPFGER"
BASE COUNT         2516 a         4183 c         4660 g         2078 t
ORIGIN      
        1 gcaacgagcg gacttcgaaa ccaacctcga tatcgacctc gcgtacggca cgccggacgg
       61 agttcgctat cgcgtcaacc tgttccagca acgtggatcg gttggcatgg tcctgcgttt
      121 gatccctccc gaagtgccgc cgttcgagcg cctcaacctg ccgcagaccg tcctcgacct
      181 gatcggcaac aaccaccgtg gcgtgatcct cgtgacgggc gcgacgggct cgggcaagtc
      241 gacgacgctc gcggcgatga tcgactacat caacacgcag cacgcgtttc acatcgtcac
      301 gatcgaagac ccgatcgagt acacgttccg cgacaagcgc agcgtgctca accagcgcga
      361 gatcggcttc gacacgatgt cgtttgcacg cgcgctgcgt gccgcgcttc gccaggatcc
      421 cgacgtcatc ctcgtcggtg agatgcgcga cttcgagacc gcgcagatcg cgatgacggc
      481 tgccgagacc ggccacctcg tgctctcgac gctgcacacc gtcgacgcga ccgagacggt
      541 caaccgcatc gtgtcgatgt tcccgacgca ccagcagcag caagcgcgcc tcacgctcgc
      601 gagcgtaatc cgcggcgtga tctcgcagcg cctccttccg cgcgccgacg gcaagggcat
      661 ggtgcccgcg ctcgagatca tggtgaacac cgagcgcatc cgcgaaatga tcgaagagcc
      721 gacgcggacg cgcgagatca aagatgcgat ctccgaaggc ctgcatccct acggaatgat
      781 cacgttcgac cagagcctcg cttcactcgt caagcaacgc ctcgtcacgt acgaagaggc
      841 cgtcaagcat tcgaccagcc catccgactt cgcgttgttg ttccgcggcg tctccggaac
      901 ctccagtggg ttcgcggcga atccggcggg cacgaaggga gcagacaatg agttcgagct
      961 cgaaggtttt gaaaagtagt ctcgtcgtcc tcgccgtgct cctgctgctc gccggtaacg
     1021 cgttcgcagg ccgcaagcgc ttggtcgtgc tcgacctcga cggccccaag ggggagaagt
     1081 ttcacgaaga tctcgtgaag ctcctcaaga agagccacac cgtcgtctcc gtcgacaaat
     1141 ggaacgcgaa ggccgaagag ctcgatgccg cgaaggtcac cgagaagaac gtcaagaagg
     1201 tcgccaagaa gctcaagatc gacggcatcg tctacggcaa ggtcgagaag cgccgcgacg
     1261 agtacatcat tcgcctgaag ctccgctccg gggcgacggg cgagatcctc ggcaactccg
     1321 tgcagacgaa ggccgacggc ccgcggctcg acggtcaggc gcagcgcgac atcaaagaag
     1381 agctgatcgg cgcgatcgac gagctcgagt ccaaccacga tggtgcggac gaagaagaag
     1441 acgaagcgcc gaagacgacg aagaacgaag aagacgaaga aggcagcaag aagagcggct
     1501 tctcgtcgaa gaagggcggg atggacgagg agtcgtcgaa gaaggacgac gagaagaagc
     1561 gcaaagaaga agagaaggcc gagaagaagc gcaaagaaga agaagagaag gccgagaagg
     1621 ccgagaaggc cgccaagaaa gacgaagaag cgtccgagtc gaagaagcgc gacgaagagg
     1681 ccgaagagcg cccgaagaag aagaaggcct cgcgcgagga agagggcagc gaaggcgagg
     1741 gcgagggcgt cgaagagtcg gtcgaggtcg atgacgtcga tacagcgctc aaggtcgctc
     1801 cgagccgtcg cgcgatcgat gccgtcgtcg gtctgtcgtt caccgcacgc aacttgagct
     1861 tcaagtacag cggcgatctc ggcaagccgc ctccgggcta caagcaggcc gttcccgtcg
     1921 cgggcgcgct cgtcgacatc acggcgtatc cgctgtcgtt caatcacaag ccgaagggca
     1981 tcgtctcggg catcggcctg aacgtcctct acgatcaggt gctcaagatc aactcgcaga
     2041 agaagtacgc cgacggcatg ggcgtgcaac agaccgcaaa cctcaccacg aaggagagcc
     2101 gctggtcggt gggtggcgtg ctgcgctacc cgctcggaac cgaggcgaag tcgatcgtcg
     2161 tcggtggcac gctcgcgtat ggcaagcagg tgttcaccgt cgcgcagaag ctgccgaaca
     2221 atgatccgac ggacatcccg aacgtgtcgt actcgatgct ctcgccgggc gcgttcatcc
     2281 aggcgccggt gatcccgaaa gtcgtgctca acgccgaggc aatgttccac ctcatcacga
     2341 acacgggcga catccagacg cagcagcagt acggcccgtc gacggtgacc ggcttcgaat
     2401 tcatgatcgg cgccgactac atgatcatga agaacgtgtt cgtccgtgcc ggattccgct
     2461 acgagacgat cggcttcaag ttcaagggca ccgatccgat ggcgcagacg cacacgcgcg
     2521 acaccgacgc cgatcaggac gtccaaggcg cgcgcgatac gtacatgggt ggcgcagcga
     2581 ccgtcggcta cgtctactag tgacgatcga agtccggccc gcgcagcgcg cggatgacca
     2641 cgcggttgct gccgtcagtg aatccgcgct gcggacgctg cggagggtgt atcggccgac
     2701 gccggcggcg ctcgctcgca agcgcgactt ggcgatcctg ccgcggctcg tggctgtcgt
     2761 cgaagatgcg gtcgtcggca ccgtcgagta cgtcgccggc gaggaccggc tgcatctgat
     2821 gggcctcttc gtcgcggaga catatcggcg gcagggcgtt gcgcgggcgc tggtcgacga
     2881 gctcgcgatc ctggcgcgtg gccgccctct gtcgctcaac acgatccgcg agaccggcaa
     2941 tgtgccgatc ttcgagcggc tcggctttgt cgtcgcggcc gagagtcttg cggtggactt
     3001 cgtcggcgag gagcccctgc acgacgtgct gatgtcgcgc gctagtcagt aaacggcacg
     3061 ttgtcgaacg cgcagtcctc gcacagcgac ttctcgcgag gcttttcgac acgctcacca
     3121 cactgcgtgc acttcgctcg tggcggcttt gaatcttgga cggcttcctg cggagcggcg
     3181 actggcggca tgactgatta cggtgtcaac gccgcacctc gggcaactat tcgtcgtcgg
     3241 tcgagccggg aacgacgtcg cggacaagga cgccgaggac gagtgccgtc gcgctggcac
     3301 cgagcgcgat ccacgcgccg aggccgaaca ccgtgtaggc gatgaggccg atcacgagga
     3361 gcagcgagcc gtagacgagc gagccgccga gggccgcgag cttggcgcct tcgcctttgt
     3421 tgccgacgct gaacaggccg aggacgccac cgaccggaat cgcccacagc acgttgccaa
     3481 caccgttatc gatgatgttg tggcggagca tcttgaagag cgggtagtcg acccacggtg
     3541 cgacgaagcc ggcgatgacc atgaggccgc cgaccgcgcg ccatgcccgg cgagtcgagg
     3601 gtacgccggc gagcatcgtg cccgcgccgc cgaggatgag ccacgtatcg aggcccgcgt
     3661 ggacgatcga gtgcgcgatg ttgaagagga cgtagcccgc gacgaggagg cccgcgccga
     3721 tcgcgacgag gcgcgtgtgc ggcgagcgag ttgctgcggt ggcgagcagt gcggcacccg
     3781 cgagcgggac gaggaagagc cagtggctgt cgctccacgc aaggccgaca ccggtgcgcg
     3841 agaagagacc atcgatgtcg atccagttga ggaccatcga gcccatgagc gccacgccca
     3901 caaatgacag aacaccgaat gcccacttcg gaagcgtctt ttgcacggct cgacactagc
     3961 agagcggatc gcgaagtccg gcgaatagtt cgcgcattgg cgtgattgcc acctcgatcg
     4021 gcgcgcgcgc tttacacacg ccttacagac cccgtaccgg ggaggcgcgt ttgagacctt
     4081 cgcaaggaga tttcatatgt tgacccgcct cgcttcgtgt tttgtcgccc ttagtttgct
     4141 tacgactccc gcggtggttt cggcctccgc caacgcgccc cagcccgtcc cgtccgtcgt
     4201 cgcgatgccg atgccggttc gcgagctccc gcctacgaag gcggaggtaa tccaagcgct
     4261 caaagatcgc cgcgcgaaga acctcaagtc gttcatcgcg tatcgcaaag gcggcgtgta
     4321 tccgcacaac agcttccgcc gcggcccgct gaacgtgtgg atggatgccg aaggccacct
     4381 gtgtgcagcc gcgacgatga tcgacaagga cggcaagcac gacctcgtcg aggcgacggc
     4441 gacatccaac aacaacatcc gcctactcga cgtcgtgcgc ggcgacctgc tcgactggat
     4501 cctgacgagc ggcttcacga tcgaggagat cgatcgcatc caagccccgc agatcatgcc
     4561 ggacgagtgg atgctggact tccggcagga ggactccaag ctcgccgccg ggtatgcaaa
     4621 gaccgagaag gccctcgtca aaaatcagaa ggcgtctctg gagctagcgg ccaaaaggct
     4681 gatggagaac ccgcacctag cgcggcagct cgtcgacgga atgctgtaat cggggtacaa
     4741 acctcgggtc atgaaggccg cagacgttcg tcggacattc ctcgattact tcaagaagca
     4801 cggccacgag gtcgtcgcat cgagctcgct cgttccagct aacgatccga cgctcctgtt
     4861 cacgaatgcc ggcatgaacc agttcaagga cgtgttcacc ggcgcggaga cgcgtggcta
     4921 caagcgcgcg acgtcctcgc agaagtgcgt gcgtgccggt ggcaagcaca acgacctcga
     4981 tgaagtagga aagacgccgc ggcatcacac gttcttcgag atgctcggca acttctcgtt
     5041 cggcgattac ttcaaagacg atgcgattcc gttcgcgtgg gagctgctca cgaaggtcta
     5101 cggcatcgac gcgaagcggc tcgtcgtcac cgtgtacggc ggcgacaagg agctgcaggg
     5161 cgttgcgcct gacgacgaag cgcgcgcgat ctggaagaag gtcaccggct ttggcgacga
     5221 ccgcatcatc ggcctcggca agaaggacaa cttctggcag atgggcgaga cgggtccgat
     5281 gggtccgtgc accgagatcc atttcaactt cgacgcgacc ggtgaccgct ggccgtcgca
     5341 ggatccggcg tcgtggaagg gctggctcga gatctggaat ctcgtgttca tgcagttcga
     5401 gcggcgcgag gtcggcgggc ccctgcacaa gcggccggcg ccagcggtcg agacgggcgc
     5461 gggactcgag cgcgtgtcgt cggtcgtcca gggcgtgcgg agcaactacg acaccgatct
     5521 gttcacgccg atcctcgaag aggccgcggc aatcgccggt gtgaagtacg gcgcgggcgg
     5581 cgaggcggac acggcgctgc gcgtgatcgc ggatcatgcg cggtcgtcgg cgttcctgat
     5641 cgcggatgga gtgctgcccg gcaataccga gcgcgagtac acgctgcgcc gcatcttccg
     5701 gcgcgcggtc cgtcacggcc agcagcacct cgggatcaaa gagccgttca tgcacaaggt
     5761 gtgcgatcgc gtgatcgccg tcatgagcga gcagttcccc gagctgcgcg agcggcagtc
     5821 gactatcagt aaggccgccc tcaacgagga gcagatcttc cgccagacgc tcgcgcgcgg
     5881 cctcacgaag ctcgacaccg agatcaagaa tctcggcggc gcgaagaccg tgcccggcgc
     5941 ggccgtgttc cagctctacg acacgttcgg ctttcccgcg gatctgacga agatcatcgc
     6001 ggaagagcgc ggcctcgcga tcgacgaaga aggcttcaga gcggagctcg aacaggcgcg
     6061 caagaagagt gcgttcaaag cgggcgatcc ggcgatcgat gcgatcttca agcagctcgc
     6121 gagcgaggtc ggtccgacgc agttcctcgg ctacgagggg cgcggcaccg cgggcgaggg
     6181 cgtcatcaag gcgatcgtcg tcggtgggaa gcgcgtcgat tcagcagagc agggcgcgac
     6241 cgtcgcgctc gtgttcgacc agacgccgtt ctacggcaac tccggcggtc agatcggcga
     6301 caccggcacg gtgctcacgc aggcggcgaa ggtccgcgtc gacgacacgg agaagcccgc
     6361 gggcgacatg cacgtgctgc tcggtgaggt cgaagtcgga tcgatcaagg tcggcgacaa
     6421 ggtgaagttc caagtcgacg acacgcgccg cgacatgatc cgcgcgaacc actcggcgac
     6481 gcacctcctg caccacgcgc tcaagaaagt gctcggcgat cacgtcggtc agaagggctc
     6541 gctcgtcgcg cccgagcgcc tgcgcttcga cttcacgcac ttctcgccga tgacgcccgc
     6601 gcaggttcgc caggtcgagg acatcgtcaa cacggagatc cgcgcgaacc acgactcggt
     6661 gatcgaagtg ctgccgatcg atcaggcgag gcagcgcggc gcggttgcga tgttcggcga
     6721 gaagtacggc gacaaggtcc gcgtcgtttc gatcggcggc gagtcgatcg agttctgcgg
     6781 cggtacgcac gttcgccgcg ccggcgacat cggcatgttg aagatcctca gcgaagcggg
     6841 tatcgcgcag ggcgtgcgcc gcatcgaggc ggtaaccggt gcaggcgcgc tcgagtatct
     6901 gcgcaagctc gaggacgagc tgatgaaggc gggccagacg ctgaaggcgt cgccgttcga
     6961 agtgtcgtcg cgcgtcgaca agctgctcgc cgaagagaag acgctcgcgc gcgagatcga
     7021 gaagctgaag cagaagatcg cgtccggcgg cggtgggcgc gacctgctcg ccgaggctgt
     7081 gacgatcaag ggcatcaagg tgctcgcggc ggccgtcgat gtcgacgacg cgacggtgct
     7141 gcgctcgacg ggcgatcagc tgcgcgacaa gctcggctcg ggcgtcgtcg tgctcgccgg
     7201 cacgggcggt ccgaaggtgc agctccttgc gatggtgacg aaggatctcg tcggcaaagt
     7261 gaaggccggc gacatcctcg ggcaagtcgc agcagcgctc ggcggtcgtg gcggcggcaa
     7321 gccggacatg gcgcagggca gcggtgggac cgacccatcg caggtccccg ccgcgctcga
     7381 cgtcgcgcgc aagtggatcg aagagcacgc ttgagcgcgt gatacgcttg tttcgtggga
     7441 gcgcggcttt gcctcgtgct ggtgctcgcg cttgccggcg tgctcggctg ctcgtcgaag
     7501 agcgcgttcc attgcgcggg ctccgagcag tgcgtgcagg cgggcatgcc cggtgtgtgc
     7561 gagccacaag gcttctgcag cttcgccgat ccgggatgcg ccggcggccg ccgcttcgag
     7621 cccaatgccg gtaacggcct cggcgggaca tgcgtcgagg tcgatgccgc gatgccgccg
     7681 tgcggagctg gaggccaggc gtgctgcgct agtgagccgg catgtatcgc gggcctgacg
     7741 tgcaacgccg gcacgtgtgg ttcgtgcatc gaggatgtcg cgccaggccg acacgcgatg
     7801 tgcgtgctgc acaaggacca caccatctgg tgcgcgggcg agaaccagta cggccagctc
     7861 ggcgtcggct tgtccggacc gaacagagcc acgtggatgc aggttcgcga ctcgacgagc
     7921 ctcgtcatct ccgatgcggc acagatctcc acgggctggg aattcacgtg cgccgtccgt
     7981 accggcggaa ccgtttggtg ctggggctac gcggcgacgg gccagatggg caacaacacc
     8041 gctggaggcg cgcccgctgc cgttcaggtt ctcaaagcgc cgggccaacc gctgacgaac
     8101 atcgcagagg tcagcgcagg acaatcgcat gcatgtgcgc gcgatacggc cggcggtgtg
     8161 tggtgttggg gcgcgaacaa ccgcggccaa atcggcgacg gcacgacgac gcagcgaaac
     8221 gtcgcgacgc cggtgctcga tgcggctgcc gggcccgcgc tgaccggtgc cctcgatctc
     8281 gatgtcggtg cggatcacgc gtgcgtgctc aaagccggtg acgcgctgtg gtgctggggc
     8341 atcaacaatc gtggccagct cggcgacggc acgacgccac cggcggatcg cctgagcccc
     8401 gtctccgtcg gatccgcggt gaagttcacg acaggcaaca tgacgacatg cgtgatgcgc
     8461 gccgacgaga gcgtgctttg caccggtgat gcatggcgca accgcatcgg taacggcgca
     8521 ccggtctacg acaccatcag ctattactcg acgccggaac gcgtgctgac cgcgccgggt
     8581 ggtgcgccgc tcacgggtgt cgatacgatc gtcagcggcg gcgcgaactg cgcgctgatg
     8641 aaggacacga gcgtgttgtg ctggggagat gacacgcatg gtcagatcgg caccggaggc
     8701 ggctcgacca cgccggcacc cgtgctcatg gccgacggca cgccgctcga gggtgtcgat
     8761 cgcatcgtga cgaagcgagc gcgagtgtgc gcgcgtcgga ccggcggcga gtggtggtgc
     8821 tggggccgca acctcgaagg cgcgctcggc gatggcacgt tcgtcaaccg cagcgtgccc
     8881 gtaccgctcg gcggagggtg cccgtgatcc gcgcgctcgc gatcgcggtg ctcgtcacgg
     8941 catgtggctc gcgcggtgcg ttcgagtgcg cgacgagcgc gcagtgcacg cgaggcgatg
     9001 cgatcggcgt gtgcatacag aatgcgtgcg cgttcgagga tcccgcgtgc gccggtggat
     9061 atcgattcga ggcgaacgcg agcggcgcgc tcgccggcat gtgcacggtt gcggtcgatg
     9121 cgggcggctc ggcgagctgt ggtggaatcg gcgaggcgtg ctgcgcgact gcgcctgcgt
     9181 gtaatgacag caggtgcgtg tcgggaacgt gcaccgcgtg cgaagcggag atcgcactcg
     9241 gtcgccgctt cacgtgtgtg ctacgcgctg atcacaccat ctggtgcgcg ggcgacaact
     9301 cgtcgggcca gctcggcctc ggcattgccg gtgtcgcgag cgcgacgcgg atccaggtcc
     9361 gcgcatcgga tacgtcgctc atcaccgacg cgatcgcagt gtccgcgggg cgcgagcacg
     9421 cgtgtgcagt tcgcaccggc ggcaccgtat ggtgctgggg cgcgaacaac gacgggcaga
     9481 tcggcaacgc agctacgttt ccaacgacag gcaacctgcc gagcattccg gcggcggtcc
     9541 aggtcgtgaa gactggaggc gctccgctca ccgatatcgt ccaagtcgag gccggctacg
     9601 aatggacgtg cgcgcgggat agcagcggtg gcgtttggtg ttggggcgac aacgggcgcg
     9661 gccaactcgg tgacaacacg acgacgagac ggtcgactgc ggccgccgtg ctcgcgacgg
     9721 caggaacgaa tccgctgacg ggcgcgacga agctgctcgt cggtgcggac atggcgtgcg
     9781 ccgaaggtgc gagcgggctc gtgtgttggg gcgccaacac gaatgcccag ttcgccgacg
     9841 ggaccaagct gaatcatctg cgtccgttcg cgatgactgc gacgggaacg atcgcgtcgg
     9901 gccagtatca ctcgtgcttc ttgcaaactg attcgtcggt gacgtgcgcg ggatggagtg
     9961 gccacgcgcg cctcgccgac ggcacgattg gattctatga cgacacggac gtgacgatgc
    10021 cgcgcgcggt cactcgcgcg gatggctcac cgttcaccgg cgtcaaggcc ctcgtcgcgg
    10081 gcgcggtgac ctgtgcgctc atgacggatt cgagcatgca atgttggggc gacagcacgc
    10141 atggacagag tgggaacgga ggcgcgaagt ttccgcagcc ggtgcagttc cgcgacggta
    10201 cgccgctcac gaacgtcgat cgcatgctcg cgaaattcgc tcacacgtgt gcgcatcgta
    10261 cgagcggcga atggctctgc tggggacgca acggtcaggg cgacctcgcc gacggctcgt
    10321 tcgtgaaccg cggatttccg attccgctga aaggggcatg cccatgagca gatggtggct
    10381 tgcggtgtcg ctcgtgctcg tgacgcgtgt cgcgcatgca gacgaagcgg gcgatctctc
    10441 gaaacaagcg ctcgagctgt acaaagcggg caagtatggc gacgcggcga agctctatga
    10501 acgcgtctac cagctcgacg gaaagcccga gacgctgttc tcgctcgcgc aatcggagcg
    10561 cctctcggga gactgcaaga aagcgacgga gcactaccac aaggtcatcg agcaggtctc
    10621 cgacttcaac gtcgcgcggc tggtgcagca gaacttgaag ctctgcggca acgatgagga
    10681 tcagcagtcg acaaagccgg tcacgccgcc gccgccgccg ctgtccacac cgcctgtgga
    10741 accccgaatc gagacgcgca cggtcgttcg cgagcaccac aaagtcgatc cgctcgccat
    10801 cacgatgttc ggcgtcggca tgctcgcgct gggctccgcg ggtggcctgt atctcgccgc
    10861 gagcgcgaac gacagcgccg ccgacaaagc gttttcgctc gaggaccacg acgatctcca
    10921 ggaccgcgcg tcgacggaac gactcagctg cgcggtggcc gccggcgtcg gtctcgcatt
    10981 cgttggcgtc gccgtgttcc ggtggacgac cgcaaaggac acgacgagca acgtggcgat
    11041 tgccccgacg gcaagcggcg ggtcggtcgt gttcagcggc cgctggtagt cagcggagca
    11101 cgtcgaacgc gttggtgcgc gggattggcg cgagcaccgt gacgacgcct gtcgtcggat
    11161 cgatcgtgca cagcgtgccg tcgcggctcg tcgcgtagag cacgtgatcg acgacgcgca
    11221 gctcctcgac ggagaagctc gccgcgccct tcagcgtgac cgcggcgccg agcgtgtccg
    11281 tctcgggatc gaacgtctgc agcaccggca cgccggcgga gcgcgatgca acgtagacgg
    11341 tggcgccgtc ggcagcgatg ccgaacgagc cgcagcacga cagcgtcgat gcgtacggcg
    11401 tgatgtcgcc ggcggcggga ttcaacgaga cgagcgcgat tggaaagcgt tggatcgcga
    11461 ggatgcggcc ttcgtacggc gtcatcgcgg taatgacaga gccgagcgcg attggatcgg
    11521 cgatctggcc gaccgcaccg tcgacgaggc cgtagcgagc gccgcgatcg atcgcccaga
    11581 agtcgccccg ttcgtcggag aacacactcg tgaagcgata ggactgcagc gtgagcccga
    11641 gcgacgtgac gctcccatcg ctgaagtcgt agaacgacgc gttgacgttg aagtagttcg
    11701 gaaacaggag caggccggga ttcatgctcg gcacgtagcg aaatgcgcga tcgagcgtcg
    11761 ccgtgccacg cgtatcgatg acgctgacgc tcggagtcgc gagcacgggg ccgggaagcg
    11821 cgacgtcagc ggtcagcgtc gtgtcgtcga cgaccgtgac tgtcagtgcc gcaacacccg
    11881 cgacgagcac ggtaacgtcg cctgcgcctt catcggcaaa gccagtaccc gtgatcgtga
    11941 gcgtgctcag aatcgtatcg ggcacgacgc gctcgggaga cacgcgcgtg atcgtcggtg
    12001 gtgtgctgta gcgaaagccg ttgctcgatg cggcggagga gccggcaatc accgtgatcg
    12061 atgcggggcc cacgcgatcg ccgggtggaa tgacaacatc gagcttcgtg ccgtcgcgcg
    12121 agactgcggc cagcggcgct tcgcgatcgc cgacgaagac tcgcgcgtcg acggggaagt
    12181 gctcgccttc gagcgtgatt cgcgtgccgc cggcgagtgg cccgtagtcg ggcgtcgcgc
    12241 gatcgagacg catctcggag ctcgagcatg cgacgagcaa ggcgacgagg agggcagagc
    12301 gcatcaccgc tctccgaagg gccgcgcggc gaggaacgtg tcatcgggca gcggcggccc
    12361 gagcgtgcgg agctcgcgat cgcgcggcgg tgtatatggc gagcaggcgc ccgtgctctc
    12421 gagccgatac gtcgcttgcg tcgtgacgtc gccgagcgct gcgaccgttg gtgggtcggt
    12481 ggctgacagc acgaacgcag gcggatggca ggagcgcgtg gggaccgacg cgacgcgcat
    12541 gcgttgggtg caccccgctt cgtagagcgt ctcgacgaag atcgtcgtcg gtggcaagca
    12601 tcgctcgatg tcgccgactg gatagcgcgc gcaatcgccg cggatcgccg tgtcgacgag
    12661 acgcgagcta taggcgacga ggccgtcggc ggacgcgatg atgcgacgca gcctgcgaga
    12721 gctaggctcg actgcgcggt cgaccggtgc gagcaccgct tcttcgacga gcgggaacac
    12781 gtgcggcagt ggtacgcccg caacgacgca cgcgttgccc tgtcgctgat agaccggcgt
    12841 cgtgagctcg gcgccaaggc ggtggtacgt cggacagtcg ccattctgtt cgcggcgcgc
    12901 gacgagtggc tgtggcgatg ggacggtgac gaagccgaca gcgggttgtt cgcagcgcgc
    12961 gtctgcgaac agcgaagcgg tgctcgcgta caccggctcg caatacgtcc cgccggtcgc
    13021 gtcctcgacc ggctcgcagt cgacggcgag ctcgcgatcg aagatcccga tcggcgtgat
    13081 cgagccgtcg ttgctcgtgc gcacgcggag agcgaggcga ccatcgccga tcacgctctc
    13141 gtcgagcgcg acggtatcga cctctccgcc gagctggtaa tagacgtcct cggacggggt
    13201 cgagaacggt cccgtgcacg tgccgtcgtg gagctcgtag tactcgcccg gcgcgggaat
    13261 cggatcgagc gcggcgtaga cgtgcgaggc gactgccgag ccctcgacga gatcgtgacc
    13321 gatgaagtac ttcggcttcg tgatcaggat gccgcggccg atcgccgtgg tgcacgctgc
    13381 gtcgctccag atcgcatctt cagcctcggg aacgcagcgg gtggtgccgt cgatcca
//
LOCUS       DATLBZ010000648        12696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7333238, whole genome shotgun sequence.
ACCESSION   DATLBZ010000648 DATLBZ010000000
VERSION     DATLBZ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12696
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7333238"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..778
                     /locus_tag="VMZ53_31890"
     CDS             182..778
                     /locus_tag="VMZ53_31890"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HUS33162.1"
                     /translation="MSVYEPCAACRRHVLRTEAACPFCGARAKVAHRPGSSTYLGRVT
                     RSAVFAGLASCATTQPKPPPPPPVEYPFVLPPAPAEGRASISGVVREAGERRSGMLVT
                     LESDDGATKLETRTNGKGEFQFLNLAPGTYRLLPDPGYQNPRPQDGPVVQMAVAIQLV
                     ANANERTDLSVVSPQPYVRDTGPCCKPYGAPPARRRVV"
     gene            784..1968
                     /locus_tag="VMZ53_31895"
     CDS             784..1968
                     /locus_tag="VMZ53_31895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HUS33163.1"
                     /translation="MANRWVIAIAGTLMQVALGAVYAWSVFRKPLAAALGASTTEVNL
                     AFTTTIVMLGIAAFVGGLWLPKVGPRVIGMIGGALYGLGTFLAGFAEGHLVVLYVTYG
                     VVAGAGLGLGYIVPIATLVKWFPDRRGLITGVAVAGFGCGALIFGPLARHLIATHGPF
                     DAFRVIGVVYFVLVVATAAVLVNPPEGAVVARRRAHKPSVAYELSDALKTAQWYALWL
                     LLFLSVTAGISLIAEAAPMAQEVGHASEVEAAALVGTIAIFNGAGRFVWAALSDVIGR
                     RAVFVIIFVVQASVFFLLSRVHSYGAFLALACASLFCYGGGFGTMPAFAADFFGSRNV
                     GKVYGLMLTAWGAGALVGPILLSRVRDATGEYTHGLQAIAAIMIVGVIVPVFVKRPTP
                     TG"
     gene            complement(1910..2299)
                     /locus_tag="VMZ53_31900"
     CDS             complement(1910..2299)
                     /locus_tag="VMZ53_31900"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HUS33164.1"
                     /translation="MIRTIARWALTVVMVAAGANHFIATATYVGMMPAELPAHLALVY
                     ISGIAEIAGGLGLILPQTRKLAAYGLIALFIAVFPANLNMALNHLPLGTTPVPTWALW
                     ARLPLQVVFILWAWAVSRTPEQSPRRS"
     gene            complement(2347..3702)
                     /locus_tag="VMZ53_31905"
     CDS             complement(2347..3702)
                     /locus_tag="VMZ53_31905"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HUS33165.1"
                     /translation="MKRATPDELESFMKANADLEAVEAFITDPNGVARGKLLRASELA
                     ALFRSGRPLPGSILSMDITGMEVEQTGLIWDVGDADKLAFPVAGSLRRASWRSVPTAH
                     VLLGLYEPDGTPHVADPRHVLASVVGRLEAAGYAAVVAAELEFYVVDPRRGADGLLQP
                     PLTRHGRLHHIDGFSVTELGEFSPFLDELFAVARSMDLPAETAISEYGPGQLEIVLRH
                     RADALRAADEAVQWKRVVRGVAAKHDLLATFMAKPFTGRAGSGLHIHASLDDDRGHNA
                     FASPDPRGTPLMRHAIGGLLATLDESMGIFAPNASSYRRFQDASYAPISRTWGVNNRS
                     VAVRVPTGAAETRHLEHRVAGADANPYLAIAAVLAGMHKGIVERIDPGPPVEGNGYGG
                     PRLPNDWSDALRRMRASSFLETYFGARFVEIYHALKAAEYQRYNAQVTTLDLDWYLTR
                     A"
     gene            complement(3711..4466)
                     /locus_tag="VMZ53_31910"
     CDS             complement(3711..4466)
                     /locus_tag="VMZ53_31910"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUS33166.1"
                     /translation="MNIEALSRKLQTIVADHEAMRVLEATFGERLKRAPLDRDKLEWF
                     LACHVASVRSVPTYILALAMRISNDLLPHDYFNAHAVAARTLFAASHEYGLANTEAGI
                     TQTHFQLYRDGLRSWGFDERAILRDPRVFPECAEINAYIDEVAMHRPVASALACHFAL
                     EEVADREFVLLWEGFSKHWHAYGLSGPDDPALAFYQIHTVQEPLHGQYGLEALTMYLH
                     HVPTHESILVDGVVEWMDIYLRWLKAFERAFFS"
     gene            4884..5492
                     /locus_tag="VMZ53_31915"
     CDS             4884..5492
                     /locus_tag="VMZ53_31915"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HUS33167.1"
                     /translation="MNVSEEQRERVVESLSTGYAHGILEQEELERRLALVQTAKSSLE
                     LDALVTDLARAPETTALVPAKQMRVIFGSIERVGPWAVPQQLRARVLFGNLELDLRDA
                     RLSPGVTTIEVDVTMGNVEVIVPPGYQVDVEASSLLGAVEERTERVASEPTSIIRVVG
                     RVRFGHLEVSTLHRGETRRDARYRRRADRKWRRRMMRDCSGW"
     gene            complement(5493..6170)
                     /locus_tag="VMZ53_31920"
     CDS             complement(5493..6170)
                     /locus_tag="VMZ53_31920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33168.1"
                     /translation="MLEFEVLEHTESPVGVLMLRRRTAPVDGVLELALEHQLLMSSAV
                     TVSEREQATRAIAMHGGSHLDVMIGGLGLGYTAKAALDSDRVARVEVVELVQGVIDWV
                     QRGIIPLGTELFADPRFHVTQDDVFERLTRPPTTKHDILIIDVDHSPDERLGETSDSF
                     YAHANLTLAARHLKAGGVFGVWSTSPNPAFEAELHKAFREVRVDPIEFFNETTGLQET
                     NWLFLAR"
     gene            complement(6182..7837)
                     /locus_tag="VMZ53_31925"
     CDS             complement(6182..7837)
                     /locus_tag="VMZ53_31925"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HUS33169.1"
                     /translation="MEIDRRSLFRAGAATLIVGCRGSVQEAPKEPYTPPPVQPLSEPL
                     ADGVGLTNLPHPELAEVSITDLVGQLTRGELTSVALLQRYRERIAVLDDKLHSILELN
                     REAEQIAERLDRERAAGTVRGPLHGIPILLKDNIDTGDTMLTTAGSLALANTPPARRD
                     AFAVGKLRAAGAIILGKANLSEWANFRGTASCSGWSGRGGQCRNPYALDRSPSGSSSG
                     PAVATAAGLCAAGIGSETDGSIVSPANCNALVGVKPTIGLVSRSGVIPIAPSQDTLGP
                     LTRSVSDAAILLTALAGTDPEDPVTITARPEDYTKYLDRNALRGARIGVPRKGWFGVA
                     RYVDTITAAAIDTLKSLGAEIVDNLEFDVPPALGGAELTVLICEIKSAMEAYLERRGD
                     PKVRSLFDVMLYNVQNRANELRIYGHEWFEKAQVSLGMQSPGYREARALCLALARDKL
                     LDGTLQKERLDAIVCGTGGVAGLIDPVAGDMGTRGPDSTTLPAVAGYPHITVPAGWYH
                     GLPVGLSFMGGAFTEGKLLGYAYAFEQATHHRKPPRYFATAPV"
     gene            8024..8449
                     /locus_tag="VMZ53_31930"
     CDS             8024..8449
                     /locus_tag="VMZ53_31930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33170.1"
                     /translation="MRPAVIPKLSELPSEPERRNGILDSAHAEPGPEHKPTSKKAQKA
                     ETYAATAAAVLGLLLSDHKNVTIGGAAPIDENLVFEDAPKRKDGAKKKQQAGDGEGEG
                     DKDAGDKDAGDKDAGDADSKPSEAPPTRDEPLVPWVRLK"
     gene            8606..9817
                     /locus_tag="VMZ53_31935"
     CDS             8606..9817
                     /locus_tag="VMZ53_31935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HUS33171.1"
                     /translation="MLSPRICCLDLDTFFVSVERLLDPSLRGRPVIVGGERGGRGVVV
                     SASYEARASGVRSGMSVREASEKATADAVFLPGHHDLYMEYSRKTREIVERFSPIVIA
                     ASIDEHYIDFRGCERMYRAPGDTDDDATIERVVREITATIDRELGLPSSAGIATSKSM
                     AKVASGLAKPAGVLLVRAGTEAATLAPLPVRKLPGIGPVAEQKLHAEGITTLAQVVEA
                     PEAVLRRIFGSYTRGIRASARGEGIDELGRERPAFREHDPEGVVAGTISNERTFAETN
                     ADTARVILSSLCERVCSRARRRGVLAGQVTLKLRYSDFQTLTRGRTITPTNDEFELHR
                     IVLELYRETRTRRMSIRLLGVAIAKLRLDSVQLPLFEGGWRRTKAVDSIRDKFGYDAV
                     HLATTLGRRRR"
     gene            9838..10890
                     /locus_tag="VMZ53_31940"
     CDS             9838..10890
                     /locus_tag="VMZ53_31940"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33172.1"
                     /translation="MPRRPPKDDMDRDTLVDAKSTRRGVGADGAAIAGKRRRDPADAK
                     LVGTMFGAYLVQDELGGGAMGVVYRAAHIDTNRVVALKVLRQQLLDEPATVERFVREA
                     RLASRLGHPHIGGVFELVQQGERYALALELVDGEPLSSIMTMPLPPERVTLLIAQLLR
                     ALEHAHAAGLVHRDLKPDNVLVEWRNGRDHARIIDFGIAIAREGGPDSVARLTGVGQI
                     VGTPAYMSPEQARAEVLDHRSDLYSLGVMMYEMLSAELPFEGRPADVLTAKLRRDPPK
                     LEDRVPGLLVDPLLERFCFKLIARHADQRFTSARHALKTLELLATDRAAAGLELGEMD
                     VKKAMETISLPPPPKP"
     gene            complement(10950..11183)
                     /locus_tag="VMZ53_31945"
     CDS             complement(10950..11183)
                     /locus_tag="VMZ53_31945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33173.1"
                     /translation="MRPEILDAHELGRREERLVEGGVIDERVTRVGFVVVVVIDAAVH
                     LDLDVLDVVDDLDLQSKVAGIQRAQQQLGQPVL"
     gene            complement(11269..11826)
                     /locus_tag="VMZ53_31950"
     CDS             complement(11269..11826)
                     /locus_tag="VMZ53_31950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33174.1"
                     /translation="MRWRAAIVLLLVLAASPARADNDVRLHLEIDPQPFILGGYGIQP
                     GIRYNHLRAALGNFRLNVPDAVAQLGGNDGFHQHVRHSNALYILYFLDEGSGFAFGGS
                     MRLLRLSYTRDGETETARTWELGPEAIAGYKWQPTDYGFYVMPWVAIGVTLYHHGEPT
                     VAGRTYDPLPVSLFGTMNVGWDFWL"
     gene            11906..>12696
                     /locus_tag="VMZ53_31955"
     CDS             11906..>12696
                     /locus_tag="VMZ53_31955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33175.1"
                     /translation="MGRKQLGDGLRIVLVMAAAIAALGSATSGSQQTQQTQQQPAPYG
                     PMPQQPQAQQQFTPCEYACGQIARCNFSPYDACLAQCRANGTEQQPAGAQQLTTIANM
                     TCEQLQATMQGGAQPQVTQAPQQTAPTAASGQGEIETEFATPLGYTAAQQGGWAVFTP
                     SQVTEKTPCIYGLAPSRASSGSLANDAAAALLEGIPGWERSDGSSTQMKGTAPAGWSY
                     HWMMAPVRKLSNGSYQYLNATAMAFPASAGRVNILFGYGSNVHCMG"
BASE COUNT         2123 a         3989 c         4455 g         2129 t
ORIGIN      
        1 atgtcggtgc gcagcacgac gggcgcctgg accgagctcg gcacgacgcc cgtcgcgatc
       61 ccgatgacgg cactgagcgt cgagcttgcg gcggggtcgt acacgtcgac gagcgcgaac
      121 ggcgtcgcac aattcgatca cttcgtcacc tgtcactgag cgatcgagta cgatctagtg
      181 gatgagcgtc tacgagccgt gtgcggcgtg ccggcgtcac gtccttcgca ccgaggcagc
      241 gtgtccgttc tgtggagcgc gcgcgaaggt cgcgcacaga cctggcagca gcacgtatct
      301 ggggcgcgtg acgcgctcgg cggtatttgc ggggttggcg tcatgcgcga cgacgcagcc
      361 gaagccgccg ccgccgccgc ccgtcgagta tccgtttgtg ttgccgcctg cgccggccga
      421 aggtcgcgcg agcatcagcg gggtcgttcg cgaagctggc gagcgccggt cgggcatgct
      481 ggtcacgctc gagagcgacg acggtgcgac aaagctcgag acgcggacga acggaaaagg
      541 cgagttccaa tttctgaatc tcgcgccggg cacgtatcgc ctgctgccgg atccgggcta
      601 ccagaatccg cgaccgcagg acgggcctgt cgtgcagatg gccgtcgcga tccagctcgt
      661 agcgaatgcc aacgagcgca ccgacctcag cgtcgtgagc ccacaaccgt acgttcgcga
      721 taccggaccg tgctgcaagc cgtacggcgc gccgccggct cgtcgacgcg tcgtgtagtt
      781 gccgtggcga atcgttgggt catcgcgatt gcgggaacgc tcatgcaggt cgcgctcggt
      841 gcggtctatg cgtggagcgt gttccgcaag ccgctcgccg ctgcactcgg cgcgtcgacg
      901 acggaggtga acctcgcgtt cacgacgacg atcgtgatgc tcggcatcgc agcattcgtc
      961 ggtggcctgt ggctgccgaa ggtcgggcct cgcgtgatcg gcatgatcgg cggcgctctc
     1021 tacgggctcg gcacgttcct cgcgggattc gcagagggcc acctcgtcgt gctgtatgtg
     1081 acgtacggcg tcgtcgcggg tgcgggcctc ggcctcggct acatcgtgcc gatcgcgacg
     1141 ctcgtgaagt ggttccccga tcggcgcggc ctcatcaccg gcgtcgcggt cgcgggcttt
     1201 gggtgcggcg cgttgatctt cgggcccctc gcgcgacacc tcatcgcgac ccacggtccg
     1261 ttcgacgcat ttcgcgtgat cggcgtcgtc tacttcgtgc tcgtcgttgc gacggcagcg
     1321 gtgctcgtga atccgcccga gggcgcggtc gttgcgcggc gacgcgcgca caagccatcg
     1381 gtcgcgtacg agctcagcga tgcgctgaag actgcgcagt ggtacgcgct ttggctgctc
     1441 ctgttcctca gcgtcacggc gggcatctcg ctgatcgccg aggcagcgcc gatggcgcaa
     1501 gaagtcgggc acgcatcgga ggtcgaggcg gcagcgctcg tcgggacgat cgcgatcttc
     1561 aacggtgcgg ggcgcttcgt gtgggcggcg ctgtccgacg tgatcggacg ccgtgcagtc
     1621 ttcgtgatca tcttcgtcgt gcaggcgagt gtgttcttcc tgctgtcgcg cgtgcactcg
     1681 tacggcgcgt ttctcgcgct cgcatgtgcg agcttgtttt gctacggcgg cggattcgga
     1741 acgatgcctg cgtttgcggc cgacttcttc ggctcgcgca atgtcggcaa ggtctatggg
     1801 ctgatgctca cggcatgggg cgccggcgcg ctcgtcgggc ccatcctgct atcgcgcgtc
     1861 cgcgatgcga cgggcgagta cacgcacggc ctccaggcaa tcgcggcgat catgatcgtc
     1921 ggggtgattg ttccggtgtt cgtgaaacgg cccacgccca caggatgaag acgacctgca
     1981 gcggcaggcg ggcccacaat gcccacgtgg gcaccggcgt cgtgccgagc ggcaagtgat
     2041 tgagcgccat gttcaggttc gcgggaaaca ccgcgatgaa gagcgcgatc aagccatagg
     2101 ccgcgagctt gcgggtttgc ggcaggatga ggccgaggcc gcccgcgatc tcggcgatgc
     2161 ccgagatgta gacgagcgcg aggtgcgcag ggagctcggc gggcatcatg ccgacgtacg
     2221 tcgcggtggc gatgaagtgg ttggcgcccg ccgcgaccat cacgacggtc aacgcccagc
     2281 gcgcgatggt gcggatcacg ccggccggcc ctgtgcgtat tgcgagctca tcgctcgact
     2341 gtagcgtcac gcacgcgtga ggtaccagtc gaggtcgagc gtcgtgacct gcgcgttgta
     2401 gcgctggtac tcggcggcct ttagcgcgtg atagatctcg acgaagcgcg cgccgaagta
     2461 ggtctcgaga aagctcgacg cgcgcatccg gcgcagcgca tcggaccagt cattcggcag
     2521 gcgtgggcca ccgtagccgt tgccttcgac gggcggtccc gggtcgatgc gctcgacgat
     2581 gcctttgtgc atgccggcaa gcaccgcggc gatcgcgagg tacggatttg cgtcagcgcc
     2641 ggcgactcga tgttcgagat ggcgcgtctc cgcggcgccg gttggcacgc gcacggcgac
     2701 gctgcgattg ttgacgcccc acgtgcgtga gatcggcgcg tagctcgcgt cttgaaagcg
     2761 gcgatacgag ctcgcgttcg gcgcgaagat ccccatgctc tcgtcgagcg ttgcgagcag
     2821 gccaccgatc gcgtgacgca tgagcggcgt gcctcgcgga tcgggcgatg cgaatgcgtt
     2881 gtggccacgg tcgtcgtcga gactggcgtg gatgtgcagg ccactgccgg cgcggcccgt
     2941 gaaaggcttc gccatgaacg tcgcgaggag atcgtgcttg gcggcgacac cgcgaacgac
     3001 gcgcttccac tgcacggcct catcggcggc acgcagtgcg tcggcgcgat ggcgcagcac
     3061 gatctcgagc tgaccggggc cgtactcgga gatcgcggtt tcggcgggca ggtccatcga
     3121 tcgggcgacg gcgaagagct cgtcgaggaa cggcgagaat tcgccgagct ctgtgacgct
     3181 gaagccgtcg atgtggtgaa ggcgcccgtg gcgcgtcagc ggtggctgaa gcaggccgtc
     3241 agcgccgcgg cgaggatcga cgacgtagaa ttcgagctcg gccgcgacga ccgctgcgta
     3301 tcccgcggct tcgagccggc cgacgacgga tgcgaggacg tgtcgtgggt cggcgacgtg
     3361 cggagtgccg tccggctcgt agagaccgag cagcacgtgg gcagtcggga cgcttcgcca
     3421 cgacgcgcgg cgcaggcttc cagcgacggg aaacgcgagc ttgtcggcgt cgccgacatc
     3481 ccagataagg cccgtctgtt cgacctccat gcccgtgatg tccatgctga ggatggagcc
     3541 gggcaacggg cggccggagc gaaagagcgc tgcgagctcg gacgcgcgca aaagcttgcc
     3601 gcgagcgacg ccgttcggat cggtgatgaa ggcctcgacg gcttcgaggt cggcgttcgc
     3661 cttcatgaag gactccaact cgtcgggcgt cgctcgcttc atggctcacc tcacgagaag
     3721 aacgcgcgct cgaacgcctt cagccagcgc aggtagatgt ccatccactc gacgacgccg
     3781 tcgacgagga tcgactcgtg cgtgggcacg tgatgcagat acatcgtcag cgcttcgagg
     3841 ccgtactggc catggagcgg ctcctgcacg gtgtgaatct gatagaaggc gagcgccgga
     3901 tcgtcggggc ctgacaggcc gtacgcgtgc cagtgcttgc tgaagccttc ccacagcagg
     3961 acgaactcgc gatcggccac ttcttcgagg gcgaaatgac acgcgagtgc gcttgcgacc
     4021 ggtcgatgca tcgcgacttc gtcgatgtac gcgttgatct cggcgcactc cgggaacacg
     4081 cgcgggtcgc gcaggatcgc gcgttcgtcg aagccccacg agcgcaggcc gtcgcgatag
     4141 agctggaagt gcgtctgggt gatgccggct tcggtattcg cgaggccgta ctcgtgcgac
     4201 gccgcgaaca gcgtgcgcgc agcgacggcg tgcgcgttga agtagtcgtg cggcaagagg
     4261 tcgttcgaga tcctcatcgc gagcgcgagg atgtacgtcg gcaccgagcg caccgacgcc
     4321 acgtgacagg cgaggaacca ctcgagcttg tcgcggtcga gcggggcgcg cttgaggcgc
     4381 tcgccaaacg tcgcttcgag tacgcgcatc gcctcgtggt cggcgacgat ggtctgcaat
     4441 ttacgagaga gagcttcgat gttcatggac gtgtcctttc ctcagacaaa gcggtggcta
     4501 tggctctcgg gagagccctg gatatcgacg gactgcgagc tcgttagagc gagtcggtcg
     4561 gatcgccaca cgcacgaggt gtcatggaca cgctccacgt ggagacttgc aggcaagaaa
     4621 ggctaggcgc gaaacggaga cgcttcgcga cgatcggttc ggtgagctgc tgaaaatgaa
     4681 tgcgcatgtt ctgtctcctt gcgcgcggcg agaattcgcc agcgtggatc cagaatagag
     4741 cgagcgcgcg tgcgtgcaag cactttcacg caccggcgaa gacgcgctgg catctcgagt
     4801 cgaggataac ggcgacaggt ttcttcacaa tctcgatacg cttcgaaacc ggcgcgaatc
     4861 gccgcaggcg ttacatctga agcatgaacg tcagcgaaga acagcgcgag cgcgtcgtcg
     4921 agtctctctc caccggctat gcgcacggca tcctcgagca ggaggagctc gaacgccggc
     4981 ttgctctggt gcagacggcg aagagcagcc tcgagctcga tgcactcgtg acggacctcg
     5041 cgcgcgcgcc ggagacaacc gcactcgttc ccgcgaagca gatgcgcgtg atcttcggct
     5101 cgatcgagcg cgtcgggccg tgggccgtgc cgcagcaact gcgcgcgcgt gtcctgttcg
     5161 gaaatctcga gctcgacctg cgcgatgcgc gactctcgcc gggcgtaacg acgatcgagg
     5221 tcgatgtgac aatgggcaac gtcgaagtga tcgtgccgcc gggctatcag gtcgatgtcg
     5281 aagcgtcgtc gctgctcggt gctgtcgagg agcggactga acgtgtggcg agcgagccga
     5341 cgtcgatcat tcgcgtcgtc gggcgcgtgc ggttcggtca cctcgaggtg tcgacgctgc
     5401 atcgcggcga gacgcgtcgt gacgcgcggt atcgacggcg tgcggatcgc aagtggcgtc
     5461 gtcgcatgat gcgcgactgc agcgggtggt gatcagcgcg cgaggaacag ccagttcgtc
     5521 tcttgcaggc ccgtcgtctc gttgaagaac tcgatcggat cgacgcggac ctcgcgaaac
     5581 gccttgtgga gctcggcctc gaaagccggg ttcggcgagg tcgaccacac gccgaagacg
     5641 ccgccggcct tgaggtggcg agcggcgagg gtgaggttcg cgtgcgcgta gaagctatcg
     5701 ctcgtctcgc cgagtcgctc gtcgggggag tgatcgacgt cgatgatcag gatgtcgtgc
     5761 ttcgtcgtcg gaggccgcgt caggcgctcg aagacgtcgt cctgcgtgac gtgaaagcgc
     5821 ggatccgcga agagctcggt gccgagcggg atgatgccgc gctggaccca gtcgatcacg
     5881 ccttgcacga gctcgacaac ttcgacgcgc gcgacgcgat cggaatcgag cgccgctttc
     5941 gcggtgtagc cgaggccgag gccgccgatc atcacgtcga ggtgcgaacc gccgtgcatc
     6001 gcgatcgcgc gcgttgcttg ctcgcgctcg gagacggtga ccgcactgct catcaggagc
     6061 tgatgctcga gtgcgagctc gaggacgccg tcgaccggtg ccgttcgtcg cctcagcatg
     6121 agcacgccga cgggcgactc cgtgtgttct agaacttcga attcgagcac acgcagtcgt
     6181 atcacaccgg tgcggtcgcg aagtaacgcg gcggcttgcg gtggtgcgtg gcttgctcga
     6241 acgcgtatgc gtagccgagc agcttgccct ccgtgaaggc gccgcccatg aacgagagcc
     6301 cgacgggaag gccgtggtac cagccggcgg gcacggtgat gtgcggatag cctgcgacgg
     6361 cgggcagcgt cgtcgagtca ggcccgcgtg tgcccatatc accggcgact ggatcgatca
     6421 gccccgcgac gccgccggtc ccgcatacga tcgcgtcgag acgctctttt tgtagcgtgc
     6481 cgtcgagcaa cttgtcacga gcgagcgcga ggcacagcgc gcgtgcttcg cggtaccccg
     6541 gcgactgcat gccgaggctg acctgcgcct tctcgaacca ttcgtgaccg tagatgcgca
     6601 gctcgttcgc ccggttctgc acgttgtaga gcatcacgtc gaacaacgag cgcaccttcg
     6661 gatcgccgcg gcgctcgagg tacgcttcca tcgccgactt gatctcacaa atgagcacgg
     6721 tgagctccgc gccgccgagc gcgggaggaa cgtcgaattc gaggttgtcg acgatctcgg
     6781 cgccgaggct cttcagcgtg tcgatcgccg cggccgtgat cgtgtcgacg tagcgcgcga
     6841 cgccgaacca gcccttgcga ggcacgccga tgcgcgcccc gcgaagtgcg ttgcggtcga
     6901 gatacttcgt gtagtcctcg gggcgcgcgg tgatcgtgac cggatcttcc ggatccgttc
     6961 ctgcaagcgc ggtcagcaag atcgcggcat cggaaaccga gcgcgtcagt gggccgagcg
     7021 tgtcttgact cggcgcgatt ggaatcacgc ccgaacggct gacgagcccg atcgtcggtt
     7081 tgacgccgac gagcgcgttg caattcgccg gcgaaacgat tgagccatct gtctcgcttc
     7141 cgatccccgc cgcacagagg ccagcagccg tcgcgactgc cggaccgctg ctggatcccg
     7201 acggcgatcg atcgagcgca tacggattgc ggcactgccc gccgcgccca ctccaccccg
     7261 agcacgatgc ggtgccgcgg aagttcgccc actcggagag gttcgctttg ccgagaatga
     7321 tcgcgccggc agcgcgcagc ttcccgacgg caaatgcatc gcgacgtgcg ggtggtgtgt
     7381 tcgcgagcgc gagcgacccg gcagtcgtca gcatcgtatc gccggtgtcg atgttgtctt
     7441 tgagcaggat cggaatgccg tgcagtgggc cgcgcacggt tccggcggcg cgctcgcgat
     7501 cgaggcgttc ggcgatttgc tccgcctcgc gattgagctc gaggatcgag tggagcttgt
     7561 cgtcgagaac ggcgatgcgt tcgcgatagc gctggaggag cgcgaccgat gtcagctcgc
     7621 cgcgcgtgag ctgtccgacg agatcggtaa tcgagacctc ggccagctcg ggatgcggca
     7681 ggttcgtcag cccgacaccg tcggcgagcg gctcgctcag tggctgcacg ggtggcggcg
     7741 tgtacggctc cttcggcgct tcctgcaccg aaccgcggca cccgacgatc aacgttgctg
     7801 cgcccgcccg aaacaatgac cgccgatcga tctccatcac cggagcttac gcccggtcga
     7861 cctgttatga gaacgcgacg ctgtggtgga tacgccacgc gatcaccggc gcaatcagct
     7921 gatcgtgcgc tggacgtgca ggcaactccg cggcacagaa catgctgggg agccgcgcgt
     7981 gcgcgcgttg atcgttatcc tgctggcggc ttgcgccggt cccatgcggc ccgccgtgat
     8041 ccccaagctc tcggagctcc cgagcgagcc ggaacgtcgc aacggcatcc tcgacagtgc
     8101 gcacgccgag ccgggacccg agcacaagcc gacgtcgaag aaggcgcaga aagccgagac
     8161 gtacgcagcg acggcggccg ccgtgctcgg cctgctctta tcggatcaca agaacgtaac
     8221 gatcggtggc gcggcaccga tcgacgagaa cctcgtcttc gaggacgcgc cgaagcgcaa
     8281 agacggcgcg aagaagaagc agcaggcggg cgacggtgag ggcgagggcg acaaggacgc
     8341 gggcgacaag gacgcgggcg acaaggacgc tggcgacgcc gatagcaagc cgagcgaagc
     8401 tccgccaact cgcgatgagc cgctcgttcc gtgggtccgc ctgaagtagc tcgcgcgcta
     8461 ctctgccgct cgcgccggtg ctcgcgtcaa gtagggcttg ccggcggggt tgcttctagt
     8521 ccacgcagta ggcaaaacgt gcgcttttag cggcacgata cggatgttca gtgtcagagg
     8581 gctggcgtag cgtgcagcgt cgccgatgct ctcgcctcgg atttgctgcc tcgatctcga
     8641 cacgttcttc gtgtccgtcg aacggttgct cgatccatcg ctgcgcgggc ggcccgtcat
     8701 cgtcggtggc gagcgtggtg ggcgcggcgt cgtggtgtcc gcgagctacg aggcgcgggc
     8761 gtccggcgtg cgatcgggaa tgtctgttcg cgaggcgtcc gagaaagcga ctgccgacgc
     8821 cgtgtttctg ccggggcatc acgacctcta catggagtac tcgcgaaaga cccgcgagat
     8881 cgtcgagcgc ttcagcccga tcgtgatcgc ggcgagcatc gacgagcact acatcgactt
     8941 tcgcggctgc gagcgcatgt atcgcgcgcc cggcgacacg gacgacgatg caacgatcga
     9001 gcgcgtcgtc cgcgagataa cggcgacgat cgatcgcgag ctcgggttgc cgtcgagcgc
     9061 gggaattgcg acgagcaagt cgatggcgaa agtcgcgagt gggctcgcga agccagcggg
     9121 cgtgctgctc gtgcgcgccg gaaccgaggc ggccacactc gcgccgctgc ccgtgcgcaa
     9181 gctgccggga atcgggcccg tcgcggaaca gaagcttcac gccgagggaa tcacgacgct
     9241 cgcccaagtc gtcgaggcac ccgaggcagt cttgcggcgc atctttggct cgtacacgcg
     9301 cggcattcgc gcatcggcgc gcggtgaagg tatcgacgag ctcgggcgcg agcggcctgc
     9361 atttcgcgag cacgatccgg aaggcgtcgt cgccggcacg atctcgaacg agcgcacgtt
     9421 cgcggagacc aacgcagaca ccgcgcgcgt gatcctatcg agcttgtgcg agcgggtttg
     9481 ctcgcgggcg cgcaggcgtg gcgtgcttgc cggccaggtc actctcaagc tgcgctactc
     9541 ggattttcag acgctgactc gcggccgcac gatcacaccg acgaacgacg aattcgagct
     9601 gcatcgcatc gtgctcgagc tctatcgcga gacgcggacg cgtcgcatgt cgatccgctt
     9661 gctcggcgtc gcgatcgcga agctgcgact cgacagtgtc cagttgccgc tcttcgaggg
     9721 cggctggcgt cgcacgaagg ctgtggacag cattcgcgac aagttcggct acgacgccgt
     9781 ccacctcgcg acgacgctcg gccgccgcag gcgctagagt cgggtaagct cgtgggagtg
     9841 cctcgtcgac cgcccaagga cgacatggat cgcgacacgc tcgtcgacgc gaagagcacg
     9901 cgacgcggcg tcggcgcgga tggtgcggcg atcgcgggca agaggcgtcg cgatccggcg
     9961 gatgccaagc tcgtcgggac gatgttcggc gcatacttgg tgcaagacga gctcggcggc
    10021 ggcgcgatgg gcgtcgtcta tcgcgcagcg cacatcgata cgaatcgcgt cgtcgcgctc
    10081 aaggtgttgc gccagcagct cctcgacgag ccggcgacgg tcgagcgctt cgtgcgcgag
    10141 gcgcgcttgg catctcggct cgggcatccg catatcggcg gcgtgttcga gctcgtgcaa
    10201 cagggcgagc ggtatgcgtt ggcgctcgag ctcgtcgatg gcgagccgct gtcgtcgatc
    10261 atgacgatgc cgctgccgcc ggagcgcgtg acactgctga tcgcgcagct gttgcgagcg
    10321 ctcgagcatg cgcatgccgc aggcctcgtg caccgcgatc tcaaaccgga caatgtcctc
    10381 gtcgagtggc gcaacggccg cgaccacgca cgcatcatcg acttcggcat cgcgatcgcg
    10441 cgagagggtg ggccggactc ggtcgcgcgc ctgacgggcg tcgggcagat cgtcgggacg
    10501 cccgcgtaca tgtcgccgga gcaagcgcgc gcggaggtgc tggaccatcg ctcggatctc
    10561 tattcgctcg gcgtgatgat gtacgagatg ctgagcgccg agctgccatt cgagggcagg
    10621 cctgccgacg tactgaccgc gaagctccgc cgcgatccgc cgaagctgga agatcgagtg
    10681 ccgggcctgc tcgtcgatcc gctgctcgag cggttctgct tcaagctgat cgcgcgccac
    10741 gccgaccaac gcttcacgag cgcgcgccac gcactcaaga cgctcgagct tctcgcgacg
    10801 gatcgcgcgg ccgcgggcct cgagctcggc gagatggacg tcaagaaggc gatggagacg
    10861 atcagcctcc ccccgcctcc taagccgtag ccgcactttt tctgcgaccg gcgccaacgt
    10921 ggcggctcta catagagatg agggctgccc tagagaaccg gttggccgag ctgctgctga
    10981 gcgcgctgaa tccctgcgac cttggactgg aggtcgaggt cgtccacgac gtcgaggacg
    11041 tcgagatcga gatggactgc agcgtcgatg acgacgacga cgacgaaccc gacgcgggtg
    11101 acacgctcgt cgatcacccc gccttcgacg agccgctcct cgcgacgacc caattcgtgc
    11161 gcgtcgagga tctcgggacg catcagttcg tgcgcgagtc ggcgccctac gaacgcatcg
    11221 aaatccagct cgatcctgga tcgtttcgcc gcgccacgat cgaagaagct agagccagaa
    11281 gtcccagccg acgttcatcg tcccgaacaa actgacaggc agagggtcgt acgtgcggcc
    11341 cgccaccgtc ggctcgccgt ggtggtagag cgtcacgccg atcgcgaccc acggcatcac
    11401 gtagaagccg tagtcggtcg gttgccactt gtagccggcg atcgcttcgg ggccgagctc
    11461 ccacgttctc gcggtttcgg tctcgccgtc gcgcgtgtac gagaggcgca gtagccgcat
    11521 cgagccaccg aacgcgaagc cgctgccttc gtcgagaaag tagaggatgt acagcgcgtt
    11581 cgagtgacgc acgtgctggt gaaagccgtc gttgccgccg agctgcgcga ctgcatccgg
    11641 gacgttcagg cggaagttgc cgagtgcggc gcgcaggtgg ttgtagcgaa tgccaggttg
    11701 gatgccgtag ccgccgagga tgaacggctg cggatcgatc tcgaggtgga ggcgaacgtc
    11761 gttgtccgcc cgcgcggggc tcgcggcgag cacgagaaga aggacaattg cggctctcca
    11821 gcgcatgttg gagtcgtacc gcagggcgcg acgatttccg cgcgacaaat cccgcgatgt
    11881 tcctccttct tctatggtcc ggaggatggg acgtaagcag ttgggggatg ggctgcgcat
    11941 cgtgctcgtg atggccgcgg cgattgccgc gttgggatcg gcgacgtctg gctcgcagca
    12001 gacgcagcag acgcagcagc aacccgcgcc gtatgggccg atgccgcagc agccgcaagc
    12061 gcagcagcag ttcacgccgt gcgagtacgc gtgcgggcag atcgcgcgct gcaacttcag
    12121 cccctacgat gcgtgcctcg cgcagtgccg cgcgaacggg accgagcagc agcccgccgg
    12181 cgcgcagcag ctcacgacga tcgcgaacat gacctgcgag cagctgcagg ccaccatgca
    12241 aggtggtgct cagccgcagg taacgcaggc gccgcagcag accgctccga cggcggcgag
    12301 cgggcagggc gagatcgaga cggagttcgc gacgccgctt ggctacacgg cggcacagca
    12361 aggcggctgg gctgtgttca cgccgtcgca ggtgaccgag aagacgccgt gcatttatgg
    12421 tctcgcgccc tcgcgcgcgt cgtccggatc gctcgcgaat gatgcagcgg cggctttgct
    12481 cgaggggatt cccggctggg aacgcagcga cggctcgtcg acgcagatga aaggcaccgc
    12541 gcccgcaggg tggtcgtatc actggatgat ggcgccggtg cgcaagctca gtaacggcag
    12601 ctatcagtat ctcaacgcga cggcgatggc gtttccggcg agcgcgggtc gcgtcaacat
    12661 tctgttcggc tacggcagca acgtgcactg catggg
//
LOCUS       DATLBZ010000649         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6460573, whole genome shotgun sequence.
ACCESSION   DATLBZ010000649 DATLBZ010000000
VERSION     DATLBZ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6460573"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VMZ53_31960"
     CDS             <1..384
                     /locus_tag="VMZ53_31960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33176.1"
                     /translation="SCYVERTCADGRVVASDFLECAAEQSHRCFTKSSRIYKDDIAYL
                     SEPELDALAAQIADLKLARYRYKDEPGNRLGFIIEDAPDAPWVSTDHRRVDLYALLAS
                     SIAALQQQDARIRQLEQDIASCKQR"
     gene            415..1542
                     /locus_tag="VMZ53_31965"
     CDS             415..1542
                     /locus_tag="VMZ53_31965"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUS33177.1"
                     /translation="MTKQLFAPLADPDHVARLRAFVAEHIAPTAAAIDRDDRFPVDEV
                     KALARAGFMSTAVPRQYGGSDRSFVDTVAVFEEVAVASAAVGASLITTFLAQTCLRLS
                     GTEAAKARYLPRFNDGLVASFAMTEAAHGSDVKRLDTKARRTPDGWILDGAKAYVTSS
                     SAAELFVILADAEGGITAFVVPRDAPGVSVEPATDDVHAFGLRNGPHCTVRLTAARIP
                     LDCMLGEEGNGIRHAATSLDYSRTLAAAITLGIARAATTGALEWARTRKAGGKPIIEH
                     QGIAWYFAELVTEIDAARLLTYAAARAVEQERDIERLPAQAKLMASRVAVHAATQAIQ
                     ICGAQGMLETQPFGRYLRDAKAYEIAGGSSEILKNTIAKHL"
     gene            1571..>2412
                     /locus_tag="VMZ53_31970"
     CDS             1571..>2412
                     /locus_tag="VMZ53_31970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUS33178.1"
                     /translation="MSVSETTAKWVEELFDKSIVPTLVEYIKIPNKSAMFDPDWKKNG
                     HVDRAVDLLANWAKANLPDGAKLEVVQLEDKGKPRTPCIFIEIPGTGGKQGDTVMLYG
                     HLDKQPEMTGWREGLGPWTPVIEGDKLYGRGGADDGYAIFASLTAINAVRRDKFPHSR
                     CVVLIEACEESGSYDLPAYIEHLAPRIGDLSLVVCLDSGCANYDQLWSTTSLRGLVIG
                     TLEVSLLTEGVHSGDGTGVIAASERVIRQLLDRIDDSHTGVVKIPSLATQIPKQRVLQ
                     AERTA"
BASE COUNT          436 a          821 c          779 g          376 t
ORIGIN      
        1 agctgttacg tcgagcgcac gtgcgctgat ggccgcgtcg tcgcgagcga cttcctcgag
       61 tgcgccgcgg agcagagcca ccgctgcttc acgaagtcgt cgcgcatcta caaggacgac
      121 atcgcgtacc tcagcgagcc cgagctcgac gccctcgccg ctcagatcgc cgacttgaag
      181 ctcgcgcggt atcgctacaa agacgagccc ggcaatcgcc tcggcttcat catcgaggat
      241 gctcccgacg cgccgtgggt ctcgaccgat catcgccgcg tcgatctcta cgccctgctc
      301 gcgtcgtcga tcgctgcgtt gcaacaacaa gacgcacgca tccgccagct cgaacaggac
      361 atcgcctcgt gcaagcaacg atgagcggcc atcgcgacat cccatgagcg agcgatgacg
      421 aaacagctgt tcgcccccct cgcggatcca gaccacgtcg cgcggttgcg cgcattcgtc
      481 gccgagcaca tcgcaccgac agcagcggcg atcgaccgcg atgatcgatt tcccgtcgac
      541 gaagtgaagg cgctcgcacg tgcaggcttc atgtccaccg ccgtgccacg gcagtacggc
      601 ggcagcgatc gatcattcgt cgacaccgtc gcggtcttcg aggaagtcgc cgttgcaagc
      661 gccgccgtgg gcgcgagcct catcacgacg ttcctcgcgc agacctgctt gcgactgtcc
      721 ggcaccgagg cggcgaaggc gcgctatctc ccgcgcttca acgacggcct ggtcgcgagc
      781 ttcgcgatga ccgaggcggc gcacggctcc gatgtgaagc ggctcgatac caaggcgcgg
      841 cgcacgcccg acggttggat cctcgacggc gcgaaggcgt acgtcacctc gagctccgct
      901 gcggagctgt tcgtgattct cgcggacgcg gaaggcggca tcaccgcatt tgtcgtgccg
      961 cgggacgcgc ccggcgtgag cgtcgagcct gcgaccgacg acgttcacgc gttcggcctg
     1021 cgcaacggcc cgcactgcac cgtgcgcctg accgctgcgc gcattccact cgattgcatg
     1081 ctcggcgagg aaggcaacgg catccgccac gccgcgacga gcctcgatta ttcgcgcacg
     1141 ctcgcggcgg cgatcaccct cggcatcgcg cgcgcggcaa cgacgggagc gctggagtgg
     1201 gcgcgcacgc gcaaggccgg cggcaagccg atcatcgagc atcaaggcat cgcgtggtac
     1261 ttcgcggagc tcgtcacgga gatcgatgcc gcgcggttgc tgacgtatgc ggcggcgcgc
     1321 gccgtcgagc aagagcgcga catcgagcga ctcccggcgc aggccaagct catggcgagc
     1381 cgcgtcgcgg tgcatgcggc cacgcaggcg attcagattt gcggcgcgca aggcatgctc
     1441 gagacgcagc cgtttggccg gtatctgcgc gatgcgaagg catacgaaat cgccggtggc
     1501 tcgtcggaaa tcctgaagaa cacgatcgcc aagcacctct agcggccgcc cccgcagtat
     1561 ggtgccgcgc atgtcggtca gcgaaaccac cgcgaagtgg gtcgaggaac tattcgacaa
     1621 gtcgatcgtt ccgacgctcg tcgagtacat caagattcca aacaagtcgg cgatgttcga
     1681 tcccgactgg aagaagaacg gtcacgtcga tcgcgcggtc gacttgctcg cgaactgggc
     1741 gaaggcgaac ttgcccgacg gcgcgaagct cgaggtcgtt cagctcgagg acaagggcaa
     1801 gccgcgcacg ccgtgcatct tcatcgagat tccaggcacg ggcggcaagc agggcgacac
     1861 cgtcatgctg tacggccatc tcgacaagca gccggagatg acgggctggc gcgaaggact
     1921 cggcccgtgg acgccggtca tcgagggcga caagctctac ggtcgcggcg gcgccgacga
     1981 cggctacgcg atctttgcgt cgctcacggc gatcaacgcc gttcgtcgag acaagttccc
     2041 gcactcgcgc tgcgtcgtct tgatcgaagc atgcgaggag agcggcagct acgatctgcc
     2101 cgcgtacatc gagcatctcg cgccacgcat cggcgacctc tcgctcgtcg tttgcctcga
     2161 ctctggctgc gcgaactacg accagctctg gtcgacgacg tcgctgcgcg gcctcgtgat
     2221 cggcacgctc gaagtgtcgc tgctcaccga gggcgtccac tcgggcgacg gcaccggtgt
     2281 gatcgcggcc agcgagcgag tgattcgcca gctgctcgat cgcatcgacg atagccacac
     2341 cggcgtcgtc aaaatcccgt cgctcgcgac gcagattccg aagcagcgcg tgctgcaggc
     2401 cgagcgcacc gc
//
LOCUS       DATLBZ010000650         3886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3635970, whole genome shotgun sequence.
ACCESSION   DATLBZ010000650 DATLBZ010000000
VERSION     DATLBZ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3635970"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VMZ53_31975"
     CDS             complement(<1..952)
                     /locus_tag="VMZ53_31975"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HUS33179.1"
                     /translation="MSSNIVVYGATGLVGGRVCTALDAAEVPFIAAGRRRMALDKLAR
                     LVEPAGVRVAEIEHAALVKAFEGASVVINCAGPLAEMGEAVLVAALEAGAHYVDIGGD
                     QAFLHDIYERYESSARKAGKVVVPGCGLNCAIGDWAAAWASAHVCGVADEGDVVRAAQ
                     APRLGEDQPLDEVAVSYLFDDLVLSPASQKAVFGNLHTRGLAWRRDRWETVTPGIEKK
                     RVNAGPEMGGERDAVSFPGGDVVTVPRHIAANRVQTYLSMTRSQVATTALRFLARAMP
                     FVPKRATDLLAAYQPAEDEYARTHFAVVAQARRRFDAAQIV"
     gene            complement(942..2366)
                     /locus_tag="VMZ53_31980"
     CDS             complement(942..2366)
                     /locus_tag="VMZ53_31980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33180.1"
                     /translation="MSRAACIALCLLAARAHASPRSDPTIGRTVFTGATVQHPTSIEL
                     NPAALGVGAKNEIYIAALAAVDQFKIDHRGVTTTGHVSDTTLSPGGTLAGLWHTGTDG
                     KITLAGQFRSAPAERFLESHDPLQYSILGGYHRTGSADIAASFRITNRWYFGIGLSAQ
                     ISWLRLRYARDTALEAGRDPARGIDSDCEGSPCGIENASATEVYDVDVNTGILSASNV
                     IGATIGTLVKLPRDIWLGASYHLPPGLAVQNTLTGTIAVERAPRDGGNTITGASTVYL
                     SQPASVDVEVRKLLVSDIDLHIGGRWEDLSRMSTYDVRAFGSTFAAQGIPEWMPRARG
                     FHDSYALWGGIEQVETGQFVRGGGRIGFETSTIPDERTTAWTIQPASLTLDGGVQVLL
                     VDRGVGHARWLLQLTYGLQYFPTVSVDNSEFDPNSRLTCYDSGFDYSTAACNETRKGY
                     GLPTANGDYSRIEHAFRLGLRYEL"
     gene            2401..3708
                     /locus_tag="VMZ53_31985"
     CDS             2401..3708
                     /locus_tag="VMZ53_31985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33181.1"
                     /translation="MLARLVPIAFAAMLLTAGCEKTDHATIDKWMGTKKGPSKLKKTL
                     VDDSLDPDLSAHAAANMIKKGRDQEVRTELDNMSVPRKQAIVGKLAPRLWEMARIEGE
                     MMMPAPQQVQAKDALVSIRKYADTPMRTTIDGYLIDWYTVPSYEGRSKAGAVLGEAVM
                     RMVGPGAAKKLMRVADGIIAAPGQDKTKKRIGDQLLLGLAATGDPDAVKYILDIAKMD
                     RGDETLAKRALGALYTAYVDPQGLFDLQEPGALVPNLDQLVSIAKSDSMDPAAANSAV
                     ALIRVVGPPQCLQPLVSMIAHPHPDPRFRYFGPDAALKCGGVKAIKQVIQAMPDQAYE
                     KVKLEGTVVLDITKMSPRDQVLAELRDLLNDKSRMARWVAIETLAAMKSKEDAPAIAK
                     VKGGEKLLGFWGDQSGTDPAARKSDPTLAQRAKELSDALQKAP"
BASE COUNT          713 a         1249 c         1299 g          625 t
ORIGIN      
        1 cgacgatctg cgcagcatcg aatctgcgcc gcgcttgcgc gacgacggcg aagtgcgtgc
       61 gcgcgtattc gtcctcggca ggctgatagg cggcgaggag atccgtcgcg cgcttcggta
      121 cgaacggcat cgcgcgcgcg agaaagcgca gcgctgtggt ggccacttgg ctacgcgtca
      181 tcgacaaata tgtctgcacg cggttcgctg cgatgtgacg tggcaccgtg acgacgtcgc
      241 cgccgggaaa cgacacggcg tcgcgttcgc cgcccatctc ggggcccgca ttgacgcgct
      301 tcttctcgat gcccggcgtg accgtctccc agcggtcgcg acgccacgcg aggccgcgcg
      361 tatggagatt gccgaacacc gccttctggc tcgcgggcga gagcacgaga tcgtcgaaca
      421 ggtagctcac cgcgacttcg tcgagcggct ggtcctcgcc gagcctcggt gcttgcgcag
      481 cgcgcacgac atcaccttcg tcggcaacgc cgcagacatg cgcggacgcc caggcagcag
      541 cccaatcgcc gatcgcgcag ttgagcccgc aaccgggcac gacgaccttg ccggctttgc
      601 gcgcgctcga ttcgtagcgc tcgtagatgt catgcaggaa tgcttggtcg ccgccgatgt
      661 cgacgtagtg cgcgcccgct tcgagcgcag cgacgagcac cgcttcgccc atctccgcga
      721 gagggcccgc gcagttgatc acgaccgatg cgccctcgaa tgctttcacc agcgctgcgt
      781 gctcgatctc ggcgacgcgc acgccagccg gctcgacgag acgcgcgagc ttatcgagtg
      841 ccatccgccg ccggcccgct gcgatgaacg gaacctccgc ggcatcgagc gcggtgcaca
      901 cgcgaccgcc gacgagcccc gtcgcgccgt agacgacgat gttagagctc atagcgaagt
      961 ccgagacgaa atgcgtgctc gatacgcgag tagtcgccgt tcgcggtcgg aagtccgtag
     1021 cccttgcgtg tctcgttgca tgcggccgtc gagtagtcga agccggagtc gtagcaggtg
     1081 agtcgcgaat tgggatcgaa ctcgctgttg tcgacgctga ccgtcggaaa atattgcagc
     1141 ccatacgtga gctgaagcag ccatcgcgcg tggcccactc cgcgatcgac gaggagaact
     1201 tggacgccgc cgtcgagcgt gagcgacgct ggctggatcg tccacgccgt ggtgcgctcg
     1261 tcgggaatcg tgctggtctc gaagccgatg cggccgccgc cgcgcacgaa ctgacccgtc
     1321 tcgacctgct cgatgccgcc ccacaatgcg tacgagtcgt gaaagcctcg cgcgcgcggc
     1381 atccactcgg ggattccttg cgccgcgaac gtcgaaccga acgcgcgcac gtcgtaggta
     1441 ctcatgcgcg agaggtcttc ccaacgcccg ccgatgtgga gatcgatatc gctcaccaac
     1501 agcttgcgga cctcgacatc gacgctcgcg ggttgcgaca gatacacggt ggatgcaccg
     1561 gtgatcgtat tgccgccatc acggggtgcg cgctcgacgg cgatcgtgcc ggtcagcgtg
     1621 ttctgaaccg cgagaccggg aggcaggtga tagctcgcgc cgagccagat atcgcgcggc
     1681 agcttgacga gcgtgccgat cgtcgcgccg atgacgttcg aggcagacaa gatgccggtg
     1741 ttgacgtcga cgtcgtagac ctcggtcgcg gacgcattct cgatgccgca tggcgagcct
     1801 tcgcagtcgc tgtcgatgcc gcgcgcggga tcgcgacccg cttcgagcgc ggtgtctcgc
     1861 gcgtagcgaa gtcgcagcca cgagatctgc gccgagagcc cgatgccgaa gtaccaccgg
     1921 ttcgtgatcc gaaagctcgc ggcgatgtcg gccgaacccg tgcggtgata cccgccgagg
     1981 atcgagtact gaagcgggtc gtgactctcg aggaatcgct ctgcgggcgc cgagcggaac
     2041 tgaccggcga gcgtgatctt gccgtcggtg ccggtgtgcc acaggccggc gagtgttccg
     2101 ccaggcgaga gcgtcgtgtc gctgacgtga ccggtcgtcg tgacgcctcg atgatcgatc
     2161 ttgaattgat cgacggcagc gagggccgcg atatagatct cgttcttcgc gccgacgccg
     2221 agcgctgcgg gattgagctc gatcgatgtc ggatgctgga ctgtcgcgcc ggtgaagacg
     2281 gtgcggccga tcgtcggatc gctgcgcggc gaggcgtgcg cacgtgctgc caacaggcag
     2341 agcgcaatgc aggcggcgcg agacatcgcg cggaagttat caccacgata agctgccgcc
     2401 atgctcgcgc gcctcgtacc gatcgccttt gcagccatgt tgctaacagc gggctgcgag
     2461 aagaccgatc acgccacgat cgataaatgg atggggacga agaaggggcc cagcaagctg
     2521 aagaagacgc tggtcgacga ctcgctcgat ccggatctct cggcgcacgc cgccgcgaac
     2581 atgatcaaga agggcaggga ccaagaggtc cgcacggagc tcgacaatat gtcggtgccg
     2641 cgcaagcagg cgatcgtcgg caagctcgcg ccgcggctgt gggagatggc gcgcatcgag
     2701 ggcgagatga tgatgccggc gccgcaacag gtgcaggcga aagacgcgct cgtctcgatc
     2761 cgcaaatacg ccgacacgcc gatgcgcacc acgatcgatg gctacttgat cgattggtac
     2821 acggtgccgt cgtacgaagg ccgctcgaag gcaggcgcgg tcctcggcga agcggtgatg
     2881 cgcatggtcg gtcccggcgc ggcgaagaag ctcatgcgcg tggccgacgg catcatcgcg
     2941 gcgcccggtc aggacaagac gaagaagcgc atcggcgatc agctgctgct cggtctcgcc
     3001 gcgaccggtg atcccgacgc ggtgaagtac atcctcgaca tcgcgaagat ggatcgcggc
     3061 gacgagaccc tcgcgaagcg cgcgctcgga gcgctctaca ccgcgtatgt cgatccccag
     3121 ggcctgttcg atctccagga gcccggtgcc ctggtgccga acctcgatca gctggtgtcg
     3181 atcgccaaga gcgactcgat ggaccctgca gcagccaact cggccgtcgc cctgatccgg
     3241 gtcgtcgggc ccccacagtg cctacagccg ctcgtctcga tgatcgctca cccacatcca
     3301 gacccgcgct tccgctactt cgggcccgac gcagcgctca agtgtggagg tgtaaaggcg
     3361 atcaagcagg tcatccaagc catgcccgac caggcctacg agaaggtgaa gctcgagggc
     3421 acggtcgtcc tcgacatcac gaagatgagc ccgcgcgacc aggtgctcgc cgaactgcgt
     3481 gatctcttga atgacaagag ccgcatggcg cggtgggtcg cgatcgagac cctcgctgcg
     3541 atgaagtcca aagaggacgc ccccgccatc gcgaaggtca aaggcgggga gaagctcctc
     3601 gggttctggg gggatcagag cgggacggac cccgcggctc gcaagtcgga ccccaccctg
     3661 gctcagcgcg caaaggaact gtcggacgcc ctccagaaag cgccctaacc cccgcccaac
     3721 taggcggctt cctcgcgtct tttccgggca tgtagtaagg tagtagatgc ccgggagagg
     3781 aaggcggcgt tcgtcgctcg gctaggagta ccccgcgaat ggatcagagc aaaaagacga
     3841 tgcggcactt ttactgccgc gacgtgcttt gggagacgtt cgagca
//
LOCUS       DATLBZ010000651         6029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3643119, whole genome shotgun sequence.
ACCESSION   DATLBZ010000651 DATLBZ010000000
VERSION     DATLBZ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643119"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VMZ53_31990"
     CDS             complement(<1..730)
                     /locus_tag="VMZ53_31990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HUS33182.1"
                     /translation="MAQSKTKSSKPAAKAPSKDKGKPAAKPSGKKDDEKDTKALATDK
                     AAKKLGAPAAKAAPAPKAAKAAKPAKGGKVSAPRDEDGDGFDGDGDDEEDDEFAPPAA
                     KAGKPGKPGAKAAKGPRPEDLVDIDDDVVAPIGLDDDEDEDIAGAPRRSLSADADAAA
                     MEAETRGKKGRGGNNANNAMTSAKDKLIELGKQKGFVTYDEVNDAMPEDVVSTDQIDS
                     WLSALGDHGIEVVDGAGGRRAGEIV"
     gene            complement(733..2706)
                     /gene="dnaG"
                     /locus_tag="VMZ53_31995"
     CDS             complement(733..2706)
                     /gene="dnaG"
                     /locus_tag="VMZ53_31995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HUS33183.1"
                     /translation="MIADEIINEIRSRADIVAVIGQHVQLRKAGRNWKGLCPFHGEKT
                     PSFNVSPDKGFFHCFGCQKHGDVFTFVMELEGKSFVEAAEQLGARFGVQVPKIDESPE
                     LRKARGERIAMLEINKHATAFFREVLADPKRGEPGRAYLAKRGVAAEVTAKFQLGYAP
                     PDWHALCDYLKAKKCDMELATKLGLVSRQPRAGGYYDRNRDRLVCPVIVTSGDVVGFS
                     SRVIGKPPAAPDGSEPPKYINSPESPVYKKSFLLFGIAQAREAIQKNGRAVLVEGNFD
                     VISLHQAGFTDVVAPLGTALTSEQVLTLKRLSERIVLLYDGDKAGYKATMHALQLCVE
                     ADVEVLVAARPGNAKSGGGGVLADGMDPDSLVAGGGAEQLREAIERARGGIEFFAFEV
                     WSKARASTDARARAIDEAAKLVTRIENPTKRDMVIHTTALALEIDPAVIRTAVGRAAQ
                     RPDPRRGPAPQGRQGGPPSDRHPNAPGGHPNAPANSPGNSSGFTVGADSRDRDKTPAR
                     SLDPEVEVFSLLADHPSLLTTPDADRVSSLLTEPLLQAMYSAARAGQSFLELASTMLP
                     PPTAQRVLSGKYAESKDPSAQLRALLGSLDQRASMLAQNKLEKSLSQSKRGGMDPDFV
                     RLQTQLAVAQRKGDRELVEQLMEQISSNRKQAD"
     gene            complement(2793..3029)
                     /locus_tag="VMZ53_32000"
     CDS             complement(2793..3029)
                     /locus_tag="VMZ53_32000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33184.1"
                     /translation="MSDSGSTELGKEFPGSERRKHRHDLGHEDHGPKIAAGTTRGIQI
                     LLGLWIALTLVFVVYALVDASRPKAEIGDAVSDD"
     gene            complement(3070..3885)
                     /locus_tag="VMZ53_32005"
     CDS             complement(3070..3885)
                     /locus_tag="VMZ53_32005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33185.1"
                     /translation="MKRAFAVVVVLGSACSGGSNGPDAPVDDNLTRPDATDDQDHDTI
                     LDKDDNCPSVVNLNQGNEDGDKFGDACDPCPIVADDDPPDTDGDKVADACDPLPTMPG
                     DFIRYFEGFHTAVPAGWEEIGVWTHTTGAVRTTSAAAGLAIIATDRTRETISAALTFD
                     AVTGTSRAGVVDTKMAGGPGVACVIAADPSVEVYATNNAGNATQTSYEQTTNTKYVVK
                     LRRNMNEYTCTASAGATASAMKTLTLTPTPYLSGITAADSTVRFHWFMVVESL"
     gene            complement(3882..5222)
                     /locus_tag="VMZ53_32010"
     CDS             complement(3882..5222)
                     /locus_tag="VMZ53_32010"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33186.1"
                     /translation="MPRLGELLTSTRLIEPEKVEQALRAQVMWGGRLGTNLIELGCID
                     LEGLSRALGRQHGLPAALARHFDKADPELQKKLPAELARKWSVVPLLYVGADKKIALA
                     ALDPLPPEGLVAIADAFGVPERVLVVSIAAEMRVRYNLERFYGVARPARFLRAKGKTI
                     TPFPQFEEFDVETDAELIAQSAVPEVIVAKPTGRAAVPPPPVNADDLTALIDAAIENA
                     QAVEPDDAPKGRERRTYVKTLGEVEPSLPPAPTITSAFPAVSPPDEDEFDAKPPTLGR
                     MALKRVVIANEPTTLHEAAKAIRRGITRDRVAELVIAALERFVPKCDAALLLVIRGDI
                     ATGWVHFSRDGSSTPDIAVPLDKPGLVPRSIMHNMTTRGMPDDLNAIDQALAASLGDA
                     DPEDHLAISPISIGGQVMCLLAAVIKADAPSSPLDVVASAAGAAFTRLIRDASR"
     gene            complement(5235..>6029)
                     /locus_tag="VMZ53_32015"
     CDS             complement(5235..>6029)
                     /locus_tag="VMZ53_32015"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HUS33187.1"
                     /translation="MMFDPEHIVRLALEGGCSAVASTFGVLGLVARKFAHKIPFLVKL
                     NHNELLSYPNTFDQTLFGSVRQCFDMGAQGVGATVYWGAAESRRQLHEIAEAFAEAHR
                     LGMFTVLWCYVRNSAFKTAQGDHSLSADLTGQANHLGVTIQADIIKQKLPELNGGYND
                     PALKGYGKTHKKVYSELTTENPIDLTRYQVANCYMGRVGLINSGGASSGESDLREAVT
                     TAVINKRAGGMGLIAGRKAFQRPFKEGVDLLNAIQDIYLCKDVTVA"
BASE COUNT          911 a         1974 c         1995 g         1149 t
ORIGIN      
        1 cgacgatctc gcctgcgcgg cggccgcctg caccatcgac gacttcgatg ccgtgatcgc
       61 cgagcgccga cagccagctg tcgatctgat cggtggagac gacgtcctcg ggcatcgcgt
      121 cgttgacctc gtcatacgtg acgaagccct tctgcttgcc gagctcgatg agcttgtcct
      181 tcgcggacgt catcgcgttg ttcgcgttgt tgccgccgcg gcccttcttg ccgcgcgtct
      241 cggcttccat cgctgctgca tcggcgtcgg ccgagaggct gcggcgcggt gcgcctgcga
      301 tgtcttcgtc ttcgtcgtcg tcgaggccaa tcggcgcaac gacgtcgtcg tcgatatcga
      361 cgaggtcttc gggacgcggg cccttcgcgg ccttcgcacc cggcttgccc ggcttgccgg
      421 ccttcgcggc aggcggagcg aactcgtcgt cctcttcgtc gtcaccgtca ccgtcgaagc
      481 cgtcgccgtc ttcgtcgcgc ggagcagaga ccttgccacc cttggccggc ttggcagcct
      541 tcgccgcctt cggagccggg gccgctttcg ccgccggagc accgagcttc ttcgctgcct
      601 tgtcggtagc gagtgctttg gtgtccttct cgtcgtcctt cttgccggac ggcttcgccg
      661 caggtttccc cttgtccttc gacggtgcct tggccgctgg cttactgctc ttcgtcttcg
      721 actgcgccat cttcaatctg cctgctttcg gttggacgag atctgctcca tgagttgttc
      781 gaccaactca cggtcacctt tacgctgcgc aactgcgagt tgcgtttgaa gtcggacgaa
      841 atcgggatcc atgccaccgc gcttggactg cgagagactt ttctcgagct tgttttgcgc
      901 gagcatcgac gcacgctggt ctagtgaccc gaggagcgcg cgaagttgcg cggacggatc
      961 tttagactcg gcgtattttc cggacagcac gcgctgagcg gtgggcgggg ggagcattgt
     1021 tgatgcaagc tccaggaagg actgtcccgc ccgggccgcg gaatacatgg cttgaagcag
     1081 gggctccgtc aagagggaag aaaccctgtc tgcatctggg gttgtgagca aactcgggtg
     1141 gtccgcgagt agggaaaaga cctcgacctc gggatccagg ctccgagcgg gagttttgtc
     1201 acgatctcgc gagtctgcgc cgaccgtgaa gcccgacgaa ttcccgggcg aattcgcagg
     1261 ggcattgggg tgaccgcccg gcgcgttcgg gtggcgatcg gatggcggac cgccctgtcg
     1321 accctgcggc gccggtcctc gacggggatc tggccgctgc gccgctcgtc cgaccgccgt
     1381 acgaatcacg gccggatcga tctcgagcgc gagcgccgtc gtgtgaatca ccatgtcgcg
     1441 cttcgtcggg ttttcgatcc gcgtgacgag cttcgcggct tcgtcgatcg cacgcgcccg
     1501 cgcatccgtg ctcgcgcgcg ccttgctcca cacctcgaac gcgaagaact cgatgccgcc
     1561 gcgtgcgcgt tcgatcgctt cgcgcagctg ctcggcgcca ccgcccgcga cgaggctgtc
     1621 gggatccatg ccgtcggcaa gcacgccacc gccgcccgac ttcgcattgc ccggacgcgc
     1681 cgcgacgagc acctcgacgt ctgcttcaac gcagagctgc agtgcatgca tcgtggcttt
     1741 gtagccagct ttgtcgccgt cgtagagcaa gacgatccgt tcggagaggc gctttagcgt
     1801 cagcacctgt tcgctcgtga gtgccgtccc cagcggcgcg accacgtcgg taaatccggc
     1861 ctggtggagc gaaatgacgt cgaagtttcc ttcaactaag accgcgcgcc catttttttg
     1921 tatcgcctcg cgcgcctggg cgatcccgaa cagcagaaag ctcttcttgt agaccgggct
     1981 ctcggggctg ttgatgtact tcggcggctc gctgccgtcg ggcgcggcgg gtggcttgcc
     2041 gatgacacgc gacgagaacc cgacgacgtc gccgctcgtc acgatgacgg gacagacgag
     2101 ccgatcgcga ttccgatcgt agtagccgcc cgcgcgaggt tgcctcgaca cgaggccgag
     2161 ctttgtcgcg agctccatgt cgcatttctt ggccttcagg tagtcgcaga gcgcgtgcca
     2221 gtcgggcggc gcatagccga gctggaactt cgccgtgacc tcggccgcga cgccgcgctt
     2281 cgcgagatac gcacgacctg gctcgccgcg cttgggatcc gcgagcacct cgcgaaagaa
     2341 cgccgtcgcg tgcttgttga tctcgagcat cgcgatgcgc tccccgcgcg ccttccgcag
     2401 ctcgggcgac tcgtcgatct tcggcacctg cacgccaaac cgcgcgccga gctgctcggc
     2461 cgcttcgacg aagctcttac cctcgagctc catcacgaac gtgaacacgt cgccgtgctt
     2521 ctggcagccg aagcaatgaa agaacccctt gtcgggcgac acgttgaacg aaggcgtctt
     2581 ctcgccgtga aagggacaaa gccccttcca attgcgcccc gccttgcgca gttgaacgtg
     2641 ctgtccaatg accgcgacga tgtccgcgcg actgcggatc tcgttgatga tctcgtcggc
     2701 aatcatgacc actaacccga gtctccgcgc cgcccccgac gcgaatcagg gcaccttgac
     2761 ccattcctcc ccagttgacc aggcgtaacc gatcaatcgt cgctgacagc gtcaccgatc
     2821 tcggcctttg gccgagatgc gtcgacgagt gcgtacacca cgaacacgag cgttagcgcg
     2881 atccacagac cgaggaggat ctggatgccg cgcgtcgtac ccgcggcgat cttcggacca
     2941 tggtcttcat ggccgaggtc gtgccggtgc ttcctgcgct ccgaacccgg aaactctttg
     3001 cccagctcgg tcgatccgct gtcgctcacg cccggaccat gccttcgggg gctgacaaat
     3061 tcaaggccgc tacaggctct cgacgaccat gaaccagtgg aacctcacgg tcgaatccgc
     3121 ggccgtgatt cccgagagat agggcgtggg cgtgagcgtg agcgtcttca tcgcgctcgc
     3181 ggtcgcgccg gcggatgccg tgcacgtgta ctcgttcatg ttgcggcgca gcttcacgac
     3241 gtacttcgtg ttcgtggtct gctcgtagct cgtctgcgtc gcgttccctg cgttgttcgt
     3301 cgcgtagacc tcgacggacg gatcggcggc gatcacgcat gcgacgccgg gaccacctgc
     3361 catcttcgtg tcgacgacgc ccgcgcgcga cgtgccggtc accgcgtcga aggtgagcgc
     3421 ggcggagatg gtctcgcgcg tcctgtccgt cgcgatgatc gcgaggcccg cggcggcgga
     3481 ggtcgtgcgc acggcgccgg tcgtgtgggt ccagacgccg atctcttccc agcctgcggg
     3541 caccgccgtg tgaaagcctt cgaagtagcg gatgaagtcg cccggcatcg tcggcagcgg
     3601 atcgcaggcg tccgcgactt tgtcgccgtc ggtgtcgggc gggtcgtcgt cggcgacgat
     3661 cgggcatggg tcgcacgcgt cgccgaactt gtcgccgtcc tcgttgcctt ggttgaggtt
     3721 cacgacgctc gggcagttgt cgtctttgtc gaggatcgtg tcgtgatctt gatcgtcggt
     3781 ggcgtcgggg cgcgtgaggt tgtcatcgac gggcgcgtcg gggccattcg agccgccgct
     3841 gcatgcggag ccgagcacga cgacgacggc gaacgcgcgc ttcatcgcga tgcatcacga
     3901 atgagtcgag tgaacgcagc gccggcggca ctcgcgacga catcgagcgg cgacgacggg
     3961 gcatccgcct tgatcaccgc ggcgagcagg cacatgactt ggccgccgat cgagatcgga
     4021 ctgatcgcga gatgatcctc gggatccgcg tcgccgagcg aggcggcgag cgcctgatcg
     4081 atcgcgttga gatcgtcggg catcccgcgc gtcgtcatgt tgtgcatgat cgaccgaggc
     4141 acgagccccg gcttgtcgag gggcaccgcg atgtccggtg tcgacgagcc atcgcgcgag
     4201 aagtggaccc agccggtcgc gatgtcgccg cggatcacga gcagcagagc ggcatcgcat
     4261 ttcggcacga accgctcgag cgcggcgatg acgagctcgg ccacgcgatc gcgtgtgatg
     4321 ccgcggcgga tcgctttcgc ggcttcgtgc agcgtcgtcg gctcgtttgc gatgacgacg
     4381 cgcttgagcg ccatgcgtcc gagcgtcggc ggtttggcgt cgaactcgtc ctcgtcgggc
     4441 gggctgaccg cggggaacgc cgacgtgatc gtcggagcag gcggcaggct cggttcgacc
     4501 tcgccgagcg tcttgacgta cgtgcggcgc tcgcggcctt tcggtgcgtc gtccggctcg
     4561 acggcctgcg cattctcgat cgccgcatcg atcagcgcgg tgagatcgtc ggcgttgacc
     4621 ggtggaggcg gaaccgcggc gcgacccgtc ggcttcgcga cgatgacctc gggcaccgcc
     4681 gattgcgcga tcagctccgc atcggtctcg acatcgaact cttcgaactg cggaaacggc
     4741 gtgatcgtct tgcccttcgc gcgcagaaag cgagcggggc gagcgacgcc gtagaagcgt
     4801 tcgaggttgt agcgaacgcg catctcggcc gcgatcgaga cgacgagcac tctctctgga
     4861 acgccgaacg catcggcgat cgcgacgaga ccttccggcg gaagcggatc gagtgcagcg
     4921 agcgcgatct tcttatcggc gccgacgtag agcagcggca cgaccgacca cttgcgcgcg
     4981 agctccgcgg gcagcttctt ctgcagctcg ggatccgctt tgtcgaagtg gcgcgcgagc
     5041 gcagcgggaa ggccgtgctg gcgaccgagc gcgcgcgaca gtccttcgag atcgatacag
     5101 ccgagctcga tgaggttcgt gccgaggcgg ccgccccaca tgacctgcgc ccgcagcgcc
     5161 tgctcgacct tctcgggttc gatcagacgc gtggacgtca gtagctcgcc cagccgtggc
     5221 attcagccga gtatctacgc gactgtgacg tctttacaga ggtagatgtc ctggatcgcg
     5281 ttgaggaggt cgacaccttc cttgaacgga cgctggaatg ccttgcggcc ggcgatcaga
     5341 cccatgccgc cggcgcgctt gttgatgacg gccgtcgtga ccgcctcacg gagatcgctc
     5401 tcaccgctcg acgcaccgcc cgagttgatg aggcctacgc ggcccatgta gcagttcgcg
     5461 acctggtaac gggtcaggtc gatcgggttc tcggtcgtca gctccgagta gaccttcttg
     5521 tgggtcttgc catagccctt gagcgccggg tcgttgtagc cgccgttcag ctcggggagc
     5581 ttctgcttga tgatgtccgc ctggatggtc acgccgaggt ggttggcctg gcccgtgagg
     5641 tcggccgaca ggctgtgatc accctgcgcg gtcttgaacg cgctgttgcg gacgtagcac
     5701 cacagcaccg tgaacatgcc gaggcgatgg gcctcggcga acgcctcggc gatctcgtgc
     5761 agctggcggc gcgactcggc ggcgccccag tagacggtcg cgccgacgcc ctgcgcgccc
     5821 atgtcgaagc actggcgcac gctgccgaac agcgtctggt cgaacgtgtt cgggtacgac
     5881 aggagctcgt tgtggttgag cttcacgagg aacgggatct tgtgcgcgaa ctttcgcgcg
     5941 acgagcccga gcacgccgaa cgtcgacgcg acggcgctgc agccgccctc gagcgcgagc
     6001 cgcacgatgt gctctgggtc gaacatcat
//
LOCUS       DATLBZ010000652         6612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7955676, whole genome shotgun sequence.
ACCESSION   DATLBZ010000652 DATLBZ010000000
VERSION     DATLBZ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7955676"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VMZ53_32020"
     CDS             <1..469
                     /locus_tag="VMZ53_32020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33188.1"
                     /translation="ISALFTKLALDERAAGIIYFDAADWGAPASALAPAIADAPVADR
                     GLDGVFAPYFWDVPYADAAFAEIQALRERGITTGCATAPPRFCPDEPLRAADAAALVA
                     AAFPTAQPSFSEPMTELDLANALVALGASPPPAAPVALTRRRAAVLIAHALLD"
     gene            complement(509..1357)
                     /locus_tag="VMZ53_32025"
     CDS             complement(509..1357)
                     /locus_tag="VMZ53_32025"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HUS33189.1"
                     /translation="MSVVLGIDAAWTANQPSGVALVRSHGRAWQCLALAPSYESFITL
                     AAARPARTGVRPAGTSTRSPAASMPLDWRRRPQGGPPDCDALLAAASALAGAAPDVIA
                     IDMPLATRRITGRRVADNALSSAYAARGLGVHTPNEQRPGPIADAMRLGFGRHGYKVA
                     TARTPPATPRVLIEAFPHAAAIVLAGANYRVPYKLSRIAQYWPSSEPAARRRALMAQW
                     RVLRRALARTIDGGELRIPSGGPLAALKRYEDALDALICAWIAIEYLAGRLRAHGDAT
                     AAVWAH"
     gene            1380..2276
                     /locus_tag="VMZ53_32030"
     CDS             1380..2276
                     /locus_tag="VMZ53_32030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33190.1"
                     /translation="MRSLLWIVVALGAAGCQQVLGLEPPVGGGGDAGTTDSFVVDAAP
                     DGAASVDSDSDGVNDALDNCRQVPNANQRNEDNDSLGNACDPCPISSNNADVDADGVA
                     DACDPHPAIGGDVLRLFDSFQDGIPGTWSVDGGTWTSDNGDIVGGSVNNVQGDLYLPS
                     AYSTKETITIGTTVTSASGTSFRIVGVKDNAAIDGFAVVCSMIITPLADVPSNTPMND
                     LYEQPASTAYERVPFTWLLNTPMTIRLQRANNVYTCAGTQGSSSTTTNANENTSTNGA
                     RIALHVQSLTARFHWLMVVSSP"
     gene            2273..3247
                     /locus_tag="VMZ53_32035"
     CDS             2273..3247
                     /locus_tag="VMZ53_32035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33191.1"
                     /translation="MKRIALVVVAACGSSTATPHETETIARTVPSGPSAREVEARRER
                     EHRAEVTAAHRKLEDAQQEAMSAACEDPKPASTHERCLPSCYTTEAPDPRAAKKPKGA
                     LEIERIVCEADDRAFVVDELDATLAAKPKRGRLKPHKKGSWQADLEAKLRDEQFAKGS
                     IVVVTGAWKSMTHPLTKTKLKCVAVSQFASAAKPLDACGTLGEAACEAMGNGAARAIN
                     VVHYRLAEAKALQAAGKQDDCQRASLEAVAVARGLPRWRQYMKLNEAKWDKRERYRTR
                     FDGFLDEEALFSLVQTMGAEAESVHSACGGAPNAPTTAEQEQSFHTCW"
     gene            3257..3529
                     /locus_tag="VMZ53_32040"
     CDS             3257..3529
                     /locus_tag="VMZ53_32040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33192.1"
                     /translation="MVHREEAIWTIRIEASAEFDEQYDGELDGFVWREAQFRELQNAV
                     IGAVMQKLQRMPGWRVRTGNRGLPATDEVLVHAELDVDSEAHQSKR"
     gene            complement(3514..5127)
                     /locus_tag="VMZ53_32045"
     CDS             complement(3514..5127)
                     /locus_tag="VMZ53_32045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS33193.1"
                     /translation="MADPQARAAPLPAKIGRHAVVGYIATGGMAELYLGKEPTGRPVV
                     IKRILPHLARQTSFVSMFIDEARIGSRAKHPNLVEVHELGQVGTDLFLVMEYLVGENL
                     AGLVRRLIKRGERISYGLCAYLIAEVCDGLHAAHELTDDAGNHLELVHRDVSPQNVFV
                     TYGGDVKLLDFGVATAAHRLTQTASGEVKGKYAYMSPEQCRGEPLDRRSDIFSLGIVL
                     YELTTLRRLFKRANELQVMKAITEDPIPRPTREAPDYPQALEDICVRALARDPAQRYA
                     TAAEMREDLLRAMQQIGLDGDPHEAIASKLARLFADRMIQKRELMERVRGGGDLTAYL
                     VPEVDEQVEVPLVSAVEATPLSRVRGAAAQPPKKKRGVAFLVAFLTAVICGAGVGAYL
                     RLHRKPAAPVAKKQPPPAPIVATAMPDAAPHVEMEIEDVEEVPPAPPEQEYIVVSVET
                     DPAGVKVKLDGEERGATPIDVKIKKDTTPLKLELMSPGFTTAAQQIVPDRDQRMYFSL
                     IKTEKTIVRIKRPKTRPKNDRGSGFHRFD"
     gene            5151..5963
                     /locus_tag="VMZ53_32050"
     CDS             5151..5963
                     /locus_tag="VMZ53_32050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS33194.1"
                     /translation="MLRVSYVFAIALALVGPAYADDNRGKALKLFEESNKAYKSGKFE
                     DAADLLKKAYALFPEPILMYNLGRAQEGLGDPKGALDSYERYLAEAKQVDDRGAIERR
                     IATLKAQIAKQEEDARSRDENERRLQEEQERRKHEEQERQRLEAERQRLEDERARAVV
                     IDDRTPLEKYGPWTTMGVGGALVGTGIVFGIRSGAKHDDAVAAPGQRDAADLQASAEH
                     YATVANVLFVAGGLVAAGGVAWKVWQWKHPHAQTAASLQVTPTSAAVEWVFP"
     gene            5960..>6612
                     /locus_tag="VMZ53_32055"
     CDS             5960..>6612
                     /locus_tag="VMZ53_32055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33195.1"
                     /translation="MRPALLAALAMLLVELAGCSVPAVSLEGKQCPCAEGFVCDDLTK
                     RCLATNDGGTIIDTPAATSCLSESTSTIYQYAGAFDWQHEDPSWMGGAQITQTATNAM
                     NSYAYRTSAEFTTKKDYRVIATMRQTAAGMGTPSLGIVLRAQLSPQDKSRYACVWIPK
                     NKELRIEATHGGSTATLGSALVANTGATMSYTMEARVTGAAPPQLSCCIRELPAAKIA
                     "
BASE COUNT         1139 a         2213 c         2246 g         1014 t
ORIGIN      
        1 gatctcggcg ttgttcacga agctcgctct cgacgagcgc gcggccggca tcatctactt
       61 cgacgccgcg gactggggag caccggcgag cgcgctcgca cccgcgatcg ccgatgctcc
      121 ggtcgccgat cgcggtctcg acggcgtgtt cgcgccctac ttctgggacg tgccgtacgc
      181 ggacgcagcg ttcgccgaga tccaggcgct gcgcgagcgc ggcatcacga cgggctgcgc
      241 gaccgcgcca ccacgcttct gtcccgacga gccactccgc gctgccgacg cagccgcgct
      301 tgtcgcggcc gcgttcccca cggcacagcc gtcattcagc gaaccgatga ccgagctcga
      361 tctcgcgaat gcgcttgtcg cgctcggcgc gtcgccacca ccggcagcgc ccgttgcgct
      421 cacgcgtcgt cgagccgcgg tgctcatcgc ccacgccctg ctcgactgaa acgtcgtcgc
      481 ggagccgtcc tcatcacggc cggttcgatt agtgcgccca caccgcagcc gtcgcatcgc
      541 cgtgcgcgcg gaggcgaccc gcgaggtact cgattgcgat ccacgcgcag atcagcgcgt
      601 cgagggcgtc ctcgtagcgc ttcagcgcgg cgagcgggcc gcctgacgga atgcgcagct
      661 cgccaccatc gatcgttcgc gcgagcgcgc gacgcaacac gcgccactgc gccatcaacg
      721 cgcggcgccg cgccgccggc tccgacgacg gccagtactg cgcgatccgc gacagcttgt
      781 agggcactcg ataattcgcg cccgcgagca cgattgcagc ggcatgtggg aaggcctcga
      841 tcagcacgcg cggtgtggcg ggcggcgttc gcgcagttgc gaccttgtag ccgtgccggc
      901 cgaagccgag gcgcatcgcg tcggcgatcg ggcccggccg ctgctcgttc ggcgtgtgca
      961 cgccgaggcc acgcgcggcg tatgcggacg agagtgcgtt gtcggcgacg cgacgccccg
     1021 tgatgcgacg tgtggcgagc ggcatgtcga tcgcaatgac atcgggtgcc gcaccggcga
     1081 gcgcagacgc cgccgcgagc agcgcatcac agtcgggcgg gccaccttgc gggcgccggc
     1141 gccagtcgag cggcatcgac gctgcgggtg accgtgtcga cgtgcccgct gggcgaacgc
     1201 ccgtgcgtgc gggacgagcg gcggcgagcg tgatgaagct ctcgtagctc ggcgcgagcg
     1261 cgaggcattg ccatgcgcgg ccgtgcgagc ggaccagcgc gacaccgctc ggttggttag
     1321 cggtccacgc ggcgtcgatt ccgaggacaa cgctcacgtg gatcgaatac cctgaattgg
     1381 tgcggtcgct gctgtggatc gtcgttgcgc tcggggcagc gggctgccag caggtgctcg
     1441 gcctcgaacc gcccgtcggc ggcggcggcg acgcaggcac gaccgactcg ttcgttgtcg
     1501 atgccgcgcc tgacggcgct gcgagcgtcg acagtgatag cgacggcgtg aatgacgcac
     1561 tcgacaattg ccgccaggtc ccgaacgcga accagcgcaa cgaggacaac gactcgcttg
     1621 gcaatgcctg tgatccctgc cctatctcgt cgaacaacgc ggacgtcgat gcagatggcg
     1681 tcgccgacgc gtgtgatccg cacccggcga tcggcggcga tgtcctgagg ctcttcgact
     1741 cgtttcagga cggcatcccc ggaacgtgga gcgtcgacgg cgggacctgg acttctgaca
     1801 acggtgacat cgtcggcggc agcgtcaaca acgtgcaagg cgatctgtat ttgccgtccg
     1861 catactcgac gaaggagacg atcacgatcg gcacgacggt cacgagcgcc agcggcacca
     1921 gttttcgcat cgtcggcgtg aaggacaacg cagcgatcga tggcttcgcc gtcgtgtgct
     1981 ctatgatcat cacaccgctt gccgacgtgc cgtcgaacac gccgatgaac gatctctacg
     2041 agcagcctgc gagcacagcc tacgagcgcg tcccgttcac ctggctgctc aacacaccga
     2101 tgaccatccg gctccagcgg gcgaacaacg tgtatacgtg cgccggcacg cagggctcca
     2161 gctcgacgac gacgaacgca aacgagaaca cgagcacgaa tggtgcgcgc atcgcgctgc
     2221 acgtccagtc cttgaccgcg cgctttcact ggctgatggt cgtctcgagc ccatgaaacg
     2281 catcgcgctt gtcgttgtcg ccgcgtgcgg ctcgagcacg gccacaccac acgagaccga
     2341 gacgatcgcg aggacggtgc cgagcgggcc gagcgcgcga gaggtcgagg cgcgccgcga
     2401 gcgcgagcac cgcgcggagg tcaccgcggc gcatcgcaag ctcgaggacg cgcagcaaga
     2461 ggcgatgtcg gccgcgtgcg aggatccgaa acctgcgagc acgcacgagc gctgcttgcc
     2521 gagctgctac acgacggagg cgccggaccc gcgcgccgcg aagaagccca agggcgcgct
     2581 cgagatcgaa cgcatcgtgt gcgaggccga tgaccgcgcg tttgtcgtcg acgagctcga
     2641 cgcgacgctc gctgccaagc cgaagcgtgg ccgcctaaag ccgcacaaga agggcagctg
     2701 gcaagccgac ctcgaggcga agctccgcga cgagcagttt gcgaagggct cgatcgtcgt
     2761 cgtgaccggc gcgtggaagt cgatgacaca tccgctgacg aagacgaagc tcaagtgcgt
     2821 cgcggtctcg cagttcgcgt ccgcagcgaa accgctcgat gcatgcggca cgctcggcga
     2881 agcggcgtgc gaggcgatgg gcaacggcgc ggcgcgcgcg atcaacgtcg tgcactaccg
     2941 gctcgccgaa gcgaaggcgc tccaagctgc gggcaagcag gacgactgcc agcgcgcgtc
     3001 actagaagcg gtggcggtcg cgcgcgggct accgcggtgg cgtcaataca tgaagctcaa
     3061 cgaagccaag tgggacaagc gcgaacgcta ccgcacgcgg ttcgatggat tcctcgacga
     3121 ggaagcgttg ttctcgctcg tgcagacgat gggcgccgag gccgagagcg tgcacagcgc
     3181 ttgcggcgga gcgccgaacg caccgacgac agcagagcaa gagcagtcgt tccacacctg
     3241 ctggtagtct gccggcatgg ttcatcgcga agaagcgatc tggacgatcc gcatcgaagc
     3301 cagcgcggag ttcgacgagc agtacgacgg cgagctcgac ggcttcgtgt ggcgtgaggc
     3361 gcaatttcgc gagctgcaga acgcggtcat cggcgcggtg atgcaaaaac tccagcgcat
     3421 gcccggctgg cgcgttcgca ccggcaatcg tgggctaccg gcgaccgacg aggtcctcgt
     3481 ccacgccgag ctcgacgtcg attccgaagc gcatcaatcg aagcggtgaa aaccgctgcc
     3541 gcgatcgttc ttgggccgcg tcttcggccg cttgatgcgc acgatcgtct tctccgtctt
     3601 gatcagcgag aagtacatcc gctgatcgcg atccggaacg atctgctgcg cggccgtcgt
     3661 aaagcccggt gacatcagct cgagcttgag cggcgtcgtg tccttcttga tcttcacgtc
     3721 gatcggcgtc gcgccgcgct cttccccgtc gagctttacc ttcacgcccg cgggatccgt
     3781 ctcgacgctg acgacgatgt actcctgctc gggtggcgcg ggcggcacct cttcgacgtc
     3841 ttcgatctcc atctcgacgt ggggcgcggc atcgggcatc gccgtggcga cgatcggagc
     3901 gggcggtggc tgcttcttcg cgaccggtgc ggctggcttg cgatgcagtc gcagatacgc
     3961 gccgacacct gctccacaga tcaccgccgt gagaaatgcg acgagaaatg cgacaccgcg
     4021 cttcttcttc ggcggctgcg cagccgctcc gcgaacccgc gatagcggcg tcgcttcgac
     4081 cgcgctgacg agcggaactt cgacttgctc gtcgacctcg ggcacgagat acgccgtcag
     4141 atcgccgcct ccgcgcacgc gctccatcag ttcgcgcttc tggatcatgc gatcggcgaa
     4201 caggcgcgcg agcttcgatg cgatcgcttc gtgcggatca ccgtcgaggc cgatctgctg
     4261 catcgcacgc agcaggtctt cgcgcatctc ggccgcggtc gcgtagcgct gcgctggatc
     4321 gcgggcgagt gctcgcacgc agatgtcctc gagcgcttgc ggatagtccg gtgcttcgcg
     4381 cgtcggccga ggaatcggat cctcggtgat cgccttcatc acctgcagct cgttcgcgcg
     4441 cttgaagagg cgccgcagcg tcgtgagctc gtagaggacg atgccgagcg agaagatgtc
     4501 gctgcgccga tcgagcggct cgcctcgaca ctgctcggga gacatgtacg cgtacttgcc
     4561 cttcacctcg ccgctcgcgg tctgcgtcag gcgatgcgcg gcggtcgcga cgccgaagtc
     4621 gagcagcttc acgtcgccgc cgtacgtgac gaacacgttt tgcggcgaga cgtcgcggtg
     4681 gacgagctcg aggtgattgc ccgcgtcgtc ggtgagctcg tgcgcggcat gcaggccgtc
     4741 gcagacctcc gcgatcagat acgcgcacag cccgtacgag atgcgctcgc cgcgcttgat
     4801 gagccgccgc accaagccgg cgaggttctc gccgacgaga tactccatga cgaggaagag
     4861 atcggtgccg acttggccga gctcgtggac ctcgacgagg tttggatgct tcgcccgcga
     4921 gccgatgcgc gcctcgtcga tgaacatcga gacaaagctc gtctggcgcg cgaggtgcgg
     4981 caggatccgc ttgatgacga ccggccgccc cgtcggctcc ttgccgagat acagctccgc
     5041 catgccgccg gtcgcgatgt aaccgacgac cgcgtgccgc ccgatcttcg ccggaagggg
     5101 tgcggctctg gcctgcgggt cggccatgtt aggctcgatt atagctagcg atgctgcggg
     5161 tttcctatgt tttcgcgatc gcgctcgccc tcgtcgggcc tgcgtacgcg gacgacaacc
     5221 gcggtaaggc gctgaagctc ttcgaagaga gcaacaaggc ctacaagtcc ggcaagttcg
     5281 aggacgcggc cgacctcttg aagaaagcct acgcgctctt cccggagcca atcttgatgt
     5341 acaacctcgg ccgcgcgcag gaaggtctcg gcgatccgaa gggcgccctg gactcctacg
     5401 agcgctactt ggcggaagcg aagcaagtcg acgatcgcgg cgcgatcgaa cggcgcatcg
     5461 cgacgctcaa ggcacagatc gcgaagcaag aagaagatgc gcgctcgcgc gacgagaacg
     5521 agcgccgcct ccaagaagag caagaacgac gcaagcacga agagcaggag cggcaacgcc
     5581 tcgaagccga gcgccagcgt ctcgaagacg agcgcgcccg cgccgtcgtg atcgacgatc
     5641 gcacgccgct cgagaagtac ggcccatgga cgacgatggg tgtcggcggc gcgctcgtcg
     5701 gtaccggcat cgtgttcggc attcgctcgg gcgcgaagca cgacgacgcc gtcgcggctc
     5761 ccggccagcg cgatgcggcg gatttgcagg cgagcgccga gcactacgcg acggtcgcga
     5821 acgtgctgtt cgtagccggt ggcctcgtcg cggcgggcgg cgtcgcgtgg aaagtctggc
     5881 agtggaagca tccccacgcg cagaccgctg cctcgctgca ggtcacgccg acgagcgctg
     5941 ccgtcgagtg ggtgttcccg tgaggcccgc gctgctcgcc gcgctcgcca tgctgctcgt
     6001 cgagctggcc ggctgctcgg tgccagcggt ctcgctcgag ggcaagcaat gcccgtgcgc
     6061 cgaaggcttt gtctgcgatg atctcaccaa gcgctgcctc gccacgaacg acggcggcac
     6121 gatcatcgat acgccggcgg cgacctcgtg cctcagcgag tccacgtcga cgatctatca
     6181 gtacgccggc gcgttcgact ggcagcacga agacccgagc tggatgggcg gcgcgcagat
     6241 cacgcagacc gctaccaacg cgatgaacag ctacgcgtat cgcacgagcg ccgaattcac
     6301 gacgaagaag gactatcgcg tgatcgcgac gatgcgccag acggccgcgg ggatgggaac
     6361 gccgtcgctc ggcatcgtgc tgcgcgcgca gctcagcccg caggacaagt cgcgctacgc
     6421 gtgcgtgtgg atcccgaaga acaaagagct gcgcatcgaa gccacgcacg gcggcagcac
     6481 ggccacgctc ggctccgcgc tcgtcgcgaa tacgggcgcg acgatgtcgt acacgatgga
     6541 agcgcgcgtg accggcgctg cgccgccgca gctgtcgtgc tgcattcgcg agctgcccgc
     6601 ggcgaagatc gc
//
LOCUS       DATLBZ010000653        19129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3652926, whole genome shotgun sequence.
ACCESSION   DATLBZ010000653 DATLBZ010000000
VERSION     DATLBZ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19129
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3652926"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(530..1906)
                     /locus_tag="VMZ53_32060"
     CDS             complement(530..1906)
                     /locus_tag="VMZ53_32060"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HUS33196.1"
                     /translation="MKRLCLLAILVGCAADPVTETESVTEQEATVCGVGPVVKGIDVS
                     YYQGTINWTSVKNDGVKFAFIRVSDGLNTIDSKFAANWSGSRAAGVKHGAYQFFRPGQ
                     DPIEQADLLLQKIGGKLEADDLPPVIDVEASDGQSAAVITAKVKKWVAHVKAAIGRDP
                     IIYTGFYFWRDSVGAPDVTSSPLWHAQYSDAACPNIAPPWSKWALWQYSSTGTVAGIS
                     GNVDMNRWNGDMASLTAFLGPAATCGNSTCESGEGSLDCPEDCGPCTTIGKAGAVIDD
                     GDSCFLGGGPAASMRHVATAGLDGDLVWTHTTEDTAEGNYGTWHLWFEEAGLYKVEVY
                     TDGGFAQSKKAKYTVEAGAASKDVVIDQTAVDGWQSLGEFQFEAGAYQTIHLGDNTGE
                     LLADNVQMVFDGVRVTRVDPGTGSGDDQLDPPGDESSGCNARGGSGTGAGLLLALGLV
                     LRRRRRRR"
     gene            complement(2003..3088)
                     /gene="dmeF"
                     /locus_tag="VMZ53_32065"
     CDS             complement(2003..3088)
                     /gene="dmeF"
                     /locus_tag="VMZ53_32065"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HUS33197.1"
                     /translation="MATSHDLSAHAHHEEQGEHNRSGELRTLAVVGITALMMVGELIV
                     GHITNSTALKADGWHMGTHVGALGFTLIAYWYARRHAGNDAFSFGTGKVYALAGFTSG
                     VLLVLVSLWLFKEGIEKLVDYETVEYSEALPVAVIGLLVNLVSALLLSKGHTYGHHHH
                     GHSHDHDHDHAHAHDHDDHRDHAHAHKPPKVEAGTIDFNIRAAYIHILADALTSVLAI
                     IALSFGKYFGADAAGKPHFWYLDPLMGLVGGVVIVWWSISLCRQASRQLLDVVSSPRH
                     EEVVRRELEAIDDVRVADLHVWELGPGRRSCIVSVVTSTPRELDFYRDKVLTALPVAH
                     LTIEIHRCELPHDDVMRSNEVAHSHEH"
     gene            complement(3129..3617)
                     /locus_tag="VMZ53_32070"
     CDS             complement(3129..3617)
                     /locus_tag="VMZ53_32070"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUS33198.1"
                     /translation="MVTGLIKREHDEVAELFDHLSVLSRDDRRRDEASRTAARLVALV
                     RIHCRAEERVLYEALRTMPGPLKSFALAGPHEHEVLDTTLDKLLVRRPGDGEYQVIVR
                     VARDLFVMHARDEEEADILPLVEQELSPEELDSLAADLVAEQARIRPSVLRLVGMPAR
                     AA"
     gene            complement(3633..4064)
                     /locus_tag="VMZ53_32075"
     CDS             complement(3633..4064)
                     /locus_tag="VMZ53_32075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33199.1"
                     /translation="MPDNKNQNQNRGQGQKQQGTQKQDNDQGRQQQSNQGQEQGGQGR
                     QGRQQQSNQDQGQGGDRDSDRMSQGQNRGSQKGSTNTEQDTQNVSNQNDDMGNETEME
                     DQDEGQEATQSQGQGQERQEQGRNQASQDRGGQGRNGDRNR"
     gene            complement(4263..4979)
                     /locus_tag="VMZ53_32080"
     CDS             complement(4263..4979)
                     /locus_tag="VMZ53_32080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS33200.1"
                     /translation="MALLEIKDLDVHYGGIHALKGVSLSVEAGEIVTLIGANGAGKTT
                     TLRAISALVKPTRGSVKFAGADITHVPAHAIVSRGLVHAPEGRGIFSNMTVEENLEIG
                     AFLRKDSSGIAEDRSYALSLFPRLKERLKQNAGTLSGGEQQMLAIARAIMSRPKLLLL
                     DEPSLGLAPQIVALIFKIVKTIAARGTTILLVEQNAHMALGVANRAYVLEVGEIVLEG
                     PAKDLARNDEIRKAYLGIHD"
     gene            complement(4979..5809)
                     /locus_tag="VMZ53_32085"
     CDS             complement(4979..5809)
                     /locus_tag="VMZ53_32085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS33201.1"
                     /translation="MSALMLDIQKLSLSFGGLKAVSDFSMSLPVGCLYGLIGPNGAGK
                     TTVFNLLTGVYKPDAGHVYLDGAQVDGKKPSQIARAGMARTFQNIRLFGELSVIDNVR
                     TAAGVRAKTGLFRTLLRSPVHVGEERAITERSLELLDVLGITHRKDEQSKSLPYGDQR
                     RLEIARALATEPKVLLLDEPVAGMNTQEKREMRGLIQSLREKFGVSILLIEHDMGLVM
                     DICQQITVIDHGVTIAVGSPKEIQGNKAVIEAYLGVPDESDPIPAPNAPPETDSPEAI
                     "
     gene            complement(5806..6906)
                     /locus_tag="VMZ53_32090"
     CDS             complement(5806..6906)
                     /locus_tag="VMZ53_32090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUS33202.1"
                     /translation="MKPMQERVAGLRPHPISAIGSSLLPFVVAILLAFLIDKVVGPAV
                     GATWSKILLDVGIAILLAGSLNVVNGYAGQFSIGHAGFMLVGGYAAAWLTFYGSIHFF
                     GTKEATGGFLGNGQLLFLGGCLLAGLIACVFGVLVGLPSLRLRGDYLAIVTLGFGEIV
                     RVLGQMTARQPKRVVEIKERSLSELANNLGGAEGFSQIPSYTTLFFVYAFALILLVFA
                     YRLKYSSQGRALLAVRENEIAAEAMGVDTTRVKVVAFIFSAFFGGIGGSLYAHQVGAL
                     DPKELGFLKSIDLVIIVVIGGMGSITGVVIGAIILVVLPEVFRSFADYRMIVYALALI
                     IVMILRPQGIMGVHELWETSLWRKLADKVRKR"
     gene            complement(6915..7862)
                     /locus_tag="VMZ53_32095"
     CDS             complement(6915..7862)
                     /locus_tag="VMZ53_32095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUS33203.1"
                     /translation="MSDLLQTTINTLAVGGLYALIALGYTLVYGILRFINFAHSDVVA
                     WGAWIAFSLASYLGFMAAGSNPWISVPVVLLGTMALCAAMGVTIERLAYRPLRKAPRL
                     NVLITAIGVSLFLQNVGLQDKVFGNRPATIKALMPNKVLAVIDGVPLRLFDVLTIVLT
                     VVLVFALERIVYGTKMGRAMRAVSFDERVASLMGINVNIVVAVTFALGSALAAAGGIL
                     FALRNDLRMTADTSWVLLGLKAFVAAVVGGIGNVRGAAAGAMLIAFVEVFVHRYITGA
                     FPDKAWVGQLQDIYVFGILIVVLLFKPSGLLGKAVIEKV"
     gene            complement(7869..9056)
                     /locus_tag="VMZ53_32100"
     CDS             complement(7869..9056)
                     /locus_tag="VMZ53_32100"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS33204.1"
                     /translation="MNLNTWRGLAVALLSTLIALAGCDKGNKGGGGGSGGGGGGNASG
                     PIVIGHFASMSGPQATFGISTDQAIRLAIKEKNEKGGIKGRKIELVTIDDAGKQSEAA
                     TAVTRLINDHHAVAVLGEVASSLSLAGGPIAQKAHVPMISPSSTNPDVTDVGDYIFRV
                     CFLDDFQGWVDAKFAKENLKATKAAILYDQAQAYSSGLADYFEKSFKEMGGTIVTKQA
                     YTGGNLEISSQLQSVKSSGAEVVFLPGYYSDAGTIIRKAREAGITTPFVGGDGWDSEE
                     LPKIAGDAINGNYFSNHYAPEEDRPEVKNFVQKYQSEYHKTADGLAALGYDAALVLFD
                     AMERSKSLDGKDLRDAIAETKNFTGVTGTFSIDADRNAMKSAVIVEYKAGKQTMNARI
                     EKK"
     gene            9192..11270
                     /locus_tag="VMZ53_32105"
     CDS             9192..11270
                     /locus_tag="VMZ53_32105"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HUS33205.1"
                     /translation="MTNSSVAPHESGARRRRDQTGLHSAHGHGQHGQGHHQPHNRKEL
                     GLLSLGALGVVYGDIGTSPLYAMRECFISTNPHRADVEGGTLTFQNGVPIVTKFVQTD
                     NILGILSLFVWSLILVVIVKYLVFVLRADNKGEGGTLALAALVAQKQKGEVSRTRLRI
                     PILLALFGTGLLFGEGIITPAISVMSAVEGLKEQNHSLGALVVPISAGILIGLFWVQK
                     YGTGRIGSIFGWVMLVWFAAISIAGIPYIISYPGVLRALSPHYGAMFLATHGKQGFLL
                     LGSVVLCITGCEALYADMGHFGKSPIRIAWTWVVFPGLLLNYFGQGALYLHEGASVSS
                     PFYDLVSGTPFLIPMVILATMAAIIASQALISGAFSLANQAVQLGYLPRVKVVHTSSK
                     AEGQIYIPEINWILMVACLALVFFFQSSTKLASAYGIAVTGTMSITSFLFFRICRQNW
                     GWSLPAALGLYLPLIVIDYSFFSANLVKLGDGGWFPLAVGTAMFMIMTTWWRGRNALS
                     KMMETGTIPDQLFLQDIAATGIQRVSGTAVFMSSGTDGMPNVLLHHVKHNKVLHKQVV
                     LLSVITENVPFMTGNKSLSVIAIKHTDEEGKEHDMGFFRVMARVGFMQQPNVPKILAR
                     CEKHDLHVAPGDTTYYLGRQTLLTTGGSGLAKWRKLLFSFLARNSRPPTAFFNLPPNR
                     VVELGLQIEL"
     gene            11344..13248
                     /locus_tag="VMZ53_32110"
     CDS             11344..13248
                     /locus_tag="VMZ53_32110"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HUS33206.1"
                     /translation="MATAVHAVHTPATSKQRVSLALGALGVVYGDIGTSPLYAMNECL
                     LAKNKHAVTPDLANVLGVLSLFFWSLVLVVIVKYLVFVLRADNKGEGGILALAALVKK
                     GGRPNRRRLAIPILLALFGAGLLYGDGVITPAISVLGAMEGLSEQSPALTHFIVPISA
                     VILVALFAIQRHGTAKIGSIAGWIMLLWFVAIGLAGLRWIIREPIVLTAVSPHHAVLF
                     LAGHGMHGFLLLGLVVLCVTGTEALYADMGHFGRTPIRVAWSFVVFPGLLLNYFGQGA
                     LFLQHGTNVSNGFYDLVPAPLVIPMLVLATMAAITASQALISGAFSLTNQAVQLGYMP
                     RVKVVHTSPTMEGQIYIPEVNYLLMAACVTLVLVKQTSSSLASAYGIAVTGTMAITSF
                     LYFLVCRRNWNYSLGKALALLIPFLAIDLTFFSANAVKIAAGGWFPLAVGAVMFIVMT
                     TWWRGRNELSKMMEVGTLPDEQFLGDPMMSALPRVPGTAVFMSSGTDGMPNVLLHHVK
                     HNKVLHAQVVLLSVVTQSTPTVASDQSLEVRELGHGFYRVLANVGFIQQADVPKILAR
                     CAVHGLHVNAMDTTYYLGRQTLLTTGKAPVARWRKGVFAFLARNARTPTAFFNLPPNR
                     VVELGLQIEL"
     gene            13245..13436
                     /locus_tag="VMZ53_32115"
     CDS             13245..13436
                     /locus_tag="VMZ53_32115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33207.1"
                     /translation="MNEDHALCGGSIAIGASLVAAQLLDGGALGTGATIGICMLLLGA
                     RGLLCGYKLPSARVVRWRH"
     gene            13521..14597
                     /locus_tag="VMZ53_32120"
     CDS             13521..14597
                     /locus_tag="VMZ53_32120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HUS33208.1"
                     /translation="MADVRPHRAPPRAYVIAVVTVAIVTALGVALSPYTSHADQAMAF
                     MLAVLVAALGGRGPGLTAAVLSAAAFDFFFVEPVHTFAVADRSFLITFAVMLAVGAAI
                     GSLVSRVRAAEAESRERSLRARAEEMRATLLSSVSHDLRTPLAVITGTATSLRDASEG
                     AQKRQLETIVDEAQRLSRILTNLLSITKVESGTQPKREWVPLEELVGSALGRLKAELA
                     DHPLTLAISDEQAHVDPILCEQLLINLIENATKHTPDGTPIDVRVRRESRAAIIEVGD
                     RGPGLPEGRVEQVFEKWFRGSTTRVGGVGLGLAVCRAIVVAHDGVIEAVRRDGGGALF
                     RVTFPDDGAPPQLQDDEAELARAS"
     gene            14594..15289
                     /locus_tag="VMZ53_32125"
     CDS             14594..15289
                     /locus_tag="VMZ53_32125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS33209.1"
                     /translation="MTAADTVLVVEDEAPMRRFLRGALQAEGFRVIEASTLATAKLLV
                     TVHVPAAILLDLGLPDGDGLTLLESVRAWSQAPIIVVSARDREDDKVLALDAGADDYL
                     TKPFGTSELLARIRVALRHARAQRGPDDPVLVVGPIKIDQARHEVTNDGVQVHLTPTE
                     YRLLVMLARHAGKVITHRQLLTDVWGPRSTHQTHYLRVHMAALRRKIEADPARPRFLS
                     TEPGVGYRLLTDG"
     gene            complement(15291..15608)
                     /locus_tag="VMZ53_32130"
     CDS             complement(15291..15608)
                     /locus_tag="VMZ53_32130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33210.1"
                     /translation="MRIQALLLTAVFACGGPPNVGGTCTSADNCDDDLTCDTSVAGGY
                     CTASCTTSGSTDQCPEGSVCDSVSGAAVTCVKICKVAADCRSDLDCNGVSGTNIKACK
                     PKT"
     gene            complement(15618..16100)
                     /locus_tag="VMZ53_32135"
     CDS             complement(15618..16100)
                     /locus_tag="VMZ53_32135"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUS33211.1"
                     /translation="MKDDVLGYACSPVATLAAAAGTAALLVKHTPDEAARFAIAVPVA
                     CGIGKLLKKLVHEPRPELFLDDQPMESFPSGHSAAMTAFALSAVAATRRWWMLPIAAG
                     AVAAVNISRVRQREHWAWDVIAGDLVGVAGFAAGAAAAYAVRHARKRRRERAMNHAPG
                     "
     gene            complement(16097..16489)
                     /locus_tag="VMZ53_32140"
     CDS             complement(16097..16489)
                     /locus_tag="VMZ53_32140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33212.1"
                     /translation="MKKPELAPEQLRGTKLWEYAVRFVFGGAITVVTGLIANIWGPLV
                     AGLFLAFPAILPASMTLVKQHDGRRAALEDARGARLGSVGLAAFSATVAVCAGGLPAW
                     AVLLAAAAMWALVDIGLWFLRYSKEEAA"
     gene            complement(16486..16827)
                     /locus_tag="VMZ53_32145"
     CDS             complement(16486..16827)
                     /locus_tag="VMZ53_32145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33213.1"
                     /translation="MIRFLLGGVVVSAFAAAGELFKPKTFSGLFGAAPSVAIASLALA
                     FHAHGANYVRTEAKSMLIGSAAMLLYTIACIVVAKRDSIPVWLAAGLAWIAWFAAAFA
                     LFALARSGGLA"
     gene            complement(16904..17398)
                     /locus_tag="VMZ53_32150"
     CDS             complement(16904..17398)
                     /locus_tag="VMZ53_32150"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUS33214.1"
                     /translation="MTEFLTLLRRDHHDLEVGLDELMEAVSLSQLRSALDGVRLGLTA
                     HAEAEDIVLYKALQRIDDRAALESFVGQAREAHLAQEGALASLVCARPGTSTWRERAR
                     FLRNMVRDHAFFEEQEVVPRIRQVAPDVYDHLAGEFATERLRQLAQLQPSAPISIAEL
                     ARAS"
     gene            complement(17560..18855)
                     /locus_tag="VMZ53_32155"
     CDS             complement(17560..18855)
                     /locus_tag="VMZ53_32155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33215.1"
                     /translation="MSRVLTLAAVLAVAACGRLGFDFAGGGGSSTSPDATGVPDDTGV
                     LAAACPEKPMTSGIMVKTGTELVNAIASASPGDTILLADGLYSTASTISIDTPNLTIR
                     SASNHSDGPVVDGLGLANPLFYVRAKNVSFISLTMRNTKQDAVFVEPLDAAGDATGVR
                     IYDVTFTDVRGPAVRAKSYMSLATTPFADDGEVACSRVGHTVDSDCGPDGVFGVRLQG
                     ARNWTIRDTYFSGRCSPSRTRAVWADAGARDVTVTNNVFANNRNNILFGGIALRTYPD
                     ALPAGCSGTPHLWGGLVCNNRIGGLGVSSSTGNDFEEGIALWTTCDTWVLHNTVVSPA
                     GAETYENIEYRFAGTYVHLVNNLLSVDPLLRDSGQLDPASTNTLYTSPSDFVDAAAGD
                     LRISTSATIASGVAISQCLTDANGKPRNTASPTPGAFER"
     gene            complement(18873..>19129)
                     /locus_tag="VMZ53_32160"
     CDS             complement(18873..>19129)
                     /locus_tag="VMZ53_32160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33216.1"
                     /translation="ALPIVRAPTLLIAGGNDEHLIDLDRSVVRRMTTCTQLAIIPGAG
                     HAFDEAGMLDEVIALATEWLVDHFVIRPQFVAAAAVRAGA"
BASE COUNT         3297 a         6223 c         6168 g         3441 t
ORIGIN      
        1 cgatctctac gtcgatcccg atacgcaggg cacgctcgta gaagattgtg cggtctgctg
       61 ccggccatgg gccgtgagcg tctaccgcga cggcgatcgt cttcgcgtcc aagtctcccg
      121 cgcgcagtga cccgaaagac gatcgcgctc tgccgttcgc cgttcgccgt gcgccgtgcg
      181 ccgctcgccg ctcgccgctc gccgctcgcc gttcgccgtg cgccgttcgc cggaggcccg
      241 aaccggttgc gaacgtctgc tggtttcggc ggacccggat ggaatgcaag ggagcaagca
      301 gagcaaggga cgcaagtcgg aaggccggcg ggacgtcgcg cgatcgcccg ggagaacgca
      361 cgcgttccgg ccgtctgagc acacgtcctt gcggcgcgaa gcgcacgcat gcctccggct
      421 tgtgtccctc tccggccttg cccgttgcgt tccatccggg tctgccgaaa acgtggcggc
      481 gtcagcaacg ggttcgcgtt tcgcagcggc ggcgtgctgc ggcgcggcgt tagcggcggc
      541 ggcggcggcg gcgcagcacg aggccgagcg cgaggagcaa gcctgcaccg gtgcccgagc
      601 caccgcgtgc gttgcaaccg gacgactcgt cacccggcgg gtcgagctgg tcgtcacccg
      661 agcccgtgcc cggatcgacg cgcgtcacgc gcacgccgtc gaacaccatc tgcacgttgt
      721 ccgcgagcag ctcgcccgtg ttgtcgccga ggtgaatcgt ctgataggca cccgcctcga
      781 attggaactc gccgagcgac tgccagccat cgaccgccgt ctgatcgatc acgacgtcct
      841 tcgatgcggc gccggcttcg accgtgtact tcgccttctt cgattgagcg aacccaccgt
      901 cggtgtagac ctcgaccttg tagagcccgg cttcttcgaa ccagaggtgc catgtgccgt
      961 agttgccttc ggccgtgtct tccgtcgtgt gcgtccagac gaggtcaccg tcgaggccgg
     1021 ccgttgccac gtggcgcatc gaggctgcgg gaccgccgcc gaggaagcag gagtcgccgt
     1081 catcgatcac ggcgccggct ttgccgatcg tcgtgcaggg gccgcagtct tcggggcaat
     1141 cgagcgagcc ttcgccggac tcgcacgtgc tgttgccgca ggtggctgcg gggccgagga
     1201 acgcggtgag cgacgccatg tcgccattcc agcgattcat gtcgacgttg ccgctgatgc
     1261 cggcgaccgt gcccgtcgac gagtattgcc agagcgccca cttggaccac ggcggcgcga
     1321 tgttcgggca ggcggcgtcg gagtactgcg cgtgccacag cggcgacgac gtgacgtccg
     1381 gcgcgccgac agagtcacgc cagaaataga agccggtgta gatgatcgga tcgcgaccga
     1441 tcgcggcctt cacgtgggcg acccacttct tcaccttcgc ggtgatgacc gcggcggatt
     1501 ggccgtccga cgcttcgacg tcgatgacgg gaggcaggtc gtcagcttcg agcttgccgc
     1561 cgatcttctg gagcaggagg tccgcttgct cgatcggatc ctgaccgggg cggaagaact
     1621 gatacgcgcc gtgcttcacg cccgcggcgc gcgagcccga ccagttcgcc gcgaacttgg
     1681 agtcgatcgt gttgaggccg tcggagacgc ggatgaaggc gaacttcacg ccgtcgttct
     1741 tgaccgaggt ccaattgatg gtgccttggt agtacgagac gtcgatcccc ttcacgacgg
     1801 gaccgacgcc gcagaccgtt gcttcctgtt cggtgacgct ctccgtttcg gtgacgggat
     1861 ccgctgcaca accgacgaga attgcgagta ggcagagacg cttcacggtg caccaccgca
     1921 cagcaacatg gaggccaagc gccgatgcct ccatgtgacg agattgcgca cgcgtcgagg
     1981 ttgcagctgc aaacgatcga cgtcagtgct cgtggctgtg agcaacctcg tttgatcgca
     2041 tgacgtcgtc gtggggcaat tcgcaacggt ggatctcgat cgtcaggtgc gcgacgggca
     2101 gtgcggtcag gactttgtct cgatagaaat cgagctcgcg aggcgtcgag gtcacgacgg
     2161 agacgatgca gctgcgccga cctggcccca actcccacac atgtaggtcg gccaccctca
     2221 catcgtcgat cgcttcgagc tctcggcgca cgacctcttc gtgccgcggc gacgagacga
     2281 cgtcgagcaa ctgacgactc gcctgacgac agagactgat cgaccaccac acgatcacga
     2341 cgccaccgac gagacccatc agtggatcga ggtaccagaa gtgcggcttg cccgcggcgt
     2401 ctgcaccgaa gtacttgccg aacgacagcg cgatgatcgc gagcacggac gtcagcgcgt
     2461 ccgcgagaat gtggatgtac gcggcgcgga tgttgaagtc gatcgtgccg gcttcgacct
     2521 tcggtggctt gtgtgcgtgc gcgtgatcgc gatggtcgtc gtgatcgtgg gcgtgcgcgt
     2581 gatcgtgatc atgatcgtgc gagtggccgt gatggtgatg gccatacgtg tggcccttgc
     2641 tgagcagcag cgccgagacg agattgacga gcaggccgat aaccgcgacc ggcagcgctt
     2701 cgctgtattc gacagtttcg tagtcgacca gcttctcgat gccttctttg aagagccaca
     2761 gcgagacgag cacgaggagc acgccactcg tgaagcccgc gagcgcgtag accttgcccg
     2821 tgccgaagct gaatgcgtcg ttgccggcgt gacgacgcgc gtaccagtac gcgatgagcg
     2881 tgaagccaag cgcgccgacg tgcgtgccca tgtgccagcc atcggccttg agcgcagtcg
     2941 agttcgtgat gtgaccgacg atgagctcgc cgaccatcat caacgcggtg atgcccacga
     3001 cggcaagcgt gcggagctcg cccgatcgat tgtgctcgcc ctgctcttcg tgatgggcgt
     3061 gcgcggacag atcgtgggaa gtcgccatac ggcggcccca ctctaccagc tgcaaccctg
     3121 ttgcggaatt aggccgctcg ggcaggcatt ccgacaagac ggagaaccga cggccggatc
     3181 cgcgcctgtt cggcgaccag atcggcggcc aacgaatcga gctcctcggg cgacagctcc
     3241 tgctcgacga gcggcaggat gtccgcctct tcctcatcgc gagcgtgcat cacgaacagg
     3301 tcgcgcgcga cgcgaacgat gacttgatac tcgccgtcac cggggcgacg aacgagcagc
     3361 ttgtcgagcg tcgtgtcgag cacctcgtgc tcgtgcggtc ccgcgagcgc gaatgacttc
     3421 agcggccccg gcatggtccg caatgcttcg tagagcacgc gttcttcagc gcggcagtgg
     3481 atacgcacga gcgcgacgag gcgagccgct gttcggcttg cttcgtcgcg ccggcgatcg
     3541 tcgcgcgaaa gcacggagag gtggtcgaac agctcggcca cctcgtcgtg ttcgcgcttg
     3601 atgaggccag tgaccatcac caagtcacat tgctagcggt tgcgatcgcc gttgcgacct
     3661 tggccaccgc ggtcttggct cgcttggttg cggccctgct cttgacgctc ttggccttgc
     3721 ccctggctct gagtggcctc ctggccctcg tcctgatctt ccatctcggt ctcgttaccc
     3781 atgtcgtcgt tctggttgct gacgttctgg gtgtcctgct cggtgttcgt cgagcccttt
     3841 tgcgagccac ggttctggcc ctggctcata cgatcgctgt cgcgatctcc gccctggccc
     3901 tggtcttggt tgctctgctg ctggcgacct tgacgaccct gaccgccctg ctcctgaccc
     3961 tggttgctct gctgctggcg accctgatca ttgtcctgct tctgagtgcc ctgctgcttc
     4021 tgaccctgac cacgattctg gttctgattc ttgttgtccg gcatacgtcc cctccttttc
     4081 tgaagaagaa ggctgcaacg tatgtgcccc caagccgaca cgccccgttt acacatccga
     4141 agcaatcacc accgcgttga cgctacgacg agcgctcacg caccgcacga gatcgcgcgt
     4201 cgacgaatta ctccgtcgat ggcaattctg tcagccgaat ccgttcggag tatccagcag
     4261 cgctagtcgt gaataccgag atacgctttt cggatctcat cgttccgcgc aaggtccttc
     4321 gccggaccct cgagcacgat ctcgccgacc tcgaggacgt acgcgcgatt cgcgacacca
     4381 agtgccatgt gagcgttctg ctcgacgagc aggatcgtcg tgccgcgcgc agcgatggtc
     4441 ttcacgatct tgaagatcag cgcgacgatc tgcggcgcga gaccgagtga cggctcgtcg
     4501 agcagcagca gcttcggccg cgacatgatc gcccgcgcga tcgcgagcat ctgctgctcg
     4561 ccacccgaca gcgtgcccgc gttttgcttg agccgctctt tgagccgcgg gaacagcgac
     4621 agcgcgtagc tacggtcttc ggcgattccc gagctgtcct tgcgcaggaa tgcaccgatc
     4681 tcgaggttct cctcgacggt catgttcgag aagatgccgc ggccttccgg cgcatgaacg
     4741 aggccgcgcg agacgatcgc gtgggctggc acgtgggtga tgtcagcgcc cgcgaacttg
     4801 acgctgccgc gcgtcggctt cacgagcgcc gagatcgcgc gcagggtcgt cgtcttgccg
     4861 gcgccattcg cgccgatgag cgtcacgatc tcgcccgcct cgacgctgag cgacacgccc
     4921 ttgagcgcat ggatgccacc gtagtggacg tcgaggtctt tgatctcgag gagcgccact
     4981 agatggcctc cggagaatcg gtttccggtg gggcgttcgg cgccggaatc ggatcggatt
     5041 cgtccggcac gccgaggtac gcctcgatga cggctttgtt gccctggatc tctttgggcg
     5101 agcccaccgc gatcgtcacg ccgtgatcga tcaccgtgat ctgctggcag atgtccatca
     5161 cgagacccat atcgtgctcg atcagcagga tcgagacacc gaacttctcg cgcagcgatt
     5221 gaatcaggcc gcgcatctcg cgcttctcct gcgtgttcat gcccgcgacg ggctcgtcga
     5281 gcagcaggac cttcggctcc gtcgcgagcg cacgcgcgat ttcgaggcgc cgctgatcgc
     5341 cgtacggcag gctcttgctc tgctcgtcct tgcgatgggt gatgccgaga acatcgagga
     5401 gctcgaggct gcgctcggtg atcgcgcgct cttcaccgac gtgaacgggc gagcggagca
     5461 gcgtgcgaaa caggcccgtc ttcgcgcgca cgcccgcggc tgtacgaacg ttgtcgatca
     5521 ccgagagctc gccgaacagg cggatgttct ggaacgtacg cgccatgccc gcacgcgcga
     5581 tctgtgatgg tttcttgccg tcgacctgag cgccgtcgag atagacgtgg ccggcatcgg
     5641 gcttgtacac gccggtgagg agattgaaga ccgtcgtctt gcctgcgccg ttcgggccga
     5701 tgaggccgta gagacagcca accggcaggc tcatcgagaa gtcgctcacc gccttgaggc
     5761 cgccaaacga gagcgacagc ttctggatgt cgagcatcag cgcgctcatc gcttacgaac
     5821 cttgtcggcg agctttctcc agagcgaggt ctcccacagc tcgtgcacgc ccatgattcc
     5881 ctgcgggcgc aggatcatca cgatgatgag cgcgagcgcg tagacgatca tgcggtagtc
     5941 ggcgaatgac cggaacactt ccggcagaac gacgaggatg atcgcgccga tcacgacgcc
     6001 ggtgatcgac cccatgccgc cgatcacgac gatgatcacg agatcgatcg acttcaggaa
     6061 gccgagctcc ttgggatcga gcgcgccgac ttgatgcgcg tagagcgagc cgccgatgcc
     6121 gccgaagaac gcgctgaaga tgaacgcgac gaccttcacg cgtgtcgtat cgacgcccat
     6181 cgcctcggct gcgatttcgt tctcgcgcac ggcgagtagc gcccggccct ggctcgagta
     6241 cttcaggcgg tacgcgaaca cgagcaggat cagcgcgaac gcgtagacga agaacagcgt
     6301 cgtgtagctc ggaatctgcg agaagccttc ggcgccgccg aggttgttcg cgagctcgct
     6361 gagcgaccgc tccttgatct cgacgacgcg cttgggctgg cgtgcggtca tctggccgag
     6421 cacgcgcacg atctcgccga agccgagcgt gacgatcgcg aggtaatcgc cacgcaggcg
     6481 cagcgacggc aagccgacga gcacgccgaa cacgcacgcg atgagaccgg cgagcaagca
     6541 gccgccgagg aacagcagct ggccgttgcc gaggaatccg cccgtggctt ccttcgtgcc
     6601 gaagaagtga atgctgccgt agaacgtcag ccacgcggct gcgtagccac cgacgagcat
     6661 gaagcccgca tggccgatcg agaactggcc ggcgtagcca ttgacgacgt tgagcgagcc
     6721 ggcgagcaaa atcgcgatgc cgacgtcgag caggatcttc gaccacgtcg caccgaccgc
     6781 gggcccgacg accttgtcga tcaagaacgc gagcaggatc gcgacgacga atggcagcag
     6841 cgagctgccg atcgcactga tcgggtgcgg gcgaagaccc gcgacgcgct cctgcatcgg
     6901 cttcatcgac acgactagac cttctcgatg accgctttgc cgaggaggcc gcttggcttg
     6961 aacaggagga cgacgatcag gatgccgaac acgtagatgt cctgcagctg cccgacccac
     7021 gccttgtccg ggaaggcgcc ggtgatgtag cggtgcacga acacctcgac gaacgcgatg
     7081 agcatcgcgc ccgccgctgc accacgcacg ttgccgatgc cgccgacaac cgcagcgacg
     7141 aacgccttga ggccgagtag cacccacgac gtgtccgccg tcatgcggag gtcattgcgc
     7201 agcgcgaaca gaatgccgcc ggcggccgcg agcgcagagc cgagcgcgaa cgtcaccgcc
     7261 acgacgatgt tgacgttgat gcccatcagt gacgcgacgc gctcgtcgaa ggacaccgca
     7321 cgcatcgcgc ggcccatctt cgtgccgtag acgatgcgct cgagcgcgaa gacgaggacg
     7381 accgtgagca cgatcgtgag cacgtcgaac aggcgcagcg gcacaccgtc gatgacggcg
     7441 agcaccttgt tcggcatcag cgccttgatc gtcgccgggc ggttgccgaa caccttgtcc
     7501 tgcaggccga cgttctggag gaacagcgac acgccgatcg cggtgatcag cacgttgagg
     7561 cgcggcgcct tacgaagcgg ccgatacgcg aggcgctcga tcgtcacgcc catcgcggcg
     7621 cacagcgcca tcgtgccgag cagcacgacc ggaacggaaa tccacgggtt gctgcccgcg
     7681 gccatgaagc cgaggtacga agcgagcgaa aaggcgatcc acgcgcccca cgcgacgacg
     7741 tcagagtgcg cgaagttgat gaagcgaagg attccgtaga cgagcgtgta gccgagcgcg
     7801 atcagcgcgt agaggccgcc gaccgcgagc gtattgatcg tcgtctggag aagatcgctc
     7861 aacgcacgtc acttcttttc gatgcgagcg ttcatcgtct gcttgccggc cttgtactcg
     7921 acgatgacag cggacttcat cgcgttgcga tccgcgtcga tggagaacgt gccggtgacg
     7981 cccgtgaagt tcttcgtctc ggcaatcgcg tcacgcaggt ccttgccgtc gagcgacttg
     8041 ctgcgctcca tcgcgtcgaa caggacgagc gccgcgtcgt agccgagtgc cgcgaggccg
     8101 tcggccgtct tgtgatactc ggactggtac ttctgcacga agttcttcac ctcggggcga
     8161 tcttcctcgg gcgcgtagtg gttcgagaag tagttgccgt tgatcgcatc gcccgcgatc
     8221 ttcgggagct cttcgctgtc ccagccgtca ccgccgacga acggcgtggt gatgccggcc
     8281 tcgcgcgcct tgcggatgat cgtgccggcg tccgagtagt agcccgggag gaacacgact
     8341 tcggcgcccg acgacttcac gctctgcagc tgcgagctga tctcgaggtt gccgcccgtg
     8401 tacgcctgct tcgtgacgat cgtgccgccc atctctttga acgacttctc gaagtaatcg
     8461 gcgaggcccg aggagtacgc ctgcgcttgg tcgtagagga ttgccgcctt cgtcgccttg
     8521 aggttctcct tcgcgaactt cgcgtcgacc caaccttgga agtcatcgag aaagcagacg
     8581 cggaagatgt agtcgccgac gtcggtgacg tcaggattcg tcgacgaggg cgaaatcatc
     8641 ggcacgtgcg ccttctgcgc gatcgggccg cccgcgagcg agagcgacga cgccacctcg
     8701 ccgagcacgg cgaccgcgtg atggtcattg atcaagcgcg tgacggccgt tgcggcctcg
     8761 ctctgcttgc cggcgtcgtc gatcgtgacg agctcgatct tgcggccctt gatgccgccc
     8821 ttctcgttct tctctttgat cgcgagacga atcgcctgat cggtcgagat gccgaacgtc
     8881 gcctgcggac cggacatcga ggcgaaatgc ccgatgacga tcggccccga ggcattgccg
     8941 cctccgcccc cgccgctgcc accaccgccg cccttgttgc ccttgtcgca tcccgcgagc
     9001 gcgatcaacg tgctgagcaa cgcaaccgca agacctctcc aagtgttcag attcacggcg
     9061 atctccctta gataggcgcc ctcgataact gactgaaacc tcagtgtcaa ccgccaagac
     9121 cacggtgttg ttgatgagat cttgatccga tcgatggaaa tctttacggg ctgggtctac
     9181 caacgaggct cgtgacgaat tcttcggtcg cgccgcacga gtcgggggcc cgacggcgcc
     9241 gtgatcaaac cggtctgcac tccgcgcacg gccatggcca acatggtcaa gggcaccacc
     9301 agccgcataa ccgcaaggag ctggggctgc tctcgctcgg cgcgctcggc gtcgtttacg
     9361 gcgatatcgg tacctcgccg ctgtacgcga tgcgcgagtg cttcatcagc acgaacccgc
     9421 accgcgcaga cgtcgaaggc ggaacgctca cgttccagaa cggcgtgccg atcgtcacga
     9481 agttcgtcca gaccgacaac atcctcggca tcctctcgct gttcgtctgg tcgctgatcc
     9541 tcgtcgtcat cgtgaagtac ctcgtgttcg tgctccgcgc cgacaacaaa ggcgaaggcg
     9601 gcacgctcgc actcgcggca ctcgtcgcac agaaacaaaa gggcgaggtc tcgcgcacgc
     9661 gcctccggat cccgatcctg ctcgcgctgt tcggtacggg ccttctgttc ggcgaaggca
     9721 tcatcacgcc ggccatctcc gtgatgtccg cggtcgaagg cctcaaagag cagaaccaca
     9781 gcctcggtgc gctcgtcgtc ccgatctccg ccggcatcct cattggcctg ttctgggtgc
     9841 agaagtacgg aaccgggaga atcggctcga tcttcggctg ggtgatgctc gtgtggttcg
     9901 ccgcgatctc gatcgcaggc atcccctaca tcattagtta ccctggcgtg ctgcgcgcgc
     9961 tgtcgccgca ctacggcgcg atgttcctcg cgacgcacgg caagcaaggc ttcttgctgc
    10021 tcggctccgt cgtgctgtgc attaccggct gtgaagcgct ctacgccgac atgggccatt
    10081 tcgggaagtc gccgatccgc atcgcgtgga cgtgggtcgt gttcccgggt ctcttgctca
    10141 actacttcgg gcaaggcgcg ctctatctcc acgaaggcgc gagcgtgtcg agcccgttct
    10201 acgatctcgt cagcggcacg ccgttcctca tcccgatggt catcctcgcg acgatggccg
    10261 cgatcatcgc gtcgcaagcg ctcatctccg gcgcgttctc gctcgcaaac caagcggtgc
    10321 agctcggcta tctgccgcgc gtgaaggtcg tgcacacgtc gagcaaggcc gaaggccaga
    10381 tctacattcc agagatcaac tggatcttga tggtcgcgtg cctcgcgctc gtcttcttct
    10441 tccaatcgag cacgaagctc gcgtccgcgt acggcatcgc tgtgaccggc acgatgtcga
    10501 tcacgtcgtt cttgttcttc cggatctgcc ggcagaactg gggctggtcg ctccccgcgg
    10561 ccctcgggct ctacctgccg ctcatcgtca tcgactactc gttcttctcg gcgaacctcg
    10621 tcaagctcgg cgacggtggc tggttcccac tcgcagtcgg caccgcgatg ttcatgatca
    10681 tgacgacgtg gtggcgcggc cgcaacgcac tctcgaagat gatggagacc ggcacgattc
    10741 cggatcagct gttcctccaa gacatcgccg caacgggcat ccagcgcgtc agcggaacgg
    10801 ccgtgttcat gtcatcggga accgacggca tgcccaacgt gctgctgcat cacgtgaagc
    10861 acaacaaggt gctgcacaag caagtcgtgc tgctctccgt gatcaccgag aacgtgccgt
    10921 tcatgacggg caacaagtcg ctgtcggtga tcgcgatcaa gcacaccgat gaagaaggca
    10981 aagagcacga catgggcttc ttccgcgtga tggcgcgcgt cggcttcatg cagcagccga
    11041 acgtcccgaa gatcctcgcg cgctgcgaga agcacgatct gcacgttgcc ccgggcgaca
    11101 cgacgtacta cctcggccgc caaacgctgc tcacgacggg cgggtcaggg ctcgcgaagt
    11161 ggcgcaagct gctgttctcg ttcctcgcgc gtaactcgcg accgccgacg gcattcttca
    11221 acttgccgcc gaaccgcgtc gtcgagctcg gcctgcagat cgagctgtaa agaacgcatt
    11281 aagatttggg ctcgcgaaag ccatccgggc atcgtgccaa ctccacggtg gtagccctca
    11341 ggcgtggcca ccgcggtcca cgcagtacat acgcccgcta cttccaagca gcgggtcagt
    11401 ctcgcgctcg gagcactcgg tgtcgtctac ggcgacatcg gcacgtcccc gctctacgcg
    11461 atgaacgagt gcctgctcgc gaagaacaag cacgcggtga cgccggacct agcaaacgtg
    11521 ctcggcgtct tgtcgctgtt cttctggtcg ctcgtcctcg tcgtgatcgt gaagtacctc
    11581 gtgtttgtcc tgcgcgccga caacaagggc gaaggcggca tcctcgcgct cgcggcgctc
    11641 gtgaagaaag gcggtcgtcc gaatcggcgg cggctcgcga taccgatcct gctcgcgcta
    11701 ttcggcgccg gtttgctcta tggcgacgga gtaatcacgc cggcgatctc cgtgctcggc
    11761 gcgatggagg gcctgtccga gcagtcgccc gcgctgacgc acttcatcgt gccgatttct
    11821 gcggtgatcc tcgtcgcgct gttcgcgatc caacggcacg ggaccgcgaa gatcggctcg
    11881 atcgcaggtt ggatcatgct cctgtggttc gtcgcgatcg gcctggccgg tctgcgctgg
    11941 atcattcgcg agccgatcgt gctcacggcg gtgagcccgc accatgccgt gttgttcctc
    12001 gcgggacacg gcatgcacgg tttcttgctg ctcggtctcg tcgtgttgtg cgtgacgggc
    12061 accgaggctc tctatgccga catgggccac ttcggccgca cgccgattcg tgtcgcgtgg
    12121 tcgttcgtcg tgttcccggg gctgttgctc aactattttg gccaaggggc gctgttcctg
    12181 cagcacggca cgaacgtgtc gaatggcttc tacgatctcg tacccgcgcc gctcgtcatc
    12241 ccgatgctcg tgctggcgac gatggccgcg atcaccgcgt cgcaggcgct catctcgggc
    12301 gcgttctcgc tgaccaacca agcggtgcag ctcggctaca tgccgcgcgt caaggtcgtg
    12361 cacacgtctc cgacgatgga gggccaaatc tatatcccgg aggtcaacta cttgctcatg
    12421 gccgcatgtg tgacgctcgt gctcgtcaag cagacgagca gctcgctcgc gtcggcgtac
    12481 ggcatcgcgg tgaccggcac gatggcgatc acgtcgtttc tgtatttcct cgtgtgtcgc
    12541 cgcaactgga attactcgct cggcaaggcg ctcgcactgc tcatcccgtt tctcgcgatc
    12601 gatctgacgt tcttctcggc gaacgccgtg aagatcgcag ccggcggttg gtttccgctc
    12661 gcggtcggag ccgtgatgtt catcgtcatg accacgtggt ggcgcggccg caacgagctc
    12721 tcgaagatga tggaagtcgg gacgctgccc gacgagcaat tcctcggcga tccgatgatg
    12781 tcagcgctgc cacgtgttcc gggcacggcc gtgttcatgt catcgggcac cgacggcatg
    12841 ccgaatgtgc tgctgcatca cgtgaagcac aacaaggtgc tgcacgcgca ggtcgtgctg
    12901 ctctcggtcg tcacacagag cacgccgacc gtggcgagcg atcaatcgct cgaggtgcga
    12961 gagctcggcc acggcttcta tcgcgtgctc gcgaacgtcg gcttcatcca acaggcggac
    13021 gtgccgaaga tcctcgcgcg ctgcgccgtt cacgggctgc acgtcaacgc gatggacacc
    13081 acgtactacc tcggccgcca gacgctgctg acgacgggca aggcgccggt ggcgcgctgg
    13141 cgcaaaggcg tattcgcgtt ccttgcgcga aatgcgcgga cgccgaccgc gttcttcaac
    13201 ttgccgccga accgcgtcgt cgagctcggc ctgcaaatcg agctatgaac gaagaccacg
    13261 cactctgcgg cggttccatc gcgattggcg cgtcgctcgt ggccgcgcag ctcctcgacg
    13321 gcggcgcgct gggaacgggc gcgacgatcg gaatctgcat gctcctgctc ggggctcgcg
    13381 ggctgctgtg cggatacaag ctcccatcgg cgcgcgtggt ccgctggcgc cattagtgga
    13441 gaacgagcgc tacctgcgtg aacctcctcg gaaacttaga tcctctgtac gagaaatccg
    13501 aaagcggtac ggtgcggagc gtggcggacg tccggccgca tcgagcacct ccccgcgcgt
    13561 atgtcatcgc ggtggtcacc gtcgcgatcg tgacggccct tggtgtcgcg ttgtcgccgt
    13621 acacgagcca cgccgatcaa gcgatggctt tcatgctagc cgtgctcgtc gccgcgcttg
    13681 gtggccgcgg gccgggactg acagcggcgg tgttgtccgc cgcggcgttc gatttcttct
    13741 tcgtcgaacc ggtccacacc ttcgcagtcg cggaccgcag ctttctcatc acgttcgccg
    13801 tgatgctcgc agtcggagcc gcgatcggca gtctcgtcag tcgcgtgcga gccgccgagg
    13861 ccgagagtcg cgagcgttca ctgcgagcgc gtgccgagga gatgcgcgcg acgctgctct
    13921 ccagcgtctc ccacgacctg cgcacaccgc tcgccgttat caccggcacg gcgacgtcgt
    13981 tgcgcgatgc cagcgaaggc gctcagaaga gacagctcga gacaatcgtc gacgaggcac
    14041 agcgactgtc gcgcatcctg accaatctgc tctcgatcac gaaagtcgag tcgggcacgc
    14101 agccgaagcg cgagtgggtg ccgctcgagg agctcgtcgg gtcagcgctc ggccgtttga
    14161 aagcggagct cgccgaccac ccgctgacgc tggcgatcag cgatgagcag gcgcacgtcg
    14221 atccaattct gtgcgagcag ctgctcatca acctcatcga gaacgcgacg aagcacacgc
    14281 ccgacggcac gccgatcgat gtgcgcgtaa ggcgtgagtc gcgcgcggcg atcatcgagg
    14341 ttggcgatcg cgggccggga ttgccggagg gccgggtcga gcaggtcttc gagaaatggt
    14401 ttcggggcag cacgacgcgc gtcggcgggg tgggccttgg cctcgcggtg tgtcgtgcga
    14461 tcgtcgtcgc acatgatggc gtgatcgagg cggttcgccg cgatggtggc ggtgcgctgt
    14521 ttcgcgtcac gttcccggac gatggtgcgc cgcctcagct gcaggacgac gaagcagagc
    14581 tggcacgggc atcatgaccg ccgccgacac cgtgctcgtg gtcgaggatg aagcgccgat
    14641 gcggcggttc ctgcgcggag cgctgcaagc ggagggcttt cgcgtcatcg aagcgagcac
    14701 gctcgcgacc gcaaagttgc tcgtcaccgt gcatgtacct gcggcgatct tgctcgacct
    14761 cgggcttccc gacggcgacg ggctcacgct gctcgagagc gtgcgcgcgt ggtcccaagc
    14821 accgatcatt gtcgtgtcag cgcgcgaccg cgaagacgac aaggtgctcg cgctcgacgc
    14881 aggcgcggac gactacctga cgaaaccatt cggcacgagt gagctgctcg cacgaattcg
    14941 cgtcgcgctc cgccacgcgc gcgcgcagcg cggccccgac gatcccgtgc tcgtcgtcgg
    15001 tccgatcaag atcgatcaag ctcgtcacga ggtcacgaac gacggcgtgc aagtccacct
    15061 cacgccgacg gagtaccggc tcctcgtcat gctcgcgcgc cacgccggca aggtgatcac
    15121 gcatcgccag ttgctcacgg acgtgtgggg cccgcgaagc acgcatcaaa cgcactacct
    15181 gcgcgtccac atggcggcgc tgcgccgcaa gatcgaagcg gatccggcgc ggccacgctt
    15241 cctctcgacg gagcccggcg tcggctatcg cctgctcacc gacggttagc tcacgtcttg
    15301 ggcttgcacg ccttgatgtt cgttccgctc acgccgttgc agtcgagatc cgagcggcag
    15361 tctgcggcga ccttgcagat cttcacgcac gtcacggcgg cgcccgacac cgagtcgcag
    15421 accgagccct cagggcattg atcggtagac cctgacgtcg tgcagctcgc cgtgcagtag
    15481 ccgcccgcaa cgctcgtgtc gcacgtcaga tcgtcgtcgc agttgtcggc cgacgtgcag
    15541 gtgcctccga cattcggcgg acctccgcac gcgaagaccg ccgtgagcag cagggcttgg
    15601 atacgcatgc gtcgatgtta acccggcgcg tggttcatag cgcgttcgcg ccttcgcttg
    15661 cgtgcatggc gtacggcata cgcagccgcc gcacccgctg cgaaccccgc gaccccgacg
    15721 aggtcaccgg cgatcacgtc ccatgcccag tgctcgcgct ggcgtacgcg cgagatgttc
    15781 acggcagcga ccgcgccggc tgcgatcgga agcatccacc agcggcgcgt agcggcaacc
    15841 gcggataggg cgaacgcggt catcgcggcg gagtgaccac tcggaaacga ctccatcggt
    15901 tgatcgtcga ggaagagctc gggtcgaggc tcgtggacca acttcttgag cagctttccg
    15961 atgccgcatg cgaccggcac tgcgatcgca aaccgggcgg cctcgtcggg agtgtgcttg
    16021 acgagaagtg ccgcagtgcc agccgcagcg gcgagcgttg cgaccggcga gcacgcgtat
    16081 ccgagcacgt catctttcat gcagcttctt ccttcgagta gcgcaggaac cacagcccga
    16141 tatccacgag tgcccacatg gcagcagccg caaggagcac ggcccatgcc ggaaggccgc
    16201 ctgcgcacac ggcgaccgtt gcgctgaacg cagcgaggcc gacgctgccg aggcgagcac
    16261 cacgcgcgtc ttcgagcgcg gcacgccggc cgtcgtgttg cttcacgagc gtcatgctcg
    16321 cggggaggat ggcgggaaat gccaggaaga ggccggccac gagggggccc cagatgttgg
    16381 cgatgaggcc ggtcacgacg gtgatcgcac cgccgaacac gaagcgcacc gcgtactccc
    16441 agagcttggt gccgcgcagc tgttcgggcg cgagctcggg cttcttcatg cgaggcctcc
    16501 gctgcgggcg agcgcgaaca gcgcgaacgc ggccgcaaac catgcgatcc acgcgagccc
    16561 cgctgcgagc caaaccggga tcgaatcgcg cttcgccacg acgatgcaag cgatcgtgta
    16621 gagcagcatc gcggcgctgc cgatcagcat cgacttcgcc tcggtgcgaa cgtagttggc
    16681 gccgtgcgcg tggaacgcaa gcgcgagcga agcgatcgct accgatggcg ccgctccgaa
    16741 caggcccgag aacgtcttgg gcttgaacag ctcgccagct gcggcgaacg ccgagacgac
    16801 gacgccgccg agcagaaatc gaatcacgag ctccacacgc tagaaacgag caggcgacgt
    16861 gccagtcacc ggcgtgcgaa tgcgcgcggg ctcgcgcgta acctcagcta gcgcgtgcga
    16921 gctcggcgat cgagatcggc gctgagggct gcagctgtgc gagctggcgc aagcgctcgg
    16981 ttgcgaactc gcccgcgaga tgatcgtaaa cgtcaggcgc gacttgccgg atgcgaggca
    17041 cgacttcttg ctcctcgaag aaggcgtggt cacggaccat gttgcggagg aagcgcgcac
    17101 gctcacgcca cgtcgacgtt ccggggcgag cgcacacgag agatgcgaga gctccttcct
    17161 gcgcgagatg ggcctcgcgt gcctgaccga cgaatgattc cagcgcagct cgatcatcga
    17221 tccgttgcag cgccttgtag aggacgatgt cctcggcttc ggcgtgggcg gtcaatccga
    17281 gccgtactcc gtcgagcgcg ctgcgcaact gagagagcga taccgcctcc atcagctcgt
    17341 ctagtccgac ttcgagatcg tgatggtctc ggcgcagcag cgtgaggaac tcggtcatgg
    17401 gtgtcggatc gtgcagtgtg aatgccgcca cagccggacg ttgtgaccgg cacacccagc
    17461 gcgtgagtgc gaggtcgcat acgacgatcc gacgagaaat aacggttttg ctgagcaatt
    17521 cctcgacgtg acggcgcgcc tgtgtgcgcc gtggtcgcgc taccgctcga atgcccccgg
    17581 cgtgggcgac gctgtgtttc gcggcttgcc atttgcgtcg gttaggcatt gggagatcgc
    17641 tacgccgctc gcgatcgttg cactcgtcga gatccgtagg tcgcccgcgg ctgcatcgac
    17701 gaagtcgctc ggcgatgtgt agagcgtatt tgtcgacgcg ggatcgagct ggccactgtc
    17761 gcgaagcaac ggatcgaccg acagcaggtt gttcacgagg tggacgtatg ttccagcgaa
    17821 ccggtactcg atgttctcgt acgtctccgc gcccgcgggg gacacgaccg tgttgtgcag
    17881 cacccacgtg tcgcacgtcg tccagagcgc gatgccttct tcgaagtcat tacctgtcga
    17941 cgacgagaca ccgagaccgc cgatgcggtt gttgcagacg agcccgcccc acaaatgagg
    18001 cgtgcccgag cagcccgccg gtaacgcatc gggatacgtg cgaagcgcga taccgccgaa
    18061 caggatgttg ttccgattgt tcgcgaacac gttgttcgtg accgtcacgt cgcgtgcgcc
    18121 ggcgtcggcc cacaccgcgc gcgtcctcga aggagagcag cgaccggaga agtacgtgtc
    18181 gcgaatcgtc cagttgcgag cgccttggag tcgaacgccg aacacgccgt cggggccgca
    18241 gtcggaatcg acggtgtggc cgacgcgaga gcacgccact tcaccgtcat cggcgaatgg
    18301 agtcgtcgcg agagacatgt atgacttcgc gcgtaccgcg gggccgcgaa cgtctgtgaa
    18361 cgtcacgtcg taaatgcgaa cacctgtcgc atcgcccgcg gcatcgagcg gctcgacgaa
    18421 gacagcgtct tgtttcgtgt tgcgcatcgt cagcgagatg aacgagacgt tcttcgcacg
    18481 gacgtagaac agcgggttcg cgaggccgag accatcgacg actggaccat ccgagtgatt
    18541 gctcgccgat cgaatcgtga ggttcggcgt gtcgatcgag atcgtcgatg ccgtgctgta
    18601 caacccgtcc gcgagcagga tcgtgtcacc cggcgaggca ctcgcgatcg cgttgacgag
    18661 ctctgtgccg gtcttgacca tgatcccgga cgtcatcggc ttctcgggac atgcggccgc
    18721 gagcacgccc gtatcgtcgg gaacgccggt cgcgtcgggc gaggtgctcg agccgccgcc
    18781 gcctgcgaag tcaaaaccga gccggccaca cgctgcgacc gccaggacgg cggctagcgt
    18841 caggactcgg ctcatgctgc gatcgtactg cgttacgcac ctgcgcgaac ggccgctgcg
    18901 gcgacgaatt gcggccggat cacgaagtga tcgacgagcc actcggtcgc gagcgcgatg
    18961 acttcgtcga gcatgccggc ctcgtcgaac gcgtgtccgg cgcccgggat gatcgcgagc
    19021 tgtgtgcacg tcgtcatgcg tcgaacgacg ctgcgatcga gatcgatcag atgctcgtca
    19081 ttgccgcccg cgatcaggag cgtcggtgcg cgcacgatgg gcagcgcgt
//
LOCUS       DATLBZ010000654         3544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7363375, whole genome shotgun sequence.
ACCESSION   DATLBZ010000654 DATLBZ010000000
VERSION     DATLBZ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7363375"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VMZ53_32165"
     CDS             <1..529
                     /locus_tag="VMZ53_32165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HUS33217.1"
                     /translation="LCNQSDVPLLFLQNITGFMVGKKVEQEGIIKHGAKLINAVSNST
                     VPAITIMTGASYGAGNYAMSGRAYAPRFLYTWPNHRIAVMGGKQLAGVLDIIQRDAAA
                     KRGESVDEQKLGVMKQMTESMIEKESDPYFATARMWDDGIIDPRDTRTVIAISLSAAY
                     SAPFKGATSWGVFRH"
     gene            complement(533..1627)
                     /locus_tag="VMZ53_32170"
     CDS             complement(533..1627)
                     /locus_tag="VMZ53_32170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33218.1"
                     /translation="MDRVAAFIRANEARIARQWEDEVRGELPALRSMSRPVLLDHMQE
                     FLEGLAHWIEGDSENAERAFNCLVEGHALQRLGYGVGLETLTREYSKIRLVVLRMLGK
                     EKDLDPESMLRLHEGMDRAMHDAIRSYAMKREEVRERFMAILGHDLRDPLSTVKISAN
                     ILAANPSLKPELRLVASRISRATGRMHRMINDVLDFARGHLGNGIPANPTLSDMAEIC
                     TAAVDEIAAANPQRHIAVDTQGDLRGAFDRDRVHQALGNLIGNALHHTTGAIEVRAYE
                     EENHEAVVTEVTSHGAAIPENMRRRIFDPFAQGDTERKQGLGLGLYIVQQIALAHGGT
                     CDVASDETATTFSIRWPRAPGAERRKTAAG"
     gene            complement(1725..2189)
                     /locus_tag="VMZ53_32175"
     CDS             complement(1725..2189)
                     /locus_tag="VMZ53_32175"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HUS33219.1"
                     /translation="MGQTLGTGDKKSVNVELNIVPFIDLMSCLTAFLLVTAVWVNIAN
                     VKNEPVNQGPDPKPQGEPPPRLTVLLEDDQILVQQMPTGEARKLPAFDWTSLESTLRE
                     FKTPGETPTVEVAAASTNAHPIEYQKLIVAMDTAVRAGYPHVGVTDPMTLAR"
     gene            complement(2224..>3544)
                     /locus_tag="VMZ53_32180"
     CDS             complement(2224..>3544)
                     /locus_tag="VMZ53_32180"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HUS33220.1"
                     /translation="MMHNADPDNSFDEIAYQKAQVVLTTFEDFLGADAFQKVLRAYVG
                     THRGGTATTADFVSALRDATSAETATSFDQYVRSTGVPIVEMKRDCTAKQLALHARDR
                     RIVPVCVDINTKQPVCRLVGDDAVVELPASCPETFGNSRAGYYHTVWLSDGPHGSTKK
                     LDRKAERLRIVAGDDIAARIERGELTGVEALGELREFLDSGDSHTVLGGIAIARALDR
                     LVGEAERPAWTAWLAKHVQGMRADNMSELAEQLQIARLELAPTSYSPSVVKDTAMAVD
                     RQLAAMSITPMLVAIVAPSGGDKLFARIADRAKTAPADEREEFYSSLGVFGAAQAPLA
                     VALFADASIDAGDAWLAVEPMLSRPATRNATWRALKPVLPAVMKRMADDADELVATLG
                     SLCDQVSRDEVAAIDRGKTRQSASLAAIDRCIASRAKLGDLAGALAH"
BASE COUNT          587 a         1151 c         1149 g          657 t
ORIGIN      
        1 gctgtgcaac cagtcggacg tgccgctact gttcttgcag aacatcaccg ggttcatggt
       61 cggcaagaag gtcgagcagg aaggcatcat caagcacggc gcgaagctca tcaacgcggt
      121 gtcgaactcg acggtgccgg ccatcacgat catgaccggc gcgagctacg gcgctggcaa
      181 ctacgcgatg agcgggcgcg cgtatgcccc gcgcttcttg tacacgtggc caaatcatcg
      241 gatcgcggtg atgggcggta agcagctcgc cggcgtcctc gacatcatcc agcgcgatgc
      301 ggcggccaag cgcggcgagt ccgtcgacga gcagaagctc ggcgtcatga agcagatgac
      361 ggagtcgatg atcgagaagg aaagcgatcc gtacttcgcg accgctcgca tgtgggacga
      421 cggcatcatc gatccgcgcg acacgcgcac cgtcatcgcg atctcgctgt ccgccgcgta
      481 ctcggctcca ttcaaaggag cgacgagctg gggcgtcttc cgccactagt cgctagccgg
      541 cggcggtctt gcggcgctct gcaccgggcg cacgcggcca gcggatcgag aacgtcgtcg
      601 cagtctcgtc ggacgcgacg tcgcacgtcc cgccatgcgc gagcgcgatc tgctggacga
      661 tgtacagacc gaggccgagg ccctgcttcc tttcggtatc gccctgtgcg aacggatcga
      721 aaatccgacg ccgcatgttc tcgggaatcg ccgcgccgtg gctcgtgacc tcggtgacca
      781 cggcttcgtg attttcttct tcgtacgcgc ggacctcgat ggcgccggtc gtgtgatgaa
      841 gcgcgttgcc gatcaggttg ccgagcgctt ggtgcacgcg atcgcgatcg aacgcgccgc
      901 gcagatcgcc ttgcgtgtcg accgcgatgt ggcgctgcgg gttcgcagcc gcgatttcgt
      961 cgacggcggc ggtgcagatt tcggccatgt cgctcagcgt gggattcgcc gggatgccgt
     1021 tgccgaggtg accgcgtgcg aagtcgagca cgtcattgat catgcgatgc atgcgtccgg
     1081 tcgcgcggct gatgcgcgag gcgacgagcc gcagctcggg cttcagcgac gggttcgcgg
     1141 cgagaatgtt cgccgagatc tttacggtgc tgagcgggtc gcgcaggtcg tgaccgagga
     1201 tcgccatgaa gcgctcgcgg acttcttcgc gtttcatcgc gtagctgcga atcgcgtcgt
     1261 gcatcgctct gtccatgcct tcatgaaggc ggagcatcga ctcgggatcg agatcctttt
     1321 ctttgccgag catgcgcagg acgacgagac ggatcttcga atactcgcgc gtgagcgttt
     1381 cgagaccgac gccgtagccg aggcgctgca acgcgtggcc ttcaacgagg cagttgaaag
     1441 cgcgctctgc gttttcgcta tcgccttcga tccaatgcgc gagtccctcg aggaactcct
     1501 gcatgtgatc gaggagcact ggacgcgaca tcgaccgcaa cgccggcaac tctccccgca
     1561 cctcgtcttc ccactgcctt gcgatgcgtg cttcgttcgc gcgaatgaag gcggcaacgc
     1621 gatccatgaa tccaccgtag cggacgtttc ccgacatcgc atcccttaag agatcaccta
     1681 tttttccgcg cgcgctcgcg tcacagcgcg cgtgaatgcg cgcgtcagcg ggcgagcgtc
     1741 atcgggtcag tcacgccgac gtgggggtat ccggcgcgca cagctgtatc catcgctacg
     1801 atcaatttct ggtactcgat gggatgcgcg ttcgtgctgg ccgcggcgac ctccaccgtg
     1861 ggcgtctcgc ccggtgtctt gaattcgcgc agtgtggact cgaggctcgt ccaatcaaac
     1921 gccggtagct tgcgtgcctc gccggtcggc atctgctgca cgaggatctg atcgtcttcg
     1981 agcaagacgg tgaggcgcgg cggcggctcg ccttgtggtt tcggatccgg cccttgattc
     2041 acgggctcgt tcttcacgtt cgcaatgttc acccacaccg cagtcacgag caggaacgcg
     2101 gtgaggcagc tcatcaggtc gatgaacggc acgatgttga gctcgacgtt gacggatttc
     2161 ttgtcaccag taccaagcgt ttgacccatg cccaaaagac acgatcgctt gggggctgtt
     2221 gcatcagtgg gcgagggcgc cggccagatc gccgagcttt gcccgacttg cgatgcaacg
     2281 gtcgatagcg gcaagggaag ctgactggcg cgttttgcca cggtcgatcg cggcgacttc
     2341 gtcgcggctc acttggtcgc acagcgagcc gagcgtggcg acaagctcat cggcgtcatc
     2401 cgccatgcgc ttcatcaccg ccggcagcac cggcttcagt gcgcgccacg tcgcgttgcg
     2461 cgtcgcaggt cgcgacagca tcggctcgac agcaagccat gcgtcgccgg catcgatcga
     2521 ggcatccgca aacagcgcga ccgcgagcgg tgcttgcgcc gcgccgaaca caccgagcga
     2581 cgaatagaat tcctcgcgtt cgtcggcggg cgccgtcttc gcgcgatcgg cgatgcgcgc
     2641 gaacagcttg tcgccgccac tcggcgcgac gatcgccacg agcatcggcg tgatcgacat
     2701 cgccgcgagc tggcgatcga ctgccatcgc ggtgtccttc acgacgctcg gcgaatacga
     2761 cgttggcgcg agctcgagac gcgcgatctg cagctgctcc gcgagctccg acatgttgtc
     2821 ggcgcgcata ccctgcacgt gcttcgcgag ccacgccgtc caggcgggtc gctcggcctc
     2881 accgacgagc cggtcgagcg cgcgcgcgat cgcgatgccg ccgagcaccg tgtgtgaatc
     2941 gcccgagtcg aggaattcac gcagctcgcc gagcgcctcg acaccggtca gctcgccgcg
     3001 ctcgatgcgt gctgcgatgt catcccccgc gacgatgcgc agccgctccg ccttccgatc
     3061 gagcttcttc gtcgatccgt gcgggccgtc cgacagccac accgtgtggt agtagcccgc
     3121 gcggctgttg ccgaacgtct ccgggcagct cgccggaagc tcgaccacgg cgtcgtctcc
     3181 gacgagcctg cacaccggct gcttagtgtt gatatcgaca cagaccggaa cgatgcggcg
     3241 atcgcgcgca tgcagcgcga gctgcttcgc cgtgcagtcg cgcttcatct cgacgatcgg
     3301 tacgccagtc gagcgcacgt actgatcgaa cgacgtcgct gtctcggcac tcgtcgcgtc
     3361 gcggagcgca gacacgaagt ccgcggtcgt tgcggtgccg ccccggtgcg tgccgacgta
     3421 cgcgcgcagc actttctgga atgcgtccgc gccgaggaag tcctcgaacg tcgtgagcac
     3481 gacctgcgcc ttctggtacg cgatctcgtc gaacgagttg tcgggatcgg cgttgtgcat
     3541 catc
//
LOCUS       DATLBZ010000655         6440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8856515, whole genome shotgun sequence.
ACCESSION   DATLBZ010000655 DATLBZ010000000
VERSION     DATLBZ010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8856515"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VMZ53_32185"
     CDS             complement(<1..1022)
                     /locus_tag="VMZ53_32185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33221.1"
                     /translation="MREVAAKVDFGEIGTAVGDNALEHECGRGGRVEKGSQLYRFTYL
                     EKGGYGRWEIELREQQIRDIAAGLIEEIEAVQLAEGTRANRGDALIVWGEYDEDGLRV
                     RTLSDLAIVLDGMAASSMIAPCLVRLWGTSDQQAIAALNGLDAAVYVIESEQGYGRSV
                     GDPTRTETFRIIDHDVGSIDIPWADVVPWRIVRDALLRFVEYGDLGDQVILDGSIPTQ
                     FLMLGDYDRQAELASRRPPPMDPAQSSLPQKAPHGEWAKRLLRTLIELQLIEIDMHIV
                     DAILARLAILLVAHGDDALDAPEPAQQLAKQISKVRGVGALFATGGDLQIALRRTQEP
                     PTMPVE"
     gene            complement(1108..3105)
                     /locus_tag="VMZ53_32190"
     CDS             complement(1108..3105)
                     /locus_tag="VMZ53_32190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33222.1"
                     /translation="MRIAPYLVIALATIGCKKREEPQPRATGSGSAGMTQPSKSTHDA
                     VDRMVFNRTAVHLNLPIYWSADANNNKVLDPNEVAPLLFYPTQGNYVKDGAFTKDFED
                     AYAKIAEASKAAPPSDKRRQLVIEDLDAGRPSLVFTDLTKASAEDKAFVEKMLVVASL
                     LDNLYDTTNGNIAITKEVPPDPESQSLFRRNRGPKCVGPTTTANPECNVIPGLKKQIF
                     DIYPAEIQAKDTFCADLQARKDGNALMGHFNVVRGSGDALEAVPYNVAYKEQTAKIAE
                     ALRDAAKAVTDPNEKALVTYLNAAADGFTTNDWNPADEAWAKMSVDNSRWYVRAAPDE
                     TYWEPCAQKAGFHLTFAKINQGSKDWQGKLAPVLQEMEDAIAARAGAPYKSRKVAVHL
                     PDFIDIVVNAADDRDPLGATIGQSLPNWGPVANEGRGRTVAMVNINSDPDSRAARKQQ
                     AMSMLDAATVKLYPESTEPGLLSTILHEATHNLGPAHEYKVKGKVDDDVFGGPIAAMM
                     EELKAQTGAYFLLDFLVKKNIITQEFAQQTVADMLVWSFGHISQGMYTGEHNRKAYGN
                     LAAIQIGFMIDKGVLTWDANATAANGTDKGAFTLHPDKVTAVAEEMMKEFAGIKARGD
                     KAAAEKLIARYVDSDAVVPHKVISERVLRHPKPSFVYSVAF"
     gene            complement(3116..3796)
                     /locus_tag="VMZ53_32195"
     CDS             complement(3116..3796)
                     /locus_tag="VMZ53_32195"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUS33223.1"
                     /translation="MRAAPKVKKAKRSTTVPKRATGVQLGSDVARNMIMFGATRVFAT
                     KGFRNVSVEDLLEVSQVSRRTFYRFFKSKEDVALAMYTMGTSSLLEACRRAITSESDL
                     LKQLEKCIDLHLSNTRQMGRLIWVLGGEAQSLESPLHARRMEVHDQLISMFREASSSH
                     GNVDPLLIQTLIFALEQVVRAVLEQGDEGRRVTTESIARARAVMMRVATGAVAGTGPR
                     VAPLPLLS"
     gene            complement(3840..5123)
                     /locus_tag="VMZ53_32200"
     CDS             complement(3840..5123)
                     /locus_tag="VMZ53_32200"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HUS33224.1"
                     /translation="MSRRLSLLLFVLGCSGNAPDAGDPDAAADSSSSTVDAHFAPTLA
                     STQQLCKLLSDRNVNDPSANDVQHNSNVLGADLGIPVAANDKLYILFGDTIGFAGIWP
                     INESHPDSVGYGLDSATAIAANPALLCERMRIVSLPAAQSIGPSVNAAVKADFAGAAM
                     IAPSGHGLGEYIRNPAGADGNTFAQLPGDFEVPSGAFSVNGAIYVFYTTVTGRANPDM
                     KASYLAKWAQPATTGAFAYQILYQVDERFDANGPLHGHFVNVAAEVVGDYLYIFGTGD
                     YRASKIYAARKPLAQLEQPGGFEELGEIVGTAGYGEISVHYYPSIERWMLLAEELTPT
                     SNRIIARFATQPDAWDAPIIVHDMGDTTFTAKYCCSSEDNCLGKQMFNCNRTGFYGTY
                     LFPTLNETATELTVTYTMSSFSPYNVAVFQATFTK"
     gene            complement(5126..6130)
                     /locus_tag="VMZ53_32205"
     CDS             complement(5126..6130)
                     /locus_tag="VMZ53_32205"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HUS33225.1"
                     /translation="MYRFTFLFLTACVADVSGTEVPDTDPITEEGTRSGSGSASELTL
                     EAQQPWKVLEHQYQAQQTGYWCGPASTRMALSTISANLPTQQMLANELPTSTNGTDHI
                     GLVVNALNQHVGARYAAVLMPHDPPTAQERDQLWRDIRLSIDAGHGIVANIVAPASNH
                     PPGYPGYTIYHYISLIGYNADTKQVYVADPARFSGIEHYWLSFDQAATLIPPKGYATY
                     RCGVGRTIGDINAHYQALGGCGSFLGPALTDELTTPDGAGRYNVFANGSIYWSPFTGA
                     FEVHGAIRDKYMETGWEAGPLGYPVSDEHDVAGGRRSDFEHGAITWDAATHIATVTMN
                     "
     gene            complement(6140..>6440)
                     /locus_tag="VMZ53_32210"
     CDS             complement(6140..>6440)
                     /locus_tag="VMZ53_32210"
                     /inference="COORDINATES: protein motif:HMM:NF019913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33226.1"
                     /translation="ALGGCASIVGAPITAEAGTPDGIGRYSVFERGSIYWTPQIGAYE
                     VHGRIRDKWKDEGWEAGPLGYPTSDEYAVPGGRRSDFEHGSITWIAQTDTATATY"
BASE COUNT         1062 a         2084 c         2064 g         1230 t
ORIGIN      
        1 atctcgacgg gcatcgtcgg cggctcttgc gtgcggcgca gcgcgatctg cagatcgcca
       61 cctgtcgcga acagcgcgcc gacgcctcgc accttcgaga tctgctttgc cagctgttga
      121 gcgggctcgg gcgcgtcgag cgcgtcgtcg ccgtgcgcga cgagcaagat cgcaagccgc
      181 gcgaggatcg catcgacgat gtgcatgtcg atctcgatca gctgcagctc gatcagcgtt
      241 cgcaacaggc gttttgccca ctctccgtgc ggcgccttct gcggcaagct cgattgcgcg
      301 ggatccatcg gcggcggccg ccgcgatgca agctctgctt gccggtcgta gtcaccgagc
      361 atcaagaact gcgtcggaat cgaaccgtcg aggatcacct gatcaccgag gtcgccgtac
      421 tcgacaaagc gcagtaaggc gtcgcgcacg atgcgccagg ggacgacgtc ggcccacggg
      481 atgtcgatcg aaccaacgtc gtgatcgatg atgcgaaacg tctcggtgcg cgtcggatcg
      541 ccgacggaac ggccatagcc ttgctccgat tcgatcacgt agaccgcggc atcgaggccg
      601 ttgagcgctg cgatcgcctg ttgatcgctc gttccccaca gcctgacgag gcatggcgcg
      661 atcatgctcg acgccgccat cccatcgagg acgatcgcga gatccgacaa ggtgcgcacg
      721 cgcaagccgt cttcgtcgta ctcgccccac acgatcagcg catcgccgcg attggcgcgc
      781 gtcccttcgg cgagctggac cgcttcgatc tcttcgatca gcccagcagc gatgtcgcgt
      841 atctgctgct cgcggagctc gatctcccag cggccgtagc cgcccttctc gagatacgtg
      901 aaccgatata gctgcgagcc cttttcgacg cgcccgccgc gcccgcactc gtgctcgagc
      961 gcgttgtcgc cgaccgcggt cccgatctcg ccgaagtcga ccttcgccgc gacctcgcgc
     1021 atgaaggcac gaaaatcggc cggtatcctg aataccgagc tcacccagag aggatggcat
     1081 cacacgtcgg agggcgagat caatacgtca gaacgcaacg gagtagacga agctcggctt
     1141 cgggtgtcgc aggacacgct cggaaatgac cttgtgcggc accaccgcgt cggaatcgac
     1201 gtagcgcgcg atcagcttct cggccgccgc cttatcgccg cgcgccttga tcccggcaaa
     1261 ctccttcatc atctcctcgg cgaccgccgt gaccttgtcg ggatgcagcg tgaacgcgcc
     1321 cttgtccgtg ccgttcgccg cggtcgcatt cgcgtcccac gtgagcacgc ccttgtcgat
     1381 catgaagccg atctggatcg cggcaagatt gccgtatgcc ttgcggttgt gctcgccggt
     1441 gtacatgccc tgcgagatgt gaccgaacga ccagacgagc atgtcggcga cggtctgctg
     1501 cgcaaactcc tgcgtgatga tgttcttctt tacgaggaag tcgagcagga aatacgcgcc
     1561 ggtctgcgcc tttagctctt ccatcatggc agcgatcggg ccgccaaaca cgtcgtcgtc
     1621 gactttgccc ttcaccttgt actcgtgcgc gggcccgagg ttgtgcgtcg cctcgtgcaa
     1681 gatcgtcgac agcaggccgg gctcggtcga ctcgggatag agcttcaccg tcgccgcgtc
     1741 gagcatcgac atcgcctgct gcttgcgcgc tgcgcggctg tccggatcgg agttgatgtt
     1801 gaccatcgct acggtgcggc cgcggccctc gttcgcaacg ggcccccagt tcggcaagct
     1861 ctgaccgatc gtcgcgccga gcggatcgcg gtcatccgcc gcgttgacca cgatgtcgat
     1921 gaagtcaggc aggtgcacgg cgaccttgcg cgacttgtac ggcgcccccg cgcgcgctgc
     1981 gatcgcgtcc tccatctcct gcagcacggg cgcgagcttg ccctgccagt ctttgctgcc
     2041 ctggttgatc ttcgcgaacg tcagatggaa gccggccttc tgcgcgcagg gctcccagta
     2101 cgtctcatcg ggcgcggcac gcacgtacca ccgcgagttg tcgacggaca tcttcgccca
     2161 cgcttcgtcg gcgggattcc agtcgttcgt cgtgaaccca tcggcggcgg cgttgagata
     2221 cgtgacgagc gccttctcgt tcggatcggt gacggccttc gccgcatcgc gcagcgcctc
     2281 ggcgatcttc gccgtctgct ccttgtaggc gacgttgtat ggaacggctt cgagcgcatc
     2341 gcccgagccg cgcaccacgt tgaagtgccc catcagtgca ttgccgtctt tgcgcgcctg
     2401 caaatcggcg cagaacgtgt ccttcgcctg aatctccgcg ggatagatgt cgaagatttg
     2461 cttcttcagc ccggggatca cgttgcactc ggggttcgcc gtcgtcgtcg ggccgacgca
     2521 cttcgggccg cggttgcgac gaaacaggct ctggctctcg ggatccggcg gcacttcttt
     2581 cgtgatcgcg atgttgccgt tcgtcgtgtc gtagaggttg tcgagcagcg acgcgacgac
     2641 gagcatcttc tcgacgaacg ccttgtcctc ggccgatgcc ttcgtcagat ccgtgaacac
     2701 gagcgacggg cgccccgcat cgagatcttc gatcacgagc tggcgacgct tgtccgacgg
     2761 cggcgcagcc ttcgacgctt cggcgatctt cgcgtacgca tcctcgaagt ccttcgtgaa
     2821 cgcgccgtcc ttcacgtagt tgccctgcgt cggatagaac agcagcggcg cgacctcgtt
     2881 tggatcgagc accttgttgt tgtttgcgtc ggcgctccaa tagatcggca ggttgagatg
     2941 caccgcggtg cggttgaaca ccatgcgatc gacggcatcg tgcgtcgact tgctcggctg
     3001 ggtcatgccg gcggagccgg accccgtcgc gcgcggctgc ggttcctcgc gcttcttgca
     3061 gccaatagtc gcgagcgcga tgacgagata cggagcgatt cgcatgccgc ggacgctagc
     3121 tcagcaacgg caacggcgca acgcgaggcc ccgttccagc gacggcgccg gtggccacgc
     3181 gcatcatcac cgcgcgagca cgcgcgatcg actcggtcgt cacccgccgc ccttcgtcgc
     3241 cctgctccag caccgcgcgc acgacctgct cgagcgcgaa gatcagcgtc tggatcagca
     3301 gcggatcgac gtttccgtgc gacgagctcg cctcgcgaaa catcgagatc agctggtcgt
     3361 ggacttccat gcggcgcgcg tggagcggcg attcgaggct ctgcgcctca ccgccgagga
     3421 cccagataag gcggcccatc tggcgcgtgt tgctgagatg aaggtcgatg cacttctcga
     3481 gctgcttcag gaggtccgat tcgctcgtga tcgcacggcg gcacgcctcg agcaggctgc
     3541 tcgttcccat cgtgtacatc gcgagcgcga cgtcttcctt gctcttgaag aaccgataga
     3601 acgtgcgccg cgacacctgc gacacctcga gcaggtcctc gaccgacacg ttgcgaaagc
     3661 ccttcgtcgc gaacacgcgc gtggcgccga acatgatcat gttgcgcgcg acatcgctgc
     3721 cgagctgaac gccggtggcg cgcttcggca cggtcgtcga ccgcttggcc ttcttgacct
     3781 tcggcgcagc tcgcacgtca ccagtatgcc cgagccgcgg acctaggtat acacggtggc
     3841 tacttggtga acgtcgcctg gaaaaccgcg acgttgtacg gcgagaacga cgacatcgtg
     3901 taggtcaccg tcagctcggt cgcggtttcg ttcagcgtcg ggaacaggta cgtgccgtag
     3961 aagccggtgc ggttgcagtt gaacatctgc ttgccgaggc agttgtcctc gctcgagcag
     4021 cagtacttcg cggtgaaggt agtgtcgccc atgtcatgga cgatgatcgg cgcgtcccac
     4081 gcgtcaggct gcgtcgcgaa tcgcgcgatg atccggttcg aggtcggcgt tagctcttcg
     4141 gcgagtagca tccagcgctc gatcgatggg tagtagtgga cggaaatctc gccatagccg
     4201 gcggtcccga cgatctcgcc gagctcttcg aagcctcccg gttgctcgag ctgggcgagc
     4261 ggcttgcgag ccgcgtagat cttgctcgcg cggtagtcgc cggtgccgaa tatgtagagg
     4321 tagtcgccga cgacttccgc tgcgacattg acgaagtgtc cgtgcagcgg accattcgcg
     4381 tcgaaccgct cgtcgacttg atagaggatc tgatacgcga acgcacccgt cgtggccggc
     4441 tgcgcccact tcgcgaggta gctcgccttc atgtctggat tcgcgcggcc cgtgacggtc
     4501 gtatagaaga cgtagatcgc gccgttcacg ctgaatgcgc cggacggcac ctcgaagtcg
     4561 cccggcagct gcgcgaacgt gttgccgtcg gcgccggctg gattgcggat gtattcgcca
     4621 agcccatgcc ctgacggtgc gatcatcgca gcgcccgcga agtcggcctt gaccgctgca
     4681 ttcacggacg ggccgatgct ctgtgccgcc ggcagcgaca cgatgcgcat gcgttcgcac
     4741 agcagcgcgg gattcgccgc gatcgcggtc gcggaatcga ggccgtagcc gacggaatcg
     4801 ggatgcgact cgttgatcgg ccagatgccg gcgaagccga tcgtgtcgcc gaacaagata
     4861 tagagcttgt cgttcgcggc gaccggaatg ccgaggtctg cgccgagcac gttcgagttg
     4921 tgctggacgt cgttcgccga tggatcgttg acgttgcggt ccgagagcag cttgcacagc
     4981 tgctgcgtgc tcgcgagcgt tggcgcgaag tgcgcatcga cggtcgagct cgatgaatcc
     5041 gccgctgcgt cgggatcgcc ggcatcgggc gcgttgccgc tgcaaccgag cacgaacaag
     5101 aggagcgaga gacgacgcga catgatcaat tcatcgtcac ggtggcgatg tgcgtcgcag
     5161 catcccacgt gatcgctccg tgctcgaagt cagatcgacg gcctcccgcg acgtcgtgct
     5221 cgtctgacac tggataaccg agcgggcccg cttcccaacc ggtctccatg tacttgtcac
     5281 gaatggcacc gtgcacctcg aacgcgccgg tgaacggcga ccagtatatg gagccatttg
     5341 cgaacacgtt gtagcgaccc gctccgtcag gtgtcgtcag ctcgtcggtg agcgcgggcc
     5401 cgagaaagct cccgcagccg ccgagcgctt gatagtgcgc gttgatgtcg ccgatcgtgc
     5461 ggcccacgcc gcagcgatac gtcgcgtagc ccttcggtgg gatcagcgtc gcagcttgat
     5521 cgaacgacag ccagtagtgc tcgatgcccg agaagcgcgc cgggtctgcg acgtagacct
     5581 gcttggtgtc cgcgttgtat ccgatgagag agatgtagtg ataaatcgtg tagccgggat
     5641 agccgggcgg atgattgctc gccggggcta cgatgttcgc gacgatgccg tggcccgcat
     5701 cgatcgacag gcggatgtcg cgccacagtt gatcgcgctc ctgcgccgtc ggcggatcat
     5761 gcggcatcag cacggcggca tatcgcgcac cgacgtgctg gttgagcgcg ttgacgacga
     5821 ggccgatgtg atcggttccg ttcgtcgagg tcggtagctc gttcgcgagc atctgctgag
     5881 tcggcaggtt cgcgctaatc gtggacagcg ccatgcgtgt cgatgcggga ccgcaccaat
     5941 agccggtctg ctgtgcctga tactggtgtt cgaggacctt ccacggctgc tgcgcctcga
     6001 gcgtcagctc gctcgcgctc cccgagcccg accgggtgcc ctcctcggta atagggtccg
     6061 tgtccggaac ctcggtgccg gacacatccg caacgcacgc agtgagaaac aagaatgtga
     6121 agcgatacat gacgtctcct tagtaagtcg cggttgcagt gtcggtttgc gcgatccagg
     6181 tgatcgagcc gtgctcgaag tcgctgcggc gtccgccggg aacggcgtac tcgtcgctcg
     6241 tcggatagcc gagaggtccc gcctcccagc cctcgtcttt ccacttgtcg cgaatgcggc
     6301 cgtgcacctc gtacgcgccg atctgcgggg tccagtagat cgagccgcgc tcgaacacgc
     6361 tgtagcgacc gatgccgtcg ggcgtacccg cctcggcggt gatcggcgca ccgacgatcg
     6421 aagcgcagcc gccgagtgca
//
LOCUS       DATLBZ010000656         9273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_11088806, whole genome shotgun sequence.
ACCESSION   DATLBZ010000656 DATLBZ010000000
VERSION     DATLBZ010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11088806"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1471)
                     /locus_tag="VMZ53_32215"
     CDS             complement(185..1471)
                     /locus_tag="VMZ53_32215"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33227.1"
                     /translation="MKVQRDAVAQHTQVDRALRRSAVAHAALDADEAHWLRIAEEQRI
                     WAKLGYVHGFEYLEDVFGYAPRTASERLRVARELGELPQLEDALRCGEMSWSVVRELT
                     RVATPATVDCWIEDARGRRLRDVEQMVAGRKKGDEPGAPPDPKRVKHRVVLELDGESL
                     AIWRQMHQAVEIESDCKLDDRAFVLALGTRVLTEPHEAGGSASAPKPPASSAIGRHTI
                     HVITCRDCGRGWQDGAGVRIEVESSVIERAQCNAIIVDDEKGKRPTRTLPARTERQVL
                     ERAQHRCEVSGCRSSKHLAIHHIKFWANGGTHDLWNLIALCDGHHRVLHDGLLTITGK
                     APDELVFERNGVPLVRREGHAPRKHGGAAHVKKAMNAPNLEKDDPVALAQAALRQLGF
                     KAPVAARAVDMACAHVGTNVELGELIKEALRQCAER"
     gene            2440..2790
                     /locus_tag="VMZ53_32220"
     CDS             2440..2790
                     /locus_tag="VMZ53_32220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33228.1"
                     /translation="MRSIIVVAAALAGCMTIDSKPIEMPMPTPAERVWQDNAFPMLVQ
                     QCAECHRYNQVGFLDGNNLWEVRETLVTSTVVDLQEPTKSRLLVMGVHSGPALTAEQT
                     STLLEWLTAERAER"
     gene            complement(2802..3245)
                     /locus_tag="VMZ53_32225"
     CDS             complement(2802..3245)
                     /locus_tag="VMZ53_32225"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HUS33229.1"
                     /translation="MPTTSRKAPPLFTVGYEGRALDELLAILSAESIDCVIDIRELPL
                     SRRRGFSKTPLGTALRSVGIDYVHMRSAGNPYRREKDSIPRDKLLAKYAKHIAKAEPI
                     VDDVVKVARGKRAALLCYEHEPADCHRSLLAPRVAAKLKTKVHDL"
     gene            complement(3272..4648)
                     /locus_tag="VMZ53_32230"
     CDS             complement(3272..4648)
                     /locus_tag="VMZ53_32230"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS33230.1"
                     /translation="MGAADRPRVLVVDKSSTTRELIAELLGDTARELAWASRDAVLDK
                     LRAAATAGQGFDVIVLDGDGATPVLVRELSTAARGARVVLIANGVEGVALANRCGATD
                     VVSRPFSGPELQFRIGRASGAASGEREAKRWVRKSDVLIGSGAWIKELYDRIAMVAAT
                     DVNVAIFGESGTGKELVARTIHSSSPRHEAPFVVVNCAAIPENLIEDELFGHVRGAFT
                     DATRDREGLLAAAHTGTLFLDEIGELPLLLQAKLLRVLQSQEFRRIGEDVDRRVDVRI
                     VTATNRDLDQLVARGAFRQDLYYRINVFPMHMPPLRERPEDLPLLVHHFVQKYRGRLG
                     KTIEGITPAALTRLAAYDFPGNVRELENKIHQAMVVAPGTQIDEDDLALPAPSPTSSV
                     RVDVTRPFRDVKQETIDAFERAYLTELLRAHRGNLAQAARAAGMDRKNLWALVERHGL
                     DRARFKKP"
     gene            complement(4816..5676)
                     /locus_tag="VMZ53_32235"
     CDS             complement(4816..5676)
                     /locus_tag="VMZ53_32235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33231.1"
                     /translation="MRRGAWSALLLLALAGWEPFRAPNPEVEAGNKALAEGKLDDALA
                     HYNKAGHDADVDPNGLAYNKGSAELQQAKGATDPKEKERLTQRGLDDLKQAATSSDPK
                     IRAQANFNRGNALLDGNKLEESIESYKNALRDAPNLEDARVNLELALKRRQKQQQQQQ
                     GQGKGQGQGQGQGQGQQGQQGQQQGQQGQQGQQQGQQGQNQQQQQQQQGQGNGQQQQN
                     QQQGQGQKDQQKQNQQQQKPRKDHTQPQSPQTPTDGKLDDLESFSRNLQKDSARRRAS
                     GRAKDPDKDW"
     gene            complement(5700..6884)
                     /locus_tag="VMZ53_32240"
     CDS             complement(5700..6884)
                     /locus_tag="VMZ53_32240"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS33232.1"
                     /translation="MSFAYREFLYAAAAMPVLLLLIFLYDRMQRRRLQNRLGELPVLE
                     KVMASSSPRRRWIKLVLVALGMSLLLVASARPQIAGKRKVELRGLDLVVAVDVSKSML
                     VDDVGQTLLMQQKRTPASRLARARELAAAMIDEVPDDRIGPVVFAGAASHFPLTDDHE
                     VAIRFLYDLGPADLPPGSNLTEVFRVSRCLLRPDLYDDLGCSRIGRRGHGGDPLPGDS
                     EGLDPEKKPGDDDIVEEKAERGKAMVIFTDGGESDQEALREVATSRELGIAVFVVGLG
                     TTQGGVVYEVDYQGNKTATPKLDSAGNTVISKRDDAGMKLLAEAGGDPNRFFVASETG
                     EVDPTPIVESLRAVNRGLATKQVDEMKDIFEPFLFAGFMLLVIEAAISTRRRRKYPEE
                     HA"
     gene            complement(6881..8044)
                     /locus_tag="VMZ53_32245"
     CDS             complement(6881..8044)
                     /locus_tag="VMZ53_32245"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS33233.1"
                     /translation="MSEASEQTKTFTPERVFWIVWPWIMTFVIGAFLLGVASAVFDVP
                     VARMWAELKYALHLSTQQHPERANEMRQVVWKHPHALVLALGSGLVGLIGFHLHRRRS
                     AAIGFSNVKLVKTRGLGAYFAPLPTALRVLAVATLAIGLGRPETYRIVKHQVDSVDIM
                     LVMDMSKSMEETDMLRDRMDAAQRVIRRFLRRNKNDRIGLVIFGQQAMLQCPLTHDTK
                     ILEKIVSDLVIGDVPELGTAIGDGLALAVAQLRRSDAKSKVVILLSDGDSNWVTRFDP
                     DEAARVAQEQKIKVYTLLVGREESDLFGGMSVNPATLRNIATRTGGEFFRASDYASFD
                     RGFQTVRNQLDTTKRTISERVPDKQLFVPFALIAAALVALEFLLAHTRLRRLP"
     gene            complement(8041..>9273)
                     /locus_tag="VMZ53_32250"
     CDS             complement(8041..>9273)
                     /locus_tag="VMZ53_32250"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HUS33234.1"
                     /translation="GAGGAMGGGGASGGGAMGGGGAMGGGGASGGGAMGSGGAMGGGG
                     AGGAMGSGGAGGAMGSGAGSGSAIVIIIPPDVQAPDVSAAASPSVVRLGERFTLFITA
                     RHTRDVEVNLREPVELGGSFEVRKKVSEDRLNADGSKTREWQLDVYAWDVGELRVPPI
                     AVTFTAGGKAGQVATNSVPINVTGVLGDVIDDPKLMRGNAPPVTLLSRDWFWAWVAGG
                     AALLLAGVIAFVIIRKRRKKPVRSLIGTLIASTPTPRRLDMTSERALAQLLAIEKSGV
                     LDKDDQRKQGYLEMVDVIRDYVGARYRIQTTDLTSYELIRALRHAAPEHEQKLVEEWL
                     DRCDIVRYGGFKATSDDAYGVLEAARHLVITTTRAPGASASNSSEAASSSGRFPPLSD
                     ADKQSADAAKASSDKEAP"
BASE COUNT         1440 a         3062 c         3111 g         1660 t
ORIGIN      
        1 ggccttgcga ctgcgctgga cggattgaag gatccgcggg ctgcctcgac atggcagcgg
       61 ttcgagaagg cgtgggctcg cagcgacatc aagctgacgt cgtcggtgct ttgatcgtca
      121 tcgcgtgggc gtgtgatggt cgccgccacg aagacgcgtt ctgcccacgt gggcgcgatg
      181 agcctcagcg ctcagcgcac tgccgtaggg cctctttgat cagctcgccg agctcgacat
      241 tcgtgcccac gtgggcacac gccatatcga ccgcgcgcgc cgcgaccggt gccttgaagc
      301 cgagctggcg aagggcggcc tgtgcgagcg caacgggatc atctttctcg aggttcggcg
      361 cgttcatcgc tttcttcacg tgtgccgctc cgccgtgctt tcgcggcgca tgcccctcac
      421 gccgcacgag tggcacgccg ttgcgttcga agacgagctc gtccggcgct ttgccagtga
      481 tcgtcaagag gccgtcgtgc aagacgcgat gatggccatc gcagagggcg atcaagttcc
      541 agaggtcgtg cgtcccgccg ttagcccaaa acttgatgtg atgaatcgct aggtgcttcg
      601 acgagcggca gcctgacacc tcgcaacgat gctgcgcacg ctcgagcacc tggcgctcgg
      661 tgcgtgctgg aagtgtccgc gtcgggcgct tccccttttc gtcgtcgacg atgatcgcgt
      721 tgcactgcgc gcgctcgatt acggagctct cgacttcaat gcgcacgcct gcaccgtctt
      781 gccatccgcg tccgcaatcg cggcacgtga tcacgtgaat cgtgtggcgg ccgatagcgc
      841 tggaagcggg tggcttggga gccgacgccg agccgcccgc ttcatgcggc tcggtcaaga
      901 cgcgcgtgcc gagcgcgagc acgaacgcac gatcatccaa cttgcagtcg ctctcgattt
      961 cgacggcctg gtgcatctgc cgccagatcg ccaagctctc cccatcgagc tcgagcacca
     1021 ctcgatgctt cacgcgcttc ggatcaggcg gagcgcccgg ctcgtcgcct ttcttgcggc
     1081 ccgcgaccat ttgctcgaca tcgcgcaacc tccgcccgcg cgcgtcttcg atccagcaat
     1141 caacggttgc gggcgtcgcc acacgcgtca gctcgcgcac gaccgaccag ctcatctcgc
     1201 cacatcgcaa cgcgtcttcg agctggggca actcgccgag ctcgcgggca acacgaagcc
     1261 tctccgacgc agttcgcggc gcataaccaa agacgtcctc caaatactcg aagccgtgca
     1321 cgtagccgag cttcgcccaa atgcgttgct cctcggcgat tcggagccaa tgcgcttcgt
     1381 ccgcgtcgag cgccgcatgc gcgacggcac tgcgccgcag cgctcgatcg acctgcgtgt
     1441 gttgcgcgac cgcgtctcgt tggaccttca ccaatgtatc gcgtgacatg aacgttcact
     1501 accacgggag attttggctt cgctgtttgc gctctgcgcg cctatcggca gctttctcga
     1561 cgagaaacga ctcgcgtcgg atccgatgca tcacgtcgca cggagaacgc gcacacgcgc
     1621 gatcgtttgg ggtttgtcgt cgagtacgcg cggggcgccg cgaatcgcgt caacaagaaa
     1681 gttgtcaaac attgtcgtcg aaagaacgca tcgacagtcg cgccgcctcg tgcgcacgac
     1741 cgccgatgcg ttgcgcgaac gtcatgccca cgtgggcaca tcgcatcgac gatcgcgtcc
     1801 acgagcgcgc atcgcatcga cgatcgcacc cggcacatcg cgaacacgcc ctcgtcagcg
     1861 gttcgcgact agtgctgcga cgtgatcgga gaagaagtga acgagtagcg agcgtgccgc
     1921 gcctgggccg gcggcgagtc gcggcgcgcg cccctggccg tttgaaaggc cggggcgcgc
     1981 cgcgtcccgc cggcccggcg ctcccagcag cggcgcagaa gaagaacgca cgcctcatcg
     2041 tgacgatcgc gcttcccgcc cacgtgcgca catcgcagcg aacgccgcgt cggcagatcg
     2101 acgagctcgg ccaatcgcat cgacgaacgc gcccacgagc gcgcatcgca tcgacgatcg
     2161 cacccggcac atcgagaaca cgccctcgtc agcggttcgc gactagtgct gcgacgtgat
     2221 cggagaagaa gtgaacgagt agcgagcgtg ccgcgcctgg gccggcggcg ctccgagcag
     2281 cctcggagca gaagaaggca cgttacttcg tgacgatcgc gcttcactcc ctcgtgggca
     2341 catcgcatcg aacgtcgcgc ccacgtgggc attcgcagct ggtggctgcc atgtggtcgg
     2401 ggaaggttgg cgtgcgcggt gctgtggtag ggtccgcgca tgcgttcgat catcgtcgtg
     2461 gcggcggcac tggcgggttg tatgacgatc gacagcaagc cgatcgagat gccgatgccg
     2521 acaccggcgg agcgcgtctg gcaggacaat gcgtttccga tgctcgtgca gcagtgcgcc
     2581 gagtgccatc gctacaacca ggtcggattt ctcgacggca acaatctgtg ggaggtccgc
     2641 gagacgctcg tgacctcgac ggtcgtcgac ctccaggagc ccacgaagtc gcgcctgctc
     2701 gtcatgggcg tgcattcggg tccggcgctg acggcagagc agacctcgac gctgctcgag
     2761 tggctgaccg ccgagcgcgc cgagcgctag cgagccagcg gctagaggtc gtggaccttc
     2821 gtctttagct tcgcggccac gcgcggcgct agcagcgagc gatggcagtc ggccggctcg
     2881 tgctcgtagc acaggagcgc tgcgcgcttg ccacgcgcga ctttgacgac gtcatcgacg
     2941 atgggttcgg ccttcgcgat gtgcttggcg tacttcgcga gcagcttgtc gcgcgggatc
     3001 gagtccttct cgcggcgata cgggttgccc gctgagcgca tgtggacgta gtcgatgcct
     3061 acgctgcgga gcgccgtgcc taggggcgtc ttcgagaagc cgcggcggcg agagagtggc
     3121 agctcgcgaa tatcgatcac gcaatcgatc gattcggcgg agaggatcgc gagcagctcg
     3181 tcgagggcgc ggccttcgta gccgaccgtg aagagcggcg gcgccttgcg cgacgtcgtg
     3241 ggcacacgag cggtttagca gggagcggcg gctagggctt cttgaagcgc gcgcgatcga
     3301 ggccgtggcg ttcgacgagc gcccacaggt ttttccgatc catgcctgcg gcgcgtgcgg
     3361 cttgcgcgag gttgccgcga tgcgcgcgca ggagctccgt gagataggcg cgctcgaacg
     3421 cgtcgatggt ttcttgcttc acgtcgcgga agggacgcgt cacgtcgacg cgcacgctgc
     3481 tcgtcgggct gggcgccggg agggcgaggt cgtcttcgtc gatttgtgtg cccggggcga
     3541 cgaccattgc ttgatggatc ttgttctcga gctcgcggac gttgcctggg aagtcgtacg
     3601 cggcgagccg cgtcagggct gcgggcgtga tgccttcgat cgtcttgccc aggcgcccgc
     3661 gatacttctg cacgaagtgg tggacgagga gtggcagatc ctctggacgc tcgcgcagtg
     3721 gcggcatgtg catcggaaag acgttgatgc ggtagtagag gtcctggcgg aaggcaccgc
     3781 gtgcgacgag ctgatcgagg tcgcggttcg tggcggtgac gatgcgcacg tcgacgcggc
     3841 gatcgacgtc ctcgccgatg cggcggaact cctgcgactg caggacgcgc aggagcttgg
     3901 cctgcagtag cagcggcagc tcgccgatct cgtcgaggaa aagggtgccg gtgtgggcgg
     3961 cggcgaggag gccttcgcga tcgcgcgttg catcggtgaa ggcgccgcgc acgtggccga
     4021 atagctcgtc ctcgatcaag ttctcgggaa tcgccgcgca gttgacgacg acgaacggtg
     4081 cttcgtgccg cggcgaggag ctgtgaatcg tgcgcgcgac gagctccttg cccgtgccgc
     4141 tctcgccaaa gatcgcgacg ttgacgtctg tggccgcgac catcgcgata cggtcgtaga
     4201 gctctttgat ccacgcgccc gatccgatga gcacatcgct cttgcggacc cagcgcttcg
     4261 cctcgcgctc gccgcttgcc gcgcccgatg cgcgaccgat tcggaattgc agctcgggtc
     4321 cggagaacgg tcgtgagacg acgtcggttg caccgcaacg attggcgagc gccacgccct
     4381 cgacgccgtt cgcgatgaga acgacgcgcg cgccacgcgc cgcagtggag agctcgcgga
     4441 cgagcacggg ggtggcgccg tcgccatcga gcacgatgac gtcgaagccc tgccccgcgg
     4501 tcgcggccgc gcggagctta tcgaggacgg catcgcgcga tgcccacgcg agctcgcgcg
     4561 ccgtatcgcc gagcagctcc gcgatcagct cgcgtgtcgt cgatgatttg tcgacgacga
     4621 gaacgcgcgg acgatcagcg gcccccacgc cccgagtcta cccgctgcgg cgaacgtgtg
     4681 cgtatcgggt ctgctgcggc gaattaccga ggggaaacgt agggcaggac gcgacctcgt
     4741 gctcgggacg gaccgacgag ccagacgaag cctttgtcgg gacctccgcg gccactcgcg
     4801 agccggcggt cgccgctacc agtctttgtc gggatctttc gcgcggccac tcgcgcggcg
     4861 gcgcgccgag tctttctgga ggttgcgcga gaaggactcg aggtcgtcga gcttgccgtc
     4921 ggtcggcgtc tgcgggctct gcggctgcgt gtggtctttg cgcggctttt gctgctgctg
     4981 gttttgcttc tgctgatctt tttggccttg gccctgctgc tggttctgct gctgctggcc
     5041 gttgccttgc ccttgttgtt gttgttgctg ttgctggttt tgaccttgtt ggccctgctg
     5101 ctggccctgt tggccttgct gaccttgctg ctggccttgt tggccctgct gaccttggcc
     5161 ttggccttgg ccctgtccct ggcctttgcc ttggccctgt tgctgttgtt gctgtttttg
     5221 gcggcgcttg agcgcgagct cgaggttgac gcgcgcgtcc tcgaggttcg gcgcgtcgcg
     5281 aagcgcgttc ttgtacgact cgatcgactc ttcgagcttg ttgccatcga ggagcgcgtt
     5341 gccgcggttg aagtttgctt gcgcgcggat cttggggtcg ctggacgtcg cggcttgctt
     5401 gaggtcgtcg aggccgcgtt gggtcaggcg ctctttctct tttggatcgg tcgcgccctt
     5461 tgcttgttgg agctcggcgc tgcctttgtt gtaggcgagg ccgttcggat cgacgtcggc
     5521 gtcgtggccg gctttgttgt agtgggcgag cgcgtcgtcg agcttgcctt cggcgagcgc
     5581 tttgttgccg gcttctacct cggggttcgg cgcgcgaaac ggctcccagc ccgcgagggc
     5641 gagcagtagg agggccgacc aggcgccgcg acgcatgcct cgatgatgac atgggcggct
     5701 cacgcgtgct cctccggata tttgcgacga cgacgtgtcg aaatggcggc ctcgatgacg
     5761 agcagcatga agccggcgaa caggaacggc tcgaagatgt ccttcatctc gtcgacttgc
     5821 ttcgttgcca ggccgcggtt cacggcgcgc agcgattcga cgatcggggt tggatcgacc
     5881 tcgccggtct cggaggcgac gaagaagcgg ttgggatcgc cgcccgcttc cgcgagcaac
     5941 ttcatccctg cgtcgtcacg cttggagatg accgtgttgc cggcgctgtc gagcttcggt
     6001 gtcgcggtct tgttgccttg atagtcgact tcgtagacga cgccgccttg cgtcgtgccg
     6061 aggccgacga cgaacaccgc gatgccgagc tcgcgcgacg tcgcgacttc gcgcagcgct
     6121 tcttggtcgg actcgccgcc gtcggtgaag atcaccatcg ctttgccgcg ctcggccttc
     6181 tcttcgacga tgtcgtcatc gccgggcttc ttctcgggat cgaggccttc actgtcgccg
     6241 ggaagcggat cgccgccgtg gccgcgccgc ccaatgcgcg agcagccgag gtcgtcgtag
     6301 agatcggggc gcagcaggca ccgcgagacg cggaagactt cggtcaggtt cgagcccggc
     6361 gggagatcgg cggggccgag atcgtagagg aagcggattg cgacctcgtg gtcgtccgtc
     6421 agtgggaagt gggaggcagc gccggcgaat acgaccgggc cgatgcgatc gtcgggaacc
     6481 tcgtcgatca tcgcggctgc gagctcgcgg gcgcgggcca ggcgcgacgc gggcgtccgc
     6541 ttctgctgca tgagcagcgt ttggccgacg tcgtcgacga gcatcgactt cgacacatcg
     6601 accgcgacga ccagatcgag gccgcgcagc tcgaccttgc gcttgcctgc gatctgcggc
     6661 cgcgccgacg cgacgagaag cagcgacatg ccgagcgcca cgagcacgag cttgatccag
     6721 cgccgccgtg gcgacgacga ggccatcacc ttctcgagca cgggcagctc gccgagccga
     6781 ttctgcaggc gccgccgctg catccgatcg tagaggaaga tcagcagcag caggaccggc
     6841 atcgcggccg ccgcgtagag aaactcgcga tacgcgaagc tcatggcagc ctccgcaggc
     6901 gcgtgtgcgc gagcaggaac tccagcgcga cgagcgcggc ggcgatcagc gcgaacggca
     6961 cgaacaactg cttgtcaggc acgcgctcgc tgatcgtgcg cttcgtcgta tcgagttggt
     7021 tgcgcaccgt ctgaaagccg cgatcgaacg atgcgtagtc ggacgcgcgg aagaactcgc
     7081 cgccggtgcg cgtcgcgatg ttgcgcagcg tcgctgggtt cacgctcatg ccgccaaaca
     7141 agtcggactc ttcgcgcccg acgagcagcg tgtagacctt gatcttctgc tcctgcgcga
     7201 cgcgcgccgc ctcgtcgggg tcgaagcgcg tgacccagtt cgagtcgccg tcggagagca
     7261 ggataacgac cttgctcttc gcgtcggacc gacgcagctg cgcgaccgcc agcgcgaggc
     7321 catcgccgat cgccgtgccg agctcgggca catcgccgat cacgaggtcg ctcacgatct
     7381 tctcgaggat cttcgtgtcg tgcgtgagcg ggcactgcag cattgcctgc tggccgaaga
     7441 tcacgaggcc gatgcgatcg ttcttgtttc gccgcaggaa gcgccgaatc acgcgctgcg
     7501 ccgcatccat gcgatcgcgc aacatgtcgg tctcttccat cgacttcgac atgtccatca
     7561 cgagcatgat gtcgacggaa tcgacctgat gcttgacgat gcgatacgtc tcgggccggc
     7621 caagcccgat ggcgagcgtc gcgacggcga gcacgcgcag agcggtcggc agcggcgcga
     7681 agtacgcacc gagcccgcgc gtcttgacga gcttgacgtt cgaaaagccg atcgcggcgc
     7741 tgcgccgccg atgcaaatga aagccgatga gcccgaccag gccgctgccc agcgcgagca
     7801 cgagcgcgtg cggatgcttc cacacgacct ggcgcatctc gttcgcgcgc tctggatgct
     7861 gctgcgtcga gagatgcagc gcgtacttca gctcggccca catgcgggcg accggaacgt
     7921 cgaacaccgc cgacgcgacg cccagcagga acgcaccgat cacgaacgtc atgatccacg
     7981 gccacacgat ccagaacacg cgctccggcg tgaacgtttt cgtctgctcg ctcgcctcgc
     8041 tcacggcgcc tccttgtcgg agctcgcctt ggccgcgtcg gcgctctgct tgtcggcatc
     8101 ggagagcggc ggaaagcgtc cagagctcga cgccgcctcc gacgaattcg acgcagatgc
     8161 gcccggcgcg cgcgtcgtcg tgatgaccag atgtcgcgcg gcttcgagca cgccatacgc
     8221 gtcatcggac gtcgccttga agccgccgta ccgaacgatg tcgcagcgat cgagccattc
     8281 ctcgacgagc ttttgctcgt gctcgggcgc ggcatggcgc agcgcgcgga tcagctcgta
     8341 gctcgtgaga tcggtcgtct ggatgcgata gcgcgccccg acgtagtcgc gaatgacgtc
     8401 gaccatctcg aggtagccct gcttgcgctg gtcatctttg tcgagcacgc ccgacttctc
     8461 gatcgcaagc aactgcgcga gcgcgcgctc actcgtcata tcgaggcggc gtggcgtcgg
     8521 tgtcgaggcg atgagcgtgc cgatgagcga gcgcacgggc ttcttgcgtc gcttgcggat
     8581 gatcacgaac gcgatcacac ccgcgagcag cagcgccgcg ccgcccgcga cccacgccca
     8641 aaaccagtct cgcgacagca gcgtcacggg tggcgcattg ccgcgcatga gcttcggatc
     8701 gtcgatcacg tcgccgagca caccggtcac gttgatgggc accgagttcg tcgcgacctg
     8761 cccagcctta ccgcccgctg tgaacgtcac ggcgatcggc ggcacgcgca gctcgccgac
     8821 gtcccacgca tagacatcga gctgccactc gcgcgtcttg cttccgtcgg cgttgagccg
     8881 atcttcggac accttcttgc gcacttcgaa cgagccgccg agctcgacgg gctcgcgcag
     8941 gttcacctcg acatcgcgcg tgtggcgcgc ggtgatgaac agcgtgaagc gctcgccgag
     9001 ccgcaccacg ctcgggctcg ccgcggcgct cacatcgggc gcctgcacat cgggaggaat
     9061 gatgatcacg atggcggagc ccgacccggc ccccgacccc atcgcaccgc ccgcaccgcc
     9121 cgaccccatc gcaccgcccg caccgccgcc gcccatcgca ccgcctgagc ccatcgcacc
     9181 gccgccgctc gcaccgccgc cgcccatcgc tccgccgccg cccatcgcac cgccgccgct
     9241 cgcaccgccg ccgcccatcg caccgccggc acc
//
LOCUS       DATLBZ010000657        29836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8862116, whole genome shotgun sequence.
ACCESSION   DATLBZ010000657 DATLBZ010000000
VERSION     DATLBZ010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 29836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29836
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8862116"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VMZ53_32255"
     CDS             complement(<1..635)
                     /locus_tag="VMZ53_32255"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HUS33235.1"
                     /translation="MNRELASTLARLALSAVERPLPHKLDHVVTREGERASHRELHPA
                     FYGAFDWHSAVHGHWTLARVLALAPDVAEASEIKRVLDAHLTVENLRREAEYCAAHPA
                     FERMYGWAWTLELARVLRGTPWEEAIAPLADVIAGHYLAFLPKQAYPIRTGTHTNTAF
                     GLLFAFDYAQEVKQHALANLIEDRCRSYFARDVDAPAIWEPGGDDFLSPSLV"
     gene            complement(632..1252)
                     /locus_tag="VMZ53_32260"
     CDS             complement(632..1252)
                     /locus_tag="VMZ53_32260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HUS33236.1"
                     /translation="MPEKRYLVKEMFGPTLQGEGAHAGRPCVFLRFAACNLECPWCDT
                     DFAPDGAVRYDASEIVSRLLELDVHRARMVVVTGGEPTLQWDQPLSDAIHAADFRIHM
                     ESNGTRVPNGTVDWLTVSPKPQFHPTSITLANDVPVSELKVVVDDTVDAAVLDRYAEK
                     YRCEHYFAQPCMNDEYPRHLARTIELVQSRPRWRLSVQMHKVIGVP"
     gene            1282..2163
                     /locus_tag="VMZ53_32265"
     CDS             1282..2163
                     /locus_tag="VMZ53_32265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33237.1"
                     /translation="MNRCVVLAALVCAAACGKRQQVESGVGSGVGSDTCEQLPFAASV
                     PVAEASGAAWLEVGGKQALVVNADSGHRGEYAIIDPETGNVIEQGSLPLGQVPKDADD
                     IEGLATRGGMLYGLQSSGWMRVWKRVGSGFELVDGPYPIATGDNFSCAIDDGNCGKNY
                     EGLAIAPKPNGPCAGFACSKEDGHLYCLVEQQDGRFAVDRTKSIAVERKGVLADCAFS
                     PLGKLFVGNNMFGNSTVYVVDGWADPAKARVEEVGELGVGFPEAIAVDSALIYRLSDT
                     GGSPSMMAKFRCLAPGR"
     gene            2173..2649
                     /locus_tag="VMZ53_32270"
     CDS             2173..2649
                     /locus_tag="VMZ53_32270"
                     /inference="COORDINATES: protein motif:HMM:NF015442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HUS33238.1"
                     /translation="MQCPYCSGDSSVTETRVTADGMRRRRVCTACKRRFTTYEKLGAP
                     LLKVAKRDGSVEPFDGEKLFHALQRVAAHRDISDEDLRRIARDIEATLVDSSRKSVTW
                     NDIVSLVLDRLRNVDPITAHRFAANYTDETGVLRFENDDRPAGDPRQLGLPLTEED"
     gene            complement(2651..2872)
                     /locus_tag="VMZ53_32275"
     CDS             complement(2651..2872)
                     /locus_tag="VMZ53_32275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33239.1"
                     /translation="MADDPFKTIDLGALEEVTGGRYTRGADAIDPQLIQGIGELAKAV
                     TAVGQGLAATKQQSDGQMMQLLQQMMQKR"
     gene            complement(2884..3189)
                     /locus_tag="VMZ53_32280"
     CDS             complement(2884..3189)
                     /locus_tag="VMZ53_32280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33240.1"
                     /translation="MSKKTKFQKIEDAALEKVQGGAARVAATSKSSDLDSKLTTMLSQ
                     IGDSIKEVAKPQQDNSMTTMMMMMMMMGGGGGGGSAPAAAAPPPPQGGTYVKVNVRR"
     gene            complement(3300..3545)
                     /locus_tag="VMZ53_32285"
     CDS             complement(3300..3545)
                     /locus_tag="VMZ53_32285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33241.1"
                     /translation="MYDLDAIETINAEQLVTPLDRILLAGGRPRRRPSRPTIDMTAAA
                     EKTLFIRTQRTTHRSSHLIAAAMCVTWFSVAVACLLA"
     gene            complement(3780..4109)
                     /locus_tag="VMZ53_32290"
     CDS             complement(3780..4109)
                     /locus_tag="VMZ53_32290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33242.1"
                     /translation="MSNSKDPFVSITTDDLDKVNGGAARVAASGGGSDTNDQLMLMLT
                     SIGDSIKDLAKNNNSGGGDMTTMMMMMMMMGGMGGGGGGAVAAPAPQQPTYVEVSAKG
                     GCGKKGW"
     gene            complement(4360..5937)
                     /locus_tag="VMZ53_32295"
     CDS             complement(4360..5937)
                     /locus_tag="VMZ53_32295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33243.1"
                     /translation="MFNFKVLALLAASGAATLLAASDADAKPRRVVVLDFDGPRQLAD
                     NGRSAVMSVLGDQYNIVATKNWDTARARSSGRGPQQWRQASKQAGVDAVIEGWVQTEG
                     RHHVLTVAVRDASTGNEIDTLSVRIKDNGVTTEAGHKLAAQLDEVLAWIDGDITAEPT
                     QSLPEYRPPLLGAKDPSKDRERSRDEQDDEDDAELTDDDTVRSRHKKHKKRKNRVAEE
                     QPADEAVSDEEEAAPSKTKAEEKQVAVADPIATKDTNDLVTLFGPESKEAEIVTEGKS
                     KHVPVPTPRFSINAGPYLSSRGLNFTNDPEAKESPPSYPASSLRGFAAQIEVFPMPTQ
                     KVDGRLSGLGFSFDVKRSVGSVLTVMDDTAYGDYDISHVAWQTGIHYRYPIDIVSIDA
                     SADFGNVSHSLPGDFPQSVAIPDTSFSYLGGGVHVDLQVTEKASVGAGARYMYLLGAG
                     MVTEQDWYGAGKANGVAFDGNFSIPLPHNLYVKGGIEYRRIKIDFEGSGELSQAWGVW
                     DVTDAAVTGTGTVGVQF"
     gene            complement(5961..6959)
                     /locus_tag="VMZ53_32300"
     CDS             complement(5961..6959)
                     /locus_tag="VMZ53_32300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33244.1"
                     /translation="MRTTNLALVSLLSACVAPIGDRDHDNFGPDAGVDGSSATGACDD
                     LKIVTMNLTVSGASNFNNLPTTCWKLNGKLVITGPAITSVAKLGDLREVTDLEINDAD
                     IAKFDTKAAVDVSGDIYIHNNDKLTDIANIVAKSTVKSIRVEYNPLLPNLGGLNRASI
                     VSGATSITNNLKLTAVDFTSMQRLEGGVTISDNTVLTSIQLSSLQSVGNVSISRNPAL
                     TTLSNMSSMTNVHGAFSIDDNDALVSLGQFGSSITVDTNVTIANNAKLTDTGAIDHAT
                     RIFGTVAINNNAQLDVSKAHDIGCCVATGAFTATGNKTNQCNGNHYCLNNQQNCYR"
     gene            complement(7078..8697)
                     /locus_tag="VMZ53_32305"
     CDS             complement(7078..8697)
                     /locus_tag="VMZ53_32305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HUS33245.1"
                     /translation="MRSIVIGTILLATAASASARPRHSRVAQNEEEQPQRDEEDKDGT
                     EAVSALPIKIENLIEAAVARAPDLVRARIDRVAAGAAAEGSRRDQAWIASASTEAKRS
                     ALAEDVEAPPFSVVANNEVYGGVGLGRNLPTGGNIQLEAGFDRKVTEYNIVSRLHQAK
                     EQQQAPAGTDANDNPYDMLQRNTAILKATFKQPLVRGFGPAVALAPQKRADLQATEAT
                     VKAQLAGEQLIRDLINDYWDLALASYELDVRNQSLELAKKQEDITHDLIRAGTVPSTA
                     ANAVVYEIQIRQEAALRAQLALEQKSLDIRRKAGLELGKRQVVIRPGEKFEIGDDEFD
                     VDEMIERSHAANRKLATVQLEKKLAQIEVDVAENMTKPQLDVTFSGAVMGDGDNAGTS
                     ISGIGSSYEVSVGLSLSFELSGAARKNRDAALAKKRRLDVDREDIIRQIDTEVVGAVR
                     QVTAARTRVELAEKAIVMAEENAKAEKVSFQAARTTNFQVMQRQTDLIEARLRRGQAV
                     ADYHKAVAQLQFLSGDILPQYRVNVKPRGER"
     gene            8898..10652
                     /locus_tag="VMZ53_32310"
     CDS             8898..10652
                     /locus_tag="VMZ53_32310"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HUS33246.1"
                     /translation="MRIEQKAWGPAADAPTFLPADALAVEGLSVSAAPVTAPRVPKQP
                     KPASRSSSPSSSRSKSDSIITSAPKQPVARSRNPSQSTIKPLPEAPSGPAPRGSSPST
                     PSMQQAAPGAAVPPYVQFEGGASFDPLAIPLPPELAAPVYDWLRRLALQADLSGADKL
                     LREAFADLTSSLSCLIIYAGPDGLHTLGANDEMPKDDSPIVAVARSRRALCTQHNAYL
                     PIATSTETMAVVVLTRNSRQPAYNMVDHVTMAGLARESASIMHHLVVQHLQRRNEAEH
                     DKKGLYRPEALESHRRRGQEGVVAELSPTWVRRTYWVLLICLVAAIVFATWVTVPTYS
                     TGTGIVVYDGEKVTAPAPGTVEKVLVQVNQEVHKGTLLVRLKADKEEADLRQVKTELD
                     KTLQQYLFDRTDEQIRKQLLTVQSQVTRAEDALKQKNIYATADGVVADLRPHEGVPIE
                     FGGSICTIIKPGTTPTILAYMPASDLPRFAVGKQLQVSLTNFEKAPEKVTITSVERSG
                     ISAAEAKRSLGPLLGEGLKLAPETTYVLVKASLPSTFRAKGQTYRYLTGMPAKTEIAV
                     ESKRFVVTLLPSLEKYVP"
     gene            10844..13147
                     /locus_tag="VMZ53_32315"
     CDS             10844..13147
                     /locus_tag="VMZ53_32315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HUS33247.1"
                     /translation="MATDNKAVAESFPALRKLGFIAGKKSVPFTQQLEWTDCGAASLT
                     MVMAYHGKDVKLPEVREAMGIGRDGVSAKSILDTAEKYGLAGRGIKVDIAQIKLLKPA
                     TILHWEFNHFVVFERVVKDGVRIVDPATGPRDVPLAQFAKAFTGVAIELTVTPRFTKK
                     KAEKGRLKRYVEELLSEKGLFSRIIMVSLFLRVIALAMPLVTGMIIDKVVPRSDYHLL
                     YVLLGAIGGMVLFNLISELVRSHLLLHLRIALDTRMTLGFLDHMVSLPFQFFQRRSTG
                     DLMMRVDSNGTVREIVTSKSLSAVIDGLFVLLYAVIIFYVSPKLGFITIAMSFAEALV
                     FIGARPTFQRLLAAHLDKQAKAHSYMVQLLGGMETLKCAGAERLGLEKWSNLYTDELN
                     VTLRRARASAYVDSLRGAVAALGPMLILTIGATAVMRGEMTPGTMLAMNSLATSLFGP
                     LSALVGSALELQLVKGHMDRIDDVLQSSVEQDRDTVQLPHRLRGHVTVKNLSFKYGEQ
                     APLVVQDVSIDIPPGTAVALVGPSGSGKSTLLNLLSGLYKPTKGDISYDNRPLQEMDL
                     RAVRQQIGVVPQHPFIFGGTMRENIALGSPGAALERISGAAKLACLHDDISEMPMNYD
                     TVVSDGGGSLSGGQRQRVAIARSILRNPTLMLLDEATSALDNQTEAKVIANLEKLRCT
                     RITVAHRLSTIRNADLILVMDKGKVVEQGSYAELAQRGGLFSQLLAASQSSPTPGSEL
                     PYEAAFAVRNAPPASSLRNARNSAERR"
     gene            13176..13946
                     /locus_tag="VMZ53_32320"
     CDS             13176..13946
                     /locus_tag="VMZ53_32320"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HUS33248.1"
                     /translation="MAEAPKAPEFVAVITSRKSQLITAPFEGTIMKLQARNGKEVTEG
                     DIVAEIDDTKLKHDLDQAKGQLEQAKGVAAKAGALVANARRRAMTERRLINSGAAAPE
                     AFRNIQAEASSYGAEGASAAGQIKSAKAMIADLEGQLAKAKVPAPFAGTLSVVKVKEG
                     QAARRGEPIARVFDPKELIIAFAVPAAYKKTVKVNTNVEFVTDEGKVVPATIRTAMDD
                     HDPTIEFTTFEAVIDPTYRTDEIRVGDNGHVRIAGAVR"
     gene            13970..15388
                     /locus_tag="VMZ53_32325"
     CDS             13970..15388
                     /locus_tag="VMZ53_32325"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS33249.1"
                     /translation="MEGSDLETPTWVTSYQGLPRTVNVRRCRLEVVAGLDQGKVIELA
                     LPQIQIGRVGADLNLSDGKISGLHCELKLQAEGYRLRDLGSTNGTYVKGVRVVDAFIA
                     PGSIIQIGKSAIKFDPLDDAVALPLWNESRLHNLVGGSAAMRHLFELINRFAQSDATV
                     LIQGETGTGKELIADAIHQCSPRRNEPFIVLDCSAIPEQLFESQLFGHEVGAFTGAVK
                     STPGVFEMAHGGTLFLDEIGELPLDVQAKLLRAVETRKVRRLGGTKVFASDVRLVAAT
                     NRDLAAEVNRGTFRSDLFYRLNVARLQVPALRERREDLPALIEHFLRQLAVSAGVPDP
                     RLPDDFMARAMRHTWPGNVRELRNSVERAMLLPNHPAAAFEQPLKKEGEGFAHIDIDV
                     PFKVAKQKLVDEFDRRYLQQLLEAHDGNISAAARAAGIERMSIYKMIRRLGLDKEADG
                     EGTGTRGRAPTADEDEDTNDDI"
     gene            15401..15850
                     /locus_tag="VMZ53_32330"
     CDS             15401..15850
                     /locus_tag="VMZ53_32330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33250.1"
                     /translation="MGCYRLLFIALVACGSSTPKTGAPLPPWKDMNADQRLEFMKTTV
                     LPAAKAKFQAFDGKKYAKFDCRVCHGKGAEDGSFEMPNPDIKALPGSEEAYMAWVAKD
                     ADAARYTKFMSEDIVPMMAELLGKKPFDPATHTGDFSCPACHKMAGE"
     gene            complement(15851..17563)
                     /locus_tag="VMZ53_32335"
     CDS             complement(15851..17563)
                     /locus_tag="VMZ53_32335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33251.1"
                     /translation="MVRRLAWLTALALVMSCGGDPAPDVENPATVKRKKKKKKKTKVE
                     VADASADEDDKPKKKKKKDDSESESEPKAESAEDKKRKQEEEDAREAEERRERIRKRE
                     QEEAEAAKDSEAEEEAAEEKKRKKEEAAEKKREAAEAKKAAAEEKKRKKEEAAEEARR
                     KKEEEAEAKKEAEAAKKKKKKGREEVEEETSDEEDVAAKESDEDDEKEEKRDEKKASK
                     KDKEKDKKAKKTVAVAKKDTKKQKEEAEEEAEEKKDKEKEKEKEKDDEAEKEVAEADI
                     EIEEDEGGGLPPPDPENESKELDLEGGMPARDLSDDSDKPPPMLAIDRRPLTQEKGRL
                     DVHGGLRIASIKAGMTTATSEGLGLGATYGVTPKVQVGLDYTIGINPGSAKGPFALSG
                     AFQVKHSEKLDVALSAGFAIDFITSTDPMTMMTSTTTVKALQFGVWTRYHLGAKASVF
                     TGLPALPNASGGLSGAAGLPPLPYHLSLGLGQGATIALEVPVGIGYQASPKLYAMAMT
                     SLAHIDMKNSTGAFLFADYIPFSLGGFYAKDAKLDIGAVISDDVKQGLGYLRFDIVAR
                     YYLK"
     gene            complement(17576..18325)
                     /locus_tag="VMZ53_32340"
     CDS             complement(17576..18325)
                     /locus_tag="VMZ53_32340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS33252.1"
                     /translation="MDLGYANKACLVAGASRGIGKAIAHALANEGARVAAVARGKDEL
                     DATVKELGERHIAIVADVSTPEGASAAVDGTLAAFGAIDCVVCVVGKSFARDSAQMDD
                     ADFAKSLDANLWSALRVAQRAAPKMTGGSITFISSIYGREAGGAPGYNIAKAGVIALA
                     KALARDYAPRGIRVNSVAPGSILFPGGGWDRRQKADPAGIADMVARELPFGRFGTPQE
                     VADVVAFVASPRASWIAGACLVVDGAQSRVF"
     gene            complement(18325..19077)
                     /locus_tag="VMZ53_32345"
     CDS             complement(18325..19077)
                     /locus_tag="VMZ53_32345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS33253.1"
                     /translation="MAIVTGGGSGIGLATALELARLGAKVAICGRTAEKLEGAAAELT
                     KVSSQDRVIAATCDIREPQQVEAFVQDVVGDWGQIDILVNNAGGQFPSPAQHITPNGF
                     LAVIKNNLVGTFNMTREVANKSMIARKGGRIVNVIANIYRGFPGMAHTGAARAGVENL
                     TMTLAVEWAQFGILVNAVAPGIIISSGTAQYPPQLLERGVTETPLKRAGTIDEVAASI
                     VFLASPAAQFITGATLRIDGGQALWGHSWEIP"
     gene            19387..21303
                     /locus_tag="VMZ53_32350"
     CDS             19387..21303
                     /locus_tag="VMZ53_32350"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS33254.1"
                     /translation="MLGELLGRGGMAEVFAGHSHGSHGFQKPVAIKRLLPELANDHVF
                     VERLIGEAKLLISLQHGNIVSVLDLARDGEDVFLVMEYVDGPSLRQLIKARGNRGIPM
                     PVASYVVQAAAAGLEFAHARPGGAIVHADISPSNILLTTSGEVRVADFGIARREGGGA
                     GVVEGKWAYMAPEQARGESLTPRSDVFALGVVLYELITGQHPLGKQVSAEERDSGPIR
                     IAPPRVINPKVPAGLDAICMKALSENARDRYARMQHMIDAIIDERFSNGWREGANDLA
                     GLINEVKPGAGTTNAGPRTQVTDRPVTIMTRSLISESALAKRRASSPRLQSQSQSQPQ
                     SQLEYSSPASTSVPSRDFDSIEATHEVSMPTPPRSSAAAIVPPSGARTMLVEMPALPA
                     PMLAPTPEAIAAAAAAQAYARMSSPMLNPELASVHGGHTFTGSAAIDVRDARSNKWTF
                     AVLGIAALLGVIAAVSISTSGGARVEAKPAPAPVEQPVAQEQTAPQAAQTEEVVTPPT
                     VEAPAAIEQPAPAQVPVTETAEHVDTKQETATPTPRDKGRKVRTPVAPKATPTKGDRV
                     LRLESDGGTYARATLNGVSYDVPGGKIKLNAGTYTVKFENRDLGKKFTCPVTVAKDVQ
                     SLKVYMEDGTCDVY"
     gene            21288..22463
                     /locus_tag="VMZ53_32355"
     CDS             21288..22463
                     /locus_tag="VMZ53_32355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33255.1"
                     /translation="MRRLLSSVAIVTLALGAATARAEEMDPNEEPVVVVNAAPKLGNV
                     DQITRLQRVLDSRNMLAKLPDNLEATLDGRNVLIADLDAIREAYGSADYETALKIIET
                     DEQRILGEAINRDPIPALAELSQWRGIISAALGQQDDAVRWFRAMYRFNPAWMIDKKL
                     VTPRVRSMTQKAKREPTETGTLKINCDPEDAMIVIDGADKHQAGEKIKLTTGLHLVMI
                     TANDRKPYAELVEISDGDPYRIDISLDKESNLDRAARIVDASAAAPAGKYRLKRAKGL
                     AKLTGVTRMLFVEDGGDDHITVRLYDLETKKVSKPLDIEGTASSAAVARKIVAALDPE
                     NLVDVNTVVVTQHIVEKQKPTPWYGRWYVWAGVAAIAGGSYLTYDYMTREPTSIRGF"
     gene            22463..23548
                     /locus_tag="VMZ53_32360"
     CDS             22463..23548
                     /locus_tag="VMZ53_32360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33256.1"
                     /translation="MKIILLLLATAACTEPVVEMQLVLPKSESMSAQCITAVEVYANG
                     ATFPANKDDFVESCIEVTPGTTYASVKEAIAGKFTLGMPNTGLSTLEIFGWSGPGGCT
                     FDDNAPPPNLMFFGKGEYIGQDTVDLPIVPNLDCAADPVKIRVVDMMALVGGANCTAA
                     SNIGGGYAALVTLMNSTYKKGVDVYGGTDGGLLMSGVASFNGHTKVGARSCLGVDGGS
                     MTGGSTGCNVGGGTVCAAAGELEHAYISNDIYASALALDQATLAKFPTASLVSVWSNG
                     ATKTPVGGATLEVDPAHAKVFYIDPPAAGQVGLHQRTDNATGASGLALVYADSLVSVT
                     IKAGSTSRTVTLGAPNQEVSANMIVMP"
     gene            23687..24571
                     /locus_tag="VMZ53_32365"
     CDS             23687..24571
                     /locus_tag="VMZ53_32365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33257.1"
                     /translation="MRLLCVAVLVLVGGVADAQPRGRHATTRRVRPSLPAQERVTTVA
                     PVTVEKQLPPGVTLVQGAVLVNMTLEMSVAKTNAGVPASLAPDVSAGVTNDLTLSLVH
                     SGSALTGFRGGAGAGFCFTGEGPGKCKSSYAGGGFEALYGLVRGPIAVGANGGLLVTS
                     VDPRRMDVKVGFKSKATLGRTSLVFNPSVWLALDARNDAMAPHEDQLLLPLGIVQKVT
                     TRFSVGAGTGLRGPLKHLGTQYAIPAGVILQFALDPHVTFGSSLVFGRIVGGSDIMNP
                     EPGLDSRALQLWLSVASR"
     gene            complement(24568..25290)
                     /locus_tag="VMZ53_32370"
     CDS             complement(24568..25290)
                     /locus_tag="VMZ53_32370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33258.1"
                     /translation="MKWSVALGWLVACAPHGGIKVAAPERYEVGEEATISIDAPGADG
                     DSPVEGDIVIVRPDGSVYTQHARLTHPKNRIKIGDSEPTFMQTGRYRLQFQQDANHPL
                     AAPVDISVNIDRLTELLSEMIVDYKAKTRYTKARANGHLHWKQYSGVYEHPWQKDHEI
                     EVVIEEPGEAFKTAWKTYEEQGVLQVMQKNYVRLREGADTTMAAWTSEGRIIVLRATD
                     LAKFDPKFIARFFTRYPSDLKP"
     gene            25419..27965
                     /locus_tag="VMZ53_32375"
     CDS             25419..27965
                     /locus_tag="VMZ53_32375"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HUS33259.1"
                     /translation="MGGHKSPSSKPEPVRGSGSAHVASATAVAAAEPARALVTVRDDK
                     GPLPGATVRFEHEGDVTVVQTGADGTARADALEAGEWDVSASAEGHVPAAAATKTMRA
                     GETTKLEIVLAAGGRMLTGTVTDATGGPIAGARIDAAKLDGSVRPSDAVASTLTGADG
                     KYKLTVAEGQLLVGASEASYAAQSRYVEVGASGATADFQLVPGGVIEGIVRDERSRDP
                     VAGAIVEAERDTPALFGEGASRRATTSKDGRFRLAGLRPGAYSLAAKATNVASRAPTL
                     VGIGVAEQVTDIEILVGRAPAIRGIVVDENGAPAADVEVTAFGDGQSAQATSDAKGAF
                     VLDGIGAGRFTLVGRSEQFLAAGATKIEVSDKDVDDVKVNVQRGLSVNGHVEPRQVCD
                     IEIDTTGRSMGEIRMRIAPITTAADGQFTLGPTQGGGVDINARCASGDQGTKHVEVTA
                     NVTDIVVEVKPGASIAGKVVDGQGKPVVAATVMASPMKETERTTIVNGMLVGGVQSVT
                     NGKGEFELRGLSAGPYRMRVLDRGRPLPMKSDAKVTVAALEKKTGVTLTVERADGVIR
                     GVVTGPDGKPLADAWVSLQQGIDDLIGDPDMGHGESRMVTVESVQVGEGESGDGGGFA
                     PVLTDATGKFEIANLPRVPWTVVAEAQAGKLRGRQVHVTPDANITIQALGITELTGTV
                     KPAPGVFTVELEGPTRAQRSFSSADGSFSFGRVDPGEYAVTVTSSAGNGAGSVIVKPG
                     QPATIDIVLAANATVIGKVADPSGKPLGGVPVAVIPDNNDGKLSVSLSGPPPTTNPDG
                     TFRLEAKAGRSAVIVLVPPRPASKKGLLLEAGKTFDAGTITVDASPPPKP"
     gene            27984..>29836
                     /locus_tag="VMZ53_32380"
     CDS             27984..>29836
                     /locus_tag="VMZ53_32380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33260.1"
                     /translation="MSKQRWKLLLCAIAAAATLVTALFVSAWFHMDVMGSEMEIDLRS
                     ASMCGANGVCVAVPLSKFRGFYSIMGNAAFFAAIPVLILVVVQCASRLVTGAASSAVT
                     KAGYGLGMVALITGFGAGYLFAPQSAGIDDIAVITVTRTWAPLIFVVGNLLALFAMYL
                     ASSEWLDDDVGEYKPIKLDGGRLPVTPLSVNRIHTPQGSQPLQRIPTPQGSQPLQRPG
                     SDGGRSRTPTGQQRAGSEGGRSRTPTGQHAVSESGRSRTPTGQQRAMTESGRSRTPTG
                     QQLSEDVARRLTPSEPIVRIADPSRRLTPSQQTQRVDDPARRFTPSQQIQRVDDPTRR
                     LTPSQQTQRLDDPTRRLTPSGQIPRIDDPSRPKSPSQPPAIARTSSPSGLRSRTPSQQ
                     PLGIVEVDARAKSPSQQPVLARTTSPSGIAIDPASRARTSSSGPIDLAARLSATAPQG
                     VLEPLGGSAGDAPIEFSIRPPLPQPEPVPPDQIPVDPAAGLTIRKRPASVAPPPLAPP
                     LAAAPAIAASPHDEVPLSKAKTVDLPDSAFQPPIAIETPPESGLLIRKKSPTGAPHAP
                     LSASMPAIALDGIEGLAELKDELSKPHAASPASPASPASSIEEALIANADAT"
BASE COUNT         5019 a         9925 c         9584 g         5308 t
ORIGIN      
        1 acgagcgacg gcgacagaaa atcatcgccg cccggctccc agatcgccgg tgcgtcgaca
       61 tcgcgcgcga aatacgagcg gcagcgatcc tcgatcaggt tcgcgagcgc atgttgcttc
      121 acctcctgcg cgtaatcgaa cgcgaagaga aggccgaatg cagtgttggt gtgcgtcccc
      181 gtgcggatcg gataggcctg cttcggcagg aacgcgaggt agtggcctgc gatcacgtcg
      241 gcgagcggcg cgatcgcctc ttcccacggc gtgccacgca ggacgcgcgc gagctcgagc
      301 gtccacgccc agccatacat gcgttcgaac gcgggatgcg ccgcgcaata ctcggcctcg
      361 cgccgcagat tctcgacggt caggtgcgca tcgagcacgc gcttgatctc gctcgcctcc
      421 gccacgtcgg gcgcaagtgc gagcacgcgc gcgagcgtcc agtgaccgtg cacggcggag
      481 tgccaatcga acgcgccgta gaacgcggga tgcagctcgc gatgggaggc gcgctcgcct
      541 tcgcgcgtga ccacgtgatc gagcttgtgc ggcagcgggc gctcgaccgc cgacaacgcg
      601 agtcgcgcga gcgtcgatgc gagctcgcgg ttcacggcac gccgatcact ttgtgcatct
      661 ggacgctgag ccgccagcgc ggccgcgact gcacgagctc gatcgtccgc gcgagatgac
      721 gcggatactc gtcgttcatg cacggctgag cgaagtagtg ctcgcagcga tatttctcgg
      781 cgtagcgatc gagcaccgcg gcatcgacgg tgtcgtcgac gacgaccttc agctcgctca
      841 cgggcacgtc attggcgagc gtgatcgacg tcggatggaa ctgcggtttc ggcgagaccg
      901 tgagccaatc gacggttcca ttcggcacgc gcgtgccgtt gctctccatg tgaatccgaa
      961 agtccgcagc gtggatcgcg tcgctcagcg gctgatccca ctgcagcgtt ggctcgccgc
     1021 ccgtcacgac gaccatgcgt gcgcgatgca cgtcgagctc gaggaggcgc gacacgatct
     1081 cgctagcgtc gtagcgaacc gcgccgtctg gcgcgaagtc ggtatcacac caggggcact
     1141 cgagattgca tgcggcgaac cgcaagaaca cgcacggccg ccccgcgtgc gcgccctcgc
     1201 cttgcagcgt gggcccgaac atctccttca ccaggtagcg cttctcgggc atttcgtcga
     1261 cggcaatgct atagcgcggg cgtgaaccgc tgcgtagtcc tcgccgctct cgtctgtgcg
     1321 gcggcctgcg gcaagcgaca gcaggtggag tcaggcgtgg gctccggcgt tggctcggat
     1381 acctgcgagc agctgccatt cgccgccagc gtgccggttg ccgaagcatc gggcgcggcg
     1441 tggctcgagg tcggcggcaa gcaggcgctt gtcgtcaacg cggacagcgg tcatcgcggc
     1501 gagtacgcca tcatcgatcc cgagacgggc aacgtcatcg agcagggctc gctgccactc
     1561 gggcaagtgc cgaaggacgc cgacgacatc gagggcctcg cgacgcgcgg tggcatgctc
     1621 tatgggctgc agtcgtcggg gtggatgcgc gtgtggaagc gggtcggcag cgggttcgag
     1681 ctcgtcgacg ggccatatcc gatcgcgacg ggcgacaact tctcgtgcgc gatcgacgac
     1741 ggcaactgcg gcaagaacta cgaggggctc gcgatcgcgc cgaagccgaa cgggccatgc
     1801 gccggctttg cctgctcgaa ggaggacggc cacctctact gcctcgtcga gcagcaggac
     1861 ggccggttcg cggtcgatcg gacgaagagc atcgcggtcg agcgcaaggg cgtgctcgcg
     1921 gactgtgcgt tttcgccgct cggcaagctg ttcgtcggga acaacatgtt cggcaattcg
     1981 accgtctacg tcgtcgacgg ctgggccgat cccgcgaagg cccgggtcga ggaagtcggc
     2041 gagctcgggg tcggctttcc cgaagcaatt gccgtcgata gcgccctcat ctataggctg
     2101 tcggacaccg gcggatcccc gagcatgatg gccaaatttc gctgcttggc tccggggcgg
     2161 taaaattaac cagtgcagtg cccgtattgc agcggcgact cgagcgtcac cgagacgcgc
     2221 gtcaccgccg acgggatgcg acgccggcgc gtatgcaccg cgtgcaagcg caggttcacc
     2281 acgtacgaga agctaggcgc gccgctgctc aaggtcgcga agcgcgatgg cagcgtggag
     2341 ccattcgatg gcgagaagct ctttcacgcg ctgcagcgtg tcgcggcgca tcgcgacatc
     2401 agcgacgaag acctgcgacg catcgcgcgc gatatcgagg cgacgctcgt cgattcgagc
     2461 cgcaagtcgg tcacctggaa tgacatcgtc tcgctcgtgc tcgatcgttt gcgcaacgtc
     2521 gatccgatca ccgcgcatcg gtttgccgcg aactacaccg acgagaccgg cgtgttgcga
     2581 ttcgaaaacg acgacaggcc ggctggcgat cctcgccaac tcggcctgcc tttgactgaa
     2641 gaagactagc tcagcgcttc tgcatcatct gctggagcag ctgcatcatc tgaccgtcgc
     2701 tctgctgctt cgtggcagcg agtccttggc cgacggcagt gaccgccttc gcgagctcac
     2761 cgatgccctg aatgagctgc gggtcgatcg cgtcggcacc gcgtgtgtag cggccaccgg
     2821 tcacttcctc gagtgctccc agatcgatcg tcttgaacgg gtcgtcggcc atgacacttc
     2881 tccttaacgg cgaacgttga ctttgacgta cgtgccgcct tgcggaggag gcggagcagc
     2941 cgctgccggt gcgcttccgc cgccgccgcc gccgcccatc atcatcatca tcatcatcat
     3001 ggtggtcatg ctgttgtctt gctgcggctt ggcgacctct ttgatggagt cgccgatctg
     3061 gctgagcatc gtggtgagct tgctgtcgag gtcgctgctc ttgctggtcg ccgcaacgcg
     3121 agccgcgccg ccttgaacct tctcgagggc cgcgtcttcg atcttctgga acttggtctt
     3181 cttcgacatg gggagacttc cttggtggtg gatgtgttgg agggttagag catcggtcgt
     3241 gccagcgagc gatttgtgtg atctcgcgga gctcgcggat cgctcgctgg tcagcgcgcc
     3301 tacgcgagta gacaggcgac cgccacggag aaccaggtca cgcacatcgc cgctgcgatc
     3361 aggtgcgacg agcgatgcgt ggtgcgctgc gtacgaatga acagcgtctt ctctgcagcc
     3421 gctgtcatgt cgatcgtcgg tcgcgaaggg cgacgacgtg gacgtccacc ggcgagcaga
     3481 atccggtcga gcggggtgac gagctgttcg gcgttgatgg tttcgatcgc gtcgaggtcg
     3541 tacatggctg tcagaatcga agtgcagctg acatgccgta caagaagtgc gcgaaacagc
     3601 tcgagctcat gtgtcagtga tcgtcttcgt cggtagacga gttgtctcct gcgaggcgaa
     3661 tgcacgtacc gaacgccaaa acctcgtcga aaccacgccg aaaacacgaa cggcccgaag
     3721 ctcaccagcc tcggaccgtt cgtgaatttc taaggacccg tatcgggcag taggagctgt
     3781 taccagccct tcttgccgca gccgcccttg gcggacactt cgacgtacgt cggttgctgc
     3841 ggagcaggcg ccgcaactgc gccgcctccg ccaccgccca tgccgcccat catcatcatc
     3901 atcatcatca tggtggtcat atcgccgcca ccgctgttgt tgttcttcgc gaggtctttg
     3961 atcgagtcgc cgatcgaggt gagcatgagc atcagctgat cgttggtatc gctgccgcca
     4021 ccggaagcgg cgacgcgagc tgcgccaccg ttgactttgt cgaggtcgtc ggtggtgatc
     4081 gaaacaaacg gatccttgga gttcgacatg gtgattttcc cttggtggag gttggttgcg
     4141 ctgatttctc tatgagcacg gtgtgtgcca cgcggctccc gacgacgtgg acacgtgatc
     4201 accgacggat acacgtgtcg ctgcatgaag tcggcgggtg tccacaccca cgatcgtctc
     4261 cgtcgggaga cagccccatt tcccacctga caaacgccga aaaaacgaac ggcctggatt
     4321 tctccaggcc gccggcgtgc actcggagtg caccgctatt cagaactgta cgccgacggt
     4381 accggtgccc gtgacagctg catcggtgac gtcccacacg ccccacgctt gcgagagctc
     4441 gcccgagccc tcgaagtcga tcttgatgcg ccggtactcg atgccgccct tcacgtagag
     4501 gttgtgcggc agcgggatcg agaagttgcc gtcgaaggcg acgccgttgg ccttacccgc
     4561 gccgtaccag tcttgctccg tcaccatgcc ggcgccgagc aagtacatgt agcgagcgcc
     4621 tgcaccgacc gacgccttct cggtgacctg gaggtcgacg tgtacgccgc cgcccaagta
     4681 cgagaacgac gtatcgggga tcgcaaccga ctgcgggaag tcgcccggca gcgagtgcga
     4741 gacgttgccg aaatccgccg acgcgtcgat cgagacgata tcgatggggt agcggtagtg
     4801 gatgccggtc tgccacgcga cgtggctgat gtcgtagtcg ccgtaggcgg tgtcatccat
     4861 cacggtgaga acgctgccga cggaccgctt cacgtcgaac gagaagccga ggcccgagag
     4921 gcggccgtcg accttctgcg tcggcatggg aaagacttcg atctgcgccg caaacccgcg
     4981 gaggctcgag gccggatacg acggcggcga ctccttcgct tcaggatcgt tcgtgaagtt
     5041 cagaccgcgc gacgagagat aaggacccgc gttgatcgag aaccgcggcg tcggcacggg
     5101 aacgtgcttc gacttaccct cggtcacgat ctcggcttcc ttcgactcgg ggccgaacag
     5161 cgtcacgaga tcattggtgt ctttcgtcgc gatcggatcg gcgacggcaa cctgcttctc
     5221 ttccgccttc gtcttcgacg gtgcggcctc ttcctcgtcg gagaccgctt cgtcggcggg
     5281 ctgctcttcg gcgacgcggt tcttgcgctt cttgtgcttc ttgtggcgcg agcggacggt
     5341 gtcgtcgtcc gttagctcgg cgtcgtcttc gtcgtcttgc tcgtcgcggc tgcgctcgcg
     5401 atccttcgac ggatcctttg cgccgagcag cggcgggcgg tactcgggga ggctctgcgt
     5461 cggctcggcg gtgatgtcgc cgtcgatcca ggcgagcacc tcgtcgagct gcgcagcgag
     5521 cttgtgaccg gcttcggtcg tcacgccgtt gtccttgatg cggacggaca gcgtgtcgat
     5581 ctcgttgccc gtcgaggcat cgcgaaccgc aaccgtcagc acgtggtggc gaccttcggt
     5641 ctgcacccag ccttcgatca ctgcatcgac gccggcttgc ttgctcgctt ggcgccactg
     5701 ctgcggaccg cgacccgacg agcgagcgcg cgccgtgtcc cagttcttcg tcgcgacgat
     5761 gttgtactgg tcaccgagga ccgacatcac cgccgaacga ccgttgtcgg cgagctgacg
     5821 cgggccgtcg aagtcgagga cgacaacgcg gcgcggtttg gcgtcggcat ccgaggcagc
     5881 caacagcgtg gcggcacccg aagcggcgag gagagctagg actttgaagt tgaacatggg
     5941 atggctcctc agctcggcgt tcagcggtag cagttctgct ggttgttcag acagtagtgg
     6001 ttgccgttgc actgattggt cttgttgccg gtcgcggtga atgcgccggt cgcaacgcaa
     6061 cagccgatgt cgtgagcttt cgagacatcg agctgggcgt tgttgttgat cgcgacggtt
     6121 ccgaagatgc gcgtcgcgtg gtcgatggca ccggtgtcgg tcagcttcgc gttgttcgcg
     6181 atcgtcacgt tcgtgtcgac cgtgatggag ctgccgaact gccccagcga gacgagcgcg
     6241 tcgttatcgt cgatcgagaa cgcgccgtgg acgttggtca tcgacgacat gttgctgagc
     6301 gtcgtgagcg ccgggttgcg cgagatgctc acgttgccaa ccgactggag cgacgagagc
     6361 tggatgctcg tcagcaccgt gttgtcgctg atcgtcacgc cgccctcgag acgctgcatc
     6421 gacgtgaagt cgaccgcggt cagcttgagg ttgttggtga tcgacgtcgc gcccgacacg
     6481 atcgaagcgc ggttgaggcc accgaggttc ggcagcagcg ggttgtactc gacgcggatg
     6541 ctcttgaccg tgctcttcgc gacgatgttc gcgatgtcgg tgagcttgtc gttgttgtgg
     6601 atgtagatgt cacccgagac gtcgaccgcg gccttcgtat cgaacttcgc gatgtccgcg
     6661 tcgttgatct cgaggtcggt cacctcgcgc aggtcaccga gcttggcgac gctcgtgatg
     6721 gccgggccgg tgatgacgag cttgccattc agcttccagc acgtcgtcgg gaggttgttg
     6781 aagttcgagg cgcccgagac cgtcaggttc atcgtgacga tcttcaggtc gtcgcaagcg
     6841 ccggtcgccg acgagccatc aacgccggcg tcggggccga agttgtcgtg gtcgcggtcg
     6901 ccaatcggcg ccacacaggc cgagaggaga gaaaccagag cgaggttcgt ggtgcgcatg
     6961 gtgagtccgt aaagcatcgg gcgtgccgtt tcgtgttcgt aaaggatctc ggtggttgag
     7021 cttttcatcg gggtggggga accggtcagc ttgtcaaccg agggatgcac gagtcactta
     7081 gcgctcccca cgcggcttca cgttgacgcg gtactgcggg aggatgtcgc cgctgaggaa
     7141 ctgcagctgc gcgacggcct tgtggtaatc ggcgacggcc tggccgcggc gcaggcgcgc
     7201 ttcgatcaag tccgtctgcc gctgcatgac ctggaagttc gtcgtgcgcg cggcctggaa
     7261 gctcaccttc tcggccttgg cgttctcctc ggccatcacg atggctttct cggcgagctc
     7321 gacgcgcgtg cgtgcggccg tcacttggcg cactgcgccg acgacctcgg tatcgatctg
     7381 gcgaatgatg tcctcgcgat cgacgtcgag gcggcgcttc ttcgcgagtg cggcatcgcg
     7441 gttcttgcgc gctgcgccag agagctcgaa cgagagcgac aggccgacgg agacctcgta
     7501 actgctgccg atcccactga tcgaggtgcc ggcgttgtcg ccatcaccca tcacggcgcc
     7561 ggagaacgtc acgtcgagct gcggcttcgt catgttctcg gccacatcga cctcgatctg
     7621 cgcgagcttc ttctcgagct ggacggtcgc gagcttgcgg ttcgccgcgt ggctgcgctc
     7681 gatcatctcg tcgacgtcga actcgtcatc gccgatctcg aacttctcgc cggggcggat
     7741 gacgacctgg cgcttgccga gctcgaggcc cgctttccga cggatatcca gcgacttctg
     7801 ctcgagcgcg agctgcgcgc ggagcgccgc ttcctggcga atctggatct cgtagacgac
     7861 cgcgttggcc gccgtcgacg gcaccgtgcc cgcgcgaatg aggtcgtgcg tgatgtcttc
     7921 ctgcttcttg gcgagctcga gcgattggtt gcgaacgtcg agctcgtacg aggcgagcgc
     7981 gagatcccag taatcgttga tgaggtcgcg gatgagctgc tcaccggcga gctgcgcctt
     8041 gaccgtcgcc tcggtcgcct ggagatccgc gcgcttctgg ggcgccagcg cgacggccgg
     8101 cccgaagcca cgcacgagcg gctgcttgaa ggtcgccttc aagatcgccg tgttgcgctg
     8161 gagcatgtcg tacgggttgt cgttcgcgtc ggtgccggcc ggagcttgct gctgctcctt
     8221 ggcctgatgg aggcgcgaca cgatgttgta ttcggtgacc ttgcggtcga agcccgcctc
     8281 gagctggatg ttgccgcccg tcggcaggtt gcgtcctagg ccaacaccgc cgtagacctc
     8341 gttattcgcg accaccgaga acggcggcgc ttcgacgtct tcggcgagcg cgctgcgctt
     8401 ggcttccgtc gacgcgctcg cgatccacgc ctggtcgcgg cggctgcctt cagcggcggc
     8461 accggcggcc acgcggtcga tacgggcgcg gacgaggtcg ggcgcgcggg caacggccgc
     8521 ttcgatcaga ttctcgatct tgatcggaag agcggagacc gcttcggttc catccttgtc
     8581 ttcttcatcg cgctgcggct gctcttcttc gttctgcgcg acgcgcgaat gacgcgggcg
     8641 agcagatgcc gaagcggcgg tcgcgagcag gatcgttccg atgacgatgg agcgcatggc
     8701 cagcggctag agcagccgcc atgccgatcg ccaacgtatt gagtcctcgt gtttacgccg
     8761 ctttggatcc ccggggactc gctgcccgcg ggttacaaca tgtcactccc acccccgggg
     8821 aaggagcggt ttttaccggc ggttcgagca gttagccggg aaagcgcgat ggcaaggcgg
     8881 ttgctcatag actcgccatg cgcatcgaac aaaaggcttg gggaccggca gccgacgcgc
     8941 cgacgttcct gcccgccgat gctctcgccg tcgagggcct gagcgtgagc gcagcaccgg
     9001 tgacggcacc gcgtgtaccg aagcagccga agccggcgtc gcgctcgtcg tcgccctcat
     9061 cgtcgcgcag caagtcggac tcgatcatca cgtccgcacc gaagcagccg gtcgcgcgtt
     9121 cgcgtaaccc gtctcagagc acgatcaagc cgctgcccga ggcaccctcc ggcccggcgc
     9181 ctcgtggctc gtccccgtcg acgccctcga tgcagcaggc ggctccgggc gcggccgttc
     9241 cgccctacgt gcagttcgaa ggcggcgcct cgttcgatcc tctcgcgatt ccgctgccgc
     9301 ccgagctcgc tgcaccggtc tacgactggc tgcgtcgtct cgcgctgcaa gcggacctgt
     9361 cgggcgcaga caagctcctg cgtgaagcgt tcgcggatct gacgagctcg ctgtcgtgcc
     9421 tcatcatcta cgccggaccc gacgggcttc acacgctcgg cgcgaacgac gagatgccaa
     9481 aggacgacag cccgatcgtc gcggtcgctc gctcccgtcg cgcactgtgc acgcagcaca
     9541 acgcgtatct gccgatcgca acgtcgacgg agacgatggc tgtcgtggtg ctgacgcgca
     9601 actcgcgcca gcccgcgtac aacatggtcg accacgtcac gatggcgggt ctcgctcgcg
     9661 agagcgcgag catcatgcat cacctcgtcg tgcagcacct gcagcgccgc aacgaggccg
     9721 agcacgacaa gaagggtctc tatcgtcccg aggcgctcga gagccaccgt cgccgcggcc
     9781 aagagggcgt cgttgccgag ctctcgccga cgtgggtgcg ccgcacctac tgggtgctgc
     9841 tgatctgcct cgttgccgcg atcgtgttcg cgacgtgggt gacggttccg acgtactcca
     9901 ccggcaccgg catcgtcgtc tacgacggcg agaaggtcac cgcaccggct cccggcaccg
     9961 tcgagaaagt gctcgtgcag gtgaaccagg aagtgcacaa gggcaccctg ctcgtccgcc
    10021 tcaaggccga taaagaagag gccgacctcc gccaggtgaa gaccgagctc gacaagacgc
    10081 tccagcagta cctgttcgac cgcaccgacg agcagattcg caagcaactg ctcacggttc
    10141 agtcgcaagt gacgcgcgcc gaggatgcac tcaagcagaa gaacatctac gcgacggccg
    10201 acggcgtcgt cgccgacctt cgcccgcacg aaggcgtgcc catcgagttc ggcggatcga
    10261 tctgcacgat catcaagccg ggcacgacgc cgacgatcct cgcgtatatg ccggcgagcg
    10321 acctgccccg cttcgcggtc ggcaagcagc tgcaggtctc gctcaccaac ttcgagaagg
    10381 ctcccgagaa ggtcacgatc acgtcggtcg agaggtccgg catctcggcg gcggaagcga
    10441 agcgctcgct cggcccgctc cttggcgaag gtctcaagct cgcgcccgag acgacgtatg
    10501 tgctcgtgaa ggcctcgctc ccgtcgacgt tccgcgcgaa gggccagacg taccgctacc
    10561 tgaccggtat gcccgcgaag acggagatcg ccgtcgagag caagcgcttc gtcgtcacgc
    10621 tcctgccgtc gctcgagaag tacgtcccct gacgcgcgca tccgcgtcgc tcgagccccg
    10681 cttcaccgcg gggtttttcg ttttcggcgt tggagttacg cgagctggga gtcatcggtg
    10741 acgggtgctt ccccaggttg acaacgtgtc gccgacaaaa ctcgcaacca tcgaaatctg
    10801 ttgtcaccag aggtcggcac accagctgct ttaagagctc accatggcca ccgacaacaa
    10861 agcagtcgca gagagctttc cggctcttcg caagctcggc ttcatcgcgg gcaagaagtc
    10921 ggtgcccttc acccagcagc tcgagtggac cgattgcggc gccgcttcgc tgacgatggt
    10981 gatggcgtac cacggcaagg acgtgaagct gcccgaggtt cgcgaggcga tgggcatcgg
    11041 tcgcgacggc gtcagcgcga agtcgatcct cgacacggcc gagaagtacg gcctcgccgg
    11101 ccgcggcatc aaggtcgaca tcgcgcagat caagctgctc aagcccgcca cgatcctcca
    11161 ctgggagttc aaccactttg tcgtgttcga gcgcgtcgtc aaagacggcg ttcgcatcgt
    11221 cgacccggcg acgggcccgc gcgatgttcc gctcgcgcag ttcgcgaagg cgttcaccgg
    11281 cgtcgcgatc gagctcacgg tgacgccgcg cttcacgaag aagaaggccg agaagggccg
    11341 cttgaagcgc tacgtcgagg agctcctctc ggagaagggc ctcttctcgc gcatcatcat
    11401 ggtgtcgctg ttcctgcgcg tgatcgcgct cgcgatgccg ctcgtcaccg gcatgatcat
    11461 cgacaaggtc gttccccgca gcgactacca cctgctgtac gtgctgctcg gtgcgatcgg
    11521 cggcatggtg ctcttcaacc tgatctcgga gctcgtgcgc tcgcacctgc tgctccacct
    11581 ccgcatcgcg ctcgacacgc gcatgacgct cggcttcctc gaccacatgg tctcgctgcc
    11641 gttccagttc ttccagcgcc ggtcgacggg cgatctcatg atgcgcgtcg actcgaacgg
    11701 tacggtccgc gagatcgtga cgagcaagtc gctctcggcg gtcatcgacg gcctgttcgt
    11761 tctgctctac gccgtcatca tcttctacgt cagcccgaag ctcggcttca tcacgatcgc
    11821 gatgtcgttc gccgaagcgc tcgtgttcat cggcgcccgc ccgacgttcc agcgtctgct
    11881 cgcggcgcac ctcgacaagc aggcgaaggc gcacagctac atggtccagc tgctcggcgg
    11941 catggagacg ctcaagtgcg caggcgccga gcgcctcggc ctcgagaagt ggagcaacct
    12001 ctacaccgac gagctcaacg tcacgctccg ccgcgctcgt gcttcggcgt acgtcgacag
    12061 cctccgcggt gcggtcgctg ccctcggccc gatgctcatc ctgacgatcg gcgcgacggc
    12121 ggtcatgcgc ggcgagatga cgcccggcac gatgctcgcg atgaactcgc tcgcgacgtc
    12181 gctgttcggc ccgctgtcgg cgctcgttgg ctcggcgctc gagctgcagc tcgtcaaagg
    12241 ccacatggac cgcatcgatg acgtgctgca gtcctcggtc gagcaggatc gcgacaccgt
    12301 ccagctgccg caccgcctcc gcggtcacgt cacggtgaag aacctctcgt tcaagtacgg
    12361 cgagcaagcg ccgctcgttg tccaagacgt ctcgatcgac atcccgcccg gcacggccgt
    12421 cgcgctcgtc ggtccctcgg gctcgggcaa gtcgacgctg ctgaacctcc tctcgggtct
    12481 ctacaagccg acgaagggcg acatctcgta cgacaaccgc ccgctccaag agatggacct
    12541 ccgcgcggtg cgccagcaga tcggcgtcgt cccgcagcac ccgttcatct tcggcggcac
    12601 gatgcgcgag aacatcgcgc tgggctcgcc gggcgctgcg ctcgagcgca tctctggcgc
    12661 cgcgaagctc gcgtgcctgc acgacgacat ctccgagatg ccgatgaact acgacaccgt
    12721 tgtgtcggat ggcggcggct cgctctcggg tggtcagcgc cagcgcgtcg cgattgctcg
    12781 ctccatcctg cgcaacccga cgctgatgct cctcgacgaa gcgacgtcgg cgctcgacaa
    12841 ccagaccgaa gcgaaggtca tcgcaaacct cgagaagctt cgctgcacgc gcatcaccgt
    12901 cgcccaccgt ctgtcgacca tccgcaacgc cgacctcatc ctcgtcatgg acaagggcaa
    12961 ggtcgtcgag cagggctcgt atgccgagct cgcgcagcgc ggcggtctct tctcgcaact
    13021 cctcgctgca tcgcagtcgt ctcccacccc tggatcggag ctcccctatg aagccgcatt
    13081 cgccgttcgt aacgctcctc ctgctagcag cctgcggaac gcacgaaata gcgccgagcg
    13141 ccgctgaccc gacgaaggtc gagctcgctg caccggtggc agaagcaccc aaggcacccg
    13201 agttcgtcgc cgtgatcacc tctcgcaagt ctcagctcat caccgccccg ttcgagggca
    13261 ccatcatgaa gctccaagca cgcaacggta aggaagtcac ggaaggcgac atcgtcgccg
    13321 agatcgatga caccaagctg aagcacgacc tcgaccaggc gaagggccag ctcgagcaag
    13381 cgaagggcgt cgcggcgaag gccggtgcac tcgtcgcgaa tgcgaggcgc cgcgcgatga
    13441 cggagcgccg cctgatcaac agtggagcgg cagcgccgga agcgttccgc aacatccaag
    13501 cggaagcgag ctcgtacggc gccgagggcg catcggccgc cggtcagatc aagagcgcga
    13561 aggcgatgat cgcggacctc gaaggccagc tcgcgaaggc gaaggtcccc gccccgttcg
    13621 ccggcacgct gtccgtcgtc aaggtcaaag agggccaggc ggcgcgccgc ggcgagccca
    13681 tcgcccgcgt gttcgatccc aaagagctga tcatcgcgtt cgcggtgccg gctgcctaca
    13741 agaagaccgt caaggtcaac accaacgtcg agttcgttac cgacgaaggc aaggttgtcc
    13801 ccgcaacgat tcgtacggcg atggacgacc acgacccgac catcgagttc accacgttcg
    13861 aggccgttat cgaccccacc taccgcaccg acgagatccg cgttggtgat aacggacatg
    13921 tccggatcgc aggagcagtg cgatgagcgt gctacgttct caagaagccg tggaaggttc
    13981 cgacctcgaa accccgacct gggtgacgtc ttatcaaggc ctgccgcgta cggtgaacgt
    14041 tcgccgttgc cgcctcgaag tcgtcgccgg acttgatcaa ggcaaagtga tcgagctcgc
    14101 gctgccgcag atccagatcg gccgcgttgg cgccgacctg aatctcagcg acggcaagat
    14161 ctccggtctg cactgcgagc tgaagctcca ggccgaaggc taccgcctgc gcgacctggg
    14221 ctcgacgaac ggcacgtacg tcaaaggcgt gcgcgtggtc gatgccttca tcgctccggg
    14281 ctcgatcatc cagatcggca agtcggccat caagttcgac ccgctggacg acgctgttgc
    14341 gctgccgctc tggaacgagt cgcgcctgca caacctcgtc ggtgggtccg ccgcgatgcg
    14401 ccatctgttc gagctcatca accgcttcgc tcagtcggac gcgaccgtcc tcatccaggg
    14461 cgagaccggc accggcaaag agctcatcgc cgacgcgatt catcagtgct cgccgcgccg
    14521 taacgagccg ttcatcgtgc tcgactgctc cgcgattccc gagcagctgt tcgagtcgca
    14581 gctcttcggc catgaagtgg gcgcattcac cggcgcggtg aagtcgaccc cgggcgtctt
    14641 cgagatggca cacggcggca cgctgttcct cgacgaaatc ggcgagctgc ccctcgacgt
    14701 gcaggcgaag ctgctccgcg cggtcgagac tcgcaaggtc cgccgcctcg gtggtacgaa
    14761 ggtcttcgcg tcggacgttc gcctcgtcgc cgcgacaaac cgcgacctcg ccgctgaagt
    14821 gaaccgcggc acgttccgct cggatctctt ctaccgcctg aacgtcgcgc gcctccaggt
    14881 gccggcactc cgcgagcgcc gcgaggatct ccccgcgctc atcgagcact tcctccgcca
    14941 gctggccgtc agcgcgggcg tgcccgaccc tcgcctgccc gatgacttca tggcgcgcgc
    15001 gatgcgccac acgtggcctg gcaacgttcg cgagctccgc aactccgtcg agcgcgcgat
    15061 gctgctgccg aatcacccgg ccgccgcgtt cgagcagccg ctcaagaaag agggcgaagg
    15121 cttcgctcac atcgacatcg atgtgccgtt caaggtggcc aagcagaagc tcgtcgacga
    15181 attcgatcgc cggtatctgc aacagctgct cgaagcgcac gacggcaaca tctccgctgc
    15241 cgcacgcgcc gccggtatcg agcgcatgtc gatctacaag atgattcgcc gcctcggtct
    15301 cgacaaagaa gccgacggtg aaggcaccgg cactcgcggc cgcgcgccga cggcggacga
    15361 agacgaagac acgaacgacg acatctgata gacacccttc gtgggttgct atcggttgct
    15421 gttcatcgcg ctcgtcgctt gtggttcgtc aacgccgaag acaggcgcgc cgctgccgcc
    15481 gtggaaggac atgaacgcag accagcgtct cgagttcatg aagaccacgg tgctgccagc
    15541 tgcgaaggcg aagttccagg cgttcgacgg caagaagtac gcgaagttcg attgccgcgt
    15601 ctgccacggc aaaggagcgg aggacggctc gttcgagatg ccgaacccgg acatcaaggc
    15661 gctgccggga agcgaagagg cgtacatggc gtgggtcgcc aaagacgccg acgccgcgcg
    15721 ctacacgaag ttcatgagcg aggacatcgt gccgatgatg gcggagctgc tcggcaagaa
    15781 gccgttcgat ccggcaacgc acaccggcga cttctcgtgc cccgcctgcc acaagatggc
    15841 cggcgagtag ctacttcaag tagtagcgag cgacgatgtc gaagcgcagg tagccgaggc
    15901 cctgcttgac gtcgtcggag atcacggcgc cgatgtcgag cttcgcgtct ttcgcgtaga
    15961 agccgccgag cgagaacggg atgtagtcgg cgaacagaaa cgcgccggtc gagttcttca
    16021 tgtcgatgtg cgcgaggctc gtcatcgcca tcgcatagag cttcgggctc gcctggtagc
    16081 cgatgccgac gggaacctcg agcgcgatcg tcgcgccttg gccaaggccg agcgagaggt
    16141 gatacggaag cggcggcagc cccgcggcgc cggaaagacc gccgctcgcg ttcggcagcg
    16201 cgggaagccc ggtgaacacg gacgccttgg cgccgaggtg atagcgcgtc cacacgccga
    16261 actgcagcgc cttcacggtg gtcgtcgacg tcatcatcgt catcggatcc gtcgacgtga
    16321 tgaagtcgat cgcgaatccc gcggacagcg cgacgtcgag cttctcgctg tgcttcacct
    16381 ggaacgcgcc cgagagcgcg aacggtccct tcgcagagcc ggggttgatg ccgatcgtgt
    16441 agtcgaggcc gacttggact ttcggcgtca cgccgtatgt cgcgccgagc ccgaggccct
    16501 ctgacgtcgc ggtcgtcatg ccggccttga tcgaggcgat ccgcagacca ccgtggacgt
    16561 ccagcctgcc cttctcttgc gtgagcgggc gacgatcgat cgcgagcatc ggcggcggct
    16621 tgtcgctgtc gtcggagaga tcacgcgccg gcatgccgcc ctcgaggtcg agctccttgc
    16681 tctcgttctc gggatcggga ggcggaaggc caccgccttc gtcttcctcg atctcgatat
    16741 cggcttcggc gacctctttc tctgcctcgt cgtccttctc tttctctttc tctttctctt
    16801 tgtctttctt ctcttcggcc tcttcctcgg cctcttcctt ctgcttcttc gtgtccttct
    16861 tcgcgaccgc gacggtcttc ttcgccttct tgtccttctc tttgtccttc ttcgaggctt
    16921 ttttctcgtc gcgcttctct tccttctcgt cgtcctcgtc ggactctttg gccgcgacat
    16981 cttcttcgtc ggaggtctct tcctcgactt cctcgcggcc cttcttcttc ttcttcttcg
    17041 ctgcctcggc ttccttcttc gcctcggcct cttcttcctt cttgcgccgc gcttcttcgg
    17101 cggcctcttc cttcttgcgc ttcttctctt cggccgccgc cttcttggct tcggccgctt
    17161 cgcgcttctt ctccgccgcc tcttccttct tgcgcttctt ctcttcggcg gcttcttcct
    17221 cggcttcgct gtccttcgcg gcctcggcct cttcttgctc gcgcttgcga atgcgctcgc
    17281 gacgctcttc cgcctcgcgc gcgtcctctt cctcttgctt gcgcttcttg tcctcggcgc
    17341 tctcggcctt gggctcggac tccgactcgg agtcgtcctt cttcttcttc ttcttcggct
    17401 tgtcgtcttc gtccgccgaa gcgtcggcga cctcgacctt cgtcttcttt ttcttcttct
    17461 tcttgcgctt caccgtcgcc ggattctcga cgtcgggcgc aggatcgccg ccgcacgaca
    17521 tgacgagggc aagagccgtc agccaggcaa ggcgccgcac cacggcttta ttctactaga
    17581 agactcgcga ctgtgccccg tcgactacaa gacacgcccc tgcgatccag ctcgcgcgcg
    17641 gcgacgcgac aaacgcaaca acgtccgcca cttcctgggg cgtgccgaat cggccaaacg
    17701 gcagctcgcg tgcgaccatg tcggcgatgc ctgccgggtc cgccttctgg cggcgatccc
    17761 agccgccacc gggaaacagg atcgagccgg gcgcgacgga gttcacgcgg atgcctcgcg
    17821 gcgcgtaatc acgagcgagc gcctttgcga gcgcgatgac gcccgccttc gcgatgttgt
    17881 agccaggcgc gccgcccgct tcgcgaccat agatcgacga gatgaacgtg atcgagccac
    17941 cggtcatctt cggcgcggca cgctgcgcga cgcgcagggc cgaccacaga tttgcgtcga
    18001 gcgatttcgc gaagtccgca tcgtccatct gcgccgagtc gcgcgcgaat gacttgccga
    18061 cgacgcacac gacgcaatcg atcgcaccga acgctgcgag cgttccgtcg acggcagccg
    18121 atgcgccttc gggcgtcgag acatccgcga cgatcgcgat gtgccgctcg ccgagctctt
    18181 tcaccgttgc atctagctcg tctttgcccc gcgcgaccgc ggcgacacgc gcgccttcgt
    18241 tcgccagcgc atgcgcgatc gctttgccga taccgcggct cgcaccggcg acgagacacg
    18301 ccttgttcgc gtaaccgaga tccattacgg gatctcccag ctgtggcccc acagtgcttg
    18361 gccaccgtcg atgcgcagcg tcgcgcccgt gatgaactgc gccgcgggcg acgcgaggaa
    18421 gacgatcgat gcagcgacct cgtcgatcgt gcccgcgcgc ttgagcggcg tctcggtgac
    18481 tccgcgctcg agcagctgcg gcggatactg ggccgttccc gacgagatga tgatgccggg
    18541 cgcgactgcg ttcacgagga tcccgaactg cgcccactcg acggcgagcg tcatcgtcag
    18601 gttctcgacg ccggcgcgcg ctgcacccgt atgcgccatg ccggggaagc ctctatagat
    18661 gtttgcgatc acgttgacga tgcgaccgcc ctttctcgcg atcatcgatt tgttcgccac
    18721 ctcgcgcgtc atgttgaacg tgccgacgag gttgttcttg atgacggcga gaaatccatt
    18781 cggcgtgatg tgctgcgcgg gcgacgggaa ctgaccgccg gcgttgttga cgaggatgtc
    18841 gatctgcccc cagtcgccga ccacgtcttg tacgaacgct tcgacctgtt gcggctcgcg
    18901 gatatcgcag gtcgcggcga tcacgcggtc ctggctcgag actttcgtga gctcggccgc
    18961 ggcaccttcg agcttctccg cggtgcgtcc gcagatggcg actttggcgc cgagccgggc
    19021 gagctccaac gccgtcgcga ggccgatacc actcccgccg ccagtcacga tcgccacctg
    19081 atttgcgaag atgttagggg cgaacacccg ctgtgattcc gccgacggct tgtgcatttc
    19141 gctgcgcaag ataaacgaag tgtgggggtt acagcagggg gtgggttccc caggttgggg
    19201 gtggctactc caggatccac cctgctccac tgactgggac tgtccgcact gacgccgaag
    19261 tctatgaatg ttcgatctgt tagcccgggc acgctggatg cacatgtcca gctcgcagtg
    19321 gccgggacat ccacagccat aggggagctc cgctctccat ctgagacgcg tcgccaaggg
    19381 cggtacgtgc tcggcgagct actcggaaga ggcggcatgg cggaagtgtt cgccggtcac
    19441 tcgcacggct cgcacggttt ccaaaagccg gtcgccatca agcgcctgct tcccgagctc
    19501 gcgaatgacc acgtgttcgt cgagcgcctg atcggcgagg cgaagctgct catctcgctg
    19561 cagcacggca acatcgtgtc cgttctcgat ctcgctcgcg atggcgaaga cgtcttcctc
    19621 gtcatggagt acgtcgacgg cccgagcttg cgccagctca tcaaagctcg cggcaaccgc
    19681 ggcattccaa tgccggtcgc gagctacgtc gtgcaagcgg ccgccgcggg cctcgagttc
    19741 gcgcatgcgc gcccgggtgg cgccatcgtt cacgccgaca tcagcccgag caacatcttg
    19801 ctcacgacgt cgggcgaagt tcgcgtcgcc gacttcggca ttgcgcgccg tgaaggcggt
    19861 ggcgctggcg tggtcgaagg caagtgggcg tacatggcgc ccgagcaagc gcgcggtgaa
    19921 tcactcacgc cacgcagcga cgtgttcgcg ctcggcgtcg tgctctacga gctcatcacc
    19981 ggccagcatc cgctcggcaa gcaagtcagc gcggaagagc gcgactcggg accgatccgt
    20041 atcgcaccgc cgcgcgtcat caacccgaag gttcccgcag gcctcgatgc gatctgcatg
    20101 aaggcgctgt cagagaatgc tcgcgatcgc tacgcgcgca tgcagcacat gatcgacgcg
    20161 atcatcgacg agcgcttcag caacggctgg cgcgagggtg cgaacgatct cgcgggtctc
    20221 atcaacgagg taaagccggg cgctgggacg accaacgccg gtccgcgcac gcaagtcacg
    20281 gatcgccccg tcacgatcat gacgcgctcg ctgatcagcg agtccgcact cgcgaagcgc
    20341 cgcgcgtcgt cgccgcgcct gcaatcgcaa tcgcaatcgc agccgcagtc gcagctcgag
    20401 tactcgagcc cggcgtcgac gtctgtaccg tcgcgcgact tcgattcgat cgaagcgacg
    20461 cacgaagtct cgatgccgac gccgccgcgc agcagcgccg ccgccatcgt tccgccgtcg
    20521 ggcgcacgca cgatgctcgt cgagatgccg gcacttcccg cgccgatgct cgcgccgacg
    20581 cccgaagcaa tcgcagcggc cgctgccgcg caggcctacg cgcgcatgtc gtcgccgatg
    20641 ctgaaccctg agctcgcgtc ggtgcacggc ggccacacgt tcacgggctc ggccgcgatc
    20701 gacgtgcgcg acgcgcgcag caacaagtgg acgtttgcgg tcctcggtat cgctgcgctc
    20761 ctcggcgtga tcgccgcggt gtcgatcagc acctccggtg gtgcgcgagt ggaagcgaag
    20821 ccggcgccgg ctcccgtcga gcagccggtc gcgcaggagc aaacggcacc gcaggcagcg
    20881 cagaccgagg aagtcgttac gccgccaacg gttgaagcgc ccgctgcgat cgagcaaccc
    20941 gcgcccgcgc aggtccccgt cacggagacc gcggagcacg tcgatacgaa gcaagagacc
    21001 gcgacgccca ccccgcgcga caaaggccgc aaggtccgca cgcccgtcgc gccgaaggcg
    21061 acgccgacga agggtgaccg cgtgcttcgc ctcgagagtg acggcggtac atacgcgcgc
    21121 gcgacgctca acggcgtcag ctacgacgtc cccggcggca agatcaagct caacgcgggc
    21181 acgtacacgg tgaagttcga gaaccgtgac ctcggcaaga agttcacgtg ccccgtgacc
    21241 gtggccaagg acgtccagtc tctcaaggtc tacatggagg acggcacatg cgacgtctac
    21301 tgagcagcgt cgcgatcgtc acgcttgcac tcggtgcggc gactgcgcgt gcagaagaga
    21361 tggatccgaa tgaggagccg gtcgtcgtcg tgaacgcggc gcctaagctc ggcaacgtcg
    21421 accaaatcac gcggctgcag cgcgttctcg attcgcgcaa catgctcgcg aagctccccg
    21481 ataacctcga agcgacgctc gacggccgca acgtcctgat cgcggacctc gacgcgatcc
    21541 gcgaagcata cggctcggcg gactacgaga ccgcactcaa gatcatcgag accgacgagc
    21601 aacgcatcct cggtgaagcg atcaaccgcg atccgattcc tgctcttgcc gagctcagcc
    21661 aatggcgcgg catcatctct gcggcactcg gtcagcagga cgacgccgtc cgctggttcc
    21721 gtgcgatgta tcgcttcaac ccggcgtgga tgatcgacaa gaagctcgtc acgccgcgcg
    21781 ttcgctcgat gacgcagaag gcaaagcgcg agcccaccga gaccggcacg ctgaagatca
    21841 actgcgatcc cgaagacgcg atgatcgtga tcgacggcgc ggacaagcat caggcgggcg
    21901 agaagatcaa gctgaccacc ggcctgcacc tcgtgatgat tacggccaac gaccgcaagc
    21961 cgtacgccga gctcgtcgag atctccgacg gcgacccgta ccgcatcgac atcagcctcg
    22021 acaaggaatc gaacctcgac cgcgctgctc gcatcgtcga cgccagcgcc gcagcgcccg
    22081 ccggcaagta ccgcctgaag cgcgcgaagg gtctcgcgaa gctcacgggt gtcacgcgca
    22141 tgctgttcgt cgaagacggc ggcgatgacc acatcacggt tcgcctttac gacctcgaga
    22201 caaagaaggt ctcgaagccg ctcgacatcg agggcacggc gtcgtcggca gcagtggctc
    22261 gcaagatcgt cgccgcgctc gatcccgaga acctcgtcga cgtgaacacc gtcgtcgtga
    22321 ctcagcacat cgtcgagaag caaaagccga cgccgtggta cggccgttgg tacgtgtggg
    22381 caggcgtcgc cgcgatcgca ggcggctcgt acttgaccta cgactacatg actcgcgaac
    22441 cgacttcgat cagaggcttc tgatgaagat catcctcctc ctccttgcta cggctgcgtg
    22501 cacagagccc gtcgtcgaga tgcagctcgt gctgccgaag tccgagagca tgagcgcgca
    22561 gtgcattact gcggtcgagg tctacgcgaa cggcgccacg ttccccgcaa acaaagacga
    22621 cttcgtcgaa tcgtgcatcg aggtcacgcc cggcacgacg tacgcgtcgg tgaaagaagc
    22681 gatcgccggc aagttcacgc tcggcatgcc gaacacggga ctctcgaccc tcgagatctt
    22741 cggctggtcc gggccgggcg gctgcacgtt cgatgacaac gcgcctccgc caaatctcat
    22801 gttcttcggc aagggcgaat acatcggtca agacactgtc gatcttccga tcgttccgaa
    22861 cctcgactgc gcggccgatc cggtgaagat tcgcgtcgtc gacatgatgg cgctcgtcgg
    22921 tggcgccaac tgcacggccg catcgaacat cggtggtggc tatgcggcgc tcgtcacgct
    22981 gatgaacagc acgtacaaga agggcgtcga cgtctacggc ggtaccgacg ggggactgct
    23041 catgagcggc gtcgcgtcgt tcaacggcca cacgaaggtc ggcgcgcgaa gctgcctcgg
    23101 tgtcgacggc ggcagcatga ccggcggcag caccggttgt aacgtcggcg gcgggaccgt
    23161 atgtgcagcg gccggcgagc tcgagcacgc gtacatctcg aacgacatct acgcatcggc
    23221 gctcgcgcta gatcaggcga cgctcgcgaa gttcccgacg gcctcactcg tctcggtgtg
    23281 gagcaacggc gccacgaaga cgcccgtcgg cggcgcgacc ctcgaagtcg atcccgcgca
    23341 cgcgaaagtg ttctacatcg atccaccggc agccggccaa gtcggtcttc accagcggac
    23401 cgacaacgcg accggcgcgt cgggtctcgc tctcgtgtat gcggactcgc tcgtctccgt
    23461 cacgatcaaa gccggaagca catcgcgcac agtcacgctt ggtgctccaa accaggaggt
    23521 ctccgccaac atgatcgtca tgccgtagtc aggaccgtct cattgcaccg caaacgaggt
    23581 gttccggtcg tccgccgttg aatacccgaa cgcgtgagcg tgagcttccg tacgacggct
    23641 agatgtaggg ttcaaaccgc cccatgtgat gtgcgagcgt tgtcgcgtgc ggttgctgtg
    23701 cgtcgcggtg ttggtgttgg tcggcggggt cgcggatgcg cagccgcgcg gtcgacatgc
    23761 gacgacgcgt cgcgtgcggc catctcttcc cgcgcaggag cgcgtgacga cggtcgcgcc
    23821 ggtgaccgta gagaagcagc tgccgccagg tgtgacgctc gtgcagggcg ccgtgctcgt
    23881 caacatgacg ctcgagatga gtgtcgcgaa gacgaacgcc ggcgtgcccg catcgctcgc
    23941 gcccgatgtg tccgcaggag tcacgaacga tttgacactc tcgctcgttc actcgggctc
    24001 tgcgctcacg ggctttcgcg gcggcgccgg tgcgggtttc tgtttcaccg gcgagggtcc
    24061 gggcaagtgc aagagctcgt acgcgggcgg cggcttcgaa gcgctctacg gcctcgtgcg
    24121 cggcccgatc gccgtcggcg cgaatggtgg cctgctcgtc acgtcggtcg atccgcggcg
    24181 catggatgtc aaggtcggct tcaagtcgaa ggcgacgctc ggccggacgt cgctcgtgtt
    24241 caacccgagc gtgtggctcg cactcgacgc gcggaacgac gcgatggccc cacacgaaga
    24301 tcagctgctg ttgccgctcg gtatcgtgca gaaggtgacg acgcgattct ccgtcggtgc
    24361 gggcaccggc cttcgcgggc cgctgaagca cctcggcacg cagtacgcga ttccggccgg
    24421 cgtcatcctg cagttcgcgc tcgatccgca cgtgacgttc ggctcgtcac tcgtgttcgg
    24481 ccgcatcgtc ggcggcagcg acatcatgaa tccagagccg gggctcgact cgcgtgcgct
    24541 gcagctgtgg ctcagcgtcg cgtcgcgcta gggcttcagg tcgctcggat atcgcgtgaa
    24601 gaatcgcgcg atgaatttcg gatcgaactt cgcgaggtcg gtcgcgcgca gcacgatgat
    24661 gcgaccttcg ctcgtccacg cggccatcgt ggtgtcagcg ccttcgcgaa gccgcacgta
    24721 gttcttctgc atcacctgca gcacgccctg ctcttcgtag gtcttccacg ccgtcttgaa
    24781 tgcttcgccc ggctcttcga taacgacctc gatctcgtga tctttttgcc acgggtgctc
    24841 gtagacgccg gagtactgct tccaatgaag gtgaccgttg gcgcgcgcct tcgtgtagcg
    24901 agtcttcgcc ttgtagtcga cgatcatctc ggagagcagc tcggtgaggc gatcgatgtt
    24961 gaccgagata tcgacgggcg cagcgagcgg atggttcgcg tcctgttgga actgcagccg
    25021 atagcggccg gtctgcatga acgtcggctc gctgtcgccg atcttgatgc gattcttggg
    25081 atgcgtgagt cgcgcgtgct gcgtatagac gctgccgtcg ggacggacga tgacgatgtc
    25141 accttcgacg ggcgaatctc cgtcggcgcc gggcgcgtcg atcgagatgg tggcttcttc
    25201 gccgacctcg taacgctcgg gcgctgcgac cttgatgcct ccgtgcgggg cacacgccac
    25261 gagccagccg agcgctacag accacttcat acgatcctca ccattgtagg gaattctgtg
    25321 gtcgtcgtgc tggttgaggt gcgcgcatga ggaagagcgt cgtcgctttg cttggagtct
    25381 gcgttgtggt tgcggtcgcg atgatcgttt ggttgaagat ggggggccac aaatcgccct
    25441 cgtcgaagcc ggagcctgtg cgcggttcag ggtcagcaca cgtcgcgtcg gcgacggcgg
    25501 tcgcagccgc ggagccagca cgggcgctcg tcaccgtgcg cgatgataaa gggccgctcc
    25561 cgggtgccac cgtacggttc gagcacgaag gcgatgtgac ggtcgtgcaa accggcgccg
    25621 atggcaccgc gcgcgccgat gcgctcgaag ccggagagtg ggacgtgagc gcgtccgcag
    25681 agggtcacgt gccagctgca gccgccacca agacgatgcg cgccggagag acgacgaagc
    25741 tcgagatcgt gcttgctgcg ggtggccgca tgttgaccgg cacggtgacc gacgcgaccg
    25801 gcgggccgat cgcaggtgcg cgcatcgatg cagcgaagct cgacggctcg gtgcgtccat
    25861 ccgatgcagt cgcaagcacg ctcactggcg ctgatggcaa gtacaagctg accgtcgctg
    25921 aagggcagct gctcgtcggt gcgagcgagg cgtcgtatgc ggcgcagtcg cgctacgtcg
    25981 aagttggtgc gtcgggtgcg acggcggatt tccagctcgt tcccggcggc gtgatcgagg
    26041 gaatcgtgcg cgacgaacgt tcgcgagatc ctgttgccgg cgcgatcgtc gaggcggagc
    26101 gagatacacc cgcgctgttc ggcgaaggcg caagccgccg cgcgacgacg agcaaggacg
    26161 gtcgctttcg cctcgcgggt cttcggcccg gtgcttactc gctcgcggcc aaagcgacga
    26221 acgtcgcctc gcgggccccg acgctcgtcg gcatcggcgt cgcggaacaa gtcaccgaca
    26281 tcgagatcct cgtcggtcgc gcgccggcga ttcgcgggat cgtcgtcgac gagaatggtg
    26341 cgccggccgc tgacgtcgag gtcaccgcgt tcggcgacgg tcaatctgca caggcgacct
    26401 ccgatgcgaa gggcgcgttc gtcctcgatg gtatcggcgc aggacgcttc acgctcgtcg
    26461 gccgcagcga gcagttcctg gcggcgggcg cgacgaagat cgaggtgagc gacaaagatg
    26521 tcgacgacgt caaagtgaac gtccagcgcg gcctctcggt gaatggtcac gtcgagcctc
    26581 ggcaagtctg cgacatcgag atcgatacca ccggccgctc gatgggcgag atccgcatgc
    26641 gcatcgcgcc gatcacgacg gccgccgatg gccagttcac gctcggtccc acgcagggcg
    26701 gcggcgtaga catcaacgcg cgttgcgcga gcggcgatca aggcacgaag cacgtcgagg
    26761 tcacggccaa cgtgacggac atcgtcgtcg aggtgaagcc cggcgcgtcg atcgcgggca
    26821 aggtcgtcga cggacaaggc aaacccgtcg tcgctgcgac ggtgatggcg tctccgatga
    26881 aggagaccga acgcacgacg atcgtcaacg gcatgctcgt cggcggcgtg cagagcgtga
    26941 ccaacggcaa aggcgagttc gagctccgcg gcctgtcggc cgggccctat cggatgcgcg
    27001 tcctcgatcg cggtcggccg ttgccgatga agagcgacgc gaaggtgacc gtcgcagcgc
    27061 tcgagaagaa gaccggcgtg acgctcaccg tcgagcgcgc agacggcgtc atccgcggtg
    27121 tcgtgaccgg ccccgatggc aagcccctcg ccgatgcgtg ggtatcgctg caacaaggca
    27181 tcgatgatct catcggcgat ccagacatgg ggcacggtga atcgcgcatg gtcacggtgg
    27241 agtccgtgca agtcggcgag ggcgagtccg gcgatggcgg cggctttgcg cccgtgctca
    27301 ccgacgcgac cggcaagttc gagatcgcga acctcccgcg cgtgccgtgg accgtcgttg
    27361 ccgaagcgca agcgggcaag ctgcgcggtc gccaagtcca tgtcacgccc gacgcgaaca
    27421 tcacgattca ggcgctcggc atcacggagc tcacaggcac ggtgaaacca gcgcccggtg
    27481 tcttcaccgt cgagctcgaa ggcccgacgc gcgcgcagcg cagcttctcg tctgcggacg
    27541 gctcgttctc gttcggtcgc gtcgatccag gcgagtacgc cgtgacggtg acatcgagcg
    27601 caggcaacgg cgcaggctcc gtcatcgtca agcccggcca acccgcgacg atcgacatcg
    27661 tgctcgccgc gaacgccacc gtgatcggca aggttgccga cccttcggga aagccattgg
    27721 gcggcgttcc cgtcgccgtc atccccgaca ataacgacgg caagctcagc gtgagcctgt
    27781 cgggcccacc accgaccacc aatcccgacg gcaccttccg tctagaagca aaggctggtc
    27841 gcagcgcagt catcgtcctc gtgccgccgc gtcccgcctc caagaaaggc ttgctcctag
    27901 aagcgggcaa gacattcgac gccggcacga tcaccgtcga cgcgagtcca cctccgaagc
    27961 cgtgatacgc tgacgccccg gcgatgagca aacaacggtg gaagttgttg ctctgcgcga
    28021 ttgcagcggc ggcaacgctc gtcactgcgc tgttcgtcag cgcgtggttc cacatggatg
    28081 tcatgggcag cgagatggag atcgatctcc gctctgcctc gatgtgcggc gccaacggcg
    28141 tatgcgtcgc ggtcccactc tccaagttcc gcggcttcta ctcgatcatg ggcaacgccg
    28201 cgttcttcgc ggccatcccg gtgctgatcc tcgtcgtcgt gcaatgcgca tcgcggctcg
    28261 tcacgggcgc cgcgagcagt gcggtcacca aagccggcta cggcctaggc atggtcgcgc
    28321 tgatcacggg cttcggcgcc ggctacttgt tcgcgccgca gtccgcaggc atcgatgaca
    28381 tcgccgtcat cacggtcaca cgcacgtggg cgccgctcat cttcgtcgtc ggcaatctgc
    28441 tcgcgctgtt cgcgatgtac ctcgcatcga gcgagtggct cgacgacgac gtcggcgagt
    28501 acaagcccat caagctcgat ggtggccgtc ttccggtcac gccgctcagc gtcaaccgca
    28561 tccacacgcc gcaaggctcg cagccgctgc aacgcatccc gacgccgcag ggctcgcaac
    28621 cgctccaacg tcccggcagc gacggtggcc gctcgcgcac gcccacgggc cagcaacgcg
    28681 ccggcagcga aggcggtcgg tcgcgaacgc cgacggggca gcacgccgtg agcgaaagtg
    28741 gtcgctcgcg aacgcccacg ggccaacagc gcgccatgac cgaaagtggt cgctcgcgaa
    28801 cgccgacagg ccagcagctc tccgaggatg tcgctcgacg gctgacgccg tcggagccta
    28861 tcgtgcgcat cgccgatcca tcgcggcgct tgaccccgtc gcagcaaacg cagcgcgtcg
    28921 atgatccggc acgtcgcttc acgccgtcgc agcagatcca gcgcgtcgat gatccgacgc
    28981 gtcgcctcac gccgtcgcag caaacgcagc gcctcgatga tccgacgcgt cgcctcacac
    29041 cgtcaggaca aatcccgcgc atcgacgatc cgtcgcgccc caagtctccg tcgcagccgc
    29101 ctgccatcgc gcggacctcg tcgccgtcag gcctccgctc gcgcactcca tcgcagcagc
    29161 cgctcggcat cgtcgaggtc gacgcgcgag cgaagtcgcc gtcgcagcag cccgtgctcg
    29221 cgcggacgac atcgccgtcg ggcatcgcga tcgatccggc ctcgcgtgcg cgcacgtcgt
    29281 cgtcggggcc aatcgatctt gcagctcgcc tcagcgcgac cgcaccgcaa ggcgtgctcg
    29341 agcctctcgg cggcagcgcc ggcgacgcgc cgatcgaatt ctcgattcgg ccgccgctgc
    29401 cacaacccga gcccgtaccg cccgatcaaa ttccggtcga tcctgccgcc ggcctgacga
    29461 ttcgcaagcg cccggcatcg gtcgcgccgc caccgctggc gccaccgctt gctgcggcgc
    29521 ccgcgattgc agcgtctccg cacgacgagg ttccgctctc gaaggcgaag accgtcgact
    29581 tgccggacag cgcattccag ccgccgatcg cgatcgagac gccgcccgaa tcgggcctcc
    29641 tgatccgcaa gaagtcgccg acgggcgcgc cccacgcgcc actctctgcg agcatgccgg
    29701 cgatcgcgct cgacggtatc gagggcctgg ccgagctcaa agacgagcta tcgaagccgc
    29761 acgcagcctc gcccgcctcg ccggcctcgc cggcctcctc gatcgaagaa gcgctcatcg
    29821 cgaatgcaga tgcgac
//
LOCUS       DATLBZ010000658         6454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7372855, whole genome shotgun sequence.
ACCESSION   DATLBZ010000658 DATLBZ010000000
VERSION     DATLBZ010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7372855"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VMZ53_32385"
     CDS             <1..1488
                     /locus_tag="VMZ53_32385"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUS33261.1"
                     /translation="TGGVVVGQDAKRYRLTEPQHVIHSAQRFIGQNIRAPLVQLALTG
                     IRYKVTEGPNQQPIVVVRDRRMTMPEISSHVLLHLKRCAERQLGESVNDAVVTVPANF
                     TDAQRQATKEAGRLAGLNVMRLINEPTAAALSYGFGRNQDQLVCVFDFGGGTFDVSIL
                     RINGEVFEVLASDGDYFLGGDDLDRALTEVLAAECNRTLGFDPRQQAVLMMKLMMGAE
                     AIKCHLSDNDAAEGEIDGIDLPNGATGSLPFALTRAQFEHLIAGYVNRTIEVTKQVLS
                     QARLTPNDISDVLCVGGSTRIPTVRQRLADTFRREPNVKINPDEVVAQGAAIQAGSLS
                     GSIISGTGMGARDAVMTEAHSPLYFGGAAPPKGPIARPVLLDVNPSTLSIQTAGGYAE
                     RILDKNSPVPIEKTRVFTTARDNQARVEIDCCRGESRRYAENEPLGNLLLDGLPPKPR
                     GDLKIEVCFRVDADGILHVSAQDADSGARQEAHMQVFGAPTAEGV"
     gene            1485..2147
                     /locus_tag="VMZ53_32390"
     CDS             1485..2147
                     /locus_tag="VMZ53_32390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33262.1"
                     /translation="MSTRSDLMLWAQRVIARKAGPAHEIMEVPADATPDQAQAAFHRI
                     ARTAHPDLHRKGLTAEELEMVTSAYAVVAGAYQTLRTQTMQTTRMKPVKPDDLAAVNA
                     PVVGRSGQTPPAGSPVARPGSPSVPPASRLTQGPARPTQPPPVAHVPATPAGPAPAGN
                     ASQSMSPKALVYYRKAELSLKRGDLKGAVLQLKLAIAADPASGFLRTALAEVELEVRK
                     GT"
     gene            2169..3302
                     /locus_tag="VMZ53_32395"
     CDS             2169..3302
                     /locus_tag="VMZ53_32395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HUS33263.1"
                     /translation="MPRITTGTAITGHAVWHPEQVLTNDELVVCFNEFVRRENAKYAD
                     EIAAGTRQPLKPSSSEFILKASGIKERYVEDKSGLLDPDRMCPNIPDRPETALSIQAE
                     YSINAAKLALARAGKTGEDIDLVVLGASNLQRLYPSLAIEVQEAIGARGWAYDMSLGC
                     SAATGATITATHAIQSGAATRALIVVPELTTGHMNWRERDSHFIFGDASVSLVLEKVE
                     DAKPGAWEILSTKMLSKWNATAIRNNYGYLDRCDPATKDNDSKLFHQKGRTVFKDVVP
                     LASAFIKEQLQAYGLEPSQVGRYWLHQANQKLNEVVAEWVLGRVPSRTEAPIILDEYG
                     NTASAGSLIAFSLHNEDLAAGTYGLMCSFGAGYSLGGLLMQRL"
     gene            3407..4804
                     /locus_tag="VMZ53_32400"
     CDS             3407..4804
                     /locus_tag="VMZ53_32400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33264.1"
                     /translation="MSSEIFRDRTEGASARRQDLLRRRRDEFVTMPHAIRRVYVARRA
                     RSGAAIAVWLTGVALLVAALSPKVAAFLTKGMPGINPAVLSTGVMGMWLLGIVAYFVA
                     RARCEHHFAVAISKTVLPGDDLDHDVERLANERPDEVARDMAHKLEVRSSAWPIAALA
                     MIGPATLVYIYYGMKAGGWPSIAAYEAALSLHAKKLAFVGGLGTFAAIFMTRRSLRLP
                     IVAPVAAALTLLFAAAAMAVSPWLTLVGVIGMSIAYIAYRLKRERAFLETDDPAAGTE
                     LFTWKGFWLQTRSAVSSVTSHLRPRQKKVIIAVLAAGAVFMVFGRGKHSDVKASKATV
                     VQVTSQEPAVIVNKEGAKFDVSPTGDGAFKIDFTTVDDNGVDVPLPGIAMIPRSWSAR
                     VSIQLLGPQLEGAVYATPFRTLDGTQQILGTSPTTFSRAACGEELVPLVLHITAPAGT
                     AYSLRVTPVLEPAGC"
     gene            complement(4773..5318)
                     /locus_tag="VMZ53_32405"
     CDS             complement(4773..5318)
                     /locus_tag="VMZ53_32405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33265.1"
                     /translation="MPPGLLLFLQHVGLALRLHSTMPVDPLEAVMHVQAAIEASTDQI
                     SPEVLLAIAYVESRYEPLALSRIEGKKRKLGMYLPDEPPKNWKQGTSLHCGPLQTYAL
                     TWDECLGMRRSLHTAYAAGAKQLSTWLRDRRVRGDLTRALAGYGCGNHGVKTGKCNRY
                     PGRVLWQARRIASSLPARGRA"
     gene            5513..>6454
                     /locus_tag="VMZ53_32410"
     CDS             5513..>6454
                     /locus_tag="VMZ53_32410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HUS33266.1"
                     /translation="MLSDAAATPGGGEAILARALERFGHPALRPGQADVIADIFAGRP
                     VIAVMPTGAGKSLCYQLPAIVLGERGGVTLVVSPLIALMKDQVDALRARGIPACALTS
                     AAGAEEQRDIIDGIRAGAYTLVYVAPERFRSPRFVDALRASASRIALVAIDEAHCISE
                     WGHDFRPDYRRLGQVVAELQPPRLAAFTATATPEVRADIGVQLGLEDPRLHVRGFDRP
                     NLFYSVVKAGGAADKTEQLAELVRMREGGVALVYAATRKNAEQYSTALRQAGMRSRVY
                     HAGLEDEVRVKAQDVFMAGQLDVIVATNAFGMGVDKSD"
BASE COUNT         1079 a         2179 c         2104 g         1092 t
ORIGIN      
        1 acgggcggcg tcgtcgtcgg ccaggacgcc aagcgctacc ggctcaccga gccacagcac
       61 gtcatccatt cggcccagcg cttcatcggc cagaacatcc gtgcgccgct cgtccagctc
      121 gcgctgacgg gcatccgcta caaggtcacc gaggggccga accagcagcc gatcgtcgtc
      181 gtgcgcgatc gccgcatgac gatgccggag atcagctcgc acgtgctgtt gcacctgaag
      241 cggtgcgcgg agcgccagct cggcgagtcg gtcaacgacg cggtcgtcac ggtgccggca
      301 aacttcaccg acgcccagcg gcaggccacg aaggaagccg ggcgcctcgc cggcctcaac
      361 gtgatgaggc tcatcaacga gccgacggcc gctgcgctct cctacggatt tggccgcaac
      421 caggaccagc tcgtctgcgt gttcgacttc ggcggcggca cgttcgacgt gtcgatcctg
      481 aggatcaacg gcgaggtgtt cgaggttctc gcgtccgacg gcgactactt cctcggcggc
      541 gacgatctcg atcgcgcact gacggaggtc ctcgcggccg agtgcaatcg cacgctcggc
      601 ttcgatccgc ggcagcaggc ggtcctcatg atgaagctca tgatgggagc cgaggcgatc
      661 aagtgtcacc tctccgacaa cgacgctgcc gaaggcgaga tcgacggcat cgatctgccg
      721 aatggcgcca ccggctcgct gcccttcgcg ctgacgcgcg cgcagttcga gcacctgatc
      781 gcgggctacg tgaaccgcac gatcgaagtg acgaagcagg tcctctcgca agcgcgcctc
      841 acgccgaacg acatcagcga cgtgctctgc gtcggcggct cgacacgcat tccgacggtg
      901 cgccaacgcc tcgccgatac gttccgccgc gagccgaacg tgaagatcaa tcccgacgag
      961 gtcgtcgcgc aaggcgccgc gattcaggcg ggctcgctgt ctggctcgat catctcgggc
     1021 acgggcatgg gcgcgcgcga tgcggtgatg acagaagcgc actcgccgct ctacttcggc
     1081 ggcgcggctc ctccgaaagg cccgatcgct cgccccgtgt tgctcgacgt caatccgtcg
     1141 acgctgtcga tccagaccgc gggcggttac gccgagcgca ttctcgacaa gaactcgccg
     1201 gtgccgatcg agaagacgcg cgtgttcacg accgcccgcg ataatcaggc gcgcgtcgag
     1261 atcgattgct gccgcggtga atcgcgccgc tacgccgaga acgagccgct cggcaatctg
     1321 ctcctcgacg gtctcccgcc caagccgcgt ggcgatctga agatcgaagt ctgcttccgc
     1381 gtcgacgcgg acggcattct ccacgtgagc gcacaagacg ccgactcggg tgcccgccaa
     1441 gaagctcaca tgcaggtctt cggcgcgccg accgccgagg gcgtgtgagt actcgctccg
     1501 atctcatgct gtgggcgcag cgcgtgatcg cccgcaaggc cggaccggcg cacgagatca
     1561 tggaggtccc ggcagacgcg acgcccgatc aagcgcaggc cgcgtttcac cgcattgcgc
     1621 gcaccgcgca tcccgatctc catcgcaaag gcctcaccgc cgaagagctg gagatggtga
     1681 cgagcgcata cgccgtcgtc gccggtgcgt atcaaacgct gcgcacccag acgatgcaga
     1741 cgacgcgcat gaagccggtc aagcccgacg atctggctgc ggtgaacgcg cctgttgtcg
     1801 gaaggtcggg ccagacgccg cccgcaggct cgcccgttgc gcggccgggg tccccgtcgg
     1861 tgccgccagc aagtcgcctc acgcaaggcc ccgcccgccc gacgcaaccg ccaccggtcg
     1921 cgcacgttcc cgcaacaccg gccggaccag cgcccgcggg taatgcgtcg cagtcgatgt
     1981 cgccgaaggc tctcgtctat tatcgcaagg cggagctgag cctgaagcgc ggcgacctca
     2041 agggcgcggt tctgcagctc aagctcgcga tcgccgctga cccagcttcg ggctttctcc
     2101 gtacggcact tgccgaggtc gagctagagg tccgcaaggg cacctgactt cgtgtatacc
     2161 ccgcgacgat gccgcgcatc acgacaggta ccgcgatcac cggtcacgca gtctggcacc
     2221 ccgagcaggt cctcaccaac gacgagctcg tcgtctgttt caacgagttc gttcgccgcg
     2281 agaacgcgaa gtacgccgac gagatcgcgg ctggcacgcg gcaaccgctc aagccgagct
     2341 cgagcgagtt catcctcaag gcgtccggca tcaaagagcg ttacgtcgag gacaagtcgg
     2401 ggcttctcga tcccgatcgc atgtgcccga acattccgga tcgtccggag accgcgctca
     2461 gcatccaggc cgaatactcg atcaacgcgg cgaagctcgc gctcgcacgc gcgggcaaga
     2521 ccggcgagga catcgatctc gtcgtgctcg gcgcgtcgaa tctgcagcgc ctctatccgt
     2581 cgctcgcgat cgaggtccaa gaagcgatcg gcgcgcgcgg ctgggcgtac gacatgtcgc
     2641 tcggttgttc cgccgccacc ggcgcgacga tcaccgcaac gcatgcgatc cagagcggcg
     2701 ccgcgactcg cgcgctcatc gtcgttcccg agctcacgac cggtcacatg aactggcgcg
     2761 agcgcgacag ccacttcatc ttcggcgatg catcggtgtc gctcgtgctc gagaaggtcg
     2821 aggacgcgaa gccgggcgcg tgggagatcc tgtctacgaa gatgttgtcg aagtggaacg
     2881 cgacggccat ccgcaacaac tacggttatc tcgatcgctg cgatcccgcg acgaaggaca
     2941 acgacagcaa gctcttccac cagaagggcc gcacggtctt caaggatgtc gtgccgctcg
     3001 cgtcggcctt catcaaggag cagctccagg cgtacggtct cgagcccagt caggtcggac
     3061 gttactggct gcaccaggcc aaccagaagc tcaacgaggt cgtcgcggag tgggtgctcg
     3121 gccgggtgcc ctcccgcacc gaggcgccga tcatcctcga cgaatatggc aatacggcct
     3181 cggcgggatc gttgatcgcg ttctcgctgc acaacgagga cttggcggcg ggcacctacg
     3241 gcctcatgtg ctcgtttggc gccggttact cgctcggcgg cctcttgatg caacgcctgt
     3301 aaggtcgggt gtgtcccctc ccgcttgggg gcagctcccg ggaataaacg gcagctaaac
     3361 gatttagcgg caagaccttt ttcgctttgc tagcgttgaa ggggtcatga gcagcgaaat
     3421 cttccgcgac cgtaccgaag gtgcctccgc tcgccgccag gatctgttgc gccggcgccg
     3481 cgatgagttc gtcacgatgc cgcacgcgat ccgtcgcgtg tatgtcgcgc gccgcgctcg
     3541 gagtggtgca gcgatcgctg tgtggctgac gggcgtcgcg ctgctcgtcg ctgcgctctc
     3601 gccgaaggtc gctgcgtttc tgacgaaggg catgcccgga atcaaccctg ccgtgctgtc
     3661 gacgggcgtg atgggaatgt ggctcctcgg catcgtcgcg tacttcgttg cgcgcgctcg
     3721 ctgcgagcat cacttcgcgg tcgcgatcag caagacggtg ttgcccggcg acgacctcga
     3781 ccacgacgtc gagcgcctcg cgaacgagcg gcctgatgaa gtcgcgcgcg atatggcgca
     3841 caagctcgag gttcgctcct cggcgtggcc gatcgcggcg ctcgccatga tcggtcctgc
     3901 gacgctggtc tacatctatt acggcatgaa ggcgggcggc tggccgtcga tcgctgcata
     3961 cgaagcggcg ctctcgctgc acgcgaagaa gctcgcgttc gtcggtggcc tcggcacgtt
     4021 cgccgcgatc ttcatgacgc gtcgctcgct ccgtctaccg atcgttgcgc cggtcgcggc
     4081 cgcgctgacg ctcctgttcg cagcggcggc gatggcggtt tcaccgtggc tcacgctcgt
     4141 cggtgtcatc ggcatgtcga tcgcgtacat cgcgtaccgc ctgaagcgcg agcgcgcatt
     4201 cctcgagacg gatgatccgg cggcgggcac ggagctgttc acgtggaagg gcttctggct
     4261 gcagacgcgt agcgcggtct cctcggtgac gagccatctg cgcccgcgcc agaagaaggt
     4321 catcatcgcg gtcctcgcgg caggcgccgt gttcatggtg ttcggtcgcg gcaagcactc
     4381 cgacgtcaag gcgtcgaagg cgacggtcgt ccaggtcacg tcgcaagagc cagccgtgat
     4441 cgtcaacaaa gagggcgcga agttcgatgt ctcgcctacg ggcgacggtg cgttcaagat
     4501 cgacttcacg accgtcgacg acaacggcgt cgatgtgccg ctgcccggca ttgcgatgat
     4561 tcctcgctcg tggagcgcac gcgtctcgat ccagctgctc ggcccgcagc tcgagggcgc
     4621 ggtctacgcg acgccgttcc gcacgctcga tggcacccag cagattctcg gcacgtcgcc
     4681 gacgaccttc tcgcgcgcgg catgcggcga ggagctcgtt ccgctcgttc ttcacatcac
     4741 ggcgcccgcg ggcaccgcgt actcgctgcg cgttacgccc gtcctcgagc cggcaggctg
     4801 ctagcgatcc ggcgcgcctg ccagagcacg cgccctggat agcgattgca cttgcccgtc
     4861 ttcacgccgt gattgccgca tccgtagccg gcgagcgcac gcgtgagatc gccgcgaact
     4921 cgacgatcgc gtagccacgt cgacagctgc ttcgcgcccg ccgcgtacgc cgtgtgaaga
     4981 ctgcgccgca tgccgaggca ctcgtcccac gtcagcgcgt acgtctgcag tggaccgcag
     5041 tgcagcgacg tgccctgctt ccagttcttc ggcggctcgt cggggagata catgccgagc
     5101 ttgcgcttct tgccttcgat tcgcgacagc gcgagcggct cgtagcggct ctcgacgtac
     5161 gcgatcgcga gcagcacttc gggcgagatt tgatcggtcg acgcttcgat cgcggcctgc
     5221 acgtgcatga cagcctcgag cggatcgacc ggcatcgtcg agtgcaggcg cagggcgagc
     5281 ccgacgtgct ggaggaacaa caggaggccg ggcggcatgc ggggttctct gagcaagttc
     5341 cgggcgcact cccggttatt gaagcctgtg gatggcctgt gaaaagccga ctccggagcg
     5401 agcgctcccg tccattgcgg cgcagtcgca cactgaatgg tgtttggcgg cgcgtactgc
     5461 cccagtccta gagtcgacaa aaaggtcgga cccgtttggt acggtgtgcg cgatgttgag
     5521 tgatgcggcg gctacgccgg gaggcggcga ggcaatcctc gcgcgcgcgc tcgagcgttt
     5581 cggtcaccca gcgctgcgcc cgggccaagc agacgtcatc gccgacatct ttgccggtcg
     5641 tcctgtcatt gcagtcatgc cgacgggcgc gggcaagtcg ctctgctatc agctgcccgc
     5701 gatcgtgctc ggtgaacgcg gcggcgtgac gctcgtcgtg tcgccgctga tcgcattaat
     5761 gaaggatcaa gtcgacgcgt tgcgcgcgcg cggaattccc gcgtgtgctc tgacgtcggc
     5821 agccggcgcc gaagaacagc gcgacatcat cgacggcatt cgcgcgggtg catacacgct
     5881 cgtgtacgtc gcgccggaac gctttcgcag cccacgcttc gtcgacgcgt tgcgagccag
     5941 cgcatcacgc atcgcactcg tcgcgatcga tgaagcgcac tgcatctcgg agtggggcca
     6001 cgatttccgg ccggactatc gccgcctcgg tcaggtcgtc gccgagctgc agccacctcg
     6061 cctcgccgcc ttcaccgcga cggccacgcc cgaagttcgc gccgacatcg gagtccagct
     6121 cggcctcgaa gatccgcgcc tccacgttcg cggcttcgat cgtccgaatc tgttttattc
     6181 ggtcgtgaaa gcaggcggcg ccgcggacaa gaccgagcag ctcgcggagc tcgtgcgcat
     6241 gcgcgagggt ggcgtcgcac ttgtctacgc agccacgcgc aagaacgcgg agcaatactc
     6301 gacggcgctt cgccaagcgg gcatgcgctc gcgcgtctat cacgcaggtc tcgaggacga
     6361 agtgcgcgtg aaggcgcaag acgtgttcat ggccggccag ctggatgtga tcgtcgcgac
     6421 gaacgcgttc ggcatgggcg tcgacaagag cgac
//
LOCUS       DATLBZ010000659         4765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3665850, whole genome shotgun sequence.
ACCESSION   DATLBZ010000659 DATLBZ010000000
VERSION     DATLBZ010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3665850"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /locus_tag="VMZ53_32415"
     CDS             complement(<1..1725)
                     /locus_tag="VMZ53_32415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33267.1"
                     /translation="MQRLISLGILLASSVALAEPTSGVDTVMYRPSYDTNGIFALEGA
                     RLMPKRDLAIKLQLGYAQSPIDVSVPGIGAMAGDTNKESILDYLVALDMTFGMTLTSK
                     VAIGMDIGSYRTATGSGYGVRGRYVSGGTISMPSTGLIALRPLSNIDQSANPDDPGAY
                     LGDGRSGPLDVRAGLKIALFEDPKTALALVGSVFLPFGDDYMLLGDRNLVYEPKLAFE
                     WRPDRVHATRFLANLGARIRNRTILEGYDTAATMPTSADAKVFLDVGSEMVAGVGGQY
                     ELTPRAVLGLEAQAFVPLPSSIGYGKCRRFNGLECSTIDSDDYWAGAKAGDLSVLAML
                     GMLLRVSADVSANVMISSGPVGARGDDFRITTGLVWSPQPAGVAAPGRNDHDGDGIPD
                     SVDACADEAEDKDGFQDEDGCADADNDGDGIPDGNDDCANEPEDKDGFQDGDGCPERD
                     NDADTVPDAADKCPNDPEDVDGFEDDDGCPDQDNDGDGFPDPSDKCPNDPETVNSFED
                     DDGCPDVRATTGPEERPDRIDLKGQQVGFDKQNKLTAAGKSLLNQVATIIKNRKLTIR
                     IEVHVARGT"
     gene            complement(1713..2831)
                     /locus_tag="VMZ53_32420"
     CDS             complement(1713..2831)
                     /locus_tag="VMZ53_32420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33268.1"
                     /translation="MRNTPLLITLFASLTATAAGCAQTECATGTIERDGTCQPATAMS
                     DPSQCGPGTELQGDQCVPVVAETHCDPATTIEQVNEDGTITCVGNGTGGDCSTPIACP
                     TPSGSATKLTICGQIFDFENNSKFADSTGAGNCDASMPAASGPCALQIIAYDAIAFGT
                     NPATAAPLAVDSVTIDKCGRYRVAGIETNGTGPFIGLGIDDAGQPLGPAGVTVTVGIA
                     APKAEGPVIQGFEGWIVKASTTALWAANGGPPLSGGIYAPVYRKHKKGVGDPFENQNG
                     VTVVKASDFNPVGAQDHYFPPTDPNRQNTDTAATATGVNGTALFTGAKLSDGLYSGLG
                     GLGAGCTWDTHAGASLPGIVFIQIFRKHDAAIGGPCND"
     gene            complement(2840..3823)
                     /locus_tag="VMZ53_32425"
     CDS             complement(2840..3823)
                     /locus_tag="VMZ53_32425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33269.1"
                     /translation="MRTTAIALLLLGGCIDEFHGSNVQIDLSATTPVQASVGVNPTTE
                     QLPDNIHFTLYAFEQDDANGTPVGRLFEVETFEIHRIVDLASPCSIDVGEHVPFPGLH
                     VSQYAAQVMAKYGWTSMTPPPTAGETEKVEVATAIQRQRNIEAQAGNQGLKVVSGASN
                     GGYLEVGADCSDTTKLPPPMCTDADSNARRLAMCQEAWSHDPEYFEGTDRVLTVPLNG
                     TTHGMVTGSNPVNLAPVGGAQFFVDESLDDFQGYAIYWQYDDANHDGVPDYPASLPMA
                     DRRELGTQLLYGKPSAPTRGVIHVDMSNRAEPSLTAELAIFASLDDDEVHF"
     gene            complement(3865..>4765)
                     /locus_tag="VMZ53_32430"
     CDS             complement(3865..>4765)
                     /locus_tag="VMZ53_32430"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HUS33270.1"
                     /translation="DDRPSRFTLGFGVWNTFGGDVAFPKTGMPALDTTRDIVVEANVA
                     AAMHVSDRFAVGAAVRLGIGFFKVESTDAPFDADLSASGVGVAMTWGALFRPTDAVRV
                     GLTWRSPLRVTTKGSGTISFGGPAERHDVRHRQNWPQQVSLGVGWQGTPQLKLATQID
                     WTEWSQVDEIRVEFPASVLPAQIYPEYWDDSWTLRAGGEYAVSSVLALRAGTYFDTSA
                     VPDRTIERQYLDTDKLGLSGGASLRAGGWRFDAALDGVIPTTRTVENNAMDVMGFTPL
                     VNKAPGDYRGTLITFEVAAARPF"
BASE COUNT          758 a         1555 c         1580 g          872 t
ORIGIN      
        1 cgtgccgcgc gcgacgtgga cctcgatgcg gatcgtcagc ttgcggttct tgatgatcgt
       61 cgcgacctgg ttgagcagcg acttaccagc cgccgtcagc ttgttctgct tgtcgaagcc
      121 gacctgctgg cccttgaggt cgatgcgatc cggacgctcc tcgggaccgg tcgtcgcgcg
      181 aacgtcggga cagccgtcat catcctcgaa gctgttcacg gtctccggat cgttcgggca
      241 cttgtcgctc ggatcgggga agccgtcgcc gtcgttgtcc tgatcggggc agccgtcatc
      301 gtcctcgaag ccgtcgacgt cttcgggatc gtttggacac ttgtcggccg cgtcgggaac
      361 ggtgtccgca tcgttgtcgc gctccgggca gccgtcgccg tcctggaagc cgtctttgtc
      421 ctcgggctcg ttcgcgcaat cgtcgttgcc gtcggggata ccgtccccgt cgttgtctgc
      481 gtcggcgcag ccgtcttcat cttggaagcc gtctttgtct tcggcttcgt cggcgcatgc
      541 atcgacggag tccgggatgc cgtcgccgtc gtgatcgttg cgacccggcg cagcaacgcc
      601 tgccggttgc ggcgaccaca cgaggccggt cgtgatgcgg aagtcatcgc cgcgcgcacc
      661 gacggggccg ctcgagatca tgacgttcgc gctgacgtcg gcggagacgc gcagcagcat
      721 gccgagcatc gcgaggaccg agaggtcgcc ggctttggcg cccgcccagt agtcgtcgga
      781 atcgatcgtc gagcactcga gtccgttgaa gcgacggcac ttgccgtagc caatgctcga
      841 cggcagcggc acgaatgcct gcgcctcgag accgagaacc gcgcgcggcg tgagctcgta
      901 ctggccaccg acgccggcta ccatctcgga gccaacatcg aggaagacct tcgcatcggc
      961 cgaggtcggc atcgtcgctg ccgtgtcgta gccctcgagg atcgtgcgat tgcggatgcg
     1021 cgcgccgagg ttcgcgagga accgagtcgc atgcacgcga tcgggacgcc actcgaacgc
     1081 gagcttcggt tcgtagacga ggttgcgatc gccgaggagc atgtagtcgt cgccgaaagg
     1141 caggaacacc gagccgacga gcgcaagcgc ggtcttggga tcttcgaaga gtgcgatctt
     1201 tagaccggcg cgcacgtcga gtggccccga gcgaccgtct ccgagatacg cacctggatc
     1261 gtcgggattc gcggactgat cgatgttcga gagtgggcgc aacgcgatga ggcccgtcga
     1321 cggcatcgag atcgtgccgc ccgaaacgta gcgaccacgc acgccgtatc cgctgcccgt
     1381 cgcggtgcgg tacgagccga tgtccatgcc gatcgcgacc ttcgacgtca gcgtcatgcc
     1441 gaacgtcatg tcgagcgcga cgaggtagtc gaggatcgat tctttgttcg tgtcacccgc
     1501 catcgcgccg atgccgggca cggacacatc gatcggcgat tgcgcgtagc cgagctgcag
     1561 cttgatcgcg agatcgcgct tcggcatgag tcgcgcgcct tcgagcgcga agatgccgtt
     1621 ggtgtcgtac gacggccggt acatcaccgt gtcgacgccg ctggtcggct cggccagtgc
     1681 gaccgacgac gcgagcagga tgccgaggct gatcagtcgt tgcatggtcc gccgattgcc
     1741 gcgtcatgct tgcggaagat ttggatgaag acgatgccgg ggagcgatgc accggcatgc
     1801 gtgtcccacg tgcaacccgc gccgagcccg ccgaggcccg agtagaggcc atcgctgagc
     1861 ttcgcgccgg tgaacagcgc ggtgccgttg acgcccgtcg ccgtcgcggc ggtgtccgtg
     1921 ttctggcggt tcggatccgt cggcgggaag tagtgatctt gtgcgcccac cgggttgaag
     1981 tcgctggcct tcacgaccgt gacgccgttc tggttctcga acggatcgcc aacacccttc
     2041 ttgtgcttgc ggtacacggg cgcgtagatg ccgcccgaca gcggaggccc gccgttcgcc
     2101 gcccacagcg ccgtcgtcga cgccttgacg atccagcctt cgaagccttg gatcaccggg
     2161 ccctcggcct tcggtgcggc gatgccgacg gtgaccgtca cgcccgctgg cccgagcggc
     2221 tgccccgcat cgtcgatgcc aaggccgatg aacggaccgg tgccgttggt ctcgataccg
     2281 gcgacgcgat agcgaccgca cttgtcgatc gtcaccgagt cgacggcgag tggcgcggca
     2341 gtcgccgggt tcgtgccgaa cgcgatcgcg tcgtatgcaa tgatctgcag cgcgcacggt
     2401 ccgctcgccg cgggcatcga cgcatcgcag ttccctgcgc cggtcgagtc tgcgaacttc
     2461 gagttgttct cgaagtcgaa gatctggccg cagatcgtca gcttcgtcgc gctacccgac
     2521 ggcgtcggac acgcgatcgg agtgctgcag tcgccgccgg tgccgttgcc gacacacgtg
     2581 atcgtgccgt cctcgttgac ttgctcgatc gtcgtcgcgg gatcgcagtg agtctcggca
     2641 acaaccggca cgcattgatc gccttgcagc tcggtgccgg gaccgcattg cgacgggtcg
     2701 gacatcgcgg tagcgggctg gcacgttccg tcgcgctcga tcgtgcccgt cgcgcactcg
     2761 gtttgcgcac aacctgcggc ggtggccgtc agcgacgcga acaacgtgat gagcagtggg
     2821 gtattccgca tcggcgctcc tagaagtgaa cctcgtcatc gtcgaggctt gcgaagatcg
     2881 ccagctcggc ggtgagcgag ggctcggctc ggttcgacat gtcgacgtgg atcacgccgc
     2941 gcgtgggtgc cgacggtttg ccatacaaga gttgcgtgcc gagctcgcgg cggtccgcca
     3001 tcggcaggct cgcgggatag tcgggcacgc cgtcgtgatt cgcgtcgtcg tactgccagt
     3061 agatcgcgta gccctggaag tcgtcgagcg attcgtcgac gaagaactgc gctccgccga
     3121 ccggcgcgag gttcacgggg ttcgagcccg tcaccatgcc gtgcgtcgtg ccgttgagcg
     3181 ggacggtgag cacgcggtcg gtgccctcga agtactcggg gtcgtgtgac cacgcctctt
     3241 ggcacatcgc gaggcgccgc gcgttggagt cggcgtcggt gcacatcgga ggcggaagct
     3301 tcgtcgtatc gctgcagtct gcaccgacct cgagatagcc gccgttcgac gcgccgctca
     3361 caaccttgag gccctgattg ccggcctgcg cctcgatgtt gcgttggcgt tggatcgcgg
     3421 tcgcgacttc gaccttctcc gtctcgcccg ccgtcggcgg tggcgtcatc gatgtccagc
     3481 cgtacttcgc catcacctgc gccgcgtact ggctcacgtg cagaccagga aacggcacgt
     3541 gctcgccgac atcgatcgag catggcgacg ccaagtcgac gatgcgatgg atctcgaatg
     3601 tctcgacttc gaacagacgt ccgaccggcg taccgttcgc atcgtcttgc tcgaatgcgt
     3661 agagcgtgaa gtggatgttg tcaggaagct gttcggttgt cgggttcacg ccgacggacg
     3721 cctgcacggg cgtcgtcgcc gacagatcga tttgcacatt cgatccgtgg aactcgtcga
     3781 tacaaccgcc gagcaagagc agagctatcg ctgtggtgcg cacgtccccg actcgtaatg
     3841 ttagccagtt ggccaaccta aagtctagaa aggccgtgcc gctgcgactt cgaacgtgat
     3901 gagagtgccg cggtagtcgc ccggagcttt attcaccaaa ggcgtgaacc ccatcacgtc
     3961 catggcgttg ttctcgacgg tgcgggtcgt cgggatgacc ccgtctagcg ccgcatcgaa
     4021 ccgccagccg cctgcgcgca ggctcgcacc tccagacagc ccgagcttgt cggtgtcgag
     4081 gtattggcgc tcgatcgtgc gatcgggtac cgccgaggta tcgaagtagg ttccggcccg
     4141 aagcgcgagc acgctcgaga ccgcgtattc gccaccggcg cgcagggtcc agctgtcgtc
     4201 ccagtactcg ggataaatct gagccggcag gacgctcgcc ggaaactcga cgcggatctc
     4261 gtcgacctgc gaccactccg tccagtcgat ttgcgtggcg agcttcagct gcggggtccc
     4321 ctgccagccg acgccgagcg agacctgctg cggccagttt tgccggtgtc gaacatcatg
     4381 gcgttcggcc gggccgccaa acgagatcgt tccgctgccc ttcgtcgtga cgcgcagcgg
     4441 cgaacgccac gtcaatccaa cgcgcactgc atcggtcggc cgaaacagcg cgccccacgt
     4501 catggcgacg ccgacaccgc tcgccgacag atccgcgtcg aacggcgcat cggtcgactc
     4561 gaccttgaaa aagccgatac caagacgcac ggccgccccg acggcaaacc gatcgctgac
     4621 gtgcatcgcc gccgcgacgt tcgcctcgac gacgatgtcg cgcgtcgtat cgagcgcggg
     4681 catgcccgtc ttcggaaacg cgacgtcgcc gccgaacgtg ttccacacgc caaacccgag
     4741 cgtgaagcgg ctcggccgat cgtcg
//
LOCUS       DATLBZ010000660        24593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6475782, whole genome shotgun sequence.
ACCESSION   DATLBZ010000660 DATLBZ010000000
VERSION     DATLBZ010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 24593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24593
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6475782"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VMZ53_32435"
     CDS             <1..817
                     /locus_tag="VMZ53_32435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33271.1"
                     /translation="PQGQYPQGQYPQGQYPQGQYPQGQYPQGQQPYPYPQQPYPYPQQ
                     PYPYPQQPYPYPPQQQPAPQPQPSPEETGPKRNHLEVGLLTGFAAPMNRRNEQVPALA
                     GLSVSAGYVFGPVGVWLDFDSLANTDATHGTFLISGSFAGKAGEKLSIGGRFGLGSTL
                     VNFHEPAFDDVQASNIRFEATLDYALAASWSLWARPFSIDIMTASELGGPITTLQMRV
                     GVAFTFGAHRRAAVAAQQPAPAPQPAPAPQPAPAPQPAPAQPAQPTATQARNP"
     gene            814..1512
                     /locus_tag="VMZ53_32440"
     CDS             814..1512
                     /locus_tag="VMZ53_32440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33272.1"
                     /translation="MSWRNLSIALAMVCLFQWWRACNRPAPVAADEHAECESEPAQNR
                     VASSSSDSPSSSTARAKHNAGNDDAPDEAPPEGPRLGQIKLPAWTLWLAPHPGENMLD
                     YRDRMVPLAQTMLKPQRDRVARSRDDLAKQIGLDSQQRAQLDGAVADAATQIEDKMMN
                     AFMSGDLNPATFKPMTGVSLARDVLDVVDQANRRFTQTLREDQKATLAKHPFDFADYL
                     LFSARWEDALGVTN"
     gene            1545..2384
                     /locus_tag="VMZ53_32445"
     CDS             1545..2384
                     /locus_tag="VMZ53_32445"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HUS33273.1"
                     /translation="MRLVVLLLALAACAAKQECKLDPIPPGAHGGAFLWKVQRGGDVV
                     WLYGTIHDSGLDAVPRVALKALEGSVRFVSELGDSEPDREVFIKYARVDHGPGIDTRL
                     PPGDWYDLRDALLGSIKENDLRRAAPWYAMTLLSTKMAPTKEQSMDVLLGKRARELAM
                     PVEPLETWDEQLSALQTAVDIEDLKDAIHARHTMTCDFSRMRASYEAGDTKMMEALLV
                     VPKTQATMLDARNARWLPKIEGYVARGGAFVAVGLGHLLGPKGLPALLEKDGYTVERI
                     GIE"
     gene            2399..3373
                     /locus_tag="VMZ53_32450"
     CDS             2399..3373
                     /locus_tag="VMZ53_32450"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HUS33274.1"
                     /translation="MLAQEAAEAAAGARRQIEVCAPVFADLGKRLRANPPRLVVTCAR
                     GSSDHAATYGKYVVETAARTVVASVGPSVASMYHRAPQGLDGALFIAASQSGRSPDLV
                     ELTQAARKAGALVVGFINAEGSPLAEACDVVVPLCAGEEKAVAATKSFLLTGVAFLQL
                     AAHWTEDIALHDAVARAPDALDAAAAQPWPLAQLAGAESLFVVGRGLGLGPAQEIALK
                     LKETCRLHAEAFSSAEVLHGPMALVREGFPVVGVVQDDETAESTREVLQHIAALGATV
                     HLHVAHEAAHLAPLCQVQSFYLGLPALAAARGLDADLPAHLHKVTETR"
     gene            3573..4295
                     /locus_tag="VMZ53_32455"
     CDS             3573..4295
                     /locus_tag="VMZ53_32455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33275.1"
                     /translation="MRPSIFEGVAVAASLCLVGCLGDPASPTTTTLTAREAFDQRAWP
                     SLSTCAGCHGNQPAIDFLAPGTADGAYTTVFAFQPPVIDIEVPSSSLLLTMGKHTGPA
                     LSADQAAGVLEWLEAERNERMPETGNTVRVGPVLPTPGTAITLDLGINGAQLTLMPEA
                     SEAGLYFSHVTIAGGSGVHLTHPLFVSRPPKPILDPVDRFGALDAKVAAGGQLELGPV
                     WFLDFTTTDYLSIHFSTLEAPQ"
     gene            4292..5416
                     /locus_tag="VMZ53_32460"
     CDS             4292..5416
                     /locus_tag="VMZ53_32460"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HUS33276.1"
                     /translation="MKRYFLLAALVSPLAGCAAEPGPSGPPHDEWDDKLADRVVDYGA
                     ALRIASLRLTGELPTLADIDAIANAPDEASQKAAYESTIQRYIASPRFARQMFRFSQD
                     MLRLGDAPETDTAPAFLAQLIVENRSYLDVLTATTGTCPTFDVRTGVFTAGNCNNGTQ
                     AAGLLTHPGMLAANFSNLAFRRVRWVQETFACTKFPAEIAKTPTDVGGATPYTGTFPF
                     DSISGTPTGRVNFKDTSSVICANCHSNLNHLAPLFAHYDETGQFQTGFAVPVPLPNNP
                     LVAMSDYLPVGEPTAWRFGTPVSDMRSLGTAMAADPAIAECAIARVWNWALGRPDIVD
                     ASAKVPSTTIESQVADFKAGGYHLRDAMYRVFTSDDFVRF"
     gene            5427..6023
                     /locus_tag="VMZ53_32465"
     CDS             5427..6023
                     /locus_tag="VMZ53_32465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33277.1"
                     /translation="MRYALLLLIAGCTTEIVDSTTTGHAEDPYTSLEREQREGPPRYT
                     SRLHSCAKMRVATLGNVLASRGVDLAATPELSAGRIYRTSTIALGAANYAARTRENVE
                     LGVATASKIFDIYVQAAPEIIAAMPSRPECQRNGQPAQLFDSSNRCVESGFSCLIGTR
                     ATPEHLAVCNETVKRAADPESGKRLAVALLAAAAHTCE"
     gene            6032..7435
                     /locus_tag="VMZ53_32470"
     CDS             6032..7435
                     /locus_tag="VMZ53_32470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33278.1"
                     /translation="MTRDRDRDITRRALIQWGVAAGAALGVSKSRLAEIFERTGDKQL
                     AHAASSAPTKRSVHIRAGNGGLAWFTLLWPHNDIAAAAPNNPVTTWPHAPAVTRKLTG
                     TGGQLTVGPNTPFVGLEGDTATVTAFMAGNNEAHVQNPNSIARSLNGNSMFAIASVLQ
                     QASPTVIPVITVDDVALGTAPGSPRAAVVPTGLDIVGLFNSAASRAGGLLERAKYAGH
                     ADLYRSHFETLAQLNRASGRSTTRDSYATARSAAGFLGQNLAAKLAVQPADEIAYGIK
                     NADGTPSTMRQEVVAIARTLIVTAKAFQLHLTSSVVLPGVRDDPHDAFTNTAKANTAR
                     DLKTVLDAFMRDLKNRNDDATGAPLSEDIVITIEGDTPKTPLDRTNWLDDTPGNSNWM
                     YVYSGGLIKTGWLGGIDRNRGVKGFDPATGAPTPYDGDLGAQAAVAAVAYAITKGDKR
                     RVQDFSRVDISGIVNAV"
     gene            complement(7432..9138)
                     /locus_tag="VMZ53_32475"
     CDS             complement(7432..9138)
                     /locus_tag="VMZ53_32475"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HUS33279.1"
                     /translation="MKQLWVLLALMIAACGPGGRNGNGDDDDDGNDTCPRCSDDKTAV
                     ISCDGTAKACAPDQLCSNGECMNGCAAAENNHASVGCDYYAVDMDAADGPPRDACFTV
                     FVANTSRGQAHIDVDWGGQGIDLSKFAKLPQGSGTSLTYTNYDPSTGLAPGQVAILFL
                     AYAPAGSPLMGNVTCPVPAAIGTDAQIAGNGIGKAFHIVTDLPVVAYQMLPFGGGKAA
                     ATGASLLLPTSAWGNNYVGVTAYDTLDGSAPPIQIAQGPSMNIVAKEDGTTVTMRPMS
                     NVTGGNGVPPATMGQSMSFQLNKGQYAQITQMGRLTGSPISSDKPIGVFGGHQIMSID
                     RCCGDHGEQMLAPVRALGYEYVAAPHGDRRPVSEERIYRIIGAVDNTHLTYDPPNAGP
                     STVNLGQILEIRTATPFTVRSEGDNQPFLMFTYMSGAGEQGEGGWGDSDFVRMVPPKQ
                     YMTHYVFFTDPTYPFTVLTVVRQKKNGAFAPVKLACMGEVSGFQPVGSAGEYEIAHVK
                     IVDHFNSMGGCNNGVNTMDSTAPFGVWVWGWGSEDTTTGWVSYGYPAGEGVLPINDVI
                     IL"
     gene            complement(9228..10151)
                     /locus_tag="VMZ53_32480"
     CDS             complement(9228..10151)
                     /locus_tag="VMZ53_32480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33280.1"
                     /translation="MLGETVLDRYVVEEELGRGAMGTVYRGRHTKLKRPVAIKVMHDH
                     LTGEPTLMERFRREARVAGKLDHANVVSVLDIGETNDGRPVMVMEYAAGRSLKDVMVG
                     PLPVKRMLALLVQLLRGLDHAHSMGLVHRDLKPDNVILQGDLGDEVVRIVDFGIAVLR
                     GREEVDGGKLTGTGMIVGTPQYMAPEQAKCEPIDQRADLYALGVIMYEMIGGRTPFDG
                     TAMEVAVMKIDTDPPPISSRVPGVTVDRVLDAFMRRLLARAPVARFSTAHEALQVLEL
                     YQEDRLAAAAELGVIDVEQALAVISLPEPPR"
     gene            10177..11361
                     /locus_tag="VMZ53_32485"
     CDS             10177..11361
                     /locus_tag="VMZ53_32485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33281.1"
                     /translation="MRRLAVVAFVAGCDFTFNLDHLKQPPIDGQPDTPDGEIIYMDAP
                     GCFGKNGPGVAGLAQVCLTEAPPPSWTPNGEVHTGGTTNDCSMLQMQSGTSTELCIIA
                     ARNITLGGFLPTSGTRPLVLLATDTFTLLDTGGISVDSRRVAGRNGPGANESSCPGAP
                     AGTASTTGSSGGAGGSFGGRGGNGGKGTGSAQGATATAAITGLAVVRGGCRGGLGGGP
                     VPSGASGGAIYIIAGKRVEIAGAIDASGEGGGGGDIALAMGLGGDGGGGGGSGGLIGI
                     DAPMIHIASSAKLIANGGGGGGGGDGAVGGSASGGAGGEADLSAPPTFPARGGTGGTA
                     SGTGGRGASGTAGPDPGQPAGTNVSGSGGGGGGGGAGIIRFYGPTSSVVNESLSVSPP
                     PS"
     gene            complement(11469..11654)
                     /locus_tag="VMZ53_32490"
     CDS             complement(11469..11654)
                     /locus_tag="VMZ53_32490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33282.1"
                     /translation="MGWLPSLELSTSEYAKIEAGCGDAEQCQLELDFKVRELDVSGDM
                     PTSMRFSEVKVIGAHAT"
     gene            complement(11989..13242)
                     /locus_tag="VMZ53_32495"
     CDS             complement(11989..13242)
                     /locus_tag="VMZ53_32495"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HUS33283.1"
                     /translation="MKKKHFGYLAGASLIAGAITASASSHREAPMIAEDQFVDNTDVY
                     AFISPSDSNKLVMVADYVPLLLPQSGPNFYRFSEDAFYDINIDNDGDAKTDITYRYEF
                     DTSTKNGNTFLYNVGPVDSITSANLNVTQTYTLWKIAGNKKTKVMENVPVAPWNVGKR
                     SFPNYEQVALQAIKSSAGTSSFAGPRDEPFFVDLHVFDLLGVGGSPTTDGVNVMSLVL
                     EVPITDIAAGGARPTSTTDKTSLVGIHATASRPQIRILRKGRDADDYGKMIQVSRLGW
                     PLVNEVIVPLKDKDKYNRSLPKDDVANFGAYILDPEVPKLLNLVLNAGCPATPSGGRT
                     DIVGLLAPNGTVPADLLRINIAQGQTNAQSHFPNGRALADDVTDTLLTVACNNGGAIG
                     DGVNRNDKDFTTTFPYLASPHSGNP"
     gene            complement(13256..14041)
                     /locus_tag="VMZ53_32500"
     CDS             complement(13256..14041)
                     /locus_tag="VMZ53_32500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33284.1"
                     /translation="MDAVASLDGKEFYFTAFDDAKQPALFKVASTAGATPEVLAAGDP
                     LESPHGLVLSCDGKTLFAADIGGDAGAIYSIPTAGGTLSDLAPTGMLRPAALAMGPDC
                     KTLFATGRTSDWMPALFKMSITGGEAMPVWIGEPLASPTGLHVDEAGVAWVMDHRAAG
                     ADGEGVLFAIPSDGSAATEVVSDLKMGTLGGVSLTAGGGVAVMPTLDADGHGQLTSVE
                     IASGEKQQLATPDIIDPAGLRTARRAAVFAVVDSEGGAIYRAE"
     gene            14271..15539
                     /locus_tag="VMZ53_32505"
     CDS             14271..15539
                     /locus_tag="VMZ53_32505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33285.1"
                     /translation="MAKVEADIEVKSFGYDGDGSGRKLLFPHATTHKIEGRSGATTYG
                     AADAAFEGLEGRLDTLRWTAESGSAGGAWLRDDAGRFDVSVGRAEFPNGLRLTRAADY
                     GIELISPHVTFSELKLTVKGPFGRSKPVETPPEISTNGTPQRQEKLRFLDSLSGRINT
                     TVKVKLDLPVIGARSLDQQLRVPIQEGSLDFRALDESLDWLEGTFLDIKHEDQRLKLQ
                     WKVPIVGAGRDLISWSLDTDASTLASFGRVPLRALADFRFGSGKVSPTESERSRKGGV
                     LRSFTLDKIDVALSLLAPRHLDIGNGMIMFGGEDQPGMVDLKVIGAIHNKGPGSIKGA
                     IGSIDTTIKDLELGPTMLSCDRLHFDGLDDLEVTFDGFSPTSLTMIIHRVTASNFAMK
                     VGSRPAAAPTPTPSARSSSPAVPVDRTPRQ"
     gene            complement(15478..16869)
                     /gene="fumC"
                     /locus_tag="VMZ53_32510"
     CDS             complement(15478..16869)
                     /gene="fumC"
                     /locus_tag="VMZ53_32510"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HUS33286.1"
                     /translation="MGTRTESDSFGPIEVDDEHYWGAQTQRSLEHFAIGSETLPLSLI
                     YALATIKKSAAIVNRDLGKLDAKIAEQIVDAADEVLDGELDEEFPLSVWQTGSGTQTN
                     MNVNEVIASRANEKVNNKRGGKSPVHPNDHVNMSQSTNDAFPTAMHIAAATRIEDHLL
                     PSIGTLRQTLDGKAKAFADIVKIGRTHLQDATPLTLGQEISGWVNQLDHGSAAIRNAL
                     GPVYELALGGTAVGTGLNSHPEYATRTAQQIAMVTGKPFITAPNKFQALASHDALVGL
                     SGALKVLACSLTKIANDIRWLASGPRSGIGELTIPENEPGSSIMPGKVNPTQCEAMTM
                     VCAQVMGNDVSIGIAGSAGNFELNVYKPLIAYAVLQSIELLADACESFDEHCARGIEP
                     NRSEIARKVASSLMLVTALAPHIGYDKAAKVAKKAHADGTTLREAALALGFVTAEEFD
                     RLVRPETMTGPTA"
     gene            complement(16888..18471)
                     /locus_tag="VMZ53_32515"
     CDS             complement(16888..18471)
                     /locus_tag="VMZ53_32515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33287.1"
                     /translation="MRLAALFLLTGCQYMIGIESTKLQPDAPPVDVDSAIDAPPPPPV
                     NVGDGHDGPLTVSGVTFTDDLRVSLTAEVGAGSTLLKVTDVTNFKVDDEIVVMQMAGD
                     NAGQYETTHVASIMVGQLIVKPALASTYAIGAAGATQVIRVPNWSDVTILSTGRLTAH
                     PWNGTTGGVVMFRAKGTLDIQAGGVLDADALGADAGTPGDGALHGTGGPPGGGGMAAA
                     CFSLCATTAANGGGAGNGGVGLSAFGGPGGQNAKCVGGPGGRGGQHGATGASGASGNP
                     GAGIGGGIADGGAGANMSINILHPVLGAGGGGGRGGDGGDGAGGGGGGGGSVGTADGG
                     PGMQGQNGGDGGDGGAGGRGAGIVIVNATTIKIAGLVSARGEDGDPGITGTDGGKGGT
                     GGFGGGGGCNGGNTSGGRGAGGDGGESGSGGGGGGGGAGGSVELAAFDITAIDGMARA
                     TGGAGGTPGMPGAAGLAGDGYDSPSDPDDGALPGVQGSAGAAGASGGSGFFYLRYVET
                     CAGCSLGDPAATVIHIQPR"
     gene            complement(18468..19436)
                     /locus_tag="VMZ53_32520"
     CDS             complement(18468..19436)
                     /locus_tag="VMZ53_32520"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HUS33288.1"
                     /translation="MKALIPVGAGLVLAFAPMKASAQPTDSVAAAEQLFEQARALFDQ
                     GNFAEACPRFGASYKLDPALGTLLNMATCYEMLGHIASAWGHYREVVVHAQKVGDQQR
                     IDIARARIAALEPRLPKLTIKAPNVPLASLIVTRDDAPLDIAVLGASIFVDPGQHAIS
                     ASAPGYKPFTTTITIKEGEAQEVQLALEAAPNQPAPQTGGITTIIREESDPGKTRRLF
                     ALTLGGAGAVALVTGVSFGFAARDSWQSAFDDGLCNRTTLECTPEGQDLTRTARTRAL
                     VANIVGGAGIAMLAGGAILYLTAPTRADVSVTPTNGGAAVSFGGHW"
     gene            complement(19496..20947)
                     /locus_tag="VMZ53_32525"
     CDS             complement(19496..20947)
                     /locus_tag="VMZ53_32525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33289.1"
                     /translation="MQSEADLEIGTKVGEYVIDGKLGEGGFGTVFAATHPMIGKRAAV
                     KVLSRKYSSDPEVVSRFAAEARAVNQIKHRNIIDIFAFGQLEDGRSYYVMELLEGQPL
                     DKILDERGGLPVGEAVPILRELARALDAAHAKGIAHRDLKPENIFLVHDDDGIPHPKL
                     LDFGIAKLLGDASQASMHRTRTGSPIGTPYYMSPEQCRGQQNVDHRTDIYSFGCVAYQ
                     LLTGDKPFAGDNHLEIMMKQTLEEPIAPSERVAQNPVLLPLPPAVDDAIAWMMKKEPA
                     ERPPSLAEAIAALEDAAGLEPIPRASDPSIRTGPLRLTPTPGRLTPLPKNRTPAHGTT
                     ALAQTMAASEEPTAARAVTGGGVAPAKKSRWPLIAGALVLVIAAMAGVIVVQSSDEKS
                     SSKAIAEPAPPAAPVPSPAPAAAPAPAAAPAPTPAVVPDVEKPVVVEPETKAEPPKVT
                     VKKPKPVVKKPAMPPAAQGSAAPKPVYDPYARH"
     gene            complement(21046..21579)
                     /locus_tag="VMZ53_32530"
     CDS             complement(21046..21579)
                     /locus_tag="VMZ53_32530"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUS33290.1"
                     /translation="MKIITPDREQLARFHAGIYLDAFAAHREPLESWLSPRGYAFHAR
                     LVLDGDDIVGGITYELYPKSRCGLVTYMVVAPRARGRGLGRMLFMSAARELYAAGAKL
                     VLGEVHRSAPERLARFDRWGARVVDVAYVQPSLGPGLERDDGLCLIVLPPVPRVVEEG
                     VVKAFIAELYEVTEGAP"
     gene            complement(21576..>24593)
                     /locus_tag="VMZ53_32535"
     CDS             complement(21576..>24593)
                     /locus_tag="VMZ53_32535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS33291.1"
                     /translation="GQIDKAVKEYLRIVQEDPKDVRGWLKIGDLYAKKGAKQDATDTY
                     LKVARFYHDQGFFLKAVAVYKQILKLDPRLVDVILKLAELYRQLGLMSDAMQHYESVA
                     AHFHREGNTKEALDTVKKLVDLDPENIATRIKLAELYSKEGLVEEAVLQFNMACEQLR
                     RQGRQDDFVKVAERLLWHKPDNHALNRELAGLYLRRNDPRRALQKLQACFKADPRDVE
                     TLGLLALAFQALDQKAKTVSVLKELARIHVENKHRDKAGEVYRKILEIVPNDTDAMQY
                     LGVGAQAAAPPPQATPTPTPQPPPMPPQRNTPPPPPFMQPRGQTPLPSAVRAASEAAK
                     FNITSDLPSLGAQNAGNLPRNMTGSMPLVDESSLSGVDFALPEYDDADFSADMEPPPD
                     PRRASAAGERHAEEISKILAETDVYVKYGLHQKAVDHLRRVFTLDEQNVEAHERLKDI
                     FISQGREDEAVQELLKLAELIAPTDPDRAEQYLQDLLAMNGTHTGAFDLARRFRLRVA
                     RMSSVSAEVEYSGGGVHEIDPDLEDLDLNAAPPSAMGIARGGHRDSIDEFDPDELLGR
                     NIAHGSPAPGRVPEPDQYGQGYDQDFGTGAHSTRQVSPEEVQRMASGMGEYEDEGGVL
                     VDTPPRGNWDEQNYAATRFESPVYPDPAQGIDDQLDDQALGEDIDQEVAAELGGDLPF
                     DPDDARAFDAEAKGGTDANYVNGFEETDMPPTAAYDDVNDPLAVSGSYDPYGTEAGQP
                     APGYESTTMGGPVVDDVATPVGGARAESLVEDDLEEVDFYVSQGMYAEATESLRNLWE
                     RYPNHPLLIAKHREIESLQGSTSAEPELTGGTDALEIDEIEEVNADDLEEVDERGNRP
                     AASKRKPTVMLEKPLDESDAETHYDLGLAYKEMGLFDDAVKAFEKVLRAPGREIQCRV
                     MIGVCFREQGNPNEAIQHFKQGLHANGSDRERLSLYYEIGVTYEAMGDYGEALYYFEA
                     VVKRDPHFADAAQRAESLRMQGGQATHPPDDDL"
BASE COUNT         4096 a         8094 c         8000 g         4403 t
ORIGIN      
        1 cccgcagggc caatatccgc agggccaata tccgcagggg cagtacccgc aggggcagta
       61 cccgcagggc caatatccgc agggccagca gccgtatccg tatccgcaac agccgtatcc
      121 gtatccgcaa caaccgtatc cgtatccaca gcagccgtat ccgtacccgc cgcagcagca
      181 gccggcgccg cagcctcaac catctcccga ggagactgga ccgaagcgca accatctcga
      241 ggtcggtctg ctcaccgggt ttgcggcacc gatgaatcgt cgcaacgagc aagtgcctgc
      301 gcttgcaggc ctcagcgtct cggcgggcta cgtgttcggt cctgttggcg tgtggctgga
      361 cttcgacagc ctcgcgaata ccgacgcgac gcacggcacg ttcctgatct cgggcagctt
      421 cgcgggcaaa gccggcgaga agctctcgat cggcggccgg ttcggcctcg gctcgacgct
      481 cgtcaacttc cacgagcccg cgttcgacga cgtgcaggca tcgaacatcc gcttcgaagc
      541 gacgctcgac tacgcgctcg cggcatcgtg gtcgctgtgg gcgcggccgt tctcgatcga
      601 catcatgacg gcgagcgagc tcggcggtcc gatcacgacg ctacagatgc gcgtcggggt
      661 cgcgttcacg ttcggcgcac atcgtcgcgc agccgtcgcg gcgcaacaac cggcacctgc
      721 gccgcaaccg gcacctgcgc cgcaacccgc acctgcgccg caacctgcac ctgcacaacc
      781 ggcgcaaccg acggcgaccc aagcgaggaa cccatgagct ggcgcaatct ctccatcgcg
      841 ctcgcgatgg tgtgcctctt ccaatggtgg cgcgcgtgca accgtccagc gcctgtcgca
      901 gctgacgaac acgccgagtg cgagagcgag ccggcgcaga accgtgttgc gtcgagctcg
      961 tcggactcgc cctcgtcgag cactgcgcgc gcgaagcaca acgccggcaa tgacgacgcg
     1021 cccgacgaag cgccacccga gggaccgcgc ctcggccaga tcaagctccc cgcatggacg
     1081 ctatggctcg caccgcatcc cggcgagaac atgctcgact atcgcgatcg catggtcccg
     1141 ctcgcgcaga cgatgctgaa gcctcagcgc gatcgcgtgg cgcgcagccg cgatgatctc
     1201 gcgaagcaga tcggcctcga ctcgcagcag cgcgcgcagc tcgacggcgc ggtggccgac
     1261 gcggcgacgc agatcgaaga caagatgatg aacgcgttca tgagcggcga cttgaacccc
     1321 gcgacgttca agccgatgac tggcgtatcg ctcgcgcgcg acgtgctgga tgtcgtcgat
     1381 caagcgaacc ggcgtttcac gcaaacgctg cgcgaagacc aaaaagctac gctcgcgaag
     1441 catcccttcg atttcgcgga ctacttgctg ttttccgcac gctgggaaga tgcgctcggc
     1501 gtgaccaact aggcacggct caccgggacg attacgattc cgtagtgcgg ctcgtcgtcc
     1561 tgctccttgc gctcgccgca tgcgcggcga agcaggagtg caagctggat cccattccgc
     1621 ccggcgcgca tggcggcgcg ttcttgtgga aggtgcagcg cggcggcgac gtcgtgtggc
     1681 tgtacggaac gattcacgac tcggggctcg acgcggttcc gcgcgtagcg ctcaaggcgc
     1741 tcgaaggctc ggtgcggttc gtcagcgagc tcggcgactc cgagcccgac cgcgaggtct
     1801 tcatcaagta cgcgcgcgtc gaccatgggc ctggcatcga tacgcggctg ccgcctggcg
     1861 attggtacga cttgcgcgat gcgctgctcg gctcgatcaa agagaacgac ctgcgccgtg
     1921 ctgcgccttg gtacgcgatg acgctgctat cgacgaagat ggcgccgacg aaagagcaat
     1981 cgatggacgt gctgctcggc aagcgtgcgc gcgagctcgc gatgccggtc gagccgctcg
     2041 agacatggga cgagcagctg tcggcgctgc agacggcggt cgacatcgag gacctcaaag
     2101 atgcgattca cgcgcgccat acaatgacgt gcgacttctc ccgcatgcgg gcttcgtacg
     2161 aggcgggcga taccaagatg atggaggcgc tgctcgtggt gccgaaaacg caggcgacga
     2221 tgctcgatgc acgcaacgcg aggtggctgc cgaagatcga gggatacgtc gcccgcggcg
     2281 gtgctttcgt cgcggtgggg ctcggacatc tgctggggcc caagggcctg ccagcgctgc
     2341 tcgagaagga cggctacacc gtcgagcgga tcggtatcga gtgagctagc tttcgggaat
     2401 gctcgcgcaa gaggcagcgg aggcggcggc cggtgcgagg cgccagatcg aggtctgcgc
     2461 ccccgtgttc gcagacctcg gcaagcggct gcgcgcgaat ccgccgcgac tcgttgttac
     2521 atgcgcgcga ggaagctcgg accatgcggc cacgtatggc aagtacgtcg tcgagacggc
     2581 agcgcgtacg gtggtggcgt cggtcggccc cagcgtcgca tcgatgtatc accgagctcc
     2641 ccaggggctc gacggcgcgc tgttcattgc ggcctcgcag agcgggcgca gcccggatct
     2701 cgtcgagctg acgcaggctg cgcgcaaggc aggtgccctc gtcgtcggct tcatcaatgc
     2761 cgagggctcg ccgctcgctg aagcatgtga cgtcgtcgtg ccgctgtgtg ccggcgagga
     2821 gaaagccgtc gctgcgacga agtcgttctt gctgaccggc gtcgcgttcc tgcagctcgc
     2881 cgcacactgg accgaggata tcgcgctgca cgatgcagtg gcgcgggcgc cagatgctct
     2941 cgatgcggcg gctgcacaac catggccgct ggcccagctc gccggcgccg agagcctgtt
     3001 cgtcgtcggg cgcgggcttg gactgggccc ggcgcaggag atcgcgttga agctcaagga
     3061 gacatgccgc ctccacgccg aggcattcag cagcgccgag gttctgcatg gaccgatggc
     3121 gctcgttcgc gagggctttc ccgtcgtcgg tgtggtgcaa gacgacgaga cggccgagtc
     3181 gacgcgagag gttctgcaac acatcgcggc tctcggggcg accgtgcatc ttcacgttgc
     3241 gcacgaggct gcgcatcttg cgccgctgtg tcaggtgcag agcttctatc tcggacttcc
     3301 agcgctcgca gccgcgcgtg ggctcgacgc ggatctgcca gcgcatctgc acaaggtgac
     3361 agaaactcgc tagtggtgct ttttgacatc accgacctgg agccgcgggt cccgtcggaa
     3421 aactgagagt tgttgcagcg acgtgaatga aaaaatgaca gcgcgatgat cgcggcgatc
     3481 gcgcgtctga cttctcgcgg agttcgctcg tgcgcagtgt tcggtcatcc gggatccgga
     3541 cagacggcac gcgcgttgct gaagtgctcg gcatgcgccc gtcgatcttc gagggcgtcg
     3601 cggtagctgc gagcctctgc ctcgtgggct gcctcggcga tcctgcgtct ccaaccacga
     3661 ccacgctcac cgcgcgtgag gcgtttgacc agcgtgcgtg gccttcgctc tcgacctgtg
     3721 cgggttgcca cggcaaccag cccgcgatcg acttcctcgc gccgggaact gccgatggcg
     3781 cgtacacgac ggttttcgcg ttccagccgc ccgttatcga catcgaggtt ccgtcgtcga
     3841 gcctgctgct cacgatgggt aagcacaccg gtccagcact ctcggccgac caagcggcgg
     3901 gggtgctcga gtggctcgag gcggagcgca acgaacgtat gcccgagaca gggaacaccg
     3961 tgcgcgtcgg tcccgtatta cctacgcccg gcaccgcgat cacgctcgac ctcggaatca
     4021 acggcgcgca gctgacgctg atgccggagg cgagtgaagc gggcctgtac ttctcgcacg
     4081 tgacgatcgc gggcggcagt ggtgtgcatc tgacccatcc gctgttcgtg tcgcggccgc
     4141 cgaaaccgat tctcgatccc gtcgatcgct ttggcgcact cgacgcgaag gtcgccgccg
     4201 gtgggcagct cgagctcgga ccggtgtggt tcctcgactt caccactacc gactacctgt
     4261 cgatccactt ctcgaccctg gaggctccgc aatgaagcgc tacttcttgc tcgccgcgct
     4321 ggtctcgcct ctcgcgggct gcgcggctga gcccggccct agtgggcccc cgcacgatga
     4381 atgggatgac aagctcgccg atcgcgtcgt cgattatggc gccgccctgc gcatcgcatc
     4441 gctgcggctg accggcgagc tgccgacgct ggcggacatc gatgcgatcg cgaatgcgcc
     4501 ggatgaagcg agtcagaagg ccgcgtacga atcgacgatc caacgttaca tcgcgagccc
     4561 gcgcttcgcg cgtcagatgt ttcggttctc gcaggacatg cttcgcctcg gcgacgcgcc
     4621 cgagaccgac accgcgcctg cgttcctcgc gcagctcatc gtcgagaatc gtagctacct
     4681 cgacgtgctc accgccacga ctggcacgtg cccgacgttc gatgtgagga cgggcgtgtt
     4741 cactgccggc aactgcaaca acggcacgca ggcagctggc ctcctcacgc atcccggaat
     4801 gctcgccgcc aacttctcga acctcgcgtt tcgccgcgtg cggtgggtgc aagagacgtt
     4861 cgcctgcacg aagttcccgg cggagatcgc gaagacgcca accgacgtcg gaggcgcgac
     4921 gccatacacc ggcacgtttc cgttcgacag catctcgggc actccgacgg gccgcgtgaa
     4981 cttcaaggac acgtcgtccg tgatctgcgc gaactgtcac tcgaacctga accacctcgc
     5041 gccgctgttc gcgcactacg acgagaccgg tcagttccag accggctttg cggtgcccgt
     5101 gccgctgccg aacaaccctc tcgtcgcgat gagcgactac ttgcccgttg gcgagccaac
     5161 cgcctggcgc ttcggcacgc ccgtgagcga catgcgctcg ctcggcacgg cgatggcggc
     5221 cgaccctgcg attgccgagt gtgcgattgc ccgggtgtgg aactgggcgc tcggccggcc
     5281 cgacatcgtc gacgcgagcg cgaaggtccc gagcaccacg atcgaatcac aggtcgccga
     5341 cttcaaagcc ggcggctacc acctgcgcga tgcgatgtat cgcgtgttca ccagcgacga
     5401 cttcgtccgg ttctgaggag gccatcatgc gttacgcgct tcttctcctg attgccggct
     5461 gtacgaccga gatcgtcgac tcgacgacga cggggcacgc cgaggatccg tatacgtcgc
     5521 tcgagcgtga gcagcgcgag ggtccgcctc gctacacgtc gcgactccac agctgcgcaa
     5581 agatgcgcgt cgcgacgctg ggcaacgtgc ttgcctcacg aggtgtcgat ctcgcggcga
     5641 cgccggagct gtcggcagga cgcatctatc gcaccagcac gatcgcactc ggcgcggcga
     5701 actacgcggc acgcacacgc gagaacgtcg agctcggcgt cgcgaccgcg tcgaagatct
     5761 tcgacatcta tgtgcaggcc gcgcccgaga tcatcgcggc aatgccgtcg cgaccggagt
     5821 gccagcgcaa cggtcagccc gcgcagctct tcgacagcag caatcgctgc gtcgagtccg
     5881 gcttctcgtg cctgatcggg acgcgagcga cgccggagca tctggcggtc tgtaacgaaa
     5941 ccgtcaaacg cgccgccgac cctgaatccg gcaaacgtct cgctgtcgcg cttctcgcgg
     6001 ccgcggccca cacgtgtgag taggaggtct tatgactcgc gatcgtgatc gtgacatcac
     6061 ccgccgcgct ctcatccagt ggggcgttgc cgctggcgcc gctctcggcg tttcgaagtc
     6121 gcggctcgcc gagatcttcg agcgcacggg cgacaagcag ctcgcacatg ccgcatcgtc
     6181 ggcaccgacg aagcgctccg tgcacattcg cgccggcaat ggcggtctcg cgtggttcac
     6241 gctgctctgg ccgcacaacg atatcgccgc cgctgcgccg aacaatccgg tgacgacgtg
     6301 gccgcacgca cccgcggtca cacgcaagct cacgggcacc ggcggccagc tgacggtggg
     6361 tcccaacacg ccgttcgtcg ggctcgaagg tgataccgcg acggtgaccg ccttcatggc
     6421 cggcaacaac gaagcgcacg tgcagaatcc aaactcgatc gcacgctcgc tcaacggcaa
     6481 ctcgatgttc gcaatcgctt cggtgctgca gcaggcgagc ccgacggtga ttcccgtcat
     6541 caccgtcgac gacgtcgcgc tcgggaccgc gcccggttcg ccgcgtgctg ctgtcgtgcc
     6601 gaccggcctc gacattgtcg gcctgttcaa cagcgcggca tcgcgcgccg gcgggctgct
     6661 cgagcgtgcg aagtacgcgg gccatgccga tctgtatcgc tcgcacttcg agaccctcgc
     6721 gcagctcaac cgcgcatcgg gccgctcgac gacccgcgat tcgtatgcga cggcgcgcag
     6781 cgctgcaggc ttcctcggcc agaacctcgc cgcgaagctc gccgttcaac cggccgacga
     6841 gatcgcgtac ggaatcaaga atgccgacgg cacgccgagc accatgcgcc aagaggtcgt
     6901 cgcgattgct cgcacgttga tcgtgaccgc gaaggcattc cagcttcacc tcacgtcgtc
     6961 ggtcgtgctt ccgggcgtgc gcgatgatcc gcacgacgcg tttacgaata cggcgaaggc
     7021 gaacaccgcg cgcgatctga agacggtgct cgatgccttc atgcgcgatc tcaagaaccg
     7081 taacgacgac gcgaccggcg cgccgctctc cgaggacatc gtgatcacga tcgaaggcga
     7141 tacgccgaag acgccgctcg atcgcacgaa ctggctcgat gacacgccgg ggaactcgaa
     7201 ctggatgtac gtctactcag gcggcctcat caagaccggc tggctcggcg gcatcgatcg
     7261 caaccgcggc gtgaagggct tcgatccagc gaccggcgcg cccacgccgt acgacggtga
     7321 tctcggcgcc caagccgccg ttgcggccgt cgcgtacgcg atcacgaagg gcgacaagcg
     7381 acgcgtgcag gacttctcgc gcgtcgatat ctcgggaatc gtgaacgccg tctagagaat
     7441 gatgacgtcg ttgatcggca gcacgccctc gcctgccgga tagccgtagc tgacccagcc
     7501 ggtcgtcgtg tcttcgctgc cccagcccca cacccaaacg ccgaacggcg cggtcgagtc
     7561 catcgtgttc acgccgttgt tgcagccgcc catgctgttg aagtgatcga cgatcttcac
     7621 gtgcgcgatc tcgtactcgc cggcgctgcc gacgggctgg aagccgctga cctcacccat
     7681 gcacgcgagc ttcacgggcg cgaacgctcc gttcttcttc tggcgcacga ccgtgagcac
     7741 ggtgaacggg tacgtcggat cggtgaagaa cacgtagtgc gtcatgtact gcttcggcgg
     7801 caccatgcga acgaagtcgg aatcgcccca gccgccttcg ccctgctcac cggcgccgct
     7861 catgtacgtg aacatcaaga acggctggtt gtcgccctcg ctgcgcaccg tgaacggcgt
     7921 cgccgtccgg atctcgagaa tctggccgag gttgaccgtc gacggtccgg cgttcggcgg
     7981 gtcgtacgtc aggtgcgtgt tatcgaccgc gccgatgatg cgatagatgc gttcttcgct
     8041 gacgggacgc cgatcgccgt gcggcgcagc gacgtactcg tagccgagcg cgcgaaccgg
     8101 cgcgagcatc tgctcgccgt gatcgccaca gcagcgatcg atcgacatga tctgatggcc
     8161 gccgaagacg ccgatcggtt tgtccgacga gatcgggctg ccggtgagac gccccatctg
     8221 cgtgatctgc gcgtactgac ctttgttgag ctggaacgac atcgactggc ccatcgtcgc
     8281 cgggggaacg ccgttgccgc ccgtcacgtt cgacatcggg cgcatcgtga cggtcgtgcc
     8341 gtcttccttc gcgacgatgt tcatcgacgg tccctgcgcg atctggatcg gcggcgcgga
     8401 gccatcgagc gtgtcgtacg cggtgacgcc cacgtagttg ttgccccacg cgctcgtcgg
     8461 gagaaggagc gaagcgcccg tcgctgcggc cttgccaccg ccgaacggca gcatctgata
     8521 cgcaacgacc gggaggtccg tcacgatgtg gaatgccttg ccgatgccgt tgcccgcgat
     8581 ctgcgcgtcg gtgccgattg ccgcgggcac cgggcacgtc acgttgccca tgagcgggct
     8641 gcccgcgggt gcgtacgcga gaaagaggat cgcgacttgc ccgggcgcaa ggcccgtcga
     8701 cggatcgtag ttcgtgtacg tcagcgacgt gcccgagcct tgcggcagct ttgcgaactt
     8761 cgagagatcg atgccctggc caccccagtc gacatcgatg tgagcctgac cacgcgacgt
     8821 gttcgcgacg aacaccgtga agcacgcatc gcgcggcgga ccatctgcag cgtccatgtc
     8881 gacggcgtag taatcgcagc caaccgatgc gtggttgttc tctgccgccg cgcatccatt
     8941 catgcactcg ccgttcgagc acaattgatc gggcgcgcag gccttcgcgg ttccgtcgca
     9001 cgagatgacg gcggtcttgt catcgctgca acgcgggcac gtatcgttcc cgtcgtcgtc
     9061 gtcatcaccg ttgccgttac gaccacccgg accacacgcc gcgatcatca acgccagcag
     9121 tacccacagc tgcttcatgc gtgtctagta ctgagaacga agccaattcg aaagagaaaa
     9181 ctgagcacgt tcagcagttt gcacgtactg cgcaccgacg atcgcaatta gcgcggcggt
     9241 tctgggagcg agatcacggc aagtgcctgc tcgacgtcga tgacaccgag ctcggcggcg
     9301 gccgcaagcc gatcctcctg gtagagctcg agcacttgca gggcctcgtg cgccgtcgaa
     9361 aaccgcgcga ctggagcgcg cgcgagcagt ctgcgcatga atgcatcgag cacgcgatcg
     9421 acggtgacgc cgggaacgcg cgagctgatc ggtggcggat ctgtgtcgat cttcatcacc
     9481 gcaacttcca tcgcggtgcc gtcgaacggc gtccttccgc cgatcatctc gtacatgatc
     9541 acgccgagag cgtagagatc cgcgcgctga tcgatcggct cgcacttcgc ttgctcgggc
     9601 gccatgtact gcggcgtgcc gacgatcatg ccggtgccgg tcagcttgcc gccatcgact
     9661 tcttcgcggc cacgcaggac ggcgatcccg aagtcgacga tgcgcacgac ttcatcgccg
     9721 aggtcgcctt gcaggatcac gttgtcgggc ttgagatcgc gatggacgag gcccatcgag
     9781 tgcgcgtgat cgagaccgcg caacagctgc acgagcaacg cgagcatgcg cttcactgga
     9841 agcggcccca ccatcacatc cttgagactg cggcccgctg cgtactccat caccatgacg
     9901 gggcgaccat cgttggtctc gccgatgtcg agcaccgaga cgacgtttgc gtgatcgagc
     9961 ttgcccgcga cgcgcgcttc acgccgaaac cgctccatga gcgtcggctc gcccgtcaag
    10021 tggtcatgca tgaccttgat cgcgaccggc cgcttcagct tcgtgtgacg gcctcgatac
    10081 acggtgccca tcgcacctct gcccagctct tcctcgacga cgtaacggtc gagaacggtt
    10141 tcgccgagca cgcaccaatt ctattacaat gtcgtgatgc gccggctggc tgtagttgca
    10201 ttcgtcgccg gctgcgactt cacgttcaac ctcgatcacc tgaagcaacc gccgatcgat
    10261 ggtcaacccg acacacccga tggcgagatc atctacatgg atgcgccggg ctgcttcggc
    10321 aagaacgggc ctggagtagc ggggctcgca caagtgtgcc tgaccgaggc accgccgccg
    10381 tcgtggacgc ccaacggcga ggttcacacg ggcggcacga cgaacgattg ctcgatgctg
    10441 caaatgcaga gcggcacgag cacggagctc tgcatcatcg cggcgcgaaa catcacgctc
    10501 ggtggatttc tgccgacgag tggcactcgc cctctcgtat tgctcgcaac cgacacgttc
    10561 actctgctcg atacgggcgg tatcagcgtc gatagccgtc gtgtcgctgg acgaaacggt
    10621 cctggcgcga atgagagctc gtgtccgggt gcgcccgcag gaaccgcgtc gaccacgggc
    10681 agcagtggag gcgccggcgg cagcttcggc ggcaggggtg gaaacggcgg caagggtacg
    10741 ggcagcgcgc aaggcgcaac agcgacggca gcgatcacgg ggctcgcggt cgtgcgtgga
    10801 gggtgcagag gcggactcgg tggtggtccc gtcccgagcg gagcgtccgg cggcgccatc
    10861 tacatcatcg ccggcaagcg cgtcgagatc gccggtgcga tcgatgcctc cggcgaaggt
    10921 ggcggcggtg gcgacatcgc gctcgcgatg ggccttggcg gcgatggtgg tggcggtgga
    10981 ggctcgggag gcctcatcgg gatcgacgcc ccgatgatcc acatcgcctc gagcgcgaag
    11041 ctgatcgcaa acggtggcgg cggtggcggt ggtggtgatg gcgccgttgg cggtagcgcc
    11101 agcggaggcg cggggggcga agcagatcta tcggcgccgc cgacatttcc tgcgcgcgga
    11161 ggcaccggcg gtacagcaag cgggactgga ggcagaggcg catccggcac agcgggcccc
    11221 gatcctgggc aacctgcagg cacgaacgtc tctggcagcg gtggcggcgg aggcggagga
    11281 ggcgcgggca tcatccgctt ttacggcccg acgtcgagcg tcgtgaacga gagcctctcg
    11341 gtgtcgccac cgccctcgtg aaaaaaatga gaggacgtaa cctttcggcc acgtcctctc
    11401 tgcgtctcgt tcgcccctca gatgttacct gtttgacccg atgatcaagg tcacagcaag
    11461 acgcgtgctc aagtcgcgtg agcgccgatc actttgacct cagagaatcg catcgacgtc
    11521 ggcatatcac ccgagacgtc gagctcgcgc accttgaagt cgagctccag ctgacactgc
    11581 tctgcgtcgc cgcagcccgc ctcgattttc gcgtactcgc tcgtggagag ctcgaggctt
    11641 ggtagccagc ccacctgcgc cggacgctgg atcggaaacg cagcgacatt gcacgcagat
    11701 gcagagcggc acagcacgcg ttggcgagtg acagtccagt gcagtcgctg tccttgccat
    11761 cggtctttca cctcgcgcca tgtgccacga cgctccgctt gctccagttc ctgagcgaga
    11821 tctgcttgcg tcgtagcggt cagaacgggg tgcttcgcga catcgcgctc ggaaggagca
    11881 ctgcgctggt cgttacgcga acatgcaacg gtaaggctcg cgaccattgc gatcagaagt
    11941 ggtgtgcgaa tcatcatcgg cgtgctcctt ccgtgtggca ggtggctctc agggattgcc
    12001 cgaatgcggc gaggccaggt acgggaacgt cgtcgtaaag tctttgtcgt tacggttcac
    12061 gccgtcgccg atcgctccgc cgttgttgca ggcgacggtg agcagcgtgt cggtgacgtc
    12121 atcggcgagc gcgcggccgt tcggaaagtg cgactgcgcg ttcgtctgac cctgggcgat
    12181 gttgatgcgc aggaggtcag cggggaccgt gccgttcggc gcgagcagac cgacgatgtc
    12241 cgtgcggcca cccgacggcg ttgcaggaca acccgcgttg agcacgaggt tgagcagctt
    12301 cggcacttca gggtcgagga tgtacgcacc gaagttcgcg acgtcgtcct tcggcagcga
    12361 gcggttgtac ttgtccttgt ccttcagcgg aacgatgact tcgttgacga gcggccaacc
    12421 gaggcgcgag acctggatca tcttgccgta gtcatcggcg tcgcggcctt tgcgcaggat
    12481 gcggatctgc gggcgcgagg cggtcgcgtg aatgccgacg agcgacgtct tgtccgtcgt
    12541 cgacgtcggg cgtgcaccac ccgcggcgat atcggtgatc ggaacctcga gcacgaggga
    12601 catcacgttg acgccatcgg tcgtgggcga gccgccgaca ccaaggaggt cgaacacgtg
    12661 gagatcgacg aagaacggtt catcgcgcgg accggcgaac gagctcgtgc ccgccgacga
    12721 cttgatcgct tggagggcga cctgctcgta gttggggaac gagcgcttgc cgacgttcca
    12781 cggggcgacg gggacgttct ccatcacctt cgtcttcttg ttgccggcga tcttccacag
    12841 tgtgtacgtc tgcgtgacgt tcagattcgc gctcgtgatc gagtcgacgg gaccgacgtt
    12901 gtagagaaac gtgttgccgt tcttcgtcga agtgtcgaac tcgtagcgat acgtgatgtc
    12961 cgtcttcgca tcgccatcgt tgtcgatgtt gatgtcgtag aacgcgtctt ccgagaaccg
    13021 gtagaagttc gggcccgact gcggaagcag cagcgggacg tagtccgcga ccatgacgag
    13081 cttgttcgaa tcgctcggcg agatgaacgc gtagacgtca gtgttatcga cgaattgatc
    13141 ttcggcgatc atcggagcct cgcggtgaga cgaggcggat gcagtgatgg caccagcgat
    13201 cagcgatgcg ccggcgagat atccgaaatg ctttttcttc atgagctggt cccccttatt
    13261 cggcgcggta gatcgcgcca ccttctgaat cgacgactgc aaacacggcc gcccggcgcg
    13321 cggttcgcag acctgctgga tcgatgatgt cgggcgtggc gagctgctgc ttctcgccgc
    13381 tggcgatctc gacggaggtc agctgaccgt gaccgtcggc atcgagcgtt ggcatcaccg
    13441 cgacgccgcc cccggcggtt agcgagacgc ctccgagcgt gcccatcttg agatcgctga
    13501 cgacttccgt cgcggcgctg ccgtcggacg gaatcgcgaa caacacacct tcaccgtcgg
    13561 cgccggctgc gcggtgatcc atcacccacg cgacgccagc ttcatcgacg tgcagaccgg
    13621 tcggcgacgc gagcggctcg ccgatccaga cgggcatcgc ttcaccgccg gtgatcgaca
    13681 tcttgaacag cgccggcatc cagtcgctcg tgcgccccgt tgcgaacagc gtcttgcagt
    13741 ccggtcccat cgcgagcgcg gccgggcgca gcatgccggt cggcgcgaga tcgctcagcg
    13801 tgccacccgc ggtcgggatc gaatagatcg cgcccgcgtc tccaccgatg tcggcggcga
    13861 acagcgtctt gccgtcgcac gacagcacga ggccatgcgg cgactcgagt ggatcgccag
    13921 cggcgagcac ttcgggcgtt gcacctgcgg tcgacgcgac cttgaacagc gcgggctgtt
    13981 tcgcatcgtc gaacgcggtg aagtagaact ccttgccgtc gagactggcg acagcatcca
    14041 tgggcgagga gaaacctcct gtcgccactc gttccactgc ttttggcgga tcactagata
    14101 gaccgtcaga cgagttgcac gcagccagcg caagaggcgc agccgacagc gtcgcaacca
    14161 ggactcgttt ggccatgtgg ccctccccct ggaatgttga aaaccgtgtc gcgtgctgta
    14221 cgggcgactc gggaatccgg atcgaaccgg ggtacattcg gatccgctgt atggcaaaag
    14281 tcgaggcgga catcgaagtt aagagcttcg gctacgacgg agacggcagc ggcagaaagc
    14341 tgctgttccc gcacgcgacg acgcacaaga tcgaaggccg atccggcgcg acaacgtacg
    14401 gtgccgcaga cgccgccttc gaaggcctcg aaggacgtct cgatacgctg cgctggaccg
    14461 cggagtccgg ctctgctggc ggtgcgtggc tgcgcgacga cgcgggacgc ttcgatgttt
    14521 cggtcggtcg cgctgaattt ccgaacggct tacgcctgac gcgcgccgcg gattacggca
    14581 tcgagctgat ctcgccgcac gtcacgttct ccgagctgaa gctgacggtg aaaggaccat
    14641 tcggtcgcag caagccggtc gagacgccgc cggagatttc gacgaatggc acgccgcagc
    14701 ggcaagagaa gctgaggttt ctcgattcgc tgtcgggccg catcaacacc acggtgaagg
    14761 tgaagctcga tttgccggtc atcggcgcgc ggtcgctcga tcagcagctg cgcgttccga
    14821 tccaagaagg cagcctcgat tttcgcgcgc tcgacgagag cctcgattgg ctcgaaggca
    14881 cgttcctcga catcaagcac gaagatcagc gtctgaagct gcagtggaag gtgccgatcg
    14941 tcggcgccgg ccgcgatctc atctcgtggt cgctcgacac cgatgcatcg acactcgcgt
    15001 cattcggtcg cgtcccgctg cgcgcgctcg cggacttccg ctttggctcc ggcaaggtct
    15061 cgccgacgga gagtgaacgc agtcgcaagg gcggcgtgct gcgctcgttc acgctcgaca
    15121 agatcgacgt ggcgctgtca ctgctcgcgc cacgccacct cgacatcggc aacggcatga
    15181 tcatgttcgg cggcgaagat cagcccggca tggtcgacct caaggtgatc ggcgcgatcc
    15241 acaacaaggg cccgggcagt atcaagggcg cgatcggcag catcgatacg acgatcaagg
    15301 atctcgagct cggcccgacg atgctgtcgt gcgaccgtct ccacttcgat ggcctcgacg
    15361 acctcgaggt tacgttcgac ggtttctcgc cgacgtcgct cacgatgatc atccatcgcg
    15421 tcaccgcgtc gaacttcgcg atgaaagtgg gctcgcgacc tgcggccgct cctacgccta
    15481 cgccgtcggc ccggtcatcg tctccggccg taccagtcga tcgaactcct cggcagtgac
    15541 aaacccgaga gcgagcgctg cttcgcgaag cgtcgtgccg tcggcgtgtg ctttcttcgc
    15601 gaccttcgcc gctttgtcgt agccgatgtg cggagcgagt gcggtgacga gcatgaggct
    15661 gctcgcgacc ttgcgcgcga tctcgctgcg attgggctcg atgccccggg cgcaatgctc
    15721 gtcgaacgat tcgcacgcat cggcgaggag ctcgatcgac tggagcactg cgtacgcgat
    15781 cagcggcttg tagacgttga gctcgaagtt gccggctgag cctgcgatgc cgatggagac
    15841 gtcgttgccc atcacctgcg cgcagaccat cgtcatcgcc tcgcactgcg tggggttcac
    15901 cttgcccggc atgatcgagc tgcccggctc gttctcggga atcgtcagct caccgatgcc
    15961 gctgcgtggg ccgctcgcga gccagcggat gtcgttcgcg atcttcgtga ggctgcacgc
    16021 gagcaccttg agcgcgcccg agagaccgac gagcgcgtcg tgcgatgcga gcgcttgaaa
    16081 cttgttcggc gcggtgatga acggcttgcc cgtgaccatc gcgatctgct gcgccgtgcg
    16141 cgtcgcgtat tcggggtgcg agttgagacc ggtgccgacc gcggttcctc cgagcgcgag
    16201 ctcgtagacg ggaccgagcg cgttgcggat cgccgcgctc ccatgatcga gctgattcac
    16261 ccagccggag atttcctggc cgagcgtgag cggcgtcgcg tcttgcaggt gcgtgcggcc
    16321 gatcttcacg atgtcggcaa acgccttcgc tttgccatcg agcgtttggc gcaaggtgcc
    16381 gatcgacggc aatagatggt cttcgatgcg cgtcgcagcg gcgatgtgca tcgccgtcgg
    16441 gaacgcatcg ttcgtggact gcgacatgtt cacgtggtcg ttcggatgga ctggcgattt
    16501 gccaccgcgc ttgttgttca ccttctcgtt cgcgcgcgac gcgatcactt cgttgacgtt
    16561 catgttcgtc tgcgtgccac tgccggtctg ccacacggag agcgggaact cttcgtcgag
    16621 ctcaccgtcg agaacttcgt cggctgcgtc gacgatctgc tcggcgatct tggcgtcgag
    16681 cttgccgaga tcgcgattga cgatcgccgc cgacttcttg atcgtcgcga gcgcgtagat
    16741 cagcgacagc ggcaatgtct cgctgccgat cgcgaagtgc tcgagcgacc gttgcgtctg
    16801 cgcgccccag taatgctcgt cgtcgacctc gatcggtccg aagctatccg attcggtgcg
    16861 cgtgcccata cagcgggctt accacgctca cctcggctgg atgtggatca cggtcgccgc
    16921 tggatctccg aggctgcagc ctgcacacgt ttcgacgtag cgcagataga agaagccgct
    16981 gccgccactc gcgccggcag ctcccgcgga gccctgaacg cccgggagcg cgccgtcgtc
    17041 cggatcgctc ggactgtcgt agccatcacc cgcgagaccc gcggctcctg gcattccagg
    17101 cgtaccacca gcgccaccag tcgcgcgcgc cataccgtcg atggcggtga tatcgaacgc
    17161 ggcgagctcg acgctgccac ccgcgcctcc tcctccgccg ccgccgccgc tgccactctc
    17221 gccgccatcg ccgcccgcgc cgcggccgcc ggacgtgtta ccgccgttgc aaccaccgcc
    17281 accgccaaag ccgccggtgc cacccttacc gccatcggtg ccggtgatgc cggggtcgcc
    17341 gtcctcgcct cgcgcagaaa cgaggcccgc gattttgatc gtcgtcgcat tcacgatcac
    17401 gatgcccgct ccgcgcccgc ccgcaccgcc gtcgccgcca tcgccgccat tctgtccctg
    17461 cataccagga ccaccatcgg ccgtgccgac cgagccaccg ccaccgccgc caccgccggc
    17521 tccatctccg ccgtctccac cgcgacctcc tccgccaccg gcaccgagca cgggatggag
    17581 aatgttgatc gacatgttcg cgcccgcgcc gccgtctgcg atgccgccgc cgatcccagc
    17641 acccgggttg ccggaagctc cgctcgcgcc ggtcgcgcca tgttgaccgc cacgaccacc
    17701 ggggccaccg acgcatttcg cgttttgtcc acccgggcct ccgaacgcgg agagcccgac
    17761 tccaccattt ccggcacctc caccgttcgc cgccgtcgtc gcgcacagcg agaagcacgc
    17821 cgccgccatg cctccaccac ctggagggcc tcccgtgcca tgcagtgcgc cgtcacctgg
    17881 tgtacccgca tccgcgccga gggcgtccgc gtcgagaaca ccgcccgctt gaatatcgag
    17941 cgttcccttc gcgcggaaca tcaccacacc gccggtcgta ccgttccacg ggtgcgcggt
    18001 gagtctgccc gtcgagagaa tcgtcacgtc gctccaattc ggaacgcgaa tgacctgcgt
    18061 cgcgcctgcg gcaccaatcg catacgtcga cgcgagcgcg ggtttgacga tgagctggcc
    18121 gaccatgatc gatgcgacgt gcgtcgtctc gtactgaccg gcgttgtcgc cagccatctg
    18181 catgacgacg atctcgtcgt cgaccttgaa gttcgtgacg tcggtgacct tgagcagcgt
    18241 cgagccagcg ccgacctcgg cggtgagcga cacgcgaagg tcgtcggtga acgtgacacc
    18301 cgagacggtg agcgggccat cgtgaccatc gccgacgttc accggcggcg gaggtggcgc
    18361 gtcgatcgcg gaatcgacgt ccacaggtgg cgcgtctggc tgcagcttcg tcgactcgat
    18421 gccgatcatg tactggcagc ccgtgagcaa gaacaacgct gcgagtctca ccagtgacct
    18481 ccaaagctga cagccgcgcc accattcgtc ggcgtgaccg agacatcggc gcgcgtcggc
    18541 gcggtcagat acagaatcgc gccgcccgcg agcatcgcaa tgcccgcgcc gccgacgatg
    18601 ttcgcgacga gcgcgcgcgt gcgagccgta cgcgtgaggt cttgcccctc gggcgtgcat
    18661 tcgagcgtcg ttcgattgca gagtccgtcg tcgaatgcac tctgccagct gtcacgtgcc
    18721 gcaaagccga agctcacgcc ggtgacgagc gcgacggcgc ctgcaccacc gagcgtcagc
    18781 gcaaacagcc gtcgcgtctt tcccggatca ctctcctcac ggatgatcgt cgtgatgcca
    18841 ccggtctgcg gcgccggttg attcggcgct gcctcgaggg cgagctgcac ttcctgcgct
    18901 tcaccctctt tgatcgtgat cgtcgtcgtg aacggcttgt agccgggtgc tgacgcgctg
    18961 atcgcgtgct gcccgggatc gacgaagatc gatgcaccaa ggacggcgat gtcgagtggt
    19021 gcgtcgtcgc gcgtgacgat gaggctcgcg agcgggacgt tcggcgcttt gatcgttagc
    19081 ttcggcaagc gaggctcgag cgctgcgatg cgcgcacgcg cgatgtcgat tcgctgctga
    19141 tcgccgacct tctgcgcgtg gacgacgacc tcacgatagt ggccccaagc gctcgcgatg
    19201 tggccgagca tctcgtagca ggtcgccatg ttgagcagcg tgccgagcgc gggatcgagc
    19261 ttgtagctcg cgccgaatcg cggacacgcc tcggcgaagt tgccttgatc gaacagcgcg
    19321 cgcgcttgct cgaagagctg ctcggcggca gcaacggagt cggtcggctg cgcgctggcc
    19381 ttcatcggcg caaacgcgag cacgagaccg gcccccaccg gaatcaacgc tttcatctcc
    19441 aaccacgata ccggtcgctg cgtgtacgca acgccgccct cacgtgtgcc ttgcgttagt
    19501 ggcgagcgta cgggtcgtac acgggcttgg gcgctgcgct gccctgagcc gcgggaggca
    19561 ttgccggttt tttcacgacg ggctttggct tcttcaccgt gaccttcggc ggctcggcct
    19621 tcgtctcggg ttcgacaacg acgggcttct cgacatcggg aacgacggcc ggtgtgggcg
    19681 cgggcgcagc cgcgggtgct ggtgcagctg cgggtgctgg tgatggaaca ggcgcagcgg
    19741 gcggagctgg ctcggcgatg gccttcgacg atgatttctc gtcggagctc tgcaccacga
    19801 tgactcccgc catcgcagcg atgacgagca cgagggctcc cgcgatgagc ggccagcgag
    19861 atttcttcgc cggcgcaaca ccgcctcccg tcacggcgcg tgcggccgtt ggctcttccg
    19921 acgcggccat cgtttgcgcg agcgcggtcg tgccatgcgc cggcgtacgg ttcttcggca
    19981 acggcgtcag gcggcccggc gtcggcgtga ggcgaagcgg gcccgtgcgg atactcggat
    20041 cgctggcgcg cgggatgggc tcgaggccgg cagcatcttc cagcgcggcg atcgcttcgg
    20101 ccagcgacgg cgggcgttcc gcgggctcct tcttcatcat ccacgcgatc gcatcgtcga
    20161 cggccggtgg cagcggcagc agcacgggat tctgcgcgac tctctctgac ggagcgatcg
    20221 gctcctcgag ggtctgcttc atcatgatct cgaggtggtt gtcacccgcg aacggcttgt
    20281 cgccggtgag cagctgatac gcgacgcagc cgaacgagta gatgtccgtg cggtgatcga
    20341 cgttctgctg gccgcgacac tgctcggggc tcatgtaata cggcgtgccg atcggcgagc
    20401 ccgtgcgcgt gcggtgcatc gatgcctgac ttgcgtcgcc gagcagcttt gcgatcccga
    20461 agtcgagcag cttcggatga ggaatgccat cgtcgtcatg aacgaggaaa atgttctcgg
    20521 gctttagatc gcgatgcgcg atgcccttgg catgcgccgc gtcgagcgca cgtgcgagct
    20581 cgcgcaagat cggaactgct tcgccgacgg ggagaccgcc gcgttcgtcg aggatcttgt
    20641 cgagcggttg gccctcgagc agctccatca cgtaatagct gcgcccatcc tcgagctgac
    20701 cgaacgcgaa aatgtcgatg atgttgcggt gcttgatctg gttcaccgcg cgcgcttctg
    20761 ctgcgaaccg cgagacgact tccggatcgc tcgagtactt gcgcgagagg accttcacgg
    20821 cagcgcgctt gccgatcatc ggatgggtcg ccgcgaacac ggtgccaaag ccgccctcgc
    20881 cgagcttgcc gtcgatgacg tactcaccga ccttcgttcc gatctcgagg tcggcctcgc
    20941 tttgcatacg cggcaacgta tccaccaaca ctcattctgc gagcagagtt gtcgtcggtc
    21001 aacgaaccta cggagagggc acctcacgta caacgagaga ccgtctcacg gagcgccttc
    21061 cgtcacttcg tacagctcgg cgatgaaggc cttcaccaca ccttcctcga ccacacgcgg
    21121 taccggcggc agcacgatca ggcaaagtcc gtcatctcgt tcgagccccg gtccgagcga
    21181 tggctgcacg taggcaacgt cgacgacgcg cgcgccccac cggtcgaagc gagcgaggcg
    21241 ctcgggagcg ctccgatgca cttcgccgag cacgagcttc gcgccggcgg cgtagagctc
    21301 gcgcgctgcc gacatgaaga gcatccgccc gagcccgcgg ccacgcgcac gtggtgcgac
    21361 gaccatgtac gtgacgagcc cgcagcggct cttcggatag agctcgtacg tgatgcctcc
    21421 gacgatgtcg tcaccgtcga gcacgagacg cgcgtgaaac gcgtagccac gcggtgagag
    21481 ccacgactcg agcggctcac ggtgcgccgc gaacgcgtcg agatagatgc ccgcgtgaaa
    21541 gcgtgccagc tgctcgcgat cgggcgtgat gatcttcaga ggtcgtcgtc aggcggatgc
    21601 gtcgcctggc cgccttgcat gcgcagcgat tccgctcgct gggccgcgtc ggcaaagtgc
    21661 gggtcgcgct tcaccacggc ctcgaagtag tacagcgctt cgccgtagtc acccatcgct
    21721 tcgtaggtga cgccgatctc gtagtagagg ctcaggcgtt cgcgatcgct gccgttggcg
    21781 tgcaggcctt gcttgaagtg ttggatcgct tcgttcgggt tgccctgctc gcggaagcac
    21841 acgccgatca tcacgcggca ttggatctcg cggcccggcg cacgcagcac cttctcgaat
    21901 gccttcacgg catcgtcgaa gagccccatc tctttgtacg cgagaccgag atcgtagtgc
    21961 gtctccgcat cgctctcgtc gagcggcttc tcgagcatga cggtcggctt gcgcttgctg
    22021 gccgccgggc gattgccgcg ctcgtcgacc tcctcgagat cgtcggcgtt gacctcctcg
    22081 atctcgtcga tctcgagtgc gtcggttccg ccggtgagct cgggctctgc gctcgtcgag
    22141 ccttgcagcg attcgatctc gcgatgcttc gcgatgagca gcggatgatt cggatagcgc
    22201 tcccagaggt tgcgcagcga ttcggtcgcc tcggcgtaca tgccttggct gacgtagaag
    22261 tcgacctctt cgagatcgtc ctcgacgagg ctctctgcgc gcgcaccgcc gacgggcgtc
    22321 gcaacatcgt cgacgaccgg gccacccatc gtcgtcgatt cgtagccggg cgcgggctgt
    22381 cccgcttcgg ttccgtacgg gtcgtagctg cctgacaccg ccagtggatc gttgacgtcg
    22441 tcataggcgg ccgtcggcgg catgtccgtc tcttcgaacc cgttgacgta gttcgcgtcc
    22501 gtgccgccct tcgcttcggc gtcgaacgcg cgtgcgtcgt cgggatcgaa tggcaaatcg
    22561 cctccgagct ccgccgcgac ttcctgatcg atgtcttcac cgagcgcttg atcgtcgagc
    22621 tggtcgtcga tgccttgcgc agggtcggga tacacggggc tctcgaagcg cgtcgcggcg
    22681 tagttctgtt cgtcccaatt gccgcgcggc ggcgtgtcga cgaggacacc gccttcatcc
    22741 tcgtactcgc ccatgccgct cgccatgcgc tggacctctt caggcgagac ctgccgcgtc
    22801 gagtgcgcgc cggtgccgaa gtcctggtcg tagccctgtc catactgatc gggctccggc
    22861 acgcgaccgg gcgccgggct gccatgcgcg atgttgcggc cgagcagctc gtcgggatcg
    22921 aactcgtcga tcgagtcgcg atgaccgccg cgcgcgatgc ccatcgccga tggaggcgcg
    22981 gcgttgagat cgagatcttc gagatcggga tcgatctcgt ggacgccacc gccggagtac
    23041 tcgacctccg ctgacacgct cgacatgcgg gcgacgcgca gtcggaagcg acgtgcgaga
    23101 tcgaacgcgc cggtatgcgt gccgttcatc gcgagcaggt cttgcagata ttgctcggcg
    23161 cgatccggat ccgtcggcgc gatcagctct gcgagcttga gcagctcttg cacggcctcg
    23221 tcttcgcgac cttgcgagat gaagatgtcc ttgaggcgct cgtgcgcttc gacgttctgc
    23281 tcgtcgagag tgaacacgcg acgcaggtga tcgacggcct tctgatgcaa gccgtacttc
    23341 acgtagacat cggtctcggc gaggatcttc gagatctctt cggcgtgacg ctcgccagca
    23401 gcggaggcgc gacgcggatc tggaggcggc tccatgtccg cggagaaatc ggcgtcgtcg
    23461 tattcgggaa gcgcgaagtc cacgcccgac aagctcgact cgtcgacgag cggcatcgag
    23521 ccggtcatgt tgcgcgggag attgccggcg ttttgcgcac cgagcgacgg cagatcgctc
    23581 gtgatgttga acttcgcggc ttcgctcgcc gcacgcaccg ccgacggaag cggcgtctgc
    23641 ccgcgcggct gcatgaacgg cggcggagga ggcgtgttgc gctgcggcgg catcgggggc
    23701 ggctgcggcg tcggtgttgg cgtcgcctgc ggtggaggcg cggctgcttg cgctcctacg
    23761 ccgaggtatt gcatcgcatc ggtgtcgttc gggacgatct cgaggatctt gcgatagacc
    23821 tcgccggctt tgtcacgatg cttgttctcg acgtgaatgc gcgcgagctc tttgagcacg
    23881 ctgacggtct tggctttttg atcgagcgct tggaacgcga gtgcgagcag gccgagcgtc
    23941 tcgacgtcgc gcggatcggc cttgaagcac gcttgcagct tctgcagcgc gcggcgcgga
    24001 tcgttgcggc gcaagtagag acctgcgagc tcgcgattga gcgcgtggtt gtcgggcttg
    24061 tgccagagca ggcgctcggc gactttgacg aagtcgtctt gacgaccctg gcggcgcagc
    24121 tgctcgcacg ccatgttgaa ctgcaacacg gcttcctcga cgagaccctc tttcgagtag
    24181 agctcggcga gcttgatgcg cgtcgcgatg ttctccggat cgagatcgac gagcttcttg
    24241 acggtgtcga gcgcttcctt cgtgttgcct tcgcgatgga aatgcgcggc gacggactcg
    24301 tagtgctgca tcgcatcgct catcaaaccg agctggcgat agagctctgc gagcttcaag
    24361 atcacgtcga cgagtcgcgg atcgagcttg aggatctgct tgtagaccgc gaccgctttc
    24421 aggaagaaac cctgatcgtg atagaagcgc gcgaccttga gatacgtgtc ggtcgcgtcc
    24481 tgcttggcgc ccttcttcgc gtagaggtcg ccgatcttga gccacccgcg gacgtccttc
    24541 ggatcctctt ggacgatccg gaggtactcc ttcaccgctt tgtcgatttg ccc
//
LOCUS       DATLBZ010000661         5314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7376233, whole genome shotgun sequence.
ACCESSION   DATLBZ010000661 DATLBZ010000000
VERSION     DATLBZ010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7376233"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /gene="glk"
                     /locus_tag="VMZ53_32540"
     CDS             <1..1034
                     /gene="glk"
                     /locus_tag="VMZ53_32540"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HUS33292.1"
                     /translation="SGRAVVRRRSRRKQAQEALVILAGDLGGTKFVLAECDDRGQIKK
                     EQIFKCADYSSPFEIIDQFLGSDTVDAAAFGVAGPVVEGTAHITNLPWKIESSALSER
                     LGGPVTLLNDLQATALGLAILPTDKFAVLQQAKVPAHATIAVIAPGTGLGEAMLVSDG
                     KHYRALPSEAGHADFAPATDEEWQLLLFLRAKYGPHVSVERVLCGNGIGDLYDFCRST
                     SSTPEPQWLAHEMRSGDRNAAISNSAIAGNDPITKKTLSMFAAILGAEAGNQALRGLA
                     TGGVVIGGGIPPKILPALQTAALLERFTAKGRFTNWMKGVALRVSLEPRAALLGAAHS
                     AVMTREHRA"
     gene            1031..2605
                     /gene="zwf"
                     /locus_tag="VMZ53_32545"
     CDS             1031..2605
                     /gene="zwf"
                     /locus_tag="VMZ53_32545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HUS33293.1"
                     /translation="MTQRSKSPTSPPSNLSGSAVSVEILGGADNTKKRRPEPTTVVIF
                     GATGDLAGRKLAPAIYNLMVDGLLPEPTVILGVARNELSPAQFAQHLQPRVQEHSRRK
                     VEPAAWDKFAGMLDYVSGDFAKDETYTALKAKLEAAKGKGTKGNRMFYLSTPPSVFPM
                     IMEKLHQHGLIERQQQKSGVPSCRVIVEKPFGRDLNSARSLNDLISGYLTESQIYRID
                     HYLGKETVQNILVLRFGNSIFEPIWNRNHIDYVEITAAEEIGIEGRGAFYEQTGVVRD
                     IIQNHLLQVMSLVTMEVPASFAPDDIRDEKSQVLRSVRPLSLNDVANFCVRGQYRSYR
                     SEPGVAEGSETPTYAAMRFMIDSWRWQGVPFYLRAGKKLAERLTEVNIHFRSVPLVLF
                     KNEAAGSVLQPAVLTLRIQPHEGISLRFVAKVPGDGISVGNVLMNMTYADAFHRSISE
                     AYERLLLDCMRGDATLYNRRDSVDRAWELIQPVLQVWEATPGVEIYEDNSRGPESADE
                     MIARSGHGWRELSKQP"
     gene            2689..3126
                     /locus_tag="VMZ53_32550"
     CDS             2689..3126
                     /locus_tag="VMZ53_32550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33294.1"
                     /translation="MKNAVLAFVALILLATPALAEPLPDAPPPEESQFGKRVLEKVTD
                     ATNGFFAHHVDRLSHEMVVIRVDPEQQQANVDVHAGLLGFGVRVASNVTVVDGTARVK
                     PRLALAVAGKSLDLRLPTIDVAATEFRGERGVEVRLPVLRRTF"
     gene            3222..4052
                     /locus_tag="VMZ53_32555"
     CDS             3222..4052
                     /locus_tag="VMZ53_32555"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HUS33295.1"
                     /translation="MRRLPVIALLALAFAACKKGQDSAATSGPAAVDKATPQARAPGS
                     SIAGNGVAQPDNRKIVRTGALRISVGTYDEARAKLDALLQEVGGYVDSTQVSRGRNAI
                     TDATIVVRLPSDKFGAIVPRLRELGEIASESTNAADITDQYVDISARLASAQALEKRL
                     IELATERNGTIDSVLAVEKELARVRGEIEGYEGRLRMWNDQIAMSTLTLTLSTRTPEI
                     VASTSFSGRVSSAFHDSVHALREVAGWFVVLFVALLPWCLILALIALVGRKAIAKLRG
                     "
     gene            4049..4420
                     /locus_tag="VMZ53_32560"
     CDS             4049..4420
                     /locus_tag="VMZ53_32560"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HUS33296.1"
                     /translation="MKHAPGFLAIVNEAKKRIRETDVPTVAERLKRGDKFHFVDVRED
                     DEWRAGRAKGAVHLGKGIIERDIETKIPNKDDEIVLYCGGGFRSALVADVLQKMGYTN
                     VASMDGGIRGWRAAGLPEDTE"
     gene            complement(4484..>5314)
                     /locus_tag="VMZ53_32565"
     CDS             complement(4484..>5314)
                     /locus_tag="VMZ53_32565"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HUS33297.1"
                     /translation="RGDRGERGDRDRDRGNRDRRDTEMSVQDSTAPVVAVAETPDATV
                     DVAAPAAAAPAASSGREDRGGKKKDKRSTEVTEATREFWETWAENKTTRPEPAAAAPA
                     KAAKPARGEDTDAADADGDSDAGEERTERKARGERSERGGRGGRERGGRSSSRDKKSD
                     KGDREEKRSTKKSDDDSSSSRTKRDTAVNGGASAAAAGDQARLFISLGKKHGVSADDL
                     RALLAGPLGGDTSRIGSVSLRDSHAHVRVPEDAVDAIIAAVHGTLHNDHNVTVERARA
                     "
BASE COUNT          919 a         1764 c         1732 g          899 t
ORIGIN      
        1 ggtccggacg tgcggtggtt cgtcgacgca gccgtcgcaa gcaagctcag gaggcgctcg
       61 tgattctcgc tggtgacctc ggtggtacga agttcgtgct cgccgaatgc gacgatcggg
      121 gacagatcaa gaaagagcag atcttcaagt gcgcggacta cagctcgccc ttcgagatca
      181 tcgaccagtt tctcggtagc gatacggtcg atgcggcggc gttcggggtc gcgggtcctg
      241 tcgtcgaggg cacggcgcac atcacgaacc tgccgtggaa gatcgagagc agcgcacttt
      301 ccgaacggct cggcggtccg gtcacgctgc tcaacgatct ccaggcgacc gcgctcgggt
      361 tggcgatcct gccgaccgac aagttcgcgg tcctccagca agcaaaggtc ccggcacacg
      421 cgacgatcgc agtgatcgcg cccggcaccg ggctcggcga ggcgatgctc gtctccgacg
      481 gcaagcacta tcgcgcgctg ccatcggaag cgggtcacgc agatttcgcg ccggcgaccg
      541 acgaggaatg gcagctgctt ctgttcctgc gcgccaagta cgggccgcac gtcagcgtcg
      601 agcgcgtgct ctgcggcaat ggcatcggcg acctctacga cttctgtcgc tcgacgagct
      661 cgacgccaga gccccagtgg ctcgcgcacg agatgcgatc aggcgaccgc aacgctgcca
      721 tctcgaacag cgcgatcgcg ggcaacgatc cgatcacgaa gaagacactg tcgatgttcg
      781 ccgcgatcct cggcgccgaa gccggcaacc aggcactgcg cggcctcgcc accggcggcg
      841 tcgtgatcgg tggcggcatc ccgccgaaga tcctgcctgc actgcaaaca gctgcgctgc
      901 tcgagcgctt caccgcaaag ggccgcttta cgaactggat gaagggcgtc gcgcttcgcg
      961 tctcactcga gccgcgcgca gcgttgctag gcgccgccca cagtgctgtg atgacgaggg
     1021 agcaccgcgc atgacgcaac gttcgaagag tccaacgtcg cctccgtcca acctctctgg
     1081 ctctgccgtc agcgtcgaga tcctcggcgg tgccgataac accaagaagc gcagacccga
     1141 gccgacgacg gtcgtgatct tcggcgcaac gggcgacctc gcaggtcgca agctcgcgcc
     1201 ggcgatctac aacctgatgg tcgatggcct gctgcccgag ccgaccgtca tcctcggcgt
     1261 cgcgcgcaac gagctctccc ccgcccagtt cgcgcagcac ctgcagccgc gtgtgcaaga
     1321 acactcgcgt cgcaaggtcg agccagccgc gtgggacaag ttcgccggca tgctcgacta
     1381 cgtctcgggc gacttcgcga aggacgagac gtacaccgcg ctaaaggcca agctcgaagc
     1441 cgcgaaaggc aagggcacca agggcaaccg gatgttctat ctgtcgacgc cgcccagcgt
     1501 cttcccgatg atcatggaga agctgcacca acacggcctc atcgagcgcc agcagcaaaa
     1561 gtccggcgtc ccgtcgtgtc gcgtgatcgt cgagaagccg ttcggccgcg acctcaattc
     1621 ggcgcgctct ctcaacgatc tcatcagcgg ttacctcacc gagtcgcaga tctatcggat
     1681 cgatcactac ctcggtaaag agacggtcca gaacatcttg gtgctgcggt tcggcaactc
     1741 gatcttcgag ccgatctgga accgcaacca catcgactac gtcgagatca ccgccgcaga
     1801 agaaatcggc atcgaaggtc gcggcgcgtt ctacgaacag acgggcgtcg ttcgcgacat
     1861 catccagaac cacctgctgc aggtgatgtc actcgtcacg atggaagtgc cggcctcgtt
     1921 cgcgcccgac gacattcgcg acgagaagtc gcaagtgctg cgctccgttc ggccgctgtc
     1981 gctcaacgac gtcgcgaact tctgcgtgcg gggccaatac cgttcgtacc gcagcgagcc
     2041 gggcgtggcc gaaggctccg agacgccgac gtacgcggcg atgcgtttca tgatcgacag
     2101 ctggcgctgg caaggcgtcc cgttctacct gcgcgccggc aagaagctcg cggagcgcct
     2161 cacggaggtg aacatccact tccgctccgt gccgctcgtg ctcttcaaga acgaagcagc
     2221 tggctccgtc ctccaacccg ccgtgctcac gcttcgcatc cagccgcacg aaggcatctc
     2281 gctgcgcttc gtagccaaag tcccaggcga cggcatcagc gtcggcaacg tgctgatgaa
     2341 catgacgtac gccgacgcgt tccaccgctc gatcagcgag gcgtacgagc gcttgctcct
     2401 cgactgcatg cgaggcgacg cgacgctcta caaccgccgc gactcggtcg accgcgcgtg
     2461 ggagctgatc cagcccgtcc ttcaagtctg ggaagcgacg ccaggtgtcg agatctacga
     2521 ggacaactcg cgcggtccag aatccgccga cgagatgatc gcccgcagtg gacacggctg
     2581 gcgcgagctc tcgaagcagc cgtagatcac gcgcaactca cgccgcggca cgtcgccctc
     2641 acgggtgacc tacagtgccc tcacgcctgg gcgggcgacg gtgaggacat gaagaacgcc
     2701 gtccttgcct ttgttgcctt gatcctgctc gccacccctg cgctcgccga gccgctgcca
     2761 gacgcgccgc cgcccgaaga gtcgcaattc ggcaagcgcg tgctcgagaa ggtcaccgat
     2821 gcgacgaatg gcttctttgc gcatcacgtc gatcgtctca gtcacgagat ggtggtgatc
     2881 cgcgtcgacc cggagcagca gcaagcgaac gtcgacgtcc acgcaggcct tctcggcttt
     2941 ggtgtgcggg tcgcgtcaaa cgtgacggtc gtcgatggca ccgcgcgcgt gaagccgcgc
     3001 ctcgcgctgg ccgtcgccgg taagtcgctg gacctgaggc tgccgacgat cgacgttgcg
     3061 gcaaccgagt tccgcggcga gcgcggcgtc gaggtgcggc tccccgtgct tcgccggacc
     3121 ttctgagcgc ttagcgcgtg tcacgaggcg gcgacaaagc atgaccattg agtgcgacga
     3181 ggctttgcgc cacgcgcgtt gcaatcggca tgtctccatt gatgcgtcgc cttcctgtca
     3241 tcgcattgct cgctcttgca ttcgccgcgt gcaagaaggg ccaagactcc gcagcgacct
     3301 ccggccccgc cgccgtcgac aaggcaacgc cacaggcgcg agcgccggga agcagcatcg
     3361 ccggcaatgg cgtagcgcag cccgacaacc ggaagatcgt acgcacgggc gcgctgcgga
     3421 tttctgtcgg cacgtacgat gaagcccgcg cgaagctcga cgcgctactc caggaggtcg
     3481 gcggctacgt cgattcgacg caagtctcgc gcggccgcaa cgcgatcacc gacgcgacga
     3541 tcgtcgtgcg cctgccatcg gacaagttcg gcgcgatcgt tccgcgcctt cgcgagctcg
     3601 gcgagatcgc cagcgagtcc acgaacgccg cggacatcac cgatcagtac gtcgacatct
     3661 ctgcgcgcct cgcttcagcg caagcgctcg agaagcgcct catcgagctc gccaccgagc
     3721 gcaacggaac gatcgactcg gtgctcgcgg tcgagaagga gcttgcacgc gtgcgcggcg
     3781 agatcgaagg ctacgagggt cggttgcgca tgtggaacga tcagatcgcg atgtcgacgc
     3841 tgaccctgac gctgtcgacc cggactcccg agatcgtggc aagcacgtca ttcagtggcc
     3901 gcgtttcgag cgcatttcac gactccgtcc acgcgttgcg cgaagtggcg gggtggtttg
     3961 tggtcctgtt cgttgcgctg ctgccgtggt gcttgattct ggcgctgatt gcattggtcg
     4021 ggcgtaaagc aatcgctaag cttcgcgggt gaagcacgcg cccggttttc tcgcgatcgt
     4081 gaacgaggct aagaagcgca tccgcgagac ggatgttccg acggtggccg agcggctgaa
     4141 gcgcggcgac aagttccact tcgtcgacgt gcgtgaggac gacgaatggc gcgccggtcg
     4201 cgccaagggt gccgtccacc tcggcaaggg catcatcgag cgcgatatcg agacaaagat
     4261 tccgaacaag gacgacgaaa tcgtcctgta ttgcggcggt ggatttcgct cggcgctcgt
     4321 cgccgatgtg ctgcagaaga tgggctacac gaatgtcgcg tcgatggatg gtggcattcg
     4381 tgggtggcgg gcggcgggac tgcccgaaga caccgagtag cgccgaaaac gaaaacggcc
     4441 cgccggtgaa aggcgagccg tgtcgatcga cgaacggggc gggttaggcg cgggcgcgct
     4501 cgaccgtcac gttgtgatcg ttgtgcagcg tgccgtgcac ggcggcgatg atcgcgtcga
     4561 ccgcgtcttc ggggacgcgc acgtgggcgt gcgagtcacg gaggctgacc gagccgatac
     4621 gcgacgtgtc gccgccgagc ggtccggcga gcagcgcgcg gaggtcatcg gcggagacgc
     4681 cgtgcttctt gccgaggctg atgaacaggc gagcctggtc accggctgcg gcagcggagg
     4741 cgccgccgtt cacggccgtg tcgcgcttgg tgcgcgacga gctgctgtcg tcgtccgact
     4801 tcttggtcga gcgcttctct tcgcgatcgc ccttgtcgga cttcttgtcg cggctgctgc
     4861 tgcggccgcc acgctcgcga ccaccgcgac cgccacgttc gctgcgttcg ccacgagctt
     4921 tacgctcggt gcgctcttca cctgcgtcgc tgtcgccgtc ggcgtcagca gcgtcggtgt
     4981 cttcaccacg cgcgggcttg gcggccttcg cgggtgcggc agcggcgggc tcggggcggg
     5041 tggtcttgtt ttcggcccac gtctcccaga actcgcgcgt cgcttcggtc acttcggtcg
     5101 agcgcttgtc cttcttcttg ccaccgcggt cttcgcggcc cgacgacgca gccggcgcgg
     5161 cagcggccgg ggcagcgacg tcaacggtcg cgtccggcgt ctcggcgacc gccacgacgg
     5221 gcgcggtcga atcctggacg ctcatttcag tatcacgacg gtcgcggttg ccacggtcgc
     5281 ggtcacgatc gccgcgctca ccacggtcgc cacg
//
LOCUS       DATLBZ010000662         8690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_11099849, whole genome shotgun sequence.
ACCESSION   DATLBZ010000662 DATLBZ010000000
VERSION     DATLBZ010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11099849"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            824..2230
                     /locus_tag="VMZ53_32570"
     CDS             824..2230
                     /locus_tag="VMZ53_32570"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HUS33298.1"
                     /translation="MSRDAMVKVEREVVAEHVRVDRALRRIAVAHAALDADEAHWLRI
                     AEQRRAWPKLGYVNGFEYLEDVFGYAPRTAAERLRVARELGDLPKLEDALRCGEMSWS
                     VARELTRVATPATLDRWMEAARGRRLRDVEQMVAGRKRGDSPDDVVDPARVKHRVVLE
                     LDGESLAIWRQMQQVVESESVSGGKLDDRGFVLALGTRVLAGDASASAANAVTTASAT
                     AATTVANENARSTTAARSKPDLRSVGARYMIHITTCRDCERGWQDGAGVRVEVDRSVI
                     DRAQCNAIVCDDEKGKRPKRAIPARVERLVLERAQHRCEVPGCRSSKHLAIHHIVFWS
                     HGGTHDPSNLIVLCDGHHRVLHDGLLTISGKAPDELRFERGGAPVVGREGHVVEKHVG
                     AAPLRAKTAVARIKKAMTAPDLHEDDPLALAQAALRQLGFNAPLAARAVDAAAAHVGT
                     NVELDVLIKEALRQCRER"
     gene            complement(2760..3047)
                     /locus_tag="VMZ53_32575"
     CDS             complement(2760..3047)
                     /locus_tag="VMZ53_32575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33299.1"
                     /translation="MKLSKWLLLVPTLWVTVYCSGSLVDVDLGAVVNWTTSMLWMALG
                     LVYIVHIWRNKSFVMTTAKRILWTLAMLGFGPIAMPLYFVFHVLPDDVAAQ"
     gene            complement(3047..3373)
                     /locus_tag="VMZ53_32580"
     CDS             complement(3047..3373)
                     /locus_tag="VMZ53_32580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33300.1"
                     /translation="MKLSRPIKLLVGAATLWLPLYMLAFIATIFAGVATHSAPDVDAF
                     EILFRVHMATMVLSMGLLAFYVVHLFKATHLASDQRIMWALVILFFGPFAMPVYFVNH
                     VWPEAL"
     gene            complement(3435..4100)
                     /locus_tag="VMZ53_32585"
     CDS             complement(3435..4100)
                     /locus_tag="VMZ53_32585"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HUS33301.1"
                     /translation="MEVRTLGQFLVGEGLLADLAKIVGDLNAHATTNAQALGKQQKLP
                     VVQCFNCDAKKMCCHSIVVARFYEGCVVAAYLRDAGRDTPELRAELRAVAEAMEATNP
                     RDWRKPCHFLDADERCTVYTARPTPCGSLYVYTPPANCSDPTAAVQAYVAHEENGIAQ
                     QLEDQFREKLSLRKKVGRRYIGVLPRMVLVALEAWDRTDFRDYLRSLDWPSDSDASRW
                     TGR"
     gene            4124..4702
                     /locus_tag="VMZ53_32590"
     CDS             4124..4702
                     /locus_tag="VMZ53_32590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUS33302.1"
                     /translation="MARLVTKTSKIMHERRTIPVAGSEPVRLACVSDTHSQPHAKTIE
                     RLRELAPTAILHGGDIGDLEVLDALAQVAPVYAVRGNIDTRAKDVPDVLTIDVGALRI
                     LLTHIAVYGPKLRGEVAQLAHREKAQLVVCGHSHVPFIGLDKGVTVFNPGSVGPRRFS
                     LPICLGSITISSAGVKLLHINCETGQPWLPPV"
     gene            4839..6086
                     /locus_tag="VMZ53_32595"
     CDS             4839..6086
                     /locus_tag="VMZ53_32595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33303.1"
                     /translation="MKRFAVLVSLLASACIGEIGGVGENPPMNDPPDEPMPAPTEVRI
                     AVHDPSGPVSGVAVVFMNADDSVAADIVTDAQGIAVAQLPMGGSVSVIRELGASPTNA
                     SAGSVYTYVGVKAGDKLDVALPTAPTGTPQTITVTLPEPDPDTGKVPVEVRTPCGSGQ
                     GIPPEVTVTLDGSCGATTDFYVYELAGDEPAAFIKRAAVAATVDLSTEMYRPALATQL
                     SVMNAPTGATVYVEKTLETDLFRPVFSTGMLAVAQDQAVEALIPDLPGVEEQLVATVS
                     YNGSAQRVGLRKPYAAAPGIVDLSTAMIIGPSAPTLATDTLTWTEVGTGMPDLVVGEL
                     KGASAHRYIVGPYAGASLRIPRLPATHGAFNVSSSDSISVALAKVSGGYDGVRAHVFA
                     GPIAPLNGSATLSVATSATDAKP"
     gene            complement(6096..6926)
                     /locus_tag="VMZ53_32600"
     CDS             complement(6096..6926)
                     /locus_tag="VMZ53_32600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HUS33304.1"
                     /translation="MKAAFVLALLALSGCAVAGDESSDFESEIKNAHMVLRTNLTGMV
                     MIDSSDAFCSGMVISDTAVLTAAHCVCTNNSVFSNSCLTTAMVTFRNDPVTGKSVPRV
                     TGNVIANPDYNPSWSGAQIEGDIAIIRIPPGSRPSWVRPFQVASSYSPPGTKLMISGY
                     GHFGSGCTGPIEILMWDDAYVDHYEDDGGIVALKDNEFCSGDSGGALLLADGTLSPKK
                     LAGVISSTTPAFGTSKAVPTWAYYGWIAANVPDLGALILTDEQKDRLKDGISNGAIDL
                     "
     gene            complement(6968..>8690)
                     /locus_tag="VMZ53_32605"
     CDS             complement(6968..>8690)
                     /locus_tag="VMZ53_32605"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HUS33305.1"
                     /translation="SKTNAERLRERRDTILRKLIAAGVFSQADATAALAEAKDAAPPT
                     KLRPTPRQASHVANWLKARSPGELRIATTLDKGTQALVEKVVALHAEELRRKNIYNGA
                     IVVADHTTQEIVAVVGNIDATDFKHGGQIGMFARPRSPGSTLKPMLYAIAIDRGVALP
                     DYLVADVPMDFGAYRPHNFDGDYLGLVTLRESLAKSLNVPFVKLLAEVGTETFVAELS
                     RMGVASARAKPGELGLSLIAGGMEVTPMELAGMYAALAEDGVYRPLALRAGAPRGKSS
                     TIYGPAAAYLTRTDLSTRDRPDFPKRRDMGIPTEIHWKTGTSFGYRDAWSVGSGPRYT
                     AVVWTGNVDNTPSHELVGSEAAGPLLFDVLEGVADRRHATMSAAPPADLIEIEVCSYS
                     GHIATDACPHKVKVKAPMHSVPTTPCPYHQMLDVDRVTKQAVLPACRLDDHDYDKQSF
                     TVLPSEVTSWLRERNRAIPNAPDFAEGCVAQTSAPTITLPTEGQIVLLIPGVPASNQQ
                     VPFQVSTSSADVTWFVDGALVGTALGNERYFWEPTIGTHQIVVTDASGRKARRKLVVK
                     MGTSQQR"
BASE COUNT         1515 a         2708 c         2964 g         1503 t
ORIGIN      
        1 tggggcgacg tggcgaacga gggttcgtgc gatgtgctcg tgcgatgtgc tcgtgcgatg
       61 tgcccacgtg aacgtgaggg ttcctgcgat ctgttcgtgc gatgtgccca agtggggcgt
      121 gcgggttcgt gcgatgtggc gacgtgagcg tgagggttcg cgcgatgcga tgggcctacg
      181 tggccgtgag gttcgcgatg tggcgatgtg gaatgagggt tcgtgcgatg tgctcgtgcg
      241 atgggcccac gtgggcgtga tgcgatgtgc tcctgcgatc tgcccacgtg ggcgtgatgc
      301 gatgtgctca tgcgatctgc ccacgtgggt gggcgtgagg ttcgcgcgat ctgtcgacgc
      361 acaagaagct tgaagctcgt cgttgggaac gaaccagcgc cgcatgctcc gaacaagctg
      421 ctcagagcgc cgggccggcg gagggcggcg cgccccggcc tttcaaacgg ccaagggcgc
      481 gcgccgcgac ttcgccgccg gcccaggcgc ggctcgctcg ctccctcgca tttctccgat
      541 cacatcgtcg tcgctgtgct gtgcccaatc ggtcgagccg gttcgcaatg agctcatagc
      601 tatgacgcga tctctgacga caatctttga caactttcgt gttgacggat ttcgtagcga
      661 aacgaaatcg ctgccgcgaa gtcgctcacg atccaacgtc tgactgcatt gcacgcgatc
      721 caacgcacgt aatcgacgcg gacgatttct cgtcgagaaa accgcaatgc cgcgcgcaga
      781 acgcaaaccg tggcgccaaa atctcgcgtg gtagtgaacg ttcatgtcac gcgatgcaat
      841 ggtgaaggtc gagcgcgagg tcgttgcgga acacgtgcgc gtagatcgcg cgttgcggcg
      901 gattgcagtt gcgcatgcgg ctctggacgc cgatgaagcg cactggctgc gcattgctga
      961 gcagcgacgc gcctggccga agctcgggta cgtgaatggc ttcgaatatc tcgaagacgt
     1021 ctttgggtat gccccgcgga cagcggccga gcgcttacgc gtcgcgcgcg agcttggcga
     1081 cctgccgaag ctcgaggacg cgttgcgatg cggcgagatg agttggtcgg ttgcgcgcga
     1141 gctcacgcgc gtggcgacac ccgcaacgct cgaccgttgg atggaagcag cgcgcgggcg
     1201 ccggctgcgt gatgtcgagc agatggtggc agggcgcaag aggggcgata gccccgacga
     1261 tgtcgtcgat cccgctcgcg taaagcatcg cgtagtactc gagctcgacg gcgagagcct
     1321 ggcgatttgg cgacagatgc agcaggtggt cgagagcgag agcgtgagcg gcggcaagct
     1381 cgatgaccgc ggcttcgtgc tcgcgctcgg cacgcgcgtg cttgccggcg atgcgagcgc
     1441 aagtgccgcg aacgcagtga ccacggctag cgcaacggcc gccacgactg tcgcgaacga
     1501 gaacgcgaga tctacgactg cggcgcggtc gaagccagac ctgcggagcg tgggcgctcg
     1561 gtacatgatc cacatcacga cgtgtcgtga ttgcgagcga ggctggcagg acggcgcggg
     1621 cgtgcgagtc gaagtcgatc ggtccgtgat cgaccgcgct cagtgcaacg cgatcgtctg
     1681 tgacgatgag aagggaaagc gaccgaagcg cgccatccct gcgcgcgtcg aacgtctcgt
     1741 gctcgagcgc gcgcaacatc gctgcgaagt gccgggttgc cgctcgtcga agcacctggc
     1801 gattcatcac atcgtgttct ggtcgcacgg cggaacgcac gatccgtcga acttgatcgt
     1861 gctctgtgat gggcaccatc gcgtcttgca cgacggactg ctgacaattt cggggaaggc
     1921 gcccgatgag ctcaggttcg aacgaggcgg cgcacccgtg gtgggccgcg aaggacatgt
     1981 ggtggaaaag cacgtcggag cggcgccgct tagagcgaag acggcagtgg cgcggataaa
     2041 gaaggcgatg acggcgccgg acctgcacga agatgatccg ctcgcgctcg cacaggccgc
     2101 tcttcggcag ctcggcttca acgcgccgct cgcggcccgc gcggtcgacg cggcggctgc
     2161 ccacgtgggc acgaacgtag agctcgatgt cttgatcaaa gaggcgctgc gacagtgccg
     2221 cgaacgatga ggcgatcgcg cccacgtggg cagaccgcag gacgaccatc ggcacacgga
     2281 gcatcgtcgt gcggacgcac aacgcagcca tgtgcgatgt gatcgtcaca ccgcaggacc
     2341 cttcgtgcgc acgcactgcg cagcgatgtg cggtgtgtgg tcgccaactt cgatctgatc
     2401 gtcacaccgc aggaaccttc gtgcgcacgc actgcgcagc agtgtgcgat gtgatcggaa
     2461 gggatgcgaa agagctggtg agccgcgcct gggccggcgg cgaagtcgcg gcgcgcgccc
     2521 cttggccgtt tgcaaggccg gggcgcgccg ctccccgccg gcccggcgct ctgagcagct
     2581 attcggaaga cggagcgtct gcttcagcgc agcaggaagc tctgctcatc gcgacatcga
     2641 tcaaggaaag gagctcacgg cgaccagacg agatcacctg cacgccgtcg tgcgcaacac
     2701 cgaacgatgc gtcatgccca cgtgggcata tcgcgccgcg cccgatacca ggccgcgcat
     2761 tactgagcag caacgtcgtc gggcagcacg tggaacacga agtacagcgg catcgcgatc
     2821 gggccgaatc cgagcatcgc gagcgtccac aggatccgct tcgcggtcgt catcacgaac
     2881 gacttgttac gccagatgtg cacgatgtag acgaggccga gcgccatcca cagcatgctc
     2941 gtggtccagt tcacgacggc gccgaggtcg acatcgacga ggctaccgct gcagtagacg
     3001 gtgacccaca gcgttgggac gagcagcagc cacttcgaga gcttcattag agcgcctccg
     3061 gccaaacgtg gttgacgaag tagacgggca tcgcgaacgg tccgaagaag aggatgacga
     3121 gcgcccacat gatgcgttgg tcactcgcga ggtgcgtcgc cttgaacaga tgcacgacgt
     3181 agaacgcaag gaggcccatg ctgagcacca tcgtcgccat gtggacgcgg aagaggatct
     3241 cgaatgcgtc gacgtccggc gccgagtgcg tggccactcc ggcaaagatg gtcgcgatga
     3301 acgcgagcat gtagagcggc agccacaacg ttgccgcacc gacgagcagc ttgatgggac
     3361 gcgagagctt cattgcccgt caaactaatg acggaaatcg actcagctag atccgattcc
     3421 gggaatacgg acgcttagcg accagtccag cgcgatgcgt cagagtcgga cggccagtcg
     3481 agcgagcgca ggtaatcgcg aaaatcggtg cggtcccacg cttcgagcgc gacgagaacc
     3541 atgcgcggca agacgccgat gtagcggcga ccgactttct tgcgcagcga tagcttctcg
     3601 cggaactgat cctcgagctg ttgcgcgatg ccgttctcct cgtgcgcgac gtacgcctgg
     3661 acagccgcgg ttggatcgct gcagttcgcg ggcggcgtgt agacgtagag cgagccgcac
     3721 ggtgtgggcc gcgccgtgta gaccgtgcag cgttcgtcgg cgtcgaggaa gtgacacggc
     3781 ttgcgccagt cgcgcgggtt cgtggcttcc atcgcttcgg cgaccgcgcg cagctcggcg
     3841 cggagctctg gcgtatcgcg gccggcatcg cgaaggtacg cggcgacgac gcagccttcg
     3901 tagaagcgcg ccacgacgat cgagtggcag cacatcttct tcgcatcgca gttgaagcat
     3961 tggacgacgg gcagcttctg ctgcttgccg agcgcctgcg cgttcgtcgt ggcgtgtgcg
     4021 ttgaggtcgc cgacgatctt tgcgagatcg gcgagcagtc cttctccgac gagaaattgc
     4081 ccgagggtgc gaacctccac cagtccatgc tacgacgttc ggcgtggcgc ggctcgtgac
     4141 gaagacgtcg aagatcatgc acgagcggcg cacgattccg gtggccggca gcgaacccgt
     4201 gcgccttgcg tgcgtttcgg atacgcactc gcagccgcac gccaagacga tcgagcggct
     4261 ccgcgagctc gcgccgaccg cgatcctgca cggcggtgac atcggcgatc tcgaagtgct
     4321 cgacgcgctc gcgcaggtgg cacctgtcta cgccgtccgc ggcaatatcg atacgcgcgc
     4381 gaaggatgtg cccgacgtgc tgacgatcga tgtcggcgcg ctgcggatct tgctcacgca
     4441 catcgccgtg tacgggccga agctgcgcgg cgaggttgca caactcgcgc accgcgagaa
     4501 ggcgcagctc gtcgtgtgcg ggcattcaca tgtgccgttc atcggcctcg ataaaggcgt
     4561 caccgtcttc aatcccggat ccgtcgggcc gcggcgcttc tcgctgccga tttgcctcgg
     4621 ctcgatcacg atctcgtcag ctggtgtgaa gctcttgcac atcaactgcg agacgggcca
     4681 gccatggctg ccgccggtct gagctagagg ctcgctccca gggggtgctc ccaggggtcg
     4741 attgactcca tcgccaagtg cgcgtgctct cagcggtgcg cgtgcgccgc ggtgaagcga
     4801 agcgctggca cggaaggtga ttgagggctg aggacaccat gaagcgattt gccgtcctcg
     4861 tatcgttgtt ggcgtcggcg tgtatcggag agatcggtgg cgtgggcgag aacccgccaa
     4921 tgaatgatcc accggacgaa ccgatgcctg ctccaacgga agtgcgcatc gcggtgcacg
     4981 atccaagcgg gcccgtcagc ggcgtcgcgg tcgtgttcat gaacgccgac gactcggtcg
     5041 ccgcggacat cgtgaccgat gcgcagggca tcgcggtcgc gcagttgccg atgggtggca
     5101 gtgtcagcgt cattcgcgag ctcggcgcgt cgccaaccaa cgcttcggcg ggttctgtct
     5161 atacgtacgt tggcgtgaag gcgggcgaca agctcgacgt tgccctcccc acggccccga
     5221 ccggaacgcc gcagacgatc acggtcacgt tgccggagcc cgacccggat acggggaagg
     5281 tcccggtgga agtccggacg ccctgcggct cgggccaagg catcccgccc gaagtcacgg
     5341 tcacgctcga tggcagctgc ggcgcgacga ccgacttcta cgtatacgag ctcgccggcg
     5401 acgagcccgc cgcattcatc aagcgcgctg cggtggcggc gaccgttgat ctctcgacgg
     5461 agatgtatcg cccagcgcta gcgacgcagc tgtccgtcat gaatgcgccg accggcgcga
     5521 cggtctacgt ggagaagacg ctcgagacgg atctgttccg tcccgtgttc tcgacgggca
     5581 tgttggccgt cgcgcaagac caagccgtcg aggccctgat cccggatctc cccggcgtcg
     5641 aggagcagct cgtcgcgacg gtctcgtaca acggcagcgc gcagcgcgtg ggccttcgca
     5701 agccgtacgc agccgcgccg ggcatcgtcg atctctcgac cgcgatgatc atcgggccga
     5761 gcgcaccgac gctcgccacg gacacgctca cctggacgga ggtcggtacc ggcatgcccg
     5821 atctcgtcgt cggtgagctc aagggcgcat cggctcatcg ctacatcgtc ggcccctatg
     5881 ccggcgcttc gctgcgaatc ccgcgcctgc ccgccacgca tggggcattc aacgtctcgt
     5941 cgagcgacag catctcggtc gccctcgcca aagtcagcgg cggctacgac ggagtgcgcg
     6001 cgcacgtgtt cgccggaccg atcgcaccgc tcaacggcag cgcgacgctg tcggtggcga
     6061 cgagtgcgac tgacgcgaag ccgtagccga cgacgtcaca ggtcgatcgc gccgttggag
     6121 atcccatctt tgagccgatc cttctgctcg tccgtgagga tcagcgctcc cagatcgggc
     6181 acgttcgctg cgatccaccc gtagtacgcc cacgtcggta ccgccttgct cgtgccgaac
     6241 gcgggtgtcg tcgacgagat cacgccggcg agcttcttcg gggacaacgt gccgtctgcg
     6301 agtagcagcg cgccgcctga gtcgcccgaa caaaactcgt tgtccttcag cgcgacgatg
     6361 ccgccgtcat cttcgtaatg atcgacgtac gcgtcatccc acatcagaat ctcgatcgga
     6421 cccgtacagc cggatccgaa gtggccatag cccgagatca tcagcttcgt acccggcggc
     6481 gaatacgatg atgcgacctg gaacggcctc acccagctcg gtcgcgatcc aggcgggatg
     6541 cgaatgatcg cgatgtcgcc ctcgatctgc gcacccgacc agctcgggtt gtagtcggga
     6601 ttcgcgatca cgttgccggt gactcgcgga acgcttttgc ccgtcaccgg gtcattgcga
     6661 aacgtcacca tcgcggtcgt caagcagctg ttgctgaaga ccgaattgtt cgtgcagacg
     6721 caatgcgcgg ccgtcaacac cgcggtgtcg gagatcacca ttcccgagca gaacgcatcg
     6781 ctactgtcga tcatgaccat tccggtcaag ttcgtccgga gcaccatgtg cgcgttcttg
     6841 atctcggact cgaaatcgct tgattcgtca cctgcgacag cgcagccaga gagtgcgagg
     6901 agtgcgagaa caaatgccgc cttcatgggg gcgcactgat aacacaagcg tcgaccctta
     6961 attcggttca gcgctgctgc gacgtgccca tcttcacgac aagcttgcgc cgcgctttgc
     7021 ggcccgaagc gtccgtcacg acgatctggt gcgtgccgat cgtcggttcc cagaaatagc
     7081 gctcgttgcc tagggccgtg ccgacgagag cgccgtcgac gaaccacgtg acatctgccg
     7141 agctcgtcga gacttgaaac ggcacctgct ggttcgacgc cggcacaccc gggatgagca
     7201 gcacgatctg gccttccgtc ggcagcgtga tcgtcggcgc ggaggtttgt gcgacgcagc
     7261 cttccgcgaa gtcgggcgcg ttcgggattg cgcgattgcg ttcgcgcagc cacgacgtca
     7321 cctcgctcgg cagcaccgtg aacgactgct tgtcgtagtc gtgatcgtcg aggcggcagg
     7381 cgggcagtac cgcttgcttc gttacgcgat cgacgtcgag catctggtga taggggcacg
     7441 gcgtcgtcgg cacggagtgc atcggcgctt tgacctttac cttgtgcggg caggcgtcgg
     7501 tcgcgatgtg accggagtac gagcagactt cgatctcgat gaggtcggcc ggcggtgcgg
     7561 cggacatcgt cgcgtgacgg cgatcggcga cgccttcgag cacgtcgaac agcagcgggc
     7621 ccgcggcctc ggagccgacg agctcgtggc tcggcgtgtt gtcgacgttg cccgtccaga
     7681 cgacggccgt gtagcgaggc ccggagccga cggaccacgc gtcgcggtag ccaaagctcg
     7741 tgccggtctt ccagtggatc tccgtcggaa tgcccatgtc gcggcgcttc gggaagtcgg
     7801 gacgatcgcg cgtcgagaga tccgtgcgtg tgaggtatgc ggcggcgggc ccgtagatcg
     7861 tcgacgattt gccgcgaggg gcgccggcgc gtagcgcgag cggacggtag acgccgtctt
     7921 cggcgagcgc cgcgtacatg ccggccagct ccatcggcgt gacctccatg ccgcctgcga
     7981 tcagcgagag gccgagctcg cccggctttg cgcgcgcact cgcgacgccc atgcgtgaga
     8041 gctccgcgac gaacgtctcg gtgccgacct cggcgagcag cttgacgaat ggcacgttga
     8101 gcgacttggc gagcgattcg cgcagcgtga cgaggccgag gtagtcgccg tcgaagttgt
     8161 gggggcggta ggcgccgaag tccatgggca cgtcggcgac gaggtagtcg ggcagggcga
     8221 cgccacgatc gatcgcgatc gcgtagagca tcggcttcag cgtcgagccc ggcgagcgcg
     8281 gccgcgcgaa catgccgatc tggccgccgt gcttgaagtc ggttgcgtcg atgttgccga
     8341 cgacggcgac gatttcctgc gtcgtgtgat cggcgacgac gatcgccccg ttgtagatgt
     8401 tcttgcgacg cagctcttcg gcgtgcagcg cgacgacttt ctcgacgagc gcttgcgtgc
     8461 ctttgtcgag cgttgttgcg atgcgcagct cgcccggaga gcgggccttg agccagttcg
     8521 cgacgtgcga cgcctgacgc ggcgttgggc gcagcttcgt cggcggcgca gcgtctttcg
     8581 cttcggcgag cgctgccgtg gcatccgcct gcgagaacac gccggctgca atgagcttgc
     8641 ggaggatcgt gtcgcggcgt tcgcgcagcc gctccgcatt tgtcttcgac
//
LOCUS       DATLBZ010000663         9211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_11102300, whole genome shotgun sequence.
ACCESSION   DATLBZ010000663 DATLBZ010000000
VERSION     DATLBZ010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11102300"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VMZ53_32610"
     CDS             <1..313
                     /locus_tag="VMZ53_32610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33306.1"
                     /translation="SNPATGEETLEVTPRLDLQMTTDHAVLADEAPRYDVTRLLLDGS
                     LRGMADSSQLEVVSGSFSMTTNPSQYGFSATAGQCVTSTSEYDPVTYQDYELYSVGAC
                     L"
     gene            357..1004
                     /locus_tag="VMZ53_32615"
     CDS             357..1004
                     /locus_tag="VMZ53_32615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33307.1"
                     /translation="MRSAIVAVMLAACGRIGFDAERTPDTSLTSACLEESFDQTLTAW
                     NVLDGDGQLDPTGGPDGSGALRANLAGARTVFVAPGLDAVTSATFAADYYLMEQNGDF
                     NVLLVKPGFTGVFPSATGYVTGVHPMGGDNADDVISRYVSGAQTFLVMRPTTLPVRTW
                     RHFDFTRALDGSMSVLIDGAPFMMATDSMVAPPYALYVRVFGVGFVDNVRVDCTR"
     gene            complement(1393..2154)
                     /gene="pgeF"
                     /locus_tag="VMZ53_32620"
     CDS             complement(1393..2154)
                     /gene="pgeF"
                     /locus_tag="VMZ53_32620"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HUS33308.1"
                     /translation="MSEAIKLHTSHVLPREGFVHGFPERFGGVSTGARSSLNLGKRWG
                     DDEANVEENRRLLARHAGYDPAQLQIMRHVHGTAVWKVGEPLPDPAEFDGLVTDKPGP
                     VLGAFAADCIPLLFADPQARVIGSAHAGWRGTVNGIAANVVARMTELGASPATIRVAL
                     GPSIGPCCFEVGPEVVEQFRAAFGDLPNLVVPGPHKPHIDLRVASRAVLERAGVRAEH
                     IDDNPPCTRCDDRFFSYRRDGKDGGVHMAFIAMLR"
     gene            2189..2677
                     /locus_tag="VMZ53_32625"
     CDS             2189..2677
                     /locus_tag="VMZ53_32625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33309.1"
                     /translation="MIPYDDLVAALSAWRARQGLPVSALGGSSSGATPQAGSGPNAVM
                     SQPGSGPYAQGGSGPYAQGGSGPNRGGPPAAPGSGRAKAPSMPPPMGNMSSPTHDDAL
                     DVEDAAMLEEQYETEGNDFAMAFNPNAPVAGDGDDATSIGAAPERPTEADMNRRNDRD
                     DW"
     gene            2674..3525
                     /locus_tag="VMZ53_32630"
     CDS             2674..3525
                     /locus_tag="VMZ53_32630"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HUS33310.1"
                     /translation="MSRTDAANVELVGIAVEASQPSGSAVGIVDDASEGAAVMLGKRV
                     LVGPTDPCGQCEVCRRGGAAVCPHASPRGAVGKRLRVPSRWVVALQDGLELPLPAAAA
                     VAGDVTTAYTLYARTGLGPREPVVVLGENAVARFLVEILRGKGITPTVIVGATSDDAW
                     RSWLLAKGAAVVSGTGDALVASVTGAVSAQGMGNRPWRVIATEASVVPHAAQLAGPRA
                     TLTVLADHETPALPGDLIAREVTVIGVAGPHPDLIVEAAALCVKGEIDLVGGVGDGPT
                     LARVVAS"
     gene            complement(3518..5353)
                     /locus_tag="VMZ53_32635"
     CDS             complement(3518..5353)
                     /locus_tag="VMZ53_32635"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS33311.1"
                     /translation="MKLCPKCQKHFSDDANFCPVDAARLVPLEAQAAGSDSLTSRFEL
                     GQRLGGSRTGTVHKATDKTTGKACVVKLVAPAVVALPGVAQRLDRELKQLERVKSVGV
                     AHVLASGKRSVNGQDETWVATEWLEGAHTLAEAISARGPIPADQAADLIEVVGEALIE
                     AAQVGVVHRDLAPKNVLFASTDSGSVKLINFSLPVPTTDKVPGVPEFVAPEQVEGRPV
                     DQRSNLYSLGALYYYVLTGQMLYAGSAEEVHKQHQTSAIKPPSQLVSVPQPVEAVIMR
                     ALDRSPTKRFLTVRQFVDEVTRVGHGEVIELKATMAMGRANARPKGDLVQTLLGVRGG
                     LPGASSAQTAPLGSSPVQHLANAAMPAPQPTVQAGPATVLSGPPQMPAAQPVPGSGMA
                     AAIASPPTAMAPLTPPSGVDIRSPWAPPGGAPAPAPAPVVVAAPAPAPAPAPAPAPAP
                     APAAFAPPPAAPAAPVIAPQPATTGKKQNARADDGKGKFRETMWFKKGDLDAQAAVAA
                     AEERARTGKDVAADKADSKAIDERYNDDGSITRGDKEKYSLRTGATQMMSAIQDPKNQ
                     KASSSLSKVSEDELMGEMKTLPTWIKIAIPVGILVLLALILWLFS"
     gene            5427..5585
                     /locus_tag="VMZ53_32640"
     CDS             5427..5585
                     /locus_tag="VMZ53_32640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33312.1"
                     /translation="MASNTKATTVKRKNKHEKAGRRRKNKQAKKSTKSATELFANLGE
                     PGKPAPRR"
     gene            5595..6062
                     /locus_tag="VMZ53_32645"
     CDS             5595..6062
                     /locus_tag="VMZ53_32645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33313.1"
                     /translation="MKRIARVASIALLVVGAIAAITLVAWSIYVRQSFFGPGPGRPGL
                     RTGLELAARLGPEYLIALGAMAAGALALAYMQWRERKLAFLIGAISAALAIAGAWWGR
                     HFIVGLEDPHFVKKSPWVQAATFVATIATVYLGALAASSLIAWLTARKSRPSE"
     gene            6076..7509
                     /locus_tag="VMZ53_32650"
     CDS             6076..7509
                     /locus_tag="VMZ53_32650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33314.1"
                     /translation="MRRCLVLLGLCGCDALFGIDTLDYKPDAPVDTAPRITVTGHAVK
                     RWLHNNAAHEPTLEPAIHPAMPIAVHLADGSVPPTTWDPATSEFSFEAGVGERYAVTY
                     ATNLGSYEYQMTTPTLTLQDVELGHPDQVAPGAQAVVQWLPTSGTTLAAADVRFASTG
                     VWTNTVPTSPATNNFIVNWPSAVVVGGQRPALLSAAAHDSLYFLHYTTVATTPPHRVV
                     DQYAEVTDAAQDANTTTTYINALQPLPRTDCVDLHVRRAAEFARLSQYPKFATPGGTT
                     QILADAMPELGFRAPHELALITSTTDTNVAVSYTNPFPGFGTMIAENNYLSRQITGGG
                     TISVNTQYIDLITPTSPCMRYDDPPPPPFVMTPSLAGVPLADDIVIGIDASKLVELTW
                     SWDSEPAAVTQVNLMKGASELVRVFFSTDGKILIDPALLDANTAYRLVLAPYHGYFPN
                     VAEGDFATRTYPARLSILMTGYFKVAK"
     gene            complement(7510..>9211)
                     /locus_tag="VMZ53_32655"
     CDS             complement(7510..>9211)
                     /locus_tag="VMZ53_32655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HUS33315.1"
                     /translation="ERIAAETMQIYAPLANRLGIQWVKVELEDLAFKYIYPGEYEQLA
                     GEVAKTRAERLEYIHHVEKLILKEMQDNGVPCEVYGRAKHLWSIYTKMKRTQRPFEEI
                     HDSIGFRVITDTQMHCYQALGVAHQTWTPIPGRFKDYIALPKPNLYQSLHTAVIGPRG
                     ERIEVQIRTKEMNLVAEHGIAAHWKYKEGKPLALEDDKKFAWLRQLMESQKELRDPTE
                     FLESVKIDLFGDEVYVFTPGGDVKALPKGSCPIDFAYAVHSSIGDHCSGARVNGMIVP
                     LRYQLRNGDTIEIITSPNQKPNKDWLKLVKTARARTKIRWLLRQEQRDKAVALGTDLI
                     DKALRKHDTSIAKADKQLKHAAQELKCQTVDELIVQVGYGKITVQQVLEKALPALAKR
                     ADGEAAPKAESMLKTLLRKVTRRTTSSGIKVAGEEDILVRFAKCCSPLPGDPIVGFIT
                     RGRGVTVHRRDCDKGLDLDPERRIDVEWDGLSKAQHEVAIQVLCTNKPGLLAHISQSF
                     TDQGVNISQAHCRATEDGRAVNTFHASVRDLDQLKSVIRSLSRIKGVFSVDRVSADPS
                     "
BASE COUNT         1464 a         3066 c         3108 g         1573 t
ORIGIN      
        1 gtcgaacccc gccacgggcg aagaaacgct cgaagtcacc ccgcgcctcg acctccagat
       61 gacgaccgac cacgcggtgc tcgccgacga agcgccgcgc tacgacgtga cgcgcctcct
      121 cctcgacggc tcgctgcgcg gcatggccga ctcgagccag ctcgaggtcg tgtcgggctc
      181 gttctcgatg acgaccaacc cgtcgcagta cggcttctcg gcgacggccg gccagtgcgt
      241 cacgtcgacg tcggagtacg acccggtgac ctaccaggac tacgagctct actccgtggg
      301 cgcctgcctc tagctcgctg acgcctcggc gtcgcgctag gttatgctcg tcgacggtgc
      361 gctccgcgat cgtcgccgtg atgctcgcgg catgtggccg catcggcttc gatgccgagc
      421 gaacgccaga cacctccctg acgagcgcgt gcctcgagga gtcgttcgat cagacgctga
      481 ccgcgtggaa cgtgctcgat ggagacggcc agctcgaccc caccggcggg ccggacggtt
      541 ctggtgcgct ccgcgcgaac ctggcgggcg cgcgcaccgt attcgtcgcg ccgggcctcg
      601 atgcagtgac aagcgcgacg ttcgccgccg actactacct catggagcag aacggcgact
      661 tcaacgtgct cctcgtgaag cccggcttta ccggcgtctt cccgtccgcc accggctacg
      721 tgaccggcgt gcacccgatg ggcggcgaca acgccgacga cgtcatctcg cgatacgtca
      781 gcggtgcgca gacgttcctg gttatgcgcc cgaccacgtt gccggtgcga acatggcgcc
      841 acttcgactt cacgcgcgcg ctcgacggat cgatgagcgt cttgatcgat ggcgcgccat
      901 tcatgatggc caccgactcg atggttgcgc cgccctacgc cctctacgtt cgagtctttg
      961 gagtcggctt cgtcgacaac gtccgcgtcg actgcactcg ctagaccatc acgcgcgcga
     1021 tcagcaggcg ccgcggcgcg acacgattgg cgggtgcggg cgaatcggcg cgggcatggc
     1081 gcggagccga gcgcggcgtc gtctgagggc cagccgcgga gagcgagcgt aggcgcgcga
     1141 cgagacgctc ggccccgccg tcagctctcc acagggcctg cgcagcaggc caacgatcgt
     1201 gcaacgacga gaaccggaga acgggagcgc cgtcacgcga gcgggtccgg ggcggccctc
     1261 aggcggcgcc gccctcggcg ttcgcgttct tcgccggggc gtttcgatgc gcgcgagcgc
     1321 tagcgaaagc gcgatcagcg gccgcccgag cgggcagcac gacggcgcgg cgcaacgcga
     1381 cgacggaagc gatcagcgaa gcatcgcgat gaacgccatg tgcacgccac cgtctttgcc
     1441 gtcgcggcgg tagctgaaga agcggtcgtc gcagcgcgtg cacggcgggt tgtcatcgat
     1501 gtgctcggcg cggacgcccg cacgctcgag caccgcgcgc gaagcgacgc gtagatcgat
     1561 gtgaggcttg tgagggcctg gcacgacgag gttcggcaga tcgccgaacg cggcgcggaa
     1621 ctgctcgacg acttcggggc cgacctcgaa gcagcacggg ccgatcgatg ggccgagcgc
     1681 gacgcggatg gtggcgggcg atgcgccgag ctcggtcatg cgcgcgacga cgttggccgc
     1741 gatgccgttg acggtgccgc gccagccggc gtgagccgag ccgatgacgc gggcttgtgg
     1801 gtcggcgaag aggagtggga tgcagtcggc cgcgaacgcg ccgagcacgg ggcctggctt
     1861 gtcggtgacg aggccgtcga attcggctgg atcgggcagc ggctcgccga ctttccagac
     1921 ggctgtgccg tggacgtgcc gcatgatctg gagctgggct gggtcgtagc ccgcgtggcg
     1981 cgcgaggagg cggcgatttt cttcgacgtt ggcttcgtcg tcgccccagc gcttgccgag
     2041 gttcagcgac gagcgcgcgc cggtcgagac cccgccgaag cgttcgggga agccgtgtac
     2101 gaatccttcg cgtgggagga cgtgcgacgt atggagcttg atcgcctcgc tcaccgatcg
     2161 gcttctagca cgtgatatcg ttggttccat gatcccgtac gacgatctcg ttgcggcttt
     2221 gtctgcgtgg cgtgcgaggc aagggcttcc cgtcagtgcg ctcggcggaa gtagctcggg
     2281 tgcaacgccg caggctggtt ccggtcccaa cgcggtgatg tcgcaacctg gctcgggacc
     2341 ttacgcgcag ggtggctcgg gaccgtacgc gcagggtggc tcgggaccga atcgcggtgg
     2401 accgcccgct gcgcccggct cgggtcgcgc gaaagcgccg agcatgccgc ctccaatggg
     2461 caacatgagc tccccgacgc acgacgacgc actcgatgtc gaagatgcgg cgatgctcga
     2521 agagcagtac gagaccgagg gcaacgattt tgcgatggcg ttcaatccga acgcgcccgt
     2581 tgccggcgat ggcgacgacg cgacatcgat tggcgctgcc cccgagcgcc cgaccgaggc
     2641 ggacatgaac cgccgtaacg accgcgacga ctggtgagcc gtaccgacgc tgcgaacgta
     2701 gagctcgtcg ggatcgcggt cgaggcttct cagcccagcg gttctgccgt cggcatcgtc
     2761 gacgacgcga gcgaaggcgc agccgtgatg ctgggcaagc gcgtgctcgt cgggccgacg
     2821 gatccgtgcg ggcaatgtga agtgtgccgg cgcggcggtg cagcggtatg cccgcacgca
     2881 tcgcccagag gtgcagtcgg caagcgactt cgcgtgccga gccgatgggt cgtcgcactg
     2941 caagatggcc tcgagcttcc gctgcctgca gcggccgccg tcgcgggtga cgtgacgacg
     3001 gcgtacacgc tgtacgcgcg caccgggctt ggccctcgcg agccggtcgt cgtgctcggc
     3061 gagaatgcgg tcgcgcgatt tctcgtcgag attttgcgcg gcaagggcat cacgccgacc
     3121 gtgatcgtcg gcgcgacctc cgacgacgcc tggcgatcgt ggctgctcgc aaagggcgcg
     3181 gcggtcgtca gcggcacggg cgacgcgctc gtcgcatcgg tgacgggtgc ggtctccgcg
     3241 caaggaatgg gcaatcggcc ttggcgcgtg atcgccacgg aggcgtccgt cgttccgcac
     3301 gccgcccagc tcgccggtcc acgcgcgacg ctcaccgtgc tcgccgatca cgaaactcca
     3361 gctctaccgg gcgacctgat cgcgcgcgaa gtgaccgtca tcggtgtcgc cgggccgcat
     3421 cccgatttga tcgtcgaggc ggcggcgctc tgcgtgaagg gcgagatcga tctcgtcgga
     3481 ggcgtcggcg acggccccac gctcgcgcgc gtcgtcgcta gctaaagagc cagaggatca
     3541 gcgcgagcag cacgaggatg ccgaccggga tcgcgatctt gatccacgtc ggcagcgtct
     3601 tcatctcgcc catcagctcg tcctcggaga ctttgctgag gctcgagctc gccttctgat
     3661 tcttcggatc ctggatcgca gacatcatct gcgtcgcgcc cgtgcgcagc gagtacttct
     3721 ctttgtcgcc gcgcgtgatc gagccgtcgt cgttgtagcg ctcgtcgatc gctttcgagt
     3781 cggccttgtc ggccgcgacg tctttgcccg tgcgcgcgcg ctcctcggcc gcagcgaccg
     3841 cggcttgcgc gtcgaggtcg cccttcttga accacatcgt ctcgcggaac ttgcccttgc
     3901 cgtcgtcggc gcgagcgttc tgcttcttgc ccgtcgtggc cggctgcggc gcgatcaccg
     3961 gtgcagcagg cgcagcgggc ggcggcgcga acgcggccgg tgcaggcgct ggcgctggag
     4021 ctggagctgg agctggagct ggagcgggcg cggcgacaac aaccggcgcg ggggccggcg
     4081 caggcgcacc accgggcgga gcccacggcg agcgaatgtc gacacccgac ggcggcgtca
     4141 gcggcgccat cgcggtcggc ggcgatgcga tcgcagcggc catgccggag ccgggaaccg
     4201 gctgcgcggc cggcatctgc ggcgggcccg aaagcaccgt cgcgggacct gcttgcacgg
     4261 ttggctgcgg cgcgggcatc gcggcgttcg cgagatgctg cacgggcgac gaaccgagcg
     4321 gcgccgtctg tgcggagctc gcacccggca gaccaccgcg cacgccgaga agcgtctgca
     4381 cgagatcgcc cttcgggcga gcattcgcgc gtcccatcgc catcgtcgcc ttcagctcga
     4441 tcacttcgcc gtggccaacg cgcgtgacct cgtcgacgaa ttggcggacc gtcaagaagc
     4501 gcttcgtcgg gctgcgatcg agcgcgcgca tgatgacggc ctcgaccggc tgcggcacgg
     4561 acacgagctg cgacggcggc ttgatcgcgc tggtctgatg ctgcttgtgg acttcttcgg
     4621 ccgagcccgc gtagagcatc tggccggtga gcacgtagta gtagagcgcg ccgaggctgt
     4681 agaggttcga gcgctgatcg acggggcgac cttcgacctg ctccggcgcg acgaactcgg
     4741 gaacgcccgg cactttgtcc gtcgtcggca ccggcagcga gaagttgatc agcttgacgg
     4801 aacctgagtc cgtcgatgcg aacagcacgt tcttcggcgc gaggtcgcga tgcacgacgc
     4861 cgacctgcgc ggcttcgatc agcgcttcgc cgacgacctc gatcagatcc gcggcttgat
     4921 ccgccgggat gggtccacgc gccgagatcg cttcggcgag cgtatgcgcg ccttcgagcc
     4981 actcggtcgc aacccacgtt tcgtcttggc cgttgaccga gcgcttgccc gacgcgagga
     5041 cgtgcgcgac gccgacgctc ttcacgcgct cgagctgctt gagctcgcgg tcgagccgct
     5101 gcgcgacacc cggcagcgcg accacggccg gggccacgag cttgaccacg cacgccttgc
     5161 cggtagtctt gtccgtcgct ttgtggaccg ttccggtgcg agatccgccg agccgttggc
     5221 cgagctcgaa cctcgacgtc agcgagtcag acccggcggc ctgggcctcg agtggcacga
     5281 ggcgggcggc gtcgacgggg cagaagttcg cgtcgtcgct gaaatgtttc tggcacttgg
     5341 ggcagagctt catcgcgggg gtccccaggg attgcaacgt aacacacgat tgcgctagaa
     5401 accccggtct ccacaaagga tctgtcatgg catcgaacac caaggcgacc acggtcaagc
     5461 gcaagaacaa gcacgaaaaa gccggccgcc gccgcaagaa caagcaggcg aagaagtcga
     5521 cgaagtcggc gaccgagctg tttgcgaatc tcggcgagcc cggcaagccg gctccgcgtc
     5581 gttaatctga tcgaatgaag cgcatcgccc gagtagcgtc gatcgcgctg ctcgtggtcg
     5641 gcgcgattgc ggcgatcaca ctcgtcgcgt ggtcgatcta cgtccgccag agcttcttcg
     5701 ggccgggccc agggcgacct ggcctgcgca ccggtctcga gctcgccgca cggcttgggc
     5761 ccgagtacct gatcgcgctc ggcgcgatgg ccgcgggtgc actcgcgctc gcgtacatgc
     5821 agtggcgtga gcggaagctc gcgttcttga tcggcgcgat cagcgctgcg ctcgcgatcg
     5881 ccggcgcctg gtggggacgc cacttcatcg tcgggctcga ggatccccac ttcgtaaaga
     5941 agagcccgtg ggtccaagcg gcaacgttcg tcgcgacgat cgcgacggtg tatctcggag
     6001 cccttgcagc gtcctcactg atcgcgtggc ttaccgcacg taagtcacgg ccctcggaat
     6061 gacatactga cgacgatgcg tcgctgcctc gtgctgctcg gcctctgcgg ctgcgacgcg
     6121 ctgttcggca tcgacacgct cgactacaag cccgacgcgc cggtcgacac tgcgccgcgc
     6181 atcaccgtca cgggccacgc cgtgaagcgc tggctgcaca acaatgcggc gcacgagccg
     6241 acgctcgagc ctgccattca tccggcgatg ccgatcgccg tgcatctagc ggacggcagt
     6301 gtcccgccga cgacgtggga tccggcgacg agtgaattct cgttcgaagc cggcgttggg
     6361 gagcgctacg cggtgacgta cgcgacgaac ctcggctcgt acgaatatca gatgacgacg
     6421 ccgacactca cgctgcagga cgtggagctc ggtcatcccg atcaggtcgc gccgggcgcg
     6481 caagccgtgg tgcagtggct gccgacgtcg ggcacaacgc tcgcggccgc ggacgtgcga
     6541 ttcgcatcga cgggcgtgtg gacgaacacc gtgcccacgt cgccagcgac gaacaacttc
     6601 atcgtgaatt ggccatcggc cgtcgtcgtc ggaggccagc gccctgcgct gctctcggcc
     6661 gcagcccacg actcgctgta cttcctccac tacacgaccg tcgctacgac accgccgcat
     6721 cgggttgtcg atcagtacgc ggaggtcacc gacgccgcgc aagatgccaa cacgacgacg
     6781 acctacatca acgcgctgca gccgctgccg cgtacggatt gcgtcgactt gcacgtgcgt
     6841 cgcgctgccg agtttgcgcg gctctcgcag tatccgaagt tcgcaacgcc cggaggcacg
     6901 acgcaaatac tcgcggacgc gatgcccgag ctcggctttc gcgcgccgca cgagcttgcg
     6961 ctgatcacga gcacgacgga tacgaacgtc gcagtgagct acacgaaccc gtttccgggc
     7021 ttcggcacga tgatcgccga gaacaactac ctctcgcgtc agatcaccgg cggcgggacg
     7081 atcagtgtca acacgcagta catcgacctc atcacgccga cgtcgccgtg catgcgttac
     7141 gacgatccgc cgccgccgcc cttcgtgatg acgccatcgc tcgccggtgt gcctctcgcc
     7201 gacgacatcg tgatcggaat cgatgccagc aagctcgtcg agctgacgtg gagctgggat
     7261 agcgagcccg cagcggtcac ccaggtgaat ctgatgaaag gcgcgtccga gctcgttcgc
     7321 gtcttcttct ccaccgacgg taagatcttg atcgacccgg cgctgctcga cgcgaacacg
     7381 gcctatcggc tcgtcctcgc gccctatcac ggctacttcc cgaacgtggc cgaaggcgac
     7441 ttcgcaacgc gcacctatcc agcacgcctc agcatcctga tgaccggcta cttcaaagtc
     7501 gcaaagtagc tagctcggat ccgcggacac gcgatcgacg ctgaacacgc ccttgatgcg
     7561 agacagcgag cgaatcacgc tcttcagctg atcgagatca cgcaccgacg cgtggaacgt
     7621 gttcacagcg cggccatctt ccgtcgcgcg gcagtgcgct tggctgatgt tgacgccctg
     7681 atccgtgaac gactgcgaga tgtgagcgag caggccgggc ttgttcgtgc agaggacttg
     7741 gatcgcgacc tcgtgctgcg ccttgctgag gccgtcccac tcgacgtcga tacgccgctc
     7801 gggatcgaga tcgagaccct tgtcgcaatc gcgacgatgc accgtgacgc cgcggccgcg
     7861 cgtgatgaag ccgacgatgg gatcaccggg cagtggcgag caacacttcg cgaaccgcac
     7921 gaggatgtct tcctcgcccg cgaccttgat gccactcgac gtcgtgcggc gcgtgacctt
     7981 gcgcagcagc gtcttcagca tgctctcggc cttcggtgcg gcctcgccat cggcgcgctt
     8041 cgcgagcgca ggcagcgcct tctcgagaac ctgctgcacc gtgatcttgc cgtagccgac
     8101 ttgcacgatg agctcgtcga cggtctggca cttgagctct tgcgccgcgt gcttgagctg
     8161 cttgtccgct ttcgcgatcg acgtgtcgtg cttgcgcagc gccttgtcga tgagatcggt
     8221 gccgagcgcg actgccttgt cacgctgttc ctgacgcaac agccaccgga tcttcgtgcg
     8281 tgcgcgcgcc gttttgacga gcttgagcca gtccttgttc ggcttctggt tcgggctcgt
     8341 gatgatctcg atcgtgtcgc cgttgcgcag ctggtagcgc agcggaacga tcatgccgtt
     8401 cacgcgcgcg cccgagcagt gatcgccgat cgagctgtgc accgcgtacg cgaagtcgat
     8461 cgggcagctg cccttcggca acgccttcac atcgccgccg ggtgtgaaca cgtagacctc
     8521 gtcgccgaag aggtcgatct tgacggactc cagaaactcc gtcggatcgc gcagctcttt
     8581 ctgcgattcc atgagctggc ggagccacgc gaacttcttg tcgtcctcga gcgcgagcgg
     8641 cttgccctct ttgtacttcc agtgcgcagc gatgccgtgc tctgcgacga ggttcatctc
     8701 cttcgtgcgg atctggacct cgatgcgctc gccgcgcgga ccgatcacgg ccgtgtggag
     8761 cgactgatac aagttgggct ttggcagcgc gatgtagtct ttgaagcggc cgggaatcgg
     8821 cgtccacgtt tgatgcgcga cgccaagcgc ttgatagcag tgcatctgcg tatcggtgat
     8881 gacgcgaaag ccgatcgagt cgtggatctc ttcgaagggg cgctgcgtgc gcttcatctt
     8941 cgtgtagatc gaccagaggt gctttgcgcg gccgtacacc tcgcacggca cgccgttgtc
     9001 ctgcatctct ttgaggatca gcttctcgac gtggtggatg tactcgagac gctcggcgcg
     9061 cgtcttcgcg acttcacccg cgagctgctc gtactcgccc ggatagatgt acttgaacgc
     9121 gagatcttcg agctcgacct tgacccactg gataccgagg cggttcgcga gtggcgcgta
     9181 gatctgcatc gtctcggcgg cgatgcgctc c
//
LOCUS       DATLBZ010000664        18839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7382410, whole genome shotgun sequence.
ACCESSION   DATLBZ010000664 DATLBZ010000000
VERSION     DATLBZ010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18839
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7382410"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1292)
                     /locus_tag="VMZ53_32660"
     CDS             complement(99..1292)
                     /locus_tag="VMZ53_32660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33316.1"
                     /translation="MALAALVPLVPSIARADDAADFRDASTRRDIDALERIGATSSMW
                     ADDAWVEAARIAEANADYARARRDLEKAIETSRALARARGNASIGSGATSTTGASGDS
                     ILVQRAQADLARLSRVAGQSGEWGAVIAEEERLLPALQSPGDPRAALDALGKLAREHP
                     GYPRNAMLMVQIAAGWEREGDADRALAWLADADRAAIDPLDRLRVRADRVRVLIRAGR
                     ISQAEAELAALAANSAGANGAGANVAASPGLIANLRAALERAKLRRDIRWAMWALLAL
                     LAIAATVLLRRGAGSWRAAGRRLVRPPVEAIFFVPIAGVLIAVASTGNPLVGRAVWGI
                     AIAGVVTSWVTGALLAATRVTLKRAVAHALCAMLAVGAATYVAVDRGHLVDFLVETWR
                     EGPDR"
     gene            complement(1324..2619)
                     /locus_tag="VMZ53_32665"
     CDS             complement(1324..2619)
                     /locus_tag="VMZ53_32665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUS33317.1"
                     /translation="MRALPLLSLLLLVSACDDDEEDTTFTDTGGTLEIDGCGYSITTR
                     IGAEPPKVSGKIIGMDPTPRLVHLGIVGDPKTSIVAQWRTMDDTTTAGSIRWAQGANL
                     PAAQLTNETKGVRFRYEGTGSAMYMVHQAHLCSLEAGTTYSYQVGTGDSWSPVYTFHT
                     APDVTAHPDAETVFGYVGDSRGGYDVWAQLATQIQQRMPDLVLFSGDATTIGLAQPEW
                     EEFQAAAEPLFASTPVVLTNGNHEANAINFYSQFAMPGDQENFGFTYGFSRIFVGNDS
                     PDNPSDITGVIPQAMDADFAAHTDARWRMMMHHRCQWSSGTRHGSDPALQAAFGPIVD
                     KHKLDLVLNGHEHMFEMTKPLFGGQVVTSNQQGTVYVVAGGAGAELYGFGTPAVWSQY
                     VESVHTAAVIRARKDTLTMETFRADGTAVPSGAYSKTHL"
     gene            2661..3566
                     /locus_tag="VMZ53_32670"
     CDS             2661..3566
                     /locus_tag="VMZ53_32670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS33318.1"
                     /translation="MVQSCLLLALAITVAACGGKANPAPGGDDDGSADDGVFVNQAGF
                     EPTAFTVEVSGEGRPIIFIPGLGCPGEMWNDVVEKLSDRYESHVLTLAGFAGNDPIKP
                     PLGAKVRKELIRYIRSHKLESAIVVGHSYGGFIAYWLAETAPRDVLGGVVIVDAGPGG
                     EANDEEARLLRNAWAQAGDDELPRQIRAKFSMMTASPQKMEPFIAEIAKSDRQTIGDA
                     IFEYVKTDIKDDVERIKAPALIVLADGGLQGRYRAEASDIPDHEVVVLKGAKHFVWFD
                     DPDGFINALDTFIRRTDEHDSSASR"
     gene            3538..4218
                     /locus_tag="VMZ53_32675"
     CDS             3538..4218
                     /locus_tag="VMZ53_32675"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HUS33319.1"
                     /translation="MSTTPPPAAKANLERKRLPWRSLSLFAIAVLATVLFVWIASELR
                     EGELDKIDTRIAIAVHHDQNVVLDVLAIAFTLLGSGPILLSAVAAVCFWAYRRGRRSY
                     IVIVIANCFIAMVLNPLLKLVFERARPTLFEVISRPDTYSFPSGHSMSAVTVYGALAA
                     VIIALRPTVKPIAVIVAMIFIVGIGLSRVYLGAHWPMDVIAGWASGVPFLVTTVYLLH
                     RAKRGEAK"
     gene            complement(4219..5373)
                     /locus_tag="VMZ53_32680"
     CDS             complement(4219..5373)
                     /locus_tag="VMZ53_32680"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUS33320.1"
                     /translation="MPLAGAPYWRTVLYFEPGGKLSAGAPAIARYLETDGEGRLVQSI
                     KSHLASESFSKTTIFGRRWALDDMIAAYLRQIRAASSVDLGSRCVVGRPVRYWGADDE
                     EDDARAVGRMKDALAKAGFTDVVFEYEPVGAAARYAAKLDHEELIVVADYGGGTTDFS
                     AIRVGAGKARVLATGGVGISGDAFDARVIDAVVAPALGRGTLYTVDGFGGEAQVPAWI
                     YSSLRRWHLLSFLKEDSTQRLLDRVASGTRDPVKIGRLVSVVEEDLGLVLHRSVEGAK
                     VELSKQDAAKVALAQITLDVPVTRADFDSWIDEDLDAIDRVLDDVLAKAGVAASAVDR
                     VFATGGSSLVPAVRGRLTSRFGEDKVVGGEELTSVAWGLAARAREVFGGG"
     gene            complement(5453..6364)
                     /locus_tag="VMZ53_32685"
     CDS             complement(5453..6364)
                     /locus_tag="VMZ53_32685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33321.1"
                     /translation="MIRASIVLLALLAGTAYAQPGATPSGDMPPPPPEQQQPPPYGPQ
                     GMPAPMPPDQPPPPQNDDALIDQAIGKLQAEMPTIETVARGTFNRARRAVAIGPTVGV
                     WSAAYLTPGEFDAAFSVGLQLQTFKVPIVPDTETIKALIIERLKAVAKDRIKAAFQGR
                     PIDPVSAEQIARQVYADVRAEVLGLANTRAKTLERPGYTVAFEADRRFGAERWLGRTR
                     VGVGIWKLSFSLSASFGRACRGTDCDDRIRGFIGPEVTLHIIPKKNPRANVVDVFLRS
                     DFQATGRAETTTYDQLVLGLRYQIDVI"
     gene            complement(6394..8037)
                     /locus_tag="VMZ53_32690"
     CDS             complement(6394..8037)
                     /locus_tag="VMZ53_32690"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HUS33322.1"
                     /translation="MPTFAYQELLPVGHHDDTPFKKLTGDFVSTFEAKGKSFLDVQPE
                     ALTLLAQTAMRDIAHLLRPGHLAQLRKILDDPEASPNDKFVALELLKNANIAAGGVLP
                     GCQDTGTAIVMGKRGERVVTGGTDEEAISRGISTTYTETNLRYSQVAPLDMFEEKNTG
                     TNLPAQIEIYGTAGDEYHFLFMAKGGGSANKSILFQETKALLNPKSLAAFLDQKLRSL
                     GTAACPPYHLAVVIGGTSAEYTLKVAKLASARYLDHLPTKGSIAGHAYRDLEWEQKVL
                     EISRQTGIGAQFGGKYFCHDARVIRLPRHGASCPVALAVSCSADRQALGKITRDGVFL
                     EQLETDPAKYLPDITHEELPGEVVKIDLTMPMNEIRATLSRYPIRTRLSLTGPLIVAR
                     DIAHAKLKERIDAGQGLPQYMKDHCVYYAGPAKTPQGYASGSFGPTTAGRMDSYVDLF
                     QANGGSMVMLAKGNRSREVTDACKKHGGFYLGSIGGPAARLAKDCIKSVTVLEYPELG
                     MEAVWKIDVVDFPAFIVVDDKGNDFFADISGGGKKLPVK"
     gene            complement(8095..9453)
                     /locus_tag="VMZ53_32695"
     CDS             complement(8095..9453)
                     /locus_tag="VMZ53_32695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33323.1"
                     /translation="MRAFRAALLVAVAGAHLGGRDARADERLDLQLPDVPLLEPAFHL
                     DRDVPYDERTKSGRTYFIGIAGARADDGGTFDPQTGRTDGDARAAFMGSGALGLGYHS
                     DSLTAAWRSELYGLSGRGVRGRHRGLVQGRILIGGDFGLVETVSANAEHGDARALAPV
                     RTGPSERASGEAAVEVLVPLGKKKSDLQMVIVAGASYGGTAWETHDAPTLDSENHNTT
                     MIGIAAAPADGELPRGRIDIVRLNIEGQRVRFRPGVMPIDMTQPIGPGNGGAETRHID
                     VLVGAKDMTLYIDHEMFAVFDAYLGGSWVEAENAAGTINDSLFNMRFATALKWKPKSD
                     GDTRGLGVNISRNPTTTPDAQRILAEVRFELASSIESDSMLFGARAGISWLKPMEGGP
                     TQVETLVRYGMEVEAFWKLPMGVEAGIYHANSFEPRVTGDPWATPRRWAVESGGIVRL
                     RH"
     gene            complement(9609..11459)
                     /locus_tag="VMZ53_32700"
     CDS             complement(9609..11459)
                     /locus_tag="VMZ53_32700"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS33324.1"
                     /translation="MREQDVVDGRYRIERKLAAGGFGAIYAATDLIMGREVAIKVLHR
                     ELASDALLVARFRREAEALARLRDPHTVTMYDVGADADGTPYIVLELLRGETLYQHFL
                     NTGPLPWRRMAQIAHGVCSSLREAHAVGIIHRDLKPANIHLEVSALQQDQAKVLDFGI
                     AKLVDAETAALPDLTLAGQMIGTFDYMPPEQLIGGACSGKSDVFALAVVMYEMIAGQL
                     PFSPAQGPASRLMAMLTTPPVPLAERANVPPHLDAIIMRALSHEIDERPDMEELDNVL
                     VQLIEGTKPFVAPRASSDATSDASSEINALIDEALRDESDDGAFPDDDGDTWVGAPPQ
                     STTIGLPPTRPAFDAEGTGQVRAPSQRFAVGSRTEIPPASNPYVTPRRVTPAHVPPPP
                     PRAPARNVTPVDPMASTMSPSAGVKAIDPLASTMSPDAPRAPSPRAPSPRAPSPRAPS
                     PHLHIDARSSTNADSQAQTARQPRRVLTPPSGTSTNTASARAEIERLANRSRVPVVRI
                     VTPPQGHARAGSDMGRAGSDMGRASHDAFTPPAHRVWQGRPTPSDMALPPAPSFAPSG
                     ELPTAPSRMATGTTPIVNQPTSISMARVALWALVLFALGFAAAIAVASLA"
     gene            complement(11576..13219)
                     /locus_tag="VMZ53_32705"
     CDS             complement(11576..13219)
                     /locus_tag="VMZ53_32705"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HUS33325.1"
                     /translation="MSFAYTFGWTLVLLFLVELITGAALAAFYSPSSTDAWASVAYVQ
                     DRMTAGWFVRGMHVHAASALVIVSGIHLLQAAIFGAYKRPRELTWWLGVFLMLLVLGF
                     TITGYVLRWDQAGFWANQVEVGIAAGTPVLGGVIRKFLIGGNEYGNLTLTRFYMLHVV
                     LLPAIVTVLTIGHIVVARRHGATAHWKRDPDEAPVRRWPHQSFLNVAAMMLVIAVLVF
                     WTNFTHGADLAAPADPSAAYDARPLWYFRWLYALRSIAGSAEQIVAMIAPAIVVGLLV
                     LLPLLDRSNTRAPGKRLVWVGATVALFAVIGLLTMSSFMHDSSDKALAKRQHEEEVKA
                     GRLRALAAMKGVPVTGPADLRTTIPMYRARTLWAQRCAECHDADSKDRKGPIIAAGHG
                     SRDWVKGFLKDPNGAAYWGKTKLAKTEDAMKPVDLQGAELDDLVEAIYAESGATDVIP
                     ARRERGRGVFEKACTDCHSLDEGVAGGSGPGLGGLGSRDWYTHFIGNPKSPVHMGPEH
                     SEMPRFDKDLSIIDRDALAEYLLWLRTATPGDVAKLEPL"
     gene            13203..14291
                     /locus_tag="VMZ53_32710"
     CDS             13203..14291
                     /locus_tag="VMZ53_32710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33326.1"
                     /translation="MYANDTPGTTDGARSPRPSRSANHAATPRLRGGVLPSRRSLLAA
                     AGLPGTAGRDDATAGLDDAAAGLDAATALADGAALAAIALADGPPLVDDPPLVDDAPL
                     VDDPPLGDDAPLGDAPPLVDDPPLVDDPPLVDDPPLGDDAPLVDNPLDVAAAAASVAL
                     PASPAACVRFSDAPVARPLARDAPVVDVPVVDGPVVDGPLVDGPVVDGPLVDAPLVDG
                     PLVGGGDAVIDGSVVVSLVAGSSAGPLASDGSVVGTPDDGSTASPEVRIPSGGVAVPG
                     GRLIAGLVCDLRFFVPPLAPPPPPPAPPPPPPPPPPAPPPAPPLFGVPPLPPLLPLPL
                     PVLVTPAPAMRCRACSGPTSGAGGRSPP"
     gene            complement(14215..14742)
                     /locus_tag="VMZ53_32715"
     CDS             complement(14215..14742)
                     /locus_tag="VMZ53_32715"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HUS33327.1"
                     /translation="MSEDDPDRRKFLKLATCGLGGIVGIAATAPAVSLVLAPTSRQTV
                     TTPTDPIDLGDATIAKGWSDWRKVDVIAPEVRDGWTTARNIVLGAAYIRATSTPDQFE
                     ALSGVCPHLGCAVGWEGKSFLCPCHDSKFAADGAKQTGPSLRGLDPLPIKVENGRLRL
                     TWVRYKLDTASREPA"
     gene            complement(14815..15213)
                     /locus_tag="VMZ53_32720"
     CDS             complement(14815..15213)
                     /locus_tag="VMZ53_32720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HUS33328.1"
                     /translation="MSTTAATSSKALTKKELKKFEELLEEKRKAVLERAREMLAVENM
                     ALDTNDLPDEMDLASSEYLQSFEFRLRGREKSLLSKLDLALKKIDDGTFGVCEVCEEP
                     IGKKRLEARPETTLCIKCKEDQEREERTMG"
     gene            15344..15544
                     /locus_tag="VMZ53_32725"
     CDS             15344..15544
                     /locus_tag="VMZ53_32725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HUS33329.1"
                     /translation="MAGSDKRKQSLYFPEDMLKEIQDEATRQDRSLSWIVQKAWKIAR
                     KEIMKYPSVNEFPGEEDEKEIA"
     gene            15677..16486
                     /locus_tag="VMZ53_32730"
     CDS             15677..16486
                     /locus_tag="VMZ53_32730"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUS33330.1"
                     /translation="MCDGIGCDGYVWRYLREDLTDRFGLHPHYRGHGRTTAPRDPTRV
                     RIEDLADDIAAVLDDASVEQAVLIGHSMGVQVALETYRRHAARVAGLVLVCGAPSHPL
                     KTFRGSSTLEELLPMFEKWIHRVPGVVNRLTRTLLPTRLAYEVASRLEIRRELVEPSD
                     FMPYLEGMSRIDARLFVAMLSSAGQHSAEDLLPSINVPTLVIAGERDGFTPPDRSRAM
                     AGAIPGAELLEIEGASHTAPIERPSLVDSRIRDFIRGRVDAPTDRGISPQS"
     gene            17047..17397
                     /locus_tag="VMZ53_32735"
     CDS             17047..17397
                     /locus_tag="VMZ53_32735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33331.1"
                     /translation="MVALPPVPLVRQRLRASVKEFATSQPGRRAAALAAVWIAATGCE
                     ADLNHYDPEEALRTYRLIESELRAELRISLGRAITNEPHAATRNTMISMLEHLEELEA
                     AAVAPRPARRRRRR"
     gene            17613..18287
                     /locus_tag="VMZ53_32740"
     CDS             17613..18287
                     /locus_tag="VMZ53_32740"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUS33332.1"
                     /translation="MALRLYQFAYSPYAAKVRLVLDLKGLAYDLIEVPYMDRTEVVRL
                     TNQVVVPVLTDDRGGSSAESRIAIHDSPRITAYLDEHYAPNLRPTAAAVVFEQWADST
                     FEDVAFRIASPMVEPRMAALNGGRIDAAGMYRFVKERKFGAGCIDQWARDTPALTARL
                     RDLAAPLARTLTGQPYLLGAEPTLADAAVWGNLYMLESVAAGWVARELPELAAWYARL
                     AERTRR"
     gene            18350..18808
                     /locus_tag="VMZ53_32745"
     CDS             18350..18808
                     /locus_tag="VMZ53_32745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33333.1"
                     /translation="MRMLSALLVVALASGCSAPQAHRVRRPSELAIGGSLVGVMASSL
                     AIAALPGQKHVIIPIAIGFGGLAVVSAVVFGVAYANDPPPEPAPLPPPPPDHRAEAWA
                     LTKQAQDAARAGDCTTVATLDAQVAQIDASFHAVVFARDVAIARCLQSRH"
BASE COUNT         3077 a         6353 c         6138 g         3271 t
ORIGIN      
        1 gacttcacgg caaggacgag cgcgtcgcgg tgagcgcatt cttcgagggg cagcagtatc
       61 tgtccgagct cgtgaaagcg atcgccggcg gtaagtgatc agcgatcggg accttcgcgc
      121 cacgtctcga cgagaaagtc gacgagatgc ccgcggtcga cggccacata cgtcgccgca
      181 ccgaccgcga gcatcgcgca caacgcatgc gcgaccgcgc gcttcagcgt cacccgcgta
      241 gcagcgagca gcgcgcccgt cacccagctc gtcacgacgc cagcgatggc gatgccccac
      301 accgcgcgtc cgacgagcgg atttcccgtc gacgcaacgg cgataagcac acccgcaatc
      361 ggcacgaaaa agatcgcttc gacgggcggt cgcacgaggc gacgccccgc agcgcgccac
      421 gagcccgcgc ctcgccgcaa caggaccgtg gccgcgattg cgagcagcgc aaggagcgcc
      481 cacatcgccc aacgaatgtc gcgccgcaac ttcgcgcgct cgagtgcggc ccgcagattc
      541 gcaatgagcc ccgggctcgc cgcgacgttc gcacccgcgc cattcgcgcc cgcgctgttc
      601 gcagcgagcg cggctagctc ggcctcggct tgcgatatgc ggccggcgcg gatcagcacg
      661 cggacgcggt cggcgcgcac ccgcaggcgg tcgagcgggt ctattgcggc gcggtcggcg
      721 tcggcgagcc acgcgagtgc gcgatctgcg tcgccttcgc gctcccaccc cgcggcgatc
      781 tgcaccatca gcattgcgtt gcgcgggtag ccggggtgct cgcgcgcgag cttgccgaga
      841 gcgtcgagtg cggcgcgcgg atcacccggg ctctgcagtg cgggcaacag gcgctcttcc
      901 tcggcgatca ccgcgcccca ctcgccactc tgccctgcca cgcggctgag tcgagcaagg
      961 tcggcttgcg cacgctgcac gaggatcgag tcaccggacg cgccggtcgt gctcgtcgcg
     1021 ccgctgccga tcgatgcgtt gcctcgtgcg cgggccagcg cgcgactcgt ttcgattgcc
     1081 ttctcgaggt cgcggcgggc acgcgcgtag tcggcgttcg cctcggcgat gcgcgcggcc
     1141 tcgacccagg cgtcgtcggc ccacatcgac gatgtcgcgc cgatgcgctc gagcgcgtcg
     1201 atgtcgcggc gcgtggacgc atcacgaaag tccgccgcgt catctgcgcg agcgatcgat
     1261 ggcacgagcg gcacgagcgc cgccagcgcc acgagccacg cgacgagcgc cgaggccggc
     1321 gcgctagagg tgcgtcttcg aatatgcgcc actcgggacc gcggtgccgt cggcgcggaa
     1381 cgtctccatc gtgagcgtgt ctttgcgcgc gcgaatgacg gcggcggtgt gcacactctc
     1441 gacgtactgc gaccacacgg caggcgtacc gaagccgtag agctcggcgc ctgcgccacc
     1501 tgcgacgacg tagaccgtgc cttgctggtt cgacgtgacg acttgcccgc cgaacagcgg
     1561 cttcgtcatc tcgaacatgt gctcgtgacc gttcagcacg agatcgagct tgtgcttgtc
     1621 gacgatcggg ccgaacgcgg cctgcagtgc ggggtccgag ccgtggcgcg tgcccgacga
     1681 ccactggcag cggtggtgca tcatcatgcg ccagcgcgca tcggtgtgcg cggcgaagtc
     1741 ggcgtccatc gcttgcggga tgacgcccgt gatgtcgctc gggttatccg gagagtcgtt
     1801 gccgacgaag atgcgcgaga agccgtacgt gaagccgaag ttctcttggt cgcctggcat
     1861 cgcgaactgc gagtagaagt tgatcgcgtt cgcttcgtgg ttgccgttgg tgagcacgac
     1921 aggcgtgctc gcgaacagtg gttcggcagc ggcttggaac tcttcccact cgggttgcgc
     1981 gagaccgatc gtcgtcgcgt cgccggagaa caggacgagg tccggcatgc gttgctggat
     2041 ttgtgtcgcg agctgcgccc agacgtcgta gccaccgcgg ctgtcgccga cgtagccgaa
     2101 gacggtttcg gcgtcgggat gcgcggtcac gtcgggcgcg gtgtgaaacg tgtagaccgg
     2161 cgaccacgaa tcgccggtgc cgacttggta gctgtacgtc gtgccggcct cgaggctgca
     2221 caggtgggct tggtgcacca tgtacatcgc cgagccggtg ccctcgtagc ggaagcggac
     2281 gcccttggtc tcgttcgtca gctgcgcggc cggcaggttt gcgccttgcg cccagcggat
     2341 cgagccagcg gtcgtcgtgt cgtccatcgt ccgccactgc gcgacgatcg atgtttttgg
     2401 atcaccgacg atgccgaggt gaacgagccg cggcgtcggg tccatgccga tgatcttgcc
     2461 ggagaccttc ggtggttccg cgccgatgcg cgtcgtgatc gagtagccgc agccgtcgat
     2521 ttcgagggtt ccaccggtgt cggtgaacgt ggtgtcctcc tcgtcatcat cgcaggcgga
     2581 cacgagcaac agcagtgaga gcagcgggag cgcgcgcatc tagccttttg taacagcaag
     2641 ctgggggtat ggtgttgcag atggtgcagt cctgcctgtt gcttgcgctc gcgatcacag
     2701 tcgcggcctg cggtggcaag gcaaatcccg caccgggtgg cgacgacgat ggcagcgcgg
     2761 acgacggcgt gttcgtcaat caggctgggt tcgaaccgac cgcctttacc gtcgaggtca
     2821 gcggcgaagg ccgtccgatc atcttcattc cgggcctcgg ctgtccgggc gaaatgtgga
     2881 acgacgtcgt cgagaagctg agcgatcgct acgagagcca cgtgctcacg ctcgcggggt
     2941 tcgcgggcaa cgatccgatc aagccgccgc tcggcgccaa ggtccgaaaa gagctgatcc
     3001 gatacatccg ctcgcacaag ctcgagtccg cgatcgtcgt cggtcacagc tacggcgggt
     3061 tcatcgcgta ctggctcgcg gagaccgcgc cgcgcgacgt gcttggcgga gttgttatcg
     3121 tcgatgcggg gcccggcggc gaagcgaacg atgaagaagc gcgcctgctc cgtaacgcgt
     3181 gggcgcaagc tggcgacgac gagctgcctc gccagatccg cgcgaagttc tcgatgatga
     3241 cggcgtcacc gcagaagatg gagccgttca tcgccgagat cgcgaagtca gatcgccaga
     3301 cgatcggcga cgcgatcttc gaatacgtaa agaccgacat caaagacgac gtcgagcgca
     3361 tcaaagcgcc cgcactgatc gtccttgccg acggcggcct gcaagggcgc taccgcgccg
     3421 aggcgtccga cattcccgat cacgaggtcg tcgtgctgaa aggcgcgaag cacttcgtgt
     3481 ggttcgacga tcccgacggt ttcatcaacg ccctcgacac gttcatccgc cgtaccgatg
     3541 agcacgactc ctccgccagc cgctaaggcc aacctcgagc gcaagcggct tccctggcga
     3601 agcctctcgc tgttcgcgat cgccgtgctc gcgacggtcc tcttcgtatg gatcgccagc
     3661 gagctgcgcg agggtgagct cgacaagatc gacacgcgca tcgcgatcgc ggtccaccac
     3721 gatcagaacg tcgtcctcga tgtgctcgcg atcgcgttca cgctgctcgg ctcggggccg
     3781 atcctgcttt ccgccgtcgc cgccgtttgc ttctgggcgt atcgccgcgg tcgtcgcagc
     3841 tacatcgtga tcgtgatcgc gaactgcttt atcgcgatgg tcctgaaccc gctgctcaag
     3901 ctggtcttcg agcgcgcgcg tccgacgctg ttcgaggtca tctcgcggcc cgacacgtat
     3961 agcttcccga gcggccactc gatgtcggcc gtcaccgtct acggcgcgct cgctgcggtc
     4021 atcatcgcgc tgcgtccgac ggtgaagcca atcgcggtga tcgtcgcgat gatcttcatc
     4081 gtcggcatcg ggctctcgcg cgtgtacctc ggcgcgcact ggcccatgga cgtgatcgcc
     4141 ggctgggcgt ctggcgtgcc gttcctcgtg acgaccgtct acttgctcca ccgcgcgaaa
     4201 cgcggcgaag cgaaatagct aaccgccgcc gaatacttcg cgcgctcgcg ccgcgaggcc
     4261 ccacgcgacg ctggtcagct cttcgccgcc gacgaccttg tcctcgccga agcgcgacgt
     4321 cagccgcccg cgcaccgcag gcacgagcga gctaccgccc gtcgcgaaca cgcgatcgac
     4381 agcgctcgcc gccacgcccg ccttcgccag cacgtcatcg agcacgcgat cgatcgcgtc
     4441 gaggtcctcg tcgatccacg agtcgaagtc cgcgcgagtg accggcacgt cgagcgtgat
     4501 ctgagcgagt gcgaccttcg cagcatcctg cttcgatagc tcgaccttcg cgccttcgac
     4561 cgagcgatgc agcacgaggc cgaggtcttc ctcgacgaca ctgacgagcc ggccgatctt
     4621 cacgggatcg cgcgttccgc tcgcgacgcg atcgagcagc ctctgcgtcg agtcctcctt
     4681 caagaacgag agcaagtgcc aacgccgcag cgagctgtag atccacgctg ggacctgcgc
     4741 ttcgccgcca aagccgtcga cggtgtagag cgtgccgcgg ccgagcgccg gcgcgacgac
     4801 ggcgtcgatg acgcgcgcgt cgaacgcatc gccggagatc cccacaccgc ccgtcgcgag
     4861 cacgcgcgct ttgccagcgc cgacgcggat cgccgagaag tccgtcgtgc caccgccgta
     4921 gtcggcgacg acgatcagct cttcgtggtc gagcttcgct gcgtagcgcg cggcggcgcc
     4981 gaccggctcg tactcgaaca cgacgtcggt gaagcccgcc tttgcgagcg cgtccttcat
     5041 gcgcccgacc gcgcgtgcgt catcctcttc gtcgtcggcg ccccaatagc ggactggtct
     5101 accgacgacg cagcgcgaac ctagatcgac agacgacgcc gcacggatct gccgcaggta
     5161 cgccgcgatc atgtcgtcga gcgcccaccg ccggccgaag atcgttgtct tgctgaacga
     5221 ttcgctcgcg aggtggctct tgatcgactg gacgaggcgc ccctcgccgt ctgtctcgag
     5281 ataacgcgcg atcgccggcg ctccggcgct cagcttgcca cctggctcga agtacaggac
     5341 ggtgcgccag tacggcgccc ctgccagcgg cacgaggttg acgctgccgc tctcgtccgc
     5401 gatcgcgacg gcggagttgg tcgtgccaaa atcgataccg acgtacagac ggctagatta
     5461 catcgatctg gtagcgaagc ccgaggacga gctggtcgta ggtggtcgtc tcagcgcggc
     5521 ccgtcgcttg gaagtcgctg cggaggaaga cgtcgacgac gttcgcgcgc ggattcttct
     5581 tcggaatgat gtgcagcgtg acttcgggac cgatgaagcc gcggatgcgg tcgtcgcagt
     5641 cagtccctcg acacgctcgg ccaaacgacg cggacagcga gaagctgagc ttccagatgc
     5701 cgacgccaac ccgcgtccgc ccgagccacc gctccgcacc gaaccggcgg tccgcttcga
     5761 acgcgaccgt gtagcccgga cgctccagcg tcttggcgcg cgtgttcgcg aggccgagga
     5821 cttcggcgcg gacgtctgcg tagacctgcc gcgcgatctg ctcggcggaa accggatcga
     5881 tcggccggcc ttggaacgcg gccttgatgc gatccttcgc cacagccttt agccgctcga
     5941 tgatgagcgc cttgatggtc tcggtgtcgg ggacgatcgg caccttgaac gtctggagct
     6001 gaagaccgac cgagaacgcg gcgtcgaact ctcccggcgt tagatacgcc gcgctccaca
     6061 cgccgaccgt cgggccgatc gcgactgcgc ggcgtgcgcg attgaacgtg ccgcgagcga
     6121 ccgtctcgat cgtgggcatc tcggcctgca gcttgccgat cgcctgatcg atcagcgcgt
     6181 cgtcattctg cggcggcggc ggctggtcag gcggcatcgg tgccggcatg ccctgcgggc
     6241 cataaggcgg gggctgctgc tgctccggtg gaggcggcgg catgtcccct gaaggcgtcg
     6301 cgcctggttg tgcgtacgca gttcctgcca gcaaggccag caggacaatg gaggctcgga
     6361 tcatcgagtc atcttaccga cggctcgacc agtctacttg acggggagct tctttccgcc
     6421 accggaaatg tcggcaaaga agtcatttcc tttgtcatcg accacgatga acgccgggaa
     6481 gtcgacgaca tcgatcttcc agacggcctc catgccgagc tcgggatact cgagcaccgt
     6541 cacgctcttg atgcagtcct tggcgaggcg cgcggcgggg ccgccaatcg agccgaggta
     6601 gaagccgccg tgcttcttgc acgcgtcggt cacctcgcgg ctgcggttgc cttttgccag
     6661 catcaccatg ctgccgccgt ttgcctggaa cagatcgacg tagctgtcca tccggccggc
     6721 cgtcgtcggg ccaaacgagc ccgatgcata cccctgcggc gtcttcgccg gacccgcgta
     6781 gtagacgcag tgatctttca tgtactgcgg caggccctgc cccgcgtcga tgcgctcttt
     6841 gagcttcgcg tgggcgatgt cgcgcgcaac gatcagtggg ccggtgagcg agagccgcgt
     6901 gcgaatcgga tagcgcgaga gcgtcgcgcg gatctcgttc atcggcatcg tcagatcgat
     6961 cttcacgacc tcgccgggca gctcctcgtg cgtgatgtcc ggcaggtact tcgcgggatc
     7021 ggtctcgagc tgctcgagga agacgccgtc gcgcgtgatc ttgccgagcg cctggcgatc
     7081 agcgctgcag ctcaccgcga gcgcaaccgg acagctcgcg ccgtgacgcg gcaaccgaat
     7141 cacgcgcgcg tcgtgacaga agtacttgcc gccgaactgc gcaccgatgc cggtctgccg
     7201 cgagatctcg aggaccttct gctcccactc gagatcgcga tacgcgtggc ccgcgatgct
     7261 gcctttcgtc ggcaggtgat cgaggtaccg cgcgctcgcg agcttcgcga cctttagcgt
     7321 gtactcggcg ctcgtgccgc cgatgacaac ggcgaggtga tacggcgggc acgcggcggt
     7381 gccgagcgag cgcagcttct gatcgagaaa cgcggcgagg ctcttcggat tgagcagcgc
     7441 cttcgtctct tggaacagga tgctcttgtt cgccgagccg ccgcccttcg ccatgaagag
     7501 gaagtgatac tcgtcgcccg ccgttccgta gatttcgatc tgcgcgggca agttcgtgcc
     7561 cgtgttcttc tcctcgaaca tgtcgagagg cgcgacctgc gagtagcgca agttcgtctc
     7621 ggtgtacgtc gtggagatgc cccgcgagat cgcttcctcg tcggtgccac cggtcacgac
     7681 gcgctcgccg cgcttgccca tcacgatcgc ggtgcccgtg tcctggcagc cgggcagcac
     7741 gccgcccgcc gcgatgttcg cgttcttgag cagctcgagc gcgacgaact tgtcgttcgg
     7801 cgacgcctcg ggatcgtcga ggatcttccg tagctgcgcg aggtgaccag gccgcaagag
     7861 gtgcgcgatg tctcgcatcg ccgtctgcgc caggagcgtg agcgcctccg gctgcacatc
     7921 gaggaacgat ttgcccttcg cctcgaacgt cgagacgaag tcgcccgtca gcttcttgaa
     7981 cggagtgtca tcgtgatggc caaccggcaa cagctcttga tacgcgaacg tcggcatggc
     8041 gcgcagcata gcagccgccg tcgacgacga cgctagtcgc ggtagccgac gacgctagtg
     8101 acgcagccgc acgattccgc cgctctcgac ggcccaccgc cgcggcgtcg cccacggatc
     8161 gccggtcacg cgcggctcga aactattcgc atgatagatg ccggcctcga cgcccatcgg
     8221 cagcttccag aaggcctcga cctccatccc gtaacggacg agcgtctcga cctgggtcgg
     8281 cccgccctcc atcggcttca gccacgagat acctgcgcgc gcgccaaaca gcatgctgtc
     8341 gctctcgatc gagctcgcga gctcgaagcg aacctcggcg aggatgcgtt gagcatcggg
     8401 cgtcgtcgtc gggttgcgcg agatgttcac cccgaggccg cgcgtatcgc cgtcgctctt
     8461 gggcttccac ttgagcgccg tcgcaaagcg catgttgaac aggctgtcgt taatcgtgcc
     8521 ggctgcattc tcagcctcga cccacgagcc gccgagatac gcgtcgaaga ccgcgaacat
     8581 ctcgtgatcg atgtagagcg tcatgtcctt cgcgccgacg agcacatcga tatgacgcgt
     8641 ctccgcgccg ccattgcccg gtccgatcgg ttgcgtcata tcgatcggca tcacgcccgg
     8701 ccggaagcgg acacgctggc cttcgatgtt cagccgtacg atgtcgatgc gtccgcgcgg
     8761 cagctcgccg tctgcaggtg cagcggcgat gccgatcatc gtcgtgttgt gattctcgga
     8821 gtcgagcgtc ggtgcatcgt gcgtttccca cgccgtcccg ccgtagctgg cgccggcaac
     8881 gatcaccatt tgcaggtcgc tcttcttctt gccgaggggc accagcactt ccaccgccgc
     8941 ctcgccgctc gcacgttcgc tcggtcccgt ccgcaccggc gcgagcgcgc gcgcatcgcc
     9001 atgctcggcg ttcgccgaaa ccgtttccac gagaccgaag tcgccgccga tcagaatgcg
     9061 gccctgaaca agcccgcgat gccgtccgcg cacgccgcga cccgacaaac catacagctc
     9121 gctgcgccac gcagcggtga gcgaatcgct gtggtacccg agcccgagcg cgcccgagcc
     9181 catgaatgcc gcgcgcgcat cgccgtcggt ccgccccgtc tgcggatcga aggtcccgcc
     9241 gtcatcggcc cgcgcaccgg cgatgccgat gaagtacgta cgccccgact tcgtgcgctc
     9301 gtcgtatggc acatcgcgat cgaggtgaaa cgcgggctcg aggagcggaa cgtcgggcag
     9361 ctgcaagtcg aggcgctcgt cggcacgcgc gtcgcgaccg ccgaggtgcg ccccagcgac
     9421 ggcgacgagc aaagcagcgc gaaacgcccg catccagcta caagatgacg cccgaggcgc
     9481 cggccagtcg catgaaatcg actaacgatg agccacgccc cgcgatgtcg cagtggcaac
     9541 ctgcggcaga gctacctacg gcggtggcgc tcggcgtgtc gcccgcgtcc ccgcaagtcg
     9601 tcgcgatgtt acgcgagcga cgcgacggcg atggccgcgg cgaagccgag cgcgaacaac
     9661 acgagcgccc acagcgcaac gcgcgccatc gagatgctgg tcggttggtt cacgatcggc
     9721 gtggtgcccg tcgccatgcg cgaaggcgcc gtcggcagct cgccacttgg cgcgaagctc
     9781 ggcgcgggcg gcagcgccat atcgctcggc gtcggcctgc cctgccagac gcgatgtgcg
     9841 ggtggtgtga acgcgtcgtg gctcgcgcgc cccatgtcgc tacccgcgcg ccccatgtcg
     9901 ctacccgcgc gtgcgtgacc ttgcggcggc gtgacgatgc gaacgacggg gacgcgcgat
     9961 cggttggcga ggcgttcgat ctccgcacgc gccgacgcgg tattcgtcga cgtgccgctc
    10021 ggcggcgtga ggacgcggcg cggctgacgc gcggtctgcg cctgcgagtc ggcattcgta
    10081 gagctgcgcg catcgatgtg cagatgcggg ctcggagcgc gtgggctcgg agcgcgtggg
    10141 ctcggcgcgc gtgggctcgg cgcgcgaggc gcgtcgggcg acatcgtcga tgcgagcgga
    10201 tcgatcgcct tgacacctgc ggacggcgac atcgtcgacg ccatcggatc gacaggcgtc
    10261 acattgcgtg caggagcgcg cggcggcggc ggtggaacat gcgcgggcgt tacgcggcgc
    10321 ggcgtcacat atggattgct cgccggcggg atctccgtgc gagagcccac ggcgaaccgc
    10381 tgcgagggcg cgcgaacctg tccggtgcct tctgcatcga acgcggggcg cgtcggcggc
    10441 agaccgatcg ttgtcgattg cggcggcgcg ccgacccacg tgtcgccatc gtcgtcgggg
    10501 aacgcgccgt catcggactc gtccctgaga gcttcatcga taagcgcgtt gatctccgac
    10561 gatgcatccg acgtggcgtc cgacgacgca cgaggcgcga cgaatggctt cgtgccttcg
    10621 atgagctgga cgagcacgtt gtcgagctct tccatgtcgg gccgctcgtc gatctcgtgc
    10681 gacagcgcgc gcatgatgat cgcgtcgagg tgcggcggca cgttcgcgcg ctccgcgagt
    10741 ggaaccggcg gcgtcgtgag catcgccatc aagcggctcg cgggaccttg cgcgggcgaa
    10801 aacggcagct gccccgcgat catctcgtac atgacgacgg cgagcgcgaa cacgtcgctc
    10861 ttgccggagc acgcgccacc gatgagctgc tcgggcggca tgtaatcgaa tgtgccgatc
    10921 atctgaccgg cgagcgtgag atcgggcagc gcggcggtct ctgcatcgac gagcttcgcg
    10981 atgccgaagt cgaggacttt ggcctgatct tgctgcagcg cggagacctc gagatgaatg
    11041 ttcgcgggct tcagatcgcg atggatgatg ccgaccgcgt gcgcttcacg cagcgagctg
    11101 cagacgccgt gtgcgatctg cgccatgcgt cgccacggca gcgggccggt gttgaggaag
    11161 tgctggtaga gcgtctcgcc gcgcagcagc tcgagcacga tgtaaggcgt gccatcggcg
    11221 tccgcgccga cgtcgtacat cgtgaccgtg tgcggatcgc gcagtcgtgc gagcgcctcg
    11281 gcttcgcgac gaaagcgtgc gacgagcagc gcatcactcg cgagctcgcg atgcagcacc
    11341 ttgatcgcga cttcgcgtcc catgatgagg tcggtggcgg catagatcgc accgaagcca
    11401 cccgcggcga gcttgcgctc gatccggtaa cggccgtcga cgacgtcttg ctccctcatg
    11461 ccttctacaa acggacatga cggcgcttgt atttattttg cttttgcgag gagctgcggc
    11521 cagttccggc cgatcggcac tgggaatatt ttgcgacagc ctacgtatcg aaagctcaca
    11581 gtggctcgag cttcgccaca tcgccaggag ttgccgtgcg cagccacagc aagtactcgg
    11641 cgagcgcgtc acgatcgatg atcgagagat ccttgtcgaa gcgcggcatc tcgctgtgct
    11701 cggggcccat gtgcaccggc gacttcggat tgccgatgaa gtgcgtgtac cagtcgcgac
    11761 tgccgaggcc accgagaccg gggcccgacc cacccgcgac gccttcgtcg aggctgtggc
    11821 agtcggtgca cgctttctcg aacacgccgc gcccgcgctc gcgccgtgcc ggaatgacgt
    11881 ccgtcgcgcc gctctcggcg tagatcgctt cgacgagatc gtcgagctcg gcaccctgta
    11941 gatcgacggg cttcatcgcg tcctcggtct tcgcgagctt cgtcttgccc cagtaggcag
    12001 cgccgttcgg atctttcagg aagcctttga cccagtcgcg cgagccgtga cccgccgcga
    12061 tgatgggtcc cttgcgatct ttgctgtcgg cgtcgtggca ctccgcacag cgctgcgccc
    12121 acagcgtacg cgcgcgatac atcggaatcg tcgtgcgaag atccgcgggg cccgtcacgg
    12181 gcacgccttt catcgcggcg agtgcgcgca agcgtccggc tttgacttct tcttcgtgct
    12241 gacgcttcgc gagggccttg tcgctggagt catgcatgaa cgagctcatc gtcaacagcc
    12301 cgatcaccgc gaacaacgcg acggtcgcac cgacccacac gaggcgcttg cccggtgcgc
    12361 gcgtgttgct gcgatcgagc agcggcaaga gcacgagcaa cccgacgacg atcgccggcg
    12421 cgatcatcgc gacaatctgc tcggccgatc cggcaatcga gcgcagcgca tacagccagc
    12481 ggaaatacca aagcggtcgc gcgtcatacg ccgccgacgg atccgcaggc gccgcgagat
    12541 ccgcaccatg cgtaaagttt gtccagaaca cgagcaccgc gatcacgagc atcatcgcgg
    12601 cgacattcaa gaagctctga tgcggccacc gcctgaccgg cgcctcgtcc ggatcgcgct
    12661 tccagtgcgc cgtcgcgcca tgccgcctcg cgaccacgat gtggccgatc gtcagcaccg
    12721 tcacgatcgc cggcagcaac acgacgtgca gcatgtaaaa ccgcgtcagc gtcaggttgc
    12781 cgtactcgtt accgccgatg aggaacttgc gaatcacgcc gccgaggacc ggcgtgcccg
    12841 ccgcaatgcc gacctcgact tgattcgccc agaagccagc ctgatcccaa cgcagcacat
    12901 accccgtgat cgtgaagccg agcacgagca gcatgaggaa gacgccgagc caccacgtca
    12961 gctcgcgcgg ccgcttgtac gcgccgaaga tcgcggcctg cagaagatga atgcccgaca
    13021 cgatgacgag cgcgctcgcc gcatgcacgt gcatgccacg cacgaaccaa cccgcggtca
    13081 ttcgatcctg cacgtacgcg acggacgccc acgcatccgt cgacgacggc gaatagaacg
    13141 ccgcgagtgc cgcgccagta atcagctcga cgagaaacaa aaggacgagc gtccagccaa
    13201 acgtgtacgc gaacgacacg cccggaacga cggacggcgc ccgctcaccg cggcctagcc
    13261 gctcggcaaa ccacgcggcg acgccgcgcc tgcgtggcgg cgtgcttccc tcgcgccgct
    13321 cgctactcgc tgcggccggc ctgcccggca ctgcgggccg cgatgacgcc actgctggcc
    13381 tcgatgacgc cgccgctggc ctcgatgccg ccactgcgct cgcagatggt gctgcgctcg
    13441 ccgcgatcgc gcttgccgat ggcccgccgc tcgtcgatga cccgccgctc gtcgatgacg
    13501 cgccgcttgt cgatgacccg ccgcttggcg atgacgcgcc gcttggcgat gccccgccgc
    13561 ttgtcgatga cccgccgctc gtcgatgacc cgccgctcgt cgatgacccg ccgcttggcg
    13621 atgacgcgcc gcttgtcgat aacccgctcg atgtagctgc tgctgccgcg agcgtggctt
    13681 tgcccgcgag ccctgcagcc tgtgtccggt tttcggacgc acccgtcgcc aggccgcttg
    13741 cgcgcgatgc tccggtcgtc gatgttccgg tcgtcgatgg tccggtcgtc gatggtccgc
    13801 tcgtcgatgg cccggtcgtc gatggtccgc tcgtcgatgc tccgctcgtc gatggtccgc
    13861 tcgtcggggg cggcgacgcg gtgatcgacg gttcggtcgt cgtgtcgctg gtcgcgggct
    13921 cgagcgcggg tccactcgcg agcgacggat cggtggtcgg aacgccggac gacggctcga
    13981 cggcgagccc agaggttcgc attccttcgg gcggcgttgc ggtacccggg ggtcgcttga
    14041 tcgcggggct ggtctgcgat ttgcgctttt tcgtcccgcc gctggcaccg ccaccaccac
    14101 caccggcacc accaccacca ccaccgccac caccaccggc accaccgccg gcgccaccac
    14161 tcttcggcgt accgccactg ccaccgctgc tgcctttgcc tttgccggtc ttggtcacgc
    14221 cggctcccgc gatgcggtgt cgagcttgta gcggacccac gtcaggcgca ggcggccgtt
    14281 ctccaccttg atcggcagcg ggtcgaggcc gcgcagcgat ggaccggttt gtttggcgcc
    14341 gtcggcggcg aacttgctgt cgtggcaggg gcagaggaac gacttgcctt cccagccgac
    14401 tgcgcagccg agatgcgggc agacgccgga gagcgcttcg aattgatcgg gcgtggaggt
    14461 cgcgcggatg tacgcggcac cgaggacgat gttgcgcgcg gtcgtccagc cgtcccgaac
    14521 ttcgggcgcg atgacgtcga ccttgcgcca gtcgctccag cctttggcga tcgtcgcgtc
    14581 gccgagatcg atcggatccg tgggcgtcgt taccgtctgg cgcgacgtcg gcgcgagcac
    14641 cagcgatacc gcgggtgcgg tcgctgcaat gccgacgatt ccgccgagcc cgcacgtcgc
    14701 gagcttgagg aacttacggc gatccggatc gtcctcactc attctcgcta cggtaacaga
    14761 gggtcaccgc gcgcttcacg tttcgcccct tatcaatagg agggcgatcg ggcgttaacc
    14821 catcgtgcgt tcttcgcgtt cctgatcctc tttgcacttg atgcagagag tggtctcggg
    14881 acgcgcttcg agtcgcttct tgccgatcgg ctcttcgcag acctcgcaga cgccgaacgt
    14941 gccgtcgtcg atcttcttga gcgcgaggtc gagcttcgac agcagcgact tctcgcggcc
    15001 gcgcaggcgg aactcgaacg actgcagata ctcgctcgac gcgagatcca tctcgtcggg
    15061 gagatcattc gtgtcgaggg ccatgttctc gacggccagc atttcgcggg cgcgctcgag
    15121 caccgcttta cgtttttctt cgaggagctc ctcaaacttc ttcagctcct tcttggtgag
    15181 cgctttcgac gaggtcgctg cagtggtgct catagggccc tcaaaaggcg cgacaggata
    15241 gtgacgaccc tctaccgtgg caatgggtca tcgcgagatc caccatcata cattggtgct
    15301 tcgcttgaca ccgtgtgggc tatccatcga aactaactcg cgtatggcag ggtcggacaa
    15361 gcgcaagcag tcactctact ttcccgaaga catgctgaag gaaatccagg acgaggcgac
    15421 ccggcaggac cggtctctct cgtggatcgt ccagaaagcc tggaagatcg ctaggaaaga
    15481 gataatgaag tacccctcgg tcaacgagtt cccgggcgaa gaagacgaga aagagatcgc
    15541 ctaacacgcg attggctttc tcgtggcgag cacgagatcg tggagctacg ccgtcgccag
    15601 tgacggtact ccaatatttt acgaaggctt cggggagcca gagggtcaag gcacggcggt
    15661 acgtacgccg gtgcttttgt gcgacggcat cggctgtgat ggctatgtct ggcgctacct
    15721 ccgcgaggac ctgaccgatc gattcggtct gcatcctcat tacagaggac acggccgcac
    15781 gaccgcgcca cgcgatccga cgcgggtacg gatcgaggat ctcgccgacg acatcgcggc
    15841 cgttctcgac gacgcttccg tcgaacaagc cgtactcatc ggccattcga tgggcgtgca
    15901 agtcgcgctc gagacctatc gccggcatgc agcgcgcgtc gcgggcctcg tgctcgtgtg
    15961 cggcgcgcca tcgcatcctc taaagacgtt ccgcggctcg tcgacgctcg aagagctact
    16021 cccgatgttc gagaagtgga tccatcgcgt gcccggcgtc gtgaaccgat tgaccaggac
    16081 gctcctgccc actcgcctag cgtacgaagt cgcaagtcgt ctcgagatcc gacgcgagct
    16141 cgtcgagccg agcgacttca tgccgtacct cgaaggcatg tcgcgcatcg atgcgcggct
    16201 gttcgtcgca atgctgtcgt cggcgggtca gcactccgcc gaagatctgc tcccgtcgat
    16261 caacgtcccg acgctcgtga tcgcaggaga acgagatggc ttcacgccgc ccgatcgcag
    16321 tcgcgcgatg gcgggagcga tcccgggtgc ggagctctta gagatagagg gcgcttcgca
    16381 tacggctcca atcgagcgtc cgagcctcgt tgattcgcgg attcgcgact tcatccgagg
    16441 ccgcgtcgat gcgccgaccg accgtggaat ttcgccccag tcctgacttt tggccggtgt
    16501 gcgctgacac gcgctggaag ccgaaacgaa cgcactagat gacgctccag ccgggatctc
    16561 gcgaaacgct gacggcgctt ccgccgatca acgctcacga gccgtgaaac cgggagtcga
    16621 ccgaatcgac tagcgttgct cgccgattta tcggctcgac tgggagcgct tttgtggcat
    16681 taccagtcgg ctcgggggac gatatttcgg agcgcgattc gttgcgcgaa cggatctggt
    16741 tctcccggga gtgagaacag gaaatatcgc ttcctgaggt cagttgaacc cgcctcgggt
    16801 tagccccagg ttatttctga atctgaatcg attatcagct tgtcgcgagt caaatttttg
    16861 agggttcgca cgcggaattt catcgctttt cgaaaactga tcaggcaagg tcgcggtcga
    16921 tcgcatcgcg tccttacggg ggaaaagtaa aggcactcga tgcgagacca ggacaaagaa
    16981 gccatggaaa ccgacaagtc aatgacacgc cctgtcctcc gtcggcccga tgacgacaat
    17041 gatggtttgg ttgctttgcc gcccgtcccg ctggttcgcc agcggctccg cgcgtcggtg
    17101 aaggaatttg ccacgtcgca accgggtcgt cgtgccgctg ccctcgccgc cgtgtggatc
    17161 gcagccaccg gatgtgaggc ggatctcaat cattacgatc ccgaagaagc ccttcggacg
    17221 tatcgcctca tcgagtccga gctacgcgcc gagctgcgca tcagcctcgg tcgcgcgatc
    17281 acgaacgagc cgcatgcggc tacgcgcaac acgatgatca gcatgctcga gcacctcgag
    17341 gagctcgaag ctgccgcggt tgcaccgcgc ccggcgcgcc gtcgtcgccg ccgctagtta
    17401 gtacattcgt ctgtaactgc gagggagctg agccaggggg ctcggctctt tttcgtttcg
    17461 gccgacgcga atctcgattc ggcgttcacc gcgcggagat ctcgttcgca cgcgtaccga
    17521 agtaacgtga accggtctcg atagctggcc cggagatcgc gcgaccgatc tgtaccgcct
    17581 gcgccgcggt caatcgtgaa accgttcgca ccatggcact caggctctac cagttcgcgt
    17641 acagcccata cgccgcgaag gtgcggctcg tcctcgacct caaaggtctg gcctacgacc
    17701 tgatcgaggt cccgtacatg gatcgaaccg aggtcgtgcg gctgacgaat caagtcgtcg
    17761 tcccggtcct gaccgacgat cgcggcggct ccagcgctga atcgcgaatc gcgattcacg
    17821 acagccctcg cattaccgcc tatctcgacg agcactacgc gccgaacctc cgccccactg
    17881 ccgccgccgt cgtgttcgag caatgggccg actcgacgtt cgaagacgtc gcctttcgaa
    17941 tcgcgtcgcc gatggtcgag ccgcgaatgg cggcgctcaa cggcggccgc atcgacgccg
    18001 ccggcatgta ccggttcgtc aaagagcgaa agttcggcgc gggctgcatc gaccaatggg
    18061 ctcgcgacac gccggccctc accgcacgcc tccgcgatct cgcggcgccg ctcgcgcgga
    18121 cgctgaccgg ccagccctac ctgctcggcg ccgagccgac gctcgccgat gcggcggtgt
    18181 ggggcaattt atatatgctc gagtccgtcg ccgccggctg ggttgcgcgc gagctgcccg
    18241 agctggctgc gtggtacgcg cgcctcgccg agcgcacgcg ccgttagctc gcttcgcctg
    18301 aacacacgcg cgattaactt accccggtca tcgcgcgata ctcgggccga tgcgcatgct
    18361 gtcagcgttg ctggtcgtcg cccttgcgag cgggtgcagc gcaccgcagg cgcaccgcgt
    18421 gcggcggccg agtgagctcg cgatcggcgg ctcgctggtc ggcgtgatgg cgagctcgct
    18481 cgcgatcgcg gcgctgcccg gacagaagca cgtgatcatt ccgatcgcga tcggcttcgg
    18541 cggcctcgcc gtcgtcagcg cggtcgtctt cggcgtcgcg tacgcgaacg atccgccgcc
    18601 agagcccgcg ccgctgccgc caccaccgcc cgatcatcgt gccgaagcgt gggcgctcac
    18661 gaagcaagcg caggacgccg cgcgcgccgg cgactgcacg accgtcgcga cgctcgacgc
    18721 gcaagtcgcc cagatcgatg cgagctttca cgctgtcgtg ttcgcgcggg atgtcgcgat
    18781 cgcgcgctgc ctgcaatcgc ggcactgatc gcatcgcggt cgtcgcgcgc agagtgtcc
//
LOCUS       DATLBZ010000665        16136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6479150, whole genome shotgun sequence.
ACCESSION   DATLBZ010000665 DATLBZ010000000
VERSION     DATLBZ010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16136
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6479150"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VMZ53_32750"
     CDS             complement(<1..542)
                     /locus_tag="VMZ53_32750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUS33334.1"
                     /translation="MARFAWEGTTRAGEKRRGVIEADSRAVVEERLRSDGVMIKSVKK
                     EGGLGDIQIQIGSGVSAKDLQIFTRQLATMIDAGLPLVQCLDILAAQSENKSFKKILT
                     SVKNSVEQGATFSDALKKHPRVFDELYVNLVQAGEIGGILDTILNRLAIYIEKAVKLK
                     NQVKSAMYYPIGIMVVAIGV"
     gene            complement(554..1678)
                     /locus_tag="VMZ53_32755"
     CDS             complement(554..1678)
                     /locus_tag="VMZ53_32755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HUS33335.1"
                     /translation="MAQLTLQALLKAMLDKGASDLHITAGSPPRLRIDNELVRLQTDA
                     LNPVDTKTLCYSVMNDAQKLRFEEDLEIDFSFGIRGLARFRANVYMQQSCVAGAFRLV
                     PYTIIPLEDIGLPPVVTDLSDKPRGLVLVCGPTGSGKSTTLASMIDRINTQQRGHIIT
                     VEDPIEFQHSHKSCLINQREIGRDSQSFKRALKYILRQDPDVVLVGEMRDLETIEAAL
                     TIAETGHLCFGTLHTNSAIQSINRIIDVFPSHQQTQVRAVLSFVLEGVVCQALIAKAN
                     GAGRVLASEVMVPNAAIRNLIREDKLHQIYSQMQIGQSKFGMQTFNQSLCDLYLRKQI
                     TLEECLGHSSEVDELKTMILNGGGSLGSYGGPQVNAPTRR"
     gene            complement(1684..3378)
                     /gene="pilB"
                     /locus_tag="VMZ53_32760"
     CDS             complement(1684..3378)
                     /gene="pilB"
                     /locus_tag="VMZ53_32760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HUS33336.1"
                     /translation="MSRIGELLVREKMLSLQQLQQAQDEAKRTGKRLGATLARLGYVD
                     DQRLTQFVAKQYSLPSINLNEVEIDATVLKLVPREICEKHQVIPVRRNGPTLVVAMAD
                     PSNIYAIDELKFLTQYNIEPVVASEQGVEAALSRYYDKGPDLDQMVGELDVDNVDYAA
                     VTDENVNVVDLENQAGEAPVVKLCNAILLSAIKKKASDIHVEPYEKSLRVRFRIDGIL
                     QEEMRPPLKLRNAITSRLKIMASLDIAERRLPQDGRIKLKIGGNREMDFRVSVLPTLY
                     GEKIVMRLLDKTSLQLDMTKLGFDIGPLDHFKEAIKKPYGMVLVTGPTGSGKTTTLYS
                     ALSELNTIDRNISTAEDPVEFNLHGINQVQMHEDIGLNFATALRAFLRQDPNIIMVGE
                     IRDFETAEIAVKAALTGHLVLSTLHTNDAPSTVSRLLNMGIEPFLVTASVNLVVAQRL
                     ARRICQDCKIPAEKHPDALTKMGMSEEQIRNAKIMKGRGCGTCNNTGYKGRVALYEVM
                     PFTDPLKELVLQGASAAEIKAEMIRGGTLSLRLAGIQKIIEGMTTPEEVLRTTVED"
     gene            3583..3771
                     /locus_tag="VMZ53_32765"
     CDS             3583..3771
                     /locus_tag="VMZ53_32765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33337.1"
                     /translation="MSYAEAKQIALLRVELRTCWENQDYAGAGVALARLTAVAVGAEN
                     DNELNAEVKRWAFKLQVA"
     gene            complement(3794..4402)
                     /locus_tag="VMZ53_32770"
     CDS             complement(3794..4402)
                     /locus_tag="VMZ53_32770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33338.1"
                     /translation="MRTLRFSILSLLAAAGACTPYNPDLGESPFLCGPMEQSPRCPDG
                     YACVTMNMKDYCVAEGGSVPVDARPANCADDSQLEPNDTIQQAFQTPVATQKNNLTFA
                     GLSVCPAGDKDTYSITITTANQNLEMLIEYDDGGAELQGSILNSGGVPIANASAVSGM
                     NRVKRAYTPNLPVGTYYAQAYGPNSGSQTTNNYKLTVNVTGP"
     gene            complement(4414..5835)
                     /locus_tag="VMZ53_32775"
     CDS             complement(4414..5835)
                     /locus_tag="VMZ53_32775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33339.1"
                     /translation="MKRSILLCILITWFAAFIPATVAFADDAPPTKEQLEQAKKAFGE
                     GRALFEEGKLPEAIEKFKESYRLSKNPTLLYNIGFTLDQAGSKDKALFYYRKFLADAP
                     KNAPQRKEVDKRVVIIEKENLEADLNGNSTTTTTTGGGGAATTGGGGTTTAKVEEVKP
                     QVKVKPAGTYSATDFQHQMIDAAPATKPLDVTAFVPEDSGWTVTLYYRGNGDTKFFAK
                     TMKWRYKELVGRIPGAKMGGSTIQYYIEAKDQSGAVVAKSGKSTSPNLVNIDNSVPAR
                     FYPDLTDEGNGDSSAPSAQQVQRRDEEEDPFNKNKKQQEDIEPSGPIGTPETPTTPGT
                     GWTSVGSSKFEKAKWISTGVAGGMVAGAVVTFILGKKQASNLETDSTKCGQPPCQVFD
                     AEYDVKVQEAGQRYNTLNTVTTVVGVGATLVAGYFWYKQVTAKKRGELKVGSKNSSPE
                     TTWAITPTIGDDGFTGAAAALQF"
     gene            complement(6000..7406)
                     /locus_tag="VMZ53_32780"
     CDS             complement(6000..7406)
                     /locus_tag="VMZ53_32780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33340.1"
                     /translation="MMAVETNKGNGNPTATGTPGPAATLAGMPAVSANGRPKRKRPSS
                     LMAQAAELPSVENSLDEFIARANETLVDPDAFNSSKVRAAEDDARKEADALRWKAAEQ
                     QMRESQAREQSLRRQLDGLQGKLAEAEARAAVAGTGGTQDGAVADLKVRLTSYDEKLR
                     EAEARSQQLARELESAKAAQQAVSLAAASDSLSGAENDELEQRARLAEAKAAKALAAA
                     RAAAAGLTVSQADLAAIESGLVVTEAPKKGMSWGMIIAAFVVGGAICFAAAFVLMNNK
                     NANKASANTTPAANTQPQATTPTPSPAPAKVAPVVTPIEDDSAKTDSAAAKTDDSAAA
                     KTDDSAKTDDAAKAAKPDDTAKTDDAAAKKADEAAAKKAAADEAAAKKADEAAAKKAA
                     AEQAAADKAAAAQAAADARAAKAAAAKKTTTAKKTTTTTTPKTQKASGGGIADPFGDA
                     PAPKKKKASGGGGIVDPF"
     gene            complement(7484..7771)
                     /locus_tag="VMZ53_32785"
     CDS             complement(7484..7771)
                     /locus_tag="VMZ53_32785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33341.1"
                     /translation="MNDPRPLATLEPVRFGEFLLHKNLISDEQWLAALAEHWSASGRQ
                     RRPIGTTIVANGFLAKEIVEREARAFHEDIEVVEVMPRSEKNTVPLPPAFA"
     gene            7831..9087
                     /locus_tag="VMZ53_32790"
     CDS             7831..9087
                     /locus_tag="VMZ53_32790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HUS33342.1"
                     /translation="MAQPRRSPRRPVRGAPIVRKNTVRLPGDVAQRVRAGHPWVYREA
                     LGPRPIAPEPGTPIDLVDPDGEFVGRALYDADSAIALRVFVRNSDMQIDGKLVRDRVK
                     AAVSLRKRIMEWDRLDSMRLINGESDGLPGIVVERYGEYLVSQLYTAAVANLRDDLYD
                     ALGDVLAPKAIYEQRRYRSLGGDAPRQAGADLVRGSAAPVELEVKENDLTFVADVTAP
                     LSTGLFADLREGRRAVRQWAAGRRVLNLFSYTGAISVYAQAGGAAEVVAIDVAAKAHA
                     RARRNFAASGFDPEKPEHIVGDVFKVLARFVERGRTFDMVVVDPPAFASAAARGGKPW
                     SAMRDYSELITAALDVLAPGGLLAAASSTHKMTPEEFERAIADGALSAGTRLQVIDRR
                     PLPPDFPTVPGFPEGNYLKFIVAVRG"
     gene            9194..9517
                     /locus_tag="VMZ53_32795"
     CDS             9194..9517
                     /locus_tag="VMZ53_32795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33343.1"
                     /translation="MTTNFETIDLAQLSTVSGGEGEGGSGGGGFVQGVKDAWQGVKDF
                     SGGFAGGAIHGQNMRTSQMPFYSQNPSGQATKAGFETGAMFNQALGPAGRVLSLMGKS
                     APSGG"
     gene            complement(9519..10292)
                     /locus_tag="VMZ53_32800"
     CDS             complement(9519..10292)
                     /locus_tag="VMZ53_32800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33344.1"
                     /translation="MKLPDGIAKRLTALAVAGCALVLAGCGRLAFEPLSTSDATTSNG
                     DGDSGAPGLRPLHLYELNGTYADTYGGPSLVGAGGTFTANGYQFGINEGLSVDNALPA
                     SVYTIDIDFSFTDVAMWQKILDYKGLAADEGVYVYEGALQFVIAASADFVSGAPNLVA
                     DQRLRVTLTRDASAHVVGYLDRAPIAADRLTSAAPPASPSGTFDFDDTTHQAQLAGAT
                     AAFFIDDSGTTMGEASPGTVRRIAIWDVALTPAQVATLQ"
     gene            complement(10314..10817)
                     /locus_tag="VMZ53_32805"
     CDS             complement(10314..10817)
                     /locus_tag="VMZ53_32805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33345.1"
                     /translation="MNLPDGIKHAVHMGANEFVEWLSVDPSEEELENLGATYQSVGED
                     AAPPDAAKQYIADLKAWDDGDTSQSPGAKFESMMYTAGIEFARGLIAAAAGVGTVPDR
                     VKPHMAELTPLVDELKTAFAEGSFDEEKVDLAAIIWGSSEDDDDDDDDDEGDEDDEDD
                     DDDDDDD"
     gene            complement(10836..11195)
                     /locus_tag="VMZ53_32810"
     CDS             complement(10836..11195)
                     /locus_tag="VMZ53_32810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33346.1"
                     /translation="MSVPHVLDTLPTQIVQTAPAIDPKHIAAVRYELEYIGSPLALTI
                     SRIYELVGEGLVDPAIALPALAEACATLVAGMRGEVGESVLEAARYQIDTLTPMPDKP
                     PRVAAPDVPLTSLKRRS"
     gene            complement(11192..11908)
                     /locus_tag="VMZ53_32815"
     CDS             complement(11192..11908)
                     /locus_tag="VMZ53_32815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HUS33347.1"
                     /translation="MPVRPPVPAATRGPVRIELDWDALEIAVERNSPDTDSYLDLTTG
                     RVITITTGDPEAALNRQQVSENIRNFLRVEPASSREQYRWMEKFVGSVTDEALRERLI
                     ISVDGKGAFRRFKDVLLAYPAERERWFSYRAELLHWHIQQWLESHDITTTNEPPWGAV
                     QAPTELPTVGRPVVHGTEAPGEALRRQARELIETIPAIELPTAIAFMEFLKQRSAEAM
                     AKQHREAEAHDTALDNDQKS"
     gene            12035..12277
                     /gene="rpoZ"
                     /locus_tag="VMZ53_32820"
     CDS             12035..12277
                     /gene="rpoZ"
                     /locus_tag="VMZ53_32820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HUS33348.1"
                     /translation="MARVTVEDCLDKVPNRFALVVLAAERARQLSRGATAGVDCDNKP
                     AVTALREIAAGMVKFREDVRTTVTEFIVERRKAGFM"
     gene            12334..13137
                     /locus_tag="VMZ53_32825"
     CDS             12334..13137
                     /locus_tag="VMZ53_32825"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HUS33349.1"
                     /translation="MRDKTQVDVTAQQLERGQLVGALYRFGRRNASGILTITAHGSPR
                     NEVFVLRRGSVILADADVAKRALVQRLVRLVALDRVTLVFEPGTTAAPPGSQNVLSLA
                     TWARAHLEHQLDGTLADLLLRELAGMRIALKLELAPDPANCDEADRRMLAALAQPRRL
                     DQIWTIARTPRFRLLSFIHFLRAVDALDVVGVVADKSQPHRSIDPRRESARRLLGLPA
                     EADLESIKRAYRRLARELHPDLQPDLDLHERRTLERRFAEVTAAYEALL"
     gene            complement(13143..13979)
                     /locus_tag="VMZ53_32830"
     CDS             complement(13143..13979)
                     /locus_tag="VMZ53_32830"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HUS33350.1"
                     /translation="MQRAFSRFVGVDLGGGRGKTTAVAELRMGAGGTAEVIEVATRAG
                     KLPWTDDTLIGRLAIPDPNRVIAVDAPLTQTACGRCAVPVCPGTEACADPAVVWLRTT
                     GRQLMQKVAAETVGGSSRNVSPPPQARLQPYAHRATDVVMTYQRGLLPLSTLGTANSM
                     ITARAGQLRRRLRGAGYQLHGNLLEVSPAATIAALCGPRVARGYKRDADPWVTRAEVL
                     ARFDDLSFAPQSRMAREDVLRNDHCFDAVISAYTAFLWARDGWESPTGVFAEDGWIFS
                     PP"
     gene            14124..14438
                     /locus_tag="VMZ53_32835"
     CDS             14124..14438
                     /locus_tag="VMZ53_32835"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS33351.1"
                     /translation="MDRIATFRSFISKSPTDPFPRYGLAMEHKGRGELDEAWRVFEEL
                     LAQFPDYVATYLMAGGTLVALGKKAEAAEVYRKGIEVAQRRGDQHARGELESALGEIS
                     PT"
     gene            14483..14686
                     /locus_tag="VMZ53_32840"
     CDS             14483..14686
                     /locus_tag="VMZ53_32840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33352.1"
                     /translation="MKLVITFLMAAALMACGKSKKPDTTPTNTTGTTEKSTTDGATGG
                     QTYGNAGGQTTPAGGGTDPCAGM"
     gene            14758..14946
                     /locus_tag="VMZ53_32845"
     CDS             14758..14946
                     /locus_tag="VMZ53_32845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33353.1"
                     /translation="MKKIAIVLFVVAAALAGCGKKKAPAAPAGGDTGSAAPAGDTTGS
                     AAGSDAGSAAPAEGGGGM"
     gene            15042..15125
                     /locus_tag="VMZ53_32850"
     tRNA            15042..15125
                     /locus_tag="VMZ53_32850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15075..15077,aa:Leu,seq:caa)
     gene            15281..15679
                     /locus_tag="VMZ53_32855"
     CDS             15281..15679
                     /locus_tag="VMZ53_32855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33354.1"
                     /translation="MPPGDAGSCEPVACTTAGGTCVGSVCQLVATGETILSCPAGMPC
                     RLVCDGYRFCREGARCNGATWCEVVCIGFRACQAGVQCDASDCLVTCNGPEACEVGIT
                     ATGTCTAHCCGDSQTCAGGVGTCTKDTVCD"
     gene            complement(15791..15955)
                     /locus_tag="VMZ53_32860"
     CDS             complement(15791..15955)
                     /locus_tag="VMZ53_32860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33355.1"
                     /translation="MKKIAIVLFVVSAALAGCGKKKPAATPAGGDTAGSAAPAGDGSA
                     APAEGGAGGM"
BASE COUNT         2559 a         5123 c         5380 g         3074 t
ORIGIN      
        1 atgacgccga tggcgaccac catgatgccg atcgggtagt acatcgcgct cttgacctgg
       61 ttcttcagct tgacggcctt ctcgatgtag atcgcgaggc ggttcaagat cgtgtcgagg
      121 atgccgccga tttcgccagc ctgaacgagg ttgacgtaga gctcgtcgaa tacacgcgga
      181 tgcttcttca gcgcgtcgga gaacgtggcg ccttgctcga cggagttctt caccgacgtg
      241 aggatcttct tgaacgactt gttctcgctc tgcgccgcga ggatgtcgag gcactgcacg
      301 agtggcagac cggcgtcgat catcgtcgcg agctggcgcg tgaagatctg gagatctttc
      361 gcgctgacgc ccgagccgat ctggatctgg atgtcgccaa gaccgccctc cttcttgacg
      421 ctcttgatca tcacgccgtc gctgcggagg cgctcttcga cgaccgcgcg gctatccgct
      481 tcaatcacgc cacggcgctt ctcacctgcg cgtgtcgtgc cttcccaagc gaatctcgcc
      541 atgcgtcagc tcctcagcgc cgcgtgggcg cgttgacttg ggggccgccg tacgagccga
      601 gcgagccacc accgttgaga atcatcgtct tgagctcgtc gacctcgctc gagtgaccga
      661 ggcactcttc gagcgtgatc tgcttcctga ggtacaggtc gcagagcgat tggttgaacg
      721 tctgcatgcc gaacttcgac tggccgatct gcatctgcga gtagatctgg tgcagcttgt
      781 cttcgcggat gaggttgcga atggcggcgt tcggcaccat gacctcggac gcgagcacgc
      841 ggcctgcgcc gtttgctttc gcgatgagcg cctgacagac gacgccttcg agcacgaacg
      901 agagcaccgc gcgcacctgc gtctgctgat gcgacgggaa cacgtcgatg atgcggttga
      961 tgctctggat ggcgctgttc gtgtgcagcg tgccgaagca gaggtgaccg gtctcggcga
     1021 tggtcagtgc cgcctcgatc gtctcgaggt ctcgcatttc gccgacgagg acgacgtctg
     1081 ggtcctggcg aaggatgtac ttgagcgcgc gcttgaacga ttggctgtcg cggccgatct
     1141 cgcgctggtt gatcaagcac gacttgtggc tgtgctgaaa ctcgatcgga tcttcgaccg
     1201 tgatgatgtg accgcgttgc tgcgtgttga tgcgatcgat catcgacgcc agcgtcgtcg
     1261 acttgcccga gcccgtcggg ccgcacacga gcacgagacc gcgtggcttg tcggacagat
     1321 ccgtgaccac gggcggcagg ccgatatcct cgagcgggat gatcgtgtac ggcacgaggc
     1381 ggaacgcgcc tgcgacgcac gactgctgca tgtagacgtt cgcgcggaag cgcgcgaggc
     1441 cgcggatgcc gaacgagaag tcgatctcga ggtcttcttc gaagcgcagc ttctgcgcgt
     1501 cgttcatgac ggagtagcag agcgtcttcg tgtcgacggg attgagtgcg tcggtctgca
     1561 ggcgaacgag ctcgttgtcg atgcgcaaac gcggaggcga gccggccgtg atgtgcagat
     1621 ccgatgctcc tttgtcgagc atcgccttga gaagcgcttg gagagtgagc tgtgccactt
     1681 ggtttagtct tccaccgtcg tacgaagaac ttcttcgggc gtcgtcatgc cttcgatgat
     1741 cttctggatg ccggcgagac gaagcgacag cgtgccgcct cgaatcatct cggccttgat
     1801 ctcggctgcc gacgcaccct ggagcacgag ctccttgagc ggatcggtga acggcatgac
     1861 ttcgtagagc gcgacgcggc ccttgtagcc ggtgttgttg caggtgccgc agccgcgacc
     1921 cttcatgatc ttcgcgttgc gaatctgctc ctcgctcatg cccatcttcg tgagcgcgtc
     1981 gggatgcttc tccgccggga tcttgcagtc ctggcagatg cggcgcgcga gacgctgagc
     2041 gacgaccagg ttcacggacg ccgtgacgag gaacggctcg atgcccatgt tgaggagacg
     2101 cgagaccgtc gacggcgcgt cgttggtgtg gagcgtcgag agcacgaggt ggcccgtgag
     2161 cgcggccttc accgcgatct cggcggtctc gaagtcgcga atctcgccga ccatgatgat
     2221 gttcgggtcc tggcgaagga acgcgcgcag tgcggtcgcg aagttgaggc cgatgtcctc
     2281 gtgcatctgc acctggttga tgccgtgcag gttgaactcg accgggtctt cggcggtgga
     2341 gatgttgcga tcgatcgtgt tgagctcgga cagcgccgaa tagagcgtcg tcgtcttgcc
     2401 cgaacccgtc gggccggtga cgagcaccat gccgtacggc ttcttgatcg cttctttgaa
     2461 gtgatcgagc ggcccgatgt cgaagccgag cttcgtcatg tcgagctgga ggctcgtctt
     2521 gtcgaggaga cgcatgacga tcttctcgcc gtacagcgtc ggcaggacgc tcacgcggaa
     2581 gtccatctcg cggttgccgc cgatcttcag cttgatacga ccgtcctgcg gcagtcggcg
     2641 ctcggcgata tcgagcgacg ccatgatctt cagacgcgat gtgatcgcgt tgcggagctt
     2701 gagaggcggc cgcatctctt cttgcagaat gccgtcgatg cggaagcgca cgcgcaggct
     2761 cttctcgtac ggctcgacgt ggatatccga cgccttcttc ttgatcgccg acagcaggat
     2821 cgcgttgcag agcttgacga ccggcgcctc gccggcctgg ttctcgaggt cgacgacgtt
     2881 gacgttctcg tcggtgaccg cggcgtagtc gacgttgtcg acatcgagct cgccgaccat
     2941 ctggtcgagg tccgggccct tgtcgtagta ccgcgacagc gccgcctcga cgccctgctc
     3001 cgacgcgacg acaggctcga tgttgtactg cgtgagaaac ttcagctcgt cgatcgcgta
     3061 gatgttcgac gggtcggcca tcgcgacgac gagcgtcggc ccgttgcggc ggaccgggat
     3121 gacctgatgc ttctcgcaga tctcgcgcgg gacgagcttc agcaccgtcg cgtcgatctc
     3181 gacctcattg aggttgatcg acggcaggct gtactgcttg gcgacaaact gggtaaggcg
     3241 ttggtcatcg acataaccaa gacgcgccag cgttgcgccc aaccgcttac cggtgcgctt
     3301 ggcttcgtct tgcgcctgct gaagctgctg gagcgagagc atcttctcgc ggaccagcag
     3361 ctctccaata cggctcattc caagtgtctc cccaaaaaga ctcggatttt cgacagcgta
     3421 gcgtgccccc gaggcattcg tcaaacagac gggcaaaagc gaggaccgac atgtgagcgg
     3481 cgacgaggcg tcgaatgttt ttcgcgctca cacgtccgaa aacttgcggg cgatcgcaat
     3541 gccgatgaat ttgacgctgg gcacttattt aggaggcttg cgatgagcta cgccgaggcc
     3601 aagcagatcg cgcttctcag agttgagctt agaacgtgct gggagaacca ggactacgca
     3661 ggggcagggg tcgccctcgc gaggctcacc gccgtcgccg taggcgccga aaacgacaac
     3721 gagctcaacg ccgaggtgaa gcgttgggcg ttcaagctgc aggtcgcgta acgctccgct
     3781 gagcctctaa agctcaggga ccggtgacgt tgaccgtcag cttgtagttg ttcgtcgttt
     3841 gcgagcccga gttcggaccg tacgcctgcg cgtagtaggt gccgactggc aggttcggcg
     3901 tgtacgcgcg cttcacgcgg ttcatgccgg agacagcaga cgcattcgcg atcggcacgc
     3961 cacccgagtt caggatcgag ccctgtagct cggcgccgcc gtcgtcgtac tcgatcaaca
     4021 tctcgaggtt ctggttcgcc gtcgtgatcg tgatcgagta cgtgtccttg tcgccggcgg
     4081 ggcacaccga gagacccgca aacgtcaggt tgttcttctg cgtcgcaacg ggcgtctgga
     4141 acgcctgctg gatcgtgtcg ttcggctcga gctggctgtc atcggcgcag ttcgccggac
     4201 gcgcgtcgac gggcaccgag ccgccttccg cgacgcagta gtccttcatg ttcatcgtga
     4261 cgcaggcgta tccatcagga catcgcggcg actgctccat cggaccgcac aagaatggcg
     4321 attctccgag gtccgggttg tacggcgtgc acgcgcccgc agcagcgagg agcgagagaa
     4381 tggaaaagcg aagagtacgc atgacgtcgt cctctagaac tgcagagccg cagcggcccc
     4441 ggtgaaaccg tcgtcgccaa tcgttggggt gatcgcccac gtcgtctccg gcgagctgtt
     4501 cttgctgccg accttcagct caccgcgctt cttcgcagtg acctgcttgt accagaagta
     4561 tccagcgacg agcgtggcgc ccacgccgac gacggtcgtg accgtgttga gcgtgttgta
     4621 gcgctggccc gcttcctgga ccttcacgtc gtactcggca tcgaacacct ggcagggagg
     4681 ctgaccacac ttcgtcgagt ccgtctcgag gttcgacgcc tgcttcttgc cgaggatgaa
     4741 cgtgacgacc gcgccggcga ccatgccgcc cgcaacgccc gtcgagatcc acttcgcctt
     4801 ctcgaacttg ctcgagccga ccgatgtcca accggtgccg ggcgtcgtcg gcgtctcggg
     4861 cgtaccgatg gggcccgacg gctcgatgtc ctcctgctgc ttcttgttct tgttgaacgg
     4921 atcctcttcc tcgtcgcggc gctgcacttg ctgcgcgctc ggcgccgacg agtcaccgtt
     4981 gccttcgtcc gtcagatccg ggtagaagcg cgcgggcaca ctgttgtcga tgttgacgag
     5041 gttcgggctc gtcgacttgc ccgacttcgc gacgacggcg cccgactgat ccttcgcttc
     5101 gatgtagtac tggatcgtcg agccacccat cttcgcgccg gggatgcggc caacgagctc
     5161 cttgtagcgc cacttcatcg tcttcgcgaa gaacttggtg tcgccgttgc cgcggtaata
     5221 gagcgtgacg gtccagcccg agtcctcggg aacaaacgcg gtcacgtcga gcggcttcgt
     5281 cgcgggtgcg gcgtcgatca tctggtgctg gaagtcggtc gccgaatacg tgccggccgg
     5341 cttcaccttc acctgcggct tcacttcttc gaccttcgcg gtcgtcgtgc caccgccacc
     5401 ggtggtcgct gcgccgccac cgccggtcgt cgttgtggtc gtcgagttgc cgttgagatc
     5461 ggcctcgaga ttttccttct cgatgatgac aacgcgctta tcgacttctt tgcgctgcgg
     5521 cgcattcttg ggcgcgtcgg cgaggaactt ccgatagtag aagagcgcct tgtccttgct
     5581 gccggcttga tcgagcgtga agccgatgtt gtagagcagc gtcgggttct tcgagaggcg
     5641 atagctctct ttgaacttct cgatcgcttc ggggagcttg ccttcttcga acagcgcgcg
     5701 gccttcgccg aatgctttct tggcttgctc gagttgttct ttggttggcg gcgcgtcgtc
     5761 ggcaaacgcg accgtcgcgg gaatgaatgc cgcgaaccaa gtgatcagaa tgcaaagaag
     5821 aatgctgcgc ttcacagggg ccccccgcgc ggtgatacgc gtgtagcggg agggtcaata
     5881 acgcgggttt ctcgtcgttg caacgacgaa gatctgttgg ctttgcgcgg cttgcgcatt
     5941 ccgaaacgac gaacgggccg gacccaaggc ccgacccgtg ctcggtttct aagcgcgcct
     6001 cagaaggggt cgacgatgcc gccgccgccc gaagccttct tcttcttcgg ggccggggcg
     6061 tcgccgaacg ggtccgcgat gccgccacca gacgcctttt gcgtcttcgg ggtggtggtc
     6121 gtggtcgtct tcttcgcggt ggtggtcttc ttcgcggcgg cggccttggc agcgcgcgcg
     6181 tcggcggcgg cttgtgcggc ggcagccttg tcggcggcag cttgctcggc ggcagccttc
     6241 ttggcggcgg cttcgtcggc cttcttggcg gcagcttcgt cggcagcggc cttcttggca
     6301 gcagcctcgt cggccttctt ggcggcggcg tcgtcggtct tcgcggtgtc gtccggcttc
     6361 gcggccttcg cggcgtcgtc ggtcttggcg gagtcgtccg tcttcgcggc ggccgagtca
     6421 tcggtcttcg cggcggccga gtcggtcttg gcgctgtcgt cctcgatcgg cgtgacgacc
     6481 ggggcgacct ttgcgggcgc gggcgacggg gtgggcgtcg tcgcttgcgg ctgcgtgttc
     6541 gcggcgggcg tcgtgttcgc agacgccttg ttcgcgttct tattgttcat gagcacgaac
     6601 gcggcggcaa agcagatcgc accaccgacg acgaacgccg cgatgatcat gccccagctc
     6661 atgcccttct tcggcgcctc ggtgacgacg aggccactct cgatcgcggc gaggtccgcc
     6721 tgcgaaacgg tgaggccggc ggcggcagcg cgtgcagcgg cgagcgcctt cgcggccttc
     6781 gcctcggcga ggcgagcgcg ttgctcgagc tcgtcgttct cggcgccgct gagcgagtcg
     6841 ctcgcagcgg cgaggctgac cgcttgctgc gcggcctttg cggactcgag ctcgcgcgcg
     6901 agctgctggc tgcgggcttc cgcttcgcgc agcttctcgt cgtacgaggt cagacgaact
     6961 ttcagatcgg cgacagcgcc gtcttgcgtg ccacccgtac cggcgaccgc ggcgcgtgcc
     7021 tcggcttcgg cgagcttgcc ctggagacca tcgagctggc ggcggagcga ttgctcgcgc
     7081 gcttggctct cacgcatctg ctgctcggcg gccttccaac gaagggcgtc cgcttccttg
     7141 cgggcgtcgt cttcggcggc gcgaactttg gagctgttga acgcgtcggg atcgacgagc
     7201 gtttcgttgg cgcgagcgat gaactcgtcg agcgagttct cgaccgacgg cagctcggcg
     7261 gcctgcgcca tcagcgacga tggacgcttg cgcttgggcc tgccgttcgc cgagaccgcg
     7321 ggcattccag cgagcgttgc cgcaggacct ggggtcccgg tcgccgtggg attgccgtta
     7381 cctttgttcg tttcgactgc catcatggga ctccggtaag acgcggcgag agtttagcag
     7441 ctgatccgag gccccacgcc ccgaatcttc atagtcgaga cctttaagcg aaggcgggcg
     7501 gcagcggcac cgtgttcttc tcggaacgcg gcatcacctc gacaacttca atatcttcgt
     7561 gaaaggcgcg agcctcgcgc tcgacaatct ctttagcgag aaagccgttc gccacgatgg
     7621 tcgtcccgat cggcctgcgc tggcgacccg atgcactcca gtgctcggcc agggcggcga
     7681 gccactgctc gtccgagatg aggttcttat ggagaaggaa ctctccgaag cggacgggct
     7741 cgagtgtggc tagtgggcga ggatcgttca tacaggcgct attgcggcgg acactacagc
     7801 ggtgtacgag gtctgtctac cagatctttt atggcgcaac cccgcagatc tccaagaagg
     7861 ccagtgcgcg gcgcgccgat cgttcgcaag aacacggtgc gtcttccagg tgatgtcgcg
     7921 cagcgtgttc gcgcgggtca tccatgggta taccgagagg ccctcgggcc gcgtcccatc
     7981 gctcccgagc ccggtacgcc gatcgatctg gtggacccgg acggcgagtt tgtcggccgc
     8041 gcgctctacg acgccgacag cgcgatcgcg ctccgcgtgt ttgtccggaa ctcggacatg
     8101 cagatcgacg gcaagctcgt ccgcgaccgc gtgaaagctg cggtctcgct tcgcaagcgc
     8161 atcatggagt gggaccgcct cgactccatg cgcctcatca acggcgagtc cgacggcttg
     8221 ccgggcatcg tggtcgagcg ctacggcgag tacctcgtca gtcagctcta caccgcagcg
     8281 gtcgcgaacc tgcgcgacga tctctacgac gcgctcggtg acgtgctcgc gccgaaagcg
     8341 atctacgaac agcggcgcta tcgctcgctc ggtggcgacg ctccgcgcca agccggcgcc
     8401 gacctcgtcc gtggcagcgc agcgccggtc gagctcgagg tgaaggaaaa cgatctgacg
     8461 ttcgtcgccg acgtcaccgc gccgctctcg acgggactgt tcgccgatct tcgcgaaggc
     8521 cgccgcgccg tccgccagtg ggccgccggc cgccgtgtcc tcaatctctt ctcgtacacc
     8581 ggcgcgatca gcgtctacgc gcaagcaggc ggcgccgccg aagtcgtcgc gatcgatgtc
     8641 gctgccaagg cgcacgcacg cgcacgccgc aacttcgccg catcgggctt cgatccagag
     8701 aagccggagc acatcgtcgg cgacgtgttc aaagtgctcg cgcggttcgt cgagcgcggc
     8761 cgcacgttcg acatggttgt cgtcgatccg ccggcgttcg caagtgctgc tgcgcgcggt
     8821 ggcaagccgt ggagcgcgat gcgcgactac agcgagctca tcaccgcagc actcgacgtc
     8881 ctcgcgccgg gcggcctcct cgccgctgca tcatcgacgc acaagatgac gccggaagag
     8941 ttcgaacgcg cgatcgcaga cggcgcgctg agcgcgggca cgcggctaca agtgatcgat
     9001 cgccggccgc tgccgcccga tttcccgacg gtgccaggct tccccgaggg caactacctc
     9061 aagttcatcg tcgccgtgcg cgggtagtca cgccaaagac tcaggctgca taccgccgcg
     9121 agcgtgctga ccgacgcgga aacccagtgt tcgcgcactt gcagttggcg ttctggctgc
     9181 aatgagcgtg agtatgacaa caaacttcga aacgatcgat ctcgcgcagc tctcgacggt
     9241 ttccggtggc gagggcgagg gcggcagtgg cggcggcggc ttcgtccagg gcgtgaagga
     9301 cgcctggcag ggcgtgaagg acttcagcgg cggcttcgcc ggcggcgcga tccacggcca
     9361 gaacatgcgg acgagccaga tgccgttcta ttcgcagaac ccgtcgggcc aggcgaccaa
     9421 ggccggcttc gagacgggcg cgatgttcaa ccaggcgctc ggcccggcgg gcagggtctt
     9481 gagcctcatg ggcaagagcg cgccgtcggg cggctgagtc actgcagcgt ggcgacctgc
     9541 gcgggcgtga gcgcgacatc ccagatcgcg atccggcgca ccgtgcctgg cgacgcctcg
     9601 cccatcgtcg tgccgctatc gtcgatgaag aatgcggccg tcgcgccagc gagctgcgcc
     9661 tggtgcgtcg tgtcgtcgaa gtcgaacgtg ccactcggcg atgcgggcgg cgccgcactc
     9721 gtgaggcgat cagctgcaat gggcgcgcgg tcgaggtaac cgacaacgtg tgcgctcgca
     9781 tcgcgcgtca gcgtcacgcg cagtcgttga tccgcgacga ggttcggggc gccgctgaca
     9841 aaatccgcgc tcgccgcgat cacgaactgc agcgcacctt cgtagacgta gacaccttcg
     9901 tcggcagcga ggcctttgta gtcgaggatc ttctgccaca tcgcgacgtc ggtgaacgag
     9961 aagtcgatgt cgatggtgta gacgctcgcg ggcagcgcgt tgtcgacgga gagtccttcg
    10021 ttgatgccga actggtagcc gttggccgtg aacgtgccac ccgcgccgac gagcgacggg
    10081 ccgccgtacg tatcggcgta tgtgccgttc agctcgtaga gatgcagtgg ccgaagtcca
    10141 ggcgcgccgc tgtcgccgtc gccgttgctt gtcgtcgcat cgctggtcga cagcggttcg
    10201 aacgcgagac gcccgcaacc ggcgagcacg agcgcgcagc ccgcgactgc gagcgcggta
    10261 aggcgcttcg cgatgccatc gggcagcttc acgcgatcga cgataccgcg aacctagtca
    10321 tcatcgtcat cgtcatcgtc gtcctcgtcg tcctcgtcgc cctcgtcgtc gtcgtcatcg
    10381 tcatcgtcat cctcgctgga gccccagatg atcgccgcga ggtcgacttt ctcttcgtcg
    10441 aacgagccct cggcgaacgc ggtcttcagc tcgtcgacga gcggcgtgag ctcggccatg
    10501 tgcggtttga cgcgatcggg gactgttccc acgccagctg ctgccgcaat caaccctcgc
    10561 gcaaactcga tgcccgccgt gtacatcatc gattcgaact tcgcgcccgg cgactgcgac
    10621 gtatcgccgt catcccaagc tttgagatcg gcgatgtatt gttttgccgc gtcgggtggg
    10681 gcggcgtcct cgccgacgct ttgatacgtc gccccaagat tttcgagctc ttcttccgag
    10741 ggatcgacgg agagccattc gacgaactcg ttcgcgccca tgtgcacagc gtgcttgatg
    10801 ccatccggca ggttcatgcg cgcgacgtta cgccatcacg agcggcgttt cagcgacgtc
    10861 agcggcacgt cgggggcggc gacgcgcggc ggcttgtcgg gcatcggtgt gagcgtgtcg
    10921 atctgatagc gcgcggcttc gagcaccgat tcgccgactt cgccgcgcat accagcgacg
    10981 agcgtcgcgc acgcttcggc gagagcgggc agggcgattg ctggatcgac gaggccttcg
    11041 ccgacgagct cgtagatgcg gctgatcgtc agcgcgagtg gcgagccgat gtactcgagc
    11101 tcgtagcgca ccgctgcgat gtgcttcgga tcgatcgcgg gcgcggtctg cacgatctgc
    11161 gtcggcagcg tatcgaggac gtgcggcacg ctcacgactt ttgatcgtta tcgagcgccg
    11221 tgtcgtgcgc ctcggcttcg cgatgctgtt tcgccatcgc ttcggcggag cgctgcttca
    11281 agaactccat gaacgcgatc gcggtgggga gctcgatcgc gggaatcgtc tcgatgagct
    11341 cgcgagcttg gcggcgaagc gcttcgccgg gcgcctcggt accgtgcacg acggggcggc
    11401 cgacggtcgg cagctcggtg ggcgcttgca cggcgcccca cggcggctcg ttcgtcgtcg
    11461 tgatgtcgtg cgactcgagc cactgctgga tgtgccagtg cagcagctcg gcgcggtacg
    11521 agaaccagcg ctcgcgctcg gccgggtacg cgagcagcac gtccttgaag cggcggaacg
    11581 cgcccttgcc gtcgaccgag atgatgaggc gctcgcgaag ggcttcgtcc gtcacggacc
    11641 cgacgaactt ctccatccag cggtactgct cgcgcgagga ggcgggctcg acgcgcagga
    11701 agttgcggat gttctcggag acttgctggc ggttgagggc agcttcgggg tcgcccgtgg
    11761 tgatcgtgat gacccggcct gtggtcaggt ccaggtagct gtccgtgtcg ggcgaattgc
    11821 gctcgacggc gatctcgagg gcatcccaat cgagctcgat gcggacagga ccgcgggtcg
    11881 cggccggtac tggcgggcga acaggcataa gttaaggcga ttcctgccca cttgccggca
    11941 gtcaggaccg caaccctgag cgtagttggt acgggaacgt ctggcaagcg tgcccttgac
    12001 ttgcaggttc gttggggtaa gttccgcggc tcccatggcc cgagttaccg tcgaagactg
    12061 cctggacaag gtccccaacc gcttcgctct ggtcgtgctg gccgcggagc gtgcacggca
    12121 gctctcgcgc ggtgcgacgg ccggtgttga ttgcgataac aagcccgctg ttacggcgct
    12181 tcgcgagatc gccgccggca tggtgaagtt ccgcgaagat gtccgcacga cggtgaccga
    12241 gttcatcgtc gagcgtcgca aagccgggtt tatgtgacct gctggcgggg ctgaaaaatt
    12301 gccccgccgc cgcactgcgc caacgatggc gcggtgaggg acaagacgca ggttgacgtc
    12361 acggcccagc agctagagcg tgggcagctc gtcggtgcgc tgtatcgatt cggtcgacgc
    12421 aacgcgagcg gcatcctcac gatcaccgcg cacggttcgc cgcgcaatga agtgttcgtg
    12481 ctgcggcgcg gaagcgtgat cctcgccgac gcagacgttg ccaagcgcgc gctcgtccag
    12541 cggctcgtgc ggctcgtcgc gctcgatcgc gtcacgctcg tgttcgagcc agggacgacg
    12601 gcggcgccgc ccgggagcca gaacgtgctc tcgctcgcga cgtgggcgcg cgcgcatctc
    12661 gagcaccagc tcgacggcac gctcgccgat ttgctgctgc gagagctcgc cggcatgcgc
    12721 atcgcgctga agctcgagct cgcgccggat cccgcgaatt gcgacgaagc ggatcggcgc
    12781 atgctcgccg cgctcgctca gccgcgtcgc ctcgatcaaa tctggacgat cgcgcgcacg
    12841 ccgcgcttcc gcctgctctc gttcatccac tttctacgcg cggtcgacgc gctcgacgtc
    12901 gtgggtgtcg tcgccgacaa gagccagccg caccgctcga tcgatccgcg tcgcgaatcg
    12961 gctcgccgcc tgctgggcct acccgccgaa gcggatctcg agtcgatcaa gcgtgcgtat
    13021 cgccggctcg cgcgcgagct gcatcccgat ctgcaacccg atctcgatct gcacgagcgg
    13081 cgtacgctcg agcgtcggtt cgccgaagtg accgccgcgt acgaagcgct gctctgacgc
    13141 gctcagggcg gcgagaagat ccagccgtct tcggcgaaca cgccggtcgg cgattcccag
    13201 ccatcgcgcg cccagaggaa cgcggtgtac gccgagatga cggcgtcgaa gcaatggtcg
    13261 ttgcgcagca cgtcctcgcg agccatgcgt gattgcggcg cgaacgagag atcgtcgaag
    13321 cgggcgagca cttcggcgcg cgtcacccat ggatcggcat cgcgcttgta gccgcgggca
    13381 acgcgcggac cgcagagcgc ggcgatcgtc gccgcgggcg acacctcgag cagattgccg
    13441 tggagctgat agccggcgcc gcgcaggcgt cgacgcagct gaccggcacg cgcggtgatc
    13501 atgctgttcg cggtgcctag cgtcgacagc gggagaaggc cgcgctgata cgtcatgacg
    13561 acgtcggtcg cgcgatgcgc gtacggctga agccgtgctt gcggaggcgg cgacacgttg
    13621 cgcgatgaac cgcccacggt ttcggcggcg accttctgca tgagctggcg tccggtcgtg
    13681 cggagccaga cgactgccgg gtccgcgcat gcttcggtgc ctgggcacac gggcacggcg
    13741 cagcggccgc acgcggtctg cgtgagtgga gcgtcgaccg cgatcacgcg gttgggatcg
    13801 ggaatcgcga gccggccgat cagcgtgtcg tcggtccacg ggagcttgcc cgcacgagtc
    13861 gcgacctcga tgacctcggc ggtgccgcca gcgcccatcc ggagctcggc aacagcggtc
    13921 gtcttccctc ggcccccgcc gagatcgacg ccgacgaacc tggaaaatgc gcgctgcacc
    13981 ctctgtggtt ccctttgtag cacgttcgca tggccagaaa atgtctttaa gctctggcat
    14041 ttggccgaaa gtcttgagat acggtgcgtg ccctgtaagg gcagtacccc tgccgtcgtt
    14101 agtagtcgcg cctctcttct cttatggatc gcatcgccac gtttcgtagc ttcatctcga
    14161 agtccccgac ggatccgttc cctcggtacg gcctggcgat ggagcacaaa gggcgtggcg
    14221 agctcgacga ggcctggcgc gtgttcgagg agctgctcgc gcaatttccc gactacgtcg
    14281 cgacctacct catggcaggc ggaacactcg tcgcactcgg caagaaggcc gaagcggcgg
    14341 aggtctatcg caagggcatc gaagttgccc agcgccgcgg cgatcagcat gctcgcggcg
    14401 agctggaatc ggccctcggc gagatctctc cgacctaagc atcaacgctc aacaccgttc
    14461 gtcaacaaca ggagatctct cgatgaagct cgtaattacg ttcctgatgg ctgcggcgct
    14521 catggcatgc ggcaagtcga agaagccgga caccacgccc accaacacga cgggcacgac
    14581 cgagaagtcg acgacggacg gcgcgaccgg cgggcagacg tacggcaacg ctggcggtca
    14641 gacgactccg gccggcggcg gcaccgaccc ctgcgcgggc atgtaaccaa ccactttgct
    14701 tttgctgcgt ggcaggcgcc tagcagcgga ataaaaacca acagaggact agagagtatg
    14761 aagaagatcg cgattgttct gttcgttgtg gctgcggcgc ttgcaggctg tggcaagaag
    14821 aaggctccgg cggctccggc tggtggcgac accggctcgg cggctcccgc gggcgacacc
    14881 acgggctcgg cagcgggctc ggacgctggc tcggccgctc cggctgaggg tggcggcggc
    14941 atgtaagccg ctgcccggtg cgttgttttg caccggacac acggagacgc cggccctggt
    15001 ggtcggcgtt ttcgttttcg gctagaaata gcgccgaaat agccgggatg gagaaatggt
    15061 agactcgggg gtctcaaaag ccccttcccc ttaaaggagt gtcggttcga gtccgactcc
    15121 cggcacaaga gcgcttcggt cgcgaaaaat gcgagactgg gacgtgcgtc tggcggcatt
    15181 gattctgctc gcttcgctcg cctcctgccg tttgggcttt ggggaaagcg acgcgcctcc
    15241 aggcgacgac acttcctcgg cggccgacgc cacgttctcg atgccacctg gcgacgccgg
    15301 cagctgcgag ccggtcgcct gcaccaccgc cggcggtacg tgcgtcggca gcgtctgcca
    15361 gctcgtcgcc acgggcgaaa cgattctgtc ctgcccggcg ggcatgccgt gtcgtctggt
    15421 ctgcgacggg taccggttct gtcgtgaggg cgcacgctgc aacggcgcga cgtggtgcga
    15481 ggtcgtgtgc atcggattcc gtgcctgcca agccggcgtg cagtgcgatg cgagtgactg
    15541 cctcgtcacg tgcaatggcc ctgaagcgtg cgaggtcgga atcactgcga cgggcacgtg
    15601 cacggcacac tgctgcggcg attcgcaaac gtgcgcgggc ggcgtcggca cgtgcaccaa
    15661 ggataccgtc tgcgattgat ccgttgccga aaacgaaaac gccggaacaa agtccggcgc
    15721 gatcgcgatc agcagcgctg atcatccggt gcagaccccg gccgctttgc ggccgacgat
    15781 ccgcagcgga ttacatcccg ccggcgccac cctcagccgg agccgccgag ccgtcgccgg
    15841 ccggagccgc cgaacccgcg gtgtcgccac cagccggagt cgctgccggc ttcttcttgc
    15901 cgcagccagc aagcgccgcc gagaccacaa acagaacaat cgcaatcttc ttcatactct
    15961 ctagtcctct gttggtttgt ttccgccgct ggcgcctgcc aggcagcaaa atcgttattt
    16021 cgcctgggct ccgtaggtcg atccgccgta ggccgctccg ccatacgtcg tgccgcccgt
    16081 gccctgggtg gtgttctggg tctcgctgtt ggccggggtg gtcgaggact ttgcct
//
LOCUS       DATLBZ010000666        14099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8875935, whole genome shotgun sequence.
ACCESSION   DATLBZ010000666 DATLBZ010000000
VERSION     DATLBZ010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14099
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8875935"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(371..2623)
                     /locus_tag="VMZ53_32865"
     CDS             complement(371..2623)
                     /locus_tag="VMZ53_32865"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUS33356.1"
                     /translation="MRVIASLCACSLLVGCIGHHPEPQRPMHLQRVILYQNGIGYFER
                     GGHVQGETLKLEFGAHELDDVLKTLTVIDRLGAGVATVDVPTLKGDDKTIGLGVRMSA
                     GRVHDVKVSYAVPTPTWKAAYRVVMDDGASKKESLLQGWAMVNNASQEDWGDVQLTLA
                     TGAPMSFVHDLHTPQYVSRPDINGNLIAPTVIGPVSDEHARTGDTDRDGIVDIDDVCP
                     DSAEERDSFEDEDGCPDVDNDRDRILDRDDKCPNEPESYNGFEDEDGCPDRGRVVITD
                     TSLEVLDLVYFGKGSATIEQRSFAILDAIAATLKGNPTIRKVEVGGHASDDEADGWSL
                     ASRRAAAVRQYLIDRGVESRRLAIVPYGGTRPLDAKQTDAARAKNRRADFLITQREEF
                     ASERPSRRMPTKLDTKTMQASVHTASKPAEVAGTVRYVLSEPVTIKKGSSSMVSILNK
                     PIGAEDVYLFRPDGNAPGSDRHPFRAVRLINDSGFMLEPGPIAIFARGTFVGDSMVTR
                     LNVGETAWIPYAVDGSTTVTATTDASERPVKIVSISRGVLTVENAAIHVTTYSIDAGQ
                     EPAKRIFLRHAKLPGYTTKDLPPGTQDRGDSFLIPLPLQAGKSSSLTIEERQPRTQTM
                     HILDAGATQIGLYVDGSHLPDGIAEKLKAAIELRKEMGKIEESLEALRDRLADLTPRA
                     DDIRENLKAIEKVRGSDDLRRRLVASLTQVTTESDAIAKKLGLESESLANARTKLQDA
                     LRDISLDGDR"
     gene            complement(2668..3414)
                     /locus_tag="VMZ53_32870"
     CDS             complement(2668..3414)
                     /locus_tag="VMZ53_32870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUS33357.1"
                     /translation="MHHFRTVLFGALLVAGCENTPKPKDTTPSPKPADPAKPADPLPT
                     QPKPDPAPGADVAPPTAADLEAYTKDLPGTGSKLLMIMETSLGTFHCELYGDKAPMTV
                     ANWIGLATGKKPWLNPKTGNVERGKPYFDGLIFHRVIPGFMVQGGDPLGQGFGGPGYQ
                     FNDEIWQGQHVGAGVLAMANAGVRGGQGTNGSQFFIMEGDRPDLDSKHTVFGKCNEVD
                     LIKKIEAVPRDDNDKPNDPVSITKVTIQKG"
     gene            3582..4274
                     /locus_tag="VMZ53_32875"
     CDS             3582..4274
                     /locus_tag="VMZ53_32875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33358.1"
                     /translation="MSKLRIVLLSTLVAACSPNTSTKGQLITCTTDPDSGVIQSCQPG
                     GDDDDDDDTCQDIDEDGDGDPSDDDDDSATSDDDTIARTADSGPGGDSSESDDDEDDD
                     GLSDCVDDDDDNDGIDDDDDCDEQEGEDGDESDLPYDVRMAEGQSVTPISDAFAEKGA
                     QPQAVISVEMDGGGAGWRLAELAAGATFTVTAADCGHAGNRDVGRDRVIVTWRNASGA
                     VESDHLDLRYCE"
     gene            complement(4275..6341)
                     /locus_tag="VMZ53_32880"
     CDS             complement(4275..6341)
                     /locus_tag="VMZ53_32880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33359.1"
                     /translation="MKRLAFPLVAFVLACGNDHRPPDDDTDNNDVPFTECGADPASFV
                     RQTFLALDGRRPKSQAEVDVYVDLFEATKAAGSDPKEAVARAIMAQPEFGDRWLDATM
                     DAIHVQRLDVQTEASCWDSTNRATVDAGLAMAVRTMGATTQGDGKGTWTMLDLAKSAV
                     ALDDLTPIYRAQLFSMVAHPIPAANVPPIEAELARRADFGETFDAAYLHRDMVCLGCH
                     NSEHSVTDSDDEAADRHWPVPGLAELAVFGMSTGVAPDRAHAPFRVEGFVMDRSGSRP
                     WGWDPGCGVFSAIVGDDPAGIDGKLASISGKRSTVYTLEQALARGFDKLRGAGPPLEA
                     NGAITDPDAALAWLVTLKMTEDIWKKTTGTSLTIANYFPRNQAASDLLNSLATRYAQS
                     GYSLKALLVAIVQSDYFNRKPAELGCGKNPYTYPAVFDPWVTADADPVKRRNGPGDLV
                     TAIDARTLVAATNAALDWTAPPMASRFPDYGDGCETESCSQMQGYCNQFGSCCNAAKA
                     CMMNGVLPAEEVPFERGVGMFLRNSERGFRGLDFQARLVWEERYGACVRPRWVAADFI
                     DNIIAATAADPSATVGDVVAAIKDRLIGEPGVFDGSEASALAAIFGKTLDQPAAGVTA
                     EQARILCGSLLESPQFLLQGIAGRGGDRPKLTPAAASYDTVCTDVATHVAGVACTNGK
                     LTLAAH"
     gene            complement(6341..7819)
                     /locus_tag="VMZ53_32885"
     CDS             complement(6341..7819)
                     /locus_tag="VMZ53_32885"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HUS33360.1"
                     /translation="MGATSATALVAPYVMRKARAEFGVFPSGSEAAMLPASMRAKRVL
                     EVFLYGGLSPWETLYFVRNYGTPSDPQYANQQYYTFATSNDAMLGTTRCNAGSFSTQR
                     PFASDALGAQVEIGPFGNRLVSRTDVTDRMRLIVQKHNLEPHEAAVPQALTGRPVGQP
                     NAAGLGAHIQRARLASTPDRASPHSYVFATGGISSDNVAAAAAAGAHPGAARPLLIKT
                     DNASGFTNLLKRQAVGTAREHHDALVDVYATQYSNRLKWPSGERVRSARMDDFNVAFG
                     NQRKVDAISGVLSSDLFTTQGGNSCGTSKQKDIPLMGLSAAAKLLTHPTEPASYVCVS
                     DVGLYEASGGGGYDTHSDNARDTAVNFDHMLKNLLAIINAPGENDPRKLNLDDTLIIL
                     NTEFGRTPTAQGSTGRNHHPYGYVTAFIGGPTVKGIGGAIGPDAQATEYATPAQNRIA
                     ALLSLGIWPFAQEGFAVSDVPNASGELDAAVKSMQFCLGRTA"
     gene            8010..9680
                     /locus_tag="VMZ53_32890"
     CDS             8010..9680
                     /locus_tag="VMZ53_32890"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HUS33361.1"
                     /translation="MRVAVVLGVIGMSLIAREAHAQRWQDATAQCLGTTAEWTNKVEV
                     ADVDGDGHVDMLLANGGDYNSAGTAEPPRIWKNLANWTATTPHCEEITTSVVGSFTGL
                     SRMIKAADLDGDGDLEILTGGAWATQLKLFRRDAAGWTDVSAQLPQQPTNIGDAEFGD
                     VDGDGDIDIVLADWGTGNPQSNAGGRTKLYRNNGQATFTDETVADMPDMLVRWSWDLE
                     LADVDGDFDLDTLVSCKSCTSSLLFRNNGMGHFTDDPAALPHFANNYEFEPMDIDGDK
                     DLDLVTINDGPSVTEHVFVNNGMGMFADETSTRLAGTANPAGADDNTMVWLDGDNDGD
                     ADLVIGTLGRDRYLANNNGSFTLVETAPNSGANATSATLGLGIADFDEDGRLDLLQGQ
                     GEIQNSLADKVQLAGAMISFDTQPPVVVVGSPVEHDVRARIHDRVAPAHPHDFKKVVA
                     KVNGREIPMSWYGPMLWRGRLSLEDGTPSTYQVCATDRAGNEGCATYGGSSDDAGVGG
                     DAGTEPPGDKGGCCSSTRDARGSILLALLVLTRLGMRRRRAASRESSR"
     gene            complement(9625..10041)
                     /locus_tag="VMZ53_32895"
     CDS             complement(9625..10041)
                     /locus_tag="VMZ53_32895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33362.1"
                     /translation="MPKQERTTLLGVLSAIMLLLVLGLLYWRHALIVSWWVIAIAIAI
                     WLAFSVALRTHEKTWGKERPVLDWWSVPHFLAGVLFGLFGIGFAWAVSVATAWEGVEI
                     ASAVKEYPTNRAADIALAVTGWILAMLLGGGAFPIS"
     gene            complement(10042..11190)
                     /locus_tag="VMZ53_32900"
     CDS             complement(10042..11190)
                     /locus_tag="VMZ53_32900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33363.1"
                     /translation="MLLLLVASPAAADPETSPITDSNYSIELYDGIPIGNIALIGMGG
                     ATTALAIGTAGTLYNVSAPAVRPTTDTDHWSWDYHLDYVTGSFSSDYDNNGFVTDTTA
                     NQALTAGLGGRYGNWATAITFTGQDAPVAADFNGMPLEASTYRTQFAIAKWWPGIDTA
                     TGVSIDTAQFDFHPVPNDESLMNIAGAGLEIGAQWIPSGQDLRIGANLRSPIVGGDVS
                     GCDPSDCVGWTLPQHIVTATRFSAGAAYRFGPTPWNQLVKEFFRDEKAVTLAADVVVT
                     GSTENAFGLEAFGDHMLQRSGRHPSLSVRGGAEWEWKPGRLRIRGGSYWEPGRFVGVP
                     GRLHVTFGLEVRVFEFWFWGRKRRGRISLTADAAKYFSNGGLSVGFWH"
     gene            11253..14027
                     /locus_tag="VMZ53_32905"
     CDS             11253..14027
                     /locus_tag="VMZ53_32905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33364.1"
                     /translation="MPNTGETERPSASESAAALTSFASVPFRRRFVAPGLALGVLEVI
                     AIAYPLWSAFDLGALGADTLLRTALPVGIAAAFVWVLANAMWLWPLWLAVTARRAGEK
                     VNKDLAARAYRITHKGPVRILLLRTAVWTGGSALTGLFLWIYDDWSASRVAELTAIAA
                     IHSYVVSCVRAVWWAQIFGEIRARLFAAGSPLKKFDDSHFRRFLLVTMIVAGGVLAAQ
                     AAFSWYFVPIKKPEQYLQFQTYYPVATAVGLFAWTLFARWMTGDLRRYLAVSRGETRD
                     APPAAQIFRRAQALPYRLALLTITVWSVIAFVGAVIARLHLGFDFDDTLILATSTLVL
                     AVAGSIYEQLWHRDVLRPLLAHLTQRYRVPVRSIPPSLSLRSKLMLSFGGVVLLACGM
                     ALLWGFVQYKNLSTQAVARQSDIGLRWLRSELQADLGSAETKPTEEDVRTAISKIQKI
                     QKKEPEAANESVPNVIYYVAPSRQLTIVGGGPMGAPKLPWYTLSMIENESDTLLALHG
                     AQLTGKSGRLTVRWRGVPADLGAVAVFYPSYRGRGSSMVQPLKELLVFFFILFGACAG
                     IVAFTVAQFMAPIRRLEQRADAMARGELADPVAAGGEGDEIGRLTLALEEMRRALRDK
                     LRSTEEVNLDLERAVQMRTADLARKNRELAEALDKLGRAQEQIVRSEKLASIGQLVAG
                     IAHEINNPVNAIVNTVGPLEEAVQSIGTDPEAAKDVVEMIKVVQRGAQRTKAIVSALH
                     NYSRTDDESVVEFDIDRSIDDSLELLRHLLKQNVTVVRKYGEVGRVRGHAGQINQVFM
                     NLLTNAAQALAGRDSATITIETRGEPEGVEVKILDNGPGIPPEVLPRIWDPFFTTKDV
                     GEGTGLGLSIVHELVERHGGTIECKTKVGQGTTFTVKLPRKIQMSDRAKRPTGGGSEP
                     KRETAVTNPG"
BASE COUNT         2360 a         4657 c         4648 g         2434 t
ORIGIN      
        1 ggggcacacg tcctgcccgt gcgaacaccc aggcacacgt cctgcccgtg cgaacacccg
       61 ggcacgcgtc ctgcccgtgc gaacccgaaa ccctttctcc gcgtcctctg tgcctctgcg
      121 tcctctgcgt taaatccggg cacacgtcct gcgcgtgcga acacatgcgc ggcccacgtc
      181 ctgcccgtgc gaacccgaaa cccttttctc cgcgtcctct gtgcctctgc gtcctctgcg
      241 ttaaatccgg gcacacgtcc tgcccgtgcg gaggtgtgcg aacacccggg cacacgtcct
      301 gcccgtgcga acgggcacgc gtcctgcccg tgcgaacgac ccgcggcaca ggcgcggcac
      361 ggacgcgcga ctagcgatcg ccgtcgaggc tgatatcgcg cagcgcatcc tgcagcttcg
      421 tgcgcgcgtt tgcgagcgac tcactttcga gtccgagctt tttggcgatc gcgtctgact
      481 ccgtcgttac ctgcgtgagg ctcgcgacga ggcggcggcg caggtcgtcc gagccgcgga
      541 ccttctcgat cgctttgagg ttctcgcgga tgtcatcggc gcgcggcgtg aggtcggcga
      601 ggcggtcgcg cagcgcctcg agcgactcct cgatcttgcc catctctttg cggagctcga
      661 tggcggcctt gagcttctcg gcgatgccgt cggggaggtg gctgccatcg acgtagaggc
      721 ctatctgcgt cgcgcccgcg tcgaggatgt gcatcgtttg cgtgcggggc tggcgctctt
      781 cgatcgtgag cgagctcgac ttgccggctt gcagcggcag cgggatcagg aacgagtcgc
      841 cacgatcttg ggtgccgggt ggcagatcct tcgtcgtgta gcctggcagc ttggcgtggc
      901 gcaggaagat gcgctttgcg ggttcttggc cggcatcgat cgagtatgtg gtgacgtgaa
      961 tcgccgcgtt ctcgacggtg agcacgccgc gcgagatcga gacgatcttg acggggcgct
     1021 cgctcgcgtc ggtcgtcgcg gtgaccgtcg tcgagccatc gacggcgtaa gggatccagg
     1081 ccgtctcgcc gacgttgaga cgcgtcacca tgctgtcacc gacgaacgtg ccgcgcgcga
     1141 agatcgcgat cggacccggc tccagcatga agccggagtc gttgatcagg cggacggcgc
     1201 ggaagggatg tcgatcggag cccggtgcgt tgccgtcggg gcggaatagg tagacgtcct
     1261 cggcgccgat tggcttgttg aggatcgaca ccatcgacga gctgcctttc ttgatagtga
     1321 ccggctcgga cagcacgtag cgcacggtgc ccgcgacctc ggcgggcttg ctcgccgtgt
     1381 gcacgctcgc ttgcatcgtc ttcgtgtcga gcttcgtcgg catgcgacgc gacgggcgct
     1441 ccgacgcgaa ttcttcgcgc tgcgtgatga ggaagtcggc acggcggttc ttcgcgcgcg
     1501 cggcgtccgt ctgcttcgca tcgagcgggc gcgtcccgcc gtacggcacg atcgcaagcc
     1561 ggcgcgactc gacgccgcgg tcgatcaagt actggcgtac cgccgccgca cgacgcgaag
     1621 cgaggctcca gccatcggcc tcgtcgtcgc tcgcgtggcc gccgacctcg actttgcgga
     1681 tcgtcgggtt gcctttcagc gtcgcggcga tcgcatcgag gatcgcgaag gagcgctgct
     1741 cgatcgtcgc cgagcccttg ccgaagtaga cgaggtcgag cacttcgagc gacgtatcgg
     1801 tgatgacgac gcgtccccgg tcgggacagc cgtcctcgtc ctcgaagccg ttgtacgact
     1861 cgggctcgtt cgggcatttg tcgtcgcgat cgaggatgcg gtcgcgatcg ttgtcgacgt
     1921 cggggcagcc atcttcatcc tcgaagctgt cgcgctcctc ggcgctgtcg ggacagacgt
     1981 cgtcgatatc gacgatgccg tcgcggtcgg tatcacccgt gcgcgcgtgc tcgtcgctga
     2041 ccgggccgat gaccgtcggc gcgatcagat tgccgttgat atcgggacgg ctcacatact
     2101 gcggcgtgtg gagatcgtgg acgaacgaca tcggcgcgcc cgtcgcgagc gtgagctgca
     2161 cgtcgcccca gtcctcttgc gaggcgttgt tcaccatcgc ccagccctgc aggaggctct
     2221 ccttcttact tgctccgtcg tccatgacga cgcgatacgc ggctttccac gtcggcgtcg
     2281 gcacggcgta gctgaccttc acgtcgtgca cgcgtcccgc gctcatgcga acgccgaggc
     2341 cgatcgtctt gtcgtcgccc ttgagcgtcg gcacgtcgac ggtcgcgaca cccgcgccga
     2401 ggcgatcgat caccgtcagc gtcttgagga catcatcgag ctcgtgcgcg ccgaattcga
     2461 gcttcagcgt ctcgccctgc acgtggccgc cgcgctcgaa gtagccgatg ccgttctgat
     2521 agaggatgac ccgctgcagg tgcatgggcc gctgcggctc gggatgatgg ccgatgcagc
     2581 ccacgagcag cgagcacgca cagagcgaag cgatcacgcg catgccggca cagtgctctg
     2641 cgcccggccg gcggttacac gcgcggctta tcccttctga atcgtgactt tggtgatcga
     2701 caccgggtcg ttcggcttgt cgttatcgtc acgcggcacg gcctcgatct tcttgatcag
     2761 gtcgacctcg ttgcacttgc cgaagaccgt gtgcttcgag tcgaggtcgg gacggtcgcc
     2821 ttccatgatg aagaactggc tgccgttcgt gccctgaccg ccgcgcacac ctgcgttcgc
     2881 catcgcgagg acgcctgcgc cgacgtgctg gccttgccag atctcgtcgt tgaactgata
     2941 gcccggacca ccgaagccct ggccgagcgg atcgccgccc tggaccatga accccggaat
     3001 cacgcggtgg aagatcaggc catcgaagta cggcttgccg cgctcgacgt tgcccgtctt
     3061 cggattgagc cacggctttt tgcccgtcgc gaggccgatc cagttcgcga ccgtcatcgg
     3121 cgccttgtcg ccgtagagct cgcagtggaa cgtgccaagg ctcgtctcca tgatcatgag
     3181 gagcttcgag cccgtgccgg ggaggtcctt cgtgtaggcc tcgagatcag ccgcggtcgg
     3241 cggcgcgacg tccgctccag gcgccggatc cggcttcggc tgcgtcggca gcggatcggc
     3301 gggctttgcg ggatcggcgg gcttgggcga cggcgtcgta tccttcggct tgggggtgtt
     3361 ctcacaaccg gccacgagga gggcgccgaa caggactgta cgaaagtggt gcatggcgcc
     3421 gggaacatgg cgcaaagccg acagctgtgc aactcgcggc aggtgtacga gcgtgttgtg
     3481 caacgtgctt cgcaccccca tgtgcaagtc gctggaacac gtgtcacgcg cggtggcacg
     3541 tcggtcgcag agagtcgagg catcaaccgg gaggtacttt gatgtcaaag cttcgcatcg
     3601 ttctgctctc gaccctcgtc gccgcgtgct cgccgaacac ctcgacgaaa ggtcaactga
     3661 tcacgtgcac gaccgatcca gacagcggag tcattcagag ctgccagccc ggcggcgatg
     3721 acgacgacga cgacgacacg tgccaagaca tcgacgagga cggcgacggc gacccgtccg
     3781 acgacgatga cgatagcgcg acgagtgacg acgacacgat cgctcgcacc gccgacagcg
     3841 gccccggtgg cgactcgtcc gagtcggacg atgacgaaga cgacgacgga ctctcggact
     3901 gcgtcgacga cgacgatgac aacgacggca tcgacgacga cgacgattgc gacgagcaag
     3961 agggcgagga cggcgacgag tccgacttgc cgtacgacgt ccgcatggcc gagggccaga
     4021 gcgtcacccc gatctccgac gcctttgccg agaagggcgc gcagccgcaa gccgtcatct
     4081 ccgtcgaaat ggacggtggt ggagcaggct ggcgcctcgc cgagctcgcg gcaggcgcga
     4141 cgttcacggt cacggccgcc gactgtggcc acgcgggcaa ccgcgatgtc ggccgcgatc
     4201 gcgtgatcgt cacgtggcgc aacgcgagtg gcgccgtcga gagcgaccac ctcgatctgc
     4261 gctactgcga gtaactagtg cgcggcgagc gtgagcttgc cgttcgtgca cgcaacgccc
     4321 gcgacgtggg tcgcgacatc ggtgcagacc gtgtcgtagc tcgcggccgc gggcgtcagc
     4381 tttggacgat caccaccacg gcccgcaata ccctgcagta gaaactgcgg tgactcgagc
     4441 agcgagccgc acaggatgcg cgcttgctcg gcagtcacgc cggcggcggg ctgatcgagc
     4501 gtcttgccga agatcgccgc aagcgcggaa gcctcgctgc cgtcgaacac cccgggctcg
     4561 ccgatgagcc gatccttgat cgccgcgacg acatcaccga cggtcgccga tggatcggca
     4621 gcagtcgcag cgatgatgtt gtcgatgaag tccgcggcga cccaccgcgg gcgcacgcac
     4681 gcgccatagc gctcttccca gacgaggcgc gcttggaagt cgaggccgcg gaacccgcgc
     4741 tcgctgttgc gcaggaacat gccgacgccg cgctcgaacg gaacttcctc ggcggggagg
     4801 acaccgttca tcatgcaggc cttcgcggcg ttgcagcacg agccgaactg gttgcagtag
     4861 ccctgcatct ggctgcagct ctcggtctcg cagccgtcgc cgtagtccgg gaagcgcgat
     4921 gccatcggcg gcgccgtcca atcgagcgcg gcgttcgtcg cagcgacgag cgtgcgtgca
     4981 tcgatcgcgg tgacgagatc gccgggaccg ttgcgccgct tgaccgggtc cgcgtccgca
     5041 gtgacccacg gatcgaacac ggccggatac gtgtacgggt tcttgccgca gccgagctcg
     5101 gccggcttgc gattgaagta gtccgactga acgatcgcga cgagcaatgc cttgagcgag
     5161 tagccgctct gcgcgtagcg cgtcgcgagg ctgttcaaga gatcacttgc cgcttgattg
     5221 cgcgggaagt agttcgcgat cgtgagcgag gtgccggttg tcttcttcca gatgtcctcg
     5281 gtcatcttca gcgtgacaag ccacgcgagc gccgcgtcgg gatccgtaat cgcgccgttc
     5341 gcttcgagcg gaggaccggc gccgcggagc ttgtcaaagc cgcgcgcgag tgcttgctcg
     5401 agcgtgtaga ccgtcgagcg cttgccgctg atgctggcga gcttgccgtc gatgccagct
     5461 ggatcgtcgc cgacgatcgc cgagaacaca ccgcagccag gatcccagcc ccacggccgc
     5521 gagccgctgc gatccatgac gaaaccttcg acgcggaacg gcgcatgcgc gcgatccggc
     5581 gcgacgcccg tcgacatgcc gaacaccgcg agctcggcga ggcctggaac gggccagtgc
     5641 cgatcggctg cctcgtcatc gctgtcggtg accgagtgct cgctgttgtg acagccgagg
     5701 cacaccatgt cgcgatgcag gtacgccgcg tcgaacgtct cgccgaagtc ggcgcggcgc
     5761 gcgagctctg cttcgatcgg cgggacgttc gccgctggaa tcggatgcgc gaccatcgag
     5821 aacagctgcg cgcgatagat cggcgtcagg tcgtcgagcg ccacggccga cttcgcgagg
     5881 tcgagcatcg tccacgtgcc cttgccatcg ccttgcgtcg tcgcgcccat cgtgcggacc
     5941 gccatcgcga ggcccgcgtc gaccgtcgcg cgattcgtcg agtcccagca cgatgcttcg
     6001 gtctgcacgt cgagccgctg gacatggatc gcatccatcg tcgcgtcgag ccaacgatcg
     6061 ccgaactccg gctgcgccat gatcgcgcgt gcgacggctt cttttggatc gctgccggcg
     6121 gccttcgtcg cctcgaagag atcgacgtag acatcgacct cggcttggct cttcggccgg
     6181 cgcccgtcga gcgcgaggaa tgtctgccga acgaacgagg ccggatcggc gccgcactcg
     6241 gtgaacggga cgtcgttgtt atccgtatcg tcgtcgggcg gacgatggtc gttgccgcac
     6301 gcgagcacga acgcgacgag cgggaaggcg aggcgcttca tcacgctgtc ctcccaaggc
     6361 agaactgcat ggacttcacc gcagcatcga gctctcccga tgcattcggc acgtccgaca
     6421 ccgcgaagcc ttcttgcgcg aacggccaga tgccgagcga gagcagagcc gcgatgcgat
     6481 tctgcgcggg cgtcgcgtac tccgttgctt gcgcatcggg accaattgca ccgccgatgc
     6541 ccttcaccgt ggggccaccg atgaacgcgg tgacatagcc gtacgggtga tggttgcgcc
     6601 ccgtcgagcc ttgcgccgtc ggtgtgcgac caaattccgt attgaggatg atcagcgtgt
     6661 cgtcgaggtt gagcttgcgc ggatcgttct ctccaggcgc gttgatgatc gcgaggaggt
     6721 tcttcagcat gtggtcgaag ttgaccgcgg tatcgcgcgc gttgtccgag tgcgtgtcgt
     6781 aaccgccacc gccggacgct tcgtagagac cgacatcact cacgcacacg tacgatgccg
     6841 gctcggtcgg atgcgtcagc agcttcgcgg cagccgacag tcccatcagc gggatgtcct
     6901 tctgcttcga cgtgccgcag gagttgccgc cctgcgtggt gaacagatcc gacgagagca
     6961 cgccgctgat cgcatcgacc ttgcgctggt tgccgaacgc gacattgaag tcgtccatgc
     7021 gcgcactgcg cacgcgttcg ccgctcggcc acttgagccg gttggaatat tgggtcgcat
     7081 acacgtcgac gagcgcatcg tgatgctcgc gagcggtgcc gacggcctgg cgcttcagca
     7141 gattcgtgaa gccactcgcg ttatctgtct tgatgagcag tggccgggcg gcgccgggat
     7201 gcgcaccagc tgctgccgct gcagcaacgt tgtcgctcga gatgccgccc gtcgcgaaca
     7261 cgtacgaatg tggcgatgcg cgatcaggcg tcgaggcaag acgtgcgcgc tggatgtgag
     7321 caccgagacc ggctgcgttg ggctggccaa ccggccggcc ggtgagcgct tgcggaacgg
     7381 cagcttcgtg cggctcgagg ttgtgcttct gcacgatgag gcgcatgcga tcggtgacgt
     7441 ccgtgcgcga gacgaggcga ttgccgaacg gaccgatctc gacttgcgcg ccgagcgcat
     7501 cgctcgcgaa cggccgctgc gtcgagaacg agccggcgtt gcagcgcgtc gtgccgagca
     7561 tcgcatcgtt gctcgttgcg aacgtgtagt actgctggtt cgcgtactgc ggatcgctcg
     7621 gcgtgccgta gttgcgaacg aagtagagcg tctcccacgg tgagagccca ccgtagagga
     7681 agacttcgag cacgcgcttc gcacgcatgc tcgcgggcag catcgccgct tcggatccgc
     7741 tcgggaacac gccgaattcg gcgcgtgcct tgcgcatcac gtagggcgcg acgagagctg
     7801 tcgcgctggt tgcccccacg aggcctagga aacgtcggcg tcgcatggcc ggtacggtgc
     7861 acccgttggc tcccgaccgt caccacaaaa ggctattgcc gccttgcagt ctgatgcccc
     7921 gggggcgcag cagcgaatgc gcaattggtg aaggcagttg tcagatttct gaggtgcggc
     7981 cgaaggccaa accgtgttct cgtcgcgaca tgcgggttgc ggtcgtgctt ggagtcatcg
     8041 ggatgtcact tatcgcgcgt gaggcgcatg cgcagcgttg gcaagacgcg accgcgcaat
     8101 gcctcggcac gacggcggaa tggacgaaca aggtcgaggt cgccgacgtc gatggcgatg
     8161 gccacgtcga catgctgttg gcgaacggcg gcgactacaa ctcggccgga accgcggagc
     8221 cgccgcgcat ctggaagaac ctcgcaaact ggacggcgac aacgccgcac tgcgaagaga
     8281 tcacgacgag cgtcgtggga tcgttcaccg ggctctcgcg gatgatcaaa gccgccgatc
     8341 tcgacggcga cggcgacctc gagatcctga cgggcggcgc gtgggccacg caactgaagc
     8401 tcttccgtcg cgacgccgcg ggctggacgg acgtcagcgc acagctgccg caacagccga
     8461 cgaacatcgg tgacgccgaa ttcggtgacg tcgacggcga tggcgacatc gatatcgtgc
     8521 tcgcggactg gggcacggga aatccgcagt cgaacgccgg tggccgcacg aagctctatc
     8581 gcaacaacgg ccaggccacg ttcaccgacg agaccgttgc cgacatgccc gacatgctcg
     8641 tgcgctggtc atgggatctc gagctcgcgg atgtcgatgg cgacttcgat ctcgacacgc
     8701 tcgtctcgtg caagagctgc acgtcgagcc tgctgtttcg caacaacggc atgggccact
     8761 tcacggacga ccccgccgcg ctgccgcact tcgcgaacaa ctacgagttc gagccgatgg
     8821 acatcgatgg tgacaaagac ctcgatctcg tcacgatcaa cgacggcccg agcgtcaccg
     8881 agcacgtgtt cgtcaacaac ggcatgggca tgttcgccga cgagacatcg acgcggctcg
     8941 ctggcaccgc gaatcccgca ggcgccgatg acaacacgat ggtgtggctc gacggtgaca
     9001 acgacggcga cgccgatctc gtgatcggaa cgctcggccg cgatcgctac ctcgcaaaca
     9061 acaacggctc gttcacgctc gtcgagacgg caccgaacag cggcgcaaac gcgacctctg
     9121 cgacgctcgg cctcggcatc gctgacttcg acgaggatgg ccgcctcgat ctcctgcaag
     9181 ggcagggcga gatccagaat tcactcgccg acaaggtgca gctcgccggc gcgatgatct
     9241 cgttcgatac gcagccgcct gtcgtcgtcg tcggctcgcc cgtggagcac gacgtgcgtg
     9301 cgcggattca cgaccgcgtt gcgcccgcgc acccgcacga cttcaagaag gtggtcgcga
     9361 aggtcaacgg ccgtgaaatt ccgatgagct ggtacgggcc gatgctgtgg cgtggccggc
     9421 tgagcctcga agatgggacg ccgtcgacgt accaagtctg cgcgaccgat cgcgccggca
     9481 acgaaggctg cgcgacctac ggcggaagta gcgacgacgc cggcgtcggt ggcgatgcgg
     9541 gaacggagcc tcccggcgac aagggaggct gctgcagctc gacgcgcgat gcccgaggtt
     9601 cgatcctgct cgcgctgctc gtgctcacga gattgggaat gcgccgccgc cgagcagcat
     9661 cgcgagaatc cagccggtaa ccgcgagcgc gatatcagcc gcgcgattcg tcggatactc
     9721 cttcaccgcc gacgcaatct cgacgccttc ccacgcggtc gcgacgctga cggcccacgc
     9781 gaagccgatg ccgaacaggc cgaacagcac gccggcgagg aagtgcggaa cgctccacca
     9841 gtcgaggacg ggccgctctt tgccccacgt cttctcgtgc gtgcgcagcg cgaccgagaa
     9901 cgcgagccag atcgcaatcg cgatcgcgat cacccaccac gacacgatca gtgcgtggcg
     9961 ccagtacaaa agcccgagca cgagcagcaa catgatcgcg ctcaggactc cgagcagcgt
    10021 cgtgcgctct tgtttcggca tctaatgcca aaagcctacc gagagtcctc cattcgagaa
    10081 gtacttggct gcgtctgccg tcagtgagat ccggccgcgg cgctttcgcc cccagaacca
    10141 gaactcgaac acgcgaacct cgagtccgaa tgtcacatgg aggcgccccg gcacaccgac
    10201 gaagcgtccg ggctcccagt aacttccgcc gcgaatccgc agacgtcccg gcttccactc
    10261 ccactccgcg cctccgcgca cgctgagcga tggatgacgg ccggagcgct gcagcatgtg
    10321 gtcgccaaac gcttcgaggc cgaacgcatt ctccgtcgag ccggtgacga ccacatcggc
    10381 cgcaagcgtg acggccttct cgtcgcggaa gaactctttc acgagctggt tccacggcgt
    10441 cgggccgaag cgatacgcgg cgcccgccga gaaccgcgtc gcggtgacga tgtgctgcgg
    10501 cagcgtccag ccgacgcaat cgctcgggtc gcaacccgag acgtcgccgc cgacgatcgg
    10561 gctccttaga tttgcgccga tgcgcagatc ctggccgctc gggatccact gcgcgccgat
    10621 ctcgaggccg gcgccggcga tgttcatcag tgactcgtcg ttcgggaccg gatgaaagtc
    10681 gaactgcgcg gtgtcgatcg agacgccggt cgcagtgtcg atgcccggcc accacttcgc
    10741 gatcgcgaac tgcgtgcggt acgtcgatgc ttcgagcggc atgccattga agtccgcggc
    10801 gaccggcgcg tcttgcccgg tgaacgtgat cgccgtggcc cagttgccgt agcgtccacc
    10861 gaggcccgcg gtgagcgctt gattcgccgt cgtgtcggtg acgaacccgt tgttgtcgta
    10921 gtccgacgag aacgagccgg tgacgtagtc gaggtggtag tcccagctcc agtgatcggt
    10981 atccgtcgtc ggacggaccg caggcgccga gacgttgtag agcgtgcccg ctgtaccgat
    11041 cgcgagtgcg gtcgtcgcgc cgcccatgcc gatgagcgcg atgttgccga tcgggatgcc
    11101 atcgtagagc tcgatggagt agttgctgtc ggtgatcggc gacgtctcgg gatccgcagc
    11161 cgcgggggac gcgactagca gtaacaacac cccaattctg ccggctcgcg ccacgcctcc
    11221 atcatacgct gaagtgcact atcctcagcg ggatgccgaa caccggcgag acggaacggc
    11281 cgtctgcgag cgaaagcgct gcggcgctga cgtcgttcgc cagcgttccg tttcggcgtc
    11341 ggttcgtcgc gccaggcctc gcgcttggcg tgctcgaggt gatcgcgatc gcctaccccc
    11401 tgtggtccgc gttcgatctc ggcgctctcg gtgccgatac actgctgcgg accgcgctgc
    11461 cggtcggcat cgccgcggcg tttgtgtggg tgctcgcgaa tgcgatgtgg ctgtggccgc
    11521 tgtggcttgc cgtcactgcg cggcgcgccg gcgagaaggt caacaaggac ctcgccgcac
    11581 gcgcgtatcg catcacgcat aaaggacccg ttcgcatcct tctgctccgc acggcggtgt
    11641 ggactggcgg ctctgcgctc accggcttgt tcctgtggat ctacgacgac tggtctgcgt
    11701 cgcgcgttgc cgagctcaca gcgatcgcgg caatccactc gtacgtcgtg tcgtgcgtgc
    11761 gcgcagtctg gtgggcgcag atcttcggcg agattcgcgc tcggctcttc gcggcggggt
    11821 cgccgctgaa gaagttcgac gacagtcact ttcggcgctt tctcctcgtc acgatgatcg
    11881 tcgccggagg cgtgcttgcc gcgcaggcgg cgttctcctg gtacttcgtc ccgatcaaga
    11941 agccggagca gtacctccaa ttccagacgt attacccggt cgcgactgcc gtcggcctgt
    12001 tcgcgtggac actgttcgcg cgctggatga ccggcgacct ccgccgttac ctcgcggtct
    12061 ctcgtggcga gacgcgcgac gcgccaccgg ccgcacagat cttccggcgc gctcaggcgc
    12121 ttccgtatcg gctcgcgctc ctgacgatca cggtgtggtc ggtgatcgcg tttgtcggtg
    12181 cggtgatcgc tcgccttcac ctcggcttcg atttcgacga caccctcatc ctcgctacgt
    12241 ccacgctcgt cctcgccgtc gccggatcga tctacgagca gctgtggcat cgcgatgtgc
    12301 tccgtccgct gctcgcgcac ctcacccagc gctaccgagt gccggtgcgt tcgatcccac
    12361 cgtcgctgtc gctgcgtagc aagctcatgc tctcgttcgg cggcgtcgtg ctgctcgcgt
    12421 gcggcatggc gctgctctgg ggcttcgttc agtacaagaa cctgtcgacg caggccgtcg
    12481 cgcgccagtc ggatatcggt ctgcgctggc tgcgctcgga gctgcaagcc gacctcggca
    12541 gtgcggagac gaagcccacg gaagaggacg ttcgcacggc gatcagcaag atccagaaga
    12601 tccagaagaa ggaacccgag gcggcgaacg agtctgtacc gaatgtcatc tattacgttg
    12661 ccccgtcgcg ccagctcacc atcgtcggcg gtggaccgat gggggcaccg aagttgcctt
    12721 ggtacacgct ctcgatgatc gagaacgagt cggacacgct cctcgcgcta cacggcgcgc
    12781 agctcacggg caagtccggt cgtttgaccg tgcggtggcg cggcgtgccc gcggacctcg
    12841 gcgccgtcgc tgtcttctat ccgtcgtatc gaggtcgcgg ctcgtcgatg gtgcagccgc
    12901 tcaaagagct gctcgtcttc ttcttcatcc tgttcggagc atgcgcaggc atcgtcgcgt
    12961 tcacggtcgc gcaattcatg gcgccgatcc gccgtctcga acaacgcgcc gacgcgatgg
    13021 cccgcggtga gctcgcagat ccagtcgccg caggcggcga gggcgacgaa atcggtcgcc
    13081 tcacgctcgc gctcgaagaa atgcgccgcg cccttcgcga caagctgcgc tcgacggaag
    13141 aagtcaacct cgacctcgag cgcgccgtgc aaatgcgcac cgccgacctc gctcgcaaga
    13201 accgcgagct cgccgaagcg ctcgacaagc tcggtcgcgc gcaagagcag atcgtccgct
    13261 ccgagaagct ggcctcgatc ggtcagctcg tcgccggcat cgcgcacgag atcaacaacc
    13321 ccgtcaacgc gatcgtcaac accgtcggcc cgctcgaaga agccgtccaa tcgatcggca
    13381 cggatcccga ggcagccaag gacgtcgtcg agatgatcaa ggtcgtccag cgcggcgccc
    13441 agcgcaccaa agcaatcgtg tctgcactcc acaactattc gcgcaccgac gacgaatccg
    13501 ttgtcgagtt cgacatcgat cgctcgatcg acgactcgct cgagctgctg cgccacctgc
    13561 tcaagcagaa cgtcacggtc gtgcgcaagt acggcgaggt cggccgcgtc cgcggtcacg
    13621 caggccagat caaccaagtc ttcatgaacc tcctgacgaa cgccgcgcaa gcgctcgccg
    13681 gccgcgactc cgcaacgatc acgatcgaga cgcgcggtga gcccgaaggc gtcgaagtca
    13741 aaatcctcga caacggcccc ggcatcccac ccgaggtgct gcctcgcatc tgggatccgt
    13801 tcttcacgac gaaagacgtc ggcgaaggta ccggcctcgg cctctcgatc gtgcacgagc
    13861 tcgtcgagcg ccacggcggc accatcgagt gcaagacgaa ggtcggccaa ggcacgacgt
    13921 tcaccgtgaa gctgccgcgc aagatccaga tgagcgatcg cgcgaagcgg ccaacaggcg
    13981 gtggctccga gccgaagcgc gagacggccg tgaccaaccc gggctgagct acggctcgta
    14041 cgcgccgact gcaggcaggg cagggcgcgc gttgccgcga atgtcgacgg ccggcgatt
//
LOCUS       DATLBZ010000667         9447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8882610, whole genome shotgun sequence.
ACCESSION   DATLBZ010000667 DATLBZ010000000
VERSION     DATLBZ010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8882610"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VMZ53_32910"
     CDS             <1..811
                     /locus_tag="VMZ53_32910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HUS33365.1"
                     /translation="LVINGDHVKISATKDPAAIGWKDLGVDIVIESTGVFVDKAGAGK
                     HIQGGAKKVIISAPAKEPDVTLCMGINTEMYKPGEHHVISNASCTTNCLAPVAKVLHE
                     TFGIVSGIMTTIHSYTNDQQILDLPHKDLRRARAAALSMIPTTTGAAKALKEVLPAMK
                     GKLDGMAIRVPTPNVSLVDLTARLEKQATVEQVNDAFRKAASGPLKGILAVAEEPLVS
                     CDYNGDKHSSTVDAALTTAIGDQVKVLSWYDNEMGYSQRLFDLARFVGERL"
     gene            812..2011
                     /locus_tag="VMZ53_32915"
     CDS             812..2011
                     /locus_tag="VMZ53_32915"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HUS33366.1"
                     /translation="MALPGGIKSVEQLGVEGKRVFVRVDYNVPLDKKTKKITDDARIR
                     ATLPTVKHLIDRGARIVLGSHLGRPDGQVKPEFSLEPVAAYLADLLKTDVALADEPTG
                     DGARKVVSDLRDGQIAMLENLRFNPGEEANDETFARTLASYCDVYVNDAFGTAHRAHA
                     STAGMVKFVAEKGAGFVMAKEIEILSRLLGDVDRPYVAVLGGAKVSDKIEVLDALLER
                     VNAIVIGGAMANTFLEAQGKNVGKSKVEKDKLPIARNFLRKASEKKVAVHLPTDVLVG
                     SGLDDTNAVQARVDAIPGESMALDIGPGSIELFRRILTEARTVFWNGPMGVFEKPQWA
                     NGTVAVAKALADNKLALTVVGGGDSAAAIAQAGLADKVSHVSTGGGASLEFVQGLDLP
                     GIAALRP"
     gene            2174..2536
                     /locus_tag="VMZ53_32920"
     CDS             2174..2536
                     /locus_tag="VMZ53_32920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS33367.1"
                     /translation="MVDDSKVMRDMVAACLRPLGEVTFEFAASGLEAIERLALATFNL
                     VVLDLNMPDVGGVEVIEFIRSQDKLRSLPILVVTTRGDDASRTRVLEAGASGFLAKPF
                     APEQILTDVRGLLERAAP"
     gene            2533..4536
                     /locus_tag="VMZ53_32925"
     CDS             2533..4536
                     /locus_tag="VMZ53_32925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HUS33368.1"
                     /translation="MKDTVDLQEFVGGFIAESDQLVASAKAHLLEIERASEANQPSAK
                     LVRELFRAMHTLKGLAGMMGVQPIVDIAHALETVLRSADLAGGQLGSRACELGFIGVR
                     AIAERVRAVADHKPVPPAPESLIDELARVDVSGNTQSAPLPVVASWDKKLNASERQQV
                     VSALEANRKAYTLMFAPSDQHAARGVSISSVRASLGALGEIVKVAPRSVPATDTAPAG
                     LVFEILVVSDADVEALARAAATTPAQVAAVEIEVSSAPALESLPATEDELTPIGRSVV
                     RVELSRLDELQEQLSALVVTRFRLERQIASLSASGNDIRVLREIADSQGRQLRDLRRA
                     ILRVRMVRLSEVLEPLTLLVRSMVKPGIKEARLELDVRDSELDKAVADRLLPALVHLV
                     RNAVDHAIEPVVERERAGKSRAGTVRIESREGAGNQLVLSVVDDGRGIDRAVIARRAR
                     KELADDDAVLEVLTAPGFSTREKVTETSGRGIGMDIVRRIVASDLGGELSMTTKVGQG
                     TTFTLEVPLTIAIIDVFSFECAGQPFVVPVTTVEEIFELTEQRRIDSPAALRGRLPVA
                     LLERRGRPLPLVELSKMLRISTAESAASKALVVRRQNEPLAFAVDRMLGRHEVVIRPI
                     EDVLARVPGIAGATDLGDGRPTLLLDLVELAAGISGWRTELPS"
     gene            4533..4967
                     /locus_tag="VMZ53_32930"
     CDS             4533..4967
                     /locus_tag="VMZ53_32930"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HUS33369.1"
                     /translation="MTKLHVAFKVGTAEYIISSDHVVHLEAYAGATRVPGAPEFVAGL
                     VQVRGKLVPVVDLRTRFGLAPGEQTIDSRVIVVKVGTRTAGLLVDSARDVLRIDEEKF
                     EEPPEMVRDQANGFVRAVTTQANRLLFVVDVPRVIGEEIAHA"
     gene            4960..7476
                     /locus_tag="VMZ53_32935"
     CDS             4960..7476
                     /locus_tag="VMZ53_32935"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HUS33370.1"
                     /translation="MRNSFGADLRQVDAVASSLRETADKAEQIELAASRLAAGGNENA
                     AGTDQARVAIESILSNIEETAAATEELTRSQAGVASSARDLMQGLETTAAGMQELASS
                     VAAVRKDADALARTADSTAATLEESARSIKATSQNATELAAASQQLAATATETTSSVT
                     DMAARGAENASTIEEVATTVEQMSAGIARIAGDSKQVGDRVTSIATGLTAVSASLEAV
                     ARDAAESATSVEETAATSEELARSVRGVADLAKQLEDSAINSASTVAEVAASVEQVAA
                     TAEKNAAVVDSTATAIEQAARSAQTVAQAASQVGTLASASSSAASQLETATRKIVGAV
                     EGTRAHSDRVLASAQAGGATIGRTIQGLVKIREAMSESTVVMKDMGKRAEEIGDIVQA
                     INLIADRTNLLSLNASIEAARAGEHGRGFAVIAEEIRALADRAAAAAGDVAKIVRGLQ
                     GTAREAVSANAEGLRVADEGAQLSENARSSLQAILDGVSTVDRSIREISISANEQAQA
                     VQTVVVTTTRLSEEGRAISRAGEEQMQATQSLARASAEMRTMAKQTRDATTEQARALR
                     DLVKTNQLLSESAQKVSRATTEQATSASQLARATTQLRAATNRTTNAMAEQQRSVLAM
                     AANAQDSATATARTVTAITEQARGSAEIARAMDGARKQAVQTARAVGEQARASKDIEA
                     TSREVARLAVELTSATNEQSQALQLLVKDGEEVRRVARQTARAVGEQADALSALANAG
                     QRQTSLVQSVARAANEQTSSTEQVATAMRDVRGRTREIATTLTEQVKSSTTTAHDVAV
                     IAQEIAGLRRANVEQAAQVTALGASLGGNGAAAEGPLESS"
     gene            7463..>9447
                     /locus_tag="VMZ53_32940"
     CDS             7463..>9447
                     /locus_tag="VMZ53_32940"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS33371.1"
                     /translation="MNRHNHALTESERARIEQIDKLVVQGGVDELIGALSDTSWTVRR
                     AIVAGLASLGDDAVPRLARWLRDSRSSEAAIAAAVDAMSASIGTSVTNEALQLLLHAN
                     PAVAADGAQILGRRRAAEATTRLGELVHHRDDNVAVAAIEALGAIGGGAAVDALISVV
                     ESRKFFRTFPAIQVLARTGDPRAVAPLTALLADETYRLEVVRALGRTGSAHAIGPILS
                     LLKSPSDAIVRLVALSLADLLERADWAGSAEAISATLKDLLAPWLGRFAAALRGADPA
                     ERTAIAHVLGRAGDLSVLPAITAMLDDTATAPAATDALAYLGRTSDEALLAALRLDDT
                     PKRIAILPLVRSKSAAVTLRTLMNDEDPEVRARTCEALARIGDTASVPLMFDALGDPS
                     PRVAHAAISAILTLGSGETEALALGAAQSPRPAIRRHAIRLLGSFGYASAFETLVAAT
                     NDPDRRVAELATVGLGLIDDPRVEPELARLARSGDASVRAAVMRAAAHRESPASHQLL
                     ANGIADADPWVRYYATQGIGRLGSNDADASQLVVGRVQDPAPQVRIAAIEALSRIGSD
                     SAWDAVCKAAASSDPDERRAGLVGVGLQARKGASAILIDAASSSDHATRLVAIAALAR
                     VREPEALAAIDQAIGDSNAEIRDAALSLITERDDESAA"
BASE COUNT         1652 a         3142 c         3184 g         1469 t
ORIGIN      
        1 cctcgtcatc aacggcgacc acgtcaagat cagcgcgacg aaggatccgg ccgcgatcgg
       61 ctggaaggac ctcggcgtcg acatcgtgat cgagtcgacg ggcgtgttcg tcgacaaggc
      121 cggcgccggc aagcacattc aaggcggcgc gaagaaagtc atcatcagcg cgcccgcgaa
      181 ggagcccgac gtcacgctct gcatgggcat caacaccgag atgtacaagc cgggcgagca
      241 ccacgtgatc tcgaacgcgt cgtgcacgac gaactgcctc gcgcccgtcg ccaaagttct
      301 gcacgagacg ttcggcatcg tcagcggcat catgacgacg atccactcgt acacgaacga
      361 ccagcagatc ctcgatctgc cgcacaagga tctgcgtcgc gcgcgcgccg ctgcgctctc
      421 gatgattccg acgacgaccg gcgcggcgaa ggcactgaag gaagtgctgc ccgcgatgaa
      481 gggcaagctc gacggcatgg cgatccgcgt cccgacgccg aacgtctcgc tcgtcgatct
      541 cactgcgcgc ctcgagaagc aggcgaccgt cgagcaggtc aacgatgcat tccgcaaggc
      601 agcgagcggc ccgctgaagg gcatcctcgc ggtcgcagaa gagccgctcg tgtcgtgcga
      661 ctacaacggc gacaagcaca gctcgacggt cgacgccgca ctcaccaccg cgatcggcga
      721 tcaggtgaag gtgctcagct ggtacgacaa cgagatgggc tacagccagc gcctgttcga
      781 cctcgcgcgc ttcgtcgggg agcgtctgta aatggcgcta ccgggcggca tcaagagcgt
      841 cgagcagctc ggcgtcgagg gcaagcgcgt cttcgtgcgc gtcgactaca acgtcccgct
      901 cgacaagaag acgaagaaga tcaccgacga cgctcgcatc cgcgcgacgc tgccgacggt
      961 gaagcacctg atcgatcgcg gtgctcgcat cgtgctcggc agccatctcg gccgtcccga
     1021 cggtcaagtg aagcccgagt tctcgctcga gcccgtcgcc gcatatctcg ccgatctctt
     1081 gaagacggat gtcgcgctcg ccgacgagcc gaccggtgat ggcgcgcgca aggtcgtcag
     1141 cgatcttcgc gacggtcaaa tcgcgatgct cgagaacctt cgcttcaatc caggcgaaga
     1201 ggcgaatgac gagacgttcg cgcgcacgct cgcgtcgtac tgcgatgtct acgtcaacga
     1261 cgcgttcggc acggcgcatc gcgcgcatgc gtcgacggcc ggcatggtga agttcgtcgc
     1321 cgaaaagggc gcgggcttcg tcatggcgaa ggagatcgag atcctgtcgc ggctgctcgg
     1381 cgacgtcgat cgcccgtacg tcgcggtgct cggcggcgcc aaagtctccg acaagatcga
     1441 agtgctcgat gcactgctcg agcgcgtgaa cgcgatcgtg atcggcggcg cgatggcgaa
     1501 cacgttcctc gaagcgcagg gcaagaacgt cggcaagagc aaggtcgaga aggacaagct
     1561 ccccatcgcg cgtaacttcc tgcgcaaagc gagcgagaag aaggtcgcgg tgcatctgcc
     1621 gacggatgtc ctcgtaggct ccggcctcga tgacacgaac gcggtgcagg cgcgcgtcga
     1681 tgcgatcccc ggcgagtcga tggcgctcga catcggtcct ggctcgatcg agctgttccg
     1741 ccggatcctc accgaagcgc ggacggtgtt ctggaatggc ccgatgggcg tgttcgagaa
     1801 accgcagtgg gctaacggca ctgtcgcagt cgcgaaggct ctcgccgaca acaagctcgc
     1861 gctgaccgtt gtgggtggag gcgattcagc tgcggcgatc gcgcaagccg gactcgcaga
     1921 taaggtctcg cacgtgtcga ccggcggtgg tgcatcgctc gagttcgtgc agggtctcga
     1981 cctaccgggt atcgccgcac ttcgcccgta atgtcgggag tgcgcgcagg cgcacaatca
     2041 cagtgttcat ctgccgcgca ccgagcgcta gcgccactgc gtttgtccgt aagtgtgtgt
     2101 tactggaccg caatgcgcag ctcacgctgg cgctgatcat gcttggtcgg cggaggtgac
     2161 gaagcgactt ttggtggtcg atgacagcaa ggtcatgcgc gacatggtcg cagcgtgcct
     2221 gcgtcctctg ggcgaggtga ccttcgagtt tgcggcgagt ggcctggagg caatcgagcg
     2281 actcgcgctc gcgaccttca acctggtcgt gctcgacctc aacatgccgg acgtcggcgg
     2341 cgtcgaggta atcgagttca ttcggtcgca ggacaagctg cgaagcttgc cgatcctagt
     2401 cgtaacaacc cgaggcgatg acgcgagccg cacccgtgtg ctcgaggcgg gagcttccgg
     2461 ttttctagcc aagccgttcg cccccgagca gatcctcacg gatgtcagag gactgctcga
     2521 gcgagctgcc ccgtgaagga tacggtcgat ctccaggagt tcgtcggcgg ctttatcgcc
     2581 gagtcggacc agcttgtcgc atcggcgaaa gctcacctgt tggagatcga gcgtgcgagc
     2641 gaagcgaatc aacccagcgc caagctcgtt cgcgagctgt tccgcgcgat gcacacgctc
     2701 aagggcctcg ctggcatgat gggcgtgcag ccgatcgtcg acatcgcgca cgcgctcgag
     2761 acggtgctgc gcagcgcaga cctcgcaggt ggccagctcg gatcgcgtgc ctgcgagctc
     2821 ggcttcatcg gtgttcgcgc gatcgccgaa cgcgttcgcg ccgtcgcgga tcacaagccc
     2881 gtcccgccgg cgcccgagtc gctgatcgac gagctcgcgc gcgtcgacgt ctcgggtaac
     2941 acgcagtccg cgccgcttcc ggtcgttgcg agctgggaca agaagctcaa cgcgagcgag
     3001 cggcaacagg tcgtcagcgc gctcgaagcc aatcgcaagg cgtacacgct catgtttgca
     3061 cccagcgacc agcacgccgc gcgtggcgtt tcgatcagct cggtgcgcgc ctcgctcggg
     3121 gcacttggtg agatcgtcaa ggttgcgcct cgttcggtgc ctgcgacgga tacggcgcct
     3181 gccgggctcg tgttcgagat cctggtggtg agcgacgcag acgtcgaagc tctcgcgagg
     3241 gctgccgcga cgacgcccgc gcaagtcgcc gccgtggaga tcgaggtctc atcggcgccc
     3301 gcgctcgagt cgttgcccgc taccgaagac gagcttacgc cgatcggccg ctcggtcgtg
     3361 cgcgtcgagc tgtctcgcct cgacgagctc caggagcagc tcagtgcgct cgtggtcacg
     3421 cggtttcggc tcgagcgcca gatcgcttcg ctctccgcgt ccggtaacga tattcgcgtg
     3481 ctgcgcgaaa tcgcagactc gcaaggccgc cagcttcgcg atctccggcg cgcgatcttg
     3541 cgcgtgcgca tggtcaggct ctccgaagtg ctcgagccgt tgacgctgct cgtgcgcagc
     3601 atggtcaagc ccgggatcaa agaagcgcgc ctggagctcg acgtccgcga cagcgagctc
     3661 gacaaagcag tcgccgatcg gctgctacca gcgctcgttc atctcgttcg caacgcggtc
     3721 gatcatgcga tcgagcccgt cgtcgagcgt gaacgcgccg gcaagtcccg cgcaggcacc
     3781 gttcgcatcg agtcgcgcga aggcgccggc aaccagctcg tgctctccgt cgtcgacgac
     3841 ggccgcggca tcgaccgcgc ggtgattgct cgccgcgcgc gcaaggagct tgccgacgac
     3901 gatgcggtcc tcgaggtgct gacggcacct ggattctcga cgcgcgagaa ggtcaccgag
     3961 acgagtggcc gcggcatcgg catggacatc gtccgccgca tcgtcgcgag cgacctcggt
     4021 ggcgagctgt cgatgacgac gaaggttggc cagggcacga cgttcacgct cgaggttccg
     4081 ctcacgatcg cgatcatcga tgtgttctcg ttcgagtgcg cgggtcagcc gttcgtggtg
     4141 ccagtcacga ccgtcgaaga aatcttcgag ctcaccgagc agcgccgcat cgacagcccg
     4201 gcagcgctgc gcggccgatt gcccgtcgcg ctgctcgagc gccgtggccg cccgcttcct
     4261 ctcgtcgagc tctccaagat gctgcgcatc tcgaccgctg aatccgcagc ctcgaaggcg
     4321 ctcgtcgtgc gccgtcagaa cgagccgctc gcatttgccg tcgaccgcat gctgggccgg
     4381 catgaagtcg tgattcgtcc gatcgaggat gtgctcgcgc gcgtgcccgg aatcgccggc
     4441 gcgacggatc tgggcgatgg ccgccccacc ctgcttctcg atctcgtcga gctcgctgcc
     4501 ggtatctccg gttggcgtac ggagctgccg tcatgaccaa gctgcacgtc gcgttcaaag
     4561 tcggcaccgc cgaatacatc atctcgagcg accacgtcgt ccatctcgag gcgtacgccg
     4621 gcgctacgcg cgttccgggc gcgcccgagt ttgttgcggg gctggtgcaa gtccgcggca
     4681 agctcgtccc ggtagtcgac ttgcgcacgc gctttggcct cgcgcccggc gagcaaacaa
     4741 tcgacagccg cgtcattgtc gtgaaggtcg gcactcggac ggccgggctg ctcgtcgatt
     4801 cggcgcgcga cgtgctgagg atcgacgaag aaaagttcga agagccgccc gagatggtca
     4861 gagaccaggc gaacggattt gttcgcgcag tcacgaccca agccaatcgc cttctgttcg
     4921 tggtggatgt tccgcgtgtc atcggagagg aaattgctca tgcgtaattc gtttggtgct
     4981 gatttgcgtc aggtcgacgc tgtcgcgagc tcgcttcgcg agaccgcgga caaggccgag
     5041 cagatcgagc tcgcagcctc tcgcctcgcc gccggcggca acgagaacgc cgcagggacc
     5101 gatcaagcgc gtgtcgcgat cgagtcgatc ctctccaaca tcgaagagac tgccgcggct
     5161 accgaagagc tgacacgctc gcaggcgggc gttgcatcgt cggctcgcga tctcatgcag
     5221 ggactcgaga cgacggccgc cggcatgcag gagctcgcca gctccgtggc cgcggttcgc
     5281 aaggatgccg acgcgctcgc gcgtaccgcc gattccacgg ctgcgacgct cgaagaatcc
     5341 gcgcggtcga tcaaggccac gtcgcagaat gcgacggagc tcgcggccgc aagccaacag
     5401 ctcgccgcaa cggcgaccga gacgacgtcg agtgtcaccg acatggcggc gcgcggcgcg
     5461 gagaacgcgt cgacgatcga agaagtcgcc acgaccgtcg agcagatgtc tgccggcatc
     5521 gcgcggatcg ccggggactc gaagcaggtc ggcgaccgcg tcacgtcgat cgcgaccggc
     5581 ctcaccgcgg tttcggcctc gctcgaagca gttgcgcgtg acgccgcaga atcggccacg
     5641 agcgtcgagg agaccgcagc cacgtccgaa gagctcgcgc gctcggtgcg cggcgtcgcg
     5701 gatctcgcga agcagctcga ggacagcgcc atcaattccg cgagcaccgt cgcggaagtc
     5761 gctgcgtccg tcgagcaggt ggctgccacg gcggagaaga atgctgccgt cgtcgacagc
     5821 accgccaccg cgatcgaaca agcggctcgc tcggcgcaga ccgtcgcgca agccgcctcg
     5881 caggtgggga ccctcgcgtc ggcgagctca tcagcggcga gccagctcga gaccgcgaca
     5941 cgcaagatcg tgggcgccgt cgaaggcacg cgcgcgcact ctgatcgcgt cttggcctct
     6001 gcccaggcag gcggggcgac gatcggccgc acgatccaag gcctcgtcaa gatccgcgaa
     6061 gccatgtccg agagcacggt cgtcatgaag gacatgggca agcgcgccga agaaatcggc
     6121 gacatcgtcc aggcgatcaa cctgattgcg gaccgcacga acctcctgtc actcaacgcg
     6181 agcatcgagg ctgcgcgggc cggcgagcac ggtcgtgggt tcgccgtgat cgcggaagaa
     6241 attcgcgcac tcgcggatcg cgccgcggcc gccgcaggtg acgtcgcgaa gatcgtgcgt
     6301 ggcctgcaag ggacggcacg cgaagcagtg tcggcgaacg ccgagggtct gcgggtggcg
     6361 gacgagggcg cccagctctc ggagaatgcg cgctcgagcc tgcaggcgat cctagatggc
     6421 gtgtcgaccg tcgaccgctc gatccgagag atcagcatct cggcaaacga gcaagcgcaa
     6481 gcggtgcaga cggtcgtcgt gacgacgacg cgcctctccg aggagggccg cgccatctct
     6541 cgagcgggcg aagagcagat gcaagcgacg cagagcctcg cgcgcgcgtc cgcagagatg
     6601 cgcacgatgg cgaagcagac gcgcgacgca acgacggagc aagcgcgtgc gcttcgcgat
     6661 ctcgtgaaga cgaaccagct gctgtccgag agcgcgcaga aggtcagccg cgcgacgacc
     6721 gagcaagcga catcagcgag ccagctcgcg cgcgcgacga cgcagctccg tgctgccacg
     6781 aaccggacga cgaacgcgat ggcggagcag cagcggagcg tgctcgccat ggcggcaaac
     6841 gcgcaggact cggcgacggc aacggcgcgc acggtgacgg caatcaccga gcaggcccgc
     6901 ggcagcgccg agattgcacg cgcgatggat ggtgcccgca agcaagctgt acagacggct
     6961 cgcgccgtcg gtgagcaggc acgcgcgtcg aaggacatcg aagcgacgag ccgcgaggtc
     7021 gcgcgcctcg ccgtcgagct cacgagcgcg acgaacgagc agagccaagc gctgcaattg
     7081 ctcgtcaaag acggcgagga agtccgacgc gtcgcccggc aaacggcacg cgcggttggc
     7141 gagcaggcgg atgcactgtc ggcgctcgcg aacgccggcc agcggcagac gtccctcgtg
     7201 caatccgtcg cacgcgcggc caacgagcag accagctcga cggagcaagt tgccaccgcg
     7261 atgcgcgacg tgcgcgggcg aacccgcgag atcgccacga cgctgacgga gcaagtgaag
     7321 agctcgacga cgacagcgca cgatgtcgcg gtgatcgcgc aggagatcgc tggactccgc
     7381 cgcgcaaacg tcgagcaagc tgcgcaggtg actgcgcttg gggcgtctct ggggggtaat
     7441 ggcgctgccg cggagggccc tcttgaatcg tcataaccac gcgctcaccg agtccgaacg
     7501 ggctcggatc gagcagatcg acaagctcgt cgttcagggc ggcgtcgatg aattgatcgg
     7561 cgcactgagc gatacgagct ggacggtgcg gcgcgcgatc gtcgcgggtc tcgcgtcgct
     7621 cggcgacgac gcggtgcctc ggctcgcgcg gtggctccgc gattcgcgca gcagcgaggc
     7681 ggcgatcgct gccgcggtcg atgcgatgtc cgcgtcgatc ggcacgagtg tcacgaacga
     7741 agcactgcag ctgttgctcc acgcgaaccc agccgtcgct gccgatgggg cgcagatcct
     7801 cggccgccgg cgcgccgcag aagcgacgac gcgccttggc gagctcgtgc atcatcgaga
     7861 tgacaacgtc gcggtcgcgg ccatcgaagc actcggcgcc atcggcggcg gtgcggcggt
     7921 tgatgcactc atcagtgtcg tcgagagccg gaagttcttc cgtacgttcc cggcgattca
     7981 ggttctcgcg cgcaccggcg atccgcgcgc cgtcgcgccg ctcactgcgt tgctcgctga
     8041 cgagacgtat cggctcgaag tcgttcgcgc gctcggccgg accggtagtg cacacgcaat
     8101 tggccccatc ctctcgctgc tcaagagtcc aagcgatgcg atcgtgcgcc tcgtcgcgct
     8161 gtcgctggcg gacttgctcg agcgcgcaga ctgggccggt agcgccgagg cgatctccgc
     8221 aacgctcaag gacttgctcg cgccgtggct cggccgcttc gcagcggcgc tgcgtggagc
     8281 ggatccagca gagcgcaccg cgatcgcgca cgtgctcggg cgcgccggcg atctgtccgt
     8341 gctgccagcc atcaccgcga tgctcgacga tacggcgacc gcgcctgctg ccacggatgc
     8401 actcgcgtat cttggccgga cttccgacga agcgctgctc gcggcgctcc gcctcgacga
     8461 caccccgaag cgcatcgcga ttcttccgct cgtgcgctcg aagtcggcgg ccgtcacgct
     8521 gcgcacgctg atgaacgacg aagaccccga ggtgcgggcg cgcacgtgcg aggcgcttgc
     8581 tcgcatcgga gataccgcaa gcgttccact gatgttcgac gcgctcggcg atcccagccc
     8641 tcgcgtggct catgccgcaa tctccgcgat cctgacgctc ggcagcggcg agaccgaagc
     8701 cctcgcgctc ggtgccgccc agtcgccgcg ccctgcgata cgccggcatg cgattcgcct
     8761 cctcggctcg ttcggctacg cgtccgcgtt cgagacgctc gtggccgcga ccaatgatcc
     8821 agatcgacga gtcgccgagc ttgcgacggt gggcctcggc ttgatcgacg atccgcgcgt
     8881 cgagccagag ctcgcgcggc ttgcgcgctc tggcgatgcc tccgtgcgag cggcggtcat
     8941 gcgtgcggcc gctcatcgcg agtcaccggc gtcgcatcag ctgctcgcga acggcatcgc
     9001 cgacgccgat ccatgggtcc ggtactacgc gacgcagggc atcggccgcc tcggctccaa
     9061 cgacgccgat gcctcacagc tcgtcgtcgg tcgcgttcag gatccggcgc cgcaggtgcg
     9121 aatcgctgcg atcgaagcgc tctcgcgcat cggctcggac agcgcatggg acgccgtctg
     9181 caaggccgcg gcatccagcg atcctgacga acgtcgcgca ggcctcgtcg gcgtcgggtt
     9241 gcaggctcgc aaaggcgcat cggcgatcct gatcgatgcc gcttcctcgt cggatcacgc
     9301 gactcgtctt gtcgcgatcg ccgcgctcgc acgtgtacgc gagcctgaag ccctcgcagc
     9361 catcgaccag gcgatcggcg attcgaacgc ggagattcgc gacgccgcgc tctcgctcat
     9421 tacagaacgc gacgacgaat cggcggc
//
LOCUS       DATLBZ010000668         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3688138, whole genome shotgun sequence.
ACCESSION   DATLBZ010000668 DATLBZ010000000
VERSION     DATLBZ010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688138"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="VMZ53_32945"
     CDS             complement(<1..1825)
                     /locus_tag="VMZ53_32945"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HUS33372.1"
                     /translation="MTRQRNVPLLTAIAATVAVLALLAVHLWVSALPGMDKLEGFSVD
                     ARFKMRGPRAPSTDRIVIVGLDDETHRRHPEVFQTRRGYAALIDALTKYNAKVIALDL
                     FFSSPEIFLPSDIEADVRATASDVQANPPADPTAAKLALLVGKINEQLRGDELLTAAI
                     TSSKRVFLGAHFFGGPGASAPEPGKLALARHGEAADSGRGGQRRPIHAYAVDFTMDTI
                     GAGAIGAGAVNNFRDKDGIVRRMPLSVEYGGRQYMSLGLAVALYDLGKPGDTRYLVGN
                     DTLRAGGHELPVGDAASLTLDMLGKRKFTRISAADVVDGKVAPEALANKIVYVGFTLS
                     SFDKVQTPLDNLADGVELHATLTENILGNRLMRVAGPLAAILATLALSVIVILLQLRR
                     VRRRAWVPPLASLLAFAAYVVVAQLVFSGGLIIQVATPIVMASFVLIAATVGGLATEG
                     REKAHLRAVFSQYVSRTVVDKVLADPSRMKLGGERKELTVLFSDIRGFSAFAEGMGPE
                     TLASFLGEYLTPMTDLVLESGGTLDKYIGDAVMAIWAAPVDLPDHAARACEVALKMQE
                     ALIALNKKWKTEGKPEVAIGIGINTGAMSVGNMGTPARFDYT"
     gene            complement(1825..2958)
                     /locus_tag="VMZ53_32950"
     CDS             complement(1825..2958)
                     /locus_tag="VMZ53_32950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HUS33373.1"
                     /translation="MSSAAKERVLVVDDSPQNRLVATGHLEAAGFEVVAVGSGEEALD
                     VLGKDRIDLVVLDVLMPGLGGFETCRRIRATPAMADVPVLFLTALGDREATTPAIEAG
                     GDDLLPKPFHRAELLLRARALIRQRRTTAELKQALRALAEQNEALRRLEHDKRKISQL
                     IVHDLKSPVGAVLANAELLRQDATGEAAEMIADIVTAAQQLDRTARDLLDLSRAEEGQ
                     LTPKLESFLLAEVADEVVTTMRGMARWTNVRFDVDVGRERVVADRELTRRMLQNLLHN
                     AVKHAPANSAVKIEGRVDTDGLVLRVIDGGPGVPVEDVERIFDRFVTLDEDPARSGSH
                     GLGLAFCRLAAEAHGGRIWVEDREREGVGAGACFCVRIPQPAS"
BASE COUNT          525 a         1041 c          943 g          486 t
ORIGIN      
        1 ccgtgtagtc gaatcgcgcc ggcgtgccca tgttgccgac ggacatcgcg ccggtgttga
       61 tcccgatgcc gatcgcgact tcgggcttgc cctcggtctt ccatttcttg tttagcgcga
      121 tgagcgcttc ctgcatcttg agcgcgactt cgcatgcgcg ggcggcgtga tccgggaggt
      181 cgacgggcgc cgcccagatc gccatcaccg cgtcgccgat gtacttgtcg agcgtgccgc
      241 ccgactcgag cactagatct gtcatcggcg tgaggtactc gccgaggaag ctcgcgagcg
      301 tctcgggacc catgccttcg gcgaatgccg agaagccgcg aatgtccgag aacagcacgg
      361 tcagctcttt gcgctcaccg ccgagcttca tgcgcgatgg atcggcgagg accttgtcga
      421 cgacggtgcg gctcacgtat tgcgagaaca ccgcgcgcag gtgcgccttc tcgcggcctt
      481 cggtggcgag cccgcccacc gtcgctgcga tcagcacgaa cgacgccatc acgatcggcg
      541 ttgcgacctg aatgatgagg ccgccggaga acacgagctg cgcgacgacg acgtacgccg
      601 cgaaggcgag cagcgaggcc aggggaggga cccacgctcg acgacgaacg cgccgcagct
      661 gcagcaagat cacgatcaca gagagcgcga gcgtggcgag gatggcggcg agtggtcccg
      721 cgacgcgcat gagccgattg ccgagaatgt tctccgtgag cgtggcatgc agctcgacgc
      781 catcggcgag gttgtcgagc ggcgtctgca ccttgtcgaa cgacgacagc gtgaacccga
      841 cgtagacgat cttgttcgcg agcgcttcgg gcgccacctt gccgtcgacg acatcggccg
      901 cggagatgcg cgtgaacttg cgcttgccga gcatgtcgag cgtgagcgat gcagcgtcgc
      961 cgaccggaag ctcgtggccg cctgcgcgca gcgtatcgtt gccgacgagg tagcgcgtat
     1021 cgccgggctt gccgagatcg tagagcgcga cagcgagacc gagcgacatg tattgccggc
     1081 cgccgtactc gaccgacaac ggcatgcggc gaacgatgcc gtccttgtcg cggaagttgt
     1141 tgaccgcacc agcgccgatc gcgcctgcgc cgatggtgtc catcgtgaag tcgacggcgt
     1201 acgcgtgaat cgggcggcgc tgcccaccgc ggccgctgtc tgccgcttcg ccatgacgag
     1261 cgagtgcgag cttgcccggc tcgggcgccg aggcaccagg cccaccgaag aagtgcgcac
     1321 cgaggaacac gcgcttgctg ctcgtgatcg ccgcggtcag caactcgtcg ccgcgcagct
     1381 gctcgttgat cttgccgacg aggagcgcga gcttggccgc cgtcggatcc gcgggcggat
     1441 tcgcctgcac atcgctcgcg gtcgcgcgta catccgcttc gatatccgac ggcagaaaaa
     1501 tctcgggcga gctgaagaac agatcgagcg cgatgacctt cgcgttgtat ttcgtcagcg
     1561 cgtcgatcaa ggccgcatac ccgcgccgcg tctgaaacac ttcgggatgc cgccgatgcg
     1621 tctcgtcatc gagcccgacg atcacgatgc ggtcggtcga cggcgctcgc ggcccgcgca
     1681 tcttgaatcg cgcatcgacg ctaaagccct cgagcttgtc catgccgggc agggcgctca
     1741 cccacagatg caccgcgagc aacgcgagga cagcaaccgt cgcggcgatc gcggtgagta
     1801 gtggaacgtt gcgttgacgc gtcatcacga agccggttgc gggatgcgaa cgcagaagca
     1861 ggcgcccgcg cccacgcctt cacgctcgcg atcttcgacc cagatgcggc caccgtgcgc
     1921 ttcggcggcg agccggcaaa acgccagccc gaggccatgg ctgccacttc gcgcgggatc
     1981 ctcgtcgagc gtgacgaacc gatcgaagat gcgctcgacg tcctcgaccg gcacgccagg
     2041 tccaccgtcg atcacgcgca ggaccaagcc atccgtatcg acacgcccct cgatcttcac
     2101 ggcggagttt gccggcgcgt gcttcaccgc gttgtgcagc aggttctgca acatgcgccg
     2161 tgtcagctcc cgatccgcca ccacgcgctc gcgaccgaca tccacatcaa accgcacatt
     2221 cgtccaacgc gccatcccgc gcatcgtcgt cacgacctcg tccgcgacct ccgccaacaa
     2281 gaagctttcg agcttcggcg tgagctggcc ttcctcggca cgcgacaggt cgagcagatc
     2341 acgcgccgta cgatcgagct gctgcgcagc ggtcacgata tcggcgatca tctcggccgc
     2401 ttcgccggtc gcgtcttggc gcagcaattc ggcattcgcg agcaccgcac cgacgggact
     2461 cttcaagtcg tgaacgatca gctggctgat cttgcgcttg tcgtgctcga gccggcgcag
     2521 cgcttcattc tgctcggcga gcgcacgcaa tgcctgcttg agctccgccg tcgtccggcg
     2581 ctgccggatc aacgcacgcg cccgcagcag cagctccgcc cgatgaaagg gcttgggcaa
     2641 caagtcatcg ccgcccgcct cgatcgcagg tgtggtcgcc tcgcgatcgc cgagcgccgt
     2701 gagaaacagc accggcacat ctgccatcgc aggcgttgcg cgaatacgcc ggcacgtctc
     2761 gaagccaccg agccctggca tcagcacatc gagcaccacc agatcgatac ggtccttacc
     2821 caacacgtcg agcgcctcct cgccactccc caccgcgaca acctcgaacc ccgcagcctc
     2881 caaatgcccg gtcgcgacca agcgattctg cggcgaatcg tcgacgacga gcacgcgctc
     2941 cttcgctgcg ctgctcacga cgcctcgcta tcgcgcacga acgcctcgat cgtcg
//
LOCUS       DATLBZ010000669         7880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3693255, whole genome shotgun sequence.
ACCESSION   DATLBZ010000669 DATLBZ010000000
VERSION     DATLBZ010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3693255"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..1373
                     /locus_tag="VMZ53_32955"
     CDS             474..1373
                     /locus_tag="VMZ53_32955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33374.1"
                     /translation="MSSAGCASTEVASPPDAAPIAMSPVGRYDIVTSYAFASPPPDAE
                     RVLAELQAATDGPDDPARYLVDKLIARLPDADMQELAARFAPYLAAYLQTRLDSFAPQ
                     LTPAIRSMTTRLADISRRMETVETLTIASDGTARRDVFALRFGPAVIDFTQIDPVLAS
                     ATLSGDQLTIGSHSIRMPYGTMLRAGFDHAVLPVVAPGSQDLASALGKLVDCNRFGAL
                     VAEWMGIGTPEFYARACTASLTLVAANIYAKVSALDDEAFGIEVMGIAHGEDVNRDGA
                     MDRIDEGRWNGAFGTAIFEGGRR"
     gene            1394..1945
                     /locus_tag="VMZ53_32960"
     CDS             1394..1945
                     /locus_tag="VMZ53_32960"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HUS33375.1"
                     /translation="MQNTIADYLRFENASNDKHEFVRGLIVAMTGGSINHARLVTSVA
                     VQLGSQLRGRTCEAFSSELRIRVAGDELIFYPDASVVCTKPIIDIEDKHAINNPTVIV
                     EVTSPSTAAYDRTEKFAFYQLIETLREYVIVSHAQQLVEVFRLGDDGQWALVMQAAKG
                     KLRLESVDCELDIEDLYARVTGL"
     gene            complement(1947..2213)
                     /locus_tag="VMZ53_32965"
     CDS             complement(1947..2213)
                     /locus_tag="VMZ53_32965"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HUS33376.1"
                     /translation="MALVQRRVDDSPIHRRLRVTLARARGPDQDWIFVRPGFMTSGKP
                     RGKWRAADDGSIVGGMICRADVATFMLQQIESTQWLRRRPVVVW"
     gene            complement(2156..2602)
                     /locus_tag="VMZ53_32970"
     CDS             complement(2156..2602)
                     /locus_tag="VMZ53_32970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33377.1"
                     /translation="MTALRVPTLGALGLVTVLTGGLAATKLVRHCPHAAGSPELVEPP
                     AADEPTIETQARLHDPVELSGCEAPHIRNVVLRGQFIAHRTEVVDHFAIQLPGRTGSY
                     SAITDSRGYFEMRIPREDFEGDLCNLDWWHSFSDESMTLQYIVDFE"
     gene            2711..3064
                     /locus_tag="VMZ53_32975"
     CDS             2711..3064
                     /locus_tag="VMZ53_32975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HUS33378.1"
                     /translation="MVIRLSDEKKPPGPKRDGERQTGLVLDERTRTKKPPMYKVLLHN
                     DDYTTREFVVWVLQTVFHKNESDAIAIMSHVHNNGVGIAGVYTFEVAETKVTKTMALA
                     KAQQFPLQLSIEPTD"
     gene            3140..5455
                     /gene="clpA"
                     /locus_tag="VMZ53_32980"
     CDS             3140..5455
                     /gene="clpA"
                     /locus_tag="VMZ53_32980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HUS33379.1"
                     /translation="MLSPELQATLQRAVDDVRGRRHEYLTLEHLLLSVLDDPTGADII
                     TKCGGDTEKLRGELERFLEEQVDELPEGEESGPDQTLAFQRVLQRAAMHVQAAGRSQM
                     TTGNVIASMFRERDSHAVYLLEKQGITRFDVINYISHGVSKVDTGGGPVVPRTRGVED
                     TGEDDQKIANPLESFCVDLTQRAREGKIDPLIGRADELERMIQVLSRRRKNNPLLIGE
                     PGVGKTALAEGLALRIIDKAVPESLLECKVYSLDMTAVLAGTRYRGDFEERLKAVMKV
                     LIGDPKSILFIDEIHNIIGAGATSGGTMDAANILKPPLSNGELRCIGSTTFKDYRQAF
                     ERDRALSRRFQRIDVGEPTVPEAIEILKGLKSRYEEHHNVKFTDAAIEAAVELSARHI
                     SGSHLPDKAIDVIDEAGAKVRLMKPADRPDEIRPNLIEDVVAKIARIPTRSVSTADKT
                     KLQNIEGELKRLIFGQDKAIEQLATVIKLSRAGLGNPNKPTGNFLFAGPTGVGKTELA
                     KQLASVLGVEFIRFDMSEYMEKHTVSRLIGAPPGYVGFDQGGQLTDAINKHPYCVVLL
                     DEIEKAHPDIYNILLQVMDHATLTDNNGRKSDFRNVILIMSSNAGSREMALGKIGFGD
                     SIADSKDGKKALERVFTPEFRNRLDATVFFDSLPIEVIRKVARKFLTELEGQLQEKKV
                     AIEVSPAAIDWFVEHGYDKAMGARPMARLVQNTLKAPLANEILFGKLTTGGTAHVDVK
                     AGEITIECEPNDDPSDAPSTTPPAREKVTAN"
     gene            5563..7065
                     /locus_tag="VMZ53_32985"
     CDS             5563..7065
                     /locus_tag="VMZ53_32985"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HUS33380.1"
                     /translation="MTKTGLCLLALAVGGAAACEHDDYDDGDYCYGDCDPDPVPLAVD
                     DHIMIDEDQPITFAMLKANDENVNYLSKIIVPPEHGTFENGEYHPEPGYFGVDQFTYE
                     GTQEYSSYPRAKSQATAYILIKSDGIPYEHSKLVGAANADDMAVGDFNGDGKTDLVTC
                     NYNTNEMSIFINTTVAEGEYSLEKHVFEGGNSPRHLAAGDVDGDGKIDIVVATHEGVT
                     MFRNLTTQPGGLLAFDGPTKLGNASTTDVELVDLQGDGVLDLVATSEGWYPSGNLDVW
                     LNQSTPGTFAFGAKTSFSTPYTALQLMAVDADANGKPDLAILADEKLSLFLNAMTVGA
                     VTPAFQARLDRDTGPNPKSMFLADLDGDAAPELGVLHDYGDMWIYANQGATSPTFEAA
                     RVIDIQTTTELVATAEVDGDGHIDLIGAAQGTAPFELLANRSLPQQYAFESTDALIAD
                     VDTHKRASFGRPTAMVFVDLDGVPPQEIVVSLKSGNVYSSAPGLRVLYGK"
BASE COUNT         1501 a         2578 c         2546 g         1255 t
ORIGIN      
        1 cacggtaccc gtacgcgtcc tcgcggtacc cgcaggcatc gtcgcgtgtc gcgacaaccg
       61 cattgcccgc gagtcggaca cggtccccgt acgcatcgtc gcggtacccg cacgcatcgt
      121 cgcgtgtcgc gacaagcgcc atggccgcga gtcgaacacg gtacccgtac gcatcctcgc
      181 gtgtcgcgac aagcgcgttg ggcggcgagt cgaacacggt gcccgtacgc atcctcgcgt
      241 gtcgcgacaa gcgcgatggc cgcgcttcgg acacgctgtc cgagcatcgg gctcgttgga
      301 cgcgcgccgg acacacgctc gcgagaattc gccgagttgc gcgcggcacg cacgctgcaa
      361 ctgcgcgacg caccatgttg aaccatcgcc tcgcttatcg tgtctcctcg ttcacctctt
      421 cttgcgttgc tgtcgcaacc gctctcgctg tcgctaccac ggcgctcgtc gcggtgagca
      481 gcgccggctg cgcatcgacg gaggtcgcct cgccacccga tgccgcgccg atcgcgatgt
      541 cgccggtcgg acgctacgac atcgtcacgt cgtacgcgtt tgcatcaccg ccgcccgacg
      601 cagagcgcgt gctcgccgag ctgcaagccg cgaccgatgg ccccgacgat ccggcgcgtt
      661 acctcgtcga caagctcatc gcgcggctgc cagacgccga catgcaggag ctggcggcgc
      721 ggttcgcgcc gtatctcgcg gcgtatctgc agacgcgcct cgattcgttc gcgccgcagc
      781 tcacgcccgc gattcgcagc atgacgacgc ggctggcgga tatctcgcgt cgtatggaga
      841 ccgtcgagac gctgacgatc gcgagtgatg ggaccgcgcg ccgcgatgtg ttcgcgctgc
      901 ggtttggccc cgcggtgatc gacttcacgc agatcgatcc agtgctcgcg agcgcgacgt
      961 tgtccggcga tcagctgacg attggctcgc actcgattcg catgccgtac ggcacgatgt
     1021 tgcgcgcggg cttcgatcac gccgtgctgc ctgtcgtcgc gccgggctcg caggacttgg
     1081 cgagtgcgct cggcaagctc gtcgactgca atcggttcgg cgcgctcgtc gccgagtgga
     1141 tgggcatcgg cacgcccgag ttttatgcgc gtgcgtgcac cgcatcgctc acgctcgttg
     1201 cggcgaacat ctacgcgaag gtcagcgcgc tcgacgacga agcgttcggc atcgaggtga
     1261 tgggcatcgc gcacggcgag gatgtgaatc gcgacggcgc gatggatcgc atcgacgaag
     1321 gacggtggaa cggcgcattc ggcaccgcga ttttcgaggg agggcgtcga tgaaacgcgc
     1381 tacactggag aggatgcaga acacgatcgc cgactacctg cggttcgaga acgcctcgaa
     1441 cgacaagcac gagtttgtgc gtggcctgat cgtcgcgatg accggcggtt ccatcaatca
     1501 cgcgcgattg gtgacgtccg tggccgtgca actcggcagt cagttacgcg ggcgcacgtg
     1561 cgaggcgttc agttctgagc ttcggattcg cgtcgcgggc gacgagctga tcttctaccc
     1621 ggatgcgtcg gtcgtgtgca cgaaaccaat catcgacatc gaggacaagc acgcgatcaa
     1681 caatccgacc gtgatcgtcg aagtcacgag tccgtcgacc gccgcgtacg accgtaccga
     1741 gaagtttgcg ttctaccagc tgatcgagac gctgcgcgag tacgtcatcg tttcgcatgc
     1801 gcagcaactc gtcgaggtgt ttcggctcgg cgacgacggc caatgggcgc tcgtcatgca
     1861 ggcggcaaag ggcaagcttc ggctcgaatc ggtcgactgc gagctcgaca tcgaagacct
     1921 gtacgcgcgc gtcaccgggc tctagctcac cagacgacga ccggtcggcg gcgcagccac
     1981 tgcgtcgatt cgatctgttg caacatgaac gtcgcaacat cggcgcggca gatcatcccg
     2041 ccgacgatcg agccgtcgtc tgcggcgcgc catttcccgc gcggcttgcc gctcgtcatg
     2101 aagccgggcc gcacgaagat ccaatcttga tcggggccgc gggcccgagc gagcgttact
     2161 cgaagtcgac gatgtattgg agagtcatcg actcgtcgct gaacgagtgc caccagtcga
     2221 ggttgcagag atcgccttcg aagtcttcgc gcgggatccg catctcgaag tagccgcgtg
     2281 aatcggtgat cgccgagtag ctgccggtgc ggccggggag ctggatcgcg aagtgatcga
     2341 cgacctcggt ccggtgcgcg atgaactggc cacgcaggac gacgttgcgg atgtgcggcg
     2401 cctcgcagcc cgacagctcg acgggatcat gcaggcgcgc ttgcgtctcg atggtgggct
     2461 cgtcggcagc ggggggctcg actagctcgg gcgagccggc ggcatgcggg caatgtcgaa
     2521 cgagcttcgt ggccgcgagg ccgccggtca gcacggtgac gagacccaga gcaccgagag
     2581 tcgggacgcg taatgcggtc accacatcat gatagctcgc aagcgggtgg ccgacctgcc
     2641 cgaaaatggg gtgtgtccaa gagggtgacg gggactacac agaaccccgt agtctcgtaa
     2701 tattggagcc atggtgatcc gtttgtcgga tgagaaaaag ccgcccggcc cgaagcggga
     2761 cggcgagcga cagacggggc tggtgctcga cgagcggacg cgcaccaaga agcctccgat
     2821 gtacaaggtg ctgctgcaca acgacgacta cacgacgcgt gagttcgttg tctgggtgct
     2881 gcagacggtg tttcacaaga acgagagcga cgcgatcgcg atcatgagcc acgtgcacaa
     2941 caacggcgtc ggcatcgccg gcgtgtacac gttcgaggtc gccgagacga aggtcacgaa
     3001 gacgatggcg ctcgccaagg cgcagcagtt tccgctccag ctgtcgatcg aaccgacgga
     3061 ctgagctcgc cgaaccgccg gaaaccagtc gaacgactag tacccgatca gatcgcagtc
     3121 ccctacactt aggacagcca tgctctctcc cgagctccaa gccacgctgc aacgcgccgt
     3181 cgacgatgtt cgtggtcgtc gccacgagta tctgacgctc gagcatctgc tcttgtcggt
     3241 gctcgacgac ccgaccggtg ccgacatcat cacgaagtgc ggcggcgata ccgaaaagct
     3301 gcgcggcgag ctggagcgct tcctcgaaga gcaagtcgac gagctgccgg agggcgaaga
     3361 gagcgggccc gatcagacgc tcgcgttcca gcgcgttctc cagcgcgccg cgatgcacgt
     3421 gcaggcggcc ggtcgctcgc agatgaccac gggcaacgtg atcgcatcga tgtttcgcga
     3481 gcgcgactcg cacgcggtct acctgctcga gaagcagggc atcacgcgct tcgacgtcat
     3541 caactacatc tcgcacggtg tgtcgaaggt cgacaccggt ggtgggcccg tcgtgccgcg
     3601 gacgcgtggc gtcgaggaca cgggcgaaga cgatcagaag atcgcgaacc cgctcgagag
     3661 cttctgcgta gatctcacgc agcgtgcacg cgaaggcaag atcgatcccc tcatcggccg
     3721 cgctgacgag ctcgagcgca tgattcaggt gctctcgcgc cgtcggaaga acaacccgct
     3781 tctcatcggc gagccgggcg tcggcaagac ggcgctggcc gagggtctcg cgctgcggat
     3841 catcgacaag gccgtgccgg agtcgctgct cgagtgcaag gtttactcgc tcgacatgac
     3901 tgcggtgctc gcgggcacgc gctatcgcgg tgacttcgag gaacgcctca aggccgtgat
     3961 gaaggtcctc atcggcgatc cgaagtcgat cctgttcatc gacgagatcc acaacatcat
     4021 cggtgcgggc gcgacgagcg gcggcacgat ggacgccgcg aacatcctca agccgcccct
     4081 gtccaacggc gagctgcgct gcatcggctc gacgacattc aaagactacc gtcaggcgtt
     4141 cgagcgcgat cgcgcgctat cgcgccgatt ccagcgcatc gatgtcggcg agccgacggt
     4201 tcccgaagcc atcgagatcc tcaagggcct caagagccgc tacgaagagc atcacaacgt
     4261 gaagttcacg gacgcggcga tcgaggccgc tgtcgagctc tccgcccgcc acatctcggg
     4321 ctcgcatctt cccgacaagg cgatcgacgt catcgacgaa gcgggcgcca aagtccgcct
     4381 gatgaagccg gccgatcgcc ccgacgagat tcgcccgaac ctcatcgagg acgtcgtggc
     4441 gaagatcgct cgcatcccga cgcgctcggt ctcgacggca gacaagacga agctccagaa
     4501 catcgagggc gagctcaagc gcctcatctt cggtcaggac aaagcgatcg agcagctcgc
     4561 aacggtcatc aagctctcgc gcgccggcct cggcaatccg aacaagccga ccggcaactt
     4621 cctgttcgca ggcccgacgg gcgttggcaa gacggagctc gccaagcagc tcgcgagcgt
     4681 gctcggtgtc gagttcatcc gcttcgacat gtccgagtac atggagaagc acacggtctc
     4741 gcgcctcatc ggcgcgcctc ccggctacgt cggcttcgac caaggcggtc agctcaccga
     4801 cgccatcaac aagcatccgt actgtgtcgt cctcctcgac gaaatcgaga aggcgcaccc
     4861 ggacatctac aacatcctcc tccaggtgat ggaccacgcg acgctcaccg acaacaacgg
     4921 gcgcaagagc gacttccgca acgtcatcct catcatgtcg agcaacgcag gctcgcgtga
     4981 gatggcgctc ggcaagattg gcttcggcga ctcgatcgca gactccaaag acggcaagaa
     5041 ggcgctcgag cgcgtgttca cacccgagtt ccgcaatcgt ctcgacgcga cagtcttctt
     5101 cgactcgcta ccgatcgaag tcatccgcaa ggtggctcgc aagttcctca ccgagctcga
     5161 aggccagttg caggagaaga aggtcgcgat cgaggtcagc ccagccgcaa tcgattggtt
     5221 cgtcgagcac ggctacgaca aagcgatggg cgcgcgaccg atggctcgcc tcgtccagaa
     5281 cacgctgaaa gccccgctcg caaacgagat cttgttcggc aagctcacga ccggcggcac
     5341 cgcgcacgtc gacgtcaaag ccggcgagat cacgatcgag tgcgagccca acgacgatcc
     5401 gtcggacgct ccgtcgacca cgccacccgc tcgcgaaaaa gtgaccgcga actaagcgcg
     5461 ccaattacag ccgcgggcgc gccagcctgc cggtttgtgc ctcctccgtg tgacttgcgt
     5521 gaggaagttg cgcaattttc ggggctggag ggtcatatag ccatgacaaa gacggggctt
     5581 tgcctacttg cgctcgctgt cggtggggca gcagcgtgcg agcacgacga ctacgacgac
     5641 ggcgactact gctacggcga ctgcgacccg gatccggtcc cacttgccgt cgacgatcac
     5701 atcatgatcg acgaggacca gccgatcacg ttcgcgatgc tcaaagcgaa tgacgagaac
     5761 gttaactacc tcagcaagat catcgtgccg cccgagcacg gcacgttcga gaacggcgag
     5821 taccaccccg agccgggcta cttcggcgtc gaccagttca cgtacgaagg cacgcaggag
     5881 tactcgtcgt acccgagagc caagtcgcaa gcgaccgcat acatcctgat caagtccgac
     5941 ggcattccgt acgagcactc gaagctcgtc ggtgcggcga atgcggacga catggcggtc
     6001 ggcgacttca acggcgacgg caagaccgac ctcgtgacgt gcaactacaa cacgaacgag
     6061 atgtcgatct tcatcaacac gaccgtcgcc gagggcgagt actcgctcga gaagcacgtg
     6121 ttcgaaggcg gcaactcgcc tcgtcatctc gccgcgggcg acgtcgatgg tgacggcaag
     6181 atcgacatcg tcgtcgcgac gcacgaaggc gtgacgatgt tccgcaacct cacgacacag
     6241 cctggcggcc tgctcgcgtt cgacggccca accaagctcg gtaacgcgtc gacgacagac
     6301 gtcgagctcg tcgacctcca aggcgatggc gtcctcgacc tcgtggcgac gtccgaaggc
     6361 tggtacccct ccggcaacct cgacgtctgg ctgaaccaat cgacgcccgg aacgttcgcg
     6421 ttcggcgcga agacctcctt ctcgacgccg tacaccgcgc ttcagctcat ggcggtcgac
     6481 gccgacgcca acggcaagcc cgacctcgcc attctcgcgg acgagaagct ctcgctcttc
     6541 ctgaatgcaa tgacggtcgg cgcggtcacg cccgccttcc aagcccgcct cgatcgcgac
     6601 acgggaccga accccaagag catgttcctc gcggacctcg atggcgatgc cgcacccgag
     6661 ctcggcgtcc tccacgacta cggcgacatg tggatctacg caaaccaagg cgcgacgtcg
     6721 ccgacgttcg aagccgctcg cgtgatcgac atccagacga cgacggagct cgtggcaacc
     6781 gccgaagtcg atggcgacgg ccacatcgat ctcatcggcg ccgcgcaagg cacggctcca
     6841 ttcgagctgc tcgcgaatcg ctcgctgccg cagcagtacg cgttcgagtc gaccgacgcc
     6901 ctgatcgccg acgtggacac gcacaagcgc gcatcgttcg gccggccgac ggcgatggtg
     6961 ttcgtcgacc tcgacggcgt gccgccgcaa gagatcgtcg tctcgctgaa gagcggaaac
     7021 gtgtactcgt cggcgcctgg cctacgcgtc ctctacggca agtagtcgct gcgtcgcgtc
     7081 ggtcccgcaa cccgcggggc aagcacgcag cggcaagtcc gactgtcgcg aggtgatgca
     7141 gctgctcgcg acgagtgcgg tgaacgatgc gcccgagtcg gaagcagcaa gcagcggcgc
     7201 cgctgactgt cgcgacgagt gctgtgaacg acggcttcga ggtcggcggc aagaaagcag
     7261 cagccacgcg gaatctcgcg tgatgtgtgg cgaggccgcg ctcgggccgg cggcgaggtc
     7321 gcggcgcgcg cccgggccgt tgaaaggccg ggcgcgccgc tccccaccgg cccggcgctc
     7381 gccgcacaac cggtgggaga agatgaagcg agcggtgtgc ggtgaccgga tcgctagttg
     7441 gtcgtgacgg gccaacaagc aagtgtggcg gtgcagttgc gcgacggctg acgtgacggt
     7501 tggggagacg agatcgcgcg cttcgcacag gcgttgtgac gaggagtttc gctagcacag
     7561 cacgggcgac ggaccgttcg acgaagagga tgcgcccagt agtgctcggg cggcgctcgg
     7621 gccggcggcg aggtcgcggc gcgcgccctg gccgttgaaa ggccgggcgc gccgctcccc
     7681 gccggcccag cgctctccgc acaatcgagg agagaagagg agcgcgaggg cgacctcaga
     7741 cacgatcgta ggttggtggc gacgagacga gcgacgcgcg gctccccgcc ggccccgcgc
     7801 tcgtcgcttc ccggcggccc ggtgtgagtc gcgcaatcga gcgaagcacg aaagactgct
     7861 aggtgcggtc ccacggttgt
//
LOCUS       DATLBZ010000670        13305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7400910, whole genome shotgun sequence.
ACCESSION   DATLBZ010000670 DATLBZ010000000
VERSION     DATLBZ010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7400910"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VMZ53_32990"
     CDS             complement(<1..503)
                     /locus_tag="VMZ53_32990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HUS33381.1"
                     /translation="MDQGLEVLKRLSHRAACGADPETGDGAGILMQLPEKFFRIEGER
                     LGFDLPANRRFAIGNVFLPPDPGLRAACEGLLESAIRDEGQAVIGWRDVPVDMRYVGQ
                     VARDALPVFRQIYVRMRRVPPSAWERTLFVIRKLAENRIRERKADPSGYFHVASLSTE
                     TVIYKGLL"
     gene            631..1521
                     /locus_tag="VMZ53_32995"
     CDS             631..1521
                     /locus_tag="VMZ53_32995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33382.1"
                     /translation="MCVFLTGSLLTGCFDPPAPPMFVMHDARPDTSLLGDGFDAPDAT
                     PYPTGVWSPPMLAFTAMTDDDPTLTDDLLEMYFNRASDIYMVARTSTTSSWSTPVLVT
                     ELSSAQSETTPEISGDGLTIFFASRRTPTAGAEDIWMATRGSRMAAWNTPVHITELSS
                     TTFDAAATPSADGLSLVFYSDRSGTLEIYETTRVVASDPWSQAVLHSELTSGIGEFDP
                     MLSPDKHTLYYDTSAAGGGDLYIATRTGTIGAFVTINPITELNSPVDLEEDPWVSSDG
                     HHMFFASNRDGMEGIYESSR"
     gene            complement(1518..3782)
                     /locus_tag="VMZ53_33000"
     CDS             complement(1518..3782)
                     /locus_tag="VMZ53_33000"
                     /inference="COORDINATES: protein motif:HMM:NF025288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger family 4 domain-containing protein"
                     /protein_id="HUS33383.1"
                     /translation="MDRIRSLLLARTHVVVMDPDLVAQASTRPVRAADVEKLEDELGK
                     LGFILSLDLAMTLRRLPHQAMQELRVWIATTLAASVSARTAIPTSTRGELPCPWCGEL
                     KLVGSLDPCGHLVCKSCWESASYPNCPICHRRVTFVEPSQSSRTLTLVHLGLDPLGMA
                     KQRFQQLLAQLVRMTPADREEVEAVIDTIGPKAVTWLPRSIPIKETMALAVARLWLIS
                     PDPSAMVKQTADHVSTATDVLRVACVLMGGNAELQAPMKLGSLPRGLRRVVLEALEQL
                     ELENVVEHVMQHCRLWKRIGERLHPFEHAGKLPNAAFAFAVLRGTNLSAVSFGPALRE
                     RALRLPFVYVEDDVIKPIAWAAPIEDALRAGNPRSALARLTHRPAELLLRADHLVRTA
                     QTRQLDALQTVLKAVELAAVKGPADTLLTLASHVSRRARPWSRRVFFPHGDVLNAAWT
                     PDTRVPLRGDALAVMVGSIRRQLLVRAENRRQFPRAIIDRALVDLTVPIHERKVSRGR
                     LLWPRGSELALPDGPTLTLTLHGGKAAIELFDASWRYVETSPVEELHLEKLALAGVRH
                     AVMAVFSPKPSTVEVDTLRFDVRGGSHVAVPLSIDLSERRLRWLDVQIKETVELERAG
                     GYRAALAHVGRDFADLVSVHSRPTMWDVACIHAAARANTIYIRERDGSLTIYKRRDNE
                     SKPARLGRLMSGAADDGRTATIPTADAPTWFALMNGLALPKGSQGYALDARDIALGTE
                     LVAAGDLVSELAPR"
     gene            complement(3867..4745)
                     /locus_tag="VMZ53_33005"
     CDS             complement(3867..4745)
                     /locus_tag="VMZ53_33005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33384.1"
                     /translation="MRMLVLFAVLVPASIARADSKELTLPRSCAEVAPDGLDDAYRTR
                     AQQLLSRTLEQANLFVVERDCTQTYRIQFADENRTIILDGPGPRVSRAVDASGDLVAE
                     YAALIETQRRLRDTASTSVAVAEPPAPSYPTYAPTPSYPSYADDDEPASNDGPPRPVK
                     NLFYGRLGIGGVTNVDAGNIVAFGLRHTIETADIDLSVGVVAGVTLFGKLEILKTPTA
                     NKLAPYVGGGLSLSNTFSDYHDATGLGAELTAGVMLTPSHKWSLQADLSLPFYRVGED
                     YPAALSMSLGYGFVGH"
     gene            4752..5663
                     /locus_tag="VMZ53_33010"
     CDS             4752..5663
                     /locus_tag="VMZ53_33010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33385.1"
                     /translation="MKPRFSSTGMPDHAVFTRVSLRVHAPLGEATSMRIAVLLATCLL
                     AGCFVVPAKTTTTRELPAERSSPRMGLSRGLTVSTKAEHSNVIVSVLRKRDCHREVSR
                     VVEVTEHRRLRMGGVEDPRGAVFGALLAPVTLPISLLISGISVAANPTNRYEKRNVVR
                     VETSACTQPAERIPVEVTLASGVSFTRKTDADGGFTFRLPSGEPYRGVIRVRAETEVA
                     ELKYRRKMPAVTTVREAVASCARDASFTGALEVKVTVNPAGLPTKVDLDHADTALTTC
                     ITTQIAEARFPNEQRDMTLVMPFSLGE"
     gene            5679..5900
                     /locus_tag="VMZ53_33015"
     CDS             5679..5900
                     /locus_tag="VMZ53_33015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33386.1"
                     /translation="MACTTCQSYLGEWRSHGPTFPEGAWEPVQFEWFEVGAHGATSVM
                     QGTARCRTCGLVADFGCSMPDGYYFIEKK"
     gene            complement(5902..7623)
                     /locus_tag="VMZ53_33020"
     CDS             complement(5902..7623)
                     /locus_tag="VMZ53_33020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33387.1"
                     /translation="MHRYLVVIAIAACGNPSDPAETPDAAAPDAAVAPVFRNPVSLPD
                     DQLAKQSLQILGAHVDGASDSCGGCHGMTRQRIRYWRALSDTTMQSCLTDLAVTDQAS
                     ALQMISCARTMPDTATSDFTTKKLGIYATATRLPWFQFAFWRAYGDTAEQELAAFNSQ
                     VAMPKGDTIPPLTQEQFDIVAEWFARGVPMLDETLPVDPAPSTCTPGISGDVAAHVTA
                     MATQGWRAVNRGNMMAMYGCGTATDPKQCLGDIPLASEQTYGETWDVGAAKFRVLRDV
                     TYETSYWTRSSPDGRFIGHGVANVTGSYIYDLQRGANVTIATQYDPAFFPDNSGFVFQ
                     GGPRNVCGISVLTSNPTSIAMTEAACSKISTIGLYEHVGQGLGGGDYFAIDSQFVSDD
                     GGKETTTKDPATDFTTTGYTDFTPMIFDGTKYVTKGRIHISQPFEGDSTLSPSARLEM
                     TRVAGPNDKQIGFVLHKVVTTPTGSSYSITTPEIARYCISGGKPGFSYDERWVAYHHY
                     ETNGSANLYLMDLRTGNPIKISNMSPGQYALYPHFRSDGWIYAQVRDTNSGHEYTVAT
                     DAALLAE"
     gene            7844..8575
                     /locus_tag="VMZ53_33025"
     CDS             7844..8575
                     /locus_tag="VMZ53_33025"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33388.1"
                     /translation="MVLALAMPACMVSGRGTMAVETTPVAVYQEPPPPQAETFTARPG
                     HIWVRGRWDWRNGQWVWVSGHYERERANMYWAEGSWRQQGNQWVWVEGSWQTGAPSGG
                     GGVIVTTTTSGNGGGNWNNNNNGGGNWGNSTPPPVASSGQGGVASTSNVTVSAYPTAA
                     PPPPRVESYGAARPGFIWITGRWDWRGGNWQWVDGHWERERANQMWVSGSWQNNGGQW
                     IWVEGSWQTRAATPQGPVVRDHRTH"
     gene            complement(8698..10020)
                     /locus_tag="VMZ53_33030"
     CDS             complement(8698..10020)
                     /locus_tag="VMZ53_33030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33389.1"
                     /translation="MRRLVLASLLIPSLALAQGSGDGTPASADDAAKPAEGEKPADAA
                     DPAAKTPDAEPAEPEPAPPPEPPKDDKPAVTAHYDKGLKFESDDKSYELKLLFRNQVR
                     FESLRPTEDGSQFINNIYIARARFQAEGHVFGDADRYKMELGLGDIGSFSFLKDMFIE
                     HKVAPAPVWLRVGQWKRPFNRQEMVSDFASEFNERSIENELAGGGRSLGVALHNDYEK
                     SAEGIEWVFGMFNTFSGGSDRPSIAANCTTDAMTGKVTCINSRPTTLLADFGPTIVAR
                     AGWNSARAKGYSEADLEGGPLRYSIGAAYKLDLANFAKGKQPTLADNLSHGLEADWNV
                     KVNGLSISGGVVMMKLKSADPQYGFVLQPGLMVVPKHMQVAARFAIVTEGDRNNLEAL
                     GAFNYFVHGHQLKIASDFGILKRTGEDPITMTSDKPDIRIRVMGQLEI"
     gene            complement(10090..11493)
                     /locus_tag="VMZ53_33035"
     CDS             complement(10090..11493)
                     /locus_tag="VMZ53_33035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS33390.1"
                     /translation="MSLARSAPVQRDTPDPTSAPIRQVLNAFQGNANSSGTSTKAALD
                     ALRDVTGSTATPTNDAAAIGAATTSAAAASNAAASYAASTTDEAPPLPRPPTLPPPMD
                     GAIPATPTNVAPFSVPATPAPTPGDTSGNGVDASPYTVVPNPFNDKARDPSSTSPGAA
                     SFAETPSSIGRGDDLDESDRRAIAPVRAFNIVAFLRTTRGMAIAGGAALVLVIVLVFA
                     FTGGAKTDPKHAPVAAKKSGKSSGGATTGGTGQTGVSSGDSTDTGANQGMHLNADGTR
                     RTGESGDVNGETGNATGETGGELAGSDAAAAGSDATSTTTATTAPTGETTTTTPAHPK
                     TGGTKTGGGAKAGPTIGGKQVVLEYDNQAREAAPLPSTATKADQNAISKARNSYAAGN
                     QRLFAGDADGAIRYYKEALAYYPAYVAGYRGLGLAYSQQGDKPAALKAFRTYVSSVPS
                     AKDAGLIRKRITALGGK"
     gene            11558..>13305
                     /locus_tag="VMZ53_33040"
     CDS             11558..>13305
                     /locus_tag="VMZ53_33040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33391.1"
                     /translation="MAPDGTGGGVVGERVARADAGVGGGAANFCESSRLAGVGGGVAA
                     AVLDDSSRLATTGVASSRAPIVPLALAAPPALVETPALNEPLFDVPLVAPLLGLVELP
                     ALGPVAAPLALVAPPLALVAAPLALVAAPLALVEPPIAPLIAPLFDVLDEPAAFGATP
                     GVVVRGSPVGLIAPLLALVEPAGGLVAPPLALVAPVEVAPLIAPLFDAALGPTPAPGV
                     VARGSPVGLIEPLFDALVAVDVLVGVDVLVAVDVLVGVDALVEAAPAALDPPPLPLVD
                     PLPLGDPPPLPVGDPPPLPVGDPLPLVEPPPPPLVGGFAPCAVTGVASAFARVPPTGV
                     ASAARPSGFDDVPGCDVLVGCDVLAGCDVLAGCDVLAGCDVLAGCDVLAGCDVLVGGV
                     GLALVGDALAGADFDASGLVAPLRDTVFVVIVREPGFAPTGPVSTLGGLGASSPSVSS
                     NHASSSAGAAGAGAAGTGAAAAGDAGGAGDAGDAGDAGDAGGALDAGFDFASLFVGRF
                     ARPLGAASDFVGALAGAVVVGAVVVGAGVVGAAAVVVDDAVPALVGARLTVFAVPVAG
                     LPEPDGFVATPLAGVSA"
BASE COUNT         2025 a         4329 c         4594 g         2357 t
ORIGIN      
        1 aggaggccct tgtagatcac ggtctcggtc gagagactcg cgacgtggaa gtagcccgac
       61 ggatcggcct tgcgctcgcg gatccgattc tcggcgagct tgcggatgac gaacagcgtc
      121 cgctcccacg cgctcggcgg cacgcgccgc atgcgaacgt agatctggcg gaagacgggg
      181 agggcatcgc gcgcgacctg gccgacgtag cgcatgtcga ccggcacatc gcgccagccg
      241 atcacggctt ggccttcatc gcggatcgcg ctctcgagca agccttcgca agcggcgcgc
      301 agccccggat cgggcggcaa gaacacgttg ccgatcgcga accgacgatt cgccggcaga
      361 tcgaagccga gccgttctcc ctcgatgcgg aagaatttct cgggcagctg catcaagatg
      421 ccggcgccgt cacccgtctc cggatccgct ccgcatgctg ctcgatgcga cagccgcttg
      481 aggacctcga gtccctggtc cacgatggac ctcgaccgga cgcccttgat gtgcgcgacg
      541 aaaccaacgc cacaggcgtc ccgctcccgt tcgtacaaca tgtcgatgcg taatctaacg
      601 cggcgcgtcg tcgggttcca tcacttattg atgtgcgtat ttcttacggg atctcttctg
      661 actggctgct tcgatccgcc ggcgcccccg atgttcgtca tgcacgacgc gcggcccgat
      721 acgagcctcc tcggcgacgg cttcgacgct cccgatgcga cgccgtaccc gacgggtgtg
      781 tggagcccgc cgatgctcgc gttcacagcg atgaccgacg acgatccgac gctcaccgac
      841 gatctgctcg agatgtactt caaccgcgcg agcgacatct acatggtggc tcgcacgagc
      901 acgacgagct cgtggtcgac gccggtgctc gtgacggagc tgtcatctgc gcagtcggag
      961 acgacgcccg agatcagcgg cgatggcctg acgatcttct tcgcgtcgcg gcgcacgccg
     1021 actgccggtg ccgaagacat ctggatggcg acgcgtggat cgcgaatggc agcgtggaac
     1081 acgccggtgc acatcaccga gctcagctct acgacgttcg atgccgctgc gacgccgagc
     1141 gccgacggtc tatcgctcgt cttctattcg gatcgcagcg gcacgctcga gatctacgag
     1201 acgacgcgcg tggtcgccag tgatccgtgg tcgcaggcgg tcctgcattc cgagctgacg
     1261 agcggcatcg gcgagttcga tccgatgctg tcgccggaca agcacacgct ctattacgac
     1321 acgtcggcgg caggtggcgg tgacctctac atcgccacgc gcacgggcac gataggtgcg
     1381 ttcgtcacca tcaatccgat cacggagctg aacagccccg tcgatctcga ggaggatccg
     1441 tgggtgtcgt cggacggcca tcacatgttc ttcgcgagca atcgcgacgg catggaaggc
     1501 atctacgaat cgtcgcgcta acgaggcgcg agctcggaca ccagatcgcc cgcggcgacg
     1561 agctctgtcc cgagcgcgat gtcgcgcgcg tcgagcgcat aaccctgcga gcccttcggc
     1621 agcgccaggc cattcatcag cgcgaaccag gtcggcgcgt cggcggtggg aatcgttgca
     1681 gtgcgaccat cgtcggcggc gccggacatc aggcggccga gccgtgcggg cttggactcg
     1741 ttgtcgcgtc gcttgtagat cgtcagagag ccgtcgcgct cgcggatata gatcgtgttc
     1801 gcgcgcgcag ccgcgtggat acacgcgacg tcccacatcg tcggccgcga gtgaacgctg
     1861 accagatctg cgaagtcgcg acccacgtgt gcgagtgcgg cgcgatagcc acctgcgcgc
     1921 tcgagctcca ctgtctcttt gatctggaca tcgagccaac gcaggcgacg ctcggagaga
     1981 tcgatcgata gcggcaccgc aacgtgcgag ccaccgcgca cgtcgaagcg aagcgtgtcg
     2041 acctcgacgg tcgatggctt cggcgagaag acggccatca ccgcatggcg cacacccgcg
     2101 agcgcgagct tctcgaggtg aagctcttca accggcgatg tctcgacgta gcgccagctc
     2161 gcatcgaaca gctcgatcgc ggctttgccg ccgtgcagcg tcaacgtgag cgtcggcccg
     2221 tcggggagtg cgagctcgct gccgcgcggc cacaacagcc tcccgcgcga gactttgcgc
     2281 tcgtgaatcg gcaccgtgag atcgacgagc gcgcgatcga tgatggcgcg tggaaactgc
     2341 cggcgattct cggctcgcac gagcagctgc cggcggatcg agccgaccat cacggcgagc
     2401 gcatcgccgc ggagcggaac gcgagtgtcc ggcgtccacg ctgcgttgag cacgtcacca
     2461 tgcggaaaga acacgcgacg agaccacggc ctggcgcgcc gcgagacatg cgaggcgagg
     2521 gtgagcagcg tatccgcggg gccttttacg gcggcgagct cgacggcttt gagcacggtt
     2581 tgcagcgcgt cgagctggcg cgtctgcgcc gtgcgcacga ggtgatcggc gcgtagcagc
     2641 agctcggcgg ggcgatgcgt gaggcgcgcg agtgcagagc gtgggttgcc ggcgcgcagc
     2701 gcatcctcga tcggggctgc ccacgcgatc ggcttgatga catcgtcctc gacgtagacg
     2761 aacggcaggc gaagcgcgcg ctcgcgcaac gccggcccga acgagacggc ggagaggttc
     2821 gtaccgcgca ggacggcgaa tgcgaaggcg gcgttgggga gcttgccggc gtgctcgaac
     2881 ggatgcagtc gctcgccgat gcgcttccac aaccggcagt gctgcatcac atgctcgacg
     2941 acgttctcga gctccaactg ttcgagcgcc tcgagcacga cgcgccgcaa gccgcgtggc
     3001 agcgagccga gcttcatcgg cgcctggagc tccgcgttgc cgcccatgag aacgcacgcg
     3061 acgcgcaaca cgtcggtcgc ggtgctcacg tgatcggcgg tctgtttcac catcgcgctc
     3121 ggatcggggc tgatgagcca gaggcgcgcg actgcgagtg ccatcgtctc tttgatcgga
     3181 atcgaccgcg gcagccacgt caccgctttc gggccgatcg tatcgatcac cgcttcgacc
     3241 tcttcgcgat ccgcgggcgt catgcggacg agctgcgcga gcaattgctg aaaccgctgc
     3301 ttcgccatgc cgagcggatc gagaccgaga tgcacgagcg tgagcgtgcg cgagctctgc
     3361 gatggctcga cgaatgtgac gcggcgatgg cagatcgggc agttcggata gctcgccgac
     3421 tcccagcacg acttgcagac gaggtgaccg cacggatcga gcgagccgac gagcttcagc
     3481 tcaccgcacc acggacatgg cagctcgcct ctcgtcgacg tggggatcgc cgtccgcgca
     3541 ctgacgctag ccgcgagcgt ggtcgcgatc cacacgcgaa gctcctgcat cgcttgatga
     3601 ggtaagcggc gcagcgtcat cgcgagatcg aggctgagga tgaacccgag cttgccgagc
     3661 tcgtcctcga gcttctcgac atcggccgcg cgcaccggtc gcgtcgacgc ttgcgcgaca
     3721 agatctggat ccatcacgac gacgtgcgtg cgcgccagaa ggagcgagcg gatccgatcc
     3781 atgagaggtg ggcggagagt ggctgcacta ccgtgcgaag taggaaggaa gcacagccgg
     3841 agccgcccga cctcaaggta gcagattcaa tggccgacga atccgtagcc aagcgacatc
     3901 gagagggcgg ccgggtagtc ctcgccgacc cgataaaacg gcagagatag atccgcctgc
     3961 agcgaccact tgtgcgacgg cgtgagcatg accccggcgg tcagctcggc gcctagtcct
     4021 gtcgcgtcgt gatagtccga aaacgtgttc gagagcgaca agccgccacc gacatacggc
     4081 gccaacttgt tcgccgtcgg cgtcttgagg atctcgagct tgccaaagag ggtcacgccg
     4141 gcaacgacgc cgacagaaag gtcgatgtcg gcagtctcga tggtgtggcg caatccgaac
     4201 gcgacgatgt tgcccgcatc gacattggtc acgccgccga tgcccaagcg cccgtagaac
     4261 aggttcttca cggggcgtgg tggtccatcg ttcgatgcag gctcgtcatc gtccgcgtag
     4321 ctcgggtagc tcggcgtagg cgcgtaggtc gggtagctcg gcgccggcgg ttctgcgacg
     4381 gcgacgctcg tgctcgcggt atcgcgcaag cggcgttggg tctcgatcag cgctgcatat
     4441 tcagcgacga gatcgcccga cgcatcgacg gcgcgagaca cacgcgggcc gggaccgtcg
     4501 aggatgatcg tgcggttctc gtcggcgaat tggatgcgat acgtctgcgt gcagtcacgc
     4561 tcgacgacga acaggttcgc ttgctccagc gtgcgtgaga gcagctgctg tgctcgcgtg
     4621 cggtaagcgt cgtcgagacc atcgggcgca acctccgcgc agctgcgcgg cagcgtgagc
     4681 tccttcgaat cggcgcgggc gatcgacgcg gggacgagga cggcgaacaa cacgagcatg
     4741 cgcatgccgg tatgaagcct cggttttcgt cgacggggat gcccgatcac gcggtcttca
     4801 cgcgcgtgtc gctgcgtgtt cacgcgccgc tcggcgaggc tacgagcatg cggatcgccg
     4861 ttctcctcgc tacgtgtctg ctcgccggtt gcttcgtcgt tccagccaag acgaccacca
     4921 cgcgtgagct acctgccgag cgctcatcgc cgcggatggg actctcgcgc gggctcacgg
     4981 tctcgacaaa ggccgagcac tcgaacgtga tcgtctcggt gctgcgcaag cgcgattgcc
     5041 atcgcgaggt ctcgcgcgtc gtcgaagtga ccgagcatcg ccgcctgagg atgggcggcg
     5101 tcgaggatcc acgaggcgcg gtgttcggcg cactgctcgc gcccgtcacg ctccccatct
     5161 cgcttctcat ctcgggcatc tcggtcgcgg cgaatccgac gaatcgctac gagaagcgaa
     5221 acgtggtccg cgtcgagacg tccgcgtgca cgcagccagc ggagcgcatc ccggtcgaag
     5281 tcacgctcgc atcgggcgtg tcgttcacgc ggaagaccga cgccgatggc ggctttacgt
     5341 tccgcctgcc gtccggcgag ccgtatcgcg gcgtcattcg cgtgcgcgcc gagaccgaag
     5401 tcgcggagct gaagtatcgg cgcaagatgc ccgcggtcac gacggtgcgc gaggcggtag
     5461 cgagctgcgc gcgcgatgca tcgttcaccg gcgctctcga ggtaaaggtc accgtcaatc
     5521 ccgcggggct gccgacgaag gtcgatctcg atcacgcgga caccgcgctc acgacgtgca
     5581 tcacgacgca gatcgccgag gcgcgcttcc cgaacgagca gcgtgacatg acgctcgtga
     5641 tgccgttttc gctcggcgag tagcgtacgc ttcgcgcgat ggcgtgcacg acgtgtcagt
     5701 cgtacctcgg tgagtggcgc agtcacgggc cgacgttccc ggaaggcgcg tgggagcccg
     5761 ttcagttcga gtggttcgag gtcggagcgc acggcgcgac aagcgtcatg caaggcacgg
     5821 cgcgctgccg aacgtgcggc ctcgtcgccg acttcgggtg cagcatgccg gacggctact
     5881 acttcatcga gaagaagtga gctactcggc gagcagtgcg gcgtcggtcg cgaccgtgta
     5941 ctcgtggccg ctgttcgtgt cgcgaacctg cgcgtagatc catccgtcgc tgcggaagtg
     6001 cggatacagc gcgtactgac cgggcgacat gttcgagatc ttgatcgggt tgcccgtgcg
     6061 cagatccatc aagtagaggt tcgccgagcc gttcgtctcg tagtggtggt acgcgaccca
     6121 gcgctcgtcg tacgagaaac caggcttgcc gcccgagatg cagtagcgcg cgatctccgg
     6181 cgtcgtgatc gagtacgagc tgccggtggg cgtggtgacg accttgtgga gcacgaagcc
     6241 gatctgcttg tcgttcgggc ccgcgacgcg cgtcatctcg aggcgcgccg aaggcgagag
     6301 cgtggagtca ccctcgaacg gctgcgagat gtggatgcgt cccttcgtga cgtacttcgt
     6361 gccgtcgaag atcatcggcg tgaagtcggt gtaacccgtc gtcgtgaagt cggtcgccgg
     6421 atccttcgtc gtcgtttcct tgccgccgtc gtcggagacg aactggctgt cgatcgcgaa
     6481 gtagtcgccg ccgccgagtc cctggccgac gtgctcgtac aggccgatcg tcgagatctt
     6541 cgagcatgca gcctcggtca ttgcgatgct cgtcgggttc gacgtcaaga cgctgatgcc
     6601 acacacgttg cgcggcccgc cttggaacac gaagcccgag ttgtccggga agaacgccgg
     6661 gtcgtattgc gtggcgatcg tgacattcgc gccgcgctgc agatcgtaga tgtacgagcc
     6721 cgtcacgttc gcgacgccgt ggccgatgaa gcgaccgtcc ggcgagctgc gcgtccaata
     6781 cgacgtctcg taggtcacat cgcggagcac gcgaaacttc gccgcgccga cgtcccacgt
     6841 ctcgccgtac gtctgctcgg atgcgagcgg gatatcaccg aggcactgct tcggatctgt
     6901 cgcggtgccg cagccgtaca tcgccatcat gttgccgcga ttgacggcgc gccaaccttg
     6961 ggtcgccatc gccgtgacat gtgcagcgac atcacccgag atgccgggcg tgcacgtcga
     7021 cggtgctgga tcgaccggca gcgtctcgtc gagcatcggc acgccgcgcg cgaaccactc
     7081 ggcgacgatg tcgaactgct cctgcgtgag cggcgggatc gtgtcgcctt tgggcattgc
     7141 gacctgcgag ttgaacgcgg cgagctcttg ctcggccgta tcgccatacg cgcgccagaa
     7201 cgcaaactgg aaccacggca gcctcgtcgc cgtcgcgtaa atgccgagct tcttcgtcgt
     7261 aaaatcgctc gtcgcggtat cgggcatcgt gcgcgcgcag ctgatcatct gcagcgccga
     7321 tgcttggtcg gtcactgcga gatccgtcag gcagctctgc atcgtcgtgt ccgacagcgc
     7381 gcgccaatac cgaatgcgct gcctcgtcat gccgtggcaa ccgccgcacg agtccgatgc
     7441 gccatcgaca tgcgcgccga ggatctgcag cgactgcttc gcgagctgat cgtcgggcag
     7501 cgagacggga ttgcggaaca ccggcgccac cgcggcatcg ggcgcagctg cgtcaggcgt
     7561 ctccgcagga tccgacggat tgccacacgc cgcgatcgcg atcaccacga ggtagcgatg
     7621 catggcgcgc atgtttatcc gcgaaatgtc gcgtaggtac gctcaacgcc taacggttac
     7681 ttcaccagtg atgcgcccca cgcgcactgt aagcatgcga agctgcgcgc gttgacaccc
     7741 cccgggccct taggaatact gcgccgttga aggaatccgg ggacatggcc gtcctatccg
     7801 gcaaagggag agactaagaa tgctgaagac gacaaacctt ctgatggttc tcgcactcgc
     7861 gatgcccgcg tgcatggtga gtggccgcgg cacgatggct gtggagacga cgcctgtcgc
     7921 tgtgtatcaa gagccgcctc cgccacaggc tgagacgttc acggcgcgcc cgggccacat
     7981 ctgggtccgc ggtcgttggg actggcgcaa cggccagtgg gtctgggtca gcggccacta
     8041 cgagcgcgag cgcgccaaca tgtactgggc cgagggcagc tggcgccagc agggcaacca
     8101 gtgggtgtgg gtcgagggta gctggcagac cggcgcgccg agcggcggtg gtggcgtcat
     8161 cgtgacgacc acgaccagcg gcaacggcgg cggtaactgg aacaacaaca acaacggtgg
     8221 cggtaactgg ggcaactcga cgcctccgcc ggtcgcctcg agcggtcagg gcggcgttgc
     8281 ttcgacgagc aacgtgacgg tgtcggcgta cccgacggcc gcgcctcctc cgccgcgcgt
     8341 cgaatcgtac ggcgctgcac gcccgggctt catctggatc accggccgct gggattggcg
     8401 tggcggtaac tggcagtggg tcgatggcca ctgggagcgc gagcgcgcca accaaatgtg
     8461 ggtctcgggc agctggcaga acaacggtgg ccagtggatc tgggtcgagg gcagctggca
     8521 aacccgcgcg gcgacgccgc agggcccggt cgttcgcgat caccgcacgc actaaatcgg
     8581 tcgatctacc tacgcaacgc gttcgcgttg ctgcgcggct cggcatgccg agccttgccg
     8641 aaacaaacaa agcccgctgg cgcaaaccgg cgggcctttt ttatgcgctg cgctcgatca
     8701 gatttcgagc tgacccatca cgcggatacg aatgtcgggc ttgtcgctcg tcatcgtgat
     8761 cggatcttcg ccggtcctct tcaggatgcc gaagtccgac gcgatcttga gttggtgacc
     8821 gtggacgaag tagttgaacg cgccgagcgc ttcgaggttg ttgcgatcgc cctcggtcac
     8881 gatcgcgaag cgcgcggcga cctgcatgtg cttcggcacg accatgaggc cgggctgcag
     8941 cacgaagccg tactgcggat cagctgactt gagcttcatc atcaccacgc cgcccgagat
     9001 cgagagcccg ttgaccttca cgttccagtc ggcttcgagg ccgtggctca agttgtccgc
     9061 gagcgtaggc tgcttgcctt ttgcgaagtt cgcgaggtcg agcttgtacg cagcgccgat
     9121 cgagtagcgc agcgggccgc cctcgagatc cgcctcgctg tagcccttcg cgcgagcgga
     9181 gttccagcct gcgcgcgcga cgatcgttgg accgaagtcg gcgagcagcg tcgtcgggcg
     9241 gctgttgatg caggtcacct tgccggtcat cgcgtccgtc gtgcagttcg cagcgatcga
     9301 cggtcggtcc gagccgcccg aaaacgtgtt gaacatgccg aacacccact cgatgccttc
     9361 ggccgacttc tcgtaatcgt tatgcagcgc gacgccgagg ctgcggccgc caccggcgag
     9421 ctcgttctcg atcgagcgct cgttgaactc actcgcgaag tccgaaacca tctcctgtcg
     9481 attgaacggg cgcttccact gaccgacgcg tagccacacg ggcgcgggcg cgaccttgtg
     9541 ctcgatgaac atgtccttga ggaacgagaa gctgccgatg tcgccgaggc cgagctccat
     9601 cttgtagcgg tctgcatcgc cgaagacgtg tccctccgct tggaagcgcg cacgcgcaat
     9661 gtagatgttg ttgatgaact ggctgccgtc ttcggtcggg cgcagcgact cgaagcggac
     9721 ctggttgcgg aacaggagct tgagctcgta gctcttgtcg tcgctctcga acttgaggcc
     9781 cttgtcgtag tgcgcagtga ccgcgggctt gtcgtccttc ggcggctcgg gcggaggtgc
     9841 cggttcgggc tccgcgggct ccgcgtcggg cgtcttcgcc gctgggtcgg cagcgtcggc
     9901 cggcttctca ccttcggcgg gcttcgcagc gtcgtcggcg gaagccggcg tgccgtcgcc
     9961 gctgccctga gcgagggcga ggctagggat cagaagggat gcaaggacaa gtcggcgcat
    10021 ggttggagca ttttgtactc cgcgcatgtg acgccgcggt gacggcttgg agatctctgc
    10081 atgacacgtt tacttgccgc cgagcgcggt gatgcgcttg cgaatgaggc ccgcgtcctt
    10141 ggcgctcggc accgagctca cgtacgtgcg aaacgccttg agcgcggccg gcttgtcgcc
    10201 ttgctgcgaa tacgcgaggc cgagcccgcg atagccagcg acgtacgccg ggtaatacgc
    10261 gagcgcttcc ttgtagtaac gaatggcgcc gtcggcatcg cctgcaaaca gacgctggtt
    10321 gcctgcggcg tacgagttgc gcgctttcga gatcgcattc tgatcggcct tcgtcgcggt
    10381 gctcggcagc ggcgcggctt cgcgcgcttg gttgtcgtac tcgaggacga cttgcttgcc
    10441 gccgatcgtc gggcccgctt tcgcgccgcc gcccgtcttc gtgccgccgg tcttgggatg
    10501 cgcgggcgtc gtcgtcgtgg tctcgccggt cggcgccgtc gtcgcagtcg tcgtggacgt
    10561 cgcatcgctt cccgccgccg ccgcgtcgct gccggcgagc tcgccgccgg tctcgccggt
    10621 tgcattgcct gtctcgccgt tcacgtcgcc ggactctccc gtgcgacgcg ttccatcggc
    10681 gttgaggtgc atcccctgat tcgcgccggt gtccgtcgag tctccggacg atacgccggt
    10741 ctggccggtg ccgcccgtcg tcgcgccgcc gctgctcttg ccgctcttct tcgccgccac
    10801 gggggcgtgc ttcggatcgg tcttggcgcc gcccgtgaac gcgaacacga gcacgatgac
    10861 gagcacgagc gcagcgccgc ccgcgatcgc catgccacgc gtcgtgcgca agaacgcgac
    10921 gatgttgaat gcgcgcaccg gcgcgatcgc gcgacgatcg ctctcgtcga gatcatcgcc
    10981 gcggccgatc gagctcggcg tttcagcaaa gctcgcggcg ccggggctcg tcgagctcgg
    11041 atcgcgcgcc ttgtcgttga acggattcgg gacgacggtg tacgggctcg cgtcgacgcc
    11101 attgccgctc gtgtcgcccg gcgtcggggc cggcgtcgcg ggcaccgaga atggcgcgac
    11161 gtttgtcggc gtcgcaggaa tcgcgccgtc catcggcggt ggcagcgtcg gtgggcgcgg
    11221 cagcggtggc gcttcgtccg tcgtcgacgc cgcatacgat gccgccgcgt tgctcgcagc
    11281 agctgcgctc gtcgtcgccg cgccgatcgc tgcagcgtca ttggtcggcg tcgcggtcga
    11341 gccagtgaca tcgcgaagtg cgtcgagcgc ggccttcgtc gatgtgcccg agctgttcgc
    11401 gttgccctga aatgcgttga gcacctgccg aatgggcgcg gatgtcggat ccggcgtgtc
    11461 gcgctgaacc ggcgcggatc gcgcgagcga catgggcgct agcggcttcg cgcctccggt
    11521 tccaccacga ccaggcatcg gcaatccacc tcgcggcgtg gcgccggacg gaacaggcgg
    11581 cggtgtcgtc ggtgagcgcg tcgcgcgcgc ggatgctggt gtcggcggcg gggcggcgaa
    11641 tttctgcgag tcttcgcgcc tcgcgggtgt tggtggcggc gtcgccgccg cggtcctcga
    11701 cgactcctcg cgcttggcga ccaccggcgt cgcgtcttcc cgcgcgccga tcgttccgct
    11761 cgcgcttgct gcaccgcctg cgcttgtcga aacaccggcg ctcaacgagc cgctcttcga
    11821 tgtaccgctg gtcgcgccgc tgctcgggct cgtcgaattg cctgcgctcg ggcctgtcgc
    11881 agcaccgctc gcgctcgtcg caccgccgct cgcgctcgtc gcagcaccgc tcgcgctcgt
    11941 cgcagcaccg ctcgcgctcg tcgaaccgcc gatcgcgccg ctcatcgcgc cgctcttcga
    12001 tgtgctcgac gaaccggccg cgttcggcgc aacacccggg gttgtcgtgc gcggatcgcc
    12061 ggtcgggctc atcgcgccgc tgctcgcgct tgtcgaacct gcgggcgggc ttgtcgcgcc
    12121 gccgctcgcg ctcgtcgcgc ccgtcgaggt cgcgccgctc atcgcgccgc tcttcgatgc
    12181 cgcgctcggc ccaacgcctg cgccaggggt tgtcgcgcgc ggatcgcccg tcgggctcat
    12241 agaaccgctc ttcgatgcgc tcgtcgcggt cgatgtgctc gtcggagtgg acgtgctcgt
    12301 cgcagtcgat gtgctcgtcg gagtcgatgc gctcgtcgaa gccgcgccgg cggcgctcga
    12361 cccgccgccg ctgccactcg tcgatccgct gccgctcgga gatccgccgc cgctgccggt
    12421 tggcgatccg ccgccgctgc cggttggcga tccgctgccg ctcgtcgaac cgccgccgcc
    12481 gccgctcgtc ggaggtttcg cgccctgtgc agtgacgggc gtcgcgtcgg ccttcgcacg
    12541 tgtgccgccg acgggcgtcg cgtccgctgc gcgcccttcg ggcttcgacg acgtgccggg
    12601 ttgcgatgtg ctggtgggtt gcgatgtgct ggcgggttgc gacgtgctgg cgggttgcga
    12661 tgtgttggcg ggttgcgatg tgttggcggg ttgcgatgtg ctggcgggtt gcgatgtgct
    12721 ggttggaggc gtgggcttag cgctcgttgg cgacgcgctt gcgggtgccg acttcgatgc
    12781 gtcgggtttg gtcgcgccac tgcgcgacac ggtgttcgtc gtgatcgttc gcgagccggg
    12841 tttcgcgccg acggggccgg tctcgacctt gggtgggctt ggcgcgtcgt cgccatcggt
    12901 cagctcgaac catgcatcgt cgtcggcggg tgcggcgggc gcgggtgcgg cgggcacagg
    12961 ggcggcggcc gcgggggatg cgggtggcgc cggcgatgcg ggggatgcgg gggatgcggg
    13021 ggatgcgggc ggtgcgctcg atgcgggctt cgattttgcg tcgctcttcg tcggacgttt
    13081 cgcgcgacca ctcggcgctg cgtctgattt cgtcggcgcg ctcgcgggcg cggtcgtcgt
    13141 gggcgcggtc gtcgtgggcg cgggcgtcgt tggcgcggcc gcggtggtcg tcgacgatgc
    13201 ggttcctgcg ctcgtgggcg cgcgcttgac ggtcttcgcg gtcccggtcg cgggcttgcc
    13261 cgagccggac ggttttgtgg cgacgccctt ggccggcgtg tctgc
//
LOCUS       DATLBZ010000671        10404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10219515, whole genome shotgun sequence.
ACCESSION   DATLBZ010000671 DATLBZ010000000
VERSION     DATLBZ010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10219515"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VMZ53_33045"
     CDS             <1..640
                     /locus_tag="VMZ53_33045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUS33392.1"
                     /translation="SIIWATSAGDWKVIYFDTADAGIDSEREGWNATYRTGVNFNPKP
                     NQHLVDSVKGRKPGKALDVAMGQGRNAIYLASQGWKVTGIDISDEGMKIAQANAAKQK
                     LKIETINHDDMTFDFGKNRWDLVALIYAGNNHAVIEKIKASIRKGGLFVVEFFAREST
                     AGTGIGGFEQGELAALFSDGNWTIVKDEVVEDTADWGFARTKLVRFTAERKK"
     gene            complement(641..1561)
                     /locus_tag="VMZ53_33050"
     CDS             complement(641..1561)
                     /locus_tag="VMZ53_33050"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HUS33393.1"
                     /translation="MFTITRANAGELRDLDPPALAALLAVIETGSFTEAANVLRVSQP
                     AVSLAIKRLEERLGTSLLVRTRKRVAASRPGEVLAAGARQAFEALGNAVAQIENEHAE
                     PSGRVILGVHESLAAYSLPSFMARFLREYPKVELKLWNGRSMEVEAELTAGRVDLALI
                     VNASRHPDLIVVPVFDDSVELVHCIAAKRKQDPAEVLGELPVIYVPELTQSQQILAQL
                     QKRRIATPRLLPCSSLELVKSLVLDEVGVGILPRRVAEHGAHRKVYTLSPALPHYRDR
                     IALVRRFDVPTTAAIRCVIDGLAAHCKAIQ"
     gene            1611..2372
                     /locus_tag="VMZ53_33055"
     CDS             1611..2372
                     /locus_tag="VMZ53_33055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33394.1"
                     /translation="MRHLPLTTRSLLAVPAVLLAAGTLGAKPAAADTGPTYVETVVTD
                     TTTAVDLKLDSSTVLCSSADYGALFLKVLIPDLARLTLLDHQNLGAGAPCVASGQCMP
                     GNMPSNVIDAAHPTAHVLVNVKEVRADEGDPVAQTCTTTLIERVHVNIRGIDFFHERQ
                     APLGTRPFSDCATSSTDPGTGSGSGSGTDPGAGSGSGSDGSDDSAADDPYAGANDPKT
                     GGCSASVAGSSSGSAALSFGLVALGAIIARRRRRA"
     gene            complement(2686..4149)
                     /locus_tag="VMZ53_33060"
     CDS             complement(2686..4149)
                     /locus_tag="VMZ53_33060"
                     /inference="COORDINATES: protein motif:HMM:NF005858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUS33395.1"
                     /translation="MGSLFPWLAAPADSLALRVGDRSLDQRALAAACVHHVQTLQAWG
                     VAPGDRIGVWTQPALETLVAFIAHAAAGYVSVPIDPKLGDTELTHVLGNAAPRVTVAA
                     DADAVRARAGNRETFAIPMGAAASPLSQAAVDDSPALVLYTSGTTGAPKGAVITRRNI
                     ASNLDMLARAWAWSGSDIVVHALPMFHVHGLVLGLFGSQRRGGALHWVPRFTPHDLAH
                     AVREHAERAVLFAVPTMYHRLCEAAPNDATIGESLRSARLLVSGSAPLSLREHATLEQ
                     LTGQRVIERYGLTETLINCSTRHDGDRRAGVVGPPLDGVELRLVDDERRAIAADPAAI
                     GEVAVRGPHVFAGYLDNAEATQAVLDADGWFYTGDLATRHADGAIQIVGRRSTDLIKC
                     GGYKVGAGEVEAALLDHPAVAEAAVIGAPDDDLGERIVAFVVLRPDDERTTPADIVSH
                     VAMQLSPHKRPRDVKIVDALPRNAMGKVQKTKLRALL"
     gene            complement(4320..4679)
                     /locus_tag="VMZ53_33065"
     CDS             complement(4320..4679)
                     /locus_tag="VMZ53_33065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33396.1"
                     /translation="MRSLFAVAIAVIAGCAGCAGPSQQQLAETPSAREPARPYEAPPA
                     STSDKDRERLTQQFEDMETAQRAHKEANQSARQAPPPPLKGQPTPKKKGVAEQATLPP
                     KKKGVAEQAPVEQAPTP"
     gene            complement(4713..5351)
                     /locus_tag="VMZ53_33070"
     CDS             complement(4713..5351)
                     /locus_tag="VMZ53_33070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33397.1"
                     /translation="MKRLPPAPMPSLVVPPQVEASAPPAAGTGRLVVDVVDGPTQVQR
                     VRMDPQPVTTAKGRTAYRFDEKPGPLCDASPCISDLSPQNVLLGFPVIGDPDSLETEL
                     VHVDAATNIYRRSLSEYHHRSPGVYGILMTSLGGTAMLTGATLLPIGLAKDIGGMTTA
                     GAISLGAGTLALVLGIIALRHNASTYRPGSMIHFMLDAGASPGAGAAPQASP"
     gene            5470..6423
                     /locus_tag="VMZ53_33075"
     CDS             5470..6423
                     /locus_tag="VMZ53_33075"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUS33398.1"
                     /translation="MRAWFVIPLFAITACGGSLGPRGIPVLKVRGNANGFLHTNVRAN
                     VNVKVDARVRVEAPLPPPPPPAPAVELRGAPVVEFFGIPLEGAQDVVFVLDVSGSMDD
                     PAKGQLAQIAPPPPSGPQPTPSASLAVPPSAGPGPGAQPVPPPPPDPNTPPPDPNAPP
                     PDPNAQQQPTQTVETTGPRKIEVAQAELIDALSKLPAGTRMNVVFFNSNVDAIAPTIL
                     VLDEQNRPTIEAYVRETDADGRTALAPAMRVAFLLNAKRVVLLSDGLGNVNGSSEDVM
                     RDAREAVRAGVRIDTIGIGTDQDRWLLSSLANESGGLYQAF"
     gene            complement(6420..7124)
                     /locus_tag="VMZ53_33080"
     CDS             complement(6420..7124)
                     /locus_tag="VMZ53_33080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33399.1"
                     /translation="MRHVLPLVIVAIAAVTMFIVFVRARPAPQHVGAKAPPVVVAPHA
                     AQPLEQPVVSPISPRLLESGFEGMSTAEMASLDKVISAFPHFHPWLGDSRIGINGGFD
                     VVATIYFDESRGAYKVAVEKPGILTVHGLEVGRPLAEVLDLHVRHQCETISSILSSLG
                     PVYRAIRCWVGKEPARFRYVATLPDEPFGDLAAFEANTDRAEVPAGGAALEQWIRTSG
                     VQIDQILWRNDLEVER"
     gene            7219..7539
                     /locus_tag="VMZ53_33085"
     CDS             7219..7539
                     /locus_tag="VMZ53_33085"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HUS33400.1"
                     /translation="MHRARANEDVFRVIADPTRRAILDRLRAGPAPVNELAADFATSR
                     PAISKHLRVLKQSRLVTEEKVGRERLYALRPAPLQKAAAWLEGYRAFWQRNLDSLKQY
                     LENP"
     gene            7536..8300
                     /locus_tag="VMZ53_33090"
     CDS             7536..8300
                     /locus_tag="VMZ53_33090"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HUS33401.1"
                     /translation="MTKTNAARAVADTTDGVVLARVDIAVPPERVFKALTTAELTNWW
                     GQADMYKTTKFTIDLRPGGEWRTEGVGADGVPFHVGGEVIEVDAPRKLVQTWKPSWEQ
                     GPATKVSYTLEAIATGTRVTVRHTGFTNPSACSDHANGWERVFGWLSGYLEPKPERRF
                     YMVKLVPPRPSFAQDMSADERTAMQAHLGYCKSKLDAGLAIAFGPVADPSGAWGLGIL
                     SVPDEAAMRDFIENDPAIKANIGLRYESIPMINVMS"
     gene            8395..8847
                     /locus_tag="VMZ53_33095"
     CDS             8395..8847
                     /locus_tag="VMZ53_33095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33402.1"
                     /translation="MKKLIAFTLFTLVACKDKPKDTTAPESAKPAESAKPTATAPAAA
                     VDKELTDSAPLDLGKGVNVSFTKKQTVPSGENQAPTMKHTVTAKCGGKDVTVLEREDE
                     GGDAKVNVKTAGDQATFTMATHIAREGENLDETDTAVLDLATCSVTKK"
     gene            complement(8872..10239)
                     /locus_tag="VMZ53_33100"
     CDS             complement(8872..10239)
                     /locus_tag="VMZ53_33100"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS33403.1"
                     /translation="MSRRITDPPILLVEDKDSLRAMLRLTLENQGHVVIEARDQPEAI
                     EALRSGRPGLVLSDLKLNDGDGFGVLRAAKELDPDMPVIVMTAYGSIQDAVSAMKEGA
                     LDFLAKPVDPDHLLLLVERALSQRRLAAENILLKEELALRRGAPLIVGEDPKLKQVTI
                     AMHRAAASDATVLIEGESGTGKELFARAVHALSPRADGPFVAINCAAIPETLLETELF
                     GHEKGAFTGATRSRAGAFESAAGGTVFLDELGELPLELQPKLLRALEERTVKRVGGDE
                     RIPLDVRVIAASNRNLRQMVNRGTFRADLYYRLNIVRLEVPPLRERRDDIALLATNFW
                     EQLSGSHGAEPPPGLVEHLSQQDWPGNVRELRNSVERAFLMQDTELWKELESPTSSDT
                     AYDFDDSLTFRAAKERATSQWERWYVAELVQRSGGNLSKAARAAHMDRTHLRELLRKY
                     QLDKK"
BASE COUNT         1781 a         3442 c         3485 g         1696 t
ORIGIN      
        1 ctcgatcatc tgggctacga gcgcgggtga ctggaaagtc atctacttcg acacggccga
       61 cgcgggcatc gacagcgagc gtgaaggctg gaacgcgacg taccgcacgg gcgtcaactt
      121 caacccgaag ccgaaccagc accttgtcga ctccgtcaaa ggccgcaagc ccggcaaggc
      181 gctcgacgtc gcgatgggac aaggccgcaa cgcgatctat ctcgcgtcac agggttggaa
      241 agtcactggg atcgacatct cggacgaggg aatgaagatc gcccaggcga acgccgcgaa
      301 gcagaagctg aagatcgaga cgatcaacca cgacgacatg acgttcgact tcggtaagaa
      361 tcgctgggat ctcgtcgcgc tgatctacgc tggcaacaac cacgccgtca tcgagaagat
      421 caaagcgagc atccgcaagg gcggcctgtt cgtcgtcgag tttttcgcga gagagtcgac
      481 cgcgggaacg ggaatcggcg gcttcgaaca aggcgagctc gcggcgctgt tctccgacgg
      541 caattggacg atcgtcaaag acgaagtcgt cgaggacacc gcggactggg gcttcgcgag
      601 gacgaagctc gtgcgcttca ctgccgagcg caagaagtaa ctactgaatc gctttgcagt
      661 gcgctgcgag cccgtcgatc acgcagcgga tcgctgcagt ggtaggcacg tcgaagcggc
      721 gcacgagggc gatgcgatcg cgatagtgcg gcagcgctgg cgagagcgtg tacaccttgc
      781 gatgtgcgcc atgctcggcc acacgccgcg gcagaattcc gacgccgacc tcgtcgagca
      841 cgaggctctt cacgagctcg aggctcgagc acggcagaag tcgcggcgtc gcgatccgtc
      901 gcttctgtag ttgcgccaag atttgctggc tctgcgtcag ctcgggcacg tagatgactg
      961 ggagctcgcc gagcacctcc gcaggatcct gcttgcgctt ggccgcgatg cagtgaacga
     1021 gctcgacgga gtcgtcgaac acgggcacga cgatgaggtc cggatgccgg ctcgcgttca
     1081 cgatgagagc gaggtcgacg cggccggccg tgagctcggc ctcgacttcc atcgagcggc
     1141 cgttccaaag cttcagctcg accttcggat actcgcggag gaagcgcgcc atgaacgatg
     1201 gcagcgagta cgccgccagg ctctcgtgca cgccgaggat gacgcgccca cttggctcgg
     1261 cgtgctcgtt ctcgatctgc gcaaccgcat ttcccagcgc ctcgaatgcc tgcctcgcgc
     1321 ccgctgccaa gacctcgccg gggcgcgatg cggcgacgcg cttgcgcgtg cgcacgagca
     1381 gcgacgtccc gagcctttcc tcgagccgct tgatcgccaa gctgactgca ggttggctga
     1441 cgcggagtac gttcgccgcc tcggtgaagc tccctgtctc gatgacggcc aacagcgccg
     1501 cgagcgccgg cgggtcgagg tcgcggagct cgccagcatt agcgcgagtt atggtgaaca
     1561 tagtttctat tgtatacgca tattgaggtc cccgtagtac gagactggtc atgcgccacc
     1621 tgccgctgac gactcgctcg cttctcgctg ttcccgctgt cctactcgcc gccggaacgc
     1681 tcggcgccaa acccgccgcc gccgataccg gcccgaccta cgtcgagacg gtcgtgaccg
     1741 acaccacgac agccgtcgac ctcaagctcg attcgtcgac ggtgctgtgc tcgtcggccg
     1801 actacggcgc gctgttcctc aaggtcctga ttccagacct cgcgcgcctc acgctcctcg
     1861 atcaccagaa cctcggcgca ggcgccccgt gcgtggcatc aggccaatgc atgccgggca
     1921 acatgccatc caacgtgatc gacgccgcgc accccacggc gcacgtgctg gtcaacgtca
     1981 aggaagtgcg cgccgacgag ggtgaccccg tcgcgcagac ttgcacgacg acgctcatcg
     2041 agcgcgtgca cgtgaacatc cgtggcatcg acttcttcca cgagcgccag gcccctctcg
     2101 gtacgcgccc gttctccgac tgcgcgacgt cctcgaccga tccgggcacc ggctctggct
     2161 ccggctccgg caccgatccg ggcgccggct cgggctccgg ctcggacggc agcgacgact
     2221 cggcggctga tgatccgtac gcgggcgcaa acgacccgaa gacgggcggc tgcagcgcgt
     2281 cggtcgcggg ctcgtcgtct ggttccgcag cgctcagctt cggcctcgtg gcgctcggcg
     2341 cgatcatcgc tcgccgtcgc cgtcgcgcct gagcgatcac acgcgcgcgt cacacgtcgc
     2401 gctcggcgcg atcgtcgttc gccgtcgccg tcgcgcctga tcgggcgtcg cggcacagcg
     2461 ccttgaaagc gatccccgcg cctggcgcgc gcgtcgctcg cctcgccgtc gcgccgatcg
     2521 tctgtcgcgc accgcgcgtc gaaagcgatc atcgcgcgtg gcgcgcgcgt cgcccgccgt
     2581 tgccgtcgcg ccgatcgcct gtcgcggcac cgcgcgtcga aagcgaccat cgggcctggc
     2641 gcgatcatcg gccgtcgccg ccgtcgcgcc tgagcgcccg tgtcgtcaga gcagcgcgcg
     2701 gagcttggtc ttctgcacct tgcccatcgc attgcgcggc agtgcgtcga cgatcttcac
     2761 atcgcgcggc cgcttgtgcg gcgatagctg catcgcgaca tgcgagacga tgtccgcggg
     2821 tgtcgtgcgt tcgtcgtcgg ggcgcagcac cacgaacgcg acgatgcgct cgccgagatc
     2881 gtcgtcgggc gcaccgatga cggcggcttc ggcgaccgcc ggatgatcga gcagcgccgc
     2941 ctcgacctcg ccggcgccga ccttgtagcc gccgcacttg atcaaatccg tcgagcgacg
     3001 cccgacgatc tggatcgcgc cgtcggcgtg tcgcgtcgcg agatcgcccg tgtagaacca
     3061 gccgtccgcg tcgagcactg cctgcgtcgc ctcggcgttg tcgagatacc cggcgaacac
     3121 gtgtggaccg cgaaccgcga cctcgccgat cgcagcggga tccgcagcga tggcgcggcg
     3181 ctcgtcatcg acgagccgga gctccacgcc gtcgagcggc gggccgacga cacccgcgcg
     3241 tcgatcgccg tcgtgccgcg tcgagcagtt gatcagcgtc tcggtcaggc cataccgctc
     3301 gatgacgcgc tggcccgtga gctgctcgag cgtggcgtgc tcgcggaggg acagcggcgc
     3361 ggagccgctg acgagcaggc gcgcgctccg aagcgactcg ccgatcgtcg cgtcattcgg
     3421 cgctgcttcg cagagccggt gatacatcgt cgggaccgcg aacagcacgg cgcgctctgc
     3481 gtgctcgcgg acggcgtgcg cgagatcgtg tggcgtgaac cgcggcaccc agtgcagtgc
     3541 gccgccgcgg cgttggctcc cgaacaagcc gagcacgagg ccatggacat gaaacatcgg
     3601 cagcgcgtgc acgacgatgt cgctgccgct ccacgcccac gcgcgcgcga gcatgtcgag
     3661 gttgctcgca atattgcgtc gcgtgatcac ggcgcccttc ggcgcgcctg tcgtgcccga
     3721 cgtgtagagg acgagcgccg gtgaatcgtc gacggcagct tgcgaaagcg gcgacgccgc
     3781 agcacccatc gggatcgcga acgtctcgcg attgcccgca cgcgcgcgca cggcatcggc
     3841 gtccgccgcg acggtcacgc gtggtgcggc gttgccgagc acgtgcgtga gctcggtgtc
     3901 gcccagcttc ggatcgatcg gcaccgagac gtaacctgcg gccgcgtgcg cgatgaatgc
     3961 gacgagcgtc tcgagcgcgg gctgtgtcca cacgccgatg cgatcaccgg gcgcgacgcc
     4021 ccacgcctgc agcgtctgca cgtgatgcac gcaggccgcc gcgagcgcgc gttgatcgag
     4081 cgaccgatca ccgacgcgaa gtgcgagcga atcggccggc gcagcgagcc acggaaacag
     4141 cgatcccacg cgacggatct accacgcacg ccatgcggag acgcgggcgt ggcgagaccg
     4201 cgacgctcgg tcgcgctagg tgaacgcgag gcgcgcgagg cgggcgaggc gcgcgaggcg
     4261 cgtgaggcgg gtgaggcggg tgaggcgggt gagcgcgtga tgcgtgcgcg gcgagtgcgc
     4321 tatggcgttg gggcttgctc gacgggcgct tgctcggcga cgcccttctt cttcggcggc
     4381 agcgtcgctt gctcggcgac gcctttcttc ttcggcgtcg gttggccctt gagcggaggc
     4441 ggcggcgctt ggcgggcgct ctggttcgcc tctttgtgcg cgcgttgcgc ggtctccatg
     4501 tcctcgaact gctgcgtcag gcgctcgcga tccttgtcgc tcgtgctcgc gggcggcgcc
     4561 tcgtaagggc gagcgggctc acgcgccgac ggcgtttcgg caagctgctg ctgcgatggt
     4621 cccgcgcaac cggcgcaacc tgcgatgacc gcgatagcga cggcgaagag cgagcgcata
     4681 cccaacggta ccgcttacgc gaaagcgtgc aactacggtg atgcctgagg cgcggcgccg
     4741 gcgccgggac tcgcacctgc gtcgagcatg aaatggatca tcgagccggg acgataggtc
     4801 gaggcgttgt gccgcagcgc gatgatgccg agcacgagcg cgagcgtgcc cgcaccgagg
     4861 ctgatcgcgc ccgccgtcgt cattccgccg atgtctttcg cgaggccgat cgggaggagg
     4921 gtcgcgcccg tcagcatcgc ggtgccgccg agcgacgtca tcaagatgcc gtacacaccg
     4981 gggctgcggt ggtggtactc gctgagcgag cgccgataga tgttcgttgc cgcatcgacg
     5041 tggacgagct ctgtctcgag cgagtcggga tcgccgatca ccgggaagcc gagcaacacg
     5101 ttctgcggcg acagatcgct gatgcacggt gaggcgtcgc acagcgggcc gggcttttcg
     5161 tcgaagcgat aggcggtgcg gcccttggca gtcgtgacgg gttgcgggtc catgcgcacg
     5221 cgctgcacct gcgtgggacc gtcgacgaca tcgacgacca gcctgccggt tcctgccgcc
     5281 ggaggtgcgc ttgcttccac ttgaggtgga acgacgagcg acggcatcgg cgcaggtggc
     5341 aggcgcttca tgcaacctgc gattggaaga agcgctgcaa tacacagacg tagctgcatg
     5401 tgcccaccaa acacggcaga ataaggataa cgccgctctc gtcacgacgg aaacggctag
     5461 gggaaattca tgcgcgcatg gttcgtcatt ccgctgttcg cgatcaccgc ctgtggtggg
     5521 tcgctaggcc cgcgcggcat tccggtcctg aaggtccgcg gcaacgcaaa tgggttcttg
     5581 catacgaacg tgcgggcgaa cgtgaacgtg aaggtcgacg cgcgcgttcg cgtcgaggca
     5641 ccgctgccgc cgcctccgcc cccggcacct gccgtcgagc tgcgaggagc gcccgtcgtc
     5701 gagttcttcg gcatcccgct cgagggagcg caggatgtcg tgttcgtcct cgacgtctcg
     5761 ggctcgatgg atgaccccgc gaagggacag ctggcgcaga tcgctccgcc accaccgtcg
     5821 gggccgcagc cgaccccgtc cgcgtcgctc gcggtgccac cgtcggcagg gcccggtccg
     5881 ggcgctcagc ccgtcccgcc gccaccgccg gatccgaaca cgccgccgcc ggatccgaat
     5941 gcaccgccgc cggatcccaa tgcccagcag cagccgacgc agaccgtcga gaccacgggg
     6001 cctcgcaaga tcgaagtcgc acaggccgag ctcatcgacg cgctgtcgaa gctgccggca
     6061 ggcacgcgca tgaacgtcgt gttcttcaac tcgaacgtcg atgcgatcgc gccgacgatc
     6121 ctcgtgctcg acgagcagaa tcgccccacg atcgaggcgt acgtccgcga gaccgacgct
     6181 gacggccgca ccgcactcgc gccggcgatg cgcgtcgcgt tcttgctcaa cgcgaagcgc
     6241 gtcgtgctgc tgtccgacgg tctcggcaac gtgaacggca gctccgaaga cgtcatgcgt
     6301 gatgcgcgcg aagcagtgcg cgccggcgtt cgcatcgaca cgatcggcat cggcacggat
     6361 caagatcgct ggctcctctc gtcgctcgcg aacgagagtg gcggtctcta ccaggcgttc
     6421 tagcgctcga cctcgagatc gttgcgccag agaatctggt cgatctggac gcccgacgtg
     6481 cggatccact gttcgagcgc cgctccaccg gcggggactt ctgcgcgatc ggtattcgct
     6541 tcgaacgcag caagatcccc gaatggctcg tcgggcagcg tcgcgacgta gcggaaccgt
     6601 gccggctcct tgccgaccca gcagcgaatc gcgcgataca cgggtccgag cgagctgaga
     6661 atcgacgaga tcgtctcgca ctgatggcgc acgtgtagat cgagtacctc ggcgagtggc
     6721 cgtccgactt cgaggccatg caccgtcagg atcccgggtt tctcgacggc gactttgtac
     6781 gcgccgcggc tctcgtcgaa gtagatcgtc gcgacgacgt cgaagccgcc attgatgccg
     6841 atgcgcgagt cacccagcca cgggtgaaaa tgcggaaacg ccgagatgac cttgtcgagc
     6901 gacgccatct cggcggtcga cattccttcg aaaccggatt cgagcaggcg cggcgagatc
     6961 ggcgagacga caggttgctc gagaggttgg gcggcgtgcg gcgcaacgac gaccggcggt
     7021 gccttggcac cgacatgctg cggtgccggc ctcgctcgca cgaacacgat gaacatcgtc
     7081 accgctgcga tcgcgacgat gacgagcggc aacacatgtc gcatccgcat catgtcttcg
     7141 acacacggac ctcgcgcacg gttgcgcctt gacggccgcg gatcgagatg ctagatacgt
     7201 aaccaatcgg ttactgatat gcatcgtgca cgagccaacg aagatgtctt ccgcgtgatc
     7261 gcggatccga cccgcagggc gatcctggat cgtctccgcg ccggtcctgc gcccgtcaac
     7321 gagctcgctg ccgacttcgc gacgagccgg cccgcaatct cgaagcacct gcgcgtgctg
     7381 aaacagtccc gattggttac cgaagagaag gtcggtcgcg agcggctcta cgcgttgcgt
     7441 ccggcaccgc tgcagaaggc ggccgcttgg ctcgaaggct atcgcgcatt ctggcagcgc
     7501 aatctcgatt cactcaagca gtacctggag aacccatgac gaagaccaat gcggcacgcg
     7561 cggtcgcaga caccaccgac ggcgtcgtcc tcgcacgcgt cgatatcgct gttccgcccg
     7621 agcgcgtgtt caaggcgctc acgactgccg agctcaccaa ctggtggggg caagcagaca
     7681 tgtacaagac aacgaagttc acgatcgatc tgcggccggg cggagagtgg cgcaccgaag
     7741 gcgtcggcgc ggacggagtc ccgtttcacg tcggcggtga agtgatcgag gtagacgcgc
     7801 ctcgcaagct cgtgcagacg tggaagccat cgtgggagca ggggccggcg acgaaagtct
     7861 cgtacacgct cgaagcgatc gcgaccggca cgcgcgtcac cgtgcgccac accgggttca
     7921 cgaatccctc agcgtgcagc gatcacgcga acggctggga gcgcgtcttt ggctggctct
     7981 ccggctacct cgagccaaag cccgagcggc gcttctacat ggtcaagcta gtcccgccgc
     8041 gaccgagctt tgcgcaagac atgtcggccg acgagcgcac ggcgatgcaa gcgcacctcg
     8101 gctactgcaa gagcaagctc gatgcagggc tcgcgatcgc gttcggtccg gttgcagatc
     8161 cgagcggtgc gtggggcctc ggcatcttgt cggtgccgga tgaagcagcc atgcgcgact
     8221 ttatcgagaa cgatccggcg atcaaagcga acatcggcct tcgctacgag tcgattccga
     8281 tgatcaacgt gatgtcgtga ttctcgcgcg atagcggatt cacatctctg cttgcgcaat
     8341 tcgcgcagca aaagtggcac cgcgagcggt tcgattcggg gtaagcacgc gcgcatgaag
     8401 aagctcatcg cgttcacgtt gttcactctc gtcgcgtgca aggacaagcc gaaggacacg
     8461 acggcgcccg aatcggcgaa gcctgccgag tccgcgaagc cgacggcgac tgctccggcc
     8521 gcggcggtcg acaaagagct caccgatagc gcgccgctcg atctgggcaa gggcgtcaac
     8581 gtatcgttca cgaagaagca gacggtgccg tcgggcgaga atcaagcgcc gacgatgaag
     8641 cacaccgtga cggcgaagtg tggcggcaag gacgtcaccg tcctcgagcg cgaggatgaa
     8701 ggcggcgatg cgaaggtcaa cgtgaagacg gccggcgacc aggcgacgtt cacgatggct
     8761 acgcatatcg cgcgcgaagg cgagaacctc gacgagaccg acacggcggt gctcgatctc
     8821 gcgacgtgca gcgtcacgaa gaagtagtgc actgctgacg gtggcgcaga gctacttctt
     8881 gtcgagctga tacttgcgga gtagctcgcg cagatgcgtg cggtccatat gtgccgcgcg
     8941 cgccgccttc gacaaatttc cgccactgcg ctgaacgagc tctgcgacgt accagcgctc
     9001 ccactgcgaa gtggcgcgtt ccttcgcggc gcgaaacgtc agcgagtcgt cgaagtcgta
     9061 cgcggtgtcg gagctcgtcg gcgattcgag ctctttccag agctcggtgt cttgcatgag
     9121 gaacgcgcgc tcgacggagt tgcgcagctc gcgcacgttg ccgggccaat cctgttgaga
     9181 gaggtgctcg acgaggcctg gaggaggttc ggcaccatgc gagcccgaga gttgttccca
     9241 gaagttcgtc gcgaggagcg cgatgtcgtc acggcgctcg cgcagcggcg gcacttcgag
     9301 acgcacgatg ttgaggcggt agtataggtc cgcgcgaaac gtgccgcggt tcaccatctg
     9361 tcgcaggttg cgattgctcg ccgcgatgac gcgcacgtcg agcgggatgc gctcgtcacc
     9421 gcccacgcgc ttgacggttc gctcctcgag cgcgcgcaag agcttcggct gcagctcgag
     9481 tggaagctcg ccgagctcgt cgaggaacac ggtgccgccg gccgcggatt cgaacgcgcc
     9541 tgcacgcgaa cgggttgcgc cggtgaatgc gcccttctcg tgaccgaaca gctccgtctc
     9601 cagcagcgtt tccggtatgg cggcgcagtt gatcgccaca aacgggccgt ccgcccgagg
     9661 actgagcgca tgcacggcac gtgcgaacag ttcttttcca gtgccgctct cgccctcgat
     9721 caggactgtc gcatcgctcg ccgcggcgcg atgcatcgcg atagtgacct gtttcagttt
     9781 cggatcttcg ccgacgatca acggggcgcc ccggcgaagc gcgagctctt ccttcagaag
     9841 gatgttctcg gcggcgagac gtcgctgcga gagcgcgcgc tccacgagca gcagcaggtg
     9901 atcggggtcg accggtttgg cgaggaagtc gagggccccc tccttcatcg cggacacggc
     9961 atcctggatg ctgccgtacg ccgtcatgac gatgaccggc atatcggggt cgagctcttt
    10021 cgccgcgcga agaacgccga acccgtcgcc gtcattgagc ttgaggtccg agaggaccag
    10081 gcccggacgg cccgatctca gcgcttctat cgcttccggc tggtcgcgtg cctcaatgac
    10141 gacatggccc tgattctcga gcgtcagccg aagcatggcg cgcagcgagt ccttgtcctc
    10201 cacgaggagg atcggcggat cggtaatgcg acgagacact agcgaagcca gccagcgggc
    10261 acgccagcct tgcgcgcttc ttcttcgaat tccggaatgg cgcgcttatc ctgctcgacc
    10321 gctttcttca ggcggtcgag ttcgccgacc gccttctgtc ggtcggccgc aatctgacca
    10381 cgctgcgccg gatcgtcacg gttg
//
LOCUS       DATLBZ010000672         3656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8895775, whole genome shotgun sequence.
ACCESSION   DATLBZ010000672 DATLBZ010000000
VERSION     DATLBZ010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8895775"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VMZ53_33105"
     CDS             complement(<1..834)
                     /locus_tag="VMZ53_33105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33404.1"
                     /translation="MRAGRVLLISVALHGALAAVLSQMVVEGKRVAAVRPPEPAPIEV
                     AIIDSAPTLVTPTDVTTFVPAPTHSAASPTHIAPSTAHIEAGGNAREQAEQGTQVGRD
                     NETDASGNGASTSGTNGSSLMRMRGAELSLDPGTAARIEESGRRELPAETKSSGLLSH
                     EPGGKAAIYDRVTTVDVERDGTAHFHDKPDIDIKLKLPIPNLDVEEMRQDLGKLLTDW
                     YADPYASTKFGRTQDLSNINLAVPGACDEWGSIWCDDPLAPEREKYAREQKKTNGSIL
                     GG"
     gene            948..1616
                     /locus_tag="VMZ53_33110"
     CDS             948..1616
                     /locus_tag="VMZ53_33110"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS33405.1"
                     /translation="MRFVARILGVAVIAVGLPFLFGSVAGRFGAIAGIALAGLGVFWL
                     WLWLPRSAHDAFEAGKYAAADRRYRMLGVLATSAARERAALLSRSGCQAASGDVKRAD
                     ALLDRLDDKALSPAERAVWLNNRACARLAADDAQSALALVDEASALRPDVPALQHTRG
                     MALLAVGRVDDAIAVLDGMRSGGELPSHLEAERCRDLARAWTQKGQAAYAEDYRVRAE
                     ALAR"
     gene            1698..>3656
                     /locus_tag="VMZ53_33115"
     CDS             1698..>3656
                     /locus_tag="VMZ53_33115"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HUS33406.1"
                     /translation="MKYRYLIALLLAGACVAPGESETTSSVVNARTVISLTFDDTLAD
                     QVQVGDIAAARGMRAVFYINAPRLGTAGYMTKAQAIALEQQGHEIGGHTLSHANLPTL
                     SDADARTQICNDRAQLLAEGFRVTSFAYPFGADNSAVQSIVRDCGYNSARDVGGLASS
                     TYAGCQGCPWTNPVPPANLFMIRTNDSIHTDTPLSQMQQYVIDAENHGGGYVPIVFHH
                     VCDGCNPDSISRATLEAFLDWLQTRAPATQVGTVDQEIGGGVQPAVTYPPPTVSSTNL
                     AKNPSLETDADGNQVPDCWQRGGFGTNTSSYSLVTDAFDGARAQRISISSFTSGGRRL
                     VTAQDTGACAPAATVGHRYTMTAYYKSDVAPKFTVYYRNSSGTWTYLSESPSLPASST
                     YRQATYTAPAMPAGATAISVGLTIYAVGTITMDQFTLVDIDGGSPPPPPADTTPPSTQ
                     IFCNGTTCSPNPYTAPVSVSLTGWDFSGIREIRYTTNGSDPATSGIAYSSPFTVSTTT
                     TVRAIAYDNANNAMTLTKQIRFASADTTPPTLSIACNGVACSASPYTGPVSVSLAAAD
                     ASGIREIRYTTDGSNPANGTLYTAPFTVSSTTTVRAIATDNANNATTATQTIQVTAPD
                     TTPPSLSIACNGAACSSSPYSPPVSISLA"
BASE COUNT          579 a         1288 c         1164 g          625 t
ORIGIN      
        1 tccgccgagg atcgagccgt tcgtcttctt ctgctcgcgc gcgtacttct cacgctccgg
       61 tgcgagtgga tcgtcgcacc agatgctgcc ccactcgtcg cacgcgcccg gcacggcgag
      121 gttgatgttc gagagatcct gcgtgcggcc gaacttcgtc gacgcgtacg ggtccgcgta
      181 ccagtccgtg agcagcttgc cgaggtcttg ccgcatctcc tcgacgtcga ggttcgggat
      241 cgggagcttc agcttgatgt cgatgtcggg cttgtcgtgg aagtgcgcgg tgccgtcccg
      301 ctcgacgtcg acggtggtga cgcgatcgta gatcgctgct ttgccgccgg gctcgtgcga
      361 gagaagaccg ctgctcttcg tctcggccgg tagctcgcga cggccgcttt cctcgatgcg
      421 cgccgcagtt cccggatcga gcgagagctc cgcaccgcgc atgcgcatca gcgacgatcc
      481 gttcgtcccg ctcgtcgaag cgccgttgcc gctcgcgtct gtctcgttat cccttccgac
      541 ttgcgtccct tgctctgctt gctcccttgc gttgcctccg gcctcgatat gcgcagttga
      601 tggcgcaatg tgcgtaggcg acgcggcact gtgcgtaggg gccgggacga acgtcgtgac
      661 gtcggtcggg gtgacgagcg tcggcgcgga atcgatgatc gcgacttcga tcggcgccgg
      721 ttcaggcggt cgcaccgcgg cgacgcgctt tccctcgacg accatctgcg agagcacagc
      781 ggcgagcgcg ccgtgaagcg cgaccgagat gagcagcacg cgccctgccc gcatcctgct
      841 cctaggacct gcgtcaggcg ggaccggttg cccgggcatc acctcgatag caggcctcat
      901 cgaccgattg tcgcatcgct ggtgggcgac tcggttatcg ttcactcgtg cggttcgtcg
      961 cccgaatcct cggcgttgcg gtcatcgccg tcggcttgcc gttcttgttc ggcagtgtcg
     1021 caggacggtt tggcgcgatc gcgggcatcg cccttgcggg gctcggcgtg ttttggctgt
     1081 ggctttggct gccgcgcagc gcccacgacg cgttcgaggc gggcaagtac gccgcggccg
     1141 accggcgcta tcgcatgctc ggcgtccttg cgacgagcgc ggctcgcgag cgcgcagccc
     1201 tgctctctcg ctcgggttgc caagccgcga gcggggacgt gaagcgcgcc gacgcactgc
     1261 tcgatcgtct cgacgacaag gcgttgtcgc cagcggagcg tgcggtgtgg ctcaacaacc
     1321 gagcatgcgc gcgcctcgct gccgatgacg cgcagtccgc actcgcgctc gtcgacgaag
     1381 cgagcgctct gcgcccggac gtcccagcgc tgcagcacac gcgcggcatg gcgctgctgg
     1441 cagtaggacg cgtcgatgat gcgatcgcgg tgctcgacgg aatgcgctct ggcggcgagc
     1501 tgccctcgca cctcgaagcg gagcgctgcc gcgatctcgc aagagcctgg acgcaaaagg
     1561 gacaggccgc ctacgccgag gactatcggg tgcgcgccga agctctcgca cgctaacgcc
     1621 cgaatctgtc agcggctgtt catgtgcgag gtcttcgcag gcaactcagg ctgtgagatt
     1681 gcaccgcgca ttctgtcgtg aagtatcgat acctgatcgc gttgttgttg gcgggcgcct
     1741 gcgtcgcacc gggcgagagt gagaccacgt cgagcgtggt caacgcgcgg accgtcattt
     1801 cactgacgtt cgacgacacg ctcgcggatc aggtccaagt cggtgacatc gccgcggctc
     1861 gtggtatgcg cgccgtcttc tacatcaacg cgccgcgcct cgggacggcg ggctacatga
     1921 cgaaagcgca agcgatcgcg ctcgagcagc agggtcacga gatcggcggc cacacgctgt
     1981 cgcacgcgaa tctcccgacg ctctccgacg ccgacgcacg cacgcaaatt tgtaatgacc
     2041 gcgcgcagct cctcgcagag ggcttccgcg tgacgtcgtt cgcctatccg ttcggtgccg
     2101 ataactccgc cgtccaatcg atcgtgcgcg actgcggcta caactcggcg cgcgatgtcg
     2161 gcgggctcgc gtcgtcgacg tacgcggggt gtcagggatg tccgtggacc aatccggttc
     2221 cgccggcaaa cctcttcatg atccgcacga acgactcgat tcacaccgac acgccgctgt
     2281 cgcagatgca gcagtacgtc atcgacgcgg agaaccacgg cggcggctac gtgccgatcg
     2341 tgttccacca cgtgtgcgac ggctgtaatc cagactcgat cagccgcgcg acgctcgaag
     2401 cgttcctcga ttggttgcag acgcgcgcgc cggcgacgca agtcggcacg gtcgatcaag
     2461 agatcggcgg cggggtgcag cccgcggtca cgtatccgcc gccgacggtg tcgtcgacga
     2521 acctcgcgaa gaatccgtcg ctcgagaccg acgccgacgg caaccaggtt cccgactgct
     2581 ggcagcgcgg cggctttggc acgaacacgt cgagctactc gctcgtcacg gatgcgttcg
     2641 acggcgcgcg cgcgcagcgc atcagcatct cgagcttcac gtcgggcggt cgtcgcctcg
     2701 tgaccgcaca ggacaccggc gcctgcgctc cggcagccac cgtcggtcat cgctacacga
     2761 tgacggcgta ctacaaatcc gacgtcgcgc cgaagttcac ggtctactac cgcaactcga
     2821 gcggcacctg gacgtacctc tccgagagcc cgtcgctccc cgcctcgtcg acctatcgcc
     2881 aagcgacgta cacagctccg gcgatgcccg ccggcgcgac cgcgatcagc gtcggtctca
     2941 ccatctacgc ggtcggaacg atcacgatgg atcagttcac gctcgtcgac atcgacgggg
     3001 gctcgccgcc gccaccaccc gccgacacga cgccgccctc gacgcagatc ttctgcaacg
     3061 gcacgacgtg ctcgccgaat ccgtacaccg ccccggtctc ggtctcgctc acgggctggg
     3121 atttctccgg catccgcgag atccgctaca cgacgaacgg cagcgatccg gcgacgtctg
     3181 gcatcgccta cagctcgccg ttcacggtca gcacgacgac gaccgtccgc gcgatcgcat
     3241 acgacaatgc gaacaacgcg atgacgctga cgaagcagat ccgcttcgcg tccgccgaca
     3301 ccacgccgcc gacactgtcg atcgcgtgta acggcgtcgc atgctcggca tcgccgtaca
     3361 cgggcccagt ctcggtctcg ctcgccgccg ccgacgcgtc gggcattcgc gaaatccgct
     3421 acacgacgga cggcagcaac cccgccaacg gcacgctcta caccgcgccg ttcacggtga
     3481 gctcgacgac gaccgtccgc gcgatcgcga ccgacaacgc gaacaacgcg acgaccgcga
     3541 cgcaaacgat ccaagtgacc gcacccgaca cgacgccgcc ctcgctctcg atcgcgtgca
     3601 acggcgccgc ctgctcgtcg tcgccgtaca gcccaccggt ctcgatctcg ctcgcc
//
LOCUS       DATLBZ010000673         9249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3696084, whole genome shotgun sequence.
ACCESSION   DATLBZ010000673 DATLBZ010000000
VERSION     DATLBZ010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3696084"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VMZ53_33120"
     CDS             <1..621
                     /locus_tag="VMZ53_33120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33407.1"
                     /translation="ERSGRLGLELSIHSDPRRNVTGGVSITTQSTNQGSFSEGDATVT
                     VRPRDDIELELEPSMLVARGEPRFLESDAFGPRFARQDATSFGVTTRAIWTLQRDLSV
                     QAYVQALLATIRYRDAFTASADDRVIRLDALTPGAFDPAMYDGREGALNATLVGRWEY
                     RPGSTAFLVFSHAQTPLDGRAYEPSALVRGPKQDVVLLKLSWAWLR"
     gene            630..1352
                     /locus_tag="VMZ53_33125"
     CDS             630..1352
                     /locus_tag="VMZ53_33125"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HUS33408.1"
                     /translation="MPTRRDIFHLAGGAAMATLAPSAFAKGAPPPAAKPLKPGAHEPK
                     ALPFDPAKLKGLSAAMLTSHHDNNYAGAVKNLNKVELDLDKTDKDTPGYTVSSLRERE
                     LTYTNSVILHEHYFGNLGGDGKIGGTIDKRLTAQFGSAARWEELFRASAMSLGGGSGW
                     VILDANYATGDLRIYATSGHSQSVAFGQPLLVLDMFEHAYAIDFGAAHAKYIDAFFQN
                     VSWAETEKRLARADKALAALRA"
     gene            complement(1359..2699)
                     /locus_tag="VMZ53_33130"
     CDS             complement(1359..2699)
                     /locus_tag="VMZ53_33130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33409.1"
                     /translation="MKHIVQLVLGLCCVAMTLTACGDKKGADGPACTVTGEMCTADAD
                     CCTSYCEPLTGTCARVAGQCLAADDSCGAGPDCCSFSCIDFKCSGSQCTSDGGSCGAD
                     GECCGGVCTNGTCAALNPSCKTSGNTCAGNGECCSGLCSNGICNNAPSFCTQNGDTCT
                     ADTECCGGICTKANGATLGTCSVVPSSGATGCTTTGEVCGAGADYNGTDPLPMCGGEC
                     CSRACFPYGPSGVLICQPPSGCRPTGELCTKSEDCCGGPGQPDNSTSNVMCRIEPGFS
                     VGRCDNGNSCSPAGAICRLQSGSCNANANCCSGNVLQHDTCKQDNLGIPRCGIAECAP
                     NDPGCTMECADPSSHVGDICASSADCCGLPCTGSPEAGFFCQAGCQNTSQACTNDADC
                     CSGLPCVIPGGQTTGTCGSSQGCSGYGQTCDATHMCCDGLTCGTDGTCGGNIIL"
     gene            complement(2811..3983)
                     /locus_tag="VMZ53_33135"
     CDS             complement(2811..3983)
                     /locus_tag="VMZ53_33135"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HUS33410.1"
                     /translation="MSTSPLAEALRGIRVLDLSRLLPGPYLTMVLADMGADVVKIEDP
                     RVGDYLRAFPPAKGGIAGRFLAINRGKRSCALDLKAPAARDAFLQMVEKADVVVESFR
                     PGVMDKLGLSYETLAARNPKIVVCSISGFGQTGPYKDKAGHDLNYIALSGVLAMTGPA
                     GAAPQMPGIQIADLAGGALWSATSILAALVGRDRTGKGAHLDISMTEGSLALLAAELG
                     NMDCGAKPTRGTETLNGALACYGVYKTKDARYLSVGALEPKFWIALNQAIGRPPAVHE
                     LVGKPADQAKFRGELQAIFETKTAAEWTAFFADKDCLVEVVLELDELQSHPLHQARDV
                     FFEIDGGPGVGKVLQVRTPVGTPSHASAPPRLGEHTRDVLREYGLSEAQITSLAAQ"
     gene            complement(3980..4336)
                     /locus_tag="VMZ53_33140"
     CDS             complement(3980..4336)
                     /locus_tag="VMZ53_33140"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HUS33411.1"
                     /translation="MTAMPKDAQDLFDNLVPAGLKQYPDKARELNAIYCFKITGDGGG
                     EWTVDCTQDPPSCIRGDSGKAQCTVEVASDDFKTMLTDPNAGMQLYFQGKLRVSGDPM
                     LAMKLQQLFEIARSPG"
     gene            complement(4428..5366)
                     /locus_tag="VMZ53_33145"
     CDS             complement(4428..5366)
                     /locus_tag="VMZ53_33145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33412.1"
                     /translation="MRSLPMMKTALAATAFVSLVATGCVKQDGPPEEIARAIPTADQV
                     AIKLPAQGRTLGAVADYYRVTRDVTRSLNGGSAWVLILIHTIVQYPVTSVNGNVYTWG
                     PWSDTLDPAEYKLDVTANADGTFDYALSGRSKTVANSSFEEVINGHADPTPGELQGNG
                     DFSIDFDASRRVNPIDSGDAKGQVVVTYDLAARHLDMHAMGPDDNGNPASFDYAYNET
                     LDHGGDMVFDAKADIGGTTAPESLVIRSRWQSDGQGRGDARITGGDLGATQAIASECW
                     NTLFARVFYTDNASFQPTEGSVSACAYGTADLPPAQ"
     gene            complement(5431..6606)
                     /locus_tag="VMZ53_33150"
     CDS             complement(5431..6606)
                     /locus_tag="VMZ53_33150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33413.1"
                     /translation="MKRLLFVAALAACKGKAAHRFDDAAAAAPRDAASANADAGVWPE
                     LAKLPLTEAVRVITLPVKTSTPRFTVGGPVLVGDLAIVSSSQFGFIAVDFKTRQIAWT
                     KAAGSHVAPPIVIDDRVVLIGDCVTAPNVPDEDTLLGCARVVTATGQDQAYVAIHAKR
                     ELVAEFAASDGELAVFRDGDNVIWRRGEKAVAVDLLSGAAKPASADDPPLVVTYKDKT
                     WRVRRTAEGLIAAEGKPSWTTENSYGTLLGAVYIPGQSPLVRVSQPSRRYQQPELLLF
                     DIDATGSLHGQVSLHPAPGIGLVGHAINAVGDVALAVRLDTSLEREYIAGYAANALLI
                     WAYPLPPIKRADPIGIAMAPDAVVVFHDGDTLTVLPELSAPPTAPGAVRAPSENSTP"
     gene            complement(6603..7955)
                     /locus_tag="VMZ53_33155"
     CDS             complement(6603..7955)
                     /locus_tag="VMZ53_33155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33414.1"
                     /translation="MYCSIDKIDLAARVEGRPIAVQTDHRRRAEIEAEPELSSLIAMS
                     RVLNARSHLAAEGHSTAAVHYSLLDEPPLFLREALAACGGVLERARILEALGTHDEQA
                     VGDLADRCFMELASRVKLRVGSRDAAIALRMLEQQTFAAPPDRDDEPEYWRRVLELAA
                     LAGELLRAKYPGRWVQTDRAVVPFGFQVGSGTAVMFPTNRAQRVIEDGPDESLFKLLL
                     AAEETVYRNADASTSRLMPSLRHRSDIELDEVTWRPLLDDASPLDLPVVVCGVDGENT
                     FGMLRREALPKPADLAFADALDNLADEEVEVDELEVGGVALAVVTGSFYAAEKLLDRA
                     FMSTLHPLLDAELLIAATPARGCLLVTAAVDDAPSLARFASIVRSRHADAGSRAISAA
                     LLLVSRGEVAGFVRGPGDPIVEAPREGAEPRDRESTRADTVPAAPKRSGFLRRLLGRK
                     "
     gene            complement(7977..>9249)
                     /gene="glmU"
                     /locus_tag="VMZ53_33160"
     CDS             complement(7977..>9249)
                     /gene="glmU"
                     /locus_tag="VMZ53_33160"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HUS33415.1"
                     /translation="VKASLDGSFGVGSVDVALQPEQRGTGHAVQCALSTVQNEGEDRI
                     VVILSGDAPLLQSERISELVRACAEAPGGMALLSTRPDRDMPYGRLVRDAKGVLEKIV
                     EHPDATPEQRQIKDTNAGFYAIRLGHLRRDLQDLRANNAKGELYLTDLVAAAYKRGGA
                     TSIDAPFTEVSGINDRVDLSTVDAEARRRINEHWMRAGVTMVDPRSVYIDAEVGPLGK
                     DVWIGPNVALRGKTKIGDGVRIDLGSVLTDVTVADDTNIKPYSVLSESTIGERVQVGP
                     FTHCRPGSRLDDDVHIGNFVESKKTHLMAGSKANHLAYLGDASVGAKANIGAGTITCN
                     YDGVSKHKTTIEEGAFIGSDSQLVAPVTVGRGAYVGSGSTVTKDVPRGALALTRVKQV
                     NVEGWADRFREAQAKRKKNGEEAAKKPADEG"
BASE COUNT         1563 a         3043 c         3019 g         1624 t
ORIGIN      
        1 gaacgatcgg gtcgtctcgg cctcgagctc tcgatccata gcgatcctcg acgcaatgtg
       61 acggggggcg tgtcgatcac gacgcagagc acgaatcagg gaagcttctc cgagggtgac
      121 gcgaccgtca cggttcgccc gcgcgatgac atcgagctcg agctcgagcc gagcatgctc
      181 gtggcgcgcg gcgagccgcg ttttctcgag agcgatgcgt tcggcccgcg cttcgctcgc
      241 caggatgcga cgtcgtttgg cgtgacgacg cgcgcgatct ggacgctcca gcgggatctc
      301 agcgtgcaag cgtatgtgca ggcgctgctc gcaacgattc gttaccgcga cgcgttcacc
      361 gctagcgcag acgatcgggt aattcgcctg gacgcgctga cgcctggcgc gttcgatccc
      421 gcgatgtacg acggtcgcga aggtgcgctc aacgcaacgc tcgttggccg ctgggaatat
      481 cggcccggct cgacggcatt tctcgttttc tcgcacgcgc agacgccact cgacggtcgc
      541 gcgtacgaac cgagtgcgct cgtccgcggg ccgaagcaag acgtcgtctt gctgaagctc
      601 tcgtgggcct ggttgcgcta aaccagggca tgcccactcg tcgagacatc ttccatctcg
      661 cgggcggcgc ggcgatggcc acgctcgctc cgtccgcatt cgcaaagggc gcaccgccgc
      721 cggcagcgaa gccgctcaag cccggtgcgc acgagccgaa ggcgctgccg ttcgatcccg
      781 cgaagctcaa aggtttgtcg gccgcgatgc tcacgtcgca tcacgacaac aactacgcgg
      841 gcgcggtgaa gaacctcaac aaggtcgagc tcgatctcga caagaccgac aaggacacgc
      901 cgggctacac cgtctcgagc ctgcgcgagc gcgaactgac ctatacgaac tccgtcatcc
      961 tgcacgagca ctacttcggc aacctcggcg gtgatgggaa gatcggcggc acgatcgaca
     1021 agcgcctgac cgcgcagttc ggttcggccg cgcgctggga agagctgttt cgcgcgagcg
     1081 cgatgtcgct cggcggtggc agcggctggg tgatcctcga cgcgaactac gcgacgggcg
     1141 acctgcgcat ctacgcgacg agcggccaca gtcagtcggt tgcgtttggc cagccgctcc
     1201 tcgtgctcga catgttcgag catgcgtacg cgatcgattt tggcgccgcg cacgcgaagt
     1261 acatcgatgc gttctttcag aacgtgagct gggccgaaac ggaaaagcgc cttgcccggg
     1321 cggacaaggc gctggcggct ctcagagcct aggtcgcgct acagaatgat gttgccgccg
     1381 cacgtgccgt cggtgccgca cgtcaggccg tcgcaacaca tgtgcgtcgc atcgcaggtc
     1441 tggccgtagc ccgagcagcc ttgcgagcta ccgcatgtgc ccgtcgtctg accgccgggg
     1501 atgacgcacg gcaggcccga gcagcagtcg gcgtcgttcg tacacgcctg gctcgtgttc
     1561 tggcagcctg cttggcagaa gaagccagct tcgggcgagc ccgtacacgg cagaccgcag
     1621 cagtccgccg acgatgcgca gatgtcaccg acgtggctcg acggatccgc acactccatc
     1681 gtgcagcccg gatcgttcgg cgcgcactcg gcgataccgc agcgcgggat gccgaggttg
     1741 tcctgcttgc acgtgtcgtg ttgcagcacg ttgcccgagc agcagttcgc gttcgcgttg
     1801 cacgagccgc tctgcaggcg gcagatcgcg cctgccggcg agcacgagtt accgttgtcg
     1861 cagcgaccga cgctgaagcc gggctcgatg cgacacatca cgttcgacgt cgagttgtcc
     1921 ggttggcccg gaccaccaca gcagtcttcg ctcttcgtgc agagctcgcc cgtgggacga
     1981 cagcccgacg gcggctgaca gatgagcacg ccgctcggtc cgtacggaaa gcatgcgcgg
     2041 ctgcagcact cgccgccgca catcggcagc gggtccgtgc cgttgtaatc agcgccggcg
     2101 ccgcagactt cgccggtggt ggtgcagccc gtcgcgccgc tcgacggaac gacagagcat
     2161 gtgccgagcg tcgcgccgtt tgccttggtg cagatgccac cgcagcattc cgtgtcggcg
     2221 gtgcaggtgt caccgttttg ggtgcagaac gacggcgcgt tgttacagat gccgttcgag
     2281 cacaggcccg agcagcactc gccgttgcca gcgcacgtgt tacccgacgt cttgcacgac
     2341 gggttcagcg ccgcacaggt tccgttcgtg caaacgccgc cacagcattc gccgtcggcg
     2401 ccacacgagc caccgtcgct cgtgcactgg ctgccggagc atttgaagtc gatgcacgag
     2461 aacgagcagc agtcgggacc ggcgccgcat gaatcatcgg cagccaagca ttggccggcg
     2521 acgcgtgcgc acgttcccgt cagtggctcg cagtagctcg tgcagcagtc cgcatcagcg
     2581 gtgcacatct cgccggtgac ggtgcacgcg ggtccatccg cgcctttctt gtcgccgcat
     2641 gcagtgagcg tcatcgcgac gcaacagagc ccgagcacga gttgaacgat gtgcttcata
     2701 aggagtctcc tctgcttcca tagtggcgaa tcgctgcagc gaggtcgcac ggaagcctta
     2761 gaattatcgg ccgcctacat gtggcttcgc cagtttcccg gcgtgcacga ttactgcgca
     2821 gcgagcgacg tgatttgagc ctctgagagg ccgtattcgc gaagcacgtc acgcgtgtgc
     2881 tcacccaggc gcgggggtgc cgaggcatgg ctcggtgtgc cgacgggtgt gcgaacttgc
     2941 agcaccttgc cgacacctgg gccgccgtcg atctcgaaga acacgtcgcg cgcttgatgc
     3001 agcggatgcg attgcagctc gtcgagctcg agcacgactt cgacgaggca gtctttgtcc
     3061 gcgaagaacg cggtccactc ggcggctgtc ttggtctcga agatcgcctg cagctcgccg
     3121 cgaaactttg cttggtcggc aggtttgccg acgagctcgt gcaccgcggg cgggcgaccg
     3181 atcgcctggt tcagcgcgat ccagaatttc ggctcgagtg caccaaccga gagatagcgc
     3241 gcatccttcg tcttgtagac gccgtaacac gcgagcgcgc cgttgagcgt ctcggtaccg
     3301 cgcgttggct tcgcgccgca gtccatgttg ccgagctcgg cagcgaggag tgcgagtgag
     3361 ccttcggtca tcgagatgtc gaggtgcgcg cctttgccgg tgcgatcgcg accgacgaga
     3421 gctgcgagga tcgacgtcgc gctccacagt gcgccacccg cgagatcggc gatctggatg
     3481 ccgggcatct gcggcgcagc acctgcgggg cccgtcatcg cgagcacgcc tgacagcgcg
     3541 atgtaattca gatcgtgacc cgctttgtcc ttgtatggac cagtctggcc aaagccgctg
     3601 atcgagcaga cgacgatctt cggattgcgc gcggcgagcg tttcgtacga caggccgagc
     3661 ttgtccatga cgccgggtcg aaagctctcg acgacgacat cggccttctc gaccatctgc
     3721 agaaacgcgt cgcgtgcagc gggcgctttg agatcgagcg cgcacgatcg tttgccgcga
     3781 ttgatcgcga gaaaccgacc ggcgataccg cctttggcgg gaggaaacgc gcgcaggtaa
     3841 tcaccgacgc gcggatcctc gatcttgacg acgtcggcgc ccatgtcggc gagcaccatc
     3901 gtcagatacg gaccgggcaa cagacgcgac agatcgagaa ctcgaatgcc gcgcagtgcc
     3961 tccgcgagag gagacgtgct caccctgggc tgcgcgcgat ctcgaacagt tgctgcagct
     4021 tcatcgcgag catcggatca cccgagacgc ggagcttgcc ctggaagtag agctgcatgc
     4081 cggcgttggg atcggtgagc atcgtcttga agtcgtcgct cgcgacttcg accgtgcact
     4141 gcgccttgcc gctgtcgccg cggatgcagc tcggcggatc ttgcgtgcag tcgaccgtcc
     4201 actcgccgcc gccgtcaccg gtgatcttga agcagtagat cgcgttcagc tcgcgagcct
     4261 tgtccggata ctgcttcaag ccggccggga cgaggttatc gaacagatcc tgcgcgtcct
     4321 tgggcatcgc tgtcatgagt cgtttcctcc gacggcggtg ttaccccgaa acgcagaaag
     4381 cggacaaggc ctgcgcccgt ccgctttcga gctgtaacgt gcctcagtta ctgggccgga
     4441 gggaggtccg ccgtgccata cgcgcacgcc gagacgctgc cttcggtcgg ctggaagctc
     4501 gcgttgtcgg tgtagaagac gcgcgcaaac agcgtgttcc agcactccga cgcgatcgcc
     4561 tgcgttgcac cgaggtcacc gccggtgatg cgcgcatcgc cacggccctg gccgtccgac
     4621 tgccagcgcg agcggatgac gaggctctcc ggcgccgtcg tgccgccgat gtcggccttc
     4681 gcgtcgaaca ccatgtcgcc gccgtggtcg agcgtctcgt tgtacgcgta gtcgaacgac
     4741 gccgggttgc cgttgtcgtc gggacccatc gcgtgcatgt cgaggtggcg cgcggcgagg
     4801 tcgtacgtga cgacgacttg gcccttcgcg tcgcccgagt cgatcgggtt cacgcgacgg
     4861 ctcgcgtcga agtcgatcga gaagtcgccg ttgccttgga gctcgcccgg cgtcggatcg
     4921 gcgtgaccgt tgatgacctc ttcgaacgag gaattcgcga ccgtcttgct gcggcccgag
     4981 agcgcgtagt cgaacgtgcc gtccgcgttc gcggtgacgt cgagcttgta ctcggccggg
     5041 tcgagcgtgt cgctccacgg tccccacgtg taaacgttgc cgttcacgct cgtcaccgga
     5101 tactggacga tcgtgtggat gaggatgagc acccacgcgc tgccgccgtt gagcgagcgc
     5161 gtgacatcgc gcgtgacgcg gtagtagtcg gcgacagcgc cgagcgtgcg gccttgtgca
     5221 ggcagcttga tcgcgacttg atcagccgtc ggaatggcgc gcgcgatttc ctcgggcggg
     5281 ccgtcttgct ttacgcagcc ggtggcaacg agcgagacaa aggcggtggc ggcaagggcg
     5341 gtcttcatca tcggtaggct cctcatgttt tcctcgtcct gcctctagag cagctggcgt
     5401 accagccgcg tttcgaaggg gatcgagtac ttacggtgtg gagttttccg acggagcccg
     5461 aaccgcgcct ggagccgtag gaggtgccga caattcgggc aggactgtta acgtgtcgcc
     5521 gtcgtggaac accacgactg catcgggagc catcgcgatg ccgatcggat cggcgcgttt
     5581 gatcggtggg agagggtagg cccagatgag caacgcgttg gcggcgtagc cggcgatgta
     5641 ctcgcgctcg agcgatgtat cgagccgcac tgcgagcgcg acgtcgccaa ccgcgttgat
     5701 cgcgtgacca acgaggccga tgccgggagc gggatgaagc gacacctggc cgtgtaacga
     5761 gcccgttgca tcgatgtcga acaggagcag ctcgggctgt tgataccgac gcgacggctg
     5821 cgagacgcgc acgagcggtg actgccccgg gatgtagacc gcaccgagca gcgtgccgta
     5881 cgaattctcg gtcgtccacg atggcttgcc ttctgcggcg atgagcccct ccgcggtgcg
     5941 acgcacgcgc cacgtcttgt ccttataggt aacgacgagc ggcgggtcgt ctgcagatgc
     6001 gggcttggcg gcgccgctca atagatcgac cgcgacggct ttctcgccgc gtcgccagat
     6061 cacgttgtcg ccatcacgaa agacggcgag ctcgccatcg cttgcggcaa actcggcgac
     6121 gagctcgcgc ttcgcatgga tcgcgacgta tgcctgatcc tggcccgtcg cggtcacgac
     6181 gcgcgcacag ccgagcagcg tgtcttcgtc gggaacgttc ggcgctgtca cgcagtcgcc
     6241 gatcaagacg acgcggtcat cgatcacgat cggcggtgcg acgtgcgagc ccgcggcttt
     6301 cgtccacgcg atctggcgcg tcttgaaatc gacagcgatg aagccgaact gggagctcga
     6361 cacgatcgcg aggtcgccga cgaggaccgg gccaccgaca gtaaaccgcg gcgtgctcgt
     6421 cttcaccggc agcgtgatca cgcgaactgc ctcggtcagc ggaagcttcg cgagctcggg
     6481 ccacacgccg gcatctgcat ttgcgctcgc agcatcgcgc ggagctgctg cagccgcgtc
     6541 gtcgaagcga tgcgctgctt tccccttgca tgcggcaaga gctgcgacga acaggagccg
     6601 tttcacttcc ggccgagaag tctacgcaga aacccggagc gcttgggtgc ggcaggcacg
     6661 gtgtcggcgc gcgtcgattc gcgatctcgc ggctcagcac cctcgcgcgg tgcctcgacg
     6721 atcggatcgc cgggaccgcg cacgaagccc gcgacttcgc cgcgcgagac gagcagcagc
     6781 gccgccgaaa tcgcgcgact ccctgcatcg gcgtgacgcg agcgaacgat cgaagcaaag
     6841 cgcgcgaggc tcggtgcatc gtcgacggcc gccgtgacga gcaagcagcc gcgagccggc
     6901 gtcgctgcga tcagcagctc ggcgtcgagc agcggatgca gcgtcgacat gaatgcgcgg
     6961 tcgagcagct tctccgctgc atagaagctg ccggtgacga ccgcgagcgc gacgccaccg
     7021 acctcgagct cgtcgacctc gacttcttca tcggcgagat tgtcgagcgc gtcggcaaac
     7081 gcgaggtctg cgggcttcgg cagcgcttcg cgccggagca tgccgaacgt gttctcgcca
     7141 tcgacgccgc atacgacgac gggcagatcg agcggcgatg catcatcgag cagcggtcgc
     7201 caggtcacct cgtcgagctc gatgtcgctg cggtggcgca ggctcggcat gaggcggctc
     7261 gtcgatgcat cggcgttgcg atagaccgtt tcctccgcgg cgagcagaag cttgaacaag
     7321 ctctcgtcgg ggccgtcctc gatcacccgc tgcgcacggt tcgtcgggaa catcacggcg
     7381 gtgccgctgc cgacctgaaa gccaaacggc acgaccgcgc gatccgtctg gacccaccgg
     7441 cccggatact tcgcacgcag aagctcgccg gcgagcgcag cgagctcgag cacgcgacgc
     7501 caatactcgg gctcgtcgtc gcgatccggc ggcgcagcaa acgtctgctg ctcgagcatg
     7561 cgcaacgcga tcgctgcatc gcggctaccg acgcgcagct tcacgcgcga cgcgagctcc
     7621 ataaagcagc ggtccgcgag atcgccaacg gcttgctcgt catgcgtccc gagcgcttcg
     7681 agaatgcgcg cccgctcgag cacgccaccg cacgccgcca acgcttctcg cagaaacagc
     7741 ggcggctcgt ccagcagcga gtagtgcacg gccgcagtgg agtgtccttc ggccgccaga
     7801 tgcgagcgcg cattgagcac gcgagacatc gcgatcagcg aggacagctc gggttctgct
     7861 tcgatctcgg cacgacgccg atgatcggtc tgcacggcga tcggtcgtcc ctcgacccgt
     7921 gccgcgagat cgattttatc gatcgagcag tacacgtatc ggagatagca cggtcgctag
     7981 ccttcgtcgg caggcttctt cgctgcttct tcgccgttct tcttgcgctt ggcctgcgct
     8041 tcgcggaacc gatcagccca cccctcgacg ttcacctgtt tcacgcgagt tagcgcgagg
     8101 gcgccacgtg gcacgtcctt tgtcaccgtc gatccggagc caacatacgc accgcgacct
     8161 actgtgacgg gagcaacaag ctggctatcc gagccaatga aggcgccctc ttcgatcgtc
     8221 gtcttgtgct tgctgacgcc gtcgtagttg cacgtgatcg tgccagcgcc gatgttcgcc
     8281 ttcgcaccga ccgacgcatc gccgagatac gcgagatggt tcgccttcga gccggccatc
     8341 aggtgcgtct tcttcgactc gacgaagttg ccgatgtgca cgtcgtcatc gagacgcgag
     8401 cccggcctgc aatgcgtgaa cggtccgacc tggacgcgct cgccgatggt cgactcgctc
     8461 aacaccgagt acggcttgat gttcgtgtca tccgcgaccg tcacatcggt caggacgctg
     8521 cccagatcga tgcggacgcc gtcgccgatc ttcgtcttcc cacgcagcgc gacgttcggc
     8581 ccgatccaga cgtccttacc gagcggcccg acctccgcgt cgatgtagac gctgcgcgga
     8641 tcgaccatcg tcacgccggc acgcatccaa tgctcgttga tgcggcgtcg cgcctcggcg
     8701 tccaccgtcg acagatccac gcgatcgttg atgccactca cctcggtgaa cggcgcatcg
     8761 atcgacgtcg caccaccgcg cttgtacgcc gcggcaacga ggtccgtcag atagagctcg
     8821 cccttcgcat tattggcgcg cagatcctgg aggtcgcggc gcagatgtcc gagccgaatg
     8881 gcgtagaagc ccgcgttcgt atctttgatc tgccgctgct ccggcgtggc atcgggatgc
     8941 tcgacgatct tctcgagcac gcccttcgca tcgcgaacga gccggccgta cggcatgtcg
     9001 cgatcaggtc gcgtcgagag cagcgccatc ccgcccggcg cttctgcgca cgcacgcacg
     9061 agctcgctaa tacgctcgct ctgcaacagc ggcgcgtcgc cactgaggat gacgacaatg
     9121 cggtcctcgc cctcgttctg cacggtcgac aacgcgcact gcacggcgtg tccggtcccg
     9181 cgctgctccg gctgcagcgc gacgtcgacg ctgccaactc cgaagcttcc atcgagcgac
     9241 gccttcacg
//
LOCUS       DATLBZ010000674         3190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7978053, whole genome shotgun sequence.
ACCESSION   DATLBZ010000674 DATLBZ010000000
VERSION     DATLBZ010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7978053"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1843)
                     /locus_tag="VMZ53_33165"
     CDS             complement(107..1843)
                     /locus_tag="VMZ53_33165"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUS33416.1"
                     /translation="MGTEPLTQEAGTAFAVHDSAHAFLVIHVEGEERGSRVIDLPDGI
                     DVTFGRSRGATFNVESEKVSRMHARVKRMGETIEVEDLGSRNGTRVNGEKIDGIVKVT
                     AGDEIAIGSILAVVGATSGLRRASPIADDVRGESRLVAEVDRSLRYHRPLTLGLIRCT
                     NDAVVDAMARSLRPMDLMAEDAGDDYLVILPELTRTEGTAALERLLDFARGAGVIAKS
                     AQAQCPDDGTTVETLIGAVRASMRTGRPSQDVVSAPRDGTNEPIILDQAMKRVYTLVE
                     RIADTPMTVLILGETGVGKEGVAEAIHKKSSRRDKPLIKLNCAALPETLLESELFGYE
                     RGAFTGADKRKVGFFEAADGGTLFLDEIGEMPLPLQAKLLRVLERKVITRVGGTTEIA
                     TNARVVAATHRDLEGEVRLGRFRQDLMFRIGGFTIAIPPLRDRLAEILPLADHFARSA
                     AAETGRPAPSITPDARDALSAYSWPGNVRELKNAIERALVLCGDQITSADLPEKLRDA
                     GQRARPVGGGSAADMRGHLAEVERAAIVTALEAEDQNQTRAARRLGLSRRALIYKMEK
                     YGLKPPPSRADS"
     gene            1955..2611
                     /locus_tag="VMZ53_33170"
     CDS             1955..2611
                     /locus_tag="VMZ53_33170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33417.1"
                     /translation="MQGLIRCAVVAGLFCACHSDDTTTPDGDVGGDAGLTVRWTSRPV
                     TWPGDLGSTVTIKSARFHLDSLRVIGDAGPGDLRTSKDAFEVRWEEGRDPPADIQFDN
                     APTGLYSQIALQIDGNVVEDSYELRGTVVVNNNLEDFVIEDADPLAINLSIDKSLSAG
                     SKATIDIEIDFAHALSAVDFSAMNKSDGKLQLDESDAGITELRKKLLESFKTPNIAPR
                     "
     gene            complement(2608..>3190)
                     /locus_tag="VMZ53_33175"
     CDS             complement(2608..>3190)
                     /locus_tag="VMZ53_33175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33418.1"
                     /translation="LRTAESLFQTTANQTDADLSPPITLGMKVLENYVHAWLGPRMAG
                     LQRDPATLFDYVDRVIGGSWNGYARWIEPKWKDPAEVGTAKVDIPLRSIPNAVRELQD
                     RKRKRLDSPLSVTEWARMMVLFAVDHPSSGFRNLFKLGGKSGERTVQLAHRLHTLAAV
                     RNLVTHRASAGAATLSAFRRGYYTAFEDLVALA"
BASE COUNT          505 a          957 c         1161 g          567 t
ORIGIN      
        1 gcccacgcgg gcacatcgca acgaacgacc gcacgccgaa caccgtgccc acgtgggcac
       61 atcgcaccac tgaaacgcgc cgaatgtcgc agggacgtgc gacagcctag ctgtcagccc
      121 gagacggcgg cggcttcagc ccgtacttct ccatcttgta gatcagcgca cggcgcgata
      181 ggccgaggcg gcgtgcagcg cgcgtctgat tttgatcctc ggcttcgagc gccgtcacga
      241 tcgccgcccg ttcgacttcc gccaggtggc cgcgcatgtc ggctgcggag ccgccaccga
      301 cgggacgcgc gcgttgtcct gcatcgcgca gcttctcggg gaggtcggct gacgtgatct
      361 gatcgccgca gaggacgagg gcgcgctcga tcgcgttctt tagctcgcgt acgttgcccg
      421 gccagctgta ggcggagagg gcgtcgcgcg cgtcaggcgt gatcgatggg gcggggcggc
      481 ccgtttcggc ggcggcggag cgcgcgaagt gatcggcgag cgggaggatt tcggcgaggc
      541 gatcgcggag tggcgggatg gcgatcgtga agccgccgat gcggaacatg agatcttggc
      601 ggaagcggcc gagacgaact tcgccttcga gatcgcggtg cgtcgcggcg acgacgcggg
      661 cgttggtcgc gatttcggtg gtgccgccca cgcgcgtgat cactttgcgt tcgaggacgc
      721 ggaggagctt ggcttggaga gggagcggca tctcgccgat ctcgtcgagg aagagggtgc
      781 cgccgtcggc tgcttcgaag aagccgactt tccgtttgtc ggcgcccgtg aacgcgccgc
      841 gctcgtagcc gaagagctcg ctctcgagga gagtttcggg gagggcggcg cagttgagct
      901 tgatgagggg tttgtcgcgg cggctggatt tcttgtggat ggcttctgcg acgccttctt
      961 tgccgacgcc ggtttcgccc aggatgagga cggtcatcgg cgtgtcggcg atgcgctcga
     1021 cgagcgtgta gacgcgcttc atcgcctgat cgaggatgat cggctcgttg gtgccatcgc
     1081 gcggcgcgct gacgacgtct tgcgatgggc gaccggtgcg catgctcgcg cggacggcgc
     1141 cgatgagggt ttcgaccgtg gtgccgtcgt cgggacactg cgcttgggcg gatttggcga
     1201 tgacgccggc gccgcgcgcg aagtcgagga ggcgttcgag ggcggcggtg ccttcggtgc
     1261 gtgtgagctc ggggaggatg acgaggtagt cgtcgcccgc gtcttcggcc atgagatcca
     1321 tcgggcgaag ggagcgcgcc atcgcgtcga cgactgcgtc gttcgtgcag cggatgaggc
     1381 cgagcgtgag agggcggtga tagcggaggg agcgatcgac ttcggcgacg aggcgcgact
     1441 cgccgcggac gtcatcggca atcgggctgg cgcggcgcag gccgctggtg gcgccgacga
     1501 ccgcgaggat cgagccgatc gcgatttcgt cgcccgcggt gactttgacg atgccgtcga
     1561 tcttttcgcc gttgacccgc gtgccgttgc gcgagccgag gtcttcgact tcgatggttt
     1621 cgcccatgcg ttttacgcgg gcgtgcatgc gcgagacttt ctcgctctcg acgttgaacg
     1681 ttgcgccgcg cgagcgaccg aacgtgacat cgatcccatc gggcagatcg atcacgcggg
     1741 agccgcgctc ttcaccttcg acgtggatca cgaggaacgc gtgtgcgctg tcgtgcaccg
     1801 caaacgcagt tcctgcttct tgagtaagag gctctgtccc catcggtttg tacaaatcgt
     1861 gcacactggg caagacacgt gcacctgcgc gagcgcgaat tcgctcgatt aggttagttt
     1921 cgccgtggcc cttgcgttgc taccattagc agccatgcag gggttgatcc ggtgcgcagt
     1981 tgtcgccggc ttgttctgcg cctgccattc agacgacacc accacacccg acggcgacgt
     2041 cggcggtgat gcgggactga cggtgcggtg gaccagccgc ccggtgacgt ggcccggcga
     2101 cctcggctcg accgtcacga tcaagagcgc gcgctttcac ctcgacagcc ttcgcgtgat
     2161 cggcgatgca ggtcccggag atcttcggac gtcgaaggac gcgttcgagg tgcgctggga
     2221 agaaggccgc gatccgccgg cggacatcca attcgacaac gcgccgacgg gcctctactc
     2281 gcagatcgcg ctgcagatcg acggcaacgt cgtcgaagac agctacgagc tgcgcggcac
     2341 ggtggtcgtc aacaacaacc tcgaggactt cgtgatcgag gatgccgacc cgctggcgat
     2401 caacctgtcg atcgacaagt cgctgtcggc gggctcgaag gcgacgatcg atatcgagat
     2461 cgatttcgcg catgcgctct cggctgtcga tttctcggcg atgaacaaga gcgacggcaa
     2521 gctgcagctc gacgagagcg atgcaggcat caccgagctg cgcaagaagc tgctcgagag
     2581 cttcaagacg ccgaacatcg cgccgcgcta ggcgagggcg acgaggtctt cgaacgcggt
     2641 gtagtagccg cgacggaatg ccgagagcgt ggcggcgccg gcggacgcgc ggtgcgtgac
     2701 gaggttgcga accgcggcga gcgtgtggag gcgatgcgcg agctgcacgg tgcgttcgcc
     2761 ggacttgccg ccgagcttga agaggttgcg gaagcccgac gacggatggt cgacggcaaa
     2821 gaggaccatc atgcgcgccc actcggtcac cgacagcggt gagtcgaggc gcttgcgctt
     2881 gcgatcttgc agctcgcgga cggcgttggg gatcgagcgc agcgggatgt cgacctttgc
     2941 ggtgccgacc tccgcggggt ctttccactt cggctcgatc cagcgcgcgt agccgttcca
     3001 gctgccgccg atcacgcggt cgacgtagtc gaacagtgtt gcggggtcgc gctgcaagcc
     3061 ggccatgcgc gggccgagcc acgcgtgcac gtagttctcg agcaccttca tcccgagtgt
     3121 gatcggcggc gagaggtcag cgtctgtctg gttcgcggtc gtttggaaca gcgactcggc
     3181 ggtgcgcagg
//
LOCUS       DATLBZ010000675        10161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10222667, whole genome shotgun sequence.
ACCESSION   DATLBZ010000675 DATLBZ010000000
VERSION     DATLBZ010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10222667"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..79)
                     /locus_tag="VMZ53_33180"
     tRNA            complement(5..79)
                     /locus_tag="VMZ53_33180"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Asn,seq:gtt)
     gene            complement(248..1192)
                     /locus_tag="VMZ53_33185"
     CDS             complement(248..1192)
                     /locus_tag="VMZ53_33185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33419.1"
                     /translation="MNLKKLLQVSTGLLFIAGAASTAFADDEAGASADANAEATVSTD
                     PNAAGGAGAGMDANATASVDAGAGLSMLWPQSVIDRPYVVPAGTIGVGASLGVAKASF
                     TFVDPVTGMSSTTSATGEGMGITAAYGISDALNAGVSYGFSLHEFEIKGPLTVYGAFK
                     LAHSAKMSVAASASFTYDVGAETKGINAGLGLRYNVAPKIAVFTGAPFGPGPVGEHLS
                     LSLDSDGPISFDVFAGAGLQATPQAFVYLSTNVAHINISNDATGIFGADFVPIQLGGQ
                     FSVNKNIDAQAAFILPDLKNAKFDLFAFTVGATYYVGK"
     gene            complement(1286..2443)
                     /locus_tag="VMZ53_33190"
     CDS             complement(1286..2443)
                     /locus_tag="VMZ53_33190"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS33420.1"
                     /translation="MSRGRVLILWNQLDDDVVELWRRDGRRAPDWDPNLLVEPWDTVA
                     EEIEQIVQCVKDGGYDAKSINIRDNFETLLETLRDERPDVVLNLVEWFHDDLEHETHV
                     PAIYELLAQSYTGNRPLALSLCQKKPQAKALLAAAGLPVPRGIVVDLATGRAPDKLDL
                     RYPLIVKPAFDDASGGIDAGSVVRDRASLDARVQMVVGDHKMPALVEEFIEGREIHCA
                     ILGNSPPSPLPLYEMQFKKGAIDNEGRALPNIITYRAKWDPYSRDYYGMESKCPAPNL
                     SDELAARMQAIAVRAYQALGCRDYARVDMRLAADTNEPYILEVNPNPDLADGCAFAQC
                     VRASGRTYAQAIQEIVGYALERSKTKIKAEPFPSEQLVIEYKAQRGGRTTR"
     gene            complement(2507..4333)
                     /locus_tag="VMZ53_33195"
     CDS             complement(2507..4333)
                     /locus_tag="VMZ53_33195"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33421.1"
                     /translation="MAVRQPGDDDRATVDLGPRPAMVDGGEGERPPTDPGDLGPDEGA
                     TLADRSKIEATATLTPPSLPGPAPDVHALPTQQLPRKNADSHATRYSTASSPVEAMRL
                     EELARTQIFLKVAMITCLAGVGIAFLTGGDPIAFRVVMAGCMVTMLGSLWMFMKVRDP
                     ATFAHRKVIAPALVIAFGAFGGVYYWGPASPVAAMITYGIYFFSLGANARITFAMYIL
                     VSILHGALLVLIATGIVIDRGIVNIQHLRWIDQLGVLAVIEFLYFITFYTARTSQRVT
                     LEHVMKLEQAVRGVAQREALLAEARAELDRALKVGGPGRYTDQVIGGFKLGLLIGRGG
                     MGEVYEAQGPQGEAAVKLLHVGTLNDPTHVARFVREAQTVSKLDTPHVVRVLEVGTTA
                     GEVPYLAMERLRGHDLAHQLRKQRRLQLPDARMLALHVAQGLEAARVAGIVHRDLKPH
                     NVFLGQDSRWKILDFGVSKQGGSGTLTKGHVIGTPAYMAPEQARGENVDHRADVYSLA
                     AILYRSVTGHPAFTGKDVPTTLYDVVYRVPTQPSLLVQLPADIDRVLALGLAKDVRDR
                     FANALELADWFAAAVTDELTPEQRRRADDLLARHPWGTRQGS"
     gene            complement(4378..5244)
                     /locus_tag="VMZ53_33200"
     CDS             complement(4378..5244)
                     /locus_tag="VMZ53_33200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33422.1"
                     /translation="MHKSWAVLVLIILGGCDLYFGGDDEPPPCDYGGIGVDGYPSQSL
                     RDPYSGICQDFGYPYPCDDVCGPCAYGGAEKSDIANQPDWGACYSACEGLGENACIQT
                     AGCFAAYLDDPAADGKRDFYACWQTPPSGPIHGTCTGNDAQQCSRHDDCSAVYADSFS
                     SLQFLTCVPESSHTTCAGSDCAPGYHCEEHCYGDPSTNTMDVCQAYCVTDLTCAAVDC
                     APGYECAEVCDQGSTCHPTCVPTTACETLATETACQNRSDCTPVYIGMNCTCTIYGCS
                     CQDLTYDRCETL"
     gene            5364..5945
                     /locus_tag="VMZ53_33205"
     CDS             5364..5945
                     /locus_tag="VMZ53_33205"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HUS33423.1"
                     /translation="MHPLLAFLCFFRILFGRRLPAEAVQFLPEGTEPKQLPEKVEAKP
                     EVKPEPKAEPKPEKPKVPTSQHHRDGALALLALLQREGRFVDFVRDSVDGASDADIGA
                     AAREVHRGCRKVIDQHLSIEPVMPGAEEAKVSVPKGFDPAEVRLIGEAKGEPPYRGTL
                     RHHGWRVVDAKLPTLAEGVDRAVIAPAEVELGS"
     gene            5945..7702
                     /locus_tag="VMZ53_33210"
     CDS             5945..7702
                     /locus_tag="VMZ53_33210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUS33424.1"
                     /translation="MRILGIDLGTTNSALAVAGETEAAHALGISQVVGPGEIAERPTL
                     PSFLLLPSENEVPPAQLQLPWSGPMKYAVGTFARERGAELPHRLVSSAKSWLCNPSID
                     RTAQVLPFRGAQRELDEAMQGGERVSPVNASARYLAHLRAAWDDANPDEPADEQEILV
                     TVPASFDPVARELTVVAAREAGFEKITLLEEPQAAFYAYLASRGDAWRKDLKPGDIVL
                     VCDIGGGTTDFSLIEVADDGGSLSLERIAVGDHILLGGDNMDLALSAIVQRDLGAGLD
                     ALQQRALVHACRRAKEQLLSEDAPKSVPVAILSRGSKLIGGTKKADIKRETAEQLLVD
                     GFFPVVGSDAKAERRRSVGLREMGLPYAHDPAITRHLAEFIGRFGKMPTAVLFNGGVM
                     KADKLRTRIVELLRTWAGRDVKVLQGADLDLAVALGAAHYGAVRRGKGVRIRGGTARA
                     YYIGIESAMPAIPGFAPPVKALCVAPQGLEEGSTVELPDDELGLLVGETSTFRFFAAA
                     TRKEDSAGALVDADAEGVVELDPVEKLVEAEGDRKSGDMVPVRLEAHVTEVGTLELWC
                     VARDGHGRWKLEYSVRERD"
     gene            complement(7699..8862)
                     /locus_tag="VMZ53_33215"
     CDS             complement(7699..8862)
                     /locus_tag="VMZ53_33215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33425.1"
                     /translation="MGETCSVCGVPVAGANILYTPDGRIVCPACFAKAPPVGDLAKPP
                     WKMFAIAGAVAGVIPFGVNMSTSSSTMVNGEVTSFVYRDWIAIACGVVAIMLGIIATA
                     AARKEQLQRGLAFAAGIGVVLLGGAQIARGFGVFADPRGGGSGSYTSTSITTSPSMVH
                     VEDDTPKGDPQSPETCPDEESCFQLGRELNKAKDTANAVKAYERACKKGAMGGCFNGG
                     LIYINEDPPDVVKAMPMFQKACDQNDLQACTNLGRIYATDGPTKNPAEAIRTLNVACV
                     AGSPVGCHYYGVVLDQAIDGNEAPDKARMAFEMGCKGNEKDPGVGDSCFYLGEMKRRG
                     IGGDKDAKGALAAFTDGCKLGNQDACKALEAPAEEPAKAAKPTKKTATKKKRR"
     gene            8892..9365
                     /locus_tag="VMZ53_33220"
     CDS             8892..9365
                     /locus_tag="VMZ53_33220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33426.1"
                     /translation="MASGLDTIAHVVLPNLMKIKGASTLVGAMERRDVSAFAPVWEQT
                     GVEHEPQCISKEKDPWRYGVLSLPTPTEMGEAYMCAFMAKKNDASVTAYFTLVYDYVL
                     KTKTARTMICEVERGSTTKIGEGPPITGDFQTDASAFVDAVNQIVTRRSAPKLYT"
     gene            complement(9362..>10161)
                     /locus_tag="VMZ53_33225"
     CDS             complement(9362..>10161)
                     /locus_tag="VMZ53_33225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33427.1"
                     /translation="RHCETPTKEYEGPIVEFAEGEIRLRGTHAGYRSGIEISANAGGV
                     IEAEYVADHPVGHYRYRKGSIVIAEGAFDSEGRLDGEWIFRMPGSGQELTRTTFTHGT
                     GSIEIWDWRGSQPKVTARLQCKDGLLDGAQMFHLLSAPATYDHRVDGEYRRGLPDGAW
                     KLTREKDNVILYSGAYSAGTPTGTWHVIAEHPCIAQMRGGDTVDCIYGKDLPYTCTDR
                     GGCKFDSKPVIPHSGADIEPVDDDALIAPRAVRNAKRCELGEPYLSS"
BASE COUNT         1703 a         3241 c         3429 g         1788 t
ORIGIN      
        1 tggttggctc cgaaggaggg tttcgaaccc ccgacccggc ggttaacagc cgcctgctct
       61 accactgagc tacttcggaa gaagggaccg aattaatgac gtatctgggg cgggggtgtc
      121 aaggcaccgc gcgcgggcgg cggcccgaac ggccgtgaaa ttcagcggat ttgcgcgaaa
      181 aacgaaaggg actgacgccg gcacggagcc gaacgtcaat ccctgagagc ccgcgagcta
      241 gaggggctta cttgccgacg tagtacgtcg cgccgaccgt gaaggcgaag aggtcgaact
      301 tcgcgttctt gagatccggg aggatgaacg cggcttgggc gtcgatgttc ttgttgaccg
      361 agaactgacc gccgagctga atcggcacga agtccgcgcc gaagatgccg gtcgcatcat
      421 tcgagatgtt gatgtgcgcg acgttcgtgg agaggtagac gaacgcttgc ggggtggctt
      481 ggagaccagc accggcgaag acgtcaaacg agatcggacc gtcggagtcg agcgagagcg
      541 agaggtgctc gcccaccggg ccggggccga acggcgcgcc cgtgaagacc gcgatcttgg
      601 gggctacgtt gtagcggagg ccgagacctg cgttgatgcc tttggtttcg gcgccgacgt
      661 cgtaggtgaa gctggcgctt gccgcgacgg acatcttggc cgagtgcgcg agcttgaacg
      721 cgccgtagac cgtgagcggg cctttgatct cgaactcgtg cagcgagaaa ccatacgaga
      781 cgccggcgtt caacgcgtcg ctgatgccgt acgcggcggt gatgcccatg ccttcgcccg
      841 tcgcgctcgt cgtcgagctc atgcccgtga cggggtcgac gaacgtgaac gacgccttcg
      901 cgacgccgag gctcgcgccg acaccgatcg tgcctgctgg gacgacgtac ggacgatcga
      961 tgaccgactg tggccagagc atcgagaggc ctgcgcctgc gtcgacggac gccgtggcgt
     1021 tggcatccat tcctgcgcct gcaccaccgg ccgcattcgg atcggtagag acggtggctt
     1081 cggcgttggc gtcagcgctc gcacccgctt cgtcgtcggc gaaggcggtg gaggctgcgc
     1141 cggcgataaa caacaggccg gtgcttactt gcaaaagctt cttcaaattc atcacgagct
     1201 cctttagcgg atgcgcgtaa tgatcgaacg tcagcgtgag ctactgcaag ttttcgcggc
     1261 agatcactta cggaagtgat ctcggctagc gagtagtgcg tccgccgcgt tgcgctttgt
     1321 actcgattac gagctgctcg gacgggaacg gctcggcctt gatcttcgtc ttggagcgct
     1381 cgagcgcgta gcccacgatc tcttggatcg cttgggcgta cgtgcggccg gaagcgcgca
     1441 cgcattgcgc gaaggcgcag ccgtcggcga ggtccgggtt cggattgacc tcgaggatgt
     1501 acggttcgtt cgtgtcggcg gcgaggcgca tgtcgacgcg cgcgtagtcg cggcagccga
     1561 gagcttgata ggcgcggacc gcgatcgctt gcatgcgcgc ggcgagctcg tcgctgaggt
     1621 taggggccgg gcacttggat tccatgccgt agtagtcgcg gctgtaggga tcccattttg
     1681 cgcggtacgt gatgatgttc ggcagcgcgc gaccttcgtt gtcgatcgcg cctttcttga
     1741 actgcatctc gtagaggggc agcgggctcg gcggcgagtt gccgaggatc gcgcagtgga
     1801 tctcgcgacc ttcgatgaat tcttcgacga gcgcgggcat cttgtggtcg ccgaccacca
     1861 tttggacgcg tgcgtcgagc gacgcgcgat cgcggacgac cgagccagca tcgatgccgc
     1921 ccgaggcgtc gtcgaacgca ggcttgacga tcagcgggta gcgaaggtcg agcttgtcgg
     1981 gcgcgcgccc ggtcgcgagg tcgaccacga tgccgcgcgg aaccggaagg ccggccgcgg
     2041 cgaggagggc tttggcctgc ggcttcttct gacagagcga gagcgcgagt ggacggttgc
     2101 ccgtgtacga ctgggcgagc agctcgtaga tcgcggggac gtgcgtctcg tgctcgaggt
     2161 cgtcatgaaa ccattcgacg aggttcagga cgacgtccgg acgttcgtcg cgcagcgtct
     2221 cgagcagcgt ctcgaagttg tcgcggatgt tgatggactt cgcgtcgtag ccaccgtctt
     2281 tgacgcattg gacgatttgc tcgatctctt cggcaaccgt gtcccacggc tcgaccaaga
     2341 gattgggatc ccaatccggc gcgcggcggc catcgcgacg ccagagctcg acgacatcgt
     2401 catcgagctg gttccacaag atcagtacgc gtccacgcga catgcaaaca acgctgctac
     2461 tcaactcgta ccacgcaagt ccgcgtgagg gaatcgagat gagatctcag gagccttggc
     2521 gcgtgcccca cggatggcgg gcgagcaggt catcggcgcg gcggcgttgc tcgggcgtga
     2581 gctcgtctgt cacggcggcg gcgaaccagt cggcgagctc gagcgcattc gcgaagcggt
     2641 cgcgcacgtc tttcgcgaga ccgagcgcga ggacgcgatc gatgtcagcg ggcagctgca
     2701 cgaggagcga tggctgcgtc ggtacgcggt aaacgacgtc gtagagcgtc gtcggtacgt
     2761 ctttgcccgt gaacgcggga tggcccgtga ccgagcgata gaggatcgcg gcgagcgagt
     2821 agacgtccgc gcggtggtcg acattttcgc cacgcgcttg ctccggcgcc atgtaggcgg
     2881 gtgtgccgat cacgtggcct ttcgtcagcg tgcccgagcc gccctgcttg ctgacaccga
     2941 agtcgaggat cttccagcga ctgtcctggc cgaggaacac gttgtgcggc ttgagatcgc
     3001 gatggacgat gccggcgacg cgggccgctt cgagtccttg cgcgacgtgc agcgcgagca
     3061 tgcgggcgtc gggcagctgg agccggcgct gcttgcgaag ttggtgcgcg aggtcgtggc
     3121 cgcggagccg ttccatcgcg aggtagggga cctcgcccgc cgtcgtgccg acctcgagca
     3181 cgcgaacgac gtgcggggtg tcgagcttgg acacggtctg cgcttcgcgc acgaaccgcg
     3241 cgacgtgcgt cggatcgttg agcgtgccga cgtgcagcag cttgaccgcc gcctcgccct
     3301 gcggaccttg cgcctcgtag acctcgccca tcccaccgcg gccgatgagc aggccgagct
     3361 tgaagccgcc gatcacttgg tcggtgtagc ggccgggacc gccgaccttg agggcgcgat
     3421 cgagctcggc gcgggcttcg gcgaggagcg cttcgcgttg ggcgacgccg cgcaccgcct
     3481 gctcgagctt catgacgtgc tcgagcgtga cgcgctggct cgtgcgtgcc gtatagaacg
     3541 tgatgaagta gagaaactcg atcacggcga gcacgccgag ttggtcaatc cagcgcaggt
     3601 gttggatgtt gacgatgccg cgatcgatga cgattcccgt cgcgatcagc acgagcaatg
     3661 cgccgtggag gatcgacacc aggatgtaca tcgcgaacgt gatgcgggcg ttggcgccga
     3721 ggctgaagaa gtagatgccg tacgtgatca tcgcggcgac gggcgacgcc gggccccagt
     3781 agtagacgcc gccgaatgcg ccgaacgcga tcacgagcgc gggcgcgatg accttgcgat
     3841 gcgcgaaggt ggccgggtcg cgcaccttca tgaacatcca cagcgagccg agcatggtga
     3901 ccatgcagcc cgccatcacg acgcggaagg cgatcggatc gccgcccgtc agaaatgcga
     3961 tgccgacgcc ggcgaggcac gtgatcatcg cgaccttcag gaagatctgc gtgcgtgcga
     4021 gctcctcgag gcgcatcgct tcgaccggtg agctcgcggt cgagtagcgc gtcgcgtgcg
     4081 agtcggcgtt cttgcgcggc agctgctgcg tcggcagcgc gtggacgtcg ggcgccgggc
     4141 cgggcagcga gggcggcgtc agcgtcgcgg tcgcttcgat cttcgaccgg tcggccagcg
     4201 tcgcgccctc gtccgggcct aagtcacccg gatccgtcgg cggtcgctcg ccttcacccc
     4261 cgtcgaccat cgctgggcgc gggcccaaat cgacagtcgc gcggtcgtca tcccccggtt
     4321 gccggacggc cacagctcaa gcatatgccc ggcggaaggc ccaggccaac gttgtggcta
     4381 gagcgtctcg cagcggtcgt acgtcaggtc ctgacacgag cagccataga tggtgcacgt
     4441 gcagttcatg ccgatgtaca ccggcgtgca atcgctacga ttctggcagg ccgtttccgt
     4501 cgcgagggtc tcgcaggcgg tcgtcggcac gcacgtcggg tgacacgtgc tgccttggtc
     4561 acagacctca gcgcactcgt agcccggcgc gcagtcgacc gcggcgcagg tcaggtctgt
     4621 tacgcagtac gcctggcaga cgtccatcgt attcgtcgac ggatcgccgt agcagtgttc
     4681 ctcgcagtga tagcccggcg cgcagtcgct tccggcgcac gtcgtgtgcg aggactcggg
     4741 cacgcacgtc aggaactgca gcgacgagaa gctgtcggcg tagaccgcgc tgcagtcatc
     4801 gtgacgcgag cactgctgcg catcgttgcc cgtgcaggtg ccgtgaatcg ggcccgacgg
     4861 cggcgtctgc cagcacgcgt agaagtcgcg cttgccgtcg gccgccgggt cgtcgaggta
     4921 cgccgcgaag cagccggccg tctgaatgca ggcgttctcg cccaggcctt cgcatgcgga
     4981 gtagcaagcg ccccagtcgg gctgatttgc gatgtcgctc ttctctgcgc ccccgtatgc
     5041 gcatgggccg cagacgtcgt cgcacgggta tgggtaaccg aagtcttggc agatgcccga
     5101 atacgggtcg cgcagcgact gcgatggata cccatcgacg ccgatgccac cgtagtcgca
     5161 cgggggcggt tcgtcgtcgc caccgaagta gagatcacag ccgccaagga tgatgaggac
     5221 gagcacggcc cagctcttgt gcatatccgg ccgttcagca agcagcgcgc caccgcaagt
     5281 gcgcgagaca acgacacgaa acctcagttt ttcgacgcac gtgaggtggt ttttgagccc
     5341 tgccctcatt agaacagcag cgcatgcatc ccctgctcgc gttcctctgc ttctttagga
     5401 tcctgttcgg gcgcaggctc cctgcggagg ccgtgcagtt tttgccagaa ggcaccgagc
     5461 caaagcagct tcccgaaaag gtcgaggcga agcccgaggt gaaaccggag ccaaaggccg
     5521 agcccaagcc cgagaagccg aaggtgccga cgtcacaaca tcatcgtgac ggtgcgctcg
     5581 ctctgctcgc tctgctccag cgcgaaggcc ggttcgtcga tttcgtgcgc gactcggtcg
     5641 acggcgcgtc ggacgcggac atcggtgccg ctgcgcgtga ggtccatcgc ggttgccgca
     5701 aggtgatcga tcaacatcta tcgatcgagc ccgtgatgcc gggagcggaa gaagcgaagg
     5761 tctcggtgcc gaaaggtttc gaccccgctg aagtgcggct catcggtgag gcgaaaggcg
     5821 agccgcccta tcgaggcacg cttcgccacc acggctggcg cgtggtagac gccaagctgc
     5881 caacgctggc tgaaggcgtc gatcgcgccg tgattgcccc ggccgaagtg gagctcggaa
     5941 gctgatgcgc atcctcggaa tcgatctcgg tacgacgaac tcggcactcg cggtcgcagg
     6001 cgagaccgaa gcggcgcacg cgctcggcat ctcgcaggtc gtcggtcccg gcgaaatcgc
     6061 cgagcgcccg acactcccct cgttcctgct gctgccgagt gagaacgagg ttccgcccgc
     6121 gcagctgcag ctgccgtggt cgggcccgat gaagtacgcg gtcggcacgt tcgcacgcga
     6181 gcgcggcgcg gagctgcccc atcgtctcgt ctcgagcgcg aagtcgtggt tgtgcaatcc
     6241 gtcgatcgat cgcacggcgc aagtcctgcc gtttcgcggc gcgcagcgcg agctcgatga
     6301 agcgatgcag ggcggcgagc gcgtatcgcc ggtcaatgcg tcggcgcgct acctcgcgca
     6361 tctgcgtgcg gcatgggacg acgcgaatcc cgacgagccg gccgacgaac aagagattct
     6421 cgtcacggtg ccggcgtcgt tcgatcctgt tgcgcgcgag ctgaccgttg ttgccgcacg
     6481 cgaggcgggc ttcgagaaga tcacgctgct cgaagagccg caggccgcgt tctacgcgta
     6541 cctcgcctcg cgcggtgatg cgtggcgcaa ggatctaaaa cccggcgaca tcgtgctcgt
     6601 ctgcgatatc ggcggcggca cgacggactt ctcgctgatc gaagtcgccg atgacggcgg
     6661 cagcctctcg ctcgagcgca ttgccgtcgg cgatcacatt ctgctcggcg gcgacaacat
     6721 ggacctcgcg ctctccgcga tcgtgcaacg cgatctgggt gcaggcctcg atgccctgca
     6781 gcagcgcgcc cttgtccacg cgtgccgccg cgcgaaagag cagctgctct ccgaagacgc
     6841 gccgaagtcg gtccccgtcg cgatcctcag ccgcggcagc aagctgatcg gcggcacgaa
     6901 gaaagccgac atcaagcgcg agacggccga gcagctgctc gtcgacggct tcttccccgt
     6961 cgtcggttcg gatgccaagg cagagcgccg ccgctcggtc ggccttcgcg agatgggtct
     7021 gccgtacgcg catgatcctg cgatcacgcg ccacctcgca gagttcatcg gtcgcttcgg
     7081 caagatgccg acggccgtgc tcttcaacgg cggcgtgatg aaggccgaca agctgcgcac
     7141 gcgcatcgtc gagctgcttc gtacgtgggc cggtcgcgac gtcaaagtgc tgcaaggtgc
     7201 cgacctcgat ctcgcggtcg cgctcggcgc agctcactac ggcgcggttc gccgcggcaa
     7261 aggcgtgcgc atccgcggcg gcacggcgcg cgcgtattac atcgggatcg agagcgcgat
     7321 gccggcgatt ccgggattcg cgccgccggt caaagcgctg tgcgtcgcgc cgcagggcct
     7381 cgaagagggc tcgaccgtcg agcttcccga tgacgagctc ggcctgctcg tcggcgagac
     7441 atcgacgttc cgattcttcg ccgccgccac acgcaaggaa gattcggccg gcgcgctggt
     7501 cgatgcggat gccgaaggcg tggtcgagct cgacccggtc gagaagctcg tcgaggctga
     7561 aggcgatcgc aagtctggcg atatggtgcc ggtccgcctc gaagcgcacg tcaccgaagt
     7621 cgggacgctg gagctgtggt gcgtcgcgcg cgacggtcac ggacgttgga agctcgagta
     7681 ctcggtgcgc gagcgcgact agcggcgctt cttcttcgtc gcggtcttct tcgttggctt
     7741 cgcggccttc gccggctcct ccgcaggagc ttcgagcgcc ttgcacgcgt cctgattgcc
     7801 gagcttgcac ccgtcggtga acgccgcgag cgcgcccttc gcgtccttgt cgccaccgat
     7861 gccgcgcctc ttcatctcgc ccaggtagaa gcacgaatca ccgacgcctg gatccttctc
     7921 gttccccttg caccccatct cgaacgccat gcgtgctttg tccggagcct cgttgccatc
     7981 gatcgcttgg tcgagcacga ccccgtagta gtggcagcca accggggatc ctgccacgca
     8041 tgcgacgttc agcgtcctga tcgcttccgc agggttcttc gtcggcccgt cggtggcgta
     8101 gatgcggccg aggttcgtgc acgcttgcag atcattctgg tcgcacgctt tctggaacat
     8161 cggcatggcc ttcacgacat ccggtggatc ctcgttgatg tagatgagcc ccccgttgaa
     8221 gcagccgccc atcgcgccct tcttgcacgc cctctcgtac gccttgaccg cgtttgccgt
     8281 gtccttggcc ttgttgagct cgcgaccgag ctgaaagcag ctctcttcgt ccggacacgt
     8341 ctccggactc tgcggatcgc ccttcggcgt gtcgtcctcg acgtgcacca tggaaggcga
     8401 cgtcgtgatg ctcgtcgacg tatacgagcc cgagccgccg ccgcgcggat ccgcaaagac
     8461 gccgaagccg cgcgcaatct gcgcgccgcc gagcaacacc acaccaatgc ccgcagcaaa
     8521 cgcgagcccg cgctgcagct gctccttgcg cgctgccgcc gtcgcgatga tgccgagcat
     8581 gatggcgacg acgccgcacg cgatggcgat ccaatcgcga tagacgaacg aggtcacctc
     8641 gccgttcacc atcgtcgagc tcgatgtcga catgttcacg ccgaacggaa tgacgcctgc
     8701 gaccgcgcct gcgatcgcga acatcttcca gggcggcttc gccagatcac cgacgggggg
     8761 cgccttcgca aaacacgcag gacacacgat gcggccatcc ggcgtgtaca ggatgttcgc
     8821 accagcgaca ggcacaccgc acaccgagca cgtctccccc atcgcgccat tatcagcgct
     8881 attgttcgac catggccagt ggtctcgaca cgatcgcgca cgtcgttctc ccgaatctca
     8941 tgaagatcaa aggcgcgtcg acgctcgtcg gtgcaatgga gcgccgcgat gtctccgcgt
     9001 tcgcgccggt gtgggagcaa acgggcgtcg agcacgaacc gcagtgcatc tcgaaggaaa
     9061 aagacccgtg gcgctacggc gtgctttcgc tgccgacgcc gaccgagatg ggcgaggcat
     9121 atatgtgcgc gttcatggcg aagaagaacg acgcgtcggt caccgcgtac ttcacgctgg
     9181 tctacgacta cgtgctcaag acgaagactg cgcgcacgat gatctgcgag gtcgagcgtg
     9241 gaagcacgac gaagatcggc gagggcccgc cgatcaccgg cgattttcag accgatgcat
     9301 ccgcattcgt cgatgccgtg aatcagatcg tcacgcgccg cagcgcgccg aagctctaca
     9361 cctagctcga gaggtacggc tcgccgagct cgcagcgctt ggcattgcgc accgccctcg
     9421 gcgcgatgag cgcgtcatcg tcgacgggct cgatgtcggc gccgctgtgc gggatcaccg
     9481 gcttgctgtc gaatttgcag ccaccgcggt cggtgcatgt gtacggaagg tctttgccgt
     9541 agatgcagtc gacggtgtcg ccgccgcgca tctgcgcgat gcaaggatgc tccgcgatca
     9601 cgtgccatgt gccggtcggc gtaccggcgc tgtacgcgcc gctatagaga atcacgttgt
     9661 ccttctcgcg cgtgagcttc cacgcgccat ccggtagtcc gcgccggtac tcaccgtcga
     9721 cgcgatggtc gtacgtcgcg ggtgccgaga ggaggtggaa catctgcgcg ccgtcgagca
     9781 gcccgtcttt gcattgcagg cgcgcggtca ccttcggctg cgacccgcgc caatcccaga
     9841 tctcgatcga gcccgtgcca tgcgtgaacg tcgtgcgggt gagctcctgc ccgctgccgg
     9901 gcatgcgaaa gatccactcg ccgtcgaggc gaccctcgct gtcgaacgcc ccttcggcga
     9961 tgacgatcga gcctttgcga tagcggtagt gcccgaccgg gtgatcggcc acgtactccg
    10021 cttcgatgac accgccagcg ttggcggaga tctcgatgcc ggagcgatag ccagcgtgcg
    10081 tgccacgcag cctgatctcg ccctcggcga attcgacgat cgggccttcg tattctttgg
    10141 tcggcgtttc gcaatggcga t
//
LOCUS       DATLBZ010000676         5235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6492349, whole genome shotgun sequence.
ACCESSION   DATLBZ010000676 DATLBZ010000000
VERSION     DATLBZ010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6492349"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..762)
                     /locus_tag="VMZ53_33230"
     CDS             complement(85..762)
                     /locus_tag="VMZ53_33230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33428.1"
                     /translation="MCGEQSRTTIKWVFNRYPERGFQMDSCTDLGVSKVGVDVVDSAG
                     NVQSLQTDCGNGQLAFFGLAEGDYTVYVAPLDAGGNGLVTAPAQGTVTAGPVGDNREV
                     TVNVPVEAWIGPYDGSFLFRISWGGMSCETTDIKDQVVTLKVNGVVQSVLTDDGQMLN
                     GADKKPCKRLTDEFPQTAKHAAFGMATLLIEGYDATDNMRFSHQFDTFVGAGISNPTL
                     TFDVPTM"
     gene            complement(940..>5235)
                     /locus_tag="VMZ53_33235"
     CDS             complement(940..>5235)
                     /locus_tag="VMZ53_33235"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS33429.1"
                     /translation="LLDRVFGMFEPPRGTHYFLGAGVGPVTVDELMADHHIEVVNLAG
                     RGGDETAERSVTEWLETLRAACTKAQISLAQTRPDQDDVDGWVALLGEGGDEAAEAFD
                     ALSLIERRARAAKKWDTVAEVILGRIESAEAEQRAKELVSLSEVYETEIGDLARAFEA
                     VTTACQIAPDDDSIVARAEKLAAATGNWADLVSEASEIATEASDPRIASKWWARLGGW
                     YANKLDRLDYALPSLRRALELDSKNTNAYKALAETHRKSQKWADLAETLKAHAEVETD
                     QKTKVEALIDLGDLYETQLASSTKAAEAYQHAFDIDENNDDALAALERLYRRDEKWAN
                     LAKVLDRRADLAAEGGDKGRAAAIRTELATLRAEKLGDLEGAIARYEATVAANGSDVA
                     ALKALVDLYDKTGRTEDYLRTLERLGAVAPEGEKLATLRKLAAELEDRDATRARDAYE
                     KLLAADPNADDAYRGLERVLKNEGNWDELVAVHRRHIAAIKVPAARTELYVESAQIYE
                     RELEDPAKAIDALMNVLAIEDTNKTALVALPRLYQKTEQYDRAVEALTRHAATEGDKG
                     AALYAEAGRIAFEHLADPDAAQKHLEKAVALDAESFEALKTLGRLHGKRGNWEQAVEM
                     MLRADAAGPTRIERVALLWEAAQIADGKLQDPTRALELYERVLKLDPDHVEAGQRVAD
                     RLVAAKRWDDALPVLEMLARQAEGLDKLERSRREAQLGKAYEALHRTEKAARHFRLAV
                     DADPESLDAAIGLAAVLMAEAKVHEGSEQATEMWKEVDRRYRELLARHRTNISDAQTA
                     EIWYRLGAASRALGDDKKAEASFRRTLEKEPLHEPTLEAMVELGGARGEWRMVADAKR
                     AQIDALSKREGTDYLRAKLFEEIGDIYRERLKDVTSASDAFQEGIKIAPASRVLLHKL
                     LEAFTEQRQWRRAIESLDSLSGLEDSPERRARFHYTAAVIARDEMNDGELAIEKFGAA
                     LDDAPLTPKAFDGIEKLLIDRKDWKNLARAYRRQLKRLGTEAPTEKLLEMWTKLGDVY
                     AEHLNDTEAATEAYQVACELAPDDVARHEQLADLYLEAGESRRHEAIGELQFLLTHAP
                     DRVELYKALASLYRAEHELDKAFCVAQALVFLGAASSEERMLYEKFRPRQFTPAPRRL
                     TEELWQKAIIHPREDKAVGAIFASTLGALAAGTAQPITSFGLTPDNRTDLDRDPRPVS
                     KVVKYVAGVLAIDPAPMVWLQEQGDGLRVANTVGLGADRQKLVPSLLVGAPTIGKQDE
                     RELAFEVGKRMAYLRPERFVTLAVGTLPKLEAAFAAAVLASGVRTTDHEGKPYNPDNE
                     EAKKLAGQLQRQVPGPLLEQVGELSHKLSGRLGNGLIPNWRAATDLTANRVGFIVAND
                     FEVAAKGIATEGASLSAMSVKERLRDLLAYAVSEQYFQVRRHLGLHVRGEVSA"
BASE COUNT          803 a         1743 c         1737 g          952 t
ORIGIN      
        1 cgcctgcccc gcggctcaac gcagcggaac cgcagcgcgt caacgcgccc gacgcatggc
       61 cagccgagtt gccggcaaga ccgattacat cgtcgggacg tcgaacgtga gcgtcgggtt
      121 cgagataccc gcgccgacga acgtgtcgaa ctgatgcgag aagcgcatgt tgtcggtggc
      181 gtcgtagccc tcgatgagga gcgtcgccat accgaacgcc gcgtgctttg cggtctgcgg
      241 gaactcgtcc gtcagcctct tgcacggctt cttgtcggcg ccgttgagca tctgcccgtc
      301 gtcagtcaag acggactgaa cgacgccgtt gaccttgagc gtgacgacct gatccttgat
      361 gtccgtcgtc tcgcacgaca tgccgcccca cgagatgcgg aagaggaacg agccatcgta
      421 cgggccgatc cacgcttcga cggggacgtt gaccgtgact tcgcggttgt caccgacggg
      481 acctgccgtg accgtgcctt gcgcaggcgc ggtgacgagg ccgttgccgc ccgcatcgag
      541 cggcgccacg tacacggtgt aatcgccctc ggcgaggccg aagaacgcga gctggccgtt
      601 accgcagtca gtctgcagcg attgcacgtt gcccgcggaa tcgacgacat cgacgccgac
      661 cttcgagacg ccgaggtccg tgcacgagtc catctggaag ccgcgctcgg ggtagcggtt
      721 gaacacccac ttgatcgtcg tgcgcgattg ttcgccgcac acgctagtag cgcacgggtc
      781 gctgccaccg cctccacacg cagccaacaa cgaaactacg acgaagctgg taatacgcat
      841 cgcactcctc ccggcgagcg atgcttacca atgaaacagc ccgactggaa cgacaaactt
      901 catgtcgccc atcgggctgt ggcggcagag cgcggatccc taggccgaga cttcaccgcg
      961 gacgtgtagc ccgaggtgac ggcgaacctg gaagtactgc tcgctgacgg cgtacgcgag
     1021 gaggtcccgg aggcgctcct tcacggacat ggccgaaagg ctggcacctt ccgtggcgat
     1081 ccccttggcg gcgacttcga agtcgttggc gacgatgaag ccgacgcggt tggccgtgag
     1141 gtccgtggcc gcgcgccagt tcgggatgag gccgttgccg aggcggccgc tcagcttgtg
     1201 agaaagctca ccaacctgct cgagcagcgg gcccggaacc tggcgctgga gctggccggc
     1261 cagcttcttc gcctcttcgt tgtccgggtt gtacggcttg ccttcgtggt ccgtcgtacg
     1321 gacgccggag gcgagaaccg ccgcggcgaa cgcagcttcg agcttcggca gcgtgccgac
     1381 cgcgagggtc acgaagcgct cggggcgcag atacgccatg cgcttgccga cctcgaacgc
     1441 cagctcgcgc tcgtcttgct taccgatcgt cggcgcacca acgagcagcg acgggaccag
     1501 cttctggcga tcggcgccga ggcccaccgt gttcgcgacg cggaggccat cgccctgctc
     1561 ttgcagccac accatcggcg ccggatcgat cgcgagcacg ccagcgacgt acttcacgac
     1621 cttgctcacc ggacgcggat cgcggtcgag atccgtgcgg ttatcgggcg tgagaccgaa
     1681 cgacgtgatc ggctgcgcgg ttcccgccgc gagggcaccg agcgtcgacg cgaagatcgc
     1741 accgaccgcc ttgtcctcac gcggatggat gatcgccttc tgccacagct cctcggtcag
     1801 acggcgcggg gccggcgtga actgacgcgg gcggaacttc tcgtagagca tgcgctcttc
     1861 ggaagacgcc gcgccgagga acacgagtgc ctgcgcgacg cagaacgcct tgtccagctc
     1921 gtgctcggct cggtacaggc tcgcgagagc cttgtacagc tcgacgcgat ccggcgcgtg
     1981 cgtgagcaag aactgcagct cgccgatcgc ttcgtgccga cgtgactcgc cggcctcgag
     2041 gtagaggtcc gcgagctgct cgtgacgagc aacatcatcg ggcgcgagct cgcacgccac
     2101 ttggtacgcc tcggtggcgg cctcggtgtc gttcagatgc tccgcgtaga cgtcgccgag
     2161 cttcgtccac atctcgagca gtttctccgt cggcgcctcg gtaccgagac gcttcagctg
     2221 acggcgatac gcgcgcgcga ggttcttcca gtccttgcga tcgatcagca gcttctcgat
     2281 gccgtcgaat gccttcggcg tgagaggtgc gtcgtcgagc gccgcgccga acttctcgat
     2341 cgcgagctcg ccatcgttca tttcgtcgcg tgcgatcacg gcggcggtgt agtggaagcg
     2401 agcgcgacgc tccggcgagt cttcgagacc cgacagcgag tccagcgact cgatcgcacg
     2461 gcgccactgc cgctgctcgg tgaatgcttc gagcaacttg tggagcagca cgcgcgacgc
     2521 cggggcgatc ttgatgccct cttggaaggc atcgctcgca gacgtcacgt ccttgagacg
     2581 ctcgcgatag atgtcgccga tctcctcgaa cagcttcgcg cggagatagt cggtgccttc
     2641 gcgcttggag agtgcatcga tctgcgcgcg cttcgcgtcg gcgaccatgc gccactcgcc
     2701 gcgtgcacca ccgagctcga ccatcgcctc gagcgtcggc tcgtgcagtg gctccttctc
     2761 gagtgtgcgg cggaagctag cttctgcctt cttgtcgtcg ccgagtgcac gagacgcggc
     2821 accgaggcgg taccagatct cggcggtctg cgcgtcggag atgttcgtgc gatgacgcgc
     2881 gagcagctcg cgataccgac gatcgacttc cttccacatc tcggtcgcct gctcgctgcc
     2941 ctcgtggacc tttgcttcgg ccatgaggac ggcggcgaga ccgatcgcag cgtcgaggct
     3001 ctccggatcc gcgtcgacgg cgaggcggaa gtgacgggcg gccttctcgg tgcgatgcag
     3061 tgcttcgtac gccttgccga gctgcgcctc gcggcggctg cgctcgagct tgtcgagacc
     3121 ctcggcttga cgcgcgagca tctcgagcac cggcagtgca tcgtcccagc gctttgctgc
     3181 gacgagacga tcggcgacgc gctgacccgc ttcgacgtga tcgggatcga gcttgagcac
     3241 gcgctcgtag agctcgagtg cacgggtcgg gtcctggagc ttgccgtcgg cgatctgagc
     3301 cgcttcccag aggagagcaa cgcgctcgat ccgggtcggg cccgccgcgt ctgcacgcag
     3361 catcatctct accgcttgct cccagttgcc acgcttgccg tggagacggc cgagcgtctt
     3421 gagggcttcg aagctttctg cgtcgagcgc tacggccttc tcgaggtgct tctgcgcagc
     3481 gtcgggatcc gcgaggtgct cgaacgcgat gcgcccggct tcggcgtaga gcgctgcgcc
     3541 cttgtcccct tcggtcgcgg cgtgacgcgt gagcgcctcg acagcgcggt cgtattgctc
     3601 ggtcttttga tagagacgcg gcagcgcgac gagcgcggtc ttgttcgtgt cctcgatcgc
     3661 gagcacgttc atgagtgcgt cgatcgcctt cgccggatcc tcgagctcgc gctcgtagat
     3721 ctgcgcgctc tcgacgtaca gctcggtgcg agcggccgga accttgatcg ccgcgatgtg
     3781 acggcgatgc acggcgacga gctcgtccca gttgccttcg ttcttgagca cgcgctcgag
     3841 accgcgatac gcgtcgtccg cgttcggatc ggcggcgagc agcttctcgt aggcatcgcg
     3901 agcgcgcgtg gcgtcgcgat cttcgagctc ggcggcgagc ttgcgcagcg tcgccagctt
     3961 ctcgccttcg ggcgcgacgg cgccgaggcg ctccagcgtg cggaggtagt cctcggtgcg
     4021 gcccgtcttg tcgtagagat cgacgagcgc cttcagcgca gcgacgtcgc taccgttcgc
     4081 ggcaacagtc gcctcgtagc gcgcgatcgc gccttcgaga tcgccgagct tctcggcgcg
     4141 aagcgttgcg agctccgtgc ggatcgcggc agcgcggccc ttgtcgccac cttcggcggc
     4201 aaggtctgcg cggcgatcga ggaccttcgc gaggtttgcc cacttctcgt cgcggcgata
     4261 cagacgctcg agcgcagcaa gcgcgtcgtc gttgttctcg tcgatatcga atgcgtgctg
     4321 atacgcttcg gcggccttcg tcgacgacgc gagctgcgtc tcgtagaggt caccgagatc
     4381 gatgagcgct tcgaccttcg tcttctgatc ggtctcgact tcggcgtgcg ccttcagcgt
     4441 ctcggcgagg tcggcccact tctgcgactt gcgatgcgtc tcggcgagcg ccttgtacgc
     4501 gttcgtgttc ttcgagtcga gctcgagtgc gcgacgcagg gacggcagcg cgtagtcgag
     4561 gcgatcgagc ttgttcgcgt accagccacc gaggcgcgcc caccacttgg aggcgatgcg
     4621 cgggtccgac gcttccgtcg cgatctccga ggcctcgctc acgagatccg cccagttgcc
     4681 ggttgcggca gcgagcttct cggcgcgagc cacgatcgaa tcgtcgtcgg gcgcgatctg
     4741 acacgccgtc gtgacggcct cgaacgcacg cgcgagatca ccgatttcgg tctcgtagac
     4801 ttccgatagc gagacgagct ctttggcgcg ttgctcggct tctgcggact cgatgcggcc
     4861 gaggatgacc tcggcgaccg tgtcccactt cttcgctgcg cgcgcgcgac gctcgatgag
     4921 cgacaacgcg tcgaatgctt cggctgcttc gtcgccgccc tcaccgagga gcgcgaccca
     4981 gccgtcgacg tcgtcctgat cgggacgcgt ctgagcgagc gagatctgag ccttcgtgca
     5041 ggcagcacgc agcgtctcga gccattccgt caccgagcgc tcggcggtct catcgccacc
     5101 gcggcccgcg aggttcacga cctcgatgtg atggtcggcc atcaattcgt cgacggtcac
     5161 gggaccgacg ccggcaccga ggaagtagtg cgtgccgcgc ggcggctcga acatgccgaa
     5221 cacgcgatcg agcag
//
LOCUS       DATLBZ010000677         9244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7410923, whole genome shotgun sequence.
ACCESSION   DATLBZ010000677 DATLBZ010000000
VERSION     DATLBZ010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7410923"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VMZ53_33240"
     CDS             <1..706
                     /locus_tag="VMZ53_33240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33430.1"
                     /translation="VRSSPPAPRAQLAHTSSSSSSSTHSSKPSTMAALFQQPPAATQC
                     KDSKVETQVALVEAPAAESTGSTKKSNIGKSNAAKATGAKPASAKPQAIIATTKPSTA
                     IAKSTPIGKTTQPATSASTATTKPAAAIAKTTQSAPTTATAKPSVGAAKTTQPATTTA
                     TAKPSVGAAKTPQPATTTATTKPSTAAAKTTQPATTATTTKSATTKQQAVAKTTATTK
                     SATTKKPAASCGLDCI"
     gene            complement(988..2433)
                     /locus_tag="VMZ53_33245"
     CDS             complement(988..2433)
                     /locus_tag="VMZ53_33245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myxococcus cysteine-rich repeat containing
                     protein"
                     /protein_id="HUS33431.1"
                     /translation="MASARTVPALALLSLVAACAGDSNGQLEPDAGSSSSPDAAVPDP
                     RPDAPPAPVCGDGKRSPFEACDDGNTNSYDGCNAGCQVEVDCTCSGTPSSCTCTPTAT
                     TTTQTITTTPQRVETASLALDSTGQPHVSWFYSINFTDPVTNYSREHAHAMFAERSSP
                     TTWINNEIETWDQTQTTMSPSHFSLAFDGNALRAFFHRIYSTSGTFETATHTTTGSWT
                     FASANPTYSYDVVRAGGNWHALVGKTAFYDLHYQMGEPGAWTRDEALTGFSTSYPTRL
                     AVSGTGDVFLSSITPGTNHTSYNLKLSKRLNGFTWDTVYDVPTTGTCVYPIAHNPLVL
                     GNDLMTIEDGFSTGTSAQRWLRAHRLVNGTWTTETIADLSWYGYAPTCSAGGASWTTL
                     PMATAADANNQPHIVYAGPPWNNSTTIEDHYRDASGWHVRSFALAKATPLDMKIDAQG
                     TAHILATTPSSTAGSTRLVYLRINATGWTAL"
     gene            2495..3610
                     /locus_tag="VMZ53_33250"
     CDS             2495..3610
                     /locus_tag="VMZ53_33250"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HUS33432.1"
                     /translation="MRIAWLALVTAAACGGSGAGPDAAGDGSGSAPDAAVPTRYVAYV
                     SGGANIDWYDVDKASGVLTHVSSIAAFRTGANFLAVHGTRLYAVTSGDRVGAYTIDPA
                     TAGLTFINDVGTGGTGVAHVSVDRTGGYVMVANYGSGHIDVVPVRGDGGLGTPLAPIL
                     AGMKAHQIITDASNKFVLVPCLGDNKVAQYTFDASSGALTANATPKLDTASGAGPRHI
                     AIAPNAKHAYLINELNSTLSALAFDSATGRLTELQTVSTRESGASGSNTGAEIVVHPS
                     GKFVYASNRGDNNIAVFAINATTGMVTEVEHESTQGMTPRNFTIDPSGTLLYVANQNS
                     GTVVPLEIDPATGRLSPTASSVSVPTPQFIGIVELPL"
     gene            3623..3841
                     /locus_tag="VMZ53_33255"
     CDS             3623..3841
                     /locus_tag="VMZ53_33255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33433.1"
                     /translation="MDQVMCETCARMTREDALTIKTLEITETIHNACAPITHYAAKCR
                     TCGTKWLALEVYDEEGRQPSEWSWSVDK"
     gene            3858..5012
                     /locus_tag="VMZ53_33260"
     CDS             3858..5012
                     /locus_tag="VMZ53_33260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33434.1"
                     /translation="MRRLALLSLVAACAVPPAGAPSLASITPASGFTTEETAVRLQGV
                     FHLALSADLDHGTTDIEQCSASIGTTPLANVAWRSEQLIEATVPAGMPLGVYDVTVTC
                     GARVTTLPEAFTVLPEGLPTQPFGAPTLVAAIASPTSLEDDPSITADRLELFFNSDRP
                     GSLGGADIYVTTRATPSDAWTTPVAVPVVNTAYSETTPKISADGLTLYFSSDRPGGTN
                     ANYDVWVSTRPSRTGAWSAPTWVPELSSVASDSGANPTSSMLALVLCSDRDGAGDLYV
                     AKRAALGDPWGAPSALVEVNSAEGDADPTLAHGDRVIVFISARPGGLGGNDIYMATRS
                     SAAAPFDTPVLLPELNSAMQEEDPYISDDLRYILFTSTRSGDFEIYEASR"
     gene            complement(5009..5398)
                     /locus_tag="VMZ53_33265"
     CDS             complement(5009..5398)
                     /locus_tag="VMZ53_33265"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUS33435.1"
                     /translation="MQMFPLEVVESWHVAVNARDFEQLCAVTARDVELVGPRGSARGH
                     EMLRQWLGRAGFTAEPLRWFCGRGGQVVVEQRGRWHIPETTSERIVASAFRVSGGRVV
                     RYQRFEDLSEALATSSLTDDDEVLGRS"
     gene            5445..5894
                     /locus_tag="VMZ53_33270"
     CDS             5445..5894
                     /locus_tag="VMZ53_33270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33436.1"
                     /translation="MQLITERSGAMRPLYVHMHRLLLLLLVSGCLTTRVETMSWPTTV
                     HLRFSQVCPGSAVEVYDEHATYVPVAIVDEKVDVNVPAMRGGYDLRRGKKSRESDPAA
                     YPALRLRNGHEVVTVLSLHDVGAFPKDAEGRAVIPVRCSVAQPVPAT"
     gene            5923..6906
                     /locus_tag="VMZ53_33275"
     CDS             5923..6906
                     /locus_tag="VMZ53_33275"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HUS33437.1"
                     /translation="MSQPLFTFRQLPTLSEMLGDRGIVLADVMRDAGFTEMSTTEVTA
                     PLSKVQKLLELSGQKLESQALGLDLADRIPQGAYGVTEFVVRTSPTVRHALTAMCELA
                     PLINPALDMRYVADQLGCEVRFAYAGNRDALGETLNEYTVAYIAKQFAVLLQKALPLT
                     RAWFAHGRRQGGDEVSRRFGCSVGFQAADCGFAVAADVIAFTNPQGNEALYKFLLDQA
                     RTQLQNLGKHDVITQVTRVIEARISDADLSAATIAQALALSQRTLQRQLTDAGTSYRD
                     LLASIRRRRRAELERAGLADAEIAPRLGFASAKTMRRSLDDTGADEPAPEE"
     gene            6990..8720
                     /locus_tag="VMZ53_33280"
     CDS             6990..8720
                     /locus_tag="VMZ53_33280"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HUS33438.1"
                     /translation="MKRRTFLRWLAAAPSLPLIGSACTSGDPEEAMFPQGVASGDPSP
                     DGVLLWTRVESTSTERVLYEVASDRDFVTIVTTGEREVDDTTDHTLRVELTGLASGTT
                     YWYRFTVRGVTSRVGRTKTAPDPTADVPLRIALASCQDFGGRWFHAWRALVDRDDIDI
                     VVFIGDYIYETIGHLGVEWPADRTIELPDGLELDDEIKGSRAALTLADYRALYRQYRS
                     DPDLRDAHAHFPFVSLWDDHEFANDCWGDHATDFDDRHGDEASPDRRAAATRAYFEFQ
                     AIRRPFDGAAAFPNDLSIYRTLRWGKHAELVLLDERYYRDDDLVPEGPIDRDVGHIQM
                     NSAFGSRTFVIKDAFDAREAAASPTPTMLGTPQRDWAISAVTSSTATWKLLASPLVMA
                     QMAVDLTGYAQLPDQFRKRFYFKTDQWDGFRSERRALLEACSGVENLVVLSGDLHGFY
                     AAELYADFDAPGEPIAVEYATSAITAPTVDVQINGAIESNVFLEALGLKDLVPEFDGN
                     LLATNPHIKHADSTTNGLAIIEIGAAQVDVRFIRVSDVTSPTGRVIDEVAFQTPIGSR
                     RVVYSAPCSG"
     gene            8708..>9244
                     /locus_tag="VMZ53_33285"
     CDS             8708..>9244
                     /locus_tag="VMZ53_33285"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HUS33439.1"
                     /translation="MFRLNALLILGALAACGGAKTTTQPTTGQEPAGPVDPKEAAVNQ
                     VPASPVAGGKKAVVAPRLIAKPLAGDTTKTTIHRLSNGMTVYIAPDNKEPTITAYVAV
                     HAGGAYDPQNSTGLAHYLEHMLFKGSSKLGTQDYAKEKPHLDKIAALYDDLRKPGADA
                     DKILKEIDSETQAAAAFAI"
BASE COUNT         1633 a         3029 c         3084 g         1498 t
ORIGIN      
        1 cgtccgctca tcgccgcccg cgccgcgcgc gcagctcgcc cacacgtcgt cgtcgtcgtc
       61 gtcgtcgacg cactcgtcga agccgtcgac gatggccgcg ctgttccagc agccaccggc
      121 ggccacgcag tgcaaggact cgaaagtcga aacgcaggtc gcgctcgtcg aggcgcccgc
      181 cgccgagagc accggctcga cgaagaagtc gaacatcggc aagtcgaatg ccgcaaaggc
      241 gaccggcgcg aagccagcaa gcgcgaagcc ccaggcgatc atcgctacga cgaaaccctc
      301 gacggccatc gcaaagtcga cgcccatcgg aaagaccacg cagcccgcga cgagcgcgag
      361 caccgcaacg acgaaacccg cggcggccat cgcgaagacg acgcagtctg cgccgacaac
      421 cgcaacggcg aaaccctccg tgggcgccgc aaagacgacg caacctgcga cgaccaccgc
      481 aacggcgaaa ccctccgtgg gcgccgcaaa gacgccgcaa cctgcgacga ccactgcaac
      541 gacgaaaccc tcgacggccg ccgcaaagac gacgcaacct gcgacgaccg cgacgacaac
      601 aaagtccgcc acgacgaagc agcaggctgt agcgaagacg acggcgacga cgaagtcggc
      661 gacgacgaag aaacccgccg catcctgcgg gctcgactgc atctagcgcg ttggcgtgtc
      721 gcagctcgtc gcgaagagac gccgcctgcg accaccacga ctacaagggc cgcgacaacg
      781 acaaaatccg cgacgacgac acagcaggct cagacgaaga ccggcaccga ccgagggcga
      841 ccaccgcgaa agagcccgcc gcagcgtcaa gcgtctcgac tgcatctagc gcgttagagc
      901 gttgtctagc tcgtcgctac gtaccgccgc atcgcagtcg actgcatcta agcgcgtgag
      961 agcgctgtcg aggtcgtcgc gtcggcgtta gagcgctgtc cagcccgtcg cattgatgcg
     1021 gaggtagacg aggcgcgtcg agcccgccgt cgagctcggc gtggttgcga gaatgtgcgc
     1081 ggtgccttgc gcatcgatct tcatgtcgag cggcgtcgct tttgcgagcg cgaagctgcg
     1141 cacgtgccag ccggatgcgt cgcggtagtg atcctcgata gtggtcgagt tgttccaggg
     1201 cgggcccgcg tacacgatgt gcggctggtt gtttgcgtcg gcggccgtcg ccatcggcag
     1261 cgtcgtccac gatgcgccac ctgcgctgca cgttggtgcg tagccgtacc aggagaggtc
     1321 cgcgatcgtt tcggtggtcc acgtgccgtt gacgagacgg tgggcgcgca gccagcgctg
     1381 tgcggacgtg ccggtggaga agccgtcctc gatcgtcatg aggtcgttgc cgagcacgag
     1441 tgggttgtgc gcgatcggat agacgcacgt gccggtggtc gggacgtcgt agacggtgtc
     1501 ccacgtgaag ccgttgaggc gtttggagag cttgaggttg taggacgtgt ggttcgtgcc
     1561 tggcgtgatc gacgagagga atacgtcgcc tgtgccgctg acggcgaggc gcgttggata
     1621 gctcgtcgag aaaccggtga gcgcttcgtc gcgcgtccat gcgccgggct cgcccatttg
     1681 gtagtgcagg tcgtagaacg cggtcttgcc gacgagcgcg tgccagttgc cgcccgcgcg
     1741 cacgacgtcg tagctgtatg tcgggtttgc ggacgcgaag gtccagctgc ccgttgtggt
     1801 gtgcgtggcg gtctcgaacg tcccggatgt gctgtagatg cgatggaaga atgcgcggag
     1861 cgcgttgccg tcgaacgcga gcgagaagtg gctcggcgac atcgtcgtct gcgtttgatc
     1921 ccaggtctcg atctcgttgt tgatccacgt ggtcggtgac gaacgctctg cgaacatcgc
     1981 gtgcgcgtgc tcgcgcgaat agttcgtgac cgggtcggta aagttgatgc tgtagaacca
     2041 gctgacgtgc ggctggcctg tcgagtcgag cgcgagtgat gcggtctcga cccgttgtgg
     2101 agtggtcgtg atcgtttgcg tggtggttgt cgcggtgggc gtgcacgtgc aagacgacgg
     2161 cgtgccgctg cacgtgcagt cgacctcgac ttggcagccc gcgttgcagc cgtcgtagct
     2221 gttcgtgttg ccgtcgtcgc aggcctcgaa tgggctgcgc tttccatcgc cgcagacagg
     2281 tgccggtggg gcatcggggc gcgggtccgg caccgcggcg tccggtgacg aggacgagcc
     2341 ggcatcgggc tcgagctggc cgttggaatc gccggcgcag gccgcgacga gggagaggag
     2401 ggcgagggcg gggacggtac gtgcagacgc catgccccac gaacgcggca gggcgccaat
     2461 gtgggaagaa aatctgccta caatcggggc aggcatgcgg atcgcgtggt tagcgctcgt
     2521 gacggcggcg gcgtgcggcg gctcgggcgc tggccccgat gcggcaggcg atggcagcgg
     2581 cagcgcgccc gatgccgcgg taccgacgag gtacgtcgcg tacgtcagcg ggggcgcgaa
     2641 catcgattgg tacgacgtcg acaaggcgag cggcgtgctc acgcacgtct cgtcgatcgc
     2701 ggcgtttcgc accggcgcga actttctcgc ggtgcacggc acgcgcctct atgcggtgac
     2761 gtcgggcgat cgcgtcggcg cgtatacgat cgatccggcg acagcggggc tcacgttcat
     2821 caatgatgtg ggcaccggcg ggaccggcgt cgcgcatgtc tccgtcgatc gcacgggcgg
     2881 ctacgtgatg gttgcgaatt acggcagcgg gcacatcgat gtcgtgccgg tgcgcggcga
     2941 cggtggtctc ggcacgccac tcgcgccgat cctcgccggc atgaaggcgc accagatcat
     3001 caccgacgcg tcgaacaagt tcgtgctcgt gccatgcctc ggtgacaaca aggtcgcgca
     3061 gtacacgttc gatgcatcga gcggagcgct caccgcaaac gccacaccga agctcgatac
     3121 cgcgagcggc gcggggcctc gtcacatcgc gatcgcgccg aacgcgaagc atgcgtattt
     3181 gatcaacgag ctgaacagca cgctgtccgc gctcgccttc gatagcgcga ccgggcggct
     3241 caccgagctg cagaccgtct cgacgcgcga gtccggcgct tcaggcagca acaccggcgc
     3301 ggaaatcgtc gtgcatccaa gcggcaagtt tgtctacgcc tccaatcgcg gcgacaacaa
     3361 tatcgccgtc tttgcgatca acgcgacgac cggcatggtc accgaagtcg agcacgagag
     3421 cacgcaaggc atgacgcctc gcaacttcac gatcgatccg tcgggcacgc tgctgtacgt
     3481 cgcgaatcag aattcgggca ctgtcgttcc gctcgagatc gatccggcga ccgggcggct
     3541 cagcccgacg gcgagctcgg tcagcgtgcc aacgccacaa ttcatcggga tcgtcgagct
     3601 gcctctctag tacactgcgg ctgtggatca ggtcatgtgc gagacgtgcg cgcgaatgac
     3661 tcgcgaggac gcgctcacca tcaagacact cgagatcacc gagaccatcc acaacgcgtg
     3721 cgcgccgatc acgcattacg cggcgaagtg tcggacgtgt gggacgaagt ggctggcact
     3781 cgaggtgtac gacgaagaag gtcgccagcc gtcggagtgg agctggagcg tcgacaagtg
     3841 acgtgtatga tcgcggtgtg aggcgtttgg cgttgctgag cctcgtcgcg gcatgcgcag
     3901 tgccgccggc aggtgcgcca tcgctcgcgt cgatcacgcc ggcgtcgggc ttcacgaccg
     3961 aggaaacggc cgtgcgcctg caaggtgtct ttcacctcgc gctgtcggcg gatctcgatc
     4021 acggcacgac ggacatcgaa cagtgcagcg cgagtatcgg cacgacgcca ctcgcgaacg
     4081 tggcgtggcg cagcgagcag ctcatcgaag cgacggtacc tgcggggatg ccgcttggcg
     4141 tttacgacgt gaccgtcacg tgtggtgcgc gcgtgacgac gttgcccgag gcattcacgg
     4201 tgctgccgga aggacttccc acgcagccgt tcggcgcgcc cacgctcgtc gccgcgatcg
     4261 cgtcgccgac atctctagag gacgatccgt cgatcaccgc tgatcgcctc gagctgttct
     4321 tcaactccga tcgcccaggc agcctcggtg gcgcggacat ctacgtcacg actcgggcga
     4381 cgccgagcga tgcgtggacc acacctgtcg ccgtgcccgt cgtgaatacc gcgtactcgg
     4441 agaccacgcc gaagatcagc gccgacggcc tgacgctgta tttctcgagc gatcgtcccg
     4501 gcggcacgaa tgcgaactac gacgtctggg tttcgacacg gccgagccgc actggagcgt
     4561 ggtcggcgcc aacgtgggtc cccgagctct cctcggtggc gagcgacagc ggcgcgaatc
     4621 caacctcgtc gatgttggcg ctcgtgttgt gctcggatcg cgatggtgcc ggcgacctct
     4681 atgtcgcgaa gcgcgccgcg ctgggcgatc catggggcgc tccgagcgca ctcgtcgagg
     4741 tgaatagcgc ggagggcgac gccgacccga cgctcgcgca tggtgaccgc gtgatcgtgt
     4801 tcatctcggc gcgtcccggc ggcctcggcg gcaatgacat ctatatggcg acgcgttcgt
     4861 ccgcggcggc accgttcgac acgcccgtgc tcctccccga gctcaacagc gcgatgcagg
     4921 aagaggatcc gtacatctcc gacgacctgc gctacatcct gttcacgagc acgcgcagcg
     4981 gcgacttcga aatctacgaa gcctcgcgct agctgcggcc gagcacttcg tcgtcgtcgg
     5041 tgagcgagct cgtggcgagc gcctcgctga gatcctcgaa gcgctggtag cgcacgacgc
     5101 gcccgccact cacgcgaaag gccgacgcga cgatgcgctc gctcgtggtc tctgggatgt
     5161 gccagcgccc gcgctgctcg acgacgacct gaccgccgcg gccacagaac cagcgcagcg
     5221 gctcggcggt gaagcccgcg cgaccgagcc actggcgaag catctcgtga ccgcgcgccg
     5281 atccgcgagg cccgacgagc tcgacgtcgc gcgctgtgac tgcgcaaagc tgctcgaagt
     5341 cgcgcgcgtt gacggctacg tgccaactct cgacgacctc gagaggaaac atctgcatgt
     5401 gatcgatcta tcgcttcgcc gggcggccgt catcgcgcgt ctgcttgcag ctcatcactg
     5461 aaagaagcgg ggcgatgcgg ccgttatacg tgcatatgca ccggctactc ctgttgctac
     5521 tcgtctcggg ttgcctcacg acgcgcgtcg agacgatgtc gtggccgacg acggttcatc
     5581 ttcgattcag ccaggtctgt ccgggcagcg cggtcgaggt ctacgacgag cacgccacct
     5641 acgtcccggt cgcaatcgtc gacgagaaag tcgacgtgaa cgtcccggcg atgaggggtg
     5701 gctacgacct gcgacgcggg aagaagtcgc gcgagtccga cccggcggcg tacccggcgc
     5761 tgcggttgcg gaatggccac gaggtcgtga cggtgctgtc gcttcacgac gtaggcgcct
     5821 tcccgaagga cgccgagggc agggcggtca taccggttcg ctgctcggtg gcccaaccgg
     5881 taccagctac ctgacaggtc gtggcaccat ggaacaggcg agatgagcca acccctgttt
     5941 acgtttcgcc agctgccgac cctgtccgag atgctcggtg accgcggcat cgtgctcgct
     6001 gatgtgatgc gcgacgcagg ctttaccgag atgtcgacca cggaggtgac cgcgccgctc
     6061 tcgaaggtcc agaagctcct cgagctgtcg ggccagaagc tcgagtcgca ggcgctcggc
     6121 ctcgacctcg ccgaccggat tccgcagggc gcgtacggag tcaccgagtt tgtcgtccgc
     6181 acgtcgccca ccgttcgcca tgcgctgacg gcgatgtgcg agctcgcgcc gctgatcaat
     6241 ccggcactcg acatgcgcta tgtcgcggac cagctcggct gcgaagtgcg cttcgcgtac
     6301 gcgggcaacc gcgatgcgct aggcgagacc ctcaacgagt acaccgtcgc gtacatcgca
     6361 aagcagttcg ccgtgctttt gcagaaggcg ttgccgctca cgcgtgcgtg gttcgcgcat
     6421 ggacgcaggc aaggtggcga cgaggtctcg cggcggttcg gctgctcggt aggctttcaa
     6481 gcggccgact gcggcttcgc cgtcgccgcg gacgtgatcg cgttcacgaa cccgcagggc
     6541 aacgaagcgc tctacaagtt cctgctcgac caggcgcgca cgcagctgca gaacctcggc
     6601 aagcacgacg tgatcacgca agtcacgcgt gtgatcgagg cgcgcatctc cgacgctgat
     6661 ctcagcgcag cgacgatcgc gcaagcgctc gccctctcgc aacgcacgtt gcagcggcag
     6721 ctcaccgatg ccggcacgtc gtatcgcgat ttgctcgcgt cgattcgccg ccggcgccgc
     6781 gccgagctcg agcgcgcggg tctcgccgat gcggaaattg cgccgcgcct cgggttcgcc
     6841 agcgcgaaga cgatgcgccg ctccctcgac gacaccggcg ccgacgagcc tgcgcccgaa
     6901 gaatagaacg cgccagaaat cgttccgaac gcgccagcat tcggatctcg atggaagcga
     6961 tcggcggcgg cgcgttacgc atgcggtcga tgaagcgccg gacgttcctt cgttggctgg
     7021 cggctgcgcc ttcacttccg ctgatcggca gcgcgtgcac gtccggagat cccgaagagg
     7081 cgatgttccc gcagggtgtc gcgtcgggag atccgtcgcc cgatggcgtg ctgctgtgga
     7141 cgcgtgtcga atcgaccagc acggagcgcg tgctctacga ggtggcgagc gatcgagact
     7201 tcgtgacgat cgtgacgacc ggcgagcgcg aggtcgacga cacgacggat cacacgctgc
     7261 gcgtcgagct aacgggcctc gcatcgggca cgacgtattg gtaccgcttc accgttcgcg
     7321 gcgtgacctc gcgcgtgggc cgcacgaaga ccgcgccgga tccgactgcc gacgtcccgc
     7381 tgcggatcgc gctcgcatcg tgtcaggact tcggcggccg ttggtttcac gcatggcgcg
     7441 cgctcgtcga tcgcgatgac atcgacatcg tggtgttcat cggcgactac atctacgaga
     7501 cgatcggtca tctcggcgtc gagtggcccg cggaccgcac gatcgaattg cccgatggcc
     7561 tcgagctcga cgacgagatc aaaggctcgc gtgccgcgct cacgctcgcc gactaccgcg
     7621 cgctgtatcg ccagtaccgc agcgatcccg atctgcgcga cgcccacgcg cacttcccat
     7681 tcgtctcgct gtgggatgat cacgagttcg cgaacgattg ctggggcgat cacgcgacgg
     7741 acttcgacga ccgccatggc gacgaagcga gccccgatcg acgagcggcg gcgacgcgcg
     7801 cgtacttcga gtttcaggcg attcgccgtc cattcgatgg tgccgccgcg ttcccgaacg
     7861 acctctcgat ctaccggacg ctgcgctggg gcaagcacgc ggagctcgtg ctcctcgacg
     7921 agcgctatta ccgcgatgac gacctcgtgc ccgaaggccc gatcgatcgc gacgtcggtc
     7981 acatccagat gaacagcgca ttcggctcgc gcacgttcgt catcaaagac gcgttcgatg
     8041 cacgcgaggc tgctgcatct ccaacgccga cgatgctggg cacgccacag cgcgactggg
     8101 ccatctccgc ggtgacgagc tcgaccgcga cgtggaagct cctggccagc ccgctcgtga
     8161 tggcgcaaat ggccgtcgac ctcacgggct acgcacagct gcccgatcag ttccgcaagc
     8221 ggttctactt caagaccgac cagtgggacg gctttcgctc cgagcggcgc gcgctcctcg
     8281 aagcgtgcag cggcgtcgag aatctcgtcg tgctctcggg cgacctccac ggcttctatg
     8341 cggccgagct ctacgcagac ttcgatgcgc cgggcgagcc gatcgccgtc gagtacgcga
     8401 cgtctgcgat caccgcgccg accgtcgacg ttcagatcaa cggcgcgatc gaatcgaacg
     8461 tctttctcga ggcgctcggc ctcaaggatc tcgtccccga attcgatggc aacctcctcg
     8521 caacgaaccc gcacatcaag cacgcggact cgacgacgaa cgggctcgcg atcatcgaga
     8581 tcggcgcggc gcaggtcgac gtccgattta ttcgcgtgtc cgacgtaacg tccccgacgg
     8641 gacgcgtcat cgacgaggtg gccttccaga cgcccatcgg cagccgtcgc gtggtttact
     8701 ccgcgccatg ttcaggctga atgcactgct gattctcgga gcgctcgccg cgtgtggcgg
     8761 ggccaaaacg acaacgcaac cgacgacggg acaagaaccc gccggtccgg tggaccccaa
     8821 agaggcggcc gtcaatcagg tgcccgcttc tccggtcgcc ggcggcaaga aggccgtcgt
     8881 cgcgccgcgc ttgatcgcaa agccgctggc gggagacacg acgaagacca ccattcatcg
     8941 cctctcgaat ggcatgacgg tctatatcgc gcccgacaac aaggagccga cgattaccgc
     9001 gtacgtcgcg gtgcatgcag gcggcgcgta cgatccgcag aattcgacgg gcctcgcgca
     9061 ctacctcgag cacatgctgt tcaagggcag ctcgaagctc gggacgcagg actacgcgaa
     9121 ggagaagccg cacctcgaca agatcgcggc gctctacgac gacctgcgca agcccggcgc
     9181 cgacgccgac aagatcctca aagagatcga cagcgagacg caggctgcag cagcgttcgc
     9241 gatt
//
LOCUS       DATLBZ010000678         9720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7982277, whole genome shotgun sequence.
ACCESSION   DATLBZ010000678 DATLBZ010000000
VERSION     DATLBZ010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7982277"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VMZ53_33290"
     CDS             complement(<1..542)
                     /locus_tag="VMZ53_33290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33440.1"
                     /translation="MRRTLLLAATTVILAIAASGCAFTPGSTSDDLEVELAMHPQSNA
                     TADVTITNVSDHDVQLLSWYLPDAELQEPLFELSRDGQPVRYIGPIYKRAQPDASDYV
                     TIAPGASLTRTVNLANFYDLSTTGNYTVAVTLDNMRLRNTAGFISATVSTHIDAHPVV
                     IQGKPVRTTCTSAQLAEINAA"
     gene            complement(600..1235)
                     /locus_tag="VMZ53_33295"
     CDS             complement(600..1235)
                     /locus_tag="VMZ53_33295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HUS33441.1"
                     /translation="MLELYLIRHGQTDYSRENRFCGAIDPPLNDVGLRMAEAFGAAYA
                     KLPWTAIYSSPRTRTRQTAEALARRVEAPVLVDDGLAEIAYGEWEGMRHDEVQAKWPE
                     AYAHWSADVASRGTPGGETAFAVAARAAPVLERVRATHGSGRVLVVSHKATIRIMVCA
                     LLGLDVRLFRDRLGQGVAAVTRFELKKTGAQLVQLGDVSHLPAELRESEGT"
     gene            1338..2270
                     /locus_tag="VMZ53_33300"
     CDS             1338..2270
                     /locus_tag="VMZ53_33300"
                     /inference="COORDINATES: protein motif:HMM:NF038131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor K domain-containing protein"
                     /protein_id="HUS33442.1"
                     /translation="MRASWMVSVLAVSACNMSESDVGGVVQEVADPAPAFSIQSARFS
                     NAIASDPTMFSVAYCGSSPQAACAATPVAQVRWGTQVYATEQSGLGFAPAAALDLHYD
                     ESFDLGELTHFNFPTYAGTGASGVTLDLHLLVDSSDSSGPLFDQEIHIPFAVHETPND
                     DPETPCPYPSVMPCSDKITFGTSTFNLGAATATTNYELEIVGFVDATSSTPADGLISE
                     EYDSTSAVLRAMLREACIDVDADGTCDEVDACIGDEVACPPDPCPCDSDWKNHGEYVS
                     CVAHYTQDLVKAGSLSHQDRASIVSAAGQSSCGK"
     gene            complement(2271..3557)
                     /locus_tag="VMZ53_33305"
     CDS             complement(2271..3557)
                     /locus_tag="VMZ53_33305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS33443.1"
                     /translation="MLRRAALVAVAAFGCTQAQLPTPPARPVVKPAAASRAVDHANEA
                     IDALVGGDYAGVVNAFAPDSNPPTLSQVESAWRTQAAGLGEYRSWSQVDRTFQDGFEV
                     RILHIELERGTVQCLVSVDPGSLELRSLFITRPAQLPSYIDRAQIRDVAVTFGTPALD
                     ATLTLPSGKGPFPAVVLVHGSGPNDRNNAVGATKIFKDLAEGLASSGIAVLRYDKRTY
                     TYREQLDNSITLDDEVVLDAVAAVKLLAARTDVDASRVFVIGHSLGGLLAPEIAVRAG
                     DVAGIALLAPPGRVPWDIVRDQMRYLGAPRDTLIDTEKAVIELNVGVETGRLAGMPYS
                     YWRDWASRDGVAMVKRFARPTLILHGTRDYQVTDEDFAVWQRGLRDESHVELVTVTGA
                     NHLFIAGQGQPTPLEYKVEAHVDARVIAKLVAFVTR"
     gene            complement(3600..4574)
                     /locus_tag="VMZ53_33310"
     CDS             complement(3600..4574)
                     /locus_tag="VMZ53_33310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33444.1"
                     /translation="MRAHLLAVGLTLASCGHRPAAPTTELLRHVSDVRLPGGANRFDY
                     QDIDAATGLLVVAHMNDNSVLVLSLADGSVKKELTGIDTPRGVVIAVEVQRIFVTCMP
                     NQLVIIDSSTLAEVSRVTTGASPDGVAWDPDDQIVGVSDQGDGAISLLAMAGTGVRTQ
                     VSLGAETGNVVYDARRKHFWITTAASELVEVDPKSATVTTRIALPGCSGAHGLRLHPD
                     GRSAFVACEDGDQLARVELDATHAIATAASGADPDVLSIDAARGWLYLAAESGDVTVW
                     DIAQPGLSLVGHAHPGVGAHTVVVDPATHRTFFPLASGPVLRIMRPAS"
     gene            4601..5236
                     /locus_tag="VMZ53_33315"
     CDS             4601..5236
                     /locus_tag="VMZ53_33315"
                     /inference="COORDINATES: protein motif:HMM:NF023056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HUS33445.1"
                     /translation="MTKREIERTLLAVLRERGTATACPSDVARKLATTWRPLMPRVRE
                     VAAALQRRGVVDAYQRGRPVKIAEARGPIRLRLHEATWLFTINPRAPQAYDYHWDIDE
                     PETLLGAEDKTWGTANYFRKMIVGDELAVYMKNTGAVTGDGIYVLGRVIDVDASNREF
                     TWRPDRRRTKRLIHAPIPPEVVRELFGRGFGGPLRALKAGMERDWLQLVGR"
     gene            complement(5233..6855)
                     /locus_tag="VMZ53_33320"
     CDS             complement(5233..6855)
                     /locus_tag="VMZ53_33320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUS33446.1"
                     /translation="MRTTAAIIASLLIGGCGAYLEGDADQDDANMDESLSSLGKGVVS
                     TLRAKHPTTAGQTWGVAIDNSFASGWLLQTPPAEYWGQPSSALPVATDCGNDAACDRD
                     FHLIRCTSQADCRFGGRCSDVAATVTRPGEAARRMCVGHSDSFYDEIYKVITSAHSYV
                     DIASLEAPDGRFEAAVRNAFTFLANDNRSVRVRLLYGAVPLRGRETAEVLTTLTRDLP
                     ASSPLRISVGTYRTTVLSWNHTKLIGADGHVAIVGGHNMWTQHYLQEAPVHDISLRVT
                     GTAAVDASKFANELWQFTCHPPTNIGAVGEISALPADESVCQHPFQLTSTAAAALETG
                     TVPVITVGKLGGLDGDEGEDAILALLDSAQTQLRLSLQDIGGIGQGGPWPEHYLKSLA
                     AALGRGVDIQLVLTTLRALPGGLMVGSASYSNGWTPADVAHKLIEYADAHPDVAGDIR
                     SAVCAHLHVTSLRQGADDVWTNGAALANHAKLAIADDAAFYMGSQNWYPAQLSELGYI
                     VDDAATTRGLLDSYYSKLWSASSRVAANTCGL"
     gene            complement(6868..7122)
                     /locus_tag="VMZ53_33325"
     CDS             complement(6868..7122)
                     /locus_tag="VMZ53_33325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33447.1"
                     /translation="MKTIDIDQLSTVTGGAGASAKWDEIRSAAEPHCPETVAQFPKAP
                     ANRAQAQRIGDACLAEMGSFKASMGGRSKIQAGIDGAFPK"
     gene            complement(7255..8028)
                     /locus_tag="VMZ53_33330"
     CDS             complement(7255..8028)
                     /locus_tag="VMZ53_33330"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUS33448.1"
                     /translation="MTIKVGLCGFTMGAARYPFHFPVVEVQQTFYQPPAASVIQRWRV
                     QMPASFEFTLKAWMLITHLAKSPTYRRLRRPLTAREARDAGAFQATPIVDEAWRASVD
                     AARILRATAMLFQCPASFRPTPENIENMRSFFTRIKRPPNVRLLFEPRGPAWTTDVVR
                     PIVDELGLVHVVDPFVHTTASTDLTYFRLHGITGNRHVYTDDELRRLAAMIPQRGDTY
                     VMFNNIPRVGDAERFKPLVAGHAAGQWRTGQPRPPVHDM"
     gene            8117..9130
                     /locus_tag="VMZ53_33335"
     CDS             8117..9130
                     /locus_tag="VMZ53_33335"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HUS33449.1"
                     /translation="MEARVEPTTKPMLAALSRELPLGAYLYEPKWDGLRCMAFVRNNE
                     VDLRSRHGKPFARYFPELVSAFAAIGRDAVIDGEIVIVSETGFDFEAALNRTHPAASR
                     VARLSTETPATFIAFDLIELDGRRLGEFAERRRALVELLPERERLRPTPATEDPMVAQ
                     QWLDHVGPGIDGVVAKALDGRYQPGKRAMIKVKRARTADCVVAGIRAFVEDGQPCVSS
                     LLLGLWEGDELRHIGVCSQFTRTRREQMLRELWPLRTSFEGHPWERGFNVGRSPIGRL
                     PGSAGRWDPATMKADWIPIRPERVVEVSYDHLDGQRFRHPGQLVRWRTDREARSCTFE
                     QLP"
     gene            9106..>9720
                     /locus_tag="VMZ53_33340"
     CDS             9106..>9720
                     /locus_tag="VMZ53_33340"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33450.1"
                     /translation="MHVRAAAVSEREAIDVEGRELSLSNLDKVLYPATSFRKRDLLAY
                     YRAIAPVMLPHLADRALHLGRWPDGVDAHGWLQANCRGPAWIRTHVVTGKRGQTLKFG
                     VIDDLATLLWAGQVGTLELHPFQATITTPDEPRAVVFDLDPGPGCDLRHCAAAARAIR
                     ERIAPLQSFVKTSGVKGLHVYVPLNGGASFARAKAFAKSVAAELA"
BASE COUNT         1593 a         3060 c         3401 g         1666 t
ORIGIN      
        1 gcggcgttga tctctgcgag ctgcgcgctc gtgcaggtcg tgcgaaccgg cttgccttgg
       61 atgacgaccg ggtgcgcgtc gatatgcgtg ctcaccgtcg ccgagatgaa gcccgcggtg
      121 ttgcggaggc gcatgttgtc gagcgtgacc gcgacggtgt agttgcccgt cgtcgagagg
      181 tcgtagaaat tggcgagatt gaccgtgcgc gtgagcgagg cgccgggggc gatcgtgacg
      241 tagtcggatg cgtcgggctg cgcgcgcttg tagatcgggc cgatgtagcg caccggttgg
      301 ccatcacgcg agagctcgaa gagcggctcc tgcagctcgg cgtcgggcag gtaccacgag
      361 aggagctgca cgtcgtgatc gctcacgttc gtgatcgtga cgtcggcagt cgcgttgctc
      421 tgcgggtgca tcgcgagctc gacctccagg tcgtcggacg tgctgcccgg ggtgaacgca
      481 cagccggacg cggcgatggc aaggatgacg gtagtcgctg cgagaagaag ggtgcggcgc
      541 atgcgggagt cgtaacacgc ggcgaatccg gctcgatcag gaaagaagcg ttgcggccgt
      601 cacgtccctt cggactcgcg aagctccgcc gggagatgag acacgtctcc gagttgcaca
      661 agctgcgcac ccgtcttctt cagctcgaac cgggtcacgg cggcgacgcc ttggccgagt
      721 cgatcgcgga acaggcgcac gtcgagcccg agcagcgcgc agaccatgat gcgaatcgtg
      781 gccttgtgcg agacgacgag gacgcgcccg cttccgtggg ttgctcgcac gcgctcgagg
      841 accggggctg cacgcgccgc gacggcaaag gcggtctcgc cgccgggagt cccgcggctc
      901 gcgacgtcgg ccgaccagtg cgcgtaggcc tcgggccact tcgcctggac ttcgtcgtga
      961 cgcatgcctt cccactcgcc gtacgcgatt tcggcaaggc cgtcgtcgac gagaacgggt
     1021 gcttcgacgc gacgcgccaa tgcctccgcg gtctgccgcg tccgcgtgcg tggcgacgag
     1081 tagatggcag tccacggcag cttcgcgtac gcggcgccga aagcctctgc catgcgcaac
     1141 ccgacatcgt tgagcggcgg gtcgatcgcg ccgcagaacc ggttctcgcg tgagtagtcc
     1201 gtctgcccgt gacggatcag gtagagctcg agcacccatc gagcatggac ccatcgacgt
     1261 ggaacggtca ggtttatgcc gcaatggttg gaagaaatgt agtgacgtgg atgaccggct
     1321 ttctgtacgt tgccgggatg cgcgcttcgt ggatggtgtc ggttctcgcg gtttctgcct
     1381 gcaacatgag tgaaagcgac gtcggtggcg tcgtccagga agtcgcggat cccgcgcccg
     1441 cgttctcgat ccagtcggcg cgcttcagca atgcgatcgc gagcgacccg acgatgttca
     1501 gcgtcgcgta ctgcggatcg agcccgcagg ctgcgtgtgc ggcgacgccg gtcgcgcagg
     1561 ttcgctgggg cacgcaggtc tacgcgaccg agcagagcgg ccttgggttc gcgccggcgg
     1621 ccgcgctcga tctccactac gacgagagct tcgatctcgg cgagctgacg cacttcaact
     1681 tcccgacgta tgcaggaacg ggcgcgtccg gtgtcacgct cgatcttcat ctcctcgtcg
     1741 actcctccga ttcgagcggt ccgctgttcg atcaagagat tcacattccg ttcgccgtcc
     1801 acgagacgcc gaacgacgat ccggaaacgc cgtgtcccta tccgtcggtg atgccgtgct
     1861 cggacaagat cacgttcggc acgtcgacgt tcaacctcgg cgccgcaacg gcgacgacga
     1921 actacgagct cgagattgtc ggatttgtcg atgcgacgtc gtcgacgccc gccgacggcc
     1981 tcatctccga ggagtacgac agcacgagcg ccgtgctgcg cgcgatgctt cgcgaggcct
     2041 gcatcgacgt cgatgcggat ggtacgtgcg atgaggtcga tgcgtgcatc ggtgatgagg
     2101 tcgcctgccc gcccgatccg tgcccgtgcg atagcgattg gaagaaccac ggcgagtacg
     2161 tcagctgcgt ggcgcactac acgcaggact tggtgaaggc gggctcgctc tcgcatcaag
     2221 accgcgcgtc gatcgtctcg gccgctggcc agagcagctg cggcaagtaa ctaccgcgtc
     2281 acgaacgcga cgagcttcgc gatcacgcgc gcgtcgacgt gggcctcgac tttgtactcg
     2341 agcggcgtcg gctggccctg cccggcgatg aagagatggt tcgcgccggt gacggtgacg
     2401 agctcgacat ggctctcgtc gcgaaggccg cgctgccaga cggcgaaatc ctcgtcggtg
     2461 acttggtaat cgcgggtgcc gtgcaggatg agcgtcgggc gagcgaaccg cttcaccatg
     2521 gcgacgccat cgcgcgatgc ccagtcgcgc caatacgagt acggcatgcc ggcgagtcgg
     2581 ccggtctcga cgccgacgtt gagctcgata acggctttct cggtgtcgat gagcgtgtcg
     2641 cgcggcgcac cgaggtagcg catctggtcg cggacgatgt cccacggcac gcgcccaggc
     2701 ggcgctagca gcgcgatgcc agcgacgtcg cctgcgcgca cggcgatctc cggcgcgagc
     2761 aacccgccga ggctgtggcc gatcacgaac acgcggctgg catcgacatc ggtgcgcgcg
     2821 gcgagcagct ttacggcagc caccgcatcg aggaccacct cgtcgtcgag cgtgatcgag
     2881 ttgtcgagct gctcgcgata cgtgtacgtg cgcttgtcgt agcgcagcac ggcgatgccg
     2941 gatgacgcga gtccttcggc gaggtccttg aagatcttcg tcgcgccgac cgcgttgttg
     3001 cgatcgttcg gcccgctgcc atgaacgagc acgacggcgg gaaacgggcc cttcccgctc
     3061 ggcaacgtga gcgttgcatc cagcgcgggt gtgccgaacg tgacggcgac gtcgcgaatc
     3121 tgcgcgcggt cgatatacga cggcagctgc gcgggacgcg tgatgaacag gctgcgcagc
     3181 tcgagcgagc ccggatcgac ggagacgaga cactggacgg tgccgcgctc gagctcgatg
     3241 tggaggatgc ggacctcgaa gccgtcctga aacgtgcgat cgacctgcga ccacgagcgg
     3301 tactcgccaa gtccagcggc ttgcgtgcgc cacgcgctct cgacctgcga cagcgtcgga
     3361 ggattgctat ccggcgcaaa cgcattcacg acgccggcgt agtcgccgcc gacgagcgcg
     3421 tcgatcgctt cattcgcatg atcgacggcc cgcgacgccg cggcgggctt caccactggt
     3481 ctcgcgggcg gcgttggcag ttgcgcttgc gtgcagccaa atgcggcgac agcgacgagc
     3541 gcggcccgac ggagcatcac caagtcgaca cgtcttctcg ccgaaggttg ccagtgccgt
     3601 cagcttgccg gccgcatgat gcggagcacc ggccccgagg cgagcgggaa gaacgtgcgg
     3661 tgcgtcgcgg gatcgacgac caccgtgtgc gctccgacgc ccgggtgcgc gtgtccgacg
     3721 aggctgaggc ccggctgcgc gatgtcccac accgtcacgt cgccgctctc cgcagccaga
     3781 tagagccagc cgcgcgccgc gtcgatgctg agcacgtcgg ggtccgcgcc cgatgctgcc
     3841 gtcgcgatcg catgcgtcgc atcgagctcg acacgcgcga gctgatcgcc gtcttcgcac
     3901 gcgacgaacg cgctgcggcc atctggatga agacgaagac catgcgcgcc actgcatcct
     3961 ggaagcgcga tgcgcgtcgt caccgtcgca gacttgggat cgacttcgac gagctcgctc
     4021 gccgcggtcg tgatccagaa gtgtttgcgc ctcgcatcgt agacgacgtt tcccgtttcg
     4081 gccccgagcg aaacttgcgt gcgcacgccc gtgcccgcca tcgcgagcag cgagatcgcg
     4141 ccgtcgccct gatcggagac gcccacgatc tgatcgtccg gatcccacgc aacgccatca
     4201 ggagatgcgc ccgtcgtgac gcgcgagact tccgccagcg tcgagctgtc gataatcacg
     4261 agctggttcg gcatgcacgt gacaaagatc cgctgaacct cgaccgcgat gacgacgccg
     4321 cgcggagtgt cgataccagt aagctccttc ttcacgctcc catccgcgag tgacagcacc
     4381 agtaccgagt tgtcgttcat gtgcgcgacg acgaggagac cggtcgcggc atcgatgtcc
     4441 tgatagtcga accggttcgc tccgcccgga agccgaacat cggagacgtg gcgcagtagc
     4501 tctgtggtcg gtgctgcggg ccgatggccg cacgatgcga gcgtgaggcc gacagcgagg
     4561 agatgggcgc gcacggggcg acagagtata gaaattcgac gtgacgaagc gcgagatcga
     4621 acggacgttg ctcgcagtgc tgcgcgagcg cggtaccgca accgcatgcc cgtccgacgt
     4681 cgcgcgcaag ctcgcaacca cgtggcggcc cctgatgccg cgcgtgcgcg aggtcgcagc
     4741 tgcgctgcag cggcgaggcg tcgtcgatgc gtatcaacgc ggccggccgg tgaagatcgc
     4801 agaagcgcgc ggcccgatcc gattgcgcct gcacgaggcg acgtggctgt tcacgatcaa
     4861 tccgcgagcg ccacaggcgt acgactacca ttgggatatc gacgagccgg agacgctgct
     4921 gggcgccgag gacaagacct ggggcacggc gaactacttt cgcaagatga tcgtcggcga
     4981 cgagctcgcc gtctatatga agaacaccgg ggcggtgacc ggtgatggca tctacgtgct
     5041 cggccgggtg atcgatgtcg acgcatccaa tcgcgagttc acgtggcgac cggatcgccg
     5101 gcgcacgaag cgcctcattc acgcgccgat tccgcctgag gttgttcgcg agctgttcgg
     5161 gcgcgggttt ggcgggccgc tgcgtgcgtt gaaggcaggg atggagcgcg actggctgca
     5221 gctcgtcggg cgttagaggc cgcaggtgtt cgcggcgacg cgactggagg cggaccagag
     5281 cttcgagtag tagctgtcta ggagaccgcg cgtggtcgcg gcgtcgtcga cgatgtagcc
     5341 gagctcggag agctgcgcgg ggtaccagtt ctgcgagccc atgtagaacg ctgcgtcgtc
     5401 cgcgatcgcg agcttcgcgt ggttcgcgag cgccgcgccg ttggtccaga cgtcgtcggc
     5461 tccttggcgc agcgacgtga cgtgaaggtg cgcgcagacc gcggagcgga tgtcgccggc
     5521 gacgtcgggg tgggcgtcgg cgtactcgat gagcttgtgc gcgacgtcgg ccggcgtcca
     5581 gccattcgag tacgacgcgc tgccgaccat gaggccgccg ggaagcgcgc gcagggtcgt
     5641 gagcacgagc tggatgtcga caccgcggcc gagcgcagcg gcgagcgact tcaggtagtg
     5701 ctcgggccat gggccgcctt ggccgatacc gccgatgtct tgcagagaga gacgcagctg
     5761 cgtctgcgcg ctatcgagca gagcgaggat cgcgtcttcg ccttcgtcgc cgtccaggcc
     5821 gccgagcttg ccgacggtga tgaccggaac cgtgcccgtt tcgagcgctg cggccgcggt
     5881 cgaggtcagc tggaatggat gctggcagac gctctcgtct gctgggagcg cgctgatctc
     5941 gccgaccgcg ccgatgttgg tgggcggatg gcaggtgaat tgccagagct cgtttgcgaa
     6001 cttgcttgca tcgaccgcgg cggtgccggt gacgcgaagc gagatgtcgt ggacgggtgc
     6061 ttcttgcagg tagtgctgcg tccacatgtt gtggccaccg acgatcgcga cgtgaccgtc
     6121 ggcgccgatc agcttcgtgt ggttccacga gaggaccgtt gttcgatacg tgccgacgct
     6181 gatgcggagc ggagagctag caggcaggtc gcgcgtcagc gtagtcagca cttcggcggt
     6241 ttcgcgtccg cgaagtggga cggcgccgta gaggagacgc acacgaacac tgcggttgtc
     6301 gttggcgagg aacgtgaacg cattgcgcac cgctgcttcg aagcggccgt cgggcgcctc
     6361 gagactcgcg atgtcgacgt aggagtgggc actcgtgatg actttgtaga tctcgtcgta
     6421 gaagctgtcc gagtggccga cgcacatacg acgtgcggct tcacctggac gcgtgaccgt
     6481 cgccgcgacg tcgctgcagc ggccaccgaa gcggcagtct gcttgcgagg tgcagcgaat
     6541 gagatggaag tcgcgatcgc acgctgcgtc gttgccgcag tcggtcgcga cgggcagcgc
     6601 cgatgacggc tggccccagt attcggcagg cggagtttgc aggagccaac cggaggcgaa
     6661 cgagttgtcg atcgcgacgc cccacgtttg gccagccgtg gtcggatgct tcgcgcgcag
     6721 cgtcgagacg acgcctttgc caagggaaga cagcgactcg tccatgttgg cgtcgtcttg
     6781 atcggcgtcg ccttccagat acgcaccgca gccgccgatg aggaggctcg cgatgatggc
     6841 cgctgtcgtc cgcatgacac gctcccttca cttcgggaat gcgccgtcga tgcccgcttg
     6901 gatcttcgag cgtccgccca tcgaggcctt gaacgagccc atctcggcga ggcacgcgtc
     6961 accgatgcgc tgcgcctggg cgcggttcgc cggcgccttc ggaaactgcg cgacggtctc
     7021 ggggcagtgc ggctcggcgg ccgagcgaat ttcgtcccac ttggcagagg cgccagcgcc
     7081 tccggtgaca gtgctgagct ggtcgatgtc gatggtcttc atggctgttg tcctttcggt
     7141 tgaggacaag ctctgcagac ctggtgccgc gcgcacgctg catccctcgg gatgcgcttc
     7201 gtattcgagc gtttgcacgc gcgatgcggc atttcggcgt aactccgctg gcgttcacat
     7261 gtcgtgcacc ggcggacgcg gctgccccgt tcgccactgg ccagctgcgt ggccggctac
     7321 gagcggcttg aagcgttccg catcgccgac gcgcgggatg ttgttgaaca tcacgtacgt
     7381 gtcaccgcgc tgcgggatca tggcggcgag ccggcgcagc tcgtcgtccg tgtagacgtg
     7441 gcggttgccc gtgatgccgt gcagtcggaa gtacgtaagg tcagtcgacg ccgtcgtgtg
     7501 gacgaacgga tcgacgacgt gcaccaagcc gagctcgtcg acaatcggcc gcacgacatc
     7561 ggtcgtccac gccgggccac gcggctcgaa caacaagcga acgttcggcg ggcgcttgat
     7621 gcgcgtgaag aagctgcgca tgttttcgat gttctcgggc gtcggccgaa agctcgccgg
     7681 acattggaac agcatcgcgg tcgcgcgcag gatgcgagcc gcatccacgg acgcacgcca
     7741 cgcttcatcg acgatcggcg tggcttgaaa cgcgccggca tcgcgggcct cgcgcgcggt
     7801 cagtgggcga cgcagccggc gatacgtcgg gctcttcgcg aggtgcgtga tcagcatcca
     7861 cgccttgagc gtgaactcga atgacgccgg catctgcacg cgccagcgct ggatcaccga
     7921 cgccgccggc ggttgataga acgtctgttg gacttcgacg accgggaagt ggaatgggta
     7981 gcgcgccgcg cccatcgtga agccacatag cccgaccttg atcgtcacgt agaagtgtag
     8041 ctcgtcgacg gcgagcgacc gtcggagaga ttcgtagacg acttgcgagt ccgcgcgcag
     8101 atgtcagctt gcgtccgtgg aggcgcgtgt cgagccgacg acaaagccga tgctggcggc
     8161 gctttcgcgt gagctcccgc tcggcgcgta tctgtacgag ccgaagtggg atgggcttcg
     8221 ctgcatggcg ttcgtgcgga acaacgaggt cgatctgcgc agccgacacg gcaagccatt
     8281 cgcccggtac tttcccgagc tggtatcggc gttcgctgcg atcggacgcg acgcggtcat
     8341 cgacggcgaa atcgtgatcg tcagcgagac tggctttgat ttcgaagccg cgctgaatcg
     8401 cacgcatcct gcggcatcac gcgtggcgcg cctgagcacg gagacacccg cgacgttcat
     8461 cgcgttcgat ctcatcgagc tcgacggccg gcgtctcgga gaatttgccg aacgcaggcg
     8521 cgcgctcgtc gagcttctgc cggagcgcga gcgtctacga ccgacaccgg cgacggaaga
     8581 tccgatggtc gcgcagcagt ggctcgatca cgtcggcccg ggcatcgacg gcgtcgtcgc
     8641 gaaagcactc gatggccggt atcagccggg caagcgcgcc atgatcaagg tgaagcgagc
     8701 gcgcactgcg gactgcgtcg tcgctggcat tcgcgcgttc gtcgaggatg ggcaaccgtg
     8761 cgtgtcgtcg ctgctgctgg gcctgtggga gggtgacgag ctccgtcaca tcggggtgtg
     8821 ttcgcagttc acgaggacgc gtcgcgagca aatgcttcgc gagctgtggc cgctgcgcac
     8881 cagcttcgag ggacatccgt gggagcgcgg cttcaatgtc ggccgcagtc cgatcggcag
     8941 gctgccgggc tcggctggac gatgggatcc ggcgacgatg aaagcggatt ggatcccgat
     9001 tcggcccgag cgcgtcgtcg aggtttcgta cgatcacctc gatgggcagc gcttccgcca
     9061 tcccgggcag ctcgtgcggt ggcgaaccga tcgggaggcg cgctcgtgca cgttcgagca
     9121 gctgccgtaa gcgagcgcga ggcgatcgac gtcgagggcc gcgagctctc gctgtcgaat
     9181 ctcgacaagg tgctctatcc cgcgacgagc tttcgcaagc gcgatctact cgcgtactac
     9241 cgcgccatcg cgccggtgat gctgccgcac ttggccgacc gcgcgctgca cttgggccgc
     9301 tggcccgacg gcgtcgacgc acatggatgg ttgcaggcga actgtcgagg acctgcatgg
     9361 attcgcacgc acgtcgtgac cggcaagcgc ggccagacgc tgaagttcgg cgtcatcgac
     9421 gacctcgcga cactcttgtg ggctggccaa gtcgggacgc tcgagctgca tccgtttcag
     9481 gcgaccatca ctacgcccga cgagccacgc gccgtcgtct tcgatctcga tccgggaccc
     9541 ggctgcgatc tacggcactg cgctgctgct gcgcgggcca ttcgcgagcg gatcgcgccg
     9601 ctgcagagct tcgtcaagac ctccggcgtg aagggactgc acgtctacgt gccgctgaac
     9661 ggcggcgcca gctttgctcg cgcgaaggcg ttcgcgaagt cggtcgcggc cgagcttgca
//
LOCUS       DATLBZ010000679         5932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6495281, whole genome shotgun sequence.
ACCESSION   DATLBZ010000679 DATLBZ010000000
VERSION     DATLBZ010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6495281"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VMZ53_33345"
     CDS             <1..345
                     /locus_tag="VMZ53_33345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33451.1"
                     /translation="GQYTSMCESESWSPERRSCILGAGDLINAHLCAGHVAPSDTTPA
                     VIPANLACGVVGPTIATTLQSAGFHDDVADLGDQIAAACEVGNWTIDLRTCLGEAVSV
                     DALKECIAHVEP"
     gene            361..1263
                     /locus_tag="VMZ53_33350"
     CDS             361..1263
                     /locus_tag="VMZ53_33350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33452.1"
                     /translation="MADPSITRVTLFVPGAESLRGARLGKSVEIEWVANDGHFGDAFS
                     FGTVAPKVVKQIDNAPGALVLHFTSDLRESRSEIVAAVAALRDDGALAVRLEQSKMGW
                     DVQRWLELFSSEEATSWHRGAVVFVGGNEVMQSCGMHAFSLPDVRLSHEPRVKPSTLQ
                     ELGTLLNLYQLVEDPVIRSGQTFSPDHETPRRVLERWPDVEYPADHPCHNPYGVWRVG
                     EAGGTARAMGELAPTFVPTLRALLAALESKAGRHLTRAQVEAARDGGTCIAMKPKDAQ
                     MLERQRGYADLDPELVWEQWKLVR"
     gene            complement(1260..1937)
                     /locus_tag="VMZ53_33355"
     CDS             complement(1260..1937)
                     /locus_tag="VMZ53_33355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33453.1"
                     /translation="MIARPLTLPESVASLGADAGGNHDLSAIGAAPVVGYGITEKIDV
                     QVPYAFAVHDLQARGSVAVDAGYAVVRGAFDGKLEVIERIRAGYDTLGTSSMPIQLGL
                     HAQYNVTPWLAVISGTPGSQQLRISLADNAEMKKPVDISLPLGIGVQPTETLYLQLDA
                     KLLQVALSESDNLVFGRDIAPLALTVVYNVITALDVQAAIATDFANGPADTLAFLVGA
                     RFYAGEL"
     gene            2161..3585
                     /locus_tag="VMZ53_33360"
     CDS             2161..3585
                     /locus_tag="VMZ53_33360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HUS33454.1"
                     /translation="MEPTSVEQRLANLEAYITNSAPHGGTLASTAGPGHNGWMMIATA
                     LVLFMTLPGLGLFYGGLVRRKNVLSVLAQCFGCAGLVTILWWAIGYSLVFAKGNAVIG
                     GFDFAMFNGVTATPNGDYAAWVSQNVFAMFQMMFAIITPALIVGAIAERMKYSAVIAF
                     ITLWMFVVYFPLAHMVWGIDGLMNGVWNAKASIKAVDFAGGTVVHMSSGWSALVLCII
                     LGKRTGFGKRAFMPHSLVLTVVGTGMLWVGWYGFNAGSAVAADGIAANAFVTTTLAAA
                     VAGAVWPALEWSTRGKPSVLGFCSGVVAGLVVITPCAGYVSASSAILIGLLAGVVPYL
                     ACTKLKAIFKYDDALDTFGVHGVGGTLGCILAGVFASPEINPNLSTNLKDLVGNGLWL
                     QQLEAMGITLALSIGGTAAIAYAIKAVIGLRPSVEAEEAGLDDADHGEAGYHGDESGH
                     GGLEELELPSLEPSRVLDATSSPS"
     gene            3687..4028
                     /locus_tag="VMZ53_33365"
     CDS             3687..4028
                     /locus_tag="VMZ53_33365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33455.1"
                     /translation="MPPPGTTLCVTCGRNLAFSGYPQQPAMAPYGGSYIYPSRGSTIL
                     VLGILSLVVCSILGPIAWSMGNEEQRRIAAGQAPPDGNVSAGRICGMISSILLILGGC
                     FILFALAIAPR"
     gene            3989..4414
                     /locus_tag="VMZ53_33370"
     CDS             3989..4414
                     /locus_tag="VMZ53_33370"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HUS33456.1"
                     /translation="MLHPVRVGDRAALAQHSLAEVAARLLAAAMVIAALALLLVGALG
                     TPQAQAAVGEGGPSCIMKWTTGIDCPFCGMTRATIAMGAGDWHRAFALHPLAPVVLLL
                     VLGMLGIVAAGKTRTLLAGPRPWMLLSTFIAIWVVRLAV"
     gene            4470..5864
                     /locus_tag="VMZ53_33375"
     CDS             4470..5864
                     /locus_tag="VMZ53_33375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33457.1"
                     /translation="MGLPACWTSHSRDPNPPPPPPPPHDDGYVAHVETVDTVDHRPQD
                     PWQWQANPAAAAPQRCTPETVCGRSDTKHGPTGCGPTGDHLESFAGNYQKMTITKLSW
                     AANPAELRPFTLDLAETASYQRTVTVSSGVDHYCCYSQCTPSRPASQLPVVPAGMQLV
                     TNCSAAPAQVANPHADNADCPATLGRDPYVSGTNSQCCYGSIEPIPPPPPVRHYRGRA
                     ARVDGEVVMAPVRGGATWRADELRPTVADLSSDTRAALRDAWQLAAQMEHASIAAFSA
                     LSLRLMSLGAPADLIARTHEAALDEIRHTQIAFALASAYAAMPLEPACFDAAARVPTA
                     STLSELARETFLDGCIEETSAAVDASLAANDARDPVIAAALRSIADDEARHAALAWSI
                     VGWCVRAEPAIARDLRALLDELVPAVRVSAPDALDLAGHGIRSPADSARTRDAVLRDV
                     VAPCLLALEQELAG"
BASE COUNT          916 a         1993 c         1969 g         1054 t
ORIGIN      
        1 ggtcaataca cctcgatgtg cgagtccgaa tcgtggtcgc ccgagcgtcg ctcgtgcatt
       61 ctcggtgcgg gcgatctgat caacgcgcat ttgtgcgccg gtcacgtcgc gccgagtgac
      121 acgactccgg cggtgattcc ggcgaatctg gcgtgcggcg tcgtcggccc cacgatcgcg
      181 acgacgctgc agtcggctgg cttccacgac gacgtcgctg acctcggcga tcagattgcc
      241 gcggcgtgcg aggtcggcaa ctggaccatc gacctgcgca cgtgtctcgg cgaagctgtg
      301 tccgtcgatg cgctcaaaga gtgcatcgct cacgtcgagc cgtgacgcta tgctcgtcgc
      361 gtggccgatc cctcgatcac gcgcgtgaca ctctttgtcc ctggagcgga gtccctccgc
      421 ggcgccaggc tcggcaagtc cgtcgagatc gagtgggtcg caaacgatgg ccacttcggc
      481 gatgcgttct cgtttggcac ggtggcgccg aaagtcgtca agcagatcga caatgcgccc
      541 ggtgcgctcg tgctgcactt caccagcgat ctgcgcgagt ctcgctcgga gattgtcgct
      601 gcggttgccg cgctccgaga tgacggcgcg cttgccgtgc gcctcgagca gtcgaagatg
      661 ggctgggatg ttcagcgctg gctcgagctg ttctcgtcgg aagaggcaac gtcctggcac
      721 cggggagcgg tcgtgttcgt cggcggtaac gaagtgatgc agtcgtgtgg catgcacgcg
      781 ttctcgctgc ctgacgtgcg cctgtcgcac gaaccgcggg tcaagccgag cacgctgcaa
      841 gagctcggca cgctcctcaa cctgtatcag ctcgtcgaag atccggtgat ccgctcgggc
      901 cagacgttct cgccggatca cgagacaccg cggcgcgtcc tggagcgctg gcccgatgtc
      961 gaatatccag ccgaccaccc ctgccacaat ccgtacggcg tctggcgagt cggcgaggct
     1021 ggtggcaccg cgcgtgcgat gggcgagctc gcgccgacgt tcgttcccac tctccgcgca
     1081 ttactcgctg cgctcgagag caaggccggc agacacctca cgagggcgca agtcgaagca
     1141 gcgcgcgacg gcggcacatg tatcgcgatg aagccgaagg atgcgcagat gctcgagcgc
     1201 cagcgcggct acgcagatct cgatcccgag ctcgtctggg agcagtggaa gctggtgcgc
     1261 tagagctcgc ctgcgtaaaa ccgcgcgccg acgaggaacg cgagcgtatc cgctggaccg
     1321 ttcgcgaaat ccgtcgcgat cgccgcctgg acgtcgagcg cggtgatcac gttgtagacg
     1381 accgtcagcg cgagcggcgc gatgtcgcga ccaaagacga ggttgtccga ttctgagagc
     1441 gcgacttgca gcagcttcgc gtcgagctgc agatacagcg tctccgtcgg ttgcacgcca
     1501 atgccgagcg gcaggctgat atcgacgggc ttcttcatct cggcgttgtc cgcgagcgag
     1561 atgcgcagct gctggctccc gggcgtgccc gagatgaccg cgagccaggg cgtcacgttg
     1621 tattgcgcgt gcaggccgag ctggatcggc atcgaggacg tgccaagcgt gtcgtagccg
     1681 gcgcgaatgc gttcgatcac ctcgagcttg ccatcgaacg ctccgcgcac gacggcgtat
     1741 cccgcgtcga ccgcgacgct gccgcgagct tgcagatcgt gcacggcgaa tgcgtagggg
     1801 acctgcacgt cgatcttctc ggtaatgccg taaccgacga caggcgctgc gccgatcgcg
     1861 ctcagatcgt ggttgccacc tgcgtccgcg ccgagcgaag caacgctctc cggcaacgtg
     1921 agcggtcgtg cgatcacggc tcgcggatag cgaggaccat ccgcgtacgc aggcacgctg
     1981 aagaggacga cgagggaaca acaacaacga agcagcaagc tgttgggtct ccgcatgggc
     2041 aacgacactg cgcgcactca cgtttcgcgc acgttgcttc ggcgtttctc cttgctgcgg
     2101 cgcgaagctt tagccagcgc gacacgcgtt ggaaacaatc agctcgttac cgtcatctgc
     2161 atggaaccga cctccgtcga gcaacgactc gcgaatctcg aggcgtacat cacgaacagc
     2221 gcgcctcatg gtggaaccct cgcgtcgacc gctgggcctg gccacaacgg ctggatgatg
     2281 atcgcgaccg cgctcgtgtt gttcatgacg ctgcccgggc tggggctctt ctacggcggc
     2341 ctcgtccggc gcaagaacgt gctctccgtc ctcgcgcaat gcttcggctg cgcgggtctc
     2401 gtcaccatcc tgtggtgggc catcggctac agcctcgtgt ttgcgaaggg caacgcggtg
     2461 atcggcgggt tcgacttcgc gatgttcaac ggtgtcacgg cgacgccgaa cggcgactac
     2521 gccgcgtggg tgtctcagaa cgtgttcgcg atgtttcaga tgatgttcgc gatcatcacg
     2581 ccggcgctca tcgtcggtgc catcgccgag cgaatgaagt actcggcggt gatcgcgttc
     2641 atcacgcttt ggatgttcgt cgtctacttc ccgctcgcgc acatggtgtg gggcatcgac
     2701 gggctcatga acggcgtgtg gaatgcgaag gcctcgatca aggcagtcga tttcgcgggc
     2761 gggaccgtcg tgcacatgtc gtcgggatgg tcggccctcg tgctctgcat catcctcggc
     2821 aagcggacgg gcttcggcaa gcgcgcgttc atgccgcaca gcctggtgct caccgtcgtc
     2881 ggcaccggca tgctgtgggt cggctggtac ggcttcaacg cgggcagcgc ggtcgcagcc
     2941 gacggcatcg ccgcgaacgc attcgtcacg acgacgctcg ccgccgcagt cgccggcgcc
     3001 gtgtggcctg cgctcgagtg gtcgacgcgc ggtaaaccga gcgtgctcgg attctgctcg
     3061 ggcgttgtcg cgggcctcgt cgtcatcacg ccgtgcgcgg gctacgtctc ggcgagcagc
     3121 gcgatcttga tcggcctgtt ggcgggcgtc gtgccgtacc tcgcgtgcac gaagctcaag
     3181 gcgatcttca aatacgacga cgcgctcgac acgttcggcg tccacggcgt cggcggcaca
     3241 ctcggctgca ttctcgccgg cgtcttcgcc tcccccgaga tcaatccaaa tctcagcacg
     3301 aacctgaagg atctcgtcgg taacggcctc tggctgcagc agctcgaagc catgggcatc
     3361 acgctggcgc tctcgatcgg cggcacggcg gccatcgcgt acgcgatcaa agcggtgatc
     3421 ggcctgcgcc cgagcgtcga ggcagaagaa gccggactcg acgacgccga ccacggcgaa
     3481 gcgggctacc acggcgacga atctggccac ggcggcctcg aggagctcga gctcccctcg
     3541 ctggagccca gccgcgtcct cgacgccacc agctcgccgt cgtaacgctc ctgtcgatcc
     3601 tccttgcagc ggggcctttc gtatcgtgta ctgagggctc gaacgtattc gggaggaccg
     3661 gagtgatttg tccgaactgt ggaacgatgc ccccgcctgg caccacgctc tgcgtgacgt
     3721 gtggccgcaa cctcgctttc agcggctacc cgcagcagcc agcgatggcg ccgtacggcg
     3781 gtagctacat ctatccgtcg cgcggcagca cgatcctcgt gctcggaatc ttgtcgctcg
     3841 tcgtctgctc gatcctcggg ccgatcgcgt ggtcgatggg caacgaagag cagcgccgca
     3901 tcgccgcggg ccaagcgcct cccgacggca acgtctcggc gggtcgcatt tgcggcatga
     3961 tctcgtcgat cctcctcatc ctcggcggat gcttcatcct gttcgcgttg gcgatcgcgc
     4021 cgcgctagcg cagcactcgc tcgccgaggt tgccgcgagg cttctcgcgg cagcgatggt
     4081 gatcgccgcg ctcgcgctcc tgctcgtcgg tgcgctgggc actccgcagg cgcaggccgc
     4141 ggtgggcgag ggtgggccgt cgtgcatcat gaagtggacg acgggcatcg actgcccgtt
     4201 ctgcggcatg acgcgcgcga cgatcgcgat gggcgccgga gattggcacc gcgcgttcgc
     4261 gctgcatccg ctcgcaccgg tcgtgctgct gctggtgctc gggatgctcg gtattgtcgc
     4321 cgccggcaag acgcggacgt tgctcgcggg cccgcggccg tggatgctgc tgtcgacatt
     4381 catcgcgatc tgggtcgttc gcctcgccgt ataatcggcg tgggggacac atgcagcttc
     4441 gccgtttgcg tcatcagatc ttgctggcga tgggattgcc cgcgtgttgg acgtcgcact
     4501 cacgcgatcc gaacccgcca ccgccaccgc ctccgccgca cgacgatgga tacgtcgcgc
     4561 acgtcgaaac ggtcgatacc gtcgatcaca ggccgcagga tccctggcag tggcaggcca
     4621 accccgcagc cgccgcaccg cagcgctgca cgcccgaaac cgtctgcggg cgcagcgaca
     4681 cgaagcatgg tcctaccggc tgcggtccaa cgggcgatca cctcgagagc ttcgcgggga
     4741 actaccagaa gatgacgatc acgaagttgt cgtgggccgc aaaccccgcg gagcttcggc
     4801 cgttcacgct cgacctcgca gagaccgctt cgtatcagcg cacggtcact gtctcgtcgg
     4861 gcgtcgatca ttactgctgc tactcgcagt gcacgccctc gcggcccgcg tcgcaactgc
     4921 cggtcgtccc tgcaggcatg cagctcgtca cgaactgcag tgcggcgccc gcgcaggtcg
     4981 cgaatccgca cgctgataat gccgattgcc cagcgacgct cggccgcgat ccctacgtct
     5041 cgggaaccaa ctcgcagtgc tgctacggct cgatcgaacc gattccgcca ccgccgcccg
     5101 tgcgtcacta tcgagggcgt gccgcccgcg tcgacggcga ggttgtcatg gcacccgtgc
     5161 gtggcggtgc aacgtggcgc gccgacgagc ttcggccgac cgtcgcggat ctgtcgtccg
     5221 atacacgtgc ggcgctgcgc gatgcttggc aactcgcggc gcagatggag cacgcatcga
     5281 tcgcggcatt ctccgcgctg tcgctgcggc tcatgtcgct cggcgcgcct gccgatctga
     5341 tcgcgcgcac gcacgaagca gcgctcgacg agattcgcca tacgcagatc gcgttcgcgc
     5401 tcgcgtcggc gtacgccgcg atgccgctgg agcctgcatg cttcgatgcg gctgcgcgcg
     5461 tgccgaccgc cagcacgctg tccgagctcg cgcgcgagac gtttctcgat ggctgcatcg
     5521 aggagacgtc cgcggcggtg gacgcctcgc tcgccgcgaa cgacgctcgc gatccagtga
     5581 tcgcggccgc gctgcgatcg atcgcagacg acgaggcccg ccacgccgcg cttgcctggt
     5641 cgatcgtcgg ctggtgcgtg cgtgccgagc cggcaattgc acgcgatctt cgcgcgctcc
     5701 tcgacgagct cgtgcccgcg gtgcgtgtct ccgccccgga cgcgctggac ctcgcaggcc
     5761 acggcattcg gtcgcctgct gactcggctc gcacccgtga cgcagtgctt cgcgacgtcg
     5821 tcgcgccgtg tctgctcgcg ctcgaacaag agctcgcggg gtagtccggc ggtgagggcg
     5881 cgcgccgtca cccggcggcg gcgccagccc tcagagcgag cccggtttgc cg
//
LOCUS       DATLBZ010000680         9660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6500985, whole genome shotgun sequence.
ACCESSION   DATLBZ010000680 DATLBZ010000000
VERSION     DATLBZ010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6500985"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VMZ53_33380"
     CDS             complement(<1..938)
                     /locus_tag="VMZ53_33380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33458.1"
                     /translation="MCGYRLQAAKPAPAAANGGAAGQPRAQSPNPAGGSSGKTPPPQG
                     AKPRPRPMGHKAKGDEIYIGQTLNNRFKVESKIGEGGFGAVYRGIQLATGRKVALKLL
                     HPEMTKDENLVARFRREGMVLCNLRDAHTITTYDFDQTPDGTLYIAMELLEGKSLHQI
                     FHEQAPLDWKRMFKILIEMCSSLAEAHQQGIVHRDLKPENVYLESRPGNPEFVKILDF
                     GIAKVMRGDSIDPQSPQLTATGQTLGTLEYMSPEQLMGKQLDGRSDVYALGVVAYEMI
                     TGRLPFPDAKGPAGLITAQLKQTPQPPSVANPKASLP"
     gene            complement(1058..1519)
                     /locus_tag="VMZ53_33385"
     CDS             complement(1058..1519)
                     /locus_tag="VMZ53_33385"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS33459.1"
                     /translation="MRVVIDHIAGSRAGQRQEFSAGTRVRFGRHPDCEVSFDPHRDID
                     ASSRHAELRVARADATDPLSQGWVLVDLGSSNGTYVEGHRVTETHVSQNVPISVEFGP
                     GGPRIRLFVGDENAIEGLPPAPIEKRRRPWLLPAIACAIGLVLLGLLLTNC"
     gene            complement(1535..2674)
                     /locus_tag="VMZ53_33390"
     CDS             complement(1535..2674)
                     /locus_tag="VMZ53_33390"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HUS33460.1"
                     /translation="MTELARLEITVAAATDVGTVREHNEDSFLVGNLDKGVKLDLAEP
                     FAAKADRGPLMIVCDGMGGVEGGEVASDLAAQSIWREMQATPGTRDPEVFARLLRRAT
                     RVANHDVHSMSKREPGLKGMGTTVSAAGIVNDRLVIATVGDSRAYVLRDGALVQVTRD
                     QSLSSALIAAGHDEEVAERAGSAILQALGVNDDVEPSLSIIELRRGDRVLLCSDGLHG
                     LVGDPALALLLGAPHTVGESVKLLVTAARAAGGSDNITAVVIDVGGERLAAPKSEDDL
                     PTFREFDPQKEGDPALTSTSYVARRLAAKIGLPTSSIPPSIPVTGQHAILRSRLGMRV
                     PATALPIDDVDGPARKRLQQTPIAWWWYVVLGAFACALAWIAGGR"
     gene            complement(2671..4107)
                     /locus_tag="VMZ53_33395"
     CDS             complement(2671..4107)
                     /locus_tag="VMZ53_33395"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS33461.1"
                     /translation="MLRCSACGHQSPPGSSFCLNCGTALSAATASTQVPGGGLPGVTI
                     CTACRGENPPGMKFCRNCGATLGTTPGSPSFAPGGAPMMAPPPGGPIAAPVPFPPPNP
                     AFAPPTGPNASPPLVVPLATSAGGRPSQGGAATITCPRCGTATPTSFAYCQQCGLHMQ
                     PIAPTDPGAGSGNSPSRIRPPTNQPPMSGPPLVGGALGIMNKAPMPQPGVDPQAATLA
                     TPGGGGAAAMVGAIAVGRAHTPSAPPPVAIGTNAPPTRPSSATAGGAWGAAVLVNRDG
                     TDGQRFALTSEDTIVGRAGADIAFDDDRFLAKQHARIAKGPDGTIKIHPIDTTNGVFK
                     KADASVELSDGMIVLVGREVLRFERVTPEERTIHPLVRHGVALFGSPPRDPWGRLLQL
                     IPSGGYRDVRHLVGEEVVLGREEGDIVFRDDAFMSRRHAAITWDGKRAMLTDLGSSNG
                     TFFRLSGPMTIKNGDHVRMGDQLLRIER"
     gene            4210..5286
                     /locus_tag="VMZ53_33400"
     CDS             4210..5286
                     /locus_tag="VMZ53_33400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33462.1"
                     /translation="MAPKLANVLGKFLVVLLLLQAACAETSIRTAPFRSRPDAADPGS
                     LAGPFTGRVLDATTKAPIAGALIYAAWTLERGSALVEPAGSREVVGSTDAGGYYKINQ
                     LGSLPRGSRVTEFTLLVYKRGYVAYRSDRRFYDLGPRMDFAQSGNQVLLERWRNELSH
                     ARHLRFVGSGTAVAALTSWELADASAELEGKRPSGDDLRPGSSEGSYVVAAQLLTDND
                     IKARTKYDGQFETGPLSDEPDTATYSSQHFKALGRPESWDVALRVWRLDAAKAQERYE
                     ELLTQLPGVTEKDEIGSRSFVAVENAQGNEIRGVGFLDGTRGIVVLLTCGASQCTSID
                     DAAFLGASIYNKLKQIVPSPGGGQ"
     gene            5283..6665
                     /locus_tag="VMZ53_33405"
     CDS             5283..6665
                     /locus_tag="VMZ53_33405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33463.1"
                     /translation="MKKAAALLFVALFAGEAREAHAWEAETTQAGLAEQAALSSRLHK
                     RLVVLGFEGGLFEPLTVPPADAPALIETLHLLSPSHGCVPDARGRQTALAWIAAGAAI
                     ADVPAKLGANHFFDPSTKQGWRKPSRGVLAGLSDVVREAVGRASVPAKGVPAIDWVTD
                     KNNPLNLTGFLDQYAKAVSAATPGERSRAMAGALVAAGAMLHTLGDLGAPSRVRGDEA
                     AHFEQLGGGPDDLGSRFERVAALAFGRLGIPAPSRVITREHLRDYFSAADGSGLADMT
                     ATGYFSPNTLPGSTRVGANTKPKLARPLPALPTKLNLMAATREDGTTLRNPAGVCLAR
                     YHVDHGVVTFSLDDECMLEEVTAILPEVAAFETGLLDFLLRGELTVTAAADLAISANG
                     LGAGTLELFVEDASGVRKSVGKQDVAAGKLDVHVALPADGTRVVALFRGVDSAGEPLV
                     AIGAAPLAAH"
     gene            6704..7651
                     /locus_tag="VMZ53_33410"
     CDS             6704..7651
                     /locus_tag="VMZ53_33410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33464.1"
                     /translation="MAKKREEGDTLDRVFALIDEAPPGLHDLDEPSEDLVPGLPAPLI
                     ELYARCDGARIFVEAIEILAAREVHFEHGRWKFAVIDGADVWIDHRGKIWRTEESIED
                     DVCDGTRLDRWLAGQLDSLALVFDGDGEYAEDVFDEDGEIMAIIREKQYRAQLKRDAA
                     APGPRWRLAHVLLEQGAVEDSRNELEQVVADDPAFFWAWLDLARVSERLGEIGGAIDE
                     ARMAAEAAEGVQHAQAGYFWSQVARLAVRAGDDMVRAQAATKASLLAPELKRAQMEGA
                     RECIDAGDKASAKGLLDLLRAVWPRDLEVLELYRRVETI"
     gene            7770..8258
                     /locus_tag="VMZ53_33415"
     CDS             7770..8258
                     /locus_tag="VMZ53_33415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33465.1"
                     /translation="MRRAFILDLSTHGQAAKDLSVGGVFIENASVDFDDECHIILRAG
                     SEDLTVTARAVQILEGGAGFQIEGMNRELRERIATLVDLAMHSDLDRKKTLPHSLAKL
                     EAAKRSAAGSIPPETRRSARVAAGSISPILGLAPTQQDDALGDKIRAAKLALDREKET
                     DD"
     gene            complement(8255..9559)
                     /locus_tag="VMZ53_33420"
     CDS             complement(8255..9559)
                     /locus_tag="VMZ53_33420"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HUS33466.1"
                     /translation="MRTPTLAAFTILLSVSAAHAGGFGIPDIGVRRTAMGAVIGRPDE
                     GAAIYHNPAGLILQPGWHLYLSSGLAMIRSEFQLHSWAESDRFLGVSADMDGYYEPVR
                     PARAMGVIPMLALTGEIIPGKLVLGSALYVGNAQGAAFNDDDVTHYHLIDGYVVAPQA
                     VVAGSYRVTESVALGASAGVMNLRIHGKRDVYPIVNGTDVSKTIGSRPELVLDGSGWA
                     PTWMLAAYGRPHPRLTWGATLTGRVDATMRGPVQITYSDDAETPGDTLVGTQSTTQML
                     PWAFMAGASFDLTPQIEVGGEGRYWLYRQYKKQHTDVVGIFLVRELETMKNYNDSWQV
                     SGGVRVHDVRQLRGIELMAGSHFDRTPAPTATVTLDQPTFRHIGLHSGARWTTGRYRL
                     GASYLRYFYLIPEVTDSTTTPQSNFRGRGAMHIFTVSLEAAL"
BASE COUNT         1624 a         3116 c         3258 g         1662 t
ORIGIN      
        1 ggaaggcttg ccttcgggtt ggccaccgac ggcggttgcg gcgtttgctt cagctgcgcg
       61 gtgatgagac cggccggccc cttcgcatcc gggaacggca ggcgtcccgt gatcatctcg
      121 tacgcgacga cgccgagcgc gtagacatcg ctgcgaccgt cgagctgctt gcccatcagc
      181 tgctcgggcg acatgtactc gagcgtgccg agcgtctgtc ccgtcgcggt gagctgcggc
      241 gactgcggat cgatcgaatc tccgcgcatc accttggcga taccgaagtc gaggatcttc
      301 acgaactcgg gattgccggg acgactctcg agataaacgt tctcgggctt cagatcgcga
      361 tgaacgatgc cctgctgatg cgcttcggcg agcgagctgc acatctcgat gaggatcttg
      421 aacatgcgct tccaatcgag cggcgcttgc tcgtggaaga tctggtgcag cgacttgccc
      481 tcgaggagct ccatcgcgat gtagagcgtg ccgtcgggcg tttgatcgaa gtcgtacgtc
      541 gtgatcgtgt gcgcgtcgcg caggttgcag agcaccatgc cttcgcgacg aaagcgtgcg
      601 acgaggtttt cgtccttcgt catctccggg tggagcaact tgagcgcgac cttgcggccc
      661 gtcgcgagct ggatgccgcg atacaccgcg ccgaatccgc cttcgccgat cttcgactcg
      721 accttgaagc gattgttgag cgtctggccg atgtagatct catcgccctt cgccttgtgc
      781 cccatcggac gcgggcgcgg cttcgcacct tgcggcggcg gcgtcttgcc actgctcccg
      841 ccagcgggat tcggcgactg cgcgcgcggt tgtcctgcag ctccgccgtt cgctgccgca
      901 ggcgcgggct tcgcggcctg gagccggtag ccgcacacgc cacaaaagcg cgctgtcggc
      961 tccagcggcg aactacacga agggcagttc atcggtccca gggcaaattc tcgcagaaga
     1021 accgaaggtt cgcacctatg ttcccgcctt tttatggcta gcagttagtc aggagaagcc
     1081 cgagtaacac cagcccgatc gcgcaggcga tcgcaggcag cagccacgga cgacgccgct
     1141 tctcgattgg tgcaggtggc agcccttcga tggcgttttc gtctccaacg aagaggcgaa
     1201 ttcgcggtcc gcccgggccg aattcaacgc tgatcggcac gttctggctc acgtgtgtct
     1261 cggtgacacg gtgaccttcg acgtaggtgc cgttcgagct gcccaagtcg acgagaaccc
     1321 agccctgcga caacggatcc gtcgcatcgg cgcgcgcgac acgaagctcg gcatgccgag
     1381 agctcgcgtc gatatcgcga tgtggatcga acgagacttc gcagtccgga tggcgtccaa
     1441 accggactcg cgtcccggct gaaaactcct gacgctgtcc ggcacggcta cccgcgatgt
     1501 ggtcgatcac cacccgcatt cttcaggcgg tagtctaccg cccgccagcg atccacgcga
     1561 gggcacaggc gaaagctccg agcacgacgt accaccacca tgcgatcggc gtctgctgga
     1621 gccgcttgcg tgccgggcca tcgacgtcgt cgatggggag cgccgttgcc ggaacccgca
     1681 tgccgagccg cgaccgcagg attgcatgtt gcccggtcac cgggatcgac ggcgggatgg
     1741 acgacgtcgg caggccgatc ttcgcggcga gccgccgagc gacatacgac gtcgaggtca
     1801 gcgcggggtc gccctctttt tgcggatcga attcgcggaa cgtcggtagg tcatcttcgg
     1861 acttcggcgc tgcgaggcgt tcgccaccaa cgtcgatcac gacggccgtg atgttgtcgc
     1921 tgccgccggc tgcgcgtgct gcggtgacga gaagcttgac cgattcgccg acggtgtgcg
     1981 gcgcaccgag cagcagcgcg agcgccggat cgccgacgag gccgtgcaga ccgtcgctgc
     2041 agagcagcac gcgatcgccg cgacgcagct cgatgatcga cagtgacggc tcgacatcat
     2101 cattgacgcc gagtgcctgg aggatggcgg agcccgcccg ctcggcgact tcttcgtcgt
     2161 ggcccgccgc gatcagcgca gacgacaatg actgatcgcg cgtgacctgc acgagcgcgc
     2221 cgtcgcggag cacgtacgca cgcgaatcac cgacggtggc gatgacgaga cgatcgttga
     2281 cgatgcccgc cgcggacacc gtggtgccca tgcctttgag gccgggctcg cgcttcgaca
     2341 tcgagtgaac atcgtgattc gcgacgcgcg tcgctcgccg caacaagcgc gcgaagactt
     2401 ccggatcgcg cgtgccaggt gtcgcttgca tctcgcgcca gatcgactgc gctgccagat
     2461 ccgatgcgac ctcgccgcct tcgacaccgc ccatgccgtc gcacacgatc atcagcggcc
     2521 cgcgatcggc cttcgccgcg aacggctcgg cgagatccag cttgacgccc ttatcgagat
     2581 taccgacgag gaacgagtcc tcgttgtgct cgcggaccgt gcccacatcc gtggcagccg
     2641 cgacggtgat ctcgagacgg gcgagctcgg tcaacgttcg atccgaagca gctgatcgcc
     2701 cattcgcacg tggtcgccgt tcttgatcgt catcggaccg ctgaggcgga agaacgtgcc
     2761 gttcgagctt ccgagatccg tgagcatcgc gcgcttgccg tcccacgtga tcgcggcgtg
     2821 gcgacgcgac atgaacgcat cgtcgcggaa cacgatatcg ccttcttcac ggccgagcac
     2881 gacctcttca ccgacgagat gacgaacgtc gcgatagccg cccgacggaa tcagctgaag
     2941 gagccggccc catggatcgc gtggcggaga tccgaacagc gcgacgccgt gacgcacgag
     3001 cggatgaatc gtgcgctcct cgggcgtgac gcgctcgaac cgcagcacct cacgaccgac
     3061 gaggacgatc atgccgtcgc tgagctcgac cgatgcgtcc gctttcttga acacgccatt
     3121 cgtcgtgtcg atcggatgga tcttgatcgt gccgtcgggg cccttcgcga tccgcgcgtg
     3181 ctgcttcgcg aggaaccgat cgtcgtcgaa cgcgatgtct gcgcccgcac gaccgacgat
     3241 ggtgtcctcg gacgtgagcg cgaaacgttg gccgtcggtg ccgtcgcgat tgacgaggac
     3301 cgctgcgccc cacgcgccac cggccgtcgc agatgaagga cgcgtcggcg gagcgtttgt
     3361 gccgatggcg actggcggcg gcgccgacgg tgtgtgcgca cgcccaaccg cgatcgcgcc
     3421 taccatcgcg gcggcgcctc cacctccggg cgtcgcgagc gttgcggctt gcggatccac
     3481 gccgggctgg ggcatcggtg ccttgttcat gatgccgagc gctccaccga cgagcggcgg
     3541 gccggacatc ggcggctgat tcgtcggcgg gcggattcgc gagggtgaat tgccagaacc
     3601 agcgccggga tcggtcggtg cgatcggctg catgtgcagg ccgcactgct ggcagtacgc
     3661 aaagctcgtc ggcgtcgcgg tgccgcagcg cgggcacgtg atcgtcgcag ccccaccctg
     3721 cgaaggacgc ccacccgccg acgtcgcgag cgggacgacc aggggcggag acgcattggg
     3781 ccccgtcggt ggcgcgaacg cgggatttgg cggcgggaat ggaaccggcg cggcgatcgg
     3841 ccctcctgga ggtggcgcca tcatcggcgc accgccggga gcaaatgacg gcgagcccgg
     3901 cgtcgtgccg agcgtcgcgc cgcaattgcg acagaacttc attccaggcg ggttctcacc
     3961 tcggcacgcc gtacagatgg tcacgccagg caacccaccc ccaggtacct gagtcgatgc
     4021 agtggcggcg ctgagcgcgg tgccgcagtt caagcagaac gacgaccccg gcgggctctg
     4081 gtggccacaa gcagaacaac gcagcatcac acccctcttg ccccgagttt ggcgaaaagc
     4141 ccggcgcgag tgaatggcac ggggctcatt tgcaacgtgg ggtgcgactt gctaagaccg
     4201 gcccgccgaa tggctcccaa gctcgcaaac gtgctcggaa agttcctcgt cgtcctgttg
     4261 ctcctgcagg ccgcctgcgc ggagacgtcg atccgtacgg cgccatttcg ctcgcggccc
     4321 gacgctgcgg accctggctc gctcgcgggc ccgttcaccg gacgcgtcct cgatgcgacg
     4381 acaaaggcgc cgatcgccgg tgctctcatc tatgcagcgt ggacgctcga gcgcggcagc
     4441 gcgctggtcg agccggcggg atcgcgtgag gtcgtcggtt cgaccgacgc gggcggctac
     4501 tacaagatca atcagctcgg ctcgctgccc cgcggctcgc gcgtcaccga gttcacgctg
     4561 ctggtctaca agcgcggcta cgtcgcgtat cgcagcgatc gccgcttcta tgatctcggc
     4621 ccgcgcatgg acttcgcgca gtcgggcaat caggtgttgc tcgagcgctg gcgcaacgag
     4681 ctctcgcacg cgcgacatct tcggttcgtt ggatcgggca ccgcggtagc ggcgctcacg
     4741 agttgggagc ttgcagatgc gagcgccgag ctcgagggca agcgtccgtc cggtgacgac
     4801 ctgcgtcccg gcagcagcga aggctcgtac gtcgtcgcgg cgcagctgct gacggacaac
     4861 gacatcaagg cgcgcacgaa atacgacggc caattcgaga cgggtccgct ctccgacgag
     4921 ccggacaccg cgacgtacag ctcgcagcac ttcaaggcgc tcggtcgtcc cgagtcgtgg
     4981 gacgtcgcgc tgcgtgtgtg gcgactcgat gccgcgaagg cgcaagagcg ttacgaagag
     5041 ctgctgacgc agctgcccgg cgtgacggag aaagacgaga ttggttcgcg ctcgttcgtc
     5101 gccgtcgaga acgctcaggg caacgagatt cgcggcgtcg gtttcctcga cggaacgcgc
     5161 ggcatcgtcg tcctgctcac gtgtggtgcg agccagtgca cgagcatcga cgacgcagcg
     5221 ttccttggtg cgagcatcta caacaagctc aagcaaatcg tgccgtcgcc gggaggtggc
     5281 cagtgaaaaa agccgccgca cttctgttcg tggcgctgtt cgcgggtgaa gcgcgtgaag
     5341 cacacgcgtg ggaagcggag acgacgcaag ccgggctcgc cgagcaggcc gccctctcct
     5401 ctcgtctcca caagcgtctc gtggtgctcg gcttcgaagg tggactgttc gagccgctga
     5461 cggttccgcc ggccgatgca cccgcgttga tcgagacgct gcatctgctg tcgccgtcgc
     5521 atggctgcgt gcccgatgcg cgtggtcgcc agacggcact cgcttggatc gcagccggtg
     5581 cagcgatcgc cgatgtgccg gcaaagctcg gcgcgaatca cttcttcgat ccgtcgacga
     5641 agcagggttg gcgcaagcca tcgcgcggcg tcctcgctgg tctctccgat gtcgttcgcg
     5701 aagcggtcgg tcgcgcgtcc gtgccggcga agggcgtgcc cgcgatcgat tgggtgacgg
     5761 ataagaacaa tccgctcaac ctcaccggct ttctcgatca gtatgcgaag gccgtgtccg
     5821 cggcgacgcc cggtgagcgt tcgcgtgcga tggcgggtgc gctcgttgcc gcgggcgcga
     5881 tgctgcatac gctcggcgat ctcggtgcgc cgtcgcgcgt gcgtggcgac gaagccgctc
     5941 acttcgagca gctcggcggc ggccccgatg atctcggctc gcgattcgag cgcgtcgcgg
     6001 ctctcgcgtt tggccgcctc ggcatccctg ccccgtcacg cgtgatcacg cgcgagcatc
     6061 tgcgcgatta cttcagcgcc gccgatggca gcggtctcgc tgacatgacg gcgactggct
     6121 acttctcgcc gaacacgctg cccggatcga cgcgcgtcgg tgcgaacacg aagccgaagc
     6181 tcgcgcgccc gctgccggcg ctgccgacga agctgaacct catggcggcg acgcgagagg
     6241 acggcaccac gctgcgcaat ccggcgggcg tgtgtctcgc gcgctatcac gtcgatcacg
     6301 gcgtcgtcac gttctcgctc gacgacgaat gcatgctcga ggaagtcacc gcgattctcc
     6361 ccgaggttgc tgcattcgag acggggctgc tcgacttcct gcttcgcggc gagctcaccg
     6421 tgaccgctgc cgcggatctc gcgatcagtg cgaatggcct tggcgcggga acactcgagc
     6481 tgttcgtcga ggatgcgtcg ggcgttcgca agagcgtcgg caagcaagac gttgctgccg
     6541 gcaaactcga cgtgcacgtt gcgcttcccg ccgatggcac acgagtagtc gcgctgttcc
     6601 gcggcgtcga ttccgcgggc gagccgctcg ttgcgatcgg cgctgcaccg ctcgccgctc
     6661 actagcttca ccgccgcacc cgcgatagag ctacgctcga cgtatggcga agaagcgaga
     6721 ggaaggcgac acgctcgatc gcgtgtttgc actcatcgac gaagcgccac cggggcttca
     6781 tgacctcgac gagccgtctg aggatctcgt tcctggatta ccggctccgc tgatcgagct
     6841 gtacgcgcgg tgcgatggcg cgcgcatctt cgtcgaagcg atcgagattc tcgccgcacg
     6901 cgaggtccac ttcgagcatg gtcgctggaa gttcgcagtc atcgatggcg ccgacgtctg
     6961 gatcgatcac cgcggcaaga tttggcgcac tgaggaatcg atcgaagacg acgtctgcga
     7021 tggcacgcgt ctcgatcgat ggctcgccgg ccagctcgat tcgctcgcgc tcgtgttcga
     7081 cggcgatggt gagtacgccg aagacgtctt cgatgaggac ggcgagatca tggcgatcat
     7141 tcgcgagaag caatatcgcg cgcagctcaa gcgcgacgca gcggcaccgg ggcctcgctg
     7201 gagactcgcg cacgtgctgc tcgagcaagg agccgtcgaa gattcgcgca acgagctcga
     7261 gcaagtcgtc gccgatgatc cggcgttctt ctgggcgtgg ctggatctcg cgcgtgtgag
     7321 cgagcgcctc ggtgaaatcg gcggcgcgat cgatgaagcg cgcatggcgg cggaagctgc
     7381 cgaaggcgtg cagcacgcgc aggccggata tttttggtcg caggtcgcgc ggctcgcggt
     7441 gcgcgcgggc gacgacatgg tccgggcgca agcggcgaca aaagcgtcgc tactcgcgcc
     7501 ggagctcaag cgtgcgcaga tggaaggcgc gcgcgagtgt atcgatgcag gagacaaggc
     7561 gagcgcgaag ggtctgttgg atcttctgcg cgcggtgtgg cctcgcgatc tcgaggtgct
     7621 cgagctctat cgtcgcgtcg aaacgatttg acctacacgg aggcactgtc ggccagccat
     7681 cgacaaagcc gacggttact cggacgtaca ttcgactctt cacttcgcgc acttcctgga
     7741 acggcaatta cacgtagact ggatggtgaa tgcgtcgtgc gttcatcctg gatctttcca
     7801 ctcacgggca ggccgcgaag gacctgtccg taggtggtgt cttcatcgag aacgcgtccg
     7861 tcgatttcga cgacgagtgc cacatcattc tgcgcgcagg cagcgaagac ctgaccgtga
     7921 cggcgcgcgc cgtccagatt ctagagggtg gcgccgggtt tcaaatcgaa ggcatgaatc
     7981 gcgagctgcg cgagcgcatt gcgacgctgg tcgacctcgc gatgcactcc gacctcgatc
     8041 gcaaaaagac gctgccccac tcgctcgcca agctcgaggc ggcgaagcgc tccgccgcgg
     8101 gctcgatccc gccggagact cgcagaagcg cgcgcgttgc cgcgggctcg atctcgccga
     8161 tcctcgggct cgctccgaca cagcaagacg acgctctcgg cgacaagatc agagccgcga
     8221 agctcgcatt ggatcgcgaa aaagaaaccg acgactagag cgcggcttcg agcgagaccg
     8281 tgaagatgtg catcgcgccg cgtccgcgga agtttgactg cggtgtcgtc gtcgagtcgg
     8341 tgacctcggg gatcaagtag aagtagcgca ggtagctcgc gcccaggcgg tagcggcctg
     8401 tcgtccagcg cgcgccgctg tgcagaccga tgtgccggaa cgtcggctgg tcgagcgtca
     8461 ccgtcgcggt cggcgcaggc gtgcgatcga agtgcgagcc ggccatcagc tcgatgccgc
     8521 gcagctggcg aacgtcgtgg acgcggacgc cgcccgagac ctgccacgag tcgttgtagt
     8581 tcttcatcgt ctcgagctcg cgcacgagga agatgccgac gacgtccgtg tgctgcttct
     8641 tgtattggcg atagagccaa tagcggccct caccgccaac ctcgatctgc ggtgtgagat
     8701 cgaagctcgc gccggccatg aaggcccacg gcagcatctg cgtcgtcgat tgcgtaccga
     8761 cgagcgtgtc gcccggggtc tcggcatcgt cgctgtacgt gatctgaacg gggcctctca
     8821 tcgtcgcgtc gacgcggccg gtgagcgtcg cgccccacgt cagccgcgga tgcggacggc
     8881 cgtacgccgc gagcatccac gtcggtgccc agccgctgcc gtcgagcacg agctcggggc
     8941 gactgccgat ggtcttgctg acgtccgtgc cgttgacgat cgggtagacg tcgcgcttgc
     9001 cgtgaatgcg gaggttcatc acgccggccg acgcgccaag cgcgactgac tcggtgacgc
     9061 gatacgagcc ggcgacgacg gcttgcggcg cgacgacgta gccatcgatg aggtgatagt
     9121 gcgtgacgtc gtcgtcgttg aacgcggctc cttgcgcgtt gccgacgtag agcgcgctgc
     9181 cgaggacgag cttgccggga atgatctcgc cggtgagtgc gagcatcggg atgacgccca
     9241 tcgcacgcgc cggccgaacc ggctcgtagt agccgtccat gtcggcgctc acgccgagga
     9301 agcgatcgct ctcggcccag ctgtggagct ggaactcgct acggatcatg gcgagcccgc
     9361 tcgataggta gaggtgccaa cctggctgca agatgagacc ggccgggttg tgataaatgg
     9421 ccgcgccttc gtcgggacga ccgatgacgg cgcccatcgc ggtgcggcga acgccgatgt
     9481 ccgggattcc gaagcctccc gcatgcgctg cagagacgga gagaaggatg gtgaaggcgg
     9541 cgagcgtcgg ggtgcgcacg gcctacgaat agccttgttc gagtttattt tacagtgtaa
     9601 aagttggacg tgctcgattt caagatcgtt ggcggcaccg tggtcgatgg aacggggcgc
//
LOCUS       DATLBZ010000681         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_3721053, whole genome shotgun sequence.
ACCESSION   DATLBZ010000681 DATLBZ010000000
VERSION     DATLBZ010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3721053"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VMZ53_33425"
     CDS             complement(<1..663)
                     /locus_tag="VMZ53_33425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS33467.1"
                     /translation="MKIHEYQGKQLFQKYGVPVPVGIPATTAEEAIAAIPKVQQQGGT
                     EVVVVKAQIHAGGRGKGGGVKVAKTRADAEEAVKKIFGMQLVTPQTGPEGKKVQRLLV
                     EQGLAIEHEYYLGMLLDRATGRVTVMASTEGGMDIEEVAEKHPEKILKQPIDPATGWQ
                     DWQSRTLAKQLGMTGKTAGELGKFMKSLTRCYEEMDCSLLEINPLITTKDGKVLALDA
                     KIN"
     gene            728..1456
                     /locus_tag="VMZ53_33430"
     CDS             728..1456
                     /locus_tag="VMZ53_33430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33468.1"
                     /translation="MRLVLVALVVVVSGCAASETTETNDDGEASAAPAQELGKEDSAA
                     YLGQFRSHATRHYNGDVPALELRADGSYIRARCYHASCALQVAETDHYDTYTSSSGKS
                     YIRFWSFTAVRDADDNLTHDARIADVYEVRTFSHGIQVRKAYSSRWQSLYTSSPSLAC
                     AATGGTWASDACTCAGNDPTDWWPNNIFIAGAGGCMHNPGTSEENCDATNGEWTDDDA
                     TLTGSYCLCGFGRYLDDDGACAEL"
     gene            complement(1453..>2568)
                     /locus_tag="VMZ53_33435"
     CDS             complement(1453..>2568)
                     /locus_tag="VMZ53_33435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33469.1"
                     /translation="HFEAADAPKNAAEVLEVEAEILLMRHDEHALDDAMFAARKAEDA
                     LAKAGLPILPAIDRILGDIAFERRDFSEAHRYYDRDRRQGHRGRNLATIAGKVVGGDG
                     KPVTVMAWYGDMKGDPTRLYTSFEPEAAVQTNADGTFELEVEAGWAIMAESRTMRSRP
                     QTIAPGKSPTLTLAPTTTMSGTVTGKNLQGLRVLARYPLGENGFVVQVPVERDATFHL
                     AGLIAGMTPTLELTTNDVIPDRTVRATKDGQLRWPYGQALDLIVRGSTSGEAVVVRGT
                     VSPKTHKQLEALPHDASCTFSEVARRNTDIGREVYRAGDRHCIVYGVEDGPVSVCADD
                     TLSEKVTCTAVDVKLSVSVEYPDGRFGGGTTPVVVTF"
BASE COUNT          380 a          851 c          839 g          498 t
ORIGIN      
        1 gttgatcttc gcgtcgagcg cgaggacctt gccgtccttc gtcgtgatca gcgggttgat
       61 ctcgaggagc gagcagtcca tctcctcgta gcaacgcgtg agcgacttca tgaacttgcc
      121 gagctcgccg gcggtcttgc ccgtcatgcc gagctgcttc gcgagcgtgc gcgactgcca
      181 gtcttgccag ccggtcgctg gatcgatcgg ctgcttgagg atcttctcgg ggtgcttttc
      241 agcgacctct tcgatgtcca tgccgccttc ggtcgacgcc atcaccgtga cgcggcccgt
      301 ggcgcggtcg aggagcatgc cgaggtagta ctcgtgctcg atcgcgaggc cttgctcgac
      361 gagcaggcgc tggaccttct tgccttcggg gccggtctgc ggcgtgacga gctgcatgcc
      421 gaagatcttc ttgacggcct cttcggcatc cgcgcgagtc ttcgcgacct tcacgccgcc
      481 gcccttgccg cgaccacctg cgtggatctg cgccttgacg acgacgacct cggtgccgcc
      541 ttgttgctgc accttcggga tcgcggcgat cgcttcttcc gcggtcgttg cgggaatgcc
      601 gaccggcacc ggcacgccgt acttctgaaa gagctgcttg ccctggtact cgtggatctt
      661 cacgccgtcg gtgtatcact ttttttggct tgctcgggac cgccgcggcg cgttatgagg
      721 ccgcccaatg cgtctcgttc tcgtcgctct ggtggtcgtg gtcagtgggt gtgcagcgtc
      781 ggaaacgacg gaaacgaatg acgacggcga ggcctcagcg gcgcccgcgc aggagctcgg
      841 caaggaagac agcgccgcct atctgggcca gttccgttcg cacgcgacgc gccactacaa
      901 cggcgacgtg ccggcgctcg agcttcgcgc tgatggctcg tacattcgtg cgcgttgcta
      961 tcacgcgagc tgcgcgttgc aggtcgccga gaccgatcac tacgacacgt acacgagcag
     1021 ctcgggcaag agctacatcc gcttctggtc gttcaccgcc gttcgcgacg ccgatgacaa
     1081 cctcacgcat gacgcgcgga tcgctgacgt gtacgaagtc cggacgttta gccacggcat
     1141 tcaggtccgc aaggcgtact cgtcgcgctg gcaatcgctc tacacgtcga gcccgtcgct
     1201 cgcgtgtgct gcgaccggcg gcacgtgggc gagcgatgcg tgcacgtgcg cgggcaatga
     1261 ccccaccgac tggtggccga ataacatctt catcgcaggt gcgggcggtt gcatgcacaa
     1321 ccccggcacg agcgaagaga actgcgacgc gacgaacggc gagtggaccg acgacgatgc
     1381 gacgctgacc ggctcgtact gcctgtgcgg cttcggccgt tacctcgacg acgacggcgc
     1441 ctgcgcggag ctctagaacg taacgacgac gggcgtggtc ccaccgccga agcggccgtc
     1501 cgggtactcg acggagacgc tgagcttcac gtccacggcc gtgcacgtga ccttctcgga
     1561 cagcgtgtca tccgcgcaga cggagaccgg cccgtcttcg actccataca cgatgcagtg
     1621 gcggtctccc gcccgataga cctcgcgtcc gatgtccgtg tttcgccgcg cgacttcgct
     1681 gaacgtacaa ctcgcgtcgt gcggaagcgc ttcgagctgc ttgtgcgtct tcggcgacac
     1741 ggtgccgcgc acgacgactg cttcgccgct cgtcgagccg cgcacgatca ggtcgagtgc
     1801 ttggccgtac ggccatcgga gttgcccgtc cttcgtcgcg cgcaccgtgc gatcagggat
     1861 gacgtcgttc gtcgtcagct cgagcgtcgg cgtcatgccc gcgatgaggc cggcgagatg
     1921 aaacgtggcg tcgcgctcga cgggcacttg cacgacaaag ccgttctccc cgagcggata
     1981 gcgcgcgagc acccgcagcc cctggagatt cttgcccgtg acggtgccgg acatcgtcgt
     2041 cgttggtgcg agcgtcagtg tcggcgattt gccgggcgcg atcgtctgcg gtcgcgagcg
     2101 catggtccgg ctctccgcca tgatcgccca gccggcttcg acctcgagct cgaacgtccc
     2161 atcggcgttg gtctgcacgg cggcctcggg ctcgaagctc gtgtagaggc gcgtcgggtc
     2221 gcctttcatg tcgccgtacc acgccatgac cgtgaccggc ttgccatcac cgccgacgac
     2281 cttgccggcg atcgttgcga ggtttcggcc gcggtgtcct tggcggcgat cgcgatcgta
     2341 gtagcgatgc gcttcgctga agtcgcggcg ctcgaacgcg atgtcaccga ggatgcgatc
     2401 gatggccggc aagattggca gccccgcctt cgcgagcgcg tcctcggcct tgcgggcggc
     2461 gaacatcgcg tcgtcgagcg cgtgctcgtc atgacgcatc aataggatct ctgcctcgac
     2521 ctcgagaacc tccgctgcgt tcttcggagc gtcggctgct tcgaagtg
//
LOCUS       DATLBZ010000682         7759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6503671, whole genome shotgun sequence.
ACCESSION   DATLBZ010000682 DATLBZ010000000
VERSION     DATLBZ010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6503671"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VMZ53_33440"
     CDS             <1..183
                     /locus_tag="VMZ53_33440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33470.1"
                     /translation="QRVEHLTERVVQNFKLLSDRIDTTTGRIVESEMRVATASVDLAG
                     TVNDIKTILIDRLGRR"
     gene            complement(180..1625)
                     /locus_tag="VMZ53_33445"
     CDS             complement(180..1625)
                     /locus_tag="VMZ53_33445"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUS33471.1"
                     /translation="MTRIAIVGGGAAGLAMGMQLKRAGHDDFVILERSPSVGGTWHDN
                     RYPGAACDVPSHLYCFSFAPKPDWTYKFSRQPEIEAYMRACVEQHGLGPHLRLGTEVR
                     GAKLDGGAWHIETSGGPSTDVVAEVLVSGTGQLNRPRVPALPGLDSFAGHAFHSSRWR
                     PDFAAAGKRIIVVGTGASAVQIVPELVRDAAHVTVMQRSPAYVIPRNDRAYWRLEKWL
                     FRYAPGWRALYRSLIYWSLEARFTALHRSSVMGAITRWMAMRHLRKSIRDASLRERLT
                     PTYPIGCKRIVISDDWYPALARDNCSVVSAPIERIVPEGVVTADGATHAADAIVFATG
                     FDSTSFLAPMQIVGRDGVALHDAWRTGAEAHHGMLVSGFPNLFLLYGPNTNLGHNSIL
                     FMIECQVRYVLRCLDELGRRGATWIDVKREAMTRSNAELQAALAKTTWSSDCNSWYKT
                     ADGKITNNWSGRTAAYWRATRNPTFDELDLG"
     gene            complement(1625..4039)
                     /locus_tag="VMZ53_33450"
     CDS             complement(1625..4039)
                     /locus_tag="VMZ53_33450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33472.1"
                     /translation="MRRVVLCVALLFGGCVQSGLVPCESKACPTDKVCATVDAEPTCV
                     YPEQLSDCTGKADGEPCTIRNTLVSSCIAAICQPVECGNSVRTADEACDDGNNANGDG
                     CSADCTSDESCGNGKTDLAASEQCDDGNTDDSDDCRNTCVLPRCGDGALDPLEECEPA
                     LDVAKACGDFGYYGGTLGCSTSCRFDPAATCVGRCGDAIVQTSNGEQCDGSTARQACL
                     DMGADYGAARCTASCSVANCERFGWTHLVTTGGSFAFTGDNGHLAYVSGGSQVAYETA
                     TGWNMHAATTAYFQFLDQRGDDLLVATDAEVERYLNGTWAKLPALLINPSEPIKAVMF
                     ADDGTPTVLVSTGYTGRVLQYIGSAWTSTSLAVGGSLLDAYAAGKFAVALDNGSVEYH
                     AGGITRSVPITGTVVGLAIHDDVELAVYTAAGTTISETRASITGTSTNLGVRLEGYAG
                     GRIVVADAHVYALDNGKLLRGTGRHTEVITGPTDQSPLSQVLVATRDGRVLVEAANSL
                     WKLSSLAPTLRTLPMANVFDSDLRFGLTTDGAWLLCDTEVWIAGSAFAKISTTPSLRC
                     YGIVGTSKSDIYVERESSSLENRLYHFNGSGWTEELYNGVRIDARLLALDSALYVVLS
                     NGFIPGADTLIKSGGVWAPGPSLPAGCAASALAAGAGQVYISGVCSNTARMWHLSAGA
                     WVEDTDLAQPMSPYGAYSSISIGEDGSVFAGSGRLAAYYGGAWHFFTGLGIGRRVAAI
                     SATDAFAGGGGTGAPIWHFDGTDWSPIRVDPTFSVSWLVATKHDVGVIGMMPSTRSTW
                     VGYSRE"
     gene            complement(4052..5422)
                     /locus_tag="VMZ53_33455"
     CDS             complement(4052..5422)
                     /locus_tag="VMZ53_33455"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HUS33473.1"
                     /translation="MTRGQILDSILDTTGMTPLVRLSRIGRDLPGELLGKCEFMNPGG
                     SVKDRIGVRMLLDAEKAGRIKPGDTLIEPTSGNTGIGLAMAAAVRGYRVIITMPEKMS
                     QEKQVVLEALGAEIIRTPTEAAWDAPESHIGVARRLREIIPNSHILDQYSNPSNPGAH
                     EEGTGREIIDQCGGKLDAVVMTAGTGGTISGVAKVIKREVPGCKVVGVDPEGSILAGP
                     SEIKTYKVEGIGYDFIPDVLDRRLVDQWIKSNDRDSFLVARQLIRQEGLLIGGSSGAA
                     VWAAMQVSRELGPGKRVVVILPDSIRNYLTKFVDDKWMRQQGFFKADWEVGTVGDVCR
                     AVGRRNVISLDLNDKVSRATDLFKQHGISQMPVVDNAKLAGILTESDLLHQLMTGRVD
                     RDTIVAEVMERKVSTVSMHANSGELPRIFERGEVAIVVDEDRAVLGILTKMDLIEMLA
                     ARKTKV"
     gene            5447..6196
                     /locus_tag="VMZ53_33460"
     CDS             5447..6196
                     /locus_tag="VMZ53_33460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33474.1"
                     /translation="MLRVAAVFAAALLALVASCFGKPGAPHATGGDDDSDARMDSGSG
                     SGSGSGSGSQMMCTGVPTDNFETGTECGALGTAYGSAAVSRTNGQLYVQPRGIPGGCR
                     TATAFDFSHGVSIKVVQIASSGMPNDTYVRAYNGADMTQGIEVLFSTLSSSPVVDISC
                     FGASAPVPVELNYNNAAHLWWKIEAATAAQGTTVHAYYSANGTSWTDTSIACTWTSTS
                     TVKLDVGTGASASGANLPAIFDDFNIKTCPP"
     gene            6193..6945
                     /locus_tag="VMZ53_33465"
     CDS             6193..6945
                     /locus_tag="VMZ53_33465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33475.1"
                     /translation="MKRALRVIAVLGAAAIAACFTKPGEPHLTDGGNGTRDAGPDGVG
                     MDGRGDGPDAPPSGCGTQDDFNVASVMDCQDWGDAVVSTGTTITRLNGTLDFNFTGTS
                     SSASCTTKTAFNITNGTSIRIVEPSNYQTSFSLTVSGKTVTYVLTYTGTMYSAVLICP
                     TGGTSNASAPLIAGPPAWIQMKVSVPGAGGLTVALETSPSGNLGTWTQFQTCTFTAAA
                     ATTGIVEMRGSGSGATYTKFDDFNMKNCPPPL"
     gene            complement(6914..>7759)
                     /gene="phnD"
                     /locus_tag="VMZ53_33470"
     CDS             complement(6914..>7759)
                     /gene="phnD"
                     /locus_tag="VMZ53_33470"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HUS33476.1"
                     /translation="VETVVVDTQKPIRFGLSPALGVQAARTQGERVAAWLKTRFDREV
                     RPVVFGDYPGLVDAISEGEVDFAWMPPISFVRAADRGVGVVALASRYGRPTYESAIVV
                     RADSPLVSLADLRGKSIAYVDRESASGYLFAADLIQRELGAPATVLREQHFQGSHKAV
                     TDAVRRKWVEAGVTYVVRNADGRIVYSGWLDLAQASDVPLRVLATTDPIPCDAIAHRP
                     GLPSGIVERLAKTLVEVDDDPMGREVLTQIFHTTGMVRADLRIYDAVRDALARVTAAA
                     GSSSC"
BASE COUNT         1357 a         2588 c         2492 g         1322 t
ORIGIN      
        1 cagcgcgtcg agcatttgac cgaacgcgtg gttcagaact tcaagctcct gagcgatcgg
       61 atcgacacga cgactgggcg catcgtcgag tcggagatgc gcgtggcgac ggcgagcgtc
      121 gatctcgccg gcaccgtaaa cgacatcaag acgatcctca tcgatcgcct tggtcgccgc
      181 tagccgaggt cgagctcgtc gaaggttggg ttgcgcgttg cgcgccagta cgcggcggtg
      241 cggcctgacc agttgttcgt gatcttgccg tcggccgtct tgtaccagct gttgcagtcg
      301 gacgaccacg tcgtcttcgc gagcgcggct tgtagctccg cattcgagcg cgtcatcgct
      361 tcgcgtttca cgtcgatcca tgtcgcgcct cggcgcccga gctcgtcgag gcagcgcagc
      421 acgtagcgga cttggcactc gatcatgaag aggatcgagt tgtggccgag gtttgtgttc
      481 gggccgtaga gcaggaagag gtttgggaag ccgctgacga gcatgccgtg atgcgcttcg
      541 gcgcccgtgc gccacgcgtc gtgcagcgcg acgccgtcgc gaccgacgat ttgcattggc
      601 gcgaggaagc tcgtcgagtc gaagcctgtc gcgaacacga tcgcgtcggc agcgtgggtc
      661 gcgccgtctg ctgttacgac gccctcgggt acgatgcgct cgatcggcgc ggagacgacc
      721 gagcagttgt cgcgcgcgag tgccggatac cagtcgtcgg agatcacgat ccgcttgcag
      781 ccgatcggat aggttggcgt cagccgttcg cgcagcgagg cgtcgcggat cgactttctg
      841 aggtggcgca tcgccatcca tcgcgtgatc gcgcccatca cgctcgagcg atgcagcgcc
      901 gtgaagcgcg cctcgagcga ccagtagatg agtgaccggt agagcgcacg ccagccaggc
      961 gcgtagcgga atagccactt ctcgagtcgc cagtacgcgc ggtcattgcg cgggatcacg
     1021 tacgccggcg accgctgcat caccgtcacg tgggcggcat cgcgcacgag ctccggcacg
     1081 atctgcaccg cgctcgcgcc agtgccgacg acgatgatgc gcttgcccgc cgcggcgaag
     1141 tcgggacgcc agcgcgacga gtggaatgcg tgtcccgcga acgagtcgag gcccggcagc
     1201 gccggcacgc gagggcgatt gagctggccc gtgcccgaga cgagcacctc cgcgacaaca
     1261 tccgtgctcg ggccgccgct cgtctcgatg tgccatgcac cgccatcgag cttcgcgcca
     1321 cgcacttccg tgccgagccg caggtgcggg ccgaggccat gctgctcgac gcacgcacgc
     1381 atgtacgctt cgatttccgg ctggcgcgag aacttgtacg tccagtcggg cttcggcgcg
     1441 aacgagaagc agtagagatg gctcggcaca tcgcacgctg cgcctggata acgattgtcg
     1501 tgccacgtcc cgcccacgct cggcgagcgc tcgaggatca cgaagtcatc gtgaccggcg
     1561 cgcttgagct gcatgcccat tgcgagcccc gctgcgccgc cccccacgat cgcgattcgc
     1621 gtcactactc gcgagagtaa cccacccagg tcgatcgcgt gctgggcatc atgccgatga
     1681 cgccgacatc gtgcttcgtc gcgaccagcc aggacaccga gaacgtcggg tcgacgcgaa
     1741 tgggtgacca gtcggtgccg tcgaaatgcc agatcggtgc gccggtcccg ccaccgcctg
     1801 caaacgcatc cgtcgcgctg atggcggcga cccttcgacc gatgccaagg cccgtgaaga
     1861 agtgccacgc tccgccgtag tacgccgcga gacggcccga accggcgaac acgctgccat
     1921 cttcgccgat cgagatcgac gagtaggcgc cgtacggcga catcggctgc gcgaggtcgg
     1981 tatcttcgac ccacgcaccc gcggacagat gccacatgcg cgcggtgttc gaacaaacac
     2041 cactgatgta gacctgccct gcgcctgccg cgagcgcgga ggccgcgcaa cccgccggca
     2101 gagacggacc cggtgcccac actccgccgg acttgatcag cgtgtccgct ccggggatga
     2161 aaccattcga cagcacgacg tacagcgcgg agtcgagcgc gagcaggcgc gcgtcgatgc
     2221 gcaccccgtt gtagagctcc tccgtccagc cagagccgtt gaagtgatag aggcggttct
     2281 ccaacgagga ggactcgcgt tcgacgtaga tgtcggactt gctcgttcca acgatgccgt
     2341 agcaacgcaa gctgggtgtc gtcgagatct tcgcgaacgc actaccggcg atccacacct
     2401 cggtatcgca aaggagccac gcaccatcgg tggtgagtcc aaagcggagg tcgctgtcga
     2461 acacgttcgc cattggcagc gtgcgcagcg tcggcgcgag ggagctcagt ttccagagcg
     2521 agtttgctgc ttccacgagc acgcgaccgt cgcgcgtcgc gacgaggacc tgcgacaagg
     2581 gcgattggtc ggttggaccc gtgatcactt cggtgtgccg gccagtgcca cgcagcagct
     2641 tcccgttatc gagcgcatag acgtgagcat cggcaacgac gatccgacca cccgcataac
     2701 cttcgaggcg gacaccgagg ttcgtgctcg tgcccgtgat cgatgcgcgt gtctcgctga
     2761 tcgtcgtccc cgctgccgta tagaccgcga gctcgacgtc atcgtggatc gcaagtccga
     2821 caacggtccc ggtgatcggt acgctgcgcg tgattccacc cgcgtggtac tcgacactgc
     2881 cattatcgag ggcgacggcg aacttaccgg ccgcgtatgc gtcgagcaga cttcccccga
     2941 cggccagaga cgtcgatgtc cacgcgctgc cgatatactg gaggacgcgg cccgtatagc
     3001 ccgtcgagac gagcaccgtc ggcgtgccat cgtcggcaaa catcacggcc ttgatcggct
     3061 cgctcggatt gatcaggagc gctggcagct tcgcccaggt cccattgaga taacgctcga
     3121 cttcggcatc ggtcgcgacg aggaggtcgt cgcctcgctg gtcgaggaac tggaagtacg
     3181 cggtggtcgc cgcgtgcatg ttccagcccg tcgcagtctc gtaggcgacc tgcgatccgc
     3241 cactgacata tgcgaggtgt ccgttgtcac cggtgaacgc gaacgaaccg cctgtggtca
     3301 cgaggtgcgt ccagccaaag cgctcgcagt tggcgaccga gcaactcgcc gtgcatcgcg
     3361 ccgcaccata atcggcgccc atatcgaggc acgcttggcg cgccgtcgag ccatcgcatt
     3421 gctcgccgtt cgacgtctga acgatggcgt cgccgcagcg tccaacacac gtcgccgctg
     3481 gatcgaatcg acatgaagtg ctgcagccga gcgtcccgcc gtaataaccg aaatcgccgc
     3541 acgcctttgc gacatcgagc gccggctcgc attcctcgag cggatcgagc gcaccatcgc
     3601 cgcagcgcgg tagcacgcac gtgttgcggc aatcatcgct gtcgtcggtg ttgccgtcgt
     3661 cgcactgctc gctcgccgcg agatccgtct tgccgttgcc acagctctcg tcggacgtgc
     3721 agtcggccga gcaaccatcg ccattcgcgt tgttgccgtc atcgcacgct tcgtccgcgg
     3781 ttcggacact attgccgcac tcgaccggct ggcaaatcgc cgcgatgcac gacgacacaa
     3841 gcgtgttgcg aatcgtgcat ggctcgccgt cggctttccc ggtgcagtcg gagagctgct
     3901 cgggatagac gcacgtcggt tcggcgtcga ccgtcgcgca gaccttgtcg gtcggacacg
     3961 ctttcgattc gcacggcacg aggccgctct gcacgcagcc accaaacagc agagctacgc
     4021 acagaacaac acgtcgcacc tgaccatcat ctcagacctt cgtcttgcgt gcagcgagca
     4081 tctcgatcag atccatcttc gtcagaattc ccaggacggc cctgtcctcg tcgacgacga
     4141 ttgcgacttc gccacgctcg aagatgcgcg gcagctcgcc cgaattcgcg tgcatggaga
     4201 cggtggagac cttgcgctcc atcacctcgg caacgatcgt gtcgcggtcc acgcggccgg
     4261 tcatcagctg atgcagcagg tcgctctccg tgaggatgcc ggcgagcttg gcgttgtcga
     4321 cgaccggcat ctgcgagatg ccgtgctgct tgaatagatc tgtcgcgcgg ctgaccttgt
     4381 cgttgagatc gagcgagatg acgttgcggc gcccgaccgc gcggcacaca tcgccgacgg
     4441 tgccgacttc ccagtccgct ttgaagaagc cctgctgccg catccacttg tcgtcgacga
     4501 acttcgtcag gtagttgcgg atcgagtccg gcaggatcac gacgacgcgc ttgcccgggc
     4561 cgagctcgcg cgacacctgc atcgccgccc acaccgcggc acccgacgag ccgccgatca
     4621 gcaggccctc ttggcggatc agctggcgcg cgacgaggaa cgaatcgcga tcgttgctct
     4681 tgatccattg atcgacgagg cggcgatcga gcacgtcagg aatgaagtcg tagccgatgc
     4741 cctcgacctt gtacgtcttg atctccgacg gccccgcgag gatcgagcct tccggatcga
     4801 caccgacgac tttgcagccc ggcacctcgc gcttgatcac cttcgcgaca ccgctgatcg
     4861 tgccgcccgt gcctgcggtc atgacgaccg cgtcgagctt gccgccacac tgatcgatga
     4921 tctcgcggcc ggtgccctct tcgtgcgcgc ccgggttcga cgggttgctg tactgatcga
     4981 ggatgtgcga gttcggaatg atctcgcgca gccggcgcgc gacgccgatg tggctctccg
     5041 gcgcgtccca cgcagcttcg gtgggtgtgc gaatgatctc agcgccgagt gcctcgagca
     5101 cgacctgctt ttcctgcgac atcttctcgg gcatcgtgat gatcacgcgg tagccgcgaa
     5161 ccgccgctgc catcgcgagg ccgataccgg tgttgccgct cgtgggctcg atcagcgtgt
     5221 cgcccggctt gatgcggccg gctttctcgg cgtcgagcag catgcgcaca ccgatgcggt
     5281 ccttcacgga cccacccggg ttcatgaact cgcacttgcc cagaagctcg cccggcaagt
     5341 ccctgccaat acgtgagagg cgcaccagcg gggtcattcc ggtggtgtcg aggatcgagt
     5401 cgaggatttg gccgcgtgtc atgcgcgggc agtctatgat gaatgcgtgc tccgtgtcgc
     5461 cgccgtgttc gccgctgcgc tcctcgcact ggtcgcgtcg tgcttcggca agcccggcgc
     5521 gcctcacgcg accggaggcg atgacgacag cgatgcgcgg atggattccg gatcaggatc
     5581 tggctcaggc tcggggtcgg gctcgcaaat gatgtgcacg ggcgtgccta cggataattt
     5641 cgagacggga acggaatgcg gtgcgctcgg cactgcgtat ggctcggcag ccgtcagccg
     5701 cacgaacggt cagctctacg tgcagccgcg cgggattccc ggaggctgtc gcacggccac
     5761 ggcgttcgac ttttcgcacg gcgtctcgat caaggtcgtg cagatcgcgt cgagcggaat
     5821 gccgaacgat acatacgttc gcgcatacaa cggtgccgac atgacgcaag gcatcgaggt
     5881 cttgttctcg accctgtcga gctcgccggt cgtcgacatc tcgtgcttcg gcgcgtccgc
     5941 accggtgccc gtcgagctca attacaacaa cgcagcgcac ctgtggtgga agatcgaagc
     6001 agcgactgcg gcacagggga cgaccgtcca cgcgtactac agcgcgaacg gcacgagttg
     6061 gacggatacg tcgatcgcgt gcacgtggac atcgacctcg accgtgaagc tcgacgtcgg
     6121 caccggcgca tccgcctcgg gtgcgaacct gccggcgatc ttcgacgact tcaacatcaa
     6181 gacgtgcccg ccgtgaagcg cgcgttgcgt gtgatcgcag tcctcggtgc tgccgcgatc
     6241 gcggcgtgct tcacaaaacc tggcgagcct cacctcaccg acggaggcaa cggcacgcgc
     6301 gacgcaggcc ccgacggtgt cggcatggac ggcaggggcg acgggcccga cgcaccgccg
     6361 agtggctgcg gtacgcagga cgacttcaac gtcgcgtccg tcatggactg tcaagactgg
     6421 ggcgatgcgg tcgtgtcgac gggcacgacc atcacgcggc tcaacggcac tctcgacttc
     6481 aacttcaccg gcacgtcgtc gagcgcatcg tgtacgacga agacggcgtt caacatcacg
     6541 aacgggacgt cgatccgaat cgtcgaaccg tcgaactacc agacgtcgtt ctcgctgacg
     6601 gtctcgggca agacggtgac ctacgtgctg acctacaccg gaacgatgta ctccgcggtc
     6661 ctcatctgcc cgacgggcgg cacatcgaac gcctcggcac cactcattgc gggaccaccc
     6721 gcgtggatcc agatgaaggt gagcgttcca ggtgcgggcg ggctcaccgt cgcgctcgaa
     6781 acctcgccga gtggaaatct gggaacgtgg acgcagttcc agacctgcac gttcaccgcg
     6841 gcggcagcca cgacggggat cgtcgagatg cgcggcagcg gctcgggcgc gacctacacc
     6901 aagttcgacg acttcaacat gaagaactgc ccgccgccgc tgtaacgcgc gcgagcgcgt
     6961 cgcgcacggc gtcgtagatg cgcaggtccg cgcggaccat gccggtcgtg tggaagatct
     7021 gcgtcagcac ctcgcggccc atcggatcgt catcgacttc gacgagcgtc ttcgcgaggc
     7081 gctcgacgat tcccgacgga agccccggcc gatgcgcgat cgcatcgcac ggaatgggat
     7141 cggtcgtcgc gagcacgcgc agcggaacgt cagaggcctg cgcgagatcg agccagcccg
     7201 agtagacgat ccgaccgtcc gcgttccgga cgacatacgt cacgcccgcc tctacccatt
     7261 tgcgacgaac cgcatccgtg actgctttgt gcgagccctg gaagtgctgc tcgcggagga
     7321 cggtcgccgg cgcgccgagc tcgcgctgga tgagatcggc cgcgaacagg tagccactcg
     7381 ccgattcgcg atcgacgtat gcgatcgact tgccgcgcag atctgcgagg ctcacgagcg
     7441 gcgaatctgc acgcacgacg atcgcggact cgtacgtcgg ccggccgtag cgagacgcga
     7501 gcgcgacgac accgacgccc cgatcggccg cgcgcacaaa cgagatcggc ggcatccacg
     7561 cgaagtcgac ctcgccttcg ctgatcgcat cgacgagccc ggggtagtcg ccgaacacga
     7621 ccggccgcac ctcgcgatcg aagcgcgtct tcagccacgc cgcgactcgc tcgccttgcg
     7681 tgcgtgcggc ctgcacgccg agcgcgggcg acagcccgaa tcggatcggc ttctgcgtat
     7741 cgacgacaac cgtctcgac
//
LOCUS       DATLBZ010000683         6956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_7435938, whole genome shotgun sequence.
ACCESSION   DATLBZ010000683 DATLBZ010000000
VERSION     DATLBZ010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7435938"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="VMZ53_33475"
     CDS             <1..1554
                     /locus_tag="VMZ53_33475"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HUS33477.1"
                     /translation="RRSVMARRETVQRSEAADTGEAQADRGDATRATDSGFTSKGAIP
                     PVESGFASAKPSASERAFGAGHDEDLWAKGDDLDAKDDAERRRDQRFDNDALLEEIAA
                     GTKVIKTGDKGRAVTKLQVALLDLGYLKGGQVDGDFGGETETALKKFQADAGVGPTGE
                     FDAATLDGLRTRFGNRKPYLARSKHKAEGVHRLEDFEKAEAIEALVPRKPGDKFNEEV
                     AGEKYGPKVEQRLAEKIAKYHAQFAVNSAKRDNPKGNFFSWSALEGPCQAAKTATDKV
                     YGSYKTMPAFDHKKGTLKDYFEDQKAANKGLTPDEKKEKAAGLVRYMINSQCADINKV
                     HHADVDGSAELAILDPIVEALVSSEDKVQIILDLLMDWPGAARGGKMFLQRYKADTDD
                     KNREQLWGLFHTGIHEYIHTLKHDTYQEWANDLGGARYHTLIEGFCDFFTLNVRSQLA
                     IDATLQKHVEGAFYDKDKSPPANADGSTGVGVYRSHAQAEQVVSIVGVRNAQEAYFCG
                     DVAKMGAGS"
     gene            1551..2216
                     /locus_tag="VMZ53_33480"
     CDS             1551..2216
                     /locus_tag="VMZ53_33480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33478.1"
                     /translation="MKWLAAIALLAAACSPNDSKPRRPDPPREASTTSPPTTDAQPSQ
                     PTAIDAATGATVTTPKTCAEAKQAIEARRFVGWRGLPANCSPDEMFGVKFDEKEWPAR
                     MLGKHSTRWLEVELTGYYEPTVSVRDNKVVMFDGMNPELAGGWAPLAADLGEPEAKLD
                     WHRDTLLMKGGEWVYPARGITIFGRAAGNDALHIAVYSPTTLADYKASLRPDLGTKLQ
                     PRP"
     gene            complement(2234..3244)
                     /locus_tag="VMZ53_33485"
     CDS             complement(2234..3244)
                     /locus_tag="VMZ53_33485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS33479.1"
                     /translation="MVRSKAVTEESDEVIERARQRARLVLDLAREREDHGLVRSGVAG
                     GSRETVAQERRTEDAALAAERGVADAQRMDERERRRAAMIQLLTLERAATDRTLAAER
                     THADRSITAREDLLALVAHDLRNMLSTIMVNASVIVLATDLKTMRGPAVQVQRAGGQM
                     AQLLEDLLDFSSFEAGKLSLASVDVDVVQVVRDAVEIHAEAAKVQKLELTLHADVPKM
                     IVRGDGRRLTRLLMNLIGNALKFTPEDGRVDVHVAVVGDECEIAVTDNGVGIPKEELD
                     SIFERFQRGGIEERQRFSGVGLGLFIARLIADAHGGRVWAESGEAGTGSTFRVRLPIA
                     AP"
     gene            complement(3485..3907)
                     /locus_tag="VMZ53_33490"
     CDS             complement(3485..3907)
                     /locus_tag="VMZ53_33490"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUS33480.1"
                     /translation="MPPKLIETLHVQHDEIKRLAAQVAAAADGGDIRAVCTGVDALGV
                     ALLAHLALEDAHLYPALTTAAERTQLEVPARIARTYEHNMATISVALKAFIEKYSRDF
                     SMADFRRDWVLVSQLLGDRIESEEATLYPLYTSWVEPK"
     gene            4115..5167
                     /locus_tag="VMZ53_33495"
     CDS             4115..5167
                     /locus_tag="VMZ53_33495"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HUS33481.1"
                     /translation="MFASARYEYEALLHALAESCNPGVIVGSSSAGEFTGAQRGEGAA
                     CALAISSPTMRFAAAVGTGVSRDRVAAAREVVASFHGVTTHDYPFRTALVMTDALAGH
                     ADDLIEQLMVATSGAYEFAGGGAGDDAKFSKTHVFCGTRVYTDAVVALEMLSQKPLGV
                     GVQHGWTPASEPYRVTSVTGARLITLDGYPAAEVFESHAEATGQRLDRNDPLSFFLHN
                     IVGIETSSGHRLRVPLAIGADGSVTCAAEIPEGATIRIMKTSSASASDAGASAAEAAV
                     RALGGATPAAALFFDCVATRLRLGDVFGFELEAVQKALGTSALVGCNTYGQIARADGQ
                     FGGFHNCTAVVLAIPM"
     gene            5171..6766
                     /locus_tag="VMZ53_33500"
     CDS             5171..6766
                     /locus_tag="VMZ53_33500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33482.1"
                     /translation="MDLLATIALIDALGRAGDRLETAQRLAKHFGGKALFVLVPDPDR
                     PNTWIPAHGFRGIPSMRGWRELLSRCVNPGTEAGAVAYPDARELDDAIAYVFDGILFV
                     LVGAPATHRDEVRRHLGFNTRVLGAMLRDEAAVRAMRGQLEIERLNAERTTSLAKALD
                     RARSDAQHAIRVKDEFLAMLGHELRNPLAPIVTALQLLRMENANSRAQAVIERHVSHM
                     VRLVDDLLDMSRINSDKIELRKQPLEVGPVITRAVEMVRPLIEQRRNRLVVEVPDRGL
                     VVDGDPARLAQVISNLVTNAAKYSDAGTRIEVRAERVHDRVRISVVDEGIGIEASYLE
                     NVFEQFVQTPQGLDRAAGGLGLGLAIVRSIVERHGGTVRAASEGVGTGSTFVVELPIC
                     HAVPVDSQTDDAPPASLSKGTGAHILVVDDNKDAAAMLAEVLSAYGHRVDVAHDAHDA
                     LSRLEQVTPDAALLDIGLPDMDGYALAQAVRSRLPAIKLIALTGYGQANDRERSRAAG
                     FDAHLVKPVTIANVTRTLEDLLR"
BASE COUNT         1326 a         2301 c         2174 g         1155 t
ORIGIN      
        1 aggaggagcg ttatggcgcg tcgcgagact gttcagcgga gtgaagctgc cgacacgggc
       61 gaggcgcaag cagatcgcgg cgacgcgacg cgcgcaaccg atagcggctt cacgtcgaag
      121 ggcgccattc cgccggtcga gtcgggtttc gcgagcgcga agccatcggc gtccgagcgc
      181 gcattcggcg ccggccatga cgaagatctc tgggccaaag gtgacgacct cgacgccaaa
      241 gacgacgccg agcggcgccg tgatcagcgc ttcgacaacg acgctctgct cgaagagatc
      301 gcagcgggca cgaaagtcat caagaccggc gacaagggcc gcgcggtaac gaagctccaa
      361 gtcgcgttac tcgatctcgg ctacctcaaa ggcgggcagg tcgacggcga cttcggcggc
      421 gagaccgaaa cggcgctgaa gaagttccaa gccgacgcag gagtcggccc gaccggcgaa
      481 ttcgacgccg cgacgctcga cggtctgcgc acgcgcttcg gcaatcgcaa accatacctc
      541 gcgcggtcga agcacaaggc agaaggcgtc catcggctcg aggacttcga gaaggccgaa
      601 gcgatcgaag cgctcgtgcc gcgcaaaccc ggcgacaagt tcaacgaaga agtcgcgggc
      661 gaaaagtacg gacccaaagt cgagcagcgc ctcgccgaaa agatcgcgaa gtaccacgcg
      721 cagtttgccg tcaattccgc gaagcgcgac aacccgaagg gaaacttctt cagctggagc
      781 gcgctcgaag gtccctgcca agccgcgaag accgcgaccg acaaggtgta cggcagctac
      841 aagacgatgc ccgcgttcga ccacaagaag ggcacgctca aggactactt cgaggatcag
      901 aaggccgcga acaaaggcct cacgcccgac gagaagaagg agaaggccgc gggtcttgtc
      961 cgctacatga tcaattcgca gtgcgccgac atcaacaagg tgcaccacgc ggacgtcgac
     1021 ggttccgccg agctcgcgat tctcgatcca atcgtcgagg cgctcgtcag cagtgaagac
     1081 aaagtccaaa tcatcctcga cctactgatg gactggcccg gtgcagcgcg cggaggcaag
     1141 atgttcctgc agcgctacaa agccgatacc gacgacaaga atcgcgagca gctctggggc
     1201 ctcttccaca ccggcatcca cgagtacatc cacacgctca agcacgacac atatcaggaa
     1261 tgggcgaacg acctcggcgg cgcgcgttac cacacgctga tcgaaggctt ctgcgatttc
     1321 ttcacgctca acgtccgcag ccagctcgcg atcgacgcca cgctccagaa gcacgtcgaa
     1381 ggcgccttct acgataaaga caagtcgccg cccgccaacg cggacggttc caccggcgtc
     1441 ggcgtctacc gttcacacgc gcaagcggag caagtcgtgt cgatcgtcgg cgttcgcaac
     1501 gcgcaagagg cgtacttctg cggcgacgtt gccaagatgg gcgccggctc gtgaagtggc
     1561 tcgcggcaat cgcgctgctg gcggcggcgt gctcgccgaa cgactcgaag ccacgccgcc
     1621 ccgatccgcc acgtgaagcc agcaccacgt caccacccac caccgacgcg cagccgagcc
     1681 aaccgaccgc gattgatgcc gcgacaggag ccaccgtgac cacacccaag acgtgcgcgg
     1741 aagcgaagca agcgatcgaa gcgcgacgct tcgtcggctg gcgcggcctg ccggcaaact
     1801 gctctcccga cgaaatgttc ggcgtgaagt tcgacgagaa agagtggccc gcgcgcatgc
     1861 tcggcaagca cagcacgcgc tggctcgagg tcgagctcac cggctactac gaacccacgg
     1921 tcagcgtgcg cgacaacaag gtcgtcatgt tcgacggcat gaaccccgag ctcgcaggtg
     1981 gctgggcgcc gctcgccgct gacctcggtg agcccgaggc gaagctcgat tggcaccgcg
     2041 acacgctgct gatgaagggc ggcgagtggg tctaccccgc gcgcgggatc acgatcttcg
     2101 gccgcgccgc aggtaacgac gcgctccaca tcgcggtcta ttcaccgacg acgctcgccg
     2161 actacaaagc aagcctacgc cctgatctcg gcacgaagct gcagccacga ccgtagctcg
     2221 tcgtcgtcgc gactcaggga gctgcgatcg gcaaccgcac gcggaacgtc gacccggtac
     2281 ctgcctcgcc gctctccgcc cacacgcggc cgccatgcgc atccgcgatc aggcgcgcga
     2341 tgaacaggcc aagcccaacg cccgaaaacc gctgccgctc ttcgatgccg ccccgctgaa
     2401 agcgctcgaa gatcgaatcg agctcttcct tcggaatgcc gacgccgttg tccgtaacag
     2461 caatctcgca ttcgtcgccg accaccgcga cgtgaacgtc gacgcggcca tcctccggcg
     2521 tgaacttcaa cgcattgccg atcaagttca tcagcaagcg cgtgaggcgt cgaccatcgc
     2581 cgcggacgat catcttcggc acgtccgcat gcagtgtcag ctcgagcttc tgcaccttcg
     2641 ccgcttccgc gtggatctcc acggcatcgc gaactacttg gaccacgtcg acgtcgacac
     2701 tcgcgagcga cagcttgccg gcctcgaagc tcgagaaatc gagcaggtct tcgagcagct
     2761 gtgccatctg ccctccggcg cgctgcactt gtactgccgg cccgcgcatc gtcttgaggt
     2821 ctgtcgcgag cacgatgacc gacgcgttga ccatgatcgt gctgagcatg ttgcgcaggt
     2881 catgcgctac gagcgcgagc aagtcctctc gcgcggtgat gcttcgatcg gcgtgcgtgc
     2941 gctctgcggc gagcgtgcga tccgtggccg cgcgctccaa cgtcagcaac tggatcatcg
     3001 ccgcgcgccg gcgttcgcgc tcatccatgc gctgtgcgtc cgcgacgccg cgttccgcag
     3061 ctaacgccgc gtcctcggtc cgccgctctt gcgcgacagt ctcgcgcgac ccgccggcta
     3121 cgccgctacg cacgaggccg tgatcttcac gttcgcgcgc gaggtcgagg acgagtcgtg
     3181 cccgttgccg cgcgcgctcg atgacctcgt cggattcttc cgtgaccgcc ttcgaacgaa
     3241 ccacgtgctc gtgatccgaa cgatcgcgct cggcgcggag gttctgatct gtctccgtcc
     3301 gggaattatc ggtttcgttt gacgacgcca tgccgacctg cacatcggac cctaacacca
     3361 ttgcgagcca gcgcgatcca gttgcgtgcg cgcagaccgc ggcccctccc gctctctagg
     3421 ccacggcatc gacgtggctg cgagagatca cagtgatgat ccgtcgagcc gggggcgtgc
     3481 tcggctattt cggttcgacc cacgacgtgt acagcgggta cagcgttgcc tcttcgcttt
     3541 cgatccggtc gccaagaagc tgcgagacga gaacccagtc gcgccggaag tcggccatcg
     3601 agaagtcgcg cgaatacttc tcgatgaaag ccttcaacgc gacgctgatc gtggccatgt
     3661 tgtgctcgta cgtgcgggcg atacgagccg gaacttccaa ttgcgtgcgc tcggctgccg
     3721 tcgtgagggc tggatacaaa tgcgcgtcct cgagagcgag atgcgcgaga agtgcgacac
     3781 cgagtgcgtc gaccccggtg cacacggcac ggatgtcacc tccgtcggcg gcggccgcga
     3841 cctgggcggc caagcgcttg atctcgtcgt gctggacgtg aagtgtttcg atcaacttcg
     3901 gaggcacacc caaactatcg cacgtgacgg cgtttgcgtc gtcgcgtagt taaaccgggc
     3961 tcgcccgagg cccagacttg ctgtaataga gacctcgatg gcaacgaaaa cggcagcggt
     4021 ttttactgag gcgaccgaca gcagcgtggc cgggcaggag ctcgggcaac aaattcggag
     4081 cagtctcgaa ggtcagcggc cggacgctgt cgtggtgttc gcatccgccc gttacgaata
     4141 cgaagcactc ctgcatgcgc tagccgaatc gtgcaatccc ggcgtcattg tcggatcgtc
     4201 gtcggctggc gaattcaccg gcgcgcaacg cggcgaaggg gccgcctgtg cgctcgcgat
     4261 cagctcgccg accatgcgct tcgccgccgc cgtcggcacc ggggtgagtc gtgatcgtgt
     4321 cgccgcagcg cgtgaggtcg tcgcgagctt ccacggagtc acgacacacg actatccatt
     4381 ccgtacggct ctcgtcatga cggatgcgct ggcggggcat gcggacgatc tgatcgagca
     4441 gctgatggtc gcgacatcgg gtgcctacga gttcgccggt ggcggtgctg gcgatgacgc
     4501 aaagttctcg aagacccacg tgttctgcgg gacgcgcgtt tacaccgatg cagtcgtcgc
     4561 cctcgagatg ctgtcccaaa agccgctggg cgtgggcgtg caacacgggt ggacgcccgc
     4621 gagcgagccc tatcgcgtca ccagtgtgac gggcgcgcgt ctcattactc tcgacggcta
     4681 tccagccgcg gaagtcttcg aatcccatgc tgaggcaacg ggccagcgcc tagatcgcaa
     4741 cgatccgctt tcgttcttcc tccacaacat cgtcggcatc gagacttcgt cgggccaccg
     4801 gttgcgtgta cctctcgcga tcggcgctga cggatcggtc acctgcgcgg cggaaattcc
     4861 agagggagcg accatcagga tcatgaagac gtcgtctgca tcggcttccg acgccggggc
     4921 atcggccgct gaagccgcgg ttcgggccct cggtggagct acgcctgccg cagcactatt
     4981 cttcgattgc gtcgcgacac gactccgcct cggagatgtg ttcggcttcg agctggaggc
     5041 cgttcaaaag gcactcggca cgagcgctct cgtcgggtgc aatacgtacg gccagatcgc
     5101 gcgcgccgat ggccagttcg gcggctttca taactgcacc gcggtcgtcc tcgcgatacc
     5161 aatgtagccg atggaccttc tggcaaccat cgcgctgatc gatgctctcg gccgagcggg
     5221 agatcgactc gagactgcac agcggctcgc gaagcacttc gggggcaagg cgctgttcgt
     5281 cctcgtaccg gatccagatc gtccgaatac gtggatccca gcgcatgggt tccgcggcat
     5341 tccatcgatg cgcggctggc gcgagctcct ctcgcgatgt gtcaaccctg gaacggaggc
     5401 tggggccgtc gcgtatcccg acgcacgcga gctggacgac gcgatcgcgt atgtcttcga
     5461 cggcatcctg ttcgtgctcg ttggtgcgcc cgctacccat cgtgatgaag tgcggcgtca
     5521 cctcggattc aacacgcggg tgctgggcgc gatgctgcgc gacgaggctg cggttcgtgc
     5581 gatgcgcggt cagctcgaga tcgaacgctt gaatgccgaa cggacgacgt cgctcgcaaa
     5641 ggcactcgat cgtgcgcgaa gcgacgcgca gcacgcgatc cgagtcaagg atgagttcct
     5701 cgcgatgctc gggcacgagc tacgaaaccc gctcgcaccc atcgtcacgg ccctccagct
     5761 gctgcgcatg gaaaatgcca acagccgagc gcaagccgtc atcgaacgtc acgtgtctca
     5821 catggtccgg ctcgtcgatg accttctcga catgtctcgc atcaacagcg acaagatcga
     5881 gcttcgcaag cagcctctcg aggtcggtcc cgtgatcacg cgtgcggtcg agatggtgcg
     5941 cccgctgatc gagcaacgtc gcaatcgcct cgtcgtcgag gttcccgacc gtgggctcgt
     6001 ggtcgacggc gatcccgcac ggttggcgca ggtcatctcc aaccttgtca cgaacgcggc
     6061 gaagtactct gacgcgggca cacgcatcga ggtacgtgcc gagcgggtcc atgatcgcgt
     6121 ccggatctcc gtggttgatg aagggatcgg aatcgaagcc tcttacctcg agaacgtgtt
     6181 cgagcagttc gtgcagaccc cgcagggttt ggaccgcgca gccggtggtc tcggcctcgg
     6241 actcgccatc gttcggagca tcgtcgaacg tcacggagga accgtgcgtg cggcgagcga
     6301 aggcgtgggc actgggagca cgttcgtcgt cgagctgccg atctgccacg cagtgcctgt
     6361 cgactcacaa accgacgacg ctccgcccgc gagcctgtca aaaggaacgg gggctcacat
     6421 cctggtcgtc gacgacaaca aagatgcagc agcgatgttg gcggaggtgc tgtcggcgta
     6481 cggtcatcgc gtcgacgtgg cccacgatgc ccacgatgca ctgagccgcc tcgaacaagt
     6541 gacgcccgac gcagcgctac tggacatcgg cctgccggac atggacggct acgcgctcgc
     6601 acaggccgtg cgctcgcgcc tcccggccat aaagctcatc gcgctcaccg gctatggcca
     6661 agcgaatgac cgagagcgct cgcgtgccgc cggcttcgat gcgcatctcg ttaaaccggt
     6721 cacgatcgca aatgtgacgc ggaccctcga agacctcctc aggtagcacg cccgtccgcc
     6781 tcggctgcgc gacccggttg cgttgacaaa taaaaccaag tggtatacta aaccatatgg
     6841 tttcagttga ccggtcgccg cggctcgacc tcgtcttcca cgcgctggcg catccagccc
     6901 ggcgcgcgat cgttcgtcag ctctccatgg gcgagcgcaa cctcagcgag ctcgcg
//
LOCUS       DATLBZ010000684        19404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8935482, whole genome shotgun sequence.
ACCESSION   DATLBZ010000684 DATLBZ010000000
VERSION     DATLBZ010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 19404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19404
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8935482"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VMZ53_33505"
     CDS             <1..1106
                     /locus_tag="VMZ53_33505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33483.1"
                     /translation="LLSAVPPLPIAGGVTLDQLTASLVGPITAVIPSGSVDIQVHAPL
                     SDPKPAQTIVEHCTDIGNFFELAKAQTPGACRIVLQGTTQLELDIWVDNNELRLGAHK
                     GAAPQGKRGGMTSTGSALAASDWTAAFWGRGTMLNLQGIAPATADVPAEVALGIHAIA
                     LINELGAAAKVDPTGVTFRAYLRTAWTNPPGVLAKIAAITGNEIVTGKATEPAAAIAT
                     ATPSAPFAADFAAGQGGLMIPAAAIGVAAAVVIPAILQAVSNTRGDAQAANAPPVDEP
                     APMKNEDLAALLTRAYVEEAWPQWKTEHPDKPCPDKLEDLTKYFGENPGVPVLEDGWG
                     HPLRMTCDPKFGLVVMSFGPDGTFGTPDDIHAP"
     gene            complement(1117..1470)
                     /locus_tag="VMZ53_33510"
     CDS             complement(1117..1470)
                     /locus_tag="VMZ53_33510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33484.1"
                     /translation="MANAILFSWSRSVPGREMLSAEHFTQFVGYLTSLKSNGTINSFE
                     PVFANPSPGSTVNGFFLIQGDIQKLHALSETAEWIEHMTRASMHLENLGFNFASTGSE
                     IMSRMQVWTKSIPAK"
     gene            complement(1595..2227)
                     /locus_tag="VMZ53_33515"
     CDS             complement(1595..2227)
                     /locus_tag="VMZ53_33515"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HUS33485.1"
                     /translation="MRRSPSATAIDDIDDHLFLEGQRIVLEGISYDDYVTMNDLFVDR
                     PGVRFAYLDGTLEIMSPGFRHGGLKTLLARLLELYALESGIPIYGYGQMTKRKKRKKV
                     GVEPDECYYVGASHRRYAQRGRAPDLAIEIVHSHGMRKIEIYRRLGVREVWVIDKGGL
                     TINALWGERYIVRPKSRLFPDLDVAVLMEYAQLEDQDDAVRAFWALLRRA"
     gene            complement(2269..2580)
                     /locus_tag="VMZ53_33520"
     CDS             complement(2269..2580)
                     /locus_tag="VMZ53_33520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33486.1"
                     /translation="MIAQAAAAKLLDDLLYSRVIECVNMGKLMMVELDLWVALCEQSA
                     GNELGSREEAEARDEARLMIERALMRLASARDRYAELQPFGMECADCEEEIAEPRRRR
                     D"
     gene            complement(2577..3638)
                     /gene="recA"
                     /locus_tag="VMZ53_33525"
     CDS             complement(2577..3638)
                     /gene="recA"
                     /locus_tag="VMZ53_33525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HUS33487.1"
                     /translation="MSAQEKKNKSISEAIRAIHKQFGSGSIMRLDGGNEVAAVEVIPT
                     GSVALDVALGCGGLPRGRIVEIYGPESSGKTTLTLHAIAEAQRAGGVCAFIDAEHALD
                     TDYARRLGVDLEDLLVSQPDCGEQALEICDTLVRTGAIDLIVVDSVAALTPRAEIEGD
                     MGDSHMGLQARLMSQALRKLTAVISRTRTCVIFINQLRQKIGVVYGNPETTTGGNALK
                     FYCSVRLDIRRKKAIKRGDDTIGSEVKVKVVKNKLAPPFREAEFEILYGTGVNKLGEL
                     VDTAEKHGLVEKSGTWYSYGGAKIGQGKEKAMAHFEDNPQMQAQLRAALIAQARRAAS
                     GQPPAAPNGHTPPAAGGAS"
     gene            complement(3762..5312)
                     /locus_tag="VMZ53_33530"
     CDS             complement(3762..5312)
                     /locus_tag="VMZ53_33530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33488.1"
                     /translation="MRQLALALLVAASGCNQVFDLKPTIPYDAAPDSPDAPFEVEDRL
                     LWGIAATNTTGAPIAPIYKAIGSESARDMVPTIFVGPPLADGPLMAANYDITDGSFQI
                     PYNLREAPHRIVYTLPNETVPHEIQWSIAGAVIVIPRTTRFDAPNIPAGAGYTITPTG
                     LSGSLTSPSIATTGVFTQQDRTGPFPRSGATTTYDFAANADMMTTPRGAPQGSLGDYV
                     ILGEWVAHTGYESLGGFALTHIDLAAGTQSPPQTQPAWMSGSAVNRNLGTALACPDCF
                     PNIDVTTANNRFDALGSIGAGTRTQTMSYGISPTTKLPAFVPGTSLQNFLAQPLIIPF
                     LTAPSIVTSMTLADPTSMLPFDRVVTVQFATKRTVSGADLYTAMEVMTNVFTGQVEVK
                     APLARQIKLGTNDLSTADGLMINPSSQATALTWADEPSVGNGDDYIVTLYEITANRLA
                     PIRIYHVLTPSVTVDGSLLSPSHQYVFSIQARSGLSKAKQGNYGADTVTFPFTQATTF
                     TAAFNVKP"
     gene            5372..6151
                     /locus_tag="VMZ53_33535"
     CDS             5372..6151
                     /locus_tag="VMZ53_33535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HUS33489.1"
                     /translation="MKLDARLNLFITLVAVFMVCLVVGDIIGGKLTSFHLFGREWIFS
                     VGQIAFPVTFILTDILNEFYGRKVVRRITFLAFFMVGLTFCIIFIAGKLPWAPFTRDP
                     SWKGVNPSQFEVVFTQASDIQIASMFAFLTGNMVDISVFFLLKRATGSRFLWLRATGS
                     TAVSQLIDTVIISGIIWYGVHFQLGWPPVFIDAPKVTFAQFVTIVMTSYLIKLVAAIG
                     VTPIIYGLHELIEKKFGIHPAPAEVESADLEGRDGVQSDSK"
     gene            complement(6133..7425)
                     /locus_tag="VMZ53_33540"
     CDS             complement(6133..7425)
                     /locus_tag="VMZ53_33540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33490.1"
                     /translation="MKRAVLALSLVACKADLHQTVDAQPLDTAIDSLSSETGEAPPNA
                     VKLTVTRVGAPVADVAVFFQAADSSLIAQKLTNASGVAWAIMPNGGFVTAREHKGQQV
                     EELSTFAGVQPADSLELTFSPTGDTTQAMVHVQVPALSNAVAYNVYSPCGEAPMAMAT
                     DDIRLVGCGAVTDLVIVPADVDGNPLGSLFAPNVSVDGPVDLTSSPYQPLRSVQLRYT
                     NVPDFVSFIGMYESLSATRRAFAVTAGEAPVAHAATATVSMPTTSATELTATNLYPTS
                     GEIGIQTIYNWHAATTTYDLDLDTVLLPPFATAPVFAQPTHTLSWTERPGDIQPDVVR
                     ARIHVYRDAIPTGTAWTWRIVAARSGTSVTYPQLPIVDNFDFTPAAGDTVAVEELQTI
                     SLPGGFAGFRTRGFAEARDAIAGTLGRIVQQDLYFESL"
     gene            complement(7422..8186)
                     /locus_tag="VMZ53_33545"
     CDS             complement(7422..8186)
                     /locus_tag="VMZ53_33545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33491.1"
                     /translation="MSARSSVLLLLLGGCDLAFGVGGEVEPCELQAFDSARATDIIPA
                     FRFSVDWDQRFAVAVLDDGSHAELELPSGTPRKVDLGPYITLNAALSPEGDALLYTSS
                     TEPPVLEGALRGAPGSWTLEARTPIATFVGTPTADVFGPRRVLARMHMNDATVQELED
                     DNGTWVPVGGAHDFDSEMPPNLTPNGLTAVFTRRTLTESGDALEVYGMTRPSMDEWFG
                     TPVKILANGNDEAPQLLGHCERLYTIHDGLMRRYDR"
     gene            complement(8170..9150)
                     /locus_tag="VMZ53_33550"
     CDS             complement(8170..9150)
                     /locus_tag="VMZ53_33550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33492.1"
                     /translation="MTLRSAVVVGLVVWSASAAYADDPAPAPPPGDQPPAEQQPVEQP
                     PVEQPAVEQPPAVKAFDDGRRLLEEGKPGEACARFEESIKLDPDAPGTLLNLGLCNQR
                     LGRTATALGWFRKAQFRAAETQKTDYEDAAKRETATLAAIVPKVHFELANPPPPGARF
                     LLDGNQISENDMGNLEVDPQSHTLEMRVSGKAPLRVEFSAKLDQEQTVLVPVPKTQKK
                     IYVVDHGAPQKALAYLLGGTGVSLYIASGATSLIAKHEFDISEHPEDRQHWKNIARFG
                     GTSLFVVGTAAIVTAAVIYVRAPKAERIERTVLAPTAGDHELGVAVVGSF"
     gene            complement(9216..9590)
                     /locus_tag="VMZ53_33555"
     CDS             complement(9216..9590)
                     /locus_tag="VMZ53_33555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33493.1"
                     /translation="MKWIALLLGVAACQSGNAEQASPSRQAGSSTIISLPKADVTDGY
                     RTDITNLCDAMKQSGAMDHPADERWQVIAMWLGPHITTDAGHEFLVAIQPLNGEPKAL
                     ALESEAKRVGLTNCDLANEWRK"
     gene            9638..10051
                     /locus_tag="VMZ53_33560"
     CDS             9638..10051
                     /locus_tag="VMZ53_33560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3470 domain-containing protein"
                     /protein_id="HUS33494.1"
                     /translation="MAYVVADPCVKCKYTDCVAVCPVDCFYEGKNSIAINPDECIDCG
                     ACEPECPTTAIFEESELPTKWAVYKDINALVSGAKKASEVDTASWPEHLKGAAGSADT
                     WPNITEQKKALPGADDAAKEDNKIVALTLEPGGAE"
     gene            10080..12347
                     /locus_tag="VMZ53_33565"
     CDS             10080..12347
                     /locus_tag="VMZ53_33565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33495.1"
                     /translation="MRLAVYVFLGLALAARSASAIPYESFIDVDDEGDLQDLLASGDI
                     SQDTYDELLDLMSRGVDITTADRNELYTLPNLTYDDVDAIIAYRKLQKGRINDPADLV
                     AAGALSQEKLLAISSFIIIREQADKPFAARGWVRMWTRASPHDFALLDNPATDNGFLV
                     PPFALRARLMAMRNLTAGFAATNTRLDIGEPVYDPNRGALIADARSNQVHVPKAYVKW
                     ESTNLAAIVGSYRVGFGQRLVFDNSSHYSPNGLYLDDQIFYSADLTTACKESAGELLS
                     SPCLGQFGSEYVTPDFAWRDGLFGAAIGAKHLELATGFLQVYGWASLHRRSIYQYELV
                     DHGPNGDKCLDPHDDDNPDCAAPVVYVTPDGDLLTPTTRHSFATLPNVLGEQLFGANV
                     AYFADRRNSVGLTFYTADEKNLINGVDLDLQEWSRIPYGGRYGALGANFSFGSGWLDL
                     FGEAALSFDHIEIQDPSATPARGGGGAAGLVRMTATRKHEELELVGRYYSTDYVNPFA
                     RPISASDEFEGQRARDEAGLRSRYIRTGKDWTLRALVDVWTNPSDYKALKLDTYARAN
                     VRTAEELWLGLWERYQDKNLGEGPPTINGMAPVGPHDQCFEISTDTDENGEPVPCTGR
                     QLTTIARAQYTPDKKMSATVQLEHQLLDDNSKTELKTKFRQDIAAWLIGYYRPTPSDR
                     IRVRVRYLNEAWRDPDYLETSVAANAEYTTKLRDKDTVRIRLDTKFWLDKRMSTADRV
                     PNPEVTLWLFYQASL"
     gene            12359..13300
                     /locus_tag="VMZ53_33570"
     CDS             12359..13300
                     /locus_tag="VMZ53_33570"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUS33496.1"
                     /translation="MPVTPRIKTIVAGVDLSVPSDQALERAAAIAQLNGASLILVHAQ
                     ADDAAIPDADNEVVRQLGEVSAAVRIEEARRLAAKLDDLQARGVNASLVGRAGSPGDV
                     VSEVAKELTADLIVVGTHGHTGIQRFLLGSVATAVLRTSPCDVLVCRGTSTAAAFSRP
                     LVASDFSPAAIRALRNAAALTEPGAPIEVIHAWQLPAGSWGATLLGQARFPWNTVRDA
                     VLSGVKAQAEKLVQTTTDLGHHIHIELVQGPPPSVITHAAERGGHDLIAIGTHGHRGF
                     RRLLLGSVAESVIRHAPCSVLVAHGENAGETSKFVKG"
     gene            complement(13302..13784)
                     /locus_tag="VMZ53_33575"
     CDS             complement(13302..13784)
                     /locus_tag="VMZ53_33575"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HUS33497.1"
                     /translation="MRWALGALVLSSALAACGGDGPCGATEAVVTRVVDGDTIELDDG
                     RKIRLLLVDTPETTGGHDDCYGTNAVTFTSDLVLNKTVQLGFDVECTDRYGRTLAYVT
                     VDGQEVNRLIIQRGFGCVLHISPDGDSRVDEFKGYQVEAKSANRGLWGACMPLPPACT
                     "
     gene            complement(13769..15496)
                     /locus_tag="VMZ53_33580"
     CDS             complement(13769..15496)
                     /locus_tag="VMZ53_33580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33498.1"
                     /translation="MRRLAFLLAAVATGCGPDTTTGACKDNLLPGDLVITEVFADFAA
                     PAGGTGTDDGKEWFEVYNNADRPVSLKGVTVVHSRPDGSKAATHTMTDVTVAPGQFFT
                     LGNSTSDLVPAYVDYGYSADLGDFFNSDGGKLTLKCGDDEIDNAVYEMVKSGHSRQLS
                     NAGPPDYTLNDELVNWCEAKDTEFESGNFGTPGEDNDCAPVIAGACSDGGSMRAVVPP
                     VSGDLVITELMPNPAAVSDTVGEWFEIKAINPIDLNGVKLERLGNTPGSDTLTSSTCL
                     HLAAGSYGVFVKSTDNAMNGMIPAGSIMGVFKFALTASSAPGVIQVSSGGAVLDTVQF
                     TAEPSGKSRQLDPDSTDAIANDAESNFCDGTTPYGAGDLGTPGAANTQCGAVAPPGMC
                     MGTSGLRAIVKPPAGKLVISELMINPMTEVPTGTQEWFEITNTGTTAFDLNGLGIDQA
                     STTRAPDVISSSDCKSVAPNGFALLAHTANTTLPGVDATYGFGLSNSGGELQVLDGTT
                     VLDSVAWTSVTATMYESKSMQLDPDFLDPAMNDLVIGTGSKWCVGATAYDGTNQGTPK
                     AANAQCAGL"
     gene            complement(15511..16602)
                     /locus_tag="VMZ53_33585"
     CDS             complement(15511..16602)
                     /locus_tag="VMZ53_33585"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HUS33499.1"
                     /translation="MSRRPGRLAALVFTSVALVACLHGKSDRYSRKQAQKSLLKLETP
                     GLVVGEFHVTKVSDGDTIRVDGLDSSLRLLGLDTEETFEHYPEQRRQFEADGWQKYTQ
                     EQRAGKAHPVKYATPLGDDAWHWAEKYFNDEKVRIERDHPSEIRDAYNRYLAYVFVKK
                     NGAWVNYNVECVRAGMSPYFPKYGQSRRFHDDFVKAQDEARAAKRGIWAPNVMAYPDY
                     EERLAWWNARGEFMAAFRKEGEGKENYIDISHWDATKKLEDLVGKEVHVVGVVADVRI
                     GEKGPTRVDLARSRFGGFPLVFFDRDVFVQSGIAAWSGEYVVVTGVPTIYENRYNHRK
                     QVQLQIDRAAQITLSPIPGLQIPTSTPSP"
     gene            complement(16608..17180)
                     /locus_tag="VMZ53_33590"
     CDS             complement(16608..17180)
                     /locus_tag="VMZ53_33590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33500.1"
                     /translation="MRLIGISVAAAALAAGCARDPVEHRCPTVGEDDLVVTEFRGQQS
                     PDDGSPTWVELYNASSASVDLEGTKVRFRKKDGSSEVDIIVRRSVIVAAGGYATLGLV
                     PDDAAERPSFIDYGFVDDFHVGFLPAAAVDVEACGTRIDRAIYDVLPKTGSYSLGTNP
                     PNADDNDAPTSWCTNATPAGTPKQANPACP"
     gene            17215..18153
                     /locus_tag="VMZ53_33595"
     CDS             17215..18153
                     /locus_tag="VMZ53_33595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33501.1"
                     /translation="MSVPGMPPPSQPPGYPHQMQPVQPAQPQDPAKTASYMLVGACVL
                     LLIGTFSKSWFGASEGDVSAHFGPMGAEACFHGSCHESGFDGPDVVPLLQIVALLGGV
                     ASAAAAGMFGGMYLANKKDKIPPAVQYRFVPIIFGIAAVGFVGFFLRVVSEGSSKGMG
                     ISWAWVPAFAGVVLAYLGFQRLLPFLPPQGAPRPIGFGQQPNQPGQQPYVQQPQQPYG
                     QQPQQPWGNQSQPMQPQQYGQQPMQQQPYGQQPMQPQQPYGQQPAAQQPVAQQPVAMM
                     AGPGAGAAPNCPRCGAPLQFVAQYQRWFCSRDNQYV"
     gene            18150..18308
                     /locus_tag="VMZ53_33600"
     CDS             18150..18308
                     /locus_tag="VMZ53_33600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33502.1"
                     /translation="MSRGLDRLRTLEDVLAWCRAHGGNIVDVIVQDEFTHDVIVEEPG
                     GFLVFDTT"
     gene            18458..19183
                     /locus_tag="VMZ53_33605"
     CDS             18458..19183
                     /locus_tag="VMZ53_33605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33503.1"
                     /translation="MKIASLFLLVLLLGACGDGAPGKPDARVVPDVAPTPDTPPQPYR
                     HVIMIDGTDDFSPTELFATTSASFTARIAWDDDNLYVAYEGPDLATTTSDADKKWLFV
                     YLDTAAGGETQSEQYNTQRVTFPGTFAADYYARYKVDGTFASLQHGSGGTWTTATPAP
                     TTAQSGMFVELAIPLAAIGAGTSLDLVTYMINEKALAEGTFAGLYAGNFADGYAVNTQ
                     LTTYLHVDFTSQRTPNDAANKRP"
     gene            complement(19180..>19404)
                     /locus_tag="VMZ53_33610"
     CDS             complement(19180..>19404)
                     /locus_tag="VMZ53_33610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33504.1"
                     /translation="LTLYFQSNRTNDQYDIYESTRTTTSSAWSNPTAISDFNTATLNE
                     EDAWLAPSQRTFAYASNAANNQKDVYISTR"
BASE COUNT         3254 a         6378 c         6307 g         3465 t
ORIGIN      
        1 cgctgctctc tgccgttcct cctctgccga tcgcgggcgg cgtcacgctc gatcagctca
       61 ccgcatcact ggtcggtccg atcacggccg tgatcccgtc gggctccgtc gatatccaag
      121 tccacgcgcc gctgagcgac cccaagcctg cacagacgat cgtcgagcac tgcacggaca
      181 tcggcaattt cttcgagctc gcgaaggctc agacgccagg cgcgtgccgc atcgtgctcc
      241 aaggcacgac gcagctcgag ctcgacatct gggtcgacaa caacgagctc cgcctgggcg
      301 cgcacaaggg cgcagcaccg caaggcaagc gcggcggcat gacgtcgacg ggctccgcgc
      361 tcgctgcgtc cgactggacc gctgcgttct ggggccgcgg cacgatgctg aacctgcagg
      421 gaatcgcgcc cgccaccgcg gacgtccccg cagaagtcgc gctcggcatt catgcgatcg
      481 cgctcatcaa cgagctcggt gcagccgcga aagtcgaccc gaccggcgtg acgttccgcg
      541 cgtacctgcg cacggcgtgg acaaacccgc cgggcgtcct cgcgaagatc gccgcaatca
      601 ccggcaacga gatcgtgacg ggcaaggcga ccgagcccgc ggccgcgatc gccacggcca
      661 ctccttcagc gccattcgcg gcagacttcg cagcgggcca aggcggcctg atgatcccag
      721 ctgccgcgat cggcgtggcc gcagcggtcg tcatcccggc catcctgcaa gccgtctcga
      781 atacgcgcgg cgacgcgcaa gcagccaacg cgcctccggt cgacgagcca gcgccgatga
      841 agaacgagga cctcgccgcg ctcctcacgc gcgcctacgt cgaagaagca tggccgcagt
      901 ggaagacgga acacccggac aagccatgtc ccgacaagct cgaagacctc accaagtact
      961 tcggcgagaa ccccggcgtg ccggtgctcg aagacgggtg gggccaccca ctgcgcatga
     1021 cgtgcgaccc gaagttcggc ctcgtcgtga tgtccttcgg gcccgacggc acgttcggca
     1081 cgcccgacga catccacgcg ccctagcgcg cgaccactac ttcgcgggga tggacttcgt
     1141 ccacacctgc atgcggctca tgatctcgct gccggtggag gcgaagttga agcccaagtt
     1201 ctcgaggtgc atcgacgcac gcgtcatgtg ctcgatccac tccgccgtct ccgacagcgc
     1261 gtgcagcttc tggatgtcgc cctgaatcag gaagaagccg ttgaccgtcg agcccggcga
     1321 cggattcgcg aacaccggtt cgaagctgtt gatcgttccg ttgctcttca ggctcgtcag
     1381 atagccgacg aactgcgtga agtgctccgc cgacaacatc tcgcggcccg gaaccgaacg
     1441 cgaccaacta aacagaattg cgtttgccat atcccactcc ttttccatcg cccacgaagg
     1501 gcacgtttgc gggcacggta gtcagcgacg cgtgtcgcct gcaaccggga gtggcagctg
     1561 tccgcgacgc tcacccaaac gggtgcgttt gcgctcacgc tcgtcgaagc agcgcccaaa
     1621 acgcgcgaac cgcgtcgtcc tgatcttcta gctgcgcgta ctccatgaga acggcgacgt
     1681 cgagatcggg aaacaggcgg ctcttcggcc gcacgatgta gcgctcgccc cacagtgcgt
     1741 tgatcgtcaa accgcctttg tcgatgaccc acacctcgcg tacgccgagt cgccggtaga
     1801 tctcgatctt gcgcatcccg tggctatgca cgatctcgat cgcgagatcc ggtgcgcgac
     1861 ctcgctgagc atatcgccga tggcttgcgc cgacgtagta gcactcgtcg ggctcgacgc
     1921 cgaccttctt gcgcttcttg cgcttcgtca tctggccgta gccgtagatt ggaatacccg
     1981 attcgagtgc atacagctcc aagagacgcg cgagcagcgt cttcaggcct ccgtggcgaa
     2041 acccggggct catgatctcg agcgttccgt cgaggtatgc gaaacggacg cccggccggt
     2101 cgacgaacaa gtcattcatc gtgacgtagt cgtcgtatga gattccttcg agaacgatgc
     2161 gctgtccctc taagaagagg tggtcgtcga tatcgtcgat ggcggtcgct gacggcgatc
     2221 ggcgcatgac ggaaagcgta tcactctgaa tctctccggc gggacgtttc aatcgcggcg
     2281 ccttcgcggc tccgcgatct cttcttcgca gtccgcgcac tccatcccga acggctgcag
     2341 ctccgcgtag cggtcgcgag ccgacgcgag ccgcatgaga gcgcgctcga tcatgaggcg
     2401 cgcttcatca cgagcttcgg cctcttcccg cgagccgagc tcgttacctg cgctttgctc
     2461 gcagagcgcg acccacaggt ccagctcgac catcatgagc ttgcccatgt tcacgcactc
     2521 gatcacgcgc gagtacaaga ggtcatcgag cagcttggca gccgctgctt gcgcgatcac
     2581 gacgcacctc ccgcggccgg cggtgtgtgg ccgttcggcg cggcgggcgg ttggccactg
     2641 gcggcacgac gcgcttgcgc gatgagcgcc gcacgcagct gggcttgcat ctgcgggttg
     2701 tcctcgaagt gcgccatcgc cttctccttg ccttggccga tcttggcgcc gccgtacgag
     2761 taccaggtac cggacttctc gacgaggccg tgcttctcgg cggtgtcgac gagctcgccg
     2821 agcttgttga cgcccgtgcc gtagaggatc tcgaactcgg cttcgcggaa cggcggcgcg
     2881 agcttgttct tgacgacttt gactttgacc tcgctgccga tcgtgtcgtc gccgcgcttg
     2941 atcgctttct tgcgtcggat gtcgaggcgg accgagcagt agaacttgag cgcgttgcca
     3001 ccggtcgtcg tctcggggtt gccgtagacc acgccgatct tttggcgcag ctggttgatg
     3061 aagatcacgc aggtgcgcgt gcgcgagatg accgccgtga gcttgcgaag cgcttgcgac
     3121 atcaagcggg cttgcaggcc catgtgcgag tcgcccatgt cgccctcgat ctcggcgcgt
     3181 ggcgttagtg ctgcgacgga gtcgacgacg atgagatcga tcgcgccggt gcggacgagc
     3241 gtgtcgcaga tttcgagcgc ctgttcgccg cagtcgggct gcgatacgag caggtcttcg
     3301 agatcgacgc cgaggcggcg ggcgtagtcg gtatcgagcg catgctcggc atcgatgaac
     3361 gcgcacacgc cgccggcgcg ctgagcttcg gcgatcgcgt gcagcgtgag cgttgttttg
     3421 ccggacgact ccgggccgta gatctcgacg atgcggccgc gtggtaggcc accgcagccg
     3481 agtgcgacgt cgagcgcgac cgagcccgtc gggatgacct cgacagcggc gacttcattg
     3541 ccaccgtcga ggcgcatgat cgagcctgag ccgaactgct tgtggatcgc gcgaatcgct
     3601 tcgctgatcg atttgttctt cttttcttga gcggacatgt tcgaacctcc ggcggcgcgc
     3661 atctacatga cgcgtgccat ccgcaagtcc gcgggaatgc gtgagcgcgt gtccggagtg
     3721 tccggaaccc ggacgacggt gtccgggtgg cggcggacag cttaaggctt gacgttgaag
     3781 gcggcggtga acgtcgtcgc ctgcgtgaac gggaacgtga cagtgtccgc gccgtagttg
     3841 ccctgcttcg ccttcgagag gccgctacgc gcttggatcg agaacacgta ctggtgactc
     3901 ggcgagagca ggcttccgtc gacggtcacc gagggcgtca gcacgtgata gatgcgaatc
     3961 ggcgcgaggc ggttggcggt gatctcgtac agcgtgacga tgtaatcgtc gccgttaccg
     4021 accgagggtt cgtctgccca tgtcagcgcg gtcgcctgcg agctcgggtt gatcatgagg
     4081 ccgtcagcgg tcgagaggtc gttcgtgccg agcttgatct ggcgggcgag cggcgctttg
     4141 acctcgactt ggccggtgaa cacgttcgtc atcacttcca tcgccgtata gaggtcagcg
     4201 cccgacaccg tgcgcttcgt cgcgaactga accgtgacca cgcgatcgaa cggaagcatc
     4261 gacgtcggat cggcgagcgt catcgacgtg acgatcgacg gcgcggtgag gaacgggatg
     4321 atgagcggct gcgcgagaaa gttttgtagc gacgttcccg gaacgaacgc ggggagcttc
     4381 gtcgtcggcg agatgccgta cgacattgtc tgcgtgcgcg tgccggcgcc gatcgagccg
     4441 agggcgtcga accggttgtt cgcggtcgtg acatcgatgt tcgggaagca gtccggacac
     4501 gcgagcgctg ttccgaggtt gcgattgacc gccgagccag acatccacgc cggctgcgtt
     4561 tgcggcggcg actgcgtgcc cgcagcgagg tcgatatgcg tcagtgcgaa gccgccgagg
     4621 gattcgtagc cggtgtgcgc gacccattca ccgaggatga cgtagtcacc gagcgagccc
     4681 tgcggtgcgc cacgcggcgt cgtcatcatg tccgcgttcg cagcgaagtc atacgtcgtc
     4741 gtcgcgccgg agcgcgggaa cgggccggtg cgatcctgct gcgtgaacac gccggtcgtc
     4801 gcgatcgatg gcgacgtcag cgagcccgag aggccggtcg gcgtgatcgt gtagccggcg
     4861 ccggcaggaa tgttcggcgc gtcgaagcgt gtcgtgcgcg ggatgacgat gaccgcgccc
     4921 gcgatcgacc actggatctc gtgcggcacc gtctcgttcg gcagcgtgta gacgatgcgg
     4981 tgcggcgcct cgcgcaagtt gtatggaatc tggaacgagc catcggtgat gtcgtagttg
     5041 gcggccatca gcggcccgtc ggcgagcggc ggccccacga agatcgtcgg caccatgtcg
     5101 cgcgccgatt cagagccgat cgctttatag atgggagcga tgggcgcgcc cgtggtgttc
     5161 gtcgccgcga tgccccacag cagccgatct tcgacttcga acggcgcgtc cggcgagtca
     5221 ggagccgcgt cgtacggaat cgtcggcttc aggtcgaaga cctgattaca gccggaggct
     5281 gcaacgagca gcgcgagggc aagctgcctc atcgccgcga atcgtggcac gggccgcgcg
     5341 gaggacacaa catctgtgct tcagtccaca cgtgaagctg gacgcgcgcc tgaacctctt
     5401 catcacgctc gttgccgtgt tcatggtctg cctcgtcgtc ggcgacatca tcggcggcaa
     5461 gctgacgtcg tttcacctgt tcggacgcga gtggatcttc tcggtcggcc agatcgcgtt
     5521 tcctgtcacg ttcatcctga cggacatcct gaacgagttc tacggccgga aagtcgttcg
     5581 ccggatcacg ttcctcgcgt tcttcatggt cgggctcacg ttctgcatca tctttattgc
     5641 gggcaagctg ccgtgggcgc cgtttacgcg cgacccgagc tggaagggcg tcaacccgag
     5701 ccaattcgag gtcgtgttca cgcaggcgag cgacatccag atcgcttcga tgttcgcgtt
     5761 cctgaccggc aacatggtcg acatcagcgt cttcttcctg ctgaagcgcg caaccgggag
     5821 ccgcttcctg tggctccgcg cgaccggctc gaccgcggtc tcgcagctca tcgacacggt
     5881 gatcatctcc ggcatcatct ggtacggcgt ccacttccag ctcggctggc cgccggtgtt
     5941 catcgatgcg ccgaaggtga cgtttgcgca gttcgtgacg atcgtgatga cgtcgtactt
     6001 gatcaagctc gtcgccgcga tcggtgtgac gccgatcatc tatggcctgc acgagctgat
     6061 cgaaaagaag ttcggaatcc atccggcgcc cgccgaggtc gagagcgccg atctcgaagg
     6121 ccgcgacggc gttcagagcg attcgaagta gagatcctgc tgcacgatgc ggccgagcgt
     6181 tcccgcgata gcgtcgcgcg cctcggcgaa gcctctcgtc cggaatccgg cgaagccgcc
     6241 cggcagcgag atcgtctgca gctcctcgac ggcgaccgtg tcaccggcgg ccggcgtgaa
     6301 atcgaagttg tcgacgatcg gcagctgcgg atacgtgaca gacgtaccgc tgcgcgccgc
     6361 aacgatgcgc caggtccagg cagtgcccgt cggaatcgca tcgcgataga cgtggatgcg
     6421 cgcgcggacg acatcgggct ggatatcgcc cgggcgctcc gtccacgaca gcgtgtgcgt
     6481 cggttgcgcg aagaccggcg cggtcgcgaa tggcggcagc agcacggtgt cgagatcgag
     6541 gtcgtacgtc gtcgtcgccg cgtgccagtt gtagatcgtc tggattccga tctcgccgct
     6601 cgtcgggtac aagttcgtcg ccgtcagctc cgtcgcggac gtcgttggca tcgagacggt
     6661 cgccgtcgcc gcatgcgcta cgggagcctc gcctgccgtg accgcgaacg cgcgccgcgt
     6721 tgccgacagc gattcgtaca tgccgatgaa cgacacgaaa tcgggcacgt tcgtgtagcg
     6781 cagctggacg gatcgcaacg gctgatacgg actgctcgtc agatctaccg gaccgtcgac
     6841 gctgacgttt ggcgcgaaca gactgccgag cggattgccg tcgacatccg ccggaacgat
     6901 cacgagatca gtgaccgcac cgcacccgac gagacgaata tcgtcggtcg ccatcgccat
     6961 cggcgcttca ccacatggcg agtacacgtt gtacgcgacc gcgttcgaga gcgccgggac
     7021 ctggacgtgc accatcgctt gcgtggtgtc gcccgtcggc gagaacgtga gctcgaggga
     7081 gtcggccggc tgcacgcccg cgaacgtgct gagctcctcg acctgctggc ctttgtgctc
     7141 gcgcgcggtg acgaagccac cgttcggcat gatcgcccac gcgacgccgc tcgcgttcgt
     7201 cagcttctgt gcgatcagcg acgagtccgc ggcctggaag aacacggcga catcggcaac
     7261 cggcgcaccg acgcgtgtca ccgtcagctt cacggcgttc ggtggtgcct cgccggtctc
     7321 cgacgacagc gaatcgatcg cggtgtcgag cggctgcgcg tcgacggtct gatgcaggtc
     7381 cgccttgcac gcgacgagcg acaacgcgag gacagcgcgc ttcatcggtc gtatctccgc
     7441 atgaggccgt cgtggatcgt gtagagccgc tcgcaatgac cgagcagctg cggcgcttca
     7501 tcgttgccgt tggcgaggat cttcaccggc gtgccgaacc actcgtccat cgacggacgc
     7561 gtcatgccgt agacctccag cgcatcgccc gactcggtga gcgtgcgacg tgtgaacacg
     7621 gcagtgaggc cgttcggcgt taggttcggt ggcatctcgc tgtcgaagtc gtgcgcgcca
     7681 ccgacgggaa cccacgtgcc gttgtcgtct tcgagctcct gcaccgttgc atcgttcatg
     7741 tgcatgcgcg cgagcacgcg ccgcgggccg aacacatcgg cggtcggcgt tccgacgaac
     7801 gtcgcgatcg gcgtgcgcgc ctcgagcgtc cacgatccgg gcgctccgcg cagcgcgcct
     7861 tcgagcaccg gcggttccgt cgacgacgtg tagagcagcg catcgccctc gggtgacagc
     7921 gcggcgttga gggtgatgta cggtccgagg tcgaccttgc ggggtgtccc gctcggcagc
     7981 tcgagctcgg catgcgagcc atcgtcgagg acggcgaccg cgaagcgctg atcccaatcg
     8041 acggagaagc gaaacgccgg aatgatgtcg gtcgcgcgcg cgctgtcgaa cgcttggagc
     8101 tcgcaaggct cgacctcacc gccgacgcca aacgcgaggt cgcagccgcc gagcagcagc
     8161 aagaggacgc tagaacgagc cgacaacggc cacccccagc tcgtgatctc cggcggtcgg
     8221 cgcgagcacg gtgcgctcga ttcgctcggc ctttggcgcg cgcacgtaga tgaccgcggc
     8281 cgtcacgatc gcggccgttc cgacgacgaa cagcgacgtg ccgccgaacc gcgcgatgtt
     8341 tttccaatgc tggcgatctt cgggatgctc gctgatatcg aactcgtgct tcgcgatgag
     8401 cgacgtcgcg ccgctcgcaa tgtagagact cacgcccgta ccaccgagca aatacgcgag
     8461 cgccttttgc ggcgcgccgt gatcgacgac gtagatcttc ttctgcgtct tcggcacggg
     8521 cacgagaacg gtctgctctt ggtcgagctt cgccgagaat tcgacgcgga gcggcgcctt
     8581 gcccgacacg cgcatctcga gcgtgtggct ctgcggatcg acctcgagat ttcccatgtc
     8641 gttctcggaa atctggtttc cgtcgagcaa gaacctcgcg ccgggcggcg gtgggttcgc
     8701 gagctcgaag tggaccttcg gaacgatcgc agcgagcgtc gcggtctccc gcttcgccgc
     8761 gtcttcgtag tccgtcttct gcgtttccgc cgcgcgaaac tgcgccttgc gaaaccagcc
     8821 gagcgccgtc gcggtgcgtc cgagccgttg gttgcagagg ccgaggttca gcagcgttcc
     8881 gggcgcatcg ggatcgagct tgatcgattc ttcgaagcgc gcgcacgcct cgccgggctt
     8941 gccttcctcg aggagccggc ggccgtcatc gaaggccttc accgcaggcg gctgctcgac
     9001 cgctggctgc tcgaccggcg gttgctcgac gggctgctgc tcggcgggcg gttgatcacc
     9061 cggcggcggc gctggagctg gatcgtcggc gtatgcggcg ctcgcgctcc acaccaccaa
     9121 ccccacgacg accgcgctgc gcaatgtcac caaggctcct catcgtaaca gcctcggcgc
     9181 gaatcccgac ggagccctac ctcgatgcta accgactact tgcgccattc gttggccaga
     9241 tcgcaattgg tgagcccaac gcgctttgcc tcactctcga gcgcgagcgc tttgggctct
     9301 ccattgagcg gctggatggc cacgagaaat tcgtgtcccg catcagtggt gatgtggggg
     9361 ccaagccaca tcgcgatcac ctgccagcgt tcatctgctg gatggtccat ggctccggac
     9421 tgcttcatgg cgtcgcagag gttcgtgatg tctgttcggt agccgtccgt cacgtcggct
     9481 ttgggcaggc tgatgatggt cgacgagccc gcttgccgag acggcgacgc ctgttcggca
     9541 ttccccgact ggcaggccgc cacgcccagt agaagcgcga tccacttcat gcggcgacac
     9601 tctgcacttt cggacgaagt ttggctagat tgcgcccatg gcatacgtcg tcgctgaccc
     9661 gtgcgtgaag tgcaagtaca ccgactgcgt tgcggtgtgt ccggtcgact gcttctacga
     9721 gggtaagaac tcgatcgcga tcaaccccga cgagtgcatc gattgcggcg cgtgtgagcc
     9781 cgagtgcccg acgaccgcga tcttcgagga gagcgagctc ccgacgaagt gggcggtcta
     9841 caaggacatc aacgcactcg tcagcggcgc gaagaaggcg tcggaagtcg ataccgcatc
     9901 gtggcccgag cacctcaagg gtgctgctgg ctccgccgac acgtggccga acatcacgga
     9961 gcagaagaag gctcttccgg gcgccgacga cgccgcgaag gaagacaaca agatcgtcgc
    10021 gctgacgctc gagccgggcg gcgcagagta atcagagtcc gtgatagcgt cggggtcggg
    10081 tgcgtctcgc ggtctacgtc ttcctcggcc tggcgctcgc tgcgcggtcc gcatcggcca
    10141 ttccgtacga gtcgttcatc gacgtcgacg acgaaggcga cttgcaggac ctgctcgcct
    10201 ccggcgacat ctcgcaggac acctacgacg agctcctcga cctcatgtcg cgcggcgtcg
    10261 acatcacgac cgccgaccgc aacgagctct acacgctgcc aaacctgacg tacgacgacg
    10321 tcgacgcgat catcgcgtat cgcaagctcc agaaggggcg catcaatgat ccggcggacc
    10381 tcgtcgctgc gggtgcgctg tcgcaggaaa agctcctcgc gatctcgtcg ttcatcatca
    10441 tccgcgagca ggctgacaag ccgttcgcgg cgcgcggctg ggtgcggatg tggacgcgcg
    10501 catcgccgca tgacttcgcg ctgctcgaca atccggcgac ggacaatggc ttcctcgtgc
    10561 cgccgttcgc gctgcgcgct cggctcatgg cgatgcgcaa tctcaccgct ggcttcgcgg
    10621 caacgaacac gcgcctcgac atcggcgagc ctgtgtacga tccgaatcgc ggcgcactga
    10681 tcgccgacgc gcgctcgaac caggtgcacg tgcccaaggc gtacgtgaag tgggagagca
    10741 cgaatctcgc ggcgattgtc ggctcgtatc gcgtcggctt cggccagcgg ctcgtattcg
    10801 acaactcgtc acactactcg ccgaacggcc tgtacctcga cgatcagatc ttctactcgg
    10861 cggatctcac gacggcctgc aaggagtctg caggcgagct gctgtcgtcg ccgtgcctcg
    10921 gccagttcgg ctcggagtac gtgacgccgg acttcgcgtg gcgcgatggc ctgttcggcg
    10981 ctgcgatcgg cgcgaagcac ctcgagctcg cgaccggatt tctccaagtc tacggctggg
    11041 cgtcgctgca tcgccgcagc atctatcagt acgagctcgt cgatcacggc ccgaacgggg
    11101 acaagtgcct cgacccgcac gacgacgaca atcccgattg cgccgcgccc gtcgtgtacg
    11161 tcacgcccga tggcgatctc ctgacgccga cgacgcgcca ctcattcgcg acgctgccga
    11221 acgtcctcgg cgagcaactg ttcggcgcga acgtcgcgta cttcgcggat cgccgtaact
    11281 cggtcggcct cacgttctac accgccgacg aaaagaacct gatcaacggc gtcgatctcg
    11341 atctccaaga gtggtcgcgc attccatacg gcggccgcta cggcgcactc ggcgcgaact
    11401 tctcgttcgg ctcgggttgg ctcgatctgt tcggcgaggc cgcgctcagc ttcgatcaca
    11461 tcgagatcca agacccgtcg gcgacgccgg cgcgcggcgg tggtggcgca gcaggcctcg
    11521 tccgcatgac cgcgacgcgc aagcacgaag agctcgagct cgttggccgc tactactcga
    11581 cggactacgt gaatccattc gcgcggccga tctctgcatc cgacgagttc gaaggccagc
    11641 gcgctcgcga cgaagcgggc ctgcgctcgc gctatatccg cacgggtaaa gactggacgc
    11701 tgcgcgcgct tgtcgacgtg tggacgaatc cttccgacta caaggcgcta aagctcgaca
    11761 cgtacgcacg cgcgaacgtc cgcaccgcag aggagctgtg gctcggcctg tgggaacgct
    11821 accaagacaa aaatctcggc gaagggcctc ccacgatcaa cgggatggcg cctgtcggcc
    11881 cgcacgatca gtgcttcgag atctcgaccg acaccgacga gaacggcgaa ccagtcccgt
    11941 gcacgggccg ccagctcacg acgatcgcgc gcgcgcagta cacgcccgac aagaagatgt
    12001 cggcgaccgt gcagctcgag caccaactgc tcgacgacaa ctcgaagacg gagctcaaga
    12061 cgaagttccg ccaagacatc gctgcatggc tgatcggcta ctaccgtccg acgcccagcg
    12121 atcgcatccg cgtccgcgtg cgctacctca acgaggcgtg gcgggatccg gactatctcg
    12181 agacgtctgt cgcggcgaat gccgaataca cgacgaagct tcgcgacaaa gacaccgtgc
    12241 gcatccgcct cgatacgaag ttctggctcg acaagcgcat gagcaccgcc gaccgcgtgc
    12301 cgaatccaga ggtcacgctt tggctgttct atcaggcgtc gctgtaggct agaccaccgt
    12361 gccagtgaca cctcgcatca aaacgatcgt cgccggcgtc gacctctccg tgccgagtga
    12421 tcaagcgctc gagcgcgctg ccgcgatcgc gcagctcaac ggcgcctcgc tgatcctcgt
    12481 ccacgcgcaa gccgacgacg cggcgattcc cgacgccgac aacgaggtcg tccgccagct
    12541 tggcgaggtg tccgccgcgg tgcgcatcga ggaagcacgt cgcctcgccg cgaagctcga
    12601 cgatctgcaa gcgcgcggcg tcaacgcgag cctcgtcggc cgtgcgggat cgccgggcga
    12661 tgtcgtgagc gaagtcgcca aagagctcac cgctgacctg atcgtcgtcg gtacgcacgg
    12721 gcataccggc atccagcggt tcttgctcgg cagcgtcgcg acggcggtct tgcgcacgtc
    12781 gccgtgcgat gtcctcgtgt gtcgcggcac gagcaccgct gctgcgttct cgcgtccact
    12841 cgtcgcgagc gacttctcgc ccgccgcgat ccgcgcgctc cgcaacgccg ctgcgctcac
    12901 cgaaccgggc gcgccgatcg aagtcattca tgcatggcag cttcccgcgg gctcgtgggg
    12961 cgcgacgctc ctcggtcagg cgcgctttcc gtggaacacc gtgcgcgatg cagtgctctc
    13021 cggcgtgaag gcgcaagcgg agaagctcgt gcagacgacg acggacctcg gccatcacat
    13081 tcacatcgag ctcgtgcagg ggccgccacc gtcggtgatc acgcacgcag cggaacgtgg
    13141 tggtcacgat ctgatcgcga tcggcacgca cggtcatcgc ggctttcgcc ggctcctgct
    13201 cggcagcgtc gccgagagcg tgatccgtca cgcgccgtgc tcggtgctcg tcgcgcatgg
    13261 cgagaatgcc ggcgagacgt cgaagttcgt gaagggttag ctcacgtgca cgcgggaggc
    13321 agcggcatgc acgcgcccca caggccgcga ttcgccgact tcgcctcgac ctgatagccc
    13381 ttgaactcgt cgacgcgcga gtcgccgtcg ggcgagatgt gcagcacgca gccgaagccg
    13441 cgctggatga tgaggcggtt gacctcttgg ccatcgacgg tgacgtacgc gagcgtgcgg
    13501 ccgtagcggt cggtgcactc gacgtcgaag ccgagctgca cggtcttgtt cagcacgaga
    13561 tcgctcgtga acgtgacggc gttcgtgccg tagcaatcgt cgtggcctcc cgtcgtttcg
    13621 ggcgtgtcga cgaggaggag gcggatcttg cggccgtcat cgagctcgat cgtatcgcca
    13681 tcgacgacgc gcgtgacgac cgcttcggtc gcgccgcacg gcccatcgcc accgcatgcg
    13741 gcgagcgccg acgagaggac gagcgcgcct agagcccagc gcattgcgcg ttggccgctt
    13801 tgggagtgcc ttgattcgtg ccgtcgtatg cggtcgcgcc cacgcaccac ttcgaccccg
    13861 ttccgatcac gaggtcgttc atcgctggat cgaggaagtc gggatcgagc tgcatcgact
    13921 tgctctcgta catcgtcgcg gtgacgctgg tccacgccac gctgtcgagc acggtcgttc
    13981 cgtcgagaac ctgcagctca ccgccggagt tcgacagacc aaagccgtac gtcgcatcga
    14041 caccgggcag cgtcgtgttc gcggtgtgag cgagcagtgc gaagccgttg ggcgcgacag
    14101 acttgcagtc gctgctgctg atgacgtcag gtgcgcgcgt cgtcgacgcc tggtcgatgc
    14161 cgaggccgtt caagtcgaat gccgtcgttc ccgtgttcgt gatctcgaac cactcttgcg
    14221 tgcccgtcgg cacttcggtc atcggattga tcatcagctc gctgatgacg agctttcctg
    14281 ccggcggctt gacgatcgcg cgcaggcccg aggtgcccat gcacatgccc ggcggtgcaa
    14341 cggctccgca ctgcgtgttc gcggcgcccg gcgtaccgag gtcacccgcg ccatatggcg
    14401 tcgtgccgtc gcagaagttg gattccgcat cgttcgcgat cgcgtccgtc gaatcgggat
    14461 cgagctggcg cgacttgccg ctcggctccg cggtgaactg aacggtgtcg agcacggcgc
    14521 caccggagct gacctggatg acgccgggcg ccgacgaagc ggtgagcgcg aacttgaaca
    14581 cgcccatgat cgatcccgca gggatcatgc cgttcatggc gttgtccgtc gatttgacga
    14641 acacgccgta gctgcctgct gccaggtgca agcacgtcga cgacgtgagc gtgtcacttc
    14701 cgggtgtgtt gccgaggcgc tcgagcttca cgccgttgag gtcgatcggg ttgatcgctt
    14761 tgatctcgaa ccactcgccg accgtgtcgc tgaccgcggc agggttcggc atgagctcgg
    14821 tgatcacgag atcaccggag accggcggga ccacggcgcg catcgagcca ccgtcgctgc
    14881 acgcaccggc gatcacgggc gcgcagtcgt tgtcctcacc gggcgtaccg aagttgcccg
    14941 actcgaactc cgtgtccttg gcttcgcacc agttcacgag ctcgtcgttg agcgtgtagt
    15001 ccggcgggcc agcgttcgag agctggcgcg agtggccgct cttgaccatc tcgtagacgg
    15061 cgttgtcgat ctcgtcgtca ccgcacttga gcgtgagctt gccgccgtcg ctgttgaaga
    15121 aatcgccgag gtcggcggag tagccgtagt cgacgtacgc gggaacgagg tcactcgtcg
    15181 agttgccgag cgtgaagaac tggcccggcg cgacggtgac gtcggtcatc gtgtgcgtcg
    15241 ccgccttcga gccgtcgggg cgcgagtgca cgaccgtgac gcccttgagc gacaccgggc
    15301 gatccgcgtt gttgtagacc tcgaaccatt ccttgccgtc atcggtgccg gtgccgcccg
    15361 caggcgcggc gaagtcggcg aacacttcgg tgatgacgag gtcgcccggc aggaggttgt
    15421 ccttgcacgc accggtcgtc gtgtcgggcc cgcagccagt tgcaacggcg gcgagcaaga
    15481 aggcgagacg gcgcatcccc ttacgataga tcatggcgac ggtgtgctgg tgggaatctg
    15541 caggccggga atgggagaaa gcgtgatctg cgccgcgcgg tcgatctgga gctgcacttg
    15601 cttccggtgg ttgtaccggt tctcgtaaat cgtaggcacg ccggtcacga cgacgtactc
    15661 gccgctccat gccgcgatgc cgctttgtac gaaaacatcg cgatcgaaga acacgagcgg
    15721 gaagccgccg aaccgcgagc gagccaagtc gacgcgcgtc gggcctttct cgccgatccg
    15781 cacgtcggcg acgacaccga ccacgtgcac ctctttgccg acgagatcct cgagcttctt
    15841 cgtcgcgtcc cagtgcgaga tgtcgatgta gttctctttg ccttcgcctt ccttgcgaaa
    15901 tgccgccatg aactcgccgc gcgcgttcca ccatgcgagg cgctcctcgt aatcgggata
    15961 cgccatcacg ttcggcgccc agatgccgcg cttggccgcg cgcgcttcgt cctgcgcctt
    16021 gacgaaatcg tcgtggaagc gacgcgactg cccgtacttc gggaagtatg gcgacatgcc
    16081 ggcgcgcacg cactcgacgt tgtagttcac ccacgcgccg ttcttcttga cgaacacgta
    16141 cgcgaggtag cggttgtacg cgtcgcgaat ctccgacgga tgatcgcgct cgatccgcac
    16201 cttctcgtcg ttgaagtatt tctccgccca gtgccacgca tcgtcgccga gcggcgtcgc
    16261 gtacttcacg ggatgggctt tgcccgcgcg ctgctcttgc gtgtacttct gccagccatc
    16321 cgcctcgaat tgacgccgct gctccgggta gtgctcgaac gtctcctcgg tatcgaggcc
    16381 gagcagacgc agcgagctgt cgagcccgtc gacgcggatc gtgtcgccgt cggagacctt
    16441 cgtgacgtgg aactcgccga cgacgaggcc cggtgtctcg agcttgagga gcgacttctg
    16501 cgcttgcttg cgcgagtagc gatcggactt gccgtggagg catgcgacga gcgcgacgct
    16561 cgtgaagacg agtgcggcga ggcgaccggg gcggcgagac atagacgtca aggacatgcg
    16621 ggatttgcct gcttgggagt accggccggg gtggcgttcg tgcaccagct cgtcggagcg
    16681 tcgttgtcat cggcgttcgg cgggttcgtg ccgagcgagt agctgccggt cttcggcaag
    16741 acgtcgtaga tcgcgcgatc gatgcgcgta ccgcacgctt cgacgtcgac ggcggctgcg
    16801 ggcaagaagc cgacgtgaaa atcgtcgacg aatccgtagt cgataaagct cggccgctct
    16861 gctgcgtcgt cgggaacgag cccgagcgtt gcgtagccgc cggctgccac gatcacgctg
    16921 cgccggacga tgatgtcgac ctcggagctg ccgtctttct tgcgaaagcg gaccttcgtg
    16981 ccctcgagat cgacgctggc cgacgacgcg ttgtagagct ccacccacgt cggcgagccg
    17041 tcgtcggggc tctgctggcc gcggaattcg gtgacgacca ggtcgtcctc accgacggtg
    17101 gggcagcgat gctcgacggg atcacgggcg caccctgccg caagcgccgc tgcggcgacg
    17161 gaaatcccga tcaagcgcac ggccatactg taacgcaggt atgctcgccg ggtcatgagc
    17221 gtccccggca tgccacctcc gtcacaacca cccggctacc cacaccagat gcaacccgtg
    17281 cagccggcgc agccgcagga tccggcaaag accgcctcgt acatgctcgt cggtgcgtgc
    17341 gtgctgctcc tgatcggcac gttctcgaag agctggtttg gcgccagtga aggcgatgtg
    17401 agcgctcact tcgggccgat gggcgccgag gcgtgctttc acgggagctg tcacgaatcc
    17461 ggcttcgacg gtccggacgt cgttccactc cttcagatcg tcgcgctcct cggtggcgtc
    17521 gcgtccgcgg ccgcagccgg catgttcggc ggcatgtacc tcgcgaacaa gaaggacaag
    17581 atcccgccgg cagtccagta ccgcttcgtc ccgatcatct tcggcattgc cgcggtcggc
    17641 ttcgtcggct tcttcctgcg cgtcgtctcc gaaggcagca gcaagggcat gggcatcagc
    17701 tgggcatggg tgcccgcgtt cgccggtgtt gtcctcgcgt acctcggctt ccaacgcttg
    17761 ctgccgttct tgccgccgca aggcgcaccg cggccgatcg gcttcggcca gcagccgaat
    17821 caacctggcc aacagccata tgtccaacag ccgcagcagc cgtacggcca gcaaccgcag
    17881 cagccgtggg gcaatcaatc gcagccgatg cagccgcagc aatacggcca gcagccgatg
    17941 cagcagcagc cgtatggcca acagccgatg cagccgcagc agccgtatgg ccagcagccc
    18001 gcggcccagc aacccgtcgc gcagcaaccg gtcgcgatga tggcaggccc cggcgccggc
    18061 gccgctccga attgcccgcg ctgcggagcg ccgctgcagt tcgtcgcgca gtaccagcgc
    18121 tggttctgct cgcgcgacaa tcagtacgta tgagccgcgg cctcgatcgt ctccggacgc
    18181 tcgaggatgt gctcgcgtgg tgccgcgccc atggcggcaa catcgtcgac gtgatcgtcc
    18241 aagacgaatt cacgcacgac gtgatcgtcg aggagccggg tggctttctc gtcttcgaca
    18301 cgacctgact cggcggcgtg aacgcggtct ccgtgtggga tcaccgaccg accgccgacg
    18361 aagcgctcga cgcgcggatc gccgggggtt ggaaggcgac gcccaccggc accgtcgacg
    18421 gaccggtcgt gctcgggcat gctgcatgtc gattccgatg aagatcgcat cgctgttcct
    18481 gctggtcttg cttctcggtg cgtgcggcga tggtgcgccc ggcaagcccg acgcgcgtgt
    18541 cgtccccgac gtcgctccga cgcctgacac gccgccacag ccgtaccgcc acgtgatcat
    18601 gatcgacggc accgacgatt tctcgccgac cgagctgttc gcgaccacgt ccgcatcgtt
    18661 caccgcgcgc atcgcgtggg acgacgacaa cctctacgtt gcctacgaag ggccggacct
    18721 cgcgacgacg acgagcgacg ccgacaagaa gtggctgttc gtctatctcg atacagcggc
    18781 aggcggcgag acgcagagcg agcagtacaa cacgcagcgc gtgacgttcc cgggcacctt
    18841 cgcggccgac tattacgctc gctacaaagt ggacggcacg ttcgcatcgc tccagcatgg
    18901 aagcggcggt acgtggacca ccgcaacacc cgcaccgacg accgcgcagt ccggcatgtt
    18961 cgtcgagctc gcgattccac tcgcggcgat cggcgccggg acgtcgctcg atctcgtcac
    19021 gtacatgatc aacgagaagg cgctcgctga aggcacgttc gccggtctgt acgccggcaa
    19081 cttcgccgat ggctacgcgg tcaatacgca gctaacgaca tatctgcatg tggacttcac
    19141 gtcgcagcgc acgccgaatg acgccgcgaa caagcggccc tagcgcgtcg agatgtagac
    19201 gtctttttgg ttgttcgccg cgttgctcgc gtatgcgaag gtgcgctggc tcggcgcgag
    19261 ccaggcatct tcttcgttga gcgtcgccgt attgaaatcg ctgatcgccg tcggattgct
    19321 ccacgccgac gacgtggtcg tgcgcgtcga ctcgtagatg tcgtactggt cgttcgtgcg
    19381 attcgactgg aagtagagcg tgag
//
LOCUS       DATLBZ010000685         5160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10244220, whole genome shotgun sequence.
ACCESSION   DATLBZ010000685 DATLBZ010000000
VERSION     DATLBZ010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10244220"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VMZ53_33615"
     CDS             <1..604
                     /locus_tag="VMZ53_33615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33505.1"
                     /translation="DARRAVVYFESVNTSGCHQFDSNYGNNYPFDCATPPQWVGNAST
                     LTTRDTSGDICGGAPANQSFGFDTWVRQRAAITNLCFEVYQPGMTDRDDPDIWQKLDV
                     AIHWREASSLTWQSQPVNFDRRVGNNARFAFNWRQVDPFRFAHCPTGTVTTTPDGYVQ
                     ETVEYYVTVNGYQLRPVPGGAYAGIFTDYNNDAWRAANCH"
     gene            646..2091
                     /locus_tag="VMZ53_33620"
     CDS             646..2091
                     /locus_tag="VMZ53_33620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUS33506.1"
                     /translation="MPERLLKTTTSLCRACKRAAPAEIWRIGTTVVMRKRCEVHGASE
                     VEVASNADWYERTVGYAPVLAKPEAKKAVSQGCPFDCGPCTSHEQQSQLPIVPITSAC
                     NLDCPICYTHNKNGEGAFHMTETQLAAILEHLRAADPERRIINLTGGEPTQHPQFERI
                     VERCVEEGIHRITVSTHGLRFLKDERLLERLAKLGARIVLSFDSFKPETNQHMLGGNF
                     LDGKLRVLDLLEKYQVETTLLPVLARGVNDDEVGAFVRLALEKPFIRSLELHTMTFTG
                     ANGQTFDRGGRYSTFDVLTDIEAQTGGMLRVSDFVPSPAAHPLCYLVTYVLKLDEGRW
                     LPFPRFMSGEDLRELLAGMLYLEPTRATEEKLGDVIERLWAGDIDCDDSELVLSRLRA
                     LTESVFARGVEPAERLRRAEMSSKAIYIHAHMDEDTFDTDRIRQCCVGIREPDGTNIP
                     SCAYNTLYRDRDARFAEKPASPLVTLGLGRR"
     gene            2095..3183
                     /locus_tag="VMZ53_33625"
     CDS             2095..3183
                     /locus_tag="VMZ53_33625"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HUS33507.1"
                     /translation="MSSSRDDVAAPPDAAAAPPSTAAAAAPKHRLWWILLLVVVGGGG
                     IYLGAPNSDVLRVAWARLSSVRGAAIAIVVGGALGLVATEALRIAVIGRLVGVRVSAR
                     DAWDAAVANHVMTAVTPQVGLGEPTVAYTLGRRGTPWDAAVAIPFIKFTSSLVLVFVL
                     GAALVGAGFGPVVDGRIGALGIVWFLAIAAITSLVIAICSSPSTARRWIGRIAGWLSR
                     RRWFASPAWQARIENSAQVTIRTADRLAGVRRASWRSAIALVAVHLIYYASYVAPLVC
                     LACVLGDPPVITLALRSLVYLCFLFAMPTPGGTGPGEAAAAVFFGDLVPPADAIVIVA
                     VFRAATFYLQLAIGALYLPIRSLALSRR"
     gene            3180..3971
                     /locus_tag="VMZ53_33630"
     CDS             3180..3971
                     /locus_tag="VMZ53_33630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HUS33508.1"
                     /translation="MSGLDWVAYIAMGVMFVAFTGEVITSYFIRKDGYYEKNDTLVNL
                     AVALGYVVASMSYGVLIVLILVPVWKLTPFRWSMDHWWHWVIMFVANDFFFYWSHRVS
                     HTVRFMWASHAVHHNSPKLNLSTGMRNSWVGGAMDWVFFAPVVAMGFHPAYLFAIVPI
                     ASGWDYMCHTPYVGRLPGIDFIFNTPSNHRVHHATNERYLDRNLGGMLIIWDRIFGTY
                     AAETEKPTIGALEMPERPNNPINVEFYIWFALLRDAFRRRRTTTT"
     gene            complement(3911..4975)
                     /locus_tag="VMZ53_33635"
     CDS             complement(3911..4975)
                     /locus_tag="VMZ53_33635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HUS33509.1"
                     /translation="MARHAGLEATRTVAIILVVALHAALSFMVTPIGWAIQDRSQFVG
                     VDLIVWVLRAFGMPLFFWLSGFAARALLERRGPSAYLRDRVLRIFLPLAVALLPMSFA
                     LDRLWAWGRDVGGRIAVADVAPKLESSELPIVLGHLWYLYYLLLVSFAALAISRVLRG
                     IKAPPGLAMLAVPSAIAIGVLAYLGALHTNTPLGFVPDIPILVLMGGFFAWGWLVHAR
                     PQEIDRYRDRAWHALAIGGALLAVIIPSLATGRAPLYAIVATGLFTVTMLIVFVGMCV
                     RYARPRAWLQLASEASYWTYIVHLPIVVALQILLARVAIPGVVKYAVILGVTLAVCVG
                     SYVVVVRRLRNASRRSANQM"
BASE COUNT          897 a         1735 c         1666 g          862 t
ORIGIN      
        1 cgatgcgcgg cgcgcggtcg tgtacttcga gtcggtgaac acgagcggct gccatcagtt
       61 cgactccaat tacggcaaca actacccatt cgactgcgcg acgccaccgc aatgggtcgg
      121 caacgcgagc acgctcacga cgcgcgacac gagcggcgac atctgcggcg gtgcgccggc
      181 gaatcagtcg ttcggcttcg acacgtgggt acgccagcgc gcggcgatca cgaacctctg
      241 cttcgaggtc taccaaccgg gcatgaccga ccgcgacgac ccggacatct ggcaaaagct
      301 cgacgtggcg atccactggc gcgaagcgag ctcgctgacg tggcagagcc agcccgtcaa
      361 cttcgatcgc cgcgtcggca acaacgcgcg gttcgcgttc aactggcggc aagtcgatcc
      421 gttccgcttc gcgcactgcc cgacgggaac ggtgacgaca acacccgacg gctacgtcca
      481 agagaccgtc gagtactacg tgacggtgaa cggctaccag ctgcggccag tgccgggcgg
      541 cgcgtacgcg ggcatcttca cggactacaa caacgacgcc tggcgggcag cgaactgcca
      601 ctgattgcgg tcgcatctgg ctttggggtg atgcaagctc gccccgtgcc agagcggctg
      661 ctcaagacga cgacctcgct gtgtcgcgcg tgcaagcgcg cagcgccggc ggagatctgg
      721 cgcatcggca cgaccgtcgt gatgcgcaag cggtgtgagg tgcacggcgc gagtgaggtc
      781 gaagtcgcgt cgaacgcaga ttggtacgag cgcaccgtcg ggtacgcacc ggtgctcgcg
      841 aagcccgagg cgaagaaggc cgtctcgcag ggctgcccgt tcgactgcgg cccgtgcacg
      901 agtcacgagc agcaatcgca gctaccgatc gttccgatca cgagcgcgtg caatctcgat
      961 tgtccgattt gctacacgca caacaagaac ggcgagggcg cgttccacat gacggagacg
     1021 cagctcgccg ccatcctgga gcatctacgc gcggcggatc ccgagcgccg catcatcaat
     1081 ctcacgggtg gcgagccgac gcagcatccg cagttcgagc ggatcgtcga gcgctgcgtc
     1141 gaagaaggca tccatcgcat cacggtgtcg acgcacgggc tgcggtttct caaggacgag
     1201 cgcttgctcg aacggctcgc gaagctcggc gcgcgtatcg tgctgtcatt cgattcgttc
     1261 aagcccgaga cgaaccagca catgctgggc ggcaatttcc tcgacggaaa gctccgcgtg
     1321 ctcgacctgc tcgagaagta tcaagtcgag acgacgctgc tccccgtgct cgcgcgcggc
     1381 gtcaacgacg acgaggtggg cgcattcgta cgactcgcgc tcgagaagcc gttcattcgc
     1441 tcgctcgagc tgcacacgat gacgttcacg ggcgcgaacg ggcagacgtt cgaccggggc
     1501 ggtcgctact cgacgttcga cgtgctcacc gacatcgagg cgcagacggg cggcatgctg
     1561 cgcgtaagcg acttcgtgcc atcgccggca gcgcacccgc tctgctatct cgtgacgtac
     1621 gtgctgaagc tcgacgaagg ccgctggctg ccattcccgc ggttcatgtc gggcgaggac
     1681 ctccgcgagc tgctcgcggg catgctgtat ctcgagccga cgcgcgccac cgaggagaag
     1741 ctcggcgatg tgatcgagcg cctctgggcg ggcgatatcg actgcgatga cagcgagctc
     1801 gtgctctcgc gcttgcgtgc actgacggag agcgtgttcg cgcgcggcgt ggagccggct
     1861 gaacggctgc ggcgcgcgga gatgagcagc aaggcgatct acatccacgc gcacatggac
     1921 gaggacacgt tcgataccga tcgcatccgc caatgctgcg tgggcattcg cgagcccgac
     1981 ggtacgaaca tcccgtcatg cgcgtacaac acgctctacc gcgatcgcga cgcgcggttc
     2041 gccgaaaagc cagcctcgcc gctcgtcacg ctgggcctcg gccgccgcta gccgatgagc
     2101 tcgtcgaggg acgacgttgc cgcgccgccc gacgctgctg ctgcgccgcc gagcacagca
     2161 gctgctgctg cgccgaagca ccggctgtgg tggattctgc tgctggtggt tgtcggaggc
     2221 ggggggattt atctcggcgc gccaaattcc gatgttctac gcgtcgcgtg ggcgcggttg
     2281 tcgtcggttc gcggcgcggc gatcgcgatc gtcgtcggtg gcgcgctcgg tctcgtcgcg
     2341 acagaggcgc tgcgcatcgc ggtgatcggg cggctcgtcg gcgtgcgcgt cagcgcgcgt
     2401 gatgcgtggg atgcggcggt ggcgaatcac gtgatgacgg cggtgacgcc gcaggtcggg
     2461 ctcggcgagc cgacggtcgc gtacacgctc ggcaggcgag gcactccgtg ggacgcggcg
     2521 gtcgcgattc cgttcatcaa gttcacgtcg agcctcgtgc tggtcttcgt tctcggtgcc
     2581 gcgttggtgg gagcgggctt cggccccgtt gtcgacgggc gaatcggcgc gctcggcatc
     2641 gtgtggtttc tcgcgatcgc cgctatcacg tcgctcgtca tcgccatctg ctcgagcccg
     2701 tcgacggcgc ggcgctggat cggtcgcatc gcggggtggt tgtcgcgtcg acgctggttc
     2761 gcgagccccg cgtggcaggc gcgcatcgag aacagcgcgc aggtcacgat tcgcaccgcc
     2821 gaccggctcg cgggcgtgcg acgcgctagc tggcggtcag cgatcgcgct cgtcgcggtc
     2881 cacctcattt actacgcgtc gtacgtcgcg ccgctcgtct gcctcgcgtg cgtcctcggc
     2941 gatccgccgg tcatcacgct cgcgctccgc tcgctcgtct acctctgctt cttgttcgcg
     3001 atgccgacgc ccggtggcac ggggcccggc gaagcggccg ccgccgtctt cttcggcgac
     3061 ctcgtgccgc ccgcggacgc gatcgtcatt gtcgcggtct ttcgagctgc cacgttttac
     3121 ctccaactcg cgattggcgc cctttacctg ccgatccgct cactggcact atcgcgtaga
     3181 tgagtgggct tgattgggtt gcctatatcg cgatgggcgt catgttcgtt gcgttcacgg
     3241 gcgaagtcat cacgagctac ttcatccgca aagacggcta ctacgagaag aacgacacgc
     3301 tcgtcaatct cgccgtcgcg ctcgggtacg tcgtcgcgag catgtcgtac ggcgtgctca
     3361 tcgtcctgat cctcgtgccg gtgtggaagc tcacgccatt ccgctggtcg atggaccact
     3421 ggtggcactg ggtcatcatg ttcgtcgcga acgacttctt cttctactgg tcgcatcgcg
     3481 tgagccacac cgtccgcttc atgtgggcgt cgcacgccgt ccaccacaac tcgccgaagc
     3541 tcaatctctc gacgggaatg cgcaactcgt gggtcggcgg cgcgatggac tgggtgttct
     3601 tcgcgccggt cgtcgcgatg ggctttcacc ccgcatacct gttcgcgatc gtccccatcg
     3661 ccagcggctg ggactacatg tgccatacgc cgtacgtcgg tcgtttgccc ggcatcgact
     3721 tcatcttcaa cacgccgtcg aaccaccgcg tccaccacgc gacgaacgag cgctatctcg
     3781 accgtaacct cggcggcatg ctgatcatct gggaccgcat cttcggcacc tacgccgccg
     3841 aaaccgagaa gcccacgatc ggcgcgctcg agatgccaga gcgcccgaac aacccgatca
     3901 acgtcgagtt ctacatctgg ttcgcgcttc tccgtgacgc gttccgcaga cgccgcacga
     3961 cgacgacgta gctgccaacg cagaccgcga gcgtcacgcc gagaatcacg gcgtacttca
     4021 cgacgcccgg aatcgcgacg cgtgcgagca gaatctgcag cgcgacgaca atcggcagat
     4081 ggacgatgta cgtccaatac gacgcctccg acgccagctg caaccacgca cgaggccgtg
     4141 cataacgcac gcacatgccg acaaagacga tcagcatcgt gacggtgaag aggcccgtcg
     4201 cgacgatcgc gtaaagcggc gcgcgacccg tggcgaggct cggaatgatg acggcgagca
     4261 acgcgcctcc gatcgccagc gcatgccacg cgcgatcccg atagcgatcg atctcctgcg
     4321 gccgtgcatg aacgagccaa ccccacgcaa agaacccgcc catgagcacg aggatcggaa
     4381 tgtcgggcac gaagccgagc ggcgtgttgg tatgcagcgc gcccagatac gcaagcacgc
     4441 cgatcgctat cgccgacgga acggcgagca tcgcgaggcc gggcggtgcc ttgatgccgc
     4501 gcaatacgcg cgagatcgca agcgccgcaa acgacacgag cagcaaatag tagaggtacc
     4561 aaagatgccc gaggacgatg ggcagctccg agctctcgag cttcggcgcg acatccgcca
     4621 cggcgatgcg cccacccacg tcgcggcccc acgcccagag gcgatcgagc gcgaacgaca
     4681 tcggaagcag cgcgacagcg agtggcaaaa agatacgcaa cacgcggtcg cgcaggtacg
     4741 cgctcgggcc acggcgctcg agcaacgcgc gcgctgcgaa tcccgagagc caaaagaaca
     4801 gcggcatgcc aaacgcgcgc aacacccaaa cgatcaaatc gacgccaacg aactgcgacc
     4861 gatcctgaat cgcccaacca atcggcgtga ccatgaagga caacgccgca tgcagcgcga
     4921 ccacgagaat gatcgcgacg gtacgcgtag cttcaagacc cgcgtgccgc gccatctcag
     4981 cccctccgcg ccaccgcgtt cgctacgtcg ctcgtcgtga tgcccgtcgt gccgctcgtc
     5041 gtgccgctcg tcgtgtcgct cgtcgtgtcg ctcgtcgtgt cgctcgtcgt gccgctcgtc
     5101 gtgccgctcg tcgtgtcgct cgtcgtgtcg cgcgtcgcgt cggtcgtcgt tcgcccgcac
//
LOCUS       DATLBZ010000686        10574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8023760, whole genome shotgun sequence.
ACCESSION   DATLBZ010000686 DATLBZ010000000
VERSION     DATLBZ010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8023760"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VMZ53_33640"
     CDS             <1..203
                     /locus_tag="VMZ53_33640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33510.1"
                     /translation="AARWEEDTWEMFAGSGGEIARGDVRVVPVGTLLGNDPSLKRAVE
                     LPIGSAIWRDTGDWKDWDGGRR"
     gene            complement(212..1249)
                     /locus_tag="VMZ53_33645"
     CDS             complement(212..1249)
                     /locus_tag="VMZ53_33645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33511.1"
                     /translation="MRAAAAAVLLAAVLAASSSRAAHAQVSVAVQLNADGEALASQLG
                     ITSQDLASRIQDRVNDAYDAQNVAGFIRSFTDATSFSTRGLGVDYASLPKNLILGVGA
                     NFAVAASGDFNADERPTAGLAANVSFMAGMNLADYDAPRWTLFANGFYRKATLNSLRG
                     GITTAGLHAQYRLLQPQRDEGVTTQVLRWTGMDITSGLEFTRWNLGVDDDIETPFGVD
                     GTSGGANLLLQSTGTFDLSSTAMTVPVEVSTGIRIALLVSVYIGAGVDFTASSGKLTT
                     NLAGDLLTEDNRNIGTTTITGGGNNSGSPVAARVMAGAQLNIWKLRAYVQLNASATPA
                     ASIGAGIRGVL"
     gene            complement(1259..2152)
                     /locus_tag="VMZ53_33650"
     CDS             complement(1259..2152)
                     /locus_tag="VMZ53_33650"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HUS33512.1"
                     /translation="MRGVVLLLALGACNQYFDLTPTELEPGFDSDADGVEDVDDNCRI
                     DVNADQADTDDDGFGDACDPCVAGPQAFKDDDNDGLDNACDPCAGGENSDEDGDTLLD
                     GCDVCPADPNADQADVDADGVGDACDYDFAAPNHRIFFDGFARTLPGWNQTFINWSVA
                     DGTFGPDETSQQFGGPWTRLATIAPSMPWQITAVVDLTTDPPQYGQQVGIIAYTHTGS
                     TAFICGLTYLGAQGWRLAGSGVTVDDVDRITIRSAPGNSGIICVHNGVSIALSAQPTE
                     PFMVGLNSTQRWRFRWLEVLQ"
     gene            complement(2168..3352)
                     /locus_tag="VMZ53_33655"
     CDS             complement(2168..3352)
                     /locus_tag="VMZ53_33655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HUS33513.1"
                     /translation="MKGLLDHMLACEASDLYITAGSPAVFRIDGVGYPAKNPLAPDEI
                     ETMADSLMTPAQQTEFRAKLEMNLALATEDGGRFRANIFRQRGATGMVFRLVRTQIKT
                     LKELNHPPALAELMMQKRGLILVVGGTGSGKSTTLAAMIDHRNRTSSGHIITVEDPVE
                     FIHNHQRCVVTQREVGIDTLSYHHALKNALRQAPDLILIGEIRDAETMEHAITFAETG
                     HLCVATLHANNANQAIERVLNFFPPERAHEIRLQLALNLRAIVSQRLVPTTTGGRAAA
                     LEIMLDTPRVRDLIKRGEIEALKDAMEQGMGEGCKTFDGALFDLILANKLDDAEALKA
                     ADSPNNLRIRIERYRNKGMEAEQPTLRLAPPPAKLKPVAPPAAASTTSSKATGPNGAI
                     RN"
     gene            complement(3394..4458)
                     /locus_tag="VMZ53_33660"
     CDS             complement(3394..4458)
                     /locus_tag="VMZ53_33660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HUS33514.1"
                     /translation="MLTINQLLRFAAQTKASDLHLSAGEPPSLRIHGDIQRVDVPALT
                     PEDTHRLIFDVMNDAQRRIYQEKLEVDFAFALDDELRFRVNAFVQNRGEGAVFRSIPH
                     KIPSFSDLNLPPVLRTFCEQEKGLVLVTGPTGSGKSTTLAAMIDHINETTSGHILTLE
                     DPIEFVHKSKRCLINQREVGTQTHSFNAALKSALREDPDVILVGELRDLETVSLALTA
                     AETGHLVFATVHTSSAPKTVDRLVDVFPTQQQAQIRTMLSESLVGVATQSLLPKIGGG
                     RAAAIEILVATPAVRNLIRENKTFQIPSAMQVGAKSGMVTMEAALMDLVTKGLVNLDD
                     VRHRLPESAEQQRAAQGATR"
     gene            complement(4458..4922)
                     /locus_tag="VMZ53_33665"
     CDS             complement(4458..4922)
                     /locus_tag="VMZ53_33665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33515.1"
                     /translation="MATTSRDDRSQPLLRIPRNQTPVTIMLEDGERASAMLFVAPGTS
                     VMRMLADAAAFVPVSFTSGVRLIARDSIACITVHILHAHVEEFEAVCERQKSLVRLRN
                     GQHMKGELHWIPSSENRRMLDHLNDQSSHLTMHDGDHVSYISKRHVASAEEV"
     gene            complement(4978..7137)
                     /locus_tag="VMZ53_33670"
     CDS             complement(4978..7137)
                     /locus_tag="VMZ53_33670"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUS33516.1"
                     /translation="MTKKFRAGMVGAGNICEFHVAAVKALPDVELIGVCDLDRKRAQE
                     NAEKWGTTAFDSMEQMIEAGANVIHVLTPPAAHAKVAKAALEKGCHVLVEKPIAEDEA
                     EALEVGALARRKGLVASVNHSLLYDPQVKRALDQVKAGKLGEVVSVDILRGSEYPPYE
                     GGNLPPWYRDAGYPFRDIGVHCLYIMQELLGPIEDVEAKWESLGGDPNLAFDEWRALV
                     HCKRGLGQFQLTYNTKPMQSQIIIHGTKGVLRVDLFAMFHGKRASTPLPKAAERLVNA
                     FADSIQPLIDVPVNVFKFVRKEVQAYQGLRDLVRDFYLRLESGEQPPVSVEDAAQVVH
                     WVEKVARDADKEHVQALSKFTLSEKVPYLVTGASGSLGKAVVKKLREQGHRVRVFVRR
                     MPAKPAEGIEYAFGNLGDPVAVDRAVKGAEIVIHCGAAMKGGWPEHKGGTVIGTQNVI
                     DACRKHKVKQLVHISSMSVFDWAGMSGKGTVDESVELEPRADERGAYTRAKLEAERFV
                     SEAATQGLPCVILRPGQIFGGGINLINGAVARNAGGRWLILGDGNLELPLVYIDDVVD
                     SITASIEKKLTKGEVIQIIDPETLTQKDVLDLAGSGKPMLRVPRPVVFALGKLSEIPL
                     GMIGKPSPIGVYRLKSALAKLHYESDRAEKLLGWKPRVGVREGIRRVSQGEAATSTST
                     PSTSRSSDEPSKPAEARPIGRSAAAAPAAPMIAKPKR"
     gene            complement(7134..8294)
                     /locus_tag="VMZ53_33675"
     CDS             complement(7134..8294)
                     /locus_tag="VMZ53_33675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUS33517.1"
                     /translation="MSTPIIAFAKDWHEDPTSNHHVLRELARTRRVLWLNSIATRTPK
                     LSSGRDLGKIRRKLGEFVRGPVNVENDLWVMSPLALPLPGNETASSINRTILRATIRA
                     LRLRLGIDDFQLWTFLPNTAPYVGTLGEKMAIYYCVDEWSMFSYLNRAQTVAAERQLL
                     TKVDAVFAINAALADAKRKLNPRTFVSPHGVDHALFAKALDPATVVPSDIDRIPHPRL
                     GFYGTLRDWVDFELLAHVARERPTWQIVLIGQQLGDLSAIQGLPNVHLLGQKRHDELP
                     AYCRAFDLGMIPYRIDERMTFVNPLKLREYLSAGLPVVSTPVPEVAKFSKLARVATTH
                     EEFVTACEAALKETSEQERRARSDAMKNETWSARVLNVARTIDEMAQRKMNR"
     gene            8322..9839
                     /locus_tag="VMZ53_33680"
     CDS             8322..9839
                     /locus_tag="VMZ53_33680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33518.1"
                     /translation="MPRLAMPPAFFTVFKLCAMATCALSPACKRHVDPGPDFQITTDG
                     VRLRPTDAIPRSSPIFDGNRVTLEAARGETVGFLVYQRTAPQPVTLTGELLPAKLYAV
                     EPAVAKRGSTALYSGGRGVGAYPDILRESSSPATNPAYVELAVPRDAKPGAHAAQLAV
                     GDKHYDVTVNVHDVALPDLPPRVWAYYDPRELQWAKLGAGTHDAPSPEERACIATFRR
                     YGVLLSPDLPLSAWPARRELLAGFPFIPVKLDKDHVADDVRGWIAATKDSGQLPFTIP
                     IDEPRKPDARAKVKALSQQVRDAGGGPSTFLYAVTAEPHAEMGDAIDLYITLQPKLAD
                     TFPRWTYNGAPPRAGSFVLDAVAPGPRTWGWLAWRSKIAVWYVWDALYWHDRHNRKGA
                     PLPGRPLDAPTDATSFDDGEDHGNLDGVLALPGDATTPCMPTLRLAAIRRGQQDRALL
                     ELAEKCHPAEMKALVEKLIPRALGEAPQHGSPAWPTDDAAWEAARVKLLDLATCH"
     gene            complement(9951..10316)
                     /locus_tag="VMZ53_33685"
     CDS             complement(9951..10316)
                     /locus_tag="VMZ53_33685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUS33519.1"
                     /translation="MPTFVAYEVAVELVRELRPMVELIAQHDANLADQMKRAATSVVL
                     NLSEGARRAAGNRRRAYEIAHGEARELLGALDCAAAWGYVLDASRARATLDRLLGLLW
                     GLTHPGHVTRSALRGQTSP"
BASE COUNT         1697 a         3374 c         3603 g         1900 t
ORIGIN      
        1 aggctgcgcg ttgggaggaa gacacgtggg agatgttcgc tggatctggc ggagagatcg
       61 cgaggggcga cgtgcgagtc gtcccggttg gaacactgct cggtaacgat ccttccttga
      121 agcgtgctgt cgagttgccg atcgggtccg caatctggcg cgacacgggg gattggaagg
      181 actgggatgg ggggcgccgc tagagcgcgg gttacagcac gccgcggatg ccagcgccga
      241 tgctcgcagc cggcgtcgct gaggcgttga gctgcacgta ggccctcagc ttccagatgt
      301 tgagctgcgc gccggccatt acgcgagctg ccacggggct gccactgttg ttgccgccac
      361 ccgtgatcgt cgtcgtcccg atgttgcggt tgtcctcggt gagcagatcg ccggcgaggt
      421 tcgtcgtcag cttgccggag ctcgccgtga agtcgacgcc cgcgccgatg tagacgctga
      481 cgagcagcgc gatgcgaatt cccgtcgaaa cctcgacggg gacggtcatc gctgtggatg
      541 acaggtcgaa cgtgccggtc gattgcagca gcaagtttgc gccaccggac gtgccatcga
      601 cgccgaacgg cgtctcgatg tcgtcgtcga cgccgaggtt ccagcgcgtg aactcgaggc
      661 cactcgtgat gtccatgccc gtccagcgca gcacctgcgt ggtgacgccc tcgtcgcgct
      721 gcggctggag gaggcggtat tgggcgtgga ggcccgcggt cgtgatgcca ccgcgcagcg
      781 agttgagcgt cgccttgcgg tagaagccgt tcgcgaagag ggtccagcga ggcgcgtcgt
      841 aatcggcgag gttcatgccc gccatgaacg agacgttggc ggcgaggccg gcggtggggc
      901 gctcgtcggc gttgaagtca ccggaggccg cgaccgcgaa gtttgcgccc acgccgagga
      961 ttaggttttt ggggagggag gcgtagtcga cgccgaggcc gcgcgtcgag aaggaggttg
     1021 cgtcggtgaa ggagcggatg aagccagcga cgttttgcgc gtcgtaggcg tcgttgacgc
     1081 ggtcttggat gcgcgacgcg aggtcctgcg acgtgatgcc gagctgcgat gcgagcgctt
     1141 caccgtcggc gttgagctgg accgcgacgc tgacttgggc atgtgcagcg cgagatgacg
     1201 aagcggcgag caccgcagcg aggagtacgg cggcggcggc tgctctcatc cgcgcactct
     1261 actgcagcac ttcgagccag cggaagcgcc agcgctgcgt gctgttgagg ccgaccatga
     1321 acggctccgt cggctgcgct gagagcgcaa tcgagacgcc gttgtgcacg cagatgatgc
     1381 cgctgttacc gggagcggag cggatggtga tgcgatcgac gtcgtcgacg gtgacgccgg
     1441 agcccgcgag tcgccagcct tgcgcgccca ggtacgtgag gccgcagatg aaggcggtcg
     1501 agcccgtgtg cgtgtacgcg atgatgccga cttgttgacc gtattgcggc ggatccgttg
     1561 tgaggtcgac gaccgccgtg atctgccagg gcattgaggg cgcgatcgtt gcgaggcgcg
     1621 tccagggccc gccaaattgc tgcgaggttt cgtccgggcc gaacgtgccg tcggcgacgg
     1681 accagttgat gaaggtttga ttccagccgg ggagggtgcg agcgaagccg tcgaagaaga
     1741 tgcggtggtt tggggccgcg aagtcgtagt cgcacgcgtc gccgacgccg tcagcgtcca
     1801 catcggcttg gtcggcgttc gggtcggctg ggcagacgtc gcagccgtcg agaagtgtgt
     1861 cgccgtcttc gtcggagttt tcgccgcccg cgcacggatc gcaggcgtta tcgaggccgt
     1921 cgttgtcgtc gtctttgaac gcctgcgggc cggcgacgca tggatcgcac gcgtcaccga
     1981 agccgtcgtc gtcggtgtcg gcttggtcgg cgttgacgtc gatgcgacaa ttgtcgtcga
     2041 cgtcttcgac accgtcggca tcgctatcga agccgggctc gagctcggtg ggcgtgaggt
     2101 cgaagtactg gttacacgcg cctagcgcga gcaacaggac gacgcccctc atgcgtcaga
     2161 ctgtacgtca gtttcggatc gcgccgttgg gaccggtagc cttcgagctc gtcgtcgacg
     2221 cggcggcggg cggggcgacg ggcttgagct tggccggcgg cggggcgagg cgcagcgtcg
     2281 gctgttcggc ttccatgccc ttgttgcggt agcgctcgat gcgaatgcgc aggttgttcg
     2341 ggctgtcggc cgccttgagg gcttcggcgt cgtcgagctt gttcgcgagg atgaggtcga
     2401 agagcgcgcc gtcgaaggtc ttgcagcctt cgcccatgcc ttgctccatc gcgtctttga
     2461 gcgcttcgat ctcgccgcgc ttgatcaggt cgcggacgcg cggggtgtcg agcatgatct
     2521 cgagggctgc tgcgcggccg cccgtcgtcg tcgggacgag gcgctgcgag acgatcgcgc
     2581 ggaggttgag cgcgagctgc aggcggattt cgtgcgcgcg ctccggcggg aagaagttga
     2641 ggacgcgctc gatcgcttgg ttggcgttgt ttgcgtgcag cgtggcgacg cagaggtggc
     2701 cagtctcggc aaacgtgatc gcgtgctcca tcgtttcggc atcgcggatc tcgccgatga
     2761 ggatgaggtc gggagcttgg cggagcgcgt tcttgagcgc gtggtggtac gagagcgtgt
     2821 cgatgccgac ttcgcgttgc gtgacgacgc agcgctggtg gttgtggatg aactcgacgg
     2881 gatcttcgac ggtgatgatg tggccgctgg acgtgcggtt gcggtgatcg atcatcgcgg
     2941 cgagcgtggt cgatttgccg gagcccgtgc caccgacgac gaggatgagg ccgcgcttct
     3001 gcatcatcag ctcggcgagc gccggcgggt gattgagctc tttgagcgtc ttgatctgcg
     3061 tgcggacgag gcggaacacc atgcccgtcg cgccacgctg gcggaagatg ttggcgcgga
     3121 agcgtccgcc gtcttcggtc gcgagcgcga ggttcatctc gagcttcgcg cggaattcgg
     3181 tctgctgcgc cggcgtcatc agcgagtcgg ccatcgtttc gatctcgtcg ggcgcgagcg
     3241 ggttcttcgc cgggtagcca acgccgtcga tgcggaacac cgccggcgag cctgccgtga
     3301 tgtagaggtc ggacgcctcg cacgcgagca tgtgatcgag gagacccttc atcacgggat
     3361 cccaatcgca gtcggggata ctcgttgctc cactcatctt gtggctcctt gtgcggcgcg
     3421 ttgttgttcg gcggattcgg gcaggcggtg acgaacgtcg tcgagattga cgaggccttt
     3481 ggtgaccagg tccatgagcg cggcttccat cgtgaccatg ccggacttcg caccgacctg
     3541 catcgccgac gggatttgga acgtcttgtt ctcgcggatc aggttgcgga cagcgggggt
     3601 cgcgacgagg atttcgatcg ctgctgcgcg gccgccgccg atcttcggaa gaagcgactg
     3661 cgtagcgaca ccgacgagcg actcggagag catcgtgcgg atctgcgctt gctgctgcgt
     3721 cgggaatacg tcgacgaggc ggtcgaccgt cttcggcgcg ctcgaggtgt ggacggtcgc
     3781 gaagaccaag tgacccgtct ccgccgcggt caacgcgagc gaaaccgtct cgagatcgcg
     3841 gagctcgccg acgaggatga cgtcggggtc ttcgcgaagc gcgcttttga gagcggcatt
     3901 gaacgagtgg gtttgcgtgc cgacttcgcg ctggttgatg aggcagcgct tcgacttgtg
     3961 gacgaactcg atcggatctt cgagcgtgag gatgtgaccc gacgtcgtct cgttgatgtg
     4021 atcgatcatc gcggcgagcg tcgtcgactt gcccgagccg gtcgggccgg tcacgaggac
     4081 gaggcctttc tcttgctcgc agaacgtgcg caggaccggc ggcaggttga gatccgagaa
     4141 gctcgggatc ttgtgcggga tcgagcggaa cacggcgcct tcgccgcggt tctgcacgaa
     4201 cgcgttgacg cgaaagcgga gctcgtcatc gagcgcgaac gcgaagtcga cctcgagctt
     4261 ctcttggtag atgcggcgct gcgcgtcgtt catcacgtcg aagatcaggc ggtgcgtgtc
     4321 ttccggcgtg agggcaggga cgtcgacgcg ctggatgtcg ccgtggatgc gcaggctcgg
     4381 cggttcgccc gcggagaggt gcaagtcgct ggctttcgtc tgcgcggcga agcgcaggag
     4441 ctggttgatc gtcaacatca gacttcctcc gccgacgcga cgtggcgctt cgagatgtag
     4501 ctgacgtgat cgccgtcgtg catcgtcaga tgcgagctct gatcgttgag gtgatcgagc
     4561 atgcggcggt tctccgacga cgggatccag tgcagctcgc ccttcatgtg ctggccgttg
     4621 cgcaggcgga cgagcgactt ctggcgctcg cagaccgctt cgaattcctc gacgtgcgcg
     4681 tggaggatgt gcaccgtgat gcaggcgatc gagtcgcgcg cgatcaggcg gacgccgctc
     4741 gtgaacgaga ccggcacgaa tgcagccgcg tcggccagca tgcgcatgac cgacgtgccc
     4801 ggcgcgacga acagcatcgc gctcgcccgc tcgccatctt cgagcatgat ggtgacgggc
     4861 gtctggttgc gcgggatacg gaggagcggc tgcgaacggt cgtctctgct cgttgtcgcc
     4921 atcacctggg tgattcgacc aactcccccc gcaatttacg gaggtctttc gcctacatca
     4981 gcgcttcggc ttggcgatca tcggagccgc gggagcagct gccgcggagc ggccgatcgg
     5041 acgtgcttcc gcaggcttgg aaggctcgtc gctggagcgg ctcgtcgacg gagtcgaggt
     5101 agacgtcgcg gcctcgccct ggcttacccg acgaataccc tcgcggacgc cgacgcgcgg
     5161 cttccagccg aggagcttct cggcgcggtc gctctcgtag tggagcttcg cgagcgccga
     5221 cttcaggcgg tagacgccga tcgggcttgg cttgccgatc atgcccagcg ggatttcgct
     5281 gagcttgccg agcgcgaaga cgaccggacg cggcacgcgc agcatcggct tgcccgaacc
     5341 agcgaggtcg agcacgtcct tctgcgtgag cgtctcgggg tcgatgatct ggatgacctc
     5401 gcccttcgtc agcttcttct cgatcgatgc cgtgatcgag tcgaccacgt catcgatgta
     5461 gacgagcggc agctcgaggt tgccgtcacc gaggatcagc cagcgtccac ccgcgttgcg
     5521 cgcgaccgcg ccgttgatca ggttgatacc gccgccgaag atctgacctg ggcgcaggat
     5581 cacgcacggc aggccctgcg ttgcagcttc gctgacgaag cgctcggctt cgagcttcgc
     5641 acgcgtgtac gcgccacgct cgtcggcgcg gggctcgagc tcgacgctct cgtcgaccgt
     5701 gcctttgccc gacatgccgg cccaatcgaa gacgctcatc gacgagatgt ggacgagctg
     5761 cttcaccttg tgcttgcggc acgcgtcgat cacgttctgc gttccgatga cggtgccgcc
     5821 cttgtgctcg ggccagccac ccttcatcgc ggcgccgcag tggatcacga tctcggcgcc
     5881 cttcacggcg cgatcgacgg ccactggatc accgaggtta ccgaacgcgt actcgatgcc
     5941 ctccgctggc ttcgccggca tgcggcgtac gaacacgcgc acgcgatgac cttgctcgcg
     6001 cagcttcttc acgacggcct tgccgagcga gcccgacgcg ccggtgacga ggtacgggac
     6061 cttctcggag agcgtgaact tcgagagcgc ctggacgtgc tccttgtcgg cgtcacgggc
     6121 gaccttctcg acccagtgca cgacctgtgc ggcatcctcg acggagaccg gcggctgctc
     6181 accggactcg aggcgcagat agaaatcgcg gacgaggtcg cgcagacctt gatacgcctg
     6241 gacttccttg cgcacgaact tgaagacgtt caccggaacg tcgatcagcg gctggatcga
     6301 atccgcgaac gcgttgacga gacgctcggc ggccttcgga agcggcgtcg aagcgcgctt
     6361 gccgtggaac atcgcgaaca gatccacgcg cagcacgccc ttcgtgccgt ggatgatgat
     6421 ctgcgactgc atcggcttcg tgttgtaggt cagctggaac tgaccgaggc cacgcttgca
     6481 gtgaacgagc gcgcgccact cgtcgaacgc gaggttcgga tcgccaccga gcgattccca
     6541 cttcgcttcg acgtcttcga tcgggccgag cagctcttgc atgatgtaga ggcagtggac
     6601 gccgatgtcg cggaacggat agcccgcgtc gcggtaccac ggcggcaggt ttccaccttc
     6661 gtacggcgga tactccgagc cgcgcaggat gtcgacggag acgacctcgc cgagcttgcc
     6721 ggccttcacc tgatcgagcg cgcgcttgac ctgcggatcg tagagcagcg agtggttcac
     6781 cgacgcgacg agacccttgc gccgcgcgag tgcaccgact tcgagcgcct cggcttcgtc
     6841 ctctgcaatc ggtttctcga cgaggacgtg gcagcccttc tcgagcgcag ccttcgcgac
     6901 cttcgcgtgc gcggccggcg gcgtgagcac gtggatgacg ttggcacccg cctcgatcat
     6961 ttgctccatc gagtcgaacg cagtggtgcc ccacttctcg gcgttctctt gcgcacgctt
     7021 gcgatcgagg tcgcaaacgc cgatcagctc gacgtcgggc agtgccttca cggccgcgac
     7081 gtggaactcg cagatgttgc ctgcgccgac catgcctgct cgaaactttt tcgtcatcgg
     7141 ttcatcttcc tttgcgccat ctcgtcgatc gtgcgcgcca cattgaggac tcgcgccgac
     7201 caggtttcgt tcttcatcgc atccgaccgc gctctgcgct cttgctcgct cgtctctttc
     7261 agtgctgctt cacacgccgt cacaaactct tcgtgcgtcg tcgcgacgcg cgcgagcttc
     7321 gaaaacttcg ccacctccgg taccggtgtc gacacgacgg ggaggcccgc cgacaggtac
     7381 tcgcgcagct tgagcggatt gacgaaggtc attcgctcgt cgatgcgata ggggatcatg
     7441 ccgaggtcga acgctctgca ataagcgggc aactcgtcgt ggcgcttctg ccccagaagg
     7501 tgaacgttag gtagcccttg gatcgccgat agatcgccta gttgctggcc aatcaagacg
     7561 atctgccaag tggggcgttc gcgcgcgacg tgcgcgagaa gctcgaaatc gacccaatca
     7621 cgcagcgtcc cgtagaaccc caaacgcggg tgcggaatgc ggtcgatgtc ggatggtacg
     7681 accgtcgccg gatcgagagc cttcgcgaac agcgcatgat cgacgccgtg cggtgacacg
     7741 aacgtgcgcg gattgagctt gcgcttcgca tcggcgagcg ccgcattgat cgcaaagacc
     7801 gcatcgactt tcgtcagcag ctggcgttcc gcggcgacgg tctgcgcgcg attgaggtag
     7861 ctgaacatcg accattcgtc gacgcagtaa tagatcgcca tcttctcgcc gagcgtgccg
     7921 acgtacggcg ccgtattcgg cagaaacgtc cacagctgaa agtcatcgat gccgaggcgc
     7981 aggcgcagcg cacgaatcgt cgcgcgcaag atcgtccggt tgatcgagct cgccgtctcg
     8041 ttgccaggca gcggcagagc aagcggcgac atgacccaca aatcgttctc gacattcacg
     8101 ggcccgcgca cgaactcgcc gagcttgcgc cggatcttcc cgagatcacg gccgctcgag
     8161 agctttggcg tacgagttgc gatcgagttc agccacagca cacgccgcgt tctggcaagc
     8221 tcgcgaagca cgtgatggtt cgaggttgga tcctcgtgcc agtccttcgc gaaggcgatg
     8281 ataggtgtgc tcacgtgtcg acggaaaacg gtactcgcct catgcctcgc ctcgcaatgc
     8341 cgccggcctt cttcacggtc ttcaagctgt gcgcgatggc cacgtgtgcg ctttcgcccg
     8401 cgtgcaaacg gcacgtcgat ccgggccccg acttccagat cacgaccgac ggtgtacgcc
     8461 tgcgtccgac cgacgccatc ccgcgctcat ccccaatctt cgacggtaac cgcgtcacgc
     8521 tcgaagccgc ccgcggcgag accgtcggct ttctcgtcta ccagcgcacc gctccacagc
     8581 ccgtcacgct gaccggcgag ctactcccgg cgaagctcta cgcggtcgag ccggcggtgg
     8641 caaagcgcgg gtcgacagcg ctctacagcg gcggtcgcgg cgtgggcgcg taccccgaca
     8701 tcctgcgcga atcctcatcg ccggcaacaa accctgcata cgtcgagctc gcggtcccac
     8761 gcgacgccaa gcctggcgct cacgccgcgc agctcgcggt cggcgacaaa cactacgacg
     8821 tcacggtcaa cgtccacgac gtcgcgctcc ccgacctgcc accccgcgtc tgggcctact
     8881 acgatccgcg cgagctgcag tgggcgaagc tcggcgcagg cacgcacgac gcgccctccc
     8941 ccgaagagcg cgcctgcatc gcgacgttcc gccgctacgg cgttctcctc tcgccagacc
     9001 tcccgctctc ggcgtggccc gcacgtcgcg agctgcttgc cggcttcccc ttcatcccgg
     9061 tcaagctcga caaagaccac gtcgccgacg acgttcgcgg ctggatcgcc gcaaccaaag
     9121 actccggtca gctgccgttc accattccga tcgacgaacc gcgcaagccc gacgcacgcg
     9181 caaaagtcaa agcgctctcg cagcaagtgc gcgacgcggg tggtggcccg tcgacgtttc
     9241 tctacgcggt gaccgccgag ccgcacgccg aaatgggcga cgcgatcgat ctctacatca
     9301 cgctgcaacc gaagctcgcc gacaccttcc ctcgctggac gtacaacggc gcgccgcccc
     9361 gcgctggatc gttcgtgctc gacgccgtcg cgcccggacc acggacgtgg ggatggctcg
     9421 cgtggcgctc gaagatcgcg gtctggtacg tgtgggacgc gctgtactgg cacgaccgtc
     9481 acaatcgcaa aggcgcgccg cttcccggtc gcccgctcga cgcaccgacg gacgcgacca
     9541 gcttcgacga cggcgaggat cacggaaacc tcgacggtgt gctcgcgctt cccggcgacg
     9601 cgacgacgcc atgcatgcca acactgcgcc tcgccgcgat tcgccgaggc caacaagatc
     9661 gcgcgctgct ggagctcgcg gagaagtgcc accccgccga aatgaaggcg ctcgtcgaga
     9721 agttgatccc gcgcgcgctc ggcgaagcgc cgcaacacgg ctcgccggca tggccgaccg
     9781 acgacgctgc gtgggaagcg gcgcgcgtga agctgctcga cctcgcaacg tgccactgac
     9841 gccgcgaaca tcaagcaacg cgtgcccacg tgggcacgag ctcatcgcac gaggaagcca
     9901 cgcgctgtgc acgtgggcgc aagctcgccg ccaggtctac tgcccgctgg ttagggcgac
     9961 gtctgcccac gtagggccga gcgtgttaca tgacccgggt gcgtgaggcc ccacagtaga
    10021 ccgagcaggc gatcgagcgt ggcgcgcgcg cgcgacgcat cgagcacata gccccaggca
    10081 gcagcgcagt cgagcgcacc gagtagctcg cgcgcttcgc cgtgcgcgat ctcgtacgcg
    10141 cgccgacgat tgcctgcggc gcgcctcgcg ccctcgctga gattcagcac cacgctggtc
    10201 gccgcgcgct tcatctgatc cgccaggttc gcgtcgtgct gagcgatgag ctcgaccatg
    10261 ggacgcagct ctcgaacgag ctcgacggca acttcatacg caacaaacgt gggcataccg
    10321 actctccttg gtgacgcgaa agcacccgaa cggaaccacc tctcttgtct ccccttgggg
    10381 ctggggggcc tcccggaggt gggggcgggg cggcggagcg gggacgctct tcgccgttcg
    10441 cgcgagtgcg ccggaggcgt gacgagtgct ctcccggccc gcacgcgatc tcccgaacgc
    10501 gcgtctccgc ggcgacggct cgcgatcccc cgagcgcttc ggcactcggc gcgcctctgg
    10561 cgcaaacgag cgcg
//
LOCUS       DATLBZ010000687         4637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6525907, whole genome shotgun sequence.
ACCESSION   DATLBZ010000687 DATLBZ010000000
VERSION     DATLBZ010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6525907"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VMZ53_33690"
     CDS             <1..481
                     /locus_tag="VMZ53_33690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33520.1"
                     /translation="FTVAAVEHRSVAAALDELPLYIQRGAIVPMLRDTIDTLAPATVS
                     GIESYANDPGLLVVRIAPGPHSAFTLFDETKIEQQLEAGVTTVDYTPGKTFVGGALLE
                     SIATDAPAAVTTVSGALTQRASYAALQAAADGWFYDATATGGTLWIKLAGAATVTVR"
     gene            696..1868
                     /locus_tag="VMZ53_33695"
     CDS             696..1868
                     /locus_tag="VMZ53_33695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33521.1"
                     /translation="MRLAVGCVVALVGALVAEPVRRADATPAARQSRQTAAAAAAAAA
                     AAALAITAQDLIDVANGDEQPEAVTFGISSKDQVLAIHRLLRAGAASCKRRDDGACGA
                     GVYDAPGVKDSEKSPCFRRWELFTLLYVVAGDVLHLDASEMKAVVKLDDELVQRVGEL
                     LDSAQQVELARSLAKLNRVPPFRLMIDPDEAVKAVKRYHLDAAVEIIEPQQTPEVLIG
                     VVLDPTYKARTRIDALHRLRDETELDLARVARPLLADPSIELAALAASITDTAITRPA
                     TTDRSVFMHALSLFVQADTTDAAERSVTFAPRGLDIVDPTTGEVTDHYGPLARARPNK
                     LADSEELDYQIRQRLRYQPPIFATDAQGFLVIVGFLDVDEPPLPSDTCELLEMGGY"
     gene            complement(1874..2515)
                     /locus_tag="VMZ53_33700"
     CDS             complement(1874..2515)
                     /locus_tag="VMZ53_33700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33522.1"
                     /translation="MLHAARFGRALPEPPLAFEPQPEADTADDLACAATPRSPEAIQR
                     LARCILTGTMRTKYWIAWAGVAIGLWVLGTAVALVITKLVPAWGSTPILALGFGGLGG
                     GLALGALWIRKRRREAQTIAREGQLFTGSVTYRRAQESFGGELSEMLALPLGETYYCI
                     VFAIGFIAHEVWVALPSPPAEGEHHHVLVQSGARLALAFDAHGHGHVGELRRL"
     gene            complement(2635..3087)
                     /locus_tag="VMZ53_33705"
     CDS             complement(2635..3087)
                     /locus_tag="VMZ53_33705"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HUS33523.1"
                     /translation="MTARIFQLAASQGGVPKLPLREARVTTLGLDVDKQKHKKFHGGP
                     DRALCLFSVEVIEALQAEGHPIYPGSTGENVLISGLDWTKLAEGTRIALGDEVIVALT
                     RTTTPCKQIADSFIGRKFQRLAALHEMRWYCRVEREGLLRVAMPVSLL"
     gene            complement(3084..3713)
                     /locus_tag="VMZ53_33710"
     CDS             complement(3084..3713)
                     /locus_tag="VMZ53_33710"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUS33524.1"
                     /translation="MSLTLYVGSKRLSSWSLRPYLALSEAGATFETVVILLDQDDTKA
                     KIGAVNPAGRVPVLHDDGHVIWDSLAICEYANELFPHAKLWPADRTARAHARSISAEM
                     HSGFAALRREMPMDLIAKKPGVGHTPEALADARRVMHIWRECLSSNGPFLFGHFTIAD
                     AMFAPVTTRFTTYGVELDAPCRAYVDMIQSMPSFKAWLADAAKEPQTRP"
     gene            3744..>4637
                     /locus_tag="VMZ53_33715"
     CDS             3744..>4637
                     /locus_tag="VMZ53_33715"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HUS33525.1"
                     /translation="MYKSTPVVAAQRLVNQHLAAPILNAESLVTAMGAIQAQEYWGGL
                     WAIGLRTRGTDAASIEASLVRGSILRTHPMRGTHHFVARGDIRWLIDLMGPLMIKRNE
                     RRNRDLKLDEKTLRKALTAIERALAGGTHLTRTEVAEVLHRAKISTDGQRLAHIIYRA
                     ELEGLVCSGARKGKQVTIALLSERAPDAKPKSREASLRELATRYFATRGPATRADFTW
                     WCQLPAADVREAMELAKLDSDGTYFTKGTRPNAKPPRALLLPPYDEYTVAYRDRSAAG
                     TPPTKAATFGESTLLGPNIVVD"
BASE COUNT          792 a         1572 c         1540 g          733 t
ORIGIN      
        1 attcacggtc gccgcggtcg agcaccgctc cgtcgccgcc gctctcgacg agctgccgct
       61 gtacatccaa cgcggcgcca tcgtgccgat gctgcgcgac acgatcgaca cactcgctcc
      121 ggccacggtc tcgggcatcg agtcgtacgc gaatgatcca ggcctgcttg tcgtgcgcat
      181 cgcgccggga ccccacagcg cgttcacgct gttcgacgaa acgaagatcg agcagcagct
      241 cgaagcggga gtcacgacgg tcgactacac gccgggcaag acgttcgtcg gtggcgcgct
      301 gctcgagtcg atcgcgaccg atgcgccggc cgccgtgacc acggtatcgg gcgcgctaac
      361 gcaacgcgca agctacgccg ccctgcaggc cgcggccgac ggttggttct acgacgcgac
      421 ggccacgggc ggaaccctgt ggatcaagct cgccggcgcc gccaccgtta ccgtccgctg
      481 aagccgccgc actgccagct cgctacggcg gagtggccgc gcggcggacg cgaacactta
      541 ccccggtgac gcgaacactt accccggtac gtgtttggcg ccgtaagtag ctgcgagctc
      601 gtcgtgaaaa cttaccccgg tcggtttcgg cgtcccggga aacttacccc ggttgtttcc
      661 ggcaacccga ggaggccaac gggtgtccgg tggtcgtgcg tctcgcggtc ggttgtgtcg
      721 tcgcgctcgt cggcgcgttg gtcgcggagc ctgtccgtcg ggcggacgca acgccggcgg
      781 cgcggcagtc gagacagacg gcggcggcgg cggcggcggc ggcggcggcg gcggcgctgg
      841 cgatcacggc gcaggatctg atcgacgtcg cgaacggcga tgagcagccg gaggccgtga
      901 catttggcat ctcgagcaag gaccaggtcc tcgcgatcca tcggctgctt cgcgcgggcg
      961 cggcgagctg caagcgtcgc gacgacggcg cctgtggtgc tggggtctat gacgcgcccg
     1021 gcgtgaaaga ctcggagaag tcgccgtgct ttcggcggtg ggagctgttc acgctgttgt
     1081 atgtcgtagc gggcgacgtt ctgcatctcg atgcgagcga gatgaaggcc gtcgtgaagc
     1141 tcgatgacga gctggtgcag cgcgtgggcg agctgctcga ttcggcgcag caggtcgagc
     1201 tcgcgcggag cttggcgaag ctgaatcgcg tgccgccatt tcgactcatg atcgaccccg
     1261 atgaggcggt gaaggctgtg aagcgctacc acctcgacgc cgcggtcgag atcatcgagc
     1321 cgcagcaaac gccggaagtg ctcatcggtg tcgtgctcga tccgacgtac aaggcgcgaa
     1381 cccgtatcga tgcgctgcac cggttgcgcg acgagaccga gctcgatctc gcgcgcgttg
     1441 cgaggccgct ccttgccgat ccgtcgatcg agcttgcggc attggcggca tcgatcaccg
     1501 acaccgcgat cacgcgaccc gcgacgaccg atcgcagcgt cttcatgcac gcgctctcgc
     1561 tgttcgtgca ggcggacacg accgacgcag ccgagcgctc tgttacgttc gcgccgcgcg
     1621 gcctcgacat tgtcgatccg acgacgggtg aggtcaccga tcactacggc ccactcgcgc
     1681 gcgcgcgtcc gaacaagctc gcggattccg aagagctcga ctatcaaatc cggcagcgcc
     1741 tgcgctatca gccgccgatc ttcgcgaccg atgcgcaggg ctttctcgtc attgttggct
     1801 tcctcgacgt cgacgagccg ccgctgccgt ccgatacgtg cgagctgctg gagatgggcg
     1861 gctactagga ggcctagagg cggcgcagct cgccgacgtg accgtggcca tgcgcgtcga
     1921 atgcgagcgc gaggcgggca cctgactgca cgagcacgtg atgatgctcg ccttcggccg
     1981 gaggcgacgg cagcgcgacc cacacttcat gcgcgatgaa gccgatcgcg aacacgatgc
     2041 agtagtacgt ctcgccgagc ggcagcgcga gcatctcgct cagctcgccg ccgaacgact
     2101 cttgcgcgcg gcgatacgtg acgctgcccg tgaacagctg gccttcgcgc gcgatcgtct
     2161 gtgcttcgcg acggcgcttg cggatccaaa gcgcgccgag cgcgagaccg ccaccgaggc
     2221 caccgaatcc gagcgcgagg atcggcgtgc tgccccacgc gggcacgagc ttcgtgatca
     2281 cgagcgcgac ggcggtgccg agcacccaca ggccgatcgc gacgcccgcc cacgcgatcc
     2341 aatacttcgt gcgcatcgtg ccggtcagaa tgcatcgcgc gaggcgctgg attgcttccg
     2401 gcgaacgcgg cgttgctgca cacgcaagat catctgccgt gtccgcctcg ggctgcggct
     2461 cgaacgccag cggcggctcc ggaagagcgc gaccgaaccg agcggcgtgc aacatcgccg
     2521 atcaactacc gcagcgccga gcgcgcttga agtggcgacc ggagttgccg tcggcgcgag
     2581 ccatcgaacc tcttgaaatc accgaagcca cgcggattcg ccgcgcgaga attttcagag
     2641 caggctgacc ggcatcgcga cgcggagtag gccctcgcgc tcgacgcggc agtaccagcg
     2701 catctcgtgt agggcggcga gtcgttggaa cttccggccg atgaaggagt ccgcgatctg
     2761 cttgcatggc gtcgtcgtgc gcgtcagcgc gacgatcact tcgtcaccga gcgcgatgcg
     2821 agtgccctcc gcgagcttcg tccagtcgag accgctgatc aggacgttct cgcccgtcga
     2881 gcccggatag atcggatggc cctcggcctg cagcgcttcg atcacctcga ccgagaacag
     2941 gcacagcgcg cggtcggggc cgccgtgaaa cttcttgtgc ttctgcttgt cgacgtcgag
     3001 gccgagcgtc gtcactcgtg cttcgcgcag cggcagcttc ggcactccgc cctgcgacgc
     3061 ggcgagctga aagatgcgcg cggtcacggc cgggtctgcg gctctttcgc ggcatcggcg
     3121 agccacgcct tgaacgacgg catgctctgg atcatatcga cgtacgcgcg gcacggcgca
     3181 tcgagctcga cgccgtacgt cgtgaaccgc gtcgtcaccg gcgcgaacat cgcatctgca
     3241 atcgtgaagt gaccgaacag gaagggcccg ttgctcgaca ggcactcgcg ccagatgtgc
     3301 atcacgcgtc gcgcatcggc gagcgcctct ggcgtgtgac cgacaccagg cttcttcgcg
     3361 atcagatcca tcggcatctc gcggcggagc gcggcgaacc cagagtgcat ctccgcgctg
     3421 atcgacctcg cgtgcgcacg cgccgtgcga tcggccggcc acagcttcgc gtgcggaaac
     3481 agctcgttcg catactcgca gatcgcgagc gaatcccaga tgacgtgccc gtcgtcgtgc
     3541 agcaccggca cccggccggc cggattcact gcgccgatct tcgccttggt gtcgtcctgg
     3601 tcgagcagga tcacgacggt ctcgaacgtc gcgcccgcct cggagagcgc gagatacgga
     3661 cgcagcgacc acgacgagag gcgcttactt ccgacgtaga gcgtgagcga catgcgcggc
     3721 actctagcaa ctcggcgagc gtcatgtaca agtcgacgcc cgtggtggca gcacagcgtc
     3781 tcgtgaatca gcatctcgcc gcgccgattc tcaatgcgga gagcctcgtg accgcgatgg
     3841 gcgccatcca ggcgcaggag tattggggcg ggctgtgggc gatcggcctg cgcacgcgcg
     3901 gcaccgatgc agcctcgatc gaagcgagcc tcgtgcgcgg cagcatcctg cgcacgcatc
     3961 cgatgcgcgg cacgcatcac ttcgtcgcgc gcggcgacat ccgctggctc atcgacctca
     4021 tggggccgct catgatcaag cgcaacgagc gccgcaatcg cgacctgaag ctcgacgaga
     4081 agacgctgcg caaagcgctc acagccatcg agcgtgcgct cgccggcggg actcacctca
     4141 cacgcaccga agttgccgag gttctccacc gcgcgaagat ctcgaccgac ggccaacgcc
     4201 tcgcacacat catctatcgc gcagagctcg aagggctcgt gtgcagcggt gcgcgcaagg
     4261 gtaagcaggt cacgatcgcg ctgctctccg aacgcgcgcc cgatgcgaaa ccgaaatcgc
     4321 gcgaggcgtc tctgcgcgag ctcgcaacgc gctacttcgc gacgcgtggt cccgcaacgc
     4381 gcgccgactt cacgtggtgg tgtcagcttc ccgccgccga tgttcgcgaa gcgatggagc
     4441 tcgcgaagct cgacagcgac ggcacatact tcacgaaggg aacgcggccg aacgcgaaac
     4501 caccacgcgc gttgctgctt cctccgtacg acgagtacac cgtcgcgtat cgcgatcgat
     4561 cggctgccgg tacgccaccg acgaaagccg ccacgttcgg cgagtcaaca ctgcttggac
     4621 cgaacatcgt cgtggac
//
LOCUS       DATLBZ010000688         6044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8028979, whole genome shotgun sequence.
ACCESSION   DATLBZ010000688 DATLBZ010000000
VERSION     DATLBZ010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8028979"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VMZ53_33720"
     CDS             complement(<1..609)
                     /locus_tag="VMZ53_33720"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HUS33526.1"
                     /translation="MRYAVLLAVFLTGAAVARADTVVIAPTQDTTIYSEDGALSNGKG
                     QHLFAGTTSSQSKQRRALIQFGVTGAIPAGSTINSVTLTMRLDKTQANTAAIALYKVT
                     AAWGEGASTATGEEGGGIAAAAGDATWTMRSSPSVAWTSAGGDAVATASATTSVGSAM
                     VDYTWSGAAMVADVQSWLDAPAGNYGWLVRAPTVVAGQAKRFA"
     gene            767..1654
                     /locus_tag="VMZ53_33725"
     CDS             767..1654
                     /locus_tag="VMZ53_33725"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS33527.1"
                     /translation="MVRKPRILAVDDTPSNLIALEAVLDREFDLRYARSGQEAIASLT
                     EDHDVDVILMDVQMPGMDGFEAAGLIKQIPGCLETPIVFVTAVYTEDPHLKRGYQVGG
                     VDYFTKPFDPDLLRLKVGIYASFRQRAALLKERELQIRATEELFTAGKKLSGLLESLP
                     VGVLIADVDGRICQSNDEVSRICKSAELIKNDEYGKMLGWWDASGRLFKAGGGPLSRA
                     LHAKESQTEMMQIQCVDGTTKAVLTSASPLFGLDGHIVGAVIVIQDVSEPKRIGAELQ
                     HRIARLVSLGVELEESIQH"
     gene            1689..2453
                     /locus_tag="VMZ53_33730"
     CDS             1689..2453
                     /locus_tag="VMZ53_33730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33528.1"
                     /translation="MSLEFVDLETAKTATGTRIVVSSLVPSPWSEALKGLLHVAGIRT
                     LVVRRGTDREEIDRWTGIDNVPALFHDREPIRTNWAAITAFVDRQAPTAKLLPATVAA
                     RADVIGALEVIAGEGGIGWNARLVMIDAGITTEGGTGFPLPVAKSLARRYGYSPEGVE
                     LAKARMPEQLAFVASKLAGNYFGGSAPNALDVYSATFLTPLAGPISEQECPGMLPPLR
                     VAFATAHSTFGALIPPALLAHRERMFERHLIRPIQL"
     gene            2460..3668
                     /locus_tag="VMZ53_33735"
     CDS             2460..3668
                     /locus_tag="VMZ53_33735"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS33529.1"
                     /translation="MRVVFLSPRYPPEMREFTRGLAEVGATVLGVGDGHPDDELKRYL
                     SDYLEVPSLFDEADVIRRVHAWLRGRSVDRILANWEPLVITAARLREMFGMPGMSVDA
                     VRGFRDKQLMKERVAAAGIRVPRSARAHTLAEVRVALEATGYPAIVKPISGAGSADTY
                     RVDSERELDTIAPLLRHVDEVSVEEFITGEELTYDTVCIGGKPVYESVTQYIPNALEM
                     RSQEWVSPIMLSVRDLSQPNIQTGIALGRRVLDALGMGDGMTHMEWFRKPDGDVVFGE
                     IACRPGGACVVDLMNYTSDIDLFREWARAVCWHAFEAPTDRKYNVGIVFKRAHGQGRI
                     TTIAGLDDYYRRFGRHIVADTLLRPGTPRRDWKQTLLSDGYLVVRHPDWDTAREMTFA
                     AATGVQLYAS"
     gene            3680..4927
                     /locus_tag="VMZ53_33740"
     CDS             3680..4927
                     /locus_tag="VMZ53_33740"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUS33530.1"
                     /translation="MRHIVFIAPRFFDNTNRFLQGFVELDNITLSVISLDPESAIPES
                     MHPKIAGHYGVKSLDTSELTTAARWLATSIGPIDRLVGFLEELQVPLAEVRTALDIEG
                     LRTQAAHNFRDKDQMKTVLRAAGVPVARSTLATTRAELEAFIADVGYPVIVKPRAGLG
                     TRATYRVGERAELDALPAPSPAAPLQVEEFVGAREHTCETVTIRGKPIWRSGTRYFPS
                     PLEVLDTPWIQYAVLLPREADDPTWTNFHPTNTAALAALFGPDATTSAAGTALTHMEW
                     FMRDDGTSLVNEVGVRPPGVHIMPMMGLTHDTDMFADWARLMALEEFTPKPRVCAAGS
                     AFFRGPAPRQTRGARIVSVTGIERAVEECGDSLVILRTPKIGQPRSEHYEGEGWAIVK
                     HATTEGVKNALRTLIENVQIRYG"
     gene            4978..5379
                     /locus_tag="VMZ53_33745"
     CDS             4978..5379
                     /locus_tag="VMZ53_33745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS33531.1"
                     /translation="MTNDDHSDGLLTGISILVLEDDRDTLDLFAGSLTKLGGTVRKAS
                     TVAAALTTLATWRPNVILCDLHMPETDGYAFLASLRAAEILIPVIAISASHPTVERDR
                     ALEAGFAEYLVKPSRIAETALVIKKIAKPRA"
     gene            complement(5381..>6044)
                     /locus_tag="VMZ53_33750"
     CDS             complement(5381..>6044)
                     /locus_tag="VMZ53_33750"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUS33532.1"
                     /translation="NPMTALSFVETMRAEGHKAIVHTAAASNLGQMLVKICKADGVPL
                     VNIVRKPAHVEQLRALGAEHVLDSSSSTFAAELVEAVAATGATIAFDAIGGGSLGGRI
                     LDAMERVASRSMSTFSHYGSSTLKQLYIYGSLDRAPTTLSRTFGLTWSVGGYLVMNAM
                     RKLGPEAVGRMFKRVASELRTTFASHYTARITLDQLLDLETLRACAQMATGEKYLIVS
                     SR"
BASE COUNT         1091 a         2187 c         1782 g          984 t
ORIGIN      
        1 cgcaaatcgc ttcgcttgtc cggcgaccac cgtcggtgcg cgcacgagcc aaccgtagtt
       61 cccggcgggc gcatcgagcc agctctgcac gtcggcgacc atcgccgcgc cgctccacgt
      121 gtagtcgacc attgcgctgc cgacgctcgt cgtcgcactc gcggtcgcga cggcgtcgcc
      181 gcccgcgctc gtccatgcca ccgacggcga gctgcgcatc gtccacgtcg cgtcacccgc
      241 ggccgccgca ataccgccgc cctcctcgcc ggtcgccgtc gacgcaccct cgccccacgc
      301 tgcagtgacc ttatagagcg cgatcgccgc ggtgttcgcc tgcgtcttgt cgagacgcat
      361 cgtcagcgtg acgctgttga tcgtcgagcc cgcaggaatc gcgccggtga cgccaaactg
      421 gatcagcgcg cgacgttgct tgctctgact gctcgtcgtt ccggcaaaca ggtgctgccc
      481 ctttccgttc gagagcgcac cgtcctcgct gtagatcgtc gtgtcctgcg tcggcgcgat
      541 cacgaccgta tcggcgcgcg caaccgctgc tcccgtaagg aagacggcga ggagtactgc
      601 gtaccgcatc ggccgtagag cgtagcgccg cctaacgggg ccccgggagc gtctcactga
      661 gttctaggga acgaagaatc actagaagat cgtcctcggg aggtgcgcga tcacgcgcgg
      721 ttgccagttg cgcgatgaat cgcatacttc gagtttggga gggtacatgg ttcgcaaacc
      781 aaggattctt gctgtcgacg acaccccttc caacctgatc gcgctggagg cggtcctcga
      841 ccgcgaattc gatctgcggt acgcgcgctc cggtcaggaa gcgatcgcaa gcctcacaga
      901 agatcacgac gtcgacgtga tcctcatgga cgtccaaatg ccgggcatgg acggcttcga
      961 agcggcaggc ctgatcaaac aaatccccgg ctgcctcgaa accccgatcg tgttcgtcac
     1021 cgccgtctac acggaagacc cgcacttgaa gcgcggctat caagtcggcg gcgtcgacta
     1081 cttcacgaag ccgttcgacc ccgacctcct ccgcctcaaa gtcgggattt acgcgtcgtt
     1141 ccgccaacgc gccgcgctgc tcaaagaacg cgagctccag atccgcgcca ccgaagagct
     1201 gtttaccgcc ggcaaaaagc tatccggcct cctcgaaagc ctcccggtgg gcgttctgat
     1261 cgccgacgtc gacggacgta tctgccagtc gaacgacgaa gtctcgcgga tctgcaaatc
     1321 cgccgagctg atcaagaacg acgagtacgg aaagatgctg ggttggtggg acgcgagtgg
     1381 ccgcctcttc aaggccggcg gtgggccgct gtcgcgcgcg ctccacgcca aagagagcca
     1441 gaccgaaatg atgcagatcc aatgcgtcga tggcacgacg aaagccgtcc tgacatccgc
     1501 ctcaccgttg ttcggcctcg acggtcacat cgtcggagcc gtcatcgtga tccaagacgt
     1561 cagcgagcca aagcgcatcg gcgcggagct ccagcaccgg atcgcacgcc tcgtctcact
     1621 cggcgtcgag ctcgaagaga gcatccaaca ctgaaccgca acgtcgcgcg acacgatact
     1681 ctcgcggggt gagcctcgag tttgtagacc tcgaaactgc caagaccgcg accggcactc
     1741 gcatcgttgt ctcgtcgctc gtcccgagcc cgtggtccga agcgctcaaa ggcctgctcc
     1801 acgtcgcggg cattcgcacc ctcgtcgtgc ggcgcggtac cgaccgcgaa gagatcgatc
     1861 ggtggaccgg catcgacaac gtgcctgccc tgttccacga ccgcgagccc attcgcacga
     1921 actgggcagc catcacggca ttcgtcgatc gccaagctcc caccgcgaag ctgctgcccg
     1981 ccaccgtcgc cgcacgcgcg gacgtcatcg gtgcgctcga ggtgatcgca ggggaaggcg
     2041 gtatcggctg gaacgcacgc ctcgtcatga tcgacgccgg cattacgacc gagggtggca
     2101 cgggctttcc gctgcccgtc gcgaagagcc tcgcgcgccg ctatggctac tcgcccgaag
     2161 gcgtcgagct cgcgaaagcc cgtatgccgg agcagctcgc gttcgtcgca tccaagctcg
     2221 ccggcaacta cttcggcggc agcgcgccga acgccctcga cgtctacagc gcgacgtttc
     2281 tgacgccact cgccggaccg atcagcgagc aagagtgccc aggcatgctg ccgccactgc
     2341 gcgtcgcgtt tgccaccgcg cactcgacgt tcggcgcgct catcccaccg gctctgctcg
     2401 cgcatcgcga acggatgttc gaacgccatc tcatccggcc catccagctg taaccgaaca
     2461 tgcgcgtcgt cttcctctca ccgcgatacc cgcccgagat gcgcgagttc acgcgcggcc
     2521 tcgccgaagt cggcgcgacc gtcctcggag ttggcgacgg acacccggat gacgagctga
     2581 agcggtacct gtccgactac ctcgaggtgc catcactctt cgacgaggct gacgtcatcc
     2641 ggcgcgtcca cgcgtggctc cgcgggcgga gcgtcgatcg catccttgcg aattgggagc
     2701 cgctcgtgat cacggccgcg cgcctgcgcg agatgttcgg catgcccggc atgtcggtcg
     2761 acgccgtccg cggcttccgc gacaagcagc tcatgaagga gcgcgtcgca gcggccggaa
     2821 tccgcgtgcc tcgctcggct cgcgctcaca cgctcgcgga agtgcgcgtc gccctcgaag
     2881 ccacgggtta tcctgcgatc gtcaaaccga tcagcggcgc aggcagcgca gatacctacc
     2941 gcgtcgactc cgaacgcgag ctcgacacga tcgcgccgct gctgcgccac gtcgacgagg
     3001 tgagcgtaga agagttcatc accggcgaag agctcacgta cgacaccgtc tgcatcggcg
     3061 gcaagcccgt gtacgagagc gtcacgcagt acatccccaa cgcgctcgag atgcgctcgc
     3121 aagaatgggt ctcgcccatc atgctctcgg tgcgcgatct ttcgcagccg aacattcaga
     3181 ccggcatcgc gctcggtcgc cgcgtcctcg acgcactcgg catgggcgac ggcatgacgc
     3241 acatggagtg gttccgcaaa cccgacggcg acgtcgtctt cggcgaaatc gcgtgtcgcc
     3301 ccggtggcgc atgcgttgtc gatctcatga actacacgag cgacatcgat ctgttccgcg
     3361 agtgggcacg cgccgtctgc tggcacgcgt tcgaagcgcc caccgaccgc aagtacaacg
     3421 tcggcatcgt gttcaagcgc gcgcacgggc aaggccgcat caccaccatc gccggcctcg
     3481 acgactacta ccggcgcttc ggccgacaca tcgtcgccga tacgctcctt cgcccaggca
     3541 cgccgcgccg cgattggaag cagactctcc tctccgacgg ctacctcgtc gttcgccacc
     3601 ccgactggga caccgcccgc gagatgacct tcgcagccgc caccggcgtc cagctctacg
     3661 cgtcctgagc caccaaagca tgcgccacat cgtcttcatc gctcctcgct tcttcgacaa
     3721 cacgaaccgc ttcctccaag ggttcgtcga gctcgacaac atcacgctca gcgtcatcag
     3781 cctcgatccg gaatccgcga tccccgaatc gatgcacccg aagatcgccg gccactacgg
     3841 cgtcaagagc ctcgacacgt cggagctcac gacggccgcg cgctggctcg ctacttcgat
     3901 cggcccgatc gatcgcctcg tcggatttct cgaagagctg caagtcccgc tcgccgaagt
     3961 gcgcaccgcc ctcgacatcg aaggcctccg cacacaagcc gcgcacaact tccgcgacaa
     4021 ggatcagatg aagacggtcc tgcgcgccgc cggtgtcccg gtcgcccgca gcacgctcgc
     4081 gacgacgcgc gccgagctcg aagccttcat cgccgacgtc ggctatcccg tcatcgtcaa
     4141 gccccgcgcc ggtctcggga cgcgcgccac ctaccgcgta ggcgaacgtg ccgagctcga
     4201 cgcgctcccc gctccctctc ccgccgcccc actccaagtc gaagaattcg tcggcgcgcg
     4261 cgagcacacc tgcgaaaccg tcacgatccg cggcaaaccg atctggcgct cggggacgcg
     4321 ctacttcccg tcgccgctcg aggttctcga cacgccgtgg atccaatacg cagtccttct
     4381 cccgcgcgaa gccgacgatc cgacgtggac caacttccac ccgacgaaca cggccgcgct
     4441 cgccgcgctg ttcggccccg acgcgaccac ttcagccgcc ggcaccgcgc tcactcacat
     4501 ggagtggttc atgcgcgacg acggcacgtc gctcgtcaac gaagtcggcg tgcgaccgcc
     4561 cggcgtccac atcatgccga tgatgggcct cacgcacgac accgatatgt tcgccgactg
     4621 ggcacgcctg atggcgctcg aagagttcac gccgaagccg cgcgtctgcg ccgcaggctc
     4681 cgcgttcttc cgcggcccgg ctcctcgcca aacgcgcggt gcacgcatcg tctccgtcac
     4741 cggcatcgag cgcgccgtcg aagaatgtgg cgactcgctc gtcatcctgc gcacgccgaa
     4801 gatcggccag ccgcgcagcg agcactacga gggcgaaggc tgggcgatcg tcaaacacgc
     4861 gacgacggaa ggcgtgaaga atgccctgcg cacgctgatc gaaaacgtcc agatccgata
     4921 cggctgatcg ccccatgcat cttcggcgag gcgaggtata agaccatcac cgacgcgatg
     4981 acgaacgacg accacagcga cggcttgcta accggcatct cgatcctcgt gctcgaggac
     5041 gatcgcgaca ctctggatct gttcgccggc tcgttgacga agctcggcgg cactgtgcgc
     5101 aaggcctcca ccgttgcggc agccctcacc actctcgcga cgtggcggcc gaacgtgatc
     5161 ctctgcgatc tacacatgcc cgaaacggac ggctacgcgt tcctcgccag cctgcgcgcc
     5221 gccgagatcc tcattcccgt gatcgcgatc agtgccagcc atccaaccgt cgagcgagac
     5281 cgcgcgctcg aagcaggctt cgccgagtat ctcgtcaagc caagccggat cgccgagacg
     5341 gcgctcgtca tcaagaagat cgcgaagcct cgcgcgtgat ctaacggctc gagacgatca
     5401 agtacttctc gccggtcgcc atctgcgcac acgcgcgcag cgtctctaga tcgagcaact
     5461 gatcgagcgt gatccgtgcg gtgtaatggc tcgcgaacgt cgtcctgagc tcgctcgcga
     5521 cgcgcttgaa catgcggccc accgcctcgg ggccgagctt gcgcatcgcg ttcatcacga
     5581 ggtaaccacc gacgctccac gtcagcccga acgttcgcga cagcgtcgtt ggcgcccgat
     5641 ccagcgagcc atagatatag agctgcttga gcgtcgacga gccatagtga ctgaacgtgc
     5701 tcatcgagcg gctcgccacg cgctccatcg cgtcgagaat tcgcccacca agcgagccgc
     5761 cgccgatcgc atcgaacgcg atcgtggctc cagtcgcagc cacggcctcc acgagctccg
     5821 ccgcaaacgt cgacgacgag ctgtcgagca catgctcggc cccgagcgct cgcagctgtt
     5881 cgacatgcgc cggcttgcgg acgatgttca ccaacggcac gccatccgcc ttgcagatct
     5941 tgacgagcat ctggccgagg ttcgacgcgg ccgccgtatg cacgatcgct ttgtgtccct
     6001 cggcgcgcat cgtctcgacg aagctgagcg ccgtcatcgg attg
//
LOCUS       DATLBZ010000689         5219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8053403, whole genome shotgun sequence.
ACCESSION   DATLBZ010000689 DATLBZ010000000
VERSION     DATLBZ010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8053403"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VMZ53_33755"
     CDS             <1..435
                     /locus_tag="VMZ53_33755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33533.1"
                     /translation="NVLGMHPEGTRGKGDDPYTFLPAQPGVGKLALVGKPMVIPAFVL
                     GLSNNLAADVRLNFTKEGRRKHPVVTVFGTPLDYSDLMAEKPRPTLYKKTADRFMAEI
                     KKLGEREKQIRADIASGAISLDDPRWINDRGAPRLYAKEGRF"
     gene            473..2875
                     /locus_tag="VMZ53_33760"
     CDS             473..2875
                     /locus_tag="VMZ53_33760"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS33534.1"
                     /translation="MAVGGDDDTLASSEPTSMGASNSTTTGVAQSEVVAGRYKIVRWL
                     GGGGMGRVYEALDTELDEKVALKVLKTGLSDDALERFRREVKLTRRIQHRNVARMFDI
                     GEHKGEKFLTMELVEGDPLTREISETPMGWTRLRSIAQQICEGLAAAHAAGVIHRDLK
                     PDNVLIERGTDRAVLTDFGIARGQEDPGVTQVGAVVGTPRYMSPEQLNGRELDARSDL
                     FSLGVMLFELATGLRPWPGDNAVAIAVSQATTAPRTIDTTQATLPGYFASIVTACLQM
                     DPRDRPASAAEIAAALVDERPVLVARDNATRVAKPSNAPPIVKPAKKPNAVHEPASAT
                     LAEPTSVAVLPLTCAPADEYLADGLLEDLTDTLSTTPGIRVRPPGFVRGAGGGDPREH
                     GQHLQVDHVVVGTVRRVPTGLRITTRLISVADGFQIWAHKTDCSEADVLSVASELAHG
                     IAQALSTRASSSTRPTDPRAVDFYLRARAELRRFWGSHAQTAADLLEQAVEDAPTSAP
                     IRGAYAYACVQAWVMRSEGELLPRAREAIDRALVLGHGEAFLASAQLHFNLGDSEKGA
                     AELARALVRAPMSAQSHELAGKILIEIEGAAQARQHYETARGLDPGRGLIIDNDLARL
                     DALERKWVEADRRVALLSNDPDTSIAQLGHIANARLSAWRGRMDVLSDTATTFLSRVS
                     SNANSIFRVIIQIQSDRRISEPIWNAAVRAPLPPDRPVRQFMMRLQIFCEVAALLDQR
                     DYALEALTRVVDLGLMDVAWMDNCPLLEKLVELPGYPTLRRRVGERASRVLSAFRGAS
                     TA"
     gene            2915..3307
                     /locus_tag="VMZ53_33765"
     CDS             2915..3307
                     /locus_tag="VMZ53_33765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS33535.1"
                     /translation="MGRAVRGRRLLLIDGDSISRTVLRHAFSTRGHTVSVAATAAEAI
                     AIAASNPPEVVVYDWSFRDESGVGLAKQLRAAVPHPIVIVSLSVLDEPDGFREREDVD
                     DYVVKPALAESVELAFEHALAGVTPRRL"
     gene            complement(3288..3551)
                     /locus_tag="VMZ53_33770"
     CDS             complement(3288..3551)
                     /locus_tag="VMZ53_33770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33536.1"
                     /translation="MALEYKFVELSVVTDESIEEAVNAWVQRGWLLEGIRFVTTEASR
                     RPQMAFISFTRERIGEVEAPDRDPSDQTMPRLKGPRLVKDDEE"
     gene            complement(3551..4531)
                     /gene="hemB"
                     /locus_tag="VMZ53_33775"
     CDS             complement(3551..4531)
                     /gene="hemB"
                     /locus_tag="VMZ53_33775"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HUS33537.1"
                     /translation="MNYPDYRPRRMRRTENLRRMIRETRLAPEDFIYPLFVVPGSGVK
                     KPIGSLPGQFNFSIDKAVDAAAEAADLGIPAVILFGVPERKDSVGSEAWKESGIIQKA
                     IRAIKKARPELVVMADACFCEYTDHGHCGVMHDGELDNDASLENLARTVVSYAKAGVD
                     VVAPSGMLDGFVTACRESLDEDGFDQVAILAYSAKYASAYYGPFREAVDSTPQSGDRR
                     GHQMDYSNVAEAIREVSLDVAEGADIVMVKPALAYLDVIRAVSDEVNMPVCAYNVSGE
                     YAMIEAAAEKGWIDRDRIIMETLTGIRRAGADMIISYHAPYACKLLQKQR"
     gene            complement(4535..>5219)
                     /locus_tag="VMZ53_33780"
     CDS             complement(4535..>5219)
                     /locus_tag="VMZ53_33780"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HUS33538.1"
                     /translation="SIAMPVTKPAPAADPDALVRAMSEGSYDVIMVASARAAHELARA
                     VLAATPIRATLPDVPDVWAVGPATKRALDIAKLPARWPPNVKDGAELARMIAAKMSLA
                     GKRVLVPRAEEGRAEAIAILRNAGAEVVDVIAYRTVPVAVDDENVQEGARILRDGSAT
                     LCCVFAPSQVGALAAIVGALAKVPATFAAIGETTAAALRDAGADRVVVARLPTPEGMA
                     QAVRSVYPP"
BASE COUNT          867 a         1792 c         1704 g          856 t
ORIGIN      
        1 aacgtgctcg gcatgcaccc cgagggcacc cgcggcaaag gcgacgatcc atacacgttc
       61 ctgccggcgc agcccggcgt cggcaagctc gcgctcgtcg gcaagccgat ggtcattccc
      121 gcgttcgtgc tcggtctcag caacaacctc gcggcggatg tgcggctgaa cttcacgaaa
      181 gaaggccgtc gcaagcatcc ggtcgtcacc gtcttcggca cgccgctcga ctattcggat
      241 ctgatggccg agaagccgcg accgacgctc tacaagaaga ccgccgaccg cttcatggcg
      301 gagatcaaga agctcggcga gcgcgagaaa cagatccgcg cggacatcgc atccggtgcc
      361 atcagcctcg acgatccgcg ctggatcaac gaccgcgggg caccgcggct gtacgccaaa
      421 gagggtcgct tctagaacgt caggctgctc gagcgactgc tagaattggc gcatggccgt
      481 cggcggagac gacgacacgc tcgcgagctc cgagcccaca tcgatgggcg cgagcaacag
      541 cacgacgacg ggcgtcgcgc agagcgaggt cgtcgccggc cgctacaaga tcgtgcgctg
      601 gctcggcggc ggcggcatgg gccgcgtgta cgaagcgctc gacaccgagc tcgacgagaa
      661 ggtcgcgctc aaggtcctga agacggggct ctccgacgat gcgctcgagc ggtttcgccg
      721 cgaggtcaag ctgacccggc gcatccagca tcgcaacgtc gctcgcatgt tcgatatagg
      781 cgagcacaag ggcgagaagt ttctgacgat ggagctcgtc gagggcgatc cgctcacccg
      841 cgagatcagc gagacgccca tggggtggac gcggctgcgt tcgatcgcgc agcagatctg
      901 cgagggcttg gcggcagcgc atgcggccgg cgttattcat cgcgatctca agcccgacaa
      961 cgtgctgatc gaacgcggca cggatcgcgc ggtgctcacg gatttcggca tcgcgcgcgg
     1021 tcaggaagat ccgggcgtca cgcaagtcgg tgccgtggtc ggcacgccgc gctacatgtc
     1081 tcccgagcag ctcaacggcc gcgagctcga cgcgcgcagc gatctgttct cgctcggcgt
     1141 gatgctgttc gagctggcga cggggttgcg gccgtggccc ggcgacaacg ccgttgcgat
     1201 cgcggtgtcg caagcgacga cggcgccgcg cacgatcgat acgacgcagg cgacgcttcc
     1261 ggggtacttc gcgtcgatcg tcacggcgtg cttgcagatg gatccgcgcg atcggccggc
     1321 gagcgccgca gagatcgcgg ctgcactcgt cgacgaacgg ccggtgctcg tcgcgagaga
     1381 caatgcgacg cgcgtcgcga agccgagcaa tgcgccgccg attgtgaagc ccgcgaagaa
     1441 accaaacgcc gtgcacgagc ccgcatcggc gacgctcgcg gagccaacct cggtcgcggt
     1501 gctgccgctg acgtgcgcgc cggccgacga gtacctcgcg gacggtctgc tcgaagatct
     1561 gacggacacg ctgtcgacca cgcccggcat tcgcgtgcgg cctcccggct tcgtgcgcgg
     1621 cgcgggcggc ggcgatccgc gcgagcacgg gcagcatctg caggtcgacc acgtcgtcgt
     1681 cggcacggtt cggcgcgttc cgacgggcct gcgcatcacg acgcggctca tctcggtcgc
     1741 cgatggcttt cagatctggg cgcacaagac ggactgcagc gaagcagacg tgctgtccgt
     1801 cgcgagcgag ctggcgcatg gcatcgccca agcgctgtcg acgcgcgcga gcagctcgac
     1861 gaggccgacc gatcctcggg cggtcgattt ctatctgcgt gcacgcgccg agctgcgccg
     1921 cttctggggc agtcatgcgc agaccgccgc cgatctgctc gaacaggccg tcgaggacgc
     1981 gccaacgtcg gcaccgatcc gcggcgccta tgcgtatgcc tgcgtgcaag cgtgggtcat
     2041 gcgctccgaa ggcgagctct tgccgcgcgc acgcgaggcg atcgaccgcg cgctcgtgct
     2101 cggccacggc gaagcgttcc tcgcgtcggc gcagttgcac ttcaatctcg gcgattccga
     2161 gaagggagct gccgagcttg cgcgcgcgct cgttcgcgca ccgatgtcgg cgcagtcgca
     2221 cgagctcgcc ggtaagatct tgatcgagat cgaaggcgcg gcgcaggcac gccagcacta
     2281 cgagactgcg cgcggtctcg atccgggacg cggcctcatc atcgacaacg acctcgcacg
     2341 cctcgacgcg ctcgagcgca agtgggtcga ggccgatcgc cgcgtcgcac tactgtcgaa
     2401 cgatccggac acgtcgatcg cacagctcgg gcacatcgcg aacgcgcgcc tgtcggcgtg
     2461 gcgaggtcgg atggacgtcc tcagcgacac cgcgacgacg tttctgtcgc gcgtcagctc
     2521 gaacgcgaac tcgatcttcc gcgtcatcat ccagattcag agtgaccggc gaatctctga
     2581 accgatctgg aacgccgccg tccgagcgcc actcccgccc gaccgacccg tccgtcaatt
     2641 catgatgcgg ctgcagatct tctgcgaggt cgccgcgctc ctcgaccagc gcgactatgc
     2701 gctcgaggct ttgacgcgtg tcgtcgatct cggtctcatg gatgtcgcgt ggatggacaa
     2761 ctgtccgctc ctagaaaagc tcgtggagct gcctggctac ccaacattgc ggcgccgcgt
     2821 cggagagcga gcctcccggg tcctgtcggc atttcgcggt gcgtcgaccg cttgagccct
     2881 aaacatcgcc acaacgtggc cgattcgttc ggtaatgggt cgggctgtaa ggggtagaag
     2941 gcttctcttg atcgacggcg attcgatttc gcggacggtg ctgcggcacg cgttctcgac
     3001 gcgaggccac accgtgtcgg tcgcggcgac ggccgcggag gcaatcgcga tcgccgcatc
     3061 gaatccaccc gaggtcgtcg tctacgactg gagctttcgc gacgagtccg gcgtgggtct
     3121 cgcgaagcag ctgcgcgccg cggttccgca cccgatcgtc atcgtctcac tctcggtgct
     3181 cgatgagccc gacggatttc gcgagcgtga agacgtcgac gactacgtcg tgaaaccggc
     3241 gctcgccgaa tcggtcgagc tcgcattcga gcacgcgctc gcgggcgtta ctcctcgtcg
     3301 tctttgacga ggcgtggccc cttcagccgc ggcatcgtct gatcgctcgg atcgcgatcc
     3361 ggtgcttcga cctcgccgat gcgttcgcgc gtgaacgaga tgaacgccat ctgcggccgg
     3421 cgcgatgcct cggtggtcac gaagcgaatc ccttcgagca accagccgcg ttgcacccac
     3481 gcgttgaccg cttcctcgat ggactcgtcg gtcacgaccg acagctcgac gaatttatac
     3541 tcgagcgcca ctagcgttgc ttctgcagca gtttgcacgc gtacggcgcg tgatagctga
     3601 tgatcatgtc ggcgccggcg cggcgaatgc ccgtgagcgt ctccatgatg atgcgatctc
     3661 gatcgatcca gcccttctcg gccgccgctt cgatcatcgc gtactcgccg ctgacgttgt
     3721 acgcgcagac cggcatgttg acctcgtcgg agaccgcgcg gatcacgtcg agatacgcga
     3781 gcgcgggctt caccatgacg atgtcagcgc cttcggcgac atcgagcgac acttcgcgga
     3841 tcgcttcggc gacgttggag taatccatct ggtggccgcg gcgatcgccg gactgcggcg
     3901 tcgagtcgac tgcctcgcgg aacggcccgt aatacgccga cgcgtacttc gcggagtacg
     3961 cgaggatcgc gacttgatcg aagccgtcct cgtcgagcga ttcgcggcac gcggtgacga
     4021 aaccatcgag catgccgctc ggcgcgacga catcgacgcc cgccttcgcg tagctgacga
     4081 ccgtgcgcgc cagattctcg agcgatgcat cgttgtcgag ctcgccgtcg tgcatcacgc
     4141 cgcagtggcc gtgatcggtg tactcgcaga aacacgcatc ggccatcacg acgagctcgg
     4201 ggcgcgcctt cttgatcgcg cggatcgcct tctggatgat gccgctctcc ttccacgcct
     4261 ccgacccgac ggagtccttg cgctccggca caccgaacaa gatcaccgcc gggatgccga
     4321 ggtccgcggc ttccgccgcc gcatcgacgg ccttgtcgat cgagaagttg aactgccccg
     4381 gcagagagcc gatcggcttc ttcacgccgc tgcccggcac gacgaacaac ggatagatga
     4441 agtcctcggg ggcaagccgc gtctcgcgga tcatccgccg gaggttctcg gtgcggcgca
     4501 tccggcgcgg ccggtaatcg gggtagttca tcgcttacgg tggatacacc gaccggaccg
     4561 cctgcgccat tccctccggc gtcggcaatc gtgcgacgac aacgcgatcg gccccggcgt
     4621 cacgcagcgc ggcagccgtc gtttccccga tggcagcgaa cgttgccggc accttggcga
     4681 gcgcgccgac gatcgccgcc agcgcgccga cctgcgacgg tgcgaacacg caacacagcg
     4741 tcgcgctgcc atcccgcaag atccgcgcgc cttcctgcac gttctcgtcg tcgacggcga
     4801 ccggcaccgt gcgatacgcg atcacgtcga cgacctccgc gccggcgttc cgcagaatcg
     4861 cgatcgcctc ggcgcgcccc tcttccgcac gcggcacgag cacgcgctta cccgcgagcg
     4921 acatcttcgc ggcgatcatg cgcgcgagct ccgctccatc tttcacattg ggcggccacc
     4981 gcgcaggcaa cttggcaatg tcgagcgctc gcttcgtcgc cggtcccacc gcccacacgt
     5041 cgggcacgtc gggcaacgtc gcccgaatgg gcgtcgccgc gaggaccgcg cgcgcaagct
     5101 catgcgccgc acgtgcgctc gccaccatga tgacgtcgta cgagccctcg ctcatcgcgc
     5161 gcacgagcgc atcaggatct gctgcaggcg cgggcttcgt caccggcatc gcaatgctc
//
LOCUS       DATLBZ010000690         3750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8066558, whole genome shotgun sequence.
ACCESSION   DATLBZ010000690 DATLBZ010000000
VERSION     DATLBZ010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8066558"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2247)
                     /locus_tag="VMZ53_33785"
     CDS             complement(<1..2247)
                     /locus_tag="VMZ53_33785"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS33539.1"
                     /translation="MKVSEGPPRPPGSGRPPSVTATPYKGMGVVKALSQPPPIPAAAR
                     ISKPALRIDTEPEPARAPRSSLVGRSDELVLLREIIDRSIDFQAPQLVTLIGNQGTGK
                     TRLINELVGKLREENRARVFQGAAERDTNGKLIRFGAITSVLRARFELTPNPDEASKL
                     RFAHEVKSVLASDQVGEMLYFLGSFVGIDFPPTPFLRAVADNAKQHGELARTALRRFI
                     EMDSGNGPLVLVLDDLQWADDETIHLVTDLAVGLGGSPVVLLTASRPEMLVRSAGWGE
                     GAVDHTRIDLRNLEQDEAEQMFRNLLSRCKDLPLDTIDSAVEMTGGNPAFLEQLVRLF
                     IENGAIEVTGQTWKLDPDRAAETELPVSIEEAIQARIAALESEERDLLEKAAVFGNVF
                     WVSAVIALTRLERAEPPPANALDIEWGNGEDVRRRLSDLISILADRDYLLTLDAEDSS
                     IPGDVEVVFKHNLERELIAGSIEPSKLGRYYLAAAQWLESKLLALRDEQLEFLAGLYE
                     RGGDKRRAARCYLQGGDRARARYSPEEARNLYEKGLAMLGEADAPARLEALHNLGDVL
                     EQSGKSEEAERYFNQMLQLSWRYDNLNKAGAAYSRLARTFRRRGKYDNAMEHLRRAQE
                     LFERSRDDRGIASTLDDMGRVHWLRGGYSQALDFHRQALTIRRGLGDRRSIALSLANI
                     GRVHHDSGNFKAAIAQFREALDLRRDIGDLVGIVQSLCDLGGVHAEDGNYEQALELLG
                     EARALAHEI"
     gene            2313..2708
                     /locus_tag="VMZ53_33790"
     CDS             2313..2708
                     /locus_tag="VMZ53_33790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33540.1"
                     /translation="MSRALACCMLLLCGCPTVDLGDDPPDVGLCNPRGGLPYFQNEIV
                     PKYLKLSDKTNGCGRSSACHDRAHGLAFDLLNPTSTQNYRLTQNYLNCGSPMQSDLLT
                     KPLAGQVGHGGGDLVQPSSTEETTFLMWF"
     gene            2708..3466
                     /locus_tag="VMZ53_33795"
     CDS             2708..3466
                     /locus_tag="VMZ53_33795"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HUS33541.1"
                     /translation="MRALVLFLVLATAPAAAHADAFLRVIAQKASVRSGPRNGYRELA
                     IVTRNQVFQVLERGTRDFWFKIELEDGTSGWILGDLVFPFEVGEEGQPGAFTRMGRAI
                     KRAILGPSPVPYSHVGLSFSAGIFDQEGVFILRPSWLIDPYWALEGYTGLSPRSDKSL
                     YLNGLTFVLRMAPGAVICPYATLGTGAATVRPKADNFVDKKDTLMALNAGGGLEITFK
                     KQITLRLDARNWTIFDQNVSSNGQEYTGGLAIFF"
     gene            3475..3696
                     /locus_tag="VMZ53_33800"
     CDS             3475..3696
                     /locus_tag="VMZ53_33800"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HUS33542.1"
                     /translation="MRTHLLAALAVLAALATGCGRTAVRASEKEFLADQIMIFDNDPH
                     DTEAEDHVLSNREGASGGHGAGGGGCGCN"
BASE COUNT          622 a         1248 c         1242 g          638 t
ORIGIN      
        1 gatctcgtgg gcaagggcgc gcgcttcgcc gagcagctcg agggcttgct cgtagttgcc
       61 gtcttcggcg tggacgccac ccaggtcgca gagcgattgg acgataccga cgaggtcgcc
      121 gatgtcgcgg cgcagatcga gcgcctcgcg gaactgggcg atcgccgctt tgaagttgcc
      181 gctgtcgtgg tggacgcggc cgatgttagc gagcgagagc gcgatcgagc ggcggtcgcc
      241 gagcccgcgg cgaatcgtga gcgcttggcg gtggaagtcg agcgcctgcg agtagccgcc
      301 gcgcagccag tggacgcggc ccatgtcgtc gagcgtcgac gcgatgccgc ggtcgtcgcg
      361 cgagcgttcg aacagctctt gcgcgcggcg caggtgctcc atcgcgttgt cgtacttgcc
      421 gcgacggcgg aacgtgcgcg cgaggcgcga gtacgcggcg cctgctttgt tcaagttgtc
      481 gtagcgccac gacagctgca gcatctggtt gaagtagcgc tccgcctctt cggacttgcc
      541 ggattgttcg aggacgtcgc cgagattgtg gagagcttcg aggcgcgccg gcgcgtcggc
      601 ttcgccgagc atcgcgaggc ccttctcgta gaggttgcgc gcttcttccg gcgagtagcg
      661 ggcgcgtgcg cgatcgccgc cttgcaggta gcagcgcgcc gcgcggcgtt tgtcgccacc
      721 gcgttcgtag aggccggcga ggaactcgag ctgctcgtcg cgaagggcca acagcttgga
      781 ctcgagccac tgcgcggcgg caaggtagta gcggcccagc ttgctcggtt cgatcgatcc
      841 cgcgatcagc tcgcgctcga gattgtgctt gaacacgacc tcgacgtcgc ctggaatcga
      901 cgagtcctcg gcatcgagcg tcagcagata gtcgcggtcg gcgagaatcg agatgagatc
      961 ggagaggcgg cgccgcacgt cctcgccgtt gccccattcg atgtcgagcg cgttggcggg
     1021 cggcggctcg gcacgctcga ggcgcgtcag cgcgatcacg gccgagaccc agaacacgtt
     1081 gccgaacacg gccgctttct cgagcaggtc gcgctcttcg gactcgagcg ccgcgatgcg
     1141 cgcttggatc gcctcttcga tcgagacggg tagctcggtc tcggcagcgc ggtcgggatc
     1201 gagcttccac gtttgccccg tcacctcgat cgcgccgttc tcgatgaaca agcgcacgag
     1261 ctgctcgagg aacgccgggt taccgcccgt catctcgacg gcggagtcga tcgtatcgag
     1321 cgggaggtct ttgcagcgcg agagcagatt gcggaacatc tgctccgctt catcctgctc
     1381 gaggtttcgc agatcgatgc gcgtgtgatc gactgcgcct tcgccccagc ccgcacttcg
     1441 cacgagcatc tcgggacgcg acgccgtcag cagcacgacc ggcgagccgc ccaagcccac
     1501 cgcgagatcc gtcaccaagt ggatcgtctc gtcgtcggcc cactgcagat cgtcgaggac
     1561 gagcacgagc ggcccattgc cggagtccat ctcgatgaag cggcgcagcg cggtgcgagc
     1621 gagctcgcca tgctgcttgg cattgtcggc gaccgcgcgc agaaacggcg tcggcggaaa
     1681 atcgataccg acaaacgagc cgaggaagta cagcatctcg ccgacctggt cggacgcgag
     1741 cacgctcttg acctcgtgcg cgaagcgcag cttgctcgct tcgtcgggat tgggcgtcag
     1801 ctcgaagcgc gcgcgcagca ccgacgtgat cgcgccgaac cgaatgagct tgccgttcgt
     1861 gtcccgctcg gcggcacctt ggaacacgcg tgcgcggttc tcctcgcgca gcttcccgac
     1921 gagctcgttg atgagccgcg tcttgcccgt gccttggttg ccgatcagcg tgacgagctg
     1981 aggcgcttgg aagtcgatcg agcgatcgat gatctcgcgc aggagcacca gctcgtctga
     2041 acgtccgacc agcgagctgc gcggggcgcg cgccggttcg ggctcggtgt cgatgcgaag
     2101 cgccggcttc gagatccgag ccgcagccgg gatcggtggc ggctggctga gtgccttcac
     2161 gacgcccatg cccttgtacg gagtcgcggt taccgacggc ggtcgaccgg atccgggagg
     2221 acggggcggt ccttctgaca ccttcatcag actatcattc gcgcccggtt acgtgcacct
     2281 acgcgcgcgg acgggttatc gtcagcaaag ggatgagcag ggcactggcc tgttgcatgc
     2341 tgctgctatg cggctgcccg acggtcgatc tcggagacga cccgccggac gtcggcctgt
     2401 gcaatccaag gggcggcctg ccctacttcc agaacgaaat cgttccgaag tacctcaagc
     2461 tctccgacaa gacgaatggc tgcggccgga gctcggcgtg ccatgaccgc gcacacggcc
     2521 tcgcgttcga cctgctgaac ccgacctcga cgcagaacta ccggctgacg cagaactacc
     2581 tcaactgcgg gtcgccgatg cagagcgacc tcctcaccaa gcccctcgcc ggccaggtcg
     2641 gacacggcgg cggcgatctc gttcaaccca gtagcaccga ggaaaccacg ttcctcatgt
     2701 ggttctgatg agagcgctcg tcctcttcct cgtgctcgcg accgcgcccg ccgcggcgca
     2761 cgcggatgcg ttcctccgcg tgatcgcgca gaaggcgtcg gtgcgctcgg gcccgagaaa
     2821 cggctatcgc gagctcgcga tcgtcacgcg caatcaagtg ttccaggtgc tcgagcgcgg
     2881 cacgcgcgac ttctggttca agatcgagct cgaggacggc acgagcggat ggatcctcgg
     2941 cgatctcgtg ttcccattcg aagtcggcga agaaggccag cccggggcct ttacgcgcat
     3001 gggccgcgcg atcaaacgcg ccatcctcgg tccctcgccg gtgccgtact cgcacgtcgg
     3061 cctatcgttc tcggcgggca tcttcgatca ggagggcgtg ttcatcctgc gcccgtcgtg
     3121 gctcatcgat ccgtactggg cactcgaggg ctacaccggc ctgtcgccgc gcagcgacaa
     3181 gagcctctat ctcaatggcc tcacgttcgt gctccgcatg gcgcccggcg cggtcatctg
     3241 tccctacgcg acgctcggca ccggcgcagc caccgtgcgc ccaaaggccg acaacttcgt
     3301 cgacaagaaa gacacgctga tggcgctcaa cgccggcggc ggcctcgaga tcacgttcaa
     3361 gaagcaaatc acgctccgcc tcgacgcacg caactggacc atcttcgatc aaaacgtctc
     3421 gagcaacggc caggagtaca ccggcgggct cgcgatcttc ttctaggaga acccatgcgc
     3481 acacacctcc tcgctgccct cgccgtcctc gccgctctcg cgacgggatg cggacgtacg
     3541 gccgtgcgcg cgtcggaaaa agaattcctc gccgatcaga tcatgatctt cgacaacgac
     3601 ccgcacgata ccgaggccga agatcacgtg ctctcgaacc gcgaaggcgc atcgggcggc
     3661 cacggcgccg gcggcggagg ctgcggttgc aactgaggat cgccatcgcg ctcgttctgc
     3721 tgacgagcag cgcgcacgcg gacgatcgcg
//
LOCUS       DATLBZ010000691         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6549625, whole genome shotgun sequence.
ACCESSION   DATLBZ010000691 DATLBZ010000000
VERSION     DATLBZ010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6549625"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VMZ53_33805"
     CDS             <1..495
                     /locus_tag="VMZ53_33805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33543.1"
                     /translation="NYLRPMMKEQIALSTQDFAKATKEQPTPGSPDTEDSAPIVRQIP
                     EHPVTVWKGCNEKDMEDVLRSIGDAIAIGAPLYNRNTPEGYEACFRVYEGTSLKFQKD
                     GACDGVRKAFDDGILRARTLTSYKQKAWALRDTFDGLINAFGRWCEQDKACMQKFGNK
                     TQHP"
     gene            505..1170
                     /locus_tag="VMZ53_33810"
     CDS             505..1170
                     /locus_tag="VMZ53_33810"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUS33544.1"
                     /translation="MTVLYLFDIDGTLLHAHGSGRGAFDAVMHELHGIADASNGIRYG
                     GKTDHAIVEEIFVARLGRSSTDAERAAFLAAYVPRLQRMLAEEGVEVLRGVTETLEFL
                     AARPAVRLGIATGNIREGAAAKLEAARLASWFSIGGYGCDSRLRPELVARAIERGREG
                     HDVKEVVVVGDTIYDIAAARACGATVCAVATGADPADKLRDADAVFQSMSELPAWHAA
                     RFG"
     gene            complement(1185..1814)
                     /locus_tag="VMZ53_33815"
     CDS             complement(1185..1814)
                     /locus_tag="VMZ53_33815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33545.1"
                     /translation="MRRIALALMFAACSKGASPPPPKPEPTLAPPPVADAAPSPSPVD
                     AAASPPDASMTTPTAPMTAASLLAELIEMSKTGMPTAIQLRDRLAPRATSQKLSGPKL
                     EIPKWARSTSGANDIFMEMTGNVIGVRLEEDSRGWGAYAELFVNDTSLAEIEKVTGKT
                     KFVPRNPDDFHSGETRAAYVERGDKMVRIFVELTKDGKGVQRILVHFET"
     gene            1852..>2637
                     /locus_tag="VMZ53_33820"
     CDS             1852..>2637
                     /locus_tag="VMZ53_33820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HUS33546.1"
                     /translation="MAVIDKVLVANRGEIALRVMRTCRAMGIATVAVYSDADANAPFV
                     KFADEAVRIGAPPARESYLVIPSLLDAARRTGAQAIHPGYGFLSENAQFAEAVTDAGL
                     IFIGPPAEVIRNLGSKQAAKRIAQRAGVPVVPGYEPDDARMEFPVLVKASAGGGGKGM
                     RIVHARSELAEAIERAKSEAKSSFGDDTLLLEKYVERPRHIEIQILGDTHGNVVHLWE
                     RECSIQRRHQKIVEETPSPALDADRRAAMGAAAVALGREVGYVG"
BASE COUNT          437 a          821 c          950 g          429 t
ORIGIN      
        1 aactatctgc gcccgatgat gaaggagcag atcgccctct cgacgcagga cttcgcgaag
       61 gcgacgaagg agcagccgac gcctggctcg ccggacaccg aagactcggc gccgatcgtt
      121 cgccagattc ccgagcatcc cgtcaccgtg tggaagggct gcaacgagaa ggacatggag
      181 gacgtgctgc gctcgatcgg tgacgcgatc gcgatcggcg cgccgctcta caaccgcaac
      241 acgcccgaag gctacgaagc atgcttccgc gtgtacgaag gcacgtcgct gaagttccag
      301 aaggacggcg cgtgcgacgg tgttcgcaag gcgttcgacg acggcatcct gcgtgcgagg
      361 acgctgacct cgtacaagca aaaggcgtgg gcgctgcgcg acacgttcga cggcttgatc
      421 aacgcgtttg gccgctggtg tgagcaggac aaggcgtgca tgcagaagtt cgggaacaag
      481 acgcagcatc cctgagcgcg gtcagtgacc gttctctatc tgttcgatat cgacggcacg
      541 ctgctccacg cgcacggctc gggtcgcggc gcattcgacg cggtgatgca cgagctccac
      601 ggcatcgccg atgcgagcaa cggcattcgc tacggcggca agaccgacca cgcgatcgtc
      661 gaggagatct tcgtcgcgcg cttgggccgc tcgtcgaccg atgcagagcg cgcggcattt
      721 ctcgccgcgt acgtcccgcg cctgcagcgc atgctggccg aagaaggcgt cgaggtgctt
      781 cgcggcgtga cggagacgct ggagtttcta gctgcgcggc cagcggtgag gctcggcatc
      841 gcgacaggca atatccgcga gggcgccgcg gccaagctcg aggcggcgag gctcgcgagc
      901 tggttctcga tcggcggcta cggctgcgat tcgaggctgc gtcccgagct cgtcgcgcgc
      961 gcgatcgaac gcggccgcga aggccacgac gtgaaggaag tcgttgttgt cggcgacacg
     1021 atctacgaca tcgctgcggc gcgcgcctgc ggcgcaacgg tgtgcgcggt cgcgaccgga
     1081 gcggatcccg cggacaagct gcgcgacgcc gacgcggtgt ttcagtcgat gagcgagctg
     1141 ccggcgtggc atgccgcgcg cttcggctga gacccacgcg aagctcaggt ctcgaagtgg
     1201 acgaggatgc gctgcacgcc tttgccatcc ttcgtgagct ccacaaagat gcgcaccatc
     1261 ttgtcgcccc gttcgacata cgccgcgcgc gtctcgccgg agtgaaagtc gtcgggattg
     1321 cgcggcacga acttcgtctt gccggtcacc ttctcgatct cggcgagtga cgtgtcgttg
     1381 acgaacagct cggcgtacgc gccccagccg cgcgagtctt cttcgagccg cacgccgatg
     1441 acgttgcccg tcatctccat gaagatgtcg ttggcacccg atgtcgagcg cgcccatttc
     1501 gggatctcga gcttcgggcc cgacagcttc tgcgaggtcg cgcgcggagc gagccggtcg
     1561 cgcagttgga tcgcggtggg catgccggtc ttcgacatct cgatcagctc ggcgagaagc
     1621 gatgcggcgg tcatcggtgc ggtgggagtc gtcatcgaag cgtccggtgg tgaggccgcg
     1681 gcgtcgacgg gcgacggcga tggtgctgcg tcggcgacgg gcggtggcgc gagcgtgggc
     1741 tcgggcttgg gcggcggtgg tgaagcgccc ttgctacagg cagcgaacat cagggcgagc
     1801 gcgatccttc gcacgcatcc atcgtagccg accgcgtgat agcgtgcccg cgtggctgtg
     1861 atcgataagg tcctcgtcgc gaatcgcggc gagattgcac tgcgcgtcat gcggacgtgc
     1921 cgggcgatgg gcatcgcgac cgtcgcggtg tactcggatg cggatgccaa cgcgccgttc
     1981 gtgaagtttg ccgacgaggc ggtgcgcatc ggtgcgccac ccgcgcgcga gtcgtacctc
     2041 gtgattccgt cgttgctcga tgcggcacgc cggacgggcg cgcaggcgat ccatccgggc
     2101 tacggctttc tgtcggagaa cgcgcagttt gcggaggcgg tgacggatgc gggactcatc
     2161 ttcatcgggc cgcccgcgga ggtgattcgc aacctcggca gcaagcaagc cgcgaagcgc
     2221 atcgcgcagc gtgcgggggt gccggtcgtg ccgggctacg agccggatga tgcgcggatg
     2281 gaattcccgg tgctcgtgaa ggcgtcggcc ggcggcggcg gcaagggcat gcgcatcgtc
     2341 catgcgcgca gcgagctggc agaagcgatc gagcgcgcga agagcgaggc gaagagctcg
     2401 ttcggcgacg acacgctgct cctcgagaag tatgtcgagc ggccgcgcca catcgagatc
     2461 cagatcctcg gcgacacgca tggcaacgtc gtgcatctgt gggagcgcga gtgctcgatc
     2521 cagcgccgcc accagaagat cgtcgaggag acgccctcgc ctgccctcga cgcagatcga
     2581 cgcgcggcga tgggtgcggc ggcagtcgcg ctcggtcgcg aggttggcta cgtgggc
//
LOCUS       DATLBZ010000692         7565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8090814, whole genome shotgun sequence.
ACCESSION   DATLBZ010000692 DATLBZ010000000
VERSION     DATLBZ010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8090814"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..2083
                     /locus_tag="VMZ53_33825"
     CDS             158..2083
                     /locus_tag="VMZ53_33825"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HUS33547.1"
                     /translation="MDALHNLQTIAWVAGGTAAFLLVLLWLGVVTINERESGLVVRRY
                     GRALPPGRIVAVAGEAGYQARMLPPGWHFPLWRWKYKVERVPLIEVAPGEIALVVAKD
                     GAAIPAERVLAREVDCDKFQDAVRFLQEGGEKGRQVGILTAGKYRINPALFDVVTARR
                     APQFGLSPEQLQVFRVPSDRVGIITALDGRPIPTGDLAGPAVKGHDSFQSGQAFIEAG
                     GCRGLQEEVLLSGAWNLNPWFVAVELVSLTEIPIGYVGVVVSYVGGEHLDVTGDGFAH
                     GDLVERGRKGVWVEPLLPGKHPLNTRTMKVELVPTTNIVLNWANRSEAHSYDAGLSSI
                     TVRSRDGFSFGLDVAAIIHVGMKNAPRVISRVGSMQNLVDHVLQPTVGNYFRNSAQEV
                     TVLEFLSARSARQREAFLHIRAAVAAYDVDCIDALIGDITPPAELMKTQTDKKIADEM
                     QRTYDAQREAQMRRQMLERETAAANLQADVVRSEQQVLIQQKVAEARVAAARGEAESL
                     RVQAQAEAEVQRVSAAAQADATRLAAEAEAVALRQRGEATADSERARGQAVAASYEAG
                     IRALGEQSYTAVQLASILGQAGLKLVPDVVLGEGRSNLADVLLAKMATGSAKPAVPVL
                     DVSGLTPANGSNGHVKG"
     gene            2085..2315
                     /locus_tag="VMZ53_33830"
     CDS             2085..2315
                     /locus_tag="VMZ53_33830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33548.1"
                     /translation="MYRDDHEAALCRIEALERENVALTRENARLLQVQPRPRDLNRPK
                     PTSRQAKANDAKEVVVFALGLLVLIVCALATP"
     gene            complement(2321..2725)
                     /locus_tag="VMZ53_33835"
     CDS             complement(2321..2725)
                     /locus_tag="VMZ53_33835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS33549.1"
                     /translation="MINILLVEDDEVDIMTVKRAFAKANITNPLYIAHNGLEALEVLR
                     KEDLPPKRRLILLDVNMPKMNGIEFLRELRKDPALQQISVVVLTTSNEERDRIEAFRL
                     NVAGYLLKPVTFQQFAEVMSTINKYWALMEMP"
     gene            complement(2730..4724)
                     /locus_tag="VMZ53_33840"
     CDS             complement(2730..4724)
                     /locus_tag="VMZ53_33840"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33550.1"
                     /translation="MVVAIDADSESRKVLSLLPYGVLVVDREWRVTFANSEAHRMLGQ
                     TGVTLWELCPELESTAFATAFRYAMSDRAELISESALPSAGWLQARARPTDGGLLITL
                     RQIHAETSETIQAKQAILSGEIGFALTRRDGLREMLQRCADALVRNLDAALARVWTLD
                     AQQAELVLLASSGCAPPDTLDRVPIGRSKIGKIAERGVPYLTNDFQNDPRAGNREWAQ
                     RESIIGFAGYPLRVEGNVIGVLGVYGRKAFGHDITGALATVADSIALGIERKQSDDAR
                     RALETELRGKAEQLELINEIGKNLTSELELAPLVQRVTNLATRLAHASFGAFFYNLQD
                     GSQTFDEVAVAGISREKVPLPIRTNQLVPAAFSGLPVASFFSTQVAVRGGNVIGALVF
                     GHEHAGVFTENTERLITGVAAQAAIAMDNARLFKNARELITELEKTNAELDQFAYVTS
                     HDLKAPLRGIANLSQWIEDDLGERMDEQNRYHMGLLRGRVRRLEGLIDGILHYSRANR
                     LEGSTIEVDVDQLVREIWELLAPPSTAQLVVGELPTLTTMKIPLQQVLMNLMGNAIKY
                     NRDRELTVEVTARRDEADEWVMCVKDNGVGIAPEFHQRIWQLFQTLEARDKVESTGIG
                     LAVVRKIVESRGGRAWIESKPGDGAAFYFSWPQQARTRHG"
     gene            complement(4887..>7565)
                     /gene="uvrA"
                     /locus_tag="VMZ53_33845"
     CDS             complement(4887..>7565)
                     /gene="uvrA"
                     /locus_tag="VMZ53_33845"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUS33551.1"
                     /translation="TIAIQQKSASSNPRSTVGTITEIYDYLRVLYARAGEQRCYQCGG
                     PVGARTAGEIVDELASLPDKTAVTVLAPKSENRKGEFRELFVELRKAGFVRVRIDGMI
                     VRLDDVDALEKKKKHSIELVIDRITINHSDKGRLTDSVETALREGKGKIMVEVPGEKI
                     PRVYSEANACPTCGIGFPELSPQSFSFNSPLGMCVECNGLGDRLAADPDLVVPDATRS
                     IREGAVAVWGEAIAKDSGWTSNIVKSLAKAFKIDLDKPWNKLGEKQKDVLMYGTGDKR
                     VNVQWEGRHSTGEWAMRFEGILAQLERRHRESSSDRTKQHYEQFFRSIPCAVCNGTRL
                     RPESRAVFVNERAIVDVTGMTVKQTYDFITSMKLTGSRAQIAGEVLKEIKNRLSFLLD
                     VGLDYLTLHRSAGTLSGGEAQRIRLASQLGSELSGVLYVLDEPSIGLHQRDNERLIRT
                     LHRLRDLGNTVLVVEHDEATIEAADWVVDFGPGAGRHGGKVIAEGTPDDVKAVTKSIT
                     GRYLAGTERIEVPEARREPSSWVTLSGAREHNLKNVTCKIPLGVMVAVTGVSGAGKSS
                     LINATLHPALNRMLHRSMDRVGPYDALTGLGQIDKAIVIDQQPIGRTPRSNPATYTKA
                     FDLIRELFAQIPQSRTYGYQAGRFSFNVSAKQGGGRCEACEGAGVREVEMHFLPNVFV
                     TCEVCKGRRYNDATLRVKYKDKDISQILDTPVEEAYEMFKHHKQLGRIMQTMIDVGLG
                     YVSLGQPATTLSGGEAQRVKLARELARVQTGRTLYLLDEPTTGLHFGDVKKLLEVLNR
                     LVDSGNTVLVIEHNLDVIKTADWIIDLGPEGGAAGGEIIATGTPEDVAEIARSYTGQF
                     VKPLLDRARRTRASSASGSGGGRSARRERRGLEASP"
BASE COUNT         1224 a         2419 c         2539 g         1383 t
ORIGIN      
        1 agggcacgac caacgacggc gaccgacata cccgaagtct actaccaaaa ctcgcaataa
       61 attacggacc accaagggca cgacctagca gttccaaccg cttacggcgg cacggccgat
      121 gctttttaac gggctcgaac ctcgaaagga ggacgtcatg gatgcgcttc acaatctcca
      181 aacgatcgcg tgggtagcag gcggaaccgc cgcctttctg ctcgtacttc tctggctcgg
      241 cgtcgtcacg atcaacgagc gcgagtccgg cctcgtcgtt cggcgctacg gccgtgcact
      301 gccaccgggc cgcatcgtcg cggtcgccgg tgaagctggc tatcaagcgc gcatgctgcc
      361 gcctggctgg cacttcccgc tgtggcgctg gaagtacaaa gtcgagcgcg ttcctctgat
      421 cgaggttgcg cctggtgaga tcgcgctcgt cgttgcgaag gacggcgcag cgattcccgc
      481 ggagcgcgtg ctcgcgcgtg aagtcgactg cgacaagttc caggacgccg tgcgcttcct
      541 ccaagaaggc ggcgagaagg gccgtcaggt cggcatcctc accgcgggta agtaccgcat
      601 caatccggcg ttgttcgacg tcgtgaccgc gcgtcgcgcg ccgcagttcg ggctctcgcc
      661 cgagcagttg caagtcttcc gcgttccgag cgatcgcgtc ggcatcatca cggcgctcga
      721 cggccggccg attccgacag gtgaccttgc gggtcccgcg gtgaaaggtc acgacagctt
      781 ccagagcggc caggcgttca tcgaggccgg cggctgccgt ggtttgcagg aggaggtgct
      841 cctctccggc gcgtggaacc tcaacccgtg gtttgtcgcg gtcgagctcg tctcgctgac
      901 cgagatcccg atcggctatg tgggcgtcgt cgtcagctac gtgggcggcg agcacctcga
      961 cgtcaccggt gacggcttcg cgcacggcga cctcgtcgag aggggccgca agggcgtgtg
     1021 ggtagagccg ctgcttcccg gcaagcatcc gctcaacacg cgcacgatga aggtcgagct
     1081 cgtgccgacg acgaacatcg tcctcaactg ggccaatcgc agcgaggcgc actcgtacga
     1141 cgccggtttg tcgtcgatca ccgttcgctc gcgtgacggc ttttcgtttg gcctcgacgt
     1201 cgctgcgatc attcacgtcg gcatgaagaa tgccccgcgc gtgatctcgc gagtgggctc
     1261 gatgcagaac ctcgtcgacc acgtgctgca gccgaccgtc ggcaactact tccgcaactc
     1321 ggcgcaggag gtcaccgtgc tcgagttcct ctcggcgcgc agcgcacgtc agcgcgaggc
     1381 gttcctccac atccgtgcag cggtcgcggc ctatgacgtc gactgcatcg atgcgctcat
     1441 cggcgacatc acgccgccgg ccgagctcat gaagacgcag accgacaaga agatcgccga
     1501 cgagatgcag cgcacgtacg acgcgcagcg tgaagcgcag atgcgccgcc agatgctcga
     1561 gcgcgagacc gcggctgcga acctgcaagc ggatgtcgtg cgcagcgagc agcaggtgct
     1621 catccagcag aaggtcgcgg aagctcgcgt cgcggcggcg cggggcgaag cagaatctct
     1681 gcgcgtgcaa gcgcaggccg aagccgaggt gcagcgcgtg agtgcggcgg cgcaagccga
     1741 tgcgacgcga cttgcggctg aagccgaagc ggtggcgctg cgccagcgcg gtgaagcaac
     1801 ggccgactcc gagcgtgcgc gcggtcaagc cgtcgctgcg agctacgaag cgggcatccg
     1861 cgccctcggc gagcagagct acaccgcggt gcagctcgcg tcgatcctcg gccaggccgg
     1921 cctcaagctc gtgcctgacg tcgtgctcgg cgaaggtcgc tcgaacctcg cggacgttct
     1981 gctcgcgaag atggcgaccg gctcggcgaa gccggcagtg ccggtgctcg acgtcagtgg
     2041 cctcacgccg gcaaacggca gcaacggcca cgtgaagggc tgatatgtac cgggacgacc
     2101 acgaggctgc gctctgccgc atcgaggcgc tcgaacgcga gaacgttgcg ctcacgcgcg
     2161 agaatgctcg actgctgcag gtgcagcctc gtccccgcga cctcaatcgg ccgaagccga
     2221 cgtcccgtca agcgaaggcc aacgacgcca aagaggtcgt cgtcttcgcg ctgggactgc
     2281 tcgtgctgat cgtgtgcgcg ttagcgacgc cctgaggcga tcagggcatc tccatcagcg
     2341 cccagtactt gttgatcgtc gacatgacct cggcgaactg ctggaacgtg accggcttta
     2401 ggaggtagcc ggcgacgttg agcctgaacg cttcgatgcg gtcacgctct tcgttcgagg
     2461 tcgtgagcac gacgacgctg atctgctgaa gcgctggatc cttacgcagc tcgcggagga
     2521 actcgatgcc gttcatcttc ggcatgttca cgtccagtaa aatcaggcgg cgcttcggcg
     2581 gcaggtcttc tttgcgcagc acctcgagag cctcgaggcc gttgtgcgcg atgtagagcg
     2641 ggttcgtgat gttcgccttc gcgaatgccc gcttcacggt catgatgtcg acctcgtcgt
     2701 cttcgacgag caggatattg atcacccgtt catccatgtc gcgtcctcgc ctgttgcggc
     2761 cagctgaagt aaaacgcagc gccatcgcct ggcttggact cgatccaggc gcggccgccg
     2821 cgtgattcta caatcttgcg cacgacggcg agcccgattc cggtgctttc gaccttgtcg
     2881 cgcgcttcta gtgtttggaa cagctgccag atgcgttggt ggaactccgg cgcgatgccg
     2941 acgccgttgt ctttcacgca catcacccat tcgtcggctt cgtcgcggcg cgcagtgacc
     3001 tcgacggtga gctcgcggtc gcggttgtac ttgatcgcat tgcccatcag gttcatcagc
     3061 acctgctgga gcggaatctt catcgtcgtg agcgtgggga gctcgccgac gacgagctgc
     3121 gccgtgctcg gtggtgcgag cagctcccag atctcgcgga cgagctggtc gacatcgacc
     3181 tcgatcgtcg agccttcgag acggttcgcg cggctgtagt ggaggatgcc gtcgatcagg
     3241 ccctcgagcc ggcgcacgcg accgcgaagc agacccatgt ggtagcggtt ctgctcgtcc
     3301 atgcgctcgc cgaggtcgtc ttcgatccac tggctgagat tcgcgatgcc gcgcagcggc
     3361 gccttgaggt cgtggctcgt gacgtacgcg aattgatcga gctcggcgtt cgtcttctcg
     3421 agctcggtga tgagctcgcg cgcgttcttg aagaggcgtg cgttgtccat cgcgatcgct
     3481 gcctgcgccg cgacgcccgt gatgaggcgc tcggtgttct cggtgaagac gccggcatgc
     3541 tcgtggccga agacgagcgc gccgatgacg ttgccgccgc gcacggcgac ttgtgtcgag
     3601 aagaagctcg cgaccggcaa ccccgagaac gctgcaggca cgagctgatt cgtgcggatc
     3661 ggcagcggca ccttctcgcg cgagatgccg gcgacagcga cttcgtcgaa cgtttgggag
     3721 ccgtcctgca ggttgtagaa gaacgcgcca aacgatgcgt gcgcgagccg cgtcgcgagg
     3781 ttcgtcacgc gctgcacgag cggcgcgagc tcgagctcgc ttgtgaggtt cttgccgatc
     3841 tcgttgatca gctcgagctg ctcggccttg ccgcgtagct cggtctcgag cgcgcgacgc
     3901 gcgtcgtcgc tctgcttgcg ctcgatgccc agcgcgatcg aatcggcgac ggtggcgagc
     3961 gcgcccgtga tgtcgtggcc aaacgccttg cggccgtaca ctccgagcac gccgatgacg
     4021 ttgccctcga cgcgcagcgg ataaccggcg aagccgatga tcgactcgcg ctgcgcccac
     4081 tcgcgattgc cggcgcgcgg atcgttctgg aagtcgttcg tcaggtaggg gacgccgcgc
     4141 tcggcgatct tgccgatctt cgagcggccg atcggaacgc gatcgagcgt gtctggcggc
     4201 gcgcagccgc tgcttgcgag caggaccagc tcggcttgct gcgcgtcgag tgtccacaca
     4261 cgcgcgaggg ctgcgtcgag gttgcgcacg agagcatccg cgcagcgctg caacatctct
     4321 cgcaggccgt cgcgacgtgt gagcgcgaag ccgatctcgc cggagaggat cgcctgcttg
     4381 gcctggatcg tctccgacgt ttcggcgtga atctgccgca gcgtgatgag cagcccgcca
     4441 tcggtgggac gcgcgcgcgc ttgcagccag ccggcgctcg gaagtgcact ctcgctgatc
     4501 aattccgctc gatccgacat cgcgtagcga aacgcggtcg cgaacgcggt ggattcgagc
     4561 tcggggcaca gctcccacag cgtgacgccc gtctgaccga gcatgcgatg cgcttcagaa
     4621 ttcgcgaagg tcacgcgcca ttcgcggtca acgacgagga ctccatacgg caacaaggac
     4681 aagaccttgc gcgactcgga gtcggcatcg atcgcgacaa ccacgggtcg acgatacggg
     4741 acgcagagcc tgcgtcacgc gcaacgatcg agaggcttcc gtgaggtaat cgactcccga
     4801 tctcacggac gggaagtacg tggcgccgcg tacgtccgta accgagggtt gcggaagttg
     4861 cgtccgtccc ttaacggttg cggaggctag ggcgaggctt cgaggccgcg acgttcccga
     4921 cgtgcggagc gaccgccacc cgagccactc gccgacgagg cgcgggtgcg gcgcgcgcga
     4981 tcgagcagcg gcttcacgaa ttggcccgtg tagctccgcg cgatctccgc gacgtcttcc
     5041 ggtgtgccgg tcgcgatgat ctcgccacct gctgcaccac cctcggggcc gagatcgatg
     5101 atccaatccg cggtcttgat gacgtcgagg ttgtgctcga tgacgaggac ggtgttgccg
     5161 ctgtcgacga ggcggttgag gacctcgagc agcttcttca cgtcgccgaa gtggaggccc
     5221 gtcgtgggct cgtcgagcag gtacagcgtg cggccggtct gcacgcgcgc gagctcacgt
     5281 gcgagcttca cgcgttgcgc ttcgccgccc gacagagttg tcgccggttg gcccagcgag
     5341 acgtagccga ggccgacgtc gatcatggtc tgcatgatcc ggccgagctg cttgtggtgc
     5401 ttgaacatct cgtacgcttc ctcgacgggc gtatcgagga tttgcgagat gtccttgtct
     5461 ttgtatttca cgcgcagcgt ggcgtcgttg tagcggcgcc ctttgcagac ctcgcaggta
     5521 acgaacacgt tcggcaggaa gtgcatctcg acttcgcgca cgccggcgcc ttcacaggct
     5581 tcgcagcgac cgccgccttg cttcgcgctg acgttgaacg agaaccggcc ggcttggtag
     5641 ccgtacgtgc gcgactgcgg gatctgcgcg aacagctcgc ggatcagatc gaacgccttc
     5701 gtgtacgtcg ccgggttgct gcgcggcgtg cggccgatcg gctgctgatc gatcacgatc
     5761 gctttgtcga tctggccgag accggtgagc gcgtcgtacg ggcccacgcg atccatcgag
     5821 cggtgcagca tgcggttgag ggcagggtgc agcgtcgcgt tgatgagcga tgacttgcct
     5881 gcgccggaga cgccggtgac cgcgaccatc acgccgagcg ggatcttgca cgtgacgttc
     5941 ttgaggttgt gctcgcgggc gccgctgagc gtgacccagc tcgacggctc gcgacgagct
     6001 tcggggacct cgatgcgctc ggtgcccgcg aggtagcggc ccgtgatcga tttggtgacg
     6061 gctttgacgt cgtcgggcgt gccttcggcg atgaccttgc cgccgtgacg tccggcgccc
     6121 ggaccgaagt cgacgaccca gtccgccgct tcgatcgtcg cttcgtcgtg ctcgacaacg
     6181 aggacggtgt tgccgaggtc gcgtaggcga tgcagcgtgc ggatcaggcg ctcgttgtcg
     6241 cgttggtgta gaccgatcga cggctcgtcg aggacataga ggacgccgga gagctcgctg
     6301 ccgagctgcg acgcgagccg gatgcgctgc gcttcgccgc ccgagagcgt gcccgccgag
     6361 cgatgcagcg tcaggtaatc gaggccgaca tccagcagga acgagagccg gttcttgatc
     6421 tctttgagga cctcgccggc gatctgcgcg cgcgagccgg tgagcttcat cgacgtgatg
     6481 aagtcgtacg tctgcttcac cgtcatgccg gtgacatcga cgatcgctcg ctcgttgacg
     6541 aacaccgcgc gcgactcggg ccgcagacgc gtgccgttgc agaccgcgca cggaatcgat
     6601 cggaagaact gctcgtagtg ctgcttcgtg cgatcgctcg acgactcgcg gtgacggcgc
     6661 tcgagctgcg cgaggatgcc ttcgaagcgc atcgcccatt cgcccgtgga gtggcggccc
     6721 tcccactgca cgttcacgcg cttgtcgccc gtgccgtaca tcaggacgtc tttctgcttc
     6781 tcgccgagct tgttccacgg cttgtcgaga tcgatcttga acgccttcgc gagcgacttg
     6841 acgatgttcg acgtccagcc gctgtccttc gcgatcgctt cgccccatac ggcgacggcg
     6901 ccttcgcgaa ttgaccgcgt ggcgtcgggg acgacgagat ccgggtctgc agcgagtcga
     6961 tcgccgaggc cgttgcactc gacgcacatg ccgagcggcg agttgaacga gaagctctgc
     7021 ggcgacagct cggggaagcc gatgccgcac gtcgggcagg cgttcgcctc cgagtagacg
     7081 cgcgggatct tttcgcccgg cacctcgacc atgatcttgc ccttgccctc acggagcgcg
     7141 gtctcgacgg agtcggtgag gcggcctttg tcgctgtgat tgatcgtgat gcggtcgatc
     7201 acgagctcga tcgagtgctt cttctttttc tcgagggcgt cgacgtcgtc gaggcgaacg
     7261 atcatgccgt cgatgcggac gcggacgaag cccgccttgc gcagctcgac gaagagctcg
     7321 cggaactcgc ctttgcggtt ctccgacttc ggagcgagca ccgtgacggc ggttttgtcg
     7381 ggcagcgagg caagctcgtc gacgatctcg cccgcggtgc gtgcgccgac cggaccgcca
     7441 cactgatagc agcgctgctc gccggcacgt gcgtagagca cgcgcaggta atcgtagatc
     7501 tcggtgatcg tgccgaccgt cgaacgcgga ttgctcgacg cgctcttctg ctggatcgcg
     7561 atcgt
//
LOCUS       DATLBZ010000693        13680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8090889, whole genome shotgun sequence.
ACCESSION   DATLBZ010000693 DATLBZ010000000
VERSION     DATLBZ010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13680
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8090889"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VMZ53_33850"
     CDS             <1..377
                     /locus_tag="VMZ53_33850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33552.1"
                     /translation="VLAFVVGCGKKDDKGGGAVNKDMTVDQACDKVVVMMGDMGKAIT
                     SAGDDCNKMGDNMDKWANDNKAFMDWAKEQDKDPKKKEEFDTKCKPKLEKAMEPIMGA
                     MAGAMKCADNPKVKAAMEKMGG"
     gene            complement(401..1093)
                     /locus_tag="VMZ53_33855"
     CDS             complement(401..1093)
                     /locus_tag="VMZ53_33855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUS33553.1"
                     /translation="MNRLLGKFALVTGGSSGIGAACATALAAEGAAVLACGRRFSVDE
                     SAAAPALGQIANAHLDVTNEAEVKARFAQLPDLDIVVLSHGTGTFAPIVNASAADLRE
                     MLDVHITGSFFCAREALRRMQAKRKGHIVFIGSHVAHNTFTDCGGYTAAKAGQLGLAR
                     VLGAEAKPYDVRVTSLLAGATDTPIWDDRPGFDRSKMMKPADVAGFVVSIVTRPGIAI
                     DEVTVMPPAGAL"
     gene            1115..1597
                     /locus_tag="VMZ53_33860"
     CDS             1115..1597
                     /locus_tag="VMZ53_33860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33554.1"
                     /translation="MRLVGLVVAMMVVALPAWADTKPPPTTKAPTASDKAFKPYVGKL
                     VLSPDTPPGTLDELPNYLEGNLSKDNEYHRTKGPPWPFHLVGVLAKDAKKVTLVVHDK
                     ADKKLTPLLSIELTPMRRIVIAHAEATIAAGFAANKTYVVRLMTGKTVLAKSELTLHD
                     "
     gene            complement(1680..3065)
                     /gene="trmFO"
                     /locus_tag="VMZ53_33865"
     CDS             complement(1680..3065)
                     /gene="trmFO"
                     /locus_tag="VMZ53_33865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HUS33555.1"
                     /translation="MNSDVDVTVVGGGMAGSEAAWQLAEAGLRVALVEMKPCAMSPAH
                     QSPLLGELVCSNSLRSDDPVAPAGLLKQELRRAGSLVIACADQHRVPAGQALAVERFG
                     FARAITQRLALHPRIRIERRRIEDLPDHPVIVATGPLTEGALGEVIKQELGGDRMYFY
                     DAIAPIVAAESIDWDRAFRGSRWGRDADAPGRDTDGDRAAGGDTGVGDYVNCPMNKAE
                     YDDFVAAVNAGRKVLPHDFEEPRYFESCMPIEVMAERGPQTLRFGPMRPIGLRDPRTG
                     FRPWAVVQLRPENRYQTAYNLVGFQTRLAYPEQQRIFQMIPGLGRAEFLRFGSIHRNT
                     YIDAPSRLGANLELRDRPNVRFAGLLTGVEGYIESCAMGMMVAWLLAAELTGRAAAPP
                     PPTTMMGGLYNHVTQPREANYKYGPTNVNYGLLPPLPDKRKDNRKQRMAERATIDFDA
                     WLAGIAVRAVA"
     gene            complement(3111..3548)
                     /locus_tag="VMZ53_33870"
     CDS             complement(3111..3548)
                     /locus_tag="VMZ53_33870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33556.1"
                     /translation="MAKAAKKKATKSAKKATGGAKGKKKSAAAKSSAKKSAKKPAKKP
                     ATKAKSAAKKSGSAPKAKSAAPSTKAQRATPPRKQAKPQTATPITHDDPNDRADQAVA
                     NAGDNGDNGDNGEGAMDNGAVAEAVDASWDASEGDASDQAQPA"
     gene            3615..4148
                     /locus_tag="VMZ53_33875"
     CDS             3615..4148
                     /locus_tag="VMZ53_33875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33557.1"
                     /translation="MFRLAFACLLAAGCGRIGFQAPPDGPVDGQCPSDPGCVVGGTCD
                     PLPAGTTCGDHLFCDGAGACSIAEREVGCNGPFTGDQLCQQAGFAGALQATGYYWFQC
                     TGYPERVCPGTGWDFANIRCTTWCGQADCAQVPYCGAGQTAYELSGDGTTVFDQLMYG
                     LDCQSFNPGWLVLLRCY"
     gene            complement(4145..6760)
                     /gene="topA"
                     /locus_tag="VMZ53_33880"
     CDS             complement(4145..6760)
                     /gene="topA"
                     /locus_tag="VMZ53_33880"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HUS33558.1"
                     /translation="MSKKKAPPPKKAAAAKGKAKAPAAAAKAKKPDAKKADAKADAKT
                     AAKADPKAKKPAATVEPPAAEAKKPIRGSKEDKKQKKAADEAAAKEIEAESKRKPKKV
                     KPGSALVVVESPAKAKTINKYLGANYIVKASVGHVRDLPKSKIGVDLEKLTFDPIYEV
                     IDGKKKVVAEIRKAAREVETVYLASDPDREGEAIAWHISEEIKDVNTNLRRVLINEIT
                     KKGVTAAIAKPLDIDMNKTDAQQARRILDRLVGYKISPILWNKVQRGLSAGRVQSVAV
                     RLVCEREEEIKAFVPEEYWSVDVLCRHTRTDVEPPEFGARIWKWKGEKAEPKTKDEAD
                     AIANELRAGDAVVASVEKKERRKKPQAPFITSRLQQDAARKLRFSAKRTMALAQRLYE
                     GVELGEEGPTGLITYMRTDSTRISDDALTAVRQHITDTYGKEFLPDEVNTYGNKSNAQ
                     DAHEAIRPTLMEWPPEKVAEALKEHPEGNELTKLYTLIWQRFVASQMVPAVYDQTTID
                     MDRGEAVLRANGQVMKFAGYTKVYEVAETDDAKAEAAEQADKLLPPVEVGDAIKLVEI
                     TPEQHFTQPPPRFSEASLVKELEERGIGRPSTYAAIMSTIVDRGYVEKKEARFWPTEL
                     GILVNGLLVVSFPEIVDSGFTAQMEANLDHVEEGSEDWRKLLKDFYVPFAVELEKAET
                     EMRDVKREEIATEWVCEKCGKPMVIKWGRNGSFLACQGYPECRNTQEVVKNLDGTWEK
                     VPPQTTDEVCETCGAPMTIKRGRFGSFLACTRYPECKTTKPISLGVKCPRPGCGGFIA
                     EKRSRRGKPFYGCSNWAKKGCDFVAWDKPIPQPCPVCQAKFVLKKETKRGITLRCMEC
                     DWRSEGADESEENAA"
     gene            complement(6757..7572)
                     /locus_tag="VMZ53_33885"
     CDS             complement(6757..7572)
                     /locus_tag="VMZ53_33885"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HUS33559.1"
                     /translation="MTSESQVLRPGDAGYPRRLLALGCTQELHVRGNLAVVTDAAPAI
                     AIVGARAASGPSMQRAHAIARHLAERGAHVVSGGALGIDGAAHRGVLAGEGAGQTIVV
                     LGSGVDVAYPSRHAQLFEDVVARGGLLVSMFALGMQPRRGTFLQRNELIAALADTVIV
                     IEAQVKSGSLSTAAHARNLGRPIGATPESAGCKRLIAAGAALVENAGDVMRIIEGNPR
                     VPAPVVLDEDAQRVRDALAQGATGIDAIVRVTGLPVRAVLRALPQLESSARMQ"
     gene            complement(7680..8948)
                     /locus_tag="VMZ53_33890"
     CDS             complement(7680..8948)
                     /locus_tag="VMZ53_33890"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HUS33560.1"
                     /translation="MRKILFALIAVPSVASAQVPGTYPQQAEVPMNLDPGQSNAPKVT
                     PPPKQQPPAQPNVIVVGPNGQVTQGAPGQATPGGPAAAPYYYGGGDPNAGMDYQSDNN
                     DPLFAGPTPELHVVRTGDTLWDICTLYFADPFQWPKVWSYNPQVTNPHWIYPGDLIRL
                     LPRGLFAEQQPTNTPPETGPDTKPADNLPPPQQRSAVGIKQTAFVEKSDIDKSIQVDG
                     SVDEKELLGPGDSIYVSYPSNNVPKVGQRYSVYRPDNSVKNGGKDVGSYVRILGTVEI
                     VSVKQDKKARAVIIDANQEIERGAKIGPLVKQYKNVPPVQPNVDAQGAIVAMLTKDQL
                     IGTGEIIFVDLGKGSGVEVGNRMFVVRRGDALPPKSKTTVGQDDRRFPARAIGEVVIV
                     EVGDKVSVALVTLAVQEMGVGDIVMMQKSQ"
     gene            complement(8945..9784)
                     /gene="ybgF"
                     /locus_tag="VMZ53_33895"
     CDS             complement(8945..9784)
                     /gene="ybgF"
                     /locus_tag="VMZ53_33895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HUS33561.1"
                     /translation="MTITRYVIWVVLASVGCAAPMSALREDNRRLNQSVAEMRAERRA
                     QDRKIRDLENQVALLKEDKRATSPSRDAMPSLPVEVVAPSSVGEGQRIVAYADDGDEI
                     VYEGDAALGRQAHVPTTIDSPRSRDDDDAPARTASRPLSAARTSRVVTRSEPINDAAN
                     NDYRAAVELVKAGKTEDAVAALRTFLKSYPRNDYADNAQYWLGEAFYAQKDYPHALVE
                     FRATIETYPRGNKVPDALLKVGYCYGALGQADKARAVLEQVVNLYPKTEPAALASKRL
                     ETP"
     gene            9902..10741
                     /locus_tag="VMZ53_33900"
     CDS             9902..10741
                     /locus_tag="VMZ53_33900"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS33562.1"
                     /translation="MRVLVVLLCLCGLASADAPALRSAKADYPAEALQEFARGVVWAK
                     LGDDREAMLLFQRANDRSPQANTYWNIGVLYLRQEDLEKALYAFEKALELAPKSDRAE
                     IEKVIKQTKQTKAQWTVWISVDHGPPRALVLVDGVAIGPSPATFEPKPGEHNVQAFSK
                     GGFYAQRDVHYDDQRIDERLSGVMLEPHENKGNVFIDVGFHTTYSTWKQGTTTYSMNK
                     PFPLPVGHYKAVPTEIGRTFCTPIEFDVKSTTDITYVLVDYEDGNSCKRIKRTTVQSL
                     RLP"
     gene            10738..11607
                     /locus_tag="VMZ53_33905"
     CDS             10738..11607
                     /locus_tag="VMZ53_33905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUS33563.1"
                     /translation="MKLAALLVVVGMASNAAAETRKGAPDKYVKAAGDAFAQAMAAED
                     KKDFRKATQLYERAFTIAPHAATAYNLALLKARLESWDEAAALYEIYLTLAPSAPDRK
                     QVEAALAAALSATQKIVVKIEDGFDISDAYVLVDGNIFAKPGFVPKRESDHDDPYFEV
                     PLTGGAHVIDVVSPVTYAHLEVNVDFRLAFSIDLHPPARVDGSLVVSSAGPKLWVQKL
                     GYMAGARLDVSGKQSLPLYDDSTYECVPLVADVKKGNNVTFVYVSPVLKTRTENHPER
                     CRKLTFKQLTLAF"
     gene            11607..13412
                     /locus_tag="VMZ53_33910"
     CDS             11607..13412
                     /locus_tag="VMZ53_33910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33564.1"
                     /translation="MPAFFCPRCGARADRDGPCERDGTERLEVTATSLLGTVVGNYVL
                     VEALGEGGMGAVYRAVHPDIGAEVAIKVLHASPTARAQDFLREAQAVNRVRHESLIKI
                     IDTGYLSDRRPYLVMELLDGISLSDVVGKLSFPLACYVAAEALDALDAVHRESVVHRD
                     LKPPNIFLARDGRVVVLDFGIAKLVERGSQATQSANLVGTPEYMAPEQIRAQPLDRRT
                     DIYAMGVLLYETITGRRPFAAQATFDMLLQHLERPPTSPRDYLPSLPNNIAEIILRAL
                     EKEPSRRYQTAAEMADALRAALASGDQRDDLAAYVDANARAARPVPLPTPSTPKAPAT
                     TPMRGKSDRVAPGDVASTVREVPAPRSSSERALAPSRETAAVSERRTPGHPQQSSTRS
                     RVLWLLGGGALAAAGAVTALVMMRPPAPQQRPPRRGSGDIAALPAPVAVDAALPAAPP
                     DAATFGLLTVTTGPDRAMIFIDGALTGPTPLTIQVSAGQHRVKAVVGTRIAEQDVDVP
                     AGERTNALLSVPAVASPSRSRPLTAAGASTKPNNTASADAAVPPPLTDAAAPTPVTAT
                     PPVETQRASDAGTGTSTEHRDNPNHGQRKGNPYKP"
     gene            complement(13424..13588)
                     /locus_tag="VMZ53_33915"
     CDS             complement(13424..13588)
                     /locus_tag="VMZ53_33915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33565.1"
                     /translation="MLVHVHVLVLVHVLVHVHVHVLVHVLVHVHVLVLVLVCRGTGDA
                     VAADTRQCDG"
BASE COUNT         2255 a         4593 c         4377 g         2455 t
ORIGIN      
        1 tggtgctcgc gttcgtcgtc ggctgcggca agaaggacga caagggcggc ggcgccgtca
       61 acaaggacat gacggtcgat caggcgtgcg acaaggtcgt cgtcatgatg ggcgacatgg
      121 gcaaggccat cacgtctgca ggcgatgact gcaacaagat gggcgacaac atggacaagt
      181 gggcgaacga caacaaggcg ttcatggact gggccaagga gcaggacaag gacccgaaga
      241 agaaggaaga gttcgacacg aagtgcaagc cgaagctcga gaaggcgatg gagccgatca
      301 tgggcgcgat ggctggcgcg atgaagtgcg cggacaaccc gaaggtcaag gccgcgatgg
      361 agaagatggg cggctagccc tctcgctcta gcgatcgttg ctagagcgca ccggccggag
      421 gcatcaccgt cacttcgtcg atggcgatac ccggtcgggt cacgatcgag actacgaagc
      481 ctgccacgtc ggcgggcttc atcatttttg agcgatcgaa tccaggccga tcatcccaga
      541 tcggcgtgtc ggtcgcgccg gcgagcagcg aggtcacgcg cacgtcgtag ggcttcgcct
      601 ccgcgccgag cacacgcgcc aagccgagct gcccggcttt cgccgctgta tagccgccgc
      661 agtcggtgaa cgtgttgtgc gcgacgtgcg agccgatgaa gacgatgtgg cctttgcgct
      721 tcgcctgcat gcgcctcagc gcctcacgcg cgcagaagaa cgagccggtg atgtgaacgt
      781 cgagcatctc gcgcagatcc gcggcgcttg cattcacgat cggcgcgaac gtgccggtgc
      841 catgcgagag cacgacgatg tcgaggtcgg gcagctgcgc gaaccgcgcc ttcacttccg
      901 cttcgttcgt cacatcgagg tgcgcgttgg cgatctgccc gagtgcgggc gcggcagccg
      961 attcatcgac ggagaaccgc cggccacacg cgagcaccgc agctccttcg gcggccaacg
     1021 ccgttgcaca cgcggcaccg ataccgctcg agcctccggt gacgagcgca aacttgccga
     1081 gcagtcggtt cacggccggc agtatactca cgggatgcgg ctcgtcgggc tcgtcgtcgc
     1141 gatgatggtg gtggcgttgc ccgcgtgggc cgacacgaag ccgccgccga cgacgaaggc
     1201 gccgacggca agcgacaagg cgttcaagcc ctacgtcggc aagctcgtgc tctcgccaga
     1261 cacgccgccg ggtacgctcg acgagctgcc gaattatctc gagggcaacc tctccaagga
     1321 caacgagtac caccgcacga aaggcccgcc gtggccgttt cacctggtcg gtgtgctcgc
     1381 caaagatgcg aagaaggtga cgctcgtcgt ccacgacaaa gcggacaaga agctgacgcc
     1441 cctgctctcg atcgagctga cgccgatgcg gcgcatcgtg atcgcccacg ccgaagcgac
     1501 gatcgcggcg ggtttcgcgg caaacaagac gtacgtcgtc cgcctgatga ccggcaagac
     1561 cgtgctcgcg aagagcgagc tgacgctgca cgactgactg cgcgctacgc gacgcggaaa
     1621 agcaacgcgc gacgttaggc cacggcgcgc ggaccgcgac cgcaagccga tgcgtaccgc
     1681 tacgcgacgg cgcggacggc gatgcccgcg agccacgcgt cgaagtcgat cgtcgcgcgc
     1741 tcggccatcc gctgcttgcg attgtctttg cgcttgtcgg gcagcggcgg cagaaggccg
     1801 tagttgacgt tcgtcgggcc gtacttgtag ttggcctcgc gcggctgcgt gacgtgattg
     1861 tagaggccac ccatcatggt cgtcggtggc ggtggcgcgg cggcgcggcc cgtcagctcc
     1921 gcggcgagga gccacgcgac catcatgccc atcgcgcatg actcgatgta gccctcgaca
     1981 ccggtgagca gccccgcgaa gcggacgttc gggcgatcgc gcagctcgag gttcgcgccg
     2041 aggcgcgatg gcgcatcgat gtacgtattg cgatggatgg atccgaagcg caggaactcg
     2101 gcgcgcccga gacccggaat catctggaag atccgctgct gctcgggata cgcgagccgc
     2161 gtctggaagc cgacgaggtt gtacgccgtc tggtagcgat tctccggccg cagctgcacg
     2221 acggcccacg gccgaaagcc cgtccgcgga tcgcgcaagc cgatcggtcg catcgggcca
     2281 aagcgcagcg tctgcgggcc gcgctccgcc atcacttcga tcggcatgca gctctcgaag
     2341 tagcgcggct cctcgaagtc gtgcggcagg actttgcgcc cggcgttcac cgccgcgacg
     2401 aagtcgtcgt actcggcttt gttcatcgga cagttcacgt agtcgccaac gcccgtatcg
     2461 ccgccggctg cacggtcgcc gtctgtgtcg cggcccggag catcggcgtc gcggccccat
     2521 cgcgagccac gaaacgcgcg atcccaatcg atcgactccg cggcgacgat cggcgcgatc
     2581 gcgtcataga agtacatgcg gtcgccgccg agctcctgct tgatcacctc gccgagtgcg
     2641 ccctccgtca gcggtcccgt cgcgacgatc accggatgat ccggcaagtc ctcgatgcgc
     2701 cgccgctcga tccgaatgcg cggatgcagt gccaagcgct gcgtgatcgc gcgggcaaag
     2761 ccaaatcgct cgacggcgag cgcctgcccc gccggcacgc gatgctgatc cgcgcacgcg
     2821 atgacgagcg agcccgcgcg ccgaagctct tgcttgagga gccccgccgg cgcgactgga
     2881 tcatcgctgc gcagcgagtt gctgcacacg agctcgccaa ggagcgggct ctgatgtgcc
     2941 ggcgacatcg cgcacggctt catctcgacg agcgcgacgc gcagccctgc ctcggcgagc
     3001 tgccacgccg cttcgctgcc ggccattccc ccgccgacaa ccgttacgtc gacatcgctg
     3061 ttcatcactt cacccaaatt gcagctcggc gatgaacaac cgcacggagc tcaggcaggc
     3121 tgcgcttgat cggaggcgtc gccttcggag gcgtcccacg atgcgtcgac agcttcggcg
     3181 acggcgccgt tgtccatcgc gccttcaccg ttgtcaccgt tgtcgccgtt gtcacccgcg
     3241 ttcgcgacgg cctgatcggc gcgatcgttc ggatcgtcgt gcgtgatcgg cgttgccgtc
     3301 tgcggcttcg cttgcttgcg cggcggcgtt gcgcgctgcg ctttcgtcga cggcgcggcc
     3361 gacttagcct ttggcgcgct acccgacttc ttcgcggcgg acttcgcctt cgtcgcgggc
     3421 ttcttcgccg gcttcttggc cgacttcttc gcgctcgact tcgcggcggc ggacttcttc
     3481 ttgcccttcg ctccgcccgt cgctttcttt gccgacttcg tggctttctt cttcgctgcc
     3541 ttcgccatgc ccaagacgct aaccgaagtt ggccccgacg gggaacgttc gaccgggagt
     3601 ggcataatga gcgggtgttc cgcctggctt tcgcctgcct gttggccgcc ggatgtggcc
     3661 ggatcggctt ccaggcgccc ccggatggcc ccgtggatgg ccaatgcccg tccgatcccg
     3721 ggtgcgtcgt cggtggcacg tgcgatcccc tccccgccgg gacgacctgc ggcgatcacc
     3781 tgttttgcga cggcgcgggc gcgtgctcga tcgccgagcg cgaggtcggc tgcaacgggc
     3841 cgttcaccgg cgaccagctg tgccaacagg cgggctttgc gggcgcgctc caggcgaccg
     3901 gctactactg gtttcagtgc accggctacc cggagcgagt ctgcccggga accggctggg
     3961 acttcgcgaa catccgctgc acgacctggt gcggccaggc cgactgcgcg caggtcccat
     4021 actgcggcgc cgggcagacg gcctacgagc tgagcggtga tgggacgacc gtgttcgacc
     4081 agctgatgta cgggctcgat tgccagtcgt tcaaccctgg ctggctcgtg ctcttgcgct
     4141 gctactaggc ggcgttttct tcggactcgt ccgcgccttc agagcgccaa tcgcactcca
     4201 tacagcgcag cgtgatgccg cgtttcgtct ctttcttgag cacgaacttg gcttggcaga
     4261 ccggacacgg ctgcgggatc ggcttgtccc acgcgacgaa gtcacagccc ttcttcgccc
     4321 agttcgagca gccgtagaac ggcttgccgc gacgcgagcg cttctccgcg atgaaaccgc
     4381 cgcagccggg acgcgggcat ttgacgccca gcgagatcgg cttcgtggtt ttgcactcgg
     4441 gatagcgcgt gcacgcgagg aacgatccga agcgaccgcg tttgatggtc atcggcgcgc
     4501 cgcacgtctc gcagacttcg tcggtcgtct gcggcgggac tttctcccac gtgccgtcga
     4561 ggttcttcac gacctcttgg gtgttgcggc actcggggta gccctggcac gcgaggaagc
     4621 tgccgttgcg gccccacttg atcaccatcg gcttgccgca cttctcgcag acccactcgg
     4681 tcgcgatttc ctcgcgcttg acgtcgcgca tctcggtctc ggctttttcg agctcgaccg
     4741 cgaacggcac gtagaagtct ttgagcagct tgcgccagtc ttcgctgcct tcctcgacgt
     4801 gatcgaggtt cgcttccatc tgcgcggtga agccgctgtc cacgatctcc gggaagctca
     4861 cgacgagcaa accgttgacg aggatgccga gctcggtcgg ccagaagcgc gcttccttct
     4921 tctcgacgta accgcggtcg acgatcgtcg acatgatcgc ggcgtacgtg gacgggcggc
     4981 cgatgccgcg ctcctcgagc tctttgacga gcgacgcttc ggagaaccgc ggcggcggct
     5041 gcgtgaagtg ctgctcgggc gtgatctcga cgagcttgat cgcatcaccg acctcgacgg
     5101 gcggcagcag cttgtctgct tgctcggcgg cctctgcctt ggcgtcgtcg gtctcggcga
     5161 cttcgtacac cttcgtgtag ccggcgaact tcatcacctg gccattggcg cgcagcacgg
     5221 cctctccgcg atccatgtcg atcgtggtct ggtcgtagac cgccggcacc atctgcgatg
     5281 cgacgaagcg ctgccagatg agcgtgtaga gcttcgtgag ctcgttgcct tcgggatgtt
     5341 ccttgagcgc ttcggcgacc ttctcgggcg gccactccat gagcgtcggg cggatcgctt
     5401 cgtgggcgtc ctgcgcgttg ctcttgtttc cgtacgtgtt cacctcgtcg ggcagaaact
     5461 ctttgccgta cgtatcggtg atgtgttggc gcaccgcggt cagcgcgtca tcgctgatgc
     5521 gcgtcgagtc ggtacgcatg tacgtgatga ggccggtcgg gccttcttca ccgagctcga
     5581 caccttcata gaggcgttgc gcgagcgcca tcgtgcgctt cgcggagaac cgcagcttgc
     5641 gcgcggcgtc ctgctggaga cgcgacgtga tgaacggagc ctgcggcttc ttgcggcgct
     5701 cttttttctc gacgctcgcg accacggcat cgccggcgcg cagctcgttc gcgatcgcgt
     5761 cggcctcgtc cttggtcttc ggctcggcct tctcgccctt ccacttccag atgcgcgcgc
     5821 cgaactcggg cggctctacg tctgtacgag tatgccggca cagcacgtcg accgaccaat
     5881 actcctcggg gacgaacgct ttgatctctt cttcacgctc gcagaccagg cgtactgcga
     5941 cggattgcac gcgaccggcg gagaggccgc gctgcacctt gttccagagg atcgggctga
     6001 tcttgtagcc gacgagacga tcgaggatgc ggcgagcttg ctgcgcatcc gtcttgttca
     6061 tgtcgatgtc gagcggcttc gcgatcgctg ccgtgacgcc cttcttcgtg atctcgttga
     6121 tgagcacgcg gcgcaggttc gtattgacgt ctttgatctc ttcagagatg tgccacgcga
     6181 tcgcttcgcc ttcgcgatcc ggatcgcttg cgagatacac ggtctcgacc tcgcgtgcgg
     6241 ccttgcggat ctccgcgacg accttcttct tgccgtcgat gacttcgtag atcggatcga
     6301 acgtgagctt ttcgaggtcg acgccgatct tcgacttcgg caggtcgcgc acgtggccga
     6361 cggacgcctt cacgatgtag ttggcgccca ggtacttgtt gatcgtcttc gcctttgcgg
     6421 gcgactcgac gacgacgagc gcggagcccg gcttcacctt cttcggcttg cgcttggact
     6481 cggcctcgat ctctttggcg gccgcctcgt ccgccgcctt cttctgcttc ttgtcttcct
     6541 tgctgccgcg aatcggcttc ttggcctcgg cggcaggcgg ctcgacggtc gcagctggct
     6601 tctttgcctt ggggtccgct ttcgccgcgg tcttcgcgtc ggcttttgcg tccgccttct
     6661 ttgcgtctgg cttcttggcc ttggcagcgg cagcgggagc cttcgccttg ccctttgcgg
     6721 cggcggcttt cttcggcggc ggagctttct ttttactcac tgcatcctcg ctgacgactc
     6781 gagttggggc agagcacgga gcacggcacg caccggcagt ccggtcacgc gcacgatcgc
     6841 atcgatacct gtagcgcctt gcgcgagcgc atcacgcact cgctgcgcat cttcatcaag
     6901 gaccacgggc gcgggaacgc gcgggttgcc ctcgatgatt cgcatcacgt cgccggcatt
     6961 ctcgacgagc gctgcgcccg cagcgatgag gcgcttgcag cctgcgctct cgggcgttgc
     7021 accgatcgga cggccgagat tgcgagcgtg cgcggcggtc gagagcgagc cgctcttcac
     7081 ctgcgcttcg atcacgatga ccgtatccgc gagcgccgcg atcaattcat tgcgctgcag
     7141 gaacgtgccg cgacgcggct gcatgccgag cgcgaacatg ctcacgagca atccgccacg
     7201 ggcaacgacg tcctcgaaca gctgggcatg tcgggatgga tacgcgacat cgacgccgct
     7261 gccgagcacg acgatcgtct ggcctgcgcc ttcaccagca agcacgccac gatgcgccgc
     7321 tccatcgata ccgagcgcgc cgcccgaaac cacgtgcgct ccccgttcgg cgaggtggcg
     7381 cgcgatcgcg tgtgcacgct gcatcgaagg accgctcgcg gctcgcgcac ctacgatcgc
     7441 gatggccgga gctgcatccg tgaccaccgc gaggttgccg cggacatgga gctcctgcgt
     7501 gcatccgagc gcgagaaggc gccgcggata tcccgcatct cccggcctga gcacctgcga
     7561 ttcgctggtc acaaggggaa tgcaattagc cacacgcgcc ccggccgtca agtgcgagcg
     7621 agtcggctcg tgcgatttca gccggttgca cgatcgctct acatctatat accggagcgc
     7681 tactgcgact tttgcatcat gacgatgtcg cccacgccca tctcctggac tgcgagcgtc
     7741 accagcgcga ccgacacctt gtcgcccact tcgacaatca cgacttcacc gatcgcgcgc
     7801 gccggaaagc gccggtcatc ctggccgacg gtcgtcttcg acttcggcgg gagagcgtca
     7861 ccgcgacgga cgacgaacat gcgatttccg acctcgacgc ccgaaccttt tccgaggtcc
     7921 acgaagatga tctcgcccgt accgatgagc tgatccttcg tgagcatcgc gacgatggcg
     7981 ccttgcgcgt cgacgttcgg ctgcaccggc ggcacgttct tgtactgctt cacgagcggg
     8041 ccgatcttcg cgccgcgctc gatctcctga ttcgcgtcga tgatcacggc gcgcgctttc
     8101 ttgtcctgct tcacgctgac gatctcgacg gtgccgagga tcctcacgta cgagccgacg
     8161 tccttgccgc cattcttcac cgagttgtcg gggcgataga ccgagtaccg ctggccgacc
     8221 ttcgggacgt tgttcgacgg atacgagacg tagatcgagt cgcccgggcc aagcagctct
     8281 ttctcgtcga cggagccatc gacttggatc gacttgtcga tgtcactctt ctcgacgaag
     8341 gccgtctgct tgatcccgac ggcggaccgc tgctgcggcg gcggaaggtt atccgcgggc
     8401 ttcgtgtcgg gtcccgtctc gggtggtgtg ttcgtcggtt gctgctcggc gaacaggccg
     8461 cgcggaagca gccggatgag gtcgcccgga tagatccagt gcgggttcgt gacctgcggg
     8521 ttgtacgacc agaccttcgg ccactggaac ggatcggcga agtacagcgt gcagatgtcc
     8581 cacagcgtgt cgccggtgcg aacgacgtgc agctccggcg tcgggcctgc gaacagcgga
     8641 tcgttattgt ccgactgata gtccatgccc gcgttcggat cgccgccgcc gtaatagtac
     8701 ggagctgctg ccggtcctcc cggcgtcgct tgtccgggcg cgccttgcgt cacttgtcca
     8761 ttcggaccga cgacgatgac gttcggctgc gcaggcggct gctgcttcgg cggtggcgtc
     8821 accttcggtg cgttcgactg ccccgggtcg aggttcatcg ggacttccgc ttgctgcggg
     8881 taggtgcccg gcacttgtgc cgacgcgacg gacggcacgg caatcagcgc gaagaggatc
     8941 ttcctcacgg ggtctccagt cgcttcgatg ccagcgcagc cggctcggtc ttgggataca
     9001 ggttcacgac ttgctccagg acggcacgcg ctttatcggc ttggccgagc gctccgtagc
     9061 agtaaccgac cttgagaagt gcatccggca ccttgttccc gcgcgggtac gtctcgatcg
     9121 tagcgcggaa ctcgaccagt gcatgcggat agtctttctg cgcgtagaac gcttcgccga
     9181 gccaatactg cgcgttgtct gcgtagtcgt tgcgcgggta gctcttgagg aacgtgcgca
     9241 gtgcggcgac ggcatcctcg gtcttgccag ccttcacgag ctcgacggca gcgcgataat
     9301 cgttgttcgc cgcgtcgttg atcggctcgc tgcgcgtcac cacgcgcgac gtccgcgctg
     9361 ccgagagtgg acgcgacgcg gtgcgcgcag gtgcatcatc atcatcacgc gagcgcggcg
     9421 aatcgatcgt cgtcggaacg tgcgcttgcc gcccgagcgc ggcgtcgcct tcgtacacga
     9481 tctcgtcgcc gtcgtcggcg tacgcaacga tgcgctgacc ttcaccgacg gacgacggcg
     9541 ccaccacttc gacgggaagc gagggcatcg catctcgaga cggcgaggtc gcgcgcttgt
     9601 cttctttgag cagcgcgact tgattctcga gatctcggat cttgcgatcc tgcgcacgac
     9661 gttccgcgcg catctcggcg accgactgat tgagccggcg gttgtcttcg cgaagtgcgg
     9721 acatcggtgc tgcacaaccg acgctcgcga gcacgaccca gattacgtag cgcgtgatgg
     9781 tcacaacgcc gaaatcgtag gccgcctcga cgaagcgagc aaaatttcgc gcatctagtc
     9841 atgaagcgct tcgataagcg agagcccgtc gaggcggggg cgcggtgcta tgcttggcgt
     9901 cgtgcgggtg ctcgtggtgc ttctatgcct gtgcggtctg gcgtcggcgg atgcgccggc
     9961 attgcgctcg gcgaaagcag actatcccgc ggaggcgctg caggaattcg cgcgcggcgt
    10021 cgtttgggcc aagctcggcg acgaccgcga agcgatgttg ctcttccagc gtgcgaacga
    10081 ccgctcgccc caggcgaata cgtactggaa catcggcgtt ctctatctgc gccaggaaga
    10141 tctggagaag gcgctctacg cattcgagaa ggcgctcgag ctcgcaccga aatccgatcg
    10201 cgccgagatc gaaaaggtga tcaagcagac gaaacagacc aaagcgcagt ggaccgtctg
    10261 gatctcggtt gaccacggcc ccccgcgtgc gctcgtgctc gtcgatggag tagcgatcgg
    10321 accttcacca gcaacgttcg agcccaaacc cggagagcac aacgttcaag cgttctcgaa
    10381 aggcggcttc tacgcgcagc gcgatgttca ctacgacgat cagcgcatcg atgagcgact
    10441 cagcggcgtc atgctcgagc cgcacgagaa caaggggaat gtgttcatcg acgtcggctt
    10501 ccacacgacg tattcgacgt ggaagcaagg cacgacgacg tactcgatga acaagccatt
    10561 tccgcttccc gtggggcact acaaagcggt gccgaccgag atcggccgaa cattctgtac
    10621 gcccatcgag ttcgatgtga agtcgacgac ggacatcacc tacgtgctcg tcgactacga
    10681 agacggcaat agctgcaaac gcatcaagcg cacgaccgtg cagtcgctga ggttgccgtg
    10741 aagctggccg cgctgctcgt cgtggtcggc atggcgtcga acgccgcggc cgaaacgcgc
    10801 aaaggggcgc ccgataagta cgtcaaggca gccggcgacg cgttcgcgca agcgatggcc
    10861 gccgaggaca agaaagactt ccgcaaggcg acgcagctgt acgaacgtgc gttcacgatc
    10921 gcgcctcacg cggcgaccgc gtacaacctc gcgctcctca aagctcgcct ggagagctgg
    10981 gacgaggcag cggcgctcta cgagatctat ctgacgcttg cccccagcgc gccggatcgc
    11041 aagcaagtcg aggccgcgct cgccgccgct ctcagcgcca cgcagaagat cgtggtgaag
    11101 atcgaagacg gcttcgatat cagcgacgcg tacgtgctcg ttgacggaaa catcttcgcg
    11161 aagccgggtt tcgtccccaa gcgcgagagc gaccacgacg atccgtactt cgaggtgccg
    11221 ctcaccggag gcgcacacgt gatcgacgtg gtctcgccgg tgacctacgc gcatctcgaa
    11281 gtgaacgtcg actttcgctt ggccttcagt atcgatctgc acccgcccgc gcgcgtcgat
    11341 ggatcgctgg tcgtcagctc tgcgggaccc aagctctggg tgcagaagct cgggtacatg
    11401 gcgggcgcgc gcctcgacgt gagcggcaag caatcgcttc cgctctatga cgactccacg
    11461 tacgagtgcg ttcccttggt ggccgatgtg aagaagggga acaacgtcac attcgtgtac
    11521 gtgagccctg tcctcaagac gcgcacggag aatcaccccg agcgctgccg caagctcacg
    11581 ttcaagcagc tgaccctcgc gttctgatgc ccgcgttctt ctgtcctcgg tgcggtgcgc
    11641 gtgcggatcg cgatggtccc tgcgagcgtg acggaaccga gaggctcgaa gtcaccgcga
    11701 cgtcactgct cggcaccgtc gtcggcaact acgtgctcgt cgaagcgctc ggcgaaggcg
    11761 gcatgggcgc ggtctatcgc gcagtgcatc ccgacatcgg cgccgaggtc gcgatcaaag
    11821 tgctccacgc ctcgccgacc gcgcgagctc aagactttct acgcgaagcg caagccgtca
    11881 atcgcgtgcg ccacgagagc ctcatcaaga tcatcgacac gggctacctc tccgaccgcc
    11941 gcccgtatct cgtgatggag ctgctcgacg gcatttcgct ctcggacgtc gtcggcaagc
    12001 tgtccttccc tctcgcgtgc tacgtcgcgg ccgaagcact cgatgccctc gacgccgtcc
    12061 atcgcgagag cgtggtccat cgcgacctca agccgccgaa catctttctc gcgcgcgacg
    12121 gtcgcgtcgt cgtcctcgac ttcggcatcg caaagctcgt cgagcgcggc agccaagcca
    12181 cccagagcgc gaacctcgtc gggacgccgg agtacatggc gccagaacag atccgcgcgc
    12241 agccgctcga tcgccgcacc gatatctacg cgatgggtgt gctgctctac gagacgatca
    12301 cgggtcgccg cccattcgcc gcgcaagcga cgttcgacat gctgctgcag catctcgagc
    12361 gcccgccgac gtcaccgcgc gactacctgc cgtcgctgcc aaacaacatc gccgagatca
    12421 tcctccgcgc gctcgagaag gagccgagcc gtcgctatca aacagccgcc gaaatggccg
    12481 acgcactccg cgccgcgctc gcttcaggcg accagcgcga cgacctcgcc gcgtacgtcg
    12541 atgccaacgc acgcgccgct cgacccgttc cgctgcccac cccatcgacg ccgaaggcgc
    12601 cggccaccac acccatgcgc ggcaagagcg atcgcgtggc cccaggcgac gtcgcgagca
    12661 cggtgcggga agtcccagcg ccgcgctcgt cgagtgagcg cgccctcgca ccatcgcgcg
    12721 aaacagccgc ggtcagcgaa cgccgcacgc ccggccaccc gcagcaatcc tccactcgta
    12781 gccgcgtgct ctggctgctc ggcggcggag ccctcgccgc cgcaggcgcg gtcaccgcgc
    12841 tcgtcatgat gcgacctcca gcgccgcagc aaaggccgcc gcggcgtggc tcgggggaca
    12901 tcgccgcgct gcctgcgccc gttgcggtcg atgccgcact accggccgcg ccgcccgacg
    12961 ccgccacgtt tggattgctc acggtgacga ccggccccga ccgcgcgatg atcttcatcg
    13021 atggcgcgct caccggcccg acgccgctca cgatccaagt cagcgcaggc cagcaccgcg
    13081 tcaaggcggt cgtcggcacg cgcatcgcgg agcaagacgt cgacgtcccc gcgggtgagc
    13141 gcacgaacgc cctgctctcg gtgcccgccg tcgcatcgcc gtcgcgaagc cgcccactga
    13201 ccgccgcagg cgcgtcgacc aaaccgaaca acacagcatc cgccgacgcg gctgttccgc
    13261 ccccgctcac cgacgcggct gcaccgaccc cagtcaccgc caccccgccc gtcgaaacac
    13321 aacgcgccag cgacgcgggc accggcacgt cgacggaaca ccgcgacaat cccaatcacg
    13381 gccaacgcaa gggcaatccg tacaagccct gactcaccgc gcgtcagccg tcacattgcc
    13441 gagtgtcagc tgccacggca tcgccggtcc cgcgacatac aaggacgagc acgaggacgt
    13501 ggacgtggac gagcacgtgg acgagcacgt ggacgtggac gtggacgagc acgtggacga
    13561 gcacgagcac gtggacgtgg acgagcacgt tgcacgtgga cgagcacgtg gacgtggacg
    13621 agcacgtgga cgaggacgag cacgtggacg tggacgtgga cgagcacgtg gacgtggacg
//
LOCUS       DATLBZ010000694         8409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10323366, whole genome shotgun sequence.
ACCESSION   DATLBZ010000694 DATLBZ010000000
VERSION     DATLBZ010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10323366"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1117)
                     /locus_tag="VMZ53_33920"
     CDS             complement(137..1117)
                     /locus_tag="VMZ53_33920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33566.1"
                     /translation="MTVASRAALIVVLLGSIAHAQAPSQPAPAQPTPQQKEADEAFKK
                     GRDLLKQGNFIDACDEFNKSQRLDPANGTLFNIAQCSEKIGRLATAASAYRELIKKDT
                     NPERLELSRKALAGLEPRIPKIVAKIDSPPKNIIITLDSKAGSTQILPNEAVDANFGE
                     YTLIARAKGYTEMISKFRIGEEGSTKTVEVTLLPGASNSDIVRKPGERSTDKRPRNTR
                     KVIGLSLTGVGAGALVAGGVFGIQARGKWNDAKDACGGATCGTQESLDRANLLGDQAR
                     SKATLSTVFIAGGALLAGAGIALWVTAPKSMQVTPTASDTGAGVTLSGSF"
     gene            complement(1101..2780)
                     /locus_tag="VMZ53_33925"
     CDS             complement(1101..2780)
                     /locus_tag="VMZ53_33925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33567.1"
                     /translation="MYGPGSVVAGKYRIEHVLGQGGMGYVVAATHLDLGTPIALKFLH
                     EHMLENRELVDRFKREARASAQLRGENVCRVSDVGTAENGQPFIVMELLQGQDLNSVV
                     KERGALTPQVTAEIMLQTCLALAEAHMVGIVHRDVKPGNLMLTTRPDGTMCIKVLDFG
                     VAKAPEDTNFSLTQTSSVVGSPGYMSPEQLKSSKGATVRSDIWSLGIVMYELVSGVKP
                     WQGESITELALKVAMDPCPPLAGRVPWEFEAVVMHCLEKDPAKRYQDVADLAVALATF
                     AGPRGPEMARAVARVLRGAHTPVPVTPASSLQTHAMPVPSPVPVSTPTTLRHATGAVA
                     QSASQVTARKKKGAWKVPLIMGGGAAVGIAIALVIVSGNKKQATPAKQETETHEMASA
                     PPTPATTPSGDDEQAKKEAAGWAAESAQQEDVAKKEADLAKKEAAKAEAAKLADEKKE
                     ADLAKKEAAKAEAAKLAAEKKEADLAKREAAKAEAAKVAAAKAEAKAEAAKAAAAKAE
                     AKKAAAAKAAAAKVAAAKAAAAKAAAAKTSSTTKTTTTKTTKSKDIGDSRE"
     gene            2832..3959
                     /locus_tag="VMZ53_33930"
     CDS             2832..3959
                     /locus_tag="VMZ53_33930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33568.1"
                     /translation="MWRHVCLGALLAASQPACSKILGIEDFALTPDAPPDAPGPDAQL
                     CWGSGLAPVCITTPAAGVKVLDAALDTDTLGTCTDLIAQPAGPELCVIAANQINISNT
                     VTATGARPLMLLATDTINIGGTLDVSSKRTAAKAGAGANFMGCGAGATPPTTATAGGG
                     GAGGTFGVRGTNGGDGFVNLATVNNNGVSGAILGLTFVRGGCKGQDGGDISATTQGGP
                     GGSSGGAVYLFAATSITVDGNIYASGAGGASGALHTGGAGGGSGGLIGLESPTITVNG
                     IIAANGGNGSAGSSTTAGGAGPDGATMALNTQPAAATNGANGGGTGGRGVTGMTPTAG
                     TTGGAGTVSGGAGGGGGGGNGIVWVKGTLTGTMIAPAPTLN"
     gene            3965..5032
                     /locus_tag="VMZ53_33935"
     CDS             3965..5032
                     /locus_tag="VMZ53_33935"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HUS33569.1"
                     /translation="MLFDTVTFRCGQQLANRIALAPLTNKQSHVDGLLSDAELAFLAR
                     RADGGFGLISTCAAYVALDGKAWDGELGIDRDACIPGLTRLAARLHTTQAKAIVQLFH
                     GGARADAALTGQSVWSASAFQPGVREATADDIERTTAAFVEAAARAKTAGFDGVELHG
                     AHGYVLSQFLSRTMNTRTDAWGGELAHRARFVREVARGVRARCGASFIVGVRLSPEDF
                     GQAKGLDLDESLEVARWLADDGVDFIHASLWDVAKNAVKRPDAHPLALFRAALPTDVV
                     IVTAGKVWTRSDAEHALAHGAGVVALGRSAILNPDWPTTVARGADPTRPPITRAELAA
                     RAVSPPFQQYLTNWKDFVAGD"
     gene            5082..>8409
                     /locus_tag="VMZ53_33940"
     CDS             5082..>8409
                     /locus_tag="VMZ53_33940"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HUS33570.1"
                     /translation="MSSPYRLYSALVFASFLFAAQRSDAHTITVDPVAYQGVYQVDGG
                     AQKTSTATFDLAAGTHTVSVVSSTFTFVVDAAGQVSSSQNPAQALGSTLRFKNVTINV
                     DPAAYDGRYFLRSQFSQQFVGPHSFVVLPSLTYDMSNIVSLWSFSVDALGNVLCSHAA
                     STCSGNTLRFNTTAITVAPGAYTGRYFLRSHFTEQFTGSHTFIVIRGLEEHFSNVTDL
                     LPFNVDATGNVVISSPAATGAGSTLSLNTTTMTVDPGQYTGLYFIRSYFTQNVSGVRT
                     FAVIPGLLHEFSNISGLVGFRVDASGNIVISSPSAVGIGSTLKLNNTTITVDPGAYTG
                     RYYVRSYFTTQFSGQHSYVVVPGMQHELSNITDLASFNVTGTGTITSTHPAITGSGST
                     LKVNTTSITATPSDPNVNYSLQSYPGVTFRGPHDFIVMLGSAHSVVANGQLAQFRVGS
                     PCSVTPSSVIAPPTSFALACTPTPPADSTPPVVRIDATPALAASADLVVTGNVRDNVG
                     VASAAFIVNGVRGPDLGIAPGGDVSATLHLSEGANTIVLQATDAAGNPASTSAVIVVD
                     TTAPAISIESPAADQAVSTQSLSVVVTVGDASPTTVTIDGTVYRLDSSGTIVATVDLG
                     GDGYHDVVVSALDAAGNSSSRAVRVLLDTTAPLVEADVDNGTVFGPLPDHLLPVTFRV
                     DSMTATTVTLLSGDVYELARGGGLIHALVPLSEGVTTFAISAVDEAGRTSTLSGTVVY
                     DTTAPRILDLSPTGGQSVRGVVEIGESIADDTSGVAAVLVTVDGIMVGTVRDGSVWNG
                     SFDTTTLADGTHTIDVFATDVAGNGSVSSRTFTVDNTAPIATIRAPLAGALVRGTISI
                     DVEASDTTSGLGGVDVVVDGTSLGACSQSPCRVAFDTTTLPDGPFAISASATDRAGNR
                     SVVASITAIADNSAPSDFLVSPRSGDIVADSFTLQLAIHDASFASVQCTLDGATIATS
                     TNPAFTTTVTTTGLLDGDSVVRCVATDGAGNVGVSSATITIARWTARISPTTLNLRSK
                     GGATSVTAVIEGRGTQSLTGVTVAVPGGSPVAATPIAVGDDDHDGIPDVVLKIDRQAL
                     IASSRGALAANAIQAGVPFNV"
BASE COUNT         1346 a         2943 c         2632 g         1488 t
ORIGIN      
        1 accaaacgcc cgcgctaaaa ccaaacgcac gcgctcgaac caaaccaaac gcacgcgctc
       61 gaaccaaacg cccgcgctcg aaccaaccaa acgcacgcgc tagagcgcga ggcgcgcgct
      121 agaggcgagg cgcgcgctag aacgagccgc tgagcgtgac gccagcgccg gtgtcgctcg
      181 cggtcggcgt cacctgcatc gacttcggcg cggtgaccca cagcgcgatg cccgcaccgg
      241 cgagcaaggc accgccggcg atgaagaccg tcgacagcgt cgctttgctc cgcgcttggt
      301 cgccgaggag gtttgcgcgg tcgaggctct cttgcgtgcc gcaggttgcg ccgccgcacg
      361 cgtccttcgc gtcattccac ttgccgcgcg cctgtatgcc gaacacaccg cctgccacga
      421 gcgcgcccgc gccgacgcct gtgaggctga ggccgatcac cttgcgcgtg ttgcgcggcc
      481 gcttgtcggt gctgcgctcg cccggcttgc gtacgatgtc gctgttgctc gcaccgggaa
      541 gcagcgtgac ctcgacggtc ttcgtcgagc cctcttcgcc gatgcgaaac ttcgagatca
      601 tctcggtgta gcccttcgcg cgcgcgatga gcgtgtattc gccgaagttc gcgtcgacgg
      661 cttcgttcgg caggatctgc gtcgagccag ccttgctgtc gagcgtgatg atgatgttct
      721 tcggcgggct gtcgatcttc gcgacgattt tcgggatgcg gggctcgagg ccggcgagcg
      781 ctttgcgcga gagctcgagc cgctcggggt tcgtgtcctt cttgatcagc tcgcggtatg
      841 ccgatgccgc ggtggcgagg cggccgatct tctcgctgca ctgcgcgatg ttgaagagcg
      901 tgccgttcgc cggatcgagg cgctggctct tgttgaactc gtcgcatgcg tcgatgaagt
      961 tgccttgctt gaggaggtcg cggcccttct taaaggcttc gtcggcttcc ttctgctgtg
     1021 gcgtcggctg ggcgggcgcc ggctgggagg gcgcctgcgc gtgcgcgatg ctgccgagca
     1081 acacgacgat caacgcggct ctactcgcga ctgtcaccga tgtccttcga cttggtcgtc
     1141 ttcgtggtcg tcgtcttcgt ggtcgaagac gtcttcgccg cagctgcctt cgcggcggcc
     1201 gcctttgcag cagccacctt tgcagcagca gcctttgcag cagcagcctt cttcgcttcc
     1261 gccttcgctg cggcggcctt tgcagcttct gccttcgctt ccgccttcgc cgcagcgacc
     1321 ttcgcggcct ccgcttttgc ggcttccctt ttcgcgaggt ccgcttcctt cttctcggca
     1381 gcgagcttcg cggcctccgc ttttgcggct tccttcttcg cgaggtccgc ttccttcttc
     1441 tcgtcggcga gctttgcagc ctcggccttg gccgcctcct tcttcgcgag gtccgcttcc
     1501 ttcttcgcga cgtcttcctg ctgtgcggac tctgccgccc aaccggccgc ttctttcttc
     1561 gcctgctcgt cgtcgccgct cggcgtcgtc gccggcgtag gcggagcgga tgccatctcg
     1621 tgggtctcgg tctcctgctt cgccggtgtc gcttgcttct tgttgccgct cacgatcacg
     1681 agcgcgatcg cgataccgac cgctgcgccg ccacccatga tgagcggcac cttccacgcg
     1741 cccttcttct ttcgagctgt gacctgcgat gcagactgcg cgaccgcgcc cgttgcgtgg
     1801 cgaagcgtcg tcggtgtcga gaccggaacc ggcgagggca cgggcatcgc gtgcgtctgc
     1861 agcgacgacg ccggcgtgac gggcaccggt gtgtgcgcac cgcgcagcac gcgagcaacg
     1921 gcgcgcgcca tctcgggacc gcgcggcccc gcaaacgtcg cgagagcgac tgcgagatcg
     1981 gcgacgtctt gatagcgctt cgcgggatcc ttctcgaggc agtgcatcac gaccgcctcg
     2041 aactcccacg gcacgcgacc cgcgagcggc gggcatggat ccatcgcgac cttgagcgcg
     2101 agctcggtga tcgattcgcc ttgccacggc ttcacgcccg atacgagctc gtacatcacg
     2161 atgccgagcg accagatgtc gctgcgcacc gtcgcgcctt tgctcgactt gagctgctcg
     2221 ggagacatgt acccgggcga accgacgaca ctcgacgtct gcgtcaagct gaagttcgtg
     2281 tcttccggcg ccttcgcgac gccgaagtcg aggaccttga tacacatcgt cccgtccgga
     2341 cgcgtcgtca gcatcaagtt gcctggcttc acatcgcggt gaacgatgcc gaccatgtgc
     2401 gcctcggcaa gtgcgaggca cgtctgcaac atgatttcgg ccgtgacctg cggcgtgagc
     2461 gcaccgcgct ccttcacgac ggaattgagg tcctgcccct ggagcagctc catcacgatg
     2521 aacggctgcc cgttctcggc cgtgcctaca tcggagacgc gacacacgtt ctcgccgcgt
     2581 aactgcgccg aggctctcgc ttcgcgcttg aagcggtcga cgagctcgcg gttctcgagc
     2641 atgtgctcgt gcaagaactt gagcgcgatc ggcgtgccca gatcgaggtg cgtcgcggcc
     2701 acgacgtagc ccatgccgcc ctgcccgagc acgtgctcga tgcggtactt accagctacg
     2761 acgctgccgg gtccgtacac cgatccgatc ataaaccact gcgacggacc cggcatggta
     2821 acttgttaga tgtgtggcgc catgtgtgcc ttggggccct acttgcggcg agccagccag
     2881 cttgcagcaa gatcctcgga atcgaagact ttgcgctgac cccggacgct ccgccagacg
     2941 cgcctgggcc cgacgcccag ctgtgctggg gatccggtct cgcgcccgtc tgcatcacga
     3001 cgcctgcggc cggcgtcaaa gtcctcgacg ccgccctcga cacagacacc ctcggcacgt
     3061 gcacggatct catcgcgcaa cccgcgggtc ccgagctgtg cgtgatcgca gcgaaccaga
     3121 tcaacatcag caacacggtc accgcgacgg gcgcacgtcc actgatgctg ctcgcgacgg
     3181 acacgatcaa catcggcggc acgctcgacg tctcgagcaa gcgcaccgca gcgaaggcag
     3241 gcgccggcgc gaacttcatg ggctgcggcg caggcgcgac accaccgacg accgcaaccg
     3301 ccggtggcgg cggcgctggc ggaacgttcg gcgtccgcgg cacgaacggc ggcgacggat
     3361 tcgtcaatct cgccaccgta aataacaacg gcgtatccgg cgcgatcctc ggactgacgt
     3421 tcgtgcgcgg cggctgcaaa ggccaagacg gcggcgacat ctccgcgacc acacaaggtg
     3481 ggccgggcgg ctcgagcggc ggcgcggtct acttgttcgc agcaacgtcg atcaccgtcg
     3541 acgggaacat ctacgcatcg ggcgcaggcg gcgcgtccgg cgcactgcac accggcggcg
     3601 caggtggtgg ctccggcggg ctcatcggac tcgagtcgcc gacgatcacc gtaaacggca
     3661 tcatcgcggc gaacggcggc aacggaagcg caggcagctc gacgacggcc ggcggagccg
     3721 gaccagacgg cgcgacgatg gcgctcaaca cgcagcccgc agcggcgacg aatggcgcaa
     3781 acggcggcgg cacgggcggt cgcggcgtca ccggcatgac accgacggcg ggaacgacag
     3841 gcggcgcagg cacggtctca ggcggtgccg gcggcggtgg cggcggcggt aacggaatcg
     3901 tttgggtgaa gggcacgctc accggaacga tgatcgcgcc cgcgccgacg ctaaactgac
     3961 cgcgatgttg ttcgacaccg tcacgttccg ctgcggtcag cagctcgcaa atcgcattgc
     4021 gctcgcaccg ctcacgaaca agcaatcgca cgtcgacggc ctgctctccg acgcagagct
     4081 cgcgttcctc gcgcgccgcg ccgacggcgg cttcggcctg atctcgacgt gcgccgcata
     4141 cgtcgcgctc gacggcaaag cgtgggacgg cgagctcggc atcgatcgcg acgcatgcat
     4201 ccccggcctg acgcgcctcg ccgcccgcct tcacacgacg caggccaaag cgatcgtcca
     4261 gctgtttcac ggcggcgcac gagccgacgc tgcgctcacc gggcaatccg tgtggagcgc
     4321 gtccgcgttc cagcccggcg tgcgcgaagc cactgccgac gacatcgagc gcacgaccgc
     4381 agcgttcgtc gaagccgccg ctcgcgcgaa gactgccggc ttcgacggtg tagagctgca
     4441 cggcgcgcac ggctacgtcc tgtcgcaatt cctctcacgc acgatgaaca cgcgcaccga
     4501 cgcgtggggc ggcgagctcg cacatcgcgc gcgcttcgtt cgcgaggtcg cacgaggagt
     4561 ccgcgcacgg tgcggcgcga gcttcatcgt cggcgttcgc ctctcgcccg aagacttcgg
     4621 tcaggcgaag ggcctcgatc tcgacgagag cctcgaggtc gcgcgttggc tcgcagacga
     4681 cggcgtcgac ttcatccacg catcactctg ggacgtcgcg aagaacgcgg tcaaacgacc
     4741 cgacgcgcat ccgctcgcgc tgttccgcgc cgcgctgccc accgacgtcg tcatcgtcac
     4801 cgcaggcaaa gtctggacgc gctccgatgc cgagcacgcg ctcgcacacg gcgccggcgt
     4861 cgttgcgctc ggccgctccg cgatcctcaa tcccgattgg ccaacgaccg tggcgcgagg
     4921 agccgaccca acgcggccac cgatcacacg cgccgagctt gccgcgcgag cggtctcgcc
     4981 gccgtttcag cagtacctga caaactggaa agacttcgtc gcaggcgatt aaccaccacg
     5041 tacccccacg cgagagtgcc gatggtttac ggttgtgtct tatgtcttcg ccgtaccgtc
     5101 tctacagcgc gttggtattc gcgtcgttct tgttcgccgc acagcgcagc gacgcacaca
     5161 cgatcaccgt cgatcccgtt gcctaccagg gcgtctatca agtcgacgga ggcgcacaaa
     5221 agacgagcac tgctacgttc gatctggccg ccggtacgca cacggtgagc gtggtgtcgt
     5281 cgacgttcac ctttgtcgtc gacgccgcgg ggcaagtctc ctcctcgcag aatccagcac
     5341 aggcgctcgg tagcacgctg cgcttcaaga acgtcacgat caacgttgat ccggctgcgt
     5401 atgacggccg ctatttcttg cggtcgcagt tcagccagca attcgtcgga cctcactcgt
     5461 tcgtcgtgct gccgagcctg acgtacgaca tgagcaatat cgtctcgctg tggtcgttct
     5521 ccgtcgatgc gctcggcaac gtcctctgct cgcatgcggc gtcgacctgc agcgggaaca
     5581 cgctgcggtt caataccacc gcgatcacgg tcgcccccgg agcgtacaca ggtcgctact
     5641 tcctacgctc gcacttcacc gagcagttca cgggttcgca tacgttcatc gtgattcgcg
     5701 gcctcgagga gcacttcagc aacgtcaccg atctgttgcc gttcaacgtc gacgcgaccg
     5761 gcaacgtcgt gatctcgagc cctgccgcca ccggcgccgg ctcgacgctg agcctcaaca
     5821 cgacgacgat gaccgtcgac cctggccagt acacgggcct ctacttcatc cgctcctatt
     5881 tcacgcaaaa cgtgagcggc gtgcggacgt tcgccgtgat cccgggcctc ctgcatgaat
     5941 tcagcaacat ctccggcctc gtcggtttcc gcgtggacgc cagcggcaac atcgtcattt
     6001 ccagcccgag cgccgtcggc atcggctcga cgctcaagct gaacaacacc accattacgg
     6061 tcgacccggg tgcctacacg ggccgctact acgtgcgctc ctacttcacg acgcagttct
     6121 cggggcagca ctcgtatgtg gtcgttccgg gaatgcagca cgagctgtcg aacatcaccg
     6181 acctcgcgtc gttcaacgtg acaggcacgg gcaccattac gtcgacgcat ccggcgatca
     6241 ccggcagcgg ctcgacgctg aaggtcaaca cgacgtcgat tacggcgacg ccaagcgatc
     6301 ccaacgtcaa ctactcgctg cagtcgtatc cgggtgtcac gttccgtggt ccccacgatt
     6361 tcatcgtgat gctcggcagc gctcacagcg tcgtcgcgaa cggccagctt gcacagttcc
     6421 gcgtcgggtc accatgcagc gtgacgccgt cgtcggtgat cgcgccaccg acgagctttg
     6481 cgctcgcatg tacgccgacg ccaccggcgg attcgacgcc gcctgtcgtg cgcattgatg
     6541 cgacgcccgc gctcgctgcc agtgcggacc tcgtcgtcac cggcaacgtt cgtgacaacg
     6601 tcggcgtcgc ctcggcagcg ttcatcgtca acggcgtgcg cggtccggat ctcggcatcg
     6661 ctccgggcgg ggacgtgtct gccacgctcc atctcagcga gggtgccaac acgatcgtgc
     6721 tgcaagcgac agacgccgcc ggaaatcccg catctacgtc cgccgtcatc gtcgtcgaca
     6781 cgactgcgcc tgcgatctcg atcgaaagcc cggctgccga ccaagctgtg agcacacagt
     6841 cgctatcggt cgttgtcacc gtcggcgatg cgagcccgac gaccgtcacg atcgacggaa
     6901 ccgtgtatcg cctcgactcg agtggaacga tcgtcgcgac cgtcgatctc ggcggcgacg
     6961 gttatcacga tgtcgtcgtc agcgcgctcg acgctgcagg gaattcgtcc tcgcgcgcgg
     7021 ttcgcgtgct gctcgacacc actgccccgc tcgtcgaggc cgacgtcgac aacggaaccg
     7081 tgttcgggcc gctgccggat cacctgctgc ctgtcacgtt ccgcgtcgac tcgatgacgg
     7141 cgacgaccgt cacgttgctc tccggcgacg tctacgagct tgctcgtggc ggcggactca
     7201 ttcacgcgct cgtccccctg agcgaaggcg tgacgacgtt cgccatttcc gcggtcgacg
     7261 aagcgggacg cacctcgacg ctgtccggca cggtcgtcta cgacaccacg gcgccgcgca
     7321 tcctcgacct gtcgcccaca gggggccagt ccgtccgcgg cgtcgtcgag atcggcgaat
     7381 caattgcgga cgacacgtcg ggcgtagcgg ccgtcctcgt caccgtcgac ggcatcatgg
     7441 tcggtacggt tcgcgatggc tccgtctgga acggctcgtt cgacacgacg acactcgccg
     7501 acggcacgca caccattgat gtgttcgcca cggacgtggc gggcaacggc tcggtgtcat
     7561 cgagaacgtt cacggtcgac aacacggcac ccatcgcaac gattcgcgcg ccgctcgcag
     7621 gtgcgctggt tcgcggcacc atctcgatcg acgtcgaagc gtcggacaca acgagcgggc
     7681 tcggcggagt cgacgtcgtc gtcgatggaa cctcactcgg cgcctgcagc caatcgccgt
     7741 gccgcgtcgc gttcgatacg accacgctgc ccgacgggcc gttcgcgatt tctgcgtctg
     7801 cgactgacag ggctggcaat cgttctgtcg tcgcgagcat caccgcgatc gcggacaact
     7861 cagcgcctag cgatttcctc gtgagcccgc gctccggtga catcgtggct gactcgttca
     7921 cactgcagct cgcgattcac gatgcgagct tcgcgagcgt tcaatgcacg ctcgacggcg
     7981 cgacgatcgc cacgtcgacg aatccggcat tcaccacgac ggtcaccacc acgggcctcc
     8041 tcgacggcga cagcgtcgtt cgctgcgtcg cgacggacgg agccggcaac gtcggagtgt
     8101 cctcggcgac gattacgatt gctcgctgga cggctcggat ttcgccgacg acgctcaatc
     8161 tgcgttcgaa gggcggcgcg acgtcggtga ccgcggtgat cgagggccgc ggcacgcaga
     8221 gcctcaccgg tgtcaccgtg gcggtccccg gcggtagccc ggtcgcggca actccgatcg
     8281 cggtcggcga tgacgatcac gatggcatcc ccgatgtcgt gctgaagatc gatcggcaag
     8341 cgctcatcgc atcgagccgc ggagcactcg ccgccaatgc gatccaagcg ggtgtgccgt
     8401 tcaacgtga
//
LOCUS       DATLBZ010000695         3248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10336456, whole genome shotgun sequence.
ACCESSION   DATLBZ010000695 DATLBZ010000000
VERSION     DATLBZ010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10336456"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VMZ53_33945"
     CDS             complement(<1..1356)
                     /locus_tag="VMZ53_33945"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HUS33571.1"
                     /translation="MREIEDRLHAASSGRIEAEIVAIALEQTGAEHGALFVWDKEANG
                     LALVHHVVSGVTVTLPRVVTREGARAGIALHVYDTDEPYLVSDTSRDQLYTRYLIDVG
                     SIAAAPLSYQKRSIGVLTVSTREPRKLDQKALRAITAIAEVAAPLLRRVQVDKLSRAA
                     TGRPLMIKGLSSAWHEVERKIELAAPTNAPILIRGESGTGKDLVARAIHWNSKRAERP
                     YVTVNCAAIPETLLESILFGHVKGAFTGASFHKVGEFQKANGGTLFLDEVGELPVMLQ
                     AKVLRAVEQGEVQPLGSNEPPDRVDVRLICATNRDLEDMAKNALFRDDLFYRLSVMTL
                     ELPPLRSYKDNLEVLANVFLEQSATSHGKKAPRLGPDVLARMQAYDFPGNVRELKNTI
                     EHAVILCTGDELQVEDLPKPLQDRAPKNRRAQTQPQQTLDEAREKWLDPLERQYLTDL
                     LA"
     gene            complement(1366..2826)
                     /locus_tag="VMZ53_33950"
     CDS             complement(1366..2826)
                     /locus_tag="VMZ53_33950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33572.1"
                     /translation="MHVLIAVADVGAGVQLEEALNQAGFEAKWDGAQANGPRGAASPA
                     SPASILGHEVVVLDADHLGARLGAVADQWRDHPSVPGVVAVGTSAAAREQAPTARVTL
                     LSPTASVQTLANAIRDAAKLRLSTGMRWPVLRAALKLPPAQNTPEAWPATLLHARNAD
                     LELARTALRWHVQHYVTPTDTLEQMKAERCLSVPELETLAHADGTRTVQSLVKVGPLD
                     SPATARLLWALASMGAVTMTAEVRDAGTAGRRALEELRQHLKARSEKMARWTYFDVLE
                     ISPLAEYDEIEAAYRLVGARYSPRALQRFNLAELVALGKPIWELVEKARMTLVDHAAR
                     GRYVDWVRQNLPNLDTVWAIPEQQIRDAAEVFARGQQLLAQGDAHRAMGDFARACRNH
                     PGHPEYEANLAWVRFRVAVAGGKDQREAALAERREVEKYLLGRRPWARALVALALLCA
                     AGGDADAARWHLHISLQIDPNAPAAQQLAQRLGMRR"
     gene            2971..>3248
                     /locus_tag="VMZ53_33955"
     CDS             2971..>3248
                     /locus_tag="VMZ53_33955"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33573.1"
                     /translation="MTLLEIMIVLAILALVMGLIVGPRVIALFADSKVDLAKIAVQKY
                     ANEAYPQWAMRNPGKDCPERIEELSEISNQKSGHDPWGQPYKMLCPPNL"
BASE COUNT          525 a         1092 c         1086 g          545 t
ORIGIN      
        1 ggcgagcagg tcggtcaagt actggcgctc cagtggatcg agccacttct cgcgcgcttc
       61 gtcgagagtt tgctggggct gagtctgtgc gcgacggttc ttcggggcgc ggtcttgcag
      121 cggctttggg agatcctcga cttgcagctc gtcgccggtg cacaagatca ccgcgtgctc
      181 gatcgtgttc ttgagctcgc ggacgttgcc ggggaagtcg tacgcctgca tgcgcgcgag
      241 cacgtcgggc cccaggcgcg gcgccttctt gccgtgcgac gtcgcggatt gctcgaggaa
      301 cacgttcgcg agcacctcga ggttgtcctt gtagctgcgc agcggcggca gctcgagcgt
      361 catcacgctg aggcgataga acaggtcgtc gcgaaacagc gcgttcttcg ccatgtcctc
      421 gaggtcgcgg ttcgtcgcgc agatcaggcg gacgtcgacg cgatccggcg gctcgttgct
      481 gccgagcggt tggacctcac cttgctcgac cgcgcgcagg actttggcct gcagcatcac
      541 cggcagctcg ccaacctcgt cgagaaacag cgtgccgccg ttcgccttct ggaattcgcc
      601 gaccttgtga aagctcgcgc ccgtgaacgc gcccttcacg tggccaaaca agatcgattc
      661 gagcagcgtc tcgggaatcg ccgcgcagtt gaccgtgacg tacggccgct cggcgcgttt
      721 gctattccaa tggatagcgc gcgcgacgag atccttgccg gtgccactct cgccgcggat
      781 caagatcggc gcgttcgtcg gtgcggcgag ctcgatcttg cgctcgacct cgtgccacgc
      841 cgacgagagg cccttgatca tcagcggccg gcccgtggcg gcgcgcgaga gcttgtcgac
      901 ttgcacgcga cgcagcagcg gcgcggcgac ctcggcaatc gcggtgatcg cgcgcagcgc
      961 cttttgatcg agcttgcgcg gctcgcgcgt cgagaccgtg agcacgccga tcgaacgctt
     1021 ctggtagctg agcggcgctg cggcgatcga cccgacatcg atcaggtagc gggtgtacag
     1081 ctgatcgcgg gacgtatcgc tgacgaggta aggctcgtcg gtgtcgtaga cgtggagcgc
     1141 gatgccggcg cgcgcacctt cacgcgtgac gacgcgcggc aacgtgacgg tcacaccgga
     1201 aaccacgtga tgaacgagcg cgaggccgtt cgcttcttta tcccagacga acagcgcacc
     1261 gtgctcggcg ccagtttgct cgagcgcgat cgcaacgatc tcggcctcga tccggccgga
     1321 cgacgcggcg tgaaggcgat cctcgatctc gcgcacggtc gtagcttacc gcctcatgcc
     1381 gagtcgctgc gcgagctgct gcgcagctgg tgcattcggg tcgatctgca acgagatgtg
     1441 caggtgccag cgcgcggcat cggcgtctcc acccgccgca caaagcagcg ccagcgcgac
     1501 gagggcgcgt gcccacggac gccggccgag caggtacttc tcgacctcgc ggcgttcggc
     1561 gagcgcagct tcacgctgat ccttaccgcc ggcgacggcg acgcggaacc gcacccacgc
     1621 gaggtttgct tcgtattcgg gatggccggg atgattgcga cacgcccgcg cgaaatcgcc
     1681 catcgcacga tgcgcgtcac cttgcgcgag caattgctgg ccgcgcgcga acacctcggc
     1741 ggcgtcgcgg atctgctgct cgggaatcgc ccacaccgtg tcgagattcg gcaggttctg
     1801 ccgcacccaa tcgacgtagc gcccgcgcgc cgcatgatcg acgagcgtca tgcgtgcctt
     1861 ctcgacgagc tcccagatcg gcttgccgag tgccacgagc tccgcgagat tgaagcgctg
     1921 cagcgcacgc ggcgagtagc gcgcaccgac gagccgatat gcggcctcga tctcgtcgta
     1981 ctcggcgagc ggcgaaatct cgagcacgtc gaagtacgtc cagcgcgcca tcttctcgct
     2041 ccgcgccttc aaatgctggc gaagctcttc gagcgcgcgg cgtcccgcgg tcccagcgtc
     2101 gcgaacctcg gccgtcatcg ttacggcgcc catcgatgcg agtgcccaga ggaggcgcgc
     2161 cgtcgccggc gagtcgagcg ggccgacctt cacgagcgac tgcacggttc gcgtgccgtc
     2221 ggcatgcgcg agcgtctcga gctcgggcac gctgaggcag cgctccgcct tcatctgctc
     2281 gagcgtgtcg gtcggcgtga cgtaatgctg gacgtgccag cgcagcgcgg tacgggcgag
     2341 ctcgagatcc gcgttgcgtg cgtggaggag cgtcgccggc cacgcttcgg gcgtgttctg
     2401 cgcgggcggc aacttcagtg cggcgcgcaa caccggccag cgcatccccg tcgagaggcg
     2461 aagcttcgct gcatcgcgga tcgcattcgc gagcgtctgc acggacgcag tcggcgagag
     2521 cagcgtgacg cgcgccgttg gtgcttgctc gcgtgcggcc gccgatgtgc cgacggcgac
     2581 gacaccaggc accgacggat gatcgcgcca ctgatccgcg accgcgccga gcctcgcgcc
     2641 gagatgatcg gcgtcgagca cgacgacctc gtgtccgaga atcgatgccg gcgatgcggg
     2701 cgacgcagca cctcgcggac cgttcgcctg cgcgccgtcc catttcgctt cgaagccggc
     2761 ctggttcaag gcctcctcga gctggacgcc ggccccgacg tcagccaccg cgatcaggac
     2821 gtgcacgtac ggcagcgtaa cacgcggcgt ctcgtcactt tggcaggtcg atctccgccg
     2881 acgacaacaa actgcgcgaa aggtctcgga ccggtcgttg gcatccgtct tgttaagccc
     2941 gatgacatgc gccgcaaccg tcaacgagga atgaccctgc tcgagatcat gatcgtcctc
     3001 gcgatcctcg cccttgtcat gggcctcatc gtcgggccgc gagtcatcgc gctgttcgcc
     3061 gactccaaag tcgacctcgc gaagatcgcg gtgcagaagt acgcgaacga ggcgtacccg
     3121 cagtgggcga tgcgcaatcc cggcaaggac tgcccggagc gaatcgagga gctcagcgag
     3181 atcagcaacc agaagagcgg ccacgatccg tggggccagc cctacaagat gctgtgcccg
     3241 ccgaacct
//
LOCUS       DATLBZ010000696         6359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8113966, whole genome shotgun sequence.
ACCESSION   DATLBZ010000696 DATLBZ010000000
VERSION     DATLBZ010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8113966"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VMZ53_33960"
     CDS             <1..1037
                     /locus_tag="VMZ53_33960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HUS33574.1"
                     /translation="ITLPRSERSRDDDDEGPSCPITTPSYEHYQGYIGPAPQGIDAPA
                     AWRRGVRGQGVWFADVEGGWNARHEDLPGDRITHAGGEYIEHPVWRSHGTAVLGEVVG
                     RDNGKGVVGIAPDVDRVFTSSIGGTKVANAIDTAAEALRPGDVLLIELQGKGPRRRFL
                     PVEFWDDCYDAIKAATDRGVIVIEAAGNGGEDLDHKAYKRKFDRGVRDSGAIMVGAGG
                     PARDGFDDRARLDFSNYGGRVDVQGWGRKVATLDYGDLQRCDDVDDDDDFTDRHYTDE
                     FSGTSSASPIVAGAAVLVQSYAKQRGGTLSPQAIRSLLRATGTPQTSDPAKQIGPRPD
                     LARALATFGE"
     gene            1034..1591
                     /locus_tag="VMZ53_33965"
     CDS             1034..1591
                     /locus_tag="VMZ53_33965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33575.1"
                     /translation="MKVALLIALGLVAIAARAASAGDAMQCTASGCRPVSSHKTGDIT
                     IEAGAGIAKTWLDLREDSASPLMAEVAGGMGVFATPVVAIGLRGHVTLGRGPTTITSL
                     GVDVKLDRGGLFFAASPAIASVTAPETDALAAALELRAGLDLGSVQIALAVTPMYAFA
                     NDGLDATAQLGAALRVGANVAIVLP"
     gene            complement(1588..2466)
                     /locus_tag="VMZ53_33970"
     CDS             complement(1588..2466)
                     /locus_tag="VMZ53_33970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33576.1"
                     /translation="MLAKALELGSCGVQWTPPEGAIGEHTDTKLVVTEDDGTVWTIEL
                     LPAGFAIGAGEEAALSDDIHDSARIAGSRVLEAKLGRIGEGRCARVFRQWRDRTFTGE
                     LIIPVAKGAIDLRVAGIDIDVVRAALDSVLENIEVTALPERTTEVALTGAGVACEPPL
                     RFLPVAAEGKGVLIRSGLDGWRRMIEIWRIGRHKLKGKDLHAELVEHANKSVEDWPNV
                     QTDTQAIDDFGVCLQVEQFVIFSDERGTEKQALFRWWIAGDQSLWRLSSVAPTGVERD
                     AMRRDLEQVQETFRRL"
     gene            complement(2510..4108)
                     /locus_tag="VMZ53_33975"
     CDS             complement(2510..4108)
                     /locus_tag="VMZ53_33975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33577.1"
                     /translation="MSGSDLSGAVIEDRYEIHERIAAGAMGVVYRGNRLKLDRAVAIK
                     VMHASLPAAMDGRKRFEREAKLMAKLEHPHCVSIIDYGLHGSKPYVVMELVRGRSLHD
                     LIVEQGRVEPARAVDVLRQVLSGLAHAHEQGIIHRDIKPANIMVTPKAPLGLHARILD
                     FGLARMLEASASMSNGVAVGTPSYMAPEQCRGEQLDARVDIYACGVVLFEMLTGKKPF
                     IEKDPISIIKKALEEPVPKMADVTPGDYGALQDVVERALAKSPGDRYPTAVAMAEALD
                     AALSGRVAPEATTEVPLAAIATKRADPEATRPLPSADVPITVGSSVRVKPEPPGSSIR
                     RELPRSRMRMVVLGGVVVVAAAAIALVVFRDRLFASEAPREKIVVVEDKAPPPAPAPA
                     PRDPADEIVASAVELADAGKTQAAIDSLVKARRVYPDSARLAHTLGKLYFAKMWWNDG
                     LANLRDAVKLDPTLRADPELIAIAARAFTTTPSYDHRLASFLLELDPEKTKAALEDIA
                     RARPNPDIRSRVQALVKRLRADDL"
     gene            complement(4143..>6359)
                     /locus_tag="VMZ53_33980"
     CDS             complement(4143..>6359)
                     /locus_tag="VMZ53_33980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUS33578.1"
                     /translation="EELEEKAHQLTEQKAEVEKKNRQVELARQELEEKAEQLALTSKY
                     KSQFLANMSHELRTPLNSLLILSRQLADNAEQNLTDKQIRYAETIRQAGTDLMTLINE
                     ILDLAKIESGTMAVEVGAVRLANLRDYVDQTFRQVAEEKGLQFSVELDDSLPGNMETD
                     DMRLRQVLRNLLSNAIKFTERGRVKLRITRSGPDVLAFSVADTGIGIPQDKQRLIFEP
                     FTQADGSTSRKYGGTGLGLAISREIAGLLGGDLRVDSVVGQGSTFTLYLPVQYKQREA
                     RAQKLEGQRIALAHPAPPQVIDLSQGDRLLSDAARIPSPTQARMDEQQPPILEPATLT
                     RAIPDDYENLEPGDRVLLVVEDDLTFAMTMLEMARASGFKGVVAMNGGSALELARSIK
                     PDAITLDLRLPDLDGWVLLDRLKHDGATRHIPVHIVSGLDEERRGLQCGAHGFLQKPV
                     SMEELKEGLASVKEFVEKRVKQLLVVEDDPVQSQAISELIGDGDVETTTVASGEMALQ
                     TLSQKPYDCVVLDLRLPGMSGFDLIERIKADPQHRRLPVIVYTGRELTPEDRQRLHGL
                     AQTVIIKDVTTMERLLDETALFLHRVEANLPERKQRVLRRLAKNDPQLAERMVLVVDD
                     DLRNIFALTSLLEGHKMNVLYAENGKRALAKLEENPGIDCVLMDIMMPEMDGYEALRR
                     IRERPEWKELPVIALTAKAMKGDREKCLEAGASDYITKPVDSDQLLSLLRVWLYQA"
BASE COUNT          988 a         2049 c         2201 g         1121 t
ORIGIN      
        1 aaatcacgct gccgcgcagc gagcgcagtc gcgacgacga cgacgagggg ccgagctgtc
       61 cgatcacgac gccgtcgtac gagcactatc agggttacat cggacccgca ccgcagggca
      121 tcgatgcacc cgcggcgtgg cggcgcggcg tgcgagggca gggcgtctgg tttgcggacg
      181 tcgagggcgg ctggaacgcg cggcacgagg acctgccggg cgatcgcatc acgcacgcgg
      241 gcggcgagta catcgagcat ccggtgtggc gctcgcacgg gacggccgtc ctcggcgagg
      301 tggtcgggcg ggacaacggc aaaggcgtgg tcggaatcgc gccggatgtc gatcgcgtgt
      361 tcacgtcgtc gatcggcggc acgaaagtcg cgaacgcgat cgataccgcg gcggaggcgc
      421 tgcgcccggg cgacgtcctg ctcatcgagc tgcaaggcaa ggggccacgt cggcggtttc
      481 tgcccgtcga gttctgggat gactgctacg acgcgatcaa ggctgcgacg gatcgcggcg
      541 tgattgtcat cgaggcagcg ggcaatggtg gcgaggacct cgaccacaag gcgtacaagc
      601 gcaagttcga tcgcggcgtg cgcgactcgg gcgcgatcat ggtcggagca ggtgggccgg
      661 cacgcgatgg attcgacgac cgagcgcggc tcgacttctc gaactatggc ggtcgcgtcg
      721 atgtgcaggg ctggggccgt aaggtcgcaa cactcgacta cggcgatctg cagcggtgcg
      781 acgacgtcga tgacgacgac gacttcaccg atcgccacta caccgacgag ttctcgggca
      841 cgtcgagcgc gtcgccgatc gttgcgggtg cggcggtgct cgtgcagagc tacgcgaagc
      901 agcgaggcgg tacgctgtcg ccgcaggcga tccgcagctt gctacgggcg accggcacgc
      961 cgcagacctc ggatccggcg aagcagatcg gtccgcgtcc cgatttggcg cgtgcgcttg
     1021 ctacattcgg cgagtgaagg tcgcgctgct catcgcgctg ggactcgtcg cgatcgccgc
     1081 gagggccgcg tcggccggtg atgcgatgca atgcacggca tcggggtgtc gcccggtctc
     1141 gtcgcacaag acgggtgaca tcacgatcga ggccggcgct gggatcgcga agacgtggct
     1201 cgatcttcgc gaagatagtg cgtcgccgct catggctgag gtggctggcg gcatgggcgt
     1261 cttcgcaacg ccggtcgtcg cgatcgggtt gcgcgggcac gtaacgctcg gccgcggccc
     1321 gacaacgatc acgagcctcg gtgtcgacgt gaagctcgat cgaggaggcc tcttcttcgc
     1381 ggcgtcgcca gcgatcgcat ccgtgacggc gcccgagacg gacgcgcttg cggccgcact
     1441 cgagctgcgt gcgggcctcg acctcggctc cgtgcaaatc gcgctcgcag tcacgccgat
     1501 gtacgcgttc gcgaacgatg gcctcgacgc caccgcgcaa ctcggcgctg cactgcgcgt
     1561 cggtgcgaac gtcgcgatcg tgctgcctta gagcctacga aacgtctctt gcacttgctc
     1621 gaggtcgcgc cgcatcgcat cgcgctcgac gcccgttggc gcgacgctcg ataggcgcca
     1681 gagcgactgg tcgcccgcga tccaccagcg gaacagcgcc tgcttctcgg tgccgcgctc
     1741 gtcggagaag atcacgaact gctcgacttg gaggcacacg ccgaagtcgt cgatcgcttg
     1801 cgtgtccgtt tggacgttcg gccagtcttc gacgctcttg ttcgcatgct cgacgagctc
     1861 ggcatggagg tccttgccct tgagcttgtg acggccgata cgccagatct cgatcatgcg
     1921 gcgccagccg tcgagaccgc tgcggatgag cacgcctttg ccttcggcgg cgaccggcag
     1981 gaagcggagc ggtggctcgc acgcgacgcc ggcgccggtg agcgcaacct cggtcgtgcg
     2041 ttcggggagc gcggtgacct cgatgttctc gaggacggag tcgagcgccg cgcgcaccac
     2101 atcgatatcg atgccggcca cccgcagatc gatcgcgccc ttcgcgacgg gaatgatgag
     2161 ctcgcccgtg aacgtgcggt cgcgccattg gcggaacacg cgcgcgcagc gcccttcgcc
     2221 gatgcggccg agcttcgctt cgaggacgcg cgacccggcg atgcgcgcgg aatcgtgaat
     2281 gtcgtcggac agcgccgcct cttcaccagc gccgatcgcg aacccggcgg gcaagagctc
     2341 gatcgtccag acagtgccat cgtcttcggt gacgacgagc ttggtgtcgg tgtgctcgcc
     2401 gatcgcgccc tcgggcggcg tccattggac accgcagcta ccgagctcga gtgctttggc
     2461 cagcacgatc gcagtctagg ccactttggg gcgaacccga agttccgcct cagagatcgt
     2521 cggcgcggag gcgtttgacg agggcctgca cgcgcgaccg gatgtctgga ttgggccgtg
     2581 cccgggcgat gtcttcgagg gcggccttcg tcttctcggg gtcgagctcg agcaagaagc
     2641 tcgcgaggcg gtggtcgtag ctcggcgtcg tcgtgaatgc gcgagcagcg atggcgatca
     2701 actctggatc ggcgcgcagc gtcgggtcga gcttcaccgc gtcgcggagg ttcgcgaggc
     2761 cgtcgttcca ccacatcttg gcgaagtaga gcttgccgag ggtgtgcgcg agccgggcgc
     2821 tgtccggata gacacgccgc gctttgacga gcgaatcgat cgccgcttgg gtcttgccgg
     2881 cgtctgcgag ctcgaccgcc gatgcaacga tctcatctgc cgggtcacgc ggggcgggtg
     2941 ctggtgccgg cggcggcgcc ttgtcttcga cgacaacgat cttctcgcgc ggcgcctcac
     3001 ttgcgaacag ccgatcgcgg aacacgacga gcgcaatggc agccgcggcg acgacgacca
     3061 ccccgccgag cacgaccatc cgcatccgag agcgcggaag ctcgcggcgg atcgagctgc
     3121 ccggtggctc gggcttcacg cgtaccgacg acccgacggt gatcgggaca tccgcagacg
     3181 gcaacggccg cgtcgcttcc ggatctgcgc gcttcgtcgc gatcgccgcg agcggaactt
     3241 ccgtggttgc ctcgggtgcg acgcggccgc tcaaggccgc atcgagcgct tcggccatcg
     3301 cgacggccgt tggatagcga tcgcccggcg acttcgcgag cgcgcgctcg acgacatcct
     3361 gcagcgcgcc gtagtcaccc ggcgtgacgt cggccatctt cggtaccggc tcttcgagcg
     3421 ccttcttgat aatcgagatc ggatccttct cgatgaacgg cttcttgccc gtcagcattt
     3481 cgaacagcac gacgccacac gcatagatat cgacgcgtgc atcgagctgc tcgccgcggc
     3541 actgctctgg cgccatgtag ctcggcgtgc cgactgcgac gccattgctc atgctcgcgc
     3601 ttgcttcgag catgcgggcg agcccgaaat cgaggatgcg agcgtgcagg ccgagcgggg
     3661 ccttcggcgt caccatgatg tttgccggct tgatgtcgcg atggatgatg ccttgctcgt
     3721 gcgcgtgcgc gagaccggac agcacctggc gcagcacgtc gacggcgcgg gcgggctcga
     3781 cgcggccttg ctcgacgatg aggtcgtgca gcgagcggcc ccggacgagc tccatcacga
     3841 cgtacggctt cgagccgtgc aggccgtagt cgatgatcga gacgcagtgc ggatgctcga
     3901 gcttggccat gagcttggct tcgcgctcga agcgcttgcg cccgtccatc gcggctggca
     3961 gcgacgcgtg catgaccttg atcgcgacgg cgcggtcgag cttcaggcga ttgccgcgat
     4021 agacgacgcc catcgctccc gccgcgatcc gctcgtggat ctcgtagcgg tcttcgatga
     4081 cggcgccgct gagatccgac cctgacacgg gcacaggata tcagcggcgc gatgtaggcc
     4141 gtctaggcct ggtacagcca cacgcgtagc agcgacagca actgatcgga atccacgggc
     4201 ttcgtgatgt agtcactcgc tcccgcttct aggcacttct cgcggtcgcc cttcatcgcc
     4261 ttggcggtga gtgcgatcac ggggagctcc ttccactcgg gacgctcgcg gatccggcgc
     4321 agtgcttcgt agccgtccat ctccggcatc atgatgtcca tgaggacgca gtcgatgccg
     4381 gggttttcct cgagcttcgc gagcgcgcgc ttgccgttct cggcgtagag cacgttcatc
     4441 ttgtgacctt cgaggagaga cgtcagcgcg aagatgttgc ggagatcgtc gtcgacgacg
     4501 agcaccatgc gttccgcgag ctgcggatcg ttcttcgcga gacggcggag cacccgctgc
     4561 ttgcgctcgg ggaggttggc ctccacgcgg tgcaagaaga gggcggtctc gtcgaggaga
     4621 cgctccatcg tcgtgacgtc cttgatgatg acggtctgag cgaggccgtg caggcgctgg
     4681 cgatcttcgg gcgtgagctc gcgacccgtg tacacgatca ccggcaggcg acggtgttgc
     4741 gggtctgcct tgatccgttc gatcaggtcg aagcccgaca tgcccggcag gcgcaggtcg
     4801 aggacgacgc agtcgtacgg cttctgcgag agcgtctgca gagccatctc gccgctggcg
     4861 accgtcgtgg tctcgacgtc accatcgccg atgagctcgc tgatggcctg gctctgaacc
     4921 gggtcatcct cgaccacgag cagctgcttc acgcgcttct ccacgaactc cttgacgctc
     4981 gcgaggcctt ccttgagctc ttccatggag acgggcttct ggaggaagcc gtgagcaccg
     5041 cactgcaggc cgcggcgttc ctcgtcgaga cccgacacga tgtgcaccgg aatgtggcgc
     5101 gtcgcgccgt catgcttcag ccggtcgagc agcacccaac cgtcgaggtc ggggagccgc
     5161 aggtcgagcg tgatcgcatc cggcttgatc gagcgcgcga gctcgagggc cgagccgccg
     5221 ttcatcgcga cgacaccctt gaagcccgac gcgcgggcca tctcgagcat cgtcatcgca
     5281 aacgtcagat cgtcctcgac gacgagcagg acgcggtcac cgggctccag gttctcgtag
     5341 tcgtccggga tcgctcgcgt gagcgtggcc ggctcgagaa tcggcggctg ctgctcgtcc
     5401 atccgcgctt gcgtcggcga cggaatgcga gcggcgtcgc tgagcaggcg gtcgccttgc
     5461 gagaggtcga tgacctgcgg cggtgccgga tgcgccagcg cgatgcgctg accttccagc
     5521 ttctgggcac gcgcctcgcg ctgcttgtac tgcaccggga ggtagagcgt gaaggtcgag
     5581 ccctggccga cgacggagtc gactcgcaga tcgccaccga ggaggcccgc gatctcgcga
     5641 gagatggcga ggccaagacc cgtgccgccg tacttgcggc tcgtcgaacc atccgcttgc
     5701 gtgaacggct cgaagatcag gcgctgcttg tcctgcggga tgccgatgcc ggtatccgcc
     5761 acggagaacg cgagcacgtc cgggcccgag cgcgtgatgc gcagcttgac gcgtccacgc
     5821 tcggtgaact tgatcgcgtt cgagaggagg ttacgcagca cctggcgcag gcgcatgtcg
     5881 tcggtctcca tgttgcccgg caagctgtcg tccagctcga cggagaactg cagacctttc
     5941 tcctcggcga cctggcggaa cgtctgatcg acgtagtcgc gcaggttcgc gaggcgcacc
     6001 gctccgactt cgaccgccat cgttccggac tcgatcttcg cgagatcgag aatctcgttg
     6061 atgagggtca tcaggtcggt accggcttgg cggatcgtct ccgcgtaccg gatctgcttg
     6121 tccgtcaggt tctgctcggc gttgtcggcg agctgacggc tgaggatgag cagcgagttg
     6181 agcggcgtgc gcagctcgtg cgacatgttc gcgaggaact ggctcttgta cttcgaggtg
     6241 agggcgagct gctccgcctt ctcttcgagc tcctgacgag cgagctcgac ttggcggttc
     6301 ttcttctcga cctcggcctt ttgctcggtg agctgatgcg ccttctcctc gagctcttc
//
LOCUS       DATLBZ010000697        11786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10374988, whole genome shotgun sequence.
ACCESSION   DATLBZ010000697 DATLBZ010000000
VERSION     DATLBZ010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10374988"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VMZ53_33985"
     CDS             <1..814
                     /locus_tag="VMZ53_33985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33579.1"
                     /translation="GSYQVLANMQVGWISADHLKLGSPPAAKTLDVATVRHIVEPPGS
                     GVDDKGTSYTDQNYWNFCAPGAVTALLSYFTTDVKTWAAATFAEPYGPHVSHTYWNSN
                     DTTSGYQALGRAYLMHIAMQSKPPSFTNAGLAHFASYPTTGANLSDSRDVLNWEASRH
                     STSYKTFFYAVVPTSGLTSSRLHSDITHDIYGGHAVLAAVDTDFLPNWNRSLGHSIAI
                     VGYDDTAGTYTFIDTCGKRCNGSAQATNGGVWKVSQTNMFKAISAWGTGYVR"
     gene            complement(811..1692)
                     /locus_tag="VMZ53_33990"
     CDS             complement(811..1692)
                     /locus_tag="VMZ53_33990"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HUS33580.1"
                     /translation="MSTATALLKTIRPHQWVKNVFVAAALVFSRHLGDHVYVLRTAAA
                     ILAFCLLSGAVYAFNDVRDVEEDRQHPAKKNRPIAAGALSERAALTWAAIFATTALAG
                     CALLSWKLMLFALAYFVQNLLYSVRLKHIAFVDVLVIANGFLLRVLAGAAAINVPASK
                     WLLLCTALLALFFGLGKRAHELAWAKRSGGKTETRAALAGYNIPVIRVVMLVLALATS
                     GAYLAYTLDPHTVEFFGTANLPYSAPFVVFGLFRFMSLALWWPKDDSPTEAMLKDPWC
                     LLDFLAATATVLYIIYG"
     gene            complement(1715..3802)
                     /locus_tag="VMZ53_33995"
     CDS             complement(1715..3802)
                     /locus_tag="VMZ53_33995"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HUS33581.1"
                     /translation="MASEESKDEKVPTAAVERDHGKKGGGIPRPRPKTPIGWAILFLL
                     AALLAWKAGIGVAILFLLFVSGAPLFTIMLAAAALGAYALTDGSDSLNLVASHRGFDS
                     EFDGMVEHVLNVGLGDQVQVMSTIPLFIYAGYVLAESGTADRLVRFANALLGWIPGGL
                     AIVTIATCALFTVFTGASGVTIIALGGVLMPALVRVGYPEKFSVGLIAGTGSVGLLFP
                     PALPLFIYGTVYGLTNLDPEGWDTRRFLFAGIVPGLLLLTMLSMVAISVAIYRKLPTQ
                     KFSVKELGESFLLALPELFVPFGVILGLAKGFGLPEIAALTVVYVIVLEVGISRLLVM
                     LGVKWGRPLDPLVLWTTTREAMAMVGAIFIIIFASTALTNFMVTAKVPDLLVHWTQEH
                     VEQKWVFLLAINILLLVVGTVMDIFSAIVVVLPLIGPIAKAYGIDPYHLGVIFLLNLE
                     VGYLHPPVGLNLFITSVKFNRPITEVMWATMPFLATMIAALLIVTYVPALTEISVPEP
                     ERTGTVNNMIYMVHQQAEEGRAVKEVKIVDANGGELKDMKGQPIVKKLDDCNALTDET
                     KKTACQQVFIDVANCKVDPDVTKCSNKKIAEWAVGNLNAELSEHSIILVTEAPLVRPD
                     GTPVKDKAGAQVVKKSADCEKMSGFEKDTCMEVFLYASNCKINPPDDGNVDGCIAEKI
                     GEWVDSNMSEESP"
     gene            complement(3805..4425)
                     /locus_tag="VMZ53_34000"
     CDS             complement(3805..4425)
                     /locus_tag="VMZ53_34000"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HUS33582.1"
                     /translation="MSDSSVEGTPGGEAPSRASRLSIDLKPPTFPDDSAASGIIRKVD
                     GYIGIAEQIALFALLAAVVLTAAGAALSDKVLGDPFGRWWFTVVRGGTFTIAMLGAVF
                     ATHQQRHLAMDLVSRKIPPRGRLVLSIVLKLFTILIAYLLFKSGMHQRDHVGGTVKEF
                     IDDKTIVTMMPLGAALIILHSVLHIIIDVDYLARGKLPPEKARTGH"
     gene            complement(4529..5620)
                     /gene="dctP"
                     /locus_tag="VMZ53_34005"
     CDS             complement(4529..5620)
                     /gene="dctP"
                     /locus_tag="VMZ53_34005"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HUS33583.1"
                     /translation="MMKLTTLLSVAAAAVLATAPAARAENAEIRLATLAPSGSPWMEV
                     LNKAQAEIKDKTGGRVTLKYYEGGQQGDERDFVRKMGLGQLDGAAITSIGLAMIDESI
                     RVLELPMMFQSAEEMDYVADKMWPYFQKKFEKKGYKLNDRGEVGWIYFLSKGKVEKLA
                     DLKGQKLWMWGDDQLVGAMFKKLGLNGVPLGVPEVDAGLTSGKITACYGSPVAAVALQ
                     WYSKVKYMTSMPMSFAIGATVVKIDSLKKISAEDQKAVEEIGKASSKKLRKTIRKANE
                     DAKTTMTRKGITVVQTPVEMVSEFEKNAQEVWKDLTGKVYSKEELEMVLKYRDEYRAK
                     KGGKAATPAAPAKTAPAATPATPAAPAKK"
     gene            complement(5617..6549)
                     /locus_tag="VMZ53_34010"
     CDS             complement(5617..6549)
                     /locus_tag="VMZ53_34010"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HUS33584.1"
                     /translation="MRLLALAITTGLLAACSPQDLAINSTSKVLLAGQPSLQQEADYE
                     MARTAIPGALKTVEAFWISGYGNSGAKARLERILTEGYCQYGTAFVEDDWEEAKFRKD
                     IPAIEYHNARANHIFTRCMNYAMKSLGSGFQKDIFGTTDLAMKRIASTGGGNRFALLF
                     AGTSLGSLINHNLTRVEMISYLSTVEAIMLRVIEIDKKNGIPANKTHAALPYIALGML
                     YSGKPKSMGGEPEKAKAMFEQALAITDGKFLLARALMGYRVGLQTNDQKFFHEQLAKV
                     LETDPAIWPEQRLANEVAHRRARRYLSHEKELFQ"
     gene            6675..8249
                     /locus_tag="VMZ53_34015"
     CDS             6675..8249
                     /locus_tag="VMZ53_34015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUS33585.1"
                     /translation="MMPQRDVRNVVLASSLGTVFEWYDFYLYATLAPFFASLFFPKGN
                     DTAALLSAFATYAAGFLVRPFGALVFGRIGDLVGRKYTFLITIVVMGVATIAVGLMPT
                     YASVGIAAPIILVALRLLQGLALGGEYGGAATYVGEHAANDRRGYVTSWIQTTATLGF
                     FLSLLVIGLCRWQMSKAAFAEWGWRLPFLVSALLLASSVWIRLKLDESPVFLKMKAEK
                     RGSSHPLRDSFLRYPNNKLVALALFGATAGQGVVWYTGQFYALFFLTITLKVEYTTAY
                     LLVGASLLISTPLFVVFGRLSDRIGRLKIILAGCLLAAIAYIPLFHALAHFANPALDE
                     FADHTKITVAGRNCEMHIFVFPGTTFSPCDKAQDFLTKAGLSFETFGPLYQEDVVVTI
                     GDKELRGYDEAAYKAALKDAGYPQGSAKDINYAMVLLILVVLVAFVTMVYGPIAAYLV
                     ELFPAKIRYTSMSLPYHLGNGWFGGMLPLVATAMVAASGDIYRGLWYPIAVAVLTFLV
                     GIAFLRETPKDFDIHA"
     gene            8279..9862
                     /locus_tag="VMZ53_34020"
     CDS             8279..9862
                     /locus_tag="VMZ53_34020"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS33586.1"
                     /translation="MRWPLLVLLVVAACDAQREPRHQRRDPGALVVAQASDVITLDPV
                     LATDSESIQVSELIFEGLVGWKVGTTDIEPRLAMGWGVSDNGLIWTFQLRPNVSFHDG
                     TPFDAQAVVFAFERLLDPAHPQSLGKAGNYWHGMLEHVSKVVATGPLTVEIHVDQKYA
                     PLLGNLAMYPMVSPSAVRKWGAQFSEHPVGTGPFGFQSWTKGARVVVKRFPAYWGPPA
                     GLNQIVFQVVADARQRLINLESGSVDLAMAILPDEQPFVELHPDLVLHHTPGNDISYL
                     ALNTQQPPFDDVRVRRAINYAVNKEPIVKLGYQGRAIAADTPLPPTMWGYHTPAMRYQ
                     YDPELAKSLLAAAEADGVFDPKRVYTLYAPSTPRPYIPQPERIARLIQASLEQVGVHT
                     ELNLLPYTEHRAAIQAGKHDMCVFGWVGDTGDPDNFLYVLLASKQANTEKGAQNVALY
                     QDADVDRMLLEAQAATTEQTRSPIYAAIQDKIASDAPWVPLAHSELVVAGRAEIENVR
                     LSPTGHPLYKLIKRRTEER"
     gene            9859..>11786
                     /locus_tag="VMZ53_34025"
     CDS             9859..>11786
                     /locus_tag="VMZ53_34025"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HUS33587.1"
                     /translation="MSEPPKPPGDGDKKRADTESPEIDRVDTPSPEIRRVDTPSPQIR
                     RVDTPSEPMRRINTPSGFIRRLDTPATGERRITGSLKRVITDRFARVPRPTPQPPPEL
                     RLRLRTKLVVAMALAALVPVAIVATIATGVILSSLEAGLREDADRQLTVGLNLILRSV
                     ERLGDECVQLSESSDLAAAMERPDRMEAWFARESAHVPSARLQIVDAKGALLFDRVIG
                     NAPKRFEGIGIVPNDPIVAAGQSWTRTVTLVAIGDQVVVRAVSPIVDASLSLRGVAVL
                     SVPLDADFADSIKGALSADVLIGGTSGVMAVTFRSALGRRAVPVELNANERNTALRRQ
                     HVLRDLDVRIEDSGRVRNYTMAATGLLDNVDRPVGVVAVAVDRGQLAATKALAIRSLV
                     IGGLGALAFAMLLALFWSRRLGAPIAELHRGAIAVSRGDLDHRIDIPGGDELTDLADA
                     FNQMTSTLKDNQARLAARMKEIVALHDAGRAVSSVIDLDQVSRKIVDAVARTFDVQLA
                     ALWLVEGSILRASAARARRADVSSTLATDAALDSAEALKPIAEHVREHREAVRLVFAA
                     GDKRFGDAARAAGLPGPFVGLPLERKNRVVGVLAVARGEGAREFSEADLNLLTTFADQ
                     AGAAVENAQLYQEVRGAS"
BASE COUNT         2036 a         3831 c         3790 g         2129 t
ORIGIN      
        1 cggttcctat caggtgctcg caaacatgca ggtcggctgg atctcggcag accacttgaa
       61 gctcggctcg ccgcctgctg caaagacgct cgacgtcgca accgttcgcc acatcgtcga
      121 gccaccgggc tcgggcgtcg acgacaaagg cacgtcgtac accgatcaga actactggaa
      181 cttctgcgcg ccgggcgccg tcaccgcgct gctctcgtac ttcacgaccg atgtgaagac
      241 atgggccgcc gcgacgttcg ccgagcccta cgggccgcac gtcagccaca cgtactggaa
      301 ttccaacgac acgacgagtg gctaccaagc gctcggtcgg gcgtacctca tgcacatcgc
      361 gatgcagagc aagccgccgt cattcacgaa cgcgggtctc gcgcacttcg cgagctatcc
      421 gacgaccggc gcaaacctca gcgactcgcg cgacgtcctc aactgggaag cgtctcgcca
      481 ctcgacgtcg tacaagacgt ttttctacgc ggtcgtcccc acgtcgggcc tgacgtcatc
      541 gcggctgcac tccgacatca cgcatgacat ctacggtggc cacgccgtgc tcgccgccgt
      601 cgacacggac ttcttaccga actggaaccg cagccttggc cactcgatcg cgatcgttgg
      661 ctacgacgac accgcgggca cgtacacgtt catcgacacg tgcggcaagc gctgtaacgg
      721 ctcggcgcaa gcgacgaacg gtggcgtctg gaaagtctcg cagacgaaca tgttcaaagc
      781 gatcagcgcg tgggggaccg gctacgtgcg ctagccgtaa atgatgtaca ggaccgtggc
      841 cgtggcggcg aggaagtcga gcaggcacca cggatccttc agcatcgctt ccgtcggcga
      901 gtcgtccttg ggccaccaaa gcgcaagcga catgaaccgg aagaggccga acacgacaaa
      961 cggagccgag tagggcaggt tcgcggtgcc gaagaactcg acggtgtgcg gatcgagcgt
     1021 gtacgccaaa tacgcgcccg acgtagccag cgcgaggacg agcatcacga cgcggatgac
     1081 cgggatgttg tagccagcga gcgctgcgcg cgtttcggtc ttgccgcccg agcgctttgc
     1141 ccacgcgagc tcgtgtgcgc gcttgccaag gccgaagaac agcgcgagca gcgccgtgca
     1201 gagcagcagc cacttcgatg caggcacgtt gatcgctgcg gcgccggcga gcacgcgcag
     1261 caggaagcca ttggcgatca cgagcacgtc gacaaacgcg atgtgcttca gcctcacgct
     1321 gtacaacaaa ttttgtacga agtacgcgag tgcgaacagc atgagcttcc acgacagcag
     1381 cgcgcagccg gcgagcgccg tcgttgcgaa gatggctgcc cacgtcagcg cggcacgctc
     1441 ggacagagcg cccgcagcga tcggccggtt cttcttcgcc ggatgctgcc gatcttcctc
     1501 gacgtcgcgc acgtcgttga acgcgtagac cgcgcccgac agcaggcaga acgcgaggat
     1561 cgcagcggct gtgcgcagga cgtagacgtg gtcgccgagg tggcgcgaga acacgagcgc
     1621 ggctgcaacg aagacgttct tcacccactg gtgtgggcgg atcgtcttga gaagcgcggt
     1681 cgccgtgctc acaggcggac cttacacgga ctgctcaggg cgattcttcg ctcatgttcg
     1741 aatcaaccca ctcgccgatc ttctcggcga tgcacccgtc gacgttgccg tcgtccggcg
     1801 ggttgatctt gcagttcgac gcatagagga agacctccat gcacgtgtcc ttctcgaagc
     1861 cgctcatctt ctcgcagtcg gcggacttct tgacgacctg agcgccggcc ttgtctttga
     1921 ccggtgtgcc atcgggccgc acgagcggcg cttcggtcac gaggatgatc gagtgctcgg
     1981 agagctcggc gttgagattg ccgacggccc actccgcgat cttcttgttc gagcacttcg
     2041 tgacgtctgg gtcgaccttg cagttcgcga cgtcgatgaa gacctgctgg cacgccgtct
     2101 tcttcgtctc gtcggtgagc gcgttgcaat cgtcgagctt cttgacgatg ggctgcccct
     2161 tcatgtcctt cagctcgccg ccattggcgt cgacgatctt gacttctttc accgctcgcc
     2221 cttcttcggc ttgctggtga accatgtaga tcatgttgtt caccgtgccc gtacgttccg
     2281 gctcggggac cgagatttct gtcaacgccg gcacgtaggt gacgatgagg agagccgcga
     2341 tcatcgtggc gaggaatggc atcgtcgccc acatcacttc ggtgatgggc ctattgaact
     2401 tcaccgacgt gatgaagagg ttcaggccga cgggcgggtg gaggtaaccg acctcgaggt
     2461 tcaagaggaa gatcacgccg aggtgatacg ggtcgatgcc gtacgccttc gcgatcggtc
     2521 cgatcagggg tagcacgacg acgatcgccg agaagatgtc catcaccgtg ccgacgacga
     2581 gcagcaggat gttgatcgcg agcaggaaca cccacttctg ttccacgtgc tcctgagtcc
     2641 agtggaccag gaggtccgga accttcgcgg tgaccatgaa gttcgtcagt gctgtcgatg
     2701 cgaagatgat gatgaagatc gcgccgacca tcgccatcgc ttcgcgcgtg gtggtccaca
     2761 gcacgagtgg atcgagcggg cggccccact tcacgccgag catgacgagg aggcgcgaga
     2821 tgccgacctc gagcacgatc acgtagacga cggtgagggc cgcgatctcg gggaggccga
     2881 agcccttcgc gagaccgagg atgacgccga atgggacgaa cagctcgggc agcgcgagca
     2941 ggaagctctc gccgagctcc ttcacggaga acttctgggt cggcagcttg cgatagatcg
     3001 cgaccgagat cgcgaccatc gagagcatcg tcagcaacag gaggccgggc acgatgccgg
     3061 cgaacaagaa gcggcgcgtg tcccagccct cgggatcgag gttcgtgagg ccgtagacgg
     3121 tgccgtagat gaacagcggc agcgcgggcg ggaagagcag accgacggag cccgtgccgg
     3181 cgatgaggcc gacgctgaac ttctcggggt agccgacgcg aacgagcgcg ggcatgagca
     3241 cgccgccgag cgcgatgatc gtgacgccgg acgcgccggt gaagacggtg aacagcgcgc
     3301 acgttgcgat ggtgacgatc gcgaggccgc cgggaatcca gccaaggagt gcgttcgcaa
     3361 aacggacgag gcgatctgcc gttcccgatt cggcgagcac gtagcccgcg tagatgaaca
     3421 gcgggatcgt cgacatcact tggacttgat cgcccaggcc gacgttgagc acgtgctcga
     3481 ccatgccgtc gaactcggag tcgaagccgc ggtggctggc gacaagattc agcgagtcgc
     3541 tgccgtcggt gagcgcgtag gcaccgagcg ccgccgctgc gagcatgatc gtgaacagcg
     3601 gtgcgccgct cacgaacagc aggaacagga tcgcgacgcc gatgccggcc ttccacgcga
     3661 gcagcgcggc cagcagaaac aggatcgccc agccgatcgg ggtcttggga cgcgggcgtg
     3721 gaatgccgcc gcccttcttg ccgtggtctc gttcgacggc cgcggtcggg actttttcgt
     3781 ctttggattc ttccgacgcc atgactagtg ccccgtcctt gccttctcgg gcggcagctt
     3841 tccgcgggcg agatagtcga cgtcgatgat gatgtggagg accgagtgca ggatgatgag
     3901 cgccgcaccg agcggcatca tcgtgacgat cgtcttgtcg tcgatgaatt ccttcaccgt
     3961 gccgccgacg tgatcgcgct ggtgcatgcc gctcttgaac agcaagtacg cgatcaggat
     4021 cgtgaagagc ttcagcacga tcgagagcac gagccgcccc cgcggcggga tcttgcggga
     4081 tacgagatcc atcgcgaggt gacgctgctg gtgcgtcgcg aacaccgcgc cgagcatcgc
     4141 gatcgtgaac gttccgccgc gcacgacggt gaaccaccac cggccgaacg gatcgcccag
     4201 caccttgtcg gagagcgctg cgcccgccgc ggtgagcacg acagccgcga gcaacgcgaa
     4261 cagcgcgatc tgctcggcga tgccgatgta gccatcgacc tttcggatga tcccggacgc
     4321 cgcactgtcg tcgggaaacg tcggtggttt gagatcgatg gacaagcggc tcgcgcgcga
     4381 cggcgcctcg ccacccggcg tcccctccac tgatgaatcg ctcacgtttg cgatggtagg
     4441 ccaaatgcgc ggcccgccag caatcacgac tgccgaaaat gcaaaatgcc gcgacattca
     4501 tcgcggcacc tgcagatcgt ttcagtgact acttcttcgc gggcgcagcc ggcgtcgcgg
     4561 gcgtcgcggc cggggccgtc ttcgccggag cagcaggcgt cgcggccttg ccacccttct
     4621 tcgcgcggta ctcgtcgcgg tacttgagca ccatctcgag ctcttccttc gagtagacct
     4681 tgccggtcag gtctttccag acttcctgcg cgttcttctc gaactccgag accatctcga
     4741 cgggcgtctg gacaaccgtg atgcccttgc gggtcatcgt ggtcttcgcg tcctcgttcg
     4801 ccttgcggat cgtcttgcgc agcttcttgc tgctcgcctt gccgatttcc tcgacggcct
     4861 tctggtcctc agccgagatc ttcttgagcg agtcgatctt cacgacggtc gcgccgatcg
     4921 cgaagctcat cggcatcgac gtcatgtact tgaccttcga gtaccactgc agcgccactg
     4981 ccgcgaccgg cgagccgtag cacgccgtga tcttgccgct ggtgaggccg gcgtcgactt
     5041 ccggcacgcc gagcggcacg ccgttgaggc cgagcttctt gaacatcgca ccgacgagct
     5101 ggtcatcgcc ccacatccag agcttctggc ccttgaggtc agcgagcttc tcgaccttgc
     5161 ccttcgagag gaagtagatc caaccgacct cgccgcggtc gttcagcttg tagcccttct
     5221 tctcgaactt cttctggaag tagggccaca tcttgtcggc gacatagtcc atctcctcgg
     5281 ccgactggaa catcatcggc agctcgagca cgcgaatcga ctcgtcgatc atcgcgagac
     5341 cgatcgaggt gatcgcagcg ccgtcgagct ggccgaggcc catcttgcga acgaagtcgc
     5401 gctcgtcacc ctgctgacca ccttcgtagt acttcagcgt gacgcgacca ccggtcttgt
     5461 ccttgatctc ggcctgcgcc ttgttgagga cttccatcca cgggctgccc gacggcgcga
     5521 gcgtggcgag acgaatctcg gcgttctccg cgcgagcggc aggagccgtc gccagaacgg
     5581 cggcagcagc gacggaaagc aacgtggtca gtttcatcat tggaagagct ccttctcgtg
     5641 cgagagataa cggcgcgccc gacgatgggc gacctcgttg gcaagacgct gctcgggcca
     5701 gatcgcggga tccgtctcga gaactttggc tagttgttcg tggaagaact tctggtcgtt
     5761 ggtctggagg ccaacgcggt agcccatgag cgcgcgggcg agcaggaact tgccgtcggt
     5821 gatcgcgagc gcttgctcga acatcgcctt ggccttctcg ggctcgccgc ccatcgactt
     5881 cggcttgccg ctgtagagca tgccgagcgc gatgtacggc agcgcagcgt gcgtcttgtt
     5941 cgccgggatg ccgttcttct tatcgatctc gatcacgcgg agcatgatcg cttcgaccgt
     6001 cgacaggtac gagatcatct cgacgcgcgt caggttgtga ttgatcagcg agccgagcga
     6061 cgtacccgcg aacagcagcg cgaagcggtt gccgcctccc gtgctggcga tgcgcttcat
     6121 cgcgaggtcg gtcgttccga agatgtcctt ctggaagccc gagccgagcg acttcatcgc
     6181 gtagttcatg cagcgcgtga agatgtggtt ggcgcgtgcg ttgtgatact cgatggccgg
     6241 gatgtccttg cggaacttcg cttcctccca gtcatcctcg acgaacgccg tgccgtactg
     6301 gcagtagccc tcggtgagga tccgctcgag ccgcgccttc gcgccgctgt tgccgtagcc
     6361 gctgatccag aacgcctcga cggtcttgag cgcgccggga atcgccgtcc gcgccatttc
     6421 gtagtcggct tcctgctgca ggctcggctg gccagctagg agaactttcg acgtggagtt
     6481 gatggcgagg tcttgcgggc tgcacgcagc gagcagacct gtagtgatag ctagagcaag
     6541 cagacgcatg gacggaggct ccccttcgac ggtgacgatg acaccagatt cacccggttc
     6601 gacacgaacc tgagggacgc taagtcagcg aaagccgaga gtcaatcgcc cggtcgggct
     6661 atgctccgcg gggcatgatg ccccagcggg acgttagaaa cgtagtcctc gcttcatcac
     6721 tcgggactgt cttcgagtgg tacgacttct atctctacgc gacgctggcg ccgtttttcg
     6781 cgtcgctgtt cttcccgaaa ggcaacgaca ccgccgcgct gctctcggcg tttgcgacgt
     6841 atgcggcggg cttcctcgtg cggccgttcg gcgcgctggt gttcgggcgc attggcgatc
     6901 tcgtcggtcg caagtacacg ttcctcatca cgatcgtggt catgggcgtc gcgacgatcg
     6961 cggtcgggct catgcccacg tacgcctcgg tcgggatcgc ggctccgatt attttggtcg
     7021 cgctgcgcct gctgcagggc ctcgcgctcg gtggcgagta cggcggcgcc gcaacctacg
     7081 tcggcgagca cgcggccaac gatcggcgcg gctatgtcac gagctggatc cagacgaccg
     7141 cgacgctcgg cttcttcttg tcgctgctcg taatcggact ttgccgctgg caaatgtcga
     7201 aagcggcgtt cgccgagtgg ggctggcgcc tcccgttctt ggtctcggcg ctgctgctgg
     7261 cgtcgagcgt gtggatccgg ctcaagctcg acgagtcgcc ggtcttcctc aagatgaaag
     7321 cggagaagcg cggcagctcg catccactgc gcgactcgtt cctgcgttat ccgaacaaca
     7381 agctcgtcgc gctcgcgctg ttcggtgcga ccgcagggca gggcgtcgtt tggtacaccg
     7441 gccagttcta cgcgctgttc ttcctgacga tcacattgaa ggtcgagtac acgaccgcgt
     7501 acctgctcgt cggtgcgtcg ctcttgatct cgacgccgct gttcgtcgtg ttcggcaggc
     7561 tctccgatcg catcggccgc ttgaagatca tcctcgcggg ctgcctgctc gcggcgatcg
     7621 cgtacatccc gctgttccac gcgctcgcgc actttgcgaa ccccgccctc gacgagttcg
     7681 ccgatcacac gaagatcacc gtcgcgggtc gcaactgcga gatgcacatc ttcgtgttcc
     7741 ccggaacgac gttctcgccg tgcgacaagg cgcaagactt cctgacgaaa gcagggctgt
     7801 cgttcgagac atttggtccg ctctatcaag aagacgtggt cgtaacgatc ggcgacaagg
     7861 agctgcgtgg ctatgacgag gctgcgtaca aggcggcgct caaagatgcg ggctatccgc
     7921 aggggagcgc gaaagacatc aactacgcga tggtgctgct catcctcgtc gtgctcgtcg
     7981 cattcgtgac gatggtctac ggtccgatcg cggcgtacct cgtcgagctg ttccccgcga
     8041 agattcgtta cacgtcgatg tcgctgccgt atcacctcgg caatggctgg tttggcggga
     8101 tgttgccgct cgtcgcgacg gcgatggtcg ctgcgtcggg cgacatctac cgcggcctct
     8161 ggtacccgat cgccgtggcg gtcctgacat tcctcgtcgg gatcgcgttc ttgcgcgaga
     8221 ctccgaagga cttcgacatc cacgcgtagc gccgagcccg agtgctacag tcgccccgat
     8281 gaggtggcct ctgctcgtgc tgctggtcgt cgctgcctgc gacgcgcaac gcgagcctcg
     8341 tcatcagcgc cgcgatccag gtgcgctcgt cgtcgcgcag gcgtctgatg tcatcacgct
     8401 cgatccggtg ctcgcgaccg acagcgaatc gatccaagtc agcgagctga tcttcgaggg
     8461 cctcgtcggt tggaaggtcg gcacgaccga catcgagccg cggctcgcga tggggtgggg
     8521 cgtctccgat aacggcctca tttggacgtt ccagctgcgg cccaatgtct cgtttcacga
     8581 cggcacgccg ttcgatgcgc aggcagtcgt attcgcgttc gagcgcctgc tcgatccagc
     8641 gcatccgcag tcgctcggca aggcgggcaa ctactggcac ggcatgctcg agcatgtctc
     8701 gaaggtcgtg gcgacaggcc cgctcaccgt cgagattcac gtcgatcaaa agtacgcgcc
     8761 gctgctcggc aacctcgcga tgtacccgat ggtgtcgccg agtgccgtgc gcaaatgggg
     8821 cgcacagttt tccgagcatc ccgttggtac cggcccgttc gggttccagt cgtggacgaa
     8881 gggcgcgcgc gtcgtcgtga agcggttccc cgcgtattgg ggaccgccgg ccgggctgaa
     8941 ccagatcgtg ttccaagtcg tcgccgacgc gcgccagcgg ctcatcaatc tcgagtcggg
     9001 ctctgtcgat cttgcgatgg cgatccttcc cgacgagcaa ccgttcgtcg agctgcatcc
     9061 cgatctcgtg ctccatcaca cgccgggcaa cgacatctcg tatctcgcgc tcaacacgca
     9121 gcagcctccg ttcgacgacg tgcgcgtgcg tcgcgcgatc aactacgccg tgaacaaaga
     9181 gccgatcgta aagctcggct atcaaggtcg cgcgatcgcc gccgacacgc cgctgccgcc
     9241 gacgatgtgg ggctatcaca cgccggcgat gcgctatcag tacgaccccg agctcgcgaa
     9301 gagcctgctc gctgccgccg aggccgatgg cgtgttcgat ccgaagcgcg tctacacgct
     9361 ctacgcgccg tcgacgccgc gcccgtacat cccgcaaccc gaacgcatcg cgcggctcat
     9421 ccaggcatcg ctcgagcaag tcggcgtgca caccgagctc aacctgcttc cttatacgga
     9481 gcaccgcgcg gcgatccagg cgggcaagca cgacatgtgc gtgttcggtt gggtcggcga
     9541 cacgggcgat ccggacaact tcctctacgt gctgctcgcg tcgaagcaag cgaacaccga
     9601 gaaaggcgcg cagaacgtcg cgctgtatca ggacgccgac gtcgatcgga tgctcctcga
     9661 ggcccaagca gcgacgacgg agcaaacgcg ctcgccgatc tacgcagcga ttcaagacaa
     9721 gatcgcgtcg gatgcgccgt gggtgccgct cgcgcactcg gagctcgtcg tcgcgggccg
     9781 cgcagagatc gagaacgtcc ggctctcgcc gacgggccat ccgctctaca agctgatcaa
     9841 gcgacggacg gaggagcggt gagcgagccg ccgaagccgc cgggcgacgg cgacaagaag
     9901 cgcgccgata ccgagtcgcc ggagatcgac cgcgtcgaca cgccatcgcc ggaaatccgt
     9961 cgcgtcgata cgccatcacc gcagatccgc cgcgtcgata cgccctcgga gccgatgcgt
    10021 cgcatcaaca cgccgtcggg cttcatccgt cgcctcgaca cgccggcaac gggcgaacga
    10081 cgcatcaccg gatcgctgaa gcgcgtcatc accgatcggt tcgcgcgcgt gccgaggcca
    10141 acgccgcagc caccgcccga gctgcggctg cgtctgcgca cgaagctcgt cgtcgcgatg
    10201 gcgctcgcag cgctcgtgcc cgtggcgatc gtcgcgacga ttgcgaccgg cgtgatcctg
    10261 tcgagcctcg aagcgggcct gcgcgaagat gcggaccgcc agctcacggt agggctcaac
    10321 ctcatcttgc gttccgtcga gcgcctcggc gacgagtgcg tgcagctgtc ggaatcgagc
    10381 gacctcgcag ccgcgatgga gcggccggac agaatggagg catggttcgc acgcgagagt
    10441 gcgcacgtgc cctctgcgcg gctgcagatt gtcgacgcga agggcgcgct gctgttcgac
    10501 cgcgtgatcg gcaacgcgcc aaagcgcttc gagggcatcg gcatcgtgcc aaacgatccg
    10561 atcgtcgcag cgggccagtc gtggacgcgc accgtcacgc tcgtcgcgat cggtgatcaa
    10621 gtcgtcgtgc gtgccgtctc accgatcgtc gacgcgagct tgtcgctgcg cggcgtcgcg
    10681 gtgctcagcg tgccgctcga tgccgacttc gcggacagca tcaagggcgc gctctccgcc
    10741 gacgtgctga tcggcggcac gagcggcgtg atggccgtga cgtttcgatc ggcgctcggg
    10801 cgtcgcgcgg tccccgtcga gctgaacgcg aacgagcgga acacggcgct tcgccggcag
    10861 cacgtgctgc gcgatctcga cgtgcgcatc gaggactccg gccgggttcg caactacacg
    10921 atggccgcga cgggcttgct cgataacgtc gatcggccgg tcggcgtggt cgcggtcgcg
    10981 gtcgatcgag gccagctggc cgcgacaaag gcgctcgcga tccgctcgct cgtcatcggc
    11041 gggctcggcg cgctcgcgtt cgcaatgctg ctcgcgctgt tctggtcgcg acgtctcggt
    11101 gcgccgatcg ccgagctcca ccgcggtgcg atcgccgtct cgcgcggtga cctcgatcat
    11161 cggatcgaca ttcccggtgg cgacgagctg accgacctcg ccgacgcgtt caaccagatg
    11221 acgtccacgc tcaaggacaa ccaagcccgc ctcgctgcgc gcatgaagga gatcgtcgcg
    11281 ctgcacgatg caggtcgcgc ggtgtcgtcg gtcatcgatc tcgatcaggt gtcgcgcaag
    11341 atcgtcgatg ccgtcgcgcg aacgttcgac gtgcagctcg ccgcgctgtg gctcgtcgag
    11401 ggcagcatcc tgcgcgcatc cgcagcccgc gcccgccgcg ccgacgtctc gagcacgctc
    11461 gccacagacg ccgcgctcga ttccgccgaa gcgctgaagc cgatcgccga gcacgtgcgc
    11521 gagcatcgcg aggcagtccg cctcgtgttc gccgcgggtg acaagcgctt cggcgatgcc
    11581 gcacgtgccg cgggtctgcc gggtccattc gtcggcctgc cgctcgagcg aaagaatcgc
    11641 gtcgtcggag tgcttgcggt tgctcgcggt gaaggcgcgc gcgagttctc cgaagccgac
    11701 ctcaacctcc tcacgacgtt cgccgatcaa gcgggcgccg ccgtcgagaa cgcgcagctc
    11761 tatcaagaag tccgcggcgc gagtga
//
LOCUS       DATLBZ010000698         9405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6579948, whole genome shotgun sequence.
ACCESSION   DATLBZ010000698 DATLBZ010000000
VERSION     DATLBZ010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6579948"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1453
                     /locus_tag="VMZ53_34030"
     CDS             53..1453
                     /locus_tag="VMZ53_34030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HUS33588.1"
                     /translation="MAVSAVSQKSIPKALFELALPIVGLNVLNVLMLAVDSALCGRLP
                     NSQQALAALTFSLQIVFLLMVAMLGLLVGTIALVARAYGGGDTKRLNHLLVQSTQLTA
                     IVGVFVGAIGAIAARQILELLGATPAAAELGAEYLRPLMIGTPFFYLGLLYAGILRGV
                     GNTRIPFFAAVFANVVNAILNYGLILGNLGMPALGVTGSAIGTVIAQFLNLVVLVVIL
                     RRETIPNLKLPLRPRAIDHGLAGELLRIGWPAALDMLILNAGFLTAMGMLGRIDEVTV
                     AAHGIGMRVQSLAFVPGLGIAQATSAMIGQALGGGNVERARKIALASLSMCVAVMTVL
                     AIALTLGAHPLVHIFDVTKDPLESYSVEWMYILGVAMLPAGANMAFIGLLQGSGATRI
                     SLRINFWTTLVIQVPVAWLLGFVLDLGAFGVWLSFPIAFFAKAALGYIEFKREKWAVT
                     GVRIPAHAAAAAPTEH"
     gene            1469..2149
                     /locus_tag="VMZ53_34035"
     CDS             1469..2149
                     /locus_tag="VMZ53_34035"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HUS33589.1"
                     /translation="MDAIEKLIDKHGPEAICRALEPMLTPERIARIDAVLAQRLASVA
                     AAVEDTYDPHNAAATIRTCEALGLQELHVIEPGARFSAAKGVTRGAHRWIDLHRWPTA
                     VEAVDELHARGFRVYATLPDAKVSIEDVDVGSPLAVIFGNEHDGVSQTAIAACDASIT
                     VPMYGFTESYNLSVTVGLAMGRLAQRRRDHLGGHGDLDDVRKARLRARWFALRIRGVA
                     GVLERAFS"
     gene            2282..4717
                     /locus_tag="VMZ53_34040"
     CDS             2282..4717
                     /locus_tag="VMZ53_34040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HUS33590.1"
                     /translation="MWARTDQGQPAPSGGRRPPIRTPKAGGRSNGAGKPPRPPRPIWI
                     SIIKWLAIAGLVGVAIVAATVALTFWMYGRDPNLPRIEKLADYHPKQVTTILDHNDNR
                     IGEIYTERRTFVTYEKIPPVVVDAFIAAEDNNYWKHSGIDYTGMVRAFFANLKSGKKA
                     QGASTITQQVVKNFLLTPERTFKRKIQEIILARRLEQSLTKQEIMTLYLNQIYFGGGR
                     YGVQEASRFYFGKDINDPKNPITPGEAALLASMPKEPEAFYKMLFDPKKAGRVKSRQQ
                     YVLDNMVKLGKLQQADAQKWINEPIRLVKDPFPFMSSAPEWTDVVRAELKNRQIDPDT
                     SGAVVRTTLDPGLQLTAEKALQNGLRAVDARKKLGRAKRHLEADKMELELAKAAKRLP
                     KSGPAAKETYEAIVTKVDDKELTVDLGKWVATLALGTDEDERYNPPGEDGKVKTGTER
                     FKVGDVVDVVLAGKADDKKVRFAPGPEGAVVIIDVKTRKVRALVGGYETKTSGFNRAT
                     MAKRQPGSSFKTIVYTTALDSGKYTPASKLNDAPEVFDKWRPKNYESGKFLGPILLRN
                     ALAKSINTISIHMTYDLKPETVVEMAHKMGITSELPKEMSIALGSGEVTPLEMTNAVA
                     TIAAGGVAMAPSFVEAINGKMLPPSQGTQVLRPEVAYVATNMFQSVVQEGTATKAKVL
                     NIPIAGKTGTSNESKDVWFIGFTPDYIIGVWIGNDDATPLGSKETGGTTAVPVFVEIM
                     KAMNQPAKQFPRPAHVVEKTIDKETGLLAPDGAPKGTTRQEVFVEGTEPTEIAPMPGE
                     VTEQTSVTGDYSD"
     gene            4714..5763
                     /locus_tag="VMZ53_34045"
     CDS             4714..5763
                     /locus_tag="VMZ53_34045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33591.1"
                     /translation="MSWLRTAALLVASTSLAHANDVSPPVSAPVQDDGPIHRMLADVK
                     ARLDAAIVARPPKLVPPKKVAIKWKLAKLGTVDLGAPLVALTGADLDGDGHGELYAVT
                     SREVIALALKGKKLDEIGRVAFAGDRTSPMPRDVVGTIVVDGNAVIASVSAFTRGMRV
                     TWKGSTAGKGLAGELGDAGFVVCPGEAAQLAPGRNYFGADEKTAVYGVRCKAGLVEPD
                     GHPLRLRAALGVATARLSVDVERCAAANLGCTPTGHFEYPNAGFAFDLADVERDGKPE
                     VIYAASGAPGDPDILRVITLGDDDKKHPRLKRQFAAGGVAGLAVVDLDGDGVDEVIAG
                     VRLVGASRVDLWRLE"
     gene            5772..7232
                     /locus_tag="VMZ53_34050"
     CDS             5772..7232
                     /locus_tag="VMZ53_34050"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUS33592.1"
                     /translation="MLVLLATTTVARGEGRPRYGGNVEATLLGAPSTLDPLAARTHAE
                     ITVVGLVFDTLYRIDGDGSVQPFVADGEPVYDEKKTTARIAIKRGIRLHDGTELSAAD
                     VAQSLERARQQAKWVLAPLIGVKQDGDAVVISLRAPVAELTTRLALPQLAITKAGKAP
                     SDRPIGSGPYLLDTIDRKAHKLTLRAFDDHFAGRPYVDSLVLAWYDTPDGEARRFETG
                     KSQVSARGAAAFAGGQPAYRSVDIEGPASLLLYVGFGKAHADVTGDRSFRKALDLALA
                     RGGLTGISSGERVVPARSPLPVEAGGSALDAASKAGDMDGARRELATAAKRVASLAQP
                     AKLKLEILVEDTRPDDREIAERVVLALDKLGIGAVITPLAGQTMRQRVVAGQCDLYIG
                     QLAEPVTPAGASLIWWDAVFAAGNDDWPVMQPQVDPAAAAKEFAVRLPIVPLMFRSVR
                     MWHPTNLRGLAFDGSGRPCYADLMLFGVPTASKGKP"
     gene            7229..8977
                     /locus_tag="VMZ53_34055"
     CDS             7229..8977
                     /locus_tag="VMZ53_34055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33593.1"
                     /translation="MKLRGRFTLTLALAALVPITVAAVVTTRVIAASYKNRYAADRAA
                     TQQTLGRELARLEKSVSEAAASLASHDNPFVGNMLLDYVKGSGELDAAAQRRLREQSD
                     PMRRGLSLDILTITGPDDVVQVSPHYRAKVGDVDKTVRERATAHGGAAYYVRDTIMTG
                     RTKETVLVTEAAQLSRLEGYTVALWVGRRVGDDLLAPLRTSGVDARIVLPDNTVVVPP
                     AGSDWAALAKKEPPIRVPLKDPNGQAVAYVEVAVSDGGLDNILRDITLASALLALGAL
                     GAVVLTGLLVARRTANDLDLLVEGSLAAARGDLEHRVPVRSKDEVGAVASAFNFMMED
                     LRTAKDKLVIAERIAAWQEIARRLAHEIKNPLTPIQMAMDTLRKSYKKQHPEFPEILE
                     ESTRTVLEEADRLKKIVAEFSDFARMPKPELARIDLNDVIRSALSLYQGGAVKIDQRL
                     AKDLPEIDGDKGQITQVMLNLVENARDAIGARPSDDGRITITTRKGEANDRALVIVED
                     NGPGVPGELKDKVFAPYFTTKQTKGGTGLGLAIVHRIVSDHGGRITISDARDRGARFT
                     IELPLRSGAALLASRI"
     gene            9017..>9405
                     /locus_tag="VMZ53_34060"
     CDS             9017..>9405
                     /locus_tag="VMZ53_34060"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUS33594.1"
                     /translation="MRRLQKTIEQIGWTKGVPRFSGQDTVARAFDAMSRETHDCVLIV
                     DEENGIDKLVGIFTSRDFVNRVAASRKDPSKLSLGEVMTPAPRTLRPRDGVAYAINWM
                     AVEGFRNVPIVDDAGVLQGVLTVWDVMR"
BASE COUNT         1655 a         2982 c         3194 g         1574 t
ORIGIN      
        1 cggtgattgc gtcgttcggc gcgacgaccg aatgacgtag gatggccact acgtggcggt
       61 atctgctgtt tcacagaagt cgattccgaa ggcgttgttc gagctcgcgc tgccgatcgt
      121 cggcttgaac gtgctcaacg tgctcatgct cgccgtcgat tcggcgttgt gcgggcggtt
      181 gccgaactcg cagcaagcgc tcgctgcgtt gacgttctcg ctccagatcg tgtttctgct
      241 gatggtcgcg atgttggggt tgctcgtcgg cacgattgct ctcgtcgcgc gcgcgtatgg
      301 cggtggcgat acgaagcggc tgaatcatct gctcgtgcag tcgacgcagc tgactgcgat
      361 tgtcggcgta tttgtgggag caattggtgc gatcgccgcg cgacagattc tggagctgct
      421 tggcgcgacg ccggcggcgg ccgagctcgg tgccgagtat ttgcggccct tgatgatcgg
      481 cacgccgttc ttctacttgg ggctcttgta cgccggcatt ctgcgcggcg ttggcaacac
      541 acgcattccg ttttttgctg cggtgtttgc gaacgtcgtg aacgcgatcc tcaattacgg
      601 attgattctg ggcaatctcg gcatgcccgc gctcggcgtg accggttcgg caatcgggac
      661 ggtgattgcg cagtttctga acctcgtcgt gctcgtcgtg attttgcgac gcgagacgat
      721 tccgaatctc aagctaccgc tgcgacctcg cgcgatcgat cacgggcttg cgggcgagct
      781 gcttcgcatc ggctggcctg cggcactcga catgctgatt ctcaacgcgg ggtttctgac
      841 cgcgatggga atgctcggac gcattgatga agtgacggtt gccgcgcatg ggatcgggat
      901 gcgcgttcag agtctcgcgt tcgtgccggg gctcggcatc gcgcaggcga cgtcggcgat
      961 gatcggacag gcgctcggcg gtgggaacgt cgagcgagct cggaagatcg cgctcgcgtc
     1021 gctctcgatg tgtgtcgcgg tcatgaccgt gctcgcgatc gcgctgacgc tcggagcgca
     1081 ccctctcgtt cacatcttcg acgtgacgaa ggatccgttg gagtcgtact cggtcgagtg
     1141 gatgtacatc ctcggcgtcg cgatgctgcc ggctggcgcg aacatggcgt tcatcgggct
     1201 cttgcagggc agcggcgcga cgcgtatcag cctgcgcatc aacttctgga cgacgttggt
     1261 gattcaggtt cccgttgcgt ggctactggg cttcgttctc gacctcgggg cgtttggcgt
     1321 gtggctgagc tttccgatcg cgttcttcgc gaaggcggcg cttggctaca tcgagttcaa
     1381 gcgtgaaaag tgggcggtta ccggcgtgcg gattcctgct cacgccgcgg ccgccgcgcc
     1441 caccgaacat tagttagact gtcgccgcgt ggacgcgatc gagaagttga tcgacaagca
     1501 tggccccgaa gccatctgtc gcgcgctcga gccgatgctc acgcccgagc gcatcgcgcg
     1561 tatcgatgcc gtgctcgcgc agcggctcgc gtcggttgcg gcggcggtcg aggatacgta
     1621 cgacccgcac aacgccgcgg cgacgattcg tacgtgcgaa gcgctcggcc tgcaggagct
     1681 gcacgtcatc gagcccggcg cgcggttttc tgcggcgaag ggcgtcacgc gcggggcgca
     1741 tcgctggatc gatctacatc gctggcccac ggcggtcgaa gccgtcgacg agctacacgc
     1801 gcgcggcttt cgagtgtacg cgacgctgcc cgacgcgaag gtctcgatcg aagatgtcga
     1861 cgtcggctcg ccactcgccg tgatcttcgg aaacgagcac gacggcgtct cgcagaccgc
     1921 gatcgcggcg tgcgatgcat cgatcaccgt gccgatgtac gggttcaccg agagctacaa
     1981 cttgtcggtg actgttgggc tcgcgatggg gcggctcgcg cagcggcgcc gcgaccacct
     2041 cggcgggcat ggtgatctcg acgacgttcg caaggctcgg ctccgcgcgc gctggttcgc
     2101 gctgcgcatc cgcggcgttg ccggcgttct cgaacgcgcc ttttcctgaa ccgtgtcagg
     2161 gcggccacgc cgaatgtggc aaatcggccg cgaggctggg atagtccatg ggcatgcggc
     2221 tttgtaggcg gtatccaagt tgcagaaacc acccggcatg catgcgggtg caacgctaaa
     2281 gatgtgggct cgtaccgatc aaggtcagcc cgcgccttca ggggggcggc gtccgccgat
     2341 tcgcacgccc aaggcgggtg gtcgcagcaa tggcgcggga aaaccgccgc ggccgccacg
     2401 gccgatctgg atctcgatca tcaagtggct cgcgatcgcg gggctcgtcg gcgtggcgat
     2461 cgtagcggcg acggtcgcgc tgacgttctg gatgtacggc cgcgatccga acctgccgcg
     2521 catcgagaag ctcgccgact accacccgaa gcaagtcacg acgatcctcg atcacaacga
     2581 caaccgcatc ggcgagatct acaccgagcg gcgcaccttc gtgacgtacg agaagattcc
     2641 gcctgtcgtc gtcgatgcct tcatcgcggc ggaagacaac aactactgga agcactccgg
     2701 catcgactac accggcatgg tccgcgcgtt cttcgcgaac ttgaagtccg gcaagaaggc
     2761 gcagggcgcg tcgacgatca cgcagcaggt cgtgaagaac ttcttgctca cgcccgagcg
     2821 cacgttcaag cgcaagatcc aggaaatcat tctcgcgcgc cgtctcgagc agtcgctgac
     2881 gaagcaagag atcatgacgc tgtacctgaa ccagatctat ttcggcggcg gtcggtacgg
     2941 cgtgcaggaa gcgtcgcgct tctacttcgg caaggacatc aacgatccga agaacccgat
     3001 cacgcccggc gaggcggcgc tgctcgcgtc gatgccgaag gagcccgagg ccttctacaa
     3061 gatgttgttc gatccgaaga aggcgggtcg cgtgaagtcg cgccagcagt acgtgctcga
     3121 caacatggtg aagctcggca agctgcagca agccgacgcg cagaagtgga tcaacgagcc
     3181 gattcgcctc gtgaaggatc cgttcccgtt catgagctcg gcgccggagt ggaccgatgt
     3241 cgtgcgcgcg gagctcaaga atcgccagat cgatccggat acgagcggcg ccgttgttcg
     3301 cacgacgctc gatccgggct tgcagctgac cgcggagaag gcgctgcaga acggcctgcg
     3361 tgcggtcgac gcgcgcaaga agctcggccg cgcgaagcgt catctcgaag cggacaagat
     3421 ggagctcgag ctcgcgaagg cggcgaagcg cctgccgaag agcgggcctg cggcgaagga
     3481 gacgtacgaa gcaatcgtca cgaaggtcga cgacaaagag ctcacggtcg atctcggcaa
     3541 gtgggtcgcg acgttggcgc tcggcacgga cgaagacgag cggtacaacc cgccgggcga
     3601 agacggcaag gtgaagaccg gcaccgagcg gttcaaggtc ggcgatgtcg tcgacgtcgt
     3661 gctcgccggc aaggccgatg acaagaaggt gcgcttcgcg cctgggcccg agggcgccgt
     3721 cgtgatcatc gatgtgaaga cgcgcaaggt gcgcgcgctc gtcggtggct acgagacgaa
     3781 gacgagcggc ttcaaccgcg cgacgatggc caagcggcag ccgggctcga gcttcaagac
     3841 gatcgtctac acgaccgctc tcgacagcgg aaagtacacg cccgccagca agctcaacga
     3901 cgcgcccgaa gtgttcgaca agtggcggcc caagaactac gagagcggca agttcctcgg
     3961 cccgatcttg ctgcgtaacg cgctcgcgaa atcgatcaac acgatctcga ttcacatgac
     4021 gtacgacctc aagcccgaga ccgtcgtcga gatggcgcac aagatgggca tcacgagcga
     4081 gctgccgaaa gagatgtcga tcgcgctcgg ctcgggtgaa gttacgccgc tcgagatgac
     4141 gaacgcggtt gcaacgatcg cggcgggcgg tgttgcgatg gcgccgagct tcgtcgaagc
     4201 gatcaacggc aagatgctgc cgccgagcca aggcacgcag gtccttcgcc ccgaggtcgc
     4261 gtacgtcgcg acgaacatgt tccagtcagt cgtgcaggaa ggcaccgcga ccaaggcaaa
     4321 ggtgctgaac atcccgatcg cgggcaagac cggcacgtcg aacgaatcga aggacgtgtg
     4381 gttcatcggc ttcacgcccg actacatcat cggcgtgtgg atcggtaacg acgacgccac
     4441 gccgctcggc agcaaagaga cgggtggtac gacagcagtc cccgtgttcg tcgagatcat
     4501 gaaggcgatg aatcaacccg cgaagcagtt cccgcgcccg gcgcacgtcg tcgagaagac
     4561 gatcgacaaa gagacgggct tgctcgcgcc cgacggcgct ccgaaaggaa cgacgcgcca
     4621 ggaagtcttc gtcgagggca ccgagccgac ggagatcgcg ccgatgccgg gcgaagtgac
     4681 cgagcaaacg tcggtgaccg gagactacag cgattgagct ggctgcggac ggcggccttg
     4741 ctcgtagctt caacgtcgct cgcgcatgcg aacgacgtct cgccaccggt ttctgcaccc
     4801 gtgcaggacg acggcccgat ccaccgcatg ctcgcggacg tgaaagcacg actcgatgcg
     4861 gcgatcgtcg cgcgtccgcc aaagctcgtg ccgccgaaga aggtcgcgat caagtggaag
     4921 ctcgcaaagc tcggcaccgt cgacctcggc gcgccgctcg tcgcgctgac cggcgcagac
     4981 ctcgacggtg atggtcacgg cgagctctac gccgtcacgt cgcgcgaggt gatcgcgctc
     5041 gcgctcaagg gcaagaagct cgacgagatc ggccgcgtcg cattcgcggg agaccgcaca
     5101 tcgccgatgc cgcgtgacgt cgtcggcacg atcgtcgtcg atggcaacgc ggtgatcgcg
     5161 agcgtatcgg cattcacgcg agggatgcgc gtcacgtgga agggctcgac agcaggcaag
     5221 ggcctcgcgg gtgagctggg cgacgccggc ttcgtcgtgt gtcccggtga ggccgcgcag
     5281 ctcgcgcccg gccgcaatta cttcggcgcc gacgagaaga ccgccgtcta cggcgtgcgc
     5341 tgcaaagccg ggctcgtcga gccggatggc catccgctgc ggctgcgtgc tgcgctgggc
     5401 gtcgcgacgg cgcggctctc cgtcgatgtc gagcggtgtg ccgctgcgaa cctcggctgc
     5461 acgccgaccg gtcacttcga gtatccaaac gcaggcttcg cattcgacct cgcggatgtc
     5521 gagcgcgacg gcaagcccga agtgatctac gcggcgtccg gtgcgcccgg cgatccagat
     5581 atcctgcgcg tcatcacgct gggcgacgac gacaagaagc atccgcgcct caagcggcag
     5641 ttcgctgcgg gcggcgtcgc aggcctcgcg gtcgtcgatc tcgacggcga tggagtggac
     5701 gaagtcatcg cgggtgtccg cctcgtcggg gcatcgcgcg tggatctctg gaggctcgaa
     5761 tgagagtcgc cgtgctcgtc ctgctggcga cgacgaccgt cgctcgtggc gaggggaggc
     5821 cgcggtatgg cggaaacgtc gaggcgacgc ttctgggagc gccgtcgaca ctggacccgc
     5881 tggcggcccg gacgcacgcc gagatcaccg tcgttggcct cgtcttcgac acgctctatc
     5941 gcatcgacgg cgacggcagc gtccaaccct ttgtcgccga tggcgagccc gtctacgacg
     6001 agaagaagac gaccgcgcgc atcgcgatca agcgcggcat tcgcctgcac gacggcacgg
     6061 agctgtcggc cgccgacgtc gcgcaatctc tcgagcgcgc gcggcagcaa gcgaagtggg
     6121 tgctcgcgcc gctcatcggc gtgaagcagg acggcgacgc ggtcgtgatc tccttgcgcg
     6181 cgcccgttgc cgagctcacg acgcgcctcg cgctgccgca gctcgcgatc acgaaggcgg
     6241 gcaaggcgcc gagcgatcgg ccgatcggat ccgggccgta cttgctcgac acgatcgacc
     6301 gcaaggcgca caagctgacg ctgcgcgcgt tcgacgatca cttcgccggg cggccgtatg
     6361 tcgattcgct cgtgctcgct tggtacgaca cgcccgatgg agaggcgcgg cgcttcgaga
     6421 ccggcaagtc gcaggtgtcg gcgcgcggtg ccgcggcctt tgccggcggg cagcctgcat
     6481 atcgcagcgt cgatatcgag ggacctgcgt cgctgctgtt gtacgtcggg tttggcaagg
     6541 cgcatgcgga tgtcacgggc gatcgatcgt ttcgcaaggc gctcgatctc gcgctcgcgc
     6601 gcggcggcct cacgggcatc agctcgggag agcgcgtggt gcccgcgcgg tcgccgctcc
     6661 ccgtcgaggc gggcggcagc gcgctcgatg cggcgagcaa ggcgggcgat atggatggcg
     6721 cgcgacgcga gctcgcaacg gccgcgaagc gcgtggcgtc actcgcgcag cctgcgaagc
     6781 tcaagctcga gatcctcgtc gaggatacgc ggcccgacga tcgcgagatc gctgagcgcg
     6841 tggtgctcgc actcgacaag ctcggcatcg gcgcggtcat cacaccactc gccgggcaaa
     6901 cgatgcgtca gcgcgtggtg gcagggcagt gcgacctcta tatcgggcag ctcgccgagc
     6961 cggtcacgcc ggcgggcgcg tcgctgattt ggtgggacgc cgtgttcgcc gcgggcaatg
     7021 acgactggcc ggtcatgcag ccgcaagtgg atcccgcggc ggcggcgaag gaatttgcgg
     7081 tgcggctgcc gattgtcccg ctcatgtttc gctcggtccg catgtggcac ccgacgaacc
     7141 tgcgcggtct cgcattcgat ggctcggggc ggccctgcta cgcggacctc atgctgttcg
     7201 gcgtgccgac cgcgagtaag ggcaagccat gaagctgcgg ggccgattca cgctgacgct
     7261 cgcgctcgcc gcgctcgtgc cgatcaccgt cgctgcggtc gtgacgacac gcgtgatcgc
     7321 agcgagctac aagaatcgct acgccgctga tcgcgcggcc acgcagcaga cgctcggtcg
     7381 cgagctcgca cgcctcgaga agagtgtcag cgaagcagcg gcatcgctcg cgagtcacga
     7441 caatccgttc gtcggcaaca tgctgctcga ttacgtgaag gggtccggcg agctcgatgc
     7501 cgccgcgcag cgccgccttc gcgagcaatc ggatccgatg cgccgcggct tatcgctcga
     7561 catcctcacg atcacgggcc ccgatgacgt cgtgcaggtc tcgccgcact accgcgcgaa
     7621 ggtcggcgac gtcgacaaga cggtccgcga gcgagccacc gcgcatggcg gcgccgcgta
     7681 ctacgtccgc gacacgatca tgacgggccg cacgaaggag acggtgctcg tcaccgaagc
     7741 cgcgcagctc tcgcgcctcg agggctatac ggtcgcgctg tgggtcggcc gtcgcgtcgg
     7801 cgatgatctg ctcgcgccgt tgcgaacgtc cggcgtcgat gcgcgcatcg tgctgcccga
     7861 caacaccgtc gtcgtgcctc ctgcgggcag cgactgggct gcgctcgcga agaaggaacc
     7921 gccgattcgc gtgccgctca aggatccgaa cggccaagcg gtcgcgtatg tggaagtcgc
     7981 ggtctccgac ggcggactcg acaacatcct gcgcgacatc acactcgcat ccgcattgct
     8041 cgcgctcggc gcgctcggcg cggtcgtgct cacgggcctg ctcgtcgcgc gccgcacggc
     8101 gaacgatctc gatctgctcg tcgaaggctc gctcgccgcc gcgcgtggtg acctcgagca
     8161 ccgcgtgccc gtgcgctcga aagatgaagt cggcgccgtc gcgtcggcat tcaacttcat
     8221 gatggaagac ctccgcaccg cgaaagacaa gctcgtgatc gccgagcgca tcgcggcatg
     8281 gcaggagatc gcgcgacgcc tcgcgcacga gatcaagaat ccgctcacgc cgatccagat
     8341 ggcgatggat acgctgcgca agagctacaa gaagcagcac cccgagttcc cggagattct
     8401 cgaggagtcg actcgcaccg tcctcgaaga ggccgatcgc ttgaagaaga tcgtcgccga
     8461 gttctccgac ttcgcgcgca tgccgaagcc cgagctcgcg cgcatcgatc tcaacgacgt
     8521 catccgctcg gcgctatcgc tctatcaagg cggcgccgtg aagatcgacc agcgcctcgc
     8581 gaaggacctg cccgagatcg acggcgacaa gggccagatc acgcaggtca tgctcaatct
     8641 cgtcgagaac gcccgcgacg cgatcggcgc gcggccgagc gacgatggcc ggatcacgat
     8701 cacgacccgc aagggtgagg ccaacgaccg cgcgcttgtc atcgtggaag ataacggtcc
     8761 aggtgtacct ggcgagctga aggacaaggt cttcgcgccc tacttcacga cgaagcaaac
     8821 caagggcggc acaggacttg ggctcgcgat cgtgcatcgc atcgtgtccg atcacggggg
     8881 ccgcattacg atttccgacg cgcgtgacag gggcgctcgg ttcacgatcg agcttcctct
     8941 acgcagtggc gcggcactgc tagcctcgcg catctgagcc gggtaacctg gcttctgggc
     9001 ctgcattgga gggagcatgc gtcgactgca gaagaccatc gagcagatcg gctggacgaa
     9061 gggcgtgccg cggttctcgg ggcaagacac ggtagctcgc gcattcgatg cgatgagccg
     9121 cgagacgcac gactgcgtac tgatcgtcga cgaagaaaac ggaatcgata agctcgtcgg
     9181 catcttcacg agtcgtgact tcgtcaaccg cgtcgccgcg tctcgcaaag atccgtcgaa
     9241 gctctcgctt ggcgaggtca tgacacccgc gccgcgcacg ctgcgtcctc gcgatggcgt
     9301 tgcgtacgcg atcaactgga tggcggtcga aggcttccgc aacgtgccga tcgtcgatga
     9361 cgcgggcgtg ttgcaaggcg tgctcaccgt ctgggacgtg atgcg
//
LOCUS       DATLBZ010000699        13995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8165022, whole genome shotgun sequence.
ACCESSION   DATLBZ010000699 DATLBZ010000000
VERSION     DATLBZ010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8165022"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..1764
                     /locus_tag="VMZ53_34065"
     CDS             388..1764
                     /locus_tag="VMZ53_34065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUS33595.1"
                     /translation="MARVFLVNPPSPEPVKTPLLSFCHLAASLRAGGHDVALLDASAP
                     FAPKTHDEIRARVASFAPDLVGLHLKTLHVQPAYELAAALGDFALVGGGPHATIVPDE
                     PLSRGFRWVVRGEGEDALVELADLIDGKRAAASIDGLSWIDRGMVRHNPARAFISELD
                     RLAPPLSALDLFDPSWYGASERVPPAGLLSSRGCPAACTFCSNDVTGRKFRYRSASSV
                     AAEVKELGVAGLAFFDDSFAVGKRRVRELCGALKGSGVWWTCTAHPAHLDRDVLVEMQ
                     EAGCAGIDIGMESADPEMLVRIGKGVTVERVIDVLTWCADVGIHAVVNLMFGWPDETD
                     AELDATIGFIDRAIPIAGGFNARGVVVPYPGTKIYEQNHERFGFTGWWVREAPLAYPA
                     FPTSWDEAEIVRAYATDPALDRNFFQHPAHRVERIREALRLKAEATMIVTKRRSEPGR
                     GVLAAGAR"
     gene            1951..2754
                     /locus_tag="VMZ53_34070"
     CDS             1951..2754
                     /locus_tag="VMZ53_34070"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HUS33596.1"
                     /translation="MSNQRMPVAFLPHGGGPWPFVDLGWDLKDTPELKAYLESVRDLP
                     KTPPKALLVISAHWEEDVPTVMTSEKPPMLYDYYGFPPESYTIQWPAPGAPALAKRVQ
                     ELLGKAGFESRTDAQRGFDHGTFVPLKLTYPNADVPTIQLSLKAGLDPEEHLAIGRAL
                     APLRDEGVFIVGSGMTFHNLRAFRDPRSIPIAETFDAWLRDAATAPAAERDKHLVEWA
                     TAPAARAAHPREEHLLPLMVIAGAAGADKGQIAYNGTFAGLRLSAYHYG"
     gene            2776..5514
                     /locus_tag="VMZ53_34075"
     CDS             2776..5514
                     /locus_tag="VMZ53_34075"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUS33597.1"
                     /translation="MSTESEGPKQPPKADDKKKAEKEKDAKPAAAAAPAAAGPAPALD
                     ERKLKYGELLPHAGAYQPNDLLAMLQDGRAIVRGNAALALAASNQAAPELVQLVRDSE
                     QSVALAVAEAFAKLGPLARPLIPQIVQALDSAKPDVLDSMLDTLGQHVGKADDELTAA
                     LDVPFDLAMKTVVDACKRAGKGGVAMLAKATRHERSRVRVNAAGGLGKIGKTDPDTAL
                     AALNALEQGDPVPDVRTAAKQAILAVVAREKVEAVDALPKNIPDFEVRKLTLSELSEY
                     ADQIDVDQMLFALQDGRDHVRVNGARGLAAKGAKAARTATSIGLLLRDSVAAVRREAA
                     KAIGKLGPEGVAAAPELVGALGDAESDVVDAATDTLSAFGERAIDALVKGLEAGGEEH
                     GRRVAELITKSPRAADLLTEAFGSAAVNVQVNAAAGLGMLGKNGVGAKGLAALHGART
                     GGDVRTRAAVRAALDIIEAKGDTGPKEVSIAGFEDRFLAAADLDKAKAELERAGVADL
                     TAHLTDGRDVVRANAALALGALGAASAGSALPLGVLLRDDSPRVRLAAAQALDRIGDT
                     AVIETADYLVGALRDSDEKVAETVANVVRARKTRMISALVRGLETDDPRHAARIVEVI
                     NVLPDATEILCDAFESPAVNTQVNAALGLGMLGKDRVGKGRKALEGARTGGDARTREA
                     VRKALDTLDGPKAAGPQELTVEGFETKVLDAAAFGDVSKLRIDDLAHFLNDGRPVVRA
                     NAATALGAIGPTAVHTLSALGVLCRDDDMRVRIAAANAIDKLGDDAVREIAPFLVGAM
                     RGDAEVAKVVSRVLGARNAKVLGALVKGLETDDETQARRILELINALPDACEILCDAF
                     ESPAENVQVNAAMGIGMLGEKRAGSAGKKKLEGARTGGFARTREAVFKALAALKNG"
     gene            5527..6501
                     /locus_tag="VMZ53_34080"
     CDS             5527..6501
                     /locus_tag="VMZ53_34080"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HUS33598.1"
                     /translation="MSRWLVLAALAGCYAPASRVGSACDDVCPGNLECVDHVCVPPGT
                     SPGHDAAILDATIDAMVDARMIDGPPGDVDADGVADNVDNCPGKANADQHSEDGDALG
                     DVCDPCPHLSGNAADGDSDGVGDACDPQPGVAKQRIAFFDPFTSTRAEWELGSGASRV
                     GETLRVNGLTSFGDSILNVATGELRIETGGTVASISSTTPHQLSIGFGFLDSTHQKYH
                     YVELYNDAGTTGDIAISKANLGAYTGLATAQYPAPLPTGAWSMRIDESVAAQQIKLRA
                     KAGGVQYNELTAATNTALPNLATSTQATLYVQHADVRFDYWIVIETLP"
     gene            complement(6498..6989)
                     /locus_tag="VMZ53_34085"
     CDS             complement(6498..6989)
                     /locus_tag="VMZ53_34085"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HUS33599.1"
                     /translation="MHTSIAERLAFGTPLDPVADDTPIHLVPRAGTALIARILMSAIF
                     LTSGIAKLVDPAGAQGYMNMAGIPHADILVYVAGFAELLGGLALLLGFLTRIGALGLF
                     IFLIPTTLAFHGFWNMEGAEAKTQMVNFMKNLAIMGGLLAIYAWGPGRYSVDARIRRP
                     IQP"
     gene            7037..8377
                     /locus_tag="VMZ53_34090"
     CDS             7037..8377
                     /locus_tag="VMZ53_34090"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33600.1"
                     /translation="MEIGELLAGKYRVERVLGRGGQGEVVQATNLLLGQSVAMKFLLP
                     EMASDQVLVQRFLREARAAVQLKSEHVARVLDVGTLDSASPYIVFEYLDGTDLGQWNQ
                     SLTLGTIVDFALQATEALAEAHAKGIIHRDIKPGNLFITTASDGSPCLKVLDFGISKA
                     DFPGVTDLTASHTMLGTPRYASPEQLMSTSTADHRTDIWSLGVVLYELVEGRVPYDGE
                     TLTQLAMKVMTSPLPPITAQLPPAFAAVIERCLEKDPARRFQSMGELARALAPFASAP
                     EHAALLVRRASRISTPVFDGPITPSAAPSPVAAPRRHRWVIAVSMGVVVGAAAVVMLV
                     IGGRTKQVAAPKTTTIDGATEVHEMETVRQEAPADAQVIAVTPVDAQVIAETPVDAAV
                     DVDAGAQEQPHIAETPKQKPRKVKRKPIVKPVGKGSATTPAIVDRDGDGIPDVR"
     gene            8384..9325
                     /locus_tag="VMZ53_34095"
     CDS             8384..9325
                     /locus_tag="VMZ53_34095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33601.1"
                     /translation="MRFGVAFVLVCLVAGQVAAQPAAAQAEALFRQGKQLMDAGKTAE
                     ACKAFEASQKAEAAVSTLLNLANCREKNGELATAWGLFLDVARQTRAATDAAGKQFNA
                     TANQRASNIEHRLSHLTVGVSDNSKLPQLEVVRDGVAVDAGSWNRRLPIDGGKHTIVA
                     RAPGHKPWTITIDVASHDDDRQVEVPPLPEAEQAIETPTPRAETSRASSGHTGAWVLT
                     ATTLALAGGAIGFDLWARDTYDKTLVAGDPDLQTALWKSANRKRYVADGFAVGAIGCG
                     VAAIWLFARGGSGSTTTAHIEPVATPESTGFALVGGW"
     gene            9341..10105
                     /locus_tag="VMZ53_34100"
     CDS             9341..10105
                     /locus_tag="VMZ53_34100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33602.1"
                     /translation="MVGFRWCWVALVVAACGGFPAPTNNSDPLDDAGAGADGDDVDAP
                     ELMAGQDAFVYLDGAVIDGAPQGTAPTAGTASLSGTADDTKVLTATANGFNLGMPAGT
                     LHYVWQRCTTSACTSFSPIGTDKTTFTLTRSEGGQYVRVGVYAVNSCPSGCGQTATVY
                     SAVKGPVRRVDLFKGASCSVAGGCTSSACKIMEIRLVGFSSGSYATTCNASNTTNPFL
                     SYTTSIFPSERCCYGFAGKSTWATVSGIKSNTVTNW"
     gene            complement(10106..12097)
                     /gene="ligA"
                     /locus_tag="VMZ53_34105"
     CDS             complement(10106..12097)
                     /gene="ligA"
                     /locus_tag="VMZ53_34105"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HUS33603.1"
                     /translation="MPKSPSSRVSELVKLLRKYKDAYYNGSPLVSDAAYDALEDELRE
                     LEPGHDFLKTVGAPAPAVTSWEKARHAIPMGSLNKAVDEEEFRQWAQRCNDLGKKDKL
                     KDITNDLFVTEKLDGLSLAVTYDKGKLVDAITRGDGEVGERILANARRMKGVLPQLKS
                     KLATKGSITVRGEIILKLSDMKKAFPGAANPRNQASGTSKRFDGQGCEHLTVLFYDLD
                     GIDHKNEVEKFEILEELGFLVPNATACDLDGALKLHGEYAKKKRAALDYEIDGLVVRA
                     NDNHVQHMLGELGGRPRAAIAFKFASQAKVTTLIDIVWETGSSGRVSPVAVVKPVVLA
                     GATVQRASLHNAGMIAELDIGIGDEILVSRRNDVIPYVEEVVTKKGKTAKPPTKCPTC
                     SATLEKIGEYLACRNYKKCQALIEGRIENWIDAQGILEWGDKLINQLVEAKLVNEPAD
                     LYKLKPQQIANLERRGMIIAQKVLDNLRATLPLSLPKFLAALGIENFGLQTAKTICNA
                     GFDSLDKVQAATLAELSAIPGVGPAKGKAVFDGLKERKDEIKRLLAVGVVPVAKAQGG
                     PLAGLTFCFTGSLSKPRKELEQLVEERGGTLLAGVTKDLKYLVMADPGSGSSKAQKAA
                     KYGTECIDEAGFFALINKGGGKVAKPAKAGKSKAASATT"
     gene            12124..12819
                     /locus_tag="VMZ53_34110"
     CDS             12124..12819
                     /locus_tag="VMZ53_34110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33604.1"
                     /translation="MSQDELYAVTARVVDEFTSAAMLFTALDVSNAVKQTLTEARHRE
                     ISPIVRDIFERRGMGAYTQSQIDVMAAGNKPVKALLYHLPEQAPALYDDTMRSQLAVP
                     PQRSQRPAEEQFSASATEAGVRLGKDGRGRVPRALIENAGIRGDQVVVRADAATPKLT
                     IVAVGSTPAAAPIDPLYALPDLFFAPTDNGQPLAYEHPSLLHVPRSMLDIFGQDPNLV
                     ARIDGSSVVITRR"
     gene            12920..>13995
                     /locus_tag="VMZ53_34115"
     CDS             12920..>13995
                     /locus_tag="VMZ53_34115"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HUS33605.1"
                     /translation="MALVAIPASAQIQQIERTEALMLATGREDPMPVVEERLTVEIDG
                     ELAQTRLLQVVQNNTGGRVEGRYRLRAGTGSHVDGFAYWNGEQKIVGEVFEKQLANQV
                     YDAVTTRKRDPGLLQQDGEGAFSFKVFPIEAQEKKRVEVHWQKWLDRRGKIVHYRAPV
                     TRSDAAISITIAGNVKNVTSSTHRLTQEKITGGVRLRSDGARSAGEIQLDYEIDEPDW
                     QPDVYVHTAKGDHEGWFALSLAAPQEKATAVAAKDVTIVVDRSGSMTGEPMEHAIAAA
                     ADMVRMLNAKDRVNVIAFSDEVDPLFSQPHDADQQTKDQAIKFINALHAGGGTDIALA
                     LKTGIKSQEAKSENPKVIMFMTDG"
BASE COUNT         2402 a         4395 c         4823 g         2375 t
ORIGIN      
        1 cgctggcctc gacgacgacg tgaaggcgtg gaagctcgag agcgcgccgg atgcatttgc
       61 catcgtcgga cgccgcccgc agtaatacgc gggcgatagg cggggcgggc ctcgatgcga
      121 tccgagcgac ggctgcgacg actgcgacga ggcgccgccg cgcaggccgg ggcggcgagg
      181 cgcagcgcga tgcgatgtga tgcgatgcga cgactgcgac gaggcggcgc cgcgcaggcc
      241 ggggcggcga ggcgccgccg acgcggtgca ggccggcgcg gcgcggcgcg ggcgataggc
      301 ggcgcggcgc gggcgatagg cggcgcggcg tggggcgatt accgacgcac gtgtggcgga
      361 ttgcgggtag cgtagtagga ggcagtcatg gcgcgcgtct ttctcgtgaa tccgccatcg
      421 ccggagccgg tgaagacgcc gctgctgtcg ttttgtcatc tcgcggcatc gctgcgtgcg
      481 ggtgggcatg acgtggcgtt gctcgatgca tcggcaccgt ttgcgccgaa gacgcacgac
      541 gagattcgcg cgcgcgttgc gagctttgcg cccgatcttg tcgggctgca tttgaagacg
      601 ctgcacgtgc agcctgcgta cgagctcgct gcggcgctcg gcgacttcgc gctcgtgggc
      661 ggcgggccgc atgcgaccat cgtgcctgac gagccgctga gccgcgggtt tcggtgggtc
      721 gtgcgcggcg agggagaaga tgcgctcgtc gagctcgctg acctgatcga cggcaagcgg
      781 gcggcggcga gcatcgacgg gctgtcgtgg atcgaccgtg ggatggttcg gcacaatccg
      841 gcgcgcgcgt ttatcagcga gctcgatcga ttggcgccgc cgctttcggc gctcgatctg
      901 tttgatccga gttggtacgg agcgagcgag cgcgtgccgc cggcggggtt gctgtcgagc
      961 cgcgggtgtc ctgcggcctg tacgttttgt tcgaacgacg tgacggggcg gaagtttcgg
     1021 tatcgcagtg cgagctcggt ggcggcggag gtgaaggagc ttggcgttgc cgggctggcg
     1081 ttttttgatg attcgtttgc ggtggggaag cgacgcgtgc gcgagctgtg tggcgcgctg
     1141 aagggaagcg gcgtgtggtg gacgtgcacg gcgcatccgg cgcatttgga tcgcgatgtg
     1201 ctcgtcgaga tgcaggaggc gggttgtgcg ggcatcgaca ttgggatgga gagcgcggat
     1261 cccgagatgc tcgtgcgcat tggcaagggc gttacggtcg agcgcgtgat cgatgtgctc
     1321 acgtggtgcg cggacgtcgg cattcatgcg gtcgtcaatc tgatgtttgg ttggcccgac
     1381 gagacggatg cggagctcga cgcgaccatt gggtttatcg atcgcgcgat accgatcgcg
     1441 ggtgggttca atgcgcgcgg cgttgtcgtg ccttatccgg gcacgaagat ttacgagcag
     1501 aaccacgagc ggttcgggtt tacgggatgg tgggttcggg aggcgccgct cgcgtatccg
     1561 gcatttccga cgagctggga cgaagcggag atcgttcggg cgtatgcgac ggacccggcg
     1621 ctcgatcgga acttttttca gcatccggcg catcgcgtcg agcgcattcg agaagcgcta
     1681 cggctgaaag ccgaggccac gatgatcgtg acgaagaggc ggtccgagcc aggtcgcgga
     1741 gttttggcgg cgggcgcgcg ctgagccggc tgaaacagtg cgtttcgcat gggcacaggg
     1801 ctgtgcctcc ttgcaccggg ggcggtaggt caccaccatg ggccggtgac aaaggctccg
     1861 actcggaggc aggtgatcgg cgggctcgcg gctggcgcgg ggatggtgct gatcggtgcg
     1921 tgtgagtcgg aaaggacaaa ggagagagtc atgagcaacc agcgtatgcc cgtagcgttc
     1981 ctgcctcacg gcggcggtcc ctggccgttt gtcgacttgg gttgggatct gaaggacacg
     2041 cccgagctca aggcgtatct cgagagcgtt cgcgatctgc cgaagacgcc gccgaaggcg
     2101 ctgctcgtca tttcggcgca ctgggaagag gacgttccga cggtgatgac gtcggagaag
     2161 ccgcccatgc tgtacgacta ctatggcttt ccgccggaga gctacacgat ccagtggcct
     2221 gcgccaggcg cgccggcgct tgcgaagcgc gtgcaagagc tgctcgggaa ggcgggcttc
     2281 gagtcgcgca ccgacgcgca gcgcggcttc gatcacggca cgtttgtgcc tctcaagctc
     2341 acgtatccga acgcggatgt tccgacgatc cagctgtcgc tgaaggcggg gctcgatccc
     2401 gaggagcatt tggcgatcgg tcgggcgctg gcgccgctgc gtgatgaggg cgtgttcatc
     2461 gtcggcagcg gcatgacgtt ccacaacctg cgcgcgtttc gcgatcctcg atcgattccg
     2521 atcgcggaga ccttcgacgc gtggctgcga gacgctgcga ccgcgcctgc tgccgagcgc
     2581 gacaagcatc tcgtcgagtg ggcgaccgca ccggcggcga gggctgcgca tccgcgcgag
     2641 gagcacttgc tcccgctgat ggtgattgcg ggagctgcag gcgcagacaa ggggcagatc
     2701 gcctacaacg gcacgttcgc gggattgcgg ctgtcggcgt atcactacgg ctgatcgcgg
     2761 gtctacgatg cgtcggtgag caccgaatcg gaaggtccta agcagccccc gaaggccgac
     2821 gacaagaaga aggccgagaa ggaaaaggac gcgaagcctg ccgcggcggc cgcgcctgct
     2881 gctgccggac ccgctccggc gctcgacgag cgaaagctga agtacggcga gctgttgccg
     2941 catgccggcg cgtaccagcc gaacgacctg ctcgcgatgc tgcaagacgg tcgagcgatc
     3001 gtgcgtggca atgcagcgct cgcgctggcc gcgtcgaatc aggctgcgcc cgagctcgtg
     3061 cagctcgtgc gtgacagcga gcagtcggtt gcgctcgcgg tggccgaagc gttcgccaag
     3121 ctcggcccgc tcgcgcgacc gctgatcccg cagatcgtcc aggcgctcga cagcgcgaag
     3181 ccagacgtcc tcgattcgat gctcgacacg ctcggtcagc acgtgggtaa ggccgacgac
     3241 gagctcaccg ctgcgctcga cgtgccgttc gatctcgcga tgaagacggt cgtcgatgcg
     3301 tgcaagcgcg ccggcaaggg cggcgttgcg atgctcgcga aggcgacgcg tcacgagcgc
     3361 agccgcgttc gcgtcaacgc ggcaggtggc ctcggtaaga tcggcaagac cgatccagat
     3421 acggcgctcg cggcgctcaa tgccctggag caaggcgatc cggttcccga cgttcggacc
     3481 gccgcgaagc aagcgatcct cgcggtcgtg gcgcgcgaga aggtcgaggc cgtcgacgcg
     3541 ctgccgaaga acatccccga cttcgaggtg cgcaagctga cgctcagtga gctctcggag
     3601 tacgccgatc agatcgacgt cgatcaaatg ctcttcgcgc tgcaagacgg acgcgatcat
     3661 gttcgcgtca acggtgcgcg tgggcttgcg gcgaagggcg cgaaagcagc gcgcacggcg
     3721 acgtccatcg ggctcttgct gcgcgacagt gtcgcggccg ttcggcgcga ggcagcgaaa
     3781 gcgatcggca agctcggccc cgagggtgtt gccgcggcac cggagctcgt cggcgcgctc
     3841 ggtgatgccg agtcggatgt cgtcgatgcg gcgaccgata cgctgtcggc gtttggagag
     3901 cgcgcgatcg atgcgctcgt gaaaggcctc gaagcgggcg gtgaagagca cggccgtcgc
     3961 gtcgccgagc tgatcacgaa gtcgccgcgc gccgcggatc tcttgaccga agcgttcggc
     4021 agcgctgccg tcaacgtgca ggtcaacgcg gccgctggcc tcggcatgct cggcaagaat
     4081 ggcgtcggcg cgaagggcct cgccgcattg cacggtgcgc gcacgggcgg cgacgtgcgt
     4141 acgcgtgcag ccgtgcgcgc cgcgctcgat atcatcgagg cgaagggcga cacgggaccg
     4201 aaggaagtca gcatcgccgg cttcgaagat cggtttctcg cggcggcgga tctcgacaaa
     4261 gccaaggcgg agctcgaacg cgcaggcgtc gccgatctga cggcgcatct cacggatggc
     4321 cgcgatgtcg tacgggcaaa cgcggcactc gcgctcggcg cgctcggcgc agcatcagcg
     4381 ggctcggcac tgccactcgg cgtgctgctt cgcgatgact cgccgcgtgt ccggttggct
     4441 gccgcgcaag cgctcgatcg catcggcgac accgccgtga tcgagaccgc ggattatctc
     4501 gtcggtgcac tgcgcgattc cgacgagaag gtcgccgaga cagtggcgaa cgtggtgcgc
     4561 gcgcggaaga cgcgaatgat cagcgcgctc gtccgtgggc tcgagaccga cgacccgcgg
     4621 catgcagcgc gcatcgtcga ggtgatcaac gtgctgccgg atgcgacgga gattctgtgc
     4681 gacgcgttcg agagccccgc cgtgaacacg caggtcaatg ccgcgctcgg ccttggcatg
     4741 ctgggcaaag accgcgtcgg caagggccgc aaggcgctcg aaggtgcgcg caccggcggt
     4801 gatgcacgca cgcgcgaagc agtgcgcaag gcgctcgata cgctcgacgg tccaaaggcc
     4861 gcgggtccgc aggagctgac cgtcgaaggc ttcgagacaa aggtcctcga cgcggccgcg
     4921 ttcggtgacg tctccaagct gcgaatcgat gatctcgcgc acttcctcaa cgatggccgt
     4981 ccggtcgtgc gcgcgaacgc agcgactgca ctcggcgcga ttggtccgac ggcggtccac
     5041 acgctgagcg cactcggcgt gctctgccgg gacgacgaca tgcgcgttcg catcgcagcc
     5101 gccaacgcga tcgataagct cggcgacgat gctgtgcgtg aaatcgcgcc gttcctcgtc
     5161 ggagctatgc gcggcgatgc cgaggtcgcg aaggtagtga gtagagtgct cggcgcgcgc
     5221 aacgcgaagg tgctcggcgc gctcgtaaag ggtctcgaga cagacgacga gacgcaggcg
     5281 cgacgcattc tcgagctcat caatgcgctg cccgacgcgt gcgagatcct ctgcgacgcg
     5341 ttcgagagtc cggcggaaaa cgtgcaggtc aatgctgcga tgggaatcgg catgctcggc
     5401 gagaagcggg cagggagcgc cggcaagaag aagctcgaag gtgcgcgtac aggcggattt
     5461 gcgcgtactc gcgaggctgt tttcaaagcg ctagctgctc tgaagaacgg atgagacact
     5521 aggcgggtgt cacgctggct cgtccttgcg gccctcgccg ggtgttatgc cccggcgtcg
     5581 cgcgtcggca gcgcatgtga cgacgtttgc ccgggcaact tagagtgcgt cgatcacgtc
     5641 tgcgtgccgc cgggaacatc gcctggccac gatgccgcga tcctcgatgc gacgatcgac
     5701 gcgatggtcg acgcgaggat gatcgatggc ccgccgggag atgtcgacgc cgacggagtt
     5761 gccgacaacg tcgacaattg tccgggcaaa gcgaacgccg atcaacacag cgaagatggc
     5821 gacgcgctcg gcgatgtctg cgatccatgc ccgcacctaa gtggcaacgc cgcggacggc
     5881 gacagcgacg gtgtcggcga tgcgtgtgat ccacaacccg gcgtggcgaa gcagcggatc
     5941 gcattcttcg atccgttcac gtcgacgcgc gccgagtggg agctaggttc cggtgcatcg
     6001 cgcgtcggtg aaacgctgcg cgtcaatggc ctcacgagct tcggcgacag catcctgaac
     6061 gttgccaccg gcgagctgcg aatcgaaacc ggcggaaccg tcgcgagcat ctcgtccacc
     6121 acgccgcatc agctgtcgat cggcttcggc ttcctcgatt cgacgcacca gaagtaccac
     6181 tacgtcgagc tctacaacga cgctggcacg accggcgaca tcgcgatctc gaaggcgaac
     6241 ctcggcgcct atacgggcct cgcaacagcg cagtatccag caccgctgcc gacgggcgca
     6301 tggagcatgc gcatcgacga gtcggtcgca gcgcagcaga tcaagctccg cgcaaaggcc
     6361 ggcggcgttc agtacaacga gctcacggcg gcgacgaata cggctctgcc gaatctcgca
     6421 acgagcacgc aagcgacgct gtacgtccag catgccgacg ttcgcttcga ctactggatc
     6481 gtgatcgaga cgctgcctta gggctgtatc ggccggcgaa tgcgcgcgtc gacgctgtag
     6541 cggccgggtc cccacgcgta gatcgcgagg agcccaccca tgatggccag gttcttcatg
     6601 aagttgacca tctgcgtctt ggcctcggcg ccctccatgt tccagaaccc gtggaacgcg
     6661 agcgtcgtcg ggatcaagaa gatgaacaaa ccgagcgctc cgatgcgcgt gaggaagccg
     6721 agcagcaacg cgagaccgcc cagcagctcg gcgaagcctg cgacgtagac caggatgtcc
     6781 gcgtgtggaa tgcccgccat gttcatgtag ccctgcgcgc ccgcaggatc gacgagcttc
     6841 gcgatgccgc tcgtcaagaa gatggctgac atcaagattc gcgcgatgag agctgtgcca
     6901 gcgcgcggga cgagatggat cggtgtgtca tcggcgaccg ggtccagcgg tgtcccaaac
     6961 gccaacctct ctgcaatgga cgtgtgcatg ccctgtttcg tgcagcgtgc gtgcctacga
     7021 gttatcctcg agtgcggtgg aaatcggcga gctcttggcc ggcaagtatc gcgtcgagcg
     7081 cgtgcttggc cgcggtggtc aaggcgaggt ggtccaagcg acaaacctgc tgctcggcca
     7141 gtcggtcgcg atgaaattcc tgctgcccga gatggcgagc gaccaggtgc tcgttcagcg
     7201 cttcctgcgc gaggcgcgcg cagcggtcca gctcaagagc gagcatgtcg cgcgcgtgct
     7261 cgacgtcggc acgctcgaca gcgcatcgcc gtacatcgtg ttcgagtacc tcgacgggac
     7321 agacctcggt cagtggaatc agtcgctgac gctcgggacg atcgtcgact tcgcgctgca
     7381 agcgaccgag gcgctcgcgg aggcgcacgc gaagggcatc attcaccgcg acatcaaacc
     7441 ggggaacctc ttcatcacca cggcgagtga cggatcgcct tgcctcaaag tgctcgactt
     7501 cgggatctcg aaggcggact tcccaggcgt cacggacctg acggcgtcgc acacgatgct
     7561 cggcacgccg cgctacgcat cgcccgagca gctcatgtcg acgagcaccg ccgatcatcg
     7621 caccgacatt tggtcgctcg gcgtcgtcct ctacgagctc gtcgaagggc gcgtgccgta
     7681 tgacggcgag acgctcacgc agctcgcgat gaaggtcatg acatcgccgc tgcctccgat
     7741 caccgcgcag cttccgcccg cattcgcggc cgtgatcgag cgctgcctcg agaaggatcc
     7801 tgcgcggcgg ttccaatcga tgggcgagct cgcgcgtgcc ctcgcgccgt tcgccagtgc
     7861 gcctgaacat gcggcgttgc tcgtgcgccg cgcgagccgg atctcgacgc cggtgttcga
     7921 tggcccgatc acaccttcag ccgcaccatc gccggtcgct gccccccgcc gccatcgttg
     7981 ggtgatcgcc gtctcgatgg gcgttgtcgt cggcgcagcc gcagtcgtga tgctcgtgat
     8041 cggcgggcgc accaagcagg tcgctgcgcc taaaacgacg acgatcgacg gcgcgacgga
     8101 agtgcacgag atggagaccg tgcgtcagga ggctccggcc gatgcacagg tcatcgcggt
     8161 cacgcccgtc gatgcgcagg tgatcgcgga gacacccgtc gacgcggcgg tcgacgtcga
     8221 tgccggcgcc caagaacagc cgcacatcgc cgaaacgccg aagcagaagc cgcgcaaagt
     8281 gaagcgcaag cccatcgtga agccggtggg caagggatcc gcgacgacgc ccgcgatcgt
     8341 cgatcgtgac ggcgacggca ttcccgatgt acgctgaagg ctggtgaggt tcggggtagc
     8401 gtttgtcctc gtgtgcctgg tggctggcca ggtcgctgca caaccggcag cggcgcaggc
     8461 cgaggcgctg tttcgccagg gcaagcagct gatggacgcc ggcaagacgg ccgaggcatg
     8521 caaggccttc gaggcgagcc agaaggcaga ggccgcggtg tcgacgctgc tgaatcttgc
     8581 gaactgtcgc gagaagaacg gcgagctcgc gacggcgtgg ggcctgttcc tcgatgtcgc
     8641 gcggcagacg cgcgcagcga cggacgcggc gggcaagcaa ttcaatgcga ccgcgaatca
     8701 acgtgcgtcg aacatcgaac atcgactctc gcacttgacc gtcggcgtct ccgacaacag
     8761 caagttgccg cagctcgagg tcgtgcgcga tggcgtcgca gtggacgctg gatcgtggaa
     8821 tcgtcggctc ccgatcgacg gtggcaagca cacgatcgta gcgcgcgcac ctggccacaa
     8881 gccatggacg atcacgatcg acgtcgcgtc gcacgacgac gatcggcaag tcgaagtgcc
     8941 gccgcttcct gaagcggagc aggcgatcga aacaccgacg cctcgcgccg agacgtcgcg
     9001 cgcatcatcg ggtcacaccg gcgcgtgggt gctcaccgcg accacgctcg cgcttgccgg
     9061 cggcgcgatc ggcttcgacc tatgggcgcg cgatacctac gacaagacgc tcgtcgcggg
     9121 cgatccagat ctgcagacgg cgctctggaa atcggcgaac cgcaaacgct acgtcgcgga
     9181 tgggtttgcc gtaggagcga tcggctgcgg tgttgcggcg atctggctgt tcgctcgagg
     9241 cggcagcggc tcgacgacga cggcgcatat cgagcctgtc gcgacccccg agtcgacagg
     9301 ttttgcgctc gtcggaggct ggtgacggta tgatgggacc atggtcgggt ttcggtggtg
     9361 ctgggtcgcg ctcgtcgtgg ccgcgtgcgg cgggtttccc gcgcctacga acaactcgga
     9421 cccgctcgac gacgcaggcg caggcgccga cggtgacgac gtcgacgcac cagagctgat
     9481 ggctggccag gatgcgtttg tctatctcga cggcgccgtc atcgacggag cgccgcaggg
     9541 caccgcaccg acggccggca cggcgtcgct cagcggcacc gccgacgaca ccaaggtcct
     9601 caccgccacc gcgaatggct tcaacctcgg gatgcccgcc ggcacgcttc actatgtatg
     9661 gcaacgctgc acgacctccg cgtgtacctc gttctcgccg atcgggacgg acaagacgac
     9721 gttcacgctg acgcgttctg aaggcggcca atacgttcgc gtcggcgtct acgccgtgaa
     9781 ctcgtgccca tcgggatgcg gccagaccgc gaccgtgtat tcggcggtga agggtccggt
     9841 gcgccgcgtc gatctgttca agggcgcgtc atgcagtgtc gcgggtggct gcacgtcgag
     9901 cgcgtgcaag atcatggaga tcaggctcgt cggcttttcg tccggctcct acgcgacgac
     9961 ctgtaacgcg agcaacacga cgaatccgtt cctcagctac acgacgtcga tctttccgtc
    10021 ggagcgctgc tgctacggct ttgccggcaa gtcgacgtgg gcgacggtgt ccggcatcaa
    10081 gtcgaacacc gtcacgaact ggtagctacg tcgtcgccga tgcggccttg ctcttgccgg
    10141 cctttgcggg cttggcgacc ttcccgccgc ctttgttgat cagcgcgaag aagcccgctt
    10201 cgtcgatgca ctcggtgccg tacttcgcgg ccttctgcgc cttgctcgaa cccgagcccg
    10261 gatccgccat cacgaggtac ttcagatcct tcgtcacgcc ggcgagcagg gtgcccccgc
    10321 gttcctcgac gagctgctcg agctccttgc gcggcttcga cagcgatccg gtgaagcaga
    10381 acgtgagtcc ggcgagcgga ccgccttgcg ccttggccac cggcacgacg ccgacggcga
    10441 gcaagcgttt gatctcgtct ttgcgctcct tgagaccgtc gaacacggcc ttgcccttcg
    10501 cgggtccaac gcccggaatc gccgagagct cggcgagcgt cgcggcctgc accttgtcga
    10561 gcgaatcgaa gccagcgttg cagatcgtct tcgcggtctg gaggccgaag ttctcgatgc
    10621 cgagcgctgc gaggaatttc ggcagcgaga gcggcagcgt agcgcgcagg ttatcgagga
    10681 ccttctgcgc gataatcatg ccgcgccgct cgagattcgc gatctgctgc ggcttgagct
    10741 tgtagaggtc agcgggctcg ttgacgagct tcgcctcgac gagctggttg atcagcttgt
    10801 cgccccactc gaggatgcct tgcgcgtcga tccagttctc gatgcggcct tcgatcaggg
    10861 cctggcactt cttgtagttg cggcacgcga ggtactcgcc gatcttctcg agggtcgcgc
    10921 tgcacgtcgg gcactttgtc ggcggcttcg ccgtcttgcc cttcttcgtg acgacttctt
    10981 ccacgtacgg gatgacgtcg ttgcgccgcg acacgagaat ctcgtcgccg atgccgatgt
    11041 cgagctccgc gatcatgccg gcgttgtgaa gcgacgcgcg ctgcacggtt gcgcctgcga
    11101 gcacgaccgg cttgacgacg gccactggtg acacgcggcc cgacgagccg gtctcccaca
    11161 cgatgtcgat cagcgtcgtg accttcgcct gcgatgcgaa cttgaatgcg atcgcggcgc
    11221 gcggccggcc gccgagctcg ccgagcatgt gctggacgtg gttgtcgttc gcacgcacga
    11281 cgaggccatc gatctcgtag tcgagcgcgg cgcgcttctt cttcgcatat tcgccgtgca
    11341 gcttcagcgc gccatcgagg tcgcacgccg tcgcgttcgg tacgaggaag ccgagctcct
    11401 cgagaatctc gaacttctcg acctcgttct tgtggtcaat gccgtcgagg tcatagaaga
    11461 gcacggtcag gtgctcgcag ccttgtccgt cgaagcgctt gctcgtgccc gacgcctggt
    11521 tgcgcggatt cgcagcgccc ggaaacgctt tcttcatgtc cgagagcttg aggatgatct
    11581 cgccgcggac cgtgatcgaa cctttggtcg cgagcttgct cttgagctga ggaagcacgc
    11641 ctttcatgcg gcgcgcgttc gcaaggatgc gctcgccgac ttcgccgtcg ccgcgcgtga
    11701 tcgcatcgac gagcttgccc ttgtcgtacg tcaccgcgag cgataggccg tcgagcttct
    11761 cggtaacgaa caagtcgttc gtgatgtcct tgagcttgtc cttcttgccg aggtcgttgc
    11821 agcgctgcgc ccactgccga aactcttctt cgtcgacagc cttgttgagc gagcccatcg
    11881 gaatcgcgtg ccgcgctttc tcccacgacg tcacggcagg tgcgggcgcg ccgaccgtct
    11941 ttagaaagtc atggcccggc tcgagctcgc gcagctcgtc ttcgagtgcg tcgtacgcgg
    12001 cgtcgctgac gagcggcgag ccgttgtagt acgcgtcctt gtacttccgg agtaacttga
    12061 ccagctcact gaccctcgac gacggcgact tcggcataga atcacgatca cttgggggga
    12121 cagatgagtc aagacgagct gtacgcagtc actgcgcgag ttgtcgacga gttcacgagc
    12181 gccgcgatgc tgttcaccgc gctcgacgtg tcgaatgcag tcaaacaaac tctgacggag
    12241 gcgcgtcacc gcgaaatctc tccgatcgtt cgcgacatct tcgagcgcag ggggatgggc
    12301 gcctacacgc agagccagat cgacgtgatg gccgctggca acaagccggt gaaggcactg
    12361 ctctatcacc ttccggagca agcgccggcg ctctacgacg acacgatgcg gagccagctc
    12421 gccgtgccgc ctcagcgctc tcagcgtccg gcggaagaac aattctccgc atccgccacc
    12481 gaagccggcg ttcgcctcgg caaagacggt cgaggtcgcg ttccgcgcgc gctgatcgag
    12541 aatgctggga ttcgcggcga ccaagtcgtg gttcgtgctg atgcggcgac gccgaagctc
    12601 acgatcgtgg cggttggcag cacgcccgct gccgcgccga tcgatcctct gtacgcgctg
    12661 cccgatctgt tcttcgcgcc gaccgacaac ggccagccgc tcgcgtacga gcacccgtcg
    12721 ctgctccacg tgccgcgctc gatgctcgac attttcgggc aagacccgaa cctcgtcgcg
    12781 cgaatcgatg gctcgtctgt cgtgatcacg cggcgatagc cggtcggatc ggcggggcgg
    12841 gaaaccgaca ccgcgattag accgaacctt ggcgaggcgc gtttttcttc atatgcgcgt
    12901 cctctttgcc ggggccctcg tggccctcgt tgccattccc gcgtctgccc agatccagca
    12961 aatcgagcgc accgaagctc tgatgctcgc gacgggccgt gaagatccga tgccggtcgt
    13021 cgaagagcga ctcactgtcg agatcgacgg tgagctcgcg cagacccgcc tgctccaggt
    13081 ggtccagaac aatacgggtg gtcgcgtcga aggtcgctat cgcctccgag ccggcacggg
    13141 cagccacgtc gatggcttcg cgtactggaa cggagagcag aagatcgtcg gcgaggtgtt
    13201 cgagaagcag ctcgcgaatc aggtctacga cgcggtgacg acgcgcaagc gcgacccggg
    13261 cctccttcag caggacggcg agggcgcgtt ttcgttcaag gtgttcccga tcgaggcgca
    13321 ggagaagaag cgcgtcgagg ttcactggca gaagtggctc gatcgccgcg gcaagatcgt
    13381 tcactaccgc gcgccggtca cgcgctcgga cgcggccatc tcgatcacga tcgcgggcaa
    13441 cgtcaagaac gtcacgagct cgacgcatcg cctgacgcaa gagaagatca cgggtggcgt
    13501 tcggctccgc tcggacggcg cacgcagcgc gggcgagatc cagctcgact acgaaatcga
    13561 tgagccggac tggcagcccg acgtctacgt gcacaccgcg aaaggcgatc acgaaggttg
    13621 gttcgcgctc tcgctcgccg cgccgcagga gaaggcgacc gcggtcgctg cgaaggacgt
    13681 cacgatcgtc gtcgatcgct cgggctcgat gacgggcgag ccgatggagc acgcgattgc
    13741 ggccgccgcc gatatggtgc gcatgctgaa cgcgaaggat cgcgtcaacg tgatcgcatt
    13801 ctccgacgag gtcgacccgc tgttctcgca gccacacgac gccgatcagc agacgaagga
    13861 tcaggcgatc aagttcatca atgcgctgca cgccggcggc ggtacagaca tcgcgctcgc
    13921 gctcaagacc ggcatcaagt cgcaagaagc caagagcgag aatccgaagg tcatcatgtt
    13981 catgaccgac ggcca
//
LOCUS       DATLBZ010000700         9254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6587377, whole genome shotgun sequence.
ACCESSION   DATLBZ010000700 DATLBZ010000000
VERSION     DATLBZ010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6587377"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VMZ53_34120"
     CDS             <1..702
                     /locus_tag="VMZ53_34120"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HUS33606.1"
                     /translation="ERARDPNAKDVPAAPPKVALFEDKNRRVLDNIIDRNLETSLTKF
                     NGIQGDEVKRGDLGFGPGGGTGVGEGNGTGTTRGSKGKGTGGGGNAEGDFVSNKGKID
                     TGTERPGGGNCAKPPCGTAPKEVKIELKDPEGDFGGLTAEEINRVVKARQGVFRACYQ
                     TVLNRSPGLGGKLVIHFTIGGDGVVKSSKTAAGSSMHNADVESCVNSNIMRLKFPAKG
                     GLANVNYPFLFQPGG"
     gene            705..1568
                     /locus_tag="VMZ53_34125"
     CDS             705..1568
                     /locus_tag="VMZ53_34125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33607.1"
                     /translation="MRHVLFLAVILVGCNSNNEPQYVSCAPTGPAPMDTCRLDMLMMN
                     ADGELEAKGSLHVPVKPEDQWKQADRLRRMELQMALPDQSVEVPVYRLEHYDLEVEWT
                     VTNLDAMPGQFRVDLNGANEVFTYDPSLIVTDPSDDEAPPTPPLAGNIPIDIPANGTI
                     TGTFREDQLIEAAIDLDQVSRGNVNPFAAWLDVSKNKDEFQPLTAAMYDPATGETTYG
                     TPTGPPVPRLAFRQLVRVDIVFHPDRMMTLEYTLRVREHTEVIHPMGLNAPAGELQIL
                     DPAPFVPALGP"
     gene            1601..2170
                     /locus_tag="VMZ53_34130"
     CDS             1601..2170
                     /locus_tag="VMZ53_34130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33608.1"
                     /translation="MKRGLLLAVLPVLLHAASADARPASTGFFAEGGMGAVAFLPPQS
                     KDAAIGPVMTLRAGRDLFSWLSLGVYLAASSHEATVPPPPEGEWFQLYRGGGDLRVGG
                     LAGNVALFVEGGLGLSMVSSNILERTGRLDPGETFSVTFHAGAGLEYQLVNRHYAIGL
                     AVDGFLAPQFSTMKAIESRLYLRYTYGGG"
     gene            2163..3011
                     /locus_tag="VMZ53_34135"
     CDS             2163..3011
                     /locus_tag="VMZ53_34135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HUS33609.1"
                     /translation="MAEHSYLRVAHGGRWWKLLLELVLAPVWWVIGAGVVGIAVLSAT
                     GAKKLDDLDVVARLVVMHLSIAMMTPAALCAALVIGRRPGFLLSLAGRLRWRWLGRCV
                     GFAFAIQLAFIGFGVLVELIGSGSIDSLSHGGPRLWQVVAVTLLLVPFQASGEEVFNR
                     GTLQQIIGGWGAPAWVAIGVSTAAFVAMHGMPNVGTIAIAAMGISYAWLTLETGGLEA
                     AIAGHTMNNIVAFILAAAAHGTKALDPQELNERVGWASVVIQVVMIALYTVLVIRAWR
                     RSTQAG"
     gene            complement(3008..3637)
                     /locus_tag="VMZ53_34140"
     CDS             complement(3008..3637)
                     /locus_tag="VMZ53_34140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33610.1"
                     /translation="MRLAAALTVLVACEFRHGALPKQDDAARPADAPVDMVVLADART
                     DGSIDATPMCTTVACTSAGGTCKGGVCVIDSDLQTGVTCPAGMPCKVVCDTSIACQNG
                     GVKCGMATSCDVVCFGSSACQNGGVDCGQASTCNVSCVGPTACQNRASANNAGVECHA
                     SACTVTCNGSSACQDGIDVDTGGTCTSHCCGSTACQQGTDTCTNDSTCN"
     gene            complement(3656..6604)
                     /locus_tag="VMZ53_34145"
     CDS             complement(3656..6604)
                     /locus_tag="VMZ53_34145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33611.1"
                     /translation="MRSWVLLVAVLPACLAFDPAEDATQESRTNSKKCKAHKKHQDCG
                     TVTAPVSVEGGTFTADNGLTVTVPYGAVDSATTVTVSTTDIAAPGAQTPVYEFGPEGT
                     VFAAPLTITLPLPAGVTTGTVYWTKLGSTTEYEPIGGTIDPVAHTITAQTFHFSLGYV
                     GQPSGDRTVSGGGSRTYISATSRDDLPMDFASEDYDALVGDGSGGYARLCGRATGLGT
                     FSIDHVPNGDYILRDGKHYLVTSSPAPDLGFVLPGRPDLVPLTTDTNLELTLTGLSPW
                     NPGSDTELGDQIEWFSSEANVWDFNTERLTTLSGGENSVVIPINVADVDAGPASEIRS
                     AQGHRAAAAQLTVQHAGAANTPYLAITKVAQFPPDFDVLDGSTQQRTLALQSVPTTNT
                     LSIDYRGATWRSTLEQYGNPHHVLPSVSCPPVNCGAFVGALGQPYSANDGFYGANADL
                     LVMFDQSGTDIVSGPMAYADTSAFQGQWGVLYDVRASQRTVHKLPNTNARVGIGRFGF
                     IDSIEWTTTKAALEAGPILPPLLPPRNIRINGQNFFDDGGDLNGAATITWDPPASALQ
                     PAFYTINVVELYVDEDNNSHGVSVATIRTQNTSFTFPNAPSTSIGCDPSDPPAGPILK
                     PGHAYVFSVGAAASTSGSQVDAERIASAPFKETLNIATATVSSGLFGDVQGVPFAQLV
                     MDIENYPLGTAADASHVFWVEHGQAPWDPPTARNAGNVWMANLDGTDPRVIVSGQDLP
                     YEIVVANNKLYWTNRGDDSDSTVMSLDLATLDPNSSTNTPTLVASDPGASDILTYGGD
                     VYWISHAGTTRLSNGALSSVTWRAGVNFDTDGVNFYYALYGNGPPGPPTGTIESVPFA
                     GGPVSTLVSNQPQAWDVHTDGAYIYWSNQAWEQEGLATINRIPVGGGAIQTIVTGNEL
                     MKFFTLDANNIYYVRDNAAWAVPKSGGTPHAFAYVDTYGCPQADLTVAAGSLYFTDTC
                     GQATWRAPLP"
     gene            complement(6673..7998)
                     /locus_tag="VMZ53_34150"
     CDS             complement(6673..7998)
                     /locus_tag="VMZ53_34150"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HUS33612.1"
                     /translation="MSDERKWAERKVGFARLFARAKQVLTTDPTSSDEDDHVVDEEAA
                     RVLAAQAGRLKGGLAKVAQLAAYDPGASLGRGRGASTAARAVLGGLWDKAPSLSAGDI
                     SRVIEKDLGKAPQALFASWDATPIAAASLGQVHAATLRDGTHVVVKVQYPGVADALRA
                     DLDDESFIRKLAGAEIGRSLDDAALRALGDAVRDELDYAREAASQTAFAKAWDGDKSV
                     RIPRVIDELSSARVLTTTRARGKTVVDIAAGGAPDVRAQAAAAIYKFAWGSPLAHGLL
                     NADPNPGNFLIEVEDDGHVNVHCLDFGCALTLPDEVRESDREIWWGLLDEDPASAAER
                     FRMALARSGLLRRTDSLATNAHREWERALAAPLATHGTFHWDAKYAGDFAETTGRVLA
                     SGGLQLPAKQLLLWRQRLGVASVLGMLDATASFRPVLFDLIGSGKRALR"
     gene            complement(7995..>9254)
                     /locus_tag="VMZ53_34155"
     CDS             complement(7995..>9254)
                     /locus_tag="VMZ53_34155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HUS33613.1"
                     /translation="LWLLGHSWRQYLRQLALPGISSEASLMPIGVVLVLLYDPEQPLG
                     FMLLSLTYLLINFVFARLSRARHELQERVHDLEILNATGRRFSAALQLEELVEAVARE
                     ACKAIPEADAVTFIHRRAGRDSDLVMDGFDKRTDKFIRHTMAAGEGAAGWVIANATAR
                     RIDDLAEDAEIATDADHGIRSWLGVPLFMYGDCDGVVAVQSSRAAAFSPEHQRLLESL
                     ALQIASALQNAHLYELAMVDGLTALFVRRYFDARIEEEIERSRRYGSPFSVVMLDVDD
                     FKKLNDTYGHLVGDRVLRAIANVIKAQMRGVDTAARYGGEEMAVILPRTEMVGAYNLA
                     ERIREAIAELRITTDDDPPRALSVTASLGIAAFPESKAQSGEDLVRRADRALYRAKKT
                     GKNRVELFWTDDPSGAHRIADLAGGPG"
BASE COUNT         1548 a         3006 c         3074 g         1626 t
ORIGIN      
        1 gagcgcgctc gcgatccgaa cgcgaaggat gtcccagcgg cacctccgaa ggtcgcgctg
       61 ttcgaggaca agaaccgacg cgtgctcgac aacatcatcg atcgcaacct cgagacgagc
      121 ctgacgaagt tcaacggcat ccagggcgac gaagtgaagc gcggcgacct cggctttggt
      181 cccggtggcg gcacgggcgt cggcgaaggc aacggcaccg gcacgacgcg cggcagcaag
      241 ggcaagggca ccggcggcgg cggcaatgcc gagggcgact tcgtgagcaa caagggcaag
      301 atcgatacgg gcacggagcg ccccggtggc ggcaactgcg cgaagccgcc ctgcggtact
      361 gcgccgaagg aagtgaagat cgagctgaag gatcccgagg gcgacttcgg tggtctcacg
      421 gccgaggaga tcaaccgcgt cgtcaaggcg cgccaaggcg tgttccgcgc ttgctaccag
      481 accgtgctca atcggtcgcc gggcctcggc ggcaagctcg tcattcactt cacgatcggc
      541 ggcgacggcg tcgtgaagag cagcaagact gcggccggct cgtcgatgca caacgccgac
      601 gtcgagagct gcgtgaactc gaacatcatg cgcctcaagt tccccgcgaa aggcggactc
      661 gcaaacgtca actatccgtt cctcttccaa cccggaggct gatcatgcgg cacgtcctgt
      721 tcctcgccgt gattctcgtt ggctgcaaca gcaacaacga gccgcagtac gtcagctgtg
      781 caccgaccgg gcctgcaccg atggatacgt gccggctcga catgctgatg atgaacgcgg
      841 atggcgagct cgaagccaag ggcagcctcc acgtgccggt gaagcccgag gaccagtgga
      901 agcaagccga ccggttgcgg cgcatggagc tgcagatggc cctgcccgac caaagcgtcg
      961 aggtgccggt ctatcgcctc gagcactacg atctcgaagt cgagtggacc gtcacgaacc
     1021 tcgacgcgat gcctggccag ttccgcgtcg acctcaacgg cgcgaacgag gtcttcacct
     1081 acgatccgtc gctgatcgtc accgacccga gcgacgacga agcaccgccg acgccgccgc
     1141 tcgccggcaa catcccgatc gacatcccgg cgaacggcac catcaccggc acgttccgcg
     1201 aagatcagct catcgaagcc gcgatcgatc tcgatcaggt ttcgcgcggc aacgtgaatc
     1261 cgtttgcggc gtggctcgac gtcagcaaga acaaggacga gtttcagccg ctcacggctg
     1321 cgatgtacga tccggccacg ggcgagacga cgtacggcac gccaacgggc ccgcccgttc
     1381 cgcgcctcgc gtttcgtcag ctcgttcgcg tcgacatcgt gttccatccc gatcgcatga
     1441 tgacgctcga gtacacgctg cgcgttcgcg agcacaccga agtcatccac ccgatgggtc
     1501 tcaacgcgcc cgcaggtgag ctgcagatcc tggatcccgc accgttcgtg cccgcgctgg
     1561 ggccctagcc cacgcgattt gttttacgat tgctgggagc atgaaacggg ggttgctgct
     1621 ggccgtgctg ccggtcctgc ttcacgcagc atcggcggac gctcgtccgg catcgacggg
     1681 cttcttcgct gaaggcggca tgggcgcggt tgcgtttctg ccgccgcagt cgaaagacgc
     1741 cgcgatcgga cccgtgatga cgctgcgcgc ggggcgcgac ttgttctcgt ggctgtccct
     1801 gggcgtctac ctcgccgcgt cgagccacga agcgaccgtt ccgccacctc ccgaaggcga
     1861 gtggttccag ctctaccgcg gcggcggcga tctgcgcgtt ggcgggctcg ccggcaacgt
     1921 cgcgttgttc gtcgagggcg gccttggcct ctcgatggtc tcctcgaaca tcctcgagag
     1981 aaccggtcgg ctcgatccgg gcgagacgtt ctcggtcaca ttccatgcgg gcgccggcct
     2041 cgagtatcag ctcgtcaatc gtcactacgc gatcggcctt gcggtcgatg gcttcttagc
     2101 gccgcagttc tcgacgatga aggcgatcga atcgcgcctg tacctccgct acacgtacgg
     2161 cggtggctga acactcgtat ctgcgcgtcg cgcacggcgg acggtggtgg aagctactgc
     2221 tcgagctggt gctcgcgccc gtctggtggg tgatcggcgc cggtgtcgtc ggcatcgccg
     2281 tcctgagcgc gaccggtgcc aagaagctcg acgatctcga tgtcgtggcg cggcttgtgg
     2341 tcatgcacct ctcgatcgcg atgatgacgc cggctgcgct gtgcgccgca ctcgtgatcg
     2401 gacgacgtcc gggcttcctg ctctcgctcg cgggtcgcct ccggtggaga tggctcggac
     2461 gctgcgtcgg tttcgccttc gcaattcagc tcgcgttcat cggcttcggc gtcttggtcg
     2521 agctcatcgg atccggttcc atcgatagcc tctcgcatgg cgggccgcgc ctttggcagg
     2581 tcgtcgcagt gacgctcctg ctcgtgccct ttcaagccag cggcgaggaa gttttcaacc
     2641 gcggcacgct gcagcaaatc atcggcggtt ggggcgcgcc ggcgtgggtc gccatcggcg
     2701 tcagcacggc ggcattcgtc gcgatgcatg gcatgccgaa cgtcggcacg atcgccatcg
     2761 cagcgatggg catcagctac gcgtggctga cgctcgagac gggcggcctc gaagccgcga
     2821 tcgccggtca cacgatgaac aacatcgtcg cgttcatcct cgcagccgcg gcacacggca
     2881 cgaaggcgct cgatccacag gagctcaacg aacgcgtcgg ctgggcgagc gtcgtaatcc
     2941 aagtcgtgat gatcgcgctc tacaccgtgc tcgtgattcg cgcgtggcgc cgctcgacgc
     3001 aagccggcta gttacacgtg ctgtcgttcg tgcaggtatc ggtgccctgc tggcacgcgg
     3061 tcgacccgca acaatggctc gtgcacgtac cgcccgtatc gacgtcgatg ccgtcctgac
     3121 acgcactcga gccattgcac gtcacggtgc aggccgacgc gtgacactcg actccggcgt
     3181 tgttcgcgct ggctcggttc tgacacgccg tcggaccgac gcagctcacg ttgcacgtcg
     3241 acgcctgacc gcaatcgacg ccgccgttct gacatgcaga cgagccgaag cagacgacat
     3301 cgcatgaagt cgccatgccg cacttcacgc cgccgttctg gcacgcaatg ctcgtatcgc
     3361 aaacgacctt gcacggcatg ccggccggac atgtcacgcc ggtctgaaga tcgctgtcga
     3421 tcacgcagac gccgcctttg cacgttccgc cggcggacgt acaagcgacc gtggtgcaca
     3481 tcggcgttgc atcgatcgaa ccatcggttc gagcgtcggc gagcacgacc atatcgaccg
     3541 gcgcgtcagc gggcctcgcc gcatcgtctt gcttgggcag cgctccgtgc cggaactcgc
     3601 acgcaaccaa gacggtcagc gctgcggcga gcctcatcgc tacatcatag cgggctcatg
     3661 gcagcggcgc gcgccaggtc gcttgaccgc aggtatcggt gaaatatagc gagcctgcgg
     3721 cgacggtgag atcggcctgc gggcagccgt acgtgtccac atacgcaaac gcatgcgggg
     3781 ttccacccga cttcggcacc gcccacgcgg cgttgtcgcg cacgtagtag atgttgttcg
     3841 cgtcgagcgt gaagaacttc atcagctcgt tgccggtgac gatcgtctgg atcgcaccgc
     3901 caccaacggg gatgcgattg atcgttgcga ggccttcctg ctcccacgcc tgattcgacc
     3961 agtagatgta cgcaccgtcg gtgtgaacat cccaggcttg cggctggttg ctcaccaacg
     4021 tggagaccgg acctccggcg aatggcaccg actcgatcgt tccggtcggc ggacccggcg
     4081 gcccgttgcc gtagagcgcg tagtagaaat tcacgccgtc ggtgtcgaag ttgacgcctg
     4141 cgcgccacgt caccgacgag agcgcaccat tgctcaggcg cgtcgtgcca gcgtgagaga
     4201 tccaatacac gtcgccgccg tacgtgagaa tgtcgctcgc gcccggatca cttgcgacga
     4261 gcgtcggtgt gttcgtcgag ctgttcggat cgagcgttgc gagatcgagg ctcatgaccg
     4321 tgctgtccga gtcgtcgccg cggttcgtcc agtagagctt gttgttcgcg accacgatct
     4381 cgtacggcag atcttggccg ctcacgatca cgcgagggtc agtcccgtcg aggtttgcca
     4441 tccacacgtt gcccgcattg cgcgcggtcg gcggatccca cggagcctgg ccgtgctcga
     4501 cccagaacac gtgcgacgcg tcggccgccg ttccgagcgg gtagttctcg atgtccatga
     4561 cgagctgtgc gaagggcacg ccttgaacgt cgccgaacag tcccgacgag accgtcgccg
     4621 ttgcgatgtt gagcgtctct ttgaatggtg cgctcgcgat gcgctcggca tccacctgag
     4681 agccactcgt cgacgccgcg gcgccgacgg agaacacata cgcgtgtcct ggcttcaaga
     4741 tcggtccggc gggtggatcg ctggggtcgc agccgatact cgtcgacggt gcgttcggga
     4801 acgtgaagct cgtgttttgc gtcctgatcg tcgcgacgct tacgccatgg ctgttgttgt
     4861 cttcgtccac gtacagctcg acgacgttga tcgtgtagaa cgccggctgc aaggcactcg
     4921 caggtgggtc ccacgtgatc gttgcagcgc cgttgaggtc accgccatcg tcgaagaaat
     4981 tctggccgtt gatgcgaatg ttcctcggcg gaagcagcgg cggcaggatc ggtcccgctt
     5041 ccagcgcggc tttcgtcgtc gtccactcga tcgagtcgat gaagccaaac cggccaatgc
     5101 cgacgcgggc gttcgtgttc gggagcttgt ggaccgtgcg ctgggaggcg cgaacgtcgt
     5161 agagcacgcc ccactggcct tggaacgcgc tggtgtctgc gtacgccatc gggccgctga
     5221 cgatgtcggt ccccgattgg tcgaacatca cgagcaagtc ggcgttcgcg ccgtagaagc
     5281 catcgttcgc gctgtacggt tggccgagcg ccccgacaaa cgcgccgcag ttaacgggcg
     5341 ggcacgacac gctcggcagc acgtgatgcg gattgccgta ctgctccagc gtcgagcgcc
     5401 aggtggcgcc tcggtagtcg atgctgagtg tgttcgtcgt cggaacggac tgaagtgcga
     5461 gcgtgcgctg ctgcgtgctg ccgtcgagga cgtcgaagtc gggcgggaat tgcgcgactt
     5521 tcgtgatcgc gaggtacggc gtgttcgcag cgcccgcgtg ctgcacggtc agctgcgccg
     5581 cggctgcgcg atggccttgt gcgctgcgaa tttccgaagc cgggccggca tcgacgtccg
     5641 cgacgttgat aggaatcacc acgctgttct cgccgccact gagcgtcgtc agacgttcgg
     5701 tgttgaagtc ccagacattc gcttccgaag agaaccattc gatctggtcg ccgagctccg
     5761 tatcgctgcc ggggttccaa gggctaaggc ccgtcaacgt gagctcgagg ttcgtgtccg
     5821 tcgtcagcgg cacgaggtcc gggcgaccgg gcagcacgaa cccgaggtct ggcgctggcg
     5881 acgacgtgac gagatagtgc ttgccgtcgc ggaggatgta atcgccgttc ggcacgtgat
     5941 cgatcgagaa cgtgccgagg cccgtcgccc ggccgcacag gcgggcgtag ccgccgctgc
     6001 catcgccgac gagcgcatcg tagtcctcgc tcgcgaagtc cattgggagg tcatcgcgcg
     6061 acgtcgcgga aatgtacgtg cgcgagccac cgcccgagac ggtgcggtcg ccacttggct
     6121 ggccgacgta gccaaggctg aagtggaacg tctgcgccgt gatcgtgtgc gcgacaggat
     6181 cgatggtgcc gccgatcggc tcgtactcgg tcgtgctccc gagcttcgtc cagtacacgg
     6241 tgccggtcgt gacgcctgcg ggaagtggga gcgtgatcgt gagcggcgcc gcgaacacgg
     6301 tgccctcggg accgaactcg tataccggcg tctgcgcgcc aggggcggcg atgtccgtcg
     6361 tcgagacggt gacggtcgtc gcgctgtcga ccgcgccgta cgggacggtg acggtcaggc
     6421 cgttgtcggc ggtgaacgtg ccgccttcga cggagacagg cgcggtcacc gtgccgcagt
     6481 cctggtgctt cttgtgcgcc ttgcacttct ttgaatttgt tcgggactct tgtgtcgcgt
     6541 cctccgccgg atcgaacgca agacaagccg gtagcactgc cacgagcaac acccaagaac
     6601 gcatcggatc cttataggag gatccgtgat gcggatcgga tagcggacgg gtagctcgcg
     6661 atcaccttcg gtttaccgaa gtgcgcgctt tcccgaacct atgagatcaa acaaaacggg
     6721 ccggaatgac gccgtcgcgt cgagcatgcc gagcaccgac gcaacaccga gccgttgccg
     6781 ccagagcaac aactgcttcg ccgggagttg caagccgccc gatgcaagca cgcggccggt
     6841 cgtctcggcg aagtcgccgg cgtacttcgc gtcccagtga aacgttccgt gcgtcgcgag
     6901 cggtgcagcg agtgcgcgct cccattcgcg gtgcgcgttc gtcgcgagcg agtcggtgcg
     6961 acgcaaaagg cccgacctcg cgagtgccat gcggaagcgc tccgccgcgg aggccggatc
     7021 ctcgtcgagg aggccccacc agatttcgcg atcgctctcg cgtacctcgt cggggagcgt
     7081 cagcgcgcag ccgaagtcga ggcaatgcac gttgacgtga ccatcgtctt cgacctcgat
     7141 gaggaagttg ccgggattgg gatcggcgtt gagcaggcca tgcgcgagcg gtgagcccca
     7201 cgcgaacttg tagatggcgg cggcggcctg cgcgcgaacg tcgggggcac cgcctgccgc
     7261 gatgtcgacg acggtcttgc cgcgggcgcg cgttgtcgtc aggacgcgag cagacgacag
     7321 ctcgtcgatg acgcgcggga tgcgtaccga cttgtcgcca tcccacgcct tcgcgaatgc
     7381 ggtctgcgag gcggcctcgc gcgcgtagtc gagctcgtcg cggacggcgt cgccaagtgc
     7441 gcgcagcgcg gcgtcgtcga gcgagcggcc gatttcggcg ccggcgagct tgcggatgaa
     7501 ggattcgtcg tcgaggtcgg cgcgcagcgc gtccgcgacg cccggatact gcactttgac
     7561 gacgacgtgg gtgccgtcac gcagcgtcgc cgcgtggacc tgcccgagcg atgcggcggc
     7621 gatcggagtt gcgtcccacg acgcgaacag cgcttgcggc gccttgccga gatccttctc
     7681 gatcacgcga gagatatcgc cggccgatag cgatggcgcc ttatcccaga gcccgccgag
     7741 taccgcgcga gctgccgtgc tagcaccgcg gccgcgaccg agcgatgcgc ccgggtcata
     7801 ggcagcaagc tgcgcgacct tcgcgaggcc gcctttgagg cgacctgctt gcgcagcgag
     7861 cacacgcgcc gcttcttcgt cgacgacgtg atcgtcttcg tcagagctcg tcggatccgt
     7921 cgtgagcacc tgcttggcgc gggcgaacag gcgcgcaaag ccaaccttgc gctccgccca
     7981 ctttcgctcg tcgctcaacc tggacctcca gcgagatccg caatccgatg cgcgccagac
     8041 gggtcatcgg tccagaacag ctcgacgcgg ttcttgccgg tcttctttgc gcgatagagc
     8101 gcacggtccg cgcggcggac gagatcctcg ccagactgcg ccttgctctc cgggaacgcg
     8161 gcaatgccca gggatgcggt gaccgacagt gcgcgcggcg ggtcgtcgtc cgtcgtgatg
     8221 cggagctccg cgatcgcttc gcggatgcgc tcggccaggt tgtacgcgcc gaccatctcg
     8281 gtgcgcggaa ggatcacggc catctcttcg ccgccgtagc gcgcggccgt atcgacgccg
     8341 cgcatctgcg ccttgatgac gttcgcgatc gcacgcagca cacggtctcc gacgagatgc
     8401 ccgtacgtgt cgttgagttt cttgaaatcg tcgacgtcga gcatcacgac cgagaacggg
     8461 ctgccgtagc gacgcgagcg ttcgatctct tcttcgatgc gcgcgtcgaa gtaacggcgc
     8521 acgaacagcg ccgtaaggcc gtcgaccatc gcgagctcgt agagatgcgc gttctgcagc
     8581 gccgacgcga tctgcagcgc caacgattcg agcagtcgct ggtgctccgg cgagaatgcc
     8641 gcggcgcgcg atgactgcac cgcgacgacg ccatcgcaat cgccgtacat gaacagcggg
     8701 acgccgagcc acgaccgaat gccgtgatcc gcgtccgtcg cgatctccgc gtcttccgcg
     8761 aggtcgtcga tgcgtcgagc cgttgcgttt gcgatgaccc agccggcagc gccctcgcct
     8821 gccgccatcg tgtgacggat gaatttatcg gtgcgcttgt cgaagccgtc catgacgagg
     8881 tccgagtcgc gaccggcgcg gcgatggatg aacgtcacgg cgtctgcttc agggattgct
     8941 ttgcacgcct cgcgcgcgac cgcttcgacg agctcttcga gctgcagcgc agcggagaac
     9001 cggcggcccg tggcgttcag gatctcgaga tcgtggacgc gctcctgcag ctcgtgcctc
     9061 gcgcgcgaga gccgcgcaaa gacgaagttg atgagcagat acgtcagcga cagcagcatg
     9121 aagccgagcg gttgctcggg gtcgtagagc agaacgagca cgacgccgat cggcatcagg
     9181 gacgcctcgg acgagatgcc cggcaacgcg agctgccgca gatattgccg ccagctgtga
     9241 ccgaggagcc agag
//
LOCUS       DATLBZ010000701         4436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6591680, whole genome shotgun sequence.
ACCESSION   DATLBZ010000701 DATLBZ010000000
VERSION     DATLBZ010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6591680"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VMZ53_34160"
     CDS             <1..414
                     /locus_tag="VMZ53_34160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33614.1"
                     /translation="LDAVPAAPTVVPTTIEWDAAAIGQDALHVHASWTVDARVSAVVP
                     PGPHVVVWDAVLSPDATSATLPKLDGDLQTAIAPSSIVAGDIVLRAVDSSVLDGFGAV
                     VGSGIRAEETAQVSAIVGAPEGGQVRVAQSLGVTQ"
     gene            507..1262
                     /locus_tag="VMZ53_34165"
     CDS             507..1262
                     /locus_tag="VMZ53_34165"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HUS33615.1"
                     /translation="MLVGMVVASPVRAESKTPLRVMTYNVNYANPDFDSALDAIAKAD
                     VDVVLLQEITSEWKRALEKRFEKQYPHQMFRIHTRAAGGLAVLSKHAITAEELFASPE
                     RGWFPAERLVLDSPLGAVQILHVHLRPAIDNGSWIKGFMTTPPMRRREIESYWKKIAG
                     DLPTIVAGDFNEDPTGTAVAFLEKQGLTRATTTGPTTWHYVVWNAGKKNDLLKLDIDH
                     VMVDKRLSAHDGEVLDAGASDHRPVIVTIEPSR"
     gene            complement(1270..2499)
                     /locus_tag="VMZ53_34170"
     CDS             complement(1270..2499)
                     /locus_tag="VMZ53_34170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUS33616.1"
                     /translation="MVAPPTTPRSFAVDVARVSASFQDLARQMGAQFLDKQEIIRLMT
                     ISAIAGEHMVIVGPPGTAKSAMIDMFSKLIDAKYFEYLLTRFTEPNELFGPVDISAFR
                     EGRYTRRLDNMLPTAEIVFLDEIFKSNSAILNSLLHVINERKFQNGPEVVPVPLISLY
                     AASNEVPNDENLAAMFDRFLVRVLSDNLDSYHFHELMKKGVSLELRKMTGRGVDAQGQ
                     RTGELRPVTSARELRQIQQNFDKFMIFPEEFLTKYKGLIFQIRSEGISVSDRRAVKLL
                     KLFAASAVFDGRTRVHDGDFFILRHIWNNLDQVELLEEIINPIVDAYYREHPDERRFI
                     GPSASLDDLLTELGLIRELLTSGAELSDIQLFSQLKNLNEIKVALAALPGDAAARMIR
                     EVDQLLETVFASSKFGL"
     gene            complement(2517..3719)
                     /locus_tag="VMZ53_34175"
     CDS             complement(2517..3719)
                     /locus_tag="VMZ53_34175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33617.1"
                     /translation="MARPNVADFLTKFVKPLVAGGELHIGAPIPAADVARWEHELVNA
                     SVEQVAVDDARTNVLSTLVVRPPALVLDIDELSLAAGLHNALFLVHPRAELWSVSDRQ
                     RRKIIDTALSLVSQPLTRHRTRVMARHSLLHNLFHLKRSDVKVSWWTGHARFQGQRPS
                     KRLTAWKSVRRVREEETIVGFDELLAVPDTAPVIATLLRRTPLTQLLDSHPGSPPLHW
                     EDAVFILRDPELARAVAYRIIPDGAPRDQIAGPARLAAAFEQMLERAPDEADVRAVAA
                     FLVHMSALFCLAEINMREQNAKSPLISQVLASEAAAQRPRGLTTLFALPGALATVDAR
                     VATPPGIDQLPALAARWKVHRTQTAELLSEAVIDALAQRLRRHLRGERRDPAEVVTLS
                     PEAPPPAT"
     gene            complement(3720..>4436)
                     /locus_tag="VMZ53_34180"
     CDS             complement(3720..>4436)
                     /locus_tag="VMZ53_34180"
                     /inference="COORDINATES: protein motif:HMM:NF040100.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33618.1"
                     /translation="DTFHVFWPGDRVDVLPGPLQLSDIGDLTRVDLEMDKPRDPAQLG
                     GGAPDPIGVDLKLAAAMGPPRRVVATLIEPQHAQRLKRILFALPPTSLKGHKVAVTDR
                     GILVVGSENLDVIPLGQLLYDLGGTNNEIARGVYVPLGMDIVPRVSPDVIARALGHQG
                     GVVTVFTTDNRPFQIPDGAFQTLERRSIAKLDVDRAETIDYAADVAPEAQVVNDAVGR
                     FALWGFPDAPDKKQLPPGDK"
BASE COUNT          766 a         1361 c         1558 g          751 t
ORIGIN      
        1 ctcgatgctg tgcccgccgc gccgacggtc gtaccgacca cgatcgagtg ggatgccgca
       61 gcgatcggtc aggacgcgct gcacgtgcat gcgagctgga ccgtcgatgc cagagtgtca
      121 gcagtcgtgc cacccgggcc gcacgtcgtc gtgtgggatg ccgtgttgtc acccgatgcg
      181 acgagcgcga cactgccgaa gctcgacggc gatttgcaga ctgcgatcgc accatcgagc
      241 atcgtggcgg gcgacatcgt gctgcgcgcc gtcgatagct ctgtgctcga cggatttggg
      301 gcagttgtcg gatccggcat tcgcgctgag gaaaccgcgc aggtgagcgc catcgtcggt
      361 gcgccggagg gcgggcaagt tcgcgtcgcg caatcgctcg gcgtcacgca ataagagtcg
      421 ctccgaggtc gttgtcgacc gggatgcgtc gtcgcggatc tcggtcatac atgatgacct
      481 tgcgcctact accgtggctg ttcgtgatgc tcgtggggat ggtcgtcgcc tcgccggtgc
      541 gtgcggagtc caagacgccg ctccgagtga tgacctacaa cgtcaactac gcgaacccgg
      601 acttcgactc cgcgctcgac gcgatcgcga aggcggacgt cgacgtcgtg ctgctgcaag
      661 agatcacgag cgaatggaag cgcgcgctcg agaagcgctt cgaaaagcag tacccgcatc
      721 agatgttccg cattcacacg cgcgcggccg gtgggcttgc agtgttgtcg aagcacgcga
      781 tcacggccga ggagctgttc gcgtcgcccg agcgcggctg gtttcccgcc gagcggctcg
      841 tcctcgattc gccgctgggc gccgtgcaga tcctgcacgt gcacctgcgt cctgcgatcg
      901 acaacggcag ctggatcaaa ggcttcatga cgacgcctcc catgcgccgc cgcgagatcg
      961 agtcgtattg gaagaagatc gcgggcgact tgccgacgat cgtcgccggc gacttcaacg
     1021 aggatccgac gggcaccgcg gtcgcgttcc tcgagaagca gggcctgacc cgcgcgacca
     1081 cgaccggtcc gacgacgtgg cactacgtcg tgtggaatgc gggcaagaag aacgacctcc
     1141 tcaagctcga tatcgatcac gtcatggtcg acaagcggct gtccgcgcac gacggcgaag
     1201 tcctcgatgc gggcgcgtcg gatcaccggc cggtgatcgt cacgatcgag ccgagccgct
     1261 agagcgcgac tacagcccga acttcgacga cgcgaacacg gtctcgagca gctgatcgac
     1321 ctcgcggatc atgcgtgcgg cggcgtcgcc gggaagtgcg gcgagcgcga ctttgatctc
     1381 gttgaggttc ttcagctggc tgaacagctg gatgtccgag agctccgcgc cgctcgtgag
     1441 cagctcgcga atgaggccga gctcggtgag gagatcgtcg aggctcgcag acggaccgat
     1501 gaagcggcgc tcgtccgggt gctcgcggta gtacgcgtcg acgatcggat tgatgatctc
     1561 ttcgagcagc tcgacttgat cgaggttgtt ccagatgtgg cgcaggatga agaagtcgcc
     1621 atcgtggacg cgcgtgcggc cgtcgaagac cgcgctcgcc gcgaacagct tcagcagctt
     1681 taccgcgcgg cggtcggaga ccgagatgcc ctcgctgcgg atctggaaga tgagtccctt
     1741 gtacttggtc aggaactcct cgggaaagat catgaacttg tcgaagttct gctggatctg
     1801 ccgaagctcg cgcgcgctcg tcacgggacg aagctcgccg gtgcgttgtc cctgcgcgtc
     1861 gacgcctcgc cccgtcatct tgcggagctc gagcgagacg cccttcttca tcagctcgtg
     1921 gaagtgatag ctgtcgaggt tgtcggacag cacgcgcacg aggaagcgat cgaacatcgc
     1981 ggcgaggttc tcgtcgttcg gcacttcgtt cgacgccgcg tagagcgaga tgagcggaac
     2041 cggcacgact tcggggccgt tctggaactt gcgctcgttg atgacgtgga gcagcgagtt
     2101 caagatcgcc gagttcgact tgaagatttc gtcgaggaac acgatctcgg cggtcggcag
     2161 catgttgtcg aggcgacgcg tgtagcggcc ctcgcggaat gccgagatgt cgaccggacc
     2221 gaacagctcg ttcggctccg tgaagcgcgt gaggaggtac tcgaagtact tcgcgtcgat
     2281 cagcttcgag aacatgtcga tcatcgccga cttcgcagta ccgggcggac cgacgatgac
     2341 catgtgctcg cccgcgatcg cggagatggt catcaggcga atgatttcct gcttatcgag
     2401 gaactgcgcc cccatctggc gcgcgaggtc ctggaacgac gcactgacgc gcgcgacatc
     2461 gacagcaaag ctgcgcggag tggtgggcgg agcgaccatc tgttgtcgac cttacatcaa
     2521 gttgcgggcg gcggggcctc gggagacagg gtcacgacct ccgctggatc gcggcgttct
     2581 ccccggagat ggcggcgtag gcgttgggca agcgcgtcga tcacagcctc gctgagcagc
     2641 tccgcggtct gcgtgcgatg gactttccag cgggcggcga gcgcgggcag ctggtcgatt
     2701 ccaggcggcg tggcaacgcg agcgtcgacg gtcgcgagcg caccggggag cgcgaacagc
     2761 gtggtcagcc cgcgtggccg ttgcgccgct gcctcggacg cgagcacctg cgagatgagc
     2821 gggctcttcg cgttctgctc gcgcatgttg atctcggcga ggcagaacag cgccgacata
     2881 tgcacgagga acgcggcgac cgcgcggacg tcggcctcgt cgggagcgcg ctcgagcatc
     2941 tgctcgaagg cagcagcgag gcgcgcggga ccggcaatct gatcgcgcgg agcaccatcg
     3001 gggatgatcc ggtacgcgac ggcgcgcgcg agctcggggt cgcgcaggat gaagaccgcg
     3061 tcttcccagt gcagcggcgg cgagccgggg tgactgtcga gcagctgcgt gagcggcgtg
     3121 cggcgcaaca gcgtcgcgat gacaggcgcg gtgtctggca ccgcgagcag ctcgtcgaag
     3181 ccgacgatcg tttcttcctc gcggacgcgg cgcacgctct tccacgcggt gaggcgcttg
     3241 ctcgggcgct gaccttgaaa gcgcgcgtgg cccgtccacc acgagacttt gacgtcgctg
     3301 cgcttgagat gaaacaggtt gtgcagcagc gagtgccgcg ccatcacgcg cgtgcggtga
     3361 cgcgtgagcg gttggctcac gagcgagagt gcggtgtcga tgatcttgcg gcgctggcga
     3421 tcgctcacgg accacagctc cgcgcgcgga tgaacgagaa acagcgcgtt gtgcaggcct
     3481 gctgcgagcg agagctcgtc gatatcgagc acgagcgcgg gcgggcgaac gacgagcgtc
     3541 gacaggacat tcgtgcgcgc atcgtcgact gcgacttgct cgacgctcgc gttgacgagc
     3601 tcgtgctccc agcgcgcgac gtcggccgcg ggaatcggtg caccgatgtg cagctcgcca
     3661 cccgcgacga gtggcttgac gaacttcgtc agaaagtctg cgacgtttgg ccgagccatc
     3721 tacttatcgc caggcgggag ctgcttcttg tcgggcgcat cggggaagcc ccacagcgcg
     3781 aagcggccga cggcgtcgtt gacgacctgc gcttcgggcg cgacgtccgc ggcgtaatcg
     3841 atcgtctccg cgcgatcgac atcgagcttt gcgatcgagc ggcgctcgag tgtctggaaa
     3901 gcgccgtcgg ggatctggaa cgggcgattg tccgtcgtga acaccgtgac gacgccgcct
     3961 tgatgaccga gcgcgcgcgc gatcacgtcc ggcgagacgc gaggcacgat gtccatgccg
     4021 agcggaacgt agacgccgcg cgcgatctcg ttgttcgtgc cgccgagatc gtagaggagc
     4081 tgaccaagtg ggatgacgtc gaggttctcg gagccgacga cgaggattcc gcgatcggtg
     4141 accgcgacct tgtggccctt gagcgacgtc ggaggaagcg cgaacaggat gcgcttgaga
     4201 cgctgcgcgt gctgcggctc gatcagcgtc gcgacgacac gcctcggcgg gcccatcgcg
     4261 gctgcgagct tgaggtcgac gccgatcgga tcgggcgcgc cgccgccgag ctgcgccgga
     4321 tcgcgaggct tgtccatctc gagatccacg cgcgtcagat cgccgatgtc cgaaagctgg
     4381 agcggaccgg gcaacacgtc gacgcgatcg ccgggccaga acacgtgaaa cgtgtc
//
LOCUS       DATLBZ010000702         8898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_8184064, whole genome shotgun sequence.
ACCESSION   DATLBZ010000702 DATLBZ010000000
VERSION     DATLBZ010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8184064"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1001)
                     /locus_tag="VMZ53_34185"
     CDS             complement(75..1001)
                     /locus_tag="VMZ53_34185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33619.1"
                     /translation="MTTKRSGRYSDQPPVIAAGPKAAPLPLVTQPIVPLDDSMQTRVD
                     GRIDPALTIPSKNDGAVLFGKANLVATTVASVPLSQKPMPAGPPPEIGLTQHTLPSDE
                     TPDGRLVLMREPDSERSAAYRVLRHHLLEAGRPQVVIVSSAQQGEGKTTTALNLALAL
                     AECGRAKVLLAETHVRRPQLANVFRCVPPWCFAEQLAAHRHQPMLPWSLVDIPQTWLH
                     LAPINPRIEKTQLLDAPAFAIAMERLRLAGYDHIVIDAPPVLGSADVNLMADAADAVL
                     FSVRAKTSTTRDVRKAVDQIGQKKVIGTVLCE"
     gene            complement(998..2560)
                     /locus_tag="VMZ53_34190"
     CDS             complement(998..2560)
                     /locus_tag="VMZ53_34190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33620.1"
                     /translation="MMTPRDKLQRLVDLGRKTWRYAWLLAIFAAAGIVLSVAFALLKP
                     KKFQSYAVLFYQERIQTSLLSNREEQVQRNIGDRYRELLLARGQLAQIVQDKKLNPFP
                     DEPDEDVAIDKLRQSVKFEPRGANAFRITYTDSEAERAKLVTDKLTQMLQERDEALRN
                     EQAKATVTFALKQKDDAQIELSKMELELAQFLAKHPEFAQDATQPVAAGAVAGEGASI
                     RQFGNNQNKKPVNTGNARLYALERQRQRIQARLDAPPDAPPIRIPSPSTPEKIAAEAE
                     VAQARNELASANREMTEAMAQYTEQHPSVIRAKDRVAAAKAKLARAQTAVPPDVETAV
                     APATEADRDKLRQELKTLEDQIASEQKRGEGKAATAAAATDTTTNWVVQLETEHAKLR
                     RDVTEGRERVEALADSVFRAQMDANQKLAETGGRLSVVDPAFKPVKPSGPGKTVIVIA
                     GLALFLALGAGLALGLAVIDDRLYRRSDLEELGLAVLAVIPPSSHSAAGAKPKAKPAA
                     NAKRTKRETRAS"
     gene            complement(2557..3669)
                     /locus_tag="VMZ53_34195"
     CDS             complement(2557..3669)
                     /locus_tag="VMZ53_34195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33621.1"
                     /translation="MSTRLPNDEWYESEESTRKGMIAEMQRIKRRTRVRPIPVIVLAL
                     LITGAITYKMATKKGHYQAEVVLAIREGSLLSEDKRTGLPVGELKEFVTGVLMPDAKL
                     AELIEQRNLHRLRKRMGMPWAINELREQYEIEVWRNSFIYYDPENPNREASARIGLTV
                     TDEDPDSAYLLAHDLAQIIKDSVRDHRQDVANKVAAQIAHVREGTQQRITELELAKSE
                     KLVQIANAKKEGKQALAQALQMQLLKIDHQIGDGEKSLAEIAASPEALADRISEAGLD
                     LIVEIVAERRPDRPESKGLLIAMIAVVVGVGALLGSALVLGSFDSRVHDTDDVERLGL
                     PVLGHVPGFPGDTVGSLEARGVQRARVPSFLRWRSQ"
     gene            complement(3672..4847)
                     /locus_tag="VMZ53_34200"
     CDS             complement(3672..4847)
                     /locus_tag="VMZ53_34200"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUS33622.1"
                     /translation="MRVLAITKIFPNAAEPLSAPFNRQQFAALRELCDLEVMATIPWF
                     PGAGLLSKWSSAGKLASVPKRETIAGIEVTHPRTLFLPKLAHATWGPLYAASIAPILA
                     PYRKKVDVVLGSWAYPDGFAAIVAAQLLGVPAVVKLHGSDINLIAKIPGPRRMMAWAL
                     PRAARVVAVSKPLAAEVVALGVPEDRVKVVMNGVDADLFKPGDRKAARAQLDLPQDKP
                     LAIYVGNLKPEKGVVDLGKAWAGVVKQVPDATLLVIGDGPLKGELEGVTQSLGERVRL
                     IPRQPLERIPLYMAAADILVLPSHFEGTPNVVLEALASGRRVVATSVGGVPDLITDAT
                     LGTLVPAGDPGALGNAIATALRSPYDAEEVARKGARGGWAASASALHAVLQEAAKVA"
     gene            complement(4877..5182)
                     /locus_tag="VMZ53_34205"
     CDS             complement(4877..5182)
                     /locus_tag="VMZ53_34205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HUS33623.1"
                     /translation="MPSTFDVQRSDKATTLAVRGSLDINTAPQLGDEVDRIINEKPKT
                     VIVDLSGLDLIDSSGVAALVKLYKGVRSGGGAITISGARDQPLAIFKLLRMDKVFNL"
     gene            complement(5166..5645)
                     /locus_tag="VMZ53_34210"
     CDS             complement(5166..5645)
                     /locus_tag="VMZ53_34210"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33624.1"
                     /translation="MAPIRLTVPGTLRYRDVAVRIVAEAARLVSSSSQRDPKNVLDHD
                     VRDPFDTAVVSAFMEIFNNVAIHAYQRKGGGTIEIAITPTDGELVIEVKDHGSPFDID
                     EVAPLPSDLDPECLPEGGMGIHIAKTMLDECSYEPGPPNLWRLMKRLPQGTSNAVNV"
     gene            5694..6428
                     /locus_tag="VMZ53_34215"
     CDS             5694..6428
                     /locus_tag="VMZ53_34215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HUS33625.1"
                     /translation="MRRSVRFAKRAIDVMGGALGLIVSAPLMPFIAAAIYMESPGPVF
                     FRQRRAGSLKKITNDNGIKKLQFEEFQMHKFRTMIPNAEAKTGVVVAGADDPRITKVG
                     KFLRKSRLDELPQFWDVLRGKMSLVGPRPERPELLQDLSYAIPLFEERMRDVKPGITG
                     LAQISLGYTGSIPEGSEIGKLASTLQNPWDLEETKGSLADDMRIKLLYDLAYTASLER
                     FDTFLRTELGIIFKTPLVMLKMTQGR"
     gene            6535..7161
                     /locus_tag="VMZ53_34220"
     CDS             6535..7161
                     /locus_tag="VMZ53_34220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33626.1"
                     /translation="MNARIVVVLLVSLAGCYRTKNLAAPPTPSPQLPAVPLSGAPGPG
                     LTRVVLDVEDGPAVVEELHGGSLSGATGSHVFGGSLQVAQRVCVTPCVFDTKPGGHEL
                     RFTLADDSTRTSVGFVNADQRVSAYRHALGRDRSSAWKGFVGWPLLVAGALLDVGMVS
                     ALSDGREMTGGSIAGAAFSVGITALGGWLVYGSVVEKQPGSGTQWYPE"
     gene            7181..8104
                     /locus_tag="VMZ53_34225"
     CDS             7181..8104
                     /locus_tag="VMZ53_34225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33627.1"
                     /translation="MGVVPISIEYVAPPACPDEISVRAGVIARLGTDPFQGDAPRVAM
                     IDVQPTGAGFVAAIELREPGRPASRRAVGPALRCEDAVDALELALAVIIDPTYSAQPP
                     APPPPHPLPQPAPVAAVYEPPGWRVPTPASPSIRRHELALAIGVVAGTHPEAEGTFGL
                     RAGYMVDEHWRIGMVGRLANGNGTLTMDAAGNYRHYETRTSELEVEGCRRYRFLLACA
                     LVGVGTKSVHIDRYDYALGVSQEVADYSDPFAIAGANLSLEVPLGRAYLRPLVEGSAP
                     LPNVPIESEGLRHLELPVVRFAFDLSLGYRW"
     gene            8101..8610
                     /locus_tag="VMZ53_34230"
     CDS             8101..8610
                     /locus_tag="VMZ53_34230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HUS33628.1"
                     /translation="MSAAALDFAAVYRDNLDYVWQSLRRLGAPPSDLEDLAHEVFLVV
                     HRKLVDYDTTRPIKPWLFGIAFRVASDHRKKLARRGDHGEDIADVRAASDASPEAQTM
                     RRQAIDLARRALESVDDEARAVFILAELDGVAVTDVATSLEIPVNTAYTRLRRARLAV
                     ANVLTGATP"
     gene            8607..>8898
                     /locus_tag="VMZ53_34235"
     CDS             8607..>8898
                     /locus_tag="VMZ53_34235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33629.1"
                     /translation="MSEPDDKPLPPWQRAALLSEGLEGGPSQEQRARMAEKLALAVGI
                     SAAALLPGGGGSGGGGSGGGNGNGNGNGNGNGSGNGSGNGSGSGNGNGNGNGS"
BASE COUNT         1429 a         2849 c         2983 g         1637 t
ORIGIN      
        1 tccgcgtccg cttccgcttc cgcttccgct tccgcttccg cttccgcttc cgcttccgct
       61 taccgtgcgg cggcctactc gcagaggacg gtgccgatga ctttcttctg gccgatctga
      121 tcgacagcct tccgaacgtc acgcgtcgtc gatgtcttcg cgcggaccga gaagaggacc
      181 gcgtcggccg catccgccat gaggttcacg tcggcggagc cgagcaccgg cggcgcatcg
      241 atcacgatgt gatcgtagcc agctaggcgc agccgttcca tcgcgatcgc gaacgcgggc
      301 gcgtcgagca gctgcgtctt ctcgatgcgc gggttgatcg gcgcgaggtg cagccacgtt
      361 tgcgggatgt cgacgagcga ccacggcagc atcggctggt ggcggtgcgc ggcgagctgc
      421 tcggcgaagc accacggcgg cacgcagcgg aagacgttcg ccagctgcgg gcggcgcacg
      481 tgcgtttcgg cgagcaggac tttcgcgcgg ccgcattcgg cgagcgcgag ggcgaggttg
      541 agggcggtgg ttgtcttgcc ttcgccttgt tgggcggacg agacgatcac gacttgtggg
      601 cgacctgctt cgagaaggtg gtggcgcagg actcggtatg cggccgagcg ttcgctatcg
      661 ggctcgcgca tcagcacgag gcggccgtcg ggcgtttcgt cggacggcag cgtgtgctgc
      721 gtcagaccga tttccggggg cgggcctgca ggcatgggct tttgcgagag cggcaccgac
      781 gcgacggtgg tcgcgactag gttcgctttg ccgaacagta ccgcgccgtc gttcttcgag
      841 gggatcgtca acgcgggatc gatgcgacca tcgacgcgcg tctgcatcga atcgtcgagc
      901 gggacgatcg gctgcgtgac gagcggcagc ggcgcggcct tgggacctgc ggcgatcacg
      961 ggcggttggt cggagtagcg tcctgagcgc ttggtggtca cgaggccctc gtttcacgct
     1021 tcgtacgttt cgcgttggcg gctggcttgg cctttggctt ggcgccggcg gcggagtgcg
     1081 acgacggcgg gatgaccgcg aggacggcga ggccgagctc ttcgaggtcg ctgcggcgat
     1141 agagccgatc gtcgatgacg gcgaggccga gcgcgaggcc cgcgccgagc gcgaggaaga
     1201 gggcgaggcc cgcgatcacg atgacggtct tgccggggcc cgacggcttg acgggcttga
     1261 acgcggggtc gacgaccgag aggcggccgc cggtttcggc gagcttttgg tttgcgtcca
     1321 tctgcgcgcg gaacacggag tcggcgagag cttcgacgcg ctcgcggcct tcggtgacgt
     1381 cgcggcggag cttggcgtgc tcggtctcga gctggacgac ccagttcgtc gtcgtatcgg
     1441 tggcagcggc ggcagtcgcg gccttgccct cgccacgctt ctgttcggac gcgatttgat
     1501 cttcgagcgt cttgagctct tggcggagct tgtcgcgatc ggcttcggtg gccggcgcga
     1561 cggcggtctc gacgtcgggc gggactgcgg tttgggcgcg cgcgagcttg gccttggcgg
     1621 ctgcgacgcg atccttcgcg cggatcacgg acgggtgctg ctccgtgtac tgcgccatcg
     1681 cttcggtcat ctcgcggttt gcggaggcga gctcgttgcg tgcttgcgcg acttcggctt
     1741 cggccgcgat cttctcgggc gtcgacggag acgggatgcg gatcggcggt gcgtcgggcg
     1801 gcgcgtcgag gcgcgcttgg attcgctggc gctgacgctc gagagcgtag aggcgagcgt
     1861 tgcccgtgtt gaccggcttc ttgttctggt tgttgccgaa ctgacggatc gatgcgcctt
     1921 cgccggcgac cgcgccggct gcgaccggct gcgtggcgtc ttgtgcgaac tcgggatgct
     1981 tcgcgaggaa ctgtgcgagc tcgagctcca tcttggacag ctcgatctgt gcgtcgtcct
     2041 tttgcttcag cgcgaacgtg acggtggcct tcgcctgctc gttgcgaagc gcttcgtccc
     2101 tctcctgcag catctgcgtg agcttgtcgg tgacgagctt cgcgcgctcg gcttcgctgt
     2161 ccgtgtacgt gatgcggaac gcgttcgcgc cgcgcggttc gaacttcacc gactgacgga
     2221 gcttgtcgat cgcgacatcc tcgtcgggct cgtcggggaa cgggttcagc ttcttgtcct
     2281 ggacgatctg cgcgagctga ccgcgcgcga gcaggagctc gcgatagcga tcgccgatgt
     2341 tgcgctgcac ttgctcttcg cgattggaga gcaggctcgt ctggatgcgc tcttgataga
     2401 acagcaccgc gtacgactgg aacttcttcg gcttgaggag cgcgaacgca accgacagca
     2461 cgatgccagc cgccgcgaag atcgcgagca gccatgcgta gcgccaggtt ttgcgtccga
     2521 gatcgacgag acgctgcaac ttgtctctgg gagtcatcat tgcgaccgcc atctcaaaaa
     2581 cgatggtaca cgagcacgtt gcacaccacg tgcctcgagg gaaccgaccg tgtcacctgg
     2641 gaatccagga acatggccaa gaacgggaag accgagtcgt tcgacatcat cggtatcgtg
     2701 cacgcgggaa tcaaatgaac cgagcacgag tgctgatcca agtaacgcgc caactcccac
     2761 gacgaccgca atcatcgcga tgaggaggcc cttgctctct ggtcggtccg gccggcgctc
     2821 tgccacgatc tcgacgatga gatcgagccc cgcttcagag atgcgatcgg cgagtgcttc
     2881 gggacttgcc gcgatctcgg cgagcgactt ctctccatcg ccgatctgat ggtcgatctt
     2941 caagagctgc atctgcagcg cttgtgcgag cgcctgcttg ccttccttct tcgcgttcgc
     3001 gatctggacc agcttctcgc tcttcgcgag ctcgagctcg gtgatgcgtt gctgcgtgcc
     3061 ctcgcgcacg tgtgcgatct gcgcagccac tttgttcgcg acgtcctggc ggtgatcgcg
     3121 aaccgagtcc ttgatgatct gtgcaaggtc gtgcgcgagg aggtacgcgc tatccggatc
     3181 ctcgtcggtg acggtgaggc cgatgcgagc gctagcctcg cggttcggat tctccgggtc
     3241 gtaatagata aacgagttgc gccagacttc gatctcgtat tgctcgcgca gctcgttgat
     3301 cgcccacggc atgcccatgc gcttgcgcag gcgatgcagg ttgcgctgct cgatcagctc
     3361 cgcgagcttc gcgtcgggca tcagcacgcc ggtgacgaac tctttgagct cgccgacggg
     3421 caagcccgtg cgcttgtctt ccgacagcag cgagccttcg cgaatcgcga ggacgacttc
     3481 ggcttggtag tggcccttct tcgtcgccat cttgtacgtg atggcgccgg tgatgagcag
     3541 cgcgagcacg atgactggaa tcggccgaac acgcgtgcgg cgcttgatgc gctgcatctc
     3601 cgcgatcatg cctttacgag tcgattcttc cgactcgtac cactcgtcgt ttggcagacg
     3661 cgtggacatg gtcaggcgac cttagcagcc tcttgcagca ctgcatgcaa tgcagacgcg
     3721 cttgcagccc atccaccgcg agcacctttg cgcgcgacct cttcggcatc gtaaggcgac
     3781 cgcaaggcag tagcgatcgc attgccaagc gcacccggat cgccggcggg aacgagagtc
     3841 ccgagcgtcg cgtcggtgat gagatcggga acgccgccga ccgatgtcgc gacgacgcgc
     3901 cggccacttg cgagtgcttc gagcacgacg ttcggcgtcc cctcgaaatg cgagggcaac
     3961 acgaggatgt ccgcggccgc catgtagaga gggatgcgct cgagtggctg gcgagggatg
     4021 aggcgaaccc gctcgccgag cgattgcgtc acgccctcga gctcaccctt gagtgggccg
     4081 tcaccgatca cgagcagcgt tgcgtcgggc acctgcttga cgactcccgc ccacgccttg
     4141 ccgaggtcga cgacgccctt ctcgggcttc aagttcccga catagatcgc gagcggcttg
     4201 tcctgcggca ggtcgagctg ggcgcgcgcg gccttgcgat cgccgggctt gaatagatcc
     4261 gcgtcgacgc cgttcatcac gaccttcacg cgatcctcgg gtacgccaag cgccacgacc
     4321 tccgctgcga gtggcttact caccgcgacg acgcgcgcag cgcgaggaag tgcccacgcc
     4381 atcatccgac gaggacctgg aatcttcgcg atgagattga tgtcgctgcc gtgcagcttc
     4441 acgacggcag gaacgccgag gagctgcgca gcgacgatgg ccgcgaatcc atcggggtac
     4501 gcccacgagc cgagcacgac gtcgaccttc ttgcgatacg gcgccagaat cggcgcgatc
     4561 gacgcggcgt agagagggcc ccacgttgcg tgcgcgagct tcgggagaaa cagcgtgcgc
     4621 ggatgcgtga cctcgatgcc ggcgatcgtc tcgcgcttcg gcaccgacgc gagctttcct
     4681 gcgctcgacc acttcgagag caaacccgct cccgggaacc acgggatcgt ggccatcacc
     4741 tcgaggtcgc acagctcgcg aagcgcggcg aactgctggc gattgaacgg cgcggacagc
     4801 ggctctgcgg cgttcggaaa gatcttcgtg atggcgagaa cgcgcatcgt cacccgccgc
     4861 cctgcacgta ccgtgcttag aggttgaaga ccttgtccat gcgcagcagc ttgaagatcg
     4921 cgagcggctg atcgcgggcg ccgctgatcg tgatcgcacc gccgccactg cgtacgcctt
     4981 tgtagagctt cacgagcgca gcgacacccg agctgtcgat gagatcgaga ccgctgaggt
     5041 cgacgatcac ggtcttcggc ttctcgttga tgattcgatc gacttcgtcg ccaagttgtg
     5101 gcgccgtatt gatatcgagc gagccacgga cggcgagcgt ggtcgcctta tcagaacgct
     5161 ggacatcaaa cgttgacggc attggaggtt ccttggggca ggcgtttcat caggcgccag
     5221 agattcggcg gtcctggttc gtaactgcat tcgtcgagca tcgtctttgc gatgtggatg
     5281 cccatcccgc cttccggcag gcactcgggg tcgagatcac tcggtagcgg cgcgacctcg
     5341 tcgatatcga acgggctgcc gtgatccttc acttcgatca cgagctcgcc atcggtcggc
     5401 gtgatcgcaa tttcgatcgt gccgccgcct ttgcgttgat aggcgtgaat cgcgacgttg
     5461 ttgaaaatct ccatgaacgc cgacacgacg gctgtgtcga acggatcgcg cacgtcgtga
     5521 tcgaggacgt tcttcggatc gcgttgcgac gagctcgaca cgagacgcgc agcttcggcg
     5581 acgatgcgca cggcgacgtc gcgatagcgc aacgttccgg ggaccgtcag acgaatgggc
     5641 gccatcagtg gaggcaccat atcaggctca tgacgtgcat ggtacggtgc cccatgcgac
     5701 gcagtgtccg atttgcaaaa cgagccatcg acgtcatggg tggtgcgctc gggctcatcg
     5761 tgtcagctcc gctcatgccg ttcatcgcgg cagcgatcta catggagtcc ccgggccccg
     5821 tgttcttccg ccagcgtcgc gccggcagcc tgaagaagat cacgaacgac aacggcatca
     5881 agaagctcca gttcgaggag tttcagatgc acaagttccg cacgatgatc ccgaacgccg
     5941 aggcgaaaac cggcgtcgtc gttgcgggag ctgacgatcc gcgcatcacg aaggtcggca
     6001 agttcctccg caagtcgcgg ctcgacgagc tgccgcaatt ctgggacgtg ctgcgcggca
     6061 agatgagcct cgtcggtccg cgtcccgagc gtccggagct gctgcaagac ctctcgtacg
     6121 ccatcccgct attcgaagag cgcatgcgtg acgtgaagcc gggcatcacc ggccttgcgc
     6181 agatctcact cggctatacc ggctcgatcc cggaaggcag cgagatcgga aagctcgcat
     6241 cgacgctgca gaacccgtgg gacctcgaag agacgaaggg ctcgctcgca gacgacatgc
     6301 gcatcaagct gctgtacgac ctcgcgtaca ccgcgtcgct cgagcggttc gacacgttcc
     6361 ttcgcaccga gctaggcatc atcttcaaga cgccgctcgt gatgctgaag atgacgcaag
     6421 gccgctgagc ccccgcttgg tgtttggcgc cggcctgcgt ttccgtttcg ggaaaaaata
     6481 aaaatcgtag gtgcccgaaa cggacggcgg ttttgccgcc aatccccggg caccatgaac
     6541 gctcgcatcg tcgtcgtcct tctcgtttca cttgcaggct gctaccgcac gaagaacctt
     6601 gcggcgccgc ccactccctc gccgcagctg cccgccgtgc cgctgagcgg tgctcccgga
     6661 ccgggcctga cgcgtgtcgt gctcgatgtc gaggacggac cggccgtcgt cgaagagctg
     6721 catggcggct cactgagcgg tgcgactggt tcacacgtgt tcggtggctc gctccaggtc
     6781 gcgcaacgcg tgtgcgtgac gccatgcgtg ttcgatacaa agcccggcgg tcacgagctc
     6841 cgcttcaccc tcgccgacga cagcacgcgc acgagcgtcg gcttcgtcaa tgcagatcaa
     6901 cgcgtctctg catatcgcca cgcactcggc cgcgatcgca gtagcgcgtg gaagggcttc
     6961 gtcgggtggc cgctgctcgt cgcaggcgcg ctgctcgacg tcgggatggt gtccgcgctc
     7021 tccgacggtc gcgagatgac cggcgggtcg atcgcaggag ccgcgttttc cgtcggcatc
     7081 accgcgctcg gcggctggct cgtgtacggc tccgtcgtcg agaaacagcc cggcagcggc
     7141 acgcagtggt accccgagtg actgtgttac gactccgtga gtgggtgtcg ttccgatctc
     7201 gatcgagtat gtcgcgccgc cggcgtgccc cgacgagatc agcgtgcgcg ccggcgtgat
     7261 cgcgaggctc ggcaccgatc cgttccaagg cgacgcgccg cgtgtcgcaa tgatcgatgt
     7321 ccagccgacg ggtgcggggt tcgtcgcggc gatcgagctg cgggagccgg gccgccccgc
     7381 atcgcgacgc gcggtcggtc ccgctcttcg ttgcgaggac gccgtcgatg cgctcgagct
     7441 cgcactcgcg gtgatcatcg atccgacgta ctcggcccag ccgccggccc caccaccgcc
     7501 gcacccgctg ccgcagccgg cgccggtcgc agccgtctac gagccgccgg gatggcgcgt
     7561 tccgacgccc gcatcgccga gcattcgtcg ccacgagctc gcgctcgcga tcggcgtcgt
     7621 cgccggtacg caccccgaag cggaaggcac gttcggcctg cgcgccggct acatggtcga
     7681 cgagcattgg cgcatcggca tggtgggccg actcgcgaac ggcaacggca cgctgacgat
     7741 ggatgccgcc ggcaactatc gccattacga aacccggacg tcggagctcg aggtcgaagg
     7801 ctgccggcgc taccgcttcc tgctcgcgtg cgcgctcgtc ggtgtgggca cgaagtcggt
     7861 gcacatcgat cgctacgact acgcgctcgg cgtcagccaa gaagtcgctg actacagcga
     7921 tccgttcgcg atcgcaggcg cgaatctctc gctggaagtg ccgctcggcc gcgcatattt
     7981 gcgtccgctc gtcgagggca gcgctccgtt gccgaatgtg ccgatcgaat ccgagggtct
     8041 tcgccactta gagctaccgg tcgtccgttt cgcgttcgat ctgtcgcttg gatatcgctg
     8101 gtgagcgcgg ctgcgctcga cttcgccgcc gtctaccgag acaacctcga ctacgtctgg
     8161 caatcgctgc gtcgtctcgg cgcaccgccg tctgatctcg aagacctcgc gcacgaagta
     8221 ttcctcgtgg tccatcgcaa gctcgtcgac tacgacacca cacgcccgat caagccatgg
     8281 ctgttcggca tcgcattccg tgtcgcgagc gatcatcgca agaagctcgc gcgacgaggc
     8341 gatcacggcg aggacatcgc ggacgtgcgc gccgcgagcg acgcatctcc cgaagcgcag
     8401 acgatgcgac gccaggcgat cgatctcgca cgtcgagccc tcgagtcggt cgacgatgaa
     8461 gcgcgcgccg tgttcattct cgccgagctc gacggcgttg ccgtcacgga cgtcgcaacg
     8521 agcctcgaga ttcccgtcaa caccgcgtac acgcgactgc gtcgagctcg cctcgccgtc
     8581 gcgaacgtgc ttaccggagc aacgccatga gcgagcccga cgataagccg ctgccgccgt
     8641 ggcaacgcgc cgcgctgttg agcgagggcc tggaaggcgg gccatcgcag gaacaacgcg
     8701 cgcggatggc ggagaagctc gcgctcgcgg tggggatctc cgcggccgcg ctgttgccgg
     8761 gcggtggcgg ctccggcggt ggcggctccg gcggtggcaa cggtaacggc aacggcaacg
     8821 gcaacggcaa cggtagcggt aacggcagcg gcaacggtag cggcagcggc aacggcaacg
     8881 gcaacggcaa cggtagcg
//
LOCUS       DATLBZ010000703         8681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6597134, whole genome shotgun sequence.
ACCESSION   DATLBZ010000703 DATLBZ010000000
VERSION     DATLBZ010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6597134"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VMZ53_34240"
     CDS             complement(<1..623)
                     /locus_tag="VMZ53_34240"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HUS33630.1"
                     /translation="MSTSLLRGSVKELRFQRFALRVLDGLDKGKEHVCTSAAEVSVGT
                     AESNALVLSDPTVSRYHFVLQVDPQGVQLRDLGSTNGTTLGGFRVGSAYLKSGAVLTV
                     GMTRIRYDEMSEQLDEPLSDDEFFGRALGRSVAMRRLFSVLPRIAATDSTVLIEGETG
                     TGKGVLAEAIHTASSRAQKPLVVVDCGSIPPSLIESELFGHEKGAFTG"
     gene            640..1806
                     /locus_tag="VMZ53_34245"
     CDS             640..1806
                     /locus_tag="VMZ53_34245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33631.1"
                     /translation="MVPSMVAPERYQLLGPLGTGGMAEVLLAKTRGPNGFERLLAVKR
                     ILPERAKDPDAVRMLVDEARNAAGLSHHRIVQVLDIELRDGVVLYAMEYLHGQTLEAL
                     LGKARRLPLDASLAICIAVADGLHHAHERSSPIIHRDVAPSNVMVLYDGNIKLIDFGI
                     AKAANNLSNTVFGTFKGRLGYSSPEQVRCEAVDRRTDVYSLAVMLYELTTGRPAFSAH
                     DEPHMLDLMEKARITPPRELDAQYPAELEAIVMKGLARDRNARYATAAAMQLDLEAFA
                     RRADLSLTDLTISRLMAKLFATELEPWHRARNGGMTLEDHVIQATLRTLPPSTHDSIE
                     ERATQPRVARRRRRRRNRMVIGIAVVVALFAAAYLFTRWVLVDPPSLKAASPSG"
     gene            complement(1781..2509)
                     /locus_tag="VMZ53_34250"
     CDS             complement(1781..2509)
                     /locus_tag="VMZ53_34250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33632.1"
                     /translation="MRAVVCALAITACSFPEKELIDAQGTPFGCLNAPAPTKADNPAV
                     ISGTVVVAGPGTPLQNASVAGQLAGTPTSIFLAHTDASGMFRQSQNTGGTPLDLYLAV
                     SANGLFPTNYYPAYKVTHSISFPAVGAIQLFSQTDADRLAMAAGITLDPAKGHIILTV
                     DDCNGKALAGGTVTTSPAGTIRYFAQQVPSQTATSTDTLGVVLVANLPVGNVMLSATV
                     GGMNLRTHNINIVAGTFIQTEMQP"
     gene            complement(2509..3486)
                     /locus_tag="VMZ53_34255"
     CDS             complement(2509..3486)
                     /locus_tag="VMZ53_34255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33633.1"
                     /translation="MRIASSCVVAALAMSIAVPTNASAQTAEAEVLFREGQRLMKLEQ
                     YDEACEKFEASDRIEPASGTELNLAKCRELNGQTASAWAMYLKAAASAKKTNNDKRAA
                     EAKTRANALADQLVYLTIEVPPEVRVEGLVIKRNKVPLDPGLWDTKVPVDPDEYRITA
                     QAPGYKRWSETVTVKTKNKVIEVPTLEKREGPDEQEEPIEERPRKLPRVPNALEHKYQ
                     TEATTLALVGVGGLVVGTAFALYGRNLEQQSDQLCPSIKCADANGVALNRTARTDALV
                     ANVSWAIGGAALITAGVLWYVGKPASPDSVAIVPVVDDTTAGFAVGGHF"
     gene            complement(3470..4768)
                     /locus_tag="VMZ53_34260"
     CDS             complement(3470..4768)
                     /locus_tag="VMZ53_34260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33634.1"
                     /translation="MNFGPYRVVRKLGQGGMGAVFLGEHTLIGRRAAIKVLHRERSTQ
                     RESVERFFTEARATSAMEDPGIVQVYDFGVTTDGTAYLIMEFLDGEPLSARLRNRGAL
                     HPSDAVRITRQIAGSLAAAHAMGIVHRDLKPENLFMVRDKEALGGERPKILDFGIAKL
                     GDEELTQRFQTRTGAVMGTPAYMSPEQCNDSGRIDHRTDVYSLGCVMFHLLTGRPPFD
                     LEGVGAIIAAHLREPAPVPSRVAPRVPELLDPIVARCLAKNPDDRYSSMTELQEACEA
                     AFAQLPAAPPISSSEGVALREVREDAITTLGTSAGESLTDARPIPLRLWIGIATIAIA
                     AGIVLAVTTHGGSSEKVVPAVQPRVEIEPTPPPVVAPPAPIVPQTVPVETGASGSVQG
                     SDVEVGEPATTAVPSKTPVKRPGRKTKKGHTNAETYEDRF"
     gene            complement(4781..5782)
                     /locus_tag="VMZ53_34265"
     CDS             complement(4781..5782)
                     /locus_tag="VMZ53_34265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33635.1"
                     /translation="MRIVLATALVASLAACAEDANEVSEHDGFDDTALDGKADESQAS
                     RRVVHTFSDKKLFPEGGAYDSVDRAFYVGSLQHGTVTRVDADGRESLFYAGTGEDKRY
                     TLGMQVDAQRRALWVCTTKDSLGTIWIFDLESGARTASIDLTQVNPEAACNDVLLDGD
                     TALVSDRENTHIYRIDEQRRVTVWAHDPLLGGSLVSLNSMVFTPDHKAVLTAVYLQPS
                     LVRVSVANPRDVRRVKLSGDIFMDGFNVLNGPDDLMMTSNNQLVVAFGSTLKRITPSD
                     ASWSAATVKSTRTIGGVTALVEAEGKLYGVNGQSVRFALGVPPNGFEIFEIDPARLR"
     gene            complement(5881..6576)
                     /locus_tag="VMZ53_34270"
     CDS             complement(5881..6576)
                     /locus_tag="VMZ53_34270"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUS33636.1"
                     /translation="MHKVDSKMEAGEPPPPELIQKMGQLVGRSLKAGIFKDGAGLHRS
                     ATRARVTFRGGKPHVERGPYAGDNELIASFALVSLPTGIERAIELATELGQAAGGREV
                     EVGPVVEGWDLNGSARPANTPFRFLLLLKGDASYEASGPAPAAVRTLLDRWKSDGLMQ
                     SEGSLKPSKHAARSRVVGGKRQWIDGPFAESKELIAGFSMLDLPSLEDVKRFTDEFAA
                     IIGDNEIDVREVA"
     gene            complement(6603..7004)
                     /locus_tag="VMZ53_34275"
     CDS             complement(6603..7004)
                     /locus_tag="VMZ53_34275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUS33637.1"
                     /translation="MAKVTGIGGVFFKAKNDPKELAAWYEKNLGMKLESFGAAILRWP
                     LDTAEDKGVTVWHLAKADSDWFSPSQSSFMINYRVDDMAGLIEQLRKNDVKLVKGPEY
                     HENGAFAWVMDPDGNKIELWEPKIWDDKNKK"
     gene            7084..7926
                     /locus_tag="VMZ53_34280"
     CDS             7084..7926
                     /locus_tag="VMZ53_34280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUS33638.1"
                     /translation="MTAALAVSDLRKTYGRTEALRGVALEVGPGELFGLLGPNGAGKS
                     TLVKIACGLVRPTSGHASVAGARAGTLAAQRSLGYLAELFRFPDWQTADEVLALHQKL
                     AHSTRGAAERAELLELVGLTDATHQRLGGMSKGMQQRLGIAQAMIGSPKLLLLDEPTS
                     ALDPAGRRTVRRLLEELKSRGVAVLLNSHLLSEVELVCDRVAIIARGQVVAAGTPAQL
                     SKAGGVEVETASGKRVFADAAREDAPRIVRELVTAGEDVFSVRVLTSSLEDAYLEAVG
                     DQGR"
     gene            7923..>8681
                     /locus_tag="VMZ53_34285"
     CDS             7923..>8681
                     /locus_tag="VMZ53_34285"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUS33639.1"
                     /translation="MSAFAIARVGLQEALRRRVFIIVAILTVLYLGLYALGTWQAFKE
                     VHGHSMGGANADIITAATLLGLNMFGTMFLGTILAVFLTLNTVRGDAERGLLQPLLVR
                     PVPRVTFLLARFAAAAVVCGAYVIIVFLIAALITYHFGGWWPDRVVEPALSLAAAVAI
                     LCMLSLAGSVFLSATANGIAVFMIFGAGLVAGLLGQIGEAIGSETLQNVSRISCWILP
                     FEALYQSALGRLTTDTFGITRIAIDLGPFGGAQNF"
BASE COUNT         1406 a         2895 c         2769 g         1611 t
ORIGIN      
        1 ccggtgaatg cgcccttctc gtgaccgaac agctcgctct cgatcagcga tggcggaatc
       61 gacccgcaat cgacgacgac gagcggcttc tgcgcgcgac tgcttgcggt gtggatcgcc
      121 tccgcgagca cgcccttgcc ggtgccggtc tcgccctcga tgagcaccgt cgaatcggtc
      181 gcggcgatcc gcggcaggac ggagaacagc cgacgcatcg caacgcttcg tccgagtgca
      241 cggccgaaga attcgtcgtc gctgagcggc tcgtcgagct gctcggacat ctcgtcgtag
      301 cggatgcgcg tcatgccgac ggtgagcacc gcgcccgact tcaaatatgc agagccaacg
      361 cggaacccgc cgagcgtcgt gccgttcgtg ctgccgaggt cgcgcagctg cacgccctgc
      421 ggatcgacct gcagcacgaa gtggtaacgc gacaccgtcg gatcgctgag caccagcgcg
      481 ttcgactcgg ccgtaccgac ggacacctct gctgccgacg tgcagacgtg ctccttgcct
      541 ttgtcgaggc cgtcgagcac gcgcaacgcg aagcgctgga accggagctc cttcacgctt
      601 ccacgcagaa ggctcgtact cattccgggc gatgctagca tggtgccttc gatggtcgcg
      661 cccgagcgct atcagctcct cggtccgctc ggcaccggtg gcatggccga ggtgctgctc
      721 gcgaagacgc gcggcccgaa cgggttcgag cggctgctcg cggtcaagcg catcctgccc
      781 gagcgcgcga aagaccccga tgcggtgcgc atgctcgtcg acgaagcgcg caatgccgct
      841 gggctctctc accatcgaat cgtgcaagtc ctcgacatcg agctgcgcga cggcgtcgtg
      901 ctctatgcga tggagtatct gcacggccag acgctcgagg cgctgctcgg caaggctcgg
      961 cgccttccgc tcgacgcatc gctcgccatc tgcatcgccg ttgccgacgg tctgcaccac
     1021 gcgcacgagc ggtcgtcgcc gatcattcat cgcgatgtcg cgccgagcaa cgtcatggtg
     1081 ctctacgacg gcaacatcaa gctgatcgac ttcgggatcg caaaggccgc caacaatctc
     1141 agcaacacgg tgttcggcac cttcaaaggc aggctcggct actcgtcacc cgagcaagta
     1201 cgctgcgaag cggtcgatcg ccgcaccgac gtctactcgc ttgccgtcat gctctacgag
     1261 ctgacgaccg gccggccggc attctccgcg cacgacgagc cgcacatgct cgacctgatg
     1321 gagaaggcgc gcatcacccc gccgcgcgag ctcgacgcac agtatccggc cgagctcgaa
     1381 gcgatcgtca tgaaggggct cgcgcgtgac cgcaatgcgc gatacgcgac tgcggccgcg
     1441 atgcagctcg acctcgaagc attcgcgcgg cgcgccgatc tcagcctcac ggacctcacg
     1501 atctctcgcc tgatggcgaa gctgttcgcg acggagctcg agccgtggca ccgcgcgcgg
     1561 aacggcggca tgaccctcga agaccacgtc atccaggcga cgctgcgcac gctgcccccg
     1621 tcgacgcatg actcgatcga ggagcgtgcg acacagccac gcgttgcacg aagacgccgt
     1681 cgccgccgca accgcatggt gatcggcatc gcggtcgtcg tcgcgttgtt cgccgcggct
     1741 tatctgttca cgcgctgggt gctcgtcgat ccgccgtcgc tcaaggctgc atctccgtct
     1801 ggatgaacgt gcccgcgacg atattgatgt tgtgcgtccg caggttcatg ccgccgacgg
     1861 tcgccgacag catcacgttg ccaaccggca ggttcgcgac gagcacgacg ccgagcgtat
     1921 cggtcgacgt cgcggtctga gagggaacct gctgcgcgaa gtagcgaatc gtgcccgccg
     1981 gagatgtcgt gaccgttccg ccggcgagcg cctttccatt gcagtcatcg acggtgagga
     2041 tgatgtggcc tttggccgga tccagcgtga tgccagcggc catcgccaat cgatcggcat
     2101 cggtctgcga gaacagttgg atcgcgccga cggcgggaaa cgagatgctg tgcgtgacct
     2161 tgtacgcggg gtagtagttc gtcggaaaga ggccgtttgc ggagaccgcg agatacagat
     2221 cgagcggggt gccgccggtg ttctgcgact ggcgaaacat gccactcgca tcggtgtgcg
     2281 cgaggaagat cgacgtcggc gtcccagcga gctgcccggc gacggacgca ttttggagcg
     2341 gcgttccagg gccggcgacc acgacggtgc cgctgatcac cgcgggattg tcggccttcg
     2401 tcggcgcggg cgcgttgaga cagccaaacg gcgtgccctg cgcgtcgatg agctctttct
     2461 ccggaaagct gcacgcggtg atcgctaacg cgcagacgac agcgcgcatc agaagtgacc
     2521 tccgacagca aagccagcgg tcgtatcgtc gacgaccggc acgatcgcga cgctatctgg
     2581 cgatgccggc ttgccgacgt accagagcac gccagccgtg atcagcgccg caccgccgat
     2641 cgcccagctg acgttcgcga cgagcgcatc ggtgcgcgcg gtcctgttca atgcaactcc
     2701 gttggcatcg gcgcacttga tcgaggggca tagctgatcc gactgctgct ccaagttgcg
     2761 tccgtagagt gcgaacgccg tcccgacgac gagcccgccg acgccgacca gcgcgagcgt
     2821 cgtcgcttcc gtctgatact tgtgctctag cgcgttcgga acacgcggaa gcttccgcgg
     2881 cctctcttcg atcggctcct cttgctcgtc gggtccttca cgcttctcga gcgttggcac
     2941 ctcgatgacc ttgttcttcg tcttcacggt gacggtctcg gaccagcgct tgtagccggg
     3001 cgcctgcgcg gtgatgcggt actcgtcggg atcgaccggc accttcgtgt cccagaggcc
     3061 aggatcgagc ggcaccttgt tgcgcttgat gacgagcccc tcgacgcgca cctcgggtgg
     3121 cacttcgatc gtcagataga cgagctggtc cgcgagcgcg ttcgcgcggg tctttgcttc
     3181 ggcagcgcgc ttgtcgttgt tcgtcttctt tgcgctcgcg gcggccttca gatacatcgc
     3241 ccacgccgac gcggtctggc cgttgagctc gcggcacttc gcgaggttga gctcggtgcc
     3301 cgacgcgggc tcgatgcggt cgctcgcctc gaacttctcg cacgcctcgt cgtattgctc
     3361 gagcttcatg aggcgctggc cttcgcggaa cagcacttcg gcttcggctg tctgtgcgct
     3421 cgcgttcgtc ggcactgcga tggacatcgc gagcgcagca acgacacagc tagaagcgat
     3481 cctcatacgt ttcggcgttc gtgtgcccct tcttcgtctt gcgaccaggg cgtttgaccg
     3541 gcgtcttaga tggtaccgcc gtcgtggccg gctcgcccac ttccacgtcg gagccttgca
     3601 cgctccccga tgcaccggtc tcgacgggca cggtttgggg cacgatcggc gccggcggcg
     3661 cgacgacagg gggcggcgta ggctcgatct cgacgcgtgg ctgcaccgcc ggcacgacct
     3721 tctcgctact gccaccgtgc gtcgtgaccg caagcacgat ccccgccgca atcgcgatcg
     3781 tcgcgatccc gatccacagg cgcagcggaa tcgggcgcgc atcggtgagc gactcgcctg
     3841 cggatgttcc gagcgtcgtg atcgcatctt cgcgcacttc tcgcagcgcg acgccttcgc
     3901 tcgacgagat cggcggagcg gccggtagct gggcgaacgc ggcctcgcac gcctcctgga
     3961 gctcggtcat cgagctgtag cgatcgtcgg gattcttcgc gaggcaacgc gcgacgattg
     4021 gatcgagcag ctccggcacg cgcggcgcga cgcgactcgg aaccggcgcc ggctcgcgca
     4081 agtgtgcagc gatgatcgca ccgacgcctt cgaggtcgaa cggcggccgg ccggtcagga
     4141 gatgaaacat cacgcagccg agcgaataaa catccgttcg gtgatcgatc cgccccgagt
     4201 cgttgcattg ctcgggagac atgtacgccg gcgttcccat cacggcgccc gtgcgcgtct
     4261 gaaagcgctg cgtcagctct tcatcgccga gcttcgcgat tccgaagtcg agaatcttcg
     4321 gccgctcgcc accaagcgcc tccttgtcgc ggaccatgaa gaggttctcg ggcttcagat
     4381 cgcgatgcac gatgcccatc gcatgcgccg cggcgagcga gcccgcaatt tgtcgcgtga
     4441 tgcgaacagc gtccgacgga tgcagggctc cacgattgcg aaggcgtgcg ctcagcggct
     4501 cgccatcgag aaactccatg atgaggtacg cggtgccatc ggtcgtgacg ccgaagtcgt
     4561 agacctggac gatgcccggg tcctccatcg ccgacgtcgc gcgcgcttcg gtgaagaatc
     4621 gttcgacgct ctcgcgctgc gtgctgcgct cgcgatggag caccttgatc gctgcgcgcc
     4681 ggccgatgag cgtgtgctcg ccgagaaaga ccgcgcccat gccgccctga ccgagctttc
     4741 gcaccacccg gtacgggccg aagttcactc cgggagcgta tcaacgaagg cgcgctggat
     4801 cgatctcgaa gatttcaaag ccattcggcg gtactccgag tgcaaagcgc acggactgcc
     4861 cgttgacgcc gtagagcttg ccctcggctt cgacgagcgc ggtgacgccg ccgatcgtgc
     4921 gcgtcgactt cactgtcgct gccgaccacg atgcatcgct cggcgtgatg cgcttgagcg
     4981 tcgagccgaa cgcgacgacg agctggttgt tgctcgtcat catgagatcg tcggggccgt
     5041 tcagcacgtt gaagccatcc atgaagatgt caccggatag cttcacgcgg cgcacgtcac
     5101 gcgggttcgc gacgctcacg cgcacgagcg acggctgcag gtacaccgcg gtgagcaccg
     5161 ctttgtgatc cggcgtgaac accatgctgt tgagactgac gagcgagcca ccgagcagcg
     5221 ggtcgtgcgc ccacaccgtc acgcggcgtt gttcgtcgat gcgatagatg tgcgtgttct
     5281 cgcgatccga gacgagcgcg gtgtcgccat cgagcagaac gtcgttgcac gccgcctctg
     5341 ggtttacttg cgtcagatcg atcgaagccg tgcgcgcacc gctctcgaga tcgaagatcc
     5401 agatcgtgcc gagcgagtct ttcgtcgtgc agacccacag cgcgcggcgc tgcgcatcga
     5461 cctgcatgcc gagcgtgtag cgcttgtctt caccagtgcc ggcgtagaac agcgactcgc
     5521 ggccatcggc gtcgacgcgg gtgacggtgc cgtgctgaag gctcccgacg tagaacgcgc
     5581 gatcgaccga gtcgtatgcg ccgccctccg ggaacagctt cttgtcgctg aacgtgtgca
     5641 cgacgcggcg gctggcctgc gattcgtccg ccttgccgtc gagcgcggtg tcatcgaatc
     5701 cgtcgtgttc agacacctcg ttggcgtctt ctgcacaggc ggcgagcgaa gcgacgagag
     5761 cagtagccag gacgatgcgc atggcacgtt cttcatcaga gtccgtgcca ggcggaccgc
     5821 ccacaagccc gcgcacttag gcaggagcgt ggcgacgcga gttgccgccc gaaaatgacg
     5881 ctacgcgact tcgcgaacgt cgatctcgtt gtcaccgatg atcgccgcga actcgtcggt
     5941 gaagcgcttc acatcctcga gcgacggcaa gtcgagcatc gagaatcccg cgatcagctc
     6001 cttcgattcg gcaaacggcc cgtcgatcca ctgccgcttg ccaccgacga cacggctgcg
     6061 cgcagcgtgc ttgctcggct tcagcgagcc ttcgctctgc atgaggccat cgctcttcca
     6121 acgatcgagg agtgtgcgca cagccgccgg cgcggggccg ctcgcttcgt acgacgcatc
     6181 gcctttgagc agcagcaaga accgaaacgg cgtgttcgca ggccgcgcgc ttccattcaa
     6241 atcccagcct tcgacgacgg ggccgacttc gacttcgcgc ccgcccgctg cttggccgag
     6301 ctctgttgca agctcgatcg cgcgctcgat gccggtcggc agcgatacca acgcgaagct
     6361 cgcgatcagc tcgttgtcac ccgcatatgg cccacgctcg acgtgtggct taccgccgcg
     6421 aaacgtgacg cgcgcacgcg tcgcgctgcg atggagtcca gcgccatcct tgaagatgcc
     6481 tgctttgagc gagcggccca cgagctggcc catcttctga atcagctcgg gcggcggtgg
     6541 ctcaccggct tccatcttcg agtcgacctt gtgcatgcac atgaacagca tggcgctctc
     6601 cgctacttct tgttcttgtc gtcccagatc ttcggttccc acagctcgat cttgttgccg
     6661 tcaggatcca tcacccacgc gaatgcgccg ttctcgtgat actcggggcc cttgacgagc
     6721 ttcacgtcgt tcttgcgcag ctgctcgatg aggccggcca tgtcgtcgac gcgatagttg
     6781 atcatgaagc tcgactggct cgggctgaac caatcgctat cggccttcgc gaggtgccag
     6841 accgtgacgc ccttgtcctc agcggtgtct agcggccacc gcaggatcgc agcgccgaac
     6901 gactcgagct tcatgccgag atttttctcg taccaagctg cgagctcctt cggatcgttc
     6961 ttcgctttga aaaacacacc accgatgcct gtgacctttg ccatgccagc tcgttaccac
     7021 gatcgcggaa caaagatcgc acgcacgttg ttgcatcgac caacgcgcgt taaggagtag
     7081 ctcgtgaccg ctgcgctcgc cgtctccgac cttcgcaaga cctacgggcg caccgaagcg
     7141 ctgcgtggcg tcgcgctcga agttggaccg ggcgagctgt tcggtttgct cgggccgaat
     7201 ggcgccggta aatcgacgct cgtgaagatc gcgtgcgggc tcgtgcgccc gacgtcaggt
     7261 catgcgtccg tcgcgggggc acgcgcaggc acgctcgccg cgcagcgctc tctcggatac
     7321 ctcgcggagc tgttccggtt tcccgattgg cagaccgccg acgaagtgct cgcgcttcat
     7381 cagaagcttg cacattcgac gcgcggagcc gccgaacgcg cggagctgct cgagctcgtc
     7441 gggctcaccg acgcgactca ccaacggctc ggcgggatgt ccaagggcat gcaacagcgg
     7501 ctcggcatcg cgcaggcgat gatcggctcg ccaaagctgc tgctcctcga cgaaccgacg
     7561 agcgcgctcg atccggcggg ccgccgcacc gtgcgtcgat tactcgaaga gctgaagtcg
     7621 cgtggcgtcg cggtgttgct caactcgcac ctgctttccg aggtcgagct cgtctgcgat
     7681 cgcgtggcga tcatcgcgcg agggcaggtc gtcgctgcgg ggacaccggc gcagctctcg
     7741 aaagcaggtg gcgtcgaagt cgagaccgcg tccggcaagc gcgtgtttgc ggatgccgcg
     7801 cgcgaggatg cgccacgcat cgttcgtgag ctcgtcactg caggcgaaga cgtgttctcc
     7861 gtgcgcgtgc tgacgtcatc actcgaggac gcttatctcg aagccgttgg agatcaaggt
     7921 cgatgagcgc gttcgcgatc gctcgcgtcg gactgcaaga agcgctgcgc cgccgcgtct
     7981 tcatcatcgt cgcgatcttg acggtgctct atctcggtct ctacgcgctc ggtacgtggc
     8041 aagcgttcaa ggaagtccac ggccactcga tgggcggcgc gaacgccgac atcatcacgg
     8101 cggcgacgct gctcggcctc aacatgttcg gcacgatgtt cctcgggacg atcctcgcgg
     8161 tcttcctgac gctcaacacc gttcgcggcg atgcggagcg cgggctgttg caaccgctgc
     8221 tcgtgcgtcc cgttccacgc gtcacgtttc tgctggcgcg ctttgcggcg gcggccgtcg
     8281 tatgtggcgc gtacgtgatc atcgtgttcc tgatcgcggc cctcatcacg tatcacttcg
     8341 gtggctggtg gccggatcgc gtcgtcgagc ccgcgctgtc gcttgccgcg gcggtcgcga
     8401 tcctgtgcat gctgtcgctc gcgggctccg tgtttttgtc cgccaccgcg aacggcatcg
     8461 cggtcttcat gatcttcggc gccgggctcg ttgcaggtct gctcggccag atcggtgaag
     8521 cgatcggttc cgagacgctc cagaatgtgt cgcgcatctc gtgctggatc ctgccgttcg
     8581 aggcgctcta tcagtccgcg ctcggccggc tcacgaccga tacgttcggc atcacgcgca
     8641 tcgcgatcga cctcggtccg ttcggcgggg cccagaattt c
//
LOCUS       DATLBZ010000704         7236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_10424026, whole genome shotgun sequence.
ACCESSION   DATLBZ010000704 DATLBZ010000000
VERSION     DATLBZ010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10424026"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1671)
                     /locus_tag="VMZ53_34290"
     CDS             complement(151..1671)
                     /locus_tag="VMZ53_34290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33640.1"
                     /translation="MGTPSDSDIDVGEPIEREGHETLLPSVTRSVLSTKSFVGDIDGR
                     DSFAAGEIDPPARPSAHGSVAPAGSIEAAVALEAEKVRGARSGDIVGGRYVVEGQLGR
                     GGMGRVLRVRHSALGKPFALKLIKSAIATNPKIRELFYREARLASALTHDNICSIVDF
                     GQDSGFGLFMVMELLEGQTVHARLRHSGRFPAKVACDIMWQVADATRFIHSRQILHGD
                     IKTENMFMVRTPAQRRLIKLLDFGLSRADLGHNEGIDGTPEYLAPERIEGAAASQASD
                     IYALGVVFFELLTGKLPYVGDTKEVFRQHRKEPFPMPSKFCDGEALDDRADDLVRRAT
                     AKDPAERHPDVGTFMYELRTLMQMLGMDSGGGGGGRRRALAPEAARERRDHDHSVKAS
                     AELFRSVSLPMACSDPSGKVRVANPAFLEFLGVAGGAGGLQLRDSALPEVYPTLFEDL
                     EQVSQTRRPIKRVIYLNEGGDRVIEAAVVLTAAPTGAEVTAGEVFIVLHPLRSLLS"
     gene            1709..2023
                     /gene="cyaY"
                     /locus_tag="VMZ53_34295"
     CDS             1709..2023
                     /gene="cyaY"
                     /locus_tag="VMZ53_34295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HUS33641.1"
                     /translation="MDEATFDQVARDELRAIEDAFSEIDPDEVEVSTSDGVLRLDLKD
                     GTKIVINSHRAARQIWMAAVASAWHFDPAGERWTATKTGEDLRPTLARLLRERIGLEI
                     RL"
     gene            2060..2428
                     /locus_tag="VMZ53_34300"
     CDS             2060..2428
                     /locus_tag="VMZ53_34300"
                     /inference="COORDINATES: protein motif:HMM:NF016291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HUS33642.1"
                     /translation="MAESQILSVIRVWAAVAWADGMLAESEAEGMRRLIRTADLTNDE
                     RSSALTFLDAKVDLPDLYLKNLNPESRRGIYRAACRMAVVDHVFAHAERKLLDRLQAL
                     LGIPLDIAQEIEADVPGMEV"
     gene            2486..3274
                     /locus_tag="VMZ53_34305"
     CDS             2486..3274
                     /locus_tag="VMZ53_34305"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUS33643.1"
                     /translation="MAAVSQAMMFTGLYPLVPHALRVVTRRPAAAVPLRLGGTVRTAL
                     AEWGMSVAVSAARPAGFFPLPGARQVGPRPVIVLHGYAMNRANFLPLAYRLARAGLGP
                     IVGFEYWTLGRTAAAAKQLAWFVDQVIARTGSPQVDIIGHSMGGVVGRYYVTLLGGDG
                     AVANLITLGSPHFGTDVSRIGIGAPTRELIFGSKLVQRLAAAPPPRDTRVLTILSHAD
                     ALVPAHAQMALEIPRAERIVYDDLGHVALLGSRRVAKEIIRRLK"
     gene            complement(3275..3958)
                     /locus_tag="VMZ53_34310"
     CDS             complement(3275..3958)
                     /locus_tag="VMZ53_34310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33644.1"
                     /translation="MSTEADSPIPVAQSNDELTFTALRAGGTKVRIDAPDGTTYATFT
                     VYAAEIDHIIAMPTVHESVPPEVTELAFAATYETRAEVRIISNSTMLLDDNIEIMPPP
                     GGVVLGGSSFPYFFTFDQAVLGESTISLTSGGKTFTAPFVHLETADTINRIVPPYPQV
                     VTCFSAMNGTRFVQGLTWTYVLEGNHVDSAHSNCLEVDAADDIDATGGVGVDVSAGGA
                     TNHFAISLH"
     gene            complement(4374..6218)
                     /locus_tag="VMZ53_34315"
     CDS             complement(4374..6218)
                     /locus_tag="VMZ53_34315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33645.1"
                     /translation="MSTPQHPLVELATGALQRLAKIPRYGNGHEESAAGLERMWVKAP
                     LVVAIGGPVQARTELWNFLCNKKVFDATSRPNDSAPLKVRRGKETRFKAVRDDGSVEE
                     HLLPPEQADDDALRMRAIATKAEVGERKLALERVEKALPRVARARPRGLMIWLWPLWW
                     LFTRRQRRILAERRFTEIAYDQACDARDLATKELEETEARILRERGRFFESLRALASG
                     PPLGSNVRDVLLELGDGPLPEGVELIELARTTKASDVDAIFLVERDTLHAPHGADAAA
                     VAIGKTTDVIPQLLLLLGRARSLGIARRAHEEITEPLTNLDDEITETEEMFRVRIERL
                     EAMQIMDAEEFARAELAKVRPQVSQSIHAVIEHAAHHLGGELQRLGDEWQRAIAIAQN
                     NDDLKLAVQRIEVSAPLDAKRIAEETRMLAVGGAAGAAHDVFPEMMGALKAHGLEEPP
                     PREAPQLLPLEMLPSLTNASPSKLSGAGSWLGGLFRSFESKRADVGTKADARVKHLRD
                     VAQAEILDVEPKLRAVIEQTLYQMMLAAIERQVAWLDRTLTLEREAVSREGTALAPLA
                     RMRDRLRHDHQKLAEGIEQIEKADAGIAAAAAAVTITQERATTDVDDD"
     gene            complement(6215..6610)
                     /locus_tag="VMZ53_34320"
     CDS             complement(6215..6610)
                     /locus_tag="VMZ53_34320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33646.1"
                     /translation="MGRLRVVLAFGLLAGAAGCPHRGTSAGGGGPAGFAITYPDAAHA
                     TAKTGKRFYAKPEGRCNYDDGREARWTMTGTHLASGELPPGLTIEDGVIGGVPTAAGV
                     YHARIELRGVMCAGKAYDEQGVDIEITVK"
     gene            complement(6621..>7236)
                     /locus_tag="VMZ53_34325"
     CDS             complement(6621..>7236)
                     /locus_tag="VMZ53_34325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUS33647.1"
                     /translation="TSPYPVDFTADVIAAIAEEPKICKYVHLPVQSGSDVVLERMRRG
                     YTVADYIAIVKALRAAMPQIALSTDILTGFSGETEEDHQRTLDLMREVRFDSAFMFAY
                     SERDLTFAAKKLPDDVPPAVKQRRLSEIVALQERTSAEVFAAHVGRNERVLVVGPSKR
                     DPKQLIGRTDGFKTVILPTGVGEVGGFVDVTIERATMATLFARA"
BASE COUNT         1174 a         2328 c         2454 g         1280 t
ORIGIN      
        1 agagggtgaa gaccacggcc tgaaggacta caaagacatc gacgacaacg agctcgacat
       61 gcagacgcgc acgatcgtcg ccgcgcagct actaccggcg cagcaagcga cgcacaaccg
      121 catcagctcg ctgaaaaaac agctgcgcag ctaagacagc agcgagcgca gcgggtgaag
      181 cacgatgaag acttcgcccg ctgtgacttc cgcgccggtc ggggcagcgg tgagcacgac
      241 ggcggcttcg atcacgcggt cgccaccttc gttgagatag atgacgcgct tgatcgggcg
      301 gcgcgtttgc gagacctgct cgaggtcttc gaacagcgtc gggtagactt ccggcagcgc
      361 gctgtcgcgg agctgcaggc cgcctgcccc gccggcgacg ccgaggaact cgaggaacgc
      421 ggggttcgcc acccgcacct tgccggatgg atcggagcac gccattggca gcgagaccga
      481 gcggaagagc tcggcgctgg ctttgacgct gtggtcgtga tcgcggcgtt cgcgcgcggc
      541 ttcgggcgcg agcgcgcgac gacgaccgcc gccaccgccg ccggagtcca tgccgagcat
      601 ttgcatcagc gtgcgcagct cgtacatgaa cgtgccgacg tcgggatggc gttcggcggg
      661 gtctttcgcg gtcgcgcggc ggacgagatc gtcggcgcga tcgtcgagcg cttcaccgtc
      721 gcagaacttc gacggcatcg ggaacggctc tttgcggtgc tggcggaaca cttccttcgt
      781 gtcgccgaca tagggcagct tgcccgtgag cagctcgaag aacacgacgc cgagcgcgta
      841 gatgtcgctt gcttgcgatg cggcggcgcc ttcgatgcgc tcgggcgcga ggtattcggg
      901 cgtgccgtcg atgccttcgt tgtggccgag atcggcgcga gagagaccga agtcgaggag
      961 cttgatcagg cggcgctgcg cgggcgtgcg caccatgaac atgttctcgg tcttgatatc
     1021 gccgtggaga atctgccggc tgtggatgaa gcgcgtcgcg tcggcgactt gccacatgat
     1081 gtcgcacgcg actttggccg gaaaacggcc gctgtggcga aggcgcgcgt ggacggtttg
     1141 gccctcgagc agctccatga ccatgaagag gccaaagccg ctgtcttggc cgaagtcgac
     1201 gatcgagcag atgttgtcgt gcgtgagcgc cgaggcgaga cgcgcttcgc gatagaagag
     1261 ctcgcgaatc ttggggttcg tcgcgatcgc gctcttgatg agcttcagcg cgaatggttt
     1321 gccgagcgcc gagtggcgaa cgcgcagcac gcggcccatg ccgccgcgac cgagctggcc
     1381 ttctacgacg tagcgaccac cgacgatgtc accggagcgc gcgccgcgaa ctttttcggc
     1441 ttcgagcgcg acggccgctt cgatcgatcc tgcgggcgcg accgagccgt gtgcagaggg
     1501 gcgcgcgggt ggatcgatct cgccagctgc gaagctgtca cgaccatcga tgtccccgac
     1561 gaagcttttc gtggaaagga cagaacgcgt caccgatgga agcagggtct cgtgaccctc
     1621 gcgctcgatc ggctccccta cgtcgatgtc actgtccgac ggagtcccca ttgcctccat
     1681 agtagcgaaa aacggctata aggtgcgcat ggatgaagcg acctttgatc aagtcgcccg
     1741 cgacgagctt cgggccatcg aggacgcctt ctccgaaatc gatcccgatg aggtggaagt
     1801 ttccacgagt gacggtgtgt tacgcctcga cttgaaagac ggaaccaaaa tcgtcatcaa
     1861 cagccatcgc gcggcgcggc agatctggat ggccgcggtt gcgagtgctt ggcacttcga
     1921 tcccgccggc gagcggtgga ccgccacgaa gaccggagaa gacctgagac ctaccttggc
     1981 gcggctgctt cgcgagcgga ttggcttgga aatccggctg taggcgcgat cgcccgagcg
     2041 ccggtggtat cgtcggctca tggcggagtc gcagattctc agcgtgatca gggtgtgggc
     2101 tgccgtggca tgggccgacg gcatgcttgc cgagtcggaa gccgagggga tgcgccgcct
     2161 gattcgcacc gccgatttga cgaacgacga gcgctcgtcg gcgctgacgt tcctcgacgc
     2221 aaaagtcgat ctacccgacc tctatttgaa gaacctgaat ccggaatcgc gccgcgggat
     2281 ctaccgcgct gcgtgtcgga tggcggtcgt cgaccacgtg tttgctcatg ccgagcgcaa
     2341 gctgctcgac cgcctgcagg cgctccttgg cattcccctc gatatcgcgc aggaaatcga
     2401 ggcggatgtg ccaggcatgg aggtctagct agacttgagc atgcagaagc gacactcgtt
     2461 gcgccatctc gcctggcgtt cgggggtggc ggcggtgtcg caggcgatga tgtttacggg
     2521 gctgtacccg ctcgtgccgc atgctctgcg cgtcgtgacg cggcgccccg ccgctgcggt
     2581 gccactgcgc ttgggaggga ccgttcggac ggcgctcgcc gagtggggca tgtcggtggc
     2641 tgtatccgct gcgcggcccg ccgggttctt cccgctgccg ggtgcccgtc aggtcggacc
     2701 gcggccggtg atcgtcctgc atggctacgc gatgaaccgc gcgaacttct tgccgctcgc
     2761 ctaccggctg gcgcgggcgg ggctcggacc gatcgtcggc ttcgagtact ggacgctcgg
     2821 ccgcaccgcc gcggccgcga agcagctcgc ctggttcgtc gatcaagtca tcgctcgcac
     2881 cggctcgccg caagtcgaca tcatcggcca ctcgatgggc ggcgtcgtcg gtcgctacta
     2941 cgtgacgctg ctcggcggtg atggcgcggt cgcaaatctc atcacgctcg gctcgccgca
     3001 cttcggcacc gatgtctcgc gcattggaat cggcgcaccg acgcgcgagc tgatcttcgg
     3061 cagcaagctc gtgcagcgcc tcgccgccgc gccgccgcct cgcgacacgc gagtactgac
     3121 gatcctctcg cacgccgatg cgctcgttcc cgcgcacgcg cagatggcgc tcgagatccc
     3181 gcgcgccgaa cgcatcgtct acgacgacct cggccacgtc gcgctgctcg gctcgcgtcg
     3241 cgtcgccaaa gagatcatcc gccgcctgaa gtaatcagtg cagcgagatc gcgaagtggt
     3301 tcgtcgcgcc gcctgcactc acgtcgacgc caacgccacc ggtcgcgtcg atgtcgtcgg
     3361 ctgcgtcgac ctcgagacaa ttgctgtgcg ccgagtcgac atgattcccc tcgagcacgt
     3421 acgtccacgt gaggccttgc acgaaccgcg taccgttcat ggccgagaag cacgtgacca
     3481 cttgcgggta cggcggcacg atgcggttga tcgtgtcagc ggtctcgaga tggacgaacg
     3541 gcgcagtgaa cgtcttgccg ccgcttgtca gcgagatggt cgactcgccg agcaccgcct
     3601 gatcgaaggt gaagaagtag ggaaacgagc tgccaccgag cacgacgccg ccgggcggag
     3661 gcatgatctc gatgttgtcg tctagtagca tcgtgctgtt gctgatgatc cgcacctcgg
     3721 cgcgcgtctc gtacgtcgcc gcgaacgcca gctccgttac ctctggaggc acgctctcgt
     3781 gcaccgtcgg catcgcgatg atgtgatcga tctcggcggc gtagacggtg aacgtggcgt
     3841 acgtcgtgcc atctggcgcg tcgatccgaa ccttcgtgcc gcctgcgcgc aacgcggtga
     3901 acgtgagctc gtcgttgctc tgggcgacgg ggatcggcga gtccgcctcc gtcgacacgg
     3961 tgaaagctcg gtcgaagggc accaggcccg acgcgctccg gcactggaca aaagtacgaa
     4021 gcgatccacc gacgatgatg ctgggcgaat aactcgggta cggcggcgcc tgttccggcg
     4081 tgaattcgag agtggcttgc aggcttctca cgaggcggag gcgccgccca acggattccg
     4141 gcgtcgcggc cggatcgaag caaggatcca cctgttgcgg ctgatccatc gggtcgggct
     4201 ccgcgcagcc cacggccgcg agcgtcatga agagacctac ggcacagcgc acgcgtgacc
     4261 ttcttgcagc gattggacct gcgtacccgc gcaagatcac ggtggctggc gacgaattat
     4321 tgtcgaatcg ccgaggcgtt gtgacgcaga tgtcgcggtc gacgacgcgg gctttagtcg
     4381 tcgtcgacgt cggtcgtcgc acgctcttgc gtgatcgtaa cggcggcggc agccgcggcg
     4441 ataccggcat cggctttctc gatttgctcg atgccttcag cgagcttttg gtgatcgtgg
     4501 cgcaggcgat cgcgcatgcg agcgagcgga gcgagggcgg tgccttcgcg cgacacggct
     4561 tcgcgttcga gcgtgagcgt gcgatcgagc cacgcgactt ggcgttcgat cgcggcgagc
     4621 atcatctggt agagcgtctg ctcgatcacc gcgcggagct tcggctcgac gtcgaggatc
     4681 tcggcttgcg cgacatcgcg caggtgcttc acgcgcgcat ctgctttggt gcccacgtcc
     4741 gcgcgctttg actcgaacga gcggaagagg ccgccgagcc agctccccgc gcccgacagc
     4801 ttcgacggcg atgcgttcgt cagcgacggc agcatctcga gcgggaggag ctgcggcgct
     4861 tcgcgaggtg gcggctcttc gaggccgtgc gctttcagcg cgcccatcat ctcggggaag
     4921 acgtcgtgcg cggcgcccgc tgctccgccg acggcgagca tgcgcgtctc ttcggcgatg
     4981 cgcttcgcgt cgagcggcgc cgacacttcg atgcgctgga ccgcgagctt gaggtcgtcg
     5041 ttgttctgcg cgatcgcgat cgcgcgctgc cattcgtcgc cgaggcgctg cagctctccg
     5101 ccgagatgat gcgcggcgtg ctcgatcacg gcgtgaatgc tctgcgagac ctgcgggcga
     5161 accttcgcga gctccgcgcg cgcgaattcc tcggcatcca tgatctgcat cgcttcgagg
     5221 cgctcgatgc gaacgcggaa catctcttcg gtctcggtga tttcgtcgtc gagattcgtg
     5281 agcggctcgg tgatctcttc gtgcgcacgg cgcgcaatgc cgagcgagcg agcgcgaccg
     5341 agcaggagga ggagctgcgg gatgacatcg gtcgtcttgc cgatcgcgac ggccgcggcg
     5401 tcggcgccat gcggagcgtg gagcgtgtcg cgctcgacga ggaagatcgc gtcgacgtca
     5461 ctggctttcg tggtgcgcgc cagctcgatg agctcgacgc cttcgggcag cgggccatcg
     5521 ccgagctcga gcagaacgtc gcgcacgttg ctgccgagcg gtggtcccga cgcgagcgcg
     5581 cgcaggctct cgaagaagcg accacgctcg cggagaatgc gggcttcggt ttcttcgagc
     5641 tccttcgtcg cgaggtcgcg cgcatcgcac gcttggtcgt acgcgatctc ggtgaagcgt
     5701 cgctcggcga ggatgcggcg ctggcggcgc gtgaacagcc accacagcgg ccacagccag
     5761 atcatgagac cgcgaggccg tgcacgggcg acgcgcggca gcgctttttc gacgcgctcg
     5821 agcgcgagct tgcgttcgcc gacttcggct ttggtcgcga tggcgcgcat gcgcagcgca
     5881 tcgtcgtccg cttgctcggg cggcagcagg tgctcctcga cgctgccgtc gtcgcgcact
     5941 gctttgaagc gcgtctcttt tccgcggcga actttcagcg gtgcgctgtc gttcggtcgc
     6001 gacgtcgcgt cgaacacctt cttgttgcag agaaagttcc acagctcggt acgcgcctgc
     6061 acgggcccgc cgatcgcgac gacgagcggc gccttgaccc acatgcgctc gagacccgca
     6121 gcgctctctt cgtggccgtt gccgtagcgc gggatcttcg cgagacgctg cagagcgccg
     6181 gtcgcgagct cgacgagcgg atgctgtgga gtgctcactt cacggtgatt tcgatatcga
     6241 cgccctgctc gtcgtacgcc ttgccggcac acatgacgcc gcgcagctcg attcgcgcat
     6301 ggtagacgcc cgccgccgtc ggaacgccgc cgatgacgcc gtcctcgatc gtgagccccg
     6361 gaggtagctc gccagatgcc aggtgcgttc ccgtcatcgt ccagcgcgct tcgcgcccat
     6421 cgtcgtagtt gcagcgacct tcaggcttcg catagaagcg cttgccggtc ttcgccgtcg
     6481 cgtgcgcagc gtcgggatac gtgatcgcga accctgcagg gccgccaccg cccgcactcg
     6541 taccgcgatg cggacagcca gctgcgcccg caagaagccc gaaggcaagg accaccctta
     6601 gccgtcccac ggaaaagatc ttacgcgcgg gcgaagagcg tcgccatcgt tgcgcgctcg
     6661 atcgtgacat cgacgaaccc accaacctca cctacgcctg tgggcaaaat aacggtcttg
     6721 aagccgtctg ttcggccgat gagctgcttc ggatcgcgtt tggagggccc tacgacgagc
     6781 acgcgctcgt tgcgacccac gtgggccgcg aagacctccg cggacgtgcg ctcctgcagg
     6841 gcgacgattt cagagagccg gcgctgcttc accgcgggcg gaacgtcatc cggcagcttc
     6901 ttcgcggcga acgtcaggtc gcgctccgag tacgcaaaca tgaacgcgct atcgaagcga
     6961 acctcgcgca tgaggtcgag cgtccgctgg tggtcctctt ccgtctcgcc ggagaagccg
     7021 gtcaggatgt cggtcgacag cgcaatctgc ggcatcgcag cgcgcagcgc tttgacgatc
     7081 gcgatgtagt ccgcgaccgt gtagccgcgg cgcatccgtt cgagcacgac atccgagccc
     7141 gactgcacgg gcagatggac gtacttgcag atcttcggct cctcggcgat cgccgcgatt
     7201 acgtccgcgg tgaagtcgac gggatacggc gacgtg
//
LOCUS       DATLBZ010000705         6186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6602464, whole genome shotgun sequence.
ACCESSION   DATLBZ010000705 DATLBZ010000000
VERSION     DATLBZ010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6602464"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VMZ53_34330"
     CDS             <1..553
                     /locus_tag="VMZ53_34330"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HUS33648.1"
                     /translation="LVAGDEVRAPAPGWFHLLVAPDHLVAPGDVIGELEGLGRVVRIT
                     APRVHGLVTLEKRPSLARRPVSFGDVLFRVATDVAIAGTSASAGGTGAATRAEAGLVF
                     RAPTSGRFYGRPSPDKPAFVEAGRELAAGATVCLLEVMKTFHRVTYGGADVPPRAKVR
                     EVLVADGADVNQGDALLALDPLA"
     gene            complement(556..2343)
                     /locus_tag="VMZ53_34335"
     CDS             complement(556..2343)
                     /locus_tag="VMZ53_34335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33649.1"
                     /translation="MGTHGDEGDPPTEPRTELPRTRSSAGAIVAEDATSVAGPYALGD
                     VIGRGGMGEVLLAHDRRIGRDVALKRLQATSPGQDELGRFMREARIQARLEHPAIVPV
                     YEMGRDTAGRPYFTMKRVVGQTLAELFAAKVTHRQRMLRAFAEVCRAIDYAHSRGVVH
                     RDLKPANIVMGEFGEIYVLDWGIARIVDTEQRIDMADIDTIEGVAPAAAEIIGTLGYS
                     SPEQLHAPNVDRPSDVYSLGAILFEILAGEPLHPRGKGAAAAKHSTMAGETIMSPALR
                     RPDRNVPPELDALCLAMLASKPATRPTARRSAEQIEEFLDGDRDLARRRVMAHDLVSL
                     ARDAVREGRSSDGMRAASRALALDPEARDAAELVMHVMLQPPTDVPPEVREELLRSDD
                     EAVSQHARAATPGYVIIAAFLPIIVWNGVRSWPIVLAMFALAVLMTLGAWDLTRRPRR
                     SLTHMIAYAVGNSLLLAIMGRLASPLLVVPALVAFVTGSVVTYPTFLVRKWLLIAIML
                     AGFLAPLGLEALGVLAPTWRLDDNGFVAFGDAMRLHGAAGIVTIFAGALAIMLMAGLQ
                     SARIGNASRAAHHRLVLQAHRLRQLLPAR"
     gene            complement(2381..2454)
                     /locus_tag="VMZ53_34340"
     tRNA            complement(2381..2454)
                     /locus_tag="VMZ53_34340"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2418..2420),aa:Met,seq:cat)
     gene            2563..2633
                     /locus_tag="VMZ53_34345"
     tRNA            2563..2633
                     /locus_tag="VMZ53_34345"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2595..2597,aa:Gln,seq:ttg)
     gene            2646..3044
                     /locus_tag="VMZ53_34350"
     CDS             2646..3044
                     /locus_tag="VMZ53_34350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33650.1"
                     /translation="MKLDKSCALFFATKIDSKLREGLALSKPGDKKYFDGSSEEFLRV
                     MEIGDEKDKERWIGKVIKPGPAVTEVDDIQRNILSILRRVAPNARIPASAVKIFVLQG
                     ANAVAAVIDDEPEEGDELDDPASSGPYITY"
     gene            3105..4808
                     /locus_tag="VMZ53_34355"
     CDS             3105..4808
                     /locus_tag="VMZ53_34355"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS33651.1"
                     /translation="MTTPEPRKSGLLELPRGTAIGKYEILRKLATGGMAEIYLARVSG
                     TAGFEKLVVLKRILPTIAEDPRLVQMFLDEARLAATLRHPNIADVYDVGDDDGIVFFT
                     MEFVHGQDVRTVRQDAKKRNDPVPLGVALAIVHGTASALEHAHELTGPDGKHLGLVHR
                     DVSTSNVMVSYDGAVKLLDFGIARASSSTHKTQTGMLKGKIAYMSPEQCRGTPLDRRS
                     DLFSLGIVMFELTVGRRPFRGDTDFAIMDQIVYQGAPKPSSIRPDYPPELEAIVMKLL
                     DRNPALRYPTAEDLLHDLDEYIGANGLWQSPRAIGKYMRTLFADKIVAWEQAEQEGVS
                     FAQHVAQSITSQSQRVELMTPSKSFPGVEPRPSQRHMLAHAPTAAQDVVAPPPEPAAP
                     LPVAKLESAPVAYPSIRPSRAATFFKVLLALLVLGGAGAAGFILTQNRQQTEPTAKDE
                     PAPAPAPVPAPVPAPAVAEEPKVEQPKEEPKVEEPKVEEPKVEEPKVEAKTEEPAVEA
                     KVEQPVKKTTGKHVVKKTTTKKTTTKTDATKTDATKVDKTPPKQDPSKPFDPDSPFLP
                     Q"
     gene            complement(4830..5366)
                     /locus_tag="VMZ53_34360"
     CDS             complement(4830..5366)
                     /locus_tag="VMZ53_34360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33652.1"
                     /translation="MSQPTQPPPRVIKTLGTLNLVFAGLGLLGGAMTYAMYFGGLKIA
                     PGPDPLREAALGSPAYMSFLRVSMVAGFIASLVLGASGIGLHRMKEWGRKAALAYAVY
                     AIVGAIAGIVMLAKYVLPALSKLHGPAAQGGVIGGMMGGVIAISYPIVILVFMNRRNV
                     REAFARANEPPVPPAQLR"
     gene            5397..6152
                     /locus_tag="VMZ53_34365"
     CDS             5397..6152
                     /locus_tag="VMZ53_34365"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUS33653.1"
                     /translation="MCYERRLTDEITIDALTSGWTIVQRKKGHRHSTDDLLTGWYAGE
                     IAPAATRLLDLGAGIGGVGLIALWRAPASATLVAIEAQDVSFGLLQQNIARNGLDLRV
                     RAIHGDLRGVRFHGLFDLVTGSPPYWDVSQGVVPADSQKAHARFELRGDVRDYCIAAR
                     AALDDNGRFVFCFPTVQRERAEAAVAAAELAVIRSRDVIPRAGAEPLFTLFACRRAED
                     SDEPHVREPDHVVRDEHGEPTPMHRAARAALGF"
BASE COUNT         1094 a         2078 c         2048 g          966 t
ORIGIN      
        1 cctggtcgcc ggcgacgaag ttcgcgcgcc cgcaccgggc tggttccacc tgctcgtcgc
       61 gcctgatcat ctcgtcgcgc ccggtgacgt gatcggcgag ctcgaagggc tcggccgcgt
      121 cgtccgcatc accgcgccgc gcgtgcacgg tctcgtgacg ctcgagaagc gcccgtcgct
      181 cgcgcgccgc cccgtcagct tcggcgacgt gctgttccgc gtggccacag atgtcgcgat
      241 cgcggggacg agcgcatcgg ctggcggtac cggcgcggct acgcgggcag aggccggcct
      301 cgtattccgc gcgcccacga gtggtcgctt ctacggtcgg ccatcgcccg acaagcccgc
      361 gttcgtcgaa gcagggcgcg agctcgccgc cggcgcgacg gtgtgcttgc tcgaagtgat
      421 gaagacgttc caccgcgtga cgtacggcgg tgccgacgtg ccgccgcgcg cgaaagtccg
      481 cgaggtcctc gtcgccgacg gcgccgacgt caatcagggc gacgcgctac tcgcactcga
      541 tccgctcgcc tgatatcagc gcgcgggtaa gagctggcgc aggcgatgag cctgcagcac
      601 gagtcgatga tgcgcagcgc gactcgcgtt gccgatgcgc gcggattgca gaccggccat
      661 caacatgatc gcgagcgcgc ccgcaaagat cgtcacgatg ccggcggcgc cgtggaggcg
      721 catcgcgtcg ccgaatgcga cgaagccgtt gtcgtcgaga cgccacgtcg gcgccagcac
      781 gccaagcgct tcgaggccga gcggcgcgag aaagccggcg agcatgatgg cgatgagcag
      841 ccacttgcgc acgaggaacg ttggatacgt cacgaccgat ccggtcacga acgcgaccag
      901 cgccggcacg acgagcagcg gtgacgcgag gcgtcccatg atcgcgagca agagcgagtt
      961 gccgactgcg taggcgatca tgtgcgtcag cgatctgcgc gggcgccgcg tcaggtccca
     1021 cgcaccgagc gtcatcagaa ctgcgagcgc aaacatcgcg agcacgatcg gccacgagcg
     1081 gacgccgttc cacacgatga tcggcaagaa cgcggcgatg atgacgtagc ccggcgttgc
     1141 cgcgcgcgcg tgctggctca cggcttcgtc gtcggaacgc agcagctctt cgcgcacctc
     1201 gggtggcacg tccgtcggcg gctgcaacat gacgtgcatg acgagctccg cggcgtcgcg
     1261 tgcttcgggg tcgagcgcta gtgcgcgact cgcggcacgc ataccgtcgg agctgcggcc
     1321 ttcgcggacg gcgtcgcgcg cgagcgacac gaggtcgtgc gccatcacgc gacggcgcgc
     1381 gagatcgcga tctccgtcga ggaattcttc gatctgctcg gcgctgcgac gcgcggtcgg
     1441 ccgcgtggcg ggtttgctcg caagcatcgc gaggcagagt gcgtcgagct cgggcggaac
     1501 gttgcgatcg gggcggcgca gcgcgggcga catgatcgtc tccccggcca tcgtgctgtg
     1561 ctttgcggcc gcggcgccct tgccgcgtgg atgcagcggc tcacccgcga ggatctcgaa
     1621 caggatcgcg cctagcgagt agacgtccga cggcctgtcg acattgggcg cgtgcagctg
     1681 ctcgggtgag ctgtaaccga gcgtgccgat gatctccgcg gcggccggtg cgacgccttc
     1741 aatcgtatcg atgtcagcca tatcgatgcg ctgctcggta tcgacgatgc gcgcgatgcc
     1801 ccagtcgagg acatagatct cgccgaactc gcccatcacg atgttcgccg gcttgagatc
     1861 gcgatgcacg acgcctcgcg agtgtgcgta atcgatcgcg cggcacacct cagcgaaagc
     1921 acgcagcatg cgctgacgat gcgtgacctt cgcggcgaac agctcggcga gcgtctggcc
     1981 gacgacgcgc ttcatcgtga agtacgggcg cccggcggta tcgcgcccca tctcgtagac
     2041 cggcacgatc gctggatgct cgagacgcgc ttggatacgt gcctcgcgca tgaagcggcc
     2101 gagctcgtcc tggcccggcg acgtcgcctg caagcgcttg agcgcgacat cgcgaccgat
     2161 gcgtcgatcg tgcgcgagca gcacctcgcc cataccaccg cggccgataa cgtcaccgag
     2221 cgcgtacggc cccgccacgc tcgtcgcatc ttcagcgaca atcgcgccgg cggacgagcg
     2281 cgttcgcgga agctccgtgc gtggctcggt cggcggatcc ccctcgtcgc catgcgtgcc
     2341 catgcgggca tcgtactcac gcgaggatga tgcggcgaag tggctggggg cggacttgaa
     2401 ccgccgacct tgggcttatg aaacccctgc tctaaccggc tgagctaccc agccttgggt
     2461 agggcggaga aactagtggg tcgcagctaa gctgtcaagc tcccaatgaa aaccggggga
     2521 tgggtagcgt cgttgctagt ttccggctaa gttctggcca gctcggggat cgtctaacgg
     2581 taggacagcg gtttttggtg ccgcgtatct aggttcgaat cctagtcccc gaacgaaggc
     2641 aaacgatgaa actcgacaag agctgcgcgc tgttcttcgc gacgaagatc gacagcaagt
     2701 tgcgtgaggg tctcgcgctc tccaagccgg gagacaagaa gtacttcgac ggtagctccg
     2761 aagaatttct gcgcgtgatg gagattggtg acgagaagga caaggagcgc tggattggca
     2821 aggtgatcaa gccgggaccg gccgtcaccg aggtcgatga catccagcgc aacatcctgt
     2881 cgattcttcg cagggtcgcg ccaaacgcgc gtatccccgc gtccgccgtg aagatcttcg
     2941 tgctccaagg tgcgaacgcc gtggcggcgg tcatcgatga cgagcccgaa gagggtgacg
     3001 agctcgacga cccggcttcg agcgggccgt acatcaccta ctagtgggcg tgtgatggtc
     3061 cgcggccgcg ttgcggtgcg cgtgttacgc ttttagcggg gattgtgacg acgcctgaac
     3121 cgcgtaaatc aggcttgctg gagctgccgc gcggcaccgc gatcggcaag tacgaaatcc
     3181 ttcgcaagct cgcgacgggt ggcatggccg agatttatct cgcgcgcgtg tccggcaccg
     3241 cgggcttcga gaagctcgtc gtgctcaagc gcatcctgcc gacgatcgcc gaggacccgc
     3301 gcctcgtgca gatgttcctg gatgaagcgc ggctcgctgc cacgctccgc catcccaaca
     3361 tcgcggacgt gtacgacgtc ggcgacgacg acggcatcgt gttcttcacg atggagttcg
     3421 tgcacggcca agacgtgcgc acggttcgcc aggacgcgaa gaagcgcaac gaccccgtgc
     3481 cgctcggcgt cgccctcgcg atcgtgcacg gcaccgcatc agcgctcgag catgcgcacg
     3541 agctgaccgg ccccgatggc aagcatctcg gcctcgttca ccgcgacgtg tcgacgagca
     3601 acgtcatggt gagctacgac ggcgccgtca agctgctcga tttcggcatc gcgcgcgcgt
     3661 cgtcttcgac gcacaagacg cagacgggca tgctcaaagg caagatcgcc tatatgtcgc
     3721 ccgagcagtg cagaggcacg ccgctcgacc gacgaagcga tctgttctcg ctcggcatcg
     3781 tgatgttcga gctcaccgtc gggcgccggc cgttccgcgg cgacaccgac ttcgcgatca
     3841 tggatcagat cgtgtaccag ggcgcgccga agccctcgtc gatccggccc gactatccgc
     3901 ccgagctcga agcgatcgtg atgaagctcc tcgatcgcaa cccggcgctg cgctatccga
     3961 cggccgagga tctgctccac gacctcgacg agtacatcgg cgcgaatggc ctctggcaat
     4021 cgccgcgtgc gatcggcaag tacatgcgca cgctgttcgc cgacaagatc gtcgcgtggg
     4081 agcaagcgga acaagaaggc gtgtcgttcg cgcagcacgt cgcacagtcg atcacgtcgc
     4141 agagccagcg cgtcgagctg atgacgccat cgaagtcgtt ccctggcgtc gagccgcggc
     4201 cgtcgcagcg tcacatgctc gcgcacgcac cgacggccgc gcaggacgtg gtcgcgccac
     4261 cgcccgagcc cgccgcgccg ctgccggtgg cgaagctcga gagtgcaccg gtcgcgtatc
     4321 cgtcgatcag gccgagccgc gcagcgacgt tcttcaaggt gctgctcgcg ctcctcgtgc
     4381 tcggcggcgc cggtgcggcg ggcttcatcc tcacgcagaa tcggcagcag acggagccca
     4441 cggcgaagga cgagccagcg ccagcgcccg ctcctgttcc cgctcctgtt cccgcgcctg
     4501 cagttgcgga agagccgaaa gtcgaacagc cgaaagaaga gcccaaggtc gaggaaccga
     4561 aagtcgaaga gccgaaggtc gaagagccca aggtcgaagc gaagaccgag gagcccgccg
     4621 tcgaggcgaa ggtcgaacag ccggtcaaga agacgacggg caagcacgtc gtgaagaaga
     4681 caacgacgaa gaagacgacg acgaagaccg acgcgacgaa gaccgacgcg acgaaggtcg
     4741 acaagacgcc gccgaagcag gatccgagca agccgttcga tcctgactcg ccgttcttgc
     4801 cgcagtagct cagcgcgcgc ttgccgtagc tatcgtagct gcgcgggagg taccggcggc
     4861 tcgttcgcgc gcgcgaaggc ctcgcggacg ttgcgccgat tcatgaacac gagaatcacg
     4921 atcggatacg aaatcgcgat cacgccgccc atcatgccgc cgatcacgcc gccttgcgct
     4981 gcgggcccgt gaagcttcga gagtgcaggc agcacgtact tcgcgagcat cacgatgcct
     5041 gcaatcgcgc caacgatcgc atacaccgcg tacgcgagcg ccgctttgcg gccccactcc
     5101 ttcatacgat gtaggccgat gcccgatgcg cccaggacga ggctcgcgat gaagcccgcg
     5161 accatcgaga cgcgtagaaa ggacatatac gcgggcgagc ccagcgcggc ttcgcgaagc
     5221 ggatcaggac ccggcgcgat cttcaaaccg ccgaagtaca tcgcgtacgt catcgctccg
     5281 ccgagaaggc cgagtcccgc gaacacgagg ttgagcgtgc ccagcgtctt gatcacgcgt
     5341 ggcggcggct gcgtcggttg gctcatgccg ccatcctagc gaatcacccg cgtccgttgt
     5401 gttacgaacg gcgcctgacc gacgagatca cgatcgatgc gctgacgagc ggctggacca
     5461 tcgtgcagcg caagaagggt catcgccact cgacggatga cctactcacc ggttggtatg
     5521 ccggcgaaat cgcgccggcc gcgacgcgcc tgctcgatct cggtgcgggt atcggcggcg
     5581 tcggcctcat cgctctgtgg cgcgcgcccg cgagcgcaac gctcgtcgct atcgaagcgc
     5641 aggatgtctc gttcggcctg ctccagcaga acatcgcgcg caatggcctc gacctgcgcg
     5701 tccgcgccat ccacggcgat ctgcgcggcg tgcgcttcca cggcctgttc gatctcgtga
     5761 ccggcagccc gccctactgg gacgtctcgc aaggcgtcgt ccccgccgac tcgcaaaagg
     5821 cgcacgcacg cttcgagctt cgcggcgacg tccgcgatta ctgcatcgca gcgcgcgccg
     5881 cactcgacga caacggtcgc ttcgtcttct gtttcccaac cgtgcaacgt gagcgcgccg
     5941 aagctgcagt cgccgccgcc gagctcgccg tcattcgctc gcgcgacgtc atcccacgcg
     6001 ccggcgccga gccgctgttc acgctcttcg catgccgccg cgcggaagat agcgacgagc
     6061 cgcacgttcg cgagcccgac cacgtcgtcc gcgacgagca cggcgagccg acgccgatgc
     6121 atcgagccgc ccgcgccgcg ctcggctttt agtgctacta aacggcgtgc ccgcccgcgt
     6181 cctcac
//
LOCUS       DATLBZ010000706         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6611457, whole genome shotgun sequence.
ACCESSION   DATLBZ010000706 DATLBZ010000000
VERSION     DATLBZ010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6611457"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VMZ53_34370"
     CDS             <1..207
                     /locus_tag="VMZ53_34370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33654.1"
                     /translation="YTYPATQWRMLFDSAASSNDELSSRTVHTLSSSRRSCQSQPRGS
                     QRTIACPCDEISRSYWAARALAAL"
     gene            801..1694
                     /locus_tag="VMZ53_34375"
     CDS             801..1694
                     /locus_tag="VMZ53_34375"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HUS33655.1"
                     /translation="MIAIPESPRMADVAELNERLAELRLCDATALDAIRRSLERHGQL
                     TPIVAFADGDHLETLDGFKRLRAARALGWSTVAITIADVVDGVAAKVQLAELHQRRGL
                     TEIEEAWLIRSLYRDDKLSQPEIARKLARHKSWVCRRLMLVEALDPVVQANVRVGLLA
                     PRAAIALAALPRGNQVAASGVVIQHGLTVRQTDQLVAALCQLPDDAARATWLAQRLEA
                     PASTPASRAPRSDADWLMIDIATLLRAGARLQARLLGAPLGALATPASELVLDGLGTL
                     SPVLAALARTIATMLDHERAA"
     gene            1691..>2272
                     /locus_tag="VMZ53_34380"
     CDS             1691..>2272
                     /locus_tag="VMZ53_34380"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS33656.1"
                     /translation="MTTWRSRDELVHQIVTLARSRMSKRAIARAVGVSRNTVKAVLAA
                     HAAQRSTTHSALPAPRTRAPRPKKTDAFRDRIAELFVRYPDITAQRVFEILTAEGFDG
                     GYTAIKKYVRTLRVPKKPAPSLQAPAYGPGEMAESDWSLYTIEFTSGTRLDVQAFSYV
                     LTYSPRKSYGIYTSNGLHALMDGHERAFARFKGC"
BASE COUNT          376 a          749 c          782 g          365 t
ORIGIN      
        1 tacacgtatc cggccacgca gtggcgcatg ctcttcgatt ccgcagctag ctccaacgac
       61 gagctcagct cgcgcacggt ccacacgctg tcgtcatcgc ggcgctcttg ccagtcccag
      121 ccgcgcggga gccagcgaac gatcgcgtgt ccgtgcgacg agatctcgcg ctcgtactgg
      181 gccgcgcgtg cgttagcggc gctctaagac ggatccactc ggcggcggct tcgtgagcca
      241 aggcgccgcg ttctccgcgg tcgcggcggc gctttcgtga cccaccctgg atgagtcgct
      301 gtgaccctga tggtcgatgg cgaagcggtc acgatgttcg gagtgccggg agcggttcac
      361 gccggcggtg acgacgggtg atgggcagaa gtgttgcgga ccgtcgtgcc gactcgcgag
      421 gcggcgaaag caagcacggg cgcgacgacg gcgtgacgtg gtcgagtttc gcgccgccga
      481 gcgagcgcgc caggaggtgc accgagaaag gcgcgacgca tccggatgtc acgcgccggc
      541 ttcgccgccg atcccattgg agttgcagga taaagtgcgt cggatcgtgg acgaggcgac
      601 gggcatgtca cgcgccggct tcgagcgcgg tgtgcgcgaa atcgtgaggg aactcggcca
      661 aaggtgggtc gctccggtcg gtggtcacgc gccgacttcg cccgagaagc caccgtgatc
      721 atgacggaat cgcgctcgaa ttgggcgcgt gtcacacgcc ggtatcgctc gtgatcgtgt
      781 cgcgtgctcg gatgacgccg atgattgcga tccctgaatc gccgcggatg gcagacgtcg
      841 ccgagctcaa cgagcgtctc gccgagctcc ggttgtgcga cgcgaccgcg cttgatgcaa
      901 tacgtcgctc gctcgagagg cacgggcagc tcacgccgat cgtcgcgttc gctgacggcg
      961 accacctcga gacgctcgac gggttcaagc gcctgcgtgc agcacgagcg ctcgggtggt
     1021 cgacggtcgc gatcacgatt gctgacgttg tcgacggcgt tgctgccaag gtgcagcttg
     1081 ccgagttgca tcagcgccgc gggctcaccg agatcgagga ggcatggctg atccgttcgc
     1141 tgtatcgcga cgacaagctc tcgcagcccg agatcgcgcg caagctcgct cgacacaaga
     1201 gctgggtctg ccgacgtctg atgctggtcg aggcgctcga cccggtcgtg caggccaatg
     1261 tccgcgtcgg tctgctcgcg ccgcgcgccg cgatcgcact tgcggcgttg ccgcgcggca
     1321 accaagtggc tgcctccgga gtcgtgatcc aacacgggct cacggttcgc cagactgacc
     1381 agctggtagc tgcgctgtgc cagcttcccg acgacgccgc gcgcgcgacg tggctcgcgc
     1441 agaggcttga agcaccggca tcgacgccgg cgtcacgtgc accgcgcagc gacgccgact
     1501 ggttgatgat cgacatcgcg acgctgctcc gcgcgggagc gcgtctccaa gcacggttgc
     1561 tcggcgcacc actcggcgcg ctcgcgacac cggcgagcga gctcgttctc gatggactcg
     1621 gcactctttc accggtactc gccgcgctag cacggacgat cgcgacgatg ctcgatcacg
     1681 agcgagcggc atgacgacgt ggcgctctcg cgacgagctc gtgcaccaga tcgtgacgct
     1741 ggcgcgcagc cgaatgtcga agcgagcgat cgcacgagcg gtcggcgtga gtcgaaacac
     1801 cgtcaaggcg gtgctcgccg ctcacgccgc gcagcgcagc acgacgcatt cggcgctgcc
     1861 ggcgccacgg accagggcac cacgcccgaa gaagacggac gctttccgcg accgaatcgc
     1921 agagctgttc gtccgatatc cggacatcac cgcccagcgc gtcttcgaga ttctgaccgc
     1981 ggaggggttc gacggcggct acacggcgat caagaagtac gtgcgcacgt tgcgcgtgcc
     2041 caagaagccg gcgccgagtc tccaagcacc ggcctatggc ccgggcgaga tggccgagag
     2101 cgactggtcg ctgtacacga tcgagttcac gagcgggacg cgcctcgacg tgcaggcgtt
     2161 ctcgtacgtg ctgacgtaca gcccgcgcaa gtcgtacggc atctacacct cgaatgggct
     2221 gcacgcgctc atggacggtc acgagcgtgc gttcgcccgc ttcaagggct gc
//
LOCUS       DATLBZ010000707         9431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6625051, whole genome shotgun sequence.
ACCESSION   DATLBZ010000707 DATLBZ010000000
VERSION     DATLBZ010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6625051"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1436)
                     /locus_tag="VMZ53_34385"
     CDS             complement(12..1436)
                     /locus_tag="VMZ53_34385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33657.1"
                     /translation="MGLATVGLLAGCPDRTISEVNPEQGRVEYKDIPVTVNRDIDILF
                     VIDDSPSMLDKQTNLKNNFPNFINVLNGIEGGLPNVHIGVTSSDLGTKGADGMTAPGI
                     GSGPGSCSQGGKNGNLTTNGSTLVMGNFISDTKNTDGTRNKNYTGTLEAAFSAMASVG
                     AGGCGFEQHIEAAKRALSNNPANAGFLRQNAYLAIIWIQDEDDCSMAHTTLLGSDTNT
                     LGPLQSFRCNRFGHACTGGGADSNAMNSVGTKTGCTSNDNSAYLTKVSDYITYFKGLK
                     SDPANVIVAGVMGPPETTNGMGMGENVELRTPPGGGQAIPAVSHSCTYQGANGPEVAD
                     PSIRMRQLFDAFPNRSTFNTICQQDLSGALTQIAQLLRTVIGSPCIEGNLADVDPNTA
                     GPQYDCSVSDVTNFGKANQQENVLPQCNNTGAPASSTNKPCWSIQADPTNCMSGSHLT
                     LKIERSEAPPADTHVISYCVTEGT"
     gene            complement(1637..2611)
                     /locus_tag="VMZ53_34390"
     CDS             complement(1637..2611)
                     /locus_tag="VMZ53_34390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33658.1"
                     /translation="MRHALIAITLLVGCTDEPAPSGPTATSHIQVADYLEHAPPLLDV
                     LIVVDNTQAMAPYQAKLPRLAADLSTAIHSLARDMADIRIAATTNGGALSPVLSDHSD
                     WFGVHSPSFTGSLESALTPLVDVGATASGPSQPLAAMRTALESSPAFLRPRASLVIVT
                     MSATDDASPSFDYVSFAKAAKSDPANVMVIGIYPPGSTNLDAFHHAFPNRNLVTSIDA
                     DDYAPAFADLPRLHRTTLEGSCMYQPADVDLEAPGAQIDCVAMAYDDGAEIARVPMCE
                     GGPPTTGSCWELVAGATSGCSDGSLAFELRGLWTWSLRPAIQLQCVVP"
     gene            complement(2596..3495)
                     /locus_tag="VMZ53_34395"
     CDS             complement(2596..3495)
                     /locus_tag="VMZ53_34395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33659.1"
                     /translation="MTACVESSSVPPGESHSIQFDWPARGVPQLDLLFVVDDTPAMAP
                     YRERILELGPLTASSLRGYSGGMPDVHIAVATETTLSTISDVHNNDGSRTTSYRGVLD
                     DVLASMLDVGAASSSPSQPLATIERALSRTDFVRPDTNFMTVTISANDDHSLDSVDHY
                     VDAVKSRTQNSVAAGVFPAQSPRLEHFHEAFDPFHAGFVTTLDDSDWSVVMEPTNSIV
                     DFLYPCFYDERPVEPYECSISMIENDVETVLPPCSSTPEGACWQIVPDPSCVGDRGIG
                     TTIDVRGYARPYRPHLVGQCVTH"
     gene            3643..4959
                     /locus_tag="VMZ53_34400"
     CDS             3643..4959
                     /locus_tag="VMZ53_34400"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS33660.1"
                     /translation="MKQRVLLLGPDDEARRALHMLVAKRGYDVAAATDLDSAKKHVAS
                     TECDVIVASAELAAGACALDGAPPVIALVRSRDHEAALALLDKGVRDVISEPIDDLAI
                     ALALRNISVVRKANGSNGTNGKALEVPDMIGDSPAMQKLAATIRLVAAQRTTVLILGE
                     SGTGKELVARSIHGLSPRRTKRFVAINCAAIPGTLLESELFGHVKGAFTDAVRDKPGL
                     FEDADGGTLFLDEVGEMPLALQAKLLRALQESEIRRVGDSASIKVDVRLIAATLRDLT
                     DDVAEGRFREDLFYRLNVLPVQVPALRDRPEDIPQLARFFALRHGERHHRSLELSDDL
                     LEALARQPWPGNVRELENVIERALVLADGPEINADFLATVMNVRGASGGVDDQELSIK
                     KATRSLEAELIRRALGVTQGNRTNAAKLLEISHRALLYKMKEYGIS"
     gene            5040..6086
                     /gene="pilM"
                     /locus_tag="VMZ53_34405"
     CDS             5040..6086
                     /gene="pilM"
                     /locus_tag="VMZ53_34405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HUS33661.1"
                     /translation="MAKQCIGLDIGSSAVKAVQLKKKGSGWALQAFGMQPLVPQTIVD
                     GTIMDQGAVSDAVRQLFGRLKLKAKDVAIAIAGHSVIIKKITVPMMKPDELAANIRNE
                     AEHHIPFGRDDVEIDYHVTNPMTPSGQTELLLVAAKKEVVSDYIQVVRDAGLSPQVVD
                     VAAFASQNGFEANYPLNPQETVVLINIGAAISNINIVRSGVSLFTRDVTIGGNAFTEE
                     IQKQMGIAADEAEAYKVGGTQTEDGVVPQEVIRVMEGVSEVMAGEFQRSLDFFLATTA
                     DANVTRIMLAGGSSKVASLHRAIERRSRLPLEVVDAWKRVEIDPTLDRTYLAAHSPEA
                     LIGVGLSMRAAGDK"
     gene            6089..6772
                     /locus_tag="VMZ53_34410"
     CDS             6089..6772
                     /locus_tag="VMZ53_34410"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HUS33662.1"
                     /translation="MIRINLLPHKRAKGRFAGASASEPSTRDILIGTGALVGAALLLF
                     ILVDQPKRSRLHDLEESNEQLQQQINDKNEQLKGKGNDDPGYETLKKLVEESDKRAQS
                     IIRLMSAKVVPANVLHELSEIMTTSHPPTMTDEMAKKVARDPNKRFDVAWDPTKVWLM
                     SFSDKADDGTFKLEGGAQAEADIVQLSKRLQASAYFDRVSLQTEERVSDRDTGITYYR
                     FVITGKVAY"
     gene            6781..7596
                     /gene="pilO"
                     /locus_tag="VMZ53_34415"
     CDS             6781..7596
                     /gene="pilO"
                     /locus_tag="VMZ53_34415"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HUS33663.1"
                     /translation="MADFARMPTRQKVMLFGIIGVVLGLIYYQFGYKKLKTRLAEAEA
                     EHSAKLALNSKLEKDIPEFAKLKARMTNLKRMIDENQKALPTEAELPAFFDMLNRKVL
                     ESGVEVVKAKQLKEVPVESFAKVPVEYEIQGSFLQIKKFFASLLPRKKKPGDPPATGE
                     SGDVEEKERIVSIENLSLSNPVVRNREIKLTARFVASTYRQEEKVDPKAKPGAAPATQ
                     GTNPSPAAAGTPKGAKDQTEKAIDKGDQRNQGATGVDEAKTPQGSATMKGGVP"
     gene            7593..8390
                     /locus_tag="VMZ53_34420"
     CDS             7593..8390
                     /locus_tag="VMZ53_34420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33664.1"
                     /translation="MKSALLAVVLVFTAACGDDPPPPPAPAAPPGAKAGAKPGGAKGK
                     QVLLQARQHVEEKVTCPPPEKATGPACNKDAPVCDAGKTCLQVGNGFNCEPCAERDSI
                     RHEFKDRDFVPDQTRDPFQSFIIVQKGLEEPPEKHEDLGPCRRQDQLVASNYSYLDMR
                     LVGIVAQGTQRKVLMMDRGNLGHIIRRGDCVGKEKALVKDIGPGFVTFVTQPDENDKA
                     PNRAPVEHSIQLNPKGLQVAPQPTDLTQQPTAPVVAPPTDTAPVVQP"
     gene            8482..>9431
                     /locus_tag="VMZ53_34425"
     CDS             8482..>9431
                     /locus_tag="VMZ53_34425"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HUS33665.1"
                     /translation="MTPKAMLVALAVVASSTAAYANNEVRAVTFSEDAGTTLVHVRGE
                     QTPTFTVYKLERPSRVVIDMPQARLSEALRGHESAATLSPNTWAVSTIAAQQLDDGAV
                     RVIVTLARPGRYDVKTDGNEVVVMVMPRDPAPKTANPADLARARSESEQAKKEMQAAE
                     QARAVAEKSSEVAKVEAERLRKVAADQAARAEAAQRAVEEAKKSNTADLAKAKQLAQA
                     AKAEADAAKLASTTAQVQAERMRIAAEQARRDALAEAAAAKADAAQARDQMAKTQADA
                     AKTRDEMAKTQQMIAQSKAEAEAAKREAAAAKQQVAMAKT"
BASE COUNT         1857 a         2893 c         3115 g         1566 t
ORIGIN      
        1 gagagcgacg actaggtgcc ttcggtcacg cagtacgaga tgacgtgcgt gtcagccggc
       61 ggcgcttcgc tgcgctcgat cttcagcgtc aggtgcgagc cgctcatgca gttcgtcgga
      121 tccgcttgga tcgaccagca cggcttgttc gtcgacgagg ccggcgcgcc cgtgttgttg
      181 cactgcggga ggacgttctc ctgctggttg gccttgccga agttcgtgac gtccgagacc
      241 gagcagtcgt actgcgggcc agcggtgttc gggtcgacgt cggcgaggtt gccttcgatg
      301 cagggcgaac cgatgacggt gcggagcagc tgagcgatct gcgtcagtgc gccggagagg
      361 tcctgctggc agatcgtgtt gaacgtcgag cggttcggga atgcgtcgaa cagctggcgc
      421 atgcggatcg atggatctgc gacttccggt ccgttcgcac cttgatacgt gcacgagtga
      481 gaaacggcgg ggatcgcctg gccgccgcct ggaggcgtac ggagctcgac gttctcgccc
      541 atgcccatac cgttcgtcgt ctcgggcgga cccatgacgc ctgcgacgat gacgttcgcc
      601 gggtcagact tgaggccctt gaagtacgtg atgtagtccg agaccttcgt gaggtaagcg
      661 ctgttgtcgt tcgacgtgca gcccgtcttc gtaccgacgg agttcatcgc gttcgaatcc
      721 gcgccgccac ccgtgcacgc gtgaccgaag cggttgcagc ggaatgactg cagaggtccg
      781 agcgtgttcg tgtccgaacc gaggagcgtc gtgtgcgcca tcgagcagtc gtcttcgtcc
      841 tggatccaga tgatcgcgag gtacgcgttc tggcggagga agcctgcgtt ggcggggttg
      901 ttcgagagcg cgcgcttcgc agcctcgatg tgctgctcga aaccgcagcc gccggcgccg
      961 accgatgcca tcgcggagaa cgcggcttcg agcgtgccgg tgtagttctt gttgcgcgtg
     1021 ccgtccgtgt tcttcgtgtc gctgatgaag ttacccatca cgagcgtcga accgttcgtc
     1081 gtcaggttgc cgttcttgcc gccttgcgag caggagccgg gacccgagcc gatacccgga
     1141 gccgtcatgc cgtcggcgcc cttggtgccg aggtccgacg acgtcacgcc gatgtgaacg
     1201 ttcggaaggc cgccttcgat gccgttcaga acgttgatga agttcgggaa gttgttcttg
     1261 aggttcgtct gcttgtcgag catcgacggc gagtcgtcga tgacgaacaa gatgtcgatg
     1321 tcacggttga ccgtaacggg gatgtctttg tattcgacgc gaccttgctc ggggttgacc
     1381 tccgagatcg tgcggtcggg acatccagcg agcaggccga ctgtggccag ccccaaaagc
     1441 gaagtcgtag taatggcgtt gcgcatcgac gcacctccga gaatgctcgg ccagtctaga
     1501 gcaactccct agccagtttg aagtccgagc agtttcaaac gcttactttt cgtcacacac
     1561 gcggaaggtg ccaactctgt tggcgaaatc gatcggttgt tggcgcggaa tgccccgcga
     1621 ttccgatcac ttgccgctag ggaacgacgc attgcagctg gatggcgggg cggagggacc
     1681 aggtccagag gccgcggagc tcgaatgcga gtgacccgtc ggagcagccc gaagtcgcgc
     1741 cggcaacgag ctcccagcac gagccggtcg tcggaggccc gccttcgcac atcggaacgc
     1801 gcgcgatctc ggctccatcg tcgtacgcca tcgcgacgca atcgatctgg gcgccgggcg
     1861 cttcgagatc gacatcggcg ggttggtaca tgcacgagcc ttcgagtgtc gtgcgatgga
     1921 gcctcgggag atccgcgaac gcgggcgcgt agtcgtcggc atcgatcgat gtgacgagat
     1981 tgcgattggg aaacgcgtga tgaaatgcgt cgaggttcgt gctgcccggc ggatagatgc
     2041 cgatgaccat cacgttcgct ggatcgctct tcgctgcctt cgcgaacgac acgtagtcaa
     2101 acgatggcga tgcatcatcg gtagcgctca ttgtcacgat gacgagcgac gcgcgagggc
     2161 gcagaaaggc tggcgaggat tccagcgccg tgcgcatcgc tgctagtggt tgcgacgggc
     2221 ccgatgcagt agcgccgacg tcgacgagcg gcgtgagcgc actctcgaga gagccggtga
     2281 agctcggcga atgcacgccg aaccaatcgg agtggtcgga gagcaccggc gagagcgcgc
     2341 cgccgtttgt cgtcgcggcg attcttatgt ccgccatgtc acgcgcgagc gaatggatcg
     2401 cggtgctcag gtcggcggcg agccgcggca gcttcgcttg atacggcgcc atcgcctgcg
     2461 tgttgtcgac gacgatcagc acgtcgagga gcggcggcgc gtgctcgagg tagtcggcga
     2521 cctggatgtg actcgttgct gtcgggccgc tcggcgccgg ctcgtcggta cagccaacga
     2581 gaagagtgat tgcgattagt gcgtgacgca ttggcctacc aggtgtggac gatatggacg
     2641 cgcgtagccg cggacgtcga tcgtggtccc gatcccacga tcaccgacgc aacttggatc
     2701 gggaacgatc tgccagcacg cgccttcagg ggtagacgag cagggcggca acaccgtctc
     2761 gacgtcgttc tcgatcatgg agatcgagca ctcgtacggc tcgacggggc gctcgtcgta
     2821 gaagcaggga tacaagaagt cgacgatgct gttcgtcggc tccatcacga cggaccaatc
     2881 gctatcatcg agcgtcgtga cgaagccggc gtggaacgga tcgaacgctt cgtgaaagtg
     2941 ctcgaggcga ggcgactgcg cagggaacac gccggccgca acgctgtttt gcgtgcgcga
     3001 cttcaccgcg tcgacgtagt gatcgacgct gtcgagcgag tggtcatcgt tcgcgctgat
     3061 cgtcacggtc atgaagttgg tgtcgggccg gacgaaatcc gttctcgaaa gtgcgcgctc
     3121 gatcgtcgcg agcggctgcg acggtgacga cgaagcagct ccgacatcca gcatcgacgc
     3181 gaggacgtcg tcgagcacgc ccctgtagct cgtcgtgcgg gagccgtcgt tattgtgaac
     3241 atcgctgatc gtcgagagcg tcgtctcggt tgctacggca atatgcacgt cgggcatccc
     3301 gccggagtag ccgcgcagcg acgacgcagt cagcggcccg agctcgagaa ttcgctcgcg
     3361 gtacggcgcc atcgccggcg tgtcgtcgac gacgaacaat agatcgagct gcggaacacc
     3421 gcgcgccggc cagtcgaact ggatgctgtg actttcgccc ggtggcaccg acgatgattc
     3481 cacacaggcg gtcatcaaga cgaggacaag ggcgctccgc acatgccatg gcaagagcaa
     3541 ggcgctcgcc acgtcacagt ctgcgacttt cacgcggttg cgattcgcgt cacgccaacg
     3601 gtgttggcaa tttgccaacg acagcgtgct acgattttcg tcgtgaagca gcgggtgttg
     3661 ctgctggggc ctgacgacga ggctcgtcgc gcgttgcaca tgctcgtcgc aaagcgcggg
     3721 tatgacgtcg cggccgcgac ggatctcgac agcgcgaaga agcatgtggc gagcacggag
     3781 tgcgacgtga tcgtcgcgtc ggcggagctc gcagctggcg cctgtgcgct cgacggtgca
     3841 ccgcccgtga ttgccctcgt ccgctcgcga gatcacgaag ctgcgctcgc gctcctcgac
     3901 aaaggcgtgc gcgacgtcat cagcgagccg atcgacgatc tcgcgatcgc gctggcgctg
     3961 cgcaacatca gcgtcgttcg caaagcgaac ggcagcaacg gaacgaacgg caaggcgctc
     4021 gaagttccgg acatgatcgg cgacagccca gcgatgcaaa agctcgcggc gacgatccgc
     4081 ctcgtcgccg cgcagcgcac gacggtgctg atcctcggcg agagtggaac cggcaaggag
     4141 ctcgtcgcgc gctcgatcca cggcctctcg ccgcggcgca cgaagcggtt cgtcgcgatc
     4201 aactgcgcgg cgattccggg cacgctgctc gagagcgagc tgttcggcca cgtgaagggc
     4261 gcgttcaccg atgcggtgcg cgacaagcca ggcctcttcg aagatgccga tggcggaacg
     4321 ctgtttctcg acgaggtcgg cgagatgccg ctcgcgctgc aggccaagtt gcttcgcgcg
     4381 ctgcaagagt ccgagatccg tcgcgtcggc gacagcgcgt cgatcaaggt cgatgtgcgc
     4441 ctcattgccg cgacgcttcg cgacctcacc gatgacgtcg ccgagggccg cttccgcgag
     4501 gacctgttct atcgcctcaa cgtgttgccc gtgcaggtcc ctgcgctgcg cgatcgtccc
     4561 gaggacattc cgcaacttgc gaggttcttc gcgcttcgcc acggcgaacg ccatcaccgc
     4621 agcctcgagc tgtcggacga cctcctcgaa gcgctcgctc gccagccttg gcccggcaac
     4681 gtgcgcgagc tcgaaaacgt catcgagcgg gcactcgtgc tcgccgacgg acccgagatc
     4741 aacgccgact tcctcgcgac cgtgatgaat gtgcgtgggg cctccggcgg agtcgacgat
     4801 caggagctat ccatcaagaa ggccacacga tctctcgagg cggagctcat ccggcgagcg
     4861 ctcggagtca cccagggaaa ccgcacgaac gccgccaagc tcctcgagat cagccaccgc
     4921 gcgctgctct acaagatgaa ggagtacggc atttcctgac ttacggaagt gcagcgttcc
     4981 ggcgaagctt tttggagatc gagctgcggc atgacaaaat tttccgtcgc agaggttcca
     5041 tggcgaaaca atgcatcggg ctcgatatcg gttcttccgc agtgaaggcc gtgcagctca
     5101 agaaaaaagg ctcggggtgg gcgctgcaag catttggcat gcagccgctc gtcccgcaga
     5161 cgatcgtcga cggcacgatc atggatcaag gcgccgtcag cgatgctgtt cgccagctgt
     5221 tcgggcggct caagctcaaa gcgaaggacg tcgcgatcgc gatcgccggc cactccgtga
     5281 tcatcaagaa gatcacggtg ccgatgatga aacccgacga gctcgcggcg aacattcgca
     5341 acgaagccga gcatcacatt ccattcggac gtgatgacgt cgagatcgat tatcacgtca
     5401 cgaatccgat gacgccgagc ggccagacgg agctgttgct cgtcgccgcg aagaaagaag
     5461 tcgtttccga ctacatccaa gtcgtgcgcg acgctggcct gtctccgcag gtcgtcgacg
     5521 tcgccgcgtt cgccagccag aacggcttcg aggcgaacta cccgctgaat ccgcaagaga
     5581 ccgtcgttct catcaatatc ggtgcggcga tctcgaacat caacatcgtg cgcagcggcg
     5641 tgtcgctgtt cacgcgcgac gtgacgatcg gcggcaacgc gttcaccgaa gagattcaga
     5701 agcagatggg catcgccgcc gacgaggccg aagcgtacaa ggtcggcggc acgcagaccg
     5761 aggacggcgt cgttccgcag gaagtcatcc gcgtcatgga aggcgtctcg gaagtgatgg
     5821 ccggcgagtt ccaacgctcg ctcgacttct tcctcgcgac gaccgccgac gcgaacgtca
     5881 cgcgcatcat gctcgcgggc ggaagctcga aggttgcgtc gctgcatcgc gcgatcgagc
     5941 gtcgctcgcg tctgccgctc gaagtcgtcg atgcgtggaa gcgcgtcgag atcgatccga
     6001 cgctggaccg cacgtacttg gccgcgcatt cacccgaagc cctcatcggc gtcggactct
     6061 cgatgcgagc ggcgggagac aagtagcgat gatcaggatt aacctcctcc cgcacaaacg
     6121 agcgaagggt cgcttcgccg gagcgtcagc gtccgagccg agcacgcgcg atatcttgat
     6181 cggcaccggc gctctcgtcg gtgcggcgct gctcttgttc atcctcgtcg atcagccgaa
     6241 gcgatcgcgg cttcacgatc tcgaggaaag caacgaacag ctccagcagc agatcaacga
     6301 caagaacgag cagctgaagg gcaagggcaa tgatgatccg ggctacgaga cgctcaagaa
     6361 gctcgtagag gaatccgaca agcgggcgca gtcgatcatt cgtctgatgt ccgcgaaggt
     6421 cgtgcccgca aacgtgctgc acgagctctc cgagatcatg acgacgagcc atccgccgac
     6481 gatgaccgac gagatggcga agaaagtcgc aagggatccg aacaagcgct tcgacgtcgc
     6541 gtgggacccg acgaaagtct ggctgatgtc gttctccgac aaagctgatg acggcacgtt
     6601 caagctcgag ggcggcgcgc aagcggaggc cgatatcgtg cagctctcga agcgcctgca
     6661 ggcgtccgcc tacttcgatc gcgtctctct gcagacagaa gagcgcgtct ccgaccgcga
     6721 tacggggatc acgtactacc gattcgtgat caccggaaag gtggcctact aatggcggtc
     6781 atggccgatt tcgcccggat gccgacgagg cagaaggtga tgctgttcgg catcatcggc
     6841 gtcgtcctcg ggctcatcta ctaccagttc ggctacaaga agctgaagac acggctcgcc
     6901 gaggcggagg cggagcactc cgcgaagctc gcgctgaaca gcaagctaga gaaggacatc
     6961 cccgagttcg cgaagctcaa agcgcgaatg acgaacctca agcgcatgat cgacgagaac
     7021 cagaaggcgc tgccgaccga agccgagctg ccggcgttct tcgacatgct caaccgcaag
     7081 gtgctcgagt ccggcgttga agtcgtcaag gccaagcaac tgaaggaagt gccggtcgag
     7141 tcgttcgcga aggttccggt cgagtacgag atccaaggct cgttcctgca gatcaagaag
     7201 ttcttcgcgt cgctgctgcc gaggaagaag aagccgggcg atccgccggc gacgggcgag
     7261 tcaggtgacg tcgaagagaa ggagcgcatc gtctcgatcg agaacctgtc gctctcgaat
     7321 ccggtcgtcc gcaatcgcga gatcaagctg accgcgaggt tcgtcgcgtc gacgtatcgc
     7381 caggaagaga aggtcgaccc gaaggccaag ccgggcgctg cgccggcgac gcagggaacc
     7441 aatccgtcgc cagccgctgc gggcacgcca aagggcgcga aggatcagac agagaaggcg
     7501 atcgacaagg gtgatcagcg caaccaaggc gccaccggcg tcgacgaggc caagacaccg
     7561 caaggatccg cgacgatgaa gggaggcgtg ccatgaagag cgcactcctc gcagttgtcc
     7621 tcgtgttcac cgcggcgtgc ggtgacgatc cgccaccacc gcctgcacca gcggcaccgc
     7681 cgggcgcaaa ggccggagcc aagcctggcg gcgcgaaggg caagcaggtg ctgctgcaag
     7741 cgcgccagca tgtcgaagag aaggtgactt gtccgcctcc cgagaaggcc accgggcccg
     7801 cgtgcaacaa ggacgcgccc gtgtgcgacg caggcaagac ctgtctgcag gtcggcaacg
     7861 gtttcaattg tgagccgtgc gccgagcgcg actcgatccg gcacgagttc aaggatcgtg
     7921 acttcgttcc cgaccaaacc cgcgatccgt tccagtcgtt catcatcgtg cagaagggtc
     7981 tcgaggagcc gccggagaag cacgaggacc tcgggccctg ccgccggcaa gaccagctcg
     8041 tcgcctcgaa ctacagctat ctcgatatga ggctcgtcgg catcgtcgcg caggggacgc
     8101 agcgcaaggt gctcatgatg gaccgcggca acctgggcca catcattcgc cgcggcgact
     8161 gcgtcggcaa agagaaagcc ctggtcaagg acatcggtcc tggcttcgtc acgttcgtca
     8221 cgcagcccga cgagaacgac aaggcgccca accgagctcc tgtcgagcat tcgattcagc
     8281 tcaatccgaa gggcctgcag gtcgcgccgc agccgaccga cctcacccag cagcccaccg
     8341 cacccgttgt tgctccgccg accgacaccg ctccggtcgt gcagccctga tcgttcgact
     8401 tcgttaagcg aagccaagac gatcgaaaat ttgtcgcctc gcgcagagcg ggcgacaatt
     8461 ttttttggag tcgccatgat catgacgccg aaagcaatgc tcgtcgccct cgcggtggtc
     8521 gcgtcgagca ccgccgcata cgcgaacaac gaagtgcgtg cggtgacgtt ctccgaggac
     8581 gcaggcacga cactcgtgca cgttcgcgga gaacaaacac cgacgttcac cgtctacaag
     8641 ctcgagcgtc cgagtcgtgt cgtgatcgac atgccgcaag ctcgactgag cgaagcgttg
     8701 cgtggacacg agagtgccgc gacgctctcg ccaaacacgt gggcggtctc gacgatcgct
     8761 gcccagcagc tcgacgacgg cgccgttcgc gtcatcgtca cgctcgcacg gcctggacgt
     8821 tacgacgtca agaccgatgg caacgaggtc gtcgtcatgg tgatgccacg cgacccggcg
     8881 ccgaagacgg cgaacccggc agacctcgcg cgcgctcgat cggagagcga gcaagcgaag
     8941 aaagagatgc aggccgccga acaggcgcgc gctgttgcgg agaagtcgag cgaggtggcg
     9001 aaggtcgagg cagagcgcct tcgcaaggtc gccgccgacc aggctgcacg cgcagaagcc
     9061 gcgcagcgcg ctgtcgagga agccaagaag tcgaacaccg cggatctcgc gaaggcgaaa
     9121 cagctagcgc aagcggcgaa ggccgaagcc gatgcggcga agctggcgtc gacgaccgcg
     9181 caggtgcagg cggagcggat gcggatcgca gccgagcagg cgcgtcgcga tgcgctcgcc
     9241 gaggcagccg cagcaaaggc cgacgccgcg caagctcgcg accagatggc aaagacgcaa
     9301 gccgacgccg cgaagacccg cgatgagatg gcgaagaccc agcagatgat cgcgcagtcg
     9361 aaggccgagg ccgaggctgc gaagcgcgaa gcggccgccg cgaagcagca agtcgcgatg
     9421 gcgaagaccg a
//
LOCUS       DATLBZ010000708         7362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6629620, whole genome shotgun sequence.
ACCESSION   DATLBZ010000708 DATLBZ010000000
VERSION     DATLBZ010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6629620"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VMZ53_34430"
     CDS             complement(<1..805)
                     /locus_tag="VMZ53_34430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33666.1"
                     /translation="MTSSTTPIIAFSKDWHEDHTSNHHVLRELAKTRRVLWLNSLATR
                     KPNLGSTRDIGKIKRKLNEFSKGAVNVENDLWVATPLVLPLPQSAVAQQMNRQILRWT
                     IQALRRQLGIDRFQLWTFLPNTAPYVGTLGEDLAVYYCVDEWATFNGLDRAGTIAMER
                     QLLERVDVTFTTSLALAEKKKQFCPSTFLAPHGVDAAKFARALDEKLEVPSDLAALKG
                     PKIGFFGTLRDFLDYELMAHIAKARPDWQLALIGQKMCDIGPLEGLPNVH"
     gene            979..2862
                     /locus_tag="VMZ53_34435"
     CDS             979..2862
                     /locus_tag="VMZ53_34435"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HUS33667.1"
                     /translation="MASAERDLVGSVVLTRYRVIAKAASGATADIYEAEHVTSKSRVA
                     LKVLANNDASSDVAARFLREGKTLGMFRHPSIVELLEVGRLEDGRLFLATEMVRGSSL
                     RQLLARGAIEPRRALSITREVLDALGHAHGMGIIHRGMRPETVMVTDAGVKVLDFGVA
                     KLATDTAAVMGENKLTRTGIAVLGDPRYSAPEVARGGDTDARADLYAVGMMLKELLGA
                     AATKTPELQLLIADSTQMNRDKRYPSAAQMRDGVDQAIASLDELDVMAADDRAARAAA
                     PIAPSSGAVAEPMAAASSTSEGWSLDSQPVLAAPPQVSPPDAMGAPPARPSASFLPGV
                     TERPSKPSVPPIAPIADPPSAPYVGSTPPQTVSADPLFTATPQVPTAPVTHTGPWHGS
                     ASLTASEASRSPSSWAGPKGKRSFADLVAVAKKHRVIVGGVLGALVLVIVLVALFSGG
                     SSKKKVTPGQDGKALLARGRAELEAGHRADAVSLYERAIAADSGLAKSDDIRHDAQAI
                     VESKDAVAAVLALELLASRVSPPAKDVILATAAAGKNGDVRRRALAIAERDGFADEID
                     RVASLSLDLTQATTCDDRRAVIAKLRATADKRAVAPLKKAKVYKCVERDATDAIAELE
                     AQP"
     gene            2859..4217
                     /locus_tag="VMZ53_34440"
     CDS             2859..4217
                     /locus_tag="VMZ53_34440"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HUS33668.1"
                     /translation="MTSDEKQRVLALLMKHGWNSTSFQILEPGFEYWFDGDDACVGYV
                     DTGGAWVAAGPPIAAAQRIADVAERFSAAARENGRRVCCFGTEVRFSDIAQWPSIRIG
                     EQPLWIPAEWDATIKSSKSLREQLRRARAKGVVVDELTPAQLAAGQPMRDELDRLVGS
                     WQKSQALAPMGFLVKVELFAFPQERRYFVARRAGEIVAFLGVIPIYARRGWFFEDFIR
                     GPAAPNGTVELLIDAGMRAAKAGTIEIVTLGLAPLAGDVSPLLRFARRWGRLLYDFDG
                     LRAFKAKLKPKSWDPIFLSYPSGGNPVLATKDTLTAFSRGGLLRFGIETMLRGPTVVV
                     QALALLLAVWTVLLALPIATRWFPSPAWQWGWVAFDVAIFVALFALSRKWRHRLATLV
                     AAAITADAVTTLGEALFYNVPHRRGPLDFVVIAIAIAAPTFASIVLWNARARAATGAA
                     RA"
     gene            complement(4237..4473)
                     /locus_tag="VMZ53_34445"
     CDS             complement(4237..4473)
                     /locus_tag="VMZ53_34445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33669.1"
                     /translation="MSPLRPDREKADTMFVGNPLDMIEVLESSRETISTVVLTGRYAA
                     NRELTAYLTQTYPALSIVAGRAGEEPDPYLPTFS"
     gene            4812..6335
                     /locus_tag="VMZ53_34450"
     CDS             4812..6335
                     /locus_tag="VMZ53_34450"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HUS33670.1"
                     /translation="MRNLFVGLVLGSSLLAACASDSPVDEEISNVSAAKSRYVVVFRS
                     DTLPGDAASRITKAGGKAGKLLSSVGVATAIGDATFASKLAKDASVLSVGKEHSYFAP
                     KTQMKKLDLAGQEGPLGLLYFLQWDIRRVGAPAVWARTAPGAARPRVAVLDTGVMDDH
                     PDLAGQVGAMRSFSYCSTSGGPSNSPAYPKYSTYIDVVDVGGEWMWDGTCNPISEVIG
                     VPAMYEPHGTHVSGTIAGTANVGVIGVAPNAEIEAYKVFDRVLLPTGEFVLNGFEGPI
                     FDAIVDASNTGYPVISMSLGGTFDKRDGRASYLAWQRVLQYANKKGTLVVAAAGNSGL
                     DLNGTIVNVPSDVPTVLNVSATGTNALDWNADGQLVSVGSDVLSFYSNFGAPVDVSAP
                     GGDFGPAFNVNDPSTWGNQWVEHMILSSYIFEFDTETVDLDNRPVLAPAGSAGWAFFM
                     GTSMATPHVAAVAAQVRALHPTWTPGAVRSWLKDHAEAIGPRQQFGHGLVNADLAVH"
     gene            complement(6329..6805)
                     /locus_tag="VMZ53_34455"
     CDS             complement(6329..6805)
                     /locus_tag="VMZ53_34455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33671.1"
                     /translation="MRLFLVAILVACADPTPAPAPAPAPPPTTIAPPVPERVMARQTV
                     AWSQVESTAGCFFFSGPDGTDNRLDGTATIDRDGTHVTMRIGSALFEGTYREGTLDLM
                     RISSHSFDGPWLALERMHGVYARGTMTARYRYNECELAGECPGRCTLTGTITLTPQ"
     gene            6895..>7362
                     /locus_tag="VMZ53_34460"
     CDS             6895..>7362
                     /locus_tag="VMZ53_34460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33672.1"
                     /translation="MGFCALALAGASANAEGPARKAARTAPSTVKPRSPQKPTKVAAK
                     APVTPAPIVAAATPIEAAPVPLVAPVAVGAPESAPPVPVEVLIGDDTAPLLALDQKPM
                     TEAELVVEYQRVGRDIAKLQKDRGTDKVLDLWPTFRAIKLKESCKTAETRLNLE"
BASE COUNT         1220 a         2405 c         2469 g         1268 t
ORIGIN      
        1 gatggacgtt cggcagaccc tcgagcgggc cgatgtcgca catcttctgg ccgatcagtg
       61 cgagctgcca atcagggcgc gccttcgcga tgtgcgccat cagctcgtag tcgaggaagt
      121 cgcggagcgt gccgaagaac ccgatcttgg ggcccttgag cgccgcgaga tccgagggca
      181 cttctagctt ctcgtcgagc gcgcgcgcga acttcgctgc atccacgccg tgcggcgcga
      241 ggaacgtcga cgggcagaac tgcttcttct tctccgcgag cgcgaggctc gtcgtgaacg
      301 tcacgtcgac gcgctcgagc agctggcgct ccatcgcaat cgtacccgcg cgatcgagac
      361 cgttgaacgt ggcccactcg tcaacgcagt agtaaaccgc gaggtcttcg ccgagcgtgc
      421 cgacgtacgg cgcggtgttc ggcaagaacg tccagagctg gaagcggtcg atgccgagct
      481 gacggcgcag cgcctggatc gtccagcgca ggatctggcg gttcatctgc tgcgcaactg
      541 cgctctgcgg cagcggcagc acgagcggcg tcgcgaccca cagatcattt tcgacattga
      601 cggcgccctt ggagaactcg ttcagcttgc gcttgatctt gccgatgtcg cgcgtcgagc
      661 cgaggttcgg cttgcgcgtg gcgagagagt tcagccacag cacgcggcgc gttttcgcga
      721 gctcgcgcag cacgtgatga ttcgacgtgt ggtcttcatg ccagtctttc gagaaggcga
      781 tgatgggcgt cgtggaggag gtcatgccgt cagcttgtct ccacatcgcg tgccgaccac
      841 gagaacccct ccgagcaacg gtgagaattg cgtaatcgat acgccgatgt gcgcccacgc
      901 gcgcgcgctc accgaaaacc tcgtaaggcg ttttccctaa gcacgtgacc cagagccacg
      961 tcgtgtaaat tcgactccat ggccagcgcc gaacgcgatt tggtcggcag cgtggtgctg
     1021 acgcgctacc gcgtcatcgc aaaagccgcg agtggcgcga ctgccgatat ttacgaagcc
     1081 gagcacgtca cgtcgaagag ccgcgtcgcg ctgaaggtcc tcgcgaacaa cgacgcgagc
     1141 tccgacgtcg cagcgcgctt cctgcgtgaa ggcaaaacgc tcggcatgtt ccgccacccg
     1201 agcatcgtcg agctcctcga ggtgggccgc ctcgaagacg gtcgcctgtt cctcgccaca
     1261 gagatggtgc gcggctcgtc gcttcgtcag ctgctcgcac gcggcgcgat cgagccacgc
     1321 cgcgcgctct cgatcacgcg cgaggtcctc gacgcgctgg gccacgcgca cggcatgggc
     1381 atcatccatc gcggcatgcg gccggaaacc gtgatggtca cggacgcggg cgtcaaagtg
     1441 ctcgacttcg gcgtcgcaaa gctcgcgacg gacaccgccg cggtcatggg cgagaacaag
     1501 ctcacgcgca ccggtatcgc ggtgctcggc gatccacgct actcggcgcc cgaagtcgca
     1561 cgcggtggcg acaccgatgc gcgcgccgat ctctatgcgg tcggcatgat gctcaaagag
     1621 ctgctcggcg ctgccgcgac gaagacgcca gagctccagc tcctcatcgc ggacagcacg
     1681 cagatgaacc gcgacaagcg ctacccgtct gccgcgcaaa tgcgagacgg cgttgaccaa
     1741 gcgatcgcgt ccctcgacga gctcgacgtg atggccgccg acgatcgcgc cgcgcgcgcg
     1801 gcagcgccga tcgcaccatc atcgggtgcc gtggcggagc cgatggcggc ggcgagctcg
     1861 acgtcggaag gctggtcgct cgattcgcaa cctgtcctcg cggctccgcc gcaggtgtcg
     1921 cctcctgacg cgatgggcgc gccgccggcg aggccctcgg cgtcgttctt gcccggtgtg
     1981 accgagcgac cgtcgaagcc gagcgtcccg ccgatcgcgc cgatcgcaga cccaccttcg
     2041 gcgccgtacg tcggctcgac gcctccgcag accgtctcgg cggatccgct gttcacggca
     2101 acgccgcagg ttccgaccgc acctgtgacg cacaccgggc cctggcatgg gtcggcgagc
     2161 ctcaccgcgt cagaggcatc gcgatcacct tcgtcgtggg ctggtccgaa aggcaaacgc
     2221 tcgttcgccg atctcgtggc ggtcgcaaag aagcaccgcg tgatcgtcgg cggcgtgctc
     2281 ggcgcactcg tcctcgtgat cgtgctcgtc gcgctcttct cgggtgggtc gtcgaagaag
     2341 aaggtcacac ccggacaaga cggcaaggcg ttgctggcgc gcggccgcgc ggagctcgaa
     2401 gcgggacatc gcgccgatgc ggtgtcgttg tacgagcgcg ccattgccgc cgatagcggg
     2461 ctcgcgaaga gcgacgacat tcgccacgac gcgcaggcga ttgtcgagag caaggacgcg
     2521 gtcgccgccg tgctcgcact cgagctgctc gcatcgcggg tgtcgccgcc ggcgaaagac
     2581 gtgatcctcg cgaccgctgc ggccggcaag aacggcgatg ttcgccggcg cgcgctcgcg
     2641 atcgccgagc gggacggctt tgccgacgag atcgatcgcg tggcgagcct atcgctcgat
     2701 ctcacgcagg cgacgacctg cgacgaccga cgcgcggtga tcgcgaagct gcgcgcgacg
     2761 gccgacaagc gcgcggtggc gccgctcaag aaggcgaagg tctacaagtg tgtcgagcgt
     2821 gatgcgactg acgcgatcgc cgagctcgaa gcgcagccat gacgagcgac gagaagcagc
     2881 gcgtgctcgc gttgctcatg aagcacggct ggaactcgac gtcgtttcag attctcgagc
     2941 cgggcttcga atactggttc gatggcgatg acgcgtgtgt cggctatgtc gatacgggcg
     3001 gcgcgtgggt cgccgcgggc ccaccgattg cggcggcgca gcgaatcgcc gacgtcgcgg
     3061 agcggttctc cgcagcggcg cgcgagaacg gacgacgcgt gtgttgcttt ggtacggagg
     3121 tccggttttc ggacattgcg cagtggccaa gcattcgcat cggcgagcag ccgctgtgga
     3181 ttcccgcgga gtgggacgcg acgatcaaat cgagcaagag cctccgcgag cagctccggc
     3241 gcgcacgcgc caagggcgtc gtcgtcgacg agctcacccc ggcgcagctc gccgcggggc
     3301 agccgatgcg cgacgagctc gaccggctcg tagggagttg gcagaagtcg caggcactcg
     3361 cgccgatggg atttctcgtc aaggtagagc tgttcgcgtt tccccaggag cggcgctact
     3421 tcgtcgcgag gcgcgcaggc gagatcgtcg cgttcctcgg cgtgatcccg atctatgcgc
     3481 gccgcggttg gttcttcgag gacttcatcc gcgggccggc tgcgccgaac gggacggtcg
     3541 agctcttgat cgatgcgggc atgcgcgctg cgaaagccgg cacgatcgag atcgtgacgc
     3601 tcggtctcgc gccactcgcg ggagacgtct caccgctgtt gcggttcgcg cggcggtggg
     3661 gccggctgct ctacgacttc gatggtctgc gcgcgttcaa ggccaagctc aagccgaaga
     3721 gctgggatcc gatcttcttg tcgtatccga gcggcggcaa cccggtgctc gcgacgaaag
     3781 acacgctgac ggcgttctcc cgcggtgggc tgttgcgctt cgggatcgaa acgatgctgc
     3841 gtggcccgac ggtggtcgtc caggcgctcg cgctgttgct cgccgtgtgg acggtgctgc
     3901 tcgcgctacc gatcgcgacg cggtggttcc catcgccggc gtggcaatgg ggctgggtcg
     3961 cgttcgacgt cgcgatcttc gtggcgctgt tcgcgttgtc gcggaagtgg cggcatcggc
     4021 tcgcgacgct cgttgccgct gcgatcaccg cggacgcggt cacgacgctc ggcgaggcac
     4081 tcttctataa cgtcccgcat cggcgcgggc cgctcgactt cgtcgtcatc gcgatcgcga
     4141 tcgcggctcc gacgttcgcg tccatcgtgc tgtggaatgc gagagcccgc gcggcaacgg
     4201 gtgcggcgcg ggcttgagga cggaaaaacc gaaggctcag gagaacgtcg gcaggtacgg
     4261 gtccggctct tcgccggcgc ggcccgcaac gatcgacagc gcggggtacg tctgcgtcag
     4321 gtacgcggtc agctcccggt tcgcggcgta gcgtccggtc aagacgaccg tcgagatcgt
     4381 ctcgcggctg ctctccagca cttcgatcat gtcgagtgga ttgccgacga acatggtgtc
     4441 cgccttctcg cggtcaggcc gcagcggcga cacgaccaac acacgattcc ctttcgatct
     4501 gcattcgctc atagcgggcc ttgcagcctg cacgtttgga accacgctgg ggtgcgttca
     4561 cgccgaggaa gccaagcgag caagcaggct tgcgtccagt gatccggctt cgagctccgc
     4621 tgaatcgcgg cgaaaaaatc gtgcgcggag gcgcatcgag cgatggcgca cgcgcactcc
     4681 cgcgccggct actcggctag ccaccgcgcc aaaaagacgc gagcaagatc acaaaccgtt
     4741 cgacttacct tcctctcaca gcggcctccc ggttgcacaa gcgcctccgt tcaacctgag
     4801 tggagtgtct cgtgaggaat ttgtttgttg gtctggtcct gggttcgagc ttgctcgctg
     4861 catgcgcgag tgattcgccg gtcgatgagg agatctccaa cgtcagtgcc gcgaagtcgc
     4921 gctacgtggt cgtcttccgc agcgataccc ttccgggtga tgcggcgtct cgcatcacga
     4981 aggccggcgg caaggctgga aagcttctga gctccgtcgg tgtcgccacg gcgatcggcg
     5041 atgcgacgtt cgcgtcgaag ctcgcgaagg acgcctcggt tctctcggtc ggcaaagagc
     5101 actcgtactt tgcgccgaag acgcagatga agaagctcga tctagcgggt caagaaggac
     5161 cgctcggtct cctctacttc ctgcaatggg acatccgccg cgtcggcgcg cctgccgtct
     5221 gggctcgtac tgcgcccggt gcagcgcgtc cgcgcgtcgc cgtcctcgat acgggagtca
     5281 tggacgatca cccggatctc gccggccagg tcggcgcgat gcgctcgttc tcgtactgca
     5341 gcacgtcggg tggcccgagt aactcgcccg cgtacccgaa gtactcgacg tacatcgatg
     5401 tcgtcgacgt cggtggcgag tggatgtggg acggcacgtg caatccgatt tccgaggtca
     5461 ttggcgttcc cgccatgtac gagccgcacg gcacgcacgt gtccggaacg atcgcgggca
     5521 ccgcgaacgt cggcgtcatc ggcgtggcgc cgaacgcaga gatcgaggcg tacaaggtct
     5581 tcgaccgagt gctcctgccg accggcgagt ttgtcctcaa cggcttcgaa ggcccgatct
     5641 tcgacgcgat cgtcgacgct tcgaacaccg gctacccggt gatcagcatg agcctcggcg
     5701 gcacgttcga taagcgcgac ggccgtgcga gctatctcgc gtggcagcgc gtcctgcagt
     5761 acgcgaacaa gaagggcacg ctcgtcgtcg cggccgccgg caacagcggt ctcgatctca
     5821 acggtacgat cgtcaacgtt ccgtccgacg tcccgacggt gctcaacgtc tcggcaaccg
     5881 gcacgaacgc actcgactgg aacgcagacg gtcagctcgt ctccgtcggt tccgacgtgc
     5941 tgtcgttcta ctcgaacttc ggcgcacccg tcgatgtgtc ggcaccgggc ggtgacttcg
     6001 gcccggcgtt caacgtcaat gacccgagca cctggggcaa tcagtgggtc gagcacatga
     6061 tccttagctc gtacatcttc gagttcgata cggagaccgt cgatctcgac aatcgcccgg
     6121 tgttggcgcc agcgggctca gccggctggg cgttcttcat gggcacgtcg atggcgacgc
     6181 cgcacgtcgc tgcagttgcc gcgcaggttc gcgcgctcca tccgacgtgg acgccgggcg
     6241 cggttcgcag ctggctcaaa gatcacgccg aagcgatcgg tcctcggcag cagttcggtc
     6301 acggtctcgt caacgcagac ctcgccgttc actgaggcgt gagcgtgatc gtgcccgtga
     6361 gcgtacagcg ccccgggcat tctcccgcga gctcgcactc gttgtagcgg tagcgagcgg
     6421 tcatcgtgcc gcgcgcgtag acgccgtgca tgcgctcgag cgcgagccac ggtccgtcga
     6481 acgagtgcga gctgatcctc atcagatcga gcgtgccctc gcgataggtg ccttcgaaca
     6541 gcgcgctgcc gatcctcatc gtcacgtgcg tgccgtcgcg atcgatcgtc gcggtgccgt
     6601 cgagccggtt gtcggttccg tcggggccgc tgaagaaaaa gcagccggcc gtgctctcga
     6661 cctggctcca ggcaaccgtc tgtcgcgcca tcactcgctc gggtaccggc ggtgcgatcg
     6721 tcgtcggtgg cggcgcggga gcaggtgccg gcgctggcgt cggatccgca caagcaacga
     6781 ggatcgcaac gagaaacagc cgcaccgtta ccccaatcct accttgtaat ctttatccga
     6841 catggctagt gtccgtcgta agtgtccagg tcgacgggtt catataggtg gctagtgggg
     6901 ttctgcgcgc tcgctctcgc cggagcaagt gcaaatgcag agggaccggc acgcaaagcg
     6961 gcgcgcactg caccttcgac ggtgaagccg cgcagcccgc aaaagccaac gaaggtcgcg
     7021 gcgaaagcgc cggtgacacc tgcgccgatc gtagctgcgg ccacgccgat cgaagctgca
     7081 ccggtgccgc tcgtcgcgcc ggtcgccgtc ggagcgccag aatccgcacc gccggttccc
     7141 gtcgaggttc tgatcggcga tgacaccgcg ccgctgctcg cgctcgatca gaagccgatg
     7201 acggaagccg agctcgtcgt cgagtatcag cgcgtcggtc gcgatatcgc gaagctgcag
     7261 aaggatcgcg gcaccgacaa ggttctcgat ctgtggccga cgtttcgcgc gatcaagctc
     7321 aaggagtcgt gcaagaccgc cgagacgcgc ctcaacctcg ag
//
LOCUS       DATLBZ010000709         3608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6669307, whole genome shotgun sequence.
ACCESSION   DATLBZ010000709 DATLBZ010000000
VERSION     DATLBZ010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6669307"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..2622)
                     /locus_tag="VMZ53_34465"
     CDS             complement(88..2622)
                     /locus_tag="VMZ53_34465"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HUS33673.1"
                     /translation="MLQVAIAQFAGATVLHVPPLAAGLLASTIRRAAVAAEVRVHVRR
                     QPVEVAAAALANADVVGLSLYTWHARYALEVARRAKLLRKDLVIVAGGPSVPRRDATA
                     FLDAHPWLDALVLGEGELAFLDIIERAQRGADFTAIAGVVTRTAATISVGPPRPRLAT
                     FDAIGSPYLDGTFDELRARDEIPAMAAVVLETNRGCPFACTFCDWGQATQSRVNELPL
                     ERIERELAWIGERKISYLYLVDANFGIRPRDVEITKAIGRIANTHDAPRFVFFHLTKN
                     ATQKNLRTVEILREHGVRTQVALSMQDFDADVLTAIRRDNIKPEHALALREHCHALGL
                     STVNELMLGLPAQTAASIRRSLVAALTPSASDSFFVYPTRVLENAEMADPAYRARFAI
                     ETRQVPSWPADPADEMHVVEREELIVATSSLPVEDWRRAYAFGHLLSAAWNQRLLQTT
                     VHLLAFVLEADVAQLVDGLLDAHAQLRGELDRYTQAILDETATVLAIEGWGNRRYEPA
                     DAVCACAFADPDRFYASAAAVATRIAGPLAAEAVAWDALRFSTRARTLRFEHDWLGYE
                     AAMADRPRPAARPMIVSVTPAPAQAAGNFALEARKHARASVTRIGEPFVPTTRDTLGA
                     RAREDGFAYLPRALSAATLAPLRTLVDQELVQRGWLVDGHSDPALRLGRWDDARWHAF
                     LATIMASHEYKELATHPELLALLRAVLDAEPTPCAGDVCRLVSPNALDLTTPPHQDAA
                     YLTPTLWTAWLPLRACPLELGPLAVLPGSHRDGVRPHAPLGSDGVIGITTSLDDARWQ
                     TSDLEEGDVILFSALTVHRALPNVTANRLRVSVDFRYEPTARSPRS"
     gene            2698..>3608
                     /locus_tag="VMZ53_34470"
     CDS             2698..>3608
                     /locus_tag="VMZ53_34470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33674.1"
                     /translation="MRARREWVTRALVVAGVVVLGVLFSLAYGFELGTGNQHTYLLDP
                     LVRADPTLYRQDWFVTQNHHYHFAFATVTAPLFSLDPDGARAFGIAQILISVATFGAI
                     YGLVAAATERGRFTIFAGLCGLILLGGDRALGGSYLYAGYLQPSSFSVLAWLLALNAW
                     LRNRSALAGIALGIGAVFHLNYALLGIGVFGLAALATHPRDWRRIAMLLAPSLIVVIA
                     FLPTMLAASKTSDDALALRVLVKYLFPGHFKPVQLWLELASLLGWFVLLLAIKTEDRS
                     DSSSRVFNFGVATMGACILAVVVVSIP"
BASE COUNT          567 a         1205 c         1226 g          610 t
ORIGIN      
        1 ggcggcagcg ttacaacgat ggcagcggct gggatccgat ggaggatcgc gggagcatca
       61 cgcagaacca ctacgaccac gttcacgtca gcttcgagga gaccgcgccg taggctcgta
      121 gcggaagtcg accgagacgc gcaggcgatt ggccgtcacg ttcggcagcg cgcggtgcac
      181 ggtcagtgcg gagaacagga tcacgtcgcc ctcctcgaga tcgcttgtct gccagcgcgc
      241 gtcgtcgagc gatgtcgtga tgccgatgac gccgtcggag ccaagaggcg cgtgcgggcg
      301 cacgccgtcg cgatgcgagc ccggcaacac ggcgagcggg ccgagctcga gcggacacgc
      361 gcgaagtgga agccatgcgg tccacagcgt cggcgtcaga tacgcagcgt cctgatgcgg
      421 cggcgtcgtc agatcgagcg cgttgggcga aacaagacga cacacgtcgc ctgcgcatgg
      481 cgtcggttcg gcgtcgagca cagcgcgcag cagcgcgagc agctcgggat gcgtcgcgag
      541 ctccttgtat tcgtgggacg ccatgatcgt cgcgaggaat gcgtgccacc gcgcgtcgtc
      601 ccagcggccg aggcgcagcg ctgggtcgct gtgtccgtcg acgagccagc cccgttgcac
      661 gagctcttga tcgacaagcg tgcgaagcgg tgcgagcgtc gcggcggaca gcgcgcgggg
      721 cagatatgca aagccgtctt cgcgcgcgcg agcaccgaga gtgtcgcgag tagtgggcac
      781 gaacggctcg ccgatacgag tcaccgacgc gcgcgcgtgc ttgcgcgctt ctaatgcgaa
      841 gtttccggcc gcttgcgcag gcgcgggcgt cacgctgacg atcatgggac gcgcagcggg
      901 tcgcgggcga tcggccatcg ctgcttcgta gccgagccag tcgtgttcga atcgcagcgt
      961 tcgcgcgcgc gtcgagaacc ggagggcatc ccacgcgacg gcttcggcgg cgagaggacc
     1021 tgcaatccgc gtggcgaccg ctgccgcact tgcatagaag cgatcgggat ccgcgaacgc
     1081 gcacgcacac accgcgtcgg cgggctcgta acggcggttg ccccaccctt cgatggccaa
     1141 caccgtcgcc gtctcgtcga ggatcgcttg cgtgtagcga tcgagctctc cgcgaagctg
     1201 cgcgtgcgcg tcgagcaatc cgtcgacgag ctgcgcgacg tctgcctcga gcacgaacgc
     1261 gaggaggtgc accgtcgtct gcaacagccg ctggttccag gccgcggata gcaggtggcc
     1321 aaaggcatat gcgcgccgcc agtcctcgac gggcaacgac gacgtcgcga cgatcagttc
     1381 ttctcgctcg acgacgtgca tctcgtccgc cggatctgcg ggccagctcg gcacctggcg
     1441 cgtctcgatc gcgaagcgcg cgcgatatgc cgggtcggcc atctccgcgt tttcgagcac
     1501 ccgcgtcgga tagacgaaga acgagtcgct cgccgacggc gtgagcgcag cgacgagact
     1561 gcgccggatc gatgccgcgg tctgcgccgg cagtcccagc atgagctcgt tgacggtcga
     1621 cagcccgagc gcgtgacaat gctcgcgcaa cgccagcgca tgctctggct tgatgttgtc
     1681 gcgacggatc gcagtgagga cgtcggcatc gaagtcctgc atcgagagcg ccacctgcgt
     1741 gcgaacgccg tgctcgcgca ggatctcgac ggtgcggagg ttcttctgcg tcgcgttctt
     1801 cgtcaggtga aagaacacga agcgtggcgc gtcatgcgtg ttggcgatgc ggccgatcgc
     1861 tttcgtgatc tcgacgtctc gcggccggat gccgaagttc gcgtcgacga gatagaggta
     1921 cgagatcttg cgctcgccaa tccatgcgag ctcgcgttcg atgcgctcga ggggcagctc
     1981 gttgacgcgg ctctgtgtgg cttggcccca gtcgcagaac gtgcacgcaa acggacagcc
     2041 gcgattcgtc tcgagcacga cggccgccat cgccggaatc tcatctcgcg cgcgaagctc
     2101 gtcgaacgta ccgtcgagat acggcgagcc gatcgcatcg aacgtggcga ggcgcggccg
     2161 cggcggcccg accgagatcg tcgccgcagt gcgcgtcacg acaccggcga tcgcggtgaa
     2221 gtctgccccg cgctgcgctc tctcgatgat gtcgagaaat gccagctcgc cctcgcctag
     2281 cacgagcgcg tcgagccacg gatgcgcgtc gaggaatgca gtcgcgtcgc gtcgcggtac
     2341 cgagggtcca ccggcgacga tcacgagatc cttgcgcagc agcttggcgc gacgcgcgac
     2401 ctcgagcgcg tagcgggcgt gccaggtgta gagagacaga ccgacgacgt ccgcattggc
     2461 gagtgcagct gctgcgactt cgaccggctg cctccgcacg tgaacgcgca cctcggcggc
     2521 gaccgcggcg cggcgaatcg tcgacgcgag gaggccggcg gccagcggcg gcacgtgcag
     2581 cacggtcgca ccggcgaact gcgcgatggc aacctggagc acatcccatc atagcgctgg
     2641 cgccgttcgc tcatgcgcgt tgtttacggc tgtggcggac cgccctagga tgcgccggtg
     2701 cgagctcgcc gcgaatgggt gacacgcgcg ctcgtggtgg cgggcgtcgt cgtcctcggc
     2761 gtgctgttct ccctcgcgta cggcttcgag ctcggcacgg gcaaccagca cacgtacttg
     2821 ctcgacccgc tcgtccgcgc cgacccgacg ctttatcgcc aggactggtt cgtcacgcaa
     2881 aaccaccact atcacttcgc gtttgcgacc gtgaccgccc cgctcttctc gctcgatccc
     2941 gacggagcgc gcgcattcgg catcgcacag atcctcattt cggtcgccac gtttggcgcg
     3001 atctacggcc tcgtcgcagc ggcgaccgag cgcggccgat tcacgatctt cgccggcctg
     3061 tgcgggctca tcctcctcgg aggcgaccgc gcactcggcg gcagctacct atacgcgggg
     3121 tacctgcagc cttcgtcgtt ctccgtcctc gcgtggctgc tcgcgctcaa cgcgtggctt
     3181 cgcaatcgct cggcgctcgc ggggatcgcc ctcgggatcg gcgcggtctt tcatctcaac
     3241 tacgcgctgc tcggtatcgg cgtgttcggt ctcgctgcgc tggccacgca tccgcgcgac
     3301 tggcgccgca tcgcgatgtt gctggcgccg tcgctgatcg tggtgatcgc gttcttgccg
     3361 acgatgctcg cagcttcgaa gaccagcgac gacgcgctcg cgctgcgggt cctcgtgaaa
     3421 tatttgttcc ccggccactt caagccggtg cagctgtggc tcgagctcgc gtcgctgctc
     3481 ggttggttcg tcctgctgct cgcaatcaaa accgaggatc gctcggattc gtcgtcacgc
     3541 gtcttcaact tcggcgtcgc gacgatgggc gcatgcatcc tcgccgtcgt cgtcgtctcc
     3601 attccgca
//
LOCUS       DATLBZ010000710         5138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6678841, whole genome shotgun sequence.
ACCESSION   DATLBZ010000710 DATLBZ010000000
VERSION     DATLBZ010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6678841"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /gene="pstC"
                     /locus_tag="VMZ53_34475"
     CDS             <1..771
                     /gene="pstC"
                     /locus_tag="VMZ53_34475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HUS33675.1"
                     /translation="AVVMVSRAAPVFSKMGLEFFSSKRWSAAEGIYGALPFIFGTLFT
                     AAIAVVIAVPISLGVALFITQIAPPSLRRPLVYVLDLLAVIPSVVFGLWGILVLAPHL
                     GRSFLTAGIILAIMITPIITSLAREVIETTPPGEKEAALALGATRWEMIWGSVWPHSK
                     GGLVGAVMLGLGRAMGETIAAALVIGSALGQVTLHAMKPGNSMPAVIANEWGEADALH
                     KSALIALAVVLFLITIAVNLIATSIVRRSMVRGRGKSS"
     gene            849..1604
                     /gene="pstA"
                     /locus_tag="VMZ53_34480"
                     /pseudo
     CDS             849..1604
                     /gene="pstA"
                     /locus_tag="VMZ53_34480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
     gene            1565..2404
                     /gene="pstB"
                     /locus_tag="VMZ53_34485"
     CDS             1565..2404
                     /gene="pstB"
                     /locus_tag="VMZ53_34485"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HUS33676.1"
                     /translation="MGSRAYARDVDVLSDSDRTPTVFALEALSVFYGAFRAVRDVTLG
                     IDKNQITAFIGPSGCGKTTVLRCLNRMNDLVAGARVEGRVTYHGTDLYGRDVSATEVR
                     RRIGMVFQKPNPFPKSIYDNIAFGPRINGISDRRKLDEVVEKSLRAAALWDEVKDRLR
                     HSALGMSGGQQQRLCIARTIAVEPEVVLMDEPCSALDPIATARVEELMRQLKETYTIV
                     IVTHNMQQAARVSDRTAFFTAEVDSKNDQRTGVLVEYDATAKIFSNPREERTENYITG
                     RFG"
     gene            complement(2405..3406)
                     /locus_tag="VMZ53_34490"
     CDS             complement(2405..3406)
                     /locus_tag="VMZ53_34490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33677.1"
                     /translation="MTTRLFCVLIGMLAGACTEGVSDPNLYSGPDIPADASVDDTEDR
                     HRRDAAMIDIDASVPPSSATDADRFGVKLLYPTLAGGKNWVSSWDNGQARTFTGVDPK
                     DTWFDANHGNASYKVDGNGTLKISGSVPRMYIHDPALVSQWRNVEMTMYFMRVSDSNI
                     AYGGLVGVARSNHGTAGGSETANPCDTRGAIARMRYDGHIDFEKETAHPASSTTLNKT
                     QWSGGMPKNVWIGYKHVVYDLPDGNVKQELYIDTTDGANGGTWVKLNEVVDNGTTFAV
                     GGTACKTGVDPAMKLTASPTRGGSESGKPNITVYFRSDGVGTDGLVYKKGSVREISP"
     gene            complement(3514..4197)
                     /gene="phoU"
                     /locus_tag="VMZ53_34495"
     CDS             complement(3514..4197)
                     /gene="phoU"
                     /locus_tag="VMZ53_34495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HUS33678.1"
                     /translation="MTSHTSKDFATELRTLRERLCAMGGRCEQQITLAMQALIERDDE
                     LARKVLEADEAIDRDESEIDELALQILATRQPVASDLRFITMSLKFVTDLERIGDNAA
                     GIAKRALELNHVPALDKVPDLGQLSTLVQKNLRAALDSFVTKDADRASAVIQADREID
                     KRNASLFAELLAHVATDPATVTRVLPLTSVCRYLERIGDHVKNLAEEIVYMVKAQDVR
                     HRPLPKPAN"
     gene            complement(4197..>5138)
                     /locus_tag="VMZ53_34500"
     CDS             complement(4197..>5138)
                     /locus_tag="VMZ53_34500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33679.1"
                     /translation="KIEMLNDAARRMLGVQSSLVGESLLEFIRVPELHALVERTEDAS
                     AEVQLPNGPRTLVRAARTWGRAGRVLLLEDVTTVRRLESVRRDFVANVSHELRTPVAV
                     IRANAETLIAGAKDDPQMAPKLIDGLHRNAERLARILADLLDLSRLDAGQYRLDIAPV
                     GVRAVTDQSLSAVETQAKARNVTLDIAVPPALLVRADAKALDQILVNLIDNGVKYTRP
                     EGHVWVEARDVGDAVRIEVRDDGPGIADKHRERVFERFYRADASRSREAGGTGLGLSI
                     VKHLVESMDGTVGVEPNVPRGSVFWLRLPKASTKEAA"
BASE COUNT          806 a         1693 c         1643 g          996 t
ORIGIN      
        1 gcggtcgtca tggtgagccg ggccgcgccc gtgttctcga agatggggct cgagttcttc
       61 tcgtcgaagc gttggtcagc ggccgaaggc atctacggcg cgctgccgtt catcttcggc
      121 acgctgttca ccgcggcgat cgcggtcgtc atcgccgtgc cgatcagcct cggcgtcgcg
      181 ctgttcatca cgcagatcgc gccgccctcg ctgcgccgcc cactcgtgta cgtgctcgat
      241 ctgctcgccg tcattccatc ggtcgtgttc ggcctgtggg gcatcctcgt gctcgcgccg
      301 cacctcggtc gctcgtttct caccgcgggc atcattctcg cgatcatgat cacgccgatc
      361 atcacgtcgc tcgcgcgcga ggtcatcgag acgaccccgc ccggcgagaa agaagccgca
      421 ctcgcgctcg gcgcaacgcg ctgggaaatg atctggggct cggtgtggcc acacagcaaa
      481 ggtggcctcg tcggtgcggt gatgctgggc ctcggccgcg cgatgggcga gacgatcgcg
      541 gcggcgctcg tgatcggctc cgcgctgggc caagtcacgc tccacgcgat gaagcccggc
      601 aactcgatgc ccgccgtcat cgcgaacgaa tggggcgagg ccgacgcgct tcacaagtct
      661 gcgctcatcg cgctcgcggt cgtcctgttc ctcatcacga tcgccgtcaa cctcatcgcc
      721 acgtcgatcg ttcgccgctc gatggtgcgc ggccgaggga agtcatcatg atggagctcc
      781 ggtcggccct cgaccttcgt gcccgccaca actggcgctc gacgaagaat gtgctcggcg
      841 tcgtgctcat gggcctctgc gtcgtcgtcg tcctcatccc gctcgccgcg gtgatctatg
      901 cggtggtcca caaaggcgcc ggcgtcgcgt tcaaggcgtt tcccgagttc ttcacgaagg
      961 acatcccgat catctcgcgc aaagccggtc cgggcatggg gcccgcgatc atcggcacgc
     1021 tgctcgtcac gggcggcgcg acgcttatcg ccgttccgct cggcatcctc ggcgcggtgt
     1081 acctgcacga gtacggcggc accacgaagt ttgcgcgcgt cgtgcgcttc atggcgatgg
     1141 tgatgaccgg cgtcccatcc atcgtgatgg gcctgttcgt ctacatcctg tggaccgttc
     1201 gcttcggcta ctcggcattt ggcggctcgc tcgcccttgc gtgcttgatg ttgccggtcg
     1261 tcatcggctc gacggagcag atgctgcgcc tcgtcccgaa tcagctgcga gaaggcgcgt
     1321 acgcgctggg cgcatcgaag agccgcgtga tcctcacggt cgtcattccc gcagcgctgc
     1381 cgggcatcgt ctcgggctgc ctgctcgcga tcgcacgcgc tgccggcgag accgcgccgc
     1441 tcatgttcgc agtgggcgct gcaacacgca tgaatccgca tttcttcgac gaggcaaaca
     1501 ccgccctgtc gattcagatc ttcagtaacg cgacgtccag tttcgtcgca gctcaggaga
     1561 gagcatgggg agccgcgctt acgctcgtga tgttgacgtt ctttctgact ctgaccgcac
     1621 gcctactgtt tttgccctcg aggcgctctc ggttttctac ggtgcgttcc gcgctgtccg
     1681 agatgtcaca ctcggtatcg acaagaatca gatcacggcg ttcatcggtc cctcgggctg
     1741 cggtaagacg accgtcctcc gctgcctgaa ccgcatgaac gatctcgtgg cgggtgcgcg
     1801 cgtcgagggc cgcgtcacgt atcacggcac cgatctctac ggccgggacg tgtctgccac
     1861 cgaagtgcgc cgccgcatcg gcatggtctt ccagaagccg aatccgtttc ccaaatcgat
     1921 ctacgacaac atcgcgttcg gcccgcgcat caacggcatc agcgaccgcc gcaagctcga
     1981 cgaggtcgtc gagaagtcgc tgcgcgccgc cgcgctctgg gacgaggtga aagaccgcct
     2041 acgccattcc gcgctcggca tgtccggcgg ccagcagcaa cgcctctgca tcgcgcgcac
     2101 gatcgccgtc gagcccgagg tcgtgctcat ggacgagcca tgcagcgcgc tcgatccgat
     2161 cgcgacggca cgcgtcgaag agctcatgcg ccagctcaag gagacgtaca cgatcgtcat
     2221 tgtcacgcac aacatgcagc aggctgcgcg cgtgagtgat cgcaccgcgt tcttcacggc
     2281 ggaggtcgat agcaaaaacg accagcgcac cggcgtgctc gtcgagtacg acgccaccgc
     2341 caagatcttc tcgaacccgc gcgaagaacg aaccgagaac tacatcaccg gccgattcgg
     2401 ctagctacgg gctgatctcg cgaacgctgc cctttttgta gaccaggccg tcagtgccga
     2461 cgccgtcgct gcggaagtag acggtgatat tgggcttgcc gctctccgag ccaccgcgcg
     2521 ttggcgacgc ggtcagcttc atcgctggat cgacgcccgt tttgcatgcg gtgccgccga
     2581 ccgcgaacgt cgtgccgttg tcgacaactt cgttgagctt cacccacgtg ccgccgttcg
     2641 cgccgtcggt ggtgtcgatg tagagctcct gcttcacgtt gccgtcgggc aggtcgtaga
     2701 cgacgtgctt gtagccgatc cacacgttct tcggcatgcc gccgctccac tgcgtcttgt
     2761 tgagcgtggt gctcgacgcc ggatgcgccg tctctttctc gaagtcgatg tggccgtcgt
     2821 agcgcatgcg agcaatcgcg ccgcgcgtat cgcacggatt tgcggtctcg ctgccgccgg
     2881 ccgtgccgtg gttgctgcgc gcgacgccga cgagcccgcc gtacgcgatg ttcgagtcag
     2941 agacgcgcat gaagtacatc gtcatctcga cgttgcgcca ctgactgacg agcgccggat
     3001 cgtggatgta catgcgcggc accgagccgc tgatcttcag cgtgccgttg ccatcgacct
     3061 tgtacgacgc gttgccgtga ttcgcgtcga accacgtgtc cttcggatcg acacccgtga
     3121 acgtacgcgc ttgaccgttg tcccacgacg agacccagtt cttgccgcct gcgagcgtcg
     3181 gatacagcag cttcacgccg aagcgatctg cgtccgtcgc cgatgacggt gggacactcg
     3241 cgtcgatgtc gatcatcgcg gcgtcgcgcc tgtgacggtc ctcggtatcg tcgacggatg
     3301 cgtccgccgg gatgtcaggc ccgctgtaca aattcggatc tgaaacgccc tcggtgcatg
     3361 cacccgcgag catcccgatc aagacacaga agagtcttgt cgtcatttcc cccgcgagca
     3421 cagtagctcg gtgcgcgctc acgcctcaat tggacgattt cgtgcgctcc ggcgagtgcg
     3481 agcacgcgta cgaaatttcg tcgacgaggc aagtcagttc gcgggcttgg gcaaaggccg
     3541 atgacgcacg tcctgtgcct tgaccatgta cacgatttct tctgcgagat ttttcacgtg
     3601 gtcgccgatg cgttcgaggt agcggcacac gctcgttagt ggcaacacgc gcgtgaccgt
     3661 cgctgggtcg gtggctacat gcgcgagcag ctccgcgaac aaggacgcgt tgcgcttgtc
     3721 gatctcgcgg tcggcttgaa tcaccgcaga cgcgcggtcg gcatccttcg taacgaagct
     3781 gtcgagtgca gcgcgcagat tcttctgcac gagcgtcgac agttggccga ggtcgggaac
     3841 cttgtcgagc gcgggcacgt ggttgagctc gagtgcgcgc ttcgcgatcc cagcggcgtt
     3901 gtcgccgatg cgttcgaggt cggtgacgaa cttgagagac atcgtgatga agcgcaggtc
     3961 cgaggcgacg ggctgccgcg tcgcgagaat ctggagtgct agctcgtcga tctccgactc
     4021 gtcgcgatcg atcgcttcgt cggcctcgag gaccttgcgc gccagctcgt catcgcgctc
     4081 gatcagcgct tgcatcgcga gcgtgatctg ttgctcgcac cggccgccca tggcgcagag
     4141 tcgctcgcgc agcgtgcgca gctcggttgc gaagtccttg ctcgtatgtg aggtcatcac
     4201 gctgcctcct tggtagatgc tttcggaagg cgcagccaga acacgctgcc gcgcggaacg
     4261 ttcggctcga caccgacggt gccgtccatg ctctcgacga ggtgtttcac gatcgagagg
     4321 ccgaggccgg tgccgccggc ttcgcgcgag cggctcgcat cggcacgata gaagcgctcg
     4381 aacacgcgct cgcgatgctt gtccgcgatc cctggaccgt cgtcgcgaac ttcgatgcgg
     4441 accgcgtctc ccacgtcgcg cgcttcgacc caaacatggc cttcgggccg cgtgtatttg
     4501 acgccgttgt cgatcaagtt gacgaggatc tgatcgagcg ccttcgcgtc tgcgcggacg
     4561 agcaacgcgg gcggcaccgc gatgtccagc gtgacgttgc gcgccttggc ctgcgtctcg
     4621 accgcggaca gcgattggtc ggtcaccgcg cgcacaccga cgggtgcgat gtcgaggcgg
     4681 tactggccgg cgtcgaggcg cgatagatcg aggaggtcgg cgaggatgcg cgcgagcctc
     4741 tcggcgttgc gatggaggcc atcgatgagc ttcggcgcca tctgcggatc gtccttcgcg
     4801 ccggcgatga gcgtctcggc gttcgcgcgg ataacggcga cgggtgtgcg cagctcgtgc
     4861 gaaacgttcg cgacaaaatc gcggcgcacg gattcaagcc ggcgcaccgt cgtgacgtcc
     4921 tcgaggagca gcacgcgacc ggcgcggccc cacgtacgcg cggcacggac gagcgtgcgc
     4981 gggccgttcg gcagctggac ctcggcactt gcatcttccg ttcgctcgac gagcgcatgc
     5041 agctctggaa cgcggatgaa ctcgagcagc gattctccga cgagcgagct ctggacgccg
     5101 agcatccggc gcgctgcatc gttgagcatc tcgatctt
//
LOCUS       DATLBZ010000711         4152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6688737, whole genome shotgun sequence.
ACCESSION   DATLBZ010000711 DATLBZ010000000
VERSION     DATLBZ010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6688737"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VMZ53_34505"
     CDS             <1..703
                     /locus_tag="VMZ53_34505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33680.1"
                     /translation="HSVLEEFQALGYPILSMRSLPRDKATMDRLFAEDIAKGVIEDGL
                     DVTDVWPENYSTNSVQKVWAAKYASRHPNLACLDLSSFKCGHDAPTYGLIDSIIASAG
                     KAYSALHDIDANKPSGSIKIRVKTYAYTLMLLKERLEDQAVKKSELDRHMAMKKLELM
                     VNLQQKLASVGRTDDKLDAEIGALAAQAAEWRAQDEAKNPKAKKLASLNVIKDGSGAG
                     SSSSANLAKANKETK"
     gene            700..2568
                     /locus_tag="VMZ53_34510"
     CDS             700..2568
                     /locus_tag="VMZ53_34510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HUS33681.1"
                     /translation="MSTTNDKPGSSLDTLSLENIEEQLKQFEIEERKRLGLPVENAKH
                     WFDAVPREFTRDQREHTTILVSGLTMAHDLFIQAALRGIGYKVLAMDTPDNDALQYGR
                     EYGNRGQCNPTYFTVGNLVKYLDGLRERGMSKDEIVKNHVFITAGACGPCRFGTYVTE
                     YRKALRDSGFDGFRVLLFQQTGGLKQATGDGVGLEMNPTFFWAVVRAILIGDSLNALG
                     YRIRPYEVEAGAADRALEQSKKIISDAFENNTSLPLALYKVRRVLSKVKVDRTKPKPR
                     VGIIGEFWAMTTEGDGNYGLQRFLEAEGAEPDIQIVTAWLLYMIWQGRYDTKQRATLR
                     GADKAFKDADGKQAVGGSKFSLAGVDVRKRMASLWAGEKVLHGLFQTIAKAMGLADYH
                     LPNMEEIAEVSHAFYDNNLRGGEGHMEIGKLIQNVAYSKVNMTLSVKPFGCMPSSSVS
                     DGIQSFITELYPQGIFLPIETNGDGAVNVYSRVQMQLFKAKQLAQKEVDKALADVGMT
                     MDEVRAYLRKHPSMMSPFHKSPHMAGCTTADLVYEVGARKNRLSFLKAKVVGLLTGKQ
                     AKEHEEAHVKLLQVARNAAAKTRKKNSVVSVAPEVDPEDQAAPPIPAGRKSLRVVN"
     gene            2668..>4152
                     /locus_tag="VMZ53_34515"
     CDS             2668..>4152
                     /locus_tag="VMZ53_34515"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33682.1"
                     /translation="MRVGRCLWVAVALAALCVGCDDQDGVRHLDAWVDTPIADADVDA
                     TPVDAANVTVAASASTGGTVTVTVVTDGTCSGTSCTLATGASTVTFTPTASTGYRFDT
                     WTGSSCTGYTASGDAITFTNPIVDHACTASFVATVDVVAVAGAGGSIVATSVTGGVCT
                     GTACTLDAGASTVTFTPTAAANHAFSGWSGCTGYTQSGDSITFTNPTSAKACTASFGA
                     TVAISATAGTGGTVAASSVTGGGCSGTSCTLDSGASTVTFTPTPNANYRFNSWSGCTG
                     YTQSGNAITFTNPTTPQSCTASFVATVSVSATAGTGGSVAAGTPTGGTCVGTTCALDS
                     GASSVTFTPTANANYRFGGWTGCTGYTQSGNAITFTNPTAAKACTANFVATVIVSASA
                     GTGGTVAASTPTGGTCAGTTCTLDSGASSVTFTPTANANFRFGGWSGASCTGYTTAGD
                     SITFTNPATAKACTASFVATVNVSASAGTGGTVAAGAPAGGTCTG"
BASE COUNT          785 a         1345 c         1375 g          647 t
ORIGIN      
        1 tcactcggtg ctcgaggagt tccaggcgct cggctatccg atcctgtcga tgcggtcgtt
       61 gccgcgcgac aaggccacga tggacaggct gttcgccgag gacatcgcga agggcgtgat
      121 cgaagacggc ctcgacgtca ctgacgtgtg gccggagaac tactcgacga actcggtcca
      181 gaaagtctgg gccgcgaagt acgcgtcgcg tcacccgaac cttgcgtgtc tcgatctctc
      241 gagcttcaag tgcggtcacg acgcgccgac gtacggcctg atcgattcga tcatcgcgag
      301 cgccggtaag gcgtactcgg cgttgcacga catcgatgcg aacaagccga gcggctcgat
      361 caagattcgc gtcaagacgt acgcgtacac gctgatgctc ctcaaggaga ggctcgagga
      421 tcaggccgtc aagaagagcg agctcgatcg ccacatggcg atgaagaagc tcgagctgat
      481 ggtcaacctc cagcagaagc tcgcaagcgt cgggcgcacc gacgacaagc tcgacgcgga
      541 gatcggggcg cttgctgccc aggccgccga gtggcgcgct caggacgaag ccaagaaccc
      601 gaaggcgaag aaactcgcct ctctcaatgt gatcaaggac ggaagcggcg ctggaagcag
      661 ctcctctgcg aacctagcga aggccaataa ggaaaccaaa tgagcacgac gaacgacaag
      721 cccggtagca gcctcgacac cctctcgctc gagaacatcg aggagcagct caagcagttc
      781 gagatcgagg agcgcaagcg tcttggcctc ccggtcgaga atgcgaaaca ttggttcgat
      841 gcggttccgc gtgagttcac ccgcgatcag cgcgagcaca cgacgattct cgtctccggc
      901 ctcacgatgg cgcacgacct gttcatccag gccgcgcttc gcggcattgg ctacaaggtc
      961 ctcgcgatgg acacgccgga taacgacgcg ctccagtacg gacgcgagta cggcaaccgc
     1021 ggccagtgca acccgacgta cttcaccgtc ggcaatctcg tgaagtacct cgacggcctg
     1081 cgcgagcgcg gcatgtcgaa ggacgagatc gttaagaacc acgtgttcat cacggccggc
     1141 gcgtgtggcc cgtgccgctt cggcacgtac gtcaccgagt accgcaaggc gctccgcgac
     1201 tccggcttcg acggcttccg cgtcctgctg ttccagcaga cgggcggtct caagcaagcg
     1261 accggcgacg gcgttggcct cgagatgaac ccgacgttct tctgggccgt cgtccgcgcg
     1321 atcctgatcg gtgactcgct caacgcgctc ggctaccgca ttcgcccgta cgaggtcgag
     1381 gccggcgctg ccgaccgcgc gctcgagcag agcaagaaga tcatcagcga cgcgttcgag
     1441 aacaacacgt cacttccgct cgcgctctac aaggtgcgtc gcgtgctcag caaggtgaag
     1501 gtcgaccgga ccaagccgaa gccgcgcgtt ggcatcatcg gcgagttctg ggcgatgacg
     1561 accgaaggcg atggcaacta cggcctgcag cgcttcctcg aggccgaggg cgccgagccg
     1621 gatatccaga tcgtcaccgc gtggctgctc tatatgatct ggcagggccg ctacgatacg
     1681 aagcagcgcg ccactctccg cggcgccgac aaggcgttca aggatgccga tggcaagcag
     1741 gccgtcggag gctcgaagtt cagcctcgcg ggcgtcgacg tccgcaagcg catggcgtcg
     1801 ctgtgggccg gcgagaaggt tctgcacggc ctcttccaga cgatcgcgaa ggcgatgggc
     1861 ctggcggatt accacctgcc gaacatggag gagatcgccg aggtctcgca cgcgttctac
     1921 gacaacaacc tccgcggtgg cgaaggccac atggagattg gaaagctgat ccagaacgtc
     1981 gcgtactcga aggtcaacat gaccctctcc gtgaagccgt tcggatgcat gccttcgtcg
     2041 tcggtctccg acggtatcca gtcgttcatc accgagctct atccgcaggg catcttcctg
     2101 ccgatcgaga ccaacggtga cggcgccgtc aacgtgtaca gccgcgtgca gatgcagctg
     2161 ttcaaggcca agcagctcgc gcagaaggaa gtcgacaagg cgctcgccga cgtcggcatg
     2221 acgatggacg aagtgcgtgc gtacctccgc aagcacccgt cgatgatgag cccgttccac
     2281 aagtcgccgc acatggcggg ttgcacgacg gccgacctcg tctacgaagt gggcgcgcgc
     2341 aagaatcggc tctcgttcct caaggcgaag gtcgttggtc tgctcactgg caagcaagcc
     2401 aaggagcacg aggaggcgca cgtcaagctt ctccaggtcg cgcgcaacgc agccgccaag
     2461 acgcgcaaga agaacagcgt cgtctcggtt gcgcctgagg tcgatccgga agatcaggca
     2521 gcgccgccga tcccggccgg ccgtaagagc ctccgcgtcg taaattaggc aatcccgctc
     2581 acgtcgcaga cagcccgccc gtcgggctgt ttgtgttttc ggcgtacgag cgtgcagtcc
     2641 tagagcgcgt gtgtagtatc cgccgccatg cgcgttggcc gctgtttgtg ggtcgctgtt
     2701 gcgttagctg cgttgtgcgt cggttgtgac gatcaagatg gcgtccgcca tctcgatgcg
     2761 tgggtcgaca cgccgatcgc cgatgcggat gtcgacgcga cgcccgtcga tgccgcgaac
     2821 gtcacggtcg cggcgagcgc gagcacgggc ggcaccgtca ctgtcacggt ggtgaccgat
     2881 ggcacgtgca gcggcacgtc atgcacgctc gcgaccggcg cgtcgacggt gacgttcacg
     2941 ccaaccgcga gcacgggcta tcgcttcgac acgtggaccg gctcgtcatg cacgggctac
     3001 acggcatcgg gcgatgcgat cacgttcacg aatccgatcg tcgatcacgc gtgcaccgcg
     3061 tcgttcgtcg cgacggtcga cgtcgtcgcg gtcgcaggcg cgggcggcag catcgtcgcg
     3121 acctcggtca ccggcggcgt gtgcacgggc acggcgtgca cgctcgacgc gggcgccagc
     3181 acggttacgt tcacgccgac cgccgccgcg aatcacgcgt tcagtggttg gtcgggttgc
     3241 acgggctaca cgcagagcgg cgactcgatc acgttcacga atccgacgag cgcgaaggcg
     3301 tgcaccgcga gcttcggcgc gactgtcgcg atctccgcga cggcgggaac cggcggaacc
     3361 gtcgctgcgt cgagcgtgac gggtggtggc tgctccggca cgagctgcac gctcgacagc
     3421 ggcgcgagca ccgtgacgtt cacgccgacg ccgaatgcga actaccgctt caacagctgg
     3481 tcgggctgca cgggctacac gcagagcggc aacgcgatca cgttcacgaa cccgacgacg
     3541 ccgcagtcct gtacggcaag ctttgtcgcc accgtcagtg tctcggcgac ggcagggacg
     3601 ggcggcagcg tcgcggcagg cacgcccacc ggcggcacct gcgtcggtac gacgtgcgcg
     3661 ctcgatagcg gcgcgagcag cgtgacgttc acgccgaccg cgaacgcgaa ctatcgcttc
     3721 ggtggctgga ccggctgcac gggctacacg cagagcggca acgcgatcac gttcacgaac
     3781 ccgacggccg cgaaggcatg cacggccaac ttcgtcgcga cggtgatcgt ctcggcgagt
     3841 gccggcacgg gcggcacggt cgcggcgtcg acgccgacgg gcggcacgtg tgcgggcacg
     3901 acgtgcacac tcgatagcgg cgcgagcagc gtgacgttca cgccgacggc aaacgcgaac
     3961 ttccgcttcg gcggctggtc gggcgcgtcc tgcacgggct acacgacggc cggcgactcg
     4021 atcacgttta cgaacccagc gaccgcgaag gcatgcaccg cgagctttgt cgcgacggtc
     4081 aacgtctcgg cgtcggcagg taccggcggc acggtggcag caggagctcc ggcgggcggc
     4141 acgtgcaccg gc
//
LOCUS       DATLBZ010000712         6721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6695035, whole genome shotgun sequence.
ACCESSION   DATLBZ010000712 DATLBZ010000000
VERSION     DATLBZ010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6695035"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VMZ53_34520"
     CDS             complement(<1..643)
                     /locus_tag="VMZ53_34520"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HUS33683.1"
                     /translation="MYLQDLIFTLSKFWADRGCVIEQPVDVEVGAGTFHPATFLRVLG
                     PEPWNAAFVQFCRRPSDGRYGENPNRAGAYFQFQVGLKPSPLHVQDLYLDSLRAIGMD
                     PASHDIRFVEDDWESPTLGAWGLGWEVWCDGMEVTQFTYFQQAGGIDLLPVTAELTYG
                     IERLAMYLQGVSSMYDLKWDKHVTYGQIRHPWEVQYSTYHFEQLDPQIAFRNFD"
     gene            752..1255
                     /locus_tag="VMZ53_34525"
     CDS             752..1255
                     /locus_tag="VMZ53_34525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33684.1"
                     /translation="MVREYVPLAFAALLPGCSLILDFSDSAAPHDAAIDAVYTQEQCD
                     YLEPNDSLQSPVMVTQADTGTAAICAPVMGGSDDHDFYRFSAGAVPFSVGIQFTNRPG
                     GDLDMKVYNAADGTLLGQSRGFGDGETLTCPGTSPACPSVSPGDFVVEVFPALAGSVN
                     TYKLVLP"
     gene            complement(1252..2520)
                     /locus_tag="VMZ53_34530"
     CDS             complement(1252..2520)
                     /locus_tag="VMZ53_34530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33685.1"
                     /translation="MQRALAAFATLALGACHPHGSPKNAKAPEAGVSIAVYEADGAGY
                     SVIDDRRFIELGNQTSVLISNIDPGADLASLVLEPTSSALHIGSCTRDRLPEPPFVDT
                     LEEYSRKQAELQLALERERRIRAIRGEPMPAPPAPPRTGSTDTPARYAPVVRCEVTGA
                     PGKYLVRMLYVSTTLRYRAQHDVELSADGTKATVITRFALQTPNWRSRADVVLFDGVP
                     GGEHAPKEVTRGGVELDGTVAILAVDPKQTTAEMRRVYDGAVMMGDDEKDPMWGRESQ
                     NSIWVWLELAALKLAPGPVHIHMLEADGPRDIDVPATSRKQEDEPNAPLRLPLWADDD
                     LRGNRTRTVEYNDGNEVTERLVFSIANMGETTREVFIEERLRTATKRTIENPWPKKAT
                     SAAPGDLLRTKLDAKPNKVTRAGYTLTYVF"
     gene            complement(2521..3501)
                     /locus_tag="VMZ53_34535"
     CDS             complement(2521..3501)
                     /locus_tag="VMZ53_34535"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HUS33686.1"
                     /translation="MKRADTVGIVGAGAFGTALGSVIARAGRRVILWTRDQEVATAIA
                     NQRKCPRLPEAELPEPLEVTTDPNRLASEARFLVMAVASTDVRDRAKELGNYVDGSHI
                     IVHAIGALATPNNERVSEVMAQGLPTLKIGVIAGPALPGDLAVGEFSSMVVASVFDEV
                     VAEARRLLNAPPALRVYGSKDLAGVELASALSGAYTVALGLCDGLGMGVGPRAVLVTR
                     IVAEASRLAQAAGAEARTFAGLSGLGNMLVRSTEKSADYQLGKRVADGVLSADTQRTE
                     GARAALAGAALAKSLKVRMPVLTGVAAVLAGKAEPREAASLVGDNVAVEE"
     gene            complement(3501..4964)
                     /locus_tag="VMZ53_34540"
     CDS             complement(3501..4964)
                     /locus_tag="VMZ53_34540"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HUS33687.1"
                     /translation="MLNDVAYCEIKRLEKSKNGAADRWRDLSRRLLTMTEEDKQQELR
                     GLVRYYGRDVVGNFDPRVYKFATGVGPSILGMVFSPMSTIKEGMASLKNLDQRIHADG
                     ETDIARACAERGTIVVTPTHSSNMDSPAIGFGLMRAGLPPTTYGAGKNLFTNPFISFF
                     MRNLGAYRVDRRLRFELYKDVLKEYSTVLLEHGYHSLFFPGGTRSRSNLVEKHLKLGL
                     LGTTATAYKNLVREGMPNKRLYIVPATINYRLVLEAETLVDDYLAETGKSRYIITDDE
                     FSRLGRIVEFFRKILAHEGSVIVRFGRPLDPFGNDITDDGESIDRAGRIVDPASFVRG
                     ADGEVIDDDQRDAEYTRALGRRLAQVYPKLTVFHSTHLLARSLYDAVSRAAGTRDVYR
                     LMRAAPPQLAVTVADATAGVDRLRARLLEHPEWGAEHPNYTTQSSDQKVDDALRGLST
                     YHTRPIVERTGDALHVRDIKLLFYYFNRTAHIPAETA"
     gene            5256..>6721
                     /locus_tag="VMZ53_34545"
     CDS             5256..>6721
                     /locus_tag="VMZ53_34545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HUS33688.1"
                     /translation="MTKRPATRSKKTAEPAKAKAKAPRRSKANEDEDPAPKKAKAAKA
                     EKAEKPAKAAEKPTKAAEKPAEKLADKPAEQSADKPVDKPVKASRSKKAAVEAAPPAP
                     PKPVYTLDGPVPAEGASLPKKVGALLGVGQQTLGIKSFRPGQAEAFDHLLANEDLLAV
                     MPTGSGKSLLYQLTSLVVPGVTVVVSPLIALIKDQLDKMVAKGVTAVKIDSTLTVKQR
                     REVDALVRSPGGKLLLTTPERMADPDFRVFLRESAGGVGVSRFVVDEAHCVSQWGHDF
                     RPAYLSLRKALEDVGRPPVLATTATAPPHVRDDILFQLGMEKATIVTTTFDRPNLHYE
                     VIIFHDEDEKMRTLVTLLKKLPRPGIVYCATVKKVEELYEALTRWGIPTGRYHGRMSK
                     NERDASQNAFMASTDLIMVATNAFGLGVDKANIRNVLHYHVPGSLEAYAQEAGRGGRD
                     GKPARCVLLFSPDDVAIQEYFLSGTYPTKRQVKMVFDAL"
BASE COUNT         1161 a         2188 c         2226 g         1146 t
ORIGIN      
        1 tgtcgaagtt gcggaacgcg atctgcgggt cgagctgctc gaagtgatac gtcgagtact
       61 gcacttccca cggatggcga atctggccgt aggtgacgtg cttgtcccac ttgaggtcgt
      121 acatcgacga cacgccctgc aggtacatcg cgaggcgctc gatgccgtac gtcagctcgg
      181 cggtgacggg cagcagatcg atgccgcctg cttgctggaa gtacgtgaac tgcgtgacct
      241 ccatgccgtc gcaccagact tcccagccga ggccccacgc gccgagtgtc ggcgactccc
      301 agtcgtcctc gacgaagcgg atgtcgtggc tcgcgggatc catgccgatc gcgcgcagcg
      361 aatcgagata gagatcctgc acgtgcagcg gcgacggctt gaggccgact tggaactgga
      421 agtacgcgcc ggcgcggttc gggttctcgc cgtagcggcc atccgacgga cgacggcaga
      481 actgcacgaa cgcggcattc cacggctcgg gaccgagcac gcgaaggaac gtcgccggat
      541 gaaacgtgcc ggctccaacc tcgacgtcca cgggctgctc gatcacgcat ccgcgatcgg
      601 cccagaactt actgagcgtg aagatgaggt cttgcaggta catgggccgg cggcacccta
      661 ccgtcggcag cgtcgacggt caagcacggg tcccgagcta agtgcgccag aatacttgac
      721 gatcttccca tcgcggacgt agcgtgacga catggtccgg gagtatgtgc cgctagcgtt
      781 cgccgcgctc ctacccggct gctcgctcat tctcgatttc tccgactccg ctgcgccgca
      841 cgacgccgcg atcgacgccg tgtacacgca ggagcaatgc gattacctcg agccgaatga
      901 ctcgctgcaa agcccggtga tggtcacgca agccgacacc ggcacggcgg cgatctgcgc
      961 accggtgatg ggcggctccg acgaccacga cttctatcgc tttagcgccg gcgcggtgcc
     1021 gttctccgtc ggcatccagt tcacgaatcg tccgggcggt gacctcgaca tgaaggtcta
     1081 caacgcggcc gacggcacgc tgctcggtca gtcgcgcggc ttcggcgacg gcgagacgct
     1141 cacgtgcccc ggcacatcgc cggcgtgtcc gagtgtgtcg cccggcgatt tcgtcgtcga
     1201 ggtgttccca gcgctcgccg gctcggtgaa cacgtacaag ctcgttctgc cttagaacac
     1261 gtacgtcagc gtgtagcccg cgcgcgtgac cttgttcggc ttcgcgtcga gcttcgtccg
     1321 cagcagatcg ccaggagcgg cggacgtggc tttcttcggc cacgggtttt cgatcgtgcg
     1381 cttcgtcgcg gtccgcaggc gctcttcgat gaacacctcg cgtgtcgtct cgcccatgtt
     1441 cgcgatgctg aacacgaggc gctcggtgac ctcgttgcca tcgttgtact cgacggtgcg
     1501 cgtgcggttg ccgcgcagat catcgtcggc ccacagcggc aggcgaagcg gcgcgttcgg
     1561 ctcgtcctct tgcttgcggc tcgtggccgg tacatcgata tcgcgcgggc cgtcggcctc
     1621 gagcatgtga atgtgcacgg gccccggcgc gagcttcagc gcggcgagct cgagccacac
     1681 ccagatcgag ttttgcgact cgcggcccca catcggatct ttctcgtcgt cgcccatcat
     1741 cactgcaccg tcgtagacgc gacgcatttc ggcggtcgtt tgcttcggat cgaccgcgag
     1801 gatcgcgacc gtaccgtcga gctcgacgcc accgcgcgtg acttctttgg gcgcatgctc
     1861 gccacctggg acgccgtcga acagcacgac gtcggcgcgc gaccgccagt tcggggtctg
     1921 gagcgcgaag cgagtgatga ccgtcgcctt cgtgccgtcg gcggagagct cgacatcgtg
     1981 ttgcgcgcga tagcgcagcg tcgtcgacac gtagagcatg cgcacgaggt acttgcccgg
     2041 cgcgccggtg acctcgcagc gaacaacagg tgcgtagcgc gcaggcgtgt ccgtcgaacc
     2101 cgtgcgcggc ggcgcgggag gtgctggcat cggctcgccg cggatcgcgc ggatgcgacg
     2161 ttcgcgttcg agtgcgagct gcagctcggc ttgcttgcgg ctgtactcct cgagcgtgtc
     2221 gacgaacggc ggctcgggta agcgatcgcg cgtgcagctg ccgatgtgca gcgcagagct
     2281 cgtcggctcg agcacgagcg acgcgaggtc ggcgcctgga tcgatgttcg agatcaagac
     2341 cgacgtctgg ttgccgagct cgatgaatcg ccggtcgtcg atgacggagt agcccgcgcc
     2401 gtcggcttcg tacaccgcga tcgaaacgcc ggcttcggga gccttcgcgt tcttcggcga
     2461 gccatgcgga tggcaggcgc cgagcgcgag cgtcgcgaac gccgccagcg cgcgctgcat
     2521 ttattcctcg accgcgacgt tgtcgccgac gagcgatgca gcctcgcgcg gctctgcttt
     2581 gcctgcgagc accgcggcca cgccggtgag cacgggcatg cgaactttca gcgactttgc
     2641 gagcgcggca ccggcgagcg cagcacgagc gccttcggtg cgctgcgtat cggcgctgag
     2701 cacgccgtcg gcgacgcgct tgccgagctg gtagtcggcc gatttttccg tcgagcgaac
     2761 gagcatgttg ccgagccccg agaggcctgc aaacgtccgc gcttcggcac cggctgcctg
     2821 cgcgagccgc gatgcttctg cgacgatgcg agtgacgagc accgcgcgcg ggccgacacc
     2881 catgccgagg ccatcgcaca agccgagcgc gaccgtgtag gcacccgaca gcgcggatgc
     2941 cagctcgacg ccggcgagat ccttcgagcc gtagacgcgc aatgccggcg gcgcgttgag
     3001 caagcggcgc gcttccgcga ccacttcgtc gaacacagac gcgaccacca tcgacgagaa
     3061 ttctcccacg gcaaggtcgc cgggcaaggc cggaccagcg atcacgccga tcttcagcgt
     3121 gggcagacct tgggccatga cctcggacac ccgctcgttg ttcggcgttg caagagcgcc
     3181 gatcgcgtgc acgatgatgt gactgccgtc gacgtagttg ccgagctcct tcgcgcgatc
     3241 gcgcacgtcc gtcgaggcaa cggccatgac gagaaaccgc gcttcgctcg cgagccggtt
     3301 tggatccgtc gtgacctcga gcggctcggg cagctccgct tcgggaagcc gcgggcattt
     3361 gcgctggttc gcgatcgcgg tagcgacctc ttgatcgcgc gtccacagaa tgacgcgtcg
     3421 acctgcgcgc gcgatcaccg acccgagcgc cgtgccgaat gcgcccgcac cgacgatacc
     3481 gacagtatcc gcgcgcttca tcatgcagtc tccgcaggta tgtgcgccgt gcggttgaag
     3541 tagtagaaga gcagcttgat gtcgcgcacg tgcagcgcat cgccggtgcg ctcgacgatc
     3601 ggccgcgtgt gatacgtcga gagaccgcga agggcgtcgt cgactttctg gtcgctcgac
     3661 tgcgtcgtgt agttcggatg ctcggcgccc cactcgggat gctcgagcag acgcgcgcgc
     3721 aatcgatcga cgccggcggt cgcgtccgca acggtgaccg cgagctgcgg cggcgccgcg
     3781 cgcatcaagc gatacacgtc gcgggtgccg gctgctctgc tcactgcatc gtagagcgag
     3841 cgcgcgagca ggtgcgtcga gtggaagacg gtcagcttcg gatacacctg ggcgaggcgt
     3901 cgaccgagcg cacgcgtgta ctccgcatcg cgctgatcgt cgtcgatgac ttcgccgtcg
     3961 gcaccgcgga cgaagctcgc cggatcgacg atgcgccccg ccctgtcgat cgattcgccg
     4021 tcgtcggtga tgtcgttgcc gaatggatcg agcggacgcc cgaaccgaac gatcaccgag
     4081 ccctcgtgcg cgaggatctt gcggaagaac tcgacgatgc gaccgagccg gctgaattcg
     4141 tcgtcggtga tgatgtagcg gctcttgccg gtctcggcga ggtagtcgtc gaccagcgtc
     4201 tcggcttcga ggacgagccg gtagttgatc gtcgcgggca cgatgtagag ccgcttgttc
     4261 ggcatgccct cgcggacgag gttcttgtac gccgtcgcgg tggtgccgag caggccgagc
     4321 ttgaggtgtt tctcgacgag gttcgaccgg ctgcgcgtgc cgcccgggaa aaacagcgag
     4381 tgatagccgt gctcgaggag gaccgtcgag tactccttga ggacgtcttt gtagagctcg
     4441 aagcgcagcc gccgatcgac gcggtacgcg ccgaggttgc gcatgaagaa ggagatgaac
     4501 ggattggtga agaggttctt gccggcgccg tatgtcgtcg gtggcaagcc cgcacgcatg
     4561 aggccaaagc cgatcgccgg cgagtccatg ttcgacgagt gcgtcggcgt gacgacgatc
     4621 gtgccgcgct ccgcacatgc ccgcgcgatg tcggtctcgc cgtctgcgtg aatgcgctga
     4681 tcgagattct tcagcgacgc catgccctct ttgatcgtgg acatcggcga gaacaccatg
     4741 ccgaggatcg acggtccgac cccggtcgcg aacttgtaca cacgcggatc gaagttgccg
     4801 acgacgtcgc ggccgtaata gcgaacgagg ccgcgcagct cttgctgctt gtcctcctcc
     4861 gtcatcgtga gaagccggcg agaaagatcg cgccagcggt ccgccgcgcc gttcttgctc
     4921 ttctcgagac gcttgatctc gcagtacgca acgtcgttca gcacgtactc gagcgacgcg
     4981 tcgccgccgg cacgagattc ggcaattctc cgagcaacca cgcggcgctc gacctctgaa
     5041 aggagccgct cgcggtcggc gttgaacctc tcgagacggt tttcggcctc ccggaccggc
     5101 cgatcgctgg ccatacagat cgtgtaacac accgctttgg ccggaagatc gacgcgtgcc
     5161 cccttgcgga agggcgctat ggtatgttgt tcattaagtg gcgggcggtt ctctctagag
     5221 ctgcccgctc tgacccagga aggatcctgg tccttttgac caagcgtccg gcgactcgaa
     5281 gcaagaagac tgccgagcca gcgaaagcga aggctaaggc acctcgtcgc agtaaagcga
     5341 acgaggatga agatcctgcg cccaaaaagg cgaaagcggc gaaggctgaa aaagccgaga
     5401 agccggccaa ggcagcggag aagccgacca aggcagcgga gaagccggcc gaaaagctgg
     5461 cggacaagcc ggctgaacag tcggcagaca aaccggtcga caagccggtg aaagcgtcgc
     5521 gcagcaagaa ggccgcggtc gaagccgcac cgccagcgcc gccaaagccc gtctacacgc
     5581 tcgacggtcc ggttcccgcg gaaggcgcga gcctgccgaa gaaagtcggc gcgctgctcg
     5641 gcgtcggcca gcagacgctc ggcatcaagt cgttccgtcc cggtcaagcc gaggcattcg
     5701 atcacctcct cgcgaacgag gacctgctcg ccgtcatgcc gacgggcagc ggcaagtccc
     5761 tcctctatca gctcacgtcg ctcgtcgttc ccggcgtcac cgttgtcgtc agcccgctga
     5821 tcgcgctcat caaagatcag ctcgacaaga tggtcgccaa gggcgtcacc gcggtgaaga
     5881 tcgactcgac gttgacggtg aagcagcgcc gcgaagtcga cgcgctcgtg cgatcgcccg
     5941 gcggcaagct cctgctgacc acgcccgagc gcatggcgga tccggacttc cgcgtgttcc
     6001 tgcgcgagtc agccggcggc gtcggcgtgt cgcgattcgt cgtcgatgaa gcgcactgcg
     6061 tctcgcagtg gggccacgat ttccgtcccg cgtatctctc gctgcgcaaa gcgctcgagg
     6121 atgtcggccg cccgcccgtt ctcgcgacga ccgccacggc gccgccgcac gtccgcgacg
     6181 acattctgtt ccagctcgga atggagaagg cgacgatcgt cacgacgacg ttcgatcgtc
     6241 cgaacctcca ttacgaagtg atcatcttcc acgacgaaga cgagaagatg cgcacgctcg
     6301 tcacgctgct gaagaagctg ccgcgaccgg gcatcgtcta ctgcgcgacc gtcaagaagg
     6361 tggaggagct ctacgaagcg ctgacgcgct ggggcatccc gaccggcagg taccacggtc
     6421 gcatgagcaa gaacgaacgc gacgcatcgc agaacgcgtt catggcgtcg acggacctga
     6481 tcatggtcgc gacgaacgcg ttcggtctcg gcgtcgacaa ggcgaacatc cgcaacgtcc
     6541 tgcactatca cgtgcccgga tcgctcgagg cctacgcgca ggaagccggc cgcggtggtc
     6601 gcgatggcaa gccggcgcgc tgcgtgctgc tgttctcgcc ggacgacgtc gcgattcagg
     6661 agtacttcct cagcgggacg tatccgacga agcgtcaggt caagatggtg ttcgacgcgc
     6721 t
//
LOCUS       DATLBZ010000713        11300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6697178, whole genome shotgun sequence.
ACCESSION   DATLBZ010000713 DATLBZ010000000
VERSION     DATLBZ010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6697178"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VMZ53_34550"
     CDS             <1..699
                     /locus_tag="VMZ53_34550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUS33689.1"
                     /translation="SVGVMSAQVAHEINNPLTTVLGYAKLLLEDKPEGHPDRNALELI
                     AEEAERMKKIIGGLLEYARAPRPSEPLTHVAAGASAPGASEPSAVVRHVAALVAPQLK
                     RARASLVTEVPTGKPISIEAHALQQVLVNLVQNAAQALSDGGTITISSKEVAGGIATQ
                     ITVADDGPGIPAADRTRVFDPFFTTKAPGTGTGLGLAVCRHLVATAGGSIEAHEGPGG
                     KGAEFRVVIPNVSS"
     gene            760..1701
                     /locus_tag="VMZ53_34555"
     CDS             760..1701
                     /locus_tag="VMZ53_34555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33690.1"
                     /translation="MPVVIEVVARLGDTTLDHDHIPPGSTYHLGTTALVAKPGTHRVG
                     LVEITMERTFRVETPVSRPRLTWRPLIYVLGSFALHMTIWTIAMLATPIEQMEVAPID
                     LRPRFVVHIEDRTPPPPKPQHDIQARQPTESGAAAVAKQKMPEHGATPTAADMGDAAA
                     KAIARVARSVPDVNKAMEGTGPLYSEDDATAKGFGGHLWDINSDPDYATIKTGPGYNL
                     YEAAYSGEWDLGMTKQEKALSKIKAQTALAHRAAIRGDCVPSHRIAKWLHKLDEAAFK
                     EIYMGDPEIVWCLGQPVPPVFVKADAQEGLKSAIKPR"
     gene            1732..2577
                     /locus_tag="VMZ53_34560"
     CDS             1732..2577
                     /locus_tag="VMZ53_34560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33691.1"
                     /translation="MKSGTLAGEIVAGHELMHLLAVGGMGQVYLARHLRLGIIRAVKV
                     IHAELLEQDRSRERFEREAHVLARLQHNSIVHIVEYGSLDNGMPFLAMEYIDGPTLER
                     LVEDAPLPLPSALVVLSQLANAINYAHSCGVIHRDLKPSNVLVRGGDVRQLKIIDFGL
                     AHVINSDAPRLTDEKQMIGSVTYMAPEQVERIREVTTAVDIYGLGGIAYRLISGSPPF
                     KYSAPIRLMTARLSEEPEPLAARCPDIPAALDSLLTRCLAREPRQRPSGEELITELER
                     LSRAR"
     gene            2610..3953
                     /locus_tag="VMZ53_34565"
     CDS             2610..3953
                     /locus_tag="VMZ53_34565"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUS33692.1"
                     /translation="MSRILVVDDERGIRALCSDVLGRAGHEVETVDSAAGALAAVARK
                     KLDLILCDINLPDQDGVSILPKLLAGETPPAVLLITAYPSIDTAVRGMKLGARDYIGK
                     PFSPDELRLVVQRALAEDELRRKHGELTKRLAYGSMIGESLAMQQMRSTIDKVAHSDA
                     TVLVTGESGTGKELVARALHFAGRRAGGAFIPVNCGALVGTLLDSELFGHVRGAFTGA
                     ETSKRGLFVAADGGTLFLDEIGELPLELQPKLLRALQDGEVKPVGGTTSIRVDARVIA
                     ATNRTLQDQITAGAFREDLYYRLAVITIEVPALRNRPGDIPLLARHFAEQAALRAERG
                     RVTLTDAALAHLASQPWPGNVRELENAIERAVILAAGDILDVSDVAGPRSAVTAPTFA
                     TFSGDHVPTLDELERAHILKVLELCEGQKTKAAQMLGINRTTLWKKLRQYGLDAD"
     gene            complement(3950..4936)
                     /locus_tag="VMZ53_34570"
     CDS             complement(3950..4936)
                     /locus_tag="VMZ53_34570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUS33693.1"
                     /translation="MEDLVDTQDGDDDERLFELVITAEQAGGRLDAVIASALAALSRA
                     QVQRLIEDGHVSVGGAVASKSLRVKQGDTIEVTVPAPEPLEVLPEPIPLVVLYEDPDL
                     IVIDKPAGLVVHPAAGHARGTLVNALLHHCKDLAGIGGVLRPGIVHRLDKDTSGVMVA
                     TKTDRAHAALTAAFAAKSRGEPGGLERTYLAITSPAPPTKTGTLRTQYGRHPVHRKKF
                     SSKVSGGKSAVTHWTVEEPLHEAALVRFRLETGRTHQIRVHASDHGWALIGDQVYGHK
                     PRDERTADVATKLGRQALHAATLAFAHPATGDELEFESALPEDMQSALAALR"
     gene            complement(4921..5187)
                     /locus_tag="VMZ53_34575"
     CDS             complement(4921..5187)
                     /locus_tag="VMZ53_34575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33694.1"
                     /translation="MATLIDNPKRARQLARAIASDLTLYHEPKILEGISNDNLFEVMA
                     DEINEGRELFKSRVTEDIFGQHIYERAIVDVLIKSKGHVKSKIW"
     gene            complement(5220..6779)
                     /locus_tag="VMZ53_34580"
     CDS             complement(5220..6779)
                     /locus_tag="VMZ53_34580"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUS33695.1"
                     /translation="MTARVIAIAGGKGGVGKSLVAANVGIFLATLGKRVILIDGAFGA
                     PNLHIFAGVPRPSRSLAEALAAGGPPLAELAVATHVPGVKLIGGVYDPPWIANPGPAQ
                     VRVIAEQLHQLPADWVVVDLGPGLASPTLELFLEADVQVLVSVPDPTSIELMHRFVRA
                     AFLKRLERKGLSHLVQIVDKEPTELEGGTPSALDIYLAAVASQTELHELREAILGFSP
                     HLVINSARSKSDMELGRAVASAARRRLGTPIRYLGHLEYDEAVWASTRRRRPLLIEHP
                     ETRIAKCFERVARGIMAVRPSATDGDVLPSDSHYELLEVPPTASFEDIRRANRRIRDI
                     YGAESIAVSGLYDPASLEAVHRRLDLAYTTLMDAAKRKEYDHELFPDGVPMPVTPPQI
                     LADASAPATRPASKADDPAVASVRPPMPEITARTEFSGPMLRQIREAVGVELREIAEK
                     SKIGMAYLQALEGEVFAKLPAPVYVRGFLVEYARALGLDVERVKQTYLDRYRAARGPV
                     PEPPEDEAAKP"
     gene            complement(7611..8972)
                     /gene="selA"
                     /locus_tag="VMZ53_34585"
     CDS             complement(7611..8972)
                     /gene="selA"
                     /locus_tag="VMZ53_34585"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HUS33696.1"
                     /translation="MSDLRLAKLPAVHALADEAEALASIPRWAAVEAARRSINDRRAA
                     ILAGKADSPVIAPAEVAELAASLAAPPLRRVINATGVVLHTNLGRAPLAASARAAIDE
                     AARGYTNLEYDLVKGERGSRHDHLRTLLCELTGAEDAIVCNNNAAATVLGLAALATNK
                     EIIVSRGELIEIGGSFRLPEILALSRGTLVEVGTTNKTHARDYENAIGPNTGLLLKVH
                     RSNFAIVGFTAEVTPEQVVAIGRERSVATMIDLGSGMVIDRETQKRWGLPDEPTVRES
                     VQSGADLVTFSGDKLLGGPQAGIAVGKKAAVEAARKHPLMRALRPDKLTLAGLAATLS
                     LYRDGLAEDVPTVHMIARTSEALHADARRLLDMIGQTSLQLAVEGTTSTVGGGAMPTA
                     ELPSWAVTIAGTSPDALDRALRRHAPPIIGTIRDAKLWLDVRTIGEEELSLVAAALRS
                     LRP"
     gene            8999..10333
                     /locus_tag="VMZ53_34590"
     CDS             8999..10333
                     /locus_tag="VMZ53_34590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HUS33697.1"
                     /translation="MAWYCPRCGQTADAGGSCTRDGAALAEIGSHDLIGRAIGEYTVL
                     ARLGSGAFGQVYRAVHPRSGNVVAIKLLDQPIEAAESQRVITEARAAASIRHPNVVQV
                     YDLGITPKAEGDDFTSAPGRDRRPFIVMEYLEGQTLADAWVGRIPADEVVRVATDVLR
                     GLAAAHRLGIVHRDLKPANVFVSGPRVVIVDFGLAKLLGDPRGPALTIKGTSLGTPRY
                     MSPEQIRGQDIDGRTDLYALGVMMYEAIAGEPPFSAASTFQLLEQHIEKPPPELPKEL
                     PRHLTDAIDRALAKTAQERFPDADTMRRALRGDVESVHAARPRTSRSLAPILFALAGL
                     LAVTAIVIAVVRHGRGGDAKPRPAPAQPVAGRPVLTPEDQRVVETLRANVRSMPFADL
                     QRVLCNTEDGIATMTRYGADPYKVQVLRSFLQAYRTDPRTKAITCSDSSIRR"
     gene            10343..>11300
                     /locus_tag="VMZ53_34595"
     CDS             10343..>11300
                     /locus_tag="VMZ53_34595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33698.1"
                     /translation="MDVQAVSALLAAVLILAIGASVVLRSRSDRMYTAFAAFTFTVSA
                     WHLCSFIDAASSSPVMRWLSLWAAATIPPTAIRFYRVFLSQPSIGGPKRGPRVTLAWT
                     LIAYAALVYSAIVQPIHESKWFQIPFGLYVFGGLYRCVWDMYVQYRATTKRVERTRIG
                     YLALGGFVATTLTLTDVLPRFGIAWPAIGNVLGILYLYFLSQTLFRNRLLDLNELVGK
                     MAVLGTLVALLTAVYGFLFYWVGKSQRTDNLYLLNALVAAFVILILFEPVRSWLENSI
                     NRWLLRQRTELRGTIEAVRRDLPNIVDVGDMVQRIMTALEESR"
BASE COUNT         1826 a         3871 c         3776 g         1827 t
ORIGIN      
        1 tcggtcggcg tcatgtcggc gcaagtcgcg cacgagatca acaacccgct cacgaccgtg
       61 ctcgggtacg cgaagctctt gctcgaggac aagcccgagg gccatcccga tcgcaacgcg
      121 ctcgagctga tcgccgaaga agcggagcgg atgaagaaga tcatcggcgg cctgctggag
      181 tacgcgcgcg cgccacgccc ctcggagccg ctcacgcacg ttgcggccgg cgcatcggcg
      241 ccaggtgcga gcgagccgtc ggccgtcgtt cgccacgtcg cggcgctcgt cgcgccgcag
      301 ctcaaacgtg cgcgcgcatc gctcgtcacc gaggtaccga cgggcaagcc gatttcgatc
      361 gaggcgcacg cgctccagca ggtgctcgtc aacctcgtgc agaacgctgc gcaagcgctg
      421 tccgatggcg gaacgatcac gatctcgtcg aaagaagtcg caggcggaat cgcaacgcag
      481 atcactgtgg ccgacgacgg gcccggcatc ccggcggccg atcgcacgcg cgtgttcgat
      541 ccgttcttca cgacgaaggc gcccggcacg ggcacgggcc ttggcctcgc ggtgtgtcgt
      601 cacctcgtcg cgacggcggg cggctcgatc gaagcgcacg aaggccccgg cggtaagggc
      661 gccgagttcc gggtcgtgat tccgaacgtc tcttcataga cagtggcaat tcgctacggt
      721 ttcatcgaaa cctgcggaaa tgtgcggtgt cgatgaagca tgccggtcgt catcgaagtc
      781 gtcgcaaggc ttggcgacac gacgctcgat cacgatcaca tcccgccggg gagcacgtac
      841 cacctcggga ccacggcgct ggtcgcaaag cccggcacgc atcgcgtcgg cctcgtcgaa
      901 attacgatgg agcggacgtt tcgcgtcgaa acgccggtct cgcgcccgcg cctgacctgg
      961 cgcccgctca tctacgtgct cggctcgttc gcgctgcaca tgacgatctg gacgatcgcg
     1021 atgctggcga caccgatcga gcagatggag gtcgcgccca tcgatctgcg gccgcggttc
     1081 gtcgtccaca tcgaagaccg cacgccaccg ccgccgaaac cgcagcacga cattcaggca
     1141 cggcagccga ccgaatccgg cgcagcggct gtcgcgaagc agaagatgcc cgagcacggc
     1201 gcgacgccga cggccgcgga catgggtgat gcggctgcga aagcgatcgc ccgcgtcgct
     1261 cgctcggttc ccgatgtgaa caaggcgatg gaaggcaccg gcccgctcta cagcgaagac
     1321 gatgcgaccg cgaaaggctt cggcggccac ctgtgggaca tcaacagcga tcctgattac
     1381 gccacgatca agaccggtcc tggctacaac ctctatgaag ctgcgtactc gggcgagtgg
     1441 gacctcggca tgacgaagca agagaaggcg ctgagcaaga tcaaggcgca gacggcgctc
     1501 gcacataggg cggcaatccg cggcgactgc gtgccatcgc atcgcatcgc gaagtggctg
     1561 cacaagctcg acgaagccgc gttcaaagag atctatatgg gcgacccgga gatcgtgtgg
     1621 tgcctcggcc agccggtccc gcctgtattc gtcaaagcag atgctcagga agggctgaag
     1681 tcggcgatca aaccgcggta gtttcagagc gggatggccg agcagcccag gatgaagagc
     1741 ggaacgctcg ccggcgagat cgtcgccggt cacgagctca tgcatttgct cgcggtcggc
     1801 ggcatgggcc aggtctacct cgcgcgccat cttcgcctcg gcatcattcg cgccgtcaaa
     1861 gtcattcacg cggagctgct cgagcaagat cgatcgcgcg agcgcttcga acgcgaggct
     1921 cacgtgctcg cgcgcttgca gcacaactcg atcgtgcaca tcgtcgagta cggctcgctc
     1981 gacaacggca tgccgttcct cgcgatggag tacatcgatg gcccgacgct cgaacgactc
     2041 gtcgaggacg cgcccctccc gctgccgagc gcgctcgtcg tgctctctca gctcgcgaac
     2101 gcgatcaact acgcgcactc gtgcggcgtg attcatcgcg acctcaagcc gtcgaacgtc
     2161 ctcgtgcgtg gaggcgatgt acggcagctc aagatcatcg acttcggtct cgcgcacgtg
     2221 atcaacagcg atgcgccccg cctcaccgac gagaagcaga tgatcggctc ggtgacgtac
     2281 atggctcccg agcaagtcga acgcatccgc gaggtgacga cggctgtcga catctacggt
     2341 ctcggcggca tcgcgtatcg gttgatctct ggctcaccgc cgttcaagta ctccgcgccg
     2401 attcgcctca tgaccgcgcg gctgagcgag gagccagagc cgctcgctgc gcgatgtccg
     2461 gatattccgg ctgcgctcga ttcgctgctc acgcgctgtc ttgcccggga gcccaggcag
     2521 cggccatccg gcgaggagct catcacggag ctcgagcgac tgtcgcgagc gcgctaggcc
     2581 gcggcgccgt ctcgtgatac cccaggcgcg tgagcaggat cctcgttgtc gacgacgaac
     2641 gcggcattcg cgcgctgtgc tccgacgtcc tcggccgcgc gggccacgag gtcgagactg
     2701 tcgactccgc cgcgggcgcg ctcgccgcgg tcgctcgcaa aaagctcgac ctgatcctgt
     2761 gcgacatcaa cctcccggat caagacggcg tctcgatcct gcccaagctg ctcgcgggtg
     2821 aaacgccgcc ggcagtcctg ctgatcaccg catacccatc gatcgacacg gccgtacgcg
     2881 gcatgaagct cggcgcgcgc gactacatcg gcaagccgtt ctcgcccgac gagttgcgcc
     2941 tcgtcgtcca gcgcgcactc gccgaagacg agctgcgtcg caagcacggc gagctcacga
     3001 agcgcctcgc gtacggctcg atgatcggcg agtcgctagc gatgcagcag atgcgctcga
     3061 cgatcgacaa ggtcgcgcac tccgacgcga cggtgctcgt cacgggggag agcggaaccg
     3121 gcaaggagct cgtcgcacgc gcgctgcact tcgcaggtcg tcgcgccggc ggtgccttca
     3181 tcccggtcaa ctgcggcgct ctcgtcggca cgctgctcga tagcgagctg ttcggtcacg
     3241 ttcgcggcgc gttcacgggc gccgagacgt cgaagcgcgg cctgttcgtc gcagccgacg
     3301 gcggcacgct gttcctcgac gagatcggcg agcttccgct cgagctgcag cccaagctcc
     3361 tgcgcgcact ccaagacggc gaagtgaaac ccgtcggcgg cacgacctcg atccgtgtcg
     3421 acgcgcgcgt gatcgcagcg acgaatcgca cgctgcagga tcaaatcacc gcgggcgcat
     3481 tccgcgaaga cctctactac cgcctcgccg tgatcacgat cgaggtccct gccctgcgca
     3541 accgccccgg cgacatcccg ctactcgcgc gccacttcgc cgagcaagcg gccctccgcg
     3601 ccgaacgcgg ccgcgtgacg ctcaccgacg ctgcacttgc acatctcgcg agccaaccgt
     3661 ggcccggcaa cgtgcgcgag ctcgagaacg cgatcgagcg cgccgtgatc ctcgccgcgg
     3721 gcgacatcct cgatgtcagc gacgtggccg gtccgcgctc cgccgtcacg gcgccgacgt
     3781 tcgcgacgtt ctccggcgat cacgtgccga cgctcgacga gctcgagcgc gctcacatcc
     3841 tcaaggtgct cgagctgtgc gaaggccaga agacgaaggc tgcgcagatg ctcggcatca
     3901 atcgcacgac gctgtggaag aagctgcgcc agtacgggct cgacgccgat taacgcagcg
     3961 ccgcgagcgc cgattgcatg tcctcgggca gcgcggactc gaattcgagc tcgtcgcccg
     4021 tcgccggatg ggcgaatgca agcgtcgctg cgtgcagcgc ttggcggccg agcttcgttg
     4081 cgacgtccgc cgttcgctcg tcacgcggct tgtggccata gacctgatcg ccgatgagcg
     4141 cccagccgtg atcgctcgcg tgcacgcgga tctgatgcgt gcgtccggtt tccagacgaa
     4201 agcgcacgag tgcggcctcg tggagcggtt cctcgacggt ccagtgcgtg accgcggact
     4261 ttccgccgct gaccttcgac gagaacttct tgcggtgcac cggatggcgg ccgtactgcg
     4321 tgcgcagcgt gccggtcttc gtgggcggcg ccggcgaggt aatcgcgagg tacgtgcgct
     4381 cgaggccacc tggttcgccg cgcgatttgg ctgcgaacgc cgcggtgagt gccgcgtgcg
     4441 cgcgatcggt cttcgtcgcg accatcacgc cgctcgtgtc tttgtcgagg cgatggacaa
     4501 tgccggggcg aagcacgccg ccaatgccag cgaggtcttt gcagtggtgc agcagcgcgt
     4561 tgacgagcgt gccgcgcgca tggcctgcgg cgggatgcac gacgagcccc gccggcttgt
     4621 cgatcacgat gagatcggga tcctcgtaga gcacgacgag cggaatcggt tcgggcagca
     4681 cctcgagcgg ctcgggggcc ggcaccgtca cctcgatcgt gtcgccctgc ttgacgcgca
     4741 gcgacttgga ggcgaccgcg ccaccgaccg agacgtgacc gtcttcgatc aggcgctgga
     4801 cttgcgcgcg cgataacgct gcgagcgcac tggcgatgac cgcatcgagg cgtccccctg
     4861 cctgctctgc ggtgatgacg agctcgaaca gccgctcgtc gtcgtctccg tcctgcgtgt
     4921 ctaccagatc ttcgatttga cgtggccctt cgacttgatc agcacgtcga cgatcgctcg
     4981 ctcgtagatg tgctggccga agatgtcctc ggtcacgcgg ctcttgaaca gctcgcggcc
     5041 ttcgttgatc tcgtcggcca tcacctcgaa caggttgtcg ttcgagatgc cttcgaggat
     5101 cttcggctcg tgatagagcg tcaggtccga cgcaatggca cgcgcaagct gacgcgcacg
     5161 cttgggattg tcgatcaggg tcgccataaa gcgatcgtag gggcctggct aagcccgggt
     5221 caaggcttgg cggcttcgtc ctcgggcggc tcgggcacgg ggccgcgcgc cgcgcgatag
     5281 cgatcgagat acgtctgctt aacgcgttcg acatcgaggc ccagcgcccg tgcgtactcg
     5341 acgaggaagc cgcgcacgta gaccggcgcc ggcagcttcg cgaagacctc gccctcgagt
     5401 gcctgcaggt acgccatgcc gatcttcgac ttctcggcga tctcgcgcag ctcgacgccc
     5461 accgcctcgc ggatctgccg caacatcggt cccgagaact cggtgcgcgc ggtgatctcc
     5521 ggcatcggcg gccgcacgct cgccactgcc ggatcatccg ccttcgaggc aggccgcgta
     5581 gccggcgccg acgcatcggc gaggatctgc ggcggcgtca cgggcatcgg cacgccgtcg
     5641 gggaacagct cgtggtcgta ttccttgcgc ttcgccgcat ccatcagcgt cgtgtacgcg
     5701 agatcgagcc gccgatgcac cgcctccaag ctcgccggat cgtacaagcc cgacaccgcg
     5761 atcgactcgg cgccgtagat gtcgcgaatg cggcggttcg cacgccggat gtcttcgaag
     5821 ctcgcggtcg gcggcacttc gagcagctcg tagtgcgagt cgctcggcag cacatcgccg
     5881 tccgtcgccg acgggcgcac cgccatgatc ccgcgcgcga cgcgctcgaa gcacttcgca
     5941 atcctcgtct cgggatgctc gatgagcagc ggccgccgcc gccgcgtcga cgcccacacc
     6001 gcctcgtcgt actcgagatg cccgagatac cgaatcggcg tgccgaggcg ccggcgcgcc
     6061 gccgaggcca ccgcgcggcc gagctccata tcactcttgc tgcgcgccga attgatcacg
     6121 agatgcggcg agaaccccaa gatcgcttcg cgcaattcat gcagctcggt ctggctcgcc
     6181 acggccgcca aataaatgtc gagcgcgctc ggcgtaccac cctcgagctc cgtcggctcc
     6241 ttgtcgacga tctgcacgag atgcgacaag cccttgcgct cgagccgctt cagaaacgcc
     6301 gctcgcacga accgatgcat gagctcgatc gacgtcggat cgggcacgct gacgagcacc
     6361 tggacgtcag cctccagaaa cagctcgagc gtcggcgacg ccaaccccgg ccccaaatcc
     6421 acaacgaccc aatcggccgg cagctgatgc agctgctccg cgatcacgcg cacctgcgcc
     6481 gggccgggat tcgcgatcca cggcgggtca tacaccccac caatcagctt cacgccaggc
     6541 acgtgcgtag caacagccag ctccgccaac ggcggcccac ccgcggccaa cgcttcggcc
     6601 aggctgcgcg aaggccgcgg cacgcccgca aagatgtgca gattcggcgc gccgaacgca
     6661 ccgtcgatca gaatgacgcg cttgccgagc gtcgcgagaa agatgccgac gttcgcagcg
     6721 acgaggctct tgcctacgcc gcccttaccg cctgcgatcg cgatcacccg cgctgtcatg
     6781 tccgggcggc acataaccgc gactcccgtc gacggtcaag cccgccgttc cgtcggtggc
     6841 tcgcatcgcg ctcgcgccgc tcggcatcgc gctcgcgccg ctcggcatcg cgctcgcgcg
     6901 cgaggacggg cggcttcgcg gcagggccgt cgtcgccgcg tcagcgcggt ggggcggaca
     6961 gcgcgatccg ggcgctagtc gcggacgagg ctgcggggca cgcgcacttg cgaggggata
     7021 cgcgtcgccg agaccctgcc gcttcgccac ccgaagacgc gcgcgaacgc gatgcccgcg
     7081 atgcccgaga gcatcgccac cgcgatcaca tcgcccgccg acgggctccg ccggggcgct
     7141 gcgcggaccg ggaaggaacg cagaggcgca aagacagcaa aggccgcaat cggaggatca
     7201 cagggggcgt tccgcagagg cgcacgcagc gagccgtgcg cgcgccaagc ggcggcatac
     7261 gcgaactcgg cgatcgtgag cggcacacgc cctgcctcgc gccgaaggcg cgccccttct
     7321 ccttgcgccc tttgccctca ctgcgccttt gtgttccatc cggtccgcgg ctccgccgca
     7381 acacggcgcc gcaccacaac acagcgctgc gccgcacgcg gggcgcggca aacgcgatca
     7441 tgtggcggac gcgaacgcgc cgagcgccag cacacgccct gcctcgcgcc gaaggcgcgc
     7501 cccttctcct tgcggccttt gccctcactg cgtctttgtg ttccatccgg gtccgcggtc
     7561 gggcgcgcag cgcggcggcg ccgggcgcgc gacgtggcac gaggcggcga ctacggacgg
     7621 agcgagcgga gtgccgcggc gacgagggag agctcctcct cgccgatcgt gcggacatcg
     7681 agccaaagct ttgcgtcgcg gattgtgccg atgatgggcg gcgcgtggcg gcgaagggcg
     7741 cggtcgagcg cgtcgggcga ggtgcctgcg atcgtgacgg cccaggacgg cagctcggca
     7801 gtcggcatcg cgccaccacc gacggtggag gtcgtgccct cgacggcgag ctgcagcgac
     7861 gtttggccga tcatgtcgag gaggcggcgg gcgtcggcgt gcagcgcttc gctcgtgcgc
     7921 gcgatcatgt ggaccgtcgg gacgtcttcg gcgaggccgt cgcggtagag cgagagcgtg
     7981 gcggccaggc cggcgagcgt cagcttgtcg gggcgcagcg cgcgcatgag cgggtgcttg
     8041 cgtgcggctt cgaccgcggc ctttttgccg accgcgatgc cggcttgcgg accaccgagc
     8101 agcttgtcgc cgctgaatgt cacgaggtcg gcgccacttt ggaccgactc gcgcacggtg
     8161 ggctcgtcgg ggaggcccca gcgcttttgc gtttcgcggt cgatgaccat gccgctaccg
     8221 agatcgatca tcgtcgcgac gctgcgttcg cggccgatcg cgacgacttg ttcgggcgtg
     8281 acttcggcgg tgaagccgac gatcgcgaag ttcgagcgat ggactttgag gaggagtccg
     8341 gtgttcggac cgatcgcgtt ttcgtagtcg cgtgcgtgcg tcttgttcgt cgtgccgact
     8401 tcgacgagcg tgccgcgcga cagcgcgagg atttcgggga ggcggaacga gccgccgatt
     8461 tcgatcagct cgccgcgcga gacgatgatc tctttgttgg tcgcgagtgc ggcgaggccg
     8521 aggaccgttg cggctgcgtt gttgttgcag acgatcgcgt cttccgcgcc cgtgagctcg
     8581 cagaggagcg tgcgcaggtg atcgtgacgc gagccccgct cgcctttgac gaggtcgtac
     8641 tcgaggttcg tgtagccgcg ggcggcttcg tcgatggcgg cgcgcgcgga agcagcgagc
     8701 ggagcgcggc cgaggttcgt gtggagcacg acgcccgtcg cgttgatgac gcggcgaagc
     8761 ggcggcgcgg cgagagaggc cgcgagctcg gcaacttcgg ccggcgcgat gacggggcta
     8821 tcggctttgc ccgcgaggat ggcggcgcgg cggtcgttga tcgaacggcg tgcggcctcg
     8881 acggcggccc agcgcgggat gctggcgagg gcttccgcct cgtcggcgag agcatgaacg
     8941 gctggaagct tcgcgaggcg caggtccgac aagcagccag accatataag atcgcgcggt
     9001 ggcgtggtac tgcccgcgtt gcggtcaaac ggcggatgcc ggcggttcgt gcacgcgcga
     9061 cggcgcggcg ctcgccgaaa tcggcagcca cgatctcatc ggacgcgcga tcggcgagta
     9121 cacggtgctc gcacggctcg gcagcggcgc gttcggccag gtgtatcgcg ccgttcatcc
     9181 tcgcagcggc aacgtcgtcg cgatcaagct cctcgatcaa ccgatcgagg cggcggagag
     9241 ccagcgcgtg atcaccgagg cgcgcgcggc tgcgtcgatc cggcacccga atgtcgtgca
     9301 ggtctacgac ctcggcatca cgccgaaagc ggagggggac gacttcacgt cggcccccgg
     9361 gagagaccgc aggccgttca tcgtcatgga gtatctcgag ggacagacgc tcgccgatgc
     9421 gtgggttggc cgcattcccg ctgacgaagt cgtgcgcgtg gcgaccgatg tgctccgcgg
     9481 gctagcggcg gcgcatcgcc tcgggatcgt gcatcgcgat ctcaagccgg cgaacgtgtt
     9541 cgtcagcggg ccgcgcgttg tcatcgtcga cttcggcctc gcgaagctcc tcggcgatcc
     9601 gcgtgggccc gcactgacta tcaagggcac gagcctcggc acgccgcgct acatgtcgcc
     9661 ggagcagatc cgcggtcagg acatcgacgg ccgcacggat ctctacgcgc tcggcgtcat
     9721 gatgtacgag gcgatcgcag gggagccgcc gttctccgct gcctcgacgt tccagctcct
     9781 cgagcagcac atcgaaaagc cgccgcccga gctgccgaag gaattgccgc gtcatctcac
     9841 cgatgcgatc gatcgcgctc tcgcaaagac cgcgcaggaa cggttccccg acgccgacac
     9901 gatgcgacgc gcgctgcgcg gcgacgtcga atcggtccac gcagcgcgtc cgcggacctc
     9961 gcgctcgctc gctccgatct tgttcgcgct cgcgggcctt ctggcggtca cggcgatcgt
    10021 catcgcggtc gtacgccatg gccgcggtgg tgatgccaag ccgcgccctg ccccagcgca
    10081 accggtcgcc ggccgcccgg tgctcacacc cgaggatcag cgcgtcgtcg agaccttgag
    10141 agccaacgtt cgctcgatgc cgtttgccga tctgcaaagg gtcttgtgca acaccgagga
    10201 cggcatcgcc acgatgacgc ggtacggcgc cgacccgtac aaggtgcagg tcctgcgctc
    10261 gttcctccag gcataccgga ccgacccgcg gacgaaggcg atcacctgtt ccgactcgtc
    10321 gattcgacgc tagactgcgc tcgtggacgt acaggcggta tccgcgctgc tcgccgccgt
    10381 tctgatcctc gcgatcggcg cgtcggtggt gctgcgctcg cgatccgacc gcatgtacac
    10441 cgcattcgcg gcgttcacat tcacggtctc ggcctggcac ctgtgctcgt tcatcgacgc
    10501 ggcgtcgtcg agcccggtga tgcggtggct gtcgctgtgg gctgcagcga ccattccgcc
    10561 gaccgcgatc cgcttctatc gcgtgttctt gtcgcagccg tcgatcggcg gtccgaaacg
    10621 cggacctcgc gtgacgctcg cgtggacgct gatcgcgtac gccgcgctcg tgtactcggc
    10681 aatcgtccaa ccgatccacg agtcgaagtg gttccagatt ccgttcgggc tctacgtgtt
    10741 cggcggtctc taccgctgcg tttgggacat gtacgtgcag taccgcgcga cgacgaagcg
    10801 cgtcgaacgc acgcgcatcg gctatctcgc tctcggcggc tttgtcgcga cgacactaac
    10861 gctcaccgac gtgctgcctc ggttcggcat cgcgtggccc gcgatcggca acgtcctcgg
    10921 cattctctac ctctacttct tgtcgcagac gttgttccgc aatcgcctgc tcgacctcaa
    10981 cgagctcgtc ggcaagatgg ctgtgctcgg cacgctcgtc gcgctgctga cggccgtcta
    11041 cggcttcctg ttctactggg tcggcaagag ccagcgcacc gataacctgt atctgctcaa
    11101 cgcgctcgtc gccgcgttcg tgatcctcat cttgttcgag ccggtgcgca gctggctcga
    11161 gaacagcatc aaccgctggc tgctgcgaca acgaaccgag ctgcgcggaa cgatcgaggc
    11221 ggtgcggcgc gatctgccga acatcgtcga cgtcggcgac atggtgcagc ggatcatgac
    11281 ggcgctcgag gagtcgcggc
//
LOCUS       DATLBZ010000714         3692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6698608, whole genome shotgun sequence.
ACCESSION   DATLBZ010000714 DATLBZ010000000
VERSION     DATLBZ010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6698608"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VMZ53_34600"
     CDS             <1..786
                     /locus_tag="VMZ53_34600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUS33699.1"
                     /translation="DTILNRLAVYIEKAVKLRGQLKSAMYYPIGILVVAIGVIAVMLL
                     KVIPVFEAMYKDMGNAELPGATKFVIGISHSFLNQWYLFAGVIFGTIFGWIGLRRTEG
                     GTEATDRFLLRVPVIGPVLRKIVVARFTRTLGTLLASGVPILDALDICARTAGNRVVQ
                     KGILRAKDKISEGHDMAGPLAESRVFPTMVVQMIGVGEQTGAMDQMLSKIADFYEEEV
                     DTAVSGLTSLIEPIMMAFLGVVVGGLIIAMYLPIFKLAGNIQG"
     gene            791..2500
                     /locus_tag="VMZ53_34605"
     CDS             791..2500
                     /locus_tag="VMZ53_34605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUS33700.1"
                     /translation="MAEVASAAPPPALTPTLGASPGDSLVRAPATVAAPTATADVRRR
                     VARLLLLRTIVVSVVLGLSLWLLLHGEAPASAAVWLQSSIIAATYLSSIVFGVLLRQG
                     FAPGRVARPMLANDLVLTTALVYITGAAQSPYTFLYALTIVAAGALSYRRGAVVTTVA
                     SLATLIAISLLAWTRAIVLPLSAQVHPWDQTGLELVRTLGVNSAALIGVGALSFIFGD
                     QLQKSADTLATTRRAAAELLTLHQDIVRSLSSGLITTSPDGVVLTSNQAAADILRTTS
                     SRLVGRAIETVLPGIAGLIELKRGDLTVPTQDDDIVIGVTVSPLRDAHDQIIGRVINF
                     QDLTELKRLEQHARRTERMATVGQLAAGVAHEIRNPMASISGSIELLRSSPQASEDDK
                     TLMTIVQREVQRLNVLIGDLLDYANPRAKQTVDFDLASLVDETVQVARGDQNFSDIEL
                     TAHVDAPLPVHADPAKLRQVVWNLVRNAADAASSGGKHVRVTASKVADGARITVEDDG
                     TGIPESLVGRIFDPFVTTKQKGTGLGLATCHAIVTEHGGRIDVETEVGKGTKMIVTIP
                     ATS"
     gene            2524..2847
                     /locus_tag="VMZ53_34610"
     CDS             2524..2847
                     /locus_tag="VMZ53_34610"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HUS33701.1"
                     /translation="MQCPICTQALEGGELVMVCTTCHKTLGGAGLSVGSTGEFRVPTP
                     EMLAEAAQAEPGESPDAKETCSWCLKPESDVKKLIGRSGTALCNECVSLACDIMDAEL
                     GDNWR"
     gene            2922..3347
                     /locus_tag="VMZ53_34615"
     CDS             2922..3347
                     /locus_tag="VMZ53_34615"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HUS33702.1"
                     /translation="MDQLNSFLRGELSAVETYRMALDKLDANSASRFEVEACMQSHQQ
                     RVQLLREAVLACGGTPSDSSGPWGVFAKAVEGAAKAFGEKAAIAALEEGEDHGLKDYK
                     DIDDNELDMQTRTIVAAQLLPAQQATHNRISSLKKQLRS"
     gene            3391..>3692
                     /locus_tag="VMZ53_34620"
     CDS             3391..>3692
                     /locus_tag="VMZ53_34620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33703.1"
                     /translation="MGASGWSYYVPYDADFAAALGKLHDDVFERGEYYRRSKLRKPKS
                     IAHLRELNREEGTHSILDITRIGDRPSAPETHAGRLPPPDPEGQQRWFARWRERMGS"
BASE COUNT          651 a         1293 c         1131 g          617 t
ORIGIN      
        1 gacacgattc tgaaccgtct cgcggtctat atcgaaaagg ccgtgaagct gcgcggccag
       61 ctcaagagcg cgatgtacta cccgatcggc attctcgtcg tcgcaatcgg cgtcatcgcg
      121 gtcatgctgc tcaaggtcat ccccgtgttc gaagccatgt acaaagacat gggcaacgcc
      181 gagctgccgg gcgcgacgaa gttcgtcatc ggcatcagtc actcgttctt gaaccagtgg
      241 tacctgttcg ctggcgtcat cttcggcacg atcttcggct ggatcggtct acggcgcaca
      301 gaaggtggaa ccgaagcgac cgaccgcttc ctcctgcgcg tgcctgtcat cggcccggtg
      361 cttcgcaaga tcgtcgtcgc gcgcttcacc cgtacgctcg gcacgctgct cgcctccggt
      421 gtcccgatcc tcgacgcgct cgacatctgc gctcgcaccg ccggtaaccg cgtcgtgcag
      481 aagggcatcc ttcgcgccaa ggacaagatc tcggaaggtc acgacatggc aggcccactc
      541 gccgagtcgc gcgtgttccc gacgatggtc gtccagatga tcggcgtcgg cgagcaaacc
      601 ggcgccatgg atcagatgct ctcgaagatc gccgacttct acgaagaaga ggtcgacact
      661 gccgtttccg gtctcacctc gctcatcgag cccatcatga tggcgttcct cggcgtggtc
      721 gtcggtggtc tcatcatcgc gatgtacctg ccgatcttca aactcgccgg caacatccag
      781 ggctaggccc atggccgagg tcgccagcgc ggcgcctcca cctgcactga cgccaacgct
      841 cggtgcatcg ccgggtgact cgctcgttcg agcacccgcg accgtcgcgg ctcccacggc
      901 cacggcggat gttcggcggc gcgtcgcacg tctgctgctg ctgcgcacga tcgtcgtgtc
      961 ggtcgtgctc ggcctctcgc tgtggctgct tctgcacggt gaggcgccgg cgagtgcggc
     1021 ggtgtggctg cagtcctcga tcatcgcggc gacgtatctg tcgtcgatcg tgttcggcgt
     1081 gctgctgcgt cagggcttcg cgcccggccg cgttgcgcga ccgatgctcg cgaacgacct
     1141 cgtgctcacg acagcgctgg tctacatcac gggcgcagcg cagtcgccgt acacgttcct
     1201 ctacgcgctg accatcgtcg ctgcgggcgc gctctcgtat cgccgcggtg ccgtcgtcac
     1261 gacggtcgca tcgctcgcga cgctcatcgc gatctcactg ctcgcgtgga cgcgcgcgat
     1321 cgtactgccg ctctccgccc aggttcaccc atgggaccaa acgggcctcg agctcgtgcg
     1381 cacgctcggc gtcaactcgg ccgcgctcat cggcgttggc gcgctgtcgt tcatcttcgg
     1441 cgatcagctg cagaagtccg ccgacaccct cgcgacgacg cgacgcgccg ccgccgagct
     1501 gctcacgctg catcaagaca tcgtgcgctc tctctcgtcg ggtctcatca cgacgtcgcc
     1561 ggacggtgtg gtgctcactt cgaatcaggc cgccgccgat atcctccgca cgacgtcgtc
     1621 gcgcctcgtc ggtcgagcca tcgagacggt gctccccggc atcgcgggcc tcatcgagct
     1681 caagcgcggc gacctcaccg tgccgacgca ggacgacgac atcgtcatcg gcgtcaccgt
     1741 ctcgccgctg cgcgacgcgc acgaccagat catcggccgc gtcatcaact tccaggacct
     1801 caccgagctc aagcgcctcg agcagcacgc acgtcgcacc gaacgcatgg caacggtcgg
     1861 ccagctcgcc gcgggcgtcg cgcacgagat ccgcaacccg atggcgtcga tctcgggctc
     1921 gatcgagctg ctgcgctcgt cgccgcaagc atccgaagac gacaagacgc tgatgacgat
     1981 cgtgcagcgc gaagtgcagc gcctcaacgt gctgatcggc gacctgctcg actacgccaa
     2041 ccctcgcgcg aagcagacgg tcgacttcga tctcgcatcg ctcgtcgacg aaaccgtcca
     2101 agtcgcgcgc ggcgaccaga acttctccga catcgagctc accgcgcacg tcgatgcgcc
     2161 actgcccgtg cacgccgatc ccgcgaagct ccgtcaggtc gtctggaatc tcgttcgcaa
     2221 cgcggccgac gctgcgtcgt caggcggcaa gcacgtgcgc gtcaccgcct ccaaggtcgc
     2281 cgatggagca cgaatcaccg tcgaggacga cggcacgggc atccccgaat cgctcgtcgg
     2341 ccgcatcttc gacccattcg tgacgacgaa gcaaaagggc acgggcctcg gcctcgcgac
     2401 gtgccacgcg atcgtcaccg agcacggcgg ccgcatcgac gtcgagaccg aagtcggcaa
     2461 aggcacgaag atgatcgtga cgattcccgc gacctcctag cgcgccggtg ctactgtcgc
     2521 gccgtgcagt gtccgatttg cacgcaagca ctcgaaggtg gcgagctggt gatggtgtgc
     2581 accacgtgcc acaagacact cggcggcgcg ggcctcagcg ttggctcgac gggcgagttc
     2641 cgcgtgccga cgcccgagat gctcgccgaa gccgcccagg ccgagcccgg cgagtcgccc
     2701 gacgcgaaag aaacgtgctc gtggtgtctc aagcccgagt cggacgtgaa gaagctcatc
     2761 ggtcgcagcg gcacggcgct ctgcaatgaa tgcgtgtcgc tcgcgtgcga catcatggac
     2821 gccgagctcg gcgacaactg gcgataacgc gcttcagtgg ctccctgctt gcacctcttt
     2881 gcgtgcggaa aaggagcaac atcatgggta ccaaatccag cgtggatcag ctgaacagct
     2941 tcctgcgcgg tgagctgtcc gctgtcgaaa catatcgcat ggcgctcgac aagctcgacg
     3001 ccaacagcgc atcgcgcttc gaagtcgaag cgtgcatgca atcgcaccag caacgcgtcc
     3061 aacttctccg tgaagcggtg ctcgcgtgcg gcggcacgcc gtccgattcg tcgggaccgt
     3121 ggggcgtgtt cgcgaaagcc gtcgaaggcg ccgccaaagc attcggcgag aaggccgcga
     3181 tcgccgcgct cgaagagggt gaagaccacg gcctgaagga ctacaaagac atcgacgaca
     3241 acgagctcga catgcagacg cgcacgatcg tcgcggcgca gctactaccg gcgcagcaag
     3301 cgacgcacaa ccgcatcagc tcgctcaaaa agcagctgcg cagctaagat ttcgcgcgac
     3361 ttactcggcc atctacgctc ccatctgcat atgggcgcct ctggatggtc gtactacgtg
     3421 ccgtacgatg ccgacttcgc agcggcgctc ggcaagctgc acgacgacgt cttcgagcgc
     3481 ggcgagtact accgccgttc gaagctgcgc aagccgaaga gcatcgcgca cctccgcgag
     3541 ctcaaccgcg aagaaggcac gcactcgatc ctcgacatca cgcggatcgg cgaccgcccg
     3601 tcggcgcccg aaacccacgc aggtcgcctg ccaccgcccg atcccgaagg ccagcagcga
     3661 tggttcgccc gctggcgcga gcggatgggc tc
//
LOCUS       DATLBZ010000715         3581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6701995, whole genome shotgun sequence.
ACCESSION   DATLBZ010000715 DATLBZ010000000
VERSION     DATLBZ010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6701995"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..2256)
                     /locus_tag="VMZ53_34625"
     CDS             complement(79..2256)
                     /locus_tag="VMZ53_34625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HUS33704.1"
                     /translation="MSKAGGLESKRKRVGNAPSSAASTVLAKGTRNLPKLARGTRNQL
                     PPFRPKEKPVVAKPEKLELDESLLDLETPDPDTDDTSTDPATAARDAEVDARMLELGQ
                     RRFGISHFRPGQALAIRNVIAGIDTLAIMPTGAGKSLCYQLPALELDGVTLVVSPLIA
                     LMKDQYDKLDQLQIEVLRLDSTLSPRDEQAALARLSENRPCIAYVTPERLGEPRFRER
                     LASVRVALFVVDEAHCISQWGHDFRPAYLGLGEAVRALGRPPVLALTATAPPKVKDDI
                     LAQLQLNIPPAEASVIDIGLDRPNLRYHVVKASSERKKQALLLRLIDKQEGCGIIYAA
                     TVKTVDALADFLWSQGVECGRYHGRMRAKDRERVQSAFMERSEPRLMVATNAFGLGVD
                     KQDLRFVIHYNFPGSLESYYQEAGRAGRDGLPADCVLLYQPEDKRIQSFFLGGRYPTP
                     EQTRAVAEALVYVTQGKVAQVDADGDPVGLDEDDSMFVSVKDIAERADAPAKKARVVL
                     AFLKEVGFAAEAPGARFAPLATEPPTIDDLARAATRYEQKRAQDRARLASMLRYSQSH
                     LCRTRLLVTYFGYTDAPACGNCDNCIRSNVTPSRTLTGNGHAMPNGNGNGNGNGNGNG
                     HTGEAAQTLAARRGGAADPDTRRALLEEAMRRNRSAGRNRALKIERVKRPVFGPLEKG
                     NIVRHATWGEGEVVRVVGDSVYTFFPVHGEKLLKTSFLEKISA"
     gene            2534..3028
                     /locus_tag="VMZ53_34630"
     CDS             2534..3028
                     /locus_tag="VMZ53_34630"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HUS33705.1"
                     /translation="MPLLDGLRVVHSKIHGYGVIATRRFVAGELVCFGDGVLYPEDAE
                     FDDTYALILDGEDSGKGETLFWDLACQTRWFNHACEPNTEVCSRWDHEADTMVAWWVA
                     LRDIEIGEEITYDYAFTAEVAEPCACGTSSCRGVIVDDDPENLAKLPDSLKQLLRGPQ
                     RAAS"
     gene            3078..>3581
                     /locus_tag="VMZ53_34635"
     CDS             3078..>3581
                     /locus_tag="VMZ53_34635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HUS33706.1"
                     /translation="MKKLRVGVLGATGMVGQRFVALLAEHPWFEVACVAASPSSAGKP
                     YQDAVAGRWTLATPVPSATAAMTVVDASDISKVADQVDFVFCAVDMDKAVTAKLEEDY
                     ARAETPVVSNNSAHRWTPDVPMMIPEINSDHQAVIEAQRKRLGTSRGFIAVKPNCSIQ
                     SYVPGIHP"
BASE COUNT          576 a         1140 c         1239 g          626 t
ORIGIN      
        1 aagatcgcgg cgactgattg ccgtcgaatc gccgtcaagc tgcgaaccac gacatcgttg
       61 ccgcgggtcg ggcgcgacct acgcgctgat cttctcgagg aagctggtct tcagcagctt
      121 ctcgccgtgc accgggaaga acgtgtagac gctgtcgccg accacgcgca cgacttcgcc
      181 ttcgccccac gtcgcgtggc ggacgatgtt gcccttctcg agcggtccga acacgggacg
      241 cttgacgcgt tcgatcttga gtgcgcgatt gcggcccgca ctgcgattgc gccgcatggc
      301 ctcctcgagc aaggcgcggc gcgtatccgg atccgccgca ccaccacgac gggcagcgag
      361 cgtctgcgcc gcctcgccgg tgtggccgtt gccgttaccg ttaccgttac cgttaccgtt
      421 gccgttcggc atcgcatggc cgttgccggt cagcgtgcgc gatggcgtga cgttgctgcg
      481 gatgcagttg tcgcagtttc cgcaggcggg ggcgtcggtg taaccgaagt acgtcacgag
      541 caagcgcgtg cggcacagat gcgattgcga gtaccgcagc atcgacgcga ggcgcgcgcg
      601 gtcctgcgca cgtttctgct cgtagcgggt cgccgcgcgt gcgaggtcgt cgatggtcgg
      661 tggctcggtc gcgagcggcg cgaaccgcgc acccggtgcc tcggcggcga agccgacctc
      721 cttcaagaac gccagtacga cgcgcgcctt cttggccggt gcgtcggcgc gctcggcgat
      781 gtccttcacg gagacgaaca tgctgtcgtc ctcgtcgaga cccaccggat caccgtcggc
      841 atcgacctgc gcgaccttgc cctgggtcac gtacacgagc gcctcggcga ccgcgcgcgt
      901 ctgctcgggc gtcggatagc gaccgccgag gaagaagctc tggatccgct tgtcctcggg
      961 ctgatagagc agcacgcagt cggcgggcaa gccgtcacga cccgcgcgac cggcctcctg
     1021 gtagtagctc tcgagcgagc ccgggaagtt gtaatgaatg acgaagcgca ggtcctgctt
     1081 gtcgacgccg agcccgaacg cgttcgtggc gaccatgagc cgcggctcgc tgcgctccat
     1141 gaacgcgctc tgcacgcgct cgcgatcttt ggcgcgcatg cggccgtgat agcgaccgca
     1201 ctcgacgccc tgcgaccaca agaagtcggc gagcgcatcg acggtcttca ccgtcgcggc
     1261 gtagatgatg ccgcacccct cttgcttgtc gatgagacgc agcagcagcg cttgcttctt
     1321 gcgctcgctc gacgccttga cgacgtggta gcgcaggttc ggccgatcga ggccgatgtc
     1381 gatcaccgac gcttccgccg gagggatatt gagctggagc tgcgcgagga tgtcgtcctt
     1441 gaccttgggc ggcgcggtcg cggtcagcgc gaggaccggc gggcgaccga gcgcgcgcac
     1501 ggcctcgccg agcccgaggt acgcggggcg gaagtcgtgc ccccattgcg agatgcagtg
     1561 cgcctcgtcg acgacgaaca gcgcgacccg gacggaagcg aggcgttcgc gaaagcgtgg
     1621 ctcgccgagt cgctcgggcg tgacgtacgc gatgcacggg cggttctccg aaaggcgcgc
     1681 gagcgcggcc tgctcgtcgc gcggcgacag cgtggagtcg aggcgcagca cttcgatctg
     1741 cagttgatcg agcttgtcgt actgatcctt catgagcgcg atcagcggcg agacaacgag
     1801 cgtcacgcca tcgagctcga gcgcgggtag ctggtagcag agcgacttgc ccgcgccggt
     1861 cggcatgatc gcgagcgtgt cgatgccggc gatgacgttg cggatcgcga gggcctgacc
     1921 tggacggaaa tgcgagatgc cgaagcggcg ctggccgagc tcgagcatgc gagcgtcgac
     1981 ctcggcgtcg cgggcggccg tcgccgggtc ggtgctcgtg tcgtcggtgt ccggatcggg
     2041 cgtctcgaga tcgaggagcg actcgtcgag ctcgagcttc tcgggcttgg cgacgaccgg
     2101 cttctccttg ggacggaacg gagggagctg gttgcgggtg ccgcgggcca gcttggggag
     2161 attccgggtg cccttggcga ggacggtgga agcagcagag ctcggtgcgt ttccgactcg
     2221 cttgcgcttg ctctctaggc ccccagcttt ggacatcaaa cgcaaagttc gccggaaacc
     2281 actaacaaga caagggaaac atcgtgagga aactgccgtg cgaatgcgct cggtgactgt
     2341 tcgtgcgccc acaaatgcgc gatcaatccc gtcgtaaacg cgggcattgc agtggcactg
     2401 gatcgtgtct cagaagcgca ttgcggcttt tacgcgcgtt cacttcgagt cgaaacgggt
     2461 ctgcgcgcgc gaatcttcgt cggacgatcc cgctgacttg cacatggccg gcggatcgtg
     2521 atgatcgcgt gcgatgccgt tgctcgatgg tctgcgcgtc gttcattcca agatccatgg
     2581 ctacggcgtc atcgccacgc gccgcttcgt cgccggcgag ctcgtgtgtt tcggtgacgg
     2641 tgtcctctat ccggaggacg cggagttcga cgacacgtac gcgctgatcc tcgacggcga
     2701 ggactcggga aaaggcgaga cgctgttctg ggacctcgcg tgtcagacgc gctggttcaa
     2761 tcacgcgtgc gagccgaaca ccgaggtctg ctcgcgctgg gatcacgaag ccgacacgat
     2821 ggtcgcgtgg tgggtcgcac tgcgcgacat cgagatcggc gaggagatca cgtacgacta
     2881 cgcgttcacc gccgaggtcg ccgagccatg cgcgtgcggc accagctcgt gccgcggcgt
     2941 gatcgtcgac gacgatccgg agaatctcgc gaagctgcca gactcgctga agcagctgct
     3001 ccgcggtccg caacgcgccg cgagctgagc ttcgtctcgg cgcgctttgc cgacatagat
     3061 gtggtaactc gatcggtgtg aagaagctcc gcgtcggtgt cctcggtgcg accggcatgg
     3121 tcggccagcg tttcgtcgct ctgctcgcag agcatccgtg gttcgaggtc gcgtgcgtgg
     3181 ccgcgagccc gagctccgcc ggcaagccgt accaggatgc ggtcgccggg cgctggacgc
     3241 tcgccacgcc ggtgccatcg gcgacggcgg cgatgaccgt cgtggacgcg agcgacatct
     3301 ccaaggtcgc cgatcaggtc gacttcgtgt tctgcgcggt cgacatggac aaggctgtca
     3361 ccgcgaagct cgaagaggat tacgcacgcg ccgagacgcc ggtcgtgtcg aacaactcgg
     3421 cgcatcgctg gacgcccgac gtgccgatga tgattcccga gatcaactcc gatcatcagg
     3481 cggtgatcga agcgcagcgc aagcggctcg gcacctcgcg cggcttcatc gcggtgaagc
     3541 cgaactgctc gatccagagc tacgtgccgg gcatccatcc g
//
LOCUS       DATLBZ010000716         3896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U14311
            k141_6702140, whole genome shotgun sequence.
ACCESSION   DATLBZ010000716 DATLBZ010000000
VERSION     DATLBZ010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938790
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:50:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,930
            CDSs (total)                      :: 6,884
            Genes (coding)                    :: 6,863
            CDSs (with protein)               :: 6,863
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 19 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6702140"
                     /isolate="SMAG_U14311"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VMZ53_34640"
     CDS             complement(<1..776)
                     /locus_tag="VMZ53_34640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33707.1"
                     /translation="MRLSAASCLAIGLFGVAACSSFEDALPEDSEDGTVVEDDKADNF
                     YSLKAKEFIVSGTGRVVVAENATDAQVRKAIALEHTAITWFLNAYLVDKEREGMHSDP
                     NADYGGFSAMVKNGSYEDFQITKRNATTYDFAFQQTVAGKKTLMRSLPLNARSEFTIE
                     IGKPTVEEMQRDSEWYRKAPWDNWNPATVAAEKKETLTLAIKEDTKSSDGWWDYKRLF
                     EDGKLTIDAHFGYDYNPEAAHLTDSKAFYNWLVARGFKSPV"
     gene            884..1822
                     /locus_tag="VMZ53_34645"
     CDS             884..1822
                     /locus_tag="VMZ53_34645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33708.1"
                     /translation="MRGWLFLTLAACNFEHGALGTTGGSDDAMPGDDGRRAIDAMVSV
                     YAPPEGACLGKWKAGPTFTDPVMLANVNTTGDEADPFVSADEQTLYFVRTSDIYKATR
                     TSLTASFQNVAKAIDLSSSQGDSKVSLTADGLTAFVNTARTGGAGGATDIWRMTRSST
                     ANAFANPDQMYLMNVNGPSDAWDPHVSANGLRIYFAPPATPAQHIVVTERDSTSDPFG
                     TPKPITELDSGEKDNDPTLTADERLIVWASNRGGNRNLWYALRDDPTQPFGTPTVVPQ
                     INTNVDDGPHISNDGCRLYFTSDRDGDDDVWFTELQ"
     gene            complement(1824..>3896)
                     /locus_tag="VMZ53_34650"
     CDS             complement(1824..>3896)
                     /locus_tag="VMZ53_34650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUS33709.1"
                     /translation="LADAPEGTPPEVLAKLVYRYALATSVLGDRDGAHQLLHEAHHLS
                     RRDLHITLALGESCLARKLWREAAIHLGSLAEHPEASRNAAAVAAGLVKAGQAEVRAL
                     KPANAAKHYDAAVKLDPTCAPAWHQLAELATERGDTVRAAEHLEREAAATVEPNARVR
                     LYDALGDLALDVLGDAERAERCWSAVAQIATADMLTKLLALQRKRGATIERGETCERL
                     ALLTADKRAAKELTEEAAQAFAAGGDRDRARAVAEFLVAQHSLDADTIFAATSIAAGE
                     HEIVATWLRRALGAWDAARDKGEGDARRAELWRRLGDAEKGRRNERGALDAYQRAVNT
                     APDSDGALAARRGLIELAASFGRSANTSRMALVEAEQDPDDVIGTARELARSGDPDDA
                     RTMFELARALGVELLGEDESFLDAHTPRAMASDEAYAAALSDAERHTLIDDPADGVLA
                     DVFALLGEAGPLVCPDAKTALDNEALSDARRIPATSDAAAVAIYPQIAKALGGPATLM
                     YANPSRNAPDLRLLLASPPVLVLGPRLAGIRARSRSDAEYDVDSELRFRLGRAVELSR
                     PHRLFALGSDPEVYVKLVAALLHAFGPKSSDSDRHIAIEGERLRKSVPVALRSKLAEK
                     LATSPPLDATAYLAACERAADRAGLVACGHPGIAIHLAGGADRARHLVRLAASQAYLA
                     AQKKLRRR"
BASE COUNT          630 a         1249 c         1353 g          664 t
ORIGIN      
        1 accggtgact tgaagccgcg ggcgacgagc cagttgtaga acgccttgga gtcggtgagg
       61 tgcgcggcct ctgggttgta gtcgtagccg aagtgcgcgt cgatcgtgag cttgccgtcc
      121 tcgaacaggc gcttgtagtc ccaccaaccg tcgctcgact tcgtgtcctc tttgatcgcg
      181 agcgtgagcg tctctttctt ctcggcggca acggtcgctg ggttccagtt gtcccacggc
      241 gccttgcgat accactcgct gtcgcgctgc atctcttcga cggtcggctt gccgatctcg
      301 atcgtgaact cgctgcgtgc gttgagcggc aggctgcgca tcagcgtctt cttgccggcg
      361 accgtctgct ggaatgcgaa gtcgtaggtc gtcgcgttgc gcttcgtgat ctggaagtct
      421 tcgtacgagc cgttcttcac catcgcggag aagccgccgt agtcagcgtt cggatcggag
      481 tgcatgccct cgcgctcttt atcgacgagg tacgcgttga gaaaccacgt gatcgcggtg
      541 tgctcgagcg cgatcgcctt gcggacttgg gcgtccgtcg cgttctcggc gacgacgacg
      601 cggccggtac ccgagacgat gaactccttc gccttgagcg agtagaagtt gtctgccttg
      661 tcgtcctcga cgaccgtgcc gtcctcggag tcctccggga gcgcgtcctc gaacgacgag
      721 caggcggcta ctccgaagag cccgatggcg aggcaactag ccgcagacaa acgcatgcgg
      781 acggttgttt gcacagctgg taccagacgc ccacacagtg gatcctgggg gttgccgagt
      841 ggtggctgct gcaggcgcca gctggggatg agaccatggt ccggtgagag gttggctgtt
      901 cctgacgttg gcagcgtgca acttcgagca cggcgcgctc gggaccaccg gcggtagcga
      961 cgacgcgatg cccggcgacg acggccggcg cgcgatcgac gcgatggtga gcgtctacgc
     1021 acctcccgaa ggtgcgtgtc tcgggaagtg gaaagcgggg ccgacgttca ccgatccggt
     1081 catgctcgcg aacgtcaaca cgaccggcga cgaagccgat ccgttcgtgt cggcggatga
     1141 gcaaacgctc tacttcgttc gcacgtccga tatctataag gcgacccgca cgtcgctgac
     1201 cgcgagcttc cagaacgtcg cgaaggcgat tgacctatcg tcgagtcagg gcgattcgaa
     1261 agtgtcgctc acggcggacg ggctgaccgc gttcgtcaac acggcgcgca cgggcggagc
     1321 aggcggtgcg acggacatct ggcgcatgac acgaagctcg acggcgaacg cgttcgcgaa
     1381 tcccgaccag atgtatctga tgaacgtgaa cggaccgagc gatgcgtggg atccgcacgt
     1441 ttcggcgaat ggcctgcgca tctacttcgc gccgccggcc acgccagcgc agcacatcgt
     1501 cgtgacggag cgcgactcga cgagcgaccc gttcggcacg ccgaaaccga tcaccgagct
     1561 cgatagcggc gagaaggaca acgatccgac gctcaccgcc gacgagcgct tgatcgtgtg
     1621 ggcgtccaat cgcggcggca atcgcaatct ctggtacgcg ctgcgcgatg atccgacgca
     1681 gccattcggg acgccgaccg tcgtgccgca gatcaacacg aacgtcgacg acggcccaca
     1741 catctcgaac gatggttgcc gcctctactt cacgtcggat cgcgacggcg acgacgacgt
     1801 ctggttcacc gagctgcagt gagctagcgg cggcgcagct ttttttgagc ggcgagatac
     1861 gcctgcgatg cggcaaggcg cacgagatgg cgcgcacgat cggcgccacc cgcgaggtga
     1921 atcgcgatgc ccggatggcc acacgcgacg agacctgcgc gatcagcggc gcgctcgcat
     1981 gctgcgaggt acgcggttgc atcgagcggc gggctcgttg cgagcttctc ggcgagcttg
     2041 ctgcgcagtg cgacgggcac ggacttgcgg aggcgctctc cttcgatcgc gatgtgacga
     2101 tcggagtcac tcgacttggg accgaacgcg tggagtagcg cagcgacgag cttgacgtag
     2161 acttcgggat cgctgccgag cgcgaacagc cgatgcgggc gtgagagctc gacggcgcgg
     2221 ccgaggcgga agcgcagctc ggagtcgacg tcgtactcgg cgtcgctgcg cgagcgagcg
     2281 cgaatgccgg caaggcgggg cccgaggacg agcaccggtg gggacgcgag caagaggcga
     2341 aggtcgggcg cgttgcgcga cggattcgcg tacatcagcg tcgctggacc gccgagcgct
     2401 tttgcgatct gcggatagat cgcgacggct gcggcatcgc tcgtcgctgg aatgcgccgc
     2461 gcgtcggaga gcgcctcgtt gtccagcgcg gtcttcgcat ccgggcagac gagcggacct
     2521 gcttcgccga gcagcgcgaa cacatccgcg aggacgccgt ccgcgggatc gtcgatcagc
     2581 gtgtgtcgtt cggcatcaga gagcgctgct gcgtacgctt cgtcagaggc catcgcgcgc
     2641 ggcgtgtggg cgtcgaggaa gctctcgtcc tcaccgagga gctcgacgcc aagcgcacgg
     2701 gcaagctcga acatcgtgcg cgcgtcgtct ggatcgccgc tgcgtgcgag ctcgcgcgcg
     2761 gtgccgatga catcgtcggg atcctgctcg gcttcgacga gcgccatgcg cgatgtgttc
     2821 gcgctgcggc caaacgatgc agccagctcg atgaggccgc ggcgagcggc gagggcgcca
     2881 tcggaatccg gcgcggtatt gacggcgcgt tgataggcat cgagagcacc gcgctcgttg
     2941 cggcggcctt tctcggcatc gccgaggcga cgccacagct ccgcccgtcg agcatcgcct
     3001 tcgcccttgt cccgtgccgc atcccacgcg ccgagtgcgc gacgcagcca cgtagcgacg
     3061 atctcgtgct cgccggcggc gatgctcgtt gcagcgaaga tcgtgtcggc atcgagcgag
     3121 tgctgcgcga cgaggaattc ggcgacggca cgcgctcgat cgcggtcacc acccgccgca
     3181 aatgcctgcg cggcctcttc cgtgagctcc ttggcagcgc gcttgtcggc tgtgagtaac
     3241 gcgaggcgct cgcatgtctc gccacgctcg atcgtcgcgc cgcgcttgcg ctgcagggcg
     3301 agcagcttcg tgagcatgtc ggcggtagcg atctgcgcga cggcggacca gcagcgctcc
     3361 gcgcgttctg catcgccgag aacatcgagc gcgagatcgc cgagggcgtc atacaagcgc
     3421 acgcgcgcat tcggctcgac ggtggcggcg gcttcgcgct cgagatgctc ggcggcgcgc
     3481 acggtgtcac cacgctccgt cgcaagctcg gcgagctgat gccacgccgg tgcgcacgtg
     3541 ggatcgagct tcaccgcggc gtcgtagtgc ttcgcggcgt tcgccggctt gagcgcgcgc
     3601 acttctgctt gcccggcctt cacgagcccg gctgcgaccg cagccgcatt gcgcgacgcc
     3661 tctggatgct cggcgagcga gccgaggtgg atcgcggctt cgcgccacag cttgcgcgcc
     3721 aagcagctct cgccgagagc cagcgtgatg tgcagatcgc ggcgcgacag gtgatgcgct
     3781 tcgtgcagca gctgatgcgc accgtcgcga tcaccgagga cgctcgtcgc gagcgcatac
     3841 cgatatacga gctttgcgag cacctccggc ggcgttccct cgggcgcgtc ggcgag
//
